LOCUS       DASWNJ010000001         4438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_176602, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000001 DASWNJ010000000
VERSION     DASWNJ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176602"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1474)
                     /gene="tssI"
                     /locus_tag="VGB85_00005"
     CDS             complement(<1..1474)
                     /gene="tssI"
                     /locus_tag="VGB85_00005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HEY0132414.1"
                     /translation="MPTATDLWDTVTRSHKPLPSVRYSFRVEGWRGALWHVRRVELVE
                     ALSEPYRLTVELVVDEPRLDLDELLGDVCALELDRKTSTRTVHGVVLSATDLGRSAGR
                     HHVRLEVGPALALLAQRVDTRAWQDRNAPDILKDVLSGPLAELGRTINLDALRVGDYP
                     RREYCVQFRESDLAFAERLMQEEGITYYFDHSGEAEKLILIDDNHDFAALQGDNPIAI
                     RETEPDTAAAESVQRFSFGRRMRSSAVAQRNFDWQSPQSPRASARNGTDHRGRTREVY
                     EHDDIVYPGDGERLAKLKQERLDVGAHAASGESNVTGLYPGAWFSLQGHARAELDDKY
                     LVTKVIHKGEAPDVNIMVTQASAGPPYVNEFEVIPLKVPYRPMKSQQKPKVSGLQTAT
                     VTGPPGEEVHCDEHGRIKILPHWDRMSPQDETSSWWVRCAQALAGSGWGFFALPRIGM
                     EVVVDFLDGDPDRPLVVGCVYNGQNQPPYPLPAEKTKTTLK"
     gene            complement(1538..3268)
                     /gene="tssA"
                     /locus_tag="VGB85_00010"
     CDS             complement(1538..3268)
                     /gene="tssA"
                     /locus_tag="VGB85_00010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HEY0132415.1"
                     /translation="MAQSPDSPEVARARTSAQALLAPVPGDARAGKDAREDPRAELLR
                     DEMLKDEYARRNWPVIEEASRGLLTSVTKDLVIATFWAGAAFETDGLRGLADGLALLD
                     GLLRDYWEDMFPPVARRKARENALDNFYQHIGTRLGAHATAEPDEAGILKACAALLEQ
                     LHTTAQTRYSPPPPNRTVRDALTRLLATIPEAKPAEPEPPPVAAAPERPKAEPPKPPP
                     PKPAPPAPVPSDSVEPPKAGASVDDLLKSLGAATQHFSKIASGLRRQQPADPLAYRLL
                     RTAIWLRASEPVADAQGRAPVEPLPPATRSQCEAMARAGRWAELLDASESALAQAPQR
                     FALDLQRFSAAALGALDHKLARTAVLAEVTALLRRMPTLPTLQARDGTPLADSETLAF
                     LAAENLHGAAASPDPIAWAPMPAPPVAPPPPSPRPPNLSPTPGPQPPPNLSSAPGPSG
                     QASDEVASARRRLATGDRAGAIAQLQLVADTAASPRDRFEKRTALALLTLEAGLVPVA
                     RAQLAALEREAHERRLADWEPRLVSQILSALVTCLRASKVPADVAELQRVFDQLCVLD
                     PALAAGQSTR"
     gene            complement(3272..>4438)
                     /gene="tssC"
                     /locus_tag="VGB85_00015"
     CDS             complement(3272..>4438)
                     /gene="tssC"
                     /locus_tag="VGB85_00015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HEY0132416.1"
                     /translation="VDFTQNIRLAVLNCTKNDLLNDFEDAGKVEKSTLFRRVYTDGIG
                     VYGGKPYGLVCSNFDFGPSGNDIDLLRNCAAVSTMAHAPFIANASTEFFGKGETSFEV
                     LTKMRDMDSLFEMPQYARWRSFRESEDARNVGLCLPRFMLRAPYSANDRRVTSFNFTE
                     DTGGEHGGYLWGHSSIAFTSRVAASFAKYRWCPNIIGPNSGGAVEDLPIHEYEVLGKQ
                     HHKMPIEVQISDRREFELANNGFISLVHQKESDRGCFFSANSVQKVPRTNDVELQQSL
                     ELGAKLPYMFIVSRMAHYLKIRQRELLGSDMSRSQLEAELNKWIGNYVNNMETTDSKT
                     RASKPLRWAKITVEDVAGEPGRYTCRLQLRPHFKYNGAAFTLSLVGKVEKQAGK"
BASE COUNT          594 a         1311 c         1794 g          739 t
ORIGIN      
        1 acttgagggt ggtcttggtc ttctcggcgg gcagcggata cggcggctgg ttctggccgt
       61 tgtagacgca accgacgacg agcgggcggt cggggtcgcc gtcgaggaag tcgacgacga
      121 cctccatgcc gatccgcggg agcgcgaaga agccccagcc gctgccggcc agcgcctgcg
      181 cgcagcggac ccaccacgag gaggtctcgt cctgcggcga catgcgatcc cagtgcggca
      241 agatcttgat gcggccgtgc tcgtcgcagt ggacctcctc gccgggcggg ccagtgacgg
      301 tcgcggtctg caggcccgag accttgggct tttgctgcga cttcatcggc cggtacggga
      361 ccttgagcgg gatgacctcg aactcgttga cgtacggcgg gccggcgctg gcctgggtga
      421 ccatgatgtt gacgtccggc gcctcgccct tgtgaatgac cttggtgacg aggtacttgt
      481 cgtcgagctc ggcgcgcgcg tggccctgca aggagaacca tgcgccgggg tacaggccgg
      541 tcacgttgct ctcgccgctg gccgcgtggg cgccgacgtc gaggcgctcc tgcttgagct
      601 tggcgaggcg ctcgccgtcg ccggggtaga cgatgtcgtc gtgctcgtag acctcgcggg
      661 tgcggccgcg gtggtcggtg ccgttgcggg cgctggcgcg cggggactgc ggggactgcc
      721 agtcgaagtt gcgctgggcg acggcggagg agcgcatgcg ccggccgaag gagaagcgct
      781 gcacggactc ggcggcggcg gtgtcgggct cggtctcgcg gatcgcgatc gggttgtcgc
      841 cctgcagggc ggcgaagtca tggttgtcgt cgatcaggat cagcttctcg gcctcgccgc
      901 tgtgatcgaa gtagtaggtg atgccctcct cctgcatcag ccgctcggcg aaggcgaggt
      961 cggactcgcg gaactggacg cagtactcgc ggcgcggata atcgccgacg cgcaaagcat
     1021 cgaggttgat cgtgcggccg agctcggcga gcgggcccga gagcacatct ttcaaaatgt
     1081 cgggggcgtt gcggtcctgc caggcgcggg tgtcgacgcg ctgggcgagc aaggcgaggg
     1141 ccggcccgac ctcgaggcgg acgtgatggc ggccggcgga gcggccgagg tcggtggcgg
     1201 agagcacgac gccgtgcacg gtgcgggtgc tggtcttgcg gtcgagctcg agggcacaga
     1261 cgtcgccgag cagctcgtcg aggtcgaggc ggggctcgtc gacgacgagc tcgacggtga
     1321 ggcggtaggg ctcgctgagg gcctcgacca gctcgacgcg gcgcacgtgc cacagggcgc
     1381 cgcgccagcc ctcgacgcgg aagctgtagc gcaccgaggg gagcggcttg tgggatcgcg
     1441 tgacggtgtc ccagaggtcg gtggcggtcg gcatggcgat gtccttttgc ttttttaaaa
     1501 aacaacgagg gttcggtttg gcgccgaacc gttacgacta gcgcgtactt tgacccgcgg
     1561 cgagggcggg gtccagcacg cagagttggt cgaagacgcg ctgcaactcg gcgacgtcgg
     1621 cggggacctt gctggcgcgc aagcacgtca cgagcgccga gaggatctgt gagacgaggc
     1681 gtggctccca gtccgcgaga cgtcgctcgt gggcctcacg ctcgagcgcg gcgagctgcg
     1741 cgcgagcgac ggggacgagg ccagcctcga gggtcaggag cgcgagcgcg gtgcgctttt
     1801 cgaagcggtc gcgcgggctc gctgcggtgt cggcgacgag ctgcagctgg gcgatcgcgc
     1861 cggcgcgatc gccggtggcg aggcggcggc gggcgctggc gacctcgtcg ctggcctgtc
     1921 ccgaaggacc tggcgcagag gacaggtttg gtgggggctg gggacctggc gtcggggaca
     1981 ggttgggggg gcgcggtgac ggtgggggcg gggcgaccgg cggcgcgggc atcggagccc
     2041 acgcgatcgg gtcgggggat gcggcggcgc cgtggaggtt ctcggcggcg aggaaggcga
     2101 gggtctcgct gtcggcgagc ggggtgccgt cgcgggcttg cagcgtcggc agggtcggca
     2161 tgcgacgcag cagcgcggtg acctcggcga ggaccgcggt gcgggcgagc ttgtggtcga
     2221 gcgccccgag ggcggctgcg ctgaagcgct ggaggtcgag ggcgaagcgc tgcggggcct
     2281 gggcgagcgc ggactcgctg gcgtcgagca gctcggccca gcgtcctgcg cgggccatcg
     2341 cctcgcactg cgagcgggtg gcgggcggca gtggttcgac gggggcgcgg ccctgggcgt
     2401 cggcgaccgg ctcactggcg cgcagccaga tcgcggtgcg cagcaggcgg taggcgagcg
     2461 ggtcggcggg ctgctgccgg cgcaggccgc tggcgatttt tgaaaagtgc tgggtggcgg
     2521 cgccgaggga ttttaaaagg tcgtcgacgc tggcgccggc cttcgggggc tcgacgctgt
     2581 ccgaagggac aggtgccgga ggcgcgggct tcgggggcgg tggcttcggg ggctcggcct
     2641 ttggtctctc cggcgcggcg gcgaccggcg ggggctcggg ctcggcgggc ttggcctcgg
     2701 ggatcgtcgc cagcaggcgg gtgagcgcgt cgcggacggt gcggttcgga gggggcgggc
     2761 tgtagcgggt ctgggcggtc gtgtgcagct gctcgagcag cgcggcgcag gctttcaaaa
     2821 tgccggcctc gtcgggctcg gcggtcgcgt gggcgccgag ccttgtgccg atgtgctgat
     2881 aaaaattgtc gagggcgttc tcgcgggcct tgcggcgggc gaccggcggg aacatgtcct
     2941 cccagtagtc cctgagcagg ccatccaaca atgcgaggcc gtcggcgagg ccgcgcaggc
     3001 cgtcggtctc gaaggcggcg ccggcccaga aggtggcgat gacgaggtct tttgtgacgc
     3061 tcgtcagcag gccgcgcgag gcctcctcga tcacgggcca gttgcggcgc gcgtactcgt
     3121 ccttgagcat ctcgtcgcgc agcaattcgg cgcgcgggtc ctcgcgcgcg tccttgcctg
     3181 cgcgcgcgtc cccggggacg ggtgcgagca acgcctgcgc gctggtgcgt gcgcgggcga
     3241 cctcggggct gtccggggac tgtgccatgg cttacttgcc ggcctgcttt tccactttgc
     3301 cgacgagcga cagggtgaag gcggcgccgt tgtacttgaa gtgcgggcgc agctggaggc
     3361 ggcaggtgta gcgaccgggc tcaccggcga cgtcctcgac ggtgatcttg gcccagcgca
     3421 ggggcttgct ggcgcgggtc ttcgagtcgg tcgtctccat gttgttgacg tagttgccga
     3481 tccacttgtt gagctcggct tccagctgac tgcggctcat gtcgctgccc aggagctcgc
     3541 gctggcggat cttcaggtaa tgcgccatgc ggctgacgat gaacatgtac ggcagcttgg
     3601 cgccgagctc gaggctctgc tggagctcga cgtcgttggt gcgcgggacc ttctgcaccg
     3661 agttggcgga gaagaagcag ccgcggtcgc tctccttctg atgcacgagc gagatgaagc
     3721 cgttgttggc gagctcgaac tcgcggcggt ccgagatctg gacctcgatc ggcatcttgt
     3781 ggtgctgttt tcccaaaacc tcgtactcgt ggatcgggag gtcctcgacg gcgccgccgg
     3841 agttggggcc gatgatgttc gggcaccagc ggtacttggc gaacgaggcg gcgacgcgcg
     3901 aggtgaaggc gatcgagctg tggccccaca ggtagccgcc gtgctcgccg ccggtgtcct
     3961 cggtgaagtt gaagctggtg acgcggcggt cgttggcgct gtagggcgcg cgcagcatga
     4021 agcgcggcag gcacaggccg acgttgcgcg cgtcctcgga ctcacgaaag ctgcgccagc
     4081 gggcgtactg cggcatctcg aacagcgagt ccatgtcgcg catcttggtg agcacctcga
     4141 aggaggtctc gcccttgccg aagaactcgg tgctggcgtt ggcgatgaag ggcgcgtggg
     4201 ccatcgtcga gacggcggcg cagttgcgca gcaggtcgat gtcgttgccg ctggggccga
     4261 agtcgaagtt gctgcacacc aggccgtagg gcttgccgcc gtagacgccg atgccatcgg
     4321 tgtagacgcg acgaaacagg gtgctcttct cgaccttgcc ggcgtcctcg aagtcgttga
     4381 gcaggtcgtt cttggtgcag ttgaggacgg cgaggcggat gttctgcgtg aagtcgac
//
LOCUS       DASWNJ010000002         1847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_565123, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000002 DASWNJ010000000
VERSION     DASWNJ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565123"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1820)
                     /gene="gspE"
                     /locus_tag="VGB85_00020"
     CDS             complement(<1..1820)
                     /gene="gspE"
                     /locus_tag="VGB85_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HEY0132417.1"
                     /translation="MNTQLTQLHTHDVPVLAPVGVRPQARPIGELLIEMRALTAEALA
                     EGLSAQHLENERIGETLVRLKHCGEWDVCRALAQQFGLQARESIEINEIEDELVERLP
                     IQYARSNTVLPYRLDRQIGLLRVFAADPLRLLDLDDLCQVYGTQDLEITLMPRATVLD
                     LINRVYARRTKDVDLEKKDEEYAEEDEDILHASAEDAPIIRFVNSLIFNASKEKASDI
                     HIEPGDKEIMVRNRVDGVLHEVKRAPKAHLPSIIARVKIMAGLNIAEKRLPQDGRIRR
                     KIAGKEVDMRVATVPTAHGERVTIRLLDKSAVSLSLDSIGIAPDHLRLIRDVIARPHG
                     IFLVTGPTGSGKTTTLYSALSEINTPDLNILTVEDPVEYQLAGISQVQVQSKINLTFA
                     AGLRSFLRHDPDVIMVGEIRDVETAEIAIQASLTGHLVLSTIHTNDAATGITRLVDMG
                     VQPFLVASSLVALQAQRLVRRVCPHCCEPYKPTSAEIEDVGIDAKAFFAGEQALKVPV
                     RNEDGSIIPLAAPTGRKLPPLGHLYKAAGCEKCHASGYSGRTGLYEVLTVTEEVRRLA
                     IRNADSGQIKAAALAQGMRTLRDDGAHKVLCGITTLDEVVR"
BASE COUNT          265 a          580 c          672 g          330 t
ORIGIN      
        1 cgcacaacct cgtcgagggt agtgatgccg cacaggacct tgtgcgcgcc gtcgtcacgc
       61 agcgtgcgca tgccctgagc cagcgccgcg gccttgatct gaccggagtc ggcgttgcgg
      121 atcgccaggc gacggacctc ctcggtgacg gtgaggacct cgtagaggcc ggttcgcccg
      181 ctgtagccgc tcgcatggca cttttcacag ccggcggcct tgtacaggtg gcccagtggc
      241 ggcagcttgc ggccggtcgg cgccgccagc gggatgatgc tgccgtcctc gttgcgcacc
      301 gggaccttga gcgcctgctc gccggcgaag aacgccttgg cgtcgatgcc gacatcctcg
      361 atctcggcgg aggtcggctt gtagggctcg cagcagtgcg ggcacacgcg acgtaccagg
      421 cgctgggcct gcaaggcgac gagcgaggag gcgacgagga acggctgcac gcccatgtcg
      481 acgaggcgag tgatgccggt cgcggcgtcg ttggtgtgga tcgtcgagag cacgaggtgg
      541 ccggtcagcg acgcctggat cgcgatctcg gcggtctcga cgtcgcggat ctcgcctacc
      601 atgatgacgt cggggtcgtg acgtaggaag gagcgcagac ctgccgcaaa ggtcaggttg
      661 atcttgctct gcacctggac ctgggagatg ccggcgagct gatactcgac cgggtcctcg
      721 acggtcagga tgttgaggtc cggggtgttg atctccgaca gcgccgagta gagcgtcgtc
      781 gtcttgcccg agccggtcgg accggtgacg aggaagatgc cgtgcgggcg agcgatgacg
      841 tcccggatca ggcgcaggtg gtccggggcg atgccgatgc tgtcgaggct gaggctgacc
      901 gccgacttgt cgaggaggcg gatcgtgacg cgctcgccgt gcgccgtcgg cacggtggcg
      961 acgcgcatgt cgacctcctt gccggcgatc ttgcggcgga tacggccgtc ctgcggcagg
     1021 cgcttctcgg cgatgttgag gccggccatg atcttgacgc gggcgatgat gctcgggagg
     1081 tgggccttgg gcgcccgctt gacctcgtgc agcacgccgt cgacccggtt gcggaccatg
     1141 atctccttgt cgccgggctc gatgtggatg tcgctcgcct tctccttgga cgcgttgaag
     1201 atcaggctgt tgacgaagcg gatgatcggc gcgtcctcag ccgaggcgtg caggatgtcc
     1261 tcgtcctcct cggcgtactc ctcgtccttc ttctcgaggt cgacgtcctt ggtccgccgc
     1321 gcgtacacgc ggttgatcag gtcgagcacc gtcgctctgg gcatcagggt gatctcgagg
     1381 tcctgcgtgc cgtacacctg gcacaggtcg tcgaggtcga gcaggcgcag cgggtcggcc
     1441 gcgaacacgc gcagcaggcc gatctgacgg tcgaggcggt acggcagcac ggtgttgctg
     1501 cgcgcgtact ggatcggcag gcgctcgacc agctcgtcct cgatctcgtt gatctcgatg
     1561 ctctcgcgcg cctggagccc gaactgctgg gcgagcgcgc ggcacacgtc ccactcgccg
     1621 cagtgcttga ggcggaccag cgtctcgccg atgcgctcgt tctcgaggtg ctgggccgag
     1681 aggccctcgg cgagcgcctc ggcggtcagg gcccgcatct cgatcaacag ctcgccgatc
     1741 ggacgcgcct gcggacggac accgaccgga gcgagcacag ggacgtcatg cgtgtggagc
     1801 tgcgtgagct gcgtgttcat ggtgacctcg gaacctctaa ccatagc
//
LOCUS       DASWNJ010000003        19587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_494493, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000003 DASWNJ010000000
VERSION     DASWNJ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 19587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19587
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494493"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /gene="tsaD"
                     /locus_tag="VGB85_00025"
     CDS             complement(<1..906)
                     /gene="tsaD"
                     /locus_tag="VGB85_00025"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY0132418.1"
                     /translation="MPTAAKILAIESSCDETACAVVEGLHVRGSIIASQVEIHAAYGG
                     VVPELASRHHLTAAVPVLRAAVEAAGIDFSELDGLAVTEGPGLVGALLVGVQLAHGIA
                     LATGLPLHGIHHMEGHLLSPFLGDAQMAPREFVPHLALSVSGGHSELVLVEGLGNYRV
                     LGCTRDDAAGEAFDKVAKLLGLGYPGGPVIDRLAAEGNPTAIHFPRAMLADRERFEFS
                     FSGLKTAVSVHIDQHGQPASRAALADLCASFQAAVVEVLVAKARLARRKFGCKAMTIV
                     GGVSANRGLREAMAVAAAAEGFELWT"
     gene            complement(954..3299)
                     /locus_tag="VGB85_00030"
     CDS             complement(954..3299)
                     /locus_tag="VGB85_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEY0132419.1"
                     /translation="MARQNLLLVDGDARHLRVLEVSLRRAGFSITSAESSLQGLQYLE
                     TAEPDLIISDTHLADGDGFDFCRAVKQSPRWSGIPFIFLTSATELEDKVRGLELGVED
                     YLTKPIYVKEVTTRVKMLLQRKQQERLGRKDARTKFSGQLADMAIVDLLQTIEISRKS
                     GTIEFETDLGHATVWFREGKVVDAQMGRMQAAAAIYRLLGINEGSFEVEFRNINRVGV
                     IRESTQALLMEGMRRVDEWVRLLEGLPPLDHTLTVDIRLLAARLEPPSTMQADLLRRF
                     DGRRTIIEIIDDSGIDDLLALEVVSGLYFEGLLSDEATVPEEDEHAGAPSVGNLDAWD
                     PAVTRAGVPPLPSVPMEPATAPTSAADLPPLPSFPLPFPNLSASIDHDDVLVGGIPDD
                     VRDPQESEEGRIMAVTGARRRRTDKVTAPAAPTVRRLEAVDGHVALPMGMARAEAFGE
                     LQSRPEENRPDLRTHSPSSSIIVRNRDSRDIRSSSSGASGSLASAPALAANSAVTAAP
                     PLADASDTWPHEGPPQLLNESREDMSRLSALVAALNPSPSEVGPLPDTLKHNEPRPTR
                     SSANRWAWATLAAAAIVMMVVMQRWDAEGSVPVATKPMVVQPPVPAKPVEMFVASAKP
                     EPTPEPEPEPAKVEPTPEPEPAKVEAPPGVPPVVDEVVLKREVEKASKLYMSGRLREA
                     LVAAEAALQQDPHHAPALLVKANVLIERKDFETAKLAAEDAITSDRMMADAWLALGVI
                     EQERGATDASIAAYERFLELAPKSRYAGSIRTQLKQMRPGP"
     gene            3888..5006
                     /locus_tag="VGB85_00035"
     CDS             3888..5006
                     /locus_tag="VGB85_00035"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY0132420.1"
                     /translation="MRPEPDASDDLDAWWRRLPQRPVTASLLVLCVLAYTATMVAVLV
                     SAPDPWATATQSIWSLEGSEQILIQLGALDLTRVWVDGEWWRVVTSGLLHGSLIHLVL
                     NMMALGSIGDWVEHAWGSWRSALLFVLTSVAGCLASLLWCESRMVVGASAGVLGQAGA
                     LWVARQFGSPRLQAALAPVSVLSLGILILLCLFLGTVIPAIAQAGHVGGLGMGLILGA
                     LMARSWPRWVHALGGLLVAAILLGLAALARAPTWNPQHHALLGFRAGSEGDTTTATYH
                     FKNSLELDPDNTALQNAIAYQFALDGIELEQAEALIRRALALEPTNANYLDTLGWILC
                     RKGDPELAEPLLRAAVFLADESVPEIESHIGFCASSAI"
     gene            complement(5267..5902)
                     /locus_tag="VGB85_00040"
     CDS             complement(5267..5902)
                     /locus_tag="VGB85_00040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HEY0132421.1"
                     /translation="MSAGNLQVLLEPHVSAIGLKRDVPWAKLEELGRQWLRFGRAMNL
                     SGAKDLQGLAEQIAEGLEVVALAEKLGCTEGDRWVDVGSGAGFPGLIVASCLPVEIWL
                     VEPRERRAAFLELMLAQVRAKGRVVRGRLEDGGVREERGGLVETSGFRWASARAVFSP
                     EVWVQRSQVLLAEQGVTIVHLHRGDADPLGLVAAASVDGERWSVRGYRRFT"
     gene            5946..7100
                     /locus_tag="VGB85_00045"
     CDS             5946..7100
                     /locus_tag="VGB85_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0132422.1"
                     /translation="MPTPLDRRLQFKLTEQARAGLTRTLPEISERDGVRYRLHGRPVT
                     GFCSNDYLGLADDPHLAVSTTTTGATASRLVCGDLPEHRSLETRLARLAGAEDAVLFP
                     SGFQLNVGVLPALIQPGDQVFSDALNHASLIDGLRLARAELRVLEHGTAPNPAGLSAN
                     SASPDPLRWWVTEALFSMDGDYADPAAMQQHLAHDGCLYLDEAHSFGLFTRDGHATGL
                     AGMHGFLPTALVGTLGKTFGCAGAFVAASSVACRWIRGAARSFVFSTGVSPVIAAQLH
                     RAIDLVDGLEGERRRTRLWANVDQLARRLGVPKHRSPIFRVLLGSNEAALALSAQLVE
                     RGWHAQAIRPPTVPRGTSRLRLTVTATHEPAQIDAFADDLHALLPDHRIT"
     gene            complement(7516..8829)
                     /locus_tag="VGB85_00050"
     CDS             complement(7516..8829)
                     /locus_tag="VGB85_00050"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA modification GTPase"
                     /protein_id="HEY0132423.1"
                     /translation="MASASAAGEQPTIVGVATGTPDGGVAIVRLSGSRAMGIARALCG
                     ELPTARHLARRRVKLDERGVVEDALVVVMPGPGSFTGEDVVELHVHAGARNVASVVTA
                     VLGHGAVAADAGEFSRRAFEHGRLSLEQAEGIAALIAARTDAALEQARRLVAGELGRE
                     VSRLAAEIHALRCEVEANLDFSEDVEAGDLRRWSGEISGFAATMQAWIGRFEAGRRSR
                     ERARVVLAGPPNAGKSAVFNALLGRPRAIVSPVPGTTRDYVEAELMLGSHEVVLVDTA
                     GLREAMDLIEDAGISRSRDQIGGADLVVWIEAADGAGAEAVAAGIRVENKRDLGCRRP
                     DWVGASAAAGEVGELRTALHAWVQSRTEHPWIGLARHRDRVADSGEALEDAAAVLAKD
                     GPLEIAAFQLGVAEQRLAEVTGRGTIGPISEDVLKDIFSRFCIGK"
     gene            complement(8850..9410)
                     /locus_tag="VGB85_00055"
     CDS             complement(8850..9410)
                     /locus_tag="VGB85_00055"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEY0132424.1"
                     /translation="MADSKQAAPPAETPANADQNYGKAVPVVRSFLESVLGLLGVEVE
                     VDIDIEEDGLHCDLNTSEEDGGLLIGRHGATLDALQYLTLRVLQAEGFDRMRITLDVG
                     GYRTRREKSLKELAQGVGQKVLTSGRPYHFDPMSAMERRVVHMTLMEMEGLRTESEGE
                     GRFRHVVVFPARDGDGRRSENDDARA"
     gene            complement(9428..11050)
                     /gene="yidC"
                     /locus_tag="VGB85_00060"
     CDS             complement(9428..11050)
                     /gene="yidC"
                     /locus_tag="VGB85_00060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEY0132425.1"
                     /translation="MDFQRRTLLAIVVSVLIWIVYASFFAPPMPEPKAATASTSTADA
                     AEVDAPRGADAKADAEAAPDSKPHNEAPAETHRLKTDLIALDVGNVGGIIRRTELLSK
                     QFVRADGIGDDFLHLGGADSLAVTFDANETDFRMPRNAGWEVVGKTDTTWSLRYKDLD
                     VEILGDLKVAGGYEAQLQVKVLNRSSKRQAHRMLLKSQYGLPKEASSYDIHRGLCATA
                     DDVEEFDSSDVSESSGTIRGQIRWAALDSKYFVSAVVPTHPVQMCEVKLSDDGAMLVS
                     TIGSNTVTLEPNESRTYEFGLYLGTKEMERLSSFAVVPGVHLEDAVDWGWFGGLTRFL
                     GEKMLWLLRLFYNLTGIWGVAIILLTVVVKVVTLPLTFKQMRSMKKMREVQPELEALK
                     KKYADDRVRQAQEMQALFKRAGVNPLAGCLPMLIQLPIWIALYAMLNTAVELYREPFL
                     WLPDLTQQDPYYILPLAMGALMYFQMKIQPSGVDNEQAKMMAWMMPIIFTVMMLFLPS
                     GLCVYIFANVLLSLVQTVIQLRPTKAAVATKS"
     gene            complement(11054..11329)
                     /gene="yidD"
                     /locus_tag="VGB85_00065"
     CDS             complement(11054..11329)
                     /gene="yidD"
                     /locus_tag="VGB85_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEY0132426.1"
                     /translation="MIASIRVYQRVLSPLLGPCCRFTPSCSHYACECIRDHGALRGGW
                     LTLRRLLRCHPWHPGGLDLPPALPEAKTSKQRSSPSVMDAPSSLENC"
     gene            complement(11338..11715)
                     /gene="rnpA"
                     /locus_tag="VGB85_00070"
     CDS             complement(11338..11715)
                     /gene="rnpA"
                     /locus_tag="VGB85_00070"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEY0132427.1"
                     /translation="MKPEGFPAAIRVRKRREFLAIQGAGAKHHLRHFLVFVVPPASRP
                     VEERSDVVRLGITVTRKVGCAVVRNRIKRYVREAFRRKRGSFTPGYDMVWVAKQSAAS
                     VGYDEVVAAMELLGKRPPLGASC"
     gene            complement(11712..11864)
                     /gene="rpmH"
                     /locus_tag="VGB85_00075"
     CDS             complement(11712..11864)
                     /gene="rpmH"
                     /locus_tag="VGB85_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEY0132428.1"
                     /translation="MSKRTYQPHNLSRARTHGYRARMATRGGRKVLAARRRRGRKRLS
                     VSIGGK"
     gene            12638..14074
                     /gene="dnaA"
                     /locus_tag="VGB85_00080"
     CDS             12638..14074
                     /gene="dnaA"
                     /locus_tag="VGB85_00080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEY0132429.1"
                     /translation="MSDIWSEALQGLQPRLGSQTYDLWLRPIALRRVNGTRLHLTAPN
                     RFMKEWFENHYLGVVLDELRARTSQTYDVEIEVTADAPAAPPATAPAAVPTEPVRPAS
                     PPPVPALVAAPPPQLSGRYRFPNFIKGAGNELAASAAWAAANEPGTRFNPLFIYGGVG
                     LGKTHLLHAIGHHIHATRPEQRIVYQSAERFMNEFIAAVRFNQFDEFRSRYRDQCDVL
                     LIDDIQFIAGRDRTMDEFFHVFNVLYEAEKQIVVTADRMPSEMQGMEDRLISRLNWGL
                     VADIKPPDLETRVAILRQKAEHDHIVIADEVTLHLANIVRSNVRELEGSLVRVSTLAS
                     LKNVPITCEFVSELLGAPAGSAQPPPVSIEQVQKVVCAHFSVRVSDLKGPRRHQGISR
                     PRMIAMYLCRQLTSASFPEIGLRFGGKDHSTVINACKRIESIQAEDRELRATVDTLRR
                     QLTGAESTSTTVHTGSGGATIAAPGAVL"
     gene            14458..15561
                     /gene="dnaN"
                     /locus_tag="VGB85_00085"
     CDS             14458..15561
                     /gene="dnaN"
                     /locus_tag="VGB85_00085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEY0132430.1"
                     /translation="MEFEIDQSKFSHALAQAQTVADKRSNNMPILANVLLRATKDNKL
                     ICSATDMMISVTETIPVEVKTPGALTLGVRYLHNVVRTLPSKPLRIRGLENHWAQLSV
                     GRSEFKLMGQAPGDFPELPDTKGIKFTRLPGHTVADLIQKTQFSVSTDEARVNLNGVL
                     FESSGELGTMVSTDGHRLTKLSLPLTGPKLDRGIIIPRKGMLEIKRVLDRVSGEVGLA
                     VDGQHLYLQAEELLLTVKLNNVVFPPYEQVIPREFRRRVTALRDEMIDVLKRAEVMAP
                     EKTATVRLELDKGSLKLTADNPDLGVAHQEIEIEYSGEPLVAGFNARYLIEVLEAMDT
                     KQVHLDFQGELDPCVLRPVDGPDYLGVVMPMRI"
     gene            15565..16704
                     /locus_tag="VGB85_00090"
     CDS             15565..16704
                     /locus_tag="VGB85_00090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEY0132431.1"
                     /translation="MQLEHLSIREFRNLGAGEVSFGPRFTVLHGHNGAGKTNVLEALY
                     LVGTLRSFRTADLGPLVRHGQPEARVEVRGLDPRVGISSKLVVQLSRTERATRRLAQA
                     DGKTVRSGLDFYGRLRAILFTPEDLGVLRGSPTGRRQFIDRVLFARERTHISDTQDYE
                     KLLRSRNRVLREEEGTPRQRDDLLASYEVGLAEVGGRIWTRRERMLSDLHARFTAAFA
                     GIHGATLPATMTYVSKVGALGGRTAQDVLGEALKERRRHDIVRGTTTVGPHRDDFDMQ
                     LGEQSASTYASQGQARALILAFKIAELQAARELTGEAPLLLLDDVSSELDPERSAQLF
                     AALVREAGQCVLTTTAPHFIGLEAGEDCRYIEVKGGRIEPVPTPP"
     gene            16869..19376
                     /gene="gyrB"
                     /locus_tag="VGB85_00095"
     CDS             16869..19376
                     /gene="gyrB"
                     /locus_tag="VGB85_00095"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEY0132432.1"
                     /translation="MSEADKTQAADEYNADSISVLEGLEAVRKRPGMYIGDTSDGSGL
                     HKMVFEVVDNSIDEALGGHCDTIEVIIHPDNSVSVEDNGRGIPTGPMEASGKTMDAAI
                     VILTVLHAGGKFNNNSYKVSGGLHGVGVSVVNALSDWLQLDIWREGKAHAVRFERGNP
                     VTEVVHVGPADRRGTRVHFHADPLVFTITEIDFEVLANRFRELSYLNSNVKMTLRDLR
                     TGKERVFDGEGGVPSFVKLLAQNKEAIGDIVVFNKDIEFEHEGRTAVLGVQIAMQWTD
                     VYQETILCYTNNIANRDGGTHLTGFRTALTKVINKYASESGALKGHKDTLAGEEVREG
                     IVGVVSIRHPDPKFSSQIKDKLVSGEVTSLVSTVVTEELSRYLEENPRSARLIVEKAL
                     LAARARAAARKARETITRKGILDGLSLPGKLADCQERNPEHAELFLVEGDSAGGSAKQ
                     GRDRKTQAILPLRGKILNVEKARIDKMLSSQEIITLITALGTGIGLDNYDIGKLRYHT
                     IVIMTDADVDGSHIRTLLLTLFYRHFPDIIERGHLYIAQPPLYKVKSGKAESYLKNDA
                     ALEDFIISNAVESMRLSIGGHFVMPEVLGEIARQGLRYRHLMESMAREHRAPVVDHML
                     NDLHQGAIKPDVFSDEPALADFGSRMQAAVAQQMPLARVSFEVVDESVDPAREAARAT
                     AGISIERRAIRLQIIADGVAQYEVVDHELLNSYPVQEMLKIRHYMDTLGLPPYRLYRG
                     ENVVAEPARLVDVVQHVGELGRSGLQIQRYKGLGEMNPEQLWETTMDITRRALLKVRV
                     GDNVDADNVFSVLMGDNVEPRRDFIVNNALNARNLDI"
BASE COUNT         3183 a         7214 c         6084 g         3106 t
ORIGIN      
        1 cgtccacaat tcaaaaccct cggccgcggc cgcgacggcc atcgcctcac gcagcccgcg
       61 attggccgac acgccgccga cgatcgtcat cgccttgcag ccgaacttgc gccgcgccag
      121 cctggccttg gcgacgagca cttcgacgac cgccgcctgg aacgacgcgc acaggtccgc
      181 cagcgccgcg cgcgaggccg gctgtccgtg ttgatcgatg tgcacggaca ccgccgtctt
      241 cagccccgag aacgagaact cgaagcgctc gcggtccgcg agcatcgccc gcggaaaatg
      301 aattgcggtc gggttcccct cggccgcgag gcgatcgatc accggcccgc ccggatatcc
      361 gagcccaagt aacttcgcga ccttgtcaaa agcctcgccg gccgcgtcgt cgcgcgtgca
      421 cccgagcacg cgatagttcc cgagcccctc gacgagcacc aattcgctgt gcccaccgga
      481 caccgacagc gccaggtgcg gcacgaattc ccgcggggcc atctgcgcat caccaaggaa
      541 cggcgagagc aggtgccctt ccatgtgatg gatcccgtgc agcggcagtc cggtcgccag
      601 cgcgatccca tgcgcgagct ggaccccgac gagcagcgca ccgacgagcc cgggcccctc
      661 ggtcacggcg agcccgtcta gctcggaaaa atcaatgcct gccgcctcga ccgccgcgcg
      721 cagcaccggc accgccgcgg tcaggtgatg tcgactcgcc agctccggca ccaccccgcc
      781 gtaggccgcg tggatctcga cctgcgacgc gatgatgctc ccgcgcacgt gcaggccctc
      841 gacgactgcg caggcggtct cgtcgcacga cgactcgatg gcgaggatct tcgcggccgt
      901 tggcatcgtt ccgggctggc gtagcgcgct tcgggggccg gttcaagcgc gccctacggg
      961 cccggacgca tctgcttgag ctgcgtgcgg atgctcccgg cgtaccgcga tttgggcgcc
     1021 agctcgagga agcgttcata cgcggcgatc gacgcatcgg tcgcgccgcg ctcctgctcg
     1081 atgaccccga gcgccagcca ggcgtccgcc atcatccggt cgctggtgat cgcgtcctcg
     1141 gccgccagct tcgccgtctc gaagtccttg cgctcgatca gcacgttcgc cttgaccagc
     1201 agcgccggcg cgtggtgcgg atcttgctgc agcgccgcct cggcggccac cagcgcctct
     1261 ctcagacggc ccgacatgta cagcttcgag gccttctcca cctcgcgctt gagcacgacc
     1321 tcatcgacga ccggcggcac cccgggcggc gcctcgacct tcgccggctc gggttcaggc
     1381 gtgggctcga ccttcgccgg ctcgggctcg ggctcgggcg tcggctcggg cttcgcggac
     1441 gcgacgaaca tctcgaccgg cttcgcgggc accggaggtt ggacgaccat gggtttcgtg
     1501 gccaccggca cgctgccctc ggcgtcccac cgctgcatca cgaccatcat cacgatcgca
     1561 gccgccgcca gcgtcgccca cgcccaccgg ttcgcggacg aacgcgtcgg gcgcggctcg
     1621 ttgtgcttga gcgtgtcggg cagcgggccg acctcgctcg gagaaggatt gagcgctgcg
     1681 accagggcgc tgaggcggct catatcctct cggctctcgt tgagcagctg cggcggtccc
     1741 tcgtgcggcc atgtgtcgga ggcgtcggcg agcggcggcg cggctgtcac ggcgctgttg
     1801 gccgccagcg ccggcgccga ggccagtgag ccggacgcgc ccgacgacga cgagcggatg
     1861 tcgcggctgt cccggttgcg cacgatgatc gacgacgaag gcgagtgcgt gcgcaggtcg
     1921 gggcgattct cttccggtcg cgactgcagc tcgccgaacg cctctgcacg ggccatcccc
     1981 attggcaggg cgacgtggcc gtcgaccgcc tcgagtcggc gcacggttgg cgctgcgggg
     2041 gccgtcacct tgtcggtgcg acgccggcga gcgcccgtca cggccatgat ccttccctcc
     2101 tcggactcct gcgggtcgcg cacgtcgtcc ggaataccac cgaccagcac gtcatcgtga
     2161 tcgatgctcg cgctgaggtt gggaaagggc agcgggaagc tcggcagcgg cggcaggtcg
     2221 gccgcgctgg tcggcgctgt tgctggctcc atggggaccg acggcagcgg tggcaccccg
     2281 gcccgcgtca cggcggggtc ccaggcgtcg aggttcccga ccgacggcgc tccggcgtgc
     2341 tcgtcctcct cgggcaccgt cgcctcgtcg gacagcaggc cctcgaagta cagcccgctg
     2401 accacctcga gcgcgagcag gtcgtcgatc ccgctgtcgt cgatgatctc gatgatcgtc
     2461 cggcgtccgt cgaagcggcg cagcagatcc gcctgcatcg tgctgggcgg ctcgaggcgg
     2521 gccgcgagca ggcggatgtc gaccgtcagg gtgtggtcga gcggtgggag gccctcgagc
     2581 aggcgcaccc actcgtcgac acgccgcatg ccctccatca acagcgcctg cgtcgactcc
     2641 cggatcacgc cgacccggtt gatgttgcga aactcgacct cgaagctgcc ctcgttgatc
     2701 ccgagcaggc ggtagatcgc cgctgctgcc tgcatgcggc ccatctgggc gtcgaccacc
     2761 ttgccctcgc ggaaccacac cgtcgcgtgg ccgaggtccg tctcgaactc gatcgtcccg
     2821 ctcttgcgcg agatctcgat cgtctgcagg aggtcgacga tcgccatgtc ggcgagctgt
     2881 ccgctgaact tggtgcgggc gtccttgcga ccgaggcgct cctgctgctt gcgttgcagc
     2941 agcatcttca cccgcgtcgt cacctccttc acgtagatcg gcttggtcag gtagtcctcg
     3001 acgccgagct cgagcccacg gaccttgtcc tccagctccg tcgcgctcgt caggaagatg
     3061 aacgggatgc cggaccagcg cgggctttgc ttcaccgccc ggcaaaaatc gaagccgtcg
     3121 ccgtcggcca ggtgcgtgtc ggagatgatc aggtccggct cggccgtctc gaggtactgc
     3181 agcccctgca gcgagctctc cgcgctcgtg atgctaaagc cggctcgacg caggctgacc
     3241 tccagcacgc gcaggtgccg ggcatccccg tcgacgagca gcaggttctg gcgagccatc
     3301 cggttcccta ccgtccccgg cccgcgtcgg gacccggggc agtcaccgcc cgcagcttgc
     3361 gtctccgtct gcctgtcatc acggtcgaac gcacccggac agcataacga taatcgtgcg
     3421 tgccaccggc tcgcaagcca gccaaaagac cgccgggcgg cgcaaaagcg cccgttgtat
     3481 gtcctgaagg acaggtcagc acggcgcgag ccgacgcggg cggcgcagga accgaggggt
     3541 gcggagtatc gccgcgcgca ccggcctgag cccgacgcct ggcgcctccg cagcgtccgg
     3601 gtctggctcc gtcatctcgg aacgaacttc ccgttgcctg gcccccgctt gcagcgttcg
     3661 gttgcgaagg gggtcgggca tgcaggtccg tgcgagtcga gcccggcgcg gacgacaact
     3721 tcgacgacct caggcttcgg tctcaaaagc acggaagtgc tggccgtcgt cggccgaacg
     3781 aaccgagccc cacgcgagcg actcgacgcc cccgcttccg cggcatcggt tcggcaaaaa
     3841 ctacggacgt gcggggtctg gctccgtcat ctgctagggt ctcggccgtg cgacccgagc
     3901 ccgacgcgag cgacgacctc gacgcctggt ggcggcgcct cccgcagcgc ccggtcacgg
     3961 cgagcctgct ggtcctgtgc gtacttgcct atacggcgac catggtggcg gtgctggtca
     4021 gcgcgcccga cccctgggcc acggcgacgc aaagcatttg gagtctcgaa ggcagcgagc
     4081 agatcctcat ccagctcggt gccctcgacc tcacccgcgt ctgggtcgac ggcgagtggt
     4141 ggcgcgtcgt caccagcggc ctcctgcacg gctcgctgat ccacctcgtc ctcaacatga
     4201 tggcgctcgg gtcgatcggc gactgggtcg agcacgcgtg gggttcctgg cgcagcgcgc
     4261 tcctgttcgt gctgaccagc gtcgccggct gcctcgcctc gctgttgtgg tgtgagtcgc
     4321 ggatggtcgt cggcgcgtcg gccggagtgc tcggacaggc cggcgcgctg tgggtcgctc
     4381 gtcagttcgg cagcccgcga cttcaggccg cgctggcccc ggtctcggtg ctcagcctcg
     4441 gcatcctcat cctcttatgt ctgttcctcg gcaccgtcat cccggcgatc gcccaggccg
     4501 gtcatgtcgg gggcctcggc atgggtctga tcctcggcgc cctgatggcc cggagctggc
     4561 cgcggtgggt tcatgccctc ggtggcctgc tcgtcgcggc gatcctgctg ggcctggccg
     4621 cactggcccg cgccccgacc tggaacccgc agcaccacgc gctgctcggc tttcgtgctg
     4681 gcagcgaggg cgacaccacc actgccacct accacttcaa aaattctctc gagctcgacc
     4741 cggacaacac ggccctgcag aacgcgatcg cctatcagtt cgcgctcgat ggcatcgagc
     4801 tcgagcaggc cgaggcgctg atccgtcgcg ctcttgcgct cgaaccaacc aacgcgaact
     4861 acctcgacac gctcggctgg atcctgtgtc gcaaaggcga tcccgagctg gccgagcctc
     4921 tcctgcgcgc cgcggtgttc ctcgcagacg aatccgtccc cgaaatcgag agccatatcg
     4981 gtttttgcgc gagcagcgcg atctaggtag tgttccacgt gaaacgtcgg cgagtcgccg
     5041 tcacaacgtt ccacgtgaaa cgtctgtagc ccggtgcctc ggccgtgcca caacgtttca
     5101 catgaacgcc gatagcctgg actatccggt gccctcggcc gcgccacagc gtttcacgtg
     5161 aaacgtcgat ggcccggact ctccggtgcc ccaggccgtg ccccaacgtt tcacgtgaaa
     5221 cgtcaacgcc ccggactatc cggttccccg gccgcgccac aacgtttcac gtgaaacgtc
     5281 tgtagccccg gaccgaccag cgctcaccgt ccaccgatgc tgccgcaaca agcccaagcg
     5341 ggtctgcatc tccacgatgc aagtgaacga tcgtgactcc ctgctcggcc aacaacacct
     5401 gcgaccgctg cacccagacc tcgggtgaga acacagcccg ggccgacgcc caacgaaacc
     5461 cggacgtctc gaccagccca ccccgctcct cccgaacgcc cccatcctcg agccgccctc
     5521 gcacgacccg ccccttcgcc cggacctgcg ccaacatcag ctccaaaaat gcagcccgtc
     5581 gctctcgcgg ctcgaccagc caaatctcga ccggcaagca actcgccacg atcagccccg
     5641 ggaagccggc gcccgaccca acgtccaccc agcgatcccc ctcggtgcac ccgagcttct
     5701 cggcgagcgc taccacctcg agcccctcgg caatctgctc ggccaacccc tgaaggtcct
     5761 tcgcaccact gaggttcatc gcccgcccga accgcagcca ctgccggccg agttcttcaa
     5821 gcttcgccca cggtacgtcg cgcttgagcc cgatcgccga cacgtgcggc tcgagcaaga
     5881 cctgcaggtt gcccgcggac atgaatccgc agtaagacct gcaccgcccg gctgtcaagc
     5941 gccccatgcc cactcccctc gaccgccgcc tccagttcaa gctgaccgag caagcacgcg
     6001 ccggccttac acgaacgctc cccgagatct ccgagcgtga cggcgtccga tatcgccttc
     6061 acggccgtcc ggtgaccggc ttctgcagca acgattacct cgggctcgcc gacgaccccc
     6121 acctcgcggt ctcgaccacg accaccggcg caaccgcctc acgactcgtc tgcggggatc
     6181 tccccgaaca tcgttccctc gaaacgcgcc tggcccggct tgctggcgca gaggacgctg
     6241 tactgttccc gagcggcttc cagctcaacg tcggcgtcct gcccgccctc atccagcccg
     6301 gcgaccaggt cttcagcgac gcgctcaacc acgccagcct catcgacggc ctgcgtctcg
     6361 cccgcgccga gctccgcgtc ctcgaacacg gtaccgcccc gaaccccgcc gggctctctg
     6421 caaacagcgc gtcgcccgac ccgctccgct ggtgggtgac cgaggccctg ttctcgatgg
     6481 acggcgacta cgccgacccc gctgcgatgc agcaacacct cgcccacgat ggctgcctct
     6541 acctcgacga ggcccacagc ttcggactgt tcacccgcga cggccacgcg accggcctcg
     6601 ccggcatgca cggcttcctc ccgaccgcgc tggtgggcac tctcggcaag accttcggct
     6661 gtgcaggggc cttcgtcgcc gcctcatccg tggcctgccg atggatccgt ggcgccgcgc
     6721 gaagcttcgt gttcagcacc ggcgtctctc cggtgatcgc tgctcaactc catcgggcga
     6781 tcgatctggt cgacggtctc gagggcgaac gccgccgcac tcgtctgtgg gccaacgtcg
     6841 atcagctcgc gcgccgcctc ggcgtcccca agcaccgctc accgatcttc cgcgtgctcc
     6901 tcgggagcaa cgaggccgcc ctggcactga gcgctcagct cgtcgagcgt ggctggcacg
     6961 cgcaggcgat ccgccctccc acggttccac gtggcacatc gcgcctgcgg ctcaccgtga
     7021 ccgccacgca tgagccagcc cagatcgacg ccttcgccga tgacctgcac gcccttctcc
     7081 ccgatcaccg gatcacctga gagccttctc gcaggaactg ctcgtcgagc accgagctcc
     7141 cggcgggctt gcaacggccc aatgactctc tcgagtccac cccggtgtca cgatctcggc
     7201 accgccagca tttgacaccg atcggctcgc tggccgaact gcgtcccctg tcctctgcac
     7261 tcatcgccac gccagcgccc gaaccctctg ctgtctccga caggcccgtc gtcatgcgaa
     7321 cggcgcatgc cttcgctggc tccgctagga cccttgccac accaacgccc gcacacttca
     7381 gctttccccg gcacacgcct gcgtctcttc actgcccccg gaaggcgcag tcgccaggtc
     7441 ctacgtccct ctccgcacca aatcccaaca tcaatcgctc gccggccggt gccagcgcgc
     7501 tacacggccg gcactctact tgccgataca aaaccgcgag aagatgtctt tcaagacatc
     7561 ttctgaaatt ggcccgatcg tgccacgacc ggtcacctcg gcgagccttt gctccgccac
     7621 gccgagctga aacgccgcga tctcgagcgg cccgtccttt gccagcacgg ccgccgcatc
     7681 ctcgagggcc tcgccactgt cggcgacccg atcacgatgc cgggccaggc cgatccacgg
     7741 gtgctcggtc cgactttgca cccaggcatg cagcgcggtc cgcagctcgc cgacctcacc
     7801 cgcggccgcg ctcgccccga cccaatccgg ccgacggcac cctaggtcgc gcttgttctc
     7861 cacgcggatc ccagcggcca cggcctccgc acccgcaccg tccgccgcct cgatccacac
     7921 caccagatcg gccccaccga tctggtcacg actgcgagag atcccggcgt cctcaattag
     7981 atccatcgcc tcacgcaacc ccgcggtgtc gaccaggacg acctcgtgcg atccgagcat
     8041 cagctccgcc tcgacataat cgcgggttgt ccccggcacc ggcgagacga tcgcccgcgg
     8101 gcgtcccagc agcgcgttga acaccgcgct cttgccggcg ttcggcggac ccgcgagcac
     8161 gacgcgagcc cgctcgcgcg agcgacggcc cgcctcgaag cgaccgatcc acgcctgcat
     8221 cgtcgccgcg aatccggaga tctcgccgct ccagcgccgc aggtcgcccg cctcgacatc
     8281 ctcggaaaag tcgaggttcg cctcgacctc gcagcgcagc gcgtggatct cggccgccag
     8341 ccggctcact tctcgcccga gctctccggc caccagcctt cgcgcctgct cgagcgccgc
     8401 gtcggtgcgc gcggcgatca acgctgcgat cccctccgcc tgctcgaggc tcagccgtcc
     8461 gtgctcgaac gcccgccggc tgaactctcc tgcatcggcc gcgaccgcgc catggccgag
     8521 caccgcagtg accacgctcg ccacgttccg ggcgcccgcg tggacatgca gctcgacgac
     8581 atcctcgccg gtgaacgacc ccggcccggg catcaccacc accagcgcat cctccacgac
     8641 accacgctcg tcgagcttca ctcgccggcg cgccagatgc cgcgcggtgg gcagctcgcc
     8701 gcacagcgct cgcgcgatcc ccatggctcg ggagccgctg agccggacga tcgccacgcc
     8761 gccgtccgga gttccggtcg cgacgccgac gatcgtcggc tgttcacccg cggcggacgc
     8821 tgacgccaca gcccacgggt ctcccgggct caggcccggg cgtcgtcgtt ctcggagcga
     8881 cgaccatctc cgtcgcgcgc cgggaacacc accacgtgcc ggaagcgacc ctcgccctcc
     8941 gactcggtgc gcagaccctc catctccatc agggtcatgt gcacgacccg gcgctccatc
     9001 gccgacatcg ggtcgaagtg gtacgggcgg ccgctggtga gcaccttttg accgacgccc
     9061 tgcgcgagct ccttgaggct cttctcgcgc cgcgtgcgat agccgccgac gtcgagggta
     9121 atgcgcatgc ggtcgaagcc ctcggcctgc agcacgcgca gcgtcaggta ctgcagcgcg
     9181 tccagcgtgg cgccgtggcg cccgatgagc aggccgccgt cttcctcgct ggtattgagg
     9241 tcacagtgga gcccgtcttc ttcgatgtcg atgtcgacct cgacctcgac gccgaggagt
     9301 ccgagcacgg actcgaggaa ggagcgaacg acggggaccg ccttgccgta gttctggtcg
     9361 gcgttggcgg gggtttcggc cgggggagcc gcttgcttgg agtcagccat gatgttcttc
     9421 gattctccta ggacttggtg gcgacggcgg ccttcgtggg acggagttgg atgaccgtct
     9481 gtaccagcga cagcagcacg ttggcgaaga tgtagacaca aaggccggac ggcaggaaca
     9541 gcatcatgac cgtgaagatg atcggcatca tccacgccat catcttggcc tgctcgttgt
     9601 ccacgcccga gggctggatc ttcatctgga agtacatcag ggcgcccatc gcgagcggca
     9661 agatgtagta ggggtcctgc tgggtcaggt cggggagcca caggaagggc tcgcggtaca
     9721 gctcgaccgc cgtgttgagc atcgcgtaca gcgcgatcca gatcggcagc tggatgagca
     9781 tcggcaggca gccggcgagc gggttgacgc cggcgcgctt gaacagcgcc tgcatctcct
     9841 gggcctgacg cacgcggtca tccgcgtact tcttcttgag cgcctcgagc tccggctgga
     9901 cctcgcgcat cttcttcatc gagcgcatct gcttgaaggt cagcggcagc gtcacgacct
     9961 tgaccacgac ggtcaggagg atgatcgcca cgccccagat gccggtcagg ttgtagaaga
    10021 ggcgcagcag ccacagcatc ttctcgccga ggaagcgcgt caggccgccg aaccagcccc
    10081 agtcgaccgc gtcctcgagg tgcacgcccg ggacgacggc gaagctcgac agccgctcca
    10141 tctccttggt gccgaggtac aggccgaact cgtaggtccg cgactcgttg ggctcgagcg
    10201 tgacggtgtt cgagccgatc gtgctgacca gcatcgcgcc gtcgtcgctg agcttcacct
    10261 cgcacatttg cacggggtgg gtcggcacga ccgccgacac gaaatacttg ctgtcgagcg
    10321 cggcccagcg gatctggccg cggatcgtcc cggaggactc ggagacgtcg ctcgagtcga
    10381 actcctcgac gtcatcggct gtcgcgcaca ggccacggtg gatgtcgtag ctgctcgcct
    10441 ccttgggcag gccgtactgg ctcttgagca gcatgcggtg ggcttgccgc ttgctcgagc
    10501 ggttgagcac cttgacctgc agctgagcct cgtagccccc ggcgaccttg aggtcgccga
    10561 ggatctcgac gtcgaggtcc ttgtagcgca ggctccacgt cgtgtcggtc ttgccgacga
    10621 cctcccagcc ggcgttgcgc ggcatccgga agtcggtctc gttggcgtcg aaggtgaccg
    10681 ccagcgagtc ggcgccgccg aggtgcagga agtcgtcgcc gatgccgtcg gcgcgcacga
    10741 actgcttgct gagcagctcg gtgcggcgaa tgatgccgcc gacgttgccg acgtcgagcg
    10801 cgatcaggtc ggtcttgagg cggtgcgtct ccgcgggcgc ctcgttgtgc ggcttgctgt
    10861 ccggcgccgc ctcggcgtcg gccttggcgt cggcgccacg cggggcgtcg acctcggccg
    10921 catcagcagt ggaggtactc gcggtcgcgg ccttcggctc cggcatcggc ggcgcgaaga
    10981 aactcgcgta cacgatccag atcaacacgg agacgacgat cgccaacagg gtacggcgct
    11041 gaaaatccac gactcagcag ttctccagag aagagggggc gtccatcacg gacggagacg
    11101 agcgctgctt cgaggtcttg gcctcgggca gcgcgggcgg caggtcgagc cccccggggt
    11161 gccacgggtg acagcgcagc agccgacgaa gggtcagcca gccgccgcgc agggcaccgt
    11221 ggtcacggat acattcacaa gcgtagtgcg agcagctcgg cgtgaatcga cagcacggcc
    11281 cgagcagcgg ggacaggacc cgctgataca cccggatgct cgcgatcatg actctgctca
    11341 gcacgaagct cccagcggcg ggcgcttgcc cagcagctcc atcgcggcga cgacctcgtc
    11401 gtaaccgacg gacgctgcgc tctgctttgc gacccagacc atatcgtagc cgggtgtaaa
    11461 gctgccacgt ttgcgccgga atgcctcccg aacgtagcgt tttatgcgat ttcgcacgac
    11521 cgcgcacccg accttgcgcg tcacggtgat gccgagccgc accacatcgg agcgctcttc
    11581 gaccggacgc gaggctggtg gcaccacgaa cacgagaaag tggcgcaggt gatgcttggc
    11641 acctgcgcct tgaatggcga gaaactccct gcgcttcctt acgcggatcg cagcagggaa
    11701 gccttcaggc ttcatttgcc gccgatcgac acgctcagac gtttacggcc gcgacgacga
    11761 cgggcggcga ggaccttgcg gccgccgcgg gtcgccatgc gggcgcggta cccgtgggta
    11821 cgggcgcggc tcaggttatg gggctggtac gtgcgcttcg acatgggaat tccgtgagct
    11881 gtggggccgg gagagggatt gcggcgagac ctgcaacgct tgcatgcgat gccgtagaac
    11941 gcgggcccgg caaaggggcc ggatcgatac cagcgtgacc ccggtctgtc aacgcaaccg
    12001 cccacggcgg cgacgcccgc tgcatcctta tgtgtagcgg ctccgacggc gccgaccgcc
    12061 ccgagtgtcc ggcggtcact tgtctccagc ggtcactgga tccttccaca cgccggggag
    12121 acattctcct tggcacacct acacccaacc ctggaaacga ctttcacctg ggcgctcaaa
    12181 ctggcttcgc aagccgactt gcggcctcgg agcggcgcaa aaccgcttgg tctccacctg
    12241 cctgcgcaac ctgacccgaa gctcgcggaa atgtcctgtg atcacgcctg gatcgctcga
    12301 tcgcgcggcc ttgtcggggg tcgttcccga gcgagggcgg ggcctgtccc ccgaaaagcc
    12361 gttgtgatcc cggaggctta gaaattccct gtcgtcagcg tcttgtcccc gaagcaccag
    12421 cccgagtaga accctcctcc ccgcgccgcc cggcgcgcct tacaacttac gttccgcggc
    12481 gcacgcgcgc tctctgcctc cctgtcaggc ctccccgacc cccctcgggt gatctcacag
    12541 gatgtggata acgtccgcat tgcgccgctc aactcgttgc aattccgtct ttttccgagt
    12601 tctccacagc cctttcaagg ctgtggatgg agggttcatg agcgatattt ggagcgaggc
    12661 actgcaagga ctgcaaccgc ggctcgggtc ccagacgtat gacctgtggc tgcgcccgat
    12721 cgcgctccgg cgagtcaacg gcacccggct gcacctgacc gcgcccaacc gcttcatgaa
    12781 ggagtggttc gagaaccact atctcggggt cgtgctcgac gagctgcgcg ctcgcacatc
    12841 ccagacctat gatgtcgaga tcgaagtcac cgccgacgcg ccggccgctc cccccgccac
    12901 cgcaccggct gcggtcccca ccgagcctgt ccgccccgcg agtccgccgc cggtcccggc
    12961 cctggtcgcc gctccgccgc cgcagctgtc gggccgctat cgcttcccca actttattaa
    13021 aggcgccggc aacgagctcg ccgcctcggc cgcctgggcc gcggcgaacg agcccgggac
    13081 ccgcttcaat cctctcttca tctacggcgg cgtcggcctc ggcaagaccc acctgctgca
    13141 cgcgatcggg caccacatcc acgccacgcg tccggagcag cgcatcgtct atcagagcgc
    13201 cgagcgcttc atgaacgagt tcatcgccgc ggtgcgcttc aatcagttcg acgagtttcg
    13261 cagccgctac cgcgaccagt gcgacgtcct gctgatcgac gacatccagt tcatcgccgg
    13321 ccgcgaccgg acgatggacg agttcttcca cgtcttcaac gtgctctacg aggccgagaa
    13381 gcagatcgtc gtcaccgccg accgcatgcc ctcggagatg cagggcatgg aggaccgcct
    13441 gatctcccgg ctcaactggg gcctcgtcgc cgacatcaag ccgcccgacc tcgagacccg
    13501 cgtcgccatc ttgcgccaga aggccgagca cgaccacatc gtcatcgccg acgaggttac
    13561 gctccacctc gccaacatcg tgcgctccaa cgtgcgtgag ctcgagggtt cactggtccg
    13621 cgtgtcgacg ctcgcctccc tcaagaacgt cccgatcacc tgcgagttcg tcagcgagct
    13681 gctcggcgcg cccgcgggca gcgcgcagcc gcctcccgtc agcatcgagc aggtgcagaa
    13741 ggtggtctgc gcccacttct cggtccgcgt ctccgacctc aagggtccgc gccggcacca
    13801 gggcatctcc aggccccgca tgatcgccat gtatttgtgc cgtcagttga ccagcgccag
    13861 cttcccggag atcgggctgc gcttcggcgg caaggaccac tcgacggtca tcaacgcctg
    13921 caagcgcatc gagtcgatcc aggccgagga ccgcgagctg cgggccaccg tcgacaccct
    13981 gcggcgccag ctgaccggcg ccgagtccac cagcacgacc gtccacacgg gctccggcgg
    14041 cgccacgatc gcagcgcccg gcgccgtcct gtaatccttc cctcacgtcg cgccgtcttc
    14101 tgacgccggg ccgccttcac ggtgccccgg cgtttggctt gtcccgagaa cgggtcgtcc
    14161 acagccccct gtggagtcac ctgcgaacaa gccggcaaac gcccggctgg cgccccgccg
    14221 gggataactc ccccgatgat ccccggcgcc ccacacccga cgaatttcct gatctacgag
    14281 tcttctcggt gcgatacact ccgctccccc cgctccacag cgcagatcaa gaagacgaga
    14341 atttaagaat taaagcctta agatcttaac tcgttaagca gctgcctgtg ctccacactt
    14401 cctcaccgat ctgaccctcc tcaagagagt cgaaagatcc gagtcaaaga ggcctcgatg
    14461 gaattcgaga tcgaccagtc caagttcagc cacgcgctcg cccaggcaca gaccgtcgcc
    14521 gacaagcgca gcaacaacat gccgatcctg gcgaacgtgc ttctgcgggc gaccaaggac
    14581 aacaagctca tctgcagcgc gacggacatg atgatctcgg tgaccgagac catccccgtc
    14641 gaggtcaaga cgcccggggc gctgacgctc ggcgttcgct acctgcacaa cgtcgtccgc
    14701 accttgccgt ccaagccgct gcggatccgc gggctcgaga accactgggc gcagctcagc
    14761 gtcggccgca gcgagttcaa gctgatgggt caggcgccgg gcgacttccc ggagctgccc
    14821 gacaccaagg gcatcaagtt cacgcggctc ccgggccaca ccgtcgccga cctgatccaa
    14881 aagacccagt ttagcgtctc gaccgacgag gcgcgggtca acctcaacgg cgtgctgttc
    14941 gagagcagcg gcgagctcgg cacgatggtc tcgacggacg gccaccgcct caccaagctg
    15001 tcgctgccgc tgacgggccc caagctcgat cgcggcatca tcatcccgcg caagggcatg
    15061 ctggagatca agcgcgtgct cgaccgcgtc tccggcgagg tcggcctcgc ggtcgacggg
    15121 cagcacctgt acctgcaggc cgaggagctg ctgctgacgg tcaagctcaa caacgtcgtg
    15181 ttcccgccgt acgagcaggt catcccgcgc gagttccggc gccgggtcac cgccctgcgc
    15241 gacgagatga tcgacgtgct caagcgcgcc gaggtcatgg ccccggagaa gaccgcgacg
    15301 gtgcggctcg agctcgacaa gggcagcctc aagctgaccg ccgacaaccc cgacctcggc
    15361 gtcgcgcacc aggagatcga gatcgagtac tcgggtgagc cgctggtcgc cggcttcaac
    15421 gcgcgctacc tgatcgaggt gctcgaggcg atggacacca agcaggtgca cctcgacttc
    15481 cagggcgagc tcgacccctg cgtgcttcgt ccggtcgacg gccccgacta cctcggcgtc
    15541 gtcatgccga tgcggatctg atctctgcag ctcgagcacc tcagcatccg cgagtttcgc
    15601 aacctcggcg cgggcgaagt cagcttcggc ccgcgcttca ccgtgctgca cggccacaac
    15661 ggcgccggca agaccaacgt cctggaggcc ctgtacctcg tcggcaccct gcggtcgttc
    15721 cgcaccgccg acctcggccc gctcgtccgt cacgggcagc ccgaggcccg cgtcgaggtc
    15781 cgcgggctcg acccccgcgt cggcatcagc tcgaagctcg tcgtccagct cagccgcacg
    15841 gagcgggcca cccgccggct cgcccaggcc gacggcaaga ccgttcgctc gggcctcgat
    15901 ttttatggcc gccttcgcgc catcctgttc acgccggagg acctcggcgt gctgcgcggc
    15961 tcgccgaccg gccgccgcca gttcatcgac cgggtcctgt tcgcccgtga gcgcacacac
    16021 atttctgata cgcaggacta cgagaagctc ttgcgctcgc gtaaccgcgt gctgcgcgag
    16081 gaggagggca ctccgcgcca gcgcgacgac ctgctggcct cgtacgaggt cgggctcgcc
    16141 gaggtggggg gtcgcatctg gacgcgccgc gaacggatgc tgtcggacct gcacgcgcgc
    16201 ttcacagccg cgttcgcagg catccacggc gccaccctgc cagcgacgat gacttacgtg
    16261 agcaaagtcg gcgcgctggg cggccggacc gcgcaggacg tgctcggcga ggccctcaag
    16321 gagcgccggc gccacgacat cgtccgcggc acgaccaccg tcggcccgca ccgcgacgac
    16381 ttcgacatgc agctcgggga gcagtctgcc agcacctacg cctcgcaggg gcaggcccgc
    16441 gcgctgatcc tcgccttcaa gatcgccgag ctgcaggccg cgcgcgagct gaccggcgag
    16501 gccccgctgc tgctgctcga cgacgtgtcg agcgagctcg acccggagcg ctcggcgcag
    16561 ctgttcgcgg ccctggtgcg ggaggccggc cagtgcgttt tgacgaccac cgcgccgcac
    16621 tttatcggcc tcgaggcggg tgaagattgc cggtacatcg aggtcaaggg cggccggatc
    16681 gagccggtcc ccaccccacc ctgagccggc cccccgatct acccgtgcgg gcgcgaattc
    16741 cggcgaattg gcgccgtctg cgagcttgga agcacggccc ggtcgtgcta ccttccgcac
    16801 gctgtttacg ccgcgaattc acagagaatt tcactgtcga tttcgccatc gcggcagccc
    16861 tgagttccat gtctgaagcc gacaagacgc aagccgctga cgagtacaac gccgactcca
    16921 tttcggtgct cgagggtctc gaggccgtgc gcaagcggcc aggcatgtac atcggcgaca
    16981 ccagcgatgg ttccggcctg cacaagatgg tcttcgaggt cgtcgacaac tcgatcgacg
    17041 aggccctcgg cggccactgt gacacgatcg aggtcatcat ccaccccgac aactcggtct
    17101 cggtggagga caacggtcgc ggaatcccga ccggcccgat ggaggcgagc ggcaagacca
    17161 tggacgccgc gatcgtcatc ctcacggtcc tgcacgcggg cggcaaattc aataacaact
    17221 cgtacaaggt ctcgggcggt ctgcacggcg tcggcgtctc ggtcgtcaac gcgctgagcg
    17281 actggctgca gctcgacatc tggcgcgagg gcaaggccca cgcggtccgc ttcgagcgtg
    17341 gcaacccggt gaccgaggtc gtgcacgtcg gcccggccga ccgccgcggc acccgcgtcc
    17401 acttccacgc cgaccccctg gtcttcacga tcaccgagat cgacttcgag gtcctcgcca
    17461 accgcttccg cgagctcagc tacctcaaca gcaacgtcaa gatgacgctg cgggacctgc
    17521 ggaccggcaa ggagcgcgtg ttcgacggcg agggcggcgt gccgagcttc gtcaagctgc
    17581 tcgcgcagaa caaggaggcg atcggcgaca tcgtcgtctt caataaagac atcgagttcg
    17641 agcacgaggg ccgcaccgcg gtgctcggcg tgcagatcgc aatgcagtgg acggacgtct
    17701 atcaggagac gatcctctgc tacaccaaca acatcgccaa ccgcgacggc ggcacccacc
    17761 tgaccgggtt tcgcaccgcg ctgaccaagg tgatcaacaa gtacgccagc gagagcggcg
    17821 cgctcaaggg tcacaaggac acgctcgctg gcgaggaggt ccgcgagggc atcgtcggcg
    17881 tcgtctccat tcgccatcca gatccgaagt tcagctcgca gatcaaggac aagctggtct
    17941 cgggcgaggt caccagcctg gtctcgaccg tcgtcaccga ggagctcagc cgctacctcg
    18001 aagagaaccc gcgatctgcc aggttgatcg tcgagaaggc gctgctggcc gcgagggctc
    18061 gcgccgccgc ccgcaaggcc cgcgagacga tcacccgcaa gggcatcctc gacggcctct
    18121 cgctgcccgg caagctcgcc gactgccagg agcgcaaccc cgagcacgcc gagctgttcc
    18181 tcgtcgaggg tgactccgcc ggcggctccg ccaagcaggg tcgcgaccgc aagacccagg
    18241 cgatcttgcc gctacgcggc aagatcttga acgtcgagaa ggcgcgcatc gacaagatgc
    18301 tctcgagcca ggagatcatc acgctgatca ccgcgctcgg caccggcatc ggcctcgaca
    18361 actacgacat cggcaagctg cgctaccaca ccatcgtcat catgacggac gccgacgtcg
    18421 acggctcgca catccgcacg ctgctgctga ccctcttcta tcgccacttc cccgacatca
    18481 tcgagcgcgg ccacctgtat atcgcccagc cgccgctcta caaggtcaag tccggcaagg
    18541 ccgagtcgta cctcaagaac gacgccgccc tcgaggactt catcatcagc aacgcggtcg
    18601 agagcatgcg cctgtcgatc ggcgggcact tcgtgatgcc cgaggtcctc ggcgagatcg
    18661 cccgccaggg cctgcggtac cgccacctga tggagtcgat ggcccgcgag caccgggcac
    18721 cggtcgtcga ccacatgctc aacgacctgc atcagggcgc gatcaagccg gacgtgttct
    18781 cggacgagcc cgcgctcgcg gacttcggct cgcgcatgca ggcggcggtc gctcagcaga
    18841 tgccgctcgc gcgggtcagc ttcgaggtcg tcgacgagtc cgtcgacccg gcccgcgagg
    18901 ctgcccgcgc gaccgccggt atttcgatcg agcgccgcgc gatccgcctg cagatcatcg
    18961 ccgacggcgt cgcccagtac gaggtcgtcg accacgagct gctcaactcg taccccgtgc
    19021 aggagatgct caagatccgg cactacatgg acaccctcgg cctgccgccc taccgcctct
    19081 accgcggcga gaacgtcgtc gccgagccgg cccgcctggt cgacgtcgtc cagcacgtcg
    19141 gcgagctcgg ccgcagcggc ctgcagatcc agcgctacaa gggcctcggc gagatgaacc
    19201 ccgagcagct gtgggagacg acgatggaca tcacccgccg cgccctgctc aaggtccgcg
    19261 tcggcgacaa cgtcgacgcc gacaacgtgt tctccgtgct gatgggcgac aacgtcgagc
    19321 cgcgccgcga cttcatcgtc aacaacgccc tcaacgcccg caacctcgac atctgaggcc
    19381 cgggctcaac gcctcgagct cgcgtggcct cgacctgtcc caaagaccag ccgatttcca
    19441 ggccgacgca caccgcgtca gcccggcgcc tggtcccacg ggaccttgtc gccgcagtgc
    19501 agcatcatca tcccgtgata gggattgacg atctcgccgc tgcgctgcac ccagtgggcg
    19561 cccttattat tgaaggccat cgggcag
//
LOCUS       DASWNJ010000004         5700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_494572, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000004 DASWNJ010000000
VERSION     DASWNJ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494572"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1938
                     /locus_tag="VGB85_00100"
     CDS             <1..1938
                     /locus_tag="VGB85_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132433.1"
                     /translation="VRAEDLGFALAGFANVPGQNYYVAKLSTVPLAFNSVLEAGSDAD
                     GAGKPRNWLAIMAEDATTLYPEGGQEIRVGLGLGPYFEGQTCLPPVEHAMSGSSAVDS
                     VGFIALRGSFRGSAEDEVTTMAAVARVGFVDDASSGGHGPTPDADSYLKLHIAEFIDG
                     RFFGIDRKFDGPWSALDLEWCPINFVGYNLGNGDRADLILLRTLLSTGSSSWSGYDGQ
                     GAVFTPGLNDPGVGAPQGGDVEIADLGLAIPRSLIDAPSFAATANLLPEGVNSPLNRC
                     KFAFIGAFDSQDLIWRLLEQRGWGMSFVGGRFGLYSRPQLLDIEDAVLTLGPDDFAVD
                     DVQFVERADLAPTIARDGFEVTYGEPLVEGTGSDLELKVTVRASDPASRTRRSNNVES
                     IEGMGLIATRLWDSGQGPQPWMGAWSKLAGDLSNWYAQPWVAVDVPIMWSVAQYLGPG
                     AVILFSSLFAPTREGVYGNSNKLARVISMTLNLNELTAEVRVLVQPGDATKRRWAPVA
                     MVLDDVSTVEQRHDAAHETMFCYADAFGHGEGTHDLAWFVEPAWSSIGGEARVQGWQH
                     DGRRWEATFSLTVVSVDTTADSLTYSKLEGTFYEARPTILVLAPWAEQSPGSWPCALF
                     SVVTRSDTKFGGVPTQGYPLL"
     gene            1935..3215
                     /locus_tag="VGB85_00105"
     CDS             1935..3215
                     /locus_tag="VGB85_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132434.1"
                     /translation="MTLSPISKGDLAFLCLPAGAGAAAAVVDVSEQRTSITNNTSYVS
                     ETASARTAIAAPRTICVVVDMTSTSSGRLIQHGSGASAYGYAINKTGTSIACAENNLG
                     RITVTVPGLTAVARKVLVHWAQRVEGASVVSECAVYNFFTLEWNFGSAVHAAYAPLAT
                     DKLTIGASSTGSSAYSLSMTAFHAVSIGRRFHSTTETSLDFVSTPTPPTMLGRRRTPL
                     PTGVAASLAIVGDGQFAGPQYLMALAATRESDSRLVTPLVNIVVANPTSEVLAPTARY
                     KRAAPGAPTYRLSTRWLWCAVPGPKVNAARCRIHVAVTGTGGVCPIYFRLYSLANLPM
                     GQVNPPQLVSYFSPTVSIAASTGAAGVVLDLGMVRLARDDAGLTALALALDFNHGGGD
                     AFATNVKIRAVSIEPYSADLSGGGFGDVDDKEGY"
     gene            3218..4534
                     /locus_tag="VGB85_00110"
     CDS             3218..4534
                     /locus_tag="VGB85_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132435.1"
                     /translation="MGIKRVNISNTSSGTAPLGPLRAGQYAKRSGVMSAMADADRALG
                     NGRRVLARVGLHGEAAHQVVSTNTTSATQTYPTKTASRVALRWKGPLTPGHFPTVSAI
                     VVPAGATQKAAAGGFVLDAVVGWIDIIAEFSGPASETVTHTFALPASGNQYGGEGADA
                     GWAWAALRKVGPAALAPAGALQNAGTMRTWSEGVTVELTVIYRGGVRCVDLVLQEQPF
                     AYARSVGIDAASTFSSPLAVSGDGKPAATYPSSFPLEELSPTDPTFGTALLADVVHRQ
                     HSGWGPVLAQWSGWRESQAVTATAAVGVSTTSLTYLNMLDTSLTTWSSASPGWSLASG
                     GQAQQWLSSNSHRTTRDVDACVPVRCWVYGSRTGSGASTIRFQSENFSIVEVSITSST
                     DGWWSATGHLRCGLGPEDTSTLQVLGKLAAAGTLTIRHIVVEYVNA"
     gene            complement(4616..5281)
                     /locus_tag="VGB85_00115"
     CDS             complement(4616..5281)
                     /locus_tag="VGB85_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132436.1"
                     /translation="MTAVHYSPMQPNASGRALAACGVWSPSFTADPDLITCPACLSLR
                     KPTPTPTKTGTNNMRTLLTLTTALLLAAACDPEDPASTTNQSEPITTSEDASSSGGGE
                     TDSGETGGGSESTGGSTGGDSTAGDEPTSSTGTGGSGEGDGSGSTDADAATCFASCSD
                     VDCAPGLICAPHPDTMELTCVMPCSSISGCDEVVPFLCDGDAMMAAECRLEFAVPLCF
                     PVE"
     gene            complement(5278..5499)
                     /locus_tag="VGB85_00120"
     CDS             complement(5278..5499)
                     /locus_tag="VGB85_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132437.1"
                     /translation="MSRRVHMPKLGHETSILEKAPLLCGQTRDTGTLVTVFAYLATCS
                     RCVKANAVITYQHVQAKLRRERGEEQVSP"
     gene            complement(5496..>5700)
                     /locus_tag="VGB85_00125"
     CDS             complement(5496..>5700)
                     /locus_tag="VGB85_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132438.1"
                     /translation="KRARETGGRQDPRSRLLRARATTLTARLAELRKRLTAAKELSMS
                     IEAMVIESNADDAVEETAAARAA"
BASE COUNT          924 a         1910 c         1900 g          966 t
ORIGIN      
        1 gtccgtgcag aggacctggg cttcgcgctc gcggggtttg ccaacgtacc cggccagaac
       61 tactacgtcg ccaaactctc gaccgtaccc ctcgccttca acagcgtact cgaagccggt
      121 agcgatgcgg acggcgcagg caagccgcgc aactggctcg ccatcatggc tgaggatgcg
      181 acgacgctct acccggaggg tgggcaggaa atcagggtcg gcctcggact cgggccgtac
      241 ttcgagggcc agacctgtct gccgcccgtc gagcacgcga tgtcgggaag tagcgctgtc
      301 gacagcgttg gctttatcgc gctccgtggc agcttcaggg ggagcgccga ggacgaggtg
      361 acgaccatgg ccgcggtcgc cagggtcggc ttcgttgacg acgcaagcag cggcggtcac
      421 ggtccgacac cggacgcgga cagttacctc aagctccaca tcgccgagtt catcgatggc
      481 aggttcttcg gcatcgatcg caagttcgac ggtccatggt ccgcattgga cctcgagtgg
      541 tgccccatca acttcgtcgg gtacaacctc ggcaacggcg accgcgccga cctaattcta
      601 ctgcggaccc tcctgtcgac cggtagtagc tcctggtctg gctatgacgg acagggcgcg
      661 gtgttcacac cgggtctcaa tgaccccggc gtaggcgctc cgcaaggcgg cgatgtggag
      721 atcgccgacc tgggcttggc catcccgcgg tcgctcatcg atgccccgtc gttcgccgca
      781 acagcgaacc tcctcccgga gggcgtcaac agtccgctga accgctgcaa gttcgcattc
      841 atcggagcct tcgacagcca ggacctcatc tggcgtctac tcgaacaacg tggttggggc
      901 atgagcttcg tggggggccg cttcggtctc tactcccgtc cgcagcttct cgacatcgag
      961 gacgctgtcc ttaccctcgg gccggacgac ttcgccgtcg acgatgtcca gtttgtggag
     1021 cgcgccgact tggcccccac gatcgcacgc gacggcttcg aggtcacgta cggcgagccc
     1081 ttggtggagg ggaccggcag tgacctcgag ttgaaggtga ccgtccgggc ctctgacccg
     1141 gcgagtcgca cccgccgctc caacaacgtc gagtcgatcg aaggtatggg tctcatcgca
     1201 actaggctct gggactccgg tcaggggccg cagccatgga tgggggcatg gtccaagctc
     1261 gcaggcgatc tgtcgaactg gtacgcgcag ccctgggtgg cggtcgacgt gccgatcatg
     1321 tggtcggtcg cacagtacct tggccccggc gccgtcatct tgttctcctc gctcttcgcg
     1381 cctacgcgcg agggcgttta tggaaacagc aacaagctcg cccgcgtcat cagcatgacg
     1441 ctcaacctca acgagctgac cgccgaagtg cgcgtgctcg tgcaacccgg tgacgctacg
     1501 aagcggcgct gggcccctgt cgcgatggtg ctggacgatg tatcgaccgt cgagcagcga
     1561 cacgacgccg cacacgagac catgttctgc tacgccgacg cgttcgggca cggcgaaggc
     1621 acccacgatc tggcctggtt cgtcgagccg gcgtggtcct cgatcggcgg cgaagcacgg
     1681 gtgcaaggct ggcagcatga tggtcggcgc tgggaagcta ccttctcact caccgtggtg
     1741 agcgtcgaca ccacggccga ctcgctcacc tacagcaagc tcgagggcac gttctacgag
     1801 gctcggccga cgatcctcgt gctcgcgccg tgggctgagc agtcgcccgg aagctggccg
     1861 tgcgccctgt tcagcgttgt gacgcgcagc gacaccaagt tcggcggcgt gcctacccaa
     1921 ggataccccc tcctatgact ctctctccga tctccaaggg agacctcgcc ttcttgtgcc
     1981 tgcctgccgg cgcaggtgcg gccgctgctg ttgtcgacgt gtccgagcag cgcacgagca
     2041 tcacgaacaa cacgtcgtac gtgagcgaga cagcctccgc ccgtacggcg atcgccgcac
     2101 cgcgaacgat ctgcgtcgtc gtcgacatga cgagcacgag cagtggccgg ctgattcagc
     2161 acggctcggg cgccagcgcg tacggctacg cgatcaacaa gacaggcacc agcatcgcct
     2221 gcgcagagaa caacctcgga cgcatcacag tcacggttcc aggtctgacg gcggtagcgc
     2281 gcaaggtgtt ggtccactgg gcccaacgag tcgagggcgc gagcgtcgta agcgagtgcg
     2341 cggtctacaa tttttttacc ctcgaatgga acttcggctc cgccgtgcac gccgcctatg
     2401 cgccgctcgc aacggataag ctgaccatcg gggcttcgtc gaccggaagc tcggcctact
     2461 cgctcagcat gacggccttc catgcggtct cgatcgggcg tcgctttcat tcgaccactg
     2521 agacctcgct tgacttcgtc agcacaccaa cgccgccgac gatgctgggg cgccgccgga
     2581 ccccactccc gacaggtgtc gctgcgagcc tggccattgt cggggacgga cagttcgccg
     2641 gtccgcagta cctcatggcc ctcgctgcga cgagggagag tgactcgcgg ctcgtcacgc
     2701 cgctcgtcaa catcgtcgtc gctaacccga cgagcgaggt cctcgcccca acagcccgct
     2761 acaagcgggc ggccccaggc gcgccgacct accgactctc gaccaggtgg ctgtggtgtg
     2821 cagtgcccgg cccgaaagtg aacgccgcac ggtgccgcat ccacgttgcg gtgacgggca
     2881 cgggcggggt ctgccccatc tacttccggc tctactccct ggcgaacctc cccatgggcc
     2941 aggtcaaccc gccgcagctc gtgtcgtact tctcgcctac ggtctcgatc gcggccagca
     3001 ccggagcagc gggtgtggtc ctcgacctcg gcatggtccg acttgcacgc gacgacgccg
     3061 gcctgaccgc gttggcgctg gcgctggact tcaatcacgg cggcggggat gccttcgcta
     3121 cgaacgtaaa gatcagagcg gtcagcattg agccctacag cgccgatctt tccggcggtg
     3181 gcttcggcga cgtggacgac aaggaaggct actaggcatg ggcatcaagc gcgtcaacat
     3241 cagtaacacc tcgagtggca ccgccccgct cggcccccta cgagcagggc agtatgccaa
     3301 gcggtccggt gtcatgtccg cgatggctga tgcggaccgg gccctgggca acggccgccg
     3361 cgtgctggcg cgtgtcggcc tgcacggcga ggcagcacat caggtcgtgt cgacgaacac
     3421 aacgagcgcg acccagacct accccaccaa gacggcgtcc cgcgtggcgc tgcggtggaa
     3481 gggcccgttg acgcctggtc actttccaac ggtgtccgcg atcgtcgtgc ccgcaggtgc
     3541 cacccagaag gccgccgctg gcggcttcgt gctcgacgcg gtcgttgggt ggatcgacat
     3601 catcgccgag ttcagcgggc ccgcttccga gaccgtcacc cacacgttcg ctctaccggc
     3661 gtcgggtaac cagtacggtg gcgagggggc tgacgcaggt tgggcatggg ccgcgctacg
     3721 caaggtcggg cccgctgctc ttgcaccggc cggggcgctg caaaacgccg ggaccatgcg
     3781 cacctggtcc gagggcgtga ccgtcgagct gaccgtgatc taccgtggag gcgtacgttg
     3841 cgtggacttg gtgctgcaag aacagccatt cgcgtacgcg cgcagtgttg gcatcgacgc
     3901 ggcctcgaca ttctcgagcc cgcttgcggt gagcggcgac ggcaagcccg cggcgacgta
     3961 cccgtcgagc ttccccctgg aggagctgag cccgaccgac ccgacgttcg gcacagccct
     4021 cttggcggat gtcgtgcacc gccagcacag cggctggggc cccgtgctgg cgcagtggtc
     4081 gggctggcgg gagagccagg ccgtgaccgc aacggcggcc gtgggtgtca gcacgacctc
     4141 gctgacctac ttgaatatgc tcgacacgag cctgaccacg tggtcctcgg catcgcccgg
     4201 ctggagtctc gccagcggag gtcaggccca gcagtggctg tcctcgaact cgcaccggac
     4261 gacgcgcgac gttgatgcct gcgttcctgt tcgctgctgg gtgtacggct cgcgcaccgg
     4321 cagcggcgcg agcacgatcc gctttcagag cgagaacttc agcatcgtcg aggtctcgat
     4381 caccagctcg accgatggct ggtggagtgc aacggggcac ctgcgctgcg gactcgggcc
     4441 cgaggacacg agcacccttc aagtccttgg gaaactcgcg gccgccggca cgctcaccat
     4501 ccggcacatc gttgtggaat acgtcaacgc ctgagccaag ggaccacgtt gggccgagaa
     4561 cggagaaggg cccggtagca gtgctaccgg gcccctctcg ctccaaaccg aacacttact
     4621 cgacgggaaa gcacagcggt acggcgaact cgagccggca ctcggcggcc atcatcgcgt
     4681 cgccgtcgca caggaacggg acgacctcgt cgcacccgga tatggaggag cacggcatca
     4741 cgcaggtcag ctccatcgtg tcggggtgcg gcgcgcagat cagccccggc gcgcagtcga
     4801 catccgagca cgacgcgaag cacgtggcgg catccgcgtc cgtggagcca ctgccatcgc
     4861 cctcaccgct gccccccgtc ccggtggagc tggtcggctc gtcgcccgcg gtgctgtcac
     4921 cgccggtaga gccgccggtg ctctcgctgc cgccgccggt ctcgccgctg tcggtctcgc
     4981 cgccgccaga gctcgaggca tcctcgctgg tggtgatcgg ttcgctctgg ttcgtggtgg
     5041 aggcggggtc ctcggggtcg caggcagcgg caaggagaag ggcggtggtc agggtcagca
     5101 gggtgcgcat gttgttggtt ccagtcttgg tgggcgtggg tgtgggcttg cgaagcgaga
     5161 ggcaggccgg acaggtgatg aggtcggggt cggcggtgaa cgacgggctc cagaccccgc
     5221 acgccgcgag ggcgcgacct gatgcgttcg gctgcatcgg cgagtagtgg acggcggtca
     5281 tggcgacacc tgctcttccc cgcgttcgcg tcgaagcttc gcctgcacgt gctggtacgt
     5341 gatgaccgcg ttggccttca cgcagcgcga gcacgtggcg aggtaggcga agaccgtgac
     5401 gagcgtgccc gtgtcgcggg tctgtccgca gagcaacggc gccttctcga ggatgctggt
     5461 ctcgtgcccc agcttgggca tgtggacgcg gcggctcatg cggccctcgc agccgcggtc
     5521 tcttcgaccg catcgtctgc gttgctttcg atcaccatgg cctcgatgct catcgacagc
     5581 tccttcgctg cggtgagacg cttgcgcagc tcggcgagcc gcgctgtgag ggtcgttgct
     5641 cgggctcgaa ggagccggga gcgagggtcc tgacgcccgc cagtctcacg agcacgcttg
//
LOCUS       DASWNJ010000005         3799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_70852, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000005 DASWNJ010000000
VERSION     DASWNJ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70852"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VGB85_00130"
     CDS             <1..810
                     /locus_tag="VGB85_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEY0132439.1"
                     /translation="HTLSMLSLKGPPRRLYAFRFGWEPVPESVSLRGGSKDVYLFEPV
                     TGAAIVYDDGWLLIDTGLNPDLIRDPKLRAAHYTTAAVGCVPEGDPLLRQIAAAGLSL
                     ADLKGCLLSHLHCDHGGGLRHLVDGPPIVIQAREHAFAMEEATLEHAYFKSDYDRPGL
                     AWRLIEGEVELAPGLRTVPTFGHTPGHQSFAVDLAVSGTIVLACDAADLRRNIDELIP
                     CGELTHPELASAAERSIERLHALDHQPNTEVWPGHDPDFWATRRGPPAGYA"
     gene            complement(836..2494)
                     /locus_tag="VGB85_00135"
     CDS             complement(836..2494)
                     /locus_tag="VGB85_00135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0132440.1"
                     /translation="MSAEVAGLLERLISQFAGPYDFLRELVQNSLDAGSDRAEVVLDT
                     HADVGAEADEVVFELRVSDAGSGMDEAIIDGGLTRLFASTKADDRTMAGGYGVGFVSV
                     FAWEPEAVLVQTGRGGEAWELVFYPDRRFEKTALNEPVEGTTVTLLRRGRAAERPAIA
                     EAVRDSLWRWCRFCKLELSFEDVASGEPPELIQDSPAPADSPLTIEEVQGDATIHVAF
                     AVPPSAVLLRRGLILAEGAPAELLAPFLPGLGRTAEHLQVWIDSPQLRTTIARDKVVD
                     DAGQRATLARAAAAIATLRGRLLAAIEAVVAEPSPWTAERHAHYAHLHAHLGRELAHV
                     GAAARERPLLRDLAGGRALTVSALATLLRGRPVVTATPAELDSPVLAAAGRLGFPVLA
                     AEPADLAWLKELLTGMGPIVPISQALWFERYNDEAASLRAAVDRTVRAAGMLVHVQLG
                     GGSEAGDRPGLVGLEVGTDAAGFLVVYGPGSWPETAWRAATVWIEDGLLQRAAQKAFV
                     AAPRTAAVTLACAVAAALGHEVEANERLSEALGRITTEMAPDAG"
     gene            complement(2537..3043)
                     /locus_tag="VGB85_00140"
     CDS             complement(2537..3043)
                     /locus_tag="VGB85_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132441.1"
                     /translation="MASLLATGCKGGSPSTEVAVHDRLWLTAVPKSMKAEVGAFIIIR
                     ASENKQYGFLFKGSLLRGYYDTFHWAPEGEGRATMRTIQDDKNHKIRMEPCEADTGFQ
                     YCVQLHGDPQGVVRYQSRKRWGLGRPKLKVLGFDLATELRDLAAEDGELAAALAEVEA
                     EALVDETR"
     gene            3197..>3799
                     /locus_tag="VGB85_00145"
     CDS             3197..>3799
                     /locus_tag="VGB85_00145"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HEY0132442.1"
                     /translation="MVSRARSLTLPLALACLPLLACGGEKPESQGVDPAKAAEMAKNA
                     VPAKKKPSRPVPSAAQVLDAAKKYFAPLPARAESASNPTTPEKIALGRQLYYDPRLSK
                     GQEVSCNTCHLLDKFGVDNELTSKGHKGQRGERNSPTVYNAAVHISQFWDGRAATVED
                     QAQMPITNPVEMAMADPAYVLRTIKSVPGYVEAFKTAFPGD"
BASE COUNT          553 a         1525 c         1203 g          518 t
ORIGIN      
        1 cataccttga gcatgctctc gctcaagggt ccgccgcgcc gcctgtacgc gtttcgcttc
       61 ggctgggagc cggtgcccga gtccgtctcg ctgcgtggcg gctccaagga cgtctacctg
      121 ttcgagccgg tgaccggcgc cgcgatcgtc tacgacgacg gctggctcct gatcgacacc
      181 ggcctcaacc ccgacctgat ccgcgacccg aagctccgcg ccgcgcacta cacgacggcc
      241 gcggtcggct gcgtccccga gggcgacccg ctcctgcgcc agatcgcagc cgccggcctg
      301 tcgctggcgg atctcaaggg ctgcctgctc tcccacttgc actgcgatca cggcggcggc
      361 ctgcgccacc tcgtcgacgg cccgccgatc gtcatccagg cccgcgagca cgccttcgcc
      421 atggaggagg ccacgctcga gcacgcctat ttcaaaagtg actacgaccg ccccgggctc
      481 gcctggcgct tgatcgaagg cgaggtcgag ctcgcgcccg gcctgcgcac cgtcccgacc
      541 ttcggccaca cccccggcca tcaatccttc gccgtcgacc tcgccgtctc cggcacgatc
      601 gtgctcgcct gcgacgccgc cgacctgcgc cgcaacatcg acgagctgat cccctgcggc
      661 gagctcaccc accccgagct ggccagcgcc gccgagcgct cgatcgagcg cctccacgcc
      721 ctcgaccacc agcccaacac cgaggtctgg cccggccacg accccgactt ctgggcgacc
      781 cgccgaggcc cgcccgccgg ctacgcataa tcatcgccgc gccccgcgac gacgatcagc
      841 cggcgtccgg cgccatctcc gtggtgatgc gcccgagcgc ttcgctcagc cgctcgttcg
      901 cctcgacctc gtgtccaagc gccgccgcga ccgcacacgc gagggtcacc gccgcggtcc
      961 tcggcgccgc gacgaaggcc ttctgggccg cgcgctgcag cagtccgtct tcgatccaca
     1021 cggtcgccgc gcgccaggcc gtctcgggcc acgaccccgg tccgtagacg accaggaacc
     1081 cagccgcgtc ggtcccgacc tcgaggccga cgagcccggg tcgatcgccc gcctccgacc
     1141 caccgccgag ctgcacatgc acgagcatgc cggccgcccg caccgtccgg tcgaccgccg
     1201 cgcgcagcga cgccgcctcg tcgttgtatc gctcgaacca cagcgcctgc gagatcggca
     1261 cgatcggccc cataccggtc agcagctcct tgagccacgc cagatcggcc ggctccgccg
     1321 cgagcaccgg gaagccgagc cgccccgccg ccgccagcac cggcgagtcg agctcggccg
     1381 gcgtcgccgt cacgaccgga cgcccgcgca gcagcgtcgc cagggcgctc accgtcagcg
     1441 cccgcccgcc cgcgaggtca cgcagcagcg gccgctcgcg cgccgcggcg ccgacgtggg
     1501 ccagctcgcg accgaggtgc gcgtgcaagt gcgcgtaatg ggcgtgccgc tccgccgtcc
     1561 acggcgaggg ctcggcgacg accgcttcga tcgccgccag cagtcgcccg cgcagcgtgg
     1621 cgatcgcagc ggccgcccgc gcgagcgtgg ctcgctgtcc ggcgtcgtcg accaccttgt
     1681 cgcgcgcgat cgtcgtgcgc agctgcggcg agtcgatcca cacctgtaag tgctccgccg
     1741 tgcgtccgag ccccggcaag aacggcgcga gcagctcggc cggcgcgccc tcggcgagga
     1801 tcagcccgcg ccgcagcagc accgccgacg gcggcaccgc gaacgcgacg tggatcgtcg
     1861 cgtcgccctg cacctcttcg atcgtcagcg gcgagtcggc cggcgccggg ctgtcctgga
     1921 tcagctccgg cggctccccg ctggcgacat cctcgaacga cagctcgagc ttgcagaacc
     1981 gacaccagcg ccacaggctg tcgcgcaccg cctcggcgat cgccgggcgc tcggccgcgc
     2041 gaccgcgacg cagcagcgtc accgtcgtgc cctcgaccgg ctcgttcaac gccgtcttct
     2101 cgaagcgccg gtcgggataa aacaccagct cccacgcctc gcccccgcgc ccggtctgga
     2161 cgagcaccgc ctcgggctcc cacgcgaaca cgctgacgaa gccgaccccg tagccgccgg
     2221 ccatcgtgcg gtcgtcggcc ttcgtgctgg cgaacaggcg tgtcagcccg ccgtcgatga
     2281 tcgcctcgtc catcccgctg cccgcgtcgc tgacgcgcag ctcgaacacc acctcgtcgg
     2341 cctcggcgcc gacgtccgcg tgggtatcga gcaccacctc ggcgcggtca ctgccggcgt
     2401 cgagcgagtt ctggaccagc tcgcgcagga agtcgtacgg ccccgcgaac tgcgagatca
     2461 ggcgctcgag cagcccggcg acctcggcgc tcacgcggcc ctaccccgca gtccgtttcg
     2521 gacccgtgcc aaagttttag cgcgtctcgt cgaccagcgc ctcggcctcg acctcggcga
     2581 gcgccgcggc cagctcgccg tcctctgcgg ccaggtcgcg cagctcggtc gccaggtcga
     2641 agcccaggac cttgagcttg ggccggccga gcccccagcg cttgcgcgac tggtagcgca
     2701 cgaccccctg cgggtcgccg tgcagctgca cgcagtactg gaagccggta tcggcctcac
     2761 agggctccat gcggatcttg tggttcttgt cgtcctggat cgtccgcatc gtcgcccggc
     2821 cctcgccctc gggcgcccag tggaaggtgt cgtagtaccc gcgcagcagc gagcccttga
     2881 acaggaagcc gtactgcttg ttctccgacg cgcggatgat gatgaacgcg ccgacctcgg
     2941 ccttcatcga cttcgggacc gcggtcagcc acaggcgatc gtggaccgcg acctccgtgc
     3001 tcgggctacc ccccttgcag ccggtggcca ggagcgaggc cacggcgacc gtgagcagac
     3061 aggaagtcgc atgactggaa aggcgagcgg ggtggttcac ggtgaggtat cgtcgcaagc
     3121 gtatcgcggt cgcgcaagcg cccatcgacc cccccacgcc acagacgccg ccatgacccc
     3181 ccgtaagccc gtttccttgg tttcgcgcgc ccggtccctc accctcccgc tcgcccttgc
     3241 gtgcctgccg ctgctcgcct gcggcggcga aaagccggag tcccaggggg tcgaccccgc
     3301 caaggccgcc gagatggcga agaatgccgt cccggcgaag aagaagccga gccgcccggt
     3361 gccctcggcc gcgcaggtgc tcgacgccgc caagaagtac ttcgccccgc tgcccgctcg
     3421 cgccgagtcg gcctccaacc cgacgactcc agagaagatc gccctcggcc gccagctcta
     3481 ctacgacccg cgcctgtcga agggccagga ggtgtcgtgt aatacctgcc acttgctcga
     3541 caagttcggc gtcgacaacg agctgacctc caagggtcac aagggtcagc gaggcgagcg
     3601 caacagcccg accgtctaca acgccgccgt gcacatctcg cagttctggg acggccgcgc
     3661 cgcgaccgtc gaggaccagg cgcagatgcc gatcaccaac ccggtcgaga tggcgatggc
     3721 cgacccggcc tacgtacttc gcacgatcaa gtcggtcccg ggctacgtcg aggccttcaa
     3781 gaccgcgttc cccggcgac
//
LOCUS       DASWNJ010000006         3413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_247376, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000006 DASWNJ010000000
VERSION     DASWNJ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247376"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VGB85_00150"
     CDS             <1..328
                     /locus_tag="VGB85_00150"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY0132443.1"
                     /translation="GALVHGDLLGQNLRIDFDGEPGVIDWEYAMLGDPAHDLAIVARG
                     ARRPFQSADGRARLLEAYNTRASFEVTKDDLRFFELALHVRWFTGCPDRKEDSRPILA
                     RLLATG"
     gene            complement(338..850)
                     /locus_tag="VGB85_00155"
     CDS             complement(338..850)
                     /locus_tag="VGB85_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132444.1"
                     /translation="MRTSHPLTAFSFYLSGRNQVLLSVADEVLDNLDRCAPGEEALPA
                     GPRARASDLMWLWTLGAYEVTRTMCQAHACFAARFLAEVGQLKVDLERVRVPNTKMEK
                     IKYDRKGATIPVRSDRSPDLWDAARKDLLVGDPADAVSARSLLAEYARVIASLRADEV
                     LMRHEDAFTG"
     gene            complement(854..1282)
                     /locus_tag="VGB85_00160"
     CDS             complement(854..1282)
                     /locus_tag="VGB85_00160"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEY0132445.1"
                     /translation="MQPSLELLRPVVALVVWTFVMWGWMYATRIPAIHRSGMRLDPTA
                     PRGEQMASLPAQVRWKADNYNHLFEQPTLFYALAIVLALVSRGVDTDAMLAWTYVGSR
                     VVHSLVQATFNKIELRFALFFVSSLVLAAMTVRAALAVVG"
     gene            complement(1334..1837)
                     /locus_tag="VGB85_00165"
     CDS             complement(1334..1837)
                     /locus_tag="VGB85_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132446.1"
                     /translation="MRHAWLMIPLLVLAGCTGSDGGSGSAEHISLEVTFPPEVQPGPQ
                     ATLVVTLYGYDPGFADASATVIQTDAVPLTATPGMFTIEFPTDAHERIDQGNGPVAFE
                     DARYYFVFYGDLDDDRQICAGELFQDHDNDGPYDFGPGAPPETVTIPMRIVDASFPCR
                     SAYPDGA"
     gene            2147..>3413
                     /locus_tag="VGB85_00170"
     CDS             2147..>3413
                     /locus_tag="VGB85_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0132447.1"
                     /translation="MILPMIKLAHSHRRAAGGALACALVCACTFNATGLGDDTGGNDG
                     SATSTGAPTTAGTMDATSTTDGTEGSGTDGTGTEGTTIDITTDPTTGVTTTTDPTTSP
                     TCGDGSVDPGEACDDGNEVNDDGCKSDCTLNICGDGIKGPSEGCDDGNMTPDDGCDPT
                     CSLETCGDGHKDPDEECDDGNIEDTDACANNCTTAKCGNGIQQADEECDEGAANSANA
                     MCTDACKIAKCGDGKTFEPMEECDDGNDEDKDACTTTCKHAKCGDGFEQPGEECDDGN
                     PENTDMCTTTCKDAKCGDGFLQGKEVCDDGNTSEDDACKNDCKPNGPLVFVTAAVYLG
                     DLGGMSGADGKCADAASAAMLPGTYKAWLSTDTEDAAGRLAHSNKPYYLTNGTKIAED
                     WNDLLSPPLLAAINYTETGAQAGNVTKVWT"
BASE COUNT          604 a         1182 c         1200 g          427 t
ORIGIN      
        1 tggcgcgctg gtccacggcg acctgctggg ccagaacctg cgcatcgact tcgacggcga
       61 gccgggcgtg atcgactggg agtacgccat gctcggcgat cccgcgcatg acctggcgat
      121 cgtcgcccgc ggagcccgcc ggccgtttca aagcgcggac ggccgggcca ggctgctgga
      181 ggcctacaat acgcgcgcga gcttcgaggt cacgaaggat gaccttcgct tcttcgagct
      241 cgcgctgcac gtgcggtggt tcaccggctg ccccgaccgc aaagaggatt cgcgcccgat
      301 cctcgcccgg ctgctcgcaa cgggatgagc accgcggcta gcccgtgaac gcgtcctcgt
      361 ggcgcatcag gacctcgtcg gcgcgcagcg acgcgatgac ccgggcatac tccgccagca
      421 ggctgcgcgc cgacacggcg tccgcggggt ctccgacgag cagatccttg cgggccgcgt
      481 cccacaggtc cgggctgcga tccgagcgga cggggatcgt cgcgcccttg cggtcgtact
      541 tgatcttctc catcttcgtg ttgggcaccc gcacccgctc gaggtcgacc ttgagctgcc
      601 cgacctccgc gaggaaccgc gccgcgaagc aggcgtgggc ctggcacatg gtgcgcgtca
      661 cctcgtaggc gccgagcgtc cacagccaca tcaggtccga cgcgcgcgcc cgcggccccg
      721 ccggcagggc ctcctcgccg ggggcgcagc ggtcgaggtt gtcgaggacc tcgtccgcga
      781 ccgacagcag gacctggttg cggcccgaca ggtagaacga aaacgcggtc agggggtgcg
      841 acgtgcgcat cgctcagccg accacggcga gggcggcacg cacggtcatc gccgcgagca
      901 ccagcgacga cacgaagaac agcgcgaagc gcagctcgat cttgttgaac gtcgcctgca
      961 ccaggctgtg gaccacgcgc gagccgacgt aggtccacgc gagcatcgca tcggtgtcga
     1021 cgccgcggct gacgagcgcg agcacgatcg ccagcgcgta gaacagcgtc ggctgctcga
     1081 acaggtggtt gtagttgtcg gccttccagc gcacctgcgc cggcaagctg gccatctgct
     1141 cgccgcgcgg cgccgtcggg tcgagccgca tgcccgagcg gtggatcgcc ggaatgcggg
     1201 tggcatacat ccagccccac atcacaaagg tccagacgac gagtgcgacg acggggcgaa
     1261 ggagctcgag ggatggttgc atggtaggtg cgacgatacc acagtccgtg cgccgcctcc
     1321 ggacctcacg gccctacgcg ccgtccgggt acgcggaccg gcacggaaag ctcgcgtcga
     1381 cgatccgcat cggtatcgtc accgtctctg gcggtgcgcc gggtccgaag tcgtaagggc
     1441 cgtcattgtc gtggtcctgg aacagctcgc cggcgcagat ctgccggtcg tcgtcgaggt
     1501 cgccgtagaa cacgaagtag tagcgcgcgt cctcgaaggc gaccggcccg ttgccctggt
     1561 cgatgcgctc gtgggcgtcc gtggggaatt cgatcgtgaa catgccgggc gtcgcggtga
     1621 gcggcacggc gtcggtctgg atcaccgtgg cggacgcgtc ggcgaagccc gggtcgtacc
     1681 cataaagcgt gaccaccagc gtggcctgcg gaccgggctg cacctccggc gggaaggtga
     1741 cctcgagcga gatgtgctcg gcgctcccgg agccgccgtc ggagcccgtg cagccggcga
     1801 gcacgagcag cgggatcatg agccaggcgt gacgcatgcc gacaggcgac cacaggcgca
     1861 cggtcctgtc gatacaccgc gacgtcgcgg atcgcggccg ccgtcgcctg atgacgactg
     1921 ccagtgaggt ggccgcggcc tcgcggtgcc gtccggctgg gtggtgagcc ccccggtcat
     1981 gcgcgcgagc acgcgcagga ccgcccgcgc tggccgcagt gcgctcgcgc gggcgccggg
     2041 cacccgcggg tttgcgcggc ctgccaaaga ggtcgacgcg gacgccccgc ctgggtgacg
     2101 atggcggtct ggttcctcga aaacgccggg gccggagcct gacaccgtga tcctacccat
     2161 gattaaactc gcccattctc atcgtcgcgc cgccggcggc gccctcgcct gcgccctcgt
     2221 ctgcgcgtgc accttcaacg cgaccggcct cggcgacgac accggaggca acgacggctc
     2281 ggcgaccagc accggcgcgc cgaccaccgc cgggaccatg gacgcgacgt ccaccacgga
     2341 cggcaccgag ggcagcggca ccgacggcac cggcaccgag ggcacgacga tcgacatcac
     2401 cactgacccg acgaccggtg tgacgaccac gaccgacccg acgacctcgc cgacgtgcgg
     2461 cgacggctcg gtcgatcccg gcgaggcctg cgacgacggc aacgaggtca acgacgacgg
     2521 ttgcaagagc gactgcacgc tcaacatctg cggcgacggc atcaaggggc cgagcgaggg
     2581 ctgcgacgac ggcaacatga cccccgacga cggctgcgac ccgacgtgca gcctcgagac
     2641 ctgcggcgac ggccacaagg accccgacga ggagtgcgac gacggcaaca tcgaagacac
     2701 cgacgcgtgc gccaacaact gcacgaccgc gaagtgtggc aacgggatcc agcaggcgga
     2761 cgaggagtgc gacgagggcg cggccaactc ggccaacgcg atgtgcaccg acgcctgcaa
     2821 gatcgcaaag tgcggcgacg gcaagacctt cgagccgatg gaggagtgcg acgacggcaa
     2881 cgacgaggac aaggacgcgt gcacgacgac gtgcaagcac gccaagtgcg gcgacggctt
     2941 cgagcagccc ggcgaggagt gcgacgacgg caaccccgag aacaccgaca tgtgcacgac
     3001 gacgtgcaag gacgccaagt gcggcgacgg cttcctgcag ggcaaggagg tgtgcgacga
     3061 cggcaacacg agtgaggacg acgcctgcaa gaacgactgc aagcccaacg gcccgctcgt
     3121 gttcgtcacc gccgcggtgt atctcggcga cctcggcggc atgtcagggg ccgacggcaa
     3181 gtgcgcggac gcggcgtcgg ccgcgatgct gcccggcact tacaaggcgt ggctgtcgac
     3241 cgacaccgag gacgccgccg gccgcttggc gcactcgaat aagccttact acctgaccaa
     3301 cggcaccaag atcgccgagg actggaacga cctgctcagc ccgccgctgc tcgcggcgat
     3361 caactacacc gagacgggcg cgcaggccgg aaatgtcacc aaggtgtgga cgg
//
LOCUS       DASWNJ010000007         3126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_494649, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000007 DASWNJ010000000
VERSION     DASWNJ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494649"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VGB85_00175"
     CDS             complement(<1..216)
                     /locus_tag="VGB85_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132448.1"
                     /translation="MTALATVTALALAGCVDKAPPPMWPAPPPPSVAEVIGAPAQSKA
                     PPMPVGVVGAPLLSGEPSPLDALPVDKA"
     gene            complement(282..845)
                     /gene="rpoZ"
                     /locus_tag="VGB85_00180"
     CDS             complement(282..845)
                     /gene="rpoZ"
                     /locus_tag="VGB85_00180"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEY0132449.1"
                     /translation="MARVTVEDCLEKIPNRFALTILAARRARALLENRGTPLVECDNK
                     GAVIALREIGNGSVRFQENVEAIMVDFIEEQRQKLKLTSSDNTFLEAAAFSVIEGEEA
                     DEEGDIIPELSADFEKVGLAPTETTDSDEPVDVEDADSEEEVVADDEEADLTAAADID
                     EDAAGLEDLDAGLEDDEADVAETEDEE"
     gene            complement(1000..1431)
                     /locus_tag="VGB85_00185"
     CDS             complement(1000..1431)
                     /locus_tag="VGB85_00185"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HEY0132450.1"
                     /translation="MLDFPIAELITPEEQVAVARAVAIMASRDNQVSDSEKHFVEELM
                     GQMMLLPEERDLVRKEFSTPHDLMQVARTVQHREARLFLFYQAVCAALADNKLVDGEM
                     DALLALAETFGFRPDHAKAFIRWVRDSLELRDRGQQLLVEM"
     gene            complement(1608..>3126)
                     /locus_tag="VGB85_00190"
     CDS             complement(1608..>3126)
                     /locus_tag="VGB85_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132451.1"
                     /translation="DETTGATEEPTTAEPFVPMPARGGIKIVRVEANPGVAVPIGLDG
                     APVGGAGRNAFIPGSRNLLIRAYVDLPEDWIERPIEARLQLVGGGVDKVMTQTVTLSS
                     DSVDADLKSTFYFGVEAVDVVPGLKYQVELWEAAAGAEDLPEPAEPAVALRDGPSFVG
                     VESDLAEMRVVLVPIDYSFGNCAAVVDGEAHRKDFEAGLYQQNAISALELTVHAPYKV
                     EYSMTEYSGLSKLVSAMSQLRTDEGADPNVYYYGLFDNCGGCIGAGDGLQAGCTVGLA
                     ADITGPDENDARFRAAAGQLNKGAVDTFVHEVGHTQGRRHIECAGAGVQAAGTDPSYP
                     YEDGRIGVWGFGIRDFGLRHPTVYSDYMSYCGRTWVSDWQWNATYTRIKKLTGWGKSG
                     AGAPATGDGVLIGAVDPDGQQIWWTGPGDLSSEREPSATHRVIFEFADGEVETTTQVS
                     VREHYATRNIVARLPEGFDERELIGVRWADEQGEQVVAPKLIRRLHKPVRLQTAQ"
BASE COUNT          410 a         1157 c         1078 g          481 t
ORIGIN      
        1 ggccttgtcg accggcaatg cgtcgagcgg gctcggctcg cccgacagca gcggcgcccc
       61 gaccacgccg accggcatcg gcggcgcctt cgactgcgcg ggcgcgccga tcacctcggc
      121 gacgctcggg ggcggcggcg ccggccacat cggcggcggc gccttgtcga cgcacccggc
      181 cagcgccagc gcggtcacgg tcgccagcgc ggtcacgatc gctgcggagg ctgctcgcag
      241 cccccccggc cgggtcgcac atgggccccg gcccgtcagc atcactcctc gtcctcggtc
      301 tcggcgacgt cggcctcgtc gtcctcgagg ccggcatcga gatcctcgag gccggcggcg
      361 tcctcgtcga tgtcggcggc ggcggtgagg tcggcctcct cgtcatcggc gacgacctcc
      421 tcctcggagt cggcgtcctc gacgtcgacg ggctcgtcgc tgtcggtcgt ctcggtcggg
      481 gccaggccca ccttctcgaa gtcggcggac agctcgggga tgatgtcccc ctcctcgtcg
      541 gcctcctcgc cctcgatgac gctgaaggcg gcggcctcga ggaaggtgtt gtcgctgctg
      601 gtcagcttca gcttttggcg ctgctcctcg atgaagtcga ccatgatggc ctcgacgttc
      661 tcctggaagc gcaccgagcc gttgccgatc tcgcggaggg cgatgaccgc ccccttgttg
      721 tcgcactcga ccaggggcgt accgcggttc tcgagcaggg cccgcgcgcg ccgggcggcg
      781 aggatcgtga gcgcaaaacg attggggatc ttctcgaggc agtcttcgac ggtgacgcga
      841 gccatgggcg gcggagtatg cgagcccgcg cgggcgggcg caagccccct gcagcctacc
      901 cgcaccggcg cagacggcgg ccgcggccgc cagaaacgca gaaaccgggc ccgaggtacc
      961 cccgagcccg gcgcgatccc ggcagcacgc cggtcctggc tacatctcca cgagcagctg
     1021 ctgcccgcgg tcgcgcagct cgaggctgtc acggacccag cggatgaacg ccttggcgtg
     1081 gtccgggcga aagccgaagg tctccgcgag ggccagcagg gcgtccatct cgccgtcgac
     1141 cagcttgttg tcggccagcg cggcgcacac cgcctggtag aacaggaaca gccgggcctc
     1201 gcggtgctgc accgtgcgcg cgacctgcat caggtcgtgc ggcgtcgaga actccttgcg
     1261 caccaggtcg cgctcctcgg gcagcagcat catctggccc atgagctcct cgacgaagtg
     1321 cttctcgctg tcggagacct ggttgtcccg ggaggccatg atcgccacgg cacgggccac
     1381 ggccacctgc tcttcggggg tgatgagctc tgcgatcgga aagtcgagca tcctggcttt
     1441 ctccagcttc gagcgccagg ctaacccaac tcggctgcgc tgcctagcgc cgcgtcgacc
     1501 ccaccggcgg cctggccccg ccggtccggc gcgcccctgc gaacgggtcc acgcgcaccg
     1561 gggagatccc ggtggcgcga caccgcatcg acatcagcgt caccgcgtca ctgcgcggtc
     1621 tgcaggcgaa cgggcttgtg gagccggcgg atcagcttgg gggcgacgac ttgctcgccc
     1681 tgctcgtcgg cccagcgcac gccgatcagc tcgcgctcgt cgaagccctc gggcagtcgg
     1741 gcgacgatgt tgcgggtcgc gtagtgctcg cgcacgctga cctgcgtggt cgtctcgacc
     1801 tcgccgtcgg cgaattcgaa gatcacgcgg tgcgtcgcgc tcggctcgcg ctcgctgctg
     1861 aggtcgccgg gcccggtcca ccagatctgc tggccgtccg gatcgaccgc gccgatcagc
     1921 accccgtcgc cggtggcggg cgcccccgcg ccgctcttgc cccagccggt cagcttcttg
     1981 atccgcgtgt aggtcgcgtt ccactgccag tcggacaccc aggtccgccc gcagtagctc
     2041 atgtagtccg agtacacggt cgggtggcgc aggccgaagt cgcggatgcc aaagccccac
     2101 acgccgatcc ggccgtcctc gtaggggtag ctcgggtcgg tgccggccgc ctgcacgccg
     2161 gcgccggcgc actcgatgtg ccggcgtccc tgcgtgtgcc cgacctcgtg cacgaaggtg
     2221 tcgacggcgc ccttgttgag ctggccggcg gcggcccgga agcgggcgtc gttctcgtcg
     2281 ggaccggtga tgtcggccgc gaggcccacg gtgcagccgg cctggagccc gtcgccggcg
     2341 ccgatgcagc cgccgcagtt gtcgaacagc ccgtagtaat agacgttcgg gtcggcgccc
     2401 tcgtccgtgc gcagctggct catcgccgac accagcttac tgaggcccga gtactcggtc
     2461 atgctgtact cgaccttgta cggcgcgtgg accgtcagct cgagcgccga gatcgcgttc
     2521 tgctgataca gcccggcctc gaagtccttg cgatgcgcct cgccgtcgac caccgccgcg
     2581 cagttgccga agctgtagtc gatcgggacc agcaccacgc gcatctccgc gaggtccgac
     2641 tcgacgccga cgaacgacgg cccgtcgcgc agcgcgaccg ccggctccgc gggctcgggc
     2701 aggtcctccg cgcccgcggc ggcctcccac agctcgacct gatacttgag ccccggcacg
     2761 acatcgacgg cctcgacccc gaagtagaag gtcgatttga ggtccgcgtc caccgagtcc
     2821 gagctgagcg taacggtctg cgtcatcacc ttgtcgaccc cgccgccgac cagctgcagg
     2881 cgcgcctcga tcggccgctc gatccagtcc tcgggcaggt cgacgtaggc gcggatcagc
     2941 aggttgcggc tgcccgggat gaaggcgttg cggccggccc cgcccaccgg cgcgccgtcg
     3001 aggccgatcg gcaccgcgac gcccgggttg gcctcgacac gcacgatctt gatcccgccg
     3061 cgggccggca tcggcacgaa gggctcggcc gtggtcggct cctcggtcgc cccggtcgtc
     3121 tcgtcg
//
LOCUS       DASWNJ010000008         4958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_388595, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000008 DASWNJ010000000
VERSION     DASWNJ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388595"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VGB85_00195"
     CDS             complement(<1..701)
                     /locus_tag="VGB85_00195"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0132452.1"
                     /translation="MPTVVVPDRLTSHVAAGHPWLYADGVPAVSGARTGALVTLLGQG
                     RRPIGQALYDADSPIALRVLTTRRGEQAGPELWQRRVAEALALRRGTLDLRTTDAYRL
                     LHGEGDRLPGVVVDHYAGFLVLKLDTPAWLPHLDDLRDTLMEVVRPRGIFFKGISGER
                     EAAAPRLLAGEDPPEHIAVREHGMRLLVDVRRGQKTGLFLDQRENRAVVRQVGRGRRV
                     LNLFSYTGGFSLAAAL"
     gene            882..3860
                     /locus_tag="VGB85_00200"
     CDS             882..3860
                     /locus_tag="VGB85_00200"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0132453.1"
                     /translation="MQAQKGAESGPVVAASPLDFGRMYLDEKAETFGYDVPAPSGSGP
                     APAGEPFRAGRYVILHHVGHGAMGNVYAAYDPDLDRKVAVKVLRDSTRGRPLPASLDR
                     EARAMAKLTHPHVVTVYDVGSCDRGVFVAMEFVDGVTLRTWLKAQVRSWREVLAAFVQ
                     AGQGLAAAHAAGVIHHDFKPDNVLVGAGGRVKVADFGLARVAASTAAAISGELIDVTR
                     SQEAGVAPPATGASLAASGSAAPTQANERGPVGTLAYMAPERHLARPADARSDQFSFC
                     VALYEALYQQRPFTGVDVAALRHAVLYDPPAEPPRATPVPVRVAAVLRRGLSRAPEDR
                     FATMDALLAALAEPAPRRLHWSLSGGLLLGAAVAMSAATRGEDRCQSATADEDQWWQR
                     QTGLLAARFPDAAVGQQLARLGQLQSNWAARKHDNCEAGQRGRLAPDLRRAREACLDQ
                     RMAQLRASAVQAGEASGAEEAVYLLAEVPATDGCDDSTLTNVPSPAPEQAPAVLRERQ
                     ALAVVAGLEVAGQYAAALRGLGPIERAAEALGYAPLLAEVLYQRARLEHYRGDSQRAR
                     ETLAQAIDHAEAHRHDRLAADAWGFLVEIAALATTSSERHTLEAWHRRARAAQARLHG
                     DRNDRADRLAIFLDEHGWTPWRTPRAGDPQQVHPLLMQGLVHLREGDAALAARSFDDA
                     LADADLHPRTRAKLLLNRAVAIQEQVGEDAAMRAWDEAVAAFATATWPGHPAHAEARV
                     RRGEFLQWKGRFAEARVDFAAALPALADVTSHAWALRTAHTGVAIADLMAFRVGSAAH
                     EVRAAVIASEMLVPDPMLASAGFEAELLAGAPQQARAAATREVALFQVDGDAMALARA
                     ETHLGEALAVLGDSQAARAQLNAALRRFTDAGAGAAEDTAYALKTLGLLALRERDELA
                     AEASLTRALALWEAQPCGCRDAAEARLGLGTLLSRRGDPRGPALRAEADAFFSPLGAE
                     AIAHRDRVVAWIGADVSLTN"
     gene            3885..4085
                     /locus_tag="VGB85_00205"
     CDS             3885..4085
                     /locus_tag="VGB85_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132454.1"
                     /translation="MNRNTVKLLLAALCLTTACETWPQQVPTHSNGDLSAPAKPMTDD
                     DCAGCYKWSSRPPSGINGPVLH"
     gene            4085..>4958
                     /locus_tag="VGB85_00210"
     CDS             4085..>4958
                     /locus_tag="VGB85_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0132455.1"
                     /translation="MSAASYIPYVRFRLADGTTAQVSPGGLIGRASSAELKIANPEVS
                     EAHALISLRGHQLHMLSLRRWIILDGERCSAIALRPGQRIHLSQTVVLEVEAVELPGH
                     VLVLACDDREVCTLSESVYSLSRTAAGLKVAPEHVLGAEAYLWGNGEGFTLRVGDASP
                     IEVEAGTEWQLGPHRLRVTARPLGDGALSTRSIGQNDPPLTIVARYDTVYIHTPGRAP
                     AVLSGLLARIVSELVTIGKLVSWEAVAREIWGDESDRNVMRQNWDRNMRRLRAHLREA
                     EVRADLVRPDGRGNV"
BASE COUNT          641 a         1799 c         1889 g          629 t
ORIGIN      
        1 agcgcggcgg ccagcgagaa gccgccggtg tacgagaaca ggttgagcac gcgacggccg
       61 cggccgacct ggcgcacgac ggcccggttc tcgcgctggt cgaggaacag gccggtcttt
      121 tgcccgcggc ggacgtcgac gagcaggcgc atgccgtgct cgcggacggc gatgtgttcg
      181 ggtggatcct cgccggcgag caggcgcggc gcggcggcct cgcgctcgcc gctgatccct
      241 ttaaaaaaga tgccgcgcgg gcggacgact tccatcagag tgtcgcgcag gtcgtcgagg
      301 tggggcagcc aggcgggggt gtcgagcttg agcacgagga agccggcgta gtggtcgacg
      361 acgacgccgg gcaggcggtc gccctcgccg tggagcaggc gatacgcgtc ggtggtgcgc
      421 aggtcgaggg tgccgcggcg cagcgcgagg gcctcggcga cgcggcgctg ccacagctcg
      481 gggccggcct gctcgccgcg gcgggtcgtc agcacgcgca gcgcgatcgg ggagtcggcg
      541 tcgtagaggg cctggccgat cggtcgccgg ccttgaccca gcagggtgac gagcgcgccg
      601 gtgcgcgcgc cggagaccgc gggcaccccg tcggcgtaca accacgggtg gccggcggcg
      661 acgtggctgg tcaggcggtc ggggacgacg acggtgggca cggccggagg gtacgtcatc
      721 gccccgggtc cgatgccaga gggaaggcga gccgcggacc tcgagctacg atgggttgct
      781 ctgctcatgc aggggtattc ccgccggtct gatcgaagtc cgatgtagtc tcgaaagttt
      841 ttctggtaac gtgggtttta gaaatgggag tttcaggaga tatgcaggcg caaaaaggcg
      901 ccgagtccgg tccggtggtg gcggcgagcc ccctggactt cgggcgcatg tatctcgacg
      961 agaaggcgga gaccttcggg tacgacgtgc cggcgccgtc cggttcgggg cctgcgcctg
     1021 caggtgagcc gttccgggcc ggtcgctacg tgatcttgca tcacgtcggg cacggggcga
     1081 tggggaacgt ctacgcggcg tacgacccgg acctcgaccg caaggtggcg gtcaaggtgc
     1141 tgcgcgactc gacgcgcggg cgcccgctgc cggcctcgct cgaccgcgag gcgcgggcga
     1201 tggccaagct gacccacccg cacgtcgtca ccgtgtacga cgtcggcagc tgcgaccgcg
     1261 gcgtgttcgt ggcgatggag ttcgtcgacg gggtgaccct tcggacatgg ctcaaggcgc
     1321 aggtccggag ctggcgcgag gtcctcgccg cgttcgtgca ggcggggcag gggctggcgg
     1381 cggcgcacgc ggcgggggtc attcatcacg acttcaagcc cgacaacgtg ctcgtcggcg
     1441 cgggcggtcg ggtcaaggtc gcggacttcg ggctcgcgcg ggtcgccgcg agcacggccg
     1501 cggcgatctc cggcgagctg atcgacgtca cgcgcagcca ggaggccggg gtcgcgccgc
     1561 cggcgacggg ggcttcgctg gcggcctcgg gttcggccgc gccgacgcag gccaacgagc
     1621 gcgggccggt cgggacgctc gcgtacatgg ccccggagcg gcacctcgcg cgtccggccg
     1681 acgcgcgctc ggatcagttc agcttctgcg tcgcgctgta cgaggcgctg tatcagcaac
     1741 gaccgttcac cggggtcgac gtcgcggcgc tgcgccacgc ggtgctgtac gacccgccgg
     1801 ccgagccccc gcgcgccacg ccggtgccgg tgcgcgtcgc ggcggtgctg cggcggggcc
     1861 tgtcgcgcgc gccggaagac cgcttcgcga cgatggacgc gctgctcgcc gcgctcgccg
     1921 agccggcgcc gcgccgcctg cactggtcgc tctcgggcgg gctgctgctc ggcgccgcgg
     1981 tggcgatgtc ggcggcgacc cgcggcgagg accgctgcca gtcggcgacc gccgacgagg
     2041 accagtggtg gcagcgccag accgggctgc tcgccgcgcg cttccccgac gccgcggtcg
     2101 gccagcagct cgcgcgcctc ggccagctgc agagcaactg ggccgcgcgc aagcacgaca
     2161 actgcgaggc cggccagcgc ggtcggctgg cgccggacct gcggcgcgcc cgcgaggcct
     2221 gcctcgacca gcgcatggcc cagctgcgcg ccagcgcggt ccaggccggc gaggcgagcg
     2281 gcgccgagga ggccgtgtac ctgctcgccg aggtgccggc gaccgacggc tgcgacgaca
     2341 gcaccctgac gaacgtcccc tcgccggcgc cggaacaggc tccggcggtg ctgcgcgagc
     2401 gccaggcgct ggcggtggtc gccgggctcg aggtcgcggg gcagtacgcg gcggcgctgc
     2461 gcggcctcgg gccgatcgag cgggccgcgg aggcgctggg ctacgccccg ctgctggccg
     2521 aggtgctgta ccagcgcgcg cgcctcgagc actaccgcgg cgactcgcag cgggcccgcg
     2581 agacgctcgc gcaggcgatc gaccacgccg aggcgcaccg ccacgaccgc ctggcggccg
     2641 atgcgtgggg cttcctcgtc gagatcgcgg cgctggcgac cacttcgagc gagcgtcaca
     2701 cgctcgaggc gtggcaccgc cgggcccgcg cggcccaggc ccgcctgcac ggcgaccgca
     2761 acgaccgcgc cgaccgcctg gcgatcttcc tcgacgagca cggctggacc ccgtggcgca
     2821 cgccgcgagc cggcgacccg cagcaggtcc accccttgct catgcaaggg ctcgtgcacc
     2881 tgcgcgaggg cgacgcggcg ctggcggccc gcagcttcga cgacgcgctc gccgacgccg
     2941 acctgcaccc gcgcacgcgc gccaagctgc tgctcaaccg cgccgtggcg atccaggagc
     3001 aggtcggcga ggacgcggcg atgcgcgcgt gggacgaggc ggtcgccgcg ttcgcgacgg
     3061 cgacctggcc aggacacccg gcgcacgcgg aggcgcgggt gcgccgcggc gagttcttgc
     3121 agtggaaggg ccgcttcgcg gaggcgcggg tcgacttcgc ggccgcgctg ccggccctcg
     3181 ccgacgtcac gagccacgcg tgggcgctgc gcacggccca caccggcgtg gcgatcgccg
     3241 acctgatggc gtttcgcgtc gggtcggccg cccacgaggt ccgcgccgcg gtgatcgcca
     3301 gcgagatgct ggtgcccgac ccgatgctcg ccagcgccgg cttcgaggcc gagctgctgg
     3361 ccggcgcgcc gcagcaggcc cgcgccgccg ccacccgcga ggtcgcgctg ttccaggtcg
     3421 acggcgacgc gatggccctc gcccgcgccg agacccacct cggcgaggcg ctggctgtcc
     3481 tcggggacag ccaggccgcg cgcgcgcagc taaacgccgc gctgcgccgc ttcaccgatg
     3541 ccggcgcggg cgcggccgag gacaccgcct acgcgctcaa gaccctcggc ctgctcgcgc
     3601 tgcgcgagcg cgacgagctc gcggccgagg cctcgctgac gcgggcgctc gccttgtggg
     3661 aagcgcagcc gtgcggctgc cgcgacgccg ccgaggcgcg cctcggcctc gggacgctgc
     3721 tgtcccgccg cggcgacccg cgcggtccgg ccctgcgggc cgaggccgac gccttcttct
     3781 cgccgctcgg cgccgaggcg atcgcccacc gcgatcgcgt cgtcgcatgg atcggcgcgg
     3841 atgtttcatt gacgaattga cccgaactgc ttggaggacc tgccatgaac cgcaacaccg
     3901 tgaagctcct gttggccgcg ctctgcctga ccaccgcctg cgaaacctgg ccgcagcagg
     3961 tccccacgca cagcaacggc gacctgtccg cgccggccaa gccgatgacg gacgacgact
     4021 gtgccggctg ttacaagtgg tcctcgcgtc cgccgtcggg gatcaacggc ccggtgctgc
     4081 attgatgtct gccgcgagct acataccgta cgtccgcttc cggctcgccg acggcacgac
     4141 cgcccaggtc tcgcccggcg ggctgatcgg acgggcttcg agcgcggagc tcaagatcgc
     4201 caaccccgag gtctcggagg cccacgcgct gatcagcctc cgcggtcacc agctgcacat
     4261 gctctcgctg cgccgctgga tcatcctcga cggtgagcgc tgctccgcga tcgcgctgcg
     4321 ccccggccag cggatccacc tgtcgcagac ggtcgtgctc gaggtcgagg ccgtcgagct
     4381 cccgggccac gtgctcgtgc tcgcgtgcga cgaccgcgag gtctgcacgc tctccgagtc
     4441 ggtgtactcg ctcagccgca ccgccgcggg cctcaaggtc gcgcccgagc acgtgctcgg
     4501 cgccgaggcc tatctgtggg gcaacggcga gggcttcacg ctgcgcgtcg gcgacgcgag
     4561 cccgatcgag gtcgaggccg gcaccgagtg gcagctcggc ccgcaccgcc tgcgcgtcac
     4621 cgcccgcccg ctcggcgacg gcgccctgag cacgcgcagc atcggacaaa acgacccgcc
     4681 gctgacgatc gtcgcccgct acgacacggt gtacatccat acgccggggc gcgcgccggc
     4741 ggtgctctcg ggcctgctgg cgcggatcgt cagcgagctg gtgacgatcg gcaagctggt
     4801 ctcgtgggag gccgtcgccc gcgagatctg gggcgacgag agcgaccgca acgtgatgcg
     4861 ccagaactgg gaccgcaaca tgcgccggct gcgggcccac ctgcgcgagg ccgaggtccg
     4921 cgcggacttg gtgcgccccg acggccgcgg caacgtcg
//
LOCUS       DASWNJ010000009         4350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_529949, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000009 DASWNJ010000000
VERSION     DASWNJ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529949"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..2878
                     /locus_tag="VGB85_00215"
     CDS             17..2878
                     /locus_tag="VGB85_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132456.1"
                     /translation="MLGTLLALADDRIPNQDAIALSPTIGWLLLGALLFVYLIFALQS
                     ERWRRFWLTVEDPRPIALFRIVFAFLCICNINDLWEYFTFLFTDEGLFLTDAARSQSA
                     GGQFKGFGEGQTGEPFGFFDAAGVLEYLKGPKFSLLYFFDTPRAMWAQMLAFYFVTVA
                     FMLGWRTRMMGILSFLLMNSFFVRNYLFWEGTELVYRVFFVYLLCARSGAAYSIDNWL
                     RCRRLRKQGLLSERDGPGGGAGMPPSEQHPRGLAAIYRLIPAWTRWLMVCNLGVLYCF
                     TGTVKNGHVWAKGDTLYYALNMDHFYRFYPQEVSSVLGLNLFRLATWVTHWWEAFFPL
                     MIVGLVTRWGLRERLEPLHGARLWAVRAAWVGLGLMVMAVVIVAFPVHYVPAKGGLNL
                     VSMQRVFAAGWLAMMAAIGGGWWLLGSRRMHVRIRGTQYVLDREWFCRWFLGRRVWLT
                     LGLMFHGQLLVLMNIGMFAPIMMSTYLFCLQGDEPGRILRFFGRGLARIGVPMPASLR
                     LHEPPLPAEDRRLPHHIRDGRKLPAWLLYGLIALAVLGVFLQAGPVVPWIKQHVLGRE
                     EAAAAGGLHFGWIALTIAVVLAVYTYVQGHRGTKNFVGKLVGLGAVLVAYLWLMGVRF
                     HDPQQAEILKFVRVGVNVGIALLLALQQVPAIHRRLAAIGLTFNPPDRPASEPETPVE
                     DPPGHARAPWAYRAGGRMLVGFLLVYHIQAIAVWEMPEKDSLSTFRLKAREPFTTWVF
                     STQTDQVWGMFAPNPPRHNVFMRVVLTDENGERWDMRTDVYAKERMPIPWIWNDRMRK
                     MNRRVIGGESGKGDVYQKWYGRYLCREWARTHRGVMPEKVELIKVSYKMPPPEAVAKQ
                     GWYDPHKLLHDTGREELQHVEKCATAIHGQLPDEIRERHGIPPLPEGAFVPLPNLRKK
                     AWDKRLEPAKAKTEAKTGTSAGKAGGAAKKATTKGTTTG"
     gene            3225..>4350
                     /locus_tag="VGB85_00220"
     CDS             3225..>4350
                     /locus_tag="VGB85_00220"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0132457.1"
                     /translation="MFRVPRAAGSEIPCAWRNFKKMMMKNLAYRGATVALSLSLAACG
                     DDGIAGTETGTSSGSTGQETTTGQPTTDTDPTTPTSTTTPTTTTDPTSEVTTDPTTGT
                     ATTVDTDTTPGTTLDTDGDTTTGTTDGTTGDTDSSGSSSTTDVMGVCGDGAIDAGEEC
                     DDGPDNGDMGACHADCKLDVCGDALVGPGEGCDAGPDNGDMNACKADCTPAVCGDALM
                     GPGEGCDDGNADDADECTNACALASCGDSIVSVSEECDDGNQEDTDLCTNACTEAECG
                     DTIVQPVAGEACDNGFENGDAGDCTATCNLATCGDGFIHNEGMGTEQCDNGGENGPGK
                     ACNAECMLNVCGDADLGPGEGCDDGNLVEGDGCNAGCALEV"
BASE COUNT          704 a         1404 c         1574 g          668 t
ORIGIN      
        1 cacgacactc ggggccatgc tcgggaccct cctggcgctc gcggacgatc gcatccccaa
       61 ccaggacgcg atcgcgctgt ctcccacgat cggctggctg ctcctcggag cgttgttgtt
      121 cgtctacctg atcttcgcgc tgcagagcga gcgctggcgc cggttctggc tgacggtcga
      181 ggacccgcgg ccgatcgcgc tgttccgtat tgtcttcgcg ttcctctgca tctgcaacat
      241 caacgacctg tgggagtact tcacgttcct gttcaccgac gaggggctgt tcctcaccga
      301 tgcggcccgc tcgcagtcgg ccggcggaca gttcaagggc ttcggcgagg gtcagaccgg
      361 cgagcccttc ggcttcttcg acgcggccgg cgtgctcgag tacctcaagg ggcccaagtt
      421 ctcgctgctg tacttcttcg acacgccgcg cgcgatgtgg gcgcagatgc tggcgttcta
      481 cttcgtgacc gtcgcgttca tgctcggctg gcgtacgcgc atgatgggca tcctcagctt
      541 tttgctgatg aacagcttct tcgtccgcaa ctacctgttc tgggagggca cggagctggt
      601 ctatcgcgtg ttcttcgtgt acctgctgtg cgcgcgcagc ggggcggcgt acagcatcga
      661 taattggtta cgctgccggc ggttgcgaaa gcaaggcttg ctcagcgagc gcgacgggcc
      721 cggcggcggc gccggcatgc caccttcgga gcagcacccg cgcggcctgg cggcgatcta
      781 ccgcctgatc ccggcgtgga cgcgctggct gatggtgtgc aacctcggcg tgctgtactg
      841 cttcaccggc acggtcaaaa atgggcacgt gtgggccaag ggcgacacgc tgtactacgc
      901 cctcaacatg gaccacttct atcggttcta tccgcaagaa gtgtcgtcgg tgctcgggct
      961 caatctcttt cgcctggcca cgtgggtgac ccactggtgg gaggcgttct tcccgctgat
     1021 gatcgtcggg ctggtgacgc gctgggggct gcgcgagcgc ctggaaccac tgcacggcgc
     1081 gcgcctgtgg gcggtgcgcg cggcctgggt cggcctcggg ctgatggtga tggcggtggt
     1141 gatcgtggcg ttcccggtcc actacgtgcc ggccaagggc ggcctcaacc tcgtcagtat
     1201 gcagcgcgtg ttcgcggccg gctggctggc gatgatggcg gcgatcggcg gcggctggtg
     1261 gctgctcggc agccggcgca tgcacgtgcg gatccgcggc acgcagtacg tgctcgaccg
     1321 cgagtggttc tgccggtggt tcctcggccg ccgcgtgtgg ctgaccctcg gcctgatgtt
     1381 ccacggccag ctgctggtgc tgatgaacat cggcatgttc gcgccgatca tgatgtcgac
     1441 gtacctgttc tgcctgcaag gggacgagcc gggccggatc ctgcgcttct tcgggcgagg
     1501 cctcgcgcgt atcggcgtgc cgatgccggc gagcctgcgg ctgcatgaac cgccgctgcc
     1561 ggccgaggac cgccggctgc cgcaccacat ccgcgacggc cgcaagcttc cggcctggct
     1621 gctgtacggg ctgatcgcgc tcgccgtgct cggcgtgttc ctgcaagccg gaccggtcgt
     1681 gccgtggatc aagcaacacg tgctcggccg cgaggaggca gcggcggccg gcggcctgca
     1741 cttcggctgg atcgcgctga cgatcgcggt ggtgctcgcc gtttacacgt atgtgcaggg
     1801 gcaccgcggc acgaagaact tcgtcggcaa gctcgtcggc ctcggcgcgg tgctcgtcgc
     1861 gtacctgtgg ctgatgggcg tgcgcttcca cgacccgcag caggccgaga tcctcaagtt
     1921 cgtgcgcgtc ggggtcaacg tgggcatcgc gctgctgctg gcgctgcagc aggtcccggc
     1981 gatccaccgc cgcctcgcgg cgatcggcct gaccttcaac ccgcccgaca ggcccgcgag
     2041 cgagccggag accccggtcg aggacccgcc aggccatgcc cgcgccccgt gggcctatcg
     2101 cgccggcggc cgcatgctgg tcggattttt gctcgtctat cacatccagg cgatcgcggt
     2161 gtgggagatg ccggagaagg actcgctgtc gacgttccgg ttgaaggcgc gcgagccgtt
     2221 cacgacgtgg gtgttctcga cgcagaccga ccaggtctgg gggatgttcg cgcccaatcc
     2281 tccacgccac aacgtattca tgcgcgtcgt gctcaccgac gagaacggcg agcgctggga
     2341 catgcgcacg gacgtgtacg cgaaagaacg catgccgatc ccgtggatct ggaacgaccg
     2401 catgcgcaag atgaaccgcc gcgtcatcgg cggcgagtcc ggcaagggcg atgtttatca
     2461 aaagtggtac ggcaggtacc tgtgccgcga gtgggcccgc acgcaccgcg gcgtgatgcc
     2521 ggagaaggtc gagctgatca aggtctctta taagatgccg ccacccgagg cggtcgcgaa
     2581 gcagggctgg tacgacccgc acaagctgct gcacgacacc ggccgcgagg agctgcaaca
     2641 cgtcgagaag tgcgcgaccg cgatccacgg ccagcttccc gacgagatcc gcgagcgaca
     2701 tgggatccca ccgctgcccg agggggcgtt cgtgccgctg ccgaacctgc gcaagaaggc
     2761 gtgggacaag cggctcgagc cggccaaggc caagacggag gcgaagaccg ggaccagcgc
     2821 ggggaaggct ggcggggccg cgaagaaggc gacgacgaag gggaccacga ccgggtagcg
     2881 gcggggcggc tatcaggtcg tcgaggtgta cgagtagcga ggtggtggag cgtgcgggcc
     2941 tgtcgcgaag gacatgtccg cacggccatg tcgctccccg agggccggcg tgggtctggc
     3001 tctcgactcc gtcggacgtc cggctactcg atcatgcgcg cgtccccagc cggacccacg
     3061 ccggcccgtg cgacgtcgtc cgcacggaca ctggcgacgt cgccctgtgc acctcgcggt
     3121 gatcatgcac ggccggccga cgcgccgagt gccgaagcga acgaagcgca gccgacgtcc
     3181 acaccgcaag cgcgacctac ggcccattac gcgcgctcgc gagcatgttt cgcgttccac
     3241 gcgcggcggg ctccgagata ccctgcgcgt ggaggaattt taaaaagatg atgatgaaga
     3301 acctggctta tcgcggcgca acggtggccc tatcgctgtc tctggcggcg tgcggcgacg
     3361 atgggatcgc aggcacggag acggggacga gcagcggcag caccgggcag gagacgacga
     3421 cggggcagcc gacgacggac accgatccaa cgacgccgac gagcacgacg accccgacga
     3481 cgacgacgga cccgaccagc gaggtgacga cggacccgac gacgggcacg gccacgacgg
     3541 tcgacacgga cacgacgccg ggcacgacgc tcgacaccga tggtgacacg accaccggga
     3601 cgacggatgg cacgaccggc gacacggaca gcagcggcag cagcagcacg accgacgtga
     3661 tgggcgtgtg cggcgacggt gcgatcgacg cgggcgagga gtgtgacgac gggccggaca
     3721 acggcgacat gggggcgtgc cacgcggact gcaagctcga tgtctgcggc gacgcgctgg
     3781 tcgggccggg cgagggttgt gacgccgggc cggacaacgg cgacatgaac gcgtgcaagg
     3841 ccgactgtac gcccgcggtg tgtggcgacg ctctgatggg tcccggggag ggttgcgacg
     3901 acggcaacgc ggacgacgcc gacgagtgca cgaacgcgtg cgcgctggcg agctgcggcg
     3961 acagcatcgt gtcggtcagc gaggagtgcg acgacggcaa ccaggaggac acggacctgt
     4021 gcacgaacgc ctgcacggag gcggagtgcg gcgacacgat cgtgcagccg gtcgcgggcg
     4081 aggcctgcga caacggcttc gagaacggcg acgcgggcga ctgcacggcg acctgcaacc
     4141 tggcgacctg cggcgacggg ttcatccaca acgaggggat gggcacggag cagtgtgaca
     4201 acggcggcga gaacgggccg ggcaaggcct gcaacgccga atgcatgctc aacgtctgcg
     4261 gcgacgcgga cctcgggccc ggcgagggct gcgacgacgg caacctcgtc gagggcgacg
     4321 gctgcaacgc cggctgcgcg ctcgaggtgt
//
LOCUS       DASWNJ010000010         4769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_636075, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000010 DASWNJ010000000
VERSION     DASWNJ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636075"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VGB85_00225"
     CDS             <1..469
                     /locus_tag="VGB85_00225"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEY0132458.1"
                     /translation="AGATAEIVSPEKRHVKGWKMTDWGDTFDVDVVLSKAKPEDYDAL
                     LLPGGVINPDLLRIDEDALRFIKHFVDGAKPIAAICHGPWTLIEVGAVAGRTMTSYSS
                     IKSDLKNAGAHWIDEAVVVDRGLVTSRNPGDLKAFNAKMIEEFAEGPHARQSA"
     gene            563..2065
                     /locus_tag="VGB85_00230"
     CDS             563..2065
                     /locus_tag="VGB85_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132459.1"
                     /translation="MVVTRPGAVALMLAATACGGEDGDSRADPSVASFASVGNTSEST
                     VDATSTTTGEQAPTTTGSGGASSSGSGDEPTTGALGTTGEASSSEGGESSSTGVMVGC
                     GDGVIDAPEVCDDANQVDGDGCNNDCTLSGQLLWESTQEGGVGLADEGIGCAIDGTGS
                     ILVTGYLSVGPGDDDLWVRKYSFSGVPVWTHTHPGTGLVKDQGRAVVVDVAELPYVAG
                     LTNTLMQDNDVFVRKFAADGMPMWTKAYSGPAMLADAGSAAALTPDGGLLVAGATTVV
                     DAGTNTWLRKYSPEGMTLWTRTYAGAAKGNDATHALAVTADGFIYAAGQEGVAGEGAN
                     VWLGKYDPDGNLIWSKIYSGAAGKNDYLYGAVAMDDGGVVVCGYETAIEIPWKSFLRR
                     YDAAGLAVWTEVDPGPEMLGALCYSVDLATNGDILFAGAVMQGTTREPRVRRLTPDGV
                     ELWSTVVPGAGEEVSQARCVKGAPDGTIVVTGSQDVGVDGRDIWVARFGP"
     gene            2134..2580
                     /locus_tag="VGB85_00235"
     CDS             2134..2580
                     /locus_tag="VGB85_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132460.1"
                     /translation="MLLALVASCSAPDIKPTSGTWTYTDIVQVSSTCAGEMSAPGGDF
                     TVTVLGEDRFTIGAEGLENPLDCNYGDNTFDCPETLLFKFTSDTDDSELIVTVEIEGT
                     LVSSTELEGREVIRQSCTGADCAKVIADGKLTLPCETTLSFTGLAK"
     gene            complement(2594..3481)
                     /locus_tag="VGB85_00240"
     CDS             complement(2594..3481)
                     /locus_tag="VGB85_00240"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0132461.1"
                     /translation="MDHTTRQIQSRDVRLHVEQWTPEGPPKFVVCMSHGAAEHMSRYA
                     RVAGWFAERGGLTFGADHRGQGSSGGPPGHVESFSVYAEDLHAVVNAEIEALPLESRP
                     DKIPWFLFGHSMGGLVALTYLLDHRDRQPFDGAIISAPLLGLTMKVPPLKLKVGQLAA
                     RLLPRLTLPSGLPASAISRDPAEVELYTRDTRRIDKVSARWFAAMNAAIVRVSAEAPQ
                     LDLPMLWYVGTGDRVCDPMATRALFDRFPEAGVRNQSLRSFDGYYHELHNEPELLRQP
                     IKEMLLGWIDQRMQAGKAA"
     gene            complement(3513..4685)
                     /locus_tag="VGB85_00245"
     CDS             complement(3513..4685)
                     /locus_tag="VGB85_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0132462.1"
                     /translation="MQMHRSADDLFNPSDEHRMLRRMVAEFARREVDPQAAEFDARGV
                     LNVALFRRLGDLGLLGITIPDEDGGAGLDTTAAVIVHHELAKYDPGFTLAYLAHSMLF
                     VNNFYYCSNAEQKARYLPKVLSGEWVAGMGMTEPGAGTDVLGMTTVAVQSGDEWILNG
                     TKTYITNGCEGHCFLVYAKVEGRVTAFVVDRDCPGFSTSNHIDKLGMRGSTMSELIFA
                     DCRVPAANLLGEVGSGLTHMMRNLEIERITLAAMSVGIAERCVEVMVRHAHERQAFKS
                     PLNRFGQIQRYIADGFAMTEAARCLVYNVARDIGPEIRNRVGSDAAKLFAAPVGKTVA
                     DYAIQVMGGAGYCKEFPVERLWRDAKLLEIGGGTIEAHQKNLTKDLTAFYLSSSGS"
BASE COUNT          745 a         1528 c         1775 g          721 t
ORIGIN      
        1 ggccggcgcg accgcggaga tcgtgtcgcc ggagaagcgg cacgtgaagg ggtggaagat
       61 gaccgactgg ggcgacacct tcgatgtcga cgtcgtgctc tcgaaggcga agcccgagga
      121 ctatgacgcg ctgctgctgc cgggtggcgt gatcaacccc gacctgctgc gcatcgacga
      181 ggacgcgctg cgctttatca agcacttcgt cgatggcgcc aagccgatcg cggcgatctg
      241 ccacggccca tggaccttga tcgaggtcgg cgcggtggcc ggacgcacga tgacctcgta
      301 cagctcgatc aagtccgacc tcaagaacgc gggcgcgcac tggatcgacg aggcggtggt
      361 ggtcgaccgc ggcctggtca ccagccgcaa ccccggcgac ctcaaggcct tcaacgcgaa
      421 gatgatcgag gagttcgccg aggggccgca cgctcgtcag agcgcatgat cggtggatcg
      481 gtggatcggt ggatcggtgc ggtctcggcc gtggcgccga ttgcgagggt gcgtcgcggc
      541 gggcgcccgg gtacgatggg ccatggtcgt gacacgtccg ggcgccgtgg ccctcatgct
      601 cgccgcgacg gcgtgtggcg gcgaagatgg cgacagccgg gccgacccct cggtcgcgtc
      661 gttcgccagc gtcggcaaca cgagcgagtc gaccgtggac gcgacgagca cgaccacggg
      721 ggagcaggcg ccgacgacca ccgggtccgg cggcgcgagc agctcgggca gtggcgacga
      781 gccgaccacg ggcgcgctcg gtacgaccgg cgaggcgtcg agcagcgagg gcggcgagtc
      841 ctcgagcacg ggcgtgatgg tcggctgcgg cgacggtgtg atcgacgcgc cggaggtctg
      901 tgacgacgcc aaccaggtcg acggcgacgg ctgcaacaac gactgcacgc tctcggggca
      961 gctgctgtgg gagtcgacgc aggagggcgg ggtcggcctc gcggacgagg gcatcggctg
     1021 cgcgatcgat ggcaccggat cgatcctcgt gaccgggtac ctgagcgtcg gtccgggtga
     1081 tgacgatctg tgggtgcgca agtactcgtt ctccggggtc ccggtatgga cgcacaccca
     1141 ccccgggacc gggctggtca aggatcaggg gcgcgcggtc gtcgtcgacg tcgccgagct
     1201 gccgtacgtc gccggcctca ccaacacgct gatgcaggac aacgacgtgt tcgtgcgcaa
     1261 gttcgcggcc gacggcatgc cgatgtggac caaggcgtac agcgggccgg cgatgctcgc
     1321 ggacgccggc agcgcggccg ccctgacgcc cgacggcggc ctgctggtcg cgggcgcgac
     1381 cacggtggtc gacgccggca ccaacacgtg gctgcgcaag tactcgcccg agggcatgac
     1441 cctgtggacg cggacctacg cgggcgcggc gaagggcaac gacgcgaccc acgcgctcgc
     1501 ggtgacggcc gatggtttca tctacgcggc ggggcaagag ggtgtcgctg gcgagggggc
     1561 caatgtctgg ctcggcaagt acgacccgga cggcaacctg atctggtcga agatctacag
     1621 cggcgctgcg ggcaagaacg actacctgta cggcgcggtc gccatggacg acggcggcgt
     1681 ggtcgtgtgc ggctacgaga cggcgatcga gatcccatgg aagagcttct tgcgccgcta
     1741 cgacgcggcc gggctggcgg tgtggaccga ggtcgacccg ggccccgaga tgctcggcgc
     1801 gctgtgttac agcgtcgacc tcgcgaccaa cggcgacatc ctgttcgcgg gcgcggtgat
     1861 gcagggcacc acgcgtgagc cgcgggtgcg ccgcctgacg cccgacggcg tcgagctgtg
     1921 gtcgacggtc gtgccgggcg cgggcgagga ggtcagtcag gcccgctgcg tcaagggggc
     1981 gccggacggc acgatcgtgg tcacgggttc gcaggacgtc ggtgtcgatg gtcgcgatat
     2041 ctgggtcgcg cgcttcggcc cctgagtgac tgtggttgcg ggtcggccac agccgtgctt
     2101 gcatggctca tgcgccgcca cgcttcacgt ctgttgctcc tcgcgctggt cgccagctgc
     2161 tctgcgcccg acatcaagcc gacgtccggc acgtggacgt acaccgatat cgtccaggtc
     2221 agctcgacct gcgccggaga gatgagcgcg cccggtgggg acttcacggt gaccgtgctc
     2281 ggggaggacc gcttcacgat cggggccgag gggctcgaaa atccgctcga ctgcaactac
     2341 ggggacaaca ccttcgactg tccggagacg ctgctgttca agttcacgtc ggacacggac
     2401 gactcggagc tgatcgtcac ggtcgagatc gaaggcacgc tggtgagctc gaccgagctc
     2461 gaggggcgcg aggtcatccg ccagagctgc accggggccg actgcgccaa ggtgatcgcc
     2521 gacggcaagc tgacgctgcc ctgcgagacg acgctgtcct tcaccggcct ggcgaagtga
     2581 gtcgcgcggc gcctcaggcg gccttgccgg cctgcatgcg ctgatcgatc cagccgagca
     2641 gcatctcctt gatcggctgg cgcagcagct ccggctcgtt gtgcagctcg tgataataac
     2701 cgtcgaagct gcgcaggctc tggttgcgga cgccggcctc ggggaagcgg tcgaacagcg
     2761 cgcgggtggc catcgggtcg cacacgcggt cgccggtgcc gacgtaccac agcatcggca
     2821 ggtcgagctg cggcgcctcg gcggagacgc ggacgatcgc ggcgttcatc gcggcgaacc
     2881 agcgcgcgga gaccttgtcg atccgccgcg tgtcgcgggt gtacagctcg acctccgccg
     2941 ggtcgcgcga gatcgccgag gccggtagcc cgctcggcag cgtcaggcgc ggcaggaggc
     3001 gcgcggcgag ctggccgacc ttgagcttga gcggcgggac cttcatcgtc aggccgagca
     3061 gcggcgccga aataatcgcg ccgtcgaaag gctgacggtc gcgatggtcg agcaggtagg
     3121 tgagggcgac caggccgccc atgctgtgcc cgaacaggaa ccacgggatc ttgtcggggc
     3181 gcgactcgag cgggagcgcc tcgatctcgg cgttgacgac ggcgtgcagg tcctcggcgt
     3241 agaccgagaa cgactcgacg tggccgggcg ggccgcccga cgaaccctgt ccgcggtggt
     3301 cggcgccgaa ggtcaggccg ccgcgctcgg cgaaccagcc ggcgacgcgg gcgtaacgcg
     3361 acatgtgctc ggcggcgccg tgggacatgc agacgacgaa tttgggcggg ccctcggggg
     3421 tccactgttc gacgtgcaaa cggacatcgc ggctctggat ctgcctcgtc gtgtggtcca
     3481 cgcgcgtatt agaccacaac ttcgcgccgc ggtcaggacc cgctggagga caggtagaag
     3541 gcggtcaggt ccttggtgag gttcttttgg tgggcctcga tcgtgccgcc accgatctcc
     3601 agcagcttgg cgtcgcgcca cagtcgctcg accgggaatt ccttgcagta gccggcgccg
     3661 cccatcacct ggatcgcgta gtcggcgacc gtcttgccga cgggcgcggc gaacagcttg
     3721 gcggcgtcgg agccgacgcg gttgcggatc tccgggccga tgtcgcgggc gacgttgtag
     3781 accaggcagc gggcagcctc ggtcatggcg aagccgtcgg cgatgtagcg ctggatctgg
     3841 ccgaagcggt tgagcgggct cttgaacgcc tgccgctcgt gggcgtggcg gaccatgacc
     3901 tcgacgcagc gctcggcgat gccgacgctc atcgccgcga gcgtgatgcg ctcgatctcg
     3961 aggttgcgca tcatgtgcgt caaaccactg ccgacctcgc ccagcaggtt ggccgccggc
     4021 acccgacagt cggcgaagat cagctccgac atcgtcgagc cgcgcatgcc gagcttgtcg
     4081 atgtgattgc tggtcgagaa gcccgggcag tcgcggtcga ccacgaaggc cgtcacccga
     4141 ccctcgacct tggcgtacac gaggaagcag tgaccctcgc agccgttggt gatgtaggtc
     4201 ttggtgccgt tgaggatcca ctcgtcgcca ctctggaccg caacggtcgt catgccgagc
     4261 acgtcggtgc cggcgcccgg ctccgtcatg cccatgcccg cgacccactc gccgctgagc
     4321 accttcggca ggtagcgcgc cttctgttcg gcgttcgagc agtagtaaaa attgttcacg
     4381 aacagcatcg agtgagcgag gtacgccagc gtgaagccgg ggtcgtactt ggccagctcg
     4441 tgatgcacga tcaccgcggc ggtggtgtcg aggccggcgc cgccgtcctc gtcggggatc
     4501 gtgatgccga gcaggccgag gtcgccgagg cgacgaaaca gcgcgacgtt gagcacgccc
     4561 ctggcgtcga attcggcggc ctgcggatcg acctcgcggc gcgcgaactc ggcgaccatg
     4621 cggcggagca tgcggtgctc gtcgctggga ttgaacaggt cgtcggccga gcggtgcatc
     4681 tgcatgagcg tggcacttct gggccgtggg ccgggagccc ctcgcgcgtg tgctggctca
     4741 agcgaggcgc gggggttccc ggcgagagc
//
LOCUS       DASWNJ010000011         2618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_176825, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000011 DASWNJ010000000
VERSION     DASWNJ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176825"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VGB85_00250"
     CDS             complement(<1..612)
                     /locus_tag="VGB85_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132463.1"
                     /translation="MRASLPGVLAGFMVGSLALGGCTRSGTQEAAQVVADPALQGLLQ
                     HVPADTPYAFISMGGGGMREFMAKLYGPLAPLMKQAEGTLASADLQRELGLSDEKYAL
                     LKAVLDEFKGKLSVEGMAELGIEVDARFAFYGIGMLPAMRWQLRDPAVLRATIERIQS
                     KSGTRFPVSKLGEVEYWQITGDGYEGAVAIVGDQLVAGMAPAAQ"
     gene            complement(716..1420)
                     /locus_tag="VGB85_00255"
     CDS             complement(716..1420)
                     /locus_tag="VGB85_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEY0132464.1"
                     /translation="MNDFLAELTGTMMLILLGDGVVAGTLLRGSKSEGAGWIVITVGW
                     ALAVTFAVYAAGGISGGHLNPAVTLALAVAGEFAWDRVPQYMLAQLLGAFLGACLVWL
                     HYLPHWKPTADPGLKLAVFCTGPAIRHAPGNFLSEALGTFVLLFGLSALGAHQFAEGL
                     NPLAVGALVLAIGLSLGGTTGYAINPARDLGPRLAHQLLPIAGKGGSDWRYAWIPVVA
                     PLVGGVLGALAHRALF"
     gene            complement(1428..2108)
                     /locus_tag="VGB85_00260"
     CDS             complement(1428..2108)
                     /locus_tag="VGB85_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132465.1"
                     /translation="MKHLACTLSLGLLVACGAGKSGEDTGTGAAGTSTTGATTDAPTS
                     SSSTGGPTSAAPTTTSSTGTPDLTSTGDESCGTACTSEGGNFLPGEPDAGDKLACDVL
                     AEDCPAGQKCMPYVDGGDALWNALKCVPVDPAPVGVGEACDAPGNGLTGVDNCDKHVM
                     CWDVENDMGVCAAMCVDDGCAEGFNCFVANDGILNLCLQACDVNMPDCPPGTGCLPVV
                     DGGVCAPA"
     gene            2264..>2618
                     /locus_tag="VGB85_00265"
     CDS             2264..>2618
                     /locus_tag="VGB85_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0132466.1"
                     /translation="MNFSDLGLLPSLVRAVTDAGYTEPTPIQVEAIPPVLAGRDVVGC
                     ARTGTGKTAAFALPILQRIDAMAGDDPKLRALILTPTRELAAQIEDSFSKYGKHMEMW
                     TAVIFGGVGQGPQEKE"
BASE COUNT          399 a          929 c          930 g          360 t
ORIGIN      
        1 ctgggccgcc ggggccatgc ccgcgaccag ctggtcgccg acgatcgcca cggcgccctc
       61 gtaaccgtcg ccggtgatct gccagtactc gacctcgccg agcttgctga ccgggaagcg
      121 cgtgccggac ttgctctgga tgcgctcgat cgtggcgcgc agcaccgcgg ggtcgcgcaa
      181 ttgccagcgc atcgccggca gcatgccgat gccgtagaac gcgaagcggg cgtcgacctc
      241 gatgccgagc tcggccatgc cctcgaccga gagcttgccc ttgaactcgt cgagcaccgc
      301 cttgagcagc gcatacttct cgtcggacag gccgagctcg cgctggaggt cggcgctggc
      361 gagcgtgccc tcggcctgct tcatcagcgg cgcgagcggc ccgtagagct tcgccatgaa
      421 ctcgcgcatg ccgccgccgc ccatgctgat gaacgcgtag ggcgtgtcgg ccgggacgtg
      481 ctggagcagc ccctggaggg cagggtcggc gaccacctgc gcggcctcct gcgtgcccga
      541 gcgcgtacac ccgccgaggg ccagcgaacc gaccatgaac cccgccagca cacccgggag
      601 cgacgcacgc aaccatcgtc tacgcataac ttctgtctcc tgctgccgcg gatgatacac
      661 aaaaacctcc gcgggttcgc aatgaacggg cgacggcccg cgagcattcc gttttttaga
      721 acagcgcccg gtgggcgagg gcgccgagca ccccgccgac gagcggcgcg accacgggga
      781 tccacgcgta gcgccagtcg gagccgccct tgccggcgat cggcagcagc tggtgggcca
      841 ggcgcgggcc gaggtcgcgg gcggggttga tcgcgtagcc ggtggtgccg ccgagcgaga
      901 ggccgatcgc cagcacgagc gcgccgacgg ccaggggatt gaggccctcg gcgaactgat
      961 gcgcgccgag tgccgacagg ccgaacaaca gcacgaaggt gccgagcgcc tcgctgagga
     1021 agttgccggg cgcgtgacgg atcgcagggc cggtgcagaa caccgcgagc ttgagccccg
     1081 ggtcggcggt cggcttccag tgcgggaggt agtgcagcca caccaggcag gcgccgagga
     1141 acgcgccgag cagctgcgcg agcatgtact gcggcacgcg gtcccaggcg aattcgccgg
     1201 cgaccgcgag cgcgagcgtc accgccgggt tgaggtgccc gccgctgatc ccaccggccg
     1261 cgtacacggc gaaggtgacg gcgagcgccc agccgaccgt gatcacgatc cagccggcgc
     1321 cctcgctctt gctgccgcgc agcagcgtgc cggcgaccac gccgtcgccg agcaggatca
     1381 gcatcatcgt gccggtgagc tcggcgagga agtcgttcat gacgcgctca cgcgggggca
     1441 catacgccgc cgtcgaccac cggcaggcag ccggtgccgg gcgggcagtc gggcatgttc
     1501 acgtcgcagg cctgcaggca caggttgagg atcccgtcgt tggcgacgaa gcagttgaag
     1561 ccctcggcgc agccgtcgtc gacgcacatc gccgcgcaca cgcccatgtc gttctcgacg
     1621 tcccagcaca tcacgtgctt gtcgcagttg tcgaccccgg tcaggccgtt gcccggcgcg
     1681 tcgcaggcct cgccgacgcc gaccggcgcc gggtcgaccg gcacgcactt gagggcgttc
     1741 cacagcgcgt cgccgccgtc gacgtagggc atgcactttt gtccggccgg gcagtcctcg
     1801 gccagcacgt cgcacgcgag cttgtcgccg gcgtcgggct cgcccgggag gaagttgccg
     1861 ccctcgctgg tgcacgcggt gccgcagctc tcgtcgccgg tgctcgtgag atcgggcgtg
     1921 ccggtcgagg aggtcgtggt cggcgccgcg ctggtcgggc ctccagtgct ggagctcgag
     1981 gtcggcgcgt cggtggtcgc gccggtcgta ctggtgccag cagccccggt gccggtgtcc
     2041 tcgccggact tcccggcgcc acaagcgacc agcaggccca gcgagagggt gcaggcgagg
     2101 tgcttcacgg cgcgacggta acaaggtcgc gcgtgaccca caacctccgg cggtccattc
     2161 gcgtgaattc ccacacggtc gaatcgcggc cggttcacct ccatggggcc gttgtggcgg
     2221 gaagccgctc gacgggcggc cgggaatgtg tcaggcacgg cgcgtgaact tcagcgacct
     2281 ggggctcctc ccgagccttg tccgcgccgt gaccgacgcg ggctacaccg aacccactcc
     2341 cattcaagtc gaggccatcc cgccggtgct cgccggccgc gacgtcgtcg gctgtgcgcg
     2401 caccggcacc ggcaagacgg cggccttcgc cctcccgatc ctccagcgca tcgacgccat
     2461 ggccggcgac gacccgaagc tgcgcgcgtt gatcctgaca cccacgcgcg agctggccgc
     2521 gcagatcgag gacagcttct ccaagtacgg caagcacatg gagatgtgga ccgccgtgat
     2581 cttcggcggc gtcggccagg gcccgcagga gaaggaga
//
LOCUS       DASWNJ010000012         8208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_565446, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000012 DASWNJ010000000
VERSION     DASWNJ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 8208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8208
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565446"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..3908
                     /locus_tag="VGB85_00270"
     CDS             30..3908
                     /locus_tag="VGB85_00270"
                     /inference="COORDINATES: protein motif:HMM:NF020076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEY0132467.1"
                     /translation="MTMLHARRFLSSCLSLAMVTACNPGTPASTAETPAEKTSGPGAT
                     APTPADAPVNDPVAELTAVHGPAGARTENLAEGTFLGAGAKLRASEVLLVPRGTLAEL
                     RLMDGTIVRVNEDSRVLLPDGPRSRNLELRSGELVALVAGGQQPLTIKAGEDTLEVTA
                     GEAHALAREGKHSYDVVYGAAVLRSGGQNVSLTPGAHVETPLTPAQVAARPVEPEISL
                     RPLEDTAWSRTFEIASAMADAVPPGVGSLTARRAGTRTEMHKLALTDQKVNVAISGRI
                     ARTEVEQTFYNEAGEVLEGTYRFPLPADASISGLSLLVGDVWMNAEMLEKERAREIFR
                     QIVDATVPRDPALLEWEKGNIFKMKIFPIPGRGERKIRISYTQVLPTIGDTLRYRYPI
                     AGASSGATGDEIGNFDFTVAIDKAELPPDGLAAIATPMAQLDRRELPERVELSTHKQR
                     FRPVYDLGVDIPLTKTDQRQHSETHLDRDGQAYFMLALKPDLKLPASDRPTHYAFVLD
                     RSHSMTPELWTVARSILQATTTALGPDDRMTVLACDAACDAAPGGLAPAAQAMSGVDT
                     FLDQQILAGASDLGGMMRSAAEALHGTDAGATAPQGPDEPERIIVYLGDGNPTAGALA
                     PDELLRHLEKPLAGTRVQAVALGARSDLLFLDALTRRTGGDLLRADAKDDLRGLVREL
                     RLRAEVPVARDVQLTLPRGMEYVHPQKLTALRPGDTVLLVGKLSAAVHGALEVQATGP
                     SGQVVKDSFQVDLEATPSAEGARHTHLPRTWARFEIDDLTATKGHAARAEIIDLSQQY
                     NVLSRYTALLVLENDAMFREFNVVRQAGKTTGWNGQLPTGTGTAPGQIASKDPAPAAG
                     APAPVAPPPPVASPPSPEPADAGRANEAPRAPAPKEAKGEAAADDNRRADRDVELQEV
                     QDPASREQPPEEEPPVEKLDGLDDALASSPERDAPADSKAPSAKPAPSPAKAKRSEAA
                     DPMSDSPGSSVGGYGSGGLASPGRRAPAQWRINAAGPPAPDVLAKLETMRQAVLRDPT
                     SRPDHRKLVQSAIRAGAPEAFTYATAWSQADPDHAPALLAVADLLSARSDPAAMRAYG
                     SAVEVNPFNLKLQTRLADAYAGKGDITRSCAHRRAVVSIEPAKADNHLQLARCLATLG
                     RQDLVLDAVGDGLGRAKGNLAELRASQSGLRPARPRPVTGNLKATLTWNGAGDLDVAF
                     IDNRGRRLSALRPEGVTVEQLGNGETAGLASVSGTVSVEVTRFSGQGPVSGELKVRTP
                     ETTRVFPFTIDQGTLRLANVTFTDYYRGGW"
     gene            3929..4909
                     /locus_tag="VGB85_00275"
     CDS             3929..4909
                     /locus_tag="VGB85_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132468.1"
                     /translation="MSLRTGVLAALLPAVFAAVFGACASAPATPTTGVVIDPEAPQVT
                     PHGDLGILRHRAAIGLTADVRAELAPRLAAVADRPGPDPGRDALRALAIELALIQGDA
                     DAARAELDRLDRTIAGQGERASAELRALAATLRGAWLFDAHRYADARSSHLRALVALD
                     QQPPDPQGAAQKGAALRALARDQLALGEAEAAVNTLGKAIELHRDSPEAHIELHEDLL
                     LAVDVMIAVQQPDEAVIVSSDAYNQALSKFGSDTLPHAEALLLVGAATLAKGDRSAAA
                     SVISDARAILDKLQSERSDPRFPISARALRRSDDLMQALAASPTEKTTSI"
     gene            5180..6307
                     /locus_tag="VGB85_00280"
     CDS             5180..6307
                     /locus_tag="VGB85_00280"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEY0132469.1"
                     /translation="MAGTSSNSTVHAPEHERLPSAPAGRGLLGFLRDFHNKTILFRVG
                     DWIFVTYGLIAGIAFFFGFSTTVWYLAMIGQDPAVTAAVCLFFVIPSVLMVSRVTSIL
                     LEWRELFRRPLQTLLKPGYMLHGGIFGGVLAMAGYAMAGGPSLPQLLDGLGFAMPLGE
                     AICRLGCFVYGCCWGKPTNSRFGVRYTSKHSKVIRFQPELQGVKIHPTQIYALTAHLI
                     QFTIFYALLPYKAFDGMFAALYLITHPMIRFVLETFRQDDRGKLGKLTHSNIYSLMMM
                     GIGLLVLAYGMSSAGTNLPIDVHFRYIHTIADGEALAYCTLTFFVAAAAFGVHYRNVG
                     SWLSKPSGGVAAQVDELSLGASARDDGLHDDNCESCDHHER"
     gene            6311..8014
                     /locus_tag="VGB85_00285"
     CDS             6311..8014
                     /locus_tag="VGB85_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEY0132470.1"
                     /translation="MSDITPELHDLAAEVARRRTFAIISHPDAGKTTLTEKLLLYSGA
                     IQLAGAVRAKKAGRATTSDWMEMERERGISVTTSVMSFEYPHPELPADAPPVQVNLLD
                     TPGHADFGEDTYRVLTAVDAALMVIDGAKGVEERTEKLIEICRLRDTPVVTFINKYDR
                     ECLDPLELMGDVEKKLGMTCVPLTWPVGMGKRFKGVYDLQRHRMHMFAAQGHHKIQEG
                     ILVEGLDDPRLDELLGDQVDELRMAAELLGGASQPLDRAAFLAGKQTPLLFGSAMNNF
                     GVRELLEAYIRLAPGPLSRQAITGSAVTAPPAPGEAVPVRTVLPTEEAFTGFVFKIQA
                     NMDPQHRDRMAFMRVCSGRFERGMRAFHCRLGREVGLGNATTFLARDREIVETAYAGD
                     IIGLPNHGTIKIGDAFTDGESLRFTGIPSFAPELFRRVLLKSPLKAKSLAKGLTQLAE
                     EGAIQVFKPFIGSQFIVGAVGQLQLEVMKHRLKSEYDVDADYEGVEYSTARWITPKTE
                     GKSKIDVQKLMENFQTRNQQNLATDSHGDLAYLAPNRWNLQKVEERFPDIRFAATREH
                     T"
     gene            complement(8019..>8208)
                     /locus_tag="VGB85_00290"
     CDS             complement(8019..>8208)
                     /locus_tag="VGB85_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132471.1"
                     /translation="YRYDLCWDTHAVQRESLPAPAYFEGCLAEAGWRGRLECLRAAPF
                     TAPIETCIAGTDYASEQC"
BASE COUNT         1272 a         3180 c         2678 g         1078 t
ORIGIN      
        1 gaggcgttct cccgccgccg gatctcccca tgaccatgct gcacgcgcga cgcttcctgt
       61 cctcctgcct ctccctggcc atggtgaccg cctgcaaccc cggcacgccg gccagcaccg
      121 cggagactcc ggccgaaaaa acctccggcc cgggtgcgac cgccccgacc cccgccgacg
      181 cccccgtcaa cgaccccgtc gccgagctga ccgccgtgca cggccccgcc ggcgcgcgca
      241 ccgagaacct ggccgaaggg acatttttgg gcgccggcgc caagctgcgc gccagcgagg
      301 tcctgctggt cccccggggc accctcgccg agctgcgcct gatggacggc accatcgtgc
      361 gcgtcaacga ggacagccgc gtccttttgc ccgacggccc gcgcagccgg aacctcgagc
      421 tgcgcagcgg cgaattggtc gcgctggtcg ccggtggcca gcagccgctg acgatcaagg
      481 ccggcgagga caccctcgag gtcaccgcag gtgaggccca cgcgctcgcc cgcgagggca
      541 agcacagcta cgacgtcgtc tacggcgcgg cggtcctgcg cagcggcgga cagaacgtct
      601 cgctgacccc cggcgcgcac gtcgagaccc cgctgacgcc ggcccaggtc gccgcgcggc
      661 cggtcgagcc ggagatcagc ctgcgcccgc tcgaggacac cgcctggtcg cggaccttcg
      721 agatcgcctc cgcgatggcg gacgccgtgc cgcccggcgt cggcagcctg acggcccgcc
      781 gcgccggcac ccgcaccgag atgcacaagc tcgcgctcac cgatcaaaag gtgaacgtcg
      841 cgatctccgg gcggatcgct cgcaccgagg tcgagcagac cttttataac gaggccggcg
      901 aggtgctcga gggcacctac cggttcccgc tgccggccga cgcctcgatc tcgggcctgt
      961 cgctgctcgt cggcgacgta tggatgaacg ccgagatgct cgagaaggag cgcgcccgcg
     1021 agatcttccg ccagatcgtc gacgcgaccg tcccgcgcga cccggcgctg ctcgagtggg
     1081 agaagggcaa cattttcaaa atgaagatct tcccgatccc cgggcgcggg gagcggaaga
     1141 tccggatctc gtacacgcag gtgctgccga cgatcggcga caccctgcgg taccgctatc
     1201 cgatcgccgg ggccagcagc ggcgccaccg gcgacgagat cggcaacttc gacttcaccg
     1261 tcgctatcga caaggccgag ctgccgcccg acggcctcgc cgcgatcgcc acgccgatgg
     1321 cccagctcga ccgccgcgag ctccccgagc gcgtcgagct ctcgacccac aagcagcgct
     1381 ttcgccccgt ctatgacctc ggcgtcgaca tcccgctgac gaagaccgac cagcgccagc
     1441 acagcgagac ccacctcgac cgcgacggcc aggcctactt catgctcgcg ctcaagccgg
     1501 acctcaagct cccggccagc gaccgcccga cgcactacgc gttcgtgctc gaccgcagcc
     1561 acagcatgac ccccgagctg tggaccgtcg cgcgcagcat ccttcaagcg accacgaccg
     1621 cgctcggccc cgacgaccgc atgacggtgc tcgcctgcga cgccgcctgt gacgcggctc
     1681 ctggtggcct cgccccggcc gcgcaggcga tgtccggcgt cgacaccttc ctcgaccagc
     1741 agatcctcgc cggcgccagc gacctcggcg gcatgatgcg cagcgccgcc gaggccctgc
     1801 acgggaccga cgccggcgcg actgcaccgc aggggcccga cgagcccgag cgcatcatcg
     1861 tctacctcgg cgacggcaac ccgaccgccg gcgcgctcgc gcccgacgag ctcctgcgcc
     1921 acctcgagaa gccgctcgcc ggcacccgcg tgcaggccgt cgccctcggc gcccgctccg
     1981 acctgctgtt cctcgacgcc ctcacccgcc gcacgggagg cgacctgctg cgcgccgacg
     2041 ccaaggacga cctgcgcggc ctggtccgcg agctgcggct gcgcgccgag gtcccggtcg
     2101 cgcgcgacgt gcagctgacg ctcccgcgcg gcatggagta cgtgcacccg cagaagttga
     2161 ccgcgctgcg cccgggcgac accgtgctgc tggtcggcaa gctgagcgcg gcggtgcacg
     2221 gcgcgctcga ggtgcaggcc accggtcctt cgggacaggt cgtaaaggac agcttccagg
     2281 tcgacctcga ggcgacgccg agcgccgagg gggcgcgcca cactcacttg ccgcgcacct
     2341 gggcgcgctt cgagatcgac gacctcaccg cgacgaaggg ccacgccgcg cgcgccgaga
     2401 tcatcgacct ctcgcagcag tacaacgtgc tgtcgcgtta caccgcgctg ctcgtgctcg
     2461 agaacgacgc gatgttccgc gagttcaacg tcgtgcggca ggccggcaag accaccggct
     2521 ggaacggcca gctgccgacc ggcaccggca ccgcgcccgg ccagatcgcc agcaaggacc
     2581 ccgccccggc cgccggtgcg cccgcgcccg tcgctccgcc gccgccggtc gccagcccac
     2641 cgagccccga gccggcggac gccggccgcg ccaacgaagc cccgcgtgcc ccggccccga
     2701 aggaggccaa gggtgaagcc gccgccgacg ataatcgccg ggccgaccgt gacgtcgaat
     2761 tgcaggaggt ccaggacccc gcgagccgcg agcagccgcc cgaggaggag ccgccggtcg
     2821 agaagctcga cggcctcgac gacgcgctcg ccagctcgcc cgagcgcgac gcgcccgccg
     2881 acagcaaggc gccgagcgcc aagccggccc cctcgccggc gaaggccaag cgcagcgagg
     2941 ccgccgaccc gatgagcgac agccccggca gcagcgtcgg tggttacggc agcggcggcc
     3001 tcgcgtcccc cggtcgccgc gcgcccgcgc agtggcggat caacgccgcc ggtccgcccg
     3061 cgcccgacgt gctcgccaag ctcgagacga tgcgccaggc ggtcctgcgc gacccgacca
     3121 gccgccccga ccaccgcaag ctcgtgcagt ccgcgatccg tgccggcgcg cccgaggcct
     3181 tcacctacgc gaccgcgtgg tcgcaggccg accccgacca cgcgcccgcg ttgctggccg
     3241 tcgccgacct gctgtccgcc cgcagcgacc ccgcggcgat gcgcgcctac ggctccgccg
     3301 tcgaggtcaa ccccttcaac ctcaagctcc agacccgcct cgccgacgcc tacgccggca
     3361 agggcgacat cacccgcagc tgcgcgcacc gccgcgccgt cgtcagcatc gagccggcaa
     3421 aggccgacaa ccacctgcag ctcgcccgct gcctggctac cttgggccgc caggacctcg
     3481 tgctcgacgc cgtcggcgac ggcctcggcc gcgcgaaggg caacctcgcc gagctgcgcg
     3541 cctcgcagtc cggcctgcgc ccggcccggc cgcgcccggt caccggcaac ctcaaggcga
     3601 cgttgacctg gaacggcgcc ggcgacctcg acgtcgcctt catcgacaac cgcggccggc
     3661 gcctgtcggc cctgcgtccg gagggcgtca cggtcgagca gctcggcaac ggcgagaccg
     3721 ccggtctggc gagcgtctcg ggcacggtca gcgtcgaggt cacgcgcttc agcggtcagg
     3781 gcccggtctc gggcgagctc aaggtgcgca cgccggagac cacccgcgtg ttccccttca
     3841 cgatcgacca gggcaccctg cggctggcca acgtgacctt cacggattat taccgcggtg
     3901 gatggtgatg accggacacg ccctcaggat gtctcttcgg acaggtgtgc tcgccgcgct
     3961 actgcccgcg gtgttcgccg cggtgtttgg cgcgtgcgcg agcgcgccgg cgaccccgac
     4021 cacgggtgtg gtgatcgacc ccgaggcgcc gcaggtcacc ccgcacggtg acctcgggat
     4081 cttgcgccac cgcgccgcga tcgggctgac cgccgacgtg cgcgccgagc tcgccccgcg
     4141 cctcgccgcc gtggcggacc ggcccggacc cgaccccggt cgcgacgccc tgcgcgccct
     4201 ggcgatcgag ctcgcgctga tccagggcga cgccgacgcc gcgcgcgccg agctcgaccg
     4261 cctcgaccgc acgatcgccg gccagggcga gcgcgccagc gccgagctgc gcgccctcgc
     4321 ggcgaccctg cgcggcgcct ggctgttcga cgcccaccgc tacgccgacg cccgcagcag
     4381 ccacctccgg gccctcgtcg cgctcgacca acaaccgccc gacccgcagg gagcggccca
     4441 gaagggcgcc gcgctgcggg ccctcgcccg cgaccagctc gcgctcggcg aggccgaggc
     4501 cgccgtcaac actctcggca aggcgatcga gctccaccgc gacagccccg aggcgcacat
     4561 cgagctccac gaggaccttc tgctcgccgt cgacgtcatg atcgccgtcc agcagcccga
     4621 cgaggccgtc atcgtctcga gcgacgccta caaccaggcg ctgtcgaagt tcggctcgga
     4681 caccttgccg cacgccgagg ccctgctgtt ggtcggcgcg gccaccctcg ccaagggtga
     4741 ccgcagcgcc gcggcgagcg tgatctccga cgcccgggcg atcctcgaca agctgcagtc
     4801 cgagcgcagc gacccccgct tcccgatctc ggcccgcgcc ctgcgtcgca gcgacgacct
     4861 catgcaggcg ctcgcggcct caccgaccga gaagacgacc tctatctgac ctgtcctttg
     4921 ggccctgtcc tcgcggacac ggcggccggg cgtgcaacca ggttcgcggc gttcgggccc
     4981 gtaaacgggt gtttggccgc ccgtacaaga aattgcgtcc cgggctcgcg ggcgcgacga
     5041 cccgtgatcg acatcacgag tcggctcgct gcgagcggtc aagcgggcga tgggagggcg
     5101 acagtgagaa gcggacgcag cgcacctggt ccgcttactt gagccccggc gcgggaccgg
     5161 ggtaccgtgc cacctcgcca tggctggaac atcctccaac tcgaccgtcc acgcgcccga
     5221 acacgagcgg ctccccagcg cgccggcggg ccgcgggctc ctgggtttcc tccgcgactt
     5281 ccacaacaag acgatcctgt ttcgcgtcgg cgactggatc ttcgtcacct acgggctgat
     5341 cgccggcatc gcgttcttct tcgggttctc gacgaccgtc tggtacctgg cgatgatcgg
     5401 ccaggacccg gccgtgaccg cggccgtgtg cctgttcttc gtcatcccgt cggtgctgat
     5461 ggtgtcgcgc gtgacgagca tcctgctcga gtggcgcgag ctgttccggc gtccgctgca
     5521 gaccctgctc aagcccggct acatgctgca cggcgggatc ttcggcggcg tgctggcgat
     5581 ggccggctac gcgatggccg gcggcccgag cctgccgcag ctgctcgacg gcctcggctt
     5641 cgccatgccg ctcggcgagg cgatctgtcg cctcggctgc ttcgtgtacg gctgctgttg
     5701 gggcaagccg accaacagcc gcttcggggt ccgctacacc agcaagcact ccaaggtgat
     5761 ccgcttccag cccgagctgc agggcgtcaa gatccacccg acgcagatct acgcgctgac
     5821 ggcccacctg atccagttca cgatcttcta cgcgctgctg ccctacaagg ccttcgacgg
     5881 catgttcgcg gcgctgtacc tgatcaccca cccgatgatc cgcttcgtcc tcgagacctt
     5941 ccgtcaggac gaccggggta agctcggcaa gctgacccac agcaacatct acagcctgat
     6001 gatgatgggc atcggcctgc tcgtgctcgc gtacggcatg tcgagcgcgg gcaccaacct
     6061 cccgatcgac gtccacttcc gttacatcca cacgatcgcc gacggcgagg cgctcgcgta
     6121 ctgcacgctg accttcttcg tcgcggccgc cgccttcggc gtccactacc gcaacgtcgg
     6181 ctcgtggctg tccaagccgt cgggcggcgt cgcggcgcag gtcgacgagc tcagcctcgg
     6241 cgcctcggcc cgcgacgacg gcctgcacga cgacaattgc gagtcctgcg accaccacga
     6301 gcgttgagcc ttgagcgaca tcacccccga actccatgac ctcgccgccg aggtcgcccg
     6361 ccggcgcacc tttgcgatca tcagccaccc cgacgccggc aagacgacgc tgaccgagaa
     6421 gctgctgctc tactcgggcg cgatccagct ggccggcgcc gtgcgggcca agaaggccgg
     6481 tcgcgcgacc acgagcgact ggatggagat ggagcgcgag cgcggcatct ccgtcaccac
     6541 atcggtgatg agcttcgagt acccgcaccc ggagctgccc gccgacgcgc cgcccgtgca
     6601 ggtcaacctg ctcgatacgc ccggccacgc cgacttcggc gaggacacct atcgcgtgct
     6661 cacggccgtc gacgccgccc tgatggtgat cgacggcgcc aagggcgtcg aggagcgcac
     6721 cgaaaagctg atcgagatct gtcgcctgcg cgacaccccg gtcgtcacgt ttatcaacaa
     6781 gtacgaccgc gagtgcctcg acccgctcga gctgatgggc gacgtcgaga agaagctcgg
     6841 catgacctgc gtgccgctga cctggccggt cggcatgggc aagcgcttca agggcgtcta
     6901 cgacctccag cgccaccgca tgcacatgtt cgcggcgcag gggcatcata aaattcagga
     6961 gggcattttg gtggaagggc tcgacgaccc ccggctcgac gagctgctcg gcgaccaggt
     7021 cgacgagctg cgcatggccg ccgagctgct cggcggcgcc agccagccgc tcgaccgcgc
     7081 ggcgttcctc gccggcaagc agaccccgct gctgttcggc tcggcgatga acaacttcgg
     7141 cgtgcgcgaa ttgttggagg cctacatccg cctggccccc ggcccgctgt cgcgccaggc
     7201 gatcaccggc agcgccgtga cggccccacc cgcccccggc gaggccgtac ccgtgcgcac
     7261 ggtgctcccg accgaggagg cgttcaccgg cttcgtcttc aaaattcagg cgaacatgga
     7321 cccgcagcac cgcgaccgca tggccttcat gcgcgtgtgc tcgggccgct tcgagcgcgg
     7381 catgcgagcg ttccactgcc gcctcggccg cgaggtcggc ctcggcaacg cgacgacctt
     7441 cctcgcccgc gaccgcgaga tcgtcgagac cgcgtacgcc ggcgacatca tcggcctccc
     7501 gaaccacggc acgatcaaga tcggcgacgc gttcacggac ggcgagagcc tgcgcttcac
     7561 cggcatcccc agcttcgccc cggagctctt tcggagggtt ctgctcaaga gcccgctcaa
     7621 ggccaagagc ctcgccaagg gcctgaccca gctcgccgag gaaggcgcga tccaagtttt
     7681 taagcccttc atcggcagcc agttcatcgt cggcgcggtc ggccagctcc agctcgaggt
     7741 gatgaagcac cggttaaaaa gcgagtacga cgtcgacgcc gactacgagg gcgtcgagta
     7801 ctcgacggcc cgctggatca cgccgaagac cgagggcaag tcgaagatcg acgtccagaa
     7861 gctgatggag aacttccaga ccaggaacca gcagaacctg gcgaccgaca gccacggcga
     7921 cctcgcctac ctggcgccga accggtggaa cctgcaaaag gtcgaggagc ggttcccgga
     7981 catccgcttc gcggcgacgc gcgagcacac ctgagtcgtc agcactgctc gctggcgtag
     8041 tcggtgccgg cgatgcaggt ctcgatcggg gcggtgaagg gggcggcgcg caggcactcc
     8101 aggcggcccc gccacccggc ctcggccagg cagccctcga agtacgcggg cgcgggcaga
     8161 gactcgcgct gcacggcgtg ggtgtcccag cacaggtcat agcggtag
//
LOCUS       DASWNJ010000013         1818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_636253, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000013 DASWNJ010000000
VERSION     DASWNJ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636253"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VGB85_00295"
     CDS             complement(<1..315)
                     /locus_tag="VGB85_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132472.1"
                     /translation="MLVLLLLQLGMGWPGAGERGIIAEEVQPYLRHYPVVLGDDPRTD
                     GPRPWPPNDDDAVRAAVTAGEPVAPRWVGTGQWPQLAWQGRERIWPLFVRGHQTALGS
                     WWG"
     gene            433..1299
                     /locus_tag="VGB85_00300"
     CDS             433..1299
                     /locus_tag="VGB85_00300"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEY0132473.1"
                     /translation="MKSRSPRGRLASSFRIALASLRTRQWLHFCVLPLAGLGGEGTYA
                     LFADPAELSRALAGAVIAAGCLGCAYGINAVAERRSDRSARKKPLVAAPGLAAPATAC
                     AIVAGALALGLALIVGPWALLACGLSLLCGVAYSVGLAAKQVPVLGLLLNTGIFAPLL
                     AVLLRPGAVPPSWSHELAIFTLLLIQNQLIHELADRDEDRAAGARTTAQLLGPRGAVR
                     VATLAGLAIPPVALGLAPTPGQWLLGSALAAVATVITADAGRDPARARVVHRRTACAG
                     GALLWLCARLAE"
     gene            complement(1303..>1818)
                     /locus_tag="VGB85_00305"
     CDS             complement(1303..>1818)
                     /locus_tag="VGB85_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0132474.1"
                     /translation="RRARVTRGCLEVIAAADRPWPTLLLTRSASILEDRERIAGLARA
                     WVGVSLPTVDEAVRRHFEPRAATVRERLEVLRTFRALGVRTLAVVQPLLPGSLAAHAD
                     ALAACVDSVSIDVLRGVEGAGELFDDPRHAGSASDEWQLARAHALVELLAARGVPVWR
                     GELPPELAAPP"
BASE COUNT          210 a          715 c          658 g          235 t
ORIGIN      
        1 gccccaccag ctgccgagcg ccgtctggtg cccccgcacg aacagcggcc agatccgttc
       61 gcggccttgc cacgcgagct gtggccactg ccccgtgccg acccagcgcg gcgccacggg
      121 ttcaccggcc gtcaccgccg cgcgcacagc atcgtcgtcg ttgggcggcc acgggcgcgg
      181 cccgtcggtc cggggatcgt cgccgagcac cacgggatag tggcgcaggt acggctggac
      241 ctcctccgcg atgatccccc gctcgccggc gccgggccac cccatgccca gttgcagcag
      301 caacaagacg agcaccgata cggataccgg gacccgggcg gagcgaatcg tggggtgggt
      361 ggcagtcatc ggcgccgcga gagattgtaa gctgctcgcc cggagccggc gacttcgata
      421 cactcgccgc gcgtgaagtc ccgctcaccg cgtggtcgcc tggcgagctc cttcaggatt
      481 gcgctcgcgt cgcttcgcac gcgccaatgg ctgcacttct gcgtgctgcc gctggcgggc
      541 ctcggtggcg aggggacgta cgcgctgttc gccgaccccg ccgagctctc gcgtgcgctc
      601 gctggcgccg tgatcgcggc cggctgcctc ggctgcgcgt atggcatcaa cgctgtcgcc
      661 gagcgccgct cggatcgctc ggcccgcaag aaaccgctgg tcgccgcgcc cgggctcgcc
      721 gcgccggcga ctgcgtgtgc gatcgtcgcg ggtgcgctcg cgctgggcct cgcgctgatc
      781 gtcggcccgt gggcgctgct cgcctgcggg ctctcgctgc tgtgcggcgt cgcctacagc
      841 gtcggcctcg cggccaagca ggtgccggtg ctcgggctgc tgctcaacac cgggatcttc
      901 gccccgctgc tggcggtgct cctgcggccg ggcgcggtcc ccccgagctg gtcgcacgag
      961 ctggcgatct tcaccttgct gctgatccag aaccagctga tccacgagct cgccgatcgc
     1021 gacgaggacc gcgcggccgg ggcccgaacc accgcgcagc tgctcggccc ccgcggcgcc
     1081 gtgcgggtcg ccacgctcgc tggcctcgcg atcccgccgg tcgccctcgg gctcgcgccg
     1141 acccccggcc aatggctgct cggctcggcg ctcgccgccg tcgccaccgt gatcaccgcg
     1201 gacgccgggc gcgacccggc ccgcgcgcgc gtcgtgcatc gcaggaccgc gtgcgccggt
     1261 ggtgcgctgc tgtggctgtg cgcgcggctg gccgaatagg agtcaaggcg gcgccgcgag
     1321 ctcgggcggc agctcgccgc gccacaccgg cacgccacgc gccgcgagca gctcgaccag
     1381 cgcgtgcgcc cgcgcgagct gccactcgtc gctcgcgctg ccggcgtggc gcggatcgtc
     1441 gaacagctcg ccggccccct cgaccccgcg cagcacgtcg atgctgacgg agtcgacgca
     1501 ggccgcgagc gcgtcggcgt gggccgcgag cgagccgggc agcagcggct gcacgaccgc
     1561 gagcgtgcgc accccgaggg cccgaaacgt gcgcaggacc tcgaggcgct cgcgcacggt
     1621 cgcggcgcgc ggctcgaagt gccgccgcac cgcctcgtcg acggtcggca gcgacacccc
     1681 gacccacgcg cgggccaggc cggcgatgcg ctcgcggtcc tcgaggatcg acgccgagcg
     1741 cgtcagcaac agcgtcggcc acggccgatc ggccgcggcg atgacctcga ggcacccgcg
     1801 ggtgaccctc gcccggcg
//
LOCUS       DASWNJ010000014         9546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_106384, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000014 DASWNJ010000000
VERSION     DASWNJ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 9546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9546
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106384"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VGB85_00310"
     CDS             <1..502
                     /locus_tag="VGB85_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY0132475.1"
                     /translation="HVGSFSKVLTPGLRVGWLIAPRVLIEQLLLLKGAADLQTGSLSQ
                     IVLSTYLEHHDLDDHLERCMHHYRARRDATLAALERELSGALRWWRPRSGFSIWVELP
                     DGRPAEQLLRAAVEHGVAFEPGAPCFPCAPAERPQQFLRLCFSNLSPEQIDAGIARLA
                     AALQDG"
     gene            524..1180
                     /locus_tag="VGB85_00315"
     CDS             524..1180
                     /locus_tag="VGB85_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132476.1"
                     /translation="MADDPPGWSWHALADALADGLTRAAAEIELEQAVRGLDACRELE
                     LHPMLHAALRDAGYGVHPEQRFPRERGKRRRSEGSRCDIVLTPEGRPLANEEVQLGLF
                     APKQAVPLSEAMWIEVKAVAQFREMAPNRAYAQALQKPVWKDVEKLAADPEIAHAVVL
                     LVIFTADAVVAEHDLEVWALRASQRGLPLWPRIQRSLAIGDRIGNRLCTLALFPIERR
                     "
     gene            complement(1199..2680)
                     /locus_tag="VGB85_00320"
     CDS             complement(1199..2680)
                     /locus_tag="VGB85_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132477.1"
                     /translation="MDLASSLLIGLGGCGDDVADSDSAGSTGKLTTTTPLTTGETATG
                     SSGNLPTTGDGSAEGTVSDSGTSSGSTAPTTDVTTEGTSTTDASTTTGMTTTGVVPDT
                     TTGDTTTGASGSTSDASTTTDTTTGGNNGCEVKCGNSDWSYVWIANSGENTVSKLNTR
                     DMTEEGRYYTRPDKQGSPSRTSVSIDGKAVVVANRHGGLTKIWARKEFCTDKNGNGQI
                     ETSTGKNNVLPFELDECIAWHTPFPGATTQRPVAWTSGVYNEQTCEYEDQQIWAAASF
                     GADTGNPCQGAQGITVFRVNGDTGMVDDTIPLPQVSCGLLGAYGAAVDTKNNAWFFVF
                     GGSIIFKVDHETLAVEVVNKGFYGITVDSKDRVWLDDGSRYDPVTKQWASQIGDLQPN
                     GGSGVAIDLQGRVWHATDGGLGWLDAETMVVGGKVNIPNNGFSRGIGVDVDGYIWSVP
                     LGGTMAHRIHPETFEIATYTGLNQPYTYSDMAGGQINNVVCPQ"
     gene            complement(2883..4589)
                     /locus_tag="VGB85_00325"
     CDS             complement(2883..4589)
                     /locus_tag="VGB85_00325"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY0132478.1"
                     /translation="MTIDRRKLRWNGWGLAHATFDMGHREPAVWDYIARALGREQLAR
                     TPAVALPDIQLPPTRLDDTTLAALQGIVGGSNVKTDRYERAFHSRGKSYLDLIALRAG
                     ELGEHVPDAVVYPGNADEVQSVLDLAVSRELAVVPFGGGSSVVGGVTAQRGRRAAVLT
                     VDMTRMHRLLSIDEVSHAATLEAGVYGPELERQLQLRGYTLGHYPQSFEFSTLGGWIA
                     ARGAGQQSNRYGKAEKWLLGARLATPRGPWQTEQFPASAAGPNLNQLVLGSEGTLGII
                     TEATVRIHRVPAAQDYRAYLFRSFAEGAAAIREIVQRQIPVAMLRLSDAHETHFLQSF
                     SAAQHPPGMATRAGAQVVTWRGFGEARCLLLVGLEGERETVLESQVQTRSVCTRHGGM
                     SIGASPGRKWYARRFAMPYLRDPLLDRGIAVETLETATRWSNIETLHDEIDAAIRKAL
                     AANAVDERARAIVMCHISHAYEDGASLYFTMVFPQKLGDDSQSAASAARGQWLAVKAA
                     ATEAMIAGGGTITHHHGVGTDHAPWLPREKGPIGLSALAAIKEDLDPAGAMNPGKVLD
                     MG"
     gene            complement(4594..5538)
                     /locus_tag="VGB85_00330"
     CDS             complement(4594..5538)
                     /locus_tag="VGB85_00330"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY0132479.1"
                     /translation="MRVRAIINPNSSHGRTRAAWPKLARALEAELGPVEAVFTDAPLS
                     ATLRTREALQEDVDLVIAVGGDGTVNEVVNGFFAPPSAAGDLPLRPETELAILMSGTG
                     GDFRKTFELGADLPAQVQRIARGNVRAIDIGRIDYTDHEGRPAARYFNNIASFGLSGL
                     VSDRVNRARLSKRVSGRFAFFVASVQSVLGYKMPRARIVVDDQPPLEIGVNTVAVCNG
                     RYFGGGMHMAPMARPDDGLFDLVVMHDMTTRDLLRDPNAIYRGTHIENPKVLTLRGRR
                     VVATPLPGPHALLDVDGETPGRLPATFTLLPAVLKFRC"
     gene            complement(5551..7167)
                     /locus_tag="VGB85_00335"
     CDS             complement(5551..7167)
                     /locus_tag="VGB85_00335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEY0132480.1"
                     /translation="MRPDLARASAQAHDLIVVGGGVTGACIARDAAMRGLSVVLLEKQ
                     DFSHATSAASSKLIHGGLRYLKNLEVGLIRESLRERRMWLRTAPHLVYPLAMLLPVYR
                     GHPESLLLLRAGLTVYDLLAFDRRWLADPDQRLPGHRVLRPAAALAQQPDLPSEGLVG
                     ALTYYDCQMFSPERLALECLISAASAGAQLFNYTEVVELLRTDHTVTGVRARDAAGTV
                     VDLHARLVINAAGPWADLVLGDTESAQKLMRSKGVHIITRSLTVGHALAIASDGGHFF
                     VLPWRGHSIIGTTDEAYEGAPDDFRVTEGDITGLLATVNKHLPNAQLAREDVLHFYGG
                     LRPLVDDGASTYDASRRSEVCDHEKLGGPAGLLSAVGGKWTTSRHLAEQVVDLALRKL
                     GRKRVACSTGDTPLPGGAIGNFRAFVGAMIRRHPGHDVALIEHLARNYGGRMGEVLRL
                     ADADVSLARPLADGLPEIGAQVVHAVRAEMAVSLGDVVLRRTGLGTLGDPGEAALMAA
                     ARFMGGLLGWDRAERLRQVERVLDHYVAAT"
     gene            7312..8292
                     /locus_tag="VGB85_00340"
     CDS             7312..8292
                     /locus_tag="VGB85_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HEY0132481.1"
                     /translation="MTNDRPVSSFIRRHYRHFNAAALVDAAEGYNRHLASGGKMLVTI
                     AGAMSTAELGISLAEMIRQGKIHAISCTGANLEEDVFNLVAHDHYERVPNYRQLTPQD
                     EQALLERHMNRVTDTCIPEMEAMRRIEDAVLAEWVRADQAGERMFPHEFFYKILLSGV
                     LEGSYQIDPKDSWLLAAAQANLPMIVPGWEDSTLGNMFAGHVIAGDVKNVHTVRTGIE
                     YMMVWADWYTKISKQGSLGFFQIGGGIAGDFPICVVPMLHQDLRRTDVPLWGYFAQVS
                     DSTTSYGSYSGAVPGEKITWGKLGVDTPGYIIESDATIVVPLIFAYVLGW"
     gene            8298..9341
                     /locus_tag="VGB85_00345"
     CDS             8298..9341
                     /locus_tag="VGB85_00345"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEY0132482.1"
                     /translation="MHPRAAFENHELTILTDSAAALGYRMPAEWTRLDAVWLSRPHNP
                     TTWPGCLAEAQAQHASFAEVLRRFVAVQVVGTDLPLTTDDAWIRDYGPIFVKNAKGEL
                     ACHDFHFNNWGGKYGDCGNDDIVPQHIARLLGVPVWIHDFVLEGGSIEVNGVGTVMTS
                     ESCLLGPGRNTGMTRAQIEAALHSYLGTSHVIWLPAGIEGDDTDGHIDDLARFVDAST
                     VAAVRTPVGHPDHAVLERNWELLGEARDQHGKRIERVALPVPEPRNWYFPPEDDWAGG
                     ERLVPASYANFLIANDGVLVPTFAQGSDDVALRLFEQLFPGKQIVGVPAQYLVVGLGS
                     LHCLSQQQPAAGG"
     gene            complement(9351..>9546)
                     /locus_tag="VGB85_00350"
     CDS             complement(9351..>9546)
                     /locus_tag="VGB85_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132483.1"
                     /translation="EVLAPLLLGVRRLRPLGILMGVGMHVFIAVNMHLLIYFSLQILC
                     FYVLFLDADLLSRLRAKLPR"
BASE COUNT         1398 a         3099 c         3561 g         1488 t
ORIGIN      
        1 ccatgtcggc agtttttcaa aagtgctgac gccgggattg cgggtcgggt ggttgatcgc
       61 gccgcgggtg ctcattgaac agctgctcct gctcaagggc gcggcggacc tgcagacggg
      121 gtcgctcagc cagatcgtgc tgtcgaccta cctcgagcat cacgacctcg acgaccacct
      181 cgagcggtgc atgcatcact accgggccag gcgcgacgcg acgctcgcgg ccctcgagcg
      241 cgagctgtcc ggcgcgctgc gctggtggcg accgcggtcg ggattctcga tatgggtcga
      301 gttgcccgat gggcggcccg cggaacagtt gctgcgcgcg gcggtcgagc acggcgtcgc
      361 cttcgagccc ggtgcaccct gctttccgtg cgcgcctgcg gagcgcccgc agcagttcct
      421 gcgcctgtgt ttttcaaatc tctctcccga gcagatcgac gcgggcatcg ctcgcctcgc
      481 ggctgcgctg caggacggct gagtcgcgct atcctccggg cggatggcgg acgacccccc
      541 gggctggtcc tggcacgcgc tggccgatgc gctcgcggac ggcctgacgc gcgcggccgc
      601 ggagatcgag ctcgagcagg cggtgcgcgg gctcgatgcg tgccgcgagc tcgagctgca
      661 tccgatgttg cacgcggcgc tgcgcgacgc gggctacggc gtgcacccgg agcagcggtt
      721 cccgcgtgag cgcggcaaac ggcgtcgctc cgagggttcg cgctgcgaca tcgtgttgac
      781 gccggagggt cggccgctgg cgaacgagga ggtgcagctc gggctgttcg cgccgaagca
      841 ggcggtgccg ctgtcggagg cgatgtggat cgaggtcaag gcggtcgcgc agttccgcga
      901 gatggcgccc aaccgggcct acgcgcaggc gctgcagaag ccggtgtgga aggacgtcga
      961 gaagctcgcc gcggacccgg agatcgccca cgcggtcgtg ctgctggtga tcttcacggc
     1021 cgacgccgtg gtcgccgagc acgacctcga ggtgtgggcc ctgcgggcga gccagcgcgg
     1081 cctgccgctg tggccgcgga tccagcggtc actggcgatc ggcgaccgga tcgggaaccg
     1141 cctgtgcacg ctggcgctgt tcccgatcga acgccgatga tgacgatgac ggcgatgact
     1201 actgcgggca gacgacgttg ttgatctgtc cgccggccat gtccgagtac gtgtagggct
     1261 ggttgaggcc ggtgtaggtc gcgatctcga aggtctcggg gtggatgcgg tgggccatcg
     1321 tgccgccgag cgggaccgac cagatgtagc cgtcgacgtc gacgccgatg ccgcgcgaaa
     1381 acccgttgtt cgggatgttg accttgccgc cgacgaccat cgtctcggcg tcgagccagc
     1441 cgaggccgcc gtcggtcgcg tgccagacgc ggccctggag gtcgatcgcg accccgctgc
     1501 cgccgttggg ctggaggtcg ccgatctggc tggcccactg cttggtcacc gggtcgtagc
     1561 gcgagccgtc atcgagccac acgcgatcct tgctgtcgac ggtgatcccg taaaagccct
     1621 tgttgacgac ctcgacggcg agggtctcgt ggtcgacctt gaagatgatg ctgccgccga
     1681 acacgaagaa ccacgcgttg ttcttggtgt cgaccgcggc gccgtaggcc ccgagcaggc
     1741 cgcaggacac ctgcggcagc gggatggtgt catcgaccat gccggtgtcg ccgttgacgc
     1801 ggaacacggt gatgccctgc gcgccctgac acgggttgcc cgtgtcggcg ccgaagctgg
     1861 cagcggccca gatctgctgg tcctcgtact cgcaggtctg ctcgttgtag acgccggacg
     1921 tccaggcgac ggggcgctgc gtggtcgcgc cggggaaggg tgtgtgccag gcgatgcact
     1981 cgtcgagctc gaacgggagc acgttgttct tgccggtcga ggtctcgatc tggccgttgc
     2041 cgttcttgtc ggtgcaaaac tccttgcgtg cccagatctt ggtcaggcca ccgtgacggt
     2101 tggcgacgac gacggccttg ccgtcgatgc tgaccgaggt gcgcgacggg ctgccctgct
     2161 tgtccgggcg cgtgtagtag cggccctcct cggtcatgtc gcgcgtgttg agcttgctga
     2221 ccgtgttctc gccgctgttg gcgatccaca cgtagctcca gtcgctgttg ccacacttga
     2281 cctcacaacc attgttgccg ccggtcgtcg tgtcggtggt ggtgctcgcg tcgctcgtgc
     2341 tgccgctcgc gccggtcgtc gtgtcgccgg tcgtcgtgtc cggcacgaca ccggtggtcg
     2401 tcatgccggt cgtggtgctc gcgtcggtgg tgctcgtgcc ctcggtcgtc acgtcggtgg
     2461 tcggcgcggt cgagccggag ctggtgccgc tgtccgagac ggtgccctcg gccgagccgt
     2521 cgccggtggt cggcaggttg ccggaggagc ccgtcgcagt ctcgccagtg gtcagcggcg
     2581 tggtcgtggt cagctttccg gtgctgcccg cgctgtcgct gtcggcgacg tcgtcgccgc
     2641 agccgccgag gccgatcaag agggaagacg cgagatccac ggagcagcgc tgtaacgagc
     2701 catgggatcc accctaactg caccgagggg aacgccgcaa gcgaggacat cgcgggcgga
     2761 tccatccacg actcacggac cggcccgtca gccttgcggg ttgcaggtca cgttgttgat
     2821 ctggctgccg cccatgtccg agaaggggtt ggctcgccac cggtagcggc ggcgcgcagg
     2881 cgtcagccca tgtcgaggac cttgcccggg ttcatcgcgc cggcggggtc gaggtcctcc
     2941 ttgatcgccg cgagcgcgga caggccgatc gggcccttct cgcgcgggag ccacggggcg
     3001 tggtcggtgc cgacgccgtg gtggtgcgtg atggtgccgc caccggcgat catcgcctcg
     3061 gtcgcggcgg ccttgacggc gagccactgg ccgcgcgcgg ccgaggcggc cgactgcgag
     3121 tcgtcgccga gcttttgcgg gaacaccatc gtgaagtaca ggctggcccc gtcctcgtag
     3181 gcgtgcgaga tgtgacacat gacgatcgcg cgggcgcgct cgtcgacggc gttggcggcg
     3241 agggccttgc ggatcgcagc gtcgatctcg tcgtgcaggg tctcgatgtt ggaccagcgg
     3301 gtcgcggtct cgagggtctc gacggcgatg ccgcggtcga gcagcgggtc gcgcaggtag
     3361 ggcatggcga agcgccgcgc gtaccacttg cggcccgggc tcgcgccgat cgacatgccg
     3421 ccgtgacgcg tgcacacgct gcgggtctgg acctgcgact cgagcacggt ctctcgctcg
     3481 ccctcgaggc cgacgagcaa caggcagcgg gcctcgccga agccgcgcca cgtgacgacc
     3541 tgcgcccccg cgcgcgtggc catgccgggc gggtgctggg cggcggagaa gctttgtaaa
     3601 aagtgggtct cgtgggcgtc ggagaggcgc agcatcgcca cggggatctg gcgttgcacg
     3661 atctcccgga tcgcagcggc cccctcggcg aaggagcgaa acaggtaagc gcggtagtcc
     3721 tgcgccgccg gcacgcggtg gatgcggacc gtcgcctcgg tgatgatgcc gagcgtgccc
     3781 tcgctgccga gcacgagctg gttgaggttc ggaccggcgg cggaggccgg gaactgctcg
     3841 gtttgccagg gcccgcgcgg cgtcgccagg cgcgcgccga gcagccactt ctcggccttg
     3901 ccgtagcgat tgctctgctg accggctcca cgcgcggcga tccatccgcc gagggtcgag
     3961 aactcgaagg actgcgggta gtgaccgagg gtgtagccgc gcagctgcag ctggcgctcg
     4021 agctccgggc cgtacacgcc ggcctcgagc gtcgccgcgt gcgagacctc gtcgatgctc
     4081 agcaggcgat gcatgcgcgt catgtcgacg gtcaacaccg cggcgcgccg gcctcgctgg
     4141 gccgtgacgc cgccgacgac gctcgagccg ccgccaaagg gcacgaccgc gagctcgcgc
     4201 gagacggcga ggtcgagcac gctctggacc tcgtcggcgt tgccgggata cacgacggcg
     4261 tcgggcacgt gctcgccgag ctcaccggcg cgcagggcga tgaggtcgag gtagctcttg
     4321 ccgcggctgt ggaaggcgcg ctcgtagcgg tcggtcttga cgttggaccc gccgacgatg
     4381 ccttgcaggg cggcgagggt ggtgtcgtcg aggcgcgtcg gtgggagctg gatgtcgggc
     4441 aaggcgacgg cgggtgtgcg cgcgagttgc tcgcggccga gggccctggc gatgtagtcc
     4501 cacaccgcgg gctcgcggtg gcccatgtcg aaggtggcgt gggcgaggcc ccagccgttc
     4561 cagcgcagct ttcgccgatc gatggtcatg tgattagcac ctgaacttga ggacagccgg
     4621 taagagggtg aaggtggcgg ggagtcggcc gggggtctca ccgtcgacgt cgagcagggc
     4681 gtgcggtccg ggcagcgggg tggcgacgac gcgtcggcct cgcagggtca ggaccttggg
     4741 gttctcgatg tgcgtgccgc ggtagatcgc gttggggtcg cgcagcaggt cgcgggtggt
     4801 catgtcgtgc atcacgacga ggtcgaacag gccgtcgtcg gggcgggcca tcggcgccat
     4861 gtgcatgccg ccgccgaagt agcggccgtt gcacaccgcg acggtgttga cgccgatctc
     4921 tagcggcggc tggtcgtcga cgacgatgcg cgcgcgcggc atcttgtagc cgagcacgct
     4981 ctgcaccgag gcgacgaaga acgcgaagcg gccgctgacg cgcttgctga gccgggcccg
     5041 gttgacgcgg tccgagacca ggccggacag gccgaagctg gcgatattat taaagtagcg
     5101 ggcggccggg cggccctcgt ggtcggtgta gtcgatgcga ccgatgtcga tcgcgcgcac
     5161 gttgccgcgg gcgatccgct ggacctgcgc gggcaggtcg gcgccgagct cgaaggtctt
     5221 gcgaaagtcg ccgccggtgc ccgacatcag gatcgccagc tcggtctcgg ggcgcagcgg
     5281 gagatctccg gcggcgctcg gcggggcgaa gaagccgttg acgacctcgt tgacggtgcc
     5341 gtcgccgccg accgcgatca cgaggtcgac gtcctcctgc agcgcctcgc gggtgcgcag
     5401 ggtcgccgac agcggggcat cggtgaacac ggcctcgacc gggccgagct cggcctcgag
     5461 ggcgcgcgcg agcttcggcc aggccgcacg cgtgcggcca tgggaggaat tcgggttgat
     5521 gatcgcccgg acgcgcatac ggtcagagga tcatgtcgcc gcgacgtaat ggtcaagcac
     5581 ccgctcgacc tgacgcagcc gctcggcccg gtcccagccg agcagtccgc ccatgaagcg
     5641 ggccgcggcc atcagcgccg cctcgccggg gtccccgagg gtgccgaggc cggtgcgccg
     5701 cagaacaacg tccccgagcg acacggccat ctcggcccgg accgcgtgga cgacctgcgc
     5761 gccgatctcg ggcaggccgt cggcgagcgg acgggcgagc gagacgtcgg cgtcggcgag
     5821 gcgcaggacc tcgcccatgc gaccgccgta gttgcgcgcg aggtgctcga tcagcgcgac
     5881 gtcatgaccg gggtggcggc ggatcatggc gccgacgaac gcgcgaaagt tcccgatcgc
     5941 gccgccgggc agcggggtgt cgccggtgct gcacgcgacg cgcttgcggc cgagcttgcg
     6001 cagcgcgagg tcgacgacct gctcggcgag gtgtcgcgag gtcgtccact tgccaccgac
     6061 cgcggacagc agacccgcgg ggccgccgag cttctcgtga tcgcagacct cggagcggcg
     6121 actcgcgtcg taggtgctcg cgccgtcgtc caccagcggg cgaaggccgc cgtagaagtg
     6181 caggacgtcc tcgcgggcga gctgcgcgtt cggcaggtgc ttgttgacgg tcgcgagcag
     6241 gccggtgatg tcgccctcgg tgacgcgaaa gtcgtcgggc gcgccctcgt aggcctcatc
     6301 ggtggtgccg atgatgctgt gcccgcgcca cggcaggacg aagaagtgcc cgccgtcgct
     6361 ggcgatcgcc agggcgtggc cgacggtgag cgagcgcgtg atgatgtgga cgcccttgga
     6421 gcgcatcagc ttctgcgcgc tctcggtgtc gccgagcacg aggtccgccc acggacccgc
     6481 ggcgttgatc acgagcctgg cgtggaggtc gacgacggtc ccggcggcgt cgcgcgcacg
     6541 cacgccggtg acggtgtggt cggtgcggag cagctcgacg acctcggtgt agttgaacag
     6601 ctgcgcgccc gccgatgcgg cggagatcag gcactcgagc gcgaggcgct cgggcgagaa
     6661 catctggcag tcgtagtagg tcagcgcgcc gacgaggccc tcggacggga ggtcgggctg
     6721 ctgcgcgagg gcggcggcgg gcctcagcac acgatgcccg ggcaggcgtt ggtcggggtc
     6781 ggcgagccag cggcggtcga aggcgaggag gtcgtagacg gtcaggcccg cgcgcagcag
     6841 cagcaggctc tcggggtggc cgcggtacac cggcagcagc atcgccagcg ggtagacgag
     6901 gtgcggggcc gtgcgcagcc acatccggcg ctcgcgcagg gactcgcgga tcaggccgac
     6961 ctcgaggttc ttgaggtagc gcaggccgcc gtggatcagc ttcgaggagg cggcgctggt
     7021 cgcatgagag aagtcctgct tctccagcaa gacgaccgac aggccgcgca tggccgcatc
     7081 gcgggcgatg cacgcgccgg tgactccacc gccgacgacg atgaggtcgt gggcctgtgc
     7141 gctcgcgcgg gccaggtcgg gtcgcatgcc ggggcaaggc tactcgcctc accggcccgc
     7201 gcaatggccg gaggacgcgc agggcggccg aaccgcctgt tcgcggaccg cgcagacccc
     7261 gcaaaaagcc cggcatgggg ccctgggatc gcgtatcctc gcgccccgca catgacgaac
     7321 gatcgtccag tctccagctt tattcgcagg cattaccgcc atttcaacgc ggcggcgttg
     7381 gtcgacgccg ccgagggcta caaccgacac ctcgcgagcg gcggcaagat gctcgtgacg
     7441 atcgccgggg cgatgagcac cgcggagctg ggcatcagcc tggcggagat gatccgccag
     7501 ggcaagatcc acgcgatctc ctgcacgggc gcgaacctcg aggaggacgt gttcaacctc
     7561 gtcgcgcacg accactacga gcgcgtcccg aactaccgcc agctgacgcc gcaagacgag
     7621 caggcgctgc tcgagcgcca catgaatcgc gtcaccgaca cctgcatccc cgagatggag
     7681 gcgatgcggc gcatcgagga cgcggtgctc gccgagtggg tgcgcgccga ccaggccggc
     7741 gagcgcatgt tcccgcacga gttcttttac aagatcctgc tcagcggggt cctcgagggg
     7801 agctatcaga tcgacccgaa ggactcgtgg ctgctcgcgg cggcgcaggc caacttgccg
     7861 atgatcgtgc ccggctggga agactcgacg ctcggcaaca tgttcgccgg ccacgtgatc
     7921 gccggcgacg tcaagaacgt gcacacggtg cgcaccggca tcgagtacat gatggtctgg
     7981 gccgactggt acacgaagat ctcgaagcag ggcagcctcg gcttcttcca gatcggcggc
     8041 ggcatcgcgg gcgacttccc gatctgcgtg gtgccgatgc tccaccagga cctgcgccgc
     8101 accgacgtgc cgctgtgggg ctactttgcc caggtcagcg actcgaccac gagctatggc
     8161 tcgtactcgg gcgcggtccc gggcgagaag atcacgtggg gtaagctcgg cgtcgatacg
     8221 cccggctaca tcatcgagtc cgacgcgacg atcgtcgtgc cgctgatctt tgcttatgtg
     8281 ctcggctggt gatcgccatg cacccgcgcg ccgcttttga gaatcatgag ctgacgatcc
     8341 tgacggactc cgcggcggcg ctcggctacc gcatgcccgc cgagtggacc aggctcgacg
     8401 cggtgtggct cagtcgcccg cacaacccca ccacatggcc cggctgcctc gccgaggccc
     8461 aggcgcagca cgcgagcttc gccgaggtgt tgcgccgctt cgtcgcggtg caggtggtag
     8521 gcaccgacct gccgctgacc accgacgacg cgtggatccg cgactacggg cccatctttg
     8581 taaaaaacgc gaagggcgag ctcgcttgcc acgacttcca ttttaataat tggggcggca
     8641 agtacggcga ttgtggcaac gacgatatcg tgccacaaca catcgcccgc ctgctcggcg
     8701 tgccggtgtg gatccacgac ttcgtgctcg agggtggttc gatcgaggtc aacggcgtcg
     8761 gcacggtgat gaccagcgag tcgtgcctgc tcgggcccgg gcgtaatacc gggatgacgc
     8821 gcgcgcagat cgaagcggcg ctgcactcgt atctcggtac gtcgcacgtc atctggctgc
     8881 cggccggcat cgagggcgac gacaccgatg gacatatcga tgacctcgcg cgcttcgtcg
     8941 atgcgtcgac ggtcgccgcg gtgcgcacgc ccgtggggca ccccgaccac gcggtgctcg
     9001 agcgcaactg ggaactgctc ggcgaggcga gagaccagca cggcaagcgc atcgagaggg
     9061 tcgcgctgcc ggtgcccgag ccgcgcaact ggtacttccc gcccgaggac gactgggccg
     9121 gcggcgagcg cctggtgccc gcgagctacg cgaactttct catcgccaac gacggggtgc
     9181 tcgtgccgac cttcgcccag gggagcgatg acgtggcgct gcgcctgttc gaacagctgt
     9241 ttccgggcaa gcagatcgtc ggggtgccgg cgcagtacct cgtggtcggg ctggggtcgc
     9301 tgcactgctt gagtcagcag cagcctgctg ctggcgggtg aggcgggccg tcagcgcggg
     9361 agcttcgcgc gcaggcgaga cagcaggtcg gcgtcgagga agagcacgta gaagcagagg
     9421 atctgcaggc tgaagtaaat tagaaggtgc atgttgacgg cgatgaagac gtgcatgccg
     9481 acgcccatca ggatgccgag cgggcgcagg cggcggacgc cgagcaggag cggggcgagg
     9541 acctcg
//
LOCUS       DASWNJ010000015         1814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_141503, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000015 DASWNJ010000000
VERSION     DASWNJ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141503"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VGB85_00355"
     CDS             <1..234
                     /locus_tag="VGB85_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132484.1"
                     /translation="SALLGARTGGVLGGRVGFAVLVGARRHHELGMQLTLAFHGFGPG
                     PSAPACDAPGRVLQDVPRARLMVTGALGYAFRF"
     gene            312..1745
                     /locus_tag="VGB85_00360"
     CDS             312..1745
                     /locus_tag="VGB85_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0132485.1"
                     /translation="MTATPGQFYRWLDEVVPRAGSQRSLDSQDTTLSRRGLLIRADKR
                     ARELAGLKIGAGDIVALSMGNVAEFVILLLAVSKLGAIAMPVDPANGDRSLLGAASRL
                     PVRAVLRRPRGLENSPLEYPEGYKLLARRKLSGSLLEVDTLEVPEELRAIELPADTEY
                     ICETAGADGVLHDVLRSGAHLRWIGENAASMLDLAAGTHLACAQAFTSPRFFDPVVLG
                     WLASEARLVMADNPTLESVLPPVGTYDRLVVVDLLYSLQTAARALKAAGAGRELLPVI
                     PQATIATSHGKMLKQIYGEHPRQLLLLEEGGILGVRAMHRGERFEPAPGIELRPGAPH
                     QGGHEVLFRPRLPVVTRPPLPAGQPGGPVEGSPGWYHTGYLGHFNAQGALGEVPGRTD
                     SLVNLEGRRASLRTIEAAMLRHRRITGAEAAVEYDVDGNPFVHLRYRATGLTDLDDLD
                     EFMVDSLPPFMVPRQTTRVDTTPSDPG"
BASE COUNT          253 a          705 c          602 g          254 t
ORIGIN      
        1 agtgcgctgc tgggcgcgcg cacgggcggc gtgctcggtg gacgcgtcgg gttcgctgtg
       61 ttagtggggg cgcgccgtca ccacgagctc ggcatgcaac tgacactcgc cttccatggc
      121 ttcggccccg gccccagcgc ccccgcctgc gacgcgcccg gacgtgtgct gcaagatgtc
      181 ccgagagcca ggctcatggt gaccggcgcg ctcggctacg cgttccgctt ctgaaccgag
      241 gtgcagaagg tgcagaaggt gcagatcatg ccgaagtcga cgcacccgcg actctccagg
      301 gaggtcacgc gatgaccgcc acacccggac aattctaccg ctggctcgac gaggtcgtgc
      361 cccgcgcggg cagccagcga tcgctcgaca gccaggacac cacgctcagc cgtcgcggcc
      421 tgttgatccg cgccgacaag cgggcccgcg agctcgccgg cctcaagatc ggcgccgggg
      481 acatcgtcgc cctgtcgatg ggcaacgtcg ccgagtttgt catcctcctg ctcgcggtga
      541 gcaagctcgg cgcgatcgcc atgccggtcg accccgccaa cggcgaccgc agcctgctcg
      601 gcgccgccag ccgcctgccc gtgcgcgccg tgctgcgccg cccgcgtggc ctcgagaact
      661 cgccgctcga gtaccccgag ggttacaagc tgctcgcgcg gcgcaagctc agtggttcgc
      721 tgctcgaggt cgacaccctc gaggtccccg aggagctgcg ggcgatcgag ctgcccgccg
      781 acaccgagta catctgcgag accgcggggg ccgacggcgt cctgcacgat gtcctgcgca
      841 gcggcgccca cctgcgctgg atcggcgaga acgcggcgag catgctcgac ctcgcggccg
      901 gcacccacct cgcctgcgcc caggcgttca ccagcccacg cttcttcgac cccgtcgtgc
      961 tcggctggct cgcctccgag gcgcgcctgg tgatggccga caacccgacc ctcgagtccg
     1021 tgctgccgcc cgtcggcacc tacgaccgcc tcgtcgtcgt cgacctgctg tactcgttgc
     1081 agaccgccgc gcgcgcgctc aaggccgccg gcgccggtcg cgagctgctc ccggtgatcc
     1141 cccaggcgac gatcgccacc agccacggaa agatgctcaa gcagatctac ggcgagcatc
     1201 cgcgccagct gctgttgctc gaggagggcg ggatcctcgg cgtccgcgcg atgcaccgcg
     1261 gcgagcgctt cgagcccgcg cctggcatcg agctgcgccc cggcgccccg caccagggcg
     1321 gccacgaggt gttgttccgc ccgcgcctgc cggtcgtaac tcgcccgccc ttgcccgcgg
     1381 gccagcccgg cgggccggtc gaggggtcgc ccggctggta tcacaccggg tacctcggcc
     1441 acttcaacgc ccagggcgcg ctcggcgagg tcccgggccg caccgacagc ctcgtcaacc
     1501 tcgagggtcg ccgcgcttcg ctgcgcacga tcgaggccgc catgctgcgc caccgccgga
     1561 tcaccggggc cgaggccgcc gtcgagtacg acgtcgacgg caaccccttc gtgcacctgc
     1621 gctacagggc caccggcctg accgacctcg acgacctcga cgagttcatg gtcgacagcc
     1681 tgccgccttt catggtcccg cggcagacga cgcgcgtcga cacgacgccg tccgatccag
     1741 ggtgaacgac atgtccggcg gccagatcct cagcgtcctc ccgcggtgac gaacaccggc
     1801 gccggtcagc gctg
//
LOCUS       DASWNJ010000016         1841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_71054, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000016 DASWNJ010000000
VERSION     DASWNJ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71054"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..>1841
                     /locus_tag="VGB85_00365"
     CDS             118..>1841
                     /locus_tag="VGB85_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132486.1"
                     /translation="MTQRSRLNYCSLTTLVLITACPGSGDDTGGGGTTTTATNPTTGA
                     STPTTGGPVTEATTGTSAASEPTTTTGGTDATDTASGTTTDTSGTTAMTGMTGTDTGD
                     SALVITPDNVVLELVDGNIVTQDFTATLDGKDVTAEVVWVYDQEAIGAMQTATFVPTG
                     TLAGPGTLTATHPQAVDDTQVTVKIKKTIDPDGLAPGFGDPVGPDPALQVLYPYDNTV
                     FPLKVASPVVQWGGVPDGSQYKLHLQEQFYEYTVYFTANNPARHLINEVEWIAISDSG
                     QGAKSDPLSFELQRKTGDMTFEAVKQTWKIAQARLPGRVYYWELPSQCGNNNGRVLSI
                     KPTEAKAEEFFKPGQCYGCHSVSRDGRTVSVAYDTGVPFPLGTIDVSAEPAVQGPITP
                     NSGLAGTFSAFNHDGTKLLFSNDAVNAAASGLVIADTMTAQVLNPNVMGSGCGEPAWS
                     PDGTLIAATCGYTDFGWTFDAQTAELRIATVNPDGITVSDIKTIVPQMGDVGRPAYPN
                     FSPGNEWIVYGRPTQGSRSTGNGKLYMVGVDGQNNRTLEIASSDNKSFNPTFAPLRAG
                     GYFWVVFM"
BASE COUNT          334 a          662 c          607 g          238 t
ORIGIN      
        1 atgaggttca tcgggggcgc ccggccgcgc gcgagtatac cccgacgtgc gcgcccgtcg
       61 tacgcatggg gtctttctcg cttgacgcgc ccggcgtgcg cttgaacagt ggaccgcatg
      121 acgcagcgat cgcgcttgaa ctattgcagc ttgacgaccc tcgtactgat caccgcctgc
      181 cccggcagtg gcgatgacac cggcggcggc ggcacgacca ccacggcgac caacccgacg
      241 accggcgcat cgacgccgac caccggcggt ccggtgaccg aggcgaccac cggcaccagc
      301 gccgccagcg agccgacgac gacgaccggc ggcacggacg cgacagacac ggccagcggc
      361 acgaccacgg acacgtcggg cacgacggcg atgaccggca tgaccggcac cgacaccggc
      421 gacagcgcgc tggtgatcac gcccgacaac gtcgtgctcg agctcgtcga cggcaacatc
      481 gtcacgcagg acttcacggc gacgctcgac ggcaaggacg tcaccgccga ggtcgtgtgg
      541 gtctacgacc aggaggcgat cggcgcgatg cagaccgcga ccttcgtgcc gaccggcacg
      601 ctcgcgggcc cgggcacgtt gacggcgacg cacccgcagg cggtcgacga cacccaggtg
      661 accgtcaaga tcaagaagac gatcgacccc gacgggctcg cgccgggctt tggcgatccg
      721 gtcggccccg acccggcgct ccaggtcctc tacccctacg acaacacggt gttcccgctc
      781 aaggtcgcgt cgccggtggt ccagtggggc ggcgtgcccg acggcagcca gtacaagctg
      841 cacctgcagg agcagttcta cgagtacacg gtctacttca cggcgaacaa ccccgcgcgc
      901 cacctgatca acgaggtcga gtggatcgcg atctccgact ccgggcaggg cgccaagagc
      961 gacccgctga gcttcgagct gcagcgcaag accggcgaca tgaccttcga ggcggtcaag
     1021 cagacctgga agatcgcgca ggcccgcctg ccggggcgcg tctactactg ggagctgccg
     1081 agccagtgcg gcaacaacaa cggccgcgtg ctcagcatca agccgaccga ggccaaggcc
     1141 gaggagttct tcaagcccgg ccagtgttac ggctgtcact cggtcagccg cgacggtcgc
     1201 acggtgtcgg tcgcgtacga caccggcgtg cccttcccgc tcggcacgat cgacgtgtcg
     1261 gccgagccgg cggtgcaggg cccgatcacg cccaacagcg ggctcgccgg gaccttcagc
     1321 gccttcaacc acgacggcac caagctgctg ttctccaacg acgcggtcaa cgcggccgcc
     1381 tcggggctgg tgatcgcgga cacgatgacc gcgcaggtgc taaaccccaa cgtgatgggc
     1441 tcgggctgcg gcgagccggc ctggtcgccc gacgggacgt tgatcgccgc gacctgcggg
     1501 tacaccgact tcggctggac cttcgacgcg cagacggccg agctgcggat cgccaccgtc
     1561 aaccccgacg gcatcaccgt ctcggacatc aagacgatcg tgccgcagat gggcgacgtc
     1621 ggccgccccg cgtacccgaa cttctcaccc ggcaacgagt ggatcgtgta cgggcgcccg
     1681 acgcagggct cgcgctcgac cggcaacggc aagctgtaca tggtcggcgt cgacggccaa
     1741 aataaccgca cgctcgagat cgccagctcg gacaacaaga gcttcaaccc gaccttcgcg
     1801 ccgctgcgcg cgggcggcta cttctgggtc gtgttcatga g
//
LOCUS       DASWNJ010000017         1844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_424257, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000017 DASWNJ010000000
VERSION     DASWNJ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424257"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1149)
                     /locus_tag="VGB85_00370"
     CDS             complement(121..1149)
                     /locus_tag="VGB85_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family protein"
                     /protein_id="HEY0132487.1"
                     /translation="MMQPRTDWAETIPPGEDAALLRLAEILAALQARRARKGRLGRAL
                     HFKGHGGVRAEVRTRDGLPAPYAVGIFAGAATYPAYVRFSNATGKPQPDRLGDVRGFA
                     LKIVGVPGKKLIPGMEDATTQDFLLIRTPVTPFRSAEEFVRMIQVVDNPLMLFAAMAR
                     LGVGRVLTIARQAAASMKSPTTTLATPRFYSALPIRWGEHAGRVSLTPVPPLDERTHG
                     DGGPDALRGDLAARLKAGPIAFTLAVQLYVDPQRTPIEDGSVDWSEDDSPYVPVADVV
                     LPQQDLTDDRGRELDAYVEQLSFDPWHAPVEFRPLGNLMRARNHAYRLSTTARKAGPE
                     PDGSETFL"
     gene            1362..>1844
                     /locus_tag="VGB85_00375"
     CDS             1362..>1844
                     /locus_tag="VGB85_00375"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEY0132488.1"
                     /translation="MKPRARDLGVGFSGTPGPLDAITDVAGVSVGYTTIVRGEGPLVV
                     GQGPVRTGVTAIHPRGLASHEPVFAGWCALNGNGELTGTTWIDESGFLEGPVLLTNTH
                     SVGVVSAAVVAWQARTGRMIQPYSYPVVGETYDGRLNDINGFHVTAAHALAAIEGASE
                     G"
BASE COUNT          258 a          616 c          701 g          269 t
ORIGIN      
        1 gccgaagttc gtcgacatgc tggcggccca cggcgtgctg ctgcaagtcg gccggttcgg
       61 cgagctcaag cgccagccgg cgcgcgcgag ctgaggcgcc tgtccccggg gacaggtgcc
      121 ttatagaaaa gtctcgctgc cgtccggctc ggggccggcc ttgcgggcgg tggtgctgag
      181 ccggtaggcg tggttgcggg cgcgcatcag gttgccgagc gggcgaaact cgaccggcgc
      241 gtgccagggg tcgaaggaga gctgctcgac gtaggcgtcg agctcgcggc cgcggtcatc
      301 ggtgaggtcc tgctgcggga ggacgacgtc ggcgaccggc acatacgggc tgtcgtcctc
      361 cgaccagtcg accgatccat cttcgatcgg ggtgcgctgc ggatcgacgt acagctggac
      421 cgcgagggtg aacgcgatcg gtcccgcctt caggcgcgcg gccaggtcgc cgcgcagcgc
      481 gtcgggtccg ccgtcgccgt gcgtgcgctc gtcgagcggc gggacgggag tcagcgagac
      541 gcggccggcg tgctcgcccc agcggatcgg cagggcgctg tagaagcgcg gcgtcgccag
      601 agtggtcgtc ggcgacttca tcgaggcggc ggcctggcgg gcgatcgtca gcacgcggcc
      661 gacaccgagg cgggccatcg cggcgaacaa catcagcgga ttatcgacga cctggatcat
      721 gcgcacgaac tcctcggcgc tgcgaaacgg ggtcaccggg gtccggatca gcaggaagtc
      781 ctgagttgtc gcgtcctcca tgccggggat caacttcttg ccgggcacgc cgacgatctt
      841 gagcgcgaac ccgcggacgt cgccgagcct gtcgggctgc ggcttgccgg tggcgttcga
      901 gaagcggaca taggccggat aggtcgcggc cccggcgaag atgccgacgg cgtacggggc
      961 cggcagacca tcgcgcgtcc ggacctccgc gcgcacgccc ccgtgaccct tgaagtgcaa
     1021 cgcgcgaccg agccggccct tgcgggcgcg cctggcctgc agcgcggcga ggatctccgc
     1081 caggcgcagc agcgccgcgt cctcgcccgg ggggatggtc tcggcccagt cggtacgcgg
     1141 ctgcatcatg cgcccgagcc taacccgaag cggtcgcgtc atgacgccgc cggttttgcg
     1201 ccgccgcgtc gtcccggtcg gcggcgagga tatgcgaaaa cgaccgccgc gggcgcggcg
     1261 tgttagtgtg cgccgatgtc ccgcctgtcc ctcgtcctcg tggtcccgct cctctcctgt
     1321 ggtgatagcg gcctcgaccg acccgtgcac gaggagcaac gatgaagcca cgcgcgcgcg
     1381 acctcggcgt cggattttcg ggtacaccgg ggcccctcga tgcgatcacg gacgtcgccg
     1441 gcgtcagcgt cggatatacg acgatcgttc gcggcgaggg gccgctggtg gtcggccagg
     1501 ggccggtgcg tacgggcgtc acggcgatcc atccgcgcgg gctcgcgtcg cacgagccgg
     1561 tgttcgccgg ctggtgcgcg ctcaacggca acggcgagct gaccggcacc acgtggatcg
     1621 acgagtcggg gttcttggag gggccggtgc tgctgacgaa cacgcacagc gtcggggtcg
     1681 tcagcgcggc ggtcgtcgcc tggcaggcgc gcacggggcg gatgatccag ccgtattcgt
     1741 atccggtcgt cggcgagacc tacgacgggc gcctcaacga catcaacggc ttccacgtga
     1801 cggccgcgca cgcgctcgcg gcgatcgaag gcgcgagcga gggt
//
LOCUS       DASWNJ010000018         5084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_424266, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000018 DASWNJ010000000
VERSION     DASWNJ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424266"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VGB85_00380"
     CDS             <1..244
                     /locus_tag="VGB85_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEY0132489.1"
                     /translation="LETLAPFIDFGASPRASIALFQAARAHAFMRHRGYVSPEDVKAV
                     APDILRHRIILSYEAEAEGETVEHVVRKILEHVQVP"
     gene            254..1231
                     /locus_tag="VGB85_00385"
     CDS             254..1231
                     /locus_tag="VGB85_00385"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY0132490.1"
                     /translation="MARTTADSRAEQAREEAKARTAQLLREIRRIEIQTSRLVNQHLA
                     GSYQSVFKGQGVAFSEVRGYIPGDDVRTIDWNVTARTGEPHVKLFAEERELTVMLVVD
                     MSASLDLGTRDFDKRNLVARLAATFAFSAIRNNDRVGLIGFTDEVEVFVPPRSGRKHV
                     LAVVQQLLTHETRARGTRPAAGLEYLARLARGHTVAILISDFVDVLPEEKSRADEARA
                     FEHALKVVRRRHDLIPIRVEDPMELDLPALGLVAFEDLELLGLGGEYFVDMSKGRANR
                     YRKQVAAERERVDTLLRKLRLDGMSIRCGDDWQKPLVHFFARRARRMRR"
     gene            1228..2508
                     /locus_tag="VGB85_00390"
     CDS             1228..2508
                     /locus_tag="VGB85_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132491.1"
                     /translation="MTAHLLGLLLALAPVPTNGPAGPPPEAPVPTDSRAVAARADAPV
                     PGDSRAAPVPSDIPAVAPAGAPVPLADEPAEPPAAPAQPAAPAQPPVVTPAEPPIAAP
                     TQPSAGSPATAPDGPVAVTAKIEPDPSQVGDLIKLELTAAYPAGFTVNLPLGLHFEPL
                     HLVSVDEAEPESTGQGFRKRFTITLQHFAVGEAKVPAFPLTYLAPDGAVQTVQVPPTP
                     FTVSSLLANEPDPKRQGEDPPVSLEYPNRRAEIIIYSAAAALLVGLVGGLLFARWRRR
                     PKPIVLPPPIPAHVVAFQQLDELQRGELVARGEYQDYYLQLTEIAKGYLEGRFGIDAL
                     DRTTDELRRALERAAAQIAPLKPTELVRFLQACDLVKFARFAPPGEEAQAALEQVRSM
                     VAATAPGSDPKDSKPADASPPAPPAPPSTPEATA"
     gene            2505..3533
                     /locus_tag="VGB85_00395"
     CDS             2505..3533
                     /locus_tag="VGB85_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0132492.1"
                     /translation="MSAVLPILGFAIVALLLALASVALKLGGDLRFIVLDSPWWLLTA
                     LVPLLAIALRAFLAPRPPTLQFSRTRSARSLGPGLAARLAHLPDGLRLAAALLLAFAL
                     ARPQSSRLTDRIEHEGIDIAIALDLSESMEADDLFPNRLEAAKEVIDQFIARRPHDRI
                     GMVAFGAYASTVSPLTVDHGVLRQLVRRLQLGGIDGSKTAIGAGLGLALNRLKESDAE
                     TQVIVLLTDGVHNAEGIDPDSAAAEAKEREIEVYTVLIGRHASGQSVDPGRLERIAGL
                     TGGYAYTAEDQQALITTFQDLLNKLEKSTIEGEHVRAELFFLFLWPALILLLLDVLLR
                     STRLRRFP"
     gene            3530..4672
                     /locus_tag="VGB85_00400"
     CDS             3530..4672
                     /locus_tag="VGB85_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0132493.1"
                     /translation="MILAPAPVAPEAVASAPADTVLSLFDTLVLGRGDLMWLLVLVPV
                     VAGAYVWATRQRRAALLRLGNQTLVSRLVGTVHRGNRLVIAVVTTIAALFLVGALMRP
                     QYGGTTRIVPVGGLDVVLVVDYSKSMLAKDVYPSRSERLAAELRRFLDESERRGDQVG
                     LVVFAGAARGLPVSRDTRVLKLYLDKADPHTENPGGTAIGKALKLALQYLVDARKAGQ
                     TRAQEAAGEAPPVEADQVIILLTDGEDTSSRPTEMAAEAAKLGVRIYTVGIGSKSGEP
                     IQKFDENGQPNGYQTDDKGNYLMTRVDEDSLKKLAEATGGQHVLVEPERFGLDAVSEW
                     IRDLTRGQRADTVTLDREEGYPFLVVPALLLLSLSLALGERRRHKP"
     gene            4669..>5084
                     /locus_tag="VGB85_00405"
     CDS             4669..>5084
                     /locus_tag="VGB85_00405"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132494.1"
                     /translation="MIAAGSKTLLALVAAGWLAEALASTHGDIDAGVAAYKAGDSEAA
                     LSSYAAAEADLGEQPEIHFNKGLALLAKDDKEGARKAFEHGTESEHAAVAASAEYELG
                     NLELDAEAFDAAIDRYIKCLKLQPDHASAKWNLELAL"
BASE COUNT          751 a         1969 c         1652 g          712 t
ORIGIN      
        1 cctcgagacc ctcgcgccgt ttatcgactt cggcgcctcg ccgcgcgcga gcatcgccct
       61 gttccaggcc gcccgcgcgc acgccttcat gcgccaccgc ggctacgtca gcccggagga
      121 cgtcaaggcc gtcgcgcccg acatcttgcg ccaccgcatc atcctctcct acgaggccga
      181 ggccgagggc gagaccgtcg agcacgtcgt ccgcaagatc ctcgagcacg tccaggtccc
      241 ctgacattgg cccatggccc gcaccaccgc cgatagccgc gcggagcaag cccgcgagga
      301 agccaaggcc cgcacggccc agctgttgcg ggagatccgt cgtatcgaga tccagacctc
      361 gcggctggtc aaccaacacc tcgctggctc gtaccagtcg gtgttcaagg gccagggcgt
      421 cgccttctcg gaggtccgcg gctacatccc cggcgacgac gtgcgcacga tcgactggaa
      481 cgtcaccgcg cgcaccggcg agccgcacgt caagctgttc gccgaggagc gcgagctgac
      541 cgtgatgctc gtcgtcgaca tgtcggccag cctcgacctc ggcacccgcg acttcgacaa
      601 gcgcaacctc gtcgcccgcc tcgccgccac cttcgccttc tctgcgatcc gcaacaacga
      661 ccgtgtcggc ctgatcggtt ttacagacga ggtcgaggtg ttcgtgccgc cgcgcagcgg
      721 tcgcaagcac gtgctcgcgg tcgtccagca gctgctcacc cacgagaccc gcgcccgcgg
      781 cacgcggccc gccgccggcc tcgagtacct cgcccgcctc gcgcgcggcc acacggtggc
      841 gatcctgatc tccgacttcg tcgacgtgct gccggaggag aagtcgcgcg ccgacgaggc
      901 gagggccttc gagcacgcgc tcaaggtcgt gcgccgccgc cacgacctca ttccaattcg
      961 cgtcgaggac ccgatggagc tcgacctgcc cgcgctcggc ctcgtcgcct tcgaggacct
     1021 cgagctgctc ggcctcggcg gcgagtattt cgtcgatatg agcaagggaa gagccaaccg
     1081 ctaccgcaag caggtcgccg ccgagcgcga gcgcgtcgac acgctgctgc gaaaactccg
     1141 cctcgacggc atgtcgatcc gttgcggcga cgactggcaa aagcccctcg tccacttctt
     1201 cgcgaggcga gcccggagga tgcgccgatg accgcccacc tgctcggact gctgctggcg
     1261 ctggcacctg tcccgacgaa cggccccgcc ggcccgcccc cggaagcgcc tgtcccgacg
     1321 gacagccgag ccgtcgcggc gcgcgcggac gcacctgtcc ctggggacag ccgcgccgca
     1381 cctgtccctt cggacatccc cgcggtggcc ccggcgggcg ccccggtgcc tctggcagac
     1441 gagccggcgg agccacccgc tgcgcccgcg cagcccgctg cgcccgcgca gccccccgtc
     1501 gttacgcccg ccgagccgcc catcgcagcg cccacgcagc cgtccgccgg atcgcccgcg
     1561 accgcgccgg acggcccggt cgccgtcacc gcgaagatcg agcccgaccc ctcgcaggtc
     1621 ggagacctga tcaaactcga gctgaccgcg gcatatccgg ccggcttcac cgtcaacctc
     1681 ccgctcggcc tgcacttcga gccgctgcac ctcgtctcgg tcgacgaggc cgagcccgag
     1741 tcgaccgggc agggctttcg caagcgcttc acgatcacct tgcagcactt cgcggtcggc
     1801 gaggccaagg tgcccgcgtt cccgctgacc tacctcgcgc ccgacggcgc cgtgcagacg
     1861 gtccaggtcc cgccgacgcc gttcaccgtg tcgagcctgc tggccaacga gcccgacccg
     1921 aagcgtcagg gcgaggaccc gccggtgtcg ctcgagtacc cgaaccgccg cgccgagatc
     1981 atcatctaca gcgccgcggc ggcgctgctc gtcggcctcg tcggcggcct actgttcgcc
     2041 cgctggcgcc ggcgcccgaa gccgatcgtg ctgccgccgc cgatcccggc gcacgtcgtc
     2101 gccttccagc agctcgacga gctgcaacgc ggcgagctcg tcgccagggg cgagtatcag
     2161 gactactact tgcagctgac cgagatcgcc aagggttacc tcgagggtcg cttcggcatc
     2221 gacgccctcg accgcaccac cgacgagctg cgccgcgccc tcgagcgcgc cgcggcccag
     2281 atcgcgccgc tcaagccgac cgagctggtg cgcttccttc aggcctgcga tctcgtcaag
     2341 ttcgcccgct tcgccccgcc gggcgaggag gcccaggccg cgctcgagca ggtccgttcg
     2401 atggtcgccg cgaccgcgcc cggatctgac cccaaggaca gcaagcccgc cgacgcaagc
     2461 ccgccggctc caccagctcc gccgagcacg ccggaggcga ccgcatgagc gccgtgttgc
     2521 cgatcctcgg gtttgcgatc gtggccctgc tgctcgcgct cgccagcgtg gcgctcaagc
     2581 tcggcggcga cctgcgcttc atcgtgctcg actcgccgtg gtggctgctg accgcgctcg
     2641 tgcccctgct ggcgatcgcc ctccgggcct tcctcgcacc gcggccgccg accttgcagt
     2701 tctcgcgcac tcgatcggcg cgcagcctcg ggcccgggct cgccgcgcgc ctcgcccacc
     2761 tgccggatgg gctgcgcctc gcggccgcgc tgctgctggc cttcgcgctc gcgcgcccgc
     2821 agtcgagccg cctgaccgac cgcatcgagc acgagggcat cgacattgcg atcgcgctcg
     2881 acctcagcga gtcgatggag gccgacgacc tgttcccaaa ccggctcgag gccgccaagg
     2941 aggtgatcga ccagttcatc gctcgccgcc cgcacgaccg catcggcatg gtcgccttcg
     3001 gcgcctacgc ctcgaccgtc tcgccgctga cggtcgacca cggcgtgctc cgtcagctgg
     3061 ttcggcgcct gcaactcggc ggcatcgacg gctcgaagac ggcgatcggc gcgggcctcg
     3121 gcctcgcgct caaccgcctc aaggagtccg acgccgagac ccaggtgatc gtgctgttga
     3181 ccgacggtgt gcacaacgcc gagggcatcg accccgactc cgccgcggcc gaggccaagg
     3241 agcgcgagat cgaggtgtac accgtgctga tcggccggca cgcgagcggc cagagcgtcg
     3301 accccggccg gctcgagcgc atcgccggcc tgaccggcgg ttatgcatat accgccgagg
     3361 atcagcaggc cctgatcacc accttccagg acctcctcaa taaactcgag aagtcgacga
     3421 tcgagggcga gcacgtgcgc gccgagctgt tcttcttgtt cctgtggccg gcgctgatcc
     3481 tgctgctgct cgacgtgctc ctgcgctcga cccggctccg gaggttccca tgatcctcgc
     3541 gcctgcaccc gtggcgcccg aggccgtcgc ctcggcgccc gccgacacgg tgctcagcct
     3601 gttcgacacc ctcgtgctcg gtcgtggcga cctgatgtgg ctgctcgtgc tcgtgcccgt
     3661 ggtcgcgggc gcgtacgtgt gggcgacccg ccagcgccgc gccgcgctgc tgcgcctcgg
     3721 gaatcagacc ctggtgtcgc gcctcgtcgg caccgtgcac cgcggcaacc gcctcgtcat
     3781 cgccgtcgtc acgacgatcg cggcgctgtt cctcgtcggc gcgctgatgc ggccgcagta
     3841 cggcgggacc acccgcatcg tcccggtcgg cggcctcgat gtcgtgctcg tcgtcgacta
     3901 cagcaagtcg atgctcgcca aggacgtgta cccctcgcgc agcgagcggc tcgcggccga
     3961 gctgcggcgc ttcctcgacg agtccgagcg gcgcggggac caggtgggcc tggtggtgtt
     4021 cgccggggcc gcgcgcggcc tgccggtcag ccgcgacacg cgggtgctca agctctacct
     4081 cgacaaggcc gacccgcaca ccgagaaccc gggcggcacc gcgatcggca aggcgctcaa
     4141 gctcgcgttg cagtacctcg tcgacgcgcg caaggccggg cagaccaggg cccaggaggc
     4201 cgccggcgag gccccgccgg tcgaggccga ccaggtgatc atcctgctga ccgacggcga
     4261 ggacaccagc tcgcgcccga ccgagatggc cgccgaggcc gccaagctcg gcgtacggat
     4321 ctacacggtc ggcatcggct cgaagtccgg cgagccgatc cagaagttcg acgagaacgg
     4381 ccagccgaac ggctaccaga ccgacgacaa gggcaactat ctgatgaccc gcgtcgacga
     4441 ggacagcctc aaaaaactgg ccgaggcgac cggcggacag cacgtgctcg tcgagcccga
     4501 gcgcttcggc ctcgacgccg tgtccgagtg gatccgtgat ctgacgagag ggcagcgcgc
     4561 cgacactgtc acgctcgacc gcgaggaggg ctaccccttc ctggtcgtgc cggcgctgct
     4621 gttgctctcg ctctcgctcg cgctcggcga gcgcaggagg cataagccat gatcgcagca
     4681 ggcagcaaga ccctcctcgc gctggtcgcc gccgggtggc tggccgaggc cctcgcgtcg
     4741 acccacggag acatcgacgc cggcgtcgcc gcctacaagg ccggtgacag cgaggcggcc
     4801 ctgagcagct acgccgccgc cgaggccgac ctcggcgagc agcccgagat ccattttaat
     4861 aaggggctcg cgttgctcgc caaggacgac aaggagggcg cgcgcaaggc cttcgagcac
     4921 ggcaccgagt cggagcacgc ggccgtggcg gccagcgccg agtacgagct cggcaacctc
     4981 gagctcgacg ccgaggcctt cgacgccgcg atcgatcgct acatcaagtg cctcaagctc
     5041 cagcccgacc acgcgagcgc caagtggaac ctcgagctcg ccct
//
LOCUS       DASWNJ010000019         2666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_283109, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000019 DASWNJ010000000
VERSION     DASWNJ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283109"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VGB85_00410"
     CDS             complement(<1..235)
                     /locus_tag="VGB85_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132495.1"
                     /translation="MIVERHVDDDMLASLLLFPYPDDPRAETTQPVVLADPIQTHRLE
                     LVEGSNLVYALSPEGELLRADLGDQSVTSVLPDV"
     gene            978..2285
                     /locus_tag="VGB85_00415"
     CDS             978..2285
                     /locus_tag="VGB85_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0132496.1"
                     /translation="MRPSSLVPTLAGGWPFLGHLPEMHLDFPGLCARGVAAHGLLFWI
                     TPGPGGGQLMCADPAIVEALKGRQVSVEVYAENIGAVLGGTVLALDGEAHRQLRGTIA
                     PPFTPKQVRRSDIVAVIAEVARAHVARWLQAGRIEVLPATQELALEIIVRLIGVPPRN
                     LAAWGRQYRRYMLSLLPSRLPTPTHWWARRARRWLDRGMREIVAELRAAGDDTTLVGG
                     LANARDEHGALLPVDRLIPNLLLLALAGHETTANSLAWAILEQARDPDSQARARVEVE
                     GADLTALALDSEQFTWAERQFREALRLYPPVHSLVRKLVAPLTIAGVELPAGTLINVP
                     VAHFLRDPAYGADPGAYDPARFTTRPRISTIDTIMFGGGPHFCLGYHVAIAEGSLVLL
                     TLARALAEAGLRVVPEFTDAALRPRWLPVAHPPSKLAVRFTTA"
     gene            2449..>2666
                     /locus_tag="VGB85_00420"
     CDS             2449..>2666
                     /locus_tag="VGB85_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132497.1"
                     /translation="MITDLSLGTDRCGSRPREVVRRVVTIEPVADREQGGRRSDVMKH
                     CSRAGEAARYAGNRIWYLRWLVRAALER"
BASE COUNT          403 a          968 c          921 g          374 t
ORIGIN      
        1 ccacgtcggg cagcacgctg gtgacgcttt gatcgccgag atcggcgcga aggagctctc
       61 cctccggaga caaggcgtag accagattcg agccctcgac gagctccagc cgatgggtct
      121 gtatcgggtc ggccaggacg acgggctgcg tggtctctgc tcgcggatcg tccggataag
      181 ggaacagtag cagcgaggcg agcatatcat cgtccacgtg gcgctcgact atcacggccc
      241 cgtggtccgt ccatacgggg gaacagctac gggcggaaaa taccgagtgg ggggcgagca
      301 ggcccgtggg atcgagggtc acaagaccat cgtccaaagc gcacgcgaac gcgacgccag
      361 gccagcgctc gacagtctgg ggagcggcag ccccgtcagc gatgcgagtc ggcgactccc
      421 cgcagagccc ggtcgaccat atcgaataat cgaatccagc cagcggtctc cccggctgca
      481 cacgaaagac accatgataa agccggcccg cgatggcgat cgtatgcgcc cgctgatctg
      541 gttccagcgc caacacctgg acggggccca tggccccgca caactcggtg aacacgaccg
      601 gcccgggccc atcatcggtg caaccggcca gaccactcac caacaacaga accatagcat
      661 cccgcgccat cttcgcagga tacccccggc ggccaggttg ccgccagcgc tcgtaacgag
      721 gcgccaccgc ggagccactc cccgtccgac gcgaagcgag gcgcgacccc gcggacctcg
      781 ggcgccgcgt agccgatgtt ctgcaagggt ttgccgcgct ttggctcggc gcgttcctcc
      841 gacggcgtcg cccgctcggt cagggccgcg taagacccgg cgcttcggcg agcagcaagc
      901 gttggcccgc atcgagctcg ggctggcgaa acctgcttcg tggaattcgt gcgggcgcgg
      961 gggtataagc tggggcgatg cgtccctcga gcctcgtccc gaccctggcc ggcgggtggc
     1021 cgttcctcgg gcacctgccg gagatgcacc tcgacttccc gggcctgtgc gcacgcggcg
     1081 ttgcggcgca cggtctcctg ttctggatca cgcctggacc cggcggcggc cagctgatgt
     1141 gcgccgaccc ggcgatcgtc gaggcgctga aggggcgcca ggtctcggtc gaggtctacg
     1201 ccgagaacat cggcgcggtg ctcggcggga cggtgctggc gctcgatggc gaggcgcacc
     1261 ggcagctgcg cgggacgatc gcgccgccgt tcacgccgaa gcaggtgcgg cgctcggaca
     1321 tcgtggcggt catcgccgag gtcgcgcgcg cccacgtcgc ccgctggctg caggcggggc
     1381 gcatcgaggt gctgccggcg acgcaggagc tcgcgctcga gatcatcgtc cggctgatcg
     1441 gcgtgccgcc gcgtaacctc gccgcctggg gccgacagta ccggcgctac atgctgagcc
     1501 tgctcccgtc ccggctgccg acgccgacgc actggtgggc ccgccgcgcc cgccgctggc
     1561 tcgaccgcgg catgcgagag atcgtcgcgg agctgcgggc cgcgggcgac gatacgacgc
     1621 tcgtcggcgg gctcgccaac gcccgcgacg agcacggcgc gctgctgccg gtcgaccggc
     1681 tgatccccaa cctcctcctg ctcgccctcg ccggccacga gacgaccgcg aacagcctcg
     1741 cctgggcgat cctcgagcag gcgcgcgacc ccgacagcca ggcgcgggcc cgcgtcgagg
     1801 tcgagggcgc cgacctgacc gcgctggccc tcgacagcga gcagttcacg tgggccgagc
     1861 gtcagttccg cgaggctctg cgcctgtacc cgccggtgca ctcgctggtc cgcaagctcg
     1921 tcgcgccgct gacgatcgcc ggcgtcgagc tccccgccgg cacgctgatc aacgtgccgg
     1981 tcgcccactt cctccgcgac ccggcgtacg gcgcggaccc cggcgcgtac gaccccgccc
     2041 ggttcacgac gcgcccgcgg atcagcacca tcgacacgat catgttcggc ggcggcccgc
     2101 acttctgcct cggctaccac gtggcgatcg ccgagggctc gctggtcctg ctgacgctgg
     2161 cccgcgcgct ggccgaggcc gggctgcgcg tcgtccccga attcacggac gcggccctgc
     2221 gcccgcgctg gctgccggtc gcgcacccgc cgagcaagct cgccgtccgc ttcacgacgg
     2281 cgtgacttgc tcgtgtgaag agcagcgagg tccgaagagc aactctggac ccctatcatc
     2341 aaggacgcgg tgggcgtcgg cgaggaggtc gccgacgtca aacttcaatg gccgcgtgcg
     2401 cgcgagaacg cagcaccgta aggcgtggct ccgtggtggc caggccgcgt gatcaccgac
     2461 ctgtccctcg ggacagaccg ctgcggctcg cggccgcgcg aggtcgtgcg ccgcgtcgtc
     2521 acaatcgagc cggtggccga ccgcgagcag ggagggcggc gctccgatgt catgaaacat
     2581 tgttcccggg ccggcgaagc cgctcgatat gccggcaatc gcatctggta tcttcgctgg
     2641 cttgtccgcg ctgccctcga acgaca
//
LOCUS       DASWNJ010000020        15485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_494880, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000020 DASWNJ010000000
VERSION     DASWNJ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 15485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15485
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494880"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VGB85_00425"
     CDS             <1..512
                     /locus_tag="VGB85_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132498.1"
                     /translation="AEALGHPRQDIETTLEAMRQAGVLVAIGGGAVTRYGVTPEYKAE
                     LEAEFRAAAAELGEHAMLARLSSESALVPFEDEEPRLPATAGLLSLFLPGTGQLLNGD
                     IGRASLVFAVWSLAWITHLSPVWTFVCLYAGAEAFLTAKIRSMERKLADEQGTMASGP
                     KKLPAPRLT"
     gene            complement(505..1776)
                     /locus_tag="VGB85_00430"
     CDS             complement(505..1776)
                     /locus_tag="VGB85_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132499.1"
                     /translation="MSPARKTLLWMAGLSLVTLLLGVLREFWIARELRASGEADLFFR
                     GLVIVGAARAVGNSLFRSRWIPIPMHTAATALLRAEAGTMAGMTGVALVSLWAIAGVS
                     ALRDPTLWVFAVAVPLAIVGSAIRALAERAGLERRGFVLEWALPLGAIAGALLVPHGA
                     LGPALGILAGLIVGLAALVPPVLAGGADLAHGTGPVGQAPPVLAGAPAGADLARGTGL
                     KGQVIVSEPGRMRVLLLDAMVYANLGLLDAALSHLFADGGFALLNYSYLFVNAALMVP
                     SAAATVVGLRTAASGDPDAHAVLRRWAVLGGLVCAGLVVVVGLMLGWGPVAREVDAAV
                     GWSLAASIGGLVMWSAPYAGLRLANTIGRQYVIASEPRRVIMWDLTGLLGRALILGFG
                     AAVLGLVASPIGLAFAEVLQLGAWWRQPKLR"
     gene            complement(1773..2462)
                     /locus_tag="VGB85_00435"
     CDS             complement(1773..2462)
                     /locus_tag="VGB85_00435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HEY0132500.1"
                     /translation="MTGRRTFLKKLLTKIGARLSAGAIHRLNAAINYLEVGRWMAAHG
                     FDTSKRVLDRRETYAKIAAELGDVPVLYLEFGVHRGESIGVWSDLLKNTGSQLHGFDS
                     FEGLPEDWTLHDGKGTFSTGGQVPVFADSRVKFFPGWFDTTLKTYELPAHERLVIHLD
                     ADLYSSTKTVLDALERHIVPGTYLLFDEFSDRLHELRAFDEFLRMHPGWRFRLVGADA
                     ILARVAFERVS"
     gene            complement(2459..3343)
                     /locus_tag="VGB85_00440"
     CDS             complement(2459..3343)
                     /locus_tag="VGB85_00440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0132501.1"
                     /translation="MSEAAARAPFAVVMLTYNEEINLPKALASIGTRAPVIIVDSEST
                     DGTAQVAREHGAEFVVHPFVDYASQRNFALEQAADRYRWIFFLDADEEFSKELWDEVL
                     ATVAEDQVDGAYVRLDVRVLGHRLMHGEFSSSMVLRLMRPEKARFGRGINERVDDREL
                     RIKVLRTRMIHRDARPLTHLFTKHIGYARKEALAYLDGVDRKRGLQGFGLRTKAARMI
                     GIRWAYNKMPLFVRPFALNFRAVVVQGAWRDGIPGLMHAGMHALWYPMLIDLLIYEEK
                     LRRAGVLAREFAPREIDR"
     gene            complement(3340..4473)
                     /locus_tag="VGB85_00445"
     CDS             complement(3340..4473)
                     /locus_tag="VGB85_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132502.1"
                     /translation="MLTTLFAAAAMLLTLALPTFPDGLTIPIVGFLGLVMLAGLGGFV
                     RSAAARAELALLAPWLVALGLSLMVGILRGNSLAQALEDALPYVLFAMGLCAGRGAAK
                     PRWVLATVLAVCLTDGLISLVRMPSFDLGRLRSTYYHFKVIAGHALVGVYCAALLYRQ
                     SRGKVHRWALMAVQAVLVLSVIATVSRGMVLGLVLGSLTALYVRRPARGLLVGGVAGL
                     LVLLFTASAFDLGAQYLRLGNQATVDGRVREIGLCLEYFTRMPVFGAGLGADFVVDGF
                     VVSYVHNMLAYHLWKFGLVGSALFALPLWGLTRQALRAPKLLRATVLGGAVSVLVYLV
                     TAAAYKSYFLVPMVGMVVGASLRMLSEIAALPAAAKPPSVVKT"
     gene            4932..7886
                     /locus_tag="VGB85_00450"
     CDS             4932..7886
                     /locus_tag="VGB85_00450"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY0132503.1"
                     /translation="MHRQWLDWHRPLLPAAAAWLIGQHSDGKRCDLRQVRCVLPGTRA
                     GRLLLHALLAQCAEQGLRLVPPQVLTPGTMVDQLVPPTPDTASALECTLGWMHALRDA
                     GIDEIASLLPSRPDSQDWPAWHELAGQIGRIVEELAGELLSLRDVARVAGRMDMEREA
                     QRWAVLERLADRYRERLRRCGRVDPHERRREAVASLVDADVPVDPGYQLVLVGVVDLA
                     RVQRAVVRAYGERATALIHAPDMLAHGFDADGCIERTFWTGRIVHVPDEQLIIADRPS
                     DQAQAVVECLAKLGGYFRPEQISIGLGDDKLGPAMAQAGEWADLTVHDPRGHVVLRSR
                     PFRLLDAGLEWVRDPRFGAFASLIRHPDVEAWVRRCLGARKTTATAEAPATEAPAEPA
                     PAAESPPALTVVPDAPTTEATAPVQEDMPILEWLTVLDRYYTEHLQDTGGGPWLGDEK
                     TVRRMEILDDIVRKLFAPLRGERRPLTEWFEPVLEVLRLAYGHIDVGTQQISEARVAA
                     TCLEIARTLGGLTGAAAELQPRVDGPTALRLVLAQIAGLRIPEDPRSGQVEMLGWLDL
                     HHDPAPVLILAGFNDGAIPQAVSADAFLPDGLREHLGILSNGRRYARDIYLLEAIRHS
                     RERCTFIAGRQAADGEPLTPSRLLLAGEQHLLPARVLKLCDPQHALRRNLPYGAPPSG
                     ATSGFRIPEVPTAPVPPEAMTVTEFGWYLRCPYRYYLKYIRRLRQVEDSAVELDALQF
                     GNLTHEVLQHFGEDPDHSRSTDPVRIASFLEEILVDRARAGFGAQPLPAIRIQLARLR
                     ERLRTFARYQARRSSEGWVIRRCEFELPEATYLEIPDQEKMRIKGKIDRIDQNERDGS
                     WMIIDYKTSEAGKTPDRTHKASRSSGRVWDDLQLPLYHHLAGQHGINGPVQLAYMNLP
                     KKAEQETLHVARWSAADLDDAIGTARDIVRNIRAGRFEMADDYPARFEDAFANICQTR
                     VFGGGDDLETEA"
     gene            7883..8047
                     /locus_tag="VGB85_00455"
     CDS             7883..8047
                     /locus_tag="VGB85_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132504.1"
                     /translation="MSARRNTSATPAAAASDGSAAPESLWLFSDSPIAPPPVPARVPK
                     TSQPGSPQQR"
     gene            8108..11668
                     /locus_tag="VGB85_00460"
     CDS             8108..11668
                     /locus_tag="VGB85_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY0132505.1"
                     /translation="MFARSSEAARAEIAGEAPPAPQARHRVPAEGPRPVPEARPAQPV
                     QKDTRPAPARGPADEPVPWDSDDGQWTDHGPDIDLGDLPDLPWSTPAGELEINLIPGG
                     SRTEVLALLREAEDVASSAMVFEDDRNLEGDASLQHVSILASAGSGKTYQLTNRYLQI
                     LARGAAPYGILASTFTRAAAGEIRDRILRTLAESAEVDDKRDELMRRLHRPGMSRQFV
                     LDMLTALAHNLHRLQIRTLDSFFGTIVRCFALELGLPADARIIDEDEAFRLRRAAIQR
                     MLEEGDEAAIIELLASLTEGSSERAVTDTIDNTVSSLYDLYREARYTDAWRAIAPGEC
                     LTEVALDAAIADLRAAVPSGPKRTIDCHAKDCERAEQCKTGSSDDWMEFFGQGLAAKI
                     ADGSCVYYAKPIDARTVAAYERLLKHAKGVYRRRVIDQTRATHELLLRFDDCYQRVKH
                     QHKALTFADLTVALAQADLSGKLDDICYRIDARLRHVLLDEMQDTSIQQWNAMRPIVD
                     ETVSYTPEERSFFCVGDVKQSIYGWRNACPEILSRIDQLLGAGAGIVKQNLATSYRSA
                     PAVIEVVNKIFTALANNPALMRYPDAAQRWDEEFQTHTTQRSALAGYVRLRTVARCED
                     QAKRQNLRLQEAAHLVHELYHRHPGKTIGVLLRTNGAVGRVLYELGPTRLKVPATGRG
                     GGPLTDAPAVNAILDLLRLADHPDHSVAAFNVLSSPLGPVVGLTEDGPGPRHRAAARV
                     RRMVASDGLSRTLQPWVKAIAGQVDERQYRRCMQLIQLAQAWDQRASLRCDDFVAMVE
                     TRAVADPSASAVQVMTIHQAKGLEFDIVILPELEAELASTRTLKVVYERNGDAGPISK
                     IARHVSKDMWSMFDDLRPMFEQHIGRLAHESLCLLYVAVTRAREGLFMLIDPPGESSR
                     TAPARMSSLVLHALHDGPADPDSVVFEWGDPQWLAPHAPVTAAPVDPSAPHLESPQAP
                     LTFAPTQGPLLRSTGAMASDLAGRTVGQVLVLNDGPTSAWGGAVRALFAEVTWLEDFV
                     EDRRALLAAVQKAAPNRDEEYAQTVVLEFLRMLTAATVRRALSRGGAPANALQVERQV
                     PFARLEAGALQTGEIPRVVLELDPNLPEGTEGHVRRATVLGFCLDNVDTQELAAERAL
                     SYREPMSAWRNATAEQWGIERDQVAGVLLFVVAGIAVPMT"
     gene            11674..12702
                     /locus_tag="VGB85_00465"
     CDS             11674..12702
                     /locus_tag="VGB85_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132506.1"
                     /translation="MGSDTLAVRIAGVHDTTLEFWAAELPQGPDGSFVSVIGWDHPAL
                     ALMLIAGSANHRNALAQALEGVRYYEREWLAEHTDEFVTSMTLIATARAEWHTNEWIA
                     GTIQQAGSSETSRALALQIAPRALYRVTVRDPAWLAHLRPGQEFGSTAYSPFPLVPRD
                     STGTMTTPGAWDPVLAVMRAPIDERRIHMYRNGLSPGSWCIDYESDRTAPSNRVRELF
                     LRLGEAQFVYFDATAPRFNRAKFPFAERKQLLAPAYKMWESGAAAGPRGFHLKSRVSQ
                     RHEDGVARMQLDDLCPGKRHAWLEVEGEPRVRFGDWSSVDPSSPSVDVDDYLRDVAER
                     VRLDLATP"
     gene            complement(12692..14974)
                     /locus_tag="VGB85_00470"
     CDS             complement(12692..14974)
                     /locus_tag="VGB85_00470"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY0132507.1"
                     /translation="MLVARRSFLAGLGIGAGGLALGFFKSGETWADEPEAGPPKPPTT
                     SSPTVEAKAPGLNPNVFVHVGLDGAVTIVCQRSEMGQGIRSTLPVLIADELGADMARV
                     KILQADGDPAYGDQNTDGSNSIRGIYEAMRRAGATPRTMLIAAAARRWKVDAGTCTAR
                     EHRVIHEKSGRTLGFGELAAEAAKLKVPEPHEVVLRPREELRTIAGQLPLIDAPAYVT
                     GAAIFGADVRLESMLTAVIARPPVVGGKVARYDATRTLAVPGVRQVIEIPAAEHPWAF
                     KPLGGLAVLADNTWAAMRGRAALDITWAHGDNASHGSSGYRQALIAALGEPGEVLRKI
                     GDAEAALASAAKVIEADYFVPHLAHVSMEPPVAVARFEGERCEVWAPTQHPQSARKEV
                     ARVLETTEDMVTVHVTFLGGGFGRKSKPDFVAEAALLAKQAGVPVRVQWTREDDVRHD
                     YFNTVSAQRLTAGLDAGGKLVAWRHRTAFPPIGSLFKDIDRPELNDLQQGVLDLALAA
                     PNVTAESCEAKAHVRVGWLRSVYNIFHAFAVGSFIDEIAQARGEDPRTTWLEVLGPPR
                     YMTIKELGVESLKNYGQPLEKHPVDAGRLRHVVERVTALSGWDTRKQDGKALGLAAHR
                     SFLSYAAVVASVVQGAGGKLVVDEVWLVIDAGIVINKDRVISQMEGAVIFGMSLALHS
                     AISLKDGAVEQSNFHDFRVTRIGEAPRKIHVEIVESDKAPGGIGEPGVPPVAPAIANA
                     LFALTGQRVRELPLGKTITV"
     gene            complement(14978..15433)
                     /locus_tag="VGB85_00475"
     CDS             complement(14978..15433)
                     /locus_tag="VGB85_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY0132508.1"
                     /translation="MKIRINGVERELEVDPEMPLLWALRDVLGLTGTKYGCGQALCGA
                     CTVHLDGQVVRSCVTPVGRAEGRTVTTIEGLSPAGDHPLQKAWCELGVPQCGFCQAGQ
                     IMTAAALLAVTPQPSDAEIDRSLAGNLCRCGTYTRIRAAVKRAAGVAGE"
BASE COUNT         2366 a         5973 c         4994 g         2152 t
ORIGIN      
        1 ccgccgaggc gctcggtcac ccgcgccagg acatcgagac cacgctcgag gcgatgcgcc
       61 aggccggcgt gctggtggcg atcggtggcg gcgcggtgac tcgctatggc gtaacgcccg
      121 agtacaaggc cgagctcgag gccgagttcc gggccgcggc ggccgagctc ggtgagcacg
      181 cgatgctggc ccgcctgtcg agcgagtcgg cgctggtccc cttcgaggac gaggagccgc
      241 gcctgccggc caccgcgggc ctgctgagcc tgttcctgcc cggcacgggt cagctgctca
      301 acggcgacat cggccgcgcc tcgctggtgt tcgcggtgtg gagcctcgcg tggatcaccc
      361 acctctcgcc ggtgtggacc ttcgtgtgcc tgtacgccgg cgccgaggcg ttcctgaccg
      421 cgaagatccg cagcatggag cgcaagctcg ccgacgagca gggcacgatg gccagcggac
      481 ccaagaagct cccggcgccg cgcctcacct gagcttcggc tggcgccacc acgccccgag
      541 ctgcagcacc tcggcgaacg cgagcccgat cggcgacgcg acgagcccga gcaccgccgc
      601 gccgaagccg aggatcagcg cgcgcccgag caacccggtc aggtcccaca tgatgacccg
      661 ccgcggctcg ctggcgatca catactgccg cccgatcgtg ttggcgaggc gcagcccggc
      721 atacggcgcg ctccacatca ccagcccgcc gatgctcgcc gccagcgacc agccgaccgc
      781 cgcgtcgacc tcgcgcgcca ccggtcccca ccccagcatc agtccgacaa cgacgacgag
      841 ccccgcgcac accagcccgc cgagcaccgc ccagcgccgc agcaccgcgt gcgcgtccgg
      901 gtccccgctc gccgcggtgc gcagcccgac caccgtcgcc gcggccgagg gcaccatcag
      961 cgccgcgttg acgaacagat acgagtagtt gagcagcgcg aagccgccgt cggcgaacag
     1021 gtgcgagagc gcggcgtcga gcagcccgag gttggcgtag accatcgcgt cgagcagcag
     1081 cacccgcatg cgcccgggct cactgacgat gacctgtcct tttagacctg tcccccgcgc
     1141 caggtctgcc ccggccggag cacccgcgag cactggcgga gcctgtccca cgggacctgt
     1201 cccatgcgcc aggtctgcgc cccccgcgag caccggcgga accagagccg cgagcccgac
     1261 gatcagcccc gcgaggatcc cgagcgccgg tcccagcgcc ccgtgcggca ccagcagcgc
     1321 cccggcgatc gccccgaggg gcagcgccca ctcgagcacg aagcctctac gctcgagccc
     1381 ggcgcgctcg gccagcgccc ggatcgcgct gccgacgatc gccagcggca ccgcgaccgc
     1441 gaacacccac agggtcgggt cgcgcaacgc cgacacccct gcgatcgccc acagcgacac
     1501 cagcgcgacg cccgtcatgc ccgccatcgt gccggcctcg gcgcgcagca gcgccgtcgc
     1561 ggccgtgtgc atcgggatcg ggatccaccg cgaacgaaac agcgagttgc cgaccgcccg
     1621 cgccgcgccg acgatcacga gcccacgaaa gaacaggtcg gcctcgccgc tggctcgcag
     1681 ctcgcgtgcg atccaaaact cgcgcaggac cccgagcagc agggtcacca gcgagaggcc
     1741 cgccatccac agcagggtct tgcgcgccgg gctcacgaga cccgctcgaa ggccacccgc
     1801 gccaggatcg cgtcggcgcc gacgagacgg aagcgccagc cggggtgcat gcgcaggaac
     1861 tcgtcgaagg cgcgcagctc gtgcaaccgg tcggagaact cgtcgaacag taaataagtg
     1921 ccgggcacga tgtgccgctc gagcgcgtcg agcaccgtct tcgtcgagga gtacagatcc
     1981 gcgtcgaggt ggatcaccag ccgctcgtgc gccggcaatt cgtaggtctt gagcgtcgtg
     2041 tcgaaccacc ccgggaagaa cttcacgcga ctgtccgcga acacgggcac ctgcccgccg
     2101 gtcgagaacg tgcccttgcc gtcgtgcagc gtccagtcct cgggcagccc ctcgaagctg
     2161 tcgaagccgt gcagctgcga cccggtattt tttaaaaggt ccgaccacac gccgatcgac
     2221 tcgccgcgat gcactccgaa ctcgaggtac agcacgggca cgtcgcccag ctccgcggcg
     2281 atcttcgcgt aggtctcgcg ccggtcgagc acccgcttcg aggtatcgaa gccatgcgcc
     2341 gccatccagc gcccgacctc gaggtagttg atcgcggcgt tgaggcgatg gatcgcgccc
     2401 gccgacaggc gcgcgccgat cttcgtcagc aactttttaa gaaaagttcg cctgccggtc
     2461 atcgatcgat ctcccgcggg gcaaactctc gcgcgagcac cccggcccgg cgcagcttct
     2521 cctcgtagat cagcaggtcg atcagcatcg ggtaccacag cgcgtgcatg cccgcgtgca
     2581 tgaggcctgg gatcccgtcg cgccaggcgc cttgcacgac gaccgcgcgg aagttgagcg
     2641 cgaacggccg gacgaacagc ggcatcttgt tataggccca gcggatcccg atcatgcgcg
     2701 ctgccttggt ccgcaggccg aagccttgca gaccgcgctt gcggtcgacg ccgtcgaggt
     2761 acgcgagcgc ctccttgcgc gcgtagccga tgtgttttgt aaagaggtgc gtcagcgggc
     2821 gggcgtcgcg gtggatcatc cgcgtgcgga gtaccttgat cctcaactcc ctgtcgtcga
     2881 cgcgctcgtt gatcccgcga ccgaagcggg ccttctcggg tcgcatcagg cgcaggacca
     2941 tcgagctcga aaattccccg tgcatcagtc ggtggccgag cacgcggacg tcgagcctta
     3001 catacgcccc gtcgacctga tcttcggcga cggtcgcgag cacctcgtcc cacagctcct
     3061 ttgaaaattc ctcatcggcg tcgaggaaga agatccagcg gtagcggtcg gcggcctgct
     3121 cgagcgcgaa gtttcgctgg ctcgcgtagt cgacgaaggg gtgcacgacg aactcggcgc
     3181 cgtgctcgcg ggcaacctgc gccgtgccgt cggtcgactc cgagtcgacg atgatcaccg
     3241 gcgcgcgggt cccgatcgac gccagcgcct tcgggaggtt gatctcctcg ttgtaggtca
     3301 gcatcaccac cgcgaagggg gcgcgcgcgg ctgcttcgct catgtcttca ccacgctagg
     3361 aggttttgcg gcggccggca acgcggcgat ctccgacaac atgcggaggc tcgcgccgac
     3421 gaccatcccg accatcggga ccaggaagta gctcttgtag gccgccgcgg tgaccaggta
     3481 cacgagcacg ctcaccgcgc cgccgagcac cgtggcgcgc agcagcttcg gcgcgcgcag
     3541 ggcctggcgg gtcagccccc acagtggcag ggcgaacagc gccgagccga cgaggccgaa
     3601 cttccacagg tggtaggcga gcatgttgtg gacgtaggag accacgaagc cgtcgaccac
     3661 gaagtccgcg ccgagccccg cgccgaacac cggcatgcgc gtgaagtact cgaggcacag
     3721 gccgatctcc ctgacgcggc cgtcgaccgt cgcctggttg ccgaggcgga ggtactgcgc
     3781 gccgaggtcg aacgcgctgg cggtgaacag cagcaccagc aggccggcga cgccgccgac
     3841 gagcaggccg cgggccggtc tgcgcacgta gagcgccgtc agcgacccca gcaccaggcc
     3901 gaggaccatg ccgcggctga ccgtggcgat caccgacagc acgagcaccg cctgcacggc
     3961 catcaaggcc cagcggtgga ccttgccgcg gctttgccga tacaggaggg cagcgcagta
     4021 cacgccgacc agcgcatggc cggcgatcac cttgaagtgg tagtaggtcg accgcagtcg
     4081 gccgaggtcg aagctcggca tgcggaccag cgagatcagg ccgtcggtca ggcacaccgc
     4141 gagcaccgtc gccagcaccc atcgcggctt cgcggcccca cgaccggcgc acaggcccat
     4201 ggcgaacagc acgtagggca gcgcgtcctc gagggcctgc gcgagcgagt tgccgcgcag
     4261 gatgccgacc atcaggctca ggccgagggc gaccagccac ggggcgagca gcgccagctc
     4321 ggcgcgggcc gccgcgctgc gcacgaagcc gccgagcccg gccagcatca ccagcccgag
     4381 gaagccgacg atggggatcg tcaggccgtc cgggaaggtc ggcagggcca gcgtcagcag
     4441 catcgccgcc gccgcgaaca gggtcgtcag caccgcccgg cacgctagca cgatgcagcg
     4501 agccggcagg catggggtcc tcgggaccca ccggtctcca ccgatcgcca ccgatcgcca
     4561 ccgattttgg ccgcggccgg ccgcgatctt tgccgctccc ggcaaggggc acatcggttc
     4621 agtgtgcagc gctcgacacg gcgttcatct cacacctcga tcatcggccg cgggaatgtc
     4681 cgcaaagccc acgagttgca gtcctgccgc tgtcaaattt tcggcttttt tgaaagccgg
     4741 cgatcggccc gcacaggccc accatcaatc tccctgcgat catgggcctt cgccgcctgg
     4801 atccagcacc gatctcccgt ggacgtggcc caacgcccgc gcaaagtggg ttccgggcct
     4861 tgcacggggg ggctcgattg catagcttgc ttgctctcgc gtcggctcgc gacgcgtgcg
     4921 ggatcttggt catgcaccgg caatggttgg actggcatcg accgctgctg cccgcggcgg
     4981 ccgcctggct catcggccag catagcgacg ggaagcgctg cgacctgcgg caggtccgtt
     5041 gcgtgctgcc gggcacccgc gccggtcgcc tgctcctgca cgcgctgctg gcccagtgcg
     5101 ccgagcaggg cctgcgcctg gtccccccgc aggtgctgac ccccggcacg atggtcgacc
     5161 agctggtccc gccgaccccg gacaccgcct cggccctcga gtgcacgctc ggctggatgc
     5221 acgccctgcg cgacgccggc atcgacgaga tcgcatcact gctgccgagc cgccccgact
     5281 cgcaggactg gccggcctgg cacgagctcg ccgggcagat cggtcgcatc gtcgaggagc
     5341 tcgccggtga gctgctgagc ctgcgagacg tcgctcgcgt cgccgggcgc atggacatgg
     5401 agcgcgaggc ccagcgctgg gccgtgctcg agcgcctcgc cgaccgctac cgcgagcgcc
     5461 tgcgtcgctg cggccgcgtc gacccgcacg agcgccgccg cgaggccgtc gcctcgctcg
     5521 tcgacgccga cgtcccggtc gaccccggct accagctcgt gctcgtcggc gtcgtcgacc
     5581 tggcccgggt ccagcgcgcc gtcgtgcgcg cctacggcga gcgagcgacc gccctgatcc
     5641 acgcccctga catgctcgcg cacggcttcg acgccgacgg ttgcatcgag cgcaccttct
     5701 ggaccggccg catcgtccac gtcccggacg agcagctgat catcgccgac cgcccgagcg
     5761 accaggccca ggccgtcgtc gagtgcctgg cgaagctcgg cggctacttc cgtcccgagc
     5821 agatctcgat cggcctcggt gacgacaagc tcggcccggc gatggcccag gccggcgagt
     5881 gggccgacct caccgtgcac gacccgcgcg gccacgtcgt cctgcgcagc cgcccgtttc
     5941 gcctgctcga cgccggcctc gagtgggtcc gcgacccccg cttcggcgcc ttcgcctcgc
     6001 tgatccgcca cccggacgtc gaggcctggg tgcgccgctg cctcggcgcg cgcaagacca
     6061 ccgccaccgc cgaggcaccc gcgaccgaag ctccggcaga gcccgcgccc gcggccgagt
     6121 caccgcccgc gctcacggtc gtccccgacg ctcccaccac cgaggccacc gcacctgtcc
     6181 aggaggacat gccgatcctc gagtggctga cggtcctcga tcgctactac accgagcatc
     6241 tccaggacac tggcggtgga ccgtggctcg gcgacgagaa gaccgtgcgt cgcatggaga
     6301 tcctcgacga catcgtgcgc aagctgttcg ccccgctgcg cggcgagcga cgcccgctga
     6361 cggagtggtt cgagcccgtt ttggaagtcc tgcgcctcgc ctacggccac atcgacgtcg
     6421 gcacgcagca aatttccgag gcccgcgtcg ccgcgacctg cctcgagatc gcccgcacgc
     6481 tcggcggtct gaccggagcc gccgccgagc tgcagccccg cgtcgacggc ccgaccgcgc
     6541 tgcgcctcgt gctcgcgcag atcgccggcc tgcggatccc cgaggacccg cgctccggcc
     6601 aggtcgagat gctcggctgg ctcgacctcc accatgaccc cgcgccggtg ctgatcctcg
     6661 ccggcttcaa cgacggcgcg atcccgcagg cagtaagcgc cgatgccttc ctgcccgatg
     6721 gtctgcgcga gcacctcggc atcctcagca acggccgccg ctacgcccgc gacatttatc
     6781 tgctggaggc catccgccac agccgcgagc gctgcacctt catcgccggg cgccaggccg
     6841 ccgacggtga gccgctgact ccgagccgcc tgttgctcgc cggtgagcag cacctgctgc
     6901 ccgcgcgcgt gctaaagctg tgcgacccgc agcacgcgct gcgccgcaac ttgccctacg
     6961 gggcgccgcc gtccggggcg acctccggct ttcgcatccc ggaggtcccc acggcacctg
     7021 taccgcccga ggcgatgacg gtcaccgagt tcggctggta cctgcgctgc ccgtatcgtt
     7081 attacctcaa gtacatccgt cgcctgcgtc aggtcgagga cagcgccgtc gagctcgacg
     7141 ccttgcagtt cggcaacctg acgcacgagg tgctccagca cttcggcgag gaccccgatc
     7201 actcgcgcag caccgacccc gtgcggatcg ccagctttct cgaagagatc ctcgtcgacc
     7261 gcgcgcgcgc cggtttcggc gcccaaccac tgcctgcgat ccggatccag ctcgcccgcc
     7321 tgcgcgagcg cctgcggacc ttcgcccgct accaggcgcg ccgctcgtcc gagggctggg
     7381 tgatccgccg ctgcgagttc gagctgcccg aggcgaccta cctcgagatc cccgaccagg
     7441 agaagatgcg gatcaagggc aagatcgatc gcatcgatca aaacgagcgc gacggctcgt
     7501 ggatgatcat cgactacaag accagcgagg ccggcaagac ccccgaccgc acccacaagg
     7561 cttcgcgctc ctcgggccgc gtgtgggacg acctccagct cccgctctat caccacctcg
     7621 ccggccagca cggcatcaac ggccccgtcc agctcgcgta catgaacctg ccgaagaagg
     7681 ccgagcagga gaccctgcac gtcgctcgct ggtccgccgc cgacctcgac gacgcgatcg
     7741 gcaccgcccg cgacatcgtc cgcaacatcc gcgccggccg cttcgagatg gccgacgact
     7801 accccgcgcg cttcgaggac gcgttcgcca acatctgcca gactcgtgtc ttcggcggcg
     7861 gcgatgacct ggagaccgag gcttgagcgc gcgccgtaac acctcggcga cccccgcggc
     7921 tgccgcctcc gacgggtcgg ccgcacccga gtcgctgtgg ctgttcagcg actcaccgat
     7981 cgcgccgccg ccggtgccgg cgcgcgtccc aaaaacttca cagcccggca gcccgcagca
     8041 gcgctagggc cctcgcaagc cccgctcctc ccgctcgagc cgtgacctgt tctccgcggt
     8101 cgaccgcgtg ttcgcccgca gcagcgaggc cgcccgcgcg gagatcgccg gcgaggcgcc
     8161 acccgccccg caggcccgcc accgcgtgcc tgccgagggc ccgcgaccgg tcccggaggc
     8221 caggccggcc caacctgtcc aaaaggacac ccgtcccgcg cccgcgcgcg gccccgccga
     8281 cgaacctgtc ccatgggaca gcgacgacgg ccagtggacc gaccacggcc ccgacatcga
     8341 cctcggcgac ctgccggatc tgccgtggtc gacccctgcg ggcgagctgg agatcaacct
     8401 catcccgggc gggtcgcgca ccgaggtgct cgcgctctta cgcgaggccg aggacgtcgc
     8461 cagcagcgcg atggtcttcg aggacgaccg caacctcgag ggtgacgcct cgctgcagca
     8521 cgtcagcatc ctcgcctccg cgggctcggg caagacctac cagctgacca accgctacct
     8581 gcagatcctc gcccgcggcg cggctccgta cgggatcctc gcgagcacct tcacgcgcgc
     8641 cgcggccggt gagatccgcg atcgaattct gcgcacgctc gccgagtcgg ccgaggtcga
     8701 cgacaagcgc gacgagctga tgcgtcgcct ccaccgcccc ggcatgtcgc ggcagttcgt
     8761 actcgacatg ttgacggcgc tggcccacaa cctccaccgc ctccagatcc gcacgctcga
     8821 cagcttcttc ggcacgatcg tccgctgctt cgcgctcgag ctcgggctcc cggccgacgc
     8881 ccgcatcatc gacgaggacg aggcgtttcg tctgcgccgc gccgcgatcc agcgcatgct
     8941 cgaggagggc gacgaggccg cgatcatcga gctgctcgcc tcgctgaccg agggctcctc
     9001 cgagcgcgcc gtcaccgaca cgatcgacaa caccgtcagc tctctctacg acctctaccg
     9061 cgaggcccgc tacaccgatg cctggcgggc gatcgccccc ggcgagtgcc tgaccgaggt
     9121 cgccctcgac gccgcgatcg ccgacctgcg cgccgccgtc ccgtccggcc ccaagcgcac
     9181 gatcgactgc cacgccaagg actgcgagcg cgccgagcag tgcaagaccg gcagctccga
     9241 cgactggatg gagttcttcg gccagggcct cgccgccaag atcgcggacg gcagctgcgt
     9301 ctactacgcc aagccgatcg acgcccgcac cgtcgccgcc tacgagcgcc tcttaaagca
     9361 tgcgaagggc gtgtaccggc ggcgcgtgat cgaccagacc cgcgccaccc acgagctgct
     9421 gctgcgattc gacgactgct accagcgcgt caagcatcaa cacaaggcgc tgaccttcgc
     9481 cgacctgacc gtcgcgctcg cgcaggccga cctcagcggc aagctcgacg acatctgtta
     9541 tcgcatcgac gcccgcctgc gccacgtgtt gctcgacgag atgcaggaca ccagcatcca
     9601 gcagtggaac gcgatgcgcc cgatcgtcga cgagacggtc agctacaccc cggaggagcg
     9661 cagcttcttc tgcgtcggcg acgtcaagca gtcgatctac ggctggcgca acgcctgccc
     9721 ggagatcctc agtcgcatcg accagctttt gggcgccggc gccggcatcg tcaagcagaa
     9781 cctcgctacc agctatcgca gcgcgccggc cgtcatcgag gtcgtcaaca agatcttcac
     9841 cgcgctggcc aacaaccccg cgctgatgcg ctaccccgac gccgcgcagc gctgggacga
     9901 ggagttccag acccacacca cgcagcgcag cgcgctcgcc ggctacgtcc gcctgcgcac
     9961 cgtcgcccgc tgcgaggacc aggccaagcg ccagaacttg cgcttgcaag aggccgcgca
    10021 cctcgtccac gagctgtatc accgccaccc gggcaagacg atcggtgtcc tgctgcgcac
    10081 caacggcgcg gtcggtcgcg tgctctacga gctcggcccg acgcggctca aggtcccggc
    10141 gaccggccgc ggcggcggcc ctttgacgga cgcgccggcc gtcaacgcga tcctcgatct
    10201 gctgcgcctc gccgaccacc ccgaccacag cgtcgccgcc ttcaacgtgc tcagctcccc
    10261 gctcggtccg gtcgtcggtc tcaccgagga cggccctggc ccgcgccacc gcgccgccgc
    10321 ccgcgtgcgc cgcatggtcg ccagcgacgg cctttcgcgc accttgcagc cgtgggtcaa
    10381 ggcgatcgcc ggtcaggtcg acgagcgcca gtatcgccgc tgcatgcagc tgatccagct
    10441 cgcgcaggcg tgggaccagc gcgccagttt gcgctgcgac gacttcgtcg ccatggtcga
    10501 gacgcgcgcc gtcgccgacc cctccgcctc cgccgtccag gtgatgacga tccaccaggc
    10561 caagggcctc gagttcgaca tcgtcatcct ccccgagctc gaggccgagc tcgcctcgac
    10621 gcgcaccctc aaggtcgtct acgagcgcaa cggcgacgcc ggcccgatct cgaagatcgc
    10681 acgacatgtc tcgaaggaca tgtggtcgat gttcgacgac ctgcggccga tgttcgagca
    10741 gcacatcggc cgcctcgcgc acgagagcct gtgcctgctc tacgtcgccg tcacccgcgc
    10801 gcgcgagggc ctgttcatgc tgatcgaccc gcccggcgag agcagccgca ccgcgcccgc
    10861 gcgcatgtcc tcgctcgtgc tgcacgccct gcacgacgga ccggccgacc ccgactccgt
    10921 cgtgttcgag tggggcgacc cgcagtggct cgcgccgcac gcccccgtca ccgccgcgcc
    10981 cgtcgacccg tccgcgccgc acctcgagtc cccgcaggcg cccctgacct tcgcgccgac
    11041 ccagggcccg ctgctgcgca gcaccggcgc catggcgagc gacctcgccg gccgcaccgt
    11101 cggccaggtc ctcgtgctca acgacggccc gacctccgcc tggggcgggg ccgtgcgcgc
    11161 cctgttcgcc gaggtcacct ggctcgagga tttcgtggaa gaccgccgcg cgctgcttgc
    11221 cgcggtgcaa aaggccgccc cgaaccgcga cgaggagtac gcccagaccg tcgtgctcga
    11281 gttcctgcgc atgctcaccg ccgcgaccgt gcgtcgggcg ctcagccgcg gcggcgcccc
    11341 ggcgaacgcc ttgcaggtcg agcgccaggt gcccttcgcc cgcctcgagg ccggcgccct
    11401 ccagaccggc gagatcccgc gcgtcgtgct cgagctggac ccgaacctgc ccgaagggac
    11461 agagggccac gtccgccgcg cgaccgtgct cggcttctgc ctcgacaacg tcgacaccca
    11521 ggagctcgcg gccgagcgcg ccctctccta ccgcgagccg atgtccgcct ggcgaaacgc
    11581 gaccgccgag cagtggggca tcgaacgcga ccaggtcgcc ggcgtgctgc tgttcgtcgt
    11641 cgccggcatc gccgtcccga tgacctgatc gccatgggct ccgacaccct cgccgtccgc
    11701 atcgccggtg tccacgacac caccctcgag ttctgggccg ccgagctgcc ccagggcccc
    11761 gatggctcgt tcgtcagtgt gatcggttgg gatcaccccg cgctcgcgct gatgctgatc
    11821 gccggctccg cgaaccatcg aaatgcgctc gcgcaggcgc tcgagggcgt ccgctactac
    11881 gagcgcgagt ggctcgccga gcacacggac gagttcgtga cctcgatgac gctcatcgcc
    11941 accgcgcgcg ccgagtggca caccaacgag tggatcgccg gcacgatcca gcaggccggg
    12001 tcgagcgaga cctcccgggc gctggccctc cagatcgcgc cgcgggccct gtatcgcgtc
    12061 accgtccgcg accccgcctg gctcgcacac ctgcgaccag gccaggagtt cggcagcacc
    12121 gcctactcgc cgttcccgct ggtgccccgc gacagcaccg ggaccatgac gacgccgggc
    12181 gcgtgggatc cggtgctcgc ggtcatgcgt gccccgatcg acgagcgccg gatccacatg
    12241 taccgcaacg ggctctcgcc tggcagctgg tgtatcgatt acgagtccga ccgcaccgca
    12301 ccgtcgaacc gcgtgcgcga gctgttcctg cgcctcggcg aggcccagtt cgtgtacttc
    12361 gacgcgaccg cgccgcgctt caaccgcgcc aagttcccgt tcgccgagcg caagcagctg
    12421 ctcgccccgg cctacaagat gtgggaatcc ggggccgcgg ccgggccgcg gggcttccac
    12481 ctgaagtcga gggtctcgca gcgccatgaa gacggcgtcg cccgcatgca gctcgacgac
    12541 ctctgtcccg gcaagcgcca cgcctggctc gaggtcgagg gcgagccgcg cgtgcgcttc
    12601 ggcgactgga gtagcgtcga ccccagcagc ccgtccgtcg acgtcgacga ctacctgcgc
    12661 gacgtcgccg agcgcgtgcg tctcgacctc gctacaccgt gatcgtcttg ccgagcggca
    12721 gctcgcgcac gcgctgcccc gtgagcgcga acagggcgtt ggcgatcgcc ggggccaccg
    12781 gcggcacgcc aggctcgccg atcccaccgg gcgccttgtc actctcgacg atctcgacgt
    12841 ggatcttgcg cggtgcctcg ccgatgcggg tcacgcggaa gtcatggaag ttcgactgct
    12901 cgaccgcccc gtctttcaat gagatcgcgc tgtgcagcgc gaggctcatg ccgaagatca
    12961 ccgcgccctc catctgcgag atgacacggt ccttgttgat gacgatcccc gcgtcgatca
    13021 ccagccacac ctcgtcgacc accagcttgc cgcccgcccc ctgcaccacc gaggcgacca
    13081 ccgccgcata actcaaaaag ctgcggtgcg ccgcgagccc gagcgccttg ccgtcctgct
    13141 tgcgcgtgtc ccagcccgaa agcgccgtca cccgctcgac gacgtgccgc agccgtccgg
    13201 cgtcgaccgg atgtttttca aggggctgcc cgtaattctt caaactctcg acgccgagct
    13261 ctttgatcgt catgtagcgc ggcggcccga gcacctcgag ccaggtcgtg cgcgggtcct
    13321 cgccacgggc ctgggcgatc tcgtcgatga agctgccgac cgcgaacgcg tgaaagatgt
    13381 tgtagaccga ccgcagccag ccgacacgca cgtgcgcctt cgcctcgcag gactccgcgg
    13441 tcacgttcgg ggccgccagc gcgaggtcca gcacgccctg ctgcaggtcg ttgagctccg
    13501 gccggtcgat gtccttgaag agcgagccga tcggcgggaa cgcggtgcgg tgccgccacg
    13561 cgacgagctt gccaccggca tcgagccccg cggtgagccg ctgcgcgctg accgtattaa
    13621 aatagtcgtg gcggacgtcg tcctcgcgcg tccattgcac acgcaccggc acgcccgcct
    13681 gcttcgccag caacgccgcc tcggcgacga agtccggctt cgacttgcgc ccgaacccgc
    13741 cgccgaggaa ggtcacgtgc acggtgacca tgtcctcggt cgtctcgagc acgcgcgcga
    13801 cctccttgcg ggcggactgc gggtgttgcg tcggcgccca cacctcgcag cgttccccct
    13861 cgaagcgggc gaccgcgacg ggcggctcca tcgacacgtg ggcgaggtgc ggcacgaagt
    13921 agtccgcctc aatcaccttc gccgcgctcg ccagcgccgc ctcggcgtcg ccgatctttc
    13981 gcaatacctc gccgggctcg ccgagcgcgg cgatcaacgc ctgacgatat ccgctcgagc
    14041 catggctcgc gttgtcgccg tgcgcccacg tgatgtcgag cgcggcccgc ccgcgcatcg
    14101 ccgcccaggt gttgtccgcc agcaccgcga gcccaccgag cggcttgaac gcccacggat
    14161 gctcggccgc ggggatctcg atcacctgtc ggaccccggg caccgcgagc gtccgggtcg
    14221 cgtcgtagcg cgccactttt cccccgacca ccggaggccg ggcgatcacc gccgtcagca
    14281 tgctttctag acgaacgtcg gcgccgaaga tcgccgcgcc ggtcacgtag gccggcgcgt
    14341 ctatcaaggg caactggccc gcgatcgtgc gcagctcctc gcgcggccgc agcaccacct
    14401 cgtgcggctc gggcaccttc agcttcgccg cctcggccgc gagctcgccg aagccgagcg
    14461 tgcgcccgct cttctcgtgg atcacccggt gctcacgcgc cgtacacgtg cccgcgtcga
    14521 ccttccagcg cctcgccgcc gccgcgatca gcatcgtgcg cggcgtggcc ccggcgcggc
    14581 gcatcgcctc gtaaattccg cggatgctgt tcgacccgtc ggtgttctga tcgccgtagg
    14641 ccgggtctcc gtcggcctgc aagatcttca cgcgggccat gtcggcgccg agctcgtcgg
    14701 cgatcaacac cggcagggtg ctccggatcc cctggcccat ctccgagcgc tggcagacga
    14761 tcgtcaccgc cccgtcgagc ccgacgtgca cgaacacgtt cgggttgagc cccggcgcct
    14821 tcgcctccac ggtcggcgag ctggtcgtcg gcggcttggg cggcccggcc tcgggctcgt
    14881 ccgcccacgt ctcaccactc ttgaagaatc cgagcgcgag cccaccggcg ccgatgccga
    14941 gccccgcgag aaaactacgc cgtgcgacca gcatgcgtca ctctcctgcc acgcccgcgg
    15001 cccgcttcac ggcggcgcgg atgcgcgtgt aggtgccgca gcggcacagg ttgcccgcga
    15061 gggagcgatc gatctccgcg tcgctcggct gcggtgtgac ggccaacagg gccgccgccg
    15121 tcatgatctg cccggcctga cagaacccgc actgcggcac accgagctcg caccaggcct
    15181 tttgcagcgg gtgatctccc gcgggcgaga ggccctcgat cgtcgtcacc gtgcggccct
    15241 cggcgcggcc gaccggcgtc acgcacgatc gaaccacctg cccgtcgagg tgcaccgtgc
    15301 acgcgccgca cagcgcctga ccgcagccgt acttggtgcc ggtcagcccg agcacgtcgc
    15361 gcagcgccca cagcagcggc atctccgggt cgacctcgag ctcgcgctcg acgccattga
    15421 tgcggatctt catggcctcg cctcctcggc ggggcactcg gccccggtct cgacccacgc
    15481 ggcga
//
LOCUS       DASWNJ010000021         2994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_353778, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000021 DASWNJ010000000
VERSION     DASWNJ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353778"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VGB85_00480"
     CDS             <1..924
                     /locus_tag="VGB85_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132509.1"
                     /translation="PRPVRDFLPDFDYHDLEATLRAALRYRPEDRTPTMSELRDGLDL
                     ARECLLAQREEIGAMTSGPKVSAPAEREQVAVDTAAKTPPHAEGGQAEASYEPTREPA
                     PREQVKAVQTPLVPSRPRGRLAGVALAAGLGLAAGIGGTLAMRPGEPVQFAVHQEPVA
                     ARMELERARAEARADRCEAEAARQTPRVELAPPSPSSAPSQPPSPSPDRPAARVLAAS
                     RAVSADRPASTKSPNAMARLAPEIRACARKGGIPEGPITVQVRRQGGVIDSVKLLKFS
                     KDHPSVPCIDKAIRKADPPGNRPLEDFTFSK"
     gene            933..1784
                     /locus_tag="VGB85_00485"
     CDS             933..1784
                     /locus_tag="VGB85_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132510.1"
                     /translation="MLAIIFLPLLLASPEPPSAEALAAEGEAYLKAAATAERPLDVLL
                     SAHTSFDSAYLDGDDPGYLCRALDVTGRALQLPALADEQERKFWEETRREDLERLQRD
                     AAEKRRVNCRYDAAGKPAQPRVALLAADDPPAAPVLPAPLATPPATARRTLNGPSRRQ
                     VHRDRAHTAMGAVLTGAGIGMLGILAGILELERQRAVEVRGIIEAAKAEQRGYTPAEE
                     RRYLDLRDDLIRGRDAAIGVGVAGLVSLGTGVALLATRKKARSRSYALAPYGGPQGVG
                     AVLRLRF"
     gene            1821..>2994
                     /locus_tag="VGB85_00490"
     CDS             1821..>2994
                     /locus_tag="VGB85_00490"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY0132511.1"
                     /translation="MKSLPAALLLGGCLQPLLDEGDGLVSLTATEADSSSTSSMGEPP
                     TPTTSAGSGVQTVTGGEETTGPSTTSSGDDPGSSSGDAVNQPPTIELFTVDTDYIDAA
                     GPLTLTLGASDDHAVVKVRLYLDGVKLADLTPADFPYTFDVLSAKFNGDQRIFKVVVE
                     DAEGLAAEREADPVKVWLPASGVERCIFTDPGEGSVISYVSAIKFTPEAIIAVGTRDT
                     GAGPMLTVWKLDPDTCDLRPGWPKSMTNWSTHDDYKKAVSIGAAVDIDEDGNIVVAGN
                     FWVGNKPQGYVVLLTESGSRLWEKAGQPGDEITSVAAATGQFKDRVFVGGSLRTSENP
                     VRTDGAVWVYQANGETAFVSPPNILAAPFAPGEQLDDGNKRSEWVRALVIHPSTGNA"
BASE COUNT          478 a         1066 c         1049 g          401 t
ORIGIN      
        1 ccgcggccgg tccgagactt cctcccggac ttcgactacc acgacctcga ggccaccctc
       61 cgcgccgcgc tccgctaccg acccgaggat cggacgccga caatgtccga gctgcgcgac
      121 ggtctcgatc tggcccgcga gtgcttgctt gctcagcgcg aggagatcgg ggcaatgacc
      181 agcggcccga aggtctcggc gcccgccgag cgcgagcagg tcgcggtaga caccgcggcg
      241 aagaccccgc cgcacgccga gggtgggcag gccgaggcgt cctacgagcc aacgcgtgag
      301 cctgccccgc gtgagcaggt gaaggcggtc cagactccgc tcgtcccttc ccggcctcgt
      361 gggcggcttg ctggggtcgc gctcgcggcg gggttggggc tcgcggccgg catcggcggg
      421 acgctcgcca tgcggccggg tgagcctgtg cagttcgccg tccaccagga gcccgtggcg
      481 gcccggatgg agctggagcg ggccagggca gaagcgcgtg cggaccgctg cgaagccgag
      541 gcggcgaggc agacgccccg tgtcgagttg gccccgccgt cgccaagttc ggcgccaagt
      601 cagcccccct cgccgtcccc cgaccgaccc gcggcgcgcg tgctggcggc gagccgtgcc
      661 gtgagtgccg accgtccggc gtcgacaaaa tctccgaacg ccatggcccg gctcgcgccc
      721 gagatccgcg cctgcgcgcg gaagggtggc atccccgagg ggccgatcac ggtgcaggtc
      781 cgccgccaag gcggcgtgat cgactccgtg aagctgctca aattttcgaa ggaccacccg
      841 tccgtcccgt gcatcgacaa ggcgatccgc aaagccgacc ccccgggcaa ccgcccgctc
      901 gaggacttca cgttttccaa gtaggagctg cgatgctggc gatcattttc ctgccccttc
      961 tcctcgcatc accagagccc ccgagcgccg aagctcttgc cgccgaaggc gaggcgtacc
     1021 tcaaggccgc ggcgacggcc gagcgaccgc tggacgtgct ccttagcgcc cacacgagct
     1081 ttgactcggc ctacctcgat ggcgatgacc cgggctacct gtgccgggcg ctggacgtga
     1141 ccgggcgggc cctgcaactg ccggcgctcg ccgacgagca ggagcggaaa ttttgggaag
     1201 agactcggcg tgaagatctc gagcgtctgc agcgggacgc ggcagaaaag aggcgagtca
     1261 actgccgcta cgatgcggcg ggcaagccgg cacagccgcg cgtcgccctg ctcgcggcgg
     1321 atgatccccc ggcggcgccg gtactgccag ccccgctcgc caccccgccg gcgaccgcac
     1381 gccgcacgct caacgggcca agtcggaggc aggtccatcg ggaccgcgcg cacacggcga
     1441 tgggcgccgt ccttaccggc gctggcatcg gcatgctggg catcctcgcc gggatcctcg
     1501 aactcgagcg tcaacgtgcc gtcgaggtcc gcgggatcat cgaggcggcc aaggcggagc
     1561 agcgcggcta cacgccggcc gaggagcgcc gctacctcga cctgcgcgat gacctgatcc
     1621 gcggccgtga cgcggcgatc ggcgtcggcg tggccggact ggtgagccta ggcaccggcg
     1681 tcgcgttgct ggcgacccgg aagaaggcaa ggtcaaggtc gtacgcgctc gcgccgtacg
     1741 gcggcccgca aggcgtgggc gcggtgctgc ggctgcggtt ctgaccgcga ggaagcgtga
     1801 cgatgcacag gttcaccttg atgaagtccc tccccgccgc cctcctgctg ggcggctgcc
     1861 tccagcctct actggacgag ggcgacggtc tggtctccct caccgccacc gaggcggaca
     1921 gcagctcgac gtcgtccatg ggcgagccgc ccacgccgac gacctcggcg ggctcgggcg
     1981 tgcagacggt gacgggcggc gaggagacca ccgggccgag cacgaccagc tcgggcgacg
     2041 acccggggtc gagctcgggc gacgcggtca accaaccgcc gacgatcgag ctgttcacgg
     2101 tcgacaccga ttacatcgac gcagccggac ccctcacgct cacgctcggg gccagcgacg
     2161 accacgccgt cgtcaaggtc cgcctctacc tcgacggggt gaagctcgcg gacctgacgc
     2221 ccgccgactt cccgtacacc ttcgacgtgc tgtcggcgaa gttcaacggc gaccagcgga
     2281 tcttcaaggt cgtggtcgag gacgcggagg gcctcgccgc ggagcgtgaa gccgacccgg
     2341 tcaaggtttg gctgccggcg tccggcgtcg agaggtgcat cttcacggac ccgggtgagg
     2401 gctccgtcat cagctacgtg tcggccatca agttcacgcc cgaggcgatt atcgcggtcg
     2461 gcacgcgcga caccggcgcg gggcccatgc tgaccgtgtg gaagctcgac ccggacacct
     2521 gcgatctgcg tccgggctgg ccgaagtcca tgacgaactg gagcacccac gacgactaca
     2581 agaaggcggt gtcgatcggc gccgcggtcg acatcgacga ggacgggaac atcgtcgtcg
     2641 ccggcaactt ctgggtcggc aacaagccgc agggttacgt ggtgctgctc accgagtccg
     2701 gctcgcgcct gtgggaaaag gcaggccagc ccggcgatga gatcaccagc gtcgccgccg
     2761 cgaccggaca gttcaaggac cgcgtgttcg tcggcggctc gctgcgcacg agcgagaacc
     2821 cggtgcgcac agacggggcg gtctgggtct accaggccaa cggcgagacg gcattcgtgt
     2881 cgccgccgaa catcctcgcg gctccgttcg ccccaggcga gcagctcgac gatggtaaca
     2941 agcgcagcga gtgggtccgg gccctcgtga tccacccaag caccggcaac gcct
//
LOCUS       DASWNJ010000022         2541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_459887, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000022 DASWNJ010000000
VERSION     DASWNJ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459887"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VGB85_00495"
     CDS             complement(<1..388)
                     /locus_tag="VGB85_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0132512.1"
                     /translation="MTRIAFWDVSGGHGTPTLVYHLAHMFADQGRRTLLLDFDPTSRL
                     TAMCVSEERLVELWQHGSDKTIAGFIDGLLTATKDLPQPKLEELRPGLDLLPGDLSFA
                     IFEGAFARAWASGEDPESIAVLSALDR"
     gene            complement(385..1581)
                     /locus_tag="VGB85_00500"
     CDS             complement(385..1581)
                     /locus_tag="VGB85_00500"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0132513.1"
                     /translation="MLDAQEIARLAGDLESFHVERKASFKPAKSDIEEAICAFANDLP
                     GAGRAGVILIGVTDKTGQPTGLAVSDELLREITDIRSSGNILPFPVMSVYKATLGSHE
                     IVVIEVHPSHDPPVRLRGRVCVRVGPRKGTATRDEERVLNERRRGWDGPFDQRPIGGA
                     SLDELELTVFEREFLPSAVTPEVLRENGRSRAEQLSALHLASPDGVPNVAGMLILGRD
                     PTSYIPGAYVQFVRVDGPELTDPILDRKELMGPLPEVLRRLDEITDAHIRVATTVAGS
                     TVERRAPDYPIAAMQQLLRNAVMHRNYETSNAPVQWYWFSDRIEIHNPGGLYGRATPQ
                     NFGKPGGNDYRNPTIAGALYLLGFVQRFGMGILMARKACSDNGNPPPEFIFEATNFAV
                     IVRTRA"
     gene            complement(1644..2333)
                     /locus_tag="VGB85_00505"
     CDS             complement(1644..2333)
                     /locus_tag="VGB85_00505"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY0132514.1"
                     /translation="MPRHGVLTDELLAAALDGERRPDLAARSPRFFHRGSHERLTRAH
                     PASPYILYVPAIAACLWLASSLRVPSIVALLAAGWLVWTLVEYWLHRGFFHMRADSPA
                     RRVTSYIVHRHHHAAPQDTDRLVATPLYSLGLFLLLLAPYSLAGPAARWALMAGTMLG
                     YLGYEAAHWRVHHVRPGRSGRLTRALRRHHLRHHFGDDAHNFGISSPLWDHIFRTWRT
                     GPVREDRNHDA"
     gene            complement(2343..>2541)
                     /locus_tag="VGB85_00510"
     CDS             complement(2343..>2541)
                     /locus_tag="VGB85_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEY0132515.1"
                     /translation="HALRLPARAIIMLGSETHGLSPAIAKLADDSVRIPGTGRLDSLN
                     VACAASVLLSEYWRTHPGTTA"
BASE COUNT          379 a          763 c         1014 g          385 t
ORIGIN      
        1 ctcggtcgag ggcggacaac acggcgatcg actcaggatc ttcgccactt gcccacgcgc
       61 gggcaaacgc cccctcgaag atggcgaacg acaggtcgcc gggcaacaga tcgagtcctg
      121 gacgcagttc ctcgagcttc ggctgcggga ggtccttcgt ggccgtcagc aggccgtcaa
      181 taaacccggc gattgtcttg tcgctaccgt gttgccacag ctcgacgaga cgctcttccg
      241 acacgcacat tgcggtcagc cgggaggtcg ggtcgaagtc gaggagtagt gtccgtcggc
      301 cctggtccgc gaacatgtgt gcgaggtgat agacgagcgt aggcgtgccg tgcccgccgc
      361 tgacgtccca gaacgcgatg cgcgtcatgc ccgggtcctc acgatgacgg cgaagttcgt
      421 ggcctcgaag atgaattcgg ggggagggtt gccgttgtcg ctgcaggcct tgcgggccat
      481 caggatgccc atgccgaagc gctgcacgaa gccgagcagg tacagggcgc cggcgatcgt
      541 cgggttgcgg tagtcgttgc cgccaggctt accgaaattt tgtggagtgg cccggccgta
      601 aaggccgccc gggttgtgga tctcgatgcg gtcggagaac cagtaccact ggaccggcgc
      661 gttgctggtc tcgtagttgc gatgcatgac cgcgttgcgc agcagctgct gcatcgcggc
      721 gatcgggtag tcgggcgcgc ggcgctcgac ggtgctgccg gcgacggtcg tcgccacgcg
      781 gatgtgggcg tcggtgatct cgtcgaggcg gcgcaggacc tcgggcagcg ggcccatcag
      841 ctccttgcgg tcgaggatcg ggtcagtcag ctcggggccg tcgacgcgca cgaactggac
      901 gtaggcgccg gggatgtagg aggtagggtc gcgcccgagg atcaacatgc cggcgacgtt
      961 gggtacgccg tcgggggagg cgaggtgcag cgccgacagc tgctcggcgc gcgagcgtcc
     1021 gttctctcgc aggacctcgg gcgtgacggc gctgggcagg aactcgcgct cgaagaccgt
     1081 cagctcgagc tcgtcgaggc tggcgccgcc gatcggccgc tggtcgaagg ggccgtccca
     1141 gccgcggcgt cgctcgttga ggactcgctc ctcgtcgcgg gtcgcggtcc ccttgcgcgg
     1201 gccgacgcgg acacagacgc ggccgcgcag gcgcaccggc gggtcgtgcg agggatggac
     1261 ctcgatcacg acgatctcgt gcgagccgag cgtggccttg tagacgctca tcaccgggaa
     1321 cggcaggatg ttgccgctgc tgcggatgtc ggtgatctcg cgcagcagct cgtcgctgac
     1381 ggcgagaccg gtgggctggc cggtcttatc ggtgacgccg atcaggatca cgccggctcg
     1441 tccggcgccc ggcaggtcgt tggcgaacgc gcagatcgcc tcctcgatgt cgctcttggc
     1501 cggcttgaag ctggccttgc gctcgacgtg aaagctctcg aggtcaccgg ccagcctggc
     1561 gatctcctgc gcatcaagca tgacgagccc cggccatgat gtcccgccgg caggttatcg
     1621 ggcgggagtg ggacacgcaa gggtcaggca tcgtggttcc tgtcctcgcg gacaggtcct
     1681 gtgcgccagg tgcggaagat gtggtcccac agcgggctgc tgatgccgaa gttgtgggcg
     1741 tcgtcgccga agtggtggcg caggtggtgg cgtcgcagcg cgcgggtcag gcggccggag
     1801 cggccggggc ggacgtggtg gacgcgccag tgggcggcct cgtagccgag gtagccgagc
     1861 atggttccgg ccatcagggc ccagcgcgct gcgggcccgg cgaggctgta gggcgcgagc
     1921 agcaggagga acaggccgag gctgtagagg ggggtggcga ccaggcggtc ggtgtcctgc
     1981 ggggcggcgt ggtggtggcg atggacgatg tacgaggtga cgcggcgggc gggcgagtcg
     2041 gcgcgcatgt ggaagaagcc gcgatgcagc cagtactcga cgagggtcca cacgagccag
     2101 ccggcggcga gcagcgcgac gatgctgggg acgcgcagcg aggacgcgag ccacagacac
     2161 gccgcgatcg ccgggacgta gaggatgtag ggcgaggccg gatgcgcgcg ggtcaggcgc
     2221 tcgtgggacc cgcggtgaaa gaagcgcggg gagcgggcgg cgaggtcggg acggcgctca
     2281 ccgtcgaggg cggcggccag cagctcgtcg gtgaggaccc cgtggcgcgg catggcgatc
     2341 cgtcaggccg tcgtgccggg gtgggtgcgc cagtactcgc tcagcagcac ggaggcggcg
     2401 caggcgacgt tgaggctgtc gaggcgcccg gtgccgggga tccggaccga gtcgtcggcg
     2461 agtttggcga tcgcaggcga gaggccgtgg gtctccgagc cgagcatgat gatcgcgcgg
     2521 gccggcaggc gcagcgcgtg c
//
LOCUS       DASWNJ010000023         2403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_283208, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000023 DASWNJ010000000
VERSION     DASWNJ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283208"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VGB85_00515"
     CDS             complement(<1..307)
                     /locus_tag="VGB85_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132516.1"
                     /translation="MRITRRSWLHGSFLALGAATLLARQGRVRAAPGAPPRRLVLVFN
                     SGGWDPTYALDPKPGVGGVDAPAGKVASLGGLDVLTDPSRPNVGAFFTAHAGSCAIVR
                     "
     gene            complement(317..2014)
                     /locus_tag="VGB85_00520"
     CDS             complement(317..2014)
                     /locus_tag="VGB85_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132517.1"
                     /translation="MSVLLALAACTGDDSVAETTATGSTGAGTTGDTGDVTPTGGEPQ
                     EEPEMLPPRARLLRISMALRGLRPTEDELAAVEADPDALPGIVDAYLASPEFGAIVRD
                     LHNDALLVLADFGVPPAGFLAKDTLAGLDVYAANRAIMEAPLRLIEHVVAGDRPYGEI
                     VTADYTLADEAVAGAWGLAHAGTGWQVVDPPSDLRGAGILADSWLFTRHGSTVSNAGR
                     GRANAVSRALLCFDFLDRDIEIDASVNLADPNAVAKAVQDNETCVACHQQLDPLATFF
                     EGYKLNFVPLLVPYPHDQYAQGFFTGFLKVDMRPPRYFGYPGESLADLGAMIARDPRF
                     SLCAARRAYAYFHQVDLAEVPFDAADRLQSALLAGEMRYKAMIRALVLDPGFALASGP
                     QGRRRSRPGQLATLIADLTGFRWRTDLTPYGAGKIDLMDDSMIGYHVLAGGIDAIFVQ
                     KPSYTYNATTSLTLRGLARQAASAVVEADFAEADASKRRLFTQVSALDSHPTTLRPQL
                     VVLHRRFYGDLLAEDSPEIDESLALFLAALKASPGDIKRAWKTTLLALLQDVRIAFY"
BASE COUNT          328 a          823 c          898 g          354 t
ORIGIN      
        1 cgcggacgat cgcgcagctg cccgcgtgag ccgtgaagaa cgcgccgacg ttcgggcgcg
       61 atgggtcagt cagcacgtcg aggccgccga gcgacgctac tttgccggcc ggcgcatcga
      121 cgccgccgac cccgggcttg gggtcgagcg cgtaggtggg gtcccagccg ccgctgttga
      181 acaccagcac gaggcggcgc gggggcgcgc cgggtgcggc gcgcacacgg ccctgccgcg
      241 ccagcaaggt ggcggcgccg agggcgagga agctgccgtg taaccacgaa cgtcgggtga
      301 tccgcatggt gaggtcctag tagaaggcga tccgcacgtc ctgcaggagc gcgagcaggg
      361 tggtcttcca ggcgcgcttg atgtcgccgg ggctggcctt gagcgcggcc aggaacagcg
      421 cgaggctctc gtcgatctcc ggcgaatcct ccgcgagcag gtcgccgtag aagcggcggt
      481 gcagcacgac cagctgcggg cgcagcgtgg tcgggtggct gtcgagggcg gacacctgcg
      541 tgaacagccg ccgcttgctc gcgtcggcct cggcgaagtc ggcctcgacg acggcgctgg
      601 cggcctgccg ggccagcccg cgcagcgtca ggctggtcgt cgcgttgtac gtgtacgacg
      661 gcttttgcac gaagatcgcg tcgatgcccc cggcgagcac gtggtagccg atcatgctgt
      721 cgtccatcag gtcgatcttg ccggcgccgt agggcgtgag gtcggtgcgc cagcgaaagc
      781 cggtcaggtc ggcgatcagc gtcgcgagct gacctggacg gctgcgacgg cgaccctgcg
      841 gtccgctcgc cagcgcgaag ccggggtcga gcaccagcgc gcggatcatc gccttgtagc
      901 gcatctcgcc ggccagcagc gcgctctgca ggcggtccgc cgcgtcgaag ggcacctccg
      961 cgaggtcgac ctgatggaag taggcgtagg cgcgccgcgc cgcgcacagc gagaagcgcg
     1021 ggtcgcgggc gatcatcgcc ccgaggtccg cgaggctctc gcccgggtag ccgaagtacc
     1081 gcggcgggcg catgtcgacc ttgaggaagc cggtgaagaa gccctgcgcg tactggtcgt
     1141 gcgggtaggg gaccagcagc ggcacgaagt tgagcttgta gccctcgaag aacgtggcga
     1201 gcgggtcgag ctgctgatga caggcgacgc aggtctcgtt gtcctgcacg gccttggcga
     1261 ccgcgttggg gtccgcgagg ttgacgctgg cgtcgatctc gatgtcgcgg tcgaggaagt
     1321 cgaagcacag cagcgcgcgc gacacggcgt tggcccgccc gcgtccggcg ttgctgacgg
     1381 tcgagccgtg ccgcgtgaac agccacgagt ccgcgaggat ccccgctccg cgaagatcgc
     1441 tcggcggatc gacgacctgc cagcccgtgc ccgcgtgcgc gaggccccac gcgccggcga
     1501 cggcctcgtc ggcgagcgtg tagtcggcgg tgacgatctc gccgtacggc cgatcaccgg
     1561 ctaccacgtg ctcgatcagc cgcagcggcg cctccatgat cgcccggttg gccgcgtaga
     1621 cgtcgaggcc ggcgagcgtg tccttggcga ggaagccggc cggcggcacg ccgaagtccg
     1681 cgagcaccag cagcgcgtcg ttgtgcaggt ctcggacgat cgcgccgaat tcgggcgagg
     1741 cgaggtacgc gtcgacgatg ccgggcagcg cgtccgggtc ggcctcgacc gcggcgagct
     1801 cgtcctccgt gggtcgcagg ccccgcagcg ccatcgagat ccgcagcagg cgggcccgcg
     1861 gtggcaacat ctcgggctcc tcctgcggct cgccgccggt cggagtcacg tctccggtgt
     1921 cgccggtcgt accggcgccc gtgctgccgg tcgcggtcgt ctccgcgacg ctgtcgtcac
     1981 cggtgcaggc ggcgagcgcg aggagcacgc tcacgagcca tcgacgagtg tctgcttcga
     2041 tcacgcctgg catccttcaa gcctcgttcc acggcaatac cctttgtcat cacgcgcgcc
     2101 cggccgccgc gggctgcggt gtggcgaacc gcgacgtggt attttgccgc actcggcggg
     2161 ttgggcgcga ggtcgttcgc acgccgccgc gtgtgtgctc acgcgtgcgc gacagcaggg
     2221 catcgatctg gtaaccttgc gcgctcactg tcgttttccc gtcatgacgc tcgtccagct
     2281 ccgccgcccg cggccgccgc tcgcggccga gcccgcacga tcgatcgcgt ccggcccgcc
     2341 ggacacggcg ggctcgctcg tcgatccgca ggcgcggcgc gtgaagtacc tgcgggtctc
     2401 gct
//
LOCUS       DASWNJ010000024         3445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_459893, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000024 DASWNJ010000000
VERSION     DASWNJ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459893"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2637)
                     /locus_tag="VGB85_00525"
     CDS             complement(<1..2637)
                     /locus_tag="VGB85_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0132518.1"
                     /translation="MTLSELCIRRPIFALMMIVACVTFGVLSFGKLGVDQYPDVEIPV
                     VTVTTTLRGASPEEIETQVTKFIEDAVSTAEGIDELRSTSLEGVSIVTVNFLLDRDRD
                     QATQDVRDKVAAAVRDLPEGSDPPVVTRFDTTAVPVLTLAVSSRARDLREVTELARKQ
                     VLEPLQGVAGVGQILLMGGRKRAFNVHLDADALTAQGLSIQQVRQAIEQQNLELPGGR
                     LESPGREDVLRTMARVEDAAELGRIVVAERQGAPVTLAMVAEVEDSAEDPRSLSRLDG
                     ENAVALVIQKQAGTNTVEVVDAIFKRVQALERALPADITVAPIRDASRFIRRSIHEVE
                     LHLVLGALLASISVLVFMGSLRSTFIAALAIPCSIITTFAVLHALDYTLNNFTLLALT
                     LAVGIVIDDAIVVLETVYRRVEHGEAPFEAAIRGTREITLAVVATTVSLIVIFLPTAF
                     MAGRVGKFWRSFGITTAFAIAVSLFVALTLTPMLCSRLLKRPRPRKSDRPGAVARFNA
                     VLDNTYAAMVRLSLRLRWGVVALAALTIAASVPLIGMVGKDFMPKDDTSDFSVALTMP
                     EGSSLAASSAMAARIEAEIKAVPGVTRIFTSIGSARGGDDVTEVELYIEIVDITERDY
                     SLTAVQKQTRDVLARYPDLRPSVQDIGGLGGGGRRTQLSFSLRGPDLEKLQEYGDDMM
                     TRMRATPGLVDVDSSAAVRKPEVRVRIDRDRAADLGVRAGEIAAALRTMIGGEPISKI
                     REGAEQYDLWLRLEPGDRRDLDTLRGLPIAARGGLVRLEALADFARERGPAQINRLGR
                     IRQIEIGANLENLPLGDAVDRVRGLTRELDLPPDYEVVFGGRAKTMEETMTSFLAALA
                     LSFLFMYMVLAAQFESFIHPVTI"
     gene            complement(2634..>3445)
                     /locus_tag="VGB85_00530"
     CDS             complement(2634..>3445)
                     /locus_tag="VGB85_00530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0132519.1"
                     /translation="QAQSDVKRAQDLGNAATGEELEQARTRLAEARAQRSLASRQLGD
                     TTVRAPFAGSISARHVAPGTYVKPGSLLFDLVAVDKLRLTLEVPERYAALVNPGTAVT
                     IGARDRVETGPPEQTDIGPRPGADRVDATVTRVSPVVSPTTRTFTVEAVFSPAGSVLR
                     PGMFIAATLALGTETAGVRVPRSAVFHVLGRDRVMIVVDGVARPQDIEMVGEDGGEAI
                     VLGLEPGVQVIARSPAQVAPGTPVTPEPISTDAAAVTPAGAPATGAGRSGP"
BASE COUNT          489 a         1209 c         1262 g          485 t
ORIGIN      
        1 aatcgtcacc gggtggatga acgattcgaa ctgcgccgcg agcaccatgt acatgaacag
       61 gaacgacagc gcgagcgcgg ccaggaacga ggtcatcgtc tcttccatgg tcttggcgcg
      121 gccgccgaac acgacctcgt agtcgggcgg caggtcgagc tcgcgggtca ggccgcgcac
      181 gcggtcgacc gcgtcaccta atggcaagtt ctcgaggttg gcgccgatct cgatctggcg
      241 gatgcggccg aggcggttga tctgcgccgg accgcgctcg cgcgcgaagt cggcgagcgc
      301 ctcgaggcgg accaggccgc cgcgcgcggc gatcggcagg ccgcgcaggg tgtcgaggtc
      361 gcggcggtcc ccgggctcga ggcgcaacca caggtcgtac tgctcggcgc cctcgcggat
      421 cttcgagatc ggctcgccgc cgatcatcgt gcgcagcgcc gccgcgatct cgccggcgcg
      481 cacgccgagg tccgccgccc ggtcgcgatc gatgcgcacg cgcacctcgg gcttgcgcac
      541 cgccgccgac gagtcgacgt cgaccaggcc gggcgtcgcc cgcatgcgcg tcatcatgtc
      601 gtcgccgtac tcttgcagct tctcgagatc gggcccgcgc agcgagaacg agagctgggt
      661 ccgccgcccg ccaccgccga ggccgccgat gtcctgcacg ctcggccgca ggtcggggta
      721 gcgggccagc acgtcgcgcg tctgcttttg caccgccgtc agcgagtagt cgcgctcggt
      781 gatgtcgacg atctcgatgt agagctcgac ctccgtgacg tcgtcgccgc cgcgggccga
      841 gccgatgctc gtaaagatgc gtgtgacccc gggcaccgcc ttgatctcgg cctcgatgcg
      901 ggccgccatc gccgagctgg ccgcgaggct cgacccctcc ggcatcgtca gcgcgaccga
      961 gaagtcgctg gtgtcgtcct tgggcatgaa gtccttgccg accatgccga tgagcgggac
     1021 gctcgcggcg atcgtcaggg ccgccagcgc gacgacgccc cagcgcaggc gcagcgacag
     1081 tcggaccatc gccgcgtagg tgttgtcgag caccgcgttg aagcgggcga ccgcgcccgg
     1141 ccggtcggat tttctcggcc tcggtcgctt gagcaggcgc gagcacagca tcggcgtcag
     1201 ggtcagcgcg acgaacagcg acaccgcgat cgcgaacgcg gtcgtgatgc cgaagctgcg
     1261 ccagaacttg ccgacgcgcc cggccatgaa cgcggtcggc aggaagatga cgatcagcga
     1321 cacggtcgtc gccacgaccg cgagcgtgat ctcgcgcgtg ccgcggatcg ccgcctcgaa
     1381 cggcgcctcg ccgtgctcga cgcggcggta caccgtctcg agcacgacga tcgcgtcgtc
     1441 gatgacgatg ccgacggcga gcgtcagcgc cagcagcgtg aagttgttga gcgtgtagtc
     1501 gagggcgtgg aggaccgcga aggtcgtgat gatcgagcac gggatcgcca gcgcggcgat
     1561 gaaggtcgag cgcagcgagc ccatgaacac cagcacggag atgctcgcca gcagcgcccc
     1621 gagcacgagg tgcagctcga cctcgtggat ggacctgcgg atgaatcttg aggcgtcgcg
     1681 gatcggcgcg accgtgatgt cggccggtag cgctcgctcg agcgcctgga cgcgcttgaa
     1741 gatcgcgtcg acgacctcga ccgtgttggt gccggcctgc ttctggatca ccagcgcgac
     1801 cgcgttctcg ccgtcgaggc gggacagcga gcgcgggtcc tcggcgctgt cctcgacctc
     1861 ggcgaccatc gccagggtga ccggcgcgcc ctgccgctcg gcgacgacga tgcgcccgag
     1921 ctcggcggcg tcctcgaccc gggccatcgt gcgcagcacg tcctcgcgtc cggggctctc
     1981 gagccggccg ccgggcagct cgaggttctg ctgctcgatc gcctggcgca cctgctggat
     2041 cgacaggccc tgcgcggtca gggcgtcggc gtcgaggtgc acgttgaacg cgcgcttgcg
     2101 cccgcccatc agcaggatct ggccgacgcc cgcgacgcct tgcaagggct ccaggacctg
     2161 cttgcgggcc agctcggtca cttctcgcag gtcgcgcgcg cgcgacgaga cggcgagcgt
     2221 cagcaccggg accgccgtgg tgtcgaagcg cgtgacgacc ggcgggtcac tgccctcggg
     2281 caggtcacgc accgcggcgg cgaccttgtc gcggacgtcc tgggtcgcct gatcgcggtc
     2341 gcggtcgagc aggaagttga ccgtcacgat cgacacaccc tcgagcgagg tcgatcgcag
     2401 ctcgtcgatg ccctcggcgg tgctgaccgc gtcctcgatg aacttggtca cctgcgtctc
     2461 gatctcctcc gggctcgcgc cgcgcagggt ggtcgtgacc gtgacgacgg ggatctcgac
     2521 gtcggggtat tgatcgaccc cgagcttgcc gaacgagagc acgccgaagg tgacgcacgc
     2581 gacgatcatc atcagcgcga agatcgggcg ccggatgcac agctcggaca gcgtcatggc
     2641 cccgacctcc ccgcgccggt cgccggggcg cccgcgggtg tgacggcggc cgcatcggtg
     2701 ctgatcggct cgggcgtcac cggcgtgccc ggggccacct gcgccgggct gcgggcgatc
     2761 acctgcacgc ccggctcgag gccgagcacg atcgcctcgc cgccgtcctc gccgaccatc
     2821 tcgatgtcct gcgggcgggc gacgccgtcg acgacgatca tcacgcggtc gcggccgagc
     2881 acgtggaaca ccgcggaccg gggcacgcgg acgccggccg tctcggtgcc gagcgcgagg
     2941 gtcgcggcga tgaacatgcc cggccgcagc acgctgcccg cgggcgagaa cacggcctcg
     3001 accgtgaagg tgcgcgtggt cggcgagacg accggcgaga cgcgggtcac cgtcgcgtcg
     3061 acccggtcgg cgcccgggcg cgggccgatg tcggtctgct cgggcggccc ggtctcgacg
     3121 cggtcacgcg cgccgatcgt gaccgcggtg ccggggttga cgagcgccgc gtagcgctcg
     3181 ggcacctcga gcgtcaggcg aagtttatcg acggcgacga ggtcgaacag cagcgagccg
     3241 ggcttgacgt aggtacccgg cgcgacgtgc cgggcggaga tcgaccccgc gaagggggcg
     3301 cgcacggtcg tgtcgccgag ctggcgcgag gcgagcgaac gctgcgcgcg ggcctcggcc
     3361 aggcgcgtgc gcgcctgctc gagctcctcg ccggtcgcgg cgttgccgag gtcctgggcc
     3421 cgcttcacgt cgctctgcgc ctgcg
//
LOCUS       DASWNJ010000025         3769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_106602, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000025 DASWNJ010000000
VERSION     DASWNJ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106602"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VGB85_00535"
     CDS             complement(<1..260)
                     /locus_tag="VGB85_00535"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEY0132520.1"
                     /translation="MQILVVTGMSGAGKSTALAALEDLGFYCADNVPVPLLPQLAQIL
                     VETDAGRRAAVGVDAREDALLRDFAGVHQHLVNAGHRVEILFL"
     gene            complement(262..1311)
                     /gene="hprK"
                     /locus_tag="VGB85_00540"
     CDS             complement(262..1311)
                     /gene="hprK"
                     /locus_tag="VGB85_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HEY0132521.1"
                     /translation="MRREASIEVSKLLEASSETLQLTLLSDWGSLDRKIAHMRIQKPG
                     LALSGFVKHVFPDRIQVLGLTEIDYLVSIPREQAEAGLAVYCQKPICAIAITRGLDPP
                     DFLMDAARRHGVPVLRTSLMSSVFISRLVRRLEELLSPRATLHGVLVDVLGVGLLLIG
                     RSGVGKSEAALDLILKGHRLVADDIVEVTVRPPDTVWGSALEMHQHHMEIRGMGILNI
                     THLFGVAAVRDNKKIEVVVELSEIQEDDYDRLGTEQQMWPILGVDVPLARIPLRPGRN
                     IASLIEVVARNQLLKYRGHDSAREFQEKINARIAASARGVNIRGTGTIPPPRPDVPVA
                     QPRVASQAMPIPEVE"
     gene            complement(1508..2170)
                     /gene="raiA"
                     /locus_tag="VGB85_00545"
     CDS             complement(1508..2170)
                     /gene="raiA"
                     /locus_tag="VGB85_00545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEY0132522.1"
                     /translation="MQTHFVFRQMESSNALRGYAEERLEKIKRYFADPLRISCTFSVD
                     KIMHCAAFDVTLRNGLQLHASEATENMYSSVDMALAKMERQVRRYKARIKHHKANEGR
                     TAKVKLGVIAGESITDHSHDDDDAHIPGGGATAANGNAGHPGQSGSDFSAPPPETEPQ
                     VIRQTEFHAERMTVQGAIMQMNLLHKQFYVFTNAASGAINVVYKAEDGNYGVIEPQPD
                     GG"
     gene            complement(2236..>3769)
                     /gene="rpoN"
                     /locus_tag="VGB85_00550"
     CDS             complement(2236..>3769)
                     /gene="rpoN"
                     /locus_tag="VGB85_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEY0132523.1"
                     /translation="ARALGAGGARLVTLVLSGSERPMALHMRQDIRMSQSLVMTPQLQ
                     QAIKLLQLSRMELSELVRDELMENPLLEEAAEIGTTAEEPVSSIELQDAVDQPNEAVA
                     EPSAGAADPITADGPPKDSVDWEVYADSTPFQPATGGGEGDEQTAFDATLTRAETLPE
                     YLLWQIRLSNYCESDEEIAEYLVRNLSPAGYLQDGSTVQVARKLGYSTLKVERVIRKL
                     QQLDPFAILSRNLAECLWVQAHHPEHPIEDPLVLAIIAKHLHNLENRNYQAVARDTGE
                     ALEEVYEATKVIQAMDPRPARAYGSEAAQYITPDVYIIKVADDFVVSLNEEGVPKLRI
                     SAMYREAMGGHPKAKDYIHERMRSAQWLIRSIQQRQRTILKVTRSILKFQREFFERGP
                     QHLRPLILKDVAEDIEMHESTVSRVTTNKYVHTPQGIFELKYFFNAGISRTSGDDLAS
                     EAVKTRIKKLIDAEDAAHPYSDQKLVELLEQEGIDIARRTVAKYREQLGVLSSSKRRK
                     LF"
BASE COUNT          555 a         1255 c         1288 g          671 t
ORIGIN      
        1 aggaacagga tctcgacgcg atgcccggcg ttgaccaggt gctgatgcac gccggcgaag
       61 tcacgcagga gcgcgtcctc gcgggcgtcg acgccgaccg ccgcgcgccg cccggcgtcg
      121 gtctccacca ggatctgcgc cagctgcggc agcaagggca cgggcacgtt gtcggcgcag
      181 tagaacccga ggtcctcgag cgccgcgagg gcggtcgact tgccggcgcc gctcatgccc
      241 gtgacgacga ggatctgcaa gctactcgac ctccgggatc ggcatcgctt gcgaggccac
      301 ccgcggctgc gcgaccggga catcgggccg cggcggtggg atcgtgccgg tgccgcggat
      361 gttgacgccg cgcgcgctgg cggcgatccg cgcgttgatc ttctcctgga actcgcgggc
      421 gctgtcgtgg ccgcgatact tcagcagctg gttgcgcgcg acgacctcaa tcagcgaggc
      481 gatgttgcga cccggccgca gggggatccg cgccagcggg acgtcgacgc cgaggatcgg
      541 ccacatctgc tgctcggtcc cgaggcgatc gtagtcgtcc tcctggatct cgctcagctc
      601 gacgacgacc tcgatcttct tgttgtcacg caccgccgcg acgccgaaca ggtgcgtgat
      661 gttgaggatc cccatgccgc ggatctccat gtggtgctgg tgcatctcca gcgcggagcc
      721 ccacacggtg tccggcggcc gcacggtgac ctcgacgatg tcgtcggcga ccaggcgatg
      781 ccccttgagg atcaggtcca gcgccgcctc gctcttgccg acgccgctgc ggccgatcaa
      841 caacagaccg acgccgagca cgtcgacgag cacgccgtgc agggtggcgc gcggcgagag
      901 cagctcctcg agccgacgca cgagccgcga gatgaagacg gagctcatca gcgaggtccg
      961 cagcaccggc acgccgtgcc gccgcgccgc gtccatcagg aagtccggcg gatcgagccc
     1021 gcgcgtgatc gcgatcgcgc agatcggctt ttgacagtac accgcgagcc cggcctcggc
     1081 ctgctcgcgg gggatcgaca cgagataatc gatctcggtc aggccgagca cctggatgcg
     1141 gtcggggaac acatgcttga caaagcccga cagcgcgagc cccggcttct ggatccgcat
     1201 gtgggcgatc ttgcggtcga gcgagcccca gtcggacagg agcgtcagct gcagggtctc
     1261 cgaggaggcc tcgagcagct tgctgacctc gatggacgct tcacggcgca tgcccgcgag
     1321 catagcaggt ggttgcggtc ctccgaccgg ggttcgcccg gcgcggtgga ggtgcgctgt
     1381 gggcgttttg gcgtgtgcgg gagcttttcg gcctcgcgcc cgtcgcggat cgccgaagct
     1441 gcaaacaagg atgccccgcg tgcttcactc gaagcgcacg gggcggccgc ccgcgaaggg
     1501 tcggctgtca gccgccgtcg ggctggggct cgatgacgcc gtagttgccg tcctcggcct
     1561 tgtagacgac gttgatcgcg ccgctggcgg cgttggtgaa cacgtagaac tgcttgtgca
     1621 gcaggttcat ctgcatgatc gcgccctgga cggtcatgcg ctcggcgtgg aactcggtct
     1681 ggcggatgac ctgcggctcg gtctccggcg ggggcgcgct gaagtcggag ccggactggc
     1741 cagggtggcc ggcgttgccg ttcgccgccg tggctccgcc gcccggaatg tgcgcgtcgt
     1801 cgtcgtcgtg cgaatgatcg gtgatgctct cgccggcgat gacgccgagc ttgaccttgg
     1861 ccgtgcgacc ctcgttggcc ttgtggtgct tgatgcgggc cttgtagcgc cggacctggc
     1921 gctccatctt cgccagcgcc atgtcgaccg aggagtacat gttctcggtc gcctcggagg
     1981 cgtgcagctg gagaccgttg cgcagcgtca cgtcgaacgc ggcgcagtgc atgatcttgt
     2041 cgacgctaaa cgtgcagctg atgcgcagcg gatccgcgaa gtagcgcttg atcttctcca
     2101 ggcgctcctc ggcatagcct ctgagcgcgt tggaggactc catctggcgg aaaacgaagt
     2161 gggtctgcat cgtcggcacc ttcttggtga tgagaacagc tgggacagct gggacagctg
     2221 ggacagccag gacagctaga acagcttgcg tcgcttcgag ctcgacagaa cgccgagctg
     2281 ctcacgatac ttggcaaccg ttcgccgggc gatgtcaatc ccctcctgct cgagcagctc
     2341 gaccagcttc tgatcgctgt acgggtgggc agcgtcctcg gcgtcgatca gctttttgat
     2401 gcgagtcttg accgcctcgc tggcgaggtc gtccccgctg gtccggctga tccccgcgtt
     2461 gaagaagtac ttcagctcga agatcccctg cggcgtgtgc acgtacttgt tggtcgtcac
     2521 ccgcgagacc gtcgactcgt gcatctcgat gtcctcggcg acatccttga ggatcaaggg
     2581 ccgcaggtgc tgcggtccgc gctcgaagaa ctcccgctgg aacttgagga tgctgcgcgt
     2641 caccttgagg atcgtgcgct ggcgctgctg gatcgaccgg atcagccact gcgccgaccg
     2701 catgcgctcg tggatgtagt ccttggcctt ggggtgcccg cccatcgcct cgcggtacat
     2761 cgcgctgatc cgcagcttgg ggacgccctc ctcgttgagg ctgaccacga agtcgtccgc
     2821 gaccttgatg atgtagacgt ccggcgtgat gtactgcgcc gcctccgagc cgtaggcccg
     2881 cgccggtcgc gggtccatcg cctggatcac cttggtcgcc tcgtagacct cctccagcgc
     2941 ctcgccggtg tcgcgcgcga cggcctgata gttgcggttc tcgaggttgt gcaggtgctt
     3001 ggcgatgatc gccagcacca gcgggtcctc gatcgggtgc tccgggtgat gcgcctgcac
     3061 ccacaggcac tccgcgaggt tgcgcgagag gatcgcaaac gggtcgagct gctgcagctt
     3121 gcggatcacc cgctcgacct tgagcgtcga atagccgagc ttgcgtgcga cctgtacggt
     3181 cgaaccatcc tgcagatagc ccgccggcga gagattacgg accaggtact cggcgatctc
     3241 ctcgtcggac tcgcagtagt tcgacagccg gatctgccac agcagatact cggggagcgt
     3301 ctcggcgcgc gtcagcgtcg cgtcgaaggc ggtctgctcg tcgccctccc cgcccccggt
     3361 cgccggctga aacggcgtgc tgtcggcgta cacctcccag tcgacgctgt ccttgggcgg
     3421 accgtcggcc gtgatcgggt cggcagcccc ggccgagggt tcggcgaccg cctcgttggg
     3481 ctggtcgacg gcgtcctgca gctcgatcga ggacaccggc tcctccgcgg tggtcccgat
     3541 ctcggcggcc tcctcgagca gcggattttc catcagctcg tcgcgcacga gctcgctgag
     3601 ctccatccgc gacagctgca gcagcttgat cgcctgctgc agctgcggcg tcatgacgag
     3661 cgactggctc atccggatgt cctgacgcat gtgcagtgcc atcggccgct cgctcccgct
     3721 gagcaccaaa gttaccagac gcgcgccgcc ggcaccaagc gcccgggcc
//
LOCUS       DASWNJ010000026         4799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_459945, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000026 DASWNJ010000000
VERSION     DASWNJ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459945"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1480
                     /locus_tag="VGB85_00555"
     CDS             161..1480
                     /locus_tag="VGB85_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132524.1"
                     /translation="MATADDPANLGVTTGQSSDDPSTGTTTDTTTGAGGCEDCSALQV
                     SAGANFTCAVVTGGRVRCWGFGDYGALGLASTERIGDDELPSSAPDVDVGGAVAEVAA
                     GGYGSGVCARLVDGAVRCWGYDQTFTGILGQMNQATIGDDESPADVPPIDLGGPATQL
                     SFGGHACALLRGGAVRCWGSNDAGRLGYGHTEDIGDDETPASAGDVDVGDTVLALAVG
                     GTHTCALLPAGRVRCWGIGQDGQLGHGNLDPLGPPELSWIGDDETPAAMGDVPVGGTV
                     VQLAAGMFHTCALLDTGGVRCWGANTFGALGHNLTHHDDIGDDETPASMGDVPIGAPA
                     IQVAAGGSHSCALLTTGAVRCWGDNSYGQLGLGHTQSLTAAQAPDIDLGGLATAIHIS
                     SDQSCAVLVDGTLRCWGRNFWGELGQGHTEDIGDDETPAQAGPIPFL"
     gene            1743..2816
                     /locus_tag="VGB85_00560"
     CDS             1743..2816
                     /locus_tag="VGB85_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132525.1"
                     /translation="MRANEARSLRATFGWGVGLASFMLLAGCSSPGGVCEDRSAPGCT
                     ACEAAPGQPQRLFGLTWNFAEGDAGSGLGTEELRCIVPGTGATDLVVGIPGMDWLPVG
                     NNAYDREGSTLYALAFANSDNITRIFSIQTVTGELLGNPAIEGELSWSGGIHVRSDGA
                     LVGVAWNLDETREEVRIIDPATGATSLIAAVPEIDSLQTATYAYDSVEDVIYMIGMAK
                     GDAATRLFAIDAEDGALLGDPAFVDAPNWSGGIHVRKDGQLVGVGWEWTDPDHTAGVE
                     KLFTIDATTAKVAPIAAIPGLLTILLGGHVYDDIADTVYIVDGDHRLFEIDATSGETL
                     AKPKLGTPKPGQEYNWSGGLHVR"
     gene            2823..3575
                     /locus_tag="VGB85_00565"
     CDS             2823..3575
                     /locus_tag="VGB85_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132526.1"
                     /translation="MAASDPQYWRTLAHSETLVRRLQGFLAGAETHESLQAWAQAQWT
                     GQDSPVCGHAVATGILMNLWNAEVRDPSFGASGPHILRRIDVVEYLWLVQRGEVNLPT
                     RDLGCLRATPAEVAARLGRETERHVLDGIGWCEYLRLASPGSGRVFMLERPLHAAARG
                     SWVRADRVADTLDVTRDLFETLTIDLADIEGFAGGFVVDPAGLPRWTLWRQDDNGIRA
                     EVADFTGRRKAEAVWRHYESLRHKQLYWLEDR"
     gene            3677..4243
                     /locus_tag="VGB85_00570"
     CDS             3677..4243
                     /locus_tag="VGB85_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132527.1"
                     /translation="MSPNRLRQLCDLLESMFQPAELQMFIYLEMDRGLLPVGAPSFEV
                     YVFQVALACEQRGIVARNELWDALVLRRPMRRPEIRRVQSLYMRPPDDDPPVPPKSGG
                     KAISWLHSLTPILQPRSRARPAPCKPAVPAETLSNAERRLCVEALATTGSIADAATLL
                     GVTRHALKRQIIKHRIEWPSGREASAAE"
     gene            complement(4304..4564)
                     /locus_tag="VGB85_00575"
     CDS             complement(4304..4564)
                     /locus_tag="VGB85_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132528.1"
                     /translation="MPIMPDLQALLRELRRVMASFTQRPHEGLAVAVGVEEVVERVVG
                     YLSADSLRRNHEGPNLYPDIVSSWRSGPYTSVGGIGWAGVCD"
     gene            4554..>4799
                     /locus_tag="VGB85_00580"
     CDS             4554..>4799
                     /locus_tag="VGB85_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132529.1"
                     /translation="MIGMIGMIGMIGIIGTTRESRTCHHGHCAAACVSSRIGANVRRR
                     ARPGRTFCHDSMERWTITEPLEGLWSMKISFGGAKPMM"
BASE COUNT          716 a         1679 c         1661 g          743 t
ORIGIN      
        1 accctgcgcg ggctcctcga tcagatcgag cccgagctcg ctggccaagc tctccatcgc
       61 cgtcctcctc gccgcctgcg gcccacagtc ggtcggctcg ctcgacgagc cggccagctc
      121 gaccacggac gcgcccgcga gcgacggcgc ggcgacgttc atggccacgg ccgatgatcc
      181 ggcgaacctc ggcgtgacga caggccagag cagcgacgat ccatcgaccg gcacgaccac
      241 cgatacgacg accggcgcgg gcgggtgtga agattgctcg gcgctgcagg tcagcgcggg
      301 cgccaacttc acctgcgccg tggtcacggg cggtcgcgtg cgctgctggg gcttcggaga
      361 ttacggcgcc ctcgggctcg ccagcaccga gcgcatcggc gacgacgagc tgccgagcag
      421 cgcgccggat gtcgacgtcg gcggcgccgt ggccgaggtc gcggccggtg gttacggctc
      481 tggcgtctgc gcgcgcctgg tcgacggcgc cgtgcgctgc tggggttacg accagacctt
      541 taccggcatc ctcggccaga tgaaccaggc gacgatcggc gacgacgagt cccccgccga
      601 tgtcccaccg atcgacctcg gcgggcccgc gacccagctg agcttcggcg gacacgcctg
      661 cgcgctgctg cgcggcggcg cggtgcgatg ctggggcagc aacgacgccg gtcggctcgg
      721 ttatggtcac accgaggata tcggcgacga cgagaccccg gcctccgcgg gtgacgtcga
      781 cgtcggcgac accgtgctgg cgctcgcggt cggcgggacg cacacgtgcg cgctgctgcc
      841 tgcgggccgc gtgcgctgct ggggcatcgg tcaggacggt cagctcggcc acgggaacct
      901 cgacccgctc ggcccgcccg agctgtcctg gatcggcgac gacgagacgc cggcggcgat
      961 gggcgacgtc ccggtcggtg gcacggtcgt ccagctcgcc gccggcatgt tccacacctg
     1021 cgccttgctc gacaccggcg gcgtgcgctg ctggggagcc aacaccttcg gcgccctcgg
     1081 gcacaacctc acgcatcacg acgacatcgg cgacgacgag acgccggcgt cgatgggcga
     1141 cgtcccgatc ggcgcccccg cgatccaggt ggcggccggg gggtcacact cgtgcgcgct
     1201 gctgacgacc ggggccgtgc gctgctgggg cgacaactcg tacggccagc tcggcctggg
     1261 ccacacgcag tcgctcacgg cggcccaggc ccccgacatc gacctcggcg gcctggcgac
     1321 cgcgatccat atctccagcg accagagctg cgccgtgctc gtcgacggga cgctgcgctg
     1381 ctggggtcgc aacttctggg gcgagctcgg ccaggggcac accgaggata tcggcgacga
     1441 cgagaccccg gcccaggccg gcccgatccc gttcctctga tccccgtcag cagccgccgc
     1501 ggcgtgctcg gccggtttga gcgcgacgta gctgcgctcg actcgcggcc acgccatcac
     1561 gcgcccatgc actccgggga tcctctccgc tataaagcat ccttcatggc tcgggcacgt
     1621 ccgctttcac gcccgaggag aagccgggct ctttcgccca cctgtacccc tgcgccggtg
     1681 gcgtcattct cgggtagcct cgcacgatgt tccgagcgac ccggcagcca ctgaattttg
     1741 cgatgcgtgc gaacgaggcc cggtcgctac gcgccacgtt cggctggggc gtcgggctgg
     1801 cgtcgttcat gctgctggcc ggttgctcga gccccggggg cgtatgtgag gatcgttcgg
     1861 cgccgggttg taccgcgtgt gaggcggcgc ccgggcagcc gcagcgcctg ttcggcctga
     1921 cgtggaattt cgccgagggc gacgctggca gcgggctggg caccgaggag ctgcgctgca
     1981 tcgtcccggg gacgggggcc accgacctgg tcgtcgggat cccggggatg gactggctgc
     2041 ccgtcggcaa caatgcgtac gaccgggaag ggtcgaccct gtatgccctc gctttcgcca
     2101 actcggacaa catcacccgg atcttctcga tccagaccgt cacgggcgag ctgctcggca
     2161 acccggcgat cgagggcgag ctctcctggt ccggcgggat ccacgtgcgg tcggacggcg
     2221 cgctcgtcgg tgtggcctgg aacctggacg agacccggga ggaggtgcgc atcatcgatc
     2281 ccgcgaccgg ggcgacgtcg ttgatcgccg cggtccccga gatcgattcg ctgcaaacgg
     2341 ccacctatgc gtacgacagc gtcgaggatg ttatctatat gatcgggatg gcgaagggcg
     2401 acgcggccac gcggctgttc gccatcgacg ccgaggacgg ggcgctgctc ggggatcccg
     2461 cgttcgtgga cgcgcccaac tggtccggag ggatccacgt ccgcaaggac gggcagctgg
     2521 tcggcgtcgg ctgggagtgg accgaccccg accacacggc gggtgtcgag aagctgttca
     2581 ccatcgacgc cacgacggca aaggtcgcgc cgatcgcggc gatccccggc ctgctgacga
     2641 tcctgctcgg cggccatgta tatgacgata tcgcggacac cgtctatatc gtcgacggcg
     2701 atcatcgcct gttcgagatc gacgccacct cgggggagac cctcgccaag cccaagctcg
     2761 gcaccccgaa gccgggtcag gaatacaact ggtccggcgg cctgcacgtc cgctgagagc
     2821 gcatggccgc cagcgaccca cagtactggc ggaccctggc ccacagcgag acgctcgtcc
     2881 gccgcctgca gggttttctc gccggggccg agacccacga atcgctgcag gcgtgggccc
     2941 aggcgcagtg gaccgggcag gacagccccg tgtgcggcca tgcggtcgcg accgggatcc
     3001 tgatgaacct gtggaacgcc gaggtgaggg acccctcctt cggagcgtcg ggtccgcaca
     3061 tcctccgccg tatcgacgtt gtcgagtatc tgtggctcgt gcagcgcggt gaggtgaatc
     3121 ttcccacccg cgacctcggc tgcctgcgcg cgactccggc ggaggtcgca gcccggctcg
     3181 gccgcgagac cgagcgccat gtcctcgacg gcatcggctg gtgcgagtac ctgcggctcg
     3241 cctcgccagg cagtggtcgc gtgttcatgc tcgagcgtcc gctccacgcc gccgcgaggg
     3301 gctcgtgggt ccgcgccgac cgggtcgcgg ataccctcga tgtcacccgc gacctgttcg
     3361 agaccctcac catcgacctt gccgatatcg agggattcgc cggtggcttc gtcgtcgacc
     3421 ccgccggcct gccgcgctgg acgctctggc gtcaggacga caacgggatc cgcgccgagg
     3481 tggcggactt caccggccgg cgcaaggccg aggccgtgtg gcgccactac gagtcgctgc
     3541 gccacaagca gctctactgg ctcgaggacc gttgaatcgt cgcggctcgt gtgtgtcaat
     3601 gtgagacaat gtgctcatgt cctggcggac cgggggtctg gattttcgcc gcctcggggt
     3661 ctaggatcgc gcctccgtgt cgccgaaccg tctccgccaa ctctgcgacc tgctggagag
     3721 catgttccag ccggcggagt tgcagatgtt catctacctg gagatggaca ggggtctgtt
     3781 accggtcggc gcgccctcgt tcgaggtgta cgtatttcag gtcgcgctgg cttgcgagca
     3841 gcgcggcatc gtcgcgcgca atgagctgtg ggacgcgctt gtgcttcgcc gaccgatgcg
     3901 tcgcccggag atccggcgcg tccagagcct ctatatgcgt cctcccgacg acgatccgcc
     3961 ggtgccacca aagtcaggcg gcaaggccat atcctggcta cactcgctga cgccgatact
     4021 ccagccacgg agtagggcgc ggcctgcccc ctgcaaaccc gcggttccgg cagagaccct
     4081 ctcgaatgca gagcggcggc tgtgcgtcga ggcgctggcg accaccggca gcatcgccga
     4141 cgcggcgacg ctcctcggcg tcacccggca cgcgctgaag cgccagatca tcaagcaccg
     4201 gatcgaatgg ccgtcggggc gcgaggcttc cgctgcagag tgagcccgct cgatcccgat
     4261 gaagcgcatc gaccagctcg cggcggtcgg ttccccgagg atttcaatcg caaacgcccg
     4321 cccacccgat cccgccgacc gaggtgtatg gcccggatcg ccagcttgac acgatgtcgg
     4381 ggtagaggtt gggcccctcg tggttgcggc gcagcgaatc ggcggagagg tacccaacga
     4441 cccgctccac gacctcctcc accccgacgg cgaccgcgag gccctcgtgc ggccgctgcg
     4501 tgaagctcgc catcacccgc cgcagctcgc ggaggagcgc ttgcaggtcg ggcatgatcg
     4561 gcatgatcgg catgatcggc atgatcggca tcatcggcac gacacgagag tctcgaacat
     4621 gtcaccacgg tcattgcgcg gcggcttgcg tgtcgtcgcg catcggcgct aacgttcgcc
     4681 gacgcgctcg cccggggcgc acattctgtc acgattccat ggaacgctgg acgatcaccg
     4741 agccgctgga aggcctgtgg agcatgaaga tcagcttcgg cggcgcgaaa ccgatgatg
//
LOCUS       DASWNJ010000027         2221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_106654, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000027 DASWNJ010000000
VERSION     DASWNJ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106654"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1406)
                     /gene="hpnE"
                     /locus_tag="VGB85_00585"
     CDS             complement(57..1406)
                     /gene="hpnE"
                     /locus_tag="VGB85_00585"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HEY0132530.1"
                     /translation="MNDPKSSVPAERVLILGAGLAGLSAAVRLAEAGRQVTVVEATKA
                     GGGRARSFPDAATGRELDNGQHLMMGCYRETLAFLRTIDSVDGIYFQRNLSVHMFKPG
                     GKKMSLDCPALPAPLHLATGLLAMRGLSLLDKAAALRAGLVLRGEVARPDDNETCDSW
                     LRRLGQTPALRNAFWDPLIWGTLNDDPLVSSAAMFTAVLERAFLSTRDASRLGVPKVP
                     LSRLYVDQAIAYLRARGSDIRVGEPVRALDVSGTQITGVTLKSGEQIEAGTVISAVPP
                     HAFLDMLSGTWPAHPVYQDIARLKTSPIVNLWFTVDKAPFTEPFVGLVGSPLHWMFNR
                     SQIEGSEGGDVLMNCTISGARACIDDPPEALQELLRGELERYFPGKVPTIRLFRAIKE
                     KRATISHAAGTYHCRPETRGPIRGLYMAGDWVRTGLPATIESAVQSGHDAAKAVLAV"
     gene            complement(1443..>2221)
                     /locus_tag="VGB85_00590"
     CDS             complement(1443..>2221)
                     /locus_tag="VGB85_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEY0132531.1"
                     /translation="QIYEFCRVVDDIVDERVPGPEGDAAARKGLEEWTAEVARIYDDP
                     YLGEDPPHTELGRGLVRTNAVFHYPREAFDEIIAGVAMDLERSTYDDMEQLRLYCYRV
                     ASCVGFLCIAIFRDQSPAARRYAEHLGLALQYTTILRDVAEDAVRGRVYLPGDLLARH
                     HLTEEDILEYRYDGRFVAAAADFAAAAEREYQAAWQILPELKHRRLLPAEIMGRTYYE
                     ILTEIRARNYNVFTRRASLRRRDKLRVAALAIARAGLSGF"
BASE COUNT          324 a          691 c          883 g          323 t
ORIGIN      
        1 tgaggtccgt cgctgaaacg atcgacgcag gtcagcacgg gccatgcggc ccggactcac
       61 acggcgagca cggccttggc ggcatcatgg ccgctctgga cggcggactc gatcgtcgcc
      121 ggaagaccgg tgcgcaccca gtcgccggcc atgtacaggc cacgaatggg accgcgggtc
      181 tcggggcggc agtgataggt gccggcggcg tgcgagatcg tcgcgcgctt ctccttgatc
      241 gcgcgaaaca ggcggatcgt cgggaccttg cccgggaagt agcgctcgag ctcgccgcgc
      301 agcagctcct gcagggcctc cggcgggtcg tcgatgcagg cgcgcgcgcc cgaaatagta
      361 cagttcatca ggacgtcgcc gccctcgctg ccttcgatct gcgagcggtt gaacatccag
      421 tgcagcgggc tgccgaccag gccgacgaag ggctcggtga acggggcctt gtcgacggtg
      481 aaccacaggt tgacgatcgg gctggtcttg aggcgggcga tgtcctgata gaccgggtgc
      541 gccggccagg tgcccgagag catgtcgagg aaggcgtgcg gcgggacggc ggagatgacg
      601 gtgccggcct cgatctgctc gcccgacttg agggtgacgc cggtgatctg ggtgccgctg
      661 acgtcgagcg cgcgcaccgg ctcgccgacg cggatgtcgc tgccgcgggc gcgcaggtag
      721 gcgatcgcct ggtcgacgta taggcggctc agcggcacct tgggcacgcc gaggcgcgag
      781 gcgtcgcgcg tgctgaggaa ggcgcgctcg agcacggcgg tgaacatcgc cgcggaggag
      841 acgagcgggt cgtcgttgag ggtgccccaa atcagcgggt cccagaaggc gttgcgcaag
      901 gcgggggtct ggccgaggcg acgtaaccag ctgtcgcagg tctcgttgtc gtcggggcgg
      961 gcgacctcgc cgcgcagcac gaggccggcg cgcagggccg cggccttgtc gagcaggctg
     1021 aggccgcgca tcgccagcag gccggtggcg aggtgcagcg gggcgggcag cgccgggcag
     1081 tcgagcgaca tctttttgcc gcccggcttg aacatgtgga cgctgaggtt gcgctggaag
     1141 tagatgccgt cgacggagtc gatcgtgcgc aggaaggcga gggtctcgcg gtagcagccc
     1201 atcatcaggt gctggccgtt gtcgagctcg cggccggtcg ccgcgtccgg gaaggagcgc
     1261 gcgcggccgc ctccggcctt ggtggcctcg acgacggtga cctgacggcc ggcctcggcg
     1321 agccggaccg ccgcggacag gcccgcgagg ccggcgccga ggatcaggac gcgctcggcg
     1381 gggacggatg acttcggatc gttcatgact gcacgactgg tggcggcggc ggacctggct
     1441 tttcagaagc cagacagtcc ggcgcgcgcg atagcgaggg cggcgacccg cagcttgtcg
     1501 cggcggcgca gcgaggcgcg gcgggtgaag acgttgtagt tgcgggcgcg gatctccgtg
     1561 aggatctcgt agtaggtgcg gcccatgatc tccgccggca gcaggcgccg gtgcttgagc
     1621 tcgggcagga tctgccatgc ggcctggtac tcgcgctcgg cggcggcggc gaagtcggcg
     1681 gcggcggcga cgaagcggcc gtcgtagcgg tactcgagga tgtcctcctc ggtcaggtga
     1741 tgacgcgcga gcaggtcccc cggcaggtac acgcggccgc ggacggcgtc ctcggcgacg
     1801 tcgcgcagga tggtggtgta ctgcagcgcg agcccgaggt gctcggcgta gcggcgggcg
     1861 gccggcgact ggtcgcggaa gatcgcgatg cacaggaagc cgacgcacga ggcgacgcgg
     1921 tagcagtaca ggcgcagctg ctccatgtcg tcataggtgc tgcgctcgag gtccatcgcc
     1981 acgccggcga tgatctcgtc gaaggcctcg cgcgggtaat ggaacaccgc gttggtgcgc
     2041 acgaggccgc ggccgagctc ggtgtggggc gggtcctcgc cgaggtaggg gtcgtcgtag
     2101 atgcgggcga cctcggcggt ccactcctcg aggcccttgc gcgcggccgc gtcgccctcg
     2161 ggtccgggca cgcgctcgtc gacgatgtcg tcgacgacgc ggcagaactc atagatctgc
     2221 t
//
LOCUS       DASWNJ010000028         1553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_565879, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000028 DASWNJ010000000
VERSION     DASWNJ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565879"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VGB85_00595"
     CDS             complement(<1..233)
                     /locus_tag="VGB85_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132532.1"
                     /translation="MNDPSFGVRLATAVATHGTLCVGIDPHPALLAQWGLTDDADGLE
                     RFAMACVAGLGGHVAVVKPQAAFFERHGSRGVAV"
     gene            complement(230..>1553)
                     /gene="carB"
                     /locus_tag="VGB85_00600"
     CDS             complement(230..>1553)
                     /gene="carB"
                     /locus_tag="VGB85_00600"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEY0132533.1"
                     /translation="PAAINLAEERGAFGRVLHEAGLNAPKHGMASSYTEALEIAARIG
                     YPVLVRPSYVLGGRGMEIVYDDEALRSYLDLAAPGLISPERPVLVDRFLDDAVEIDVD
                     ALCDGREVFIGGVMEHIEEAGVHSGDSCCTLPPVTLGRSVLEQVRRSTEAIAHGVGVR
                     GLLNVQYALHGETVYVLEANPRASRTVPFVSKATAVPLAKAAARIMLGSSIADLRAEG
                     LLPAEGDGGEQPVDAPIAVKEAVLPFNRFRTPAGQGVDPLLGPEMKSTGEVMGIDASF
                     GPAFAKSQAASYGSLPTSGRVFVSVADRDKRAMIFPVRRLADLGFEVLATGGTAEVLR
                     RHGIATTVVRKHFEGAPGDETVIDLIMDGRIDMIVNTPYGNSGPRVDGYEIRSAAVAR
                     GIPCITTVQAAAAAVQGIEAVRGDGIGVRSLQDMHAALKKSELRKARS"
BASE COUNT          217 a          609 c          529 g          198 t
ORIGIN      
        1 acggccaccc cgcgcgagcc gtgccgctcg aagaacgccg cctgcggctt gaccacggcg
       61 acgtgcccgc cgagccccgc gacgcacgcc atggcgaagc gctcgagccc gtcggcgtcg
      121 tcggtgaggc cccactgcgc cagcagggcc ggatgcgggt cgatccccac gcagagggtc
      181 ccgtgggtgg cgacggccgt ggccagccgc acgccgaacg acgggtcgtt cacgagcggg
      241 ccttccgcag ctcggacttc ttcagcgccg cgtgcatgtc ctgcagcgac cgcaccccga
      301 tcccgtcgcc gcgcaccgcc tcgatgccct gcaccgccgc cgcggcggcc tggaccgtcg
      361 tgatgcacgg gatcccgcgc gccaccgccg ccgagcggat ctcgtagccg tcgacgcgcg
      421 gcccggagtt cccgtacggc gtattgacga tcatgtcgat ccgcccgtcc atgatcaggt
      481 cgatcaccgt ctcgtcgccg ggcgcgccct cgaagtgctt gcgcaccacg gtcgtcgcga
      541 taccgtggcg gcgcaggacc tccgccgtcc cacccgtcgc cagcacctcg aagcccaggt
      601 cggccaggcg ccgcaccggg aagatcatgg cgcgcttgtc tcggtccgcg accgagacga
      661 acacccgccc ggacgtcggc agcgatccgt acgacgccgc ctgcgacttc gcgaacgccg
      721 gcccgaacga ggcgtcgatg cccatgacct cgccggtcga cttcatctcc ggccccagca
      781 gcggatcgac gccctgcccg gcgggtgtgc ggaaccggtt gaacggcagc accgcctcct
      841 tcacggcgat cggcgcgtcc acgggctgct cgccgccgtc gccctcggcg ggcaggagcc
      901 cctcggcccg caggtccgcg atcgaggaac cgagcatgat ccgggccgcc gccttggcga
      961 gcggcaccgc cgtcgccttc gacacgaacg ggacggtgcg cgacgcgcgc gggttggcct
     1021 ccagcacgta gaccgtctcg ccgtgcagcg cgtactgcac gttgagcagc ccgcgcaccc
     1081 cgaccccgtg cgcgatcgcc tcggtggacc gccgcacctg ctccagcacg gagcggccga
     1141 gggtgaccgg cgggagggtg cagcacgagt cgccggagtg caccccggcc tcctcgatgt
     1201 gctccatcac gccgccgatg aacacctcgc ggccgtcgca gagcgcgtcg acgtcgatct
     1261 cgacggcgtc gtcgaggaac cggtcgacga gcaccggccg ctcggggctg atcagcccgg
     1321 gcgcggcgag gtcgaggtag ctgcggagcg cctcgtcgtc gtagacgatc tccatcccac
     1381 gcccgccgag cacgtaggac ggccgcacca gcaccgggta gccgatccga gcggcgatct
     1441 cgagcgcctc ggtgtaggac gacgccatgc cgtgcttggg cgcgttgagg ccagcctcgt
     1501 gcagcacccg cccgaacgcc ccgcgctcct cggccaggtt gatcgccgcg ggc
//
LOCUS       DASWNJ010000029         1531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_318464, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000029 DASWNJ010000000
VERSION     DASWNJ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318464"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VGB85_00605"
     CDS             complement(<1..509)
                     /locus_tag="VGB85_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132534.1"
                     /translation="MLLVSLSWMLACAAGTDDSGGPGLSIGNITQGSQAADDTGSAGT
                     TGGDEAGSPTTTPLPDDSGYTGSGNSDPTVPTFECGNGQLDPNEECDGQELDGATCEG
                     FGFDGGTLLCSSECSYDTSMCEAAAVCGDGLLDVDTEQCDCGQGPCSAAQLGNNNCTK
                     LPSPNGAPFG"
     gene            complement(675..>1531)
                     /locus_tag="VGB85_00610"
     CDS             complement(675..>1531)
                     /locus_tag="VGB85_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132535.1"
                     /translation="GTPMGLQACLGHVLEVPWSWATVGPQKALLTAMDCKLAAQDCAG
                     VRACTPAVESFAQACKDAPLTDLCVGDTWVFCDDLGAPSLAMDCAAAGLECSKDIWAG
                     CGLEACKFGETEPKCDGDALVECSPAGWLERIDCPTQYNIVHVNGMEGEMVFSIAGET
                     CGFDEQRNALGCVGTGEACDFFSQACDGDVLETCAGGKLGRRDCAALGPAGQGCGFVQ
                     SGQFAGAAACGLVATACDLADDESCVDGKLGYCDFGQPATLDCAAAGYSGCASATLGD
                     RAVAYCTQ"
BASE COUNT          224 a          608 c          483 g          216 t
ORIGIN      
        1 ccgaacggcg cgccgttcgg gctcggcagc ttggtgcagt tgttgttgcc cagctgcgcc
       61 gcgctgcacg ggccctgacc gcagtcacac tgctcggtgt cgacgtcgag caggccatcg
      121 ccgcacaccg ccgccgcctc gcacatcgag gtgtcgtagc tgcactccga ggagcacagc
      181 aaggtcccac cgtcgaaccc gaagccctcg caggtcgccc cgtcgagctc ctggccgtca
      241 cactcctcgt tcgggtcgag ctgcccgttg ccgcactcga acgtcggcac cgtcggatca
      301 ctgttgccgc tgccggtgta gccgctgtcg tccggcagcg gcgtcgtcgt cggcgagccc
      361 gcctcgtcgc caccggtggt cccggccgac ccggtgtcgt cggccgcctg cgagccctgc
      421 gtgatgttgc cgatgctcag gcccggccca cccgagtcgt ccgtccccgc cgcgcacgcg
      481 agcatccacg acagggagac gagcagcatc cggggagagc gtcgcgcgag gcttgccact
      541 gtctcagtgt agaggccccg ccccgcgccc cacaatcccc ctcgcaatgc cccacgcgcg
      601 acccatgcgc gcgagtgctc ccccgcacga tccgcgagtt tcgccgcgca caggcggcgt
      661 caccgcgagg ctcgctattg cgtgcagtac gcgacggcgc gatcgcccag cgtcgcggag
      721 gcgcagccgc tgtagcccgc cgcggcgcag tcgagggtcg ccggctgacc gaagtcgcag
      781 tagccgagct tgccgtcgac gcagctctcg tcgtccgcga gatcacacgc ggtggcgacc
      841 agtccgcacg cggcggcccc ggcgaattgg ccgctctgca cgaagccgca accctgaccc
      901 gcgggcccca gggcggcgca gtcgcgacgc ccgagcttgc cacccgcgca tgtctcgaga
      961 acatcgccgt cgcacgcctg cgagaagaag tcgcacgcct cgcccgtgcc gacgcagccg
     1021 agcgcgttgc gctgctcgtc gaagccgcag gtctcgcccg cgatcgagaa caccatctcg
     1081 ccctccatgc cattgacgtg cacgatgttg tactgcgtcg ggcaatctat ccgctcgagc
     1141 cagcccgccg ggctgcactc gacgagcgcg tcgccgtcgc actttggctc ggtctcgccg
     1201 aacttgcacg cctcgaggcc gcagccggcc cagatgtcct tgctgcactc gagccccgcg
     1261 gccgcgcagt ccatcgccag cgacggcgcc ccgagatcgt cgcagaacac ccacgtgtca
     1321 ccgacgcaca gatcagtcaa gggcgcgtcc ttgcaagcct gcgcgaacga ctcgaccgcc
     1381 ggcgtgcacg cccgcacacc ggcgcagtcc tgcgccgcga gcttgcagtc catcgccgtc
     1441 agcaaggcct tctgcggacc gaccgtcgcc cacgaccacg ggacctcgag cacgtgcccg
     1501 aggcacgcct gcaagcccat cggcgtgcca c
//
LOCUS       DASWNJ010000030         3249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_71325, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000030 DASWNJ010000000
VERSION     DASWNJ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71325"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VGB85_00615"
     CDS             <1..882
                     /locus_tag="VGB85_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY0132536.1"
                     /translation="PFGIHTVHVLDASPQTFAMHGSKDADDLTVGELTVRASDGSNFH
                     FQDTTIIFQMNGAEAAKVIADAGVEKGYLRWMRPYARAILRDEFGKESTISVSNPAKF
                     GEATERAKSRLNELLGVHGVTVTQIVTPRPQFNEDYEKLIEERNSAGNQLEVIKSNLG
                     AAQTTRDRKLAEVNRDQNRLIQEKRTELESGLATALAEQANNTREADTYKISAIGQGQ
                     AVRSAATQQAQELRGQLDAEYRGRKSEIDAFRTQPVERVMERLGERLKGVTISIQPWA
                     NDAAPSRVEVEQIAGNP"
     gene            879..2093
                     /locus_tag="VGB85_00620"
     CDS             879..2093
                     /locus_tag="VGB85_00620"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY0132537.1"
                     /translation="MSTITRISQFICITALVLLIAPTCFITTVPLGSIGVRSSSLSGV
                     LEADLEPGWHLSVATVHNVTLLPSRYLFLDYGAEGNQGLQIRTRDNNNVFVDITVPYR
                     IRPEEAHLGMKAGTHTRTPDGKYRFQQLAENTTVGVLREDLAELTSAEFYNTDKRLEV
                     AARTLQVLNTKLAPINLEAQAVLVRAVSFRPEYEVQLQAIQLNEQNKLLDGARQKVAQ
                     QQQLLDNFELQTTALANARQQEWIKKQADLERAYQVGFVVDPNGDRAVGAARRQLAAL
                     PEEARAKLREEAARILEIEDPSKITDPYLLGIQNIQAETLEYKSRVSAEADGISGRLK
                     AEGEADLAAVRGDFETKINALLGSPAGRAYVAWKTAENVTFAQVLTFQSREGVPSVLR
                     LRNFALQFMGGS"
     gene            complement(2242..3072)
                     /locus_tag="VGB85_00625"
     CDS             complement(2242..3072)
                     /locus_tag="VGB85_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0132538.1"
                     /translation="MRPHPAPPPASTAPCRVRSAVTASPTVWCLAALLTLVTGCSTGA
                     RSNGHYRYPREAAYGRPSTCPDVRLPPLRDGGVSPDAARCSYADANGAKVTHLAGKVL
                     AEGGPGDPGTAVPEVAVTIHPVAGPVFDVDAPGPSIAHATTDAQGNYSLRGVFVAGDY
                     AIVVREPTGERITERVVRVEPNAVGSIRDLRVMVPIDPRLRAEAAGLPPASADPRLHA
                     PAAKVPAPVATSASTTPASTAPASTTPATSSPPTTPPTTPPTPRPKPSPGLRMRPPTP
                     "
BASE COUNT          536 a         1087 c         1210 g          416 t
ORIGIN      
        1 ccgttcggca tccacaccgt gcacgtgctc gacgccagcc cgcagacctt tgcgatgcac
       61 ggcagcaagg acgcggacga cctgacggtc ggcgagctga cggtccgcgc cagcgacgga
      121 tccaactttc acttccagga cacgacgatc atcttccaga tgaacggcgc cgaggcggcg
      181 aaggtgatcg ccgacgccgg ggtcgagaag ggctacctgc ggtggatgcg gccgtacgcg
      241 cgcgcgatcc tccgcgacga gttcggcaag gagtcgacga tctcggtgtc gaacccggcg
      301 aagttcggcg aggcgaccga gcgggccaag agccggctca acgagctgct cggggtccac
      361 ggggtgacgg tgacgcagat cgtcacgccg cgcccgcagt tcaacgagga ctacgagaag
      421 ctgatcgagg agcgcaacag cgccggcaac cagctcgagg tcatcaagag caacctcggc
      481 gccgcgcaga cgacccgcga ccgcaagctc gccgaggtca accgcgacca gaaccggctg
      541 atccaggaga agcgcaccga gctcgagtcg gggctggcga ccgcgctggc cgagcaggcc
      601 aacaacaccc gcgaggcgga cacctacaag atctcggcga tcggccaggg ccaggcggtg
      661 cgcagcgcgg cgacccagca ggcccaggag ctgcgcgggc agctcgacgc cgagtatcgc
      721 ggccgcaagt ccgagatcga cgcgtttcgc acgcaaccgg tggagcgcgt gatggagcgg
      781 ctcggtgagc gcctcaaggg cgtgacgatc tcgatccagc cgtgggccaa cgacgccgcg
      841 ccctcgcgcg tcgaggtcga gcagatcgcg gggaacccat gagcacgatt acgcggatca
      901 gccagttcat ctgtatcacg gcgctcgtcc tgttgatcgc cccgacctgc ttcatcacca
      961 cggtgccgct cgggtcgatc ggcgtgcgca gcagtagcct gtccggcgtg ctcgaggcgg
     1021 acctggagcc cgggtggcac ctcagcgtgg cgacggtgca caacgtgacg ctgctgccga
     1081 gccgctacct gttcctcgac tacggtgccg agggcaacca gggcctgcag atcaggaccc
     1141 gcgacaacaa caacgtgttc gtcgacatca ccgtgccgta ccggatccgg ccggaggagg
     1201 cccacctcgg catgaaggcg ggcacccata cccggacccc ggacggcaag taccggttcc
     1261 agcagctggc cgagaacacc acggtcggcg tgttgcgcga ggacctcgcc gagctgacct
     1321 ccgcggagtt ctacaacacg gacaagcggc tcgaggtcgc ggcgcgcacg ctccaggtgc
     1381 tcaacaccaa gctcgcgccg atcaacctcg aggcccaggc cgtgctggtg cgcgcggtca
     1441 gcttccgccc cgagtacgag gtccagctgc aggcgatcca gctcaacgag cagaacaagc
     1501 tgctcgacgg ggcgcgccag aaggtcgcgc agcagcagca gctgctcgac aacttcgagc
     1561 tgcagacgac cgcgctggcc aacgcgcgcc agcaggagtg gatcaagaag caggcggacc
     1621 tcgagcgggc gtaccaggtc ggcttcgtcg tcgaccccaa cggcgaccgc gcggtcgggg
     1681 cggcccgccg ccagctcgcc gcgctcccgg aggaggcgcg cgccaagctg cgcgaggagg
     1741 cggcgcggat cctcgagatc gaggatccga gcaagatcac cgacccctac ctgctcggga
     1801 tccagaacat ccaggccgag accctcgagt acaagagccg cgtcagcgcc gaggccgacg
     1861 gcatctcggg ccgcctcaag gccgagggcg aggccgacct cgcggcggtg cgcggcgact
     1921 tcgagaccaa gatcaacgcc ttgctcggca gcccggccgg ccgcgcctat gtcgcctgga
     1981 agacggcgga gaacgtcacc ttcgcccagg tcctgacgtt ccagagccgc gagggcgtgc
     2041 cctcggtgct gcgccttcgc aacttcgcgt tgcagttcat gggcggcagc tgagctggcc
     2101 tgcggggcat gtctggaagg acatgtcccg cgggacatgc ggcggacttc ggtgcctgtc
     2161 ctcggggaca tgtccggggg gtcatgcgag ccgggggacc cgccggcgcg ggatctcggt
     2221 gcctgtcctc ggggacaggg ttcagggcgt cggcggccgc atgcgcaggc cggggctcgg
     2281 cttggggcgc ggcgtcgggg gcgtcgtcgg gggcgtcgtg ggggggctgc tcgtcgcggg
     2341 ggtcgtgctc gcgggggccg tgctagcggg ggtcgtgctc gcggatgtgg cgaccggggc
     2401 cggcaccttc gcggcgggcg cgtgcagccg cggatcggcg ctcgcgggcg gcaggccggc
     2461 ggcctcggct cgcaggcgtg ggtcgatcgg gaccatcaca cgcaggtcgc ggatcgaccc
     2521 gacggcgttg ggctcgaccc ggacgacgcg ctcggtgata cgctcgccgg tcggctcgcg
     2581 gacgacgatc gcgtagtcgc cggccacgaa cacgccgcgc aggctgtagt tgccctgcgc
     2641 atcggtggtc gcgtgcgcga tcgagggccc gggcgcgtcg acgtcgaaca ccggtccggc
     2701 gacggggtgg atcgtcaccg cgacctcggg cacggcggtg ccggggtcgc cagggccgcc
     2761 ctcggcgagc accttgcccg cgaggtgggt gaccttggca ccgttggcgt cggcgtagga
     2821 gcagcgcgcc gcgtcgggcg agaccccgcc gtcgcgcaga ggggggagcc ggacatcggg
     2881 gcaggtcgag ggccggccgt aggcggcctc ccggggataa cgatagtggc cgttcgagcg
     2941 cgcgccggtg ctgcagcccg tcaccagggt gaggagcgcg gcgagacacc acaccgtcgg
     3001 actggcggtg accgcggagc ggacgcgaca gggcgccgtc gaggccggtg gtggcgcggg
     3061 gtgagggcgc atggcggcca gtgtgcccga aactcgggcg accggatcgc cggcggttga
     3121 ccgacccggg cgcatgtgtt tcgctggtct ggtatggcga acctgcccgg agatccactg
     3181 gggatcgagc tgcacagcgg tgacaacaag cagaagatcg aggaggcgct gatcaacgac
     3241 aacttcttc
//
LOCUS       DASWNJ010000031         9626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_388846, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000031 DASWNJ010000000
VERSION     DASWNJ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9626
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388846"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="VGB85_00630"
     CDS             complement(<1..787)
                     /locus_tag="VGB85_00630"
                     /inference="COORDINATES: protein motif:HMM:NF025869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA-like domain-containing protein"
                     /protein_id="HEY0132539.1"
                     /translation="MTTGPFSTVGSAPSGPPRSHFQVGGAVQQGALYVARKADRELYE
                     ALRHGEFCYILAPRQIGKSSLRIRVGQALAREQVRCVSIDFSGIGSTSVTIDEWYYSI
                     ADDIAQALGLPNLDLFWTTHQHLTPVHRWYRFIRNELLDRVSGPIVVFIDEVDSVLAL
                     SFATDDFFASIRSAYNLRADDPEYCRLTFCLLGVAAPADLIQNSVRTPFNIGREIRLD
                     DFSREELGTLRPGLEGLGCDADALLDAVYVWTSGHPYMTQRLCD"
     gene            complement(784..3000)
                     /locus_tag="VGB85_00635"
     CDS             complement(784..3000)
                     /locus_tag="VGB85_00635"
                     /inference="COORDINATES: protein motif:HMM:NF025869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA-like domain-containing protein"
                     /protein_id="HEY0132540.1"
                     /translation="MRSFGWLHFTDLHQGLDAQGWLWPGVREILFADLEKLHERCGPW
                     DLVLFSGDLTQRAAPEEFARLSQTLTQLWAHLKALGSTPSLLAVPGNHDLVRPDPRRP
                     ESLVLARWANFPEVQVEFWRDKTSPYRQLVDQAFANYAAWEQQQPFCALQRKYGMLPG
                     DFSSVVTKGDLRLGVVGLNSAFLQLTGDPYEGKLAVDVRQFHAACEGDGPAWVKNCDF
                     TLLMTHHPPDWLSPAAREHFFGEVVTPGRFAAHVYGHMHEHMTTSLSIGGSAVRRQIQ
                     GSSLFGLETFGDGKQDRRHGYSAGRITVDDRGEASLRLWPRVAKRHAAGHWRIVQDLD
                     AFELSDDQGTRPEPLPFNRKPPPPPPAPPVMSPIVTGSFNEARAAASQTAPIPDMITS
                     LSGGGVGDSGIFYTIAARVQPPGAPYNQSWHIARAEEERTALNYLDQPGAPVVLWGPE
                     KFGKTWMAQFLTNCVMQQDGGDCRVIAINLDTFDHESRADYPSFIRELAFQIVGALGG
                     PEDWVPMLWRSHGSVNQKLGRLMRENVLPLVNTRVIMALDRVDAVWGLPFKDDCFGML
                     RAWAQDPRLEKLRFILGISTTPQRLIDRPTQSPFNLTPPIVLGDFTRRQIEQIAELYG
                     LAWTDKDFHTLTDLVGGHPYLVRHVMHRANLSGGTSVSELLDEGTSLFDDFLQRYSRR
                     LHNAPELLAGVRRLGSETLSELDPEISTRLEAAGLAVEEKVGFYRLRYRLYERLRL"
     gene            3244..5940
                     /locus_tag="VGB85_00640"
     CDS             3244..5940
                     /locus_tag="VGB85_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0132541.1"
                     /translation="MRSPSRWLMIVALLAASIPGHARAQDPAELVQPVLQNSAEIVYP
                     EDLRRLDPPPQGRVVIKLVVGVDGVPRELEVSERVHPELDALALAAVQALRYSPGLYK
                     GRPIEVVLSVAIDIAAPAPLPPVDAPPEPATDPAATPGPAGATPTPESSAPVRMRGVL
                     LEAGLRTPISGASVLVVPAAADLPIGEVTKKDYGEQPAPAWTQRTFTGEDGGFELRGL
                     PEGRVRVIVLAQGFERLEYIEQIAANGRLEVKYFQRRLVVNPYRTVVASRRNEPEEVS
                     RRTISVQEINNLPGTQGDALKAIQNFPGVARPPFGAGLLVIRGAAPQDSRTYLGYHEI
                     PQLFHFGALSSTFNADILAQIDFIPGNFDSRFGDAIGGIINVNPRKGRRDGIHGYIDA
                     DVFDAGVLLEGKIKKGSFALSGRRSYIDAVLKAAVPPDAGLGLTQAPRYYDYQALFDY
                     PLGGGEFSIRWFGSDDRFKLIAKPPNDTVDTGNNSAQTAILFHRFDLVYRKQDGPWDF
                     LITPSFNFQRLSGKFGSLFSFDFPNVGFSFRAEASRQLSRRLAIRIGTELQAGRYNID
                     ARAPGFPASGGVGGTGVFNVGDIGQSYATPALYATGTIKLSERFTLLPGARLGYYAIQ
                     IQKLAVDPRLRFLWDVGEETTIKGGVGLYSQIPDLISLNPIWGNPRASVERSVHTSLG
                     VAQTFDNIGASLEVTGFYKYVFDRSTASSRIILAPDGTLRLENFANQGTGQIFGAEVL
                     LRKNLTRKFFGWVSYTLMRSLVRERPGAEYKPFAFDQPHILTVIAVYKLPRGWQIGGR
                     FRLVSGNPTTLYSDGVYDAGDDNYLPWTGKAGRLPAFHQLDLRVDKLFTYKRMLINIY
                     LDVLNVYNHQNVESIVYSYNLQQNSAQAGLPIIPSLGTRIEF"
     gene            6169..6609
                     /locus_tag="VGB85_00645"
     CDS             6169..6609
                     /locus_tag="VGB85_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY0132542.1"
                     /translation="MLTYWMDMNDPYRAFENVRRRMDQVLREYDDVAGARARASYPRA
                     SLRDTKEGFVLTAEVPGLAESDLQITATMDSLTLSGERKSPAPEGYAVHRQERGALRF
                     ARSFALPAKIDVEKITASLKHGVLTVVMPKHPESQPKSITVKAQ"
     gene            6700..7086
                     /locus_tag="VGB85_00650"
     CDS             6700..7086
                     /locus_tag="VGB85_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY0132543.1"
                     /translation="MSKDNSINTTTQSTAENTTTRPVIAPAVDIYENEHEYLVLADLP
                     GVPHDGVRIRFENGELSLDASRQDEHKDDYVGTEIVAADYRRMFRIPETVDSEKIDAK
                     LQNGVLHLVLPKASKARPRQISVKAS"
     gene            7234..8271
                     /locus_tag="VGB85_00655"
     CDS             7234..8271
                     /locus_tag="VGB85_00655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0132544.1"
                     /translation="MTTGEHGSPPPTQEELAALVPGDRIRRRPRPYIGDSVNVPLSHL
                     DPADAAVVRALYEFLGGLLGLVGPRVGGGAAGVQAVQGFLRATGFAELAQRIDRLGAA
                     LLSDDTPIDLRKVYHDVRGGGLPALLMHLDMVTEGEATPDDLERIFVLTRDQLKIIRN
                     AIPDLDPEGYAHDLEPRQHGTDLLLAKWQTEHYRVEDTEVELDLRCDFQGSVSERCME
                     FAALDRVIYNLVNNAARFADDGHVGLNVFAIDGQRDTDLRFVVMNRVAPEHRERLAAD
                     LGEELSSVFAGGYTTGGHGLGLRICGDFVVHGYGLPSLHTALDDGYLGAKLVRDTFVA
                     WFHWPARRSAA"
     gene            8411..>9626
                     /locus_tag="VGB85_00660"
     CDS             8411..>9626
                     /locus_tag="VGB85_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HEY0132545.1"
                     /translation="MNIPVLTSRIDSVTVYRQGAMVCRTAIVLAAQPVPPQVKLVNLP
                     LSLDDGSVRVRVEGEGGDLPVASDVRVALDLAVVDEDLPPPDDEALKQARRNLAEIGE
                     RIAHMQAQRQRYEQLQVFERPANKRGAPPITSPVRARLALIDFRHRAIAAIDERIQEA
                     QAQQRRAAKQKEDLEIRERAASRARQAKPHELRKAALISLRGHAIPTAPVRLVIEYMI
                     PGARWAPSYAISLSKDMTQATVSVRASVAQSSGEDWHGVKLTLSTADAQRWTELPDLQ
                     VLRIGRRQPPVQRRGWRAPPAGASELYADYDRFLSSTPGTPAAPAPAAGHARATAGSI
                     PREEESVLHSLDGSLGFAESEDMPHDGGLMARSISVQSQDSRKRDSGPPPARATRAAA
                     PPSVSRPAPPQGF"
BASE COUNT         1537 a         3446 c         3288 g         1355 t
ORIGIN      
        1 cgtcgcacag gcgctgggtc atgtacgggt ggccggaggt ccacacgtac acggcgtcga
       61 gcagcgcgtc ggcgtcgcag ccgaggccct cgaggccggg gcgcagggtg ccgagctcct
      121 cgcgcgagaa gtcgtcgagc cggatctcgc gaccgatgtt gaagggcgtg cgcaccgagt
      181 tctggatcag gtcggccggg gcggcgacgc cgagcaaaca gaaggtcagg cggcagtact
      241 cggggtcgtc cgcgcgcagg ttgtaggccg agcggatcga cgcgaagaag tcatcggtgg
      301 cgaagctcag cgcgagcacc gagtcgacct cgtcgatgaa cacgacgatc ggcccgctga
      361 cgcggtcgag cagctcgttg cggatgaagc ggtaccagcg gtgcaccggc gtcaggtgct
      421 ggtgcgtggt ccagaacagg tcgaggttcg gcaggccgag cgcctgggcg atgtcgtcgg
      481 cgatgctgta gtaccactcg tcgatcgtca ccgaggtcga gccgatgccc gagaagtcga
      541 tgctgacgca gcgcacttgc tcgcgcgcga gcgcctgccc gacgcggatc cgcaggctcg
      601 acttgccgat ctgccgcggc gcgaggatgt agcagaactc gccgtggcgc agcgcctcgt
      661 acagctcgcg gtcggccttg cgcgcgacgt acagggcgcc ctgctggacc gcgccgccga
      721 cctgaaagtg cgagcgcggc ggacccgagg gcgccgaacc cacggtcgag aacggaccgg
      781 tggtcatagc cgcagccgct cgtagaggcg gtaacgcagg cggtagaagc cgaccttctc
      841 ctcgacggcg agtccggccg cctcgagccg cgtcgagatc tccgggtcga gctccgagag
      901 cgtctcgctg ccgaggcggc gcacgccggc cagcagctcc ggcgcgttgt gcaggcgtcg
      961 cgagtagcgc tgcaggaagt cgtcgaacag gctggtgccc tcgtcgagca gttcgctgac
     1021 ggaggtgccg ccgctgaggt tggcgcggtg catcacgtgg cgcacgaggt acgggtgccc
     1081 gccgacgagg tcggtgaggg tgtggaagtc cttgtcggtc cacgcgagcc cgtagagctc
     1141 ggcgatctgc tcgatctgcc ggcgcgtgaa gtcgccgagc acgatcggcg gcgtcaggtt
     1201 gaacggcgac tgcgtcgggc ggtcgatcag ccgctgcggc gtcgtcgaga tgccgaggat
     1261 gaagcgcagc ttctccagcc gcgggtcctg ggcccacgcg cgcagcatgc cgaagcagtc
     1321 gtccttgaac ggcaggcccc acaccgcgtc gacgcggtcg agcgccatga tcacgcgcgt
     1381 gttgaccagc gggagcacgt tctcccgcat cagtcgcccg agcttttggt tgacgctgcc
     1441 gtgcgagcgc cacagcatcg gcacccagtc ctccgggcca ccgagcgcgc cgacgatctg
     1501 gaacgcgagc tcgcggatga agctgggata gtcggcccgc gactcgtggt cgaaggtgtc
     1561 gaggttgatc gcgatcacgc ggcagtcgcc gccgtcctgc tgcatcacgc agttggtcag
     1621 gaactgcgcc atccaggtct tgccgaactt ctccgggccc cagagcacga ccggcgcgcc
     1681 aggctgatcg aggtagttga gcgcggtgcg ctcctcctcg gcgcgggcga tgtgccagct
     1741 ctggttgtag ggggcgcccg gcggctggac gcgcgcggcg atcgtataaa agatgcccga
     1801 gtcgccgaca ccaccgccgc tgagcgaggt gatcatgtcg gggatcggcg cggtctgcga
     1861 ggcggccgcc cgcgcctcgt tgaacgagcc ggtgacgatc gggctcatga ccggcggagc
     1921 gggcggcggg ggcgggggct tgcggttgaa cggcagcggc tcgggccgcg tgccctgatc
     1981 gtcggacagc tcgaaggcgt cgaggtcctg gacgatgcgc cagtggcccg cggcgtgccg
     2041 cttggcgact ctgggccaca ggcgcaggct ggcctcgccg cggtcgtcga cggtgatccg
     2101 cccggccgaa tagccgtgcc ggcggtcctg cttgccgtcc ccgaaggtct cgaggccaaa
     2161 cagcgagctg ccctggatct ggcgccgcac cgccgagccg ccgatgctca gcgaggtcgt
     2221 catgtgctcg tgcatgtggc cgtacacgtg cgccgcgaag cggcccggcg tgaccacctc
     2281 gccgaagaag tgctcgcgcg ccgccggtga cagccagtcc ggcgggtggt gcgtcatcag
     2341 cagcgtgaag tcgcagttct tgacccacgc cggaccgtcg ccctcgcacg cggcgtggaa
     2401 ctgacgcacg tcgaccgcga gcttgccctc gtagggatct ccggtcagct gcaggaaggc
     2461 gctgttgagg ccgacgacgc cgagccgcag gtcgcccttg gtgacgacgc tcgagaagtc
     2521 gccgggcagc atcccgtact tgcgctgcag cgcgcaaaac ggctgctgct gctcccacgc
     2581 ggcataattg gcgaaggcct ggtcgacgag ctggcggtac ggcgaggtct tgtcgcgcca
     2641 gaactcgacc tggacctcgg ggaagttggc ccagcgcgcg agcacgaggc tctcgggtcg
     2701 gcgcgggtcg gggcgcacga ggtcatggtt gccgggcacg gcgagcaggc tcggcgtgga
     2761 gccgagggcc ttgaggtgcg cccacagctg cgtcagcgtc tggctcaggc gcgcgaactc
     2821 ctccggcgcg gcgcgctgcg tgagatcgcc ggagaacagc acgaggtccc acggaccaca
     2881 gcgctcatgc agcttttcaa ggtcggcgaa caggatctcc cgcacgccgg gccacagcca
     2941 cccctgggcg tcgaggccct ggtggaggtc cgtgaaatga agccaaccga aggagcgcat
     3001 gaagggtctc agtgtatcac ccgggtgagc caggacaggc acaagccggg ataggcgcgt
     3061 tctcgtctgc gacaacgagc ggcaggtaga cgggtcgtac gggggagatc gggccggagg
     3121 tccgggccag gggggtagta cgtccgagcc ggcgccgcgg cttccaaact tgcccgggcc
     3181 gcagcgcaat cttgcgcccg agccctaggc agaggcgcgc cccgtgagcg atgctctcgc
     3241 cacgtgcgca gcccctcccg atggctgatg atcgtggccc tgctcgcggc gagcatcccc
     3301 ggtcatgcgc gcgcccagga cccggccgag ctggttcaac ctgtcctcca gaacagcgcc
     3361 gagatcgtct accccgagga cctgcgtcgc ctcgacccac ccccccaggg ccgcgtcgtc
     3421 atcaagctgg tcgtcggcgt cgacggtgtg ccgcgcgaac tcgaggtcag cgagcgcgtc
     3481 caccccgagc tcgacgcgct cgcgctcgcg gccgtgcagg cgctgcgcta cagcccgggt
     3541 ctttataaag gccgcccgat cgaggtcgtg ctgtcggtcg cgatcgacat cgcggcgccc
     3601 gcaccgctgc cgcctgtcga cgcgcctccc gagcccgcga cagatcccgc cgcgacgccc
     3661 gggcccgcgg gcgcgactcc gaccccggag tccagcgcgc cagtgcggat gcgcggcgtg
     3721 ctgctcgagg ccggcctgcg cacgccgatc tcgggcgcca gcgtgctggt ggtcccggcc
     3781 gcggcggatc tgccgatcgg cgaggtgacc aagaaagact acggcgagca gccggcgccg
     3841 gcgtggacgc agcgcacgtt caccggcgag gacggcggct tcgagctgcg cggcctgccg
     3901 gagggccgcg tccgcgtcat cgtgctcgcg cagggcttcg agcgcctcga gtacatcgaa
     3961 cagatcgccg caaatggccg cctcgaagtg aagtacttcc agcgccggct ggtcgtgaac
     4021 ccgtaccgca cggtcgtcgc gtcgcggcgc aacgagcccg aggaggtctc gcggcggacg
     4081 atctccgtgc aggaaattaa taatctgccc ggcacccagg gcgacgcgct caaggcgatc
     4141 cagaacttcc ccggcgtcgc gcggccgccg ttcggcgccg gcctgctggt catccgcggc
     4201 gcggcgccgc aggactcgcg cacctacctc ggctaccacg agatcccgca gctgttccac
     4261 ttcggggcgc tgtcgtcgac cttcaacgcc gacatcctcg cgcagatcga cttcatcccc
     4321 ggcaacttcg acagccgctt cggcgacgcg atcggcggca tcatcaacgt caacccgcgc
     4381 aagggccggc gcgacgggat ccacgggtac atcgacgccg acgtgttcga cgccggcgtg
     4441 ctgctcgagg gcaagatcaa gaaggggagc ttcgcgctgt ccggccggcg cagctacatc
     4501 gacgcggtgc tcaaggcggc cgtgcccccg gacgccggcc tcgggctgac ccaggccccg
     4561 cgctactacg actatcaggc gctgttcgac tacccgctcg gcggcggcga gttctcgatc
     4621 cgctggttcg gatccgacga ccgcttcaag ctgatcgcca agccgccgaa cgacaccgtc
     4681 gacaccggca acaacagcgc ccagacggcg atcctgttcc accgcttcga cctcgtgtac
     4741 cgcaagcagg acggcccctg ggacttcctg atcacgccct cgttcaactt ccagcgcctc
     4801 agcggcaagt tcggctcgct gttctcgttc gacttcccca acgtcggctt ctcgttccgc
     4861 gccgaggcct cgcgccagct cagccgccgc ctggcgatcc gcatcggcac tgaacttcag
     4921 gccggtcggt acaacatcga cgcgcgcgcg cccggcttcc cggcgtccgg cggggtcggc
     4981 ggtaccggcg tgttcaacgt cggcgacatc ggccagtcct acgcgacgcc cgccctgtac
     5041 gcgaccggca cgatcaagct cagcgagcgc ttcaccctgc tgcccggcgc gcgcctcggc
     5101 tactacgcga tccagatcca aaagctggcc gtcgacccgc gcctgcgctt tttgtgggac
     5161 gtcggcgagg agacgacgat caagggcggc gtcggcctct acagccagat ccccgacctg
     5221 atcagcctca acccgatctg gggcaacccg cgcgccagcg tcgagcgcag cgtgcacacc
     5281 agcctcggcg tcgcccagac gtttgacaac atcggcgcga gcctcgaggt caccggcttt
     5341 tacaaatacg tgttcgaccg ctcgaccgcg tcgagccgca tcatcctcgc gccggacggc
     5401 acgctgcgcc tcgagaactt cgccaaccag ggcaccggac agatcttcgg cgccgaggtg
     5461 ttgctgcgca agaacttgac ccgcaagttc ttcggctggg tcagctacac gctgatgcgc
     5521 agcctcgtgc gcgagcggcc cggcgcggag tacaagccct tcgccttcga ccagccgcac
     5581 atcctcaccg tcatcgcggt gtacaagctc ccgcgcggct ggcagatcgg cggccgcttc
     5641 cggctcgtca gcggcaaccc gaccacgctg tacagcgacg gcgtgtacga cgcgggcgac
     5701 gacaactatt tgccgtggac cggcaaggcc gggcgcctgc cggcgttcca ccagctcgac
     5761 ctgcgcgtcg acaagctgtt tacctacaag cgcatgctga tcaacatcta tctcgatgtg
     5821 ctcaacgtct ataaccatca gaacgtcgag tcgatcgtgt acagctataa cctccagcag
     5881 aactcggccc aggccggcct gccgatcatc ccctcgctcg ggactcggat cgagttctaa
     5941 aaccggccgg gtgcgtcggc gcgatctccg ccggccggga cgggcggcca gcaaaacccg
     6001 tgtagcgacc ccttgacagc cccggaggcg gttttagttt ggggccaacg tcgaggcgag
     6061 cgcgcgaccc ggatgccccg ggaggtgcag caccccgaca tcaaaacacc atttttggcc
     6121 gctcgccgac gcacacaagg cgcgcgaggg ccagggagag aacgcatcat gttgacgtac
     6181 tggatggata tgaacgaccc ctaccgcgcc ttcgagaacg tccgtcgtcg gatggaccag
     6241 gtcctgcggg agtacgacga tgtcgccggc gcgagggccc gcgcgagcta cccgcgcgcc
     6301 agcctgcgcg acaccaagga gggctttgtc ctgaccgccg aggtcccggg tctcgccgag
     6361 agcgacctgc agatcacggc gacgatggac agcctgaccc tcagcggcga gcgcaagtcg
     6421 ccggcccccg agggctacgc ggtccaccgc caggagcgcg gcgcgctccg cttcgcccgc
     6481 agcttcgcgc tcccggcgaa gatcgacgtc gagaagatca ccgcgagcct caagcacggc
     6541 gtgctgaccg tcgtcatgcc gaagcacccg gagtcgcagc ccaagtccat caccgtgaag
     6601 gctcagtgag cgccggcccc cggtcgtgac ccgaaggtcc gagaccgtcc gccccagccc
     6661 tgagtcctgg tcagaacgca ccagaggagg attttcatca tgtccaaaga caactcgatc
     6721 aataccacca cccagtcgac cgccgagaac accaccaccc ggccggtgat cgcccccgcg
     6781 gtcgacatct acgagaacga gcacgagtac ctggtcctcg ccgacctccc gggcgtgccg
     6841 cacgacggcg tccggatccg cttcgagaac ggtgagctca gcctcgacgc cagccgccag
     6901 gacgagcaca aggacgacta cgtcggcacg gagatcgtcg ccgccgacta tcgccgaatg
     6961 ttccgcatcc ccgagaccgt cgacagcgag aagatcgacg ccaagctgca aaacggcgtg
     7021 ctgcacctcg tgctccccaa ggccagcaag gcccgcccgc gtcagatcag cgtcaaggcc
     7081 agctgagccg tggcgcccgg cccgccggtg cgcctgcgag cgaataaagc gagacgaacc
     7141 acacaacctg gtgagcggcg cgcttcgggc gaacatgacc cgggggcgcc gctcttgtcg
     7201 tcgtcgcgat gagctgagaa gctgacccct ccgatgacga ccggcgaaca cggctcccca
     7261 ccaccgacgc aagaagagct cgccgcgctg gtcccgggcg accgcatccg caggcggccc
     7321 cgcccgtaca tcggcgactc ggtcaacgtg ccgctgtcac acctcgaccc ggcggacgcc
     7381 gcggtcgtgc gcgcgctcta cgagttcctc ggcggcctgc tcgggctcgt cgggccgcgc
     7441 gtcggcggcg gtgcggcggg ggtgcaggcg gtgcagggct tcctgcgggc gaccggcttc
     7501 gccgagctgg cgcagcgcat cgatcgcctc ggggccgcgc tgctctcgga cgacacgccg
     7561 atcgacctgc gcaaggtcta ccacgatgtc cgcggcggcg gcctgccggc gctgctgatg
     7621 cacctcgaca tggtcaccga gggcgaggcg acgccggacg acctcgagcg gatcttcgtg
     7681 ctgacgcgcg accagctcaa gatcatccgc aacgcgatcc ccgacctcga ccccgagggc
     7741 tacgcccacg acctcgagcc gcgccagcac ggcaccgacc tgttgctggc gaagtggcag
     7801 accgagcact accgcgtcga ggacaccgag gtcgagctcg acctgcgctg cgacttccag
     7861 ggctcggtat cggagcgctg catggagttc gcggcgctcg accgcgtgat ctacaacctc
     7921 gtcaacaacg cggcccgctt cgccgacgac ggccacgtcg gcctcaacgt gttcgcgatc
     7981 gacggccagc gcgacaccga cctgcgcttc gtcgtcatga accgcgtcgc gcccgagcac
     8041 cgcgagcggc tcgcggccga cctcggcgag gagctgtcga gcgtgttcgc gggcggctac
     8101 acgaccggcg gccacggcct cggcctgcgg atctgcggcg acttcgtggt ccatggctac
     8161 gggctgccgt cgctgcacac cgcgctcgac gacggctacc tcggggccaa gctcgtgcgc
     8221 gacaccttcg tcgcgtggtt ccactggccc gcgcggcgca gcgccgcttg atcgcgcgca
     8281 tcgacgatcg agccccgacg tgggggcggt gaacatcgtt gcgcgtgcaa actgagccag
     8341 tggtgcgcgc ggagggttgg ggtaccatgc ccggcgccag gcgcctcgcg tcgcggcaac
     8401 aaggagtggc atgaacatcc cggtcctcac ctcgcgcatc gattcggtga ccgtgtatcg
     8461 ccagggggcg atggtctgcc gcacggcgat cgtgctcgcc gcccagccgg tgccgccgca
     8521 ggtcaagctc gtcaacttgc cgctcagcct cgacgacggc agcgtgcgcg tgcgcgtcga
     8581 gggcgagggc ggtgacctcc cggtcgccag cgacgtgcgt gtcgccctcg acctcgccgt
     8641 cgtcgacgag gacctgccgc cgcccgacga cgaggcgctc aagcaggccc gccgcaacct
     8701 cgccgagatc ggcgagcgca tcgcccacat gcaggcgcag cggcagcgct acgagcagct
     8761 gcaggtgttc gagcgcccgg ccaacaagcg cggcgcgccg ccgatcacct cgccggtgcg
     8821 cgcccgtctg gcgctgatcg acttccggca ccgtgcgatc gccgcgatcg acgagcgcat
     8881 ccaggaggcc caggcccagc agcggcgcgc ggccaaacaa aaagaggacc tcgagatccg
     8941 cgagcgggcc gcgtcgcggg cccgccaggc caagccacac gagctgcgca aggcggcgct
     9001 gatctccctg cgtggccacg ccatcccgac cgcgccggtt cgcctcgtca tcgagtacat
     9061 gatccccggc gcgcgctggg ccccgtcgta tgcgatctcg ctgagcaagg acatgaccca
     9121 ggcgaccgtg tccgtgcgcg cctcggtcgc gcagagcagc ggcgaggact ggcacggcgt
     9181 caagctcacc ctctcgaccg ccgacgccca gcgctggacc gagctgcccg acctgcaggt
     9241 gctccgcatc ggtcgccgac agccgcccgt gcagcgccgc ggctggcgtg cgccgcccgc
     9301 cggcgcgagc gagctgtacg ccgactacga ccgcttcctc agcagcacgc ccggcacgcc
     9361 ggccgcaccg gcaccggccg caggacatgc ccgggccacg gcggggtcga tacctcgcga
     9421 ggaggagagc gtgctccact cgctcgacgg gtcgctcggg ttcgccgagt ccgaggacat
     9481 gcctcacgac ggcgggctga tggcgcgtag catctccgtg cagtcgcagg acagtcgcaa
     9541 gcgcgactcc gggcccccgc cggcccgggc cacgcgcgcc gcggccccgc cctcggtctc
     9601 ccgcccggcg cccccgcaag gcttcg
//
LOCUS       DASWNJ010000032         7405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_601088, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000032 DASWNJ010000000
VERSION     DASWNJ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7405
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601088"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VGB85_00665"
     CDS             <1..661
                     /locus_tag="VGB85_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132546.1"
                     /translation="TKDAEDTAPAQLGTWLLAAFDVFNTAEVASLVGDGLRPVVLELD
                     DDALPEADRRLRRRLRDLKAHLRNASDLGSPGEPDEHEPADAEDDRGQATAVPSSDEV
                     PDDSLTALVRARVRGKSALFVSNRDDPDLKAKLEEKLGLDISWCDGNPRKVQAQCESI
                     ARRSYDLVLIATGFQAHMIDGILARAARAGGVPYVRVFKGRPLACVRALARTLGLHEN
                     G"
     gene            803..2017
                     /locus_tag="VGB85_00670"
     CDS             803..2017
                     /locus_tag="VGB85_00670"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEY0132547.1"
                     /translation="MKAVVFHAIGDIRLEEVADPKIEAPTDAIVRLTASAICGTDLHM
                     IRGTMPGMKKGTILGHEGVGVIEALGPDVRNFRVGDRVVIPSTLGCGYCSYCRAGYYA
                     QCDNANPNGAQAGTAFFGGPAQTGPFHGLQAEKARVPFAAVNLVKLPDEVSDEQAILL
                     SDIFPTAYFGAELAEIKPGDTVAVFGCGPVGQFCIVSARLLGAGRIFAVDHVPSRLEM
                     ARAQGAEVIDFDAEHPVETLRRLTGGIGVDRAIDAVGVDAQHARHGPAKKEAAKKAAQ
                     HSEEVEAVAPKQNPDGANWIPGDAPSQALEWAVAALCKAGTLSIVGVYPESARFFPIG
                     AAMNKNLTVKMGNCNHRKYLPHLIELVRTGAVDPAKILTQSEPLTDVLSAYKAFDTRQ
                     PGWIKVALQPIE"
     gene            2063..3340
                     /locus_tag="VGB85_00675"
     CDS             2063..3340
                     /locus_tag="VGB85_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY0132548.1"
                     /translation="MASDAVARERALEQENAALRDRCAELAAKAHYLDVVNYFAASLL
                     AAQHDLDDILWDVANNAVARLGLEDCVIYLLDDRREFLVQRAAYGPKNPRGREILAPI
                     QIPVGKGIVGAAAATGQPVLIRDTRLDPRYICDDQRRLSELALPILLDDTVIGVLDSE
                     HSQADFFTDVHREILTTIASMTASRVHRALLDDELRRAHQELEHKIVERTRELSAAIE
                     RSESLLLNTLPAPIARRLQAGEQGIAERFDEVTVLFADIVGFTPLAATMSPEALVRLL
                     GRVFTEFDNLSDALGLEKIKTIGDAYMVVAGVPTPRPDHREAMAEMALGILRAVERLN
                     HVLGTRLAVRLGMYSGSVVAGIIGARKFTYDLWGATVNIASRMESQGVSGRIHVHEST
                     YLALRGKYRFERRGILEIKGVGAMPTYFLTGPA"
     gene            4215..>7405
                     /locus_tag="VGB85_00680"
     CDS             4215..>7405
                     /locus_tag="VGB85_00680"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEY0132549.1"
                     /translation="MLLSLVAPTVHASERATAGDWRPAATGPGAIAPLAATAARGGAS
                     FYWSPTPEAARRGMSAEEAARFHVWRHRKTFSVGRAALSGVRLRFVHDQGRGGKIAVL
                     RQSVGGVELHRSDIKVLMDRDHRLLAISGAPHPGAHAASARPFTQTPVAAITGALRDL
                     HRELAAPRLIATGVTRAGYAYYQLAPTPGLRLRRPARVKPVYYPIGESLVPAHLVELQ
                     VEPAAGALEVYEYVVAADDGRVLQRNDLTAYEAFQYRTFADAGGDHRPFDGPLTDFTP
                     HPLGVPGEGPKALNPPNLVTMEGFNSNPNAVADPWLPPGATETLGNNVDAYVDHKNPN
                     GLQVDQGEFRAKVTAPGVFDYTYDVGKEPLANTTQSMAAITSLFYVTNWMHDWWYDSG
                     FVEAAGNAQADNFGRGGVGNDPLRAEAQDAALVGTRNNANMATPADGESPTMQMYLWT
                     PQVKTTLTLTPLDQSFPVNTAAFGPKNYDVTAPLVLMSDSGGVSPTDGCEAPLIDLAG
                     KIVLVDRGNCTFETKSSKAQAAGALAVIIANNADGALSPGADNMVVDPTIPTQGITKV
                     DGAALKAALAQPQTAHMFGSSSVERDGTIDNMIVAHEWGHFIHHRLVDCGANQCRAQS
                     EGWGDFNAVFMALRDGDDTAGVYGSSPYASFDTTGYYGLRRVPYSVDMTKNALSLRHI
                     GDGAALPKEHPLGPTGGPNSEVHNAGEVWATMMWEAYIAVIEAHPAIEFEALHRRMGD
                     YVVSGMMLTPSDPTFTEQRDALLLAINANDPGDFLAVAQAFAKRGAGSCAVSPPRMST
                     DFLGVVEDFELSASGTITTVTLTDDVSSCDDDGIVDAGETGTLKVTVFNHGAAAVPEG
                     STVEVTNADLPLLFPEGASVALPSIAPLGTFVASLPIAVDELFAEHLPMALELRLTTP
                     GGCEENFETFLRTDFNGDVLPASSTLDDVEAPPVWVTGGTEEGDAVWSRLASIEGHVW
                     HGADVGRVSDTWLESPTFEVSDSEPLVFTWDHLYSFEFSDNIHWDGGVVEISSDDGVT
                     WEDVTVYTKLGYDGAIASKANPLDKRLAFVDKNP"
BASE COUNT         1176 a         2875 c         2331 g         1023 t
ORIGIN      
        1 caccaaggac gccgaggaca ccgcgcccgc ccagctcggc acctggctgc tcgccgcctt
       61 cgacgtgttc aacaccgccg aggtcgccag cctcgtcggc gacggcctgc gcccggtcgt
      121 gctcgagctc gacgacgacg ccctgcccga ggccgaccgc cgcctgcgcc ggcgcctgcg
      181 cgacctcaag gcccacctgc gcaacgccag cgacctcggc agtccgggtg agcccgacga
      241 gcacgagccc gccgacgccg aggacgaccg cggccaggcg accgccgtgc cctcgtcgga
      301 cgaggtcccg gacgactcgc tcaccgcgct cgtgcgcgcg cgcgtgcgcg gcaagtcggc
      361 gctgttcgtc agcaaccgcg acgaccccga tctcaaggcc aagctcgagg agaagctcgg
      421 cctcgacatc agctggtgcg acggcaaccc gcgcaaggtc caggcccagt gcgagagcat
      481 cgcccggcgc agctacgacc tcgtgctgat cgccaccggc ttccaggccc acatgatcga
      541 cggcatcctc gcccgcgccg cgcgggccgg cggcgtgccc tacgtccgcg tgttcaaggg
      601 ccgcccgctc gcctgcgtcc gcgccctcgc ccgcaccctc ggcctgcacg agaacggctg
      661 agcagcgcgg gccgtgcgca gccatccaca tcatggcatc ggcccggcgt gaagcagccc
      721 acgggcgcag acggcccggc gtcgtccgcc gacggctggc cgcgcggcga tcgtcgcgta
      781 tgcccgccgc gagggacctg ccatgaaagc cgtcgtattt cacgccatcg gagacatccg
      841 cctcgaggag gtcgccgacc cgaagatcga ggcgccgacc gacgcgatcg tccgcctcac
      901 ggccagcgcg atctgtggca ccgacctgca catgatccgc gggaccatgc ccggcatgaa
      961 gaagggcacg atcctcggcc acgagggcgt cggcgtcatc gaggcgctcg gccccgatgt
     1021 ccgtaacttc cgcgtcggcg accgcgtcgt catcccctcc accctcggct gcggctactg
     1081 ctcgtactgc cgcgccggct actacgcgca gtgcgacaac gccaacccca acggcgccca
     1141 ggccggcacc gccttcttcg gcggcccggc ccagaccggc ccgttccacg gcctgcaggc
     1201 cgagaaggcc cgcgtcccct tcgccgccgt caacctcgtc aagctgccgg acgaggtcag
     1261 cgacgagcag gcgatcttgc tcagcgacat cttcccgacc gcctactttg gcgccgagct
     1321 ggccgagatc aagccgggag acaccgtcgc cgtgttcggc tgcggcccgg tcggccagtt
     1381 ctgcatcgtc agcgcccgcc tgctcggcgc cggccggatc ttcgccgtcg accacgtccc
     1441 ctcgcgcctc gagatggccc gcgcgcaggg ggcggaggtg atcgacttcg acgccgagca
     1501 ccccgtcgag accctccggc ggctcaccgg tggcatcggc gtcgaccggg cgatcgacgc
     1561 cgtcggcgtc gacgcgcagc acgcccgcca cggccccgcg aagaaggagg ccgccaagaa
     1621 ggccgcgcaa cacagcgagg aggtcgaggc ggtcgccccc aagcagaacc ccgacggcgc
     1681 caactggatc cccggcgacg ccccctcgca ggcgctcgag tgggccgtcg cggcgctgtg
     1741 caaggccggc acgctctcga tcgtcggcgt gtacccggag tccgcccgct tcttcccgat
     1801 cggcgcggcg atgaacaaga acctgaccgt gaagatgggc aactgcaacc accgcaagta
     1861 cctgccccac ctgatcgagc tcgtgcgcac cggcgcggtc gacccggcca agatcctgac
     1921 gcagtccgag ccgctgaccg acgtgctgtc cgcctacaag gccttcgaca cccgtcagcc
     1981 cggctggatc aaggtcgcgc tccagccgat cgagtgatcc aaagcccgac ccgcttgcgc
     2041 taccctccgc gcgaggggtc gggtggcgag cgatgcagtt gcgcgcgagc gagcgctcga
     2101 gcaggagaac gccgccctgc gggatcgctg cgccgagctg gcggccaagg cccactacct
     2161 cgacgtcgtc aactacttcg ccgcctccct gctcgcggcc cagcacgacc tcgacgacat
     2221 cctgtgggac gtcgccaaca acgccgtcgc ccgcctcggc ctcgaggact gcgtcatcta
     2281 cctgctcgac gaccgccgcg agttcctggt ccagcgcgcc gcctacggcc ccaagaaccc
     2341 gcgcggccgc gagatcctcg cgccgatcca gatcccggtc ggcaagggca tcgtcggcgc
     2401 cgccgcggcg accggccagc ccgtcctgat ccgcgatacc cgcctcgacc cgcgctacat
     2461 ctgcgacgac cagcggcgcc tctccgagct cgccctcccg atcctcctcg acgacaccgt
     2521 catcggcgtc ctcgactccg agcactcgca ggccgacttc ttcaccgacg tccaccgcga
     2581 gatcctgacg acgatcgcct cgatgaccgc gtcgcgggtc caccgcgccc tgctcgacga
     2641 cgagctgcgc cgcgcccacc aggagctcga acacaagatc gtcgagcgca cccgcgagct
     2701 gtccgccgcg atcgaacgct ccgagagcct gctcctcaac accctccccg ccccgatcgc
     2761 acgccgcctg caagccggcg agcagggcat cgccgagcgc ttcgacgagg tcaccgtcct
     2821 gttcgccgac atcgtcggct tcacccccct cgccgccacc atgtcccccg aggcgctcgt
     2881 ccgcctgctc ggccgcgtgt tcaccgagtt cgacaacctc agcgacgccc tcggcctcga
     2941 gaagatcaag acgatcggcg acgcctacat ggtcgtcgcc ggcgtcccca ccccgcgccc
     3001 cgaccaccgc gaagcgatgg ccgagatggc gctgggtatc ctgcgcgccg tcgaacgcct
     3061 caaccacgtc ctcggcaccc gcctcgccgt ccgcctgggc atgtacagcg gcagcgtcgt
     3121 cgccggcatc atcggcgccc gcaagttcac ctacgacctc tggggcgcga ccgtcaacat
     3181 cgccagccgc atggaatctc agggcgtcag cggccgcatc cacgtccacg aatccaccta
     3241 cctcgccctg cgcggcaagt atcgcttcga gcggcgcggg atcctcgaga tcaaaggggt
     3301 cggggccatg cctacttatt ttttaacggg gcctgcttga tggttcgggt ccggctctgg
     3361 cgtggcgcga acgacgtcgt acggtccggg gtgggtccgg ctgagcgccc gcgcatcctc
     3421 gagtaggcgg atgtccgacg gcgggctcgc cctcaaacac gacgcgtacg cgttggcgtg
     3481 tccgaagacc tggggcccgt cggactcggt cgagcccgcc gtcggccctc caccacccag
     3541 gccttcgcta aaagacggac ctgtcggcga agccgaagcc ggacccaccc cggccctgcg
     3601 caaacgacat ggccgtacgg gcatgtctct tcggacatgc tcgcgtcacc tggatgtcag
     3661 gacgcgcgcc gattgcaaac tgtcacccac cgatgtctca aggcgatgcc agcatgaccc
     3721 aaggaacatg tccgtgagaa catatcgtgc gcacgggcgt ggctatgatg tccgagcggc
     3781 cacaccgtat gcgcaggccg gggcgggtcc ggctctcggc tccgccgacc gcatcgacct
     3841 ttagcgaagg cctgggtggc ggagggccga cggagggctc gaccgagtcc gacgggcccc
     3901 gggccttcgg acacgcctcg aaacacgcct cgtgtttgag ggcgagccct ccgtcggaca
     3961 tccgcctact cgagcctgtg cgggcgccct gccggacctg ccccggacgt acgacggcgt
     4021 ccgcgcctcg acaaagggca tccgcgtctc cgggtctgcg cgcccccacc ggatcagccg
     4081 atgtacttaa tgtaatttac aatgcacaaa tttcaccatg tgttccagac acactgcgat
     4141 gaaacagcgt ggacatccgc gtgcataact ggcaagggtc aggtgtgctc acgatccgcc
     4201 ccctcaccgt agtgatgctc ctcagtttag tcgccccgac ggtgcacgcc agcgagcggg
     4261 ccaccgcggg agattggcgg cccgcggcga ccggcccggg ggcgatcgcg ccgctggctg
     4321 cgacggcggc gcggggcggc gcctcctttt actggtcgcc gacgccggag gccgcgcggc
     4381 gcggcatgtc ggccgaggag gcggcccggt ttcacgtgtg gcgccaccgc aagacctttt
     4441 cggtcggccg cgccgccctc tccggggttc gcctgcgctt cgtgcatgac cagggtcgcg
     4501 gcggaaagat cgccgtgctg cggcagagcg tcggcggtgt cgagctgcac cggtccgaca
     4561 tcaaggtgtt gatggaccgt gatcatcgcc tgttggcgat ctccggcgcg ccgcatccgg
     4621 gggcgcacgc cgccagcgca cggccgttca cgcagacccc ggtcgccgcg atcaccgggg
     4681 cgctgcgcga cctccatcgc gagctggcgg ccccgcgcct gatcgcgacc ggcgtcacgc
     4741 gggccggtta tgcttactat cagctcgccc cgaccccggg cctgcgcctg cgccggcccg
     4801 cgcgcgtcaa gccggtctac tacccgatcg gcgaatcgct ggtgcctgcg cacctcgtcg
     4861 agctgcaggt cgagcccgcc gcgggcgcgc tcgaggtcta cgaatacgtc gtcgccgccg
     4921 acgacggccg cgtgttgcag cgcaacgacc tcaccgcata cgaggccttc cagtatcgga
     4981 ccttcgccga cgcgggcggc gaccaccgcc ccttcgacgg tccgctgacc gactttacgc
     5041 cgcacccgct cggtgtgccc ggcgagggcc ccaaggcgct caaccctccc aacctggtca
     5101 ccatggaggg ttttaatagc aacccgaacg cggtcgccga cccctggctg ccgcctggcg
     5161 ccaccgagac cctcggcaac aacgtcgacg cctacgtcga ccacaagaac cccaacggcc
     5221 tgcaggtcga ccagggtgag ttccgtgcca aggtcaccgc gcccggcgtg ttcgactaca
     5281 cctacgacgt cggcaaggag ccgctggcga acaccaccca gtcgatggcg gcgatcacct
     5341 cgctgttcta cgtcaccaac tggatgcacg actggtggta cgactcgggc ttcgtcgagg
     5401 ccgccggcaa cgcccaggcg gacaacttcg ggcgcggcgg cgtcggcaac gacccgctgc
     5461 gcgccgaggc ccaggacgcc gcgctcgtcg gcacgcgcaa caacgccaac atggcgacgc
     5521 ccgccgacgg cgagtcgccg accatgcaga tgtacctgtg gaccccgcag gtcaagacca
     5581 ccctcacgct gacgccgctc gaccagagct tcccggtcaa cactgccgcg ttcgggccga
     5641 agaactacga cgtcaccgcg ccgctggtgc tgatgagcga cagcggcggc gtcagcccga
     5701 ccgacggctg cgaggcgccc ctgatcgacc tcgccggcaa gatcgtgctc gtcgaccgcg
     5761 gcaactgcac cttcgagacc aagtcctcga aggcgcaggc cgccggcgcg ctcgctgtga
     5821 tcatcgccaa caacgccgac ggcgcgctca gccccggcgc cgacaacatg gtcgtcgacc
     5881 cgacgatccc gacccagggg atcaccaagg tcgacggcgc cgccctcaag gccgcgctcg
     5941 cccagcccca gaccgcgcac atgttcggat cctcgagcgt cgagcgcgac ggcacgatcg
     6001 acaacatgat cgtcgcccac gaatggggcc acttcatcca tcaccgcctc gtcgactgcg
     6061 gcgccaacca gtgtcgcgcg cagagcgagg gctggggcga cttcaacgcc gtgttcatgg
     6121 ccctgcgcga cggtgacgac accgcgggcg tgtacggcag ctcgccctac gccagcttcg
     6181 acaccaccgg ctactacggc ctgcgccgcg tcccctactc cgtcgacatg acgaagaacg
     6241 cgctcagcct gcgccacatc ggcgacggcg ccgcgctgcc caaggagcac ccgctcggcc
     6301 ccacgggcgg ccccaacagc gaggtccaca acgccggcga ggtgtgggcg acgatgatgt
     6361 gggaggccta catcgcggtg atcgaggccc accccgcgat cgagttcgag gcgcttcatc
     6421 gccgcatggg cgactacgtc gtctccggca tgatgctcac cccgtccgac ccgaccttca
     6481 ccgagcagcg cgacgccctg ctgctggcga tcaacgccaa cgaccccggc gacttcctcg
     6541 cggtcgcgca ggccttcgcc aagcgcggcg ccggcagctg cgccgtctcg ccgccgcgca
     6601 tgtcgaccga cttcctcggc gtcgtcgagg acttcgagct cagcgcaagc ggcacgatca
     6661 ccaccgtcac cctcacggac gacgtgtcgt cctgcgacga cgacggcatc gtcgacgccg
     6721 gcgagaccgg cacgctcaag gtcaccgtct tcaaccacgg cgccgccgcg gtccccgagg
     6781 gctcgaccgt cgaggtcacc aacgccgacc tgccgctgct gttccccgag ggcgccagcg
     6841 tcgcgctgcc gtcgatcgcc ccgctcggga ccttcgtcgc cagcctgccg atcgccgtcg
     6901 acgagctctt cgccgagcac ctgccgatgg ccctcgagct gcgcctgacc accccgggtg
     6961 gctgcgagga gaacttcgag accttcctcc gcaccgactt caacggtgac gtcctgccgg
     7021 cgtcctcgac cctcgacgac gtcgaggccc cgccggtgtg ggtcaccggc ggcaccgagg
     7081 agggcgacgc cgtgtggtcg cgcctggcct cgatcgaggg tcacgtgtgg cacggcgccg
     7141 acgtcggccg cgtcagcgac acctggctcg agtcaccgac cttcgaagtg tccgacagcg
     7201 agccgctggt cttcacctgg gaccacctct acagcttcga gttctccgac aacatccact
     7261 gggacggcgg cgtcgtcgag atctcgagcg acgatggcgt cacctgggag gacgtgaccg
     7321 tctacaccaa gctcggctac gacggcgcga tcgccagcaa ggccaacccg ctcgacaagc
     7381 gcctcgcctt cgtcgacaaa aaccc
//
LOCUS       DASWNJ010000033         1651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_212491, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000033 DASWNJ010000000
VERSION     DASWNJ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212491"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="VGB85_00685"
     CDS             complement(<1..1128)
                     /locus_tag="VGB85_00685"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY0132550.1"
                     /translation="MDRRSFLKVAAMTGLAVAAPRTARAAFKPYAGPFYVMISARGGW
                     DPTYFCDPKPKGGPFNRLYDYDPAIDKVGNFQYANIPYPTNLVNDPLAVPYLMKAGVF
                     FAKHVGNLVVVNGLNMQTNNHDRGVVTTWSGRGDGGYPNFAALVAASRAPDHPLAFIS
                     SGGYDGTGDLIPVTRLNSVNSFRKLAEPNRINPADVNNVSTYVPASTYERIQMAQQAR
                     LAGFKAQQHLPRVAESAGQLYTARGNLDALASVDLPDTLVDIPNELGDLERMMQQTQL
                     AIAAFKAGVAVGVNLEIGGFDTHANHDTQQVRQLAKLMHGIDFVVQEATAAGIYGDMV
                     IMVGSDFGRGKGYNGVNAGDGKDHWPVSSAMFLSGNAGLIGG"
     gene            complement(1145..>1651)
                     /locus_tag="VGB85_00690"
     CDS             complement(1145..>1651)
                     /locus_tag="VGB85_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132551.1"
                     /translation="YKILFGGIDSFDITQRLTDINQIMAAIATRMAVEVSCSATAFDF
                     TKPADERLLFPLVEVEDTPDFNEGAIRKNIVHLHDRILGETLEVDDPAVDATLALFYD
                     TWAQGVKNMTDKVETNGLGPCPAVKDPTTATDLPAERQIKTDDNYTIRAWQAVIIYLL
                     SDYKFLYE"
BASE COUNT          227 a          460 c          656 g          308 t
ORIGIN      
        1 gccgccgatc aggccggcgt tgccgctgag gaacatcgcg ctgctgaccg gccagtggtc
       61 cttgccgtcg cccgcgttga cgccgttgta gcccttgccg cgcccgaagt cggagccgac
      121 catgatcacc atgtcgccgt agatgccggc ggcggtcgcc tcctggacca cgaagtcgat
      181 gccgtgcatc agcttggcga gctggcggac ctgctgggtg tcgtggttgg cgtgggtgtc
      241 gaagccgccg atctcgaggt tgacgccgac ggcgaccccg gccttgaacg ccgcaatcgc
      301 cagctgggtc tgctgcatca tgcgctcgag gtcgccgagc tcgttgggga tgtcgacgag
      361 cgtgtcgggc aggtcgaccg aggcgagcgc gtcgaggttg ccgcgggcgg tgtagagctg
      421 gccggcggac tcggcgacgc gcggcaggtg ctgctgggcc ttgaacccgg cgaggcgggc
      481 ctgctgggcc atctggatgc gctcgtaggt cgaggcgggc acgtaggtgc tgacgttgtt
      541 gacgtcggcc gggttgatgc gattgggctc ggcgagcttg cggaacgagt tgaccgagtt
      601 gagccgcgtg acggggatca ggtcgccggt gccgtcgtag ccgccggagc tgatgaaggc
      661 gagcgggtgg tcgggggccc tgctggcagc gacgagcgcc gcgaagttgg ggtacccgcc
      721 gtcgccgcgg cccgaccacg tggtcacgac gccgcggtcg tgattgttgg tctgcatgtt
      781 gaggccgttg accacgacga ggttgccgac gtgcttggcg aagaagacgc cggccttcat
      841 caggtacggg accgcgagcg ggtcgttgac gaggttggtg gggtacggga tgttggcgta
      901 ctgaaagttg ccgactttat cgatcgcggg gtcgtagtcg tagaggcggt tgaaggggcc
      961 gcccttgggc ttggggtcgc agaagtaggt cgggtcccag ccgccgcggg ccgagatcat
     1021 gacgtagaac ggccctgcgt agggtttgaa ggcggcgcgg gcggtgcggg gggctgcgac
     1081 ggcgagcccg gtcatcgcgg cgactttgag gaagcttcgg cggtccatgg tcgtctcctg
     1141 gggctcactc gtagaggaac ttgtagtccg agagcagata gatgatgacg gcctgccacg
     1201 cgcggatcgt gtagttgtcg tcggtcttga tctggcgctc ggcgggcagg tcggtggcgg
     1261 tggtcgggtc cttgacggcc gggcaggggc cgaggccgtt ggtctcgacc ttgtcggtca
     1321 tgttcttcac gccctgcgcc caggtgtcgt agaacagcgc gagggtggcg tcgacggcgg
     1381 ggtcgtcgac ctcgagggtc tcgccgagga tgcggtcgtg caggtgcacg atgttcttgc
     1441 ggatcgcgcc ctcgttgaag tcgggcgtgt cctcgacctc gacgagcggg aacagcaggc
     1501 gctcgtcggc cggcttggtg aagtcgaagg cggtcgcgct gcacgagacc tcgacggcca
     1561 tgcgcgtggc gatcgcggcc atgatctggt tgatgtcggt caggcgctgg gtgatgtcga
     1621 aggagtcgat gccgccgaac aggatcttgt a
//
LOCUS       DASWNJ010000034         1772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_177277, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000034 DASWNJ010000000
VERSION     DASWNJ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177277"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..>1772
                     /locus_tag="VGB85_00695"
     CDS             28..>1772
                     /locus_tag="VGB85_00695"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0132552.1"
                     /translation="MRERDWSTSLLGAPETWPQALRPVVGLMLGSRYPMFAAWGPELA
                     FLYNDAYAPILGSKHPHALGGRFQEVWSEIWGDLEPLVRKALAGEATFHENLHLVMQR
                     NGYPEDTWYNFSYSPIQDETGAVAGMFCACTETTAQVLAERRLANESERQRRLFQQAP
                     GFITILNGPEHVFEFVNEAYRRAFGDRNFVGKTVREAFPELENQTFFGLLDEVYADGE
                     RFVANGVPIRLQALPEAEPEERFLDFIYEPVKNDKGQVTGIFCEGYDVTEAIRAQASL
                     RASEEFNRRILASSSDCIKVLALDGKLETMSDGGRQSLAISDLGPFIGTEWPEWFEPE
                     GRAAARAGVAEAREGRTARFEAKLRTQNGVRRDWETVLTPVFGERGRPERILALSRDI
                     TERLEAEGLLRESEARRAFLLTLGDLTRELSDPTSIVEATTRALGEHLRANRVVYAEI
                     DEAAGLATTCGGWTEGDAPRLPAVVHLADYGERLIARLRAGEALCACDVRADQDTRGS
                     LVALEAIGVLALISVPVFRDGRLVANLNVHQDLPRLWTDAETEMVGAVAERTFEAIER
                     AKADAALRTSEARFAA"
BASE COUNT          323 a          535 c          597 g          317 t
ORIGIN      
        1 cctgacgatc gcgaactggg ccgactcatg cgggaacgcg attggtcgac gtcactgctc
       61 ggtgcccccg aaacatggcc gcaagcgctg cgtccggttg tcgggctgat gctcggctca
      121 cgctacccca tgttcgctgc ctggggtcct gaactcgcct tcctgtacaa cgacgcctac
      181 gcgcccatcc tcggctcgaa gcacccgcac gctctcggcg ggcggttcca ggaggtctgg
      241 tcggagatct ggggcgacct cgagccgctc gtgaggaagg cgctggctgg agaggcgacc
      301 ttccacgaga acctgcatct tgtgatgcaa cgcaacggct atccggagga tacctggtac
      361 aacttctcct actcaccgat acaggatgag acgggtgcag tcgccggcat gttctgcgcc
      421 tgcactgaga cgacggcgca agtgctcgcc gaacggcgtc ttgccaacga gagtgagcga
      481 cagcggcgac ttttccagca ggcgccgggg tttatcacga ttctgaacgg accggagcac
      541 gtcttcgagt ttgtgaatga agcgtaccgc cgggcctttg gcgaccgaaa ctttgttggc
      601 aagacggtcc gagaggcttt ccctgaactc gagaaccaaa ctttcttcgg cttgctcgac
      661 gaggtttacg ctgacggcga gcggttcgtg gcgaacggtg ttccgatccg cctccaagct
      721 ctgccggaag cggagccgga agagcgattc ctcgatttta tctacgagcc ggtgaagaac
      781 gacaaggggc aggtcaccgg aatcttctgc gagggctacg acgtcaccga agcgatccgt
      841 gcccaagcat cgctgcgggc cagtgaggaa ttcaatcgtc ggatcctcgc aagctccagc
      901 gactgcatca aagtcttggc cttggacggc aagctggaga cgatgagcga cggtggccga
      961 cagtcgcttg caatcagcga tctgggaccg ttcatcggaa cagaatggcc cgagtggttc
     1021 gaacccgagg ggcgcgcggc ggcacgagcg ggtgtcgctg aggcgcgcga aggccggacg
     1081 gcgcgtttcg aggccaaact gcggacgcag aacggcgtcc gacgggattg ggagacagtg
     1141 ctgacacctg tcttcgggga gcgtggacgc cctgagcgga tcctagcgct ctcgcgggac
     1201 atcacggaac gtcttgaagc cgaagggctc ttgcgggaga gcgaggctcg gcgtgccttt
     1261 cttctcactc tgggcgacct gacgcgcgag ctttctgatc ccacgagcat cgtggaggcg
     1321 acgacccgtg cactcggtga gcatctgcgc gccaaccgtg tggtctatgc cgaaatcgac
     1381 gaggcggcgg gtctcgcaac aacttgcggt ggctggacag agggcgacgc accccgactg
     1441 ccggccgttg tccacctcgc cgattacggt gagcgcctca ttgcgcggct gcgggccggg
     1501 gaggcgctgt gcgcctgcga cgtccgagct gaccaagata ctcggggcag tctggtggcg
     1561 ctcgaagcga tcggcgtcct ggcgctgatc agcgtgcccg tcttccggga cgggcgcttg
     1621 gtcgcgaacc tcaacgttca tcaagatctt ccgcgcctct ggacggatgc cgagactgag
     1681 atggttgggg cggtcgccga acgcacgttt gaggcgatcg agcgtgccaa ggcagacgca
     1741 gcgctccgaa cgagcgaggc acgcttcgcg gc
//
LOCUS       DASWNJ010000035         2251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_388877, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000035 DASWNJ010000000
VERSION     DASWNJ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388877"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..407
                     /locus_tag="VGB85_00700"
     CDS             180..407
                     /locus_tag="VGB85_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132553.1"
                     /translation="MSRRAGGGARPYVVRRYVARQSVYAKAFADLLPLTFDAAIARAV
                     NEPITPTDEARTEATHQASRREREALDRSRD"
     gene            complement(411..1208)
                     /locus_tag="VGB85_00705"
     CDS             complement(411..1208)
                     /locus_tag="VGB85_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY0132554.1"
                     /translation="MLGLTGVGVLGGCDEAEEPNSAIADDPGSIDGIDGIDGISMEDE
                     RGLADADEIESRAGGSCQGFCGSQSSAGCWCDSGCAQYGDCCADKAQVCDSAPPPPPP
                     PPPAPSSDIPNNTYCADVISWPQTSVDFEAQVLVLVNQRRAAGATCPTYGYKAPAPAL
                     TMNGALRCAARKHDKDMLANNFFSHTGTGGTTPWQRIASAGYGAYKAAAENIAAGQAT
                     PAAVVTGWMNSAGHCKNIMDPALKEIGVGHATGGAYGHYWTQDFASK"
     gene            complement(1555..>2251)
                     /locus_tag="VGB85_00710"
     CDS             complement(1555..>2251)
                     /locus_tag="VGB85_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132555.1"
                     /translation="KREQLKARAAEAKGEQEQARALAELRAQAELVSERAAREQARQL
                     AELRARTELEAEKAQQAEAARLAQLAREKKLAEAERAVLETRHQNAAREAELAGLLAA
                     QKARAAAELREQESVAAAKVAQRELEVKRLAGEVTAAAARAAKEIENMISDDRIRLAL
                     VDTGLPAVASAFAQKFGEVKFTSIGSGDGDPSAMIGRGIAQVMNLVKDVGAEVLGART
                     PVTIPAPPQRPAT"
BASE COUNT          276 a          829 c          791 g          355 t
ORIGIN      
        1 ggcgcgtcga ggtcggcggg gtcgggccgc acggggtcgc ggtggtgctc gacggccgcg
       61 tgcagatgca cgtcccacgc gaggtcgccg acgcgatcgc acaggcccgc gctcgtcatc
      121 cgcgctgacg caggccccgc acccggggac gcctcgcggg cgggagctgg cggcgcgcgg
      181 tgtcgcggcg cgcaggcggc ggggcgcgcc cgtacgtggt ccggcgctat gttgctcgcc
      241 agagcgtcta cgccaaggcg ttcgccgacc tgctgccgct gaccttcgac gccgcgatcg
      301 cccgcgcggt caacgagccg atcacgccga ccgacgaggc gcgcaccgag gcgacgcacc
      361 aggcctcgcg tcgtgaacgc gaggccctcg atcgctcgcg cgattgaccg ttacttgctg
      421 gcgaagtcct gcgtccagta gtgcccgtag gcgccaccgg tcgcatgccc gacgccgatc
      481 tccttgagcg ccggatccat gatgttcttg cagtgaccgg cgctgttcat ccagccggtg
      541 acgaccgcgg ccggcgtggc ctgaccggcg gcgatgttct ccgccgcggc cttgtacgcg
      601 ccataacccg cgctggcgat ccgctgccac ggcgtcgtgc cgccggtgcc ggtgtgcgag
      661 aagaagttgt tggcgagcat gtccttgtcg tgcttgcgcg cggcgcagcg cagcgcgccg
      721 ttcatcgtca gcgcgggcgc gggcgccttg tagccgtagg tcgggcaggt cgcaccggcg
      781 gcgcggcgct ggttgacgag cacgagcacc tgcgcctcga agtcgaccga ggtctgcggc
      841 cagctgatca cgtcggcgca gtaggtgttg ttcgggatgt cgctgctcgg cgccggcggg
      901 ggcggcgggg gcggaggcgg cgcggagtcg cagacctgcg ccttgtcggc gcagcagtcg
      961 ccatactgcg cgcagccgct gtcgcaccag caccccgccg aggactgcga gccgcagaag
     1021 ccctgacagc tgccacccgc gcgcgactcg atctcgtccg cgtccgcgag gccgcgctcg
     1081 tcttccatgc tgatgccgtc gatgccatcg atgccgtcga tgctgccagg gtcgtccgcg
     1141 atcgcgctgt tgggctcctc tgcctcgtca cagccgccca gcacgccgac gccggtcaag
     1201 ccgagcacgg cacaaaacat ccaccgtgcg ttcttgtgat ctgcaaacat ctccaaccct
     1261 ccactttccc tcctacaccc ctctgggatg cgttacaagc cataattcac cggggcctcg
     1321 aggctacgaa gcggcgccga gacgtggatg cgccgcccca ggtcatgccc ccatcgccaa
     1381 cagcgccggg gtctgctcgc cgcagtcgtg acgggcgaca cgcctgtcgc atctgcaacg
     1441 acatcgcccg gtcatcgcgt ctgcgataac catgatgcac tgctgcgctg cgatccacgg
     1501 cggcacaaaa agggacatgt cctcacggac atgtcccttc gttcacaggc gccatcaggt
     1561 cgccggacgc tgcggcggcg cggggatcgt gaccggcgtg cgggcgccga gcacctcggc
     1621 gccgacgtcc ttgaccaggt tcatcacctg cgcgatgccg cggccgatca tcgccgaggg
     1681 gtcgccgtcg ccgctgccga tcgaggtgaa cttcacctcg ccgaacttct gcgcgaaggc
     1741 cgaggcgacc gcgggcaggc cggtgtcgac cagcgcgagg cggatgcggt cgtcgctgat
     1801 catgttctcg atctccttcg ccgcgcgcgc tgcggcggcc gtgacctcgc cggcgaggcg
     1861 cttcacctcg agctcgcgct gggccacctt ggccgcggcg acgctctcct gctcgcgcag
     1921 ctcggcggcg gcgcgggcct tctgggccgc gagcagtccg gccagctcgg cctcgcgggc
     1981 ggcgttctgg tgccgcgtct cgagcaccgc gcgctcggcc tcggcgagct tcttctcgcg
     2041 ggcgagctgg gccaggcggg cggcctcggc ctgctgcgcc ttctcggcct cgagctcggt
     2101 gcgcgcgcgc agctcggcga gctggcgggc ctgctcgcgc gccgccctct cgctgaccag
     2161 ctcggcctgc gcgcgcagct cggcgagcgc acgcgcctgc tcctgctcgc ccttggcctc
     2221 ggcggcgcgg gccttgagct gctcgcgctt g
//
LOCUS       DASWNJ010000036         3119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_671813, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000036 DASWNJ010000000
VERSION     DASWNJ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671813"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VGB85_00715"
     CDS             complement(<1..782)
                     /locus_tag="VGB85_00715"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0132556.1"
                     /translation="MFVAPAADPPSQPVIAGRFVLERLLGAGNMGDVFLALDRETGQR
                     VALKVLKPADAPPEIDRFIREAQLLTELRHPNIVAHVAHGVDVARGPYLAMELLEGED
                     LRQHLCRGRLSVGDTVALLHGVAAALAEVHRFGVVHRDLKPSNLFLRHGEPRNVVLLD
                     FGIARRALRGGALTRTGAIVGTPEYMAPEQARGARGIGPPTDVFALGCVIHECLTGQP
                     VFHGEHIAAVLVKILFEGPAPLERADVPDALAELLHRMLAKDP"
     gene            complement(870..>3119)
                     /locus_tag="VGB85_00720"
     CDS             complement(870..>3119)
                     /locus_tag="VGB85_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132557.1"
                     /translation="GLAAALLVRRWARGRDLAPRVLTIDPNLAVTRPDGPEALAKAVR
                     EDMARETPALDPTGVDVSRDPDGELLREPWLLHLVTFGAGFAGVGLEVVGVQVLAQNL
                     ENTVYTFANILAVYLIGTALGSAIYQRLAARAVAGRPGGVTLGLLAAMALLVIPAAFA
                     LQASPALLAALLPEHPGLARSVLAELTVAAVVFLPATIVMGALFAHVTGLISAAGRGV
                     GRAYALNTLGGALAPFVFGLWAMPRLGYADTLYLVLYAYLVVFGLFGWYRRFHPALLV
                     GCALLAIALTTAAPRSLGLLHAEPGWTVLDRRETLHGLVLVSERPATPAAAGQPATPA
                     LRRLQVGKHFKMGGAFSFGEHRMGHLCAMLGAASSPIPVQKAMFLGIGTGATMGAALQ
                     MVDAEADGVELVPEVLGLLPRFADINHDLASRARTNLHAADARRFVVASPGQYDLALA
                     DLFHPGQDGAGSLYAEEHFIAVREHLRPGALFCQWLPLYQLGPEELRSVTRTFLGVFP
                     EVHAFLGLYNIGTPSLALVGRVPTDRAPALRLDRARLDHLADHPSATLDLRDLLASYL
                     ADGAALRAWAGDGPSNSDLRPSVMFDAPVSAYADDPTLGARNLEELLALRPADPTVLL
                     ADPAATDLRSQVRTYTAAADLYLRAELARGEHTDLGTLPPEVAELLLKAYEADPAFAP
                     ARGPLYAIAGKNPTLAEMLLPRMLARTPDEPRVHQAWLRHLQAIGDRPRFDQALQDAQ
                     GRFPAAPQP"
BASE COUNT          451 a         1004 c         1287 g          377 t
ORIGIN      
        1 gggtccttgg cgagcattcg gtgcagcagc tccgcgagcg cgtcgggtac gtcggcgcgc
       61 tcgagcggcg ccggcccctc gaagaggatc ttcacgagca ccgcggcgat gtgttcgccg
      121 tggaacaccg gctgcccggt caggcactcg tggatcacgc agccgagggc gaagacgtcg
      181 gtcggcggtc cgatgccgcg ggcgcctcgc gcctgctccg gcgccatata ctccggcgtg
      241 ccgacgatcg ccccggtgcg ggtcaaggcg ccgccgcgca gggcccgtcg cgcgatcccg
      301 aagtcgagca ggacgacgtt gcgcggctcg ccgtggcgca ggaacaggtt gctcggcttg
      361 aggtcgcggt ggacaacgcc gaagcggtgg acctcggcga gcgccgcggc gacgccgtgc
      421 agcagcgcga ccgtatcgcc gacggagagc cgcccccggc acaggtgctg gcgcaggtcc
      481 tcgccctcga gcagctccat cgccaggtac ggcccgcggg ccacgtcgac cccgtgggcg
      541 acgtgcgcga cgatattcgg gtggcgcagc tcggtcagca gctgcgcctc gcggatgaag
      601 cggtcgatct ccggcggggc gtcggcgggc ttgaggacct tcaacgcgac gcgctgcccg
      661 gtctcccggt cgagcgcgag gaagacgtct cccatgttgc cggccccgag cagacgctcg
      721 agcacgaagc ggccggcgat caccggttgg gacggcggat ccgctgccgg tgcgacgaac
      781 atggccggca gtatagggat ttgccgcccc ggccgcgccc gtggcgacgg cgcggaaccg
      841 ctctggagga acgggccggt ccgccccggt cagggctgcg gggccgcggg gaagcggccc
      901 tgcgcgtcct gcagcgcctg gtcgaagcgc gggcggtcgc cgatcgcctg gaggtggcgc
      961 agccaggcct ggtgcacgcg cggctcgtcg ggcgtgcgag ccagcatgcg cgggagcagc
     1021 atctccgcga gcgtcgggtt cttgccggcg atcgcataca gcgggccgcg tgcaggcgcg
     1081 aacgcagggt cggcctcgta tgcctttaat aagagctcgg cgacctcggg ggggagggtg
     1141 ccgaggtcgg tgtgttcacc gcgggccagc tcggcgcgca ggtagaggtc ggcggcggcg
     1201 gtatatgtgc ggacttgaga tcgcaggtcg gtcgcggcgg ggtcggcgag caggacggtg
     1261 gggtcggcgg ggcgcagcgc gagcagctcc tcgaggttgc gcgcgccgag ggtcgggtcg
     1321 tcggcgtagg cgctgacggg ggcgtcgaac atgacgctcg ggcgcaggtc ggagttgctc
     1381 ggaccgtcgc cggcccacgc gcgcagggcg gcgccatcgg ccaggtagga ggcgagcagg
     1441 tcgcgcaggt cgagggtcgc ggagggatgg tccgcgaggt ggtcgaggcg cgcgcggtcg
     1501 aggcgcagcg cgggggcgcg gtcggtcggc acgcggccga cgagcgcgag cgacggggtg
     1561 ccgatattgt agagccccag gaatgcgtgc acttcgggga atacaccgag gaaggtgcgg
     1621 gtgacggagc gcagctcctc ggggccgagc tggtacaggg gcagccactg gcagaacagc
     1681 gcgcccgggc gcaggtgctc gcgcacggcg atgaagtgct cctcggcgta cagcgagccg
     1741 gcgccgtcct ggccggggtg gaacaggtcg gcgagggcga ggtcgtactg tccggggctg
     1801 gcgacgacga agcggcgcgc gtcggcggcg tgcaggttgg tgcgggcgcg gctcgcgagg
     1861 tcgtggttga tgtcggcgaa gcgcgggagc agccccagca cctcgggcac gagctcgacg
     1921 ccgtcggcct cggcgtcgac catttgcagg gcggcgccca tggtcgcgcc ggtgccgatc
     1981 ccgaggaaca tcgccttttg cacggggatc gggctggagg cggcgccgag catcgcgcac
     2041 aggtgaccca tgcggtgctc gccgaagctg aaggcgccgc ccatcttgaa gtgcttgccg
     2101 acctgtaatc ttcgcaatgc gggcgtcgcc ggctggccgg cggccgcggg ggtcgcaggg
     2161 cgctcgctga cgagcacgag gccgtgcagg gtctcgcggc ggtcgagcac ggtccagccg
     2221 ggctcggcgt gcagcaggcc gagggaccgc ggggccgcgg tcgtcaacgc gatcgcgagc
     2281 agcgcacagc cgacgagcag ggccgggtgg aagcggcgat accagccgaa caggccgaac
     2341 acgacgaggt acgcatatag tacgaggtac agcgtatcgg cgtagccgag ccgcggcatc
     2401 gcccataatc cgaacacgaa gggggccagg gcgccgccga gggtattgag ggcataggcg
     2461 cggccgaccc cgcgtccggc ggcggagatc aggccggtga cgtgggcgaa cagggcgccc
     2521 atcacgatcg tcgccggcag gaacacgacg gcggccacgg tcagctcggc gagcaccgag
     2581 cgggcgaggc cggggtgctc cggcagcagc gcggcgagca aggcgggcga ggcctgcagc
     2641 gcgaacgcgg cggggatcac cagcagcgcc atcgcggcga gcaggccgag ggtcacgccg
     2701 ccgggccggc cggcgaccgc ccgcgcggcg aggcgctggt agatcgcgga gccgagtgcg
     2761 gtgccgatca aatagacggc gaggatgttg gcaaaggtat agacggtgtt ctcgaggttc
     2821 tgcgcgagca cctgcacgcc gacgacctcg aggccgacgc ccgcgaagcc ggcgccgaag
     2881 gtgacgaggt ggagcagcca cggctcgcgc agcagctcgc cgtcggggtc gcgcgagacg
     2941 tcgacgccgg tcgggtcgag cgcgggtgtc tctcgggcca tgtcctcgcg gacagccttc
     3001 gccagcgcct cggggccgtc ggggcgggtg accgcgaggt tcgggtcgat cgtgaggacc
     3061 ctcggggcga ggtcgcggcc gcgggcccag cggcgcacga gcagggccgc ggcgaggcc
//
LOCUS       DASWNJ010000037         2541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_71505, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000037 DASWNJ010000000
VERSION     DASWNJ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71505"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VGB85_00725"
     CDS             <1..260
                     /locus_tag="VGB85_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY0132558.1"
                     /translation="VWAIGTGKTATPRDAASMHRRLRAWLEQQFGAAGADRSILYGGS
                     VKPNNAAALMAAGDVDGFLVGGASLEAEAFLQIVRLVVSAA"
     gene            273..995
                     /gene="secG"
                     /locus_tag="VGB85_00730"
     CDS             273..995
                     /gene="secG"
                     /locus_tag="VGB85_00730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEY0132559.1"
                     /translation="MFDILVTIITVFYVIICILMILIILLQAGRGGMGTALGGGASQS
                     VFGGGGSSDVLAKITQGCSAGFMIFAMFLAYASAHSGSANLKAKSEENSVEERAAGED
                     DVVNYERIGPNPLPLPARRDPATASTPTDAGQPAEPTEPAPIPEDAAPADPVAPADPA
                     APAEPAAADSAAAPADAAPAEGEPTAPADTKAADGKAADAPADTKPAKKATDAPAKKK
                     ANRPAPAPADAPTDAPVEAPAP"
     gene            992..1846
                     /gene="dapF"
                     /locus_tag="VGB85_00735"
     CDS             992..1846
                     /gene="dapF"
                     /locus_tag="VGB85_00735"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEY0132560.1"
                     /translation="MTSSPVLPARRTGLPFLKVEGLGNDFLLIDLRDRDPGALAEIVE
                     QLQQRAPALCDRRTGVGGDGLLLVTPPRRPGSTGTMLVINHDGSRPEMCGNGLRCVAL
                     HLADGPTTLQVDTDAGVRLCHVRPDASGQAGEVEVDMGHAEPLGPRTVGGRSFAAFSL
                     GNPHAVAFVAPGEDPEQLARTLGPRVETDPQFPARTNVEFARREADGSLTLWVWERGC
                     GITDACGTGACATLAAAVAEGLAPTGVPVRVQLPGGPLLVRLDDGRVWMTGPARVVFR
                     GEVLAGDR"
     gene            complement(1847..>2541)
                     /locus_tag="VGB85_00740"
     CDS             complement(1847..>2541)
                     /locus_tag="VGB85_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0132561.1"
                     /translation="GHPAFVVFGGMSSFRKDLLAGQVALVTGGGSGIGAGIAEALAAV
                     ITAIEARWGRLDVLVNAAAGNFLAPAATLSANGFGTVVDIDLKGTFNACKAALPLLER
                     NGGVVVNVSATLHYAGTPLQVHAASAKAGIDALTRTLAVEWGPAQIRVNAIAPGPIAG
                     TEGMERLAPPAMRAKMTAQIPLRRFGTVAEIGEAVLFMASPASAYMTGAVLVVDGGQW
                     LTGTNMQMLMSE"
BASE COUNT          353 a         1041 c          825 g          322 t
ORIGIN      
        1 cggtgtgggc gatcggcacc ggcaagaccg cgaccccgcg ggacgccgcc agcatgcacc
       61 gccgcctccg ggcctggctg gagcagcagt tcggcgccgc aggggccgac agatcgatcc
      121 tgtacggtgg tagtgtcaag cccaacaacg cggcggcgct gatggccgcc ggtgacgtcg
      181 acggcttcct cgtcggcggc gcctccctcg aggccgaagc cttcctccag atcgtccgcc
      241 tcgtcgtcag cgcggcctag cggcaagtcc ccatgtttga catcctcgtc accatcatca
      301 ccgtcttcta cgtgatcatc tgcatcctga tgatcctcat catcttgctg caggcgggcc
      361 gcggtggcat gggcacggcg ctcggcggag gcgcgagcca gagcgtcttc ggcggtggcg
      421 gtagctccga tgttcttgca aagatcacgc agggttgctc cgcgggcttc atgatcttcg
      481 caatgttcct cgcgtacgcc agcgcgcaca gcggctcggc caacctcaag gccaagagcg
      541 aggagaacag cgtcgaggag cgcgccgcgg gcgaggacga cgtcgtcaac tacgagcgca
      601 tcggacccaa cccgctcccg ctccccgccc gccgcgaccc ggccaccgcg tccaccccga
      661 ccgacgccgg tcagcccgcc gagccgaccg agcccgcgcc gatccccgag gacgccgccc
      721 cggccgaccc cgtagcgccg gctgaccccg ccgcccccgc cgagcccgcg gccgccgact
      781 ccgccgcagc tccggccgac gccgccccgg ccgagggcga gcccacggcg ccggccgaca
      841 ccaaggccgc cgacggcaag gccgccgacg cgcccgccga caccaagccg gccaagaagg
      901 cgaccgacgc ccccgcgaag aagaaagcca accgccccgc cccggctccc gccgacgccc
      961 ccaccgacgc ccctgtcgag gcgcccgccc cgtgacctcc tcgcctgtcc ttccggccag
     1021 gcgcaccggg ctgcccttcc tcaaggtcga gggcctcggc aacgacttcc tcctgatcga
     1081 cctgcgcgac cgcgacccgg gcgcgctcgc cgagatcgtc gaacagctcc agcagcgcgc
     1141 ccctgccctc tgcgaccggc gcaccggtgt cggcggcgac ggcctgctgc tggtcacccc
     1201 accccggcgc cccggctcga ccggcacgat gctggtcatc aatcatgacg gctcgcgccc
     1261 cgagatgtgc ggcaacggcc tgcgctgcgt ggccctccac ctcgccgacg gcccgaccac
     1321 gctgcaggtc gacaccgacg cgggcgtccg cctgtgccac gtgcgacccg acgcgagcgg
     1381 ccaggccggc gaggtcgagg tcgacatggg ccacgccgag ccgctcggcc cacgcacggt
     1441 cggcggtcgc agcttcgccg ccttctcgct cggcaacccg cacgcggtcg ccttcgtcgc
     1501 tcccggtgag gaccccgagc agctcgcccg caccctcggc ccccgcgtcg agaccgaccc
     1561 gcagttcccc gcccgcacca acgtcgagtt tgcccgccgc gaagccgacg gcagcctgac
     1621 gctgtgggtc tgggagcgcg gctgcggcat cacggacgcc tgcggcaccg gagcctgcgc
     1681 cacgctcgcg gcggcggtcg ccgaggggct cgcgccgacc ggcgtgccgg tgcgcgtgca
     1741 gctcccgggt ggcccgctgc tcgtccgcct cgacgacggc cgcgtctgga tgaccgggcc
     1801 ggcgcgcgtc gtgtttcgcg gcgaggtcct cgcgggcgac cgctgactac tcgctcatca
     1861 acatctgcat gttggtcccg gtcagccact gcccgccgtc gaccacgagc accgcccccg
     1921 tcatgtaggc gctcgccggg ctcgccatga acagcaccgc ctccccgatc tccgcgaccg
     1981 tgccgaagcg ccgcagcggg atctgggccg tcatcttcgc gcgcatcgcc ggtggggcca
     2041 ggcgctccat gccctcggtg ccggcgatcg gtcccggcgc gatcgcgttg acgcggatct
     2101 gcgccggccc ccactcgacc gcgagcgtcc gcgtcagcgc gtcgatgccc gccttcgcgc
     2161 tcgccgcgtg cacctgcaac ggcgtgcccg cgtagtgcag cgtcgccgac acgttgacca
     2221 ccacgccgcc gttgcgctcg agcagcggca gcgcggcctt gcaggcgttg aacgtgccct
     2281 tgagatcgat gtcgacgacg gtgccgaagc cgttggccga cagcgtcgcc gccggcgcga
     2341 ggaagttgcc ggccgccgcg ttgaccagca catcgagtcg accccagcgc gcctcgatcg
     2401 cggtgatcac cgccgcgagc gcctcggcga tgcccgcacc gatccccgag ccgccgccgg
     2461 tcacgagcgc cacctgaccg gcgaggaggt ccttacgaaa gctgctcatg cccccaaata
     2521 ccacgaacgc cggatgacca c
//
LOCUS       DASWNJ010000038         3201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_671876, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000038 DASWNJ010000000
VERSION     DASWNJ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671876"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="VGB85_00745"
     CDS             complement(<1..1202)
                     /locus_tag="VGB85_00745"
                     /inference="COORDINATES: protein motif:HMM:NF018827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132562.1"
                     /translation="MSTASRAESSAIMDMLGVAENVAGTIDPASLLRALFAAGRGVVR
                     HPIDTSAAVGRAAVGAGSAWWAAAGRMVGRKSAGPVRAGAGDRRFADPAFEDNPVYFW
                     LAQNHLLAERYVSELIDAAALGEAEDRKARFVTRFLLDAAAPTNTLLGNPQALRAAFD
                     SGGKSVVRGLGNWVKDLRHNGGWPKQVDASGFKVGANMASTPGKVVHRTDLIEVLQYQ
                     PQTADVHEIPLLFCPPWINKYYILDLAPGKSLIEWAVRHGHTCFTISYRNPDAAMRDV
                     SFEDYLMKGPLDAMAVIREITGSPVVNTVSVCLGGTLSAIGLAYEAREGQKSVNAATF
                     LNAHTDFEIQGILGTFTDGETFAALEKKMAKVGYLDARDMARTFDVLRANDLIFSYVV
                     NNWLLGKDP"
     gene            1361..2221
                     /locus_tag="VGB85_00750"
     CDS             1361..2221
                     /locus_tag="VGB85_00750"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0132563.1"
                     /translation="MTQLVWDRAGAGAPLLLLHGIGSTHDDFIAVRAALESEYEVLAV
                     DLPGHGGSPPMSRRPTVSAIADILEADLDMLGFSRVHILGNSLGGRLALELARRDRAL
                     SVVAIAPSGLNIIPERIYQGTALSMTRLMLRTFRPVIAPLARFRLTRAGMLAGLRARP
                     WRASEREVLAMRDGLGDSEGFWSMLFWSTLVDIPRGLRNVRCPVILAQGNADTVALGQ
                     TPRYLLLVPGATFKPLWFAGHAPMSDVPAAIVRLVEEATRRAGPPSTQPPSTQPPSTQ
                     ASPLGPSAPG"
     gene            2351..>3201
                     /locus_tag="VGB85_00755"
     CDS             2351..>3201
                     /locus_tag="VGB85_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0132564.1"
                     /translation="MALGAMGVAAAMLARRARRIDLRDKVVVVTGGGRGLGFAVAREL
                     RRRGCRLAICGRDGEVVARAAEALGGDVFAAACDASDPAQVGEFVAAVLRRFGRIDVL
                     INNAGQCFVGPIVELQQADLEYALRNIFWAHVHPTMAIVPHMRARGSGRIVNVTSMGG
                     KVPIPHQAAYVASKYAATGWSEVLTIELHKEGILVSTVTPPPLDDGAPVHAHFHGRED
                     GEFRWFASMLSSPLTATRTERAARAIVDAAEYGDAARAISLDSWLGARTHGLAPGLVS
                     RLLARVER"
BASE COUNT          443 a         1174 c         1090 g          494 t
ORIGIN      
        1 gggtccttgc cgagcagcca gttgttgacc acgtacgaga agatcaggtc gttggcgcgc
       61 agcacgtcga aggtgcgggc catgtcgcgg gcgtcgaggt agccgacctt ggccatcttc
      121 ttctcgagcg cggcgaaggt ctcgccgtcg gtgaaagtcc cgaggatgcc ctggatctcg
      181 aagtcggtgt gggcattgag gaaggtcgcc gcgttgaccg acttttggcc ctcacgcgcc
      241 tcgtacgcga ggccgatcgc cgagagggtc ccgccgaggc acaccgatac ggtgttgacc
      301 accggcgatc ccgtgatctc ccggatcacc gccatcgcgt cgagcgggcc cttcatcagg
      361 taatcctcga acgacacgtc gcgcatcgcg gcgtcggggt tgcggtaact gatggtgaag
      421 caggtgtgcc cgtggcgcac cgcccactcg atcaggctct tcccgggggc cagatccaaa
      481 atatagtact tgttgatcca cggcgggcag aacagcagcg ggatctcgtg cacgtcagcg
      541 gtctgcggct gatattgcag cacctcgatc aggtcggtgc gatgcacgac cttgcccggc
      601 gtcgacgcca tgttggcgcc gaccttgaaa cccgaggcgt cgacctgctt cggccagccg
      661 ccgttatggc gcaggtcctt gacccaattg ccgagcccgc gcacgacgct cttgccgccg
      721 ctgtcgaacg cggcgcgcag cgcctgcggg ttgccgagca gcgtgttggt cggcgccgcc
      781 gcgtcgagca ggaacctggt gacgaagcgg gccttgcggt cctcggcctc cccgagcgcc
      841 gccgcgtcga tcagctccga gacgtagcgt tcggccagca gatggttctg cgccagccag
      901 aaatacaccg ggttgtcctc gaaggccggg tccgcgaagc ggcggtcgcc ggccccggcc
      961 cgcacgggcc cggcgctctt gcggccgacc atgcgcccgg cggccgccca ccaggccgag
     1021 cccgccccga ccgccgcccg gccgacggcc gcgctcgtgt cgatcgggtg acgcacgaca
     1081 ccacggccgg cggcgaacag cgcgcgcagc aacgacgccg gatcgatcgt gccggcgacg
     1141 ttctcggcga cgccgagcat gtccatgatg gccgaagatt cggcgcgcga ggcggtcgac
     1201 atggctggct tttgcgtgaa gtgtcgcggc caggcaaatg tgagccgcgc atggtcaggt
     1261 gtccgccgcg acgcacggac gcgcgacccg ggacattcct gacgtggcgc agaacaccgg
     1321 ctgggtcgca caattcccgt ttcgagggta ctaacaggcg atgacgcagc tcgtctggga
     1381 ccgcgccggg gccggcgctc cactcctcct cttacacggc atcggcagca cacacgacga
     1441 tttcatcgca gtacgcgccg cgctggagtc cgagtacgag gtgctcgccg tcgatcttcc
     1501 gggccacggc ggatcaccgc cgatgtcgcg tcggccgacc gtcagcgcga tcgccgatat
     1561 cctcgaggcc gacctcgata tgctcgggtt ctcccgggtc catatcctcg gcaattccct
     1621 cggtggccgg ctcgcgctgg agctggcgcg ccgcgaccgg gccctctcgg tcgtggcgat
     1681 cgcgccgtcg ggcctcaata tcatcccgga gcggatctat cagggtaccg cgctgagcat
     1741 gacacgattg atgttacgta cattccggcc ggtgatcgcc ccgctcgccc gctttcgcct
     1801 gacccgcgcg ggcatgctgg ccgggctgcg cgcgcggccg tggcgggcct ccgagcgcga
     1861 ggtgctggcg atgcgcgacg ggctcggcga ctccgagggc ttctggtcga tgctgttctg
     1921 gtcgaccctc gtcgacatcc cgcgcggact ccggaacgtg cgctgcccgg tgatcctcgc
     1981 ccagggcaac gcggacaccg tcgccctcgg ccaaaccccg cgctacctgc tcctcgtacc
     2041 cggcgcgacc ttcaagccgc tgtggttcgc ggggcatgcg ccgatgtccg acgtacccgc
     2101 cgcgatcgtg cgcctcgtcg aggaggcgac ccggcgcgcc ggaccaccgt cgacgcaacc
     2161 accgtcgacc caaccaccgt cgacgcaagc atcaccgctc ggcccgtccg cccccggcta
     2221 aaagcgtgcc gcctgcgcat tccggccgcc ccgggccgtg cgcgggccgt gcacgccgcg
     2281 ctggcccgcg ccgatcgaca ggcgccccgg gtcgcgcacc ctggggccca tgcacgcgat
     2341 cgttcgaacg ttggcgctcg gcgccatggg tgttgcggcg gcgatgctcg ctcgccgggc
     2401 gcggcggatc gacctgcgcg acaaggtcgt cgtcgtcacc ggcggcggtc gtggcctcgg
     2461 cttcgcggtc gcccgcgagc tgcgccgccg cggctgtcgc ctggcgatct gcgggcgcga
     2521 cggcgaggtc gtcgcccggg cggccgaggc cctcggcggc gacgtgttcg ccgcggcctg
     2581 tgacgccagc gacccggcgc aggtcggcga attcgtcgcg gcggtcttgc gccgcttcgg
     2641 gcgcatcgac gtgctgatca acaacgccgg gcagtgcttc gtcgggccga tcgtcgagct
     2701 gcagcaggcc gacctcgagt acgcgctgcg caacattttt tgggcccacg tgcacccgac
     2761 gatggcgatc gtcccgcaca tgcgcgcgcg cggctccggc cggatcgtca acgtgacctc
     2821 gatgggcggc aaggtcccga tcccgcacca ggccgcctac gtcgccagca aatacgccgc
     2881 caccggctgg tccgaggtcc tgacgatcga gctgcacaag gagggcatcc tcgtcagcac
     2941 cgtcaccccc ccgccgctcg acgacggcgc ccccgtgcac gcccacttcc acggccgcga
     3001 ggacggcgag tttcgctggt tcgcaagcat gctgtcctcg ccgctgaccg cgacccgcac
     3061 cgagcgggcc gcccgggcga tcgtcgacgc ggccgagtac ggcgacgccg cgcgggcgat
     3121 ctcgctcgac tcctggctcg gcgcccgcac ccatggcctc gcgccgggcc tcgtctcccg
     3181 cctgctggcc cgcgtcgaac g
//
LOCUS       DASWNJ010000039         3520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_283577, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000039 DASWNJ010000000
VERSION     DASWNJ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283577"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..997)
                     /locus_tag="VGB85_00760"
     CDS             complement(107..997)
                     /locus_tag="VGB85_00760"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0132565.1"
                     /translation="MRLRRDDGASLRLFSYGPRDAPGPAILILDGIGCAGWAFERSLP
                     TLSENRRVVLMHYRGHGQSPDPPKPWKLSIPVLADDAAAALEHLGIASAVVVGFSMGF
                     PVALELYRRHRHLVAGLVSLAGPSGRVLTSFQGTQAFGRLLPLVRATTRIAHDLTNRV
                     WRKVLPSPLSREISLRSGQVNADRIEAADLELYMRGIAAMNPELFLTLLDEAHRHSAA
                     DVLPTIAVPTLVVAGARDNFVPLATMRALAFSIPRVQWKVYADATHALPAEYPLEIAR
                     EILDFVDGTVAPFLEGSLST"
     gene            complement(1232..1441)
                     /locus_tag="VGB85_00765"
     CDS             complement(1232..1441)
                     /locus_tag="VGB85_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04563 family protein"
                     /protein_id="HEY0132566.1"
                     /translation="MASSDKRKQSLYFPEQMLKEIQEEAARQDRSLSWIVQKAWKIAR
                     KDIMKYPSVNDLADDGADDERGRNE"
     gene            1498..2532
                     /locus_tag="VGB85_00770"
     CDS             1498..2532
                     /locus_tag="VGB85_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0132567.1"
                     /translation="MAVNRDREAFVAAEGAADTGSGELGSSGLPASGAEARGVAASAG
                     GVGEESDGRAVRAQVRREQRRSTILVAATRVFRDKGYHAASVNDIIDAAQIARGTFYL
                     YFTSKREIFAELTATFLGLIRGSVQKISLDPEAGEPLAQMRANFRRVMNTVLEHGDLA
                     TIMLRGDSGDAEARAQLDLFYRQVVALIGQAVTVGHALGFARDCDVHIIAVAALAGLK
                     EVLGRMLDARQAADATAAEALHAPAAVDADVDADVDVDADVAVVADPARSTDATGVSG
                     PTRDGAGRGHGDTAAPTQRSGVMVGDAGENAGGNDRSGVQSPWLMPSERLADELLTFF
                     VRGVFTEDAR"
     gene            2615..2983
                     /locus_tag="VGB85_00775"
     CDS             2615..2983
                     /locus_tag="VGB85_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEY0132568.1"
                     /translation="MTEAQMKKFRAILEERRTSLIVQAKKTLESDMVLSPDDRFDEVD
                     QASSEYMQAFSFRLRGREKFLMDKIELALRKIDEGTYGVCEECEETIALKRLQARPEA
                     PLCIQCKEAQEKEEAVYAEE"
     gene            3084..>3520
                     /locus_tag="VGB85_00780"
     CDS             3084..>3520
                     /locus_tag="VGB85_00780"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEY0132569.1"
                     /translation="MHPQLFTVPGTEFVVGAYGLCMGLALVFAWVTALWLARKDGLPS
                     DRLGTVFVLAAIAGLLCARAVWLLQQGQPLGLAELRSLPAGGMTVFGGAFAALLVCVI
                     GARRVGVPLLAWLDCVAPAFALGAISERIGALLAGGDFGRYVGV"
BASE COUNT          512 a         1061 c         1381 g          566 t
ORIGIN      
        1 cggagacgcg cgcgcgacac atcgagcgct tcatcgccat gctcggcaag ggcgagacga
       61 tccacccgcc gcgcaagaag ccctgaggcc cgcgaggtcg ggccgatcag gtcgagagcg
      121 acccctcgag gaatggggcg acggtcccgt cgacgaagtc gaggatctcg cgcgcgatct
      181 cgagggggta ctcggcaggc agggcgtggg tcgcgtcggc gtagaccttc cactggacgc
      241 gggggatcga gaaggcgagg gcgcgcatgg tggcgagcgg gacgaagttg tcgcgggcgc
      301 cggcgaccac gagggtgggg acggcgatcg tcggcagcac gtcggcggcg ctgtggcggt
      361 gggcctcgtc cagcagggtc aggaacagct ccgggttcat cgccgcgatg ccgcgcatgt
      421 agagctcgag gtcggcggcc tcgatgcggt cggcgttgac ctggccggag cgcaggctga
      481 tctcgcggga cagcggggag ggcaggacct tgcgccacac gcggttggtc aggtcgtggg
      541 cgatgcgggt ggtcgcgcgg accagcggga gcaggcggcc aaaggcctgg gtgccctgga
      601 agctggtcag cacgcggccg ctggggccgg cgagcgagac caggccggcg accaggtggc
      661 ggtgacgacg gtagagctcg agggcgacgg ggaagcccat cgagaagccg acgacgaccg
      721 cggaggcgat gccgaggtgc tcgagggccg ccgcggcgtc gtccgccagg accgggatgc
      781 tgagcttcca gggtttgggc gggtcggggg actggccgtg gccgcggtag tgcatgagca
      841 cgacgcggcg gttctcggac agcgtgggca ggctgcgctc gaaggcccag ccggcgcagc
      901 cgatgccgtc gaggatgagg atcgcggggc cgggggcgtc gcgggggccg tagctgaaca
      961 ggcgcagaga cgcgccgtcg tcgcgacgca ggcgcactgg tgggacgctg tttcggaagc
     1021 ccgggtcccc gtggccggcg tggcgtgggg gggtagggct ggcagggcgg ccggggtcag
     1081 cggagtgcat ggcgtcgtgc gccgtggggt gtgtcgcgga tggtgacgat cgccgtggcc
     1141 cgcaggccac agcgtacgct cgcccccggg ggagtcgcag gtgtaccggg agggccagga
     1201 gcgcgcgggg gcaccggtgc gctccgggag ctcactcgtt acgaccgcgc tcgtcgtcgg
     1261 cgccgtcgtc cgccaggtcg ttgacgctgg ggtacttcat gatgtccttg cgggcgatct
     1321 tccacgcctt ttggacgatc catgacagcg agcggtcctg gcgcgcagcc tcctcctgga
     1381 tctccttgag catttgttcc gggaaataga gcgactgctt gcgcttgtcc gagctggcca
     1441 ttctggtgtg gtctccgcta gtcgtaatcg cggtttttag ggtaggctgc gcatggattg
     1501 gctgtcaacc gcgaccgtga agcctttgtc gccgccgagg gggccgcaga caccggctcc
     1561 ggggagctgg ggtcctctgg tttaccggcg agtggagcgg aggcgcgcgg tgtcgcagcg
     1621 agcgccgggg gtgtcggaga ggagtccgac ggccgggcgg ttcgcgcgca ggtgcgtcgt
     1681 gaacagcggc gcagcaccat cctcgtggcc gccacgcggg tcttccggga caagggttat
     1741 cacgctgcgt cggtgaacga catcatcgac gcggctcaga tcgcgcgcgg gaccttctat
     1801 ctctacttta cgagcaaacg tgagatattt gccgagctga cggcgacctt cctcgggctg
     1861 atccggggca gcgtacaaaa gatctccctc gatcctgaag ctggcgagcc gctcgcacaa
     1921 atgcgggcca actttcgccg ggtgatgaac accgtgctcg agcacgggga tctggcgacg
     1981 atcatgttgc gcggcgactc gggggacgcc gaggcgcggg cgcagctgga tctcttctat
     2041 cgccaggtcg tcgcgttgat aggccaggcg gtgacggtcg ggcacgctct cggcttcgcg
     2101 cgcgactgcg acgtgcacat catcgccgtg gcggccctgg caggcctcaa ggaggtgctc
     2161 gggcgcatgc tggacgcgcg tcaggccgcg gacgcgaccg cggcggaggc attgcacgcg
     2221 cccgcggccg tagatgccga cgtagatgcc gacgtagatg tcgatgccga tgtagctgtc
     2281 gtggctgatc ctgcgcgctc gacggatgcc acgggggtct ccgggccaac tcgcgatggc
     2341 gccgggcgag ggcacggcga caccgcggct cccacgcagc gaagcggtgt catggtcggc
     2401 gacgccggtg aaaatgctgg aggcaacgac cggagcggtg ttcagtcacc ttggctgatg
     2461 ccctctgagc gcctcgcaga cgagcttttg accttctttg tgcgtggcgt gtttaccgaa
     2521 gacgccaggt gagctagtgt gtcgcgccct tggagacccc ggcgctgtgt taccttcgag
     2581 ggccgaggac aggaccggag cggcactcat ggcgttgacc gaagctcaga tgaagaaatt
     2641 tcgtgcgatc ctcgaagagc ggcgtacgtc gttgatcgtg caggccaaaa agacgctcga
     2701 gagcgacatg gtcctgtcgc ccgacgaccg cttcgacgag gtcgatcaag cctcgagcga
     2761 gtacatgcag gccttcagct tccgcctccg cgggcgcgag aagttcctga tggacaagat
     2821 cgagctggcc ctgcgtaaga tcgacgaggg cacctacggc gtgtgcgagg agtgcgagga
     2881 gacgatcgcc ctcaagcgcc tgcaggcgcg cccggaggcc ccactgtgca tccagtgcaa
     2941 ggaggcccag gagaaggaag aggcggtcta cgccgaggag tgatgcgggc cgcgtgaccg
     3001 gcctggcgct tcgccgggcc gcgtgacggg gctgccccac gtgctgccgg tggctcgtta
     3061 gcccccgtcg gttgggtcga tcgatgcacc cgcaattgtt caccgtgccg ggcacggaat
     3121 tcgtggtcgg cgcgtacggc ctgtgcatgg gactcgcgct ggtgttcgcg tgggtgaccg
     3181 cgctgtggct cgcccgcaag gacggcctgc cgagcgaccg gctcggcacc gtgttcgtgc
     3241 tcgccgcgat cgcggggttg ctgtgcgcgc gggcggtgtg gttgctgcag cagggccagc
     3301 cgctcgggct cgccgagctg cgcagcttgc cggcgggcgg catgaccgta ttcggcgggg
     3361 ccttcgcggc gctgctggta tgcgtgatcg gtgcgcggcg ggtcggcgtg ccgttgctgg
     3421 cctggctcga ctgcgtcgcg ccggccttcg cgctcggggc gatcagcgag cgcatcgggg
     3481 cgttgctggc cggtggcgac ttcgggcgct acgtcggggt
//
LOCUS       DASWNJ010000040         2514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_354142, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000040 DASWNJ010000000
VERSION     DASWNJ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354142"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..>2514)
                     /locus_tag="VGB85_00785"
     CDS             complement(168..>2514)
                     /locus_tag="VGB85_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0132570.1"
                     /translation="DLDGDGRPELVVAAGPWKAYDLRVLRGGSDGTLTLAARRTLGTI
                     HALALLRTASGPVIALIKNDEYPNPRRFSRAHPSGPPAGLYLIGLRGDALEILGFAPA
                     QHGDAPRQEMRRLSAGDLDGDGLDELIAADRDGAATLLRWQPGADPRPLVFPGLDPLL
                     VLDLDGDGRSEIVAAPADDPTRVLVLGAGERVLSPAQRVLPAPRSPPAALTDPVAVAA
                     WIRGEELVTIGLPRRTAAELAALAGLSGHGASDMFLRSAELHAGLGEHARAAELFLAA
                     ATRPEHAAAALGGAAESRRRLGEFATAAALTAARLRMPGQTPAALATIRRELTALQTA
                     IAPRPQQTLDFALPLAQSWRIDDPLAVGRDLARQALSLWSSPAPTIAELPLLWDGGPA
                     TLEVELEVDRLEWGAELGVGIVGEGRDRMWIGARVGAYGANDDPVTTLSLFDDFRQKH
                     HPTPVRAGTRIIARIAVFPAQGTRIDEVEIDGVLQHRRVQASAHGPDSAEPTPGPVRL
                     RFTGVPVEADLVAHVWIRRIELVGFAPAAARPDPVPDPARLLAERDLVAALAALDPAH
                     PLARVWRAEALAALGQVEPASAELRAFVADHPDDDPLYQHLHHRLRRGGDTLALAARS
                     GLGDRHVDIVRGLLGRQPRDRDIADALVFWPEIPTDVPADSRGRARRVDLLYLRGLAL
                     LRAGRHDAAARSYAAAMTLLDDPARPVDDAASRREQLLLARAYLSAETGDIAATLTAI
                     HTLLGESSTPALLLESLQADPALTSLLTADHWHALGAAPRE"
BASE COUNT          320 a          857 c         1032 g          305 t
ORIGIN      
        1 ccgcggccgg ccgcacctga aggtttgcgg aatgcatggg cgcgaacctt aagcacgccc
       61 gcggtcgcgt aaagaggtcg cgcgcggcgc cgaaggaatt acttcacccc ggaatgattg
      121 tggtttgagc gtgagcgcct cgatcatgcc acgtggcatc ggcgtcgtca ctcacggggc
      181 gcagcgccga gggcgtgcca gtggtccgcg gtgagcaggc tcgtgagcgc ggggtcggcc
      241 tgaagggact cgagcagcag cgccggcgtc gagctctcgc ccaggagcgt gtggatcgcg
      301 gtcagcgtgg cggcgatatc acctgtctct gcggacaggt aggcgcgcgc gagcagcaat
      361 tgttcgcgcc gcgacgccgc gtcgtcgacg ggccgggccg ggtcgtcgag cagcgtcatc
      421 gcggcggcgt agctgcgcgc ggcggcgtcg tggcggccgg cgcggagcag ggcgaggccg
      481 cgcaggtaca gcagatcgac ccggcgcgcg cgcccccggg agtcggcggg cacatcggtc
      541 gggatctcgg gccagaacac gagcgcgtcg gcgatgtcgc ggtcgcgcgg ctggcgtccg
      601 agcaggccgc ggacgatgtc gacgtggcga tcgccgaggc cgctgcgcgc cgcgagggcg
      661 agcgtgtcgc cgccgcggcg caggcgatgg tggaggtgct gatacagggg gtcgtcgtcg
      721 gggtggtcgg cgacgaaggc ccgcagctcg gcgctcgccg gctcgacctg gccgagcgcg
      781 gccagggcct cggcgcgcca cacccgggcc agcgggtgcg cggggtcgag ggccgcgagc
      841 gcagcgacca ggtcgcgctc cgcgagcagg cgggccgggt cgggcaccgg gtcgggccgg
      901 gcggccgcgg gcgcgaagcc gacgagctcg atgcgacgga tccacacgtg ggcgacgagg
      961 tctgcctcga ccgggacacc ggtgaatcgc aggcgcaccg ggccgggggt cggctcggcg
     1021 gagtcgggcc cgtgcgcgct ggcctggacg cgacgatgct gcagcactcc gtcgatctcc
     1081 acctcgtcga tcctggtgcc ctgggccggg aatactgcga tgcgcgcgat gatcctcgtg
     1141 cccgcccgaa ccggggtcgg gtggtgtttc tggcgaaagt catcgaacag cgacagcgtg
     1201 gtcacgggat cgtcgttggc tccgtaggcg ccgacacggg cgccgatcca catgcggtcg
     1261 cggccctcgc cgacgatccc gacgccgagc tcggcgcccc actcgaggcg atcgacctcg
     1321 agctcgacct cgagggtcgc cgggccgccg tcccacagca gcggcagctc ggcgatcgtc
     1381 ggcgcgggcg aggaccacag ggacagtgcc tgtcgcgcca ggtcgcgccc gaccgccagc
     1441 gggtcgtcga tccgccagct ttgcgcgagc ggcagcgcga agtcgagggt ctgctgcggc
     1501 cgcggggcga tcgccgtctg caacgcggtc agttcgcggc ggatcgttgc cagggccgcg
     1561 ggcgtctggc ctggcatgcg caggcgggcg gcggtcaggg ccgcggcggt cgcgaattca
     1621 ccgagtcggc ggcggctctc ggcggccccg cccagagcag cggccgcgtg ctcgggtcgg
     1681 gtcgccgcgg cgaggaacag ctcggccgcg cgggcgtgct cgccgaggcc ggcgtgcagc
     1741 tcggcggacc gcaagaacat gtccgaagcg ccatgtcccg agagaccggc gagcgccgcg
     1801 agctcggcgg cggtgcggcg cggcaggccg atggtgacca gctcctcgcc gcgtatccag
     1861 gccgcgacgg cgaccgggtc ggtcagggcc gcaggaggtg atcggggcgc cggcaggacg
     1921 cgctgtgccg gggacaggac gcgctcgccg gcgccgagca cgagcacgcg cgtgggatcg
     1981 tcggcggggg cggcgacgat ctcgctgcgc ccgtcgccgt cgaggtcgag cacgagcagg
     2041 gggtcgaggc ccgggaacac gagcgggcgc gggtcggcgc cgggctgcca gcgcagcagg
     2101 gtcgccgcgc cgtcgcggtc ggccgcgatc agctcatcga ggccgtcgcc gtcgaggtcg
     2161 ccggcgctca gcctgcgcat ctcctgccgc ggggcgtcgc cgtgctgcgc gggcgcgaag
     2221 ccgaggatct cgagcgcgtc gccacgcagg ccgatcaggt acaggcccgc gggcggcccg
     2281 gacgggtgcg cccgcgagaa ccggcgcggg ttggggtact cgtcgttctt gatcaatgcg
     2341 atcacggggc cgctcgcggt ccgcagcagc gcgagcgcgt ggatcgtgcc gagggtgcgg
     2401 cgcgccgcga gcgtgagcgt gccgtcgctg ccgccacgga gcacgcgcag gtcataggcc
     2461 ttccacggtc cggcggccac gaccagctcc gggcggccgt cgccgtcgag gtcg
//
LOCUS       DASWNJ010000041         3362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_601278, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000041 DASWNJ010000000
VERSION     DASWNJ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601278"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1448
                     /locus_tag="VGB85_00790"
     CDS             <1..1448
                     /locus_tag="VGB85_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0132571.1"
                     /translation="PNDRFDGHREATSLKLRWRPREHHEFQVISFYSHSFRRSVMATN
                     EDRNVGMDVTQLAVQPRLFNVFGVEPRYAFRVRHKRMFQDLSFGVRGVFEWARLREVW
                     SRDPNLPATNLGDTRSCERGVPYDRLGRRCLDGRTAGFSLYAEDKLYLLDTRLVLTAG
                     VRGEIMQQGFYNLLTRQSVPRPVQGGVLPGVSLWYGIDEVAGFLGYGRSFGAPSYFSA
                     TVTMPINDDSRYIQPEFADMVEGGVKLMELGGVYATFDVWYKYFRYLRDEGDNAISII
                     PAAHGYGVEAEVEWLPGEVWERVDGLELTAGYAYTGSNVRGDNYIGNRMPWYPVHEAW
                     GRASYRFPFGLKLGSSLDYTGEQFTDYGNIREWASGEIGIMPAYTLMSANVGFATPLP
                     QGWRLEFTVGAKNLLNEVWFTRTDDLNGGILAMRPRTFYFNLGIAHEFIRGRAAEQAR
                     SKGPGKPDRRRWTATENRRQRQMMRMIGAWI"
     gene            1445..1933
                     /locus_tag="VGB85_00795"
     CDS             1445..1933
                     /locus_tag="VGB85_00795"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEY0132572.1"
                     /translation="MIGRRTYFKVHQAIGVGAAAVLLLAALTGMLLVFRGYFKGPRPP
                     QAPVVEQPLALEELMARAVAAGPGDPVTDIGLPSAADRPYQFYLDDDDETIVYLAGDG
                     TVLEARKTAGGFTRTVFLLHTGEIAGKPGEVLNFLGGAAMTVLVVSGLMMIVSRRRAR
                     RM"
     gene            1949..2857
                     /locus_tag="VGB85_00800"
     CDS             1949..2857
                     /locus_tag="VGB85_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132573.1"
                     /translation="MHLILQEPEIDGVSMLIGIDSVIVQRRTQAGQELLIAETSAYGR
                     TLFLDGLVQSAEADEALYHEPLIHPALVLHGAPRRVLVGGTGEGASLREILRHASVER
                     IVSVDLDPEVVALCKEHLPSWSAGSLDDPRVELRVEDIQTTLASAQPGEYDAIFLDIT
                     DPVEDGPSAALFSTKFFTQVARALADDGIAVLQSGELDPIDLRMSRAVRSTLQAVFPF
                     VEFAHTYVPSFHCLWGFAIVAKRQIPREPADLAERIARLGELRVYTPTSHRAMLELPP
                     YLQRRLEQPGPVITGEGDLLSYTTGS"
     gene            2864..>3362
                     /locus_tag="VGB85_00805"
     CDS             2864..>3362
                     /locus_tag="VGB85_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132574.1"
                     /translation="MWSRTNLLIVAAAERLGARAEALGQRHTDFLLRLVHGERRVMIS
                     KTRSPFLTQVAQTLANNKHLSRALLRGHGVPCVPDLLLDETDDDAAAAGMLATHGRVV
                     VKPNWGNRGVGVTTDVRTMAVLRRARARARAIDLDEEVVIEPFVAGTNLRVAVVGGRC
                     VAACEV"
BASE COUNT          498 a         1127 c         1232 g          505 t
ORIGIN      
        1 gcccgaacga ccgcttcgac ggccaccgcg aggcgaccag cctcaagctg cgctggcggc
       61 cgcgcgagca tcacgagttt caggtgatct cgttctacag ccacagcttt cgccgcagcg
      121 tgatggcgac caacgaggac cgcaacgtcg ggatggacgt cacgcagctg gcggtccagc
      181 cgcgcctgtt caacgtgttc ggggtcgagc cgcgctacgc gtttcgcgtg cgccacaagc
      241 ggatgttcca ggacctcagc ttcggggtgc gcggggtgtt cgagtgggcg cgcctgcgcg
      301 aggtctggtc gcgcgacccc aacctgccgg cgaccaacct cggcgacacc cgcagctgcg
      361 agcgcggggt gccgtacgac cgcctcggcc ggcgctgcct cgacgggcgc acggccggct
      421 tctcgctgta cgccgaggac aagctgtacc tgctcgacac gcgcctggtg ctgaccgcgg
      481 gcgtgcgcgg ggagatcatg cagcagggct tttataacct gctcacgcgc cagtcggtgc
      541 cgcggccggt gcagggcggc gtgctgccgg gcgtgtcgct gtggtacggg atcgacgagg
      601 tcgccggctt cctcggctac gggcgatcgt tcggcgcgcc gagctacttc tcggcgacgg
      661 tcaccatgcc gatcaacgac gacagccgct acatccagcc ggaattcgcc gatatggtcg
      721 agggcggcgt caagctgatg gagctcggcg gcgtgtatgc gaccttcgac gtctggtaca
      781 agtattttcg ctacctgcgc gacgagggcg acaacgcgat ctcgatcatc ccggcggcgc
      841 acggctacgg cgtcgaggcg gaggtcgagt ggctgcccgg cgaggtgtgg gagcgcgtcg
      901 acggcctcga gctgaccgcc ggctacgcgt acaccggcag caacgtgcgc ggcgacaatt
      961 acatcggcaa ccgcatgccc tggtacccgg tgcacgaggc gtggggccgc gcgtcgtatc
     1021 gcttcccgtt cgggctgaag ctcggcagct cgctcgacta caccggggag cagttcacgg
     1081 actacggcaa catccgcgag tgggcgagcg gcgagatcgg gatcatgccg gcctacacgc
     1141 tgatgagcgc gaacgtcggc ttcgcgaccc cgctgccgca gggctggcgg ctcgagttca
     1201 cggtcggcgc caagaacctg ctcaacgagg tatggttcac gcgcaccgac gacctcaacg
     1261 gtggcatttt ggcgatgcgg ccgcgcacct tctacttcaa cctcggcatc gcccacgagt
     1321 tcatccgcgg ccgcgccgcg gagcaggcgc gcagcaaggg cccgggcaag ccggaccggc
     1381 gccgctggac ggcgaccgag aaccgcagac agcgccagat gatgcgcatg atcggggcct
     1441 ggatatgatc ggacgcagga cctacttcaa agtccaccag gcgatcggcg tcggcgcggc
     1501 ggcggtgctg ttgctggcgg cgctgaccgg catgctgctg gtgtttcgcg gctacttcaa
     1561 aggtccgcgc ccgccgcagg cgccggtggt cgagcagccg ctcgcgctcg aggagctgat
     1621 ggcgcgcgcg gtcgcggcgg gccccggaga cccggtgacg gacatcggct tgccgtccgc
     1681 ggccgaccgg ccctaccagt tctacctcga cgacgacgac gagacgatcg tgtacctcgc
     1741 gggcgacggc accgtgctcg aggcgcgcaa gacggccggc ggcttcacgc gcacggtgtt
     1801 cctcctgcac accggcgaga tcgccgggaa gccgggcgag gtcctcaact tcctcggcgg
     1861 cgccgcgatg acggtgctgg tggtcagcgg actgatgatg atcgtgtcgc gccgtcgcgc
     1921 gcggcgcatg tgagcggagg atcgacgtat gcacctcatt ttgcaggaac ccgagatcga
     1981 cggcgtgtcg atgctgatcg gcatcgacag cgtgatcgtg cagcgccgca cgcaggcagg
     2041 tcaggagctg ctgatcgccg agacctcggc ctacgggcgc acgctgttcc tcgacggcct
     2101 cgtgcagtcg gccgaggccg acgaggcgct gtaccacgag ccgctgatcc acccggcgct
     2161 ggtattgcac ggcgcgccgc gccgggtgct cgtcggcggc accggggagg gcgcgagcct
     2221 gcgcgagatc ctgcgccacg cgagcgtcga gcggatcgtc agcgtcgacc tcgatcccga
     2281 ggtggtggcg ctgtgcaagg agcacctgcc gtcgtggagc gccggcagcc tcgacgaccc
     2341 gcgcgtcgag ctgcgcgtcg aggacatcca gacgacgctg gcgagcgcgc agcccggcga
     2401 gtacgacgcg atcttcctcg acatcaccga cccggtcgag gacgggccgt cggcggcgct
     2461 gttctcgacg aagttcttta cgcaggtggc ccgcgcgctg gccgacgacg gcatcgcggt
     2521 gctgcaatcc ggtgagctcg acccgatcga cctgcggatg tcgcgggcgg tccgcagcac
     2581 gctgcaggcg gtgttcccct tcgtcgagtt cgcgcacacc tacgtgccgt cgtttcattg
     2641 tctgtggggg ttcgcgatcg tcgccaagcg gcagatccca cgcgagccgg cggacctcgc
     2701 ggagcgcatc gcgcggctcg gcgagctgcg cgtgtacacg ccgacctcgc accgggcgat
     2761 gctcgagctg ccgccgtacc tgcaacgccg gctcgagcag ccggggccgg tgatcacggg
     2821 cgagggcgac ctgctgtcct atacgacagg ttcgtaaccg gcgatgtgga gccgcaccaa
     2881 cctgctgatc gtggccgcgg ccgagcggct cggggcgcgg gcggaggcgc tggggcagcg
     2941 gcacacggat tttttgctgc gcctggtcca cggcgagcgc cgcgtgatga tcagcaagac
     3001 gcgctcgccg ttcctcacgc aggtggcgca gaccctggcc aacaacaagc acctgagccg
     3061 cgcgctgctg cgcggtcatg gcgtgccgtg tgtgcccgac ctgctgctcg acgagaccga
     3121 cgacgacgcg gccgcggcgg gcatgctggc gacgcacggg cgggtggtgg tcaagcccaa
     3181 ctgggggaac cgcggcgtcg gggtgacgac ggacgtgcgc acgatggcgg tgctgcgccg
     3241 ggcgcgggcg cgggcgcggg cgatcgacct cgacgaggag gtggtgatcg agccgttcgt
     3301 cgcgggcacg aacctgcgcg tcgcggtggt cggcggtcgc tgcgtcgccg cgtgcgaggt
     3361 gc
//
LOCUS       DASWNJ010000042         3020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_495149, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000042 DASWNJ010000000
VERSION     DASWNJ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495149"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VGB85_00810"
     CDS             complement(<1..727)
                     /locus_tag="VGB85_00810"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEY0132575.1"
                     /translation="MQILDSRRLTGPNLHTGGPAAILQVAFTPDELAEAAIARWRAAV
                     TAALRALGWPLDLHARVHEGHGQRGADLVFTAPIDALYLATEINEWAVAQALTGDEPD
                     PTDMLAGWRDALALEQRPGAIPLRAAAAARDLPVLSDDDSLTIGHGRRALTWPMGQLA
                     DPSAVPWDSLGRVPIAVITGTNGKTTTARLVAHLFRHTGANVGLTTTDGVYLGNRLVI
                     EGDMTGPFSANVILSNPTVDVAVL"
     gene            951..>3020
                     /locus_tag="VGB85_00815"
     CDS             951..>3020
                     /locus_tag="VGB85_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132576.1"
                     /translation="MHHSYDPTQKSSLLLVPVLALTLAACGDDGTATEGASSSGGTTA
                     EGTTTDNPTTNTPTSNTDTTETSPTEPTTDEPTTVAPTTDEVTSTTTGDTTTDPMTST
                     TTGDTTTGVDTTTGVDTTTGVDTTTGVDTTTGVDTTTGVDTTTGGNAEFTGVWATSNP
                     ALLAMDALVKLTPALDAKNATLTVAGDAVSIQSAGVTSGGDTYVTYDAPGMTGGIAVY
                     KDLTDGNPMNKPLGLGTRVIRGPATGLIAPKGIELIGPSGLFIVADTGAADIKVFKLS
                     DSGDVAPQFTITDLGTSAAVWDIHYAANQDVLYAAGTNGEVQVYEDFKLGMGQTGPDR
                     TIVPTVLNTKVSVNLHGITIDSNNLYLSDVGDPMNNTDGQIFVIANASGADGNEDVAQ
                     RIQGGMLGNPVDLELRAGLPRQLYVAEKANDAVLVYAENLMNVLELSKTFVVTKPESV
                     AIVPNTRIMVASNPAGIDMDAAQLLASPLIGDLSVNATFNGLGSVSSIESLVLGASGD
                     GFVSFDGPLPTGGGVFVIPALTGVTMDGVASTLGGRIWGAKTGILTPKGLALNEVGDR
                     LFVADTGAADIKVFAANVLTDVAPVFELNDLGGGPVWDMHYDDASDMLYAAGVDGTVR
                     VFENVLANPGAAPVRVITPTNEQDAKISVNLHGIHYDAASNSLLLSDVGSAMNTTDGQ
                     IFVIAEAS"
BASE COUNT          520 a          985 c         1120 g          395 t
ORIGIN      
        1 ccagcaccgc gacgtcgacg gtcggattcg acaggatgac gttggcggag aaggggcccg
       61 tcatgtcacc ctcgatcacc aggcggttgc cgaggtagac gccgtcggtg gtggtcaggc
      121 cgacgttcgc gccggtgtga cggaagaggt gcgcgacgag ccgcgcggtg gtggtcttgc
      181 cgttggttcc ggtgatcacc gcgatcggga cgcggccgag gctgtcccat gggacagccg
      241 acggatcggc gagctgtccc atcggccagg tcagggcgcg gcggccgtgg ccgatcgtca
      301 ggctgtcgtc gtcgctgagg accgggaggt cgcgggcggc cgccgctgcg cgcaagggga
      361 tggcgccggg gcgctgctcg agcgcgaggg cgtcgcgcca gccggcgagc atgtcggtcg
      421 ggtcgggctc atcgccggtg agggcctgcg cgacggccca ctcgttgatc tcggtggcga
      481 ggtagagggc gtcgatcggg gcggtgaaca cgaggtcggc gccgcgctgg ccgtgaccct
      541 cgtggacgcg cgcgtggagg tcgagcggcc agccgagcgc gcgcagggcc gcggtgacgg
      601 cggcgcgcca gcgggcgatc gcggcctcgg caagctcgtc gggggtgaag gcgacctgaa
      661 ggatcgcggc cgggccaccc gtgtgcaggt tgggtccggt caaacgacgc gagtcgagga
      721 tctgcatgcg cgccggggag cctgtgcccg gcataaccgg cccggcagcc aggggtcaag
      781 ccaggtgacg tcgatgtcgt acgtcgaagc gcgcgtgcgc ggtcgcggcg attgtgtgcc
      841 ccgggacatc ctcatactct caaatgcgaa cccccggcga gccgccggag gaggcccgat
      901 gggcgatcca gccccacgga cccatacgta acgtcgatta ggacaatttc atgcatcatt
      961 catacgaccc gacgcaaaag tcatcgttgc tcctcgtgcc cgtgctggcc ctgaccctgg
     1021 ccgcctgtgg cgacgacggc accgccaccg agggcgcctc cagctccggg ggcacgaccg
     1081 ccgagggcac gaccaccgat aacccgacga ccaacacgcc gacctccaac acggacacca
     1141 cggagacgtc gccgaccgag ccgacgaccg acgagccgac gacggtggcg ccgacgacgg
     1201 acgaggtgac ctcgacgacg accggcgata cgacgacgga cccgatgacc tcgacgacga
     1261 ccggggacac gacgaccggg gtcgacacga cgaccggggt cgacacgacg acgggcgtgg
     1321 acacgacgac gggcgtggac acgacgaccg gcgtggacac gacgaccggt gtcgacacga
     1381 cgaccggcgg caacgcggag ttcacgggcg tgtgggcgac gagcaacccg gcgttgctgg
     1441 cgatggacgc gctggtgaag ctgacgccgg cgctcgacgc caagaacgcg acgctgacgg
     1501 tggcgggcga cgcggtgagc attcagagcg cgggcgtcac gagcggtggc gacacctacg
     1561 tgacctacga cgcgccgggc atgaccggcg ggatcgcggt ctacaaggat ctgacggacg
     1621 gcaacccgat gaacaagccg ctgggcctcg gcacgcgggt gatccgcggg ccggcgaccg
     1681 ggctgatcgc gccgaagggg atcgagctga tcggcccctc gggtctgttc atcgtcgccg
     1741 acacgggcgc ggcggacatc aaggtgttca agctcagcga cagcggcgat gtcgcgccgc
     1801 agttcacgat caccgacctc gggacctcgg cggcggtgtg ggacatccac tacgcggcca
     1861 accaggacgt gctgtacgcg gcgggcacca acggcgaggt ccaggtctac gaggacttca
     1921 agctggggat gggccagacg ggtccggacc gcacgatcgt gccgacggtg ctcaacacca
     1981 aggtgtcggt caacctgcac ggcatcacga tcgactcgaa caacctgtac ctgagcgatg
     2041 tcggcgatcc gatgaacaac acggacggcc agatcttcgt gatcgccaac gcgagcgggg
     2101 cggacggcaa cgaggacgtc gcgcagcgga tccagggcgg gatgctcggc aacccggtcg
     2161 acctcgagct gcgcgccggc ctgccgcgcc agctgtacgt cgccgagaag gcgaacgacg
     2221 cggtgctggt gtacgccgag aacctgatga acgtgctcga gctcagcaag acctttgtgg
     2281 tcaccaagcc cgagtcggtg gcgatcgtgc cgaacacgcg gatcatggtc gcgtcgaacc
     2341 ccgcggggat cgacatggac gcggcccagc tgctcgcgtc gccgctgatc ggtgacctgt
     2401 cggtcaacgc gaccttcaac ggcctcggca gcgtcagctc gatcgagagc ctggtgctcg
     2461 gcgcctccgg cgacggcttc gtcagcttcg acgggccgct gccgacgggc ggcggcgtgt
     2521 tcgtgatccc ggcgctgacg ggggtgacga tggacggggt cgccagcacg ctcggcggcc
     2581 gcatctgggg cgcgaagacg gggatcttga cgccgaaggg gctcgcgctc aacgaggtcg
     2641 gggaccgcct gttcgtcgcc gatacgggcg ccgcggacat caaggtgttc gccgcgaacg
     2701 tgctgacgga tgtcgcgccg gtgttcgagc tcaacgacct cggcggcggt ccggtgtggg
     2761 acatgcacta cgacgacgcg agcgacatgc tgtacgcggc gggcgtcgac ggcacggtgc
     2821 gcgtgttcga gaacgtgctc gccaaccccg gcgcggcgcc ggtccgcgtc atcacgccga
     2881 cgaacgagca ggacgcgaag atcagcgtca acctgcacgg catccactac gacgcggcga
     2941 gcaactcgct gctgctgtcc gacgtcggca gcgcgatgaa caccaccgac ggccagatct
     3001 tcgtgatcgc cgaggccagc
//
LOCUS       DASWNJ010000043         8009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_142217, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000043 DASWNJ010000000
VERSION     DASWNJ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 8009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8009
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142217"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VGB85_00820"
     CDS             <1..282
                     /locus_tag="VGB85_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132577.1"
                     /translation="GAASLRAVILDLRQAPPVNGPSTQQSIGRLLAACEQAGATPALL
                     HRGEGLPALQLGRLVREYAPRLGQAISDLAVAEALAQRAAPVTPSIRRK"
     gene            444..2696
                     /locus_tag="VGB85_00825"
     CDS             444..2696
                     /locus_tag="VGB85_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132578.1"
                     /translation="MRVSLGVCVFVPVLLVGCPPEGEFADESGGTSTSTGTGAVTGSG
                     VTEVMATQGDTGSTTGTGGDTTGTSQPGGTETTSTGSTSDASDTGDTSSGTSGSTGVS
                     STGSTSVDTTSGGVSASTGESSTTGTSVGTGDTSGESSTSTGDTDTGGDELCCEAGCS
                     TFLLKSHVKAVAVQRSATRIASRDADGNLLLWDATSLEQLLVIEDVDAMQMVGDTLVY
                     ASAGQLRILDADDGAQVGEAPAATTFGVAQDESYLWVAGPQGLEVLEPDGAPRWKIAG
                     PLGDAKVLATADAVHVFDADLGPQAVTHYAANDGSPDKVAFLGSFGGWFADTPRYWTT
                     QGNSYRVYDIDDSLIGFKEGAPKYGWGSRMVGFQGVWDIFDPAVMVPISGQAMMSGPA
                     VMTVLWDGQVQNVKLIRLDADPVTATSVTPACCVQEGKAWSFAFADGAWAVGGSDKGL
                     AADDLGRLVTVGEVVGLAGSTAGRVAVGTTDDVVRIYDIADDCEITEHPKFARKGEGL
                     WLSNDGSLLLSSERWKEGMQQNGYVGTRFYSLPDAALLAGAQLQCMACLVLDHEVADD
                     ASAWTRLHTQVPIFYFSGGPFPLGPAYATGPGNVLPKISPDGKHLLTSDGNANQSPEW
                     TGEFSYVRDPVSLVGVFEGIPHGFIDDENFLVGRYTGQASTFVKMDIVGLDGEVVQDT
                     TLPELRKIYRISTGEIVGVAHPSGQAAIYDPWTSAVLWTAPPGTKVVVAGTNHVVVSD
                     LASVELVRWR"
     gene            2764..3972
                     /locus_tag="VGB85_00830"
     CDS             2764..3972
                     /locus_tag="VGB85_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132579.1"
                     /translation="MRRGVCLWIVVAGCGDSLGADGTGSTGTVEATSLSGIATGAPTT
                     GDPPRLDLGGSGCGLGNGDLGLSFIWIANSTQGTISKIDTVALKERGRYVARPDAAGN
                     PSRTSVNLAGDVVVANRNGGLTKVYALAERCAESNGAPGIQTANDANFLKWGEEECVA
                     WHTPMEYFSQRPVAWTQGSFDKATCEFSGQKVWTAGMNIPGTVDFLRLDGDTGAVEAK
                     VTVTDAYVDDYGIYGGAVDARGNFWGSQVGPAKLHFIDYETLEHRSWDMMVGGYGITV
                     DSRGYVWTCAGNASRFDPVSETWTIVAAGEYGGCMEDGAGTLYKATPGGVLAFDTETL
                     TIKKTYKLPQHAHGISVDFNGYVWAVSQGSEAYRLDPADGSYETVGGLVGAYTYSDMT
                     GFALSSLQVP"
     gene            complement(3977..5395)
                     /locus_tag="VGB85_00835"
     CDS             complement(3977..5395)
                     /locus_tag="VGB85_00835"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEY0132580.1"
                     /translation="MRPRRPEHPPHAVDIEETAPAEGNPRASASTTATRLVVVYPRTH
                     AGVHLLDEHEAVLGRHAGHAAQVAVAHPTVSRRHATLRWDPDARRHSLADLGSRNGTW
                     LNQQPVSALPRYLDDQAIVRVGSVIAVYEREDGTVVDPPAVSRTAVPGDSLAVQKLRA
                     AIGRAGPDPSPALILGETGAGKESVAAELHRLSGRRGPQMTINCAALSPQLVESQLFG
                     HTRGAFTGATQEHPGMFRAAHGGTLFLDELGELPIDLQPKLLRAVELGEVVAVGSTQT
                     HRVDVRLVAATNRSLLADIEQGKFRRDLYARLALWEIAVPPLRARRSDLLGWIDRLHA
                     AWAAQRDRADLPPLEFQPDAAEALLLQSWPDNLRGLQRFVHACSAIADPVPIHRAELG
                     PWLANDLRLETSGLAIPTVAHTFNNKKNPRPRPSREALLAILQSNGWSIRATAKHYER
                     DRKQISRWIEMYAIDVPNRSEE"
     gene            5641..6531
                     /locus_tag="VGB85_00840"
     CDS             5641..6531
                     /locus_tag="VGB85_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132581.1"
                     /translation="MTLALGCNSPEYLKWVEEHKNDSASESTGSSDTGTGSTTDAEVS
                     AGSGTGGSTSNGTEAQSSAGVETGEGETTGPMATTSGGPGDSSTTGSSAFCGDGVINQ
                     DFEECDVPSPDPEGPCTAVCQRERIIFVLSISVKGNLSGLQGADAYCKSQATMAMKAD
                     PSSPIKDPNNFKALLSSSKETVFERHFRGEGPYRLVNGLTVSDSFDALFTPPLQNPIN
                     VDEFGHTRNIAVWTATTIDGQPYPGIDFCSDWESNKGSSSYGWSDAIDSTWIDAGDEV
                     IDNPTTDCYDNSALYCVEQE"
     gene            6534..7715
                     /locus_tag="VGB85_00845"
     CDS             6534..7715
                     /locus_tag="VGB85_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132582.1"
                     /translation="MPIDHRIFAVCGALALLAGCPAEFGTGDDGGTTSSSSGDAPPGD
                     DDGGPGLPTSSSGPEPTTSGATSTSGTTEEAAQTCGDGVLDPGEGCDEGIDNAIYAAC
                     TDECQLNVCGDSKVHVGVETCDEGSENVDTGYCRSDCQLGICGDGFVFAALESCDAGG
                     ANGPEYGQCDESCTINRCGDGELDVGHEQCDDGALNGSGEPGELGQAGCDVDCGFFGR
                     RIFLSSQVFTGDMGTRAGADLACEIMATQAGFKAAYRYRALLADSKGSPNSFVVPDPD
                     GRPFILPGGLIVAASYAELIQLGPGEGVTTTEQGEMLFEEKVWTNVNPAGDAYLDDPA
                     STCAEWSSADKLKSARAGINAVAPGDAAALAEWRAQKHWLSAVNLICSKQYRIYCIEA
                     S"
BASE COUNT         1261 a         2452 c         3098 g         1198 t
ORIGIN      
        1 ggcgccgcca gcctgcgcgc ggtgatcctc gatctgcgtc aggcgccgcc cgtcaatgga
       61 ccctcgacgc agcaaagcat cggccggctg ctcgccgcct gcgagcaggc cggtgcgacg
      121 cccgcgctgc tgcaccgcgg cgaggggctc ccggcgctgc agctcggccg cctggtccgt
      181 gaatatgccc cgcggctcgg ccaggccatc agcgacctcg ccgtcgccga ggcgctggcg
      241 cagcgggccg cgccggtgac cccgagcatt cgccgaaagt gagagagcgg cttcgcggac
      301 accgacggtt ttgtgacgag gtggagaatg cccgtcgctc acgtaatgtg cattgatacg
      361 cgatatcgtg gctccagcgg cggcatcgtc gggtccgatc ggcgcggctt gcccggcgcg
      421 gctcgccggg gtagtcagcg atgatgcgcg tctctctcgg tgtatgtgta tttgtcccgg
      481 tcctgctcgt cggctgcccg ccggaagggg agttcgccga cgagagcggc ggaacgagca
      541 cgagcaccgg cacgggcgcg gtgacgggct ccggcgtgac ggaggtgatg gcgacgcagg
      601 gtgacacggg cagcacgacg ggcacgggcg gggacacgac cggcacctcg cagcccggag
      661 gtacggagac gacgagcacg ggcagcacga gcgacgcgag cgacacgggc gatacgagca
      721 gtggcacctc tggcagcacg ggcgtgagca gcacgggcag cacatcggtg gacacgacga
      781 gcggcggcgt ctctgccagt acgggcgaga gcagcacgac cggcacatcc gtgggcacgg
      841 gcgacacgag cggcgaaagc tctaccagca cgggcgacac ggacacgggc ggcgacgagc
      901 tgtgctgtga ggccggctgc agcacctttt tattaaaatc gcatgtgaag gcggtcgcgg
      961 tgcagcgcag cgcgacgagg atcgcgtcgc gcgacgcgga cgggaacctg ctgctgtggg
     1021 acgcgacgag cctcgagcaa ttgttggtaa tcgaggatgt cgacgcgatg cagatggtcg
     1081 gcgatacgct ggtgtacgcg tccgcggggc agctgcgcat cctcgacgcg gacgacggcg
     1141 cgcaggtcgg tgaggcgccg gcggcgacga ccttcggtgt ggcccaggac gagagctatc
     1201 tgtgggtcgc ggggccgcag gggctcgagg tgctcgagcc ggacggcgcg ccgcgctgga
     1261 agatcgcagg cccgctcggc gatgcgaagg tgctggcgac ggcggacgcg gtgcacgtgt
     1321 tcgacgccga cctcgggccg caggcggtga cgcactacgc ggcgaacgat ggcagcccgg
     1381 acaaggtggc gttcctcggc agcttcggcg ggtggttcgc ggacacgccg cgctactgga
     1441 cgacgcaggg caactcgtat cgcgtctacg acattgacga cagcctgatc gggttcaagg
     1501 agggcgcgcc gaagtacggc tggggttcgc gcatggtcgg ctttcagggg gtatgggaca
     1561 tcttcgatcc ggcggtgatg gtgccgatct ccgggcaggc gatgatgtcg gggccggcgg
     1621 tgatgacggt gctgtgggac gggcaggttc agaacgtgaa gctgatccgc ctcgatgccg
     1681 acccggtcac ggcgacgtcg gtgacgccag cgtgctgcgt gcaggagggc aaggcctgga
     1741 gcttcgcgtt cgcggacggg gcgtgggcgg tcggcggcag cgacaagggg ctggcggcgg
     1801 acgacctcgg gcgcctggtg acggtcggtg aggtggtcgg gctggcaggc tcgacggcgg
     1861 gccgcgtcgc ggtcgggacc accgatgatg tcgtgcggat atacgacatc gcggacgact
     1921 gcgagatcac cgagcacccg aaattcgcgc gcaagggcga ggggctgtgg ctgtccaacg
     1981 acggctcgct gctgctgtcg agcgagcgct ggaaggaggg catgcagcag aacggctacg
     2041 tcggcacgcg gttttattcg ctgccggacg cggcgctgct ggccggcgcg cagctgcagt
     2101 gcatggcctg cctcgtgctc gatcacgagg tcgcggacga cgcgagcgcg tggacgcggc
     2161 tgcacacgca ggtcccgatc ttctacttca gcggcgggcc gttcccgctc ggtccggcgt
     2221 acgcgaccgg cccgggcaac gtgctgccga agatctcgcc cgacggcaag cacctgctga
     2281 ccagcgacgg caacgccaac cagtcgccgg agtggaccgg cgagtttagc tatgtgcgcg
     2341 acccggtgtc gctcgtcggg gtgttcgagg gcatcccgca cggctttatc gacgacgaga
     2401 attttttggt cgggcggtac accggccagg cgagcacttt tgtgaagatg gacatcgtcg
     2461 gcctcgacgg tgaggtcgtg caggacacga cgctgccgga gctgcgcaag atctaccgca
     2521 tcagcacggg cgagatcgtc ggggtcgcgc atccgtccgg gcaggccgcg atctacgatc
     2581 cgtggacgag cgcggtgctg tggacggcgc cgccgggtac gaaggtggtc gtcgccggga
     2641 cgaaccacgt ggtcgtcagc gacctcgcca gcgtcgagct ggtgcgctgg cgctgagcgg
     2701 tcccggacgc cagcggggag gccgccgcga cggcgcggcg agcctcggct aaggtgtttc
     2761 gtgatgcggc gcggcgtgtg tttgtggatc gtggtcgcgg gctgcgggga cagcctcggg
     2821 gcggacggga ccggctcgac ggggacggtg gaggcgacga gcctgtcggg gatcgcgacc
     2881 ggcgcaccga cgacgggcga tccgccgcgg ctcgacctcg gaggatcggg ctgcggtctg
     2941 ggcaacgggg acctcgggct gtcgttcata tggatcgcca acagcacgca gggcacgatc
     3001 agcaagatcg atacggtggc gttgaaggag cgcgggcggt acgtcgcgcg gccggacgct
     3061 gcgggtaatc cttcgcgcac gtcggtgaac ctcgccggtg acgtcgtggt cgccaatcgc
     3121 aacggcggcc tgacgaaggt gtacgcgctc gcggagcggt gcgccgagag caacggagcg
     3181 ccggggatcc agacggcgaa cgacgcgaac ttcttaaaat ggggggagga ggagtgcgtc
     3241 gcgtggcata ctccgatgga gtacttcagc cagcggccag tcgcgtggac gcagggaagc
     3301 tttgataagg cgacgtgtga attttccggg cagaaggtgt ggacggcggg gatgaacata
     3361 ccgggcactg tcgactttct gcggctcgac ggcgacacag gggcggtcga ggcgaaggtg
     3421 acggtcaccg atgcgtatgt cgacgactat gggatctacg gcggagccgt cgatgcgcgc
     3481 gggaacttct ggggctcgca ggtcgggccg gcgaagctgc actttatcga ttacgagacg
     3541 ctcgagcacc gcagctggga catgatggtc ggtgggtacg ggatcacggt cgactcgcgc
     3601 gggtatgtgt ggacctgcgc gggcaatgcc tcgcgcttcg acccggtgag cgagacctgg
     3661 acgatcgtcg cggccggcga gtacggcggc tgcatggaag acggcgccgg caccttatat
     3721 aaggcgacgc cgggcggggt gctggccttc gataccgaga cgctgaccat taaaaagacg
     3781 tacaagctgc cacagcacgc gcacgggatc agcgtcgatt ttaatggcta cgtgtgggcg
     3841 gtgtcgcagg gcagcgaggc gtatcgcctc gacccggcgg acggcagtta cgagaccgtc
     3901 ggcgggctgg tcggcgctta cacgtacagc gatatgaccg gctttgcgct cagctcgctg
     3961 caggtgccct gagagatcat tcttcgctgc ggttcgggac gtcgatcgcg tacatctcga
     4021 tccagcgcga gatctgcttg cggtcgcgct cgtagtgctt ggcggtcgcg cggatgctcc
     4081 agccgttgct ctgcaaaatt gccagcaggg cctcgcggga ggggcgtggg cgggggtttt
     4141 tcttattatt aaaagtgtga gcgacggtgg ggatggcgag gccgctcgtc tcgaggcgga
     4201 ggtcgttggc gagccagggg ccgagctcgg cgcggtggat cgggacgggg tcggcgatcg
     4261 cgctgcaggc gtggacgaag cgttgcaggc cgcgcaggtt gtcgggccac gattgcagca
     4321 gcagggcctc ggcggcgtcg ggctggaact cgagcggggg caggtcggcg cggtcgcgct
     4381 gggcggccca ggcggcgtgc aggcgatcga tccacccgag caggtcggac cggcgggcgc
     4441 gcagcggcgg gacggcgatc tcccacagcg cgaggcgagc gtagaggtcg cggcggaact
     4501 tgccctgctc gatgtccgcg agcaacgagc ggttggtggc ggcgacgagg cggacgtcga
     4561 cgcggtgggt ctgcgtgctg ccgacggcga cgacctcgcc gagctcgacg gcgcgcagca
     4621 gcttcggctg caggtcgatc gggagctcgc cgagctcgtc gaggaagagg gtgccgccgt
     4681 gcgcggcgcg gaacatgcca gggtgctcct gcgtggcccc ggtgaaggcg ccgcgggtgt
     4741 ggccgaacag ctggctctcg acgagctgcg ggctgagggc ggcgcagttg atcgtcatct
     4801 gcgggccgcg acggccggac aggcggtgca gctcggcggc gacgctctcc ttgccggccc
     4861 cggtctcgcc gaggatcagc gcgggcgagg ggtcgggccc ggcgcggccg atcgcggcgc
     4921 gcagcttctg aaccgcgagc gagtcgccgg ggaccgcggt gcgcgagacg gcgggcgggt
     4981 cgacgacggt gccgtcctcg cgctcgtata cggcgatcac gctgccgacg cggacgatcg
     5041 cctggtcgtc gaggtagcgc ggcagggcgc tgaccggttg ttggttgagc caggtgccgt
     5101 tgcggctgcc gaggtcggcg aggctgtggc ggcgcgcgtc cgggtcccag cgcagggtcg
     5161 cgtgccggcg ggagacggtc gggtgcgcga cggcgacctg cgccgcgtgg ccggcgtggc
     5221 ggccgagcac ggcctcgtgc tcgtcgagca ggtgcacacc cgcgtgggtg cggggataca
     5281 cgacgacgag gcgcgtggcg gtcgtgctgg cgctggcgcg cgggttgccc tcggcggggg
     5341 cggtctcctc gatgtcgacc gcgtgcggcg ggtgttcggg gcgacggggc cgcatggcgc
     5401 ggcgatggtg tcgcacgggc cgcgtggggg caagaccgga ggcgggtggg atgtgcgggg
     5461 gccgtcgccg cggtgtcgtg agtacgacgt tcggcgggcc gaggtgatgt cgggcctggc
     5521 cgtatctctg gcggtgaatg agctgcttgg atacaaatac cagtgcgccg ggcgtgattg
     5581 acgtgcgcga ttttgtgtgg tcagttcgac ctcgagatgc ggcccctgct gacaccactg
     5641 atgaccctgg ctttggggtg taattcgccc gagtatctga agtgggtcga ggagcacaag
     5701 aacgactccg cgagcgaatc caccgggagc tctgacaccg gcacgggcag cacgaccgac
     5761 gcggaggtca gcgccgggag cgggaccggg gggagcacga gcaatggcac cgaagcgcaa
     5821 agcagcgctg gcgtggagac cggcgagggc gagaccaccg ggccgatggc gaccacgagc
     5881 ggaggaccgg gcgacagctc gacgaccggc tcctcggcgt tttgcgggga tggcgtcatc
     5941 aaccaggact tcgaggagtg cgacgtcccg agccccgacc ccgagggccc gtgcacagct
     6001 gtctgtcagc gcgagagaat tatttttgtc ctcagcatct cggtcaaggg caacctgagc
     6061 ggcctgcagg gcgcggacgc ctactgtaaa tcgcaggcga ccatggcgat gaaggcggac
     6121 ccgtcctcgc cgatcaagga ccccaataat ttcaaggcgc tgctgtcttc ctcgaaggag
     6181 acggtgttcg agcgccactt tcgcggcgag ggcccgtacc ggctcgtcaa cggcctcacc
     6241 gtcagcgaca gcttcgacgc cctgttcacc cctccgctgc agaacccgat caacgtcgac
     6301 gagttcgggc acacgcgcaa catcgcggtg tggaccgcga cgaccatcga cggccagccc
     6361 tatccgggca ttgacttctg ctccgactgg gagagcaaca agggctcgtc cagctacggg
     6421 tggagcgatg ccatcgactc gacctggatc gacgccggtg atgaggtcat cgacaacccc
     6481 accaccgact gttacgacaa cagcgcgctg tactgcgtgg agcaggagta gagatgccga
     6541 tcgaccaccg catttttgcc gtgtgtggcg ccctcgccct gctcgccggt tgtcccgcgg
     6601 agttcggcac gggcgacgac ggcggaacca cctcgtcctc gagcggcgac gctccgcccg
     6661 gggatgacga cggtggcccg ggtctcccga cctcgtcgag cggccccgag ccgaccacga
     6721 gcggcgccac gagtacctcc gggacgaccg aagaggccgc ccagacctgc ggcgacggcg
     6781 tgctcgatcc tggcgagggc tgcgacgagg gcatcgacaa cgcgatctac gccgcctgca
     6841 cggacgagtg ccagctcaac gtctgcggcg acagcaaggt ccacgtcggg gtcgagacct
     6901 gcgacgaggg cagcgagaac gtcgacaccg ggtactgccg ctcggactgc cagctcggga
     6961 tctgcggcga cggattcgtg ttcgcggcgc tcgagtcctg cgatgcaggc ggggcgaatg
     7021 gcccggaata cggccagtgc gacgagagct gcaccatcaa ccgctgtggc gacggcgagc
     7081 tcgacgtcgg ccacgagcag tgtgacgacg gggccctcaa tggttcgggc gagcccggcg
     7141 agctcggtca ggccggctgc gacgtcgact gcggattctt cggacgccgg atcttcctgt
     7201 catcccaggt attcacgggc gacatgggca cccgcgcggg cgccgatctg gcctgcgaga
     7261 tcatggcgac gcaggcgggc ttcaaggctg cctacaggta ccgggccctg ctcgccgaca
     7321 gcaagggctc ccccaacagc ttcgtcgtgc ccgatccgga cggccggccc ttcatcctcc
     7381 cgggtggcct catcgtcgcc gccagctacg ccgagctgat ccaactcggg cccggcgagg
     7441 gcgtcaccac caccgagcag ggcgagatgc tgttcgaaga gaaggtgtgg accaacgtca
     7501 acccggccgg tgacgcgtac ctcgacgacc ccgcgtcgac ctgcgccgag tggagctccg
     7561 cggacaagct caagtcagcc cgcgccggca tcaacgccgt cgcgcccggc gatgcggccg
     7621 cgctcgccga gtggcgggcc caaaagcact ggctcagcgc cgtgaacctg atttgcagca
     7681 agcagtaccg catctactgt atcgaggcca gttgacgcgc gtgggaggcg ctcgatgggc
     7741 gccgggcgaa ttgagacctg gcgtgcacgg tcgctagaga cgttcgtcga tgggccagca
     7801 tccttgtggc tgctggccgg gacccgggtc gtgatagggt cgatgcggtg ctcagcggcg
     7861 gggtgcagac ggtgctcggg cgcctgcgac ggggcgcggc ggacgggcag gcgcgtcgcg
     7921 gcttcacggt ggcgcgtacg gaggcgctgc tgcggctgcg cgacgtgccg agagtgcagt
     7981 gggcgtggac gctggggttg ttgcgggcg
//
LOCUS       DASWNJ010000044         2800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_566157, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000044 DASWNJ010000000
VERSION     DASWNJ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566157"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..1250)
                     /locus_tag="VGB85_00850"
     CDS             complement(246..1250)
                     /locus_tag="VGB85_00850"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0132583.1"
                     /translation="MDVNAGTSTTPAATPGGTGSASTSASTSAVLALIGTQLLFGSHY
                     SVAREITARLDPVAWTVLRALLGLATLVLVMLVMRRRWPRGRAMWAQLTLLGLLGVSL
                     NQLLFNAGMARSTAIHGVLLMATVPAQTLALGVLLGQERFSPRKLTSVLFGVVGVAVL
                     MRVDTVATADPGAWWQTRDGLPAASFGETLLAGDLMILANSACYALFLALGKSTTRTL
                     DPLALCVGVYTTGAAMVCLIGGPALWATDLTALPAFVWWMIAFVVLGPTVGAYLLNMY
                     ALARLPTSLVGLFINLQFVIAVGVAMLWHGEAFDARIVIAGLLVLGGVSLRFRPESLG
                     "
     gene            1369..1914
                     /locus_tag="VGB85_00855"
     CDS             1369..1914
                     /locus_tag="VGB85_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEY0132584.1"
                     /translation="MHPWHDIYVDEQVIAAGFPAVIEVPQGSKNKYEVDKETGFLRLD
                     RVLFSAVHYPANYGFIPRTYCDDGDALDVLVLQQSPVHPLCIVTARPIGVMRMRDDKG
                     IDDKIVAVSTHDPWFSDYTDQSQLPGHIMREVQRFFEDYKILEKKAVVIDGMLGAADA
                     LQIIRDGLDLYKRLRRGELHK"
     gene            1939..2652
                     /locus_tag="VGB85_00860"
     CDS             1939..2652
                     /locus_tag="VGB85_00860"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY0132585.1"
                     /translation="MVADPHEDAALLQAWQAGDRDAGNRLVDRHFDAITRFFRNKVCA
                     EDDVAELVGQTFLGCTSGKDRFRGDASVRQYLYAIAQNVLRKYIRARYKRQSEHLDFA
                     AVCVQDLAPASLSSIVAHKREAQSLVQALREIPVEDQVVLELMYFEGLSGSELAGVLG
                     LPEGTVRGRLARGKQRLRERVAALLARAPAAARSAPPAAHGAVTPDRPDRPDQLDQLD
                     QLEQLDHWALELRRAQGWA"
BASE COUNT          464 a         1049 c          915 g          372 t
ORIGIN      
        1 gcccgcggcg aaaaggcccc cgtggcccat cgcagccgcc gtcgcgtcga gctcggcgcc
       61 ccgctgacca gccgcgccgg cgagccctcg gcccgccccc gcccctcccg ggtcctggcc
      121 gagctgcacg ccgccctcgc cgacgaggcc gccgcggccg agtaactcgc tgttactcgc
      181 tcttactcgc cgttccatgt ccgccggggc atgtccacga ggacatgccc cttcggtcag
      241 gtatctcagc ccagggactc gggccgaaac ctcagcgaca ccccgccgag caccagcagc
      301 ccggcgatga cgatgcgcgc gtcgaaggcc tcgccgtgcc acagcatcgc cacgccgacc
      361 gcgatcacga actgcaggtt gatgaacagg ccgaccagcg aggtcggcag gcgggcgagc
      421 gcgtacatgt tgagcaaata ggcgccgacg gtcggtccga ggacgacgaa ggcgatcatc
      481 caccacacga acgccggcag ggccgtcagg tcggtcgccc acagcgccgg cccgccgatc
      541 aggcacacca tcgccgcgcc ggtcgtgtag acgccgacgc acagcgccag cgggtcgagg
      601 gtccgcgtcg tcgacttgcc gagcgcgagg aacagcgcgt agcaggccga gttggcgagg
      661 atcatcaggt cgccggccag cagcgtctcg ccgaagctcg ccgccggcag accgtcccgc
      721 gtctgccacc acgcgcccgg gtccgccgtc gccaccgtgt cgacccgcat cagcaccgcc
      781 accccgacga ccccgaacag cacgctcgtc agcttgcgcg gcgagaagcg ctcctggccg
      841 agcagcaccc cgagcgccag cgtctgcgcc ggcaccgtcg ccatcaacaa caccccgtgg
      901 atcgccgtcg agcgggccat gccggcgttg aacaggagct ggttgagcga caccccgagc
      961 agcccgagca gcgtcagctg ggcccacatc gcccgccctc gcggccagcg cctgcgcatc
     1021 accagcatca ccagcaccag cgtggcgagc ccgagcagcg cgcgcagcac cgtccacgcg
     1081 accgggtcga gccgcgccgt gatctcgcgg gcgacgctgt agtgcgagcc gaacagcagc
     1141 tgcgtgccga tcagggccaa aacagccgaa gtggacgctg aagtggaagc cgagcccgtg
     1201 ccgccgggcg tggccgcggg cgtcgtcgag gtgcccgcgt tcacgtccac gtcccgatgc
     1261 ctagcacggg ccgatcgggc gtgcggcgag tgtccggctt gagcggacac acgggcgtgt
     1321 gtccctcgct tgacaggccc gccgcgctgg acgagcgtgg cccccgcaat gcacccctgg
     1381 catgacatct acgtcgacga gcaagtgatc gcggccggct tcccggcggt gatcgaggtc
     1441 ccgcagggca gcaagaacaa gtacgaggtc gacaaggaga ccggcttcct ccgcctcgac
     1501 cgcgtgctgt tcagcgcggt gcactacccc gccaactacg gcttcatccc gcgcacctac
     1561 tgcgacgacg gcgacgccct cgacgtgctc gtgctgcagc agagccccgt gcacccgctg
     1621 tgcatcgtca ccgcgcgccc gatcggcgtg atgcgcatgc gcgacgacaa gggcatcgac
     1681 gacaagatcg tcgccgtcag cacgcacgac ccatggttta gcgactacac cgaccagtcc
     1741 cagctgcccg gccacatcat gcgcgaggtc cagcgcttct tcgaggacta caaaattctc
     1801 gagaagaagg cggtcgtcat cgacggcatg ctcggcgccg ccgatgccct ccagatcatc
     1861 cgggacgggc tcgacctcta caagcgcctg cgtcgcggcg agctgcacaa gtagcccgcc
     1921 ctgacgtatg atggtcgcgt ggtcgccgac ccacacgagg acgccgcgct gctgcaggcc
     1981 tggcaggccg gcgaccgcga cgccggcaac cgcctcgtcg accgtcactt cgatgcgatc
     2041 acccggttct tccgcaataa agtgtgcgcc gaggacgacg tcgccgagct ggtcggccag
     2101 accttcctcg gctgcacgag cggcaaggat cgcttccgcg gcgacgcgag cgtgcgtcag
     2161 tatctgtacg cgatcgccca gaatgtccta cgcaagtaca tccgcgcccg ctacaagcgc
     2221 cagagcgagc acctcgactt cgccgccgtg tgtgtacaag atctcgcccc cgcctcgctg
     2281 agctcgatcg tcgcgcacaa gcgtgaggcc cagagcctgg tccaggcgct gcgggagatc
     2341 ccggtcgagg atcaggtcgt gctcgagttg atgtacttcg agggcctctc gggcagcgag
     2401 ctcgccggcg tgctcggcct gcccgagggc accgtccgcg gccgcctcgc gcggggcaaa
     2461 cagcgcctgc gcgaacgcgt cgccgcgctg ctggctcgtg cccccgcagc ggcccgctcg
     2521 gcgccccctg ccgcccatgg tgcggtcacc cctgatcggc ctgatcggcc tgatcaactc
     2581 gatcaactcg atcaactcga acagctggat cactgggcgc tcgagctgcg acgcgcgcag
     2641 ggctgggcct gagcgaacac acccgcgcgg cgcgatcgag tgcatgtgca cttgcgcgcc
     2701 gctgagcaga cgcgcagcga agacggtgcg ctgcgtcgcc gcggccgccg agtcggatcg
     2761 tctatagtgg cagcttgcag gcgcccgggg gcgcgggatt
//
LOCUS       DASWNJ010000045         1709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_637007, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000045 DASWNJ010000000
VERSION     DASWNJ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637007"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            407..1249
                     /locus_tag="VGB85_00865"
     CDS             407..1249
                     /locus_tag="VGB85_00865"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEY0132586.1"
                     /translation="MRTRHFGYTDTGRRRRHNEDAWLADEKLGLFIVCDGVGGMARGE
                     VASEETIELIWDWVKGQAQVIQAAIAEAEAARASGSLAVGDTVTRADGPRAWMPEEAV
                     GRLGALVRGAVQNACYMVHGMGEVDPSQRGMSTTASVVLVAGELVVVGQVGDSRVYLA
                     RDGVVSQLTEDHTWLNVQVKQGVITPEQARRKASTGKHMITRAVGLREFVEVDILALP
                     VQVGDRLLLCSDGLHAYLDAADTLIDLFRLDLREAGPAAIRHANACGGEDNITALFVE
                     LLAT"
     gene            1251..>1709
                     /locus_tag="VGB85_00870"
     CDS             1251..>1709
                     /locus_tag="VGB85_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132587.1"
                     /translation="MARTYTPADLAIFDLLQAPVWVVDLDRGAQWWANLACLPLWHAT
                     DRAQMLANSANSSPSETSRTRLEVLRRKFQRGETSLDRWTLYPDDGAPFIAECRSSGI
                     VIADEPGVPGRLAMLIEARVLGPDEFDPHERRGVEALRYLGELVALHTDAG"
BASE COUNT          241 a          621 c          610 g          237 t
ORIGIN      
        1 tgcgttgcat ggcgctcccg ataaggtgag gtgaggcgag gtgagccgcc ggaccgtcgg
       61 cccgacgtga acgggcaacg taacgcatat gcaggcgtcc tggccacgcg tcgcgagccg
      121 gacttccttg cactgcgacg ccgatccgca cgcccctgaa gcatcgcgtg ctcaaccgac
      181 gcccggactc gacgggtgcg tccggtgacg cggcccgccg cgctggcgag cgtgatcatg
      241 acgggcgccg cgagcttcgg ggcatgcgtg ccgacatcgc ggcgccgtgt ccctgctcgg
      301 cgcgctccgg gccagcccgg caccaaaatg gcgagaatgc gcgtctgcgc gcctaccggc
      361 ggacctgcta tctcggccga cttgtcggtg ttatcgtggc gcggcgatgc gcacgcgtca
      421 cttcggctac acggacacgg gccggcgccg cagacacaac gaggacgcct ggctggccga
      481 cgagaagctc ggcctgttca tcgtatgcga cggcgtcggc ggcatggcgc gcggcgaggt
      541 cgccagcgag gagaccatcg agctgatctg ggactgggtg aagggccagg cgcaggtgat
      601 ccaggccgcg atcgcagaag ccgaggccgc gcgcgcgagc ggctcgctcg cggtcggaga
      661 caccgtcacg cgcgccgacg gtccgcgcgc gtggatgccc gaggaggccg tcggccggct
      721 cggcgcgctg gtccgcggcg cggtgcagaa cgcctgttac atggtccacg gcatgggtga
      781 ggtcgacccc tcgcagcgcg gcatgagcac gaccgcctcg gtggtgctcg tcgcgggcga
      841 gctggtggtc gtcgggcagg tcggcgactc gcgggtctac ctcgcgcgcg acggcgtcgt
      901 ctcgcagctg accgaggatc acacctggct caacgtacag gtcaagcagg gcgtgatcac
      961 gccggagcag gcgcgccgca aggcgagcac cggcaagcac atgatcacgc gcgccgtcgg
     1021 cctgcgggag ttcgtcgagg tcgacatcct cgcgttgccg gtgcaggtcg gcgaccgcct
     1081 gctgctgtgc tcggacggcc tgcacgccta cctcgacgcc gccgacacgc tgatcgacct
     1141 gttccgcctc gacctgcgcg aggccgggcc ggccgcgatc cgccacgcca acgcgtgcgg
     1201 cggcgaggac aacatcaccg cgctgttcgt cgagctcctg gcgacctgag atggctcgca
     1261 cgtacacccc cgcggacctg gcgatcttcg acctgttgca ggcgccggtc tgggtggtcg
     1321 acctcgaccg cggcgcgcag tggtgggcca acctcgcctg cctgccgctg tggcacgcga
     1381 ccgaccgcgc gcagatgctc gccaacagcg ccaacagctc gccctccgag acctcgcgga
     1441 cccgcctgga ggtgctgcgc cgcaagttcc agcgcggcga gacctcgctc gaccgctgga
     1501 ccctgtaccc cgacgacggc gcgcccttca tcgccgagtg ccggtcctcg ggcatcgtca
     1561 tcgccgacga gcccggcgtc cccggccgtc tggcgatgct gatcgaggcc cgcgtgctcg
     1621 ggcccgacga gttcgacccc cacgagcgcc gcggcgtcga ggccctgcgc tacctcggcg
     1681 agctcgtcgc cctgcacacc gacgccggc
//
LOCUS       DASWNJ010000046         5461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_601358, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000046 DASWNJ010000000
VERSION     DASWNJ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601358"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="VGB85_00875"
     CDS             <1..1041
                     /locus_tag="VGB85_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132588.1"
                     /translation="GSTLIGSVLRTFDELVLAVTPENTLVQVDLADAAVTILQPDVIG
                     FDASRDGRYVLWQDTTVTKADPELPDGKIFLRDRSTGSDTFLAETSLGFSGFPLRHAQ
                     DGLVELNLGYINRDPTRLYFLPELDSIDVPGDLFINGKLDDGRWLVSPGWSSYYDLVD
                     LRNGGEATRLFPRKADLTSVGSEAIELLEIPQCCIEGDFRDEGPMWRVPLDGSAPEPM
                     ARRATRLMRRTADGRIITPVSIDEHWLAPLLLVDPATQTEQHLDDRVFAHSVDPAWTG
                     DTDIISYSVSDGERSGVYLARLAPQDRKAPSPRPREIAAYVVDLMPDPDGRPTPRPRA
                     LTATPDLPVGTK"
     gene            1096..4308
                     /locus_tag="VGB85_00880"
     CDS             1096..4308
                     /locus_tag="VGB85_00880"
                     /inference="COORDINATES: protein motif:HMM:NF042871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEY0132589.1"
                     /translation="MLAESRKYQSDISTRHAEQALGALLELLRGFQAADEATRGALLG
                     ALSRDDPQHIYGGLLTVLLRLVFLLHAEDRGLMPCASTGATRHSVTGLYLRLREDAGR
                     DPDTMAQRFGAWSALLALFRLVHTGATDDRLHLPAREGSLFDPDGYPFLEGRVRGQPD
                     ARRPPPRVPDGVLWRILQSLLLLDGERLSYRSLDVEQIGGVYESMIGFKLERTNGPSI
                     AVRPQHVVVDLADLLSQKPDARAKFLKAQAGCDLTGASLAALKSADTPEDLVAALGRK
                     QSPHTPAIVPAGSLVLQPGEERRKSGSHYTPRSLTGPIVRATLRPVLAALGDAPTPQQ
                     ILDLKLCDPAMGSGAFLVEAARQLGEQLRRAWELHRAAPPIPPDDDLDLHARRLVAQR
                     CLHGVDRDPFAVDLARLSLWLVTAAKDHPFTFVDHALKHGDSLVGLSNSQIADFRWDH
                     ETRDDGVLFAQIRQQVGEASALRREAHNYGDHQDAAKRSTLKLAEAAVHQARLIGDAA
                     ILAYFSRDSDKHRNTRREEVYRDTIIPAQKGDPRALDRLTQLAAELRGGDRPVTPFHW
                     ELEFPEVFDREDPGFDCFVGNPPFLGGKRISTVLGNVTRDWLAQAHVNASSNADLAAH
                     FFRRAYHLLRATGTFGLIATNTITQGDTLRAGLSYICHDGGVIYEADRRIRWPGAAAV
                     MVCVVHVARARAGVSPPHRLDQRPVDRISAFLFHAGGDEMPVPLRSNTGISFIGCDIK
                     GAGFTFDDTVPDATPVARMRELLALDASNAEVVRPYIGGEEINSDPRQAPHRYVIHFG
                     EMPLEAASRWPALLQIVEEKVRPRRLAMARDLARWPWWQFWRVRARLAEKTRHLSSVL
                     VNCQVTAHLSFAFLPTHYVFAHTVNVFGVDRHGFFAILQSRSHEIWARFFGSSMKDDL
                     RYTPSDCFETFPLPPDWQHDAALAQAGKRYYELRADLMVRNNEGLTAIYNRFHNPDER
                     SPDILRLRDLHAEMDRTVLTAYGWPDLAARATCEFRLDYEDDNDDPPRARTRKKPWRH
                     RWPQAFHDEVLARLLDLNHQRASQERPARPPAALLPPDDD"
     gene            complement(4374..>5461)
                     /locus_tag="VGB85_00885"
     CDS             complement(4374..>5461)
                     /locus_tag="VGB85_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132590.1"
                     /translation="LEAIEMQVLTLHEVRALAADVAREGANPRRVLDEYVRLLADRFK
                     GQPPLPLYQAVGGEDEQRFVTILREVSARLREPASIVSDDELFSRSYLAIDLRFAPGQ
                     FLSTQAVTSYYEDFRRAMRSLARYGTGRTGRVEKPIEIATDFALESMQITPLNGVPAR
                     ARLELGAPYAQLDFRGGKVHEAIDQIMDLASRADAQGFDAVLDFGGDLDAETRTRALV
                     QTMRVLPRGGIEEVTLGGTYTARAPVRLRKLHAPKLMAAVAKDMEPSNYDQITLIRAI
                     DLDRGSLIHGRTPRDRIACHASSDLLEGVTTVGVLARIVGKRYEPPSGSPFVIVEQIE
                     LVSERADVIDYESRQIGGATRRNGKAR"
BASE COUNT          900 a         2111 c         1599 g          851 t
ORIGIN      
        1 ggctccaccc tcatcggcag tgttttgcgc accttcgacg agctcgtgct cgccgtcacg
       61 cccgagaaca cgctggtcca ggtcgacctc gccgacgccg cggtgacgat cctacagccc
      121 gatgtgatcg gcttcgacgc ctcgcgcgac gggcgatatg tgctgtggca ggacacgacc
      181 gtcacaaaag cggatcccga gctgccggat ggcaagatct tcctgcgcga ccggtcgacc
      241 ggcagcgata catttttggc agagacctcg ctgggcttca gtggcttccc gctgcgacac
      301 gcccaggacg gtctggtcga gctcaacctc ggctatatca atcgtgatcc cacgcggctg
      361 tatttcctcc ccgagctcga ctcgatcgac gtccccggcg acctgttcat caacggcaag
      421 ctcgacgacg gacgctggct cgtcagcccg ggatggagca gctattacga cctggtcgac
      481 ctgcgcaacg gcggcgaggc gacccgcctg ttcccgcgca aggccgacct cacgagcgtc
      541 ggcagcgagg cgatcgagct gctcgagatc ccgcaatgtt gcatcgaggg cgacttccgc
      601 gacgagggcc cgatgtggcg cgtaccgctc gacggctcgg cacccgagcc gatggcgagg
      661 cgcgcgacgc gtctcatgcg acgaacggcg gacggccgca tcatcacgcc ggtgagcatc
      721 gacgagcact ggctcgcgcc cctgctgctc gtcgaccccg cgacgcagac ggagcagcac
      781 ctggacgacc gggtgttcgc ccactccgtc gatccggcct ggacgggcga tacggacatc
      841 atcagctact cggtgtccga cggcgagcgc tccggcgtgt atctcgctcg cctggcgccc
      901 caggaccgca aggcgccgag cccccggccc cgcgagatcg cggcatacgt ggtcgacctg
      961 atgccggacc ccgacggccg cccgacaccg cgaccccgcg cgctcacggc gacgccggac
     1021 cttcctgtcg gtacaaagta gagcctcccg tttggctggc cgggtccgcg cgtccccgtg
     1081 ataccttgca ccctcgtgct cgccgagtcg cgcaaatacc agagcgacat ctccacccgc
     1141 cacgccgagc aggccctcgg cgccctgctc gagctcctgc gcggcttcca ggccgccgac
     1201 gaggccaccc gcggcgcgct gctcggcgcc ctctcgcgcg acgaccccca gcatatctac
     1261 ggaggcctgc tcaccgtcct cttgcgcctc gtcttcctgc tccacgccga ggaccgcggc
     1321 ctgatgccct gcgcgtcgac gggggccacc cggcacagcg tcaccggcct gtacctgcgc
     1381 ctgcgtgagg acgccggccg cgaccccgac accatggcgc agcgcttcgg cgcctggtcg
     1441 gccctgctgg cgctgtttcg cctcgtccac accggcgcca ccgacgaccg cctgcatctc
     1501 cccgcccgcg agggcagcct cttcgacccc gacggctacc ccttcctcga gggccgcgtt
     1561 cgcggccagc ccgacgcccg ccgcccgccc ccccgcgtcc ccgacggcgt gctctggcgc
     1621 attctacaga gcctcctgct cctcgacggc gagcgcctct cctatcgatc gctcgacgtc
     1681 gagcagatcg gcggcgtcta cgagtcgatg atcggcttca agctcgagcg cacaaacggc
     1741 ccgagcatcg ctgtccgccc gcagcacgtc gtcgtcgacc tcgccgacct cctgtcccaa
     1801 aagccagacg cccgcgccaa atttttaaaa gcgcaggcgg gctgcgacct gaccggggcg
     1861 tcgctcgccg ccctcaagtc cgccgacact ccggaggacc tcgtcgccgc cctcggccgc
     1921 aagcagagcc cgcacacccc cgcgatcgtg cccgccggca gcctggttct gcaacccggc
     1981 gaggagcgcc gcaagtccgg atcccactat actccgcgca gcctcaccgg cccgatcgtg
     2041 cgcgccaccc tgcgcccggt cctcgcggct ctcggcgacg ctccgacccc ccagcagatc
     2101 ctcgacctca agctctgcga ccccgccatg ggatccggcg ccttcctcgt cgaggcggcc
     2161 cgccagctcg gcgagcagct gcgccgcgcc tgggagcttc acagggccgc cccgcccatt
     2221 ccccccgacg acgacctcga cctccacgcc cgccgcctcg tcgcccagcg ctgcctgcac
     2281 ggcgtcgacc gcgacccctt cgccgtcgac ctcgccaggc tctcgctgtg gctcgtcacc
     2341 gctgcaaaag accacccctt caccttcgtc gaccacgccc tcaaacacgg cgacagcctc
     2401 gtcggtctga gtaacagcca gatcgccgac tttcgctggg accacgagac gcgcgacgac
     2461 ggcgtcttgt tcgcgcagat ccgccagcaa gtcggcgaag cgagcgccct gcgccgcgag
     2521 gctcacaact acggcgatca ccaggacgcc gccaaacgca gcacattgaa gctcgccgag
     2581 gccgcggtcc accaggcccg cctgatcggc gacgccgcga tcctcgccta cttctcccgg
     2641 gacagcgaca aacaccgcaa cacccgccgc gaggaagttt atagagatac catcatcccc
     2701 gctcaaaaag gcgacccacg agccctcgac cgcctgacgc agctggcggc cgagctgcgc
     2761 ggcggtgacc gccccgtcac cccgtttcac tgggagctcg agttccccga ggtcttcgat
     2821 cgcgaggacc cgggcttcga ctgcttcgtc ggtaaccctc ctttcctcgg cggcaagcgg
     2881 atcagcaccg tgctcggcaa tgtcacgcgt gactggctgg cgcaggccca cgtgaacgcc
     2941 tcgagcaacg ccgacctcgc cgctcacttc tttcgccgcg cctaccacct cctgcgcgcg
     3001 accggcacct tcggcctgat cgccaccaac acgatcacgc agggcgacac cctgcgcgcc
     3061 ggactgtcct atatctgcca cgacggcggc gtcatctacg aggcagacag gcggatccgc
     3121 tggcccggcg ccgcggccgt gatggtctgc gtggtgcatg tcgcccgggc cagagccggg
     3181 gtctcaccgc cccatcgcct cgaccaaagg ccggtcgaca ggatctcggc gttcctgttc
     3241 cacgccggcg gcgacgagat gccggtcccc ttgcggagca ataccgggat cagcttcatc
     3301 ggctgcgaca tcaagggcgc cggcttcacc ttcgatgata ccgtgcccga cgcgaccccg
     3361 gtcgcacgca tgcgcgagct cctcgcgctc gacgcgagca acgccgaggt cgtgcgtccc
     3421 tacatcggcg gcgaggagat caacagcgat cctcgacagg cgccgcatcg atatgtgatc
     3481 catttcggcg agatgcccct cgaagccgcc agcaggtggc ccgcgctgct gcagatcgtc
     3541 gaggagaagg tcaggcccag acgcctcgcc atggcgaggg acctcgcgcg ctggccctgg
     3601 tggcaattct ggcgcgtgcg ggcgcgactg gcggagaaga cccggcacct ctcgtccgtc
     3661 ctggtcaact gccaggtcac cgcgcacctc tcgttcgcct tcctcccgac ccattacgtc
     3721 ttcgcgcaca cggtcaacgt attcggcgtc gaccgacatg gcttcttcgc gatcctccaa
     3781 tctcggtccc acgagatctg ggcccgcttc ttcgggtcgt cgatgaagga cgaccttcgc
     3841 tacaccccca gcgactgctt cgagaccttc cccctcccgc ccgactggca acacgacgcc
     3901 gcgctcgcgc aggccggcaa acgctactac gagctgcgcg ccgacctgat ggtccgcaac
     3961 aacgagggcc tgaccgccat ctacaaccgc ttccacaacc ccgacgagcg ctccccggac
     4021 attctccggt tgcgcgacct ccacgccgaa atggaccgca ccgtgctgac ggcctacggc
     4081 tggcccgacc tcgccgcgcg cgccacctgc gagttccgcc tcgactacga ggacgacaac
     4141 gacgaccccc cgcgcgcccg cacccgaaag aagccctggc gccaccgctg gccccaggcc
     4201 ttccacgacg aggtcctcgc ccgtctgctc gacctcaacc accagcgcgc cagccaggag
     4261 cgcccagccc gcccgcccgc ggcactcctc ccccccgacg acgactgagc ccatcagtga
     4321 ccggacgccc ccataccagg aggtccacgg cgccgagagc gcccagcgtt gcctcagcga
     4381 gccttgccat tacgtcgagt tgcaccaccg atttggcgcg actcgtagtc aattacatca
     4441 gctcgctcgg agaccagttc aatctgctcg acgatgacaa aaggtgaccc gctcgggggc
     4501 tcataacgct tcccaacgat cctggccaga acgcccacgg tcgtgactcc ctcgagcagg
     4561 tccgatgacg cgtgacaggc gatcctatcc ctcggagttc taccgtgaat aagagagcca
     4621 cggtccagat cgatggcgcg aatcagcgtt atttgatcgt agttagacgg ctccatgtcc
     4681 tttgccacgg ccgccatcaa ctttggagcg tgaagcttcc gcagccgaac cggcgcacgc
     4741 gccgtataag tccctccgag cgtcacctct tcgataccgc ccctggggag gactcgcatc
     4801 gtctggacga gggcccgagt ccgcgtctct gcgtccagat ccccaccaaa atcgaggacc
     4861 gcgtcgaagc cttgagcatc ggcacgcgac gccaagtcca tgatctgatc gatcgcttcg
     4921 tggacctttc ccccccggaa gtcgagttgg gcataaggtg cgccgagctc gagccgcgct
     4981 cgcgctggta cgccgttgag tggcgtgatt tgcatggact ccagcgcgaa atccgtggcg
     5041 atctcgatgg gcttctctac ccggcccgtc ctgcctgtgc cgtagcgcgc gagcgaacgc
     5101 atcgcgcgcc gaaaatcctc gtagtagctg gttacggcct gcgtactaag gaactggccg
     5161 ggagcgaatc ggaggtcgat cgccagatag gaccgggaga aaagctcgtc gtcgcttaca
     5221 atcgacgcgg gctccctgag ccgagcgctc acctctcgca agatcgtcac gaacctctgt
     5281 tcgtcttccc cgccgaccgc ctgatatagc ggcagcgggg gctgcccctt gaaccgatcc
     5341 gccagtagcc gcacatattc atcgaggact cgtcgcggat ttgcgccctc gcgcgcgaca
     5401 tctgcggcga gcgcccgaac ctcgtgtagc gtgagaacct gcatctcgat ggcttcgagg
     5461 c
//
LOCUS       DASWNJ010000047         3973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_106915, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000047 DASWNJ010000000
VERSION     DASWNJ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106915"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..751
                     /locus_tag="VGB85_00890"
     CDS             191..751
                     /locus_tag="VGB85_00890"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY0132591.1"
                     /translation="MTAQAAPADTRKILTEAFTHMLRFLRAKRIPAPDCYDVAQEAAL
                     VFLEKLDQIHDPQQSRRFLLGIARMKALQYFERRGRHPSDAFDSHVLPRGTAPTTPSV
                     RLDRKTRLLNVLQALDDEPREAFLLRCEGLKLEEIAAEMKLSLATVKRRLADANAELA
                     RQTRALTPELDDEQRVVAVEAAYRGS"
     gene            complement(736..2286)
                     /locus_tag="VGB85_00895"
     CDS             complement(736..2286)
                     /locus_tag="VGB85_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132592.1"
                     /translation="MHRWPSRAVWLLSLAWGCTPEPPAPGTREAPAAEISAPVAEVAR
                     AEPGRPAWVGTGTVVDALPEAWPEPLRWIASGGGGTPEFNQVSIEQDLALAHERLGPG
                     GLLLFAGGRGSHGVQVQAGRAGDPLIASLAELFAPRGGRDASYRPTTLPVDAAATLEN
                     LRAAIDRGTASEGPPLLLYLAGHGQAGERLADASIELWGQVPLSARELAELLDGRPRP
                     LRVVMTSCFSGGFAELVFTGADASKKPAAPGRCGLFATTAEREASGCDPNPDRAAQEG
                     YGMHFLNALGGRDRAGQPAEIDLDGDGRVSLLEAHARARSASAGIDVPTTTSERWLRA
                     VPRRRGASRGVALPEEDAVISALTRELGLHGEAVGESLRAVEAEMVDASARVVTQGEA
                     EDAAYRRTAAQLLARWPVLDDPWHPEFAAMLASEGPAISAALASSESYLEFLAAGAEL
                     AAAQAEQAELLARSAKLERLVRALETRTLAGELAARGGADWRTYEALLACERWVPGED
                     VKRDQDPR"
     gene            2474..3028
                     /locus_tag="VGB85_00900"
     CDS             2474..3028
                     /locus_tag="VGB85_00900"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY0132593.1"
                     /translation="MQQQPGSNFNPYAPPTADIHQGVQVDQHGQYVLAERGTRLGAVL
                     IDALLVLASALPAGAVLAFMAFAGRDSLSRGESLTMLYGLGGLMGLMVLAFVGYQWYL
                     VATTGQSLAKRWMGIKIVRLDGSPVGFVHGVILRSWVMSALSNIPALGTVVGLVNALM
                     IFGEERRCLHDHIAGTRVIVAPAA"
     gene            3033..3812
                     /locus_tag="VGB85_00905"
     CDS             3033..3812
                     /locus_tag="VGB85_00905"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY0132594.1"
                     /translation="MSQARETRGSARQAPPLDTVAEIETPEHIRFRYQLAGPARRMLA
                     YLIDLLVRVVFVIFVFLILSLSSLLVTDGFSTGAVLVVLFAVEWGYYVLWESLWSGRT
                     PGKRSMNLRVLKEGGYPTGFLDIALRNLLRAADFLPMAYSVGLAVMGLDARFRRLGDM
                     VAGTVVVVEDTGRLGQGLKIHPPPSAAELAALPARPNLSAADLEALDLFLRRVGTLNP
                     AREQELADIIAPVYARRLGVRYDHSARFLALVYLRAIGRTA"
BASE COUNT          534 a         1611 c         1259 g          569 t
ORIGIN      
        1 tgaggtccgg gccgacggcg tctgggccct gcgcgcccgc ggcgacggcg tcgcaagcga
       61 ctggaccctg accggctggg gccccgcgtt cgggcgcttc acggccctcg acggccgggc
      121 ggcgggtccg ctgtccgctt atcgcctgcg ccgctgagcc gttctcacca ccccccggga
      181 cataccgatc atgaccgctc aagccgcgcc cgccgacacc cgcaagatct tgaccgaggc
      241 atttacgcac atgctgcggt tcctgcgggc caagcggatc ccggcgccgg attgttatga
      301 cgtcgcgcag gaggccgcgc tggtgttcct cgaaaagctc gaccagatcc atgacccaca
      361 gcagtcgcgc cggttcctcc tcggcatcgc acgcatgaag gccctgcaat acttcgagcg
      421 tcgcgggcgg cacccgagcg acgccttcga cagccacgtg ctgccccgcg gcaccgcgcc
      481 gaccaccccg agcgtgcgcc tcgatcgcaa gactcgcctg ctcaacgtcc tccaggcgct
      541 cgacgacgag ccgcgcgagg ccttcttgct gcgctgcgag ggcctcaagc tcgaggagat
      601 cgccgcggag atgaagctca gcctcgccac tgtcaaacgt cgactcgccg acgccaacgc
      661 cgagctcgcc cgccagaccc gcgccctcac ccccgagctc gacgacgaac agcgcgtcgt
      721 cgccgtcgag gccgcctacc gcgggtcctg atctcgcttc acatcttctc cgggcaccca
      781 gcgctcgcag gccagcagcg cctcgtacgt ccgccagtcc gcgccgccgc gggccgccag
      841 ctcgcccgcg agcgtgcggg tctcgagcgc tcgcaccagc cgctcgagct tcgcgctgcg
      901 ggccagcagc tccgcctgtt cggcctgcgc cgccgccagc tcggcgcccg cggccaggaa
      961 ctcgagatac gactcgctgc tcgccagcgc cgccgagatc gccggcccct cgctcgccag
     1021 catcgccgcg aactccgggt gccacgggtc gtcgagcact ggccagcgcg cgagcagctg
     1081 cgcggccgtg cggcgatagg ccgcgtcctc ggcctcgccc tgcgtcacca cgcgcgcgct
     1141 cgcatcgacc atctccgcct cgaccgcgcg cagcgactcg ccgaccgcct cgccgtgcaa
     1201 gccgagctca cgcgtcagcg ccgagatcac cgcgtcctcc tcgggcaacg ccacacccct
     1261 gctcgcgccg cgccgtcgcg gcaccgcccg caaccaccgc tccgaggtcg tcgtcggtac
     1321 gtcgatcccc gccgacgccg accgggcacg cgcgtgcgcc tcgagcagcg atactcgacc
     1381 gtcgccgtcg aggtcgatct ccgccggctg gcccgcgcgg tcgcggccgc cgagcgcgtt
     1441 caaaaaatgc atcccgtaac cctcctgcgc cgcgcggtcg gggttcgggt cgcagcccga
     1501 ggcctcgcgc tccgccgtcg tcgcgaacag cccgcaccgc cccggcgccg ctggcttttt
     1561 cgacgcgtcg gccccggtga acaccagctc cgcgaagccg ccggagaagc agctcgtcat
     1621 caccacgcgc agcggacgcg gccgcccgtc gagcagctcc gccagctcgc gcgctgacaa
     1681 cggcacctgg ccccacagct cgatcgacgc gtccgccagt cgctcgccgg cctgcccgtg
     1741 ccccgccagg tacagcagca gcggcggccc ctcgctcgcc gtcccgcggt cgatcgccgc
     1801 gcgcaagttc tcgagcgtcg ccgccgcgtc gaccggcagc gtcgtcgggc gatagctcgc
     1861 gtcacggccc ccgcgcggcg cgaacagctc ggcgagcgag gcgatcagcg ggtcgcccgc
     1921 gcgcccggcc tggacctgca cgccgtggct cccgcgcccg cccgcgaaca gcagcagccc
     1981 gccgggcccg aggcgctcat gcgcgagcgc gaggtcctgc tcgatcgaca cttgattgaa
     2041 ttccggcgtc ccaccgccgc ccgatgcgat ccaccgcagc ggctccggcc aggcctcggg
     2101 cagcgcgtcg acgaccgtgc ctgtcccgac ccaggccggt cgccccggct ccgcgcgcgc
     2161 gacctctgcc accggtgcgg agatctcggc cgcgggcgcc tcccgggtcc ctggagccgg
     2221 cggctcgggc gtgcagcccc acgcgagcga cagcagccac acagcgcgac tcggccagcg
     2281 gtgcaccacg cgaggatacg gcgcacgcgg cagcgagtga agcgaattcg catgactccg
     2341 ttcgcaacct ggtcaaagag acatgtcgca acctcttcgc gcccgtccgg cgccgcgcca
     2401 ccccgcggtg gttgacaccg atggccccgt catggtgcgc tggctcccgc gtagaagcag
     2461 gtccaaagat cccatgcagc agcagcccgg ctcaaatttc aatccgtacg cccctccgac
     2521 cgccgacatc catcagggcg tgcaggtcga ccaacacggt cagtatgtcc tcgccgaacg
     2581 cggcacgcgc ctcggcgccg tgctgatcga cgcgctgctc gtgctggcct ccgcgctgcc
     2641 cgccggggcc gtgctcgcct tcatggcctt cgccggccgc gacagcctga gccgcggcga
     2701 gagcctgacg atgctctacg ggctcggcgg tctcatgggg ctgatggtcc tcgcgttcgt
     2761 ggggtaccag tggtacctcg tcgcgacgac cggccagtcg ctcgccaagc gctggatggg
     2821 catcaagatc gttcgcctcg atggttcgcc ggtcggcttc gtgcacggcg tcatcctgcg
     2881 cagctgggtg atgagcgcgc tgtccaacat cccggcgctc ggcacggtcg tcggcctcgt
     2941 caacgcgctg atgatcttcg gcgaggagcg ccgctgtctg cacgaccaca tcgccggcac
     3001 ccgcgtgatc gtggcccccg ccgcgtaagc gcatgagtca ggcccgcgag acccggggga
     3061 gcgcccgcca ggcccccccg ctcgacaccg tcgccgagat cgagaccccg gagcacatcc
     3121 ggtttcgcta ccagctcgcc gggccggcgc gccgcatgct cgcgtatctg atcgacctgc
     3181 tggtccgcgt cgtcttcgtc atcttcgtgt tcctgatcct ctcgctcagc agcctgctcg
     3241 tcaccgacgg cttctcgacc ggcgccgtgc tcgtcgtcct gttcgccgtc gagtggggct
     3301 actacgtgct gtgggagagc ctgtggagcg gcaggacccc cggcaagcgg tcgatgaacc
     3361 tgcgcgtgct caaggagggc ggctatccga ccggcttcct cgacatcgcg ctgcgtaact
     3421 tgctccgcgc cgccgacttc ctgccgatgg cctacagcgt cggcctcgcg gtgatgggcc
     3481 tcgacgcccg ctttcgccgc cttggtgaca tggtcgctgg caccgtcgtc gtcgtcgagg
     3541 acaccggccg gctcggccag ggcctcaaga tccacccgcc gccgtccgcc gccgagctcg
     3601 cagccttgcc cgcgcgcccg aacctcagcg ccgccgacct cgaggcgctc gacctgttcc
     3661 tgcgccgcgt cggcaccctc aaccccgcgc gcgagcaaga gctcgccgac atcatcgccc
     3721 ccgtgtacgc tcgccgcctc ggcgtgcgct acgaccactc cgcccgcttc ctcgcgctcg
     3781 tgtacctgcg cgcgatcggc cgtaccgcct gacctgtccc caaagccatg cgatcgtgga
     3841 cacaggatga gttcgtcgcg cgccgccagg ccgagtggac cgcgctcggc gacatcctga
     3901 gcagcggcaa gcccctgcac aaactcccgc ccgccgacat ctcgcgcttc tcggccctct
     3961 accgcggcgc ctg
//
LOCUS       DASWNJ010000048         3484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_142270, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000048 DASWNJ010000000
VERSION     DASWNJ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142270"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="VGB85_00910"
     CDS             <1..873
                     /locus_tag="VGB85_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132595.1"
                     /translation="KFLATVALGAGATTLPVWLSRAFRRERDCEVDLADHLADSPVQM
                     SDPEQCLPGGEHGPQKPRLVFVIPTDDRLQWLRGQAFGELLNHGSDAQLAPLACFEVL
                     CRPTGDPLDEPLMLLLDPATGTTISLDAPLPVDPDDPYPPPTRDEEARIDDRIAVLAR
                     LISDAADGSRLAAQACRERASLPPGDLRRLDELPASIGELQPSEVDRAPATTMLAARS
                     AEPEVREHLTALLAAGVRARLCDRPIDGAPWARATGCGVRVEGDDRPSGIMCGMGHTP
                     ERSRRFLKFYVDQD"
     gene            877..2139
                     /locus_tag="VGB85_00915"
     CDS             877..2139
                     /locus_tag="VGB85_00915"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEY0132596.1"
                     /translation="MTAPLVGLALHPEVTYLERVMPLLEREVEVLEVGPETTWALDDA
                     GALVPNDYHRRFLALGERLGLPFIAHGVGLSPGSAADAPRRARWLARMAEDQQKFNYR
                     WYTDHLGATVLDGLEMTLPLGLPMTAEVAEIVRARLCEMQRVVPRVGLENSAIYFVLG
                     EALAEPHFINTILGGEHHLLLDLHNLYTMAHNGGFDPHAYLARLDLSRVIEIHLSGGS
                     DSDPRWLPSRRSLRVDSHDDGVPEPVWDLFAQVLPRCPRLGAVIVERMEDTLGPGEVH
                     ALAGELRRARSIVHALHRSTPAPDPVACPMPALDLRGLAAQLEHERSLAAVLRATDPV
                     AALACAPAELQRTLAGIDPDGLRLAALFITRLRFERVQHGDLAAGRHFDDDPAGFAAE
                     FRRYLAEVPPTAAFPQDEGDLYRRWRAR"
     gene            complement(2162..>3484)
                     /locus_tag="VGB85_00920"
     CDS             complement(2162..>3484)
                     /locus_tag="VGB85_00920"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0132597.1"
                     /translation="DAPPPEQAPQIAAVRETLAEARSLQAAGKYKESVALAIGADAAA
                     DALDYTPLVAEAKLVHGRSRWQAGEHEAAIPVLSAAAASALASRDDARLLTAFVDLSH
                     VLGYSLGRDKEAAAWINLAEGALRRSGDRPRDRARVLGARALIDVAANRYAEAEVALL
                     RQLELLETEYGPDSPHLGRTINALGGVYLRTGRYVEAETLFVRAVELEEAVGGPLHPD
                     VALPLNNLALSHERQGRRLDAVVALRRAKTIFERTAGVDHPNVGILQQNIGGMLRLAG
                     KPAEARVELDAARVLLEAKLGPDHPAIAGVYTFSGDVAIDEGDLARARADYQRADDLR
                     SKALGADHPDRSLALLGLGKVALAEGKPDAAVAPLELALKLWTGTTPDPQDIGEVKWN
                     LARALWVADEARARGLIAEAQGHFTEAGINSAGLIKEIEAWLAKHPAN"
BASE COUNT          434 a         1441 c         1118 g          491 t
ORIGIN      
        1 aagtttctcg caacggtcgc tctcggcgca ggcgcgacca ccctccccgt gtggttgtct
       61 cgcgcgtttc gccgcgagcg cgactgcgag gtcgacctcg ccgaccacct cgccgattcg
      121 ccggtgcaga tgtcggaccc cgagcagtgc ctgcccggcg gcgagcacgg cccgcaaaag
      181 ccccgactcg tgttcgtcat ccccacggac gatcggctcc agtggctgcg cggccaggcc
      241 ttcggcgagc tgctcaacca cggcagcgac gcccagctcg ccccgctcgc ctgcttcgag
      301 gtcctgtgcc gcccgaccgg cgaccccctc gacgagccgc tgatgctgct gctcgaccct
      361 gccacgggca ccacgatctc tctcgacgcc ccgctgcccg tcgacccgga cgacccctac
      421 ccgccgccga cccgcgacga ggaggcccgc atcgacgacc gcatcgccgt gctcgcccgc
      481 ctgatctccg acgccgccga tggctcccgg ctggccgccc aggcctgccg cgagcgcgcc
      541 agcctgccac cgggcgacct gcgccgcctc gacgagctgc ccgccagcat cggcgagctg
      601 caaccatccg aagtcgaccg cgcccccgcg accaccatgc tcgcggcccg cagcgccgag
      661 cccgaggtcc gcgagcacct caccgccctg ctcgccgccg gagtacgtgc ccgcctgtgc
      721 gaccgcccga tcgacggcgc cccgtgggcc cgcgcgaccg gctgcggggt cagggtcgag
      781 ggcgacgacc gcccctcggg catcatgtgc ggcatgggcc acacccccga gcgcagccgt
      841 cgcttcctca agttctacgt cgaccaggac tgaccgatga ccgcgcccct cgtcggcctc
      901 gcgctgcacc cggaggtcac ctacctcgag cgcgtgatgc cgctgctcga gcgcgaggtc
      961 gaggtcctcg aggtcggccc cgagaccacc tgggccctcg acgacgctgg cgcgctggtc
     1021 cccaacgact atcaccgccg cttcctggcg ctcggtgagc gcctcggcct gcccttcatc
     1081 gcccacggcg tcggcctgtc ccccggctcc gccgccgacg ccccgcgacg cgcccgctgg
     1141 ctcgcgcgca tggccgagga ccagcaaaag ttcaactatc gctggtacac cgaccacctc
     1201 ggcgccaccg tgctcgacgg cctcgagatg accctcccgc tcggcctgcc gatgaccgcc
     1261 gaggtcgccg agatcgtgcg cgcccgcctg tgcgagatgc agcgcgtcgt cccccgcgtc
     1321 ggcctcgaga acagcgcgat ctacttcgtg ctcggcgaag cgctcgccga gcctcacttc
     1381 atcaacacga tcctcggcgg cgagcaccac ctcctgctcg acctccacaa cctgtacacg
     1441 atggcccaca acggcggctt cgatccgcac gcgtacctgg cccgcctcga cctctcgcgc
     1501 gtgatcgaga tccatctgtc cggcggcagc gacagcgacc cccgctggct gccctcgcgt
     1561 cgcagcctgc gcgtcgacag ccacgacgac ggcgtcccgg agcccgtgtg ggacctgttc
     1621 gcgcaggtgc tgccgcgctg cccgcggctc ggcgccgtga tcgtcgagcg catggaagac
     1681 acgctcggcc ccggcgaagt gcatgcgctc gccggcgagc tacgccgcgc gaggtcgatc
     1741 gtccatgcgc tgcaccgttc cacccccgcg ccggatcccg tcgcgtgccc gatgcccgcc
     1801 ctcgacctcc gagggctcgc cgcccaactc gagcacgagc gctcgctcgc cgcggtgctg
     1861 cgcgccacgg acccggtcgc cgccctcgca tgtgcgccgg ccgaactgca gcgcacgctc
     1921 gccggcatcg accccgacgg cctgcgcctc gccgcgctgt tcatcacccg cctgcgcttc
     1981 gagcgggtcc agcacggtga cctcgcggcc gggcgtcact tcgacgacga ccccgccggc
     2041 ttcgccgccg agtttcgccg ctacctcgcc gaggtcccgc cgacggccgc cttcccacag
     2101 gacgagggcg acctgtaccg ccgctggcgc gcccgatgac gcacctgtcc ttcgggacag
     2161 ctcagttcgc cggatgcttc gccagccacg cttcgatctc ttttattaag cctgcggagt
     2221 tgatgcctgc ctcggtgaag tggccctgcg cctccgcgat caacccgcgc gcccgcgcct
     2281 cgtcggcgac ccacagcgcg cgcgccaggt tccacttgac ctcgccgatg tcctgcgggt
     2341 ctggcgtcgt gccggtccac agcttcaatg ccagctccag cggcgcgacc gcggcgtccg
     2401 gcttgccctc ggcgagcgcg accttgccga gcccgagcag cgccagcgag cggtcggggt
     2461 ggtcggcccc gagcgccttg ctgcgcaggt cgtccgcccg ctgatagtcc gcccgcgcgc
     2521 gcgccaggtc gccctcgtcg atcgcgacgt caccgctgaa tgtatacaca ccggcgatcg
     2581 ccgggtggtc cggtccgagc ttcgcctcga gcagcacccg cgccgcgtcg agctcgaccc
     2641 gcgcctccgc cggcttgccc gcgaggcgca gcatgccgcc gatgttctgc tgcaaaatgc
     2701 cgacgttggg gtggtctacc ccggcggtgc gctcgaagat cgtcttggcc cggcgcagcg
     2761 cgaccacggc gtcgagccgc cgaccctgac gctcgtggct cagcgcgagg ttgttgagcg
     2821 gcagcgcgac gtccgggtgc agcggcccgc cgacggcctc ctccagctcg accgcgcgga
     2881 cgaacagcgt ctcggcctcg acgtaacgcc cggtgcgcag gtacacgccg ccgagcgcgt
     2941 tgatcgtcct gccgaggtgc gggctgtccg ggccgtactc cgtctccagc agctcgagct
     3001 ggcgcagcag cgccacctcg gcctccgcgt agcgattggc ggcgacgtcg atcagggccc
     3061 gcgcgccgag cacccgcgcc cgatcccgcg gccggtcgcc gctgcgacgc agcgcgccct
     3121 cggccaggtt aatccacgcc gccgcctcct tgtcgcgccc gaggctgtag ccgaggacgt
     3181 gcgagagatc gacgaaggcg gtcagcaggc gcgcgtcgtc gcggctcgct agcgccgacg
     3241 cggccgcggc cgacagcacc gggatcgccg cctcgtgctc gccggcctgc cacctgctgc
     3301 ggccgtgcac cagcttcgcc tcggccacca gcggcgtgta gtcgagggcg tccgccgccg
     3361 cgtccgcgcc gatcgccagc gcgaccgatt ccttgtactt gcccgccgcc tgcagcgagc
     3421 gcgcctccgc cagcgtctcg cgcaccgccg cgatctgcgg ggcctgctcc ggcggcgggg
     3481 cgtc
//
LOCUS       DASWNJ010000049         4178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_142278, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000049 DASWNJ010000000
VERSION     DASWNJ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142278"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1977
                     /locus_tag="VGB85_00925"
     CDS             <1..1977
                     /locus_tag="VGB85_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132598.1"
                     /translation="ALRTQALALTGEPAAIRLPGALAGALLVGLATLLARQLGAPPRF
                     ALLAGGFALAMPLLQSQSRLALGAPLTELAATTAALLGAAALAHARRGPALALGLATL
                     VALALGLLCGGLVPGVLLPLLAIAIAWPQGHARPDMSLAPGAPSRWLAAALALAIVGV
                     LALTAALAYHQHDGFIAALGSARDLALGERPHTRPFTATLEQLSDQIFPWLPLLVVGL
                     LNPGRARWPALWLLAGLVLISAWSLRYGPQPVPLTVPVAVVCAAAVEHLLKARTGARR
                     LGLLIVFAGGLILAKDARRTPSHVGAVLVHGKGEQSYPARELDAPDTLAGLAYLAMFG
                     LLLAVLVNRPTIVQTRLDRLRARLPLALPVGVLLAVLGHQALRYGHDLLPRTSEALSL
                     HRPLTRFARWHAAGDLPGPLLIHRISDPGVALYGPPDTDRSVVGSRDEIVQSFKLDAP
                     CAALIRSSELAALYQQAREAKWPLYVLDASNRDVVLVSNVLPPGASDENPILDVLRDE
                     PPVLANPTFLRFEDNLEVLGWAWHEPVVRGRETTLEVALRVLKPLPSGSKIYVRLQQG
                     KLSRINPAAHELAGGVYPPQHWRAGDYILHRFTVAVPMLEVLPGAHEVVLGLRRTESS
                     NFKISEPLTDDNPHGVTFRGPTHEFADVGAVQVW"
     gene            1996..3405
                     /gene="xseA"
                     /locus_tag="VGB85_00930"
     CDS             1996..3405
                     /gene="xseA"
                     /locus_tag="VGB85_00930"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY0132599.1"
                     /translation="MATPTPKIYTVAELVNRAHRCIEQNFNGVHVEGEVTNLRVVGSG
                     HAYFTLRDAEAILPVAMWRSALARLKFKLQDGQRLRVYGRPGIYAAQGKFQLYAERAE
                     PAGQGALLLQLEQLKARLAAEGLFARERKRPLPRWPRVIGVVTSPTGAAIHDILKVAR
                     RRCPVRILLAPALVQGDDAPASIIRALTRLQAMPEVEVIIVGRGGGASEDLWAFNDEG
                     VARAVAACRVPVVSAVGHEVDVTICDHVADLRAATPSHAAELVVPDRDGCLTQLHQLR
                     RRLGMATHRHILNEQSRHERATHRLQVAARRLIYAPRRDFAALVARLEAVHPRRRLDR
                     DRKRLTALLARLQQQHPRNRLAELRRRLAAADSRLQQQHPRHRLAQLRRRLAAAELTL
                     QQRAGQLSQPARLRLARAAGALHALSPLAVLERGYAVLRDPTGTVVRAADQVQVGDEL
                     SVRLHRGTLRARVVARDEP"
     gene            3511..3873
                     /locus_tag="VGB85_00935"
     CDS             3511..3873
                     /locus_tag="VGB85_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132600.1"
                     /translation="MDRLSHLLATLRVVLVAVSVCLLGAASRTEHCIDRSTSELQTGH
                     AQADDFDFPEPSPVVAQERDEDPRGGTEDDAAHGSHGGLAWLTALATGIHMIRGPPPA
                     PRGVTWRPRSSRGPPAQV"
     gene            3968..>4178
                     /locus_tag="VGB85_00940"
     CDS             3968..>4178
                     /locus_tag="VGB85_00940"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEY0132601.1"
                     /translation="MFDPNVPKDGLAGFKEHLRADLISGFLVFLIALPLCIGISMASG
                     FGPVGGLFTAIIGGVLVSFFSGAPIT"
BASE COUNT          505 a         1805 c         1282 g          586 t
ORIGIN      
        1 gccctgcgga cgcaggccct cgcgctcacc ggcgagcccg ccgcgatccg cctgcccggc
       61 gccctcgccg gcgcgctgct ggtcggcctc gccaccctcc tcgcccgcca gctcggcgcc
      121 ccgccgcgct tcgccctgct cgccggcggc ttcgcgctcg ccatgcccct gctccagagc
      181 cagtcgcggc tcgccctcgg cgccccgctc accgagctcg ccgcgaccac ggccgccctg
      241 ctcggcgccg ccgccctcgc ccacgcccga cgcggcccgg ccctcgccct cggcctcgcc
      301 accctcgtcg ccctcgcgct cggcctgctg tgcggcggcc tcgtccccgg cgtcctcctg
      361 ccgctgctgg cgatcgcgat cgcctggccg caaggacatg cccgcccgga catgtccctt
      421 gcgccaggtg ccccgtcgcg ctggctcgcc gccgccctcg cgctggcgat cgtcggcgtg
      481 ctcgccctca ccgccgcgct cgcctaccac caacacgacg gcttcatcgc cgccctcggc
      541 tccgcgcgcg acctcgccct cggcgaacgc ccgcacacgc gccccttcac cgccacgctc
      601 gagcagctca gcgaccagat cttcccgtgg ctgcccttgc tcgtcgtcgg cctgctcaac
      661 cccggccgcg cccgctggcc cgccctgtgg ctgctcgccg gcctcgtgct gatctccgcg
      721 tggtcgctgc gttacggccc gcagccggtc ccgctgaccg tgccggtcgc cgtcgtctgc
      781 gccgccgccg tcgaacacct gttaaaagcc cgcaccggcg cgcgcaggct cggcctgctc
      841 atcgtcttcg ccggcggcct gatcctcgcc aaggacgccc gccgcacccc gagccacgtc
      901 ggcgccgtgc tcgtccacgg caagggcgag cagagctacc ccgcgcgcga gctcgacgcc
      961 cccgacacgc tcgccggcct cgcctatctg gcgatgttcg gcctcctgct cgccgtgctc
     1021 gtcaaccgcc cgacgatcgt tcaaacccgc ctcgatcgcc tgcgcgcccg cctcccgctc
     1081 gccttgccgg tcggcgttct gctcgccgtg ctcggccacc aggcgctgcg ctacggccac
     1141 gacctgctcc cgcgcacctc cgaggctctc tcgttgcacc gcccgctgac gcgcttcgct
     1201 cgctggcacg ccgccggcga cctgcccggc ccgctgctga tccaccgcat cagcgacccc
     1261 ggcgtcgccc tctacggccc cccggacacc gaccgcagcg tcgtcggctc gcgcgacgag
     1321 atcgtccagt cgttcaagct cgacgccccc tgcgccgccc tgatccgcag cagcgagctc
     1381 gccgccctgt atcagcaggc gcgcgaggcg aagtggccgc tgtacgtgct cgacgccagc
     1441 aaccgcgacg tcgtgctcgt cagcaacgtg ctcccgcccg gcgccagcga cgagaacccg
     1501 atcctcgacg tcctgcgcga cgagccaccg gtcctcgcca acccgacctt tttgcgcttc
     1561 gaggacaacc tcgaggtcct cggctgggcc tggcacgagc cggtcgtgcg cggccgcgag
     1621 accaccctcg aggtcgccct gcgcgtcctc aagccgctcc cgagcggcag caagatctac
     1681 gtgcgcctgc aacagggcaa gctctcgcgc atcaaccccg ccgcccacga gctcgccggc
     1741 ggcgtgtacc cgccgcagca ctggcgcgcc ggcgactaca tcctgcaccg cttcaccgtc
     1801 gccgtgccga tgctcgaggt cctgcccggc gcgcacgagg tcgtgctcgg cctgcgccgc
     1861 accgagtcga gcaacttcaa gatctccgag cccctgaccg acgacaaccc gcacggcgtc
     1921 accttccgcg gccccacaca cgagttcgcc gacgtcggcg ccgtgcaggt ctggtgagcg
     1981 tgctataagc cccgcgtggc gacgccgacg ccgaagatct acaccgtcgc ggagctcgtc
     2041 aaccgcgccc accgctgcat cgagcagaac ttcaacggcg tgcacgtcga gggcgaggtc
     2101 accaacctgc gcgtcgtcgg cagcggccac gcctacttca ccctgcgcga cgccgaggcg
     2161 atcctcccgg tcgcgatgtg gcggtctgcg ctggcccgcc tcaagttcaa gctgcaggac
     2221 ggccagcgcc tgcgcgtcta cggccgcccc ggcatctacg ccgcgcaggg caagttccag
     2281 ctgtacgccg agcgggccga gcccgccggt cagggcgcgc tgctgctcca gctcgagcag
     2341 ctcaaggcgc gcctcgccgc cgagggtctg ttcgcccgcg agcgcaagcg gccgctgccg
     2401 cgctggccgc gggtgatcgg cgtcgtcacc tcgccgaccg gcgccgcgat ccacgacatc
     2461 ctcaaggtcg cccgccgccg ctgcccggtc cggatcctgc tcgcgccggc cctcgtccag
     2521 ggtgacgacg cccccgccag catcatccgc gcgctgactc gcctgcaggc gatgcccgag
     2581 gtcgaggtga tcatcgtcgg ccgcggcggc ggagccagcg aggacctgtg ggccttcaac
     2641 gacgagggcg tcgcccgggc cgtcgccgcc tgtcgcgtgc cggtcgtgtc cgccgtcggc
     2701 cacgaggtcg atgtcacgat ctgcgaccac gtcgccgacc tgcgcgccgc caccccctcg
     2761 cacgccgccg agctcgtcgt gcccgaccgc gacggctgcc tgacccagct gcatcagtta
     2821 cgccgccgcc tcggcatggc gacccaccgc cacatcctca acgagcagag ccgccacgag
     2881 cgcgcgaccc accgcctcca ggtcgccgcg cgccgcctga tctacgcccc gcgtcgcgac
     2941 ttcgctgcgc tcgtcgcccg cctcgaggcc gtgcacccgc gccgtcgcct cgaccgcgac
     3001 cgcaagcgcc tcaccgccct gctcgcccgc ctgcaacagc agcacccgcg caaccgcctc
     3061 gccgagctcc gacgccgcct cgccgccgcc gactcgcgcc tgcaacaaca acacccgcgc
     3121 caccgcctcg cccagctgcg ccgccgcctc gccgccgccg agctcaccct gcaacagcgc
     3181 gccggccagc tcagccagcc cgcccgcctc cgcctcgcgc gggcggcggg cgccctccat
     3241 gcgctcagcc cgctggccgt gctcgagcgc ggctacgcgg tgctgcgcga ccccaccggc
     3301 acggtcgtgc gcgccgcgga ccaggtccag gtcggggacg agctgtcggt ccgcctgcat
     3361 cgcggcactt tgcgcgcccg cgtcgtcgcc cgcgacgagc cgtgaacccc ggggatgcct
     3421 tcgatcgcgg gccagtgctc ggatctacga cgcaagattc gcgcactatt tggcttgaac
     3481 gcaatttcgg cagttctata atcgccgccg atggaccggc tgagccacct gctcgcgacg
     3541 ctgcgagtgg tgctcgtggc ggtgagcgtg tgcctgctgg gcgcggcctc gcggaccgag
     3601 cattgcatcg atcgatcgac ctccgagctg cagaccgggc acgcccaggc cgacgacttc
     3661 gacttccccg agccgagccc ggtcgtcgcc caggagcgtg acgaggaccc ccgcgggggc
     3721 accgaggacg acgccgccca cggttcgcac ggtggcctcg cctggctgac ggccctcgcg
     3781 accggcatcc acatgatccg cggccccccg cccgcgccgc gcggcgtgac gtggcgtccg
     3841 aggagctcgc gcggcccccc ggcgcaggtc tgagcggtcc ccccgtcttc agaactgtcc
     3901 gcgagaacag cctcgacctc ctgacctgtg tctgtcccgc gcccgcggga cccgaaaaag
     3961 agtccccatg ttcgacccca acgtccccaa ggacggcctc gccggcttca aagagcacct
     4021 ccgtgccgac ctgatctccg gcttcctcgt gttcctcatc gccctcccgc tgtgcatcgg
     4081 catctcgatg gccagcggct tcggccccgt cggcggcctg ttcacggcga tcatcggcgg
     4141 cgtcctcgtc agcttcttct cgggcgcccc gatcacga
//
LOCUS       DASWNJ010000050         4359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_354227, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000050 DASWNJ010000000
VERSION     DASWNJ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354227"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VGB85_00945"
     CDS             <1..418
                     /locus_tag="VGB85_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132602.1"
                     /translation="DRYIAWLSDANESPSQRINHGAEYDDTPYLLRTGVQVAANFDDL
                     ANNGPWPGIDITDKYESIANVQVWTNTAIDGTSFSDQNHCEEWGSDSGDHTARTGMNR
                     MAEDSPDIEIWQQFGFWTSWTPMTCDAKYRLYCFEN"
     gene            complement(425..1087)
                     /locus_tag="VGB85_00950"
     CDS             complement(425..1087)
                     /locus_tag="VGB85_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132603.1"
                     /translation="MLKSSATPIPRLTLHAEGPASLVLRPDHLRVEINVWVLDLEVNR
                     AIALIEPAIEAAMRELTGVLPGARVLLRGLELRRNVDKTSRANHELAAVACDGHIHVP
                     LAAEASYWPRAHAVASLHATLAAAAAAGSKLKHPLHLGFGPALALVDDPEQHRLPLIS
                     RWLARARELATQAQAQNLCGALHLGECTVPGPVTQHPRSLEEVELRLALDAPLIATPA
                     DE"
     gene            1355..2299
                     /locus_tag="VGB85_00955"
     CDS             1355..2299
                     /locus_tag="VGB85_00955"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY0132604.1"
                     /translation="MSERSDLSNSSVYAPISSVAELLAAAAGEGLRLSTSAADFDDTG
                     LDFRVVEAVDRDGTTWIVRTPRRADVVAAARVEARALQLVRPRLPVAVPDWRIHSDAV
                     IAYPRLAGAPAVTMTTAGPRWQVVDPAAPCETFLADYAAALAALQAVPVAEVAAAGVP
                     VRSRDLVRARVRGDLEVTRDALAPTPAVWRRWQRWLTDHARWPGHVALVHGDLHPGHL
                     LLADDGHLVGVLDWTEAHVGDPATDLALFFGCFGAAALADVIDRFAAAGGRVWPGLAE
                     HAAERWAAFPALCAAWALRHGNAGMLEHARAMLATVES"
     gene            complement(2345..3496)
                     /locus_tag="VGB85_00960"
     CDS             complement(2345..3496)
                     /locus_tag="VGB85_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0132605.1"
                     /translation="MSSTYEQLFQLSQALLATLDTGGCFLDINPAWERTLGWTVDELR
                     ALRPLELVHPDDLETTLAIGSGLVGGAGQIHLETRFRCKDGRYKWLMFASQVETDSGG
                     APQKYFVTAVDVTPFKEALREREEAASQLHLFKVMLENTPDQVGIVDLEGRVRFRNSA
                     ALRHIESIGRGGDHVTAAHVHSPEFLEKLDREVTPELQATGVWSGEGDLLRPDGSVMP
                     TYMILVALRDARGEVVAFGTLIRDVTPQKQLELQLRGAVQALATPMIPISDRVLVMPL
                     IGQMDSERTAQVTRAALEGVHGRGIEVVILDVTGLQNIDTQVGGALLHTAHALRLLGV
                     RTVLTGIQPSVAQALIGLGLDLTAVETASTLQRAISLALSSDSRPRRRG"
     gene            complement(3717..>4359)
                     /locus_tag="VGB85_00965"
     CDS             complement(3717..>4359)
                     /locus_tag="VGB85_00965"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0132606.1"
                     /translation="ALGHLGGALLALGRHDEARVTFERALALRRQAIGAEDPAINGIL
                     AGLGGTLAALGEHDEAIPILERALSLREELGVDKFHLAPILNNLGRSLRQLGRHEEAR
                     RMHERALALQEAALGPSHANVAASLLGLGELALARGEPALALPLLERALTLHSVEDHA
                     NVQLALAEALWPSEQDRPRARALATAAQEHWRGLGHQPNLARATAWLATHPGV"
BASE COUNT          568 a         1519 c         1643 g          629 t
ORIGIN      
        1 cgacaggtac atcgcctggc tcagcgacgc gaacgagtcg ccttcgcagc gaattaacca
       61 tggcgcagag tatgacgata ccccctacct gctgcgtaca ggcgtgcaag tcgccgcgaa
      121 ctttgacgat ctcgcgaaca acgggccgtg gcccggtatc gacatcacgg acaaatacga
      181 gtcgatcgcc aacgtgcagg tctggacgaa caccgcgatc gacggcacca gcttcagcga
      241 ccagaaccac tgcgaggagt ggggctccga ctccggggat cacaccgcgc ggaccggtat
      301 gaaccggatg gcagaggact ctccggacat cgagatctgg cagcagttcg gcttctggac
      361 cagttggacc cccatgacct gcgacgcgaa gtaccggctg tactgcttcg aaaattgacg
      421 gcgcttactc gtccgcgggc gttgcgatca gcggggcgtc gagggcgagg cggagctcga
      481 cctcttcgag gctgcgcggg tgttgggtga cggggccggg gaccgtgcat tcgcccaggt
      541 gcagggcgcc gcacaggttc tgcgcctggg cctgcgtggc gagctcgcgg gcgcgggcca
      601 gccagcggga gatcagcggt aggcggtgct gctcggggtc gtcgacgagg gcgagggcgg
      661 ggccgaagcc gaggtgcagc gggtgcttga gcttgctgcc ggcggccgcg gccgccgcga
      721 gggtcgcgtg caggctggcg acggcgtggg cgcgtggcca gtagctagcc tcggccgcga
      781 gcgggacgtg gatgtggccg tcgcacgcga cggcggcgag ctcgtggttg gcgcggctgg
      841 tcttgtcgac gttgcggcgc agctcgaggc cccgcagcag cacgcgcgcg ccgggcagta
      901 cgccggtaag ctcgcgcatg gcggcctcga tcgccggttc gatcagggcg atcgcgcggt
      961 tgacctcgag gtcgagcacc cacacgttga tctcgacgcg cagatggtcg gggcgcagca
     1021 ccagcgaggc ggggccctcg gcgtggagcg tcaggcgggg gatgggcgtg gcgctggact
     1081 tgagcattgc gcatcgtagg cccgggatcc gcggggcgcg aggggtcgtg gtgatcgatg
     1141 ccgcccggtt gcaggcgcgc gcgacccggc atacaactgc gtggccatgt gcatcgccca
     1201 cattcgactc cgctgagcgc cggcaggccg cgtgtgtctg ccgtgagacc cgtgcccgtc
     1261 ccctggcgag atccggcgcg ggcgcctcga catgtactga catgctcgat gtgcgccggc
     1321 gcgggaccgc ctcgaacctc cgaggagtcc gaacatgtcc gagcgatccg acctctccaa
     1381 ttcttctgtg tacgcgccga tctcgagcgt cgccgagctg ctcgcagccg ccgctggcga
     1441 gggcctgcgg ctgagcacca gcgcggccga cttcgacgac accggcctcg actttcgcgt
     1501 ggtcgaggcc gtcgaccgcg atggcacgac gtggatcgtc cgcaccccgc ggcgggccga
     1561 cgtcgtcgcc gcggcccgcg tcgaggcgcg ggcgctgcaa ctggtgcggc cgcgcctgcc
     1621 ggtcgccgtg cccgactggc ggatccacag cgacgccgtc atcgcctatc cgcggctcgc
     1681 gggggcgccc gcggtcacga tgaccaccgc ggggccgcgc tggcaggtcg tcgatcccgc
     1741 ggccccctgc gagacctttt tggccgacta tgccgcggcg ctcgcggccc tgcaggcggt
     1801 gccagtcgcc gaggtcgcgg ccgccggtgt gccggtgcgg agccgcgacc tggtacgcgc
     1861 gcgggtccgc ggtgacctcg aggtcacgcg cgacgcgctc gcgcccacac ccgcggtgtg
     1921 gcgtcgctgg cagcgctggc tcaccgacca cgcgcgctgg cccgggcacg tcgcgctcgt
     1981 gcacggcgac ctgcacccgg gccacctcct gctcgccgac gacggtcacc tcgtcggggt
     2041 gctcgactgg accgaggcgc acgtcggcga ccctgcgacc gacctggcgc tgttcttcgg
     2101 ctgcttcggc gccgcggcgc tcgccgacgt catcgaccgc ttcgccgccg cggggggccg
     2161 cgtctggccg ggcctcgccg agcacgcggc ggagcgctgg gcggcgttcc cggcgctgtg
     2221 cgccgcgtgg gcgctgcgcc acggcaacgc gggcatgctc gagcacgccc gcgcgatgct
     2281 cgcgaccgtc gagtcgtgac gcacgggccc ggcgcacggg gtgacgccgt gcgccggggt
     2341 cgagctagcc gcgtctgcgc gggcggctgt cgctcgacag cgcgagggag atcgcgcgtt
     2401 gcagggtgct cgccgtctcg accgcggtga ggtcgaggcc gaggccgatc agcgcctgcg
     2461 cgacggacgg ctggatcccc gtcaggaccg tgcgtacgcc gagcagccgc agggcgtggg
     2521 ccgtgtgcag cagcgcgccg ccgacctgcg tgtcgatgtt ctgcaggccg gtgacgtcga
     2581 ggatcacgac ctcgatgccg cggccgtgca cgccctcgag cgccgcgcgg gtgacctggg
     2641 ccgtgcgctc gctgtccatc tggccgatca gcggcatcac gaggacgcgg tccgagatcg
     2701 ggatcatcgg ggtcgccagc gcctggacgg ccccgcgaag ctgcagctcg agctgctttt
     2761 gcggggtcac gtcgcggatc aacgtgccga acgcgacgac ctcgccgcgc gcgtcgcgga
     2821 gcgcgacgag gatcatgtag gtcggcatca ccgagccgtc ggggcgcagc aggtcgccct
     2881 cgccgctcca cacgcccgtg gcctgcagct ccggggtgac ctcgcggtcg agcttctcga
     2941 ggaactcggg gctgtgcacg tgcgccgcgg tcacgtggtc cccgccgcgt ccgatcgact
     3001 cgatgtggcg cagcgccgcg gagttacgga agcggacgcg gccctcgagg tcgacgatgc
     3061 cgacctggtc cggcgtgttc tccagcatca ccttgaacag gtgcagctgc gaggcggcct
     3121 cctcgcgctc gcgcagcgct tccttgaagg gcgtgacgtc gaccgcggtc acgaagtatt
     3181 tttgcggcgc gccgccgctg tcggtctcga cctgtgaggc gaacatcagc cacttgtagc
     3241 gcccgtcctt gcagcgaaag cgggtctcga ggtggatctg gccggcgccg ccgaccagcc
     3301 cggagccgat cgcgagcgtg gtctcgaggt cgtcggggtg cacgagctcg agcggccgca
     3361 acgcgcgtag ctcgtcgacc gtccagccga gcgtccgctc ccacgccggg ttgatgtcga
     3421 ggaagcagcc gcccgtgtcg agggtcgcca gcagggcctg cgagagttga aacagctgct
     3481 cgtacgtcga actcatctca ttgatccccg gggcgcccgg ccacgcccgg cagaacaatg
     3541 tctgccgggc ccctgcacgt caacgggtca gcgcgcggtc cctcggccga gggtcgttcg
     3601 cgcgccgccg ccgtgagccg acccgatctg tggctccggt cgccgacctg cgcgagcgag
     3661 cgaccggccg tgtgtccgcg tgaaccgtcg cgtcgctcgc agcggttgcg ccgggttcac
     3721 acgccgggat gcgtggccag ccacgcggtg gcgcgggcga ggttgggctg atgccccaga
     3781 cctcgccagt gctcctgggc cgcggtcgcg agggcgcggg cccgcggtcg gtcctgctcg
     3841 ctcggccaca gcgcctcggc gagcgccagc tggacgttcg cgtggtcctc gaccgagtgg
     3901 agcgtcaggg cccgctcgag cagcggcagg gccagcgccg gctcgccgcg ggccagcgcg
     3961 agttcgccga gcccgagcag cgacgcggcg acgttggcgt gcgagggccc gagcgcggcc
     4021 tcctgcaggg ccagcgcgcg ctcgtgcatg cgccgggcct cctcgtggcg gccgagctgt
     4081 cgcagcgagc ggccgaggtt gttgaggatc ggcgcgagat gaaatttgtc gacgccgagc
     4141 tcctcgcgca agctcagcgc gcgctcgagg atcgggatcg cctcgtcgtg ctcgccgagc
     4201 gccgcgagcg tgccacccag gccggcgagg atgccgttga tcgcagggtc ctcggcgccg
     4261 atcgcctgcc tgcgcagggc gagcgcgcgt tcgaaagtca cgcgcgcctc gtcgtgacgg
     4321 ccgagggcga gcagcgctcc gccgaggtgg ccgagcgcg
//
LOCUS       DASWNJ010000051         2800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_354232, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000051 DASWNJ010000000
VERSION     DASWNJ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354232"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2499)
                     /locus_tag="VGB85_00970"
     CDS             complement(<1..2499)
                     /locus_tag="VGB85_00970"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEY0132607.1"
                     /translation="MIFCNSRRLAERLAAAINETAGEELALAHHGSVARDRRQMIEER
                     LKRGELPAIVATSSLELGIDIGAVDLVIQIEAPPSVASGIQRIGRAGHQVGAVSRGIL
                     VPKFRGDLLACAAATPHMKAGLVEESHYPRSPLDIVAQQVVAALAMDPWEVDALYAMI
                     RGAAPFAELPRAAFEGVLDMLSGRYPSDEFAELRPRIVWDRVTGQLKSREGSKRLAVL
                     SGGTIPDRGLYGVFLTGEAGKPGRRVGELDEEMVFESRVGEVFVLGASSWRIEDITHD
                     RVVVSPAPGQPGKMPFWHGDNPGRPIAFGRVIGALSREIAKGSEAKVIARLHEEHGLD
                     EKAAANLRKYLREQAEATGEVPSDQTIVIERYLDELGDYRVCLLSPFGARVHAPWAMA
                     IAARIREESGAEVETMWSDDGIVLRFPASDGPPEVDILLIRPDEIEDRVVACLGSTAV
                     FAARFRENAARALLLPRRFPGKRSPLWAQRQRSADLLKVASRYGSFPMILETYRECLR
                     DVFDLPALTGLLRQIEARELRVHVVDTRTASPFAASLMFAYVANFIYEGDAPMAERRA
                     QALAVDAVQLKELLGEATLRELLDPDAITDTEQQLQRLGGHRPATSADGLHDLLLSLG
                     DLTLAEIEARCKPAESAQEWLRALERERRVFSYLPVAALLAAGAEGRTEASVPELRRH
                     AAAEDAGRLRDAIGVVPPVGLPRALMESVADAVGDLVSRYARTHGPFTLEAAAARIGL
                     GVAPVRAALVRLAAAERVLEGEFTPGGRGREWCDAGVLRSLKRRSLARLRREVEPVPP
                     AALGRFLPHWQGVMRPRAGLEALLSVVEQLQGAPL"
     gene            2486..>2800
                     /locus_tag="VGB85_00975"
     CDS             2486..>2800
                     /locus_tag="VGB85_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132608.1"
                     /translation="MQKIIVERCSRISATSRGWIDGQIDRRPPAAEPDARSASGAGFG
                     RPATTAPAGAASARRPLPPREGSLKNVPVPEDMSPDRAAPAPEDMSPDRAAPAPEVIS
                     PDR"
BASE COUNT          390 a         1149 c          919 g          342 t
ORIGIN      
        1 cagcggcgcg ccctgcaatt gctcgacgac cgacagcagc gcctcgagcc cggcccgcgg
       61 ccgcatcacc ccctgccagt gcggcaggaa ccgcccgagc gccgccggtg gcaccggctc
      121 gacctcccgg cgcaggcgcg cgaggctgcg acgcttgagc gatcgcagca cccccgcgtc
      181 gcaccactcg cgtccgcgac cgcccggcgt gaactccccc tcgagcacgc gctccgccgc
      241 cgccagccgc accagcgccg cgcgcaccgg cgcgaccccg aggccgatgc gtgccgcggc
      301 cgcctcgagc gtgaacggcc cgtgcgtgcg tgcgtagcga gacaccaggt ccccgaccgc
      361 gtccgcgacc gactccatca gcgcccgcgg aagccccact ggcggcacca cgccgatcgc
      421 gtcacgcagg cgcccggcgt cctcggccgc cgcgtgccgg cgcagctcgg gcacgctcgc
      481 ctcggtgcgc ccctcggccc ccgcggcgag cagcgccgcg accggcagat acgagaacac
      541 ccgccgctcg cgctcgagcg cccgcaacca ctcctgcgcc gactcggccg gcttgcagcg
      601 cgcctcgatc tccgcgagcg tcaggtcgcc gagcgacagc agcaggtcgt gcaggccgtc
      661 cgccgaggtc gccggccggt gcccgccgag gcgctgcagc tgctgctcgg tgtcggtgat
      721 cgcgtcgggg tcgagcagct cgcgcagcgt cgcctcgccg agcagctcct tgagctgcac
      781 cgcgtcgacc gcgagcgcct gggcccgccg ctcggccatc ggcgcgtcgc cctcataaat
      841 aaaattcgcg acgtacgcga acatcagcga cgccgcgaac ggcgacgccg tgcgcgtgtc
      901 gacgacgtgc acccgcagct cccgcgcttc gatctgccgc aacagacccg tcagcgccgg
      961 caggtcgaac acgtcgcgca ggcactcgcg gtacgtctcg aggatcatcg ggaacgaccc
     1021 gtagcgcgac gcgaccttca gcagatccgc cgaccgctgg cgctgggccc acagcggcga
     1081 gcgcttgccg gggaaccgcc gcggcagcag cagcgcccgc gcggcgttct cgcggaagcg
     1141 tgccgcgaac acggccgtgc tgccgaggca cgcgaccacg cgatcttcga tctcgtcggg
     1201 gcggatcagc aagatgtcga cctcgggcgg cccgtcggag gccgggaagc gcaggacgat
     1261 gccgtcgtcg gaccacatcg tctcgacctc cgcgcccgat tcctcccgga tccgcgccgc
     1321 gatcgccatc gcccacggcg cgtgcacgcg ggccccgaag ggcgacagca ggcacacgcg
     1381 atagtcgccg agctcgtcga ggtaacgctc gatcacgatc gtctgatcgc tcggcacctc
     1441 gccggtcgcc tccgcctgct cgcgcaggta ttttcgcaga ttcgccgcgg ccttctcgtc
     1501 gagcccgtgc tcctcgtgca gcctggcgat caccttcgcc tcgctgccct tggcgatctc
     1561 ccgcgacagc gccccgatca cgcgcccgaa cgcgatcggc cgcccggggt tgtcaccgtg
     1621 ccaaaacggc atcttgcccg gctgccccgg cgccggcgac acgaccacgc ggtcgtgcgt
     1681 gatgtcctcg atccgccacg acgacgcgcc gagcacgaac acctcgccga cccgcgactc
     1741 gaacaccatc tcctcgtcga gctcgccgac ccgccgaccc ggcttgcccg cctcgccggt
     1801 caggaacacc ccgtagagcc cgcggtcggg gatcgtgccg ccgctgagca ccgccagccg
     1861 cttcgatccc tcgcgcgact tgagctgccc ggtcacccgg tcccacacga tccgcggccg
     1921 cagctccgcg aactcgtccg aggggtagcg ccccgagagc atgtcgagca cgccctcgaa
     1981 ggcggcccgc ggcagctcgg cgaacggcgc cgcgccccgg atcatcgcgt acagcgcgtc
     2041 gacctcccac gggtccatcg ccagcgccgc gaccacctgc tgcgccacga tgtcgagcgg
     2101 gctgcgcgga tagtggctct cctcgacgag ccccgccttc atgtgcggcg tcgcggccgc
     2161 gcaggcgagc aggtcgccgc gaaacttcgg caccaggatc ccgcgcgaca cggcgccgac
     2221 ctggtgcccc gcgcgaccga tccgctggat ccccgaggcg accgacggcg gggcctcgat
     2281 ctggatcacg aggtcgaccg cgccgatgtc gatgccgagc tcgagcgagc tggtcgccac
     2341 gatcgccggg agttctcctc gcttgagccg ctcctcgatc atctgccgac ggtcgcgcgc
     2401 gaccgacccg tggtgcgcga gcgccagctc ctccccggcc gtctcgttga tcgccgccgc
     2461 cagccgctcg gccaggcgcc ggctgttgca gaagatcatc gtcgagcgat gctcgcggat
     2521 cagcgcgacc agccgcgggt ggatcgacgg ccagatcgac cgccgcccgc ccgccgccga
     2581 gcccgacgcc aggtccgcca gcggggccgg ctttggcagg cccgcgacga ccgccccggc
     2641 cggcgccgcc tcggcccgcc gccccctgcc cccgcgcgaa gggtctttaa aaaatgtgcc
     2701 tgtcccggag gacatgtcgc cggaccgcgc cgcgcctgca ccggaggaca tgtcaccgga
     2761 ccgcgccgcg cctgccccgg aggtcatatc gccggaccgc
//
LOCUS       DASWNJ010000052         2532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_495224, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000052 DASWNJ010000000
VERSION     DASWNJ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495224"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VGB85_00980"
     CDS             <1..677
                     /locus_tag="VGB85_00980"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0132609.1"
                     /translation="PAAFEQCDDGLESATCDADCTLAECGDGQLSPLAGEACDDGNLS
                     DNDDCSSSCQKLRRTIFVTSKLYTGKLGGLAGADAQCQMLADNAKLPGVFLAWLSDGA
                     VTPASRFVHSDVPYVLTTGIPVAQNWKDLTDDTLIHAVDTTESQGAAPIPVGGCGGGT
                     KPTVWTNSLANGNAWGVDACGGWNSTTGIGRLGHAKATSFAWSKFCEGQAGSCAWQAA
                     LYCVEQ"
     gene            complement(739..1800)
                     /locus_tag="VGB85_00985"
     CDS             complement(739..1800)
                     /locus_tag="VGB85_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY0132610.1"
                     /translation="MIKLRPWKGTKNEFEADIIVNGANGRALRKRVKAPVTGKSSAER
                     WARAIEQEMLTQLLAPEPEPEKPPAPTFEEFAVIFLDLCRANRLGVNTLSHYEVHLRL
                     YLNPVIGKRRLDAVKPADITAIKASLTGKAQNTMSETIKTLRRVYNVAIEQGVIEHLP
                     VKIDVPRRSRKAVVAYDAVQQAALLRAAKELGQRHVVMLLLGMDAGLRRGEMLALKWT
                     DLDFTQGCMVVRHNIVRGKLDRPKGRTEDEIALTPRLAEALQAYRHAGPFVFTNDRGE
                     HFIETNIASWMKKLVALAGVPWHGTHILRKTCGTRIADGGGGVGAVAGHLRHKDLQTA
                     SRYIDRRGASTRALVALES"
     gene            complement(1797..2105)
                     /locus_tag="VGB85_00990"
     CDS             complement(1797..2105)
                     /locus_tag="VGB85_00990"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0132611.1"
                     /translation="MKAPGPVVAAGQSHGPQDRARAIPGRTGHEGVRSRREVELPPPL
                     LTVRDLAVLLATSSSGVYSRVARGQIPGVLRIGRSLRFDPRVIAQWLSANKASAGGLS
                     "
     gene            complement(2261..>2532)
                     /locus_tag="VGB85_00995"
     CDS             complement(2261..>2532)
                     /locus_tag="VGB85_00995"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEY0132612.1"
                     /translation="SNGKIERYHRTLDKGTIHALTSDGAHATIAARIDHYNNHRLHSA
                     IGYVTPRDKLLGRETEIWTERDRKLEQARELRRKRRELDHLGAAA"
BASE COUNT          384 a          831 c          882 g          435 t
ORIGIN      
        1 acccggcggc cttcgagcag tgcgacgacg gcctcgagag cgcgacctgc gacgccgact
       61 gcacgctcgc cgaatgcggt gacggccagc tgagcccgct cgcgggcgag gcgtgcgacg
      121 acggcaacct cagcgacaat gacgactgct cgagcagctg tcagaagctg cgccggacga
      181 tcttcgtcac cagcaagctg tacaccggca agctcggcgg tctggccggc gccgacgcgc
      241 agtgccagat gctcgccgac aacgccaagc tcccgggcgt gttcctggcc tggctcagcg
      301 acggcgcggt cacccccgcg agccgcttcg tgcactccga cgtcccctac gtgctgacca
      361 ccgggatccc ggtcgcgcag aactggaagg acctgaccga cgacacgctg atccacgcgg
      421 tcgacaccac cgagagtcag ggcgccgcgc cgatccccgt cggcggctgc ggcggcggga
      481 ccaagccgac cgtgtggacc aacagcctcg ccaacggcaa cgcgtggggc gtcgacgcct
      541 gcggcggctg gaacagcacc accggcatcg gccgcctcgg ccacgccaag gcgaccagct
      601 tcgcgtggtc caagttctgc gagggccagg cgggcagctg cgcctggcag gccgcgctgt
      661 actgcgtcga gcagtgatcg tagggctaat tgatcctgta gttacaaggc cttggaggcg
      721 ggcggtagca cgcctccatc agctctccag cgccacgagc gcgcgggtgc tggcgccgcg
      781 acggtcgatg tagcggctcg cggtctgcag gtccttgtgc cggaggtgcc cggcgacggc
      841 cccgacacca ccaccgccgt cggcgatgcg cgtcccgcag gtcttgcgaa ggatgtgcgt
      901 gccgtgccac ggcacacccg ccagagcgac gagctttttc atccacgacg cgatgttcgt
      961 ctcgatgaag tgctcgccgc gatcgttcgt gaacacgaac gggccggcat gtcggtacgc
     1021 ttgcagagcc tcggcgagcc gcggcgtcag ggcgatctca tcctcggtgc ggcccttcgg
     1081 gcgatcgagc ttcccacgca cgatgttgtg acgcacgacc atgcaaccct gcgtgaagtc
     1141 gaggtccgtc cacttgagcg cgagcatctc accgcgccgc aggccggcgt ccatgccgag
     1201 caggagcatc accacgtggc gttgcccgag ctccttcgcg gcgcggagca gggctgcctg
     1261 ctggaccgcg tcgtaggcaa ccacggcctt gcgagatcgc ctgggcacgt cgatcttgac
     1321 gggcaggtgc tcgatcacac cctgctcgat ggcgacgttg tagacccgcc gcagggtctt
     1381 gatcgtctcg gacatcgtgt tctgggcctt gccggtgagg ctcgccttga tcgcggtgat
     1441 gtccgcgggc ttgaccgcgt ccaggcgccg cttcccgatc accggattca ggtacagccg
     1501 cagatgcacc tcgtagtgcg agagcgtgtt cacgccgagg cggttggccc tgcacaggtc
     1561 gaggaagatc accgcgaact cctcaaaggt gggcgcgggc ggcttctcgg gctccggctc
     1621 gggggcgagg agttgcgtga gcatctcctg ctcgatcgcg cgggcccacc gctccgcgct
     1681 ggacttgccc gtgaccggtg ccttgacgcg cttgcgcaag gcccggccgt tggctccgtt
     1741 gacgatgatg tcggcttcaa attcattctt cgttcccttc cacgggcgca gcttgatcat
     1801 gagagacctc ctgcgctcgc cttgttcgcc gagagccatt gtgcgatgac ccgcgggtcg
     1861 aagcgaaggc tgcgaccgat gcgaagaaca ccggggatct ggccgcgtgc aacccgggag
     1921 taaacgccgc tactgctcgt ggcgagcaac acggcgaggt ctcggaccgt gagcagcggc
     1981 ggcggcagct cgacctctct gcgcgatctc acgccttcat gaccggtgcg acctgggatc
     2041 gcacgggccc tgtcttgtgg gccatggctc tgccccgcgg ccaccacagg accaggcgcc
     2101 ttcacggtct tctcccttcc actcgttcct cagtccgtgc cgacctgacg ccggcattta
     2161 gcgcgacggc aggcggtact gtattggctc agcgtgaacg agacagatgg gtgggttgac
     2221 gaacggacgg gttaagggcg aggtgaaggg ttgagggaag ttatgcggcg gcgccgaggt
     2281 ggtccagttc tcggcgcttg cgacggagct cgcgggcttg ctcgagcttg cgatcgcgtt
     2341 cggtccagat ctcggtctcg cggccgagga gcttgtcacg cggggtgacg tagccgatgg
     2401 cgctgtgcag gcggtggttg ttgtagtggt cgatgcgggc ggcgatggtg gcgtgggcgc
     2461 cgtcggacgt gagggcgtgg atggtgccct tgtcgagggt tcggtggtag cgctcgatct
     2521 tgccgttgga ct
//
LOCUS       DASWNJ010000053         2253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_318786, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000053 DASWNJ010000000
VERSION     DASWNJ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318786"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VGB85_01000"
     CDS             complement(<1..268)
                     /locus_tag="VGB85_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO family protein"
                     /protein_id="HEY0132613.1"
                     /translation="MPMNFAFDTTYARLPDRFFSKVAPTRVRDPRVVKVNRSVAALLG
                     LDADLLASPEGAQILAGNVVPEGAASIALAYAGHQFGHFAGQLGD"
     gene            558..821
                     /locus_tag="VGB85_01005"
     CDS             558..821
                     /locus_tag="VGB85_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132614.1"
                     /translation="MNRHARKIPLGAAALSLLLALPGCGKKNDADTVSPGAERGGAGA
                     DGNNSTPDSLTNGEAAPDPDTTSVTEPTDAGGSAVNGATDTPH"
     gene            859..1470
                     /locus_tag="VGB85_01010"
     CDS             859..1470
                     /locus_tag="VGB85_01010"
                     /inference="COORDINATES: protein motif:HMM:NF016843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HEY0132615.1"
                     /translation="MTEERDEHQPGGVPGRLARLRLAPLLRRFPQRPVWAAFVLINGF
                     VTMALLAGLAMLTRTPFIFPSLGPTAFLLFFSPTAASASPRNTLLGHAIGIVCGYGAL
                     LLLGLEHAAPAIMTGVDDPRIFAAALSLALTGAIMILLDAAHPPAGATTLIISLGIMT
                     HPVDLLVIEVAVALLIVQAFVINRLAGVDYPVWRAARSSASRE"
     gene            1827..>2253
                     /locus_tag="VGB85_01015"
     CDS             1827..>2253
                     /locus_tag="VGB85_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132616.1"
                     /translation="MNRPGSSWIFCIGVLLGAAGLPGCDDPEDTQWRANIDPCGCVID
                     AAGAVDCDAVPTDLLDECRAGGDGRCDPRDSTDPDCVPDGRCDLADRRDPDCYPDGYC
                     DPRNHIEPDCREDLCDPTHVQPDGSCGDGVCDTADSTDPD"
BASE COUNT          336 a          787 c          800 g          330 t
ORIGIN      
        1 cgtcgccgag ctggccggcg aagtggccga actgatgccc ggcgtaggcg agcgcgatcg
       61 aggcggcgcc ctccggcacg acgttgccgg cgaggatctg cgcgccctcg ggcgaggcca
      121 gcaggtcggc gtcgaggccg agcagcgccg cgacggagcg gttgaccttg accacccgcg
      181 ggtcgcggac gcgcgtcggc gcgaccttcg agaagaagcg atcggggagg cgggcgtagg
      241 tggtgtcgaa ggcgaagttc atgggcatgc cggacgcatc gcgtgcgccg tggacccctc
      301 atcatgactg ctcgcggcga gcgtgccatc gtcacgcgag accaccgatg tgacgcagga
      361 ggccgacgag cgcaggaggc cgccaattcc cgtggaacga cgcgatgcgc acgctgcgcg
      421 accacagcgg atggactgca tggcgcgctc gccgacgctg cgcggcggcg gcgacggcca
      481 gcggcgccgg gcccgtccgc gggcgcttgc gcgggtcgcc tcgcgtcgca tagcgatcgg
      541 acctcacgga gtccgccatg aaccgtcacg ctcgcaagat cccgctcggt gccgccgccc
      601 tgtcgctcct cctcgccttg cccggttgcg gcaagaagaa cgacgccgat accgtgtcgc
      661 cgggcgccga gcgtggtggt gccggcgcgg acggcaacaa cagcaccccc gacagcctca
      721 ccaacggcga ggcggccccg gatcccgaca cgacctcggt caccgaaccg accgacgcgg
      781 gcggctcggc cgtcaacggc gcgaccgaca cgccgcactg atcttcacat gtcgtgatgc
      841 tctgttacgc tcgcgtcgat gaccgaggag cgtgacgagc accagcccgg aggtgtgccc
      901 ggccgcctgg cgcggctgcg cctcgcgccg ctgctgcgcc gcttcccgca gcggccggtg
      961 tgggcggcgt tcgtgctgat caacggcttt gtcaccatgg cgctgctcgc cggcctggcg
     1021 atgctgacgc gcacgccctt catcttccct tcgctcggac cgaccgcgtt cctgctgttc
     1081 ttcagcccga cggcggcctc cgcgagcccg cgaaataccc tgctcggcca cgcgatcggg
     1141 atcgtctgtg gatatggtgc gctgctgtta ttgggcctcg agcacgcggc cccggcgatc
     1201 atgaccggcg tcgacgaccc gcggatcttc gccgcggcgc tgtcgctcgc gctgaccggc
     1261 gcgatcatga tcctgctcga cgcagcccac ccgccggccg gcgcgacgac gctgatcatt
     1321 tcgctcggga tcatgaccca cccggtcgac ctgctcgtca tcgaggtcgc ggtcgcgttg
     1381 ttgatcgtgc aggcgttcgt catcaatcgc ctcgcgggcg tcgattaccc ggtgtggcgg
     1441 gccgcgaggt ccagcgcgtc acgcgagtga ggtcagctcg cgaaccgtgc atcgcgcgtc
     1501 agctgcgtgg gggagctcgc ggtcgacggg gggctcgggc aggggctaaa cccgccgcac
     1561 gacggacggt gatccctcgc acagcggacg cgtcggcgag atgtgtgctt cgcggtcgcg
     1621 gcggcgcatg gatcttgatc aatgaccgca atgtgacagt gatgaatgcg acgcgggcga
     1681 cccgcgcgcc tcacggatcc gaaatacatc gcgtcgacaa tggcaggatc ggcgaccggg
     1741 agggcgctgc cggccgcgag cgcgggcgcg gacggctttg gaaagactca cggcggcgcc
     1801 atcgaatcgg catggatgca agcaagatga atcgccccgg gagctcgtgg atcttttgta
     1861 tcggtgtcct gctcggcgcg gccggcctgc ctggctgcga cgatccggaa gacacgcagt
     1921 ggcgggccaa catcgatccc tgcggctgtg tcatcgacgc cgctggcgct gtcgattgcg
     1981 acgccgtgcc gaccgacctg ctcgacgagt gcagggcagg gggcgacggc cgctgcgacc
     2041 cgcgcgacag caccgacccc gactgcgtgc cggacggccg ctgcgacctc gcggaccgca
     2101 gggacccgga ttgttatccc gatggttact gcgatccccg caaccacatc gagcccgact
     2161 gccgcgagga tctgtgcgat ccgacgcacg tgcagcccga cggatcgtgc ggcgacggcg
     2221 tgtgcgacac ggcggacagc accgaccccg act
//
LOCUS       DASWNJ010000054         4327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_177553, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000054 DASWNJ010000000
VERSION     DASWNJ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177553"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..890
                     /gene="nth"
                     /locus_tag="VGB85_01020"
     CDS             228..890
                     /gene="nth"
                     /locus_tag="VGB85_01020"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEY0132617.1"
                     /translation="MKRAEKAEKIHQTLDRLYPRPVVPLGHHDPYTLLVAVVLSAQTT
                     DARVNMVTPALFERASTPAQMAALTVPEIRAYIRTCGLSPGQAANIHRLSQILVEQHG
                     GEVPRSFEALEALPGVGHKTASVVMCEAFGVPAFPVDTHIHRLAYRWGLSDGSKVERT
                     EHDLKAVWDRSLWPRLHLQMIYFGREYCPALRHAWEQCPICSWAGVKSRLLAERKKPT
                     AA"
     gene            1038..1637
                     /locus_tag="VGB85_01025"
     CDS             1038..1637
                     /locus_tag="VGB85_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEY0132618.1"
                     /translation="MRQQSLFPTSPAARADPVPGLTVQRDYIDEAEERRLLAHIDAGA
                     WDTDFRRKIQLYGLGYGGEGETAWVRDFPAWLDALARRIVADGWMPRMPDNCVINDYA
                     PGVGIGPHSDFAAFDAPLVALSLLSPVVINFRRRGHEDAAAIVVPPRSLWVAAGAARW
                     QWTHAIAARRSDVIDGERVRRGRRISLTFRVAKVLVVRP"
     gene            complement(1640..2386)
                     /gene="map"
                     /locus_tag="VGB85_01030"
     CDS             complement(1640..2386)
                     /gene="map"
                     /locus_tag="VGB85_01030"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY0132619.1"
                     /translation="MSVTIYGPRDIAAIRAASRAAAATLAWVGARLAPGVTTGDIDAW
                     VREDTARRGGRPSQLGYHGFPASVCTSRNEVVCHGVPRADEVLRAGDIVNVDVTTELD
                     GYHGDTSATFCIGEPPAAARRLVDTTRAALLAGIAAVRPGGRIGDIGAAIMEVAEAHG
                     LGVVREFCGHGIGRSMHEAPQVPHAGKAGTGLRLRAGMVFTIEPMLTLGAPTTRVLAD
                     GWTAVTTDGQWSAQFEHTICVTADGAEVLT"
     gene            complement(2476..3648)
                     /locus_tag="VGB85_01035"
     CDS             complement(2476..3648)
                     /locus_tag="VGB85_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY0132620.1"
                     /translation="MKVRERNENGRTVWQVDIHCIPANEAKPARFRLTPPDQVTTRSG
                     AERWGKDQWAKIIKDGRPYNTKQAREQRRAQEEEAQKLNVPTLTAFWPRFLEHIEAER
                     MAVNTIMAYEKAGRMHVLPQLGDRPIDQLAELDIQRLKASMKKLKPYTVNQALNALGG
                     ALKLAKLHHPHIVIPPIKRVRVSAEQHFRVYTPEEACALVEAATCPTRRVAILLGLVI
                     GLRRREVPALRWSDVDEVRGVITVRHQLLGWELVPPKHGKVRKVPMTASLREALKALP
                     RDTEWVLPRGKTGRSCVSLRATLKTVARHAGVPDHGPHALRHSYATHSLASGVDIRTV
                     SALMGHSNVVITARYLHLLPGAEQAAAGKLKDFYKKTTGATVTDLSRARAARTKKT"
     gene            complement(3731..4060)
                     /locus_tag="VGB85_01040"
     CDS             complement(3731..4060)
                     /locus_tag="VGB85_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132621.1"
                     /translation="MDDREAAGLELRAVRDAAIKAGLEELGDAFQAAIKPTKTSRRKA
                     PLPPAPADAPLMTRAEVACLLRTTARQISNMTARGQLPAPVRVTGLGLRWPRAAVAAW
                     IAEQMAA"
     gene            complement(4060..>4327)
                     /locus_tag="VGB85_01045"
     CDS             complement(4060..>4327)
                     /locus_tag="VGB85_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132622.1"
                     /translation="KQFGVSKRAAFNAAMRLSYAWVADTTAEQERRRRAVEGVRDEAR
                     RERTAELDQLTIVALERAAPPQNQVRRRAPHTPPTRAPRSKGTK"
BASE COUNT          595 a         1485 c         1531 g          716 t
ORIGIN      
        1 gccgcgaaga ccagcgctgc gaggcagggg gacttcagca tgggccgcac tatacccgag
       61 tgtgccgccc ccgcgcgcgt cgtcgatcac gaacgagcga cgcgcgctca gtgccgtcgc
      121 acgcggcggc aagcggggaa aaaccccgtg cgggcgtgcc cacgacggac gtggcagccg
      181 tcggtcgatt tcgcgccagg atggcgtacg ctggtctgcg aagcccgatg aagcgcgccg
      241 agaaagccga gaagatccat cagaccctcg accgcctgta cccgaggccg gtggtcccgc
      301 tggggcacca cgacccctac acattgctgg tggccgtcgt gctgtcggcg cagacgaccg
      361 acgcgcgcgt caacatggtg acgcccgcgc tgttcgagcg ggcctcgacg ccggcgcaga
      421 tggccgcgct gacggtcccg gagatccgcg cgtacatccg gacctgcggg ctgtcgccgg
      481 gccaggcggc caacatccat cgcctctcgc agatcctcgt cgagcagcac ggcggcgagg
      541 tgccgcgctc gttcgaggcc ctcgaggcgc tgcccggcgt cggccacaag accgcgagcg
      601 tcgtcatgtg cgaggccttc ggcgtgccgg ccttcccggt cgacacgcac atccatcgcc
      661 tcgcatatcg ctggggcctg tcggacggca gcaaggtcga gcgcaccgag cacgacctca
      721 aggcggtgtg ggaccgctcg ctgtggccgc ggctgcattt acagatgatc tacttcggtc
      781 gcgagtattg cccggcgctg cgccacgcgt gggagcagtg cccgatctgc tcgtgggccg
      841 gcgtgaagtc gcggctgctg gccgagcgca agaagccgac ggcggcgtag atcagcccgg
      901 cttgcgagcg aacaggcgtg accaccacga gcgctgcgcg gcgggcccgg gcggcggtgg
      961 agctgccggc gcgggctggc cgggcgcggg gcgctcccgc caggctccgg tccgggaggg
     1021 tctgctaacc tggggccgtg cgccagcaat cgctgttccc gacgagcccc gcggcgcggg
     1081 cggacccggt gccggggttg acggtgcagc gggattacat cgacgaggcc gaggagcggc
     1141 gcctgctcgc gcatatcgac gcgggcgcct gggacaccga cttccggcgc aagatccagc
     1201 tgtatgggct cggctatggc ggcgagggcg agaccgcgtg ggtgcgggac ttcccggcgt
     1261 ggctcgacgc gctcgcgcgc cggatcgtcg ccgacgggtg gatgccgcgg atgcccgaca
     1321 attgcgtgat caacgactac gccccgggcg tcgggatcgg gccgcacagc gacttcgcgg
     1381 ccttcgacgc gccgctggtc gcgctgtcgc tgctctcgcc ggtggtgatc aactttcgtc
     1441 gccgcggcca cgaggacgcc gcggcgatcg tcgtgccgcc gcggagcctg tgggtcgccg
     1501 ccggcgcggc gcgctggcaa tggacgcacg cgatcgcggc gcgtcgctcg gatgtcatcg
     1561 acggcgagcg cgtccgtcgc ggccgccgga tctcgctgac ctttcgtgtc gcgaaagtgc
     1621 tggtcgtgcg gccatgattt tatgtcagta cttcggcgcc gtcggcggtc acgcagatcg
     1681 tgtgctcgaa ctgcgcgctc cactggccat cggtggtgac ggcggtccag ccatcggcga
     1741 gcacgcgggt cgtcggcgcg ccgagggtca gcatcggctc gatcgtgaac accatccctg
     1801 cgcgcaggcg caggccggtg ccggccttgc ccgcgtgcgg cacctggggc gcctcgtgca
     1861 tgctgcggcc gatcccgtgg ccgcagaact cgcggacgac cccgagcccg tgcgcctccg
     1921 cgacctccat gatcgccgcg ccgatgtccc cgatccgtcc gccgggccgc accgccgcga
     1981 tgccggcgag cagcgcggcc cgggtcgtgt cgaccaggcg gcgcgccgcg gccgggggct
     2041 cgccgatgca gaacgtcgcc gaggtgtcgc cgtgatagcc gtcgagctcg gtcgtcacgt
     2101 cgacgttgac gatgtccccg gcgcgcagca cctcgtcggc gcgcgggacc ccgtggcaga
     2161 cgacctcgtt gcggctggta cacacgctcg cggggaagcc gtgatagccg agctggctcg
     2221 gccgcccgcc gcgacgcgcg gtgtcctcgc gcacccacgc gtcgatgtcg cccgtcgtca
     2281 ctcccggggc cagccgggcc cccacccacg ccagcgtcgc cgcggcagcg cggctcgccg
     2341 cgcggatcgc cgcgatgtcg cgcggcccat agatcgtcac actcatgccg cgagggtgcc
     2401 gtggtcaacg ccctgcgatc caataccgtt ttggtatgcg accgggtttg ctaagtagtt
     2461 gaaaacaagg ggttatcagg tcttcttggt gcgcgctgcc ctggcccgtg acaggtccgt
     2521 gacagttgcg cccgtcgtct tcttgtagaa gtccttgagc ttcccggcgg ccgcctgctc
     2581 cgcgccgggg agcaggtgca ggtagcgcgc ggtgatgacc acgttcgagt gccccatcag
     2641 ggccgagacg gtcctgatgt cgacccccga tgccagggag tgggtcgcgt aggagtgccg
     2701 gagcgcgtgg ggtccgtgat caggcacgcc tgcatgacga gcgacggtct tcagggttgc
     2761 tcggaggctc acgcaggagc ggccggtctt ccctcggggc agcacccact cggtatccct
     2821 gggcagggcc ttgagtgctt cacgcaggct cgcggtcatg gggaccttcc gaaccttgcc
     2881 gtgcttgggt ggcaccagtt cccaacccaa gagctggtga cggacggtga tcacgccgcg
     2941 gacctcgtcc acgtcggacc atcgtagggc agggacctcg cgtcggcgca ggccgatcac
     3001 gaggcccagg aggatggcca ctcgtctggt tggacacgtg gcggcttcca cgagggcaca
     3061 tgcctcttcg ggcgtgtaga cgcggaagtg ttgctcggcg gacacccgca ccctcttgat
     3121 cggcgggatg acgatgtgcg ggtgatggag cttggcgagc ttcaacgcac ccccgagcgc
     3181 attgagtgcc tggttcaccg tatagggctt gagcttcttc atcgaagcct tcagcctctg
     3241 gatgtcgagt tctgcgagct gatcgatcgg acggtccccg agctggggga gcacgtgcat
     3301 gcgtcccgcc ttctcgtagg ccatgatcgt gttgacggcc atccgctccg cctcgatgtg
     3361 ttcgaggaac cggggccaaa aggccgtcag agtagggacg ttgagctttt gtgcttcctc
     3421 ctcttgcgcg cgacgctgtt cacgcgcctg cttggtgttg taggggcggc catccttgat
     3481 gatcttcgcc cactggtcct tgccccaccg ttcggcacct gaccgggtgg tgacctggtc
     3541 aggtggcgtc aggcggaagc gagccggctt cgcttcattg gctgggatgc agtggatgtc
     3601 gacctgccac acggttcgcc cgttctcgtt ccgttctctg accttcatgg gttctttccc
     3661 ccacgcaggc ccgcactacg acccttagat cggatgtacg ggcgagcgca agcggtactt
     3721 cgggaacgag ctacgcggcc atctgctcag cgatccaagc cgccaccgcg gcccgaggcc
     3781 accgcagccc gagacccgtc acgcgcaccg gagcagggag ctggcctcga gcggtcatat
     3841 tcgagatctg ccgcgccgtc gtgcggagta ggcacgcgac ctcagcccgc gtcatcagcg
     3901 gcgcgtcggc gggtgcgggc ggcagcggtg ccttgcgacg cgaggtcttc gtcggcttga
     3961 tggcggcctg gaacgcgtcg ccgagttctt cgagacctgc cttgatcgca gcatcccgca
     4021 cggcacgcag ctcgagcccc gctgcttcgc ggtcgtccat cacttcgtcc ccttgctccg
     4081 cggcgcgcgg gtcggcgggg tgtgaggcgc ccggcgtcgg acttgattct gaggaggggc
     4141 cgcgcgctcg agcgcgacaa tcgtgagctg gtccagctca gcagtccgct cgcggcgcgc
     4201 ctcatcgcgg acgccctcga cagcgcgacg acgcctctcc tgttcggccg tggtgtcagc
     4261 gacccacgcg tagctcagcc tcatcgccgc attgaaggcc gcccgcttgc tgacgccgaa
     4321 ctgcttt
//
LOCUS       DASWNJ010000055         3520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_354305, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000055 DASWNJ010000000
VERSION     DASWNJ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354305"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VGB85_01050"
     CDS             complement(<1..266)
                     /locus_tag="VGB85_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132623.1"
                     /translation="MTGDPLRLLDDPTIGATLRTDLGAASHAAAYDVEAGLARFETTL
                     ATNMARGTGAVGGGMSGGFAVGALVLAGGLAAALGWQLAGPAPAV"
     gene            complement(263..1102)
                     /locus_tag="VGB85_01055"
     CDS             complement(263..1102)
                     /locus_tag="VGB85_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0132624.1"
                     /translation="MKGLADPRHTPPEDGNPPAQPPHLDAARLFRDHAQFVASFVVRL
                     GMRREEVDDIVQEVFLVAHRRGGFIPGAARPTTWLAEIALRVSSSARRRQRRSREDPD
                     VQTIAGSMSPARGPGEQAEAAEALRRVQQTLEALDEAKRAVFILFELEGESCESIAAG
                     LGIPVGTVYSRLHKARKLFMEVHARLLGGPKLPGPASDPPPHGPSGLPPHGPSGLPPH
                     GPSGLPPHGPSGLPPHGPSGLPPHGPAGLQRSGPTGLDLPGSESRDLPGPGRKRNSHM
                     VTQ"
     gene            complement(1099..2244)
                     /locus_tag="VGB85_01060"
     CDS             complement(1099..2244)
                     /locus_tag="VGB85_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132625.1"
                     /translation="MTLRASSAAGATGRIASGARARGIRVVVAPVVLAALAGLPMRAA
                     AAPGLWVRSAPVEGPAQLPAPQQPPQPQQPIIAALSLSAGATCLSADTLAEHVTTWLG
                     RDKIAVSVAIEVRGDTFARNAAVIVVRTPAGLVERPFDEGPTGCSDFHAVLGLAIAMA
                     IDSSVLVGLGYEVIDTAPPPVSAPAVDNERPPLLARRPASAPAPRRARLRALAAVRGG
                     LWVGVVPGVAGGGQLQVELVWRSWFELRLGVLGGYGGRRALGAGTVDLGLVAGRVDAC
                     FGVQRRRLRPRLCIGPAAGVLQARARGYEAATDIVSPWLAAAAALELRVVTTRVFAVD
                     FVIDAVVPVIKPVVLVRAPDKPGMIGDAVSFAPVGMVIGVGGAFTIR"
     gene            2389..3246
                     /locus_tag="VGB85_01065"
     CDS             2389..3246
                     /locus_tag="VGB85_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132626.1"
                     /translation="MRLALLAVSCLVVACGGGEPVQLAPQAEKLTIEAPKSRGAQRYV
                     IAATGGKLEFDMEAPIEKIRGRVPEAAVSGEIFVDASDLTKTTGLVHVDLRELELFQR
                     TAPAEGQPFGEETRSDKQNEHARAWLEIGADTPEDVLKKNTLVEFSLREVKDASATDL
                     SKLTGPERKVTFTATGEFLLHQRKASKSAKLEATFTWAGDAPTGITIKTLEPLEIGLE
                     EYDVRPREGFSKLAAKTLQALSPKVASAAQVNFEFTAKLAPDAKPTMQSVAAEATPTT
                     PPAPAAPAG"
     gene            3298..>3520
                     /locus_tag="VGB85_01070"
     CDS             3298..>3520
                     /locus_tag="VGB85_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132627.1"
                     /translation="MDIRTITATLAILGTGLTLTACKKSSSDASEVPASQQPAGEGSC
                     GPDKGHGHANCSGDKTDAPASEPTGDDAAA"
BASE COUNT          527 a         1334 c         1253 g          406 t
ORIGIN      
        1 acggccggcg ccggaccggc gagctgccag ccgagcgccg ccgcgaggcc gcccgcgagc
       61 accagcgcgc cgacggcgaa gccgcccgac atcccgccgc cgaccgcacc tgtcccccgg
      121 gccatgttgg tcgcgagcgt cgtttcgaag cgcgcgaggc cggcctcgac gtcgtatgcg
      181 gcggcgtggc tggccgcgcc gaggtcggtc cggagtgtcg cgccgatcgt cgggtcgtcg
      241 agcagcctga ggggatcacc agtcattgcg tcaccatgtg agagtttcgc ttccggcccg
      301 ggcccggaag gtcccgactc tccgagccgg gaaggtcgag gcccgtgggg ccggatcgtt
      361 gcaggcccgc agggccgtga ggtggcaggc cggaagggcc gtgaggtggc aggccggaag
      421 ggccgtgagg tggcaggccg gaagggccgt gaggtggcag gcccgaaggg ccgtgaggtg
      481 gcaggcccga agggccgtga ggtggcgggt cactcgcggg gcccggcagc ttcggtccgc
      541 cgagcaggcg cgcgtggacc tccatgaaca gcttgcgggc cttgtgcagg cgcgagtaca
      601 cggtgccgac cgggatgccg aggccggccg cgatcgactc gcagctctcg ccctcgagct
      661 cgaacaggat gaacaccgcg cgtttggcct cgtcgagggc ctcgagggtc tgctggaccc
      721 ggcgcagggc ctcggcggcc tcggcctgct cgccggggcc gcgcgcgggc gacatgctgc
      781 cggcgatcgt ctggacgtcc gggtcctcgc gcgagcgtcg ctggcgtcgt cgcgcggacg
      841 agctgacgcg cagggcaatc tcggcgagcc aggtagtggg gcgcgccgcc cccgggatga
      901 agccgccccg gcggtgcgcg acgaggaaca cctcctgcac gatgtcgtcg acctcttcgc
      961 ggcgcatccc gaggcgcacg acgaagctgg cgacgaactg cgcgtggtcg cgaaacaacc
     1021 gggccgcgtc gaggtgcggg ggctgtgccg gcgggttgcc atcttcaggc ggtgtatgtc
     1081 gcggatcggc caagcccttc accggatcgt gaaagcaccg ccgactccga tgaccatccc
     1141 gacgggggcg aagctcacgg cgtccccgat catacccggt ttgtccgggg cccggaccag
     1201 gaccacgggt ttaatgaccg gcacgacggc gtcgatcacg aagtcgaccg cgaacacccg
     1261 cgtcgtcacc acccgtagct ccagcgcagc cgccgcggcg agccaggggc tgacgatgtc
     1321 ggtcgccgcc tcgtagccgc gggccctcgc ctgcaacacg cccgccgcgg ggccgatgca
     1381 cagccgcggt cgcaggcgcc tgcgctggac cccgaagcag gcgtccacgc gtccggccac
     1441 cagtccgagg tcgacggtcc cggcgccgag cgcccgccga ccgccataac caccgagcac
     1501 gccgaggcgc agctcgaacc acgaccgcca gacgagctcg acctgcaact gcccgccccc
     1561 cgcgacgccc ggcaccacgc cgacccacag cccgccgcgc acggccgcca gcgcgcgcag
     1621 ccgcgctcgc cggggcgcgg gtgcgctcgc cggccggcgc gccagcagcg gcggacgctc
     1681 gttgtcgacc gccggcgcgc tcaccggcgg cggcgccgtg tcgatcacct cgtagccgag
     1741 cccgacgagc accgacgagt cgatcgccat cgcgatcgcc agaccgagca ccgcgtggaa
     1801 gtcgctgcac ccggtcgggc cctcgtcgaa cggccgctcg accagccccg ccggcgtgcg
     1861 cacgacgatg accgccgcgt tgcgggcgaa cgtgtcgccg cggacctcga tcgccacgct
     1921 gaccgcgatc ttgtcgcgcc cgagccaggt cgtcacgtgc tccgcgagcg tgtcggcgga
     1981 cagacaggtc gcgccggcgc tcagcgacag cgcggcgatg atcggttgtt gcggctgtgg
     2041 cggctgctgt ggtgctggca actgcgccgg accctcgacc ggcgccgacc tgacccaaag
     2101 gccaggtgcc gccgcggcgc gcatgggcag ccccgcgagc gcggcgagca cgaccggcgc
     2161 gactacgacg cggatgcccc gcgcgcgcgc gccactcgcg atccggccgg tggcccccgc
     2221 cgcgctgctg gcccggagcg tcacaccgcg agtttgccac gaactcgctc gcgcatgggc
     2281 ggcgacgcgc ccgtgactcg gctccccgaa gcggccgccg atcgggcatc ctgggccggt
     2341 cgtcggcgct gtaacctgcc ccaccccgac ggcgttggga gtctgtccat gcgcctcgcc
     2401 ctgctcgccg tgtcctgcct cgtcgtcgcc tgtggcggcg gcgaacccgt ccagctcgcg
     2461 ccgcaggcgg agaagctgac gatcgaggcg ccgaagtcga ggggcgcgca gcgctacgtc
     2521 atcgccgcga ccggcggcaa gctcgagttc gacatggagg cgccgatcga gaagatccgc
     2581 gggcgtgtgc ccgaggccgc ggtcagcggc gagatcttcg tcgacgccag cgacctgacg
     2641 aagaccaccg gcctggtcca cgtcgacctc cgcgagctcg agctgttcca gcggacggcg
     2701 cccgccgagg gccagccctt cggcgaggag acccgaagcg acaagcagaa cgagcacgcc
     2761 cgggcctggc tggagatcgg cgcggacacc ccggaggacg tgctcaagaa gaacacgctc
     2821 gtcgagttct cgctgcgcga ggtcaaggac gcctccgcga ccgacctgag caagctgacc
     2881 ggccccgagc gcaaggtgac cttcaccgcg accggcgagt tcctgctgca ccagcgcaag
     2941 gccagcaagt cggcgaagct cgaggcgacc ttcacgtggg ccggtgacgc gccgacgggg
     3001 atcacgatca agaccctcga gcccctcgag atcggcctcg aggagtacga cgtgcgcccg
     3061 cgcgagggct tctcgaagct cgccgccaag acgctgcagg ccctctcgcc caaggtcgcc
     3121 agcgcggcgc aggtcaactt cgagtttacc gcgaagctcg ccccggacgc caagccgacg
     3181 atgcagtcgg tcgccgccga agcgacgccc accacgccgc cggcccccgc ggcccccgcc
     3241 ggctgacacg ccgatccatc tccccccgtc gtaacgtaca agcacaggaa gcacctcatg
     3301 gacatccgca ccatcaccgc caccctcgcg atcctcggca ccggcctgac cctcaccgcc
     3361 tgcaagaagt cctcctccga cgcctccgag gtgcccgcct cgcagcagcc cgcgggcgag
     3421 ggctcgtgcg gccccgacaa gggccacggg cacgccaact gcagcggcga caagaccgac
     3481 gcgcccgcca gcgagccgac cggcgacgac gcggccgcgc
//
LOCUS       DASWNJ010000056         2410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_142413, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000056 DASWNJ010000000
VERSION     DASWNJ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142413"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VGB85_01075"
     CDS             complement(<1..701)
                     /locus_tag="VGB85_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0132628.1"
                     /translation="MSIGAYDIRVVNDMVERESAFVERLLTEIHKVVVGQDEMIERVF
                     MGLLCGGHVLLEGAPGLAKTLTVATLARALKMDFKRVQFTPDLLPSDVLGTVIYNHQS
                     GSFTNRRGPVFTNLLLADEINRAPAKVQSALLEAMAERQVTIGDTSYPLPRPFLVLAT
                     QNPIEQEGTYTLPEAQLDRFMFKIKVGYPTEAEEEQIVRQETSDYQADLKRVLSPQRI
                     LELQDLVRRVPVADHV"
     gene            935..1357
                     /locus_tag="VGB85_01080"
     CDS             935..1357
                     /locus_tag="VGB85_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132629.1"
                     /translation="MVISRQFERLFDLRTIRRNIRSGNMTKEKYEEYLRSLPDMADNI
                     APLSSMDDDGEGKPGRGEGHGSPTPSFPRRTDVPILSGDDDDDMDDDDLDDDDDDDDD
                     TDEKVSAAPRIAAAAPDADTTASPDAGISPDLGDDQPE"
     gene            1569..>2410
                     /locus_tag="VGB85_01085"
     CDS             1569..>2410
                     /locus_tag="VGB85_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY0132630.1"
                     /translation="MVSSLPPESRRKGRRLRRGVGSGMVVRADGQVLTNYHVIAEAVS
                     FAIEYADGTRVPARQIHADPRLDLALIAPEVAQTDRTPARLSERRPRPGEWVMAIGHP
                     FGLGDTVTVGVISGLGRDHDDLGRPEELDPDGVWSFIQTDASINRGNSGGPLVDLRGE
                     VIGLTTAVRSDGQGVAFAVPAPMARKFLNEVWAHGRFRHARLGVDVSELDEGPGVRVM
                     TVVADGPGDRGGLRPGDLLLTANDAPLLRVSDLAYLGRVSGVGEPLTLQLQRGATRLS
                     LQIVP"
BASE COUNT          356 a          874 c          837 g          343 t
ORIGIN      
        1 acgtgatcgg cgacgggcac ccgtcgcacc agatcctgca gctcgaggat ccgctgcggt
       61 gacagcaccc gcttgaggtc agcctggtag tccgaggtct cctggcggac gatctgctcc
      121 tcctcggcct cggtcgggta gccgaccttg atcttgaaca tgaagcggtc gagctgggcc
      181 tcgggcagcg tgtaggtgcc ctcctgctcg atcgggttct gggtggcgag caccaggaag
      241 ggcctcggca gcggatagct ggtgtcgccg atcgtcacct ggcgctcggc catcgcctcg
      301 agcagcgcgc tctggacctt ggccggcgcg cggttgatct cgtccgccag cagcaggttg
      361 gtgaacaccg ggccgcgacg gttggtgaag ctgcccgact ggtggttgta gatcaccgtg
      421 ccgagcacgt ccgagggcag caggtccggc gtgaactgca cgcgcttgaa gtccatcttc
      481 agcgcgcgcg cgagggtcgc gaccgtcaag gtcttggcga gaccgggcgc gccctcgagc
      541 aacacgtgac cgccgcacag caggcccatg aagacccgct cgatcatctc gtcctgaccg
      601 acgacgacct tgtggatctc cgtcagcagg cgttcgacga acgcgctctc gcgctcgacc
      661 atgtcgttga cgacccggat atcgtaggca ccgatgctca tggactgctc tctggaggct
      721 cgggttggcg gcggagagta tatgcgccgc ggccggaaac catgtccggc gggccgctat
      781 tcctcatccc gcacgcccgc ggctccgggc gggtcgggga cgtgagctga ctcgccagta
      841 cggccgggca ccggcggcag cggcgagcgc gcgggccggg aagcgctcgc cggtttgacg
      901 gcggggggtg cctggtctac aaggacagcc gcccatggtt atctcgcgcc aatttgaacg
      961 gctcttcgat cttcggacca tccgccgcaa catccgcagc gggaacatga ccaaggagaa
     1021 gtacgaggag tacctccgct ccttgccgga catggccgac aacatcgcgc cgctgagcag
     1081 catggacgac gacggcgagg gcaagccggg ccgcggcgag ggccacgggt ccccgacgcc
     1141 ctcgttcccg cgccgcaccg acgtgccgat cctcagcggt gacgatgatg acgacatgga
     1201 cgacgacgac ctcgacgacg acgacgacga cgacgacgac acggacgaga aggtcagcgc
     1261 cgccccgcgg atcgctgccg ccgcccccga cgccgatacg accgcctcgc ccgacgccgg
     1321 catctcgccc gacctcggcg acgaccagcc ggagtgagcg gcgaaggcca gccgtcgcgc
     1381 tggcctggct cgctgtctgc ctggcgacct gcctggccac cctcgcctgc cgccccgcgg
     1441 aggcccccca gcgccgcgcc gaaccctccc cggaggtcgc ggcgccggcc gagccggtcg
     1501 cgccggccac tacatcgctg cccgacttcg ccgagctcgc ctcgcgcctg agcccgacgg
     1561 tcgtcagcgt ggtctcgtcg ctgccaccgg agagccggcg caagggccga cgactgcgcc
     1621 gcggtgtcgg cagcgggatg gtggtccgcg ccgacggcca ggtgctgacc aattatcatg
     1681 tgatcgccga ggcggtcagc ttcgcgatcg agtacgcgga cggcacccgc gtgcccgcgc
     1741 gacagatcca cgccgacccg cgcctcgacc tcgcgctgat cgcccccgag gtggcccaga
     1801 ccgaccgcac cccggcccgg ctcagcgaac gccggccgcg acccggcgaa tgggtgatgg
     1861 cgatcgggca ccccttcggg ctcggcgaca ccgtcacggt cggggtgatc tccggcctcg
     1921 gccgcgatca cgacgacctc ggccgccccg aggagctcga tcccgacggc gtctggagct
     1981 tcatccagac cgacgcgtcg atcaatcgcg ggaactccgg cgggccgctc gtcgacctgc
     2041 gcggcgaggt catcggcctg acgacggccg tgcgcagcga cggacagggc gtcgccttcg
     2101 ccgtgcccgc cccgatggcc cgcaagtttt taaatgaggt gtgggcccac ggccgcttcc
     2161 ggcacgcgcg gctcggcgtc gatgtcagcg agctcgacga ggggcccggc gtgcgcgtga
     2221 tgaccgtcgt cgccgatggg cccggcgacc gcggcgggct gcggcccggc gacctgctgc
     2281 tgacggccaa cgacgcgccg ctgctgcggg tgtcggacct cgcgtatctc ggccgcgtca
     2341 gcggcgtcgg cgagcccctg accttgcagc tgcaacgcgg agcgacccgc ctgtcgctgc
     2401 agatcgtgcc
//
LOCUS       DASWNJ010000057         2611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_637147, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000057 DASWNJ010000000
VERSION     DASWNJ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637147"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VGB85_01090"
     CDS             <1..413
                     /locus_tag="VGB85_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0132631.1"
                     /translation="MRAEQAALRAQVPAEPVRVYLEWWPRPMFSPGAACYSNELIALA
                     GGINVFADRAAASVEISTEELLRADPAVCFVSWCGVVEDKLDPGNLISRPGLEGLAAA
                     RAGRVYRLDERFSGRPGPRMLEAARVMARAIAEL"
     gene            465..758
                     /locus_tag="VGB85_01095"
     CDS             465..758
                     /locus_tag="VGB85_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132632.1"
                     /translation="MQVVSLEPQVARLVTELAQYRGYRTLWLDRRGYLCHSEPEDDYE
                     DIGFAYVATVFSPDADELSKTLASFAARRASRVGAWPVAAAIRMSPIAALAPA"
     gene            complement(794..2137)
                     /locus_tag="VGB85_01100"
     CDS             complement(794..2137)
                     /locus_tag="VGB85_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132633.1"
                     /translation="MLARLVVATATLLTPVLARAAPIEFVEQRRNFMVFNDVDGTVEN
                     ANWFARESFYQGYYPIEEGFLKAHPDDSQFIVVYSTFKLVQGVGAFYQSLANDVAGIG
                     YQKAADLDPIIPEEFFDDTPDSQFQGMLHMNDWHNFMLPGNTGFNDQWISLVFGQELG
                     HAWLAFVHADLGEGPSDKMLGRAKAHWSFYLHSDGSPVEGHRWTDNGDGSFTAAKLTE
                     YQFSDLDLYLMGLMGPDEVEPWFLIEDPHDCPDSNLEDKACADPSGHGFKADQYRVHG
                     TRRDITIKDVINVEGPRMPAYPDAPNAFDLSFILIKRPDEVLCDDELAAIDEIVDRSI
                     AEWVGQTRGRATLVNRTHVDAEPEPGPACETPVDSESSSSSDTGDAASAGEDTGPTPT
                     TGNNDTSSTAPTSEGSESSEGGQQTETSCACRNAPAGPWLLSTLLLPLLRRRRRA"
     gene            complement(2152..>2611)
                     /locus_tag="VGB85_01105"
     CDS             complement(2152..>2611)
                     /locus_tag="VGB85_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132634.1"
                     /translation="HWLPDHPKALAHLAAMLRPGGQLAVQIPYNQHHPGHVLAATLAA
                     EPPFCDDLEGFVQRFGVLPPAEYLVLLDRLGFTRPHVRLQVYPHRLGEGAAVADWLSG
                     SLLTGYRERLSPERYREFFVEYRRRVAAALGEGQPYLYAFERLLFWARKP"
BASE COUNT          361 a          776 c         1042 g          432 t
ORIGIN      
        1 agatgcgggc cgagcaggcc gcgctgcggg cccaggtccc ggcggagccg gtgcgcgtgt
       61 acctcgagtg gtggcctcgt ccgatgttca gtccgggggc tgcgtgctac agcaacgagc
      121 tgatcgcgct cgcgggtggc atcaatgtct tcgcggacag ggccgccgcc agcgtcgaga
      181 tctccaccga ggaattgctg cgggccgacc cggcggtgtg cttcgtgtcg tggtgcgggg
      241 tcgtggagga caagctcgac cccggcaacc tgatctcgcg gccgggcctc gaggggctgg
      301 cggccgcgcg ggccgggcgg gtgtaccgcc tggacgagcg cttctcgggc cgtccagggc
      361 caaggatgct cgaggcggcg cgcgtgatgg cgcgggcgat cgcggagcta tagaagcttc
      421 gccgtttttt tgtgtcggcg atcgcggcat gtcatgggtg cgccatgcag gtcgtgtcac
      481 tggagcccca ggtcgcccgt ctcgtcaccg agctcgccca gtaccgcggg tacaggacgc
      541 tgtggctcga ccgtcgcgga tatctctgtc acagcgagcc cgaggacgac tacgaggata
      601 tcggcttcgc ctacgtcgcc acggtgttct cgccggacgc cgacgagctg agcaagacgc
      661 tcgcctcgtt cgccgcccgt cgcgcctcgc gcgtcggcgc ctggccggtc gcggctgcga
      721 tccgcatgag cccgatcgcc gcgctcgcgc cggcgtgagg cattgtccgt acctggccta
      781 aaggccaggt gtctcaggca cgacggcggc ggcggagcag gggcaggagc agcgtcgaga
      841 gcagccaggg gccggcgggc gcgttgcggc aggcgcagct ggtctcggtc tgctggccgc
      901 cctcgctgct ctcgctgccc tcgctggtgg gggcggtcga gctggtgtcg ttgttgccgg
      961 tggtcggggt cgggccggtg tcttcgccgg cgctggcggc gtcgccggtg tcgctgctgc
     1021 tcgagctctc ggagtcgacg ggcgtctcgc aggcggggcc gggttcgggt tcggcgtcga
     1081 cgtgggtgcg gttgacgagg gtggcgcggc cgcgggtctg gccgacccac tcggcgatcg
     1141 accggtcgac gatctcgtcg atggcggcga gctcgtcgtc gcacaggacc tcgtcgggac
     1201 gtttaataag gatgaaggag aggtcgaagg cgttgggggc gtcggggtag gcgggcatcc
     1261 gcgggccctc gacgttgatg acgtccttga tcgtgatgtc gcggcgggtg ccgtgcacgc
     1321 ggtactggtc ggccttgaag ccgtggccgg aggggtcggc gcaggccttg tcctcgaggt
     1381 tggagtcggg gcagtcgtgc gggtcctcga tcaggaacca tggctcgacc tcgtcggggc
     1441 ccatcaggcc catgaggtag aggtcgaggt cgctgaactg atactcggtg agcttggcgg
     1501 cggtgaagct gccgtcgccg ttgtcggtcc agcgatggcc ctcgacgggg gagccgtcgc
     1561 tgtgcaggta aaagctccag tgggccttgg cgcggccgag catcttgtcg ctcggaccct
     1621 cgccgaggtc ggcgtgcacg aaggcgagcc acgcgtggcc gagctcctgg ccgaacacga
     1681 gcgagatcca ctggtcgttg aagccggtgt tgcccggcaa catgaagttg tgccagtcgt
     1741 tcatgtgcaa catcccctgg aactggctgt cgggggtgtc gtcgaagaat tcttcgggga
     1801 tgatcgggtc gaggtcggcg gccttttgat agccgatgcc ggcgacgtcg ttggcgagcg
     1861 actgataaaa ggcgccgacg ccctggacca gcttgaaggt gctgtagacg acgatgaact
     1921 ggctgtcgtc ggggtgggcc tttagaaagc cctcctcgat cgggtagtag ccctgataaa
     1981 acgactcgcg cgcgaaccag ttggcgttct cgacggtgcc gtcgacgtcg ttgaagacca
     2041 tgaagttgcg gcgctgctcg acgaactcga tcggcgcggc gcgggcgagg accggcgtca
     2101 ggagcgtggc ggtggcgacg acgaggcggg cgagcacgcg ccgagcatac gtcatggctt
     2161 gcgggcccag aacagcaggc gctcgaaggc gtagaggtag ggctggccct cgccgagcgc
     2221 ggcggcgacg cggcggcggt actcgacgaa gaattcacgg tagcgctcgg ggctgaggcg
     2281 ctcgcggtag ccggtcagca ggctgccgga cagccagtcg gcgaccgcgg cgccctcgcc
     2341 gaggcggtgg gggtagacct gcaggcggac gtgcgggcgc gtgaagccga ggcggtcgag
     2401 caggaccagg tactcggcgg gaggcagcac gccgaagcgc tggacgaagc cctcgaggtc
     2461 gtcgcaaaac ggcggctcgg cggcgagggt ggcggcgagc acgtggccgg ggtggtgctg
     2521 gttgtagggg atctggacgg cgagctggcc accggggcgc agcatcgcgg cgaggtgggc
     2581 gagggccttc gggtggtcgg gcagccagtg c
//
LOCUS       DASWNJ010000058         2137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_460535, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000058 DASWNJ010000000
VERSION     DASWNJ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460535"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VGB85_01110"
     CDS             complement(<1..183)
                     /locus_tag="VGB85_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132635.1"
                     /translation="MTVIFCHGLESSPHGRKYQALVEAGLDVISPDFQGQDLAARVAT
                     LEPVLRGAVDPVLVGSS"
     gene            278..>2137
                     /locus_tag="VGB85_01115"
     CDS             278..>2137
                     /locus_tag="VGB85_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEY0132636.1"
                     /translation="MDEPPRPPEPAGPTPGDPPASTSRPQAPRIEVDVQSTGFFKMLQ
                     SGTRLAGLWSRFGLLLGRQLAVVIRDDHVFLILMAAVVGITSGAAAGALLAWIAYAIE
                     LFPRGETGDSVMRWVFVLGVPVLGGLLAGGLHLVLRRLIAQPPVAGIPAVIEAVARGG
                     GRIGGRQAVVVGLGTGITIGSGGSCGHEGPSVALGAAVGSVLSRFFGLRMRWHLSMVG
                     AGCAGGLAAAFNAPLAGVIFTVEIVFGGAIGGNVGTMSVFVPLIVAAVAGTLTSHAIR
                     GEHLTFQTVQHGEGMLAEMLFFVALAALAGLVGTAFGRSMIATTRWFDRLRVPTWVKP
                     AIGALGVGLLAALVSNELLGPGHPTLARALDGSLGWKLALALGSLKMVATALTVGSGG
                     FGGVFMPSLYVGACLGTLVGVLARLALGPGVESVGAYALVGMGAIFAATMHAPLTPIV
                     MIFELTQDYSIILPLMLGCILSVFVARRTYPLSYFKEQLRVRGVILGREAEVEVMKRG
                     HVRELMQPPGSVLPPTADLETVRKATLDADLRATFVCDEQGAVVGFVNGNQLARRMLN
                     GEINAASTAADLMGTAGLPFLYPGDTLAGAMLASARSGMEFLPVVDDERRLLGV"
BASE COUNT          243 a          728 c          827 g          339 t
ORIGIN      
        1 gctcgagccg acgagcaccg ggtcgacggc gccgcgcagc acgggctcca gggtggcgac
       61 ccgcgcggcg aggtcctgac cttgaaagtc cggcgagatc acgtcgaggc ccgcctcgac
      121 cagcgcctgg tacttgcggc cgtgcgggct gctctcgagg ccgtggcaga agatgacggt
      181 catgcgaggc gcaggctaac ccgggtgcgc gacctcggcc acccgcggcg agaccggcga
      241 ggaaaccggc gccagacttg ttactgtatc cgctgcgatg gacgagccgc cgcgcccacc
      301 cgagcccgcg ggaccgaccc cgggcgaccc gccggcgagc acgtcgaggc cgcaggcgcc
      361 gcgcatcgag gtcgatgtcc agtcgaccgg cttcttcaag atgctgcaga gcggcacgcg
      421 gctcgcgggg ctgtggtcgc gcttcgggct gctgctcggt cgccagctcg cggtcgtgat
      481 ccgcgacgac cacgtgttcc tgatcctgat ggcggcggtc gtcggcatca ccagcggcgc
      541 ggcggcgggc gccctgctgg cgtggatcgc ctacgcgatc gagctgttcc cccgcggcga
      601 gaccggcgac tcggtgatgc gctgggtgtt cgtgctcggc gtgccggtgc tcggcggtct
      661 gctggcaggc gggctgcacc tggtgctccg gcggctgatc gcgcagccgc cggtcgccgg
      721 gatcccggcc gtcatcgagg cggtcgcgcg cggcggcggg cggatcggcg ggcgccaggc
      781 ggtcgtcgtc ggcctcggca ccgggatcac gatcggcagc ggcgggtcgt gcgggcacga
      841 ggggccgtcg gtcgcgctcg gggccgcggt cggcagcgtg ctgtcgcgct tcttcgggct
      901 gcgcatgcgc tggcacctgt cgatggtcgg ggccggctgc gcgggcggcc tggcggcggc
      961 cttcaacgcg ccgctggcgg gcgtgatctt caccgtcgag atcgtgttcg gcggggcgat
     1021 cggcggcaac gttgggacga tgtcggtgtt cgtgccgctg atcgtcgcgg cggtcgccgg
     1081 cacgctgacc tcgcatgcca ttcgcggtga gcacctgacc tttcagacag tccagcacgg
     1141 cgagggcatg ctcgccgaga tgctgttctt cgtcgccctc gcggcgctgg cgggcctggt
     1201 cggcacggcc ttcgggcgct cgatgatcgc gacgacgcgg tggttcgacc gcctgcgcgt
     1261 gccgacgtgg gtcaagccgg cgatcggggc gctgggcgtc ggcctgctcg ccgcgctggt
     1321 gtccaacgag ctgctcggcc ccggccaccc gacgctggcg cgcgcgctcg acggcagcct
     1381 cgggtggaag ctggcgctcg cgctcgggtc gttgaagatg gtggcgacgg cgctgacggt
     1441 cggcagcggc ggcttcggcg gggtgttcat gccctcgctg tacgtcggcg cgtgcctggg
     1501 cacgctcgtc ggcgtgctcg cccggctcgc gctcgggccc ggggtcgaga gcgtcggcgc
     1561 ctacgcgctc gtcgggatgg gggcgatctt cgcggcgacg atgcatgcgc cgctgacccc
     1621 gatcgtgatg atcttcgagc tgacgcagga ctactcgatc atcttgccgc tgatgctcgg
     1681 ctgcatcctg tcggtgttcg tcgcccgccg aacctacccg ctgagctatt ttaaagagca
     1741 gctgcgggtc cgcggcgtga tcctcgggcg cgaggcggag gtcgaggtga tgaagcgtgg
     1801 ccacgtgcgc gagctgatgc agccgcccgg ctccgtgctg ccgccgacgg ccgacctcga
     1861 gacggtgcgc aaggcgaccc tcgacgccga cctgcgcgcg accttcgtct gcgacgagca
     1921 gggtgcggtg gtcggcttcg tcaacggcaa ccagctcgcg cgcaggatgt tgaacgggga
     1981 gatcaacgcg gcctcgaccg cggccgacct gatgggcacc gccggcctgc cgttcctgta
     2041 ccccggcgac acgctcgcgg gcgcgatgct ggcgtcggcg cgctcgggca tggaattctt
     2101 gccggtcgtc gacgacgagc ggcgcctgct cggcgtg
//
LOCUS       DASWNJ010000059        11482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_495372, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000059 DASWNJ010000000
VERSION     DASWNJ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 11482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11482
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495372"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VGB85_01120"
     CDS             complement(<1..695)
                     /locus_tag="VGB85_01120"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0132637.1"
                     /translation="MSSQNIDALVDRGLAAVEADDLETATQILEDATKLGGENHVRVL
                     HLTGMLAWAEGRPEHAAGYLMQAVDAGSEDANIYLDCAECLLLHDQDLDEAEAAARTV
                     LRLGGASLEAQDQARLLLSQIRLADEDTEEALELLDGISDSRKQDPVFLSTYGFVLMN
                     AGRDEEAVTALRSAVEADPEDPDVHYWLGQALEGVGDVTGSRASMLKVLELDQAEHDD
                     ADHEHEPLTSEIE"
     gene            762..1295
                     /locus_tag="VGB85_01125"
     CDS             762..1295
                     /locus_tag="VGB85_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132638.1"
                     /translation="MPAPEPTKDVSPLMDMLRERWVLTLATSPGANAVDPEPRIPYPT
                     PLFYAVADLPGPPLLVFASRPDSTHGRHLGDGPTAVAAGLYLESEAVAELRGVQLRGE
                     VVRVAGAPAAAGLIPVLRAAYVGRHPAADALLRPGARDELYVLAVTWAKLTDNRRGFG
                     HKLEWRFTTPEVFFAGP"
     gene            1330..1953
                     /locus_tag="VGB85_01130"
     CDS             1330..1953
                     /locus_tag="VGB85_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132639.1"
                     /translation="MLTRRSWLGLMAVGACTLAARPAWAAAADSPDHAGVVEVAAANN
                     AVGAIIAAEGGTAVQVVVDPKLGPLQIRIDGAVVDVSGTILLKGRFLDDPRNASKLGA
                     SVRNALVAAKPALKATLEANHKAWTHPFARRVIAWNAKLAKSSVRGQRVADVHGRAAL
                     LEWAGAVIDAKSAVKGPAALASAPKGPAAPTLESYIEYLEALVRTVA"
     gene            2228..4156
                     /locus_tag="VGB85_01135"
     CDS             2228..4156
                     /locus_tag="VGB85_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEY0132640.1"
                     /translation="MIRFAGDSGDGMQLTGSQFSESTALMGNDLSTLPDFPAEIRAPA
                     GTTFGVSGFQVHFAGHDIKTPGDAPDVLVAMNPAALRVNLRDLKTGGMLVVNTGAFTT
                     GNLKKAGYAVTPLDDGSLDAFRVLKIDISKLTLQAVAPFNLGTKEALRAKNMWTLGLM
                     MWMFGRERESTVEWLRQKFAKNPAIADANVAALNAGHVYGETAEMPGGIGCYQVPKAK
                     LDPGVYRNVTGNEATAWGLLTGGKLAGLELVLGSYPITPASTILHTLSNLRAYGVTTF
                     QAEDEIAAICAAIGASYAGALGMTSTSGPGLALKAEALGLAVATELPLVVVDVQRGGP
                     STGLPTKTEQSDLFQALNGRNGDCPLAVLAACTPGDCFDMAIEAVRIATKYMTPVILL
                     TDGFLANSAEPWKIPDITTIKPFPVQHRRDPEGFHPFLRDPTTLARAWAVPGTPGLEH
                     RIGGIEKDYNSGNISYDPDNHHKMTKVRAAKIAGIADDIPLQKVEQGDDTGDLVVVGW
                     GSTYGAISQAVLGARRSGFKVSHVHIRYMNPMPKNLGELLRGFKQVLVPEMNNGMLVK
                     VLRAEYLVDAKGLNKIAGHPFKIAEIEAEIRAKLGHPSSEPAGHYNLGHENIGNNTAG
                     TVGDNLGNNNVIPLVSEA"
     gene            4161..5207
                     /locus_tag="VGB85_01140"
     CDS             4161..5207
                     /locus_tag="VGB85_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEY0132641.1"
                     /translation="MTPLDPPNPPLTRKDFTSDQEVRWCPGCGDYAILAAVQRMLPEL
                     GVRKENTVFVSGIGCSSRFPYYMDTYGFHTIHGRAPAVATGVKLANPDLDVWMITGDG
                     DGLSIGGNHLMHVLRRNVNMQLILFNNRIYGLTKGQYSPTSLLGKRSPSTPMGSVDNP
                     LIPAAFALGCGGRFVARAIDTDAKNLIATLKRAHEFVGTGFVEVYQNCPVFNDGAFEH
                     FTAREIAAETQLHLEHGKPMRFGKDHTKGLRLKPGQLELEVVTLGEYGVTEADILVHD
                     EHNHTMAYLLATLEPPSFPVALGVLYRAPGPDASFERGMHEQNRQARAKGVGDLNSLM
                     RKGHTWLVEPEPTT"
     gene            5444..6334
                     /locus_tag="VGB85_01145"
     CDS             5444..6334
                     /locus_tag="VGB85_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132642.1"
                     /translation="MRLSNFHFGRLAALTGFLTSAAIGCVLTVGGGKAADGESCPDTN
                     SYIKEGECFCDVGYDWCDENDNTDLTCCENPTTGNTNSTNNNTNNTDQTNGTTEDVPT
                     SGTSDTTTDVPTSGTTGDNLECTVDTAPPAACNPDTEPFLCIQASNPACDTEGSKFYV
                     CTGGAWVEDPNGPDANCKADGYDFGVGCEDDGTKIVFDCGVGPGTPCQTGADPSCNGE
                     TIYEECYHGKLTATDCEAYCMEVGDDMMVTYDYGYCGEQRSEIACICCDEGDEGCPIN
                     EGTTGGDTTTDGGTTGTTGG"
     gene            complement(6412..6690)
                     /locus_tag="VGB85_01150"
     CDS             complement(6412..6690)
                     /locus_tag="VGB85_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132643.1"
                     /translation="MAADDVPSSRDLHPVAGARFVCERAEGDELVYRVAVHAAGGHTH
                     HAELRWDAAGQATATPPHADAWAQAELLKLARVLKHSQQDRLTRWRGP"
     gene            complement(6690..7457)
                     /locus_tag="VGB85_01155"
     CDS             complement(6690..7457)
                     /locus_tag="VGB85_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132644.1"
                     /translation="MPPATPRLFVASLALLALAPTGAAASPYRILAPAPAAGAGAPPV
                     LPASPSDSAAPTDSAAIAAATSPTQPLSSDPALEYRRGEQAYALGNYEQAVRHFEHSY
                     QLSNFADLLYNIGLAHAQWHGLDGDIGHLRKARRLFQNYIKRLAENPAMDQSQRAEAE
                     AQVAKIDEQISEHEAKQAAVVPPPVVEPDRAPKPAPPVDVPERRPVHKRGWFWGVIGG
                     IVVIGAVTAAVVLTRKPGFEPELGTIGPTSSAVGLRF"
     gene            complement(7518..8747)
                     /locus_tag="VGB85_01160"
     CDS             complement(7518..8747)
                     /locus_tag="VGB85_01160"
                     /inference="COORDINATES: protein motif:HMM:NF021599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 family protein"
                     /protein_id="HEY0132645.1"
                     /translation="MQSGADTQEWVTSLARQATFARGYSPLSAAILDCLGACIRGEVS
                     GAGESSGTPGEQSRLRERFAKFVSSRAWDNDLEPILKLAATLHSHVLREDPRVAGLAR
                     YYATVTAEPASPEDPEFAGELLRALDSLGDDLLREADRWLIQTNETARGVLWLLPAAL
                     LQIDAAYLVELGASAGLNLLADLRRYDLRWNDGAPLRLGQGETWQFELDCDGPRPALP
                     SIVKPPEILARVGADLRIVDLDEPGAETELAACIWGDQPRRMQRLQEALALRRELRDN
                     GQGARLLKTRLPDETEDFLRAVVPIHPVAPVILFDTYVTAYFNDVDHRALVRAVEAFA
                     RPWTLRHKLSWLWVRFEPPRAGEPEAPQIGWCRWWVEVWTQGQRHTLELGWAHPHCVK
                     LVFGPGLSELTRLRGPE"
     gene            8810..10024
                     /locus_tag="VGB85_01165"
     CDS             8810..10024
                     /locus_tag="VGB85_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY0132646.1"
                     /translation="MQVPLRPLEAASWASSVVPGARYDLSSSTGPGTRGSLGAALPRT
                     PDETGEDETAASWDEAEASLVHAIASRYGVDPIQVALTPGAGQAIVHALIAMLRPGDH
                     VVVERPTYEPLHRVPEMLGASVARIDRRIEDDWQLLPERLAKVLTPRTRAVVLTNLHS
                     PSGVACSPRTIAEIAGLAARVGATVLVDEVLLDHAFDLGPAAACRPACLVADNAICFS
                     STSKGLGLPELRVGWLVSRDPDAARGLRIAADYLHRRLPPATMRLAAHALRDPDDFRI
                     RTARLAGAGRRVVERWVQGEPRVEWIPPHAGLHTLVRLPVGVSDLAFAAHLRARYETQ
                     VIPGSVLEAPQCVRLGFAAPSSTLEQGLANFSAALDDLLGGSTLAGPGLAPDRPLLGV
                     VPLRDDDDPGAA"
     gene            complement(10025..11125)
                     /gene="ychF"
                     /locus_tag="VGB85_01170"
     CDS             complement(10025..11125)
                     /gene="ychF"
                     /locus_tag="VGB85_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEY0132647.1"
                     /translation="MALAVGIVGLPNVGKSTLFNALSNAGAQSANYPFCTIEPNVGMV
                     SVPDPRMEALVKCVEPNKIVPTTVEFVDIAGLVKGASQGEGLGNQFLTHIRNVDAIVH
                     VVRCFEDDNIIHVNNKVDPVGDAEIINTELLLRDMQSVEARLNKAKKASKSGDKTEKL
                     ALVSCEKLMDAMGRGIMVRNIPQDDFDRELIQGLALLTAKPALYAANVSEAQLAAGLT
                     DPLVARLVEVATREGTEVVVISAAIEAEISGLSPDERREFLEGIGAEEPGLSRLIRRA
                     YALLDLITYFTAGKQEVRAWTIQRGTLAPGAAGKIHSDFERGFIRAEVMNWNDLVTLG
                     SEAAVKSKGLMRVEGKEYTVQDGDVMHFRFNV"
     gene            complement(11196..>11482)
                     /locus_tag="VGB85_01175"
     CDS             complement(11196..>11482)
                     /locus_tag="VGB85_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132648.1"
                     /translation="LVPIARGAIGGGKWWYPDIGGSRQQARESTFAMSVRPGGGLRFF
                     PILDLGIGIDVNFVLGFSVSKDTPLNQPTTKKAGFLFGIEILPLIIEYRF"
BASE COUNT         1739 a         4290 c         3757 g         1696 t
ORIGIN      
        1 tcctcgatct cgctggtcag cggctcgtgc tcgtgatcgg cgtcgtcgtg ctcggcctgg
       61 tcgagctcga gcaccttgag catcgacgcg cgggagcccg tgacgtcacc cacgccctcg
      121 agcgcctggc cgagccagta atgcacatcc ggatcttccg ggtcggcctc gaccgccgag
      181 cgcagcgccg tgaccgcctc ctcgtcgcgg ccggcgttca tcagcacgaa gccgtaggtg
      241 ctcaggaaca cggggtcctg cttgcggctg tcgctgatcc cgtcgagcag ctcgagcgcc
      301 tcctcggtgt cctcatcggc gaggcggatc tgcgagagca gcaggcgggc ctggtcctgg
      361 gcctcgaggc tggcaccacc gaggcgcagc accgtacgcg cggccgcctc ggcctcgtcg
      421 aggtcctggt cgtgcagcaa caggcactcg gcgcagtcga ggtagatgtt cgcgtcctcg
      481 gacccggcgt cgacggcctg catcaggtaa ccggcggcgt gctccggacg accctcggcc
      541 cacgccagca tcccggtcag gtgcagcacg cggacatgat tctcgccgcc cagcttggtc
      601 gcatcctcca ggatctgcgt ggcggtctcg aggtcgtcgg cttcgactgc tgcaagtccg
      661 cggtcgacaa gggcatcgat gttttggctg ctcatggcgg cggactctag cacgggcacg
      721 ctcggccccg cgcgcgccac cggccatgct aaatcgctgc gatgcccgcc ccggagccga
      781 ccaaggacgt gagtccgctc atggacatgc tgcgagagcg ctgggttctg accctggcga
      841 cctcaccggg ggcgaatgcg gtcgacccgg agccgcgcat cccctacccg acaccgctgt
      901 tctacgcggt cgcggatctc cccggtccgc ccttgctggt gttcgccagc cggcccgact
      961 cgacccacgg acgccacctc ggcgacggtc cgaccgccgt cgccgccggg ctctacctcg
     1021 agtccgaggc ggtcgccgag ctgcgcgggg tccagctgcg cggtgaggtc gtccgcgtcg
     1081 ccggggcgcc cgctgcggcg ggcctgattc cggtgctgcg cgccgcctac gtcggccggc
     1141 acccggccgc cgacgccctg ctgcggccgg gcgcccgcga cgagttgtac gtactggcgg
     1201 tcacctgggc caagttgacg gacaatcgcc gcggcttcgg ccataaactc gagtggcgct
     1261 tcacgacccc tgaagttttc tttgcaggtc cctgaagatc tctcgaggtc gcgcgtctga
     1321 ggttcgaata tgctgacccg aagaagctgg ctcggtctga tggcggtcgg cgcgtgtacc
     1381 ctggcggcgc gcccggcctg ggctgccgcc gccgattcgc cggatcatgc cggggtagtc
     1441 gaggtcgccg ccgccaacaa cgccgtcggt gcgatcatcg cggcggaggg tggcacggcg
     1501 gtccaggtcg tcgtcgaccc gaagctcggc ccgctgcaga tccgcatcga cggcgcggtc
     1561 gtcgacgtct cgggcacgat cctgctcaag ggtcgcttcc tcgacgaccc ccgcaacgcc
     1621 agcaagctcg gcgccagcgt ccgcaacgcc ctcgtcgcgg ccaagcccgc gctcaaggcg
     1681 acgctggagg ccaaccacaa ggcctggacc caccccttcg cccgtcgcgt gatcgcctgg
     1741 aacgcgaagc tggcgaagtc cagcgtccgc ggccagcgcg tcgccgatgt ccatggtcgc
     1801 gccgccctgc tcgagtgggc cggcgccgtc atcgacgcca agtccgccgt aaagggcccc
     1861 gcggcgctcg cctcggcgcc gaagggtccg gcggcgccga cgctcgagag ctacatcgag
     1921 tacctcgagg cgctggtccg gaccgtcgcc tgatcttctc tcgcggtcgt acaacaatca
     1981 gggccgtgaa gacaacttgc acgctcgtga agcgacgagc gcgacctccc ggggcgccgc
     2041 gcatgcgtgc cgtgtctcac tcgcccggcc cccgtggctc ggctgacatc ggcggcggcg
     2101 ggcctatcat gagcgcgcgt cgcggctacg tcggattgtc cacgcgccgc gacgctgtcc
     2161 catatcagtc atggatacct tcgcaacgag ccattcaagc ccctcgcggg agctcatcga
     2221 ttcggtcgtg atccgcttcg ccggcgactc cggcgacggc atgcagctga ccggctcgca
     2281 gttctccgag tccaccgccc tgatgggcaa cgacctgtcg acgctgccgg acttccccgc
     2341 ggagatccgc gcgccggccg gcacgacctt cggcgtctcg ggcttccagg tccacttcgc
     2401 cgggcatgac atcaagaccc ccggcgacgc gcccgacgtg ctcgtggcga tgaaccccgc
     2461 ggcgctgcgc gtcaacctgc gcgacctcaa gaccggcggc atgctcgtcg tcaacaccgg
     2521 cgccttcacg accggcaact tgaagaaggc cggctacgcc gtcaccccgc tcgacgacgg
     2581 cagcctcgac gccttccgcg tgctcaagat cgacatctcc aagctgacgc tgcaggctgt
     2641 ggcgccgttc aacctcggca ccaaggaggc cctgcgcgcc aagaacatgt ggacgctcgg
     2701 cctgatgatg tggatgttcg ggcgcgagcg cgagagcacc gtcgagtggc tgcgccagaa
     2761 gttcgccaag aacccggcga tcgccgacgc caacgtcgcc gcgctcaacg ccggtcacgt
     2821 ctacggtgag accgccgaga tgccgggcgg catcggctgc tatcaggtcc ccaaggcgaa
     2881 gctcgatccg ggcgtgtacc gcaacgtcac cggcaacgag gcgaccgcgt ggggcttgtt
     2941 gaccggtggc aagctcgcgg gcctcgagct cgtgctcggc tcctacccga tcaccccggc
     3001 ctcgacgatc cttcacaccc tgtcgaacct gcgcgcctac ggtgtcacca ccttccaggc
     3061 cgaggacgag atcgctgcaa tctgcgcggc gatcggcgcc tcgtatgccg gcgcgctcgg
     3121 catgaccagc acctccggcc cgggcctcgc gctcaaggcc gaggcgctcg gcctcgccgt
     3181 cgcgaccgag ctcccgctcg tcgtcgtcga cgtccagcgc ggcggcccgt cgaccggcct
     3241 gccgaccaag accgagcaga gcgacctgtt ccaggcgctc aacggccgca acggcgactg
     3301 cccgctcgcg gtgctcgccg cgtgcacgcc gggcgactgc ttcgacatgg cgatcgaggc
     3361 cgtgcgcatc gccaccaagt acatgacgcc ggtgatcctg ctgaccgacg gctttttggc
     3421 caacagcgcc gagccgtgga agatccccga catcaccacg atcaagccgt tcccggtcca
     3481 gcaccgccgc gaccccgagg gcttccaccc gttcctgcgc gacccgacga cgctcgcgcg
     3541 cgcgtgggcg gtccccggca ccccgggcct cgagcaccgc atcggcggca tcgagaagga
     3601 ctacaactcc ggcaacatct cgtacgaccc ggacaaccat cacaagatga ccaaggtccg
     3661 cgccgcgaag atcgccggca tcgccgacga catcccgctg cagaaggtcg agcagggcga
     3721 cgacaccggc gacctcgtcg tcgtcggctg gggctcgacc tacggcgcga tctcgcaggc
     3781 cgtgctcggc gcgcgtcgca gcggcttcaa ggtctcgcac gtgcacatcc gctacatgaa
     3841 cccgatgccg aagaacctcg gcgagctgct gcgcggcttc aagcaagtcc tcgtgccgga
     3901 gatgaacaac ggcatgctcg tcaaggtgct gcgcgccgag tacctcgtcg acgcgaaggg
     3961 cctcaacaag atcgccggtc atccgttcaa gatcgccgag atcgaggcgg agatccgggc
     4021 caagctcggc cacccgtcga gcgagcccgc cggtcactac aacctcggtc acgagaacat
     4081 cggcaacaac accgccggca cggtcggcga caacctcggc aacaacaacg tcatcccttt
     4141 ggtgtcggag gcctgatttt atgactcccc tcgatccccc gaacccgccc ctcacgcgca
     4201 aggacttcac ctcggaccag gaggtccgct ggtgccccgg ctgcggcgac tatgcgatcc
     4261 tcgcggccgt gcagcgcatg ctgcccgagc tcggtgttcg caaagagaac acggtgttcg
     4321 tgtccggcat cggctgctcg agccggttcc cgtactacat ggacacctac ggcttccaca
     4381 cgatccacgg gcgcgccccc gcggtcgcca ccggtgtgaa gctcgccaac cccgacctcg
     4441 acgtctggat gatcaccggc gacggcgacg gcctcagcat cggcggcaac cacctgatgc
     4501 acgtgctgcg ccgcaacgtg aacatgcagc tgatcctgtt caacaaccgg atctacgggc
     4561 tgacgaaggg ccagtactcg ccgacctcgc tgctcggcaa gcgctcgccc tcgacgccga
     4621 tgggctcggt cgacaacccg ctgatccccg ccgccttcgc gctcggctgc ggcggtcgct
     4681 tcgtcgcccg cgcgatcgac acggacgcca agaacctgat cgccacgctc aagcgcgccc
     4741 acgagttcgt cggcaccggc ttcgtcgagg tctatcagaa ctgccccgtg ttcaacgacg
     4801 gcgccttcga gcacttcacc gcgcgcgaga tcgccgcgga gacccagctc cacctcgagc
     4861 acggcaagcc gatgcgcttc ggcaaggacc acaccaaggg cctgcgcctc aagcccgggc
     4921 agctcgagct cgaggtcgtc accctcggcg agtacggcgt caccgaggcg gacatcctcg
     4981 tccacgacga gcacaaccac acgatggcct acctgctcgc gaccctcgag ccgccgagct
     5041 tcccggtcgc cctcggcgtg ctgtaccggg ctcccggccc ggacgcctcg ttcgagcgcg
     5101 gcatgcacga gcagaaccgc caggcccggg ccaagggcgt cggcgacctc aactcgctga
     5161 tgcgcaaggg ccacacctgg ctggtcgagc ccgagccgac gacctgagcc cgcatccccc
     5221 gcacctccgg gtgccagggt cgcactggag ccgcctcggt cacggggcgg ctttttcgtg
     5281 cctgcttctc gcgccccggg gtatacgggc cacctccggc tggccgcgcc ttgcgccgga
     5341 cggggtcgga gaatcaagct tccccgaccg tcgtctcagc cccacgccgg ctccggccgc
     5401 gcgccccgtc gcctctacct ctcgaacttt caggaacata accatgcgtc tctccaactt
     5461 ccacttcggt cgtctcgctg ccctcaccgg cttcctcacc tccgcggcga tcggctgcgt
     5521 ccttactgtc ggcggcggca aggcggcgga cggcgagtcc tgccccgata cgaacagcta
     5581 catcaaggaa ggtgagtgct tctgcgacgt cggctacgac tggtgtgacg agaacgacaa
     5641 caccgacctg acgtgctgcg agaacccgac cacgggcaac accaacagca ccaacaacaa
     5701 caccaacaac acggaccaga ccaacggcac gaccgaggac gtcccgacct cgggcacctc
     5761 ggacaccacg acggacgtcc cgacctcggg caccacgggc gacaacctcg agtgcaccgt
     5821 cgacaccgcc cccccggcgg cttgcaaccc cgacaccgag cccttcctgt gcatccaggc
     5881 ctcgaacccg gcctgcgaca ccgagggcag caagttctac gtgtgcaccg gcggcgcgtg
     5941 ggtcgaggac ccgaacggtc cggacgccaa ctgcaaggcc gacggctacg acttcggcgt
     6001 cggctgcgag gacgacggca ccaagatcgt gttcgactgc ggcgtcggcc cgggcacgcc
     6061 ctgccagacc ggcgcggacc cgtcctgcaa cggcgagacc atctacgagg agtgctacca
     6121 cggcaagctg accgccaccg actgcgaggc ctactgcatg gaggtcggcg acgacatgat
     6181 ggtcacctac gactacggct actgcggcga gcagcgcagc gagatcgcct gcatctgctg
     6241 cgacgagggt gacgagggct gcccgatcaa cgagggcacc accggcggcg acaccaccac
     6301 cgacggcggc accactggca ccaccggcgg ctgagctccc cgggcccaat cgaccgaggg
     6361 cggcgcgcga gcgaccgccc tcttcgttct taaggaaaga tagagagccg ctcagggccc
     6421 gcgccagcgg gtcaggcggt cctgctggct gtgcttgagc acgcgcgcca gcttgagcag
     6481 ctcggcctgc gcccacgcgt ccgcgtgcgg cggtgtcgcg gtcgcctggc ccgcggcgtc
     6541 ccagcgcagc tcggcgtggt gcgtgtgccc gccggccgcg tgcacggcga cgcgatacac
     6601 gagctcgtcg ccctcggcac gctcgcacac gaagcgggcc ccggcgacgg ggtgcaggtc
     6661 acggctcgat ggaacgtcgt cggccgccat cagaaccgta gccccaccgc gctggaagtc
     6721 gggccgatcg tgcccagctc gggctcgaag cccggcttgc gggtcaacac gaccgccgcc
     6781 gtgaccgcgc cgatcaccac gatcccgccg atcacgcccc agaaccaccc gcgcttgtgc
     6841 accggcctgc gctccggcac atcgaccggc ggcgcgggct tcggcgctcg atccggctcg
     6901 accaccggcg gtggcaccac ggccgcctgc ttcgcctcgt gctcgctgat ctgctcgtcg
     6961 atcttcgcga cctgcgcctc ggcctcggcc cgctggctct ggtccatcgc cgggttctcg
     7021 gccaggcgct tgatgtagtt ctgaaacagc cgcctggcct tgcgcaggtg cccgatgtcg
     7081 ccgtcgaggc cgtgccactg cgcgtgcgcg aggccgatgt tgtagagcag gtccgcgaag
     7141 ttcgagagct gataactgtg ctcgaagtgc cgcaccgcct gctcgtagtt gccgagcgcg
     7201 taggcctgct cgccgcggcg atactcgagc gccgggtcgc tcgacagcgg ctgcgtcggc
     7261 gaggtggccg ctgcgatcgc ggcgctatcg gtcggcgcgg cgctgtccga cgggctggcc
     7321 ggaaggacag gcggcgcccc ggcgcccgcc gcgggtgccg gcgccaggat gcggtacggg
     7381 ctcgccgcgg cgcccgtcgg cgcgagcgcc aggagggcga gcgacgcaac aaacaaccgt
     7441 ggcgtggcgg gcggcatggc tcgggcggac gatgccacca ccgcgcctcg ctgtcatccg
     7501 ctcatgcgtt cacttgttca ctcgggcccg cgaaggcggg tcagctcgga caggccaggg
     7561 ccgaacacca gcttgacgca gtgcgggtgg gcccagccga gctcgagggt gtgccgctgt
     7621 ccctgggtcc aaacctccac ccaccagcgg caccagccga tctgcggcgc ctcgggttca
     7681 ccggcccgcg gcggctcgaa gcggacccac agccacgaca gcttgtgccg cagggtccac
     7741 gggcgggcga acgcctcgac cgcccgcacg agcgcccggt ggtcgacatc attgaagtac
     7801 gccgtcacgt aggtgtcgaa caggatgacc ggcgcgaccg gatggatcgg gaccaccgcg
     7861 cgcaggaagt cctcggtctc gtccggcagc cgggtcttga gcaaccgggc gccttgaccg
     7921 ttgtcgcgga gctcccggcg cagcgccagc gcctcctgca agcgctgcat ccggcgcggc
     7981 tggtcacccc agatgcacgc cgccagctcg gtctccgcgc ccggctcgtc gaggtcgacg
     8041 atacgcaggt cggcgccgac gcgcgcgagg atctccggcg gcttgacgat cgaaggcagg
     8101 gccgggcgcg gcccgtcgca gtcgagctcg aactgccagg tctcgccctg gccgagccgc
     8161 agcggcgcgc cgtcgttcca gcgcaggtcg tagcggcgca ggtccgcgag caggttgagg
     8221 cccgcgctgg cgccgagctc gacgaggtac gcggcgtcga tctgcagcag cgccgccggc
     8281 agcagccaca gcacgccacg cgccgtctcg ttggtctgga tcagccagcg gtcggcctcc
     8341 ctgagcaggt cgtcgccgag gctgtcgagc gcccgcagca gctcgcccgc gaactccgga
     8401 tcttccggcg aggccggctc cgccgtcacc gtcgcgtaat agcgggcgag cccggccacc
     8461 cgcgggtcct cgcgcaatac gtgactgtgc aacgtcgccg cgagcttcaa gatcggctcg
     8521 aggtcgttgt cccacgcgcg cgacgacacg aacttcgcaa agcgctcgcg cagccgcgac
     8581 tgctcgccag gggtccccga agactccccc gcgcccgaaa cttcgccgcg aatacatgcc
     8641 ccgagacagt cgaggatcgc cgccgacaat ggcgagtagc ccctcgcaaa ggtggcctgg
     8701 cgggcgaggc tcgtcaccca ctcctgcgtg tccgctccgc tctgcatggc gctgggggca
     8761 actgtacacc ttgcggtcct ggcacacatc tgaaacgatg cgcgctatcg tgcaggtccc
     8821 cttacgaccc ctcgaggccg cttcctgggc gtcgtccgtc gtgcctggtg cgcgctacga
     8881 tctgtcgagc tccacggggc ccgggacccg cggcagcctc ggcgccgcgc tgccccgcac
     8941 cccggacgag accggcgagg acgagaccgc cgcgagctgg gacgaggccg aggcctcgct
     9001 cgtgcacgcg atcgccagcc gctacggcgt cgacccgatc caggtcgcgc tgacgcccgg
     9061 cgccggtcag gcgatcgttc acgccctgat cgctatgttg cgcccgggcg accacgtcgt
     9121 cgtcgagcgc cccacttacg agccgctgca ccgcgtgccc gagatgctcg gcgccagcgt
     9181 ggcccgcatc gacaggcgca tcgaggacga ctggcagctg ctgcccgagc gcctcgccaa
     9241 ggtgctgacg ccgcgcaccc gcgccgtcgt gctcaccaac ctccacagcc cctcgggcgt
     9301 cgcatgcagc ccgcgaacga tcgccgagat cgccggcctc gccgcgcgcg tcggcgcgac
     9361 cgtgctcgtc gacgaggtcc tgctcgacca cgccttcgac ctcggccccg cggccgcgtg
     9421 ccgacccgcc tgcctggtcg ccgacaacgc gatctgcttc agctcgacca gcaagggcct
     9481 cgggctgccg gagctgcgcg tcggctggct ggtgtcgcgt gacccggacg ccgctcgtgg
     9541 tctgaggatc gccgcggatt acctgcaccg tcggctgccc ccggcgacca tgcgcctcgc
     9601 cgcccacgcg ctgcgcgacc ccgacgactt tcgcatccgc accgcgcggc tcgccggcgc
     9661 cggtcgtcgc gtggtcgagc gctgggtgca gggcgagccg cgcgtcgagt ggatcccgcc
     9721 gcacgccggc ctgcacacgc tcgtgcgcct gcccgtcggc gtctccgacc tcgccttcgc
     9781 ggcccacctg cgcgcccgct acgagaccca ggtgatcccc ggcagcgtgc tcgaggcgcc
     9841 gcagtgcgtg cgcctcggct tcgccgcgcc gagctcgacc ctcgagcagg gcctcgccaa
     9901 cttctcggcc gcgctcgacg acctgctcgg cggctctacc ctggcggggc cgggtttggc
     9961 cccggaccgc ccgttgctcg gggtcgtccc gctccgcgac gacgacgacc cgggcgcagc
    10021 ctgatcagac attgaagcgg aagtgcatga cgtcgccgtc ctgcaccgtg tactccttgc
    10081 cctcgacgcg catcaggccc ttgctcttga ccgcggcctc ggagccgagg gtgacgaggt
    10141 cgttccagtt catgacctcg gcgcggataa agccgcgctc gaagtccgag tggatcttgc
    10201 cggccgcgcc aggggcgagc gtgccgcgct ggatcgtcca cgcgcggacc tcctgcttgc
    10261 cggccgtaaa gtaggtgatc aggtcgagca gcgcgtacgc gcggcggatg agccggctca
    10321 ggcccggctc ctcggcgccg atgccctcga ggaactcgcg gcgctcgtcg ggcgacagcc
    10381 cggagatctc ggcctcgatc gccgccgaga tgacgacgac ctcggtgccc tcgcgggtcg
    10441 cgacctcgac cagccgtgcc accaatggat ccgtcaaccc cgcggccagc tgcgcctcgg
    10501 acacgttggc ggcgtagagc gccggcttgg ccgtcagcag cgcgaggccc tggatcagct
    10561 cgcggtcgaa gtcgtcctgc gggatgttgc ggaccatgat gccgcgaccc atcgcgtcca
    10621 tcagcttctc gcagctgacg agcgccagct tctcggtctt gtcgccgctc ttggacgcct
    10681 tcttggcctt gttgagccgc gcctcgaccg actgcatgtc gcgcagcagc agctccgtat
    10741 tgatgatctc cgcgtcgccg accgggtcga ccttgttgtt gacgtggatg atgttgtcgt
    10801 cctcgaagca gcgcacgacg tgcacgatcg cgtcgacgtt gcggatgtgg gtcaggaact
    10861 ggttcccgag gccctcaccc tggctcgcgc ccttgaccag cccggcgatg tcgacgaact
    10921 cgaccgtggt cggcacgatc ttgttcggct cgacgcactt gaccagcgct tccatccgcg
    10981 ggtccggcac cgagaccatc ccgacgtttg gttcgatcgt gcaaaacggg tagttcgccg
    11041 actgcgcgcc cgcgttggac agcgcgttga acagggtgga cttgcccacg ttggggagcc
    11101 cgacgatgcc gacagccagt gccatggcgg cctcgctacc acgaagcggc ccgccccgcg
    11161 accaagtttt cgcacatcag cgcagggcgc gcggatcaga accggtactc gatgatcagc
    11221 ggcaggatct cgatgccgaa caggaagccc gccttcttgg tcgtcggctg gttcagcggc
    11281 gtgtccttgg agaccgagaa gccgagcacg aagttgacgt cgatgccgat cccgaggtcg
    11341 aggatcggga agaagcgcag gccgccgccc gggcgcacgc tcatcgcgaa ggtcgactca
    11401 cgcgcctgct ggcgcgagcc gccgatgtcc gggtaccacc acttcccgcc gccgatggcg
    11461 ccgcgggcga tcggcaccag gt
//
LOCUS       DASWNJ010000060         1820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_530732, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000060 DASWNJ010000000
VERSION     DASWNJ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530732"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VGB85_01180"
     CDS             complement(<1..320)
                     /locus_tag="VGB85_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0132649.1"
                     /translation="MSAFKDKYGPWAVVCGASEGLGAAFAGALAARGVNLLLLARRAD
                     ALAAVAAPLRARVEVRTAAIDLASPELAAELVRSCGDLDVGLCVYNAAFSQIGPFLEQ
                     SLAD"
     gene            complement(370..969)
                     /locus_tag="VGB85_01185"
     CDS             complement(370..969)
                     /locus_tag="VGB85_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132650.1"
                     /translation="MRNGLVITSVCSLLLAACSSGLSEEDSLVAWGATYAALSQGQAQ
                     AQVAASGTPVVEGEQASFRGLGVYPREAANVNYDWACTGGGTARYAGSAEAVVDDAGA
                     SNVTFDLTAEFDACSVNGITISGDLDYSLDADTTAESASFVVLMKGSMSYEGQVDGSC
                     DWNLTMKIAASSVDGGTGSAEFSGSICGHDAAATLKVNG"
     gene            complement(1025..>1820)
                     /locus_tag="VGB85_01190"
     CDS             complement(1025..>1820)
                     /locus_tag="VGB85_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0132651.1"
                     /translation="RRRWPIVVPAGWRACHTPAAGYLRVAPCLDALRREAEAAGAVIR
                     HHVKVMELVPHASGVRALLESGELVDGDLAVVAAGAYLPALLPGFLPRRLKVVRRVLA
                     WTRPDEAQREPLARLPVWCVFGPDGFHYGFPWSREGVDGFKLACHVTAAGEQADPAED
                     PERVNREVGPGDLAPLDAFLQTYLPRARGPFVASTVCLYTCTPSWDFAIDFLPRDSRV
                     LVAGGFSGHGFKFAPAVGELVAQALLAGALPPALAAFARARHLDTA"
BASE COUNT          299 a          678 c          632 g          211 t
ORIGIN      
        1 ttgtccgcga gcgactgctc gaggaacggt ccgatctgcg aaaacgccgc gttgtagacg
       61 cagagaccaa catcgaggtc accacatgac cggaccagct ccgccgcgag ctcggggctc
      121 gccagatcga tcgccgcggt ccgcacctcg accctcgcgc gcagcggcgc agcgacggcc
      181 gcgagcgcat cggcgcggcg cgccagcaac aacaggttga caccccgcgc cgcgagggcc
      241 ccggcgaacg ccgccccgag cccctccgag gcgccgcaga cgaccgccca cggcccgtac
      301 ttgtctttga acgccgacac gcgcgcagct tacacgagcg cgcgcgtgcg gcgatagacg
      361 gggagctgct cagccgttga ccttgagggt ggcggccgcg tcgtggccgc agatcgaccc
      421 ggagaactcg gcgctgccgg tgccgccgtc gacgctggac gcggcgatct tcatcgtcag
      481 gttccagtcg cacgagccgt cgacctggcc ctcgtagctc atcgagccct tcatcagcac
      541 gacgaaggac gcggactcgg ccgtggtgtc ggcgtcgagc gagtagtcga ggtcgccgga
      601 gatcgtgatg ccgttgaccg agcaggcgtc gaactcggcg gtgaggtcga aggtcacgtt
      661 gctcgcgccg gcgtcgtcga ccaccgcctc ggccgagccg gcgtagcgcg ccgtgccgcc
      721 gcccgtgcag gcccagtcat aattcacgtt cgcggcttca cgcgggtaca cgccgaggcc
      781 gcgaaagctg gcctgctcgc cctcgacgac cggcgtgccg ctggcggcca cctgcgcctg
      841 cgcctggccc tgcgagagcg ccgcgtaggt ggcaccccac gcgaccagcg agtcctcctc
      901 gctgaggccg ctcgagcacg cggcgagaag aagcgaacat accgaggtga tgacgagacc
      961 gttgcgcatc gctgcaaggt accgcaagac gccacccggc gggtgacccg ccgtgacgcg
     1021 agatctacgc tgtgtccagg tgtcgcgcgc gtgcgaacgc cgcgagcgcg gggggcagcg
     1081 cgcctgcgag cagggcctgc gcgaccagct caccgaccgc gggcgcgaac ttgaagccgt
     1141 gccccgagaa cccgccagcg acgagcaccc ggctgtcgcg cggcaagaag tcgatcgcaa
     1201 agtcccacga gggcgtgcag gtatacagac acaccgtcga cgccacgaac gggccacgcg
     1261 cccgcgggag ataggtctgc aagaacgcat cgagcggcgc gaggtcgccg ggcccgacct
     1321 cgcggttcac ccgctccggg tcctccgccg ggtcggcctg ctcaccggcc gcggtcacgt
     1381 ggcacgcgag cttgaagccg tcgacgccct cgcgtgacca tgggaaccca taatgaaagc
     1441 cgtcgggccc gaacacgcac cacaccggca ggcgcgcgag cggctcgcgc tgcgcctcgt
     1501 ccggccgcgt ccacgcgagc acgcggcgca cgaccttgag ccgtcgcggc aagaagcccg
     1561 gcagcagggc cggcaggtac gcccccgcgg cgaccaccgc gaggtcaccg tcgaccagct
     1621 cgcccgactc gagcagcgcc cgcacgcccg aggcgtgggg gacgagctcc atcaccttca
     1681 cgtgatggcg gatcaccgcg ccggccgcct cggcttcgcg tcgcaatgca tcgaggcacg
     1741 gcgccacgcg cagatagccg gccgcggggg tgtgacaggc gcgccagccc gcgggcacca
     1801 cgatcggcca gcgccgccgg
//
LOCUS       DASWNJ010000061         1845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_177712, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000061 DASWNJ010000000
VERSION     DASWNJ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177712"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VGB85_01195"
     CDS             <1..366
                     /locus_tag="VGB85_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132652.1"
                     /translation="FSGAGGRSVLAPGTRPLVRVEPVKTTARVALLSASSGAQISGPT
                     SDGKGGLFSKYLMDALGSGQADINGDAQISLKELDEWIRPRVAREARKANREQTPNVQ
                     VGKKLGAPDDFIVAWGYAR"
     gene            405..1448
                     /locus_tag="VGB85_01200"
     CDS             405..1448
                     /locus_tag="VGB85_01200"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HEY0132653.1"
                     /translation="MSAACTKQGFLAPPGVDQAITAKVGSFAPDMHFLIEPTGDAEDL
                     LGRAVHVTSAGGWTIADERAPGCDVRVKRSAADYEKSYRMALGDLTALSGGYGELLKL
                     EARYGRSVEAEMKIHNLETLTADIEGDCGEVIVKSVRIGTGERKLLRKAEGVAKVNVG
                     KGPIGVSAGREAATDAADEIRWSTPQAYAFTYEGGVRRKVFELEAQIPGTLRNGETFE
                     LVFTGSEDAYLVIYYLEENGEGTVLFPDAGVPVPMFAAGETLSLPPAGQKKLQAQLRD
                     PTIPERGTVVVYAFAERGDFDRFRPAALTDSTDALAYAAELTRVLSSVPISRWDRHTT
                     TIEILPNAETSKP"
     gene            1445..>1845
                     /locus_tag="VGB85_01205"
     CDS             1445..>1845
                     /locus_tag="VGB85_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132654.1"
                     /translation="MTRPLATLLRPVVAALCSLTLALPASARAAEPAPAPIEGPLTDS
                     LSPKAAEHVARAYEEHKAGAYLNAESAFKRAAFFAPRWRPLHYNLGIVAEAQGHLGTA
                     IREYRTFRPYATDDEGMVVDQRIVELDARRRR"
BASE COUNT          283 a          735 c          572 g          255 t
ORIGIN      
        1 ttctcgggcg cgggcggccg ctcggtgctc gcccccggca cccgcccgct ggtgcgcgtc
       61 gagccggtca agaccaccgc gcgcgtcgcc ctgctgtcgg cctcctcggg cgcccagatc
      121 tcgggcccga cctccgatgg caagggcggc ctgttctcaa aatacctgat ggacgcgctc
      181 ggctcgggcc aggccgacat caacggcgac gcccagatct cgctcaagga gctcgacgag
      241 tggatccgcc cccgcgtcgc ccgagaggcc cgcaaggcca accgcgagca gaccccgaac
      301 gtccaggtcg gcaagaagct cggcgcaccc gatgacttca tcgtcgcgtg ggggtacgcg
      361 cggtgatgcg tgcttccctc gtcctcgcat ctgtcctttg ggtcatgtcc gccgcgtgca
      421 cgaagcaggg cttcctcgcc ccgccggggg tcgaccaggc gatcaccgcc aaggtcggca
      481 gcttcgcccc cgacatgcac tttttgatcg agccgaccgg cgacgccgag gacctgctcg
      541 gccgcgccgt ccacgtcacc agcgcgggcg gctggacgat cgccgacgag cgcgcgcctg
      601 gctgtgacgt ccgcgtcaag cggtccgccg ccgattatga aaaatcgtac cgcatggccc
      661 tcggcgacct gaccgcgctg tccggcggct acggcgagtt attaaaactc gaggcccgct
      721 acggccgctc ggtcgaggcc gagatgaaga tccacaacct cgagaccctc accgccgaca
      781 tcgagggcga ctgcggcgag gtgatcgtca agtccgtgcg gatcgggacc ggcgagcgca
      841 agctgctgcg caaggccgag ggcgtcgcca aggtcaacgt cggcaagggc ccgatcggcg
      901 tgtccgccgg ccgcgaggcg gccaccgacg cggccgacga gatccgctgg tcgacccccc
      961 aggcctacgc cttcacctac gagggcggcg tccgccgcaa ggtcttcgag ctcgaggccc
     1021 agatccccgg caccctgcgc aacggcgaga ccttcgagct ggtcttcacc ggctccgagg
     1081 acgcctacct cgtaatctac tacctcgagg agaacggcga gggcaccgtg ctgttccccg
     1141 acgccggcgt accggtgccg atgttcgccg ccggcgagac cctctcgctg ccccccgccg
     1201 gccagaagaa gctgcaagcc cagctgcgtg atcccacgat ccccgagcgc ggcacggtcg
     1261 tcgtctacgc cttcgccgag cgcggcgact tcgaccgctt ccgtcccgcc gccttgaccg
     1321 acagcaccga cgcgctcgcc tacgccgccg agctgacccg cgtcctgtcc tccgtcccga
     1381 tcagtcgctg ggaccgccac accacgacga tcgagatcct cccgaacgcc gagacttcca
     1441 agccatgaca cgccccctcg cgaccctgct gcgccccgtc gtcgccgcgc tttgcagcct
     1501 caccctcgcg ctcccggcca gcgcccgcgc agccgagccc gcgcccgcgc cgatcgaggg
     1561 cccgctcacc gacagcttga gccccaaggc cgccgagcac gtcgccaggg cctacgagga
     1621 gcacaaggcc ggcgcctacc tcaatgccga gtccgccttc aaacgcgccg ccttcttcgc
     1681 cccgcgctgg cgccccctgc actacaacct cggcatcgtc gccgaggccc agggccacct
     1741 cggcaccgcg atccgcgagt accggacctt tcgcccctac gccaccgacg acgagggcat
     1801 ggtcgtcgat cagcgcatcg tcgagctcga cgcccgtcgt cgccg
//
LOCUS       DASWNJ010000062         2688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_142580, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000062 DASWNJ010000000
VERSION     DASWNJ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142580"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VGB85_01210"
     CDS             <1..241
                     /locus_tag="VGB85_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132655.1"
                     /translation="VRVPYFLAAPEMVAASDLILTTAELVAQHFAKLYPLQVLAPPID
                     LPPFGIDAVWHERFDQDPAHRWLRGQVAAVMRGFA"
     gene            complement(245..1366)
                     /locus_tag="VGB85_01215"
     CDS             complement(245..1366)
                     /locus_tag="VGB85_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132656.1"
                     /translation="MTGCGDDGGASTTSASTGDAGTTTDTPTTDEPTTATTGTDEPTT
                     TTTTATTGEPDPTTTGGDTSGSDTSTGDPAAEPEIVHEFDVAMFQLPEGLDVYEGTAY
                     VGLVTGQVFTVDPEDGTTAMFGGLVGLPQDNTAIMTGLIVGPDGAVYVALDVFAPGDF
                     VSGIYKIPAGGGLGVPFASDPAMAFPNGFAFDAKGDLLVTDSFTATIFHVAIADGTVT
                     PWVQDPLLAPNPDTCKLDSAFHLGANGITRAGDRVFVTNSDQASILEIPVDAMGKAGA
                     PKLLLGPDCEALSGADGLIVEPNTDNLLVPVNYKQRIIRVDATQQITVLAEGGLLQSP
                     ATLVMAPAGDAVLIANAAFETATTDPANAHPALLSLGLQ"
     gene            complement(1552..2421)
                     /locus_tag="VGB85_01220"
     CDS             complement(1552..2421)
                     /locus_tag="VGB85_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132657.1"
                     /translation="MPSRTRFAWPVPGMVQVDVTSTQLRMSVRWKFTLTITAAPDGVR
                     RIVRWTNPTILPGQQFDESHDQVLRVVVMQHALMAAAPELVVSPTGALVDIRIEPQEV
                     LRVIFPAVVGRWRPQLVEAMTEAVERPEFRAIQADRAAALWNSWVGAWIELPTTPGPL
                     ERVDGRVEGLGGIAMRDAFGARQGPTTLPGHILLSRRTLTDMHAQSMHHFVQATMGGD
                     LSGVSDGSPPATHEDAVEAVTDPGSLRPHSVRVTSSFYIMVPDPYPFESVYTFHWPAA
                     QGADARDARGSRQ"
BASE COUNT          400 a          909 c          956 g          423 t
ORIGIN      
        1 cgtgcgcgtg ccctacttcc tcgcggcgcc ggagatggtc gccgcctccg acctgatcct
       61 gacgaccgcc gagctcgtcg cgcagcattt tgcgaagctc tatccgttgc aggtcctcgc
      121 gccgccgatc gatctcccgc ccttcggcat cgacgccgtg tggcacgagc gcttcgatca
      181 ggaccccgcg catcgctggc tgcgcgggca ggtcgccgcc gtcatgcgcg gcttcgcgtg
      241 aagctcactg cagccccagc gagagcagcg ccgggtgggc gttggccggg tccgtcgtcg
      301 ccgtctcgaa ggccgcgttg gcgatcagca ccgcgtcgcc ggccggggcc atcaccaggg
      361 tcgcgggcga ctgcaacagg ccgccctccg cgagcaccgt gatctgctgc gtcgcgtcga
      421 cgcggatgat ccgctgcttg tagttgaccg gcaccagcag gttgtcggtg ttcggctcga
      481 cgatcagccc gtccgcgccc gacagcgcct cgcagtcggg cccgagcagc agcttcggcg
      541 cgccggcctt gcccatcgcg tcgaccggga tctcgaggat cgaggcctgg tccgagttgg
      601 tgacgaacac gcggtcaccc gcgcgcgtga tgccgttggc gccgaggtgg aacgcgctgt
      661 cgagcttgca ggtgtcgggg ttcggggcga gcagcgggtc ctgcacccac ggcgtcaccg
      721 tgccgtcggc gatcgccacg tggaagatcg tcgcggtgaa cgagtcggtg accagcaggt
      781 cgcccttggc gtcgaaggcg aagccgttgg ggaaggccat cgccgggtcg ctggcgaacg
      841 gcacgccgag cccgccgcct gcgggaattt tatagatgcc gctgacgaag tcgcccggcg
      901 cgaacacgtc gagcgcgacg tagaccgcgc cgtcggggcc gacgatcagg ccggtcatga
      961 tcgcggtgtt gtcctgcggc agaccgacga gcccgccgaa catcgccgtc gtgccgtcct
     1021 ccgggtcgac cgtgaacacc tggccggtga cgaggccgac gtacgcggtc ccctcgtaca
     1081 cgtcgaggcc ctcgggcagc tggaacatcg ccacgtcgaa ctcgtgcacg atctccggct
     1141 ccgccgcggg gtcgcccgta ctggtgtcgc tgccgctggt gtcgcccccg gtggtcgtcg
     1201 ggtccggctc gccggtcgtc gcagtggtgg tcgtcgtggt cggctcgtcc gtgcccgtgg
     1261 tcgcggtggt cggctcgtcc gtggtcgggg tgtccgtcgt ggtcccggcg tcgccggtgc
     1321 tcgccgaggt cgtgctcgcg ccgccgtcgt cgccgcagcc ggtcatgagc gcgagtaata
     1381 gagcggaggc acgcagattc gaagtatgca tgcccctgct ctaccgccgt cgccgcgggt
     1441 cgaccaatgg cttgttctga tacggacatg cgcggcgctc atgatgaaca gggtgatgaa
     1501 gcccgcgcag ccgagcccga acataccgag cgccattgct gatgagcaat gttattggcg
     1561 cgagccccga gcgtcgcgcg catcggcgcc ctgggcggcc ggccaatgaa acgtatacac
     1621 gctctcgaaa gggtatggat ccggtaccat gatatagaag ctcgacgtca cccggaccga
     1681 gtgcgggcga agtgaccccg ggtcggtgac ggcctcgacc gcatcttcgt gcgtggccgg
     1741 cggcgagcca tcgctgacgc cggacagatc gccgcccatg gtcgcctgga cgaagtgatg
     1801 catgctctgc gcgtgcatgt cggtaagagt cctgcgggag agtaggatgt ggccggggag
     1861 cgtggtgggg ccctggcgcg cgccgaacgc atcgcgcatg gcgatcccgc ccaatccctc
     1921 cacccgccca tcgaccctct cgaggggacc cggcgtcgtc ggcagctcga tccaggcgcc
     1981 gacccaggaa ttccacaggg ccgcggcccg gtcggcctgg atcgcacgaa actccggccg
     2041 ctccacggcc tcggtcatcg cctcgacgag ctgcggccgc cagcgaccga cgacagccgg
     2101 gaagatcacg cgcaggacct cctgtggctc aatccttatg tcgacgagcg cgcccgtggg
     2161 actgacgacg agctccgggg ccgcggccat cagcgcgtgt tgcataacca cgacccgcag
     2221 cacctggtcg tggctctcgt cgaactgctg gcccggaagg atcgtcgggt tggtccagcg
     2281 gacgatccgg cggacaccgt ccggggcggc tgtgatcgtg agcgtgaatt tccagcgcac
     2341 cgacatcctg agctgcgtgg aggtcacgtc gacctggacc atgccgggta caggccaggc
     2401 gaagcgcgtc cgcgatggca tcggagcagc ggcgaccggc gccacagcgg gcggcggcgg
     2461 tcgggattcg acggcgtggt ggcagccagc agcggagccg ctcagcagca catgcgatat
     2521 gaacagggaa tgaagtcggt tttgcatggc acgcatcggt gacgctcagt cgccgtaccc
     2581 ggcatcaaca gcgccactgg tacgacgacc agaggcatcg ccgtggcggc agaggctgga
     2641 ccaacgggcg gggacggctc gacatggcct ggtagcgtcg gccggccg
//
LOCUS       DASWNJ010000063         3085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_530753, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000063 DASWNJ010000000
VERSION     DASWNJ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530753"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1360)
                     /locus_tag="VGB85_01225"
     CDS             complement(<1..1360)
                     /locus_tag="VGB85_01225"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0132658.1"
                     /translation="MRESIVFGLSCLVLACTPTPTPDQGVAVTVSVPRPERFEIEAID
                     AYVRAQVEKKGFVGLSLAVVRNGEVVLARGYGKRSLADGAAVDEDTVFAIGSVTKQFT
                     CASALLLAEDGKLRLDDKVSTYRPELARAADITLYDLLSNVSGYPDYYPLDFVDERMA
                     TPVDPDALLRQYAGGTLDFEPGSRWSYSNTGFVLAGRVIEAVSGQSFDAFLKERIFGP
                     VGMQRSFFTVTADTPGLATGYTTFALGEPRPAAPEAAGWMHAAGAIYATATDLARWDL
                     ALIDGKLLSADSWHRMTTPRTLTTGELTDYGCGVAIARRGGETVVQHSGAVSGFLAWN
                     AMIPRTRSAVIMLANADFVDGGGLHGELLSLLIEADRPRPTVPGPGPRETALALLHQM
                     QAGEIDRAALGEPFSKFLDEQRLQEAAPRLRALGEPTAVVVERLSERGGMEVASLRFH
                     FAG"
     gene            1507..2211
                     /locus_tag="VGB85_01230"
     CDS             1507..2211
                     /locus_tag="VGB85_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEY0132659.1"
                     /translation="MQALRIDVWSDIACPWCHVGKRRLEAALEQFPHRDAVEVVWHSF
                     ELDPSAPRTSEAAPYAGRLARKYGTSVADAQQMIDRMTETAARDGLDFHFEKIRPGNT
                     FDAHRLLHLAHTRGLQDALKERLFLAYLGEGEAIGEPEVLARVAVEAGLELDEVRRVL
                     DGDAFAAAVRADEEQARSLGIRGVPFFVFDGRHAVSGAHPSDTLLAALTTAWEAAKIR
                     APELAEGASCGPDGCA"
     gene            2372..>3085
                     /locus_tag="VGB85_01235"
     CDS             2372..>3085
                     /locus_tag="VGB85_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132660.1"
                     /translation="MFSLLMPACYAGSGGDGEGSSTSTTQSASTSGDATTAPLTTGAT
                     DTDDLTTTPTTDALTSSTTDAPTSSTTDALTSSTTSSTTGEPGTTGEPDTSTGSTDTG
                     SDTTDPGTTSGTSTGGGFDPAHIPEIPDDGMPSSAHLKKVPLGTSDASAGYYEYTPPG
                     YGGGELYPLLVFYHGIGENGNGDSELGKVLANGPPKLINSDNWAKDRPFVVLSPQHAG
                     GGCPSASEIHDFFTFAMSHY"
BASE COUNT          487 a         1228 c          956 g          414 t
ORIGIN      
        1 tgccggcgaa gtgaaagcgc agcgaggcca cctccatccc gccgcgctcg gagagccgct
       61 cgaccaccac cgcggttggt tcaccgagcg ccctcagccg cggcgctgcc tcctgcagcc
      121 gctgctcgtc gaggaacttt gaaaagggct ccccgagcgc agcgcggtcg atctccccgg
      181 cctgcatctg atgcagcagc gcaagcgccg tctcacgcgg ccccggtccg ggcaccgtcg
      241 gtcgcgggcg gtcggcctcg atcaacagcg acagcagctc gccgtgcagc ccaccaccat
      301 ccacgaagtc ggcgttggcg agcatgatca ccgccgagcg cgtgcggggg atcatcgcgt
      361 tccacgccaa aaacccgctg accgccccgc tgtgctgcac caccgtctcg ccgccgcgtc
      421 gcgcgatcgc cacgccgcag ccgtagtcgg tcagctcgcc ggtcgtcagc gtgcgcggcg
      481 tcgtcatgcg gtgccacgag tcggcgctga gcagcttccc atcgatgagc gccaggtccc
      541 agcgcgcgag gtccgtcgcc gtcgcgtaga tcgcaccggc cgcgtgcatc caccccgcgg
      601 cctcgggcgc cgccggacgc ggctcaccga gcgcgaaggt cgtgtagccg gtcgccagcc
      661 ccggcgtgtc ggccgtgacc gtaaagaacg accgctgcat cccgaccggc ccgaagatcc
      721 gctcttttaa aaatgcatcg aagctttggc cgctgaccgc ctcgatcacc cgccccgcga
      781 gcacgaagcc ggtgttgctg tacgaccagc gcgagcccgg ctcgaagtcg agggtcccgc
      841 ccgcgtactg ccgcagcagc gcgtccgggt cgaccggcgt cgccatgcgc tcgtcgacga
      901 agtcgagcgg ataataatcc gggtatccgg acacattact caacaggtca tatagcgtga
      961 tgtcggccgc acgcgccagc tccggccgat atgtggacac tttgtcgtcg agccgtagct
     1021 tgccgtcctc ggccagcaac agcgcgctcg cgcaggtgaa ctgcttggtc accgagccga
     1081 tcgcgaacac cgtgtcctcg tcgaccgccg cgccgtcggc gagcgagcgc ttgccgtagc
     1141 cacgcgcgag caccacctcg ccgttgcgaa cgaccgccag cgacagcccg acgaagccct
     1201 tcttctcgac ctgcgcgcgc acgtaggcgt cgatcgcctc aatttcaaac cgctccggac
     1261 gcggcacgct caccgtcacc gcgaccccct gatccggcgt cggggtgggc gtgcaggcga
     1321 gcacgaggca cgacaggccg aacacgatgc tttcgcgcat cccgcgacgg tacgcccgcc
     1381 tgccgcacca cgacaagccg gaccgcccga agaacgacct gacagagcac ccggcgcgcc
     1441 gggcgtgcgc tcgcccacct gctgccgccc tcgcgcgccc ccgggcccgg acgcatgctg
     1501 atgcgcatgc aagctctgcg catcgacgtg tggtccgaca tcgcgtgccc ctggtgccac
     1561 gtcggcaagc gccggctcga ggcggcgctc gagcagttcc cgcaccgcga cgcggtcgag
     1621 gtggtctggc actccttcga gctcgacccc tccgctccgc gcacctcgga ggcggccccg
     1681 tacgccgggc gtctcgcccg caagtacggc accagcgtcg ccgacgcgca gcagatgatc
     1741 gaccgcatga ccgagaccgc cgccagggac ggcctcgact tccacttcga gaagatccgc
     1801 cccggcaaca ccttcgacgc ccaccgcctg ctccacctcg cccacacccg cggactgcag
     1861 gacgccctca aggagcgcct gttcctcgcc tacctcggcg agggcgaggc gatcggtgag
     1921 cccgaggtgc tcgcgcgcgt cgccgtcgag gccggcctcg agctcgacga ggtccgccgc
     1981 gtgctcgatg gcgacgcctt cgccgcggcc gtgcgcgccg acgaggagca ggcgcgcagc
     2041 ctcggcatcc gcggcgtgcc cttcttcgtg ttcgacggcc gccacgccgt ctccggcgcc
     2101 cacccctccg acaccttgct cgccgcgctg acgaccgcct gggaggccgc gaagatccgc
     2161 gcgcccgagc tcgccgaggg agcctcgtgc ggccccgacg gctgcgcgtg agcgacttcg
     2221 tagcagtgca cggcccccgg ccggggcccc ccgtcacgcc tcacggtcgc attgcgagcg
     2281 cccgcgggcg ctcgccttga cgtgctttgt cggcggttga tagaccgcga tcatggttgt
     2341 tcttcgtcgt cgccctgcat cgatgctcgc cttgttttcg ctcctcatgc ccgcctgcta
     2401 cgcgggctcc ggcggagacg gcgagggttc gagcacgagc acgacccaga gcgccagcac
     2461 cagtggcgac gcgaccaccg cacccctcac caccggcgcc accgataccg acgacctgac
     2521 gaccacgccg accaccgacg cgctcaccag ctcgaccacc gacgcgccga ccagctctac
     2581 caccgacgcg ctcaccagct cgaccaccag ctcgaccacc ggcgagcccg gtaccacggg
     2641 cgaacccgac acgagcaccg gcagcaccga caccggcagc gacaccaccg accccggcac
     2701 caccagcggc acgagcaccg gtggcggctt cgacccggca cacatccccg agatccccga
     2761 cgatggcatg ccgtcctcgg cgcacctgaa aaaagtcccg ctcggcacct ccgacgcgtc
     2821 cgccggctac tacgagtaca ccccgcccgg ctacggcggc ggcgagctgt acccgctgct
     2881 cgtcttttat cacggcatcg gcgagaacgg gaacggcgac agcgagctcg gcaaggtgct
     2941 cgccaacggc ccgcccaagc tgatcaactc cgacaactgg gccaaggacc gccccttcgt
     3001 ggtgctctcg ccgcagcacg ccggcggcgg ctgcccgagc gccagcgaga tccacgactt
     3061 cttcaccttc gcgatgtcgc actac
//
LOCUS       DASWNJ010000064         2346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_601853, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000064 DASWNJ010000000
VERSION     DASWNJ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601853"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VGB85_01240"
     CDS             <1..838
                     /locus_tag="VGB85_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132661.1"
                     /translation="SDLMEEVVTIAAKFAEEGAMPLVQRFAQHPAPEVRTRLANAMYF
                     DADAALPGRRELILALCRDADVGVRAAAFGACASLVDDREVVRWVAACAAQDPSPEVQ
                     RACLSAISLGSHYGSNDLLLDTYLQHAQQNPSADVREGVAQSLHWLPADPRLTRIVAA
                     LFADRSSEVRRAIAWQSNNMSEHPELRDLYVQAATSDPDESVRADALRGLHHFFPLTD
                     AVAFLRQRLAQDRNEATYWAALNAAESHLPATEARALIQDIARGPFSSVGERAREVLA
                     GN"
     gene            985..2136
                     /locus_tag="VGB85_01245"
     CDS             985..2136
                     /locus_tag="VGB85_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132662.1"
                     /translation="MSAARAILVASMLALVGVPLLVRLAVYGDMPATWGRFPPPQSAG
                     VPGFNWLVFLAGVALALGIVAWFAAPRWFGFRPVPAAQPPARAGFPAWFWPGVALCLG
                     AWAVAWGLVPAGPLARFAFVPQWWGFILALDGVVYRRNGGVSLLSRRRGGILAIAGVS
                     CVSWYFFEYLNYFVGSNWYYPHDDLFSQTGYLLWFGLAYTTVLPSIFVFYTLLTTFPR
                     LRARFAAGPRVALARRGWERVLVLGLVTLALLIVWPAPLFPVLWLSPLMVVAGALMLA
                     RRWTPFTPLERGDWAPLALIALACALNYLVGELWNYYSTAENPNFWKYDVPYVNDPKL
                     FEMPVLGFFGYLPFGVLCWTWWLHHAALLRVDPSIDVVPGLGPTTLADP"
     gene            2165..>2346
                     /locus_tag="VGB85_01250"
     CDS             2165..>2346
                     /locus_tag="VGB85_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0132663.1"
                     /translation="MAELRDKRCLIAGGTGGVGEGIVRAFLNAGATVFVPSRSAAKLD
                     ALAGSIAAKPRDRLVTL"
BASE COUNT          267 a          882 c          801 g          396 t
ORIGIN      
        1 ctcggacctg atggaggagg tcgtgacgat cgccgccaag ttcgccgagg agggcgcgat
       61 gccgctggtc cagcgcttcg cccagcaccc ggcgccggag gtgcgcacgc gcctggcgaa
      121 cgcgatgtac ttcgacgccg acgccgccct gcccggccgc cgcgagctga tcctcgcgct
      181 gtgtcgggac gccgacgtcg gcgtgcgcgc ggccgccttc ggcgcgtgtg ccagcctcgt
      241 cgacgaccgc gaggtcgtcc gctgggtcgc ggcctgtgcc gcgcaggacc cctcgcccga
      301 ggtccagcgc gcgtgcctct cggcgatctc gctcggcagt cactacggca gcaacgacct
      361 gctgctcgac acctatctcc agcacgcgca gcagaacccg agcgccgatg tccgcgaggg
      421 cgtcgcgcag tcgctacact ggttgccggc cgacccgcgg ctgacccgca tcgtcgccgc
      481 gctgttcgcc gaccgctcct ccgaggtgcg gcgcgcgatc gcctggcaga gcaacaacat
      541 gagcgagcac cccgagctgc gcgacctgta cgtgcaggcc gccacctcgg accccgacga
      601 gtcggtccgc gcggacgcgc tgcgcggcct ccaccacttc ttcccgctca ccgacgccgt
      661 cgccttcctg cgccagcgcc tcgcccagga ccgcaacgag gcgacctact gggccgccct
      721 caacgcggcc gagagccacc tgcccgccac cgaggcccgc gcgctgatcc aggacatcgc
      781 ccgcggcccg ttctcgtcgg tgggcgagcg cgcgcgggag gtgctcgccg gcaactgatc
      841 gactttcgtc ggtgatcagc gacgggcggg gcgtgaccgc tggcggcgaa atgtgcgatc
      901 ctgtccgcgc atgtcgacga gcgatccagg tcccgtcacc attctcccgc cggcccggtc
      961 ctcatcgcgc cgccggaggt cgtcgtgagc gccgcgcggg cgatcctcgt cgcctcgatg
     1021 ctggcgctcg tcggcgtgcc gctgctggtc cggctcgccg tgtacggcga catgcccgcg
     1081 acgtggggcc ggttcccgcc gccgcagagc gccggggtcc cgggctttaa ctggctggtg
     1141 ttcctcgccg gcgtcgcgct cgcgctcggc atcgtcgcgt ggttcgccgc gccgcgatgg
     1201 ttcggctttc gtccggtccc cgccgcgcag ccgccggccc gcgccggctt ccccgcgtgg
     1261 ttctggcccg gcgtcgcgct gtgcctcggc gcgtgggcgg tcgcgtgggg cctggtcccg
     1321 gccggcccgc tggcgcgctt cgccttcgtg ccgcagtggt ggggctttat cctcgcgctc
     1381 gacggcgtcg tctaccggcg aaatggcggc gtctcgctgc tgtcgcgccg gcgtggcggc
     1441 atcctggcga tcgccggggt gtcgtgcgta tcatggtact tcttcgagta cctcaattac
     1501 ttcgtcgggt cgaactggta ctacccgcac gatgacctgt tctcgcagac cggctatctg
     1561 ctgtggttcg ggctcgccta caccaccgtg ctgccgtcga tcttcgtgtt ctacaccctg
     1621 ctgacgacct tcccgcgcct gcgcgcgcgc ttcgccgccg gaccgcgcgt cgcgctggcg
     1681 cgacgcggct gggagcgggt gctcgtgctc gggctcgtga cgctcgcgct gctgatcgtc
     1741 tggccggcgc cgctgttccc ggtgttgtgg ctcagcccgc tgatggtcgt cgccggggcg
     1801 ctgatgctgg cgcgccgctg gaccccgttc accccgctcg agcgcggcga ctgggccccc
     1861 ctcgcgctga tcgccctcgc ctgcgcgctc aactacctcg tcggcgagct gtggaactat
     1921 tacagcaccg ccgagaaccc gaacttttgg aagtacgacg taccctacgt caacgacccc
     1981 aagctgttcg agatgccagt gctcggcttc ttcggctacc tcccgttcgg ggtgctgtgc
     2041 tggacgtggt ggctgcacca cgccgcgctg ctgcgcgtcg acccgtcgat cgacgtcgtg
     2101 cccggcctcg gcccgacgac actcgcggac ccctgaaacc gcacgacgac cacggagcat
     2161 gatcatggcc gagctgcgag acaaacgatg tttgatcgcc ggcgggaccg ggggcgtcgg
     2221 tgagggcatc gtccgcgcct ttttaaacgc cggcgcgacc gtcttcgtgc cgtcgcgcag
     2281 cgcggcgaag ctcgacgcgc tcgcggggtc gatcgcggcg aagccgcgag accgactggt
     2341 gacgct
//
LOCUS       DASWNJ010000065         3703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_672192, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000065 DASWNJ010000000
VERSION     DASWNJ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672192"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1474)
                     /locus_tag="VGB85_01255"
     CDS             complement(<1..1474)
                     /locus_tag="VGB85_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0132664.1"
                     /translation="MSGEEDTQRTGTGKPSALLEALLERSDLPLDSGEVDDDTNDGEL
                     EVRAETSDRDPGESSQFLEFASLKRFTIRRRIGAGGMGVVYEAIDRERGETVAIKTLL
                     NVSPGALYRFKREFRLLADVTHPNLIALHELFFEGEQIFFSMEYVDGLGFVEYVRGSD
                     SDDATEDPEVRHERLRAALRQVAAGLGVIHGAGMLHRDIKPSNVLLTREGRLVILDFG
                     LVSESLNETLRSTAAGAMIGTPGYLSPEQAAGHPASAASDWYAVGVMMFVALTGELPF
                     RGKGLKVLIAKQEKEAPSPGTLVPGVPPDLEALCVDLLQRDPTKRPGTAEVLRRVGSG
                     PSGIRPRDAVRRDTGDLFLGREAHLKALQDAFAATHRGRAVAVHVGGRSGMGKSALVR
                     EFLARLQRSDRAVTLVGRCYERESVSFKALDSLVDALCQHLMRLPDARAAELMPRDIL
                     ALARVFPVLQRVKAVSQSPPRVGESPDPRELRRRAFAALKE"
     gene            1687..2898
                     /locus_tag="VGB85_01260"
     CDS             1687..2898
                     /locus_tag="VGB85_01260"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY0132665.1"
                     /translation="MTSDARTPVIVAAVRTPIGRFRGALASVRPDDLAALVIRSLAEH
                     HSAAMEQLADVYLGCANQAGEDNRNVARMATLLAGLPVEVPGVTVNRLCGSGMEAVLQ
                     AAKSIMVGEGEVYIAGGVESMTRAPFVMPKATEAFAREAQFFDTALGWRMVNPAMERL
                     FGIEPLGITAENVAERYHISREDQDAWALGSHQRAAAAQDEGWCDNEIIPVMIPGAKG
                     QEPVRFAADEGIRRESSLESLAKLKPAFKPGGSVTAGNSSSLNDGAAALLITSLAKAH
                     NLGLRPMARIASWGHAGVAPGVMGIGPVPASRCALKRAGLTSADIGIAEFNEAFASQT
                     LACLRQLDLDPAKVNPGGGAIALGHPLGCSGARITATLAHSMARTRTRHGLATMCIGV
                     GQGIALVLERT"
     gene            2972..3661
                     /locus_tag="VGB85_01265"
     CDS             2972..3661
                     /locus_tag="VGB85_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132666.1"
                     /translation="MRFLARSTLASTLLFACGDNGDMQITQTNPTSLDTGGSSGTSGM
                     SGASMGDTTTTGQTSTPPTTDPTTDELTTEPLTTGPTTAPTTDASTSEATTEATTDGS
                     SGDSGGVEDLMKVHGHACGTDDDCKALLGPDGICQKDILGVYSLPGGYCTTYCQLPDQ
                     QTTYQLDSRFCTLGADCVGLMGYFEACAHVCTDTAQCPREGYECRQMPQISNPDDPKF
                     CLMTEDNMIPP"
BASE COUNT          577 a         1400 c         1228 g          498 t
ORIGIN      
        1 gctccttgag ggccgcgaag gcgcggcgtc gcagctcgcg cgggtcgggg ctctcgccga
       61 cccgcggcgg cgactgggac accgccttga cgcgctgcaa caccgggaac acccgcgcga
      121 gcgccaggat gtcgcggggc atcaattcgg ccgcccgcgc gtcgggcagg cgcatcaaat
      181 gttggcacag ggcgtcgacg aggctgtcga gcgccttgaa cgacaccgac tcgcgctcgt
      241 agcagcgacc gaccagcgtc accgcgcggt cgctgcgttg gagtcgggcc aggaactcgc
      301 gcaccagcgc cgacttgccc atgcccgatc gcccgccgac gtgcaccgcg accgcgcgac
      361 cgcggtgcgt cgccgcgaac gcgtcctgca gcgccttgag gtgggcctcg cggccgagga
      421 acaggtcgcc ggtgtcgcgg cgcacggcgt cccgcgggcg gatcccgctc ggcccggagc
      481 cgacgcggcg cagcacctcc gcggtgcccg gtcgcttcgt cgggtcgcgc tgcagcaggt
      541 cgacacacag cgcctcgagg tccggcggga ccccgggcac cagcgtgccg ggcgacggcg
      601 cctctttctc ctgcttggcg atcagcacct tgaggccctt gccgcgaaac ggcagctcgc
      661 cggtcagcgc gacgaacatc atcacgccga ccgcgtacca gtcgctcgcc gccgaggccg
      721 ggtgacccgc ggcctgctcc ggcgagagat agccgggcgt gccgatcatc gcgccggccg
      781 cggtcgatcg cagcgtctcg ttgagggact ccgagacgag cccgaagtcg aggatcacca
      841 gccggccctc gcgcgtcagc agcacgttgg acggcttgat gtcgcgatgc aacatgccag
      901 cgccgtgaat gacgccgagg ccggcggcga cctggcgcag cgcggcgcgc aggcgttcgt
      961 ggcggacctc cggatcctcc gtcgcgtcgt cgctgtcgct gccgcgcacg tactcgacga
     1021 agccgagccc gtcgacgtac tccatcgaga agaagatctg ctcgccctcg aagaacagct
     1081 cgtgcagcgc gatcaggttc gggtgcgtga cgtcggccag caggcgaaac tcacgcttga
     1141 accgatacag cgcccccggg ctgacgttga gcagcgtctt gatcgctacc gtctcgccgc
     1201 gctcgcggtc gatcgcctcg tacaccacgc ccatgccgcc agcgccgatc cgcctgcgaa
     1261 tcgtgaaacg cttgaggctg gcgaactcga ggaactgcga gctctcgccg ggatcacgat
     1321 cggaggtttc cgcgcgtacc tcgagctcgc cgtcgttggt gtcgtcgtcg acctcgccgc
     1381 tgtcgagcgg gagatcggag cgctcgagca gcgcctccag cagcgccgac ggcttgccag
     1441 tgcccgtgcg ttgcgtgtcc tcctcgccgc tcaccctcat tgcagggtac ttcagccggc
     1501 ccgcgctggc gcgaggctcc cgccgcgggc gcccgaacgg ccgtgagacc aaccacgtcg
     1561 cttcgcgccc gcgcacgcgt cacgcacgaa acggctcaaa acccccgctg ggagtgcgct
     1621 ttcgtgagat cgcgcaagcc ccgtggccac aaggcccggg gcccggacgt ggtacccacg
     1681 gtgcgcatga cctcggatgc ccgcacgccc gtcatcgtcg ccgctgtccg tacgccgatc
     1741 ggccggtttc gcggggccct ggcgtcggtg cgcccggatg acctcgccgc cctcgtgatc
     1801 cgctcgctcg ccgagcatca cagcgcggcg atggagcagc tcgccgacgt gtacctcggc
     1861 tgcgccaacc aggccggcga ggacaaccgc aacgtcgcgc gcatggccac gctgctcgcc
     1921 ggcctgccgg tcgaggtccc cggcgtcacc gtcaatcgcc tgtgcggctc cggcatggag
     1981 gccgtgttgc aggccgcgaa gtcgatcatg gtcggcgagg gcgaggtcta catcgccggc
     2041 ggcgtcgaga gcatgacgcg cgcgcccttc gtgatgccca aggcgaccga ggcgttcgcg
     2101 cgcgaggccc agttcttcga caccgccctc ggctggcgca tggtcaaccc ggcgatggag
     2161 cgcctgttcg gcatcgagcc gctcggcatc accgccgaga acgtcgccga gcgctatcac
     2221 atcagccgcg aggaccagga cgcgtgggcc ctcggcagtc atcagcgggc cgcggccgcc
     2281 caggacgagg gctggtgcga caacgagatc atcccggtga tgatccccgg cgccaagggc
     2341 caggagccgg tccgcttcgc cgccgacgag ggcatccgcc gcgagtcctc cctcgagtcg
     2401 ctcgccaagt tgaagcccgc gttcaagccc ggtggcagcg tcaccgccgg caacagctcg
     2461 tcgctcaacg acggcgccgc cgccctgctg atcaccagcc tcgccaaggc gcacaacctc
     2521 ggcctgcgcc cgatggcccg catcgcctcg tggggccacg ccggcgtcgc tccgggcgtg
     2581 atgggcatcg gccccgtgcc ggcctcgcgc tgcgccctca agcgcgccgg cctgaccagc
     2641 gccgacatcg gcatcgccga gttcaacgag gccttcgcca gccagaccct cgcgtgcctg
     2701 cgccagctcg acctcgaccc cgccaaggtc aacccgggcg gcggcgcgat cgccctcggt
     2761 cacccgctcg gctgctcggg cgcccgcatc accgccaccc tcgcgcacag catggcccgc
     2821 acgcgcacgc gccacggcct cgccacgatg tgcatcggcg tgggccaggg catcgcgctg
     2881 gtgctagagc gcacctgagc tgagaagacg cacgcagggc cccacggccc gcgacggcgc
     2941 aaagccacgc atacgcgcct accctggtcg tatgcgcttt ttggcccgtt ctaccctggc
     3001 ttcgacgctg ctgttcgcct gcggcgacaa cggcgacatg cagatcacgc agaccaaccc
     3061 gacctcgctc gacaccggcg gatcgagcgg gacgagcggg atgagcggcg cgagcatggg
     3121 cgatacgacg acgaccggcc aaacctccac gccgccgacc accgacccga ccaccgatga
     3181 gctgacgacc gagccgctga ccaccggccc gaccaccgca ccgacgaccg acgcatcgac
     3241 ctccgaggcg accaccgagg cgaccaccga cggcagcagc ggcgacagcg gcggcgtcga
     3301 ggacctgatg aaggtgcacg gccacgcctg cggaaccgac gacgactgca aggcgctgct
     3361 cggccccgac ggcatctgtc aaaaggacat cctcggcgtc tacagcctgc cgggtgggta
     3421 ctgcaccacc tattgtcagc tcccggatca gcagaccacg tatcagctcg actcgcgttt
     3481 ttgcacgctc ggcgccgact gcgtcgggct gatgggttac ttcgaggcct gcgcgcacgt
     3541 gtgcaccgat accgcccagt gcccgcgcga gggctacgag tgccgccaga tgccgcagat
     3601 cagcaacccg gacgacccga agttctgcct gatgaccgaa gacaacatga tcccgccatg
     3661 accgtcgtcc tcctcgacgg cgccaccggc acggagatcg agc
//
LOCUS       DASWNJ010000066         2209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_212953, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000066 DASWNJ010000000
VERSION     DASWNJ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212953"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VGB85_01270"
     CDS             complement(<1..502)
                     /locus_tag="VGB85_01270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HEY0132667.1"
                     /translation="MARDQDDDDDKVSLSILELGRVRQGFDRRAALDHARDLGRHAEG
                     LGYRRIWVAEHHNMAGVTTAATSLVIQHIAAGTRTIRVGAGGIMLPNHAPLIIAEQFG
                     TLASLYPGRVDLGLGRAPGTDQWTLRALRRDPMGAESFPQDVLELQAFLAPARPNQRI
                     RAVPGMG"
     gene            complement(566..1033)
                     /locus_tag="VGB85_01275"
     CDS             complement(566..1033)
                     /locus_tag="VGB85_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132668.1"
                     /translation="MRPLRAVLALLSVCALPACGEPCANLSEALCDGGDPAYCQQVDG
                     WLASRLIDPNTKAPLEGEPRTQMCRTIHGSVDIFNAYRFKAKQKLLGEPEFTLASSTP
                     PENKADPGPKTEPKPVSVTPPTPPAVPDTPDAADDKVEDDKPGVGGGAVNMSD"
     gene            complement(1092..2021)
                     /gene="grxD"
                     /locus_tag="VGB85_01280"
     CDS             complement(1092..2021)
                     /gene="grxD"
                     /locus_tag="VGB85_01280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEY0132669.1"
                     /translation="MPISEELRSRLQAMITADEVVLFMKGNRARPQCGFSAAVVATLD
                     SLVPNYRTVDVLSDPEVREGIKELSSWPTIPQLYVRGEFVGGADIVREMQASGELEGL
                     LGADNKGPVTPPRITVSKRAAEVFREATADAGPDDAIRLTITPRFEHDLGVEPPRPGD
                     VTVEAEGIRFLLDRMSAARAEGLSFDFVEQNGQAGFKIENPNAPPSVQQMEPAELKAR
                     MAAGPVRLYDVRSPQEREIARIEGATLLDEATRDEILALERDTPLVFHCHHGGRSQQA
                     AQYFLDQGFTRVANLRGGIEAWSREVDPGVPRY"
BASE COUNT          281 a          787 c          804 g          337 t
ORIGIN      
        1 tgcccatccc cggcaccgcg cggatccgct ggttaggccg cgcgggggca aggaaggcct
       61 gcagctcgag cacgtcctgc gggaagctct cggctcccat cggatcgcgg cggagggcac
      121 ggagcgtcca ctgatcggta ccgggcgcgc ggccgaggcc aagatcgacg cggccgggat
      181 aaaggctcgc gagtgtgccg aactgctcgg cgatgatcag cggcgcatgg ttgggcagca
      241 tgatgccgcc ggcgccgacg cgtatcgtgc gggtgccggc ggcgatatgc tggatcacca
      301 gcgaggtcgc ggccgtcgtc acgccggcca tgttgtggtg ctccgcgacc catatgcgcc
      361 ggtagccgag gccctccgcg tggcggccga ggtcacgcgc gtggtcgagg gcggcccggc
      421 ggtcgaagcc ttgccgcacg cggccgagct cgaggatcga gagggacacc ttgtcgtcgt
      481 cgtcgtcttg gtcgcgcgcc atgactgctc agggtgaccc cgtctcgacg agaatcaagg
      541 ccaccccggc cgtcgcccgc cgcgactaat ccgacatgtt gaccgcgccg ccgccgacgc
      601 ccggcttgtc gtcctcgacc ttgtcgtcgg ccgcgtccgg cgtgtcgggc accgcgggag
      661 gggtcggggg cgtgacgctg accggcttgg gctcggtctt cgggcccggg tcggccttgt
      721 tctcgggcgg ggtcgacgac gcgagcgtga actcgggctc gccgagcagc ttctgtttgg
      781 ccttgaagcg gtaggcgttg aagatgtcga cgctgccgtg gatcgtccgg cacatctgcg
      841 tgcggggctc gccctcgagc ggcgccttgg tgttcgggtc gatcaggcgc gaggccagcc
      901 agccgtcgac ctgctggcag taggccgggt cgccaccgtc gcacagggcc tcgctcaggt
      961 tggcgcaggg ctccccgcag gccggcaggg cgcagaccga gagcagggcc aggacagcac
     1021 gaagggggcg catttccagg gaacgctaga ccaccgcgcc cgagcccgtc aagcgagcat
     1081 cgtgccggat ctcagtagcg cggcacgccc gggtcgacct cgcgggacca ggcctcgatg
     1141 ccgccgcgga ggttggcgac gcgcgtgaag ccctggtcga ggaagtactg ggccgcctgc
     1201 tggctgcggc cgccgtggtg gcaatggaac accagcggcg tgtcgcgctc cagcgcgagg
     1261 atctcgtcgc gcgttgcctc gtcgagcagc gtggcgcctt cgatcctggc gatctcgcgc
     1321 tcctggggcg agcgcacgtc gtacaggcgc accggcccgg ccgccatgcg cgccttgagc
     1381 tccgccggct ccatctgctg cacgctcggc ggcgcgttgg ggttctcgat cttgaagccg
     1441 gcctggccgt tttgctcgac gaagtcgaag ctcaggccct cggcccgggc cgcgctcatg
     1501 cggtcgagca ggaagcggat gccctcggcc tcgaccgtga cgtcgcccgg gcgcggcggc
     1561 tcgaccccga ggtcgtgctc gaagcgcggc gtgatcgtca ggcggatcgc atcgtcgggg
     1621 ccggcgtccg cggtcgcctc gcggaacacc tccgcggcgc gcttgctgac cgtgatccgc
     1681 ggcggcgtga ccggcccctt gttgtcggcg cccagcaggc cctcgagctc gcccgacgcc
     1741 tgcatctcgc ggacgatgtc cgccccgccg acgaactcgc cgcgcacgta cagctgcggg
     1801 atcgtcggcc acgacgacag ctccttgatc ccctcgcgca cctccgggtc ggacagcacg
     1861 tcgaccgtgc ggtagttcgg gaccaggctg tcgagcgtcg ccaccaccgc cgccgagaac
     1921 ccgcactgcg ggcgcgcgcg gttgcccttc atgaacagca ccacctcgtc ggcggtgatc
     1981 atggcttgca gacggctgcg cagttcctcg ctgatcggca tgcggccgag tgtacgcgag
     2041 cgcacgggcg acccgacagg ggcccgtgcg ccgatatgtg cgggacctcg cgcccgccgc
     2101 ggccgccgcc gctgatacgc tgcctccgtg cccgcgcctc gcctcgccct gtccgcgtgt
     2161 ctgctcctcg gcgccggcct cgcgtgcaac agcgacgacc tggacttcg
//
LOCUS       DASWNJ010000067         5027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_354503, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000067 DASWNJ010000000
VERSION     DASWNJ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354503"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..3284)
                     /locus_tag="VGB85_01285"
     CDS             complement(39..3284)
                     /locus_tag="VGB85_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0132670.1"
                     /translation="MIRVILERPEQPASLMVFAQPVVVIGRGDTDSASPVDWQLPYPD
                     VSRRHCRLSCAGGQRFVEGLSDRSPTYVNGRKVQGMTAIAAGDEIRFGYCVVRVCEDV
                     AVAPPAPAKATVAPARATAPAKATVAPAHPAPSRPAPTPTAPLADAPDEARSARFVAA
                     PAAIAEPAPAIEGDVSLLVERARHWDLRGQPTALLLTGSLLQRGRAWSRVGPELGEVG
                     GLMRLYVERSVRAQRSRLRRLGLAAGVTLTTALTGSAIAQAWLPELRIPELPSVDEAP
                     RARCREDVLARADALTSTALQQGDDAVALLLTSRALQDAAAGGCRNLASAEPTLRELL
                     AKRHSRLLDRREGPIQDAVVRRDGRYVAAADAGGHVTIWDVQGRRAAAPLDDGGVPAH
                     VLAWSRDDRWLATGTAAGEVIVWDVQEPAQAVMQPRLAGHHRPIDALAFSPEGGLLAS
                     ADADELRLWDMGGDARGDARGRYTGLAGPTSQLHFNAGASRLFGLGEDGKVRIWPIAP
                     AGPEERLGKPGTLPADGHIVAMAVNLDGTQIVTGDRSGVVLVWRLRGNTWRSSAGTTH
                     EGEIAAIHLVPGRDRFLSLAKDRTLTLVDLGAARGKQVQPPSYALGPLQNPARHLVVE
                     PSGRRALTVDDTGVAELWNLETQQKQSTRFAEQPRQVTVVTGTSAQSVVITGEVDGSL
                     RVWDLMLEGGSAGSHLLSEHRGGTPELALSHEGHTLVSTTRGKNIQVWNLDGAMVPTL
                     NDIRTASRPVEIVAVSANGRWVAGASGNFVLVWDLEKRDRNAAPANLVEHADAVMHLQ
                     FSVDGEWLVSADLRGSVRTWAINAAGPELASKHAVEHPPSVHALAVSREHVAVGTGGS
                     KDTRGEVFAWQLGESGKGNDRAVWDHAGAAHRLTFDATGSYLASGSADGGVRIGKLGD
                     DGFTVDAQYSHGQSVSALAFTALPGNGVALASGARDGEVLITTPTDPQGGSPRKLAQR
                     EGEITGLAFGVDPGLLFIGGEDGAALLQMEGTRTRTIALAGHRGAIVALHTDVVGRVV
                     VTVGDDQTLRAWPLEVSALQHIACSFVGRNLRAEELPADLRPTAEPQCPAR"
     gene            complement(3281..4327)
                     /locus_tag="VGB85_01290"
     CDS             complement(3281..4327)
                     /locus_tag="VGB85_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132671.1"
                     /translation="MISLDLPRSTRWSLLAAGLMLPLAVAMMIRLAQTPAPKHTPVRS
                     VPAFPALCDATRLDGRCADDQHCVADICHPLRRGARGTEGTLCRGDLCDAGLECFHGR
                     CVSWDRLPRAPEVCRAGPTRASLEYLRSRCAADLGQVDAPLTACTAKTWEPLSRRDPT
                     FAGHIEALTHVFTVHFPQGEPGPRGTWATPEIRATYLRQLAPHHAALRDAKALLVIGR
                     ASVEGSEELNRALSERRTALVVGLLRSALGDAAPPIHAWALADQDALPPEQFMRVMKT
                     AVLAWDEAITRRLLTQTDLAERSGPDWQWFLEVVNRAVLIVPLYCDGREYYPQPSFQG
                     AADPHRSAQPRELP"
     gene            complement(4324..4680)
                     /locus_tag="VGB85_01295"
     CDS             complement(4324..4680)
                     /locus_tag="VGB85_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132672.1"
                     /translation="MRAVECVERLAPGAGIRAVGLAFDAQWQPERDLLADMTSLGVDG
                     DTLRRCVAADREVDARLAAHAEAARRHGLVEAPAGFVIVPGASPRVSPFGAWLTETSL
                     RTVMQCLVDARCQEGP"
BASE COUNT          697 a         1877 c         1752 g          701 t
ORIGIN      
        1 gcggtccgcg tgagtctggc tgtcgcggct gacccgggtc accgcgccgg gcactgaggt
       61 tcggcggtcg gacgcaggtc cgcgggcagt tcctcggcac gcaggttgcg tccgacgaac
      121 gagcacgcga tgtgttgcag cgcggagacc tcgagcggcc acgcgcgcag ggtctggtcg
      181 tcgccgacgg tgacgaccac gcgtccgacc acatccgtgt gcagcgcgac gatcgccccg
      241 cggtgaccgg ccagggctat cgttcgggtc ctcgtaccct ccatctgcaa gagcgccgcg
      301 ccgtcctcgc cgccgatgaa cagcagcccc gggtccacgc cgaacgcgag accggtgatc
      361 tccccctcgc gctgcgccag cttgcgcggc gacccgcctt gcgggtccgt cggtgtggtg
      421 atcaagacct caccgtcgcg ggcccccgag gccagcgcga cgccgttgcc cggcaacgcc
      481 gtgaacgcca gcgcgctcac cgattgaccg tggctgtact gcgcgtcgac ggtgaagcca
      541 tcgtcgccga gcttcccgat cctgacaccc ccatcggcgc tgcccgaggc gagataactc
      601 ccggtggcat cgaaggtcag gcgatgcgcc gcgccggcgt ggtcccacac cgccctgtcg
      661 ttgcccttcc ctgactcgcc gagttgccac gcgaacacct ctccgcgcgt atccttgctg
      721 ccgccagtgc cgaccgcgac atgttcgcgc gagactgcca gcgcgtgcac gctcggcggg
      781 tgctcgaccg cgtgcttcga cgccagctcc ggacccgccg cgttgatcgc ccacgtacgc
      841 accgagccgc gcaggtcggc cgagaccagc cactctccgt cgacgctaaa ttgcaggtgc
      901 atcaccgcgt cggcgtgctc gaccaggttc gccggcgcgg cgttcctgtc gcgcttctcg
      961 aggtcccata cgagtacgaa attcccggac gcgccggcga cccagcggcc gttcgcgctg
     1021 accgcgacga tctcgaccgg ccgcgatgcg gttcgaatgt cattgagcgt cggcaccatc
     1081 gcgccgtcga ggttccacac ctgaatgttc ttgccgcggg tcgtgctgac cagcgtgtgg
     1141 ccctcgtggc tcagcgcgag ctccggcgtg cccccgcggt gttcgctgag cagatgagac
     1201 cccgcgctgc cgccctcgag catcaggtcc caaactcgca agctcccgtc gacctcgccg
     1261 gtgatgacga cggactgcgc cgaggtcccg gtcaccaccg tcacctggcg cggctgctcg
     1321 gcgaagcgcg tcgactgctt ttgttgggtc tcgaggttcc acagctcggc gacgccggtg
     1381 tcgtcgaccg tcagcgcgcg gcgccccgag ggctcgacca ccaggtgccg cgccgggttc
     1441 tgcagcggcc ccagcgcgta ggaaggaggc tggacctgct tgcctcgcgc cgcgccgagg
     1501 tcgacgagcg tcagggtgcg atctttggcg agcgacagga accggtcgcg tcccggtacg
     1561 aggtggatcg ccgcgatctc gccctcgtgg gtcgtcccgg cgctgctgcg ccaggtgttg
     1621 ccgcgcagtc gccacacgag caccacgccc gagcgatcgc cggtcacgat ctgcgtcccg
     1681 tcgaggttga cagccatggc gacgatgtga ccgtcggccg gcaacgtgcc cggcttaccg
     1741 agccgctcct ccggtcctgc cggcgcgatc ggccagatcc tcaccttgcc gtcctcgccg
     1801 agcccgaaga gtcgtgaggc gccggcgttg aagtggagct gagaggtcgg tcccgccagc
     1861 ccggtgtacc tgccgcgcgc gtcgccgcgc gcgtcgccgc ccatgtccca cagccgcagc
     1921 tcgtccgcgt ccgcgctggc cagcagaccg ccctcggggc taaacgcgag cgcgtcgatc
     1981 ggccggtggt ggcccgcgag gcgcggctgc atcaccgcct gcgccggttc ctggacgtcc
     2041 cacacgatga cctcgcccgc cgccgtgccc gtcgccagcc agcggtcgtc acggctccac
     2101 gcgagcacgt gcgccggtac cccgccgtcg tcgagcgggg ccgcggcccg ccgcccctgg
     2161 acgtcccaga tcgtgacgtg cccaccggcg tccgcggcgg cgacgtagcg accatcgcgg
     2221 cgcaccaccg cgtcctggat cgggccctca cggcggtcga gcaggcggct gtggcgcttg
     2281 gcgagcagct cgcgcagggt cggctcggcc gacgcgaggt tgcgacatcc gccggccgcc
     2341 gcgtcttgca gcgcccggct cgtcaacagc agcgcgaccg catcgtcacc ctgttgcagc
     2401 gcggtggacg tcaacgcgtc cgcgcgcgcc agcacgtctt cccggcatcg cgcgcgcggg
     2461 gcctcgtcga ccgacggcag ctccgggatg cgcagctccg ggagccacgc ctgggcgatc
     2521 gccgagccgg tgagcgccgt cgtcagcgtc acgccggcgg ccaggccgag acgtcgcagc
     2581 cgggaccgct gcgcgcgtac gctgcgctcg acgtagagac gcatcaggcc gccgacctcc
     2641 ccgagctccg gcccgacccg cgaccacgcg cgcccgcgct gcaacagcga gccggtcagc
     2701 agcagcgccg tcggttgccc gcgcaggtcc cagtgccgcg ctcgctcgac gaggagcgag
     2761 acgtccccct cgatcgcggg cgcgggctcg gcgatcgcgg ccggtgccgc gacgaaccgg
     2821 gccgaacgcg cctcgtccgg cgcgtccgcc agcggcgcgg tcggagtcgg ggccggcctc
     2881 gacggggcag ggtgcgcagg agcgacggtg gcttttgcgg gggcggtggc tctcgcggga
     2941 gcgacggtcg ctttcgcggg agccggcgga gcgaccgcga cgtcctcgca tacccgcacg
     3001 acgcagtagc cgaagcggat ctcgtcgccc gccgcgatcg cagtcattcc ctggaccttg
     3061 cggccgttga cgtaggtcgg gctgcgatcg ctcagaccct cgacgaagcg ttgaccgcct
     3121 gcgcacgaga gccggcaatg tcgacgtgag acatcggggt agggaagttg ccagtcgacc
     3181 gggctggcgc tgtcggtatc tccgcggccg atcaccacca ccggctgcgc gaagaccatg
     3241 agcgaggcgg gctgttctgg gcgttcgagg atgacgcgga tcatgggagc tccctgggtt
     3301 gggcggaccg gtgcggatcc gcagcgccct gaaacgaggg ctgcgggtaa tactcgcgcc
     3361 cgtcgcagta cagcggcacg atcaggacgg cgcggttgac cacctcgagg aaccattgcc
     3421 agtccggccc cgagcgctcg gcgaggtcgg tttgcgtcag gagccgacgg gtgatcgcct
     3481 catcccacgc cagtaccgcc gtcttcatga cacgcatgaa ttgctccggg ggcagcgcgt
     3541 cctggtcggc gagcgcccac gcgtggatgg gcggcgccgc gtcgcccagc gcgctgcgca
     3601 gcagccccac gaccagcgcg gtgcggcgct cggacagcgc ccggttgagc tcctcggacc
     3661 cctcgacgct cgcacgcccg atcaccagca aggccttcgc gtcgcgcagc gccgcgtgat
     3721 gcggcgccag ctgccgtaga taggtggcgc ggatctccgg cgtcgcccag gtcccccgcg
     3781 ggcccggctc gccctgggga aagtggacgg tgaatacgtg cgtcagcgcc tcgatgtggc
     3841 cggcaaacgt cgggtcgcgt cgactcagcg gctcccaggt cttcgccgtg cacgcggtca
     3901 gcggcgcgtc gacctgcccg aggtcggccg cgcagcgcga gcgcaggtac tcaagggaag
     3961 cgcgcgtcgg ccccgcgcga cacacctccg gcgctcgggg caggcggtcc cacgacacgc
     4021 agcggccatg aaagcactcg agccctgcgt cgcacaggtc tcctctacat agagtcccct
     4081 cggtgcctcg cgcgccccgg cgcagcggat ggcagatgtc ggcgacgcag tgctggtcgt
     4141 ctgcacagcg gccatcgagg cgggtggcgt cgcacagggc cgggaaggca ggaacggagc
     4201 gcaccggcgt gtgcttcggc gccggcgtct gcgcgagccg gatcatcatc gccacggcga
     4261 gcgggagcat cagccccgcc gccagcagcg accaccgcgt cgagcgcggc aggtcgagcg
     4321 agatcacgga ccctcctggc agcgcgcgtc gacgaggcac tgcatcacgg tccgcagcga
     4381 cgtctcggtc agccacgccc cgaacggcga gacccgcggc gatgctcccg ggacgatcac
     4441 gaagccggcc ggcgcctcga cgaggccgtg acggcgcgcc gcctcggcgt gggccgccag
     4501 ccgggcgtcg acctcgcggt cggcggcgac gcagcgccgc aacgtatcgc cgtcgacgcc
     4561 gaggctcgtc atgtcggcca gcagatctcg ctccggttgc cactgcgcgt cgaaggcgag
     4621 gccgaccgcc cggatcccgg cgcccggcgc caggcgctcg acgcactcga ccgcacgcac
     4681 cgcctcgcaa ccggccgaac gccgggccgg tccctcgcaa gcgcctggcg catgcaacac
     4741 acgcagctcg gtcgcgtgcc cggtcgtgcc ggccgccagc gcccgggtga gtcgctgaaa
     4801 aatttgccgc gaccgcaccg aattctgatc gatgaatagc gcgatcgaca ctgtaggagc
     4861 gggcgcgccg taacggatcg catctggcag cccgaatccc tcgcgtcgcc ccgcgcagtc
     4921 cccagcttca tgagaacagc tcggatgggg ctgtgtacgc tgttcctccc ggtctcgtgt
     4981 ccacacgtgg gcgagcatcc atactgccag cgctgtcagc gccacat
//
LOCUS       DASWNJ010000068         3813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_107340, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000068 DASWNJ010000000
VERSION     DASWNJ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107340"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VGB85_01300"
     CDS             <1..451
                     /locus_tag="VGB85_01300"
                     /inference="COORDINATES: protein motif:HMM:NF015657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132673.1"
                     /translation="WVLGTEKMHIPAGEPDAVQSFELDMTGVVGFIFPNGPLTTDAPF
                     VVHSAGLHQHTLGTRSTLEVIRGGGAGDECLLDIPRWDFDWQGTYELAEPVTVNPGDR
                     IRVECHWNNSAANQPIVDGVQQPPKDVGWGEGTGDEMCLGLMYVTAP"
     gene            complement(530..1870)
                     /locus_tag="VGB85_01305"
     CDS             complement(530..1870)
                     /locus_tag="VGB85_01305"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0132674.1"
                     /translation="MRVAAKYVPLALALAAACERDDLAPIVPGPPDLPSPPEPLAPRC
                     GDGLANDVEVCLRSDAVLLPYAHRFIRYADFDGNGQRDYFAINTHFEVFWPLFFTGGF
                     DNGGPKPVTYAAFPNHEIFRAEVLDFNGDGRSDLVGATQFYYPSGDIVTTETRLLVWR
                     NVGNYEFEEAQNTPVELASFAAGDIDGDGLTELLAVVAGTGGYVWAYVPDTDTIEYVW
                     DLDLKPLQVVGSPVVVAADYNGDIHADFVLMDGSGRAWRIVGGPDHQLTILDPQAPVV
                     LTPESETLYAPDLDGDGITDLAAVRRISNSRDHTISLALGQPGGGFTALASFDTGHDL
                     TYFGGGFFGGLPEHKHLGFYDLDGSGELALVYAPYRQPALIIHPRIAETLGATPTIIP
                     LDFPANSVFVDDFEGDGEDAIYVTMSDNVKVKPSKEVPGGVISTHYLVRFTPDP"
     gene            complement(1876..>3813)
                     /locus_tag="VGB85_01310"
     CDS             complement(1876..>3813)
                     /locus_tag="VGB85_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132675.1"
                     /translation="AVAEVRALAWELAVAQVGHSRGTLLLRADSGAMLAFSPDAHYSP
                     DDEVAVKLRARTFASDILAYNDPDQQFVADIDAAPVTGGPLLGSTYTPAACEEDPNAL
                     PACGQTRLGNREIVVLDADHQNFYGDKTSHPRIGASPTGQFLATPPATGDDDSPMRDD
                     AALQDFFYRLQATYSLIDGFHAGHWDHRDDKMSSFPAQKYKPRVVLAHNTDVLLTCGI
                     SQPGCTKMYFPNIVDPMMLVPIYDEHPEADLPPHVWAESDIHAEPMGFIGTAVDGFTS
                     PDLVFHEFGHVVDLFTMPGFIRSGIGSGCTKEMQGGGKCVASCVLDSTDEAEALAETV
                     ADMIDLFSVGQLYTTVEYGRCEAISAMTGVFGPVHDPACVKGPEDIKSFLDQRPDEPG
                     MVEVDGVWLPTGKCVKSPGYRQSGILQAWWEWTHARDCLPGEPFACEEFKDESEGARS
                     GIEALLFAMSQTNATYYRKLFTDMEVYLACTEGPAEASRFRGVFCHHGALDCGGLPPI
                     CPATCGDGKAELTEACDGADLRSQSCFDHGFVGGALGCHDDCTFDLSLCTDDVSTTVE
                     PWPNPTTPTTGADVPTTGANPPATSASEATSGEDSVSGDHAEDGCGCHHGSNSLSSLL
                     SLALLALRRRRVDPIASAGVR"
BASE COUNT          635 a         1218 c         1338 g          622 t
ORIGIN      
        1 ctgggtcctc ggcaccgaga agatgcacat ccccgccggc gagcccgacg cggtccagag
       61 cttcgagctc gacatgaccg gcgtcgtcgg cttcatcttc cccaacggcc ccctgacgac
      121 cgacgccccc ttcgtcgtcc actccgccgg tctgcatcag cacaccctcg gcaccaggag
      181 cacgctcgag gtcatccgcg gcggcggtgc cggcgacgag tgcctgctcg acattccccg
      241 ctgggacttc gactggcagg gcacctacga gctcgccgag ccggtgaccg tcaaccccgg
      301 cgaccgcatc cgcgtcgagt gccactggaa caactccgcc gccaaccagc cgatcgtcga
      361 cggcgtgcag cagccgccca aggacgtcgg ctggggcgag ggcaccggcg acgagatgtg
      421 cctcggcctg atgtacgtca cggccccgtg agcccgtcga gttgtgcctg atgccccgcc
      481 ggaggggctg tgcaggacgt tgccttcgcc ctgatcgatc gctcgcgggt cacgggtccg
      541 gggtgaagcg gacgaggtaa tgcgtgctga tcacgccgcc agggacctcc ttggacggct
      601 tgaccttgac gttgtcgctc atcgtgacat agatcgcgtc ctcgccgtcg ccctcgaagt
      661 cgtcgacaaa cacgctattg gcagggaagt cgagcgggat gatggtcggc gtggcgccga
      721 gagtctcggc gatgcgcgga tgaatgatga gcgcgggttg ccggtagggg gcatagacga
      781 gcgcgagctc gccgctaccg tcgaggtcgt aaaagccgag gtgcttgtgc tccgggaggc
      841 caccaaaaaa gccgcctcca aaatacgtga ggtcgtgccc ggtatcgaag ctggcgagcg
      901 ccgtgaaccc cccgcccggc tgcccgagtg cgaggctgat ggtgtggtcc cgactgttcg
      961 agatacggcg cacggctgcg aggtccgtga tgccgtcgcc gtcgaggtcg ggtgcgtaaa
     1021 gggtctccga ttcgggcgtc aggaccaccg gcgcctgtgg gtccaatatc gtgagctgat
     1081 gatcgggacc gccgacgatc cgccacgccc ggccggagcc gtccatcaag acaaagtcgg
     1141 cgtggatgtc gccattataa tcggcggcca ccaccaccgg cgaaccgacg acctgcaagg
     1201 gcttgaggtc gagatcccag acgtattcga tcgtatcggt gtcggggaca taggcccaga
     1261 cgtagccccc ggtcccagca acgacagcaa ggagctccgt gaggccgtca ccgtcgatat
     1321 cgccggccgc gaaactggcg agctcgaccg gcgtattttg ggcctcttca aattcatagt
     1381 tgccaacatt gcgccacacg aggaggcggg tctcagtggt cacgatgtcc ccggaagggt
     1441 agtagaactg cgtggcgccg acgaggtcgc tgcgcccatc accgttgaag tcgagcacct
     1501 cggcgcggaa gatctcgtgg ttggggaacg ccgcgtaggt tacaggcttt ggtccgccgt
     1561 tgtcgaagcc gccggtaaag aagagcggcc agaacacttc gaagtgggtg ttgatcgcga
     1621 aataatcgcg ctgtccgttg ccatcgaaat cggcataccg aatgaacctg tgggcatacg
     1681 gcagcaagac cgcgtcgctg cgcaggcaca cctccacgtc gtttgcgagg ccgtctccac
     1741 accgtggcgc gagcggttca ggtgggctgg gcagatccgg cggtccgggg acaatcgggg
     1801 cgaggtcgtc gcgctcacac gccgcggcga gcgcgagcgc cagcgggacg tatttcgcgg
     1861 ccacgcgcat cggcctcatc gtacacctgc gctggcgatg gggtccacgc gccgacgacg
     1921 aagggccagc agagccagcg agagcaggct gctcagggag ttggagccat ggtggcagcc
     1981 gcaaccgtcc tcggcgtgat cgccgctaac cgaatcctcg ccggacgttg cttcgctggc
     2041 cgatgtggcc ggcgggttcg cgccggtggt cggcacatcg gcgccggtgg tcggcgtggt
     2101 cgggttcggc cagggttcga cggtggtgct cacatcatcc gtacacagcg agagatcgaa
     2161 ggtgcaatcg tcgtggcagc ccagggcgcc gccgacaaac ccgtgatcga agcagctttg
     2221 gctgcgcagg tcggcgccgt cgcaggcctc ggtgagctcg gctttgccat ccccgcaggt
     2281 cgccggacat atcggcggca ggccgccgca gtccagggcg ccatgatgac aaaacacgcc
     2341 gcgaaaccgc gaggcctcgg ccgggccctc ggtgcaggcg aggtagacct ccatgtccgt
     2401 gaacagcttg cgatagtagg tcgcgttggt ctggctcatg gcgaacagca gcgcctcgat
     2461 gccggagcgg gcgccctccg actcgtcctt gaattcctcg caggcgaatg gttcgccggg
     2521 caaacagtcg cgggcgtggg tccactccca ccaggcctgg aggataccgc tctggcgata
     2581 gccgggcgac ttgacgcact tgccggtcgg cagccacacg ccatcgacct cgaccatgcc
     2641 aggttcgtcg ggccgctggt cgaggaagga cttgatgtcc tccggaccct tcacgcaggc
     2701 ggggtcatgg acgggaccga acaccccggt catggcgctg atggcctcgc agcggccata
     2761 ctcgacggtg gtgtacagct ggccgacgct aaacaggtcg atcatgtcgg cgaccgtctc
     2821 cgcgagggcc tcggcctcgt ccgtgctgtc gagcacgcac gacgcgacgc acttacctcc
     2881 gccctgcatt tctttggtgc agccgctccc gatcccggag cggatgaatc ccggcatggt
     2941 gaacaggtcg acgacgtgac cgaactcgtg aaacacgagg tccgggctcg tgaacccgtc
     3001 gaccgcggtg ccgatgaagc ccatgggctc ggcgtggata tcgctctccg cccacacgtg
     3061 aggcggcaag tcggcctcgg ggtgctcgtc gtagatcggg accagcatca tggggtcgac
     3121 gatgttcggg aagtacatct tggtgcaccc cggctgggag atgccgcagg tgagcagcac
     3181 atcggtgttg tgggcgagga cgacgcgcgg cttgtacttc tgcgcgggga agctcgacat
     3241 cttgtcgtcg cggtggtccc agtggccggc gtggaagccg tcgatcagcg agtaggtcgc
     3301 ctgcagccgg tagaagaagt cttgcagggc ggcgtcgtcg cgcatcggcg agtcgtcgtc
     3361 gccggtcgcg ggcggcgtgg cgaggaactg gcccgtcggg ctcgcgccga tgcgcgggtg
     3421 ggaggtcttg tcgccgtaga agttctggtg gtcggcgtcg aggacgacga tctcgcggtt
     3481 gccgaggcgg gtctgaccgc aggcaggcag cgcgttcggg tcctcctcac aggcggccgg
     3541 ggtgtaggtc gacccgagca gcggaccgcc cgtgaccggc gccgcgtcga tgtcggcgac
     3601 gaactgctgg tccgggtcgt tgtacgcgag gatgtcgctg gcgaaggtcc gggcccgaag
     3661 cttcaccgcg acctcgtcgt ccgggctgta gtgggcgtcg ggcgagaagg ccagcatcgc
     3721 gccgctgtcg gcgcgcagca ggagcgtgcc gcggctgtga ccgacctgag cgaccgcgag
     3781 ctcccaggcg agcgcacgga cctcggcgac agc
//
LOCUS       DASWNJ010000069         1873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_72021, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000069 DASWNJ010000000
VERSION     DASWNJ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72021"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VGB85_01315"
     CDS             <1..555
                     /locus_tag="VGB85_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEY0132676.1"
                     /translation="TDTAEDVFLLTLNEVIAAADAHARGESLVGLGELIAQRRITQAR
                     DAGRTPPSIMTDEGETPPLPAPAAAPPGTLAGVGVSSGVIEGRARVIRDPGSETLAAG
                     EILVAPFTDPGWTPLFMHASGLVMEVGGLMTHGSVVAREMGIPAVVAIEGATRTIVSG
                     QRIRVDGDLGWVTILDNDDDGGAP"
     gene            552..1031
                     /locus_tag="VGB85_01320"
     CDS             552..1031
                     /locus_tag="VGB85_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132677.1"
                     /translation="MRGALLFMLVPLAAMLVMVAYTRRFGELWTASLAQLGGLAPLLV
                     IAVIFAAGVEVLVPAQKIGRWLGEESGARGIGIAWAAGILTPGGGPIGLTLVAGVAKQ
                     GASTPVLLTYLVSLSTLSLIRVPLEAAIIGGRLTTLRVVACLLLPPVVGVGARLLRL"
     gene            1412..>1873
                     /locus_tag="VGB85_01325"
     CDS             1412..>1873
                     /locus_tag="VGB85_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132678.1"
                     /translation="MIEAGAYAEGVVELERAYALLPDPLVHRAGRSKVLGSIRSVLVR
                     LHGATGDPAHLERLRDHLLLYLEGLLLALGEQATADDAVGALTVLREVSGKLAKQRPS
                     PPPEPTVIAVRPVRVRPRVAMAPQKVEGPRLASGPPASTSGRPLRFAGAVLI"
BASE COUNT          236 a          568 c          758 g          311 t
ORIGIN      
        1 acggacacgg ccgaggatgt gtttctgctg acattgaacg aggtgatcgc ggccgccgac
       61 gcgcacgcgc gcggggagtc gctcgtgggc cttggagaat tgatcgcgca acgaaggatc
      121 acgcaggcgc gggacgccgg gcgcacgccg ccgtcgatca tgactgacga gggcgagaca
      181 ccgccactgc cggccccggc cgcggcgccg ccggggacgc tggcgggggt cggggtgtcg
      241 tcgggggtga tcgagggccg ggcccgggtg atccgcgatc cgggcagcga gacgctggcg
      301 gcgggcgaga tcctggtcgc cccgttcacc gatccggggt ggacgccgct gttcatgcac
      361 gcatcggggc tggtgatgga ggtcggcggg ctgatgacgc acggcagcgt ggtcgcgcgt
      421 gagatgggga tccccgcggt ggtggcgatc gagggcgcga cgcggacgat cgtcagcggg
      481 cagcggatcc gcgtcgacgg tgacctcggg tgggtgacga tcctcgataa tgacgacgat
      541 ggcggggcgc cgtgaggggc gcgctgctgt tcatgctcgt gccgctggcg gcgatgctgg
      601 tgatggtcgc gtacacgcgg cgcttcggcg agctgtggac cgccagcctc gcgcagctcg
      661 gagggctcgc gccgttgctg gtgatcgcgg tgatcttcgc ggccggggtc gaggtgctgg
      721 tgccggcgca gaagatcggg cgctggctcg gcgaggagtc gggcgcgcgt gggatcggga
      781 tcgcgtgggc ggcggggatc ctgacgcccg gcggcggacc gatcgggctg acgctggtcg
      841 ccggtgtggc aaagcagggg gcgtcgacgc cggtgctgct gacgtacctg gtgtcgctgt
      901 cgacgctgtc gctgatccgc gtgccgctcg aggcggcgat catcggcgga cgactgacga
      961 cgctgcgcgt ggtcgcgtgc ctgctgctgc cgccggtcgt cggcgtcggg gcgcggctgc
     1021 tgcggctgtg atccgcgagc gccaggcagc acgtggaagc tgccgattct tctcctgaaa
     1081 cagatcgtgt aatttgccat acatataatg cagtcattga tgttcgtttg ggagtgacgc
     1141 gcgcggatcg cttgacacgg cgaggttttg cttcgacgtt gtgcgcgtga ggcatgggag
     1201 ctacttcata tcgagtcggt cgcgcacctc gtcgaggctg cggggagccg gggatggaga
     1261 ccggcgcgct cgcggggaag gatcgaggat cgccgcgctc gtgatcgtcg ctgtggcgat
     1321 ggtttgtgcc ggggcggctt cggccgccac gcgcgaaccg aggtcggatc tcacccccga
     1381 ggcccggccg ttcaacagtc gcggcatcgc cttgatcgag gccggtgcgt atgccgaggg
     1441 ggtggtcgag ctcgagcgag cctacgcgct gttgcccgat ccgctggttc atcgcgcggg
     1501 tcgcagcaag gtgctcgggt caatccgcag cgtgctcgtc aggctccacg gggcgaccgg
     1561 agatccggcg cacctcgagc ggctgcggga ccatctcctt ctgtacctcg agggtctgct
     1621 gctcgcgctg ggtgagcagg cgaccgcgga cgacgcggtt ggcgcgctga cggtgctgcg
     1681 cgaggtgtcc gggaagctgg cgaagcagcg gccgtcgccg cctcccgagc cgaccgtgat
     1741 cgcggtccgt ccggtgcgag tgaggccgcg ggttgcgatg gcgccgcaga aggtcgaggg
     1801 accgcggctc gcctccggcc cgcccgcgtc gacgtcgggg cggcctctcc gtttcgcggg
     1861 cgccgtcctg atc
//
LOCUS       DASWNJ010000070         2112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_142769, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000070 DASWNJ010000000
VERSION     DASWNJ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142769"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="VGB85_01330"
     CDS             complement(<1..1259)
                     /locus_tag="VGB85_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEY0132679.1"
                     /translation="MPAPSTDPLLDHAWQRDPGAVPLAERMRPRNLGEFAGQAHLVGP
                     GRMLARVAETGRVPSLIFWGPPGVGKTTLARLLAEHTRSHFVVLSATAAGVKELREVV
                     EQARRRRAHEGRGTLLFVDEIHRFNKGQQDVLLPHLEAGACALVGATTENPSFEVNAA
                     LLSRVRVLQLRPLAIADIVGLMRRALADRERGLGAREITVDEGLLAAIAHTAQGDARR
                     ALNSLELAVDLLPDDEHVLGKEVAAQAVGARSLRYDRAGEEHYNVVSAFIKSMRASDA
                     DAAVYWLARMLEAGEDVDFVARRLVIFASEDVGNADPRAISVASACYDIVERIGMPEA
                     KITLAQAVVYMATAPKSNRSYAAINAAIDDVKHGRTIPVPKHLRDGHYAGSARLGHGK
                     GYKYAHDYAGGYVAQDYLGVDKTYYEP"
     gene            1281..1853
                     /locus_tag="VGB85_01335"
     CDS             1281..1853
                     /locus_tag="VGB85_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132680.1"
                     /translation="MFAPLAPRRLRSGPSRLAAARTGVLLAVLGLGATAGCDEKLQAY
                     LKNASERQSARDAKKAEDAALEARGMGPEDPISPEIARAIEVVRTSDYDFIVRKGKAP
                     RKKKKPEDDTAKRFNGFDFAATLESKTRWLGRGVTDLPTWLDEIGSGTFFSGDAYLVR
                     LPDGRELTFREWLLEELAALPPDVLETPKP"
     gene            1850..>2112
                     /locus_tag="VGB85_01340"
     CDS             1850..>2112
                     /locus_tag="VGB85_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEY0132681.1"
                     /translation="MSAKIGIILAGLFGFIGVAAGAFGAHGLRDKVSPRDLEIWHTGA
                     HYQQLHAVVLLVVALWAMQARTGGATGLHGVAMTLFTAGIVIFS"
BASE COUNT          291 a          763 c          741 g          317 t
ORIGIN      
        1 ctcggctcgt aatatgtctt atcaaccccc aggtaatcct gcgcgacata ccctccggca
       61 tagtcatggg cgtacttgta ccccttgccg tgccctagcc gggcagaccc ggcgtagtgc
      121 ccgtcgcgga ggtgcttggg gacggggatc gtccgcccgt gcttcacgtc gtcgatcgcc
      181 gcgttaatcg ccgcgtacga ccggttgctc ttgggcgcgg tggccatgta gacgacggct
      241 tgggcgagcg tgatcttcgc ctcgggcatg ccgatccgtt cgacgatgtc gtagcaggcg
      301 gaggcgaccg agatcgcgcg ggggtcggcg ttcccgacgt cctcgctggc gaagatgacg
      361 agccggcgcg cgacgaagtc gacgtcctcg ccggcctcga gcatgcgcgc gagccagtac
      421 accgccgcgt cggcgtcgct ggcccgcatg ctcttgatga acgcggagac gacgttgtag
      481 tgctcctcgc cggcccggtc gtagcgcagg ctgcgggcgc cgaccgcctg cgcggcgacc
      541 tccttgccga gcacgtgctc gtcgtcgggc aggaggtcga cggccagctc gaggctgttg
      601 agggcccgtc gcgcgtcgcc ctgcgcggtg tgggcgatcg cggccaggag accctcgtcg
      661 acggtgatct ctcgggcgcc gaggccccgc tcgcggtccg cgagcgcgcg gcgcatcagg
      721 ccgacgatgt cggcgatcgc cagcggccgc agttgcagca cgcgcacccg gctcagcagc
      781 gccgcgttga cctcgaagct cgggttctcg gtcgtcgcgc cgaccaatgc gcaggcgccg
      841 gcctcgaggt gcggcaacag gacgtcctgc tggcccttat taaaacggtg gatctcgtcg
      901 acgaacagca gcgtgccacg accctcgtgg gcgcggcggc gccgggcctg ctcgacgacc
      961 tcgcgcagct ccttgacccc cgcggcggtc gcggacagca cgacgaagtg gctgcgcgtg
     1021 tgctccgcca gcaggcgcgc gagggtcgtc ttgccgaccc ccggcggccc ccagaagatc
     1081 aggctcggca ccctcccggt ctccgcgacc cgcgcgagca tccgcccggg accgacgagg
     1141 tgggcctggc cggcgaactc gccgaggttg cgcggccgca tccgctccgc caggggcaca
     1201 gcgcccgggt cgcgctgcca ggcgtgatcg aggagcgggt cggtcgaggg agcgggcacg
     1261 gccgtgctag cctagcaggc gtgttcgccc ccctggcccc gcgccgcctc cgatctggac
     1321 catctcgcct cgccgcggcg cggaccggcg tgctgctggc cgttctcggg ctcggggcga
     1381 ccgccggctg cgacgagaag cttcaggcct acctgaagaa cgcctcggag cggcagtccg
     1441 cgcgggacgc gaagaaggcc gaggacgccg cgctggaggc ccgcggcatg ggccccgagg
     1501 acccgatctc gccggagatc gcccgcgcga tcgaggtcgt gcgcaccagc gactacgact
     1561 tcatcgtccg caagggcaag gcgccgcgca agaagaagaa gcccgaggac gacaccgcca
     1621 agcgcttcaa cggcttcgac ttcgcggcca ctttggagag caagacccgg tggctcggcc
     1681 gcggcgtcac cgacctcccg acctggctcg acgagatcgg ctcgggcacc ttcttctcgg
     1741 gcgacgccta cctcgtgcgc ctcccggacg gccgcgagct gacctttcgc gagtggctgc
     1801 tcgaggagct cgccgccctg ccccccgatg tcctggagac ccccaagcca tgagcgcaaa
     1861 gatcggcatc atcctcgcgg gcctgttcgg cttcatcggc gtcgccgccg gggcctttgg
     1921 cgcccatggt ctgcgcgaca aggtcagccc gcgtgacctc gagatctggc acaccggcgc
     1981 ccactatcaa cagctgcacg cggtcgtcct gctggtcgtc gcgctgtggg cgatgcaggc
     2041 ccgcaccggc ggcgcgaccg gtctgcacgg cgtggcgatg acgctgttca ccgccgggat
     2101 cgtgatcttc tc
//
LOCUS       DASWNJ010000071         2152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_566524, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000071 DASWNJ010000000
VERSION     DASWNJ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566524"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VGB85_01345"
     CDS             complement(<1..211)
                     /locus_tag="VGB85_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132682.1"
                     /translation="MTLPPGPGPSHASSSLEPSPRPRRGAWLAALAGLALPVLPTALL
                     LSGLACDGDAAMARAVDEPAAVATAA"
     gene            272..1120
                     /locus_tag="VGB85_01350"
     CDS             272..1120
                     /locus_tag="VGB85_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY0132683.1"
                     /translation="MQSFTGTSEYIVSPELARAVDVAVVLGRPLLVKGEPGTGKTLLA
                     RDIARFFNAPLERWHVKSTTKAVDGLYQYDAVRRLHDSRFGDGEVRDIARYITLGPLG
                     RAFRRDERVVVLIDEIDKADLEFPNDLLHELDTMSFTISETGETVEALHRPIVIITSN
                     NEKELPDAFLRRCVFHWIEFPDRALMQRIVRVHHADAEEQLLASALEAFYTLRAVDGL
                     RKPPSTSELIDWVSALRHSGMDPAKLLGAGLPFIGTLLKKEQDIDTAQRRFNAPQGTR
                     GGRTGH"
     gene            1120..>2152
                     /locus_tag="VGB85_01355"
     CDS             1120..>2152
                     /locus_tag="VGB85_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0132684.1"
                     /translation="MFLDFFYKLRERGVPVTTHTWLALIEALAKGLHQDSLDGFYQVA
                     RCVLIQNEAHYDDFDRVFAESFRGVAADARDLLSEIEKWLADPKLLEHLDPELRAQLK
                     GMDLEELRKQLLERLEKQKERHDGGSHWVGTGGTSPFGTGGAHPTGIRVGGGGGRSAL
                     AVADARRFREFRRDLVLDTRQIAAALRRLRRLTRDDGKLVLDLDETIDATARNCGDIE
                     VVLNPERHADVRLLLLLDVGGSMDPHAQLVSRLFSAAHQGGGFKELRSYYFHNCVYQR
                     VFEDAAFTRPVQTAELIKTLDTKWTVVIVGDAYMHPGELSMTSGDWWESTRGPSGLSW
                     LARLADRFPR"
BASE COUNT          342 a          737 c          766 g          307 t
ORIGIN      
        1 atgcggcggt ggcgaccgcg gccggctcgt cgaccgcgcg cgccatcgcg gcgtcgccgt
       61 cgcaggcgag cccggacagc aacagcgcgg tcggcagcac cggcagggcg aggccggcga
      121 gcgccgccag ccaggcgccg cggcgcggac gcggggacgg ctccagggag ctggaggcgt
      181 gggacggacc ggggccggga ggaagggtca cggggcgggg catggggctg gctttgataa
      241 cgtccgggct ttcgtgccat catcccgggc ggtgcagagc ttcacaggga cctccgaata
      301 catcgtttct ccggagctgg cgcgggccgt ggacgtcgcg gtggtgctgg ggcggccgtt
      361 gctggtgaag ggtgagccgg gcacgggcaa gaccttgctg gcccgcgaca tcgcccgctt
      421 cttcaatgct ccactggagc gctggcatgt caaatcgacg acgaaggcgg tcgacggact
      481 gtatcagtac gatgcggtcc ggcgcctgca cgacagccgc ttcggcgacg gcgaggtccg
      541 cgacattgcg cgttacatca cgctgggacc gctcgggcgg gcgtttcggc gcgacgagcg
      601 cgtggtcgtg ctcatcgacg agatcgacaa ggccgacctc gagttcccga acgatctact
      661 gcacgagctc gacaccatga gcttcacgat ctccgagacc ggcgagaccg tcgaggcgct
      721 tcaccggccg atcgtgatca tcaccagcaa caacgagaag gagctgccgg acgcgttcct
      781 gcgccgctgt gtgttccact ggatcgagtt ccccgaccgc gcgctgatgc agcggatcgt
      841 ccgggtgcac cacgccgacg ccgaggagca gctgctggcg agcgcgctcg aggcgttcta
      901 cacgctgcgc gccgtcgacg gcctgcgcaa acccccgagc accagcgagc tgatcgactg
      961 ggtgtcggcg ctgcggcaca gcggcatgga cccggccaag ctgctcggcg ccggcctgcc
     1021 cttcatcggc acgctgctca agaaggagca ggacatcgac accgcgcagc gccgcttcaa
     1081 cgcgccgcag gggacccggg gcggccgcac ggggcactga tgttcctcga cttcttttac
     1141 aagctgcgcg agcgcggcgt cccggtgacg acgcacacct ggctcgcgct gatcgaagcg
     1201 ctggcgaagg ggctgcatca ggactcgctc gacggctttt atcaagtcgc gcgctgcgtc
     1261 ctgatccaaa acgaggccca ctacgacgac ttcgaccgcg tgttcgcgga gagctttcgc
     1321 ggcgtggccg cggacgcgcg cgacctgctg tcggagatcg aaaagtggct cgcggacccg
     1381 aagctgctcg agcacctcga ccccgagctg cgcgcgcagc tcaagggcat ggacctcgag
     1441 gagctgcgca agcaattgct cgagcgcctg gagaagcaga aggagcgcca cgacggcggc
     1501 agccactggg tcggcacggg cggcacctcg ccgttcggca ccggcggcgc gcacccgacg
     1561 gggatccgcg tcggcggcgg tggtggccgc agcgcgctgg cggtcgccga cgcccggcgc
     1621 tttcgcgagt ttcgccgcga cctcgtgctc gacacccgcc agatcgcggc cgcgttacgc
     1681 cgcctgcgcc gcctgacccg cgacgacggc aagctggtcc tcgacctcga cgagacgatc
     1741 gacgcgaccg cccgcaactg cggcgacatc gaggtcgtgc tcaaccccga gcgccacgcc
     1801 gacgtgcgcc tgctgctgct gctcgacgtc ggcggcagca tggacccgca cgcgcagctg
     1861 gtctcgcgcc tgttctccgc ggcgcatcag ggcggcgggt tcaaggagct gcgctcgtac
     1921 tactttcata attgcgtgta ccagcgcgtg ttcgaggacg ccgcgttcac cagaccggta
     1981 cagaccgccg agctgatcaa gacgctcgac acgaagtgga ccgtcgtcat cgtcggcgac
     2041 gcgtacatgc acccgggcga gctgagcatg acgagcggcg actggtggga gagcacgcgc
     2101 gggccctcgg gtctgagctg gctggcgcgc ctcgccgacc gcttccctcg ca
//
LOCUS       DASWNJ010000072         3163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_177913, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000072 DASWNJ010000000
VERSION     DASWNJ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177913"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2989
                     /locus_tag="VGB85_01360"
     CDS             <1..2989
                     /locus_tag="VGB85_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEY0132685.1"
                     /translation="DPFGIIATQVAQRSWTVVEKNSLLVPLQDTLLYNRLLWLGIGLL
                     VLAGAYRMFRMEAAAGNRASKKRRKAQLDDAVEASAGPRPAGWSLPLVTPRASGPSRP
                     AALWAMVRLYFLNVTRDRSFRAIVAVGMLMTVLNAFNADKIFGTATYPVTYAMIEVIG
                     SFELFFLILTALYSGELVWRERSLRCDQILDATPVPTTLVFVAKILSLLAVHAVLVGF
                     LILAGVAVQAIKGYFLFELGVYFGYLYGFVYPAFALITLLAFFLQVVANHKFLGNGLV
                     VLFYILTMVLGAWGLDHRLYNYADAPRPTYSAMNGFGPNIPAYLWFSGYYLAFALILL
                     AIARLLLVRGTVTGWRSRLRVARSRITPAWLGFVAVATAAWIGLGAFIYHNTNVLNIY
                     RSRDDQDALTAEYERNYKQYEYLPQPRVIAIEVSVDIYPEKGHMRTTGTYKLVNRNEV
                     DVDQVHLLLNEAVTVHRLEFDRPSEVVSDDTRLRYQIRRLAQPLRPGEQMTLSFDVAY
                     DREGFGNRGPLTAIVDNGTFFNNGDFFPSIGYSADAELADDDRRKEQKLPPRERMPAI
                     DDERAWKNTYISHNADWIDFAATVCTSADQIAIAPGYLQREWTEGDRRCFAYKMDSKI
                     LPFYSFMSARYAVLRDEWRPAAGGDPVNIEIYYQPGHEYNLAAMVDGVKKSLDYFTTN
                     FSPYQHRQVRILEFPRYAQFAQAFPNTIPFSESIGFIARVDPEDPEDLDYPFYVTAHE
                     VAHQWWAHQVIGANVQGSTMLSESLSQYSALMVMEKKYGRDKMKRFLAYELRSYLRGR
                     GGERKKELPLALVENQQYIHYNKGSLVFYGLREYIGEDRLNAALARYIKQVAFQPPPF
                     TTTRDLLKILREVTPPAQQHLIEDMFETITLFDNKVVSASVEPRGDKFVVKLELATRK
                     LRADELGKESEIPIADEIEVGVFAAPEPGQELGRALHLERRPFNATTSTIEIVVDEAP
                     VRAGIDPYNKLIDRIPKDNTVPL"
BASE COUNT          526 a         1221 c          911 g          505 t
ORIGIN      
        1 cgacccgttc ggcatcatcg ccacccaggt cgcccagcgc agctggaccg tcgtcgagaa
       61 gaacagcctg ctcgtcccgc tccaggacac cctcctctac aaccgcctgc tgtggctcgg
      121 catcggcctg ctcgtgctcg ccggcgccta ccgcatgttc cgcatggagg ccgccgccgg
      181 caatcgtgcc agcaagaagc gccgcaaggc ccagctcgac gacgccgtcg aggcgagcgc
      241 cggcccgcgt cccgccggtt ggtcgctgcc cctcgtcacc ccacgcgctt cgggcccgag
      301 ccgcccggcc gcgctgtggg cgatggtccg cctgtacttc ctcaacgtca cgcgcgaccg
      361 ctcgttccgg gcgatcgtcg cggtcggcat gttgatgacg gtgctcaacg ccttcaacgc
      421 cgacaagatc ttcggcaccg cgacctaccc ggtcacctac gcgatgatcg aggtcatcgg
      481 cagcttcgag ctgttcttcc tgatcctgac cgccctctac agcggcgagc tggtgtggcg
      541 cgaacgcagc ctgcgctgcg accagatcct cgacgccacc cccgtgccga ccaccctcgt
      601 gttcgtcgcc aagatcctca gcctgctcgc cgtgcacgcc gtgctcgtcg gcttcctgat
      661 cctcgccggc gtcgccgtgc aggcgatcaa gggctacttc ctgttcgagc tcggggtcta
      721 cttcggttac ctctacggct tcgtctaccc cgccttcgcc ctgatcaccc tgctcgcgtt
      781 cttcttgcag gtcgtcgcca accacaagtt cctcggcaac ggcctcgtcg tcctgttcta
      841 catcctcacc atggtgctcg gcgcgtgggg cctcgaccat cgcctctaca actacgccga
      901 cgccccgcgg ccgacctact cggcgatgaa cggctttggc cccaacatcc ccgcgtacct
      961 gtggtttagc ggctactacc tcgcgttcgc cctgatcctg ctggcgatcg cccgcctgct
     1021 gctcgtgcgc ggcacggtca ccggctggcg cagtcgcctc cgcgtcgccc gttcacggat
     1081 caccccggcc tggctcggct tcgtcgccgt cgccaccgcc gcgtggatcg gcctcggcgc
     1141 gttcatctat cacaacacca acgtgctcaa catctaccgc agccgcgatg accaggacgc
     1201 gctgacggcc gagtacgagc gcaactacaa gcagtacgag tatctgccgc agccgcgcgt
     1261 gatcgcgatc gaggtctccg tcgacatcta ccccgagaag ggccacatgc ggacgaccgg
     1321 cacctacaag ctcgtcaacc gcaacgaggt cgacgtcgac caggtccacc tgctgctcaa
     1381 cgaggcggtc accgtgcatc gcctcgagtt cgaccgcccg tcggaggtcg tctccgacga
     1441 cacccgcctg cgctaccaga tccgccgcct cgcccagccg ctgcgccccg gcgagcagat
     1501 gacgctgagc ttcgacgtcg cctacgaccg cgagggcttc ggcaaccgcg gcccgctgac
     1561 cgcgatcgtc gacaacggca ctttctttaa taatggcgac ttcttcccga gcatcggcta
     1621 cagcgccgac gccgagctcg cggacgacga ccgccgcaag gagcagaagc tcccgccgcg
     1681 cgagcgcatg cccgcgatcg acgacgagcg cgcctggaag aacacctaca tctctcataa
     1741 cgccgactgg atcgacttcg ccgcgaccgt gtgcaccagc gccgaccaga tcgcgatcgc
     1801 ccccggctat ctgcagcgcg agtggaccga gggcgaccgc cgctgcttcg cctacaagat
     1861 ggacagcaag atcctcccgt tttactcgtt catgtcggcc cgctacgccg tcctgcgcga
     1921 cgagtggcgg ccggcggccg gcggcgaccc ggtcaacatc gagatctatt atcagcccgg
     1981 ccacgagtac aacctcgcgg cgatggtcga cggcgtcaag aagtcgctcg actacttcac
     2041 caccaacttt agcccgtacc agcaccgcca ggtccggatc ctcgagttcc cgcgctacgc
     2101 ccagttcgcg caggccttcc cgaacacgat cccgttctcg gagagcatcg gcttcatcgc
     2161 ccgcgtcgac cccgaggacc ccgaagacct cgactacccg ttctacgtca ccgcccacga
     2221 ggtcgcccac cagtggtggg cccaccaggt gatcggcgcc aacgtgcaag ggtcgaccat
     2281 gctctccgag tcgctgtcgc agtactcggc cctgatggtg atggagaaga agtacggccg
     2341 cgacaagatg aagcgcttcc tcgcctacga gctgcgcagc tatctccgcg gccgcggcgg
     2401 cgagcgcaaa aaagagctcc cgctcgcgct cgtcgagaac cagcagtaca tccactacaa
     2461 caagggctcg ctggtgttct acgggctgcg cgagtacatc ggcgaggacc gcctcaacgc
     2521 cgcgctcgct cgctacatca agcaggtcgc gttccagccc ccgcccttca cgaccacccg
     2581 cgacctgctc aagatcctgc gcgaggtgac ccctcccgcc cagcagcacc tgatcgagga
     2641 catgttcgag acgatcacgc tgttcgacaa caaggtcgtc agcgcctcgg tcgagccgcg
     2701 cggcgacaag ttcgtcgtca agctcgagct cgccacccgc aagctgcgcg ccgacgagct
     2761 cggcaaggag tcggagatcc cgatcgccga cgagatcgag gtcggcgtgt tcgcggcccc
     2821 cgaacccggt caggagctcg gccgggccct gcacctcgag cgccgcccct tcaacgccac
     2881 gaccagcacg atcgagatcg tcgtcgacga ggcccctgtg cgcgccggca tcgaccccta
     2941 caacaagctg atcgaccgca tcccgaagga caacaccgtc cccctgtgac ggctcaggcc
     3001 cgctccgggc ccaggcacgc ggcagcgaga cgatgccgcg tgccagcgag cagcgccgcg
     3061 aaggccgcgt cgttggtcgc caccaccgcg ccggcccccg cggccgcgcg ctcgtgcaac
     3121 agcgccgcca gcatcgccgc tccttcaggg tcgagcccgt tgc
//
LOCUS       DASWNJ010000073         4624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_425307, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000073 DASWNJ010000000
VERSION     DASWNJ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425307"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..980)
                     /gene="nudC"
                     /locus_tag="VGB85_01365"
     CDS             complement(114..980)
                     /gene="nudC"
                     /locus_tag="VGB85_01365"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEY0132686.1"
                     /translation="MTIGFTGSPLLRLDHERSDDAAFARHLADPQARLLRLDGLDPVT
                     SEAGGLAWGPLDERDPGAALALLGLLDGRPAFVALTPDPRGKPPFALLGLLAALPGPD
                     AAVWGMARSLVHWHATTRFCARCGASTEVTRAGWGRRCTACTTVVFPRTDPVVIMLAE
                     HGGRALIGRQPRYPAGRYSALAGFVEPGESVEEAVARELFEEAGVRATRVRYLASQPW
                     PLPSAQLMLACIAEVESDALTIDHDELDDAIWVDRAQVRAALAGDHDAPFIPPPPYAI
                     AHELLSRWSRGA"
     gene            complement(1041..3410)
                     /locus_tag="VGB85_01370"
     CDS             complement(1041..3410)
                     /locus_tag="VGB85_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0132687.1"
                     /translation="MLAPRVLIVHPPVSVARDYIDYPYFADLGAVQLASVLAQRWPTL
                     LVSAYALPDAGLVWRADGRARLGVDPEVLLAGVTGDLTAVVVAITPFHRPPRRDDLLA
                     AVLEDIRRRWPDTVVILAECYQSGQHHVTVAGVELLRSYPEADAFVQFEAEATVPELL
                     RRWIDHGERPRGAHRGEQPILDDLPPPAWAGLDLEALFRFHARVVAGLGRSAWAFPID
                     GRSLPLVTTRGCPFTCLHCSSNPDTPPGEPKRQRRRSPQQVSALLDTYKTLGATRIHV
                     LDELVNVHEAHFDHLLSELARLDLRVEIPNGMRADYLEPHHLAQLRGRITTVSVSAES
                     GVQRVVTEVVGKRLDLAAVVRVAEAAHAASVPLMIHYIIGMPGESAAEINATLAFALD
                     LWDRFHAWPAVQHATPLPGTRLHRLRSLPVLSDSDDGDWGPRFQRAASGVGEVAPELL
                     AKFMWTFEQRLRASQGPRKLVMNVTYVCNNHCTFCAVGTRTQLDGHPTRQREQLAHYR
                     AQGVTQVDFDGGEPTLNPELVPLVRHARALGYTRVNVTTNGRLAAYEAFAARLVNSGL
                     TSLLFSVHGPDARTHAQNVGVAEAFDQTVAGIRHCVRLAPAGLALGMNVTITKSNVDL
                     LGALAQLAWDLGLPWINLQFLTPFGRATRQVAPDTEHAARRAREVIDAWRARMKFQVI
                     NLPFCYMPDHAEFLQGDLGKLERHMVFVNNETVNLAQYLAERRTRKPQCAGCPHASFC
                     GGFYELDDVPEPPWLIAAEDLVRPIDDPRRHESVPAGFRGRMAARVRDS"
     gene            3464..>4624
                     /locus_tag="VGB85_01375"
     CDS             3464..>4624
                     /locus_tag="VGB85_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0132688.1"
                     /translation="MDTPGGTKILQHEEAAHELRNWVRLTFDCNDRCVFCLDAHTHDG
                     TVRAREEIKQQILDGRRKGAQRLILSGGEPTIHPDFIDFVRLGRLAGYRKVQTVTNGR
                     MFAYGEFLRRSLDAGLGEITFSIHGPNARIHDALVGTRGAFDEEVTGLKRALADGRPI
                     VNIDVCVNRGNVKQLPELLATFIAMGVREYDLLHVIPFGRAYTEGKDTLFYDLEEMRP
                     YLLAAFAYAREPGIHIWLNRFPPQHLEGFEDLIQDPYKLGDEVRGRKEEYAHLLERGV
                     PLDCRAPHRCGYCYLQPLCDTLDEVRARVEDAEFTVLRIDTTWEAQQAPVYGGDPASK
                     KRAETQIALRARHAGHSSGAEAEAAAAMAIEPVPGDSSKPVAPRLRLPLLGGL"
BASE COUNT          672 a         1508 c         1813 g          631 t
ORIGIN      
        1 gcatctgccc cggcgtgcgg cccgactacc tcgaggtgtt cggcgaccgc gagatcgcgg
       61 cggcgcgcgg gcaggcgccg acgatcgttc cacggcgctc gctgctgtga cgctcacgcg
      121 ccccgcgacc accgcgacag cagctcgtgg gcgatcgcgt agggcggcgg cgggatgaac
      181 ggcgcgtcgt ggtcgccggc cagcgccgcg cggacctgcg cgcggtcgac ccagatcgcg
      241 tcgtcgagct cgtcgtggtc gatcgtcagc gcgtcggact cgacctcggc gatgcacgcg
      301 agcatcagct gggcgctcgg gagaggccat ggctgactgg cgaggtagcg gacccgggtg
      361 gcccgtacgc ccgcctcctc gaacagctcg cgcgcgaccg cctcctcgac cgattcgccg
      421 ggctcgacga agcccgcgag cgcggagtac ctgcccgccg gataccgcgg ctggcgaccg
      481 atcagcgcgc gtccgccgtg ctccgccagc atgatgacca ccgggtcggt gcgtgggaac
      541 acgacggtgg tgcacgccgt gcagcgtcga ccccagcccg cgcgcgtgac ctcggtgctc
      601 gccccgcagc gcgcgcagaa gcgggtggtg gcgtgccagt ggaccaggct gcgcgccatg
      661 ccccagacgg cggcgtcggg gccgggcagc gcggcgagca gcccgagcag cgcgaacggc
      721 ggcttgccgc gcggatcggg ggtcagcgcg acgaaggcgg gccgcccatc gagcagcccg
      781 agcagcgcca gcgcggctcc cggatctcgc tcgtcgaggg gcccccaggc caggccgccc
      841 gcctcgctcg tgacggggtc gagcccgtcg agccgcagca ggcgcgcctg cggatcggcg
      901 aggtggcgag cgaaggccgc gtcgtcggat cgctcgtgat cgaggcgcag cagcggtgaa
      961 cccgtgaagc cgatcgtcat gcgcctccgg tgtgcgcgca acccgggcgc gaagcaagcg
     1021 gacgacgcgg gaccgggcga tcacgagtcg cgtacgcgcg cggccatgcg accgcgaaac
     1081 cctgcgggca cgctctcgtg tcgccgcggg tcgtcgatcg gccgcacgag gtcctccgcg
     1141 gcgatcagcc acggcggctc gggcacgtcg tcgagctcgt agaagccgcc acaaaagctc
     1201 gcgtgcggac atccggcgca ctgcggtttg cgggtgcgac gctcggcgag gtactgcgcg
     1261 aggttgacgg tctcgttgtt gacgaacacc atgtggcgct cgagcttgcc gaggtcgccc
     1321 tgcaggaact cggcgtgatc gggcatgtag cagaagggca ggttgatgac ctggaacttc
     1381 atgcgcgcgc gccaggcatc gatcacctcg cgcgcgcgcc gggccgcgtg ctcggtgtcg
     1441 ggcgcgacct gtcgcgtcgc gcggccgaag ggcgtcagga attgcaggtt gatccacggc
     1501 aggccgaggt cccaggcgag ctgggcgagc gcgccgagca gatcgacgtt ggacttcgtg
     1561 atcgtgacgt tcatgccgag cgcgaggccg gcgggggcga ggcgcacgca gtggcggatg
     1621 ccggcgaccg tctgatcgaa ggcctcggcg acgccgacgt tttgcgcgtg ggtgcgggcg
     1681 tcggggccgt gtacgctaaa cagcaggctc gtcaggccgc tgttgaccag gcgggcggcg
     1741 aaggcctcgt aggcggcgag gcggccgttg gtggtcacgt tgacgcgggt gtagccgagc
     1801 gcgcgggcgt ggcggaccag cgggaccagc tcggggttga gggtcggctc gccgccgtcg
     1861 aagtcgacct gcgtgacgcc ctgggcgcgg tagtgggcga gctgttcgcg ctggcgggtc
     1921 gggtggccgt cgagctgggt gcgggtgccg accgcacaga aggtgcaatg gttgttgcac
     1981 acgtaggtca cgttcatgac cagcttgcgc gggccctgcg aggcgcgcag gcgctgctcg
     2041 aaggtccaca tgaattttgc gagcagctcc ggtgcgacct cgccgacgcc gctggcggcg
     2101 cgctggaagc gggggcccca gtcgccgtcg tcgctgtccg aaaggacagg tagcgagcgc
     2161 aggcggtgca ggcgggtgcc gggcaggggg gtcgcgtgct gcaccgcggg ccaggcgtgg
     2221 aagcggtccc acaggtcgag ggcgaaggcg agggtcgcgt tgatctccgc ggcggactcg
     2281 ccgggcatgc cgatgatgta gtggatcatc agcggcacgc tcgccgcgtg ggcggcctcg
     2341 gcgacgcgca cgaccgcggc gaggtcgagg cgcttgccga cgacctcggt gacgacgcgc
     2401 tgcacgccgc tctcggcgct gacgctgacg gtcgtgatcc ggccgcgcag ctgcgcgagg
     2461 tggtgcggct cgaggtagtc ggcgcgcatg ccgttgggga tctcgacgcg caggtcgagg
     2521 cgggccagct cgctcaagag gtgatcgaag tgggcctcgt gcacgttgac cagctcgtcg
     2581 agcacgtgga tgcgcgtcgc gccgagggtt ttgtaagtgt cgagcagggc gctgacctgc
     2641 tgcggggacc ggcgtcgctg gcgtttgggc tcgccgggcg gcgtgtcggg gttcgagctg
     2701 cagtgcaggc aggtgaacgg gcagccgcgg gtggtgacga gcgggaggct gcggccgtcg
     2761 atcgggaagg cccaggcgct gcggccgagg ccggcgacca cgcgggcgtg gaagcggaat
     2821 aatgcctcga gatcgaggcc ggcccacgcg ggtggcggga gatcgtcgag gatcggctgc
     2881 tcgccgcggt gggcgccacg cggacgctcg ccgtggtcga tccagcggcg cagcagctcc
     2941 gggacggtgg cctcggcctc gaattgcacg aaggcgtcgg cctcggggta gctgcgcaga
     3001 agttccacgc cggcgacggt gacgtggtgc tggccgctct gataacactc ggcgaggatc
     3061 acgacggtgt cgggccagcg gcggcggatg tcctcgagga cagccgccag caggtcgtcg
     3121 cggcgcggtg gccggtgaaa cggcgtgatc gcgacgacca cggcggtgag gtcgccggtc
     3181 acgccggcca gcagcacctc gggatcgacg ccgaggcggg cgcggccgtc ggctcgccac
     3241 accaggcccg cgtccggcag ggcgtacgcg ctgaccagca gggtcggcca gcgctgtgcg
     3301 agcacggagg cgagttgtac tgcgccgagg tcggcgaagt agggatagtc gatgtagtcc
     3361 cgcgccaccg agaccggcgg atgcacgatc aggacgcggg gcgcgagcat cccgggcacg
     3421 atagcacgtg gcgcggcggc gcggcccgtg taggatgggc gcgatggaca cgcccggcgg
     3481 cacgaagatc ctccagcacg aggaggcggc gcacgagctt cgcaactggg tgcggttgac
     3541 cttcgactgc aacgaccgct gcgtgttctg tctcgacgcg cacacgcacg acggcacggt
     3601 gcgcgcgcgc gaggagatca agcaacagat cctcgacggg cggcgcaagg gggcccagcg
     3661 gctgatcctc agcgggggcg agccgacgat ccacccggac ttcatcgact tcgtgcggct
     3721 cgggcggctc gccgggtatc gcaaggtcca gacggtcacc aacgggcgga tgttcgcgta
     3781 cggagaattc ctgcggcgat cgctcgacgc cgggctcggc gagatcactt tttcgatcca
     3841 cgggcccaac gcgaggatcc acgacgcgct ggtcggcacg cgcggggcct tcgacgagga
     3901 ggtcaccggg ctcaagcggg cgctcgccga cgggcggccg atcgtcaaca tcgatgtttg
     3961 tgtcaatcgc gggaacgtca agcagctgcc cgagctgctg gcgaccttca tcgccatggg
     4021 cgtgcgtgag tacgacctgc tgcacgtgat cccgttcggg cgcgcctaca ccgagggcaa
     4081 ggacacgctg ttttatgacc tcgaggagat gcgaccgtat ctgctggcag cgttcgccta
     4141 tgcgcgcgag ccggggatcc acatctggct caatcgcttc ccgccgcagc acctcgaggg
     4201 ttttgaagac ctgatccagg acccctacaa gctcggcgac gaggtccgcg ggcgcaagga
     4261 ggagtacgcg cacctgctcg agcgcggcgt gccgctcgac tgccgcgcgc cgcatcgctg
     4321 cgggtactgc tatctgcaac cgctgtgcga cacgctcgac gaggtgcgcg cgcgggtcga
     4381 ggacgccgag ttcacggtgc tgcggatcga caccacgtgg gaggcccagc aggcgcccgt
     4441 gtacggcggc gatcctgcga gcaagaagcg ggccgagacg cagattgcgc tgcgggcccg
     4501 gcacgcgggg cacagctccg gcgcggaggc agaggcggcc gccgcgatgg cgatcgagcc
     4561 tgtccccggg gacagctcga agccggtcgc gccgcggctg cgcctgccgc tgctcggcgg
     4621 cctg
//
LOCUS       DASWNJ010000074         1657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_284058, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000074 DASWNJ010000000
VERSION     DASWNJ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284058"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="VGB85_01380"
     CDS             complement(<1..1163)
                     /locus_tag="VGB85_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132689.1"
                     /translation="MLAAFAQAPNYTRVPPELGTEVGWLCAGLLVLLLAWLATRMESV
                     RRSFLALEDPRMFAVMRIGVALMTIQNFWNLRPYWRMLWSDEGLYTVQEARQSMGGSA
                     LSGWTPVDGFLDSWAYLKFFWSKHSAFFFDAAPDHVETWMFVFFGVLLLYAAGVLSRV
                     TGVIAWLMLSGVYNHNALYLEGTDTVYRSFWFILLFCRTGAAWSVDNVVRCWWLRRRG
                     RLQEVGLPPSPGRSPVYKLVPSWPRYLMIGQLMAIYTSTGMVKTGSVWANGDALYYSL
                     NLDHFYRFELWTQMVSATFGTNLFRLMTWVTLWWESCFVVAGIGMILKFGHDHRDRAW
                     YQAQQVPWRLWLGRALLVIAYAVLYRLIILAYPYCVEFPKDASVEVQAKMVGEG"
     gene            1361..>1657
                     /locus_tag="VGB85_01385"
     CDS             1361..>1657
                     /locus_tag="VGB85_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0132690.1"
                     /translation="MGSVWTAIPVLHGFVCALALVSCARATLPPSAPGPALGRPLPEF
                     TRRSLDGATVSSGELRGKIVVVEFFAQYCKPCWKALPQVQKLAQGDPEVTVIGIG"
BASE COUNT          303 a          536 c          587 g          231 t
ORIGIN      
        1 ccctcgccga ccatcttcgc ctggacctcg acgctcgcgt ccttggggaa ctcgacgcag
       61 tacgggtacg cgaggatgat caggcggtag aggacggcgt aggcgatcac cagcagcgca
      121 cggccgagcc acaggcgcca cgggacctgc tgcgcctggt accaggcccg gtcgcggtga
      181 tcatggccga acttgaggat cattccgatg ccggcgacga cgaagcacga ctcccaccac
      241 agcgtcaccc aggtcatcag gcgaaacagg ttggtgccga aggtcgccga gaccatctgc
      301 gtccacagct cgaagcggta gaagtggtcg aggttgagcg agtagtagag cgcgtcgccg
      361 ttggcccaca cgctgccggt cttgaccatg ccggtgctgg tgtagatcgc catcagctgg
      421 ccgatcatca ggtagcgcgg ccagctcggg acgagcttgt agaccgggct gcggccgggc
      481 gagggcggga ggccgacctc ctgcaagcga ccgcggcgac ggagccacca gcagcgcacg
      541 acgttgtcga cgctccacgc ggcgccggtg cggcaaaaca gcaggatgaa ccagaacgag
      601 cgatagacgg tgtcggtgcc ctcgagatag agcgcgttgt ggttgtatac gcccgacagc
      661 atcagccagg cgatgacgcc ggtgacgcgg ctgagcacgc cggcggcgta cagcagcagc
      721 acgccgaaga acacgaacat ccaggtctcg acgtggtcgg gggcggcgtc gaagaagaag
      781 gcggagtgct tgctccaaaa gaacttgagg taagcccagg agtcgaggaa gccgtcgacg
      841 ggggtccagc cggacagggc ggagccgccc atgctctggc gggcctcctg cacggtgtag
      901 aggccctcgt cgctccacaa catgcgccag taggggcgca ggttccagaa gttctggatc
      961 gtcatcagcg cgacgccgat gcgcatcacc gcgaacatgc gcgggtcctc gagcgcgagg
     1021 aacgaccggc gcaccgactc catccgcgtc gccagccacg cgagcagcag caccagcagc
     1081 ccggcgcaca gccagccgac ctccgtgccg agctcgggtg gcacgcgggt gtagttcggg
     1141 gcttgtgcga aggcggcgag caaggtcggg ccagggggca agcgcggaca gtatcacgga
     1201 gcttcgcacg ggcctcgcga gcacgaaccg aaaactctgc gtgaacgcgc acacagcccc
     1261 cattggctgc cagccgcggg catttggccg tggtctctgg acggcacgta cgcgcgagct
     1321 gtgcgacact gcggcggtgc aaaggcccgg agtgcaagcg gtgggatcgg tgtggacggc
     1381 gatccccgtg ctccacgggt tcgtgtgcgc gctggcgctg gtctcgtgcg cccgggcgac
     1441 gctgccaccg tccgctccgg gcccggcgct cggccgtccg ctgccggagt tcacgcgaag
     1501 atctctcgac ggcgcgacgg tgtcctccgg cgagctgcgc ggcaagatcg tcgtcgtcga
     1561 gttcttcgcc cagtattgca aaccgtgctg gaaggccctg ccgcaggtcc aaaagctggc
     1621 ccagggcgac cccgaggtca ccgtgatcgg catcggc
//
LOCUS       DASWNJ010000075         2039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_318974, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000075 DASWNJ010000000
VERSION     DASWNJ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318974"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VGB85_01390"
     CDS             complement(<1..194)
                     /locus_tag="VGB85_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132691.1"
                     /translation="MNNITSMYRVFAAALLFTSLTACGDNGGGETSTTNPSTTTNPTA
                     SSTDDLTTSTTDPTSTTDPTS"
     gene            complement(205..897)
                     /locus_tag="VGB85_01395"
     CDS             complement(205..897)
                     /locus_tag="VGB85_01395"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HEY0132692.1"
                     /translation="MTPVATRDRPDLGDAMHKNMRSKTGLATIAIAAVCGGGWAASTV
                     FASDHDESPLVKLDAAQDITDLYVFESAPGKTTIIVCWAGFNDSRPQPDKAPLFDAKA
                     LYTIHVDNNGDNKADHQIYWRYGVNAQDEFGIQWEGIPGVDEAVSGPVETIFDASALA
                     RVWTGHADDPFFFDAQGYLETLDTGTVSMKSDRDFLKGLNVTAAAIEIDTDLLAGGPN
                     PIQVWATASRKK"
     gene            complement(1146..>2039)
                     /locus_tag="VGB85_01400"
     CDS             complement(1146..>2039)
                     /locus_tag="VGB85_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY0132693.1"
                     /translation="MNFALGDGLGGFTPGPGLEPGHSSPRGLVTAEVTGDDLPDVLVL
                     TDEDTMLLFATTGDGTPPPPQLLPLPEEWLRDLATGDLDGDGDTDLLAVGDSVVLLRN
                     DGAGKFAPESFATDGGMWRGGVVLHDLDGDGDLDAVVGRYANQGGAVEVLHNDGTGVM
                     TSVQQLDMVDGVTSLALLPTGPDTAPIVLITAGHAMQHAQVAGDGTLEAPQSFAGDTL
                     YEALTGRFDGDARPDAMLLEVGSLGALLGHDTWPAALVPVADVEISFWPTGTAIGDVN
                     GDGLDDIALSFFDLEVFVSNP"
BASE COUNT          299 a          660 c          728 g          352 t
ORIGIN      
        1 gacgtcgggt ccgtcgtcga ggtcgggtcc gtcgtcgagg tcgtcaggtc gtcggtcgag
       61 ctggccgtcg ggttggtggt ggtgctggga ttggtggtgc tggtttcgcc gccgccgttg
      121 tcaccacacg cggtgagcga cgtgaagagg agcgccgccg caaaaactcg gtacatggaa
      181 gtaatgttgt tcatggcggt ctccttactt cttcctcgag gccgtcgccc acacctggat
      241 gggattgggc ccgccggcca ggagatcggt gtcgatctcg atcgcggcgg cggtgacgtt
      301 gaggcccttg aggaagtcac ggtcgctctt catcgacacc gtcccggtgt cgagcgtctc
      361 taggtaaccc tgcgcgtcga agaagaacgg gtcgtccgcg tgaccggtcc acacgcgcgc
      421 gagggcgctc gcgtcgaaga tcgtctccac ggggcccgac acggcctcgt cgacgccggg
      481 gatgccctcc cactggatgc cgaactcgtc ctgcgcgttc acaccgtagc gccagtagat
      541 ctggtggtcg gccttgttgt cgccgttgtt gtcgacgtgg atcgtgtaca gcgccttggc
      601 gtcgaacagc ggcgccttgt ccggctgcgg tcggctgtcg ttgaagcccg cccagcacac
      661 gatgatggtc gtcttgccgg gggcgctctc gaagacatac aggtcggtga tgtcctgcgc
      721 cgcgtccagc ttcacgaggg gtgactcgtc gtggtcggat gcgaagacgg tggaggccgc
      781 ccagcctccc ccgcagacag cagcgatggc gatggtcgcc agcccagttt tcgatctcat
      841 gttcttgtgc atcgcgtctc ccaagtcagg tcggtcgcga gtcgcgaccg gagtcatgtc
      901 gagggatcgt acgcgggcgt cggtcctttg gatcgtgccg cgactcgcgc gaggcgaaat
      961 tctgcgtggc cgtcacgtac acggcgacgc aaacgcgctc gttcgccccg gatcggccgc
     1021 gaaatcgtgt acgcgcgcac tcgggagcac gccctcatac gcggcacgaa tgggggcaaa
     1081 acgcgttatt gcgtcaatac gagcgcgcga gcggggacat tcgaccactg tacgacgcga
     1141 cgcgctcacg ggtttgatac gaacacctcg aggtcgaaga acgagagggc gatgtcgtcg
     1201 aggccgtcgc cgttgacgtc accaatggca gtaccggtcg gccagaagct gatctccacg
     1261 tcggcgaccg gcaccagcgc cgccggccag gtgtcgtgtc cgagcagcgc gccgaggctc
     1321 cccacctcga gcagcatcgc gtcagggcgc gcgtcgccgt cgaagcggcc ggtcaaggcc
     1381 tcgtataagg tgtcgccggc gaagctctgc ggcgcctcga gggtgccgtc accggcgacc
     1441 tgcgcgtgct gcatcgcgtg gccggcggtg atcagcacga tcggcgcggt gtccgggccg
     1501 gtcgggagca gcgcgaggct cgtgacacca tcgaccatgt cgagctgctg gacgctggtc
     1561 atcacgccgg tcccgtcgtt gtgcaggacc tcgaccgcgc cgccctggtt cgcgtagcgc
     1621 ccgacgaccg cgtcgaggtc accgtcgccg tcgaggtcgt gcaggaccac gccaccgcgc
     1681 cacatgccac cgtcggtcgc gaagctctcg ggggcgaatt tgccggcgcc gtcgttgcgc
     1741 agcagcacga cgctgtcgcc gacggcgagc aggtcggtgt cgccgtcgcc gtcgaggtcg
     1801 ccggtggcga ggtcgcgcag ccactcctcg ggcagcggca gcagctgcgg cggcggcggc
     1861 gtgccatcgc cggtggttgc aaacagcagc atcgtgtcct cgtcggtgag caccaggacg
     1921 tccggcaggt cgtcgccggt gacctcggcg gtgaccaggc cgcgcgggct gctgtgcccg
     1981 ggctcgaggc cggggccggg cgtaaagccg ccgaggccgt cgccgagcgc gaagttcat
//
LOCUS       DASWNJ010000076         2585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_672388, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000076 DASWNJ010000000
VERSION     DASWNJ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672388"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VGB85_01405"
     CDS             complement(<1..912)
                     /locus_tag="VGB85_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132694.1"
                     /translation="MTCPMRHVLAAILALAPACERWRADPPEVPEHAVDFPRARASRV
                     AGVPVVRIAAPVGTAAVGVALRLGVAGDPPDRPGITALTLAALRGHGERPGGLAVTLG
                     EFGGVPELVIGREGALLTAEVPAQNAPAAVQALLAALRRPLTDPEELATIAAELASAR
                     ARARDDPGALALAALRRVAYPAGHPYRATQLSARTWSIEQVREHQARALGPAVLAVVV
                     AGEADDRALARAVEAGLAAWSTTVAPPVPPSIAAAPRHAVHLVARPGLAQAVVALGAA
                     TPTEDELAAEVADLLVQSALYDRLRGEL"
     gene            complement(909..2294)
                     /locus_tag="VGB85_01410"
     CDS             complement(909..2294)
                     /locus_tag="VGB85_01410"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0132695.1"
                     /translation="MPILAALLPLWLALAPAPGPTSAPVRRVLDNGLEVIVQRDARLP
                     LVAVHVRYHVGAVDDPPDRRGLAHVVEHFMFEGSRHIDAAAREEARGRAAIHHDNAET
                     DLHSSEYFTLAPRGSLASALWLESDRMGFLRGHAGVATLARVKAIVAQERRQRREADP
                     RGARIDRLFGELFPPEHPYHDTVMGPHAAIAAVTLADVDGFLARHYTPRNATLTIVGD
                     VPPNTDALIDRHFAGLDSPPAPSDSRDGRPPATPGADVPVLSASPPRPPPPARRPRTL
                     PPVADGPIRVVRLPGGPPGALCGWPSPGMHADGDAALDVRFTALTAGALVRLGGPDAA
                     AIADLHAQQVSQPGQSIALIELHGQAGADPGELHRALLRVLGRVAAGELDPAEVRRAR
                     LRLATGLRVATQNLLTRAALISSYATVFGDPDALARDLEQWLAVTPEAVAAAARTLLA
                     TRAAVVLSGPP"
BASE COUNT          322 a          883 c         1101 g          279 t
ORIGIN      
        1 cagctcgcca cgcagacggt cgtagagggc gctctggacc agcaggtcgg cgacctcggc
       61 cgccagctcg tcctcggtgg gggtcgccgc gccgagagcg accaccgcct gcgcgagccc
      121 gggtcgtgcg acgaggtgga ccgcgtggcg cggggccgcg gcaatcgacg gggggacggg
      181 cggggcgacg gtggtcgacc acgcggcgag gccggcctcg acggcgcggg cgagcgcgcg
      241 gtcgtcggcc tcgcccgcga cgacgaccgc cagcacggcg ggcccgagcg cgcgcgcctg
      301 gtgctcgcgg acctgttcga tcgaccatgt cctcgcggac agctgagtgg cgcggtaggg
      361 gtggcccgcc gggtaggcga cgcggcgcag cgcggcgagg gcgagcgcgc cggggtcgtc
      421 gcgggcgcgg gcgcgggcgg aggcgagctc ggcggcgatc gtggcgagct cctcggggtc
      481 ggtgaggggc cggcgcagcg cggcgagcaa cgcctgcacg gcggcggggg cgttctgcgc
      541 ggggacctcg gcggtcagca gggcgccctc gcggccgatc acgagctcgg ggacgccgcc
      601 gaattctccg agcgtgacgg cgaggccgcc ggggcgctcc ccatgaccgc gcagggccgc
      661 gagcgtcagc gcggtgatgc cgggccggtc cggcggatcg ccggcgacgc cgaggcgcag
      721 cgcgacgccg accgccgcgg tgccgaccgg cgcggcgatc cggaccacgg gcaccccggc
      781 gacccgggac gcgcgcgcgc gcgggaagtc gacggcgtgc tcgggcacct cgggcggatc
      841 ggcccgccag cgctcgcagg ccggggcgag cgcgaggatg gccgcgagga catgtcgcat
      901 cggacaggtc atggcggccc cgagagcacg accgcggcgc gggtggcgag aagcgtgcgc
      961 gcggcggcgg cgacggcctc gggcgtgacg gcgagccatt gctcgaggtc tcgcgcgagc
     1021 gcgtcggggt cgccgaagac agtcgcgtag ctcgagatca gggcggcgcg ggtcagcagg
     1081 ttctgggtgg cgacgcgcag gccggtggcg aggcgcaggc gggcgcgccg gacctcggcc
     1141 gggtcgagct cgccggcggc gacgcggccg agcacgcgca gcagggcgcg gtggagctcg
     1201 ccaggatcgg cgccggcctg gccgtgcagc tcgatcagcg cgatcgactg gccgggctgg
     1261 ctgacctgct gggcgtggag gtcggcgatc gcggcggcgt cgggtccgcc gaggcggacc
     1321 agagcgccag ccgtgagcgc ggtgaaccgc acgtcgaggg cggcgtcgcc gtcggcgtgc
     1381 atgcccggcg agggccagcc gcacagggca ccgggcgggc caccgggcag ccggacgacg
     1441 cgtatcgggc cgtcggcgac cggcggcaac gtacgcgggc gccgggcagg tgggggcggc
     1501 cgtggcgggc tggctgaaag gacaggtacg tcggcgcccg gggtcgcggg cggtcggccg
     1561 tcgcggctgt ccgaaggggc aggtggagaa tcgaggccgg cgaagtggcg atcgatcagc
     1621 gcgtcggtgt tcggcgggac gtcgccgacg atcgtcaggg tcgcgtttcg cggggtgtag
     1681 tggcgggcca ggaagccatc gacatcggcc agggtcacgg ccgcgatcgc ggcgtgcggg
     1741 cccatgacgg tgtcgtggta cgggtgctcg ggcgggaaca gctcgccgaa caggcgatcg
     1801 atgcgggcgc cgcgagggtc ggcctcgcgc cgctggcggc gctcctgggc gacgatcgcc
     1861 ttgacccgcg cgagcgtggc gacgccggcg tgcccgcgca aaaagcccat gcggtcgctc
     1921 tccagccaca gcgcgctcgc caggctgccg cgcggcgcga gcgtgaagta ctcgctgctg
     1981 tgcaggtcgg tctcggcgtt gtcgtggtgg atcgcggcgc gtccgcgcgc ctcctcgcgc
     2041 gcggcggcgt cgatgtgccg cgatccctcg aacatgaagt gctcgacgac gtgcgcgagg
     2101 ccgcggcggt cgggcgggtc gtcgacggcg ccgacgtgat accggacgtg cacggcgacc
     2161 agcggcaggc gggcgtcgcg ttgcacgatg acctcgaggc cgttgtcgag gacgcgccgc
     2221 acgggggccg aggtgggccc aggggcgggc gcgagggcca gccacagcgg cagcaaggcg
     2281 gcgaggatcg gcatctcatg acggtattca tggccgcgca ggcgaggtca agcgcgacgc
     2341 cgtccgggtc cgatcgcggg ccgaccgcac acgcgtgtcc cgcggatccg tgacgaagcc
     2401 gcggcggtgg gggtcccgct cccggagaat tttgctaata ctcgccctgc cgatccacgc
     2461 cgggtcggcc cgactcgcgg agcacgtctt gtcgacacct cctccccccc gggacagccc
     2521 gccacccgcc gcggatccag ccgcggatcc agccgcggat ccagccgcgg atccagccgc
     2581 ggatc
//
LOCUS       DASWNJ010000077         1614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_425459, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000077 DASWNJ010000000
VERSION     DASWNJ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425459"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VGB85_01415"
     CDS             complement(<1..542)
                     /locus_tag="VGB85_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132696.1"
                     /translation="MRSLIMLVAGSVAIVTVVAVLLRSDAGQTASSDASASQRGSQQP
                     LGLRGANIDSARRGATDEDSPSSMVSPGAGLAEEAAVAGKRERPEPEPPAPPIPPSAT
                     AVAEGEASLAAQPQVLAAVEQALSDQRSAIRRDCWNGTLPSSASFPVTASYGADGTML
                     ALSVGDDRGAPSVGSCVRSRP"
     gene            complement(592..897)
                     /locus_tag="VGB85_01420"
     CDS             complement(592..897)
                     /locus_tag="VGB85_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132697.1"
                     /translation="MTPVPPAASVVPRDTVTQQATEALAYQLGTLRARCPPPPGEHKF
                     TLNVTFDALGAEVTRGIAEMRTNPRSELGSCIAGALKPLRVAPPGAVTMVEVPLSLP"
     gene            complement(1068..1457)
                     /locus_tag="VGB85_01425"
     CDS             complement(1068..1457)
                     /locus_tag="VGB85_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132698.1"
                     /translation="MFNGFAGASIAGSAGAYVFVGPFAVVTTTVSQRLTAAGTAPIAL
                     TVPGPQTAQLGLCYQNNANPGVLTNFVGGAYSIVQFTNIRISQAVAASVVPGAGTWRV
                     GVCVQNNGGANALANNDYVNGWVQVTN"
BASE COUNT          247 a          590 c          545 g          232 t
ORIGIN      
        1 ggccgggagc gcacgcaact accgacgctg ggcgccccgc ggtcgtcgcc gacgctgagc
       61 gccaacatcg taccgtccgc gccgtagctc gccgtgaccg ggaagctcgc tgacgacggt
      121 agcgtaccgt tccagcagtc gcgccggatg gcggaccgct ggtcggagag cgcctgctca
      181 acggcagcca ggacctgcgg ctgagccgcc aggctcgcct caccctcggc gacggccgtc
      241 gcgctcggcg ggatcggcgg tgcgggcggc tccggttcag gccgctcgcg cttcccggcg
      301 accgcggctt cctccgcgag tcctgccccg ggcgacacca tcgacgacgg cgagtcttcg
      361 tccgtcgcgc ctcgccgcgc cgaatcgatg ttcgccccgc gcaacccgag cggttgctgt
      421 gaccctcgtt gcgacgccga tgcatcggat gacgccgtct ggccggcgtc ggaccgcagc
      481 agcaccgcga cgacggtgac gatcgccacg ctccccgcca ccaacatgat cagactgcgc
      541 atcgctatcg acctgctccc ttcatataca tataagcccc ggcggccagc ctcagggcag
      601 gctcagcggc acctcgacca tcgtcacggc gcccggcggt gccacgcgca gaggcttgag
      661 cgccccggcg atgcatgacc cgagctccga tcgtggattt gtacgcatct cggcgatacc
      721 gcgcgtcacc tcggccccga gcgcatcgaa cgttacattg agcgtgaact tgtgctcgcc
      781 cggcgggggc gggcagcgag cgcgcagcgt gccgagctgg tacgccagcg cctcggtcgc
      841 ttgctgcgtg accgtgtcgc gcgggaccac cgacgcggcc ggtgggacgg gcgtcaatgg
      901 cggtgtgacc gctgcggtcg gcgacgacgt gcggccaatt gcggcgaaaa cgccgaccgc
      961 gatgatcgca cttccgatca ggatcgcggc cggcgtcgag atgttcgccc ctgtgctcat
     1021 atgcgtgcgc cctgccacgc taccgtggca gggcgcactc tatcacatca gttcgtgacc
     1081 tggacccagc cgttcacgta gtcgttgttc gccagagcgt tggctccgcc gttgttttgc
     1141 acacacaccc cgacgcgcca ggtgcccgca ccaggaacga cgctcgcggc aacagcctgc
     1201 gagatgcgga tgttcgtgaa ctgcacgatc gagtaggcgc cgccgacgaa gttcgtgaga
     1261 actccggggt tggcgttatt ctggtagcag agccccagct gcgcggtctg aggacccggg
     1321 acggtgaggg cgatcggcgc cgtgccggcg gcggtcaagc gctgcgacac cgtggtcgtc
     1381 acgactgcga acggaccgac gaacacgtaa gcccctgcgc tacccgcgat cgaagcgcct
     1441 gcgaagccgt tgaacacacc ggtcgccacg atgccggtgg gacccgcagg acccgcagga
     1501 cccgcaggac ccgtgggacc ggcagggccc gtcgcgcccg caggaccggc cgggcccatc
     1561 ggaccgaccg cgcccgcagg gcccgtcgcg cccgcaggac ccgtcgcgcc catc
//
LOCUS       DASWNJ010000078         3643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_213208, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000078 DASWNJ010000000
VERSION     DASWNJ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213208"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VGB85_01430"
     CDS             <1..357
                     /locus_tag="VGB85_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY0132699.1"
                     /translation="PTSGLTRAEVEALASQAAAARQQDLARRAQVELRNRAETLAYAC
                     ERALAGYGEGLPAAARAEVSRDLEDLRGVLGGHAEPAALQPAFLALERSSRHIYAAML
                     ESSAQDEDQPQGTRSA"
     gene            354..1445
                     /locus_tag="VGB85_01435"
     CDS             354..1445
                     /locus_tag="VGB85_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY0132700.1"
                     /translation="MTYQDHYAVLGVPHSATAEEIKAAYRRLALATHPDRHPDDPDAE
                     ARFRAISSAYAVLSDPAQRSRYDTQRLLPDVFTNGQGQGVSLQTAKDLLAAVVGDVFG
                     RQRRERRRGRDIRYTLTVELAQAVLGGEHEIAFEALGTCSTCGGSGTRPGGRPAITCP
                     LCQGRGELKGEGFLRRYTACGRCGGVGMIQQDPCNPCSGRGSRREKRNFHVKLPPGTE
                     SGTERVVEGQGEPGQYGGSPGHLRVTVNVKPHPWLRRDGADIVGELHVSVVEAARGGR
                     IPVPTLTGTAIVDLPAGVATGTKLRLRGKGVPGEGTRPGDQIVTVMLETPKISGASSP
                     VIAALEALEKALDAEPDLLPKRSAQRKGP"
     gene            1498..1761
                     /locus_tag="VGB85_01440"
     CDS             1498..1761
                     /locus_tag="VGB85_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132701.1"
                     /translation="MASGDVQKQLQELEQLATQLGVRICYDAMTGLSQGSGGLCKVRG
                     EWRIIMDKRLKAVERLQVISDALRSRFDTEAHFVSPQVRSLLS"
     gene            complement(1816..2232)
                     /locus_tag="VGB85_01445"
     CDS             complement(1816..2232)
                     /locus_tag="VGB85_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132702.1"
                     /translation="MRDRHDDPSAQDPLPGLRGVGRSTPATGATHAKAVDGAAETSAA
                     TEVGAASSVGAASAAGGASEVGAVGAVAADPVASITDALATGAIDADTARSRLIDAVV
                     ASQMPPGADPAVWQAIRAEVEALVAGDPTIADLLRV"
     gene            2526..>3643
                     /locus_tag="VGB85_01450"
     CDS             2526..>3643
                     /locus_tag="VGB85_01450"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0132703.1"
                     /translation="MNQITQFGPYKLIERISVGGMAEVYKATEHGVEGFERTVAVKRI
                     LPHIAEDDEFITMFKDEAKIAGQLNHGNIAQIYNLGQQGDSFYIALEYVAGKDLRNIF
                     TRCQQQGRPMPIAQACFIVMKVSEGLDYAHNKRDKHGRHLNIVHRDVSPPNVLVSYEG
                     EVKLIDFGVAKAAGRVSRTQAGILKGKFGYMSPEQVRGMPLDRRSDVFSLGVVLYETL
                     VGQRLFQGDTDFATLEMVRTVDVPAPSSKNPEIPKDLEAILMKALSGEPETRYQTAME
                     LHDELQAFMFQHGMFYSRKDLAAWMRKQYAREIELEKDKSSGKPGGPPRPAPPGAAAG
                     RKPTLSNVPSPKAPPPNVPTGPSAQPRPPVVASKPPPGS"
BASE COUNT          561 a         1375 c         1235 g          472 t
ORIGIN      
        1 ccgacctcgg ggctgacccg cgccgaggtc gaggcgctcg cgtcgcaggc cgcggccgcc
       61 cgccagcagg acctcgcgcg ccgcgcccag gtcgagctgc gcaaccgcgc cgagacgctc
      121 gcctacgcgt gcgagcgggc gctcgccggc tacggcgagg gcttgccggc ggccgcccgc
      181 gccgaggtct cgcgcgacct ggaggacctg cgcggcgtgc tcggaggcca cgccgagccg
      241 gccgcgttgc agccggcatt cctggcgctc gagcgatcgt cgcgtcacat ctacgcggcg
      301 atgctcgagt cttccgcaca ggacgaggac caaccccagg ggacccgcag cgcatgacct
      361 accaggacca ctacgccgtc ctcggcgtgc cgcacagcgc gacggccgag gagatcaagg
      421 ccgcgtaccg ccgcctcgcc ctcgccacgc acccggaccg ccaccccgac gaccccgacg
      481 ccgaggcccg ctttcgcgcg atctccagcg cctacgcggt gctctcggac cccgcccagc
      541 gcagccgcta cgacacccag cgcctgctgc cggacgtgtt caccaatggg caagggcagg
      601 gcgtcagcct ccagaccgcc aaggatctgc tcgccgcggt cgtcggcgat gtcttcggcc
      661 gccagcgtcg cgagcgtcgc cgcggtcgcg acatccgcta caccctcacc gtcgagctcg
      721 cgcaggccgt gctcggcggc gagcacgaga tcgccttcga ggccctcggc acctgcagca
      781 cctgcggcgg ctccggcacc cgacccggcg gtcgcccggc gatcacctgt ccgctgtgcc
      841 agggccgcgg cgagctcaag ggcgagggct tcctgcgccg ctacaccgcc tgcggccgct
      901 gcggcggcgt cggcatgatc cagcaggacc cgtgcaaccc gtgcagcggc cgcggctcgc
      961 gtcgcgagaa gcgtaacttc cacgtcaagc tcccgccggg caccgaatcc ggcacggagc
     1021 gcgtcgtcga gggccagggc gagcccggcc agtacggcgg atctcccggg cacctgcgcg
     1081 tgacggtcaa cgtcaagccg cacccgtggc tgcgccgcga cggcgccgac atcgtcggcg
     1141 agctgcacgt cagcgtcgtc gaggccgcgc gcggcggccg gatccccgtg ccgaccctga
     1201 ccggcaccgc gatcgtcgac ctgcccgccg gcgtcgccac cggcaccaag ctgcgcctgc
     1261 gcggcaaggg cgtgcccggc gaggggacgc gccccggcga tcagatcgtc accgtcatgc
     1321 tcgagacgcc gaagatctcg ggcgcgagca gcccggtgat cgccgcgctc gaggctctgg
     1381 aaaaggccct cgatgccgag ccggatctcc tccccaaacg ctccgcccag cgcaagggac
     1441 cctgaataac actggccaag gcgtgggctt tacggcagcc tcgccgcgag gaacgggatg
     1501 gcgagcgggg acgtacaaaa gcagctgcaa gagctcgagc agctggcgac acagctcggc
     1561 gtgcgcatct gctacgacgc gatgaccggg ctcagccagg gcagcggcgg gctgtgcaag
     1621 gtccgcggcg agtggcgcat catcatggac aagcggctca aggcggtcga gcgcctgcag
     1681 gtcatctccg acgccctgcg ctcgcgcttc gacaccgagg cccacttcgt ttcgccgcag
     1741 gtgcgcagcc tgctgtcctg acctgacccc ggggacaggc cgctggcgag ggcgtgacct
     1801 gtcctctcgg tcaggtcaga cgcgcagcag gtcggcgatc gtcgggtccc ccgcgacgag
     1861 cgcctcgacc tcggcgcgga tcgcctgcca cacggcgggg tcggctcccg gcggcatctg
     1921 cgaggcgacg accgcgtcga tcagccggct gcgcgcggtg tcagcgtcga tcgcgcccgt
     1981 ggcgagggca tccgtgatcg aagcgaccgg gtcggcggcg accgctccga cggccccgac
     2041 ctcgctcgcc ccgccggctg ccgaggccgc gccaacagag gaggccgcgc cgacttcggt
     2101 ggccgcgctg gtctcggcgg cgccgtcgac cgccttggca tgcgtcgcgc cggtcgctgg
     2161 cgtcgagcgg cccacgccgc ggaggccggg caggggatct tgcgcgctgg ggtcgtcgtg
     2221 ccggtctcgc atgggcgcct cgctctctga ccttagcatc ggcgccaggc ccgggatcgt
     2281 tgcgcatgct cgccaaaaaa ctgcgcgcgc gccggccacg aattgggccg gtccggaccg
     2341 tcgatcgggc tcgattgagg gcatgcctgg ccggcgccga tcgaccgggc ttcccgcgcg
     2401 gtcagacccg atgcgcggtc agtccccgcc agggcccggg gaccgaggtg gacattgact
     2461 tttgccgggc ttcgggctca catgttgcag ccctgcgccc acgtgcggac gggtcggagt
     2521 tcctgttgaa tcagatcacc caattcggcc cttacaagct gatcgagcgc atcagcgtgg
     2581 gtggcatggc cgaggtgtac aaggccaccg agcacggcgt cgagggcttc gaacgtaccg
     2641 tggccgtcaa gcggatcttg ccccacatcg cggaggatga cgagttcatc acgatgttca
     2701 aggacgaggc caagatcgcc ggccagctca accacgggaa catcgctcag atctacaacc
     2761 tgggccagca aggcgacagc ttctacatcg ccctcgagta cgtcgccggc aaggacctgc
     2821 gcaacatctt cacgcgctgt cagcagcagg gccggccgat gccgatcgcc caggcctgct
     2881 tcatcgtcat gaaggtctcc gagggcctcg actacgcgca caacaagcgc gacaagcacg
     2941 gccgccacct caacatcgtc caccgcgacg tcagcccgcc caacgtgctc gtctcgtacg
     3001 agggcgaggt caagctgatc gacttcggcg tcgccaaggc cgccggtcgc gtgtcgcgga
     3061 cccaggccgg catcctcaag ggcaagttcg gctacatgtc cccggagcag gtccgcggca
     3121 tgccgctcga ccgccgcagc gacgtgttct cgctcggcgt cgtcctctac gagacgctcg
     3181 tcggccagcg cctgttccag ggcgacaccg acttcgcgac cctcgagatg gtgcgcaccg
     3241 tcgacgtgcc cgccccgagc agcaagaacc ccgagatccc caaggacctc gaggcgatcc
     3301 tgatgaaggc gctctcgggc gagcccgaga cccgctacca gacggcgatg gagctccacg
     3361 acgagctcca ggccttcatg ttccagcacg gcatgttcta cagccgcaag gacctcgcgg
     3421 cgtggatgcg aaagcagtac gcccgcgaga tcgagctcga gaaggacaag agctccggca
     3481 agcccggcgg gcccccgcgg cccgcacctc ccggcgccgc cgcgggccgc aagccgacgc
     3541 tgtcgaacgt gccctcgccc aaggccccgc cgccgaacgt gccgacgggt ccgagcgccc
     3601 agccgcgccc gccggtcgtc gcctcgaagc cgccgcccgg atc
//
LOCUS       DASWNJ010000079         5010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_178028, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000079 DASWNJ010000000
VERSION     DASWNJ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178028"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="VGB85_01455"
     CDS             <1..1135
                     /locus_tag="VGB85_01455"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0132704.1"
                     /translation="VHLKASTAAEARQTWQAALALRDDRGHESTLLSLTLLNNLARVA
                     QHDNDIDSQVGLHRQLVARSAQVLGEHHPWHLTYTATLAWTLARAGRPGEARRLLQRA
                     EPALARDESGQVRGWVMLVHGDLARGRVDHEDRDLVAARQSYQAVLDDRSASASDLYG
                     ARMGLGFVAAQEGREAEMHALLGQVVAETPYENGAIILAEALIDLGRPNEAVDELGAV
                     LARCAALDPAAPEAQGCHWSRYQLGRAYQQLGEHAAAERVLAQQAAIWEPVLAPHDLG
                     LLAVRRTLGEAVLDQGRFDEAAAILRAVEATYAAAVEPDYVPLAHTRLALARALTGER
                     EAMPADAAALAEQGLAALRAHGATFADELQAQENRVAGRSTGD"
     gene            1194..2765
                     /locus_tag="VGB85_01460"
     CDS             1194..2765
                     /locus_tag="VGB85_01460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEY0132705.1"
                     /translation="MLNVDDLPALERALAVCHAQRRTAEEGQQRLQMFLDGVEDYAFI
                     SFDLDGRIVGWSRGAEHILGYREVDALGLPSSIFFTPEDVLRGEDEREFLVAREAGRA
                     EDERWHLRRDGVRFWGSGVMTRRLDADGRLCGYAKVMRDHTARRLAEGRLRDSEERLR
                     LFSENVRDYALVPVDLEGLVVGWNSGAAGIFGYTSEEILGQHCARFFTPEDIAGDEPQ
                     RDLYLAATEGRSEYERWMVRADGSRFWAHWVTTPIHDDDDRLRGFAKVLHDGTDRKLA
                     QELRERLQAQEQVSLRTQVRSTGEALDRTNDELRALSASLLRAQEDERRHIARELHDD
                     LSQRLAVLAYKLAALHARAPALPAGVRGDVQRVETDVSVIAGEVRKLSHRLHPAILDD
                     LGLAVALRRLVEEFDAGRSEPTTFIEHGVPETVPHDASATFYRIAQEALRNISKHANT
                     GPVRVELDGAADKLCLTISDFGPGFDVVEARARGGLGIISMYERARLIGASLRLDSRP
                     GEGTVLTLSVPVADA"
     gene            complement(2775..3251)
                     /locus_tag="VGB85_01465"
     CDS             complement(2775..3251)
                     /locus_tag="VGB85_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132706.1"
                     /translation="MRSKDDQQQPDADSEAAGQGTRVSGGSGPGKDPTDTHAQEGDAS
                     GRMSSGSPREADVDTGVAADTGNTGGTGGAHGASTDNGGNAGVTVGNGGGPSEGTLNG
                     EPPPRQTSDLIGTGGTLDDRPSSGTGNTGVNGGGADPDAVSVGSASDRGIPGARKA"
     gene            complement(3425..>5010)
                     /locus_tag="VGB85_01470"
     CDS             complement(3425..>5010)
                     /locus_tag="VGB85_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132707.1"
                     /translation="GMAITADGNTLYTAVIGNDKVVAYDTGMLEDDSLYPDAADQIAV
                     SGGGPTGLVLDAPRDRLYVLTRFDNGISIIDTDTREEVDHLTMHNPEPAAVVAGRRFL
                     YDAALTSSRGDSSCASCHIFGDKDELAWDLGDPDGDVTGVPGVLRADFGIGQTSFHPM
                     KGPMTTQSLRGLDNHGVMHWRGDRNGEGQGQNVQPNGGAYSEVAAFTAFNPAFVGLLG
                     RDEQLTPAQMQAFTDFTLQVMYPPNPIRNLDNSLTADQQAGRDFFMNNKSIQIGEVFT
                     CVSCHTLNPTGNAQYGVAHPGFFGTEGKIITAEFSQTFKIPHLRNMYTKIGAFGFAED
                     PNFNVQGMPGYDTSFTGDQVRAFGFTHDGSKDTAMRFFNAFTKPFAPEGFTSFAQQKQ
                     VAEFVYAFDTNLKPIVGQQITLTANNNAVVGPRIDLLRNRAIAGDCELIVKASSSGYE
                     TGFWFTNGSYTSSFAAAPAVTDAQLRQTAQQFNVPLTYTCVPPGSGKRLGVDRDADGH
                     RDGDELLAGSDPADASSTP"
BASE COUNT          678 a         1756 c         1855 g          721 t
ORIGIN      
        1 cgtgcatttg aaagcctcga ccgccgccga ggccaggcag acctggcagg ccgcgctggc
       61 cctgcgcgac gaccgggggc acgagagcac gctgctgagc ctcaccctcc tcaacaacct
      121 cgccagggtc gcgcagcatg acaacgacat cgactcgcag gtcgggttgc accggcagct
      181 cgtcgccagg tcggcgcagg tgctcggcga gcaccacccc tggcacctga cctacacggc
      241 gacgctggcc tggacgctcg cgcgggccgg gcggcccggg gaggcgcggc gcctgttgca
      301 gcgcgccgag ccggcgctcg cgcgcgacga gagcgggcag gtccgcgggt gggtgatgct
      361 cgtgcacggt gacctcgcgc gcggcagggt cgaccacgag gaccgcgacc tcgtcgcggc
      421 gcgccagtcc taccaggcgg tgctcgacga ccgctcggcc agcgcgagcg acctgtacgg
      481 cgcgcggatg ggcctcggct tcgtcgccgc gcaggagggc cgcgaggccg agatgcacgc
      541 gctgctcggg caggtcgtcg ccgagacgcc gtacgagaac ggggcgatca tcctcgccga
      601 ggcgctgatc gacctcggac gcccgaacga ggcggtcgac gagctggggg ccgtgctggc
      661 gcgctgcgcc gcgctcgacc cggcggcgcc ggaggcccag ggctgccact ggagccgtta
      721 ccagctcggg cgcgcgtacc agcagctcgg cgagcacgcg gcggccgagc gggtgctcgc
      781 gcagcaggcc gcgatctggg agccggtgct agccccgcac gacctgggcc tgctcgccgt
      841 gcgccgcacg ctcggcgagg ccgtgctcga ccagggtcgc ttcgacgagg ccgccgcgat
      901 cctgcgcgcg gtcgaggcga cctacgcggc ggcggtcgag cccgactatg tgccgctggc
      961 tcacacgcgc ctcgctctcg cgcgggcgct gaccggcgag cgcgaggcga tgcccgccga
     1021 cgccgcggcg ctcgccgagc aggggctggc ggccctgcgg gcccacgggg cgaccttcgc
     1081 cgacgagctg caggcgcaag agaaccgcgt cgcgggccgt agtacgggcg attaatcgtc
     1141 cggccgggcc cgccgacacg ggcgcggcgg gtcgccgcga tgcactgtgt cgcatgctga
     1201 acgtcgacga cctccccgca ctcgagcgcg cgctcgcggt ctgtcatgcg cagcggcgga
     1261 ccgccgagga gggccagcag cggctgcaga tgttcctcga cggcgtcgag gactacgcgt
     1321 tcatcagctt cgacctcgac gggcggatcg tcggctggag ccgcggggcc gagcacatcc
     1381 tcggttatcg cgaggtcgac gcgctcggcc tgccgagcag catcttcttc acgccggagg
     1441 acgtgctgcg cggcgaggac gagcgcgagt tcctggtcgc gcgcgaggcg ggccgcgccg
     1501 aggacgagcg ctggcacctg cgacgcgacg gcgtccgctt ctggggcagc ggcgtgatga
     1561 cccggcggct cgacgccgac gggcggctgt gcggctacgc caaggtgatg cgcgaccaca
     1621 cggcgcgccg gctcgcggag ggccgcctgc gcgacagcga ggagcggctg cgcctgttct
     1681 cggagaacgt ccgcgactac gcgctggtcc cggtcgacct cgagggtctg gtggtcggct
     1741 ggaactccgg ggccgccggc atcttcggat ataccagcga ggagatcctc gggcagcact
     1801 gcgcccgctt cttcacgccc gaggacatcg ccggcgacga gccgcagcgt gacctgtacc
     1861 tggcggccac cgagggccgc tccgagtacg agcgctggat ggtgcgcgcc gacggcagcc
     1921 gcttctgggc ccactgggtc accacgccga tccacgacga cgacgatcgg ctgcgcggct
     1981 tcgccaaggt gctgcacgac ggcaccgacc gcaagctcgc ccaggagctg cgcgagcggc
     2041 tgcaggcgca ggagcaggtc tcgctgcgca cgcaggtgcg ctcgaccggc gaggccctcg
     2101 accgcacgaa cgacgagctg cgggcgctca gcgccagcct gctgcgcgcg caggaggacg
     2161 agcggcggca catcgcccgc gagctgcacg acgacctgtc ccagcgcctc gcggtcctcg
     2221 cctacaagct cgcggcgctg cacgccaggg cccccgcgct gcccgcgggc gtccgcggtg
     2281 atgtccagcg cgtcgagacg gatgtcagcg tgatcgccgg cgaggtgcgc aagctgtccc
     2341 acaggctgca ccccgcgatc ctcgacgacc tcgggctcgc cgtcgccctg cggcggctcg
     2401 tcgaggagtt cgacgccggg cgcagcgagc cgacgacctt catcgaacac ggcgtccccg
     2461 agaccgtccc gcacgacgcg agcgcgacgt tttatcgcat cgcccaggag gcgctgcgca
     2521 acatcagcaa gcacgccaac acgggaccgg tgcgcgtcga gctcgacggc gcggcggaca
     2581 aattgtgcct gacgatctcc gactttggac ccggcttcga cgtcgtcgag gcgcgcgccc
     2641 gcggcggcct cggcatcatc agcatgtacg agcgcgcgcg cctgatcggc gccagcctgc
     2701 gcctcgacag ccgaccaggc gagggcaccg tgctgacgct gtccgtcccg gtggccgacg
     2761 cgtgaacgtc ggcctcaggc cttgcgcgcg cccgggatgc cgcggtcgga cgccgagccg
     2821 acgctgaccg cgtccgggtc ggcgccgccg ccgttgaccc cggtgttgcc ggtgccgctc
     2881 gacggtcgat cgtcgagcgt gccgccggtg ccgatcaggt cgctggtctg ccgcggcggg
     2941 ggctcgccgt tcaacgtacc ctcgctcggc ccgccgccgt tgccgaccgt caccccggcg
     3001 ttgccgccgt tgtcggtcga cgcgccgtgg gctccgccgg tcccgccggt gttcccggtg
     3061 tccgccgcga cgcccgtgtc gacgtccgcc tcgcgcggcg agcccgagct catgcgcccc
     3121 gaggcgtcgc cctcctgcgc gtgcgtgtcg gtcggatcct tgccgggccc cgacccgccg
     3181 gagacgcggg tgccctgacc tgcggcttca gagtcggcgt cgggctgctg ctgatcgtct
     3241 ttcgagcgca tacgtatcct ccagagttgc ggtcgtgtgc cgacaaccct ggaggcaaag
     3301 cgcgggcgag ccaggccggg gcgggacggg ccgcgacgat ccgtgctgca cgccccgctc
     3361 gcgcggcgcg ccgcgaaccg gcgaggccgg cgcccgctga tcaagtgatc aagtgatcat
     3421 gggatcacgg cgtgctgctg gcgtcggccg ggtcggagcc ggcgagcagc tcgtcgccgt
     3481 cgcgatggcc gtcggcgtcg cggtcgacgc cgaggcgctt gcccgagccc ggcgggacgc
     3541 aggtgtaggt cagcggcacg ttgaactgct gcgccgtctg ccgcagctgg gcgtcggtga
     3601 ccgccggggc ggcggcgaag ctcgaggtgt agctgccgtt ggtgaaccaa aagccggtct
     3661 cgtagcccga cgagctggcc ttgacgatca gctcgcagtc gcccgcgatc gcgcggttgc
     3721 gcagcaggtc gatgcgcggg ccgacgaccg cgttgttgtt ggccgtcagc gtgatctgct
     3781 ggccgacgat cggcttgagg ttggtgtcga aggcgtagac gaactcggcg acctgcttct
     3841 gctgcgcgaa gctggtgaag ccctcggggg cgaacggctt ggtgaacgcg ttgaagaagc
     3901 gcatcgccgt gtccttggag ccgtcgtgcg tgaagccgaa cgcgcgcacc tggtcgcccg
     3961 tgaacgaggt gtcatagccc ggcataccct ggacattgaa gttcgggtcc tcggcgaagc
     4021 cgaacgcgcc gatcttggtg tacatattgc gcaggtgcgg gatcttgaag gtctgcgaga
     4081 actccgcggt gatgatcttg ccctcggtgc cgaagaagcc ggggtgcgcg acgccgtact
     4141 gcgcgttgcc ggtggggttg agggtgtgac agctgacgca ggtgaacacc tcgccgatct
     4201 ggatcgactt gttgttcatg aagaagtcgc gcccggcctg ctgatccgcc gtcaatgaat
     4261 tgtcgaggtt gcggatcggg ttcggcggat acatcacctg cagcgtgaag tccgtgaacg
     4321 cctgcatctg ggccggcgtc agctgctcgt cgcgcccgag caggccgacg aacgccgggt
     4381 tgaacgccgt gaacgcggcc acctcgctgt aggccccgcc gttgggctgg acattttgcc
     4441 cctgcccctc gccgttgcga tcgccgcgcc agtgcatgac gccgtggttg tccaggccgc
     4501 gcaggctctg ggtcgtcatc gggcccttca tcgggtgaaa cgaggtctgg ccgatgccga
     4561 agtcggcacg cagcacgccg ggcacccccg tcacgtcgcc gtccgggtcg ccgaggtccc
     4621 acgcgagctc gtccttgtcg ccgaagatgt gacagctcgc gcacgaggag tcgccgcggc
     4681 tcgaggtcag ggcggcgtcg tacaggaagc gccggcccgc gacgaccgcc gccggctcgg
     4741 ggttgtgcat cgtcaggtgg tcgacctcct cgcgcgtatc ggtgtcgatg atcgagatgc
     4801 cgttgtcgaa gcgggtcagc acatacaggc ggtcgcgcgg ggcgtcgagc accaggccgg
     4861 tcggaccgcc gccggacacg gcgatctggt cggccgcgtc ggggtagagg ctgtcgtcct
     4921 cgagcatgcc ggtgtcgtag gcgacgacct tgtcgttgcc gatcaccgcg gtgtacagcg
     4981 tgttgccgtc ggcggtgatc gccatgccga
//
LOCUS       DASWNJ010000080         2192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_36841, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000080 DASWNJ010000000
VERSION     DASWNJ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36841"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VGB85_01475"
     CDS             complement(<1..295)
                     /locus_tag="VGB85_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132708.1"
                     /translation="MTTPTTSTLRSPRHPRWADVPEDSWSDWRWQMRNRVTTLAALEE
                     LLALTDDERAGITARADAFPFAITPYYLSLIEGPGCPVRRQAVPHIDETITTPW"
     gene            complement(292..828)
                     /locus_tag="VGB85_01480"
     CDS             complement(292..828)
                     /locus_tag="VGB85_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132709.1"
                     /translation="MPESQPSVARLRSFLEEADIPSSVLRRDPEGTTWLPAELRALVT
                     ADEQCHSELRRFVDREIELFGSVRQKSDALFTDRVLKAAAPVQIVGAGLDSRQRGWIL
                     AFAYAMATVVAYLMLAPLLGLAALGTWSDRFATAVGVDETGGGVVGVRAALVLIGAAV
                     LVVAVAFMPSRRHTTPPA"
     gene            complement(821..1504)
                     /locus_tag="VGB85_01485"
     CDS             complement(821..1504)
                     /locus_tag="VGB85_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0132710.1"
                     /translation="MSKLNAASALTTSALTTSALAWFGFGRTSKAPSLVDCTDEDLVA
                     RAGQAQGEKAFAELLRRHQGKVRGLLLRLAGDRTLADDLAQEVFLRAYRGLAGFEGRA
                     RFSTWLYRIAYNVYLNHRTRVRELAALPEGFESGAMAPETSLSANRSDLRRDLDVAIG
                     ALPDRYRAVVMLYYLEDVSYPEIAEILDLPLGTVKTHLHRAKRILRENLHGWGDNDGP
                     ATHNEAEDA"
     gene            complement(1501..2064)
                     /locus_tag="VGB85_01490"
     CDS             complement(1501..2064)
                     /locus_tag="VGB85_01490"
                     /inference="COORDINATES: protein motif:HMM:NF039701.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HEY0132711.1"
                     /translation="MVAASLGVSSEAHAALPNLALNLDYWGGLHVTDLVGNVAGSIRG
                     CLSDAGLLGSFWGGVDPTVASATCAGGGSLVLDVAFLALLVIAVIAVVRIRQTSEQNR
                     LELARQYIEKGMNPPPTLFPSAAGNDLRRGIVLVFTGLGLLGASLVASGWSHGVQGLG
                     PAGLIPGFIGLGYLVSYACAVRGKKSS"
BASE COUNT          363 a          757 c          782 g          290 t
ORIGIN      
        1 cccacggggt cgtgatcgtc tcgtcgatgt gcgggaccgc ctggcgccgc acggggcatc
       61 cggggccctc gatcagcgag aggtagtacg gcgtgatcgc gaacgggaag gcgtcggcgc
      121 gcgcggtgat cccggcgcgc tcgtcgtccg tcagcgcgag caactcctcg agcgccgcga
      181 gcgtggtgac gcggttgcgc atctgccagc gccagtcgga ccagctgtcc tcggggacat
      241 cggcccagcg cgggtgccgg ggcgagcgaa gggtcgaggt cgtcggggtc gtcatgcggg
      301 cggcgtagta tgccgccgcg agggcatgaa cgcgaccgcg accacgagga ccgccgcgcc
      361 gatcagcacc agtgcggcgc gcacgccgac caccccgccg ccggtctcgt cgacgccgac
      421 cgcggtggcg aagcgatccg accaggtgcc gagcgcggcg aggccgagca gcggcgcgag
      481 catcaggtag gcgacgacgg tcgccatcgc ataggcgaac gcgaggatcc agccgcgttg
      541 ccggctgtcg aggccggcgc cgacgatctg gaccggcgct gccgccttga gcacgcggtc
      601 ggtgaacagc gcgtcgcttt tttgccgcac gctgccaaac agctcgatct cccggtcgac
      661 gaagcgccgc agctcgctgt ggcactgctc gtcggccgtg accagcgcgc gcagctcggc
      721 cggcagccag gtcgtgccct cggggtcacg ccgcagcacg ctcgagggga tgtcggcctc
      781 ctcgaggaaa ctgcgcaggc gcgcgaccga gggctgcgac tcaggcatcc tcggcctcgt
      841 tgtgcgtggc gggtccgtcg ttgtcacccc agccatgcag gttctcccgc aagatccgct
      901 tggcgcggtg caggtgggtc ttcacggtgc cgagcggcag gtcgaggatc tcggcgatct
      961 cggggtacga gacgtcctcg aggtaataca gcatgacgac cgcacggtag cgatccggca
     1021 gcgcaccgat cgcgacgtcg aggtcgcgtc gcaggtccga acggttggcg ctcagcgagg
     1081 tctccggggc catcgcgccc gactcgaagc cctccggcag cgcggccagc tcgcgcaccc
     1141 gcgtgcggtg gttgaggtag acgttgtagg cgatccggta cagccaggtc gagaaccggg
     1201 cgcgcccctc gaagccggcc aggccacgat aggcgcgcag gaacacctcc tgcgcgaggt
     1261 cgtcggcgag ggtgcggtca cccgcgaggc gcagcaagag cccgcggacc ttgccctgat
     1321 gccggcgcag cagctcggcg aaggccttct cgccctgggc ctgccccgca cgcgcgacca
     1381 ggtcctcgtc ggtacagtcg acgagcgacg gcgccttgct ggtgcgtccg aagccgaacc
     1441 acgccagcgc tgaggtcgtc agtgccgagg tggtcagggc cgatgcagcg ttcaacttgc
     1501 tcatgagctc ttcttccccc gcacggcgca cgcgtacgag accaggtagc cgaggccgat
     1561 aaacccgggg atcagccccg cgggtccgag accttgcacg ccgtgcgacc acccgctcgc
     1621 aacgagcgag gcgcccaaca gaccgaggcc ggtgaacacc agcacgatgc cacggcgaag
     1681 gtcattgccg gccgccgagg ggaacagcgt cggcggagga ttcattccct tttcgatgta
     1741 ctggcgagcc agctcgagcc gattttgttc gctggtctgg cgaatgcgga ccaccgcgat
     1801 cacggcgatc accagcagcg ccaggaaggc gacatcgagc accagcgacc cgccgccggc
     1861 gcacgtggcc gacgcgaccg tcgggtcgac cccgccccag aacgagccga gcagcccggc
     1921 atccgaaagg cagccgcgga tcgatccagc gacgttgccg acgaggtccg tgacatgcaa
     1981 cccgccccag tagtcgaggt tgagcgccaa gttcggcagc gccgcgtgag cctcggacga
     2041 gacccccagc gacgccgcga ccacgacaac cccgacgggc ttcttcttat cctgcaaaaa
     2101 agagcggggg gcggggcgag acaacgtgag aagtccgggc atgagggcgc gccagcgtgg
     2161 gacccgacga gtgtcctcgc ggtttcacag ac
//
LOCUS       DASWNJ010000081         4314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_1566, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000081 DASWNJ010000000
VERSION     DASWNJ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1566"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1551
                     /locus_tag="VGB85_01495"
     CDS             169..1551
                     /locus_tag="VGB85_01495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0132712.1"
                     /translation="MPAHGSLTTALDDLRWLRRLAFTLARDADEADDLVQDTLVTAWR
                     EPPRDLTGPARPWLSTVLRNLWRMRRRSGARRERREQDAAVSGTPTQPEHELVRLEVL
                     RTLVAALDRLDPGDRKIIVRRYFEGESAAEIARALAIPAATVRSRIHRSLKVLRAALD
                     EKFDARSTWSAAILAGPPIFPTSPPKGDDMSIFIKTVLGAGIGAAGCVGWIGMSAQPE
                     STIDPIESVEPVTTDPKMAWEQRRQGIRRVLPPLAIPTARDAGDREQREHDDFRELVR
                     ACLEDLESDASGALTLDVTEIGTPDIGTIYDSVEVVETTFADDAALQCVIQSMYAWVG
                     EAPAENFFEKRYTMSFTLGTPTEELKLQRSFEAIIGAHIGEVRYCEKRGNADAPDVVG
                     HLRLAFDLEADGRGYAKARAVTTQDTDLPGEVEDCIVVASRRWVFPATHSEKMFTYDF
                     VLPVPGRGPR"
     gene            complement(1575..>4314)
                     /locus_tag="VGB85_01500"
     CDS             complement(1575..>4314)
                     /locus_tag="VGB85_01500"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY0132713.1"
                     /translation="AEADGTSAQPLDQRRRERAAISGTVRDTEGATIAGALVCAAGDS
                     SLLSAMDTRQPRCAETGTDGRYRIDDLFGVRQRVSAGGAGFLPADHVHIKQGLRRRAI
                     DLRPGAEATDIDLTLERGGVEIRGIVEDLHGKPIAGAWLSSGGSASGTGIVWTRSGAD
                     GAYAMWVRPGTTTVTAYATGHAAGSATGPSEGHAFTVYLAPESVLRGKVFRAHDREPI
                     EGARVRASAHGVAVSTDAAGHFHFDGLPPGVYSPRVETDDGFGMAAEQVSLGLGETSW
                     PLMIAVQPAVFVEGRIAYENGEPCDNGALTLRESASGREIHDTTEPSGLVHLRGLLPG
                     TYTATVTCKGAIPAQRYPLMVVRDKDVVDQVWQIASGRSISGVLVDASDMPVVGATIV
                     ARPDDGSLSPPSVVSDDAGRFLLRGLAAASYQVVPLAHPRRTMPDEPVAVDLREADVT
                     DLRVPLTPTGEVRGTLRDAGDRPIAGAELALRTARGTQVAIAADDGRFWFASAAAGDS
                     AFDVLLGGAPLKVLAHARVRVTADTTTTAMLVTTAPTGKITGVLSDTKGAPVADALVE
                     VRPEGIDTMIDRPMLERVGGERPRRTDAAGHFELAGLPPAKYTVVAYRLGGGEARREH
                     VTPGETLALALTPTGRVTGTVALHRGRTPEAFVVDLLDLRTGDRRSGDFVGTDGAWGF
                     GNLPPGDYELRVRAREGEKKTTLTLAPGEERSRLRLELVGATMLRGTVLDLSGEPVPG
                     LEVGTGSVRELAADVATITDAEGRFELPRVAVGPVFVTVGPPPGRPSPFGAARVPIEV
                     QPDQKVVELAPIRVARRRIALGAARGDLGFTIVAGKADADPLLADLKVASVRPGGPAA
                     TAGLLPRDEIVTVDGQDVRGANRSLYATMSEVPPGTTLRLGLLRGAEVEVVASARR"
BASE COUNT          616 a         1571 c         1535 g          592 t
ORIGIN      
        1 gtcgggccaa cttgtcgagg ccatcctcgt gcgacgagac gatactgcta ccgatcatgg
       61 cggcacgcta tcacgcttca cgcgcgacat cccggcactc cgcgcaaacg ccgcagaaca
      121 ttgacctgca acgctttgcc ccgcggcggc atgctggggg tgaccaaaat gcccgcccac
      181 ggatcgctca cgaccgcgct cgacgacctg cgatggctgc ggcgcctcgc cttcaccctc
      241 gcccgtgacg ccgacgaggc cgacgacctc gtgcaggaca cgctggtgac cgcgtggcgc
      301 gagcccccgc gcgatctgac aggaccggcg cggccgtggc tctcgaccgt cctgaggaat
      361 ctctggcgaa tgcggcggcg ctccggtgca agacgcgagc gacgcgagca ggacgccgcc
      421 gtctccggaa ccccgacgca gcccgagcac gagctcgtgc gcctcgaggt gctgcgaacc
      481 ctggtggcag ccctcgaccg gctcgacccc ggggaccgca agatcatcgt gcggagatac
      541 ttcgagggtg agagcgccgc cgagatcgcg cgcgcgctcg cgatcccggc cgccaccgtg
      601 cgctcccgga tccaccgatc attaaaagtc ttacgagcgg cgctcgacga gaagttcgac
      661 gcgcgctcga cctggtcggc ggcgatcctc gccggcccac ccatatttcc gacgagccct
      721 ccgaaaggcg atgacatgag tatctttata aaaacagtgt tgggggcggg catcggcgcg
      781 gccgggtgcg tgggctggat cgggatgtcc gcacagcccg aatcgacgat tgatcccatc
      841 gagtccgtcg aacccgtgac gaccgacccg aagatggcgt gggagcagcg tcgtcagggc
      901 attcgtcgag tcctgccgcc ccttgcgatc ccgaccgcgc gcgacgcggg cgacagggag
      961 cagcgcgagc acgacgactt ccgcgagctg gtgcgcgcct gtctggagga cctcgagagc
     1021 gacgccagcg gcgccttgac cctcgatgtg acggagatcg gcaccccgga catcggcacg
     1081 atctacgact cggtcgaggt ggtcgagacg accttcgcgg atgacgcggc gttgcagtgc
     1141 gtgatccagt cgatgtacgc ctgggtcggc gaggcgcccg cggagaattt ttttgagaag
     1201 cgctacacga tgagcttcac gctcggtacc ccgacggagg agctcaagct ccagcgcagc
     1261 ttcgaggcga tcatcggcgc gcacatcggc gaggtccgct actgcgagaa gcgagggaac
     1321 gccgacgcgc cggacgtcgt cggacacctg cgtctggcct tcgacctcga ggccgacggg
     1381 cgcggttacg ccaaggcccg cgccgtgacc acgcaggaca ccgacctgcc gggcgaggtg
     1441 gaggattgca tcgtcgttgc gagccgccgg tgggtgttcc cggcgacgca cagcgaaaaa
     1501 atgttcacat acgacttcgt gctgccggtg cccggccgcg ggccgcgatg atcgatgatc
     1561 tatgatcgat ggaatcagcg tcgcgcgctc gccacgacct cgacctcggc gccgcgcagg
     1621 aggccgaggc gcagcgtggt gccggggggc acctcgctca tcgtcgcgta tagcgaacga
     1681 ttggcgccgc ggacgtcctg accatcgacg gtgacgatct cgtcgcgcgg gagcaacccg
     1741 gccgtggccg cggggccgcc cgggcgcacg ctcgcgactt tgaggtcggc gagcagcggg
     1801 tcggcgtcgg ccttgccggc gacgatcgtg aagccgaggt cgccgcgcgc ggcaccgagg
     1861 gcgatcctgc ggcgggcgac gcggatcggt gccagctcga cgaccttttg atcgggctgg
     1921 acctcgatgg gcacccgcgc cgcgccgaac gggctcggtc gacccggcgg cgggccgacc
     1981 gtcacgaaca ccggcccgac cgcgacgcgc ggcagctcga agcggccctc ggcgtccgtg
     2041 atcgtcgcga cgtcggccgc gagctcgcgg accgagccgg tgccgacctc gaggcccggc
     2101 accggctcgc cggagaggtc gagcaccgtc ccgcgcagca tcgtcgcgcc gacgagctcg
     2161 agccgcagcc gcgagcgctc ctcgccgggc gcgagcgtca gcgtcgtctt cttctcgccc
     2221 tcgcgcgcgc ggacccgcag ctcgtagtcg ccgggcggca ggtttccaaa gccccacgcg
     2281 ccgtccgtgc cgacgaagtc gccgctacgc cggtcgccgg tgcgcaggtc gagcaggtcg
     2341 acgacgaagg cttcgggcgt gcggccgcga tgcagcgcca ccgtgccggt gacgcgcccg
     2401 gtgggggtca gcgcgagcgc gagggtctcg ccgggcgtca cgtgctcgcg gcgcgcctcg
     2461 ccgccgccga ggcgataggc gacgaccgta tatttcgcgg gcggcaggcc agcgagctcg
     2521 aagtgtccgg ccgcgtcggt gcggcgcggt cgctcgccgc cgacgcgctc gagcatcggc
     2581 cggtcgatca tcgtatcgat gccctcgggc cggacctcga cgagagcgtc ggcgaccggc
     2641 gcccccttcg tatcgctgag cacaccggtg atcttgccgg tcggcgcggt ggtgacgagc
     2701 atcgccgtcg tcgtcgtgtc ggccgtgacg cgtacgcgcg cgtgggcgag caccttcaac
     2761 ggcgcaccgc cgagcagcac gtcgaacgcg gagtcacccg cggccgcgct ggcgaaccag
     2821 aacctgccgt cgtcggccgc gatcgcgacc tgcgtgcctc gtgcggtgcg cagcgccagc
     2881 tcggcgccgg cgatcggccg gtccccggcg tcgcgcagcg tgccgcggac ctcgccggtc
     2941 ggcgtcagcg ggacccgcag gtcggtgaca tcggcctcgc gcaggtcgac cgccaccggc
     3001 tcgtccggca tcgtgcggcg cgggtgtgcg agcggcacca cctggtagct cgcggctgcc
     3061 aggccacgca gcagaaagcg gccggcgtcg tcgctgacga ccgacggcgg gctcaggctg
     3121 ccgtcatccg ggcgcgcgac gatcgtcgcg ccgacgaccg gcatgtcgct ggcatcgacg
     3181 agcacgccgg agatcgaccg cccgctggcg atctgccaca cctggtcgac gacatccttg
     3241 tcgcgcacga ccatcaatgg atagcgctgc gccgggatcg cgcccttgca ggtgacggtc
     3301 gccgtgtacg tgcccggcaa cagaccgcga agatgcacga gcccgctcgg ctcggtggtg
     3361 tcgtggatct cgcggccgct cgccgactcg cgcagggtca gcgccccgtt gtcacacggc
     3421 tcgccgttct cgtaggcgat ccgcccctcg acgaacacgg ccggctgcac cgcgatcatc
     3481 agcggccacg aggtctcgcc gagcccgagc gacacctgct cggccgccat gccgaagccg
     3541 tcgtcggtct cgacgcgcgg gctgtacacg cccggcggca ggccgtcgaa gtggaagtgc
     3601 ccggccgcgt cggtcgacac cgcgaccccg tgcgcgctgg cccgcacccg cgcgccttcg
     3661 atcggctcgc ggtcgtgggc gcggaacacc ttgccgcgca gcaccgactc gggggccagg
     3721 tacacggtga aggcgtggcc ctcgctcggc ccggtcgcgc tgcccgccgc gtgtccggtc
     3781 gcatatgcag tgacggtcgt cgtgccgggt cgcacccaca tcgcgtaggc cccgtccgcg
     3841 ccggagcgcg tccacacgat cccggtgccg ctcgccgagc cgccgctcga cagccacgcg
     3901 cctgcgatcg gcttgccgtg caggtcctcg acgatgccgc ggatctcgac gccgccgcgc
     3961 tcgagcgtca ggtcgatgtc ggtcgcctcc gccccgggcc gcaggtcgat cgcacgccgg
     4021 cgcaggccct gcttgatgtg cacgtgatcg gccgggagga agccggcccc gccggcgctg
     4081 acgcgctggc ggacgccgaa caggtcgtcg atgcgatagc ggccatcggt cccggtctcg
     4141 gcgcagcgcg gctggcgagt gtccatcgcg ctcagcagcg acgagtctcc cgccgcgcac
     4201 accagcgcgc cggcgatcgt cgcgccctcg gtgtcacgca cggtgcccga gatcgcggcg
     4261 cgctcgcggc ggcgctggtc cagcggctgc gcggacgtcc cgtcggcctc ggcc
//
LOCUS       DASWNJ010000082         1756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_460975, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000082 DASWNJ010000000
VERSION     DASWNJ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460975"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1756)
                     /gene="aceE"
                     /locus_tag="VGB85_01505"
     CDS             complement(<1..>1756)
                     /gene="aceE"
                     /locus_tag="VGB85_01505"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEY0132714.1"
                     /translation="KLVWGSGWDRLLAKDKDGILLKRMEEVVDGEYQDFKSKNGAYVR
                     EHFFGKYPELKAMVADMSDDEVWALTRGGHDPLKVYAAYHEAVSTKGRPTVILAKTVK
                     GYGMGEAGEGQNITHQQKKMGEAHLKEFRDRFGLTLDDEQIKAIPFLRFEEGSPEQKY
                     LRERREALGGPLPARRRTSTALEVPSLQGFAAQLVATGEGREISTTMAFVRILNTLLR
                     DKKIGRHVVPIVPDESRTFGMEGMFRQFGIFSQVGQLYRPQDADQLMFYKEDKGGQIL
                     QEGINEPGAMSSWIAAATSYSTSNVPMIPFYIYYSMFGFQRVGDLAWAAGDMRARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHVFSATIPNCVSYDPTFAYEVATIVQDGLRRMYAEQ
                     EDVFYYITTLNENYEHPAMPEGAEEGIRKGMYLFRTVEGGGKRGPSVQLLGSGSILRE
                     VIAAADMLRDDWGVGSRVWSCPSFTELAREAADAERWNLLHPEGEPRKSWVETCLGGG
                     EAGPVVASTDYIRLFADQIRPYVPGRYRVLGTDGFGRSDYRRRLRHFFEVDRRWVVLA
                     ALESLAKEGKIPASRVAEA"
BASE COUNT          243 a          609 c          606 g          298 t
ORIGIN      
        1 tcgcctcggc gacgcgcgag gccgggatct tgccctcctt ggccaggctc tcgagcgcgg
       61 ccagcaccac ccagcgccgg tcgacctcga agaagtgccg caggcggcgc cggtagtccg
      121 accggccgaa gccgtcggtg ccgagcacgc ggtagcggcc ggggacgtat ggccggatct
      181 ggtcggcgaa caggcggatg tagtcggtcg aggcgacgac cggcccggcc tcgcccccgc
      241 cgagacaggt ctcgacccag ctcttgcggg gctcgccctc cgggtgcagc aggttccacc
      301 gctccgcgtc ggcggcctcg cgggcgagct cggtgaagct cgggcagctc cagacgcgcg
      361 agccgacgcc ccagtcgtcg cgcagcatgt cggccgcggc gatcacctcg cgcaggatcg
      421 agccggagcc caggagctgg acgctgggcc ccctcttgcc gccgccctcg acggtcctga
      481 agaggtacat ccccttgcgg atgccctcct cggcaccctc gggcatggcc gggtgctcgt
      541 agttctcgtt gagcgtcgtg atgtaataga acacgtcctc ctgctcggcg tacatgcgcc
      601 tgagcccgtc ctggacgatg gtcgcgacct cgtaggcgaa ggtcgggtcg taggagacgc
      661 agttcgggat cgtggccgag aagacgtggc tgtgcccgtc ctcgtgctgg aggccctcgc
      721 cgttcagcgt ggtgcggccc gaggtgccgc cgatcaggaa gccccgggcg cgcatgtcgc
      781 cggcggccca ggccaggtcg ccgacgcgct ggaaaccgaa catcgagtag tagatgtaga
      841 aggggatcat cggcacgttc gacgtgctgt aggaggtcgc cgccgcgatc caggacgaca
      901 tggcgccggg ctcgttgatc ccctcctgga ggatctgccc gcccttgtcc tccttgtaga
      961 acatgagctg gtcggcgtcc tgcggccggt agagctggcc cacctggctg aagatgccga
     1021 actggcggaa catgccctcc atgccgaagg tgcgcgactc gtccggcacg atcggcacca
     1081 cgtggcggcc gatcttcttg tcgcgcagca gggtgttgag gatccgcacg aaggccatgg
     1141 tcgtgctgat ctcgcgcccc tcgccggtgg ccacgagctg ggcggcgaag ccctggagcg
     1201 agggcacctc cagcgccgtc gaggtgcgcc gccgcgccgg gagcgggccg ccgagcgcct
     1261 cgcggcgctc cctgaggtac ttctgctccg ggctgccctc ctcgaagcgg aggaacggga
     1321 tcgccttgat ctgctcgtcg tcgagcgtca ggccgaagcg gtcgcggaac tccttgaggt
     1381 gcgcctcgcc catcttcttc tgctgatggg tgatgttctg cccctcgccg gcctcgccca
     1441 tgccgtagcc cttgaccgtc ttggccagga tcacggtcgg cctgcccttg gtgctcaccg
     1501 cctcgtggta ggcggcgtac accttgagcg ggtcgtgccc gccgcgggtg agcgcccaga
     1561 cctcgtcgtc cgacatgtcg gcgaccatgg ccttcagctc ggggtacttg ccgaagaagt
     1621 gctcgcggac gtaagcgccg ttcttcgact tgaagtcctg gtactcgccg tcgacgacct
     1681 cctccatgcg cttgagcagg atgccgtcct tgtccttggc cagcagcctg tcccagccgg
     1741 agccccagac gagctt
//
LOCUS       DASWNJ010000083         2545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_495617, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000083 DASWNJ010000000
VERSION     DASWNJ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495617"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(570..1220)
                     /locus_tag="VGB85_01510"
     CDS             complement(570..1220)
                     /locus_tag="VGB85_01510"
                     /inference="COORDINATES: protein motif:HMM:NF027592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HEY0132715.1"
                     /translation="MRTICLLSLLLAVGCDNAAKPSEPAPVAAAPAASAAPSASAASA
                     APAETKVAAADPAKADEEGCIYKDTQKDEEAGCPHGGAEHSAPASATPGHFGAVFAIK
                     ETRPLSQVLAAGKEFKDPVQVSGQVDSVCQKKGCWLVVKDGEAQARILMKDHSFTVPM
                     DSKGKPVVVEGTLEARTFNEAQVKHLEKDAGKDPAAVTGERTEYVLTATAVELKNS"
     gene            1358..>2545
                     /locus_tag="VGB85_01515"
     CDS             1358..>2545
                     /locus_tag="VGB85_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY0132716.1"
                     /translation="MLPRRTLRLALAAGLLGCVPATAPAPAAPTTKPVAARPRLAIVL
                     VVDQMRADYLERYADLYRGGLARFRREGAWFTQAHLRHALSYTAPGHASVSTGSDPAR
                     HGIIANDWFDRASGHEMYAAEDPSAPLMFTTSTTVTLPGRSPRQLERPALGDWLKASE
                     PAARVFSVALKDRAAVLMGGHKPDRAYWYSAEVAGYVSSSWYAGGAPPPSWVAEFNAS
                     GAVFDRFARGWIRSRDISAYLRSGPDHVDAEADGEQTRFPHLFDDGTPEARAAYAKKL
                     LTTPFGDELTLRFARRLIEAEQLGADDTPDLLMISCSSADYIGHAYGPFSHEAEDYYL
                     RLDDWLGEFLAYLDEHVGKDSYVLALTADHGALPLPEEARLRGHGSARRVPGPAYRAR
                     ANEA"
BASE COUNT          354 a          930 c          861 g          400 t
ORIGIN      
        1 gaagcggttc tgttgatcgt tgcctccgta gttgctgttg cggtcgtcac ggtcgttgtt
       61 gcggtcgttc ggtctcatgc tgcctccaag cgcgaagttt tgtgagatct gctccgtcag
      121 ggggagccaa tctcggtgga cagggtatgg ggcaagttcc agcgggcacc cgtcgcgcag
      181 gaggcagtgc acatataacg ggtggagatt gatttctgtg cacattgccg catcccgggc
      241 agcggcttgc gcagatgacg cgggccataa tctgcctccg gggctaccag gcctcataaa
      301 cgtgggccta tcaccttcct gtcatgtcga tcaaagcccg tccctcagcg ggcgcggcgc
      361 cgctggtatt aacccgcgct atgcgagcaa gcggcgaaac aatgtacata taacgatgat
      421 taccacgcgc agatcgtcca tcgcttttgc gtccgcggat ccgagcattc gccgcaccgg
      481 ctgcgcggtg gcacggtgca gcccgccgga cgcacctcgc gacgacgccc ggccgctggc
      541 gagcaggccg ggcgtcggac gacgggggct cagctgttct tgagctcgac ggcggtggcg
      601 gtcagcacgt actcggtgcg ctcgccggtg acggcggcgg ggtccttgcc ggcgtccttc
      661 tcgaggtgct tgacctgggc ctcgttgaag gtgcgcgcct cgagcgtgcc ctcgacgacg
      721 accggcttgc ccttgctgtc catcggcacg gtgaagctgt ggtccttcat caggatgcgg
      781 gcctgggcct cgccgtcctt gacgaccagc cagcagccct tcttctggca cacggagtcg
      841 acctgaccgc tgacctgcac ggggtccttg aactccttgc cggcggcgag cacctgcgac
      901 agcggcctgg tctccttgat cgcaaacacg gcgccgaagt gaccgggggt ggcgctcgcc
      961 ggagcgctgt gctcggcacc gccgtgcggg cagccggcct cctcgtcctt ttgcgtgtcc
     1021 ttgtagatgc agccctcctc gtcggccttg gccgggtcgg cggcggcgac cttggtctcg
     1081 gcgggcgcgg ccgatgcggc cgatgcggaa ggagcggccg atgcggcagg ggcagcggcg
     1141 accggggccg gctcgctggg cttggcagcg ttgtcacaac cgacggcaag gagcagggac
     1201 agcaggcaga tcgttcgcat cggcaaatcc tcgtccggag tgtgagcggc cggtccccgg
     1261 cctgtcaacg cgcgcccgaa cgcgcagacg ggccctggct cacacctggg ccgcccctgg
     1321 ccttgtgcgg gcccgcgacg tacaacagac ccccgccatg ctcccgcgcc gcacgcttcg
     1381 cctcgcgctc gccgccggcc tcctcggctg cgtgccggcg accgccccgg cgccggccgc
     1441 cccgaccacg aagcccgtgg ccgcccgccc ccgcctggcg atcgtgctcg tcgtcgacca
     1501 gatgcgggcc gactacctcg agcgctacgc cgacctgtac cgcggcggcc tggcccgctt
     1561 tcgccgcgag ggcgcatggt tcacgcaggc ccacctgcgc cacgcgctca gctacacggc
     1621 ccccggccac gcgtcggtgt cgaccggctc cgaccccgcg cgccacggca tcatcgccaa
     1681 cgactggttc gaccgcgcgt cgggccacga gatgtacgcc gccgaggacc cgtcggcgcc
     1741 gctgatgttc acgacgagca cgaccgtgac cctgcccgga cgctcgccgc ggcagctcga
     1801 gcggccggcg ctcggcgact ggctcaaggc cagcgagccc gccgcgcgcg tgttctcggt
     1861 cgcgctcaag gaccgcgccg ccgtgttgat gggcggccac aagcccgacc gcgcctactg
     1921 gtacagcgcc gaggtggccg gctacgtgtc gtcgagctgg tacgccggcg gcgcgccgcc
     1981 gccgtcgtgg gtcgccgagt tcaacgccag cggcgcggtg ttcgaccgct tcgcccgggg
     2041 atggatccgc tcgcgcgaca tctcggcgta cctgcgctcc ggccccgacc acgtcgacgc
     2101 cgaggccgac ggcgagcaga cccgcttccc gcatctgttc gacgacggca cccccgaggc
     2161 ccgcgccgcg tacgccaaaa agctgctgac gacccccttc ggcgacgagc tgacgctgcg
     2221 cttcgcgcga agattgatcg aggcggagca gctcggcgcc gacgatacgc ccgacctgct
     2281 gatgatcagc tgctcgagcg cggactacat cggccacgcc tacgggccgt ttagccacga
     2341 ggccgaggac tactacctgc gcctcgacga ctggctcggc gaattcctcg cctatctcga
     2401 cgaacatgtc ggaaaagaca gctacgtgct cgccctgacc gccgaccacg gcgccctgcc
     2461 gctgcccgag gaggcccgcc tgcgcggcca tggctccgcc cgccgcgtgc ccggcccggc
     2521 gtaccgcgcg agggccaacg aggcg
//
LOCUS       DASWNJ010000084        17022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_107633, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000084 DASWNJ010000000
VERSION     DASWNJ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 17022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17022
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107633"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="VGB85_01520"
     CDS             <1..758
                     /locus_tag="VGB85_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132717.1"
                     /translation="FFFAAYAAVPADRENNHHRESILGSIRGSLLLENERSGAAAPLC
                     RLRETLQAHVASVRTIFADDLGRVEVVENERRVGLVETQLAAYPANVCDPEPPPPEPV
                     VTPAPVAPPVPQSAAPPPTASASPSDAPSPHRLRIAGGVTLGLGAVLLGAMTVGVVQH
                     ARITARLDARAAGLAPGRPLDPVIWDDVQHDLDRARLHRGLAIGAGVSAALALGGGVA
                     LFIVARGRTRDRLALAPWWLSSGTGLIVRVRLP"
     gene            807..1928
                     /locus_tag="VGB85_01525"
     CDS             807..1928
                     /locus_tag="VGB85_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132718.1"
                     /translation="MTHPTHHFLRFLGSCLLLLLMGCSEAPSRGGPDLGSTLTTGDPG
                     GPSSGPGGSGDLPTTGEAVESGTASTTTGTTGTTGTVTSTTGEPGSICGDDIVEGDEQ
                     CDDGEANSDHAYCTSQCALNFCGDGHVFVGWELCDEGKANSDFYGSTCGTQCVPTARC
                     GDHIVQADEGEECDLGPDNGGPLGDAQGILCESSCRSKSLRAFVTSQAFSGNLGGLYG
                     ADKKCRDAAAAAGLGEAYRFYAYLSTPDFSANDRFPGPGAEPLPYRLVTGKKIADSHA
                     KLLYQGPLDEGVSVTELGTSLYDELVVTNTAPNGNSYSPDQHCLAWTSDDPLLKARIG
                     LTFPSDPDDIPAWVQDGEWSSWATYKCDKPVYHLYCLEI"
     gene            1982..2902
                     /locus_tag="VGB85_01530"
     CDS             1982..2902
                     /locus_tag="VGB85_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0132719.1"
                     /translation="MTHLTPSLTPRLVFVCLPSLLALQTACAIERLEAIFDEHGQTYV
                     TTDVADSETTAADTTGTDTTAVGPDDSSSGAGSSTGAGGGQDASGEPDTSTGSTTDET
                     TGGVAVCGNGVVETFAVPPEECDDGNQIADDGCDETCAADRVMFVSSSLYKAGDFKSL
                     HLADPLCAQAANDAGLANWLKFRAWLSDSTTHARDRFKFSRGRIVLVNGLVVADSWID
                     LLAGKLQNPIEVTEKSETYHGGVWTGTDPQGVGVVGSTHCDDWTSFSGKKVAYYGYSD
                     RATPEWTLSALDDNPISCLFDFAVYCLEER"
     gene            2922..3812
                     /locus_tag="VGB85_01535"
     CDS             2922..3812
                     /locus_tag="VGB85_01535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0132720.1"
                     /translation="MTYPAPHRDPSYCASLLALLLGCGESPPHDSPGTNTADTNAASM
                     VDTSTAGAAASSDGGETTAAAASIDAGEAGQDASGEPDTSMGSTTEETTGGGAVCGNG
                     VVEAFAVPPEECDDGNQIADDGCDDTCAADRVMFVSSSLYQAGDFKSLHLADPLCAQA
                     ANDAGLANWLKFRAWLSDSTTHARDRFKFSRGRIVLVNGLVVADSWIDLLAGKLQNPI
                     GVTEKSETYHGGVWTGTDPQGVGVVGATHCEDWTSFSAYKTAYYGYSDRATPEWTLSA
                     LDDNPIPCAFDFAVYCLEER"
     gene            complement(3828..4697)
                     /locus_tag="VGB85_01540"
     CDS             complement(3828..4697)
                     /locus_tag="VGB85_01540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0132721.1"
                     /translation="MTTNMQGKTVLITGGNTGIGKATAIALARMGANVTITSRNPDKG
                     EAALADIRQQAGSDRVECMRLDLASLASVRRFAAEFLATHPRLDVLINNAGLVLSTRS
                     ETEDGFESTFGVNHLGHFLLTNLLLPRLKESAPARIVVLSSGAHMGAIGGLDFDDLHA
                     RRSYRGMPVYCRSKLANLLFTVELADRLRGTGVTVNAVHPGLVGTEFGGADDTTGVFR
                     WIVKMTHWAYISPEKGARTSVHVASAPELEGVSGKYFVRSKPARSSRAAQDRGAALRL
                     WEISEQLTGASPI"
     gene            4752..5534
                     /locus_tag="VGB85_01545"
     CDS             4752..5534
                     /locus_tag="VGB85_01545"
                     /inference="COORDINATES: protein motif:HMM:NF019537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease A inhibitor family protein"
                     /protein_id="HEY0132722.1"
                     /translation="MRTTSLRLPALVLACVLPACGSDPDTTTSSETSGTATTGSATAT
                     TGENTDPTTGATGTGTGDTSTSEPGTTGTSEPGTTDVVTTGDTSTGGPDTTVTSDTGD
                     TSTGGGDGLGQAAQDAAAALELAVDGVLYLSESEDTWTVFALAGAAPVTDKNVKDVIA
                     GIYVPHDDVPLADRVVELRTLAQLMDPLTVMQDWWGDFEKMQAEDYKQIRAIFEGQLS
                     NPQVFRLGEQSGNDLVGQVDVFVIGETVDGDLVGMFAIAVET"
     gene            5534..6859
                     /locus_tag="VGB85_01550"
     CDS             5534..6859
                     /locus_tag="VGB85_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132723.1"
                     /translation="MRAPLLTLCMLLACNAGGGDTDSDTGDTGEAGLAPVRVFAVGNH
                     HGLDYVDSYAAFEAEMQRLMAVVQPNLSKDHPNLVVFGEDVGLPAAFLGARGASARAS
                     TTAQAGYLAVAIAYEDAVVHYTQVYPHITFNRAISLALTDTMARAFFGTFPKLAKQHG
                     VWLSACTLLADTTESTDPADIAAFGDPELTGQSSVYLPVDEHVYNVCYIWDDAGEVVG
                     ASRKVNLVALEGMDLLDLSPEILENVEAFDTPFGRVAIAISLDAFVPAYVQHINDLGA
                     QIVLQNDANPGAWAIIQPQGGHDGPPDGSMVWQPEEWEDSTLRMVTDAAYPNIEFNVC
                     PMIVGNLFDIPFDGQSSITARHPPADNPARGYIGNAPSDRFLALAPWAFPDPGDADPG
                     LTLEQRREAMLLLGEQLAPGSGHASENKYREDVIWADIQVIQVSAPPAP"
     gene            complement(6861..7934)
                     /locus_tag="VGB85_01555"
     CDS             complement(6861..7934)
                     /locus_tag="VGB85_01555"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEY0132724.1"
                     /translation="MLETPAYAAPDPRSPLAPHTISRREPGPHDLLIDILYCGVCHSD
                     VHQARDEWGNGIFPMVPGHEIVGRVAQVGIEVHNNFREGDIVGVGCFVDSCRECEACT
                     AGEEQYCGKGCTNTYNGRERASGEPTHGGYSTRITVHQDYVLRVPEGIPLERAAPLLC
                     AGITMYSPLKHFGVKAGDKIAIVGLGGLGHMGVKIAAAMGAHVTVLSHSESKREDAMR
                     LGASDFIVTKDPGVFTEHAGRFDFILDSVSAQHDYNAYLGLLRLDGTMVLVGAPEPTP
                     LSAFPLIVGRRRLAGSMIGGIRETQEMLNFCAAHNVASDVEVIDIKDINAAYDRMVRS
                     DVRYRFVIDIASLKASEATAAAT"
     gene            8132..8629
                     /locus_tag="VGB85_01560"
     CDS             8132..8629
                     /locus_tag="VGB85_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132725.1"
                     /translation="MQTIIAQFEQVIHAESAVRALLERKYDRADISFIANDVKSDTAL
                     GDGSGTPAGAGQASIGLLLGIGSFMIPGVGPLLAAGPLAVGVAGAASGVAQKDAHWLD
                     PLLGGYGIPEASARAYSERLHRGGALILIPAREEVSDNIIGVLESSGAVQVDTHARDP
                     KSSGA"
     gene            complement(8635..9195)
                     /locus_tag="VGB85_01565"
     CDS             complement(8635..9195)
                     /locus_tag="VGB85_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132726.1"
                     /translation="MASTPHSVEFAENDRFVMVAGPGWTLQIDATGGHPTLIPRGQLR
                     VVDDLLLLFTEGEHRRSGAGYLPQVHRLTGDGRVLWSLRARCVGDPVRTAEGLLLPVR
                     LTRSRLGPPVLHVQLRDVDTGALLAVWPVRPPPVLEPAYAHALAGVGARLVTREGAVQ
                     VAVSAWFTGPHAPPRGAGGFDVLIAL"
     gene            complement(9234..9947)
                     /gene="pepE"
                     /locus_tag="VGB85_01570"
     CDS             complement(9234..9947)
                     /gene="pepE"
                     /locus_tag="VGB85_01570"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF003642.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HEY0132727.1"
                     /translation="MSRLLLLSSSNVHGHGYLDHAEAELLDFLGPVKRLLFVPFALHD
                     RDAYTAKVASRLAPLGIAVEGLHTLDDPDDARAAVAAAQAVFVGGGNTFRLLSEVYRL
                     DIRDTLFERVDAGMPYIGSSAGTNLAGLTIGSSNDMPIVYPPSFVALGLVPFNFNPHY
                     LDPDPHSTHKGETRETRIHEFHEHNHQPVLGLREPAMLRREGDRLVLRGEAGARLFRA
                     GQEPVEQAPGADLSALLHA"
     gene            10039..10434
                     /locus_tag="VGB85_01575"
     CDS             10039..10434
                     /locus_tag="VGB85_01575"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEY0132728.1"
                     /translation="MADVWPYEVEPFQCHRCGNCCRGDGYVVMTPDDVGRAAAHLGRS
                     EAEFLAEFCATQPDGSVHLKDQSDALRSCVFLTDYNLCRVHAAKPTQCEGFPMKWRPS
                     NVLEICAGMRAAAGLPPPQRRGVGLRARG"
     gene            complement(10504..11976)
                     /locus_tag="VGB85_01580"
     CDS             complement(10504..11976)
                     /locus_tag="VGB85_01580"
                     /inference="COORDINATES: protein motif:HMM:NF025224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEY0132729.1"
                     /translation="MTVTGSTSGADDSTSVSAGQTSAPEPTSEASGAPDSTSASEHTS
                     TTTADTSTGEPVDCAEIVIPDPGLFMHLLDELDLEAGPIPPALALTTTTLSFVGEPEV
                     DSLAGLECFANLENLLIIGPGGITDLGPLSGLGELESVQLDLQDVGDLGPLVGLPLRH
                     LSLRGSNIVDLAPIAALPDLRHLDVDKNPVVSFAPLADHPALTRLSAAGTTPIDLVGL
                     GEIPHLAKLTLAGAGVTDLGPLAGAVGLTELDLSFNDIIELGPLAGMTQLYQLELDRN
                     QIATVDVLTAMPELVTLRLRHNAIADVTPVASLPALRTLDLAENAITTIAPLAGLLEL
                     RSLHLEVNQISDVAPLAALAALTELDVSDNPLASGISELTALTELTDLGLEHTGISDL
                     ATISPVTLRTLRAGGNAITDLAPLAGHTKLRVLKLADNAITTIDPILGAPFWADDCVE
                     VDFRGNPLDAVSLTTSLPGLCDDNQWVLWDEGECYYCMLR"
     gene            complement(12152..12796)
                     /locus_tag="VGB85_01585"
     CDS             complement(12152..12796)
                     /locus_tag="VGB85_01585"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEY0132730.1"
                     /translation="MPQQSHLQQPRATTCNQWFTLFTRITASMFNVAPTKVATIMTAG
                     AGMHAGIDAALHDKTAGMTTRASISWPLLVVAAGALTLAACKKSSDTTAPDSGSDVSQ
                     PEDNGGDAAPEEDSAPEFLTVDVFEDVVNQKTSDVTDCFAAAKEKKPDLGGKLMLEFT
                     IDGTGKVSEVKTESGSTVNDPGLTTCVTDKARTWAFPKTRDGQPMTLPYSFNLT"
     gene            12885..16154
                     /locus_tag="VGB85_01590"
     CDS             12885..16154
                     /locus_tag="VGB85_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEY0132731.1"
                     /translation="MGLELSLDDIRRAWDARDPDLANLIVTLVAAPEPKPTTPVREGA
                     LSFASFTAQLATGKFRKSTPQERARYRIDTMRALEVANAEVPLPPRLQLHSVIVELWQ
                     KNGAYERAVLLELIRKVPLCWGPWRGLKRVFKEAEERNDTEVLGALAARLDAAYATKA
                     SSYREISRRTVGYLVRRAWRYLRRMGQTLAPAYVDAAVDFLRAYDDSVAFTRTWVYNH
                     ILSHETHKYTRRNFKLHRSDKPALTERAYAELWRRTPRPVLALLERASSEQVRKFAVS
                     ALKTDFRASLREVEAPWVVRLISVQSATVDEFVIWLLSNVPRFEQGGFRELGLHEPVL
                     RLLDSSNDAARAYAAAYARTHARDLELGRLLRLANNSHAEVRKLAADLLGDRDPRKDI
                     GLDAWGQLLGTQYGHDLAATALRKHFSARELTPAWFADRLLDERGKVFEFASDLLTKV
                     HTHKSLGAEFFRGLLDDPRVSAQAAKFALDALARFPGEDLGKEFLGRMILHPNTRPAL
                     LEWITEERIKAEDLGAEFWRAVAFRPSWDADPWIKELIASGRPWARELQFSEELARTA
                     LGFLSDVRRFSPGALGFDWLMQLALRPEPHYREFALEYMTKALVPADFAPKVEAPKQS
                     KAAAPKTIDLGGKSFLFTGKLATMVRDEAEAKVTGAGGKNSSSVTAKLDYLVIGDEGS
                     PLYGGGRKGSKQVAAEKLVEKGAGIKIISETAFLQMLAGEQREFTADSVTAGCDRLWE
                     MATGPGPADAPQASFARHYIRRHHPDISLALTDRPVDPGAEIPTSYLSWARVKPLLVD
                     ARAPVRQFGVELARWELARWAPPINEIVEVTEAPYPEVREFFAKALLADESVEHRRYR
                     VDPASLTASAVYGFCESLVSETRGLGMQLIHRYPKLAIPEELFRLSESPDRQVTAFVV
                     RTIWSLYHERGITEGWKPAPRAPKDAGKPGKKTEVKGPAVPVEGTGAPARPEKPPASG
                     EALQAFMRRILFSIPPTKLPVEAARAAEAQVEGAPQPDEPKAKRRARPLPARKAKLSL
                     IEAMRDLAIDDRGFAGLAAPLLREFMGSRGASERAACMVALARIGGAHPDLKVLTEDA
                     A"
     gene            16154..>17022
                     /locus_tag="VGB85_01595"
     CDS             16154..>17022
                     /locus_tag="VGB85_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEY0132732.1"
                     /translation="MSTATTSDKFTSDKYTQIRGELRALHAQGSDLWITTTHPQGAAT
                     ALYKLNLDSGDLQVSPLGGGAVALIADDSHLYLAGTDGHIYKGGLDGKAVKAIGPKLE
                     PAPTALALAVKDRLAVVCGKELVIVARKDGKEIQRLPLNELGSAIASDPSGVWLVVGG
                     DRGSLSVFDCEDKDRYFAAESKKLHEGAVTGLLFELEELRVMSVGNDNRLLTTHVRGQ
                     LEAEDRGGKNGHSDRTTALIPGIADKFYTAGLDSTIKTWTRGSGQRRPSTQKDGLAKV
                     HALALAELKGRPCL"
BASE COUNT         2603 a         5830 c         6104 g         2485 t
ORIGIN      
        1 aattcttctt tgccgcgtac gcagcggtcc cggccgaccg cgagaataat catcatcgag
       61 agagcatcct cggatcgatc cgcgggtcgc tcctgctgga gaacgagcgc agcggcgcag
      121 cggcgccgct gtgtcggctc cgcgagacgt tgcaggcgca cgtcgccagc gtccgcacga
      181 tcttcgcgga cgatctcggg cgcgtcgagg tcgtcgagaa cgagcggcgc gtcggcctcg
      241 tggagaccca gctggcggcc taccccgcga acgtctgtga ccccgagccg cccccgccgg
      301 agcccgtggt cacgccagcg cccgtggccc ctccggtccc gcagtccgcg gccccgccac
      361 cgacggcgag cgcctcgccc tcggacgcac cctcgcccca ccgccttcgc atcgccggcg
      421 gcgtgacgct cggccttggg gccgtcctgc tcggagcgat gaccgtcggc gtggttcagc
      481 acgcacgcat caccgctcgc ctggacgcgc gggcggccgg gctcgcgccg ggtcggccgc
      541 tcgacccggt gatctgggac gacgtgcagc acgacctcga ccgggcccgc ctccatcgcg
      601 gcctggccat cggggccggc gtctccgcgg cgctcgccct cggtggtggg gtcgcgctgt
      661 tcatcgtggc cagaggccgc acccgggacc gtctcgcgct cgcgccatgg tggctttcct
      721 cggggacagg tctcatcgtg cgcgtgcgtc tgccctgaag cttcgatcct caaccctgtc
      781 cgctcgactg ccacgaaggc cctgtcatga cccacccgac ccaccacttc ctccggttcc
      841 tcggctcctg tctcctcctg ttgctcatgg gctgcagcga ggcgccctcg cgcggcggcc
      901 ccgacctcgg gagcacgctg accaccggcg acccgggcgg ccccagcagc ggacccgggg
      961 gatccggtga cctccccacg accggcgagg ccgtcgagtc cggtacagcg agcacgacca
     1021 ccgggaccac gggcacgacc ggcaccgtca cgagcacgac cggcgagccc gggtcgatct
     1081 gcggggacga catcgtcgag ggcgacgagc agtgcgacga cggtgaggcc aacagcgacc
     1141 acgcctactg cacctcgcag tgcgcgctca acttctgcgg cgacggccac gtgttcgtcg
     1201 gctgggagct gtgcgacgag ggcaaggcca atagcgattt ttacggcagc acctgtggca
     1261 cccagtgtgt gccgacggcg cgctgtggtg accacatcgt tcaggcggac gagggcgagg
     1321 agtgcgacct cgggcccgac aacggcggcc ccctgggtga cgcacagggc atcttgtgcg
     1381 agagctcgtg ccgaagcaag tcgctgcgcg cgttcgtgac gagccaggcg ttctcaggaa
     1441 acttgggtgg cctgtacggc gccgacaaga agtgccgcga cgccgcggcg gccgcgggtc
     1501 tcggggaggc ctaccgcttt tacgcctacc tgagcacccc cgacttctcg gccaacgacc
     1561 gcttccccgg ccccggcgcc gagccgctcc cgtacaggct cgtcaccggc aaaaagatcg
     1621 ccgactcgca cgcgaagctg ctgtatcagg gcccgctcga cgagggggtg tcggtgaccg
     1681 agctcgggac cagcctgtac gacgagttgg tggtgaccaa cacggccccg aacggcaaca
     1741 gctacagccc ggaccagcac tgtctggcct ggaccagcga tgacccgctg ctcaaggctc
     1801 gcattggcct caccttcccg agcgaccccg acgacatccc ggcgtgggtg caagacggtg
     1861 aatggagcag ttgggcgacg tacaagtgcg acaaacccgt gtaccacctc tactgcctcg
     1921 agatctgacc cgcgcagacg ctgctttggt ccctgctcgc ctcgcgcccc aggaagctct
     1981 catgacccac ctgactccat ccctcacccc ccggctcgtg ttcgtatgtc tcccttcgct
     2041 gctggcgctg cagacagcct gcgccatcga gcgcctcgag gcgatcttcg acgagcacgg
     2101 gcagacctat gtgacgaccg acgtggcgga cagcgagacg acggccgcgg acacgaccgg
     2161 gacggacacg accgccgtgg gtccggacga cagcagttcg ggcgccgggt cgagcaccgg
     2221 agccgggggc ggtcaggacg ccagcggtga gcccgacacg agcacgggca gcacgacgga
     2281 cgagacgacc ggtggagtgg cggtgtgcgg caacggggtg gtggagacgt tcgcggtacc
     2341 gccggaggag tgtgacgacg gcaaccagat cgccgatgac ggttgcgacg aaacctgcgc
     2401 cgcggacaga gtgatgttcg tttcgagcag cctctacaag gccggtgact tcaagagcct
     2461 gcacctcgcg gaccctctgt gcgcgcaggc tgcgaacgac gcgggactgg cgaactggct
     2521 gaagtttcgc gcgtggctca gcgacagcac gacgcacgcg cgcgaccggt tcaagttcag
     2581 ccggggtcgg atcgtgctgg tgaacgggtt ggtggtcgcg gactcgtgga tcgacctgct
     2641 cgcgggcaag ctgcagaacc cgatcgaggt gaccgagaag tctgagacct accacggcgg
     2701 ggtgtggacc ggcacggacc cgcagggcgt aggtgtcgtc ggctccacgc actgcgacga
     2761 ctggacctcg ttctcgggca aaaaagtcgc ctactacggt tacagcgacc gcgccacgcc
     2821 cgagtggacg ctgtccgcgt tggacgacaa cccgatctca tgcttgttcg acttcgcggt
     2881 gtactgcctg gaagagcgct gatcaccccc gagggcttgt gatgacctac ccagcccccc
     2941 accgcgaccc gtcgtactgc gcctcgctcc tcgcgctgct cctcggatgc ggcgagtcgc
     3001 caccccacga tagccccggc acgaacacgg cggacacgaa cgccgcgagc atggtcgaca
     3061 cgagcacggc cggcgccgcg gcgagcagtg acggcggtga gacgaccgcc gccgcagcca
     3121 gtatcgacgc cggggaggcg ggtcaggacg ccagcggaga gcccgacacg agcatgggca
     3181 gcacgacgga ggagacgacg ggtggaggtg cggtgtgcgg caacggggtg gtggaggcgt
     3241 tcgccgtgcc gccggaggag tgcgacgacg gcaaccagat cgcggacgat ggttgcgacg
     3301 acacctgcgc cgcggacaga gtcatgttcg tctcgagcag cctctaccag gccggcgact
     3361 tcaagagcct gcacctcgcg gaccccctgt gcgcgcaggc tgcgaacgac gcggggctgg
     3421 cgaactggct gaagttccgc gcgtggctca gcgacagcac gacgcacgcg cgcgaccggt
     3481 tcaagttcag ccggggtcgg atcgtgctgg tcaacgggct ggtagtcgcg gactcgtgga
     3541 tcgacctgct cgcgggcaag ctgcagaacc cgatcggggt gaccgagaag tccgagacgt
     3601 accatggcgg ggtgtggact ggcaccgacc cgcagggtgt cggtgtcgtc ggcgccacgc
     3661 actgcgaaga ctggacctcg ttctcggcct ataagaccgc ctactacggc tacagcgacc
     3721 gcgccacgcc cgagtggacg ctgtccgcgc tggacgacaa cccgatccca tgcgcgttcg
     3781 acttcgccgt ctactgcctc gaggagcgct agcccctcac gcccgcctca gatcgggctc
     3841 gcgccggtca gctgctcgct gatctcccac aggcgcagcg cggcgccgcg gtcctgggcc
     3901 gcgcggctcg agcgggcggg cttgctgcgc acgaagtact tgcccgacac gccctcgagc
     3961 tcgggcgccg aggcgacgtg caccgaggta cgcgcgccct tctccgggct gatgtaggcc
     4021 cagtgcgtca ttttgacgat ccagcggaac acgccggtgg tgtcgtcggc gccgccgaac
     4081 tcggtgccga ccaggcccgg gtgcacggcg ttgacggtga ccccggtgcc gcgcaggcgg
     4141 tcggcgagct cgacggtgaa cagcaggttg gcgagcttcg agcggcagta caccggcatg
     4201 ccgcggtagc tgcggcgcgc gtgcaggtcg tcgaagtcga ggccaccgat ggcgcccatg
     4261 tgcgcgccgg acgagagcac gacgatgcgg gccggcgcgc tctccttgag ccgcggcagc
     4321 agcaggttgg tcagcaggaa gtggccgagg tggttgacgc cgaaggtcga ctcgaagccg
     4381 tcctcggtct cgctgcgggt ggagagcacg aggccggcgt tgttgatcag cacgtcgagg
     4441 cgcgggtgcg tggcgaggaa ctcggcggcg aagcggcgca cgctggcgag cgaggcgagg
     4501 tcgaggcgca tgcactcgac cctgtcgctg ccggcctgct ggcggatgtc ggcgagcgcg
     4561 gcctccccct tgtcggggtt gcgcgaggtg atcgtgacat tcgcgcccat gcgggccagc
     4621 gcgatcgccg tcgccttgcc gatgcccgtg ttgccgcccg tgatcagcac tgtcttgccc
     4681 tgcatgttcg tcgtcatcgc ggcgcgtcct agcagctttg ccccgcccgc gccaccgagc
     4741 taaaagagcg cgtgcgaact acttcactgc gtttacctgc cctcgtcctc gcttgcgtgc
     4801 tcccggcctg cggcagcgac cccgacacca ccacgagcag cgagaccagc ggcacggcca
     4861 ccacgggctc cgccaccgcc acgaccgggg agaacaccga cccgaccacc ggcgccaccg
     4921 gcaccggcac cggcgacacg agcacgagcg agcccggcac cacgggcacg agcgagcccg
     4981 gcaccacgga cgtggtgacc acgggcgaca cgagcacggg cggccccgac acgactgtca
     5041 cgagcgacac gggcgacacg agcacgggcg ggggcgacgg cctcggccag gcggcgcagg
     5101 acgccgcggc ggcgctcgag ctcgcggtcg acggcgtgtt gtatctcagc gagtcggagg
     5161 acacgtggac cgtgttcgcg ctcgcgggcg cggccccggt gaccgacaag aacgtcaagg
     5221 acgtcatcgc cgggatctat gtcccccacg acgatgtgcc gctggccgac cgcgtggtcg
     5281 agctgcgcac gctggcccag ctgatggacc cgttgacggt gatgcaggac tggtggggcg
     5341 acttcgagaa gatgcaggcc gaggactaca agcagatccg cgcgatcttc gagggccagc
     5401 tgagcaaccc gcaggtgttt cgcctcggtg agcagtccgg caacgacctc gtcggccagg
     5461 tcgacgtgtt cgtgatcggt gagaccgtcg acggcgacct cgtcggtatg tttgcgatcg
     5521 cggtggagac ctgatgcgcg cgccactgct caccctgtgc atgctgctcg cctgcaacgc
     5581 cggcggcggc gatacggact ccgacaccgg cgacaccggc gaggccggcc tggcgccggt
     5641 gcgcgtgttc gcggtcggca accatcacgg cctcgactat gtcgacagct acgcggcctt
     5701 cgaggccgag atgcagcgcc tgatggcggt cgtgcagccg aacctttcga aggaccaccc
     5761 caacctcgtg gtgttcggcg aggatgtcgg cctgccggcg gccttcctcg gcgcgcgtgg
     5821 tgcctcggcc cgcgcctcga ccacggcgca ggccggctac ctcgcggtcg cgatcgccta
     5881 cgaggacgcg gtcgtgcact acacccaggt ctacccgcac atcactttta atcgggcgat
     5941 ctcgctggcg ctgaccgaca ccatggcgcg cgccttcttc ggcacgttcc caaaattggc
     6001 gaagcagcac ggcgtgtggc tgtcggcgtg cacgctgctc gcggacacga ccgagagcac
     6061 ggacccggcc gacatcgccg cgttcggcga ccccgagctg acggggcaga gctcggtgta
     6121 cttgccggtc gacgagcacg tctacaacgt ctgttacatc tgggacgacg cgggtgaggt
     6181 cgtcggcgcc tcgcgcaagg tcaacctggt cgcgctcgag ggcatggacc tgctcgacct
     6241 ctccccggaa attttagaga acgtcgaggc cttcgacacg ccgtttggcc gcgtggcgat
     6301 cgctatcagc ctcgacgcct tcgtgccggc ctacgtgcag cacatcaacg acctcggcgc
     6361 gcagatcgtc ctgcaaaacg acgccaaccc gggcgcgtgg gcgatcattc agccgcaggg
     6421 cgggcacgac ggtccgcccg acggctcgat ggtgtggcag cccgaggagt gggaggactc
     6481 gacgctgcgc atggtcaccg acgccgccta cccgaacatc gagttcaacg tgtgcccgat
     6541 gatcgtcggc aacctgttcg acatcccctt cgacggccag agctcgatca ccgcgcgcca
     6601 tccacccgcc gataatcctg cgcgcggtta catcggcaac gcgccgagcg accgcttcct
     6661 cgcgctcgca ccctgggcct tccccgaccc cggcgacgcc gacccgggcc tcacgctcga
     6721 gcagcgccgc gaggcgatgc tgttgctcgg cgagcagctg gcgcccggct ccgggcacgc
     6781 cagcgagaac aagtaccgcg aggacgtgat ctgggccgac atccaggtca tccaggtgtc
     6841 ggccccgccc gccccgtagg tcaggtcgct gccgcggtcg cctcgctggc cttgaggctg
     6901 gcgatgtcga tgacgaagcg gtagcggacg tcgctgcgca ccatgcggtc gtaggcggcg
     6961 ttgatgtcct tgatgtcgat cacctcgacg tcggaggcga cgttgtgggc ggcgcagaag
     7021 ttgagcatct cctgggtctc gcggatccca ccgatcatcg agccggcgag ccggcggcgg
     7081 ccgacgatca gcgggaaggc ggacagcggc gtcggctccg gggcgccgac gagcaccatc
     7141 gtgccgtcga ggcgcagcag gccgaggtac gcgttgtagt cgtgctgggc cgagaccgag
     7201 tcgaggatga agtcgaagcg gccggcgtgc tcggtgaaca cccccggatc tttggtgacg
     7261 atgaagtcgc tcgcgccgag gcgcatcgcg tcctcgcgct tgctctccga gtggctgagc
     7321 acggtgacgt gggcgcccat cgccgcggcg atcttgacac ccatgtggcc gagcccgccg
     7381 agcccgacga tggcgatctt gtcgccggcc ttgacgccga agtgcttgag cggcgagtac
     7441 atcgtgatgc ccgcgcacag cagcggcgcg gcgcgctcga gcgggatgcc ctccggcacg
     7501 cgcagcacgt agtcctggtg gacggtgatc cgcgtcgagt agccgccgtg ggtcggctcg
     7561 ccgctggcgc gctcgcggcc gttgtaggtg ttggtgcagc ccttcccgca gtactgctcc
     7621 tcgccggcgg tgcaggcctc gcactcgcgg cacgagtcga cgaagcagcc gacgccgacg
     7681 atgtcaccct cgcggaagtt gttgtgcacc tcgatgccga cctgcgcgac gcggccgacg
     7741 atctcgtgac cggggaccat cgggaagatg ccgttgcccc actcgtcgcg ggcctgatgc
     7801 acgtccgagt ggcagacgcc gcagtagaga atgtcgatca gcaggtcgtg cgggccgggc
     7861 tcgcggcggg agatcgtgtg cggcgcgagc ggggagcgtg gatcgggggc tgcgtaggcg
     7921 ggggtctcga gcatgggcca gacctagcat gggccccggg agtcaaccac gtgaccccgc
     7981 cgggtgcgcc acctgcgtac cgggcccggc cggccccgga gggccgcgac ccacaggccc
     8041 tggggccccc gtcgcccgcg gctgcgcctg gatcgccggt ccggcgcagt cgacgacgcg
     8101 cgatggttca tgaaacgtgg gcaggacgcc catgcaaacg atcatcgcgc aattcgagca
     8161 agtcatccac gccgagtcgg cggtccgcgc cctcctcgag cgtaagtacg accgggccga
     8221 catcagcttc atcgccaacg atgtcaagag cgacacggcc ctgggtgacg gcagcggcac
     8281 gccggcgggc gccggccagg cgtcgatcgg gctgctgctc ggcatcggca gcttcatgat
     8341 ccccggcgtc ggtccgctgc tcgccgccgg tccgctggcg gtcggcgtcg ccggtgcagc
     8401 gtcgggcgtc gcgcaaaagg acgcccactg gctcgacccg ctgctcggcg gctacggcat
     8461 ccccgaggcc tccgcgcggg cctacagcga gcgcctgcac cggggcggcg cgctgatcct
     8521 catccccgcg cgcgaggaag tctccgacaa catcatcggc gtgctcgagt cgagcggcgc
     8581 cgtgcaggtc gacacccacg cgcgcgaccc gaagtcctcc ggggcttgaa taccctacag
     8641 cgcgatcagc acgtcgaagc cacccgcgcc gcgcggcggc gcgtgcggac ccgtgaacca
     8701 cgccgacact gccacctgca ccgcgccctc gcgggtgacc aggcgggccc cgacgcccgc
     8761 gagcgcgtgc gcataggcag gctcgagcac cggcggcggg cgcacgggcc acacggccag
     8821 cagcgcgccg gtgtcgacgt cacgcagctg tacgtgcaac accggcggtc cgagccgcga
     8881 cctggtcaat cggacaggta gcagcaggcc ctcggcggtc cgcaccgggt cgccgacgca
     8941 gcgggcgcgc aggctccaca gtacgcggcc gtcgccggtg aggcggtgga cctgcgggag
     9001 gtagccggcg ccgctgcggc ggtgctcgcc ctcggtgaac agcagcagca ggtcgtcgac
     9061 gacgcgcaat tgtccgcgcg ggatcagggt cgggtggccg ccggtggcgt cgatctgcaa
     9121 cgtccagccg gggccagcga ccatgacaaa gcggtcgttt tcggcgaatt cgacggaatg
     9181 gggtgtgctc gccatgcggg aattctagcc gctgggcggg catcatgaca gtgtcacgcg
     9241 tgcagtaacg cggacaggtc ggcgcccggg gcctgctcga cgggctcctg gccggcgcgg
     9301 aacaggcggg cgccggcctc gccgcgcagg accaggcggt cgccctcgcg gcgcagcatc
     9361 gcgggctcgc gcaggccgag caccggctgg tggttgtgct cgtgaaactc gtggatccgg
     9421 gtctcgcggg tctcgccctt gtgagtcgag tgcgggtcgg ggtcgaggta gtgcgggttg
     9481 aagttgaagg gcaccagacc gagcgcgaca aagctcggcg gatacacgat cggcatgtcg
     9541 ttgctgctgc cgatcgtcaa gcccgcgagg ttggtgcccg cgctcgagcc gatgtacggc
     9601 atgccggcgt cgacccgctc gaacaacgtg tcgcggatgt cgagccggta gacctcggag
     9661 agcaggcgaa aggtgttgcc gccgccgacg aacaccgcct gcgcggccgc gaccgccgcc
     9721 ctggcgtcat cggggtcgtc cagggtgtgc agcccctcga ccgcgatccc cagcggcgcc
     9781 aggcggctgg ccaccttggc ggtgtaggcg tcgcggtcgt gcagcgcgaa gggcacgaac
     9841 agcagccgct tcaccgggcc gaggaagtcg agcagctcgg cctccgcgtg gtcgaggtag
     9901 ccgtggccgt gcacgttgga gctgctgagc aggaggagtc gggacatcga cgcgcagcat
     9961 agcggcggcg gcgcatcgag tcatctcacg ggtgaccggc cttgcccgcg cccggtcgat
    10021 tcgtggaagc tgcggcgcat ggcggacgtg tggccttacg aggtcgagcc cttccagtgt
    10081 caccgctgcg gcaactgctg ccgcggcgac ggctacgtgg tgatgacccc ggacgacgtc
    10141 gggcgggccg ccgcgcacct cgggcgcagc gaggccgagt tcctggccga gttctgcgcc
    10201 acgcagccgg acggcagcgt gcacttgaaa gaccagagcg acgcgctgcg gtcgtgtgtg
    10261 ttcctgacgg actacaacct gtgccgggtg cacgcggcca agccgacgca gtgcgagggc
    10321 ttcccgatga agtggcggcc gagcaacgtg ctcgagatct gcgccggcat gcgggcggcc
    10381 gcgggcctgc ccccgccgca gcggcgtggc gtcgggctgc gggcgcgcgg ttgagcgcga
    10441 ccgatttcgc tacagggagg gacccgggcg agcgcccggt gacctgtccc ttcggacagg
    10501 cgctcagcgg agcatacaat aataacattc gccctcgtcc cagaggaccc actggttgtc
    10561 gtcgcacagg ccgggcaggc tggtggtgag gctgacggcg tcgagcgggt tgccccggaa
    10621 gtcgacctcg acgcagtcgt ccgcccagaa cggcgcgccg aggatcgggt cgatcgtggt
    10681 gatcgcgttg tccgcgagct tgagcacgcg cagcttggtg tgtccggcga gcggcgccag
    10741 gtcggtgatc gcgttgcccc cggcgcgcag ggtcctcagt gtcacgggtg agatcgtggc
    10801 gaggtcggag atcccggtgt gctcgaggcc gaggtcggtc agctccgtca gcgccgtgag
    10861 ctccgagatc ccgctggcga gcgggttgtc gctgacatcg agctcggtga gggcagccag
    10921 cgcggcgagc ggcgcgacgt cggagatctg gttgacctcg aggtgcagcg agcgcagctc
    10981 gagcagcccg gcgagcggcg cgatcgtggt gatggcattc tccgcgaggt cgagggtccg
    11041 cagcgcgggg aggctggcga ccggtgtcac atcggcgatc gcgttgtgtc gcaggcgaag
    11101 cgtgacgagc tccggcatcg cggtgagcac gtcgaccgtg gcgatctggt tgcggtcgag
    11161 ctcgagctgg tagagctgcg tcatcccggc gagcgggccg agctcgatga tgtcgttgaa
    11221 cgacaggtcg agctcggtca gcccgaccgc gccggcgagc ggtccgaggt cggtcacgcc
    11281 cgcgccggcg agcgtgagct tcgcgaggtg cgggatctcg ccgagcccga cgaggtcgat
    11341 gggcgtcgtg ccggcggcgc tcaggcgcgt gagcgcgggg tggtccgcga gcggggcaaa
    11401 ggacacgacc ggatttttgt cgacgtcgag gtgccgcagg tccggcaacg cggcgatcgg
    11461 cgcgaggtcg acgatgttgc tgccacgcag gctcaggtgg cgcagcggga gaccgacgag
    11521 cgggccgagg tcgccgacgt cctgcaagtc gagctggacc gactcgagct cgccgaggcc
    11581 cgacagcggg ccgaggtcgg tgatcccacc cggcccgatg atcagcaggt tctcgaggtt
    11641 ggcgaagcac tcgagcccgg cgaggctgtc gacctcgggc tcgccgacga acgagagcgt
    11701 cgtggtggtg agcgcgagcg cgggcgggat cgggccggcc tcgaggtcga gctcgtcgag
    11761 aaggtgcatg aacaggccgg gatcggggat gacgatctcc gcgcagtcga ccggctcgcc
    11821 ggtgctcgtg tcggcggtcg tcgtggaggt gtgctcgcta gcgctcgtgc tgtcgggcgc
    11881 gccgctggcc tcgctcgtcg gctcgggcgc gctggtctgg ccggccgaga ccgaggtcga
    11941 gtcgtcggcg ccgctcgtgc tgcccgtgac cgtcacgccc gcgacgctgt caccgcaggc
    12001 gatgaggagg agcgacccca ggggtgatgt cagccagcca cgcgccaccg catgagttta
    12061 tcgcgcgccc tcggcggggt gccaggccac cgcgcgaagg tattgcgcac cgggcgggcg
    12121 cgtcggcgcg cagcgccatc gctccgggct ttcaggtcag gttgaacgag tagggcaggg
    12181 tcatcggctg gccgtcgcgg gtcttcggga acgcccaggt cctggccttg tcggtcacgc
    12241 aggtggtcag gccggggtcg ttgacggtgc tgccggactc ggtcttgacc tcgctgacct
    12301 tgccggtgcc gtcgatcgtg aactcgagca tcagcttgcc gccgaggtcg ggcttcttct
    12361 ccttcgccgc ggcgaagcag tcggtgacgt cgctggtctt ttggttcacg acgtcctcga
    12421 agacatcgac ggtcaggaac tcgggggcgc tgtcctcctc cggggccgcg tcgccgccgt
    12481 tgtcctcggg ctggctgacg tcgctgccgc tgtcgggggc cgtggtgtcg gaggacttct
    12541 tgcaagcggc cagggtcagg gcgcccgccg ccacgacgag caagggccag gagatcgatg
    12601 cacgggtcgt catgccggcg gttttatcat gcagcgcggc gtcgatgccc gcgtgcatcc
    12661 ccgcacccgc tgtcatgatc gtcgcgactt tcgtcggcgc gacattgaac atgctcgctg
    12721 tgatacgcgt gaacagcgtg aaccactgat tacacgtagt ggcccgcggt tgttgtaaat
    12781 ggctttgctg aggcatgctc gcggtccgtt ggcccggtct cgcgccagcg ctcgcacact
    12841 ctccttcagc gccgatggcc tgaagccgct ttacggagcc agcgatgggc cttgaactca
    12901 gccttgatga catccgccgc gcgtgggacg cccgcgatcc ggacctggcc aacctcatcg
    12961 tcacgctcgt ggcggctccg gagccgaagc cgaccacgcc ggtacgcgag ggtgcgctga
    13021 gcttcgccag cttcacggcg cagctcgcca cggggaagtt tcgcaagagc acgccgcagg
    13081 agcgggcccg ctatcgcatc gacacgatgc gcgcgctcga ggtcgcgaac gcagaggtcc
    13141 cgctgccgcc gaggttgcag ctgcactcgg tgatcgtcga gctctggcag aaaaatggcg
    13201 cgtacgagcg cgccgtcttg ctcgagctga tccgcaaggt gccgctgtgc tggggcccgt
    13261 ggcgcgggct caagcgggtg ttcaaggagg ccgaggagcg caacgacacg gaggtcctcg
    13321 gcgcgctcgc ggctcgcctc gacgccgcct acgcgaccaa ggccagcagc tatcgcgaga
    13381 tctcgcggcg cacggtcggc tacctggtgc gtcgcgcgtg gcggtacctg cgacgcatgg
    13441 gccaaacgct cgcgcccgcc tacgtcgacg cggccgtcga cttcctgcgc gcctacgacg
    13501 acagcgtcgc gttcacgcgg acgtgggtct acaaccacat cctttcgcac gagacgcaca
    13561 agtacacgcg ccgcaacttc aagctgcacc gctccgacaa gccggcgctg accgagcgcg
    13621 cctacgccga gctgtggcgc cggacgccgc ggccggtgct ggcgctgctc gagcgcgcga
    13681 gctcggagca ggtccgcaag ttcgccgtgt cggcgctcaa gaccgacttt cgcgcgagcc
    13741 tgcgcgaggt cgaggccccg tgggtcgtgc gcctgatctc ggtgcagtcg gcgaccgtcg
    13801 acgagttcgt gatctggctg ctgtcgaacg tgcctcgctt cgagcagggc gggttccgcg
    13861 agctcggcct gcacgagccg gtgctgcgcc tgctcgactc gagcaacgac gccgcccgcg
    13921 cgtacgcggc cgcgtacgcc cgcacgcacg cgcgggacct cgagctcggc cggctgctgc
    13981 gcctcgccaa caacagccac gccgaggtgc gcaagctggc ggccgacctg ctcggcgacc
    14041 gtgacccgcg caaggacatc ggtctcgacg cgtggggtca gctgctcggc acgcagtacg
    14101 gacacgacct cgccgcgact gccctgcgca agcacttcag cgcgcgcgag ctgacgccgg
    14161 cgtggttcgc ggatcggctg ctcgacgagc gcggcaaggt gttcgagttc gccagcgacc
    14221 tgctgacgaa ggtgcacacg cacaagtcac tcggcgccga gttcttccgc ggtctattgg
    14281 atgacccgcg ggtctccgcg caggccgcca agttcgcgct cgacgccctc gcgcggttcc
    14341 ccggcgagga cctcggcaag gagttcctcg ggcgcatgat cttgcacccg aacactcgcc
    14401 cggcgctgct cgagtggatc accgaggagc gcatcaaagc cgaggacctc ggcgccgagt
    14461 tctggcgcgc cgtcgcgttt cggcccagtt gggacgccga tccctggatc aaggagctga
    14521 tcgccagcgg ccggccgtgg gcgcgggagc tccagttcag cgaggagctc gcccgcaccg
    14581 cgctcggctt cctgtccgac gtgcgccgct tctcgcccgg cgcgctcggc ttcgactggc
    14641 tgatgcagct cgcgctgcgc cccgagccgc actaccgcga gttcgcgctc gagtacatga
    14701 ccaaggcgct ggtgcccgcc gacttcgctc cgaaggtgga agcaccgaag cagagcaagg
    14761 ccgcggcgcc aaagacgatc gacctcggcg gcaagagctt cctgttcacc ggcaagctgg
    14821 cgacgatggt ccgcgacgag gcggaggcca aggtcaccgg cgcgggcggc aagaactcga
    14881 gctcggtgac cgcgaagctc gactacctcg tgatcggcga cgagggctcg ccgctgtacg
    14941 gcggcggtcg caagggcagt aagcaggtcg ccgcggagaa gctggtcgag aagggcgcgg
    15001 ggatcaagat catctccgag accgccttcc tacaaatgct ggccggtgag cagcgcgagt
    15061 tcacggctga ctcggtgacg gccggctgcg accgcctgtg ggagatggcg accggccccg
    15121 gccccgccga cgcgccgcag gcgagcttcg cccggcacta catccggcgg catcacccgg
    15181 acatcagcct cgcgttgacc gaccgcccgg tcgatcccgg cgccgagatc ccgacgagtt
    15241 atctcagctg ggcgcgcgtc aagccgttgt tggtcgacgc gcgcgcgccg gtgcggcagt
    15301 tcggcgtcga gctggcgcgc tgggagctgg cgcgctgggc cccgccgatc aacgagatcg
    15361 tcgaggtcac cgaggcgccg taccccgagg tccgcgagtt ctttgcaaag gcgctgctcg
    15421 ccgacgagtc ggtcgagcac cgccgctacc gcgtcgaccc ggcctcgctg accgcgtcgg
    15481 ccgtatacgg cttctgcgag agcctggtgt cggagacgcg cgggctcggc atgcagctga
    15541 tccaccgtta tcccaagctg gcgatccccg aggagctgtt ccggctcagc gagtcgccgg
    15601 accgccaggt gaccgccttc gtggtgcgca cgatctggtc gctgtatcac gagcgcggga
    15661 tcaccgaggg ctggaagccg gcgcctcgtg ctcccaagga cgccggtaag ccgggcaaga
    15721 agacggaagt gaagggcccg gcggtcccgg tcgagggcac gggcgccccg gcgcgccctg
    15781 aaaaaccacc ggcctccggc gaggcgctgc aggcgttcat gcgacggatc ctgttctcga
    15841 tcccgccgac gaagctgccg gtcgaggcgg cgcgcgccgc cgaggcgcag gtcgagggcg
    15901 cgccgcagcc ggacgagccg aaggccaagc gccgcgcgcg accgctgccg gcccgcaagg
    15961 ccaagctgtc gctgatcgag gcgatgcgtg acctggcgat cgacgaccgc gggttcgcgg
    16021 gcctggccgc gccgctgctg cgcgagttca tgggctcgcg cggggccagc gagcgcgcgg
    16081 catgcatggt ggcgctggcc cgcatcggcg gcgcccaccc ggatctcaag gtgctgacgg
    16141 aggacgctgc ctgatgtcga ccgcgaccac gagcgacaag ttcacgagcg acaagtacac
    16201 gcagatccgc ggcgagctgc gggccctgca cgcgcagggc agcgatctct ggatcacgac
    16261 cacgcacccg cagggcgccg cgacggcgct gtacaagctc aacctcgatt cgggcgactt
    16321 gcaggtgtcg ccgctcggcg gtggtgccgt ggcgctgatc gccgacgaca gccacctgta
    16381 cctcgctggc accgatgggc acatttataa gggcggcctc gacggcaagg cggtcaaggc
    16441 gatcggcccc aagctcgagc cggcgccgac ggccctcgcg ctggccgtca aggaccgcct
    16501 cgcggtcgtc tgcggcaagg agctggtgat cgtcgcccgc aaggacggca aggagatcca
    16561 gcgcctgccg ctcaacgagc tcggctcggc gatcgcctcc gacccgagcg gcgtgtggct
    16621 ggtcgtcggt ggtgaccgcg gctcgctgag cgtgttcgac tgcgaggaca aggaccgcta
    16681 cttcgcggcc gagtcgaaga agttgcacga gggcgcggtc accgggctgc tgttcgagct
    16741 cgaagaattg cgcgtgatgt cggtcggcaa cgacaaccgc ctgctgacga cgcacgtgcg
    16801 cgggcagctc gaggccgagg accgcggcgg caagaacggc cacagcgacc gcacgaccgc
    16861 gctgatcccc gggatcgccg acaagtttta tacggcgggc ctcgactcga cgatcaagac
    16921 gtggacccgt ggtagcgggc agcgcaggcc gtcgacgcaa aaggacgggc tcgccaaggt
    16981 ccatgcgctc gcgctcgcgg agctcaaggg ccggccgtgc ct
//
LOCUS       DASWNJ010000085         2960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_354848, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000085 DASWNJ010000000
VERSION     DASWNJ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354848"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1440
                     /locus_tag="VGB85_01600"
     CDS             <1..1440
                     /locus_tag="VGB85_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132733.1"
                     /translation="LAKNFRFTGFAPLLFRHQKCGFREEDDDRCRYTLWGSVPLFLAG
                     SDGKGRRTHSALSLYYFDRDKGGFKFYTWLGGAAVRPGERLTWYALNVGRTVTRTHAT
                     TVVFPLFFRKAHRTEDRSTTLVVPPLYIGRRKEDYTWFEAGLVFWNFRRPHKVTTAIL
                     PPLFYTSHSYAERRLTWTIPFFLRDNNWAKDRTLTIVPPGLVVQRRKGEDNDWVQFPL
                     LWHIERGKNSGTVGAAVWWDIRRKNTITQVIPALLVRHVKKDKELVVVGPGLGWWTRT
                     SGKVNGLHWRALFGIFGGGNESGQRYMSLFGGKIALKPKPLYESKRAKIRLMKQRKAD
                     ADAKVRADERAKDEAEKRAGAPAGTSVVPEHSQPAPAPETRRNLPEEPPATPAPTASP
                     WSGAAPTGGRPGVNSSVEPPAPSSAVPPSTPGGTTTPPPAAPPAAAPGTASSTTPNGP
                     ATRPGAAGTTTTPGATTPSTRPATTTPGR"
     gene            complement(1600..2049)
                     /locus_tag="VGB85_01605"
     CDS             complement(1600..2049)
                     /locus_tag="VGB85_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY0132734.1"
                     /translation="MPAVADHYAILGVSPSATTAEIRQAYRRRVALEHADRHAGDPRA
                     LERTRALNLARDVLLDPVRRARLDHFELARDPAPTRDPLVDTVARTFGAAPPPVARPS
                     RAVRIDPAPGWLRGAGLGLLAAVTALGVGIGVGAAIQTANRERESDR"
     gene            2191..2520
                     /locus_tag="VGB85_01610"
     CDS             2191..2520
                     /locus_tag="VGB85_01610"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEY0132735.1"
                     /translation="MSRYNCVQCGACCCNPPENRAEGFTDYVEVDARAPLLKKPELVR
                     RLVVYSDEGTPHLRLHPDGRCLALRGRVGQNVRCTIYADRPLPCRKVEAGSALCQRYR
                     RSHGLRP"
     gene            complement(2591..>2960)
                     /locus_tag="VGB85_01615"
     CDS             complement(2591..>2960)
                     /locus_tag="VGB85_01615"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY0132736.1"
                     /translation="IPIIIVALVSNEEGLSFGPPLLMLAWIAGIHLLEANIFNPKIIG
                     DAAHMHPVVVIFALLAGEHVYGLTGALLAAPVASMLQTVFLFAYRRSRWHAPGNLRVE
                     DLPAVPVDQSSASMSTATLR"
BASE COUNT          443 a         1074 c         1069 g          374 t
ORIGIN      
        1 ctggccaaga actttcgctt caccggcttc gcgccgctgc tgtttaggca ccaaaagtgc
       61 ggcttccgcg aggaagatga cgatcgctgc cgatacacgc tgtggggcag cgtcccgctg
      121 ttcctcgctg gatccgacgg caagggccgg cgcacgcact cggcgctgtc gctgtactac
      181 ttcgaccgcg acaagggtgg attcaagttc tacacctggc tcggcggcgc ggcggtgcgt
      241 ccgggcgagc gcctgacctg gtacgcgctc aacgtcgggc gcacggtcac gcgcacgcac
      301 gcgacgacgg tcgtgttccc gctgttcttc cgcaaggcgc accgcaccga ggaccgcagc
      361 accacgctgg tcgtgccgcc gctgtacatc ggccggcgca aggaggacta cacgtggttc
      421 gaggccggcc tggtgttctg gaacttccgg cgcccgcaca aggtgacgac ggcgatcttg
      481 ccgccgctgt tctacacgtc gcacagctac gccgagcggc gcctgacctg gacgatcccg
      541 ttcttcctgc gtgacaacaa ctgggccaag gatcgcacgc tgacgatcgt gccgccgggg
      601 ctggtggtcc agcgccgcaa gggcgaggac aacgactggg tgcagttccc gctgctgtgg
      661 cacatcgagc gcggcaagaa ttcgggcacc gtcggcgcgg cggtgtggtg ggacatccgc
      721 cgcaagaaca cgatcacgca ggtgatcccg gcgttgttgg ttcgccacgt gaaaaaggac
      781 aaggagctcg tcgtcgtcgg ccccggcctc ggctggtgga cgcgcacctc gggcaaggtc
      841 aacggcctgc actggcgcgc gctgttcggg atcttcggcg gcggcaacga gtcgggccag
      901 cgctacatgt cgctgttcgg cggcaagatc gcgctcaagc cgaagccgct gtacgagtcg
      961 aagcgcgcca agatccgcct gatgaagcag cgcaaggccg acgccgacgc caaggtccgc
     1021 gccgacgagc gggccaagga cgaggcggag aagcgggccg gcgcgcccgc gggcaccagc
     1081 gtcgtgcccg agcattcgca gcctgccccc gcccccgaga cgcgcaggaa cctgccggaa
     1141 gagccgccgg cgacccccgc cccgaccgcc tcgccgtggt ccggtgcggc gcccaccggt
     1201 ggtcggcccg gcgtcaactc ctcggtcgag ccgcccgcgc cctcgtcggc ggtgcccccg
     1261 agcacgcccg gcggcacgac gaccccgccc ccggccgcgc cccccgcggc cgccccgggc
     1321 accgcgtcga gcacgacccc gaacggcccc gcgacccgcc cgggcgcggc aggcacgacg
     1381 acgacgcccg gcgcgaccac ccccagcacc aggcccgcca cgacgacgcc cggtcgctaa
     1441 gaaaacctgt cctgcgggac aggtcccccg cacctggccg agccgtcacg aatgaaccag
     1501 tcacctgtcc cgcgggagag atgactcgcg ccgtcacacg ggtcgcgcgg gacagatgac
     1561 tcgcgccgtc acacgggtcg cacggggctg ctccgacgct cagcggtcgg actcgcgttc
     1621 gcggttcgcg gtctggatcg ccgcgccgac gccgatgccg acgccgagcg cggtcacggc
     1681 ggcgagcagg ccgaggcccg cgccgcgcag ccagccgggg gccggatcga tccggacggc
     1741 ccgggacggg cgagcgacgg gcggcggggc ggcgccaaag gtgcgggcca ccgtgtcgac
     1801 cagcggatcg cgggtcggcg cggggtcgcg ggccagctcg aagtggtcga ggcgggcgcg
     1861 acggaccggg tcgagcagga catcgcgggc gaggttgagg gcccgcgtgc gctcgagggc
     1921 tcgcgggtcc ccggcgtgac ggtcggcgtg ctcgagggcg acgcgccgcc ggtaggcctg
     1981 acggatctcc gcggtggtgg ccgacgggga caccccgagg atcgcgtagt ggtcggcgac
     2041 ggcgggcatg acggcaagca tgccacaggc ccggcgtcgt gcaccgcggg cgtccggcgg
     2101 ccgttcggcc gcggtcacgg gtcggatctg cgggggccgg gggacccgga gcgcggccgc
     2161 caccgggccg ccatgggtta tggtgcgccc gtgtcgcgct acaactgcgt ccagtgcggc
     2221 gcctgctgct gcaacccgcc ggagaaccgg gcggagggct tcacggacta cgtcgaggtc
     2281 gacgcccgcg cgccgctgct gaaaaagccc gagctcgtgc gccggcttgt cgtctactcc
     2341 gacgagggca cgccgcacct gcgcctgcac cccgacggcc gctgcctggc cctgcgcggc
     2401 cgcgtgggcc aaaacgtgcg ctgcacgatc tacgccgatc ggccgctgcc gtgccgcaag
     2461 gtcgaggccg ggagcgcgct gtgtcagcgc taccggcgaa gccacggcct gcgcccatga
     2521 tcatgacccc gacccctcgg gcatgatgtc gggatgcgcc gactcgcgtc ctcgtgctgc
     2581 ccgcgccagg ctagcgtagc gtcgccgtgc tcatcgaagc cgagctctga tccaccggca
     2641 ccgccggcag atcctcgacc cgcaggttcc cgggtgcatg ccaccgcgag cgccggtacg
     2701 cgaacaggaa caccgtctgc agcatgctcg ccaccggcgc ggccagcagc gcccccgtca
     2761 gcccgtacac gtgctcgccg gccagcagcg cgaagatgac gacgaccggg tgcatgtgcg
     2821 ccgcgtcgcc gatgatcttc gggttgaaga tgttggcctc gagcaggtgg atgccggcga
     2881 tccacgcgag catcagcagc ggcgggccga acgagaggcc ctcctcgttg gacaccaggg
     2941 cgacgatgat gatcgggatc
//
LOCUS       DASWNJ010000086         4507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_425709, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000086 DASWNJ010000000
VERSION     DASWNJ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425709"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..1303)
                     /locus_tag="VGB85_01620"
     CDS             complement(239..1303)
                     /locus_tag="VGB85_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132737.1"
                     /translation="MRARYVPMMLVVALIAAGFYCYGLGSGGTFYHRLVRAPFAPAID
                     APYYRASVLHVMLGHVLGLGGSILAFRLAVLACLWAALFYLAGVMRGTLAWTDGCLVL
                     LVLMFHPSAMIAYAWTCHPDAVTYLLTAVLLFSRRPWVLAAVAALGAWNHLAMWAIVC
                     VEAALLWWAFIGAQAKRPVIATAAGLVVGAVSCKLTLMLCGVEIVNDRLALAWGQEFA
                     ALVGYWTLAGWPILYTLYFAHIVWVPIVMIRLYRERPRLMWVFLATQLLALAAACCAQ
                     DTTRVFAFLAWGPLVYCLVHVLAARSGRAGLVLRIVVGAAVVATIVGPKIFAWKGDIR
                     DTEGARAHLRAVWASASRAR"
     gene            complement(1305..2231)
                     /locus_tag="VGB85_01625"
     CDS             complement(1305..2231)
                     /locus_tag="VGB85_01625"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0132738.1"
                     /translation="MAGAHPRDSLTPRQATALVALGAAVISVTALFVKLISLGPTAIG
                     AYRCLFAAAALCAWVAVPRRSAVVQPGGVSGRAILFAVGAGACYAGDLYVWHKSILLS
                     GTGLATLLANTQVFWVAVLSALLLGERLSWRLAVCAVLALLGVVLLAIPGLSASPVHL
                     QGILLGLATGVFYAGYYLCLRASQSAPRRLSLAANLGVTCVVAALLLFITAAITGEPV
                     PWPDTRDLGLLLLLGVGAHVGGWVLISRGMTGVPAATGSLLLLLQPVLATIWGVLWFT
                     EPFGGYEIAGTLLSLLAIYAAMVTRIVPDDGD"
     gene            2369..2902
                     /locus_tag="VGB85_01630"
     CDS             2369..2902
                     /locus_tag="VGB85_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0132739.1"
                     /translation="MLLLRPATLHDLPALHAIERSATELYYEEGFSPAQVDPRSEADI
                     RLLLKYTTVLLACEDDEPIGYASFYTRGPFMHLEEIAVRRDRQRRGCGKALAQQALAE
                     AKADPQCTHLSLVTFSSARWAVDMYSNLGFRFLTPGEDLPQSELLQEIVEIEAAACQT
                     CAPRVPMILAIRPPAAT"
     gene            complement(2911..3396)
                     /locus_tag="VGB85_01635"
     CDS             complement(2911..3396)
                     /locus_tag="VGB85_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132740.1"
                     /translation="MPVVFSVREMVVEGQLAGVRPGDSRGLVRAQLGAPDDWQADAAV
                     EVAAIWRYGNFEVHFDGDVVWMLFNDYLDELDAGPGRTLDPWILAGSVPGPDAVMQRL
                     RDDNVPFMVGRDVLDRRVVWIEGGACLAFDRDEDDGVEGWFAIELLSPSRAPNFTPEP
                     L"
     gene            3447..4049
                     /locus_tag="VGB85_01640"
     CDS             3447..4049
                     /locus_tag="VGB85_01640"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0132741.1"
                     /translation="MRRDSHEQNRRSWNLVIPAHDSHKRDQAGFLRAGGSTLFPDELA
                     LLGVTGVGGPGERVLADMTPETGPLAGLDLVHLQCNVGQDSLSLARLGARVTGVDISD
                     EAVARATQLAADAGVAASFVRADIYDWLADTAAGATRFDVAFATYGALPWLSDLDAWA
                     AGVAAVLRPGGRLVALEFHPIAFIFDEQLRPPSPTSAAAA"
     gene            4046..4363
                     /locus_tag="VGB85_01645"
     CDS             4046..4363
                     /locus_tag="VGB85_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132742.1"
                     /translation="MTITPEGVGDYVARSGDLLAPSGFEPGITDFRNPQPCHEFQWTI
                     ADWLGAILKAGLVLEQIRDYPYANGCQIFNNMRTLPGKRFAMPEGAPDLPLMCGFVAR
                     KPT"
BASE COUNT          747 a         1811 c         1396 g          553 t
ORIGIN      
        1 caggtcgatc gtcgcgtcgc ggtcgtcgtc gtcgctgatc ttcacctgca cgccgaggtg
       61 gatcagcgtc ggcgggtcga gcagcgtctc ctgcacgacc agcgcatccc cggcccacgc
      121 ccgccccgcc gacatccccg cgaccacccc cgccaccagc gcgccatgtc tcatgcgccg
      181 tagcatagcg ggatgcctcg cgccgcgtga tgtcggcgct cgcccccacg gctccccctc
      241 agcgcgcgcg cgatgcggag gcccacaccg cccgcaggtg ggccctggcg ccctcggtgt
      301 cgcggatgtc gcccttccac gcgaagatct tcggccccac gatcgtggcg accaccgccg
      361 cgccgaccac gatccgcaag accagacccg cgcgaccgct ccgcgccgcg agcacgtgca
      421 cgaggcaata taccagcggc ccccaggcga ggaacgcgaa cacgcgcgtc gtgtcctgcg
      481 cgcaacatgc agccgcgagc gcgagcagct gcgtcgccag gaagacccac atcagccgcg
      541 gccgctccct gtacagccgg atcatcacga tcggcaccca cacgatatgc gcgaagtaca
      601 atgtatacag gatcggccaa cctgccagcg tccagtagcc gacgagcgcc gcaaattcct
      661 gcccccacgc gagcgcgagt cgatcattta cgatctcgac cccacagagc atcaacgtca
      721 gcttgcagct caccgcgccg accaccagcc cggccgccgt cgcgatcacc ggccgcttcg
      781 cctgcgcccc gatgaacgcc caccacagca gcgccgcctc gacgcagacg atcgcccaca
      841 tcgccaggtg attccacgcc ccgagcgccg cgacggccgc cagcacccac gggcgccgcg
      901 agaacagcag cacggcggtc agcaggtacg tgaccgcgtc cgggtggcac gtccacgcat
      961 aggcgatcat cgccgagggg tgaaacatca gcaccagcag caccagacaa ccatcggtcc
     1021 atgccagcgt accgcgcatc acccccgcca ggtagaacag cgccgcccac aagcacgcca
     1081 gcaccgcgag acgaaacgcc aggatcgacc cgccgagccc gagcacgtgc ccgagcatca
     1141 catgcagcac cgaggcacga tagtacggcg cgtcgatcgc gggcgcgaac ggggcccgga
     1201 ccagccggtg ataaaacgtc ccgcccgacc cgagcccata acaatagaac ccggcggcga
     1261 tcagcgcgac gacgagcatc atcggcacat atcgcgcgcg catatcaatc tccgtcgtcg
     1321 ggcacgatgc gcgtgaccat cgccgcgtag atcgccagca gcgacagcag cgtgcctgcg
     1381 atctcgtagc cgccgaacgg ctcggtgaac cacagcaccc cccagatcgt cgccagcacc
     1441 ggctgaagca gcagcagcag cgaccccgtc gcggccggca ctcccgtcat gccccgcgag
     1501 atcagcaccc agccgccgac gtgcgcgccg acccccagca atagcaacag gccgaggtcg
     1561 cgggtgtcgg gccacgggac cggctcgccg gtgatcgccg cggtgatgaa cagcagcagc
     1621 gccgcgacca cgcaggtcac cccgaggttg gcggccagcg acagccgccg cggcgcgctc
     1681 tggctcgcgc gcaggcacag ataatagccg gcgtagaaca cccccgtcgc cagcccgagc
     1741 aagatccctt gcaggtgcac cgggctggcc gacagcccgg ggatcgccag cagcaccacg
     1801 ccgagcagcg ccagcacggc gcacaccgcc aggcgccagc tcagccgctc gccgagcagc
     1861 agcgccgaca gcacggccac ccagaacacc tgcgtattgg cgagcagcgt cgccaggccg
     1921 gtgcccgaca gcaggatgct cttgtgccag acgtacaggt cccccgcgta gcaggccccc
     1981 gcgccgaccg cgaacaggat cgcgcgcccc gagacccccc cgggctgcac cacggccgat
     2041 cggcgcggca cggccaccca ggcgcacagg gctgccgccg cgaacaggca gcgatacgcc
     2101 ccgatcgccg tcggcccgag cgagatcagc ttcacgaaca gcgccgtcac gctgatcacc
     2161 gccgcgccga gggcgaccag cgcggtcgcc tgccgcggcg tgagcgagtc tcgcgggtgc
     2221 gcgccggcca ccggctgcgt ttacacccgc gctcgcgccc ggtccaggcc gccaggcccc
     2281 cgccaggacg gccagggtcg gcttttgtgc gggcgccggc cgcggcgctc cccggcgccg
     2341 cggcgacgcg ataccctgcg gccccgcgat gttgctgctt cgccccgcca ccctgcacga
     2401 cctgcccgcg ctgcacgcca tcgagcgctc ggcgaccgag ctttattacg aggagggctt
     2461 ctcgccggcc caggtcgacc cgcgcagcga ggccgacatt cgcctgctgc tcaagtacac
     2521 cacggtgctg ctcgcctgcg aggacgacga gccgatcggc tacgccagct tctacacgcg
     2581 cggcccgttc atgcacctcg aggagatcgc cgtccgccgc gaccgccagc gccggggctg
     2641 cggcaaggcc ctcgcgcagc aggccctcgc cgaggccaag gccgacccgc agtgcaccca
     2701 cctctcgctc gtcaccttct cgagcgcgcg ctgggccgtc gacatgtaca gcaacctcgg
     2761 ctttcgcttc ctgacccccg gcgaggatct gccgcagagc gagctgctgc aggagatcgt
     2821 cgagatcgaa gccgccgcct gtcagacctg cgcgccgcgc gtgccgatga tcctcgccat
     2881 ccgcccgccc gccgcgacct gaccgggcgg tcagagcggc tcgggcgtga agttcggcgc
     2941 ccgcgacggt gacagcagct cgatcgcgaa ccagccctcc acgccgtcgt cctcgtcgcg
     3001 gtcgaatgcc aggcacgccc cgccttcgat ccacaccacc cgacggtcga gcacgtcgcg
     3061 cccgaccatg aagggcacgt tgtcgtcgcg cagccgctgc atcaccgcgt ccggccccgg
     3121 caccgaaccc gcgaggatcc acgggtcgag cgtgcgcccc gggccggcgt cgagctcgtc
     3181 gagataatca ttgaacagca tccacacgac gtcgccgtcg aagtggacct cgaagttccc
     3241 gtagcgccag atcgccgcga cctcgaccgc cgcatccgcc tgccagtcat cgggcgcgcc
     3301 cagctgcgcg cgcaccagcc cgcgggaatc tccgggtcgg acgcccgcga gctggccctc
     3361 gaccaccatc tcccgcaccg agaacacgac cggcatcccc ggcatcctac caccccgccg
     3421 accccgatcg cgctaccctg tgccgcgtgc gacgcgactc ccacgagcag aaccgccggt
     3481 cctggaacct cgtcattccg gcccacgaca gccacaagcg cgaccaggcc ggcttcctgc
     3541 gcgccggcgg ctcgaccctg ttccccgacg agctcgcgct gctcggcgtg accggcgtcg
     3601 gcggcccggg cgagcgtgtc ctcgcggaca tgaccccgga gacaggcccg ctcgccggcc
     3661 tcgacctcgt gcatctgcaa tgcaacgtcg gccaggacag cctctcgctc gcgcgcctcg
     3721 gcgcccgggt gaccggcgtc gacatcagcg acgaggcggt cgcccgcgcc acccagctcg
     3781 ccgccgacgc cggggtcgcg gccagcttcg tgcgcgccga catctacgac tggctcgccg
     3841 acaccgccgc cggtgcgacc cgcttcgacg tcgccttcgc cacctacggc gcgctgccgt
     3901 ggctgtcgga cctcgacgcc tgggccgcgg gcgtcgccgc ggtgctgcgc cccggcggcc
     3961 gcctcgtcgc cctcgagttc cacccgatcg ccttcatctt cgacgagcag ctgcgcccgc
     4021 cttcccctac ttcggcagcg gccgcgtgac gatcaccccg gagggcgtcg gcgactacgt
     4081 cgcacgctcg ggcgatctat tggccccctc gggcttcgag ccgggcatca ccgacttcag
     4141 gaatccccag ccctgccacg aattccagtg gacgatcgcc gactggctcg gcgcgatcct
     4201 caaggccggc ctcgtgctcg agcagatccg cgactatcca tatgccaacg gctgccaaat
     4261 ttttaataat atgcggacat tgccaggcaa gcgctttgcc atgcccgaag gcgccccgga
     4321 cctgccgctg atgtgcggct tcgtcgcgcg caagccgaca taaaaaacgc cgagcgagcg
     4381 caactgagtc gcacgcgccc ggcgtctata tctactgtcc gcggattact tcgcggtgcg
     4441 cggcagcggc gggggcggcg gcacggcggt cgtcttgctg acggtggtcg ccgtctcgac
     4501 gcctgcg
//
LOCUS       DASWNJ010000087         3498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_319020, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000087 DASWNJ010000000
VERSION     DASWNJ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319020"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VGB85_01650"
     CDS             <1..442
                     /locus_tag="VGB85_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132743.1"
                     /translation="SPGEQPPASATDGFPLVVIDTPPEAGIPAIVTPDLWVCPVDNRT
                     AIEALATVVPTMAAKAPCYVVFYAADAGGVSTLRGLQEATKHIPQLEYHPEVIPHNPA
                     TFRAQAYYKPVWDVPYGKGTAAHHMMVRLCDAILERAGVSEGRR"
     gene            540..944
                     /locus_tag="VGB85_01655"
     CDS             540..944
                     /locus_tag="VGB85_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132744.1"
                     /translation="MKHENSTTEAPTEKPQSTPTQSARRPRRSSDDAGDDADAAPDAA
                     PRRAARSTARRSNASPDDEGTGETPRARAQGGQGRPPKEDRAQRVIRLTAQLDAKLRQ
                     LAEFRGVDLNAAASVAIAEDWRRCFGSPLDRT"
     gene            1806..2228
                     /locus_tag="VGB85_01660"
     CDS             1806..2228
                     /locus_tag="VGB85_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY0132745.1"
                     /translation="MRLLEIRSLALALLLACACSDPGGVEGVQVAVAGSARTWTHAEF
                     AAMPIASVTYHGKQYTGVPLPTLVSALGASADRPLTAVASDGYTQTLAPEILARTDAL
                     LAHTVDGAPLPASEGPLRLVVPGAKGLSVKQLVRLGQP"
     gene            complement(2242..>3498)
                     /locus_tag="VGB85_01665"
     CDS             complement(2242..>3498)
                     /locus_tag="VGB85_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY0132746.1"
                     /translation="RDLADRFVREYVDVYLKPGTAINYRKHIADHILPALGDRDFESV
                     TRNDVQALHASLKDVKASANYVVCVVGSLYSRIIDDWKLSTMNNPNHKIRLFKLKTRE
                     RFLTPEERQHVEATLLRGLQIPPGRKGHLDRMGVWALQLLSLTGLRRDEILTLTWPMV
                     DWQHRCLNLPETKTGQRSVPVPSQVMTLLRQIHDRTGNRKDGLVVRSRTGRKLSGLNL
                     TWKNIREAVGIPDVRLHDLRHSFASDALMGGVPLAIVGEMLGHRQPSTTKRYAHLANR
                     VVREALEHTAGIITAATNLPPALPVAPFEPLRDVQWAAIVTLVEADRPRGGKPVDLRQ
                     VVDGIRWVLHTQAHWSDIPATYAATSTCWRWYKRWCADGTWPKVEALIAAPLAKPSRR
                     PTPRALPVAPRGRARPARPSVPALSA"
BASE COUNT          525 a         1228 c         1258 g          487 t
ORIGIN      
        1 ctcgccaggg gagcagccgc cggccagtgc gacggacggc tttccgctcg tggtcatcga
       61 cacccctccc gaggcgggca tcccggccat cgtcaccccg gatctctggg tctgtcccgt
      121 cgacaaccgc accgcgatcg aggccctggc gacggtcgtg ccgacgatgg ccgcgaaggc
      181 gccgtgctac gtcgtcttct acgcggcgga tgcgggggga gtctccacgc tccgcggcct
      241 gcaggaagcc acaaagcaca tcccgcagct cgagtatcac ccggaggtca tcccccacaa
      301 ccccgcgacg ttccgggccc aggcgtatta caagccggtg tgggacgtgc cctacggcaa
      361 ggggacggcc gcccaccaca tgatggtccg gctctgcgac gcgatcctcg agcgggccgg
      421 cgtgtccgag ggaaggcgtt gatgggatgg ggcgtggtcc gtcgttcaac cccgtgccct
      481 tcgcgggcac ccacgcacca ccgggcgcca gcccggaggc gctccatgag ctcgagaaga
      541 tgaagcacga gaacagcacg accgaggcac cgaccgagaa gccgcagagc acaccgacgc
      601 agtcggctcg ccggcctcgc agatcgtccg acgacgcagg ggacgacgcc gatgcggccc
      661 ccgacgccgc gccgcgccgc gcagcgaggt cgacggcgcg ccgctcgaac gcatcacccg
      721 acgacgaggg cacgggcgag actccgcgcg cgcgcgcaca gggcggccaa ggccgaccgc
      781 cgaaggaaga ccgcgcgcaa cgggtcatcc gtctgacagc gcagctcgac gcgaagctgc
      841 ggcagctcgc cgagttccgc ggcgtggacc tgaacgccgc cgccagcgtg gcgatcgccg
      901 aagactggcg ccgttgtttc ggatcgccgc ttgatcgcac ctagacatca gtcagcgatc
      961 aggtcgcccg ccgcgcgctc ctgcgtcttg agcagcgcac aggtctcccc tatgctgttc
     1021 tgatggcggc tgacgggcgt ggggactcag atacggagaa ggcgatcgcc ggcatcgcga
     1081 tcggtgtggt cagcggcatc gcaggaggac tgatcatcgg tgggatcgtc gaccggctga
     1141 ggtccaaagc gcttcctgca ccgccgtctc gacgccaggt cgccagcacg ccggggcccc
     1201 tcccggtcga cggctggcga acgcccgtct ggccaacctc gcccgcgtgg acacgaccgc
     1261 ccggagcgtc cccccggcga gcagagcccc cgccagcgtc cccccggcga gcagagcccc
     1321 cgccagcgtc gccaaggcca aagcccgcag agccctcgcg ggagccccag gccacggagc
     1381 ccgcgtcccc aggcccaaag cccgcggagc cctcgcgggc ttccccgggc ccccaacctt
     1441 cggcgccgtc gcgggcgtcc cggggctcgc gacgtggccc ggtcttcatc ccaacgccga
     1501 tggtggagcc ggtgcccaag ccgagggcgg agtccgagcc ggacgtcgaa ccgtggatgg
     1561 aggacttcgc gcgtcaggtc tccgagggga ttaagcaagc cgcccggcgg tgatgaacga
     1621 cgaggccgcc tgcagcgaac ggagggccgt ccacagcgct tcgcagcgca tgggcggccc
     1681 ttctccgtgg gcgcggccgc ggaccacgta cggtgccgcg acgcagccgg ggcggggctc
     1741 gggcctcaga cgttcggcct ctgagggcgc caggaccgcg gcggacggtg tatggtagcg
     1801 acgcgatgcg tctcctcgaa attcgttctc tcgccctcgc gctgctgctc gcgtgcgcct
     1861 gctccgaccc tggcggcgtg gagggcgtcc aggtcgcggt ggccgggtcg gcgcgcacct
     1921 ggacgcacgc tgaattcgcc gcgatgccga tcgcgagcgt cacgtatcac ggcaagcagt
     1981 acaccggcgt cccgctaccc acgctggtca gcgcgctcgg ggcgtcggcg gatcggccgc
     2041 tgaccgcggt ggcgagcgac ggctacacgc agacccttgc accggagatc ctggctcgca
     2101 cggacgcgct tctagcccat acggtcgacg gggcgccgct gcctgcgagc gagggacccc
     2161 tgcgcttggt ggtaccgggc gcgaagggcc tgtcggtgaa gcaactggtg cgcctggggc
     2221 agccgtgacc cacgcgccgg gtcaggctga caacgcgggc acgctggggc gcgcgggccg
     2281 ggcccggcct cggggggcga ccggcagtgc ccgcggcgtg ggacggcgcg acggcttggc
     2341 cagcggcgcg gcaatcaggg cctcgacctt gggccaggtc ccgtcggcgc accagcgctt
     2401 gtaccagcgc cagcacgtcg acgtagcggc gtaggtcgcc gggatgtccg accagtgcgc
     2461 ttgcgtgtgg agcacccatc ggatgccgtc aacgacctgc cgcaggtcca cgggcttgcc
     2521 accgcgcggg cggtcggctt ccacgagggt cacgatcgcc gcccactgga cgtcgcgcag
     2581 cggctcgaac ggcgccacgg gcagcgcggg gggcaggttc gtcgccgcgg tgatgatgcc
     2641 ggcggtgtgc tcgagcgcct cgcggacgac gcggttggcc aggtgggcgt aacgcttggt
     2701 cgtgctcggc tggcggtgcc ccagcatctc gccgacgatc gccaggggca caccgcccat
     2761 gagcgcgtcg ctggcgaacg agtgccgcag atcgtggagc cggacgtcgg ggatgcccac
     2821 ggcctcgcgg atgttcttcc aggtcaggtt caggcccgag agcttccggc cggtgcgcga
     2881 gcggaccacg aggccgtcct tgcggttgcc cgtgcggtcg tggatctggc gcagcaaggt
     2941 catgacctgc gaggggacgg gcaccgaacg ctggcccgtc ttggtctccg ggaggttcag
     3001 gcaacggtgt tgccagtcga ccatgggcca agtgagggtc aggatctcgt cgcggcgtaa
     3061 gccggtgagc gacagcagtt gcagcgccca cacgcccatc cgatccaggt gtcccttgcg
     3121 gccgggcggg atctgcaagc cgcgcagcaa ggtcgcctcc acgtgctgcc gctcctcggg
     3181 cgtcaggaag cgctcgcgcg tcttcagctt gaacagccgg atcttgtggt tcgggttgtt
     3241 catcgtcgac agcttccagt cgtcgatgat gcggctgtaa agactgccca cgacacagac
     3301 gacgtagttg gccgaagcct tgacgtcctt caggctcgcg tgcagcgctt gcacgtcgtt
     3361 gcgggtcacg ctctcgaagt cacgatcgcc gagcgccggc aggatgtgat cggcgatgtg
     3421 cttgcggtag ttgatcgccg tgccgggctt cagatagacg tccacgtact cgcggacgaa
     3481 gcgatcggcg aggtcgcg
//
LOCUS       DASWNJ010000088         3774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_637953, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000088 DASWNJ010000000
VERSION     DASWNJ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637953"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VGB85_01670"
     CDS             complement(<1..228)
                     /locus_tag="VGB85_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132747.1"
                     /translation="MQRLYNHFFARLPVRPLANDGDLHILNAAEVRLIRRRHRQALLL
                     SAGLSVAGFLAYFLPVYSAPAMFPSAPVTLPI"
     gene            complement(325..1551)
                     /locus_tag="VGB85_01675"
     CDS             complement(325..1551)
                     /locus_tag="VGB85_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132748.1"
                     /translation="MLLALFLGCPHPSGPSSVTPRLDAARELERGPDPPPPLPPPRPA
                     AKSLAEGEPGLVVINPGDVDIDVYTVQVVDAAGLLGTRVLWPHRHDCDGHEHLTHIVP
                     KQGARFTLPPPTRTYTPGGCELGPPLPDGEYVVLVDSGYAAELHAAAPFSLPMRAPVE
                     LRIEPHVQAVPCTDALARRAANLVTRGAWPSFGMPAPIAARILDGCDLGRARCVTRGE
                     EPLRPPERCEITLFTHEYGALLRVARPAGSDALRGLTAMLDHDVVHSRAPQIDRTSSS
                     TYTVDGEQVVIAGMSSEHWHVHGGDAAGIGGVTLRVDNPLDRPVAYRVEGLEFLVSYS
                     CELPSEVRARPALLQVAPRDRLPPGSSALQLTFEAQSAYQGHCERFATRVILSVEGVK
                     RAVTSEHHVGRFDVAD"
     gene            2069..3772
                     /locus_tag="VGB85_01680"
     CDS             2069..3772
                     /locus_tag="VGB85_01680"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEY0132749.1"
                     /translation="MRTYHSTICTLLVAGLAATGGCYNGSGGGQTGDSAATEAGGTPG
                     DTGQQPTGSDAGDTDQPTGEPVDGDPLVYSGLRRLTADEYNLTVRDLLGLDAASQALL
                     PNDPLSPFDNDYRDQTASQALIDGADLLAADLATEALVDPVARTTLVGCTPAGPDDAV
                     CFDQFIASFGRRALRRTLEPADIERFSVFLDHSIEAGDFYVGVESALRAFLQHPEFLY
                     RVEIGEPVADEPGVYRLGSLEIAARLSYFLWGSTPPDWLLDQAEGAGLDTPEKIRAAA
                     QQLLADPRARARIARFHALWMGYITLPHGAELADAMKAETQALIDRVLFDEKGPWQDL
                     LRSEQTFVSNFLAGHYGLPLPGGDGPSWVDYGDSGRRGLLSHGSFLSVGAKFTDTSPT
                     QRGLLIRTRLFCQDIKLPEDSDVDVDNPPGDPNACKPQRYSEHASGKCASCHSLMDPI
                     GFGLENYDMAGRFREFEPDRPDCPISGEGELLGVGKFSGPAELGELMLGVPDLNHCVL
                     TQLYRFAMGRYELHEQDTLAIQRLLKGVPVGGDLPFEATLLEFIDTPAFRHRREEPTD
                     I"
BASE COUNT          540 a         1367 c         1348 g          519 t
ORIGIN      
        1 gatcggcagg gtcaccggcg ccgacgggaa catcgccggc gcgctgtaga ccggcaggaa
       61 gtacgcgagg aagcccgcga cgctgagccc cgccgacagc agcagcgcct ggcgatgccg
      121 gcgacggatc aggcgcacct cggccgcgtt gaggatgtgc aggtcgccgt cgttggcgag
      181 cggccgcacc ggcaggcgcg caaagaagtg gttgtagagg cgttgcatcg ctcgccagcg
      241 ccgcggtcat accaccggag ctgtccgcgg tcgagcctgc gcgcggccgg ctcgcggccg
      301 cgccgggccg tcacccgcga aacctcagtc tgcgacgtcg aatcgcccga cgtggtgctc
      361 cgacgtgacc gcccgcttga ccccttcgac gctgaggatg acccgcgtgg cgaagcgctc
      421 acagtgcccc tggtaggcgc tctgtgcttc gaaggtaagc tgcagcgcgc tcgacccggg
      481 aggtagccga tcgcgcggcg cgacctggag gagcgcgggg cgagccctga cctcggaggg
      541 cagctcgcag gaataactga ccaggaactc gaggccctcg acccgatacg cgacgggtcg
      601 atcgaggggg ttgtcgacgc gaagcgtgac gccgccgatc cccgccgcgt caccgccgtg
      661 cacgtgccag tgctcgctgc tcatgccggc gatgacgacc tgctcgccgt cgaccgtgta
      721 ggtgctgctg ctggtccggt cgatctgcgg cgcccggctg tggacgacgt cgtggtcgag
      781 catggccgtc agaccgcgca gggcgtcgct cccggcgggc ctcgccaccc gcaggagggc
      841 cccgtattcg tgggtgaaga gggtgatctc gcagcgctcc ggaggccgca gcggttcctc
      901 tccgcgggtg acgcagcgcg ctcgaccgag gtcgcagccg tcgaggatgc gggcggcgat
      961 cggcgctggc atcccgaacg atggccacgc gccgcgcgtg acgaggttgg ccgcgcgacg
     1021 agcgagggcg tcggtgcagg gcaccgcctg cacgtgcggc tcgatgcgca gctcgaccgg
     1081 cgctcgcatc ggcagggaga acggcgccgc ggcgtgcagc tcggccgcgt agccgctgtc
     1141 gacgagcacc acgtactccc cgtcgggcaa cggcgggccg agctcgcacc cgcccggcgt
     1201 gtacgtgcgc gtcggcggcg gcagcgtgaa ccgagcgccc tgcttcggca cgatgtgcgt
     1261 caggtgctcg tgcccgtcgc agtcgtggcg gtgcggccag agcacgcggg tgccgaggag
     1321 cccggccgcg tcgaccacct ggaccgtgta gacgtcgatg tcgacgtcgc cagggttgat
     1381 gacgacgagg cccggctcgc cctcggccag cgacttcgcg gccggccgcg gtggcggcag
     1441 aggtggcggc gggtcgggcc cgcgctcgag ctcgcgcgcg gcgtcgagcc tgggcgtcac
     1501 ggagcttggc ccggacgggt gcgggcaccc gaggaatagc gccaggagca ccgcggaggc
     1561 aggtgctgcg ccgggttgga gagcgcgcgt catggctatc ggcacgggtg agtctcgcag
     1621 gaggctgacc tggaacgcgt gtgccccgga tccggcgtga ggacatgctc cgcgcgccgt
     1681 ccccttcaga gggggatcgc gcagacaccg acgttcatgt actcgggcgg cgccatgccc
     1741 tccgcgaaca tcgcattcgc agacgagcac tacggcgagc tcgcgtgctt cgcggacgcg
     1801 acgctcgggc ccgagctcgc ggccccggca aaatgccgtg ccacgacgtt ccacgcggtc
     1861 gcccccaggc ggtgtctcgc cgcccccggt gctgcgcagc cccacgcggg gcggcgctcc
     1921 cccgtccgct gtcacggccg accgcacgcg atatcgcggt ccctcgcgcc tgtgcgcccc
     1981 ggcttgcgat atcaaggcac ggtcaagtcg cgtcacgctc cggccgcctc ggcgccgggc
     2041 gggatcgaca ggcgaggagg tcgacggcat gcggacatat cattcgacta tctgcacact
     2101 tctggtcgcg gggctcgccg cgacgggcgg gtgttacaat ggatccggcg gcggccagac
     2161 cggcgacagc gcggcgaccg aggccggcgg cacgccgggt gacaccggcc agcagccgac
     2221 cggctccgat gccggtgaca ccgaccagcc gacgggcgag ccggtcgacg gcgatccgct
     2281 ggtctacagc ggcctgcgac gactgacggc cgacgagtac aacctgacgg tccgcgacct
     2341 gctcggcctc gacgcggcca gccaggcgct gctgcccaac gacccgctga gccccttcga
     2401 caacgactac cgcgaccaga ccgcctcgca ggcgctgatc gacggcgccg atctgctggc
     2461 ggccgacctc gcgaccgagg cgctcgtcga cccggtcgcc cgcaccacgc tggtcggctg
     2521 caccccggcc gggccggacg acgcggtgtg cttcgaccag ttcatcgcca gcttcggccg
     2581 tcgcgcgctg cggcgcaccc tcgagcccgc ggacatcgag cgcttctcgg tgttcctcga
     2641 ccactcgatc gaggccggcg acttctacgt cggcgtcgag tcggcgctgc gcgcatttct
     2701 ccagcacccc gagttcctgt atcgcgtcga gatcggcgag ccggtcgccg acgagccggg
     2761 tgtgtatcgc ctcggcagcc tcgagatcgc cgctcgcctg tcctacttcc tgtggggcag
     2821 cacgccgccc gactggctgc tcgatcaggc ggagggcgcc ggcctcgaca ccccggagaa
     2881 gatccgcgcc gccgcgcagc agctgctcgc tgacccgcgg gcgcgcgccc gcatcgcccg
     2941 cttccacgcg ctgtggatgg gctacatcac cctcccgcac ggggccgagc tggccgacgc
     3001 gatgaaggcc gagacgcagg cgctgatcga ccgcgtgctg ttcgacgaaa aaggcccgtg
     3061 gcaggacctg ctgcgcagcg aacagacctt cgtgtcgaac ttcctcgccg gccactacgg
     3121 cctgccgctg cccggcggcg acgggccgag ctgggtcgac tacggcgaca gcggccggcg
     3181 cggcctgctg tcgcacggca gcttcctctc ggtcggcgcg aagttcacgg acaccagccc
     3241 gacccagcgc ggcctgctga tccggacgcg tttgttctgt caggacatca agctgccgga
     3301 ggactccgac gtcgacgtcg acaacccgcc gggcgacccg aacgcctgca agccgcagcg
     3361 ctactcggag cacgcctcgg gcaagtgcgc cagctgtcac agcctcatgg acccgatcgg
     3421 ctttggcctc gagaactacg acatggccgg gcgctttcgc gagttcgagc ccgaccgccc
     3481 cgactgcccg atctccggcg agggcgagct gctcggcgtc ggcaagttca gcggcccggc
     3541 cgagctcggc gagctgatgc tcggcgtgcc cgacctcaat cattgtgtgc tcacccagct
     3601 gtatcgcttc gccatgggcc gctacgagct gcacgagcag gacacgctgg cgatccagcg
     3661 cctgctcaag ggcgtgcctg tgggcggcga cttgcccttc gaggcgacgc tgctcgagtt
     3721 catcgacacc cccgccttcc ggcaccgccg cgaggaaccg acggacatct gagg
//
LOCUS       DASWNJ010000089         2319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_354904, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000089 DASWNJ010000000
VERSION     DASWNJ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354904"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..>2319
                     /locus_tag="VGB85_01685"
     CDS             231..>2319
                     /locus_tag="VGB85_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0132750.1"
                     /translation="MNDDDGGTTDGEPLQSSQQRCLGGRYMLLDPIGAGGGAVVVRAR
                     DRQLPRKIVAVKLLRSRDEDLRRRFVQEAEVLADVAHPSIVQVLGRDSEGEEFYTVLE
                     LIDGLDLFEHLARSGPLAWRQVIDIGIQIAGALAVVHARGLVHRDVKPANIMLDASGR
                     VKLIDFGVVRITENYRAPTGTTQRRPTEIGVTLGTPGYLPLEAGLVVPNPSFDVFGLG
                     ATLYQLLTGKIPEEPILPVREARPGCDAPADLELVLTAALALEPEDRTQTAAELGAAL
                     AAVRAAHPERGTPSPRIDGRYELIGLAGTGAKADAKMAVHRGTGHDVVLQFLRADAPE
                     DVLRFAREAKLLSAFNHPALPRFYDYAPEASPPYIVMARAPGQPASRLCSPPRLKPAE
                     VAAVGVKLAQVLAVLHARGVLHRDINASNVMIDAHGAVTLLDFGCAELEEKFYDVPAG
                     ERRYLTPPEGRVSIPDGGIGRLGWSAPEVRHGTAWSDRSDVYSTGHLLFRLLTGKVPR
                     KGADPPTSPREFVITCPAEIASAIESTLRVDPQARPTAAQLAQRLLDALENEQTSGPR
                     VQADPPSLPVPDPDPATPPAQPAPVIVTAAKVPAVKLPRRTLAWAACFCVAVLVAWWA
                     QRPEHPVVAANSPTPPSLGRPPVSAASVSLGAASPAADMPADSATQRLADAGPALTEC
                     ARKAGRKVWVDLEV"
BASE COUNT          333 a          851 c          811 g          324 t
ORIGIN      
        1 tacggggcgg ccatcgtcac cgtcgacccc gacgcgctcg ccgtcgaatt cgtcgcggtc
       61 gccggcgtgc gcgacccgaa gtacggcggc gtccgctcgc gcaccggctt tcgtgtcgcc
      121 gcgggctcgt cccggatcac gcgggtgtcc gaggattagc ccccgagcag ccccatcttc
      181 agccttgact tgccccacca ccgtcgcctt atagacggcg aggggcgaag atgaacgacg
      241 acgacggcgg gaccacggac ggcgagccac tacagagcag ccagcagcgc tgcctcggcg
      301 gccgatacat gctgctggac cctatcggcg ccgggggcgg cgcggtggtc gtacgggcac
      361 gcgaccgtca gctccccagg aagatcgtcg ccgtcaagct gctgcgctcg cgtgacgagg
      421 acctgcggcg ccgcttcgtc caggaggccg aggtgctggc ggacgtcgcc catccctcga
      481 tcgtgcaggt gctggggcgc gacagcgagg gcgaggagtt ctacaccgtc ctcgagctga
      541 tcgacggtct ggacctcttc gagcacctcg cacgatcggg gccgctggcc tggcggcagg
      601 tgatcgacat cgggatccag atcgccggcg ccctcgctgt cgttcacgct cgtgggctcg
      661 tccaccggga tgtgaagccc gcgaatatca tgctcgacgc gagcgggcgc gtgaagctga
      721 tcgacttcgg cgtcgttcgc atcaccgaaa actacagagc cccgaccggc acgacgcagc
      781 ggcggccgac ggagatcggc gtgaccctcg gcacccccgg ctacctgccg ctcgaggcgg
      841 gcctcgtggt gcccaacccg agcttcgacg tgttcgggct cggggccacg ctgtatcagc
      901 tgctgaccgg caagatcccc gaggagccga tcctgccggt gcgcgaggca cgtcccggct
      961 gtgacgcgcc cgccgacctc gagctcgtat tgaccgccgc gctggcgctc gagcccgagg
     1021 accgcacgca gacggcggcc gagctcggtg ctgcgctcgc ggcggttcgc gccgcacatc
     1081 cggagcgcgg cacgccctcg ccgcgcatcg atggtcgcta cgagctgatc ggactcgcgg
     1141 gcaccggggc gaaggcggac gcgaagatgg cggtgcaccg cggcaccggc cacgacgtcg
     1201 tgctccagtt cctccgggcg gacgcgcccg aggacgtcct gcgcttcgcg cgggaggcga
     1261 agctgctcag cgccttcaac catccggcgc tgccacgatt ctacgactac gcgcccgagg
     1321 cgagcccgcc ttatatcgtc atggcccgcg cgcccggtca gcccgcatcg cggctgtgtt
     1381 cgccaccccg cctcaagccc gccgaggtgg ccgcggtcgg ggtgaagctc gcgcaggtgc
     1441 tggccgtgct gcacgcgcgc ggcgtgctgc accgcgacat caacgccagc aacgtgatga
     1501 tcgacgcgca cggagcggtg accttgctcg acttcggctg cgccgagctc gaagagaagt
     1561 tctacgacgt accggcgggc gaacggcgct atctgacgcc cccggagggc cgggtgtcga
     1621 tcccggacgg tgggatcggt cggctcggct ggtcggcgcc ggaggtccgg cacggcaccg
     1681 cgtggtccga ccgaagcgac gtctactcga cgggccacct gctgttccgc ctgctgaccg
     1741 gaaaggtgcc gaggaagggc gccgacccgc ccacgtcgcc gcgggagttt gtgatcacct
     1801 gccccgcgga gatcgcgtcg gcgatcgaga gcacgcttcg tgtcgaccct caagcacgcc
     1861 cgaccgcggc ccagctcgcc cagaggttgc tcgacgccct cgagaacgag cagacctccg
     1921 ggccgcgagt tcaggccgac ccgccgagcc ttccggtccc cgatccggac ccggcgacac
     1981 cgccggcaca accggcgccg gtgatcgtga ctgccgcgaa agtgccggcc gtcaagctgc
     2041 cgcgcaggac gctcgcgtgg gccgcgtgct tctgtgtggc agtgctcgtc gcctggtggg
     2101 cgcagcggcc cgaacacccc gtcgttgcgg cgaactcgcc gacccccccg agcctcggcc
     2161 ggccgccggt gtccgccgcg tccgtctccc tcggcgccgc gagcccggca gcggatatgc
     2221 cggccgacag cgccacccag cgactcgccg acgcgggtcc cgcgttgacc gagtgcgcgc
     2281 gaaaggccgg gcgcaaggtc tgggtcgacc tcgaagtgt
//
LOCUS       DASWNJ010000090         3059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_425851, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000090 DASWNJ010000000
VERSION     DASWNJ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425851"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1606
                     /locus_tag="VGB85_01690"
     CDS             <1..1606
                     /locus_tag="VGB85_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132751.1"
                     /translation="LDWHPLVLALGPVPLRLATWVVLWWERLFPRVLVALWLREAQQC
                     DAQALSARARRLARACWAGLAVALALAAAVLGYMSEETGGRLEGAICLALAAASVALG
                     VLLGARALPRAYQAAGPVVALWLGVGLTFHAVNFMLLNVGAFALATLTAYLLCGAGAP
                     AVRGLQRICHALARRGVPVPEHMSRETAIACEDPDLPQLQRDAAALPGWAIAGAGGLL
                     LLGAAIALAHGPKAGLHWWHAAWLSAGTGLLWLGWRRARSHGDADLPLRYPWAHGPAG
                     RLVAGGLLTYHLVALLAWQLPRWPALPYRDDARALVDPWMELSFTRQLWAMFAPDPPR
                     QNLTLRTRIVDARGTSHDLRSELEHPEHLVRPYLWSDRARKVIEGVAGRRPGLTAWHA
                     RHLCRRWAIEHDGELPARVILERVTAPIVPDDPRPALDAFWADARSEPLVSIDCAEAP
                     FAQPAPEVRARHGLPPAPPGSLRYTWPKGQPETWAERRAAIGPLPLLWPLLALVLAGA
                     AAVWAREDRRRHVRRVAARRREGAKA"
     gene            1743..>3059
                     /locus_tag="VGB85_01695"
     CDS             1743..>3059
                     /locus_tag="VGB85_01695"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0132752.1"
                     /translation="MTLADQRRRLPIVDALPRGGTRWSNPETRGPKKGPAWAIWELTL
                     ACDQKCMHCGPRAGQRRPHELDTEECLRVVAELAELGCGEVVLIGGEAYLRNDFILII
                     RAIRAAGMSCTMTTGGLNLNDERVEAMIEAGVSGVTVSIDGLEATHDAIRGVPGSWQR
                     AFAALRRVKAAGGKIACNTQINRRTRGELAPLLELIAAEGIHSWQLQITVPHGNAADH
                     PEMILQPYDFIELFTELDPLIDRCEALGVRMWPANNLGYFGPLERRLRKSLRTHWRGC
                     TAGVTTLGLDSDGGVKNCPSLGGELNVGGSWREHGLRKLWEESYQLGYIRTRTRDDLW
                     GYCRDCYYADVCMAGCTAASEPLLGRPGNNPFCHHRAMMLDREGLRERIEQVRAAPGI
                     SFDNGLFRVIREHKDPALRELHGPVQIDEPRVSRAVEPNGPGYPIDL"
BASE COUNT          386 a         1156 c         1101 g          416 t
ORIGIN      
        1 cctcgactgg cacccgctgg tcctcgcgct cggcccggtc ccgctgcgcc tcgccacgtg
       61 ggtcgtgctg tggtgggagc ggctgttccc gcgggtgctc gtcgccctgt ggctgcgcga
      121 ggcgcaacag tgcgatgccc aggccctgtc cgcgcgcgcg cgccggctcg cgcgcgcctg
      181 ctgggccggc ctcgccgtcg cgctcgcgct cgccgcggct gtcctcggat acatgtccga
      241 agagacaggc ggtcgcctcg agggcgccat ctgcctggcg ctcgcggccg ccagcgtcgc
      301 gctcggcgtg ctgctcggcg cgcgggcgct gcctcgcgcg tatcaggccg ccggacccgt
      361 cgtcgcgctg tggctcggcg tcggcctcac gttccacgcc gtcaacttca tgctgctcaa
      421 cgtcggcgcg ttcgcgctgg cgaccctgac cgcctacttg ctttgcgggg cgggagcgcc
      481 ggccgtccgc ggcctccagc ggatctgcca cgcgctcgcg cgccgcggcg tacctgtccc
      541 cgagcacatg tcccgcgaga cagcgatcgc ctgcgaggac ccggacctgc cccagctgca
      601 gcgcgacgcc gccgcgctgc ccggctgggc gatcgccggc gccggtggcc tgctgctgct
      661 cggcgccgcg atcgcgctcg cccacggacc gaaggccggc ctgcactggt ggcacgccgc
      721 ctggctgtcc gcggggacag gtctgctgtg gctcggctgg cgtcgcgccc gcagccatgg
      781 cgacgccgac ctgccgctgc gctacccgtg ggcccacggc ccggcggggc ggctcgtcgc
      841 tggcggcctg ctcacctacc acctcgtcgc cctgctggcc tggcagctgc cgcgctggcc
      901 ggcgctgccg taccgcgacg acgcccgcgc gctcgtcgac ccgtggatgg agctgagctt
      961 cacgcgccag ctgtgggcga tgttcgcccc cgacccgccg cggcagaacc tgaccctgcg
     1021 cacgcggatc gtcgacgcgc gtggcacatc ccacgacctg cgcagcgagc tcgagcaccc
     1081 cgagcacctg gtccgcccgt acctgtggtc ggatcgcgcg cgcaaggtga tcgagggggt
     1141 cgccggccgc cgccccggcc tcaccgcgtg gcacgcccga cacctgtgcc gtcgctgggc
     1201 gatcgagcac gacggcgagc tgccggcccg cgtgatcctc gagcgcgtga cagcgccgat
     1261 cgtcccggac gacccccgcc ccgcgctcga cgccttctgg gccgacgcac gcagcgagcc
     1321 gctggtcagc atcgactgcg ccgaggcccc gttcgcccag ccggcccccg aggtgcgcgc
     1381 gcgccacggc ctgcccccgg cccccccggg gtcgctgcgc tacacctggc ccaagggaca
     1441 gcccgagacc tgggccgagc gccgggccgc gatcggcccg ctgccgctgc tgtggccgct
     1501 gctcgccctg gtgctcgccg gcgcggccgc ggtgtgggcc cgcgaggacc gccgccgcca
     1561 cgtccgccgc gtcgccgcgc gaaggcgcga gggcgcgaag gcgtgacggc gagtgacggc
     1621 gagtgaccgc gcgtgatcga tcgtgatcga ccgacaatga actcgtcgag gcgtcgcgcg
     1681 gccggtcgca aaatgcgaca tcatggcgcc atgattaatc ctgcaccgac acggggtcgc
     1741 cgatgacgct cgcggaccag cggcggcgac tgccgatcgt cgacgccttg ccccgtggcg
     1801 gcacgcggtg gagcaacccg gagacgcgcg gccccaagaa gggcccggcc tgggcgatct
     1861 gggagctgac gctcgcgtgt gatcagaagt gcatgcactg cgggccgcgt gccggccagc
     1921 gccggcctca cgagctcgac accgaggagt gcctgcgcgt cgtcgcggag ctggccgagc
     1981 tcggctgcgg ggaggtcgtg ctgatcggcg gcgaggcgta cctgcgcaac gacttcatcc
     2041 tgatcatccg ggcgatccgg gcggccggca tgagctgcac gatgaccacc ggcgggctca
     2101 acctgaacga cgagcgcgtc gaggcgatga tcgaggccgg ggtgtccggc gtgacggtct
     2161 cgatcgacgg cctcgaggcg acccacgacg ccattcgcgg cgtgcccggg agctggcagc
     2221 gcgccttcgc ggcgctgcgg cgggtgaagg cggccggggg aaagatcgcc tgcaacacgc
     2281 agatcaaccg ccgcacccgc ggcgagctag cgccgctgct cgagctgatc gcggccgagg
     2341 gcattcactc gtggcagctg cagatcaccg tgccgcacgg caacgcggcc gaccacccgg
     2401 agatgatcct ccagccgtac gatttcatcg agctgttcac cgagctcgac ccgttgatag
     2461 atcgctgcga ggcgctcggc gtgcgcatgt ggcccgccaa caacctcggc tacttcggcc
     2521 cgctcgagcg ccggcttcgc aagtccctgc gcacgcactg gcgcgggtgc acggcgggcg
     2581 tcacgacgct cggcctcgac agcgacggcg gggtcaagaa ctgcccgagc ctcggcggtg
     2641 agctcaacgt cggcggcagc tggcgcgagc acggcctgcg caagctgtgg gaggagagct
     2701 accagctcgg gtacatccgg acccgcacgc gcgacgacct gtggggctac tgccgcgact
     2761 gttactacgc cgacgtctgt atggccggct gcaccgcggc gagcgagccg ctgctcgggc
     2821 gccccggcaa caacccgttt tgtcaccacc gggcgatgat gctcgaccgc gagggcctgc
     2881 gcgagcgcat cgagcaggtc cgcgccgcgc ccggcatatc gttcgacaac ggcctgtttc
     2941 gcgtgatccg cgagcacaag gacccggccc tgcgcgagtt gcatgggccg gtgcagatcg
     3001 acgagccgcg ggtgtcgcgg gcggtcgagc ccaatggtcc agggtacccg atcgacctg
//
LOCUS       DASWNJ010000091         2211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_178187, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000091 DASWNJ010000000
VERSION     DASWNJ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178187"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VGB85_01700"
     CDS             <1..373
                     /locus_tag="VGB85_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0132753.1"
                     /translation="AKVGVLERKDYYPAHLSGGQQQRVAIARALAMEPAVMLFDEPTS
                     ALDPELVGEVLRVMRDLAAEGRTMLVVTHEMGFARDVASEVVFLHQGRLEAKGPPSTM
                     FGSNTPSERFRQFLAGSRREE"
     gene            457..1530
                     /locus_tag="VGB85_01705"
     CDS             457..1530
                     /locus_tag="VGB85_01705"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0132754.1"
                     /translation="MPPKLALALTFAGALALAQPAAAAEVGGPATLFKPAVAPKSAAP
                     KKAAPSKVAAPGTGKPASAKRKEAVARLQTPATAAALALAQQGPELRVGVEGSYPPFS
                     EVGVDGQLKGFDIDVANALCEEMRARCRFERQDWGRIQEGLLARETDFVVASLSISEE
                     RKQRYAFTNRYYHVPGKFVARQGGEPLPAGFDEVGLRGARIGVQQGTAHAAYLAARFG
                     ETVRTTRYPSVDAALDALGRGEVTLVLADALVIDQNAMRSEAGRDLRFVGPDLLYPDW
                     FGEGAGIAVRKEDAGLRERLDGALARLHADGRYRAIAGRYFGFDISGTAAPPREERRV
                     AGVPPRPQRELPQLIDGPNTRDR"
     gene            1668..>2211
                     /locus_tag="VGB85_01710"
     CDS             1668..>2211
                     /locus_tag="VGB85_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0132755.1"
                     /translation="MATLAAGIADAKEWTKVRFGVEGAYPPFSETTPSGELKGFDIDI
                     ANALCKQMKAECQLVQQDWDGIIPALLARKYDAIIASMSVTEERKQKVDFSKKYYQTP
                     ARFVRKKDSGIEINADSLKGKKVGVQRATIHDSFLTDNYGGQVEIVRYGTQDEANLDM
                     VAGRVDLLLADTVALSEGFLK"
BASE COUNT          350 a          744 c          821 g          296 t
ORIGIN      
        1 ggcgaaggtc ggggtgttgg agcgcaagga ctactacccg gcgcacctct cgggtgggca
       61 gcagcagcgg gtggcgatcg ccagggcgct ggcgatggag ccggcggtga tgctgttcga
      121 cgagccgacc tcggcgctgg acccggagct ggtgggcgag gtgctccgcg tgatgcgcga
      181 cctcgcggcc gaggggcgga cgatgctcgt ggtgacgcac gagatgggct tcgcgcggga
      241 cgtggcgagc gaggtcgtgt tcctgcacca gggccgcctc gaggcgaagg gtccgccgtc
      301 gaccatgttc ggcagcaaca cgccgtcgga gcggttccgg cagttcctcg ccggcagccg
      361 gcgggaggag tagcgaacgc tgcggtagaa ccggcttgac gtcgttcccc tcggcccgca
      421 ataggccgtc gccacgcgcg tgctcccgag gaacccatgc cgccgaagct cgccctcgcc
      481 ctgaccttcg ccggggcgct cgccctcgcc cagcccgccg cggccgcgga ggtcggtggc
      541 cctgcgacat tgttcaagcc ggcggtggca cctaagtcgg cggccccgaa gaaggcggcc
      601 cccagcaagg ttgccgcgcc ggggacgggg aagccggcga gcgcgaagcg caaggaggca
      661 gtcgcgcgcc tgcaaacgcc ggccacggct gccgcgctcg cgctggcgca gcaagggccg
      721 gagctgcggg tcggcgtgga gggctcctac ccgccgttca gcgaggtcgg ggtggacggc
      781 cagctcaaag gcttcgacat cgacgtcgcg aacgcgctct gcgaggagat gagggcccga
      841 tgccgcttcg agcggcagga ctggggccga atccaggagg ggctgctggc ccgcgagacc
      901 gacttcgtcg tggcctcgct cagcatcagc gaggaacgca agcagcgcta cgccttcacc
      961 aaccgttact accatgtgcc cggcaagttc gtggcgcggc aggggggcga gccgctgccg
     1021 gcaggtttcg acgaggtcgg tctgcgaggg gcgaggatcg gcgtccagca gggcacggcg
     1081 cacgccgcct acctggcggc ccgcttcggc gagaccgtgc ggacgacacg ttaccccagt
     1141 gtcgacgcgg cgctcgacgc gctcggccgc ggcgaggtga cgctcgtgct cgccgacgcg
     1201 ctggtgatcg accagaacgc catgcgctcc gaggcggggc gggacctgcg cttcgtcggc
     1261 ccggacctgc tctacccgga ctggttcggc gagggggcgg ggatcgcggt gcgcaaggag
     1321 gacgcgggcc tgcgcgagcg gctcgacggc gcgctggcgc ggctccacgc cgacgggcgc
     1381 taccgggcga tcgccggccg ctacttcggc ttcgacatct ccggcaccgc agcaccgccg
     1441 cgtgaggagc gccgcgtcgc cggggtgccg ccccgcccgc agcgggagct gccgcagctg
     1501 atcgacggcc cgaacacgcg cgaccggtag agcacttccg ggccgactcc gcctgccgcc
     1561 tcgcttgacg ggcggcggcg cgtcgtgaac agtctcggcc cagggagccc ttgggaggga
     1621 tcaggatgcg gaaacgactg ctcgcggcca cggtcgccgg gttcgtgatg gcgacgctgg
     1681 cggccgggat cgccgacgcg aaggagtgga ccaaggtccg gttcggggtc gagggcgcct
     1741 acccgccgtt cagcgagacg acccccagcg gcgagctgaa gggtttcgac atcgacatcg
     1801 cgaacgcgct ctgcaagcag atgaaggccg agtgccagct ggtccagcag gactgggacg
     1861 gcatcatccc ggcgctgctc gcccgcaagt acgacgcgat catcgcgtcc atgtcggtga
     1921 ctgaggagcg caagcagaag gtcgacttca gcaaaaaata ttaccagacc ccggcccgtt
     1981 tcgtccgtaa gaaagactcg ggtatcgaga tcaatgccga ctcgctcaag ggcaagaagg
     2041 tcggcgtgca acgcgccacc atccatgaca gcttcctgac cgacaactac ggcggccagg
     2101 tcgagatcgt ccgctacggc acccaggacg aggcgaacct cgatatggtc gccggccggg
     2161 tcgatctgct gctcgccgac accgtggccc tctcggaagg cttcctcaag t
//
LOCUS       DASWNJ010000092         3117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_143135, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000092 DASWNJ010000000
VERSION     DASWNJ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143135"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VGB85_01715"
     CDS             complement(<1..520)
                     /locus_tag="VGB85_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132756.1"
                     /translation="MNGRKHNFTHFFSALGIAFSTLAVGATVACDAMDGTDERVYSGG
                     SVELSVGEVSSISGGDEGDTNGGEGCTLTQGYWKNHSDYAENASQHKAWPISEDTQLC
                     GQSWYDILHTAGKGDAWYVVAHQYIAASLNVASGAAVTAEVTAALAAAEGFLADCKIS
                     KAEKADAIASKDV"
     gene            815..1426
                     /locus_tag="VGB85_01720"
     CDS             815..1426
                     /locus_tag="VGB85_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132757.1"
                     /translation="MSELYSVHIEAVHEGEVTFRVTTIHPDAGPPPEDATFPMNLLID
                     LWGLLDRGFVSLIKGCKLGAEQAIALARDPAYATRMAALRGLACGREEQCSAADIRDL
                     ERQMTVGEPPRLRGLEVQGWGGAGKQQHVFLPADPGGFAGAIAPLVDCCAVDEHENDG
                     AYDDWPRGKDAPLPRVRIWLALRDPALLGFVRAGWRFDSASYY"
     gene            complement(1444..2103)
                     /locus_tag="VGB85_01725"
     CDS             complement(1444..2103)
                     /locus_tag="VGB85_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132758.1"
                     /translation="MKQVVAGVLRGRSVWQLVAIAAVCTSLALYIIYSSYSSTLRQLK
                     PVLIVLFGIAATALILAVLRCSEDIERHGGAGKRWRRLVLAVFGLAAAISFVTYSFGH
                     YETDMHAACNGSLLPDTLPARRAELAEAEQRLRSPFALLPLLLDDEAERECARSRADL
                     ARVEQGLCTNWPMLDVSCACGEERFPYTRCKEPRCLYTAGKPDRFYCVADPIPEGYDN
                     F"
     gene            complement(2290..>3117)
                     /locus_tag="VGB85_01730"
     CDS             complement(2290..>3117)
                     /locus_tag="VGB85_01730"
                     /inference="COORDINATES: protein motif:HMM:NF044477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132759.1"
                     /translation="RQHGWSDRGITLAGLRQLKKAMIAAGSVAKLAAMPGLQADRAGV
                     LPGGVAILLSVFEGLGIEQMATTGGSMREGLLYDLLGRIRHEDARDLTIRQFCGRYHV
                     DMDQAARVERLALHCLDQVASSWGIDPVIGHQMLGWAARLHEIGLSIAYSGYHRHGAY
                     LVANSDMPGFSRADQDLLATMLLGQRKKLTREVFAGLPPQEVELALRLCVLLRLAVRL
                     ARSRSPRPLPTFTVTAKKNSLEIGFPADWIEGHPLTLADLNEEGSKLQGVGVELRVR"
BASE COUNT          490 a         1057 c         1071 g          499 t
ORIGIN      
        1 gcacgtcctt gctggcgatc gcgtccgcct tctcggcctt ggagatcttg cagtcggcga
       61 ggaagccctc ggcggcggcc agcgcggcgg tcacctccgc ggtcaccgcg gcgcccgacg
      121 cgacgttgag gctcgcggcg atgtactgat gcgccacgac gtaccaggcg tcgcccttgc
      181 cggccgtgtg caggatgtcg taccagctct gcccgcacag ctgggtgtcc tcgctgatcg
      241 gccacgcctt gtgctgcgac gcgttctcgg cgtagtcgct gtggttcttc cagtagccct
      301 gggtcaacgt gcagccctcg ccgccgttgg tgtcaccctc gtcaccaccc gagatcgagg
      361 agacctcccc gaccgacagc tcgaccgagc caccggagta gacgcgctcg tccgtgccgt
      421 ccatcgcgtc acaggcgaca gtggcgccga ccgcgagggt cgagaacgca atgccgagcg
      481 ccgagaagaa gtgggtgaag ttgtgcttgc gtccgttcat gcccgttcga catggaccct
      541 gtatatattt ccaaatgctc gatcgacatt ttttatcatc acattcatag tatgtgcata
      601 gcgaagatac gtgacaaatg aaagccggcg gacccgcgat accccacatt gaccgtgcag
      661 cagtggcagg atccggctac ccggctaccg cgcgatgtcc gccgcagcgc gcgtaaaccc
      721 tgttctctgc cgatctcccc cgtttcgtag cacagtgact tacatcgtgg acgtgccacc
      781 gacccggacg cgcatggttt atcgtggcgg ggtcatgagt gagctgtaca gcgtccacat
      841 cgaggcggtg cacgagggcg aggtcacgtt tcgcgtgacg acgattcacc ccgacgccgg
      901 accgccgccc gaggacgcga ccttccccat gaacctgctg atcgatctat ggggtttgct
      961 ggatcgcggg ttcgtctcgt tgatcaaggg ctgcaagctc ggcgcggagc aggcgatcgc
     1021 gctcgcgcgc gacccggcgt acgcgacacg catggccgcg ctgcgcggcc tggcctgcgg
     1081 gcgcgaggag cagtgcagcg cggcggacat ccgggacctc gagcggcaga tgacggtcgg
     1141 cgagccgccc cgcctgcgcg gcctggaggt ccagggctgg gggggagccg gcaagcagca
     1201 gcacgtgttc cttccggccg acccgggcgg cttcgccggg gcgatcgcac cgctggtgga
     1261 ctgctgtgcg gtcgacgagc acgagaacga cggcgcatac gacgactggc cgcgtggcaa
     1321 ggacgcaccc ctgccgcgcg tgcgcatctg gctcgcgctc agggacccgg cgctgctcgg
     1381 cttcgtgcgc gccggctggc gcttcgactc ggccagctac tattgagccg cggcctggcg
     1441 atatcagaaa ttatcgtagc cctcggggat cgggtcggcg acgcaataaa agcggtccgg
     1501 cttgcccgcg gtgtacaggc agcgcggctc cttgcagcgg gtataaggga agcgctcctc
     1561 gccgcaggcg cagctgacgt cgagcatcgg ccagttggtg cacagcccct gctcgacgcg
     1621 cgcgaggtcg gcccggctgc gggcgcactc gcgctcggcc tcgtcgtcga gcagcagggg
     1681 cagcagcgcg aacgggctgc gcaggcgctg ctcggcctcg gcgagctcgg cgcggcgggc
     1741 cggcaaggtg tccggcagca gcgagccgtt gcaggcggcg tgcatgtccg tctcataatg
     1801 cccgaagctg tacgtcacga acgagatcgc agcggcgagc ccgaagaccg cgagcacgag
     1861 gcgtcgccac cgcttcccgg cgccgccgtg gcgctcgatg tcctcgctgc agcggagcac
     1921 ggccaggatc agcgccgtgg cggcgatccc gaacagcacg atgagcaccg gcttgagctg
     1981 cctcaacgtc gagctatatg aactatagat aatgtacagc gcgaggctcg tgcacaccgc
     2041 cgcgatcgcg acgagctgcc agacgcttcg accacgcagg accccggcga ccacctgctt
     2101 catcgcctcc atcttaccag cgttcgcgga cgccacaccg ggcgcgtctg cgcacgcgtc
     2161 gtaccgccac gtgcgaggcc tgcgtggacc tcggaccgac accgcctgca tctcctggta
     2221 catgccaggc gaggcgcccc ccgagtacga gcacgtcggc atctgcggcc agccgtgatc
     2281 ggccccgcgc tatcgcacgc gcagctcgac gccgacgccc tgcaatttgc tgccctcctc
     2341 gttgaggtcg gcgagggtca gcgggtggcc ttcgatccag tcggcgggga agccgatctc
     2401 gaggctgttc ttcttggccg tgacggtaaa ggtcggcagc ggacgcgggc tgcggctgcg
     2461 ggccaggcgg acggcgaggc gcagcagcac gcacaggcgc agcgccagct cgacctcttg
     2521 cggcggcagg ccggcgaaca cctcgcgggt gagcttcttg cgctggccga gcagcatggt
     2581 cgccagcagg tcctggtcgg cgcgcgagaa cccgggcata tcggagttgg cgaccaggta
     2641 ggcgccgtgg cgatggtatc cggagtacgc gatcgacagc ccgatctcgt gcaggcgggc
     2701 ggcccagccg agcatctgat ggccgatgac ggggtcgatg ccccagctgc tcgcgacctg
     2761 gtcgaggcag tgcagcgcga ggcgttcgac ccgcgcggcc tggtccatgt cgacgtgata
     2821 gcgcccgcaa aattgccgga tcgtcaggtc gcgcgcgtcc tcgtggcgga tgcggccgag
     2881 caggtcatat aataaaccct cgcgcatcga gccgccggtg gtcgccattt gttcgatgcc
     2941 gaggccctcg aacacggaca gcaagatcgc cacaccgccg ggcagcacgc cggcgcggtc
     3001 cgcctgtagt cctggcatcg cggcgagctt ggcgacgctg ccggcggcga tcatcgcctt
     3061 cttgagctgg cgcaggcccg cgagcgtgat gccgcggtcg ctccatccgt gttgccg
//
LOCUS       DASWNJ010000093         1765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_425859, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000093 DASWNJ010000000
VERSION     DASWNJ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425859"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VGB85_01735"
     CDS             <1..501
                     /locus_tag="VGB85_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132760.1"
                     /translation="VPAEGNAFCMFSGIGDHGLRSREEATAAWKEGIAHFISAMSWND
                     HTQADGKFKYYKNQAPHTFETVDLDAAGSFAINWHATQCSPTIAGRAVEQDWLRHYWN
                     YLTDAGLPTPTVSEIRAQVNNLYSNGGWTATNVYSKLDAALVTAGQVANQSRWANTAA
                     THGANF"
     gene            739..>1765
                     /locus_tag="VGB85_01740"
     CDS             739..>1765
                     /locus_tag="VGB85_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132761.1"
                     /translation="MSACGGDPAATGGDASTGLVDPPSATTTPTDGAGTEGSRTTGDG
                     GSGTTGTSPATGDGTSGTDGASTTSTTNSITTPGTGSGPGSSSATDTEASSSSATSTG
                     DTDGLDGACGPAPRNDPTSWSVGLGAPGEYMGVATIGARSGAVVYTTNFDAAIDLGGG
                     PIDPAGASHVLVASHAPSGALQWADHVGGPPQTTNILDFDAATDCAGNVVLAGTFSGE
                     ITVQRTTLTAVPGVEFDDGMSFPTVDWFVMKFGPDGALLWARRFGDLRSQRLRGVGVQ
                     VDGAIVIAGDVQGTMDLGGPPVVTAGPYPDTVLAALDPGGAFLWQRHVAGTQGAQFMR
                     VDVGPDDQ"
BASE COUNT          277 a          605 c          624 g          259 t
ORIGIN      
        1 gtacccgccg agggaaatgc gttttgtatg ttctccggta ttggggacca tggcttgcgt
       61 tcacgcgagg aggccaccgc ggcgtggaag gaaggtatcg ctcatttcat ttcggccatg
      121 tcgtggaacg accacacaca ggctgacgga aaattcaagt actacaagaa tcaggcgccg
      181 cacacttttg agacggtcga tctcgacgcg gccggatctt ttgccatcaa ctggcacgcg
      241 acccaatgct cgccgacgat cgcgggcagg gcggtcgagc aggactggct gcggcactac
      301 tggaactacc tcacggatgc cggactgccc acgccgacag tctccgagat cagggcacag
      361 gtcaacaatc tctattcgaa cggtggttgg acggccacca acgtctacag caagctcgat
      421 gcagctctcg tcaccgcggg ccaggtcgcc aaccagtcgc gctgggcgaa cacggccgcg
      481 acccacggcg cgaacttctg agctccgttg gcatttccgg gcccagacgg gctggctcgg
      541 ccttcctcaa gcgccccggg aggattactc ccgcggcccc ccgagtcgcg gtggtatctg
      601 cggatccgtg ccgcacgcgc gccgtgctcg acgtacggtg gatcactcgc ggggaccggg
      661 ctggcgctcg ccgcggtcaa cgcttaccct catacaatgc acaccgctcg gtaccacttg
      721 atcatgctct cggcgggctt gtctgcgtgc gggggcgatc cggcggcgac cggcggcgac
      781 gcctcgaccg ggctggtgga tccgccgagc gccacgacga ccccgaccga cggcgccggc
      841 acggagggct cgcgaaccac gggcgacggg gggtctggca ccaccgggac ctcgccggcc
      901 acgggagatg ggacgtccgg gaccgacggc gccagcacga cttcgaccac gaactcgatc
      961 accacccccg gtacgggctc ggggcccgga tcgtcgagcg cgacggacac ggaggcatcg
     1021 agctcgtccg ccacctcgac gggcgatacg gacggcctcg acggggcgtg cggcccggcc
     1081 cccaggaacg acccgacgtc gtggagcgtg ggcctcggcg cgccggggga gtacatgggg
     1141 gtggcgacga tcggcgcgcg ctcgggggcc gtcgtgtaca cgaccaactt cgacgcggcc
     1201 atcgacctcg gcggcggccc catcgacccg gccggggcca gccacgtgct cgtggccagc
     1261 cacgcgccgt cgggggcgtt gcagtgggcc gatcacgtcg ggggcccgcc ccagacgacc
     1321 aacatcctcg acttcgacgc cgcgacggac tgtgcgggca acgtcgtgct cgccgggacg
     1381 ttcagcgggg agatcacggt ccagcggacg acgctgacgg cggtgcccgg ggtcgagttc
     1441 gacgacggca tgtcgttccc gacggtcgac tggttcgtga tgaagttcgg tcccgacggc
     1501 gcgctgctgt gggcgcggcg cttcggtgac ctgcgctcgc agcggctgcg cggcgtcggt
     1561 gtgcaggtcg acggcgcgat cgtcatcgcc ggcgacgtcc agggcacgat ggatctcggc
     1621 ggtccgccgg tcgtcacggc cggcccatac ccggacacgg tgctcgccgc gctcgatccg
     1681 ggcggggcct tcttgtggca gcggcacgtc gcggggacgc agggcgccca attcatgcgc
     1741 gtcgacgtgg gccccgacga ccaga
//
LOCUS       DASWNJ010000094         5067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_213372, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000094 DASWNJ010000000
VERSION     DASWNJ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213372"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VGB85_01745"
     CDS             <1..437
                     /locus_tag="VGB85_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132762.1"
                     /translation="APPITKTAIEPVDGNPAGGGTKVIAGEDPTEARYEMRVEPPADA
                     VAGREGTVKVTIVPKAPWHMNLDFPTSLALSPPDGVTLAKAELKKADARKLDENSAEF
                     DVKFTPAAAGDKQFTGKFKFAVCQDEACSPVTEEVAFKVAVK"
     gene            482..2899
                     /locus_tag="VGB85_01750"
     CDS             482..2899
                     /locus_tag="VGB85_01750"
                     /inference="COORDINATES: protein motif:HMM:NF018504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132763.1"
                     /translation="MQEASSRSRIDALAAAVAQDFESTRRVLSFEEWFELLCEAPQVH
                     ARNAAQYLRDCFDFYGRRDVRIPSGKVARFRLFDCDFDDHGPGPNNARLVGQERAQNA
                     FYEAIDSFVRIGRVNKLLLLHGPNGSAKTTFLDSVMRAMEAYSQTDVGALYRFSWVFP
                     NKAARSGGIGFGTGPFGGAISEETYAKLSAEAVDSKLADENKDCPVFLLPLAMREKLL
                     ADVLDKARAREHAAGGTAGPGDSSQRSLQEFAYSEAILRGDLNQRNRRIFDALLGGYQ
                     GDLRKVYQHVQVERLFLSRQYRSGLVTVEPKQTVDARSYPVTGERAFASLPANVGGQV
                     LYACQGDLVDANRGLVNFSDLLKRPYEHYKYLLTATESGVVALDHVVLALDLVFTGSA
                     NDLNLLEFRALRSGEYQSLRGRLELIPVPYLLDYRVERKVYQEQVGDVLRGIHIAPHV
                     PRILALWGVMTRLRAPDPAAYSAKLQPVLARLTPLDKADLYAYGRVPAGLTSDEAREL
                     LAAVPKMHEERFQQAVVKAESGDPILLGDYEGSFGASVRDLKRVLLAAASDPGTNAVT
                     VPRLFKELRRFMADSVNFRWMLLTPKAPGPDGSRSDGGFHALEGEGSITQAVWNRWLD
                     LSDWEVREAMGLVDESRYLDLFKKYVKHVSHHLKRERMFDAVTGDLRNPDEKFMQGLE
                     KTMDPKAGPNFRADLLSRIGAWALSHPDEEPAYAEIFADYFGRLREDYYRQQKAVVGR
                     GIQRMLELLADDRRSENPLAPQEEQVARHAVDVLLGNCDDKSVRRERHSRETLRDTLV
                     QLSKFRY"
     gene            complement(2976..3530)
                     /locus_tag="VGB85_01755"
     CDS             complement(2976..3530)
                     /locus_tag="VGB85_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132764.1"
                     /translation="MFKPFLLPLLCSFLSLLPACQNSEDIEARSAELTANPGLRVTIS
                     NAGELPPETVHAIAKQIEGNGEGNGAATVRVKKEDDASPVLEIELWGGAMPDAGTVSD
                     KLKTTFPALASATIVTATIPAGQPPPLPIVAVDDDLAPADAEQQIRDQLAADGVDGTV
                     NVKVEDGPEGRRVEINVKKTETTP"
     gene            3672..4583
                     /locus_tag="VGB85_01760"
     CDS             3672..4583
                     /locus_tag="VGB85_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132765.1"
                     /translation="MLARWLCVALVGSACTRNEPEVVAVAPVAEAPAPAREPPPRAAS
                     GVELAALDALLRPCQGEEEGHPLGTLVSVERLGAWKDRPVYLARTTGGGDVRMLLFAD
                     TTGSCPAFVLGPEAAQVRGDFFGEGTQRTAGLLSQASDGVRCDNDACPVAVLLRDEDG
                     VVLAAANPGLECETYTLASVRLFADRDSIELRCSGGTVDTAREIQLLHAFGRELRPLL
                     AFDAGITTVTLSEDETRECTSRSRDGSYEIKATGAAPVVEVFAVSDLQAATRATWTFD
                     AAQQRMVQGTPVPVKLPAPRATCRAAG"
     gene            complement(4580..>5067)
                     /locus_tag="VGB85_01765"
     CDS             complement(4580..>5067)
                     /locus_tag="VGB85_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEY0132766.1"
                     /translation="DLGCWPGSWMQYAAQKVGDTGLVVGIDLRPVTVALPANVHHAVA
                     DVWEWSPAVLASEGQEVRFDLVVSDMAPQTTGDKHTDQFRSEALATRALEIARQALRP
                     GGHLVAKVFQGGQFPELLKQWRASFQETKPYHAKNTRSGSTEQYLVGRGLRAAAVLPQ
                     T"
BASE COUNT          759 a         1710 c         1857 g          741 t
ORIGIN      
        1 ccgcgccgcc gatcaccaag accgcgatcg agccggtcga cggcaacccg gccggcggcg
       61 ggaccaaggt gatcgccggt gaggatccga ccgaggcgcg ctacgagatg cgcgtcgagc
      121 cgccggccga cgccgtcgcc ggccgcgagg gcaccgtcaa ggtgacgatc gtgcccaagg
      181 cgccgtggca catgaacctc gacttcccga cctcgctggc gctgagcccc ccggacggcg
      241 tgacgctggc caaggcggag ctcaagaagg ccgacgcgcg caagctcgac gagaacagcg
      301 ccgaattcga cgtcaagttc acccccgcgg ccgccggcga caagcagttc accggcaagt
      361 tcaagttcgc cgtgtgccag gacgaggcct gctcgccggt cacggaagag gtcgcgttca
      421 aggtcgccgt caagtaggtc gcatcttcag cgtcctcatc aggggcaggg ggtcgggagc
      481 catgcaggaa gccagcagca ggagccgcat cgacgcgctg gccgcagccg tcgcccagga
      541 cttcgagtcg acgcgccgcg tcctcagctt cgaggagtgg ttcgagctgt tgtgcgaggc
      601 gccgcaggtg cacgcccgca acgcggccca gtacctgcgc gattgcttcg acttctacgg
      661 ccgccgcgac gtgcggatcc ccagcggcaa ggtcgcccgc tttcgcctgt tcgactgcga
      721 cttcgacgac cacggcccgg gcccgaacaa cgcgaggctg gtcggccagg agcgggcgca
      781 gaacgccttc tacgaggcga tcgacagctt tgtccgcatc ggtcgcgtca acaagctgtt
      841 gctgctgcac gggcccaacg gctcggcgaa gacgaccttc ctcgacagcg tgatgcgggc
      901 gatggaggcc tactcgcaga ccgacgtcgg cgcgctgtac cgcttctcgt gggtgttccc
      961 caacaaggcg gcgcgctcgg gcgggatcgg cttcggcacc gggcctttcg gcggtgcgat
     1021 cagcgaggag acctacgcca agctcagcgc cgaggcggtc gacagcaagc tcgccgacga
     1081 gaacaaggac tgcccggtgt tcctcctgcc gctggcgatg cgcgagaagc tgctcgccga
     1141 cgtgctcgac aaggcccgcg cgcgcgagca cgcggccggc ggcaccgcgg gtcccggcga
     1201 cagctcgcag cgctcgctgc aggagttcgc gtacagcgag gcgatcctgc gcggcgacct
     1261 caaccaacgc aaccggcgca tcttcgacgc gctgctcggc ggatatcagg gcgacctgcg
     1321 caaggtgtac cagcacgtgc aggtcgagcg cctgttcctc agccggcagt atcgcagcgg
     1381 actggtcacc gtcgagccca agcagacggt cgacgcgcgc agttaccccg tcaccggtga
     1441 gcgcgcgttc gccagcctgc cggcgaacgt cggcgggcag gtgttgtacg cctgtcaggg
     1501 cgacctcgtc gacgccaacc gcgggctcgt caacttcagc gacctgctca agcggcccta
     1561 cgagcattac aagtatctgc tgaccgcgac cgagtccggg gtggtcgccc tcgatcacgt
     1621 ggtgctcgcg ctcgacctgg tgttcaccgg ctcggcgaac gacctcaacc tgctcgagtt
     1681 ccgggccctg cgatccggcg agtaccagag cctgcgcggg cgcctggagc tgatcccggt
     1741 cccgtacctg ctcgactaca gggtcgagcg caaggtctat caggagcagg tcggcgacgt
     1801 attgcgtggg atccacatcg cgccgcacgt gccccggatc ctcgcgctgt ggggggtgat
     1861 gacgcggctg cgcgcgcccg atcccgcggc ctacagcgcc aagctgcagc cggtgctcgc
     1921 gcggctgacc ccgctcgaca aggcggacct gtacgcctac ggccgcgtgc ccgccgggct
     1981 gacgagcgac gaggcgcgcg agctgctggc cgcggtcccg aagatgcacg aggagcggtt
     2041 ccagcaggcg gtggtcaagg ccgagagcgg cgacccgatc ctgctcggcg actacgaggg
     2101 cagcttcggc gccagcgtcc gcgacctcaa gcgcgtgctg ctggcggcgg cgagcgaccc
     2161 gggcaccaac gcggtgacgg tgccgcgact cttcaaagag ctgcgccgct tcatggccga
     2221 cagcgtcaac ttccgctgga tgctgctgac gcccaaggcc ccgggcccgg acggctcgcg
     2281 ctccgacggc ggctttcatg cgctcgaggg cgagggcagc atcacgcagg cggtgtggaa
     2341 ccgctggctc gacctgtccg actgggaggt gcgcgaggcg atgggcctgg tcgacgagtc
     2401 gcgctacctc gacctcttta aaaaatacgt caagcacgtc tcgcaccacc tcaagcgcga
     2461 gcgcatgttc gacgcggtga ccggcgacct gcgcaacccc gacgagaaat tcatgcaggg
     2521 cctcgagaag acgatggacc cgaaggcggg cccgaacttc cgcgccgact tgctctcgcg
     2581 gatcggcgcg tgggccctga gccaccccga cgaggagccg gcctacgccg agatcttcgc
     2641 cgactacttc ggccgcctgc gcgaggatta ttaccgccag cagaaggcgg tcgtcggccg
     2701 cgggatccag cgcatgctcg agctgctcgc cgacgaccga cgcagcgaga acccgctcgc
     2761 cccgcaagag gagcaggtcg cccgccacgc ggtcgacgtg ctgctcggca actgcgacga
     2821 caagagcgtc cgccgcgagc gtcactcgcg ggagacgctg cgcgacacgc tcgtgcagct
     2881 gtcgaagttt cgctactgag gcgccgggcg cgacacgtgc gactcgcggg agacgctcgt
     2941 gtgtcgagct tggcgcctgc gcctgcccgg cgggctcagg gggtcgtctc ggtcttcttg
     3001 acgttgatct cgacgcggcg accctcgggg ccgtcctcga ccttgacgtt gacggtaccg
     3061 tcgacgccgt cggcggcgag ctgatcgcgg atctgctgct cggcgtcggc cggggcgagg
     3121 tcatcgtcga cggcgacgat cggcagcggc ggcggctggc cggcggggat cgtggcggtg
     3181 acgatcgtcg cgcttgcgag ggccgggaag gtggtcttga gcttgtcgga gacggtgccg
     3241 gcgtccggca tcgccccgcc ccacagctcg atctcgagca cgggcgacgc gtcgtcctcc
     3301 ttcttgacgc gcaccgtcgc ggcgccgttg ccctcgccgt tgccttcgat ctgtttggcg
     3361 atcgcgtgca cggtctcggg gggtagctcg ccggcgttgc tgatcgtcac gcgcagcccg
     3421 gggttggcgg tcagctcggc cgagcgcgcc tcgatgtcct cgctgttctg acaggcaggg
     3481 agcagcgaga gaaaggagca gaggaggggg agcaggaagg gcttgaacat cggtgggtcc
     3541 ttgccagcgt ctacgcgggc gccggggaag ggtgacagcg cgggatcaga aatttttgga
     3601 aggagtacgc gcgaggatga accgggcccg cacggttggg aatcgcaggc ggtcagggta
     3661 cgttgatccg catgctcgct cgttggctct gcgtggcgct ggtcgggtcg gcgtgcacgc
     3721 gcaacgagcc ggaggtggtc gcggtggcgc cggtcgccga ggcgcctgcg ccggcccgtg
     3781 aaccgccacc acgcgccgcc agcggggtcg agctggccgc cctcgacgcc ctgctgcgtc
     3841 cgtgtcaggg cgaggaggag ggccacccgc tcggcacgct cgtctccgtc gagcggctcg
     3901 gcgcgtggaa ggacaggccg gtttacctcg cgcgcacgac cggcggtggc gatgttcgga
     3961 tgctgctgtt cgccgacacg acgggctcgt gccccgcgtt cgtgctcggg ccggaggcgg
     4021 cgcaggtccg cggtgatttc ttcggcgagg gcacgcagcg cacggcgggg ttgctgtcgc
     4081 aggccagcga cggggtccgc tgtgacaatg acgcctgccc ggtggcggtc ctgctgcgcg
     4141 acgaggacgg cgtcgtgctg gccgcggcga accccgggct cgagtgcgag acgtacacgc
     4201 tggcgagcgt gcgcctgttc gccgaccgcg actcgatcga gctgcgctgc agcggcggca
     4261 ccgtcgacac cgcgcgcgag atccagctgc tgcacgcgtt cggcagggag ctgcgcccgc
     4321 tgctcgcgtt cgacgccggg atcacgaccg tcacgctgag cgaggacgag acgcgcgagt
     4381 gcacgagccg ctccagggac ggcagctacg agatcaaggc cacgggcgcg gcgccggtgg
     4441 tcgaggtgtt cgcggtcagt gaccttcagg cggcgacgcg cgcgacctgg accttcgacg
     4501 ccgcgcagca gcgcatggtc cagggcacgc cggtgcccgt gaagttgccg gccccgcggg
     4561 cgacctgcag ggccgcgggt taggtctgcg gcagcacggc ggcggctcgc agcccgcggc
     4621 cgacgaggta ctgctcggtg ctgccggagc gggtgttctt ggcgtggtag ggcttggtct
     4681 cctggaagga ggcgcgccac tgcttgagca gctcggggaa ctggccgccc tggaacacct
     4741 tggcgacgag gtgtccgccg gggcgcaggg cctggcgggc gatctcgagg gcgcgcgtgg
     4801 cgagggcctc gctgcgaaat tggtcggtgt gcttgtcacc ggtggtctgc ggcgccatgt
     4861 cggagacgac gaggtcgaag cggacttcct ggccctcgct cgccagcacg gcgggtgacc
     4921 actcccagac atcggcgacc gcgtggtgca cgttggccgg cagggcgacg gtgacggggc
     4981 gcaggtcgat gccgacgacg aggccggtgt cgccgacctt ttgcgcggcg tactgcatcc
     5041 acgagccggg ccagcagccg aggtcga
//
LOCUS       DASWNJ010000095         4748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_284441, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000095 DASWNJ010000000
VERSION     DASWNJ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284441"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VGB85_01770"
     CDS             <1..892
                     /locus_tag="VGB85_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132767.1"
                     /translation="EPTGSPTSSPTSPATTDPTDTDEPGDTDAGGAEEVGPNYGLLTF
                     TLYPADAAGSPEQLGMAGAWRTEPFTTDDFYGVHALGLHFPRAPAAADTLELHDPQVY
                     EWGKAGTWVTLGNGVRLTAAGAGGDALACLELVADSYPVYFSGDAPIFDPSCAPDPAR
                     WVPATAYDLVVYGGENFDDQARGAAVITPAPLSVTAPAIDVFDFPLGKAGDLALAWQA
                     DAAGDRVVIRVWDQFGKQIVVHAADDGSYTIAGAELDKLAAGPATLTLAREHVSEVGL
                     AAGVLRVVTRHEVTAYPDLF"
     gene            935..2443
                     /locus_tag="VGB85_01775"
     CDS             935..2443
                     /locus_tag="VGB85_01775"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY0132768.1"
                     /translation="MQRRTFLKSAGIAASAGAAFSVLRHPRQAQAAWGDWPADKLEAM
                     IPGERQAKGVLELYLYGGMNAFDTFYTVPKWGMNQERFLNAYYPQTVNRFAQCGFSGE
                     LTEEFAEDQNGEMVHLGPWITALRARPDIVARMRVIVQRHDLLPHEGANPYALTGSRL
                     GSPRLAGVGAAIQRHFLEQPDGLRAAPYAYVLYPGVEFPTDNVRAASAVGFHPGSARP
                     LSVTVDPDSQLAMLLGRPNLVGRRAEFDAAVDHYLRSYQQRFKPGGKGTPTRSAERSN
                     YEFAHFARRDADKLQGILSGDLFMPIGGEECGANVGVDKPRMQARMAANLLTRATDRA
                     RYVQWIDGGLQPNGSGGHDTHDSHVQDASRNVSHTMQALADIINTPGEGDPNKIDLDE
                     TMVVINTEFGRTPHRQGDTGLNHWPQGMINVMIGGPLTASERGIYGAITEEEGFSQTW
                     VSPAENRMMVMMALGIYPFSSQTFAVGDVAGGVKDQLEAAKRLAQVYLGIKP"
     gene            2440..4614
                     /locus_tag="VGB85_01780"
     CDS             2440..4614
                     /locus_tag="VGB85_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132769.1"
                     /translation="MNTSRPTILLPLALALAACSDDGQTATDTKADSGADTGSATEPP
                     GPTEAGDTGDSDTPTTTTPGGDSGPNDSGQDGEPPIPDDPAAEACDAGDEAFVKRVIP
                     LVQGRKPEGMREVKLLVSMIEQLDAAGVDGRAVVVRGLASGDLYIGRWKQFLWEQLRI
                     NRVEVKSNYKCYSQTSGPNNAPDLAAYIRDNDANGSDFGQAFTMADVMESALRLDDMS
                     PLYRADMFARMARPLGGANISQEEFEVNARSNFGEIFESAYLGRRSGCLECHNSEESV
                     TYNPDPKFNHFWAIPGKFEAAIYGAAKGRPEAEIYAAFRVTGFTTDQGVRAWGMSSCG
                     SFNPGHDGDILGIPGYLGGELPAGQNLFDLDPLFKSGFATLAADGLTLADNLDVSPDQ
                     ALAYLTATNFVNNAWKEMMGYPLTLAHNFPRNEKQREILQQLTEAFVAEDYSLRAVIT
                     EIALNPYFNMDAPDACGTSTPYFMEPVFEPFSITSGDPNQRGNGVGDRVQRYSAWVLI
                     ESAMRSMWWNLPVKQIPANEWNFPAIDFLRDTGVFIKDAQNGFNGVDFNGLLAWENRL
                     ASGHNPGLSGDCTGPLGQGCSQYEWIELMLVEAFKADATIGEVAAAIKDRILGEPTIE
                     GDTELAVIAGVIGFDPSAKMKGVDTVLLEEGARRYAGLLFNTPQFMLSGVPSLDQDPD
                     QIPKFAVPGTDTKALCDHLAPAVLTDKYAWTCDAEGVKLSPK"
BASE COUNT          786 a         1836 c         1483 g          643 t
ORIGIN      
        1 cgagccgacc ggctcgccga ccagctcgcc gaccagcccg gccaccaccg acccgacgga
       61 caccgacgag cccggcgaca ccgacgccgg cggtgccgag gaggtcggcc cgaactatgg
      121 cctgctgacc ttcacgctgt acccggccga cgccgccggt tcgcccgagc agctcggcat
      181 ggccggcgcg tggcggacgg agcccttcac caccgacgac ttctacggcg tgcacgcgct
      241 gggcctgcac ttcccgcgcg cgcccgcggc cgccgacacg ctcgagctgc acgacccgca
      301 ggtctacgag tggggcaagg cgggcacctg ggtgacgctc ggcaacggcg tgcgtctgac
      361 cgccgcgggc gccggtggtg acgcgctcgc ctgtctcgag ctcgtcgccg acagctaccc
      421 ggtctacttc agcggcgacg caccgatctt cgacccgagc tgcgcgccgg acccggcgcg
      481 ctgggtgccg gcgaccgcgt acgacctcgt cgtctacggc ggcgagaatt ttgacgatca
      541 ggcccgtggc gccgccgtga tcacgccggc ccccctgtcc gtcaccgccc cggcgatcga
      601 cgtgttcgac ttcccgctcg gcaaggccgg ggacctcgcg ctcgcctggc aggccgacgc
      661 cgccggcgac cgcgtcgtca ttcgcgtgtg ggatcaattc ggcaagcaga tcgtcgtgca
      721 cgccgccgac gacggcagct acacgatcgc cggtgccgag ctcgacaagc tcgccgccgg
      781 accggccacc ctgacgctcg cgcgcgagca cgtcagcgag gtcggcctcg ccgcgggcgt
      841 ccttcgtgtc gtcacccgcc acgaagtcac cgcctacccc gacctcttct agtccttgcc
      901 cttaactcgc ccaccccccc gagtgagacc ccccatgcag cgcagaacct tcctcaagtc
      961 cgctggcatc gccgcctcgg ccggcgccgc cttcagcgtc cttcgtcacc cacgccaggc
     1021 ccaggccgcg tggggtgact ggccggccga caagctcgag gcgatgatcc ccggcgagcg
     1081 ccaggccaag ggcgtgctcg agctctacct ctacggtggc atgaacgcct tcgacacctt
     1141 ctacacggtg ccgaagtggg gcatgaacca ggagcgcttc ctcaacgcct actacccgca
     1201 gaccgtcaac cgcttcgcgc agtgcggctt ctcgggtgag ctgaccgagg agttcgccga
     1261 ggaccaaaac ggcgagatgg tccacctcgg cccatggatc accgcgctgc gggcccggcc
     1321 ggacatcgtc gcgcgcatgc gcgtcatcgt gcagcgtcac gacctcctgc cgcacgaggg
     1381 cgccaacccc tacgcgctga ccggcagccg cctcggctcc ccgcgcctcg cgggcgtcgg
     1441 cgcggcgatc cagcgccact tcctcgagca gcccgatggc ctgcgcgcgg cgccttacgc
     1501 ctacgtgctc taccccggcg tcgagttccc gaccgacaac gtgcgcgccg cctcggccgt
     1561 cggctttcac cccggctccg cgcgcccgct cagcgtcacc gtcgaccccg actcgcagct
     1621 cgctatgctg ctcgggcgcc cgaacctcgt cggacgccgc gccgagttcg acgccgccgt
     1681 cgaccactac ctgcgctcgt atcaacagcg cttcaagccc ggcggcaagg gcacgccgac
     1741 gcgcagcgcc gagcgcagca actacgagtt cgcccacttc gctcgccgcg acgccgacaa
     1801 gctgcagggc atcctctccg gtgacctgtt catgccgatc ggcggcgagg agtgcggcgc
     1861 caacgtcggc gtcgacaagc cgcgcatgca ggcgcgcatg gccgccaacc tgctgacccg
     1921 cgcgaccgac cgcgcccgct acgtgcagtg gatcgacggc ggcctccagc ccaacggctc
     1981 gggcggccac gacacccacg acagccacgt gcaggacgcc tcgcgcaacg tcagccacac
     2041 gatgcaggcg ctcgccgaca tcatcaacac ccccggcgag ggcgacccca acaagatcga
     2101 cctcgacgag acgatggtcg tcatcaacac cgagttcggc cgcaccccgc accggcaagg
     2161 cgacaccggc ctcaatcact ggccgcaggg catgatcaac gtgatgatcg gcggcccgct
     2221 caccgcgagc gagcgcggca tctacggcgc gatcaccgag gaagaggggt tctcccagac
     2281 ctgggtcagc cccgcagaga atcgcatgat ggtgatgatg gccctcggca tctacccatt
     2341 tagctcgcag acctttgccg tcggcgacgt cgccggcggc gtcaaggatc agctcgaggc
     2401 cgccaagcgc ctggcgcagg tctacctggg gatcaagcca tgaacacctc ccgacccacg
     2461 atcctcctgc ccctcgccct cgccctcgcg gcctgcagcg acgacggcca gaccgccacc
     2521 gataccaagg ccgacagcgg cgcggacacg ggcagcgcga ccgagccgcc cgggccgacc
     2581 gaggccggcg ataccggcga cagcgacacg ccgacgacca ccacgcccgg cggcgacagc
     2641 ggccccaacg acagcggtca agacggcgag ccgccgatcc ccgacgaccc cgccgccgag
     2701 gcctgcgacg ccggcgacga ggcctttgtt aaacgcgtga tcccgctggt ccagggtcgc
     2761 aagcccgagg gcatgcgcga ggtcaagctg ctcgtcagca tgatcgagca gctcgacgct
     2821 gccggcgtcg acggccgcgc cgtcgtcgtc cgcggcctcg cctccggcga cctctacatc
     2881 ggccgctgga agcagttcct gtgggagcag ctgcggatca accgcgtcga ggtcaagtcg
     2941 aactacaagt gttactcgca gaccagcgga ccgaacaacg ccccggacct cgccgcgtac
     3001 atccgcgaca acgacgccaa cggcagcgac ttcggccagg cgttcacgat ggccgacgtc
     3061 atggagagcg cgctgcgcct cgatgacatg agcccgctgt accgcgccga catgttcgcc
     3121 cgcatggcgc gtccgctcgg cggcgcgaat atttctcagg aggaattcga ggtcaacgcg
     3181 cgctcgaact tcggcgagat cttcgagtcc gcgtacctcg ggcgacgcag cggctgcctc
     3241 gagtgtcaca acagcgagga gtcggtcacc tacaaccccg accccaagtt caaccacttc
     3301 tgggcgatcc ccggcaagtt cgaggctgcg atctacggcg ccgccaaggg ccgccccgag
     3361 gccgagatct acgcggcctt ccgcgtcacc ggcttcacca ccgatcaggg cgtgcgcgcc
     3421 tggggcatga gctcgtgcgg ctccttcaac cccgggcacg acggcgacat cctcggcatc
     3481 cccggatacc tcggcggcga gctgcccgcg ggtcagaacc tgttcgacct cgacccgctg
     3541 ttcaagtccg gcttcgccac cctggcggcc gacggcctga cgctcgcgga caacctcgac
     3601 gtctcgcccg accaggcgct cgcgtatctg accgcgacca acttcgtcaa caacgcgtgg
     3661 aaggagatga tgggctaccc gctgacgctg gcccacaact tcccgcgcaa cgagaagcag
     3721 cgcgagatcc tgcaacagct gaccgaggcc ttcgtcgccg aggactactc cctgcgcgcc
     3781 gtgatcaccg agatcgcgct caacccctac ttcaacatgg acgccccgga cgcgtgcggc
     3841 acctcgacgc cgtacttcat ggagcccgtg ttcgagccgt tctcgatcac cagcggcgat
     3901 ccgaaccagc gcggcaacgg cgtcggcgac cgcgtccagc gctacagcgc gtgggtcctg
     3961 atcgagtcgg cgatgcgcag catgtggtgg aacctgccag ttaaacagat ccccgcgaac
     4021 gagtggaact tcccggcgat cgacttcctg cgcgacaccg gcgtgttcat caaggacgcc
     4081 cagaacggct tcaacggggt cgacttcaac ggcctgctcg cgtgggagaa ccgcctcgcg
     4141 tccgggcaca accccggcct gtcgggcgac tgcaccggcc cgctcggcca gggctgctcg
     4201 caatacgagt ggatcgagct gatgctcgtc gaggccttca aggccgacgc gaccatcggc
     4261 gaggtcgcgg ccgcgatcaa ggaccgcatc ctcggcgaac cgacgatcga gggtgacacc
     4321 gagctcgccg tcatcgccgg cgtcatcggc ttcgacccct ccgccaagat gaagggcgtc
     4381 gacaccgtcc tcctcgagga gggcgcccgc cgctacgccg gcctgctgtt caacaccccg
     4441 cagttcatgc tctccggcgt gccctcgctc gaccaggacc ctgaccagat cccgaagttc
     4501 gcggtccccg gcaccgacac caaggccctg tgcgaccacc tcgccccggc cgtactgacg
     4561 gacaagtacg cgtggacctg cgacgccgag ggcgtcaagc tctcgccgaa gtgaccgcgc
     4621 gtcagcgtcc gccggtcggc accaccgagc cgcgcggacg cagctcggag atcctgcgct
     4681 cgacgagcgt gcgctccggc gcctccggct cgggcagcgc caggtaggcc tgccagtagc
     4741 gcgtcgcg
//
LOCUS       DASWNJ010000096         2516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_602540, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000096 DASWNJ010000000
VERSION     DASWNJ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602540"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1993)
                     /locus_tag="VGB85_01785"
     CDS             complement(<1..1993)
                     /locus_tag="VGB85_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0132770.1"
                     /translation="MRVEPKNIVRRLNTTCTAMLEAAVSQAASGRYYEIAPEMLLLAL
                     LGEGDTDVRAIFSHAEQDIARLRQRVQRTTDLLRSGNPGRPVFGENVFQWIEDAWVFA
                     SLQYGVDEIRSGHLLLMFASRGHRYCAEIFPELNALPVEALRRELEAITANTREAVQK
                     PATGAGPANTPAAAADSALRRFTSSYTEKARAGKIDPIFGRHAEIRQLAEILARRRKN
                     NPIIVGEPGVGKTALVEGLALAIAAGDVPSPLREVELLELDMGLLQAGAGVRGEFEKR
                     LKQVIDEVKSSPRPIVLFIDEAHTIVGKEADAANLLKPALARGELRTIAATTWSEYKK
                     YFEKDAAFARRFQPVKVDEPSEEQALVMLRGLRTTYEQAHGVTIRDEALTAAVSLSHR
                     YIAGRQLPDKAVDLLDTASTRVRIEHEAKPEALVMHEQQVMALERELALLERERAEVD
                     GIDAARVTEVQARLAAARDALLTLQGRWQSEREALIEVETARKAMRDGGGDAARQVKR
                     AADERLLSLAGDDRLIHAEVDADAVARVVATWTGIPVGKMRSDSLGALLTLEDRLGAR
                     VRGQSRAVQVAAETLRIAAARVQNPNTPLGVLLFVGPSGVGKTEMATALADLIYGGER
                     FMTVINMSEFQEKHSLSRLIGSPPGYVGYGEGGVLTEAVR"
     gene            complement(2088..>2516)
                     /gene="tssD"
                     /locus_tag="VGB85_01790"
     CDS             complement(2088..>2516)
                     /gene="tssD"
                     /locus_tag="VGB85_01790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HEY0132771.1"
                     /translation="RDLGRDKTIECLVFRDNVRTAREMGTRAAVGRRVHEPIEVIKRI
                     DASSPLLAKALCKNEKVEGTFKFYRPSPKGDGTTEQFFTIEIKEGRIDGIERHSPNAI
                     DPAASKAPPLETVRFVYHTIRWVYEPSGAEYVDSWGTSEA"
BASE COUNT          322 a          913 c          868 g          413 t
ORIGIN      
        1 ggcgcaccgc ctcggtcagc acgccgccct cgccgtagcc gacatagccc ggcggcgagc
       61 cgatcagccg cgacagcgag tgcttctcct ggaactcgga catgttgatg accgtcatga
      121 agcgctcgcc gccatagatg aggtccgcga gcgccgtcgc catttcggtc ttgccgacgc
      181 cgctcggccc gacgaacagc agcacgccga gcggcgtgtt cgggttctgc acccgggccg
      241 cggcgatgcg cagggtctcg gccgcgacct ggacggcccg gctctgcccg cgcacccgcg
      301 cgccgagccg gtcctcgagg gtcagcagcg ccccgagcga gtcgctgcgc atcttcccga
      361 ccggaatgcc ggtccacgtg gcgaccaccc gcgcgaccgc gtcggcgtcg acctccgcgt
      421 ggatcaggcg atcgtcgccg gccaggctca gcagacgctc gtccgcagcg cgcttgacct
      481 ggcgcgccgc atcgccgccg ccgtcgcgca tcgccttgcg ggccgtctcg acctcgatca
      541 gcgcctcgcg ctcggactgc cagcggcctt gcagggtcag cagcgcgtcc ctggcggccg
      601 cgaggcgcgc ctgcacctcg gtgacgcggg cggcgtcgat gccgtcgacc tctgcgcgct
      661 cacgctcgag cagcgccagc tcgcgctcga gcgccatcac ctgctgctcg tgcatcacca
      721 gcgcttcggg cttggcctcg tgctcgatgc gcacgcgagt gctcgcggtg tcgagcaggt
      781 cgacggcctt gtccggcagc tggcgtccgg cgatgtagcg gtgcgacagc gacaccgcgg
      841 cggtcagcgc ctcgtcgcgg atcgtcacgc cgtgggcctg ctcataggtc gtccgcagcc
      901 cgcggagcat cacgagggcc tgctcttccg acggctcgtc gaccttgacc ggctggaacc
      961 ggcgcgcgaa cgcggcgtcc ttctcgaagt acttcttgta ctcggaccag gtcgtcgccg
     1021 cgatcgtgcg cagctcgccg cgcgcgagcg ccggcttgag caggttggcg gcgtcggcct
     1081 ccttgccgac gatcgtgtgc gcctcgtcga tgaacagcac gatcggccgc ggcgagctct
     1141 tgacctcgtc gatcacctgc ttgaggcgct tctcgaactc gccgcgcacg ccggcgccgg
     1201 cctgcagcag gcccatgtcg agctcgagca gctcgacctc ccgcagcggc gacggcacgt
     1261 cgcccgccgc gatcgccagg gccagcccct cgaccagcgc cgtcttgccg acgcccggct
     1321 cgccgacgat gatcgggttg ttcttgcgtc gccgcgccag gatctcggcg agctggcgga
     1381 tctcggcgtg acggccgaag atcgggtcga tcttgccggc gcgggccttc tccgtgtagg
     1441 agctggtgaa gcggcgcagc gccgagtcgg cggcggccgc gggcgtgttc gccggtcccg
     1501 cgccggtcgc tggcttctgg accgcctcac gggtgttcgc ggtgatcgcc tcgagctcgc
     1561 ggcgaagggc ctcgactggc agcgcgttga gctcggggaa gatctcggcg cagtagcggt
     1621 ggccccggct ggcgaacatc agcagcaggt gcccgctgcg gatctcgtcg acgccgtatt
     1681 gcagggacgc gaacacccac gcatcctcga tccattgaaa gacgttctcg ccgaacaccg
     1741 gccggcccgg gttgccgctg cgcagcaggt ccgtcgttcg ctggacccgc tggcgcagcc
     1801 gcgcgatgtc ctgctcggcg tggctgaaga tcgcgcggac gtcggtgtcc ccctcgccga
     1861 gcagggccag cagcagcatc tcgggggcga tctcgtagta gcgcccgctc gccgcctggg
     1921 agacggcggc ctccagcatc gcggtgcagg tggtattcag ccggcggacg atgttcttcg
     1981 gttcgacgcg cattcgggcc tcggcttcat gacatacaga cggggccgcg ccgtctcggc
     2041 tcggccccgc ctcgtgctca tcaggtcatt cagggatagg tcgcggctca ggcctcggag
     2101 gtgccccagc tgtcgacgta ctcggcgccg gacggctcat agacccagcg gatcgtgtga
     2161 tagacgaagc gcaccgtctc gagcggcggc gccttcgagg ccgccgggtc gatcgcgttc
     2221 ggcgagtggc gctcgatgcc gtcgatgcgc ccctccttga tctcgatcgt gaagaactgc
     2281 tcggtggtcc cgtcgccctt ggggctcggg cgatagaact tgaaggtgcc ctccaccttc
     2341 tcgttcttgc acagcgcctt cgccagcagc ggcgagctgg cgtcgatgcg cttgatcacc
     2401 tcgatcggct cgtggacgcg acgcccgacg gccgcccggg tgcccatttc acgcgccgta
     2461 cggacattgt cgcggaagac gaggcactcg atcgtcttgt cgcgccccag gtcgcg
//
LOCUS       DASWNJ010000097         2248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_107844, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000097 DASWNJ010000000
VERSION     DASWNJ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107844"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VGB85_01795"
     CDS             <1..453
                     /locus_tag="VGB85_01795"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY0132772.1"
                     /translation="VELGGAFKNVIALGAGVCDGAGLGQNARAALITRGLAEIGRLCH
                     ALGAHPATLSGLAGLGDLVLTCTGGLSRNRRVGLALGQGHKLDDILREMGMVAEGINN
                     TLSAHRLAQREGVEMPITAMMHAILYEGISVRTAVHALMQRDLKPERD"
     gene            653..>2248
                     /locus_tag="VGB85_01800"
     CDS             653..>2248
                     /locus_tag="VGB85_01800"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0132773.1"
                     /translation="MSNIEATPFGDKYVLVEHIATGGMAEIYRAQYSGIEGFAKELVI
                     KRLREEFAARPSVVQMFLDEARVAATLTHQNVVHTYDLGELQGEYYIAMELLKGQELV
                     AVLRRAVKTGHMLPLQIALGIVMQALEGLHYVHTRVDDRGRPMGLCHRDMNPTNIHVG
                     YDGTCKIVDFGIAATRATLQAGGDGQFAGKLAYMAPEQVLGHELDLRADIFALGVVLY
                     EMCLSRRLFRGVPEEVAERIVEGDIPAPTFVNPEFPPALEAVIMKALEVDPAERYQNC
                     DHMFRDLEKFTEQASIRCTPRMISAYMSEMFGEGAPAEVNYDDQYDDLVDEALDFDKF
                     DSLEANKSDAAPDWAKSFDNPDASGARNKRSMTIGNLASMLGPEFEQQRQQMVAEHET
                     GPSPTTAPQTGSQGRGRGRHRPVGAAASGASAGSGASAGSGSGESEGPKTRRPASTAI
                     KQSGSGGANKPGSGKQGVAPLGRAPTAGRPVVRAGQSITTGALSSIDTGINAGSFGHG
                     VMAEQTKKGGAALTWLLVVLGLGG"
BASE COUNT          362 a          754 c          829 g          303 t
ORIGIN      
        1 gtcgagctcg gtggggcgtt caagaatgtc atcgcgctcg gggctggcgt gtgcgacggg
       61 gcggggctcg ggcaaaacgc ccgggccgcg ctgattaccc gcgggctcgc ggagatcggc
      121 cgtctgtgcc acgcgctcgg ggctcacccg gcgacgctct cggggctggc cggcctcggc
      181 gatctcgtgc tgacctgcac gggcggtctg tcgcgtaacc ggcgggtcgg tcttgcgctc
      241 ggtcagggcc acaagctgga cgacatcctg cgcgagatgg gcatggtcgc ggagggcatc
      301 aacaacacgc tcagcgccca ccgactggcg caacgcgagg gcgtcgagat gccgatcacg
      361 gcgatgatgc acgcgatcct ctacgagggg atcagcgttc gaaccgcggt ccacgcgctc
      421 atgcagcgag atctcaaacc cgagcgcgac taggcgcggc agggcgcccg ggacgccggg
      481 ggcgcggacg gacgcggtca aggagcggtc gggtgtgggc cgggcgaggc cgcgagggcc
      541 attttggcgg ggcgaccccg ggacaccgcc gatcgaccct gccaccgggt ccgcgccgcg
      601 ggcgacggcg gggcttggcg ctggatcggg gatccggtag tctcgtgcga ccttgagcaa
      661 cattgaggcc acgccattcg gcgacaaata tgtgctggtc gagcacatcg ctaccggagg
      721 catggcggag atctaccgcg cgcagtactc cggcatcgag ggctttgcca aggagctcgt
      781 catcaagcgg ctgcgcgagg agttcgcggc gcgtccgagc gtcgtgcaga tgttcctcga
      841 cgaggcccgc gtcgcggcga cgctgaccca ccagaacgtc gtccacacct acgacctcgg
      901 cgagctgcag ggcgagtact acatcgcgat ggagctcctg aagggtcagg agctggtcgc
      961 ggtgctgcgt cgcgccgtca agaccgggca catgctgccg ctgcagatcg cgctcggcat
     1021 cgtgatgcag gcgctcgagg gcctgcacta cgtgcacacg cgggtcgacg accgcggccg
     1081 tccgatgggc ttgtgtcacc gcgacatgaa cccgaccaac atccacgtcg gctacgacgg
     1141 cacctgcaag atcgtcgact tcggcatcgc ggcgacccgc gcgaccttgc aggccggcgg
     1201 cgacgggcag ttcgcgggca agctggcgta catggccccg gagcaggtcc tcggccacga
     1261 gctcgacctg cgcgccgaca tcttcgcgct cggcgtcgtg ctctacgaga tgtgcctgtc
     1321 gcggcgtctg ttccgcggcg tgcccgagga ggtcgcggag cgcatcgtcg agggcgacat
     1381 cccggcgccg accttcgtca accccgagtt cccgccggcg ctcgaggccg tgatcatgaa
     1441 ggcgctcgag gtcgacccgg ccgagcgcta tcaaaattgc gaccacatgt tccgtgacct
     1501 cgagaagttc acggagcagg cgagcatccg ctgcacgccg cggatgatca gcgcctacat
     1561 gagcgagatg ttcggcgagg gcgcgccggc cgaggtcaac tacgacgacc agtacgacga
     1621 cctcgtcgac gaggccctcg acttcgacaa gttcgactcg ctggaggcca acaagagcga
     1681 cgcggccccc gactgggcca agagcttcga caacccggac gcctcgggcg cgcgcaacaa
     1741 gcggtcgatg acgatcggca acctcgcgtc gatgctcggg ccggagttcg agcagcagcg
     1801 ccagcagatg gtcgccgagc acgagaccgg gccgtcgccg accaccgcgc cacagacggg
     1861 ctcgcaaggg cgcgggcgcg gccggcatcg tccggtcggc gcggcggcat cgggcgcatc
     1921 ggcgggatcg ggcgcatcgg cgggatcggg gagcggcgag tccgagggcc cgaagacgcg
     1981 ccggccggcc tcgacggcga tcaagcagag cggctccggc ggcgcgaaca agccgggctc
     2041 gggcaagcag ggcgtcgcgc cgctgggtcg cgcgccgacg gcgggccgcc cggtggtccg
     2101 cgcggggcag tcgatcacga ccggcgcgct gtcgtcgata gacaccggca tcaacgccgg
     2161 cagcttcggt cacggcgtca tggccgaaca gaccaagaag ggcggcgcgg cgctcacgtg
     2221 gctgctcgtc gtcctgggcc tcggtggc
//
LOCUS       DASWNJ010000098         7460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_672609, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000098 DASWNJ010000000
VERSION     DASWNJ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7460
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672609"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..715
                     /gene="hpt"
                     /locus_tag="VGB85_01805"
     CDS             125..715
                     /gene="hpt"
                     /locus_tag="VGB85_01805"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEY0132774.1"
                     /translation="MRQADNDVWTGGLVDDLSKTLLSEAQIQQRVAELGRQLQTDYAD
                     KDPLLVGILSGSFPFIADLVRAVNLPLAVDFMAVSSYGDRTSSSGVVRILKDLNSSIA
                     GRHVIIIEDIVDTGLTLDYLVQNLQTRHPASLKVCALLDKKEARKREVEVAYVGFPCP
                     NEFVVGYGLDYRNRYRNLPFIGVLRPEVYQTHQGAE"
     gene            718..1806
                     /gene="add"
                     /locus_tag="VGB85_01810"
     CDS             718..1806
                     /gene="add"
                     /locus_tag="VGB85_01810"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEY0132775.1"
                     /translation="MSKLSLAQIEKLPKTDLHVHLDGSMRIETLIELAKEQGVPLPAS
                     TPEGMKEAMGLGRNTGSLVEYLKAFDLTLQVLQDEESLYRVAYELAVDCAAENVRYME
                     VRYSPMLHTRRKLKLTKVVETVLRGLHDAQADHNIESNLIVCGIRNISPESSMRMAEL
                     VVAYKNRGVVGFDLAGAEYDYPPKDHQEAFALVRQNNINVTIHAGEAYGPASIAQALH
                     VCGAHRIGHGCRLREDGDLLRYVVDHRIPLECCPSSNVQTGAVPSIAEHPLRLYFNLG
                     ARITVNTDNRLITDTTVSRELHHLHTQLDFDWTELRQVILNGFKSAFLPYHEKRRFMR
                     AISAELDEFARGLEPLEPLDQVEQQPAA"
     gene            complement(1813..2223)
                     /locus_tag="VGB85_01815"
     CDS             complement(1813..2223)
                     /locus_tag="VGB85_01815"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HEY0132776.1"
                     /translation="MPAPPSIAGDVLRDRLSNERTLLSWLRTSIALMGFGLVVARFGL
                     FLKTMIAMQAGSSAVELNTQADRSHLIGAGLLLMGSLAALVGWQRSRAYGRIIDASSA
                     GPSHRTLTITALAVAGLGFTLAVYVAILERVVHH"
     gene            complement(2289..3182)
                     /locus_tag="VGB85_01820"
     CDS             complement(2289..3182)
                     /locus_tag="VGB85_01820"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEY0132777.1"
                     /translation="MDGPRYASAAAFKQALEQRLKDLGDLDRRRQLLVYDRFLARTTI
                     ALGDAVTLKGGLGLEVRLRRARTTRDVDLRMSGSPADLLPRLQTLGRLDLGDFMSFDV
                     EPHPSHPVLTGAGVRYHGQRFTVRCSLVRKLYARPFGLDIGFADPMLGPPDRIAAADV
                     LAFAGIPAPILQVYPVVTHIAEKLHAYTLPRPTGINSRLKDLPDLALLASAGELLAAH
                     THEALAQTFAFRATHAIPHRFPDPPNEWRDRYERMASDDRLPWRTLAAVTSAARDFLE
                     PVLAGLRDSRWSPAGWAWRGS"
     gene            complement(3198..3755)
                     /locus_tag="VGB85_01825"
     CDS             complement(3198..3755)
                     /locus_tag="VGB85_01825"
                     /inference="COORDINATES: protein motif:HMM:NF020962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEY0132778.1"
                     /translation="MSQVADRPSWDALFNVALTQDGLFTTQQAAESGYSRPLLARYLQ
                     SGKVERVMHGIYRLVHYPCSESEQLTMLWLWSGREGVFSHETALFLFDLSDALPSRIH
                     LTLPNSWRRRRLRVPEVLRLHHADLPAADRTWVGAVQVTTPRRTLLDCNSDHVAPDLV
                     EQAIEQALARGMVTPKELSAIALHP"
     gene            complement(3816..4733)
                     /gene="rimK"
                     /locus_tag="VGB85_01830"
     CDS             complement(3816..4733)
                     /gene="rimK"
                     /locus_tag="VGB85_01830"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HEY0132779.1"
                     /translation="MKIGILSRKASLYSTSRLKEAAQKRGHEVYVIDYLRCYMNITSH
                     KPQVAYQGRQLEGFDAVIPRIGASNTFYGTAVVRQFEMMGVFSPNSSIAISRSRDKLR
                     CMQILAAKGIGLPVTGFAHSIKDIDGLVAIVGGAPLVVKLLEGTQGIGVVLAETKQAA
                     TSVIEAFRGLHVNILVQEFIKEAEGADIRAFVVGDRVIAAMKRQGAKGEFRSNLHRGG
                     HAEKIKLSPEERSTAVRAAQILGLKIAGVDILRSNHGPVVMEVNSSPGLEGIEKTSEV
                     DVADNIIQFLEKKNEAILADGKPNDRNNY"
     gene            complement(4730..5215)
                     /locus_tag="VGB85_01835"
     CDS             complement(4730..5215)
                     /locus_tag="VGB85_01835"
                     /inference="COORDINATES: protein motif:HMM:NF017434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HEY0132780.1"
                     /translation="MATMLRGESDALVVIGWREWVCLPDLGITMLKAKVDTGARTSAL
                     HAFAVERVEHERVRFQVHPHQRDDVAAITAEADLVDERLVRSSSGVAEMRPVVRTRVE
                     LGGQTFEAEFTLTDRALLGFRMLLGREALRRRFIVDPGGSYRCGGRRASFARPRKQKP
                     T"
     gene            complement(5522..6061)
                     /locus_tag="VGB85_01840"
     CDS             complement(5522..6061)
                     /locus_tag="VGB85_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132781.1"
                     /translation="MTLSIRNTLRSLSLLAMTAAFLPGCDSEEYEALGLSSEEIDAMS
                     EEELDELAALEDDDDLEHSVDPRARHIAAPLPPNDPDVGPDDLTAPAATGHTDHFDVP
                     TTLWAPHFTHTGPNGIDVSDTLWAPRFTHAESAVEIVEGIAIPVHPTHDDQALALGQL
                     GLPDPGCKPKTEYAQLSNG"
     gene            6289..6705
                     /locus_tag="VGB85_01845"
     CDS             6289..6705
                     /locus_tag="VGB85_01845"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY0132782.1"
                     /translation="MTEGHRSIQPPGWPRPRGYSNGMAARGELLAVAGMIGWDAQERL
                     VGDEFVPQFRQALANVCDVVRAAGGQPQHIVSLTLYVTDKQAYIAGLREVGEAYRALL
                     GKHYPAMALVQVAALLEDRAKVEIQALAVLPTSEAP"
     gene            6702..>7460
                     /locus_tag="VGB85_01850"
     CDS             6702..>7460
                     /locus_tag="VGB85_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY0132783.1"
                     /translation="MTDTSPDLRPLPLPEPRSFRYEERSRVGVITLTRPDRFNALTFE
                     VYRELTDLFLVVDRRSEEPEGARALVLQGEGRAFCSGGDVEDIITRLFARDTEGLLRF
                     TRMTGELIHAMRRCKLPIVASVKRVAAGAGAVMALASDLRVMGQGSFFSFLFPQVGLS
                     GADMGASWLLPRVVGLGNASEILLIGDRVPADRCLQMGLANRVVPDEPDRDAVDRAAF
                     ELAARIAGGPHFANRMTKTMLQQEQDMHFTDAIEA"
BASE COUNT         1198 a         2380 c         2707 g         1175 t
ORIGIN      
        1 ctcgatcgtg agggtgacga gctcgccgcg cggctgggtg aacgtgtgga caggcatcgc
       61 ggcgagccta gcctcacgcg gcgacggtct caaggcgcgc gggtttgtgg gtatgctccg
      121 cgcgatgagg caggcagaca acgacgtgtg gaccgggggg ctggtcgacg acctctcgaa
      181 gacgctgctc agcgaggcgc agatccaaca gcgcgtggcc gagctcgggc gccagctgca
      241 aaccgactac gcggacaagg acccgctgct ggtcgggatc ctcagcggca gcttcccgtt
      301 catcgccgac ctggtgcgcg cggtgaacct gccgctcgcg gtcgacttca tggcggtgtc
      361 ctcgtacggc gaccgcacgt cctcgtcggg cgtggtccgg atcctcaagg acctcaacag
      421 ctcgatcgcc gggcgccacg tgatcatcat cgaggacatc gtcgacaccg ggctgaccct
      481 cgattacctg gttcagaacc tccagacccg tcacccggcg tcgctcaagg tgtgcgcgct
      541 gctcgacaag aaggaggccc gcaagcgcga ggtcgaggtc gcctacgtcg gcttcccgtg
      601 cccgaacgag ttcgtggtcg gctacggcct cgactaccgc aaccgctacc gcaacctccc
      661 gttcatcggc gtgctgcggc ccgaggttta ccagactcat cagggggcgg agtgatcgtg
      721 tccaagctgt cgctggcgca gatcgagaag ctgccgaaga ccgacctgca cgtgcacctc
      781 gacggctcga tgcgcatcga gacgctgatc gagctggcga aggagcaggg cgtgccgctg
      841 ccggcgagca cgcccgaggg catgaaggag gcgatgggcc tcggccgcaa caccggcagc
      901 ctcgtcgagt acctcaaggc cttcgacctg acgctgcagg tgctgcagga cgaggagtcg
      961 ctgtaccgcg tcgcctacga gctggcggtc gactgcgcgg cggagaacgt gcgctacatg
     1021 gaggtgcgct actccccgat gctgcacacg cggcgcaagc tcaagctgac caaggtggtc
     1081 gagacggtgc tgcgcgggct gcatgacgcg caggccgacc acaacatcga gagcaacctg
     1141 atcgtgtgtg ggatccggaa catctcgccg gagtcctcga tgcggatggc cgagctggtc
     1201 gtcgcgtaca agaaccgcgg cgtggtcggc ttcgacctcg ccggcgccga gtacgactat
     1261 ccacccaagg accaccagga ggcgttcgcg ctggtccgcc agaacaacat caacgtgacg
     1321 atccacgccg gcgaggccta cggcccggcg tcgatcgccc aggcgctgca cgtgtgcggc
     1381 gcgcatcgca tcggccacgg ctgtcgcctg cgcgaggacg gtgacctgct gcgctacgtc
     1441 gtcgaccacc gcatcccgct cgagtgctgc ccatcgtcga acgtgcagac cggcgcggtg
     1501 cccagcatcg ccgagcaccc gctgcgcctg tacttcaacc tcggggcgcg gatcacggtc
     1561 aacaccgaca accgcctgat caccgacacg acggtgtcgc gcgagctgca ccacctgcac
     1621 acgcagctcg acttcgactg gaccgagctg cgtcaggtaa ttttaaatgg ttttaaaagt
     1681 gccttcttgc cgtatcacga gaagcgccgg ttcatgcgcg cgatctcggc ggagctcgac
     1741 gagttcgcca gggggctcga gccgcttgag ccgctcgatc aggtcgaaca acagccggcg
     1801 gcctgagccg cgtcagtggt gcacgacacg ctcgaggatc gccacgtaga cggcgagcgt
     1861 gaagccgagg ccggcgaccg ccagcgcggt gatcgtcagc gtgcggtgtg acgggcccgc
     1921 gctcgaggcg tcgatgatgc ggccgtacgc gcgcgagcgc tgccagccga cgagcgccgc
     1981 gaggctgccc atcagcaata gcccggcgcc gatcaggtgc gagcggtcgg cctgcgtatt
     2041 cagctcgacc gcgctggacc ccgcctgcat ggcgatcatc gtcttgagga acagaccgaa
     2101 gcgggcgacg acgagaccga agcccatcag cgcgatcgag gtgcgcagcc aggacagcag
     2161 ggtgcgctcg ttggagaggc ggtcacgcag gacgtccccg gcgatcgaag gcggggcagg
     2221 catgtcgcga agatgtcaca gagcagccga cgcgcaagcg cagcgtcgaa gccggccctc
     2281 atcgcgcgct acgagcctcg ccaggcccac cccgccggcg accatcgtga gtcgcgcagg
     2341 cccgcgagca ccggctcgag gaagtcgcgc gcggcggagg tgacggcggc gagcgtccgc
     2401 cacgggagtc ggtcgtcgga tgccatgcgc tcgtagcgat cgcgccactc gttcggaggg
     2461 tcagggaatc ggtgtgggat cgcgtgcgtc gctcgaaagg caaaggtctg cgcgagggct
     2521 tcatgggtgt gcgcggcgag gagctcacca gcgctggcga ggagcgcgag gtcggggaga
     2581 tccttgagcc gggagttgat acccgtcggt cgcggcagcg tgtacgcgtg cagcttctcg
     2641 gcgatgtgag tcacgaccgg gtagacctgc aggatcggcg cggggatgcc agcgaaggcg
     2701 aggacatcgg cggcggcgat ccggtccggc gggcccagca tcggatccgc gaagccgatg
     2761 tcgaggccaa aggggcgcgc gtagagcttg cggacgagcg agcagcggac agtgaagcgc
     2821 tggccgtgat agcgaacgcc agcgccggta agcaccgggt ggctcggatg tggctcgaca
     2881 tcgaaggaca tgaagtcgcc gaggtcgagg cgcccgagcg tctgcaggcg ggggagcagg
     2941 tccgcgggcg agccggacat gcgcaggtcg acgtcgcggg tggtgcgggc gcggcgaagc
     3001 cggacctcga ggcccaggcc gcccttgagg gtgactgcgt cccccaaggc gatcgtcgtc
     3061 cgggcgagga atcggtcgta caccagaagc tgacgccgac ggtcgaggtc gccgaggtcc
     3121 ttgagtcgtt gctcgagcgc ctgcttgaac gcggcggcgg atgcgtatcg tgggccgtcc
     3181 atggccaact ccttgtttca tgggtgaaga gcgatcgcgg agagctcctt cggagtcacc
     3241 atgccgcgcg cgagcgcctg ctcgatcgcc tgttccacga ggtcgggcgc gacgtggtca
     3301 ctgttgcagt cgagcagcgt tcggcgtggc gtcgtcacct ggacagcacc cacccaggtc
     3361 ctgtcggccg ccggcaggtc ggcgtgatgc aggcgcagga cctcggggac tcgcagtcga
     3421 cgacgtcgcc agctgtttgg aagggtcagg tggatccggg acggcagcgc gtccgagagg
     3481 tcgaacagga agagcgccgt ttcatgggag aacacgccct cacgtccgga ccatagccac
     3541 agcatcgtga gctgctccga ctcgctgcac gggtagtgca ccagacggta gatgccgtgc
     3601 atgacgcgct cgaccttgcc cgactgcagg tagcgggcga gcagcggccg tgagtagccg
     3661 gactcggccg cctgctgcgt ggtgaacagg ccgtcctggg tgagcgccac gttgaacagg
     3721 gcgtcccagc ttgggcgatc tgcgacctgg ctcacgcaca aatgttaact cagggctaac
     3781 ttttgtgcag cccggagcga atttgcccgt cggcgtcagt agttgttgcg gtcgtttggt
     3841 tttccgtcgg cgaggatggc ttcgttcttc ttttcgagga actggatgat gttgtcggcg
     3901 acgtcgacct cggaggtctt ttcgatgcct tcgaggcccg gggaggagtt gacctccatg
     3961 acgacggggc cgtggttgga gcgcaggatg tcgacgccgg cgatcttgag gccgaggatc
     4021 tgggcggcgc ggacggcggt gctgcgctcc tcgggggaca gcttgatctt ctccgcgtgg
     4081 ccgccgcggt ggaggttgga gcggaactcg cccttggcgc cctggcgctt catcgccgcg
     4141 atcacgcggt cgccgacgac gaaggcgcgg atgtcggcgc cctcggcctc tttgatgaac
     4201 tcctggacga ggatgttgac gtggaggccg cgaaacgcct cgatgacgga ggtggcggct
     4261 tgcttggtct ccgcgagcac gacgccgatg ccctgggtgc cctcgagcag cttgaccacc
     4321 aagggcgcgc cgccgacgat cgcgaccagg ccgtcgatgt ccttgatcga gtgggcgaag
     4381 ccggtgacgg gcaggccgat gcccttggcg gcgaggatct gcatgcagcg cagcttgtcg
     4441 cgcgagcgcg agatggcgat cgagctgttc ggcgagaaca cgcccatcat ctcgaactgg
     4501 cggacgacgg cggtgccata aaaggtgttg gaggcgccga tgcgggggat gacggcgtcg
     4561 aagccctcga gctgccggcc ctggtaggcg acctgcggct tgtgcgaggt gatgttcatg
     4621 tagcagcgca ggtagtcgat gacatagacc tcatgacctc gcttctgcgc cgcctccttg
     4681 aggcgggagg tcgagtacag cgacgccttg cgcgacaaga tgccgatctt catgtgggtt
     4741 tctgcttgcg gggccgggcg aagctggcac ggcgaccccc acatcggtag gagccgccag
     4801 gatcgacgat gaagcggcga cggagcgcct cacggccgag gagcatgcga aagccgagga
     4861 gcgcgcggtc ggtcagcgtg aactcggcct cgaaggtctg accgccgagc tcgacccgcg
     4921 tgcgcacgac cggccgcatc tcggcgacgc cgctcgagct gcggaccagc cgctcgtcga
     4981 ccaggtcggc ctcggcggtg atggcggcga cgtcgtcgcg ctggtgcggg tggacctgga
     5041 agcgcacgcg ctcgtgctcg acgcgctcga ccgcgaaggc gtgcagcgcg gaggtgcggg
     5101 cgccagtgtc gaccttggcc ttgagcatgg tgatgcccag gtcgggcaag cacacccact
     5161 cgcgccagcc gatcaccacg agggcgtcgc tctctccccg aagcatcgtg gccatctggt
     5221 cttggtcagg gcccggtggg gccgtggagc ttgctcagtg tgcgagcggg actgcgggtc
     5281 gccgaggctt ttaccttgcg ctcaaacgcc ggacagggtc aagccagggt ccgcgcgcca
     5341 gggcgcgcgg atgcgctggt tccgccgtgg cgtgcggatc gcgctgggcc ttgtccgcct
     5401 tgtccgcctt gtccgcatca tccgcattat ccgcatcatc atgcggccgc gaacgcaaaa
     5461 aagccgacca cgggctcttg cgagcgccgt ggtcggcgga ttcatgcgcg tgagcgtggc
     5521 tttagccgtt gctcagctgc gcgtactcgg tcttcggctt gcagcccggg tcgggcaggc
     5581 cgagctgacc gagcgccagg gcctgatcgt cgtgggtcgg gtgcaccggg atggcgatgc
     5641 cctcgacgat ctccacggcc gactcggcgt gggtgaagcg cggcgcccag agggtgtccg
     5701 aaacatcgat cccgttcggg ccggtgtggg tgaagtgcgg ggcccagagg gtcgtcggga
     5761 cgtcgaagtg gtcggtgtgg ccggtggccg cgggggccgt caggtcgtcc ggaccgacat
     5821 cggggtcgtt cgggggcagc ggcgcggcga tgtgcctggc cctcgggtcg accgagtgct
     5881 cgaggtcgtc gtcgtcctcg agggcggcga gctcgtcgag ctcctcctcg gacatcgcgt
     5941 cgatctcctc ggacgacagg cccagcgcct cgtactcctc gctgtcacag cccgggagga
     6001 aggcggcggt catggcgagc agggaaaggg agcgaagggt gttgcggatg gagagcgtca
     6061 tggcaaatgt ccttgcgaaa ggttgacgca ccctttgcca tcgtcgtgcc acgatcggag
     6121 ttgttgaaaa caaaggggat tttggtggtc gggaggcacc aggaccggaa aattggcaac
     6181 ggtcgtcgcc agatccagag gaggccatgg ctaaggtcgt ggccaggttt cgatcggcag
     6241 tttcgcggcg ttgaggaagc acgaggcgcc tgccacactg ccggcgccat gaccgaaggc
     6301 caccgatcca tccaaccccc gggctggccg cggccgcgcg ggtacagcaa cggcatggcc
     6361 gcgcgcggcg agctgctcgc ggtcgccggc atgatcggct gggacgccca ggagcgcctc
     6421 gtcggcgacg agttcgtgcc acagtttcgc caggcgctcg ccaacgtgtg cgacgtcgtc
     6481 cgcgcggccg gcggacagcc gcagcacatc gtgtcgctga cgctctacgt caccgacaag
     6541 caggcgtaca tcgcggggct gcgcgaggtc ggcgaggcgt accgggcgct gctcggcaag
     6601 cactacccgg cgatggcgct cgtgcaggtc gccgcgctgc tcgaggaccg cgcgaaggtc
     6661 gagatccagg ccctcgcggt gttgccgacc tcggaggccc cgtgaccgac acatcgcccg
     6721 acctgcgccc gctgccgctg ccggagccgc gttcgtttcg ttacgaggag cgctcgcgcg
     6781 tcggcgtgat cacgctgacc cgccccgacc gcttcaacgc gctgaccttc gaggtgtacc
     6841 gcgagctgac cgacctgttc ctggtggtcg accgccgcag cgaggagccc gagggcgccc
     6901 gcgcgctggt gttgcagggg gagggccggg cgttttgcag cggcggtgac gtcgaggaca
     6961 tcatcacgcg gctgttcgcg cgcgacaccg aggggctgct gcgcttcacc cggatgaccg
     7021 gcgagctgat ccacgcgatg cggcgctgta aactcccgat cgtcgccagc gtcaagcgcg
     7081 tggccgccgg ggccggcgca gtgatggccc tcgccagcga cctgcgcgtg atgggccaag
     7141 gcagcttctt ctcgttcctg ttcccgcagg tcggcctgtc gggcgccgac atgggcgcga
     7201 gctggctgct gccgcgcgtg gtcgggctcg gcaacgcctc ggagatcctg ctcatcggtg
     7261 accgcgtgcc cgccgaccgc tgcctgcaaa tggggctggc caatcgcgtt gtccccgacg
     7321 agcccgaccg cgacgcggtc gaccgcgcgg ccttcgagct cgcggcgcgg atcgccggcg
     7381 ggccgcactt cgcaaatcgc atgaccaaga caatgctgca gcaggagcag gacatgcact
     7441 ttaccgatgc gatcgaggcg
//
LOCUS       DASWNJ010000099         3858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_143234, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000099 DASWNJ010000000
VERSION     DASWNJ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143234"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VGB85_01855"
     CDS             complement(<1..735)
                     /locus_tag="VGB85_01855"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0132784.1"
                     /translation="MSLLRPTHSITATVLGAGTMGAQIAAHLANAGIRTFLLDMVPRD
                     TAADAPRAVRNSLALGALAQLTKAKPAAYTDPAHAARITPGNLEDDLERACAASDLVI
                     EAVVERLDIKQKLFTRVAAAAGPNTILTSNTSGLAIGAIADGLPEAARARLVGMHFFN
                     PPRYMHLLEVVPSKYSDEQVVAELSDFSDRVLGKGVVVCRDTPNFIGNRVGIAEMVLT
                     FRATAEGDYTIEEVDLLNGPLMGRPRT"
     gene            953..3364
                     /locus_tag="VGB85_01860"
     CDS             953..3364
                     /locus_tag="VGB85_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY0132785.1"
                     /translation="MSPNAAKAVVVAAAEPASVPTPAAPEHDPAALLASGQPDAALVE
                     LAKQTPALAGEPAWFVESALRGRAERLRGQPALASAALAPLAAARVVPPGLPRELILD
                     EYARSLMAEAASATAMPVKDADALRRKAAELWRKASKLEPVRNLAVMRVAHAEALAAI
                     EGEGGGRRVAATQAVKALTEVLRSYPQHPRAGALELARAQALQRAGKPRDAIAALRRV
                     AIDRTGTPEAEAAEAALTGLGKPPRWSPQEQIERATAARRIRRFEVSRSLLDDVLTDP
                     KTPAHLRPVAMRSRAFTASKQRDFAQYAADLETLYKASPSADLRDDLLKALDRAARFD
                     DAIALYMTQADKKGGTGKAALSGAIEQAIRGGRYAKAQALLERLPAKDRQRGDRAWQA
                     TWLKFRLGERDAAIAGFAALEKKLGGERATVARYFRGRLLLATPEDRPEGVKLLRAVI
                     AAGPLDYYGLMARQRLIDIGEEPGPAPVLSPMSSETATAPSAAEARALFATLASRHGA
                     AIPALRRGEFLYAAGWRDEAQRELRIAVDTLEAVAPVARGGWTPKHEDHVRGLSWLAT
                     WKQPKLAVPKEARKLVRTEGAVAELREGLRSLCWTLEEPHRMSRLMPGAAGSYKARWH
                     PRAFRGTVEREAGLRGVDPTQLWSLMYTESRFRRHVVSSVGARGAIQIMPNTGELLAA
                     RLGETLPDTDMLFDIDTNVHLAAYYLDELLDKFHGQAAMAYASYNGGPFNVERWLEAK
                     ADRAGGGQPLELDVFIAEIPLRETANYTRRVLEVQAAYNLLYRSELPRWQNTVDPVVE
                     GNIDF"
     gene            3497..>3858
                     /locus_tag="VGB85_01865"
     CDS             3497..>3858
                     /locus_tag="VGB85_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132786.1"
                     /translation="MNERLNQLGQAARRLNEGSDQLNTLISAIDKALARLMIGMDYVH
                     PRPLQETMSVGRDGKRVIELAFLSYLRIQSEFHLCIKTVKILESKVALASESPGNVIP
                     LLSAPRMLRHAAVDLLPEL"
BASE COUNT          582 a         1254 c         1499 g          523 t
ORIGIN      
        1 ggtgcgcggg cgacccatca acgggccgtt gagcaggtcg acctcctcga tcgtgtagtc
       61 gccctcggcg gtggcgcgga aggtcaggac catctcggcg atgccgacgc ggttgccgat
      121 gaagttcggg gtgtcacgac agacgaccac gcccttgccg agcacgcggt cggagaagtc
      181 ggagagctcg gcgacgacct gctcgtcgct gtacttcgag ggcacgacct cgagcaggtg
      241 catgtagcgc ggcgggttga agaagtgcat gccgacgagg cgggcacgcg cagcctcggg
      301 caggccgtcg gcgatcgcac cgatggcgag gccggaggtg ttggaggtga ggatcgtgtt
      361 cggccccgcg gccgcggcga cgcgcgtgaa cagcttttgc ttgatgtcga ggcgctcgac
      421 gacggcctcg atcacgaggt cactggcggc gcaggcccgc tccaggtcgt cctcgaggtt
      481 gcccggggtg atccgcgcgg cgtgggccgg gtcggtgtag gcggcgggct tggccttggt
      541 cagctgcgcg agcgcgccga gagcgaggct gttgcgcacc gccctggggg cgtccgcggc
      601 ggtgtcccgc gggaccatgt cgaggaggaa ggtccggatc ccggcgttgg cgaggtgggc
      661 ggcgatctgc gcccccatcg tgccagcgcc gagcaccgtg gccgtgatgg agtgggtggg
      721 acgaaggaga ctcacgcagc ggcagggtag aacaattagg gggaccccga tctcgagaaa
      781 atcttctcgg acgcccgtcc gtgctagcgt tcgcaagacg atgcgcgtgc gctccggacg
      841 gccgagcatg tgcgggtggc tggtcgccgc cggcctcctc gcgtgccgac ccgccgtgaa
      901 cgatgctgcg cagcgggtcc acccggccgt caatgtcatc catcatgagc ccgtgtcgcc
      961 gaatgcggcg aaggcggtgg ttgtggccgc ggcggagccg gcgagcgtac cgacaccggc
     1021 cgcgcccgag catgacccgg ccgcgctgct ggcgagcggg cagccggacg cggcgctcgt
     1081 cgaattggcg aagcagacac cggcgctcgc cggagagccg gcgtggttcg tcgagagcgc
     1141 gctgcgcggg cgggccgagc gattgcgagg tcagccggcg ctggcgagcg cggccctggc
     1201 tccgttggcc gcggcgcgcg tcgtgccgcc cggcttgccg cgcgagttga tcctcgacga
     1261 gtacgcgcgg agcctgatgg ccgaggccgc gagcgcgacg gcaatgccgg tcaaggatgc
     1321 cgacgcgctg cgccgcaagg cggccgagtt gtggcgcaag gcgagcaagc tcgaacccgt
     1381 gcgcaacctc gcggtgatgc gggtcgccca cgcggaggcc ctcgcggcga tcgaaggcga
     1441 gggcgggggc cgacgggtcg cggcgacgca ggcggtaaag gccctgacgg aggtgctgcg
     1501 cagctatccc cagcaccctc gcgcgggcgc gctcgagctg gcgcgcgcgc aggcgctgca
     1561 gcgggccggc aagccgcgcg acgcgatcgc tgcgctgcgg cgggtggcga tcgaccgcac
     1621 tggcacgccc gaggccgagg ccgccgaggc ggcgctgacg ggactcggta aaccaccgcg
     1681 ctggagcccg caggagcaga tcgagcgcgc gacggcggcg cggaggatcc gtcgcttcga
     1741 ggtgtcgcgg agcttgctcg acgacgtatt gacagatccg aagacgccgg cgcacctgcg
     1801 gccggtggcg atgcgctcgc gggcgttcac ggcgtcgaag cagcgggact tcgcgcagta
     1861 cgccgccgac ctcgagacac tttacaaggc gagcccctcc gccgacctgc gtgacgattt
     1921 attgaaggcg ctcgaccggg cggcgcgctt cgacgacgcg atcgcgctgt acatgacgca
     1981 ggccgacaag aagggcggca ccggcaaggc ggcgctgtcg ggggcgatcg agcaggcgat
     2041 ccgcgggggc aggtacgcga aggcgcaggc gctgctcgag cggctgccgg caaaggaccg
     2101 ccagcgcggc gaccgggcct ggcaggcgac ctggctcaag ttccggctcg gcgagcgcga
     2161 cgcggcgatc gccggcttcg cggcgcttga aaagaagctc ggcggcgagc gggcgacggt
     2221 cgcacggtac ttccgcgggc gcctgctgct cgccacgccg gaggaccggc ccgagggcgt
     2281 caagctgctg cgcgccgtga tcgcggccgg gccgctggat tactacgggt tgatggcgcg
     2341 ccagcgcctg atcgacatcg gcgaggagcc ggggcctgca cctgtccttt cgcccatgtc
     2401 ctcggagaca gccacggcgc cgtcggccgc cgaggcgcgg gcgctgttcg cgacgctggc
     2461 gagccgccac ggcgcggcga tcccggcgct gcggcgcggc gagtttttgt acgcggccgg
     2521 ctggcgcgac gaggcccagc gcgagctgag gatcgcggtc gacacgctcg aggcggtcgc
     2581 gccggtcgcc aggggcggct ggacgcccaa gcacgaggac cacgtgcgcg ggctgtcgtg
     2641 gctggcgacg tggaagcagc ccaagctggc ggtgccgaag gaggcgcgca agctggtgcg
     2701 cacggagggg gcggtcgccg agttgcgcga ggggctgcgc agcctgtgct ggacgctcga
     2761 ggagccgcat cgcatgtcgc ggctgatgcc gggcgcggcg gggtcgtaca aggcgcgctg
     2821 gcatccgcgg gcgttccggg ggacggtcga gcgcgaggcg gggctgcgtg gggtcgaccc
     2881 gacgcagctg tggtcgctga tgtacaccga atcgaggttt cgccggcacg tggtgtcgtc
     2941 ggtcggcgcg cgcggagcga tccagatcat gccgaacacg ggtgagctgc tggcggcgcg
     3001 gctcggcgag acgctgccgg acaccgacat gctgttcgac atcgacacca acgtgcacct
     3061 cgcggcctac tacctcgacg agctgctcga caagttccac gggcaagcgg cgatggccta
     3121 cgcgagctac aacggcggac ccttcaacgt ggagcgctgg ctcgaggcca aggcggaccg
     3181 cgccgggggt ggtcagccgc tcgagctgga cgtgttcatc gccgagatac cgctacgtga
     3241 gacggccaac tacacgcgtc gggtgctcga ggtgcaggcg gcctacaacc tgctgtatcg
     3301 cagtgagctg ccgcgctggc aaaataccgt cgacccggtg gtcgagggca acatcgactt
     3361 ttgaggcccg tgagcccggt gcgtaaaggc gccgtaaacg gggcttgtcg ggcggcctct
     3421 ggcagtctac cgtgcatgca cgccggggcc tgacagccgc ggcgcgccct gacgggctcc
     3481 ggaaggacac atggggatga acgaacgact caatcaattg ggccaggcgg cgcgccgcct
     3541 caacgagggc tccgaccagc tcaatacgct gatctcggcg atcgacaagg cgctcgcgcg
     3601 cctgatgatc gggatggact acgtgcatcc acggccgctg caggagacga tgagcgtcgg
     3661 ccgcgacggc aagcgggtca tcgagctcgc gttcctcagc tacctccgga tccagagcga
     3721 gtttcacctg tgcatcaaga cggtgaagat cctcgagtcc aaggtcgcgc tcgcgtccga
     3781 gagcccgggc aacgtcatcc ccctgctgtc cgcgccgcgg atgctgcggc acgcggcggt
     3841 cgacctgctg cccgagct
//
LOCUS       DASWNJ010000100         1553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_567084, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000100 DASWNJ010000000
VERSION     DASWNJ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567084"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..829)
                     /locus_tag="VGB85_01870"
     CDS             complement(206..829)
                     /locus_tag="VGB85_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132787.1"
                     /translation="MTEKRTATLTTYITDMHALVNHGLRTFDLIQQNLKGENHPEALA
                     MVADAHRTLTSQLTVLDARAQALGGKVTQPLKDAVTAVTGFAAGLIAAVRPEEASKAI
                     RDSYTYLSHVAIGYLMLYTTATGLGDSETASVAEQGYRDAARLVILIDKIMPTLVIQE
                     LREDGLPMTEVSDQVQTMVVQAWRREASGMGSGANSSGSGSNLGSAS"
     gene            complement(1181..>1553)
                     /locus_tag="VGB85_01875"
     CDS             complement(1181..>1553)
                     /locus_tag="VGB85_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0132788.1"
                     /translation="LRRLLEGELDIEVIGEAEDGRAAVRLAIELAPDVVVMDISMPLL
                     DGVEATRQIVEYNPSTRVVALSGHADQEAMAEVLRAGASAYLSKAAAYDELARAIRVA
                     MAEQRYLGASLGGLALPPVEP"
BASE COUNT          233 a          546 c          532 g          242 t
ORIGIN      
        1 tgttcgacgc gctcgagtcg cagctcaacg gcttcgatca ggacctcgag accctctatc
       61 gttatttcgg ccgcctgctc gatctaccgg ccacgacatc gaccccggcg ctgcccgccg
      121 ggatcgaggg accgcccagg ccgcgctgac gacccgtgga cgccgatctc ggccgccctg
      181 tacggcgcgg ccggatcgcg ctcgctcagc tcgccgagcc gaggttcgag ccggagcccg
      241 agctgttcgc gcccgagccc atgcccgagg cctcgcggcg ccacgcctgg acgaccatgg
      301 tctggacctg gtcggacacc tcggtcatcg gcaggccgtc ctcgcgcagc tcctggatca
      361 ccagcgtcgg catgatcttg tcgatgagga tcaccaggcg cgcggcgtcg cggtagccct
      421 gctccgcgac gctcgccgtc tcgctgtcgc cgaggccggt ggcggtcgtg tagagcatca
      481 ggtagccgat cgccacgtgg ctcaggtacg tgtaactgtc gcggatcgcc ttcgaggcct
      541 cctccggacg gaccgcggcg atcaggccgg cggcgaagcc cgtgacggcc gtgacggcgt
      601 ccttgagcgg ctgcgtcacc ttgccgccca gcgcctgcgc gcgtgcgtcg agcaccgtca
      661 gctggctggt cagcgtgcga tgagcgtccg ccaccatcgc cagcgcctcg gggtggttct
      721 cacccttgag gttctgctgg atgaggtcga aggtccgcag gccgtggttc accagcgcgt
      781 gcatgtcggt gatgtacgtg gtgagggtcg cagtgcgttt ctcggtcatg gttctctcct
      841 ggccgggaga catgcaggaa agcccggcag gggcaaccgc cgcgggcgcg acacggaccg
      901 caaagctccg gggtccggcg aggaacatgg tcacggctcc atgatggccg gcgcgcgacg
      961 gaccggcgga tctgcacata tcggggtgcg ccactcgatg cgcggcaggc gcgaaatgcg
     1021 tagctgccaa ctatcatcac aaaagcattt gcgcgatcgc gccacacttg cgcaggacag
     1081 gcagatttgg ccgcaattac gccggcacac gggtgggccg cgcgagcgac agcacggcgg
     1141 cccgacctcg cctcggacag gtacatctcc ccgcacggct tcagggttcg acaggaggga
     1201 gcgccaggcc cccgaggctg gcaccaaggt acctctgctc ggccatggcg acgcggatcg
     1261 cacgggcgag ctcgtcgtag gccgcggcct tgctcagata ggcggacgca ccggcgcgca
     1321 ggacctccgc catcgcctcc tggtccgcgt ggcccgagag cgcgacgacc cgcgtcgacg
     1381 ggttgtactc gacaatttgt cgcgtcgcct cgacgccgtc gagcagcggc atcgaaatgt
     1441 ccatgacgac gacgtctggc gcgagctcga tcgccagccg gaccgccgcg cgcccgtcct
     1501 cggcctcgcc gatgacctcg atgtcgagct cgccctcgag caggcggcgc agg
//
LOCUS       DASWNJ010000101         2288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_1808, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000101 DASWNJ010000000
VERSION     DASWNJ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1808"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..1290
                     /locus_tag="VGB85_01880"
     CDS             208..1290
                     /locus_tag="VGB85_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132789.1"
                     /translation="MPASAMLPGRLLALASLLLAACAGQLNPSVPIPKPGELGERPGV
                     WVAYNLGCPQGCDQIRRGDIITAIDGEPVTSGAEVDAADLARGTPVRLEVQSRKLGKI
                     DVTLVATPNEYMRPIEHAPPLWTVGAEALDRAPAWARLKAFGHATPAMRIYRLDDPRG
                     FRNGRELYGRHTLLVYWTPDNYRIQNQRRNVALLPRVYAQLQAHEAELRASGVDTYFM
                     FQGRYDPIVRNYTRSLIAVDPYGWAPNMIPIVANATDAGNANTLGLEHQASDLNELLF
                     NDGHVGPILLVIDARGIVRWHSRGWHQDDPVYSLEAVIDFAVRHLGDTPPAEEVPAPE
                     PGATPAAPDAPVVEPAPAAPLVAPPM"
     gene            complement(1320..>2288)
                     /locus_tag="VGB85_01885"
     CDS             complement(1320..>2288)
                     /locus_tag="VGB85_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132790.1"
                     /translation="QIEITTFRGEGVYADGRRPSEVRFLRALPDDLARRDFTINAFAW
                     NPITRVFTDCFDGLADLAHGVLRAVGDPAARFQEDGLRAMRAVRLCATLEMRLDPATA
                     DAIGGALPILDLVSRERVHVELFKLLAARRPSLGLEPMAQTGIWPRVLAPISAAEVHA
                     AISAVELMRPDPVVRLARLLWPLRDDRRRIEAIVDGLKPSRDERVRVLALTDPAIAAL
                     ARERDPLAIRRVAAALGRRHLADATDLLARDWAQLVTIEEACRGAALAVGELAIGGKD
                     LVAGGLAVPGPGLGKTLAALLDWVLEAPARNTAPELLARARALASQ"
BASE COUNT          320 a          913 c          772 g          283 t
ORIGIN      
        1 gcgaccgcag cgagcgcgcc gaacgggcca gcgaccgcga ggacgagctg tccccgggga
       61 caggtcgcct gatcagcgtc gagctcagcc cgctgcgcct cgtcggcgcg ccccggggct
      121 acctgaccct cgacgcccgc atcgtccagc gcgcccacct cgagggcatg tagcggggcg
      181 catgtcgctc gacacgaccg gcgcgtgatg ccagcctctg ccatgctccc gggccgactg
      241 ctcgccctcg cgtcgctgct gctcgccgcc tgcgccggcc agctcaaccc ctcggtgccg
      301 atcccgaagc ccggcgagct cggcgagcga ccgggcgtat gggtcgccta caacctcggc
      361 tgcccgcagg gctgcgacca gatccgtcgc ggcgacatca tcaccgcgat cgacggcgag
      421 ccggtcacca gcggggccga ggtcgacgcc gccgacctcg cgcgcggcac cccggtccgc
      481 ctcgaggtcc agtcgcgcaa gctcggcaag atcgacgtca ccctggtggc gaccccgaac
      541 gagtacatgc ggccgatcga gcacgcgccc ccgctgtgga cggtcggcgc cgaggccctc
      601 gaccgcgccc ccgcgtgggc ccgcctcaag gccttcggcc acgcgacccc ggcgatgcgg
      661 atctatcgcc tcgacgaccc ccgcggcttt cgcaacggcc gcgagctgta cggccgccac
      721 accctgctcg tctactggac cccggacaac taccggatcc agaaccagcg ccgcaacgtc
      781 gccctcttgc cccgcgtcta cgcccagctg caagcccacg aggccgagct gcgtgcttcg
      841 ggcgtcgaca cctacttcat gttccagggc cgctacgacc cgatcgtccg caattacacg
      901 cgcagcctga tcgccgtcga cccgtatggc tgggccccga acatgatccc gatcgtcgcc
      961 aacgccaccg acgccggcaa cgccaacacc ctcggcctcg aacaccaggc ctccgacctc
     1021 aacgagctgc tgttcaacga cggccacgtc ggaccgatcc tgctcgtcat cgacgcccgc
     1081 gggatcgtcc gctggcattc gcgcggctgg catcaggacg accccgtcta cagcctcgag
     1141 gccgtgatcg acttcgcggt ccgccacctc ggcgatacac cgcccgcaga ggaggtgccc
     1201 gcgcccgagc cgggagcgac acccgcggcg cccgatgctc ccgtggtcga gcctgcgccg
     1261 gcggctccgc tcgtggctcc gccgatgtga cgcgggcaca cggacggcct ccggacccac
     1321 tactggctgg cgagcgcccg cgcccgcgcg agcagctccg gggcagtgtt acgcgcaggc
     1381 gcctctagca cccagtcgag cagcgccgcg agcgtttttc cgaggcccgg gccaggcacg
     1441 gcgaggccgc cggcgacgag gtccttgccg ccgatcgcca gctcaccgac cgcgagcgcg
     1501 gccccgcggc aggcctcctc gatcgtgacg agctgggccc agtcgcgcgc gagcaggtcg
     1561 gtcgcgtcgg cgaggtgccg gcgcccgagc gccgcggcga ccctgcggat cgccagcggg
     1621 tcgcgctcgc gtgccagcgc cgcgatcgcg gggtcggtca gcgcgagcac gcggacccgc
     1681 tcgtcgcggc tcggcttgag cccgtcgacg atcgcctcga tccggcgccg gtcgtcgcgc
     1741 agcggccaca gcagccgcgc gaggcggacc accgggtcgg ggcgcatcag ctcgacggcc
     1801 gagatcgccg cgtggacctc cgccgccgag atcggcgcga gcacccgcgg ccagatcccc
     1861 gtctgcgcca ttggttcgag gcccagcgag ggccgccgcg cggcgagcag cttgaacagc
     1921 tcgacgtgca cgcgctcgcg gctgacgagg tcgaggatcg gcagggcccc gccgatcgcg
     1981 tcggccgtcg cggggtcgag gcgcatctcg agggtcgcgc acagccgcac cgctcgcatc
     2041 gcccgcagcc cgtcctcctg gaaccgcgcc gccgggtcgc cgaccgcccg cagcaccccg
     2101 tgtgcgaggt cggcgaggcc gtcgaagcag tcggtgaaga ccctcgtgat cgggttccac
     2161 gcgaacgcgt tgatcgtgaa gtcgcggcgc gccaggtcgt ccggcaaggc gcgcaggaac
     2221 cggacctcgc tcggccggcg tccgtccgcg tacacgccct cgccgcgaaa ggtcgtgatc
     2281 tcgatctg
//
LOCUS       DASWNJ010000102         3781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_1837, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000102 DASWNJ010000000
VERSION     DASWNJ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1837"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..428)
                     /locus_tag="VGB85_01890"
     CDS             complement(30..428)
                     /locus_tag="VGB85_01890"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0132791.1"
                     /translation="MFSFHHVGVVTADAERAAEFYADLGYTRSERFDDPLQRVGIILL
                     EKAGSPMIEIVTPADPGSPAAGWLKRIKAGPYHTCYQVPDIAEATEFLRERGAMPLGE
                     PLPAIAFGGRRVVFLFGAVSGLVELVEENP"
     gene            complement(477..1550)
                     /locus_tag="VGB85_01895"
     CDS             complement(477..1550)
                     /locus_tag="VGB85_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132792.1"
                     /translation="MHLRSLLRQLLITAGFFVAWLWLLGQVIRVFYPEDDEAAVDTYR
                     SASPLVSDAPSIVLYEFDRLRRPADKRMIVLGSSTGMLNLRADHLRPDFPGWDVHNMA
                     IPTSNMDQIYQVLDMIIAVSDEQTLDNSVFILPLYYVMFSDRLYGGKDTPLSVEMLRY
                     GLYRSQEGDTPTPLLGYRFASVTHGLLRVFLGIDKVSKAVAGARSDLDAGKPINWGRF
                     RPTIRQPYKYAKKPPDAKFQRSENARRIKQIGLPDSMEHGQFERIEEIADRVLSVGAR
                     LIIVDGPVPTWNRGVHFKYYREHKQSFVAALARKPGVAYFDFTDLLPDDLFGDSAHTM
                     IKGSEKFEDAVAQAIQGLRDPIR"
     gene            complement(1564..3006)
                     /locus_tag="VGB85_01900"
     CDS             complement(1564..3006)
                     /locus_tag="VGB85_01900"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEY0132793.1"
                     /translation="MGLLSLQFLLLVSIVIVLYRVVPWQAGRVALLTAANVAFVASFA
                     DEPAALLPFAAYLLQGWICVQALRVYQSRAALGVAVAWMVCVFIVLKQYSFIQPLLEP
                     AHVYVTIGFSYVLFRVLHLMIDTATGEKIEGLSPLRYFNYTCMFLSFVSGPIQRYEDY
                     SREETRRDQPPLDGPLFFAVLTRIIIGLIKLVFLAPAATTVLEGCLRSMAGAGGLTAG
                     VPVLVQLAAANLAFVLFLYWNFSGSMDVVIGAGRAMGFTLPENFNRPLGAPNLIDFWT
                     RWHMTLSNWFRVYLFNPTVTFLTRMNTNIRFAPYYGVVGFFVTFFVMGAWHGTTAVII
                     LHGLILGAGVSLNKFYQIKMRGWLGKKRHLKLNDNYVYTKLCNGLTITVLGVALACFW
                     LEGSDLHRLLRTIGLTGLLAAFGCSVLLTAIAATFIDGLKWFTAKTRIDDALAWPWSR
                     QALVALGLLMLVLYAALTKSDAPAFVYATF"
     gene            complement(3013..3261)
                     /locus_tag="VGB85_01905"
     CDS             complement(3013..3261)
                     /locus_tag="VGB85_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY0132794.1"
                     /translation="MSKQEAIKQRLLGIFRQVFDDDELEISSTTTAADIEDWDSLSHI
                     TLIVACEKEFGLKLSPKEIGDLKNVGEMLVLLEQRATR"
     gene            complement(3394..>3781)
                     /locus_tag="VGB85_01910"
     CDS             complement(3394..>3781)
                     /locus_tag="VGB85_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132795.1"
                     /translation="PAATALRAGDLAAKLGERDAAIAWFERVLATPEEVSDRPRSIAR
                     ATLRLATLLAERKADVPRACALLAAADAAPPAEDTDRNADADRDVDVYAELGALRTAL
                     CEGRPRPPSPPRKGAPSRPTGGGESP"
BASE COUNT          644 a         1249 c         1313 g          575 t
ORIGIN      
        1 ggccgcgacg atgcgctcgg ctgctgcgtt catgggttct cctcgaccag ctcgaccagc
       61 ccggacaccg cgccgaacag gaacaccacc cgccggccgc cgaacgcgat cgccggcagc
      121 ggctcgccca gcggcatcgc cccgcgctcg cgcaggaact cggtcgcctc cgcgatgtcg
      181 ggcacctgat agcaagtatg atagggcccg gctttgatcc gcttgagcca gcccgccgcg
      241 gggctcccgg gatcggccgg cgtgacgatc tcgatcatcg ggctcccggc cttctcgagc
      301 aagatgatgc cgacgcgctg cagcggatcg tcgaagcgct cggacctcgt gtaaccgagg
      361 tcggcgtaga actccgcggc acgctcggcg tccgcggtca cgaccccgac gtggtggaac
      421 gaaaacacgg gcgcacccta gcaggagcgc cctgcttcgt cgagaccggg ctgcggctac
      481 cggatcggat cgcgcagccc ctggatcgcc tgggcgacgg cgtcctcgaa cttctcggag
      541 cccttgatca tcgtgtgcgc cgagtcgccg aacaggtcgt cggggagcag gtcggtgaag
      601 tcgaagtagg cgacgccggg cttgcgcgcg agggcggcga cgaagctctg cttgtgctcg
      661 cggtagtact tgaagtgcac gccgcggttc caggtcggca ccgggccgtc gacgatgatc
      721 aggcgcgcgc cgaccgagag cacgcggtcg gcgatctcct cgatgcgctc gaactggccg
      781 tgctccatgc tgtcgggcag gccgatctgt ttaatacgcc gcgcgttctc cgagcgctgg
      841 aacttcgcgt ccggcggctt cttggcgtac ttgtagggct ggcggatcgt cgggcggaac
      901 ctgccccagt tgatcggctt gccggcgtcg aggtcggacc tggcccccgc gaccgccttc
      961 gacaccttgt cgatgccgag gaacacccgc aagagcccgt gcgtcaccga agcgaagcgg
     1021 tagccgagca gcggcgtcgg cgtgtcgccc tcctgcgagc gatacaggcc ataacgcagc
     1081 atctcgacgc tgagcggcgt gtccttgccg ccgtacagcc ggtccgagaa catcacataa
     1141 tacagcggca ggatgaacac cgagttgtcg agcgtctgct cgtcgctgac ggcgatgatc
     1201 atgtcgagga cctgatagat ctggtccatg ttcgacgtcg ggatcgccat gttgtggacg
     1261 tcccagccgg ggaagtccgg gcgcaggtgg tcggcgcgca ggttgagcat gccggtcgac
     1321 gagccgagca cgatcatgcg cttgtcggcc gggcggcgca ggcggtcgaa ctcgtagagc
     1381 acgatgctcg gcgcgtcgct gaccagcggc gaggcgctgc gataggtgtc gaccgcggcc
     1441 tcgtcatcct ccgggtagaa cacgcggatc acctgcccga gcagccacaa ccaggcgacg
     1501 aagaagccgg cggtgatcag gagctgacgc agcagtgagc ggaggtgcat gtctctcccc
     1561 tttttaaaat gtcgcgtaga cgaaggccgg cgcgtcgctc ttggtcagcg cggcgtagag
     1621 caccagcatc agcagcccga gggccaccag cgcctgacgc gaccacggcc acgcgagcgc
     1681 gtcatcgatg cgcgtcttcg cggtgaacca tttcaggccg tcgatgaagg tcgccgcgat
     1741 cgccgtcaag aggacgctgc acccgaacgc ggcgagcagg cccgtcaggc cgatcgtgcg
     1801 cagcagccgg tggaggtcgc tgccctcgag ccagaagcac gcgagcgcca cgccgagcac
     1861 ggtgatcgtc aggccgttgc acagcttggt atatacgtag ttgtcgttga gcttcaggtg
     1921 gcgcttcttg ccgagccagc cgcgcatctt gatctgatag aacttgttga ggctgacgcc
     1981 ggcgccgagg atcaggccgt gcaggatgat caccgcggtc gtcccgtgcc acgcgcccat
     2041 cacgaagaag gtgacgaaga agccgaccac cccgtaatag ggcgcgaacc ggatgttcgt
     2101 gttcatccgg gtcaggaacg tgaccgtcgg gttgaacaga tagacgcgga accagttgga
     2161 cagcgtcatg tgccagcgcg tccagaagtc gatcaggttc ggcgcgccga gcgggcggtt
     2221 gaagttctcc ggcagcgtga agcccatcgc ccggcccgcg ccgatcacga cgtccatcga
     2281 gcccgagaag ttccagtaca aaaacagcac gaacgccagg ttggccgcgg cgagctgcac
     2341 gagcaccggc acgcccgccg tcaggccccc ggcgccggcc atcgagcgca ggcagccctc
     2401 gagcaccgtc gtcgccgccg gcgcgaggaa caccagcttg atcaggccga tgatgatccg
     2461 cgtcagcacc gcgaagaaca gcggcccgtc gagcggtggt tgatcgcgcc gcgtctcctc
     2521 gcgcgagtaa tcctcgtagc gctggatcgg gcccgagacg aacgacagga acatgcacgt
     2581 ataattaaaa taccgcagcg ggctgaggcc ctcgatcttc tcgccggtgg cggtgtcgat
     2641 catcaggtgc agcacacgga acagcacgta cgagaagccg atcgtgacgt agacgtgcgc
     2701 cggctcgagc agcggctgga tgaagctgta ctgcttgagg acgatgaaga cgcagaccat
     2761 ccacgcgacc gcgacgccga gcgcggcccg gctctggtac acgcgcagcg cctgcacgca
     2821 gatccagccc tgcagcaggt acgcggcgaa cggcagcagc gccgccggct cgtcggcaaa
     2881 ggacgcgacg aaggcgacgt tggcggcggt cagcagggcg acgcgccctg cttgccaggg
     2941 gacgacgcga tataaaacga tgacgatgct gaccagcagc agaaactgga gcgacaggag
     3001 gcccatcggc ggctaccgcg tggcgcgctg ctcgagcagg acgagcatct cccccacgtt
     3061 cttgaggtcg ccgatctcct tgggagacag cttgagcccg aactccttct cgcacgcgac
     3121 gatcagcgtg atgtgcgaca gcgagtccca gtcctcgatg tccgcggccg tcgtggtgct
     3181 ggagatctcg agctcgtcgt cgtcgaagac ctgacgaaag atgccgagga gccgctgctt
     3241 gatcgcttct tgcttgctca tgatgttaag gcggctacct tctagccgct ggcccgcgag
     3301 gtgtcaaatg ccgccggggc cgcgaaaacc cacgggggag ccgccacggc gcttcgccgc
     3361 gcttcgtgct ccgggctcac gcgtctgcac gtgtcagggt gactcgccgc cgccggtcgg
     3421 cctcgacggc gcgcccttcc tcggggggct cggggggcgc gggcgaccct cgcacagggc
     3481 ggtccggagc gcgccgagct cggcgtacac gtcgacgtcg cggtcggcgt cggcgttgcg
     3541 gtcggtgtcc tcggcggggg gcgccgcgtc ggccgcggcc aggagggcgc acgcgcgggg
     3601 gacgtcggcc ttgcgctccg cgagcagcgt cgccagacgc agggtggccc gggcgatcga
     3661 ccgcgggcga tcgctgactt cctcgggcgt cgcgagcacg cgctcgaacc atgcgatcgc
     3721 cgcgtcgcgc tcgccgagct tcgcggcgag gtctccggcg cgcagggccg tcgcggccgg
     3781 g
//
LOCUS       DASWNJ010000103         2049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_638230, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000103 DASWNJ010000000
VERSION     DASWNJ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638230"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VGB85_01915"
     CDS             complement(<1..669)
                     /locus_tag="VGB85_01915"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HEY0132796.1"
                     /translation="MLRARLSVSFALSSLFVLACTGGKPDSAPPTGDEAGAVAGGTSS
                     TFKFENPGGMWMPRQMALHAETLKKLGLAFDPGALTDPTAFPLGAVISLGGCTASFVS
                     PDGLIITNHHCVTRYLQHNSTPEQNLLKNGYLAATRADERSGGPTARVWVTTAFTDVT
                     DKVLGGLESVTDDKQRYTQIEDRRKQIVNACEAGRPEIACTVSSFFEGAQFFQIEQAE
                     IRDLR"
     gene            900..1955
                     /locus_tag="VGB85_01920"
     CDS             900..1955
                     /locus_tag="VGB85_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY0132797.1"
                     /translation="MPKPRTSLLVGNPTAQSGKAAKAIEAAMAGLTEARLQPEFFSTL
                     PDGATVAVLAQRLERGDIARVIYLGGDGTFAETAKGIILAREQYGIDVPMGMLPMGTA
                     NDQGRSFGVRSGIKALPNNIKVIAAGIEQWLDVGKVDAFDAAGELLRTDLWFDSWGMG
                     LSAQILAKRNRERKIVARIPVIRKIYRDKLVYIRAGLSSWLKNMFKSLIGRARFAAEV
                     RIDEQTTRYEALNDIVIKGTMLYGGDWIFAPDSKPDDGKFEVILVKSSADWGAAAIGA
                     HKRNPVTGDDLAVLGLSPRVIPSGRVIEIRVTRPPGMMPLPAQIDGEEFLPADHYRIE
                     NLYHYLRILVPEDAHWV"
BASE COUNT          318 a          676 c          746 g          309 t
ORIGIN      
        1 gcgcaggtca cggatctcgg cctgctcgat ctggaagaac tgcgcgccct cgaagaagct
       61 cgagacagtg catgcgatct ccgggcggcc agcctcgcag gcgttgacga tctgcttgcg
      121 gcggtcctcg atctgcgtgt agcgctgctt gtcgtcggtg acgctctcga ggccgccgag
      181 caccttgtcg gtgacgtcgg tgaaggcggt cgtcacccac acgcgcgcgg tcggaccgcc
      241 ggagcgctcg tcggcccgtg tcgcggcgag gtagccgttc ttgagcaggt tctgctccgg
      301 ggtgctgttg tgctggagat agcgggtgac gcagtgatgg ttggtgatga tcaggccgtc
      361 gggcgacacg aacgaggccg tgcagccgcc gaggctgatc accgcgccga gcgggaaggc
      421 ggtcgggtcg gtcagcgcgc cggggtcgaa ggcgaggccg agcttcttga gcgtctcggc
      481 gtgcagcgcc atctgccgcg gcatccacat gccgcccggg ttctcgaact tgaaggtcga
      541 cgaggtgcca cccgcgacgg cccccgcctc gtcgccggtc ggcggcgccg agtcgggctt
      601 gccgccggta caggcgagca cgaacagcga agagagggcg aaagagacgg acaggcgcgc
      661 gcggagcatg gcttgcaggg gtatcagatc tgcgcgagac ccgggcaggg ccgcgcccgt
      721 gagtcgccgc ccgggccggc gcgaaacccg gtccccgcgg gtcgggtctt gccccctgcc
      781 ccggcgctcg cccgatcctg ccgttggcga gcgcgcaggg ccgtgagcaa ggtccccggg
      841 gcgtcgcgcc gccgacggac gctcgcgttt cgtcgcgggc gtgtaggcta cgggcccccg
      901 tgcccaagcc tcgaacatcg ctgctcgtcg gcaacccgac cgcccagtcc ggcaaggccg
      961 cgaaggcgat cgaggcggcg atggccgggc tcacggaggc gcggctgcag ccggagttct
     1021 tctcgacgct gcccgacggt gcgacggtgg cggtgctcgc ccagcgtctc gagcgcggcg
     1081 acatcgcgcg cgtgatctac ctcggcggcg acgggacgtt cgctgagacg gccaagggca
     1141 tcatcctggc gcgcgagcaa tacggcatcg acgtgccgat gggcatgctg ccgatgggga
     1201 ccgcgaacga tcagggtcgt tcgttcgggg tgcgctcggg gatcaaggcg ctgccgaaca
     1261 acatcaaggt gatcgcggcg ggcatcgagc agtggctcga cgtcggcaag gtcgacgcct
     1321 tcgacgccgc cggcgagctg ctgcgcacgg acttatggtt cgacagctgg ggcatgggcc
     1381 tgtcggcgca gatcctggcc aagcgcaacc gcgagcgcaa gatcgtggcc cggatcccgg
     1441 tgatccggaa gatctaccgc gacaagctcg tgtacatccg cgcggggctg tcgagctggc
     1501 tcaagaacat gttcaagtcg ctgatcggtc gggcccgctt cgccgcggag gtccgcatcg
     1561 acgagcagac gacccgctac gaggcgctca acgacatcgt catcaagggc acgatgctgt
     1621 acggcggcga ctggatcttc gcgcccgaca gcaagcccga cgacggcaag ttcgaggtga
     1681 tcctggtcaa gagcagcgcc gactggggcg ccgcggcgat cggcgcgcac aagcgcaacc
     1741 cggtgaccgg cgacgacctc gcggtgctcg gcctgtcgcc gcgcgtgatc ccgagcgggc
     1801 gcgtgatcga gatccgcgtc acgcggccgc cggggatgat gccgctgccg gcccagatcg
     1861 acggcgagga gttcctgccg gccgaccact atcgcatcga aaatctctat cattacctgc
     1921 gcatcctcgt ccccgaggac gcccactggg tgtgacatgg cgatccatcg tgccaccatc
     1981 ctcatcatct tgtgtgcgat cggcctggtg acgggctgcg gcgaggacct gcgcaacagc
     2041 atcggcgag
//
LOCUS       DASWNJ010000104         2573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_284483, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000104 DASWNJ010000000
VERSION     DASWNJ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284483"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VGB85_01925"
     CDS             <1..226
                     /locus_tag="VGB85_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132798.1"
                     /translation="ALAGGPATAARLAVALVAAAGARARWPEPAIAALRALRSHPDLR
                     TRHAAHQVFTRPEALSADEDDEEEEEDDDE"
     gene            complement(238..1149)
                     /locus_tag="VGB85_01930"
     CDS             complement(238..1149)
                     /locus_tag="VGB85_01930"
                     /inference="COORDINATES: protein motif:HMM:NF039450.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADYC domain-containing protein"
                     /protein_id="HEY0132799.1"
                     /translation="MTATLTSGCDPDTLADDADGVVHFRPGGGVWLNTSSIGAQPFSE
                     FDTSGIVHDGVRFTGLLLKRADNTWLAATPEVTDGNLKGKVGKTAYVGVDLVGSRWKF
                     NMVDGAVETPVEIWIESYTQVSPKEARYTFKTLDSNGVAQPICDADLNGSVAVTPIRD
                     ITVDSATGDMVSRTKTVYLACTSGALGKAIAWGYRPWERPLQEFEAATRMVRADYCYD
                     GMSWTETGISLQVHDKYGVNTFLDATDPNEAVWLATGAACIGTPRNPTYGPATVTCNG
                     QALPSCPANVGMSTYAGSLFWTKRNVP"
     gene            complement(1550..>2573)
                     /locus_tag="VGB85_01935"
     CDS             complement(1550..>2573)
                     /locus_tag="VGB85_01935"
                     /inference="COORDINATES: protein motif:HMM:NF039450.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ADYC domain-containing protein"
                     /protein_id="HEY0132800.1"
                     /translation="GDLPTMNTHRFVSILSAALITVGFTSGCDPESVEADGTQDEGVD
                     PRIGGGVWLNTSSIGSKAFSEFDLTGQLHDGVRLTGALLKRPNNQWATISTGEVVDGQ
                     LRAKVGNTTYTGAALIGSRWKLQIPDYDYDHDDDDDDDDDDNGVRNVEIWVSAYTQIA
                     PNEGLYTFQTLDEQGQPTYICDADSSGSHAAIPVRDITVNRVTGHISARPKTAYLACT
                     SGAIGKAITWGYKPWQRSLTDFEVATRMVRADYCFDGNSWTQTGTALQIRDAYDINDF
                     AFATEPTEVVWTRTGVACLTQPRNITYAAPQITCGGQALPICPVDVSLATYPSALFWT
                     KVDTLL"
BASE COUNT          366 a          834 c          937 g          436 t
ORIGIN      
        1 cgcgctcgcc gggggtccag cgaccgcggc gcgcctggcc gtcgcgctcg tcgcggccgc
       61 gggcgcgcgc gcccgctggc cggagcctgc gatcgccgcg ctgcgtgccc tccgatccca
      121 ccccgacctg cgcacgcgtc acgcggcgca tcaggtgttc acgcgccccg aggccctgtc
      181 ggcggacgag gacgatgaag aggaggagga ggacgacgac gagtgacgct cacgcggtca
      241 cggcacgttg cgcttggtcc agaacagcga gccggcgtag gtcgacatgc cgacgttggc
      301 cgggcagctc ggcagggcct ggccgttgca ggtcaccgtc gctggcccgt acgtggggtt
      361 gcgcggggtg ccgatgcacg ccgcgccggt cgccagccac acggcctcgt tcgggtcggt
      421 ggcgtcgagg aaggtgttga cgccgtactt gtcgtggacc tgcagcgaga tgccggtctc
      481 ggtccaggac atgccatcgt aacagtagtc ggcgcggacc atccgggtgg cggcctcgaa
      541 ctcctggagc ggccgctccc acgggcgata accccacgcg atcgccttgc cgagcgcgcc
      601 ggaggtgcac gcgaggtaga ccgtcttcgt acgcgacacc atgtcgccgg tggcagagtc
      661 gacggtgatg tcccggatcg gggtcacggc gacgctcccg ttcagatccg cgtcacagat
      721 cggctgcgcg acgccgttcg agtcgagggt cttgaaggtg taacgcgcct ccttgggcga
      781 gacctgcgtg tacgactcga tccagatctc gaccggggtc tcgaccgcgc cgtcgaccat
      841 gttgaacttc cagcgcgaac cgaccaggtc gacgcccacg taggcagtct tgccgacctt
      901 gcccttgagg ttgccgtcgg tgacctccgg ggtcgcggcg agccacgtgt tgtccgcgcg
      961 cttgagcagc aggccggtga agcgcacacc atcgtgcacg atcccggagg tgtcgaactc
     1021 cgagaacggc tgggcgccga tcgagctggt attgagccag acgccgccgc cgggacggaa
     1081 gtggacgacg ccatcggcgt cgtcggcgag cgtatcgggg tcacacccgg aggtgagggt
     1141 cgctgtcaac agcgaggagg cgaggacaag gcatcggtgg gcgtacatcg gcgggtctcc
     1201 gtggcgctcc gagcatttcg actgcggcca cgttcctctg tgacacgtcc ctcgccgctt
     1261 gcagcgctcg agaagttgtc caccccgagc gtcccggacg ttcgtctcga gcttgcgttc
     1321 ctgcgccgaa gttcgtcaag atcggcagca atatcagccg tacggtgatg taggcggagc
     1381 gaagcgcctg ccgctcctgc accgcgaacg cgccccgcgg gcagctcgtc aggtgcctcg
     1441 tggagggggc ccgttttcac tgcgcagcac gcggtcgatc gaccgccgtc tcgacgacaa
     1501 aaggccccgc atccacgagg gacacggggc cgggcgcggc gaaacgtgct cagagcagcg
     1561 tgtcgacctt ggtccagaac agcgcgctcg ggtaggtcgc caggctcacg tccaccgggc
     1621 agatcggcag cgcctggccg ccgcaggtga tctggggcgc ggcgtaggta atgttgcgcg
     1681 gctgggtcag gcaggcgacg ccagtcctgg tccacacgac ctcggtcggc tcggtcgcga
     1741 acgcgaagtc gttgatgtcg tacgcgtcgc ggatctgcag cgcggtgccg gtctgcgtcc
     1801 agctgttgcc gtcgaagcag tagtcggcgc ggaccatgcg ggtcgcgacc tcgaagtcgg
     1861 tcaggctgcg ctgccagggc ttgtagcccc acgtgatcgc cttgccgatc gcgccggagg
     1921 tacacgcgag gtacgccgtc ttcgggcggg cgctgatgtg gccggtgacg cggttcacgg
     1981 tgatgtcgcg taccgggatc gcggcgtggc tgccgctgct gtcggcgtcg cagatatagg
     2041 tcggctggcc ctgctcgtcg agggtctgga aggtgtagag cccctcgttc ggcgcgatct
     2101 gggtgtacgc cgagacccag atctcgacgt tgcgcacgcc gttgtcgtcg tcgtcgtcgt
     2161 cgtcgtcgtc gtcgtggtcg tagtcgtagt cggggatctg gagcttccag cgcgagccga
     2221 tcagcgcggc gccggtgtag gtggtgttgc cgaccttggc ccgcagctgg ccgtcgacga
     2281 cctcgccggt gctgatcgtc gcccattgat tgttcgggcg cttgagcagc gcgccggtca
     2341 ggcgcacgcc gtcgtgcagc tggccggtca ggtcgaactc ggagaacgcc ttgctgccga
     2401 tcgagctggt gttgagccac acgccgccgc cgatgcgcgg gtcgacccct tcgtcctgcg
     2461 ttccgtcggc ctcgacgctc tcggggtcgc agccggatgt gaagccgacg gtgatcagcg
     2521 cggcggacag gatggacacg aaacggtggg tgttcatggt gggtaggtct ccg
//
LOCUS       DASWNJ010000105         9274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_672814, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000105 DASWNJ010000000
VERSION     DASWNJ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 9274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9274
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672814"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VGB85_01940"
     CDS             <1..207
                     /locus_tag="VGB85_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HEY0132801.1"
                     /translation="SIALLAAGRARAAMEGRDFVIPDDIKSLAPSVLRHRLILHPDAE
                     FEGTSADDCIQTVLTEARVPRTAA"
     gene            223..1536
                     /locus_tag="VGB85_01945"
     CDS             223..1536
                     /locus_tag="VGB85_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY0132802.1"
                     /translation="MIPSRLFVLLMLVPLALAIAAIADRTLLWPMLGADGFIAMVAAM
                     DALLARRPLVEVRRTSREVFSIGRSNVVTLELRSKARRRLTVRVTDDLFEHASAPELP
                     LELDLRGGSRAEASYRVVPHRRGTYALGDHTIRYRSPLGLWIRQITQRASQPVRIFPD
                     VQAVRDYELLARQSRDAAARASRRRGGASEFEALREYQRDDEYRKIDWKATARRHKLI
                     ARTYQLERDQSVVFMLDAGRLMTAESDALSLFDHALNATLMLTYVASRGGDQVGLMTF
                     ADRVLTYLPPGPGKSASQRLIRSLFAVHPELVETDYEGAFTSLGPRLRKRSLVVLFTQ
                     ILDHNAAQQLLRLTRGLLPRHLPLVVLLRDPAVEALVQIEPDQFALGDHRPFITAAAA
                     EVLSWREKLIRDLRRAGALVLDVLPRELSPSLINRYLEVKARQML"
     gene            1658..2596
                     /locus_tag="VGB85_01950"
     CDS             1658..2596
                     /locus_tag="VGB85_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triacylglycerol lipase"
                     /protein_id="HEY0132803.1"
                     /translation="MQFPKRWLPASLAIAVLGTLCIPGTASAGDYTKTKHPIVLAHGM
                     SGFDELFGIYEYWQSIPQALIKDGAKVFVTEVPQFNSTEARGEALLAQVEEILAITGA
                     AKVNLIGHSHGGLDVRYVASVAPGLVASVTTVGSPHHGAELADFLRNNVKGGGFTEVV
                     LGLFANSLGKVLALLSGANNQQDAIAGLGALTTEGLSTFNAAHPAGLPAKWCGEGPAV
                     VGGIHFYSWTGDRALTNILDPSDALLGLTSIVYDEDNDGLVGKCSAHLGKVIRDDYRL
                     NHLDEVNQAFGLVHLFGPDPKSLFRVHANRLKNEGM"
     gene            2599..3426
                     /locus_tag="VGB85_01955"
     CDS             2599..3426
                     /locus_tag="VGB85_01955"
                     /inference="COORDINATES: protein motif:HMM:NF015252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase secretion chaperone"
                     /protein_id="HEY0132804.1"
                     /translation="MRLHHFVLVSLTLLAACYSGAEAQPTQPRSLAGTEIDGELRTER
                     GMFVFDEQARMAFDYFLTAEDEVSRPELDAWVHTELTRRLPDAADDGLAAWNAYVEYR
                     SAAAATLADDTTLDAAEQRLAALISEHLGDYPIAAREQADNTRAFALKRAAALAGEAR
                     GLALAGLAAGSEPADAFLVGRRAAELARLTSADELHAVRTQHFGAEAADRLAALDARR
                     AAWNARLAAFQTARAALLVDFAGTQAQRATAEAELAAQHFSSTELPRVHALMRITAD"
     gene            complement(3444..4316)
                     /locus_tag="VGB85_01960"
     CDS             complement(3444..4316)
                     /locus_tag="VGB85_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132805.1"
                     /translation="MDDMNYRLGCIAWVWLCGCGPGKGSEDPASEGSSGGSTGAPTTA
                     GESGSPTTGASTEVAESGTTTGTSTGDESGTGTSTGTGESVLLGCAGLAPDVMVPASS
                     TLDGAIVSAVGAGARFEVPAQWLQWQAEHGHNLHLSRPELEAVRVGVGDWDTEYAHVL
                     AGLFNYDCCAAHVGGDSWAGGVSYDDLQMRVYVLEDLPVDIVARALSTSWELVTPKVT
                     VEEGDPWTRVLIHYTVWYGDYGGDAFVDLRLRRYEAATIVYAGMYSGDFDAEAEFDAI
                     VAGECWTPGTGECL"
     gene            complement(4358..6919)
                     /locus_tag="VGB85_01965"
     CDS             complement(4358..6919)
                     /locus_tag="VGB85_01965"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0132806.1"
                     /translation="MSETSAEPAAIGPFVVLRRLGEGAMGVVYAGYDVLLDRKVAIKL
                     VRPQLLGNPAIRERMVREAQAMARLSSAYVVQVFQVGEHEDSLYLAMEYIEGKTLTVW
                     LETRTRPWQVVLRTLCDAGRGLAAAHEAGLVHRDFKPDNVLVDASGHPRVLDFGLVRT
                     EVEGQYTADEAVSTGPDDAPPGSTMMPMMPMTPAEVAGRSRGQWATQLTQHGGAIGTP
                     AYMSPEQHFARTVGPKSDQFSFAITLYEALYGVRPFPGETWEKISYQVQLGTIPPPPP
                     RSRVPRRVFKVIARALATDPEQRWPSLDAMIATLERDPSRVRLRAALGVGLLGATVAT
                     TYVVARAVVPETQHCEASARELTGVWDPERRAALTKAFTATGVPFASDSLTRAAGRID
                     AYADAWVGEHRAACEAHASGVQTSRMMDLRVACLGRRKVHLQALVDLFGAADRAVVEN
                     AVQAVAALPSLHACADVDALLAGSAPPNDTRTADHVEQLRGQLARAAVLEHTGQFGRG
                     IELAAQVRGEAEALGYAPLTAEAALAVGRLYMAAARWAEADAALTEAPRLGIAHDLHA
                     VTAEAVTRRIFVVGAGLGHPAEALAVEPLAEALVQRARDDGRLSALLHNNLGAVYDLR
                     GDTHTARVYYQRTIAELQQRPGPSDPLIAITHNNIGNMDMDRGDFDSARAHFSAAKEL
                     FTTILGEGHPFVAHALAGLGDADARKGAHAEALGSYAQALGRMEAAYGSQHLYLLQPL
                     TGFGQVYARLGQADRAAPYFTRAVKIADDLGAVDVLLGRSLEGLADLAAAAGSHAEAI
                     NLYSRAARVYAETTGADANDSARVTGRANELAAAASVTPAVPGPQEGHVTGRGGR"
     gene            complement(6929..7885)
                     /locus_tag="VGB85_01970"
     CDS             complement(6929..7885)
                     /locus_tag="VGB85_01970"
                     /inference="COORDINATES: protein motif:HMM:NF039450.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADYC domain-containing protein"
                     /protein_id="HEY0132807.1"
                     /translation="MGSLVACDEDDASWAADAGVQDDQDDVTVRELEVNGFRLNGFRL
                     NGFRLNGFRLNGFRLNGDDDSDWIELEQIIVPNQPEIVGSWVVGSNLHLKTKYGTILS
                     GAQLVGAELDFGVSEALHGKKSKKVRITSVKPLKWGSDVLLYGLEIKDELSSWEPLCL
                     DQYDQGTQAILLTDAWDLETGDRITPKPSGVVTFACRDAALAKCVEWGYAPWRTVNNV
                     PLVNHHQTCTRAARADYCGDGTAHTYNGTKIHVLDKLGIQIPDLGLGLGYAVEAEWGP
                     NGAVCLNPNNTRLPDAEYACSPSKCSNVFNSGGLIQTGIPLL"
     gene            8297..>9274
                     /locus_tag="VGB85_01975"
     CDS             8297..>9274
                     /locus_tag="VGB85_01975"
                     /inference="COORDINATES: protein motif:HMM:NF025182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HEY0132808.1"
                     /translation="MKQAGHRLSFALLVSAGCFAGASPGPGETGDTTAASPTAGPGDT
                     TATSATAPTTTTSDDPPLPSGSTGQGTTTPDTDAITSTADTGTTAVEPPDDPIAMTRK
                     IADLTGPGTSAEQWGVGGTDLGIPVRQPDGQIAYIFGDTFEDHGVGGPGWRSPVLLRS
                     APGNLAAGVQFTGAAGGQYAKQILDYAHNDVHSTWLPTDAITIGDRMYLHYMVNKGLG
                     NVLWSEIAYSDDNGEHWVSSGTSWAGDENLSLRQLWTWERGGDGYVYTLSTAFTRDRG
                     IILHRVPEDQLLVKAAYEPWGFSGEWAWGNPATEVLTGKFGELCLRRVED"
BASE COUNT         1391 a         3538 c         2955 g         1390 t
ORIGIN      
        1 tccatcgcgc tgctcgctgc gggccgtgcc cgcgccgcca tggagggccg cgacttcgtc
       61 atccccgacg acatcaagag cctcgccccc tcggtcctgc gccaccgcct gatcttgcac
      121 cccgacgccg agttcgaggg gacctccgcc gacgactgca tccagaccgt gctcaccgag
      181 gcgcgcgtcc ccaggaccgc cgcctgacct gtcccgagag ccatgatccc ctcgcgcctg
      241 ttcgtgctgt tgatgctcgt cccgctggcc ctcgccatcg cggcgatcgc cgatcgcacc
      301 ttgctgtggc cgatgctcgg cgccgacggc ttcatcgcca tggtcgcggc gatggacgcc
      361 ctgctggccc ggcgcccgct cgtcgaggtc cgccgcacca gccgcgaggt gttctcgatc
      421 ggtcgcagca acgtcgtcac cctcgagctg cgctcgaagg cccgcagaag attgacggtg
      481 cgcgtcaccg acgacctctt cgagcacgcc agcgcccccg agctaccgct cgagctcgac
      541 ctgcgcggtg gttcccgcgc agaggccagc taccgcgtcg tcccgcaccg ccgcggcacc
      601 tacgcgctcg gcgaccacac gatccgctac cgctccccgc tcggcctgtg gatccgccag
      661 atcacccagc gcgccagcca gccggtgcgc atcttcccgg acgtccaggc cgtccgcgat
      721 tatgaattgc tcgcccgcca gagccgcgac gcggccgccc gcgcgagccg ccggcgcggc
      781 ggcgccagcg aattcgaggc cctgcgcgag taccagcgcg acgacgaata tcgcaagatc
      841 gactggaagg cgaccgcccg ccgccacaag ctcatcgccc gcacctacca gctcgagcgc
      901 gaccagagcg tcgtcttcat gctcgacgcc ggccgcctga tgaccgccga gagcgacgcg
      961 ctgtccctct tcgaccacgc cctcaacgcc acgctgatgc tgacatacgt ggcgagtcgc
     1021 ggcggcgacc aggtcggcct gatgacattc gccgaccggg tgctcacata tttgccgccc
     1081 ggccccggca agagcgcctc gcagcggctg atccgctcgc tgttcgccgt ccaccccgag
     1141 ctcgtcgaga ccgactacga gggcgccttc acctcgctcg gcccgcgcct gcgaaagcgc
     1201 agcctcgtcg tcctcttcac ccagatcctc gaccacaacg ccgcccagca gctcctgcga
     1261 ttgacccggg gtttattgcc ccgccacctc ccgctcgtcg tgctcctgcg cgaccccgcg
     1321 gtcgaggccc tcgtccagat cgagcccgat cagttcgccc tcggcgacca ccgccccttc
     1381 atcaccgccg ccgccgccga ggtcctcagc tggcgagaaa agctcatccg cgacctcagg
     1441 cgcgccggcg ccctcgtcct cgacgtcctc cctcgggaac tgtccccgag cctgatcaac
     1501 cgctacctcg aggtcaaagc ccggcaaatg ctgtgagccc gaggtctcgg ccccccatcc
     1561 gcatggggtg gatggcatgg ggtggatggc atggggcgga tgcagttaca tatttgggta
     1621 ctttactccg cgcgcgatcc cgtgtaggcg tgatgtcgtg cagttcccca agcgttggct
     1681 cccggcgagc ctggcaatcg ccgttctcgg caccttgtgc atcccgggca cggcctcggc
     1741 cggcgactat accaagacca agcacccgat cgtgctggcc cacggcatgt ccggcttcga
     1801 cgagctgttc ggcatctacg agtactggca gtcgatcccg caggctttga ttaaagacgg
     1861 cgccaaggtg ttcgtcaccg aggtcccgca gttcaactcg accgaggcgc gcggcgaggc
     1921 gctgctcgcc caggtcgagg agatcctcgc gatcaccggc gctgccaagg tcaacctgat
     1981 cggccacagc cacggcggcc tcgatgtccg ctacgtcgcc tcggtcgcgc ccggtctcgt
     2041 cgcgtccgtg acgaccgtcg gcagcccgca tcacggcgcc gagctggccg acttcctgcg
     2101 caacaacgtc aaggggggcg gcttcaccga ggtcgtgctc ggcctgttcg ccaacagcct
     2161 cggcaaggtg ctcgcgctgc tgtccggcgc gaacaaccaa caggacgcga tcgccgggct
     2221 cggggcgctg accaccgagg gcctgtcgac cttcaacgcc gcccaccccg cgggcctgcc
     2281 cgccaagtgg tgtggtgagg gccccgcggt cgtcggcggc atccacttct attcgtggac
     2341 cggtgaccgc gcgctcacca acatcctcga cccctccgac gccctgctcg gcctgacatc
     2401 gatcgtctac gacgaggaca acgacggcct cgtcggcaag tgcagcgccc accttggcaa
     2461 ggtcatccgc gacgactacc gcctcaacca cctcgacgag gtcaatcaag ccttcggcct
     2521 ggtccacctg ttcgggcccg accccaagag cctgtttcgc gtccacgcga accgcctgaa
     2581 gaacgagggg atgtgaccat gcgactccac cattttgtcc tcgtctccct caccctgctc
     2641 gccgcctgtt acagcggcgc ggaggctcag cccacgcaac cccgctcgct ggccggcacc
     2701 gagatcgacg gcgagctgcg gaccgagcgt ggtatgttcg tcttcgacga gcaggcgcgc
     2761 atggccttcg actacttcct caccgccgag gacgaggtca gccgccccga gctcgacgcg
     2821 tgggtccaca ccgagctgac ccgtcgcctg cccgacgcgg ccgacgacgg gctcgcagca
     2881 tggaatgcat atgtcgagta tcgctcggcc gccgccgcca cgctggccga tgatacgacc
     2941 ctcgacgccg ccgagcagcg actggccgcg ttgatctccg aacacctcgg cgactacccg
     3001 atcgcagcgc gcgagcaggc cgacaacacc cgcgccttcg ccctcaagcg agccgccgcg
     3061 ctcgccggtg aggcgagagg cctggcgctc gccgggctcg ccgcgggtag tgagcccgcc
     3121 gatgcgttcc tcgtcggccg ccgcgccgcc gagctcgcgc gcctcacgag cgccgacgag
     3181 ctgcacgccg tgcgcacgca gcacttcgga gccgaggccg ccgaccgcct cgccgcgctc
     3241 gatgcccgcc gcgccgcctg gaacgcccgc ctcgccgcgt ttcaaaccgc ccgcgccgcc
     3301 ctgctcgtgg acttcgccgg cacgcaggcg caacgcgcca ccgccgaggc cgagctcgcg
     3361 gcgcagcact tctcctccac cgagctccca cgcgtgcacg ccctcatgcg gatcaccgcg
     3421 gattgaacca gcctgcgagc aactcacaga cactcgccgg tgcccggcgt ccagcactca
     3481 ccggcgacga tcgcatcgaa ttcggcctcg gcgtcgaagt cgccgctgta catgcctgca
     3541 tatacgatcg tggcggcctc gtagcggcgc agtcgcagat cgacgaacgc atccccgcca
     3601 taatcgccat accacacggt atagtggatg agtacacgcg tccaggggtc gccttcctcg
     3661 acggtgacct tcggtgtgac cagctcccag ctcgtactca gggcccgcgc gacaatatct
     3721 accggtagat cttccagcac atatacacgc atctggagat cgtcgtagga tacgccgccg
     3781 gcccagctat cgccgccgac gtgagccgcg caacagtcat aattgaacag ccccgcgagc
     3841 acgtgggcgt attccgtgtc ccagtcaccg acgccgacgc gcacggcctc gagctcgggc
     3901 cgcgacagat gcaggttgtg tccgtgctcc gcctgccatt gcagccactg cgcggggacc
     3961 tcgaagcgcg ctccggcccc gacagcggac acgatcgcgc cgtcgagggt cgacgatgcg
     4021 gggaccatca catccggcgc gagcccggca caacccagga gcaccgactc gccggtcccc
     4081 gtcgacgtgc ccgtccccga ctcgtcaccc gtcgacgtgc ccgtcgtcgt cccggactcc
     4141 gcgacctccg tcgatgcacc cgtcgtcggc gaacccgact cgccagcggt cgtcggcgcg
     4201 cccgtcgatc ccccggacga cccctcactg gccggatctt ccgaaccctt cccgggtccg
     4261 cagccacaca gccagaccca cgcgatgcaa cccaggcgat agttcatgtc gtccacggtg
     4321 gcacgcgcag cggacctctg caagcgcgcc cggccgatca acggcccccg cgccccgtca
     4381 cgtgtccctc ctggggaccc ggcacggctg gcgtcacgga tgccgccgcc gcgagctcat
     4441 tcgcccgccc ggtcacgcgg gcgctgtcgt tcgcgtcggc gccggtggtc tccgcatata
     4501 cgcgtgcggc tcgactatat agattgatcg cctcggcgtg agatccggcg gcggcggcca
     4561 ggtccgcgag gccctcgagc gagcgcccga gcagtacatc gaccgcgccg aggtcgtcgg
     4621 cgatcttcac ggcgcgcgtg aagtagggcg ccgcgcggtc ggcctgcccg agccgcgcgt
     4681 agacctggcc gaagccggtc agtggttgca gcagatacaa atgttggctg ccatacgcag
     4741 cctccatccg cccgagcgcc tgggcgtagc tgccgagcgc ctcggcatgt gcgcctttgc
     4801 gcgcgtcggc gtcgccgagg ccggcgagcg cgtgcgcgac gaaggggtga ccctcgccga
     4861 ggatcgtcgt gaacagctcc ttcgccgcgc tgaagtgggc ccgcgcgctg tcgaagtcgc
     4921 cgcggtccat gtccatgttg ccgatgttgt tgtgtgtgat cgcgatcagc gggtcggagg
     4981 gcccgggccg ctgttgcagc tcggcgatcg tgcgttgata gtatacgcgc gcggtgtggg
     5041 tgtcgccgcg caggtcgtac acggcgccga ggttgttgtg cagcagcgcc gacagccggc
     5101 cgtcgtcgcg cgcgcgctgc accagcgcct ccgcaagcgg ctcgaccgcg agggcctccg
     5161 cgggatgccc gagccccgcg ccgacgacga agatcctccg ggtgaccgcc tcggccgtca
     5221 ccgcgtgcag gtcgtgggcg atgccgaggc gcggcgcctc cgtcagcgcc gcgtcggcct
     5281 cggcccagcg cgccgcggcc atgtacagcc gcccgacggc gagcgccgcc tcggcggtca
     5341 agggcgcgta gccgagggcc tcggcctcac cgcggacctg cgccgccagc tcgatcccgc
     5401 ggccgaattg tccggtgtgc tcgagcacgg ccgcgcgcgc cagctgcccg cgcaattgct
     5461 cgacgtggtc cgccgtccgc gtgtcgttcg gcggcgccga gccggcgagc agcgcgtcga
     5521 cgtcggcgca ggcgtgcagg ctcggcaagg ccgccaccgc ctgcaccgcg ttctcgacga
     5581 ccgcccggtc ggccgcgccg aacaggtcga ccagcgcctg aagatgcacc ttgcgccgcc
     5641 cgaggcacgc gacgcgcagg tccatcatgc ggctggtctg cacgccggac gcgtgcgcct
     5701 cgcaggcggc ccgatgctcg ccgacccatg catccgcata tgcatcgatg cgaccggccg
     5761 cccgcgtcaa gctgtcgctt gcgaacggca cgcccgtcgc cgtgaacgcc ttcgtcaacg
     5821 cggcgcgccg ctccgggtcc cacacgccgg tgagctcgcg cgcggaggcc tcacaatgct
     5881 gggtctcggg gaccaccgcg cgggcgacca cgtaggtggt cgccaccgtg gcgccgagca
     5941 gaccgacccc gagcgcggcg cgcaggcgca cgcgcgacgg gtcgcgctcg agcgtagcga
     6001 tcatcgcgtc gaggctcggc cagcgctgct cggggtccgt cgccagcgcg cgcgcgatca
     6061 ccttgaacac ccgtcgcggc acccgcgagc gcggcggcgg cggtgggatc gtgccgagct
     6121 gaacctgata gctgatcttc tcccaggtct ctccagggaa agggcgcacc ccgtacagcg
     6181 cctcgtacag cgtgatcgca aagctgaatt gatcggactt cgggccgacg gtgcgggcaa
     6241 agtgctgctc gggtgacatg tacgccggcg tcccgatcgc gccgccgtgc tgggtcagct
     6301 gcgtcgccca ctgcccgcgc gagcgacccg cgacctccgc gggcgtcatg ggcatcatgg
     6361 gcatcatggt cgagcccggc ggcgcgtcgt cgggccccgt cgagaccgcc tcgtccgccg
     6421 tgtactgccc ctccacctcc gtgcgcacga gcccgaagtc gaggacgcgc gggtgcccgc
     6481 tcgcgtcgac gagcacgttg tccggtttaa aatcgcggtg caccagcccc gcctcgtgcg
     6541 cggccgccag cccgcgaccg gcgtcgcaca gcgtgcgcag cacgacctgc cacggccgcg
     6601 tgcgcgtctc gagccacacc gtgagcgtct tgccctcgat gtactccatc gcgaggtaca
     6661 ggctgtcctc gtgctcgccg acctggaaca cctgcacgac gtacgcgctc gacaggcggg
     6721 ccatcgcctg ggcctcacgg accatgcgct cgcggatcgc agggttaccg agcagctgcg
     6781 ggcggaccag cttgatcgcg accttgcggt cgagcaggac gtcgtagccg gcgtagacga
     6841 cgcccatcgc cccctcgccg agccggcgca gcaccacgaa ggggccgatc gcagccggct
     6901 cggcgctcgt ctcgctcatt cgcaattctc acagcagcgg gatgccggtc tgaatgaggc
     6961 ctcccgagtt gaagacattg ctgcacttcg acggcgagca ggcgtactcc gcgtcgggca
     7021 agcgggtgtt gttcgggttg aggcagacgg ccccgttcgg cccccactcg gcctcgacgg
     7081 cgtagccgag gcccaggccg aggtcgggga tctggatccc cagcttgtcg agcacgtgga
     7141 tcttcgtgcc gttgtaggtg tgcgccgtgc cgtccccgca gtagtccgcg cgcgccgccc
     7201 gcgtgcacgt ctgatggtgg ttgacgagcg gtacattgtt gacggttcgc cacggagcgt
     7261 aaccccactc gacgcacttg gcgagcgcgg cgtcgcggca cgcgaaggtg acgaccccac
     7321 tcggcttcgg ggtgatgcgg tcgccggtct cgaggtccca cgcgtcggtg agcaagatcg
     7381 cctgcgtccc ctggtcgtac tggtcgaggc acagcggctc ccacgagctc agctcgtcct
     7441 tgatctcgag gccgtacagc agcacgtcgg acccccactt gagcggcttg acgctcgtga
     7501 tcctgacctt cttggacttc ttgccgtgca gcgcctccga gaccccgaag tcgagctccg
     7561 cgccgaccag ctgcgcgccc gagaggatcg tcccgtactt ggtcttgagg tgaaggttgc
     7621 tgccgaccac ccagctcccg acgatctccg gctggttggg cacgatgatc tgctcgagct
     7681 cgatccagtc gctgtcatcg tcgccgttga ggcgaaatcc gttgaggcga aatccgttga
     7741 ggcgaaaccc gttgaggcga aatccgttga gacgaaaccc gttcacctcg agctcgcgca
     7801 cagtgacatc gtcctggtcg tcctgtaccc cggcgtccgc cgcccacgac gcgtcgtcct
     7861 cgtcgcacgc gaccaggctc cccatactca ccaccgcgcc caataacccc gccaacgttc
     7921 gccgtgtcat ataccgagtt ccttcgtgtg cctctgctgt ggcagccaca ttcgaccgac
     7981 gcacgtgacc cacgcgacac gccaaaaccg cctccaaggc gtctcccgac aacctgcgta
     8041 acctggtgag accaacgcgg cgcttgtcag ctatcgcagc aggcgcgggc ccgtccgcgg
     8101 gcgatgccgc accgtcgccg tcgccgtcct gcgattcctc ctcacgggcc cggcggcccg
     8161 cgccgcccca ccaacctcgg ccgacgaggg acgacccgcg gagacccgct gcgcggtgcg
     8221 cgacgagctc ctcgttcacg gtactggcgc gatatctcgg tgtcgcagtg taccgaaaca
     8281 tgcgacgatc ccggccatga agcaagcagg gcaccgcctg tcgttcgctc tgctggtcag
     8341 cgccggctgc ttcgcgggcg cctctcccgg ccccggcgag accggcgaca ccacggccgc
     8401 cagcccgacg gccggccccg gcgacaccac cgcgaccagc gcgaccgccc cgacgaccac
     8461 gaccagcgac gacccgccgc tcccctcggg ctccaccggg caagggacca ccacgccgga
     8521 caccgacgcg atcacctcga cggccgatac cggcaccacc gcggtcgagc ccccggacga
     8581 cccgatcgcg atgacgcgaa agatcgccga cctgacgggc cccggcacca gcgccgagca
     8641 atggggcgtc ggcggcacgg acctcggcat tccggtgcgc cagccggacg ggcagatcgc
     8701 atatatcttc ggcgacacct tcgaggatca cggcgtcggc gggcccggct ggcgctcgcc
     8761 cgtgctgctg cgctccgcgc ccgggaacct cgccgccggc gtccagttca ccggcgcggc
     8821 tggcggccag tacgccaagc agatcctcga ctacgcgcat aacgacgtcc actcgacctg
     8881 gctgccgacc gacgcgatca cgatcggcga ccggatgtac ctgcattata tggtcaacaa
     8941 gggcctcggc aacgtgctct ggtcggagat cgcgtactcc gacgacaacg gcgagcactg
     9001 ggtgtcgagc ggcacgagct gggccggcga cgagaacctc tcgctgcgcc agctgtggac
     9061 ctgggagcgc ggcggcgacg gttatgtgta caccctgtcg accgccttca cccgcgaccg
     9121 cggcatcatc ctccaccgtg tgcccgagga ccaattgctc gtcaaggccg cctacgagcc
     9181 gtggggcttc tccggcgagt gggcgtgggg caacccggcc accgaggtcc tgaccggcaa
     9241 attcggcgag ctgtgcctgc gccgcgtcga ggat
//
LOCUS       DASWNJ010000106         4579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_1965, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000106 DASWNJ010000000
VERSION     DASWNJ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1965"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /locus_tag="VGB85_01980"
     CDS             <1..1479
                     /locus_tag="VGB85_01980"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0132809.1"
                     /translation="PGRDDGPIADHFAQAGLLAEAIDPAIRAARAARDLAGNVEAYHY
                     WSLALRAIGPADPRRWEALLAREAILQAWGRRRAQGADIRQILQLAEVTGDREREAVA
                     LGRLLRFYLECGRVARAEGLYPRLERTVADLPADESSAPRRAELGELGCDLMTRLGHL
                     EQAEALARAALEHCPPGPLGVLQRCRLHTGIGRVQLARGRLAEATASFEATLELARGA
                     GHRRAEAEALNSLGEVVGLSARYQEAVDHFRAALQIDRDLGDRAATGIKLANLGIAYT
                     AIGLYRRAERHLRKALELHAAIGHPGLLTEVVVHLGEVSAELGDPAAARPLLEEAASM
                     AAARGDIRTELRARSRLARCLLDAGQPDEAGAIAQAVLARGREQGLRSAQTRALHVLS
                     RLAEDAGDHAEAVAAEEEAVGLVRVGAAPLDGVLSIHHLGKLTGRTDLLEEAAARVRA
                     RLDDLRDPELRRGYLAQRRVQAILADAGPAAAGEPTPEKPGP"
     gene            1667..2209
                     /locus_tag="VGB85_01985"
     CDS             1667..2209
                     /locus_tag="VGB85_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132810.1"
                     /translation="MAVMTAAAACIPHGEIVATDGSRRMVARDTQSGLSVVLTTGAWD
                     GSPTDLDQDITVVHALVANMGATPVRLAPGDLDLIDERGFRHQLLDAGGSFLQTGEID
                     RGYHPGRSLDYGRIEWSGGDVNSSALPWGTLAPGTQMRGYLYFRRLENTANAATLTWH
                     FYSDKNAPVVDLAFDFFVAR"
     gene            2228..3697
                     /locus_tag="VGB85_01990"
     CDS             2228..3697
                     /locus_tag="VGB85_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132811.1"
                     /translation="MTTPVPGKARVPTCPSGQIARGPTAALTTVTTTVACAAATTVAC
                     AAAVTLPAVAHASNPWLTPLGAEAAASGGAVAAMGRDTGMAFYNPAGLGANRRAQFDM
                     STTMAVLRLRTIPGAFQIDLPGGRSQSAQLSSIEPLALATSTVYTRYMGHGVTVGAGH
                     FANNFDRYDYTGILEDRTGTSGLNYAARVQVDGWATRHYFGPSVGWQIMPRLRIGMSL
                     FITYEFQRDEGRIWMQAGPTPGLIDQNFIAGDIDLKRQTFGTEAVLGLQWEFVHRWHL
                     GLAFRTPRLAFLERARKTSLTTGAVAREDDTTAALFNFDDTPNAARRGRVSPLQIVLG
                     IAYAFPRARGWISAEADYTPAMFVPKNNVDLRHNVNARIGARIRVAERVMFGLGVFTD
                     RTNEKSINDFPQFNLDYYGGSMGIELRRPFKLGKTERAKDIVFATAVALRYAWGTGSS
                     GAVRFNLAESTNKLSYSIGEVHPVQFHVWSGHIGAGLYF"
     gene            complement(3735..>4579)
                     /locus_tag="VGB85_01995"
     CDS             complement(3735..>4579)
                     /locus_tag="VGB85_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132812.1"
                     /translation="YVAGGSAFRDQCMPISHATGNGVTQCGYVHKNYCPGGEGEQQSS
                     YQELLGVFGPRAEDTTKPTLVSFAPEDGATFTTADTFNVTASFTDDSNFIGVNWTWIE
                     GLPADLQADGYRRCTNDVCNDSYSAWKPVDDPWDFINLKQPPAGNYEFKVEAMDAYGN
                     HVAQNIRVTVVEAEGTTTASDTGSDSSDTSDSAGTEGSGSDSTAGGSATISGGLDDSG
                     DVPTEGGGDGGLTGGLDGGDSSSGGEDTAGGGSGGGCRVAPTPGAASLLALLLLGLRR
                     RRRA"
BASE COUNT          606 a         1774 c         1574 g          625 t
ORIGIN      
        1 cccggccgcg acgacggccc gatcgccgac cacttcgcgc aggccggctt gctcgcggag
       61 gcgatcgacc cggcgatccg ggccgcccgg gccgcccgcg acctcgccgg caacgtcgag
      121 gcgtatcact actggtcgct cgcgctgcgg gcgatcggcc ccgccgaccc gcgccgctgg
      181 gaggcgctgc tcgcccgcga ggcgatcttg caggcctggg gccgccggcg ggcccagggc
      241 gccgacatcc gccagatcct ccagctcgcc gaggtcaccg gcgaccgcga gcgcgaggcc
      301 gtcgcgctcg gccgactgct gcggttttat ctcgagtgcg ggagggtggc ccgcgccgag
      361 ggcctgtatc cccggctcga gcgcaccgtc gccgacctgc cggccgacga gagcagcgcg
      421 ccgcgccgcg ccgagctcgg cgagctcggc tgcgatctga tgacccgcct cgggcacctc
      481 gagcaggccg aggcgctcgc ccgcgcggcc ctcgagcact gcccgcccgg cccgctcggc
      541 gtgctgcagc gctgccgcct gcacaccggg atcggcaggg tccagctggc acgcgggcgc
      601 ctggccgagg cgaccgcgag cttcgaggcg acgctcgagc tggcccgcgg cgccggtcat
      661 cgccgggccg aggccgaggc gctcaacagc ctcggcgagg tcgtcggcct cagcgcgcgc
      721 taccaggagg cggtcgacca cttccgggcc gcgctgcaga tcgaccgcga cctcggcgac
      781 cgcgccgcga ccgggatcaa gctcgccaac ctcggcatcg cgtacacggc gatcggcctg
      841 tatcgccgcg ccgaacgcca cctgcgcaag gccctcgagc tgcacgccgc gatcggtcac
      901 ccgggattac tgaccgaggt cgtcgtgcac ctcggcgagg tctcggccga gctcggggac
      961 ccggccgccg cgcgaccctt gctcgaggag gccgcgagca tggccgcggc ccgcggggac
     1021 attcgcacgg agctgcgcgc ccgctcgcgc ctggcccgct gcctgctcga cgccggccag
     1081 cccgacgagg ccggcgcgat cgcccaggcc gtgctggccc gcggccgcga gcagggcctg
     1141 cgctcggcgc agacccgggc gctgcacgtg ctctcgcggc tggccgagga cgccggtgac
     1201 cacgcggagg cggtcgctgc ggaggaggag gcggtgggtc tggtgcgggt cggcgcggcc
     1261 cccctcgacg gtgtgctctc gatccaccac ctgggtaagt tgaccgggcg cacggacctg
     1321 ctcgaggagg ccgccgcccg cgtgcgcgcc cgcctggacg acctgcgcga cccggagctg
     1381 cgccgcgggt acctcgcaca gcgacgggtc caggcgatcc tcgccgacgc cggcccggcg
     1441 gcggctggcg agccgacgcc ggaaaaaccc ggcccgtgag cgccgccccg cccgcgcggg
     1501 gcagtttgtt tgcggcctcc ggggcacgcc tgggctgccc cccggggccc ccaccggcga
     1561 gcgcgggcct gctgcgcgca accggggtat gctggcggcc gtgcttcgcc ccgctcgcag
     1621 tcgccggact ccatggttcc tgggttgcgt cctcgctgcc tccgtgatgg ccgtgatgac
     1681 agccgccgcc gcgtgcatcc cccacggcga gatcgtcgcc accgacggca gccgtcgcat
     1741 ggtcgcccgc gacacgcagt cgggcctgtc ggtcgtgctg acgaccggcg cctgggacgg
     1801 cagccccacc gacctcgacc aggacatcac cgtcgtgcac gcgctggtcg ccaacatggg
     1861 cgcgaccccc gtgcgcctgg cccccggcga cctcgacctg atcgacgagc gaggctttcg
     1921 ccaccagctg ctcgacgccg gtggcagctt cctgcaaacc ggcgagatcg accgcggcta
     1981 ccaccccggc cgcagcctcg actacggccg catcgagtgg agcggcgggg acgtcaacag
     2041 ctcggcgctg ccgtggggca ccctcgcccc gggcacgcag atgcgcggct acctgtactt
     2101 tcgtcgcctc gagaacaccg ccaacgcggc gaccctgacc tggcacttct acagcgacaa
     2161 gaacgcgccg gtcgtcgacc tcgccttcga cttcttcgtc gcccgctagt cggagagccc
     2221 cgcaacgatg acgacaccgg tccctggcaa ggctcgcgtg cctacctgtc cctcaggcca
     2281 gatcgcgcgc ggcccgacgg ccgccctcac gactgtcacc accactgtcg cctgcgcggc
     2341 cgccaccacg gtcgcctgcg cggccgcggt cacgctgccg gcggtcgccc acgcgagcaa
     2401 tccgtggctg accccgctcg gcgccgaggc ggccgcctcg ggcggggcgg tggccgcgat
     2461 gggccgcgac accggcatgg ccttttacaa cccggcgggc ctcggcgcca accggcgggc
     2521 ccagttcgac atgtcgacga cgatggccgt gctgcgcctg cgcacgatcc ccggcgcctt
     2581 ccagatcgac ctgcccggcg ggcgcagcca gagcgcccag ctctcgagca tcgagccgct
     2641 cgcgctcgcg acctcgaccg tctacacccg ctacatgggc cacggcgtga ccgtcggcgc
     2701 cggccacttc gccaacaact tcgaccgcta cgactacacc ggcatcctcg aggatcgcac
     2761 cggcacgtcc ggcctcaact atgcggcgcg cgtgcaggtc gacggctggg cgacgcgaca
     2821 ctacttcggt ccgagcgtcg gctggcagat catgccgcgc ctgcggatcg gcatgtcgct
     2881 gttcatcacc tacgagttcc agcgcgacga gggccggatc tggatgcagg ccggaccgac
     2941 cccgggcctc atcgaccaga acttcatcgc cggcgacatc gacctcaagc gtcagacctt
     3001 cggcaccgag gccgtgctcg gcctgcaatg ggagttcgtc caccgctggc acctcggcct
     3061 cgcgttccgg accccgcgcc tggcgttcct cgagcgcgcg cgcaagacct cgctgacgac
     3121 cggcgccgtc gcccgcgagg acgacacgac cgcggccctg ttcaacttcg acgacacccc
     3181 gaacgcggct cgccgcggtc gcgtctcgcc gctgcagatc gtgctcggca tcgcctacgc
     3241 gttcccccgc gcccgcggct ggatcagcgc cgaggccgac tacacgccgg cgatgttcgt
     3301 gcccaagaac aacgtcgacc tgcgccacaa cgtcaacgcc cgcatcggcg cccggatccg
     3361 cgtcgccgag cgcgtgatgt tcggcctcgg cgtgttcacc gaccgcacca acgagaagtc
     3421 gatcaacgac tttccgcaat tcaacctcga ctactacggc ggctcgatgg gcatcgagct
     3481 gcgccgcccg ttcaagctcg gcaagaccga gcgcgccaag gacatcgtgt tcgccaccgc
     3541 cgtcgccctg cgctacgcct ggggcaccgg ctccagcggc gcggtgcgct tcaacctcgc
     3601 cgagtcgacc aacaagctgt cctacagcat cggcgaggtc catccggtcc agttccacgt
     3661 gtggagcggc cacatcggcg ccgggctcta cttctgagcc cggtcagcgg ccgtgagctt
     3721 cgggcacgcc gtctctaggc gcgccggcgg cgacgcaggc cgagcagcag caaggcgagc
     3781 aaggaggccg cgcccggcgt cggggcgacc cggcagccgc cgcccgatcc cccgcccgcg
     3841 gtgtcttcac cgcccgagct cgagtccccg ccatcgagcc cgcccgtgag cccgccgtcg
     3901 ccaccgccct cggtgggcac gtcgccgctg tcgtccagcc caccgctgat cgtcgccgat
     3961 ccacccgcgg tcgagtcgct cccggagccc tcggtgcccg cggagtccga ggtgtccgag
     4021 gagtccgagc cggtatcgga ggcggtggtg gtgccctcgg cctcgacgac cgtgacgcgg
     4081 atgttctggg cgacgtggtt gccgtaggcg tccatcgcct cgaccttgaa ctcgtagttg
     4141 ccggcgggcg gctgcttgag gttgatgaag tcccacgggt catcgaccgg cttccacgcg
     4201 ctgtagctgt cgttgcagac atcgttggtg cagcggcggt agccgtcggc ctggaggtcc
     4261 gcgggcaggc cctcgatcca ggtccagttg acgccgatga agttggagtc gtcggtgaag
     4321 ctcgccgtga cgttgaacgt gtccgcggtc gtgaaggtcg cgccgtcctc gggggcgaag
     4381 ctgacgagcg tcggcttggt cgtgtcctcg gcgcgcgggc cgaacacgcc gagcagctcc
     4441 tggtacgagc tctgctgctc gccctcgccg cccgggcagt agttcttgtg cacatagccg
     4501 cactgcgtca cgccgtttcc ggtcgcgtgg gagatcggca tgcactggtc gcggaacgcg
     4561 gagccgccgg cgacgtagg
//
LOCUS       DASWNJ010000107         1544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_249314, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000107 DASWNJ010000000
VERSION     DASWNJ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249314"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            586..843
                     /locus_tag="VGB85_02000"
     CDS             586..843
                     /locus_tag="VGB85_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132813.1"
                     /translation="MQPSPALSEHARPMQPSAALSEHARPALLEHAAPAMLGHAVSAR
                     LEHAGQARPMHPSTASKHTSSVQPSKRSEPGIPARGEGMRP"
     gene            840..>1544
                     /locus_tag="VGB85_02005"
     CDS             840..>1544
                     /locus_tag="VGB85_02005"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY0132814.1"
                     /translation="MMQILAFILLIGPLIFVHELGHLLAAKLVGVKVVRFSLGFGPAL
                     ARLRIGETEYRLAPIPLGGYVTLLGAAPDEPVSPQEQDRALRNKPLWARYLVLGAGPA
                     FNLILPLLLYFAFYLMHTVVAPPIIGTVLDDSAAAASDLQPGDRIVAIEGTDIHAWQE
                     MQDRVRVAPDKELRIQIEREGVRFDRFVTPRKNVVKNAVGEQESRGLLGVYPWVYAPQ
                     VGIIDTASPAYAEGLKT"
BASE COUNT          222 a          536 c          558 g          228 t
ORIGIN      
        1 tcgcgtcgac ggaagttgac ggggcgtctg cgcgtgaccc ctgggtcctc ccgctgcttc
       61 ccccgctcgt cggccccacc cggaccaaca tcctcggcgc cccgcttttc gccgctcgtg
      121 tgcgatcgcg aaggcggacg cccctcggcg ttgacgcgct ggtcgttgct cctcgacgac
      181 cctgtggccc gcgatgcttt tgtggtggcg agcgatgctt tgctcgccca cgacgcgcgg
      241 ctggactgcg accgtgtgac gcgttgcgag agccgggcgg tgcgtgccat cggagcggcg
      301 acggttcctg cgttcacgat gagcggggcg catacggtgc tcgcccgggg accgggggcc
      361 gacgagcgtg ggaagcagcg ggaggacccg gggctcgcgc gcaggcgccc cctcgtccgg
      421 tcggtcacgc gagacaggat cggtgaaagc gacatggctt tcgggacaga tggggcggcg
      481 agcacgtgct ccgggaggac ccgcgtcgcg gggacccgcc gcccgagggc ccccgctccc
      541 cggcccatga agcctcgccc cgcgttgtcc gagcacgcca ggcccatgca accgagcccc
      601 gcgttgtccg agcacgccag gccgatgcaa ccgagcgccg cgttgtccga gcacgccagg
      661 ccggcgctgc tcgagcacgc cgcacccgcg atgctcgggc acgccgtgtc cgcgcggctc
      721 gagcacgccg ggcaagccag gccgatgcac ccgagcacgg cgagcaagca caccagctcc
      781 gtgcaaccga gcaagcgaag cgagccgggg atcccggcca gaggggaagg aatgcgacca
      841 tgatgcagat cctcgcgttc atcctgctca tcgggccgtt gatcttcgtg cacgagctcg
      901 ggcatttgct cgcggcgaag ctggtcgggg tgaaggtggt gcgtttctcg ctgggcttcg
      961 gtccggcgct ggcgcggctg aggatcggcg agacggagta tcggctcgcg ccgatcccac
     1021 tcggcgggta cgtgacgctg ctcggcgctg cgccggacga gccggtcagc ccgcaagagc
     1081 aagacagggc gctgcgcaac aagccgctgt gggcccgtta tctggtgctc ggggccgggc
     1141 cggcgttcaa cctgatcctg ccgctgctgc tgtacttcgc gttttacctg atgcacacgg
     1201 tggtcgcgcc gccgatcatc ggcacggtgc tcgacgactc ggcggcggcc gcctcggacc
     1261 tgcaacccgg cgaccgcatc gtcgcgatcg agggcaccga catccacgcc tggcaggaga
     1321 tgcaggaccg cgtgcgcgtc gctcccgaca aggagctgcg gatccagatc gagcgcgagg
     1381 gggtccgctt cgaccgcttt gtcacgccgc gcaagaacgt cgtcaagaac gcggtcggcg
     1441 agcaggagtc gcgcgggctg ctcggggtct atccgtgggt gtacgcgccg caggtcggca
     1501 tcatcgacac ggcctcaccg gcgtacgcgg aggggctcaa gacc
//
LOCUS       DASWNJ010000108         3560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_284545, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000108 DASWNJ010000000
VERSION     DASWNJ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284545"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VGB85_02010"
     CDS             <1..391
                     /locus_tag="VGB85_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132815.1"
                     /translation="VVSWGHGDLALADDALVIRSAGVTSTIPLSELAHVEVTHVTGDS
                     GFADGNTLYYFVAIRPHAGPSRGIWLCEGHPYPLEKALFEPLRAAGLSSVVAVIDGPL
                     KGLSSSILWCAAVVWFVLAGLIVRALL"
     gene            complement(548..2233)
                     /locus_tag="VGB85_02015"
     CDS             complement(548..2233)
                     /locus_tag="VGB85_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0132816.1"
                     /translation="MPTATEAYRAARDQLLALRGDHARAVAEFTWPHLGDRFNWACDW
                     FDELARDAPSQKALIVVEQDGTTSELTFDGVYRRSHALAAWLREHGVRRGDAVVVMLG
                     NQVELWLTMLAVMRLGAVIMPTTTAVGATELIDRMTRGGARHVICNARDTALFDQVPG
                     DYTRISVGPRPGWHDLHGAPDEPVTLIGHPGTAPGDPLLLYFTSGTTSRPKLVQHTQV
                     SYPVGHLSTMYWIGLRPGDVHLNISSPGWAKHAWSCFFAPWIAGATIFVYNYARFDAH
                     ALLTHVRERRVTTFCAPPTVWRMLINADLSAGPGSLREIVGAGEPLNPEVIAQVQQKW
                     GLTLRDGFGQTEMTAAVGNTPGAPLKPGSMGRPLPGYPIVLVDPISGERVAAPGEGEI
                     CLDLATHPLPLMTGYHGDEQRNAEAMAGGFYHTGDVATLDPDGYITFIGRTDDVFKAS
                     DYKISPFELESVLIEHPAVAEVAIVPAPDPVRLAVPKAYVVLTPGHAPTRETAFAILK
                     YARERLAPYQRVRRLEFAELPKTLSGKIRRVELRAREPGDEWRDDMFPELRAP"
     gene            complement(2243..2941)
                     /locus_tag="VGB85_02020"
     CDS             complement(2243..2941)
                     /locus_tag="VGB85_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132817.1"
                     /translation="MNTWSPQQPQPVRPEPQNHVALGVLIALAVVGAALALLTALDRR
                     DTRDESTLGAAVTELHDARIELQASAAELRTATATMQEAARTLERRATTVNILDMIPP
                     PPAPPLPPTPLTAEGSQPAVDCAEEGRCTIARGELERLISNPSDLMRQARIIPSIKDG
                     QAQGLKLYAIRSTSFPAAIGFKNGDLLTAINGLEVRDTDATMKAYSALRNARALDFTI
                     ERKGQPVTLTLKIE"
     gene            complement(3054..>3560)
                     /locus_tag="VGB85_02025"
     CDS             complement(3054..>3560)
                     /locus_tag="VGB85_02025"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0132818.1"
                     /translation="VEGSLAESRRHSERALQILEKSLGREHDGLADVLCNLGRALVLQ
                     GELSQAGEHYARALALLERSHDANFAGLAKPLTGQGLLALAAGRPADALPPLERALAL
                     GIADPAKLAEARLALARALWDAKVDRPRARALAERARSELAIAGSGPMQARIGALLSE
                     WLAAHPVP"
BASE COUNT          483 a         1077 c         1442 g          558 t
ORIGIN      
        1 cgtcgtgtcc tggggtcacg gcgacctcgc gctcgccgac gatgcgctgg tgatccgcag
       61 cgctggcgtg acgagcacga tcccgctgtc ggagctcgcg catgtcgagg tcacccatgt
      121 caccggcgac agcggcttcg ccgacgggaa cacgctgtac tacttcgtgg cgatccgccc
      181 gcacgccgga ccctcccgcg ggatctggct ctgcgagggt catccatatc cgctggagaa
      241 ggcgctgttc gagccgctcc gcgcggcggg tttgtcgtcc gtggtcgcgg tcatcgacgg
      301 cccgctgaaa ggtttgagca gctcgatcct gtggtgcgcc gcggtcgtgt ggttcgtctt
      361 ggcggggttg atcgttcgcg cgctgctgtg agttgcggcc tgggaagcac gcccaccgac
      421 tccgcgcctc gggcggctcg atccggtggc gctactcgct gcggttgcat tcgtaccgcg
      481 acgagcccgg gccggtcgcc ttctcgctga agcggatgcc ccctgatatg gcaagtgtgc
      541 gcgctcgtca gggcgcgcgt agctcgggga acatgtcgtc gcgccactcg tcgccgggct
      601 cgcgcgcgcg cagctcgacg cggcggatct ttccggagag ggtcttgggg agctcggcga
      661 actcgaggcg gcgcacgcgt tgatagggag cgaggcgctc gcgggcatat tttaaaattg
      721 caaacgcggt ctcgcgggtc ggggcgtggc cgggcgtgag cacgacgtag gcctttggca
      781 cggcgaggcg cacggggtcc ggcgcgggca cgatggcgac ctcggcgacg gccgggtgtt
      841 ctatcaacac cgactcgagc tcgaaggggc tgatcttgta gtcggaggct ttgaagacgt
      901 catcggtgcg gccgatgaag gtgatgtaac cgtcggggtc gagggtggcg acgtcgccgg
      961 tatgataaaa accgccggcc atcgcctcgg cgttgcgctg ctcgtcgccg tgatagccgg
     1021 tcatcagcgg taaggggtgg gttgcgaggt cgaggcatat ctcgccctcg ccgggcgctg
     1081 cgacgcgttc gccggagatc gggtcgacga gcacgatcgg gtagccgggc agcgggcggc
     1141 ccatcgagcc cggcttgagc ggcgcgccgg gggtgttgcc gacggcggcg gtcatctcgg
     1201 tctggccgaa accatctcgc aacgtcaggc cccacttttg ctggacctgg gcgatgacct
     1261 cggggttgag cggctcgccg gcgccgacga tctcccgcag gctgccgggg ccggccgaga
     1321 ggtcggcgtt gatcagcatg cgccacacgg tcggcggggc gcagaaggtg gtgacgcgcc
     1381 gctcgcgaac gtgagtcagc agggcgtggg cgtcgaagcg ggcgtagttg tagacgaaga
     1441 tcgtcgcgcc ggcgatccac ggggcgaaga agcatgacca tgcatgcttg gcccagcccg
     1501 gcgaggagat attgaggtgc acgtcgcccg ggcgcaggcc gatccagtac atcgtcgaga
     1561 ggtgtccgac cggataggat acctgggtgt gctgcacgag cttcgggcgg ctggtggtgc
     1621 ccgaggtgaa gtagagcagc agcgggtcgc ccggggcggt gcccgggtgc ccgatcaggg
     1681 tgacgggctc gtcgggggcg ccgtggaggt cgtgccagcc gggccggggg ccgacgctga
     1741 tgcgggtgta gtcgccgggg acctgatcga agagggcggt gtcgcgggcg ttgcaaatga
     1801 cgtgtcgcgc gccgccgcgg gtcatgcggt cgatcagctc ggtggcgccg acggcggtgg
     1861 tcgtcggcat gatgacggcg ccgaggcgca tgacggcgag catcgtcagc cacagctcga
     1921 cctgattacc gagcatgacg acgacggcgt caccgcggcg caccccgtgc tcacgcagcc
     1981 acgcggcgag ggcgtgggag cggcggtaca cgccgtcaaa ggtcagctcg ctggtggtgc
     2041 cgtcctgctc gacgacgatc aacgcttttt gcgaaggagc gtcgcgggcg agctcgtcga
     2101 accagtcgca ggcccagttg aagcgatcgc cgaggtgcgg ccaggtgaac tcggcgacgg
     2161 cgcgggcatg gtcgccgcgc agggcgagca gctggtcgcg cgcggcccgg taggcctcgg
     2221 tcgccgtcgg catgccgaat gtctactcga tcttgagggt gagcgtcacg ggctggccct
     2281 tgcgctcgat ggtgaagtcg agggccctgg cgttgcgcag ggcgctgtag gccttcatgg
     2341 tcgcatcggt gtcgcggacc tcgaggccgt tgatcgcggt cagcaggtcg ccatttttga
     2401 agccgatcgc ggcggggaac gaggtgctgc ggatcgcgta gagcttgagg ccctgcgcct
     2461 ggccgtcctt gatcgagggg atgatccggg cctgacgcat caggtccgag gggttgctga
     2521 tcaggcgctc gagctcgccg cgggcgatcg tgcagcggcc ctcctcggcg cagtcgacgg
     2581 cgggctgcga gccctcggcc gtgagcgggg tcggcgggag cggcggtgcc ggcgggggtg
     2641 ggatcatgtc gaggatgttg acggtggtcg cgcgtcgctc gagcgtgcgg gcggcctcct
     2701 gcatcgtcgc ggtggcggtg cgcagctcgg cggcgctggc ctggagctcg atgcgggcgt
     2761 catgcagctc ggtgacggcg gcgccgaggg tcgactcgtc gcgggtgtcg cggcggtcga
     2821 gcgcggtgag caaggcgagg gccgcaccga cgacggcgag ggcgatcagc accccgagcg
     2881 cgacgtggtt ctggggctcg ggacggacgg gctgcggctg ctgcggggac caggtgttca
     2941 tgggaggatc cgtcgagtag caagaacggt accacggcgg tcgggtcggg aagaccggag
     3001 tcaatggcgc ggacttcgtg ccaccttgac acaggccggg cgagatgtgg acatcaaggc
     3061 accgggtgcg cggcgagcca ctcgctcagc agggcgccga tgcgggcctg catcgggccg
     3121 gagccggcaa tggcgagctc gctgcgggcc cgctcggcga gcgcgcgggc gcgggggcgg
     3181 tcgaccttgg cgtcccacag ggcgcgggcg agggcgaggc gggcctcggc gagcttggcg
     3241 gggtcggcga tgccgagcgc gagggcccgc tcgagcggcg ggagagcgtc ggccgggcga
     3301 ccggcggcga gggcgagcag gccctggccg gtcaatggtt tggcgaggcc ggcgaagttg
     3361 gcgtcgtggc tgcgctcgag cagggcgagg gcgcgggcgt agtgttcgcc ggcctgggac
     3421 agctcgcctt gcaggacgag cgcgcggccg aggttgcaca ggacgtcggc gaggccgtcg
     3481 tgctcgcggc cgagcgactt ctccaggatc tgcagggcgc gctcgctgtg ccggcgcgac
     3541 tcggcgagcg agccctcgac
//
LOCUS       DASWNJ010000109         1547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_178379, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000109 DASWNJ010000000
VERSION     DASWNJ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178379"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VGB85_02030"
     CDS             <1..183
                     /locus_tag="VGB85_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132819.1"
                     /translation="ASVVLVLVGVLVPGSPLSLPDVSPLAVIPLDSEAVPVDSSPSFV
                     LQAAVRLAAVTAQASR"
     gene            complement(552..>1547)
                     /locus_tag="VGB85_02035"
     CDS             complement(552..>1547)
                     /locus_tag="VGB85_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132820.1"
                     /translation="VLAGASAGRHLGRASTARQDIDARYHDPAMDPPHATDPPVLHPT
                     SSEVTIRSDGLAYARAMEIDPEQFARRILRPDRRRLAEVRARVGDMDDAREAWRVVAP
                     GSWLADDRRGFRGYCPHCKGSCLVGVPETRCPACRGYGEATLPSPEYTDAVVALCADP
                     AGVEAAESLARLAASRLSAWRAPAPSRIVWAVVCPTYWERSRWSSDFERALVTALAVV
                     KAGSEAATRAWSDALAEQLERSDRPCWGSAYLDAFGAACWRDAARLGEPIVRPPEGVP
                     GYFWVDAGAAVDGRTLDAVGDPFTPLAEIWALGYGLEIISDEAIVLVAPEIDQDE"
BASE COUNT          226 a          567 c          540 g          214 t
ORIGIN      
        1 gcgtcggtcg tgctcgtgct ggtcggcgtg ctcgtgcccg gctcgccgct ctcgctaccc
       61 gacgtatctc cgctggccgt catcccgctc gacagcgagg ccgtcccggt cgattcgtcg
      121 ccgtcgttcg tgttgcaggc cgccgtcagg ctcgcggccg tgaccgccca ggcgagcagg
      181 taagaaggat ctgccgaaaa aagcagcacg ccggggagca taaagaaacc tgcaccgatt
      241 taccacccga cgggcaccac cggcctggac gccgcgcagc ggatcgacct cggactcggc
      301 cgcggtgata agccctggca cgcgccataa cccagtcttc aatcgccttg cgaacagccg
      361 gatgggctca cgaccttgca ctcgcgcccg cgggagtgcc gtaggctcct ggcgctggtg
      421 gtcgcgtcga cgcccgcggg gctcgcggcg ttcgccaacg gcgaggcccg gttgaggcgc
      481 tgcgtcggaa tcagcgcccg ctgatggcga cgcgcggact ggccgcagtt tcgcgtgttc
      541 gtgcggagaa gtcactcgtc ctgatcgatc tcgggagcga ccagcacgat ggcctcgtcg
      601 gagattatct cgaggccgta gccgagcgcc cagatctctg ccagcggcgt gaatggatca
      661 cccacggcat cgagcgtgcg gccgtcgacc gcggcgccgg cgtcgaccca gaagtaccct
      721 ggcacaccct ccggcggccg cacgatgggc tcgccgagcc gggccgcgtc gcgccagcag
      781 gccgcgccga aggcgtccag gtaggcgctg ccccagcacg gtcggtcgct gcgctcgagc
      841 tgctcggcga gggcatccga ccacgctcgg gtcgcggctt cggagcccgc cttcaccacg
      901 gcgagcgccg tgacgagcgc ccgttcgaag tcggacgacc acctggagcg ttcccagtag
      961 gttgggcaca cgacggccca cacgatgcgc gacggcgccg gagcccgcca ggcgctcaga
     1021 cgcgaggcgg ccagacgcgc gagtgactcg gccgcctcga cacccgccgg gtcagcgcag
     1081 agcgcgacga cggcgtcggt gtactccggg gacggcaagg tcgcctcgcc gtatccccta
     1141 caggcagggc acctggtctc cggcacgccg accaggcagc tgcccttgca gtgcgggcaa
     1201 tatccgcgaa acccccgccg gtcgtccgcg agccagctcc ccggtgcaac cacgcgccag
     1261 gcctcgcgcg cgtcgtccat atcaccgacg cgcgcgcgca cctcggcgag ccgccgccgg
     1321 tcgggccgca ggatgcgtcg cgcgaactgc tccgggtcga tctccatggc tcgagcgtac
     1381 gccaggccgt cgctccggat cgtgacctcc gaggaggtcg gatgaagcac gggcgggtcc
     1441 gttgcgtggg gagggtccat cgccgggtcg tgataccgtg cgtcgatgtc ctggcgagcg
     1501 gtgctggccc gccccaggtg ccgccccgcg gacgcgccgg ccagaac
//
LOCUS       DASWNJ010000110         2815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_389495, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000110 DASWNJ010000000
VERSION     DASWNJ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389495"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..1163
                     /locus_tag="VGB85_02040"
     CDS             159..1163
                     /locus_tag="VGB85_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132821.1"
                     /translation="MAFALVAAVLMVFGPIPTASAESGTPAGWELSTATSEVIVGRVT
                     LRYDPSLHDEAMQLVDYIPGWWSEMEQALAGDLDDRLVINYVAHSGRIAEATGMPRWA
                     AGVAHSESGEIVIAQHAPDGSRTDLDSLLRHEMAHVALHRATDGQEIPRWFHEGVAES
                     FGNEIDLIRSQTLAGAVFGPGVPPLEQLEENFHGLDSVAATVSYAAARDFVNHLRGRD
                     SDGSDLRQVMSEIRRGTNFDAAFIKAYGRSLGELDSEWRSGLTGRFVWFPVVSSGGLP
                     LAALSPLMVVAAVRRRRQLREGWDRLGREDDALHAEVAEVLRRPGLLALPALNMAARA
                     "
     gene            1273..1854
                     /gene="rsmD"
                     /locus_tag="VGB85_02045"
     CDS             1273..1854
                     /gene="rsmD"
                     /locus_tag="VGB85_02045"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEY0132822.1"
                     /translation="MQRVGAGTLRGRVLRPLPPTTGDVRPTGSKVREAIFDRLHLGLP
                     GAVVLDLFAGTGALAIEAISRGAARATLIEQHPKVLRFLAEQIREFALADRVRVVKGD
                     ARQVLRGSPVAAGGPFSLVLIDPPYEDTAALLPVVAPALATGWLLPDAIVVCEHDRTH
                     ALPAWPDGFVLEASRRYGQTGVDFLRWEGGASR"
     gene            1873..>2815
                     /locus_tag="VGB85_02050"
     CDS             1873..>2815
                     /locus_tag="VGB85_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0132823.1"
                     /translation="MPTIELQTNARAGMLSVSPTLAISARAGELKAQGKAVLNFSAGE
                     PDFRPPPAVTASLTRFLATHPVHYTPVGGMSELRDAAALELADYHGRPVSRAEVLISC
                     GAKHSLANLFMVTLAPGDEVVIPAPYWVSYVDMVKLGDGAPVIVGTRREHGWRLQPAD
                     LEAALTPRTRFIVIGNPTNPTGTGYSADELRALAEVMVARAPQCWLLVDDIYRRLVYG
                     DFVHASAFAALKNITEQIIVIDGVSKSYSMTGYRIGFMLAPAGVVSAASKLQGHMTSG
                     PATPSQVAALAALTDPSCEPVVREMHAAFTRRREFMLA"
BASE COUNT          363 a         1060 c          961 g          431 t
ORIGIN      
        1 ggtcgcaagc tgcggacggg cctgatcgcg ggcggggcga tcctggccgt cgggctagcg
       61 atcggcctcg tgctggccaa cagcttgcgc cgggagacgg tcgtgatcac agtgggtcca
      121 tgacccttgc ggcacgtttc gggcgctggt tcctctcggt ggctttcgcg ctcgtggcgg
      181 ctgtcctgat ggtcttcgga ccgatcccca cggcctcggc cgagtcgggc accccggcgg
      241 gctgggagct gtcgacggcg acgagcgagg tgatcgtcgg ccgcgtcacc ctgcgttacg
      301 accccagcct gcacgacgag gcgatgcagc tcgtcgatta catcccgggc tggtggtcgg
      361 agatggagca ggcgctggcc ggtgacctcg acgatcgcct cgtaatcaac tacgtcgcgc
      421 actcgggccg gatcgccgag gcgaccggca tgccgcgctg ggccgcgggc gtcgctcact
      481 cggagagcgg cgagatcgtg atcgcccagc acgcgcccga cggctcgcgc accgacctcg
      541 actccctgct gcgccacgag atggcccacg tcgccctcca ccgcgccacc gatggccagg
      601 agatcccgcg ctggttccac gagggcgtcg ccgagagctt tggcaacgag atcgatctga
      661 tccgcagtca gaccctcgcg ggcgcggtgt tcggccccgg cgtcccgccc ctcgagcagc
      721 tcgaggagaa cttccacggc ctcgactcgg tcgccgccac cgtcagctac gcggccgccc
      781 gcgacttcgt caaccacctc cgcggccgcg actccgacgg ctccgacctg cgtcaggtga
      841 tgagcgagat ccggcggggc accaacttcg acgccgcgtt catcaaggcc tacggccgca
      901 gcctcgggga gctcgacagc gagtggcgca gcggcctgac cggtcgcttc gtgtggttcc
      961 ccgtcgtcag ctccggcggc ctgccgctcg ccgccctgtc gccgctgatg gtcgtcgccg
     1021 ccgtgcgtcg ccgtcgccag ctgcgcgagg gctgggaccg cctcggccgc gaggacgatg
     1081 ccctgcacgc cgaggtcgcc gaggtcctgc gccgccccgg cctgctcgcg ctgccggccc
     1141 tgaacatggc cgcccgcgcc tgatcgcagc ccgctcgcgt ggccccgcgg acctgtccgt
     1201 ggggccatgt tctttggttc acgccgccgt gatcgcttta caggtcagcc cggcctgggc
     1261 tactcccgcg ggatgcagcg agtcggcgca gggacgcttc gcgggcgggt gcttcgaccc
     1321 ttgccgccga cgaccggcga cgtgcgaccg accggctcca aggtgcgcga ggcgatcttc
     1381 gaccgcctgc acctcggcct gccgggggct gtggtcctcg atctcttcgc cggcaccggt
     1441 gccctcgcga tcgaggcgat cagccgcggc gccgcacgtg cgacgctcat cgagcagcac
     1501 cccaaagtgc tccgtttcct cgccgaacag atccgtgagt tcgcgctcgc ggaccgcgtc
     1561 cgggtcgtca agggcgatgc ccgccaggtc ctgcgtggat cgcccgtggc cgctggtggg
     1621 cccttctcgc tcgtgttgat cgatccgccg tacgaggaca cggccgccct gttgccggtt
     1681 gtggcgcccg cgctggcgac gggctggctg ctgccggacg cgatcgtggt gtgtgagcac
     1741 gatcgcaccc acgcgctccc cgcatggccc gacggcttcg tgctcgaggc ctcgcgccga
     1801 tacggccaga ccggggtgga ttttctccgc tgggagggcg gcgcgagccg ctaagctcgc
     1861 gggacgcacg acatgccgac gatcgaacta caaacgaacg cgcgggccgg catgctgagc
     1921 gtgtcgccca ccctggcgat ctcggcgcga gccggcgagc tcaaggcgca gggcaaggcc
     1981 gtcctcaact tcagcgccgg tgagcccgac ttccgtccgc cgccggccgt caccgcctcg
     2041 ctgacgcgct tcctcgccac ccacccggtc cactacacgc cggtcggcgg catgtcggag
     2101 ctgcgcgacg ccgcggcgct cgagctcgcc gactaccacg gccgcccggt gtcccgcgcc
     2161 gaggtcctga tctcctgcgg cgccaagcac agcctcgcca acctgttcat ggtgacgctc
     2221 gcgcccggcg acgaggtcgt gatcccggcg ccgtactggg tgtcctacgt cgacatggtc
     2281 aagctcggcg acggcgcgcc ggtgatcgtc ggcacccgcc gcgagcacgg ctggcgcctg
     2341 cagcccgccg acctcgaggc ggccctgacc ccgcgcaccc gcttcatcgt catcggcaac
     2401 ccgacgaacc cgaccggcac cggctactcc gccgacgagc tgcgggcgct cgccgaggtg
     2461 atggtcgccc gggccccgca gtgctggctg ctcgtcgacg acatctaccg ccgtctcgtc
     2521 tacggcgact tcgtgcacgc gagcgcgttc gcggcgctca agaacatcac cgagcagatc
     2581 atcgtcatcg acggcgtgtc gaagagctac tcgatgaccg gctaccggat cggcttcatg
     2641 ctcgcgccgg cgggggtcgt gtcggcggcg agcaagctgc aagggcacat gacctcgggg
     2701 cccgcgacgc cctcgcaggt cgcggccctc gccgcgctca ccgacccgag ctgcgagccg
     2761 gtcgtccgcg agatgcacgc ggcgttcacc cgccgccgcg aattcatgct cgcgg
//
LOCUS       DASWNJ010000111         2070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_108145, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000111 DASWNJ010000000
VERSION     DASWNJ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108145"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1310
                     /locus_tag="VGB85_02055"
     CDS             <1..1310
                     /locus_tag="VGB85_02055"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEY0132824.1"
                     /translation="LARPALLILSTFAVMAGTGALLLSFPAASRGQPLAFIDALFTAT
                     SASCITGLAVVDTASTFTDFGLAVMVVLLQLGGLGIMVLSTFATVLLGGRLALRSEQA
                     LEEVLDLASPGSAYELTRFIVLSTLAIEAVGAGLLAWGFHVRGMELGQAVWAGVFHAV
                     ASFCHAGFSLWPDSLISFHDDGLVLGVHMTLMLVGSLGFSVMAALWLWMRGAKRRFSL
                     QTRVVLWMTLVLIVLGTLGYALLEWNASLHGLSAGWKWVHALFQSISLRSGGFNSVDF
                     SRLEPATVMLMMMWMFIGAAPGSTGGGIKVTTLAVMLAAIPALIRNQPRATLLRRAIP
                     LEIVYRAATIVTVAGLIVGVSLIVLLATHPIRFDWMAFEVISAVGTVGMSLGATAQLT
                     PLGKWVMIALMFIGRVGPTSLALAVGASRSSQVRFPEGRIMVG"
     gene            complement(1593..>2070)
                     /locus_tag="VGB85_02060"
     CDS             complement(1593..>2070)
                     /locus_tag="VGB85_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132825.1"
                     /translation="RFPAGSLDLRIRLAITAGALLEPGERELRMRNPIDLHGRFVAGT
                     FELTGEFPLAPGQVRLALDFRPRAHPPVAGFDPPARLHGGTDGLLLPHDPTVPSPLHD
                     ASDPDGDLRALHWVVDGTPGAGHIPPGEHDVQLWAVDARGALARSPTRTVTVLAAR"
BASE COUNT          249 a          663 c          807 g          351 t
ORIGIN      
        1 tcctcgcccg tccggcgctg ctgatcctct cgaccttcgc ggtgatggcc ggcaccgggg
       61 cgctgctgct gagcttcccg gcggcgtcgc gcggccagcc gctcgccttc atcgacgcgc
      121 tgttcacggc gaccagcgcg agctgcatca ccggcctggc ggtggtcgac acggcgagca
      181 cgtttaccga cttcgggctg gcggtcatgg tggtgctgct gcagctcggc ggcctcggga
      241 tcatggtgct gtcgaccttc gcgacggtgc tgctcggcgg gcgcctggcg ctgcgcagcg
      301 agcaggcgct cgaggaggtg ctcgacctgg cgagcccggg ctcggcgtac gagctgacgc
      361 gcttcatcgt gctctcgacc ctggcgatcg aggcggtcgg cgccgggctg ctcgcgtggg
      421 gcttccatgt ccgcggcatg gagctgggcc aggcggtgtg ggcaggggtg ttccacgcgg
      481 tcgcgtcgtt ttgccacgcc ggcttctcgc tgtggcccga ctcgctgatc tcgttccacg
      541 acgacggcct ggtgctcggc gtgcacatga ccctgatgct ggtcggcagc ctcggcttct
      601 cggtgatggc ggcgctgtgg ctgtggatgc gcggcgcgaa gcggcgcttc tcgctgcaga
      661 cccgcgtggt gctgtggatg acgctggtgc tcatcgtgct cggcaccctc ggctacgcgc
      721 tgctcgagtg gaacgcgtcg ctgcacgggc tgtcggccgg ctggaagtgg gtgcacgcgc
      781 tgtttcagag catctcgctg cggtccggcg gcttcaactc ggtcgacttc tcgcgcctcg
      841 agccggcgac cgtgatgctg atgatgatgt ggatgttcat cggcgcggcc ccgggcagca
      901 ccggcggcgg catcaaggta acgacgctgg cggtcatgct ggcggcgatc cccgccctga
      961 tccggaatca gccccgcgcg acgctgctgc gccgggcgat cccgctcgag atcgtgtacc
     1021 gcgcggcgac gatcgtgacg gtcgccgggc tgatcgtcgg ggtgtccctc atcgtcctgc
     1081 tggcgaccca ccccatacgc ttcgactgga tggccttcga ggtgatcagc gccgtgggaa
     1141 cagtcggcat gtcgctcggc gcgacggccc agctgacgcc gctcggcaag tgggtgatga
     1201 tcgccctgat gttcatcggc cgcgtcggac cgacgtcgct cgcgcttgcg gtcggggcct
     1261 cgcgcagcag tcaggtgcgc tttcccgagg ggcgtatcat ggtcgggtga gcgcgctcgc
     1321 gagaaatgaa gtcccggata tggcgagaag tgagagcgcg gggcgaggac agctgggccg
     1381 gaggggggac ggacggcggg tccctgcggg tccaccaggg aagatgctcg ccgccccatc
     1441 tgtcgtgatt accacgttcg tgtcaccgag cattttgtgc gcgaccggag gttgacgggg
     1501 cgtctgcgcg tgacccctgg gtcctcccgc tgcttccccc gctcgtcggc cccacccgga
     1561 cgcgccacac cgttgtcgtg acagcggaga catcagcgag cggcgaggac ggtgacggtg
     1621 cgcgtggggg agcgcgcgag ggcgccgcga gcgtcgacgg cccagagctg gacgtcgtgc
     1681 tcgccgggcg ggatgtggcc ggcgccgggg gtgccatcga cgacccagtg cagcgctcgc
     1741 agatctccgt ccgggtcgct cgcgtcatgc agcgggctcg ggacggtggg gtcgtgcggg
     1801 agcagcaggc cgtcggtgcc gccgtgcagg cgggcgggtg gatcgaagcc ggcgacggga
     1861 gggtgggcgc gcgggcggaa gtcgagggcg aggcggacct gtccgggcgc gagcgggaat
     1921 tcgccggtga gctcgaatgt gccagcgaca aagcgcccgt gcaggtcgat cgggttgcgc
     1981 attcgtagct cgcgctcgcc gggttcgagc agcgcgccgg cggtgatggc gaggcggatc
     2041 cggaggtcga ggctgccggc cgggaagcgg
//
LOCUS       DASWNJ010000112         2408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_531498, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000112 DASWNJ010000000
VERSION     DASWNJ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531498"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..984)
                     /locus_tag="VGB85_02065"
     CDS             complement(37..984)
                     /locus_tag="VGB85_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132826.1"
                     /translation="MRRLPCAYLPLVAALAGGCFNPAGSGETSAGSTGSPEVTSSSTG
                     DAPTTSTTSTTGAEPTTTTPTGSSGPESSSSEPASTGVLPGTDTTDASDTSSTTDEET
                     TDTGDTTGGAGICGNGAREPGEGCDDHNTNDGDGCSAVCRREDLVVFVTSESFSVGQI
                     MNLGVADNLCIGRSQLGSAVPGRAKGSQYIAWLSDGFTSAVERLVDSSVAYRRVDGTT
                     VANGTADLLDSDLLQPINVDENGVVTSEDRAVCGDAYAWTGTHAWGMTTADQCDQWVT
                     ETDESFATAGNMLSTTQTWSEAMYCTCDKKLRLYCIEVG"
     gene            complement(1131..2321)
                     /locus_tag="VGB85_02070"
     CDS             complement(1131..2321)
                     /locus_tag="VGB85_02070"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0132827.1"
                     /translation="MGYPLAGRMANLGYIQVVRHCNHFCGFCSNPTTPYTHTFESMRV
                     LVDDFVRRGYYGVILTGGEPTLHPELPAIAGYAAAQGLHVRMITNGSRLADPEFARAL
                     AQAGLRLVHVSVYSVRPAVEARLRGSEDTLERAFAAIDNAHRFGVEVNVNCVINRLNA
                     DHLDHNIRYFMAKHPQVRHFVWNNLDPSMGRAEVNQRQFTPRLADFEASLLRAMRLLH
                     ASGRSFRVEKVPLCYMGEFAWASTETRKIVKGEERIVHFLDDKQTVRQTSWEHRYAPG
                     CVQCRLRPICGGLFDRGDGYDPAELAPQFVDPEPVVRAIVGDPRDPSFIAGAPVRAQA
                     ASSGRSPETGPGGQALAVGERSPQGERLYAAKRRTEARKAGALGVAMEPEMPDPATKR
                     PGEG"
BASE COUNT          327 a          898 c          798 g          385 t
ORIGIN      
        1 cggtcgatct gcaacctttt cgcgtgatcg cccgcctcac ccgacctcga tgcagtacag
       61 gcgcagcttc ttgtcgcagg tgcaatacat cgcctcgctc caggtctggg tggtcgacaa
      121 catgttcccc gcggtcgcga acgactcgtc tgtctccgtc acccactggt cgcactggtc
      181 ggcggtggtc atcccccatg catgggtgcc cgtccacgcg tacgcgtcgc cgcagaccgc
      241 cctgtcctcg ctcgtcacga cgccgttctc gtcgacgttg atcggctgca gcaggtcgct
      301 gtcgagcagg tcggcggtgc cgttggccac cgttgtaccg tcgacgcggc gataggccac
      361 ggacgagtcg acgaggcgtt ccaccgcgct cgtgaacccg tcgctgagcc aggcgatgta
      421 ctgcgagccc ttcgccctcc ccgggaccgc cgaaccgagc tgagagcgtc cgatgcacag
      481 attgtcggcg actcccaggt tcatgatctg ccccacggag aagctctccg aggtcacgaa
      541 cacgacgaga tcctcgcgcc tgcacacggc gctgcaaccg tcgccgtcgt tggtgttgtg
      601 atcgtcgcag ccctcgccag gttcgcgcgc gccgttgcca cagatgcctg cgccgcccgt
      661 cgtgtccccg gtgtccgtcg tctcctcgtc cgtcgtgctc gacgtgtccg acgcgtccgt
      721 cgtgtccgtg ccgggcagca cgccggtcga ggccggctcg ctgctcgagc tctccgggcc
      781 gctcgacccc gtgggggtcg tcgtcgtcgg ctccgctcca gtcgtcgacg tggtcgaggt
      841 cgtcggtgcg tcccccgtcg acgacgacgt gacctccggg ctgccggtcg atcccgcgga
      901 ggtctcaccg ctgccggcag ggttgaagca gccgcccgcg agggcagcga cgagcggcag
      961 gtacgcgcag ggcaggcgtc tcatcggggt ggcagatgct aacgcagagc ccggccgcgg
     1021 tcatccggac ccgcgggtcc gcgagcgagc gcggcagcgc tgcaaaaatg gaccatatac
     1081 cagcgcagcc gtcccccggc ggtcccaagg gctaccgcgc gtcctcctgg tcacccctcg
     1141 ccgggccgct tcgtcgccgg atcgggcatc tccggctcca tggcgacccc gagcgcgccg
     1201 gccttgcggg cctccgtgcg ccgcttcgcc gcgtacagcc gctcgccctg cgggctgcgc
     1261 tcgccgaccg cgagcgcctg tcctccggga cctgtctccg gggacctgcc cgaagaggca
     1321 gcctgcgccc gcaccggcgc gcccgcgata aagctcgggt cgcgcgggtc cccgacgatc
     1381 gcccgcacga ccggctcggg gtcgacgaac tgcggcgcca gctcggccgg gtcgtagccg
     1441 tcaccgcggt cgaacaggcc gccgcagatc ggccgcagcc ggcactgcac gcacccgggc
     1501 gcgtagcggt gctcccacga agtttgccgg accgtctgct tgtcgtcgag gaagtgcacg
     1561 atgcgctcct cgcccttcac gatcttgcgc gtctcggtgc tcgcccacgc gaactcgccc
     1621 atgtaacaca gcggcacctt ctcgacgcga aagctgcggc cgctggcgtg cagcagccgc
     1681 atcgcccgca gcagcgaggc ctcgaagtcg gccaggcgcg gcgtgaattg ccgctgattg
     1741 acctccgcgc ggcccatcga gggatcgagg ttgttccaca cgaagtgccg cacctgcggg
     1801 tgcttcgcca tgaagtaccg gatgttgtga tcgaggtggt ccgcgttgag ccggttgatc
     1861 acgcagttga cgttgacctc gacgccgaag cggtgggcgt tgtcgatcgc cgcgaacgcc
     1921 cgctccagcg tgtcctcgct gccgcgcagg cgcgcctcga ccgcgggccg caccgagtac
     1981 accgacacgt gcacaagccg cagccccgcc tgcgccagcg cgcgcgcgaa ctccgggtcc
     2041 gcgagccgcg agccgttggt gatcatgcgg acatgcagcc cctgcgccgc ggcgtagccc
     2101 gcgatcgccg gcagctccgg gtgcagcgtc ggctcgccgc cggtcaggat caccccgtag
     2161 tagccgcgcc gcacgaagtc gtcgacgagc acccgcatcg actcgaaggt gtgcgtgtac
     2221 ggcgtcgtcg ggttcgagca gaacccgcag aagtggttgc agtgccgcac gacctggatg
     2281 tagccgaggt tcgccatccg ccccgcgaga gggtagccca ccacggacct ggtgtatgct
     2341 cgccgcgatg gccccgcgtc acctccccgt gctgccggcc acttcggcca ccgcggtcaa
     2401 cgcggtcc
//
LOCUS       DASWNJ010000113         2376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_249395, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000113 DASWNJ010000000
VERSION     DASWNJ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249395"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VGB85_02075"
     CDS             complement(<1..211)
                     /locus_tag="VGB85_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132828.1"
                     /translation="MAVGTGVSVASWSAPVVAASPVPVDVCARAGACGTIVGPVRVAG
                     QRTPLADTAVIAVPASSKRLRASADP"
     gene            complement(229..1335)
                     /locus_tag="VGB85_02080"
     CDS             complement(229..1335)
                     /locus_tag="VGB85_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132829.1"
                     /translation="MAGAATTTVAAVATVARVVAVAALAGCLPEGAPPWKIDHAIVAA
                     IRVDIAEPGPYGPTSPRPDRLVAAAMPGDKIRFTPFLIGPDGPLDAGDVAPAWFYCQA
                     TRCFDSVAQPEVLRGCATEPLGPASTCRIGTGATATLQLGELSSLGLFGDPPSVFMVS
                     GTPGGASTARCLERLRNIASATESLRDCILIVEPLPIGPVWRLFFLAAFLGLPDAIPL
                     DSIPIEAQSAEPSLYPEVRPFEVTITASDGEARELVAVSEDTVAVAPGDTINIRAPVD
                     PFDAQLYYRTLIGGDGLPVMQAQFESFSSSWLLTDAVDEPTYGVQEVTWEVPAGASGE
                     MYGYYLLSDTRSVVWGWLRFELSGVSGVPGVSGS"
     gene            1446..>2376
                     /locus_tag="VGB85_02085"
     CDS             1446..>2376
                     /locus_tag="VGB85_02085"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HEY0132830.1"
                     /translation="MTVTLDETLHPAWLAELRRAWTDFNARHLGGRLQPPVLQIDRGE
                     LRLGRWEPEARLLSLAHHHIWTHAWDEVLGTLKHEMAHQYVDEVLGGDERPHGPQFAR
                     ACQLLGIDARASGTPRDREADEQSKTLRRVQKLLALATSANRHEAEAAMAAANTLLLR
                     YNLELPQGGPPLGYGARRLGTSAAALPLDWKLVGAILAEFFFVECIWTTTYNPRRDRH
                     ERVLEVMGSEANLEMAGYVHDFLHASTDRLWRLEHRALDLPGSRKREYTAGVLLGFRD
                     KLRQERKRNAEVGLVWLGDADLHRFVRVRHPHIR"
BASE COUNT          369 a          928 c          789 g          290 t
ORIGIN      
        1 ccgggtctgc gctcgcgcgc agccgcttcg agctcgccgg cacggcgatc accgcggtgt
       61 cggcgagcgg cgtgcgctgc cccgcgaccc gcaccggccc gacgatcgtc ccgcacgcgc
      121 ccgccctggc gcacacgtcg accggcaccg gcgacgcggc gaccaccggc gccgaccacg
      181 acgccacaga cacacctgtc ccgaccgcca tggcccgaag gacatgtctc atgaaccaga
      241 taccccaggt accccggaga ccccagagag ctcgaagcgc agccagcccc acacgaccga
      301 gcgcgtgtcg ctgagcagat aatagccgta catctcgccg ctggcgcccg cggggacctc
      361 ccacgtcacc tcctgcaccc cgtaggtcgg ctcgtcgacg gcgtcggtca gcagccagct
      421 cgagctgaag ctctcgaact gcgcctgcat caccggcagc ccatcgccgc cgatcagcgt
      481 gcgatagtac agctgggcgt cgaaggggtc gacgggcgca cgaatattga tcgtatcacc
      541 gggcgcgacg gcgacggtat cctccgagac ggcgacgagc tcccgcgcct cgccatcgct
      601 cgcggtgatc gtcacctcga acgggcgcac ctcgggatac aggctcggct cggcgctctg
      661 cgcctcgatc gggatcgagt cgagcgggat cgcgtcgggc agcccgagga acgcggcgag
      721 gaagaacagg cgccagaccg gcccgatcgg cagcggctcg acgatcaaaa tgcaatctcg
      781 caggctctcg gtcgcgctgg cgatgttgcg caggcgctcg aggcagcgcg ccgtgctcgc
      841 gccgcccggt gtaccggaga ccatgaagac cgacggcgga tctccgaaca ggccgagcga
      901 gctcagctcg ccgagctgca gcgtcgcggt cgcgccggtg ccgatccggc aggtgctcgc
      961 cggcccgagc ggctcggtcg cgcagccccg cagcacctcc ggctgcgcga cgctgtcgaa
     1021 gcagcgcgtc gcctggcaat aaaaccacgc cggcgcgacg tccccggcgt cgagcggccc
     1081 gtcgggtccg atcaagaacg gggtgaaacg gatcttgtcg ccgggcatcg ccgccgcgac
     1141 caggcgatcc ggccgcggcg aggtcggccc atagggccca ggctccgcga tgtcgacgcg
     1201 gatcgccgcg acgatcgcgt ggtcgatctt ccagggcggc gccccctcgg gcaaacagcc
     1261 agccagcgcc gcgaccgcca caaccctggc gaccgtcgcg accgccgcaa ccgtcgtggt
     1321 cgccgccccc gccacggtcc ccctccgccg tagcctgtgc gaagtgcctg gcacgcgcgt
     1381 gcagtatatc ggcgacgtgc cgcggttgtc tcggctggcg gccctgtgcc attctccgct
     1441 cccgcatgac cgtgaccctc gacgagacgc tgcacccggc ctggctggcc gagctgcggc
     1501 gggcctggac ggacttcaac gcgcgccacc tcggcggtcg cctccagccg ccggtcctgc
     1561 agatcgaccg cggcgagctg cgcctgggcc gctgggagcc cgaggcgcgc ctgctgagcc
     1621 tcgcgcacca ccacatctgg acccacgcgt gggacgaggt gctcggcacg ctcaaacacg
     1681 agatggccca ccagtacgtc gacgaggtcc tcggcggcga cgagcgcccg cacggcccgc
     1741 agttcgcccg cgcgtgtcag ctgctcggca tcgacgcccg cgcgagcggc acgccgcgcg
     1801 accgcgaggc ggacgagcag agcaagaccc tgcggcgggt acaaaagctg ctcgcgctgg
     1861 cgacgagcgc caaccgccac gaggccgagg ccgcgatggc ggccgccaac acgctgctgc
     1921 tgcgttacaa cctcgagctg ccgcagggcg gtccgccgct cggctacggc gcccgccgcc
     1981 tcggcaccag cgccgccgcc ctgccgctcg actggaagct cgtcggagcg atcctcgcgg
     2041 agttcttctt cgtcgagtgc atctggacca ccacctacaa cccgcgccgc gaccgccacg
     2101 agcgcgtgct cgaggtgatg ggcagcgagg ccaacctcga gatggccggc tacgtccacg
     2161 acttcctgca cgccagcacc gaccgcctgt ggcgcctcga gcaccgcgcc ctcgacctgc
     2221 ccggcagccg caagcgcgag tacaccgcgg gcgtgctgct cggctttcgc gacaagctcc
     2281 gccaggaacg caaacgcaac gccgaggtcg gcctcgtctg gctcggcgac gccgacctcc
     2341 accgcttcgt ccgcgtccgc cacccgcaca tccgcg
//
LOCUS       DASWNJ010000114         1609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_143487, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000114 DASWNJ010000000
VERSION     DASWNJ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143487"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="VGB85_02090"
     CDS             <1..1130
                     /locus_tag="VGB85_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132831.1"
                     /translation="QIGDSGGNGGIGAAGVVAAGLIAASRGQRDAARDLMNNAAGFEA
                     ESAPRAARVVANEWLVADAAARGDWPTVMRLGVRRDVASAQTRFLAFVAARIRREALI
                     DEPSATDLGLRWLWWWAPGRRRTRALLLQALAIPRVLHPRRPAPKPEPVVAPLDPAEY
                     AGDPLAHAQALHALWVTASEPERSLTPTHLAELCAAWDVAFAGPALARLATRSLALGT
                     VTPADAALAQIRRTVSEELAALARRAGVPLTVLGDAGIGADAAFLLRGELLGEVEQRS
                     HALRQRTQQKRGLPAVEEMREWNALRRAYEQAALLGGLEVRRLMFPDIHLNVCNYAVW
                     LWNERKEYAMSTPLFRWLLAEAEAVGDEEAIALQRKNVGSK"
     gene            complement(1144..>1609)
                     /locus_tag="VGB85_02095"
     CDS             complement(1144..>1609)
                     /locus_tag="VGB85_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEY0132832.1"
                     /translation="GRAGTDSAALLAGRAPRPAISAVVLDLLELADRHYGSADLGMRF
                     IPWPSRLAIVVAGRVYRGIGVVLRARGGDALAGRAVVSPLGKLGWSVAALGRFAWTLL
                     PTPEPTHDATLHRLIADMPGADSGNARGRGRFAGFPRLLRDRLPRIDRSATT"
BASE COUNT          195 a          604 c          624 g          186 t
ORIGIN      
        1 gacagatcgg agacagcggc ggcaacggcg gcattggggc cgcgggcgtc gtcgcggccg
       61 gcctgatcgc ggcgagccgg ggccagcgcg acgcggctcg cgacctgatg aacaacgcgg
      121 ccggcttcga ggcggagagc gccccgcggg ccgcgcgggt ggtcgccaac gagtggctgg
      181 tcgccgacgc ggccgcccgc ggcgactggc cgaccgtcat gcgcctcggc gtccgccgcg
      241 acgtcgcgtc cgcgcagacg cgcttcctcg cgttcgtggc ggcccggatc cgccgtgaag
      301 cattgatcga cgagccctcg gcgaccgacc tcgggctgcg ctggctgtgg tggtgggcgc
      361 ccggccgccg gcgcacccgg gcgctgctgc tgcaggcgct ggcgatcccg cgggtgttgc
      421 atccgcgccg gccggccccg aagcccgagc ccgtcgtcgc tccgctcgac cctgccgagt
      481 acgccggcga ccctctggcc cacgcccagg ccctgcacgc gctgtgggtg acggcgtccg
      541 agcccgagcg cagcctgacg ccgacgcacc tggccgagct gtgcgccgcg tgggacgtcg
      601 ccttcgccgg cccggcgctc gccaggctgg cgacgcgcag cctggcgctc ggcacggtga
      661 cgccggccga cgcggcgctg gcccagatcc gtcgcacggt cagcgaggag ctcgccgcgc
      721 tggctcgccg cgccggggtc ccgctgactg tcctcggcga cgcgggcatc ggcgccgacg
      781 cggcgttcct gctgcgcggc gagttgctcg gcgaggtcga gcagcgcagc cacgccctgc
      841 gccagcgcac ccagcaaaag cgcggcttgc cggccgtcga ggagatgcgc gagtggaacg
      901 ccctgcgccg cgcctacgag caggccgcgc tgctcggcgg gctcgaggtc cggcgcctga
      961 tgttcccgga catccacctc aacgtgtgca actacgcggt gtggctgtgg aacgagcgca
     1021 aggagtacgc gatgtcgacg ccgctgttcc gctggctgct cgccgaggcg gaggcggtcg
     1081 gcgacgagga ggcgatcgcg ttgcagcgca agaacgtcgg cagcaagtga cctgaccttc
     1141 gggtcaggtc gtcgcgctgc gatcgatccg cggcaggcgg tcgcggagca gccgcgggaa
     1201 gccggcgaag cgcccgcggc cgcgcgcgtt gccgctgtcg gccccgggca tgtcggcgat
     1261 caggcggtgc agcgtcgcgt cgtgggtcgg ctcgggcgtc ggcagcagcg tccacgcgaa
     1321 gcgacccagc gccgcgacgc tccagccgag cttgccgagc ggcgagacga ccgcgcgtcc
     1381 cgccagcgcg tccccgccgc gggcccgcag caccacgccg atgccccggt acacccgccc
     1441 ggcgacgacg atcgccaggc gcgagggcca ggggatgaag cgcatgccga ggtcggcgct
     1501 tccgtagtgg cggtcggcga gctcgagcag gtcgagcacg accgccgaga tcgccggacg
     1561 cggcgcccgg ccggccagca gcgcggcgct gtcggtcccg gcccgcccg
//
LOCUS       DASWNJ010000115         1979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_143572, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000115 DASWNJ010000000
VERSION     DASWNJ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143572"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VGB85_02100"
     CDS             <1..648
                     /locus_tag="VGB85_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132833.1"
                     /translation="ATTRSRYEATPRVLDADLQRMARGDRGCLGDAMAGFGALGIIVF
                     GVLGTLGMVGFSWMIIGTLMLLAGFIMSAAAQVRSAPPRRRALTEGPLVLARVVKADP
                     ALYEPSDAVLPARVVFAPGPERRFDGEYLRALGQRMQGLLRVDSTPPELAAVAAVMRD
                     ANRVDTVRIPAALAGEDEAYLAVVAVDARRLPARRIEDGEITLIVDPRSGFAEHV"
     gene            783..1919
                     /locus_tag="VGB85_02105"
     CDS             783..1919
                     /locus_tag="VGB85_02105"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0132834.1"
                     /translation="MKIRSTVITLLALAPLACGAPKADTAAPEPAPAPAPVPVAKQVK
                     PIPEGFFVLTPQLTVKGVDQAVEFYTKAFGATKVLAMPGPDGKTMHAEIKIGDSIVFV
                     DEEMEGMKSPQALGGSPASLMVYVPSADQTFAAATTAGAKVVMPLEDQFWGDRYGSVV
                     DPFGHRWSIGTHIEELTSEQTLQRAAIVFGADGGKKGKKPTKASKKAPAEPAWKKVAG
                     APATGPVPTGYHSVTIALTVKDAADAIDFYKLAFGALEIERMPSPDGKVMHATLEFGD
                     SKLMLGDEFLEMGAKSATTLGGSPVAIHYYSPDVDATFTKVTGAGAKQMMPLTDMFWG
                     DRYGAVTDPSGFMWGIATHKEDVSPEQMAERLKAETARQQKPTT"
BASE COUNT          308 a          714 c          678 g          279 t
ORIGIN      
        1 gccaccaccc gcagccgcta cgaggcgacg ccgcgggtcc tcgacgccga cctgcaacgc
       61 atggcgcgtg gcgatcgcgg ctgcctcggc gacgcgatgg cggggttcgg cgcgctcggg
      121 atcatcgtgt tcggcgtgct cggcacgctc ggcatggtcg gctttagctg gatgatcatc
      181 ggcacgctga tgctgctcgc cggcttcatc atgagcgcgg cggcgcaggt ccggtcggcg
      241 ccgccgcggc gacgcgcctt gaccgaaggg ccgctggtgc tcgcgcgcgt cgtcaaggcc
      301 gacccggcgc tctacgagcc gagcgacgcg gtcctgccgg cgcgcgtcgt gttcgcgccg
      361 ggcccggagc gccgcttcga cggcgagtat ctgcgtgcgc tcggccagcg catgcagggg
      421 ctgctgcgcg tcgacagcac gccgcccgag ctcgccgcgg tcgcggcggt gatgcgtgac
      481 gccaaccggg tcgacaccgt gcggatcccc gccgcgctcg cgggtgagga cgaggcctat
      541 ctcgcggtcg tcgcggtcga tgctcgccgc ttgccggcgc gacggatcga agacggcgag
      601 atcacattga tcgtcgatcc gcgaagcggc ttcgccgagc acgtgtgaag cctgtcggtt
      661 cgctccagca tcgggggcat cgcggcgtca aaacggcggt ccggcgcctg gacattgctg
      721 cgcgcttttg ccaccctccg cgccccactc gccggccctc gcgccgggga aggacaccgc
      781 ccatgaagat ccgcagcact gtcatcacac tacttgccct cgccccgctc gcctgtggcg
      841 ccccgaaggc cgacaccgcc gcgccggagc cggcgccggc gcccgcgccc gtcccggtcg
      901 ccaagcaggt caagccgatc ccggagggct tttttgtcct cacgccgcag ctgaccgtca
      961 agggcgtcga ccaggccgtc gagttctaca ccaaggcgtt cggcgccacc aaggtgctcg
     1021 cgatgccggg ccccgacggc aagacgatgc acgccgagat caagatcggc gactcgatcg
     1081 tgttcgtcga cgaggagatg gagggcatga agtcgccgca ggcgctcggc ggctccccgg
     1141 ccagcctgat ggtctacgtc cccagcgccg atcagacctt tgccgccgcg acgaccgcgg
     1201 gcgcgaaggt cgtgatgccg ctcgaggacc agttctgggg cgaccgctac ggatcggtcg
     1261 tcgacccctt cgggcaccgc tggtcgatcg gcacgcacat cgaggagctg acctccgagc
     1321 agaccctgca gcgcgccgcg atcgtgttcg gcgccgacgg cggcaagaag ggcaagaagc
     1381 cgaccaaggc gagcaagaag gcccccgccg agcccgcgtg gaagaaggtc gccggcgcgc
     1441 ccgcgaccgg gcccgtgccg accggctatc acagcgtcac gatcgcgctg accgtcaagg
     1501 acgccgccga cgcgatcgat ttttataagc tcgccttcgg cgccctcgag atcgagcgca
     1561 tgccgtcccc ggacggcaag gtcatgcacg cgacgctcga gttcggcgac agcaagctga
     1621 tgctcggcga cgagttcctc gagatgggcg ccaagtcggc gaccacgctc ggcggctcac
     1681 cggtggcgat ccactactac agccccgatg tcgacgcgac cttcacgaag gtcaccggcg
     1741 ccggcgcgaa gcagatgatg ccgctcaccg acatgttctg gggcgaccgc tacggcgccg
     1801 tcaccgaccc gtccggcttc atgtggggca tcgccacgca caaggaggac gtgtcccccg
     1861 agcagatggc cgagcgcctc aaggccgaga cggccaggca gcagaagccg accacctgac
     1921 cgttcggaca tgaacaaagc gccaggtgct cgcacctggc gctttttcgt gccgcgacg
//
LOCUS       DASWNJ010000116         3482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_531709, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000116 DASWNJ010000000
VERSION     DASWNJ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531709"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VGB85_02110"
     CDS             <1..409
                     /locus_tag="VGB85_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132835.1"
                     /translation="GCHPCDANVCESHLIVVVTEPGGGPLQAGNYVIDLTVDGVTDAA
                     TCDIGPGGATATCSALAMADLSAPRDDSPSNPLERLTLTWRPTVPEVVKIHIEHDGVV
                     VLDTTVEPAYDPVDETCAPGCNQPSEDLVLDRG"
     gene            476..1432
                     /locus_tag="VGB85_02115"
     CDS             476..1432
                     /locus_tag="VGB85_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132836.1"
                     /translation="MGYDGSLNHITHMHRDSGGGSKRSSIRTYQLGPGSSREEFPCMF
                     LCDGCGYLCWTDPPCPACGKKAWIDLGFWNHAEALRSREEEERRHPPERLKWQVRMAA
                     VTAGSAIGLGSAAGLALGGVVLAAPLVLTLGAGASLLTYAFGRRQIGWSIMTRRVDHP
                     TRWHVPLPLADPGAKPVSQLLGAAHPTGALLRAPFSGRPCIGYDVGVVFDTPGDAWPP
                     IWVLREMHSCAFEIQGKTIDAGGVSLALPIKPVAEPAITGEQKKKFLRERGLFLADGK
                     FDLFEAIIEPGSTYEMLWPSAPGGAAPILRAAPQARAGDPYR"
     gene            1523..>3482
                     /locus_tag="VGB85_02120"
     CDS             1523..>3482
                     /locus_tag="VGB85_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132837.1"
                     /translation="MHRLALLLAFAVVAVPGLSASAAEVRVERPRLFLSDGSGPGITR
                     DTYVARCTGDAAYMARCQGSLGGDDSRFGAWAKAAKYVVTGDPTACAAAFTQAQAKAA
                     DNPGEKDAHSFISDNGTTMLSLAVTRDWCDAALAPAEIEWLEGRIVEYADWYLQNDPK
                     DIFHDDMNNVFSAVALAGLALKHGAQDAKADMYLAAAEAKWKDIILPGLAHVGDWWHE
                     GMTYVQPAVGTFAIYLMAWSTATDEDMYAYVESSAGDLANGYLRMHAQAIRPDWRYVY
                     FGDTADPKQSIELHSRWLVDMLNVGPASAFGQGLSMAIRDHSQPYYDYAGAEGYLMTL
                     LYDAGKEASAAPLSDQPRTAWLSQGANDIAVLRSGWGEDDTYLWISCGDYLGAHQHIE
                     AGSFQLFRHTMLSGATGNYDAFDSDHWQNYYSQHSVHANTLGIHQPGELFPTLYTLND
                     PAANVNDGGQRVLRMNEVGSKFPSVSLASYNENKQSGTKYETGDLELIEESACHSYVA
                     CDVTAAYTSPGYETNTNTAKVNEVTRQFVFLPPDLVVIFDRVESTDPAYDKRFLLHAL
                     AEPELAGDRFTITNGEGRLLGRTVLPQGAVVTAVHDFAVDGKPHPPQQGTLESGGTRL
                     EVSPAQESARDYFLHVLLATDAGQSEAPP"
BASE COUNT          549 a         1290 c         1146 g          497 t
ORIGIN      
        1 cggttgccac ccctgcgacg cgaacgtgtg cgagtcccac ctcatcgtcg tcgtcaccga
       61 gcccggcggc ggaccgttgc aggccggcaa ctacgtcatc gacctcaccg tcgacggcgt
      121 gacggacgcc gcgacctgcg acatcgggcc cggcggcgcc actgcgactt gcagcgcgct
      181 cgccatggcc gacctgtcgg ccccgcgcga cgactcgccg agcaacccgc tcgagcgctt
      241 gacgctcacc tggcggccca ccgtcccgga ggtggtgaag atccacatcg agcacgacgg
      301 cgtcgtcgtc ctcgatacga cggtcgagcc cgcgtacgat ccagtcgacg agacctgcgc
      361 cccgggctgc aaccagccga gcgaggacct cgtgctcgat cgcggctgac gcggcgaaca
      421 cccgcgcgtg acgtctcgtg acccgaccgt gctttcggtc tacgctgatc ttcgcatggg
      481 ttacgacggc tcgctcaacc atatcacgca catgcaccgc gacagcggcg gtggttcgaa
      541 gcgcagctcg atccgcacgt atcagctcgg ccccgggtcg agccgcgagg agttcccgtg
      601 catgttcctg tgcgacggct gcgggtacct gtgctggacc gacccgccgt gcccggcctg
      661 cggtaaaaag gcgtggatcg acctcggctt ctggaaccac gccgaggcgc tgcgcagccg
      721 cgaggaggag gagcgccggc acccgcccga gcgcctcaag tggcaggtcc gcatggccgc
      781 ggtcacggcc ggcagcgcga tcgggctcgg cagcgccgcc ggcctcgcgc tcggcggcgt
      841 cgtgctcgcc gccccgctgg tgctgacgct cggcgccggc gcgagcctgc tgacctacgc
      901 gttcggccgc cggcagatcg gctggtcgat catgacgcga cgcgtcgacc acccgacgcg
      961 ctggcacgtg ccgctcccgc tggcagatcc aggggcgaag ccggtctcgc agctgctggg
     1021 agcggcgcat ccgaccggcg ccttgctgcg cgcgccgttc tccggcaggc cgtgcatcgg
     1081 ctacgacgtc ggcgtcgtgt tcgacacccc cggcgacgcg tggccgccga tctgggtcct
     1141 gcgcgagatg catagctgcg ccttcgagat ccagggcaag acgatcgacg ccgggggcgt
     1201 atcgctggcc ctgccgatca agccggtggc cgagcccgcg atcaccggag agcagaagaa
     1261 gaagttcctg cgcgagcgcg gcctgttcct cgccgacggt aagttcgacc tcttcgaggc
     1321 gatcatcgag cccgggagca cctacgagat gctgtggccc tcggcccccg ggggcgcggc
     1381 cccgatcctg cgcgccgccc cgcaggcccg cgcgggcgac ccctatcgtt gagccgcttt
     1441 tgcgcacttg cgcccgcgac ggcacccagg cctccgcagg ccgacgctcc cccgcgcccg
     1501 cggcgtggtc taccctgcga acatgcatcg cctcgcgctc ttgctcgcat tcgccgtcgt
     1561 cgccgtcccg ggcctgtctg ccagcgccgc cgaggtccgc gtcgagcggc cgcgcctgtt
     1621 cctgtccgat gggtcaggtc ctggcatcac cagggacacc tacgtcgcgc gctgcaccgg
     1681 cgacgccgcg tacatggccc gctgtcaggg ctcgctcggc ggcgacgaca gccgcttcgg
     1741 cgcgtgggcc aaggccgcca agtacgtggt gaccggcgat ccgacggcct gcgccgccgc
     1801 gttcacgcag gcgcaggcga aggccgccga caaccccggc gagaaggacg cgcacagctt
     1861 catctccgac aacggcacca cgatgctctc gctggcggtg acgcgcgact ggtgcgacgc
     1921 cgcgctggcg ccggcggaga tcgagtggct cgaggggcgc atcgtcgagt acgccgactg
     1981 gtacctgcaa aacgacccca aggacatctt tcatgacgac atgaacaacg tgttcagcgc
     2041 ggtcgccctc gccggcctcg cgctcaagca cggggcccag gacgcgaagg cggacatgta
     2101 cctcgcggcc gccgaggcca agtggaagga catcatcttg ccgggcctgg cccacgtcgg
     2161 cgactggtgg cacgagggca tgacctacgt gcagcccgcg gtcggcacgt tcgcgattta
     2221 cctgatggcg tggagcaccg cgaccgacga ggacatgtac gcgtacgtcg agagcagcgc
     2281 cggcgacctc gccaacgggt atcttcgcat gcacgcccag gcgatccgcc ccgactggcg
     2341 ctacgtgtac ttcggcgaca ccgccgaccc gaagcagtcg atcgagctgc acagccgctg
     2401 gctggtcgac atgctcaacg tcggccccgc ctccgcgttc ggccagggcc tgagcatggc
     2461 gatccgcgat cattcgcagc cctactacga ttacgccggc gccgagggct atctgatgac
     2521 cctgctgtac gacgccggca aggaggccag cgccgcgccg ctgtccgacc agcccaggac
     2581 cgcgtggctg agccagggcg ccaacgacat cgcggtgctg cgctccggct ggggcgagga
     2641 cgacacctac ctgtggatct cctgcggaga ctacctcggc gctcaccagc acatcgaggc
     2701 cggcagcttc cagctgtttc gccacaccat gctcagcggc gcgaccggca attacgacgc
     2761 cttcgacagc gaccactggc aaaattacta ttcgcagcac tcggtccacg ccaacaccct
     2821 cggcattcac cagcccggcg agctgttccc gaccctgtat accctcaacg acccggccgc
     2881 caacgtcaac gacggcggcc agcgggtgct gcggatgaac gaggtcggca gcaagttccc
     2941 gagcgtcagc ctggcttctt ataacgagaa caagcagagc ggcacgaagt acgagaccgg
     3001 cgacctcgag ctgatcgagg agtccgcgtg ccacagctac gtcgcctgcg acgtcaccgc
     3061 ggcgtacacc tccccgggct acgagaccaa caccaacacc gccaaggtca acgaggtcac
     3121 ccgccagttc gtgttcctgc cgccggacct cgtcgtgatc ttcgatcgcg tcgagtcgac
     3181 cgacccggcg tacgacaagc gcttcttgct gcacgcgctc gccgagccgg agctcgcggg
     3241 cgaccggttc acgatcacca acggcgaggg ccggctgctc ggacgcacgg tgctgccgca
     3301 gggcgcggtc gtcaccgccg tgcacgactt cgcggtcgac ggcaaacccc acccgccgca
     3361 gcagggcacg ctcgagtccg gcggcacgcg gctcgaggtc tcgcccgcgc aggagagcgc
     3421 gcgcgactac ttcttgcatg tgttgctggc gaccgacgcc ggccagtccg aggcgccgcc
     3481 gg
//
LOCUS       DASWNJ010000117         2332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_531724, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000117 DASWNJ010000000
VERSION     DASWNJ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531724"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            381..1481
                     /locus_tag="VGB85_02125"
     CDS             381..1481
                     /locus_tag="VGB85_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132838.1"
                     /translation="MRAPILAALALAACGDAAAPGFSTGPAITSVSASTSGSSTGADT
                     SSTGPADDSAGSSASTSTSTGAATLDVGTVMDFGPGNQPPGCKGKVDLLFLISRIGTM
                     VTEQEQLLASFPGFIETIEQKLEGFDVHIMTANPDGIWPGKYVCEGANGGCSKSWPNC
                     GPTAQDYDCETYADLVTECDEDLGAGLTFNTGGSAANKLCNLDGGRRYIVGDEPDLPG
                     AFECIAKVGTSGDGPFLGDAMIAALSDKQNGVGGCNEGFLRDEALLVVVFISDHEDDS
                     KSYAKQQYEAIVAAKGDPNAVVMLAVTPQPLGDKEPVPGCTYDVPPLNFSELLSRFSY
                     TVVGDTCASTYAPFFDAAVDKISEACGSFIPQ"
     gene            complement(1500..>2332)
                     /locus_tag="VGB85_02130"
     CDS             complement(1500..>2332)
                     /locus_tag="VGB85_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132839.1"
                     /translation="LLDADGHATGIELEGTPGQVVRALAADAEGGCFAVGLAVVMGDW
                     DIAYWRIDAAGVPMLGDTFDYVPPGDVMQLPHKFMDLASDVVIDGDVAWVVGMSKGDH
                     DGGDPDVLRGILVPMSIHTGPLGLGSVAPADNGWPQSAFFGAATYPGGVITTGYRCDE
                     TCSKYEILTSRYADGKRVWFENDATTTGLAYGSDVVLDSQGRVLVAGAVTQNGKLRGY
                     VFGRKLDVLGPPLFEHWYPGAGPSEALGIARDGYDRIFPGGYITVNGEPQARITRIHG
                     "
BASE COUNT          390 a          798 c          773 g          371 t
ORIGIN      
        1 acccctagcg cgcccggatg agcttcctaa attggacgtt gatctgggcg agtcgttcgc
       61 cgtcacgttt gacggctaga ccgagttggt gtgaccgctc gcgctcagct cggacccggg
      121 aagctagggc ctccatgagc gcccgactga caacgtcacg ccctgtgccc catacggcgg
      181 caagaacctc gtcgccgttg tccgccgcgc cgacaaccgt atgaccatga ctcgtcaaca
      241 aggacgcgag atggttgcgg atttttgcag gcgccatgga cttccgatat tttcatgaac
      301 tcgcggcccg gacacgtagg taaaaccccg aggtcgcccg gcgtgacatc gcccgcggcg
      361 aggtctaccc ttgcggtgtg atgcgcgcgc ccatccttgc cgccctggcc ctagccgcct
      421 gcggggacgc tgccgcgccc gggttctcga ccgggccggc gatcacgagc gtgtccgcgt
      481 cgacgagcgg cagctcgacc ggcgccgaca catcgtccac cgggcccgcc gatgactcgg
      541 ccggcagctc cgcgagcacg agcacgagca caggcgcggc gacgctggac gtcgggaccg
      601 tgatggactt cgggcccggc aaccagccgc cgggctgcaa gggcaaggtg gacctgctgt
      661 tcttgatctc gcgtatcggc acgatggtga ccgagcagga gcagctactc gccagcttcc
      721 cgggcttcat cgagacgatc gagcagaagc tcgagggctt cgacgtccac atcatgacgg
      781 ccaacccgga cgggatctgg ccgggtaaat acgtgtgtga aggtgccaac ggcggttgct
      841 cgaaatcctg gccgaactgc ggcccgactg cccaagacta cgactgcgag acgtacgccg
      901 accttgtgac ggagtgcgac gaggatctcg gcgccggcct gacgttcaat acaggcgggt
      961 ccgccgcgaa caagctgtgc aacctcgacg gcggacgccg ctacatcgtc ggcgacgagc
     1021 cggacctgcc gggcgccttt gagtgcatcg ccaaggtcgg cacgtccggc gacggcccgt
     1081 tcctgggcga cgcgatgatc gccgcgctct cggacaagca gaacggggtg ggtggctgca
     1141 acgagggttt cctgcgcgac gaggcgttgc tcgtcgttgt gttcatcagc gaccacgagg
     1201 acgactcgaa gtcgtatgcc aagcagcagt acgaggcgat cgtcgcggcc aaaggggacc
     1261 caaacgccgt cgtcatgctc gcggtgacgc cgcaaccgtt aggggacaag gaacccgtgc
     1321 caggctgcac gtacgacgta cccccgctca acttctccga gctgttgtcg cggttctcgt
     1381 acaccgtcgt tggtgacacg tgcgcatcga cctacgcccc gttcttcgac gcggccgtcg
     1441 acaagatcag cgaggcgtgc gggagcttca tcccgcagtg acgacgcccg gcgcgcgcct
     1501 tacccgtgga tccgcgtgat ccgggcctgc ggctcgccgt tgacggtgat gtagcccccg
     1561 gggaagatgc gatcgtagcc gtcccgagcg atgccgaggg cctctgaggg cccggcaccg
     1621 gggtaccagt gctcgaacag cggcgggccg agcacgtcga gtttgcggcc gaacacgtag
     1681 ccacgcagct tgccgttctg cgtcacggcg cctgcgacga gcacacgacc ctgactgtcg
     1741 agcacgacgt cgctgccata cgcgaggccg gtggtcgtcg cgtcgttctc gaaccacacc
     1801 cgcttaccgt cggcgtagcg cgacgtcagg atctcgtact tcgagcaggt ctcgtcgcag
     1861 cggtagcccg tggtgatcac gcccccgggg taagttgcgg cgccgaagaa cgcgctctgc
     1921 ggccatccat tgtccgctgg cgcgacgctc ccaagcccga gcgggccggt gtggatgctc
     1981 atcggtacga ggatgccgcg gagaacatcc gggtcgccgc cgtcgtggtc ccccttgctc
     2041 atgccgacga cccacgcgac gtcgccgtcg atcacgacat cgctggcgag gtccatgaac
     2101 ttgtgcggca actgcatgac gtccccgggc ggcacgtagt cgaacgtgtc gccgagcatc
     2161 ggcacgcccg cggcgtcgat ccgccagtag gcgatgtccc aatcgcccat gaccacggcg
     2221 aggccgaccg cgaagcagcc gccctccgcg tcggcggcga gcgcccgcac gacctggccc
     2281 ggggtgccct caagctcgat cccggtcgcg tgcccgtccg cgtccagcag gg
//
LOCUS       DASWNJ010000118         1629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_213873, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000118 DASWNJ010000000
VERSION     DASWNJ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213873"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VGB85_02135"
     CDS             <1..281
                     /locus_tag="VGB85_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132840.1"
                     /translation="RPPLLRLAAVLRAQGGLALCADVVRAYDGYLAALADPGIRRDLD
                     RVDYATRTHDPLFQRMVAGADRFDLAAQAVAGHLWVHCNEHLVRYGLL"
     gene            300..845
                     /locus_tag="VGB85_02140"
     CDS             300..845
                     /locus_tag="VGB85_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY0132841.1"
                     /translation="MTDLSHETACLPGQVVVFDLIGVLARPSWRELCVAPDLERWRRL
                     KVGALPESALWTAGQGAAYRAALGLRSDRVALLTRLRARGLTIVIASNFAREWAPTIR
                     AAMPDGLVARWLISGELGVAKPDPGFWAELLRHVAPGTLVVDDQRRNCEAATAAGLRG
                     LWAPAGAGFAARVLAALDAAP"
     gene            885..1253
                     /locus_tag="VGB85_02145"
     CDS             885..1253
                     /locus_tag="VGB85_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132842.1"
                     /translation="MSIQSPLRPGLAALASFASLVLGVVLGIACAPPVPGGACRGNPF
                     CDTGDLGAFCENDGQCRSGHCCENDDCDGGMCSLPCGKKQSCPAGLTCHDGNCHFRCD
                     IDLDCADGQRCKDDNFCSWD"
     gene            1308..>1629
                     /gene="glmM"
                     /locus_tag="VGB85_02150"
     CDS             1308..>1629
                     /gene="glmM"
                     /locus_tag="VGB85_02150"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977220.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY0132843.1"
                     /translation="MANERRLFGTDGIRGPANVPPMTPEVALRLGMAVAAHFLGCGRR
                     GSVVIGKDTRLSGYMFETALAAGVCAMGADVMLVGPLPTPGIAYITRSMRADAGVVIS
                     ASHNP"
BASE COUNT          187 a          636 c          550 g          256 t
ORIGIN      
        1 cgcgcccgcc gctgctgcgc ctcgccgcgg tgctgcgcgc ccagggcggt ctggctctgt
       61 gcgccgacgt cgtccgcgcc tacgacggtt acctcgccgc gctcgccgac cccgggatcc
      121 gccgcgacct cgaccgcgtc gactacgcga cgcgtacgca cgacccgctg ttccagcgca
      181 tggtcgccgg cgccgaccgc ttcgacctcg ccgcgcaggc ggtcgccggc cacctgtggg
      241 tccactgcaa cgagcacctc gtgcgctacg gcctgctgtg atcgacctgt gactgacctg
      301 tgactgacct gtctcatgag acagcctgtc tcccgggcca ggtcgtcgtc ttcgacctga
      361 tcggcgtgct cgcgcgaccc tcatggcgcg agctgtgcgt cgccccggac ctcgagcgct
      421 ggcgccggct caaggtcggt gcgctccccg agagcgcgct gtggaccgcg gggcagggcg
      481 cggcctaccg ggctgctctc ggcctgcggt cggaccgcgt cgccttgctg acccgtctgc
      541 gcgcgcgcgg cctcacgatc gtgatcgcca gcaacttcgc ccgcgagtgg gcgccgacga
      601 tccgcgccgc gatgcccgac ggcctcgtcg cgcgatggct gatcagcggc gagctcggcg
      661 tcgccaagcc cgaccccggc ttctgggccg agctactccg ccacgtcgcg cccggcacgc
      721 tcgtcgtcga cgaccagcgc cgcaactgcg aggcggccac cgcggccggc ctgcgcggcc
      781 tgtgggcccc cgccggagcc ggcttcgccg cgcgggtcct cgcggccctc gacgctgcgc
      841 cgtgacgctt tacctgcgcg cccggcttct ttatcctccc ggcgatgtcc atccaaagcc
      901 cgctccgccc cggtcttgct gcgctcgcct cgttcgcatc gctggtgctc ggcgtcgtgc
      961 tcggcatcgc ctgcgccccg ccggtccccg gcggtgcgtg ccgcggcaac ccgttctgcg
     1021 acaccggcga cctcggggcc ttctgcgaga acgacggtca gtgccgcagc ggccactgct
     1081 gcgagaacga cgactgcgac ggcggcatgt gctccctgcc gtgcggcaag aagcagtcgt
     1141 gcccggccgg cctgacctgc cacgacggca actgccactt ccgctgcgac atcgacctcg
     1201 actgcgccga cggccagcgc tgcaaggacg acaacttctg ctcctgggat tgatcgccgg
     1261 tcggcccccc gatccatgcc gcagcgggct gtgctaaagg gtcggcgatg gcgaatgaac
     1321 ggcgattgtt cggcaccgat gggatccgcg gacccgccaa cgtcccgccg atgacccccg
     1381 aggtcgcgct gcgcctcggc atggccgtgg cggcgcactt cctgggctgt ggccgccgcg
     1441 gcagcgtggt gatcggcaag gacacgcggc tctccggtta catgttcgag acggcgctcg
     1501 ccgccggcgt gtgtgccatg ggggccgacg tcatgctcgt gggtcccctg ccgacgccgg
     1561 ggatcgccta catcacgcgg tcgatgcgag ccgacgccgg ggtcgtgatc tccgcgtcgc
     1621 acaacccct
//
LOCUS       DASWNJ010000119         1697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_108438, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000119 DASWNJ010000000
VERSION     DASWNJ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108438"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VGB85_02155"
     CDS             <1..495
                     /locus_tag="VGB85_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY0132844.1"
                     /translation="FRMYSYVADELPALLAAELPLRAGALGICGHSMGGHGAMVLALR
                     RPDRFRSVSALAPICNPGAVAWGRKAFTAYLGADEAAWRDYDSCELLRAGARFPGPVL
                     VDQGDADKFLADQLRPEALAQVCADTHQPLQLRTQRGHDHSYFFIASFIGDHIAHHSQ
                     QLGR"
     gene            604..>1697
                     /locus_tag="VGB85_02160"
     CDS             604..>1697
                     /locus_tag="VGB85_02160"
                     /inference="COORDINATES: protein motif:HMM:NF040941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-like YCDxxxxGGGW domain-containing
                     protein"
                     /protein_id="HEY0132845.1"
                     /translation="MKSLPLLVVLGGLSGCLESNINNTDKDASSSSSDATTSSSDTSS
                     TGEPETTTGDSTENPTTGIAMPVCGDGLKGDGEGCDDGEANAEDAACTPECELNVCGD
                     GYILAGMEGCDDGPQNADDAACTSSCKPATCGDGHVLASVEMCDDGTNDGSYGSCLAD
                     CSAQAAHCGDGIIDSPEEECDDPGDPACLSSCMFARSCLVIHESNPELQSGPRTIYPV
                     DPTKPVEVVCDMETDGGGYTFLKVDVDSPQNDLPYPASKAETICAQYGMHLFVPRSAA
                     HLASAYGFSTVDNVLPVGGGSKGSSVDYLQILGIYPVTPGKSCLADALNPTECPQWAP
                     LDGEVWYVSDVSKNPSEPDPDSACKGCSMV"
BASE COUNT          278 a          640 c          547 g          232 t
ORIGIN      
        1 tttcgcatgt acagctacgt cgccgacgag ctgcccgcgc tgctggccgc cgagctgccg
       61 ctgcgcgccg gcgcgctggg gatctgcggg cattcgatgg gcggtcacgg ggcgatggtg
      121 ctcgcgctgc gccggcccga ccgcttccgc tcggtgtccg ccctcgcgcc gatctgcaac
      181 cccggcgcgg tcgcgtgggg tcgcaaggcg ttcaccgcgt acctcggcgc cgacgaggcc
      241 gcctggcgcg attatgacag ctgtgaattg ctgcgcgccg gcgcgcgctt ccctggcccc
      301 gtgctcgtcg accagggcga cgcggacaag ttcctcgccg accagctgcg ccccgaggcg
      361 ctcgcgcagg tctgcgcgga cacgcaccag ccgctgcagc tgaggaccca gcgcggccac
      421 gaccacagct acttcttcat cgccagcttc atcggcgacc acatcgcgca ccattcccag
      481 cagctcggcc ggtgattgaa atggcgccca cgcccgcgcg gacaggcgtt gcaggccccg
      541 tcgatccgtg ggaacaagga cggcggacct gcgccgttcg ccccgatgac gatgtcttac
      601 cgcgtgaagt ccctgcccct gctcgtcgtg ctcggcggcc tctccggctg cctcgagtcg
      661 aacatcaata acaccgataa ggacgccagc tcgagcagca gcgacgcgac gacgagctcg
      721 tccgacacca gctcgaccgg cgaaccggag accacgaccg gcgacagcac cgaaaacccg
      781 accaccggca tcgcgatgcc cgtgtgtggc gacggcctca aaggtgacgg cgagggctgc
      841 gacgacggcg aggccaacgc cgaggacgcc gcctgcaccc ccgagtgcga gctcaacgtc
      901 tgcggcgacg gttacatcct cgccggcatg gagggctgcg acgacggccc gcagaacgcc
      961 gacgacgccg cgtgcacctc gagctgcaag ccggccacct gcggcgacgg ccacgtcctg
     1021 gcgagcgtcg agatgtgcga cgacggcacg aacgacggat cttacggcag ctgcctcgcg
     1081 gattgctcgg cccaggccgc ccactgcggc gacggcatca tcgacagccc ggaggaggag
     1141 tgcgacgacc ccggggaccc ggcgtgcctg tcgtcctgca tgttcgcgcg cagctgcctg
     1201 gtgatccacg agtccaaccc ggagctccag tccggcccgc gcacgatcta cccggtcgac
     1261 ccgacgaagc ccgtcgaggt cgtctgcgac atggagaccg acggcggcgg ctacaccttt
     1321 ttgaaggtcg acgtcgactc gccgcaaaac gacctgccct accccgcgag caaggccgag
     1381 acgatctgcg cccagtacgg catgcacctg ttcgtgccgc gcagcgcggc ccacctcgcc
     1441 agcgcctacg gcttctccac cgtcgacaat gtcctgcccg tcggcggcgg cagcaagggc
     1501 agcagcgtcg actacctgca gatcctcggc atctatcccg tgacccccgg caagagctgc
     1561 ctcgccgacg cgctcaaccc caccgagtgc ccgcagtggg ccccgctcga cggcgaggtc
     1621 tggtacgtct cggacgtctc caagaacccc agcgagcccg accccgacag cgcatgcaag
     1681 ggctgctcga tggtcta
//
LOCUS       DASWNJ010000120         2845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_567615, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000120 DASWNJ010000000
VERSION     DASWNJ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567615"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VGB85_02165"
     CDS             complement(<1..641)
                     /locus_tag="VGB85_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132846.1"
                     /translation="MACRAFILALLSLGLVGCGGGEAAETATETTVDTGGVTDDPTGP
                     NMCAAEGAGCGAMAHCDPVDGNCYCDPGAYGDPATGCSPHGDLCGEAAGRVDHRVCLH
                     EVTNALDWEGITLGTSKRNDVRKVGKYLVPAHGEAPLPTLFNDSNYYRLHFCMLSEGF
                     EPLFPNWTHPLYNNFVYFRKNRQMYAGSLLEFSGDDLPVRYGFTVQTPEGSDEL"
     gene            complement(1142..1531)
                     /locus_tag="VGB85_02170"
     CDS             complement(1142..1531)
                     /locus_tag="VGB85_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132847.1"
                     /translation="MAKNLALLALILGIVSLFLPTTQGMGGAITVGVFTGTEKNPAGA
                     LLMAPPTFAALFGATLGRRRFGRGLGALHLIFGILGLLMCSIILGDERVQSGMVEAGM
                     GTYLAAAAGLLALIAGLIGLIKPDPQR"
     gene            complement(1593..>2845)
                     /locus_tag="VGB85_02175"
     CDS             complement(1593..>2845)
                     /locus_tag="VGB85_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132848.1"
                     /translation="VPAAPAGPPGPAYFAVDRKGVVRLDHGKLTLLANSPDTLIKDLQ
                     LGGDGAVWVVGYEDVLRLEGDKFRRVTRGGFSDLGASIDHFAVTAEGHVWAATFKGVS
                     HFDGKAWTTEHKAKIGAGEGVLDGIAVDRAGKVFVASTHGVHVRDGGVWKDIDLTRAG
                     ARDQLFLEDVALAPDGAVYVLASALLMRVAPGSDTPVKVPLGAGSFVSYNGLDVASNG
                     SLAVRDGDNVVAYPAGGDPRGYDGRKGADFRADNIRAVAADDTGRVWVASALGVAILG
                     PGDAKTEWPAGSMPELIGEVEDILVVGSGPATLPAAGPVQKGGLTGKVLRDGSPLAGV
                     SVELCPSPSMMFIESPCDSAGIKFAATADENGVWTFQDVPLGTYGLAVEVDGKWQITL
                     GERVGIGMKAGKVHDTGSMRLDRK"
BASE COUNT          404 a         1010 c         1015 g          416 t
ORIGIN      
        1 agctcgtcgc tgccctcggg cgtctggacc gtgaagccgt agcgcaccgg caagtcgtcg
       61 ccgctaaact cgagcagcga gcccgcgtac atctggcggt tcttccggaa gtacacgaag
      121 ttgttgtaga gcgggtgcgt ccagttgggg aacagcggct cgaagccctc gctcagcatg
      181 caaaagtgca ggcgatagta attgctgtcg ttgaagaggg tcggcagcgg ggcctcgccg
      241 tgcgcgggca cgaggtactt cccgaccttg cgcacgtcgt tgcgcttcga ggtgccgagc
      301 gtgatgccct cccagtcgag cgcattggtg acctcgtgca ggcacacgcg atgatcgacg
      361 cgcccggccg cctcgccgca caggtctccg tgcgggctgc acccggtcgc cggatctccg
      421 tacgcgccgg gatcgcagta gcagttgccg tcgaccgggt cacagtgggc catcgccccg
      481 cagccggcgc cctccgccgc gcacatgttc gggcccgtcg ggtcgtccgt gacgccgccg
      541 gtgtccaccg tcgtctccgt cgccgtctcc gcggcctctc ctcccccaca acccacgaga
      601 cccagcgaca gcagcgccag gatgaacgca cgacaagcca tgcgccgagg atggcggagc
      661 gcggcggacc gcgtcaacgc ggccgacgtg agcatcatcg tcgccgcgag atcgcggtcc
      721 cgcgcgtccg ccgttgcgcg cgacaagttt ttgggctgcc gcggccgccc gcgacacaat
      781 gtccgtcgcg cggtctgccg gacccgcagc gaggccgtga agatcgctgg cacccgcgtt
      841 gcagagcagc gaacatgctc gctcgcaaca ccccgtcacc gcgcccgtcc gcagcgcttc
      901 atctgcggtg ggccctgctc tccctcgtgc tcggctggtc ggcgaccacc agcgagtggg
      961 tccgcgtcga caatgggatc ccgctcatgc ggctgtccgc cagcctgggc ctgctgtgcg
     1021 gcgtcctggc gctgctgcct ccggggcccc gcagggtcag ggcaccccgg gcgggacgcg
     1081 gcctgcgggt ggcgctggtc gtgcagctct cgtgtgggct cgcgccgtgg ctgtccgagc
     1141 ctcagcgctg cgggtcgggc ttgatgaggc cgatgaggcc ggcgatcagc gcgagcaggc
     1201 cggcggcggc ggcgaggtag gtgcccatgc cggcctcgac catgccgctc tgcacgcgct
     1261 cgtcgccgag gatgatcgag cacatcagca ggccgaggat gccgaagatc aggtgcaggg
     1321 cgccgaggcc acgaccgaag cgacggcggc cgagcgtcgc gccgaacagc gcggcgaagg
     1381 tcggcggggc catcagcagc gcgcccgcgg ggttcttctc ggtgccggtg aacacgccga
     1441 cggtgatcgc cccgcccatg ccctgcgtgg tcgggaggaa cagcgagacg atgccgagga
     1501 tcagggcgag gagtgcgagg ttcttggcca tggtccgtga gtatcacgat cgcggccgca
     1561 gccgcgagcg acccggcgcg aacgaacgat gttcacttgc gatcgaggcg catcgagccg
     1621 gtgtcgtgga ccttgcccgc cttcatcccg atgccgacgc gctcaccgag cgtgatctgc
     1681 cacttgccgt cgacctcgac cgcgaggccg taggtgccga gcggcacgtc ctgaaacgtc
     1741 cacacgccgt tttcgtcggc cgtggcggcg aacttgatcc ccgcgctgtc gcatggcgac
     1801 tcgatgaaca tcatactcgg gctcgggcac agctcgacgg agacgccggc gagcgggctg
     1861 ccgtcgcgca gcaccttgcc ggtcaggccg cccttttgga ccgggccggc cgcggggagc
     1921 gtcgcggggc cggagccgac gacgaggatg tcctcgacct cgccgatcag ctcgggcatc
     1981 gagcccgcgg gccactcggt cttcgcgtcg ccggggccga gtatcgcgac gccgagtgcc
     2041 gacgcgaccc acacgcgccc ggtgtcgtcc gccgcgaccg cgcggatgtt gtcggcgcga
     2101 aagtcggcgc ccttgcgccc gtcgtatccg cgcgggtcac cgccggccgg ataggcgacg
     2161 acgttgtcgc cgtcgcggac cgcgaggctg ccgttgctcg cgacgtcgag gccgttgtag
     2221 gacacgaagc tgccggcgcc gagcggcacc ttcacgggcg tgtcgctacc cggtgcgacc
     2281 cgcatcagca gcgccgaggc gagcacgtac acggcgccgt caggcgcgag cgcgacgtcc
     2341 tcgaggaaca gctggtcgcg cgcgccggcg cgggtcaggt cgatgtcctt ccagacgccg
     2401 ccgtcgcgca cgtgcacgcc gtgggtcgag gcgacgaaca ccttgccggc gcggtcgacc
     2461 gcgatgccgt cgagcacgcc ctcacccgcg ccgatcttcg ccttgtgctc ggtggtccag
     2521 gccttgccgt cgaagtgcga gacgcccttg aaggtcgcgg cccacacgtg gccctctgcg
     2581 gtgacggcga agtggtcgat cgacgcgccg aggtccgaga agccgccgcg ggtgacccgg
     2641 cggaacttgt cgccctcgag gcgcagcacg tcctcgtaac cgaccaccca caccgccccg
     2701 tcgccgccga gctgcaggtc tttaataagg gtgtcgggcg agttggcgag cagggtgagc
     2761 ttgccgtgat cgagccgcac gacgcccttg cgatccaccg cgaagtaggc cggaccgggc
     2821 gggcccgcgg gcgcggccgg gaccg
//
LOCUS       DASWNJ010000121         1627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_673344, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000121 DASWNJ010000000
VERSION     DASWNJ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673344"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VGB85_02180"
     CDS             complement(<1..421)
                     /locus_tag="VGB85_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132849.1"
                     /translation="MRNTQVPELLAGTRIEGNGTAFAVAGAEPGMLLISTKRPVARWS
                     ELSATERSDVWALVDALTVTPARVGFDTEAPDGVPWHVCLRRASEDAFTTLPEFVGGE
                     EQRLLPALQTALALADEADLLSAFIQTSGVALLRDDLE"
     gene            460..>1627
                     /gene="dpdA"
                     /locus_tag="VGB85_02185"
     CDS             460..>1627
                     /gene="dpdA"
                     /locus_tag="VGB85_02185"
                     /inference="COORDINATES: protein motif:HMM:NF041059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase DpdA"
                     /protein_id="HEY0132850.1"
                     /translation="MKYFLPDSQDLVDPGFDFATEVRSGDRMRHRDDRYAHEIFSARA
                     FDGILVSKAIVDGTGSAGQARYSRVSRSRLLREGVRAFFRAGQHPWGPLDFMGDCGAF
                     TYVEQEAPPYTVDEVITFYDSCGFDIGVAVDHVIVEYRPQWDSGGVPASVKARQQLTL
                     ELAHEFFATITRGRLRLAPVGVAQGWSPRSYAAATAALQKIGYRYIALGGVASLRTVD
                     ILECLAAVDAIRGPDTRLHLLGVSRLAPLASFARYGVVSFDSTSPLRQAFMDAKHNYY
                     TIDRSYSAVRVPQVRGNVRLAARIRAGEVDAEVARAAEQRCLRALAEHAADNRRSIRP
                     VLDALRSYELLHDPRSDRTEVYREVLEAAPWRHCPCEVCRQLGHHIILFRGAERN"
BASE COUNT          250 a          587 c          525 g          265 t
ORIGIN      
        1 cctcgaggtc gtcacgcagc aacgcgacgc ccgaggtctg gatgaacgcg gacagcagat
       61 ccgcctcgtc cgcgagggcg agcgccgtct gcagcgccgg gaggagcctc tgctcctcgc
      121 cgccgacgaa ctcggggagc gtcgtaaacg cgtcctcgct ggcgcgccgc aggcacacat
      181 gccagggcac gccgtccggc gcctccgtat caaagccgac gcgcgcgggg gtcacggtca
      241 gcgcatcgac cagcgcccac acgtcgctcc gctccgtcgc cgacagctcc gaccagcggg
      301 cgaccgggcg cttcgtcgag atcagcagca tcccgggctc ggcgcccgcc actgcgaagg
      361 cggtgccgtt gccctcgatc ctcgtaccgg cgagcagctc cggaacctgg gtgtttcgca
      421 ccgctttacc ctaccccttc cgacccgcac tggcagccca tgaaatactt cctgccggac
      481 agccaggacc tcgtcgatcc cggcttcgac ttcgccacag aggttcgcag cggtgatcgg
      541 atgcgtcacc gggacgaccg ttacgcccac gagatctttt cagcccgggc cttcgacggg
      601 atcctcgtgt cgaaggcgat cgtcgacggt accggcagcg caggtcaggc tcgatactcc
      661 agggtttcgc ggtcccggct gttacgcgag ggcgtccgcg cgttcttccg cgccgggcag
      721 catccctggg gtccgctcga cttcatgggc gactgcggcg cgttcaccta cgtcgagcag
      781 gaggcgccgc cttacacggt cgacgaggtg atcacattct acgattcatg tgggttcgac
      841 atcggcgtcg ccgtcgatca tgtcatcgtc gagtaccgcc cgcagtggga cagtggtggc
      901 gtgcccgcgt cggtcaaggc gcgtcagcag ctcacgctgg agctcgctca tgagttcttc
      961 gccacgatca cgcgtggcag gctcaggctg gcgcccgtcg gcgtcgcgca gggctggagc
     1021 ccacgatcgt acgctgccgc caccgccgcc ctgcaaaaga tcggctatcg atatatcgcg
     1081 ctgggcgggg tggcgtcgct tcggactgtc gacattctcg agtgcctcgc tgccgtcgac
     1141 gcgatccggg gccccgatac ccgcctgcac ctgctcggtg tgtcacgcct cgccccactg
     1201 gcgagcttcg cccggtacgg cgtggtcagc ttcgacagca cctcgccgct gcggcaggcc
     1261 ttcatggacg cgaagcacaa ttactacacg atcgatcgca gctatagcgc cgtccgggtc
     1321 ccacaggtcc gcgggaacgt ccgcctggcg gcccgcatcc gcgccggaga ggtcgacgcg
     1381 gaggtggccc gcgcggccga acagcgctgc ttgcgagccc tcgccgagca cgccgccgac
     1441 aatcgacgct cgatccgtcc ggtgctcgac gcgctgcgca gctacgagct gctccacgac
     1501 ccgcgcagcg atcgaaccga ggtctatcgt gaggtcctcg aggcggcgcc gtggagacac
     1561 tgtccatgcg aggtgtgcag gcaactcggt caccacatca tcctgtttcg cggcgccgag
     1621 cgaaacc
//
LOCUS       DASWNJ010000122         6070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_284902, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000122 DASWNJ010000000
VERSION     DASWNJ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6070
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284902"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            888..1637
                     /locus_tag="VGB85_02190"
     CDS             888..1637
                     /locus_tag="VGB85_02190"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0132851.1"
                     /translation="MARPVALRLDGVTKRHGVGEDAVVALDTMSLSVTAGEFVCVVGA
                     SGCGKSTMLNLVAGLDTPSAGQVQVQGGRPALMFQESALFPWLTVEENVAFPLQMQGV
                     PRGERKQRVAELLAMVHLEKFGRRRPHELSGGMKQRVALARALAQRANVLLMDEPFAA
                     LDAMMRDHLHCELETLWQQTGLTVVFVTHNVREAVRLGDRVVLLSSRPGRFLAEYAID
                     IERPRRIESPEVARTAREITDRLHEAARSAA"
     gene            1627..2595
                     /locus_tag="VGB85_02195"
     CDS             1627..2595
                     /locus_tag="VGB85_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0132852.1"
                     /translation="MLPETLTGPARAHLHAIDGEAEVNEDSHTRASLHAVATPTPSAP
                     VPADSFETLAAGPERSRVGDAWSVVWPKLAAVGMALAIWELVVLSGWRPDYVLPGPGA
                     VLGRLFADIASGEVLVAAAITMKRAVIGYAIALIAGVAIGLSMARVKVLRTAAGSLLT
                     GLQTMPSVAWFPLAILLFQLSEAAILAVVVLGAAPSIANGLLHSFDQVPPLLVRAGRV
                     LGARGLGLYRHIILPAALPGFLTGLKQGWAFAWRSLMAGELLVIIGHAPSIGVRLQYS
                     RELSDAEGLLAWMIVVMILGVLIDGVVFGSLERAVHRRRGLTGVHA"
     gene            2653..3591
                     /locus_tag="VGB85_02200"
     CDS             2653..3591
                     /locus_tag="VGB85_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132853.1"
                     /translation="MPSLEHEALVEMFRTHPELVPLLLREVFGVEVPAYERLVVSEAV
                     LDQLTPTEFRADLVVDLFDGADKRPRMSGVVEVQLRIDDDKLYSWPAYLILSRSRRRC
                     EAFVLVVAPDPAVAAWARRPIHLGPGNELRVFVLGPAEIPTITDPDVAARYPELTVLS
                     ALAHGNEPEHGMPVLRVMLGALRTLDRKEGEVYLRIVYKTLAARVRHAFQEEVMLNEP
                     SPDVEVERESKELFEVLQRIWARDRAAKASAEAAGEARGFVVGQARALLKILDRAGIA
                     LSPEHHDKITSCEDPAQLDAWIDRAFDAKSAADLFG"
     gene            3666..3995
                     /locus_tag="VGB85_02205"
     CDS             3666..3995
                     /locus_tag="VGB85_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132854.1"
                     /translation="MYLDANFRSLRVSGTTSEGVPVIAGLFPVVNSEGVALDMLLELF
                     HDRRWSVDWFDFLVHARDFGWNLRTTLGKIHTALEAVEGPAFAGAWLRSAEAMVPTLL
                     RVRVVSA"
     gene            complement(4029..4919)
                     /locus_tag="VGB85_02210"
     CDS             complement(4029..4919)
                     /locus_tag="VGB85_02210"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY0132855.1"
                     /translation="MQLSELLERLFATLRRWFEAFGEILPNLVVAVLVLLIFGYGSRY
                     VGRTVCRLMSRVTTHATLSDLLGTIARVTTIICGLLIALGLLQLDKTVTSLLAGVGVV
                     GLALGFAFQDIAANFMSGFIMALRPPFEKGDLVDTGGQVGIIERIELRATLLTTLQGL
                     SVIIPNKDVFQSPIINYTRTGDRRMDLLVGIEQSAPLELAQSLAVAAARELKHRNPAR
                     EPEVFFTEFIEDSVLFTVRVWLASSDERTFLEAKSAAIIGIKQAFDAHEIAIFLPGGE
                     LGAQDRKDRKSRGSKPARKR"
     gene            complement(4968..5777)
                     /locus_tag="VGB85_02215"
     CDS             complement(4968..5777)
                     /locus_tag="VGB85_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132856.1"
                     /translation="MTTTLIATLTLGLLQAPPAQPARTQTPVAPVTTPAPAPAPEPAP
                     TPAPEPAGSVDPFAAPAEPAPAPAPEPTQIGVLYTPDTAPLVPPPSPVAAEQTDALKY
                     RRLVFSNFYTLNMGMYPVPSGELSLFLGTTLRPRKSTLGTDWNTAIGYQLTMSLGYAD
                     YWFSESQPIQDRISDEFFGPDPIFFHRHALLAQGHGGRNGRLYYAMGGGAVMWHTLLI
                     GVEAEGKIGYIFSARENSRTKGIVGGQLRLGGPFDHFPLPQFGAFVGFMVF"
BASE COUNT          817 a         1963 c         2308 g          982 t
ORIGIN      
        1 cgtcgtggtg tcgtcgccgg ccgtggtggt gccgccggcc gtggtgtcgc cgccggccgt
       61 ggtggtgccg ccggtcgtgg tgtcggtcgt gctgggcagt ggtgcgccgg tcgaggtcgg
      121 gtcgccgctc gtcgccgcgc cggtgtccga ggcgctcgca ggcatgttcg tcgtcgacga
      181 gctgccggtc ggtgcgtcgc cggtgtcctc gccggcggcg ggggcgggca ggcggcgagc
      241 agtagcgcga agccggtcgg caggcgcgag cggagtgcgg cggttggcga ggacatctgc
      301 caatgacgat ggcccgcccg ccgcgcgatg tacagtgatc aaaccgcgct ctcggagcgc
      361 gagggcctcg acgatcttgt gtcatcggtg gcgggtgagc gccagttgcc gcgcgtgcgc
      421 tcgacggcga tgatgtatag aaataggcca tcgcagctga tggcgccgtg gtggaggtat
      481 tgcggggcgt cgaagcggct gtgtaatgcg atgacggtac gaatgtagca ctgtctgtcg
      541 ccctcgcatg cagcgctcct ctgagggtcg tggcccgtcg cgtccgtgct ctcgcgcgat
      601 gggcgcacgt ccagcgcagt ggacaaggtg gactgtcgag cggccgcaga cgccgcgaac
      661 gcccgcgaga acgcgcctgg agaccggtta tctggcccgc gcgccggttc cggtcgcgtg
      721 cgcggtgttc gccgtattga cggtcattcc atcatactgt acgcctacgg tgtcctcccg
      781 ggagacttgc catgagcgtt gccctgcctc acgcccccaa gctgcgcacg tccgtcccgg
      841 ccgctgcttt cgcgtccgtc ccggccgctg ctttcacgtc cccggcggtg gcccgtccgg
      901 tcgcgctgcg cctcgacggt gtcaccaagc ggcacggcgt cggtgaggac gcggtggtcg
      961 ccctcgacac catgtcgctg agcgtcaccg ccggcgagtt cgtgtgcgtg gtcggggcgt
     1021 cgggctgcgg caagtcgacg atgctcaacc tcgtcgccgg cctcgacacg ccgtcggccg
     1081 ggcaggtgca ggtgcagggc gggcgcccgg cgctgatgtt ccaggagtcc gcgctgttcc
     1141 cgtggctgac ggtcgaggag aacgtcgcgt tcccgctgca gatgcagggc gtgccgcgag
     1201 gcgagcgcaa gcagcgggtc gccgagctgc tggcgatggt gcacctcgag aagttcggtc
     1261 gtcgccggcc gcacgagctg tcgggcggca tgaagcagcg cgtcgcgctg gcgcgggcgc
     1321 tggcgcagcg ggccaacgtc ctgctgatgg acgagccgtt cgcggcgctc gacgcgatga
     1381 tgcgcgacca cctgcactgc gagctcgaga cgctgtggca gcagaccggg ctgacggtcg
     1441 tgttcgtgac ccacaacgtg cgcgaggccg tgcgcctcgg cgaccgcgtc gtgctgctgt
     1501 cgagccgccc cggccggttc ctggccgagt acgcgatcga catcgagcgc ccgcgccgga
     1561 tcgagtcgcc cgaggtcgcc cgcaccgcgc gcgagatcac cgaccgcctg cacgaggccg
     1621 cgcgcagtgc tgcctgagac gctgaccggg cccgcgcgcg cgcacctgca tgcgatcgac
     1681 ggcgaggccg aggtgaacga ggacagccac acccgcgcct ccctgcacgc cgtggcgacc
     1741 ccgacgccga gcgcacctgt ccccgcggac agcttcgaga cgctggccgc cggccccgag
     1801 cgctcgcgcg tcggcgacgc gtggtcggtg gtatggccca agctggccgc ggtcggcatg
     1861 gcgctggcga tctgggagct ggtggtgctc agcggctggc ggcccgacta cgtgctgccg
     1921 gggcccggcg cggtgctcgg gcgcctgttc gccgacatcg cctcgggcga ggtgctggtc
     1981 gccgccgcga tcacgatgaa gcgcgccgtg atcggctacg cgatcgcgtt gatcgccggc
     2041 gtggcgatcg gcctgtcgat ggcccgcgtc aaggtgctgc gcacggcggc cggctcgctg
     2101 ctgaccggcc tgcagacgat gccctcggtc gcatggttcc cgctggcgat cttgctgttc
     2161 cagctcagcg aggcggcgat cctcgcggtc gtggtgctcg gcgcggcgcc gtcgatcgcc
     2221 aatggcctgc tgcacagctt cgaccaggtc ccgccgctgc tggtccgcgc cggccgggtg
     2281 ctcggcgccc gcggcctcgg cctgtatcgc cacatcatcc tgccggccgc gctgccgggg
     2341 ttcctgaccg gcctcaagca gggctgggcg ttcgcgtggc gcagcctgat ggccggcgag
     2401 ctgctcgtca tcatcggcca cgccccgtcg atcggcgtgc gcctccagta ctcacgcgag
     2461 ctcagcgacg ccgagggcct gctcgcgtgg atgatcgtgg tgatgatcct cggcgtgctg
     2521 atcgatgggg tcgtgttcgg ctcgctcgag cgcgcagtcc atcgccgccg cgggctcacc
     2581 ggcgtgcacg cctgacgggc gcgtttacgc gcgaaacttg gtcgacgcgc gttagggtgg
     2641 tagcagggcg gcatgccctc gctcgagcac gaagcgctgg tcgagatgtt tcggacgcat
     2701 cccgagctgg tgccgctgtt gctgcgcgag gtgttcggcg tcgaggtccc ggcgtacgag
     2761 cggctcgtcg tcagtgaggc ggtcctcgac cagctgacac cgacggagtt tcgtgccgac
     2821 ctcgtcgtcg atctgttcga cggcgccgac aagcgaccgc gcatgagcgg tgtggtcgag
     2881 gtccagctgc gcatcgacga cgacaagctc tacagctggc cggcctacct gatcctgagc
     2941 cgctcgcgtc gccgctgcga ggccttcgtc ctcgtcgtcg cgcccgaccc cgcggtggcg
     3001 gcgtgggccc gtcgaccgat ccacctcggc cccggcaacg agctgcgggt gttcgtgctc
     3061 ggcccggcgg agatcccgac catcaccgat ccggacgtgg cagcccgtta tcccgagctg
     3121 accgtgctct cggcgctggc ccacggcaac gagccggaac acggtatgcc ggtgctccgg
     3181 gtgatgctgg gcgcgctgcg tacgttggac aggaaggaag gcgaggttta tcttcggatc
     3241 gtctacaaga cactggccgc gcgcgtgcgc cacgccttcc aggaggaagt catgctcaac
     3301 gaaccatccc cggacgtaga ggtcgagcgc gagagcaagg agctcttcga ggtcttgcag
     3361 cggatctggg cgcgcgacag ggccgccaag gccagcgccg aggcagcggg tgaggcgcga
     3421 ggttttgtcg tgggccaggc tcgtgctttg ctcaagatcc tcgatcgtgc cggcatcgcg
     3481 ctgtctccgg agcatcacga caagatcacc tcctgcgagg atccggcgca gctcgacgcg
     3541 tggatcgatc gggcgttcga cgcgaagtcg gcggccgacc tgttcggctg agcgcgcccg
     3601 cgcgtgcccg cgcgtgcccg tgggtgcccc acggcggggg cggggttcga tagattggcg
     3661 gggtcatgta tctcgacgcc aactttcgca gcctgcgtgt ctccgggacc acctccgagg
     3721 gtgtgcctgt gatcgcgggg ctgtttccgg tggtcaacag cgagggtgtg gcgctcgaca
     3781 tgttgctcga gctgtttcac gaccggcgct ggtccgtcga ctggttcgac ttcctcgtgc
     3841 acgcgaggga cttcggctgg aacctgcgca cgacgctcgg caagatccac accgccctcg
     3901 aggcggtcga ggggccggcg ttcgcgggcg cgtggctgcg gtccgcggag gcgatggtgc
     3961 cgacgctgct gcgcgtccgc gtggtgtctg cctgagcgtg ttgcgatcca ggcgtcgtgg
     4021 gcgggccgtc aacgcttgcg cgccggcttg ctgccacgcg acttgcggtc cttgcgatcc
     4081 tgggcgccaa gttcgccgcc cgggaggaag atcgcgatct cgtgggcatc gaaggcctgc
     4141 ttgatcccga tgatcgcggc ggacttcgcc tcgaggaagg tgcgctcgtc ggaggaggcc
     4201 agccacacgc ggacggtgaa cagcaccgag tcctcgatga actcggtgaa gaagacctcg
     4261 ggctcgcgcg ccgggttgcg gtgcttgagc tcgcgtgcgg cggcgacggc gaggctctgg
     4321 gccagctcga gcggggcgct ctgttcaatg ccgacgagca ggtccatgcg acggtcgccg
     4381 gtgcgggtgt agttgatgat cgggctctgg aagacgtcct tgttggggat gatgacgctg
     4441 aggccctgaa gagtggtcag cagggtcgcg cgcagctcga tgcgctcgat gatgccgacc
     4501 tgtccgccgg tgtcgacgag gtcgcccttc tcgaaggggg ggcgcagggc catgatgaag
     4561 ccggacatga agttggcggc gatgtcctgg aaggcgaagc cgagggcgag gccgacgacg
     4621 ccgacgccgg cgagcagcga ggtgacggtc ttgtcgagct gcaggaggcc gagggcgatc
     4681 agcaggccgc agatgatcgt cgtgacgcgg gcgatcgtgc cgagcaggtc gctcagcgtc
     4741 gcgtgcgtgg tgacgcgcga catcagcctg cagacggtcc ggccgacgta gcggctgccg
     4801 tagccgaaga tcagcaggac gaggacggcg acgacgaggt tggggaggat ctccccgaac
     4861 gcctcgaacc agcgccggag ggtcgcgaac agccgttcga ggagctcgga gagctgcatg
     4921 cctcaggtgc gtagcacgcg atcggccggg cggcaggcgc cgggccgcta gaacaccatg
     4981 aagccgacga acgcgccgaa ttgcggcagc ggaaagtggt cgaaggggcc gccgaggcgc
     5041 agctgtccgc cgacgatgcc cttggtgcgc gagttctcgc gggccgaaaa gatgtagccg
     5101 atcttgccct cggcctcgac gccgatcagc agggtgtgcc acatgaccgc gccgccgccc
     5161 atcgcatagt agaggcggcc attgcggccg ccgtggccct gggccagcag cgcgtggcga
     5221 tgaaagaaga tcgggtccgg tccaaagaac tcgtcgctga tgcggtcctg gatcggctgg
     5281 gattcgctga accagtagtc ggcgtagccg aggctcatcg tcagctggta gccgatcgcg
     5341 gtgttccagt cggtccccag cgtgctcttg cgcgggcgca gggtggtgcc gaggaacagc
     5401 gagagctcgc ccgaggggac cgggtacatg cccatgttga gcgtgtagaa gttggagaac
     5461 acgagccggc gatacttgag cgcgtcggtc tgctcggccg cgacggggct cggcggcggc
     5521 accagcggcg ccgtgtccgg cgtgtacagg acgccgatct gggtcggctc gggcgcgggc
     5581 gccggggcgg gctcggccgg cgcggcgaac gggtcgacgg acccggcggg ctcgggcgca
     5641 ggcgtcgggg ccggctcggg cgccggggcg ggtgcaggcg tcgtcacggg cgcgaccggg
     5701 gtctgcgtgc gcgcgggttg agcggggggg gcttgcagca ggccgagagt gagcgtcgcg
     5761 attagagtag tggtcatatc cgtgcaggcc gcgagcgtgg cctcgccgcg aagcatcgct
     5821 cggtggcgag atctgctcaa gtacgcccgc ggaacgccgt ccctccgcga ctcgcgggag
     5881 atcgggcctg cgaggcccga gtgtacgagg ccgtcgtcgc ggggttcttc gcggtcagcc
     5941 ggcgctgagc accacgaccg ggcacggtgc gttcttgagc atgctctcga cgcggtgacc
     6001 gaagaaggcg cgggcgctga cggcccggag gctgctgccg atgacgatga tgtcgtagtt
     6061 accctcgcgg
//
LOCUS       DASWNJ010000123         1664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_108611, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000123 DASWNJ010000000
VERSION     DASWNJ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108611"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VGB85_02220"
     CDS             <1..750
                     /locus_tag="VGB85_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HEY0132857.1"
                     /translation="IIILIVVFHQELRRIFLRVGQRLLPHGRREATRSAVGELVLAID
                     RLRRAKVGALVILQGEIDVLEVVSDRGREINAALQADTIVALMIPHPINLTHDGALLI
                     QNFRIARAGVICPLTQRDRLDPSFGTRHRGGIGVSEETDALVAVVSEERGEIRVVHRG
                     EISESITAAELEARIAEWLDQPPEAAAMAASVVAGSRAAVASESLVLRSLSRTRVDLR
                     RESLRSGESLVDLSANPPAPRVRDAVAEGER"
     gene            756..>1664
                     /locus_tag="VGB85_02225"
     CDS             756..>1664
                     /locus_tag="VGB85_02225"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEY0132858.1"
                     /translation="MLAWIGRGIVSHWLTKLSALVIALVFFVVTRDDVTRTFTIPLHV
                     VPDPNRVLLTKLPEAVTVELHGSWARMSRLREQELGRAELNLSDARPGPLAIDPAAIV
                     MPEGVIFRQIHYEKVDLRFDRIIERAVLVKADLRGNVHPDFEQTGVTVEPPRWRIRGG
                     SGPVGEVQALQTEPIDLEGATGDLNVDVPLQNPRDDVDYTAVPPGELPRVKVTVAVRP
                     RASERKLSAAVTWPTDQVAPAEIPTQVDVIVRGGVPDLRVLDAALSPPLVAVARRDKP
                     TAEFPDGSVAFTVQFTEAVPPEVQGRL"
BASE COUNT          224 a          638 c          578 g          224 t
ORIGIN      
        1 atcatcatcc tgatcgtcgt gttccatcag gagctgcggc ggatcttcct ccgcgtcggc
       61 cagcgcctgc tgccgcacgg gcgccgcgag gcgacccgca gcgccgtcgg cgagctggtg
      121 ctggcgatcg accgcctgcg ccgggccaag gtcggcgcgc tcgtcatctt gcagggcgag
      181 atcgacgtgc tcgaggtcgt cagcgaccgc ggccgcgaga tcaacgcggc gctgcaggcc
      241 gacacgatcg tcgccctgat gatcccgcac ccgatcaacc tcacccacga cggcgcgctg
      301 ctgatccaga actttcgcat cgcccgcgcc ggcgtcatct gcccgctgac ccagcgcgac
      361 cgcctcgacc cgagcttcgg cacccgccac cgcggaggga tcggcgtcag cgaggagacc
      421 gacgcgctcg tcgccgtcgt cagcgaggag cgcggcgaga tccgcgtcgt gcaccgcggc
      481 gaaatttccg agtcgatcac cgccgccgag ctcgaggcgc ggatcgccga gtggctcgac
      541 cagccgcccg aggccgcggc gatggccgcc tccgtggtcg ccggttcccg ggccgcggtc
      601 gccagcgaga gcctggtcct gcgcagtctg tcgcgcacgc gcgtcgacct gcgccgcgag
      661 tcgctgcgat cgggcgagag cctcgtcgac ctgtccgcca acccgccggc gccccgcgtg
      721 cgtgacgcgg tcgcggaggg cgagcgatga ggcgggtgct ggcctggatc ggccgcggga
      781 tcgtcagcca ctggctgacc aagctcagcg cgctggtgat cgcgctggtg ttcttcgtcg
      841 tcacccgcga cgacgtcacg cgcaccttca cgatcccgct gcacgtcgtg cccgacccca
      901 accgcgtgct gctgaccaag ctgcccgagg ccgtcaccgt cgagctgcac ggctcgtggg
      961 cgcgcatgag ccggctgcga gagcaggagc tcggccgcgc cgagctcaac ctctccgacg
     1021 cgcgcccggg cccgctggcg atcgacccgg cggcgatcgt gatgcccgag ggcgtcatct
     1081 ttcgccagat ccactacgag aaggtcgacc tgcgcttcga tcgcatcatc gagcgcgccg
     1141 tgctcgtcaa ggccgacctg cgcggcaacg tgcacccgga cttcgagcag accggcgtca
     1201 ccgtcgagcc gccgcgctgg cggatccgcg gcggcagcgg tccggtcggc gaggttcagg
     1261 ccctgcagac cgagccgatc gacctcgagg gtgcgaccgg ggacctcaac gtcgacgtgc
     1321 cgctgcagaa cccgcgcgac gacgtcgact acaccgcggt gccccccggc gagctgccgc
     1381 gggtcaaggt caccgtcgcg gtgcggccgc gggccagcga gcgcaagctc agcgccgcgg
     1441 tcacctggcc cacggaccag gtcgcgccgg ccgagatccc gacgcaggtc gacgtcatcg
     1501 tccgcggcgg cgtgcccgac ctgcgggtcc tcgacgccgc gctgtcgccc ccgctggtcg
     1561 ccgtcgcccg gcgcgacaag ccgaccgccg agttccccga cggctcggtc gcgttcaccg
     1621 tgcagttcac cgaggccgtg ccgccggagg tccagggccg cctg
//
LOCUS       DASWNJ010000124         1829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_2489, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000124 DASWNJ010000000
VERSION     DASWNJ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2489"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VGB85_02230"
     CDS             <1..831
                     /locus_tag="VGB85_02230"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY0132859.1"
                     /translation="EIDETMTMDPADLRRKLSSGTDAKPRAICVVHLFGVAADMDPIL
                     ALAREHDIPVIEDCAQSCGAMYRGKTVGHLGAMGIFSFQLNKVITAGEGGALVTDDAA
                     LYDRAVRLHDHGNHRGDEAAGSPLIGEGLRVSELTAAILLAQLGRLDDILSRLRSAKR
                     YLLERLADVPGLTLTRIPDPEGDGGVSLTFFTESVEKTARVVEALNAEGIRALRQYGG
                     KLLYQQPAIRSANLQPEPLCPRSVDLVGRCVFLGLTTTLTKRDLQDIVTAIRKVMRHV
                     "
     gene            858..>1829
                     /locus_tag="VGB85_02235"
     CDS             858..>1829
                     /locus_tag="VGB85_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEY0132860.1"
                     /translation="MAAGAPHAGESSRAAIERDASDLRRSLRLNLLGYVVKLAHPFLL
                     IVVIARYGAGAYGVFALVQAVLVVLLRLALLGLDKGLLWWIPRQVQADERLGLRAVLI
                     IATLTSALLAAATALILAPLIAGWAGRPDITDSLRWMAVGLVPMALLEVLCQASVGKR
                     HLEAHVIAKEGVVAMTMVVAALLLHALGLVATGLALAFLISYVAGLLAVLWVFRRAFA
                     GSAWGRRTWRLPAELWRYSWPMWLSELANALFVRLDVFILAALTDEVAVGIFAGAAQY
                     AQNVTAIRVSFDPMVTAMVSQIGHANDRDRMRRGFAHAWLLVATLQIP"
BASE COUNT          232 a          678 c          639 g          280 t
ORIGIN      
        1 gagatcgacg agacgatgac gatggacccc gccgacctgc gccgcaagct gtcctcgggg
       61 acagacgcca agccgcgtgc gatctgcgtg gtccacctgt tcggcgtcgc cgccgacatg
      121 gacccgatcc tcgcgctcgc ccgcgagcac gacatccccg tgatcgagga ctgcgcgcag
      181 tcgtgcggcg cgatgtaccg cggcaagacc gtcggccacc tcggcgcgat gggcatcttc
      241 agcttccagc tcaacaaggt gatcaccgcg ggggagggcg gggcgctcgt caccgacgac
      301 gccgcgctgt acgaccgtgc cgtgcgcctg cacgaccacg gcaaccaccg cggcgacgag
      361 gccgccggct cgccgctgat cggcgagggc ctgcgggtca gtgagctgac cgccgcgatc
      421 ctgctcgcgc agctcggccg cctcgacgac atcctgtcgc gcctgcgcag cgccaagcgc
      481 tacctgctcg agcgcctcgc cgacgtgccg gggctgacgc tgacccgcat ccccgacccc
      541 gagggcgacg ggggcgtgtc gctgaccttc ttcaccgagt cggtcgagaa gaccgcgcgc
      601 gtggtcgagg cgctcaacgc cgagggcatc cgcgcgctgc gtcagtacgg cggcaagctg
      661 ctctaccagc agcccgcgat ccgcagcgcc aacctccagc ccgagccgct gtgcccgcgc
      721 tcggtcgacc tcgtcggccg ctgcgtgttc ctcggcctga ccaccacgct gaccaaacgc
      781 gatctgcagg acatcgtcac cgcgatccgc aaggtcatgc ggcacgtttg accgccgacg
      841 agggtcgcgc agacgcgatg gctgccgggg ccccccacgc gggtgagagc agccgcgccg
      901 cgatcgagcg cgacgccagc gacctgcgcc gcagcttgcg gctcaacctg ctcggctacg
      961 tcgtcaagct ggcccacccg ttcctgctga tcgtcgtgat cgcgcgctac ggcgcgggcg
     1021 cgtacggggt gttcgcgctg gtgcaggcgg tgctcgtcgt gctgctgcgg ctcgccctgc
     1081 tcggcctcga caaggggctg ctgtggtgga tcccgcgcca ggtgcaggcc gacgagcgtc
     1141 tcgggctgcg cgccgtgctg atcatcgcca cgctgacctc cgccctgctc gcggccgcga
     1201 cggcgctgat cctcgcgccg ctgatcgccg gctgggccgg gcgccccgac atcaccgaca
     1261 gcctgcgctg gatggcggtc ggcctggtcc cgatggccct gctcgaggtg ctgtgccagg
     1321 cgtccgtcgg caagcggcac ctcgaggccc acgtgatcgc caaggagggc gtcgtggcga
     1381 tgacgatggt cgtcgcggcg ctgctgctgc acgcgctcgg cctggtcgcc accggcctgg
     1441 cgctcgcctt cctgatctcg tacgtcgccg gcctgctggc ggtcctgtgg gtgtttcgtc
     1501 gcgccttcgc aggctcggcg tggggccggc ggacctggcg gctgcccgcg gagctgtggc
     1561 gctacagctg gccgatgtgg ctcagcgagc tggcgaacgc gctgttcgtc cgcctcgatg
     1621 tcttcatcct cgcggcgctg accgacgagg tcgcggtcgg catcttcgcg ggggccgccc
     1681 agtacgcgca gaacgtcacg gcgatccgcg tgtccttcga cccgatggtg acggcgatgg
     1741 tctcgcagat cggccacgcg aacgaccgcg accgtatgcg ccgcggcttc gcgcacgcct
     1801 ggctgctggt cgcgaccctg cagatcccg
//
LOCUS       DASWNJ010000125        13281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_2499, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000125 DASWNJ010000000
VERSION     DASWNJ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 13281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13281
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2499"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VGB85_02240"
     CDS             <1..538
                     /locus_tag="VGB85_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132861.1"
                     /translation="EKHTWRAWTTEHCVNFVEIPTEPYAFAQKTTRSVYNDDLLCAKF
                     WEELLAGGGIQAVEAEVLNGLDHLFNNGVSSPAMVGIPQPIKTEMYVWPGGWYYVRAG
                     ADISNQQITDWSREPLPGEENLMLVGEAYWPQRPGWSIGAYNSADKLLEAKFGIVRVL
                     DRQVRAGSPRKNRAQGGH"
     gene            621..1064
                     /gene="ruvX"
                     /locus_tag="VGB85_02245"
     CDS             621..1064
                     /gene="ruvX"
                     /locus_tag="VGB85_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEY0132862.1"
                     /translation="MRALGLDVGSKTIGVAVGDPSGTIASARTVLARRGHVADAAAIA
                     ELARTEAAGAVVVGLPLELDGTVGHRARLVQRFVDALAAVLKDMPNPVKLHTWDERFS
                     TAAAERTMIEADASRARRKQSIDAVAAQFILQGWLDAQRRREGLA"
     gene            1154..2320
                     /gene="mltG"
                     /locus_tag="VGB85_02250"
     CDS             1154..2320
                     /gene="mltG"
                     /locus_tag="VGB85_02250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY0132863.1"
                     /translation="MKKPRRRWLRRSRWSQYVALAVVATTLVVAALVGYRAYDQLVQY
                     PLRAGRGDATPVVLTVPRGASFPQVLGQLTEAGVVPADEALYFKLFVLHRGAASKVTA
                     GEHTFRGDMTPEEILAELVRAQRAEEIRVTIPEGRNILEAADILAAAGIGTVEQILAA
                     MRDPALLEELGITGPSVEGYLFPDTYKFPAKATPQQVLRRMVEQHRKVYAGLARGHRE
                     AIEELHKTLGWGDREIVIMASLVEKETAAKHERPLIAGVFLNRLRFQSFQPKRLETDP
                     TIIYGCTAVVEKSEACKQFTDRIRRIHLRDTENPYNTYTHEGLPPGPITNPGKAALEA
                     VLAPKKSRFLYFVSRNDRTHQFSKSIAEHEAAVEKYMRQGAVGDGSAAGAAAAD"
     gene            complement(2334..2930)
                     /locus_tag="VGB85_02255"
     CDS             complement(2334..2930)
                     /locus_tag="VGB85_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132864.1"
                     /translation="MHAEAVAELADGVRGRARIVVAVASGDRPARVEVWDFSQNNERG
                     LLARIGEPEVLLDLGDVRGRLVTGAVATLRREMASPGSETVYPIGLEGSPEEVLAELA
                     RLATASTAAPDAATRARSLARFIRGIDDSLLWDEGRLPELLRRLQNAPWTIVAQSQQG
                     ARRSISATEEARPVQLLMTRNHGRWVLFTVSDGPPSGP"
     gene            3258..4364
                     /locus_tag="VGB85_02260"
     CDS             3258..4364
                     /locus_tag="VGB85_02260"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEY0132865.1"
                     /translation="MLATSVVFSSSGRGVAEAAMFAPAAAGASAASGSGAKLAILPLT
                     IEGGLGEADQAELTKALSAGLQRGNFSIVSPEQVLATDARAGSCDNTVCYQNIARRTG
                     AAYVVRAVVSVKDRDYTVKVELVGADGKRLAATQDGCEICGVVDATGLIDSAAATLRL
                     KLDALTKGPAALKLATDPAGAIVTIDGEVVGEAPLERPIVPGKHLIRVSEEGFISIER
                     EVTFVEGVSEDLTFKLEKLPSRLPGRRFGYVSLGFGLAALGGAIGFAILDDKPYKLGG
                     NCSTTYGGDPDAPFNNIPGQDSITGRDECAKLWRTEPFSIPLAIAGGALLTLGIAILL
                     NSRKRTQAEKGPTARRGMPRFGVGPGSVSVSGRF"
     gene            complement(4368..5573)
                     /locus_tag="VGB85_02265"
     CDS             complement(4368..5573)
                     /locus_tag="VGB85_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132866.1"
                     /translation="MCDTTVIVRPGQVLFAKSSDRDLGEAQLLEWHPRRQHAPGAALA
                     CTWISVPQVAETAAVLLSRPFWTWGAEMGTNEHGVVIGNEAVFTRSPLARVGLTGMDL
                     VRLALERSDSAEAACEVIVALLAAHGQGGGCGYEQPGFSYHNSFLVADAAGAFVLETA
                     GPAWARERVTGVRSISNALTIAGFAEQHADRLRGRVAGSLSRRTWSQRHACTPDPSPA
                     TLMRALREHGTPDGLPRYTWHNGGLHAPCVHAGGLLAGSQTAASWVAELRPGSTQHWV
                     TATAAPCLGLFKPVAVATPVAPADMGPVPGARPSAALWWQHERLQRAVLRDPAAWMPE
                     IAAERDELEQRWLADPPDSAAAFVEHRRRLNAWLARVSATPSSDRRPWWVRRYTAARD
                     RVSGLTPKS"
     gene            complement(5631..6107)
                     /locus_tag="VGB85_02270"
     CDS             complement(5631..6107)
                     /locus_tag="VGB85_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132867.1"
                     /translation="MPAPVIDPQSTVRAARDRYLAVNGFSIAGYTEPTFTLTFLGRQI
                     TLPNPPARQAAIARHDIHHVLVGYDTDLAGEAEIGVWELRGGCPTAFLLFINGMALLA
                     GIFIAPRRVWRAWQRARGSHTLYVDGLDYEAALDMRVVDLRRRLGLPDEGIVARAG"
     gene            complement(6114..6752)
                     /locus_tag="VGB85_02275"
     CDS             complement(6114..6752)
                     /locus_tag="VGB85_02275"
                     /inference="COORDINATES: protein motif:HMM:NF019559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HEY0132868.1"
                     /translation="MRTPGSLLLRLALGAVVAYALAWLCGWPVLAMFSKPVPVLCLLA
                     FLLPPRTRDATLIGLGLVCSAIGDVLLEAGTILFLPGLCAFLLAHVLYVAAFLGRTTK
                     LHLRRLVPVAVLAWAVFSWLSPHLGVMRVPVLIYVVVISAMVWRAAAQVGEDRTGALQ
                     PWLATIGAVLFALSDVMVAYTRFIDPAAALKLPLMLLYWLGQTAITGSSRRA"
     gene            complement(6923..7117)
                     /locus_tag="VGB85_02280"
     CDS             complement(6923..7117)
                     /locus_tag="VGB85_02280"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEY0132869.1"
                     /translation="MNQDQANGTWDVVKGKAKKIWGELTDDDLLKAEGSADKLYGVIQ
                     ERFGDTKEAIQKKLSELHLP"
     gene            complement(7416..7847)
                     /locus_tag="VGB85_02285"
     CDS             complement(7416..7847)
                     /locus_tag="VGB85_02285"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY0132870.1"
                     /translation="MRFHETLHGVYFDDLDAFHILHNARYLLLFEHAIGSFWRTLGWA
                     GVLDFNSHPDQYHLVRANHLEYKRPVVGTGQVRVRVWVDRLGRSSLSFGLRVMPLDED
                     VDFASGERVVVRVDPETKRPVPWTTSFREQIAPYCHEIAAE"
     gene            7972..8811
                     /locus_tag="VGB85_02290"
     CDS             7972..8811
                     /locus_tag="VGB85_02290"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEY0132871.1"
                     /translation="MDAATEHLRARFAELAAAPDAALDLGELAAIIGAEEDPPADVEA
                     VMTSLDELAAVVAPRLRAVTRPREKLDLLLAYLVRELGFRGDETEYDDPRNSCLHAVL
                     ARRVGLPITLTVLLMEVGQRAGLPVVGVGMPAHFLALAPDLPGVYIDMFHGGRLLSEH
                     ECKNLLRQKTGGGVEFDREMLRPVSTRQILLRMLQNLKHTHTRRGKLDRALAASDRIL
                     LLAPAQAEEHRDRGLLQLQRRAFRAAADDLNHYLRLAPMADDRHVIAARANEARTRAK
                     QAN"
     gene            complement(8818..9105)
                     /locus_tag="VGB85_02295"
     CDS             complement(8818..9105)
                     /locus_tag="VGB85_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132872.1"
                     /translation="MRLIASIACLTALLLAPALASAAPARSDDDADSAARRTTPAGST
                     KEFIYGDEILEGELQSSDHERVDFRPPGKHPSLIKVRAHFIPQLLQMANDV"
     gene            9228..10169
                     /locus_tag="VGB85_02300"
     CDS             9228..10169
                     /locus_tag="VGB85_02300"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0132873.1"
                     /translation="MRGRRIVLLASLALGCAQKPPGEHVQPIVAAERAFSERSLERGT
                     KAAFLEFLAADSTVFAPGPRPGRAFYQSAPDVAGKLAWKPSYAEMSAGGDFGFTTGPY
                     TLTHADGRLHFGHYATVWHRQGRAWKVVVEGVTRHAPPLQVDENLTYPPAAKGRGAGK
                     VVVAAELRRLAAAERVLLLAWKTRGSAALLEYATDDVRYLPADALPMQGRGAVQAGLR
                     VLHDELSFEPGGGGVAPSGDLGYTVGAVTRRPGPSAPPVAGGYLRVWRRSARGVWLVA
                     LELHVLPPRAEGPAVPRISAPPDEPEYADDMPVAPAQ"
     gene            complement(10189..11529)
                     /locus_tag="VGB85_02305"
     CDS             complement(10189..11529)
                     /locus_tag="VGB85_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0132874.1"
                     /translation="MPIKLPPGPRFSLLQTALYARDPYGYLAAMQRKYGDPFTIPALN
                     GTLVITGTPAYIQQIFAADPDTFLSFGAKAIEPVVGPNSLLLLTGARHRRDRKLLTPP
                     LHGARMRAYGETIRQAALAAMATWREGQPFVMQTSTQWISLEVILRAVFGVQGDERIE
                     AFRAAIIELVAAAVPSLLFFTWLRRPWGGLGPYNRYVKAHAVIDGLVRGEIAARRATP
                     DVPREDIMSLLLSARDEAGEGMHDDELRDELMTLLFAGHETTGIALAWACYWLHRDPA
                     VLERLRAEIDALGDAPEPDAIAKLPYLDAVCNETLRLHPIAPDVPRMLGRTFELAGYK
                     LGPPYGVDVATTLVHRREELYPEPDTFRPERFLERRFTPFEFTPFGGGHRRCIGAAFA
                     TYELKIVLATFLRHAHLELAEPGEVLPARRNVVLGPATGVRMRLRERRAQRSAA"
     gene            complement(11592..12896)
                     /locus_tag="VGB85_02310"
     CDS             complement(11592..12896)
                     /locus_tag="VGB85_02310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HEY0132875.1"
                     /translation="MFGRKTTWTNWSGTVRCTPQQIVRPGSIDALIGVVRETAAREGR
                     LRIVGSGHSFSPVAATDGTMVSLQNLRGISELDREGRSATILAGTPINVLGDMLAEHG
                     LALTNQGDIDAQAIAGAVSTGTHGTGAAFGCLSTQVSGLTLVTAGGELLHCSATREPE
                     IFKAAQVSLGSLGALARVRLQLEPAYVLRDVRKNMPLETCLAEFEDSCRRHRHYEFWW
                     FPYSDVAATKALDIVPHARPRSRLKRVLVDKVLETGIFWAISEGVRRVPSSARPVARF
                     SAKFMSEGEYADASHRVFPSPRDVRFNEMEYAVPVERGVDCLREIRAFIEGQNIRVHF
                     PIEYRVVAGDDIWLSPFYQRDSAVISVHMYAGMPFDAYFAGCEAIFRNHRGRPHWGKM
                     HTLTARELQDMYPQWSRFHAVRQQLDPRGLFMNDHLTRIFGA"
     gene            complement(12920..>13281)
                     /locus_tag="VGB85_02315"
     CDS             complement(12920..>13281)
                     /locus_tag="VGB85_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132876.1"
                     /translation="YQLFKLRPAAGFALPVVRRPVPGIYTCFGGGWIASGAPGPDRLP
                     RPYLPVGARLLPTEGAGEVQTPIAFPGSLELGDPVLFRHAKAGELCEHVHELLLLGPD
                     GARERVPTYRGDGFAFV"
BASE COUNT         1979 a         4557 c         4944 g         1801 t
ORIGIN      
        1 cgagaagcac acctggcggg cctggaccac cgagcactgc gtcaacttcg tcgagatccc
       61 gaccgagccc tacgccttcg cccagaagac cacccgctcg gtgtacaacg acgacctgct
      121 gtgcgcgaag ttctgggagg agctgctggc gggcggcggc atccaggcgg tcgaggcgga
      181 ggttctcaat gggctcgacc acctgttcaa caacggcgtc tcgtcgccgg cgatggtcgg
      241 catcccgcag ccgatcaaga cggagatgta cgtctggccg ggcggctggt actacgtgcg
      301 cgccggcgcc gatatctcga accagcagat caccgactgg tcgcgcgagc ccctgcccgg
      361 cgaggagaac ctgatgctgg tcggcgaagc ctactggccg cagcggccag gttggtcgat
      421 cggcgcctat aactccgccg acaagctgct ggaggccaaa ttcggcatcg tacgggtgct
      481 cgaccggcag gtgcgcgcgg gctcgccgcg caagaaccgc gcgcagggcg ggcactagcc
      541 ggctccgcag cgggccccgg ggcggcctcg cgcggcgaaa gtgaggggcc cgcgaccctg
      601 tgctatacac cggcccggtc atgcgggcac tcggtctcga cgtgggtagc aagacgatcg
      661 gcgtcgcggt cggcgacccg agcggcacga tcgccagcgc gcggaccgtg ctcgcgcgcc
      721 gcggtcacgt cgccgacgcc gccgcgatcg ccgagctcgc gcgcaccgag gccgccggcg
      781 cggtcgtcgt cggcctgccg ctcgagctcg acggcaccgt cggacaccgc gcgcgcctgg
      841 tgcagcgctt cgtcgacgcg ctcgcagctg tcctcaaaga catgcccaac cccgtgaagc
      901 tccacacctg ggacgagcgc ttctccaccg ccgccgcgga gcgcacgatg atcgaggcgg
      961 acgcctcgcg cgcgcgtcgc aagcagagca tcgacgccgt cgcggcccag ttcatcctgc
     1021 agggctggct cgacgcccag cgccgccggg agggcctcgc atgagcacgt cactgccacc
     1081 gccgccggct cctccgtctc ctccatctcc tccatctcct ccatcatcct ccgacaaggg
     1141 atcgatcacg cccgtgaaga agccgcgtcg tcgctggttg cgccgctctc gctggtcgca
     1201 gtatgtcgcg ctcgcggtgg tcgcgacgac gctggtcgtc gcggcgctgg tcggctatcg
     1261 cgcctacgat cagctcgtgc agtacccgct gcgtgcgggt cgcggcgacg cgacgccggt
     1321 cgtgctgacg gtcccgcgtg gggcctcgtt cccgcaggtg ctcggccagt tgaccgaggc
     1381 cggtgtcgtg cctgcggacg aggcgctgta cttcaagctg ttcgtcctgc accgcggggc
     1441 ggcgagcaag gtcaccgcgg gggagcacac cttccgcggc gacatgaccc ccgaggagat
     1501 cctcgcggag ctggtacgcg cccagcgagc cgaggagatc cgcgtgacga tccccgaggg
     1561 tcgcaacatc ctcgaggccg cggacatcct cgccgccgcg ggcatcggca cggtcgagca
     1621 gatcctcgcg gcgatgcgcg accccgcgct gctcgaggag ctcggcatca ccggcccgag
     1681 cgtcgagggc tacctgttcc ccgacacgta caagttcccg gccaaggcga cgccgcagca
     1741 ggtgctgcgc cgcatggtcg agcagcaccg caaggtctac gccgggctgg cccgcggcca
     1801 ccgcgaggcg atcgaggagc tgcacaagac gctcggctgg ggtgaccgcg agatcgtcat
     1861 catggcgtcc ctggtcgaga aggagaccgc ggcgaagcac gagcggccgc tgatcgccgg
     1921 cgtgttcctc aaccgcctgc gcttccagtc gttccagccc aagcgcctcg agaccgaccc
     1981 gacgatcatc tacggctgca cggccgtggt cgagaagtcg gaggcgtgca aacagttcac
     2041 cgaccgcatc cgcaggatcc atctgcgtga cacggagaac ccgtacaaca cgtacacaca
     2101 cgagggcctg ccgccggggc caatcacaaa tcccggcaag gcggcgctcg aggccgtgct
     2161 cgcgccgaag aagtcgcgct tcctgtactt cgtgtcgcgc aacgaccgga cccaccagtt
     2221 ttccaaatcg atcgccgaac acgaggccgc ggtcgagaag tacatgcgcc agggcgcggt
     2281 cggcgatggc tcggccgccg gcgccgcggc ggccgactga gcctatcgcg tgatcatggg
     2341 cccgacggcg gcccgtcgct caccgtgaac agcacccagc ggccgtggtt gcgcgtcatc
     2401 aggagctgca ccggccgggc ctcctcggtc gcggagatcg atcgtcgggc gccctgctgc
     2461 gactgcgcga cgatcgtcca gggcgcgttc tgcagccggc gcaggagctc cgggagtcgg
     2521 ccctcgtccc agagcaggct gtcgtcgatg ccgcggatga accgggcgag cgagcgcgcc
     2581 cgcgtcgcgg cgtccggcgc cgcggtgctc gccgtggcga ggcgagcgag ttcggccagc
     2641 acctcctccg gagatccctc gaggccgatc gggtacacgg tctcgctgcc cgggctggcc
     2701 atctcgcggc gcagggtcgc gacggccccg gtcacgaggc gaccgcgcac gtcgccgagg
     2761 tcgagcaaga cctcgggctc tccgatacgc gcgagcagcc cgcgctcgtt gttctggctg
     2821 aagtcccaga cctcgacgcg ggccggcctg tcgccgctgg cgaccgccac gacgatgcgg
     2881 gcccggccgc gcacgccgtc ggccagctcg gccaccgcct cggcgtgcac ccccgccggg
     2941 atcgccgcgg ccagggcagg cgcgtagcgc cagggctccg cgtccggcga ggtacttgcg
     3001 ggtgttgcgg agggctccgg ggccgcagcc gcgacgggcg cgacagccgg cggcgccggg
     3061 gtcgttgcat cggcagcgtt cgcggccggt ttggggtcgc aagccgccag cgcgaaggcg
     3121 gacgcgagca cagcccacgc gggccgcggc gaatttcgag cgccacgcac ttgtgatagc
     3181 gtcggctgta gagccgccgg ggtcaagcgt gatagcttgc aggtggcatg gtgttcgcgc
     3241 ggcgtagcct ggcggtggtg ctcgccacca gtgtggtctt cagctcctct ggacgggggg
     3301 tcgcggaggc cgcgatgttc gcgcctgcgg ccgctggtgc atcagctgcg tcaggctcgg
     3361 gcgccaagct ggcgatcttg ccgttgacga tcgaaggtgg gctcggcgag gccgaccagg
     3421 ccgagctgac caaggcgctc tcggcgggcc tgcaacgcgg caacttcagc atcgtcagcc
     3481 ccgagcaggt gctggcgacc gacgcccgcg ccggcagctg cgacaacacg gtctgctatc
     3541 agaacatcgc gcgacgcacc ggggcggcct acgtcgtgcg tgcggtcgtc agcgtcaagg
     3601 accgcgacta cacggtgaag gtcgagctcg tcggtgcgga cggcaagcgg ctcgcggcga
     3661 cccaggacgg ctgcgagatc tgcggcgtcg tcgatgcgac cgggctgatc gactccgccg
     3721 cggcgaccct tcgcctcaag ctcgacgcgc tgaccaaggg cccggccgcg ctcaagctgg
     3781 cgaccgaccc cgcgggtgcg atcgtcacga tcgacggcga ggtcgtcggc gaggcgccgc
     3841 tcgagcgacc gatcgtgccg ggcaagcacc tgatccgcgt cagcgaggag ggcttcatct
     3901 cgatcgagcg cgaggtcacc ttcgtcgagg gcgtcagcga ggacctcacc ttcaagctcg
     3961 agaagctgcc gagccgcctg ccggggcgcc gcttcggcta cgtctcgctc ggcttcggcc
     4021 tcgcggcgct cggtggcgcg atcggcttcg cgatcctcga cgacaagccc tacaagctcg
     4081 gcggcaactg cagcacgacc tacgggggcg accccgacgc gcccttcaac aacatcccgg
     4141 gccaggactc gatcaccggt cgcgacgagt gcgccaagct gtggcgcacc gagccgttta
     4201 gcatccccct ggcgatcgcc ggcggcgcgc tgctgaccct cggcatcgcg atcttgctca
     4261 actcgcgcaa gcgcacccag gccgagaagg gcccgaccgc ccggcgcggc atgccgcgct
     4321 tcggcgtcgg ccccggcagc gtcagcgtca gcggccgctt ctagtcgtca gcttttgggc
     4381 gtcaggccgg acacgcggtc gcgcgccgcg gtgtagcgac gcacccacca cgggcggcgg
     4441 tccgacgatg gcgtggccga cacgcgcgcg agccaggcgt tgaggcggcg gcgatgctcg
     4501 acgaaggcgg ccgcgctgtc gggaggatcg gcgagccagc gctgctcgag ctcgtcgcgc
     4561 tcggccgcga tctccggcat ccacgcggcg gggtcgcgca gcacggcccg ctgcaggcgc
     4621 tcgtgttgcc accacagcgc cgccgacggc ctggccccgg ggacaggtcc catgtcggcg
     4681 ggcgcgacgg gggtggcgac ggcgaccggc ttgaacaggc cgaggcacgg cgccgcggtc
     4741 gcggtgaccc agtgctgcgt gctcccgggt cgcagctcgg cgacccacga ggcggcggtc
     4801 tgcgagccgg cgagcaggcc cccggcgtgc acgcagggcg cgtgcaggcc gccgttgtgc
     4861 caggtgtagc gcggcaggcc gtcgggggtg ccgtgctctc gcagcgcgcg catgagggtc
     4921 gcgggggacg ggtcgggggt gcaggcatgg cgctgcgacc atgtccttcg ggacaggctc
     4981 ccggcgacgc gcccgcgcag gcggtcggcg tgctgctcgg cgaagccggc gatcgtcagc
     5041 gcgttggaga tcgatcgcac cccggtgacc cgctcgcgcg cccacgcggg gccggcggtc
     5101 tcgagcacga aggcgccggc cgcgtcggcg accaaaaagc tgttgtgata cgagaagccc
     5161 ggctgctcgt agccgcagcc gccgccctga ccgtgcgccg ccagcagcgc gacgatcacc
     5221 tcgcaggccg cctcggcgct gtcggagcgc tcgagcgcca ggcgcacgag gtccatgccg
     5281 gtcaggccga cgcgcgcgag gggacttcgg gtgaacaccg cctcgttgcc gatgacgacg
     5341 ccgtgctcgt tggtccccat ctcggcgccc caggtccaaa acgggcggct cagcaggacc
     5401 gccgccgtct ccgccacctg cggcaccgag atccacgtgc acgcgagcgc ggcgccgggc
     5461 gcgtgctggc ggcgcgggtg ccactcgagc agctgcgcct cgccgaggtc gcggtcggag
     5521 ctcttggcga acagcacctg ccccggccgc acgatgacgg tcgtgtcaca catccgcggg
     5581 cagcctagca cgcgcgcggc gagccgcctc atgtcccgaa ggacaggtcg tcagccggcg
     5641 cgcgcgacga tgccttcgtc gggcaggccg aggcggcggc gcaggtcgac gacgcgcatg
     5701 tcgagcgcgg cctcgtagtc gaggccgtcg acatagagcg tgtgagagcc gcgcgcgcgc
     5761 tgccacgcgc gccagacccg acgcggcgcg atgaagatgc cggcgagcag cgccatgccg
     5821 ttgatgaaca gcaagaacgc ggtcgggcac ccgccgcgca gctcccacac gccgatctcg
     5881 gcctcgccgg cgaggtcggt gtcgtagccg acgagcacgt ggtgaatgtc gtggcgggcg
     5941 atcgcggcct ggcgcgcggg cgggttgggc agcgtgatct ggcgcccgag gaacgtcagc
     6001 gtaaaggtcg gctcggtgta gccggcgatc gagaagccgt tgaccgcgag gtacctgtcg
     6061 cgcgcggccc ggacggtgga ctggggatcg atgacggggg ccggcatggg atctcaggcg
     6121 cggcggctcg agccggtgat cgccgtctgg ccgagccagt acagcagcat cagcggcagc
     6181 ttgagcgcgg ccgcggggtc gatgaagcgg gtgtaggcga ccatgacgtc gctgagcgcg
     6241 aacagcacgg cgccgatcgt cgccagccat ggttgcagcg ccccggtccg gtcctcaccg
     6301 acctgcgccg cggcgcgcca caccatcgcg ctgatgacga cgacgtagat cagtaccggc
     6361 acgcgcatga cgccgaggtg cggcgacagc cacgagaaca ccgcccacgc gagcacggcg
     6421 accgggacca ggcggcgcag gtgcagcttc gtcgtgcggc cgaggaaggc ggcgacgtag
     6481 agcacgtgcg cgagcaggaa cgcgcacagg ccgggcaaga acaggatggt gccggcctcg
     6541 agcagcacgt cgccgatcgc cgagcacacc aggccgaggc cgatcagcgt cgcgtcgcgg
     6601 gtgcgcggcg ggagcaagaa ggccagcagg cacaggacgg gcaccggctt gctgaacatc
     6661 gccaggaccg gccatccgca cagccacgcg agggcgtagg ccacgacggc gccgagggcc
     6721 agacggagga ggagggaccc cggggtgcgc atcgcccgat actatcagcg gcgccgggtc
     6781 cgtgtccgtg cgacgcgaag ccgcagcggg gagcctgctc gcgaccgacc ccgggcgccg
     6841 cgcgggcgtc cagggtcggg aggtgtgaac ccggaggatc cgggagcaca tgagcacatg
     6901 atcactggat caggtcgact gatcagggca ggtgcagctc ggagagcttc ttctggatcg
     6961 cttccttggt gtcaccgaag cgctcctgga tgacgccgta cagcttgtcg gcggagccct
     7021 cggccttgag aagatcgtcg tcggtcagct cgccccagat cttcttcgcc ttgcccttga
     7081 ccacgtccca cgttccattg gcttgatctt gattcatcac agtctccgtt tcgctggcca
     7141 gaaggtaggt gcagtcgccc acaagtcaag cgagcggccg cggcggcgat ttttcgaggt
     7201 gtcgccattc ccgagataac gccactggca aactgtcatt gctcgtcgcg gatcctgcgc
     7261 gcggttcggc ctgcgctggg agagcggcgt acgcgggcca caaacggcga acccgggcgc
     7321 tggcttgcgc gccctgcccg gcgcgctccg gatccgacgc gcgccgaatc ccatcgataa
     7381 ttcttcacat gtcccgaaga acatatgaag cggcgtcact ccgcggcgat ctcgtggcaa
     7441 taaggcgcga tctgctcgcg gaagctcgtc gtccacggga ccgggcgctt ggtctcgggg
     7501 tcgacgcgga cgacgacccg ctcgccggag gcgaagtcga cgtcctcgtc gagcggcatg
     7561 acgcgcaggc cgaagctcag gctgctgcgg ccgaggcggt cgacccacac gcgcacgcgg
     7621 acctggccgg tgccgacgac ggggcgcttg tactcgaggt ggttggcgcg cacgaggtgg
     7681 tactggtcgg ggtgcgagtt gaagtcgagg accccggccc acccgagggt gcgccagaac
     7741 gacccgatcg cgtgctcgaa cagcagcagg tagcgcgcgt tgtgcaggat gtgaaacgcg
     7801 tcgaggtcgt cgaagtagac gccgtgcagg gtctcgtgga agcgcatcgc agccctgctg
     7861 taccgtgaat gccgcgcggc gaccaccgcg ccggcacccg cgccgtcgcg gccatgctca
     7921 cacggccagg aaaccccgcc tgcgtggttt ctgagctatg ttcgccggct gatggacgcc
     7981 gccaccgagc acctgcgggc ccgctttgcg gagctcgccg ccgcgcccga cgcggccctc
     8041 gacctcggcg agctcgccgc gatcatcggc gcggaggagg acccacccgc cgatgtcgag
     8101 gcggtgatga cgagcctcga cgagctggcc gccgtcgtcg cgccgcgcct gcgcgcggtg
     8161 acacggccgc gcgagaagct cgacctgctg ctcgcgtacc tggtgcgcga gctcggcttt
     8221 cgcggcgacg agaccgagta cgacgacccg cgcaacagct gcctgcacgc cgtgctcgcg
     8281 cggcgtgtcg gtttaccgat cacgctgacg gtgctgttga tggaggtcgg ccagcgcgcg
     8341 ggcctgccgg tggtcggggt cgggatgccg gcgcacttcc tcgcgctcgc gccggacctg
     8401 ccgggcgtgt acatcgacat gttccacggc ggccgcctgc tcagcgagca cgagtgcaag
     8461 aacctgctgc gccagaagac cggcggcggc gtcgagttcg accgcgagat gctccggccg
     8521 gtctcgacgc ggcagatctt gctgcgcatg ctgcagaacc tcaagcacac gcacacgcgc
     8581 cgcggcaagc tcgaccgggc gctcgcggcc agcgaccgca tcctgctgct cgcgccggcc
     8641 caggccgagg agcaccgcga ccgcgggctg ttgcagctgc aacgccgggc gtttcgcgcg
     8701 gctgccgacg acctcaatca ctaccttcgc ctcgcgccga tggccgacga ccgccacgtg
     8761 atcgcagccc gggccaacga ggcccgcacg cgcgccaaac aggcgaactg agcgcgatca
     8821 gacgtcgttg gccatctgca gcagctgcgg gatgaagtgg gcgcggacct tgatcaggct
     8881 cgggtgcttg ccgggcgggc ggaagtcgac gcgctcgtgg tcggagctct gcagctcacc
     8941 ctcgaggatc tcgtcgccgt agatgaactc cttggtcgag ccggcgggcg tggtgcgacg
     9001 cgcggccgag tcggcgtcgt cgtcgctgcg ggccggggcc gcggaggcga gggccggcgc
     9061 gaggaggagg gcagtaagac aggcaatgct ggcgatcagg cgcatggtgg tctccgggtc
     9121 acgagggtga cggggtcgtg ctcgctgcaa cgtcgcgttc acgggccggg tctggcgccg
     9181 cggcgatttt ttgtcgggcc gccccgcgct cgcggtatat cggcgagatg cgagggcgca
     9241 ggatcgtgct gctggcgagt ctcgctctcg gctgcgcaca gaagccgccc ggggagcacg
     9301 tgcagccgat cgtcgccgcc gagcgagcgt tcagcgagcg ctcgctcgaa cgcgggacca
     9361 aggcggcgtt cctcgagttc ctcgccgccg actcgacggt gttcgccccc gggccccggc
     9421 ccggtcgcgc gttctatcag agcgcccccg atgtcgccgg caagctggcc tggaagccca
     9481 gctacgcgga gatgtcggcc ggtggcgact tcggcttcac caccggtccc tatacgctga
     9541 cccacgccga cggacgcctg cacttcggcc actacgcgac ggtgtggcac cggcagggcc
     9601 gcgcgtggaa ggtcgtcgtc gagggcgtca cccgccacgc accgccgctg caagtcgacg
     9661 agaacctgac gtacccgccg gccgcgaagg gccgcggcgc cggcaaggtg gtcgtcgccg
     9721 cggagctgcg ccggctcgcg gccgcggagc gcgtgctgct gctcgcctgg aagacgcgcg
     9781 gcagcgcggc gctgctcgag tacgcgaccg acgatgtccg ctacctcccg gccgacgcgc
     9841 tgccgatgca gggccgcggc gcggtgcagg ccggcctgcg cgtgctgcat gacgagctgt
     9901 cctttgagcc aggcggtggc ggggtcgcgc cgtccggtga cctcggctac acggtcggcg
     9961 cggtgactcg ccggcccggt ccgagcgcgc cgccggtcgc gggcgggtat ctgcgggtgt
    10021 ggcgacgcag cgcgcgcggg gtgtggctgg tggcgctcga gctgcatgta cttccgccgc
    10081 gcgccgaggg cccggcggtg ccgaggatct ccgcgccgcc ggacgagccc gagtacgccg
    10141 acgatatgcc tgtcgcgccc gcgcagtgac ctggcccgaa gggcaggttc acgccgcgga
    10201 gcgctgggcc ctgcgctcgc gcaggcgcat gcggacgccg gtggccgggc cgagcacgac
    10261 gttgcggcgc gcgggcagga cctcgcccgg ctcggcgagc tcgaggtggg cgtggcgcag
    10321 gaaggtcgcg agcacgatct tcagctcgta ggtcgcgaag gcggcgccga tgcagcgccg
    10381 gtggccgccg ccgaagggcg taaactcgaa gggagtaaag cggcgctcga ggaagcgttc
    10441 ggggcggaag gtgtcgggct cggggtacag ctcctcgcgg cgatgcacga gggtggtcgc
    10501 gacgtcgacg ccgtacggcg ggccgagctt gtagccggcg agctcgaagg tgcggccgag
    10561 catgcgcggc acgtccggcg cgatcgggtg caggcgcagg gtctcgttgc agacggcgtc
    10621 gaggtagggg agctttgcga tcgcgtcggg ctcgggggcg tcgccgaggg cgtcgatctc
    10681 ggcgcgcagg cgctccagca ccgcggggtc gcggtgcagc cagtaacagg cccacgcgag
    10741 cgcgatgccg gtggtctcgt ggccggcgaa cagcagggtc atcagctcgt cgcgcagctc
    10801 gtcgtcatgc ataccctcgc cggcctcgtc gcgggccgag agcagcagcg acatgatgtc
    10861 ctcgcgcggc acgtcgggcg tcgcacggcg ggccgcgatc tcgccgcgca cgaggccgtc
    10921 gatcaccgcg tgggccttga cataacggtt gtagggaccg aggccgcccc acggccggcg
    10981 cagccaggtg aagaacagca ggctcggcac ggcggcggcg accagctcga tgatcgcggc
    11041 gcgaaacgcc tcgatgcgct cgtcgccctg cacgccgaac acggcgcgaa ggatcacctc
    11101 gagcgagatc cactgggtcg aggtctgcat cacgaagggc tggccctcgc gccaggtcgc
    11161 catcgcggcg agcgcggcct ggcggatcgt ctcgccgtag gcgcgcatgc gcgcgccgtg
    11221 cagcggcggc gtcagcagct tgcggtcgcg gcggtggcgg gcgccggtca gcagcagcag
    11281 ggagttgggg ccgacgaccg gctcgatcgc cttggccccg aacgacaaaa aggtgtcggg
    11341 gtcggccgcg aagatctgct ggatgtacgc gggcgtgccg gtgatgacga gcgtgccgtt
    11401 gagcgccggg atcgtgaagg ggtcgccgta cttgcgctgc atcgcggcga ggtaaccgta
    11461 ggggtcgcgc gcgtagaggg cggtctgcag cagactgaag cgcgggcccg gcgggagctt
    11521 gatcggcatg cgccgcaaga tgccacaccg cggcggccag ggcgccggac gcgggacaag
    11581 gtgtcgcagg atcaggcgcc gaagatgcgc gtcaggtggt cgttcatgaa caggccgcgc
    11641 gggtcgagct gctggcggac cgcgtggaag cgcgaccact gcggatacat gtcctggagc
    11701 tcgcgggcgg tcagggtgtg catcttcccc cagtgcgggc ggccgcggtg gttgcggaag
    11761 atcgcctcgc agccggcgaa gtaggcgtcg aacggcatgc cggcgtacat gtgcacggag
    11821 atgacggccg agtcgcgctg gtagaacggg ctgagccaga tgtcgtcgcc ggcgacgacg
    11881 cggtactcga tcgggaagtg gacgcggatg ttctggccct cgataaaggc acggatctcg
    11941 cgcaggcagt cgacgccgcg ctcgacgggc acggcgtact ccatctcgtt gaagcggacg
    12001 tcgcgggggc tcgggaacac gcggtggctg gcgtccgcgt actcgccctc ggacatgaac
    12061 ttcgcggaga agcgggcgac cgggcgcgcc gaggagggca cgcgccgcac gccctcggag
    12121 atcgcccaga agatgccggt ctcgagcacc ttgtcgacga gcacgcgctt gaggcgagag
    12181 cgtggccggg cgtgggggac gatgtcgagg gccttggtcg cggcgacgtc gctgtacggg
    12241 aaccaccaga actcgtagtg gcggtggcgc cggcagctgt cctcgaactc ggcgagacat
    12301 gtctcgaggg gcatgttctt gcggacatcc cggagcacgt aggcaggctc gagctgcagg
    12361 cgaacgcggg cgagcgcgcc gaggctgccg agcgagacct gcgcggcctt gaagatctcc
    12421 ggctcgcggg tggcggagca gtgcagcagc tcgccgccag cggtgacgag ggtcagcccg
    12481 gagacctgcg tcgacaggca gccgaacgcg gcgccggtgc cgtgtgtgcc ggtggacacg
    12541 gcgccggcga tcgcctgggc gtcgatgtcg ccctgattcg tcagcgcgag gccgtgctcg
    12601 gcgagcatgt cgccgagcac gttgatcggc gtgccggcca ggatggtcgc gctgcggccc
    12661 tcacggtcga gctcggagat ccctcgcagg ttctgcagcg agaccatggt gccgtcagtc
    12721 gcggcgaccg gcgagaacga gtggccgctg ccgacgatgc gcaggcgacc ctcgcgcgcg
    12781 gcggtctcgc ggacgacacc gatcagcgcg tcgatgctgc cgggacgaac tatttgctgc
    12841 ggcgtgcagc gcacggtgcc ggaccagttg gtccatgtgg tcttgcgtcc gaacacggcg
    12901 cgagatggta gcaggcgagt cagacgaagg cgaagccgtc gccacgatag gtgggcactc
    12961 gctcgcgggc gccgtccggg ccgagcagca gcagctcgtg gacgtgctca cacagctcgc
    13021 cggccttggc gtggcgaaac aggaccgggt ccccgagctc gagggagccc gggaaggcga
    13081 tcggtgtctg gacctcgcct gccccctcgg tgggcagcaa gcgggcgccc accggcaagt
    13141 acgggcgcgg caggcggtcg ggaccgggag cccccgaggc gatccagccg ccgccgaagc
    13201 aggtgtagat cccagggacc gggcggcgca cgaccggcaa ggcgaagcca gctgccggcc
    13261 gcaatttaaa taattggtag c
//
LOCUS       DASWNJ010000126         1806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_2506, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000126 DASWNJ010000000
VERSION     DASWNJ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2506"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VGB85_02320"
     CDS             <1..709
                     /locus_tag="VGB85_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0132877.1"
                     /translation="RTAEYLQGHDFVVEVAADGELGLQRALSGEHDVVLLDIMLPRRD
                     GLAVCKELRQRSAVPVLMLSARSEEIDRVLGLELGADDYLAKPFSPRELVARIRAVLR
                     RGAPTTGSPAPPGAPAAPNLRRCGPLTIDRDRHRASVEGTPCELTAYQFDLLWILAGA
                     PEQALSREQIHQRLRTLRGEPPADFDPAVDRSIDVHLSKVRAALAAANPSGAGLIRTL
                     RGVGYMLAPPDTKGPAS"
     gene            706..>1806
                     /locus_tag="VGB85_02325"
     CDS             706..>1806
                     /locus_tag="VGB85_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEY0132878.1"
                     /translation="MSQTRGGLFLRIYVTFILTVIAFAAIVGLVVLSLAGQYDAAWVE
                     SVDTAVAAREPALSRLVATIDDPAARAALEAEVAALARELDLRAAIHDRDGHLLAGDP
                     ATRSPRALKPRQLARLQRGQPVVQRGGERGERGPALGFGIPGPDGSVIAVLALEVGDR
                     GAERSRLLVLALLGLLAILAVGAWPLARSLTRRLAVLEEGAGRIARGELGHRIADPRA
                     SDELGRLGLAFNDMAAQLEAQVKGQRALLTNVSHELRTPVARMRVLAELLAERLAGMP
                     DAEHPATVRVRRGIAELGDDLIELETLIADLLTSGRLDLGGAATLQRSDTELAPLLTR
                     VAARVDARVRCDEALHAELDPVLIERLLANLLH"
BASE COUNT          217 a          783 c          586 g          220 t
ORIGIN      
        1 gcgcacggcc gagtacctgc aaggccacga cttcgtcgtc gaggtcgccg ccgacggcga
       61 gctcgggctc cagcgagcgc tgtccggcga gcacgacgtc gtcctgctcg acatcatgtt
      121 gccgcgccgc gacggcctcg ccgtctgcaa ggagctgcgc cagagatccg ccgtgcccgt
      181 cctgatgctc tcggcccgca gcgaggagat cgaccgcgtg ctcgggctcg agctcggcgc
      241 cgacgactac ctcgccaagc cgtttagccc gcgcgagctg gtcgcccgca tccgggccgt
      301 gctgcgccgc ggcgcgccga cgacgggctc accggcgccg cccggcgccc ccgcggcccc
      361 gaacctgcgc cgctgcggcc cgctgacgat cgaccgcgac cgccaccggg ccagcgtcga
      421 gggcacgccc tgcgagctga cggcctacca gttcgacctg ctgtggatcc tcgccggcgc
      481 ccccgagcag gcgctgtccc gcgagcagat ccaccagcgc ctgcgcaccc tgcgaggcga
      541 accacccgcc gacttcgacc ccgccgtcga ccgctcgatc gacgtgcacc tcagcaaggt
      601 ccgggccgcc ctcgccgccg ccaacccgag cggcgccggc ctgatccgca ccctgcgagg
      661 cgtcggctac atgctcgcgc cgcccgatac gaagggcccg gcgtcgtgag tcagacccgc
      721 ggcggcctgt tcctccgcat ctacgtcacc ttcatcctca cggtgatcgc cttcgccgcg
      781 atcgtcggcc tcgtggtgct cagcctcgcc ggccagtacg acgccgcgtg ggtcgagtcg
      841 gtcgacaccg ccgtcgccgc gcgtgagccg gcgctcagcc gcctcgtcgc cacgatcgac
      901 gacccggccg ctcgcgccgc gctcgaggcc gaggtcgccg ccctcgcccg cgaactcgac
      961 ctgcgcgccg cgatccacga ccgcgacgga cacctgctcg ccggagaccc cgcgacccgc
     1021 agcccccgcg ccctcaaacc ccgccagctc gcccgcctcc agcgcggcca gcccgtcgtg
     1081 caacgcggcg gcgagcgagg cgagcgcggc ccggcgctcg gctttggcat ccccggcccg
     1141 gacggctccg tgatcgccgt gctcgcgctc gaggtcggag accgcggcgc cgaacgcagc
     1201 cgcctgctcg tgctcgccct gctcggcctg ctggcgatcc tcgccgtcgg cgcgtggccg
     1261 ctcgcgcgct cgctgacccg ccgcctcgcc gtgctcgagg agggcgccgg ccggatcgcc
     1321 cgcggcgagc tcggccaccg gatcgccgac ccgcgcgcca gcgacgagct cggccgcctc
     1381 ggcctcgcct tcaacgacat ggccgcgcag ctcgaggccc aggtcaaggg ccagcgcgcc
     1441 ctgctgacca acgtctcgca cgagctgcgc acccccgtcg cccgcatgcg cgtgctcgcc
     1501 gagctgctcg ccgagcgcct cgccggcatg cccgacgccg agcaccccgc gaccgtccgc
     1561 gtccgtcgcg gcatcgccga gctcggcgac gacctcatcg agctcgagac cctgatcgcc
     1621 gacctgctga cctcgggccg cctcgacctc ggcggcgccg cgaccctgca gcgcagcgac
     1681 accgagctcg ccccgctgct gacccgcgtc gccgcccgcg tcgacgcccg cgtccgctgc
     1741 gacgaggcgc tgcacgccga gctcgacccg gtcctgatcg agcgcctgct cgccaacctc
     1801 ttacac
//
LOCUS       DASWNJ010000127         8340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_389716, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000127 DASWNJ010000000
VERSION     DASWNJ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 8340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8340
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389716"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="VGB85_02330"
     CDS             <1..1117
                     /locus_tag="VGB85_02330"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0132879.1"
                     /translation="FGQAILQAPTQAFYIADTHAVEAVATEVSDLPRTVRAVAVLPLY
                     SRRHGSWQGIIVAHWPGVHPPAADERLLHTLLIHATAEAIASEQARARNETLLREARE
                     ALQDSQRQSNLLSALFEHLPVGVSLMRGDTGAVEFVNPAGLAALGQELPSEGIEVTGA
                     QIYVAGESEPLTPERFPVNIALRTGQRVRTELEMVSPSGTRRIFDATAVPIKAGEDGV
                     VRALMLFHDLTYVRQAERERFAAQEQLLRMQSAALLERSTPLIPVREDVLVMPLIGSI
                     DPERGRQILETLTHLGGRTHVRTVILDLTGVRSVDTASASALINAAMAVRLRGVRPLL
                     TGIQPAVAATLVSLAVDFGSLEVFASLQDAVAQATRR"
     gene            1179..2240
                     /locus_tag="VGB85_02335"
     CDS             1179..2240
                     /locus_tag="VGB85_02335"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEY0132880.1"
                     /translation="MSMFLRRLGIDTPIIQAPMAGVSTPAMAAAVAEAGALGSIAIGA
                     VDVAAARNMIAAVRALTSRPFNVNVFCHRPPVADPDRERAWLSRLAPELARYGATPPA
                     RLRPIYTSFVEDDAMLAMLLAERPAVVSFHFGLPSSERIAALRAAGIVLFATATDLAE
                     ARAVAAAGVDVVVAQGIEAGGHRGVFDPAARDDGLGTLALARLLVRALDIPVIAAGGI
                     MDGAGIAACLRLGVAAVQLGTAFIACPESSADAGYLAALASQAAHHTVMTTAISGRPA
                     RCLPNLLTALGERVTADEVPDYPIAYDAGKALHAAARAHGEFGYGAQWAGQGAPLARS
                     LPAAELIAILQAELAEAQR"
     gene            complement(2241..3617)
                     /locus_tag="VGB85_02340"
     CDS             complement(2241..3617)
                     /locus_tag="VGB85_02340"
                     /inference="COORDINATES: protein motif:HMM:NF025391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4241 domain-containing protein"
                     /protein_id="HEY0132881.1"
                     /translation="MTRAAGHRYKPRSMSHESTLELLNDALAEACTSDGRRVRLDFRA
                     GEGEVVHFTAMLFDPPDELLDMRSWELPGSPVPAGTSEARLAGFLAGLRVASRSLDWD
                     FVYPDSFPPFGLLAASWLASEDFVGVLGSPDALGEALARLLTEGFAARHGLGPISRAD
                     AMRLHELDTFAVRQPMLAAHVDALAGAAAPVPAYLLALLERWADEIDARGEDGALTDG
                     GQPLRPHLATWAVSKLLQSLDEPDRARVARLRERGLIGRAPLDLEALFTAHEYAGVRL
                     SQVEVGVIDIPAGMVIVCDPYELEVRPILTGAPPGRYPVRLLLAELDPWGRRVAVARL
                     DLSTTRATRWRSTGHRFFVDAGLACFMSIGSRDALRASLAAFRTTHPDRNHYIDVLDP
                     VLRASAGPGRSSGSWGVLALGGAHQAAVFASGLGDGAYEVLAGVDDEGRTVALAVDFG
                     VLPEVERA"
     gene            3780..5180
                     /locus_tag="VGB85_02345"
     CDS             3780..5180
                     /locus_tag="VGB85_02345"
                     /inference="COORDINATES: protein motif:HMM:NF015657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132882.1"
                     /translation="MRSTLVPLLPLILFTTACGDNGGASETGSDTGTSTAPGTGTDPG
                     TDTDPTGGVAAGPTYWDDVAPIFFSSCVTCHQAGGVGPFVLDSYAEAKKWAPATVASV
                     EARMMPPWLVTDDGTCGTFQDSKALAQADIDTIRAWVDAGTPEGTPRSDLQAPAPETL
                     TDAIEFKTPKFSPAIQGGALAEFDEYRCFKVDTKLAADRFLTGYSVKPGTPAMIHHVL
                     AITVDPEQTGAGGKKNSEIIAALDAESPDRDGWPCFGAAGDGVNSGGIPVSWAPGQGV
                     TSLPEGIGFRIKKDDYLILQVHYNLVDPALEGIEDQTSLQIRLADTVEREGFFNLPDD
                     FLGSLFSGTPDQLEPGKAAVTYTFDHSLEYLPFLGASTADLYGILPHMHQRGRKFRVE
                     LIEDGGAPQCATDVKAWDFNWQLFYFFDKPLPLTTKSKLRVTCEFDTQSATEPVLPGW
                     GTQNEMCLAGLFIVPK"
     gene            5305..7407
                     /locus_tag="VGB85_02350"
     CDS             5305..7407
                     /locus_tag="VGB85_02350"
                     /inference="COORDINATES: protein motif:HMM:NF019256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HEY0132883.1"
                     /translation="MKASVCPQRTLTLTLLCAIGCSGGDAATSGTSGTADDPSTGANT
                     PTTGASVDPTTGTGEPPTTGPDDDTGDTGEPSEPPPEPSGNQLDQNKLFTCVGAPAMP
                     PADIRLLDRHEWTRNVGSWADSDLARNPLFARPDHRYSSYGDGEALDPSVLGLYLDVV
                     GGAGEAWARGKYEPGRVRTVIEDPEAQCFFKDAAPSTECVRYFVRRYLERGAMYRPAS
                     AEQVDALVEFSEATLDGEADIDARPATIKRIAAAAWMSSGALHRPELGEGAPDEHGRL
                     RLGDWELAQSIAYALSRGPAGAPSVNRSFNDGFSKGDIDGDLGGFFHAATDGSIQDPA
                     VVAELVREYVGGLDELRADLVLEPRDGRWWENRGEYWMAIGVRGFFREWLDYGELADQ
                     PPKVEVAATSAWSGATVENSYQNQISGFYGYESTLVQQLDDMIARIVADDKDVFAALL
                     TSRKFYTPATAGYQEGETSIWKSTAEANRVYNVQGVTAQTREARWIDLPEAERAGVLT
                     HPAWLGAHSLSFENDPNLVHRGKWIREELLCNDLPDLPLNVDAMLPEASKSQSARQRI
                     SEKIDADPYCSGCHSFMNPLGYPFEIYNHAGFLRIEDHGAPPNGTSTLVNMPSPELNG
                     AVTSAIDLSQRFAGSPYAKRCFMRQTFRYFAGREETMADACTMVAIEKAYDDSGGSFT
                     EMLIALFTSDSFQMRAPD"
     gene            7425..>8340
                     /locus_tag="VGB85_02355"
     CDS             7425..>8340
                     /locus_tag="VGB85_02355"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEY0132884.1"
                     /translation="MFTRRKLLTTSGLLAGSAMTLGTLGAFGLGGLGGLTLGSRRTRA
                     AEGDPRRIVIFLEGNGTRPVCMRDPLTRETLEGFAGKPIESNRDYGHDDPVLIAKAPL
                     AEARALGSLAGAQDQISLVERSALVLGLSATYLGGGHSSDFGALSADKALGGPAAATI
                     DTVLAGIPEVRGTTPFDVVRVGVAAASTPLNYSSCAFAKGKPAPILISPADAYASLFG
                     SVASGQAGEDFQARKQLLEFAIEDLGRELKGFAGSSRAQAKLETYEASLMAMLARNEQ
                     ILGMKGKLLAHKPAGPGELDPDLYASIDP"
BASE COUNT         1255 a         3155 c         2829 g         1101 t
ORIGIN      
        1 gttcggacag gcgatattac aggcccctac gcaggcgttc tacatcgccg acacgcacgc
       61 ggtcgaggcc gttgcgaccg aggtctccga cctgccgcgc acggtccgcg ccgtcgcggt
      121 gctgccgctg tacagccggc gtcacggctc ctggcagggg atcatcgtcg cgcactggcc
      181 gggcgtccat ccacctgccg cggacgagcg gctgctgcac acgctgctga tccacgcgac
      241 cgccgaggcg atcgcgagcg agcaggcgcg cgcgcgcaac gagaccctgc tgcgcgaggc
      301 ccgtgaggcc ctgcaagaca gccagcgcca gagcaatttg ctgagcgcgc tgttcgagca
      361 cctgccggtc ggtgtgtccc tgatgcgcgg cgacaccggg gccgtggagt tcgtcaaccc
      421 ggccggcctc gccgcgctcg gccaggaact gccgagcgag gggatcgagg tgaccggggc
      481 gcagatctac gtcgccgggg agagcgagcc gctgacgccg gagcgcttcc cggtgaacat
      541 cgccctgcgc accgggcagc gcgtgcgcac cgagctcgag atggtgtcgc ccagcggcac
      601 gcgccggatc ttcgacgcca ccgccgtgcc gatcaaggcc ggcgaggacg gggtggtgcg
      661 ggcgctgatg ctgttccacg acctcaccta cgtgcgccag gccgagcgcg agcgcttcgc
      721 ggcccaggag cagctgcttc gcatgcagtc ggccgcgctg ctcgagcgat cgaccccgct
      781 gatccccgtc cgcgaggacg tgctcgtcat gccgctgatc ggatcgatcg accccgagcg
      841 cgggcgacag atcctcgaga cgctgacgca cctcggcggg cgcacccacg tgcgcacggt
      901 gatcctcgat ctcaccggcg tgcgctcggt cgacaccgcg tccgcctcgg ccctgatcaa
      961 cgccgccatg gcggtgcgcc tgcgaggcgt acgtccgctg ctgaccggca tccagccggc
     1021 cgtggccgcc accctcgtgt cgctcgcggt cgacttcggc tcgctcgagg tgttcgccag
     1081 cctgcaagac gccgtcgccc aggcgacccg ccgctaaacc cggcgtcacc ggcctcgtgg
     1141 tagccttcgc gggccatggc gaggatcgag gtgcgcacat gagtatgttc ttgcgccggc
     1201 tcggcatcga tacaccgatt atccaggcgc cgatggccgg cgtgtcgacg ccggcgatgg
     1261 cggccgcggt cgccgaggcc ggcgcgctcg ggtcgatcgc catcggcgcg gtcgacgtcg
     1321 ccgccgcccg aaacatgatc gctgcggtcc gcgccctgac ctcgcgcccc ttcaacgtca
     1381 acgtgttctg ccaccgcccg ccggtcgccg accccgaccg cgagcgcgcg tggctgagcc
     1441 gcctcgcgcc ggagctcgcc cgctacggag cgacacctcc cgcgcggctg cggccgatct
     1501 acaccagctt cgtcgaggac gacgcgatgc tggcgatgct gctggcagag cgcccggcgg
     1561 tcgtcagctt ccacttcggc ctgccctcca gcgagcggat cgccgcgctg cgagccgccg
     1621 gcatcgtcct gttcgcgacc gccaccgacc tcgccgaggc ccgggcggtc gccgccgcgg
     1681 gtgtcgacgt cgtcgtcgcg cagggcatcg aggccggtgg tcaccgcggt gtcttcgatc
     1741 cagccgcgcg cgacgacggc ctcggcaccc tggcgctcgc tcgcctgctc gtgcgcgcgc
     1801 tcgacatccc ggtgatcgcc gccggtggga tcatggacgg ggcgggcatc gccgcgtgcc
     1861 tgcgcctcgg cgtcgccgcc gtgcagctcg gcaccgcctt catcgcctgc cccgaatctt
     1921 cggccgacgc cggctacctg gccgcgctcg cgagtcaggc cgcgcatcac accgtgatga
     1981 cgacggcgat ctccgggcga cccgcgcgct gtctgcccaa tctcctcacc gcgctcggcg
     2041 agcgcgtcac agcggacgag gtccccgact acccgatcgc ctacgacgcc ggcaaggcgc
     2101 tgcacgccgc ggccagggcc catggtgaat tcggctatgg agcccagtgg gccggccagg
     2161 gcgccccgct ggcccgttcg ctgccggcgg cggagctgat cgcgattttg caagcggagc
     2221 tcgccgaggc gcaacgatag tcaggcgcgc tcgacctcgg gtaacacacc aaagtcgacc
     2281 gcgagcgcga ccgtgcggcc ctcgtcgtcg acgcccgcga gcacctcgta cgcgccgtcg
     2341 ccgagccccg aggcgaacac ggccgcctgg tgggcgccgc cgagcgcgag caccccccaa
     2401 ctgcctgacg agcgcccggg gccggcgctc gcgcgcagca ccgggtcgag cacgtcgatg
     2461 taatgattac ggtccgggtg ggtcgtacga aacgcggcga gcgaggcccg cagcgcgtcg
     2521 cgggagccga tgctcataaa gcaggcgagc ccggcgtcga cgaagaagcg gtggcccgtc
     2581 gaccgccagc gggtcgcgcg tgtggtcgag aggtcgagcc gcgcgaccgc gacccggcgg
     2641 ccccaggggt cgagctccgc gaggagcagg cgcacggggt agcgccccgg cggcgccccg
     2701 gtcaggatcg gtcgcacctc gagctcgtag gggtcgcaga cgatcaccat gcccgcgggg
     2761 atatcgatga cgccgacctc gacctgcgag aggcgcacgc ccgcgtactc gtgagcggtg
     2821 aacagcgcct cgaggtcgag cggcgcgcgg ccgatcaggc cgcgctcacg caggcgagcg
     2881 acccgcgcgc ggtcgggctc gtcgaggctc tgcagcagct tcgagaccgc ccacgtcgcc
     2941 aggtgggggc gcagcggctg gccgccgtcg gtcagggcgc cgtcctcacc gcgagcatcg
     3001 atctcgtcgg cccagcgctc gagcagcgcg agcaggtagg cgggcaccgg cgccgcggcg
     3061 ccggcgagcg cgtcgacgtg ggccgcgagc atcggctggc gcaccgcgaa ggtgtcgagc
     3121 tcgtgcagcc gcatcgcgtc agcccgcgag atcgggccga gcccgtgccg ggccgcgaag
     3181 ccctcggtca gcagccgcgc cagcgcctcg cccagcgcgt ccggcgagcc gagcacgccg
     3241 acgaagtcct cgctcgccag ccatgacgcc gcgagcaacc cgaacggcgg gaagctgtcg
     3301 ggatacacga agtcccagtc gagcgagcgc gacgccacgc gcagccccgc gaggaagccg
     3361 gcgaggcgcg cctcggaggt ccccgcgggc accggcgatc cgggcaactc ccaggagcgc
     3421 atgtcgagca gctcgtcggg cgggtcgaac agcatcgccg tgaagtgcac gacctcgccc
     3481 tcgcccgccc ggaagtcgag ccgcacgcgg cggccgtcgc tcgtgcaggc ctcggcgagc
     3541 gcgtcgttga ggagctccag ggtcgactcg tgcgacatgc tgcgcggctt gtaacggtgg
     3601 cccgcggcgc gcgtcaacgg gggccccggg ccacaagcgg gcatcaccgc actaccccgt
     3661 gagcgatgcc acgagcgatg acatgagcgc gagccctcgc gtgcaactac ggtgcagccg
     3721 aacggatcca actgcgccag cacatgcgcc gccgtgttca ccctcccctc aggtcattca
     3781 tgcgatccac cctcgtcccg ctcctccctc tgatcctctt cacgaccgcc tgtggcgaca
     3841 acggcggcgc gagcgagacg ggcagcgaca ccggcaccag caccgccccg ggcaccggca
     3901 ccgacccggg caccgacacc gacccgaccg gcggcgtcgc ggcgggcccg acctactggg
     3961 acgatgtcgc gccgatcttc ttctccagct gcgtcacctg ccaccaggcg ggcggcgtcg
     4021 gcccgttcgt gctcgacagc tacgccgagg ccaagaagtg ggcgcccgcg acggtggcct
     4081 cggtcgaggc gcgcatgatg ccgccgtggc tggtgaccga cgacggcacc tgcgggacct
     4141 tccaggactc gaaggcgctg gcgcaggcgg acatcgacac gatccgcgcg tgggtcgacg
     4201 ccggcacgcc cgagggcacg ccgcgcagcg acctccaggc gcccgcgccg gagacgctca
     4261 ccgacgcgat cgagttcaag accccgaagt ttagcccggc gatccagggc ggtgcgctgg
     4321 ccgagttcga cgagtaccgc tgcttcaagg tcgacaccaa gctcgccgcg gaccgcttcc
     4381 tcaccggcta cagcgtgaag cctggcaccc cggcgatgat ccaccacgtc ctggcgatca
     4441 ccgtcgaccc cgagcagacc ggcgccggtg gcaagaagaa cagcgagatc atcgcggcgc
     4501 tcgacgccga gtcgcccgac cgcgacggct ggccgtgctt cggcgccgcg ggtgacgggg
     4561 tcaactcggg cggcatcccg gtgtcctggg cccctggtca gggcgtcacg tcgctgcccg
     4621 agggcatcgg ctttcgcatc aagaaggacg actacctgat ccttcaggtc cactacaacc
     4681 tcgtcgaccc ggcgctcgag ggcatcgagg atcagacctc gctgcagatc cgcctcgccg
     4741 acacggtcga gcgcgagggc ttcttcaacc tgcccgacga cttcctcggc tcgctgttct
     4801 ccggcacccc cgaccagctc gagcccggca aggccgcggt cacctacacc ttcgaccaca
     4861 gcctcgagta cctgcccttc ctcggcgcct cgacggccga cctctacggc atcttgccgc
     4921 acatgcatca gcgcggccgc aagttccgcg tcgagctgat cgaggacggc ggcgccccgc
     4981 agtgcgccac cgacgtcaag gcgtgggact tcaactggca gctgttctac ttcttcgaca
     5041 agcccctgcc gctgaccacg aagtccaagc tgcgcgtcac ctgcgagttc gacacgcaga
     5101 gcgcgaccga gccggtgctg cccggctggg gtacgcagaa cgagatgtgc ctcgcgggcc
     5161 tgttcatcgt cccgaagtga tacgcgcata cgcgcgctcg tgcgccggag gcccgcggac
     5221 cacgtctcgc gggcctcgcc ataactctcg tgccaccgcg tcgcaggcgc gatatctcca
     5281 cgacgccact gttatggtgg tgtcgtgaaa gcctcggtgt gtccgcagcg aacgctgacc
     5341 ctgaccctgc tgtgcgcgat cggctgctcg gggggtgacg cggccaccag cggcaccagc
     5401 ggcaccgccg acgacccgag caccggcgca aacaccccga ccaccggcgc gagcgtcgac
     5461 ccgacgaccg gcaccggcga gccgccgacc accggccccg acgacgacac cggcgacacc
     5521 ggcgagccct ccgagccgcc cccggagccc tcgggcaacc agctcgacca gaacaagctg
     5581 tttacctgcg tcggcgcgcc ggcgatgccg cccgcggaca ttcgcctgct cgaccgccac
     5641 gagtggacgc gcaacgtcgg cagctgggcc gacagcgacc tcgcccgcaa cccgctgttc
     5701 gcccgccccg accaccgcta ctcgagctac ggcgacggtg aggcgctcga cccgagcgtg
     5761 ctcggtttgt acctcgacgt cgtcggcggc gcgggcgagg cgtgggccag gggcaagtac
     5821 gagcccggcc gcgtacgcac cgtgatcgag gaccccgagg cgcagtgctt ttttaaagat
     5881 gccgcgccct cgaccgagtg tgtgcgctac ttcgtgcgga ggtacctcga gcgcggcgcg
     5941 atgtaccgcc cggccagcgc cgagcaggtc gacgcgctcg tcgagttctc agaggccacc
     6001 ctcgacggcg aggccgacat cgacgcccga ccggcgacga tcaagcggat cgccgccgcc
     6061 gcgtggatga gctcgggcgc gttgcaccgc ccggagctcg gcgagggcgc gcccgacgag
     6121 cacggccgcc tgcgcctcgg cgactgggag ctcgcgcagt cgatcgccta cgccctgtcg
     6181 cgcgggccgg ccggggcccc gagcgtcaat cgcagcttca acgacggctt ctcgaagggt
     6241 gacatcgacg gcgacctcgg cggcttcttc cacgccgcga ccgacggcag catccaggac
     6301 cccgccgtcg tcgccgagct ggtgcgcgag tacgtcggcg gcctcgacga gctgcgcgcc
     6361 gacctcgtgc tcgagccccg cgacggccgc tggtgggaga accgcggcga gtactggatg
     6421 gcgatcggcg tgcgcggctt cttccgcgag tggctcgact acggcgagct cgcggaccag
     6481 ccgcccaagg tcgaggtcgc cgcgacctcg gcgtggtccg gtgccaccgt cgagaacagc
     6541 taccagaacc agatctccgg gttctacggc tacgagagca cgctcgtgca gcagctcgac
     6601 gacatgatcg cccgcatcgt cgccgacgac aaggacgtgt tcgccgccct gctgacctcg
     6661 cgcaagttct acaccccggc gaccgccggc tatcaggagg gcgagacctc gatctggaag
     6721 tcgaccgccg aggcgaaccg cgtctacaac gtgcaagggg tcaccgcgca gacccgcgag
     6781 gcccgctgga tcgacctgcc ggaggccgag cgcgcgggcg tgctgaccca cccggcctgg
     6841 ctcggcgcgc actcgctgtc cttcgagaac gaccccaacc tggtccaccg cggcaagtgg
     6901 atccgcgagg agctgctgtg caacgacctc ccggacctgc cgctcaacgt cgacgcgatg
     6961 ctcccggagg cctcgaagtc gcagtcggcc cgccagcgga tctccgagaa gatcgacgcc
     7021 gacccgtact gcagcggctg ccacagcttc atgaacccgc tcggctatcc gttcgagatc
     7081 tacaaccacg ccggcttcct gcggatcgaa gatcacggcg ctccgccgaa cggcaccagc
     7141 acgctcgtca acatgccctc gcccgagctc aacggcgcgg tcaccagcgc gatcgacctc
     7201 agccagcgct tcgccggctc gccctacgcc aaacgctgct tcatgcgcca gacctttcgc
     7261 tacttcgccg gccgcgagga gacgatggcc gacgcctgca cgatggtcgc gatcgagaag
     7321 gcctacgacg acagcggcgg ctcgttcacc gagatgctca tcgccctctt taccagcgac
     7381 agcttccaga tgcgggcgcc cgattgagcc ccaggacctc gaccatgttc acacgacgca
     7441 agctcctcac gacctccggc ctgctcgcgg gctcggcgat gacgctcggc acgctcgggg
     7501 ccttcgggct cggcgggctc ggcgggctga cgctcggcag ccgccgcacc cgcgcggccg
     7561 agggtgaccc gcgccggatc gtcatcttcc tcgagggcaa cggcacccgc ccggtgtgca
     7621 tgcgcgaccc gctgaccagg gagacgctcg agggcttcgc cggcaagccg atcgagagca
     7681 accgcgacta cggccacgat gacccggtgc tgatcgccaa ggccccgctc gccgaggcgc
     7741 gcgcgctcgg gtcgctcgcc ggcgcgcagg accagatctc gctcgtcgag cgctcggcgc
     7801 tggtgctcgg cctgtcggcg acctacctcg ggggcgggca ctcgagcgac ttcggggccc
     7861 tgagcgccga caaggccctc ggcggtccgg cggccgccac gatcgacacc gtgctcgccg
     7921 gcatccccga ggtccgcggc accacgccct tcgacgtcgt gcgcgtcggc gtcgcggccg
     7981 cttcgacgcc gctcaattac tcgagctgcg cgttcgcaaa gggcaagccg gcgccgatcc
     8041 tgatcagccc ggccgacgcc tacgcctcgc tgttcggctc ggtcgcgtcg ggccaggcgg
     8101 gcgaggactt ccaggcccgc aaacaattgc tcgagtttgc gatcgaagac ctcggccgcg
     8161 agctcaaggg cttcgccggc tcgtcgcgcg cgcaggccaa gctcgagacc tacgaggcct
     8221 cgctgatggc gatgctggcc cgcaacgagc agatcctcgg catgaagggc aagctgctcg
     8281 cacacaagcc cgcgggcccc ggcgagctcg accccgacct ctacgcctcg atcgacccgc
//
LOCUS       DASWNJ010000128         1652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_673547, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000128 DASWNJ010000000
VERSION     DASWNJ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673547"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VGB85_02360"
     CDS             complement(<1..558)
                     /locus_tag="VGB85_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132885.1"
                     /translation="MQRTRLVALAFAAAFVTSLACDSKPEAAPAKAEPAKTGPEPATT
                     EPATTEPAKPETAPRLAPAAANTAGPAFFAVDKVGVVKLDDDGFSVVAGSPNTLQKDM
                     QVGPDGALYMLGFEDIYRLEGAGMVSIARGGFDVLGASPDHLAVAPGGDLWVTTFKGV
                     ARWDGKAWTTEDKAKIGAGDDLLEAV"
     gene            complement(590..>1652)
                     /locus_tag="VGB85_02365"
     CDS             complement(590..>1652)
                     /locus_tag="VGB85_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132886.1"
                     /translation="AAALPAPADLMPAQHARSAAGPASDWLRGPTFDLGLIGGVLVLA
                     LVLGGLASASPRLFTAVLVLDLWLLAYPHVASTFTRVAFDRASARRHWFLLLALPPIV
                     LLATAGMYRVGGFVALSSLYYYWQSWHYTRQSHGIARAYHRAAGLRVPDRLDDAVILA
                     FPLWGVLHRVYQQPGEFYQVPLWTPAIPGALVVLAGSAALGLLLVWSLRYVRAARSSP
                     PRSLGHALFVLSHVAITVVSYLVISDVTRGWLFINIWHNAQYLLFVWAMNARRFAAGI
                     DPRRPFLSAICQPTRVGHYALTCLALSTGFYLLLGQTPEAPAWSPLLPLLLVCHMAVN
                     FHHYLVDAVIWGSRARSRP"
BASE COUNT          286 a          533 c          639 g          194 t
ORIGIN      
        1 gaccgcctcg agcaggtcgt cgccggcgcc gatcttggcc ttgtcctcgg tggtccaggc
       61 cttgccgtcc cagcgcgcca cgcccttgaa ggtcgtcacc cacaggtcgc cgccgggggc
      121 gaccgcgagg tggtccggcg acgcgccgag cacgtcgaac ccgcccctgg cgatcgacac
      181 catgccggcg ccctcgaggc ggtagatgtc ctcgaagccg agcatataaa gcgcgccgtc
      241 ggggccgacc tgcatgtcct tttgcagggt gttcggcgag cccgcgacga cggagaaacc
      301 atcatcgtcg agcttgacga cgccgacctt gtcgaccgcg aagaaggccg gcccggcggt
      361 gttggcggcc gcgggcgcga gcctcggcgc ggtctcgggc ttcgcgggct cggtcgtcgc
      421 gggctcggtc gtcgcgggct ctggcccggt cttcgcgggc tcggccttcg ccggggccgc
      481 ctcgggcttc gagtcacagg cgagggacgt gacgaaggcg gcggcgaagg cgagggccac
      541 gaggcgagta cgctgcatcg cgcgaggatg cgagagtacg ccgcacgcgt caaggccggg
      601 agcgcgcgcg ggacccccat atgacggcgt cgacgaggta gtgatgaaaa ttgaccgcca
      661 tgtggcagac cagcagcaat gggagcaggg gcgaccacgc cggcgcctcc ggggtctggc
      721 cgagcaggag atagaacccg gtcgagagcg ccaggcaagt cagcgcataa tgaccgacgc
      781 gcgtgggctg gcagatcgcc gagaggaacg gacggcgcgg gtcgatcccg gccgcgaagc
      841 ggcgggcgtt catcgcccac acgaacagca gatactgcgc gttgtgccag atattgataa
      901 acagccagcc gcgggtgaca tcggagatca ccagataact gacgaccgtg atcgcgacat
      961 gcgacagcac gaacagcgcg tgaccgaggc tgcgcggggg ggacgaccgc gcggcgcgga
     1021 cgtatcgcag gctccagacc agcagcaggc ccagcgcggc cgagccggcg agcacgacca
     1081 gcgcgccggg gatcgccggg gtccacagcg ggacctgata aaattcgccg ggctgctgat
     1141 atacccggtg cagcacgccc cacagcggga atgcgaggat cacggcgtcg tcgaggcggt
     1201 cgggcacgcg caggccggcg gcgcggtggt acgcgcgggc gatgccgtgg ctctgacgcg
     1261 tgtagtgcca ggattgccag tagtagtata acgagctgag cgcgacgaat cctccgacgc
     1321 gatacatacc cgcggtggcg agcagcacga tcggcggcag ggcgagcagc aggaaccagt
     1381 gccgtcgcgc gctcgcgcgg tcgaaggcga cgcgcgtgaa ggtcgaggcg acgtgcgggt
     1441 aggcgagcag ccacaggtcg agcacgagca ccgcggtgaa caagcgcggg ctcgccgagg
     1501 cgagcccgcc gagcaccagc gcgagcacga ggaccccgcc gatcaggccg aggtcgaagg
     1561 tggggccgcg cagccagtcg ctcgcgggcc cggcggcgct gcgggcatgc tgggcgggca
     1621 tcaggtcggc gggggcgggt agggccgcgg cg
//
LOCUS       DASWNJ010000129         1912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_108700, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000129 DASWNJ010000000
VERSION     DASWNJ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108700"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VGB85_02370"
     CDS             <1..340
                     /locus_tag="VGB85_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132887.1"
                     /translation="ITGPAAIRRARTTMDSTSLTSYISAKFSLHVGIPEAELFGEDLS
                     LATIIHRSERLTNSVDLMEAFARTANALRKEHGVRVRLPALSLETPISTVLAVFIKEA
                     EAQAGDGVAA"
     gene            337..1287
                     /locus_tag="VGB85_02375"
     CDS             337..1287
                     /locus_tag="VGB85_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132888.1"
                     /translation="MSAQIVTAPAVAVRRARRPTDSKPAIIDELHSALTVEGMSLIDI
                     TWEQHRMHEHRRWSALELLAAVDMERLSEADRYRVWNAGRAELTTKPGADRVARLADR
                     ECHEWRPRDPVVANIMQALSTWSRYWNEEEAYHETAFNQLAHLVGLPAVPDETFIEFR
                     KVFPDDDMLRTLTLLAISEITAAINYAECARLAEDPGLRALFKQVGADEIQHMNYFIA
                     FAKALVDSGHWHAKEAFAVAHFFVREGGDLYGSRRAKIEARGTHVNWWDHVEHGPASD
                     TPDAIDRKRGLVYQALRRITGISVDSVQAVEDTWMDLVGC"
     gene            1281..>1912
                     /locus_tag="VGB85_02380"
     CDS             1281..>1912
                     /locus_tag="VGB85_02380"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY0132889.1"
                     /translation="MLIALGHDLQKISELAARDDLLAPGAMFTAAECERLVRSSSPLH
                     SLAGAFASKEALFKALPAVEGGFWTDLEIVHDRRGAPQLRAHGAFAALLAREGWRVQL
                     TVSHSGDYASAIAAVVAGPTARAPAPSENPMQTTATILDLRIRPNDLDSLGHVNNAVV
                     LEYFEAGRWAWMDARGIGRRGKVVPVVSRIEVDYRRELTGPELQVHTTLLG"
BASE COUNT          307 a          706 c          636 g          263 t
ORIGIN      
        1 cattactgga cccgcggcga tacgccgcgc gaggacaacc atggactcca catcacttac
       61 ttcgtacatc tccgcgaaat tcagccttca cgtcggcatc ccggaggccg agctgttcgg
      121 cgaagacctc tcgctggcga cgatcatcca ccgctcggag cgtttgacca acagcgtcga
      181 cctgatggag gccttcgcgc gcacggccaa cgcgctgcgc aaggagcacg gcgtgcgcgt
      241 gcggctgcct gccctctcgc tcgagacccc gatctcgacg gtgctcgcgg tcttcatcaa
      301 ggaggccgag gcccaggccg gggacggggt ggccgcgtga gcgcccagat cgtcacggcc
      361 cccgcggtcg ccgtccgccg cgctcgtcgc ccgaccgaca gcaagccggc gatcatcgac
      421 gagctgcaca gcgcgctcac ggtcgagggc atgagcctga tcgacatcac ctgggagcag
      481 caccgcatgc acgagcatcg ccgctggagc gcgctcgagc tgctcgccgc ggtcgacatg
      541 gagcgcctga gcgaggccga ccgctaccgc gtgtggaacg ctgggcgggc cgagctgacg
      601 accaagccgg gggccgaccg cgtcgctcgt ttggccgacc gcgagtgcca cgagtggcgg
      661 cccagggacc cggtcgtcgc caacatcatg caggcgctga gcacgtggag ccgctactgg
      721 aacgaggagg aggcctacca cgagaccgcc ttcaaccagc tcgcgcacct cgtcgggctg
      781 ccggcggtcc cggacgagac cttcatcgag tttcgcaagg tcttcccgga cgacgacatg
      841 ctgcgcaccc tgaccctgct ggcgatctcg gagatcaccg ccgcgatcaa ctacgccgag
      901 tgcgcgcgac tggccgagga ccccggcctg cgcgccctct tcaaacaggt cggcgccgac
      961 gagatccagc acatgaacta cttcatcgcc ttcgccaagg cgctggtcga cagcggccac
     1021 tggcacgcca aggaggcgtt cgcggtcgcg cacttcttcg tgcgcgaggg cggcgacctg
     1081 tacggcagcc ggcgcgccaa gatcgaggcc cgcgggaccc acgtcaactg gtgggaccac
     1141 gtcgagcacg gccccgccag cgacaccccc gacgcgatcg accgcaagcg cgggctcgtg
     1201 tatcaggcgc tgcggcgcat caccggcatc tcggtcgact cggtgcaagc cgtcgaggac
     1261 acctggatgg acctcgtcgg atgctgatcg cgctcgggca cgacctacaa aagatctccg
     1321 agctcgccgc gcgcgacgac ctgctcgcgc ccggagcgat gttcaccgcc gccgagtgcg
     1381 agcgcctcgt gcggtcgagc tcgccgctgc acagcctcgc cggcgccttc gccagcaagg
     1441 aggcgctttt caaggccctg ccggcggtcg agggcggctt ttggaccgac ctcgagatcg
     1501 ttcacgaccg tcgtggcgcg ccgcagctgc gcgcgcacgg ggccttcgcg gccctgctcg
     1561 cgcgcgaggg ctggcgcgtg cagctgacgg tcagccacag cggcgactat gcctcggcga
     1621 tcgccgccgt cgtcgccggt ccgaccgcgc gggcccccgc gccgagcgag aaccccatgc
     1681 aaaccaccgc gacgatcctc gacctgcgca tccgccccaa cgacctcgac agcctcggcc
     1741 acgtcaacaa cgcggtcgtg ctcgagtact tcgaggccgg ccgctgggcc tggatggacg
     1801 cgcgggggat cggccgccgc ggcaaggtgg tgccggtcgt ctcgcgcatc gaggtcgatt
     1861 accgccgcga gctcaccggg cccgagctgc aggtgcacac caccctgctc gg
//
LOCUS       DASWNJ010000130         4497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_285042, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000130 DASWNJ010000000
VERSION     DASWNJ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285042"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VGB85_02385"
     CDS             <1..496
                     /locus_tag="VGB85_02385"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0132890.1"
                     /translation="SEARRRVDLGPYVREVVASLAPMYRRTPHRVLVEVKAEVEISTL
                     VGAVSQVCTNLVQNALMHAFPDGNAGTITLEVDRHGEGGALLVCADDGVGMAPQVLRR
                     IYEPFFTTQRGRGGSGLGMHIVHNLVTELLGGAIEIASTPGQGTRVSIRLPADRSPHD
                     RDDR"
     gene            477..2246
                     /locus_tag="VGB85_02390"
     CDS             477..2246
                     /locus_tag="VGB85_02390"
                     /inference="COORDINATES: protein motif:HMM:NF023277.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3369 domain-containing protein"
                     /protein_id="HEY0132891.1"
                     /translation="MTAMTDDNDLMVFAEAGAEPTPAPPSTRAWKLLIVDDEPEIHEI
                     TRLALADLRIDGHPLAILSVHSATSAKVVLQAHPDIAVILLDVVMESEDAGLELAREI
                     RDELRNSRVRIVLRTGQPGHAPEQRVMLDYDINDYRDKTEVTAQRLITTIIGAVRSYR
                     DLCTIESHKLGLEQVVRSSAALFERQSVDQFITGVLHQLSALVSPQESAMFFQGKDLG
                     VADTAPTVVAGTGRFTAHVGKPVSHVVEEAVWEDINHLLRSRTPVLRPAYNIFGIFRL
                     TRDEDTWAAVILEGLGELSAWDQRLVELFCQNASVALENHRLSRRQSALMQAFERFVP
                     RQLLTLLGRADATQAALGDQIQREMTVVFVDLRAFTSRAEQQSPAATFEFLNDYFAAI
                     VPEIHGNGGVVDKYLGDGLMALFPDTSESAVRGALGVVERSRELGTGVGVGIHVGPVT
                     LGLVGAAGRMESTVVSDAVNIAARIERLTRRFGVDLLVSHAVREQLPPAMQDDTRPLG
                     DYVVPGKRQPVHIHEVFAGDGPELRAAKRASREAVAAAVARMTAGDLAGAADELAVLA
                     AACPNDQVLPALIDECRRREGRA"
     gene            2318..3049
                     /locus_tag="VGB85_02395"
     CDS             2318..3049
                     /locus_tag="VGB85_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132892.1"
                     /translation="MRLPCPTLLTVALLTLACQGAPATNTGDAGKPAAPAAPTAPASP
                     TAPPTSHGTPNPHGADPHAGMQAGAPVVRAPVNPGPVTPSGQVRAETLAELSFSVPTE
                     WIRKPGSNAMRLAEFTLPGPGGEATLIASRFAGGGGDAASNVNRWKTQFTSADGSPIT
                     EVKEQTSERAPLKVTTVDITGTNIAAVTPGAPERYNEPNSRLLGLIVEGVGDPVFFKV
                     VGSAQTLDVWEPAFKRFADSVTASK"
     gene            3062..3949
                     /locus_tag="VGB85_02400"
     CDS             3062..3949
                     /locus_tag="VGB85_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132893.1"
                     /translation="MRGRPSPIVLTATLGLACTKPNPLFGDESTTATTTAPATSDPTT
                     GAATADLSLGTGTPTTGESSATSEAITGSGTTSGTSTGHESTTTTGTTGDDGPPATCA
                     EARDLGMDESGVITLAVPEQPGATIDVWCEQKLAGGGWLLVGRSAPTDTPPAFGWGVA
                     TGDVEDDSQPYSLDLLTYKFPSSELLIGNYTQGKQPGGNMYQLSAPEGFPFAFAISTG
                     MTSAPQTVLGPCTPDGGPSMLRWIGYTALDDRFRLRDIPENDANDVYGLFATGFHLGG
                     GDCSSAGKLDGTQGMILVR"
     gene            complement(3951..4394)
                     /locus_tag="VGB85_02405"
     CDS             complement(3951..4394)
                     /locus_tag="VGB85_02405"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEY0132894.1"
                     /translation="MSRPKTRASLEPLREVIRDAGLRSTGPRVAVLEHLQTTKVPLSH
                     GELVEALAGHGYDAATIYRNLIDLTRAELLSRINLGDNVWRFELRGPNKATHPEHPHF
                     VCTSCGVVTCLSQVRVRLSPAAGSTKSVVSSISEIVIKGQCTQCG"
BASE COUNT          679 a         1628 c         1582 g          608 t
ORIGIN      
        1 cagcgaggcg cgccggcgcg tcgacctcgg gccgtacgtg cgcgaggtgg tcgccagcct
       61 cgcgccgatg taccgccgca cgccgcaccg cgtgctcgtc gaggtcaagg ccgaggtcga
      121 gatctcgacg ctggtcggcg cggtctcgca ggtgtgcacc aacctggtcc agaacgcgct
      181 gatgcacgcg ttcccggacg ggaacgccgg cacgatcacg ctcgaggtcg atcgccatgg
      241 cgagggcggg gcgctgctcg tctgcgccga cgacggcgtc ggtatggcgc cgcaggtgct
      301 gcgccggatc tacgagccgt tcttcacgac gcagcgcggt cgcggcggca gcggcctcgg
      361 catgcacatc gtccacaacc tcgtcaccga actgctcggc ggagcgatcg agatcgcgtc
      421 gacgccgggc cagggcaccc gcgtatcgat ccgcctgccc gccgaccgga gcccgcatga
      481 ccgcgatgac cgatgacaac gacctgatgg tgttcgccga ggccggcgcg gagccgacac
      541 cggcgccgcc cagcacgcgg gcgtggaagc tgttgatcgt cgacgacgag ccggagatcc
      601 acgagatcac ccgcctcgcg ctcgccgacc tgcgcatcga cggtcacccg ctggcgatcc
      661 tgtcggtgca ctcggcgacc tcggcgaagg tcgtgttgca ggcgcacccg gacatcgcgg
      721 tgatcctgct cgacgtcgtg atggagtcgg aggacgccgg cctcgagctc gcgcgtgaga
      781 tccgcgacga gctgcgaaac tcccgcgtcc gcatcgtgct gcgcaccggg cagcccggcc
      841 acgcgcccga gcagcgggtg atgctcgact acgacatcaa cgactaccgc gacaagaccg
      901 aggtcaccgc ccagcgcctg atcacgacga tcatcggcgc ggtccggtcg taccgcgacc
      961 tgtgcacgat cgagtcgcac aagctcgggc tcgagcaggt cgtgcgctcg agcgccgcgc
     1021 tgttcgagcg ccagtccgtc gaccagttca tcaccggcgt gctgcatcag ctgtcggcgc
     1081 tcgtcagccc gcaggagagc gcgatgttct tccagggcaa ggacctcggc gtcgccgaca
     1141 ccgcaccgac ggtcgtcgcc ggcactggtc gcttcaccgc ccacgtcggc aagcccgtca
     1201 gccacgtggt cgaggaggcg gtgtgggagg acatcaacca cctgctgcgc tcgcggaccc
     1261 cggtgctgcg gccggcgtac aacatcttcg ggatcttccg gctgacgcgc gacgaggaca
     1321 cgtgggcggc ggtgatcctc gagggcctcg gcgagctgag cgcgtgggac cagcggctgg
     1381 tcgagctgtt ctgccagaac gcatcggtgg cgctcgagaa ccaccgcctg agccggcggc
     1441 aatccgcgct gatgcaggcc ttcgagcgct tcgtgccgcg gcagctgttg acgctgctcg
     1501 gtcgggccga cgcgacgcag gccgctctcg gcgaccagat ccagcgcgag atgacggtcg
     1561 tgttcgtcga cctgcgcgcg ttcaccagcc gcgcggagca gcagagcccg gccgcgacct
     1621 tcgagttcct caacgactac ttcgcggcga tcgtgccgga gatccacggg aacggcggcg
     1681 tggtcgacaa gtacctcggc gacggactga tggcgctgtt ccccgacacc tccgagagcg
     1741 ccgtgcgcgg ggcgctcggg gtcgtcgagc gcagtcgcga gctcggcacc ggcgtcggcg
     1801 tcggcatcca cgtcgggccg gtcacgctcg ggctcgtcgg cgccgccggg cgcatggagt
     1861 cgacggtcgt gtcggacgcg gtcaacatcg cggcccgcat cgagcgcctg acgcggcgct
     1921 tcggcgtcga cctgctcgtc agccacgcgg tgcgcgagca gctgccgccg gcgatgcagg
     1981 acgacacgcg cccgctcggc gactacgtgg tgccgggcaa gcgccagccg gtgcacatcc
     2041 acgaggtgtt cgccggtgac gggcccgagc tgcgcgcggc caagcgggcc agccgcgagg
     2101 ccgtcgcggc cgcggtcgcg cgcatgacgg ccggcgacct ggcgggcgca gccgacgagc
     2161 tggccgtgct cgctgctgcc tgtccgaacg accaggtgct gccggcgttg atcgacgagt
     2221 gccggcgccg cgaggggcgc gcgtaggacg cggcggccgg tcgcatttgt cttcgccgac
     2281 cgcgggtcct gatatcctcc gcgcgaggac ctcgaccatg cgcctgccct gccccaccct
     2341 cctgacggtc gccctgttga cgctcgcctg tcagggcgcc cctgccacga acaccggcga
     2401 cgccggcaag cccgccgcgc ctgccgcccc caccgcgccc gcgtccccca ctgcaccacc
     2461 gacatcgcat ggcacgccca acccgcacgg ggccgacccg cacgcgggca tgcaggcggg
     2521 cgcgccggtg gtgcgcgcgc cggtcaaccc tggcccggtg acgccgagcg gccaggtgcg
     2581 cgccgagacg ctggccgagc tgagcttttc ggtcccgacc gagtggatcc gcaagcccgg
     2641 gtccaacgcg atgcgcctgg cagagttcac gctgcccggc cccggcggcg aggccacgct
     2701 gatcgcctcg cgcttcgcag gtggcggcgg cgacgctgcc tccaacgtca accgctggaa
     2761 gacccagttc acgagcgcag acggtagccc gatcacggag gtgaaggagc agaccagcga
     2821 gcgcgcgccg ctcaaggtca ccaccgtcga catcaccggc accaacatcg ccgcggtcac
     2881 gccaggggcc ccggagcgct acaacgagcc gaattcgcgg ctgctcggcc tcatcgtcga
     2941 gggcgtcggc gatccggtgt tcttcaaggt agtcggttcg gcgcagaccc tcgacgtgtg
     3001 ggagcctgcc ttcaagcgct tcgccgactc ggtgaccgcg agcaagtgat acgttgaggc
     3061 catgcgtggc cgccccagcc cgatcgtcct gaccgcgacc ctcgggctcg cctgcaccaa
     3121 gccgaacccc ctgttcggcg acgagagcac gaccgccacc acgaccgcgc cggcgacctc
     3181 ggacccgacc accggcgcgg cgacggccga cctgtccttg gggacaggca cgccgacgac
     3241 cggcgagtcg agcgcgacga gcgaggcgat caccggcagc ggcacgacca gcgggacgtc
     3301 gacgggccac gagagcacga cgacgaccgg cacgaccggc gacgacggcc ccccggcgac
     3361 ctgcgcggag gcccgcgacc tcggcatgga cgagagcggc gtgatcacgc tcgcggtgcc
     3421 ggagcagccg ggcgcgacga tcgacgtgtg gtgcgagcag aagctcgcgg gcggcggctg
     3481 gctgctcgtc ggtcgctcgg ccccgacgga cacgccgccg gcgttcggct ggggcgtcgc
     3541 taccggcgac gtcgaagacg acagccagcc gtactcgctc gacctgctga catacaagtt
     3601 cccgtccagc gagctgctga tcggcaacta cacgcagggc aagcagccgg gcggcaacat
     3661 gtaccagctg agcgcgccgg agggcttccc cttcgccttc gcgatctcga ccggcatgac
     3721 cagcgcgccg cagacggtgc tcggcccctg taccccggac ggtggcccga gcatgctgcg
     3781 gtggatcggc tacaccgcgc tggacgaccg ctttcgcctg cgcgacatcc cggagaacga
     3841 cgcgaacgac gtctacggcc tgttcgccac cggctttcac ctcggcggcg gcgactgctc
     3901 gagcgccggc aagctcgacg gcacgcaggg catgatcctc gtgcgctgag tcagccgcac
     3961 tgcgtgcact gacccttgat cacgatttca gagatgctgc tgacgaccga cttggtcgag
     4021 ccagccgccg ggctcagcct gacgcgcacc tgcgacaggc aggtgaccac gccgcacgag
     4081 gtgcacacga agtgcgggtg ctccgggtgg gtcgccttgt tcgggccgcg cagctcgaag
     4141 cgccagacgt tgtccccgag gttgatccgg ctgagcagct cggcgcgggt gaggtcgatc
     4201 aagttgcggt agatcgtcgc cgcgtcgtag ccgtggccgg ccagcgcctc gaccagctcg
     4261 ccgtggctca atggcacctt ggtggtctgt aaatgctcca gcacggccac ccgtggcccc
     4321 gtgctgcgca ggccggcgtc gcggatgacc tcgcgcaggg gctcgagcga ggcgcgggtc
     4381 ttcgggcgcg acatgaccgc cggcagggta tcacggccgg ccccgaagtg tggtaatggg
     4441 gccgccgcgg agtcgcgcat gagcatcaaa tccgaccgat ggatccgtcg tatggcg
//
LOCUS       DASWNJ010000131         3342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_532062, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000131 DASWNJ010000000
VERSION     DASWNJ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532062"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VGB85_02410"
     CDS             complement(<1..202)
                     /locus_tag="VGB85_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132895.1"
                     /translation="MVRVRSFVWISVLAACQGSDGTITSASSTADTADTADTADTFTT
                     GQGETTEDNQGEVTGGSTGEASA"
     gene            complement(673..1014)
                     /gene="tnpB"
                     /locus_tag="VGB85_02415"
     CDS             complement(673..1014)
                     /gene="tnpB"
                     /locus_tag="VGB85_02415"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEY0132896.1"
                     /translation="MIPASVRIFVCTRPHDMRCSYDTLALAAQKVLGQDPRSGALFVF
                     TNKLHNRLKVLWWDHNGYCLLSKRLHQALFRLPVPPGSSDSSVVIDGRGLAELLHGVA
                     SARTSNAQNRS"
     gene            1096..1809
                     /locus_tag="VGB85_02420"
     CDS             1096..1809
                     /locus_tag="VGB85_02420"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0132897.1"
                     /translation="MTSSTSPLSGPAIAATCDVTATNDAGASASPNFDFHHVSVRELI
                     PTFAAYALHVSPLFVQADPLTGLRHREGERLRDQQRELTVQRRLLLHHEHTALIVLIV
                     LIVHQHQALLWDQIPPSGLCRHDAVDRTLTSDHEWLIAGPLARVAGLPKDLEPRISAN
                     DFLFLREAACAGAGVAMLPAFMAGPKIAAGELVRVLENTRIDAGGLALVYSAGRNPAR
                     KVIAFRDFLIATIKKDWLE"
     gene            complement(1834..>3342)
                     /locus_tag="VGB85_02425"
     CDS             complement(1834..>3342)
                     /locus_tag="VGB85_02425"
                     /inference="COORDINATES: protein motif:HMM:NF024642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HEY0132898.1"
                     /translation="PPAQRLRPLSPARVELDPAQRAAVELPVDRSLLVLGEAGFGKTT
                     VALHRLAFLARAAAAARKPFHALMLVSTPGLQRLATRMLADLDVPRVTVDTFEHWLAA
                     QARRVFPELPRRLSEHAGPDVRAFKRHPALRAVLPRIVAGTAAMREVEAGYRDDRATI
                     RDLLLHLFGDRALLAEAADAAPDALGAATIAEVLAHTKIQFGTTTERSMGHIDAERLQ
                     TLDGRPIDAGTPDADAETIDVEDFAVVFALHHRRTGSDHTSHGALSRYQHILLDEAQE
                     LAPIELELLARAIAPGGTVTVAGDERQRGDGLVAFPGWPAVLAELGRPEAHTAALTIS
                     YRCPPEIEDVARSVLDPNVAPRSLARDEHLLLTRHDNACHRDAALVDGLLALLRDDPQ
                     AHVAVVCRHIETAARVHASLARALPARWAQGGEFRFAPGIEVCTVHDAKGLEFDLVVI
                     PDLDATTWPDRPEARRALYVAATRPLHRLWLASAGPWTPLLPAWLAAYSRGA"
BASE COUNT          518 a         1147 c         1199 g          478 t
ORIGIN      
        1 ccgccgacgc ctcgccggtg ctaccaccgg tcacctcgcc ctggttatct tccgtcgtct
       61 cgccctggcc ggtcgtgaag gtatccgcgg tatccgcggt atccgcggta tccgcggtgg
      121 atgaagcgct cgtgattgtc ccatccgaac cctggcacgc ggcgaggacc gatatccaca
      181 cgaaagacct gacgcggacc attgctcacc ctaacacagc cgcgacgggg cgacaacgca
      241 cgatcggcgt gcgccgcacc ggcagcccgt aaggcccggg aggcggtgaa gcgcgcgcgc
      301 tcaggggttc gactggatct cgtcgaacag tggcttcaca gcgtacctcg atatgtccaa
      361 agagaatggc ccgcgagcgc gggccacgac aggcgaggaa agcggcggac ctgagtctaa
      421 tcaggaccag aaggaaggtc cgccatggcc tcgcccgcca ccctgcccca ccgcgccgcg
      481 cccgtcaagc gcatcaagcc agcagcgaaa atgcgcgaaa ccggttcgcc tcctgctctg
      541 gccgaaacac gtacgcctcc gcctgccccg ctgtcacgca cgctccgcga gatcacgtgc
      601 tccgccgtca acccgccgag cacatccttc tgcccggtcc cgacgactcc gtcacagcga
      661 tctgcggccc agtcaagagc gattttgcgc attgctcgtt cgagccgacg cgacgccgtg
      721 caggagctcg gcgagcccgc ggccgtcgat gaccaccgag ctgtccgaag agccaggcgg
      781 caccggcagc cggaacagcg cctggtgcag ccgcttgctc agcaggcagt atccgttgtg
      841 gtcccaccac agcaccttga gccggttgtg gagcttgttg gtgaagacga acagcgcccc
      901 gctgcgcggg tcctggccga gcaccttctg cgccgcgagg gcgagcgtgt cgtagctgca
      961 gcgcatgtcg tgtggccgcg tgcatacgaa gatccggacc gacgccggga tcatgtacga
     1021 gcctccagcc cgtcgaggac gcgcaccagc gcgtcggcat cgacccgacc acggaggcgt
     1081 accacgacgc ggccgatgac cagctcgacc tcgccgctct cgggcccggc catcgccgcg
     1141 acctgcgacg tcaccgcgac gaacgacgct ggcgcctccg cctcacccaa cttcgacttc
     1201 caccacgtca gcgtccgcga gttgatcccg accttcgccg cgtatgcctt gcacgtgagc
     1261 ccgctcttcg tccaggcgga ccccctgacg ggtctccgtc accgcgaagg tgaacgcctc
     1321 cgagatcagc aacgtgaact gaccgtacag cggcgccttc tgcttcacca cgagcacacc
     1381 gcgctcatcg ttctcatcgt tctcatcgtc catcagcatc aagccctcct gtgggaccag
     1441 ataccgccat cgggcctatg ccgtcacgac gcggtagacc ggacgcttac gtccgatcac
     1501 gagtggctca tcgccgggcc gctggcgcgc gtcgccgggc tgcccaagga cctcgagccg
     1561 cggatcagcg cgaacgactt cctgttcctg cgcgaagcgg cgtgcgccgg cgcgggtgtg
     1621 gcaatgctgc ctgcgttcat ggccggaccg aagatcgcag ccggcgagct cgtgcgggtg
     1681 ctggagaaca cccgcatcga cgccggcggt ctggcgctcg tctactcggc ggggcgtaac
     1741 ccggcgcgca aggtgatcgc cttccgcgat tttttgatcg ccacgatcaa aaaagactgg
     1801 ctcgagtagc cgccagtcct cttcaccgca cgctcacgcg ccgcgggagt acgccgcgag
     1861 ccatgcgggg agcagcggcg tccaggggcc agcgctggcc agccatagcc ggtgtagcgg
     1921 gcgagtggcg gcgacgtaga gggcgcggcg cgcctcgggg cggtcgggcc aggtggtggc
     1981 gtcgaggtcg gggatgacga cgaggtcgaa ctcgaggccc ttggcgtcgt ggacggtgca
     2041 gacctcgatg ccaggggcga agcgaaactc gccgccctgg gcccaccgcg ccggcaaggc
     2101 ccgcgcgagg ctcgcatgga cccgggccgc ggtctcgatg tgccgacata cgacggcgac
     2161 gtgggcctgc gggtcgtcgc gcaggagggc gagcaggccg tcgaccagcg cggcgtcgcg
     2221 gtggcaggca ttgtcgtgcc gggtgagcag aaggtgctcg tcgcgcgcca ggctccgcgg
     2281 ggcaacgttc gggtcgagca cggatcgcgc gacgtcctcg atctccggtg ggcagcgata
     2341 gctgatggtg agcgcggcgg tgtgcgcctc ggggcggccg agctcggcga gcacggcggg
     2401 ccagccgggg aaggcaacga gcccgtcgcc ccgctgccgt tcgtcgccgg cgaccgtgac
     2461 ggtgccgcct ggagcgatcg cccgggccag cagctcgagc tcgatcggcg ccagctcctg
     2521 ggcctcgtcg agcaggatgt gttgatagcg cgacagcgcc ccgtgcgagg tgtgatcgct
     2581 gccggtgcgg cgatgatgca gcgcgaacac cacggcgaag tcctcgacgt cgatggtctc
     2641 ggcgtcggcg tcaggcgtgc ccgcgtcgat cgggcggccg tcgagggtct gcaggcgctc
     2701 ggcgtcgatg tggcccatcg accgctcggt ggtggtgcca aactggatct tggtatgggc
     2761 gagcacctcc gcgatggtgg cggcaccgag cgcgtcgggg gcggcgtcgg cggcctcggc
     2821 gagcagggcg cggtcgccga agaggtgcag caggaggtcg cggatcgtgg cacggtcatc
     2881 gcgatatccg gcctcgacct cgcgcatggc cgcggtgccg gcgacgatcc gcggcaggac
     2941 ggcgcggagc gccgggtggc gtttgaacgc gcgtacgtcg gggcctgcgt gctcgctgag
     3001 ccggcgtggt agctcgggga acacgcgccg cgcctgagcg gcgagccagt gctcgaaggt
     3061 gtcgacggtg acgcgcggga cgtcgagatc cgcgagcatg cgcgtggcca ggcgctgcag
     3121 gcccggggtc gagacgagca tgagcgcgtg aaacggtttg cgtgcggccg cggctgcgcg
     3181 tgcgaggaaa gcgaggcggt gcagcgcgac ggtcgtcttg ccgaatccgg cctcgccgag
     3241 caccagcagc gagcggtcga cgggaagctc gaccgcggcc cgctgcgccg ggtcgagctc
     3301 gacccgcgca ggggagagcg ggcgcaggcg ctgggcgggc gg
//
LOCUS       DASWNJ010000132         1724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_143758, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000132 DASWNJ010000000
VERSION     DASWNJ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143758"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="VGB85_02430"
     CDS             <1..1128
                     /locus_tag="VGB85_02430"
                     /inference="COORDINATES: protein motif:HMM:NF028299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system domain-containing
                     protein"
                     /protein_id="HEY0132899.1"
                     /translation="LRPLRDVLNERAGMLAARAPVAPATAESMPAAEVVEAPVTTAST
                     AFAQPSGTPAAAPLAQPCAALAPLPSVVADTADLDKFLGATGEALEQAARTLREVAPA
                     DPRAYRLLRTGLWLHLVGTPPLRPDGNTGLPGLELRDHAALAELAAASRWAGLLARSE
                     NLLPTRRLALDLQRYSAVALAGLGPEYAAAGDAIRVELRALLARLPGLAALRDRDGQP
                     LADADTQRWLAAEVLPRATASAGPSAGSDTDAAFWTDLRPRLRGETRSEALADAQRHI
                     DAGHGQQQRLRRRLALASLCDDVDPPLATHLFTALADDLERRDLDAWDPSLGVEVLAG
                     LARAQHRARDVPAMRRAFHRLARLDASAAARLLTELPEPRP"
     gene            complement(1151..1492)
                     /locus_tag="VGB85_02435"
     CDS             complement(1151..1492)
                     /locus_tag="VGB85_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132900.1"
                     /translation="MKFTLKTMTDGSGNFEKREEIDIPFYAGVALGSHADVRGRVLTP
                     EGVEVTIRADLDAADGSRRNDEVTRVVPAGRMAEIGKWRVGSGSHNLTVKGSTSPVTP
                     NTELTCEIVVD"
     gene            complement(1544..>1724)
                     /locus_tag="VGB85_02440"
     CDS             complement(1544..>1724)
                     /locus_tag="VGB85_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132901.1"
                     /translation="RLAERCTEVETGARNVEHILRSSLTPLLSQRLLTALAAGEQPTR
                     LHVESGANGWELALT"
BASE COUNT          200 a          705 c          576 g          243 t
ORIGIN      
        1 ctgcggcccc tgcgtgacgt gctcaacgag cgcgcgggca tgctcgcggc ccgggccccc
       61 gtcgcgccgg ccaccgccga gtcgatgccg gcagccgagg tcgttgaggc cccagtcacg
      121 accgcgtcga ccgcgttcgc gcagccctcg ggcacgcccg cggccgcccc gctcgcgcag
      181 ccctgtgccg cgctcgcgcc gctcccgagc gtcgtcgccg acacggccga cctcgacaaa
      241 ttcctcggtg cgaccggcga ggcgctcgag caggccgcgc gcacgttgcg agaggtcgcg
      301 cccgcggacc cgcgcgccta tcgattgctg cgcacgggtc tctggctgca cctcgtcggc
      361 acgccgccgc tgcgcccgga cggcaacacg gggctcccgg ggctcgagct gcgcgaccat
      421 gccgcgctcg ccgagctcgc cgccgcctcg cgctgggccg ggttgctcgc gcgcagcgag
      481 aacctgctgc cgacgcgccg gctcgcgctc gacctccagc gttacagcgc cgtcgcgctc
      541 gccggcctcg gcccggagta cgcggcagcc ggcgacgcga tccgtgtcga gttgcgggcc
      601 ttgctcgcgc gcttgcccgg cctcgccgcc ctgcgcgacc gcgacggcca gccgctcgca
      661 gacgctgaca ctcagcgctg gctcgcggcc gaggtcctgc cgcgcgccac ggcgagcgcg
      721 ggcccatccg ccggctccga cacggacgcg gcgttctgga ccgacctacg cccgcgtctc
      781 cggggcgaga cccggagcga ggccctcgcg gacgctcagc gtcacatcga cgccggtcac
      841 ggccagcagc agcgcctgcg ccggcgcctc gccctcgcct cgctctgcga cgacgtcgat
      901 ccgccgctgg cgacccacct gttcactgcc ctcgcggacg acctcgagcg cagggacctc
      961 gacgcctggg atccatcgct cggcgtcgaa gtgctcgccg gcctcgcccg cgcccagcac
     1021 cgcgcccgcg acgtcccggc catgcgccgc gccttccacc gcctcgcccg cctcgacgcc
     1081 agcgcggccg ccaggctgct caccgagctc ccggagccgc gcccgtgacc cccgtgacga
     1141 cgaagctcgc tcaatccacc acgatctcgc aggtgagctc ggtgttcggg gtcaccggcg
     1201 aggtcgagcc cttcaccgtc aggttgtggc tgccgctccc gacccgccac ttcccgatct
     1261 ccgccatgcg tccggccggc acgacgcgcg tgacctcgtc gttgcggcgc gagccgtcgg
     1321 ccgcgtcgag gtcggcgcgg atcgtcacct cgacgccctc gggcgtcagc acgcgccctc
     1381 gcacgtcggc gtggcttccg agggcgacgc ccgcgtagaa cgggatgtcg atctcctcgc
     1441 gcttctcgaa attgcctgaa ccgtcggtca tcgtcttgag cgtgaacttc atggaatact
     1501 cctccagtgt gtatgttgtg agggtccggg ctcgcccggc ccttcaagtc aatgccagct
     1561 cccacccgtt cgccccggac tcgacgtgca ggcgggtggg ctgctctcct gcggccagcg
     1621 ccgtcagcag gcgctgactg agcagcggcg tcagcgagct gcgcaggatg tgctcgacgt
     1681 tgcgggcgcc ggtctcgacc tcggtgcaac gctccgccag tcga
//
LOCUS       DASWNJ010000133         5136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_496473, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000133 DASWNJ010000000
VERSION     DASWNJ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496473"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VGB85_02445"
     CDS             complement(<1..211)
                     /locus_tag="VGB85_02445"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEY0132902.1"
                     /translation="MVGTDTDCGKTTVACALLRATRARGVRALPFKPAASGPEGAHAD
                     PERLVDAAGIEGLTVEEICPLRYPEP"
     gene            complement(412..1506)
                     /locus_tag="VGB85_02450"
     CDS             complement(412..1506)
                     /locus_tag="VGB85_02450"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY0132903.1"
                     /translation="MGILGFIKSQLIDIIEWTDNSNYTLVYKFPDEDHEVKNGAKLIC
                     RENQAAILVNEGQLADIFGPGTHTLSTQNVPVLSRLKGWKYGFSSPFKVDVYFVNLKQ
                     YIDQKWGTANPVLIRDPEFAVAGRPGRVRIRAFGSYNFRIADPGVFFKELVGTQGMYT
                     TDEIEGYLKRMLVGKFTQAAGKSEISVADMAGHYSVLENVVKGDIAEEFKKLGLLLTS
                     FVIENISLPPEIEKALDAAAAQSARGVDNTMAWEGMQAMRDAARQPGGGGGVMQAGMG
                     MGMGMGMGNMMGNVMAGAMPGYPQQQGYPQQQGYPQQQQYPQQGQPQQPVAEDNSLES
                     KLRKLKAALDAGLLTDEEYKAKRTKLLDAF"
     gene            complement(1549..1938)
                     /locus_tag="VGB85_02455"
     CDS             complement(1549..1938)
                     /locus_tag="VGB85_02455"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEY0132904.1"
                     /translation="MEQRQSAGGMTPEEVDVFARGLYYLASVDDHVDEREVKLIEEFL
                     QETGSELTLEDLKQRQFSFYEAVQVLEATYLRRIFVKAAIALVHSDGVYTDEERRALG
                     TVADVFGLSNAEFGDLEQEARRVSLLQ"
     gene            complement(2046..2522)
                     /locus_tag="VGB85_02460"
     CDS             complement(2046..2522)
                     /locus_tag="VGB85_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132905.1"
                     /translation="MRTVSLVFALVLPACVDAPITEAHLTPDGQLRAGLEQSSFSARP
                     QMSSRQAVQVAAVLAAQAEGALEDAAEFSASAGAVHLHLRADSLMEPRPVTFVWTHAD
                     RREEVQGVLAPTTTLTLAASHPISPDSTGRWEVEVYAADAAGAAGELLFRREFLVL"
     gene            2749..>5136
                     /locus_tag="VGB85_02465"
     CDS             2749..>5136
                     /locus_tag="VGB85_02465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5808 family serine peptidase"
                     /protein_id="HEY0132906.1"
                     /translation="MAPKRPRLLSLCLGWLALGPACGSPSGEKADAPGARGKTAKPEA
                     EPEQTPDLRAQLGLEQRSFPLTVWAAYIIQTEYFDKTRIDPRAQLVSALTYLGLHTPE
                     FFAAVTGDQATVTVGAARKEFSLAGLADLSTAADRLEEILAFAQAELRLDTDATHKLE
                     YAAINGLLAPLDPHTILLTPEEHTDLGVKTKGQFGGIGAEIREEERRIRIGRVLPGSP
                     AEKGGLRAGDLVMQIDKQSTVNLRVVEAQQLLRGPVDTKVILKVRRGEQTLTLTLTRQ
                     IIRVESVTSELLPGRIAYLKLSTFQENSGEQVRAALKNLAPKGDNGESEGLRGVVLDL
                     RDNTGGLLAQAVEILDDLVASGELVIVRSAMGREFEAADKELELPASAAVVALINEES
                     ASASEIVSGSIKYLDRGVVIGRPSFGKGTVQLVKPENLYGAELALKLTVAEYLVAGDR
                     SIQTAGVQPDLVLFPVELTNIPGVANYYDRERFDRRREFAQVAHLPSARHERPRAQQP
                     ASGLQLRYFASAPISGKASEGPAALRDPEVRIAHQVAQALAGEENVGARQAKLAAIAR
                     DLALAEDTAIATGIGATKIDWSGRVDVHEQAALELSARIVETGEIRAGAPFTLKVAVK
                     NAGATPVERVHAITECLRDELDGIELLLGRIEPGETVTRDVRLQVMGWHSSFTDALRV
                     DLHAGEPDPTPDGTATVRFDVVGQTRPHLTFDYWIVDDPALAAKAPPRPAGTLFPGEA
                     AFAVRGNGDGNLQPGEQVLIALEAHNAGPGAATDTRALVHNLSGTQVLLEEGFVQL"
BASE COUNT          816 a         1972 c         1584 g          764 t
ORIGIN      
        1 ccggctcggg gtagcgcagc gggcagatct cctccaccgt cagcccttcg atcccggccg
       61 cgtcgaccag ccgctccgga tccgcgtgcg ccccctcggg cccgctcgcc gccggcttga
      121 acgggagcgc ccgcaccccc cgcgcccgcg tcgcccgcaa cagcgcacac gcgaccgtgg
      181 tcttgccgca atccgtatcc gtcccaacca aaaagatccg cctcatattt tcttaagaga
      241 gagtatatcg cgcgcaacac cggcgtccgc ggcaagcacc actgacggca tgcccggcga
      301 tgctgtgacc gtgacggatc tccgcgacga tcgacgcggg gaccgctcac ctccgcaaaa
      361 cacgacgagg cggccggaac cccgaccgcc tcgccagctt cacatcaccg ctcagaacgc
      421 gtcgagcagc ttggtgcgct tggccttgta ctcctcgtcg gtcaacagcc cggcgtcgag
      481 cgccgccttg agcttgcgca gcttgctctc gaggctgttg tcctccgcga ccggctgctg
      541 cggctgcccc tgctgcgggt actgctgctg ctgcggatac ccctgctgct gcgggtaccc
      601 ttgctgctgc gggtacccgg gcatcgcgcc ggccatgacg ttgcccatca tgttgcccat
      661 gcccatgccc atgcccatgc ccatgccggc ctgcatcacg ccgccgccgc caccgggctg
      721 cctggcggcg tcgcgcatcg cctgcatgcc ttcccaggcc atcgtgttgt cgacgccgcg
      781 ggccgactgg gccgccgctg cgtcgagcgc cttctcgatc tccggcggca gcgagatgtt
      841 ctcgatgacg aagctcgtca gcagcagccc gagctttttg aactcctcgg cgatgtcacc
      901 cttgaccacg ttctcgagca cgctgtagtg cccggccatg tcggcgaccg agatctccga
      961 cttgcccgcc gcctgggtga acttgccgac gagcatgcgc ttgaggtagc cctcgatctc
     1021 gtcggtcgtg tacatcccct gcgtaccgac cagctccttg aaaaacacgc ccgggtcggc
     1081 gatgcgaaag ttgtagctac cgaacgcccg gatgcgcacg cgtcccggcc ggccggcgac
     1141 cgcgaactcc gggtcgcgga tcagcaccgg gttggcggtg ccccacttct ggtcgatgta
     1201 ctgcttgagg ttgacgaagt agacgtcgac cttgaatggg ctgctaaagc cgtacttcca
     1261 gcccttgagg cgcgacagca ccggcacgtt ctgggtgctc agcgtgtgcg tgcccggccc
     1321 gaagatgtcg gccagctgcc cctcgttgac gaggatcgcc gcctggttct cgcggcagat
     1381 cagcttggcg ccgtttttga cctcatggtc ctcgtcgggg aacttgtaca ccagcgtgta
     1441 gttgctgttg tccgtccact cgatgatgtc gatgagctgg ctcttgatga aacctagaat
     1501 gcccatgtca gcttccgttc ttgtcgtcgc gaggggtgcg tgaaaaggtc attgcaggag
     1561 gctcacgcgc cgagcctctt gctcgaggtc gccgaactcc gcgttgctga ggccgaacac
     1621 gtcggccacc gtaccgagcg cgcgccgctc ctcatcggtg tagaccccgt cgctgtgcac
     1681 gagggcgatc gccgccttga cgaagatgcg ccgcaggtag gtcgcctcga gcacctgcac
     1741 cgcctcgtag aagctgaact ggcgctgctt gaggtcctcc agcgtcagct ccgagccggt
     1801 ctcctgcaag aactcttcga tcagcttcac ctcgcgctcg tcgacgtggt cgtcgacgct
     1861 ggcgaggtaa tacagcccgc gggcaaacac atcgacttct tcgggggtca tgcccccggc
     1921 gctttgccgt tgttccatgg cccgcaatgc tacgagcaac caccgcccca ggccagaacc
     1981 aaaaagaaac gcccgcggtc gaaaccgcgg gcgtcgcacg actcgacgcc accaagcaag
     2041 agcgtctaga gaaccaggaa ttcgcgccgg aacagcagtt cacccgccgc ccccgccgca
     2101 tccgcggcgt acacctccac ctcccaccgc cccgtggagt cgggcgagat cgggtggctg
     2161 gccgccagcg tcagcgtcgt cgtgggcgca agcacgccct gcacctcctc acgacggtcc
     2221 gcgtgcgtcc atacgaaagt caccgggcgc ggctccatca ggctgtccgc ccgaagatgg
     2281 aggtgcaccg cccccgcgct ggcagagaac tccgccgcgt cctcgagcgc gccctcggcc
     2341 tgcgccgcca gcaccgcggc cacctgcacc gcctgacgcg acgacatctg cggccgggcc
     2401 gagaacgacg actgctcgag accggcacgg agctgaccgt ccggggtcag atgcgcctcc
     2461 gtgatcggtg cgtcgacgca ggccgggagc acaagggcga agacgagaga aacggtacgc
     2521 acgcaagaaa cttgacatgc cggtcccgtg ccactcccct tcgacgcgat ctcaactact
     2581 tgaacacccg gcgcggggcg cgacgatgcg atcgatgtcg ttctgcaatt tttagtgact
     2641 tgcggcctct gagccccgca aaggggccac gatcgcccca cggcgacgtc tgcccgccca
     2701 cgcatgaggc ccgcgggcgc ttccccgggg cgctatgctc ccgccttcat ggcccccaag
     2761 cgcccgcgcc tcctgagtct gtgtctggga tggttggccc tggggcccgc gtgcggctcg
     2821 ccctccggtg aaaaagccga cgcgcccggc gctcgcggca agaccgcgaa gcccgaggcc
     2881 gagcccgagc agacccccga cctgcgcgcc cagctcggcc tcgagcagcg cagcttcccg
     2941 ctgaccgtgt gggccgccta catcatccag accgagtact tcgacaagac gcgcatcgac
     3001 ccgcgcgcgc agctcgtctc ggcgctgacc tacctcggcc tgcacacccc cgagttcttc
     3061 gccgccgtca ccggcgacca ggcgaccgtc accgtcggcg ccgcgcgcaa ggagttctcg
     3121 ctcgccggcc tcgccgacct ctcgaccgcc gccgaccgcc tcgaggagat cctcgccttc
     3181 gcgcaggccg agctgcggct cgacaccgac gccacccaca agctcgagta cgccgcgatc
     3241 aacggcctgc tcgccccgct cgacccgcac acgatcctgc tgacccccga ggagcacacc
     3301 gacctcggcg tcaagaccaa gggccagttc ggaggcatcg gcgccgagat ccgtgaagag
     3361 gagcgacgga tccgcatcgg ccgcgtcctg cccggctcgc ccgccgagaa gggcggcctg
     3421 cgtgccggcg acctcgtcat gcagatcgac aaacagtcga ccgtcaacct gcgcgtcgtc
     3481 gaggcccagc agctcctgcg cggccccgtc gataccaagg tgatcctcaa ggtccgtcgc
     3541 ggcgagcaga ccctgacgct gacgctcacc cgccagatca tccgcgtcga gagcgtcacc
     3601 tcggagctgc tgcctggccg catcgcctat ctcaagctga gcaccttcca ggagaacagc
     3661 ggcgagcagg tccgcgccgc cctcaagaac ctggccccga agggcgacaa cggcgagagc
     3721 gagggcctgc gcggcgtcgt gctcgacctg cgcgacaaca ccggcggtct gctcgcccag
     3781 gccgtcgaga tcctcgacga cctcgtcgcc agcggcgagc tcgtcatcgt gcgctcggcg
     3841 atgggccgcg agttcgaggc cgccgacaag gagctcgagc tcccggccag cgccgccgtc
     3901 gtcgccctga tcaacgagga gtccgcgagc gcctcggaga tcgtcagcgg gtcgatcaag
     3961 tacctcgacc gcggcgtcgt gatcggccgc ccgagcttcg gcaagggcac cgtccagctc
     4021 gtcaagcccg agaacctcta cggcgccgag ctggcgctca agctgaccgt cgccgagtac
     4081 ctcgtcgccg gcgaccgatc gatccagacc gccggcgtgc agcccgacct cgtgctgttc
     4141 cccgtcgagc tgaccaacat ccccggcgtc gccaactact acgaccgcga gcgcttcgat
     4201 cgccgccgcg agttcgccca ggtcgcccac ctgccgtccg ccaggcacga gcgcccgcgg
     4261 gcccagcagc ccgccagcgg cctgcagctg cgttacttcg ccagcgcgcc gatctcgggc
     4321 aaggcgagcg agggcccggc cgcgctgcgc gaccccgagg ttcggatcgc ccatcaggtc
     4381 gcccaggccc tcgccggcga ggagaacgtc ggcgcccgcc aggccaagct cgccgcgatc
     4441 gcccgcgacc tcgcgctcgc cgaggacacc gcgatcgcca ccgggatcgg cgcgaccaag
     4501 atcgactgga gcggccgcgt cgacgtccac gagcaggccg cgctcgagct cagcgcccgc
     4561 atcgtcgaga ccggggagat ccgcgccggc gcacccttca ccctcaaggt cgccgtcaag
     4621 aacgccggcg ccacccccgt cgagcgcgtg cacgcgatca ccgagtgcct gcgcgacgag
     4681 ctcgacggca tcgagctgct gctcggccgc atcgagcccg gcgagaccgt cacccgcgac
     4741 gtccgcctgc aagtgatggg ctggcacagc agcttcaccg acgccctgcg cgtcgacctc
     4801 cacgccggcg agcccgaccc gacccccgac ggcaccgcga ccgtccgctt cgacgtcgtc
     4861 ggccagaccc gcccgcatct gaccttcgac tactggatcg tcgacgaccc ggcgctcgcg
     4921 gccaaggccc cgccgcgtcc ggccggcacc ctgttccccg gcgaggccgc gtttgccgtg
     4981 cgcggcaacg gcgacggcaa cctccagccc ggcgagcaag tcctgatcgc cctcgaggcc
     5041 cacaacgccg gccccggcgc cgcgaccgac acccgggccc tcgtccacaa cctgtccggc
     5101 acccaggtcc ttttggaaga gggcttcgtc cagctc
//
LOCUS       DASWNJ010000134         5228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_389755, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000134 DASWNJ010000000
VERSION     DASWNJ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389755"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VGB85_02470"
     CDS             <1..329
                     /locus_tag="VGB85_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HEY0132907.1"
                     /translation="RVDSAVIRLDPRPVPRGEVRDSTGFQQLVRAAFQQRRKVLSNAL
                     TSIAGRDAAQRWCATAGIDPQIRPERLGPEEFAALQRAREAEEDSGLIERRIGGPEID
                     EDAGAP"
     gene            313..1179
                     /gene="mutM"
                     /locus_tag="VGB85_02475"
     CDS             313..1179
                     /gene="mutM"
                     /locus_tag="VGB85_02475"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEY0132908.1"
                     /translation="MPELPEVESVRRGLIAAKLAAPVQSLWRSEFKLRIGKHWATENL
                     ECLHGATPGEVGRRGKYIVWTFSKGDNGDALGLVIHLGMSGRCGVATSEQPRVLHTHL
                     ALGFADGRELRLVDPRRFGGLRAGPLSEILASPPIGDLGPEPLDEGFSGEVLQARLGS
                     SARPIRDALLDQEAVAGLGNIYAVEALFLAGIHPLLEARRLHRSAWDRLAVAVITVLR
                     QGIENGGTTLKDFRNVVGEVGRNQDDLKVYGRAGLPCPRCGAPRVGFGSGGRSGAYCP
                     RDQPRPRTRLLR"
     gene            1277..1927
                     /locus_tag="VGB85_02480"
     CDS             1277..1927
                     /locus_tag="VGB85_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY0132909.1"
                     /translation="MDEVRSTGSLAAPSGSAGSGQETRTDEALMVGFRDGESRAFDVL
                     VVRHRRGLFNFLLRSVQNRSRAEEMLQEVFLRVVRSKERYEQTAKFTTWVYTIARNLC
                     VDESRRAKFRRTVSLDAPRRGADGDDGPGMLESVAADQVATDDAAAAPQLRGRIAKAI
                     DLLPDDQREVFLMRQVSGLSFKEIGEIVGAPENTVKSRMRYALDKLRTELKDFADE"
     gene            complement(1931..2605)
                     /locus_tag="VGB85_02485"
     CDS             complement(1931..2605)
                     /locus_tag="VGB85_02485"
                     /inference="COORDINATES: protein motif:HMM:NF013514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132910.1"
                     /translation="MKGHTYGVDEAGRGPILGPMALAVVVLDRKTATKLTKLGVQDSK
                     RFGAGAKAQELRANLAAEIHHCARAWACELVSVETIDEHTFRGQLNRLEQDIALQLLR
                     RLEAATDATIICDGEYLFRPLREHFPKLKAVNDGESAHVAVAAASIVAKHARDTAFAQ
                     ICERYQLEFGPVSGGGYANAHTRRFLDAYKLARGGLPPEARKSWGAEKLPPIQAPTQQ
                     LTLAEL"
     gene            complement(2602..>5228)
                     /locus_tag="VGB85_02490"
     CDS             complement(2602..>5228)
                     /locus_tag="VGB85_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEY0132911.1"
                     /translation="RRARKLIDELTGSLQPETRFVYHLLTTLAQGRRGRNERALLELH
                     KTRARLPEGTRGPLRQLLLAEGAQVSLAAESIDEAIAWLGSETEPPEGSEPDASARTA
                     RAYLALAIAAKGDRLGSEAALARVDAGDPTWDELRVRVGLCLGSGPESLVTRDLALRH
                     LQRAPEDPRRQRLWALAEAATWPSNGAAAPLEARRACINALVEAAHAAPPDLLERQLQ
                     ELAHLALRADLFPEAAVTAIRTHLRDSLAAPEELRLVNARVRVQQRDEQVMEDFVSGP
                     PPRFRGQADLGGPWGPDEVSPLRDVGLRLTVLRSQRALASAELCLQRGLAEPAQEYLV
                     EVLTEFPEHEHARALLIDLARPVESNRLEDLLTAATALLAAMPGRILGVPLGGVQEAL
                     TGVIAARERLARPLTIAIMGEFSSGKSTFINALLGEAVAPMGVLPTTSTINLFRRGPG
                     GGARVHYRDGSIATVARDEVHGFLQDLDDLEASRIRHMEIERTGARLGDAAVVDTPGL
                     NALDTFHERVTREFVEEADAIIWIFSATRGGAASEGSALKSLRADGRQVLGVLNKVDT
                     LDDDERTELVDYLHEQFGDLLLDVLPICASAALELRTRPPETDVLAQAPLQEDPFRAV
                     EAALERHFFERARELKRSLTARRLADALTHAREIVLRTADALDAKADAAAVGDASDPA
                     QLEFRLVNFADTVYGQILALDDLLTRECLALGILRAGATPTRAFLAPQDATYLSAVLR
                     DSILRTLQTDLTDLTQTPGTEALTDVLTSRLIPWAQGFLDSLDSSGFVATLVMEHGPN
                     VAKGEAALRERYRVALQPVAASWRKFIRGLFRHIRQAQRQARHTAASAPRAEALRLRT
                     TTVASLDAVLGSLERVAP"
BASE COUNT          714 a         1621 c         2120 g          773 t
ORIGIN      
        1 cgcgcgtcga ctctgcggtg atccggctcg acccgcgacc ggtgccgcgc ggggaagtgc
       61 gtgactcgac cggctttcaa cagctggtgc gcgcggcgtt ccagcagcga cgcaaggtgc
      121 tcagcaacgc gttgacgtcg atcgccggac gcgacgcggc gcagcgctgg tgcgcgaccg
      181 cgggcatcga tccgcagatc cgtcctgagc gcctcggacc cgaagaattc gcggcgctgc
      241 agcgggcgcg cgaggccgag gaggacagcg gtttgatcga aagacggatc ggcgggccgg
      301 agatcgacga ggatgccgga gctccctgaa gtcgagtccg tgcgccgcgg tctgatcgcg
      361 gcgaagctgg ccgcgccggt gcagtcgctg tggcgcagtg aattcaagct gcggatcggc
      421 aagcactggg cgaccgaaaa cctcgagtgc ctgcacggcg cgacgccggg tgaggtcggc
      481 cgccgcggca aatacatcgt ctggactttt agtaagggcg ataacggcga cgcgctcggt
      541 ttagtaattc acctcgggat gagcgggcgc tgtggggtgg cgacgagcga gcagccgcgt
      601 gtgctgcaca cgcacctcgc gctcggcttc gcggatggac gggagctgcg gctggtggac
      661 ccgcgacggt ttggtgggct gcgcgccggg cccctttcag agattctcgc gtcgccgccg
      721 atcggtgatc tcgggcccga gccgctcgac gagggatttt ccggcgaggt gttgcaggcg
      781 cggctgggga gctcggcgcg accgatccgg gacgcgctgc tcgaccagga ggcggtcgcg
      841 gggctcggga atatttatgc ggtcgaggcc ctgttcctcg cggggatcca tccactgctc
      901 gaggcgcgtc ggctgcaccg cagcgcgtgg gatcggctcg ccgtggcggt gatcaccgtg
      961 ttgcggcagg gcatcgagaa cggcgggacc acgctcaagg attttagaaa tgtcgtcggc
     1021 gaggtcggca ggaatcagga cgacctgaag gtgtacggtc gggcgggcct gccgtgcccg
     1081 cgctgtgggg cgccgcgcgt cggcttcggg tcgggcgggc gcagcggggc gtactgtccc
     1141 cgcgaccagc cgaggccgcg gactcgcctg ctgcgctgaa gggccgtcgg gcggcgtcgg
     1201 cgagcgcgcg aaacagaccg cttgccaggg attcgggcat gggcgcacac ttgcacggcc
     1261 cgaaggcgcg ggcgccatgg acgaggtccg cagcactggc tcactggccg ccccctctgg
     1321 atctgctggt tcgggacagg agacgcggac cgacgaggcg ctgatggtcg gctttcgcga
     1381 cggcgagtcg cgggccttcg atgtgctggt cgtccgccat cgtcgcggct tgttcaattt
     1441 tctgctgcgc agcgtccaga accgcagtcg cgcggaggag atgttgcagg aggtgttcct
     1501 gcgggtcgtg cgttcgaagg agcgctacga gcagaccgcg aagttcacga cgtgggtgta
     1561 cacgatcgcc cgcaacctct gcgtcgacga gagccggcga gcgaagtttc gccgcacggt
     1621 ttctctcgac gcgccgaggc gcggcgcgga cggtgacgat ggtccgggga tgctcgagtc
     1681 ggtggcggcg gatcaggtcg cgaccgacga cgccgccgcg gcgccgcagc tgcgcgggcg
     1741 gatcgccaag gcgatcgacc tgctgccgga tgaccagcgc gaggtgttcc tgatgcgtca
     1801 ggtcagcggg ctgtcgttca aggagatcgg ggagatcgtc ggtgcgccgg agaatacggt
     1861 gaagagtcgc atgcgctacg cgctcgataa actccgcacc gagctcaagg acttcgccga
     1921 cgagtgatga tcaaagctcg gcgagcgtga gctgctgcgt cggtgcctgt atcggcggca
     1981 atttctcggc gccccagctc ttgcgcgcct ccggcgggag gccgccgcgg gccagcttgt
     2041 aggcgtcgag gaagcgtcgc gtgtgcgcgt tggcgtagcc gcctcccgag accgggccga
     2101 actcgagctg gtagcgctcg cagatctgcg cgaaggcggt gtcacgcgcg tgcttggcga
     2161 cgatcgaggc ggcggcgacg gcgacgtgcg ccgactcgcc gtcgttgacg gccttgagct
     2221 tcgggaagtg ctcgcgcagg ggacggaaca ggtactcgcc gtcgcagatg atcgtggcgt
     2281 ccgtggcggc ctcgaggcgg cgcagcagct gcaaggcgat gtcttgctcg aggcggttga
     2341 gctggccgcg aaaggtgtgc tcgtcgatcg tctcgacgga gacgagctcg caggcccagg
     2401 cgcgcgcaca atgatggatc tcggcggcga ggttggctcg cagctcctgc gccttcgcgc
     2461 cggcgccgaa gcgcttgctg tcctgcacgc cgagcttggt caacttggtc gcggtcttgc
     2521 ggtcgagcac gacgaccgcg agcgccatcg gtccgaggat cgggccgcgg ccggcctcgt
     2581 cgacgccgta ggtgtggccc ttcatggcgc gaccctctcg aggctgccga gcacggcgtc
     2641 gaggctggcg acggtggtgg tgcgcaggcg cagcgcctcg gcgcgcggag ccgacgcggc
     2701 ggtgtgtcgg gcctggcgct gggcctggcg gatgtgacgg aagagaccgc ggatgaattt
     2761 ccgccagctc gcggcgaccg gctggagggc gacgcggtag cgctcgcgca gggcggcctc
     2821 tcccttggcg acgttggggc cgtgttccat gacgagggtg gcgacgaagc cggacgagtc
     2881 gaggctgtcg aggaagccct gggcccacgg gatcaggcgg ctggtgagca cgtcggtcag
     2941 ggcctcggta cctggcgttt gggtcaggtc ggtgaggtcg gtctgcagcg tgcgcaggat
     3001 gctgtcccga aggacagccg agaggtaggt ggcgtcctgc ggggccagga aggcgcgggt
     3061 cggggtggcg ccggcgcgca ggatgccgag cgcgaggcac tcgcgggtca gcaggtcgtc
     3121 gagcgcgagg atctggccgt agacggtgtc ggcgaagttg acgaggcgga actcgagctg
     3181 ggccgggtcg ctggcgtcgc cgacggcggc cgcgtcggcc ttggcgtcga gggcgtcggc
     3241 ggtgcgcagg acgatctccc gcgcgtgggt cagggcgtcg gcgaggcgac gggcggtgag
     3301 agacctcttg agctcgcggg cgcgctcgaa gaagtgacgc tcgagcgctg cctcgacggc
     3361 gcggaagggg tcctcctgga gcggcgcctg ggcgagcacg tcggtctcgg gcgggcgggt
     3421 gcgcagctcg agcgcagcgc tggcgcagat cgggaggacg tcgaggagca ggtcgccgaa
     3481 ctgctcgtgc aggtagtcga cgagctcggt gcgctcgtcg tcgtcgaggg tgtcgacctt
     3541 gttgagcacg ccgaggacct gacggccgtc ggcgcgcagg ctcttgaggg cgctgccctc
     3601 ggaggccgcg ccgccgcggg tggccgagaa gatccagatg atcgcgtcgg cctcttcgac
     3661 gaactcgcgc gtgactcgct cgtgaaaggt gtcgagggcg ttgaggccgg gggtgtcgac
     3721 gacggcggcg tcaccgaggc gcgcgcccgt gcgctcgatc tccatgtggc ggatccggct
     3781 ggcctcgagg tcgtcgagat cctgcaggaa tccatggacc tcgtcgcgcg cgacggtggc
     3841 gatcgatccg tcgcggtagt gcacgcgggc gccgccgccg gggccgcggc gaaacaggtt
     3901 gatcgtcgag gtggtcggca gcacgcccat cggcgcgacg gcctcgccga gcagggcatt
     3961 gatgaaggtg ctcttgccgg aggagaactc gcccatgatc gcgatcgtca gcggacgggc
     4021 gaggcgctcg cgggccgcga tcacgccggt gagggcctcc tgcacgccgc ccagcgggac
     4081 gccgaggatc cgtccgggca tcgccgcgag taatgcggtc gcggcggtca ggaggtcctc
     4141 gaggcggttg ctctcgacgg ggcgcgcgag gtcgatcagc agggcgcgcg cgtgctcgtg
     4201 ctcggggaac tcggtcagga cctcgacgag gtactcctgc gcgggctcgg cgaggccgcg
     4261 ttgcaggcac agctcggcgg aggcgagggc gcgctggctg cggagcacgg tcaggcgcag
     4321 gccgacgtcg cgcagcgggg agacctcgtc ggggccccac gggccgccga ggtcggcctg
     4381 gccgcgaaag cgcggcggcg ggccggacac gaagtcctcc atgacctgct cgtcgcgctg
     4441 ttgcacgcgc acgcgcgcgt tgacgaggcg cagctcctcc ggcgcggcga ggctgtcgcg
     4501 caggtgcgtg cggatggcag tgacggcggc ctcggggaag agatctgccc gaagggccag
     4561 gtgggcgagc tcttgcaact ggcgctcgag caggtccggc ggggcggcgt gggcggcctc
     4621 gacgagcgcg ttgatgcagg cgcggcgggc ctcgagcgga gcggcggcgc cgttgctcgg
     4681 ccaggtcgcg gcctcggcga gggcccacag gcgctggcgg cgggggtcct cgggggcgcg
     4741 ttgcaggtgg cgcagggcga ggtcgcgggt caccaggctc tcggggcctg acccgaggca
     4801 caaaccaacg cggacgcgca gctcgtccca ggtggggtcg ccggcgtcga cgcgggcgag
     4861 cgcggcctcg gagccgaggc ggtcgccctt ggcggcgatc gccagggcga ggtaggcgcg
     4921 ggcggtgcgg gcggaggcgt cgggctcgct gccctcgggc ggctcggtct cgctgccgag
     4981 ccaggcgatc gcctcgtcga tgctctcggc ggcgagcgac acctgggcgc cctcggcgag
     5041 caacagctgg cgcagtgggc ctcgggtgcc ctcgggcagg cgcgcgcggg tcttgtgcag
     5101 ctcgagcagc gcgcgctcgt tgcggccgcg acggccctgc gcgagggtgg tcagcaggtg
     5161 atagacgaag cgggtctccg gctgcagcga gccggtcagc tcgtcgatca gcttgcgagc
     5221 gcggcgca
//
LOCUS       DASWNJ010000135         3144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_603086, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000135 DASWNJ010000000
VERSION     DASWNJ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603086"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VGB85_02495"
     CDS             complement(<1..298)
                     /locus_tag="VGB85_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132912.1"
                     /translation="MDRPFSDLGLADEQRRPPVFAAALLRATTGPFLDRRRTDMFARS
                     SFAAYKIWSRKSDPRGPSAAGGTNRPGWYTRELDQFFDVLPEIQKILDWDRSTRR"
     gene            complement(787..2337)
                     /locus_tag="VGB85_02500"
     CDS             complement(787..2337)
                     /locus_tag="VGB85_02500"
                     /inference="COORDINATES: protein motif:HMM:NF021352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2357 domain-containing protein"
                     /protein_id="HEY0132913.1"
                     /translation="MKIVIVDLHGHTVLVEDDGTFVLEEDRHYQVLAAGASEARLDAR
                     LLLAANPGCFVLPIDRWVGATQLQLETADGPVAARVRVRPRSEKLSEALWATLLEELE
                     AWLPGVTPGLAGGKQGAVGSEGVSATLFAEALLPLLPALEDALRVVLAHPRVADRIEP
                     EDRPLRQIRRVESSTIAWLGRHPTAGAWLHPWRAAEQIGAPPLVPATMVDATLDHPAN
                     RYIAWILRTAIARLREAADILDRRARRPDDSGAWCAARAERARTGADRLATLRDRSFL
                     RRLSPTPATEAALLVIADDIAYARLHALGRRFCSPRFRLHGAASDPAAAVRPSYSLYE
                     LWCLLAVERSLRSCLADWTWSSRNTQRLLDLASTGEGIRFDAVSPAGARLGIEFNPTF
                     YGWFNHGHHPRFSLSGERRPDLVLTLDTPGGTGRWVVLDAKYRAGRRNLADAFESLHI
                     YRDALRDEDRGGACLGGLLLAPQPDQEIQEWFAPSWRTRWGIGAVALAPGGTADELVE
                     WLLVTLGL"
     gene            complement(2334..>3144)
                     /locus_tag="VGB85_02505"
     CDS             complement(2334..>3144)
                     /locus_tag="VGB85_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132914.1"
                     /translation="CEQLGRDVEHHRAVVPVSPDWRDPTGLLGYFNALQADATYQAEP
                     ALRLVLAAAKRPDLPYFLILDEMNLARVEHYFAPFLSAMETGDRLVLHAQDEPVNGVP
                     PWVPWPRNLFIGGTINMDETTHAISDKVLDRAFTLEFWQVDLATFFERRAAAGHPRHL
                     VAETELLALHAALTPIRRHFGYRTASEILGMLDAAAAATLDAAAVAEVLDQAIFSKVL
                     PRLRGEETPALHQALTVILDHCQSRYLPRCAAKIAEMQARLRSTGLTKFWS"
BASE COUNT          493 a         1054 c         1154 g          443 t
ORIGIN      
        1 cccgtcgcgt ggatcgatcc cagtccagga tcttctggat ttctggcaga acatcaaaga
       61 actggtctaa ctcgcgcgta taccagccgg gccggttcgt tcctccggca gccgatgggc
      121 cgcgcggatc tgacttccgc gaccatattt tatatgcggc gaacgaggac cgagcgaaca
      181 tgtcggttct gcgccggtcc agaaaagggc ccgttgtcgc gcgcagtaag gcggcggcaa
      241 acaccggcgg ccgacgctgt tcatccgcga gcccaagatc cgagaacggc ctgtccataa
      301 ggccatgtcc cgcgggctcg attcgcgcgg cgcaatcgcg ccgtattctt cgacgttcta
      361 tcagacgccg cagaagtgct tgcaatgacc ggcaggctga ggttcgcgca gggcggcgct
      421 cgagcgggag aacagcacgc ggcagcaact cgaacgcttc atcgaggaca ccgtcgaggc
      481 cgcggcagtg cgccactttg ggcaggcatg tgaccgcatc gaggccggcg aacgcggccg
      541 cgacctgcgg gccaggacgg agaggcctac gtcatcgtta ttggcccgcc gacaccacgc
      601 acacctcaca cgcgctgcac cacatgacac gcgaggtcct accagtgagc gacaagctcc
      661 gcgaccacgt cgacgcagcg ttgccgggca tgttctcgct cgcgctcctc gcgtgcgggt
      721 cacgcctgga cgcccaccgc cacgttgccg cgaccctccg ggcgatcgac cacgtcgacg
      781 cgaccgtcag agcccgagcg tcacgagcag ccactcgacg agctcgtcgg cggtgcctcc
      841 gggcgccagc gcgaccgcgc cgatccccca gcgggtccgc cagctcggcg cgaaccactc
      901 ttggatctcc tggtcgggct gcggcgcgag cagtaggccc ccgaggcacg ctccgccgcg
      961 gtcctcgtcg cgcagggcgt cgcggtagat gtgcagcgac tcgaacgcgt cggcgaggtt
     1021 gcggcggccg gcgcggtact tcgcgtcgag cacgacccac cgaccggtac cgccgggtgt
     1081 gtcgagcgtg agcacgaggt cgggacggcg ctcaccgctg agtgaaaagc gggggtggtg
     1141 cccgtggttg aaccagccgt aaaacgtggg gttgaactcg atcccgagcc gcgcacccgc
     1201 cgggctgacg gcgtcgaagc ggatgccctc cccggtcgac gcgaggtcga ggagccgctg
     1261 cgtgttccgg gagctccacg tccagtctgc aaggcatgaa cgcagcgacc gctcgacggc
     1321 gagcaggcac cacagctcgt agagcgagta gctcgggcgc acggccgcgg ctgggtcgga
     1381 tgccgccccg tggagacgga agcgcggcga gcagaagcgg cggccgagcg cgtggaggcg
     1441 ggcgtacgcg atgtcgtcgg cgatcacgag gagcgccgcc tcggtcgcgg gcgtcgggct
     1501 gagtctgcgc aggaagctgc ggtcgcgcag agtcgcgagg cgatcggcgc cggtgcgggc
     1561 acgctcggcc cgggcggcgc accaggctcc ggagtcgtcg gggcgcctgg cccggcgatc
     1621 caggatgtcg gcggcttcgc ggagtcgggc gatggcagtg cgcaggatcc aggcgatata
     1681 acggttggcc gggtggtcga gggttgcatc gaccatcgtc gccggcacca gtggcggcgc
     1741 accgatctgt tccgcggccc gccaggggtg caaccaggcg ccggccgtcg gatggcggcc
     1801 gagccacgcg atcgtcgagc tctcgacgcg ccggatctgc cggagcgggc ggtcctccgg
     1861 ctcgatgcgg tcggcgacgc gagggtgggc gaggaccacg cggagcgcgt cctcgagggc
     1921 cggcaggagc ggcaagaggg cctcggcgaa cagcgtcgcg ctaacgccct cggagccgac
     1981 agcgccctgc ttgccgcccg cgagcccggg cgtgacgcca ggcagccagg cctcgagctc
     2041 ctccagcagc gtcgcccaca gcgcctccga caacttctcg ctgcggggcc gcacgcgcac
     2101 gcgcgcggcg accggtccgt cggcggtctc aagctggagc tgcgtcgcgc cgacccagcg
     2161 gtcgatcggg agtacgaagc agccggggtt cgccgcgagc agcagtcggg cgtccaggcg
     2221 cgcctcgctc gcgccggccg cgagcacctg gtagtgccgg tcctcctcga gcacgaaggt
     2281 cccgtcatcc tcgacgagga cggtgtggcc gtgcagatcg acgattacga tcttcatgac
     2341 cagaacttgg tcagaccggt gctgcgtagg cgagcctgca tctcggcgat cttcgcggcg
     2401 caacgcggca agtaccggga ctgacagtgg tcgaggatca ccgtcagcgc ctgatgtagc
     2461 gccggggtct cctcgccgcg gagtcgcggc aggaccttgc tgaagatcgc ctggtcgagc
     2521 acctcggcga ctgcagccgc atcgagggtg gcggcggcgg cggcatcgag catgccgagg
     2581 atctcgctgg cggtgcggta gccgaagtgg cgacggatcg gggtgagcgc ggcgtggagc
     2641 gcgagcagtt cagtctccgc gacgaggtga cgcgggtgcc ccgcggcggc cctgcgctcg
     2701 aagaacgtgg cgaggtcgac ctgccagaac tcgagcgtga acgcccgatc gagcaccttg
     2761 tcgctgatcg catgggtcgt ctcgtccatg ttgatcgtgc cgccgatgaa caggttgcgg
     2821 ggccatggga cccacggtgg tacgccgttg acgggctcgt cctgcgcgtg gagcaccagc
     2881 ctgtcgccgg tctccatcgc cgacaggaac ggcgcgaagt agtgctcgac gcgggcgagg
     2941 ttcatctcgt cgaggatcag gaagtagggc aggtcgggcc gcttcgccgc ggcgagcacc
     3001 agacgcagcg ccggctcggc ctggtaggtg gcgtcggcct gcaacgcgtt gaagtagccg
     3061 agcagcccgg tcgggtcgcg ccagtccggc gacacgggga ccaccgcgcg atggtgttcg
     3121 acgtcgcggc ccagctgctc gcag
//
LOCUS       DASWNJ010000136         2349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_389765, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000136 DASWNJ010000000
VERSION     DASWNJ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389765"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /gene="trpA"
                     /locus_tag="VGB85_02510"
     CDS             complement(<1..580)
                     /gene="trpA"
                     /locus_tag="VGB85_02510"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEY0132915.1"
                     /translation="MTAAAPTSRIAARFAAVKAEGRAALVGYLTAFDPTRRAAFDRLC
                     AACEAGLAVLELGVPFSDPSADGPTIQAAMVRALAAGATLGGVIDLAAELRRRFDVPI
                     VLFSYGNPLLRLGAEKLVERATQAGVDGLLVVDLPPEHSAPLREPARAAGLDWVGLVA
                     PTTTPRRRASVLGAASGYVYAVILTGVTGAPLD"
     gene            complement(577..1959)
                     /gene="trpB"
                     /locus_tag="VGB85_02515"
     CDS             complement(577..1959)
                     /gene="trpB"
                     /locus_tag="VGB85_02515"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEY0132916.1"
                     /translation="MTDASQASPAPPDSQPLLADFGEFGGRYVAETLMPAVESLARAW
                     PEAWADPGFQADYRRILRDYVGRPSPISEAPRLALACGGGRIFLKREDLNHTGSHKIN
                     NSIGQVLLARRMGKTRIIAETGAGQHGVATATACAYFGLPCVVYMGAEDVARQALNVV
                     RMRLLGAEVVPVSSGSATLKDAINAALRDWVASVETTHYVIGSVMGPDPYPLMVRELQ
                     RVIGEEAREQLKERVGRLPDAVVACVGGGSNAMGIFAAFVPDTEVQLIGVEATGEGVE
                     SGRHAATMAGGQVGVYHGMRSLVLQSEDGQLQEAHSISAGLDYPGVGPEHAHLRATGR
                     ARYMGISDAEALAATALLSRTEGIIPALETALAIAALPRIVSELGPDAIILVSLSGRG
                     DKDMHTVMSHLDPGALELVKDAERGRDAARAAVRGVATPPEAPPPDGEAPRSVVIVDG
                     HELVADKEAT"
     gene            complement(2023..>2349)
                     /gene="rlmJ"
                     /locus_tag="VGB85_02520"
     CDS             complement(2023..>2349)
                     /gene="rlmJ"
                     /locus_tag="VGB85_02520"
                     /inference="COORDINATES: protein motif:HMM:NF016278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEY0132917.1"
                     /translation="NGLKVAHERWPTGCYALWYPIKGPVARGKFHAALMHGTIRKVLC
                     VELYTRPAREDDTLAGSGLILVNPPWRTYAALRGVLPALAALLAEDDAAGDPHLRTRV
                     EWIVGE"
BASE COUNT          302 a          856 c          880 g          311 t
ORIGIN      
        1 ggtcgagcgg ggcccccgtc acgcccgtca ggatgaccgc gtagacgtag ccgctggcgg
       61 ccccgagcac gctcgcgcgg cgccgtggcg tcgtcgtcgg cgcgacgagc ccgacccagt
      121 cgagcccggc ggcgcgcgcc ggctcgcgca gcggggcgct gtgctcgggc ggcaggtcga
      181 cgacgagcag cccgtcgacg ccggcctgcg tcgcgcgctc gaccagcttc tcggccccga
      241 ggcgcagcag cgggttgccg tagctaaaca gcacgatcgg cacgtcgaag cggcggcgca
      301 gctcggccgc gaggtcgatc accccgccga gcgtcgcgcc ggcagcgagc gcgcggacca
      361 tcgccgcctg gatcgtcggg ccgtcggcgc tcggatcgga gaagggcacg ccgagctcga
      421 gcacggcgag gcccgcctcg caggcggcgc acaggcgatc gaaggcggcg cgacgcgtcg
      481 ggtcgaaggc ggtcaggtaa ccgaccagcg ccgcgcgacc ctcggccttg acggcggcga
      541 agcgcgccgc gatccggctc gtcggagccg cggcggtcat gtcgcctcct tgtcggcgac
      601 gagctcgtgg ccgtcgacga tgacgacgct cctgggcgcc tcgccgtccg gtggcggagc
      661 ttcgggcggc gtggcgaccc cgcgcaccgc agcgcgcgcg gcatcgcggc cgcgctcggc
      721 atccttcacc agctcgagcg cgccggggtc gaggtgcgac atcaccgtgt gcatgtcctt
      781 gtcgccgcga cccgacaggc tgaccaggat gatcgcgtcg ggcccgagct cgctgacgat
      841 gcggggcagc gccgcgatcg cgagggccgt ctcgagcgct ggaatgatgc cctcggtgcg
      901 tgacagcagg gcggtggccg cgagcgcctc ggcgtcgctg atgcccatgt agcgggcccg
      961 gccggtcgcg cgcaggtgcg cgtgctcggg gccgacgccg gggtagtcga ggccggcgga
     1021 gatcgagtgc gcctcttgca gctggccgtc ctcgctctgc aacacgaggc tgcgcatgcc
     1081 gtggtacacg ccgacctggc ccccggccat cgtcgccgcg tggcgtccgc tctcgacgcc
     1141 ctcgccggtc gcctcgacgc cgatcagctg gacctccgtg tcgggcacga acgccgcgaa
     1201 gatgcccatc gcgttcgagc caccgccgac gcacgcgacg accgcgtcgg gcaaccgacc
     1261 gacgcgctcc ttgagctgct cccgggcctc ctcgccgatc acgcgttgca gctcgcggac
     1321 catcaagggg taggggtccg ggcccatcac gctgccgatc acgtagtgcg tggtctcgac
     1381 gctggcgacc cagtcgcgca gcgccgcgtt gatcgcgtcc ttgagggtcg ccgagccgct
     1441 gctcaccggc acgacctcgg cgccgagcag gcgcatgcgc acgacgttga gcgcctggcg
     1501 ggcgacgtcc tcggcgccca tgtacacgac gcagggcagg ccgaagtacg cgcacgcggt
     1561 tgccgtggcg acgccgtgct ggcccgcgcc ggtctcggcg atgatccgcg tcttgcccat
     1621 gcgccgggcc agcagcacct gaccgatgct gttgttgatc ttgtgcgagc cggtgtggtt
     1681 gaggtcctcg cgcttgagga agatccggcc gccgccgcac gcgagggcca gccgcggcgc
     1741 ctcgctgatc gggctcgggc ggccgacgta gtcgcgcagg atccggcgat agtcggcctg
     1801 aaagccgggg tcggcccacg cctcgggcca cgcgcgcgcc agcgactcga ccgccggcat
     1861 cagcgtctcg gcgacgtagc gcccgccgaa ttcaccgaag tcggcgagca gcggctggga
     1921 gtccggcggt gctggtgatg cttgcgatgc gtcggtcatg cccgaacgcg aggctaccat
     1981 cgatcgtgcg cgccgagcct gcgcgccggc gcgcgccggt cgtcactcgc cgacgatcca
     2041 ctcgacgcgc gtgcgcagat gcgggtcgcc cgcggcgtcg tcctcggcga gcagcgcggc
     2101 gagggcgggc agcacgccgc gcagcgcggc gtaggtgcgc cacggcggat tgacgaggat
     2161 caggccggag ccggcgaggg tgtcatcctc gcgtgcgggc cgggtgtaca gctcgacgca
     2221 caggaccttg cggatcgtcc cgtgcatcag cgccgcgtgg aacttgcccc gcgcgaccgg
     2281 ccccttgatc gggtaccaca gcgcgtaaca gccggtcggc cagcgctcgt gcgcgacctt
     2341 gaggccgtt
//
LOCUS       DASWNJ010000137         3963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_673737, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000137 DASWNJ010000000
VERSION     DASWNJ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673737"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VGB85_02525"
     CDS             <1..813
                     /locus_tag="VGB85_02525"
                     /inference="COORDINATES: protein motif:HMM:NF013569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-like YCDxxxxGGGW domain-containing
                     protein"
                     /protein_id="HEY0132918.1"
                     /translation="LSTCLAAKCGDSSVQAGVEECDDGNADETDMCTAACKAPSCSDG
                     AQNGAETDVDCGGACGKCGLGDACLANADCGMGLCIADSCAVPKSCKQIKEAAPASKD
                     GNYTIDPDGAGPIGSLVAHCDMDADATCGYTWIRYDDPALLNDQNVYAAKCAAVGMEV
                     IVTRTKPHAQAVYDWNLSQNANLVNVFPKTNGATGINNWQGICQGAPCSFWMTDNANG
                     DVGCSNFEPNGDNNVMWRIYLRQAGCGLQGNWNDANNNVGVQGWVICSPNDC"
     gene            911..2257
                     /locus_tag="VGB85_02530"
     CDS             911..2257
                     /locus_tag="VGB85_02530"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0132919.1"
                     /translation="MPVLPGEVVEGLWRGVLRHAAFRRVWLGAMVSSIGTWMEGLGVQ
                     WLMAEQTGSTVMLGWLAAAQLGPMLVLGMLGGLATDRLDRRKLLLATQTLMMVIALGL
                     AAASAADLATPPVLLALGLLNGVAMAFNTPAWQVLTPRLVPRKELHRAIALQGMQFNL
                     ARVVGPALAGVLLAQTSATVLFALNAVSFVAVLAAIASTPPAPAPAGPPPAVRAQVLE
                     AVRFVFGGKGPRALFTMLVLLSALAAPLQRMLPLFVSDVFHGGEADYGLLLGMFGAGA
                     VLGGLTLGRWPAWYPRHHLIPLALTLTGAFMTLFCATDNLALAGVWIFLAGLVWIWAF
                     SSMMTAVQLLVTDAMRGRVLSVTNMAMFGAMPVGSLLAAVLGDLLGARVGPTHGAAAV
                     QLGVGSMALLLMLLGALLLVFRTPEIDEPTPGADVPQRARSLWRGLTAAGHRPRIA"
     gene            2384..2737
                     /locus_tag="VGB85_02535"
     CDS             2384..2737
                     /locus_tag="VGB85_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0132920.1"
                     /translation="MERDKHVRALLREFLDPAVYTLDFFDDGVSALTAVRTKPPDLVI
                     LEILVPKLDGLGLCRAIKSSTRQPTTPVLVLSILSASDRAKLAGADAFMLKPIERSRL
                     LGTVEKLLASPGEPS"
     gene            2734..>3963
                     /locus_tag="VGB85_02540"
     CDS             2734..>3963
                     /locus_tag="VGB85_02540"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEY0132921.1"
                     /translation="MTINDDAPAVTFARISSGNPEVDHILDGGFPANSINIVMGQPGT
                     GKTIFVQQMVFHNAADDRPVLYLTTLSEPLSKVVKYLQRFAFYDELKVGSAVIYQDIA
                     PELAEHGITALEETLREAIRTHAPKIIVIDSFKALHDLSGSPAEMRRVLYEISGMLAA
                     YETTVFLVGEYSEKQARIQPEFAVADGIIQFFRQERSNRDERFMRVLKLRGSGYAEGV
                     HGFRITPSGLHVFPRLVSPEVPPNYELLAERVTSGVPGLDDLLRGGLWRGSTTLLTGP
                     TGGGKTTIGAQFVLEGLRRGESSLYINFQENPTQLARLFRSLDVDMREAQARGLHVLY
                     ESPVELQIDSVINRVFRMLDEHKLQRVVVDSLGDLATAAGDPQRLHDYMYALMQHFVI
                     HRTTCIVTAEATIGDASS"
BASE COUNT          586 a         1418 c         1337 g          622 t
ORIGIN      
        1 ctgagcacct gcctcgcggc caagtgcggc gacagctcgg tgcaggccgg cgtcgaggag
       61 tgcgacgacg gcaacgccga cgagaccgac atgtgcacgg cggcatgcaa ggcgccgagc
      121 tgctcggacg gcgcgcagaa cggcgcggag acggacgtcg actgcggcgg cgcctgcggc
      181 aagtgcggcc tgggtgacgc ctgcctcgcc aacgccgact gcggcatggg cctgtgcatc
      241 gccgacagct gcgcggtgcc caagagctgc aagcagatca aagaggccgc gccggcgagc
      301 aaggacggca actacacgat cgaccccgac ggcgcgggcc cgatcggctc gctggtcgcc
      361 cactgcgaca tggacgccga cgcgacctgc ggctacacgt ggatccgcta cgacgacccc
      421 gcgctgctca acgatcagaa cgtgtacgcc gccaagtgcg cggcggtcgg catggaggtg
      481 atcgtcacgc ggaccaagcc gcacgcccag gccgtctacg actggaacct ctcgcaaaac
      541 gccaacctcg tcaacgtgtt cccgaagacc aacggcgcga ccgggatcaa caactggcag
      601 ggcatttgcc agggcgcgcc ctgcagcttc tggatgaccg acaacgccaa cggcgacgtc
      661 ggctgctcga acttcgagcc caacggcgac aacaacgtga tgtggcggat ctacctgcgc
      721 caggccggct gcggcctgca gggcaactgg aacgacgcga acaacaacgt cggggtccag
      781 ggctgggtga tctgctcgcc caacgactgc tgagatccac tgctgagatc cacgctggcc
      841 cgctcggcgg gtcggtctat actgcggcat cccgcagcac ctgcaggtcg atcccgccgc
      901 gtccgccgtc ttgccggtcc tccccggcga ggtggtggag gggctgtggc gcggcgtgct
      961 gcgccacgcc gcgtttcgcc gggtctggct cggcgcgatg gtctcgtcga tcggcacgtg
     1021 gatggagggc ctcggcgtgc agtggctcat ggccgagcag accggctcga ccgtgatgct
     1081 cgggtggctc gccgccgccc agctcggccc catgctcgtg ctgggaatgc tcggcggcct
     1141 cgccaccgac cgcctcgacc gccgcaagct gctgctcgcc acccagacgc tcatgatggt
     1201 gatcgcgctc gggctcgcgg ccgccagcgc cgccgacctg gcgacgccgc cggtgctgct
     1261 ggcgctgggc ctgctcaacg gcgtggcgat ggccttcaac acgcccgcct ggcaggtgct
     1321 gacgccgcgc ctggtccccc ggaaggagct gcaccgcgcg atcgcgctgc agggcatgca
     1381 gttcaacctg gcgcgcgtcg tcggcccggc gctcgccggc gtgctgctgg cccagaccag
     1441 cgcgaccgtg ctgttcgccc tcaacgcggt cagcttcgtc gccgtgctcg cggcgatcgc
     1501 cagcaccccg cccgcgcccg cgcccgcggg cccgcccccg gccgtgcgcg cgcaggtgct
     1561 cgaggccgtc cgcttcgtgt tcggcggcaa gggtccgcgc gcgctgttca ccatgctcgt
     1621 gctgctgagc gccctcgcgg cgccgctgca gcgcatgctg ccgctgttcg tctcggacgt
     1681 gttccacggc ggcgaggccg actacggcct gctgctcggg atgttcggcg ccggtgcggt
     1741 gctcggcggc ctgacgctcg ggcgctggcc ggcctggtac ccgcgccacc acctgatccc
     1801 gctggcgctg acgctgaccg gcgccttcat gaccctgttt tgcgccaccg acaacctcgc
     1861 gctcgccggc gtgtggatct tcctcgctgg acttgtttgg atctgggcgt ttagcagcat
     1921 gatgaccgcg gtgcagctgc tggtgaccga cgcgatgcgc ggacgcgtgc tgtcggtcac
     1981 caacatggcg atgttcgggg cgatgccggt cggcagcctg ctcgccgccg tgctcggcga
     2041 tctgctcggg gcccgcgtcg gcccgaccca cggagccgcc gcggtccagc tcggcgtcgg
     2101 ctcgatggca ttgctgctga tgctgctcgg cgcgttactt ctggtgtttc gtacgccgga
     2161 gatcgacgag ccgacgcccg gtgcggacgt cccgcagcgc gcgaggagcc tgtggcgcgg
     2221 gctgactgcc gcaggtcatc gcccgcgtat cgcatgagcg ggaaacccgc tgtgaggccg
     2281 acaggagccc gaatactggc attcgcgttc gccgcgcgtt tatcgcattt gcccggccgg
     2341 cgaatcggcg catccctggg tcgctgtgca ctcggtattc gcggtggagc gggacaagca
     2401 cgtaagggcg ctcctgcggg agttcctcga ccctgccgtg tatacgctcg acttcttcga
     2461 cgatggtgtc tcggccctca cggcggtgcg aacaaagcca ccggacctcg tgatcctcga
     2521 gatcctcgtg cccaagctcg acggtctcgg gctgtgccgg gcgatcaagt cgagcacgcg
     2581 gcagccgacc acgccggtgc tggtgctgag catcctgagc gcgagtgacc gcgccaagct
     2641 cgccggcgcc gatgcgttca tgctcaagcc gatcgaacgc agtcgcctgc ttgggaccgt
     2701 cgagaagctg ctcgccagcc cgggtgaacc atcatgacca tcaacgacga cgccccggcg
     2761 gtgaccttcg cgcggatctc ctccggtaac ccggaggtcg accacatcct cgacggcggc
     2821 ttcccggcca actcgatcaa catcgtcatg ggccagcccg gcaccggcaa gacgatcttc
     2881 gtccagcaga tggtgtttca caacgccgcc gacgaccgcc cggtcctgta cctgacgacg
     2941 ctgtccgagc cgctgtccaa ggtggtcaag tacctccagc gcttcgcttt ctacgacgag
     3001 ctcaaggtcg gcagcgcggt catttatcag gacatcgcgc ccgagctggc cgagcacggc
     3061 atcaccgcgc tcgaggagac gctgcgcgag gcgatccgca cccacgcgcc gaagatcatc
     3121 gtcatcgact cgttcaaggc gctgcacgac ctgtccgggt cgcccgcgga gatgcgccgc
     3181 gtgctctacg agatctccgg gatgctcgcg gcctacgaga ccaccgtgtt cctggtcggc
     3241 gagtacagcg agaagcaggc gcggatccag cccgagttcg cggtcgccga cggcatcatc
     3301 cagttcttcc gccaggagcg cagcaaccgc gacgagcgct tcatgcgggt gctcaagctg
     3361 cgcggcagcg gctacgccga gggcgtgcac gggtttcgca tcacgccgtc gggcctgcac
     3421 gtgttccccc ggctcgtcag ccccgaggtc ccgccgaact acgagctgct cgcggagcgc
     3481 gtcacctccg gggtgcccgg cctcgacgac ctgctccgcg ggggcctgtg gcgcggcagc
     3541 acgaccctgc tgaccggccc gaccggcggc ggcaagacga cgatcggcgc gcagttcgtg
     3601 ctcgagggcc tgcgccgcgg tgagtcgagc ctctacatca atttccagga gaacccgacg
     3661 cagctggccc gcctgttccg ctcgctcgac gtcgacatgc gcgaggccca ggctcgcggc
     3721 ctgcacgtgc tctacgagtc gccggtcgag ctccagatcg acagcgtgat caaccgcgtg
     3781 tttcgcatgc tcgacgagca caagctgcag cgggtggtcg tcgactcgct cggcgacctg
     3841 gcgaccgcgg ccggcgatcc gcagcggctc cacgattata tgtatgcgct gatgcagcac
     3901 ttcgtcattc accggacgac ctgcatcgtc accgccgagg cgacgatcgg cgacgcctcg
     3961 agc
//
LOCUS       DASWNJ010000138         1694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_532253, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000138 DASWNJ010000000
VERSION     DASWNJ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532253"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VGB85_02545"
     CDS             <1..402
                     /locus_tag="VGB85_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132922.1"
                     /translation="PRSIDTSHHPGPRAPTAVKTAGAVALTVGLVGLATMAGGLVRKS
                     QAQKAAGPLDATYKQQGDGFTADQRSEWNALAATGTAGQRLAVAGGVCGAVLLVAGAA
                     LVARGHVLASRPRVSVMAAPDRAGLWVEGRF"
     gene            complement(422..793)
                     /locus_tag="VGB85_02550"
     CDS             complement(422..793)
                     /locus_tag="VGB85_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132923.1"
                     /translation="MIATGAACNSFAGTRSTPTIFGGAAGGPSDVPVADVPSIDVLAV
                     ESGAVVASVVVTMPSSAPDCEALPEVVGLAAPLLLGSACVAAHPVPEPLCSSQTWPSW
                     GSPSTPSPHAGMVRTSKKTRV"
     gene            822..>1694
                     /locus_tag="VGB85_02555"
     CDS             822..>1694
                     /locus_tag="VGB85_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132924.1"
                     /translation="MMALDGAAPVSLAAVGGNFFKGAIEVQGAAMNGPHTVLITALGA
                     GRADTWPASFEVDAPDPGENIWVRTSDVPNSQTRAIAVDPESNVYDVGTVGTGAAARL
                     AVSKRDEFGTLAWPGAWATYNDERSWGEDIAVGPDGLIYVLGNYEDAAKHVRWWLLTL
                     DPGVGIVVDEQVGEIDQPAHGMSISPKGDLAIVTTVTVWNVQLQKDDPQTQVIIRPKD
                     LLGVIVPWGYSPPALDWLFTEVPEDVLIDGERVFVTGSVEGKQPDIKNDDMIRKRVFV
                     IEVNFQGEVVREYVA"
BASE COUNT          285 a          557 c          611 g          241 t
ORIGIN      
        1 ccccggtcca tcgataccag tcatcacccc ggaccacgag caccgaccgc cgtcaagacg
       61 gccggcgccg tcgcgctgac cgtcggtctc gtgggcttag cgacgatggc cgggggcctc
      121 gtgcgcaaaa gccaggcgca gaaggccgcg ggtccgctgg acgccacgta caagcaacaa
      181 ggcgacggct tcacggccga ccagcgtagc gaatggaatg cgctggcggc gaccgggacg
      241 gcgggacagc gcctggccgt cgcgggcggc gtgtgcggcg cggtgctgct ggtggccggc
      301 gcggccctcg tcgcgcgtgg ccacgtgctg gcgtcacgtc cccgggtgtc ggtcatggcg
      361 gcccccgacc gcgccgggct atgggtggag gggcgatttt gatatagagg ggtatgcggc
      421 gtcacacgcg ggtctttttg ctggttctga ccatgccggc gtgtggactc ggcgtgctgg
      481 gcgagcccca cgagggccag gtctgcgacg agcacagcgg ctccggtacc ggatgcgccg
      541 cgacgcaggc cgatccgagc agcagcggcg cggcgagccc gacgacctcg ggcagcgcct
      601 cgcagtccgg cgccgacgag ggcatcgtca cgaccacgga ggccacgacg gcgcccgact
      661 ccaccgcgag cacgtcgatg gacggcacgt cggcgaccgg tacgtccgag gggccgccgg
      721 cggccccgcc gaagatcgtc ggggtcgacc ttgtcccggc gaagctgttg caggcggcgc
      781 cagtggcgat cacagtcgag gccctcgacg ccaccgaggt catgatggcg ctggacggcg
      841 cggcaccggt ctccctggct gccgtcggag ggaatttttt caagggtgcg atcgaggtcc
      901 agggcgccgc gatgaacgga ccccacaccg tactgatcac ggccctcggc gccggccgag
      961 ccgacacgtg gcccgcgagc ttcgaggtcg acgccccgga tcccggcgag aacatctggg
     1021 tcaggaccag tgacgtgccg aacagccaga cccgggcgat cgcggtcgac cccgagagca
     1081 acgtctatga tgtgggcacc gtgggcaccg gcgcggccgc tcgcctggcc gtcagcaagc
     1141 gcgacgaatt cggcaccctg gcgtggcccg gcgcgtgggc cacctacaac gatgaacggt
     1201 cgtggggcga ggacatcgcc gtcggccccg atggcctgat ctacgtgctc gggaactacg
     1261 aggacgcggc caagcacgtg cgctggtggc tgctcacgct cgaccccggc gtcgggatcg
     1321 tcgtcgacga gcaagtcgga gagattgacc agccggcaca tggaatgagc atctcgccga
     1381 agggagatct cgccatcgtg acaacagtca cggtctggaa cgtgcagcta caaaaagatg
     1441 acccgcagac ccaggtgatc atccgcccga aggatctgct cggcgtgatc gtgccgtggg
     1501 gttactcgcc ccccgccctg gattggctct ttaccgaggt ccccgaagac gtcctgatcg
     1561 acggcgagcg ggtcttcgtg accggaagcg tcgagggcaa gcaaccggac atcaagaacg
     1621 atgacatgat ccgcaagcgt gtgttcgtga tcgaggtgaa ttttcagggc gaggtcgtcc
     1681 gcgagtatgt cgcg
//
LOCUS       DASWNJ010000139         7218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_638978, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000139 DASWNJ010000000
VERSION     DASWNJ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7218
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638978"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VGB85_02560"
     CDS             <1..645
                     /locus_tag="VGB85_02560"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0132925.1"
                     /translation="HHMEEVQAQLRHAQKAVSLLGKSSGGTVIVTSQIEGTVLRRYAT
                     PGAQVTPNDEPLVEIGNPKDLWVVAEVFQDDLQFMRAGSKVTLEFASLKQGVPGHIEA
                     LGVLVDTGLRRAPVYVKIDDENVNLTPGMFARANIQAPAEEGVTVPKGAVLIKDGSST
                     VVYVEQGDSMFVRRDVLLGYTFGEYVQILSGLKPGERVAVDGALLLDAAAQLLL"
     gene            664..3804
                     /locus_tag="VGB85_02565"
     CDS             664..3804
                     /locus_tag="VGB85_02565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEY0132926.1"
                     /translation="MIRALIRACIQKRFVAVVVTLLVAAYGLSAYLKTPIEAYPDVTN
                     VQVNVVAQLSGLAPEEVERQITIPLERALNGTPKMIQMRSESLFGLSIIWCVFEDGAD
                     AFQARTRVAERLTGVEIPESAEVRVAPDATPLGKVYYYRLVSDRHSLYDMRSEQEWTV
                     ARILKQVPGVADVVGMGGYLKELHVEVDPARMAAYGLTLADISEALEKSNKNVGGGFQ
                     AHGDQQLVIRGIGYLRTAKDVEDVVIEAEDGTPVTVGDVTRVIQAYTPRQGTVGYNDK
                     RDIVEGIVLLRRGENPDTVLQALHTKIDELNAKVLPEGMTIEPMYDRSVLIAYTLDTV
                     NHNLLHGFLLIIAIVWLFLRSMRGSLIVASIIPLSLLSAFAGLYLLGLPANLISMGAI
                     DFGILVDGAVVLVENVYHQAHLVRPKTRREMLGLVARSAIDVSRPTFYAMAIIIAALI
                     PVFALESVEGRIFRPLALTYSFALIGALVFSLTVVPALCAIFMRPKDADIKEPRSLEY
                     LRRGYRKVLGLLLRVRLVPAALGLALLLGAGAVGSQVGTEFLPELDEGDLYIFVEMPP
                     SISLTSGQDVLAEVRRRIMEFPEVTSTPSEQGRPEDGLDNEGTNMAKVFARLKPKAEW
                     RENLSKEQLIDDIRASLSKIPGVRFNFSQPIKDSVEEAVSGVRGKIVVKVFGSDLLEM
                     RSTLMNVIDTIEDVPGVVDLGLYRDSSVPQLQINFDRSALARSGISVDTASTVIETAM
                     AGRIVTNMWQGERLVPVRVRLPVIEKADVDRIKDLLIPSAAGGRIPLHELADISIATG
                     RASVIREANSRYLALKFNVEGRDMGSTVDEAIKVVAEKVKVPEGQFLVWGGEFENQQR
                     AMARLSFIVPIALLIVLALLYGALGSGTSALAVILCTPFALSGGIFALAATGIPLSVS
                     AAVGFIALLGQVSLAGLLIVSAVDNLRLEGVELRTAVIEGAVTRLRALLMTALLAMSG
                     LMPMAISTGLGSETQKPFAVVIIGGMATTLFVALFVLPAIYTLVTPRRLAGPQDPEDE
                     LPSMTSGVIKPPEHAI"
     gene            3820..5184
                     /locus_tag="VGB85_02570"
     CDS             3820..5184
                     /locus_tag="VGB85_02570"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY0132927.1"
                     /translation="MNSFPAKLLTLVVATGLCAPSPAWAAPSPFGPQPQPGTNPNDAV
                     PPQRAADPPPVALEAIEVKDSVTLAEVFDILKRRSPRYKVVQYEVDIAKSESRAARVL
                     PNPVLNLAILYLNQGFNQNGVATYYANVTLPLLIAGQRRMRVKTADVGIKAAEANVRS
                     NFHDLAHEARARFVELQAAQERVKVLDEALVDLGRVQNVIVERARSGVETQYDTVRIA
                     IETSAWKARRAEEEAEAQDIAGRLGILLGVPSWYPRAEGELKELGVHGDPDQMWPEIE
                     RTQPGIEAARREEAYAVKNIDLAKRERWPVPSVTVGTVAIQNYYSISTTVGLTVPIPV
                     FDWGQGLLARANARSAQSRLQKDTVIAETQAELKRAMRLLEHRRKAVATFEQEVFSKI
                     PTLQQMAEDSFRSGQTQLIELLDATRARFEVKMTHIDMREAVIQAEIDVLAVTGRIEE
                     IRPR"
     gene            5181..5879
                     /locus_tag="VGB85_02575"
     CDS             5181..5879
                     /locus_tag="VGB85_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132928.1"
                     /translation="MTATRPTRHLASVAVAVLALPVVMGPGRCGFALDFTETILITDE
                     INRVTLGVDDGSIVATMYDREATLLKRHTFGFEPSLGDVSRTVEDGVLKLEARCKYEG
                     NCRFDHMFELPLGIAFEVDMLDSQISLGYIDSDIDINFTTGWFKGVRLAAPNFTLSLG
                     VGDVTVDFAAAPEAVTIALEEGDVVLEVPAGLYRCALSASGAVTNNGVVCDDAAPAVL
                     DVQVQTGNITVTGT"
     gene            5876..6562
                     /locus_tag="VGB85_02580"
     CDS             5876..6562
                     /locus_tag="VGB85_02580"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEY0132929.1"
                     /translation="MSRSRLAARVLPLGLLLASACGVVIYDTSSRVLILEPVERMVFD
                     SDSGAVEVFAFNRTAISLFYYLTGFETGIADVGHELDGEALRAVIACEGDDLCQADFY
                     AEVPLGTAIEIRAASGDVKLTGVDAEVTAVVTAGAVEGVGLDSPTFSLEVGTGAVTLA
                     WDSPPMTLNITVAAGDVSLTLPPGSYQCDFTTADGAVESQGITCDPAAAATLKISVDS
                     GDIHLQGTAP"
     gene            6559..>7218
                     /locus_tag="VGB85_02585"
     CDS             6559..>7218
                     /locus_tag="VGB85_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132930.1"
                     /translation="MNYSNHHGRMYARLAALALPLALVGGPAHAAPDLDFLASAGLGD
                     DLGGRPVAIDLTNDGVVLASVREGQGTLVRLDRSGESLANTRTLNTGVDHLAVDRGSG
                     NVTVVGPDGVAVFDAELAPLWQRPLTTRAGERRVAVGERGTVAAIAGGTLHIFAADGT
                     LLGQAEPGDDFTRGLAVLDDAGIVVTTGWSRRTACDSAVDVAALTAFAHDGAPRWRAY
                     GD"
BASE COUNT         1127 a         2734 c         2287 g         1070 t
ORIGIN      
        1 caccacatgg aggaggtcca ggcccagctg cgccacgcgc aaaaggccgt ctccttgctc
       61 ggcaagagct ccggcggcac cgtcatcgtc acctcgcaga tcgaaggcac cgtgctgcgc
      121 cgctatgcga cgcccggcgc ccaggtcacc cccaacgacg agccgctcgt cgagatcggc
      181 aaccccaagg acctgtgggt cgtcgccgag gtgttccagg acgacctcca gttcatgcgc
      241 gccggctcca aggtcaccct cgagttcgcc tcgctcaagc aaggcgtccc cggccacatc
      301 gaggcgctcg gcgtgctcgt cgacaccggc ctgcgccgcg ccccggtcta cgtcaagatc
      361 gacgacgaga acgtcaacct gacgcccggc atgttcgccc gcgccaacat ccaggcgccc
      421 gcggaggagg gcgtcacggt gcccaagggc gccgtgctga tcaaggacgg cagcagcacc
      481 gtcgtctacg tcgagcaggg tgacagcatg ttcgtgcgcc gcgacgtgct gctcggctac
      541 accttcggcg agtacgtgca gatcctgtcg ggcctcaagc ccggcgagcg cgtcgccgtc
      601 gacggcgccc tgctgctgga cgctgcggcc cagcttctcc tctgacctgg accggagcct
      661 acaatgatcc gcgcactgat tcgcgcttgc atccaaaagc gcttcgtcgc cgtcgtcgtc
      721 accctgctgg tggccgcgta cggcctgtcc gcctacctca agacgccgat cgaggcctac
      781 ccggacgtca ccaacgtcca ggtcaacgtc gtcgcccagc tgtccggcct cgcccccgag
      841 gaggtcgagc gccagatcac gatcccgctc gagcgcgccc tcaacggcac gccgaagatg
      901 atccagatgc gcagcgagag cctgttcggg ctctcgatca tctggtgcgt cttcgaggac
      961 ggcgccgacg ccttccaggc gcgcacacgc gtcgccgagc gcctgaccgg cgtcgagatc
     1021 cccgagtccg ccgaggtccg cgtcgccccc gacgccaccc cgctcggcaa ggtctactac
     1081 taccgcctcg tcagcgaccg ccactcgctc tacgacatgc gctccgagca ggagtggacc
     1141 gtcgcgcgca tcctcaagca ggtgcccggc gtcgccgacg tcgtcggtat gggcggctac
     1201 ctcaaggaac tgcacgtcga ggtcgacccc gcgcgcatgg ccgcctacgg cctgacgctg
     1261 gccgacatca gcgaggccct ggaaaaatcg aacaagaacg tcggcggcgg cttccaggcc
     1321 cacggtgacc agcagctggt catccgcggc atcggttacc tgcgcaccgc caaggacgtc
     1381 gaggacgtag tgatcgaggc cgaggacggc acgccggtga ccgtcggcga cgtcacccgc
     1441 gtgatccagg cgtacacccc gcgtcagggc accgtcggtt acaacgacaa gcgcgacatc
     1501 gtcgagggca tcgtcctgct gcgccgcggc gagaaccccg acacggtgct gcaggccctg
     1561 cacaccaaga tcgacgagct caacgccaag gtcctgcccg agggcatgac gatcgagccg
     1621 atgtacgacc gcagcgtgct gatcgcgtac acgctcgaca cggtcaacca caacctgctg
     1681 cacggctttt tgctgatcat cgcgatcgtg tggctgttcc tgcgcagcat gcgtggctcg
     1741 ctgatcgtcg cctcgatcat cccgctctcg ctgctgagcg cgttcgccgg cctgtacctg
     1801 ctcggcctgc cggccaacct gatctcgatg ggcgcgatcg acttcggcat cctcgtcgac
     1861 ggcgccgtcg tgctcgtcga gaacgtctat caccaggccc acctcgtccg gccgaagacg
     1921 cggcgcgaga tgctcgggct ggtcgcccgc tcggcgatcg acgtgtcgcg cccgaccttt
     1981 tatgcgatgg cgatcatcat cgccgcgctg atccccgtgt tcgcgctcga gagcgtcgag
     2041 ggccgcatct tccgcccgct cgcgctgacc tacagcttcg cgctcatcgg cgcgctggtg
     2101 ttctcgctga cggtcgtgcc ggcattgtgc gcgatcttca tgcggcccaa ggacgccgac
     2161 atcaaggagc cgcgcagcct cgagtacctg cgccgcggct atcgcaaggt cctgggcctg
     2221 ctgctgcgcg tccgtctggt cccggccgcc ctcggcctcg cgctgctgct cggcgccggc
     2281 gccgtcggct cgcaggtcgg caccgagttc ctgcccgagc tcgacgaggg cgacctctac
     2341 atcttcgtcg agatgccgcc gagcatctcg ctgacgagcg gccaggacgt gctcgccgag
     2401 gtccggcgcc ggatcatgga gttcccggag gtcacgagca ctccctcgga gcaggggcgc
     2461 ccggaggacg gcctcgacaa cgagggcacc aacatggcca aggtgttcgc ccgcctcaag
     2521 cccaaggccg agtggcggga gaacctcagc aaggagcagc tgatcgacga catccgcgcc
     2581 tcgctgagca agatccccgg cgtgcgcttc aacttctcgc agccgatcaa ggacagcgtc
     2641 gaggaggccg tcagcggggt gcgcggcaag atcgtcgtca aggtgttcgg ctccgacctg
     2701 ctcgagatgc gcagcaccct gatgaacgtc atcgacacga tcgaggacgt gcccggcgtg
     2761 gtcgacctcg gcctctaccg cgactcgagc gtgccgcagc tgcagatcaa cttcgaccgc
     2821 tcggccctgg cccgcagcgg gatcagcgtc gacaccgcga gcaccgtcat cgagaccgcg
     2881 atggccggcc gcatcgtcac caacatgtgg cagggcgagc gcctcgtgcc cgtgcgcgtg
     2941 cgcctgccgg tgatcgagaa ggccgacgtc gaccgcatca aggacctcct gatcccctcg
     3001 gccgcgggcg gccgcatccc gctgcacgag ctcgccgaca tctcgatcgc caccggtcgc
     3061 gcctccgtca tccgcgaggc caacagccgc tacctcgccc tcaagttcaa cgtcgagggc
     3121 cgcgacatgg gctcgaccgt cgacgaggcg atcaaggtcg tcgccgagaa ggtcaaggtc
     3181 cccgagggcc agttcctggt gtggggcggc gagttcgaga accaacagcg cgcgatggct
     3241 cgcctctcgt tcatcgtacc gatcgccctg ctgatcgtgc tcgccctgct ctacggggcg
     3301 ctcggctccg ggaccagcgc gctcgccgtc atcctgtgca cgcccttcgc gctcagcggc
     3361 ggcatcttcg cgctggccgc caccggcatc ccgctgtcgg tcagcgccgc ggtcggcttc
     3421 atcgccctgc tcggccaggt ctcgctcgcc ggcctgttga tcgtcagcgc cgtcgacaac
     3481 ctgcgcctcg agggcgtcga gctgcgcacc gcggtgatcg agggcgcggt cacccgactg
     3541 cgcgccctgc tgatgaccgc cctgctcgcg atgtcgggcc tcatgccgat ggcgatctcc
     3601 accggcctcg gcagcgagac gcagaagccc ttcgccgtcg tgatcatcgg cggcatggcg
     3661 acgaccctgt tcgtcgccct gttcgtcctg cccgccatct acacgctcgt gaccccgcgc
     3721 cgtttggccg gtccgcaaga ccccgaggac gagctaccct ccatgacctc gggcgtcatc
     3781 aagccccccg aacacgccat ctgacgacgg acgcaccccg tgaactcctt tccagcgaag
     3841 ctcctgacgc tcgtcgtcgc caccggcctc tgcgcgccgt ccccggcgtg ggccgccccg
     3901 tcgccgttcg ggccccagcc gcagcccggc accaacccga acgacgcggt cccgccgcag
     3961 cgcgcggccg acccaccgcc cgtcgccctc gaggcgatcg aggtcaagga cagcgtcact
     4021 ttggccgagg tcttcgacat cctcaagcgc cgcagcccgc gctacaaggt cgtccagtac
     4081 gaggtcgaca tcgccaagtc cgagagccgg gcggcccgcg tactgcccaa cccggtgctc
     4141 aacctggcga tcttgtacct caaccagggg tttaaccaga acggcgtggc gacctactac
     4201 gccaacgtca ccttgccgct gctgatcgcc ggacagcgcc gcatgcgggt caagaccgcc
     4261 gacgtcggca tcaaggcggc cgaggccaac gtccgctcca actttcacga cctcgcccac
     4321 gaggcgcgcg cccgcttcgt ggagcttcag gccgcgcagg agcgggtcaa agtgctcgac
     4381 gaggcgctcg tcgacctcgg gcgcgtgcaa aacgtcatcg tcgagcgcgc gcgctccggc
     4441 gtcgagaccc agtacgacac cgtgcgcatc gccatcgaga cctcggcctg gaaggcgcgc
     4501 cgcgccgagg aggaggccga ggcccaggac atcgccgggc gactcggcat cctgctcggc
     4561 gtgccctcgt ggtacccgcg cgcggagggc gagctcaagg agctcggcgt ccacggcgac
     4621 cccgaccaga tgtggccgga gatcgagcgg actcagcccg gcatcgaggc ggcgcggcgc
     4681 gaggaggcct acgccgtcaa gaacatcgac ctcgccaagc gcgagcgctg gccggtcccc
     4741 tcggtcaccg tcggtacggt cgcgatccag aactactact cgatctcgac caccgtcggc
     4801 ctgactgtcc cgatccccgt gttcgactgg ggtcagggcc tgctcgcgcg agccaacgcc
     4861 cgcagcgcgc agtcccgcct gcaaaaagac accgtcatcg ccgagaccca ggccgagctc
     4921 aagcgcgcga tgcgactgct cgagcaccgg cgcaaggccg tcgccacctt cgagcaggag
     4981 gtcttctcga agatccccac cctgcaacag atggccgagg acagctttcg cagcggccag
     5041 acccagctga tcgaattgct cgacgccacg cgcgcccgct tcgaggtcaa gatgacccac
     5101 atcgacatgc gcgaggccgt catccaggcc gagatcgacg tgctcgccgt gaccggtcgc
     5161 atcgaggaga tccgcccgcg atgaccgcca cgcgacccac ccgccacctc gcctccgtcg
     5221 ccgtcgccgt gctcgcgctg cccgtcgtca tgggcccggg ccgctgcggc ttcgccctcg
     5281 acttcaccga gacgatcctg atcaccgacg agatcaatcg cgtcacgctc ggcgtcgacg
     5341 acggcagcat cgtcgccacc atgtacgacc gcgaggcgac cctgctcaag cgccacacct
     5401 tcggcttcga gccgagcctc ggcgacgtct cgcggaccgt cgaggacggc gtgctcaagc
     5461 tcgaggcgcg ctgcaagtac gagggcaact gccgcttcga ccacatgttc gagctcccgc
     5521 tcggcatcgc cttcgaggtc gacatgctcg actcgcagat cagcctcggc tacatcgaca
     5581 gcgacatcga catcaacttc accacgggct ggttcaaggg cgtgcgcctc gcggcgccga
     5641 acttcaccct gtccctcggg gtcggcgacg tcaccgtcga cttcgcggcc gcccccgagg
     5701 cggtgacgat cgccctcgag gagggggatg tcgtgctcga ggtgccggca ggcttgtacc
     5761 gctgcgcgct ctcggccagc ggcgccgtca ccaacaacgg cgtcgtctgc gacgacgccg
     5821 cgcccgccgt gctcgacgta caggtccaga ccggcaacat cacggtgacc gggacatgag
     5881 caggtcacgc ctcgccgccc gggtgctccc gctgggcctc ctgctggcct cggcctgcgg
     5941 cgtcgtcatc tacgacacca gctcgcgcgt gctgatcctc gagccggtcg agcgcatggt
     6001 gttcgactcg gacagcggcg ccgtcgaggt cttcgcgttc aaccgcaccg cgatcagcct
     6061 gttctactac ctcaccggct tcgagaccgg catcgccgac gtcggccacg agctcgatgg
     6121 tgaagcattg cgagcggtca tcgcctgcga gggcgacgac ctgtgccagg ctgacttcta
     6181 cgccgaggtg ccgctcggca ccgcgatcga gatccgcgcc gccagcggtg acgtcaagct
     6241 gaccggagtc gacgccgagg tcaccgcggt cgtcaccgcg ggcgccgtcg agggcgtcgg
     6301 cctcgacagc cccacgttta gcctcgaggt cgggaccggc gccgtcacgc tggcgtggga
     6361 cagcccaccg atgacgctca acatcaccgt cgccgccggc gacgtctcgc tgacgctgcc
     6421 gcccggcagt tatcagtgcg acttcacgac ggccgacggc gcggtcgaga gccagggcat
     6481 cacctgcgac ccggcggccg ccgccacgct caagatctcc gtcgacagcg gggacatcca
     6541 cctccagggg acagcgccat gaactactcg aaccaccacg gccgcatgta cgcccgtctc
     6601 gctgcgctcg cgctgccgct cgccctcgtc ggcggcccgg cccacgcggc acccgacctc
     6661 gacttcctcg cgtccgccgg cctcggcgac gacctcggcg gccgccccgt ggcgatcgac
     6721 ctgacgaacg atggcgtcgt gctggccagc gtgcgcgaag ggcagggcac cctcgtccgc
     6781 ctcgaccgct ccggtgagtc gctcgcgaac acccgcaccc tcaacaccgg cgtcgaccac
     6841 ctcgcggtcg accgcggctc gggcaatgtc accgtcgtcg gcccggacgg cgtcgccgtg
     6901 ttcgacgccg agctcgcgcc gctgtggcag cgcccgctga ccacccgcgc gggcgagcga
     6961 cgcgtcgccg tcggcgaacg gggcaccgtc gccgcgatag cgggcggcac cctgcacatc
     7021 ttcgcggccg acgggaccct gctcggccag gccgagcccg gtgacgactt cacgcgcggc
     7081 ctcgcggtcc tcgacgacgc cgggatcgtc gtgacgaccg gctggagccg ccgcaccgcc
     7141 tgtgacagcg cggtcgacgt cgccgcgttg acggccttcg cccacgacgg cgccccgcgc
     7201 tggcgggcct acggcgac
//
LOCUS       DASWNJ010000140         3759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_355757, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000140 DASWNJ010000000
VERSION     DASWNJ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355757"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VGB85_02590"
     CDS             <1..209
                     /locus_tag="VGB85_02590"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEY0132931.1"
                     /translation="THLACTHDPIDGGIPKPFSMPVRDLQLRAGAGFVTVLTGELTTM
                     PALTRRPNAVDMEVDPATGKITGM"
     gene            complement(243..1205)
                     /locus_tag="VGB85_02595"
     CDS             complement(243..1205)
                     /locus_tag="VGB85_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132932.1"
                     /translation="MRREVILCAALVAVACSDHRYGFFGDEDGETTGSPDPTDGREPN
                     PDPTGVPGPVTVTTQSDPGEPDPPDPGEPDPTGVPGVCGERVLPSELPLRVEGDNSGG
                     PTFFGLTCGFGIASEAVFVWTAPFDGVFRFDTAGSSFDTVLAVTASYCGGPELGCNDD
                     ALGVFSAVTTLVPGGETVTIVVEGKTGEEGPVVLNIHEESLPCSFADIGSELGDLASS
                     TTFGPNVLEGSCGGLGANETPLLWRAPFSGLFRFETFGSAFDPVLYIRQDDCNGPEIR
                     CNDDREGVEAGFDLFVDGGQPLLVVVDGLGVGHAGDYTLRISAL"
     gene            complement(1213..2904)
                     /locus_tag="VGB85_02600"
     CDS             complement(1213..2904)
                     /locus_tag="VGB85_02600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0132933.1"
                     /translation="MAVRREVDDTDKASGEVATATAQPWLAALLTATKAAALRVQVMP
                     AVRILEVAGAVRALSGHEPEELRDNPSLLWRLVHPEDRQAYAQAVGAEDTQTAVVRWQ
                     RRRGGILWTEHTVTPWRDELEHAAGFSVMIREVTKRLEAQRELAHTREALEWARRDSA
                     YFHTLIHTVNDGILVNDADGTIEFVNAQLAGLLRATPDEMVGRHIFDYMDDESAATAR
                     ANLRRRSAGEEDQFDFRWRRKDGSEFWSIVAAKPLYDAEGRHRGSLVAVTDITRRKQA
                     EDALVRARNELEQRVAERTEALALEVQERRRAESDAQQASRTKSLFLANMSHELRTPL
                     NAILGYSELLIEESEDTESQQHIDIERIRGAADHLLGLINNILDLSKIEAAKMEVSSD
                     EFVIGELLNDVRSTILPLLIKGQNRLQITCNCEARSVVTDRTKLKQIVLNLLSNASKF
                     TQRGWVDLRMDLAWDEGVEWLTVQVADTGIGIPEHKLPNLFHVFAQADDSVARRYGGT
                     GLGLAISRELTRLLGGDIGVASEVGKGTTFTLRIPVLGTEYLDAAPGTTGGGMLR"
     gene            3076..>3759
                     /locus_tag="VGB85_02605"
     CDS             3076..>3759
                     /locus_tag="VGB85_02605"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0132934.1"
                     /translation="MQSAKLQLGTSTVSLDERTPRVLIGRDASCGLVTPEISVSRRHA
                     EVFLQGDLLLVRDLGSSNGTWINGRPVAADPSPVRPGDQIFLGHIPLAVEWEGGVQGK
                     TVVGELPADLRAMIEAHKNRVLTGGPVASAKSFLHDPPLPGGPAATMLGIGGREAPRA
                     AEFAYRRQGSNNNGVLLIALRSDTYSNGAEVDGFLEFTALDNETIASIIVELVEHHKK
                     GPKDGHVWDR"
BASE COUNT          542 a         1455 c         1198 g          564 t
ORIGIN      
        1 agacccacct cgcctgcacc cacgacccga tcgacggcgg catccccaag ccgttctcga
       61 tgccggtgcg cgacctccag ctgcgcgccg gcgccggctt cgtcaccgtg ctgaccggcg
      121 agctgacgac catgccggcg ctgacccgcc ggcccaacgc cgtcgacatg gaggtcgacc
      181 ccgcgaccgg caagatcacc ggcatgtgat cgccggggac atgtcctccc ggacatgtcc
      241 cctcagagcg ccgagatccg cagcgtgtag tcgccggcgt gcccgacccc gagtccgtcg
      301 acgaccacca gcaacggctg acctccgtcg acgaacaggt cgaagcccgc ctcgaccccc
      361 tcgcggtcgt cgttgcaccg gatctccggg ccgttgcagt cgtcctggcg gatgtacaac
      421 accggatcga aggccgaccc gaacgtctcg aagcggaaca gccccgagaa cggcgcccgc
      481 cacagcagcg gcgtttcatt ggcccccagc ccgccgcagc tgccctcgag cacgttcggc
      541 ccgaaagtcg tcgaactggc gaggtcgccg agctccgagc cgatgtcggc gaagctgcac
      601 ggcagcgact cctcgtggat gttgagcacc accggcccct cctcgccggt cttaccctcg
      661 accacgatcg tcaccgtctc gccgcccggc accagcgtcg tcaccgcgga gaacaccccg
      721 agcgcatcgt cgttgcagcc gagctcgggc ccgccgcagt agctcgccgt caccgccagc
      781 accgtgtcga agctcgagcc cgccgtgtcg aagcggaaca ccccgtcgaa cggcgccgtc
      841 cacacgaaca ccgcctcgct cgcgatcccg aagccgcagg tgagcccgaa gaaggtcggc
      901 ccgccgctgt tgtcgccctc gacgcgcagc ggcagctccg acggcaggac ccgctcgccg
      961 cagacccccg gcactccggt cgggtccggc tcgcccgggt ccggggggtc cggctcaccc
     1021 gggtccgact gcgtcgtcac cgtcaccggg cccggcacgc cggtcgggtc ggggttcggc
     1081 tcgcggccgt cggtcgggtc cggcgagccc gtggtctccc cgtcctcgtc gccgaagaag
     1141 ccgtagcggt ggtcgctgca ggcgacggcc acgagcgcgg cacacaggat cacttcacgg
     1201 cgcatgcaga cgtcagcgta acataccgcc gccggtcgtt cccggcgccg catcgaggta
     1261 ctccgtgccg agcacgggga tccgcagggt gaaggtcgta cccttgccga cctcgctggc
     1321 gaccccgatg tcgccgccga gcagccgcgt cagctcgcgg gagatcgcca gaccgaggcc
     1381 cgtgccgccg tagcggcgcg ccaccgagtc gtcggcctgc gcgaacacgt ggaacaaatt
     1441 gggcagcttg tgctccggga tgccgatgcc cgtgtccgcg acctgcaccg tcagccactc
     1501 gacgccctcg tcccacgcga ggtccatgcg caggtcgacc cagccgcgct gcgtgaactt
     1561 gctggcgttg ctgagcaggt tgaggacgat ctgcttgagc ttggtgcggt cggtgacgac
     1621 cgaccgcgcc tcgcagttgc acgtgatctg cagccggttc tggcccttga tcagcagcgg
     1681 caagatcgtg ctgcgcacgt cgttgagcag ctcgccgatc acgaactcgt ccgacgacac
     1741 ctccatcttg gccgcctcga tcttcgacag gtcgaggatg ttgttgatca gcccgagcag
     1801 gtggtccgcc gcgccgcgga tgcgctcgat gtcgatgtgc tgctgcgact cggtgtcctc
     1861 cgactcctcg atcagcagct ccgagtagcc gaggatcgcg ttgagcggcg tgcgcagctc
     1921 gtggctcatg ttggccaaaa acaggctctt ggtccggctc gcctgctgcg cgtccgactc
     1981 ggcccgccgc cgctcctgca cctcgagcgc cagcgcctcc gtccgctccg ccacccgctg
     2041 ctcgagctcg ttgcgcgccc gcaccagcgc gtcctcggcc tgcttgcgcc gcgtgatgtc
     2101 cgtgaccgcc accagcgagc cgcggtgccg gccctcggcg tcgtacagcg gcttggccgc
     2161 gacgatgctc cagaactccg agccgtcctt gcggcgccag cgaaagtcga actggtcctc
     2221 ctcgccggcg ctacgccggc gcaggttggc gcgcgccgtc gcggcgctct cgtcgtccat
     2281 gtaatcgaag atgtggcgcc cgaccatctc gtcgggcgtc gcgcgcagca gcccggcgag
     2341 ctgggcattg acgaactcga tcgtgccgtc ggcgtcgttg acgaggatcc catcgttgac
     2401 cgtgtggatc agcgtgtgaa agtacgcgct gtcgcggcgc gcccactcga gcgcctcgcg
     2461 ggtgtgggcc agctcgcgct gcgcctcgag ccgcttcgtg acctcccgga tcatcaccga
     2521 gaagccggcc gcgtgctcga gctcgtcgcg ccacggcgtc accgtgtgct ccgtccagag
     2581 gatccccccg cgacgccgct gccagcggac caccgccgtc tgcgtgtcct cggcgccgac
     2641 cgcctgcgcg tacgcctgac ggtcctccgg gtgcacgagg cgccacaaca acgacgggtt
     2701 gtcgcgcaat tcctccggct cgtgccccga cagcgcccgc accgcgccgg ccacctcgag
     2761 gatccgcacc gccggcatca cctgcacccg cagcgccgcc gccttcgtgg cggtcaacag
     2821 cgccgcgagc cacggctgcg ccgtcgcggt cgcgacctct cccgacgcct tgtccgtgtc
     2881 gtccacctcg cgccgaaccg ccatgcgcta cggtcacgct acgccatggc ggacccatag
     2941 aataccctcc atacgcgcgc gtgtggccac gaacaccgtt tgagctgcac catggcgcct
     3001 ggcgcgcctt cgcgccgatc tcttcccctc cctggcccgt tgactccgcg ggcatttgcg
     3061 gctgccatgg cgcccatgca gtcggcaaag ctccagctcg gcacctccac ggtttcgctc
     3121 gatgaacgca cccctcgcgt gctcatcggc cgcgacgcct cgtgcggcct cgtcactcca
     3181 gagatcagtg tgtcgcgccg ccacgccgag gtgttcttgc agggcgacct gctgctggtg
     3241 cgagacctcg gctcctcgaa cgggacgtgg atcaacggcc ggccggtcgc cgccgacccg
     3301 agcccggtcc gccccggcga ccagatcttc ctcggccaca tcccgctcgc ggtcgagtgg
     3361 gagggcggcg tgcagggcaa gaccgtcgtc ggcgagctgc cggccgacct ccgcgcgatg
     3421 atcgaggccc acaagaaccg cgtgctgacc ggcggacccg tcgcctcggc caagagtttc
     3481 ctccatgacc cgccgctgcc cggcggtccg gcggcgacca tgctcggcat cggcggacgc
     3541 gaggcccccc gggccgccga gttcgcctac cgccgccagg ggtccaacaa caacggcgtc
     3601 ctcctgatcg ccctgcgcag cgacacctac agcaacggcg ccgaggtcga cggcttcctc
     3661 gagttcaccg ccctcgacaa cgagacgatc gcctcgatca tcgtcgagct ggtcgagcac
     3721 cacaagaagg gccccaagga cggccacgtg tgggaccgg
//
LOCUS       DASWNJ010000141         5153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_108954, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000141 DASWNJ010000000
VERSION     DASWNJ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108954"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3820
                     /locus_tag="VGB85_02610"
     CDS             <1..3820
                     /locus_tag="VGB85_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132935.1"
                     /translation="TAPEPDPEPAPEPDPEPDPTPGPDLPPSPPPDESPGSPGAACLL
                     DADCGSDNFCELRICVTGCPVVALCDDDEVCDPHGRCATSESDEVQKGQHAGAPVLAD
                     RLTVLAFGETQARTILRNEGTEALTYRLAAENTALTLDNASATLAAGEEVELVVDVDV
                     TMPTPAERVLPVQIITSGGAIQWALMLDTWLSAGNFRGAVSFDIDGFSLGSSTLALDL
                     EFRDDGTIVGRNDDEASLLWPQPLALTGTWNTAGNVSIVLRDRLPADSWRHSPLAREL
                     GRELVLTGKRTATALAGTVTETITGLRAAPVAIEGTFSLHHRGPLTGIVHAPDFTPKD
                     AIAPTWLAPPDLDAEACDDLGIDYGTATKLLKPSLACNKCMDGTCSADDMYACGTALR
                     AAGYNLPPVLAALHGGGDVKPPTGWWTWEDCAAQVPEYNDERRACLDVAALHCAHALV
                     RRGPAQQPGALGDLFRSLAAVFAADEALAADLLATEAQIDTAFAFKDEVGEPLADAFA
                     RELAILTSDRERLAAALAPTLAPAYVDALAWIDDSDLDQPTANAHLAPLQLTADFART
                     TAQWARLAHRAGQNPDDVRAAVRLAMIAVHAAGVELHTRLAPNPAAAPGLHALGPALE
                     LLASIQDELASDATPFGYPTTYVPLALGPEDIAHNRSNFDAVQALAADEITQFSQVAA
                     DAWQKARDYELKTHTLAATELQISTEYNAKLRTLCGSLPGETTPALATCGEEGGQIAE
                     LRAAAKAAGLRIRHAAQAAENNLYAVVVEEERFGRVIEIQLGLAAEIAAAHGRIFQVK
                     DQYGEQRSAVEQAEALAECGRIRENSLAETEALQAGCDSQLVAQFTSGPTIFGVTIPN
                     VAGLIAAGAECDAKKASLATATQNQCKSVLGQAGLQSARDELQRSEEQEIMTINAEID
                     AAIRNSDLEVQRAASVALIKNIRAEGLLLQIETEEAELAQSTATTAMWSAYKEAAALV
                     QDQARTLGMMIEDSPDNALTRPHFLQARLDAAGRVLPSRERTIRRVYLALRALEYELN
                     QELPALRDTLATARSPEDITGLMTCLAGIAEDYRLEHGYGQPYVTEVSLRADIFGMTT
                     DIPDVDGSPATPAEQFAALLHDPLHGQADGGVALPFALSAFEHALFSSWLCDDRIDRI
                     EVKLVGDYLGDKEAEVLLTRQGLAGVRRCDGADLPQWSAYVPYSFEREQLVIQAGVNE
                     WGTAGPNAGYAAWPVHGEQWTLTIPPAEQAPANLDLDLAHVSDVVLRLHHRAGTIEPA
                     GQGTFTPGCG"
     gene            3825..4067
                     /locus_tag="VGB85_02615"
     CDS             3825..4067
                     /locus_tag="VGB85_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132936.1"
                     /translation="MAIPRIIPASALTPGLATIIVSLPGRTIRSRVFMVEQRPHGAVN
                     VAFASGDMVTLSARTMVGSIEYASHRPRSPRTRNAS"
     gene            4632..>5153
                     /locus_tag="VGB85_02620"
     CDS             4632..>5153
                     /locus_tag="VGB85_02620"
                     /inference="COORDINATES: protein motif:HMM:NF015497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HEY0132937.1"
                     /translation="MPTSFSRLLAVALVLGAPISARADTSATLATAINLPRGPASIEG
                     FGAGYDVSPASALPGMSFPLEVPPGRAGLAPELALHYHPGAGADVLGLGWSLRLPAIE
                     RSLRKGVPRYDDANTWTLKNLGDGEELVSVAPGIFRQRIELGPPVEIKEMPDGSMVAR
                     TVDGAEYYFGVTAD"
BASE COUNT          812 a         1949 c         1651 g          741 t
ORIGIN      
        1 gacggcgccc gaacccgacc ccgagccggc ccccgagccc gaccccgagc ccgaccccac
       61 tccgggcccc gatcttccgc cgtcgccgcc gcccgacgaa agccccggct cgcccggtgc
      121 cgcgtgcctg ctcgacgccg actgcggctc ggacaacttt tgcgagctgc ggatctgcgt
      181 caccggctgt cctgtcgtgg ccctgtgcga cgacgacgag gtctgcgacc cgcatggtcg
      241 ctgcgctaca tccgagagcg acgaggtcca gaagggccag cacgctggtg cgccggtgct
      301 cgcggaccga ttgacggtcc tcgcgttcgg cgagacccag gcgcgcacca tcctccgtaa
      361 tgagggcacg gaggcgctga cgtaccgcct cgcggccgag aatactgcgc tgacgctgga
      421 taacgcgtcg gcaacgctcg cggcggggga agaggtcgag ctggtcgtgg acgtcgatgt
      481 cactatgccc acgccggccg agcgggtgct gccggtccag atcatcacca gcggcggggc
      541 catccagtgg gcgctcatgc tcgatacatg gctctcggcc ggcaacttcc gcggcgccgt
      601 aagcttcgac atcgatggct tcagcctagg gagcagcacc ctcgccctcg atctcgaatt
      661 tcgcgacgac ggcacgatcg tgggtcgcaa cgacgacgag gcgagcctcc tgtggccgca
      721 accgctggcg ctgactggca cctggaacac cgccggcaat gtctcgatcg tgctgcgcga
      781 ccgcctgccc gccgacagct ggcgccacag cccgctcgcg cgcgaactgg gccgcgagct
      841 cgtgttgacc ggcaagcgca cagccaccgc cctcgcgggc accgtgacgg agacgatcac
      901 gggcctgcgt gccgcaccgg ttgcgatcga gggcacattt tcactgcatc accgcggccc
      961 actcacaggc atcgtccacg cacccgactt cacccccaag gacgccatag cgccgacctg
     1021 gctcgcacct ccagatcttg acgcggaggc gtgcgacgac ctcggcatcg actatggtac
     1081 cgccacgaag ctgctcaagc cctccctcgc gtgtaataag tgcatggacg gtacctgctc
     1141 ggccgacgac atgtatgcat gcggcacggc gctacgggcc gccggttaca acctcccgcc
     1201 cgtgctcgcc gccttgcatg gcggcggcga cgttaagcct cccaccggat ggtggacctg
     1261 ggaggactgc gccgcgcagg ttcccgagta caacgacgag cgtcgggcct gcctggacgt
     1321 cgccgcgctg cactgcgccc acgcgctggt acgacgcggg cccgcccagc agcccggcgc
     1381 cctgggcgac ctgtttcgca gcctcgccgc cgtgttcgcc gcggacgagg ccctcgccgc
     1441 cgatctcctg gcgaccgagg cccaaatcga caccgcgttc gcgttcaagg acgaggtcgg
     1501 cgagccgctc gcggatgcgt tcgcgcgcga gttggcgatc ctcacctcgg atcgcgagcg
     1561 cctcgccgcc gcgctggccc cgacgctcgc gccggcctac gtcgacgccc tcgcgtggat
     1621 cgacgactcc gaccttgatc agcccacggc caacgcccac ctcgcgccct tgcagctcac
     1681 cgccgacttc gcgcgcacca cggcgcagtg ggcccggctg gctcaccgtg cgggccagaa
     1741 ccccgacgac gtgcgagccg cggtgcggct ggcgatgatc gccgtccatg ccgcgggtgt
     1801 cgagctgcat acacgcctcg cgcccaaccc cgcggccgcg ccgggtctgc acgcgctcgg
     1861 cccggcgctg gagctgctgg catcgatcca ggatgagctg gcatcggacg caaccccctt
     1921 cggctacccg acgacctacg tcccgctcgc gcttggcccc gaagacatcg cccacaatcg
     1981 cagcaacttc gacgccgtcc aggcgctcgc cgccgacgag atcacacagt tctcgcaggt
     2041 cgccgcggac gcctggcaaa aggcccgcga ctacgagctg aagacccaca cgctcgcggc
     2101 caccgagtta caaatctcga cggagtacaa cgccaagctg cgtacactgt gtggcagcct
     2161 acccggcgag acgaccccgg cccttgcgac ctgtggcgag gagggcgggc agatcgccga
     2221 gctgcgcgcg gccgccaagg ccgccggtct acgcatccgc cacgccgccc aggccgccga
     2281 gaacaacctg tatgcggtcg tggtcgagga ggagcgtttc ggccgtgtca tcgagatcca
     2341 gctgggtctc gccgcagaga tcgcggccgc gcacggccgc attttccagg tcaaggatca
     2401 gtacggcgag cagcgctccg ccgtcgagca ggccgaggcc ctggccgagt gcggccgcat
     2461 ccgcgagaac agcctcgccg aaactgaggc gctccaggcc ggttgtgaca gccagctggt
     2521 cgcccagttc acctcgggtc cgacgatctt cggcgtcacg atacccaacg tcgccggcct
     2581 catcgccgcc ggggcggagt gcgatgccaa aaaggcgagc ctggcgacag cgacccagaa
     2641 ccagtgcaag agtgtgctgg gacaggcggg cctccagagc gcgcgcgacg agctccagcg
     2701 cagcgaggag caagagatca tgacgatcaa cgccgagatc gacgcggcga tccgcaacag
     2761 cgatctcgag gtccagcgcg ccgcgtcggt cgctctcatc aagaacattc gagccgaggg
     2821 cctgctgttg cagatcgaga ccgaggaggc cgagctggcg cagagcaccg ccacgaccgc
     2881 catgtggagc gcctacaagg aggccgccgc gctcgtccag gatcaagccc gcacgctggg
     2941 catgatgatc gaggacagcc cggacaacgc cctgacgcgc ccgcatttcc tgcaagctcg
     3001 cctcgatgcc gccggccgcg tcctgccgtc gcgcgagcgg acgatccgcc gggtgtacct
     3061 cgcgctgcgc gccctggagt acgagctgaa tcaggagctg ccggcgctgc gtgacacgct
     3121 cgccacggcg cgctcgcccg aggacatcac cggcctgatg acctgcctcg ccgggatcgc
     3181 ggaggactac cgcctcgagc acggctacgg ccagccttac gtcaccgagg tcagcctgcg
     3241 cgccgacatc ttcggcatga ccaccgacat cccggacgtc gacggcagcc ccgcgacccc
     3301 ggccgagcaa ttcgcggcgc tcctgcacga tcccttgcat ggccaagcgg acggcggagt
     3361 cgcgctgccc ttcgcgctct cggcgttcga gcacgccctg ttttcaagct ggctgtgcga
     3421 cgacaggatc gaccggatcg aggtcaagct cgtcggcgac tacctcggcg acaaggaggc
     3481 cgaggtgttg ctgacgcgcc agggcctcgc gggcgtgcgc cgctgcgacg gcgccgacct
     3541 cccgcagtgg tccgcgtacg tgccctacag cttcgagcgc gagcaactcg tcatccaagc
     3601 cggcgtcaac gagtggggca ccgcggggcc gaacgccgga tacgccgcct ggcccgtgca
     3661 tggcgagcag tggaccctca cgatcccacc ggccgaacag gcgcccgcga acctcgacct
     3721 cgacttggcg cacgtctccg acgtcgtgct gcgtctgcat caccgcgccg gcacgatcga
     3781 gccggcgggc cagggcacct ttaccccggg ctgcgggtga cgacatggcc atcccgcgca
     3841 tcatccccgc ctccgcgctg acgcccggtc tcgccacgat catcgtctcg ctccccggcc
     3901 ggaccatccg cagccgcgtg ttcatggtcg agcagcggcc ccacggggcc gtgaacgtcg
     3961 cgttcgcctc cggcgacatg gtcacgctct cggcgcggac catggtcggc agcatcgagt
     4021 acgcctcgca ccggccacga tcgccgcgca cccgaaacgc ctcctgaggg cgggacgaga
     4081 aagccagtca tgtcgctcga agcacccacg agctcatcac cggatggcac gcccggtccc
     4141 agccagcggt cctggccgac atttccgccg acgatgctgc agcgccttcc gaagttcttg
     4201 ccgaaaaact tacggcaagc ctttcgggcg agacacggcc accgacgcgc ggagcatggc
     4261 ggccaccggc ccgccggctc acgccgcgcg gatttccgcg ctgcgcatct tcttcgagcc
     4321 ggctgtgtca gtcgagtctg gcgggtcggg tccgtcgggt ctgcctggtc ggcctgcggt
     4381 cgaaactccg ggattacggc gggccgcggc catggtccaa ctttccaaag gcaccggacg
     4441 aacatgtccg acgcattcgc ctgcggtgca gggattttct cccgcgagac ccgaccccct
     4501 ctgcccgccg ctggaaatgc ggcgccggct ctgctcgccg ccgtgccgca gcctgactgc
     4561 gccctccatt ttccggctcg ctcgccgcga cctttcgcgc cgcgacgggc ctccaccgcc
     4621 ccgtgaactc catgcccaca tctttttccc gcctcctcgc cgtcgcgctc gttctgggcg
     4681 cgccaatttc cgcccgcgcc gacaccagcg cgacgctcgc caccgccatt aatctcccgc
     4741 gcggtcctgc ctcgatcgag ggcttcggcg cgggctacga cgtcagcccc gcgagcgcgc
     4801 tccccgggat gtccttcccg ctcgaggtcc cgcccggacg cgccggcctc gcgccggagc
     4861 tggcgctgca ctaccacccc ggcgcaggcg cggacgtgct cggcctcggc tggtcgctgc
     4921 gcctgcccgc gatcgagcgg tcgctccgca agggcgtccc gcgctacgac gacgctaaca
     4981 cctggaccct taaaaatctc ggcgacggcg aagaactcgt ttccgtggcg cccggcattt
     5041 tccgccagcg gatcgagctg gggccgcctg tggaaattaa agaaatgccg gacggcagca
     5101 tggtcgcccg cacggtggac ggcgccgaat attattttgg cgtcaccgca gac
//
LOCUS       DASWNJ010000142         6748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_250002, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000142 DASWNJ010000000
VERSION     DASWNJ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6748
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250002"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="VGB85_02625"
     CDS             <1..1131
                     /locus_tag="VGB85_02625"
                     /inference="COORDINATES: protein motif:HMM:NF040941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-like YCDxxxxGGGW domain-containing
                     protein"
                     /protein_id="HEY0132938.1"
                     /translation="NNSNTGACTLACKLPVCGDQFVQPPEQCDDGNLSDNDACLSTCK
                     GATCGDGKVFQGQEQCDDGNQSNNDQCTVTCKTATCSDGFKNGAETDVDCGGPTCNKC
                     ELAKACLLPGDCQSGSCINGKCSDPTSCKQIKQGNPAAQSGFYTIDPDGPGGQAAFLA
                     RCDMVADGGGWTLIGSQVNADARAWTTLAIHTGASNFGDVNSYTTADYKNVGWATIPA
                     RNLLLRTEEYAVGWTNNVLQDTGFGPWINGKYNINMCSQQFIGGTPAYGENITASQRS
                     IFDIAVRALDSNCECFPGCNENALVTFTLQSCCWTMGIGNAPQGQVTWSGHDQSLLKL
                     SSLVAQACNPAVYPCNPQGFWNAGTPCYDTTCKTKYTSVWVR"
     gene            complement(1218..1769)
                     /locus_tag="VGB85_02630"
     CDS             complement(1218..1769)
                     /locus_tag="VGB85_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132939.1"
                     /translation="MTFSLSALVPASLVKLLPALLVPTLMAVASTAQAGLDITYGYTS
                     AMTQTTNGFDYYVEAEGTFTSANGTRDTSLSMDCSVNTILESSACIGTMTLGTVTINI
                     SNGFDGVTGMVETSMPSNLPPSLDIVHDFWMRDTMPHFHYFPGLEAIDIVVNGQIRHW
                     FYVSSWADEVANYTDFDPNAIWK"
     gene            complement(1923..2660)
                     /locus_tag="VGB85_02635"
     CDS             complement(1923..2660)
                     /locus_tag="VGB85_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132940.1"
                     /translation="MHGSAIALLFVTYLTALGVAMWTDVPAQTLITWGAGGAALLWLI
                     VLVTLPWNLHLRAREVLAEMRRSREAGIVVTDEQLAFAEKVKRWTLLTSLGTHVLSAA
                     VALLVARFADAPLGYPFAGFYLLSCVVRPASAAMQQLRRRLETIAADTRYPRDDVLKL
                     RAQVEAHEAAVEQHKERLRELDARNEALGAQLERQRLDLEQRMTALTRKFEESLDRLT
                     DNRELIAGIKAFLRMIRGDSDPAAGGS"
     gene            3003..3902
                     /locus_tag="VGB85_02640"
     CDS             3003..3902
                     /locus_tag="VGB85_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY0132941.1"
                     /translation="MASKDSPVILVSIAAATAITITKFVAAILSGSSGMVSEGLHSLA
                     STVRSVLLLVGSRMSRRKSDSLHPFGHGKELFFWTLIAVLLVIIVAGAGSIYQGIVKL
                     VERESIADVEFNYIVLGIAGALGIAGWIPAIREVRSQRGGRSVWQTLWNMRDTATLAI
                     YLDGVASVIALVLATLGIYLSDTHLEPAYDGVAAILIGVMWFVVLVPLVQRCRQLLVG
                     EGAEDETLASITEMVAADPDVRQVVRSRAMSFGVEDLLVSIGLRFREDLSASELGQVI
                     RRIEDRLREHHDGLKHISIEAHP"
     gene            4017..5285
                     /locus_tag="VGB85_02645"
     CDS             4017..5285
                     /locus_tag="VGB85_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY0132942.1"
                     /translation="MARLNTEHNERRVLVARALENERRVTAVRMASLVLMGLSQDVIV
                     GLSGEAAEEDPAQAVAIGLYVAFTIAAFIGVRRVTESVMTVRVMPFIAATFDVGFILA
                     MDITEAGYGAIHLEGTATMLALVISYSLLRYGIESLVYSTTLAIGAFTAAMRLAEQFS
                     WGRWSFVVFGFIASAVLLGFTRVAVRRAFLDLQRRELLSRLVAPKVVDEILAGRESSL
                     RPTRREVTVLFADIRDFTTFSEAREPEEVLRFLDDYFGRMTQVVHGREGSVNKFLGDG
                     LMALWGAPEPLADHAVHAVRAALDMQKVMREINERRVADGEPPLRIGIGVHTGAVAAG
                     MLGSSSQSEYSVIGDAVNLASRIEGLTREYAAEILVSDATWHSLGGQFTGERLGEVTV
                     KGRRAGVVIHALHPLPATDLLDTPAGSQPQ"
     gene            5289..>6748
                     /locus_tag="VGB85_02650"
     CDS             5289..>6748
                     /locus_tag="VGB85_02650"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132943.1"
                     /translation="MPGLALALPLLLAAGPRPPVARVADEVTVTLHGEPRHDEYAWMR
                     AGADDPAVRAHLGAENAYAQALLRPHQPLRRALAREIEARAYIDHESAPVRRGAWLYW
                     TRQSRRADHPVYLRRPAASPDAPPQVVLDVERLARGKPFFDLGLFELSEDGRWLAYAT
                     DETGHEDYTLFIRDLETGALAPDRLLRVTSAAWTAGARELLYTVRDDTHRSYALWSHK
                     RGEAGGDRLVFTETDPAFDLSIARSRSGEWLFLASDSYLASEVRVARAEQSAGPWKII
                     AARQRGHLYELDHSGDLFYVRSNYAAPNFHVMTAPEDDPRPARWRRFVPERADVAITG
                     VDAFAEHVVLSQREQGQPRLAIVERATGTMIRPGDVDAARGQIPRTQGLVGLWPGENP
                     DPRATVFRVHVESVHEDEVTVDVALRDGAETVVGRRATQPCGGVARSVGTASAPDGTQ
                     ISYLYFGGSDPGGGPRPLLLEAYGAYGSTYDPDFDPS"
BASE COUNT         1035 a         2286 c         2402 g         1025 t
ORIGIN      
        1 aacaacagca acaccggcgc gtgcacgctc gcgtgcaagc tcccggtgtg cggcgaccag
       61 ttcgtgcagc cgcccgagca gtgcgacgac ggtaatctct ccgacaacga cgcgtgcctg
      121 agcacctgca agggcgcgac ctgcggcgac ggcaaggtgt tccagggcca ggagcagtgc
      181 gacgacggca accagagcaa caacgaccag tgcacggtga cgtgcaagac ggcgacctgc
      241 tcggacggct tcaagaatgg cgccgagacg gacgtcgact gcggcggtcc gacctgcaac
      301 aagtgcgagc tggccaaggc gtgtttgctc cccggcgact gccagagcgg cagctgcatc
      361 aacggcaagt gcagcgaccc gaccagctgc aagcagatca agcagggcaa cccggccgcg
      421 cagagcggct tctacacgat cgaccccgac ggccccggcg gtcaggccgc gttcctcgcc
      481 cgctgcgaca tggtcgcgga cggcggcggc tggacgctga tcggctcgca ggtcaacgcc
      541 gacgcgcgcg cctggaccac cctggcgatc cacaccggcg cgagcaactt cggcgacgtc
      601 aacagctaca cgaccgccga ctacaagaac gtcggctggg cgacgatccc ggcgcgtaac
      661 ctgctcctgc gcaccgagga gtacgcggtc ggctggacca acaacgtgct gcaagacacc
      721 ggcttcggcc cgtggatcaa cggcaagtac aatatcaata tgtgctcgca gcagttcatc
      781 ggcggcacgc cggcctacgg cgagaacatc accgccagcc agcgcagcat cttcgacatc
      841 gccgtccgcg cgctcgacag caactgcgag tgcttccccg gctgcaacga gaacgccctc
      901 gtcaccttca cgctgcaaag ctgctgctgg acgatgggca tcggcaacgc accgcaaggc
      961 caggtcacct ggtcgggcca cgaccagtcg ctgctcaagc tcagctcgct ggtcgcccag
     1021 gcgtgcaacc ccgccgtgta cccctgcaac ccgcagggct tctggaacgc gggcacgccc
     1081 tgctacgata cgacctgcaa gacgaagtac acgagcgtct gggtccgctg agataagccc
     1141 gggtttacgc tggcgtcgag gtcacctgct gggcaggggc cggcgtcggc ggggcacagc
     1201 gggccggcga cccgcgacta cttccagatc gcgttcgggt cgaaatcggt gtagttggcg
     1261 acctcgtcgg cccaggacga gacgtagaac cagtggcgga tctggccgtt gacgacgatg
     1321 tcgatcgcct ccaggcccgg gaagtaatga aagtgcggca tggtgtccct catccagaaa
     1381 tcgtgaacga tgtcgagtga gggcgggagg ttgctgggca tcgaggtctc gaccatgccg
     1441 gtgacgccgt cgaagccgtt gctgatgttg atggtcacgg tccccagcgt catggtcccg
     1501 atacacgcgc tgctctccag gatggtgttc accgagcagt ccatcgacag ggaggtgtcg
     1561 cgggtcccgt tcgcgctggt gaaggtgccc tcggcctcga cgtagtagtc gaagccgttc
     1621 gttgtctgcg tcatcgccga ggtgtagccg taggtgatgt cgaggccggc ctgcgcggtg
     1681 ctggcgacgg ccatcagggt ggggaccagc agggcaggca ggagcttgac gagcgaagcg
     1741 gggacaaggg cggacaaaga aaaggtcatg acgatctcgc ttggatgggt gggcgttcga
     1801 ccggggcgga cgccggggtc gttcgtgggc ccattgccag cgagctggcg agcgctgaca
     1861 tgcccgtgca aaaaaatagt ccggggcgat gcgcggtccc gtcgcgtcgg cgggcgcgcg
     1921 ggtcacgatc cgccggcggc cgggtcgctg tcgccgcgga tcatccgcaa aaacgccttg
     1981 atgccggcga tcagctcgcg gttgtcggtc aggcggtcga ggctctcctc gaacttgcgc
     2041 gtcagcgcgg tcatgcgctg ctcgaggtcg aggcgctggc gctcgagctg ggcgccgagc
     2101 gcctcgttgc gggcgtcgag ctcgcgcagc cgctccttgt gctgctcgac ggcggcctcg
     2161 tgggcctcga cctgggcgcg cagcttgagc acgtcgtcgc gcgggtagcg ggtgtccgcg
     2221 gcgatcgtct cgaggcgccg ccgcagctgc tgcatcgccg cgctcgccgg acgcaccacg
     2281 cagctgagca gatagaagcc cgcgaagggg tagccgagcg gcgcgtcggc gaagcgcgcg
     2341 acgagcagcg cgaccgcggc ggacagcacg tgggtgccga ggctggtcaa cagggtccag
     2401 cgcttgacct tctccgcgaa cgcgagctgc tcgtcggtga cgacgatccc cgcctcccgc
     2461 gagcgccgca tctcggcgag cacctcgcgc gcgcgcaggt gcaggttcca cggcagcgtc
     2521 accagcacga tcagccacaa caacgccgcc ccgccggccc cccaggtgat cagggtctgc
     2581 gccggtacat ccgtccacat cgccacgccg agcgcggtga ggtaggtgac gaagagcaac
     2641 gcgatcgccg agccgtgcat ggtcgcgagc gtactggaat gccgcgcgcg atggtgtcgc
     2701 cgggcgcgcc ctgccacgag atgacctgca agtacacgag cgtccgggtc cgccgaggta
     2761 aatcggcggt atcgagggcg ctcccgcgac cgtgggggcg ccgtttgtcg tgcgagcaat
     2821 tggacgaatt cgccgaggcg cgagcgcgct cctcggcgcg cgcgccggcc gtggtggtgc
     2881 ctgcgccggc cgtggcgata cgcgctcgcg tgtccgcgag gatcggccgc cgcggcgagc
     2941 gtcgcctggc tcgctggcac gggcgggggt catggcatat gcgccggcga ctcgtcgcag
     3001 ccatggcctc caaagattcc ccagtcatcc tcgtaagcat cgccgcggcg accgccatca
     3061 cgatcacgaa attcgtggcg gccattctct ccggtagctc ggggatggtg tcggaggggt
     3121 tgcattcgct cgccagcact gtccgcagcg tcctgctgct ggtcggatcg cggatgagcc
     3181 ggcgcaagag cgactcgctg cacccgttcg ggcacggcaa ggagctgttc ttctggacct
     3241 tgatcgcggt gctgctggtg atcatcgtcg ccggcgccgg ttcgatctat cagggcatcg
     3301 tcaagctcgt cgagcgcgag tcgatcgccg acgtggaatt caattacatc gtactcggga
     3361 tcgccggggc cctgggcatc gcggggtgga tcccggcgat ccgcgaggtc cggtcgcagc
     3421 gcggcggacg cagcgtgtgg cagaccctct ggaacatgcg cgacaccgcg acgctggcga
     3481 tctacctcga cggcgtcgcg tcggtgatcg ccctggtgct cgcgacgctc gggatctatc
     3541 tcagcgacac gcacctcgag ccggcctacg acggcgtcgc tgcgatcctc atcggggtga
     3601 tgtggttcgt cgtgctcgtg ccgctggtcc agcgctgccg tcagctgctc gtcggcgagg
     3661 gcgcagagga cgagacgctc gccagcatca ccgagatggt cgccgccgac ccggacgtca
     3721 ggcaggtcgt gcgctcgcgg gcgatgagct tcggcgtcga ggatctgctc gtcagcatcg
     3781 gcctgcggtt ccgcgaggac ctgtcggcga gcgagctggg ccaggtgatc cgccgcatcg
     3841 aggacaggct gcgcgagcat cacgacggcc tcaagcacat ctcgatcgag gcccacccgt
     3901 gacgcgccct tgctgaggtc ttccgtgcgc ggatgtgcgc gcttcgtcgg gcgccgcggc
     3961 aggctatcct cgcggcgttg ccagctccgg tccgcgacga aaccaggctg aggacgatgg
     4021 cccggctcaa caccgagcac aacgagcgtc gggtgctcgt cgcccgcgcg ctggagaacg
     4081 agcgccgggt gacggcggtg cggatggcct cgctggtgct gatgggcctc agccaggacg
     4141 tcatcgtggg gctctcgggc gaggccgccg aggaggatcc ggcgcaggcg gtcgcgatcg
     4201 gcctctacgt ggccttcacg atcgcggcct tcatcggggt gcggcgggtc accgagagcg
     4261 tgatgaccgt gcgggtgatg cccttcatcg ccgcgacctt cgacgtcggc ttcatcttgg
     4321 cgatggacat caccgaggcc gggtacggcg cgatccacct cgagggtacg gcgacgatgc
     4381 tggcgctggt gatcagctac tcgctgctgc gctacgggat cgagagcctg gtgtactcga
     4441 ccacgctggc gatcggggcg ttcaccgcgg cgatgcgact ggcggagcag ttttcctggg
     4501 ggcgctggag cttcgtggtg ttcggcttca tcgcgtcggc ggtcctgctc gggttcacgc
     4561 gcgtcgcggt gcgccgcgcc ttcctcgacc tccagcgccg cgagctgctg tcgcgcctcg
     4621 tcgcgcccaa ggtcgtcgac gagatcctcg ctggccgcga gagcagcctg cgcccgaccc
     4681 ggcgcgaggt caccgtgctg ttcgccgaca tccgcgactt cacgaccttt agcgaggcgc
     4741 gcgagccgga ggaggtgctg cgcttcctcg acgactactt cggccgcatg acgcaggtcg
     4801 tccacgggcg cgaggggtcg gtcaacaagt tcctcggcga cgggctgatg gccctgtggg
     4861 gcgccccgga gccgctcgcg gaccatgccg tgcacgcggt gcgggcggcc ctcgacatgc
     4921 aaaaggtcat gcgggagatc aacgagcgcc gcgtcgccga cggcgagccg ccgctgcgga
     4981 tcgggatcgg cgtgcacacc ggcgccgtcg cggccggcat gctcggcagc agcagccaga
     5041 gcgagtacag cgtgatcggc gacgcggtca acctcgcctc gcgcatcgag gggctgaccc
     5101 gcgagtatgc ggcggagatc ctcgtcagcg acgcgacctg gcacagtctc ggcgggcagt
     5161 tcacaggtga gcggctcggc gaggtcacgg tcaagggccg gcgcgcgggg gtcgtgatcc
     5221 atgcgttgca cccgctgccg gcgacggatc tgctggacac gcccgccggc tcgcagccac
     5281 agtgacgcat gcccggcctc gcactcgccc tcccgctgct gctcgcggcc gggccgcggc
     5341 cgccggtcgc ccgcgtcgcc gacgaggtca cggtgacgct gcacggcgag ccgcgccacg
     5401 acgagtacgc ctggatgcgc gccggcgccg acgaccccgc cgtgcgcgcg cacctggggg
     5461 ccgagaacgc gtacgcgcag gcgctgctgc gcccgcatca accgctgcgc cgcgcgctcg
     5521 cgcgggagat cgaagcccgc gcatacatcg atcacgagag cgcgccggtg cgccgcggcg
     5581 cgtggctgta ctggacccgc cagtcgcggc gcgccgacca ccccgtgtat ctgcgacgac
     5641 cggcggccag cccggacgcg cccccgcagg tggtgctcga cgtcgaacga ctcgcgcgcg
     5701 gcaagccgtt cttcgacctc ggtctatttg aattgtccga ggacggccgc tggctggcct
     5761 acgcgaccga cgagaccggc cacgaggact acactctttt cataagagat ctcgagacgg
     5821 gcgcattggc gccggatcgg ctgctgcgtg tgacctcggc ggcgtggacg gcgggcgcgc
     5881 gcgagctgct gtacacggtg cgggacgaca cccaccggtc gtatgcgttg tggtcgcata
     5941 aacggggcga ggccggcggc gatcgcctgg tcttcaccga gacggacccc gccttcgacc
     6001 tgtcgatcgc acgcagccgc tccggtgagt ggctgttcct cgccagcgac tcgtacctcg
     6061 cctcggaggt ccgggtcgcc cgcgcggagc agtcggcggg tccgtggaaa attatcgctg
     6121 cgcgccagcg cgggcacctc tacgaactcg accattccgg cgacctgttt tatgtgcgta
     6181 gtaattacgc ggccccgaat ttccacgtaa tgacggcccc ggaggacgac ccccggccag
     6241 cgcggtggcg gcgcttcgtg cccgagcgcg ccgatgtcgc gatcaccggg gtcgacgcgt
     6301 tcgcggagca cgtcgtgctc tcgcagcgcg agcaggggca gccgcgcctg gcgatcgtcg
     6361 agcgggccac cggtacgatg atccgccccg gtgatgtcga cgcggcccgg ggtcagatcc
     6421 cgcgcacgca ggggctcgtg gggttatggc cgggtgagaa ccccgacccg cgggcgacgg
     6481 tgtttcgcgt gcacgtcgag tcggtgcacg aggacgaggt caccgtcgac gtcgcgctgc
     6541 gcgacggcgc cgagacggtc gtcggccgcc gcgcgacgca gccctgcggg ggcgtcgcgc
     6601 gctcggtcgg caccgcgagc gcccccgacg gcacgcagat ctcctatcta tatttcggcg
     6661 gaagtgaccc cggcggcgga ccgcggccgc tgctgctcga ggcctacggc gcctacggat
     6721 cgacctacga ccccgacttt gacccttc
//
LOCUS       DASWNJ010000143         2616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_462093, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000143 DASWNJ010000000
VERSION     DASWNJ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462093"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VGB85_02655"
     CDS             complement(<1..251)
                     /locus_tag="VGB85_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132944.1"
                     /translation="MTTRSLFALTLCLVTACTGSGAPAQVAPKLEDKPTTAAPAEPAE
                     PALPDAEKLLAESVEAMGGADKFAGLKSFYAESTLNMGAL"
     gene            complement(248..1651)
                     /locus_tag="VGB85_02660"
     CDS             complement(248..1651)
                     /locus_tag="VGB85_02660"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0132945.1"
                     /translation="MRQPPRGIFKTAALGGLLMVACGAEPAPVVKPATPTAAVEAAAP
                     VPKDSCPSWATLDTKSLPTLPETPHTATFEQVWRIVLEKHFDPTLGCQDWPARRVEFG
                     TKLGEARDAAGAYATMNGMLGTLKQSHFKVVPPSGATAPSAASGPARAPIHVRKIGDE
                     VVVVDAALGGVDSGVPRGAVLVAIDDKPVAEIVAKVEAEALRPDEVAFHAMLAVEAAM
                     HGVTGAKRKITFKDPSKGDAEVTREVACVDPAGELVTIGNLQNIPTTVTHRMMPGTKV
                     GHLAFNYWMLPMVKRVEAAMTELRAQGMTALVLDLRGNPGGVGAMAIPVARMLLKEAG
                     SLGKLQFRDFAQEFNVAANPDAFTGPVIVLVDEGTASTSEIFAAGLRDLGRVQIYGSR
                     PSAGAALPSMIEQLSGGAILQYVVGDYHSSKGAVAEGDGVKPDVTVPETRADFIAGRD
                     PVLAAATAALTTPEVKP"
     gene            complement(1653..2360)
                     /locus_tag="VGB85_02665"
     CDS             complement(1653..2360)
                     /locus_tag="VGB85_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0132946.1"
                     /translation="MPIEAAEIEQVRQALVRYAGWDQVPDEVMVELCRGAELIEVKRK
                     RSVFRRGEPGPGLFVVKSGEFKLSLISSEGREQILYLAEPGKLIGEGFLPKDVQCASS
                     AFAMLDSEAWQFETDHVINLIGRSQPLAFALMRHMAFRANRLIDLVLDLSLRSVERRL
                     ASFLLSLAVRAGAETGKSCVIPRQLDMNTVAARLGTVREEISRALNRMQRDGILSLSR
                     QEIVVLDLNALERVTYE"
BASE COUNT          411 a          915 c          885 g          405 t
ORIGIN      
        1 agcgcgccca tgttgagcgt cgactccgca taaaaactct tgagaccggc gaacttgtcc
       61 gcaccgccca tcgcctccac cgactccgcc agcagcttct cggcgtccgg caacgcgggc
      121 tcggccggct cggccggcgc cgcggtcgtc ggcttgtcct cgagcttcgg ggcgacctgc
      181 gccggggcac cggagcccgt gcaggcggtg acgagacaca acgtgagcgc gaacagggag
      241 cgagtcgtca tggtttaacc tcgggagtgg tcagcgccgc ggtggcggcg gcgagcaccg
      301 ggtcgcggcc ggcgatgaag tcggcgcggg tctcgggcac ggtgacatcg ggcttcacgc
      361 cgtcgccctc ggcgaccgcg cccttggagc tgtgatagtc gccgaccacg tattgaagga
      421 tcgcgccgcc gctgagctgc tcgatcatcg agggcagcgc ggcgccggcc gagggtcggg
      481 acccgtagat ctgcacgcgg ccgaggtcac gcagaccggc cgcgaagatc tccgaggtgc
      541 tcgcggtgcc ctcgtcgacg agcacgatca ccggcccggt gaaggcgtcc gggttggcgg
      601 cgacgttgaa ctcctgggcg aagtcgcgaa actgcaattt gccgaggctg ccggcctcct
      661 tgagcagcat acgcgcgacg gggatcgcca tcgcgccgac gccgcccggg ttgccgcgca
      721 ggtcgagcac cagcgccgtc atgccctgcg cgcgcagctc ggtcatcgcc gcctcgacgc
      781 gcttgaccat cggcagcatc caataattga acgcgaggtg cccgaccttg gtccccggca
      841 tcatgcgatg cgtgacggtg gtcgggatat tttgcagatt cccgatcgtc accagctcgc
      901 ccgccgggtc gacgcaggcg acctcgcgcg tgacctcggc gtcgcccttc gacggatctt
      961 taaaagtgat cttccgcttc gcgccggtga caccgtgcat cgcggcctcg accgcgagca
     1021 tcgcgtgaaa cgcgacctcg tcggggcgca gcgcctcggc ctcgaccttg gcgacgatct
     1081 cggcgaccgg cttgtcgtcg atcgccacga gcacggcgcc acgcggcacg ccgctgtcga
     1141 ccccgccgag cgccgcatcg acgacgacga cctcgtcacc gatcttcctg acgtggatcg
     1201 gtgcacgcgc cgggcccgag gcggcgctcg gggccgtcgc gccgctcggc ggcacgacct
     1261 tgaagtggct ctgcttgagc gtgccgagca tcccgttcat cgtcgcgtac gcacccgcgg
     1321 cgtcgcgcgc ctcgccgagc ttggtcccga actcgacgcg ccgcgccggc cagtcctgac
     1381 agccgagcgt cgggtcgaag tgcttctcga ggacgatgcg ccacacctgc tcgaaggtcg
     1441 cggtgtgcgg cgtctccggc agcgtgggca ggctcttggt gtcgagcgtg gcccacgacg
     1501 ggcagctgtc cttcgggaca ggcgcggccg cctcgaccgc agccgtgggc gtggcaggct
     1561 tcacgaccgg agccggctcc gcgccacacg cgaccatcag gaggccgccg agcgcggccg
     1621 ttttaaaaat tccccgcggt ggctggcgca cgttactcgt aggtgacgcg ctcgagggcg
     1681 ttgaggtcga ggaccacgat ctcctggcgg ctgaggctca ggatcccgtc gcgctgcatg
     1741 cggttgaggg cgcgggagat ctcctcacgc accgtaccga ggcgggcggc aacagtgttc
     1801 atgtcgagct ggcgggggat gacgcagctc ttgcccgtct cggcgccggc gcggacggcc
     1861 agcgacagca ggaaggaggc gaggcgccgc tccaccgagc gcagcgagag gtcgaggacc
     1921 aggtcgatca ggcgattggc gcgaaacgcc atgtgccgca tcaacgcgaa ggccagcggc
     1981 tgcgagcgac cgatcaggtt gatgacgtga tcggtctcga actgccaggc ctccgagtcg
     2041 agcatggcga acgccgagga ggcgcactgc acgtccttgg gcaaaaagcc ctcgccgatc
     2101 agcttgcccg gctccgccag atagaggatc tgctcgcggc cctcggacga gatcaagctc
     2161 agcttgaatt cgcccgattt gacgacgaac aggccaggac cgggctcacc tcggcgaaac
     2221 accgagcgct tgcgcttgac ctcgatcagt tcggcaccgc gacacaactc gaccatgacc
     2281 tcgtctggaa cctggtccca tccggcgtag cgcaccagcg cctggcgaac ttgctcgatt
     2341 tccgcagcct cgatcggcat ggcgcgaagt gtaaccggtt gcgccgccgg ggggtagcaa
     2401 gctcacacga ctgcggttgg aaagcttcgc gtgcgtcaag tgcgcatgca aatcgcgcag
     2461 ttctttgctt tgcctacatg tgctctctac actgacgagg tcacaccccg cccggagcct
     2521 tcgatgcaga cccaaaccgc caagccgccc cttcgcctgc cccccgcgcc ccgccccggt
     2581 ggtgcccccg tgttcgagcg ccggcgcaac cggccc
//
LOCUS       DASWNJ010000144         6012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_462135, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000144 DASWNJ010000000
VERSION     DASWNJ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462135"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1258
                     /locus_tag="VGB85_02670"
     CDS             <1..1258
                     /locus_tag="VGB85_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132947.1"
                     /translation="RLAPLAVAALLGCGADGQDAVGIVDDASTSDTTAADTTAADTTA
                     ADDPTAALDAPAVDPHDRIATECEQRFSCGCPSDRFADIAACEEYYRVDWIGAVAKIE
                     AAGLTPDFACYMRWMPYESYGCDSQTEYAEHYEPHACSFCQYGHGDRQVGEPCQDFGE
                     AIQNCAQGLLCSPSAQGDARCIDPCSQAGAGESCNYLPCADGLYCEWADQTCHPGGGE
                     GEPCTALPCDDGLICHPQTSICTPGAGLGEACGSEPCALDLYCDGQVCATPAGPGEDC
                     TVAGCEPGLICRSDDSTCAAPAQAGESCIGSYCADGLLCDWGLDTCAPPSGVGGTCTD
                     WPCLDGLTCDYQTMTCAGPPGLGEPCIDAYCANGLLCNYMVEPVVCVAQPVAGEPCVL
                     DTCAASFVCDAKVQVCVDEPAMLCNM"
     gene            complement(1305..1745)
                     /locus_tag="VGB85_02675"
     CDS             complement(1305..1745)
                     /locus_tag="VGB85_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132948.1"
                     /translation="MQRLLVITAAFALAACKPVVAGSGSSAPMAEAYDKPSDFAGEWV
                     GESNGVLGALKIRSLGPGRYYGQFLGDDGQTRFVCNMQQPLVAPVGGVEPLLGNLVRF
                     TWQDGRGGRGDGWVLINPEDSALTGEIRVGNQGHAMDFVRVDEG"
     gene            1929..2729
                     /locus_tag="VGB85_02680"
     CDS             1929..2729
                     /locus_tag="VGB85_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132949.1"
                     /translation="MTGILAVGVGQGPGVALAGPAPAAPAASGTKSGLGSSSPGTTAT
                     APTRPTPSPPDDRGADPPLAPISPEAATAKPLDDPRRTGQDQPSLVAPANNVTPVSTT
                     AGMGGRTISADDPESKRARAELEGTALSSDKAALADVPARLPPLQRGAWWSMFGAFAL
                     ASAGGVFAGLAEVQEDKAERLAITLDSSTGMQLPYADIQGEYNDILRVGRRDAGVAKG
                     FLAGGAGFLVAGVVLFIVHAVRVRKADAAKRTRTARVGLARGGLEVRF"
     gene            2732..3187
                     /locus_tag="VGB85_02685"
     CDS             2732..3187
                     /locus_tag="VGB85_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132950.1"
                     /translation="MTPPTPPTRAWSRGHVLAGLAALLAFGTALGACFNPPAAAVMFS
                     CDPAAATCPDGYTCEADGCCHRDGSDVEADFGGCGLGKGASAGPTTMTTATTGAPDTT
                     DAPTTGTTADTTSGSSSSSASSTGSEPDTGTTGTTSGTSSGSSSAGMTE"
     gene            3215..3958
                     /locus_tag="VGB85_02690"
     CDS             3215..3958
                     /locus_tag="VGB85_02690"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY0132951.1"
                     /translation="MGIKDIFSSSGRSKNRLDRNMKAVVNIYGQSADRYHAMQELLEI
                     GTVPAIVGVMRRFTINASKSIEDEEEKNWLSKRLTDLDKSILLPAAREFCLNNDNIAW
                     VLRIVEERANEREEWELFDALLEKHPPVYERDPSKKQQLLAHIAEVDNPRVAEILTRY
                     LADPDETIRLQAVDALLDIAEPASPDAGLIDALTARLAHKDEDSLRLKTKVLTGLARL
                     KWDVRAQMDRIVPNLGTEHSFDGTVIKER"
     gene            3962..>6012
                     /gene="carB"
                     /locus_tag="VGB85_02695"
     CDS             3962..>6012
                     /gene="carB"
                     /locus_tag="VGB85_02695"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY0132952.1"
                     /translation="MPRRSDLRRICVLGSGPIVIGQASEFDYSGTQAVKALREEGYEV
                     ILINSNPATIMTDPDLAERTYVEPLTPEFLERVLAAEKPDAILPTVGGQTALNLALEA
                     GRQGILERHGVELLGAHLPAIEKAEDRDQFKAAMLKIGLDVPRSVYCHSLAEAKAALE
                     TIGFPAIIRASFTLGGSGAAISYNREEFDAQVEHGLSESMRHEIMVEESVLGWKEYEL
                     EVMRDAKDNFVVVCSIENLDPMGVHTGDSITIAPAQTLTDKEYQRMRDQARMVVTEVG
                     VTTGGCNIQFAVDPKTGRMIVIEMNPRVSRSSALASKATGFAIAKFAALLAVGYTLDE
                     LQNDITRVTPASFEPSIDYVVTKIPRFAFEKFSGTDETLTIQMKSVGEVMAIGRTFRE
                     SLGKAMCSLETGTYGFDVQSPDATAASVEALLGRPHPLRLWHVGAALRLGVSQERVHE
                     LTRIDPWFLGQIAAVIELEQAVATTPVLSLPLLHAAKRSGLADRRLAALRGVAESEIR
                     ALRLASGLRPVYKRIDTCAAEFAAHTPYLYSSYEQEDESETSTRRKVVILGGGPNRIG
                     QGIEFDYCCVHAAFALRDAGIETIMINCNPETVSTDYDTSDRLYFEPLTAEDALEICR
                     VEASRGELLGVIVQLGGQTPLKLAQALEDAGIPILGTSPDAIDLAEDRERFAKLVNKL
                     KL"
BASE COUNT          952 a         2336 c         1915 g          809 t
ORIGIN      
        1 ccgcctcgcg ccgctcgccg tcgccgccct gctcggctgc ggcgcggacg gccaggacgc
       61 ggtcggcatc gtcgatgatg cgtcgacctc cgacaccacg gcggccgaca ccaccgccgc
      121 cgacaccacc gccgccgacg accccaccgc cgccctcgac gctcccgccg tcgacccgca
      181 cgaccggatc gcgaccgagt gcgagcagcg attctcgtgc ggctgcccca gcgaccgctt
      241 cgccgacatt gccgcctgcg aggagtacta ccgggtcgac tggatcggcg ccgtggccaa
      301 gatcgaggcc gcggggctga cccccgactt cgcgtgttac atgcggtgga tgccgtacga
      361 gtcctacggc tgcgactcgc agaccgagta cgccgagcac tacgagccgc acgcgtgctc
      421 gttttgccag tacggacacg gcgaccgcca ggtcggcgag ccgtgccagg acttcggcga
      481 ggcgatccag aactgcgctc agggcctcct gtgttctccg tcggcgcagg gtgacgcgcg
      541 gtgcatcgac ccgtgctcgc aggccggcgc gggcgagagc tgcaactacc tcccgtgcgc
      601 ggacggcctg tactgcgaat gggccgatca gacctgccac cccggcggcg gcgagggcga
      661 gccctgcacc gccctcccgt gcgacgacgg cctgatctgt cacccgcaga cctccatctg
      721 cacgccgggc gcagggctcg gcgaggcctg cgggagcgag ccgtgcgcgc tcgacctgta
      781 ctgcgacggt caggtgtgcg ccacgccggc gggcccgggc gaggactgca cggtcgcggg
      841 atgcgagccg gggctcatct gccgctcgga cgactcgacc tgcgccgccc cggcccaggc
      901 cggcgagagc tgcatcggga gctattgcgc ggacggactg ctctgcgact gggggctcga
      961 cacctgcgcg cccccgagcg gggtcggcgg cacctgcacc gactggccgt gcctcgacgg
     1021 gctgacgtgc gactaccaga cgatgacctg cgcgggaccc ccggggctcg gcgagccctg
     1081 catcgacgcc tactgcgcga acgggctcct gtgcaactac atggtcgaac cggtggtgtg
     1141 tgtcgcccag cccgtcgccg gcgagccatg cgtgctcgac acctgcgccg cgagcttcgt
     1201 gtgcgacgcg aaggtccagg tctgcgtcga cgagccggcg atgctctgca acatgtgaac
     1261 cgcgctcctc gccgcgctcc ggcgatcctc gctgcggcgg gcgcctagcc ctcgtcgacg
     1321 cgcacgaagt ccatcgcgtg gccctgattt ccgacccgga tctcgcccgt cagcgccgag
     1381 tcctcggggt tgatcagcac ccagccgtcg ccccgtccgc cgcgcccgtc ctgccacgtg
     1441 aagcgcacga ggttgccgag cagcggctcc accccgccga ccggcgcgac gagtggttgc
     1501 tgcatgttgc agacaaagcg cgtctgtccg tcgtcgccga ggaactgacc gtagtagcgc
     1561 ccgggcccga gcgaccggat cttgagcgcg ccgagcacac cgttgctctc gccgacccac
     1621 tcgcccgcga agtcgctcgg cttgtcgtac gcctcggcca tcggcgcgga cgaaccactg
     1681 ccagccacga ccggcttgca cgcggccagg gcgaaggccg ccgtgatgac gagcaggcgc
     1741 tgcacgggac gcagtggata ccatccccca ggagaccgcc agcggtggca aaagtcacgc
     1801 cttggcctcg ccaggaccgc gaacgcacgc acccacgcct ttgcgcggcc gcccccgccg
     1861 tctacaatcc cgccccgacc atgccccggg cttcgtcgcc aacacgcgct ctcggcattc
     1921 tgttacgggt gaccggcatc ctggctgtcg gagtcgggca aggcccgggc gtcgccctcg
     1981 ccgggcccgc gcctgcagcg cccgcggcct cgggcaccaa gtccggtctg gggtcgtcga
     2041 gcccgggcac gaccgcgacc gcaccaacgc ggccgacgcc gagcccgccc gacgatcgcg
     2101 gggccgatcc cccgctcgcg ccgatcagcc ccgaggccgc caccgccaag ccgctcgacg
     2161 acccgcgccg caccggccag gaccagccct cgctggtcgc cccggccaac aacgtcacgc
     2221 cggtgtcgac gaccgccggc atgggcgggc gcacgatctc cgccgacgac cccgagtcca
     2281 agcgcgcccg cgccgagctc gagggcaccg cgctgtcctc cgacaaggcc gcgctcgccg
     2341 acgtgcccgc tcgcctgccg ccgctgcaac gcggcgcgtg gtggtcgatg ttcggggcgt
     2401 tcgcccttgc cagcgccgga ggtgtgttcg ccggcctcgc cgaggtccag gaggacaagg
     2461 ccgagcgcct ggcgatcacc ctcgacagca gcaccggcat gcagctgccc tacgccgaca
     2521 tccagggcga atacaacgac atcctccgcg tcggccgccg cgacgccggc gtcgccaagg
     2581 gcttcctcgc cggcggcgcc ggcttcctcg tcgccggcgt cgtcctcttc atcgtccacg
     2641 ccgtgcgcgt ccgcaaggcc gacgccgcca agcgcacccg caccgcccgc gtcggcctcg
     2701 cccgcggcgg cctcgaggtc cggttctagc gatgactccg cccaccccac cgacgcgagc
     2761 ctggtctcgc ggacatgtcc tcgcggggct cgccgcgctc ctggccttcg ggacagctct
     2821 cggcgcctgc ttcaacccgc ccgccgccgc cgtcatgttc tcgtgcgacc ccgcggccgc
     2881 gacctgcccg gacggctaca cctgcgaggc cgacggctgc tgccaccgcg acggcagcga
     2941 cgtcgaggcc gacttcggcg gctgcggcct cggcaagggc gccagcgccg gcccgaccac
     3001 catgaccacc gccaccaccg gcgccccgga caccacggac gcgccgacca ccggcaccac
     3061 cgccgacacg acctccggtt catccagttc ttccgcttca tcgaccggca gcgagccgga
     3121 caccggcacc accggcacca ccagcggcac ttcgagcgga tcttcgagcg cgggcatgac
     3181 cgaataggtc ggcgcgccac attgagggag cacagtggga atcaaggaca tcttcagcag
     3241 cagcggccgc agcaagaacc ggctcgaccg gaacatgaag gcggtcgtca acatctacgg
     3301 ccagtccgcc gaccgctacc acgcgatgca ggagctgctg gagatcggca cggtcccggc
     3361 gatcgtcggc gtcatgcggc gcttcacgat caacgccagc aagagcatcg aggacgagga
     3421 ggagaagaac tggctgtcca agcgcctgac cgacctcgac aagtcgatcc tgctgccggc
     3481 cgcgcgcgag ttctgcctca acaacgacaa catcgcctgg gtcctgcgca tcgtcgagga
     3541 gcgggccaac gagcgcgagg agtgggagct gttcgacgcc ctcctcgaga aacacccgcc
     3601 cgtctacgag cgcgacccga gcaagaagca gcagctcctc gcgcacatcg ccgaggtcga
     3661 caatccccgc gtcgccgaga tcctgacccg gtacctcgcc gacccggacg agacgatccg
     3721 gctgcaggcc gtcgacgccc tgctcgacat cgccgagccc gccagccccg acgccggcct
     3781 gatcgacgcc ctcaccgcgc gcctcgccca caaggacgag gactcgctgc gcctcaagac
     3841 caaggtcctc accggcctcg cgcgcctcaa gtgggacgtg cgcgcgcaga tggaccgcat
     3901 cgtgcccaac ctcggcaccg aacacagctt cgacggcacc gtcatcaagg agcgctagaa
     3961 catgccccgc cgcagcgacc tccgccggat ctgcgtcctc ggctcgggcc cgatcgtcat
     4021 cggtcaggcc agcgagttcg actactcggg cacccaggcc gtcaaggcgc tgcgcgagga
     4081 gggctatgag gtcatcctca tcaactccaa tcccgcgacg atcatgaccg acccggacct
     4141 cgccgagcgg acctacgtcg agccgctgac gccggagttc ctcgagcgcg tgctcgcggc
     4201 cgaaaaaccg gacgcgatcc tgccgaccgt cggcggtcag actgccctca acctcgcgct
     4261 cgaggccggc cgccagggca tcctcgagcg ccacggcgtc gagctgctcg gcgcccacct
     4321 gcccgcgatc gagaaggccg aggaccgcga ccagttcaag gccgcgatgc tcaagatcgg
     4381 cctcgatgtc ccgcgcagcg tgtactgcca ctcgctcgcc gaggccaagg ccgcgctcga
     4441 gacgatcggc ttcccggcga tcatccgcgc cagttttact ctcgggggca gcggcgcggc
     4501 gatttcctat aatcgcgagg agttcgacgc ccaggtcgag cacggcctgt ccgagtcgat
     4561 gcgccacgag atcatggtcg aggagtccgt gctcggctgg aaagagtacg agctcgaggt
     4621 catgcgcgac gccaaggaca acttcgtcgt cgtgtgctcg atcgagaacc tcgatccgat
     4681 gggcgtgcac accggcgaca gcatcacgat cgcccccgcc cagacgctga ccgacaagga
     4741 gtaccagcgc atgcgcgacc aggcgcgcat ggtcgtcacc gaggtcggcg tcaccaccgg
     4801 cggctgcaac atccagttcg ccgtcgaccc gaagaccggt cgcatgatcg tgatcgagat
     4861 gaacccccgc gtgtcgcgct cgtcggcgct cgcctccaag gcgaccggct ttgcgatcgc
     4921 caagttcgcc gccctgctcg ccgtcggcta caccctcgac gagctgcaaa acgacatcac
     4981 ccgcgtcacc ccggccagct tcgagccgag catcgactac gtcgtcacca agatcccgcg
     5041 cttcgccttc gagaaattct ccggcaccga cgagaccctg acgatccaga tgaagtccgt
     5101 cggcgaggtc atggcgatcg gccgcacctt ccgcgagtcg ctcggcaagg cgatgtgcag
     5161 cctcgagacc ggcacctacg gcttcgacgt gcagagcccc gacgcgaccg ccgccagcgt
     5221 cgaggcgttg ttgggtcgcc cccacccgct gcgcctgtgg cacgtcggcg ccgccctgcg
     5281 cctcggcgtc tcccaggagc gcgtgcacga gctgacgcgg atcgacccgt ggttcctcgg
     5341 ccagatcgcc gccgtcatcg agctcgagca ggccgtcgct accactccgg ttctcagctt
     5401 accgctgctg cacgccgcca agcgcagcgg cctcgccgac cgccgcctcg cggcacttcg
     5461 tggcgtcgca gagtccgaga tccgcgccct gcgcctcgcc tccggcctgc ggccggtcta
     5521 caagcgcatc gacacctgcg ccgccgagtt cgccgcgcac acgccctacc tctactcgag
     5581 ctacgagcag gaggacgaga gcgagaccag cacgcgccgc aaggtcgtca tcctcggcgg
     5641 cggccccaac cgcatcggtc agggcatcga gttcgactac tgctgcgtcc acgccgcctt
     5701 cgccctgcgc gacgccggca tcgagacgat catgatcaac tgcaaccccg agaccgtctc
     5761 gaccgactac gacacctcgg accggctcta cttcgagccg ctcaccgccg aggacgcgct
     5821 cgagatctgc cgggtcgagg cgtcccgggg cgagctcctg ggcgtgatcg tccagctcgg
     5881 cggtcagacc ccgctcaagc tggcccaggc gctggaggat gccggtatcc cgattctcgg
     5941 cacctcgcct gacgcgatcg accttgccga agaccgcgaa cgctttgcca agctggtcaa
     6001 caagctgaag ct
//
LOCUS       DASWNJ010000145         2616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_426711, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000145 DASWNJ010000000
VERSION     DASWNJ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426711"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VGB85_02700"
     CDS             complement(<1..492)
                     /locus_tag="VGB85_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132953.1"
                     /translation="MPYVPPETVQAGPGAPRLHIELTRLADLKLYDGTTSSPVCAAPC
                     DRVIDGRRGQLFFFNGDHVLKSRKFSLTEHSGDVVARVKPGRPARRVGGIIGLALGGA
                     GFGTAAFLYWSARRHADGLNEFREDTDPQAEPNYKPATALLITGAIVAAGGLAMLLLS
                     GTRV"
     gene            complement(807..1724)
                     /locus_tag="VGB85_02705"
     CDS             complement(807..1724)
                     /locus_tag="VGB85_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132954.1"
                     /translation="MPTKSRRCYAFAVLVLPLAFGGLPVQARAFAPGPLPNPTAAATA
                     ERDVVYFQNGGMVLGQIVEILPGDSLTMVTTAGDRKRFTWAELASYERNGVKTEVPAE
                     PAVPAAAADRVDPPEPIQLRSGAPRLHIELTRPVDLKLYEVTGEFSGFNYRGFSTGTT
                     SRPVCVAPCDQVVDGHQGQSFFFNGDGVVKSRDFSLSDYGGDVVVRVKPGSSGLRVGG
                     IVVMSLGGAGLAGGTFLLLTAKNGIDQEYDDMNRPLPLAQPNYVPATALLISGALVVA
                     GGLAMLLLSRTKVHFSQRTAAAKRRLGLG"
     gene            complement(1771..>2616)
                     /locus_tag="VGB85_02710"
     CDS             complement(1771..>2616)
                     /locus_tag="VGB85_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132955.1"
                     /translation="APAEPPPPSEPVAPAESATYNLRGTPMKGRTRHLEVNLDVMDAQ
                     LAFKVGPIVVPGTMTLQVRCIDDLEILDVSDGMVRQGRLTHVVDKTTTTMQMSIPGGE
                     AETESEEEFGELHGRTELIELNDGQWTRTLVGAAPSANLVAELQAAPTEDALYPVAIK
                     IGESWTVPGSELRHWTGSDFTLARGQLKRTLRAVEVVSAETIATIDGVGELGGKIRDG
                     DGKEMDMTMTIQVTERRSLERAMEIEMIGKGTIQLSGTFLEDGLPVSLSVTGPLTLQI
                     KGSLL"
BASE COUNT          442 a          834 c          906 g          434 t
ORIGIN      
        1 aacgcgtgtg ccgctgagca ggagcatggc gaggcccccc gccgcaacga tcgcgccggt
       61 gatcaacagc gctgtcgcgg gcttgtagtt cggttcggcc tgcgggtccg tgtcctccct
      121 gaactcgttg agtccatcgg cgtggcgcct ggcactccaa tatagaaatg ccgccgtacc
      181 gaagccggca cctccgagcg ccaggccgat gatgccgccc acgcgccgcg ccgggcgacc
      241 gggcttgacc cgcgcgacga catcgccgct gtgctcggtg agcgagaact tcctcgactt
      301 gaggacgtgg tcgccgttga aaaagaacaa ctgcccccgg cgcccgtcga tgacccgatc
      361 gcagggggcc gcgcagaccg ggctcgacgt cgtgccatcg tacagcttca gatcggccag
      421 gcgggtcagc tcgatgtgca ggcgcggcgc gccggggccc gcctggaccg tctccggcgg
      481 gacgtatggc acggggacga cggccggcgg gctcgctgct gccgccgcgg ggggaccccg
      541 tagcgggccc ggcgggaggg cgtgagcctg gacaggaagc ccgccgaagg cgagcgggag
      601 cacgagaacg gcaaatgcgt agcaacgccg tgacttggtc ggcatgaacg aatctcctga
      661 atgtttgggg gggttggggg gcggactaat cgctaagttt ggaggtgagt cgctcgccct
      721 tggcaatctt ggtccgggcg gcgatcccag cgacgcacag gccgagctgg cctcgtgctg
      781 gccgaatagg atcaaggtct tgtcggtcag ccgaggccga ggcgacgctt ggctgccgcc
      841 gtgcgctgtg agaagtggac cttggtgcgg ctgagcagca gcatggcgag gccgccagcc
      901 acgacgagcg cgcccgagat cagcagcgcc gtcgcaggca cgtagttcgg ctgcgcgagc
      961 ggcagcggcc ggttcatgtc gtcgtactcc tggtcaatgc cgttcttggc ggtcagcaac
     1021 aagaacgtcc cgccagcgag gcccgcgccg ccgagcgaca tgacgacgat gccgccgaca
     1081 cgcagccccg aactacctgg tttgacgcgg actaccacgt cgccaccgta gtcggagagc
     1141 gagaaatccc gcgacttgac gacgccgtcg ccgttgaaaa agaacgactg gccttgatgg
     1201 ccgtccacga cctggtcgca cggcgcgacg cagaccggcc ttgatgtggt gccggtgctg
     1261 aatccgcggt agttgaaccc cgaaaactcg ccggtgacct cgtacagctt caggtcgacg
     1321 gggcgcgtca gctcgatgtg caggcgcggc gcaccggagc ggagctggat tggctccggc
     1381 gggtcaaccc gatcagccgc cgccgctggc accgctggct ccgccggaac ctcggtcttc
     1441 acgccgttac gctcgtagct ggcgagttca gcccaggtga aacgcttgcg gtcgcccgcc
     1501 gtcgtcacca tggtcagcga gtcacctggc aggatctcga cgatctggcc gagcaccatg
     1561 ccgccgttct ggaagtagac gacgtcgcgc tcggcggtgg cagccgcggt agggttgggt
     1621 agcggacccg gcgcgaaggc tcgggcctgg acaggaagcc cgccgaaggc gagcgggagc
     1681 acgagaacgg caaatgcgta gcagcgccgt gacttggtcg gcataaaaag actcctgaat
     1741 gggggggggg ggaaacgcag cgagctgagg tcagaggagt gatcccttga tctgaagcgt
     1801 gagggggcct gttacggaaa gcgacacggg cagcccatcc tcgaggaagg tcccggacag
     1861 ttggatcgtg cccttgccga tcatctcgat ctccatggca cgctccaggg agcggcgttc
     1921 cgtcacctgg atggtcatcg tcatgtccat ctccttgcca tctccgtcgc ggatttttcc
     1981 gccgagctcg ccgacgccat cgatcgtggc gatggtctcg gctgacacga cctcgaccgc
     2041 gcgaagcgtg cgcttgagct ggcccctggc aagcgtaaag tcgcttcccg tccagtgccg
     2101 aagttccgac ccgggcaccg tccacgactc cccgatcttg atcgctaccg ggtagagcgc
     2161 gtcctcggtg ggtgcggcct ggagttcggc aacaagattg gcggatggcg cggcaccaac
     2221 gagcgtgcgc gtccactgcc cgtcattgag ctcgatcagt tcggtgcgac cgtgcaactc
     2281 gccgaactcc tcctcggact ccgtttcggc ttcgccacct ggaatggaca tttgcatggt
     2341 agtcgtcgtc ttgtcgacga catgggtcaa acggccctgg cgaaccattc cgtcgctcac
     2401 gtccaggatc tcgaggtcat cgatacatcg gacctggagg gtcatcgtgc ctggcacgac
     2461 gatcggtccg accttgaaag cgagctgcgc gtccatgacg tcgaggttga cctcgagatg
     2521 acgggtccga cctttcatcg gcgtgccgcg aaggttgtaa gtggctgact cggccggtgc
     2581 taccggctcg ctcggcggcg gcggctcggc gggcgc
//
LOCUS       DASWNJ010000146         5060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_3054, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000146 DASWNJ010000000
VERSION     DASWNJ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3054"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VGB85_02715"
     CDS             complement(<1..473)
                     /locus_tag="VGB85_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132956.1"
                     /translation="MLALLGGVSLALAQVPAPPAVELRWDAPPGCPGQAALEQEVAAL
                     LADHEPPPRATPARLHFRVERRGATWHLHGEITSSELSGHRRLSATTCEELLAAAALL
                     TAIVVDPAGLDEPGEPEPAPVEPEVVPIVPAHEPPRPVTPEIRAPRPSPPREPPAR"
     gene            575..853
                     /locus_tag="VGB85_02720"
     CDS             575..853
                     /locus_tag="VGB85_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132957.1"
                     /translation="MTLLAALLYLVNCLVGVAAQLTGVGFGRWHHALYALVFATSATA
                     LVVDFHPGLCVTVAALAMFPRARPRTPWHPLLAVLGLLGHLVTLLSRA"
     gene            884..1684
                     /locus_tag="VGB85_02725"
     CDS             884..1684
                     /locus_tag="VGB85_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY0132958.1"
                     /translation="MDLRDAILRRRTTNGPFKPDPVSLEHQHRLMEAASRAPSHFNSQ
                     PWRFVLITDDSIRAKISEIAGQTMESLMSGGRFFNRYSRYFRFSEKEMQTRRDGIFID
                     QMPMAVRPFIKQVFSEAGQKVMNALGVPKTLGKDNAKLVAGSPLLLAALLTREEYIPG
                     ELSGFYSLLGIGMAIENIWLTTADLGMGIQFVSTPMEYPEAWQQIRGLLAVPDDLELI
                     AVYRLGYLPEDKKRPSIDWVSNHRKRLEQYVFRNTCKTPEPDPEERGR"
     gene            1681..2742
                     /locus_tag="VGB85_02730"
     CDS             1681..2742
                     /locus_tag="VGB85_02730"
                     /inference="COORDINATES: protein motif:HMM:NF019988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEY0132959.1"
                     /translation="MTRTVPAVLAVATASPAHRIAQHELRGFARRFFAGNFPKIDRLL
                     PAFDNSGIGERQMASPIEWFDAPHTFAEKNAVYCKCALELAREAGTQALVKAGVTADE
                     VAALVFVSTTGIATPSLDSYLVQQLDLSRATARVPLWGLGCAGGAAGLARAADLTRAL
                     GRPVLLIASEVCSATFVHGDRSKSNLIATALFGDGAAACVIAPVAEDDDAGLRVLGSH
                     THLIDDSDAVMGWTLEAEGLRVRFARSIPDIVRTTVPGFHRATADAAGIDVAEVEHYV
                     LHPGGARVLAAYEAALAVPRERIETAYQVLRAHGNMSSPTVLFVLERFMRDTPRTGAA
                     GLLLGLGPGFAAEGVVFRW"
     gene            2736..3278
                     /locus_tag="VGB85_02735"
     CDS             2736..3278
                     /locus_tag="VGB85_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY0132960.1"
                     /translation="MVSAQTLYWGLLGVVIVQRAAELRLARRNEARAREQGAQEFGAR
                     HYPLFFVIHGTWLLMWFLEAFRRGPHLACGWEFWLGVFIAAEALRYWSIATLGSAWNT
                     RILVVPGAAPVARGPYRWLRHPNYVAVVLELAALPLVFGAWHTALVIGLANLVLLLGV
                     RIPTEMRALQWAGSVAPRSQ"
     gene            complement(3282..4169)
                     /locus_tag="VGB85_02740"
     CDS             complement(3282..4169)
                     /locus_tag="VGB85_02740"
                     /inference="COORDINATES: protein motif:HMM:NF021529.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2259 domain-containing protein"
                     /protein_id="HEY0132961.1"
                     /translation="MSPRKLALTILAATACGAPVSCGAPVVQPLDEAKRPEPPRKDTK
                     DSKTADPATPAAADPAAPATPEPEKSTQPYTRAGDPKDQRDELRFWGFSEDGTKYAFE
                     TYYHGAGGAQCEGQASLFIVDADSDSFVEDSPLVIKHKQPDAERCDPPDLRAEMDRHR
                     EQLLHRHAIVVGNQGAPIVPAPAVDEKGKARASTIALPSGTLRAEVEVIDGDREKVGE
                     RGAAYKLTLVREGQPPKLIEPGTRRRPFVWDYNLDEGVVFVAPDARHLAIVLGTAQRS
                     FEGDRHSYMSNGVKLPDGW"
     gene            complement(4216..>5060)
                     /locus_tag="VGB85_02745"
     CDS             complement(4216..>5060)
                     /locus_tag="VGB85_02745"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEY0132962.1"
                     /translation="THLQDATPLTLGQEFSGYVQQLDHGLAAVEAALPRVYELALGGT
                     AVGTGLNTHRDYAVTSARHIATLTGLPFVTAPNKFAALAGHEPLVGLHGALKVLACAL
                     MKIANDVRWLASGPRCGLGEITIPENEPGSSIMPGKVNPTQCEAMTMVCAQVLGNDVA
                     VGIGASMGNFELNVFKPLIIHNVLHSLRLLGDACRNFREFCAEGIEANLPRIEHNLRE
                     SLMLVTALNPHIGYDNAAKIAKKAHHDGTSLKTAAVALGLLTEAQFDAWVRPETMVGP
                     DAGK"
BASE COUNT          653 a         1685 c         1922 g          800 t
ORIGIN      
        1 cgcgcgggtg gctcgcgcgg cgggctcggc cggggcgcac ggatctcggg cgtcacgggc
       61 cgcggtggtt cgtgggcggg cacgatcggc acgacctcgg gctcgacggg cgcgggctcg
      121 ggttcgccgg gctcgtcgag cccggctgga tcgacgacga tcgcggtcag cagcgcggcc
      181 gcggcgagca gctcctcgca ggtcgtggcg ctgaggcggc ggtgaccgct gagctcggac
      241 gaggtgatct ccccgtgcag gtgccaggtg gctccacggc gctcgacgcg aaagtgcagg
      301 cgcgccggcg tggcccgcgg gggcggctcg tgatcggcga gcagggcggc gacctcctgc
      361 tcgagcgcgg cctgtccggg gcagccaggc ggcgcgtccc agcgtagctc gacggccggc
      421 ggtgccggga cctgcgccag cgcgagcgag acaccgccga ggagcgcgag catgccgggc
      481 gcagcctaca ccacctgccg ccgggcgcat ccttcctgtc ccttcagaca ggtcaccacg
      541 ggcctcgcgc gagggtgtta ggctcacgcg atcgatgacg ctgctcgccg cgctcctgta
      601 cctggtcaat tgtctcgtcg gcgtcgccgc gcagctcacc ggcgtcggct tcggtcgctg
      661 gcaccacgcg ctctacgcac tggtatttgc gacctcggcg acggcgctgg tcgtcgattt
      721 tcatcctggc ctgtgcgtca cggtggcggc gctggcgatg ttcccccgcg cgcggccgcg
      781 aacgccgtgg cacccgttac ttgcggtgct cggcctgctg ggccatcttg tcacgctgct
      841 ctcgcgtgca tgagcaagcg tcgttcgagc acgagccccg accatggacc ttcgagacgc
      901 gatccttcgc cgccggacca ccaacggccc gttcaagccc gacccggtgt cgctcgagca
      961 ccagcaccgg ctgatggagg cggcgtcgcg ggccccgagc cacttcaact cgcagccgtg
     1021 gcgcttcgtg ctgatcaccg atgactcgat ccgcgcgaag atctcggaga tcgccggcca
     1081 gacgatggag tcgctgatgt ccggcgggcg gttcttcaac cgctacagcc gctacttccg
     1141 gtttagcgag aaggagatgc agacgcggcg cgacggcatc ttcatcgacc agatgccgat
     1201 ggcggtgcga ccttttatta aacaggtgtt ctcggaggcc ggccagaagg tgatgaacgc
     1261 gctcggggtc ccgaagacgc tcggcaagga caacgccaag ctggtcgccg gcagcccgct
     1321 gttgctcgcc gcgctgctga cccgcgagga gtacatcccg ggcgagctga gcgggtttta
     1381 cagcctgctc ggcatcggca tggcgatcga gaacatctgg ttgacgaccg cggacctcgg
     1441 catggggatc cagttcgtgt cgacgccgat ggagtacccg gaggcctggc agcagatccg
     1501 cgggttgctg gcggtgccgg acgacctcga gctgatcgcg gtgtatcgcc tcggctacct
     1561 gccggaggac aagaagcggc cgagcatcga ctgggtcagc aatcaccgca agcggctcga
     1621 gcagtacgtg tttcgcaaca cgtgcaagac gccggagccc gaccccgagg agcgcgggcg
     1681 atgacccgca cggtcccggc ggtgctcgcg gtcgcgaccg cctcgccggc gcaccggatc
     1741 gcccagcacg agctgcgagg cttcgcgcgc cggttcttcg ccggcaactt cccgaagatc
     1801 gaccgcctgc tgccggcgtt cgacaacagc gggatcggcg agcggcagat ggcgagcccg
     1861 atcgagtggt tcgacgcgcc gcacaccttc gccgagaaga acgcggtgta ctgcaagtgc
     1921 gcgctcgagc tggcccgcga ggccgggacg caggcgctcg tgaaggccgg cgtcaccgcg
     1981 gacgaggtcg cggcgctggt gttcgtgtcg accacgggga tcgcgacgcc gagcctcgac
     2041 agctacctcg tgcagcagct cgacctctcg cgcgcgacgg cccgcgtgcc gctgtggggt
     2101 ctcggctgcg ccggtggggc ggccggcctg gcccgcgccg ccgacctgac gcgcgcgctc
     2161 gggcgtccgg tgctgctgat cgcctccgag gtgtgcagcg cgaccttcgt gcacggggac
     2221 cggagcaagt cgaacctgat cgccacggcg ctgttcggcg acggcgcggc ggcgtgcgtg
     2281 atcgcgccgg tggccgagga cgatgacgcc gggctgcgcg tgctcggcag ccacacgcac
     2341 ctgatcgacg acagcgacgc ggtgatgggc tggacgctcg aggccgaggg gctgcgcgtc
     2401 cgcttcgccc gcagcatccc cgacatcgtg cgcacgacgg tgccgggctt tcaccgcgcg
     2461 accgccgatg cggccgggat cgacgtcgcg gaggtcgaac actacgtgct gcaccccggc
     2521 ggcgcgcggg tgctcgccgc ctacgaggcc gcgctcgcgg tgccgcgcga gcgcatcgag
     2581 accgcgtacc aggtgctgcg cgcgcacggc aacatgtcga gcccgacggt gctgttcgtg
     2641 ctcgagcgct tcatgcgaga cacgccgcgc accggcgcgg cggggctgct gctcggcctc
     2701 gggcccgggt tcgcggcgga aggcgtggtg tttcgatggt gagcgcgcag acgctgtact
     2761 gggggttgct cggcgtggtg atcgtgcagc gggcggcgga gctgcggctc gcgcggcgca
     2821 acgaggctcg cgcgcgtgag cagggcgcgc aggagttcgg cgcgcgccac tatccgctgt
     2881 tcttcgtcat ccatggcacg tggctgctga tgtggttttt ggaggcgttt cgccgcgggc
     2941 cgcacctggc gtgcgggtgg gaattctggc tcggggtgtt catcgcggcc gaggccctgc
     3001 gctattggtc catcgccacg ctcgggtcgg cctggaacac gcggatcctc gtcgtgcccg
     3061 gggccgcgcc ggtcgcccgc gggccgtatc gctggctgcg gcacccgaac tacgtcgcgg
     3121 tggtgctcga gctcgccgcg ctgccgctgg tgttcggggc ctggcacacg gcgctggtga
     3181 tcggcctcgc caacctcgtg ctgctgctcg gcgtgcgcat cccgaccgag atgcgcgcgc
     3241 tgcagtgggc cggctcggtc gcgccgcgat cgcagtgacg atcaccagcc gtcgggcagc
     3301 ttgacgccgt tgctcatgta gctgtggcgg tcgccctcga aggagcgctg cgcggtgccg
     3361 aggacgatcg ccaggtggcg ggcgtcgggc gcgacgaaca cgacgccctc gtcgaggttg
     3421 tagtcccaca cgaagggccg gcggcgcgtg ccgggctcga tgagcttcgg cggctggccc
     3481 tcgcgcacga gggtgagctt gtacgccgcg ccgcgctcgc cgaccttctc gcggtcgccg
     3541 tcgatcacct cgacctcggc gcgcagcgtt ccagacggga gggcgatcgt cgaggcgcgc
     3601 gccttgccct tctcgtcgac cgcgggggcc ggcacgatcg gcgcgccctg attaccgacg
     3661 acgatcgcgt ggcggtgcag cagctgctcg cggtgacggt ccatctcggc gcgcaggtcg
     3721 ggcgggtcgc agcgctcggc gtccggctgc ttgtgcttga tgacgagcgg gctgtcctct
     3781 acaaaactgt cgctgtcggc gtcgacgatg aagaggctcg cctggccctc gcactgagct
     3841 ccgccggcgc cgtggtagta ggtctcgaac gcgtacttcg tgccgtcctc cgagaagccc
     3901 caaaaccgca gctcgtcgcg ctggtccttg gggtcacccg ctcgtgtgta gggctgcgtc
     3961 gacttctcgg gctcgggcgt cgcgggggcg gcaggatcgg cggcggccgg ggtcgccggg
     4021 tccgcggtct tgctgtcctt ggtgtccttg cgcggcggct cggggcgctt ggcctcgtcg
     4081 agcggctgga cgaccggagc gccgcaggaa acgggagcgc cgcaggcggt cgcggcgagg
     4141 atcgtgaggg cgagctttcg gggcgacatc gccgggcacg ctagcaactc tggcgggccg
     4201 cggcgagttt gcggctcact tgccggcgtc gggcccgacc atggtctcgg ggcggaccca
     4261 ggcgtcgaac tgggcctcgg tgagcaggcc gagcgcgacc gcggcggtct tgagcgaggt
     4321 gccgtcgtgg tgggccttct tggcgatctt ggcggcgttg tcgtagccga tgtgcgggtt
     4381 gagggcggtg acgagcatca ggctctcgcg caggttgtgc tcgatgcggg ggaggttggc
     4441 ctcgatgccc tcggcgcaga actcgcggaa gttgcggcag gcgtcgccga gcaggcgcag
     4501 cgagtgcagc acgttgtgga tgatcagcgg cttgaacacg ttgagctcga agttccccat
     4561 cgacgcgccg atcccgacgg cgacgtcgtt gccgagcacc tgcgcgcaca ccatcgtcat
     4621 cgcctcgcac tgggtcgggt tgaccttgcc gggcatgatc gaggagccgg gctcgttctc
     4681 cgggatcgtg atctcgccga ggccgcagcg cggacccgag gccagccagc gcacgtcgtt
     4741 ggcgatcttc atcagcgcgc aggcgagcac cttgagcgcg ccatgtaggc cgacgagcgg
     4801 ctcgtgaccg gcgagcgcgg cgaacttgtt gggggcggtg acgaagggca ggccggtcag
     4861 cgtggcgatg tgccgcgccg aggtgacggc gtagtcgcgg tgggtgttga ggccggtgcc
     4921 gacggcggtg ccgccgagcg cgagctcgta gacgcggggc agcgcggcct cgacggcggc
     4981 gaggccatgg tcgagctgct gcacgtagcc ggagaactct tggccgagcg tcagcggggt
     5041 ggcgtcttgc aggtgggtgc
//
LOCUS       DASWNJ010000147         3754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_568144, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000147 DASWNJ010000000
VERSION     DASWNJ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568144"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..1240
                     /locus_tag="VGB85_02750"
     CDS             218..1240
                     /locus_tag="VGB85_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0132963.1"
                     /translation="MRDDDDDDGRPAAPRPAYASRATRVISTQPAQKASFAATFKAIF
                     HELRPVFEAFNRDGLLCVAAREGGTIASYVHLPILSAEGAFGVIGRHECCALTLLDEE
                     ISLRHALVRVTRSGSDEVRVRLIDLGSSNTFHTEDNQACKSVMAEGPMFINLLGYQLF
                     LLPTGSLNPSAWTEDADETWSSFPERVYFDQRMPERMVGRNAQQAAQQPRNRRHTYMT
                     LLPAAQHLRARPGAGGDAVGTILLSAESTQIAHRVSHEDLERGVLIGRYERCQLAGAT
                     GSLSRVHLVLLLDGGDVWAVDTASLNGTIVEGQRISAIQLDREIELRLPSGMGMRWVP
                     EHIPPT"
     gene            1271..2728
                     /gene="sthA"
                     /locus_tag="VGB85_02755"
     CDS             1271..2728
                     /gene="sthA"
                     /locus_tag="VGB85_02755"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HEY0132964.1"
                     /translation="MEPVLSPSGSANSPQEFDTQEFDVIVLGSGPGGEGAAMRASKAG
                     KRVAIVEKYRDVGGGCVHWATIPSKALRQAVRSLQEFRRNPLFADCLEGREITFPMLL
                     QSASSVIHGQVDIRRGFYDRNHVRVFHGPARFLDARTVEVEAPGGARPRLRAAERGGF
                     IIATGSRPYRPPDIDFTHPRIFDSDTILGLDHTPNNITIYGAGVVGCEYASIFRGLEV
                     KVNLVNNRDKLLAFLDEEIIDALSYHLRDQGVLIRHNETYDRIEPLADGVVVHLKSGK
                     KIKSDALLWANGRTGNTQDLGLEQLGIKANSRGQIEVDEFYRSREQPHVYAVGDVVGY
                     PSLASAAYDQGRFAAQHLIAGECDERLIKDIPTGIYTNPEVSSVGRTERELTQEGVPY
                     EVGHALFRSLARAQITGYRVGMLKLLFHRESLQILGIHCFGDQAAEIVHIGQAIMTQQ
                     GEANSLRYFINTTFNYPTMAEAYRVAALNGLNRLF"
     gene            3032..>3754
                     /locus_tag="VGB85_02760"
     CDS             3032..>3754
                     /locus_tag="VGB85_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132965.1"
                     /translation="MSEQRDYAVRTAIHAFYSHSQLLIVAEGELPSPEFEIDISQLVT
                     EESGIPTFQLTRRPRPGTENAPLAKTPFRYGEIFSIGRPPENVLVRHAAGFDEVSVEF
                     TRQDLVDIVLSLSAGDVEVSIPDAAHEDDHGPLPPPPPHAPPLDGSGHAHSHHEHDPS
                     GGAQGNHGNHGNHGNHGNHGNEAPVMSRPLTGSEKAGILSDIAVRYRESIGYSNKLSF
                     DEAFADAVKKLPPASVKFPDMLE"
BASE COUNT          602 a         1430 c         1194 g          528 t
ORIGIN      
        1 cctgcgccca gcgccgcgac agcgcgtcca tcgggcgctc gcctgctgtg tccccggggt
       61 cggccaccgc gcccaccata gcagggcctg ccgcggcggc ctgtgcgggg cgtcctgccc
      121 ccgaatgcgc gcgtgcgaac ctgcccgccg ggcgctggtc cggccgggat cacgcgggga
      181 ctcgccctgg aagcgcgggc gggtatcatg aggcgcaatg cgcgacgacg acgacgacga
      241 cggccgcccg gccgctccgc gaccggccta cgccagccgg gccacgcggg tgatcagcac
      301 gcaacctgcc caaaaggcca gcttcgccgc gaccttcaag gcgatcttcc acgagctgcg
      361 tcccgtgttc gaggcgttca accgcgacgg tctgctgtgc gtcgcggccc gcgagggcgg
      421 cacgatcgcc tcgtacgtgc atctgccgat cctcagcgcg gagggtgcct tcggcgtgat
      481 cggccgtcac gagtgctgcg cgctgacgct gttggacgag gagatctcat tgcgccacgc
      541 gctcgtccgc gtgacccgca gcggcagcga cgaggtccgc gtgcgcctga tcgacctcgg
      601 ctcctcgaac acctttcaca ccgaggacaa ccaggcctgc aagtcggtga tggccgaggg
      661 tccgatgttc atcaacctgc tcggctacca gctgttcctg ctgccgaccg ggtcgctcaa
      721 cccgagcgcc tggaccgagg acgccgacga gacctggtcc tcgttccccg agcgcgtcta
      781 cttcgatcag cgcatgcccg agcgcatggt cggccgcaac gcgcagcagg ccgctcagca
      841 gccgcgcaac cgtcggcaca cgtacatgac cctgctgccg gcggcgcagc acctgcgggc
      901 ccgccccggc gccggcggtg acgccgtcgg cacgatcttg ctgtcggcgg agagcaccca
      961 gatcgcccac cgcgtctcgc acgaggacct cgagcgcggc gtgttgatcg gccgctacga
     1021 gcgctgtcag ctcgccggcg cgaccggcag cctctcgcgc gtgcacctcg tgctgttgct
     1081 cgacggcggc gacgtgtggg ccgtcgacac cgcgagcctc aacggcacga tcgtcgaggg
     1141 ccagcggatc tccgcgatcc agctcgaccg ggagatcgag cttcggctac cttccggcat
     1201 gggcatgcgc tgggtccccg agcacatccc accgacctga gccacactcc tcaggccccc
     1261 cagacccccc gtggagcccg ttttgtcccc atccggcagc gccaactcgc ctcaggaatt
     1321 cgacacccag gaattcgacg tcatcgtgct cggcagcggc cccggcggtg agggtgccgc
     1381 gatgcgggcc agcaaggccg gcaagcgcgt agcgatcgtc gagaagtacc gcgacgtcgg
     1441 cggcggctgc gtgcactggg cgacgatccc gagcaaggcg ctgcgccagg ccgtgcgctc
     1501 gctgcaggag tttcgccgca acccgctgtt cgccgactgc ctcgagggcc gcgagatcac
     1561 cttcccgatg ctgctgcaga gcgcgtcctc ggtgatccac ggccaggtcg acatccgtcg
     1621 cggcttctac gaccgcaacc acgtgcgcgt gttccacggg ccggcgcgct tcctcgacgc
     1681 gcgcaccgtc gaggtcgagg ccccgggcgg cgcgcgcccg cggctgcgtg cggccgaacg
     1741 cggcggcttc atcatcgcca ccggctcgcg cccctaccgt ccgccggaca tcgacttcac
     1801 gcacccgcgg atcttcgaca gcgacacgat cctcggcctc gaccacacgc cgaacaacat
     1861 cacgatctac ggcgccggcg tggtcggctg cgagtacgcg agcatctttc gcggcctcga
     1921 ggtcaaggtc aacctcgtca acaaccgcga caaactgctc gccttcctcg acgaggagat
     1981 catcgacgcg ctgagctacc acctgcgcga tcaaggcgtg ctgatccgcc acaacgagac
     2041 ctacgaccgc atcgagccgc tggccgacgg cgtcgtcgtg cacctcaagt ccggcaagaa
     2101 gatcaagtcg gacgcgctgc tgtgggccaa cgggcgcacc ggcaacacgc aagacctcgg
     2161 cctcgagcag ctcgggatca aggccaacag ccgcggccag atcgaggtcg acgagttcta
     2221 ccgcagccgc gagcagccgc acgtctacgc cgtcggcgac gtcgtcggct atccgagcct
     2281 cgcgtcggcc gcctacgacc agggccgctt cgccgcgcag cacctgatcg ccggcgagtg
     2341 cgacgagcgg ttaattaaag acatcccgac cggcatctac accaaccccg aggtcagctc
     2401 ggtcggccgc accgagcgcg agctgacgca ggagggagtc ccctacgagg tcggccatgc
     2461 gctgtttcgc agcctcgcgc gcgcgcagat caccggctac cgcgtcggca tgctcaagct
     2521 gctgttccac cgcgagagcc tgcagatcct cgggatccac tgcttcggtg atcaggccgc
     2581 cgagatcgtc cacatcggtc aggcgatcat gacccagcag ggcgaggcca acagcctgcg
     2641 ctacttcatc aacaccacct tcaactatcc gaccatggct gaggcctacc gcgtcgccgc
     2701 cctcaacggc ctcaaccgcc tgttttgagc ccgtacagcg ccacttcgtc gtccccctgc
     2761 gcgaccgcga cgcagccccg cggcggtacg gcgttcgcgg cgcctgcgcg acgtggcacc
     2821 gacgcgggcc cgcggcggac ctggcccttg ggacacggga gtcccccgtg ggggccctgt
     2881 cccatggccc ccagggcccc acgacaagcg tcgcagacgc gagcgatccg agtcttcgcc
     2941 gcgctgtgcc gcaggtcgcg cgagatttcg cgcgagcgct ttccagggcg gggtctgtta
     3001 tcctcgccga gccgtcgagg accaggggcc catgtctgag caacgcgact acgccgtacg
     3061 caccgcgatc catgcgttct actctcacag ccagctcttg atcgtcgcgg agggcgagct
     3121 gccctcgccc gagttcgaga tcgacatctc gcagctcgtc accgaggaga gcggcatccc
     3181 gaccttccag ctgacgcgcc gcccccggcc cggcacggag aacgccccgc tcgcgaagac
     3241 accgtttcgc tacggcgaga tcttcagcat cggccgcccg cccgagaacg tgctcgtgcg
     3301 gcacgccgcg ggcttcgacg aggtcagcgt cgagttcacg cgccaggacc tcgtcgacat
     3361 cgtgctcagc ctgtccgcgg gcgacgtcga ggtcagcatc cccgacgccg cgcacgagga
     3421 cgaccacggc ccgctcccgc ccccgccccc gcacgcgccc ccgctcgacg gcagcggcca
     3481 cgcgcacagc caccacgagc acgacccgtc cggcggcgcg cagggcaacc atggcaacca
     3541 cggcaaccac ggcaatcatg gcaaccacgg caacgaggcg cccgtcatgt cgcgtccgct
     3601 gaccggcagc gagaaggccg gcatcctctc ggacatcgcc gtgcgctacc gggagtcgat
     3661 cggctactcg aacaagctct cgttcgacga ggccttcgcc gacgccgtca aaaagctccc
     3721 gcccgcgtcg gtgaagttcc ccgacatgct ggag
//
LOCUS       DASWNJ010000148         4528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_532620, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000148 DASWNJ010000000
VERSION     DASWNJ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532620"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VGB85_02765"
     CDS             <1..771
                     /locus_tag="VGB85_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0132966.1"
                     /translation="VQFTPDLMPTDITGTEILYDDKLTGQRGFRFVHGPIFANIVLAD
                     EINRATPKTQSALLEAMAENQVTAAGKTRPLPEPFLVLATQNPLEMEGTYPLPEAQLD
                     RFLFKIDVPFPGEAELLQILERTTGSRNHVARPVLSVTDLVVIRGFVRAIPVAPTVSR
                     YVVRVLRATHPDDANATDLVKRYVRFGGSPRGAQAILMAARIRSLMAGRKTPSIDDVR
                     TVAPSALRHRVILNFEGEAEGVAVDKVLAQVLTSVRTD"
     gene            781..1749
                     /locus_tag="VGB85_02770"
     CDS             781..1749
                     /locus_tag="VGB85_02770"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY0132967.1"
                     /translation="MAWWKRSDPVTEAPPPRAPAAIARAGEPGDIFDDAFLARLELLQ
                     VIARRLFRGRQRAERKTRKVGSGLEFADHREYSPGDDIRNLDWNVLMRLGQSLIRLYE
                     EDEDLPIRVAIDVSESMQTSGGGKLRYAQQIAAALAYVGLANLDRVGLTCMSRKVHTS
                     LPAVRGKGRIFRVFEFLRAAPKGGATDLRAACSRIAAESVQPGLTVVLSDFYDMDGAF
                     DGLNLLRFRKHEPVAIQILDPAEVHPERSALRGDVSLVDCEGGDPRDVTLSPATLKAY
                     AAAHEQFCAALASNCRARGIPYFRAEIDVPFDELVLRMFRLGGLLR"
     gene            1746..3785
                     /locus_tag="VGB85_02775"
     CDS             1746..3785
                     /locus_tag="VGB85_02775"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0132968.1"
                     /translation="MNLAGWTLAELLPLFLAGSAAITAMYLLRMRRRQLVVPFAALWR
                     QVTRESDTRRLWRKLRRVLSWLLQLLLLALLCLALGDPRPSAWLRDPTTLAIVIDRSA
                     SMAGPAGEAEPGTTRLELALRRARAELSALGPVDRALVITAGPEVAVPGPLGNDPDPL
                     LRSIAELTPQAGEADLTRALALARSALAGQPTGRILVLTDGALDPAGAAALSACAAVP
                     EDMPEGTDAPVRCRVVTFGGPADNVAITAFAARRYPEDREHVEVLAEVRNLGDAPVSV
                     VLEVVADGLNVGRRELALGPGEARTEVLRDLDAARARLTAQLSPGTGTDAAALGPAGD
                     DRAHAIVPPLRPFKVALITDGTDLFLEAALLTLQDHIQLTGVGVADAQNAAVAGADLV
                     VIDVGAEPLPSPLPAGDLVIFDPLRRPENASPIAGAGELSRPFLTEQASGHPLLDGVV
                     LKDVNLGRASKLAALPGDQVLVRSLGDPVALVRREAGQDDSTGARTIVAFGFDPRQSD
                     LPLRTAFPNLVANFVAFFEQSRPGFVASVPIGAARPLAVAELGLVPEGLGNVEVAGPE
                     PADPTRADDLPGIRVPVDRGVFRLRAQEPGIYRVRALDGPDAGAVVLLAVNQASAAAS
                     DLHDRLAAELPSEALAGDPPTPLPVGEGPLWTLLLLAAAGLIALEWATYHRRVTV"
     gene            3817..>4528
                     /locus_tag="VGB85_02780"
     CDS             3817..>4528
                     /locus_tag="VGB85_02780"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEY0132969.1"
                     /translation="MLRPLLALLLGFTAAAALHLWGPQVAPLHVELPAGLASWLSESG
                     DRDLVLTQPAALWLLPLALLPYLVVVARRSLVDLHGWQVALQAVVRLLVLVALSLALA
                     IPTLQSPRRGKTVVLAVDVSASVDDGQLAAAKQLVRDALRTIAGETGDREDRTRLRLL
                     AYATAARAVPIADDPEADPDLSREPAGGLASDHAGALRLAEALLDPDTEGRVILVSDG
                     AGSVAERADLAATARELQA"
BASE COUNT          569 a         1820 c         1517 g          622 t
ORIGIN      
        1 gtgcagttca cccccgacct gatgccgacc gacatcaccg ggacggagat cctgtacgac
       61 gacaagctga ccggccagcg cgggttccgg ttcgtccacg ggccgatctt cgccaacatc
      121 gtgctcgccg acgagatcaa ccgcgcgacc cccaagaccc agtccgcgct gctcgaggcg
      181 atggccgaga accaggtcac cgccgccggc aagacccgcc cgctgcccga gcccttcctc
      241 gtgctggcga cccagaaccc gctcgagatg gagggcacct acccgctgcc cgaggcccag
      301 ctcgaccgct tcctgttcaa gatcgacgtg cccttccccg gcgaggccga gctgctgcag
      361 atcctcgagc gcacgaccgg cagccgcaac cacgtcgccc ggccggtgct gtcggtgacc
      421 gacctcgtcg tcatccgcgg cttcgtgcgt gcgatccccg tcgcgccgac ggtcagccgc
      481 tacgtcgttc gtgtgctgcg tgcgacccac cccgacgacg ccaacgcgac cgacctcgtc
      541 aagcgctacg tccgcttcgg cggcagcccg cgcggcgccc aggcgatcct gatggccgcg
      601 cgcatccgca gcctgatggc cggccgcaag accccgtcga tcgacgacgt gcgaacggtc
      661 gccccctcgg cgctgcgcca ccgcgtgatc ctcaacttcg agggcgaggc cgagggcgtc
      721 gccgtcgaca aggtgctcgc gcaggtgctc accagcgtgc ggaccgactg acccgacccg
      781 atggcgtggt ggaagcgcag cgatccggtc accgaggctc cgcccccgcg ggcgcctgcc
      841 gcgatcgctc gcgccggtga gcccggcgac atctttgatg atgccttcct cgcccgcctc
      901 gagctgctcc aggtgatcgc gcgccggctg tttcgcggcc ggcaacgcgc cgagcgcaag
      961 acgcgaaagg tcggcagcgg cctcgagttc gccgaccacc gcgagtactc gcccggcgac
     1021 gacatccgca acctcgactg gaacgtgctg atgcgcctcg gccagtcgct gatccggctg
     1081 tacgaggagg acgaggacct cccgatccgc gtggcgatcg atgtcagcga gagcatgcag
     1141 accagcggcg gcggcaagct gcgctacgcc cagcagatcg ccgcggcgct cgcctacgtc
     1201 ggcctcgcca acctcgaccg cgtcggtttg acctgcatga gccgcaaggt ccacaccagc
     1261 ctaccggctg tgcgcggcaa agggcggatc ttccgcgtgt tcgagttcct gcgcgcggcc
     1321 cccaagggcg gcgcgaccga cctgcgcgcc gcgtgctcgc ggatcgccgc cgagagcgtg
     1381 cagccgggcc tgaccgtggt gctgagcgac ttctacgaca tggacggcgc gttcgacggg
     1441 ctcaatctcc tgcgctttcg caagcacgag ccggtcgcga tccagatcct cgatccggcc
     1501 gaggtccacc ccgagcgcag cgccctgcgg ggtgatgtca gcctggtcga ctgcgagggc
     1561 ggcgacccgc gcgacgtcac cctcagcccc gcgaccctca aggcctacgc cgccgcgcac
     1621 gagcagttct gcgccgcgct ggcgagcaac tgccgggccc gcgggatccc gtactttcgc
     1681 gccgagatcg acgtgccctt cgacgagctc gtgctccgca tgttccgcct cggagggctg
     1741 ctgcggtgaa cctcgccggc tggaccctcg ccgaattact gccgctgttc ctcgccggca
     1801 gcgccgcgat cacggcgatg tacctgctgc gcatgcgcag gcgccagctc gtcgtcccgt
     1861 tcgcggccct gtggcggcag gtcacccgcg agagcgacac gcgaaggctg tggcgaaagc
     1921 tgcggcgcgt gctgtcgtgg ctgcttcaac tgttgctgct cgcgctgctg tgcctcgcgc
     1981 tcggcgaccc caggccgagc gcgtggctgc gcgacccgac gacgctggcg atcgtcatcg
     2041 accgctcggc gagcatggcg ggtcccgccg gcgaggccga gccgggcacg acacgcctgg
     2101 agctcgcctt gcggcgcgcc cgcgccgagc tgtccgcgct cgggcccgtc gaccgcgcgc
     2161 tcgtcatcac cgcgggcccc gaggtcgccg tgcccggccc gctcggcaac gaccccgacc
     2221 cgctgctgcg cagcatcgcc gagctgacgc cgcaggccgg cgaggccgac ctgacgcggg
     2281 ccctcgcgct cgcccgcagc gccctcgccg ggcaacccac ggggcggatc ctcgtgctca
     2341 ccgacggcgc gctcgacccc gctggcgcgg ccgcgctgtc cgcctgcgcg gctgtcccgg
     2401 aggacatgcc cgaaggtaca gacgcgcccg ttcgctgccg cgtcgtcacc ttcggcggcc
     2461 cggccgacaa cgtagcgatc accgccttcg ccgcgcgccg ctaccccgag gaccgcgagc
     2521 acgtcgaggt cctcgcggag gtgcgcaacc tcggcgacgc gccggtctcg gtcgtgctcg
     2581 aggtcgtcgc cgacggcctc aatgtcggtc gccgcgagct cgcgctcggc cccggcgagg
     2641 cgcgcacgga ggtcctgcgt gacctcgacg ccgcgcgcgc gcgcctgacc gcccagctgt
     2701 ccccggggac aggtacggac gcagccgcgc tcggccccgc gggcgacgac cgggcccacg
     2761 cgatcgtgcc gccgctgcgc cccttcaagg tcgcgctgat caccgatggc accgacctgt
     2821 tcctcgaggc cgcgctgctg accttgcagg atcacatcca attgaccggg gtcggcgtcg
     2881 cggacgcgca aaacgccgcc gtcgccggcg ccgacctcgt cgtgatcgac gtcggcgccg
     2941 agccacttcc gagcccgctg ccggccggcg acctcgtgat cttcgacccg ctgcgccggc
     3001 ccgagaacgc gagcccgatc gccggcgccg gcgagctctc gcggccgttc ctgaccgagc
     3061 aggcgagcgg ccacccgctg ctcgacggcg tcgtgctcaa ggacgtcaac ctcggccgcg
     3121 ccagcaagct cgccgccctc cccggcgacc aggtgctcgt gcgcagcctc ggcgatccgg
     3181 tcgcgctggt ccgccgcgag gccggtcagg acgacagcac cggcgcacgc acgatcgtcg
     3241 ccttcggctt cgacccgcgc cagagcgacc tgccgctgcg caccgcgttc cccaacctcg
     3301 tcgccaactt cgtcgccttc ttcgagcagt cgcgccccgg cttcgtcgcc agcgtcccga
     3361 tcggcgccgc gcgcccgctc gccgtcgccg agctcggcct ggtgcccgag ggcctcggca
     3421 acgtcgaggt cgccggaccc gaaccagctg accccacacg cgcggacgac ctcccgggca
     3481 tccgcgtgcc ggtcgaccgc ggcgtgtttc gcctgcgcgc gcaggagccc ggcatctatc
     3541 gcgtgcgcgc gctcgacggc cccgacgccg gcgccgtcgt gctgctcgcg gtcaaccagg
     3601 cgagcgccgc cgcgtccgac ctgcacgacc gcctcgccgc cgagctgccg tccgaggccc
     3661 tcgccggcga tcctccgacc ccgctgccgg tcggcgaggg cccgctgtgg accctcctgc
     3721 tgctcgccgc cgccggcctg atcgcgctcg agtgggccac ctaccaccgg cgggtgaccg
     3781 tatgaacctg tccctccgga catcttcgga ggcagtgtgc tgaggcccct gctcgcgctg
     3841 ctgctcggct ttaccgcggc cgccgccctg cacctgtggg gcccgcaggt cgccccgctg
     3901 cacgtcgagt tgccggcggg tctcgcaagc tggctgtcgg agtccggcga ccgcgacctc
     3961 gtgctgacgc agccggccgc gctgtggctg ctgccgctcg cgctgctgcc ctacctcgtc
     4021 gtcgtcgccc gccgctcgct cgtcgacctg cacggctggc aggtcgcgct gcaggcggtc
     4081 gtgcgcctgc tggtgctcgt cgccctctcg ctcgcgctgg cgatcccgac cctgcagagc
     4141 ccgcgccgcg gcaagacggt cgtgctcgcg gtcgacgtct ccgcgagcgt cgacgatggc
     4201 cagctcgccg ccgcgaagca gctcgtccgc gacgcgctgc gcacgatcgc tggcgagacc
     4261 ggggatcgcg aggaccgcac ccgcctgcgc ctgctcgcct acgcgacggc cgcgcgcgcc
     4321 gtgccgatcg ccgacgaccc cgaggcggat ccggacctgt cccgcgagcc agccggcggc
     4381 ctcgccagcg atcacgccgg cgccctgcgg ctcgccgagg cgctgctcga ccccgacacc
     4441 gagggtcgcg tgatcctcgt cagcgacggc gctggatccg tcgccgagcg ggccgacctc
     4501 gccgccaccg cgcgtgagct gcaagccc
//
LOCUS       DASWNJ010000149         1572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_355899, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000149 DASWNJ010000000
VERSION     DASWNJ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355899"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..958)
                     /locus_tag="VGB85_02785"
     CDS             complement(32..958)
                     /locus_tag="VGB85_02785"
                     /inference="COORDINATES: protein motif:HMM:NF041117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CBASS oligonucleotide cyclase"
                     /protein_id="HEY0132970.1"
                     /translation="MQTIAQAFETFLPRLELTTAERDNASNQHIYLREQLQQKMAVQD
                     NFLSGSYARHTAVRPLNDIDVFLVLKPTGTLSSATPPDQVLTEVKRVLETIYSGKTAR
                     TQARSVNIEFSGTGIAYDVVPAFSSDDDTYTIPDAEASRWIRTNPRIHAAMSTEANER
                     AGKKLKPLLKAVKQANQRRANPARSFHLEVLAWEVLDYDPGSYIDGLVTLLEGIAARV
                     QRPCPDPARLGGDIRPSSPRCQEAASWFGEMAVLAREARKLAVDGRTGEAHARLREVL
                     GEAWPEKGSVGARAAAVGVMGGSVDDPGSRFG"
     gene            complement(1076..>1572)
                     /locus_tag="VGB85_02790"
     CDS             complement(1076..>1572)
                     /locus_tag="VGB85_02790"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEY0132971.1"
                     /translation="GPYEIRARGATKVAEIMRELDLFKPDILHLHLHGSPTGELGFED
                     ERGETKKIPGEVFVNMLKATGIRPALIVLSACHSAVLASSLLEVAECVIAMTDAVAFK
                     VPITFSVGFYQALGRGHSLASAAEQGKLLAQAEHSTGQEKIEIHSAPGVRADQILLFP
                     QPSR"
BASE COUNT          236 a          521 c          523 g          292 t
ORIGIN      
        1 gcgggacgct tgaaccttcg tattcatggc ctcacccgaa gcggctcccc gggtcatcca
       61 ccgatccacc catcactccg acggccgccg cacgcgcccc gacgctgccc ttctccggcc
      121 acgcctcgcc caacacctcg cgcagcctgg cgtgcgcctc gccggttcgc ccatcgaccg
      181 ccagcttcct cgcttcccgc gcgaggacgg ccatctcccc gaaccagctc gcggcctcct
      241 gacagcgtgg gctcgacgga cggatatcgc cgccgagccg cgcgggatcg gggcagggac
      301 gctggacgcg cgcggcgatc ccttcgagca acgtgaccag gccgtcgatg tagctcccgg
      361 gatcgtagtc caacacctcc cacgcgagga cctcgaggtg aaacgagcgg gccgggttcg
      421 cgcggcgctg gttggcctgc ttgacggcct tgagcagcgg cttcaacttc ttgccggcgc
      481 gttcgttcgc ctccgtcgac atggcggcgt ggatccttgg gttcgtccgg atccatcgcg
      541 atgcctctgc gtccgggatc gtgtatgtgt cgtcgtccga ggagaacgcg gggacgacgt
      601 cgtaggcgat gccggtcccg ctaaactcga tgttgaccga acgtgcctgc gtgcgcgccg
      661 tcttcccgct gtagatcgtc tcgaggacac gcttcacctc ggtgaggacc tgatcgggcg
      721 gagtcgcgct gctcagcgtt ccggtcggct tcaggaccag aaacacgtcg atgtcgttga
      781 gcggccggac cgccgtgtgg cgggcatacg agccgctcaa gaagttgtcc tgaacggcca
      841 ttttctgctg gagctgctcg cggaggtaga tgtgctggtt actggcgttg tcgcgctctg
      901 cggtcgtcag ctccagccgc ggaaggaatg tctcgaatgc ttgggcgatg gtttgcacgg
      961 atgctccggg ggtgaggaca atgagtcgtc ggcgggggcc gatcgatcct cggagcctgc
     1021 tgtccgtgca cactcctact tcggctcaac gagatccgtg aggcctcgca ccacatcagc
     1081 gagagggctg tggaaacagg aggatctggt cggcgcgcac cccgggggcg ctgtggatct
     1141 cgatcttctc ttgtccggtg gagtgctcgg cctgggcgag cagcttgccc tgctcggcgg
     1201 cgctcgccag gctgtgaccg cgtccgaggg cctgatagaa ccccacgctg aaggtgatgg
     1261 ggaccttgaa ggccacggcg tcggtcatgg cgatcacgca ctcggcgacc tcgagcagag
     1321 aggaggccag gaccgcggag tgacacgcgc tgaggacgat tagcgccggc ctgatccccg
     1381 tcgccttcaa catgttgaca aacacctcgc ccgggatctt cttcgtctcg ccccgttcgt
     1441 cctcgaaccc gagctcgccc gtcggggagc cgtgcaggtg tagatgcagg atgtccggct
     1501 tgaacaggtc gagctcgcgc atgatctccg cgaccttggt ggctccacgg gcgcggatct
     1561 cgtatggacc gt
//
LOCUS       DASWNJ010000150        10368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_462288, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000150 DASWNJ010000000
VERSION     DASWNJ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 10368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10368
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462288"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..578)
                     /locus_tag="VGB85_02795"
     CDS             complement(183..578)
                     /locus_tag="VGB85_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132972.1"
                     /translation="MGRRGRLARRPPPTLEIPAASLPILRFEAMRMEGPARIELVGHH
                     DGREGDSPAARDAIARARAQAVHRYIVEVMHHPPAKYDVRLAGPDDADYNDGTATGRA
                     HSRIVELRHDLRCSDVTCQPVLLADTPPP"
     gene            complement(695..2281)
                     /locus_tag="VGB85_02800"
     CDS             complement(695..2281)
                     /locus_tag="VGB85_02800"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III-family protein"
                     /protein_id="HEY0132973.1"
                     /translation="MKPGTWLRTLVHLTPRQVFYQGVRRLRGPARRPVSTTREGLIAV
                     VVPLPPPSAEGVIEPGGVRLLEHPLFDPCADGWEPDADPLYVYTVHYHGWLSRLPLAR
                     ARAQVEHWIDNHLEGVGWEPYPTAMRVLHWLGWLGKHESHLDATARRRIYGSLAAQLE
                     HLRVHVEHHLGGNHVWTDLAALCSAGLSLEGPAAAPLTRHIATFADIVEAQLGPDGVH
                     RERTPTYHCLLAEQLAGVVALNPARVDPAAAARLDAALDRMISALPAFTHPDGDVALW
                     NDSQQGAAVTPRSLFARLDRPPESLLTGPADAPNAGLFRRRFGPWTLLWNAGGIGLPN
                     QPGHIHADGLAFELSLHEERVVIDAGVGTYNPGFARDYARSTRAHSTVTIGDNDSNQH
                     EMWASHRVGGRARTTDLSFGTDHLEARLHGYRAAATHYRRLHWDGRRLVCEDRVEPET
                     TATTRLFFPESCALLLRGSVWHGRTAGGRRFRLVAPAGTPWSCSPAHGWTAMNQSNPR
                     RCLAAPIPPGGLTFEFIAET"
     gene            complement(2278..3540)
                     /gene="wecC"
                     /locus_tag="VGB85_02805"
     CDS             complement(2278..3540)
                     /gene="wecC"
                     /locus_tag="VGB85_02805"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: protein motif:HMM:NF008286.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HEY0132974.1"
                     /translation="MTLSTPAAHETSEYDVCVLGLGYVGLPTAAVLATNGRRVAGVDL
                     RRDVLDTINAGRIHIVEPELDALVRAAVTAGRLRAVSEPVAARNFLICVPTPITPDHQ
                     PDLRHVEAAAHAIVPALRPGCLVVLESTVPPGTLRELVAPIITRGGLVPGRDVFLAHA
                     PERVLPGNVIREVVENERIVGGLTDACTARCAEFYEGFARGQVHRCSAEVAELTKLSE
                     NAFRDVNIAFANELANVCGRLGLDVWEVIRLANRHPRVQILRPGPGVGGHCIAVDPWF
                     IAAAAPLDTPLIRAARHVNDDRPQRVLDQVHQLASRLRRPKIACLGLAYKPDIDDLRE
                     SPALHIAQALAASGEVDLMLVEPHLHRSPIDGVPLVALEQALASADIVVILVAHRQFR
                     HLRPDQLLRLMVVDTVGLLTVGVSGTRA"
     gene            3569..4747
                     /gene="wecB"
                     /locus_tag="VGB85_02810"
     CDS             3569..4747
                     /gene="wecB"
                     /locus_tag="VGB85_02810"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEY0132975.1"
                     /translation="MSSTQPRILIVFGTRPEVIKLWPVDRALRSFPGFQVTLCNTGQH
                     RELVPPLLATFRMVPEVELGVMSPGQSLSSLLARLLDGIDPVLDALRFDWVIVQGDTT
                     TALAAALAAAHRHVPVAHVEAGLRTNDLLRPFPEEGNRQIIARVASLHLAPTATARAA
                     LLREGVPAERIEVTGNTVIDAARAMAEMLPLDGDPPIGSPLELLRFTGDRHVVLVTGH
                     RRESFDGGLASVCEGLAMLARNHPEVDFVYPVHLNPSVQSAVRGTLAGLANVHLLEPL
                     DYPAAIWLLRRCLFVITDSGGLQEEAPEFGKPVLVTRASTERSEGIAAGCAELVGYDR
                     DRLVAAAERFLRDPASARLTIDNPYGDGHAAERCAAALRRCLGLPEDAPRVLDAEDIP
                     "
     gene            4758..6212
                     /locus_tag="VGB85_02815"
     CDS             4758..6212
                     /locus_tag="VGB85_02815"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEY0132976.1"
                     /translation="MSATPWVLGIAGALALLLTALGMPPAVRGWNALGRKDREISLRK
                     VHSGEIPRVGGFVLMLGFAIVAIRGLAGTTELSATHEWFSQNPLTGAVLGALICGAVG
                     LYDDLVGLRARYKLLGQVFAAALAVFGFGLHWEALAALLGPAEILADPLTVLFLVAGV
                     NAINLSDGLDGLAAGNVAIGLSVVVVAAIGHGAAAVPVGWIAATALGAVLGFLIHNRH
                     PARVFMGDAGSYFLGFLLTALLLLVHPVRERVAIIQLSIPLMALALPLFDMTLAIARR
                     AARGQPIFAPDCDHIHHRLLARGLPHARVVQILWLSAALFAGLAHLNVIGVGGWWTLT
                     GAVAAMLVVAVLLGYHNMLRSLPAFTGDHLLGLRDRRRQVMEILAAIDLLAGETSDRE
                     EPRWRRLTPELAPILARLGVPGFEVRRGPEVVARAGTDEGAWAWLSLPLPGSPGAEVR
                     LALAVRLPELQSEQLMLLERVVAVLAGSPLGGAR"
     gene            6252..6728
                     /locus_tag="VGB85_02820"
     CDS             6252..6728
                     /locus_tag="VGB85_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132977.1"
                     /translation="MTPDKFTVVGTFGSVAEAEMARGQLAAAGLEVRLDNAQTLGVLP
                     MHSLAIGGVGVAVATRFAAQARAILGFEEDGRPEEDTEPDSAIALQAPDAWMRRAAVM
                     AVVGSAFILPTLYSVYVLGKYGRGIMSPLGKRNRAIAIVFNAFAFVIVTLFAILSC"
     gene            complement(6730..6936)
                     /locus_tag="VGB85_02825"
     CDS             complement(6730..6936)
                     /locus_tag="VGB85_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132978.1"
                     /translation="MPEPTIISRGRGGVHSRWPGWAVERAAWVHQQVETGYTLPEIAE
                     QVKTGLAPGPTVPEASESTGELGE"
     gene            7225..7929
                     /locus_tag="VGB85_02830"
     CDS             7225..7929
                     /locus_tag="VGB85_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132979.1"
                     /translation="MRGSAGLRALAGGGAQYAVLDAAGAVVTTAAAWDDDAPRAARTR
                     DVLLVHRWLRAAQAGDPEGGLQLGVLRGCAGPWRVCAPVVRVPVLPERECRRGDALRL
                     LEAAPGPALRDRARLRVTGLLACAGLDRPAARSLLRAYEGFFPGVEVGVAWSLHDRLC
                     ARGRSCLHCLDLRSLTWDLLAGASLWTLPETGGGRWPAGVHLFSVHAGAETSPGRRGR
                     RGPASSGPSIPHASPH"
     gene            8062..8259
                     /locus_tag="VGB85_02835"
     CDS             8062..8259
                     /locus_tag="VGB85_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132980.1"
                     /translation="MKWVEIRHDGDGNRGAAYVADVPGGVLVRTVTHLILHTGLYVSE
                     ALAFVPNVTVADFERKPKDED"
     gene            8290..9552
                     /locus_tag="VGB85_02840"
     CDS             8290..9552
                     /locus_tag="VGB85_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HEY0132981.1"
                     /translation="MASQVHDRRRFLAFVLLAAACKPAPAEPARPQAVLVSPEARADA
                     PTWVAGGDAFAIELYQRVRAREGNQFFAPGNLRSALAMTYAGAAGTTAQEMARALHFE
                     SLPGPRLHAAQAAAAADLGTADSGVGGVVLRAANRLWGAQGEPFVPAYLELTRTQYGA
                     ELVGIDFAATEHARQTINAWVSQQTQAKIPELLAAPDIDAATRLVLTSAIYFKGAWLR
                     AFDPAQTLDRPFQVPGLAVPPAVPTMQQKAEFFKYAEPEGVQVLELPYVGDRLAMLIV
                     LPRAADGLPALEAELSPARVAGWVAALRSRKVQVALPRFTTRARFELSGVLAAMGMPT
                     AFTGAANFSEMSRAGGLSISSVVHEAFIDVGEAGTEAAAATAVVMTRSMPIGAPPAFT
                     ADRPFLYFIRDTRSGQLLFVGRVVDPRG"
     gene            complement(9557..>10368)
                     /locus_tag="VGB85_02845"
     CDS             complement(9557..>10368)
                     /locus_tag="VGB85_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132982.1"
                     /translation="IAPGPIGGPLTSKSIAAGVSTRWTGYKEAVRDVTHQLQVMVDRA
                     GFAGRMMLDPIADQATRVLKMWNGRVLLAVGPTKHLRLGLGADDPHAGHRALLTLLRD
                     ITENLQLARMFVSNIPNASLKKVGDNPDIWMLTASGLGNQVPTSLRPLLDDGRLRVAF
                     NGSAHAGGLLIVIGPKADSELKAWLTEAGDAASGKEGMKDLLHATSAASPEAVSAVLQ
                     ALEQNAIDKLFTAVLALAADRAPTQLVMRQVGERYEITVRGPEATPAKPRR"
BASE COUNT         1357 a         3350 c         4095 g         1566 t
ORIGIN      
        1 ccaggggctg ctcgggcgca tcaagttcag gatcccgatc acgggcatcg gcaggagcag
       61 gaccatgacc aggaggcagc gcgagcggtc gacgtgcatg aacctgagcc gcggcctctt
      121 cgacatcacg cgagcttgcc agaatcgtat gtcgccgggc gtatcaagct cgcgcgcaga
      181 cttcagggag gcggggtgtc ggcgagcagc acgggctggc aggtgacgtc gctgcatcgc
      241 aggtcatggc gcagctcgac gatgcgactg tgggcgcgtc cggtggcggt gccgtcgttg
      301 taatcggcgt cgtcggggcc ggcgagccgc acgtcgtact tcgcgggcgg gtgatgcatg
      361 acctcgacga tgtaacgatg caccgcctgc gcgcgggcgc gtgcgatcgc gtcgcgggcc
      421 gcgggcgagt cgccctcgcg gccgtcgtgg tgaccgacga gctcgatgcg cgcggggccc
      481 tccatgcgca tcgcctcgaa gcgcagtatg gggagcgagg cggcggggat ctcgagcgtc
      541 ggcggtgggc ggcgagccag gcgtcctcgg cggcccacag ggcgaggcgc tccgcgagga
      601 ggggtttggc gcggtcgagc acggtgagga agccgctgcg ctcgcggcca gggtcgtaac
      661 cgagggcgcc ggccgcgagg gcccatcact cctgtcaggt ctcggcgatg aactcgaagg
      721 tcaggccacc cggtgggatc ggtgcggcga ggcagcggcg ggggttcgac tggttcatcg
      781 cggtccagcc gtgcgcgggg ctgcagctcc acggggtgcc ggcgggggcg accaggcgga
      841 agcggcggcc gccggcggtg cggccgtgcc acacgctgcc gcgcagcagc agggcgcagc
      901 tctcggggaa gaacaggcgg gtggtcgcgg tcgtctcggg ctcgacgcgg tcctcgcaga
      961 ccaggcgccg gccgtcccag tgtaggcggc gatagtgcgt cgcggcggcg cggtagccgt
     1021 gcaggcgggc ttcgaggtga tctgtcccga aggacaggtc ggtggtgcgc gcgcggccgc
     1081 cgacgcggtg gctcgcccac atctcgtgtt gattactgtc gttgtcgccg atcgtgaccg
     1141 tgctgtgggc gcgggtgctg cgggcgtagt cgcgggcgaa gcccgggttg taggtgccga
     1201 cgccggcgtc gatgacgacg cgctcctcgt gcaacgagag ctcgaaggcg aggccgtcgg
     1261 cgtggatgtg gccgggctgg ttcggtaaac cgatgccgcc ggcgttccac agtaaagtcc
     1321 acgggccgaa gcggcggcgg aacaggccgg cgttcggggc gtcggcgggg ccggtgagaa
     1381 gggactcggg aggacgatcg aggcgggcga agaggctccg cggggtcacg gctgcgccct
     1441 gttgcgagtc gttccacagg gcgacgtcgc cgtccgggtg ggtgaacgcg ggcagggcgg
     1501 agatcatgcg gtcgagcgcg gcgtcgaggc gcgcggccgc ggcggggtcg accctcgcgg
     1561 ggttgagggc gacgacgccg gcgagctgct cggcgagcag gcagtggtag gtcggcgtgc
     1621 gctcgcggtg cacgccgtcg gggccgagct gggcctcgac gatgtcggcg aaggtggcga
     1681 tgtgccgcgt cagcggggcg gcggcggggc cctcgagcga gaggccggcg gagcacagcg
     1741 cggcgaggtc ggtccacacg tggttgccgc cgaggtggtg ctcgacgtgc acgcgcaggt
     1801 gctcgagctg ggccgcgagc gacccgtaga tccggcggcg ggcggtcgcg tcgaggtggg
     1861 actcgtgctt gccgagccag ccgagccagt gcagcacccg catcgcggtc gggtagggct
     1921 cccagccgac gccctcgagg tggttgtcga tccagtgctc gacctgggcg cgcgcgcggg
     1981 ccagcggcag gcgggataac cagccgtgat aatgcacggt gtagacgtag agcgggtcgg
     2041 cgtcgggctc ccagccgtcg gcgcaggggt cgaacagcgg gtgctcgagc aggcgcacgc
     2101 cgccgggctc gatcacgccc tcggcggacg gcggcggcag cgggacgacg acggcgatca
     2161 ggccctcgcg ggtggtcgag acggggcggc gcgcgggtcc gcgcaggcgg cggacgccct
     2221 gatagaacac ctggcgcggg gtcaggtgca cgagggtgcg cagccaggtc ccgggcttca
     2281 tgcgcgcgtg cctgatacgc cgacggtcag cagaccgacg gtgtcgacga ccatgaggcg
     2341 cagcaattgg tcagggcgca ggtgacggaa ctggcggtgg gcgacgagga tgacgacgat
     2401 gtcggcgctg gcgagcgcct gctcgagcgc gacgagcggg acgccgtcga tcgggctgcg
     2461 gtgcaggtgg ggttccacca gcatcaggtc gacctcgccc gaagcagcga gggcctgggc
     2521 gatgtgcagg gccgggctct cgcgcaggtc gtcgatgtcg ggcttgtagg cgagcccgag
     2581 gcacgcgatc ttcggcctgc gcaggcgcga ggcgagctgg tggacctggt cgagcacgcg
     2641 ctgggggcgg tcgtcgttga cgtggcgggc cgcgcggatc agcggggtgt cgagcggcgc
     2701 ggcggcggcg atgaaccacg ggtcgacggc gatgcagtgg ccgccgacgc cgggaccggg
     2761 gcgaaggatc tgtacgcgag ggtgacggtt ggcgaggcgg atcacctccc acacgtcgag
     2821 gccgaggcgg ccgcacacgt tggcgagctc gttggcgaag gcgatgttga cgtcgcgaaa
     2881 ggcgttctcc gacagcttgg tcagctcggc cacctcggcg ctgcagcggt gcacctggcc
     2941 gcgcgcgaag ccttcataaa attcggcgca gcgggcggtg caggcgtcgg tcaggccgcc
     3001 gacgatgcgc tcgttctcga cgacctcgcg gatgacgttg cccgggagca cgcgctcggg
     3061 ggcgtgggcg aggaagacgt cgcggccggg cacgaggccg ccgcgggtga tgatcggcgc
     3121 gacgagctcg cgcagggtcc cggggggcac ggtcgactcg agcacgacga ggcagccggg
     3181 gcgcagggcg ggcacgatcg cgtgggcggc ggcctcgacg tggcgcaggt cgggctggtg
     3241 atctggagtg atcggtgtgg gcacacagat caggaagttg cgggcggcga ccggctcgga
     3301 gacggcgcgc aggcggccgg ccgtgaccgc ggcgcgcacg agggcgtcga gctcgggctc
     3361 gacgatgtgg atccgcccgg cgttgatcgt gtcgagcacg tcgcggcgca ggtcgacccc
     3421 ggcgacgcgc cggccgttgg tggccaggac cgcggccgtc ggcaggccga cgtagccgag
     3481 gcccagcacg cacacgtcgt actcggaagt ttcatgcgca gcgggcgtgg acagggtcac
     3541 cttcgtggca tgctatcgac gacccgcgat gtcgtccacc cagccccgca tcctgatcgt
     3601 gttcggcacc cgcccggagg tgatcaagct ctggccggtc gaccgcgccc tgcgaagttt
     3661 ccctggtttc caggtgacgc tgtgcaacac cggccagcac cgcgagctgg tgccgccgct
     3721 cctggcgacc tttcgcatgg tgcccgaggt cgagctcggc gtgatgagcc cgggccagag
     3781 cctcagctcg ctgctggccc gcctgctgga cgggatcgat cccgtgctcg acgcgctgcg
     3841 cttcgactgg gtcatcgttc aaggtgacac cacgaccgcg ctcgcggcgg cgctggcggc
     3901 cgcgcaccgc cacgtgccgg tcgcccacgt cgaggccggc ctgcgcacca acgacctcct
     3961 gcggccgttc ccggaggagg gcaaccgcca gatcatcgct cgcgtcgcca gtttgcacct
     4021 ggcaccgacc gcgaccgcgc gcgcggccct gctgcgcgag ggggtgccgg ccgaacgcat
     4081 cgaggtgacc ggcaatacgg tgatcgacgc cgcgcgcgcg atggccgaga tgctgcccct
     4141 cgacggcgac ccaccgatcg gcagcccgct ggagctgctg cgcttcaccg gcgaccgcca
     4201 cgtcgtgctc gtcaccggcc accgccgcga gagttttgat ggcggactgg cgtcggtctg
     4261 cgaggggctc gcgatgctcg cgcgaaatca ccccgaggtc gacttcgtgt acccggtaca
     4321 cctcaacccg agcgtgcagt cggccgtgcg cggcacgctg gccgggctgg cgaacgtgca
     4381 cctgctcgaa ccgctcgact atcccgcggc gatctggttg ttacgcaggt gcctgttcgt
     4441 gatcaccgac tcgggcggac tgcaggagga ggcgccggag ttcggcaagc cggtgctcgt
     4501 cacccgcgcg agcaccgagc gcagcgaggg catcgcggcc ggctgcgcgg agctggtcgg
     4561 ctacgaccgt gaccgcctgg tcgccgccgc tgaacggttc ctgcgcgacc cggcctcggc
     4621 gcggctgacg atcgacaacc cgtacgggga tggccacgcg gcggagcgct gcgctgcggc
     4681 gctgcggcgc tgcctcggcc tgccggagga cgccccgcgg gtgctcgacg cggaggacat
     4741 cccgtgagag gtcgagcgtg agcgcgacgc cgtgggtgct cggcatcgcg ggcgcgctgg
     4801 ccctgctgct gacggcgctc ggcatgccgc ctgctgtccg cgggtggaac gcgctcggcc
     4861 gcaaagaccg cgagatcagc ctgcgcaagg tccatagcgg cgagatcccg cgggtcggcg
     4921 ggttcgtgct gatgctcggc ttcgcgatcg tggcgatccg tggtttggcg ggcacgaccg
     4981 agctgtcggc gacgcacgag tggttctcgc agaacccgct gaccggcgcg gtgctcgggg
     5041 cgctgatctg cggggcggtc ggattatatg atgacctcgt cgggctgcgc gcccgctaca
     5101 aattgttggg gcaggtgttc gcggcggcgc tggcggtgtt cggcttcggg ctgcactggg
     5161 aggcgctcgc ggccctgctc gggcccgcgg agatcctggc ggatccgctg acggtgctgt
     5221 ttttggttgc gggcgtcaac gcgatcaacc tcagcgacgg gctcgacggg ctcgcggccg
     5281 gcaacgtggc gatcgggcta tcggtggtgg tcgtcgcggc gatcgggcac ggcgcggcgg
     5341 cggtcccggt cgggtggatc gccgcgaccg cgctcggggc ggtgctcggg tttttaattc
     5401 acaaccgcca cccggcgcgc gtgttcatgg gcgacgcggg gagctacttc ctcggctttt
     5461 tgctgacggc gctgctgttg ctggtgcacc cggttcgcga gcgggtggca attatacagc
     5521 tgtcgatccc gctgatggcg ctggcgctgc cgctgttcga catgaccctg gcgatcgccc
     5581 gccgggccgc gcgcgggcag ccgatcttcg cgcccgactg cgatcatatt caccaccgat
     5641 tactcgcgcg cgggctgcca cacgcgcgcg tggtccagat cctgtggctg tcggcggcgc
     5701 tgttcgccgg gctcgcgcac ctcaatgtga tcggggtcgg cggctggtgg acgctgaccg
     5761 gcgcggtcgc ggcgatgctc gtagtcgcgg tgctgctcgg ctatcacaac atgctgcgct
     5821 cgctgccggc gttcaccggc gaccacctgc tcggcctgcg tgaccgccgg cgccaggtga
     5881 tggagatcct cgcggcgatc gacctgctcg ccggcgagac cagtgaccgc gaggagccgc
     5941 gctggcgccg gctgacaccc gagctcgcgc cgatcctcgc gcgcctcggg gtgcccggct
     6001 tcgaggttcg gcgcggcccc gaggtcgtcg cgcgcgccgg caccgacgag ggcgcgtggg
     6061 cgtggctcag cctgccgttg cccggctcgc ccggcgccga ggtccgcctc gcgctcgcgg
     6121 tgcgcctgcc ggaattgcag tcggagcagc tgatgctgct cgagcgcgtg gtcgcggtgc
     6181 tcgccggctc accgctcggc ggggcgcgct gacatgtctg aaaagacagg tgatgggcgg
     6241 tcggactcgg catgaccccg gacaaattca cggtggtcgg gaccttcggg tcggtcgcgg
     6301 aggccgagat ggcgcgcggt cagctggcgg cggcgggcct cgaggtgcgc ctcgacaacg
     6361 cgcagacgct cggcgtgctg ccgatgcaca gcctggcgat cggcggcgtc ggggtcgcgg
     6421 tggcgacccg cttcgccgcg caggcgcggg cgatcctcgg cttcgaggag gatggccgac
     6481 ccgaggagga caccgaaccc gactcggcga tcgccctgca agcgcccgac gcgtggatgc
     6541 gccgcgcggc ggtcatggcc gtcgtcggct cggcgttcat cctgccgacg ctgtactcgg
     6601 tgtacgtgct gggaaagtac ggacgcggca tcatgagccc gctcggcaag cgcaaccgtg
     6661 cgatcgcgat cgtcttcaat gcgttcgcgt tcgtgatcgt gacgctgttc gcgatcctga
     6721 gctgctgagt cattcgccga gctcgccggt cgactccgaa gcctcgggca ccgtggggcc
     6781 gggggcgagg ccggtcttca cctgctcggc gatctccggg agcgtgtagc cggtctcgac
     6841 ctgctgatga acccacgcgg cgcgctcgac cgcccagccc ggccagcgac tgtgtacccc
     6901 gccgcgacca cgactgatga tcgtgggctc tggcaaaagg gtcatcttac accatcggcg
     6961 cagcgtgtca cggtgaactt caccggccgc ggcggcgacc tcctgcgtgg tggcaaaggg
     7021 ctttggagct ttgaggcgag acggcatgac aaaaggcggt ggtcggcccg gagtctactt
     7081 tagttgcgcg ggagagtttg aaagatgagg acggggtctt gtgaagactg aaagggtgtg
     7141 caaggatgta tttggagtgc aacaaggatg tacaagcaca ggaacaatca tggaacgaag
     7201 cacggaacga agcggagacg aggcgtgcgt gggagcgccg ggctgcgggc gctcgcgggc
     7261 gggggggcgc agtacgccgt gctcgacgcc gcaggcgcag tggtgacgac ggcggcggcg
     7321 tgggatgacg acgcgccgag agcggcgcgc acgcgggacg tgctgctcgt gcatcgctgg
     7381 ttgcgggcgg cgcaggccgg cgacccggag ggcgggctgc agctcggggt acttcgcgga
     7441 tgcgcggggc cgtggcgggt gtgcgcgccg gtcgtgcggg tgccggtgct gccggagcgc
     7501 gagtgtcggc gaggggacgc gctgcgcttg ctggaggccg cgccgggacc ggcgctgcgc
     7561 gaccgggcga ggctgcgggt gacggggcta ctggcgtgcg ccggcctcga tcgcccggcg
     7621 gcgaggtccc tgctgcgagc ttacgagggc ttttttcccg gcgtggaggt cggggtcgcg
     7681 tggtcgctgc acgatcggct gtgtgcgcgc gggcgcagct gcctgcactg cctcgacctg
     7741 cggtcgttga cgtgggatct gctggccggc gcgagcctgt ggacgctccc ggagaccggt
     7801 ggggggcgct ggccggcggg tgtgcacctg ttctcggtcc acgcgggggc cgagacgtcg
     7861 cctggccgca ggggcaggcg gggtccggcg agctccgggc cgtcaattcc ccatgcttcg
     7921 ccgcattgag gcaacggggt tctcttctgg ttcttggctt ggcgggaccg ctcgcttaca
     7981 atgcgtcgcc ggtctgcgcg cacctcgctc tgatccactc ggaatccctc cggcggcacg
     8041 aactcgaaac gaaggcatgc gatgaagtgg gtcgagatca ggcacgatgg tgacggcaac
     8101 cgcggcgcgg cctatgtggc cgatgtgccc ggcggcgtgc tggtccggac ggtgacgcac
     8161 ctcattcttc atacgggcct ctacgtctcg gaggccctgg ccttcgtgcc gaacgtgacg
     8221 gtcgcggact tcgagcgcaa gccgaaggac gaggactagc ccgtcggcgt ccgggtcggc
     8281 ggagcccgga tggcgtcgca ggttcatgac aggcgtcggt tcctcgcgtt cgtgctgctc
     8341 gcggccgctt gcaagcccgc gccggccgag ccggcacgtc cgcaggcggt gctcgtgagc
     8401 cccgaggcgc gggccgacgc gcccacgtgg gtcgcaggcg gcgatgcatt tgcgatcgag
     8461 ctgtatcagc gggtgcgcgc gcgcgagggc aaccagttct tcgcgcccgg caacctgcgc
     8521 agcgccctgg cgatgaccta cgccggtgct gccggcacga ccgcgcagga gatggcgcgc
     8581 gcgctgcact tcgagtcctt gcctggcccg cgcctgcacg ccgcgcaggc ggccgcggcg
     8641 gcggacctcg ggacggcgga ctcgggggtc ggcggggtgg tgttgcgggc ggcgaaccgg
     8701 ctgtggggcg cgcagggcga gcccttcgtg ccggcgtatc tcgagctgac gcgcacgcag
     8761 tacggagccg agctggtcgg catcgacttc gcggcgaccg agcacgcgcg ccagacgatc
     8821 aacgcctggg tctcgcaaca gacgcaggcg aagatccccg agctgctggc ggcgccggac
     8881 atcgacgcgg cgacccggct ggtgctgacc agcgcgatct atttcaaagg tgcgtggctg
     8941 cgggccttcg acccggcgca gacgctggac cggccgttcc aggtcccggg cctggcagtt
     9001 ccgccggcgg tgccgacgat gcagcagaag gcggaatttt ttaagtacgc cgagcccgag
     9061 ggggtgcagg tgctcgagct gccgtacgtc ggtgaccgcc tggcgatgtt gatcgtgctg
     9121 ccgcgcgcgg ccgacggcct gcccgcgctc gaggcggagc tcagcccggc gcgcgtcgcc
     9181 gggtgggtcg cggcgctgcg gtctcgcaag gtccaggtcg ctttgccgcg cttcaccacg
     9241 cgcgcgcgct tcgagctctc gggcgtgctg gcggcgatgg ggatgccgac cgcgttcacc
     9301 ggcgcggcga atttttctga aatgtcgcgc gcgggcggcc tgtcgatctc gagcgtggtc
     9361 cacgaggcct ttatcgatgt cggcgaggcg ggcaccgagg ccgccgcggc gacggcggtg
     9421 gtcatgacgc gcagcatgcc gatcggcgcg ccgccggcgt tcacggccga ccgcccgttc
     9481 ctctacttca tccgtgacac tcgcagcggg cagctgctgt tcgttggtcg cgtcgtcgac
     9541 ccgcgcggct gatctctcag cgccggggct tggcgggcgt agcctcgggg ccgcgcacgg
     9601 tgatctcgta gcgctcgccg acctggcgca tcaccagctg cgtcggggcg cggtcggccg
     9661 ccagcgcgag cacggcggtg aacagcttgt cgatcgcgtt ctgctcgagc gcctgcaaca
     9721 ccgcggagac ggcctccggg ctggcggcgg aggtcgcgtg caggaggtcc ttcatgccct
     9781 ccttgcccga cgccgcgtcg ccggcctcgg tgagccacgc cttgagctcg ctgtccgcct
     9841 tcggtccgat gacgatcagc aaaccaccgg cgtgggcgct gccgttgaag gcgacgcgca
     9901 ggcggccgtc gtcgagcaga gggcgcagcg aggtcgggac ctggttgccg aggccgctcg
     9961 cggtcaacat ccagatgtcg gggttgtcgc cgaccttctt gagcgaggcg ttggggatgt
    10021 tcgacacgaa catgcgcgcg agctggaggt tctcggtgat gtcgcgcagc agggtcagca
    10081 gggcccggtg gccggcgtgc gggtcgtcgg cgccgaggcc gaggcgcagg tgcttggtcg
    10141 ggccgacagc caacagcacg cggccgttcc acatcttgag cacgcgcgtc gcctggtcgg
    10201 cgatcgggtc gagcatcatg cggcccgcga agccggcgcg gtcgaccatg acctgaagct
    10261 ggtgcgtgac gtcgcggacg gcctccttgt agccggtcca gcgcgtgctg acgccggcgg
    10321 cgatgctctt gctggtcagc gggccgccga tcgggccggg ggcgatgt
//
LOCUS       DASWNJ010000151         2107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_568179, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000151 DASWNJ010000000
VERSION     DASWNJ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568179"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VGB85_02850"
     CDS             <1..234
                     /locus_tag="VGB85_02850"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEY0132983.1"
                     /translation="KARDAAQVTWRVTNTAVHELGHVLGLDHCEEPRCVMLDAQGGIA
                     NTDESIGGPGPACRSKLDRASPLLRPAALRPAR"
     gene            313..1758
                     /locus_tag="VGB85_02855"
     CDS             313..1758
                     /locus_tag="VGB85_02855"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEY0132984.1"
                     /translation="MSGPSLALLTDLYQLTMAHGYWKLGRAEDEAVFHLFFRKPPFKG
                     SYAIAAGLAQAVEYLTRLRFDAGDLEYLATLTGNDGAALFDPGFLEYLGALRFTCEVD
                     AMPEGTAVFAHEPMLRVRGPILQCQLVETPLLNILNFASLIATKAARVCGAAGGEAVL
                     EFGLRRAQGIDGALSASRAAYVGGCSATSNVLAGKLYGIPVRGTHAHSWVMSFDDERT
                     AFAAYAEAMPNNCTFLVDTYDTLEGVRRAAEVGQQLRARGHKLAGVRLDSGDLAALSR
                     SARALLDAAGFPDAAIVASNDLDEHEISKLKRSGAQITVWGVGTRLVTAHDQPALGGV
                     YKLAAIRRDASQPWQYRIKLSEEPIKVSNPGIQQVRRYHDADGMFTGDVMYEETLGRG
                     AEARRLEDPSRSCEPAGEGEDLLVPVLRDGVRVGPAPTLAESRARTLAQLQRLPPGVR
                     ALQDAEAYPVGIDAALQALKETVMRAARGQR"
     gene            1803..>2107
                     /locus_tag="VGB85_02860"
     CDS             1803..>2107
                     /locus_tag="VGB85_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY0132985.1"
                     /translation="MVFGLDDEDLKVLLIQRDRAPFAGKWALPGGFVEPEETLEQAAL
                     RELAEETGVALKKRVFLEQLYTFGDPGRDPRDRVITVAYYGLVRRLDHAPRAASDARA
                     "
BASE COUNT          319 a          676 c          800 g          312 t
ORIGIN      
        1 aaggcgcgcg acgccgccca ggtgacatgg cgagtcacca acaccgccgt gcacgagctg
       61 gggcacgtgc tgggcctcga ccactgcgag gagccgcgct gcgtgatgct cgacgcgcag
      121 ggcgggatcg ccaacacgga cgagtcgatc ggcgggccgg gacccgcgtg ccggagcaag
      181 ctcgatcgcg cgtcgccact actacggcct gcggcgctgc ggcccgcgcg gtgacgtgca
      241 cgcatgcacg cttgacgggc ggggtcgcgg ctgccacaca tcttggtgtc gttcgtacac
      301 gaaggggtgt cgatgtcggg accgtcgctc gcgctgctca cggacctcta tcagctgacg
      361 atggcccacg gctactggaa gctcggacgc gcggaggacg aggcggtgtt ccacctgttc
      421 tttcgcaagc cgccgttcaa gggcagctat gcgatcgcgg ccgggctcgc gcaggcggtc
      481 gagtacctga cgcggctgcg cttcgacgcc ggggacctcg agtacctggc gacgctgacc
      541 ggcaacgacg gcgccgcgct gttcgacccg ggcttcctcg agtacctcgg cgcgctgcgc
      601 ttcacctgcg aggtcgacgc gatgcccgag ggcacggcgg tgttcgccca tgagccgatg
      661 ctgcgcgtgc gcgggccgat cttgcaatgc cagctcgtcg agacgccgct gctcaacatc
      721 ctcaacttcg cgagcttgat cgcgacgaag gcggcgcggg tgtgcggtgc ggccgggggc
      781 gaggcggtgc tcgagttcgg gctgcggcgc gcgcagggca tcgacggcgc gctgtcggcg
      841 tcgcgggcgg cgtacgtcgg cggctgctcg gcgacctcca acgtgctcgc cggcaagctg
      901 tacgggatcc ccgtgcgagg gacgcacgcg cacagctggg tgatgtcgtt cgatgacgag
      961 cgcacggcct tcgcggcgta cgcggaggcg atgccgaaca actgcacgtt cctggtcgac
     1021 acctacgaca cgctcgaggg cgtgcggcgg gccgcggagg tcgggcagca gctgcgcgcg
     1081 cgcgggcaca agctggcggg ggtgcgcctc gactcgggcg acctggcggc gctgagccga
     1141 tcggcgcgcg cgctgctcga cgcggccggc ttcccggacg ctgcgatcgt cgccagcaac
     1201 gacctcgacg agcacgaaat ttcaaaattg aagcgcagcg gggcgcagat cacagtgtgg
     1261 ggcgtcggca cccgcctggt caccgcgcac gaccagccgg ccctcggcgg ggtctacaag
     1321 ctcgcggcga tccgccgcga cgcgtcgcag ccgtggcagt accggatcaa gctgtccgag
     1381 gaaccaatta aggtgtcgaa ccccggcatc cagcaagtgc gcagatatca cgacgccgac
     1441 gggatgttca ccggggacgt gatgtacgag gagacgctcg ggcgcggggc cgaggcgcgt
     1501 cgcctggagg atccgtcgcg cagctgcgag ccggcgggcg agggcgagga cctgctggtg
     1561 ccggtgttac gcgatggggt tcgcgtcggt ccggcgccta cgctcgccga gtcgcgcgcg
     1621 cgcacgctcg cacaattgca gcgcctgccg cccggtgtac gcgcattgca ggacgcggag
     1681 gcctacccgg tcggcatcga cgccgcgttg caggcgctca aggagacggt gatgcgcgca
     1741 gcccgggggc agcggtgaga tttttttata aacatgcgcg accggcggtg acggtggatt
     1801 gcgtggtctt cgggctcgat gacgaggacc tcaaggtgct gctgatccag cgcgaccggg
     1861 cgccgttcgc cgggaagtgg gcgctgccgg gagggttcgt cgagccggag gagacgctcg
     1921 agcaggcggc gctgcgcgag ctggccgagg agacgggcgt cgctttaaaa aagcgagtgt
     1981 ttttggagca gctttatacc ttcggggatc cggggcgtga tccacgggac cgcgtgatca
     2041 cggtcgccta ttatgggctc gtgcgaagat tggaccacgc gccgagggcc gcgagcgacg
     2101 cgcgggc
//
LOCUS       DASWNJ010000152         2720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_532780, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000152 DASWNJ010000000
VERSION     DASWNJ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532780"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..1621
                     /locus_tag="VGB85_02865"
     CDS             89..1621
                     /locus_tag="VGB85_02865"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0132986.1"
                     /translation="MALPSPPAPGQQYEGALQFASPVGTVIAERYHVLKHLSDGGMAT
                     VYLAEHIAVGRRLAIKILHPDQACQPDVVRRFLQEARAASLVRNAHVVDIIDVGFTAD
                     GLAYMAMELLEGEDLGSLRDREGPMTWTRLGPMILQICDALAATHAQGIVHRDIKPEN
                     CFCTEFGGSHDFIKILDFGIAKDLGGQLDGDRPQTISGSIYGTAAYVAPELLAGKPAD
                     ARADIYALGILMYEMLLGERPFVGEALSEILIGHLQVPPTPLRSKIPDRVPIAVDSLI
                     LKTLAKDPADRFQSMSELSDAVAGTLDAGTLQRAVEAGAVPTGAYLAVLRRDASRAVP
                     RVPPSNRRRKLVLGSLAALTVVLAVFAVFWLVREPERVAAPGLPAPVAVSEVPPQAPR
                     APLQAPPPPRLDRVPPTPTRTPAATPAPARPTRLSLAGVRKEIQRKLAPRARECLAKH
                     TRLIKGQQFPVKVEILPSGEARATATLATSPAARCITDLFRRHRFPANIGGLKLDHAF
                     TP"
     gene            complement(1658..2128)
                     /locus_tag="VGB85_02870"
     CDS             complement(1658..2128)
                     /locus_tag="VGB85_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132987.1"
                     /translation="MRMGVLPRLNALLVAGAVATAPLACAGEQHLRAGSSVPTSAPRG
                     PQRTREQILNRDRALIGGLATSLALGLVGLGSLLANANLSGERDTAAPHEMPPMIAVA
                     GGLMSLGFLTSIPLGIAVERHRNRYADVFQPKRGPRPTASLSPKLLLRPAPATP"
     gene            2298..>2720
                     /locus_tag="VGB85_02875"
     CDS             2298..>2720
                     /locus_tag="VGB85_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132988.1"
                     /translation="MLACLVFSSLDRVDLVYTGPDGRVRWLQSDHRRPAQIESEAELS
                     LIFALLRLTDPRRDFPEGEPSPVLEYRCQHMPPAFLRAAIAAAGAELVVDGPVPFEDV
                     APDPALLLRLGLAALADRVARERELACDLAGLEQLEREQ"
BASE COUNT          369 a         1080 c          917 g          354 t
ORIGIN      
        1 tcccccgcat cgatgtgatc cccggcggcc tcggcctgcg cttttagccg acgcgcgccc
       61 agccacctgg ctaggatcgc cgggctcgat ggccctcccc tctcccccgg cgcccggcca
      121 gcaatacgaa ggcgcgctgc aattcgccag cccggtcggc accgtcatcg ccgagcgcta
      181 ccacgtgctc aagcacctga gcgacggcgg catggcgacg gtctacctcg ccgagcacat
      241 cgccgtcggt cgccgcctgg cgatcaagat ccttcacccc gaccaggcct gtcagcccga
      301 cgtcgtccgc cgcttcctgc aggaggcgcg cgccgcctcg ctggtgcgca acgcccacgt
      361 ggtcgacatc atcgacgtcg gcttcaccgc ggacggcctc gcgtacatgg cgatggagct
      421 gctcgagggc gaggacctcg gctcgctgcg cgaccgcgag ggcccgatga cgtggacgcg
      481 gctcgggccg atgatcctgc agatctgcga cgcgctggcg gccacgcacg cgcaggggat
      541 cgtgcaccgc gacatcaagc ccgagaattg cttctgcacc gagttcggcg gctcgcacga
      601 cttcatcaaa attctcgact tcgggatcgc caaggacctc ggcggccagc tcgacggcga
      661 tcgcccgcag acgatcagcg gatcgatcta cggcaccgcc gcctacgtcg cccccgagct
      721 gctcgccggc aagccggccg acgcccgcgc cgacatctac gcgctcggca tcctgatgta
      781 cgagatgctg ctcggcgagc ggcccttcgt cggcgaggcg ctgtcggaga tcctgatcgg
      841 ccacctccag gtcccgccga cgcccctgcg cagcaagatc cccgaccgcg tgccgatcgc
      901 cgtcgacagc ctgatcctca agacgctcgc caaggacccc gccgaccgct tccagagcat
      961 gagcgagctc agcgacgccg tcgccggcac gctcgacgcc gggaccctcc agcgcgccgt
     1021 cgaggccggc gccgtgccga ccggggccta cctcgcggtg ctgcgtcgcg acgcctcgcg
     1081 ggccgtgccc cgggtcccac cgagcaaccg ccgccgcaag ctcgtgctcg gctcgctcgc
     1141 cgcgctcacc gtggtgctcg cggtgttcgc ggtgttctgg ctcgttcgtg aaccggagcg
     1201 agtcgccgcg ccgggcctgc ccgcccccgt cgcggtgagc gaggtgcccc cacaggcccc
     1261 ccgggcgccg ctgcaggccc cgccgccgcc gcgactcgac cgcgtgccgc cgacaccgac
     1321 gcgaacaccg gccgccacac cggcgcccgc gaggccgacg cggctgtcgc tcgccggcgt
     1381 ccgcaaggag atccagcgca agctggctcc ccgcgcgcgc gagtgcctgg ccaagcacac
     1441 gcgcctgatc aaaggtcagc agttcccggt caaggtcgag atcttgccca gcggcgaggc
     1501 ccgggccacc gcgacgctgg cgaccagccc ggccgcacgc tgcatcaccg acctgttccg
     1561 ccgccaccgc ttccccgcca acatcggcgg cctcaagctc gaccacgcgt tcacgccttg
     1621 aagcggggcg gggcggggcg gagcggcgcg gcgcttttca cggcgtcgcc ggggccgggc
     1681 gcagcagcaa cttcgggctc agcgaggcgg tcggacgcgg cccacgcttc ggctggaaca
     1741 cgtcggcata acggttgcga tggcgctcga cggcgatgcc gagcgggatc gaggtcaaaa
     1801 agccgaggct catcaggccg cccgcgaccg cgatcatcgg cggcatctcg tgcggcgccg
     1861 cggtgtcgcg ctcgccggac aggttggcgt tggcgagcaa cgaaccgagg ccgacgagcc
     1921 cgagcgcgag gctcgtcgcg agcccgccga tcagcgccct gtcgcggttg aggatctgct
     1981 cgcgcgtgcg ctgcggacct cgcggcgccg acgtcggcac gctcgacccg gcccgcagat
     2041 gctgctcgcc ggcacaggcc agcggcgccg tggcgaccgc accggcgacg agcagcgcat
     2101 tgagacgagg cagcacgccc atccgcatct cgccatccta ccacagtccc cacacgcccc
     2161 ggaaccggac cccggtcgga cctcgaacgg cccggcggac gcgctgtcag gccgctttcg
     2221 cccgcctgcg cgcgctcgct gcggggcagg cgcccggccg cgacggcgag cgccgccgcc
     2281 agcgccacgt cgggggcgtg ctagcgtgcc tcgtgttctc gtcgctcgac cgcgtcgacc
     2341 tcgtctacac cggccctgac gggcgcgtgc gctggttgca gtccgaccac cgccgcccgg
     2401 cgcagatcga gtccgaggcc gagctctcgc tgatcttcgc gctgctgcgg ctgacggacc
     2461 cgcggcgcga cttcccggag ggcgagccgt cacctgtcct cgagtacagg tgccagcaca
     2521 tgccgcccgc gttcctgcgc gccgcgatcg cggccgccgg cgccgagctg gtcgtcgacg
     2581 gtcccgtgcc cttcgaggac gtcgcgccgg atcccgcgct gctgctgcgc ctcggcctcg
     2641 ccgcgctcgc ggacagggtc gcacgcgaac gcgagctcgc ctgcgacctc gccggcctcg
     2701 agcagctgga gcgcgagcag
//
LOCUS       DASWNJ010000153         3405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_250313, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000153 DASWNJ010000000
VERSION     DASWNJ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250313"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(992..1684)
                     /locus_tag="VGB85_02880"
     CDS             complement(992..1684)
                     /locus_tag="VGB85_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132989.1"
                     /translation="MSLPTKLAAPQLVTNSRMLYTSWIPADPQACAALLPKALRPAAS
                     GAVYMNQYVVDSAEQTTGFGAYSLTYLGLDLDSFAPDGVTPARFFTHYFNSSEVVRAY
                     ASERGVPASPGTTELELHGRTLTATTHVDGRPLIRTRAHVGPKIAVTVAGHLRYVTRV
                     GDRLVGGNYPYIGELAEDFKVESIEFLDPHHPQFALRPATPQQVVPGACFYAPRDSFV
                     YPGGEVDMGAAD"
     gene            1797..2408
                     /locus_tag="VGB85_02885"
     CDS             1797..2408
                     /locus_tag="VGB85_02885"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0132990.1"
                     /translation="MPEIRALERRHVPEAGRLLGRAFVDNPAQLAALARLTPARRARV
                     VEAIHASFCAAAVSHWTAEALYEGEQMLGVMLALDPGVYPPSLRAKLTALRGALGAGL
                     HGLRNYLRIDEHMQGLHPTYPHYYLFIVGVDPPQQGRGHGRTLLEALNRRADGRGVPC
                     YLETDRASSVHLYESVGYRVLTDVRLAAVGGLRMWTMRRDPAR"
     gene            2842..>3405
                     /locus_tag="VGB85_02890"
     CDS             2842..>3405
                     /locus_tag="VGB85_02890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0132991.1"
                     /translation="MTQPSSEGIVLIVDDTPANLGVLHGALSREQLDVLMASDGVTGL
                     EVARSEAPDLILLDVVMPELDGIEVCRRLKADPRTHDIPVMFMTALGDTRDRLLGFEV
                     GAVDYITKPFQEGELIARIRTQLSLRRMTRALQLQNLHLEEQIRERTAAQAAREKLTE
                     ELRAAYGRLEEELAMRAQAEATRVRLQE"
BASE COUNT          487 a         1099 c         1312 g          507 t
ORIGIN      
        1 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg gttttgggga
       61 gcgcgggtgc atcgggaggg cgtgagcggg ttctgggagc gcgggtgcat cgggagggcg
      121 tgtggcgggt tcggggagcg cgggtgcatc cggaggacgt gtggcgggtt cggggagcgc
      181 ggggcgaggc tatctgggcc ggggcgcggg ggccgacagg cagcggggcc ctgcgacgcg
      241 ggtcctccca gggcacgtgc tcgccgcccc acctgtccgg aaggacatgc ttctttcact
      301 gcggctgtct ctccgctcgt gcgcggcggt ggggcgtctg cgcgcgagcc ctgggtcctc
      361 ccgctgcttc ccccgccagt cggcccctgc gccccgggcg agcatcgtct gcgccccgct
      421 ggtcgtgagg ccgataccgt cgaggatact tacagcgctg ctacggcagc agcagcaact
      481 gctgctggaa aatcgtggac gtgccgcgac ccgcctcgtg ctcgtcactt tatctgcgca
      541 tcatgatcgt gtggccaata cttcgaggac aaccattgcg catcgccgtg gcacgatcgc
      601 tgctcgtggc aggtgatacg ccgatttcgg cgcgcgagat ttcggactcg cgacacgatc
      661 cgtgcttgtg acgagcgggg cgcagacagt gcccgcccgg ggcgcagggg ccggctggcg
      721 ggggcagcag cggggggccc cagggctcgc gcgcagacgc cccaccgcca gtcgagagcg
      781 gcgagacagt ggcggtgaaa gagacatgtc cttccggaca ggtggggcgg cgagcacgtg
      841 ctctgggagg acccgcgtcg cagggccccg cagccggccg gcccccgcgc cccggcccag
      901 acagcctcgc cccgcgctct ccagccgcgc cctgtgctcc cgatagccgc gctcgcgacg
      961 agcccgtgcg ccagccgcga gcgacgaagc gtcagtccgc cgcccccata tcgacctcgc
     1021 cgccggggta cacgaaggag tcgcgcgggg cgtagaagca ggcgccgggc acgacttgct
     1081 ggggggtggc cgggcgcagg gcgaactggg ggtggtgggg gtcgaggaat tcgatcgact
     1141 cgaccttgaa gtcctcggcg agctcgccga tataggggta gttcccgccg acgaggcggt
     1201 caccgacgcg ggtgacatag cggaggtggc cggcgacggt caccgcgatc ttcgggccga
     1261 cgtgcgcgcg cgtgcggatc aacgggcggc cgtcgacgtg ggtggtcgcg gtcagggtgc
     1321 gcccgtgcag ctcgagctcg gtggtgccgg ggctggccgg cacgccgcgc tcgctggcgt
     1381 aggcgcggac gacctccgac gaattgaagt agtgcgtgaa gaagcgggcc ggggtcacgc
     1441 cgtcgggcgc gaaggagtcg aggtcgaggc cgaggtaggt cagcgaatag gcgccgaagc
     1501 cagtggtctg ctcggcggag tcgaccacat attgattcat gtacaccgcg ccgctggcgg
     1561 ccggacgcag cgccttcggc agcagagccg cgcaggcctg cgggtccgcc gggatccagc
     1621 tggtgtagag catgcggctg ttcgtgacga gttgaggggc ggcgagcttc gtgggtagag
     1681 acatgatggg cacgcagcat aatgggtcgc gctgggcggt ggaagtcacg cgatttgcgc
     1741 gacgccggtg cgcgtgcgcg ccgacggatc gcggcagtat agggtatgct ccgcgcgtgc
     1801 cagagatccg cgcgctcgag cgtcgtcacg tccccgaggc cggccgcctg ctcggccgcg
     1861 ccttcgtcga taaccccgcg cagctggccg cgctcgcccg actcacgccg gccaggcgcg
     1921 cgcgcgtcgt cgaggcgatc cacgccagct tctgcgccgc ggcggtcagc cactggaccg
     1981 cggaggcgct gtacgagggc gaacaaatgc tcggtgtcat gctcgcgctc gatccgggcg
     2041 tctatccgcc gagcctgcgc gccaagctga ccgcgctgcg cggggcgctc ggggccggac
     2101 tgcatgggct gcgcaattac ctgcgcatcg acgagcatat gcagggcctg catcccacgt
     2161 acccgcacta ttatctgttc atcgtcggcg tcgacccgcc gcagcagggg cgcgggcacg
     2221 gccggacgct gctcgaggcg ctcaaccggc gcgccgacgg ccgcggtgtg ccgtgttacc
     2281 tcgagaccga ccgcgccagc agcgtgcacc tgtacgagag cgtcggctac cgcgtgctga
     2341 cggacgtccg cctcgcggcg gtcggtggcc tgaggatgtg gaccatgcgc cgcgaccctg
     2401 ctcgctgaca cacgctcgcc ttcgcccgga ggccgcgtat gtgcgagggc tccatcgcgg
     2461 cgacatgtat gtcgacatgc tccgtcgagc acagccgggt ttacccccag gcttcatgcg
     2521 ggcggagaat cgaaggactt cgatatcacg cgatctgccg cgtcatgttc cgcactgtgg
     2581 cggtggcgac aatgttctgg cggacggtct gcggcccagc tggccggcga tcttgggtga
     2641 cggcgacatc atcgggggcc ggcagccgca gccgcgcagg gggcattttg cggttgccat
     2701 gcctggagcg agcccctatt gtccggggcg tatgtgccgg cagcccagtg gttaatgggg
     2761 gcggaaccgg accctatctc agcgcgtcga tcttcgagta taggcgatcc ccttcggctc
     2821 agttttgcaa gcgagcgagc aatgacgcaa ccttcgagcg agggcatcgt gctgatcgtc
     2881 gacgacaccc cggcgaacct cggcgtgttg cacggggcgc tgtcgcgtga gcagctcgac
     2941 gtgctgatgg cgagcgacgg cgtcacgggc ctcgaggtgg cccgcagcga ggcgccggac
     3001 ctgatcctgc tcgacgtcgt catgccggag ctcgacggta tcgaggtctg ccgccggctc
     3061 aaggcggacc cgcggaccca cgacattccg gtgatgttca tgaccgcgct gggcgacacg
     3121 cgcgaccgcc tgctcggctt cgaggtcggc gccgtcgatt acatcaccaa gccgtttcag
     3181 gagggcgagc tgatcgcgag gatccggacc cagctgtcgc tgcgtcggat gacacgcgcc
     3241 ctgcaactgc agaacctcca cctcgaggag cagatccgcg agcgcaccgc ggcccaggcc
     3301 gcccgcgaga agctgacgga ggagctgcgc gccgcctacg ggcgactgga ggaggagctg
     3361 gcgatgcgcg cgcaggccga ggcgacgcgc gtgcgcctgc aggag
//
LOCUS       DASWNJ010000154         6139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_462397, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000154 DASWNJ010000000
VERSION     DASWNJ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6139
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462397"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VGB85_02895"
     CDS             <1..448
                     /locus_tag="VGB85_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132992.1"
                     /translation="TPTAGGPDDARVLPPTPDTTTRVKPPPRAMKVQRMTPRRQLFQS
                     RLPYTALLDASFGVTLAGVAAAGLGAGLYIYGLECKEGPPRCSEVAPTGVRDAGLVLI
                     SAGAATMIIGFGLRFADHRAARKAIRNMPRPAASPTAMGLTWAARF"
     gene            complement(554..2581)
                     /locus_tag="VGB85_02900"
     CDS             complement(554..2581)
                     /locus_tag="VGB85_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY0132993.1"
                     /translation="MTTEPITHCDPKPPGLPASHLRLAGAVTLGCALLGACDETPGRA
                     APGPSEAPSATTPREGQQADDDEPITVPADQCPDDRRIVRAPPAACVSVPGDHGHWNA
                     VQLFADGTPSLAGAGTPPPGLAGYCQLTWAPDTSHAAPDLQVLGPLGAHESDCEVVWQ
                     QSDVLSDALSPALSATYLDHLDAPEAGDLLGARHPVVVAVIDSLSKATADGRSSHGES
                     VGALIDGVQCPDAACDSRVRYRLALPRYLNQQDETQVDWVKGGNFGSQGELAKAIFGA
                     VREWENHGSAGHLILNLSVGWEPSFGGPGAPADMPAPVRAVFDAMQYASCKGALIVAA
                     AGNAAGACEEGPMAPAMWENHLAPSNAVCETIGAPAAPGRPAITPNDRLVYAVAGLRH
                     DGRLLASTREKGRPRLAAPAFVAVNDAAGAPNIPMTGSSLAAASVSAVASLVWSYQHT
                     LSPGAVIQQIYAAGVADVATTLADFGVKQVTHRVSACKALAACTTTPTSECPAVVCDD
                     VPPDIIGLGAASTAAFAGLATPPVNVSLGNFSSKECEDACGTKITVRTAPGVLGDPCE
                     RDAVIGLNRSTSPQPRGTICPACFITNGAPDTAYLVIEEKYDLHTIAGVNLTVITEGK
                     EHPIELGVLALSSSKPLTVRLPADLIDATSTATLHVTFADGEIQDNEIPVY"
     gene            complement(2710..3348)
                     /locus_tag="VGB85_02905"
     CDS             complement(2710..3348)
                     /locus_tag="VGB85_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0132994.1"
                     /translation="MNSLTRILPALLVSVSVAAVALPGEAQAAPCGSSAATIAADTWQ
                     EFHNEAIAFGCAVGVALGTTSYTTCYDNLTYFAELGESLVAWWNDMANNSWATIGPRQ
                     LQWSGIMNGTLQSGAASRLFCSATPSNKETVDITIEKLDGKAQTEVEICKMHKNGTAT
                     VTHTFTFANGDANIGTVKTRTVTGAKDDIICVHLNNQSVGYSFQYSLDADKF"
     gene            complement(3531..>6139)
                     /locus_tag="VGB85_02910"
     CDS             complement(3531..>6139)
                     /locus_tag="VGB85_02910"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEY0132995.1"
                     /translation="PVCTLTSEPTTLRLWLAVAPDHLLVVEVDGQRMPAEARALAGGM
                     QLQVTVPVGSSQLVVRDTSAAAAWSLPLQPSLRVPALRLAWALLKWGKPAAADVVAAA
                     IEPLLTPLAAIEARTVRRKAALRRAMTTEDPAARDVALAESLRHADELVRRAGELGLV
                     SAQSESALASAWIALDQQRDLLSARTWLERQAPATLDDPQARAAAPYYAGLLAHAAGD
                     LREALHLLSEAELRTHRLGLVREEQAADTDYTVLLAELGRNDEVLARARAPAAWRDPC
                     DRAVRLNNAGWSLLLLAEAGHESGDPAQPLEQALALSEPGGACPNLGVVELVRINLAF
                     AALARGDPEEARPLLDVLHSEAPLGDVAAWLPELDGRVALQEGRPIADAELMAREPVD
                     GDEAELGPRWSSALRRGQALARRGLADAAIDAYGEAEVLLDRMVQTVGVDQGRELLLA
                     GKQRSARALVDLLVDRGRPEEALCAARLARLRGLRALDRAARLTALGPDVRARWDQAI
                     ADYQRNRQRAAELQRDAWRHVGAGAEHNRRLQQDAAESAARALDRAYAALGDHATPRC
                     LDLPPPAPGELVLFIFAGETDWIAFAADTTGVAAARLPAPQSRDLAATSRALLDPFAD
                     RLAAAARLRVLTSGIPWDIPVHALPWQGDIVLSAWPVSYALDLPGPRAPTAAGHEALV
                     IADPTGNLAHARDEARSVAATLGAQGWHVARLEQREATGAAVLAALAGADLLHYAGHG
                     SRDGLSGWGSVLALADDDTLAIHDVLALSRVPQTVVLSGCETAASDAAALAGGMNLAR
                     AFLIAGATAVIAADEVIDDALAAELSTRLHDGLAPGAVVDAPARLRHALLELRRARPG
                     SQEWAKFRALVP"
BASE COUNT          776 a         2038 c         2472 g          853 t
ORIGIN      
        1 gacgccgacc gcgggcgggc cggacgacgc gcgcgtgctg ccgccgacgc cggacacgac
       61 gacgcgggtc aagccgccgc cgcgggcgat gaaggtccag cggatgacgc cgcgccggca
      121 gctgttccag tcgcgcctgc cgtacacggc gctgctcgac gcgagcttcg gcgtgacgct
      181 ggcgggcgtc gccgcggcgg ggctcggcgc ggggttgtat atctacgggc tcgagtgcaa
      241 agagggcccg ccgcgctgca gcgaggtcgc gccgacgggc gtgcgcgacg cgggcctggt
      301 gctgatcagc gccggcgcgg cgacgatgat catcggcttc ggcctgcgct tcgcggacca
      361 ccgcgcggcc cgcaaggcga tccgcaacat gccgcgcccg gccgccagcc cgacggcgat
      421 ggggctgacg tgggcggcga ggttttaaaa tgtgaggaca tgtccggagg gacagggccg
      481 cgggccatgg ggtcaggcgg gcccggggtc ggaacaactc gatcgagccc gctgtcacgc
      541 gggtccgcga tcttcagtag acagggatct cgttgtcctg gatctcgccg tcggcgaagg
      601 tgacgtggag cgtcgcggtg ctggtcgcgt cgatgaggtc cgccgggagc ctgaccgtga
      661 gcggcttgct ggacgacagg gccaaaacgc cgagctcgat cgggtgttcc ttcccctcgg
      721 taatcacggt gagattgacg ccggcgatcg tgtgcaggtc atatttctcc tcgatgacga
      781 ggtaggcggt gtccggcgcg ccgttggtga tgaagcaggc cgggcagatc gtgccccggg
      841 gctgggggct ggtcgagcgg ttgagaccga tcaccgcgtc gcgctcgcac gggtcgccga
      901 gcacgccggg ggccgtacgg acggtgatct tcgtaccgca ggcgtcctcg cactctttcg
      961 agctgaagtt gccgaggctg acgttgactg gcggcgtggc gaggccggcg aacgctgcgg
     1021 tcgaggccgc gccgaggccg atgatgtcgg gcgggacatc gtcgcagacg acagcgggac
     1081 attcgctggt gggcgtcgtc gtgcaggccg cgagggcctt gcaggcggac acccggtggg
     1141 tcacctgctt cacgccgaag tcggccaggg tcgtggcgac atcggcgacg ccggccgcgt
     1201 agatctgctg gatgacggcg ccgggcgaca gcgtgtgctg gtaggaccat accagggacg
     1261 ccaccgcgga cacggaggcg gcggccagcg agctcccggt catcgggatg ttcggggcgc
     1321 cggccgcgtc gttgacggcg acgaaggcgg gcgcggcgag ccgcgggcgg cctttctcgc
     1381 gcgtgctggc gagcaggcga ccgtcgtgac gcagccccgc gacggcatag accaggcggt
     1441 cgttgggggt gatcgccggt cgtccggggg ccgcgggggc cccgatggtc tcgcagaccg
     1501 cgttgctcgg cgcgaggtgg ttctcccaca tcgcgggggc catgggccct tcctcgcagg
     1561 cgccggcggc gttgcccgcg gcggcgacga tcagcgcgcc cttgcaggag gcgtactgca
     1621 tggcgtcgaa gacggcgcgc acgggcgccg gcatgtcggc gggcgccccg gggccgccga
     1681 agctcggctc ccagccgacg ctgaggttca agatcaggtg gccggcgctg ccgtggttct
     1741 cccactcgcg cacggccccg aagatcgcct tggccagctc accctgactg ccgaagttcc
     1801 cgcccttcac ccagtcgact tgcgtctcgt cctgctggtt gaggtagcgc ggcagcgcga
     1861 ggcgatagcg gacgcgcgag tcacaggccg cgtccgggca ctgcacgccg tcgatcaacg
     1921 cgcccacgct ctcgccgtgc gacgagcggc cgtcggcggt cgccttggac agcgagtcga
     1981 tgaccgcgac caccaccgga tggcgggcac ccaggaggtc gccggcctcg ggggcgtcga
     2041 ggtggtcgag gtaggtcgcg ctcagggccg gcgacagcgc gtcgctgagc acgtcgctct
     2101 gctgccacac gacctcgcag tcgctctcgt gggcgccgag ggggccgagc acctgcagat
     2161 ccggcgccgc gtggctggtg tcgggagccc aggtgagctg gcagtagccg gccaggcccg
     2221 gcggcggcgt cccggcgccg gcgagcgagg gggtgccgtc ggcgaacagt tgcaccgcgt
     2281 tccagtggcc gtggtcgccg ggcacgctca cgcaggcggc cgggggcgcg cgaacgatgc
     2341 ggcggtcgtc ggggcactgg tcggcgggga cggtgatcgg ctcgtcgtcg tcggcttgct
     2401 gaccctcgcg tggggtcgtc gcggagggag cctcgctcgg gccgggggcg gcgcgccccg
     2461 gtgtctcgtc gcacgcgccg aggagggcgc agccgagagt caccgcgccc gcgagccgga
     2521 ggtggctggc gggcaggccc ggaggtttcg gatcgcagtg agtgatgggc tcggtcgtca
     2581 tgccggccca ttgtcggcga gctttccggc tctgacatgg ctcgcgaaac agcctccggg
     2641 gcgcggcgcc ccgggtgcgt tctcgcccgg cgacgggctg tcgtgcgacc ggggggtcgc
     2701 gggtggatct cagaacttgt cggcgtcgag cgagtactgg aacgagtagc cgacggattg
     2761 attgttgagg tggacgcaga tgatgtcgtc cttggcgccg gtgacggtgc gcgtcttgac
     2821 ggtgccgatg ttggcgtcgc cgttggcgaa ggtgaaggtg tgggtcacgg tggcggtgcc
     2881 gttcttgtgc atcttgcaga tctcgacctc ggtctgggcc ttgccgtcga gcttctcgat
     2941 ggtgatgtcg acggtctcct tgttggaggg cgtcgcggag cagaacaggc ggctggcggc
     3001 gccgctttgc agggtgccgt tcatgatgcc gctccactgc agctgacgcg ggccgatcgt
     3061 cgcccagctg ttgttggcca tgtcgttcca ccaggcgacc aggctctcgc cgagctcggc
     3121 gaagtaggtg aggttgtcgt aacaggtggt gtagctggtg gtgccgaggg cgaccccgac
     3181 ggcgcagccg aacgcgatcg cctcgttgtg gaactcttgc caggtgtcgg cggcgatcgt
     3241 cgcggcggag ctgccacagg gagcggcctg ggcctcgccg ggcagggcga cggcggcgac
     3301 ggagacggac acgaggaggg cggggaggat gcgagtgagg ctgttcatgg cgatggttct
     3361 ctgaaaaaaa agagcgatgt ggatgggatg cgctcgcgga tcgcgaggcg ctcgaacgac
     3421 gggagatcgc gggcggggcc tttgctgcgt gcgcgggccg gcggcgcctc gacaccgact
     3481 tcattgccgg cgagcgccgc ttcgatgaca aaaaaagatc ggtcgctcgt tcacggcacc
     3541 agcgcgcgaa acttggccca ctcctgcgag cccggtcgag cgcggcgcag ctcgagcagc
     3601 gcgtggcgca ggcgggcggg cgcgtcgacg acggcgccgg gagcgaggcc gtcgtggagg
     3661 cgcgtgctga gctccgccgc gagggcgtcg tcgatgacct cgtcggcggc gatcaccgcg
     3721 gtcgcgccgg cgatgaggaa ggcgcgggcg aggttcatgc cgccggcgag cgccgccgcg
     3781 tcgctggcgg cggtctcaca gccggacaac acgacggtct gcgggacccg cgacaaggcg
     3841 agcacgtcgt ggatcgcgag ggtgtcgtcg tcggcgagcg cgagcacgct gccccatccg
     3901 ctcaggccgt cgcggctgcc gtgcccggca tagtgaagga ggtcggcgcc ggcgagcgcc
     3961 gccaagaccg cggcgccggt ggcctcgcgc tgctcgagcc gggccacatg ccagccctgc
     4021 gcgccgagcg tggcggcgac ggagcgcgct tcgtcgcgcg cgtgcgcgag gttgccggtg
     4081 gggtcggcga tgacgagggc ctcgtgcccg gcggccgtcg gtgcgcgtgg tcccgggagg
     4141 tcgagcgcgt agctgacggg ccacgccgac aacacgatgt cgccctgcca cggtagcgcg
     4201 tgcacgggga tgtcccacgg gatgcccgag gtcagcacgc gcagtcgcgc ggcggcggcc
     4261 aggcggtcgg cgaaggggtc cagcagggcc cgcgaggtcg cggcgaggtc gcgcgactgg
     4321 ggggcgggca ggcgggccgc ggcgacgccc gtggtgtcgg ccgcgaaggc gatccagtcg
     4381 gtctcgccgg cgaagatgaa gagcaccagc tcacccgggg ccgggggcgg gagatcgagg
     4441 cagcggggtg tggcgtggtc gccgagcgct gcgtaggccc ggtcgagggc gcgggcggcc
     4501 gactcggcgg cgtcctgttg cagccggcgg ttgtgctcgg cgccggcgcc gacgtggcgc
     4561 caggcgtcgc gctgcagctc ggccgcgcgc tggcggttcc gttgatagtc ggcgatcgcc
     4621 tggtcccagc gcgcgcggac gtcgggtccg agcgccgtga gcctggcggc ccggtcgagg
     4681 gctcgcaggc cgcgcagacg ggcgagccgc gcggcgcaca gggcctcctc cggacgtcca
     4741 cggtccacca ggaggtcgac cagcgcccgg gcgctgcgct gcttgccggc gagcaggagc
     4801 tcgcgcccct ggtcgacgcc gacggtctgg accatgcggt cgagcaggac ctcggcctcg
     4861 ccgtaggcgt cgatcgccgc gtcggcgagg ccgcgtcgcg cgagcgcctg accgcggcgc
     4921 agcgcgctgc tccagcgcgg tccgagctcg gcctcgtcgc cgtcgacggg ctcacgcgcc
     4981 atcagctcgg cgtcggcgat cgggcggccc tcttgcaggg cgacgcggcc gtcgagctcg
     5041 ggcaaccatg cggcgacgtc gcccagcggc gcctcgctgt gcaacacgtc gagcagcggt
     5101 cgggcctcct cggggtcgcc gcgggccagg gccgcgaacg cgaggttgat ccgcacgagc
     5161 tcgaccaccc ccaggttcgg gcaggcgccg ccgggctcgc tgagcgcgag cgcctgctcg
     5221 agcggctgcg ccgggtcgcc ggactcgtgg ccggcctcgg ccagcaagag cagcgaccac
     5281 ccggcgttgt tgagccggac ggcgcggtcg caggggtcac gccaggccgc gggtgctcgt
     5341 gcgcgcgcga gcacctcgtc gttgcgtccg agctcggcga gcagcacggt ataatcggtg
     5401 tccgcggcct gctcctcgcg gacgaggccg agccggtgcg tgcggagctc ggcctcgctc
     5461 agcaggtgca gcgcctcgcg caggtcaccg gcggcgtggg cgagcagccc cgcgtagtag
     5521 ggcgcggcgg cccgcgcctg cgggtcgtcc agggtcgcgg gtgcctgtct ttcgagccat
     5581 gtcctcgcgg acagcaggtc gcgctgctga tcgagcgcga tccaggccga ggccagcgcc
     5641 gactcgctct gcgcgctgac caggccgagc tcgcccgcgc ggcggacgag ctcgtcggcg
     5701 tggcgcaggc tctcggcgag ggccacgtcg cgcgcggcgg ggtcttcggt cgtcatggcc
     5761 cggcgcaggg cggccttgcg tcgcaccgtc cgcgcctcga tcgcggccag cggcgtcagc
     5821 agcggctcga tcgcggcggc caccacgtcg gccgcggccg gcttgcccca cttgagcagc
     5881 gcccacgcga ggcgcagcgc cgggacgcgc aggctcggct gcaggggcaa ggaccacgcg
     5941 gcagcggcgc tggtgtcgcg gacgacgagc tggctgctac cgaccgggac cgtgacttgc
     6001 agctgcatac cacccgcgag cgcgcgcgcc tcggcgggca tgcgctgacc gtcgacctcg
     6061 accacgagca ggtgatcggg cgcgacggcc agccacaggc gcagcgtggt cggctccgag
     6121 gtgagcgtgc agacgggtc
//
LOCUS       DASWNJ010000155         2870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_532869, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000155 DASWNJ010000000
VERSION     DASWNJ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532869"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VGB85_02915"
     CDS             complement(<1..908)
                     /locus_tag="VGB85_02915"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0132996.1"
                     /translation="MGVASEIVLVTGGAGFIGSHVVDRLLAQGREVVVVDNFAAGKRE
                     NLAAHANSRHLRVIDADLCDDFAAALGGVRPAAVVHLGAQVSVPRSVEDPIADLDLNL
                     RASVRLLQWAAGAGARRVVLASSAAVYGEPARVPVSEDMCDRPASPYGASKRAAELFF
                     ACLGPALGVETAALRPFNVYGPRQDPRSAYSGVVSQFLARATAGEPLVVLGDGAQTRD
                     FVYVEDVARAFVAAIDAPGIDGLVANLGSGHSHTIAALAAEIVAVTGSTSPVVFGAAR
                     AGDVAHSLADCGRAADRLGWRAEVPLA"
     gene            complement(916..1770)
                     /locus_tag="VGB85_02920"
     CDS             complement(916..1770)
                     /locus_tag="VGB85_02920"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEY0132997.1"
                     /translation="MAQVEVFARTEVGCVRKRNEDHFVVANLATGELGLQPSARVQPL
                     TLAGTLVAVCDGMGGAAAGDVASNIAADTLAKLLQQYSPFADMAAAEIAMQAAVTAVN
                     NAIRGAAADNPMRHGMGTTMTAALAIGAQLLIGHVGDSRAYLRRGRALTQLTTDHSIV
                     GQLIAAGRLQPDQARNYEHRNVLLQALGVQPRVGPEIVQAQLRAGDVLLLCSDGLTGP
                     IADDMILELMLRYQDPVRCCRALTEAACAAGGPDNVTVAIARFTGDGLELPQGREEVA
                     MLRVQHSI"
     gene            complement(1771..>2870)
                     /locus_tag="VGB85_02925"
     CDS             complement(1771..>2870)
                     /locus_tag="VGB85_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0132998.1"
                     /translation="RIEASTSIGTPAPGFVAPVMTGVPVAAHAEPRPAEPAPAEPVAA
                     VEPAAAVQPVAEPAPVFVPPAEPAGVAVFVPEPKPQPRASERGGPRVRAVAGEIGETA
                     ALATAATAEVPIPEVPASEWSPPAAPRVSQAPSTGPAPAPRRDPGASRLRLVVVQRDG
                     SEGEVVPLGDAPLTIGRWQGDLRFPADEFLGSPHARVDPLPTGVRISDLATRNGIFVR
                     ISRPEPVFPGDLFLLGHHLLRLENIPAAGREQDPGPDGVRGFGTPLQPAWGRLVLLGP
                     GGVEAETHYLRHPQVVFGRELGDIVFPADPFVSWQHAQLSVEVAAEAMRVTLTDLRSA
                     NGTYLRARGDVILDPGDMFRVGDQIFRVRMH"
BASE COUNT          375 a         1116 c         1038 g          341 t
ORIGIN      
        1 gccagcggaa cctcggcgcg ccagcccagg cgatctgccg cccgcccaca gtcggcgagc
       61 gagtgggcga cgtccccggc gcgcgccgcc ccgaacacca ccgggctcgt gctgccggtc
      121 accgcgacga tctccgccgc cagcgccgcg atcgtgtggc tgtgtccgct gccgaggttg
      181 gcgaccagcc cgtcgatccc cggcgcgtcg atcgccgcca cgaaggcgcg cgcgacgtcc
      241 tcgacgtaca cgaagtctcg cgtctgcgcg ccgtcgccga gcaccaccag cggctcgccc
      301 gcggtcgccc gcgccaggaa ctgcgacacc accccgctgt acgcgctgcg cgggtcctgc
      361 cgcgggccgt acacgttgaa cgggcgcagc gcggccgtct cgaccccgag ggccgggccg
      421 aggcacgcga agaacagctc ggccgcgcgc ttgctcgccc cgtagggcga ggctggccga
      481 tcgcacatgt cctcggagac aggcacgcgc gccggctcgc cgtagaccgc cgccgacgag
      541 gcgagcacca cccgccgcgc ccccgccccg gcggcccact gcagcaggcg cacgctggcg
      601 cggaggttga ggtcgaggtc ggcgatcggg tcctcgaccg agcgcggcac cgacacctgg
      661 gccccgaggt gcacgaccgc cgccgggcgc accccgccga gcgccgcggc gaagtcgtcg
      721 cacaggtcgg cgtcgatcac ccgcaggtgg cggctgttcg cgtgcgcggc gaggttctcg
      781 cgcttgcccg ccgcgaagtt gtcgacgacc accacctcgc ggccctgcgc gagcaggcgg
      841 tcgaccacgt gcgagccgat gaagccggcg ccgccggtga cgagcacgat ctccgaggcg
      901 acacccatca caacctcaga tcgagtgctg cacgcgcagc atcgcgactt cctcgcggcc
      961 ctgcggcagc tcgagcccgt cgccggtgaa gcgggcgatc gccacggtca cgttgtcggg
     1021 cccgccggcg gcgcaggccg cctcggtgag cgcccggcag cagcgcacgg ggtcctggta
     1081 gcgcagcatc agctcgagga tcatgtcgtc cgcgatcggc ccggtcagcc cgtcgctgca
     1141 cagcagcagc acgtcgccgg cgcgcagctg ggcctgcacg atctcgggac cgacgcgcgg
     1201 ctggaccccg agcgcctgca gcagcacgtt gcggtgctcg tagttgcggg cctggtccgg
     1261 ctgcagccgg cccgccgcga tcagctggcc gacgatgctg tggtcggtcg tcagctgggt
     1321 cagcgcccgg ccgcggcgca ggtaggcccg cgagtcgccg acgtggccga tcagcagttg
     1381 cgccccgatc gccagcgcgg cggtcatcgt cgtgcccatg ccgtggcgca tcgggttgtc
     1441 ggccgcggcc ccgcggatcg cgttgttgac cgccgtgacc gccgcctgca tcgcgatctc
     1501 ggccgcggcc atgtcggcga acgggctgta ctgctgcagc agcttggcca gcgtgtcggc
     1561 ggcgatgttc gacgcgacat cgccggccgc cgccccgccc atgccgtcgc acacggcgac
     1621 cagcgtgccc gccagcgtca gcggctggac gcgggccgac ggctgcaagc cgagctcacc
     1681 ggtcgccagg ttggcgacca cgaagtggtc ctcgttgcgc ttgcggacgc aaccaacctc
     1741 cgtgcgcgcg aagacctcga cctgcgccac ctagtgcatc cttacgcgaa agatctggtc
     1801 gcccacgcgg aacatgtcgc cgggatccag gatgacatcc ccgcgggcgc gaaggtaggt
     1861 tccgttggcc gagcgcaggt cggtgagcgt cacacgcatc gcctccgctg ccacctccac
     1921 gctcagctgc gcgtgctgcc agctgacgaa ggggtccgca gggaagacga tgtcgccgag
     1981 ctcgcgcccg aacaccacct gcgggtggcg gaggtagtgc gtctcggcct cgacgccgcc
     2041 gggcccgagc aagaccaggc ggccccacgc cggctgcagc ggcgtaccga agccgcgcac
     2101 cccgtcgggg ccggggtctt gctcccggcc ggcggccggg atgttctcca ggcgcagcag
     2161 gtgatgcccg agcaggaaca ggtcgcccgg gaacaccggc tcgggcctgg agatccgcac
     2221 gaagatccca ttgcgggtcg ccaggtcgga gatccttacc cccgtcggca gcgggtcgac
     2281 ccgcgcgtgc ggcgagccca ggaactcgtc cgcggggaag cgcaggtcgc cctgccagcg
     2341 gccgatcgtc agcggggcgt cgccgagcgg gaccacctcg ccctcggagc cgtcgcgctg
     2401 gacgacgacc aggcgcagtc gactcgcccc gggatcgcgg cgcggcgccg gggccggccc
     2461 ggtgctcggc gcctgcgaca cccgcggcgc ggctggcggc gaccactcgc tcgccggcac
     2521 ctccgggatc ggcacctccg ccgtcgccgc cgtcgcaagc gccgcggtct caccgatctc
     2581 gccggcgacc gcacgcaccc gcgggccgcc gcgctcggac gccctgggct gcggcttggg
     2641 ctccggcacg aacaccgcga cgcccgcggg ctcggccggc ggcacgaaca ccggcgccgg
     2701 ctcggcgacg ggctgcaccg ccgcggcggg ctcgaccgcc gccaccggct cggcaggcgc
     2761 cggctcggcg ggtcgcggct cggcgtgggc cgcgaccggc acgccggtca tcaccggggc
     2821 gacgaagccc ggcgcgggcg tgccgatcga tgtgctcgcc tcgattcgtc
//
LOCUS       DASWNJ010000156         2543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_497097, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000156 DASWNJ010000000
VERSION     DASWNJ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497097"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VGB85_02930"
     CDS             complement(<1..568)
                     /locus_tag="VGB85_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0132999.1"
                     /translation="MSSLDRIWARPGIEHFKLDWNEASVPPSPRVYAAIVSFLSNSNH
                     LNWYPELGSHSLAHSLADYTGVPAGQILVTNGSDDALELVCKAFIDAEDTVLVPQPTY
                     THFLVYVQSRGATVVDFFADDIFDADIDRLEAVVRTGGFKLVYLVSPNNPTGVVYTRA
                     QVERLLIAAPATLFIVDEAYHEFYGETVV"
     gene            832..1959
                     /locus_tag="VGB85_02935"
     CDS             832..1959
                     /locus_tag="VGB85_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY0133000.1"
                     /translation="MSPAAARSVRVVVIMAGGGGTRLWPASTPTRPKQLLQLSERAPC
                     TLLAAAVERARRLVPLERIYVVTSIALAPAVQVAAPEVPPANIISEPRPRSTAPCVAL
                     ALLHIRQHLLDQGLGEEQVAKTTLTVLPADHHIGDPDAFTALLELACEHAEEHGGIVT
                     LGVEPRKASTAYGYIERGPEPLARRGAAVVYPALRFVEKPDAVRAHIFVETGSFLWNA
                     GIFVMPLGRVAREFERHARLLWAALAPVARALAAHVDPWPTAVTAYEKITPVPIDIAV
                     MEKQRDLCVIPMRLPWTDLGSWQALHEALPKDNHDNVVVAPATAGITLVDSEGVLVWS
                     EGLEVAVLGMRGVAVVISGGRVLVCPLDRAEEVRRLGERRS"
     gene            1995..>2543
                     /locus_tag="VGB85_02940"
     CDS             1995..>2543
                     /locus_tag="VGB85_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY0133001.1"
                     /translation="MTTRDEAPPTPVRPAHEAEPWSYARTQKDPAVDELIDLYFHRPI
                     AYALLRRFEHAAWRPTPNQFTLTGGAIGVLAAAVVMVAAPGSLLWPVAAFMMFFANIL
                     DCCDGMIARLTGQAGTRGMLLDGMIDFIVGVSFWLAMSIRTAPDWGLGTPLAALAIIL
                     SILIHTGLYDHIRIRFGVLVNPP"
BASE COUNT          360 a          849 c          939 g          395 t
ORIGIN      
        1 cgacgacggt ctcgccgtag aactcgtggt aggcctcgtc gacgatgaac agggtcgccg
       61 gggcggcgat cagcaggcgc tcgacctgcg cgcgcgtgta gacgacgccg gtcgggttgt
      121 tggggctgac gaggtagacg agcttgaagc cgccggtgcg cacgacggcc tcgaggcggt
      181 cgatgtcggc gtcgaagatg tcgtcggcga agaagtcgac gacggtggcg ccgcgcgact
      241 gcacgtacac caggaagtgc gtgtaggtcg gctgcgggac gaggacggtg tcctcggcgt
      301 cgatgaaggc cttgcacacc agctcgaggg cgtcgtccga gccgttggtg acgaggatct
      361 gcccggcggg cacgcccgtg tagtctgcca gcgagtgggc cagcgagtgg ctgccgagct
      421 cggggtacca gttgagatgg ttgctgttgc tcaggaacga gacgatcgcc gcatacaccc
      481 gcggcgaggg ggggacgctc gcctcgttcc agtcgagctt gaagtgctcg atccccggac
      541 gcgcccagat gcggtccagc gaagacacgg ggctgtacgg cgccagatgc ttgagcgagg
      601 ggtggggacg caaagcaggc tgagtttgcg cgaacgtagg ggtctgtcaa gactttgtgg
      661 cgtgtgcgtg acctgcgtgt caccccatga atacgcggtt ctgggagctt tccgtgtgaa
      721 atggtcagcg caccgacggg ccctcacggg agcatcgggc ccccgggggt cgtttcaggg
      781 gccagcgcgc gtctggtatg ccggcccctg tccgggtacc ctgcgtggcc catgtctcca
      841 gcggcagccc gcagcgtcag ggtcgtcgtc atcatggcgg gcggcggcgg gacgcgtctg
      901 tggccggcgt cgacgccgac ccggcccaag cagctgctgc agctgagcga gcgggcgccg
      961 tgcactttgc tcgccgcggc cgtcgagcgc gcgcgccggc tggtgccgct cgagcgcatc
     1021 tacgtggtga cgagcatcgc gctggcgccg gcggtgcagg tggccgcgcc cgaggtcccg
     1081 ccggcgaaca tcatcagcga gccgcgcccg cgcagcacgg cgccgtgcgt cgcgctggcg
     1141 ctgctgcaca tccgccagca cctgctcgat cagggcctcg gcgaggagca ggtcgcgaag
     1201 acgacgctga cggtgctgcc cgccgatcat cacatcggcg atcccgatgc attcacggcg
     1261 ctgctcgagc tggcctgcga gcacgccgag gagcacggtg ggatcgtcac gctgggggtc
     1321 gagccgcgca aggcgagcac cgcgtacggc tacatcgagc gcgggccgga gccgctggcg
     1381 cgacgcggcg cggcggtcgt gtatccggcg ctgcgcttcg tcgagaagcc ggacgccgtg
     1441 cgcgcccaca tcttcgtcga gaccggctcg ttcctgtgga acgcggggat cttcgtgatg
     1501 ccgctcgggc gcgtcgcccg ggagttcgag cgccacgcca ggctgctgtg ggcggcgctt
     1561 gcgccggtcg ccagggccct cgcggcgcac gtcgacccgt ggccgacggc ggtcacggcc
     1621 tacgagaaga tcaccccggt gccgatcgat atcgccgtga tggagaagca gcgcgacctg
     1681 tgcgtgatcc cgatgcgcct gccatggacc gacctcggct cgtggcaggc gctgcacgag
     1741 gcgctgccca aggacaacca cgacaacgtg gtcgtcgcgc cggcgaccgc gggcatcacg
     1801 ctggtcgaca gcgagggcgt gctggtgtgg agcgagggcc tcgaggtcgc cgtgctcggc
     1861 atgcgcgggg tcgcggtcgt gatcagcggc gggcgcgtgc tcgtctgtcc gctcgaccgc
     1921 gccgaggagg tccggcggct cggcgagcgg cgatcttgac agtgtcgctg catctttgcg
     1981 acggtcaaag ggccatgacg acgcgcgacg aagctcctcc gacccccgtc cgccccgcac
     2041 acgaggccga gccgtggagc tacgcgcgca cgcaaaagga ccccgcggtc gacgagctga
     2101 tcgacctcta cttccaccgg ccgatcgcgt acgcgctgct gcgtcggttc gagcacgcgg
     2161 cgtggcgacc gacgccgaac cagttcacgc tcaccggcgg tgcgatcggc gtgctcgcgg
     2221 cggcggtcgt gatggtggcc gcgcccggct cgctgctgtg gccggtcgcc gcgttcatga
     2281 tgttcttcgc caacatcctc gactgctgcg acgggatgat cgcgcgcctg accggccagg
     2341 cgggcacccg cgggatgctg ctcgacggca tgatcgactt catcgtcggc gtgtcgttct
     2401 ggctggcgat gtcgatccgc accgcccccg actggggcct cggcacgccc ctcgcggcgc
     2461 tggcgatcat cctcagcatc ctgatccaca ccggcctcta cgatcacatc cggatccgct
     2521 tcggcgtgct cgtcaacccg ccg
//
LOCUS       DASWNJ010000157         1660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_639422, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000157 DASWNJ010000000
VERSION     DASWNJ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639422"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1439
                     /locus_tag="VGB85_02945"
     CDS             <1..1439
                     /locus_tag="VGB85_02945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0133002.1"
                     /translation="VLGRRRAELLGRTDREVLPTSVAELFAASESQVLSRGVPVECEE
                     TLPGGAEGPRSFFSVKFPLRRADGTIVAMCGISTDITRRRQAEAALVDLNESLERRIA
                     ERTQAAEEASRAKSEFLARMSHELRTPLNAILGYSELIEDEMRDRGEEGYTSDVRRIR
                     AAGKHLLQLINDILDISKIEAGKMLVHVEEFSVAALVDEVLAAVRPGIERNRNALRLE
                     LDPAAGMMRSDSLKVRQCLLNLLSNAGKFTHNGAVTLSVKRQLVNGRAWLLFAVSDTG
                     IGISPEHVGQLFREFYQVDTSSTRRYEGTGLGLVITRRLCHMLGGEIRVFSKPGGGST
                     FTAELPAVHIEAGAPEIALELEVLAERDVARARMSVRELCQKLGARPLSLQKIATVVS
                     ELARNMVLYAGGGRLVVGSVGTEQKCVFIRAIDEGSGIPELEAILAGRKVSRNGRGLG
                     LVGAKRLADGFRIHTGPTGTWIEVEVNL"
     gene            1436..>1660
                     /locus_tag="VGB85_02950"
     CDS             1436..>1660
                     /locus_tag="VGB85_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133003.1"
                     /translation="MNDPVREPDDRALLAVLGRYVSPTRAQSILVRARRDMPGLAPRG
                     PAPSTQLLSRLAEGLRMFIGEADTRAAVAEL"
BASE COUNT          234 a          573 c          615 g          238 t
ORIGIN      
        1 tggtgctcgg ccggcggcgc gccgagctgc tcggtcgcac cgaccgcgag gtgctgccga
       61 cgagcgtcgc cgagctgttc gccgcgagcg agtcgcaggt gctgtcgcgc ggggtcccgg
      121 tcgagtgcga ggagacgctg ccgggcggcg ccgaggggcc gcggtcgttc ttctcggtca
      181 agttcccgct gcgtcgggcc gacgggacga tcgtcgcgat gtgcgggatc tcgaccgaca
      241 tcacgcgccg gcgtcaggcc gaggcggcgc tcgtcgacct caacgagtcg ctcgagcgcc
      301 ggatcgccga gcgcacgcag gcggccgagg aggcctcgcg ggccaagagc gagttcctgg
      361 cgcgcatgag ccacgagctg cgcacgccgc tcaacgcgat cctcggctac agcgagctga
      421 tcgaggacga gatgcgcgac cgcggcgagg aggggtacac cagcgatgtc cgccgcatcc
      481 gcgccgccgg caagcacctg ctgcagctga tcaacgacat cctcgacatc tcgaagatcg
      541 aggccggcaa gatgctggtc cacgtcgagg agttctcggt cgccgcgctc gtcgacgagg
      601 tgctcgccgc ggtccgcccc ggcatcgagc gcaaccgcaa cgcgctgcgc ttagagctcg
      661 acccggcggc gggcatgatg cgctccgact cgctcaaggt ccggcagtgt ttgctcaacc
      721 tgctcagtaa tgcgggcaag ttcacgcaca acggcgcggt gacgctgtcg gtcaagcgcc
      781 agctggtcaa cggtcgcgcg tggctgctgt tcgcggtcag cgacacgggc atcggcatct
      841 cgccggagca cgtcgggcag ctgttccgcg agttctatca ggtcgacacc tcgtcgacgc
      901 gccgctacga gggcaccggc ctcggcctgg tgatcacgcg gcgcctgtgt cacatgctcg
      961 gcggcgagat ccgggtgttt agcaagccgg gcggcgggtc gaccttcacg gccgagctgc
     1021 cggcggtgca catcgaggcg ggcgccccgg agatcgcgct cgagctcgag gtgctggccg
     1081 agcgcgacgt ggcccgcgcg cgcatgtcgg tccgcgagct gtgccaaaag ctcggcgcgc
     1141 gcccgctgtc gctgcagaag atcgccaccg tcgtcagcga gctggcccgc aacatggtgc
     1201 tgtacgcggg cggcgggcgg ctggtggtcg gctcggtcgg caccgagcaa aagtgcgtgt
     1261 tcatccgcgc gatcgacgag ggctcgggca tccccgagct cgaggcgatc ctcgcggggc
     1321 gcaaggtcag ccgtaacggt cgaggcctcg gcctcgtcgg cgccaagcgc ctggccgacg
     1381 gctttcggat ccacaccggc ccgaccggta catggatcga ggtcgaggtg aacctgtgaa
     1441 cgatcccgtg cgcgagcccg acgatcgggc gctgctggcc gtgctcgggc gctacgtcag
     1501 tccgacgcgc gcgcagagca tcctggtgcg cgcgcgccgg gacatgcccg ggctcgcgcc
     1561 gcgcgggccg gccccgtcga cgcagctgct ctcgcggctc gccgagggcc tgcgcatgtt
     1621 catcggcgag gccgacacgc gcgccgcggt ggccgagctg
//
LOCUS       DASWNJ010000158         2002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_639472, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000158 DASWNJ010000000
VERSION     DASWNJ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639472"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1951)
                     /locus_tag="VGB85_02955"
     CDS             complement(<1..1951)
                     /locus_tag="VGB85_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HEY0133004.1"
                     /translation="MSTLEALLPLFEPETLARISSKKAFAAGKELFDVGRTVSDIVFD
                     SGCLRGKVKGSHPLPHNTSLKLLPDGTLEANCTCPTYTDGWERICQHAVTLALTLRKQ
                     YQSGGEITRTQNPWVQDLASGNAANLQRYQVDVRKGHWHVMVFKGGGTPIISRKRSEG
                     MSPADRLIMHFLEQEVDDSDDGGHIIDDIAFAGLLYFARNSTVGLKGVGKLQFGPEPL
                     ILRIRAEARTTDASVELHAYLEQRSNGRTIEVNSGRVIVGAPTWFLLPDTAEVFQVPD
                     TPPWVLEAVARQPRIVMDSRVSAQQMDALSETLHSVGVPQQDLFALASDSRPIDRIVA
                     SVEADANKVKIALAARYGNVTLAIAGNDPESPRYSINLGDQTLTFYRDMSGEAAARKV
                     LTDLGLRWSPNDDGFTAEGDAGVEFIIVGLPLLPDTWERRIPVLPKIRSKSPTPKISV
                     HNKEASVLDLSAVVDVDGQEDLISFRDLLRWLHEGRRWVTLADGSVAKLDPQILQPIA
                     EAAGAIDFDKGGHAELSTLELGTLSRLLGAAPSADVAKEVKKLMENMTGDKSSKAPRK
                     AKALTAKLREYQRSGFAWLWQLHENNMTGILADDMGLGKTVQALALLTKAKEEEGDVP
                     SLIVCPTSVLSVWRQEVKKWAPTLSV"
BASE COUNT          280 a          672 c          701 g          349 t
ORIGIN      
        1 tcaccgacag cgtcggcgcc cacttcttga cctcctgccg ccacaccgac agcaccgagg
       61 tcgggcacac gatcagcgag ggcacgtcgc cctcctcctc cttggccttc gtcagcagcg
      121 cgagcgcctg gaccgtcttg ccgaggccca tgtcgtcggc caggatgccg gtcatgttgt
      181 tctcgtggag ctgccacaac cacgcgaagc ccgagcgctg atactcgcgc agcttggcgg
      241 tcagcgcctt ggccttgcgc ggcgccttcg agctcttgtc gccggtcatg ttctccatga
      301 gcttcttgac ctccttggcg acgtccgcgc tgggcgcggc gccgaggagg cggctcaggg
      361 tcccgagctc gagcgtcgac agctcggcgt gaccaccctt gtcgaaatcg atcgcgccgg
      421 cggcctcggc gatcggctgg aggatctgcg ggtcgagctt ggcgaccgag ccgtcggcga
      481 gcgtgaccca gcggcggccc tcgtgcagcc agcgcagcag gtcgcggaac gagatcaggt
      541 cctcctgacc gtcgacgtcg accaccgcgc tgaggtcgag cacgctcgcc tccttgttgt
      601 gcaccgagat cttcggcgtc ggcgacttgc tccggatctt cggcagcacg gggatccttc
      661 gctcccacgt gtccggcagc agcggcaggc cgacgatgat gaactcgacg cccgcgtcgc
      721 cctcggcggt gaagccgtcg tcgttggggc tccagcgcag gccgaggtcg gtcagcacct
      781 tgcgcgccgc ggcctcgccg gacatgtcac gatagaacgt gagggtctgg tcgccgaggt
      841 tgatcgagta gcgcggcgac tccgggtcgt tgccggcgat cgccagggtg acgttgccgt
      901 agcgggccgc cagcgcgatc ttcaccttgt tggcgtcggc ctcgacgctg gcgacgatgc
      961 ggtcgatcgg ccgcgagtcc gacgcgagcg cgaacaggtc ctgctgcggc acgccgaccg
     1021 agtgcagcgt ctccgacagc gcgtccatct gctgcgccga cacgcgcgag tccatgacga
     1081 tgcgcggctg acgggcgacc gcctcgagca cccacggcgg cgtgtcgggg acctggaaca
     1141 cctcggcggt gtcgggcagc aggaaccacg tcggcgcgcc gacgatcacg cggccgctgt
     1201 tgacctcgat cgttcggccg ttggagcgct gctcgaggta cgcgtgcagc tcgacgctgg
     1261 cgtcggtggt gcgcgcctcg gcgcggatgc gcaggatcag cggctcgggc ccgaattgca
     1321 gcttgccgac gcccttgagg ccgaccgtgc tgttgcgcgc gaagtacagc aggcccgcga
     1381 acgcgatgtc gtcgatgatg tggccgccgt cgtcgctgtc gtcgacctcc tgctcgagga
     1441 agtgcatgat caggcggtcg gcggggctca tgccctctga ccgcttgcgg ctaataatag
     1501 gcgtgccgcc gcccttgaac accatcacgt gccagtggcc cttgcggacg tcgacctggt
     1561 agcgctgtaa attggccgcg tttccgctgg cgaggtcctg gacccacggg ttctgcgtgc
     1621 gcgtgatctc gccgccggac tggtattgct tgcgcagcgt cagcgcgagc gtcaccgcgt
     1681 gctggcagat ccgctcccag ccgtcggtgt aggtcgggca cgtgcagttg gcctcgagcg
     1741 tgccgtccgg gaggagcttc aggctggtgt tgtgcggcag cgggtgcgag cccttgacct
     1801 tgccgcgcag gcagccgctg tcgaacacga tgtcggagac cgtccggccg acgtcgaaca
     1861 gctctttacc ggccgcgaac gccttcttgc tggagatgcg ggcgagcgtc tccggctcga
     1921 acagaggtaa caacgcttcg agtgtggaca cgcgatttcc tgcgagaggg gatcgggcgg
     1981 ggctcatgaa ggagccctgc gc
//
LOCUS       DASWNJ010000159         2510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_109280, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000159 DASWNJ010000000
VERSION     DASWNJ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109280"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VGB85_02960"
     CDS             <1..558
                     /locus_tag="VGB85_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEY0133005.1"
                     /translation="RDLESLRTAELPLVAERGRGGGFALERDYSMPPVNFTAREAAIL
                     ISAAKFLAAMRVLPFAETLATASDKLRAALSRSAQREFLKRLDELQFVGVPAKATLPE
                     VRAALEQAWLLQRPLWLRYAGSRGESERTVRLETIVMERGETLLNVVDLAHGEARQLR
                     LDRVLAARVEAEEQPPAPAKRRRAE"
     gene            complement(562..1317)
                     /locus_tag="VGB85_02965"
     CDS             complement(562..1317)
                     /locus_tag="VGB85_02965"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY0133006.1"
                     /translation="MPTYPRTAPGPFRADQIRDGDEYELSDGHAVRCLPSGSRHGNAQ
                     LDGGRVLASDPAVRGRAGIDIGIAWNDDKNLRAPDLVVGHSERAPGWLRSVPPLAVEY
                     ADTGQDEAELAAKISELHAAGTKYIWVVRLTGPLRVDVHTRGEPIRTVGADGELLAPG
                     VLQNPVPVRALVDANAADAASLRNLLQAHGYTSIEEIQAEALARAILTVLATRGISVD
                     DEQAGRIRGCRDMPTFARWLRLAVTASSVGELD"
     gene            complement(1366..2037)
                     /locus_tag="VGB85_02970"
     CDS             complement(1366..2037)
                     /locus_tag="VGB85_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133007.1"
                     /translation="MLSRADFVARHPRLYHIAAGGSWPSLAERGLLSTSALLDLFEVD
                     AVTRRALERECRVKQETLEHPVHGRVVLRDQRPMLPERLGPCLRGGITPEDWYHEINR
                     RVFFWPTDEDRNTFLNARAYRKQPKLLLTIDTAALLERHAARVELSRINSGSTHPMPS
                     PRGLDDFITLDRYLSEGGYRGHSPRTHFRELTVLGGVPDLAAVVLRAEEYLPGEAPRT
                     LHTQS"
     gene            complement(2101..>2510)
                     /locus_tag="VGB85_02975"
     CDS             complement(2101..>2510)
                     /locus_tag="VGB85_02975"
                     /inference="COORDINATES: protein motif:HMM:NF024216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0133008.1"
                     /translation="VALAEEFKRTIADARTVALPTVWLEQFELAVRTIADLTVGLAAR
                     GQAGEHEFMLRHSSDYLDLFSTIVLTWQWLRQAIVAARALAAANREDTDFYEGKLCAA
                     QYWFATELPRIDHLVRLCREGEDSYARMQPAWF"
BASE COUNT          376 a          822 c          957 g          355 t
ORIGIN      
        1 cgcgacctcg agagcctgcg gacggccgag ctgccgctgg tggccgagcg cgggcgcggc
       61 ggaggcttcg cgctcgagcg cgactactcg atgccgccgg tcaacttcac cgcccgcgag
      121 gcggcgatcc tgatctcggc ggcgaagttc ctggcggcga tgcgcgtgct gccgttcgcg
      181 gagacgctgg cgaccgcgag cgataaattg cgcgcggcac tgtcacgctc ggcccagcgg
      241 gaatttctca aacggctcga cgagctgcaa ttcgtcggcg tgccggcgaa ggcgaccctg
      301 ccggaggtcc gcgcggcgct cgagcaggcg tggctgctgc agcgaccgct gtggctgcgc
      361 tacgccggca gccgcggcga gagcgaacga accgtgcgcc tcgagacgat cgtgatggag
      421 cgcggcgaga cgctgctcaa cgtcgttgac ctcgcgcacg gcgaggcgag acagctgcgc
      481 ctcgatcgcg tgctcgcggc ccgcgtcgag gcggaggagc aaccaccggc gcccgcgaag
      541 cgccgccgcg cggagtgagc atcagtcgag ctcgccgacg gacgaggccg tgacggcgag
      601 acgcaaccag cgggcaaagg tcggcatgtc gcggcagccg cggatgcgcc cggcctgctc
      661 gtcgtcgacg gagatcccgc gggtggcgag cacggtgagg atcgcccgcg ccagtgcctc
      721 ggcctggatc tcctcgatgc tggtatagcc gtgcgcctgg agcaggttac gcaggctggc
      781 ggcgtcggcg gcgttggcgt cgacgagggc gcgcaccgga acgggattct gcaggacgcc
      841 cggggcgagc agctcgccat ccgcgccgac ggtgcgtatc ggttctccac gggtgtggac
      901 atcgacgcgc agcgggccgg tcaggcgcac gacccagatg tacttcgtgc cggcggcgtg
      961 caactcggag atcttggcgg ccagctcggc ctcgtcctgg ccggtgtcgg cgtactcgac
     1021 ggccagcggc ggcacgctgc gcaaccatcc cggcgcgcgc tcggagtggc cgaccaccag
     1081 gtcgggcgcg cgcaggttct tgtcgtcgtt ccaggcgatg ccgatgtcga tgcccgcgcg
     1141 gccgcgaacg gcggggtcac tggcgaggac gcggccgccg tcgagctggg cgttgccatg
     1201 gcgggagccc gagggcaagc agcggaccgc gtggccgtcg ctgagctcgt actcgtcgcc
     1261 gtcacggatc tggtcggccc ggaacgggcc aggggcggtc ctcgggtacg tcggcatgcc
     1321 cgcacgatag cacgcccgcg cgcccgggac agccgcccac gggtctcaac tctgggtgtg
     1381 gagcgtccgc ggggcctccc cggggagata ctcctcggcg cgcaatacga cggctgcgag
     1441 gtcgggcacg ccgccgagga cagtcagctc gcgaaagtgc gtgcgtggcg agtgcccgcg
     1501 atagccacct tcggagaggt agcggtccag agtaatgaag tcgtccaggc cgcgggggct
     1561 cggcatcgga tgtgtgctgc cgctgttgat ccgcgatagc tcgacccgcg ccgcgtggcg
     1621 ttcgagcaag gcggcggtgt cgatcgtgag cagcagcttg ggttgcttgc ggtaggcgcg
     1681 agcgttgagg aaggtgttgc ggtcctcatc ggtcggccaa aaaaatacgc ggcgattgat
     1741 ctcgtggtac cagtcctcgg gcgtgatgcc accgcgcagg cacgggccca agcgctcggg
     1801 tagcatcggt cgctggtcac gcagcaccac gcgaccatgg accggatgct cgagcgtctc
     1861 ctgcttgacc cggcattcac gctcgagagc tcgacgagtc acggcgtcga cctcgaagag
     1921 gtcaagcagg gccgatgtgc tgagcaggcc gcgttcggcg agggagggcc aggagccacc
     1981 ggccgcgatg tggtagaggc gggggtggcg agcgacgaag tcggcgcggg agagcatcgg
     2041 tggcgacctt atcagccgcc gcgtcgtggc tcacgcgcac acagtgcgcg cgagtgcgcc
     2101 tcagaaccac gccggctgca tgcgggcgta ggagtcctcg ccctcgcgac acaggcgcac
     2161 gaggtggtcg atgcgcggca gctcggtggc gaaccagtac tgggcggcgc acagcttgcc
     2221 ctcataaaaa tcggtgtcct cgcggttggc ggccgcgagc gcgcgcgcgg cgacgatcgc
     2281 ctggcgcagc cactgccagg tgagcacgat cgtcgagaac aggtcgaggt agtcgctgct
     2341 gtggcgcagc atgaactcgt gctcgccggc ctggccgcgc gcggcgagcc cgacggtcag
     2401 gtcggcgatc gttcgcacgg cgagctcgaa ctgctcgagc cacacggtcg gcagcgcgac
     2461 ggtgcgggcg tcggcgatcg tgcgcttgaa ctcctccgcg agcgccacga
//
LOCUS       DASWNJ010000160         2536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_427031, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000160 DASWNJ010000000
VERSION     DASWNJ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427031"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="VGB85_02980"
     CDS             complement(<1..1010)
                     /locus_tag="VGB85_02980"
                     /inference="COORDINATES: protein motif:HMM:NF036965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY0133009.1"
                     /translation="MTGGRSGDMTALPDLPAPDPPAPDRVLIRFEPDGARPKEVRVPG
                     GATLFDAASWNGIAVDSTCGGHGTCRKCLMRVVEGDVAVSRLDPRAFSPDELRAGWRL
                     ACRARAEQDLRVEVPPLVTRPKAATVGVGRQVILRPALQKRCVTLPEATLSDQRTDVE
                     RLRDALPDLELRVDLPAIRDLSRAMRASSDSGDGVEQPVTAVVVDDVLVAVEPGDTTA
                     RLFGIAFDLGTTTVVATLLDLATGTPVAVASLLNPQQPYGADVITRISATMMDPDSLG
                     RLTALAHEGLDQLVGEVCADGGVERREVYEIALAGNATMVHLALGIPPEPLGVAPFVM
                     ASR"
     gene            complement(1007..1924)
                     /locus_tag="VGB85_02985"
     CDS             complement(1007..1924)
                     /locus_tag="VGB85_02985"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF005719.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY0133010.1"
                     /translation="MPEIAHTVVRSATREVTIGSDQPFCLIGERLNPTGRKIFAEALR
                     RGDLSRIAIDVEQQVAGGATMLDINMGVPLADEAELLAKAVTMVQELTDLPLCIDSSI
                     VEALEAGLEAYNGKALVNSITGERERMDQILPIVKRHGAAVIALPNEEDEIPDEPERR
                     LEIARKILDVAVGEYGIPLEDIVFDPLAMPVGADTSLVKTTLRTIELIHDELGLNMTL
                     GAGNVSFGMPGRSALGAAFLPMAMQCGLTSAIMDVRSPQMVEAVKAADLLLANDDWGA
                     AWIARSRAAAAAAASPPSSPRLPAATPRT"
     gene            complement(1929..>2536)
                     /locus_tag="VGB85_02990"
     CDS             complement(1929..>2536)
                     /locus_tag="VGB85_02990"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HEY0133011.1"
                     /translation="RVLELTREGLDTGLSPESMLFDALIPSLEEVGARFERGDFFVPE
                     MLVAGKAMAGALDILRPLLAETGAKTVGTFLMSTVKGDVHDIGKNLVNIMLEGAGFHV
                     IDLGVQVSPEKVIAGIHEHKPDIVGFSAFLTTTMPMFKANINALQKAGLRDQVIVMVG
                     GAPVTQEYADAVGADGYAADAATAVRKAKELLAARRAAVGV"
BASE COUNT          344 a          899 c          931 g          362 t
ORIGIN      
        1 cgcgacgcca tgacgaacgg ggcgacaccc agcggctcgg gcggaatgcc cagcgcgagg
       61 tggaccatcg tggcgttgcc ggccagagcg atctcgtaga cctcgcggcg ctcgacgccg
      121 ccgtcggcac acacctcgcc gacgagctgg tcgaggccct cgtgggccag cgcggtgagc
      181 cggccgagcg agtcggggtc catcatcgtc gcgctgatac gggtgatcac gtcggcgccg
      241 tagggctgct gggggttgag cagcgacgcg acggcgaccg gggtgcccgt cgcgaggtcg
      301 agcagggtcg cgacgacggt cgtggtgccg aggtcgaagg cgatgccgaa caggcgcgcg
      361 gtggtgtcac cgggctcgac ggcgaccagc acgtcgtcga cgaccacggc ggtgacgggc
      421 tgctcgaccc cgtccccgga gtcggacgag gcgcgcatgg cgcggctcag gtcgcggatc
      481 gccggcaggt ccacccgcag ctcgaggtcg ggcagggcgt cgcgcaggcg ttcgacgtcg
      541 gtgcgctggt cggacagcgt ggcctcgggc agcgtcacgc agcgcttctg cagggcgggg
      601 cgcaggatca cctgccggcc gacgccgacc gtggccgcct tcggccgggt gaccagcggc
      661 ggcacctcca cccgcaggtc ctgctcggcc cgggcccggc aggccagccg ccagcccgcg
      721 cgcagctcgt cggggctgaa cgcgcgcggg tcgagccggg acacggccac gtcgccctcg
      781 acgacccgca tcaggcactt gcggcacgtg ccgtgcccgc cgcaggtgga gtcgacggcg
      841 atgccgttcc agctcgcggc gtcgaacagc gtggccccgc cgggcacccg cacctctttc
      901 gggcgggccc cgtcgggctc gaagcggatg aggacccggt ccggcgcggg agggtccggc
      961 gcgggcaggt ccggcagggc ggtcatgtcc ccgctgcggc ccccagtcat gtccgaggag
     1021 tcgccgccgg caggcgggga ctgctcggtg gcgacgctgc cgccgcggcc gccgcccggg
     1081 accgcgcgat ccaggccgcg ccccagtcgt cgttggcgag cagcaggtcg gcggccttga
     1141 cggcctcgac catctgcggg ctgcgcacgt ccatgatcgc gctggtcagg ccgcactgca
     1201 tggccatcgg caggaacgcc gcgcccagcg cggagcgccc gggcatgccg aacgagacgt
     1261 tgccggcgcc gagggtcatg ttcagcccga gctcgtcgtg gatcagctcg atggtgcgca
     1321 gcgtggtctt caccagcgag gtgtccgcgc ccaccggcat cgccagcggg tcgaacacga
     1381 tgtcctccag cgggatcccg tactcgccga ccgcgacgtc gaggatcttc ctcgcgatct
     1441 ccagacggcg ctccggctcg tccgggatct cgtcctcctc gttgggcagg gcgatcaccg
     1501 ccgcgccgtg ccgcttgacg atcgggagga tctggtccat ccgctcccgc tcgcccgtga
     1561 tggagttgac cagcgccttg ccgttgtacg cctcgagccc ggcctccagc gcctcgacga
     1621 tcgaggagtc gatgcacagc ggcaggtcgg tgagctcctg caccatcgtg acggccttgg
     1681 cgagcagctc ggcctcgtcg gcgagcggga cgcccatgtt gatgtcgagc atcgtcgccc
     1741 cgccggcgac ctgctgctcg acgtcgatgg cgatgcgcga gaggtcaccg cggcgcagcg
     1801 cctcggcgaa gatcttgcgg ccggtcgggt tgagccgctc cccgatcagg cagaagggct
     1861 ggtccgaccc gatggtcacc tcgcgggtgg ccgagcggac gacggtatgt gcgatctcgg
     1921 gcatcgggtc agacccccac agcggcacgg cgagcggcga gcagttcctt ggccttgcgg
     1981 acggcggtcg cggcatccgc ggcgtacccg tcggccccca cggcgtcggc gtactcctgg
     2041 gtgaccgggg cgcccccgac catcacgatc acctggtcgc gcaggcccgc cttctgcagc
     2101 gcgttgatgt tggccttgaa catcggcatc gtggtggtga ggaacgcgga gaagccgacg
     2161 atgtccggct tgtgctcgtg gatcccggcg atcaccttct ccggcgacac ctgcacgccg
     2221 aggtcgatca cgtggaagcc tgcgccctcg agcatgatgt tcacgaggtt cttgccgatg
     2281 tcgtgcacgt cgcccttgac ggtgctcatg aggaacgtgc cgacggtctt cgcgccggtc
     2341 tcggcgagca gcgggcgcag gatgtccagc gcgccggcca tcgccttgcc cgcgacgagc
     2401 atctccggga cgaagaagtc gccgcgctcg aagcgggccc cgacctcctc caacgagggg
     2461 atcagcgcgt cgaacagcat cgactcggga ctcaggcccg tgtcgagacc ctcccgggtc
     2521 agttccagca cgcgtg
//
LOCUS       DASWNJ010000161         1704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_462585, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000161 DASWNJ010000000
VERSION     DASWNJ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462585"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1704)
                     /gene="secF"
                     /locus_tag="VGB85_02995"
     CDS             complement(<1..>1704)
                     /gene="secF"
                     /locus_tag="VGB85_02995"
                     /inference="COORDINATES: protein motif:HMM:NF009583.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY0133012.1"
                     /translation="ACNPAGDPKRRIAIVLDGDVISSPQVDPSVRCGTGIQGGTTQIT
                     GDFDRDEAQELAVLIKGGALPVPVEVVSRSFVGPTLGQEAIDASVEAGVIGLTLTAIF
                     LVIVYRFVGFLAAVALASYGLISYGALVALGATLTLPGLGGLLLSAGLAIDANVLVFE
                     RAREEYAASRSKRLMPALDNGFAKAFSAIADSNITTLLAAGLLFFLASGPVRGFGVTL
                     VIGVLASVVSALLVTRVLTEFGLRRGLIAKRPAFTGLASLGRFRTWLEAKRPDLLKHT
                     RTYLGITAAVLVLAVLGMLVRGFNFGVEFTGGRVVEFATSQDLSVAEARSAVEQAGFP
                     TADVQETNGENIAVRTTDISEQEVAEIRTALAAQGGEVTVASNELIGPTLGDELRNKA
                     LIALVIALLAQLAYLAFRFRWTMAAGTVLAMLHDIVLVVGIFAWLGKPIDGVFLAAAL
                     TIIGVSVNDSVVTMDRIRETWSLHRSRPLAQVANDAIIATAPRTINTGLGAFFILGAL
                     TVLGGRSLTDFALALLIGLFVGTYSSAFLASPLMLWFEERNSAPPPMPKRTTAPARRS
                     KP"
BASE COUNT          273 a          556 c          659 g          216 t
ORIGIN      
        1 ggcttcgaac gccgagccgg cgccgtggtg cgcttgggca tcggtggtgg cgcgctgttg
       61 cgctcctcga accagagcat cagcggtgag gccaggaagg ccgaggagta cgtcccgacg
      121 aacaggccga tgagcagcgc cagggcgaag tcggtgagcg accggccgcc cagcaccgtc
      181 agcgccccga ggatgaagaa cgcacccaga ccggtgttga tcgtgcgcgg cgccgtggcg
      241 atgatcgcgt cgttggccac ctgcgcgagg gggcggctgc ggtgcagcga ccaggtctcg
      301 cggatgcggt ccatggtgac gacggagtcg ttcaccgaca caccgatgat cgtcagcgcc
      361 gcggcgagga acaccccgtc gatcggcttg ccgagccagg cgaagatccc gaccacgagc
      421 acgatgtcgt ggagcatcgc gagcaccgtg ccggctgcca tcgtccagcg gaaccggaag
      481 gcgaggtagg cgagctgggc gagcagggcg atgacgaggg cgatgagcgc cttgttgcgc
      541 agctcgtcgc cgagcgtcgg gccgatgagc tcgttgctgg cgaccgtcac ctcgccgccc
      601 tgcgccgcca gcgcggtacg gatctctgcg acctcctgct cggagatgtc ggtggtgcgc
      661 actgcgatgt tctcgccgtt cgtctcctgc acgtcggcgg tcgggaagcc ggcctgctcg
      721 acggccgagc gcgcctcggc caccgacagg tcctggctgg tcgcgaactc gaccacgcgg
      781 ccgccggtga actcgacgcc gaagttgaag ccgcgcacca gcatgccgag cacggccagg
      841 acgagcaccg cggcggtgat gccgaggtag gtccgggtgt gcttgagcag gtcggggcgc
      901 ttggcctcga gccaggtgcg gaagcggccc aggctggcga ggccggtgaa ggccgggcgc
      961 ttggcgatga gcccgcgccg gaggccgaac tcggtcagca cccgggtcac cagcagcgcc
     1021 gacaccacgg aggccagcac accgatgacg agcgtgacgc cgaagccgcg gaccgggccg
     1081 gaggccagga agaacagcag gccggcggcg agcagcgtgg tgatgttgga gtcggcgatg
     1141 gcgctgaacg ccttggcgaa gccgttgtcg agcgccggca tgagccgctt gctccgcgag
     1201 gcggcgtact cctcgcgggc gcgctcgaag acgagcacgt tcgcgtcgat cgcgaggccg
     1261 gccgacagca gcagaccgcc gaggccgggg agcgtcagcg tggcgccgag ggcgacgagc
     1321 gcgccgtacg agatcaggcc gtacgacgcc agggcgaccg cggccaggaa gccgacgaag
     1381 cggtagacga tgacgaggaa gatcgcggtg agcgtcaggc cgatgacgcc ggcctcgacg
     1441 ctggcgtcga tggcctcctg gcccagcgtc gggccgacga acgagcggct gaccacctcg
     1501 accggcacgg gcagcgcgcc gcccttgatg aggacggcga gctcctgcgc ctcgtcgcgg
     1561 tcgaagtcgc cggtgatctg cgtcgtgccg ccctggatgc cggtgccgca cctgaccgag
     1621 gggtcgacct ggggcgagct gatgacgtcg ccgtcgagga cgatggcgat ccggcgcttg
     1681 gggtcgccgg ccgggttgca ggcc
//
LOCUS       DASWNJ010000162         2627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_250516, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000162 DASWNJ010000000
VERSION     DASWNJ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250516"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VGB85_03000"
     CDS             complement(<1..459)
                     /locus_tag="VGB85_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0133013.1"
                     /translation="MLRLASADDLPALRELERAAGAPFRAIGMATIADDEPPALAVLA
                     GFQRDGRAWVIDDDAGRPVAYLLLEVVDGCAHVEQMSVHPGHARRGLGRELLGAAEGW
                     ARAAGSPAITLTTFAGVPWNAPYYARLGFCVIDAGELTPGLRRIRDHEAAR"
     gene            520..1386
                     /locus_tag="VGB85_03005"
     CDS             520..1386
                     /locus_tag="VGB85_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY0133014.1"
                     /translation="MRVATLALPLSLWMLACQPGAVVVPDDPTAASLAQVEHLRSFIP
                     DATHVILDLRPADAYAAGHVPGSVHLDVAGLRAEVDGVPEQLAPRAQIGALLAAAGVD
                     LGDEIVVLDADAGPPAARLVWTLQHFGHAPARVRLLDGGWVAWVASGAPVTGEPTPTT
                     PASTAALGSAQPALLADAGWILAHQRDPAVLLLDVRSDEEWAAGRIPGAQHIPWQQAR
                     GAGGKLLPAPALRELYAQAMAAPTVVTYCKSGMRASVTWFALRLLGHPDVRLYDGSWN
                     EWGARPDLPVER"
     gene            1510..2106
                     /locus_tag="VGB85_03010"
     CDS             1510..2106
                     /locus_tag="VGB85_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133015.1"
                     /translation="MTARGSTIARTATVLSRNPLGLLALCLVIGEAIAAMFLLHPAGL
                     AASERLLLCGFVVVYPLCVVAAIYRLISRHHTKLYAPADFRDERCFLEVLRLTDPGQA
                     LPLASTRAIAGEDFALLEPEPAANSGRRPEPGVYRHGRDEYYVTWRQQVYLLRRDGQS
                     GPPQEVRALPRGCEPVPRQDWDHELRALADVAEQFARR"
     gene            complement(2148..>2627)
                     /locus_tag="VGB85_03015"
     CDS             complement(2148..>2627)
                     /locus_tag="VGB85_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2293 domain-containing protein"
                     /protein_id="HEY0133016.1"
                     /translation="AANLAAARTALAAERSTEAYAKRREGDAARRERAQAEYVREFAG
                     ELLAFLRFAPRWRALAELLATKVTEHATPVGSGTVARTERIPVAQRAEAAVIAWLRHQ
                     TTAYDRMVIPRVKGKRREVRGELAQISRAILDLHRVDRPHAAAACPLCVALPTAAPA"
BASE COUNT          323 a         1028 c          920 g          356 t
ORIGIN      
        1 gcgcgcggcc tcgtggtcgc ggatcctccg caggcccggc gtcagctcgc cggcgtcgat
       61 cacacaaaag cccagccgcg cgtaatatgg agcgttccaa ggcaccccgg cgaaggtggt
      121 cagggtgatc gccggcgagc ccgcagcgcg cgcccagccc tcggcggcgc ccagcaactc
      181 ccggccgagg ccccgccgcg cgtgcccggg gtgcaccgac atctgctcga cgtgggcgca
      241 gccgtcgacg acctcgagca gcagatacgc gaccggccgc cccgcgtcgt cgtcgatcac
      301 ccacgcccgc ccgtcgcgct ggaagcccgc gagcaccgcg agcgcgggcg gctcgtcgtc
      361 ggcgatcgtc gccatgccga tcgcgcgaaa cggtgccccc gcggcccgtt cgagctcgcg
      421 gagcgccggc aagtcgtccg ccgaagcgag gcgcagcacc cggccatcgt gcccgatccg
      481 cctcgccgcg accagccgcc gcatgtgcta tccggagaca tgcgcgtcgc cacgctcgcc
      541 ctgcccctct cgctgtggat gctcgcctgc cagcctgggg ccgtcgtcgt gccggacgac
      601 cccacggccg cgagcctggc ccaggtcgag catctgcggt ccttcatccc cgacgcgacg
      661 cacgtgatcc tcgacctgcg cccggccgac gcgtacgccg cgggtcacgt gcccggctcg
      721 gtgcacctcg acgtcgccgg cctgcgcgcg gaggtcgacg gtgtgccgga gcagctggcc
      781 ccgcgggcgc agatcggggc gctgctcgcc gccgccggcg tcgacctcgg cgacgagatc
      841 gtcgtgctcg acgcggacgc cggcccaccc gcggcgcgcc tcgtgtggac cctccagcac
      901 ttcggccacg cccccgcgcg cgtgcgcctg ctcgacggcg gctgggtggc gtgggtcgcc
      961 agcggcgcgc cggtcaccgg cgagcccacg cccaccacac cggcctcgac cgccgccctc
     1021 gggtcggcgc agcccgccct gctcgccgac gccgggtgga tcctcgccca ccagcgcgac
     1081 cccgcggtgc tgctgctcga cgtgcgtagc gacgaggagt gggccgccgg ccggatcccc
     1141 ggcgcacagc acatcccgtg gcagcaagcc cgcggcgccg gcggcaagct gctgcccgcc
     1201 cccgcgctgc gcgagctgta cgcgcaggcg atggccgctc cgaccgtcgt cacctattgc
     1261 aagtccggca tgcgcgcgag cgtcacctgg ttcgcgctgc gcctgctcgg ccacccggac
     1321 gtgcgcctgt acgacggctc gtggaacgag tggggcgccc gcccggacct gccggtcgag
     1381 cgttgaagtc gagataccac gattcgcggc gacgcccccg tggaccgcga agctgtaatt
     1441 tccgtatgcc agagtaccgg atttacaaca ctcaccatac cgcgccacgt ggtatgagca
     1501 gttgcgagca tgaccgccag gggttcgacc atcgcccgaa cggcgaccgt gctgtcacgt
     1561 aatccgctcg ggttgctggc gctgtgcctg gtgatcggcg aggcgatcgc ggcgatgttc
     1621 ttgttgcacc ccgcgggcct ggccgcgagc gagcggctgc tgctgtgcgg cttcgtcgtc
     1681 gtctatccgc tgtgcgtcgt cgctgcgatc tatcgcctga tcagcaggca ccacaccaag
     1741 ctgtacgcgc cggccgactt ccgtgacgag cgctgcttcc tcgaggtcct gcgcctgacg
     1801 gacccgggcc aggcgctgcc gctggcgtcg acgcgggcga tcgccggcga ggacttcgcg
     1861 ctgctcgagc cggagcccgc cgccaacagc gggcgccgcc cggagcccgg cgtgtatcgc
     1921 cacggccgcg acgagtacta cgtcacctgg cggcaacagg tgtacctgct gcgccgcgac
     1981 ggtcagtccg gtccgccgca ggaggtccgg gcgctgccac gcggctgcga acccgtgccg
     2041 cgccaggact gggaccacga gctgcgcgcc ctcgccgacg tcgccgagca gttcgcccgc
     2101 cgctgattcg ctgatccgct gattcgctga tccgctgatt cgctggttca tgccggcgcg
     2161 gcggtcggca gcgcgacgca cagcgggcac gccgcggccg cgtgcgggcg gtcgacgcgg
     2221 tgcagatcga ggatcgcccg cgagatctgg gccagctcgc cacgcacctc gcggcgcttg
     2281 cccttcacgc gcgggatcac catgcgatcg taggcggtcg tctggtgccg cagccacgcg
     2341 atgaccgccg cctcggcgcg ctgggccacg gggatccgct cggtgcgcgc gacggtcccg
     2401 ctgccgaccg gcgtcgcgtg ctcggtcacc ttcgtcgcga gcagctcggc gagcgcccgc
     2461 cagcgcggcg cgaagcgcag gaaggccagc agctcgcccg cgaactcgcg cacatactcg
     2521 gcctgcgccc gctcgcggcg ggccgcgtcg ccctcgcggc gcttcgcgta ggcctccgtc
     2581 gaccgctccg ccgcgagcgc cgtacgtgct gcggcgaggt tggcggc
//
LOCUS       DASWNJ010000163         3340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_356008, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000163 DASWNJ010000000
VERSION     DASWNJ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356008"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(408..1850)
                     /locus_tag="VGB85_03020"
     CDS             complement(408..1850)
                     /locus_tag="VGB85_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133017.1"
                     /translation="MRRSLKPSPRPPGHATHLRSSKAKARGLTATISLGVCIGLHASI
                     AAAAPGTLSTAGTGVPRTEDGAGIKIGPRSLLHPGFALLIGGDSNVFWNRRQDVGGLR
                     PAAIVMPVAWIGIGNREVRDNTLQTPPEASERRMDYNLRVTAGYRAYLAGAEDIRKLP
                     RFSVDLNAFVVLFPGRRFSVGFTEFFSRIADPRNYDAGIGFNYNRVDHRGALGVTLRP
                     GGGRLSLSAAYTNEVLYYQSQDIYSGDRLVQGATTEVKWRLLPRSSLVLNYSFAHSYY
                     LSCNAEVDLDCKEDNNAHRVMGGFRGQIGARLTLDALAGYGAGIYYDDENGPNFKNFI
                     GVARLAYYPTLRTQLFAQLDRQFSDSLFGNYFVDAGGRIGGLHQWRWKMFTEVGFSVV
                     GRRYAGLPIPGSESRVVAYFNAPGFVRKDTILTLSGRVEQPFGRYFVLGARYDLVVDR
                     TDFAARYRGGLIDAGGFVKHVAFLVGAVRF"
     gene            complement(1928..2356)
                     /locus_tag="VGB85_03025"
     CDS             complement(1928..2356)
                     /locus_tag="VGB85_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133018.1"
                     /translation="MSVAQARGALERGMTELGELAARARQSGDALRIACVQDKQDRAG
                     AVMEVATSDMLVVQDPATDAQTRAFATDKLGESAEQMKGLVGQARACQGREEAADGKA
                     SNEARTPQGVLPQDPTGPGAPASRGLPPIDPRPPVASPVL"
     gene            complement(2626..>3340)
                     /locus_tag="VGB85_03030"
     CDS             complement(2626..>3340)
                     /locus_tag="VGB85_03030"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEY0133019.1"
                     /translation="EKLLAVEDDDKKKDKDPEIVDKVINPSKSYSKEAIAKARGVGVA
                     RVLGTYGGPGEGTVFDVIQSTENNLGELFAAGMTRVVDAEGNEVSGFVPGGEGISALG
                     AAVGTKGFDVSAAGPEVAGIDKKERKISLKSGAADIDGDADKKAVQATIRNRMGALQS
                     CYEKALRADAGLTGKIVYTITISVMGTVTNVQVGDDSLQDEGVKNCTVAKIKGWRFPT
                     TPGAEASSDVTFSVVFSGQ"
BASE COUNT          505 a         1109 c         1200 g          526 t
ORIGIN      
        1 agacgcccca ccgccagtcg agagcggcga gacagtggcg gtgaaagaga catgtccttc
       61 cggacaggtg gggcggcgag cacgtgctct gggaggaccc gcgtcgccgg gacccgcagc
      121 cggtcggccc ccgccccccg gcccaggaag cctcgccccg cgtcccccga ccacgccgtc
      181 gggcctgcga gcaccgtatc acccgagcac gccatcgggc acgccatcga gcctgcgagc
      241 accgcatcac ccgagcacgc catcgggcct gcgcgcaccg catcacccga gcacgcgccc
      301 gcgtcgcccg agtacacgcc cggagctccg agcgccgagc acgacgacca cgccgtcggc
      361 gtcacgcgag gatctctccc ttcaacttcc gacatacagg caggaagtca gaagcggacg
      421 gcgccgacga ggaaggcgac gtgtttgacg aagccgccgg cgtcgatgag gccgccgcgg
      481 tagcgggccg cgaagtcggt gcggtcgacg acgaggtcgt agcgggcgcc gaggacgaag
      541 tagcggccga atggttgttc gacgcggccg ctgagggtga ggatcgtgtc cttgcggacg
      601 aagcccgggg cgttgaagta ggcgacgacg cgcgactcgc tgccggggat cgggaggccg
      661 gcgtagcggc ggccgacgac cgagaagccg acctcggtga acatcttcca gcgccattga
      721 tgcaggccgc cgatccggcc gccggcgtcg acgaagtagt tgccaaacag cgagtcgctg
      781 aactgtcggt cgagctgcgc gaacagctgc gtgcgcaggg tcgggtagta ggcgagccgc
      841 gcgacgccga tgaaattctt gaaattcggg ccgttctcgt cgtcgtaata gatgccggcg
      901 ccgtagcccg ccagcgcatc gagggtcagg cgcgcaccga tctggccgcg aaagcctccc
      961 atgacccggt gggcgttgtt gtcctccttg cagtcgaggt cgacctccgc gttgcagctc
     1021 aggtagtagc tgtgcgcgaa gctgtagttg agcaccagcg aggagcgcgg cagcaggcgc
     1081 cacttgacct cggtggtcgc gccctggacc aggcggtcgc cgctgtagat gtcctgcgac
     1141 tggtagtaca ggacctcgtt ggtgtaggcc gccgagaggc tcaggcggcc gccgccgggg
     1201 cggagggtga caccgagggc gccgcggtgg tcgacgcggt tgtagttgaa gccgatgccg
     1261 gcgtcgtagt tgcgggggtc ggcgatgcgg gagaagaact cggtgaagcc gacgctgaag
     1321 cggcggcctg ggaacaggac gacgaaggcg ttgaggtcga cgctaaagcg cggcagcttg
     1381 cgaatgtcct cggcgccggc gaggtacgct cgatacccgg cggtgacgcg caggttgtag
     1441 tccatcctgc gttcgctggc ctcgggcggg gtctgcagcg tgttgtcgcg gacctcgcgg
     1501 ttgccgatcc cgatccacgc gaccggcatc acgatcgcgg cggggcgcag gccgccgacg
     1561 tcctggcggc ggttccagaa cacgttgctg tcgccgccga tcagcagggc gaagcccggg
     1621 tggagcagcg agcgcggccc gatcttgatc ccggcgccgt cctcggtgcg agggaccccg
     1681 gtgcccgcgg tcgacagcgt cccgggggcc gccgccgcga tcgatgcatg aagaccgatg
     1741 cagacaccca gcgagatcgt cgccgtcagg ccacgggctt tcgccttcga ggaccggagg
     1801 tgcgtcgcgt gcccgggggg ccgcggcgat ggctttaagg atcgacgcaa gggaggatct
     1861 aggcctacag cctaccgaaa ctccgccggc aagcgagtaa cccgcggtta aacgatgcgc
     1921 ggacgcctca cagcaccggg ctcgccactg gcgggcgcgg atcgatcggc ggcaggccgc
     1981 gcgaggccgg agcgccggga ccggtcgggt cctgcggcaa tactccctgc ggcgtgcggg
     2041 cttcgttgct cgccttgccg tctgcggcct cctcgcggcc ctgacaggca cgcgcctggc
     2101 cgacgaggcc cttcatctgc tcggccgact cgccgagctt gtcggtcgcg aaggcgcggg
     2161 tctgcgcgtc cgtggccggg tcctgcacga cgagcatgtc gctggtggcg acctccatca
     2221 cggcgccggc ccggtcttgc ttgtcctgca cgcaggcgat ccgcagggcg tcgccgctct
     2281 gccgggcgcg cgcggccaat tcgccgagct ctgtcatgcc gcgctcgagg gccccgcggg
     2341 cctgggcgac cgacatcgcg ggggccgggc tcgcgtcgct ggaggtcgca ctgtcctgag
     2401 cgacagcgtc ggaactggta gcgccggggc tcgcatcgcc gggcgtggtg ctcgcggcgg
     2461 gtgccgccaa aaccgctgtg ggagcgctct gtcgcggctt ctgcgtgacc tgtcctttcg
     2521 gactggtggc gacgggagct gcttggccgg atgcagtgga tgccgtcccc gacatcagca
     2581 agatgccgag ggtccatgcg acgaggggct tggtcggcgg catggtcatt gccccgaaaa
     2641 taccacagaa aaggtgacgt cagaggaagc ctcggcgccg ggggtggtgg ggaatcgcca
     2701 ccccttgatc ttggcgacgg tgcagttctt gaccccctcg tcctggagcg agtcgtcgcc
     2761 gacctgaaca ttggtaacgg tccccatgac ggagatggtg atcgtgtaga cgatcttgcc
     2821 ggtaagacct gcgtcggccc gcaaagcctt ttcataacag gactgaagcg cgcccatacg
     2881 attacggatc gtcgcttgca cggccttctt gtcggcgtcg ccgtcgatgt cggccgcgcc
     2941 gctcttgagc gagatcttgc gctccttctt gtcgatgcca gcgacctccg ggccggcggc
     3001 gctgacatcg aagcccttgg tgccgaccgc cgctccgagc gcgctgatgc cctcgccgcc
     3061 ggggacgaag ccgctgacct cgttgccctc ggcgtcgacg acccgggtca tgccggcggc
     3121 gaacagctca ccgaggttgt tctcggtgct ctgaattacg tcgaagaccg tgccctcacc
     3181 ggggccgccg taggtgccca ggacgcgggc gacgccgaca ccgcgggcct tggcgatcgc
     3241 ctccttcgag tacgacttgc tcgggttgat caccttgtcg acgatctccg ggtccttgtc
     3301 cttcttcttg tcgtcgtcct cgacggcgag cagcttctcg
//
LOCUS       DASWNJ010000164         2132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_462726, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000164 DASWNJ010000000
VERSION     DASWNJ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462726"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..>2132)
                     /locus_tag="VGB85_03035"
     CDS             complement(157..>2132)
                     /locus_tag="VGB85_03035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0133020.1"
                     /translation="ALGTLPQAVRAAIEKVALRRRIEAQRHELERSQNQLRVTLASIG
                     DAVIATDLAGDVTFMTAVAEGLTGWSSEAARGRPLAEVFVIVHEDTRQPGENPALRAL
                     REGRIVGLANHTILIARDGVERPIDDSAAPIRDGLGEVFGAVLVFRDVTERHRTEQSL
                     LLQSRVLDSMTEGVSVADEAGLIFYTNPAEDRMFGFARGELIGRRLTVGSAYADAEND
                     RRMREVHEQLAATGVWAGEWRNVRKDGTTFTTHARITTLVAAGRRCLVRVQEDVSDRH
                     RLEDELQQRVTELALAHRRKDEFLAMLAHELRNPLTPIKNALHILELRSRDVPTVERM
                     RELMTRQVRHFGRLVDDLLDVSRVTQGKLTLRPERLDLARLARDCGADHQAEFQRARV
                     ALTVDSPETPVWVTGDTTRLNQIVDNLLSNALKFTNPGGTVVLTVRCDGAQAELAVRD
                     DGIGIEPEVLTTVFDVFAQADKSLARSPGGLGLGLAIVKGLAQMHGGSVRAHSEGLGH
                     GTELTLSLPLQQEPPALTPASAPRTRPSRRQRILVVEDNRDSAESLRILLEVSGYEVR
                     VADTGTEGLRVAVEWQPDAVLCDIGLPEMDGFAVARALRSDPRTASATLIAVTGYGRE
                     EDVAAARAAGFDEHLMKPADPAKLLEKLAAGAS"
BASE COUNT          257 a          735 c          836 g          304 t
ORIGIN      
        1 agggcatcca cgcgggggcc ggcgaggcag cggcgacgat cgatgaggcc gagctgcgag
       61 agctcgcgct ggcgcaggtc gagcgcttgc cggcgcacac gctcgcctcg gcggtcggct
      121 tcctcggtta cctcgaggcg aagcgagcgt gaccgctcag ctcgcgccgg ccgcgagctt
      181 ctcgagcagc ttcgccgggt cggcgggctt catcaggtgc tcgtcgaagc cggccgcgcg
      241 ggcggccgcg acgtcctcct cgcgcccgta gccggtgacg gcgatcaggg tcgcgctggc
      301 ggtccgcggg tcgctgcgca gggcgcgggc gaccgcgaag ccgtccatct ccgggaggcc
      361 gatgtcgcac agcacggcgt cgggctgcca ctcgacggcg acgcgcagac cctcggtgcc
      421 ggtgtcggcg acccgcacct cgtaacccga gacctcgagc aagatccgca ggctctcggc
      481 cgagtcgcgg ttgtcctcga cgacgaggat ccgctggcgc cggctcggcc gcgtgcgggg
      541 cgcggacgcg ggggtcaggg ccggcggctc ctgctggagc gggagcgaga gggtcagctc
      601 ggtgccgtgg ccgagcccct cgctgtgggc gcgcaccgat ccgccgtgca tctgcgcgag
      661 gcccttcacg atcgcaaggc cgaggccgag gccgccgggg ctgcgggcga ggctcttgtc
      721 ggcctgcgcg aacacgtcga acacggtcgt cagcacctcg ggctcgatgc cgatgccgtc
      781 gtcgcgcacc gcgagctcgg cctgcgcgcc gtcgcagcgg accgtgagga cgacggtgcc
      841 gccggggttg gtgaacttca gggcgttgct gaggaggttg tcgacgatct ggttcagccg
      901 ggtggtgtcg ccggtgaccc agacgggggt ctcgggcgag tcgacggtga gggcgacgcg
      961 ggcgcgctgg aactcggcct ggtggtcggc cccgcagtcg cgggcgaggc gggccaggtc
     1021 gagccgctcg ggccgaagcg tcagcttgcc ctgcgtgacg cgcgagacgt cgagcaggtc
     1081 gtcgaccagg cgcccgaagt gacgcacctg ccgggtcatc agctcgcgca tgcgctcgac
     1141 cgtcggcacg tcgcggctcc gcagctcgag gatgtgcagg gcgttcttga tcggcgtcag
     1201 cgggttgcgc agctcgtggg ccagcatggc gaggaactcg tccttgcgcc ggtgggcgag
     1261 cgcgagctcg gtgacgcgct gctgcagctc gtcctcgagg cggtggcggt cgctgacatc
     1321 ctcctggacg cgcaccaggc agcggcggcc ggcggcgacg agcgtcgtga tgcgggcgtg
     1381 ggtggtgaag gtcgtgccgt ccttgcggac gtttcgccac tcgccggccc acacgcccgt
     1441 cgccgcgagt tgctcgtgga cctcgcgcat gcggcggtcg ttctcggcgt cggcgtacgc
     1501 gctgccgacc gtcagccggc gaccgatcag ctcgccgcgc gcaaagccga acatgcggtc
     1561 ctcggcgggg ttggtgtaga agatgagccc ggcctcgtcg gcgacgctga cgccctcggt
     1621 catgctgtcg aggacgcggg actggagcag gagcgactgc tcggtgcggt gccgctcggt
     1681 gacgtcgcgg aacacgagca ccgccccgaa cacctcgccg agcccgtcgc ggatcggcgc
     1741 ggcggagtcg tcgatcggcc gctcgacgcc gtcgcgggcg atcaggatcg tgtggttggc
     1801 gaggccgacg atccgtccct cccgcagcgc ccgcagggcc gggttctcgc ccggctggcg
     1861 cgtgtcctcg tggacgatca cgaacacctc ggcgagcggc cggccgcgcg cggcctccga
     1921 cgaccagccg gtgaggccct cggcgacggc ggtcatgaag gtcacgtcgc cggcgaggtc
     1981 ggtggcgatc acggcgtcgc cgatgctggc gagggtcacc cgcagctggt tctggctgcg
     2041 ctccagctcg tggcgctggg cctcgatgcg ccggcgcagc gcgaccttct cgatcgcggc
     2101 gcgcacggcc tgcggcaggg tccccagcgc cg
//
LOCUS       DASWNJ010000165         6486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_533220, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000165 DASWNJ010000000
VERSION     DASWNJ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6486
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533220"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VGB85_03040"
     CDS             complement(<1..257)
                     /locus_tag="VGB85_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133021.1"
                     /translation="MHPPIDPSTARFGPWRLRLTAWTGLGVVIEGLEHADGVAAPADY
                     VDEVLLTTGHREGFLALIDAAGLVVCKNVGGDDATHRDVRGR"
     gene            complement(305..769)
                     /locus_tag="VGB85_03045"
     CDS             complement(305..769)
                     /locus_tag="VGB85_03045"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0133022.1"
                     /translation="MHKITPFLWFNDNAEEAINFYVSVFKDSKVGTMTRRNGAVFSAT
                     FQLEGQQFMALNGGPHFKFTEAISLFVNCETQEEVDTLWDRLIADGGQPSQCGWLKDR
                     FGLSWQIIPSVLGRLMHDEDPARAGRVMQAMMQMQKIDIRKLKQAYEGETAA"
     gene            complement(807..1469)
                     /locus_tag="VGB85_03050"
     CDS             complement(807..1469)
                     /locus_tag="VGB85_03050"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0133023.1"
                     /translation="MDTSSSKRIRDAAGTRRQLVAAATRLMMRQGFAATTVDQICAEA
                     GLSKGSFFHNFASKEAVGLEAMAGFAKAGMDLYAAAAGEGPGADPLAELHRLLDIMAG
                     LVEQHGDELMCMVGMLAQELSATNATVREAAAEHMNAWVEMVARLLVKAQQRHRPQHD
                     FDATEVAWTLYSIWQGSMLIGKTLRSPATIAANLGHGRRYLDLLFTPRARVRTKRPAR
                     SE"
     gene            1560..1802
                     /locus_tag="VGB85_03055"
     CDS             1560..1802
                     /locus_tag="VGB85_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133024.1"
                     /translation="MKVILFGSTGMVGQGALRECLLDPGVTDVIAVTRAPTGLQHEKL
                     REVVHHDFTDFSNLQLDGDACFWCLGVSSAGMREAD"
     gene            1860..2201
                     /locus_tag="VGB85_03060"
     CDS             1860..2201
                     /locus_tag="VGB85_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133025.1"
                     /translation="MTFIFVSGTGAQSNAMWARVKRRTEDDLFAMPFKAVYVLRPGVI
                     LPLHGIRSRTRMYNLLFPVFYPLVMLVKLVAPRLITNTERVGQAMLRVARQGSVKKIL
                     ENPDINAAAQQ"
     gene            complement(2223..2537)
                     /locus_tag="VGB85_03065"
     CDS             complement(2223..2537)
                     /locus_tag="VGB85_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY0133026.1"
                     /translation="MQQAWQGPSRSTTSNRTMTPIAANGPYHCVLLGAGILDTCGGPE
                     VDAGARILRSGGDPVPRLFGAGNCIASPTGHAYWGAGGTIGPALVFGWIAGRNAAALS
                     PN"
     gene            3410..4999
                     /locus_tag="VGB85_03070"
     CDS             3410..4999
                     /locus_tag="VGB85_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133027.1"
                     /translation="MKLLPGALAELGNAATSIAIQRRTALLELEASLRSKPRKPSSAP
                     PVHAPGFLLWGISQDAISKSQDLIFDGHDFIEFLTAADCRLFEMFAPAGYGKTTLLVA
                     LARYLQSRAWFNFQGLFYWRCDGASPALHDELFEFVRRLGTAAVRPPASGSRVDWLLK
                     ALGDRPVVLIFDQLASQGGAVDDADARSLISELVARLGDGDGGSVKCVLATREKRPHP
                     RRGRHTTFPVPPLRPGEIRQLFQNCSVEFADDGRSERLHGWPRGLAAVAQYLRDICDG
                     RIDRINHLAVFQFADEVAPRDHERARRLRRGLMDALGFSPGQMKSQEYVFIDERLVVF
                     RLTLPDGRLLGELLPSDFVATAPNQMSLRDALLGLMTRQRVEWLFCHAKRVLEKLHEH
                     GELPRVDQSFRSAIVVATCSEDDGHWRVWRDIRQGLHHSLVAHWPLDSDPLATSDSGR
                     CLYLVCTCSEEPTVEEESQRDIHLRFADDTLLWAPMVWGAGIDELQGRKPWASDPLMA
                     LRAEDRLAQAIESLIRQNFAP"
     gene            5088..>6486
                     /locus_tag="VGB85_03075"
     CDS             5088..>6486
                     /locus_tag="VGB85_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133028.1"
                     /translation="MPIACTASLGGWTPLPPDFDLDLERALAARKNTQERQVFLAGFA
                     VQDALWSQPAGQMYAALKATTGEAVHLFVLTRRLNGPNGEWVGHALARLRAADPPPVP
                     GVVSALHVAIVGGQLCLTYRPFGTRTLSAACAAGSPRDILASFIATGEVLAAAHAAGL
                     VHGDFSPDYVMLEADGAPRVFGFGLAEALGLHNWGAPAKLLCNEYAAPESQIGPSKAS
                     TDQKTAADQYNFAASLKASLLARAALPPELARMLTRAMHPAPGLRYPQLAELVRDLRT
                     VRLPGERKGLLARFATRVPHGSSNMRRAAAGLAAGVVAAGLGSGGLQYYRIQVARTIL
                     CNEPAMAARERLASALSEAIPRPTWEAQWRRIPAVLDGWDRFWLCEPRTTLTAEEQPV
                     QACRAIQDRRMAHIAELVEERKDQSEDNVLLATYRAGISTYMVGDPETCSDVVHEPTE
                     GVDDEVLLDAYAKIEN"
BASE COUNT         1066 a         2133 c         2160 g         1127 t
ORIGIN      
        1 cgaccgcgga cgtcgcggtg ggtcgcatca tcgccgccga cgttcttgca cacgaccaga
       61 ccggccgcgt cgatcaacgc gaggaagccc tcgcggtgcc cggtcgtcag cagcacctcg
      121 tcgacgtagt cggctggcgc ggcgacgccg tccgcgtgct cgaggccctc gatcacgacc
      181 ccgagcccgg tccaggcggt caagcgcagg cgccagggcc cgaagcgcgc ggtagagggg
      241 tcgatcggcg ggtgcatcgc gggcgatgca tataccgcac ctcacgcccc ggcgcagcag
      301 cgcgtcaggc ggcggtttcg ccctcgtacg cctgcttgag cttgcggatg tcgatcttct
      361 gcatctgcat catcgcctgc atgacgcgcc ccgcgcgggc cgggtcctcg tcgtgcatca
      421 ggcggccgag caccgacggg atgatctgcc aggacaggcc gaatctgtcc ttgagccagc
      481 cgcactggct cggctggccg ccgtcggcga tcagccggtc ccacagcgta tcgacctcct
      541 cctgcgtctc acagttgacg aacagcgaga tggcctcggt gaacttgaag tgcggcccgc
      601 cgttgagcgc catgaactgc tggccttcga gctgaaaggt cgccgagaag acggcgccgt
      661 tgcgacgcgt catcgtcccg accttcgagt ccttgaacac ggacacgtaa aagttgatcg
      721 cctcctcggc gttgtcgttg aaccacaaaa atggggtgat cttgtgcatg cttgctcctt
      781 gtcgaacagt cttcgtcgtc gtgcgatcat tcgctgcgcg ccggtcgctt cgtgcgtacc
      841 cgggcgcggg gggtgaaaag taggtcgagg tagcggcggc cgtggccgag gttggcggcg
      901 atcgtcgccg ggctgcgcag ggtcttcccg atcagcatcg acccctgcca gatgctgtac
      961 agcgtccacg cgacctccgt cgcgtcgaag tcatgctgcg gacggtgccg ctgctgcgcc
     1021 ttgacgagca agcgcgcgac catctcgacc catgcattca tgtgctcggc cgccgcctcg
     1081 cggacggtcg cgttggtcgc ggacaattcc tgcgcgagca tgccgaccat gcacatcagc
     1141 tcgtcgccgt gctgctcgac caggcccgcc atgatgtcga gcaggcgatg cagctccgcc
     1201 agcggatcgg cgcccggccc ctcgccggca gcggccgcgt acaggtccat gcccgccttc
     1261 gcaaatcctg ccatcgcctc gaggccgacc gcctccttgc tggcgaagtt gtgaaaaaag
     1321 ctgcccttgc tcaacccggc ctcggcgcag atctggtcca ccgtcgtcgc agcgaagccc
     1381 tgccgcatca tcagccgcgt cgccgcggcg acgagctgac gccgcgtacc cgccgcatcc
     1441 ctgatccgct tgctgctgga cgtgtccata ccgaccagtt ggtctgtaac tcgggcgcgc
     1501 gatcctgtca accaggaaat tcgccccgcc cgtggcgacc gtgggacaag ggccgggcca
     1561 tgaaggtcat tcttttcggg agtacgggca tggtcggtca gggcgcgctg cgcgagtgcc
     1621 tgctcgaccc cggggtcacc gacgtgatcg cggtgacacg cgcgccgacc ggcctacagc
     1681 acgagaagct gcgcgaggtc gtgcatcacg acttcaccga tttttccaac ctgcagctcg
     1741 acggtgacgc ctgcttctgg tgcctcggcg tcagctcggc gggcatgcgc gaggccgact
     1801 agacgcgcat cacccacgat tacacgctgg ccgccgcgaa ggcgatcgtg cgcccgacga
     1861 tgacctttat cttcgtctcc ggcaccggcg cgcagagcaa cgccatgtgg gcccgcgtca
     1921 agcgcaggac cgaggacgac ctgttcgcca tgcccttcaa ggcggtgtat gtattgcgcc
     1981 cgggcgtgat cctgccgctg cacggcatcc ggtcgcgcac gcgcatgtac aacctgctct
     2041 tcccggtgtt ctatccgctg gtgatgctgg tcaagctcgt ggccccgcgg ctgatcacca
     2101 acaccgagcg cgtcggccag gcgatgctgc gcgtcgcccg acagggatct gtcaaaaaga
     2161 tcctcgagaa ccccgacatc aacgccgccg cgcagcagtg agccccgcgg cggtgcgccg
     2221 gttcagttcg gcgagagcgc cgcggcgttg cgtccggcga tccagccgaa caccagcgcc
     2281 gggccgatcg tgccgcctgc gccccagtac gcgtggccgg tcggcgaggc gatgcagttg
     2341 ccggcgccga acaggcgcgg caccgggtca ccaccggacc gcaggatccg ggcgccagcg
     2401 tcgacctccg gaccaccgca ggtgtcgagg atgccggcgc cgagcagcac gcagtggtaa
     2461 gggccgttcg cggcgatcgg cgtcatcgta cgattactcg tcgtgctgcg cgacggaccc
     2521 tgccaggcct gctggatcgg cgtctcgccg cgccggaagt cggctcggcg cctcgcacga
     2581 ggcggctcgg gtcatgcccc accccgtcgt ccgcatcgac ccagcaccgt gtcgccagcg
     2641 gcggtatcga cccagaccat gagtgcgtgc gaactgccga cgcgcaggta tcgaggggat
     2701 cgacggtctg ggcgatgtcc ccatgctcaa catcaggcca taaacgtcga tcttggtccc
     2761 ggcagtcagc agcatcgcgc gaccgttgcc acgtacacct ggacattgag cacatcgcag
     2821 gtgcaggtcg ggcgctgcga gcgcgtccgg aggtggtcac ttgaaaacga aacaacgttc
     2881 tggccccccg accgcgagga tcgatgtgcg cgaccacctg ccgagcaggc tgcttggagg
     2941 ccttccgctg caaatgccgc aagaacggca tcgtcgcggg cagcgacgag ggtacggggt
     3001 cgaccatcga tattcaggtg ccgcgcagta gtttatgggc ggttgcacgc attacggcag
     3061 atcctgtacg ctgggcgcgg ttgtggacga cgatgacctg atccgtgagg cctgcagtct
     3121 cctgagaggt ctcctcccgg gcctgtcccc tgctcgcatc gtcgatctgg cgtacgagac
     3181 gaccgcgggc gccgagcctg ggcccgccga cgtgagccgg tatgagcggt tcatcggaga
     3241 cctgtggaac accgcctggc gtgggtctca ggagctcgca gcggtgaatc gagtgtcatt
     3301 ctgcgagcga ttggttctta cgatcctcgt tcggatgaag tgcagtccag agttcctgga
     3361 gaccgccgtg cggtcggaca acctaccgcg tcccctccgg gactacgtct tgaagttgct
     3421 gcccggggcg ctcgcggagc tcgggaatgc agcaacttcc attgcgatcc agcgaaggac
     3481 cgccctcctg gagctcgagg ccagtcttcg ttccaaaccg aggaagccga gctcagcgcc
     3541 ccccgtccac gcacccggct tcttgctgtg ggggatcagc caggatgcga tcagcaagag
     3601 ccaggacctg atctttgacg gccatgattt catcgagttt ctgacagccg cggattgtcg
     3661 cctgttcgag atgttcgcgc cagcgggcta cgggaagacc accttgcttg tcgcgctggc
     3721 gaggtatctc cagtcacgcg cctggttcaa ttttcagggg ctgttttact ggcggtgtga
     3781 tggtgcgtcg cctgcattgc acgatgaact cttcgagttt gtgaggcgac tcggcacagc
     3841 ggcggtgcgg cctccggcgt ccgggtcacg agtagattgg ctcctcaaag cgctgggcga
     3901 tcggccggtg gtgctgatct tcgatcagct cgcgtcacaa ggcggagccg tcgacgatgc
     3961 cgacgcgagg tccttgatat cggaattggt ggctcggctc ggcgacggag acggggggtc
     4021 ggtcaagtgc gtcctcgcaa cgcgagagaa gcgtccgcac ccgaggcgcg gcaggcacac
     4081 cacatttccc gtcccgcccc tgaggccggg cgagatccgc cagctgttcc agaactgcag
     4141 cgtcgagttc gccgacgatg gccggagcga acgcctccac ggctggccgc gcgggctcgc
     4201 ggccgtcgcc cagtatctgc gggacatatg cgacgggcgg atcgatcgca tcaatcacct
     4261 cgcggtgttt caattcgcgg acgaggtcgc tcccagggat cacgagcgcg cgcggcggct
     4321 ccgcaggggg ctgatggacg cgctcggatt ttcccccggc cagatgaaga gccaggaata
     4381 cgtgttcatc gacgaacgcc tcgtggtctt ccgcttgacg ctgccagatg gtcgactgct
     4441 cggagagctg ctaccgtcgg acttcgtcgc cacggcgccg aatcagatgt cgctacgaga
     4501 cgccctcctc ggactgatga cgcggcaacg cgtcgagtgg ctgttttgcc acgccaagcg
     4561 cgtgctggag aagctccacg agcacggtga gttgccgcgg gtcgatcaga gctttcggtc
     4621 ggcgatcgtc gtcgcaactt gcagcgagga cgacgggcac tggcgggtct ggcgcgacat
     4681 tcggcagggg ttgcaccatt ccctggtcgc ccattggcca ctcgactccg atccgctcgc
     4741 tacgtcggac tccgggcgtt gtttgtacct ggtctgtact tgttcggaag agccgacggt
     4801 ggaggaggag agtcagcgcg acatccatct ccggttcgcg gacgacacgc tgctgtgggc
     4861 gccgatggtg tggggcgccg ggatcgacga actccagggc cgcaagccat gggcgtccga
     4921 cccgctcatg gcgctgcgcg ccgaggacag gctcgcgcag gcgatcgaga gccttatccg
     4981 gcagaatttc gccccatgag accgccggtg cgcgttttcc acgattttct cgaccggggt
     5041 gatcaatgag cgaagcgcgg gacactagag aggcagagag ccctcccatg cccattgcct
     5101 gcactgcgtc gctcggtggc tggaccccat taccgcccga tttcgatctg gacctcgagc
     5161 gggcgctggc ggcgcgaaaa aatacacagg agcggcaggt gtttctggcg ggattcgccg
     5221 tccaggacgc actgtggtcg caacctgccg ggcagatgta tgcggcactc aaggcgacga
     5281 ccggcgaggc ggtccacctg ttcgtcctca cgcgccggtt gaacggcccg aacggtgagt
     5341 gggtaggcca cgcgctcgct cgtttacgcg cggcggaccc ccctccggtc cccggagtcg
     5401 tatccgcgtt gcacgtcgca atcgtcggcg gtcaactctg tctgacgtac cgccccttcg
     5461 gcaccaggac gctatcagcg gcctgcgccg ccggctcacc acgggacatc ctcgcgtcgt
     5521 tcattgcgac aggcgaggtt ctcgccgccg cccacgcggc ggggctcgtt catggcgatt
     5581 tttcgccgga ctatgtgatg ctcgaggcgg acggcgcgcc gcgggtgttc ggtttcggcc
     5641 tggcggaggc gttggggcta cacaactggg gagcaccagc gaagctcttg tgcaacgaat
     5701 atgccgcgcc agagtcgcag atcggtccga gcaaggcgtc cacggatcag aagacggccg
     5761 cggatcagta taattttgct gccagtctca aggcgtctct cctcgctcgc gcggcgctac
     5821 caccagagct agcccgtatg ctcacgcgag cgatgcaccc ggcgccaggg ctgcgttatc
     5881 cgcagctcgc ggagttggtc cgggatctac gtaccgtgag actgcccggc gagcgcaagg
     5941 ggctcctggc gaggttcgca acgcgcgtcc cgcacgggtc gagcaacatg cgacgcgcgg
     6001 cggcgggcct cgcagccggc gtcgttgcag ccgggctggg gtctggcggc ctgcagtatt
     6061 atcgcataca agtcgccaga accatccttt gtaatgaacc ggcgatggct gcccgcgaac
     6121 gtctcgccag cgcattgagc gaagccatcc cgaggccgac atgggaggcg cagtggcggc
     6181 gcatcccggc ggtgctggac ggctgggacc ggttctggct gtgcgagccg aggacgacgc
     6241 tcacggccga ggagcagccg gtgcaagcgt gtcgagcgat acaagatcgt cgtatggcgc
     6301 atatcgcaga gctcgtcgag gagcgcaagg accagagcga ggacaacgtg ctgctggcga
     6361 cctaccgagc cggaatcagt acctatatgg tcggtgatcc cgagacctgc tccgacgtcg
     6421 tccacgagcc gacagaaggg gtcgacgacg aagtattgct ggatgcctac gccaagatcg
     6481 aaaact
//
LOCUS       DASWNJ010000166        13084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_144229, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000166 DASWNJ010000000
VERSION     DASWNJ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 13084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13084
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144229"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            803..2896
                     /locus_tag="VGB85_03080"
     CDS             803..2896
                     /locus_tag="VGB85_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY0133029.1"
                     /translation="MRDNDPNRRAPGDPPVDPAVATEPEPGIPADDDDNILADTFDED
                     GPTTQEPPGAAQDEFADMDLEELEDDGRPPEAPKLRTPAAGARSSAAAGVDVEAEVSN
                     FVSTQVMNLITSETSVVMAVPQSALPDADAPKVPSRRTSAAQLRHDLRTPFNQIIGYS
                     EMLIEDAEASDNEFVVNNIGQVLKDAQQLLALIDEILDPHAQRPSQERLNRDLLFPLS
                     HLLSHVERLLHTSQQRGQDEISGDIERILEAVRRLDEIVSSGAVNRALSTTRQPPEPT
                     VMVRDPTGGVPRRGVTPHTDTGKILVVDDNESNRNMLSRRLMRQGHTVKLAENGLQAL
                     EMLRAEDFDLVLLDVMMPEMDGYQVLEHLHADDKLRGLPVIMISALDQLDTVVKSIEL
                     GAEDYLPKPFNPVVLRARIGACLEKKRLRDRERAYIRKLRSEQERSEQLLLNILPRPI
                     AERLKEGQRTIADVFPDVTVLFADLVGFTRMSEQLPPAELVAMLNKIFSMFDQLAEKH
                     GLEKIKTIGDEYMAASGLPMPRPDHAEAMAEMALDMLAVIERFNAKRNRGVRIRIGMN
                     CGPVTAGIIGTKKFAYDLWGDTVNIASRMESHGIANAIQVTEATYKRLRHKYAFQRRG
                     IIHVKGKGALCTYFLVGRRPQKSGAMDGASGPMSAASGSYPVIPVSSSASTSSAPVDE
                     GRPAPAAEEEEIAID"
     gene            3194..8962
                     /locus_tag="VGB85_03085"
     CDS             3194..8962
                     /locus_tag="VGB85_03085"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEY0133030.1"
                     /translation="MASSTRRLVRPIVCISPLLLAALACTPVPSTPDPELSEPTGPLE
                     ILARTVPDVSTTLVFPPPHVGRHDRPALDIAGTDETDPNTGFKVQSPQVDDADRSFEV
                     EGQTVRIVFNRPVKLPRPMKKDKPVPAAAGTVTIEPTVAGKAMWTSNNALEFTASKPF
                     DPQQTYTITVKDVATADGVTLTEPWTGKFTAEPRISIAGKVITYMPVAGEPKVVEIFP
                     NSGSKIAAGDPIRVVFDQPISAKQAAEVLRVRADLIPDHEQKGVQEPEIAVEMSNAKT
                     GRLEGYTLNPRQIVEIRGAGSFAADTDIVVEVDELTYRYSIAGPLELTKVGCGYSYDR
                     TACEWTAPKLRTDGREVVIEYTNPIAAKDAALKAAISVWPPVANLNVYSGSNYESAGR
                     ISLSGAFDPSTEYTVKLPALKDHFGNTTPAAEIIIETTPLAASVSMPEGVLILDAAAS
                     HSFTVTTRNVGRGQVRAWAVPDDAEALKKARTQVEQHEVPGAPPDVILAFRPAAMRNE
                     FVQTQVDLLSQLKPGKNYILTVTLEETAFGASPVNYPSWMSAAKSPMTLVTPGDEQAL
                     AVHTHVTPEATLVHVARLATGEPVLGAAFAINGEAQVGRLTDRNGFAVLPVGTAKAGT
                     SLLSVSEGGATLQVALAHGHGDSRLFPHLAAEFAPVLGDRRALVITDRGIYRPGSKVF
                     IKASVRRKLGEQIVPIAGTPMRVRVLGPTDDLLSELALVSDDMGSVAGEYQIPAEARV
                     GRHRIEVAEASDPETPLDEQVIQVAEFEPPRFTVDVDASVAAGNTLRATVLGKYLFGA
                     AMDRAQVEWTLTREPADLPSGPFTDAGFHFTDDRYSWWDEESEASANWSRADSGTLGP
                     DGTLAVTQKLDLGDAVGPQEFTFEADVADTSYRHIAGRGSVIVHPSERYVGVKIDKPW
                     GDVGVAIPVKLGVIDREGKPVAGVDIKAKLNLVDWSYSRKPRRKGSGYDYEWQRTVKP
                     IGECTAVSAATAVNCDLTPTENGSYEVITEIGGKPGGVGSLWAWGHSGGERKAVPTRG
                     RTIDIIADKGRYVPGETAKLMIQNPFPAATAIFTLEQGGLLSHQVIRLDDAAHVFEVP
                     VQAGHAPYVHATVTLLPIGGGDERTDWKIGALRIPVALDDVRLTASVRSDRDTYEPRE
                     PVTVTIDVKDKGVAVADAEVALAVVDEGILRMTNFHATDPATALRPGQPLRFQVGDTR
                     DALAALLNHSQTAGDGAGDGSSTVSNARKNFVQTAFWKPNLRTDSQGRATATFDLPDN
                     LTRFRMMAVIIDKAGKGAAAEGDFTVRRPVLMVPVVPRFAAVGDSFEAAAMVHNNTDQ
                     PLGATVLMNGAEQAVQVPAQGNLRVGFPLTAQVPGELKLDFAVKDASDKVRDAVISTI
                     PVDVAGLTERPHLTGAFVGHQEIKMEVPQSVLVGRGDQDFVTVQVGQNLWPELGARMQ
                     FLLEYPHGCVEQTTSGLLPLLAAKDILPRIGFTGLSSEELDKRIESGLKRLASMRTAS
                     GGLGYWPGDGAPNVYGTAYAARAIVAAKQANVKMPDGLLKGVEEYLQDRLLSDGVEAE
                     VQAAIAQSLSELGSLPASSADALHDRRTDQGVFGKASLAIALSGLKGQEDRVKALLDE
                     VEAAFDADGKLVKDRSNSDFYYFGSPTRTRAQVAIALGRLRPGTPLHTKLVQQLAGET
                     ESYTTQSTAFSLMAVAEQLRGQPAEGAIFSATLGETTLSPFKDLGGGSQEFHIPVADL
                     RGKPQTLVLGSDSKAALAFIVRGSWKRELGDAKGLAATSATDGPDIYRVITDARGEQI
                     DLTAVKPGTVLRVALLADLPVGDLDSSEMNYLAVTDRLPAGFEPIQPDLWTVARAPEV
                     SDKHPFAEMLRYGGSDASFIELRDDRVQIYFDRVWGERVLATYLVRASTPGSFVLPPA
                     AAEFMYVGDSLGYSSAGTVTVK"
     gene            8990..11425
                     /gene="pbpC"
                     /locus_tag="VGB85_03090"
     CDS             8990..11425
                     /gene="pbpC"
                     /locus_tag="VGB85_03090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HEY0133031.1"
                     /translation="MSTRQSRLRRWTLRLSLGTVAACAVTCGTWSLVRARVGDPAAQL
                     DDRWHAGHRVLDRRGQLLRELPSDTGVRGQPLELEAMGPRLIQATLTSEDADFYEPGG
                     VDGRAIARATAQNLRHGRMVSGASTITQQLVKLLDNRGSPRIRGITDKLGEAARAQNL
                     EAVLGKPEILREYLNRLPYGHGMVGPEAAARAYFGVASKDLSWAQAAFLAVLPRAPSY
                     LDPYAHPERVLLRQRALLNELHADGHLNAREHERALAEPIDLRPLERPFLAPHLINTL
                     IAEGDLSSGTATVTTLDTALQQSVEGLTRTHLASIAERGATDAAVIVVDNASGEVLAY
                     VGSADFHDVGIAGQVDMARALRQPGSTLKPFVYALAFADGHSAAEMTADVETTFAEPG
                     GNYRPENFDHGHEGPLPLREALAGSLNIPAVRLAFEQGPRKLLELLHTIGFASLDRPA
                     DYYGLALALGSGEVELRELAAAYATLARGGRYAPLRVTVDDPQTLGAPAETVPAIDPA
                     VAALVTESLADPLARVRGLHGRGPFSMPYPAAIKTGTSSGYRDTWTVGYTRERTVAVW
                     LGNADGAPTAKLTGASGAGPLFADVMARAMRDVPQRAPLWDAELLARVEVCPLSGLPA
                     GPACDERATRNFLADHLPEGTCDLHVHVRRDASAPAGWRCDADGRETAVVFPPVFDAW
                     LAERAAVGATPDPFGRPWLPRARVPGCAGPAAPVPSVRLSAPAPGSVFSMGLRHDHQT
                     IELRAELAEGGEAAARALGELEFVVDGVVAARSAWPYQARIPATPGDHEILARPADPR
                     LAARLEPVQFSVR"
     gene            11451..11939
                     /locus_tag="VGB85_03095"
     CDS             11451..11939
                     /locus_tag="VGB85_03095"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEY0133032.1"
                     /translation="MAPVTPTEETALAQLRGLTRVMDGAITIPGTGIRIGLDPILGLL
                     LPVVGDWIGAATSGYIIVQGARLGASRATLMRMAGNLAVDLLVGSVPVLGDIFDMGFR
                     ANERNLALISAHIEAPERRRRSDLAVVVGVMTGLLVLAVATISLSAWLIYSLIQAVAG
                     SF"
     gene            11952..12533
                     /locus_tag="VGB85_03100"
     CDS             11952..12533
                     /locus_tag="VGB85_03100"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY0133033.1"
                     /translation="MATSATAVPRATVTDLLAIAEDCRRHELIDGEIVEKASPTWDHS
                     EAQSSLIEQLKGPFQRGTGGPGGWWIGCEIDVQLAVDQVYRPDVVGWRRERSPEPPRG
                     SPTTVRPDWVCEVLSPTNAASDTVRKLRNYHRSGVPHYWLADPATRTLIVYRWQPEGY
                     LLALSAAAPERVRAEPFAAIELSVAALFGDEDD"
     gene            complement(12583..>13084)
                     /gene="dcd"
                     /locus_tag="VGB85_03105"
     CDS             complement(12583..>13084)
                     /gene="dcd"
                     /locus_tag="VGB85_03105"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEY0133034.1"
                     /translation="EAGQVRHVAERPIVSYGTSSYGYDVRCAPEFKIFTNINSTIIDP
                     KHFDQNSFVDVVSEACIIPPNSFALARTVEYFRIPRNVLTICVGKSTYARCGIIVNVT
                     PLEPEWEGHVTLEFSNTTPLPARIYANEGVAQMLFFESDEPCETSYRDRGGKYQGQRG
                     VTLPHT"
BASE COUNT         2023 a         4736 c         4493 g         1832 t
ORIGIN      
        1 gttcgctcca gtcggtcaag cgcatccctg acgcgttgcg aacgcggcgc gcgccatgga
       61 cggtgcagtc gactactgcc agtgagctcg ctcgactctg cgctccttcg cgccgaagtc
      121 gcgcgagtat gcgtacttct ccgcgtcttc tcttcatcgc gcctcatcgc gccacagcat
      181 cgacagtacg gatgcttgta tgtgcgagca gcgacggcgc tgcgtccgtc gacacgcggt
      241 gcgctcgtgc tcgcgtcgcg cgtcgcatgg tcatgctcga cgtcccctgt cctccgggtc
      301 atgctgtcgc gatcgctggc gtcgcgatcg tggacatcgg cgagcgcgtc gcgacaccgg
      361 cgtctggttc ggcgctcgag gcaccgacga agcactcggc agacgcgccg cagacgggct
      421 caaggggccg tcgccgcggg tctggtgtgg gggatcgctc ccccgggaag atcgctaatt
      481 ttgccgaaat cacggccgcg ccgggccgcc cgcagacacc cgtgcggacg ctgtcggccg
      541 aaaccggcgc agacgcgagg aagggctggt gcggcggtgg cggtcgcatg tccgcggcca
      601 cgttcgggca gggcgatccc gccctgtggt gcacgggggg cggccaagtc gaggttgtcc
      661 tcgccgcgtc ggccggcgag catggggcaa gcgaaccggc gagaagccgt gaccgctctc
      721 acgggggctg cggtaccatg ccccggtacg atctccgtta ggctccaacg ttccggaaac
      781 cgcgacgcca gcacctcatc gcatgcgaga caacgatcca aatcgtcgcg cgccgggcga
      841 tccaccggtc gacccggccg tcgccacgga accggagcca ggcatccccg cggacgacga
      901 cgacaacatc ctcgccgaca ccttcgacga ggacggcccg acgacccagg agcctcctgg
      961 agccgcacag gatgaattcg cggacatgga cctcgaggag ctcgaggacg acggccggcc
     1021 tcccgaggcg ccgaagctgc gaacgcccgc cgcgggcgcg cgctcgtccg ccgcggccgg
     1081 tgtcgatgtc gaggcggagg tgagcaactt cgtatcgacg caggtgatga acctgatcac
     1141 cagcgagacc tcggtggtga tggcggtgcc gcagtcggcc ttgcccgacg cggacgcgcc
     1201 gaaggtgccg tcgcggcgga ccagcgcggc gcagctgcgg cacgacctgc gcacgccgtt
     1261 caatcagatc atcggctaca gcgagatgtt gatcgaggac gcggaggcct cggacaacga
     1321 gttcgtggtc aacaacatcg gtcaggtgct aaaggacgcg cagcagctgc tggcgttgat
     1381 cgacgagatc ctcgacccgc acgcgcagcg gccgagccag gagcggctca accgcgacct
     1441 gctgttcccg ctgagccacc tcctgagcca cgtcgagcgc ctgctgcaca cgtcccagca
     1501 gcgcggccag gacgagatct ccggcgacat cgagcgcatc ctcgaggcgg ttcgccggct
     1561 cgacgagatc gtctcgagcg gcgcggtcaa ccgggcgctg agcaccacgc gtcagccgcc
     1621 cgagccgacg gtgatggtgc gcgacccgac gggcggcgtg ccgcgccgcg gcgtcacccc
     1681 gcacaccgac accggcaaga tcctcgtcgt cgacgacaac gagtcgaacc gcaacatgct
     1741 ctcgcggcga ctgatgcggc aggggcacac cgtcaagctc gccgagaacg gcctgcaggc
     1801 gctggagatg ctgcgcgccg aggacttcga cctcgtgctg ctcgacgtca tgatgccgga
     1861 gatggacggc tatcaggtgc tcgagcacct gcacgccgac gacaagctga ggggtctccc
     1921 ggtgatcatg atctccgcgc tcgaccagct cgacacggtg gtcaagtcga tcgagctcgg
     1981 cgccgaggac tacctgccca agccgttcaa cccggtcgtg ctgcgcgccc gcatcggcgc
     2041 ctgcctcgag aagaagcgac tccgcgaccg cgagcgcgcg tacatccgca agctgcgctc
     2101 ggagcaggag cgcagcgagc agctgttgct caacattctt ccgcgcccga tcgctgagcg
     2161 gctcaaggag ggccagcgga cgatcgccga cgtgttcccg gacgtcacgg tgctgttcgc
     2221 cgacctcgtc ggcttcacgc gcatgtcgga gcagctgccg ccggccgagc tggtggcgat
     2281 gctcaacaag atcttctcga tgttcgacca gctggccgag aagcacggcc tcgagaagat
     2341 caagacgatc ggcgacgagt acatggcggc cagcggcctg ccgatgccgc gccccgacca
     2401 cgccgaggcg atggcggaga tggccctcga catgctcgcg gtgatcgagc gcttcaacgc
     2461 caagcgcaac cgcggcgtac ggatccgcat cggcatgaac tgcgggccgg tcaccgccgg
     2521 catcatcggc accaagaagt tcgcgtacga cctgtggggc gacaccgtca acatcgccag
     2581 tcgcatggag tcgcacggca tcgccaacgc gatccaggtg accgaggcga cctacaagcg
     2641 gctgcgccac aagtacgcgt ttcagcgccg cggcatcatc cacgtcaagg gcaagggcgc
     2701 cctgtgcacc tacttcctgg tcggccgccg gccgcagaag tccggcgcca tggacggcgc
     2761 ctccgggccg atgtcggccg cctcgggctc gtacccggtg atcccggtgt cgtcctcggc
     2821 ctcgacctcg tccgcgccag tcgacgaggg gcgccccgcg ccagccgccg aggaagaaga
     2881 gatcgcgatc gactagcgtg acggcgagcg aggctcgccg tccgccgggt ccgtcgtgtg
     2941 cgacaggcgt gtggtgacgc tgttcgtcgc ggcgaactgc tctgcttcgc cgatgtaata
     3001 atgcgtaaca tcacggagaa cggcgctgca cgggcccgca gcggcccggc ggcgactagc
     3061 gatgccacga atcgtgacat cgccggttgc atcgcgccgc accagcgctc gcgcgctgcc
     3121 atttacgcgc ggagctttgc gtaactcctc gtctttctcg ccggctgtca gtcttgcaca
     3181 attgccgccc gccatggctt caagcacccg ccgcctcgtt cgaccgatcg tctgcatctc
     3241 accgctgttg ctggcggccc tcgcctgtac gccggtcccc tcgacgcctg acccggagct
     3301 gagcgagccg accgggccgc tcgagatcct cgcgcgcacg gtgccggacg tcagcaccac
     3361 gctcgtgttc ccgccgccgc acgtcggccg tcacgaccga ccggcgctcg acatcgccgg
     3421 caccgacgag accgacccga acaccggctt caaggtccag tcgccgcagg tggacgacgc
     3481 cgaccgcagc ttcgaggtcg agggccagac cgtgcgtatc gtgttcaacc ggccggtcaa
     3541 gctcccgcgg ccgatgaaga aggacaagcc ggtgcccgcg gccgccggca cggtgacgat
     3601 cgaaccgact gtggccggca aggcgatgtg gacctcgaac aacgcgctcg agttcaccgc
     3661 gagcaagccc ttcgacccgc aacagaccta cacgatcacc gtcaaggacg tcgcgaccgc
     3721 cgacggcgtc acgctgaccg agccgtggac cggcaagttc accgccgagc cgcgcatctc
     3781 gatcgccggc aaggtgatca cgtacatgcc ggtcgcgggc gagccgaagg tcgtcgagat
     3841 cttccccaac tcgggctcca agatcgccgc gggcgacccg atccgcgtcg tcttcgacca
     3901 gccgatcagc gccaagcagg cggccgaggt gctgcgcgtg cgcgccgacc tgatccccga
     3961 ccacgagcag aagggcgtgc aggagccgga gatcgcggtc gagatgagca acgcgaagac
     4021 cggccgcctc gagggctaca ccttgaaccc gcggcagatc gtcgagatcc gcggcgccgg
     4081 ctcgttcgcc gccgacacgg acatcgtcgt cgaggtcgac gagctgacct accgctactc
     4141 gatcgccggc ccgctcgagc tcaccaaggt cggctgcggc tactcctacg accgcaccgc
     4201 gtgcgagtgg accgcgccga agctgcgcac cgacggccgc gaggtcgtca tcgagtacac
     4261 caacccgatc gccgccaagg acgccgcgct caaggccgcg atcagcgtgt ggccgccggt
     4321 cgccaacctc aacgtgtaca gcggcagcaa ctacgagagc gccggtcgca tctcgctctc
     4381 cggcgccttc gacccgtcga ccgagtacac ggtcaagctg cccgcgctca aggatcactt
     4441 cggcaacacg accccggccg cggagatcat catcgagacc acgccgctgg ccgccagcgt
     4501 gtcgatgccc gagggcgtgc tgatcctcga cgccgccgcc tcgcacagct tcaccgtcac
     4561 cacccgcaac gtcggccgcg gacaggtccg cgcgtgggcc gtgcccgacg acgccgaggc
     4621 ccttaaaaaa gcccgcaccc aggtcgagca gcacgaggtg cccggcgctc ccccggacgt
     4681 catcctcgcg ttccggcccg cggcgatgcg caacgagttc gtgcagaccc aggtcgacct
     4741 gctctcgcaa ctcaagcccg gcaagaatta catcctgacg gtgacgctcg aggagaccgc
     4801 cttcggcgcg agcccggtca actatccctc gtggatgagc gccgccaagt cgccgatgac
     4861 gctggtgacc ccgggcgacg agcaggcgct cgccgtgcac acgcacgtga cgcccgaggc
     4921 gacgctggtc cacgtcgcgc gcctggccac cggcgagccg gtgctcggcg ccgcgtttgc
     4981 gatcaacggc gaggcgcagg tcggccggct gacggaccgc aacggcttcg cggtgctgcc
     5041 ggtcggcacc gccaaggcgg gcacctcgct gctatcggtg tccgagggcg gcgcgaccct
     5101 gcaggtcgcg ctcgcgcacg gccacggcga ctcgcggctg ttcccccacc tcgcggccga
     5161 gttcgcgccg gtgctcggcg accgccgggc cctcgtgatc accgaccgcg ggatctaccg
     5221 cccgggctcg aaggtgttca tcaaggcgag cgtgcgccgc aagctcggcg agcagatcgt
     5281 gccgatcgcc ggcacaccga tgcgcgtgcg cgtgctcggg cccaccgatg acctgctcag
     5341 cgagctcgcg ctcgtcagcg acgacatggg ctcggtcgcc ggcgagtacc agatccccgc
     5401 ggaggcccgc gtcgggcgcc atcgcatcga ggtcgccgag gcctccgacc ccgagacgcc
     5461 gctcgacgag caggtcatcc aggtcgccga gttcgagccg ccgcgcttca ccgtcgacgt
     5521 cgacgccagc gtcgccgccg gcaacaccct gcgcgcgacc gtgctcggca agtacctgtt
     5581 cggcgccgcg atggaccgcg cgcaggtcga gtggaccctg acgcgcgagc cggccgacct
     5641 gccgtccggg ccgttcaccg acgccggctt tcacttcacc gacgaccgct acagctggtg
     5701 ggacgaggag agcgaggcgt cggccaactg gtcgcgcgcc gacagcggta cgctcggccc
     5761 cgacggcacg ctggcggtca cgcagaagct cgacctcggc gacgccgtcg gcccgcagga
     5821 gttcaccttc gaggccgacg tcgcggacac ctcgtatcgc cacatcgcgg gtcgcggcag
     5881 cgtgatcgtg cacccctcgg agcgctacgt cggcgtcaag atcgacaagc cgtggggtga
     5941 cgtcggcgtg gcgatccccg tcaagctcgg cgtcatcgac cgcgagggca agccggtcgc
     6001 tggcgtcgac atcaaggcga agctcaacct cgtcgactgg tcctactctc gcaagccgcg
     6061 gcgcaagggc agcggctacg actacgagtg gcagcgcacc gtcaagccga tcggcgagtg
     6121 cacggccgtc agcgccgcga cggccgtcaa ctgcgacctg accccgaccg agaacggtag
     6181 ttatgaggtg atcacggaga tcgggggtaa acctggcggc gtcggctcgc tgtgggcctg
     6241 ggggcacagc ggcggcgagc gtaaggcggt gccgacccgc ggccgcacga tcgacatcat
     6301 cgccgacaag ggccgctacg tgcccggcga gaccgccaag ctgatgatcc agaacccgtt
     6361 cccggcggcg acggcgatct tcacgctcga gcagggcggc ctgctcagtc accaggtgat
     6421 ccgcctcgac gacgcggcgc acgtgttcga ggtcccggtg caggccggcc acgcgcccta
     6481 cgtgcacgcg accgtcacgc tgctgccgat cggcggcggc gacgagcgca ccgactggaa
     6541 gatcggcgcc ctgcggatcc ccgtcgcgct cgacgacgtg cgcctgaccg ccagcgtccg
     6601 cagcgaccgc gacacctacg agccgcgcga gccggtgacc gtcaccatcg acgtcaagga
     6661 caagggcgtc gcggtcgccg acgccgaggt cgcgctcgcc gtcgtcgacg agggcatcct
     6721 gcgcatgacc aacttccacg cgaccgaccc ggccaccgcg ctgcggccgg gccagccgct
     6781 gcgcttccag gtcggcgaca cgcgcgacgc tctcgccgcg ctgctcaacc acagccagac
     6841 cgcgggcgac ggcgcgggcg atggttcgtc gacggtctcc aacgcgcgca agaacttcgt
     6901 gcagacggcc ttctggaagc cgaacctgcg caccgacagt cagggtcgcg cgaccgccac
     6961 cttcgacctg ccggacaacc tgacgcgctt tcgcatgatg gcggtgatca tcgacaaggc
     7021 gggcaagggc gccgcggccg agggcgactt cacggtgcgc cgtccggtgc tgatggtccc
     7081 ggtggtcccg cgcttcgccg cggtcggtga cagcttcgag gcggcggcga tggtgcacaa
     7141 caacaccgac cagccgctcg gcgcgacggt gctgatgaac ggcgcggagc aggccgtgca
     7201 ggtgccggcg cagggcaact tgcgggtcgg cttcccgctg acggcgcagg tcccgggcga
     7261 gctcaagctc gacttcgcgg tcaaggacgc gagcgacaag gtccgcgacg ccgtcatctc
     7321 gacgatcccc gtcgacgtcg ccgggctcac cgagcgcccg cacttgaccg gcgcgttcgt
     7381 cgggcaccag gagatcaaga tggaggtccc gcagtccgtg ctcgtcgggc gcggcgacca
     7441 ggacttcgtg accgtgcagg tcggccagaa cctgtggccc gagctcggcg cgcgcatgca
     7501 gttcctgctc gagtacccgc acggctgcgt cgagcagacc acctcgggcc tgctgccctt
     7561 gctggccgcc aaggacatcc tgccgcggat cggcttcacg ggcctgtcgt cggaggagct
     7621 cgacaagcgc atcgagtccg gcctcaagcg cctcgccagc atgcgcaccg ccagcggcgg
     7681 cctcggctac tggcccggcg acggcgcgcc caacgtgtac ggtactgcct acgccgcgcg
     7741 cgcgatcgtg gcggccaagc aggccaacgt gaagatgccc gacggcctgc tcaagggcgt
     7801 cgaggagtac ttgcaggatc gcctgctcag cgacggcgtc gaggccgagg tccaggcggc
     7861 gatcgcccag agcctgtcgg agctcggcag cctgccggcg agcagcgccg atgcgctgca
     7921 cgaccgccgc acggatcagg gcgtgttcgg caaggccagc ctggcgatcg cgctgagcgg
     7981 cctcaagggc caggaggacc gcgtcaaggc cctgctcgac gaggtcgagg ccgccttcga
     8041 cgccgacggc aagctcgtca aggatcgcag caacagcgac ttctattact tcggctcgcc
     8101 gacccgcaca cgcgcacagg tggcgatcgc cctcggacgt ctgcgtccag gtaccccgct
     8161 gcacaccaag ctcgtgcagc agctcgcggg cgagaccgag tcgtacacca cgcagtcgac
     8221 ggcgttctcg ctgatggcgg tcgccgagca gctgcgcggt caaccggccg agggcgcgat
     8281 cttctcggca acgctcggcg agacgacgct gtcgccgttc aaggacctcg gcggcggcag
     8341 tcaagagttc cacatcccgg tcgcggacct gcgcggcaag ccgcagacgc tggtgctcgg
     8401 cagcgacagc aaggcggcgc tggcgttcat cgtgcgcggc agctggaagc gcgagctcgg
     8461 cgacgcgaag ggcctcgcgg cgaccagcgc gaccgacggc ccggacatct accgcgtgat
     8521 caccgacgcg cgcggcgagc agatcgacct cacggcggtc aagccgggca cggtgctgcg
     8581 cgtcgccttg ctggcggacc ttccggtcgg cgacctcgac tcgagcgaga tgaactacct
     8641 cgcggtgacc gaccgcctgc cggccggctt cgagccgatc cagcccgacc tgtggacggt
     8701 ggcccgcgcg cccgaggtct ccgacaagca ccccttcgcc gagatgctgc gctacggggg
     8761 cagcgatgcc agcttcatcg agctgcgcga cgatcgcgtg cagatctact tcgaccgcgt
     8821 gtggggcgag cgcgtgctcg cgacctacct cgtgcgcgcc tcgacccccg gcagcttcgt
     8881 gctgccgccg gccgcggcgg agttcatgta cgtcggcgac agcctcggat acagctccgc
     8941 cggcaccgtc accgtgaagt agacggggac cctgacgcgt tcgatcggca tgagcacccg
     9001 acaaagtcgc ctccggcgct ggaccctgcg cctgtccctc gggacagtcg cggcctgcgc
     9061 cgtcacctgc gggacctggt cgctggtgcg cgcgcgcgtc ggcgaccccg ccgcgcagct
     9121 ggacgaccgc tggcacgccg gccaccgcgt gctcgaccgc cggggtcaat tgctgcgcga
     9181 actgccgagc gacaccggtg tgcgggggca gccgctcgag ctagaggcga tgggcccgcg
     9241 gctgatccag gcgaccctga ccagcgagga cgccgacttt tatgaacccg gcggcgtcga
     9301 cgggcgggcg atcgcccgcg cgaccgcgca gaacctccgg catggccgca tggtctccgg
     9361 cgcgagcacg atcacccagc aactggtcaa gctgctcgac aaccgcggca gcccgcgaat
     9421 tagagggatc accgacaagc tcggcgaggc cgcgcgcgcg cagaacctcg aggccgtgct
     9481 cggcaagccc gagatcctgc gcgagtacct caaccgtctg ccctacggcc acggcatggt
     9541 cggccccgag gcggccgcgc gcgcgtactt cggggtcgcc agcaaggacc tcagctgggc
     9601 ccaggcggcc ttcctcgccg tgctgccgcg cgctccctcg tacctcgatc cctacgcgca
     9661 cccggagcgc gtgctgctgc gtcaacgggc cttgctgaat gagctccacg ccgacggcca
     9721 cctcaacgca cgcgagcacg agcgcgcgct cgccgagccg atcgacctgc gcccgctcga
     9781 gcgccccttc ctcgccccac acttgataaa caccctgatc gccgaggggg acctgtcctc
     9841 cgggacagcc accgtcacga ccctcgacac ggcgctgcag cagtccgtcg agggtctgac
     9901 gcgcacgcac ctcgccagca tcgccgagcg cggggcgacc gacgccgcgg tgatcgtcgt
     9961 cgacaacgcc agcggcgagg tgctcgccta cgtcggcagc gcggactttc atgatgtcgg
    10021 catcgccggc caggtcgaca tggcgcgggc gttgcggcag ccgggctcga cgctcaagcc
    10081 cttcgtgtac gcgctcgcgt tcgccgacgg tcacagcgcg gcggagatga cggccgacgt
    10141 agagaccacc ttcgccgagc ccggcggcaa ttatcgaccc gagaatttcg accatggcca
    10201 cgagggcccg ctgccgttac gggaggcgct ggccgggtcg ctcaacatcc cggcggtgcg
    10261 cctcgcgttc gagcagggcc cgcgcaagct gctggagctc ctgcacacga tcggcttcgc
    10321 cagcctcgat cgccccgcgg attattatgg cctcgcgctc gcgctcggca gcggcgaggt
    10381 cgagctgcgt gagctggccg ccgcgtacgc gacgctggcc cgcgggggca ggtatgcgcc
    10441 gctgcgcgtc accgtggacg acccgcagac gctcggtgcg ccggccgaga ccgtccccgc
    10501 gatcgacccg gccgtcgccg cgctggtgac cgagagcctc gccgacccgc tcgcgcgcgt
    10561 gcgcggcttg catggtcgcg ggccgttctc gatgccgtac ccggccgcga tcaagaccgg
    10621 cacgtcctcc ggctatcgtg acacctggac ggtcggctac acgcgcgagc gcaccgtcgc
    10681 ggtgtggctc ggcaacgccg atggggcccc gacggccaag ctgaccggcg cgtcgggggc
    10741 cggcccgctg ttcgccgacg tcatggcccg cgcgatgcga gacgtgccgc agcgcgcccc
    10801 cctgtgggac gccgagctgc tggcgcgcgt cgaggtgtgc ccgctctccg ggctgcccgc
    10861 cggccccgcg tgcgacgagc gagcgacccg caacttcctc gccgaccacc tgcccgaagg
    10921 gacatgtgac ctgcacgtcc acgtgcggcg cgatgcgagc gcgcccgcgg gctggcgctg
    10981 tgatgcggat ggtcgcgaga ccgccgtcgt gttcccgccg gtgttcgacg cctggctggc
    11041 cgagcgcgcc gcggtcggtg cgacgccgga ccccttcggt cggccgtggc tgccgcgcgc
    11101 gcgcgtaccc gggtgcgccg gtccggccgc accggtgccc tcggtgcggc tgtcggcgcc
    11161 ggcgcccggg agcgtgtttt cgatgggcct gcgccacgac caccagacga tcgagctgcg
    11221 cgccgagctg gccgagggtg gcgaggccgc tgcccgcgcg ctcggggagc tcgagttcgt
    11281 cgtcgacggc gtggtcgcgg cccgcagcgc gtggccgtat caggcccgca tcccggcgac
    11341 gcccggcgat cacgagatcc tggcccgtcc ggcggatccg cgactcgcgg cccggctcga
    11401 acccgtgcag ttcagcgtgc gctgagccgg gcccgcgcta tgttcgcggg atggcgcccg
    11461 tgaccccgac cgaggagacc gcgctcgcgc agctgcgcgg gctgacccgc gtgatggacg
    11521 gcgccatcac gatccccggc accgggatcc gcatcggcct cgacccgatc ctcggcctgc
    11581 tcctgccggt cgtcggcgac tggatcggcg cggcgacctc cggatacatc atcgtgcagg
    11641 gcgcccgcct cggggcctcg cgcgcgacgc tgatgcgcat ggcgggcaac ctcgccgtcg
    11701 acctgctcgt cggctcggtg cctgtcctcg gggacatctt tgatatggga tttcgcgcca
    11761 acgagcgcaa cctcgccctg atctcggcgc acatcgaggc cccggagcga cgccgccgca
    11821 gcgacctcgc ggtcgtcgtc ggcgtcatga ctggcctgct cgtgctcgcg gtggcgacga
    11881 tctccctcag cgcctggctg atctacagcc tgatccaggc cgtcgccggg tcgttctgat
    11941 acattgatcg catggcgacc tccgcgaccg cggtccctcg cgcgaccgtc accgacctgc
    12001 tggcgatcgc ggaggactgt cgccggcatg agttgatcga cggcgaaatt gtagagaagg
    12061 cgagtccgac ctgggaccac agcgaggcgc agtcctcgtt gatcgagcag ctcaagggtc
    12121 cgttccagcg cggcaccggc gggcccggcg gctggtggat cggctgcgag atcgacgtgc
    12181 agctcgcggt cgatcaggtg taccggcccg atgtcgtcgg gtggcggcgc gagcggagcc
    12241 cggagccgcc gcgcggctcc ccgaccacgg tgcggcccga ctgggtctgc gaggtgctgt
    12301 cgccgaccaa tgccgcgagc gacacggtcc gcaagctccg caactatcat cgcagcggcg
    12361 tcccccacta ctggctcgcc gaccccgcga cgcgcacgct gatcgtgtat cgctggcagc
    12421 ccgagggcta cctgctggcg ctctcggccg cggcgccgga gcgggtccgg gccgagccct
    12481 tcgcggcgat cgagctctcc gtcgccgcgc tgttcggcga cgaggacgac tgagccgcgc
    12541 tcaggtgtgc ggcctcaggt gtgcggcctc aggtatgcgg cctcaggtgt gcggcagggt
    12601 cacgccgcgc tggccctggt acttgccgcc gcggtcgcgg tagctggtct cgcagggctc
    12661 gtcggactcg aagaacagca tctgcgcgac gccctcgttg gcatagatgc gggcgggcag
    12721 gggggtcgtg ttcgagaact cgagggtgac gtggccctcc cactcgggct cgagcggggt
    12781 gacgttgacg atgatgccgc agcgcgcgta ggtcgacttg ccgacgcaga tcgtgagcac
    12841 gttgcgtggg atgcgaaagt actcgacggt gcgggccagc gcgaacgagt tcggcgggat
    12901 gatgcacgcc tcggacacga cgtcgacgaa gctgttctgg tcgaagtgct tcgggtcgat
    12961 gatcgtcgag ttgatatttg tgaagatctt gaactcgggc gcgcagcgga catcgtagcc
    13021 gtagctcgag gtcccgtaag acacgatagg gcgctcggcg acgtggcgga cctggcccgc
    13081 ctcg
//
LOCUS       DASWNJ010000167         1514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_144257, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000167 DASWNJ010000000
VERSION     DASWNJ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144257"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VGB85_03110"
     CDS             complement(<1..434)
                     /locus_tag="VGB85_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133035.1"
                     /translation="MRSLTRLVPALALFAALPACDGDDKDMMQESSTGEAESGSSGSE
                     SGSSEGGSETEAAEAVCDAETHGSSRPCEGGTQFCFTNDAVAYEWGSCVASPACVPGV
                     IDECESCVLDDHGVPTITDTCEDGTSTPLVLNFDGAPVEYGSA"
     gene            617..979
                     /locus_tag="VGB85_03115"
     CDS             617..979
                     /locus_tag="VGB85_03115"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY0133036.1"
                     /translation="MTESKKHTGGCHCGQVRFEVEADLAAPVIECNCSMCGKSGTRLS
                     FVPAARFNLLQGEDHLTDYLFAKQHIHHLFCDVCGIKPFARGAAPDGSEMIAINTRCI
                     DDIDLAAQQIQHFDGKSL"
     gene            complement(984..1373)
                     /locus_tag="VGB85_03120"
     CDS             complement(984..1373)
                     /locus_tag="VGB85_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133037.1"
                     /translation="MQSYARAKYRLSEDTEQRFALVFAIPGGRTQQIVVTHFSARERD
                     FIEFRTLVCPEAQLDPRAALLHNATVGEHNHSILGSLALYDGVYYLVYRALLATLDPE
                     EFELPLHAMALHADTLEARHTQGLDAY"
BASE COUNT          255 a          528 c          495 g          236 t
ORIGIN      
        1 gcgctgccgt actcgaccgg cgcgccgtcg aagttgagca ccagcggcgt gctcgtgccg
       61 tcctcacagg tatcggtgat cgtcggcacg ccgtggtcgt cgagcacgca cgactcgcac
      121 tcgtcgatca cgcccggcac acaggccggc gacgcgacac agctgcccca ctcgtacgcg
      181 acggcgtcgt tggtgaagca gaactgcgtg ccgccctcgc aggggcggct gctgccgtgc
      241 gtctccgcgt cacacaccgc ctcggcggcc tcggtctcgg agccaccctc ggacgacccg
      301 gactcggagc ccgaggagcc cgactccgcc tcacccgtgc tcgactcctg catcatgtcc
      361 ttgtcgtcgc catcgcaggc gggcagggcg gcgaacaggg cgagggcggg gacgaggcgg
      421 gtcaggctgc gcatgctgaa gaccccctta gcgaagggcg tgccaccttg agatcacaca
      481 gttatttcga aaccggctcc gcaggagcgc cgcaccggta gggcaagcct gtcgcggtgc
      541 gacggcgcgg cgcccggggg ctgcaggacg ctgtttttcc gccacaagat cgcgcctcgc
      601 tggcaccctc gcgcgcatga ccgaatccaa gaagcacacc gggggctgtc actgcggaca
      661 ggtacgtttc gaggtcgagg ccgacctcgc cgcgccggtg atcgagtgca actgttccat
      721 gtgcggcaag agcggcacgc gcctgagctt cgtgcccgcc gcgcggttca acctgctgca
      781 gggcgaggac cacctcaccg actacctgtt cgccaagcag cacatccatc acctgttctg
      841 cgacgtctgc gggatcaagc ccttcgcccg cggcgccgcg ccggacggca gcgagatgat
      901 cgcgatcaac acccgctgca tcgacgatat cgacctcgcc gcgcagcaga tccagcactt
      961 cgacggcaag agcctgtgag ccgtcagtac gcgtcgaggc cctgcgtgtg ccgggcctcg
     1021 agcgtgtcgg cgtgcagcgc catcgcgtgc agcggcagct cgaactcctc ggggtcgagc
     1081 gtcgccagca gggcgcgata tacgaggtaa tataccccgt cgtacagcgc cagcgacccg
     1141 aggatcgagt ggttgtgctc gccgacggtc gcgttgtgca gcagcgccgc gcgcgggtcg
     1201 agctgcgcct ccggacatac cagcgtgcga aactcgatga agtcgcgctc cctcgccgaa
     1261 aagtgcgtca cgacgatctg ctgcgtgcgc ccgccgggga tcgcaaacac cagcgcgaac
     1321 cgctgctccg tatcctccga caaccggtac ttcgcgcgcg cgtagctctg caacttgtat
     1381 gttcggcggg cgttgagcga gagttcctgt ccaaagactc cgggagaccg atctgaacgt
     1441 gggtctcgag cccgaggctg agcaaggtcc ccggagtcga atctgtcctc ttgagcccgg
     1501 acaactgcac gagc
//
LOCUS       DASWNJ010000168         1783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_144275, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000168 DASWNJ010000000
VERSION     DASWNJ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144275"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VGB85_03125"
     CDS             <1..192
                     /locus_tag="VGB85_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEY0133038.1"
                     /translation="HAGHPSETVGPAKQRKIVRAATAWLVRASLWEQVAVRFDVVAIV
                     FGTEDKAPEITWITGAFDG"
     gene            complement(236..811)
                     /locus_tag="VGB85_03130"
     CDS             complement(236..811)
                     /locus_tag="VGB85_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133039.1"
                     /translation="MLLVPLLSACPKGDVGAPCNHGDVQAPDSQVVTFPALSCNDLLC
                     VFAQKDTPPKAPCAANPDCNTDGGTRFACVNKACKLSSTYVLERSMCSKTCSTDADCA
                     DGGIGKKVQAQDNSCENGFQCAPIQKLGEFCCQKLCVCNDDVSAGTVAKLNEDCAAFD
                     KDAEGNPMCNVMEMTPGMTTDAATTMDATMP"
BASE COUNT          280 a          639 c          566 g          298 t
ORIGIN      
        1 cacgccggtc accccagcga gacggtcggc ccggccaagc agcggaagat cgtccgcgcc
       61 gcgaccgcct ggctggtccg cgcctcgctg tgggagcagg tcgccgtccg cttcgacgtc
      121 gtggcgatcg tgttcggcac cgaagacaaa gccccggaga tcacatggat caccggggcc
      181 ttcgatggct gaccatcgcg gtcaggccgt cagggtcatc agggccatca caccatcagg
      241 gcatggtcgc gtccatcgtc gtcgccgcgt cggtcgtcat gccgggcgtc atctccatga
      301 cgttgcacat cgggttgccc tcggcgtcct tgtcgaacgc ggcgcagtcc tcgttgagct
      361 tggcgacggt gccggcggag acgtcgtcgt tgcacacgca cagcttctgg cagcagaact
      421 cgccgagctt ctggatcggc gcgcactgga agccgttctc acaggagttg tcctgcgcct
      481 ggaccttctt gccgatgccg ccgtccgcac agtcggcgtc ggtcgagcag gtcttcgagc
      541 acatcgagcg ctcgagcacg taggtcgacg agagcttgca ggccttgttg acgcaggcga
      601 agcgggtgcc gccgtccgtg ttgcagtccg ggttcgcggc gcacggagcc ttgggcggcg
      661 tgtccttctg cgcgaacacg cagagcaggt cgttgcagct cagggccggg aaggtcacca
      721 cctggctgtc gggcgcctgc acgtcgccgt ggttgcaggg cgccccgacg tcacccttgg
      781 gacaggcgct cagcagcgga acgaggagca tggcacccag caacgtggcg gcggtgcgga
      841 gggaggcgag cgtgcgaatg gctggcatgt gggatctcgg tctagacggc cggaaggggc
      901 cttcatcgta cacgtacagg ccatagggtc aagcgttccg accccgcgcc aaaactcgcg
      961 cattcgcttc tcaactggcg caacaacctc gccccgtggg agcacgcgac cgcgacgtca
     1021 tccgcgaggt ccgcggcgat catgccgccg tggtcgactg ccggagatcg ttccgtcgca
     1081 agtcgtcgcg agttcgcctc gccgtcgttg gcgccggctg tcgtcgcaag gccccacgcg
     1141 ggcccccgcg tcgcagacat cgagccgtaa tattagtcac aggtcgttga aagcctcgcc
     1201 ccggaattct acaactgcgc cgagatctcc acagatccag gcgcagacca gtccgttcca
     1261 tgtgcttcga gccgtctgcc ctcggctccc gtactcgtcc atgaccccgg cgaccatgac
     1321 gcttcaggtc cccctgtgca ccctcgcccc gaatcaccac catggagctc ggccgcgcga
     1381 aaatctccat cgccacgctt gcgaaactcg gcgcagcacg agccccttgc gactcgaagc
     1441 tggccgctgg taatgtccaa gcctctatga gtgcgaactg cacgtccggc gcccgcgccg
     1501 gagtactttc gcggctcaag acatcgaggc aaaggcttgg cttcccgccc ggcagtaccc
     1561 cgctcgctga ggacgcaacg acagcatgaa atcgctccga cacctcacgt cactcactct
     1621 tctcagtctt tgcgccacgt gggccctcta ctccccaccc gccgaggcag gtccgccgcc
     1681 gccgccgccc gcaggcaaga cgccgcctcc gccctcgggt ggtccggccg tgcctccgcc
     1741 gtcgaccgga gctgccgtgc cgccgccgtc cgccggcgcc gcg
//
LOCUS       DASWNJ010000169         1689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_462845, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000169 DASWNJ010000000
VERSION     DASWNJ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462845"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..1672
                     /locus_tag="VGB85_03135"
     CDS             17..1672
                     /locus_tag="VGB85_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133040.1"
                     /translation="MARATEALAGLGVAALLFAGARALLRDSAQGEPFRVEVEVPARV
                     VASSEGLRWQGTVRPATLAERVEVWVDGVRLEASNIEGQIAADLHAVGPGHHLVELAV
                     TQQGGRTQTVTDTLLVGPFAAVQEDCALSLTVAEAAIQRLLIPPLRERLLAAAREDPL
                     LGPGTTLERAELTLLVDAVRVRVTLAGINTIAVDAFLKLQPAGQRGLALSLGWLGPVE
                     FFGKLRTQATLGGAAVGAALTGPLAPLGAVAGYVLADRYVGKRAHAEVREQLRRGLEQ
                     VASVPLLPSSAELLPGHPRSAVTLAFCGPVTIAPGVGISTRLSLRSPAPGGAVYRGVV
                     LPEPDPSDMSDVRLDLSIDAVNALLHAWTANGLLADRMAAAQWVGQVDRALQEWTLLS
                     LVGVTVTRAPVLMAADQGNAAPRRDAWALSFGGLVLDLRGGEHAGTVVAAGRGWITPE
                     LDAGSGRLELGGVVDRLRLTCIEGQVLTPCFGALLELGEVESRLDASLAPGTGRLPGI
                     DVRGLLRTRTRALRPEGLDVEELTVTVPAGHPGVLRVRARLRP"
BASE COUNT          190 a          548 c          695 g          256 t
ORIGIN      
        1 tccctgatcg tcgtgtatgg cgcgcgcgac tgaagctctg gccgggctcg gcgtcgccgc
       61 gctgctgttc gcgggggcgc gtgcgctgct gcgagacagc gcgcagggcg agccgtttcg
      121 cgtcgaggtc gaggtgccgg cgcgggtggt ggcctcgagc gaggggctgc gctggcaggg
      181 cacggtgcgc ccggcgacgc tcgcggagcg cgtggaagtg tgggtcgatg gggtgaggct
      241 cgaggcgagc aacatcgagg ggcagatcgc cgccgacctg cacgccgtcg ggccggggca
      301 tcacctcgtc gagctcgcgg tgacgcagca gggcggccgc acacagacgg tcacggacac
      361 gttgctcgtc gggccgttcg cagcagtgca agaggattgc gcgctgtcgc tgacggtcgc
      421 ggaggcggcg atccagcggc tgttgatccc gccgctgcgc gagcgactgt tggcggcagc
      481 ccgcgaggat ccgctgctcg ggcccgggac cacgctggag cgcgccgagc tgacgttgct
      541 cgtcgacgcg gttcgcgtgc gcgtgacgct ggccggcatc aacacgatcg cggtcgatgc
      601 atttttaaaa ttgcagccgg cggggcagcg cgggctggcg ctgtcgctgg ggtggctcgg
      661 gccggtcgag ttttttggaa aactgcgcac gcaggcgacg ctcggcgggg cggcggtcgg
      721 ggcggcgctg accggcccgc tggcgccgct gggggcggtg gcgggatatg tcctcgcgga
      781 caggtacgtc ggcaagcggg cgcacgcgga ggtgcgcgag cagctgcgcc gtggactcga
      841 gcaggtcgct agcgtgccgc tgttaccgtc cagcgccgag ctgttaccgg gccacccgcg
      901 ctcggcggtc acgctggcgt tctgtggacc cgtgacgatc gccccgggcg tggggatctc
      961 gacgcggctg tcgctgcgct cgccggcgcc gggcggcgcg gtgtaccgcg gggtcgtgtt
     1021 gccggagcct gacccttcgg acatgtccga cgtgcgcctc gacctctcga tcgacgccgt
     1081 caacgcgctg ctgcacgcct ggaccgccaa tgggctgctc gccgaccgca tggcggcggc
     1141 gcagtgggtc gggcaggtcg atcgcgcgtt gcaggagtgg acgctgctgt cgctggtcgg
     1201 cgtcacggtg acgcgggcgc cggtgctgat ggccgccgat cagggcaacg cggccccgag
     1261 gcgcgacgcg tgggcgctga gcttcggcgg gctcgtgctc gacctgcgcg gaggtgagca
     1321 tgccggcacg gtggtcgccg ccgggcgcgg gtggatcacg cccgagctcg acgccggcag
     1381 cgggcggctc gagctcggcg gcgtggtcga tcgcctgcgc ctgacctgca tcgagggaca
     1441 ggtcctcacg ccctgcttcg gggcgctgct cgagctcggc gaggtcgagt cgcggctcga
     1501 cgcgagcctg gcccccggga caggtcggtt gccgggtatc gatgttcgcg ggctgctacg
     1561 gacgcgaacc cgggcgctgc gtccggaggg cctcgacgtc gaggagctca cggtgacggt
     1621 gcccgcgggg cacccggggg tgctgcgcgt gcgggcgcgg ctgcgtccgt gatccgcctg
     1681 cactcgcag
//
LOCUS       DASWNJ010000170         3465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_109398, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000170 DASWNJ010000000
VERSION     DASWNJ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109398"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="VGB85_03140"
     CDS             <1..836
                     /locus_tag="VGB85_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133041.1"
                     /translation="RSRRGAAPMNLEGARVVLRPRTQSEIFDLAFRFCAALALPLYLR
                     LAAIVLLPCLLACGYVHRLSDGEWPVTWAVALALGAFAQSVFTVAASRLLFEPHPRVL
                     GVVLTTLRRLPALFGALLLHACLLGAGAFVAIGSFFVWVWHTYLYEAVLLEQQGPIGA
                     ISRASALIKHKFGAALVTCIGGATVVALGILSAELLLGSIVEWVLQLGEPFGALYPDG
                     GSPYALIGYFAALPLVATARFLSYIDGRTRQDGWDIQLRFMSIAAGDPDPDATSEEAT
                     P"
     gene            833..2878
                     /locus_tag="VGB85_03145"
     CDS             833..2878
                     /locus_tag="VGB85_03145"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEY0133042.1"
                     /translation="MISAALLFTLSLVAAPSYESAKEQTRKILADDGYGFCNDPKFPL
                     TDAEARWCPPVGPDKNPRCPAFPSACQAPRAVLQDLGRLDFSRGGEGKSDVGTDPPPA
                     KDDPSEDDKPGETKSGKRKDPRRETYADPTAQMPELGGLGYILFWVILGGGVLALVLA
                     VVSNSSRYKPEDEVPTSPPDTDAVPAPARPGAQDQLVRDVGSLLEQARAAASAGDYGR
                     AIQRVHAALLHRLDHDGLIRVAPFRTNGDYVRDLAPQPDLRGSVRQIVRDVEQVQFGT
                     APADASLYERVLKRVVPIATRRGDTLVLLLAACSLFSCDPDNSYPFDRSPSGASAVIE
                     LGAAYDLEIKFRSTALADLDRSEPLDVDRGRTLVLLHDAPDPTASEWDRLLDWADNGH
                     HLVLAGLPPPSELNLHIETGASGHVLRVSDGFDLGETDPLDLLTADDARLVRDNTDGT
                     PLLEHADGELYAVQYERGYRGGDITVFADDRLFTNGGLMLRDNPEFLVRFLKDLGGKE
                     VELVDGLLDLGADTPAEAIANTRLTPVILQLLALMAILFFWRGVRFGRPHDPPSRSRR
                     HFSEHIEALGTHYARARASRHAVRLYAAWALERLREKTQSTRTPGLYPLAQAIAGRTG
                     DDESRVMETLVEASSLQGDAGDPRIAYSRKAVAGANLDDLRLMQDLARLVRLVGGPR"
     gene            2875..>3465
                     /locus_tag="VGB85_03150"
     CDS             2875..>3465
                     /locus_tag="VGB85_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0133043.1"
                     /translation="MTPHNFAEICTNIRNQVGRVLLGQDEIVDSVLIGVLARGHVLIE
                     GVPGLGKTLMVRALAKVLGCVAKRIQFTPDLMPSDVTGGNIFNQQKQQFEFLPGPVFT
                     QLLLADEINRAPAKTQAALLEAMADRSVSSDGVTRPLPQPFFVMATQNPVESQGTYPL
                     PEAQLDRFLMKLDVTHPSRETEKRLLANVVKGFDAAA"
BASE COUNT          484 a         1437 c         1037 g          507 t
ORIGIN      
        1 ctcgaagtcg tcggggggcc gcgcccatga acctcgaggg cgcccgcgtg gtcctgcgcc
       61 cgcgcacaca gtcggagatc ttcgacctcg cgtttcgctt ctgcgccgcg ctcgcgctgc
      121 cgctgtacct gcgcctcgcc gcgatcgtgc tgctgccctg tctgctcgcg tgcggctacg
      181 tgcacaggct cagcgacggc gagtggccgg tcacctgggc cgtcgcgctc gccctcggcg
      241 cgttcgccca gagcgtcttc accgtcgccg cctcgcggct gctgttcgag ccccacccgc
      301 gcgtgctcgg cgtcgtgctg acgaccctgc gccgcctccc ggccctgttc ggcgcgctgc
      361 tgctgcacgc ctgcctgctc ggcgccggcg ccttcgtcgc gatcggcagc ttcttcgtct
      421 gggtctggca cacctatctg tacgaggccg tcctgctcga gcagcagggt cctatcgggg
      481 cgatcagccg cgcctccgcc ctcatcaagc acaagttcgg ggccgccctc gtcacctgca
      541 tcggcggcgc caccgtcgtc gcgctcggca tcctctccgc ggagctcctg ctcggcagca
      601 tcgtcgagtg ggtgctccag ctcggcgagc cctttggcgc cctgtacccc gacggcggct
      661 cgccctacgc gctgatcggc tacttcgccg cgctgccgct cgtcgccacc gcccgcttcc
      721 tcagctacat cgacggccgc acgcgccagg acggctggga catccagctg cgcttcatga
      781 gcatcgccgc cggcgacccc gaccccgacg ccacgagcga ggaggccacc ccgtgatctc
      841 cgcggcgctc ctgttcaccc tctcgctcgt cgccgcgccc tcctacgagt ccgccaagga
      901 gcagacgcga aagatcctcg ccgatgacgg ctacgggttc tgcaacgacc cgaagttccc
      961 gctgaccgac gccgaggccc gctggtgccc gcccgtcggc cccgacaaga acccgcgctg
     1021 ccccgccttc ccgtccgcct gccaggcccc gcgcgcggtc ctccaggacc tcggccgcct
     1081 cgacttctcg cgcggcggcg agggcaagtc cgacgtcggc accgacccgc cccccgccaa
     1141 ggacgacccg agcgaggacg acaagcccgg cgagaccaag tccggcaagc gcaaggatcc
     1201 caggcgcgag acctacgccg acccgaccgc gcagatgccc gagctcggcg gcctcggcta
     1261 tatcctgttc tgggtgatcc tcggcggcgg cgtgctcgcg ctggtcctcg ccgtcgtcag
     1321 caacagcagc agatacaagc ccgaggacga ggtcccgacc tcgccgccgg acaccgacgc
     1381 cgttcccgcc cccgcgcggc caggcgcgca ggatcaactc gtgcgcgacg tcggctcctt
     1441 gctcgagcag gcgcgcgcgg ccgccagcgc cggtgactac ggccgcgcga tccagcgggt
     1501 gcacgccgct ctgctgcatc gcctcgatca cgacggcctg atccgcgtcg ccccgtttcg
     1561 caccaacggc gactacgtcc gcgacctcgc cccgcagccc gacctgcgcg gcagcgtccg
     1621 ccagatcgtc cgcgacgtcg agcaggtcca gttcggcacc gcccccgccg acgcctcgct
     1681 ctacgagcgc gtcctcaagc gcgtcgtccc gatcgccacc cgccgcggcg acacgctggt
     1741 gctgttgctc gccgcctgct cgctgttctc ctgcgacccc gacaactcct accccttcga
     1801 ccgctcgccc tccggcgcca gcgcggtgat cgagctcggc gccgcctacg acctcgagat
     1861 caagttccgc agcaccgccc tcgccgacct cgaccgcagc gagcccctcg atgtcgaccg
     1921 cggccgcacc ctcgtcctcc tccacgacgc ccccgacccg accgcgagcg agtgggaccg
     1981 cctgctcgac tgggccgaca acggccacca cctcgtgctc gccggcctcc cgccgccctc
     2041 cgagctcaac ctgcacatcg agacaggtgc ttcgggacat gtcctcagag tcagcgacgg
     2101 cttcgacctc ggcgagaccg acccgctcga cctcctcacc gccgacgacg ccaggctcgt
     2161 gcgcgacaac accgacggca cgccgctgct cgagcacgcc gacggcgagc tgtacgccgt
     2221 gcaatacgaa cgcggctacc gcggcggcga catcaccgtg ttcgccgacg accgcctgtt
     2281 caccaacggc ggcctgatgc tgcgcgacaa ccccgaattc ctggtccgct tcctcaagga
     2341 cctcggtggc aaggaggtcg agctcgtcga cggcctgctc gacctcggcg ccgacacccc
     2401 ggccgaggcg atcgccaaca cccggctgac ccccgtcatc ttgcagctgc tcgccctgat
     2461 ggcgatcctg ttcttctggc gcggcgttcg tttcggtcgc ccgcacgacc cgccgagcag
     2521 gtcgcggcgc cacttctccg agcacatcga ggcgctcggc acccactacg cccgcgcccg
     2581 cgcctcccgc cacgccgtgc gcctctacgc cgcctgggcg ctcgagcgcc tgcgcgagaa
     2641 gacccagtcc acgcgcacgc ccggcctgta tccgctcgcg caggcgatcg ccggccgcac
     2701 cggcgacgac gagagccgcg tcatggagac cctcgtcgag gccagctcgc tgcaaggcga
     2761 cgctggcgac ccgcggatcg cctacagccg caaggccgtc gccggcgcca acctcgacga
     2821 cctccgactc atgcaagacc tcgctcgcct cgttcgcctc gtgggaggac cccgatgaca
     2881 ccgcacaact tcgccgagat ctgcaccaac attcgtaatc aggtcggccg cgtcctgctc
     2941 ggccaggacg agatcgtcga ctccgtcctg atcggcgtgc tcgcccgcgg tcacgtgttg
     3001 atcgaaggcg tgcccggcct cggcaagacc ctcatggtcc gcgccctcgc caaggtgctc
     3061 ggctgcgtcg ccaagcgcat ccagttcacc ccggacctga tgcccagcga tgtcaccggc
     3121 ggcaacatct tcaatcagca aaagcagcag ttcgagttct tgcccgggcc ggtgttcacc
     3181 cagctgctgc tcgccgacga gatcaaccgc gcccccgcca agacccaggc cgcgctgctc
     3241 gaggcgatgg ccgaccgcag cgtctcctct gacggcgtca cccggccgct gccccagccc
     3301 ttcttcgtca tggcgacgca gaaccccgtc gagtcgcagg gcacctaccc gctgcccgag
     3361 gcccagctcg accgcttcct gatgaagctc gacgtcaccc acccgagccg cgagaccgag
     3421 aagcgcctgc tcgccaacgt cgtcaagggc ttcgacgccg ccgcg
//
LOCUS       DASWNJ010000171         1852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_427270, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000171 DASWNJ010000000
VERSION     DASWNJ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427270"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1145)
                     /locus_tag="VGB85_03155"
     CDS             complement(138..1145)
                     /locus_tag="VGB85_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133044.1"
                     /translation="MTTLRLSPALVLLSALTACGGGDTKDAKKTEAKTEARAEVADSK
                     ADAKVRPRARATDEARMPAPKGRVVALGGLDGNLAATKAALKAAGAIDDAGNWTGGDL
                     VVVQLGNQLGPGKDEQAVLRLLDTLAGQASQAGGALYRLAGANEILNVALTFDGVSAQ
                     GFKDYAGEKVNAEDPRIDGLPKPQRGRATAFAAGGSMAKKLAEQKLVLIVGDTVFAHA
                     GVLEDHVKYGLDIINTEGKKWMLAQEAMMPNMLSDLGPARTPKLRGPQPFCGGVEQVL
                     GDLAAKRMVITRGPEKSEATMCDGLLLRAGPVAGDASGGPEIVEIAGDAAKFVPVAGG
                     K"
     gene            1386..>1852
                     /locus_tag="VGB85_03160"
     CDS             1386..>1852
                     /locus_tag="VGB85_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEY0133045.1"
                     /translation="MSIRLLAAPLLAVCFTSLAPGQAAAAPASSPVPKVSVEKFTLPN
                     GLRVVLSEDHSAPVVTVYTTFRVGGAHEVKGSTGFAHLFEHMMFNGSRNVPEGAYAYY
                     VDATGGDVNANTEHDRTNYYQTVPGNYLEGVLYLESNRMANLEISERALDNEKA"
BASE COUNT          248 a          648 c          659 g          297 t
ORIGIN      
        1 tgccgcagta gcggcaggcg acgctcgtcg cgtcggcggc gatgtgcacc ggggcggcgc
       61 acttcgggca ctggatgtcg atcagcttcg ccatgcgtgg cgccgaggat aactcagctt
      121 ccgcgcgcgg cgagggctca cttgccgccc gcgacaggca cgaatttggc cgcgtccccg
      181 gcgatctcga cgatctccgg gccgccgctc gcgtcaccgg cgaccgggcc ggcgcgcagc
      241 agcaggccgt cgcacatcgt cgcctcactc ttctcggggc cgcgcgtgat caccatgcgc
      301 ttggccgcga ggtcgccgag gacctgctcg acgccgccgc agaacggctg cgggccgcgc
      361 agcttgggcg tgcgcgcggg gccgaggtcc gagagcatgt tgggcatcat cgcctcctgc
      421 gcgagcatcc actttttacc ctcggtgttg atgatgtcga ggccgtactt gacgtggtcc
      481 tcgagcacac cggcgtgggc gaacacggtg tcgccgacga tcagcaccag cttctgctcg
      541 gcgagcttct tcgccatgct gccgcccgcg gcgaaggcgg tcgcgcggcc gcgctggggc
      601 ttgggcaggc cgtcgatgcg cgggtcctcg gcgttgacct tctcgccggc gtagtccttg
      661 aacccctgcg cgctgacgcc gtcgaaggtc agcgcgacgt tgaggatctc gttggccccg
      721 gcgaggcggt acagcgcgcc gccggcctgg ctcgcctgcc cggcgagcgt gtcgagcagg
      781 cgcagcacgg cctgctcgtc cttgcccggc ccgagctggt tgccgagctg cacgacgacg
      841 aggtcaccgc cggtccagtt gccggcgtcg tcgatcgctc ccgcggcctt gagcgcggcc
      901 ttcgtcgccg cgaggttgcc gtcgaggccg ccgagcgcga cgacccgacc cttcggcgcc
      961 ggcatgcgcg cctcgtcggt cgcgcgggcg cgcgggcgca ccttggcgtc cgccttgctg
     1021 tccgcgacct cggccctggc ctccgtcttg gcctccgtct tcttggcgtc cttggtgtcg
     1081 ccgccgccgc aggcggtcag ggcactgagc agcacgaggg caggcgacag gcggagcgtg
     1141 gtcatgggcg catgatacag aaagatacag aaagaacaga aagtcgagcc cggctccgcg
     1201 ccgtcgagcg gccgcgggcg gcctcgtgcg taaacgcgcc gtttcgccgg gggcgcacgc
     1261 gagggccgtg aaaccagggt acagggcagc gggtcacggg aggtcggcct ggcggtgccg
     1321 ggccggtgct tgtcctgcga ggctcgcctc gcctaccgtg ccgcgtgtcg ctggaggtcc
     1381 ctcccatgtc cattcgtctg ctggccgcgc ccttgctggc cgtatgtttc acctcgctcg
     1441 cgcccgggca ggccgccgct gcgccggcct cgtcgccggt gccgaaggtg tcggtcgaga
     1501 agttcacgct gccgaacggc cttcgcgtgg tcctcagcga ggaccacagc gcgccggtgg
     1561 tgacggtgta cacgaccttt cgtgtcggcg gcgcgcacga ggtcaagggc tcgaccggct
     1621 tcgcgcacct gttcgagcac atgatgttca acggctcgcg caacgtgccc gagggggcat
     1681 acgcgtatta tgtcgatgcg accggcggcg acgtcaacgc caacaccgag cacgaccgca
     1741 cgaactacta tcaaacggtg cccggcaatt acctcgaggg cgtgctctac ctcgagagca
     1801 accggatggc gaacctggag atcagcgagc gcgcgctcga caacgagaag gc
//
LOCUS       DASWNJ010000172         2093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_144382, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000172 DASWNJ010000000
VERSION     DASWNJ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144382"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2093)
                     /locus_tag="VGB85_03165"
     CDS             complement(<1..>2093)
                     /locus_tag="VGB85_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0133046.1"
                     /translation="ESALATLTADEGGERATHYQAVQRAIADLGAGVDLLADGSVIAT
                     VFPSASAVDQALVGARCGLVMRELLPDHAVAVTTGRGVLRGEAPIGAAIDRALHLLAE
                     RTTLAVQGVWLDGLTRDLLAGRLRIAEHDGHPLLLPEQDATANDGVRRLLGKLTPCVG
                     REHELASLAALLDACIDEPAARAVGVVAPPGMGKSRVRHELVRAALRRHPDLLVVLGR
                     CDLMQAGAPYGPLADALRRLCGLCGDEPPATARASLRARTGLHLPAEAAGRVATFLGE
                     LCRIPAADDPSPALHAARNDPKMMRDQVLRAFVDLLRGELQHAPMLLILEDLHWGDVL
                     SVGLVEQALRELGELPLCVLVLARPEAQELFPTFWTELHVLPLRPLARRACERLALEI
                     LRPALGDDLDPEAVARIVEQAAGHPLYLEELVRAVAEGRHDTLPETVLAMLVARLSQL
                     DPGARQVLRAASVFGESFTQSDLAALLGDVSGLDASLAVLVRGEFVQSAREGWDGAER
                     HKFRHALLRDAAYSLLTAEDRRAAHRLACAHLEAMGAEPAALAEHAHAAEDRALAARY
                     HVEAAGAALASFDLDAARLQCERALACGLDGAPLGTVRAIQAWVAYFHLDQQTGYEHA
                     LAAVDALPPGSLWWTRALQVCFYSFFMLGRVEHVERLIAAFVAAEPELDARAVYVESG
                     AVLMTFFGTCGLRRAG"
BASE COUNT          279 a          759 c          828 g          227 t
ORIGIN      
        1 cccgcgcgtc gcaggccgca ggtcccgaag aatgtcatca acacggcgcc ggactcgacg
       61 tagacggcgc gcgcgtcgag ctcgggttcg gcggcgacga aggcggcgat caggcgctcg
      121 acgtgctcga cgcggccgag catgaagaac gagtagaagc agacctgtag ggcgcgggtc
      181 caccacaggc tgcccggcgg caaggcatcg accgccgcga gcgcgtgctc gtagccggtc
      241 tgctggtcga ggtgaaagta ggccacccac gcctggatcg cgcggacggt gccgaggggc
      301 gcgccgtcga ggccgcaggc gagggcacgc tcgcattgca gccgggccgc gtcgaggtcg
      361 aagctcgcga gcgccgcgcc ggcggcctcg acgtggtagc gggccgcgag cgcgcggtcc
      421 tcggcggcgt gggcgtgctc ggcgagcgcc gctggctcgg cgcccatcgc ctcgaggtgc
      481 gcgcaggcga ggcgatgcgc ggcgcgccgg tcctcggcgg tcagcaggct gtaggcggcg
      541 tcgcgcagca gcgcgtggcg gaacttgtgg cgctccgcgc cgtcccagcc ctcgcgtgcg
      601 ctctggacga actcgccgcg gacgaggacc gcgagcgatg catcgagccc ggagacgtcg
      661 ccgagcagcg ccgcgaggtc gctctgcgta aaggactcgc cgaacacgct cgcggcgcgc
      721 agcacctgac gggcgccggg gtcgagctgc gagaggcgag cgacgagcat cgccagcacg
      781 gtctcgggca gcgtgtcgtg gcggccctcg gcgacggccc ggacgagctc ctcgaggtac
      841 agcgggtggc cggcggcctg ctcgacgatg cgggcgaccg cctcggggtc gaggtcgtcg
      901 ccgagcgcgg ggcggaggat ctcgagggcg aggcgctcgc aggcccggcg cgcgagcggg
      961 cgcaacggca ggacgtggag ctcggtccag aacgtcggga acagctcctg cgcctccggg
     1021 cgcgcgagca cgaggacaca cagcggcagc tcgccgagct cgcgtagcgc ctgctcgacg
     1081 aggccgacgg acaggacgtc gccccagtgc aggtcctcca ggatcagcag catcggggcg
     1141 tgctgcagct cgccgcgcag caggtcgacg aaggcccgca ggacctggtc gcgcatcatc
     1201 ttcgggtcgt tgcgcgcggc gtggagcgcc gggctcgggt cgtccgccgc ggggatccgg
     1261 cacagctcgc cgaggaaggt cgccacgcgt ccggcggcct cggcgggcaa gtgcaggccg
     1321 gtgcgcgccc gcaggctcgc gcgggcggtc gcgggcggct cgtcgccaca cagcccgcac
     1381 agccggcgca gcgcgtcggc gagcggaccg tacggcgcgc cggcctgcat caagtcgcag
     1441 cgcccgagca ccacgagcag gtcggggtgg cggcgcagcg ccgcgcgcac gagctcgtgg
     1501 cgcacccgcg acttccccat cccgggcggc gcgaccacgc cgaccgcccg cgcggcgggc
     1561 tcgtcgatgc acgcgtcgag cagcgccgcg aggctggcga gctcgtgctc gcggccgacg
     1621 cagggtgtca gcttgccgag caggcggcgg accccgtcat tggcggtcgc gtcctgctcg
     1681 ggcagcagca gcgggtggcc gtcgtgctcg gcgatccgca ggcgcccggc gagcaggtcg
     1741 cgggtgagcc cgtcgagcca gacgccctgc accgccagcg tcgtgcgctc ggcgagcagg
     1801 tgcaacgccc ggtcgatcgc cgcgccgatc ggcgcctcgc cgcgcaggac cccgcggccg
     1861 gtcgtcaccg cgacggcgtg gtccggcagc agctcgcgca tcacgagccc gcagcgagcg
     1921 ccgaccagcg cctggtcgac cgcgctcgcg ctcgggaaca cggtggcgat gaccgagccg
     1981 tccgccagca gatcgacccc cgcgccgagg tcggcgatcg cccgctgcac cgcctggtag
     2041 tgcgtggctc gctcgccgcc ctcgtccgcg gtcagggtcg cgagggccga ctc
//
LOCUS       DASWNJ010000173         1523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_497344, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000173 DASWNJ010000000
VERSION     DASWNJ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497344"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VGB85_03170"
     CDS             <1..774
                     /locus_tag="VGB85_03170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HEY0133047.1"
                     /translation="SWSSYYGGPGDDLPGGIAVAGDRIYVTGNTTSSSGIATANAHLD
                     QLNVEFAVDGFLVRFTQGAGHTCAGPEDCLGGQCVDGVCCDSPCDGACDVCSAALGAR
                     ADGTCTPLAADVVCRPASGACDAAELCPGDAASCPEDAAVPDGEPCDDGMCMAGACEP
                     SPGSTSTDAGTSTSGTSSGGGPGPGDSTVTPTGEAPTTGGGDSNGSGGSEGSSGSTTG
                     DPGGSGDGGCQCRTPGGPGLPGLEGLLVLAALRRRRRVV"
     gene            1023..1107
                     /locus_tag="VGB85_03175"
     tRNA            1023..1107
                     /locus_tag="VGB85_03175"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1057..1059,aa:Tyr,seq:gta)
     gene            1211..1281
                     /locus_tag="VGB85_03180"
     tRNA            1211..1281
                     /locus_tag="VGB85_03180"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1243..1245,aa:Cys,seq:gca)
     gene            1325..>1523
                     /locus_tag="VGB85_03185"
     CDS             1325..>1523
                     /locus_tag="VGB85_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0133048.1"
                     /translation="MNTKPHLNCGTIGHVDHGKTTLTAALTRVCADHFGSKARAFDEI
                     DNAPEERERGITINAAHVEYET"
BASE COUNT          253 a          526 c          510 g          234 t
ORIGIN      
        1 tcctggtcgt cctactacgg cggccccggt gacgacctcc cgggtgggat cgccgtcgcg
       61 ggcgaccgga tctacgtcac cggcaacacc acctcgtcga gcgggatcgc caccgcgaac
      121 gctcacctcg atcagctcaa cgtcgagttc gcggtcgatg gattcctcgt ccgcttcacc
      181 cagggcgccg gtcatacctg cgccggtccc gaagattgcc tcgggggtca gtgcgtcgat
      241 ggcgtgtgct gcgacagccc ctgcgatggc gcctgcgacg tctgcagcgc cgccctcggc
      301 gcgcgggccg atggcacgtg cacgccgctc gccgccgacg tcgtctgtcg accggcgagc
      361 ggggcgtgtg acgccgccga gctgtgcccg ggtgacgccg ccagctgccc cgaggacgcc
      421 gccgtccccg acggcgagcc ctgcgacgat ggcatgtgca tggccggcgc gtgcgagcca
      481 tcgcccggct cgacctccac cgacgccggc acatcgacct cgggcaccag cagcggcggc
      541 ggacctggcc ccggagacag caccgtcacg ccgaccggcg aagctccgac caccggcggc
      601 ggcgacagca acggcagcgg cggcagcgag ggcagcagtg gcagcaccac gggcgacccg
      661 gggggcagcg gtgacggcgg ctgccagtgc cggaccccgg gtgggccggg cctcccgggc
      721 ctcgagggtc tgctcgtgct cgccgcgctg cgacggcgtc ggcgagtggt gtgatgaatt
      781 ggcgcctgac atcgagcggt acacatccgc gcccgacagc gtcgattaaa aactttcaga
      841 gatactcggc gcacatatgc ttgactcgtc gcgggaccgc tggtatctcc tcggcatcat
      901 gctttgtccc gcccacaacg accagtcgaa gcgcgccggc gatgccgcgc agctcctggc
      961 gctgttgtat cgactttgat acagcggctc cgggcgagct gcaggcatgt gcacagaaca
     1021 gcgggtgatt gacccgagcg gcaaaggggg cggattgtag ttccgccgtg ctccggcact
     1081 tcgggggttc gagtccctca tcacccatcg gcgtcccatc aggaggcgcg ggcgggctga
     1141 cgaatgtcag cagctcgtgg ccgccaatat ccacatttat tcttcttttt aaagaagaat
     1201 gaatgtaacc ggtcccgtgt ccgagcggtc aggtgcacga ctgcaaatcg tgttacccgg
     1261 gttcgaatcc cggcgggacg ttgtatttcg cctggcgagc ccaggagaag tcgagaacta
     1321 tgtcatgaac accaagcccc acctcaattg cggcacgatc ggccacgtcg atcacggcaa
     1381 gaccaccctg accgcggcgc tgacccgcgt ctgcgcggac cacttcggct cgaaggcgcg
     1441 tgccttcgac gagatcgaca acgcccccga ggagcgggag cgcgggatca cgatcaacgc
     1501 ggcgcacgtc gagtacgaga ccg
//
LOCUS       DASWNJ010000174         2722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_144409, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000174 DASWNJ010000000
VERSION     DASWNJ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144409"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VGB85_03190"
     CDS             complement(<1..363)
                     /locus_tag="VGB85_03190"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0133049.1"
                     /translation="MNAERDLHRLLLRQLRRLGISEQGAPPSAEAWRALLAAVSSTYA
                     SDEQDRYLLERSLDLSSREMQVLYENLREVSETRIAAERDRLRSVLAALSHGLCVLDR
                     AGMVESANAAALALLGADE"
     gene            complement(353..1495)
                     /locus_tag="VGB85_03195"
     CDS             complement(353..1495)
                     /locus_tag="VGB85_03195"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEY0133050.1"
                     /translation="MQLRSVRLDGSGSGSAVPVDLDSEQTLVLVFGAASLLDSPGPVV
                     DLVSAFPRSKVLGCSTAGEIFGAKVGDDSLVVAVARFAATTLATASAPISAAEQSHAA
                     GEAIGRALARPDLKGVHVLSDGLVVNGSELVRGLVEVLPSGVVVTGGLAGDGDRFART
                     WVLHAGAPASGHVTAVGFYGDRVQIGHGSAGGWDPFGPRRLVTRSKGNILYELDGKPA
                     LELYKQYLGDRAQGLPATGLLFPLALSREGADGEDRLVRTILAVDEATQSLTFAGDIP
                     TGAHAQLMKAQLDRLVEGAEQAAAHARLAGGDEQEDQLAIAISCVGRRLVLRERSEEE
                     VEAIREALGASAQVVGFYSYGELSPSASGACDLHNQTMTLTTIRER"
     gene            complement(1582..2274)
                     /locus_tag="VGB85_03200"
     CDS             complement(1582..2274)
                     /locus_tag="VGB85_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY0133051.1"
                     /translation="MSLRPEQILHRDDALVVVDKPAGLVVHRGWADDEVALLGLVRDA
                     VGAWVYPVHRLDRGASGVLVFALSSAMARALQEQWTAGAVHKRYLAIVRGAPPDAHEI
                     DNPVPRSEDGPRVPAVTVIRTLHRAGRYAVVAAAPRTGRLHQIRRHLKHISCPLIGDV
                     RYGKGEHNRLFRERHDLHRLALHARSLRLHHPVTGEPLLLTAPVPDDLRRAMTGLGAP
                     ASLFEDVLDDMS"
     gene            complement(2307..2663)
                     /locus_tag="VGB85_03205"
     CDS             complement(2307..2663)
                     /locus_tag="VGB85_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133052.1"
                     /translation="MQRGRRIDVSTVLRGLLGAAAAGLIAGACASGGAGRCPQQRWCA
                     SATEASAVAEPATGTTFTCPIGLHAGVAREAGQQPPPQLPPDDRVTLDERATQARRNA
                     GDATTCCYAWTEPCKK"
BASE COUNT          371 a         1080 c          924 g          347 t
ORIGIN      
        1 ctcgtcggcg ccgagcagcg cgagcgcggc cgcgttggcc gactcgacca tgcccgcgcg
       61 gtcgagcaca cacagcccgt ggctcagggc cgcgagcacc gacctcaggc ggtcgcgctc
      121 cgccgcgatc ctcgtctcgg agacctcgcg caggttttca taaagcacct gcatctcgcg
      181 cgacgacagg tcgagcgatc gctcgagcaa gtagcgatcc tgctcgtcgc tcgcgtaggt
      241 gctgctgacg gccgccagca gggcgcgcca cgcctccgcc gagggcggcg ccccctgctc
      301 ggagatcccc agccgtcgca gctgccgcag cagcagccgg tgcaggtcgc gctcagcgtt
      361 cacggatcgt cgtcagcgtc atcgtctggt tgtgcagatc gcaggccccg gacgcgctcg
      421 gggacagctc gccgtacgag tagaagccga ccacctgggc gctcgccccc agcgcctcgc
      481 ggatcgcctc gacctcctcc tcgctgcgct cgcgcagcac cagtcggcgg ccgacgcagc
      541 tgatcgcgat cgccagctga tcctcctgtt catcaccgcc cgcgaggcga gcgtgagccg
      601 cggcctgctc ggcgccctcg accaggcggt cgagctgcgc cttcatcagc tgcgcgtgcg
      661 cgccggtcgg gatgtcgccg gcgaaggtca ggctctgcgt cgcctcgtcg accgcgagga
      721 tcgtgcgcac cagccggtcc tcgccgtcgg cgccctcgcg tgacagcgcc agcgggaaca
      781 gcagaccggt cgccggcagg ccctgcgcgc ggtcgccgag gtattgcttg tacagctcga
      841 gcgccggctt accgtcgagc tcgtacaaaa tattgccctt gctgcgcgtc accagccgcc
      901 gcggcccgaa cggatcccag ccgcccgcgg agccgtgccc gatctgcacg cggtcgccat
      961 aaaatccgac cgccgtcacg tggcccgagg ccggcgcgcc ggcgtgcagc acccacgtgc
     1021 gcgcgaagcg gtcgccgtcc cccgcgaggc cgcccgtcac caccacgccg gacggcagca
     1081 cctcgaccag cccgcgcacc agctcgctgc cgttgacgac cagcccgtcc gacagcacgt
     1141 gcacaccttt gagatccggc cgcgccagcg cgcggccgat cgcctcgccg gccgcgtgcg
     1201 actgctcggc cgcgctgatc ggcgcggagg cagtcgccag cgtcgtcgcc gcgaagcggg
     1261 ccaccgcgac caccaggctg tcgtcgccga ccttggcccc gaagatctcg cccgccgtcg
     1321 agcagccgag caccttcgag cgcgggaacg ccgacacgag atcgaccacc ggcccggggc
     1381 tgtcgagcag gctcgccgcg ccgaacacca gcaccagcgt ctgctccgag tcgaggtcca
     1441 ccggcacggc cgaccccgac cccgaaccat ccaaccgcac gctgcgtagc tgcaccgctc
     1501 acctcctcgc aggccgcaca gtacccgacc cgcgcccggc cacaccaggg ggaccccccg
     1561 cgcggaccga ggacatgtcc ctcaggacat gtcgtccagg acatcctcga acaggctcgc
     1621 tggcgccccg agcccggtca tcgctcgccg caggtcgtcg ggcacggggg ccgtgagcaa
     1681 caggggctcg cccgtgacag gatggtgcaa gcgcagggac cgggcgtgca gggccaggcg
     1741 gtgcaggtca tggcgctcgc ggaacagccg gttgtgttcg cccttgccgt agcgcacgtc
     1801 gccgatcagc gggcagctga tgtgcttgag gtggcggcgg atctggtgca ggcgaccggt
     1861 gcgcggcgcg gccgcgacca cggcgtagcg gccggcccgg tgcagcgtgc ggatcaccgt
     1921 caccgctggc acgcgcgggc cgtcctcgct gcgcggcacc gggttgtcga tctcgtgcgc
     1981 gtccgggggc gccccgcgga cgatcgccag gtagcgcttg tgcaccgcgc ccgccgtcca
     2041 ctgctcctgc agcgcgcgcg ccatcgccga cgacagcgcg aataccagca ccccgctggc
     2101 cccgcggtcg aggcgatgca ccgggtacac ccacgccccg accgcgtcgc gcaccagccc
     2161 gagcagcgcg acctcgtcgt ccgcccagcc gcgatgcacg accagcccgg ccggcttgtc
     2221 gaccaccacc agtgcgtcgt cgcggtgcag gatctgctct ggcctgaggg acatgtcccc
     2281 ggggccaggc tcctccgcgg cgccgctcac ttcttgcacg gctcggtcca cgcgtagcag
     2341 caggtggtcg cgtcgccggc gttgcgcctg gcctgggtcg cccgctcgtc gagcgtcacg
     2401 cggtcgtcgg gtggcagctg cggcggcggc tgttgcccgg cctcgcgggc gaccccggcg
     2461 tgcaggccga tcgggcaggt gaaggtcgtg ccggtcgccg gctcggcgac cgcgctcgcc
     2521 tccgtcgccg acgcgcacca gcgctgctgg gggcagcgcc ccgcgccccc gctcgcgcag
     2581 gcccccgcga tcaggcccgc cgcggcggcg ccgagcagcc ctcgcaagac agtagaaacg
     2641 tcgatccggc ggcctcgctg catcggcgca ccctatcatg cgcagacgcg gtccgtcagc
     2701 gacagacccc tcgacagacc cc
//
LOCUS       DASWNJ010000175         5611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_427283, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000175 DASWNJ010000000
VERSION     DASWNJ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5611
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427283"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VGB85_03210"
     CDS             <1..579
                     /locus_tag="VGB85_03210"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEY0133053.1"
                     /translation="AEAGTRTWLRSAPVDRGHAELPDFDLLELRVGSATTQPLAVDPE
                     QFTAELEPLPVSAATPVRTFRLGEDLDHPNGPRFSINDELWPFNTPIVAALGDLEIWS
                     LENDGDGDHPFHLHGVFFQVLDRDGVPEPHLAWKDTVRIGPHETLRLAVRHETPGMWM
                     YHCQIPEHAEGGMTGDLMVSDPQGAHAKEHEL"
     gene            864..1670
                     /locus_tag="VGB85_03215"
     CDS             864..1670
                     /locus_tag="VGB85_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133054.1"
                     /translation="MTSATDTDTGTGCAGAPVFETDIIPILNASCGANDNACHSRAAY
                     AADSKADCRGWLALENESLGSVYYAGPTEGQPTGCSDMELYMRLTTLDAWQCEQFDPR
                     VRYVVPCKPEESYLIHKVDGGPLCELSEGVPSQPMPLGKPLPPGELETLKAWIAAGAP
                     RVGDACPIDCDGGVDPGPQDPVAKINHPGDGEVRKVNVDIPFVGLADDPQDGKVPADK
                     LIWTSDLEGQIGVGENFNAPLSMVGKHTITLTATDIDGNEGTASLVLNME"
     gene            1689..2168
                     /locus_tag="VGB85_03220"
     CDS             1689..2168
                     /locus_tag="VGB85_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEY0133055.1"
                     /translation="MLHRHIVSITLASTLLAAGCGEDHKTTDTTTHGDTHLGGTTSED
                     TAPTTGEATSGATDESTGATSAAIEPPAAPTDLTATILEGGVHVTWKDVADNEDNYIL
                     ENKADGEAEFSVVIEMPFDSVTYHDIDVTAGTGYTYRVKAANAGGESVSNEVMVQVP"
     gene            complement(2286..3335)
                     /locus_tag="VGB85_03225"
     CDS             complement(2286..3335)
                     /locus_tag="VGB85_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY0133056.1"
                     /translation="MAIAPQRLGPVLGLLAALSFGVSAPLAKLLLAEVSPQLLAGLLY
                     LGAGAGLLLYRLVRPGTREAPLTRADLPPLLGVVALGGVAGPLLLLLGLARVSGTAGA
                     LLLNLEAPLTIVLAAVLFGEHLGRRATLATICVLLGAVVLRVTPGEVVVDSLGVAAIA
                     AACLCWALDNNLTQRLSLKDPFQIVRIKCLAAGAFNTALAFVLGASFPGPRFVALAMA
                     LGVLSYGVSVVLDAYALRLHGAAREAAYFATAPFVGAAAAVPLLGDSLGIAELGAMAL
                     MAAGVVLLMRERHAHEHVHEPMEHEHLHVHDEHHQHEHTANDLPGEPHAHPHRHPKII
                     HDHPHVPDVHHRHQH"
     gene            complement(3336..>5611)
                     /locus_tag="VGB85_03230"
     CDS             complement(3336..>5611)
                     /locus_tag="VGB85_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0133057.1"
                     /translation="SYNGAPAVVLGIQKQPGANSLELTTRIDQVLGELEGTLPRGMQI
                     ERGIFRQADFIQRSVDNVTVALRDGVILVLLIVLVFLWSLRATAITVVAIPLSLLVAC
                     LCLHAFGATINTMTLGGLAIAVGELVDDAIIDVENVMRRLRENAAAPPEARRPTLAVV
                     LDASREIRGSIVYATLVILLAFIPLFFLSGVEGRLLQPLGLAYVVSLTASLVVALTVT
                     PVLCSLLLRRVRADAEHHDSRLVTTLKGLYRPVLRAAVRGWRVVMAVALAGLVAAVVA
                     AASLGRAFLPEFNEGTLTVSAVTLPGTSLTQSNEIGAWIERILLRRPEVTSVARRTGR
                     AELDEHAQGVNAAELDVNLAPDLPDREAFLAELRADLSAVPGTFIVLGQPISHRIDHM
                     LSGTRANIAIKIFGDDLYELRRVAARVEAAVADVPGLVDLSTEQQTDIPQITVRLDRA
                     ALARHGLRTADVARTMETAFTGRSVSQVLEGQRSFDLVVKLDPAALADLEALRRTPIR
                     ATDGSVLPLAALARIDRDLGPNTISREQVQRKIVVMGNVAGRDVVSVVGDIQQRLAAQ
                     DPLPEGYRVEYGGQFESAEAASSTLLALGLMVIAGIFVLLYSAFKTLRDAAIVLLNLP
                     FALIGGVLGVVLGGGVLSVASLVGFITLFGIATRNGIMLVAHIQHLLRETPGVPVRDA
                     VEQAALERLAPILMTALAAGLGLVPLALSAGVPGSEIQAPMALVILCGLASSTALNMF
                     VVPALYTRFHREAAPET"
BASE COUNT         1005 a         1887 c         1967 g          752 t
ORIGIN      
        1 gccgaagccg gcacgaggac gtggctacgc agcgcgccgg tcgaccgcgg gcacgcggag
       61 ctaccggact tcgatctcct ggagctgcgc gtcggctccg cgacgacaca gcctctggcg
      121 gtcgaccccg agcagttcac ggcggagctc gaacccctcc cggtctccgc agcgacaccc
      181 gtgcgaacct ttcgcctcgg cgaggacctc gatcatccga acggcccccg gttctcgatc
      241 aacgacgagc tgtggccgtt caacacgccg atcgtcgcgg cgctcggcga cctcgagatc
      301 tggtcgctcg agaacgatgg cgacggcgac catcctttcc accttcacgg cgtcttcttc
      361 caggtgctcg atcgcgacgg agtccccgag ccgcatctcg cctggaaaga cacggtccgc
      421 atcggtcctc acgagacgct gcgcctcgcg gtgcgccacg agacccctgg aatgtggatg
      481 tatcactgcc agatccccga gcatgccgag ggcggcatga cgggggattt gatggtctcg
      541 gacccacagg gcgcgcacgc gaaggagcat gagttatgaa cacgaaattc ttggcctgga
      601 cgatccccgt actcgacttg cttgccatcg gctgtggcga cggaccgaag tcgaccggga
      661 gcgacgcgga ggccggagca accggcacgt ctaccaacga tgcgaccagc aatccgacga
      721 cgggcccagg cagcggcacg gcaacggcca ctgggaccga gggaggcgac cccacgggtt
      781 ctccgaccgg gaacgctacc ggagacacca ccagcgatcc atccggtgac ccgtctggcg
      841 atcccaccgg cccgaccacc accgtgactt ccgcgacgga cacggacacc ggcacgggct
      901 gcgctggcgc gcccgtgttc gagacggaca tcatcccgat cttgaacgcg agctgcggcg
      961 ccaacgacaa cgcctgtcac tcgcgcgccg cctacgccgc cgactcgaag gccgactgtc
     1021 gtggttggct cgccctcgag aacgagtccc tcggatcggt ctactacgcc ggcccgacgg
     1081 aaggccagcc gaccggctgc tccgacatgg agctgtacat gcggctcacc acgctcgatg
     1141 cctggcagtg cgagcagttc gatccgcggg tccgctacgt cgtgccgtgc aagcccgagg
     1201 agagctacct gatccacaag gtagacggtg gaccgctgtg cgagctgagc gaaggcgtgc
     1261 ccagccagcc gatgccgctc ggcaagccgc tcccgcccgg ggagctcgag acactcaaag
     1321 cgtggatcgc cgcgggcgcg ccgcgcgtcg gcgatgcctg tccgatcgac tgcgacggcg
     1381 gcgtcgaccc gggtccgcag gatcccgtgg ccaagatcaa tcacccgggt gacggcgagg
     1441 tccgcaaggt caacgtcgat atcccctttg tcggactggc cgatgacccc caggacggca
     1501 aggtgcccgc ggacaagctg atctggacga gcgacctcga gggccagatc ggcgtcggcg
     1561 agaatttcaa cgcgccgctg agcatggtcg gcaagcacac gatcaccctg accgcgaccg
     1621 acatcgacgg caacgagggc accgcgtccc tcgtgctcaa catggaataa ccccccgaat
     1681 cagctctcat gcttcatcga cacatcgttt ccatcaccct ggcctccacc ctcctcgccg
     1741 ccggctgcgg cgaagatcac aagaccaccg acaccaccac gcacggcgac acccacctcg
     1801 gcggcaccac gagcgaagac accgctccga cgactgggga ggcgacctcg ggggccaccg
     1861 acgagagcac gggcgcgacg tcggctgcca tcgaaccgcc cgcggccccc accgacctca
     1921 cggccaccat cctcgagggc ggcgtccacg tcacctggaa agacgtcgca gacaacgagg
     1981 acaactacat cctcgagaac aaggccgacg gcgaggccga attttccgtc gtcatcgaga
     2041 tgccattcga ctccgtcacc taccacgaca tcgacgtgac ggcgggcacc ggctacacct
     2101 accgcgtcaa ggcggccaac gctggcggcg agtcggtctc caacgaggtc atggtgcagg
     2161 tgccgtgaga tgtcgccacg aaaccactcg ccctggtgac gcctggctcg cggcacacac
     2221 acaacgcggc gaacagccac gatagcgggc gtacccgcta ttcggccatg cctcgcgctg
     2281 tcgagctaat gttggtgccg gtgatgtacg tcgggcacat gcggatggtc atgaattatt
     2341 ttcggatgcc ggtgtggatg cgcgtgtggc tcgcccggta gatcgttcgc ggtatgctcg
     2401 tgctggtggt gctcgtcgtg gacgtggaga tgttcgtgtt ccatcggctc gtggacgtgc
     2461 tcatgtgcgt gacgctcgcg catcagcagc accacgccgg ccgccatcag cgccatcgcc
     2521 ccgagctcgg cgatcccgag cgagtcgccc aggaggggta ctgctgcggc ggctccgacg
     2581 aatggagccg ttgcgaaata tgcagcctca cgcgccgcgc catgcaagcg gagggcgtac
     2641 gcatcgagga cgacgctgac gccatagctc aggacgccga gggccatggc gagggcgacg
     2701 aagcgggggc caggaaaact ggccccgagc acgaatgcca gcgcagtgtt gaacgcgccc
     2761 gcagccaggc acttgatgcg aacgatctga aacgggtcct tgagcgagag gcgctgcgtc
     2821 aggttgttgt cgagggccca acacaggcac gccgctgcga tcgccgccac gccgaggctg
     2881 tcgacgacga cttccccggg cgtgacccga aggacgacgg ctccgaggag cacgcagatc
     2941 gttgcgagcg tcgcgcggcg gccgaggtgt tcgccgaaca ggaccgcggc caggacgatc
     3001 gtcagcggcg cctcgaggtt gagcagcagt gcgccggccg tgccgctcac gcgcgcgaga
     3061 ccgaggagca acagcagcgg tccggcgacg ccaccaagcg cgacgacacc gagcaagggc
     3121 ggcagatcgg cgcgggtcag cggcgcctca cgcgtgccag gccggacgag gcggtagagc
     3181 agcaaccccg ccccggcgcc aagatacagg aggccggcga gcagttgcgg cgagacctcg
     3241 gcgagcaaca acttcgccag cggggcgctg acaccgaagc tcagggccgc gagcaacccg
     3301 aggacgggcc cgagccgctg gggtgcgatc gccattcagg tctccggcgc tgcctcgcgg
     3361 tggaagcggg tgtagagggc gggcaccacg aacatgttga gcgcggtcga ggacgcgagg
     3421 ccacagagga tgacgagggc catcggcgcc tggatctcgc tgccggggac cccggcggac
     3481 agcgcgagcg gcacgaggcc gagcccggcg gcgagcgccg tcatcaagat cggtgcgagg
     3541 cgctcgagcg cggcctgctc gacggcgtcg cggacgggca cgccgggcgt ctcccgtaac
     3601 aagtgttgaa tgtgcgcgac gagcatgatg ccgttgcggg tggcgatgcc aaacagcgta
     3661 atgaagccga ccagcgaggc gaccgagagc acaccgccgc cgagcaccac cccgaggacc
     3721 ccgccgatca gcgcgaacgg taaattcagg agtacgatcg ccgcgtcgcg cagggtcttg
     3781 aaggcgctgt agagcagcac gaagatgccc gcgatgacca tcaagccgag cgcgagcagc
     3841 gtgctcgagg ccgcctcggc gctctcgaac tggccgccgt attcgacgcg gtagccctcg
     3901 ggcagcgggt cctgggccgc gagtcgctgc tggatgtcgc cgaccacgct gacgacgtca
     3961 cggccggcga cgttgcccat gacgacgatc ttgcgctgca cctgctcgcg gctgatcgtg
     4021 ttcgggccga ggtcgcggtc gatccgcgcc agcgcggcga gcggcaggac gctgccgtcg
     4081 gtcgcgcgga tcggcgtgcg gcgcagggcc tcgaggtcgg cgagcgcggc cgggtcgagc
     4141 ttgacgacga ggtcgaagct gcgctggccc tcgagcactt gcgagacgct gcggccggtg
     4201 aacgcggtct ccatggtgcg ggcgacgtcg gcggtgcgca ggccatggcg cgcgagcgcg
     4261 gcgcggtcga ggcggacggt gatctgcggg atgtcggtct gctgctcggt ggagaggtcg
     4321 accaggccgg gtacatcggc gacggcggcc tcgacgcggg cggcgacgcg gcgcagctcg
     4381 tagaggtcat cgccgaagat cttgatcgca atgttggcgc gcgtgcccga cagcatgtgg
     4441 tcgatgcgat gcgagatcgg ctgcccgagc acgatgaagg tgccagggac cgccgacagg
     4501 tccgcgcgca gctcagcgag gaacgcctcg cgatcgggca ggtccggggc gaggttgacg
     4561 tcgagctcgg cggcgttgac accctgggcg tgctcgtcga gctcggcacg gccggtccgt
     4621 cgggctacac tggtgacctc ggggcgcctg agcaagatgc gctcgatcca cgcgccgatc
     4681 tcgttggact gcgtcagcga ggtgcctggc agtgtcacgg cgctgaccgt cagggtgccc
     4741 tcgttgaatt cgggcaagaa ggcccgaccg aggctcgcgg ccgccacgac ggcggcgacg
     4801 agcccggcga gggcgacggc catcacgacc cgccagccgc gtaccgcggc gcgcagcacc
     4861 ggccgataga gacctttgag cgtggtgacc aggcggctgt cgtggtgctc ggcgtcggcg
     4921 cggacccggc gcaggagcag cgagcagagc acgggcgtga cggtgagcgc gaccacgagc
     4981 gaggcggtga gtgagacgac ataggcgagc ccgagcggct ggagcagccg gccctcgacc
     5041 ccggagagga agaacagcgg gatgaaggcc agcaggatga cgagcgtggc gtagacgatc
     5101 gagccgcgga tctctcggct ggcgtcgagc acgacggcga gcgtcggtcg acgggcctcg
     5161 ggcggcgccg cagcgttctc tcgcaggcgg cgcatgacgt tctcgacgtc gatgatcgcg
     5221 tcgtcgacga gctcaccgac cgcgatcgcc aggccgccga gggtcatcgt attgatcgtc
     5281 gcgccgaagg cgtgcaggca caggcacgcg accagcaggg acagcgggat cgccaccacg
     5341 gtgatcgcgg tggcccgcag cgaccacagg aacaccagga cgatcagcag gaccaggatc
     5401 acaccgtcgc gcagggcgac cgtgacgttg tcgaccgagc gctggatgaa gtcggcctgg
     5461 cgaaagatgc cgcgctcgat ctgcatgcct cgcggcaacg tgccctcgag ctccccgagc
     5521 acttggtcaa tgcgcgtggt cagctcgaga gaattcgcgc cgggttgctt ctggatcccg
     5581 agcacgaccg cgggcgcccc gttgtaggac c
//
LOCUS       DASWNJ010000176         1569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_179710, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000176 DASWNJ010000000
VERSION     DASWNJ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179710"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VGB85_03235"
     CDS             complement(<1..355)
                     /locus_tag="VGB85_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133058.1"
                     /translation="MRRSAGSTIVRSMKCSVVGARRRGRALLLGLQVTLAAVAAAPRE
                     ALAAVEDEAAGRRALDEFNRGLTMVRDGDIAGLAIAKAALVTMKAALGADHPTTQQVS
                     TTLLVTRLQVRTVRLK"
     gene            562..1518
                     /locus_tag="VGB85_03240"
     CDS             562..1518
                     /locus_tag="VGB85_03240"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HEY0133059.1"
                     /translation="MRSVGVWTAIAAVAAIMTSCKRVNDPGKIDCDTEIEYQGRVVEG
                     KVEVAKFGVAAKTSIDAVRQIDQVVERYLARWKGMCREYNAGVYSKEDYRVESRAMRE
                     KMEQLDAMLMQLANAPDSAAYQAALTAMYQKMVPSEERTTLEVALRVMAQKPGESAPT
                     LVADGASLPTGSRLSFELTPSRPAHVYLYQESPAGEIVVLFPDPRIAARNPLPAAAGL
                     RLPPPPGSFRLNDKDIGKETVHVVASLAPLPQLETTLAGQGVTPAAAACSSRGLEYDA
                     GASACEGTRGLEYDSGGASSLSAVTELGSDRIVQRFSFQHVP"
BASE COUNT          243 a          550 c          548 g          228 t
ORIGIN      
        1 gcttgagcct gaccgtgcgg acctggaggc gcgtgaccag cagggtcgtg ctcacctgct
       61 gcgtcgtcgg gtgatcggcg ccaagcgccg ccttcatggt caccagcgcc gccttggcga
      121 tcgcaagccc ggcgatgtcg ccgtctcgca ccatcgtcag gccgcggttg aactcgtcga
      181 gcgcgcggcg tccggcggcc tcgtcctcga cggccgcgag ggcctcccga ggggccgccg
      241 cgacggcggc gagcgtcacc tgcaatccca gcaacaaggc gcggccgcgt cggcgcgccc
      301 ccacgacaga gcatttcatg ctgcggacta tagtggaccc cgccgatcgc cgcatcacca
      361 tttcatagga gtgtgggcgc gcgtaaagtg ctggtaatcg gacgctgacc ggccccgggg
      421 gccgcggtaa gcttttcctt accggccagc gcgggcccgc gcccacgcga aatgcacaca
      481 gcgaggagcg agacattgac catagttttt gcgccgatcg atccgcttga cagcggcgcc
      541 gccggccgag aatcttggcg catgcgtagc gtgggcgtgt ggacggcgat cgccgcggtg
      601 gcggcgatca tgacctcttg caagcgggtg aacgatcccg gcaagatcga ctgtgacacc
      661 gagatcgagt accagggtcg cgtcgtcgag gggaaggtcg aggtcgccaa gttcggggtc
      721 gcggccaaga cctcgatcga cgcggtccgc cagatcgatc aggtcgtcga gcgctacctc
      781 gcccgctgga aggggatgtg ccgcgagtac aacgccggcg tctactccaa ggaggactac
      841 cgggtcgaga gccgggcgat gcgcgagaag atggaacagc tcgacgcgat gctgatgcag
      901 ctcgccaacg cccccgactc cgccgcctat caggccgccc tgaccgcgat gtaccagaag
      961 atggtgccca gcgaggagcg cacgaccctc gaggtggcgc tgcgtgtcat ggcccaaaag
     1021 ccaggtgagt ccgcgccgac cctggtggcc gacggcgcca gccttccgac cgggtcgagg
     1081 ctgtccttcg agctgacgcc gtcgcggccg gcccacgtgt atctctacca ggagtcgccg
     1141 gccggcgaga tcgtggtcct gttccccgac ccgcggatcg ccgcccgcaa ccccctgcct
     1201 gccgccgccg gcctgcggct gccgccgccg cccgggagct ttcgcctcaa cgacaaggat
     1261 atcggcaagg agaccgtcca cgtcgtcgcc tcgctcgcac cgctgcctca gctggaaacg
     1321 acgctcgccg gccagggcgt gacgccggcc gccgccgcct gctcgtcgcg cggactcgag
     1381 tacgacgcgg gggcgagcgc gtgcgagggc acgcgcgggc tcgagtacga cagcggtggt
     1441 gcgtcgtcgc tgagcgcggt gaccgagctc ggcagcgatc ggatcgtcca gcgcttctcg
     1501 ttccagcacg tcccgtaggc cgacgtccgc gcgctgatgc gcgcgggcgc ccatgcgtcg
     1561 acgcggcgg
//
LOCUS       DASWNJ010000177         2291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_639870, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000177 DASWNJ010000000
VERSION     DASWNJ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639870"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /locus_tag="VGB85_03245"
     CDS             <1..1458
                     /locus_tag="VGB85_03245"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY0133060.1"
                     /translation="LPKGQDQEMRIELPATGQLRGKVLDEGGKPVKDVSIAISDGVQH
                     QQKASGDDGSFHFEHVSAGEYRVTAQRGWTELKAPGASDDDVHGEKVTVANGSVAEVK
                     LVVEGNAGKITGVVRDADGAPVADAFVEATRESDSAAASAGGAVRDGRWGSFWNTPHL
                     TDQDGRFTLDDLEKGKHALRAHRKGGGEALLEHVDLGGDVVLTIASAGRMSGVVSLKG
                     GGAPEEFTVSVEDEQTGFSRNDHFFRSNGAWSLAEVPAGKYKVRVSSGPGSAEAEAVM
                     TEGKDTSDVKIELAPKVTVRGKVVDLAGAPVAGLRVMVSAPGTWSFGEGEEKLNITDA
                     QGNYEVEHAPTGQVSVNVMPRNWNDDEYGWSSMPTMIAASESVVELPPIRVAKKQVKE
                     GEVVGDLGITLKESEPGADPLARKLIVAVVRPGSPAAEGGLVPGDEIVSVNGQDVTGP
                     NAYLYGTLTRVVEGTRVDLGLGRGGGAVITAGKRP"
     gene            complement(1472..>2291)
                     /locus_tag="VGB85_03250"
     CDS             complement(1472..>2291)
                     /locus_tag="VGB85_03250"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY0133061.1"
                     /translation="AYLVCGPLTRRVEDIYPILSVLAGPDGVDTVVQDWPLADPASID
                     LRGVTVYPLGGNGRIWVRPPMRLAVQRAAEALQARGAVIGDLEGSRMRRAFPIWSAMM
                     SLSGSPPYAEILGGDQPIAVVRELARLIVGRSRHTLPALLVVALEKLSNNFSGKKHQL
                     LAVGKLLQQELEQILGPRGVLLFPPYSRPAPRHVMPMLTPFDFVCTGLFNVLEFPSTT
                     LPVLWHAGLPVGVQVIARRGNDSLAIAAAAAIEQAFGGWRRANPPLTPAPIAVA"
BASE COUNT          364 a          704 c          952 g          271 t
ORIGIN      
        1 ttgcccaagg gacaggatca ggagatgcgg atcgagctgc cggcgaccgg ccagctgcgc
       61 ggcaaggtgc tcgacgaggg cggcaagccg gtcaaggacg tctcgatcgc gatcagcgac
      121 ggcgtgcagc accagcagaa ggcgtcgggc gacgacggca gcttccactt cgagcacgtg
      181 tcggcgggcg agtatcgggt gacggcgcag cgcggctgga cggagctcaa ggcgccgggc
      241 gcgagcgatg acgacgtgca cggcgagaag gtcacggtgg ccaacggctc ggtcgcggag
      301 gtcaagctgg tggtcgaggg caacgccggc aagatcaccg gggtggtgcg tgacgccgac
      361 ggggcgccgg tcgccgacgc gttcgtcgag gcgacccgcg agtcggacag cgcggccgcg
      421 agcgccggtg gtgcggtgcg ggacggtcgc tggggcagct tctggaacac gccgcacctg
      481 accgaccagg acgggcgctt cacgctcgac gacctcgaga agggcaagca cgccctgcgc
      541 gcgcatcgca agggcggcgg cgaggcgctg ctcgagcacg tcgacctcgg cggtgacgtc
      601 gtgctgacga tcgcgtcggc ggggcgtatg tcgggggtcg tgtcgctcaa gggcggcggc
      661 gcgccggagg agttcacggt cagcgtcgag gacgagcaga cgggctttag ccgcaacgac
      721 cacttcttcc gcagcaacgg cgcgtggagc ctcgccgagg tcccggccgg caagtacaag
      781 gtgcgcgtca gctcggggcc gggctcggcc gaggccgagg cggtgatgac ggagggcaag
      841 gacacgagcg acgtgaagat cgagctggcg ccgaaggtga cggtgcgcgg caaggtcgtc
      901 gacctcgcgg gcgcgccggt ggccgggctg cgggtgatgg tcagcgcgcc gggcacgtgg
      961 tcgttcgggg agggcgagga gaagctcaac atcaccgacg cgcagggcaa ctacgaggtc
     1021 gagcacgcgc cgacggggca ggtgtcggtc aacgtgatgc cgcgcaactg gaacgacgac
     1081 gagtacgggt ggtcgtcgat gccgacgatg atcgcggcga gcgagtcggt ggtcgagctg
     1141 ccgccgatcc gcgtggcgaa gaagcaggtc aaggagggcg aggtggtcgg cgacctcggg
     1201 atcacgctca aggagagcga gcccggggcc gacccgctgg cgcgcaagct gatcgtcgcg
     1261 gtggtccgcc ccgggagccc ggcggccgag ggcggcctcg ttccaggcga cgagatcgtc
     1321 agcgtcaatg gccaggatgt caccggcccg aacgcgtacc tgtacggcac gctgacgcgc
     1381 gtggtcgagg gcacgcgggt cgacctcggg ctcggccgcg gcggcggggc ggtgatcacg
     1441 gccggcaagc ggccctgacc gaaaggacag gtcaggcgac ggcgataggc gcgggggtca
     1501 gcggcgggtt ggcgcgccgc cagccgccga aggcctgctc gatcgcggcg gcggcggcga
     1561 tcgccaggct gtcgttgccg cggcgggcga tcacctgcac gccgacgggc agcccggcgt
     1621 gccagaggac tggcagcgtg gtcgacggga actcgagcac gttgaacagg ccggtgcaca
     1681 cgaagtcgaa gggggtcagc atcggcatga cgtggcgcgg ggcgggccgg ctgtacggcg
     1741 ggaacagcag cacgccgcgc gggccgagga tctgctcgag ctcctgttgc agcagcttgc
     1801 cgacggcgag cagctggtgc ttcttgccgc tgaagttgtt ggagagcttt tcaagggcga
     1861 cgacgagcag ggcgggcagg gtgtggcgcg agcggccgac gatcaggcgc gcgagctcgc
     1921 ggacgacggc gatcggctgg tcgccgccga ggatctccgc gtagggcggg ctgccggaca
     1981 gcgacatcat ggcgctccag atcgggaagg cgcggcgcat gcggctgccc tcgaggtcgc
     2041 cgatcacggc gccgcgcgcc tgcagggcct ccgcggcgcg ctgcaccgcg aggcgcatcg
     2101 gcgggcgcac ccagatccgg ccgttgccgc cgagcgggta gacggtgacg ccgcgcaggt
     2161 cgatgctggc ggggtccgcg agcggccagt cctggacgac ggtgtcgacg ccgtcggggc
     2221 cggcgagcac ggagaggatc gggtagatgt cctcgacgcg gcgcgtcagc gggccgcaca
     2281 cgaggtaggc g
//
LOCUS       DASWNJ010000178        10513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_427287, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000178 DASWNJ010000000
VERSION     DASWNJ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 10513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10513
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427287"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..574
                     /locus_tag="VGB85_03255"
     CDS             149..574
                     /locus_tag="VGB85_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133062.1"
                     /translation="MNRTLAYSFFKRRLLILVHTKDSPDDTEWAEYTASARQWKKDIQ
                     GFLVYSAGGGPNTMQRAQLDEAISIESHPAKTAVVTVNRIARGIVTAIGWFSPGIKAF
                     GTHQLSQAIEYLGTPTADVSVVVAEVARLKKSLGIVDAA"
     gene            1010..1768
                     /locus_tag="VGB85_03260"
     CDS             1010..1768
                     /locus_tag="VGB85_03260"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0133063.1"
                     /translation="MTRLQDEETHPALGNFGREAGLAGAHVIQLDAIEAASHPEFTEY
                     FFRRQHACYSRFRSEAAAAPHRTRSSWKGVYLIVATDHTNGEIAGGVGVYERHADAPM
                     PVELAIGDSDQLQRALSRWAHDRVVELSGLWVEEHWRRTGLSSMLMLVAMAVAREREA
                     TTAVGFSHQHVLDFYQTIGLVPDHTVGDYPYPDHKYVSTFIYGDLVGLTTAPQESKEL
                     IEHFGRSLQTHRDIIWKSVPRQRTIPQPVVLSNP"
     gene            complement(1869..4073)
                     /locus_tag="VGB85_03265"
     CDS             complement(1869..4073)
                     /locus_tag="VGB85_03265"
                     /inference="COORDINATES: protein motif:HMM:NF005901.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rv1355c family protein"
                     /protein_id="HEY0133064.1"
                     /translation="MAVADTERWLPLIFDRSLAQDREDMDALIAAGHAPFVHDMIEAQ
                     VADLIRTRNPRLPLTDADSLVRAAEVIGADARDYGRWVWFPWSGRLVHVLGPEDYFEL
                     RTDRNRNKIMRHEQQKLSKLKIAIAGLSVGNAVAVTISLENLCGELRLADFDRLDLSN
                     MNRIRCGIHQLGVNKAVISARQIFEQNPYANLTLYTDGVTLDNMGSLFEDEGRKLDLV
                     IDECDSMYIKLKLREEARARRIPLLMETSDRGMLDVERYDLEPKRPILHGLLGELSAD
                     DVASLPPVARLGLILRIIGETTMSPRLAASMLEFGQTLRGLSQLGSDVVLGGATTAIA
                     VRRFGIGLPLPSGRVFIDVNKILADIQPPPVAPPDDTEAIIMRERQLTYKMVEAAAMA
                     PSLGNMQPWRFSFKGGELSLFHDVERSGGPEHVVGRENAYIAIGAALENLSIAAGGAG
                     RDAEISLFPDRTRPDMVARVRLLPRTGNRAADPLLEQIGKRTTNRKPGSRQSLSPMHA
                     QVLTAAARQSGARLQLCQDREKIGELAKILGEADRLRWFSPKLHAEFYGQIRWTAGEA
                     ARTRDGLDTRALDLDPGTRATYQLLRSYDVVKHMRDSGGGGVITMLAQMLVPTSGAVG
                     FLTIEGNGPDAFVRGGRAMQQVWLTAGALGLAVHPWTILLDMFAMLERHGGKGLDKRE
                     VSAISELREGFARCFSVSLKDTELVLFRLSQADPPTQRSLRRPVEDLLTLED"
     gene            complement(4206..5780)
                     /locus_tag="VGB85_03270"
     CDS             complement(4206..5780)
                     /locus_tag="VGB85_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY0133065.1"
                     /translation="MTDFDYDYIVVGSGFGGSVSGLRLTEKGYRVLMLEKGRELGPKD
                     FPKSNWNLKRWLWMPQLGWRGLFKMTFFRHVTVLSGVGVGGGSLVYANTLPVPKRAFF
                     QSPGWAHLADWENELAPHYKTVLRTLGAVPNPSFTPVDRIMKSVADEMGKPFEKTHVA
                     VYFGEPDQTAPDPYFGGSGPERTGCNFCGGCMLGCRFGAKNTLDKNYLYFARKRGMQL
                     LPDTEVTAVRALEGGGYRVEASTGASLLFRKKQVFTARNVVFAGGVLGSVELLLKMQR
                     DPKGLPNLSTRLGEAVRTNSESLIGVIGQKRDQDLSRGVAIGSIIQTDEHSHVEPVRY
                     PAGSNFFRTMMAPHVAGERSVVRVARMIGSIVRHPVRLARALTVSDMSKYSIILLYMR
                     TAEGTLRFRLGNLGKSMGTTVSTGKAPSATIPEATEIAEKVAARIDGLPMSMVQETVF
                     NIPTTAHILGGCCMGDSPETGVIDHQHRVFGHEGLYVIDGSAVSANPGVNPSLTIAAL
                     AERAMSFIPQNIAVQT"
     gene            5845..6252
                     /locus_tag="VGB85_03275"
     CDS             5845..6252
                     /locus_tag="VGB85_03275"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEY0133066.1"
                     /translation="MQHLTDSQRDTLRRLLEDERDALMRLRQQEATDAVAAADSVPDV
                     GDQQDAAAREAATLAHWTLADHERTRLEEIEAALRRMHDGTYGICEHTDEPISFARLE
                     IEPTARATVEAQEEHERERTREGLDASEKRRAY"
     gene            complement(6258..7397)
                     /locus_tag="VGB85_03280"
     CDS             complement(6258..7397)
                     /locus_tag="VGB85_03280"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HEY0133067.1"
                     /translation="MEITSRIETGRILVVDDEAMMRRLLEKLLKMEGYDVALASSGEQ
                     ALSELALGGADTVLLDMRMPGMSGLEVCRQIRANPRCLHTPVVFITAVNDRELRRKGM
                     EAGADDFLSKPFDEVELMARIRNSVRVKRYYDNLEQQKEQLEAAVRERTAALNDAIAR
                     LTQIQFDLRASQEETIYRLSRAAEFRDDETGQHLQRMSRYCQLIARRIGLDEATCELL
                     RIASPMHDVGKLGIPDRILLKPGRLTPDEFTVMKGHAEIGYRILVGSEATPLKMAATI
                     AHTHHEKWDGNGYPRGLKGEEIPLYGRIAAIADVFDALTSSRPYKPAWSLEDALQLMR
                     TNRGTHFDPTLIDVFFEALPEVLTIRDRFVDQPHASSPDAPPTTH"
     gene            7545..8564
                     /locus_tag="VGB85_03285"
     CDS             7545..8564
                     /locus_tag="VGB85_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0133068.1"
                     /translation="MRLPALALLLVSLPLASCDGRSSVPVPGLAPVARAPASAPVTAA
                     PSSSSSSSSSSSVPSTRAGHLPAPARLVAIGDVHGDYAATVAALRLAGAIDEQERWVG
                     GALVVVQTGDQLDRGDGERKILDLLERLQGEARSAGGALHVLNGNHELMNAAGDLRYV
                     TAGGFVDFADVPGVDTNDPRAAGALPAMRGRLLAFAPGGPYARKLAARPIIMVIGDTV
                     FAHGGVLPEHVRRGIDAINEETQKWLRGEGEISPDILQGDESPLWTRRFCQSPGPRDC
                     AVASETLTQLGVKRMVVGHTVHKDGIVNNCDGKVWCIDVGMAAYYGGHPEVLEISGDQ
                     VKPLR"
     gene            complement(8566..9408)
                     /gene="ttcA"
                     /locus_tag="VGB85_03290"
     CDS             complement(8566..9408)
                     /gene="ttcA"
                     /locus_tag="VGB85_03290"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007972.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEY0133069.1"
                     /translation="MSESTRDPGALRRALSKTMTRTLAGYNLLAEGDHVLVAVSGGKD
                     SYTLLDLLWHARARAPFKFDITAVHLDQAQPGYDGRPLAAWLESFGAPYKILREDTYS
                     VVLDHTPPGGTYCAVCSRLRRGILYTAAERLGCNKIALGHHRDDTLETLLLNLFYGGR
                     LQAMPAGYTTDDGRFQVIRPLIECAEADIAEHARNVGYPILPCNLCGSQDGLRRDAMA
                     RLLTQMEQDSPNVRAVMLSALKNVSPTHLLDTRLHARGHEKADPAAAPVQPPGPRRLP
                     VLSS"
     gene            complement(9416..9826)
                     /locus_tag="VGB85_03295"
     CDS             complement(9416..9826)
                     /locus_tag="VGB85_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEY0133070.1"
                     /translation="MTAEQIEVATRGRGILDITAQVQAIVARSGAATGLCHLFIHHTS
                     ASLLITENADPEVHRDLERFLSRLVPDGDPLFKHDDEGPDDMPAHVRTVLTQTSLAVP
                     VRDGRCDLGTWQGIYLWEHRHAGHRRRVTVTVLA"
     gene            complement(9823..10095)
                     /locus_tag="VGB85_03300"
     CDS             complement(9823..10095)
                     /locus_tag="VGB85_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HEY0133071.1"
                     /translation="MTQDNTPANVTPQQALELEAAAFRSLVAHLQDRKDVQNLDLMNL
                     AGFCRNCLSRWYQEAATAQGIELTTDQARERVYGMPYSEWKANHQR"
     gene            complement(10114..>10513)
                     /locus_tag="VGB85_03305"
     CDS             complement(10114..>10513)
                     /locus_tag="VGB85_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133072.1"
                     /translation="REVAYTVAMCHDLGIVSRDDTGPTYLARSRALFERETAGHDLKG
                     TPAQILDECMLYNHRLMPVPNLSPQADAFRRAVQIEHGRGLFRFGLDSAEIKAVFAAY
                     PRGNFDRVLLDFTWRTLTREPLTLINGIFF"
BASE COUNT         1632 a         3386 c         3782 g         1713 t
ORIGIN      
        1 cgccgtccgg agccccgctg ccgctctcgc ggtgtcggcg caggcgcagt ttcgtgcgtc
       61 cggcgagccc ggggcccgac ctcgcggcgg ggtggggatt ggggtagcat gtgccccccg
      121 tggctgggcc agcttcgagg acgccgacat gaaccgcacg ctcgcctata gtttcttcaa
      181 gcgaaggctc ctcatcctgg ttcataccaa ggactcgccg gacgacacgg agtgggcgga
      241 gtacacggcg agcgcgcggc agtggaaaaa ggacattcag ggcttcctcg tatattcggc
      301 gggtggcggt ccgaatacga tgcagcgcgc gcagctcgac gaggcgatca gcatcgagag
      361 tcacccggcg aagacggcgg tcgtcactgt caatcgaatc gctcgcggca ttgtgacagc
      421 gattgggtgg ttcagccccg gtatcaaggc atttggtacc catcaattgt cgcaggcgat
      481 cgaatatctc ggtacgccga ccgccgatgt gtccgtggtg gtcgccgagg tggcccggct
      541 caagaagtct ctcggtatcg tcgacgccgc gtaggtccgg caggtaggtc cggcaggtga
      601 gtccggcagg tgagtccggc aggtgagtcc ggggcacgta agtccaggac acacagggcg
      661 tgaggtaaat ctccgccatt ggatggagtg attgggtgac agggcagtgg tagctttggc
      721 ggttggcgat tacgccgact gccatttgtg caatccacgt tacataaatt tcaaagacat
      781 tttcattgga agttcgttga cgcgcatgga gcatcgagat taagatctcg gttccatgtt
      841 cgtttcacct gcaactgagg gttgaggcca agtcgtgtcg acatttgcag ggaatgcact
      901 aggacagagt cttcgcgacc cggggcccac ggctggccgc aatctgcaag cgcagatcgg
      961 ccagggcccc gcctctgctc ctctcctgag ccctttctgg accaaaccta tgacccgtct
     1021 tcaagatgaa gagacacacc cggcgctggg aaactttggg cgggaggcag gactggcagg
     1081 ggcccacgtc attcaattgg acgccatcga ggcggcatct catccggagt tcacggagta
     1141 cttctttcgc cggcagcacg cgtgttactc gcgattcagg tccgaggcgg ccgctgcgcc
     1201 ccatcggacg cgttcctcgt ggaagggcgt gtacctgatc gtcgccaccg atcacaccaa
     1261 tggcgagatc gccggtggcg tcggggtata tgagcgccac gcggacgccc cgatgccggt
     1321 cgagctggcg atcggtgact cggaccagct gcagcgggcg ctgtcgcgat gggcccacga
     1381 ccgggtggtc gagctcagtg gcttgtgggt ggaggagcac tggcgacgca ccggcctcag
     1441 ctccatgctg atgctggtcg ccatggcagt cgcccgggag cgcgaggcga ccaccgccgt
     1501 cggcttctcc caccagcacg tcctcgactt ctatcagacg atcggcctgg tcccggacca
     1561 cacggtaggg gattaccctt acccggatca caaatacgtc tcgaccttca tttatggtga
     1621 cctggtcggc ctgacgaccg ccccgcagga gtcgaaggag ctgatcgagc acttcgggcg
     1681 ctcgctgcag acgcatcggg acatcatctg gaagtccgtc ccgcgccagc gcacgatccc
     1741 ccagccggtc gtgctctcga atccctagag ctcgccgtag gtcgccggca cccgcccggc
     1801 ggccgtacac cgcccggggc cggccgcaca cagaaacgag tgggccggcg ccgagccggc
     1861 caaacccgtc agtcctcgag cgtcagcagg tcctcgaccg ggcgacgcag cgagcgctgg
     1921 gtcggcgggt cggcctggga cagcctaaac aacacgagct cggtgtcttt taaggagacg
     1981 ctgaagcagc gggcgaagcc ctcgcgcagc tcgctgatcg cggagacctc acgcttgtcg
     2041 aggcccttgc cgccgtggcg ctcgagcatg gcgaacatgt cgaggaggat cgtccacggg
     2101 tgcacggcca ggccgagcgc gccggccgtc agccacacct gctgcatcgc ccgcccgccg
     2161 cgcacgaagg cgtcgggtcc gttgccttcg atcgtcagga agccgacggc gccggaggtc
     2221 gggacgagca tctgcgccaa catcgtgatg acgccgccgc cgccgctgtc gcgcatgtgc
     2281 ttgacgacgt cgtaggagcg aagcagctgg taggtggcgc gggtgccggg gtcgaggtcg
     2341 agggcgcggg tgtcgaggcc gtcgcgggtc cgggccgcct cgccggcggt ccagcggatc
     2401 tggccgtaga actcggcgtg caacttgggg gagaaccacc gcaggcggtc ggcctcgccg
     2461 aggatcttgg cgagctcgcc gatcttctcg cggtcctgac acagctgcag acgggccccc
     2521 gactggcggg cggcggccgt cagcacctgc gcgtgcatcg ggctcaggct ctggcgagat
     2581 cccggcttgc ggttggtcgt gcgcttgccg atctgctcga gcagagggtc tgcggcgcgg
     2641 ttgccggtgc gcggcaggag gcggaccctg gcgaccatgt ccggtcgggt gcgatcgggg
     2701 aacagcgata tctccgcgtc acgaccggca ccccccgcgg cgatgctgag gttctcgagc
     2761 gccgcgccga tcgcgatgta ggcgttctcg cggccgacga cgtgctcggg cccgccgctg
     2821 cgctcgacgt cgtggaacag cgacagctcg ccgcccttga agctgaagcg ccacggctgc
     2881 atgttgccga gcgacggcgc catcgcggcg gcctcgacca ttttgtaggt cagctggcgc
     2941 tcgcgcatga tgatcgcctc ggtgtcgtcg ggcggggcga ccgggggcgg ctgaatgtcg
     3001 gcgaggatct tgttgacgtc gatgaagacg cggcccgacg gcagcggcaa gccgatgccg
     3061 aagcggcgca cggcgatcgc cgtggtcgcg ccgccgagca cgacgtccga gccgagctgc
     3121 gacaggccgc gcagggtctg cccgaactcg agcatcgagg cggcgaggcg cggcgacatc
     3181 gtcgtctcgc cgatgatgcg caagatcagg ccgaggcggg cgaccggcgg caagctggcg
     3241 acgtcgtccg cgctgagctc accgagcagg ccgtggagga tcgggcgctt gggctcgagg
     3301 tcgtagcgct cgacgtccag catgccgcgg tcgctggtct ccatcagcag cgggatccgc
     3361 cgcgcccggg cctcctcgcg cagcttgagc ttgatgtaca tgctgtcgca ctcgtcgatg
     3421 acgaggtcga gcttgcggcc ctcgtcctcg aacagcgagc ccatgttgtc gagcgtgacg
     3481 ccgtcggtgt agagcgtcag gttggcatag gggttctgct cgaagatctg gcgcgcgctg
     3541 atgacggcct tgttgacccc gagctggtgg atcccgcagc ggatccggtt catgttcgac
     3601 aggtcgagcc ggtcgaagtc ggcgaggcgc agctcgccgc acaggttctc gagcgagatc
     3661 gtcacggcga ccgcgttgcc gacgctcagc ccggcgatcg cgatcttcag cttcgagagc
     3721 ttctgctgct cgtgccgcat gatcttgttg cggttgcggt cggtgcgaag ctcgaagtag
     3781 tcctcgggcc cgagcacgtg caccaggcgc cccgaccacg ggaaccacac ccagcgcccg
     3841 tagtcgcggg cgtcggcgcc gatcacctcc gcggcgcgca cgagcgagtc ggcgtcggtc
     3901 agcgggagcc ggggattacg cgtgcggatc aggtcggcga cctgcgcctc gatcatgtcg
     3961 tgcacgaacg gggcgtgacc ggcggcgatc agcgcgtcca tgtcctcgcg gtcctgcgcg
     4021 agcgaacggt cgaagatcag cgggagccag cgctcggtgt cggcgacggc cacgacttct
     4081 tgccatggcg gtcggaccgt gggtgtccgc aagatcggca tcggggggtc gcagggtaac
     4141 aggcggcatc gcgcgcgcca acgaccgatc tatcctggta gtcgccgggt tttagtccgc
     4201 cgcgatcagg tctgcacggc gatgttctgc gggatgaagc tcatcgcccg ctcggcgagg
     4261 gcggcgatcg tcagcgaggg gttgaccccc gggttcgccg atacggccga gccgtcgatc
     4321 acgtagaggc cctcgtggcc gaacacgcga tgctggtggt cgatgacccc ggtctccggc
     4381 gagtcgccca tgcagcagcc gccgaggatg tgcgcggtgg tcgggatgtt gaacacggtc
     4441 tcctggacca tcgacatcgg caggccgtcg atgcgggcgg caaccttctc ggcgatctcg
     4501 gtggcctcgg ggatcgtcgc gctcggcgcc ttgccggtgc tgacggtggt ccccatgctc
     4561 ttgccgaggt tgccgaggcg aaaccgcagg gtgccctcgg cggtgcgcat gtacagcaag
     4621 atgatgctgt acttcgacat gtcggagacg gtcagggcgc gggccaggcg gaccggatgg
     4681 cggacgatcg agccgatcat ccgcgcgacg cgcaccacgc tgcgctcgcc ggcgacgtgc
     4741 ggggccatca tcgtgcgaaa gaaattcgac ccggccgggt aacgcaccgg ctcgacgtgg
     4801 gagtgctcat cggtctggat aatcgagccg atcgcgacgc cgcgtgacag gtcctggtcg
     4861 cgcttttgcc cgatcacgcc gatcagcgac tcgctgttgg tgcgcacggc ctcgccgagg
     4921 cgcgtggaga ggttcggcag gcccttcggg tcgcgctgca ttttgaggag gagctcgacc
     4981 gagccgagca cgccgcccgc gaacacgacg ttgcgcgcag tgaacacctg cttcttgcgg
     5041 aacaacagcg aggcgccggt gctcgcctcg acgcggtaac caccaccctc gagcgcgcgc
     5101 accgcggtga cctcggtgtc gggcaacagc tgcatgccgc gcttgcgggc gaagtagagg
     5161 tagttcttgt cgagggtgtt cttggcgccg aagcggcagc cgagcatgca gccgccacaa
     5221 aaattgcagc cggtgcgctc cgggccggag ccgccgaagt acgggtccgg agcggtctgg
     5281 tcgggctcgc cgaagtacac ggcgacgtgg gtcttctcga acggcttgcc catctcgtcg
     5341 gcgaccgact tcatgatgcg gtcgaccggg gtgaagctcg ggttggggac ggcgccgagg
     5401 gtgcgcagca cggtcttgta gtgcggggcg agctcgttct cccagtcggc gaggtgggcc
     5461 cagccgggcg attgaaagaa cgcgcgcttg ggcaccggca gggtgttggc gtacaccagc
     5521 gagccaccgc cgacgccgac gcccgagagc accgtgacgt ggcgaaagaa ggtcatcttg
     5581 aacaggccgc gccagccgag ctggggcatc cacagccagc gcttgaggtt ccagttggac
     5641 ttcgggaagt ccttgggccc aagctcgcgg cccttctcga gcatgaggac gcggtagccc
     5701 ttctcggtca gacgaaggcc ggagacgctg ccgccgaagc cggagcccac gacgatgtag
     5761 tcgtagtcga agtcggtcac gatggtgtca ccatatcaca tgggccctcg acggcgcccg
     5821 ggttcgcggc cacgatgggt gccgatgcaa cacctgaccg actcccagcg agacaccctg
     5881 cgccgcctcc tcgaggacga gcgtgacgcc ctgatgcggc tacgccagca agaggcgacc
     5941 gacgcggtgg ccgccgccga ttcggtgccc gacgtcggcg accagcagga cgcggcggcg
     6001 agggaagcgg cgacgctggc ccactggacc ctcgcggacc acgagcggac ccgcctggag
     6061 gagatcgaag cggcgcttcg ccgcatgcac gacgggacct acgggatctg cgagcacacc
     6121 gacgagccga tctcctttgc gcgcctcgag atcgagccga ccgcgcgggc gaccgtcgag
     6181 gcccaggagg agcacgagcg cgagcggacc cgggagggcc tcgacgccag cgagaagcgc
     6241 cgggcgtact gaggttgtca gtgtgtcgtc ggcggcgcgt cgggcgagct cgcgtgcggc
     6301 tggtcgacaa agcggtcgcg gatcgtcagc acctcgggca acgcctcgaa gaacacgtcg
     6361 atcagcgtcg ggtcgaagtg cgtgccgcgg ttggtccgca tcagctggag ggcgtcctcc
     6421 aggctccacg ccggcttgta agggcgtgag ctcgtcaggg cgtcgaacac gtcggcgatc
     6481 gcggcgatgc gcccgtacag cgggatctcc tcgcccttga ggccgcgcgg gtagccgttg
     6541 ccgtcccact tctcgtggtg ggtgtgggcg atcgtcgcgg ccatcttgag cggcgtcgcc
     6601 tccgagccga cgaggatgcg atagccgatc tccgcgtggc ccttcatcac cgtgaactcg
     6661 tcgggggtca ggcgcccggg cttgagcaag atgcggtcgg ggatcccgag cttgccgacg
     6721 tcatgcatcg gactggcgat ccgcagcagc tcacacgtcg cctcgtcgag cccgatcctg
     6781 cgggcgatca actgacagta gcggctcatg cgctgcaggt gctggccggt ctcgtcgtca
     6841 cggaactcgg ccgcgcgcga gaggcgatag atcgtctcct cctgcgaggc gcgcaggtcg
     6901 aactggatct gcgtcagccg ggcgatcgcg tcgttgagcg ccgccgtccg ctcgcgcacc
     6961 gcggcctcga gctgctcctt ctgctgctcg aggttgtcgt agtaccgctt gacccgcacg
     7021 ctgttgcgga tgcgggccat cagctcgacc tcgtcgaagg gcttgctcaa aaagtcgtcg
     7081 gcgccggcct ccatgccctt gcgccgcagc tcgcggtcgt tgaccgcggt gatgaagacg
     7141 accggcgtgt gcaagcaccg cgggttggcc cggatctgcc ggcagacctc gaggccgctc
     7201 atgccgggca tgcgcatgtc gagcagcacc gtgtcggcgc cgccgagcgc gagctcggag
     7261 agggcctgct cgccgctgct ggccagcgcc acgtcgtagc cctccatctt gagcagcttc
     7321 tccagcaatc gacgcatcat cgcctcgtca tcgacgacga ggatgcggcc ggtctctata
     7381 cgcgacgtga tctccatgga actgagccga cattaccgcg atcgccgacg cggcacgaat
     7441 cgtcgtgaca gcagatttgg cgagcagtgt gtcggacacg tcgcgcgcgc tgcgctggcg
     7501 agcgcgtatc tgcacgacgg cgcgacgctg ctaggatgcc cgcgatgcgc ctgcctgccc
     7561 tcgccctcct cctcgtctcg ctgccgctgg cgtcctgtga cggtcgatcg tccgtgccgg
     7621 tgcccggcct cgcgccggtg gcccgcgcgc ccgcgtccgc gcccgttacc gccgctccat
     7681 cttcgtcttc atcttcttca tcatcttcat ccgtcccatc gacgcgcgcg ggccacttgc
     7741 cggcgccggc ccgcctcgtg gcgatcggcg acgttcacgg cgactacgcg gcgacggtcg
     7801 cggcgctgcg tctggcgggc gcgatcgacg agcaggagcg ctgggtcggc ggcgcgctgg
     7861 tcgtcgtgca gaccggcgac cagctcgacc gcggcgacgg cgagcgaaag atcctcgacc
     7921 tgctcgagcg cttgcagggc gaggcgcgct ccgctggcgg cgcgctgcac gtcctcaacg
     7981 gcaatcacga gctgatgaac gcggccggcg acctgcgcta cgtgaccgcg ggcggcttcg
     8041 tggacttcgc cgatgtgccc ggcgtcgaca ccaacgaccc gcgcgccgcg ggggcgctgc
     8101 cggcgatgcg cgggcgcttg ctcgcgttcg cgccgggcgg cccctatgcg cgcaagctgg
     8161 ccgcccgacc gatcatcatg gtgatcggcg acacagtctt cgcacatggc ggggtgctcc
     8221 ccgagcatgt ccgtcggggc atcgatgcga tcaatgagga gacgcagaag tggctccgcg
     8281 gcgaagggga gatctctccg gacatcctgc agggcgacga gagcccgctg tggacccgcc
     8341 gcttctgcca gagccccggc ccccgcgact gcgcggtcgc cagcgagacg ctgacgcagc
     8401 tcggcgtcaa gcgcatggtc gtcggccaca cggtccacaa ggacggcatc gtcaataact
     8461 gcgacggcaa ggtgtggtgc atcgacgtcg gcatggcggc gtactacggc ggccaccccg
     8521 aggtgctcga gatctccggc gatcaggtca agccgctgcg ctgagtcagg aggacagcac
     8581 cggcaagcgc cgcggacccg gcggctgcac gggcgcggcg gccggatcag ccttctcatg
     8641 gccacgagcg tgcaggcgag tgtcgagcag gtgggtcggg ctgacgttct tgagcgcgct
     8701 gagcatcacg gcgcgcacgt tcggcgagtc ctgctccatc tgcgtcagca gccgcgccat
     8761 cgcgtcgcga cgcaggccgt cctgcgagcc gcacaggttg cacggcaaga tcgggtaacc
     8821 gacgttgcgc gcgtgctcgg cgatgtcggc ctcggcacac tcgatcaacg ggcggatcac
     8881 ctggaagcgg ccgtcgtcgg tggtgtaacc ggccggcatc gcctgcagcc tgccgccgta
     8941 gaagagattt aataaaagag tctcgagggt gtcgtcgcgg tggtggccga gagcgatctt
     9001 gttgcagccg aggcgctcgg cggcggtata caaaattccc cggcgcagac gcgagcacac
     9061 ggcgcagtag gtgccgccgg gcggggtatg atcgaggacg accgagtagg tgtcctcgcg
     9121 caggatcttg tagggggcgc cgaagctctc gagccacgcg gccaatggac ggccgtcgta
     9181 gccgggctgg gcctggtcga ggtgcaccgc ggtgatgtcg aacttgaagg gcgcgcgggc
     9241 gcgggcgtgc cagagcaggt cgagcagcgt gtagctgtcc ttgccgccgg agacggcgac
     9301 gagcacgtgg tcgccctcgg ccagcaagtt gtagccggcg agcgtgcgcg tcatcgtctt
     9361 cgagagggcg cgacgcagcg ccccggggtc acgtgtactt tcggacatgt cgggctcagg
     9421 cgaggacggt gacggtgacg cggcggcggt ggccggcgtg gcggtgctcc cagagataaa
     9481 tgccctgcca ggtgccgagg tcgcagcggc cgtcgcggac gggcacggcg aggctggtct
     9541 gcgtcagcac cgtgcgcacg tgcgcgggca tgtcgtcggg gccctcgtcg tcatgtttaa
     9601 aaagggggtc gccgtcgggc acgaggcggg aaaggaagcg ctcgaggtcg cggtggacct
     9661 cggggtcggc gttctcggtg atcagcaacg aggcgctggt gtggtggatg aacaggtggc
     9721 acaggccggt cgcggcgccg ctgcgggcga cgatcgcctg gacctgggcg gtgatgtcga
     9781 ggatgccgcg gccgcgcgtg gcgacctcga tctgctcggc ggtcatcgct ggtggttggc
     9841 cttccactcg ctgtacggca tgccgtagac gcgctcgcgc gcctggtcgg tggtcagctc
     9901 gatgccctgc gccgtcgcgg cctcctgata ccagcgcgac aggcaattgc gacaaaagcc
     9961 cgccaggttc atcaggtcga ggttttgcac gtccttgcgg tcttgcaggt gcgcgaccag
    10021 gctgcgaaag gcggcggcct cgagctcgag ggcctgctgc ggggtcacgt tcgcgggggt
    10081 gttgtcctga gtcatgagcg tgccttttta aaattagaag aagatgccgt tgatcagggt
    10141 cagcggctcg cgggtcaggg tgcgccaggt gaagtcgagc agcacgcggt cgaagttgcc
    10201 gcgcgggtag gcggcgaaca cggccttgat ctcggcggag tcgaggccaa aacggaacag
    10261 accccggccg tgctcgatct ggacggcccg gcgaaatgcg tccgcctgcg ggctcaggtt
    10321 cggcaccggc atcaggcggt ggttgtagag catgcactcg tcgaggatct gcgccggcgt
    10381 gcccttgagg tcgtggccgg cggtctcgcg ctcgaacagc gcccggctgc gcgcgaggta
    10441 ggtcgggccg gtgtcgtcgc ggctgacgat gccgaggtcg tgacacatcg cgaccgtgta
    10501 cgcgacctcg cgg
//
LOCUS       DASWNJ010000179        13153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_568658, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000179 DASWNJ010000000
VERSION     DASWNJ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 13153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13153
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568658"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            518..1135
                     /gene="clpP"
                     /locus_tag="VGB85_03310"
     CDS             518..1135
                     /gene="clpP"
                     /locus_tag="VGB85_03310"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEY0133073.1"
                     /translation="MSPYANIPFVIEQTHRGERSWDIYSRLLKDRIIFLGTPVNDQVA
                     NVIIAQLLFLESEDPEKDITMYINSPGGVVTSGLAIYDTMQYVRPPVATYCLGQAASM
                     GSLLLAGGAPGKRFALPNARIMIHQPSGGAQGQATDIEIQAQEIKRLKSILNQILAEH
                     TSKTVDDILRDTERDNFMDAATAKSYGLIDEVLVRRKDGTPRVTS"
     gene            1132..2391
                     /gene="clpX"
                     /locus_tag="VGB85_03315"
     CDS             1132..2391
                     /gene="clpX"
                     /locus_tag="VGB85_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEY0133074.1"
                     /translation="MSSKRDNQGNLSCSFCGKSQKEVKKLIAGPSVYICDECISLCND
                     ILAEEVQKEERADVGAKVPKPKDIRKILDDYIVGQELAKKTISVAVYNHYKRINAKKL
                     PDEPELQKSNILLLGPTGSGKTLMAQTLARILDVPFAIADATSLTEAGYVGEDVENII
                     VNLLQNADHDVERAQRGIIYIDEIDKIARKGENPSITRDVSGEGVQQALLKILGGTVA
                     SVPPKGGRKHPQQDFLQVDTTNILFICGGAFAGLEDIIDHRMSAKTLGFGVQHAAKKE
                     LRQWELLKNVQSEDLMKFGLIPEFIGRLPIVAPLHDLDEAALVSILTQPKNALVKQYE
                     RLLGIDGVGLKFTEGALKAVAHKARAQKAGARGLRTILEKAMLDVMYEIPSSNNIREV
                     IVNEDTISQGKQPVVLYENEEAAKEAS"
     gene            complement(2441..2836)
                     /locus_tag="VGB85_03320"
     CDS             complement(2441..2836)
                     /locus_tag="VGB85_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133075.1"
                     /translation="MRPLVLWLVALVACEHQSDPATRYVPCPGGSTRCKKPCVAFGDS
                     SICGPTCDGLGRCGDESLAGNCLWDNQSPDEPGLCVVTCATDEDCPREGMECRQCTFS
                     EACAVGDALGAGPGVCAWPDGSYRGAALA"
     gene            complement(2842..5997)
                     /locus_tag="VGB85_03325"
     CDS             complement(2842..5997)
                     /locus_tag="VGB85_03325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5808 family serine peptidase"
                     /protein_id="HEY0133076.1"
                     /translation="MFGSLALALGLTTVQDTTAQAQKGEARAPGRVAQGYDATDLRIL
                     DWTLWYVAKYYVEPERIDPHKMAVAGLEGLEKAIPQVLVEPLAATPGQKAQRVRVRVG
                     TAEQEFQIGDVEALWAVGTHVREVFRFVNRHTTLSDEEQRAAEYSIVEGVLGTLDPHT
                     NLLRPDDFEQMKTSTKGSFGGLGIEVGMRDAQITVLRVIDGNPAAKAGIEAGDRIVQV
                     DEESTVTMGLSDAVDHMRGDPGTVVTLYIRREGSDKVKKVPITRDVITLDSVQGDILP
                     GTDAQGNPVKVGLVQINRNFAQNTGRELRAKLAEFEKAGVRGVVLDMRDNPGGLLTAA
                     VEVADAFLKTGTIVSTVGSSSPREELAADDRYDFPNLPVVVLIDQGSASATEIVAGAL
                     RNLGRAVLIGRRTFGKGSVQVLHDRKVADKELALKLTIAQYLTPGDVSIQSVGVSPDL
                     ETIPVLITKDYTAYHGRKRFDLVREESLASHLTSTKADPSHKIAAGPVYFLGNIYEEE
                     EEGEGKDKKAKKAATLKTKTDKSGRPTPEALLEDPEIRMARDLVVWAPTPSRDGILAK
                     IGDFVAQQTAIEQTRIVASLQKRGVDWAPGPAPEAGKTAVLRMAIRSDKAGNTIGAGE
                     SGTVTVTVTNEGTAPAFQVRAYSDSDYSYFDERELLFGRVDPGQTKSMPLKLSVSEHE
                     LSRTDRVEWQVFDQHGAKLAPGSQTTIDISSEGLPRPQFAFGYQILDDPKLGTNVRGN
                     ADGALQVGERVRMRVNFKNTGEGKAIDTWVTMRNLVGDAVFLHSGRENVKALAPGEAR
                     VVEFDLEVKKPAENGVVKLQLGVADAKIGASLAEKLSFPLAASTVTFAAGSGHVLAAS
                     GADLYGSPLKEQSQKVARAEPGSALKVLGVADGWYRVELEGKRFAFAKAADVKPGGSG
                     KPKVNDLMAISPPRIALVGAVTQTDGDRVHISGTATDDEAVRDVYISVYNPARNLFGS
                     SEKVFYQAASDPKTGRLEFAADIPLTPGNNIIEIRARENDDVAATKQMWVLRTSGLKE
                     ARAAQGKFTSNGKLSVDTLSK"
     gene            6304..7488
                     /locus_tag="VGB85_03330"
     CDS             6304..7488
                     /locus_tag="VGB85_03330"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HEY0133077.1"
                     /translation="MSERKRSPAGRPAGADKREKPVVDLVPTTPSRPVVAEVVDPTEV
                     VVAEPVDAEAVEAVDPEEAEESEAEEADEAELALAEVDPATDEELDAIAREIGRPSAA
                     VATTALARRDPMGQYMAEVRRYPLLTREEEHALAVRWIEHGDKEAGRRLVTSNLRLVV
                     KIAHEYKRTYQNLLDLVQEGNVGLIQAVKKFDPYRGVKLSTYSGWWIRAYILKFILNN
                     WRLVKIGTTQNQRKLFFNLRKQREKLVQAGVDPTAERIAKELDVSEAEVIEMDRRLSA
                     PDMSLNAPLGDDEGDGRTRLDIIEDSTDDPAMTVESAEFKEILQDKLRRFGATLAGRE
                     LEIFRDRIVADEPVTLQDLGDRWNVSRERARQLEKRMVLRLREFLQSELGDAVQIALG
                     QE"
     gene            7491..8354
                     /locus_tag="VGB85_03335"
     CDS             7491..8354
                     /locus_tag="VGB85_03335"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070677 - rRNA
                     (cytosine-2'-O-)-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEY0133078.1"
                     /translation="MEAEKKGLLVLVGTPLGNRGDLSPRAREAILGADLLLCEDTRSP
                     VRLLGEGVQLPPRISCFVVNEGERLGLLLEHLARGERVAYVSEAGLPVWSDPGLRLVQ
                     AAVAAGYEVDVVPGPTAAAVALCLSGLPSEDVRFLGFLPRSGPDRAARMRSIATDTAT
                     VLIYEAGNRTPALLRDLAAALPDAATRRVAIGRELTKQHQEVLRGTVQELASSLGDAL
                     RGEVTVVLGGAVASEVAAADPAQTAARAVWDLLWDTSLRPREKAKQLAALTGVDARAI
                     YERLSRDRAEE"
     gene            complement(8489..9955)
                     /locus_tag="VGB85_03340"
     CDS             complement(8489..9955)
                     /locus_tag="VGB85_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133079.1"
                     /translation="MRKLCGLLPLLGLYFGFSATTQAADVTRVASSFDGDNKFDLHFG
                     VGYDFNFKKAALLREWSDANNPSSTVVRDLLYQQFRHVVTPSMEIGLYKDLAVFVNVP
                     IVVSDNRTYSFDQEADPCRYGDNASLNEPATCVNHDNSTTIRDGIIPRGGFDATEPNN
                     PYNQFGGTDTKTIFKGATRRGVDQLHVGLKYALLNQSRLSHMPNWVIALEGRFAIGKP
                     MTFSRDTMGGNANNTSVGRGMHELGIWTSLSRRYRFLDPFFTAWWYQAIRANNTEFQK
                     FTDGAQDRVNPQSQTGVTFGTEIVPFERKAKGQKVAVVIRGLAMLKYNGRGYSEGWEL
                     FSDSPALIGSNAPGSGTPCTNQNARDAADWANDPANIGNADYIAGSGAPGSCSRFNGI
                     TNIQDFATFGINVGLNFQLSRYAMLMFGVDSRTDTRHWISNASRGKASLSGDPNLVEP
                     NTKEVNPVRRDVIDNVGRRYAVGDVLNVMGYFRFLLTF"
     gene            complement(10251..11780)
                     /locus_tag="VGB85_03345"
     CDS             complement(10251..11780)
                     /locus_tag="VGB85_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0133080.1"
                     /translation="MTQTTQHRILLVDDEPNLRKVLGALLRQQGYEVHAETDGAAALA
                     RVRSAPARTFDVVISDLRMPNLDGIGLLRALTVHEPNLPVILLTAHGSVDSAVEAVKL
                     GAFDYLEKPFDRDQIHQIVQKAIATRERSGPSATTAPPVEDLSDPCTHAGMVGRSPQM
                     QAIREIVATVAASPSTVLITAESGTGKELVARALHLGSPRHNQPFIKVNCAAIPAGLV
                     ESELFGHERGAFTGAVSSRAGRFELADGGTLFLDEVSEIPKEIQVKLLRAIQESEFER
                     VGGVKTLRVHVRLIAATNRSLEELIKEGQFREDLYYRLNVVPIHLPALRERIEDLPFL
                     VAHFVQRCNERLGKAVQGLTPAALTALERYPWPGNIRELENLVERMVLFASGPWVDED
                     DLPDAYAGEAHATTLPAESNTRLTLAEIAAAGGAGEGDDPRHIRLPLSSLGQDLKEAV
                     RAGSRLVEEALIREALAKTDGNVTRSARALGISRRSLQSKMKELGLRESPTEDPAKPA
                     G"
     gene            complement(11838..>13153)
                     /locus_tag="VGB85_03350"
     CDS             complement(11838..>13153)
                     /locus_tag="VGB85_03350"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0133081.1"
                     /translation="LVDPLRTELERRLEGWLFRERSALRAILVQLRTRLFNVIDPDEM
                     VKVVLDNLRESNRVTRAAFFLVDRQGLALTLRGHLGSPVVSRLDLAARRPLLERLRQG
                     PQLRDVLQRQRERADNELQAELDESLQTLAEVGASLALPVLGRATADDGRATYDLLGV
                     LFVEDERLLEPFSREEVELFTGVVAQAAVMIENSKVYEQRKERDRLAALGQMAAGLAH
                     EIRNPLGAIKGAVQVVEPAMQGADATTREFLGVIVEEVDRLNRVVTQFLSYSRPYSGE
                     MSAIQLGDVLTAALRLLGEDNRQRVTVTIAPNLPRIRGDAEALRQVLLNLLRNAFDAI
                     NGQSSPGRVWIHVGLRRHGLASGDAVTLSVRDNGPGFTPSALANLFVPFHTTKSGGTG
                     LGLPISQRIVENHRGLIEASAPEGGGAQFTVVLPIEPLPRAAAPV"
BASE COUNT         2000 a         4315 c         4811 g         2027 t
ORIGIN      
        1 ctcgacttcc tgatgggcaa ggccgaggtc accgaggcgc cggatccggc gcccgaaacc
       61 ggtagcgacg cgaccgggag cgcctcgctc ggcgagtccg gcggcggcac cggcgagact
      121 gccaccgcga cctgagcgcg acccgggcgc gacccggcgc accggcgtcg aaccacagcg
      181 cagcgcgccg gcacacccgc cggacgctgc gctgaagttt ttggccgtcc cgatcacgct
      241 cgacgggtcc ggcgccgccc gccgcgttcg cggcgcgcag cctcgcatgc gctcgtatcg
      301 agcctcgcaa gcgacggcgg tacgcgcgac agatcgcagg ccgagcgtgc tatcaccgtc
      361 cggtcgccat tgcgagcgga cgcgcgtccg catgcaccac cgagccggtt tgtcatcggc
      421 cttctgtcat tgttcgtccg accggcgacc tcgccacctc cacccccttg catcgtcggg
      481 cgcgcccagt caccatagcc aacgggacga ccaccacgtg agcccctacg ccaacattcc
      541 attcgtcatc gagcagacgc atcgcggcga gcgctcgtgg gacatctaca gccggctcct
      601 gaaggaccgc atcatctttc ttggcacgcc ggtcaacgac caggtcgcca acgtgatcat
      661 cgcccagctg ctcttcctcg agtctgaaga cccggagaag gacatcacga tgtacatcaa
      721 ctcgcccggc ggcgtggtga cctcgggcct ggcgatctac gacacgatgc aatacgtgcg
      781 cccgccggtc gccacgtact gcctcggcca ggcggcgtcg atgggctcgc tgctgctcgc
      841 gggcggcgct cctggtaagc ggttcgcgct gccgaacgcg cgcatcatga tccatcagcc
      901 ctcgggtggt gcgcaggggc aggccaccga catcgagatc caggcgcagg agatcaagcg
      961 cctgaagtcg atcctcaacc agatcctggc tgagcacacc agcaagaccg tcgacgacat
     1021 cctgcgcgac accgagcggg acaacttcat ggacgcggcg accgccaagt cctacggtct
     1081 catcgacgaa gtcctggtcc gccgcaagga cgggactcca cgggtgacct catgagctcc
     1141 aagcgcgaca atcaaggcaa cctcagctgc tccttttgcg gcaagagcca aaaggaggtc
     1201 aagaagctca tcgcagggcc ttcggtctac atctgcgacg agtgcatctc cctctgcaac
     1261 gacatcctcg ccgaggaggt ccagaaggag gagcgtgccg atgtcggcgc caaggtcccc
     1321 aagcccaagg acatccgcaa gatcctcgac gactacatcg tcggccagga gctcgccaag
     1381 aagaccatct ccgtagcggt ctacaaccac tacaagcgca tcaacgccaa gaagctcccg
     1441 gacgagcccg agctgcagaa gagcaacatc ttgctgctcg gcccgaccgg ctccggcaag
     1501 acgctgatgg cgcagacgct cgcgcgcatc ctcgacgtgc ccttcgcgat cgcggacgcg
     1561 accagcctga ccgaggccgg ctacgtcggc gaggacgtcg agaacatcat cgtcaacctg
     1621 ctgcagaacg ccgaccacga cgtcgagcgc gcgcagcggg gcatcatcta catcgacgag
     1681 atcgacaaga tcgcccgcaa gggtgaaaac ccgtcgatca cccgcgatgt ctccggcgag
     1741 ggcgtgcagc aggcgctgct caagatcctc gggggcacgg tcgccagcgt gccgcccaag
     1801 ggcggtcgca agcacccgca gcaggacttc ttgcaggtcg acacgaccaa catcctgttc
     1861 atctgcggcg gcgccttcgc gggcctcgag gacatcatcg accaccgcat gtcggcgaag
     1921 acgctcggct tcggggtcca gcacgcggcc aagaaggagc tccgccagtg ggagctgctc
     1981 aagaacgtgc agagcgagga cctgatgaag ttcgggctga tccccgagtt catcggccgc
     2041 ctgccgatcg tcgccccgct gcacgacctc gacgaggccg cgctggtgtc gatccttacg
     2101 cagccgaaga acgcgctggt gaagcagtac gagcgcctgc tcggcatcga cggcgtcggc
     2161 ctcaagttca ccgagggcgc gctcaaggcc gtcgcccaca aggcccgcgc gcaaaaggcc
     2221 ggcgcccgcg gcctccgcac gatcctcgag aaggcgatgc tcgacgtgat gtacgagatc
     2281 ccgagctcga acaacatccg cgaggtcatc gtcaacgagg acaccatctc gcagggcaag
     2341 cagcccgtgg tcctctacga gaacgaggaa gccgccaaag aggcgagctg agccacagcg
     2401 agcgaagaag gagggtcggc cagcgccggc ccttcttcgt ttaagcgagc gcagcgccgc
     2461 gataagagcc atcgggccac gcgcagacgc ccgggcctgc accgagggca tcgccgacgg
     2521 cgcaggcttc ggagaaagtg cactggcggc actccatccc ttcgcgcggg caatcctcgt
     2581 cggtcgcaca ggtgaccacg cacaggcccg gctcgtccgg gctctgattg tcccacaggc
     2641 aattgcccgc cagcgactcg tcgccgcagc ggcccagacc gtcgcaggtc gggccgcata
     2701 tcgacgaatc gccgaacgcc acgcagggct tcttgcagcg cgtgctgccc ccggggcagg
     2761 ggacgtagcg tgtggcgggg tcgctctggt gctcgcaggc gacgagcgcg acgagccaca
     2821 gaacgagcgg ccgcatcacc ttcacttgct cagcgtgtcg acgctgagct tgccgttgct
     2881 ggtgaacttg ccctgcgcgg cgcgggcctc cttgaggccg ctggtgcgca gcacccacat
     2941 ctgcttggtc gcggcgacgt cgtcgttctc gcgggcgcgg atctcgatga tgttgttgcc
     3001 gggggtcagc gggatgtcgg cggcgaactc gaggcggccg gtcttcgggt cggaggcggc
     3061 ctgatagaac accttctccg agctgccgaa caggttgcgc gcggggttgt agacgctgat
     3121 gtacacgtcg cgcacggcct cgtcgtcggt cgcggtgccg ctgatgtgca cgcggtcgcc
     3181 gtcggtctgg gtgaccgcgc cgacgagcgc gatcctcggc ggcgagatcg ccatcaggtc
     3241 gttgaccttc ggcttgccgc tgccaccggg cttgacgtcc gccgccttgg cgaacgcgaa
     3301 gcgcttgccc tcgagctcga cgcgatacca gccgtcggcg acgccgagca ccttgagcgc
     3361 gctgccgggc tcggcgcggg cgaccttttg cgactgctcc ttgagcgggc tgccgtacag
     3421 gtcggcgccg ctggcggcga gcacgtggcc cgagccggcc gcgaaggtga cggtgctcgc
     3481 cgcgagcggg aagctgagct tctccgccag cgacgcaccg atcttcgcgt cggcgacgcc
     3541 gagctggagc ttgaccacgc cgttctcggc gggcttcttc acctcgaggt cgaactcgac
     3601 cacccgcgcc tcgccgggcg cgagcgcctt gacgttctcg cggccgctgt gcaggaacac
     3661 cgcgtcgccg acgaggttgc gcatcgtcac ccaggtgtcg atcgccttgc cctcgccggt
     3721 gttcttgaaa ttgacgcgca tgcgtacgcg ctcgccgacc tgcagggcgc cgtcggcgtt
     3781 gccgcggacg ttggtgccga gcttcgggtc gtcgaggatc tggtagccga acgcgaactg
     3841 cgggcgcggc aggccctcgc tgctgatgtc gatcgtcgtc tgcgagcccg gcgcgagctt
     3901 ggcaccgtgc tggtcgaaca cctgccactc gacgcggtcc gtgcgcgaca gctcgtgctc
     3961 cgagaccgag agcttgagcg gcatgctctt ggtctggccg gggtcgacgc ggccgaacag
     4021 cagctcgcgc tcgtcgaagt agctgtagtc ggagtcggag taggcgcgca cctggaaggc
     4081 cggggcggtg ccctcgttgg tgacggtgac ggtgacggtg cccgactcgc cggcgccgat
     4141 cgtgttgccg gccttgtcgc tgcggatcgc catgcgcagc acggcggtct tgccggcctc
     4201 cggcgcgggg ccgggggccc agtcgacgcc gcgcttttgc agcgaggcga cgatgcgggt
     4261 ctgctcgatc gccgtctgct gggcgacgaa gtcgccgatc ttggcgagga tgccgtcgcg
     4321 gctcggggtc ggggcccaca cgacgaggtc gcgggccatg cggatctccg ggtcctcgag
     4381 cagcgcctcc ggggtcgggc ggccggactt gtcggtcttg gtcttgaggg tggcggcctt
     4441 cttcgccttc ttgtccttgc cctcgccctc ctcctcctct tcgtaaatat tgccgaggaa
     4501 gtacaccggg ccggcggcga tcttgtgcga ggggtcggcc ttggtcgagg tcaggtgcga
     4561 ggcgagcgac tcctcgcgca cgaggtcgaa gcgcttgcgg ccgtgatagg cggtgtaatc
     4621 cttggtgatc aggacgggga tcgtctcgag gtcggggctg acgccgaccg actggatcga
     4681 tacgtccccc ggcgtcaggt attgggcgat ggtcagcttg agcgcgagct ccttgtcggc
     4741 gaccttgcgg tcgtgcagga cctgcaccga gcccttgccg aaggtgcggc ggccgatcag
     4801 caccgcgcgg ccgaggttgc gcagcgcgcc ggcgacgatc tccgtcgcgc tcgccgagcc
     4861 ctggtcgatg agcacgacga cgggcaggtt cgggaagtcg tagcggtcgt cggcggcgag
     4921 ctcctcgcgc ggcgaggacg agccgacggt cgagacgatc gtgccggtct tgaggaaggc
     4981 gtcggcgacc tcgacggcgg cggtcagcag gccgccgggg ttgtcgcgca tgtcgagcac
     5041 gacgccgcga acgccggcct tctcgaactc ggcgagcttg gcccgcagct ccctgccggt
     5101 gttctgggcg aagttgcggt tgatctggac caggccgacc ttgaccgggt tgccctgcgc
     5161 gtcggtgccg gggaggatgt cgccctgcac gctgtcgagg gtgatcacgt cgcgcgtgat
     5221 cgggaccttc ttgaccttgt cgctgccctc gcggcgaatg tagagcgtga cgacggtgcc
     5281 ggggtcgccg cgcatgtggt cgacggcgtc gctcaggccc atcgtgacgg tcgactcctc
     5341 gtcgacctgg acgatgcggt cgccggcctc gatgccggcc ttggcggcgg ggttgccgtc
     5401 gatcacgcgc agcacggtga tctgcgcgtc gcgcatgccg acctcgatgc cgaggccgcc
     5461 gaagctgccc ttggtgctgg tcttcatctg ctcgaagtcg tcggggcgca gcaggttggt
     5521 gtgcgggtcg agcgtgccga gcacgccctc gacgatcgag tactccgccg cgcgctgctc
     5581 ctcgtccgag agggtggtgt ggcggttgac gaagcggaac acctcgcgca cgtgggtgcc
     5641 gacggcccac agcgcctcga cgtcgccgat ctggaattcc tgctccgcgg tgccgacgcg
     5701 cacgcgcacg cgctgggcct tttgtcccgg ggtcgcggcg agcggctcga cgagcacctg
     5761 cgggatcgcc ttctcgaggc cctcgaggcc ggcgacggcc atcttgtgcg ggtcgatgcg
     5821 ctcgggctcg acgtagtact tggcgacgta ccacagggtc cagtcgagga tcctcaggtc
     5881 ggtcgcgtcg tagccctgcg cgacgcgacc aggggcgcgt gcctcgccct tctgggcctg
     5941 cgccgtggtg tcctgcacgg tcgtcaggcc gagcgcgagg gcgagtgatc cgaacacgat
     6001 ggccggcgtg gaggccttgg caaaacgggg gcgagacggt gtttgtccgg tcacaaactc
     6061 agtctaacgg cctgcgcagg gcgaggccac cacacgcgcg agtgattgtc gggaagcacc
     6121 ggcgtgaaac catgctcgag cactgcgggt cacgggaggt tcctgacagg tcgctggcgg
     6181 gtcgaccgcg cgtgttcgcg ggcgacgcgc aggaaagtcg cccgaagtcg caaacccgct
     6241 cgcacgttct cggggcagac ccgggtaccc aagtggggcg ctcgcggcgt agactccgcc
     6301 agcatgtcgg aacggaagcg cagtccagcg gggcggcccg ctggcgcgga caagcgagag
     6361 aagcccgtcg tcgacctcgt gccgaccacc ccgagtcggc cggtggtggc ggaggtggtc
     6421 gacccaacgg aggtcgtggt tgcggagccc gtcgacgcgg aggcggtcga ggcggtcgac
     6481 cccgaggagg ccgaggagtc cgaggccgag gaggcggacg aggccgagct cgcgctcgcc
     6541 gaggtcgacc cggcgacgga cgaggagctc gacgcgatcg cccgtgagat cggtcggccg
     6601 agtgccgcgg tcgcgaccac tgcgctggcc cgacgcgacc cgatgggcca gtacatggcc
     6661 gaggtccgcc gctaccccct gctgacccgc gaggaggagc acgcgctcgc ggtgcggtgg
     6721 atcgagcacg gcgacaagga ggcgggccgt cggctggtga cctccaacct gcggctcgtg
     6781 gtgaagatcg cccacgagta caagcgcacc tatcaaaacc tcctcgatct ggtccaggag
     6841 ggcaacgtcg ggctgatcca ggcggtcaag aagttcgacc cgtaccgcgg ggtcaagctc
     6901 tcgacctact ccggctggtg gatccgggcg tacatcctca agttcattct caataactgg
     6961 cggctggtga agatcggcac gacccagaac cagcgcaagc tgttcttcaa cctgcgaaag
     7021 cagcgcgaga agctcgtgca ggccggcgtc gacccgaccg ccgagcgcat cgccaaggag
     7081 ctcgacgtca gcgaggccga ggtcatcgag atggaccgcc gcctgtcggc gccggacatg
     7141 tcgctcaacg ccccgctcgg cgacgacgag ggcgacggcc gcacgcggct cgacatcatc
     7201 gaggactcga ccgacgaccc ggcgatgacg gtcgagtcgg cggagttcaa ggagatcctg
     7261 caagacaagc tgcgccgctt cggcgcgacg ctggccggcc gcgagctcga gatctttcgt
     7321 gaccggatcg tcgccgacga gccggtgacg ctgcaggacc tcggcgaccg ctggaacgtc
     7381 agccgcgagc gggcgcgcca gctggagaag cgcatggtgc tgcgcctgcg cgagttcctg
     7441 cagagcgagc tcggcgacgc cgtgcagatc gctctgggcc aggagtgagc gtggaggccg
     7501 agaagaaggg cctgctggtg ctcgtcggca cgccgctggg caaccgcggc gacctctcgc
     7561 cgcgcgcgcg cgaggcgatc ctcggcgccg acctgctgtt gtgcgaggac acgcgcagcc
     7621 ccgtgcggct gctcggcgag ggcgtacagc tgccaccgag gatcagctgc ttcgtcgtca
     7681 acgagggcga gcgcctcggg ctcctgctcg agcacctcgc acgtggcgag cgcgtcgcgt
     7741 acgtctcgga ggccggcctg ccggtgtgga gcgacccggg cctgcggctg gtccaggcgg
     7801 cggtcgcggc gggatacgag gtcgacgtcg tgccggggcc gaccgccgcg gcggtcgccc
     7861 tgtgcctcag cgggctgccc tccgaggatg ttcgcttcct cgggttcttg ccgcgctcgg
     7921 ggcccgaccg ggcggcgcgc atgcggtcga tcgcgaccga cacggcgacc gtgctgatct
     7981 acgaggccgg caaccggacg ccggcgctgc tgcgcgacct cgcggcggca ctgccggacg
     8041 cggcgacccg gcgcgtggcg atcgggcgcg agctgaccaa gcagcatcag gaggtcctgc
     8101 gcggcacggt gcaagagctg gcgagcagcc tcggcgacgc gctgcgcggc gaggtgacgg
     8161 tcgtgctcgg cggcgcggtc gccagcgagg tcgcggcggc cgacccggcc cagacggccg
     8221 cacgtgcggt gtgggacctg ctgtgggaca cgagcttgcg cccgcgcgag aaggccaagc
     8281 agctcgccgc gctgaccggc gtcgacgcgc gggcgatcta cgagcggctc tcgcgtgacc
     8341 gcgcagagga gtgaggcgcg ccggaccggc ttcgacgcgg agtgggccga gcagctcacg
     8401 gtgccgacac ggcgactccc gcgagtgaga gtccttaaat gcgaacgccg cccggtgggc
     8461 ggcgtgcgtc gagggcctgc tggaccgttt agaaggtcag caggaagcgg aagtagccca
     8521 tcacgttgag cacgtcgccg acggcgtagc ggcggccgac gttgtcgatg acatcgcggc
     8581 ggaccgggtt gacctctttg gtgttcggct cgacgaggtt ggggtcaccg ctgagggacg
     8641 ccttgccgcg gctggcgttg gagatccagt ggcgggtgtc ggtgcggctg tcgacgccga
     8701 acatcagcat cgcgtagcgg ctgagctgga agttgagacc gacgttgatg ccgaaggtcg
     8761 caaagtcctg gatgttcgtg atgccgttga agcgcgagca gctgccgggg gcgccggagc
     8821 cggcgatgta gtcggcgttg ccgatgttgg cggggtcgtt ggcccagtcg gcggcgtcac
     8881 gcgcgttctg gttggtgcac ggggtacccg agcccggcgc gttgctgccg atcagcgcgg
     8941 ggctgtcgga gaacagctcc cagccctcgc tgtagccgcg gccgttgtac ttgagcatcg
     9001 ccaggccgcg gatgacgacg gcgacctttt gacccttggc cttgcgctcg aaggggacga
     9061 tctcggtgcc gaaggtgacg ccggtctggg actgcgggtt gacccggtcc tgcgcgccgt
     9121 ccgtgaactt ctggaactcg gtgttgttgg cgcggatcgc ctggtaccac caggcggtga
     9181 agaacgggtc gaggaagcgg tagcggcgcg agagcgaggt ccagatgccc agctcgtgca
     9241 tgccgcgacc gaccgacgtg ttgttcgcgt tgccacccat cgtgtcgcgc gagaaggtca
     9301 tcggcttgcc gatggcgaac cggccctcga gggcgatgac ccagttcggc atgtgcgaca
     9361 ggcggctctg gttgagcagc gcgtacttga ggccgacgtg cagctggtcg acgccacgac
     9421 gcgtcgcgcc cttgaagatc gtcttcgtgt ccgtgccccc gaactggttg tacgggttgt
     9481 tcggctccgt ggcatcgaag ccgccgcgcg ggatgatgcc gtcgcggatc gtcgtgctgt
     9541 tgtcgtggtt gacgcaggtc gccggctcgt tgagcgacgc gttgtcgccg tagcgacacg
     9601 ggtcggcttc ctggtcgaag ctgtaggtcc ggttgtccga gaccacgatc ggcacgttca
     9661 cgaagaccgc gaggtccttg tagagaccga tctccatcga cggcgtcacg acgtggcgaa
     9721 actgctggta cagcaggtcg cgcacgacag tgctgctcgg gttgttggcg tcgctccact
     9781 cgcgcaacaa cgcggccttc ttgaagttga agtcgtagcc aaccccgaag tgcaggtcga
     9841 acttgttgtc gccgtcgaac gagctcgcca cgcgagtgac gtccgctgcc tgcgtcgtcg
     9901 cgctgaatcc gaagtacagg cccagtaggg gcaggaggcc gcacagtttc cgcataggga
     9961 gcacctcagg gagtcggtgg acgatacctg cgcgggcttg ccagcgtcaa cattcctggg
    10021 tccgggaagt cccagcagac ccgcgccggg gcaggcgaat tcgcgtgcct tggcgccctc
    10081 cacggcggtc taggcggtga ttgacgggcg atctgcgccg aaactttcga ggagatcgcg
    10141 gccgcgggcg gcccccacgg agggccggat cccaaaaaaa ccggggccgg ccgggggtcg
    10201 aggccggccc cggccggtga aaaccaggtc ggccggcgcc gcggggcggc tcagccggcg
    10261 ggcttcgccg ggtcctcggt cgggctctcg cgcaggccga gctccttcat cttcgactgg
    10321 aggctgcggc ggctgatgcc gagggcgcgg gccgagcggg tgacgttgcc gtcggtcttg
    10381 gccagggcct cgcggatcag ggcctcctcg accaggcgag agccggcgcg caccgcctcc
    10441 ttgaggtcct ggccgaggct cgaaagcggc aagcggatgt gccgcgggtc gtcgccctcg
    10501 ccggcgccgc cggccgcggc gatctccgcg agcgtcagcc gggtgttcga ctcggcaggt
    10561 aaggtggtcg cgtgggcctc cccggcgtag gcgtcgggga gatcgtcctc gtcgacccag
    10621 ggccccgagg cgaacaggac catgcgctcg acgaggttct cgagctcgcg gatgttgccc
    10681 ggccacgggt agcgctcgag cgcggtcagg gcggcggggg tcaggccctg cacggccttg
    10741 ccgagccgct cgttgcagcg ctgcacgaag tgggcgacca ggaacggaag gtcctcgatg
    10801 cgctcgcgca gcgcgggcag gtggatcggg acgacgttga gccggtagta gaggtcctcg
    10861 cggaactggc cctccttgat cagctcctcg aggctacggt tggtcgcggc gatcaggcgt
    10921 acgtgtacgc gcaaggtctt gacgccgccg acgcgctcga attccgactc ttgaatagcc
    10981 cgcagcagct tgacctggat ctccttgggg atctcgctga cctcgtcgag gaacaaagtg
    11041 ccgccgtcgg cgagctcgaa gcggccggcc cggctcgaga cggccccggt gaaggcgccg
    11101 cgctcgtgac caaacagctc actttcgacc aggccggccg ggatcgcggc gcagttgacc
    11161 ttgatgaagg gctggttgtg ccgcggcgag ccgaggtgca gggcccgcgc gaccagctcc
    11221 ttgccggtgc cgctctcggc ggtgataagg acggtcgagg ggctggcggc gacggtcgct
    11281 acgatctcgc ggatcgcctg catctgcggc gagcgcccga ccatgccggc gtgggtgcag
    11341 ggatcgctca ggtcctcgac cggcggggcg gtggtcgccg acgggccgga ccgctcgcgc
    11401 gtggcgatcg ccttctggac gatctgatgg atctggtcgc ggtcgaaggg cttctccagg
    11461 tagtcgaagg cgccgagctt cacggcctcg accgccgagt cgacgctgcc gtgcgccgtc
    11521 agcaagatca ccgggaggtt gggctcgtgc acggtcagcg cgcgcagcag accgatgccg
    11581 tcgaggttgg gcatccgcag gtcggagatc acgacgtcga aggtgcgcgc cggcgccgag
    11641 cggacccggg ccagcgccgc cgccccgtcc gtctccgcgt ggacctcgta gccctgctgc
    11701 cgcagcagcg caccgaggac ctttcgcagg ttgggttcgt cgtcgaccag gaggatgcgg
    11761 tgctgtgtcg tctgggtcac ggtggcttcg gatggcgaaa gggacatgtg cgcgaggaca
    11821 ggtgcgaaag acggggctca aacgggggca gcggctcgcg gcagcggctc gatcgggagg
    11881 acgacggtga actgggcgcc gccgccctcg ggggcgctgg cctcgatcag gccgcggtga
    11941 ttctccacga tccgctgcga gatcgggagg cccaggccgg tgccgccgct cttggtggtg
    12001 tggaacggga cgaacaggtt ggcgagcgcc gacggggtga agccggggcc gttgtcgcgg
    12061 acgctcagcg tgacggcgtc gccgctggcg agcccgtggc ggcgcaggcc gacgtggatc
    12121 cacacgcggc cgggggagct ttgcccgttg atcgcgtcga aggcgttgcg aaggaggttg
    12181 agcaggacct gacgcagggc ctcggcgtcg ccgcggatcc ggggcaagtt cggcgcgatc
    12241 gtgacggtga cgcgctggcg gttgtcctcg ccgagcaggc gcagcgcggc ggtcagcacg
    12301 tcgccgagct ggatcgccga catctcgccc gagtaggggc gcgagtagct gaggaactgg
    12361 gtgacgacgc ggttgaggcg gtcgacctcc tcgacgatga cgccgaggaa ctcgcgcgtg
    12421 gtcgcgtcgg cgccttgcat ggccggctcg acgacctgca cggcgccctt gatcgcgccg
    12481 agggggttgc ggatctcgtg ggcgaggccg gcggccatct ggccgagcgc ggcgaggcgg
    12541 tcgcgctcct tgcgttgctc gtacaccttc gagttctcga tcatcaccgc agcttgggcg
    12601 acgacgccgg tgaacagctc gacctcctcg cgcgagaagg gctcgagcag gcgctcgtcc
    12661 tcgacgaaga ggacgccgag caggtcgtag gtggcgcggc cgtcgtcggc ggtggcgcgg
    12721 ccgaggaccg gcagcgcgag gctggcaccg acctcggcga gggtctgcag ggactcgtcg
    12781 agctcggctt gcagctcgtt gtcggcgcgc tcgcgctggc gctggaggac gtcgcgcagc
    12841 tgcgggccct ggcgcaggcg ctcgagcagg ggtcgacggg cggcgaggtc gaggcgggat
    12901 acgacggggc tgccgaggtg gccgcgcagg gtgagtgcga ggccctggcg gtcgacgagg
    12961 aagaaggcgg cccgggtgac gcggttggac tcgcgcaggt tgtcgagcac gaccttgacc
    13021 atctcgtcgg ggtcgatgac gttgaacagg cgggtgcgca gctggacgag gatggcgcgc
    13081 agggcggagc gctcgcgaaa cagccagccc tcgagtcggc gctcgagctc ggtgcgcagc
    13141 gggtcgacga gca
//
LOCUS       DASWNJ010000180         2636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_356068, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000180 DASWNJ010000000
VERSION     DASWNJ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356068"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VGB85_03355"
     CDS             <1..522
                     /locus_tag="VGB85_03355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0133082.1"
                     /translation="YHLFRVDEPTTGALAGYATIAVDMSRRMRAADDHRFLADSVPQL
                     LSTLNPAGDITYTNRRTREFTGRTHEELSLNWTDLIHPDDLERTLAAWTAAQVDGRAY
                     QLEHRLRRRDGEYRWVLLHAVPQHDADGRVLQWVRASTDIHAIHLIKERSADLEAATR
                     RIRELEAQLAARG"
     gene            complement(519..1934)
                     /locus_tag="VGB85_03360"
     CDS             complement(519..1934)
                     /locus_tag="VGB85_03360"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0133083.1"
                     /translation="MTTSPRADRLADHLANLRERVQAALGPAVLVPDDGRYAHDDGAE
                     GPFPPGLVVRPGTQAEVVELVRLCHALAVPLSPRGAGTGTVGGCLADHGGVVLDLAGL
                     DRILDIDERSLLAEVEPGVITATLHASVEARGLFYPPDPASLKKCSLGGNVATNAGGP
                     RACKYGVTGNFVLGLDVVTGTGQALRVGHRSIKGVTGYNLAGLLVGSEGTLAVTTACT
                     LRLVPLPACAVTLLASFADEADACAAVHRCQTGGVTPTALEFADRLCVQVLLAGGELA
                     VPVPAEARALLWIEIDGDTAGLDASIRRAAQHCREAGALAVTVAEDSVTRERLWDVRR
                     ALSHTLRARWPKKISEDVAVPLAQLPRLAEAAREIGAAQGLDVASFGHAGDGNLHVNI
                     LYDPQASADVMTRVDRAVHDLFRVTIALGGTLSGEHGIGISKRPFMPLEQSEPLRALQ
                     IELKRAWDPGNILNPGKLFPA"
     gene            complement(1931..2356)
                     /gene="erpA"
                     /locus_tag="VGB85_03365"
     CDS             complement(1931..2356)
                     /gene="erpA"
                     /locus_tag="VGB85_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEY0133084.1"
                     /translation="MSATHEHTHEHPTTAQPSSGLAPMPAQDDPEMAGGLGVTETAAA
                     KIREIREAEAIEGSYALRVKVMGGGCSGFQYDLYFDEPAEGDQAFTSNGVSLICDQMS
                     FMYLMGTQIDYVEGLQGAGFKFGNPNTTGSCGCGSSFSV"
BASE COUNT          375 a          925 c          967 g          369 t
ORIGIN      
        1 tatcacctgt ttcgcgtcga cgagccgacc accggcgcgc tcgcgggcta cgcgacgatc
       61 gcggtcgaca tgtcgcggcg catgcgtgcc gcggacgacc accgcttcct cgccgactcg
      121 gtgccgcagc tgctctcgac cctcaacccc gcgggcgaca tcacgtacac gaaccgccgc
      181 acgcgcgagt tcaccggccg cacccacgag gagctgagcc tcaactggac cgacctgatc
      241 caccccgacg acctcgaacg cacgctcgcc gcctggaccg ccgcgcaggt cgacgggcgc
      301 gcctaccagc tcgagcatcg gctgcgccgc cgcgacggtg agtaccgctg ggtgctgctg
      361 cacgccgtcc cgcagcacga cgccgacggc cgcgtgttgc agtgggtccg cgcgagcacc
      421 gacatccacg cgatccactt gatcaaggag cgcagcgccg acctcgaggc tgcgacgcgg
      481 cggatccgcg agctcgaggc gcagctggcc gcgagaggct aggccgggaa cagcttgccc
      541 gggttgagga tgttgcccgg gtcccacgcg cgcttgagtt ctatctgcag cgcccgcagc
      601 ggctccgact gctcgagcgg catgaacggt cgcttgctga tgccgatccc gtgctccccg
      661 ctgagcgtgc cgccgagcgc gatcgtcacg cggaacaggt cgtgcaccgc ccggtcgacg
      721 cgcgtcatga cgtccgcgct cgcctgcggg tcataaagaa tgttgacgtg caggttgccg
      781 tcgccggcgt ggccgaagct ggcgacgtcg aggccctggg ccgcaccgat ctcgcgggcg
      841 gcctcggcga ggcgcggtag ttgggccagc gggaccgcga cgtcctcgct gatcttcttc
      901 ggccagcggg cgcgcagcgt gtggctgagc gcgcgacgga cgtcccacag gcgctcgcgt
      961 gtgacgctgt cctcggcgac cgtgacggcg agcgcaccgg cctcgcggca gtgctgcgcg
     1021 gcgcgacgaa tgctggcgtc gaggccggcc gtatcgccgt cgatctcgat ccacagcagg
     1081 gcgcgcgcct ccgcgggcac cggcaccgcg agctcaccgc cggcgagcag gacctgcacg
     1141 cacaggcggt cggcaaactc gagcgcggtc ggggtgacgc ctcccgtctg acagcgatgc
     1201 acggcggcgc aggcgtcggc ctcgtcggcg aagctggcca gcaaggtcac cgcgcaggcc
     1261 ggcagcggga ccaggcgcag ggtgcaggcg gtggtgaccg cgagcgtgcc ctcggagccg
     1321 acgagcagcc ccgcgaggtt gtacccggtg acgcctttga tcgaccgatg gccgacgcgc
     1381 agcgcttgcc cggtgccggt gacgacgtcg aggccgagca cgaagttgcc ggtgacgccg
     1441 tacttgcacg cccgcgggcc gcccgcgttg gtcgcgacgt tgccgccgag gctgcacttt
     1501 tttaaagaag ccgggtccgg cgggtagaac aggccgcgag cctcgaccga agcatgcagc
     1561 gtcgcggtga tgacgcccgg ctcgacctct gccagcaacg agcgctcgtc gatgtcgagg
     1621 atgcggtcga ggccggcgag gtcgagcacg acgccgccgt ggtccgcgag gcagccgccg
     1681 accgtgccgg tgccggcgcc gcgcgggctc agcggcaccg cgagcgcgtg gcacagccgg
     1741 accagctcga cgacctccgc ctgcgtgccg ggccgaacga ccaggccggg cgggaagggg
     1801 ccctcggcgc cgtcgtcgtg ggcgtagcgg ccgtcgtcag ggacgagcac cgcgggtccg
     1861 agcgcggcct ggacccgctc gcgcaaattg gcgaggtggt cggcgagtcg gtccgcgcga
     1921 gggctcgtgg tcacaccgag aacgagctgc cgcagccgca gctgccggtg gtgttcgggt
     1981 tgccgaactt gaagcccgcc ccctgaaggc cctcgacgta gtcgatctgg gtgcccatga
     2041 ggtacatgaa gctcatctgg tcgcagatga gcgacacgcc gttgctggtg aacgcctggt
     2101 cgccctcggc gggctcgtcg aagtacaggt cgtactggaa gccgctgcag ccgccgccca
     2161 tgaccttgac gcgcagggcg tagctgccct cgatcgcctc ggcttcgcgg atctcgcgga
     2221 tcttcgcggc ggcggtctcg gtcaccccga ggcccccggc catctccggg tcgtcctgcg
     2281 caggcatggg ggccaaaccc gaggagggct gcgcggtcgt cgggtgctcg tgggtgtgtt
     2341 cgtgggtcgc ggacatggca gctcctgcaa gagcctcggc tctcgacgtc cgggtaagat
     2401 gcgtgcgggc ccgggtccgg tcaagccggg ggatcggcgc ggaacgtgag gcaaagccga
     2461 agcgcgggcg tttggcgcgg gtggtgcgga cccgcgccca ccgccggcgg caccggagcg
     2521 ccatcgttcg cccgtggcgc ggtttgacag aggcgcggcg cttcccatac cgtgacggcg
     2581 agccatttgc cgcagatttg cccggcatag gctcggagaa cgcatgcgac tgtcct
//
LOCUS       DASWNJ010000181         1692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_74612, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000181 DASWNJ010000000
VERSION     DASWNJ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74612"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VGB85_03370"
     CDS             <1..239
                     /locus_tag="VGB85_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133085.1"
                     /translation="SDHVIMSGPPLKKFRYEYPASEARFLAAPTAEAAVRYIRRAYPH
                     NALDVLPTLRELARWPEFWRTVDDQGLLLPREPT"
     gene            complement(275..1111)
                     /locus_tag="VGB85_03375"
     CDS             complement(275..1111)
                     /locus_tag="VGB85_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HEY0133086.1"
                     /translation="MSAALGIGFSPCPNDTFMFHGLVHGVVPGLVVAPWLADIEALNV
                     RAIAGRDPLPITKLSVHALAHVSDRYTVLSAGAALGRGCGPLLVTRPELAGGAAGIGQ
                     MRVAIPGRYTTALLLLRALLPAPRELVSMTFSEILPAVASGSVDAGLIIHESRFTYAD
                     HGLVQLADLGLLWEAETGLPLPLGVIVAERAQGEALLERVEDGIRRSLAAAWERPEAP
                     AGYIRMHAQEMDPEVCRQHIALYVNEFSRELGVEGRAAIDALLLRGRAAGLLPPGPSP
                     WR"
     gene            complement(1108..>1692)
                     /gene="mqnB"
                     /locus_tag="VGB85_03380"
     CDS             complement(1108..>1692)
                     /gene="mqnB"
                     /locus_tag="VGB85_03380"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HEY0133087.1"
                     /translation="LESGGLADAIVGVGKVAAAMHTTQLLLTRRPPWLLLIGVCGAYP
                     GSGLDVGALCVVGEETLADEGVALADGFLDLAAMGLGDIGPFVGDIERTAAVAAALGA
                     PVVRGATVSGCSGHDALASAIEARSGAAVESMEGAAVFQVCARLGVPAVQLRCVSNRT
                     GDRARGGWDLHGSIARLHAALRALGGSQGWEGLA"
BASE COUNT          247 a          713 c          544 g          188 t
ORIGIN      
        1 cgagtgacca cgtgatcatg tccggcccgc ccctcaaaaa atttcgctac gagtacccgg
       61 ccagcgaggc ccgcttcctc gccgccccga cggccgaggc cgccgtccgc tacatccgcc
      121 gcgcctaccc gcacaacgcc ctcgacgtgc tcccgaccct gcgagagctc gcccgctggc
      181 ccgagttctg gcgcaccgtc gatgaccagg gcctgctgct cccgcgcgag ccgacctgag
      241 ccgcatgtcc cttcggacat gtccggtggc cgtctcatcg ccagggcgaa ggacccggcg
      301 gcaacagccc cgccgcccgg ccgcgaagca gcaacgcgtc gatcgccgcg cgcccctcga
      361 cgccgagctc gcgcgagaac tcgttgacgt acagcgcgat gtgctgccgg cacacctccg
      421 ggtccatctc ctgcgcgtgc atccggatgt accccgccgg cgcctcggga cgttcccacg
      481 cggccgcgag cgaccggcgg atcccatcct cgacccgctc gagcagcgcc tccccctgcg
      541 cccgctcggc cacgatcacg ccgagcggca gcggcagccc ggtctcggcc tcccacagca
      601 ggccgaggtc cgcgagctgc acgagcccgt ggtccgcgta cgtgaagcgg ctctcgtgga
      661 tgatcagccc ggcgtcgacg ctgcccgagg cgaccgccgg gaggatctcc gagaaggtca
      721 tcgacaccag ctcgcgcggc gccggcagca gcgcgcgcag cagcagcagc gccgtcgtgt
      781 agcgcccggg gatcgccacg cgcatctgcc cgatcccggc cgccccgccc gcgagctccg
      841 gccgcgtgac cagcaacggc ccgcagccgc gcccgagcgc cgcgccggcc gagagcaccg
      901 tgtagcgatc gctgacatgc gccagcgcgt gcacgctgag cttggtgatc ggcagcggat
      961 ctcgcccggc gatcgcccgc acgttcaacg cctcgatgtc ggcgagccac ggcgcgacca
     1021 ccagccccgg caccacgcca tgcacgagcc cgtggaacat gaaggtgtcg ttggggcacg
     1081 gcgagaagcc gatcccgagc gccgcgctca tgccaggccc tcccagccct gcgagccgcc
     1141 cagggcccgc agcgccgcgt gcagccgcgc gatcgaccca tgcagatccc agccgccgcg
     1201 cgcccgatcg ccggtgcggt tgctgacgca gcgcagctgc accgcgggca ccccgagccg
     1261 cgcgcacacc tgaaacacgg cggcgccctc catgctctcg acggccgcgc cgctgcgggc
     1321 ctcgatcgcg ctcgccagcg cgtcgtgtcc cgagcagccc gacaccgtcg cgccgcgcac
     1381 caccggggca ccgagggcag ccgccaccgc cgccgtgcgc tcgatgtcgc cgacgaacgg
     1441 cccgatgtcc ccgagcccca tcgccgcgag atcgaggaag ccgtccgcga gcgcgacgcc
     1501 ctcgtccgcg agcgtctcct cgccgacgac acacagcgcc ccgacgtcga ggccgctccc
     1561 gggatacgcc ccgcacacgc cgatcagcag cagccacggc ggccggcgcg tcagcagcag
     1621 ctgcgtcgtg tgcatcgccg ccgcgacctt gccgacgccg acgatcgcat ccgcgaggcc
     1681 cccgctctcg ag
//
LOCUS       DASWNJ010000182         2979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_674083, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000182 DASWNJ010000000
VERSION     DASWNJ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674083"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VGB85_03385"
     CDS             <1..196
                     /locus_tag="VGB85_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEY0133088.1"
                     /translation="RKLPAVAPHARMLLQVHDELVFEVPEAEVAATTELVRAVMEKPY
                     PLDVPLEVSIGVGPSWGDAH"
     gene            464..1219
                     /locus_tag="VGB85_03390"
     CDS             464..1219
                     /locus_tag="VGB85_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133089.1"
                     /translation="MLPLWMQASLWGLLAGSALVLGALVGYFVRVPKRVVGAIMAFGA
                     GVLTSALAFELVGEARERGGLLPTALGFLAGAAVYACASWLLAHRGARHRKRSTEQPS
                     EDDSGGSGMAIALGAALDGIPESIVVGLGMAGGGTVSLVTVAAIFLSNVPEGLSSAAG
                     MRAAGRPVGYVFAVWGAIAVMSGLASLAGYVFFGGFSPAVIAATTGVAAGAILAMVAD
                     TMLPEAVHETREFTGLITALGFLTALMLSSLET"
     gene            1291..1497
                     /locus_tag="VGB85_03395"
     CDS             1291..1497
                     /locus_tag="VGB85_03395"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0133090.1"
                     /translation="MPQIILEDWNLEAAERRLCVAALELAGNLVGAAKILGITRHALK
                     RRMIKLKIDRPRGTVGGPTPGSAA"
     gene            complement(1504..2154)
                     /locus_tag="VGB85_03400"
     CDS             complement(1504..2154)
                     /locus_tag="VGB85_03400"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY0133091.1"
                     /translation="MSQHDHSHAARARLLAKIPRGYRPWLHLAVPNVYGALVIAACVC
                     SLEGPGPWQWSTLPLVFVAAQGIEWRFHRDLLHRRVPPLQWLYDAHAVSHHAVFVRGA
                     MAIREARELRGVLFPWWAIVALTLLMAPFAWTLAALFGRDVGLLFLVATHAYLMIYEW
                     LHTIYHLPGRSWILRMWPFRGLAEHHAVHHEPRWMPRANFGVTTPLWDWLRGTRRS"
     gene            2286..>2979
                     /locus_tag="VGB85_03405"
     CDS             2286..>2979
                     /locus_tag="VGB85_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133092.1"
                     /translation="MTMGRRTVVGVALLAALWGSAAQAGQIVVMDQTWVHGPDMPDSH
                     FRVPPMDGTPDNWVSPVDYSQGSAWVYLEVHTKPTQQPTKFQVCFEATPTYACTAQSP
                     EYTDVGVYEWETPFANFWSPPGESVDWSLGTNKIACILKDTMNGKPSADNVGDETAAL
                     YMPTEVRMVITLVEPGSVYEPPTPSGEGSTGGESSGGEGTTGGGDSSGGEGMTGNGES
                     SGDELTSMPDTTG"
BASE COUNT          438 a         1056 c         1056 g          429 t
ORIGIN      
        1 ccgcaagctg cccgccgtgg ccccccacgc gcgcatgctg ttgcaggtcc acgacgagct
       61 ggtcttcgag gtccccgagg ccgaggtcgc cgcgaccacc gagctcgtgc gcgccgtgat
      121 ggagaagccc tacccgctcg atgtcccgct cgaggtcagc atcggcgtcg ggccgagctg
      181 gggggacgcg cactagcgtc ttcgcacggg cgcccgcgtc gattcaatat ctgcgcactt
      241 tcgcggtcat ccgcccctat ctgcaccacg aaacgtggcg tgtcgacatc gtcgcgcgcc
      301 ctcgccggga tcgttcgcgg agcctcgggg tgacctccac aaatatgggg gcgtccgcgg
      361 atgctcgggc cgcgccctgc tcgcgaggtc cggtcacgac agtgcaccgg tcgcgtggac
      421 caacgccgtt gcgccggcgc ccgatcgggc cgacaatccc ggtgtgctcc cgctgtggat
      481 gcaggccagc ctctgggggc tgctcgccgg atcggccctc gtcctcggcg cgctggtcgg
      541 ctacttcgtg cgcgtgccga agcgggtcgt cggggcgatc atggcgttcg gtgccggcgt
      601 cctcacctcg gcgctggcct tcgagctggt cggcgaggcg cgcgagcgcg gcggcctgct
      661 gccgacggcg ctcggcttcc tcgccggcgc cgccgtctac gcgtgcgcga gctggctgct
      721 ggcccaccgc ggggcccggc accgcaagcg atcgaccgag cagccgagcg aggacgacag
      781 cggcggcagc ggcatggcga tcgcgctcgg tgccgcgctc gacgggatcc cggagtcgat
      841 cgtggtcggc ctcgggatgg ccggcggcgg caccgtcagc ctcgtcaccg tcgccgcgat
      901 cttcctgtcg aacgtgcccg aggggctgtc gagcgcggcg ggcatgcgcg cggccgggcg
      961 ccccgtcggc tacgtgttcg cggtctgggg cgccatcgcc gtgatgtccg gcctcgcctc
     1021 gctcgccggc tacgtgttct tcggcggctt ctcgccggcg gtcatcgccg cgaccaccgg
     1081 cgtcgccgcc ggcgcgatct tggcgatggt cgcggacacg atgctgcccg aggcggtgca
     1141 cgagacgcgc gaattcaccg gcctgatcac cgcgctcggc ttcctgaccg cgctgatgct
     1201 gtcgtcgctg gagacgtgat cgcgcgctgt ctcttttcgc gcgtatcgct tgatccgcac
     1261 atgatcacgg gcgcgcgcta agccatatgc atgcctcaga ttatcctcga agactggaac
     1321 ctcgaagccg ccgagcgccg cctgtgcgtc gccgccctcg agctcgccgg caacctcgtc
     1381 ggcgccgcga agatcctcgg catcacgcgc cacgccctca agcgccgcat gatcaagctg
     1441 aagatcgatc gcccgcgggg gaccgtcggc ggcccgacgc cgggctccgc cgcatgaccc
     1501 gcctcaggac cgccgggtgc cgcgcagcca gtcccacagc ggggtggtga cgccgaagtt
     1561 ggcgcgcggc atccaccgcg gctcgtgatg gacggcgtga tgctccgcca ggccacgaaa
     1621 cggccacatg cggaggatcc agctgcgccc gggcaaatga tagatcgtgt gcagccactc
     1681 gtagatcatg agatacgcgt gggtggcgac gaggaacagc aggccgacgt cgcggccgaa
     1741 cagcgccgcc agggtccacg cgaacggtgc catcaacagc gtgagggcga cgatcgccca
     1801 ccacgggaac agcacacccc gcagctcgcg ggcctcgcgg atcgccatcg cgccgcgcac
     1861 gaagaccgcg tgatgcgaga ccgcgtgcgc gtcgtacagc cattgcagcg gcggcacgcg
     1921 gcggtgcagc aggtcgcggt ggaaccgcca ctcgatcccc tgcgccgcca cgaacaccag
     1981 cggcagcgtc gaccactgcc agggccccgg accctcgagg ctgcacacgc acgccgcgat
     2041 cacgagggcg ccgtacacgt tgggcaccgc gaggtgcagc cacgggcgat agccccgcgg
     2101 gatcttcgcg aggagccgtg cgcgcgcggc gtggctgtgg tcgtgctgcg acacgggtcc
     2161 atcgtagccg atcgtcgcct gtgacacccg ctggtgtgca gcgcccccac gcgcgtgatc
     2221 gtgtcctggc aacgatcgtg ggatcggcct cgtgctggtg ccggctcgtc gatatcatcg
     2281 cggccatgac gatgggtcga aggacggtgg tcggtgtcgc gttgctcgcg gcgctgtggg
     2341 ggtcggccgc gcaggcgggt cagatcgtgg tgatggatca gacgtgggtg cacgggccgg
     2401 acatgccgga ctcgcacttt cgcgtcccgc cgatggacgg cacgccggac aactgggtca
     2461 gcccggtcga ctactcgcag ggatccgcgt gggtgtacct cgaggtccac accaagccga
     2521 cgcagcagcc gaccaagttc caggtctgct tcgaggcgac cccgacctac gcctgcaccg
     2581 cgcagtcgcc cgaatacacc gatgtcggcg tgtacgagtg ggagacgccg ttcgccaact
     2641 tctggtcgcc gcccggcgag agcgtcgact ggtcgctcgg caccaacaag atcgcgtgca
     2701 tcttgaaaga cacgatgaac ggcaagccct cggccgacaa cgtcggggac gagaccgccg
     2761 cgctgtacat gccgaccgag gtgcgcatgg tcatcacgct cgtcgagccc ggcagcgtgt
     2821 acgagccgcc gacgccctcg ggcgagggga gcaccggcgg cgagagcagc ggcggcgagg
     2881 gcacgaccgg cggcggtgac agcagcggcg gcgagggcat gaccggcaat ggtgagagca
     2941 gcggcgacga gctcaccagc atgcccgaca ccaccggca
//
LOCUS       DASWNJ010000183         1967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_639901, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000183 DASWNJ010000000
VERSION     DASWNJ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639901"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VGB85_03410"
     CDS             complement(<1..470)
                     /locus_tag="VGB85_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133093.1"
                     /translation="MASSEARLSQWLVLGEDQQHQGFIYHWLRGRGVPRGKIRLLPLP
                     AGKGAGEQYVRTQYAPQVVELRRRNYLALALVVVIDADLATVRQRVDELDARSPRQPD
                     ERIALAIPRRNIETWIRFLTAPPVDEHTDYKTRSPDFGATRQAAKLLAGLSTPPA"
     gene            complement(457..1563)
                     /locus_tag="VGB85_03415"
     CDS             complement(457..1563)
                     /locus_tag="VGB85_03415"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0133094.1"
                     /translation="MLRRLYIDNYRSLVNFELTLGPVNLLMGANGSGKSSVFAALAGL
                     RRLVYGAEPVRDCFPASSCTRWQSRRVQTFELAVATPEGSFHYHLELEHAGSESLVKG
                     ERLELDGRPCLASAGEHVEIWGHDSAAPLDRGLVDPSRSSLHLAGDRPKYSAIAAFVR
                     RVGRMHVLSPRPDAMRGWSEGEQPAPSFDLANFASWYRHLAQERAPEMDEAREALRGV
                     LDGFYGLRLRDSGHHEGGRKLVSEWTQGGGKRTSFDFDELSDGQRALIGLYTLLYAGG
                     EDEVTIGVDEPDNFVALPEIQPWLGALSERASLQTLLISHHPEILNLYARDAGLVFRR
                     DDGGPTRVERFTADAEETLTPAELIARGWYRGVQ"
     gene            1677..>1967
                     /locus_tag="VGB85_03420"
     CDS             1677..>1967
                     /locus_tag="VGB85_03420"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEY0133095.1"
                     /translation="MTDETAPLPTFGAVILAGGQSSRMGTPKAWLQWRGQPLLSHIVD
                     LVASVCNHPVVVVGAAGQELPPLHPSAVRIDDPPEFEHRGPLVGLFAGLGVLA"
BASE COUNT          271 a          708 c          676 g          312 t
ORIGIN      
        1 gcaggcggtg tcgagaggcc tgcgagcagc tttgcggcct ggcgcgtggc gccgaagtcg
       61 gggctacggg tcttgtaatc ggtgtgctcg tcgaccggcg gggccgtcaa gaagcggatc
      121 caggtctcga tgttgcgacg tgggatcgcc agcgcgatcc gttcgtccgg ctgacgtgga
      181 ctccgggcgt cgagctcgtc gaccctctgc ctgaccgtcg ccagatcggc gtcgatgacg
      241 accacgagcg caagcgcgag gtaattgcgc cgccgcagct ccacgacctg cggggcgtac
      301 tgcgtgcgga cgtactgctc gcctgcgccc tttccggccg ggagcggcag cagccggatc
      361 ttgccgcgtg ggaccccgcg cccccgcagc cagtgataga tgaagccctg atgctgctgg
      421 tcctcgccga gcaccagcca ctggctcagt cgcgcctcac tggacgccac ggtaccatcc
      481 ccgtgcgatc agctcggcgg gcgtcagtgt ctcttccgcg tcggcggtga accgctcgac
      541 gcgggtcggg ccaccgtcgt cgcggcgaaa gaccaggcca gcgtcgcgcg cgtagaggtt
      601 caagatctcc gggtgatgcg agatcagcaa ggtctgcagc gaggcgcgct cggacagcgc
      661 gccgagccag ggctggatct ccggcaacgc gacgaagttg tccggctcgt cgacgccgat
      721 cgtgacctcg tcctcgccgc cggcgtacag cagggtatag agaccgatca gcgcgcgctg
      781 gccgtccgag agctcgtcga agtcgaagct ggtccgcttg cctccgccct gcgtccactc
      841 gctcaccagc tttcgaccgc cctcgtggtg gccgctgtcc ctgagccgaa gcccgtaaaa
      901 gccgtccagt acgccgcgca gggcctcgcg tgcctcgtcc atctccgggg ctcgctcctg
      961 tgccaggtgc cgataccacg acgcgaagtt ggcgaggtcg aagctcggcg ccggctgttc
     1021 gccctcgctc cagccgcgca tcgcgtcggg cctcggcgag agcacgtgca tccgacccac
     1081 cctgcgcacg aaggccgcga tcgccgagta cttcgggcga tccccggcga ggtgcaggct
     1141 cgatcgagac gggtcgacga gcccgcgatc cagcggcgcg gcgctgtcgt gcccccagat
     1201 ctccacgtgc tccccggcgc tggcgaggca cggccgtccg tcgagctcga ggcgctcgcc
     1261 cttgacgagg ctctcggagc cggcgtgctc gagctcgagg tgataatgaa acgacccctc
     1321 cggggtcgcg acggccagct cgaaggtctg aacccgccgc gactgccagc gcgtgcagct
     1381 cgacgccggg aagcagtcgc gcacgggctc cgcgccgtac acgagccggc gcagcccggc
     1441 gagggccgcg aacaccgagc tcttgccgct cccgttggcg cccatgagca agttgaccgg
     1501 cccgagcgtc agctcgaagt tcacgaggga ccggtaattg tcgatgtaga gccgccgtaa
     1561 cacggatcag ggtccccggc gcatgctacc cggtgcggac cgcgccgtcg accggcggtg
     1621 gggcttgctc cccgggcgcg gcgctgcgat ccttgtcgcc ccccgagcgc ctgcctgtga
     1681 ccgacgagac tgctcctctt cccacctttg gcgccgtcat cctcgctggc ggccagtcct
     1741 cgcgcatggg cacgccgaag gcatggttgc agtggcgcgg acagccgctg ctctcgcata
     1801 tcgtcgatct cgtcgcgtcg gtgtgtaatc accccgtcgt cgtcgtcggc gccgcgggcc
     1861 aggagctgcc gccgctgcac ccctccgcgg tgcgcatcga cgatccgccg gagttcgagc
     1921 atcgcgggcc gctcgtcggg ctgttcgccg gcctcggcgt gctcgcg
//
LOCUS       DASWNJ010000184         6918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_497377, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000184 DASWNJ010000000
VERSION     DASWNJ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6918
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497377"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VGB85_03425"
     CDS             <1..712
                     /locus_tag="VGB85_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133096.1"
                     /translation="LTVSPAADPAKPVSFDIVVADVQLRESVRHKFELPVFGHTAQFA
                     AAPAQKRVGAESARLYNAAHGSARQVGEIPAGARLDILGLAGGWAAVSAGPGRRAWLP
                     ADLLQPAEGKGAGELPARAYALVQPPALTLEPTQQVTTEATVEVRGTARHPLRVHDVL
                     VTVKPAGVGQVEHKVDYEANPAHQGDAARQMTFTASVPLAPGSNQILVTARDAEDVEV
                     TREIWVFRTQDGNLGPAD"
     gene            860..1882
                     /locus_tag="VGB85_03430"
     CDS             860..1882
                     /locus_tag="VGB85_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133097.1"
                     /translation="MFGDVRYFLQGGTPTDLGDAATLVESGLEGHADEYVTLRGTPDV
                     QHAARARLGEKTIGYLRLIEGGGSLYAALPKTDVPASNEFEGTFTGRMRRLADIRMFP
                     WIQQYFDGERIVETRDITREQLLAALDAGKLGADEQLSLSVDQPDARIQLGRTSFPSR
                     AAAEAAVQALGVPYYMPEDQSSAAFYTFIARIPPEQRAAAQATLAAAGIAAPPTDKPD
                     PRIGALVVPFFTTYLVPAGELRHEGDALSFPLGTDGTSPGYIVTDGKLAPRPLVDGRL
                     QIDANLVRAAGRLQPVRVDPNGYIVLVGELPRDQWPTVVLWLVVFSVVAWNLTSLALW
                     WRRKQA"
     gene            1902..3509
                     /gene="cysS"
                     /locus_tag="VGB85_03435"
     CDS             1902..3509
                     /gene="cysS"
                     /locus_tag="VGB85_03435"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY0133098.1"
                     /translation="MTEPAPHLFLYDTLSASKRPLELLEPGRCKLYVCGPTVYDMSHI
                     GHARAAVVPDVLVRFLRASGLEVLFVRNITDVEDKIIKRAATEGITPAEVTTKYTEAY
                     HQDLDALHCLRPDIEPRVTDNIPEIVALIERLFTAGLAYASDGDVYYRVAKFADYGAL
                     SKRNLDDMQAGARVEVNERKESPLDFVLWKAAKPGEPQWDSPWGPGRPGWHIECSAMS
                     LRHLGETFDIHGGGRDLIFPHHENEIAQSQGACGPGTFARHWIHNGFIDFAGEKMSKS
                     LGNFFTIREVTALYLPEALRYFLLGVHYRSGVNFDVEVPCPSCGQLLDPEQQQAGACP
                     QCAAPLELARVRAHIRFPGLEEADERVAYVYDTLARCQRALTSATPDPAAEDAPQNTV
                     AIAPSIAGMLPAFVAALRDDLNTAAAIAVLSEPLREVNRLLEFKKKAEIAGRGPTLTR
                     FVASMRTVSRYLGLFAEDPALYLRERRDRKAARLRLDVAEVERLLRMREEARAKKDWK
                     EADRLRAELGLLGVTLQDSAEGSTWTL"
     gene            3581..4438
                     /locus_tag="VGB85_03440"
     CDS             3581..4438
                     /locus_tag="VGB85_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0133099.1"
                     /translation="MTLRLFGAGGAFSRRYGTTCSMVTLRSGQRWLIDCGRQAPDQLH
                     AAGFAWHDITGQIVTHVHGDHVYGMEDFAFIRFYETRGPDLASSLGGAMPRLIAHSAV
                     RAEIWETLAPSLRYLNDGRGNCQAGTLAHYFDIIDAVVIEPPRNNTWHHAEHFVSDDL
                     SIVARETLHVPGKPSCSLEFLVEEDRGVQRIAWWSGDCTVDPELLISLEPRTSIYFHD
                     CTFVDYPGQVHGFFEKLEALPASVRHKMVLMHHEDNLDQNRTRVEAAGFRVAMPGQIY
                     DLVSGQRMA"
     gene            complement(4441..4971)
                     /gene="moaB"
                     /locus_tag="VGB85_03445"
     CDS             complement(4441..4971)
                     /gene="moaB"
                     /locus_tag="VGB85_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HEY0133100.1"
                     /translation="MSERAFIPVQVAVVTVSDTRTLETDRSGQTIADLLTSAGHVVIR
                     RDIVRDNREQIREALRGHIADREVDVIITTGGTGVTPRDVTPEALAPLVTKPIPGFGE
                     LFRWISYAEIGTSTIQSRAEAALCDTTYVFLLPGSTGAVKTAMEKILLAQLDHRHRPC
                     NFVELLPRIHGEREDG"
     gene            complement(4982..5776)
                     /locus_tag="VGB85_03450"
     CDS             complement(4982..5776)
                     /locus_tag="VGB85_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133101.1"
                     /translation="MLVLAACPTKPPGQDTGDETGTDSDTGDTGGPSEIPAVCARWVG
                     CTAEIDPGTAAMMAGQYGEGGTCWQEDDAGQAGCIATCDVRLREYGDAFPDLEVCRYD
                     DILGGVEFTLGEAVFDPNDPLAEPTFRELEDGDTVQIVRGGQGLLMLPLAVRGRGFEV
                     PADPTDWDNPRMPHINLWMDIEGFNVGFGGHFARVPDYPIGFEPIDDMGTLEHLYIAV
                     LVPDAIDDPALLTNQPGTIRAELRTYMQPTVARQLSFVVAPTIQGE"
     gene            6039..>6918
                     /locus_tag="VGB85_03455"
     CDS             6039..>6918
                     /locus_tag="VGB85_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133102.1"
                     /translation="MTALSAHRRRVHLLAALSLLLLAGCYSGDFFDRLVDPTATAAFR
                     ITRLVLIDPHTYIGNTVQCEDSTAALNEIFAKNIASFDVNTTLVLHPLDPTVTSGAKM
                     EIVPANCVPGGELVNCTDKDVDIAEIVSADFNNSVGGTCGNPVAMSLNTAYQGAGNEP
                     LHQAMSPCFLSAPIPKLGLKLALDVPLPLSNVQIYASYALDDKPHRLVSGVLFGFVPA
                     SVAMTPIGELGDGPNDGPFALWSNLAGGGACQPSAMFTDIDSAAPEDGEWMYFNFTAE
                     LVAWSPENDLPGTTGEP"
BASE COUNT         1044 a         2680 c         2178 g         1016 t
ORIGIN      
        1 cctgaccgtc agcccggcgg ccgacccggc caagccggtc tccttcgaca tcgtcgtcgc
       61 cgacgtccag ctgcgcgaga gcgtgcgcca caagttcgag ctcccggtct tcggccacac
      121 cgcccagttc gcggcagcgc cagcccaaaa gcgcgtcggc gccgagtccg cgcgcctcta
      181 caacgccgcc cacggcagcg cccgccaggt cggcgagatc ccggccggcg cgcgcctcga
      241 catcctcggc ctcgccggcg gctgggctgc cgtctcggcc ggccccggcc gccgcgcctg
      301 gctcccggcc gacctgctcc agccggccga gggcaaaggc gccggtgaat tgccagcccg
      361 cgcctacgcg ctcgtccagc cgcccgccct gaccctcgag ccgacgcaac aggtcaccac
      421 cgaggccacc gtcgaggtgc gcgggaccgc gcgtcacccc ttgcgggtgc acgacgtgct
      481 cgtgacggtc aagccggccg gtgtcggcca ggtcgagcac aaggtcgact acgaggccaa
      541 ccctgcgcat cagggcgacg ccgcccggca aatgacgttc actgccagtg ttccgctcgc
      601 gccgggctcc aaccagatcc tcgtcaccgc gcgcgacgcc gaggacgtcg aggtcacccg
      661 agagatctgg gtgtttcgca cgcaggacgg gaacctcggg cccgcggatt agtcctgggg
      721 ctggaccgtg ataagacgag gcaccgtggt cgaaccagag caacctgcgg acgaaatctc
      781 ctcccccgcg ccgcgtcgtc ggcgctcccc cctcatcgac gtcgcggtcg ccctgttcgg
      841 cggctacctc ctcgtgacca tgttcgggga cgtccgctac ttccttcagg gcggcacccc
      901 gacggacctc ggcgacgctg ccaccctcgt cgagtccggt cttgaaggcc acgccgacga
      961 gtacgtcacc ctgcgcggca cgccggacgt ccagcacgcc gcgcgcgccc gcctcggcga
     1021 gaagacgatc ggctacctgc gcttgatcga gggcggcggc tcgctgtacg ccgcgctgcc
     1081 gaagaccgac gtgcccgcca gcaacgagtt cgagggcacc ttcaccggcc gcatgcgtcg
     1141 cctcgccgac atccgcatgt tcccgtggat ccagcaatac ttcgacggcg agcgcatcgt
     1201 cgagacccgc gacatcaccc gcgagcagct gctcgccgcc ctcgatgccg gcaagctcgg
     1261 cgccgacgag cagctgagcc tcagcgtcga ccagcccgac gcacgcatcc agctcggtcg
     1321 caccagcttc ccctcgcgcg ccgccgccga ggccgccgtg caggccctcg gcgtcccgta
     1381 ctacatgccc gaggaccagt cctcggccgc gttctacacc ttcatcgccc gcatcccgcc
     1441 cgagcaacgc gccgccgccc aggccacgct cgcggccgcc ggtatcgccg cgccgccgac
     1501 cgacaagccc gacccccgca tcggcgcgct cgtcgtcccg ttcttcacga cctacctcgt
     1561 gcctgccggc gagctgcgcc acgagggcga cgcgctgagc ttcccgctcg gcaccgacgg
     1621 caccagcccc ggctacatcg tcaccgacgg caagctcgcc ccgcgcccgc tcgtggatgg
     1681 ccgcctgcag atcgacgcga acctcgtccg cgccgccggc cgcctgcagc cggtccgggt
     1741 cgacccgaac ggctatatcg tcctcgtcgg cgagctgccg cgagaccagt ggccgaccgt
     1801 ggtgttgtgg ctcgtcgtgt tctcggtcgt cgcctggaac ctgaccagcc tggccctttg
     1861 gtggcgccgt aagcaggcat agggcacact gcggctccgc catgacggag cccgcaccgc
     1921 acctcttcct gtacgacacc ctgagcgcca gcaagcgccc gctcgagctg ctcgagcccg
     1981 gccgctgcaa gctctacgtg tgcggcccga ccgtctacga catgagccac atcggccacg
     2041 cgcgcgccgc cgtcgtgccc gacgtgctgg tgcgattcct gcgcgcgtca ggcctcgagg
     2101 tgttgttcgt ccgcaacatc accgacgtcg aggacaagat catcaagcgc gcggcgaccg
     2161 agggcatcac gcccgccgag gtcaccacca agtacaccga ggcctatcat caggacctcg
     2221 acgcgctgca ctgcctgcgc cccgacatcg agccgcgcgt caccgataac atcccggaga
     2281 tcgtggccct gatcgagcgc ctgttcaccg ccggcctcgc ctacgcgtcc gacggcgacg
     2341 tgtactaccg cgtcgcgaag ttcgcggact acggcgcgct cagcaagcgc aacctcgacg
     2401 acatgcaggc cggcgcgcgc gtcgaggtca acgagcgcaa ggagagcccg ctggacttcg
     2461 tgctgtggaa ggcggccaag cctggcgagc cgcagtggga cagcccgtgg ggtcccggcc
     2521 ggcccggctg gcacatcgag tgctcggcga tgagcctgcg ccacctcggc gagaccttcg
     2581 acatccacgg cggtggccgc gatctgatct tcccccacca cgagaacgag atcgcgcaat
     2641 ctcagggcgc ctgcggcccc ggcaccttcg cgcggcactg gatccacaac ggctttatcg
     2701 acttcgccgg cgagaagatg tccaagagcc tcggcaactt cttcacgatc cgcgaggtca
     2761 ctgccctgta tctcccggag gccctgcgct acttcctgct cggcgtgcac taccgcagcg
     2821 gcgtcaactt cgacgtcgag gtcccgtgcc cgagctgcgg ccagctgctg gaccccgagc
     2881 agcagcaggc cggcgcctgc ccgcagtgcg ccgccccgct cgagctcgcg cgcgtgcgcg
     2941 cgcacatccg cttccccggc ctcgaggagg ccgacgagcg cgtcgcgtac gtctacgaca
     3001 cgctcgcccg ctgccagcgc gccctgacca gcgccacgcc cgaccccgcc gccgaggacg
     3061 cgccgcaaaa caccgttgcg atcgcgccgt cgatcgccgg catgttgccg gcgttcgtcg
     3121 cggccctgcg cgacgacctc aacaccgccg ccgcgatcgc cgtcctgagc gagcccctgc
     3181 gcgaggtgaa ccgcctgctc gagttcaaga aaaaggccga gatcgccggt cgcggcccga
     3241 cgctgacccg ctttgtcgcc agcatgcgca cggtttcgcg gtatctcggc ctgttcgcgg
     3301 aggatccggc cctgtatctg cgcgagcgcc gggaccgcaa ggccgcgcgc ctgcgcctcg
     3361 acgtggccga ggtcgagcgg ctcctgcgga tgcgggagga ggcgagggcc aaaaaagact
     3421 ggaaagaagc cgatcgactg cgagctgagc taggcttgct gggcgtgacc ctgcaggact
     3481 ctgccgaggg ctcgacctgg accctgtaat tcctccctcc gggcgcccgc cccggccgtt
     3541 caacctctga ctcgctcgcg tgtctgatcg cagccaaacc atgacgctcc gcctgttcgg
     3601 cgctggcgga gcgttctcgc ggcgctacgg caccacgtgc agcatggtca ccctgcgctc
     3661 gggtcagcgc tggctcatcg actgcggacg ccaggccccc gaccagctcc acgccgccgg
     3721 cttcgcctgg cacgacatca ccgggcagat cgtcacccac gtccacggcg accacgtcta
     3781 tggcatggag gacttcgcct tcatccgctt ctacgagacg cgcggccccg acctcgccag
     3841 ctccctcggc ggcgcgatgc cgcgactgat cgcccacagc gccgtgcggg ccgagatctg
     3901 ggagaccctc gccccgtccc tgcgctacct caacgacggc cgcggcaact gtcaggccgg
     3961 cacgctcgcc cactacttcg acatcatcga cgccgtcgtc atcgagcccc cgcgcaacaa
     4021 cacctggcac cacgccgagc acttcgtctc cgacgacctc tcgatcgtcg cccgcgagac
     4081 cctccacgtc cccggcaagc cgtcctgttc gctcgagttc ctcgtcgagg aggaccgtgg
     4141 cgtccagcgc atcgcctggt ggagcggcga ctgcaccgtc gaccccgagc tgctaatcag
     4201 cctcgagccg cgcacctcga tctacttcca cgactgcacc ttcgtcgact accccggcca
     4261 ggtccacggc ttcttcgaga agctcgaggc cctgcccgcg agcgtgcgcc acaagatggt
     4321 cctgatgcac cacgaggaca acctcgacca gaaccgtacg cgcgtcgagg cggccggctt
     4381 tcgcgtcgcc atgcccgggc agatctacga tctcgtgtcc ggtcagcgca tggcctgact
     4441 tcagccgtcc tcgcgctcgc cgtggatccg cggcagcagc tcgacgaagt tgcagggccg
     4501 atgccggtga tcgagctgcg cgagcaggat cttctccatc gccgtcttga ccgcgccggt
     4561 cgagcccggc agcaagaaca cgtaggtcgt gtcgcacagc gcagcctcgg cgcgcgactg
     4621 aatggtcgag gtcccgatct ccgcgtagct gatccagcgg aacagctcgc cgaagccggg
     4681 gatcggcttc gtgaccagcg gcgcgagggc ctcgggggtc acgtcgcggg gcgtcacgcc
     4741 ggtgccgccg gtggtgatga tgacatcgac ctcgcggtcg gcgatatggc cacgcagggc
     4801 ctcgcggatc tgctcgcggt tgtcgcggac gatgtcacga cggatcacca cgtggccggc
     4861 ggaggtcagc aggtcggcga tcgtctggcc ggatcgatcg gtctcgaggg tgcgggtgtc
     4921 ggagaccgtg acgacggcga cctgcaccgg gatgaacgcg cgctcgctca tgcccgcaaa
     4981 gctactcccc ctggatcgtc ggggccacca cgaagctgag ctggcgggcg accgtcggtt
     5041 gcatgtacgt gcgcagctcg gcccggatcg tgcccggctg gttcgtcagc agcgcggggt
     5101 cgtcgatcgc gtccggcacc agcacggcga tgtacaggtg ctcgagcgtg cccatgtcgt
     5161 cgatcggctc gaagccgatc gggtagtccg gtacccgcgc gaagtgcccg ccgaagccga
     5221 cgttgaagcc ctcgatgtcc atccagaggt tgatgtgcgg catgcgcggg ttgtcccagt
     5281 cggtcgggtc cgccggcacc tcgaagccgc gcccgcgcac cgccagcggc agcatcaaca
     5341 ggccctgacc accacgcacg atctgaaccg tgtcgccgtc ctcgagctcg cgaaacgtcg
     5401 gctccgccag cgggtcgttc gggtcgaaca cagcctcgcc gagcgtgaac tccaccccgc
     5461 cgaggatgtc gtcgtagcgg cacacctcga ggtccgggaa cgcgtccccg tactcgcgca
     5521 gccgcacgtc gcacgtagcg atgcagccgg cctgcccggc gtcgtcctcc tgccagcagg
     5581 tccccccctc gccgtactgg ccggccatca tcgccgccgt gccggggtcg atctccgccg
     5641 tgcagccgac ccaccgcgcg cacacggcgg ggatctcgct ggggcccccg gtgtcgcccg
     5701 tgtcggagtc cgtcccggtc tcgtcgccgg tgtcctgacc gggcggcttc gtcggacagg
     5761 cggcgagcac cagcatcgtc cacagcgccg ctgccggcgt acgcgaggct cgcgacaaat
     5821 taagaaaaac gcgcgacata tgccgtccat ggtgggccac cgcggctcgc ccggcaaccc
     5881 tgctcggatc gagccatgca gcctggcagc ccgtggtggc cgatccacgc ggacgcctgc
     5941 cgtggcgcac acaggcccac gggcgggggc gtcgcgggag gattctcccg gcgcgcgcgt
     6001 ccgggcggtc gtggcgatcg gctatcatgg cggcccccgt gaccgccctc tccgcccacc
     6061 gccgccgcgt ccacctgctc gcggctctct cgctgctgct gctggccggc tgttacagcg
     6121 gcgacttctt cgatcgcctg gtggacccga ccgcgaccgc cgcgtttcgc atcacgcgtc
     6181 tggtcctgat cgacccgcac acgtacatcg gcaacaccgt ccagtgcgag gactcgaccg
     6241 ccgcgctcaa cgagatcttc gccaagaaca tcgccagctt cgacgtcaac acgaccctcg
     6301 tcctgcaccc gctcgacccg accgtcacct ccggggccaa gatggagatc gtgcccgcga
     6361 attgtgtgcc gggcggcgag ctcgtcaact gcaccgacaa ggacgtcgac atcgccgaga
     6421 tcgtgtcggc cgacttcaac aattcggtcg gaggcacctg cggcaacccg gtcgccatgt
     6481 cgctcaacac cgcctatcag ggcgccggca acgagccgct gcatcaggcg atgagcccct
     6541 gcttcctcag cgcgccgatc cccaagctcg gcctcaagct cgcgctcgac gtgcccctcc
     6601 cgctgtccaa cgtccagatc tacgcctcct acgcgctcga cgacaagccg caccgcctcg
     6661 tctccggcgt gctgttcggc ttcgtccccg cgagcgtcgc catgacaccg atcggcgagc
     6721 tcggcgacgg gcccaacgac gggcccttcg ctctgtggtc caacctcgcc ggcggcggcg
     6781 cgtgccagcc gtccgccatg ttcaccgaca tcgacagcgc cgcccccgag gacggcgagt
     6841 ggatgtactt caacttcacc gccgagctcg tcgcctggtc gcccgagaac gacctccccg
     6901 gcaccaccgg cgagccga
//
LOCUS       DASWNJ010000185         1646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_390515, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000185 DASWNJ010000000
VERSION     DASWNJ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390515"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..509
                     /locus_tag="VGB85_03460"
     CDS             150..509
                     /locus_tag="VGB85_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133103.1"
                     /translation="MIRRRRLIITPGPGRTLPDPEDPSLPQVSPHQPESEPPVPLPDG
                     PQRPPAREDPPDEPREGRGARPAAGVEPWLRGLGVVADQDARRAWLEPHLWAALRARV
                     DATAAERRARWIQPGFR"
     gene            576..>1646
                     /locus_tag="VGB85_03465"
     CDS             576..>1646
                     /locus_tag="VGB85_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0133104.1"
                     /translation="MTSHRLQPARAAGYMARRLKNAVHMARRNDIRNLAIIAHVDHGK
                     TTLVDTLLTAAGAFDRNEGQIERAMDSMDLEKERGITITSKPTSLTYKGVRINLVDTP
                     GHADFGGEVERVLNMVDGVLVVVDAFEGPMPQTRFVTEKAVARGLKILLVVNKIDRPG
                     AEPHKTVDAVFDLLVNLGASEEQTDFPVIFCSARERYAMMDPADEKQDMTKILDFIIE
                     RAPGPEITEGPLAMWVSTLDYDSFLGYVAIGRIQSGTMKVGERLALVRPARAGAPAGS
                     PSSVLAESFRVRKLLGFQGLRRFERETAEAGDIVALSGMESLEVGDSITTEDPATRTV
                     FPRLDVDPPTMTMRFRVNDGPFA"
BASE COUNT          266 a          610 c          532 g          238 t
ORIGIN      
        1 cacggccggc ctgcgcgtct acacgcaaga tgttgcgaaa gtgcgccgaa ttccagcgaa
       61 ttgagggtgt gagccaggac tgataaagcg ttcagtgcgc tgctggatcc agcttgagct
      121 gagcgtctgt tcagttactt actggatcca tgatccgccg tcgccgcttg atcatcacgc
      181 ccggacctgg ccgcaccctg cccgaccccg aggatccttc tttgccgcag gtttcgccgc
      241 accagccgga gtccgagccg ccggtaccgc tgccggacgg cccgcagcga ccgcctgcgc
      301 gcgaagatcc cccggacgag ccccgcgagg gacgtggcgc ccggccggct gcgggcgtgg
      361 agccgtggct gcgggggctc ggggtggtcg ccgatcagga cgcgcggcgg gcctggctcg
      421 agccgcacct gtgggccgcg ctgcgggcca gggtcgatgc gaccgccgcc gaacgccggg
      481 cccgctggat ccagcccggt ttccgctgac ccgaagacgt cgggctccgc gccgtgcccc
      541 ggcaagtcac gccgcggaga tcgtgtccct tcgccgtgac ctcgcaccgc ttgcaaccgg
      601 cccgcgccgc gggatacatg gcgcggcgcc tgaaaaacgc cgtccacatg gcccgccgca
      661 acgacatccg aaacctcgca atcatcgccc acgtcgacca cgggaagacc accctcgtcg
      721 acaccttgct caccgccgcg ggggccttcg accgcaacga ggggcagatc gagcgcgcca
      781 tggacagcat ggacctcgag aaggagcgcg gcatcaccat caccagcaag ccgaccagcc
      841 tcacctacaa gggtgtgcgc atcaacctgg tcgacacgcc ggggcacgcc gacttcggcg
      901 gtgaggtcga gcgtgtgctc aacatggtcg acggcgtgct cgtcgtcgtc gacgccttcg
      961 agggaccgat gccgcagacg cgcttcgtca ccgagaaggc cgtcgcgcgc gggctcaaga
     1021 tcctgctcgt cgtcaacaag atcgaccgtc ccggcgccga gccgcacaag accgtcgacg
     1081 ccgtcttcga cctgctcgtc aacctcggcg ccagcgagga gcagaccgac ttcccggtca
     1141 tcttctgcag cgcccgcgag cgctacgcga tgatggatcc tgccgacgag aagcaggaca
     1201 tgaccaagat cctcgacttc atcatcgagc gcgcccccgg accggagatc accgagggtc
     1261 cgctggcgat gtgggtctcg accctcgact acgactcctt cctcggctac gtcgccatcg
     1321 gcaggatcca gagcggcacc atgaaggtcg gcgagcgcct cgcgctggtc cgcccggccc
     1381 gcgccggcgc cccggccggc agcccgtcct cggtgctcgc cgagtcgttc cgcgtgcgca
     1441 agctgctcgg cttccagggc ctgcgccgct tcgagcgcga gaccgccgaa gccggtgaca
     1501 tcgtcgcgct gtcgggcatg gagagcctcg aggtcggcga ctcgatcacc accgaagacc
     1561 cggccacgcg caccgtgttc ccgcggctcg acgtcgaccc gccgaccatg acgatgcgct
     1621 ttcgcgtcaa cgacggcccg ttcgcc
//
LOCUS       DASWNJ010000186         5441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_639952, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000186 DASWNJ010000000
VERSION     DASWNJ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639952"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1626
                     /locus_tag="VGB85_03470"
     CDS             <1..1626
                     /locus_tag="VGB85_03470"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0133105.1"
                     /translation="IRQVLAAWEAGASEVPLLGGGVAPLPHDWMARYGPQVALLLASR
                     GSDGEVAPHARPALADLYAALETPAPPSLAGLAALLRDNLALPRAKLPADLTAELRSY
                     QRRGVEWLSLLRDAGLGAMLADDMGLGKTLQCLCTLQGRCLIVAPTSVLYNWAAEAAR
                     FRPGLRVCTYHGPRRKLDPDADLVLTSYAILRLDAEALATEAWDAVVLDEAQTIKNPD
                     SQVARAAYGLTKARWRVTLTGTPVENRLEELWSQFHFIMPGLLGGRRDFADRHANPIA
                     DGVPGAAGRLRTILKPFLLRRRKQEVAKELPPRTEVVLRCTLDDRERNIYDAIRAATE
                     KEVVEALESGGGVLMALEALLRLRQAACHAGLVPGQHAERSSKLDLLQDTLDEAVAEG
                     HKALVFSQWTGLLDRVEPYLRRANLDFVRLDGSTRDRGAVVDKFQAEGGPPIMLVSLK
                     AGGTGLNLTAADHVFMLDPWWNPAVEDQAADRAHRIGQTRPVLVHKLVAEDTVEARIL
                     ELQQRKRALADAALAGADAATSLTREDLLALLR"
     gene            complement(1633..2346)
                     /locus_tag="VGB85_03475"
     CDS             complement(1633..2346)
                     /locus_tag="VGB85_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133106.1"
                     /translation="MRLFHSILCVAALVTACNDEGGTGETAPLGTTGAPDNDTTGAPD
                     NDTSGSSGEVETQACVKATVAVVNECQETFCPPVLLHLTVDNAGGAAGLVRQDWPIDA
                     IGYRHNAPELRVDLTFAEALGDDTSDAARTTHFAELDLTFEFPTLGDSTPLGDSWTLR
                     DFFGRIPDITDPAQFESFAITGGRLKATFHGELSSVQMTVYSDAEQCVDGDIAGRCDC
                     TYDGFTLPVIVDLDLSIDD"
     gene            complement(2460..2861)
                     /locus_tag="VGB85_03480"
     CDS             complement(2460..2861)
                     /locus_tag="VGB85_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133107.1"
                     /translation="MKTSTITRILVAFALVGLPACDGGGDTKKTDTKTVAKTDAAKPA
                     VKVETKTDTKVADNAAKPDDTKEQPIVDPNLEKIKLAATVAREISGAPDQADEILGKH
                     GLDRDKLDALMFEIAGDPELTQAYMAARRDS"
     gene            complement(2885..3895)
                     /locus_tag="VGB85_03485"
     CDS             complement(2885..3895)
                     /locus_tag="VGB85_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133108.1"
                     /translation="MNKLCFTFLTLALGAPMLANASEQAATTADGAKTTAAASGSAAV
                     SVNVKVSDQDALGDDILAVINLPIAAADAREAGVEETELKAALEVTRETGLSAGDASE
                     VVAEEAEQTRARGVKKGFGQWVKMQVAAGLRGKALAAKIKARKADTAELDEKKIAELT
                     TKLEKQRELNRAWRAKAQEKRTELIAKGKKKVILHEERNEKLKAKIQAAQGRNEERGD
                     AVEGRIKAIDAKIAAASGDEKKALEDEKKRLERALKTNEKIEDRLEKAEDKAEKKDDK
                     LDKAGDKLDKLTDKAADKAEKKDARLDAKADAKADAKADAKAATGGARTRTPGPAAPA
                     PQ"
     gene            complement(3976..4536)
                     /gene="tsaA"
                     /locus_tag="VGB85_03490"
     CDS             complement(3976..4536)
                     /gene="tsaA"
                     /locus_tag="VGB85_03490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEY0133109.1"
                     /translation="MQRRKPEQDHTSEHADAHRQPFCFTPIGVLRSPYTERFGTPRQP
                     PITTQVKGDAMLPARIELLPDRGLEDGLEGLADFDRIWCLFVFHLNRGHSMTVRSPRD
                     PNGSRGLFATRSPHRPNNIGLSCLRLTAIEGRVLHVHGVDILDHTPVLDIKPYVPYAD
                     AFPGARQGWLEDRIPDEPDHYEPPAT"
     gene            4685..>5441
                     /locus_tag="VGB85_03495"
     CDS             4685..>5441
                     /locus_tag="VGB85_03495"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0133110.1"
                     /translation="MGVFAWSLAVGCGDSGSAGETTTSTSATTTVTSASGTGEPGTTG
                     DVVTTGDTPTGDTPTGGNEGSATGTTTGETGTTGVDSSTGDIEGTTAIPDPVCGDAVL
                     DPGEGCDDGEMNADDGACTMQCQPATCGDGLVQAGVEQCDEGAANADDGACTSQCVPA
                     TCGDLLVQTGVEQCDDGNADDTDACVAGCKNAACGDGLVGPGELCDDGNQNDDDSCTN
                     ACASPNCGDGKLQMAQGEECDDGNALDSDACLST"
BASE COUNT          747 a         1846 c         1966 g          882 t
ORIGIN      
        1 atccggcagg tgctcgcggc gtgggaggcg ggcgcctccg aggtgccctt gctcggcggg
       61 ggcgtcgccc cgctgccgca cgactggatg gcccgctacg gcccgcaggt cgccctgctg
      121 ctggcctcgc gcgggtccga cggcgaggtc gccccgcacg cgcgcccggc cctcgccgac
      181 ctgtacgccg cgctcgagac cccggcgccg ccgagcctcg ccggtctcgc cgcgctgctg
      241 cgcgacaacc tcgcgctgcc gcgcgcgaag ctgccggccg acctcaccgc cgagctgagg
      301 tcgtaccagc gccgcggcgt cgagtggctg tcgctgctgc gcgacgccgg cctcggggcg
      361 atgctcgccg acgacatggg cctcggcaag acgctgcaat gtctgtgcac gctgcagggc
      421 cgctgcctga tcgtcgcgcc gacgagcgtt ctatataact gggccgccga ggccgcccgc
      481 ttccggcccg gcctgcgcgt gtgcacatat cacggcccgc gccgcaagct cgaccccgac
      541 gccgacctcg tgctcaccag ctacgcgatc cttcgcctcg acgccgaggc gctcgcgacc
      601 gaggcgtggg acgccgtcgt gctcgacgag gcgcagacga tcaagaaccc cgacagccag
      661 gtcgcgcgcg cggcctacgg gctgacgaag gcccgctggc gcgtgacatt gacaggcacg
      721 ccggtcgaga accgcctcga ggaattgtgg agtcagttcc acttcatcat gccgggcctg
      781 ctcggtggcc gccgcgactt cgccgaccgc cacgccaacc cgatcgccga cggcgtgccc
      841 ggagccgccg gtcgcctgcg gacaatttta aagcccttcc tgctgcgcag acgaaagcag
      901 gaggtggcga aggagctgcc gccgcgcacc gaggtcgtgc tgcgctgcac cctcgacgac
      961 cgcgagcgca acatctacga cgcgatccgt gccgccaccg aaaaggaggt cgtcgaggcg
     1021 ctcgagtccg gcggcggcgt gctgatggcg ctcgaggccc tgctgcgcct gcgccaggcc
     1081 gcgtgccacg ccgggctcgt gccgggccag cacgccgagc gctcgtcgaa gctcgacctg
     1141 ttgcaggaca cgctcgacga ggcggtcgcc gagggccaca aggcgctcgt attctcgcag
     1201 tggaccgggc tgctcgaccg cgtcgagccg tacctgcgcc gcgccaacct cgacttcgtg
     1261 cgcctcgacg gcagcacgcg tgaccgcggc gcggtggtcg acaaattcca ggccgagggt
     1321 ggcccgccga tcatgctggt ctcgctcaag gccggcggca ccggcctcaa cctgaccgcc
     1381 gccgatcacg tgttcatgct cgacccctgg tggaaccccg ccgtcgagga tcaggcggcg
     1441 gaccgcgctc accgcatcgg gcagacccgg cccgtgctcg tgcacaagct cgtcgccgag
     1501 gacaccgtcg aggcgcggat cctcgagctg cagcagcgca agcgggcgct cgcggacgcg
     1561 gcgctcgcgg gcgcggacgc ggcgacctcg ctgacccggg aggacctgct ggcgctgctc
     1621 cggtgagtga agtcaatcgt cgatcgagag gtcgaggtcg acgatcaccg gcagcgtgaa
     1681 gccgtcatag gtgcagtcgc atcgaccggc gatatcaccg tcgacacact gctcggcgtc
     1741 cgaatacacc gtcatctgca cgctgctcag ctcgccgtga aacgtggcct tcaggcgccc
     1801 gcccgtgata gcgaacgact cgaactgcgc gggatcggtg atgtcgggga tgcgcccaaa
     1861 aaagtcacgg agcgtccagc tgtcgcccag cggcgtactg tcgcccagcg tcggaaattc
     1921 gaaggtgagg tcgagctccg cgaaatgggt cgtgcgcgcc gcgtccgagg tatcgtcacc
     1981 gagggcctcg gcgaaggtga ggtcgaccct gagctcgggc gcgttgtggc gatagccgat
     2041 cgcatcgatg ggccaatcct ggcgcacgag gcccgccgcc ccgcccgcgt tgtcgacggt
     2101 caggtgcaac agcactggcg ggcagaacgt ctcctggcac tcgttgacca cggcgaccgt
     2161 ggccttgaca cacgcctggg tctcgacctc gcccgagctc ccggacgtat cgttatcggg
     2221 cgcgccggtc gtgtcattgt cgggcgcgcc ggtcgtgccg aggggtgcgg tctcacctgt
     2281 cccgccctca tcgttgcaag cggtcaccag cgctgccaca cagaggatcg agtggaacaa
     2341 acgcatcctc ccaggatagg ccggcgtctg cgtttgtgca aaccgtccgg caggcgcctt
     2401 aacgaagaat ccaggcgcca cccgggggcg acgcctggat cttggtgcga cggacaggct
     2461 cagctgtcgc ggcgagcggc catgtaggcc tgggtcagct cggggtcgcc ggcgatctcg
     2521 aacatgaggg cgtcgagctt gtcgcggtcg aggccgtgct tgccgaggat ctcgtcggcc
     2581 tggtcgggcg cgcccgagat ctcgcgggcg acggtcgcgg cgagcttgat cttctcgagg
     2641 ttcgggtcga cgatcggctg ctccttggtg tcgtccggct tcgccgcgtt atcggcgacc
     2701 ttggtgtcgg tcttggtttc gaccttgacg gccggcttgg cggcgtccgt cttggcgacc
     2761 gtcttggtgt cggtcttctt ggtgtcaccg ccgccgtcgc aggcggggag accgacgagg
     2821 gcgaaggcga cgaggatgcg cgtgatggtt gaagtcttca tggtgcgttc tcctggtcgt
     2881 cggatcactg gggggcgggc gcggcggggc cgggcgtgcg ggtcctggcg ccgccggtcg
     2941 cggccttggc atcggccttg gcgtcggcct tggcgtcggc cttggcgtcg agcctggcgt
     3001 ccttcttctc ggccttgtcg gcggccttgt cggtcagctt gtcgagcttg tcgccggcct
     3061 tgtcgagctt gtcgtccttc ttctcggcct tgtcctcggc cttctcgagg cggtcctcga
     3121 tcttctcgtt ggtcttgagg gcccgctcga ggcgcttctt ctcgtcctcg agcgccttct
     3181 tctcgtcgcc gctggcggcg gcgatcttgg cgtcgatcgc cttgatgcgg ccctcgacgg
     3241 cgtcgccgcg ctcctcgttg cggccctgcg cggcctggat cttggccttg agcttctcgt
     3301 tgcgctcctc gtgcaagatg accttcttct tgcccttggc gatcagctcg gtgcgcttct
     3361 cctgggcctt ggcgcgccag gcacggttga gctcgcgctg cttctcgagc ttcgtggtca
     3421 gctcggcgat cttcttctcg tcgagctcgg cggtgtccgc cttgcgcgcc ttgatcttgg
     3481 cggcgagcgc cttgccgcgc aggccggcgg cgacctgcat cttgacccac tggccaaagc
     3541 ccttcttgac gccgcgggca cgggtctgct cggcctcctc ggcgacgacc tcgctcgcgt
     3601 cgccggcgct gagcccggtc tcacgcgtga cctcgagcgc agccttgagc tcggtctcct
     3661 cgacgccggc ctcgcgggcg tcggcggcgg cgatcggcag gttgatgacg gccaggatgt
     3721 cgtcaccgag cgcgtcctgg tcagagacct tgacgttgac ggagacagcg gccgagccgg
     3781 aggccgcggc cgtggtcttg gcgccgtcgg cggtcgtggc ggcttgctcg ctggcgttgg
     3841 ccagcatggg cgcgcccagt gccagggtca ggaaggtaaa acacagcttg ttcatcggga
     3901 ctctccgcgc ccaggataaa cgatgagctg acgaaaatgt tgggggcggt gggccccttt
     3961 cacctgtcca ttcgctcagg tcgccggggg ttcgtaatga tcgggttcgt caggaatgcg
     4021 atcttccagc cagccctggc gggcgccggg gaaggcgtct gcatagggga catagggctt
     4081 gatatcgagg accggcgtgt gatcgaggat atcgaccccg tgtacgtgca atacacgtcc
     4141 ctcgatcgcg gtcaggcgca ggcacgacaa accgatgttg ttggggcgat gcggcgagcg
     4201 ggtcgcgaac aggccccggc tgccgttcgg gtcgcgtggc gatcgcaccg tcatcgagtg
     4261 tccgcggttg aggtggaaca cgaacagaca ccagatgcga tcgaagtcgg cgaggccctc
     4321 gaggccgtcc tcgaggccgc ggtcgggcag cagctcgata cgcgccggca gcatcgcgtc
     4381 gcccttgacc tgggtcgtga tcggcggctg gcgcggcgtg ccgaagcgct cggtgtacgg
     4441 cgagcgcagc acgccgatcg gcgtgaagca aaacggctgg cggtgggcgt cggcgtgctc
     4501 gctggtgtgg tcctgctcgg gtttgcggcg ctgcatcgtc ggcagtgtaa ccccgcggcg
     4561 gcccgcgttc gcgcgcccgc agagcctgcg cggcggttga cgcccaccgg caacgctgtc
     4621 acattggccg cggagggtgg agatgaatta tcgatgggtg gcgggacgtc gcgccgggtc
     4681 ggccgtgggc gtgtttgcgt ggagtttggc ggtcggctgc ggcgattcgg gctcggcggg
     4741 cgagacgacg acgtcgacga gcgcgacgac gacggtcacc agcgcgagcg gcaccggtga
     4801 gccgggcacg accggggacg tcgtcacgac gggcgacacg ccgacgggcg atacgccgac
     4861 gggcggcaac gagggctccg cgaccggcac gaccaccggg gagaccggca cgaccggggt
     4921 cgacagctcg acgggcgaca tcgagggcac gacggcgatc cccgacccgg tgtgcggcga
     4981 cgcggtgctc gacccgggcg agggctgcga cgacggcgag atgaacgcgg acgacggcgc
     5041 ctgtaccatg cagtgtcagc cggccacctg cggcgacggg ctcgtgcagg ccggcgtcga
     5101 gcagtgtgac gagggcgcgg ccaacgcgga cgacggcgcc tgcacctcgc agtgcgtgcc
     5161 ggcgacctgc ggggacctgc tcgtgcagac cggcgtcgag cagtgcgacg acggcaacgc
     5221 cgacgacacc gacgcgtgcg tggcgggctg caagaacgcc gcctgcggcg acggcctggt
     5281 cggccccggc gagctgtgcg acgacggcaa ccagaacgac gacgacagct gcaccaacgc
     5341 gtgcgcctcg cccaactgcg gcgacggcaa gctgcagatg gcgcagggcg aggagtgcga
     5401 cgacggcaac gcgctcgaca gcgatgcgtg cctgagcacc t
//
LOCUS       DASWNJ010000187         2089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_144540, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000187 DASWNJ010000000
VERSION     DASWNJ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144540"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1589)
                     /locus_tag="VGB85_03500"
     CDS             complement(93..1589)
                     /locus_tag="VGB85_03500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0133111.1"
                     /translation="MTTTAPEPRSGPLHADGREVHKGMALLVLAISQLMVVLDATIVN
                     VALPDIARALDVTSQNDLSWIVTGYTLTFGGFLLLGGKLADRLGRRKVFLLGAVLFAV
                     ASLLGGLAGNLGLLIVARLVQGLGGALMSPAALSLLTVVFEEGEERNRALGIWAAITA
                     GGAAVGLILGGVLTEYASWRWVLFVNVPIAVVAVLGTLRFVPESRDERARGFDVPGAL
                     LVTGGLVALVYALVKGNDLGWGSAQTVGTLALSAVLLAAFVAVQRRAPEPLVDFRLFR
                     SRTLLGADLGALFVGAGIFAVFFFMILWMQQINGYSPIKSGFAFLPMTLCIGVAAGIG
                     SQLLGRIGPRPLLIGGPALAALGMLSLGLRLEPGSSYTTVVLPGLVLLALGMGFTFVA
                     LTSAAVAGVPQEDAGVASALLNAGQQVGGALGLAILTAVSTARTEAIGTGAPGTPQFV
                     DALVEGWGAGFLVAAAFLAVAGIIAASLVRVSKEDAAAALKEAGAAAG"
     gene            1673..>2089
                     /locus_tag="VGB85_03505"
     CDS             1673..>2089
                     /locus_tag="VGB85_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0133112.1"
                     /translation="MNPSGEQPTSRDERRRRTEAAILDAARALFAETGYERATIRAVA
                     AAAGVDPALVMQYFGSKEGLFAAAAKWSDDAQTVMTASREQVPAAALHDMIEKFEGAG
                     DREAAVALMRNCLTHPEAARIMRDEVMCDRAAGVART"
BASE COUNT          334 a          724 c          804 g          227 t
ORIGIN      
        1 acaaccgatc agctcgcgcg tccgaccgct cgcgggtcga tcggttgtgg ggagtggcgg
       61 ccgtccgtac gcgggcggga tgccacagcc gatcagccgg cggcggcgcc ggcctctttc
      121 agggccgcgg cagcgtcctc cttggacacg cggaccaggc ttgcggcgat gatgccggcg
      181 acggcgagga aggccgccgc gacgaggaag ccggcacccc agccctcgac gagcgcgtcc
      241 acgaactgcg gcgtgcccgg cgcaccggtg ccgatcgcct cggtgcgggc ggtgctgacg
      301 gccgtgagga tcgccaggcc gagcgcgccg ccgacctgct ggccggcgtt gagcagggcg
      361 ctggcgacac cggcgtcctc ctgcgggacg cccgccacgg cggcgctggt gagcgcgacg
      421 aaggtgaagc ccatgcccag ggccagcagg accaggccgg gcagcaccac cgtcgtgtac
      481 gaggagccgg gctccaggcg caggccgagc gagagcatgc cgagcgccgc gagcgccggg
      541 ccgccgatga gcagcggccg cgggccgatc cggccgagca actgcgagcc gatgccggcg
      601 gcgacgccga tgcacagcgt catcggcagg aaggcgaagc cgctcttgat cggcgagtag
      661 ccgttgatct gctgcatcca caggatcatg aagaagaaga ccgcgaagat gccggcgccg
      721 acgaacagcg ccccgaggtc ggcgccgagc agcgtccggg agcggaacag ccggaagtcg
      781 acgagcggct cgggcgcgcg gcgctggacg gcgacgaagg ccgcgagcag caccgcggac
      841 agcgcgagcg tgccgaccgt ctgcgccgag ccccagccga ggtcgttgcc cttgacgagc
      901 gcgtagacca gggccaccag cccgccggtg acgagcaggg cgcccggcac gtcgaagccg
      961 cgggcccgct cgtcgcggct ctccggcacg aagcgcagcg tgccgaggac ggccacgacc
     1021 gcgatcggga cgttgacgaa cagcacccag cgccagctgg cgtactcggt cagcacgccg
     1081 ccgaggatga ggccgacggc ggcgccgccg gcggtgatgg ccgcccagat gccgagcgcg
     1141 cggttgcgct cctcgccctc ctcgaagacg acggtgagca gcgagagcgc ggcgggggac
     1201 atcagcgcgc cgcccaggcc ctgcaccagg cgggcgacga tgagcagccc gaggttgccg
     1261 gcgaggccgc ccagcagcga cgcgacggcg aacagcaccg cgccgagcag gaacaccttg
     1321 cgccggccca gccggtcggc gagcttgccg ccgagcagca ggaagccgcc gaaggtgagc
     1381 gtgtagccgg tcacgatcca cgagaggtcg ttctggctgg tgacgtcgag ggcgcgggcg
     1441 atgtcgggga gggcgacgtt cacgatcgtc gcgtcgagca cgaccatgag ctggctgatc
     1501 gcgagcacga gcagggccat gcccttgtgc acctcgcgac cgtcggcgtg gaggggtccc
     1561 gaccggggtt caggggcggt ggtggtcacg aggcggtgct ccttctgcgg ggttggctca
     1621 acgaccgtag accgttgatg cgtccacggt caacggctgt agagtggcga ccgtgaaccc
     1681 ctccggtgaa cagccgacct cgcgggacga acgccgcagg aggaccgagg cggcgatcct
     1741 cgacgcggcc cgggcgctgt tcgccgagac cggctacgag cgggcgacca tccgcgcagt
     1801 ggccgccgct gccggcgtcg acccggccct cgtcatgcag tacttcggca gcaaggaggg
     1861 cctgttcgcc gctgcggcga agtggtccga cgacgcgcag acggtcatga ccgcctcgcg
     1921 ggagcaggtg cccgctgccg ccctgcacga catgatcgag aagttcgagg gcgccggcga
     1981 ccgcgaggcg gccgtcgcgc tgatgcggaa ctgcctcacg caccccgagg cggcccggat
     2041 catgcgcgac gaggtcatgt gcgaccgcgc tgccggtgtc gcgcgcacg
//
LOCUS       DASWNJ010000188         2337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_639988, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000188 DASWNJ010000000
VERSION     DASWNJ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639988"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VGB85_03510"
     CDS             complement(<1..180)
                     /locus_tag="VGB85_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133113.1"
                     /translation="MRQVQKVRSWAAALVVLGSASAAASPVRLGTGSKGGDYDLLGQA
                     LVADLKAQGRDLELAP"
     gene            complement(177..914)
                     /locus_tag="VGB85_03515"
     CDS             complement(177..914)
                     /locus_tag="VGB85_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133114.1"
                     /translation="MVASTQGDALGRLVVVNWAADALGLVRALQELGANDLVLVGTSP
                     AAEVEQCLAGVRERGALRYLRASVGDGVEVFAEFLAREVEVGAAQSVIVLPHRGRGET
                     DAFSRLTCTAIHRACRGQPPRVVVAVEDPEATHEFAGLGVATIFYPGFLRAALLAHAC
                     VDLGVLRFMFALLRGKLRVQSWPLPAGLQGRSFRDACMTVETDPEGRPVTVLGVFTPD
                     AGMLVNPGPAAPLAAVTALLVLTGQDA"
     gene            1106..1900
                     /locus_tag="VGB85_03520"
     CDS             1106..1900
                     /locus_tag="VGB85_03520"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY0133115.1"
                     /translation="MTAETSALLFLGLIAAVSLLLGYIGATVGLVLGQLRVALLIYAL
                     GDAAVGTATSLAISTAGTLIGTLRHAVGGRVQLALLLTVGAPSAAAAFFTARYAHLVD
                     ARLLKLAIAGILIVTGGSMLVRREPKPDEQAAAPKVPPMLAEVAVGTVLGAISGLVGL
                     LLGSLRLPAMIRYARVPTAIAIGTNMAIGTLTGLSAGVATFLEGQVDLRAFAVVAPAT
                     VIGAHLGAGKTGKLAPETLRAVIAWVLVATGLLMLGEGLIRKIFAP"
     gene            1897..>2337
                     /locus_tag="VGB85_03525"
     CDS             1897..>2337
                     /locus_tag="VGB85_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133116.1"
                     /translation="MNCSLSCSPVLADSPRRTGRDAATFFAWTCAALPRVFPGPALAP
                     AELRAAWTLAARDIAARLALACAREGLVESSWTDRLQLHTPRGPLGLPIARRGAFELH
                     RPDLDAAREPALEHPGLLLDALAPALALAPATAARIDHELADSVF"
BASE COUNT          290 a          917 c          813 g          317 t
ORIGIN      
        1 cggcgcgagc tcgaggtcgc ggccctgagc cttcaagtcg gcgacgagcg cctggccgag
       61 caggtcgtag tcgccgccct tgctgccggt gccgaggcgc accgggctcg cggcggcgct
      121 cgccgacccc aggaccacca gcgcggccgc ccacgacctg accttttgga cctgtctcat
      181 gcgtcctgtc cggtcagcac gagcagcgcg gtcacggcgg cgagcggcgc cgcggggcca
      241 ggattgacga gcatgccggc gtccggggtg aacacgccga gcacggtgac ggggcggccc
      301 tcggggtcgg tctcgacggt catgcaggcg tcacgaaagc tgcgcccctg cagcccggcc
      361 ggcagcggcc agctctgcac gcgcagtttg ccgcgcagca gcgcgaacat gaagcgcagc
      421 accccgaggt cgacgcacgc gtgcgcgagc agtgcggcgc gcaaaaagcc cggatagaag
      481 atcgtcgcca cgccgaggcc ggcgaactcg tgggtggcct ccgggtcctc gaccgcgacg
      541 acgacccgcg gcggctgtcc gcgacaggcc cggtggatcg ccgtgcacgt caggcgggag
      601 aacgcgtccg tctcgccgcg gccgcggtgc ggcagcacga tcaccgattg cgccgcgccg
      661 acctcgacct cgcgcgccag aaactccgcg aacacctcga cgccgtcgcc gaccgaggcc
      721 cggaggtaac gcagcgcccc gcgctcccgg acccccgcga ggcactgctc gacctccgcg
      781 gcgggcgacg tgccgacgag cacgaggtcg ttcgcgccga gctcttgcag ggcgcgcacc
      841 aggcccagcg cgtccgcggc ccagttcacc accaccagcc gaccgagggc gtcgccctgg
      901 gttgatgcga ccaccgccat cgagagcgcg acgatgccgc ccggcccggt ccggtgtcaa
      961 gccggccgtg cgcgatcggt gacgcgtgct aatgtcgacg cgtgccgcac gagccggacg
     1021 tgtcccggga cgcaccgcga ctgccctttc gggcggctgg actcacttcc gtggcagttg
     1081 gctggagcgg aaagctcgag gccacgtgac cgccgagacc tccgcgctgc tgttcctcgg
     1141 gctgatcgcg gcggtgtcgc tgctgctcgg gtacatcggc gcgaccgtcg gcctcgtgct
     1201 cggtcagctg cgcgtcgccc tgctgatcta cgcgctcggt gacgccgccg tcggcaccgc
     1261 gaccagcctg gcgatctcga ccgcgggcac gctgatcggc acgctgcgcc acgcggtcgg
     1321 cggtcgcgtg cagctggccc tgctgctgac cgtcggcgcc ccctcggccg ccgccgcgtt
     1381 cttcaccgcc cgctacgccc acctcgtcga cgcgcgcctg ctcaagctgg cgatcgccgg
     1441 catcctgatc gtcaccggtg gttcgatgct cgtccgccgc gagccgaagc ccgacgagca
     1501 ggccgctgcc ccgaaggtgc cgccgatgct cgccgaggtc gcggtcggca ccgtgctcgg
     1561 cgcgatctcg gggctcgtcg gcctgctgct ggggtcgctg cgcctgccgg cgatgatccg
     1621 ctacgcccgc gtgccgaccg cgatcgcgat cggcaccaac atggcgatcg gcacgctgac
     1681 cgggctgtcg gcgggcgtcg caaccttcct cgagggacag gtggaccttc gcgccttcgc
     1741 ggtcgtcgca ccggccacgg tgatcggcgc ccacctcggc gccggcaaga ccggcaagct
     1801 cgcccccgag accctgcggg ctgtcatcgc ctgggtgctc gtcgccaccg gcctgttgat
     1861 gctcggtgag ggcctgatca ggaagatctt cgcgccatga attgcagcct ctcatgttca
     1921 cctgtcctcg cggacagccc ccgacgcacc ggccgcgacg ccgccacctt cttcgcgtgg
     1981 acctgcgccg cgctgccgcg cgtgttcccc gggcccgcgc tggcgccggc cgagctgcgc
     2041 gccgcgtgga ccctcgccgc ccgcgacatc gcggcccgcc tcgcgctcgc ctgcgcccgc
     2101 gaggggctcg tcgagtcgag ctggaccgac cgcttgcaac tgcacacgcc gcgcggcccg
     2161 ctcggcctcc cgatcgcgcg tcgcggcgcc ttcgagctgc accgccccga cctcgacgct
     2221 gcgcgtgaac cggcgctcga gcacccgggc ctgctgctcg acgcgctcgc gccggcgctc
     2281 gcgctcgcgc cggcgaccgc ggcccgcatc gatcacgagc tggccgacag cgtgttc
//
LOCUS       DASWNJ010000189         2213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_640008, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000189 DASWNJ010000000
VERSION     DASWNJ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640008"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VGB85_03530"
     CDS             <1..190
                     /locus_tag="VGB85_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133117.1"
                     /translation="CKDGGGGSNSPESVEGSADCPRSRPRNGASCPRGATDFCVYRSG
                     KGDHVCACGKGNWRCAAK"
     gene            233..856
                     /locus_tag="VGB85_03535"
     CDS             233..856
                     /locus_tag="VGB85_03535"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0133118.1"
                     /translation="MRWYDWFASFYDRSLEPLYRDARAAAVTALAAEPGQTVLDLPCG
                     TGQSFDGLAAAVGPGGRIIGVDLSEGMLRVAEARARRGGHRQIALRQRDVHLLDAIDL
                     APAGRDFQLDRLHIFLGLTAFPRWEAAFERLWGLLAPGGRCVIVDVHAAQLGLQGRLV
                     NLIARADLRREVWRPLERRADNYEYAELTRVHAHGGQIFVASGDKPR"
     gene            complement(860..1372)
                     /locus_tag="VGB85_03540"
     CDS             complement(860..1372)
                     /locus_tag="VGB85_03540"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY0133119.1"
                     /translation="MSQSWFKRRSNDLVTVVEADLLPVLNIMFLLIPALLLAMEFASM
                     ATIEVAPPRMCASCDSKPATEDPFKLTIEIRSDGFQAEVNGSASGEPIPLRDGAHDFA
                     ALAAAAKQLKFAHPDTSDVTVRAESAVRLQTLVETIDTLRGEDCKLAGMRSGEAAPSA
                     CMFWQTQVAS"
     gene            complement(1539..>2213)
                     /locus_tag="VGB85_03545"
     CDS             complement(1539..>2213)
                     /locus_tag="VGB85_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133120.1"
                     /translation="PVGMTSSTEPVVTTGGPMVCPDPPVNTQLVVEVTRNGAPYQDCV
                     LSEDQELEGLLFMTGTSMSLRLAPDCMPGDPQDELVLASGLDLPALDPVCVRVTIRWD
                     GEGVHCKLGLLQVHDAETDKILYVGAFRLDPPDDFPLHTNGFNVTSCGCPEDEPECCE
                     PRPGILDLAPSNGKPVAQGEHGYAKLKGVEYDFYNLQSWVDPTCVLGDDHGRHIDWLA
                     VVVEGG"
BASE COUNT          313 a          857 c          730 g          313 t
ORIGIN      
        1 ctgcaaggac ggcggcggcg gcagcaacag ccccgagagc gtcgagggct cggccgactg
       61 tccgcgctcg cgcccgcgaa acggcgcctc gtgcccgcgc ggcgcgaccg acttctgcgt
      121 ctaccgctcg ggcaagggcg accacgtgtg cgcgtgcggc aagggcaact ggcgctgcgc
      181 cgccaagtga ccgcttgccc gcggccccgc gccgcggata cgctcccgcg cgatgcgctg
      241 gtacgactgg ttcgcctcgt tctatgaccg cagcctcgag ccgctgtatc gcgacgcccg
      301 cgccgcggcc gtcacggccc tcgccgccga gccgggccag acggtgctcg acctgccctg
      361 cggcaccggc cagagcttcg acggcctcgc cgcggccgtc ggtccgggcg gccgcatcat
      421 cggcgtcgac ctcagcgagg gcatgctgcg cgtcgccgag gcgcgcgctc gacgaggcgg
      481 ccaccgccag atcgccctgc gccagcgcga cgtccacctg ctcgacgcga tcgacctcgc
      541 ccccgccggc cgcgacttcc agctcgaccg cctgcacatc ttcctcggcc tgaccgcctt
      601 cccgcgctgg gaggccgcct tcgagcgcct gtggggcttg ctcgcgccgg gcggtcgctg
      661 cgtcatcgtc gacgtgcacg ccgcccagct cggcctccag ggccgcctgg tcaacctgat
      721 cgcccgcgcc gacctgcgcc gcgaggtctg gcgcccgctc gagcgccgcg ccgacaacta
      781 cgagtacgcc gagctgaccc gcgtgcacgc ccacggcggc cagatcttcg tcgccagcgg
      841 cgacaagccg cgctgacgat cagctggcga cctgggtctg ccagaacatg caggccgacg
      901 gggccgcctc gccgctgcgc atcccggcga gcttgcagtc ctcgccgcgc agcgtgtcga
      961 tcgtctcgac gagcgtctgc aggcgcaccg ccgactcggc gcggaccgtc acgtcggagg
     1021 tgtccgggtg cgcgaacttc agctgcttcg cggcggccgc gagcgcggcg aagtcgtggg
     1081 ccccgtcgcg cagcgggatc ggctcgccgc tggcgctacc attgacctcc gcctggaacc
     1141 cgtcgctgcg gatctcgatc gtcagcttga acggatcctc cgtcgccggc ttgctgtcac
     1201 acgacgcaca catccgcggc ggcgcgacct cgatcgtcgc catgctcgcg aactccatcg
     1261 ccagcaacag cgccgggatc agcaggaaca tgatgttgag gacaggcaac aggtccgcct
     1321 cgaccacggt gaccaggtcg ttcgaacgcc tcttgaacca gctctgtgac atgacgccct
     1381 ccgctgcacg acgcagcacg acaggaccag cgcccgcgcc agcaaccagc gcgcagctcc
     1441 ccgtcggcga gcaagacagc cgcgagcccg cgaggttccc cgcggcccgc gaaaatcttc
     1501 acgagccgcc gcgcgcacgg ccgcgccccg gctcgcgctc acccgccctc gaccaccacc
     1561 gccagccagt cgatatgccg gccgtggtca tcccccagca cgcaggtcgg gtcgacccac
     1621 gactgaagat tataaaagtc gtactcgacg cccttgagct tcgcgtagcc gtgctcgcct
     1681 tgcgcgactg gcttgccgtt gctcggggcg aggtcgagga tgccaggccg cggttcgcag
     1741 cactccggct cgtcctccgg gcacccgcag ctggtcacgt tgaagccgtt ggtgtgcagc
     1801 ggaaagtcgt cgggcgggtc gaggcgaaac gccccgacgt acaggatctt gtcggtctcc
     1861 gcgtcgtgga cttgcagcag gccgagcttg cagtgcacgc cctccccgtc ccagcggatc
     1921 gtgaccctca cgcacaccgg gtcgagcgcc ggcaggtcga ggcccgacgc gagcaccagc
     1981 tcgtcctgcg ggtcgccggg catgcagtcc ggggccagcc tcagcgacat gctcgtgccc
     2041 gtcatgaaca gcagaccctc gagctcctgg tcctcggaga gcacacaatc ctggtagggc
     2101 gcgccgttgc gcgtcacctc gacgacgagc tgcgtgttga cgggcgggtc ggggcatacc
     2161 atcggcccgc cggtcgtcac gaccggctcc gtgctgctcg tcatccccac ggg
//
LOCUS       DASWNJ010000190         2915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_286118, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000190 DASWNJ010000000
VERSION     DASWNJ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286118"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1110)
                     /locus_tag="VGB85_03550"
     CDS             complement(7..1110)
                     /locus_tag="VGB85_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133121.1"
                     /translation="MPSARLAAAVCLLGLACNGPGDSSTGFTTPGVTTRPSDTNSGES
                     TSTGDGTSSTSSTSSTSSTSTGSGSADGSTGSSTTGAVFPDMGMMPDFGGPPMGCKGK
                     IDFLFVISRDGSMSGAQNQIVAATPAFLATIQAQFDDFDVHIMVVDSEQKWWVDYCED
                     QCPGPCKNAPGYPCGAVPSACDTTVGAGTVYNVGPYATNMPCDLGGRRYITKDTPDMA
                     SAFECIARVGVSGNNLMGDALVAAVGPQLNASQPEPGGCNAGFLRDDALLVLTMIGPE
                     DDPPGGSQGKWQEWRQAVVDAKHGDPNAIVALGIISGPDCKPNIDPALRLCNLIPSFP
                     NNVFEHLTLEDYGDAFDKAALLALDACSVFVPG"
     gene            complement(1155..1607)
                     /locus_tag="VGB85_03555"
     CDS             complement(1155..1607)
                     /locus_tag="VGB85_03555"
                     /inference="COORDINATES: protein motif:HMM:NF025444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4259 domain-containing protein"
                     /protein_id="HEY0133122.1"
                     /translation="MGAWGHESFANDSALDWLSALVRGGPALLAEALDRVGSAAADEY
                     LEVDESSAALAAAELVAAALGGGEDRLPQEALAWLKDHVHAARAIGAERARRAVERVF
                     RDSELRELWEENGEQTEWHAEVRELLERLVAGSRPLGPGADLDLEGAQ"
     gene            complement(1609..2136)
                     /locus_tag="VGB85_03560"
     CDS             complement(1609..2136)
                     /locus_tag="VGB85_03560"
                     /inference="COORDINATES: protein motif:HMM:NF026766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 26/phosphotriesterase HocA family
                     protein"
                     /protein_id="HEY0133123.1"
                     /translation="MTGGKRTTRIRDKVGNVLAVPLGEDRFGFAWVLNSPLVAFFDRR
                     CRADDFSPVDEIVRSPIAFTIWVMKNAVTSGKWPCLGHMPVPEHLLEPRWFFNQDSIN
                     GRIHKTLTGAEEVPAMADEVRRLECAAVWSDMHVEERLRAHFEGRPSIFAESMKVKPP
                     EFFEAKGNTIAARRP"
     gene            complement(2133..>2915)
                     /locus_tag="VGB85_03565"
     CDS             complement(2133..>2915)
                     /locus_tag="VGB85_03565"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HEY0133124.1"
                     /translation="HYLPALGRWTAPDPYYVLNPGANLERPGERNLYGYAGNNPVQHI
                     DSTGHSWASWIVKGTKAGLKWFYKGYDKVDEFSGIVDDAATIVSAEAGIGSRVLSVLS
                     LASEILPVSAGDLKDGYRWFRGGEEVLDAAASAKRAERREVSNAIQNSDEITHQTYLR
                     TNPATGQVYSGRTHGTGTPAQNIRKRESSHHMNEQGYGPAVLDKSSKNGDAIRGREQF
                     NIEQHGGAQRSGGTSGNRINGISPRNPKGPRYRAAAKQEFGE"
BASE COUNT          451 a          941 c          980 g          543 t
ORIGIN      
        1 cccctactag ccgggcacga acacgctgca agcgtccagg gcgagcagcg ccgccttatc
       61 aaaggcatcc ccgtagtcct cgagggtcag gtgctcgaag acgttgttgg ggaacgacgg
      121 gatgaggttg cagagtcgta aggcgggatc gatgttcggc ttgcagtcgg ggccgctgat
      181 gatgccgagg gcgacgatgg cgttaggatc gccgtgcttg gcgtccacga ccgcttgccg
      241 ccactcctgc cacttccctt gggacccgcc gggtgggtcg tcctcgggcc cgatcatcgt
      301 cagcacgagg agcgcatcgt cccgcagaaa gcccgcgttg catccgcccg gctcgggctg
      361 ggaggcgttg agctgggggc ccacggcggc gaccagtgcg tcacccataa ggttgttgcc
      421 cgagacgccg acgcgggcga tgcactcgaa ggccgaggcc atgtcggggg tgtccttggt
      481 gatgtatcgg cgcccgccga ggtcgcaggg catgttcgtc gcgtatggcc cgacattgta
      541 gacggtcccc gcgccgacgg tcgtgtcgca ggccgatgga acagcgccgc acgggtagcc
      601 cggtgcgttt ttgcacggac caggacactg gtcctcgcag tagtcgaccc accacttctg
      661 ttcgctatcg acgaccatga tgtggacgtc gaagtcgtcg aactgggcct ggatggtggc
      721 gaggaaggcc ggagtcgcgg cgacgatctg gttctgcgcc ccggacatgc tgccgtctcg
      781 agagatgacg aagagaaagt cgatctttcc cttgcagccc atcggcgggc cgccgaagtc
      841 gggcatcatg cccatgtccg ggaacacggc gcctgtcgtc gatgagcccg tgctgccgtc
      901 cgccgagccg ctcccggtcg aggtgctcga ggtgctcgag gtgctcgagg tgctcgaggt
      961 gccatcgcca gtcgaggtcg actcgcccga gttggtgtcg ctcgggcgcg tggtcacgcc
     1021 tggcgtggtg aagcccgttg acgagtcgcc ggggccgttg caggcgagcc cgaggaggca
     1081 cacagcggcg gcgaggcgtg ccgagggcat aggggtgcag tctagcgcgg agccgcgccg
     1141 atgccaaacg tggctcattg agcgccctcc aggtctagat cggcccctgg gccaagcggc
     1201 cgtgatccgg caaccaggcg ctcgaggagc tcacggacct ctgcgtgcca ctccgtttgc
     1261 tcgccgtttt cctcccagag ctcgcggagt tccgaatccc gaaacacccg ctccacggcc
     1321 ctccgcgctc gctcggcacc gatagcccgc gcagcatgaa catggtcttt cagccaggcg
     1381 agtgcttcct gcgggagccg gtcctcgccg cccccgagcg ccgcggccac gagctcggcg
     1441 gctgccaagg cggccgagct ctcatccacc tccaggtact cgtcggctgc cgcagagcca
     1501 acgcgatcta gcgcctcggc gagcagtgcg ggcccgccac ggacgagcgc agacagccag
     1561 tccaaggccg agtcgttggc gaaactctcg tgtccccacg cgcccatgtc agggtctcct
     1621 cgctgcgatt gtgtttccct ttgcctcgaa aaactcaggc ggtttgacct tcatcgactc
     1681 ggcgaagata ctgggtcgcc cttcaaagtg ggcccgcagt cgctcctcaa catgcatgtc
     1741 gctccagacg gccgcacact cgagccgcct tacctcgtcg gccatggccg ggacttcctc
     1801 ggcgcccgtc aaggtcttgt ggatcctgcc gttgattgag tcctggttga agaaccatcg
     1861 tggctcaagt aagtgctcgg ggacgggcat atggcccagg caaggccatt taccgctggt
     1921 tacggcgttc ttcatgaccc agattgtgaa tgcgatcggg ctccgcacga tctcgtcaac
     1981 aggcgagaaa tcatcagccc ggcatcggcg gtcgaagaac gcaaccagcg gcgagttgag
     2041 gacccaagcg aagccgaacc ggtcctcacc aagagggacc gcgaggacat tcccgacctt
     2101 gtcgcggatc ctggtcgtgc gctttcctcc cgtcattccc cgaactcctg cttcgccgcg
     2161 gccctgtacc tcggtccttt gggattgcgg ggggagatcc cgttgatcct gtttcccgaa
     2221 gtgccgcccg atctctgggc gccgccatgc tgctctatgt tgaactgctc gcgaccgcgg
     2281 atggcgtctc cgttctttga ggacttgtcc aggacggcag ggccgtagcc ttgttcgttc
     2341 atgtggtgac tcgactcgcg cttgcggatg ttctgcgctg gggtgcccgt gccatgcgtg
     2401 cgcccggagt atacctggcc ggtagccggg ttcgtgcgga ggtacgtctg gtgcgtgatc
     2461 tcgtcgctgt tctggatcgc attagagacc tccctgcgct cagcgcgctt ggcggacgcg
     2521 gcggcgtcca ggacctcctc gccgccgcgg aaccagcggt agccgtcttt gaggtcgcca
     2581 gcgctgaccg gaaggatctc gctggcgagg gacaggacag acagcacgcg gctgccgatg
     2641 cccgcttcgg cgctgacgat cgtcgcggcg tcgtcgacga tgccgctgaa ctcatcaacc
     2701 ttgtcgtagc ccttgtagaa ccacttgagg ccggctttag tgcccttgac gatccacgag
     2761 gcccaactgt ggccggttga gtcgatgtgc tggacggggt tgttgcccgc gtagccgtag
     2821 agattgcgct cgccggggcg ctcgaggttc gcgccggggt tgaggacgta gtacgggtcc
     2881 ggcgcggtcc agcggcccag cgcgggcagg tagtg
//
LOCUS       DASWNJ010000191         1589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_604066, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000191 DASWNJ010000000
VERSION     DASWNJ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604066"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VGB85_03570"
     CDS             <1..381
                     /locus_tag="VGB85_03570"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEY0133125.1"
                     /translation="GVSVERLDRMHTALLDNPVSLDQPVGGGNSDGSGLSLADALTDP
                     DAELPADLLDQAHLLSQLRESLAALPAMEADILRKRMGLDDEEALTLKEIGDQYSLSR
                     ERIRQLQEVALNHLRAEFRRRALL"
     gene            complement(378..968)
                     /locus_tag="VGB85_03575"
     CDS             complement(378..968)
                     /locus_tag="VGB85_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133126.1"
                     /translation="MTDEPRSLVRVEAARLPDAYEEKFMAGEGTVLYRDKRRAPWPAH
                     ALLGAAALAVIAVSVASGELLALAIALPVLAMIWVLFAVLRVTVSAGVVTVQYGIFGP
                     EIPVAAIELCEATTYDWKRFGGWGIRRGRGGEWIYNMPGDGGHAVRIVWRDRKGRRRV
                     TLIGSRHADQLASTIRGARAALPAAPGRAALPDGED"
     gene            1084..>1589
                     /locus_tag="VGB85_03580"
     CDS             1084..>1589
                     /locus_tag="VGB85_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133127.1"
                     /translation="MGVSKGSDHYVGHSDQSSLGDALQDAVNQALADQEGADRMIDFE
                     VTKIRGRAGGIAGSRDIWVEIAVGDGQQPGKGHDDNKEEPGKYTTLAVGEEGGGLGDH
                     PGHDLGPGMSTRAMGEEGGLAAEAPASGDEQVFTTMAVGEEGGELAADAPASGDEQVF
                     TTMAVGEEG"
BASE COUNT          243 a          620 c          524 g          202 t
ORIGIN      
        1 ggcgtcagcg tcgagcgcct cgaccgcatg cacaccgccc tgctcgacaa ccccgtcagc
       61 ctcgaccagc cggtcggcgg cggcaactcg gacggctctg gcctcagcct cgccgacgcg
      121 ctgaccgacc ccgacgccga gctgcccgcg gacctgctcg accaggccca cctgctcagc
      181 cagctgcgcg agtccctggc cgcgctgccg gcgatggagg ccgacatctt gcgcaagcgc
      241 atgggcctcg acgacgagga ggcgctgacg ctcaaggaga tcggcgacca gtactcgctg
      301 tcacgcgagc gcatccgcca gctgcaggag gtcgcgctca atcacctgcg cgccgagttc
      361 cgccgccgcg ccctgctcta atcctcgccg tccggcagcg ccgcgcgccc gggcgccgcc
      421 ggcagggccg cgcgtgcccc cctgatcgtg ctggcgagct ggtcggcgtg acggctgccg
      481 atcagcgtca cgcgccgccg gcccttgcgg tcgcgccaca cgatgcgcac cgcgtggccg
      541 ccgtcgcccg gcatgttgta gatccactcg cccccgcgcc cgcgccggat cccccagccg
      601 ccgaaccgct tccagtcgta ggtggtcgcc tcgcacagct cgatcgcagc cacggggatc
      661 tccgggccga agatcccgta ctgcaccgtc accacgccgg ccgacaccgt cacgcgcagc
      721 accgcgaaca gcacccagat catcgccagg accggcagcg cgatcgccag cgccagcagc
      781 tcaccgctgg cgacgctgac cgcgatcacg gcgagcgcgg ccgccccgag cagcgcgtgc
      841 gccggccacg gcgcgcgccg cttgtcgcgg tacagcaccg tgccctcgcc ggccatgaac
      901 ttctcctcgt acgcgtccgg caggcgcgcc gcctcgaccc gcacgagcga cctcggttcg
      961 tcggtcatcg actcgagcgt aagtccgccc cctggtgcgg tcaatcgcgc gcgaacccgc
     1021 gtctccttga caagcatcga ccgtccgcgt ctcctgctcc tcgatccgca ggaggaggca
     1081 gccatgggag tcagcaaggg aagcgatcac tatgtcggtc attcggacca atcttcgctc
     1141 ggcgacgcgt tgcaggacgc cgtcaatcag gccctcgccg atcaggaggg cgccgatcgc
     1201 atgatcgact tcgaggtcac caagatccgc ggccgcgccg gtggcatcgc cggctcgcgt
     1261 gacatctggg tcgagatcgc cgtcggcgac ggccagcagc ccggcaaggg ccatgacgat
     1321 aataaagagg agccgggcaa gtacaccacc ctcgccgtcg gcgaggaggg cggcggcctc
     1381 ggagaccacc ccggccatga cctcggcccc ggcatgagca cccgcgcgat gggtgaggag
     1441 ggcggcctcg ctgccgaggc acccgcgtcc ggcgacgagc aggtcttcac caccatggcc
     1501 gtcggcgagg agggcggcga gctcgctgcc gatgcgcccg cgtccggcga cgagcaggtc
     1561 ttcaccacca tggccgtcgg cgaggaggg
//
LOCUS       DASWNJ010000192         2725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_568890, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000192 DASWNJ010000000
VERSION     DASWNJ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568890"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..1948)
                     /locus_tag="VGB85_03585"
     CDS             complement(122..1948)
                     /locus_tag="VGB85_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HEY0133128.1"
                     /translation="MTTASLELAPAEVEVTYHADGSMVLRSPHPLGPYPATLGDRLRF
                     WAETTPHRPFLGERAGAGWRVVTHGEAHATARRLGAALLARGLGPRRPLVILSDNSVD
                     NALLQLAAMQVGIPAVPVSPAYSLMSQDLANLGHVVERVRPGLVYAADGQRFARALAS
                     VARPGLFTVVSHMPGHEHELLSDLLEETHDLATADEAHANTGPDTVAKILFTSGSTGL
                     PKGVLNTQRMLCSNQQAIAQSWPFLRARPPVLVDWLPWHHTFGGNHNFNMALYHGGAL
                     YIDDGKPAPGLIDRSAENLRAHPPTLYFNVPRGFDVLLPLLEDDASLREALFGRLDLV
                     FYAGAALPQHLWARLEACALRARPTPVFMASAWGATETSPLITTVHFPIAHAGIIGLP
                     APGCEVKLVPHEGKLEMRARGPNITPGYVREPEQTAAAFDEQGWYRTGDAGRLADPAD
                     PRKGIVFDGRVAEDFKLTSGTWVNVGALRIAVLAAGAATMQDAVVAGEGQAEIGLLVF
                     PSVHGCSALAPDCEGLDALVDDPRVRAAVLAALERHNAEHDGSSRRIGRCLLLREPPA
                     LDAGEITDKGYINQRAVLRRRAEALARLYAEPPDASVLVLGR"
     gene            complement(1975..>2725)
                     /locus_tag="VGB85_03590"
     CDS             complement(1975..>2725)
                     /locus_tag="VGB85_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133129.1"
                     /translation="PAPAPPAKPHGRVFLAASESGLIPIACHEAHVPKFSTGEPCLAL
                     APVGTELRLESGALAKVTGTGPAACGGGQALVVEAPAEALRGHATAPSDPSFVETVPP
                     STPAEAAEAAPAGTLASIAAVVATDYPALGDTKGLQLQQWSEIDLDGDRKPEGLASVT
                     LPATGGEDDAPPAFAGLYLVPAVEGARPRKIKGEPGAGVQYTVLGVVDLDADGRPELW
                     LNSYDDDGFAWSIEQPGSAQLTELGRWRCDA"
BASE COUNT          365 a          990 c         1015 g          355 t
ORIGIN      
        1 gagccgatcg acagcaaccg caccccggcc ggccgcgcca agaaccgccg catcgagttc
       61 gagctgatcg tcgaccgcgt gcagtgacgg tcacgcaccc gctcgcctcg cagcgaaccc
      121 tctagcgccc gagcaccagc accgacgcgt cgggtggctc ggcgtagagg cgcgcgaggg
      181 cctcggcgcg gcggcggagc accgcgcgct gattgatgta acccttgtcc gtgatctcgc
      241 cggcgtcgag cgcgggcggc tcgcgcaaca gcaggcagcg gccgatgcgg cggctgctgc
      301 cgtcgtgctc ggcgttgtgg cgctcgagcg cggcgagcac cgcggcgcgc acgcgcgggt
      361 cgtcgacgag cgcgtcgagc ccctcgcagt ccggcgcgag cgcgctgcag ccatgcacgc
      421 tcgggaacac gagcagcccg atctccgcct gaccttcacc ggcgaccacc gcgtcctgca
      481 tcgtcgccgc gccggcggcg agcaccgcga tgcgcagggc gccgacgttc acccaggtgc
      541 ccgaggtcag cttgaagtcc tcggcgacgc ggccgtcgaa cacgatgccc ttgcgcgggt
      601 cggcggggtc ggcgaggcga ccggcgtcgc cggtgcgata ccagccctgc tcatcgaacg
      661 cggccgccgt ctgttcgggc tcgcggacgt agccgggcgt gatgttgggg ccacgcgcgc
      721 gcatctcgag cttgccctcg tgcgggacca gcttgacctc gcagccgggc gcgggcagcc
      781 cgatgatgcc ggcgtgcgcg atcgggaagt gcaccgtcgt gatcagcggc gaggtctcgg
      841 tcgcgcccca ggccgaggcc atgaacaccg gcgtcggccg ggcccgcagc gcacaggcct
      901 cgaggcgggc ccacaggtgc tgcggcagcg cggcgccggc gtagaacacc aggtcgaggc
      961 gtccgaacag cgcctcgcgc agcgacgcgt cgtcctcgag cagcggcagc agcacgtcga
     1021 agccgcgcgg cacgttgaag tacagcgtcg gcgggtgcgc gcgcaggttc tcggcgctgc
     1081 gatcgatcag ccccggcgcc ggcttgccgt cgtcgatgta gagcgcgccg ccgtggtaga
     1141 gcgccatatt gaagttgtgg ttgccgccga aggtgtggtg ccacggcagc cagtcgacga
     1201 gcaccggcgg gcgcgcgcgc aggaacggcc acgactgcgc gatcgcctgt tggttactgc
     1261 acagcatgcg ctgcgtgttg agcacgccct tgggcagccc ggtcgacccc gaggtgaaca
     1321 ggatcttcgc caccgtgtcg ggcccggtgt tcgcgtgggc ctcgtcggcg gtggcgaggt
     1381 catgtgtctc ttcgagcagg tccgagagca gctcgtgctc atgcccgggc atatgactga
     1441 cgaccgtgaa cagccccggc cgcgccaccg acgcgagcgc gcgagcgaag cgctggccgt
     1501 cggccgcgta taccaggccc ggcctgacgc gctcgacgac gtggccgagg ttggcgaggt
     1561 cctgcgacat caggctgtac gccggcgaca ccggcaccgc cgggatcccg acctgcatcg
     1621 ccgccagctg caacaatgcg ttgtcgacgc tgttgtcgga caggatcacc agcggacggc
     1681 gcgggccgag accgcgcgcc agcagggccg caccgaggcg gcgcgccgtc gcgtgggcct
     1741 cgccgtgcgt gacgacccgc cagcccgccc ccgcgcgctc gcccaggaac ggccgatgcg
     1801 gcgtcgtctc ggcccaaaag cgcaggcggt cgccgagcgt cgccgggtac ggcccgagcg
     1861 ggtggggcga tcgcagcacc atgctgccgt cggcgtgata ggtgacctcg acctcggccg
     1921 gggccagctc gagcgacgcc gtcgtcatcg acgagatcat gtccgcgggg cccctcaggc
     1981 gtcgcagcgc cagcgtccga gctcggtcag ctgcgcgctg ccgggctgtt cgatcgacca
     2041 ggcgaagccg tcgtcgtcgt agctgttgag ccacagctcc ggccggccgt cggcgtcgag
     2101 gtcgaccacg ccgagcacgg tgtactgcac gccggccccc ggctcgccct tgatcttgcg
     2161 cgggcgggcg ccctcgaccg cgggcaccag gtacagcccc gcgaacgccg gcggcgcgtc
     2221 gtcctcaccg cctgtggccg gcagggtgac cgacgccagg ccctccggct tgcggtcgcc
     2281 gtcgaggtcg atctccgacc attgctgcag ttgcaagccc ttcgtgtcac cgagcgcggg
     2341 atagtcggtc gcgaccacgg cggcgatgct ggcgagcgtg cccgcgggcg ccgcctcggc
     2401 ggcctcggcc ggcgtgctcg gcgggaccgt ctcgacgaag ctcgggtcgg acggcgccgt
     2461 cgcgtgcccg cgcagggcct ccgcgggcgc ctcgacgacg agcgcctggc cgccgccgca
     2521 ggccgcggga ccggtgcccg tgaccttggc gagcgccccg gactcgaggc gaagctcggt
     2581 gccgaccggc gcgagcgcga ggcagggctc acccgtgctg aacttcggga catgtgcctc
     2641 gtgacaggcg atcgggatca ggccgctctc gctggcggcc aggaacaccc gaccgtgcgg
     2701 cttggcgggc ggcgcaggcg cgggc
//
LOCUS       DASWNJ010000193         2068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_179955, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000193 DASWNJ010000000
VERSION     DASWNJ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179955"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..819)
                     /locus_tag="VGB85_03595"
     CDS             complement(70..819)
                     /locus_tag="VGB85_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133130.1"
                     /translation="MSELTSTFESWLRRLVPGADRHWRGASDSEIAQVEALAGRPRPP
                     FYRWFLARMGHDMGALAYPDVDFSVRRVLACNGEGLFSPNPRYFMIGFVTDEMMPLHV
                     LYDFAHPARDDARVTRRHALVGPFHDRFETLHDMFAWGALLRFRVNPARQTCTGSLHD
                     DADDVAARLVPVMESLGFAAPFKAGAHCSLFERDDMVMITTSSSPAEPPRRHIFRLAG
                     SDSTAIRSVLGSILDATSLRLENPEWTPPLR"
     gene            complement(807..1031)
                     /locus_tag="VGB85_03600"
     CDS             complement(807..1031)
                     /locus_tag="VGB85_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133131.1"
                     /translation="MNDGHNIAAAMTEQWYHPKEAPTRGKVDFWDERDGPREFASDYF
                     PHGSTVPPCRACELIVPLMFCSGEGQKCPS"
     gene            complement(1028..1162)
                     /locus_tag="VGB85_03605"
     CDS             complement(1028..1162)
                     /locus_tag="VGB85_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133132.1"
                     /translation="MTHREGNVSYLDGQQSLDEKYADKIKILRKSLDARLGAEVVDPV
                     "
     gene            complement(1159..1458)
                     /locus_tag="VGB85_03610"
     CDS             complement(1159..1458)
                     /locus_tag="VGB85_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133133.1"
                     /translation="MVQAAGVVALEAGNCPVWGRNHGELKESKVTKADADGLAAKFKA
                     ETEATVAHGLTLKQKKKVYISPTMLGVVHCKCGNKYADQSAMRRRSSQVRPVRRK"
     gene            complement(1517..>2068)
                     /locus_tag="VGB85_03615"
     CDS             complement(1517..>2068)
                     /locus_tag="VGB85_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133134.1"
                     /translation="ANPTLPEPVFVLTIQFLERLITQDRDCRLMSKQLAARLAMVSSS
                     SGPATYREFRKVLPLRRRARKLLASVHARIDECLSQLERLADIVHDPWQLHCARHVFA
                     GRRAAVALAFAARDQDTWTAAVRTAVAALAALHAERPEHPEPLLWLTRFHVQLGERGE
                     ARKFAKLAAHLGERTALAELDQS"
BASE COUNT          356 a          654 c          706 g          352 t
ORIGIN      
        1 cccatcggat cagcccgccg actgccggcc gacggacctc cggcgagcgc gccgtggcca
       61 caagggcact caacgaagcg gaggcgtcca ctctggattt tcgagacgga gcgaggtcgc
      121 gtcgaggatc gatccgagga cactgcgaat ggccgtggag tcagaccctg cgagtctgaa
      181 gatatgccgc ctgggcggct ccgctgggct cgacgaggtc gtgatcatca ccatgtcgtc
      241 gcgctcgaag agcgagcagt gtgcaccagc cttgaatggg gccgcgaatc ccagagactc
      301 catgactgga acgaggcgcg ccgcgacgtc atcggcgtca tcgtggagcg aaccggtgca
      361 ggtctgccgc gcagggttca ccctgaagcg cagcaaggcg ccccaggcga acatgtcgtg
      421 cagggtctcg aaacggtcgt gaaacggccc gaccagggcg tgccggcgcg tgacccgggc
      481 atcgtcgcgt gcggggtgcg cgaagtcgta gagaacgtgc agcggcatca tctcatcggt
      541 cacgaagccg atcatgaaat accgagggtt tggagagaag agtccctcgc cattgcaagc
      601 gagcacgcgc cgcaccgaga agtccacgtc cgggtacgcc agtgcaccca tatcatgacc
      661 catgcgggcg agaaaccagc gatagaaggg gggtcgcggg cgaccggcaa gcgcctcgac
      721 ctgcgcgatc tcgctgtcgg aagcgccgcg ccagtgtctg tccgcaccag gcacgaggcg
      781 acgcagccag ctctcgaagg tggacgttag ctcggacatt tttgcccctc gccgctacaa
      841 aacatcagtg gaacgatcag ctcgcaggcc cggcacggtg gcaccgtcga cccgtggggg
      901 aaatagtccg aggcgaactc gcggggaccg tcgcgctcat cccagaagtc caccttgccg
      961 cgcgtcggag cttccttggg atggtaccac tgctcggtca ttgccgccgc gatgttgtgt
     1021 ccgtcgttca tacgggatct acgacctcgg cgccgagacg agcgtccagg ctcttccgca
     1081 atatcttgat cttgtcggcg tacttctcgt cgagcgactg ctggccgtcg aggtatgaga
     1141 cgttcccctc gcggtgtgtc atttccgccg aaccggccgc acttgcgaac tccgtcgtct
     1201 catcgccgac tgatcggcgt acttgttgcc gcacttgcag tgcacgacgc cgagcatggt
     1261 cgggctgatg tagaccttct tcttctgctt cagggtcagc ccgtgcgcga cggtggcctc
     1321 ggtttctgcc ttgaacttgg ccgctaaccc gtcggcgtcc gccttggtga ccttcgactc
     1381 cttcagttcc ccgtggttcc taccccaaac gggacagttg cccgcctcca gcgccacgac
     1441 gccagcagcc tggaccacac caagcatggt cgctgtcgcc gcgacggcgt cgcggcggat
     1501 ttaccagtgg agtgcctcac gactggtcga gctcggcaag tgccgtccgt tcgccgagat
     1561 gtgcggccag cttggcgaac ttccgtgcct ccccgcgctc gccgagctgc acgtggaagc
     1621 gggtcagcca gaggagaggc tcggggtgtt cggggcgctc ggcgtggagg gctgcgaggg
     1681 cagcgaccgc ggtgcgcacg gcggcggtcc aggtgtcttg gtcgcgcgcg gcgaatgcaa
     1741 gggcaactgc ggcgcggcgg ccggcgaaca cgtggcgtgc gcagtggagt tgccatgggt
     1801 cgtgcacgat atcggcgaga cgctcgagct ggctcaggca ctcgtcgatg cgtgcgtgca
     1861 cggacgcgag gagcttgcgg gcgcggcggc gcagcggcaa gactttgcga aactcgcggt
     1921 acgttgccgg acccgagctc gaagacacca tggccagcct cgcggcgagc tgtttggaca
     1981 tgagccggca atcgcggtcc tgggtgatga gccgctcgag gaactgaatc gtgagcacga
     2041 acactggctc gggcagcgtg ggattggc
//
LOCUS       DASWNJ010000194         2047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_427670, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000194 DASWNJ010000000
VERSION     DASWNJ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427670"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VGB85_03620"
     CDS             complement(<1..767)
                     /locus_tag="VGB85_03620"
                     /inference="COORDINATES: protein motif:HMM:NF016629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HEY0133135.1"
                     /translation="MTATPHDALFKAAFSQVVYAAEELRHVLPPELVAVIDFSTMQVE
                     PGSFVDEVLRARYTDLLYSLEVAGCDARVYVLFEHQSSGERWMALRLLRYMIAIWDGC
                     IADGATVLPVIIPVVLHHGDGGWTAATRFEDLFKMPPGAAEFTPHFRFAIDDLGASGE
                     AALHARAASAYTRLVLSALQQARSGTEVRRLLFGWVDLIHALLREPHGHNALGLIVQY
                     LLEVRGAGELGVIDTTLREITADNGEEVMQTIAQMLEA"
     gene            855..>2047
                     /locus_tag="VGB85_03625"
     CDS             855..>2047
                     /locus_tag="VGB85_03625"
                     /inference="COORDINATES: protein motif:HMM:NF023500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3556 domain-containing protein"
                     /protein_id="HEY0133136.1"
                     /translation="MFLKPVPPPYDPLEWEKLPFPEKARQACLSWAMQGYGTPLAIYI
                     VYALKIALYVWVWSLCCACTPGMGELADIATWWTRPEAFQKAILWSMLFELLGLGCGS
                     GPLTGRYLPPVGGALYFLRPGTTKLPLFPSLPLLGGFRRGLLDVALYAATLTLLTITL
                     VSAVPDPTHLLALAVLLPLLGVLDRTLFLCARGEHYWTTLMVFVLAGPGFIAGAAWVH
                     AALWFWAGVSKLNHHFPAVVGVMTSNNPFLRSQRLRRLMYRRYPDDLAPSRLAVVMAH
                     AGTALELGVPILLLAGDGGPVTLAGLVLMLLLHGFITSNVPMGVPLEWNVMMVYGGFY
                     LFHAHAAADLTAMTPATVALLLVMCVAVPVLGNFWPARVPFLLAMRYYAGNWAYSVWL
                     FRGDA"
BASE COUNT          276 a          768 c          651 g          352 t
ORIGIN      
        1 gcctccagca tctgcgcgat ggtctgcatg acttcttctc cgttgtcggc ggtgatctcg
       61 cgaagggtgg tgtcgatgac gccgagctcg ccggccccac ggacctcgag aagatactgc
      121 acgatcagcc cgagcgcatt atggccgtgc ggctcgcgca gcagcgcgtg gatgaggtcg
      181 acccagccga acagcagcct gcggacctcc gtgccgctgc gcgcctgttg cagcgccgac
      241 agcaccaggc gggtgtacgc cgaggccgcc cgcgcgtgca gggccgcctc gccgctggcg
      301 ccgaggtcgt cgatggcgaa gcgaaagtgc ggcgtgaact cggccgcccc gggcggcatc
      361 ttgaacaaat cctcgaagcg ggtcgcggcg gtccagccgc cgtcgccgtg atgcagcacg
      421 accgggatga tgaccggcaa caccgtcgcc ccatcggcga tgcatccgtc ccagatcgcg
      481 atcatgtagc gcagcagtcg cagcgccatc caccgctcgc cgctgctctg gtgctcgaac
      541 agcacgtaga cgcgagcgtc acaccctgcg acctcgagcg agtagagcag gtcggtgtag
      601 cgagcgcgca gcacctcgtc gacgaagctg ccgggctcga cctgcatcgt cgagaagtcg
      661 atcaccgcga cgagctcggg cggcagcacg tgccgcagct cctcggccgc gtacacgacc
      721 tgcgagaagg cggccttgaa cagggcgtcg tgcggcgtcg cggtcacggc gtcgcacgct
      781 agcgcgagat cgcgcggttg caagccacgg cctcacagcg cctccacagg cccgccccgg
      841 cgatacaatg caccgtgttt ctcaagccgg tcccaccccc ttacgacccg ctcgagtggg
      901 agaagctccc tttccccgag aaggcgcgcc aggcctgcct ctcgtgggcg atgcagggct
      961 acggcacccc gctggcgatc tacatcgtct acgccctcaa gatcgccctg tacgtctggg
     1021 tctggtcgct gtgctgcgcg tgcactccgg gcatgggcga gctcgccgac atcgccacct
     1081 ggtggacgcg ccccgaggcg ttccagaagg cgatcctgtg gagcatgctg ttcgagctgc
     1141 tcggcctcgg ctgcggtagt gggccgctga ccggccgcta tctgccgccc gtcggcggcg
     1201 ccctgtactt cctgcggccc ggcaccacca agctgccgct gttcccctcg ctgccgctgc
     1261 tcggcgggtt ccgccgcggc ttgctcgacg tcgccctcta cgccgcgacc ctgaccctcc
     1321 tcaccatcac gctggtctcg gctgtcccgg acccgacgca cctgctcgcg ctcgcggtcc
     1381 tccttccgct cctcggcgtc ctcgaccgca cgctgtttct atgcgcccgc ggcgagcact
     1441 actggaccac gctgatggtc ttcgtgctcg ccggcccggg cttcatcgct ggcgccgcgt
     1501 gggtccacgc cgccttgtgg ttctgggccg gcgtctccaa gctcaaccat cactttcccg
     1561 cggtcgtcgg cgtgatgacc agcaacaacc cgttcctgcg ctcgcagcgg ctgcggcgcc
     1621 tgatgtaccg ccgctacccc gacgacctgg ccccctcgcg cctcgccgtc gtgatggccc
     1681 acgccggcac cgcgctcgag ctcggcgtac cgatcctgtt actcgcgggc gacggcggtc
     1741 ctgtcacgct cgccggcctc gtgctgatgc tgctgctgca cggcttcatc accagcaacg
     1801 tgccgatggg cgtgccgctc gagtggaacg tcatgatggt gtacggcggc ttttacctgt
     1861 tccacgccca cgccgccgcc gacctcaccg cgatgacgcc cgcgaccgtc gcgctgctgc
     1921 tcgtcatgtg cgtcgccgta ccggtcctcg gcaacttctg gcccgcgcgc gtgccctttt
     1981 tgctggcgat gcgctactac gccggcaact gggcctacag cgtgtggctg ttccgcggcg
     2041 acgcgta
//
LOCUS       DASWNJ010000195         6281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_109755, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000195 DASWNJ010000000
VERSION     DASWNJ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6281
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109755"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="VGB85_03630"
     CDS             <1..952
                     /locus_tag="VGB85_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133137.1"
                     /translation="EVTPPASGHTAAPVPGTQHLGKPGDSILGAPTTEDSTSRGQAPG
                     PADSGVVASPGALDMSSTASGGPRNPFQKAGALAPGAASYVQRASDAALAAALAEHPL
                     IVLRGEFSIGKSSLLLRSDRYWARGSEAALCRLDLMDMRIDDVNLFYDEFFIAVGQCY
                     GETFQGWREISAAIERRPLLLTLDEFSGLTEAVAQRFVPALHRLGTRHVEQLRIVVTA
                     RDAFEAVLASFQLNNPNLGDWRTVEIGPFTAAELQSLLGLLPPRAKAAALGQIDLVRR
                     MSAMAPKPAQCLCFNLFKDELAGRSDAELAARVARKDSYR"
     gene            949..4320
                     /locus_tag="VGB85_03635"
     CDS             949..4320
                     /locus_tag="VGB85_03635"
                     /inference="COORDINATES: protein motif:HMM:NF025869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0133138.1"
                     /translation="MSLENPYRAAGAFSGKAYIRRDADAELIEQIEDNQYYPYFAAPR
                     QSGKSSLLLRTMAALDNKRYRCALVDLSPFVVSSYDDFWRQFLHEVARSANFDPAPIG
                     QEDPRDVLLLWLASCNQRLIVFMDEIDVLLSVDFREQIFSKFRTFFNLRARLDTASLQ
                     RLQFVFAGAAHSSRFIKDPRWSPFNVAIEIELEDLTPAQVTQLAAYLGTAGSFAGPDL
                     TARVFELSGGSVFLCQLIFEQLWNQAARGKQRLGAADVDSVVATIVAESPRNIHFYNI
                     FRLVTADARLTMLFRRLTEGQELGQEEAKELRLTGICRGANPFRNEIYARVFGPGGPL
                     DLKVGDRQIQLTKALDGAIARRDSLQGDPRAEPRAIAAAEAEIRRLRKEFREGTQLQA
                     GDVLGRGRYRLEERIGQGGFAGVWRATDLRDQSDVAIKVLHGQFAEDKSRRERFFRGA
                     WKMSELQHPAIVRVLEPYGDENGQFYFVMEYLPGGTLHAAVQDMQVTAEVALKMLAQV
                     GQALHFAHERGLVHRDVKPSNVMIDGERGKLTDFDLVRETDITGGTRTGAMGTFIYAA
                     PEVMSRPQDADVRADIYGLGMTVVFALYGADLAMDVIRDADRFIDRLPCNAVLKDVLK
                     KSVAWDRNDRFTSAAEFVAALERASEVEVDRVVPAASEKLAAREVVALGDDWDDDEFA
                     GPAPAASERAAAPVPVAAPVTVAARPSGDAPRSASAPARPPPPGSAAPPASAAGPSSA
                     PARPAAAPAGRAGEAKTSEQPARQAVSGYDDGDDRGSPAANAVAPVMLAGVGRRSEIA
                     RSRPESADLELADAPPAAVSLAEAARATGARTNEPVPGGRLRASAGPPPPAATPAMPE
                     SRSGSGLSLAIALTGLAAALLLVLFLPRLLWPEDATSGMELTARVEHAQQPTQVQPPL
                     HPELVLPGPGDPRSGSPTEAVGEASGALRVDPALTEPTSRGSEPTPVTDTTGEPIPTA
                     DPTNPAVSESGKSSDAVADPPDHNGARPPTIRVSEQIDEGCALVRGGDAKLGVEVLLK
                     AHDRKPGDMRLLLCLADGHSKLENFQGAANFYERVLEKSSRHRIALLGAAQAYEAMNH
                     RAAARVFYQQLLDQEPGNVSARAFFAEEAALARTP"
     gene            4876..5151
                     /locus_tag="VGB85_03640"
     CDS             4876..5151
                     /locus_tag="VGB85_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133139.1"
                     /translation="MAMSNMMRDDMDSAEIEADTDPNSEGYWAERGMKRPQLVWERLS
                     SSNNIETYRTRVPGGWLIMIAQEYRDCGGSFFYPDPTYRWDGTGYHG"
     gene            complement(5179..5811)
                     /locus_tag="VGB85_03645"
     CDS             complement(5179..5811)
                     /locus_tag="VGB85_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133140.1"
                     /translation="MTDFMTRHSPRGAILRSLSLISMTSGLYVGALAIPGQAEASVVG
                     RKGIEIEGPQDRRGFYVGAGLGFGAVFFRSPLFASTYKDADNVIPAGRLELSLGGGVT
                     KRFTLGANLYLGTYAGRNFGPKVLFGGDVEGYAFIVRGFFVRTGLGVAGLPGGDGRIH
                     LGMGGLLGLGYEFWLNQTAALSVNLNYDLRVVPGDGLRHTPYLGLRFAWY"
     gene            complement(5844..>6281)
                     /locus_tag="VGB85_03650"
     CDS             complement(5844..>6281)
                     /locus_tag="VGB85_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133141.1"
                     /translation="NTGTLVIDGQEHPLELATNLDAEIVREYEALKPKIIGAAITRLI
                     VRAAVAEGARAAGNQAKDGAGAMVGLIAALAAEGAMVAADKPDTRSWTLLPAFVFVAR
                     VPVEPGPHSVTVTAMGSGGRETHTFKIDVKAGGFAVLDVTTLR"
BASE COUNT          935 a         2133 c         2349 g          864 t
ORIGIN      
        1 cgaggtcacg ccgccggcgt cggggcacac cgcggcgccg gtgccgggga cgcagcacct
       61 cggcaagccg ggcgacagca tcctcggcgc gccgacgacc gaggacagca cgtcgcgcgg
      121 ccaggcgccc gggccggcgg acagcggggt cgtggcgtca cctggcgctt tggacatgtc
      181 gagcacggcc tcgggcgggc cgcgcaaccc gttccagaag gccggcgcgc tcgcgccggg
      241 ggctgcgtcg tacgtgcagc gggcgtcgga cgcggcgctg gcggccgcgc tcgcggagca
      301 cccgctgatc gttctccgcg gcgagttctc gatcggcaag agcagcctgt tgctgcgcag
      361 cgatcgctac tgggcgcgcg ggtcggaggc ggcgctgtgt cgcctcgacc tgatggacat
      421 gcgcatcgac gacgtcaacc tgttctacga cgagttcttc atcgcggtcg ggcagtgcta
      481 tggcgagaca ttccagggct ggcgcgagat ctcggcggcg atcgagcggc gaccgctgct
      541 gctgaccctc gatgaattct ccgggctcac cgaggccgtc gcgcagcgct tcgtgccggc
      601 gctgcatcgc ctcggtacgc gacacgtcga gcagctgcgg atcgtcgtca ccgcgcgcga
      661 cgccttcgag gcggtgctcg ccagcttcca gctcaacaac cccaacctcg gcgactggcg
      721 caccgtcgag atcgggccgt tcaccgctgc cgagctgcag agcttgctgg gattgctgcc
      781 gccgcgcgcg aaggcggcgg cgctgggcca gatcgacctc gtgcgcagga tgtcggcgat
      841 ggcgccgaag cccgcgcagt gcctgtgctt caacctgttc aaggatgagc tcgcggggcg
      901 cagcgacgcc gagctggcag ctcgggtcgc gcgcaaggac agctatcgat gagcctggag
      961 aacccgtatc gcgcggcggg agcgttctcc gggaaggcgt acatccggcg cgacgccgac
     1021 gccgagctga tcgagcagat cgaggataat cagtattatc cgtacttcgc ggccccacgg
     1081 cagagcggca agtcgagcct gctgctgcgc acgatggcgg cgctcgacaa caagcgctac
     1141 cgctgcgcgc tggtcgacct ctcgcccttc gtcgtctcgt cgtacgacga cttctggcgc
     1201 cagttcctgc acgaggtggc gcgcagcgcc aacttcgacc cggcgccgat cggccaggag
     1261 gacccgcgcg acgtgctcct gctgtggctg gcgagctgca accagcgatt gatcgtattc
     1321 atggacgaga tcgacgtgct gctgagcgtc gacttccgcg agcagatctt cagcaagttt
     1381 cgcacctttt tcaatctgcg cgcgcggctc gacacggcgt cgctgcagcg cctgcaattc
     1441 gtgttcgcgg gcgcggccca ctcgtcgcgc ttcatcaaag atccgcggtg gtcgccgttc
     1501 aacgtggcga tcgagatcga gctcgaggac ctgaccccgg cgcaggtgac ccagctcgcc
     1561 gcgtacctcg gcacggccgg cagcttcgcc gggccggacc tgacggcgcg ggtcttcgag
     1621 ctctcgggcg gctcggtgtt cctttgtcag ctgatcttcg agcagctgtg gaaccaggcc
     1681 gcgcgcggga aacagcggct cggcgcggcc gatgtcgatt cggtggtcgc gacgatcgtc
     1741 gccgagagcc cgcgcaacat ccatttttac aacatcttcc ggctggtcac cgcggacgcg
     1801 cgcctgacga tgctgttccg gcgattgacg gagggtcagg agctcggtca ggaggaggcc
     1861 aaggagctgc gcctgaccgg catctgccgc ggcgccaacc cgtttcgcaa cgagatctac
     1921 gcgcgggtgt tcgggcccgg cgggccgctc gacctcaagg tcggtgaccg ccagatccag
     1981 ctgacgaagg cgctcgacgg ggcgatcgcg cggcgtgaca gcctgcaagg cgacccgcgg
     2041 gccgagccgc gggcgatcgc cgcggcggag gcggagatcc ggcggctgcg caaggagttc
     2101 cgcgagggca cgcagctgca ggcgggggac gtgctcgggc gcggccgcta tcgtttggag
     2161 gagcgcatcg gtcaaggcgg cttcgcgggc gtgtggcggg cgaccgacct gcgcgaccag
     2221 agcgatgtcg cgatcaaggt gctgcacggg cagttcgcgg aggacaagag ccggcgcgag
     2281 cgcttctttc gcggcgcgtg gaagatgtcg gagctgcaac acccggcgat cgtgcgggtg
     2341 ctcgagccct atggcgacga gaacggccag ttctacttcg tgatggagta cctgcccggc
     2401 ggcacgctgc acgccgcggt gcaggacatg caagtgaccg cggaggtcgc gttgaagatg
     2461 ctggcgcagg tcggccaagc gctgcacttc gcccacgagc gtggcctcgt gcaccgcgac
     2521 gtcaagccga gcaacgtgat gatcgacggc gagcgcggca agctgaccga cttcgacctc
     2581 gtgcgcgaga cggatatcac cggtggcacc cgcaccgggg cgatggggac gttcatttat
     2641 gccgcgcccg aggtgatgtc gcggccgcag gacgccgatg tgcgcgccga catctacggc
     2701 ctcggcatga cggtggtgtt cgcgctgtac ggcgccgacc tggcgatgga cgtcatccgc
     2761 gacgccgacc gcttcatcga ccgcctgccg tgcaacgcgg tgctcaagga cgtgctcaag
     2821 aagtcggtcg cgtgggaccg caacgaccgg ttcacgtcgg ccgccgagtt cgtcgcggcg
     2881 ctcgagcggg cgtcggaggt cgaggtcgac cgggtggtgc cggcggcgag cgagaagctc
     2941 gcggcgcgcg aggtggtggc gctcggcgat gactgggacg atgacgaatt cgccggcccg
     3001 gctccggcgg cgagcgaacg tgcggcagcg ccggtcccgg tcgcggcgcc ggtgaccgtg
     3061 gccgcgaggc cctccggcga cgcgccacga tcggcttcgg cccccgcacg accgcccccg
     3121 cctggctcgg ccgcgccgcc tgcgagcgcc gcagggccga gctccgcgcc cgcgcgaccg
     3181 gccgcggcgc cggccgggcg cgccggcgag gcgaagacat cggagcagcc cgctcgccag
     3241 gcggtatccg ggtacgacga cggcgacgac cgggggagcc cggcagcgaa cgcggttgca
     3301 ccggtgatgc tcgcgggggt cgggcggcgc agtgagatcg caaggtccag gcccgagtcg
     3361 gccgacctcg agctcgccga tgcgccgccg gccgcggtct cgctcgctga ggccgcgcgc
     3421 gcgacggggg ctcgcacgaa tgagcctgtc cccgggggca ggttgcgtgc gagcgccggg
     3481 ccgccaccgc ccgccgcgac gccggcgatg cccgagtcgc gctcgggctc gggcctgtcg
     3541 ctggcgatcg cgctgaccgg cctggcggcg gccttgctgc tggtgttgtt ccttccgcgg
     3601 ctgctgtggc ccgaggacgc cacgtcgggc atggagctga cggcccgtgt cgagcacgcg
     3661 cagcagccga cccaggtgca gccgcccttg cacccggagc tggtgctgcc cgggcccggg
     3721 gatcccagga gcgggtcgcc gaccgaggca gtgggggagg ccagcggagc gctgcgcgtc
     3781 gacccggcgc tcacggagcc gacctcgcgg gggtccgagc cgacgccggt gaccgacacg
     3841 acgggcgaac cgatcccgac cgccgatccg acgaacccgg ccgtgagcga gtcgggcaag
     3901 agcagcgacg cggtcgccga tccgccggat cacaacggcg cgcgaccgcc gacgatccgc
     3961 gtcagcgagc agatcgacga gggctgcgcg ctggtccgcg gcggggacgc gaagctcggc
     4021 gtcgaggtgc tgctcaaggc gcacgaccgc aagcccggcg acatgcgcct gctgctgtgc
     4081 ctcgccgacg gtcactccaa gctcgagaac tttcagggcg cggcgaactt ctacgagcgc
     4141 gtgctcgaga agagcagccg gcacaggatc gcgttgctcg gcgcggcgca ggcctacgag
     4201 gcgatgaacc accgcgcggc cgcgcgggtg ttctatcagc agctgctcga ccaggagccg
     4261 gggaacgtgt cggcgcgggc gttcttcgcc gaggaggccg cgctcgccag gacgccctga
     4321 gccgcgtcac ttcgtgcctc gcggacgccg aagcggccgg ggtcgtcgac gcgaccgcgg
     4381 cgaagaccga gatcccgcgt cgatcgccgc cgcggtcgcc cggcaaaggc gcttcccatt
     4441 cgaggtcacg gtccaccccg acagcgaggg ccaggacgac cgcctccacg cgcgcggcgg
     4501 ctggcctccg gacgcgcgga tcaccgcggg ctcgacccgc aggcgggcat cgcaggcggc
     4561 cagcgggcga tcgaagcgcg cagatccacg gtctccctcg actggcccct ccggatcgcg
     4621 ctcgatcagc ttcagcgagc ccgcgggacc gggctgccgg gcgtcggtgt cggcgccacc
     4681 accgggatcc agggccgtct catcgcggga cctgcaacgg tcggggcgct cgtcgtgttc
     4741 cggccgcgat cggggtcgcc agacctcgcc agtgaacgcg tgtgctacgg gtgcccgaac
     4801 cggcccacag ggcctgcgcg cgaaagcgcc gacttcttgc tgcgcggcgg cgggccgtgt
     4861 aacgatgccg cggccatggc catgagtaac atgatgcgag acgacatgga ttccgccgag
     4921 atcgaggcgg acaccgatcc caacagcgag gggtactggg ccgagcgcgg catgaagcgg
     4981 ccgcagctgg tctgggagcg gctcagcagc agcaacaaca tcgagactta ccggacgcgc
     5041 gtcccgggcg gctggctgat catgatcgcc caggagtacc gcgactgcgg cggctcgttc
     5101 ttctatccgg atccgaccta tcgctgggac ggcaccggct accacggctg aggccgccgg
     5161 caggccgaag tcaccggctc aataccaggc gaagcgcagg ccgaggtacg gcgtgtggcg
     5221 caggccgtcg ccggggacca cgcgcaggtc gtagttgagg ttcacggaca gggcggcggt
     5281 ctggttgagc cagaactcgt agccgaggcc gagcaggccg cccatgccga ggtggatccg
     5341 gccgtcgccg ccgggcaggc cggcgacgcc gaggccggtg cgcacgaaga agccgcggac
     5401 gatgaacgcg tagccctcga cgtcgccgcc gaacagcacc ttggggccga agttacggcc
     5461 ggcgtaggtg ccgaggtaga ggttggcgcc gagggtgaag cgcttggtga cgccgccgcc
     5521 gagcgacagc tcgaggcggc cggcggggat gacgttgtcg gcgtccttgt aggtcgaggc
     5581 gaacagcggg ctgcgaaaga acacggcgcc gaagccgagg ccggcgccga cgtagaagcc
     5641 gcggcggtcc tggggtcctt cgatctcgat gcccttgcgg ccgacgacgc tggcctcggc
     5701 ctgacccggg atcgccaggg ctccgacgta gaggcccgag gtcatcgaga tcagggagag
     5761 cgagcgcagg atggcgccgc ggggggagtg acgggtcata aaatcggtca tggcgatgtc
     5821 cctttcgaag cgaatcaaag gcactaacgc agggtggtga cgtcgaggac ggcgaagccg
     5881 cccgccttga cgtcgatctt gaaggtatgg gtctcgcggc cgcccgagcc catcgcggtg
     5941 accgtgacgc tgtgcgggcc gggttcgacc ggcacgcgcg cgacgaacac gaacgcgggc
     6001 agcagggtcc agctgcgggt gtcgggcttg tccgccgcga ccatggcgcc ctcggccgcg
     6061 agcgcggcga tcaggccgac catcgcgccg gccccgtcct tggcctggtt accggcggcg
     6121 cgggccccct cggcgaccgc ggcgcggacg atcaggcgcg tgatcgcggc gccgatgatc
     6181 ttcggcttga gcgcctcgta ctcgcggacg atctccgcgt cgaggttggt cgccagctcg
     6241 agcgggtgct cctggccgtc gatgaccagg gtgccggtgt t
//
LOCUS       DASWNJ010000196         2232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_427700, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000196 DASWNJ010000000
VERSION     DASWNJ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427700"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2232
                     /locus_tag="VGB85_03655"
     CDS             <1..>2232
                     /locus_tag="VGB85_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133142.1"
                     /translation="DASHARVLTIDEIKKAGVRVDEIALGPLAISSITELLADTLRCP
                     TQQTRPLAELLLQKTGGNPFFLRQLLRALHDDGLLELDTTTGVWTWDVARIRDVGITD
                     NVVELMTAKLRRLPEATQDALKLAACIGNSFDLGMLSVVSERGAREVAARLWPAVREG
                     LIMRLSESYRVFQGDEGEPWAGDHGLTQGPEPGRPGESAAPERLRYRFLHDRVQQATY
                     ALIPADRRKIEHLKIGRLLAGGEHRDEDLFDIVAHLNVGRDLLGSEADRLELARLNYR
                     AGRRAKAATAMTVASEHFAVARELLPADRWTSHYELARDLHRELGECVYLLGEYDRSD
                     ELLSEAIAHGRDTYDQGTALECKMRLYLTQGPRDTRARAVATGLEALQVLGAAMPMTA
                     EARAARVVEVREAIARRLAQTDLEGLAGLPRMEDPQRLLVMTTTVTLFGAAYIIADMD
                     LLELCTYSIVDMSLDHGNSEASAFGYMLYGMLRSAQGEHRLAYELGRLGLSLHERYPN
                     PAMKPKIHNIFGHSINPFINHFETNLPHYRITYASCIESGDLLYGLWSVLYTLWVQIL
                     KGDNLQEIHDGSAAYMGFVERSGDANMLLSVQCLRQAVQCLRGLTDDLGSLDSLDSPD
                     SLDRGGFVEADLVRQFADSQNFSGQCWHALAREIVDVTFARYAEAVAHAAVAERTLGS
                     LYAFFNSTTHDCYAPLALAGAHDDAPVERQAEYLARIDEHLARLRLLADDCPDNFLHC
                     HQLV"
BASE COUNT          342 a          826 c          742 g          322 t
ORIGIN      
        1 gacgcgtcgc acgcgcgcgt gctgacgatc gacgagatca agaaggccgg cgtccgggtc
       61 gacgagatcg cgctcgggcc gctggcgatc tccagcatca ccgagctgct cgccgacacc
      121 ctgcggtgcc cgacgcagca gacccgcccg ctcgccgagc tcttgctgca aaagaccggc
      181 ggcaacccct tcttcctgcg ccagctgctg cgcgcgctgc acgacgacgg gctgctcgag
      241 ctcgacacca cgaccggcgt gtggacctgg gacgtcgccc gcatccgcga cgtcggcatc
      301 accgacaacg tcgtcgagct gatgaccgcc aagctgcggc ggctgcccga ggccacgcag
      361 gacgcgttga agctggccgc ctgcatcggc aacagcttcg acctcgggat gctgtcggtc
      421 gtgtccgagc gcggcgcgcg ggaggtcgcg gcgcgcctgt ggccggcggt ccgcgagggc
      481 ctgatcatgc ggctctcgga gtcgtatcgc gtgtttcagg gcgacgaggg cgagccctgg
      541 gcgggcgacc acgggctgac acaggggccg gagcccgggc gccccggcga gtcggcggcc
      601 ccggagcgcc tgcgttaccg gttcctgcac gaccgcgtgc aacaggcgac ctacgcgttg
      661 atccccgcgg accggcgcaa gatcgagcac ctcaagatcg ggcgcctgct cgccggcggt
      721 gagcaccgcg acgaggacct gttcgacatc gtcgcgcacc tcaacgtcgg acgcgacctg
      781 ctcggcagcg aggccgaccg cctcgagctc gcccggctca actaccgcgc cggccgccgc
      841 gcgaaggcgg cgacggcgat gacggtcgcc agcgagcact tcgccgtcgc acgcgagctg
      901 ctgccggccg accgctggac ctcgcattac gagctggcgc gcgacctcca ccgcgagctc
      961 ggcgaatgtg tatatctgct cggcgagtac gaccgctccg acgagctgct gagcgaggcg
     1021 atcgcgcacg ggcgcgacac ctacgatcag gggaccgcgc tcgagtgcaa gatgcggctg
     1081 tacctgaccc agggcccgcg cgacacgcgg gcgcgggcgg tcgccaccgg gctcgaggcg
     1141 ctgcaggtgc tcggcgccgc gatgccgatg accgccgagg cgcgggccgc ccgggtcgtc
     1201 gaggtccgcg aggcgatcgc gcggcggctc gcgcagaccg acctcgaggg cctggccggc
     1261 ctgccgcgca tggaggaccc gcagcggctg ctggtgatga cgacgacggt cacgctgttc
     1321 ggcgcggcct acatcatcgc cgacatggac ctgctggagc tatgcacgta ctcgatcgtc
     1381 gacatgtcgc tcgaccacgg caactccgag gcgtcggcct tcggatacat gctctacggc
     1441 atgctgcgca gcgcccaggg cgagcaccgc ctcgcctacg agctcggccg gctcgggctg
     1501 tcgctgcacg agcgctaccc gaaccccgcg atgaagccga agatccacaa catcttcggg
     1561 cacagcatca accccttcat caaccacttc gagaccaacc tcccgcacta tcgtatcacc
     1621 tacgcgagct gcatcgagag cggcgacctg ctgtatggat tgtggtcggt gctctatacc
     1681 ctgtgggtcc agatcctcaa gggcgacaac ttgcaggaga tccacgacgg ctccgcggcg
     1741 tacatgggct tcgtcgagcg ctccggcgac gcgaacatgc tgctatcggt gcagtgcctg
     1801 cgccaggccg tgcagtgcct gcgcgggctg accgacgacc tcggatcgct cgactcgctc
     1861 gactcgcccg actcgcttga tcgcggcggc ttcgtcgagg ccgacctcgt gcgccagttc
     1921 gcggactcgc agaacttctc cggccagtgc tggcacgcgc tcgcgcgcga gatcgtcgat
     1981 gtcacgtttg cgcggtacgc cgaggccgtc gcccacgcgg ccgtcgccga gcgcacgctc
     2041 ggctcgctct acgcgttctt caacagcacc acccacgact gctacgcccc gctcgccctc
     2101 gctggcgccc acgacgacgc tccggtcgag cgccaggccg aatacctcgc gcgcatcgac
     2161 gagcacctcg cccgcctgcg cctgctcgcc gacgactgcc cggacaactt cttgcactgc
     2221 catcagctcg tc
//
LOCUS       DASWNJ010000197         3668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_319917, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000197 DASWNJ010000000
VERSION     DASWNJ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319917"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1395
                     /locus_tag="VGB85_03660"
     CDS             <1..1395
                     /locus_tag="VGB85_03660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0133143.1"
                     /translation="IVLEGDEYDTAFFDKGSKFFHYAPFRAIVTSVEYDHADIFADLD
                     AVKAAFRRFIELIPAEGDLVVCADDAGAVEVAKAARCKVTTYRVLGDGDDPASADYTA
                     RASVKRGGGQTLFELFERGESQGEFATSLVGAYNVANIVAAVAMARKEGAPIEALRDG
                     VRRFRGVRKRQELLGIAAGIRVVTDFAHHPTAVKVTVTAVRRHFPNNKLHVCFEPRSA
                     SSRRAVFFEGYAEAFAAASRVYVGPLFSPGKIPPDQRLDPPQLARAIAQHGIEASAYA
                     SIDELGAAVLTQVAPGDTVLLLSSGSFGDLGSKLLQGLGDAFMFATAEDMPAVQALCS
                     SYGLAPTEYDDTTETLILREPEGDVVGCCSLKVAGTQALLFNLAVARSRRGEGLGWIL
                     ADGTIRRARNVGVRAIYLATSDAADFFANRVGFVAAPTEQVDPSLRESPHFAPIHQGG
                     VCMSFDPTWKPRPA"
     gene            1400..1882
                     /locus_tag="VGB85_03665"
     CDS             1400..1882
                     /locus_tag="VGB85_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133144.1"
                     /translation="MLATLLFAAYAIVALAAGVLVIAARRVRTAVEALFVSLLAGTCA
                     YVQVMAPVVAATQLVLLAGAAIAALRLAVTEEAPARFAWWRLVALVPVAGLALVLVST
                     WARQYVWTGRELPPGTRLGAAATIGQAWGELYAPALAVALLALLVAAIAGLATREHRL
                     "
     gene            1896..2156
                     /locus_tag="VGB85_03670"
     CDS             1896..2156
                     /locus_tag="VGB85_03670"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HEY0133145.1"
                     /translation="MTDLPITHLLYLASGLFVLGLVGVVLRRSPQRALLALVLLLSAT
                     GLVMATFARAWAQTGGQVFAILLVVLTAVYAVVGAALLRGRT"
     gene            2160..>3668
                     /locus_tag="VGB85_03675"
     CDS             2160..>3668
                     /locus_tag="VGB85_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133146.1"
                     /translation="MQAHVSLLAIVALPMLAGLLICGFEAAWRRWFGAERALALAGRI
                     ALGGTLLSGLLLAAGVYALRVRPDLGRTQEIPWAGAGHTTALDLDLELGGLALVAGAV
                     ALVVALGVQLAALGDVRDGARSLGRAALLLAGTLLLALSATLWGAALGWQIVVLVACS
                     WGQVREDMSEGAGAPRWRATDAGLWLAVLATTVGAGALAFAAIDRGALLGAQASLIAG
                     KLGGPFGAATPASVAAVGLAVAVLGRVAGLAGLLRGQSPVARAAVLGLASGAGMLLLL
                     RAHMLLVLAPTVMAALALVGGAVAVLAGVAALRTSSHEETLARVAQAQLGFILVAIGF
                     GAWAPACGLLLAYAVASAALGLGLGAGSGAGHAARVLAGLSLAAVLPTGATLWLGETL
                     GVAFMYMSAWSPGLNFVAAGLCAIAALCVAGSLAQVLRDRSHGPGGAEIGLASAFLAT
                     AAAIAALTDMPGSITALRVGLGPEFGPSWLLGGDLALGPRPSYSVVMARWAVV"
BASE COUNT          370 a         1229 c         1500 g          569 t
ORIGIN      
        1 atcgtgctcg agggcgacga gtacgacacg gccttcttcg acaagggctc gaagttcttc
       61 cactacgcgc catttcgggc gatcgtcacc agcgtcgagt acgaccacgc ggacatcttc
      121 gccgacctcg acgcggtcaa ggcggcgttt cgccggttca tcgagctgat ccccgccgag
      181 ggtgacctcg tggtctgtgc cgacgacgcc ggggcggtcg aggtcgcaaa ggcggcgagg
      241 tgcaaggtga cgacctaccg cgtgctcggc gacggcgacg acccggccag cgccgactac
      301 accgcgcgcg cgtcggtgaa gcgcggcggc ggtcagaccc tcttcgaatt gttcgagcgc
      361 ggcgagagtc agggcgagtt cgcgacctcg ctggtcggcg cgtacaacgt cgccaacatc
      421 gtcgcggcgg tggcgatggc ccgcaaggag ggcgccccga tcgaggcgct gcgcgacggt
      481 gtgcgccggt ttcgcggggt ccgcaagcgc caggagctgc tcggcatcgc cgcggggatc
      541 cgcgtcgtca ccgacttcgc gcaccacccg accgcggtca aggtgaccgt cacggcggtg
      601 cggcggcact tcccgaacaa caagctgcac gtgtgcttcg agccgcgctc ggcgtcgtcg
      661 cggcgcgcgg tgttcttcga gggctacgcg gaggcgttcg cggcggcctc gcgggtctac
      721 gtcggcccgc tgtttagccc cggcaagatc ccgccggacc agcggctcga cccgccgcag
      781 ctggcccggg ccattgctca gcacggcatc gaggcgtcgg cgtacgcgag catcgacgag
      841 ctcggcgcgg cggtgctgac ccaggtcgcg cccggcgaca cggtgctctt gctctcgagt
      901 ggatcgttcg gcgacctcgg cagcaagctg ctgcaggggc tcggcgacgc cttcatgttc
      961 gcgaccgcgg aggacatgcc cgcggtgcag gcgctgtgct cgagctacgg cctggcgccg
     1021 accgagtacg atgacacgac cgagacgctg atcctgcgcg agccggaggg cgacgtggtc
     1081 ggctgttgca gcctcaaggt cgcgggcacg caggcgctgc tgttcaacct cgcggtcgcg
     1141 cgcagccgtc gcggcgaggg cctcgggtgg atcctcgcgg acgggaccat tcgtcgcgcg
     1201 cgcaacgtcg gcgtgcgggc gatctacctg gcgaccagcg acgcggcgga cttcttcgcc
     1261 aaccgcgtcg gcttcgtcgc ggcgccgacc gagcaggtcg atccctcact gcgcgagagc
     1321 ccacacttcg caccgataca tcaaggcggt gtgtgcatgt ccttcgatcc gacctggaag
     1381 ccgcggccgg cctgagcgca tgctggcgac cctgctgttc gccgcgtacg cgatcgtcgc
     1441 cctcgcggcc ggcgtgctgg tgatcgcggc gcggcgcgtc cggaccgcgg tcgaggcgtt
     1501 gtttgtgtcg ctgctcgccg ggacctgtgc gtatgtgcag gtgatggcgc ccgtggtcgc
     1561 cgcgacgcag ctggtgctgc tcgcgggggc ggcgatcgcc gcgctgcggc tcgcggtgac
     1621 cgaggaggcg ccggccaggt tcgcgtggtg gcggctggtc gcgctggtgc cggtggcagg
     1681 gctcgcgctg gtgctggtgt cgacctgggc ccgccagtac gtgtggaccg ggcgcgagct
     1741 gccgccgggc acgcgcctgg gcgccgcggc gacgatcggg caggcgtggg gcgagctgta
     1801 cgcgccggcg ctggcggtcg cgctgctggc gttgctggtc gctgcgatcg cggggcttgc
     1861 gacgcgcgag catcggttat aaccgccgcg cgccgatgac cgatctgccg atcacgcacc
     1921 tcctctacct ggcgagcggg ctgttcgtgc tcggcctggt cggcgtggtg ctgcggcgct
     1981 cgccgcagcg cgcgctgctg gccctggtgt tgctgctgtc ggcgaccggc ctggtgatgg
     2041 cgaccttcgc ccgcgcgtgg gcgcagaccg gcggccaggt gttcgcgatc ttgctggtcg
     2101 tgctgaccgc ggtgtacgcg gtggtcggcg cggccctgct gcgggggcgc acgtaggcga
     2161 tgcaggcgca cgtcagcctg ctggcgatcg tcgccctgcc gatgctggcg ggcctgctga
     2221 tctgcgggtt cgaggcggcg tggcggcggt ggttcggcgc ggagcgggcg ctggcgctgg
     2281 ccggcaggat cgcgctcggc ggcacgctgc tgtcggggct gctgttggcc gcgggggtgt
     2341 atgcgctgcg cgtgcggccc gacctcgggc gcacgcagga gatcccgtgg gcgggcgcgg
     2401 ggcacacgac ggcgctcgac ctcgacctcg agctcggcgg gctcgcgctg gtcgccggcg
     2461 cggtcgcgct ggtggtcgcg ctcggggtcc agctggccgc gctcggggac gtgcgcgacg
     2521 gggcgcgcag cctcgggcgc gcggcgctgc tgctcgccgg cacgctgctg ctcgcgctgt
     2581 cggcgacgct gtggggcgcg gcgctcggct ggcagatcgt ggtgctcgtc gcgtgttcgt
     2641 ggggacaggt ccgcgaggac atgtccgaag gggcaggcgc gccgcgctgg cgggcgacgg
     2701 acgcggggct gtggctcgcg gtgctggcga cgacggtcgg cgcgggcgcg ctcgcgttcg
     2761 cggcgatcga tcgcggggcg ctgctcggcg cgcaggcgag cctgatcgcc ggcaagctcg
     2821 gcgggccgtt cggggcggcc acgccagcga gcgtcgcggc ggtcgggctg gcggtcgcgg
     2881 tgctcggcag ggtcgcgggg ctggcgggcc tgctgcgcgg gcagtcgccg gtggcgcggg
     2941 cggccgtgct cgggctggcc agcggcgccg gcatgttgct gctgttgcgc gcgcacatgc
     3001 tgctcgtgct ggcgccgacg gtgatggccg cgctggcgct cgtcggcggg gcggtcgcgg
     3061 tgctcgccgg ggtcgccgcg ctgcgcacga gcagccacga ggagacgctc gcgcgggtgg
     3121 cccaggcgca gctcggcttc atcctggtgg cgatcggctt cggcgcgtgg gcgccggcgt
     3181 gcgggctgct gctcgcgtac gcagtggcga gcgcggcgct cgggctcggg ctcggggcgg
     3241 gcagcggggc cgggcacgcc gcgcgcgtgc tcgcggggct ctcgctcgcc gcggtgctgc
     3301 cgaccggggc gacgctgtgg ctcggggaga ccctcggcgt cgcgttcatg tacatgagcg
     3361 cgtggagccc cgggctcaac ttcgtggccg cggggctgtg cgcgatcgct gcgctgtgcg
     3421 tcgcgggctc gctggcgcag gtgcttcggg acaggtccca tgggccaggt ggcgcggaga
     3481 tcgggctggc ctcggcgttc ctggcgacgg cggcggcgat cgccgcgctg acggacatgc
     3541 cggggtcgat cacggcgctg cgcgtcgggc tcgggccgga attcgggccg agctggctgc
     3601 tcggcggaga cctcgcgctc gggccgcggc cgtcatacag cgtggtgatg gcgcgctggg
     3661 ccgtggtc
//
LOCUS       DASWNJ010000198         3651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_427747, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000198 DASWNJ010000000
VERSION     DASWNJ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427747"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VGB85_03680"
     CDS             complement(<1..342)
                     /locus_tag="VGB85_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133147.1"
                     /translation="MSRLVLPLALASSFALAPTRAGAEVADADFTPTHTQQLVGLDDF
                     TFDTDWFPVDQPVQLRLIVHGGNSVAIDMPGDGLYDWDAGTISFAGDVDAGTFGVDVG
                     FTLDAKVRFDVF"
     gene            516..1871
                     /locus_tag="VGB85_03685"
     CDS             516..1871
                     /locus_tag="VGB85_03685"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0133148.1"
                     /translation="MPVASPMPVAAPVSAVPTSPVPPEVAAMLAEVSPQQLQATLTQL
                     VSFGTRHTLSEVASETRGIGAARRWIKAEMERGAQASGRVEDRAMRVELDVHVLPPDG
                     KRIDREVEVVNVVAVLPGNMPEARERRYYVVGHYDSRVGDVMDHESDAPGANDDGSGT
                     ALVIELARVMARHEFDATLVFMATAGEEQGLLGARGHAEAARRAGLKISGVLSNDIVG
                     DPGDPRGGTDHRAQIRVFSEGLPVPLADKELAQARQVSALGEAPSRQLARYVGEVAAR
                     QGLAVRPVLVFRPDRFLRGGDHTAFSDLGFAAVRFTEVAEKYDRQHQEVRVVEGRHYG
                     DTLEHVDVEYLAGVARLNAAALAHLARAPSVPGDVQILADALSIDTTLRWTASPEPDV
                     AGYEVLRRATTAPDWEYAQDFGKATVATLPYSKDDWLFAVRAYDTAGYRSPAVYPTLA
                     R"
     gene            complement(1940..2221)
                     /locus_tag="VGB85_03690"
     CDS             complement(1940..2221)
                     /locus_tag="VGB85_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133149.1"
                     /translation="MSNWTKIFTLSLFTAALAVPTAAQADEPAPKEPVEGKGNDNTND
                     SKTDKAPGGGTAEEPVPPAAGGKPGKGKGKGGKGGKGGKGKKDKEPPTE"
     gene            2608..>3651
                     /locus_tag="VGB85_03695"
     CDS             2608..>3651
                     /locus_tag="VGB85_03695"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0133150.1"
                     /translation="MSRSGAQQSPIQLITVGDFDPEADRGIEFDFIGREIAGRYTVRA
                     QIGGGGMADVYRATDEQLGIDVALKLLKPRMASDELRARMVQEAQAAARVRHPNLVRV
                     FGTGALDSTAYIAMELLEGPNLEQYLQEYRDGRMPCQEALELLLPALDALHEIHQRGY
                     VHRDIKTGNIIVVRSPGRPAAAIVIDLGLIKPDRALRNATSPPTTEVGRMLCTPGYTS
                     PEQAAGKPVDRRSDIYSMAVTLYRVLAGRLPFKDARGQPLALLAKHIYNIPTMLAEAA
                     GTARVPGAVAAVVERALSKDPARRPQTMLEFADELRAAAQPDVSRTAGLRRVRDVLLG
                     FGVATALALFVRPS"
BASE COUNT          534 a         1182 c         1359 g          576 t
ORIGIN      
        1 gaacacgtcg aagcgcacct tggcgtcgag ggtgaagccg acgtcgacgc cgaaggtgcc
       61 ggcgtcgaca tcgccggcga acgagatcgt gccggcgtcc cagtcgtaga ggccgtcgcc
      121 cggcatgtcg atcgcgaccg agttgccgcc gtgcacgatc aggcgcagct gcaccggctg
      181 gtcgaccggg aaccagtcgg tgtcgaaggt gaagtcgtcg aggccgacca gctgctgggt
      241 gtgggtcggc gtgaagtcgg cgtcggcgac ctcggcaccg gcccgcgtgg gggccagggc
      301 gaacgacgac gcgagcgcga gaggaagtac aagccgcgac atccgccgat gatacatggc
      361 ccgtgcgtcg gcgtgcgtcg gcgtggtggc tgccggtgcg tgtaggatca caggctttcg
      421 tgcgagtgtg gcatgatgcg gggatgcagc gccgggtcct cgcagtgcct ctcctcgcgt
      481 cgctcgcctg tggaccgcgt tcgcaggcgg tgccgatgcc cgtggcgagc ccgatgccgg
      541 tggccgcgcc ggtgagcgcc gtgccgacgt cgccggtccc gcccgaggtc gcggcgatgc
      601 tggcggaggt cagtccgcaa caattgcagg cgacattgac acagctggtg agcttcggga
      661 cccggcacac gctgtcggag gtcgccagcg agacgcgtgg catcggggcg gcgcggcggt
      721 ggatcaaggc ggagatggag cgcggggcgc aggcgagcgg gcgcgtcgag gaccgggcga
      781 tgcgcgtcga gctcgacgtg cacgtgctgc cgccggacgg caagcgcatc gatcgcgagg
      841 tcgaggtggt caacgtggtc gctgtattgc cggggaatat gcccgaggcc cgcgagcggc
      901 gatactatgt ggtcgggcat tacgactcgc gcgtcggcga cgtgatggac cacgagtcgg
      961 acgcgcccgg ggccaacgac gacggctcgg ggacggcgct ggtgatcgag ctggcgcggg
     1021 tgatggcgcg ccacgagttc gacgcgacgc tggtgttcat ggcgaccgcc ggcgaggagc
     1081 aggggctgct cggggcccgg gggcacgcgg aggcggcgcg gcgggccggt ttgaagatct
     1141 ccggggtgtt gagtaacgac atcgtcggcg accccggcga tccgcgcggc ggcaccgacc
     1201 atcgcgcgca gatccgggtg ttttcagagg ggctgccggt gccgctcgct gacaaagagc
     1261 tggcgcaggc gcggcaggtg tcggcgctcg gcgaggcgcc gtcgcggcaa ctcgcaaggt
     1321 atgtcgggga agtggccgcg cgacaggggc tcgcggtccg gccggtgctg gtgtttcggc
     1381 cggatagatt tttgagaggg ggcgaccata cggcgtttag tgacctcgga tttgccgcgg
     1441 tgcgattcac cgaggtcgcg gagaagtacg atcgccagca ccaggaggtc cgcgtggtcg
     1501 aggggcgaca ctatggtgat acgctggagc acgtcgatgt cgaatatctc gccggggtcg
     1561 cgcggctcaa cgccgccgcg ctcgcgcacc tggcccgcgc gccgtcggtg cctggcgatg
     1621 tgcagatact ggccgacgcg ctgagcatcg acacgacgct gcgctggacc gccagcccgg
     1681 agcccgacgt cgccggttat gaggtgctgc ggcgggcgac gaccgcgccg gactgggaat
     1741 acgcgcagga ttttggaaaa gcgacggtgg cgacgctgcc gtacagcaag gacgattggc
     1801 tgttcgcggt gcgggcgtat gatacggcgg gctatcgcag cccggcggta tatccgacgc
     1861 tggcgcgctg agggtgaccg gtgcgagccg gaggggctcg atcgggctcg aagccctgtg
     1921 atgcgaccat cctgagctac tactcggtcg ggggctcctt atccttcttg cccttgccgc
     1981 ccttgccgcc cttgccgccc ttgcccttgc ccttgcccgg cttgcccccg gccgctggcg
     2041 gaacgggttc ctctgcagtc ccgccgcccg gcgccttgtc agtcttacta tcgttcgtat
     2101 tgtcgttgcc cttgccctcg acgggctcct tgggcgcggg ctcgtcggct tgcgctgcag
     2161 tggggacggc gagggccgcg gtgaaaaggg agagggtgaa gatcttggtc cagttcgaca
     2221 tgagtgatgc tccaggacga aggtgcggtt cgacggctgt cgaccgcgtg caattgttga
     2281 cagataacgt ctataccggg catcgatcga ccgcccagcc tcgccgtcga tcgttgaatc
     2341 gcatggggct cgtggcgctg tcaatgttcg tattggccat ctttcccgcc gggcgcccgc
     2401 ccagcgcccg cccagcgcct attcatgaga gcatcgttgc gcaccggggt cactatcggc
     2461 gcccgcgata tgtgtatggt tgcaatctgg gtggccgtcc gcccggcgcg ctacccggcg
     2521 cgaagcgccg aggatcgcgc tggacacggg cgccatcggg ccggtatgtt tccgcccggc
     2581 gcgaagtcgc gcgtgggagg tccatccatg tcccgctccg gtgcgcagca gagccccatc
     2641 cagttgatca ccgtcggcga cttcgacccg gaggcggaca ggggcatcga gttcgacttc
     2701 atcggccgcg agatcgccgg ccgctatacg gtgcgggcac agatcggcgg tgggggcatg
     2761 gccgatgtct accgggccac cgacgagcag ctggggatcg acgtggcgct caagctgctc
     2821 aagccacgca tggcgtcgga cgagctgcgc gcgcgcatgg tccaggaggc gcaggcggcc
     2881 gcgcgcgtgc gccacccgaa cctcgtgcgg gtgttcggca ccggagcgct cgacagcacc
     2941 gcgtacatcg ccatggagct gctcgagggc ccgaacctcg agcagtatct acaggaatat
     3001 cgcgacgggc gcatgccgtg ccaggaggcg ctggagctgc tcttgccggc cctcgacgcc
     3061 ctgcacgaga tccaccagcg gggctacgtg caccgcgaca tcaagaccgg gaacatcatc
     3121 gtcgtgcgct cgcctggacg gccggcggcg gcgatcgtga tcgatctcgg gctgatcaag
     3181 ccggaccggg ccctgcgcaa cgccacgagc ccgccgacca cggaggtggg ccgcatgctc
     3241 tgtacgcccg gctacacctc gcccgagcag gccgccggca agccggtcga ccggcgctcg
     3301 gacatctact cgatggcggt cacgctctac cgcgtcctcg ccggccggct gcccttcaaa
     3361 gacgcccgcg gccagccgct ggcgctgctc gccaagcata tctacaacat cccgacgatg
     3421 ctcgccgagg ccgccgggac cgcgagggtc cccggcgcgg tcgcggcggt cgtcgagcgc
     3481 gcgctgagca aggaccctgc caggcgaccg cagacgatgc tggaattcgc ggacgagctg
     3541 cgcgctgcgg cccagccaga cgtttcgcgg accgccgggc tgcgccgggt ccgtgacgtc
     3601 ctcctcggat ttggggtcgc cacggcgctg gccttgttcg tccgcccgtc g
//
LOCUS       DASWNJ010000199         2971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_39186, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000199 DASWNJ010000000
VERSION     DASWNJ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39186"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1829
                     /locus_tag="VGB85_03700"
     CDS             <1..1829
                     /locus_tag="VGB85_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEY0133151.1"
                     /translation="EACLKEGTVHSSVLADKLGQPTKFELYYDFEESIAAIPSHRFLA
                     VRRGEQEGVLRVKIGLPPEAVTPRLEGKVGLAPASPFAGELAAAVRDGYVRLLAPSIE
                     TDLRIELKMKADRDAVEVFAQNLRQLLLAAPLGGKAVIGVDPGLRSGCKCVALDATGG
                     YHDTVTIFPGQGAGSDARARHDLLAFVRKHGPSAIAVGNGTGGREAEALVREVLAEAG
                     LSDILVVAVNEAGASVYSASDVAREEFPELDLTLRGAISIARRLQDPLAELVKIDPKA
                     IGVGQYQHDVYQPLLARKLDQVVESCVNHVGVDLNTASAPLLARVAGLRAGVARSIVE
                     HRSKHGAFRSRAALLGVAGLGPRSFEQAAGFLRIRGGEHPLDASAVHPERYPLVERIA
                     GDLGVDLRTLVGDATLAARVVVEKYVGDGVGEPTLRDILAELQKPGRDPRDRFDPPKF
                     RADIKTLADLKPGLVLEGVVTNVTAFGAFVDLGVHQDGLVHISQLSDRFVKDPHAVVK
                     AGEKLRVRVLEVDPERKRISLTARLGEPAQGSTGPGASTGKSAPTPGDRKQGDPGQRG
                     QHGKGGRDERGGGDRSRPAERSGPSQSDSGFRNNPFAALRKP"
     gene            1897..2508
                     /locus_tag="VGB85_03705"
     CDS             1897..2508
                     /locus_tag="VGB85_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133152.1"
                     /translation="MTDQDPAIRARYIRAHYAKLALKRLRAYPEGKGAEIRERVGPAL
                     LAAIRELAAPAWLAITDFVAVCEAIHGVLGEDATRAFWTDLMRDSYDHGMLKPLAVQA
                     QQTPGPAAAARLLATAPQAWQLSTQGCGSIELVEVAGKPRVRGVDLIPEILHSRGFVC
                     VFYGACKAMLELFKSRGEVRVSEHLPPGAERPQIAFDIVFEPG"
     gene            2597..>2971
                     /locus_tag="VGB85_03710"
     CDS             2597..>2971
                     /locus_tag="VGB85_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133153.1"
                     /translation="MFKPVFIATFLGLLLAADAGCKKKEQSEQPDDVAADEGAPKKSK
                     GVSEDNEDSLISSPTASAESGEAAPATTDMDSAVAKEEGGKKRPEVKEVCKKKTTGKG
                     KAKKTETVCEQVDSNPKLTASIG"
BASE COUNT          460 a         1043 c         1077 g          391 t
ORIGIN      
        1 gcgaggcgtg tctcaaggag ggcacggtgc acagctccgt gctggccgac aagctcggcc
       61 agccgaccaa gttcgagctt tattatgact tcgaggagtc gatcgccgcg atcccgtcgc
      121 atcggttttt ggcggtgcgg cgtggcgagc aggagggggt gctgcgcgtc aagatcgggc
      181 tgccgccgga ggcggtcacg ccaaggttag agggcaaggt cggcctcgct ccggcgtcgc
      241 ccttcgccgg tgagctggcc gcggcggtgc gcgacggcta cgtgcgcctg ctggcgccga
      301 gcatcgagac cgacctgcgg atcgagctca agatgaaggc cgaccgcgac gccgtcgagg
      361 tgttcgccca gaacctgcgc cagctgttgc tggcggcgcc gctcggcggc aaggcggtga
      421 tcggcgtcga cccgggcctg cgcagcggct gcaagtgcgt cgcgctcgac gcgaccggtg
      481 gctatcacga caccgtcacg atcttccccg ggcagggcgc cgggtccgac gcccgcgcgc
      541 gccacgactt gctcgccttc gtgcgcaagc acgggccctc ggcgatcgcc gtcggcaacg
      601 gcaccggcgg gcgcgaggcc gaggcgctcg tgcgcgaggt cctcgccgag gctggccttt
      661 cggacatcct cgtcgtcgcc gtcaacgagg ctggcgcgag cgtgtactcg gcctcggacg
      721 tcgcgcgcga ggagttcccg gagctcgacc tgacgctgcg cggcgcgatc tcgatcgcgc
      781 gccggctgca ggacccgctc gccgagctgg tgaagatcga ccccaaggcg atcggcgtcg
      841 gccagtacca gcacgacgtg tatcagccgc tgctggcccg caagctcgac caggtggtcg
      901 agagctgcgt caaccacgtc ggcgtcgacc tcaacaccgc gagcgcgccg ctgctggccc
      961 gcgtcgccgg cctgcgcgcg ggcgtggccc gcagcatcgt cgagcaccgg agcaagcacg
     1021 gcgcgtttcg ttcgcgcgcc gcgttgctcg gcgtcgcggg cctcgggccc cgcagcttcg
     1081 agcaggcggc cggcttcctg cggatccgcg gcggcgagca cccgctcgac gccagcgcgg
     1141 tgcacccgga gcgctacccg ctcgtcgagc ggatcgccgg cgacctcggg gtcgacctgc
     1201 gcacgctcgt cggcgacgcg accctcgcgg cccgcgtcgt cgtcgagaag tacgtcggcg
     1261 acggcgtcgg cgagccgact ctgcgcgaca tcctggccga gctgcaaaag ccgggtcgtg
     1321 atccgcgtga ccgcttcgac ccgccgaagt tccgcgccga catcaagacg ctcgcggacc
     1381 tcaagcccgg gctcgtgctc gagggcgtgg tcaccaacgt aacggccttc ggcgccttcg
     1441 tcgatctcgg ggtgcatcag gacggcctcg tgcacatctc gcagctgagc gaccgcttcg
     1501 tgaaggaccc gcacgcggtc gtgaaggccg gcgaaaaatt gcgggtgcgt gtgctcgagg
     1561 tcgaccccga gcgcaagcgg atcagcctga cggcccgcct cggcgagccg gcacaggggt
     1621 ccacggggcc aggggcgagc acgggcaaaa gcgcgccgac gcccggcgac cgcaagcagg
     1681 gcgaccccgg acagcgcggc cagcacggca agggcgggcg cgacgagcgg ggtgggggcg
     1741 atcgctcgcg cccggccgag cgcagcggcc catcgcagag cgactcgggg tttcgcaaca
     1801 accccttcgc cgcgctccga aaaccctgag ggtctcgcgc ggaccgatgt tcgcgggcat
     1861 gccggcggca tccttgcagt atcatcggcc agcgccatga ccgaccagga ccccgcgatc
     1921 cgagcgcgct acatccgagc ccattacgcc aagctcgccc tcaagcgcct gcgcgcctac
     1981 ccggagggca agggcgccga gatccgcgag cgcgtcggac cggcgctgct cgccgcgatc
     2041 cgcgagctgg cggcgccggc gtggctggcg atcaccgact tcgtcgccgt ctgcgaggcg
     2101 atccacggcg tgctcggcga ggacgccacg cgcgccttct ggaccgacct gatgcgcgac
     2161 agctacgacc acggcatgct caagccgctg gcggtgcagg cccagcagac gccgggcccg
     2221 gccgccgcgg cgcgcctgct ggcgacggcg ccgcaggcct ggcagctgtc gacgcagggc
     2281 tgcgggagca tcgagctggt cgaggtcgcc ggcaagccgc gggtccgcgg cgtcgacctc
     2341 atcccggaga tcctgcatag ccgcgggttt gtctgcgtgt tctacggcgc ctgcaaggcg
     2401 atgctcgagc tgttcaagag ccgcggcgag gtccgcgtct cggagcacct gccgccgggc
     2461 gccgagcgcc cgcagatcgc cttcgacatc gtgttcgagc cgggctagct cacgcaaagt
     2521 gcggcatcgc ccgcgcgcga ggccgtgacc cgcaaggcgt cacgtgttaa cgtggcggcc
     2581 ttcagggagc catcgaatgt tcaagccagt gttcatcgcc actttcctcg ggcttttgct
     2641 cgcggctgat gcgggttgca agaagaagga gcagtccgag cagcctgacg acgtggcggc
     2701 ggacgagggc gcgccgaaga agtccaaggg cgtcagcgag gacaacgagg acagcctgat
     2761 ctcgtcgccg accgcgtcgg ccgagtccgg cgaggccgcg cccgcgacga ccgacatgga
     2821 cagcgccgtc gccaaggaag agggcggcaa gaagcgcccc gaggtcaagg aggtctgtaa
     2881 gaagaagacc accggcaagg gcaaggccaa gaagaccgag acggtctgcg agcaggtcga
     2941 cagcaacccg aagctgaccg ccagcatcgg c
//
LOCUS       DASWNJ010000200         6162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_390861, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000200 DASWNJ010000000
VERSION     DASWNJ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390861"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VGB85_03715"
     CDS             complement(<1..626)
                     /locus_tag="VGB85_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133154.1"
                     /translation="MTISRTLHAHALSLTSLLALACGGSPVEGTASDSQSSSGAADAT
                     ATTGAVPTSTTATEANSATEAATQGTMSASGTEATTGTAGTLTEGVTSTDSATDTGTT
                     GTGTTGDTSTTDTTNTTNTTGTSDTTTSGTTGEPGSTGDTTAGDTTTSDTTDSTTGEN
                     VECGTQLKATIRDFKLGHPDFETYCCGLVAGLVKPDLGADKKPVFNQAG"
     gene            731..1207
                     /gene="rnhA"
                     /locus_tag="VGB85_03720"
     CDS             731..1207
                     /gene="rnhA"
                     /locus_tag="VGB85_03720"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEY0133155.1"
                     /translation="MATTTAPVPLDYVKLFTDGACKGNPGPGGWAFILQHPASGKAHE
                     GSGGEAHTTNNRMELMAVIRGLEMLRLPLTVDLFSDSQYVLKGMREWMAGWKRSGWRT
                     ADRQPVKNQDLWMALDELQQKHTLRFHWVKGHSGHPENERCDRMAVAARNLAGRGP"
     gene            complement(1211..2119)
                     /locus_tag="VGB85_03725"
     CDS             complement(1211..2119)
                     /locus_tag="VGB85_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133156.1"
                     /translation="MTSGTPLPGRSRRAALLVTLRTWLLPVALGAAAFGLHYAMHVPP
                     GPLPPPSPAELEEKKKAAEKKKKDDEKKQKEADKKAGKVTKPERTGPRELAYEPFSRP
                     RERYLLEQLWAYYEPIGFSKEPTFEAWQTAHKPLISQIVSATRQAVFAADGPAISVAA
                     SECHTIRCRFTITAPTAEALEQMSAALADLRLGEGSLWHSFKPGKVVEEPSKREGVDP
                     KQKLEFTVGFMRDLPALAHIEVPGKGRLRPPPTPSRPAAVPTTAPTGAPTTGPQPGSA
                     SAPPSAPAGPKRPDPKKPVELEPTPK"
     gene            complement(2116..2688)
                     /locus_tag="VGB85_03730"
     CDS             complement(2116..2688)
                     /locus_tag="VGB85_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133157.1"
                     /translation="MTCPEDQHGLVPANQPQSAGIEIATPQGTVTIALHPVLRTTLLI
                     AWAALSGACREQGADPVRTAQAFTAAVQRGDMKAVLPLLEKAAADHLAAVADKASDQV
                     GGRRRIEPAEMLQIVDVSPTFELAQTQLVDNDGATAHVRLVGAQQESYTLTLVQEDGA
                     WHVRIPTPASVSLIPSVPSVPSVPSVPSVP"
     gene            complement(2688..4097)
                     /locus_tag="VGB85_03735"
     CDS             complement(2688..4097)
                     /locus_tag="VGB85_03735"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY0133158.1"
                     /translation="MPFKPHEPIEASLTTTFGQTQHSRSLRRVMLRVMDGPDAGAQIQ
                     AARARLTVGRSAVNDLVLTDSSISGLHAELLVSKGGVLLRDLQSTNGSFVHGVRVQEA
                     WVEPGMTMVLGKTAIAFLAEDEVHVPLSGRDHFGAMLGTSPAMREIYAVLERVAGTEM
                     SVLVGGETGAGKELVARALHDESPRHQGPFVVLDCGALPRELAEAAILGHKKGSFTGA
                     MADRAGCFEEASGGTLFLDEIGELPLDLQPKLLRVLDRREVQRIGESTVRKVDVRIVA
                     ATHRDLRNMVSHGGFREDLYFRLSVMAVELPPLRDRGEDIILLAEKFIADTARRMPGK
                     PAPALSAAARQALLRHAWPGNVRELKNTIERATYLATHGIIEPTDLLLHRPAAARPAE
                     PAPAASTPAGDLSALFELPFKEAKQALLDDFETRYFTHLLEAHDGNLSRASAAAGITR
                     YYLRELLKRLGLHRVKEDE"
     gene            complement(4105..4785)
                     /locus_tag="VGB85_03740"
     CDS             complement(4105..4785)
                     /locus_tag="VGB85_03740"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY0133159.1"
                     /translation="MARSSVLASLPRFHRGGFWLLIIAAAASALLADHPLAGTLTLNL
                     GFTDRALWQPATAIFLYPDGQLGGLIGTLFIQWFIGGHVEERWGTARYLTLVLLSAFV
                     GYLAVALLGLAVPQVLAAPMGGTLPADIAAVVAFGVLHARQPVQLFGALPLSGRGLAA
                     LVTALVLLGPLLRGNWPVAVPTAVAALCGLLLAWRWRTPPGSGKVAARAGRRARHLKV
                     VDKPPLLN"
     gene            4902..5216
                     /locus_tag="VGB85_03745"
     CDS             4902..5216
                     /locus_tag="VGB85_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133160.1"
                     /translation="MSSKNRRQHRMFETLHTEYHLRLDECVGVRDKHSGEWYRDHPAL
                     RLRAIQVPSVGADEEFIGRRLQFWGEFDDVTTSPVVGVDRPHRESLYGYVSFARSGEI
                     AA"
     gene            5287..5775
                     /locus_tag="VGB85_03750"
     CDS             5287..5775
                     /locus_tag="VGB85_03750"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEY0133161.1"
                     /translation="MSAGTVCPQCPGSALSPMELDRGRLHRCFDCRGVWLERDAAVAM
                     FGGRPCHFEPPASDGAQCSVCRGAMAGLYFIGEAPVRVCGSHGVWLGPTDRVLLPEPL
                     QRKLKRRDFVDELLGWFESDDVSSGPGAGGSSEYFEVSASDGGGDGGASCGGCGGCGG
                     GD"
     gene            complement(5958..>6162)
                     /locus_tag="VGB85_03755"
     CDS             complement(5958..>6162)
                     /locus_tag="VGB85_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133162.1"
                     /translation="PTADTAAAGTPTADPSAAPATTASAPSTTPSSTTPSTTKTADPS
                     KGATKTGDATKGATTKGAAKKAE"
BASE COUNT          814 a         1916 c         2485 g          947 t
ORIGIN      
        1 ccggcctgat tgaacacggg cttcttgtcg gcgccgaggt cgggcttgac gaggccggcg
       61 accaggccgc aacagtaagt ctcgaagtcg gggtggccga gcttgaagtc gcggatcgtc
      121 gccttgagct gggtgccgca ctcgacgttc tcgccagtgg tgctgtcggt ggtatcgctc
      181 gtggtggtat cgccagcggt agtatcaccg gtgctgccgg gctcgccagt ggtgccgctc
      241 gtggtggtgt cggacgtgcc agtggtgttc gtggtgttcg tggtgtcggt ggtgctcgtg
      301 tcgccggtgg tgccagtgcc ggtggtgccg gtatcggtgg cgctgtcggt gctcgtgacg
      361 ccctccgtga gcgtgccggc ggtgccggtg gtcgcctcgg taccgctcgc gctcatggtg
      421 ccctgggtgg ccgcctcggt ggcgctgttg gcctcggtgg cggtggtgct ggtcggtacg
      481 gcgccggtgg tcgcggtcgc gtcggcggcg ccgctggagc tctggctgtc gctggcggtg
      541 ccctcgacgg gggagccgcc gcaggcgagc gcgagcaggg aggtcagcga gagggcgtgc
      601 gcgtggagcg ttctactaat tgtcatccgg ccagcttacg ggagcggccg ggggacgcaa
      661 cgggtgggat cgacgccgcc gggcgggcga aggtgcaccg gccgcgaggc tcgggtataa
      721 gcggcgcgcg atggccacga cgacggcgcc ggtccctctc gattacgtca agctgttcac
      781 cgatggcgcg tgcaagggca accccggtcc gggcggatgg gcctttatcc tgcaacaccc
      841 ggcgagcggg aaggcgcacg aggggtcggg cggcgaggcg cacacgacca acaaccgcat
      901 ggagctgatg gcggtgatcc gcgggctcga gatgttgaga ttaccgctga cggtcgacct
      961 gttctcggac tcgcagtatg tgctcaaggg tatgcgcgag tggatggccg ggtggaaacg
     1021 ctcgggctgg cgcacggcag accgccagcc ggtcaagaat caggacctgt ggatggcgct
     1081 ggacgagctg cagcagaagc acacgctgcg cttccactgg gtgaagggcc actcggggca
     1141 cccggagaac gagcgctgtg atcgcatggc cgtggcagcc cgcaacctcg cgggccgcgg
     1201 gccgtaacag tcacttcggg gtcggctcga gctcgacggg cttcttcggg tcggggcgct
     1261 tcgggcccgc gggggccgac ggcggcgcgc tcgcggagcc tggctgtggg ccagtcgtcg
     1321 gggcgcccgt gggggcagtg gtgggcacgg cggccgggcg ggacggggtc gggggcgggc
     1381 gcaggcggcc cttgccgggc acctcgatgt gggcgagcgc gggcaggtcg cgcatgaagc
     1441 cgacggtaaa ttcgagcttt tgtttggggt cgacgccctc gcgcttgctc ggctcctcga
     1501 cgaccttgcc gggcttgaag ctgtgccaca acgagccctc gccgagccgc aggtcggcga
     1561 gcgcggcgct catttgctcg agcgcctcgg cggtgggggc ggtgatcgtg aagcggcagc
     1621 ggatcgtgtg gcactcgctg gcggcgacgg agatcgcagg gccgtcggcg gcgaacaccg
     1681 cctggcgggt ggcgctgacg atctggctga tcaacggctt gtgggcggtc tgccaggcct
     1741 cgaaggtcgg ctccttgctg aagccgatcg gctcgtaata agcccacagc tgctcgagca
     1801 ggtagcgctc gcgcgggcgc gagaagggct cgtaggcgag ctcgcgcggg ccggtgcgct
     1861 cgggcttggt gaccttgccg gccttcttgt cggcctcctt ctgcttcttc tcgtcgtcct
     1921 ttttcttctt ctcggcggcc ttcttcttct cttcgagctc ggcggggctc ggcggcggca
     1981 gcgggccggg cgggacgtgc atcgcgtagt gcaggccgaa ggcggccgcg ccgagggcga
     2041 cgggcagcag ccaggtgcgc agggtgacga ggagggcggc gcggcgcgaa cggccaggga
     2101 gcggggtgcc ggaggtcatg ggacagatgg gacagacggg acggatggga cagatggaac
     2161 agaagggatc aaagagaccg aagccggggt cgggatgcgg acgtgccagg cgccgtcctc
     2221 ttgcacgagc gtgagggtat acgactcctg ctgcgcgccg accagccgca cgtgcgcggt
     2281 cgcgccgtcg ttgtcgacca gctgggtctg cgcgagctcg aaggtcggcg agacgtcgac
     2341 gatctgcagc atctcggcgg gctcgatgcg gcggcgacca ccgacctggt cggaggcctt
     2401 gtcggcgacg gcggcgaggt ggtcggccgc ggccttctcg agcagcggca gcacggcctt
     2461 catgtcgccg cgctggaccg ccgcggtgaa ggcctgcgcg gtgcgtaccg ggtcggcgcc
     2521 ctgctcgcga caggcgcccg agagcgcggc ccaggcgatc aacagcgtgg tgcgcaggac
     2581 cgggtgcagg gcgatcgtga cagtgccctg cggggtcgcg atctcgatgc cggcgctttg
     2641 gggttggttc gcgggcacga ggccgtgctg gtcttcggga caggtcatca ctcgtcctcc
     2701 ttcacgcggt gcagcccgag gcgcttgagc agctcgcgca gataatagcg ggtgatgccg
     2761 gcagccgccg aggcgcgcga gaggttgccg tcgtgggcct cgagcaggtg ggtgaagtag
     2821 cgggtctcga agtcgtcgag cagcgcctgc ttggcctcct tgaacggcag ctcgaacagc
     2881 gccgagaggt cgcccgcggg ggtgctggcg gccggggcgg gctcggcggg ccgggcggcc
     2941 gcggggcggt gcagcagcag gtcggtcggc tcgatgatgc cgtgagtcgc gagataggtg
     3001 gcccgctcga tcgtgttctt gagctcgcgg acgttgccgg gccaggcgtg gcgcaggagc
     3061 gcctggcgag cggcggcgct gagcgccggc gccggcttgc cgggcatccg gcgcgcggtg
     3121 tcggcgatga acttctcggc gagcaggatg atgtcctcgc cgcggtcgcg cagcggcggc
     3181 agctcgacgg ccatcaccga caggcgaaag tacaggtcct cgcgaaagcc gccgtgcgag
     3241 accatgtttc gcaggtcgcg gtgggtggcg gcgacgatgc ggacatcgac cttgcgcacg
     3301 gtgctctcgc cgatcctctg gacctcgcgg cggtcgagca cgcgcaggag cttgggctgg
     3361 aggtcgagcg ggagctcgcc gatctcgtcg aggaagaggg tgccgccgct ggcctcctcg
     3421 aagcagccgg cgcggtccgc catcgcgccg gtgaacgagc ccttcttgtg gcccaggatc
     3481 gcagcctcgg cgagctcgcg gggcagggcg ccgcagtcga gcacgacgaa ggggccctgg
     3541 tggcgcgggg actcgtcgtg caatgcgcgg gcgacgagct ccttgccggc cccggtctcg
     3601 ccgccgacga gcacgctcat ctcggtgccg gcgacgcgct cgagcacggc gtagatctcg
     3661 cgcatggcgg ggctggtgcc gagcatcgcg ccgaagtggt cgcggcccga gagcgggacg
     3721 tggacctcgt cctcggcgag gaaggcgatc gcggtcttgc cgagcaccat cgtcatgccc
     3781 ggctcgaccc acgcctcctg gacgcggacg ccgtgcacga agctgccgtt ggtgctctgt
     3841 aggtcacgca gcagcacgcc gcccttgctg accagcagct cggcgtgcag gccgctgatc
     3901 gagctgtcgg tcaatacgag gtcgttgacg gcgctgcggc cgacggtcag gcgcgcgcgc
     3961 gcggcctgga tctgggcgcc cgcgtcgggg ccgtccatca cccggagcat gacgcgacgc
     4021 agcgagcgcg agtgctgggt ctgcccgaag gtggtcgtca gactcgcttc gatcggctca
     4081 tggggcttga agggcacggc gaagtcaatt caagagcggg ggcttgtcga cgaccttcaa
     4141 gtggcgcgcg cggcgacccg cgcgggcggc cactttgccc gaacccgggg gggttcgcca
     4201 gcgccaggcg agcagcaggc cgcacagcgc cgcgaccgcc gtgggcaccg ccacgggcca
     4261 attaccgcgc aggagcggcc ccagcagcac cagcgcggtc acgagcgccg cgagcccgcg
     4321 acccgagagc ggcagggcgc cgaacagttg caccggttgc cgggcatgca acacgccgaa
     4381 ggcgacgacg gccgcgatgt cggccggcag cgtgccgccc atcggtgcgg cgagcacctg
     4441 cgggacggcg aggccgagca gcgcgacggc gaggtagccg acgaacgcgg acagcagcac
     4501 gagcgtgagg tagcgcgccg taccccagcg ctcctcgacg tggccgccga tgaaccactg
     4561 gatgaacagc gtgccgatca ggccgccgag ctggccgtcc gggtagagga agatcgcggt
     4621 ggccggctgc cacagggctc gatcggtgaa gccgaggttg agggtcaggg tgccggcgag
     4681 cgggtggtcg gcgagcaggg cgctggccgc cgcggcgatg atgagcagcc agaagccgcc
     4741 gcggtgaaac cggggcaggc tggcgaggac ggaggagcgg gccacggggg gccagtgtag
     4801 cagccatcac gcgcggaggc gggcgcggag cgcacaggcg ggcgggcccc ggcgcgggcc
     4861 gattttttgc gatccgggcg atggatctcc aaggtgggcc catgagctcc aagaaccgtc
     4921 gccagcaccg gatgttcgag accctccaca ccgagtacca cctgcgcctc gacgagtgcg
     4981 tgggcgtgcg cgacaagcac tccggcgagt ggtaccgcga ccacccggcc ctgcgcctgc
     5041 gtgcgatcca ggtgccgtcg gtcggggcgg acgaggagtt catcggccgg agattgcagt
     5101 tctggggcga gttcgacgac gtgacgacct cgccggtcgt cggcgtcgac cgcccgcacc
     5161 gcgagtcgct gtacggttac gtcagcttcg ctcgctcggg tgagatcgcg gcctgacatc
     5221 tcgccgacgc gaactcgtca cacgcgcgtg gcttgcccga cgagggcccg ggggtaacct
     5281 cgcggcatgt cagcaggcac ggtttgcccg caatgtcccg gtagcgcgct gtccccgatg
     5341 gagctggatc gcggccggct tcatcgctgc ttcgattgcc gcggcgtctg gctggagcgc
     5401 gacgccgcgg tggcgatgtt cgggggacgc ccctgtcact tcgaaccgcc ggcgtcggac
     5461 ggagcgcagt gcagcgtgtg tcgcggcgcg atggcgggcc tgtacttcat cggcgaggcg
     5521 ccggtgcgcg tgtgtggatc tcacggcgta tggctcggcc cgaccgaccg cgtgctgctg
     5581 ccagagccgc tgcagcgcaa gctcaagcgt cgtgacttcg tcgatgagct gctcggctgg
     5641 ttcgagtccg acgacgtgag cagtggcccg ggggccgggg ggtcgagcga gtacttcgag
     5701 gtctcggcgt cggacggtgg tggcgacggc ggggccagct gcgggggctg tggcggctgc
     5761 ggcggcggtg actagtcgcg gtgctcggtg ccgatgcggt agctggcttt tgggacaggt
     5821 cgagcgcgcg aacgttcaaa ggcgccgcgg cgcgtgttca ccccgcaggg ggtaggaaca
     5881 cgaccgggcg ccgttgtcgg tccggggtcc gtgatcggcc gcgctgctcg ttcgtcgaac
     5941 agcgcggggg cgcgaggtta ctcggccttc ttggcggcgc ccttggtggt ggcgcccttg
     6001 gtggcgtcgc cggtcttggt ggcgcccttg ctcgggtcgg cggtcttcgt ggtcgagggc
     6061 gtcgtgctcg acggggtggt cgagggggca gaggcggtgg tggcgggggc ggcggacggg
     6121 tcggccgtcg gggtgccggc ggcggcggtg tcggccgtcg gg
//
LOCUS       DASWNJ010000201         5496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_463280, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000201 DASWNJ010000000
VERSION     DASWNJ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463280"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1939
                     /locus_tag="VGB85_03760"
     CDS             <1..1939
                     /locus_tag="VGB85_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133163.1"
                     /translation="VRDGRVRDVERVFRPVELAFTLGSDGTVDVDGPTLSLAARAVVG
                     NFDPAVLALQERRTAPVTARLDFAAWRAQVTRVVPDESFTGEALAQIERARREVVLHA
                     SWLTLGDVRAALDAAFLRGVHVVVAARDAPHLVFSDQSGGPVLEIGAMAVGPLPCALI
                     VDAATGLVIADVALRWRDEPLHGVVVGEVNGPFAVQHRRLLVDSVLDFLPDVAPGGFA
                     APSGLLYEWTVIAHLARLYLAPTPAHALAVKAAFGRRVTGLEGVEALHTLSTIGRKLA
                     PGLAADSWDWRPAWDKVLRVLLVRDELPVEELHRLLKAAPPTVTASHFVDTAVDAYAS
                     GIGDRQRRTLRQIQAVAVRRWGRGITTTCPAWVRARDRNLAAVDLSGPTLRNLADDAA
                     DLLPPSEAAAWAIGVLARLNPQHGFVAWVTTAQALRPLVGTAWDNHSAAAWRVWLETA
                     PPGVDAALSIALEVIALEAVAAPLLPRGASPAQVVALRRRVIAAWPAAAAAPYWATTL
                     RSALPALGPAYRVTTHGPAVRALVADVADWPEGQATVTTWAAGIADAMRHADDLPALV
                     YWLAELAGLAPALAGGLLPRARNAVHPHRQALRDARTRSLPLWSRISDAWRMLGLKQA
                     DLEALATTTHQQPSKKGGRKR"
     gene            1936..2772
                     /locus_tag="VGB85_03765"
     CDS             1936..2772
                     /locus_tag="VGB85_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133164.1"
                     /translation="MSISGERELELEREQMERVRLTQVRDECRALADACDAEIRQVRD
                     VAVQQLAAAALQQVAAGLALARGELPERPDAALGRLGQLQTALHRAIADGEARARTWS
                     EEQVRFVADTRVAVQRAAAVGGDTGPAARARALAEAGDLVAAREALVAADSAAANARS
                     ANLDERVRREVVRGILHSLRDLGFVSAGPQLDAGVVVLEGRLATGRRAVFEVHLDGRL
                     CFDLDGYEGRACAEDLARVDTILRDRFGVQLGPPQVVWKNPDRLTQGALHGPASGTHK
                     KA"
     gene            2777..4618
                     /locus_tag="VGB85_03770"
     CDS             2777..4618
                     /locus_tag="VGB85_03770"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0133165.1"
                     /translation="MTQPSWFTTLRREADIRGGLVLYGDTRDLFWSPEAAAYQTLPRL
                     LVGALTDFTLQATWNLVEGLQFADRAHAKRWDVIVTRGIAGPTRGAAYEVEEPAPGPT
                     APVPISLEELIARYRLVLAEPRERPLLIIDWSHLLVTQPAHPDPGERRWLLRLAQALV
                     GDSTQVLNSDALRARRGLCVLVSANLGAVPPILYQGEPRIRLLAIPAPGRDERRAFFD
                     RHFDDLQIAAEKAAPGVAGVSPRAATIDLLADATDQLTTADMRQVILLSRGVPEHLTA
                     ERLLNLYRFGDQRSPWEELSEGRLRGVRDDLRRRVLGQDAAVEAVATMIVRAWLGLAG
                     LQHSARRAKPKGTLFFVGPTGVGKTELAKACAAFLFGDEAACIRFDMSEYNHEHSDQR
                     LVGAPPGYVGFEDGGQLTNAVTARPFCVLLFDEIEKAHGRVLDKFLQILEDGRLTDGR
                     GETTWFSETVIIFTSNLGASNVPDDADPQTHEDHFRGSVEDHFVHALGRPELLNRLGD
                     NIVVFQPVADGGLRRKILDLKLTPLATHLRERHGVELHLGDDVRDHIVGLSRPQQGGR
                     GLLNAIERVLMNPLSHFLFERVQQLRRGRIVTVELVDGNLDFDLSDA"
     gene            4611..5210
                     /locus_tag="VGB85_03775"
     CDS             4611..5210
                     /locus_tag="VGB85_03775"
                     /inference="COORDINATES: protein motif:HMM:NF024747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S single cluster domain-containing protein"
                     /protein_id="HEY0133166.1"
                     /translation="MPDNLLNVAAVRPRTRVNGPGWRAAVWVQGCTIRCPGCFNPDTH
                     PHVARQLWEPERLADALIRDDIEGISILGGEPFEQAAACAVLAHRVQQAGKSVVTYSG
                     YTADNLRAAALPAVHHLLAATDLLIAGPYIAASRNDGRGWHGSTNQEFVFLTDRYNLD
                     VLRAADHLPVVEIRIDGTHIDWTGIPGPADLRYLRGLNR"
BASE COUNT          797 a         2077 c         1748 g          874 t
ORIGIN      
        1 cgtccgcgat ggccgggtcc gcgatgtcga gcgagtgttc cgcccggtcg agcttgcgtt
       61 caccctcggt tccgacggga ccgtcgacgt cgacggcccc accctcagcc tcgcagcgcg
      121 cgcagtcgtc ggcaacttcg atccggcggt cctcgctctt caggagcgcc ggacggcgcc
      181 agtgactgca cgcctggact tcgctgcctg gcgggcgcag gtcacccgcg tcgtccccga
      241 cgagtcattc accggcgagg cgctcgcgca gatcgaacga gctcgacgcg aagtggtcct
      301 gcacgcgtcc tggctcaccc tcggcgatgt ccgcgccgcg ctcgatgccg cgttcctgcg
      361 tggcgttcac gtcgtggtcg cggccagaga tgcgccacac ctggtcttct cggatcaaag
      421 cggtggcccg gtgctcgaga ttggcgcgat ggcggtgggg ccccttccgt gcgcgctgat
      481 cgtcgatgcc gcgaccggcc tcgtaatcgc cgacgtcgcg ctgcgctggc gcgacgagcc
      541 gcttcacggt gtcgtggtcg gcgaagtcaa cggcccgttc gccgtccagc accgccgcct
      601 cctcgtcgac tccgtgctcg acttcctccc cgacgtcgcg cccggtggct tcgctgcgcc
      661 gtccggtctg ctgtacgagt ggaccgtcat cgcccacctc gcccgtctct acctcgcgcc
      721 gactccggcg catgctctcg ccgtaaaagc tgccttcggt cgccgggtga cgggcctcga
      781 gggcgtggag gcccttcaca ctctgagcac catcggccgc aagctcgcac ccgggctcgc
      841 ggcagactcc tgggactggc ggccggcgtg ggacaaagtg ctccgcgtgc tgctcgtccg
      901 cgacgagctg cccgtcgagg agctacatcg tctcctgaag gctgccccgc cgacggtgac
      961 ggccagccac ttcgttgata ccgccgttga cgcatatgcg tcgggtatcg gtgaccggca
     1021 acgacgcact ctccggcaga tccaggcggt cgcagtacga cggtggggtc gggggatcac
     1081 cacaacctgt cccgcgtggg tccgcgcccg cgatcgcaac ctcgccgccg tcgacctctc
     1141 cgggcccacg ctacgcaatc tcgccgacga tgccgcagac ctgctccccc cgtcagaagc
     1201 tgctgcgtgg gcgatcggcg tgctcgcgcg tttgaacccg cagcacggct ttgtcgcctg
     1261 ggtgacgaca gcacaggccc tacgccccct cgtcggcacc gcatgggata accactcggc
     1321 cgccgcctgg cgcgtctggc tcgagacggc gccgcccggt gtggatgccg cgctttccat
     1381 cgcgctggag gtcatcgcgc tcgaagcagt cgcggcgccg ctcctccccc ggggcgcctc
     1441 accggcgcag gtcgtcgcgc tgcgccggcg cgtcatagcc gcctggccgg cggctgctgc
     1501 cgccccgtac tgggcgacga cgctgcgctc ggctctccca gcgctcggcc cggcgtaccg
     1561 cgtgaccacg catggtcccg ccgttcgcgc tctggttgct gacgttgccg actggcccga
     1621 agggcaggcg accgtgacca cctgggccgc cgggatcgcc gacgccatgc gccacgcgga
     1681 cgacctcccg gccctggtgt actggctcgc cgagctcgcg ggcctcgccc cggcgctcgc
     1741 cggagggctc ctcccgcggg cccgaaatgc ggtccatccc caccgccagg ccctgcgcga
     1801 tgctcgtacg cggtctcttc cgctatggag ccggatctcc gacgcctggc gtatgctcgg
     1861 gctcaagcag gcggacctcg aggcgcttgc aacgacgacg catcagcagc ccagcaaaaa
     1921 gggaggacga aagcgatgag catcagcgga gagcgcgagc tcgagctcga gcgtgagcag
     1981 atggagcggg tgcgtctgac ccaggtacgc gacgagtgcc gcgccctcgc tgatgcctgc
     2041 gacgcggaga tccgacaggt ccgcgacgtc gccgtccagc agctcgccgc cgccgcgctg
     2101 caacaggtcg ctgccggcct cgctttggcc cgaggcgagc ttcccgagcg ccctgacgca
     2161 gcccttggcc ggctcggaca gcttcagacg gcgctccacc gagcaatcgc cgacggcgag
     2221 gcccgcgccc gcacctggtc ggaggagcag gttcgcttcg tcgccgacac ccgcgtcgcc
     2281 gtgcagcggg ccgccgcggt tggtggcgac acaggacctg ccgcccgcgc ccgcgccctc
     2341 gccgaggccg gagacctcgt cgccgcgcgc gaagccctgg tcgctgccga cagcgccgct
     2401 gcgaatgctc gctctgcgaa cctcgacgag cgagtccgcc gcgaggtcgt gcgcggcatc
     2461 ctccattcgc tgcgcgacct cggcttcgtg tcggccggcc cgcagctcga cgccggcgtg
     2521 gtcgtgctcg agggccgcct cgccactgga cgccgcgccg tattcgaggt tcacctggac
     2581 ggtcgcctgt gcttcgacct ggacggctac gagggccgcg cgtgcgccga ggacctcgcc
     2641 cgcgtggaca cgatcctacg cgatcgtttc ggcgtccagc tcggcccccc gcaggtcgtg
     2701 tggaagaacc ccgaccgtct gactcagggg gcgcttcatg gccccgcgag cggcacccac
     2761 aagaaggcat aaggacatga cgcagccctc ctggttcacg accctgcgac gtgaggccga
     2821 catccgcggc ggactcgtgt tgtatggcga cacccgcgat ctgttctggt ccccggaggc
     2881 tgccgcctac cagacgctcc cccggctcct cgtcggcgcg ctgacggact tcacgctgca
     2941 ggccacgtgg aacctggtgg agggcctgca gttcgccgac cgggcgcacg cgaagcgctg
     3001 ggacgtcatc gtcacgcggg ggatcgctgg cccgactcgg ggcgcggcct acgaggtcga
     3061 ggagcccgcg cccggaccca cggcccccgt cccgatcagc ctcgaggagc tgatcgctcg
     3121 ctaccgcctt gtcctggccg agccgcgcga gcgtccgttg ctcatcatcg attggtcgca
     3181 cctgctcgtc actcagcccg cacaccccga ccctggcgag cgtcgctggc tcctacggct
     3241 cgcccaggcg ctcgtcggcg actcgacgca ggtgctaaac agcgacgccc tgcgggcccg
     3301 ccgcgggctc tgcgtgctcg tgagcgccaa cctcggggcc gtcccaccga tcctctatca
     3361 gggcgagcca cggatccgct tgctggccat cccggcgcct gggcgcgacg agcggcgcgc
     3421 attcttcgac cgccacttcg acgatctcca aattgcagcg gagaaggctg cgcccggcgt
     3481 ggccggtgta agtccgcgcg ctgccacgat cgacctcctt gcagatgcca ccgaccagct
     3541 caccaccgcg gacatgcgcc aggtgatcct cctgtcgcgc ggggtgcctg agcatctgac
     3601 cgccgagcgt ctgctcaacc tctaccgctt cggcgaccag cgctctccat gggaggagct
     3661 cagcgagggc cgcctgcgcg gcgtccgtga cgacctgcgc cgccgcgtgc tcggccagga
     3721 cgccgccgtc gaagccgtgg ctaccatgat cgtccgcgcc tggctcggtc tcgcgggatt
     3781 acagcattcg gcgcggcgcg cgaagccgaa gggtaccctc ttcttcgtcg ggccgaccgg
     3841 cgtcggcaag accgaactcg ccaaggcatg cgccgcattt ctgttcggtg acgaagccgc
     3901 gtgcattcgc ttcgacatga gcgagtataa tcatgagcac agcgaccagc gcctcgtcgg
     3961 cgccccgccg ggctatgttg gcttcgagga cggcggccag ctcaccaacg cggtcaccgc
     4021 tcgcccattc tgcgtcctcc tgttcgatga gatcgagaag gcccacgggc gcgtcctcga
     4081 caagttcctg cagatcctcg aagacggccg cctcaccgat ggacgcggag aaaccacctg
     4141 gttctcggag accgtcatca tctttactag caatctcggc gcctcgaacg tccccgacga
     4201 cgccgacccg caaacccatg aggaccattt ccgcggcagc gtcgaggacc acttcgtcca
     4261 cgccctcggc cgccccgagc tgctcaaccg cctcggggac aacatcgtcg tgttccaacc
     4321 cgtcgccgat ggcggcctgc gccgcaagat cctcgacctc aagcttactc cactcgctac
     4381 ccacctccgc gaacgtcacg gggtcgagct gcacctcggc gatgacgtac gcgatcacat
     4441 cgtcggcctc tcccgacccc agcaaggcgg tcgcggcctg ctcaacgcca tcgagcgcgt
     4501 cctgatgaac cccctgtctc acttcctgtt cgagcgcgtc caacagctcc ggcgcgggcg
     4561 catcgtcacc gtcgagcttg tggacggcaa cctcgacttc gacctgagcg atgcctgaca
     4621 acctcctcaa cgtcgccgcg gtccgcccgc gcacccgtgt aaacggcccc ggatggcgcg
     4681 ccgctgtctg ggtccagggc tgcacgatcc gctgccccgg ctgcttcaac cccgacaccc
     4741 accctcacgt cgctcgacag ctctgggagc ccgaacgact cgccgacgcc ctgatccgtg
     4801 atgatatcga gggtatctcc atcctcggcg gtgagccatt cgagcaggct gccgcatgcg
     4861 ccgtgctcgc tcaccgcgtg cagcaggcgg gcaaatccgt cgttacctac agcggctata
     4921 ccgcagacaa cctgcgagcc gccgcgctac ccgccgttca ccatctcctg gccgccaccg
     4981 acctgctgat cgccggcccc tacatcgccg ccagccgcaa tgacggccgg ggctggcacg
     5041 gcagcaccaa ccaggagttc gtgttcctca cggatcgtta caacctggac gtccttcgcg
     5101 cggccgatca cttacccgtg gttgagatcc gaatcgacgg cacgcatatc gactggacgg
     5161 gtatcccggg ccccgccgat cttcgctacc ttcgtggcct caatcggtga ggcctgaggt
     5221 agcccctcgg gagagaccgg acgcggccgc ccatgcccgc cccctgcctt caatcgccat
     5281 aaaccttcga gctcctgacg gcctcctcga tccgggccga gaccgtctcg gggatcgcgc
     5341 gttggtggcg caagggtcgc agtcgagcga gacagagcac cgaaggacca gttggaatcc
     5401 gtgactcggc gcgagaacgc gcaaatcaag tgctcaaatt ggaccgcccg tgcgttgaag
     5461 ccgcgagcac tgagttcgcc gcatataggc gcatcg
//
LOCUS       DASWNJ010000202         2098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_463329, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000202 DASWNJ010000000
VERSION     DASWNJ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463329"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VGB85_03780"
     CDS             <1..586
                     /locus_tag="VGB85_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133167.1"
                     /translation="RLEVPGTTAALLRSAVTLQPALRWLVPILAALGLALGLALRAPP
                     LRARLDAVALRLPGLGRSLHVHGGLRLTRLLALLATVQLPLPAALELAAPRLGNAALG
                     LALLRARTHVAQGVDPGTAVIDAGLLPPIAAELLRGVGPDARATALAALVRLCEAEAD
                     EHARRIDRVTRAVQATVAALLVAGLWLVLGPLQP"
     gene            complement(593..>2098)
                     /locus_tag="VGB85_03785"
     CDS             complement(593..>2098)
                     /locus_tag="VGB85_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133168.1"
                     /translation="APLRMLNAVIIFSNTGKADMTKAAFMDAVKADYNVTLPADLRSP
                     DLQKLLDKARKDSGATAPTEALGHTPPAIDDVCGKDIVFNALARQVPDGGQVVLYWRQ
                     AGGGEFKTSTMDTFGNYATVTLTANDHGDKPVDYFFYVFDSGNKDLGNRGDQENPLKL
                     EIACQKEEAPKPKAVEDEPKAKSGLPRVFINLGLGTGAGIARGVADQSYKQLSPVDGY
                     SPADYACSIARWQAGSEDVPADSTGFATALGNLSPAQYGPYTAAELTTAYNADTCAQH
                     HPVKSGFASAPFHVAPEIGFRIGRSLVLSVFGRLQLVTGSKVYRDDPTLDLASSYNNE
                     VRSPNPEGVRTKPGFTGAGGLKIKYFLGKDESKFRLFVGGVVGGGFARLRVPMGFAND
                     RNGNSVPDDREVPAVNIGACQPVWPYGGACDGATAQSEATLAGSVVAGADKAQRVDTV
                     RVGGGFIGVTFGFNYQVAKHFALFAEIQPTVWFPKTTSFMLDFTLGPVITF"
BASE COUNT          250 a          731 c          783 g          334 t
ORIGIN      
        1 tcgcctcgag gtgccgggca ccacggcggc gctgctgcgc tccgccgtca cgctgcaacc
       61 ggcgctgcgc tggctcgtgc cgatcctcgc ggcgctgggc ctcgccctgg gcctggccct
      121 gcgggcgccg ccgctgcgcg cccggctcga cgcggtcgcg ctgcgactgc ccggcctcgg
      181 tcgctcgctg catgtgcacg gcggcctgcg tctgacccgg ctgctcgcgc tgctggcgac
      241 ggttcagttg ccgctgccgg ccgcgctcga gctcgccgcg ccgcgcctcg ggaacgcggc
      301 gctcgggctc gcgctgctgc gggcgcgcac ccacgtcgcg cagggcgtcg atcccgggac
      361 cgcggtgatc gacgccggcc tgctcccgcc gatcgccgcc gagctgctcc gcggcgtcgg
      421 tccggacgcg cgtgcgactg ccctcgccgc gctcgtgcgg ctgtgcgagg ccgaggccga
      481 cgagcacgcg cgacgcatcg accgggtcac gcgcgccgtc caggcgacgg tcgcggcgct
      541 gctcgtcgcg ggcctctggc tcgtgctcgg gccgctacag ccctgagcag ggctagaacg
      601 tgatgaccgg cccgagggtg aagtcgagca tgaagctcgt cgtcttcggg aaccacacgg
      661 tcggctggat ctcggcgaac agcgcgaagt gcttggcgac ctgatagttg aagccgaagg
      721 tcacgccgat gaagccgccg ccgacgcgca cggtgtcgac gcgctgggcc ttgtcggcgc
      781 ccgcgacgac cgagcccgcc agcgtggcct ccgactgcgc ggtcgccccg tcacacgccc
      841 cgccgtacgg ccacaccggc tggcaggcgc cgatgttgac cgccgggacc tcgcggtcgt
      901 cgggcacgct gttgccgttg cggtcgttgg cgaagcccat cggcacgcgc aggcgggcga
      961 agccgccgcc gacgacgccg ccgacgaaca ggcggaactt gctctcgtcc ttgccgagga
     1021 agtacttgat cttgaggccg ccggcgccgg tgaagccggg cttggtgcgc acgccctccg
     1081 ggttggggct gcggacctcg ttgttgtagg agctggcgag gtcgagggtc gggtcgtcgc
     1141 ggtagacctt ggagccggtg acgagctgga ggcgtccgaa caccgacagc acgagcgagc
     1201 ggccgatgcg gaacccgatc tccggggcga cgtggaacgg cgcggaggcg aagcccgact
     1261 tgaccgggtg gtgctgggcg caggtgtcgg cgttgtacgc ggtggtcagc tcggccgcgg
     1321 tgtacgggcc gtactgggcc gggctgaggt tgccgagcgc ggtggcgaag ccggtcgagt
     1381 ccgccggcac gtcctcgctg ccggcctgcc agcgggcgat cgaacacgcg tagtcggccg
     1441 ggctgtagcc gtcgaccggc gaaagctgct tgtagctctg gtcggcgacg ccgcgcgcga
     1501 tgccggcgcc ggtgccgagg ccgaggttga taaagacgcg cggcagcccg ctcttggcct
     1561 tgggctcgtc ctcgaccgcc ttgggcttgg gcgcctcctc cttctggcag gcgatctcga
     1621 gcttgagcgg gttctcctgg tcgccgcggt tgccgaggtc cttgttgccg ctgtcgaaga
     1681 cgtagaagaa gtaatcgacc ggcttgtcgc cgtggtcgtt cgccgtcagg gtcaccgtcg
     1741 cgtagttgcc gaaggtgtcc atcgtcgagg tcttgaactc gccgccaccg gcctgacgcc
     1801 agtacagcac gacctggccg ccgtccggca cctgacgcgc cagcgcgttg aacacgatgt
     1861 ccttgccgca cacgtcgtcg atcgccggcg gagtgtggcc gagcgcctcg gtgggcgcgg
     1921 tcgcgccgga gtccttgcgc gccttgtcga gcagcttctg caggtccggc gagcgcaggt
     1981 ccgccggcag cgtgacgttg tagtcggcct tgacggcgtc catgaaggcc gccttcgtca
     2041 tgtcggcctt gccggtgttc gagaagatga tcaccgcgtt gagcatccgc agcggcgc
//
LOCUS       DASWNJ010000203         2683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_286413, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000203 DASWNJ010000000
VERSION     DASWNJ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286413"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1777
                     /locus_tag="VGB85_03790"
     CDS             <1..1777
                     /locus_tag="VGB85_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133169.1"
                     /translation="SGTTGGSDTTSSGTTGEPGSTSSGTTGEPGSTDTSSDTTSSDTT
                     SSDTTGSGTTGEPGTTGEPGTTGASGTTGDSDSEGEGEDAWCCEPGCSIPLMQTHIGK
                     VAVQHDGTLITTYDQAKRLRLWVASVLAEILVVDDVDEHLLVGGTLVYEKAGQLHIVD
                     AWNAVELAACPTDTQWGVARDGGYVWTAGAAGVELLELDCSLRWSAAGSLADAKILAT
                     ADALHVYDADLGAQTVTHFDATDGSASEAEFLGTFASWFADVPRYWATQGPAYRVYDL
                     DNSLVKFTTGLPKYGWGTRLAGNGTMYDLFDPADKANYDPTTHEYSGGAILSSEQDEV
                     RLIRMDVDPPTEQVVKPVCCVNDFSGWDFAFSGGAWVVGGVNGYVADHLGKFVSDPQA
                     LYLEGGQAGRVAVGLADARVYLYDLTDDCELTALPMFKRKGGGTMRMSGNGALLVSSE
                     GYYYQNNQGWFAASRLYTLPDGVVIGEYPVGLHSDGLGDHDFADDGMYWSRLKSNAYY
                     TAEIYKTLITGWGYNQSNVLPRFSPDSGHYATSDGSHWVWSWAEAHTTIYATTRASWS
                     VSPRASCTASSTRSTCWSAVTPMPT"
     gene            1717..2043
                     /locus_tag="VGB85_03795"
     CDS             1717..2043
                     /locus_tag="VGB85_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133170.1"
                     /translation="MHGFIDEEHLLVGRYAYADLKSYFIAMDIVALDGTVVQTTNLPE
                     IRRVMHIETGEILVEIHPTKQGAIYNAFTGEKLWTAPPKARVAVVGPDHIATSDAAGV
                     QIIKWR"
     gene            complement(2056..>2683)
                     /locus_tag="VGB85_03800"
     CDS             complement(2056..>2683)
                     /locus_tag="VGB85_03800"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HEY0133171.1"
                     /translation="VVAAVRAGVRRIVRCSVLGADTPDELVEGAWHNAGDEAVRRSGI
                     AYTILRPNQYFQNFSNGRAAQTVRDQGALYLPLENCAVSNIDTRDIAAIAAEVALAPG
                     DEHHGREYDLTGGSAQTMAEVAAALGEALGKPVQYVAVPEAPVRESLLGAGLPPAVAD
                     AILGWFAYCRAGKAARVTDDARRLLGREPIGVQQFARDHVGSYQGAAT"
BASE COUNT          440 a          969 c          892 g          382 t
ORIGIN      
        1 ctccgggacc accggcggat ccgacacgac cagctctggg accaccggcg agccgggaag
       61 caccagctcc gggaccaccg gcgagccggg aagcaccgat accagctccg acaccaccag
      121 ctccgacacc accagctccg acaccactgg ctctggcacc accggcgagc ccggcaccac
      181 cggcgagccc ggcaccaccg gcgcttccgg caccaccggc gattccgaca gcgagggcga
      241 gggcgaggac gcgtggtgtt gtgagccggg gtgcagcatc ccgctgatgc agacacacat
      301 cggaaaagtc gcggtgcagc acgacggcac gctgatcacg acatacgacc aggccaagcg
      361 cctgcgcctg tgggtcgcca gcgtgctcgc ggagatcctc gtggtcgacg acgtcgacga
      421 gcacctgctc gtcggtggca ccctggtcta cgagaaggcg ggtcagctgc acatcgtcga
      481 cgcctggaac gccgtcgagc tcgccgcatg ccccaccgac acccagtggg gcgtcgcgcg
      541 cgacggcggc tacgtgtgga ccgccggggc agccggcgtc gagctgctcg agctcgactg
      601 cagcctgcgc tggagcgccg ccggttcgct cgccgacgcg aagatcctcg ccaccgccga
      661 cgcgctgcac gtatacgacg ccgacctcgg cgcgcagacg gtgacccact tcgacgccac
      721 cgacggcagc gcgagcgagg ccgagttcct cggcaccttc gccagctggt tcgccgacgt
      781 gccgcgctac tgggccacgc agggtccggc ctatcgcgtc tacgacctcg acaattcgct
      841 cgtgaagttc accaccggcc tccccaaata cgggtggggc acgcggctcg ccggcaacgg
      901 tacgatgtac gacctcttcg accccgccga caaggcgaac tacgatccga cgacccacga
      961 atactcgggc ggcgcgatcc tctcgtccga gcaggacgag gtgcggctga tccgcatgga
     1021 cgtcgacccg ccgaccgagc aagtcgtcaa gccggtctgc tgcgtcaacg acttctccgg
     1081 ctgggacttc gccttctcgg gcggcgcgtg ggtcgtcggc ggggtcaacg gctacgtcgc
     1141 cgaccacctc ggcaagttcg tcagcgaccc ccaggcgctc tacctcgaag gtggccaggc
     1201 cggacgcgtc gccgtcggcc tcgccgacgc gcgcgtgtac ctgtacgacc tgacggacga
     1261 ctgcgagctg acggcgctcc ccatgttcaa gcgcaagggc ggcggcacca tgcgaatgtc
     1321 gggcaacggc gcgctgctgg tgtcctccga gggctactat taccaaaaca accagggctg
     1381 gtttgcggcc tcgcgcctct acacgctgcc ggacggggtc gtgatcggcg agtacccggt
     1441 cgggctgcat tcggacgggc tcggggatca cgactttgcc gacgatggga tgtattggtc
     1501 gcggctcaag tcgaacgcgt attatacggc cgagatctac aagacgttga tcaccggctg
     1561 ggggtataac cagagcaacg tgctgccgcg ctttagcccg gacagcggcc attatgcgac
     1621 gtcggacggc agccactggg tctggagctg ggccgaggcg cacaccacga tctacgcgac
     1681 aacccgagca agctggtcgg tgtcgccgag ggcgtcgtgc acggcttcat cgacgaggag
     1741 cacctgctgg tcggccgtta cgcctatgcc gacttgaaga gctatttcat cgccatggac
     1801 atcgtcgccc tcgacggcac cgtcgttcag acgacgaacc tgcccgagat ccggcgggtg
     1861 atgcacatcg agaccggcga gatcctcgtc gagatccacc cgaccaagca gggcgcgatc
     1921 tacaacgcgt tcaccggcga gaagctgtgg accgccccgc ccaaggcccg ggtcgccgtc
     1981 gtcggacccg accacatcgc cacgagcgac gcggccggcg tccagatcat caagtggcgc
     2041 tgatcggcga gcgcctcacg tcgccgcgcc ctgataactg ccgacgtggt cgcgggcgaa
     2101 ctgctgcacg ccgatcggct cgcggccgag caggcgccgc gcgtcgtcgg tgacgcgcgc
     2161 ggccttgccg gccctgcagt acgcgaacca cccgaggatc gcgtcggcca ccgccggcgg
     2221 caacccggcg ccgagcaggc tctcccgcac cggcgcctcg gggaccgcga cgtactgcac
     2281 cggcttgccg agcgcctcgc cgagcgccgc ggcgacctcg gccatcgtct gcgccgagcc
     2341 gccggtcagg tcgtattcgc ggccgtggtg ctcgtccccg ggcgccagag ccacttcggc
     2401 cgcgatcgcg gcgatgtccc gggtgtcgat attgctgacc gcgcaatttt cgagcggcag
     2461 atacagcgcc ccctggtcgc gcaccgtctg ggccgcgcgg ccgttgctga agttctgaaa
     2521 gtactgattg ggccggagga tcgtgtaggc gatgccgctg cggcgcacgg cctcgtcgcc
     2581 ggcgttgtgc cacgcgccct cgacgagctc gtccggtgtg tcggccccga gcacgctgca
     2641 gcggacgatc cgccgcacgc ccgcgcgcac ggccgccacg acg
//
LOCUS       DASWNJ010000204         4264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_39267, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000204 DASWNJ010000000
VERSION     DASWNJ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39267"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VGB85_03805"
     CDS             <1..439
                     /locus_tag="VGB85_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0133172.1"
                     /translation="HISSIGGQIALPFQGLYCASKFALEGLSEALSHELHGSGVRVVL
                     VEPGDFRTGFTDNRKLVRAAGPGSAYQRQFDRTLGVFIADEQRGPPPIDVARVVEHAL
                     AVARPRLRYVVGMPLQRWSIALKKLLPWRVFAAVFRKIYRIEG"
     gene            complement(440..1693)
                     /locus_tag="VGB85_03810"
     CDS             complement(440..1693)
                     /locus_tag="VGB85_03810"
                     /inference="COORDINATES: protein motif:HMM:NF015368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133173.1"
                     /translation="MTLHPLVTLALSCLALTACPGGGSESGETGDPSSTGAPASPDTG
                     SSGATPTTGDDPTATGDTSSTGEPTGVEGCDGASLLPVPADSSLAGPWPVGVKTVTVG
                     GLTVEVWYPGTPGSEVGLAARRYDIRDSLPEGEQDKVPDAKAPWQDCDCYPDVPLDTA
                     HGPYPAVIFVHGTAGYRAQSLEHVTHWASRGFVVLAADHPGLWLRDLLGQLCGQTAVP
                     QDLAGNLDTLVAAITAPAGDLEFLAGHLDGARIGMAGHSAGGNAIAKSGDVAQVLIPM
                     AAGGVMAGAALRSTLVLGAQSDQVVDYGSQQSGYASSPAPKRLVGISNAGHLAFSSLC
                     SLANADGQDFVEIAAEFEICGAQFASALFDCVDTYTPDPTNWAITNYATSAVLEETLH
                     CASTGETFAGIMAKFPEVGEVLEEK"
     gene            complement(1755..2339)
                     /locus_tag="VGB85_03815"
     CDS             complement(1755..2339)
                     /locus_tag="VGB85_03815"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY0133174.1"
                     /translation="MDHAVHSRMPATYADLVALPSHIKGEIIDGRLYTQPRPRAWHAM
                     IEGAAFGDLHACFHRGRGGPGGWWIVVEPGIELPRAPEFSPDSAGWLRSRMPRPPGPG
                     EPWRTVPDFIFEVLSPSNRHYDLTVKRRFYAEIGVQHLWFIDPDARTLMVNRLHEGRW
                     VELGVFGSQAPVRAEPFDAVELDIGEWWPTHDGA"
     gene            complement(2405..4042)
                     /locus_tag="VGB85_03820"
     CDS             complement(2405..4042)
                     /locus_tag="VGB85_03820"
                     /inference="COORDINATES: protein motif:HMM:NF014675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HEY0133175.1"
                     /translation="MQLTQPTRGAALLVCTISIACSGGGQGESTRTTGTTGTTGAGMT
                     SGSTQPQPTTSGGTTGTGDLSESTAASETSGAVDTSTGGPTQDLVTVSHPRELRAAWV
                     ASVGNINFPSKQGLSAEAQQAELSAMFDGIAQAGLNTVILQVRPECDALYASEIEPWS
                     RYLTGTQGEDPGYDPLEFAVTQAHARGLELHAWFNPYRAKASAGSKAAANHISKLLPQ
                     YAYTYGNFLWMDPGATEVQDQLVAVISDVVGRYDIDGVHFDDYFYPYPDGDPFPDSGT
                     YDAYVQGGGQLGLGDWRRDNVNRVVERVGIEVAKLRPSVRWGISPFGIYRPGIPEGIQ
                     GLDQYDAIYADPVKWMQEGWLDYLAPQLYWPSTQEPQAYGKLIEWWSSITTGGRYIFA
                     GNYLSKIGSDADWSLDELRAQIQLSRQYAPQGSQGNIYFHVAPILNNTEGFTDMIKTE
                     FYASPALTPPIAALAGDVVAPPQVVLAGTSVTLTHEDPGGLRAWVLYRGDNDVWTIER
                     IVRASEPGLELPGSGTWAISAAARSGVESAGVRIVVP"
     gene            complement(4080..>4264)
                     /locus_tag="VGB85_03825"
     CDS             complement(4080..>4264)
                     /locus_tag="VGB85_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133176.1"
                     /translation="AVDIDGIEPEVMHKHWGCRGSLVIDARVKSHHAPPLVEDPDVTR
                     RVDALAARGGPLHGLF"
BASE COUNT          624 a         1560 c         1411 g          669 t
ORIGIN      
        1 ccacatctcg agcatcggcg gccagatcgc gctgccgttt cagggcctgt actgcgccag
       61 caagttcgcg ctcgaggggc tcagcgaggc gctgtcgcat gagctgcacg gctccggcgt
      121 gcgcgtggtg ctcgtcgagc ccggcgactt tcgcaccggc ttcaccgaca accgcaagct
      181 cgtgcgcgca gctggcccgg gctccgccta tcaacggcag tttgaccgca ccctcggcgt
      241 cttcatcgcc gacgagcagc gcggcccccc gccgatcgac gtcgcccgcg tcgtcgagca
      301 cgccctcgcg gtcgctcgcc cgcgcctgcg ctacgtcgtc ggcatgccgc tgcagcgctg
      361 gtcgatcgcc ctcaagaagc tgctgccgtg gcgggtgttc gcggcggtgt tccgcaagat
      421 ctacaggatc gaaggctgac tacttctcct cgagcacctc gccgacctcc gggaacttcg
      481 ccatgatacc ggcgaatgtc tcacccgtgc tcgcgcagtg cagcgtctcc tcgagcaccg
      541 ccgaggtcgc gtagttggtg atcgcccaat ttgtcggatc gggggtgtag gtgtcgacgc
      601 agtcgaacag ggcgctggcg aactgggccc cgcagatctc gaactcggcg gcgatctcga
      661 cgaagtcctg accgtccgcg ttggccagcg agcacaggct ggagaacgcg aggtggccgg
      721 cgttgctgat cccgaccagt cgcttcggcg ccggtgacga ggcgtagccg ctctgctggc
      781 tgccgtagtc gacgacctgg tccgactgcg ccccgagcac gagcgtcgag cgcagcgccg
      841 cgccggccat cactccgccg gccgccatcg ggatcagcac ctgcgcgaca tcgccggact
      901 tggcgatcgc gttgcccccc gcgctgtgcc ccgccatgcc gatccgcgcg ccgtcgaggt
      961 gacccgcgag gaactcgagg tcccccgcgg gcgccgtgat cgcggcgacc agcgtgtcga
     1021 ggttgccggc gaggtcctgc gggaccgccg tttggccgca cagctggccg agcaggtccc
     1081 tcagccacag gcccgggtgg tcggccgcca gcacgacgaa cccgcggctg gcccagtgcg
     1141 tcacgtgctc gagcgactgc gcgcgatagc ctgctgtccc gtgcacgaag atcaccgcgg
     1201 gataggggcc gtgcgcggtg tccagcggca catccgggta acagtcgcag tcctgccacg
     1261 gagccttcgc gtccggcacc ttgtcctgct cgccctcggg cagcgagtcg cggatgtcgt
     1321 agcgccgggc cgcgagcccg acctcgctgc cgggcgtgcc cgggtaccac acctcgacgg
     1381 tcaggccgcc gaccgtcacc gtcttcacgc ccaccggcca cggccccgcg agcgagctgt
     1441 ccgcggggac aggcagcaac gaggcgccgt cgcagccctc gaccccggtc ggctcgccgg
     1501 tgctcgacgt atcgccggtg gccgtcgggt catcgccggt cgtcggcgtc gcaccgctcg
     1561 agcctgtatc gggggacgcg ggcgcgcccg tgctcgacgg atcacccgtc tcaccggact
     1621 cgctcccccc tcccgggcaa gcggtgagcg cgaggcacga cagggcgagg gtcacaagtg
     1681 gatgcagggt catcgattct ctccgggggc gctcatggta cgacgaccgg cacgccgatc
     1741 gcaggccctt gcgcctacgc accgtcgtgc gtcggccacc actcgccgat gtcgagctcg
     1801 accgcgtcga agggctcggc ccgcacgggc gcctgactgc cgaacacgcc gagctcgacc
     1861 caccggccct cgtgcaaacg attgaccatc agcgtgcgcg cgtcgggatc gatgaaccac
     1921 aggtgctgca cgccgatctc cgcgtagaag cggcgcttca cggtgaggtc gtagtggcgg
     1981 ttgctcggcg acagcacctc gaagataaag tccggcaccg tgcgccacgg ctcacccggg
     2041 cccggcggac gcggcatgcg gctgcgcagc cagccggcag agtccggcga gaactccggc
     2101 gcacgtggca gctcgatgcc cggctcgacg acgatccacc agccacccgg gcccccgcga
     2161 ccgcggtgaa agcaggcgtg caggtcgcca aaggccgcgc cctcgatcat cgcatgccag
     2221 gcgcgcggcc gcggctgcgt atacaaccgt ccatcgatga tctcaccctt gatgtgagac
     2281 gggagcgcca ccagatcggc gtaggtcgcg ggcatcctgc tgtgaacggc gtgatccatg
     2341 gcgagggcac ggtagcatcc cgcgaccgcc tcgccagtaa aaaagcgcga ccccgcgcct
     2401 ccgctcaggg aacgacgatc cgcaccccgg cgctctcgac cccgctgcgt gccgcggcgc
     2461 tgatcgccca ggtgccggag cccggcagct cgaggcccgg ctcgctcgca cgaacgatgc
     2521 gctcgatcgt ccacacgtca ttgtcaccgc gatacagcac ccacgcgcgc agccctccgg
     2581 ggtcctcgtg ggtgagcgtc acgctggtcc ccgcgagcac cacctgcggc ggcgcgacca
     2641 catcgccggc cagcgccgcg atcggcgggg tcagggccgg gctggcatag aactcggtct
     2701 tgatcatgtc cgtgaagccc tcggtattgt tgaggatcgg cgcgacatgg aagtagatgt
     2761 tgccctgcga gccttgcggc gcatattgcc ggctgagctg gatctgcgcg cgcagctcgt
     2821 cgagcgacca gtcggcgtcg ctcccgatct tgctcagata gttcccggcg aagatatagc
     2881 gcccgccggt ggtgatgctc gaccaccact cgatcagctt gccgtaggcc tgcggctcct
     2941 gggtgctcgg ccagtacagc tggggcgcca ggtagtccag ccagccctcc tgcatccatt
     3001 tcaccggatc ggcgtagatc gcatcatatt gatcgaggcc ctggatcccc tccgggatcc
     3061 ccgggcgata gatcccgaac gggctgatcc cccagcgcac gctcgggcgc agctttgcga
     3121 cctcgatccc gacccgctcg accacacggt tgacgttgtc acgccgccag tcgccgagcc
     3181 cgagctgccc gcccccctgc acatatgcat cgtaggtgcc gctgtcgggg aaggggtcgc
     3241 cgtcgggata cggataaaag taatcgtcga agtgcacgcc gtcgatgtca taacggccga
     3301 cgacgtcgct gatcaccgcg acgagctggt cctggacctc ggtcgcgccc gggtccatcc
     3361 acaggaaatt cccataagta taggcatact gcggcagcag cttcgagatg tggttggcgg
     3421 ccgccttcga gccggcgctc gccttggcgc ggtacgggtt gaaccacgcg tgcagctcca
     3481 gcccgcgcgc gtgcgcctgc gtgaccgcga actcgagcgg gtcatagccg gggtcctccc
     3541 cctgcgtgcc ggtcagataa cgactccacg gctcgatctc cgaggcgtac agcgcgtcgc
     3601 actcgggccg cacctgtaaa atgaccgtgt tgaggcccgc ctgcgcgatg ccgtcgaaca
     3661 tcgccgacag ctcggcctgc tgcgcctccg ccgacagccc ctgcttgctc gggaagttga
     3721 tgttgccgac gctcgccacc cacgccgccc gcagctcgcg cgggtggctg accgtcacca
     3781 ggtcctgcgt cggcccgccg gtgctcgtat cgaccgcgcc gctcgtctcc gacgcggctg
     3841 tactttcgga caggtcaccg gtgcccgtcg tgcctccgct ggtggtcggc tgcggctgcg
     3901 tgctcccgga tgtcatgccc gcgccggtcg tgcccgtcgt gcccgtcgtg cgggtgctct
     3961 cgccctgccc tccgcccgag cacgcgatgg agatcgtaca cacaaggagc gccgcgcctc
     4021 gcgttggctg cgtcagttgc atcgtccctt gatagccgcc ggccacgcgc ggcacaagct
     4081 cagaacaggc cgtgcagggg tccgcctcgc gccgcgagcg cgtccactct gcgcgtcacg
     4141 tccgggtcct cgaccaaggg cggggcatga tgcgacttca cgcgcgcgtc gatcaccagg
     4201 ctgccgcggc agccccagtg cttgtgcatg acctcgggct cgatgccgtc gatgtcgacc
     4261 gcag
//
LOCUS       DASWNJ010000205         1845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_180154, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000205 DASWNJ010000000
VERSION     DASWNJ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180154"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..574)
                     /locus_tag="VGB85_03830"
     CDS             complement(29..574)
                     /locus_tag="VGB85_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133177.1"
                     /translation="MKARVAFVTSAEFSGDIGGLAGADAICVAAAMAGGLDNAGNFLA
                     YMGDGVAAPATRFVDGAKAKGFPYARRDGQKLANDLDDLFATGVRVPLVITEFGDPLP
                     ASPDPRYAWTGVGLHGEPDGEQCQEWSTTYFKYSGRVGQISPVSDSDADVFDWMIDGH
                     WVKFTSRPCATKAMHLYCFED"
     gene            complement(1458..>1845)
                     /locus_tag="VGB85_03835"
     CDS             complement(1458..>1845)
                     /locus_tag="VGB85_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133178.1"
                     /translation="EENPMSKLSPLQNVKKLYGSKDQLISKVTELLTPADGESKEDFA
                     KRLKYVANAKLLRLLQVGEKVQKLGGRTALVAKLAELKGQAKDKDFTAMLGKLSLPRL
                     LDTYQGLSRKAKTAAPAKAAAPAKKA"
BASE COUNT          276 a          630 c          593 g          346 t
ORIGIN      
        1 ggcgaggatc gggagggtgc gcatgaggtc agtcctcgaa acagtaaagg tgcatcgcct
       61 tcgtggcgca gggacgcgag gtaaatttga cccagtgccc gtcgatcatc cagtcgaaga
      121 cgtcggcgtc gctgtccgag accggggaga tctgcccgac ccgcccgctg tacttgaagt
      181 atgtcgtgct ccactcctgg cattgctcgc cgtcgggttc accatggagg ccgacgccgg
      241 tccacgcgta ccggggatcg ggcgaagcgg gcagcgggtc gccgaactcg gtgatcacca
      301 gcggcacccg caccccggtg gcgaacaggt cgtcgaggtc gttggccagc ttttgtccgt
      361 cgcggcgggc gtacggaaaa cccttggcct tggcgccgtc gacgaagcgg gtcgcggggg
      421 cggccacgcc gtcgcccatg tacgcgagga agttgccggc gttatcgagg ccccccgcca
      481 tcgccgcggc cacacaaatt gcatcggccc cggcgaggcc gccgatgtcg ccggagaact
      541 cggcgctggt gacgaaggcg acccgggcct tcattctaca gtttccggcg tcacattcga
      601 cagcgccctc gacggccggc gcgctcccgt cgcactcctc ctcggattgc agcacaccgt
      661 cgttgcacct gggtccaaaa acacagtcac cccggcatcc gccgtacaat gtgtcgttgt
      721 tggccatccc gttgtcgcac actgctcctg gcccgcccac accaggccgt cgccacaatg
      781 cgcgttttgg caggttttcg tgcaggcgcc gtcatcttta ttctcggcgt aggtcaggtc
      841 gcagccctct cctgggtcca ggtgcccgtc tccgcagtcc ccgaccgggg ccgtcgtcga
      901 gccggtcgcc gcgtccgtcg tgcccgtcgt gcccgtgctc tcgtcgccag acgccgtggt
      961 gctcgcgccc ggcccggtcg ggtccaccgc cgacgtcggc gtcggaccag ctccgccgtc
     1021 atcgtcatcg tcgtcatcgt ctccaccacc accgtcctcc gtggagctgg ccccgggcgc
     1081 atccgtatac acatgcgccg ggcatccggt cacagtcacg gtcaggccca cgagagatac
     1141 cgacaacgag cgaggcacac gccacatatg caccacgcat tacacgaccg cgcggccccg
     1201 tcaacgtgtt ttgtgggtag ttttactcgg cagatttata cgtaatatag attacctatt
     1261 tgtcatacga tgatccccgc gaaactccgc tccgacgacg cgccgcggga ggaccgaggc
     1321 cacgagcatc gcccccggcg acccgtattt cccctggtga tacgcgttgt cagcctccgc
     1381 gaaccaatga caaagggccg cgtcgctttc gcggcgcggc cccgggggct ttctacgtac
     1441 ctgccctttg ggacagctca ggccttcttg gccggcgcgg cggccttggc cggcgcggcg
     1501 gtcttggcct tgcggctgag gccctggtag gtgtcgagca ggcgcggcag ggacagcttg
     1561 ccgagcatgg cggtgaagtc cttgtccttg gcctggccct tgagctcggc gagcttggcg
     1621 accagcgcgg tgcggccacc gagcttctgg accttctcac cgacctgcag caggcgcagg
     1681 agcttggcgt tggcgacgta cttcaggcgc ttggcgaagt cttccttcga ctcgccgtcg
     1741 gcgggggtga gcagctcggt gaccttgctg atcagctggt ccttcgagcc gtacagcttc
     1801 ttgacgttct ggaggggaga gagcttggac atggggttct cctcg
//
LOCUS       DASWNJ010000206         1549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_497844, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000206 DASWNJ010000000
VERSION     DASWNJ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497844"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..841
                     /locus_tag="VGB85_03840"
     CDS             257..841
                     /locus_tag="VGB85_03840"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0133179.1"
                     /translation="MTTRIKPDEQRKQQIRTAATRCFVRRGFAATRLLDIAREAGLSK
                     GGVYFHYRAKEALFHDILEAQFAALQARWDTQQASDQPADTALRGLVLTHLQSLEQSP
                     DEARFCNLMISMASQNPEFREKFDLSLSGMRSRYAEIIRRGEFGGDPVQLAGGVIALI
                     YGVAAQSAADPQASLPLTSEQVADLAVRLVRGPA"
     gene            complement(857..>1549)
                     /gene="amrB"
                     /locus_tag="VGB85_03845"
     CDS             complement(857..>1549)
                     /gene="amrB"
                     /locus_tag="VGB85_03845"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEY0133180.1"
                     /translation="IDLIVMLGTDHHPGRLPFAITDKGYGTPLGDLRPAPELVAALER
                     RLPWIRREELRHREAISLEMGAVLLHHLYGADCPPVLPVLCGQAALLTGEDEAMTDAF
                     LATMEHVLEGRRVFWWISAELSHAGPAFGKPPLAADGVRALAERDLACLESLVAGRPE
                     QFVARCMETNETLGKPSGAAALSTMARLLPIGYRSELIDYVTAKAVGPDEGWVGLVGM
                     RFFQPAALDDDE"
BASE COUNT          234 a          561 c          543 g          211 t
ORIGIN      
        1 gacgaggtgt caaattgcgc cggccgcccc cgcgcccaaa acgcgggatc gcccgcagat
       61 cggtctactt ccgcccttta gagcactttg gctttcggcc ggcccccggg cctgctccgg
      121 gccggccccc gccaaatggg ccgtccggcc gcccacacag gcaaattgcg gcggcgtctg
      181 gacaaaccca ccggtcctcg cgctagcgtg ggggaagcgt tgggacgccg cagtggcgac
      241 gggaggtggg ggagcagtga cgacacgcat caaacctgat gagcagcgca agcagcagat
      301 ccggactgcc gcgacgcggt gcttcgttcg ccggggcttc gcggccacgc ggctcctgga
      361 catcgcccgc gaggcaggcc tcagcaaggg cggcgtctat ttccattacc gggccaagga
      421 ggccctgttc cacgacatcc tcgaggccca gttcgcggcg ctgcaagccc gctgggacac
      481 gcagcaggcg tccgaccagc cggccgacac ggcgctgcgg ggcctcgtgc tcacgcacct
      541 gcagagcctc gagcagagcc cggacgaggc ccgcttttgc aacctgatga tctcgatggc
      601 gagccagaac ccggagtttc gcgagaagtt cgacctctcg ctgtccggca tgcgctcccg
      661 ctacgcggag atcattcgtc gcggcgagtt cggcggtgac ccggtgcagc tcgccggcgg
      721 cgtcatcgcg ttgatctacg gcgtcgcggc ccagagcgcc gcggacccgc aggccagcct
      781 gccgctgacc agcgagcagg tcgccgacct cgcggtccgc ctggtccgcg gtccggcatg
      841 aacaagcgtc cgggcgtcac tcgtcgtcgt cgagggcggc gggctgaaaa aagcgcatgc
      901 cgacgaggcc gacccagccc tcgtcggggc cgaccgcctt cgcggtgacg tagtcgatca
      961 gctcgctgcg gtagccgatc ggcagcaggc gggccatcgt cgacagcgcc gcagcgccgc
     1021 tcggcttgcc gagcgtctcg ttcgtctcca tgcagcgcgc gacgaactgc tccggccgcc
     1081 cggcgacgag cgactcgagg caggccaggt cgcgctccgc cagcgcgcgc acgccgtcgg
     1141 cggccagcgg cggcttgccg aacgccggcc cggcgtggct cagctcggcg gagatccacc
     1201 agaacacgcg cctgccctcg aggacatgtt ccatcgtcgc caggaaggcg tcggtcatcg
     1261 cctcgtcctc gccggtcagc agcgcggcct gaccgcagag caccggcaac accggggggc
     1321 agtccgcgcc gtagaggtgg tgcagcagca cggcgcccat ctcgaggctg atcgcctcgc
     1381 ggtggcgcag ctcctcgcgg cggatccacg gcagccggcg ctcgagcgcg gcgaccagct
     1441 cgggcgcggg gcgcaggtcg cccagcggcg tgccgtagcc cttgtcggtg atcgcgaacg
     1501 gcaggcgacc cgggtggtga tcggtgccca gcatcacgat cagatcgat
//
LOCUS       DASWNJ010000207         1573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_463437, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000207 DASWNJ010000000
VERSION     DASWNJ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463437"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1573)
                     /gene="dnaE"
                     /locus_tag="VGB85_03850"
     CDS             complement(<1..>1573)
                     /gene="dnaE"
                     /locus_tag="VGB85_03850"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY0133181.1"
                     /translation="LNPERISMPDIDLDFDERRRGDMIRYATEKYGEERVSQIITYGT
                     IKAKQAVKDSARVLGYPFATGDKITKAMPPAVMGKDIPLAGIFDPSHKRYGEAGEFRA
                     LYESDPEAKQVVDTARGLEGLKRQWGVHAAGVILCREPLLDVIPIHRREQDGAIITQF
                     DMGACETLGLLKMDFLGLRNLTVLDDCLKHIAANRGETVVLEELDEMQSDPKTYELLA
                     KGDTLGVFQLDGGPMRSLLRSMQPDRFESISAVLALYRPGPMGVNAHNDYADRKTGRK
                     PVVPIHPELGEALEDILGDTYGLIVYQEQVMAIAQQLAGYTLGAADLLRRAMGKKKRE
                     ILDKEYIPFSDGMKANGYSEDAVKTLWDTLVPFADYAFNRAHTAGYGLVSFWTAYLKA
                     NYPAEYMAALLTSVKDDKDKRALYLAECRRMGIKVLPPDVNDSDSDFTPRGTDIRFGL
                     SAIRNVGTNVVASIVRTREAKGRFADFSDFLRKVDAVACNKKTVESLCKAGAFDSLGH
                     SRKGLVAVHAEAIDAC"
BASE COUNT          212 a          508 c          572 g          281 t
ORIGIN      
        1 gcacgcgtcg atcgcctcgg cgtggaccgc gacgagcccc ttgcggctgt ggccgagcga
       61 gtcgaacgcc ccggccttgc acagcgactc gacggtcttc ttgttgcacg ccaccgcgtc
      121 gaccttgcgc aggaagtcgg agaagtccgc gaaccggccc ttggcctccc gggtccgcac
      181 gatggaggcc acgacgttgg tgccgacgtt gcggatcgcc gacaggccga agcggatgtc
      241 ggtgccccgc ggcgtgaagt cgctgtcgga gtcgttcacg tcgggcggga gcaccttgat
      301 gcccatgcgc cggcactcgg cgaggtagag cgcgcgcttg tccttgtcgt ccttgaccga
      361 ggtgagcagc gccgccatgt actcggcggg gtagttggcc ttgaggtagg ccgtccagaa
      421 gctgacgagc ccgtagccgg cggtgtgcgc gcggttgaag gcgtagtcgg cgaacgggac
      481 gagggtgtcc cagagcgtct tgaccgcgtc ctcggagtac ccgttggcct tcatcccgtc
      541 ggagaacggg atgtactcct tgtcgaggat ctcgcgcttc ttcttgccca tcgcgcggcg
      601 cagcaggtcg gcggcgccga gcgtgtagcc ggccagctgc tgcgcgatgg ccatgacctg
      661 ctcctggtac acgatcaggc cgtacgtgtc gccgaggatg tcctcgagcg cctccccgag
      721 ctcggggtgg atggggacga ccggcttgcg gccggtcttg cggtcggcgt agtcgttgtg
      781 ggcgttgacc cccatcggac cgggccggta gagcgccagc accgccgaga tcgactcgaa
      841 gcggtcgggc tgcatcgagc gcagcagcga gcgcatcggc ccgccgtcga gctggaagac
      901 cccgagcgtg tcgcccttgg ccagcagctc gtaggtcttc gggtcgctct gcatctcgtc
      961 gagctcctcg agcacgacgg tctcgccccg gttggcggcg atgtgcttga ggcagtcgtc
     1021 gaggacggtg aggttgcgca gcccgaggaa gtccatcttc agcaggccga gcgtctcgca
     1081 ggcacccatg tcgaactggg tgatgatcgc gccgtcctgc tcgcggcggt ggatcgggat
     1141 gacgtcgagc agcggctcgc ggcacaggat gacgccggcg gcgtggacgc cccactggcg
     1201 cttgagcccc tcgaggccgc gtgcggtgtc gacgacctgc ttggcctcgg ggtcgctctc
     1261 gtagagcgcc cggaactcgc cggcctcgcc gtagcgcttg tgcgaggggt cgaagatccc
     1321 ggccagcggg atgtccttgc ccatcaccgc cggcggcatc gccttggtga tcttgtcgcc
     1381 ggtcgcgaac gggtagccca gcacgcgggc ggagtccttg acggcctgct tcgccttgat
     1441 ggtgccgtag gtgatgatct gcgagacccg ctcctcgccg tacttctccg tggcgtagcg
     1501 gatcatgtcg ccccgccggc gctcgtcgaa gtcgaggtcg atgtcgggca tcgagatgcg
     1561 ctcggggttg agg
//
LOCUS       DASWNJ010000208         2443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_356422, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000208 DASWNJ010000000
VERSION     DASWNJ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356422"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="VGB85_03855"
     CDS             complement(<1..813)
                     /locus_tag="VGB85_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0133182.1"
                     /translation="MRRAFVTGGSGFVGRNLLEALQARQVPTVALARSDVSAAACAGR
                     GARVARGDLDDVEAMAAAMTGCDVVFHCAAHVLEWDRPEVFARVNVDGTANVIAAAKR
                     ARVERLVHVSTEAVLVGAASHEPIVAANEGRPIPATPLPLYPASKAAAELLVRKAAAD
                     LHTVIVRPRFIWGAGDTSLLPQLIEAVLARRFRWIAGGHYKTSTCHVRNCVEGMLRAA
                     ERGGRGETYFLTDGPPVELRGFLRDLLSTQGFDAGPRTIPRGVARALARAAEA"
     gene            complement(847..1620)
                     /locus_tag="VGB85_03860"
     CDS             complement(847..1620)
                     /locus_tag="VGB85_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133183.1"
                     /translation="MDFTGFMAAFALAAFWLNTVLIAAAGWSECVALRRRYAFLSSGT
                     ARGEVVAGAGPDGAIAAWRVVQVGRSNGRGPILFHDRGRASPVFGGVVQVAGQELGVP
                     AGATAEVWLGPRARARAVACPDAQSFAAAMPAASRAAGWERTVEVPLRAGDAIWFTGQ
                     APENQGNSRYILADFDPRRWRARITAMTAGLVVGLLVVAGACTAACLWPPAFGMVSKL
                     GAFAALVAFNLFQLFGKLHHDAIQPPAAAILGGSWRRPR"
     gene            complement(1622..>2443)
                     /locus_tag="VGB85_03865"
     CDS             complement(1622..>2443)
                     /locus_tag="VGB85_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY0133184.1"
                     /translation="LNAVVIHNHLHRGVFHSHRLNMIWRSVLSFGALYPASANVPSHN
                     LVHHHFGDDGQPDWADPQHVRFGWNLLNLIHFPNIAGPNTFAGVARWAATTKQASFGR
                     QYMTESVVAFGLTGAFLLYDFWPALCFLVLPQFYGARCILRINLIQHDRTDTGSEWNH
                     SRNFVGRAFNWIMCNNGYHTIHHNRAGLHWSELAEAHRREVAPRIDPSLDEPSMVLYL
                     LRRYALSFGRPPQRDVAAAERVQPKAKLAPREARRAEAEQAAALEQATALERATV"
BASE COUNT          387 a          917 c          830 g          309 t
ORIGIN      
        1 cgcctcggcc gcgcgggcga gcgcacgcgc cacgccgcgg gggatcgttc ggggcccggc
       61 gtcgaagccc tgggtcgaga gcaggtcgcg caggaacccg cgcagctcga ccggcgggcc
      121 gtcggtcaaa aaataggtct cgccgcgacc gccacgctcg gccgcccgca gcatgccctc
      181 gacgcagttg cgcacgtggc aggtcgaggt cttgtaatgg ccgccggcga tccagcgaaa
      241 tcgccgggcg agcaccgcct cgatcaattg tggcagcaac gaagtgtcgc ccgcgcccca
      301 gatgaagcgc gggcgcacga tcaccgtgtg caggtccgcc gccgccttgc gcaccaggag
      361 ctccgccgcc gccttgctcg cgggatacag cggcaagggc gtcgcgggga tcgggcgccc
      421 ctcgttggcc gcgacgatcg gctcgtgcga cgcggcgccg acgagcaccg cctcggtgct
      481 gacgtgtacg agccgctcga cccgcgcccg cttcgccgcc gcgatcacgt ttgccgtgcc
      541 gtcgacgttg acccgcgcaa acacctccgg ccggtcccac tcgagcacgt gcgccgcgca
      601 atgaaacacg acatcacagc cggtcatcgc cgccgccatc gcctcgacgt cgtcgaggtc
      661 accgcgggcc acccgcgccc cgcggccggc acaggccgcc gccgacacgt cgctgcgcgc
      721 cagcgcgacc gtcgggacct ggcgcgcctg caaggcctcg agcaagtttc gaccgacgaa
      781 gcccgaacca ccggtgacga acgcgcgacg catggcccga gaccctacca gcctgccccg
      841 cgcgcgtcag cgaggccgac gccacgatcc gccgagaatc gccgccgcgg gcggctggat
      901 cgcgtcatgg tgcagcttgc cgaacagctg gaacaagttg aaggcgacca gcgcagcgaa
      961 tgcgccgagc ttgctgacca tgccgaacgc cggcggccac aaacaggccg ccgtgcacgc
     1021 ccccgcgacc accagcagcc cgaccaccag ccccgcagtc atcgcggtga tccgggcccg
     1081 ccagcgccgg ggatcgaagt ccgcgaggat atatcgggaa tttccctgat tttcaggggc
     1141 ctgtcccgtg aaccagatcg cgtccccggc ccgcagcggc acctcgaccg tgcgctccca
     1201 cccggccgcg cgcgaggccg ctggcatcgc cgccgcgaag ctctgcgcgt cggggcaggc
     1261 gaccgcccgc gcgcgggccc gggggccgag ccacacctcg gcggtcgccc cggccggcac
     1321 gcccagctcc tgaccggcga cctgcacgac cccgccgaac accggcgaag cccggccgcg
     1381 atcgtgaaac aggatcggcc cgcgcccgtt ggatctcccg acctggacca cgcgccacgc
     1441 agcgatcgcg ccgtcgggcc ccgcgccggc gacgacctcg cccctggctg tccccgagga
     1501 caggaacgca taacgccgcc gcagcgccac gcactcgctc cacccggccg cggcgatcag
     1561 cacggtgttg agccagaacg ccgccagcgc gaaggcggcc atgaagccgg tgaagtccat
     1621 ctcagaccgt cgctcgttcg agcgccgtag cctgctcgag cgccgcggcc tgctcggcct
     1681 cggcgcgcct cgcctcccgc ggcgccagct tcgccttcgg ctgcacgcgc tcggccgcgg
     1741 cgacgtcgcg ctgcggcggc cgcccgaagc tcagcgcgta gcgccgcagc agatacagga
     1801 ccatgctcgg ctcgtcgagg ctcgggtcga tgcgcggcgc gacctcgcgg cggtgcgcct
     1861 cggcgagctc cgaccagtgc agcccggccc ggttgtggtg gatcgtgtgg tagccgttgt
     1921 tgcacatgat ccagttgaac gcgcgaccga cgaagttgcg cgagtggttc cactcgctcc
     1981 cagtatcggt gcggtcgtgc tggatcaggt taatgcgcag gatgcagcgc gccccgtaga
     2041 actgcggcaa caccaggaag cacagcgccg gccagaagtc gtacaacaaa aatgcgccgg
     2101 tgagcccgaa ggcgacgacc gactcggtca tgtactgccg gccaaagctc gcctgcttgg
     2161 tcgtcgcggc ccaccgcgcg accccggcga aggtgttcgg cccggcgatg ttcgggaagt
     2221 ggatcagatt gagcaggttc cagccaaatc ggacgtgctg cgggtcggcc cagtcgggct
     2281 ggccgtcgtc gccgaagtgg tgatgcacga ggttgtgcga cggcacgttc gccgacgccg
     2341 gatacagcgc gccgaagctg agcacgctgc gccagatcat gttgagccgg tggctgtgaa
     2401 acacgccgcg gtgcagatgg ttgtggatca ccaccgcgtt gag
//
LOCUS       DASWNJ010000209         5850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_4168, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000209 DASWNJ010000000
VERSION     DASWNJ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4168"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VGB85_03870"
     CDS             complement(<1..711)
                     /locus_tag="VGB85_03870"
                     /inference="COORDINATES: protein motif:HMM:NF024930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133185.1"
                     /translation="MPRTAPPLMRSGIAWFATLSLAAFTSTAGCQGPSGSTASRTIAE
                     LGVGYYHTCLRLEDGTVECWGQCGHHCGMRPEGSTERLPVIGLAHAVELAVGDSHACA
                     RLEDGHVACWGSNYSSGLGQPTPETSSMPLVVPDLADVAEIDIGGAMTCARRADATVT
                     CWGRAAGDFEDSAEAIGRRMPAPIVGITDALALAVDATGGCAQVGAPHRWKCWRWSSG
                     SFGFASGMSRATRNPGLDG"
     gene            complement(912..1613)
                     /locus_tag="VGB85_03875"
     CDS             complement(912..1613)
                     /locus_tag="VGB85_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133186.1"
                     /translation="MRAPKQSLSLILVVLAACPGSSGGETGDDSGTDSDAGTTTKPTS
                     GADSTGSTTDPTTGTITDPTTGTTAEQTTVSTSDTTDDNDTTGAESLCDAAPSFPDPG
                     SSEYLQWVVDGEAYLSLPPPGGAYFFNSYLSVALYSSDFTGSLSFGSINSFPDGISAG
                     TYPCSGTDGMFVNGGALGLANGQAPQSSCVIRLTEDLTDGGRIVGSLSARLVDISGNP
                     TTCVDGRFELTDAAP"
     gene            complement(2162..3028)
                     /locus_tag="VGB85_03880"
     CDS             complement(2162..3028)
                     /locus_tag="VGB85_03880"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0133187.1"
                     /translation="MRALLLVLSLCACGPRSAAPPVPPRSPISLTYLGVAGWQLDAGP
                     TTILVDPYYSRPKDLDGPLASDPAAIAARAPARADLIVIGHSHVDHLLDAPAVALATG
                     AQLLGSLSTARVARAHGVPADRIITVKGGEDFAMTGYSLRVLPSLHSALSDKHEFGGE
                     VAAELAPPLRFDDYAEGGTFAYLVRVAGHEVLFLGTANFIERELEGLRPDVAVIATGL
                     RREIHDYTCRLLRALGQPALVYANHFDDWRGPPVDAPPDDDLRAFVAEVRACSPNTRV
                     VVPRHFVRMDVP"
     gene            3402..4691
                     /locus_tag="VGB85_03885"
     CDS             3402..4691
                     /locus_tag="VGB85_03885"
                     /inference="COORDINATES: protein motif:HMM:NF024209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEY0133188.1"
                     /translation="MRTRLLVAGSWVASIAGCGPTAGEVADASSGTSSSGAQTGDATG
                     GGETTAPTGGETTAPTGGETTVDPMTTTDGTSTTTGSGDDDATGDATAPAPPACDVVE
                     IPDPALRAAIRAALEIPEGPISGEAMATLTELAASGDEWYDLEGLECAVGLEWLSLAG
                     AEYLPFSSELDTISALPKLRRLSLAFTSVSDLTALAGLTTLETLDLEHTKVTDLAPLA
                     GLTQLKTLEFPYTSVADLTPLAGLTALEKVVASATKVADLTPLVGLTQLRELRLDDTD
                     IADLGPLAKLTQLEQLHVGGTAVTDLAPLAGLLALAGLGIENNAITDLKPLTGLPITF
                     LYAPGCKISDITPVASFPTPGFMSLAFNLIEDLDPLLAADWYVEDDGCGLLDLSNNPL
                     SVAALEEVIPTVCTETRLPILAEGAVCMGVLDCPGTY"
     gene            4738..5010
                     /locus_tag="VGB85_03890"
     CDS             4738..5010
                     /locus_tag="VGB85_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133189.1"
                     /translation="MNRRRILGGVLVVAGVGAFVLIWRTIAWMVYGGVRDYVQVAGLL
                     ISAGVAIGGASMSIRHRRVGAQVPAAPTPRARSAGERLRAGEISRK"
     gene            5062..>5850
                     /locus_tag="VGB85_03895"
     CDS             5062..>5850
                     /locus_tag="VGB85_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133190.1"
                     /translation="MTLISNLHSARFTSRLAAVLVALGATACTVDKNLATVSDGDTEV
                     SSGTTDEPTSGTTSDDTTATATATATATSSSTSDGGTTATTSTTSTTDDSTTDDSTTA
                     EPAASCPDHPTVDDCCCFEKSDIYVVNVCTAELEVLCEDAKLLCANGGGMQVDGECES
                     AVDEALVDCALSALMGGKAGSIHINLGSADSPGMWDRDIDYHITGDGGVYRVDDTFLD
                     LSGEYKDTGLYDLKPPEFFTACLAGSIAEKADCLREAVTGAAKDL"
BASE COUNT          895 a         1998 c         2109 g          848 t
ORIGIN      
        1 accgtcgagc cccgggttgc gagtggcccg actcatgcct gaagcgaagc cgaaggaccc
       61 cgagctccag cgccagcact tccatcgatg cggcgctccg acctgcgcgc aacctcctgt
      121 ggcgtcgacc gcgagcgcga gcgcgtcggt gatgccgacg atgggggcgg gcatccgacg
      181 cccgatggcc tcggcgctgt cctcgaagtc ccccgcggcc cgaccccagc aggtgacggt
      241 cgcgtcggct cgacgggcgc acgtcatggc gccgccgatg tcgatctcgg cgacgtcggc
      301 caggtcgggc acgacgagcg gcatcgacga ggtctccggc gtcggctgcc cgagcccgct
      361 gctgtagttg ctgccccagc aggcgacgtg tccgtcctcc agccgggcgc acgcgtggct
      421 gtcgccgacc gcgagctcga ccgcgtgcgc gaggccgatg accggcaggc gttcagtgct
      481 cccctccggt cgcatcccgc aatgatgacc gcactgcccc cagcactcga cggtgccgtc
      541 ctcgagccgc agacacgtgt ggtaatagcc gacgccgagc tcggcgatcg tgcgcgaggc
      601 cgtcgagccg ctcggaccct ggcaaccagc ggtcgaggta aacgcggcga gcgagagcgt
      661 ggcgaaccag gcgatgcccg agcgcatgag cggtggtgcg gtgcgaggca tcgccgcgat
      721 cgtagccggg aacgaccgcc ttcatggcga ctgtcttcgc gcacccggca tctgcggatc
      781 ccgagctttt ccgtgccagg agaacgccgc ggagcacgag gcctgcggcc ggtccgcccg
      841 gctacggtca aggcctttgg agcgacgagg tccgcggatc gtcccctgac cggcctcctt
      901 cgccgacgcg ctcagggagc ggcgtcggtc agctcgaagc gcccgtcgac gcaggtggtg
      961 gggttgccgg agatgtcgac gaggcgggcc gagagcgagc cgacgatgcg cccgccgtcg
     1021 gtcaggtcct cggtgagacg gatgacgcag ctggattgcg gggcctggcc gttcgccagg
     1081 ccgagcgcgc cgccattgac gaacattccg tcggtaccgc tacacggata ggtgcccgcc
     1141 gagatgccgt cggggaacga gttgatcgat ccgaagctga ggctgccggt gaagtcggac
     1201 gagtagagcg cgacgctgag gtagctgttg aagaagtatg cgccgcccgg cggcggcaaa
     1261 gacagatacg cctcgccgtc gaccacccac tggaggtact cgctgctgcc ggggtcgggg
     1321 aagctgggag ccgcgtcgca cagcgattcg gcgccggtgg tgtcattgtc gtcggtcgtg
     1381 tcgctggtcg agacagtggt ctgctcggcg gtggtgccgg tcgtcggatc ggtgatggtg
     1441 ccggtcgtcg gatcggtggt ggagccggtg ctgtcggctc cggaggtcgg cttggtggtg
     1501 gtcccggcgt cgctgtccgt accggagtcg tccccggtct ctccgcccga agagccgggg
     1561 caggcggcga ggacgacgag gatcagcgag agggactgct ttggcgcgcg catcgggcaa
     1621 gagtctacag gccgggctgc gccagataaa cgacgcccgt cgggcgtgct cgcgcagccc
     1681 gtcgggcgtg ctcgcgcggc ccgtcgttcg ccgagcccgc gtggcgtctc cgcctcagga
     1741 gcacacgccg ggtgttacgc tcgccgcatg aaccggcgac ggatcccggg tggcgtgacg
     1801 accggggagc caccatggac ccgacgtcct ccacgggcgc gcccgcgcag accgaggatc
     1861 cgtcgacatc gacaggctcg accagcacga cgggtacgac cgatacgagc acgacgagcg
     1921 agacttcgac gaccaccgat gcgcccgcac ccgtctgcac ggactcgtgc ctctcgaccc
     1981 tgcatgacgg cggtgtgcgg cgaccacaag gtgaaggtcg gcaattttca cggctacgac
     2041 gtcgccaccg acacctggga ggtccgccga cgccgccgga ctatacctcg atcacgccag
     2101 gaggagatac tgccgtccag cggatcgccg cgctcgggcg gtgtccggcg gtcctgggag
     2161 ctcacggcac gtccatgcgc acgaagtgcc gcgggaccac cacgcgcgtg ttcggcgagc
     2221 acgcccggac ctccgcgacg aatgcccgta gatcgtcgtc cggaggggcg tcgaccggcg
     2281 gtccacgcca gtcgtcgaag tggttggcat agaccagcgc cggctggccg agcgcacgca
     2341 gcagtcggca ggtgtagtcg tggatctcgc ggcgcaggcc ggtggcgatc accgcgacgt
     2401 cggggcgcag gccttcgagc tcgcgctcga tgaagttggc ggtcccgagg aacagcacct
     2461 cgtggcccgc gacgcgcacg aggtacgcga aggtgccgcc ctcggcgtaa tcgtcgaagc
     2521 gcaacggcgg ggcgagctcg gccgcgacct cgccgccgaa ttcgtgcttg tcggagaggg
     2581 cggagtgcag gctcggcagg acgcgcagcg agtagccggt catcgcgaag tcctcaccgc
     2641 ccttgacggt gatgatgcga tcggccggga cgccatgggc cctggcgacc cgcgccgtgc
     2701 tgaggctgcc gagcagctgc gccccggtgg cgagcgcgac ggccggcgcg tcgagcaggt
     2761 gatcgacgtg tgaatgcccg atcacgatca ggtcggcgcg ggccggcgca cgggcggcga
     2821 tcgccgcggg atcggacgcg agcggaccgt cgaggtcctt cggccgcgaa tagtacgggt
     2881 cgacgaggat cgtggtcggt ccggcgtcga gctgccagcc cgcgacgccg agataggtca
     2941 gcgagatcgg agatcgcgga ggcaccggcg gcgcggccga gcgcgggccg caggcacaca
     3001 gggagaggac gagcaggagc gcgcgcatgc ggcagggtac gccggacccg gcgccgctgt
     3061 tcccaaagtt cgatcgatct ttttcagtga ggtcgagctg cttcgccccg tcgacctccg
     3121 agggaggacc acctcgaggt gtcggggaca aacagggcct ggcacaggtc gccaccgcct
     3181 cttgttgctc cgaggtagag cgccgaatta ccggtttcat catctgctcc gccattgaga
     3241 tcgaagtatc gttcctgatc ttttgcctgt agggggagtt gccgtcgtcg caggggcggc
     3301 gggcgcctgc gcctgcggtg ggttcctctc ctgctctggg tcggtatcca tcggccgccg
     3361 tcgcgattgc gtagattggc gcgcgccggt accgtggccc gatgcgcaca cgcttactcg
     3421 tcgctggttc ctgggtcgcc tcgatcgcgg gttgcgggcc gacggcgggc gaggtcgccg
     3481 acgcgagctc cgggacgagc tcgtccggcg cgcagacggg cgatgcgacc ggcggaggcg
     3541 agacgaccgc gccgaccggc ggcgagacga ccgcgccgac cgggggcgag acgaccgtcg
     3601 acccgatgac gacgaccgac ggcacgtcga ccacgaccgg gtcgggtgac gatgacgcga
     3661 cgggcgacgc gaccgccccg gcgccgcccg cctgcgacgt cgtcgagatc ccggaccccg
     3721 ccctgcgcgc ggcgatccgc gccgcgctgg agatccccga gggcccgatc tccggtgagg
     3781 cgatggcgac cctgaccgag ctcgcggcgt ccggcgacga gtggtacgac ctcgagggcc
     3841 tcgagtgcgc cgtcggcctc gaatggctgt cgctcgccgg cgccgagtac ctgccgttct
     3901 ccagcgagct ggacacgatc tcggcgctgc cgaagctgcg gcggctgtca ctcgccttta
     3961 cgagcgtctc cgacctgacg gcgctcgccg ggctgacgac gctcgagacg ctcgacctcg
     4021 agcacaccaa ggtcacggac ctcgccccgc tcgccggcct gacgcagctg aagacgctcg
     4081 agtttccgta tacgtcggtc gccgatctga cgccgctcgc gggcctgacc gcgctcgaga
     4141 aggtcgtcgc cagcgccacg aaggtcgccg acctgacgcc gctcgtcggg ctgacgcagc
     4201 tgcgcgagct ccggctcgac gacaccgaca tcgccgacct cgggcccctg gcgaagctga
     4261 cgcagctcga gcagctccac gtcggcggca ccgccgtcac cgacctcgcg ccgctcgccg
     4321 gcctgttggc gctggccggc ctcggcatcg agaacaacgc catcacggac ctgaaaccgc
     4381 tgaccggcct gccgatcacg ttcctgtacg cgccgggttg caagatctcc gacatcaccc
     4441 cggtcgccag cttcccgacc cccggcttca tgtccctcgc gttcaacctg atcgaagacc
     4501 tcgacccgct gctcgccgcc gactggtacg tcgaggacga cggctgcggc ctgctcgacc
     4561 tgagcaacaa cccgctgtcg gtcgcggcgc tggaggaggt catcccgacc gtgtgcaccg
     4621 agacgcgcct gccgatcctg gccgagggtg ccgtgtgcat gggcgtgctg gactgccccg
     4681 gcacgtactg accgcccgac gcaccggctc gcgcgcgccg ggtgctacgc tcgccgcatg
     4741 aaccggcgac ggatcctggg tggcgtgctt gtggtcgcgg gtgtcggcgc gttcgtgctg
     4801 atctggcgga cgatcgcgtg gatggtgtac gggggcgttc gtgattatgt gcaggtcgcc
     4861 gggttgctga tcagcgccgg cgtggcgatc ggtggagcat cgatgtcgat ccgtcatcgc
     4921 cgggtaggcg cgcaggtgcc cgcagcgccg acgccgcgag cgaggtccgc gggcgagcgg
     4981 cttcgcgccg gcgaaatttc tcgaaaataa gtccggggcc caaacagaca cgccggggtc
     5041 tggagaagta gggcaatgac cttgaccttg atctcgaatc ttcattccgc gcgtttcacc
     5101 tcccggctcg cggccgtgct ggtcgccctg ggggcgaccg cctgcaccgt cgacaagaac
     5161 ctcgccacgg tgagcgacgg cgacacggag gtcagctccg ggaccaccga cgagccgacg
     5221 agcggcacga cgagcgacga cacgaccgcg accgcgaccg cgaccgcgac cgcgacctct
     5281 tccagcacga gcgacggcgg caccaccgcc acgacgagca cgacgagcac gaccgacgac
     5341 tccacgaccg acgactccac gaccgcggag ccggcggcca gctgccccga tcatccgacg
     5401 gtcgacgatt gctgctgctt cgagaagtcg gacatttatg tggtgaacgt ctgcaccgcc
     5461 gagctcgagg tgctgtgcga ggacgcgaag ctgctgtgcg cgaacggggg gggtatgcag
     5521 gtcgacggtg agtgcgagag cgcggtcgat gaggcgctcg tcgactgcgc gctctcggcg
     5581 ttgatgggcg gcaaggcggg gtcgatccac atcaacctcg gctcggcaga cagccccggc
     5641 atgtgggacc gcgatatcga ctaccacatc acgggcgacg gcggcgtgta tcgcgtcgac
     5701 gacacgttcc tcgacctcag cggcgagtac aaggataccg gcctctacga cctgaagccg
     5761 ccggagttct tcaccgcgtg cctcgccggg tcgatcgcgg agaaggccga ctgcctgcgc
     5821 gaggccgtga cgggggctgc gaaggatctc
//
LOCUS       DASWNJ010000210         1871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_145058, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000210 DASWNJ010000000
VERSION     DASWNJ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145058"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..1429
                     /locus_tag="VGB85_03900"
     CDS             293..1429
                     /locus_tag="VGB85_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133191.1"
                     /translation="MADLGKQSEFSPEELDRLEDALERWTSADLDPLSKDLSTGLSSG
                     PEDSSLAPHLRARLEDYRSLLAMTRDELPMEDVPDGLLASVLAEAHASAQPRTRPVTR
                     RELAPAGPGLWERLRRSMLLPGFALAGSAALLLWVVQPSSDATLGDDEVLLERTAQTP
                     AQLSRPLDASPAPAAAEAPRLEQNRLAAPELDAAADDKADAQAPAEAAGGSPATAPAD
                     APGSPDSLDPLAEAELKDLAKSDRVPTKKTSKKVLAEPIETYPGLDDVPMAEGVDKEA
                     LRDTIDKADGARRKGGCTEAMSLYLEAMHMSGANSERARARAGYGLCLSSQGRDDTAD
                     KYFDAARALSPAIDAWIKRERGDGPSRKAPAKPSAKAKMEAPLD"
BASE COUNT          261 a          705 c          672 g          233 t
ORIGIN      
        1 gggtgcctcc ggtgcaaacg gccgagggcg ctcgcagcgg cggggccgtg tgattcggca
       61 cgggacatcc cgggccgagg ctcaggaagg tgacttcggg cagaccgcgg accggcgagg
      121 cccgttcatg ccgcccgtgc actccgtcac ccacccgccc tgccactggg gatcgggcct
      181 caccgtccgc gggcggcggc ttgcctgccg cgcgcgagcg ggtgcatgct cgggtagacc
      241 gggagcatcg ggtttggggt cacgagcacg gagcgacgcg agctgaggag ccatggcaga
      301 cctgggcaag caatccgagt tctcccccga ggagctcgac cgcctcgagg acgccctgga
      361 gcgctggacc tcggcggacc tcgatcctct ctcgaaggac ctctcgactg gcctctcatc
      421 tggccccgag gacagctcgc tggcgccgca cctgcgcgcc cgcctcgagg actaccgctc
      481 gctgctggcg atgacccgcg acgagctgcc gatggaggac gtgcccgacg ggctgctcgc
      541 cagcgtgctc gccgaggccc acgccagcgc ccagccgcgc acccgaccgg tcacccgccg
      601 cgagctcgcg cccgccggcc ccggcctatg ggagcgcctg cgccgctcga tgctgctgcc
      661 cggcttcgcg ctggccggct cggccgcgct gctgctgtgg gtcgtgcaac cttcgagtga
      721 cgcgacgctc ggcgacgacg aggtcctgct cgagcgcacg gcccagacgc ccgcgcagct
      781 gtcacgtccg ctcgacgcca gccccgcgcc cgcggccgcc gaggccccga ggctcgagca
      841 gaaccgcctc gccgcgcccg agctcgacgc cgccgcggac gacaaggccg acgcgcaggc
      901 gccggccgag gccgcaggcg ggagcccggc gaccgccccc gccgatgcgc cgggctcgcc
      961 cgactcgctc gacccgctcg cagaggccga gctcaaggac ctggcgaagt ccgaccgcgt
     1021 cccgacgaag aagacgtcga agaaggtgct cgccgaaccg atcgagacct acccaggcct
     1081 cgacgacgtg ccgatggccg agggcgtcga caaggaggcg ctgcgcgaca cgatcgacaa
     1141 ggccgacggg gcgcgccgca agggcggctg caccgaggcg atgagcctct acctcgaggc
     1201 gatgcacatg agcggggcga acagcgagcg cgcccgggcc cgcgccggct atggcctgtg
     1261 cctctcgtcg cagggccggg acgacacggc cgacaagtac ttcgacgccg cccgcgccct
     1321 ctcccccgcc atcgacgctt ggatcaagcg cgagcgcggc gacggcccgt cccgcaaggc
     1381 gcccgccaag cccagcgcca aggcgaagat ggaagcgccg ctcgactgat ctctcgctgc
     1441 cttgttcggg ggctcgagcg gctcgagtat ggcgtgaatg ggtgatgcgg cggtcgggcg
     1501 tggcgtgaat gagggacgcg gggcgaggct tcctgggccg gggaggggcc ggcgggctgc
     1561 gggtccctgc gacgcgggtc ctcccagggg acatgctcgc cgccccatct gtcccgaaag
     1621 acagggtcct gtcaccgacc ctacctcgtg ccgacggaag ttgacggggc gtctgcgcgt
     1681 gacccctggg tcctcccgct gcttcccccg ccagccggcc cctgctcccc gggcggacat
     1741 tgtctgcgcc ccgcttgtcg ccggacgcag agcgttgcgc gagcgatcgc caatcccgat
     1801 gtgcgcgtgc ccgggttgcc gggcgagctt ctcgcagtcg cagtcgcagt cgcagtcgca
     1861 gtcgcagtcg c
//
LOCUS       DASWNJ010000211         1813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_391083, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000211 DASWNJ010000000
VERSION     DASWNJ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391083"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VGB85_03905"
     CDS             <1..738
                     /locus_tag="VGB85_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133192.1"
                     /translation="ADGQPHKLKVHKKSGQVVEVTITATPQELPPLKDIPPFWSVGAA
                     DLDKAPVWARRTLFGHVSPMTMLVAADGGTTDGRQMFGKKRLIMFWDYATREEQAQAA
                     NMMQVLQLAQDDLKKSGVDIMFTQIRFPGNDNGDGTPRQAPMNDTALRKFQADYGVKG
                     KPTLPMFRFPNATEYNAAREVGLEGSTTYIQYLRASPALILLDEGGVVRWHSEGITPA
                     PAGDTTYAGKDDQYTIAQAIDFAMKNL"
     gene            complement(824..>1813)
                     /locus_tag="VGB85_03910"
     CDS             complement(824..>1813)
                     /locus_tag="VGB85_03910"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEY0133193.1"
                     /translation="EQLVDGVLRELEAQAAAPMIEEDYDGPVLFQGPAAAQLLASTVA
                     TQVGGNPAPLGDAGRVRDFEPHWQDALGKPVMPNFLDLEDDPREGFGRYAIDGQGFVA
                     EPLTLVKGGVLRELLMTRTPNKKRAASNGRARVTPSLRHGPTISNLSVKSKKRGMAKA
                     QLERELLRRAREDGYEFAYVVESLRDNNILGPVDREGATGYGGGRKVSLPLPARIVRI
                     DASGKRTLVRGAMFSPVSMRVLRRIRAVGNTSTPVPMRFPPGYNGGFAAETGMDDILS
                     ETVDVQVSTPDLLLDGLEVLVERSENERLPVLAHPLRPTSAPRKGAADEDDDE"
BASE COUNT          268 a          698 c          585 g          262 t
ORIGIN      
        1 gccgacggcc agccgcacaa gctcaaggtc cacaagaagt ccggccaggt cgtcgaggtc
       61 acgatcaccg cgaccccgca ggagctgccg ccgctcaagg acatcccgcc gttttggtcc
      121 gtcggcgccg ccgacctcga caaggcgccg gtctgggccc gccgcaccct gttcgggcac
      181 gtcagcccga tgaccatgct cgtcgcggcg gacggcggca cgaccgacgg ccggcagatg
      241 ttcggcaaga agcgcctcat catgttctgg gactacgcga cccgcgagga gcaggcccag
      301 gccgccaaca tgatgcaggt gctgcagctc gcgcaggacg acctcaagaa gtccggcgtc
      361 gacatcatgt tcacgcagat ccgcttcccc ggtaacgaca acggcgacgg caccccccgc
      421 caggcgccga tgaacgacac cgccctgcgc aagttccagg ccgactacgg cgtcaagggc
      481 aagccgaccc tgccgatgtt ccgcttcccc aacgcgaccg agtacaacgc ggcccgcgag
      541 gtcggcctcg agggctcgac gacctacatc cagtacctgc gcgcctcccc ggcgctgatc
      601 ctgctcgacg agggtggcgt cgttcgctgg cactcggagg gcatcacgcc cgcccccgcc
      661 ggtgacacca cctacgccgg caaggacgac cagtacacga tcgcccaggc gatcgacttc
      721 gccatgaaga acctgtgagc cgcgggccct cgcgggcccc gacatcgcct cgacttcgac
      781 ggccgcgccc tcccgggtgc ggccgttcgt cactcgactg acttcactcg tcgtcgtcct
      841 cgtccgccgc gcccttcctg ggcgcggagg tcggtcgcag cgggtgggcg agcactggca
      901 ggcgctcgtt ctcgctgcgc tcgacgagca cctcgagccc gtcgagcagc aggtccggtg
      961 tgctgacctg gacgtcgacg gtctcgctga ggatgtcgtc catcccggtc tcggccgcga
     1021 agccgccgtt gtagccgggt gggaagcgca tcggcaccgg ggtcgaggtg ttaccgaccg
     1081 cgcggatcct ccggagcacg cgcatcgaca ccgggctgaa catcgcccca cgcaccagcg
     1141 tgcgcttgcc cgaggcgtcg atgcgaacga tgcgcgcggg caacggcagg ctgaccttgc
     1201 ggccgccgcc gtagccggtc gcgccctcgc ggtcgaccgg gccgaggatg ttgttgtcgc
     1261 gcagcgactc gaccacgtac gcgaactcgt agccgtcctc gcgggcgcgc cgcagcagct
     1321 cgcgctcgag ctgcgccttc gccatgccgc gcttcttcga cttcacgctc aggttggaga
     1381 tcgtcggtcc gtggcgcagc gacggggtca cccgcgcgcg cccgttgctg gccgcccgct
     1441 tcttgttcgg cgtccgcgtc atcaacagct cgcgcagcac cccgcccttg accagcgtca
     1501 gcggctccgc gacaaagccc tgcccatcga tcgcgtagcg gccgaagccc tcgcgcgggt
     1561 cgtcctcgag gtcgaggaaa ttcggcatca ccggcttgcc gagcgcgtcc tgccagtgcg
     1621 gctcgaagtc ccgcacccgc cccgcgtcgc cgagcggcgc cgggttgcct ccgacctgcg
     1681 tcgccaccgt cgacgccagc agctgcgccg ccgccgggcc ctggaacagc accgggccgt
     1741 cgtagtcctc ctcgatcatc ggcgcggccg cctgcgcctc gagctcgcgc agcaccccgt
     1801 cgacgagctg ctc
//
LOCUS       DASWNJ010000212         6783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_428103, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000212 DASWNJ010000000
VERSION     DASWNJ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6783
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428103"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="VGB85_03915"
     CDS             complement(<1..1314)
                     /locus_tag="VGB85_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133194.1"
                     /translation="MPLSASRSHHHAALLALLTAACGDAGTTLTQGSTGPGEPVTTGG
                     IDPSIATTLTTGTPTTGEPATTGDSEDNTTGEPATYCRQLDISLVANPGLNIYSPGHR
                     AAFAAFFADMVETTGARVRLLANSGTELTLKTDCLLPLGNAPDDPVLVYGEDGVVDPD
                     APAALECLLTALDKYVSDFDEGNFYFSGLLFPVLELEQWPAPGATGLALMLAFSDDAQ
                     DNLYAQPGIAAEAYLRLVGEGDRRRVAALTYGRDAGKLEIFGLTLSEHSRHHERDETD
                     LLTALAQWTPIARQVCEDYDYEPPFQEDPPSGCKRIDVLFNVDGSGSMTEEQDALRGT
                     NGMAPVFAEFTDALLAELTDVEDFHVGVVSPQEGVTLLGTHRNFPETPESPETACGVP
                     PGQRWLVGPSPTLAEQFACIASTRSGTDEVTAYNTAEALHDPANAG"
     gene            1383..2405
                     /gene="add"
                     /locus_tag="VGB85_03920"
     CDS             1383..2405
                     /gene="add"
                     /locus_tag="VGB85_03920"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEY0133195.1"
                     /translation="MTGLDPRLDDLILRLPKVELHLHLEGSIHPELALRLAHRRGRTL
                     PGMDSGADGLREHYRFASFKDFLRMYIAISACLAEAEDFCEITVALARDLAAQRVAYA
                     EVTFTPMTHVARGVDPAVMLAGLAEGRARAKAEYGVELAWVFDIVRCFRDQSEPTLEL
                     ALRGRDDGVIGLGFAGPEAPEWPTAPFAETFMRAKAEGLHSLPHAGEMAGPASIWEAI
                     DLLGAERIGHGVRAVDDPALLEHLVRTQIPLEVCPTSNLGIGLYADLAAHPLPQLLRA
                     GVAVSLASDDPPMFATSLVEEYRRCAAAYGWDAAQLRALAAAAVHHSFLAPERKAELL
                     RQQDAV"
     gene            2456..5488
                     /locus_tag="VGB85_03925"
     CDS             2456..5488
                     /locus_tag="VGB85_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133196.1"
                     /translation="MQRHRRWTRPLILVAALLACKTQSGADASADAASDPAKPGAVAA
                     VDTKLEPLPALRTGTRTALAGKELAGKVTGRSTDATHEYLELALPLSGDDTSLDFDWL
                     HVELTGSGPGDIALVASRMGDSEARVVVRRKPGTADAEVTGALFGERWNDGKRQWTRV
                     PFRADSKAAKEQAELKPRFVDAFAAMADADWRAAHPWLQFAAGRVHALIPNGLRPGGP
                     QGTDLVRPTVRTDLSQLMDTTTGVMSMQEALQHDRGLRVDRSDDTRTISVGELGLPPL
                     DAHPFPAMQAKLPDPAAGAPEPLAAAVPADFWYARVDDIRLMLRLMDEADAWITPVVQ
                     ILQRNPEDRHLADRYQTQLGLRRTDMAKLFGHTVVGQVALTGSDPYLREGSDVTMIFS
                     VKQQAIFDQELTRHIEALRAEVPGLVSATRDYNGVTIAESRDPTGAVRQQRAQVGELA
                     IVSNSPRACERVIDAVQGKTARLGDEPDLKYMLARDPGTHQAFVFLSDKFIAAVIGPQ
                     QKVLAARRQLALAELLTPGYAALLHGWLFGAAPASTDALVASGLLFADELKHRDGAAI
                     AFAPGSAASSPWGRPSALTPLIDLPPVTQVSAAEKTAYENFVSGYQQYWKQFIDPVAI
                     RIDVEDTGATSVAQIDVRILPLISATDYSEIEEIVGGTRVQVSAVDRGLQGVWAVGKD
                     ARLRKDLDGVMHAATGQTDIGIGWLGDWVLLGIEDRAALVELMARFDFPVQLAPASAE
                     RDAFNDIEMWRLVGRFPVYAAAEVKNPAMLVATLAAIKTMLGGVAPGMVDWGETAKHR
                     DLPIVRVGASKSAPLPNREIADAVALYYVQTGEAVVLAIDLETLKRAVDRVLDGKAPK
                     ASAEGSSQFVVEGHSQAGAPLWTALLWLIQGQANEAQSGARRSAEILMRGDPGVQGDP
                     KALAQRGLDYFGFAPVTAQGDSAFTLAPEGAGDALLGTSITPIFVPLPIAGSPVERLM
                     QRLTGVRGEVSFDKEPDPAGPNARSLHTRFSLHLGAAQ"
     gene            complement(5569..5991)
                     /locus_tag="VGB85_03930"
     CDS             complement(5569..5991)
                     /locus_tag="VGB85_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133197.1"
                     /translation="MLSTITLLASTLLVAPNFTAPPTAPQGGLLDGIGIGDGGGLLDL
                     DLDLDACLDLDLDVDLDCEIEVGAECHADCEVNVDAYCDAQANANKWNAKAHAQCEAE
                     VEAQCHANCDIDGSVFCEGDIYVGADVCLDVSLDLDIL"
BASE COUNT          981 a         2272 c         2535 g          995 t
ORIGIN      
        1 gccggcgttg gccgggtcgt gcagcgcctc ggcggtgttg taggcggtga cctcgtcggt
       61 gcccgagcgg gtcgaggcga tgcaggcgaa ttgctcggcg agcgtcggcg aggggccgac
      121 cagccagcgc tgcccgggtg gcacgccgca ggcggtctcc gggctctcgg gggtctcggg
      181 gaagttgcga tgggtgccga gcagggtgac gccctcctgc ggcgagacga cgccgacgtg
      241 gaagtcctcg acgtcggtga gctcggcgag caatgcatcg gtgaactcgg cgaacacggg
      301 ggccatgccg ttggtgccgc gcagggcgtc ctgctcctcg gtcatgctgc cggagccgtc
      361 gacgttgaac agcacgtcga tgcgcttgca gcccgagggc gggtcctcct ggaacggcgg
      421 ctcgtagtca taatcctcgc agacctggcg ggcgatcggt gtccactgcg cgagcgcggt
      481 cagcaggtcg gtctcgtcgc gctcgtggtg gcggctgtgc tcgctgagcg tcaggccgaa
      541 gatctcgagc ttgccggcgt cgcggccgta ggtgagcgcg gcgacgcggc ggcggtcgcc
      601 ctcgccgacg aggcgcagat acgcctccgc ggcgatgccc ggctgcgcat acaagttgtc
      661 ctgcgcgtcg tcgctgaacg cgagcatcag cgcgaggccg gtcgcgcccg gggccggcca
      721 ctgctcgagc tcgagcaccg ggaacagcag gcccgagaag tagaagttgc cctcgtcgaa
      781 gtcgctgacg tacttgtcga gcgcggtcag caggcactcg agcgcggccg gggcgtcggg
      841 gtcgacgacg ccgtcctcgc cgtagacgag caccgggtca tccggggcgt tgcccagcgg
      901 cagcaggcag tcggtcttga gcgtcagctc ggtgccgctg ttagcgagca ggcgcacgcg
      961 cgcgccggtg gtctcgacca tgtcggcgaa gaacgccgcg aacgcggcgc ggtgccctgg
     1021 cgagtagatg ttgaggccag ggttggcgac gagcgagatg tcgagctggc ggcaataggt
     1081 cgcgggctcg ccggtggtgt tgtcctcgct gtcgccggtg gtcgcgggct cgccggtggt
     1141 cggggtgccg gtggtgagcg tcgtggcgat cgacgggtcg atgccgccgg tcgtcaccgg
     1201 ctcgccgggg ccggtcgagc cctgggtgag cgtggtgccg gcgtcgccgc acgcggccgt
     1261 caggagggcg agcagggcgg cgtggtggtg cgatcggctc gcggagagcg gcatcgaacg
     1321 atctttacgc gaggtcgagc ggtcagacca gcgggcccgg cgtttcggct atcgttcgtc
     1381 gcgtgaccgg cctcgacccg cgcctcgacg acctgatcct gcgtctaccc aaggtcgagc
     1441 tgcacctgca cctcgagggc agcatccacc ccgagctggc gctgcgcctg gcgcaccgtc
     1501 gcgggcgcac tcttccgggc atggacagcg gcgccgacgg cctgcgcgag cactaccgct
     1561 tcgccagctt caaggacttc ctgcgcatgt acatcgcgat ctcggcgtgc ctggcggagg
     1621 ccgaggattt ctgcgagatc accgtggcgc tcgccaggga cctcgcggcg cagcgggtcg
     1681 cctacgccga ggtcaccttc acgccgatga cccacgtcgc ccgtggggtg gaccccgcgg
     1741 tgatgctcgc cgggctcgcc gagggccgcg cgcgcgcgaa ggcggagtac ggcgtcgagc
     1801 tggcgtgggt cttcgacatc gtgcgctgct ttcgtgatca aagcgagccg accctcgagc
     1861 tggcgctgcg cgggcgtgac gacggcgtga tcgggctcgg cttcgccgga cccgaggcgc
     1921 ccgagtggcc gaccgcgccg ttcgcagaga cgtttatgcg ggccaaggcg gagggcctgc
     1981 actcgctgcc gcacgcgggc gagatggccg gaccggcgag catctgggag gcgatcgatc
     2041 tgctcggcgc ggagcgcatc ggtcacgggg tgcgtgcggt cgacgaccct gcgttgctcg
     2101 agcacctggt ccgcacgcag atcccgctcg aagtctgccc gacgagcaac ctcgggatcg
     2161 ggctgtacgc cgacctcgcg gcgcatccgc tgccgcaact cctgcgggcg ggagtcgccg
     2221 tgtcgctcgc cagcgacgac ccgccgatgt tcgcgacctc gctcgtcgag gagtatcgcc
     2281 gctgcgccgc cgcctatggc tgggacgccg cgcagcttcg cgcgctcgcg gccgctgcgg
     2341 tgcaccacag cttcctcgcg ccggagcgca aggcggagct gctgcggcaa caggacgcgg
     2401 tataggcccg gtggcgagcg cgcagccggg gccgggcgcc tgatactctg cgcgcgtgca
     2461 acgtcatcgt cgctggactc gtcccctcat cctcgtcgcc gccctcctcg cgtgcaagac
     2521 ccagagcggc gccgatgcca gcgccgatgc tgcgagtgac ccggccaagc ccggcgccgt
     2581 ggcggccgtc gataccaagc tcgagccgct gcctgcgctg cgcaccggga cccgcacggc
     2641 gctcgccggc aaggagctcg ccggcaaggt cacggggcgc agcacggacg cgacgcacga
     2701 gtacctcgag ctggccctgc cgctctcggg cgacgatacg agcctcgact tcgactggct
     2761 gcacgtcgag ctgacgggca gcggacccgg cgacatcgct ctggtggctt ctcgcatggg
     2821 cgacagcgag gcgcgcgtgg tcgtgcggcg aaagcccggc accgccgacg ccgaggtcac
     2881 cggcgcgctg ttcggcgagc gctggaacga cggcaagcgg cagtggacgc gggtgccgtt
     2941 tcgcgccgac agcaaggcgg cgaaggagca ggccgagctc aagccgcgct tcgtcgacgc
     3001 cttcgccgcg atggccgacg ccgactggcg cgccgcgcat ccgtggctgc aattcgccgc
     3061 gggccgcgtg cacgcgctga tcccgaacgg cctgcggccg ggcgggccgc aggggacgga
     3121 cctcgtccgg ccgaccgtgc gcaccgacct ctcgcagctg atggacacga ccaccggcgt
     3181 gatgtcgatg caggaggcgc tgcaacacga ccgcggcctg cgcgtcgatc gcagcgacga
     3241 cacgcggacg atctccgtcg gcgagctcgg cttgccgccg ctcgacgccc accccttccc
     3301 ggcgatgcag gccaagctgc ccgacccggc cgccggcgcg cccgagccgc tggccgcggc
     3361 ggtgccggcc gacttctggt acgcgcgcgt cgacgacatc cgcctgatgc tgcgcctgat
     3421 ggacgaggcc gacgcctgga tcacgccggt cgtgcagatc ttgcagcgca accccgagga
     3481 ccggcacctc gccgaccgct accagaccca gctcggcctg cgccgcacgg acatggccaa
     3541 gctgttcggg cacacggtcg tcggccaggt cgcgttgacc ggcagcgacc cttatctgcg
     3601 cgagggcagc gacgtcacga tgatcttcag cgtgaaacag caggcgatct tcgaccagga
     3661 gctcacccgg cacatcgagg cgctgcgcgc cgaggtcccg gggctggtga gcgcgacccg
     3721 tgattacaac ggcgtcacga tcgccgagag ccgcgacccg acgggcgcgg tccggcagca
     3781 gcgcgcgcag gtcggcgagc tggcgatcgt gtccaactca ccgcgcgcct gcgagcgggt
     3841 gatcgacgcg gtccagggca agacggcccg gctcggcgac gaacctgacc tcaagtacat
     3901 gctggcgcgc gaccccggga cccaccaggc gttcgtgttc ctgagcgaca agttcatcgc
     3961 cgcggtgatc ggcccgcagc agaaggtgct cgcggcgcgc cgccagctgg ccctcgccga
     4021 gctgctgacg cccggctacg cggccctgct gcacggctgg ctgttcggcg cggcgccggc
     4081 gagcacggac gcgctggtcg ccagcggcct gctgttcgcc gacgagctca agcaccgcga
     4141 cggcgcagcg atcgcgttcg cgccgggcag cgcggcgagc tcgccgtggg gtcgcccctc
     4201 ggcgctgaca ccgttgatcg acctcccgcc ggtgacgcag gtcagcgcgg ccgagaagac
     4261 cgcctacgag aattttgtca gcggttatca gcagtactgg aagcagttca tcgacccggt
     4321 ggcgatccgg atcgacgtcg aggacaccgg cgcgacctcg gtcgcgcaga tcgacgtccg
     4381 gatcttgccg ctgatctcgg ccacggatta cagcgagatc gaggagatcg tcggcggcac
     4441 gcgcgtgcag gtcagcgcgg tcgaccgcgg tctgcagggg gtgtgggcgg tcggcaagga
     4501 cgcccggctg cgcaaggacc tcgacggggt catgcacgcg gccaccggcc agaccgacat
     4561 cggcatcggc tggctcggcg actgggtgct gctcggcatc gaggaccgcg ccgcgctcgt
     4621 cgagctgatg gcccgcttcg acttccccgt gcagctggcg ccggcgtcgg cggagcgcga
     4681 cgcgttcaac gacatcgaga tgtggcgcct ggtcggccgc ttcccggtgt acgccgcggc
     4741 ggaggtcaag aacccggcga tgctggtggc gacgctcgcg gcgatcaaga cgatgctcgg
     4801 cggggtggcg ccgggcatgg tcgactgggg cgagaccgcc aaacaccgcg acctcccgat
     4861 cgtccgcgtc ggcgcgagca agtcggcgcc gctgccgaac cgcgagatcg ccgacgccgt
     4921 cgcgctgtac tacgtgcaga ccggcgaggc cgtggtgctg gcgatcgacc tcgagacgct
     4981 caagcgcgcg gtcgaccgcg tgctcgacgg caaggcgccc aaggccagcg ccgagggcag
     5041 ctcgcagttc gtggtcgagg gccactcgca ggcgggcgcg cccctgtgga ccgcgctgct
     5101 gtggctgatc cagggccagg cgaacgaggc ccagagcggg gcgcggcgct cggcggagat
     5161 cctcatgcgc ggcgaccccg gcgtgcaggg cgacccgaag gcgctcgcgc agcgcgggct
     5221 cgactacttc ggcttcgcgc cggtgacggc ccagggcgac agcgcgttca ccctcgcgcc
     5281 cgagggcgcc ggcgacgcgc tgctcggcac gagcatcacg ccgatcttcg tgccgctgcc
     5341 gatcgcaggc tcgccggtcg agcgcctgat gcagcgcctg accggcgtgc gcggcgaggt
     5401 cagctttgat aaagagcccg accccgccgg gcccaacgcc cgcagcctgc acacccgctt
     5461 ctcgctgcac ctcggcgcgg cgcagtgatc cctttttaag aaacaaaaac gccgacgggc
     5521 gccagcgcag tgcgcgggcg cccgggggcg ggagcggcca gggccggctt acaggatgtc
     5581 gaggtcgagg ctgacatcga ggcaaacgtc ggcgccgacg tagatgtcac cctcgcagaa
     5641 gacggagccg tcgatgtcgc agttggcgtg gcactgggcc tcgacctcgg cctcgcactg
     5701 agcatgggcc ttggcgttcc acttgttggc gttggcctgg gcgtcgcagt aggcgtcgac
     5761 gttgacctca cagtcggcgt ggcactcggc accgacctcg atctcgcagt ccaggtccac
     5821 gtcgaggtcg aggtcgaggc aggcgtcgag gtcgaggtcg aggtcgagca gaccgccacc
     5881 gtcgccgatg ccgatgccat cgagcaggcc accctggggc gcggtggggg gcgcggtgaa
     5941 gttgggggcg acgaggaggg tggaggcgag cagggtgatg gtggagagca tgggagtggt
     6001 cctttgttcc cgaacagcgg tcggggcagt gcgtcttgag ggccgccggg ggattccgtc
     6061 gcggcctcga tgatttgatt atggcgggcg aagttttcgc ggccacgacg acgagcccgg
     6121 cgacgagtga aacagcggcg gcgcggatcg cctatttgcc aacagcggcg cgtgcaaggg
     6181 ggaattgagg cattttggca aatttattcg cggtcgtgcg agcgacggta aaactgtcgc
     6241 aacgcggttt tgggccggcc tcgcaaagct caatgtgtat tgttcatgcc gcacccggac
     6301 gccgcgatag atcgcatacc tcgcatcgcg gcgcggggcg cgggaagggc tgctgaaagg
     6361 taaatatttg atatcatgat gttttcatcc catgcccatg gcgcgccccg cggcgttctg
     6421 tggggccctt taagaagtgt attcggaagg atacatgggg gagatatcca cctgtgtctg
     6481 ggtgttatga tcttgggtag tgggctgaga tatcgaagtt tgcgcggcgg tgggcctcgc
     6541 gggggatctg gcgcccgctc gggcgacgaa acgaccgcgg gcgtccacgc agttgcgtgg
     6601 acgcccgcgg cggtgcgcgc ggctcgtctg ggccgaagct gcaaaacgac cgcgggcgcc
     6661 cacgcattgc gtggacgccc gggtggcgcg gtgcgagccg gaggctcgtg ctcagcgcgg
     6721 cttacaggtc gaggtcgagg ctgacatcga ggcagatgtc ggcgccgacg tagatgtcgc
     6781 cct
//
LOCUS       DASWNJ010000213         1811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_640386, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000213 DASWNJ010000000
VERSION     DASWNJ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640386"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VGB85_03935"
     CDS             <1..316
                     /locus_tag="VGB85_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133198.1"
                     /translation="GANLNEQPPASRCAACGTPGARVPVVAAPGDEDDARPVPGAKGK
                     GPKLKADPALLKDAEGEAPWKAALRTIALGDEEDDDAEEADEADGPADATRPKGPGKL
                     RN"
     gene            599..784
                     /gene="rpmF"
                     /locus_tag="VGB85_03940"
     CDS             599..784
                     /gene="rpmF"
                     /locus_tag="VGB85_03940"
                     /inference="COORDINATES: protein motif:HMM:NF013908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEY0133199.1"
                     /translation="MAVPKKRKSKSARDMRRANHDKRSPPTLVACSNCEEATLSHRVC
                     PACGFYKGRQVVAVAQE"
     gene            830..>1811
                     /gene="plsX"
                     /locus_tag="VGB85_03945"
     CDS             830..>1811
                     /gene="plsX"
                     /locus_tag="VGB85_03945"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEY0133200.1"
                     /translation="MTTSSTAADLPIALDAFGGDNCPDVEVKAAVQAGREGIPVLLVG
                     DRDKLAAALARLGIHGIDPDGAVPGLPLRIHHASEVITMDDTPSKVVRSKTDASMTVC
                     FDLVKQGKARAVVSAGNSGAMLACGLLKFGRLKGIDRPAIVSSIPRIGGQCVLLDMGA
                     NVECKPINLVQFAVLGAIFARTSHGVARARVGVLSNGTEEGKGTELTRAAHRALGAQP
                     SPDFSYVGYVEGKDIFRETCDVVVTDGFTGNVALKVIEATAVAFAAQLRAAVEERTLS
                     KVGALLMRPALALFKSRLDPDTYGGAPLLGVDGVTIISHGGASPTALFNGI"
BASE COUNT          281 a          690 c          580 g          260 t
ORIGIN      
        1 cggcgccaac ctcaacgagc agccgcccgc gtcccgctgc gccgcctgcg gcacccccgg
       61 cgcccgtgtc cccgtggtcg ccgcgcccgg cgacgaggac gacgcccgcc ctgtccccgg
      121 cgccaagggc aagggcccca agctcaaggc cgacccggcc ctcctaaagg atgcggaggg
      181 cgaggccccg tggaaggccg ccctgcggac gatcgccctt ggcgatgaag aagatgatga
      241 tgccgaggaa gccgacgagg ccgacggccc cgccgacgcc acccgcccga agggacccgg
      301 caagctgcga aactgatccg ccgcaggccc gcgcgatccg gctcgatctc tcccgtgatc
      361 ctgctggatc ctgctggctc ctgcctggat ccgcccctcg cgccgtccct tgagatttca
      421 catgaaccgc tgcgaaccgg gccctggccc cgcccaaacc cgcgaagcag cgcccttgac
      481 cccttgagct cgtgtcgcgc gcctgtctct ctctgccgca tggtgcggca aaaaaagcct
      541 caccgcgcga caaaaatcgc tacactcccg ccccttcccg agataacggt acctcgccat
      601 ggctgttccg aagaaaagaa agtccaagtc tgcccgcgac atgcgtcgcg ccaaccacga
      661 caagcgctcg ccgccgacgc tcgtcgcctg ctcgaactgc gaggaggcca cgctcagcca
      721 tcgcgtgtgc ccggcctgcg gcttctacaa gggccgccag gtcgtcgccg tcgcccagga
      781 gtgatcctcc ggcggcgctc gtgtcagggt ggatcccggc ctcgtgtcga tgacaacctc
      841 gtccaccgcg gctgacctgc cgatcgcgct cgacgccttc ggcggggaca attgtcccga
      901 tgttgaggtg aaggcggcgg tccaggccgg ccgtgagggt atcccagtcc tgctcgtcgg
      961 cgaccgggac aagctcgccg ccgcgctggc gcggctcggc atccacggga tcgaccccga
     1021 cggcgccgtc ccgggccttc cgctgcggat ccaccatgcc tcggaggtca tcaccatgga
     1081 cgacaccccg tccaaggtgg tgcgcagcaa gaccgacgcc tcgatgacgg tctgcttcga
     1141 cctcgtcaag cagggcaagg ccagggccgt cgtctccgcc ggtaactccg gggcgatgct
     1201 ggcctgcggc ctgctcaagt tcggtcgcct caagggcatc gaccgcccgg cgatcgtctc
     1261 cagcatcccg cggatcggcg gccagtgcgt gctgctcgac atgggcgcca acgtcgagtg
     1321 caagccgatc aacctcgtgc aattcgccgt gttgggggcg atcttcgccc gcaccagcca
     1381 cggggtcgcc cgcgcccgcg tcggcgtgct gtccaacggc acggaagagg gcaagggcac
     1441 cgagctgacc cgcgcggcgc accgggccct cggcgcccag ccgagcccgg atttttcgta
     1501 cgtcggctac gtcgagggca aggacatctt ccgcgagacc tgcgacgtcg tcgtcaccga
     1561 tggcttcacc ggcaacgtcg cgctcaaggt gatcgaggcg accgcggtcg cgttcgcggc
     1621 ccagctgcgc gcggccgtcg aggagcgcac cctgagcaag gtcggcgccc tgttgatgcg
     1681 cccggcgctg gccctcttca agtcacgcct cgatcccgac acctacgggg gcgccccgct
     1741 gctcggcgtc gacggcgtga cgatcatcag ccacggcggc gcgagcccga cggccctgtt
     1801 caacggcatc c
//
LOCUS       DASWNJ010000214         2808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_215339, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000214 DASWNJ010000000
VERSION     DASWNJ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215339"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="VGB85_03950"
     CDS             complement(<1..1067)
                     /locus_tag="VGB85_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133201.1"
                     /translation="MASAAPGPDEAYHSVVQAVARPRFDAATQSQLGTLGLEVRHVTW
                     EDTARYKGSAVGPNISDMTIQVQSRSRGQAFLTCMPVVRAPNFGDRTGDLPIDLVRLR
                     VGNERGAPLRTIGLAEYLGDLRAHLTRPASWAGEQRSLLAPRDTHVLVSAQACLLPVP
                     RAGLATFTPVIFNYQARRGDPAVLAILATREGTSATVIDNQRDGFKVMGSHGQRLLFN
                     HVGERAPFTAQRAGEYLPPGTGEAGGAEAASAVLLIQVPLRQRPDPRVAHMDFMELER
                     ERGFSDVEPAVIGHGEVEGPFTEIDDLPIVRDERFPIRVTVQFYKATSNGSLDEDTLV
                     DIAAQIERVYADAVAVGSLVDA"
     gene            1202..2599
                     /locus_tag="VGB85_03955"
     CDS             1202..2599
                     /locus_tag="VGB85_03955"
                     /inference="COORDINATES: protein motif:HMM:NF011649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HEY0133202.1"
                     /translation="MVYRGSAGTSSVAYDDLRVLMARASPRRSADELAGLAAGSAEER
                     ALAQLALADVPLRRFIDAPLIDPDTDEVARLIAEEHDAAAFAPVASLTVGELREWLLD
                     DLRTPEDLAALRPGLIPDMAAAVSKLMRNQDLVTAAQRCRVTTRFRTTIGLPGRLATR
                     LQPNHPTDDARGVAVSILDGLLLGAGDAVIGINPASDSVAGTRRLLDLVEALRVRHDI
                     PTQSCVLCHVTTAMDLMRQGAPVDLVFQSIGGTEASNRGFGIDLAMLREARQMARERQ
                     RGTLGDDVMYFETGQGSALSAGAHRGLDAQTCETRAYAVARHFGPLLVNTVVGFIGPE
                     YLYDGRQIVRAGLEDHFCGKLLGLPMGVDVCYTNHADADQNDMDALLTMLGVAGCTFI
                     MGVPGADDVMLGYQSTSFHDALYLRRALGLRPAPEFEAWLERVGVFDPATHSLRGAPP
                     AALLRAMAALPGGDA"
     gene            2596..>2808
                     /locus_tag="VGB85_03960"
     CDS             2596..>2808
                     /locus_tag="VGB85_03960"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF017768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase light chain EutC"
                     /protein_id="HEY0133203.1"
                     /translation="MSSDELRRAVARVTSARVLLGRAGPALPTRAWLEFQLAHARARD
                     AVLAPCDLEGLASALRARAVEAVVVRS"
BASE COUNT          367 a          989 c         1051 g          401 t
ORIGIN      
        1 gcgtcgacga gagatccgac cgcgaccgcg tcggcgtaca cccgctcgat ctgcgcggcg
       61 atatctacaa gtgtgtcctc gtcgagcgat ccgttgctcg tcgctttata aaattgcacg
      121 gttacgcgga tcgggaaccg ctcgtcgcgc acgatcggca ggtcgtcgat ctcggtgaag
      181 ggcccctcga cctcgccgtg cccgatcacc gccggctcga cgtcggagaa gccgcgctcg
      241 cgctcgagct ccatgaagtc catgtgggcc acgcgcgggt caggccgctg gcgcagcggg
      301 acctggatca gcagcaccgc gctcgctgcc tcggccccgc cggcctcacc tgtcccgggg
      361 ggcaggtact cgccggctcg ctgcgcggtg aacggcgccc gctcgccgac gtggttgaac
      421 agcaggcgct ggccgtggga ccccatgacc ttgaagccgt cgcgctggtt gtcgatcacc
      481 gtcgccgagg tgccctcgcg cgtcgccagg atcgccagca ccgccgggtc accgcgtcgc
      541 gcctgataat tgaagatcac cggcgtaaac gtcgcgagcc cggcgcgcgg caccggcagc
      601 aggcaggcct gcgcgctgac cagcacgtgg gtgtcgcgcg gggcgagcag cgagcgctgc
      661 tctcccgccc agctcgccgg gcgcgtcaga tgggcccgca gatcgccgag gtactcggcg
      721 aggccgatcg ttcgcagcgg cgcgccgcgc tcgttgccga cgcgcaggcg cacgaggtcg
      781 atcgggaggt cgccggtgcg atcgccgaag ttcggcgccc gcacgaccgg catgcacgtc
      841 aaaaacgcct gcccgcggct acgactctgg acctggatcg tcatgtcgct gatgttcggc
      901 ccgaccgcgg accccttgta acgggccgtg tcctcccagg tcacgtggcg cacctcgagc
      961 ccgagcgtgc cgagctgcga ctgcgtggcg gcgtcaaagc gcggccgcgc gaccgcctgc
     1021 acgacggagt gatacgcctc gtccggaccc ggtgccgcgc tcgccatcca ccgagcatac
     1081 gccagctgcc gcggcgtgga tccgtgcgcg gacagcagca gcccgtgcgt cggccccggc
     1141 gcgcgctcgc ccggagaatt catgccgggc gtcgcggcgc cggtggtaca ttggcgtcgc
     1201 gatggtgtac cgggggtcgg caggcacgag ctcggtcgcg tacgacgacc ttcgggtgct
     1261 gatggcacgc gcctcgccgc ggcgctcggc ggacgagctg gccgggctcg cggcgggcag
     1321 cgcggaggag cgggcgctgg cgcagctggc cctcgcggac gtgccgctgc gtcgcttcat
     1381 cgacgcgccg ctgatcgacc ccgacaccga cgaggtcgcg cggctgatcg ccgaggagca
     1441 cgatgcggcc gcgttcgcgc cggtcgcgtc gctgacggtc ggggagctgc gcgagtggtt
     1501 gctcgacgac ctgcgcacgc cggaagatct ggcggcgctg cggcccgggc tgatcccgga
     1561 catggcggcg gcggtgtcga agttgatgcg caaccaggac ctggtgaccg cggcgcagcg
     1621 ctgccgggtg acgacgcgct ttcgcacgac gatcgggctg ccggggcggc tggcgacgcg
     1681 cctgcagccg aaccacccca cggacgacgc ccgcggcgtc gcggtcagca tcctcgacgg
     1741 cctgctgctc ggggccgggg acgcggtgat cgggatcaac ccggcgagcg acagcgtggc
     1801 gggcacgcgg cgcctgctgg acctcgtcga ggcgctgcgc gtgcgccacg acatcccgac
     1861 gcagagctgc gtgctgtgcc acgtgacgac cgcgatggat ctgatgcgcc agggggcgcc
     1921 ggtcgacctc gtgttccagt cgatcggcgg gaccgaggcg agcaaccgcg gcttcgggat
     1981 cgacctggcg atgttgcgcg aggcgcggca gatggcgcgc gagcgccagc gcgggaccct
     2041 cggcgacgat gtcatgtact tcgagaccgg gcagggcagc gcgctctcgg cgggcgcgca
     2101 tcggggcctc gacgcgcaga cctgcgagac gcgggcctac gcggtggcgc ggcacttcgg
     2161 gccgctgctg gtcaacacgg tcgtcggctt catcggcccc gaatatctat atgatggtcg
     2221 ccagatcgtc cgcgccgggc tcgaggacca cttctgcggc aagctgctcg gcctgccgat
     2281 gggcgtcgac gtctgctaca cgaaccacgc cgacgccgac cagaacgaca tggacgcgct
     2341 gctgacgatg ctcggcgtcg ccggttgtac gttcatcatg ggggtgccgg gggccgacga
     2401 cgtcatgctc ggatatcaga gcacctcgtt tcacgacgcg ctgtatctgc gacgcgcgct
     2461 cgggctgcgg cccgcgccgg agttcgaggc ctggctcgag cgcgtcggcg tgttcgaccc
     2521 ggcgacgcac tcgctgcgcg gcgccccgcc ggccgcgctg ctgcgcgcga tggcggcgct
     2581 gccgggaggg gacgcgtgag cagcgacgaa cttcggcggg cggtcgcgcg ggtgacctcg
     2641 gcgcgggtgc tgctcggccg ggccggaccg gcgttgccga cccgcgcgtg gctcgagttt
     2701 cagctcgcgc acgcccgggc ccgcgacgcg gtgctcgctc cctgtgacct cgagggcctc
     2761 gcgagtgcgt tgcgggcgcg cgcggtcgag gcggtcgtcg tgcgcagc
//
LOCUS       DASWNJ010000215         4274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_215360, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000215 DASWNJ010000000
VERSION     DASWNJ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215360"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VGB85_03965"
     CDS             complement(<1..364)
                     /locus_tag="VGB85_03965"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0133204.1"
                     /translation="MSLSDVRRRLPVVTSLPSPGNRWSRPDAPIKGPRWAIWELTLAC
                     DQKCAHCGPRAGHRRPDELTTEECLKVVQELRELGCGEVVLIGGEAYLRNDFILIIRA
                     IREAGMSCTMTTGAYNLNP"
     gene            complement(429..2468)
                     /locus_tag="VGB85_03970"
     CDS             complement(429..2468)
                     /locus_tag="VGB85_03970"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY0133205.1"
                     /translation="MLDNPLLQIPLRVPFDRITPEHVEPAITRLLAEARATIQAIVEE
                     AGPRTYANTLGALDGATEALERAMTVVGHLEGVTTTPELRAAYNAVQGPVAEFSAQIP
                     LDSGLWQALRAYADTEDARALTGARLRFLTKTLDDFRRHGAELPPEGKARLSEIGVAL
                     STTTTEFSQHVLDATNAVDMVLTDPAELEGLPPSAVAAARASAEARDQEGWRFTLQQP
                     SYVAFMTYAARGDLRERLYRAANICATPGPHDNRPLLAEILRLRHEQATLLGFRDFPD
                     FVLHDRMARRGETAREFVARLRDKVRAAFVAENHSLAEFRRSLEGPDAPPLQPWDVAY
                     YAEKQRQALYEVDDEALRPYFLAPAVLAGLFEVVQRLYGVRVTATDDFPTWHPDVRTY
                     AVDDEHGVRLGYFYADLYPRDTKNAGAWMNAFITGAPDGRGGFGPHLGLIVGNLTPPV
                     GDAPALLKHDEVETLFHEFGHLLHHLLTRVEVRSLAGANVAWDFVELPSQIMENWCWE
                     REALDLFARHWQTGESLPEALLAPMQRARNFRAANATMRQLGFAATDLELHTGAPPPD
                     GDAVLARAREIMADYAPTPLPDDFAMLARFTHLFAGPVAYASGYYSYKWAEVLDADAF
                     SRFKREGVLSRAAGDAFRETILSKGDSREPAELFRAFMGRDPDPDALLARAGLLT"
     gene            2567..>4274
                     /locus_tag="VGB85_03975"
     CDS             2567..>4274
                     /locus_tag="VGB85_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY0133206.1"
                     /translation="MLRSDPEASATITALLGPTNTGKTHQAITRLLEHRTGMIGLPLR
                     LLAREVYDRISAQVGESAVALITGEEKRVPARPRYWVCTVESMPTSQPVDFMAIDEIQ
                     LAAHRERGHVFTDRLLHARGERETMFLGSDTMTEIVQAVVPTVRVERHPRFSRLKNLP
                     PSELAMLPPRSAVVAFSAAQVYELAEKLRRRRGGAAVVLGALSPRTRNAQVAMYQAGE
                     VQYMVATDAIGMGLNMDVDHVAFASLTKFDGKVLRPLEPAELAQIAGRAGRYTKDGTF
                     GVLAGVPAIPPGMVQQIEGHRFAPIDRLIWRSSEFDFSSLDALQASLKQRPRDPHLRL
                     VERADDADALAELAQFPEIRQFATGPERVSLLWDVCRIPDFRKLLEGSHIRLLANVFT
                     QLARDGRLDPGWMDRRIKHLDNDEGDIEALMSRISFVRTWTYISNHSRWISDPEHWQA
                     QTRAIEDRLSDALHERLTQRFVDPRARSVFVAPTPARSKKPATALAATPAQVRAASPD
                     NPFRQLLALRLADTEAPQGPLEDEAARIIEAPHEAFELDARGQIRLGEMAVATMTRGA
                     DLL"
BASE COUNT          619 a         1419 c         1647 g          589 t
ORIGIN      
        1 cggggttgag gttgtaggcg ccagtcgtca tcgtgcacga catgccggcc tcgcggatcg
       61 cccggatgat caggatgaaa tcgttgcgca ggtaggcctc gccgccgatc agcacgacct
      121 cgccgcagcc gagctcacgc agctcctgga cgaccttgag gcactcctcg gtggtcagct
      181 cgtcggggcg ccggtggccc gcgcgcggac cacaatgcgc gcacttctga tcgcaggcga
      241 gggtcagctc ccagatcgcc caccgcgggc ccttgatggg cgcgtcgggg cggctccagc
      301 ggttgccggg cgagggcagg gaagtgacaa cgggcaagcg gcgacgaacg tcggacagcg
      361 acacgccggc acgctaacat ggatccggca catcgatcgc ggcgcgatcg atgcacctgt
      421 ccctcgggtc aggtcagcag gccggcgcga gcgagcagcg cgtcggggtc ggggtcgcgg
      481 cccatgaagg cgcggaacag ctcggcgggc tcgcggctgt cgcccttgct gaggatcgtc
      541 tcacggaagg cgtcgccggc cgcgcggctg agcacgccct cgcgcttgaa gcgggagaag
      601 gcgtcggcgt cgaggacctc ggcccacttg tacgagtagt agccggacgc gtaggcgacc
      661 ggaccggcga acaggtgggt gaagcgggcg agcatagcga agtcgtccgg cagcggggtc
      721 ggggcgtagt cggccatgat ctcgcgggcg cgggccagca cggcgtcgcc gtctggcggc
      781 ggggcgccgg tgtgcagctc gaggtcggtc gccgcgaagc cgagctggcg catcgtcgcg
      841 ttggcggccc ggaagttgcg cgcgcgctgc atcggcgcga gcagggcctc tggcagcgac
      901 tcaccggttt gccagtggcg cgcgaacagg tcgagcgcct cgcgctccca gcaccagttc
      961 tccatgatct gcgagggcag ctcgacgaag tcccaggcca cgttggcgcc ggcgaggctg
     1021 cgcacctcga cgcgcgtcag caagtgatgc agcaggtggc cgaactcgtg gaacagggtc
     1081 tcgacctcgt cgtgcttgag cagggccggg gcgtcgccca ccggaggcgt aaggttgccg
     1141 acgatgaggc ccaggtgcgg gccgaagccg ccgcggccgt ccggggcgcc ggtgatgaag
     1201 gcgttcatcc acgcgccggc gttcttggtg tcgcgcgggt agaggtcggc gtagaagtag
     1261 ccgaggcgca cgccgtgctc gtcgtcgacg gcgtaggtgc gcacgtcggg gtgccaggtc
     1321 gggaagtcgt cggtcgcggt gacgcggacg ccgtagaggc gctgcacgac ctcgaacagc
     1381 ccggcgagca cggccggggc caggaagtag gggcgcaggg cctcgtcgtc gacctcgtag
     1441 agggcctggc gctgcttctc ggcgtagtag gcgacgtccc agggctgcag cggcggggcg
     1501 tcggggccct cgagcgagcg acgaaactcg gcgaggctgt gattctcggc gacgaaggcg
     1561 gcgcggacct tgtcgcgcag gcgggcgacg aactcgcgcg cggtctcgcc acggcgggcc
     1621 atgcggtcgt gcaggacgaa gtcggggaag tcgcgaaagc cgagcagggt cgcctgctcg
     1681 tgacgcaggc gcaagatctc cgccaggagc gggcggttgt cgtggggccc gggcgtcgcg
     1741 cagatgttgg cggcccggta caggcgttcg cgcaggtcgc cgcgcgcggc gtaggtcatg
     1801 aaggcgacgt agctcggctg ctgcagcgtg aagcgccagc cctcctggtc gcgggcctcg
     1861 gcgctggcgc gcgcggcggc gaccgccgag ggcggcaggc cctcgagctc ggcggggtcg
     1921 gtgagcacca tgtcgacggc gttggtcgcg tcgagcacgt gctgcgagaa ctcggtggtg
     1981 gtggtgctca gcgcgacgcc gatctccgac agccgggcct tgccctcggg cggcagctcg
     2041 gcgccgtggc ggcggaagtc gtcgagggtc ttggtgagga agcgcaggcg ggcgccggtg
     2101 agggccctgg cgtcctcggt gtcggcgtag gcgcgcaggg cctgccacag gccgctgtcg
     2161 agcgggatct gggcggagaa ctcggcgacg gggccctgca cggcgttgta ggcggcgcgc
     2221 agctccgggg tggtcgtcac gccctcgagg tggccgacga cggtcatggc gcgctcgagg
     2281 gcctcggtcg cgccgtcgag ggccccgagg gtgttggcgt aggtccgggg cccggcctcc
     2341 tcgacgatcg cctggatggt ggcgcgggcc tcggcgagca gccgggtgat ggcgggttcc
     2401 acgtgctcgg gggtgatgcg gtcgaacggg acccggaggg ggatctggag gagggggtta
     2461 tccagcacag acgccaagat agcaggcccg acgtgcatcg gcgggcctcc cgtggcatgg
     2521 atcggtgcgc cggccggcgg cgctcctcgg ccccggatct tccatcatgt tgcgcagcga
     2581 ccccgaagcc tccgcgacga tcacggcgct gctcggcccg accaacaccg gcaagacgca
     2641 ccaggcgatc acccggctgc tcgagcaccg caccggcatg atcggcctgc ccctgcgcct
     2701 gctcgcgcgc gaggtctacg accgcatctc ggcgcaggtc ggcgagtccg cggtcgcgct
     2761 gatcaccggc gaggagaagc gcgtgccggc gcgcccgcgc tactgggtgt gcacggtcga
     2821 gtcgatgccg acctcgcagc cggtcgactt catggcgatc gacgagatcc agctcgcggc
     2881 ccaccgcgag cgcgggcacg tgttcaccga tcgcctgctg cacgccaggg gcgagcgcga
     2941 gacgatgttc ctcggctccg acacgatgac ggagatcgtc caggcggtcg tgccgacggt
     3001 ccgcgtcgaa cgtcacccgc gcttctcgcg gctaaaaaac ctcccgccga gcgagctcgc
     3061 gatgttgccg ccgcgctcgg cggtggtcgc gttctcggcc gcgcaggtct acgagctggc
     3121 ggagaagctc cgccggcgac gcggcggcgc ggcggtggtg ctcggggccc tgtcgccgcg
     3181 cacgcgcaac gcccaggtcg cgatgtatca ggcgggcgag gtccagtaca tggtagcgac
     3241 cgacgcgatc ggcatgggcc tcaacatgga cgtcgaccac gtcgcgttcg cgtcgctgac
     3301 gaaattcgac ggtaaggtat tgcggccgct cgagccggcc gagctggcgc agatcgccgg
     3361 ccgggcgggt cgatacacca aggacggcac cttcggcgtg ctcgcgggcg tgcccgcgat
     3421 cccgccgggc atggtccagc agatcgaggg tcatcgattc gcgccgatcg accgcctgat
     3481 ctggcgcagc agcgaattcg acttcagcag cctcgacgcg ttgcaggcct cgctcaagca
     3541 gcgcccgcgg gatccgcacc tgcggctcgt cgaacgggcg gacgacgccg acgcgctcgc
     3601 cgagctggcg cagttcccgg agatccgcca gttcgcgacc ggcccggagc gcgtatcgct
     3661 gctgtgggac gtgtgccgga tccccgactt tcgcaagctg ctcgagggca gtcacatccg
     3721 gctgctcgcc aacgtgttca cgcagctggc ccgcgacggt cggctcgacc cggggtggat
     3781 ggaccgccgg atcaagcacc tcgataatga tgagggcgac atcgaggcgc tgatgagccg
     3841 gatctcgttc gtgcgaacgt ggacgtacat cagcaatcac agccgctgga tctccgaccc
     3901 ggagcactgg caggcgcaga cgcgggcgat cgaggatcgc ctcagcgacg cgctgcacga
     3961 gcggctgacc cagcgcttcg tcgacccgcg ggcccgcagc gtgttcgtgg cgccgacccc
     4021 cgcgcgcagc aagaagccgg cgacggccct ggccgcgacg ccggcgcagg tgcgcgcggc
     4081 ctcgccggac aacccgttta gacaactgct cgcgctgcgg ctcgccgaca cggaggcgcc
     4141 gcaaggtccg ctcgaggacg aggcggcgcg catcatcgag gcgccgcacg aggccttcga
     4201 gctcgacgcg cgcgggcaga tccggctcgg cgagatggcg gtggcgacga tgacccgcgg
     4261 cgccgacctg ctgc
//
LOCUS       DASWNJ010000216         5692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_498078, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000216 DASWNJ010000000
VERSION     DASWNJ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498078"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2821
                     /locus_tag="VGB85_03980"
     CDS             <1..2821
                     /locus_tag="VGB85_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0133207.1"
                     /translation="DDAWTTHYALCFALHCERMECEHLAGDIPAALARFEPLLDHARD
                     DLDRGEIHDLKVRLDVTRECHLEATRTGLAGLRLLGIDLPERATQSTVMRELAGVRWH
                     RGLRPFAALAGLPEVRDPKIRLALRLLARINIPAYYLDEPTLAAVALRCVALSIRHGV
                     SSDSAVGFAVYAAIALNRLEDPAAARDLWAVAHALGERFPSPALAVRMATFGGIFVEA
                     WVQPFIVATTHISGQIEPALALGDTTLAAMLMGAHIDLQWLGASTLAQIERTIAGVRP
                     VARRLPGFDHSHWFIPIERACLALRGRTDALASMSSADWQEHTLVDQLRPERVAPTFF
                     YAIYKAVLLIHAGRFREAELLALQYHRVRMEGAPLLAELLLLRILARTAQTPDRPLTS
                     ADRTHLHRWLRRMQLWVRACPENFAPRTLLAEAELARAEGQRDPATLLYMRAAAAARA
                     QGQLRIEGLAHERCGRHLLTGDTPGLSDMYFRAAREAYVRWGADAIVAALDAELPHLA
                     ALADPAPPVVDVRETTTTNPGALDVEALLRASQAISGEIELEPLLRTLVRIVQECAGA
                     RRCAVLLARGDGELRVEALAALDSVQVLQGLTLADARLPASVVHLAARSRRDVILDDA
                     AVSEFAGDLAVAGLRSVLCTPILHQGALLGVLYLDNELAPGIFTADRIALLRQLAGQV
                     AISVENARLYRNLAQARDAAVAADRTKTRFLMNMSHELRTPLNAVLGYTELIQESAAD
                     GDLTALHTDLDKIRRAANRLLQTLGSILELSRVEAGDLRPAPEPIDLEALVREVVEES
                     ASLAAAQDNQILVDIAADLPPLASDRGMLLHAVRSLLDNALRFTAGGRVELAIRGYLS
                     EGADWIAVRVQDTGIGIASADLPRLFTSFGQLDDAPTRNFEGTGVSLALTRRFCALLG
                     GRVEVESEPGTGSSFTLHLPASAPA"
     gene            2898..3830
                     /locus_tag="VGB85_03985"
     CDS             2898..3830
                     /locus_tag="VGB85_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133208.1"
                     /translation="MPRALLLPFALLLACTPEFHSVTASPATSDADDSDSDATPTGGP
                     ACVNQFDCPRLGVCEAVDCVDGECIYGDALVDDDPFDDVEPEGPSGDCKRMTCTAKGE
                     MTEVDDPNDAPEGENLCSLGTCDPGPKQQPAAPNEDCSGLLDSAPSYCWPDTSCHFCE
                     YVAPNECDADGDEPSETQETARDLGEVDDADLFEKYVRCATLGSASDVDWFKFHGSDS
                     LLGVIDPMRSLYDAGGGARLCVFFACDTGGTEVACAQGEPDTAPGGQPGCCGAGQVVP
                     TLTCADNYNYSADVWLRVDQGPAACTPYELRYNF"
     gene            3869..4900
                     /locus_tag="VGB85_03990"
     CDS             3869..4900
                     /locus_tag="VGB85_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY0133209.1"
                     /translation="MPPARLATLLVCLSLAPPALAAPAPVPEASQPDPRDSPTGYEPA
                     PAPVPESSPPPPPAPVAPVPEDIPSSTPPPPEPVVLAPVLAPPHPMRVRLIVDLPIIG
                     LSTAIALGSEYVGKEQRWAGCAACDRGEINALDRTVLGNHSAAAKTVSDIGLFSLIAL
                     PFALDLGDALIQRSRDRSPGRARHLRGWGKDVVVLLETFAVTYASTNVVKFAVRRPRP
                     YSYDADSGVADPTENDARLSFYSGHASMAFAMATSYAYLFQARHPRSRWVAPVWVIGM
                     SLASVTAVARVEAGKHFWTDVIVGAAAGTGIGLLIPALHRNRLLGERVGLSLAPTRTG
                     SSLLLQGRF"
     gene            5020..5505
                     /locus_tag="VGB85_03995"
     CDS             5020..5505
                     /locus_tag="VGB85_03995"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY0133210.1"
                     /translation="MDAIDLLERHHDELEKKFTALKDADKPSADDFKAASDQLVSHMT
                     IEEQLFYPAVKAKRTEDILLESLEEHLSLKRVLSDLLQLQPDDPKFGPKMHVLAEQLE
                     HHHHEEEDHLFPKVKKLLDSDAREELGARMQARLDELEHQAPRKLVHDQTDAAAPLPD
                     A"
     gene            5502..>5692
                     /locus_tag="VGB85_04000"
     CDS             5502..>5692
                     /locus_tag="VGB85_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133211.1"
                     /translation="MTVREPSPGGRVEDSGCFPEAGLDYRDMTPGERMEALRRLSRRA
                     YTCIPADDPRRLEGLPNRL"
BASE COUNT          787 a         2439 c         1716 g          750 t
ORIGIN      
        1 cgacgacgcc tggaccaccc actacgcgct gtgcttcgcg ctgcactgcg agcgcatgga
       61 gtgcgagcac ctcgccggcg acatcccggc cgcgctcgcc cgcttcgagc cgctcctcga
      121 ccacgcccgc gacgacctcg atcgcggcga gatccacgac ctcaaggtgc gcctcgacgt
      181 cacccgcgag tgtcacctcg aggccacccg caccggcctc gccggcctgc gcctgctcgg
      241 catcgacctg cccgagcgcg ccacccagtc gacggtcatg cgcgagctcg cgggcgtccg
      301 ctggcaccgc ggcctgcgtc ccttcgccgc cctcgccggc ctgcccgagg tccgcgaccc
      361 gaagatccgc ctcgccctgc gcctgctcgc gcgcatcaac atccccgcct actacctcga
      421 cgagccgacc ctcgccgccg tcgccctgcg ctgcgtcgcc ctctcgatcc gccacggcgt
      481 cagcagcgac tccgccgtcg gcttcgccgt ctacgccgcg atcgccctca accgcctcga
      541 agatcccgcc gccgcgcgcg acctgtgggc cgtcgcccac gcgctcggcg agcgcttccc
      601 gagccccgcc ctcgccgtgc gcatggcgac cttcggcggc atcttcgtcg aggcctgggt
      661 gcagcccttc atcgtcgcca ccacacatat ttcgggccag atcgagccgg ccctcgcgct
      721 cggcgacacc accctcgccg cgatgttgat gggcgcgcac atcgacctcc agtggctcgg
      781 cgccagcacc ctcgcgcaga tcgagcgcac gatcgccggc gtgcgaccgg tcgcccgccg
      841 cctcccgggc ttcgatcact cgcactggtt catcccgatc gagcgcgcct gcctggccct
      901 gcgcggccgc accgacgccc tcgccagcat gtcgagcgcc gactggcagg agcacaccct
      961 cgtcgaccag ctgcgccccg agcgggtcgc gccgacgttc ttctacgcga tctacaaggc
     1021 ggtcctgctg atccacgccg gccgcttccg cgaggccgag ctgctcgcgc tccagtacca
     1081 ccgcgtgcgc atggagggcg ccccgctgct cgccgagctg ctgctgctgc ggatcctcgc
     1141 gcgcaccgcc cagacccccg accggccgct gacctccgcc gaccgcaccc acctgcatcg
     1201 ctggctgcgc cgcatgcagc tgtgggtccg cgcctgcccc gagaacttcg ccccgcggac
     1261 cctcctcgcc gaggccgagc tcgcccgcgc cgagggccag cgcgaccccg ccacgctcct
     1321 gtacatgcgc gccgccgcgg ccgcgcgcgc gcagggccag ctgcggatcg agggcctcgc
     1381 gcacgagcgc tgcggccggc acctgctcac cggcgacaca cctggccttt cggacatgta
     1441 ctttcgcgcc gcccgtgagg cctacgtccg ctggggcgcc gacgcgatcg tcgccgccct
     1501 cgacgccgag ctcccgcacc tcgccgccct cgccgacccg gcccccccgg tcgtcgacgt
     1561 ccgcgagacg accaccacca accccggcgc cctcgacgtc gaggccctgc tgcgcgcctc
     1621 ccaggcgatc tccggcgaga tcgagctcga gccgctgctg cgcaccctcg tccgcatcgt
     1681 ccaggagtgc gccggggccc gtcgctgcgc cgtgctgctc gcccgcggtg acggcgagct
     1741 gcgcgtcgag gccctcgccg cgctcgacag cgtccaggtc ctgcagggcc tcaccctcgc
     1801 cgacgcccgc ctgccggcct ccgtcgtgca cctcgccgcc cgcagccgcc gcgacgtcat
     1861 cctcgacgac gcggccgtca gcgagttcgc cggcgacctc gccgtcgccg gcctgcgctc
     1921 cgtcctgtgc accccgatcc tgcatcaggg cgcgctgctc ggcgtgctct acctcgacaa
     1981 cgagctcgcg cccggcatct tcaccgccga ccgcatcgcc ctgctgcgcc agctcgccgg
     2041 ccaggtcgcc atctcggtcg agaacgcccg gctctaccgc aacctcgcgc aggcccgcga
     2101 cgcggccgtc gccgccgacc ggaccaagac ccgcttcctg atgaacatga gccacgagct
     2161 gcgcaccccg ctcaacgccg tgctcggtta caccgagctg atccaggagt ccgcggccga
     2221 cggcgacctc accgcgctgc acaccgacct cgacaagatc cgccgcgccg ccaaccgcct
     2281 gctgcagacg ctcggcagca tcctcgagct ctcgcgcgtc gaggccggcg acctgcgccc
     2341 cgcgcccgag ccgatcgacc tcgaggcgct cgtgcgcgag gtcgtcgagg agtccgccag
     2401 cctcgccgcc gcgcaggaca accagatcct cgtcgacatc gccgccgacc tcccgcccct
     2461 cgccagcgac cgcggcatgc tgctgcacgc cgtgcgcagc ctcctcgaca acgccctgcg
     2521 cttcaccgcc ggcggccgcg tcgagctcgc catccgcggc tacctgtccg aaggggcaga
     2581 ctggatcgcc gtccgcgtgc aggacaccgg catcggcatc gcctccgccg acctcccgcg
     2641 cctgtttacc agcttcggtc agctcgacga cgcgccgacc cgcaacttcg agggcaccgg
     2701 cgtcagcctg gccctgacgc gccgcttctg cgccctgctc ggcggccgcg tcgaggtcga
     2761 gagcgagccc ggcaccggca gcagcttcac cctgcacctg cccgccagcg cgcccgcgtg
     2821 atcgcatgat caagtgtacg tgcgcggtga cccccagcac cgcccggccg accgcccgcc
     2881 ggtatcgtgc accaccgatg ccccgcgccc tgctcctgcc gttcgcgctc ctgctcgcct
     2941 gcacacccga attccattcg gtcaccgcga gcccggccac cagcgacgcc gacgacagcg
     3001 acagcgacgc caccccgacc ggcggccccg cgtgtgtcaa ccagttcgac tgcccgcgcc
     3061 tcggcgtatg cgaggccgtc gactgcgtcg acggcgagtg catctacggc gacgccctcg
     3121 tcgacgacga ccccttcgac gacgtcgagc ccgagggccc gtccggcgac tgcaagcgga
     3181 tgacctgcac cgcgaagggc gagatgaccg aggtcgacga cccgaacgac gcgccggagg
     3241 gcgagaacct gtgctcgctc ggcacctgcg atccgggccc gaagcagcag cccgcggccc
     3301 ccaacgagga ctgcagcggc ctcctcgaca gcgccccctc gtactgctgg cccgacacca
     3361 gctgccactt ctgcgagtac gtcgcgccga acgagtgcga cgccgacggc gacgagccca
     3421 gcgagaccca ggagaccgcg cgcgacctcg gcgaggtcga cgacgccgac ctcttcgaaa
     3481 aatatgtgcg ctgcgcgacc ctcgggtccg ccagcgacgt cgactggttc aagtttcacg
     3541 gcagcgacag cctgctcggc gtgatcgacc cgatgcgctc gctgtacgac gccggcggcg
     3601 gcgcacgcct gtgcgtcttc ttcgcctgcg acaccggcgg caccgaggtc gcgtgtgcac
     3661 agggcgagcc cgacaccgcc cccggcggcc agcccggctg ctgcggcgcc ggccaggtcg
     3721 tccccaccct cacctgcgcc gataactaca actacagcgc cgacgtctgg ctgcgcgtcg
     3781 accagggccc cgccgcctgc accccctacg agctgcgcta caacttttga gccgaagcga
     3841 ccgcgttcgg tttagcctgc tgcccggcat gccgcccgct cgcctcgcca cgctcctggt
     3901 ctgcctgtcc ctcgcgcccc cggccctcgc cgcgcccgca cctgtccccg aggccagcca
     3961 acctgacccc agggacagcc cgaccggcta cgagccggcg cccgcgcccg tcccggagag
     4021 cagcccgccg ccgccgcccg cgcccgtcgc acctgtcccc gaggacatcc cgtcttccac
     4081 gccaccgccg cccgagcccg tcgtcctcgc gcctgtcctc gcgccaccgc acccgatgcg
     4141 cgtgcgcctg atcgtcgacc tcccgatcat cggcctctcg accgcgatcg ccctcggcag
     4201 cgagtacgtc ggtaaggagc agcgctgggc cggctgcgcc gcctgcgatc gcggcgagat
     4261 caacgcgctc gaccgcaccg tgctcggcaa ccacagcgcc gccgcgaaga ccgtctccga
     4321 catcggcctg ttctccctga tcgccctgcc cttcgcgctc gacctcggcg acgccctgat
     4381 ccagcgcagc cgcgatcgtt caccgggacg cgcgcgtcac ctccgcggct ggggcaagga
     4441 cgtcgtcgtc ctgctcgaga ccttcgccgt cacctacgcg agcaccaacg tcgtcaagtt
     4501 cgcggtccgg cgcccgcgcc cgtacagcta cgacgccgac agcggcgtcg ccgacccgac
     4561 cgagaacgac gcccgcctgt cgttctactc cggccacgcc tcgatggcct tcgccatggc
     4621 gaccagctac gcctacctgt tccaggcccg tcacccgcga tcgcgctggg tcgcgcccgt
     4681 gtgggtgatc ggcatgagcc tcgccagcgt caccgccgtc gcccgcgtcg aggccggcaa
     4741 gcacttctgg accgacgtca tcgtcggcgc cgccgccggc accggcatcg gcctgctgat
     4801 ccccgccctg caccgcaacc gcctgctcgg cgagcgcgtc ggcctcagcc tcgcccccac
     4861 gcgcaccggc agctcgctgc tgcttcaggg ccgcttctga cactcgcgca gcctccgcgc
     4921 tttgctcgct gcgacctcga cgacggccac tgcgcccgcg ccccgacgcg gccgcccccc
     4981 ggcgagcgcg cgcggcgaag cgcgctagag aaccggcaca tggacgccat cgacctcctc
     5041 gaacgccacc acgacgagct ggagaagaaa ttcacggccc tcaaggacgc cgacaagccg
     5101 agcgccgacg acttcaaggc cgcctccgac cagctcgtca gccacatgac gatcgaggag
     5161 cagctgtttt accccgcggt caaggccaag cgcaccgagg acatcctgct cgagtcgctc
     5221 gaggagcacc tctcgctcaa gcgcgtgctc tccgacctcc tgcagctcca gcccgacgac
     5281 cccaagttcg gccccaagat gcacgtgctc gccgagcagc tcgagcatca ccaccacgag
     5341 gaggaggacc acctgttccc caaggtcaag aagctgctcg acagcgacgc ccgcgaggag
     5401 ctcggcgccc gcatgcaggc caggctcgac gagctcgagc accaggcccc ccgcaagctc
     5461 gtccacgacc agaccgacgc cgccgccccg ctccccgacg catgaccgtc cgcgagcctt
     5521 ctccgggtgg tcgtgtcgag gactcgggct gcttcccgga ggccggcctc gactaccgcg
     5581 acatgacccc gggcgagcgc atggaggcgc tgcgccgcct gagccgtcgc gcatacacgt
     5641 gtattccggc cgacgacccg cgccgactcg agggactgcc gaaccgcctc at
//
LOCUS       DASWNJ010000217         2562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_251882, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000217 DASWNJ010000000
VERSION     DASWNJ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251882"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2562
                     /locus_tag="VGB85_04005"
     CDS             <1..>2562
                     /locus_tag="VGB85_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HEY0133212.1"
                     /translation="SLRSGECDMALAGGSSVTSPQATGYLYREGMITSRDGHCRAFDA
                     EASGTVFGSGVGVVLLKRHRDALADGDTIYAVIRGSATNNDGASKLGYAAPSGEGQAA
                     VVAEALAVAGVDAGTIGFVEAHGTATPFGDPIEVEALSQAFRSHTDRRQYCALGSVKT
                     NVGHLQITSGIAGFIKAVLALYHRTIPPTLHFRVANPEIDLGNSPFFVNTEVAAWPEA
                     SDPPRASVHSLGIGGSNVHVVLEAAAQVTPGPAAEERTAHVCTLSARSEAALATRIEQ
                     LAAHVSAHPELPLADVCYSANAGRSHWRHRAAVVASTSEQLVARLAGLGAGESGPVGG
                     PPSVAFLFSGQGCQYGGMARGLYATHPGFRADMDRSEEVFRKRTGQSLLAVMFADHDP
                     RLHHTAFTQPALFAVEVALAGLWRSWGVEPAAMLGHSVGMYAAACTAGVLSLADGMAL
                     VTERARLMGALPEGGTMAAVFADPAEVRALLRDFAGRVHVAAVNAAQQVVISGETAAV
                     AAVREVLDRKGVRSQPLHVSHAFHSHLMRPMLAEYRQVLSEVLWQPATIPLIADLTGA
                     PADDTLAGPDYWQRHIVQPVLFHDGMLALRRAGCTVFVEIGPKPSLLALSEVTREPDT
                     LAIASLRRDGDDWAVLLAGLARLYTRGVALDWQAFEAPYRRRRVPLPTYPFERHRHWL
                     DPVEPGDVASGGGQPLLGRRITVASPERFFDATLTPSSHAYLWEHQVFGAAILPLAAH
                     LELAWEAAEHTGTTADAALVIEDLAVHRPLLLGHTRCVVHAKLGPASDGPVPLEILLA
                     RDGRWESIATSRVSHQRVAAARLERPALARRCPAPGDPATLYADFADRGIDYGPI"
BASE COUNT          315 a          964 c          930 g          353 t
ORIGIN      
        1 gagtctgcgc agcggcgagt gcgacatggc gctcgctggc ggctcgtcgg tgacgtcgcc
       61 gcaggcgacc ggctacctct accgcgaggg catgatcacc tcgcgcgacg gacactgccg
      121 ggcgttcgac gccgaggcgt cggggacggt gttcggcagc ggcgtcggcg tcgtcctgct
      181 caagcggcac cgcgacgcgc tcgccgacgg cgacacgatc tacgccgtga tccgcggctc
      241 cgcgaccaac aacgacggcg cgtccaagct cggctacgcc gcccccagcg gcgaggggca
      301 ggccgcggtc gtcgcggaag cgctggcggt cgcgggcgtc gacgccggca cgatcggctt
      361 cgtcgaggcc catggcacgg cgactccgtt cggcgatccg atcgaggtcg aggcgctgtc
      421 ccaggcgttc cggtcacaca ccgaccgccg gcaatattgc gcgctcgggt cggtcaagac
      481 caacgtcggc cacctccaga tcacgtccgg catcgccggg ttcatcaagg ccgtgctggc
      541 cctgtatcac cgcacgatcc cgccgacgtt gcactttcgc gtggcaaacc ccgagatcga
      601 cctcggcaac agccccttct tcgtcaacac cgaggtcgcg gcgtggccgg aggccagcga
      661 tccgccgcgt gccagcgtgc actcgctggg gatcggcggc agcaacgtgc acgtggtcct
      721 cgaggcggcc gcgcaggtga ccccagggcc ggccgccgag gagcggacgg cccacgtctg
      781 cacgctgtcg gcgcgcagcg aggcggcgct cgccacccgc atcgagcagc tcgccgccca
      841 tgtatctgcg caccccgagc ttccgctcgc cgacgtgtgc tactcggcca acgccggccg
      901 cagccactgg cggcaccgcg ccgcggtcgt cgcgagcacg agcgagcagc tggtcgcgcg
      961 gctggcgggg ctcggcgccg gcgagtcggg acccgtgggc gggccgccgt cggtcgcgtt
     1021 cctgttctcg ggccagggct gccagtacgg cggcatggcg cgcggcctgt acgcgacgca
     1081 cccggggttc cgcgccgaca tggaccgcag cgaggaggtg tttcgcaagc ggaccgggca
     1141 gtcgctgctc gcggtcatgt tcgccgacca cgatccacgg ctgcaccaca ccgcgttcac
     1201 ccagccggcg ctgttcgccg tggaggtcgc gctcgccggt ctgtggcgga gctggggcgt
     1261 cgagcccgcg gcgatgctcg gccacagcgt cggcatgtac gccgccgcct gcacggcggg
     1321 cgtgctctcg ctggccgacg ggatggcgct ggtgaccgag cgcgctcgcc tcatgggcgc
     1381 gctcccggag ggcggcacga tggcggcggt gttcgccgac ccggccgagg ttcgtgcgct
     1441 gctccgcgac ttcgccgggc gcgtccacgt ggcggccgtc aacgcggccc agcaggtcgt
     1501 gatctccggc gagacggcgg ccgtggcggc cgtgcgggag gtgctcgacc gcaagggcgt
     1561 gcgtagccag ccgctccacg tgtcgcacgc gttccactcg cacctgatgc gcccgatgct
     1621 cgccgagtac cggcaggtgc tgtccgaggt gctctggcag cccgcgacca tcccgttgat
     1681 cgccgacctc accggagccc ccgccgacga cacgctcgcc gggcccgact actggcagcg
     1741 gcacatcgtc cagccggtgc tgttccacga cggcatgctc gccttgcgcc gcgccggctg
     1801 caccgtgttc gtggagatcg gccccaagcc cagcctgctc gcgctgagcg aggtgacgcg
     1861 cgagccggat acgctggcga tcgccagcct gcggcgtgac ggcgacgact gggcggtgct
     1921 gctcgccggc ctggcgcgcc tgtacacgcg cggcgtcgcg ctcgactggc aggccttcga
     1981 ggcgccgtat cgccgccggc gggtcccgct gccgacctac ccgttcgagc gccaccggca
     2041 ctggctcgac ccggtggagc ccggcgacgt cgcgtcgggt ggcggccagc cgctgctcgg
     2101 tcggcggatc accgtggcga gcccggagcg gttcttcgac gcgaccttga ccccgagctc
     2161 gcacgcgtac ttgtgggagc accaggtgtt cggcgcggcg atcttgccgc tcgcggctca
     2221 cctcgagctc gcgtgggagg cggccgagca caccggcacg accgccgacg cggccctggt
     2281 gatcgaggac ctcgcggtgc accgcccgct gctgctcggc cacacgcgct gcgtcgtgca
     2341 cgcgaagctg ggccccgcga gcgacggtcc ggtcccgctc gagatcctgc tcgcgcggga
     2401 cggccgctgg gagtcgatcg ccaccagccg cgtgtcgcat cagcgcgtcg ccgcggcccg
     2461 cctcgagcgc ccggcgctgg cccggcgctg cccggcgcca ggcgacccgg cgacgctgta
     2521 cgccgacttc gccgaccgcg ggatcgacta cggcccgatc tt
//
LOCUS       DASWNJ010000218        10252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_75418, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000218 DASWNJ010000000
VERSION     DASWNJ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 10252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10252
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75418"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..396)
                     /locus_tag="VGB85_04010"
     CDS             complement(127..396)
                     /locus_tag="VGB85_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133213.1"
                     /translation="MDEDLRAELIRWIAEDDETRERLARDGSLFDGYHPEMEAVHRRN
                     AARLREVLARVGWPGRALVGDDGASAAWRIVQHAIGEPELLRGCL"
     gene            complement(671..1519)
                     /locus_tag="VGB85_04015"
     CDS             complement(671..1519)
                     /locus_tag="VGB85_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133214.1"
                     /translation="MTRSLMQTAIRPEVGSTLRVTRVCLRADAPYGTRVEDTPWGTLV
                     GLATGSESGWDCGARTLGSPALSLMFDLLRGPDPVAALPGAFAAGSQCFAADPSFAEN
                     DEPDLPILTVVLALCEPRRITLAWVGNDKAYLLRDGRVAAQSEEHTRRMLLRGPLARP
                     LTPTEAAPDEHDIAWRALPAEQRLLGPIRRLFADLPTLVLSRDVRGPPAMMHLERRPG
                     DMLLLLAGGLHKRLEWDAGLTAALAPGPLTIDRLVTEIDRRDPRRTGELAGGSDPYGP
                     ILLAEL"
     gene            complement(1558..3090)
                     /locus_tag="VGB85_04020"
     CDS             complement(1558..3090)
                     /locus_tag="VGB85_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133215.1"
                     /translation="MVVELGSQRLLVLLALAGCGASGAGTDETGTSSTSTGSTGDAPQ
                     STGVEGTDTPTPTTGEPTTSEPTTETTGTPIDACAGVINEPGTVVDLAWPAAENVGHD
                     TIAGQTVRFHWTGLHNVLQVATFAGQIPPTAKYGDAGWPGQIRSGDKQVDGVFEWHTG
                     SFPCGYRPGIYFFVDQDDPAGGVVSVPVTVPEFENDHYEPRPCTVLADPLVYEGRYAQ
                     YADRPGCTVFEVNNFQTVAHYDWVDPVLLARQGDLILFRWTGEHNIVQVHDVLQDELV
                     PGGLHSGPKTNCVGGPNYSCANGSPDLGEYLVDTTDRRPGIVHISDECAYKCTGHETG
                     MNMQFDLRFPVPTNTPLPPVAGSCCAIDPSRGADCRVVEIYNGNDGAQLDYNVPIGAH
                     DLVRFRWAGSLRIYQSLLDGNGAPTTSAKPGGIAMDAAVECIPGPNMTCLQGTPEQAQ
                     LVFDVAAELAADNFEPGMFGERFFAFAAEGENTPGFTSADSKTLLYVDDSVPHDPGNA
                     CP"
     gene            3375..3815
                     /locus_tag="VGB85_04025"
     CDS             3375..3815
                     /locus_tag="VGB85_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0133216.1"
                     /translation="MEEPSQPDAQPAGTNTRSLTMSGVYLDPELVRLCGITTPTALFE
                     FDSAAVEGADNSGLLAVATCVSTGPLKGRQIQLIGHTDPRGTDDHNTQLGKSRADAVM
                     QFLANEGGVPADRMTTLSEGEKGADPTNADGWSYDRRVDIRLVP"
     gene            complement(3838..5307)
                     /locus_tag="VGB85_04030"
     CDS             complement(3838..5307)
                     /locus_tag="VGB85_04030"
                     /inference="COORDINATES: protein motif:HMM:NF025714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HEY0133217.1"
                     /translation="MTKLRGVLLLVLVLVLGGAGYFAWRYKHRVQPEPPPRGASLPGA
                     PPTASQSSVSVPIAVDAALIEGIANDKLPHDLLARPGIAAGEGVTAGVVVRRGGRITA
                     GAEAGKLHLRVPIEADIEADWQPAGLVGLMSRGKRQHIKTRATFVVRAEIDLGVDAQW
                     NLATSTEATLRWEDDPVVPVGPLNVKLSALVGDRIDEQFAGVTRTLDEQLRRQIPTRP
                     LITQAWAAAFRTLRVGPRGDLWLVLRPTAMYLGEVRAHDGQVSLDAAIHGVFRVVVGA
                     EPPAAEPTPLPARSAPPGEPGVALDVPVSLTFAAANQQLDERLEGQVFDVPLDVVGQS
                     VALTIGAVEVYPSGEHVAVAVEFSADLPGAWLDMHGLVYLVGTPTLDPGRNQLSITDL
                     AYDSRTNVALVDVAEWMLHDAIVRRVRERLVFDFTGQLDGYRDQINAAIADYRISELI
                     RLRGKIDEVRVVAVTVTDPAIVAGVQLRGSAKLDVAPSP"
     gene            complement(5566..6714)
                     /locus_tag="VGB85_04035"
     CDS             complement(5566..6714)
                     /locus_tag="VGB85_04035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0133218.1"
                     /translation="MSSTFERLFMLSTAMMATIDPQGRFVALNPAWEKTLGWTLDELR
                     ALTPIELIHPDDRPAALAAAERMTRGRPVQLENRCRCQDGSYKWLMWSCSVDDALPPD
                     ERVWYASAVDISAHRETVRHNEQALARLRLFEAMLENTPDQVGIIDVQGRVLYRNASA
                     RRIVHAWGHPEGSTKITVKHSPAFTERLEREIRPILRAGGTWSGEGDLLLEDGSVMPI
                     YQVLVTLRDVDGAIVGWGSLIRDISDVKQIEQRLRAAVQDLATPLIPISDRVLVMPLI
                     GQIDVERASQVTRAALDGVHGRGVEVVLIDVTGLQQIDAEVAKLLLATTRALALLGVR
                     TVLTGIQPTVARTWIDLGLDLSGIETASTLQSAIAGALARRHSRPTAR"
     gene            7047..8879
                     /locus_tag="VGB85_04040"
     CDS             7047..8879
                     /locus_tag="VGB85_04040"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEY0133219.1"
                     /translation="MKRTNVADVEAKEVVHATATTPLQLLASADARPPHGGLLQLQGL
                     IGNRAVARLRGGSRDAALSHQVAAAGIRGVGRPLPHLEQIQAAFGGHDVRQVRAFTGG
                     AAATTSEALGAEAYATRERIAFRSAGPSLHTTAHEAAHIIQQRRGIHLPGGFGRAGDS
                     HERHADLVADRVVRGQSAESLLGRPIGPGSFASPTVQRAPLSARDLGRLYAAAEMDQT
                     NFTRLVSHWAKETGGAAKVRHEVKDMGRLAQKVREKAREDGIEEDVAIGRMYDVLGGT
                     IVYKNLDDFFAGYNKLKDELAPAARATIVRLKNRITQKNNRDFLINIKLESGFIVELQ
                     LHLEATLAGKQGDPVKVKADRERQGGFGRYTGHDAYDYTRILEGFFGAVARQTAGNQR
                     QIVLPGRKISPENRRRMATNRNLAQAQGDQRELAAIKADLRRPFVQKDLNKYYEELTT
                     LYNQIQDEITANAWEEVLSKGRGRLNDIEGIHSIDTLAEQDLGAIGEHWKANGDTRAL
                     INTRYRKFLSEADAHANAAAAGPASMLATFRMYYNILNEKHEVKKKKKANQKVPAYHA
                     KIDTYLKTMASAIKILEAQEEQRLQLAPQQPQQLPQQQAPAQPQ"
     gene            9191..10120
                     /locus_tag="VGB85_04045"
     CDS             9191..10120
                     /locus_tag="VGB85_04045"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEY0133220.1"
                     /translation="MKIVDLSLFANLFVVGCIDDGQAAPDDVADAEDVEELEDIEDAP
                     AGELDGLQALAPEPGSSVWSEVLLTDGTTQQFMLHTTLAGEVVFEELSDERFPQAPDL
                     TTLPEQAAALGACSDDAYILQGWRWKSALRWSFHAGSTPDELTVDAAEAAITAATASI
                     TSARNSCGLADEVDASHEYLGRKHKGANIGASGNCLTSDGDNVVSFGDLPQGVLATAC
                     VWFHDGVATEGDVQLNKQDYTWTTNPGSASCSKRWSVRAVMTHERGHTFGLAHVGEST
                     HGDLTMSPSINGPCQDSESTLGDGDVQGLAAMY"
BASE COUNT         1604 a         3495 c         3702 g         1451 t
ORIGIN      
        1 tcgagctccc ggtgaccgta agagcggtag tgtggctggt atctaccgtc acttctccgg
       61 cggatgtccc gctcagcgtg acgactccgt ctgtctcgcc tgtgctcggt tgcgcgcaac
      121 tcagaatcaa aggcagccgc gcagcagttc cggctcgccg atcgcgtgtt gcacgatccg
      181 ccacgccgcg ctcgcaccgt cgtcacccac cagggctcgc cccggccagc cgacccgcgc
      241 cagcacctcg cgcaggcgcg ccgcgttgcg tcggtgcacc gcctccatct ccgggtggta
      301 gccgtcgaac aggctgccgt cgcgcgccag tcgctcgcgc gtctcgtcgt cctcggcgat
      361 ccacctgatc agctcggcgc gcagatcctc gtccatgcac cgagcattgc cgcggtcgcg
      421 tccgcatgca acccgcccgc tcgcgtggac ctcgacgacc cagcggggcg gcaaggtcga
      481 cgcggcggcg cgtgcggcca cggtccacat accccgagct gcccgatcga cgccccgtcg
      541 cacgacttcg ccggcgtgtc gcggacgccg tgccgcgggc tgccgcgcgc cgccaaatcg
      601 atacaccgcg ccgtcattca tcgtgtccca cgaacgccat aatcaccgga gccgccgccc
      661 gtcagctcat tcacagctcg gccaggagga tcggcccgta ggggtcgctg ccgcccgcga
      721 gctcgccggt gcgccgcggg tcgcgacggt cgatctccgt gacgaggcgg tcgatcgtca
      781 gcggaccagg ggcaagggcg gcggtgaggc cggcgtccca ttcgagccgc ttgtgaaggc
      841 ctccggcgag cagcagcagc atgtcgccgg ggcggcgttc gaggtgcatc atcgccggcg
      901 ggccacgcac gtcgcgagac agcaccagcg tgggcaagtc ggcgaacagc cgccgaatgg
      961 gccccagcag ccgctgttcg gcgggcagcg cccgccatgc gatgtcgtgc tcgtcgggcg
     1021 cggcctccgt cggcgtgagc ggccgggcga gcggaccgcg gagcagcatc ctgcgggtgt
     1081 gctcctcgct ctgcgcggcg acccgcccgt cgcgcagcag gtacgccttg tcgttgccga
     1141 cccacgcgag cgtgatccgc cggggctcgc acagggcgag gacgacggtc aggatcggga
     1201 ggtcgggctc gtcgttctcg gcgaaggacg gatcggcggc gaaacactgc gaccccgcgg
     1261 cgaatgcccc ggggagggcc gccaccgggt ccgggccgcg cagcaggtcg aacatcaagg
     1321 acagcgccgg actacccagc gtgcgagcgc cgcagtccca gccggactcg ctgccggtgg
     1381 cgaggccgac cagcgtgccc cacggggtgt cctcgacgcg tgtgccgtac ggcgcgtccg
     1441 cccgcaggca cacccgggtg acgcgcagcg tggagccgac ctctggacga atggcggtct
     1501 gcatgaggct gcgcgtcacg tcatccgatc tccgggtcgg ctgtccccga ggacaggtca
     1561 ggggcaggcg ttgccggggt cgtgggggac gctgtcgtcg acgtagagca gcgtcttgct
     1621 gtcggccgag gtgaagccgg gggtgttctc gccctcggcg gcgaaggcga agaagcgctc
     1681 gccgaacatg ccgggctcga agttgtcggc ggcgagctcg gcggcgacgt cgaagacgag
     1741 ctgggcctgc tcgggggtgc cctgcaggca ggtcatgttc gggccgggga tgcactcgac
     1801 ggcggcgtcc atggcgatgc cgccgggctt ggcgctggtc gtcggggcgc cgttgccgtc
     1861 gagcagcgat tgatagatgc gcaggcttcc ggcccagcgg aagcggacga ggtcgtgggc
     1921 gccgatcggc acgttgtagt cgagctgcgc gccgtcgttg ccgttgtaga tctcgacgac
     1981 gcggcagtcg gcgccgcgcg acgggtcgat cgcgcaacac gaaccagcga caggcggcag
     2041 cggggtgttg gttgggaccg ggaagcgcag gtcgaactgc atgttcatgc cggtctcgtg
     2101 gccggtgcac ttgtaggcgc actcgtccga gatgtggacg atgccggggc ggcggtcggt
     2161 ggtgtcgacg aggtactcgc cgaggtccgg cgagccgttg gcgcagctgt agttcgggcc
     2221 gccgacgcag ttggtcttcg ggccgctgtg caggccgccg ggcacgagct cgtcctgcaa
     2281 gacgtcgtgg acctggacga tgttgtgctc gccggtccag cgaaacagga tcaggtcgcc
     2341 ctgcctggcg agcagcaccg ggtcgaccca gtcgtagtgc gcgacggtct ggaagttgtt
     2401 gacctcgaag acggtgcagc cgggacggtc ggcgtactgc gcatagcgac cttcgtacac
     2461 gagggggtcg gcgagcacgg tgcacgggcg cggctcgtag tggtcgttct cgaactcggg
     2521 caccgtgacg ggcacggaga cgacgccgcc ggccgggtcg tcctggtcga cgaagaagta
     2581 gatcccaggg cggtaaccgc aggggaagct gccggtgtgc cactcgaaga cgccgtcgac
     2641 ctgcttgtcg ccgctgcgga tctgacccgg ccagccggcg tcgccgtact tcgccgtcgg
     2701 cgggatctgg ccggcgaagg tggcgacctg tagcacgttg tgcaggccgg tccagtggaa
     2761 gcgcacggtc tggccggcga tcgtgtcgtg gccgacgttc tcggccgccg gccatgcgag
     2821 gtcgacgacg gtgccgggct cgttgatgac cccggcgcag gcgtcgatcg gtgtgccggt
     2881 ggtctcggtg gtcggctcgc tggtggtcgg ctcgccggtg gtcggggtcg gcgtatcggt
     2941 gccctcgacg ccggtgctct gcggggcgtc gccagtgctc ccggtgctcg tcgagctggt
     3001 gccggtctcg tcggtgcctg cgccggacgc tccacagccg gcgagcgcga gcaggacgag
     3061 tagacgttgt gaaccgagct cgacgaccat gtgccccgcc cccgctctta tgggtatcac
     3121 gctggacggc gcgccgcctc ctcgcaggac cgccccctgc gcacggtggc gcgaaagatc
     3181 gtcgcgcggc agcggccggt gacgatcggg cagtggcggt ctgcaccgcc cggactggcg
     3241 cggcccggtt gcttgcgcga ctggatcgtc aaccatggtc gcgcatgcag acccatcttc
     3301 gatccctcac gctgctcctc ggcctcgcgc tcgtcccggc ttgcgccgga acgaaggccg
     3361 atgaaacccg ccccgtggag gagccgagcc agcccgacgc gcagcccgcg ggcacgaaca
     3421 cccgcagctt gacgatgtcc ggtgtgtacc tcgacccgga gctcgttcgc ctgtgcggca
     3481 tcacgacgcc gacggccctc ttcgagttcg actcggctgc ggtcgagggc gccgacaaca
     3541 gcggcctgct cgccgtggcg acctgcgtca gcaccggacc gctcaagggc cgccagatcc
     3601 agctgatcgg ccacaccgac ccgcgcggca ccgatgacca caatacgcag ctcggcaagt
     3661 cccgcgccga cgcggtcatg cagtttctcg ccaacgaggg tggtgtgccc gccgaccgca
     3721 tgaccaccct gtcggagggc gagaaggggg ccgacccgac caacgccgac ggctggtctt
     3781 atgatcggcg cgtcgacatc cgtctggtgc cctgagccag gcgcgggcgg catcatttca
     3841 cgggctcggg gcgacatcca gcttcgcgct gccccgcaac tgcaccccgg cgacgatcgc
     3901 ggggtcggtg acggtcacgg cgacgacccg gacctcgtcg atcttgccgc gcagccggat
     3961 caattcggag atccggtagt cggcgatcgc ggcgttgatc tgatcgcggt agccgtcgag
     4021 ctgcccggtg aagtcgaaga ccagtcgctc gcgcacgcgc cggacgatcg cgtcgtggag
     4081 catccactcg gcgacatcga cgagcgcgac gttcgtgcgt gagtcgtagg cgaggtcggt
     4141 gatcgacagc tggttgcgcc ccgggtcgag cgtcggggtg ccgacgaggt agaccagtcc
     4201 gtgcatatcg agccacgccc ccgggaggtc ggcggagaac tcgacggcga cggcgacgtg
     4261 ttccccgctc gggtagacct cgaccgcacc gatcgtcagc gcgacggact ggccgacgac
     4321 gtcgagcggg acatcgaaga cctgaccctc gaggcgctcg tcgagctgct gattcgcggc
     4381 cgcgaaggtg agcgacacgg gcacgtcgag ggcgacgcct ggttcgccgg gcggcgccga
     4441 gcgggccggc agcggcgtcg gctcggcggc gggcggctcg gccccgacga cgacgcggaa
     4501 caccccgtgg atcgcggcgt cgagggacac ctggccgtcg tgggcccgga cctcgccgag
     4561 gtacatcgcg gtcggccgca gcaccagcca caggtcgccg cgggggccga cccgcagggt
     4621 ccggaacgcg gcggcccacg cctgtgtgat cagcgggcgc gtcgggatct gccggcgcag
     4681 ctgctcgtcg agcgtgcgcg tgacgccggc gaattgctcg tcgatgcggt cgccgacgag
     4741 cgcgctcagc ttgacgttca agggcccgac cggcacgacc gggtcgtcct cccagcgcag
     4801 cgtcgcctcg gtcgaggtcg ccaggttcca ctgcgcgtcg acaccgaggt cgatctcggc
     4861 gcgaaccacg aaggtggcgc gggtcttgat gtgctgacgt ttgccgcgcg acatgagacc
     4921 gacgaggccc gccggctgcc agtcggcctc gatgtcggct tcgatcggga cgcgcaggtg
     4981 gagcttgcct gcctccgcgc cggcggtgat gcggccgcca cggcgcacga cgaccccggc
     5041 cgtcacgccc tcgccggcgg cgatccccgg acgcgccagg aggtcatgcg ggagcttgtc
     5101 gttggcgatg ccctcgatca gcgcagcgtc gaccgcgatc gggacgctga cgctcgactg
     5161 actcgcggtc ggcggcgcgc ccggcaagct cgcgccgcgc ggcggcggct cgggctgcac
     5221 ccgatgcttg tagcgccacg cgaagtaccc ggcgccgccg agcaccagca ccagcacgag
     5281 cagcaggacc ccgcggagct tcgtcatggc tgcccataac atcatcagcc gcgggccggg
     5341 gtcgaaatac cgaccgaagg ttcacgcgag cggggcgggc tcgaccgcgg tcccgcggat
     5401 cgccgcgacg tcgcgggccg gtggcgcgcc gaactggcgc cggtactcgc gcatcgtctg
     5461 ctcaagggcg acgtgaagta ccggttcgtg atcgacatgg cctcgctgcg cgcctgacga
     5521 ccgctcagga tgacggtcgt ccgcacctgg aagggtgcca gcggctcagc gcgcggtcgg
     5581 cctcgagtga cgccgcgcga gggcgcccgc gatcgcgctt tgcagcgtgc tcgcggtctc
     5641 gatgcccgag aggtcgagcc cgaggtcgat ccacgtgcgg gcgacggtcg gctggatccc
     5701 cgtcagtacg gtgcgcaccc cgagcagcgc gagcgcccgc gtcgtcgcca gcagcagttt
     5761 ggcgacctcg gcgtcgatct gctgcaggcc ggtgacgtcg atcagcacga cctcgacgcc
     5821 gcgcccgtgc acgccgtcga gcgcggcgcg ggtcacctgc gacgcgcgct cgacgtcgat
     5881 ctgaccgatc aagggcatga cgaggacgcg gtcggagatc gggatcagcg gggtcgccag
     5941 gtcctggacc gcggcgcgca ggcgctgctc gatctgcttg acgtcggaga tgtcgcggat
     6001 caacgagccc cacccgacga tcgcgccgtc gacgtcgcgc agcgtgacga gcacttgata
     6061 aatcggcatg accgatccgt cttcaagcag caggtcgccc tcgccgctcc acgtgccgcc
     6121 agcgcgaagg atcgggcgga tctcccgctc gaggcgctcg gtgaacgccg gactgtgctt
     6181 caccgtgatc ttcgtggagc cctcgggatg gccccacgca tgcacgatcc gccgcgccga
     6241 ggcgttgcga tagaggaccc gcccctggac gtcgatgatg ccgacttggt cgggcgtgtt
     6301 ctcgagcatc gcctcgaaca gccgcagccg cgcgagtgcc tgctcgttgt ggcgcaccgt
     6361 ctccctgtgc gccgagatgt cgaccgcgct cgcgtaccag acccgctcgt caggcggcag
     6421 ggcgtcgtcg acggagcagc tccacatcaa ccacttgtag ctgccgtcct ggcagcggca
     6481 acggttctcg agctggaccg ggcgcccccg ggtcatccgc tcggcggcgg cgagggccgc
     6541 cgggcggtcg tcggggtgga tgagctcgat cggcgtgagg gcccgcagct cgtcgagcgt
     6601 ccagccgagc gtcttctccc acgccgggtt gagcgcgacg aagcgcccct gaggatcgat
     6661 cgtcgccatc atcgccgtcg agagcatgaa cagccgctcg aacgtcgagc tcatccgggc
     6721 cccctgcgat ccgccgcgcc agccatcacg gcgacgatgg ccgccggtcg ggctgcacgt
     6781 caacgcagat ttgctggtca tggtgcggcc ggatcatcga aacagcacgg acactggtcc
     6841 ggacgccccg gggggcggcg ccacgaatgc gtatgtaagc tgaatgtata tcgtgaatgt
     6901 cgacaatggc acggccgcga tgacgcgctc gcgctcgcgc ggggcgcctc gcgcgggcga
     6961 aaacgccgcg gccggtgcgc gacctcgaca gacggtgtca tgatagcgtc gtcgtgttgg
     7021 gggtggagat gtccgatcgc gagcgagtga agcgaacgaa cgtggccgat gtcgaggcga
     7081 aggaggtcgt gcacgcgacc gcgacgacgc cgctgcagct gctggcgtcg gccgacgccc
     7141 ggccgccgca cggggggctc ttgcagctgc aagggctgat cggcaaccgg gcggtcgctc
     7201 gcctgcgcgg tggttcgcgg gacgcggcgc tgagccacca ggtggccgcg gcgggcatcc
     7261 gcggcgtggg caggccgctg ccgcacctcg agcagatcca ggccgcgttc ggggggcacg
     7321 acgtccgcca ggtccgcgcc ttcaccggcg gggccgcggc gacgacgagc gaggcgctcg
     7381 gcgccgaggc ctacgcgacc cgcgagcgca tagcctttcg cagcgccggc ccgagcctgc
     7441 acacgaccgc gcacgaggcc gcgcatatca tccagcagcg ccgcgggatc cacctgccgg
     7501 gcgggttcgg ccgcgccggc gacagccacg agcgtcacgc cgacctcgtc gccgaccgcg
     7561 tcgtccgcgg tcaatccgcc gagtcgctgc tcggccgacc catcgggccc ggcagcttcg
     7621 cctcgccgac ggtccagcgg gcgccgctga gcgcgaggga tctcgggcgg ctctacgcgg
     7681 cggccgagat ggaccagaca aacttcaccc ggctggtaag ccactgggcg aaggagaccg
     7741 gcggcgccgc caaggtgcgt cacgaggtca aggatatggg ccgcctggcc cagaaggtcc
     7801 gggagaaggc gcgtgaggac ggcatcgagg aagacgtcgc gatcgggcgg atgtacgacg
     7861 tgcttggcgg gacgatcgtc tacaagaacc tggacgactt cttcgcgggt tacaacaagc
     7921 tcaaagatga gctggcaccc gcggccaggg cgaccatcgt ccgcctcaag aatcgcatca
     7981 cgcagaagaa caaccgcgac tttttgatca acatcaagct cgagtccggc ttcatcgtcg
     8041 agctgcagct acacctcgag gcgacgctcg ccggcaagca gggcgacccg gtgaaggtca
     8101 aggccgacag ggagagacag ggcggatttg gccgctatac cggccacgac gcctacgatt
     8161 acacgcgcat cctggagggc ttctttgggg cggtcgctcg gcagacagcc ggaaatcagc
     8221 gccagatcgt gctccccggc cgaaaaatca gcccggagaa ccgccgtcgg atggccacca
     8281 atcgcaacct cgcgcaagcc cagggcgacc aaagagagct cgccgcgatc aaggccgacc
     8341 tcaggaggcc gttcgtccag aaggacctca acaagtatta cgaggagctc accacgctct
     8401 ataaccagat ccaggacgag atcaccgcga acgcctggga ggaggtgctg tccaagggcc
     8461 gtgggcggct aaatgacatc gagggcattc actcgatcga caccctggcc gagcaagacc
     8521 tcggcgcgat cggcgagcac tggaaggcca acggcgatac ccgcgctctc atcaatacgc
     8581 ggtacagaaa gttcctgagc gaggccgacg ctcacgcgaa cgccgcggca gccggcccgg
     8641 ccagcatgct cgcgaccttc aggatgtact acaacatcct gaacgagaag cacgaggtga
     8701 agaagaaaaa gaaggccaat cagaaggtcc cggcctatca cgccaagatc gacacatacc
     8761 tcaagaccat ggcgagcgcg atcaagatcc tcgaggcgca agaggagcag cggctgcaac
     8821 tcgcgccgca gcagccgcag cagttacctc agcaacaggc tccggcgcaa ccacagtgac
     8881 gcgcagcgtc gtccccgggt gactcgacga tcgtgcggat ctgcgcttcg tcggtctcgc
     8941 cgtcctccag gttcacctgc gaatcgtcgc tggtcgccat cgtgctcgcc cgagcatcgc
     9001 ggcgcgccgc ggcgggcaag cccggcgcga tcgggccgcg tgcggtcggc gaaaaataat
     9061 tgcacccgcg tgagacaggc cagccgcgcc cgggaacaca ggaccgggcg gcgatgaggc
     9121 gcccgctcgc ggcgcgcggt gatgcggccg ctcgcagcct cgggagaaat caatgacgcg
     9181 gatgagccac atgaagatcg tcgacctgtc cctgttcgca aacctgttcg tggtcggctg
     9241 catcgacgac ggccaggccg cacccgatga tgtcgcggat gccgaggatg tcgaggagct
     9301 cgaggacatc gaggatgcgc ccgcgggcga gctcgacggt ctgcaggccc tcgcgccgga
     9361 gccgggcagc tcggtgtggt cggaggtgct gctcaccgac ggcaccacgc agcagttcat
     9421 gctgcacacg acgctcgcgg gcgaggtcgt gttcgaggag ctcagcgacg agcggttccc
     9481 gcaggcgccc gacctgacga ccctgccgga gcaggcggcg gcgctcgggg cctgctccga
     9541 cgacgcgtac atccttcagg gctggcgctg gaagtcggcg ctgcgctggt cgttccacgc
     9601 cggctcgacg ccggacgagc tgacggtgga cgccgcggag gcggcgatca cggcggcgac
     9661 cgcgagcatc acgtcggcgc gcaacagctg tgggctcgcg gacgaggtcg acgcgtcgca
     9721 cgagtacctc gggcgcaagc acaagggcgc gaacatcggc gccagcggca actgtctcac
     9781 gtccgacggc gacaacgtgg tctcgttcgg cgacctgccg cagggggtgc tggcgaccgc
     9841 gtgcgtgtgg ttccacgacg gcgtggcgac cgagggcgac gtgcagctca acaagcagga
     9901 ttacacgtgg acgacgaacc cggggtcggc gtcgtgttcg aagcgctgga gcgtccgggc
     9961 ggtgatgacc cacgagcgcg gccacacctt cggtctcgcg catgtgggcg agagcaccca
    10021 cggcgacctg acgatgagcc cgtcgatcaa cgggccgtgc caggacagcg agtcgaccct
    10081 cggggacggc gacgtacagg gccttgcggc gatgtattga gatttttgcg ggccccgcga
    10141 gacaagcggg cggggtccgg gaacttgggt agcgggcccg gcagcgccgg gcgaacaaat
    10201 gtcagccggc cgccggcatc gccgcgcacg acccttcggg gcggtgtcgc gg
//
LOCUS       DASWNJ010000219         1537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_604804, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000219 DASWNJ010000000
VERSION     DASWNJ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604804"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VGB85_04050"
     CDS             <1..793
                     /locus_tag="VGB85_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133221.1"
                     /translation="DNGFCTSQCRLNYCGDGHLFVGWELCDNGVANSDFYGSTCGEQC
                     TPAPRCGDHIVQAAEGEECDLGPDNGGLKGDEQGILCEATCRSKSLRAFVTSEAFGGD
                     LGGLDGADKKCRDAAMTAGLGEAYRFHAYLSTPDFSANDRFPGPMAEPLPYILLTGKK
                     LADSHAKLLYEGPLGEGLSVTELGESLYDTAVATNTSPSGKSYSPDQHCLAWTSADPL
                     LKARYGFTFPSDPGQLAGWASNGEWVSKSFVGCKDPILHLYCLEI"
     gene            875..>1537
                     /locus_tag="VGB85_04055"
     CDS             875..>1537
                     /locus_tag="VGB85_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0133222.1"
                     /translation="MLPFLTLISGCAIERLEAIFDEHGVTYNTTDVPDSDTAADTSTG
                     PGDTGSTSAADAGGEGASQEVSSEPETASTSTTGDPGTTGEETTGGPQPVCGNGVLEK
                     FGWVPEECDDGNLIPDDGCEDNCSADRTMFVSSKRYQAGDFKSLYLADALCAQAANDA
                     GLANWLKFRAWLSDSKTSARDRFKFSRGRIVLVNGLVVADSWIDLLAGKLQNPPEVTE
                     KGE"
BASE COUNT          291 a          471 c          541 g          234 t
ORIGIN      
        1 cgacaacggc ttttgtacga gccagtgccg gctcaattac tgcggcgacg gccacctgtt
       61 cgtcggctgg gagctgtgtg acaatggtgt ggcgaatagt gatttttacg gcagcacctg
      121 cggcgaacaa tgtacaccgg cgccgagatg cggtgaccac atcgtgcagg cggccgaggg
      181 cgaggagtgt gacctcgggc cggataatgg cggcctcaag ggcgacgagc aaggcatttt
      241 gtgcgaggcg acgtgccgca gcaagagcct gcgagcgttc gtcaccagcg aggcgttcgg
      301 cggggacctc gggggcctcg acggcgcgga caaaaagtgc cgcgacgcgg cgatgaccgc
      361 cggcctcggg gaggcgtacc gcttccacgc gtacctgagc acgcccgact tctccgccaa
      421 cgaccgcttc cccggtccga tggcggagcc gttgccgtat attttgctga ccgggaagaa
      481 gctcgccgac tcgcacgcga agctgctgta cgaagggccg ctcggggagg ggctgtcggt
      541 gacggagctc ggggagagct tgtatgacac ggcagtggct accaacacga gcccgagcgg
      601 caagagctac agcccggacc agcactgcct ggcgtggacc agcgcggacc cgctgctcaa
      661 ggcccgttac gggttcacct tcccgagcga ccctggccag cttgcgggct gggcgagcaa
      721 cggggagtgg gtcagcaaga gcttcgtcgg ttgcaaggat ccgattctcc acctctactg
      781 cctcgagatc tgaccacgcg tgccaccccg ctcggacgtc ccctggccct ccactcaccg
      841 atcccactac atgcaccgcc aagacgccag actcatgctc cccttcctca cgctgatttc
      901 aggctgcgcc atcgagcgtc tggaggcgat cttcgatgaa catggggtta cgtacaacac
      961 gacggatgta cccgacagcg atacagcggc tgacacatcc acgggcccgg gcgacacagg
     1021 ctcgacgagc gcggcggacg ccggtggcga gggggccagc caggaagtca gcagcgaacc
     1081 cgagacggcc tcgacgagca cgacgggcga cccgggaacg acgggcgagg agaccaccgg
     1141 gggcccgcag ccggtgtgtg gcaacggcgt gctcgagaag ttcggatggg tgccggagga
     1201 gtgtgacgac gggaacctga tcccggacga tggttgcgaa gacaactgct cggcggaccg
     1261 caccatgttc gtgtcgagca agcgctacca ggctggcgac ttcaagagcc tgtaccttgc
     1321 ggacgcattg tgcgcgcagg cggcgaacga tgccgggttg gcgaactggc tgaagtttcg
     1381 cgcgtggctc agcgatagca agacgagcgc gcgcgaccgg ttcaagttca gcaggggtcg
     1441 catcgtgctg gtcaacgggc tggtggtcgc ggactcctgg atcgacctgc tggcggggaa
     1501 gctgcagaat ccgcctgagg tcacggagaa gggggag
//
LOCUS       DASWNJ010000220         4260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_428188, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000220 DASWNJ010000000
VERSION     DASWNJ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428188"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2220
                     /locus_tag="VGB85_04060"
     CDS             <1..2220
                     /locus_tag="VGB85_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133223.1"
                     /translation="KVELMQDIHHQYYARLAPTMARMAADNPELSSERRRALDQFVKT
                     VLLAEVSPRLKQGGLTIERLVQLNSADVDGETFRGQVLVAETDLEALSNRLPDLQVIG
                     KGKIATVSYVLGRVSLGEVLGRARSKVDNSGQRFRVFWAALKAALKIGGAKGFEEGGP
                     NEGEWDLQWRKTRRRGQLKLGNVREMSNEDFDPPDGAFKVLIDYPWDEAGHTVDEDRL
                     RATNVRKKKGVLYTACWLPRHMSSAELGVLTELAAVRYLLSAVGQEELLETLGPQDRA
                     KVIGQAESRANNLTGQLEELLREVYVHHGEFFSLISDIDGSRPKETLHENLEHIAGLL
                     MDRRYSLHPHFLAEPKKADLEALLEWMVQAGETNVSVAYDETKGKVLRTLGQPLELVN
                     LGQTKASLRLDSRHIKDVLQGTDHDSVSWAPIAEQLRDTYGFQPLVIDLFLCFLCQRD
                     HRALQEIDGEPIEVKIGMSPTIRVRLQRGKLVSAAEWHRLRDLGDQLLAVKRPSANRS
                     LQAQDRFAAAAKEQGSKRRTVLQSLHKQLIDLGVEAGDRLKQLADANTRLAPLALSTT
                     DSHKVLGDLLTLWPDDPSDPLRGVVAQCEEIRNALAELNVNSRNNLQAGVNHPTLGVE
                     VNDHLGALDLRLRSAEAEQPLTKDWISTWNRKAQQLVQRLLEQPPPPPPPPPPPPPPP
                     PPPPPPPVTPMKVLLQRQLSPSDPDAISSFLADVRKALAGQGGASIRIALVREEDDA"
     gene            2217..>4260
                     /gene="pglZ"
                     /locus_tag="VGB85_04065"
     CDS             2217..>4260
                     /gene="pglZ"
                     /locus_tag="VGB85_04065"
                     /inference="COORDINATES: protein motif:HMM:NF033446.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-2 system phosphatase PglZ"
                     /protein_id="HEY0133224.1"
                     /translation="MSAAQLLLRRLLDQAGGAEETGYMLVIDGEGLEDLPEQMSTPKG
                     PYRVHRITTELGLRHLLWKAKGAPLIAVMPEDVARRIQKAPDLLRRARNQRIHALSIN
                     DVLEVVLGVRVVGADAPHMQQLALEHVNQLGVVMSHRTLPTVVDRRLLTEMLVDVSVG
                     EKVRTQTPAQLLAAWVQAPPLWPENVRLLVRDALLTMHGDDGRLLSWALLEPDHRLRE
                     LVIFGAVLTVEVQELPKTVWGPLWKAAAEAPIELDRRILRRVATRLAEDTLAILGDIA
                     APLLSDADRVARECLSPPQVETSHVLPLAFANRCHALAKQAAAGKPISGAEVAWLAAH
                     RAARMHRVSLAVLEAMARLSRFLDQPFTPRPDLLDQVRDYQRNGAFADLSILQLRRAL
                     ASSTDHHTDANKVLAAARERRNRENRQFADVLAAGYEGALHQAALTPLHRLWKRTVAP
                     VWEQEPGARIYLVVLDGCSYPVFLELLHALSQDNTFPIGVRPDLDGRVGGLPAIAPLP
                     TVTSHARGAIFLGELPQDPLVAETVFGDHDEAKTDRARLNQNAALGQRGRKLFLKGDL
                     ADGGQALLTALADPSLDVVAAVFNAVDDQIGSSNTGAAVKLSPEDITAFKPSLRGAIN
                     ARRKILVTADHGHSPYIDKSLRRSAGKTPRYLPLAKNAAVPAGFIEIDLAGLGGPPE"
BASE COUNT          705 a         1621 c         1354 g          580 t
ORIGIN      
        1 aaggtcgagc tgatgcagga catccaccac caatactatg cgcggctcgc cccgacgatg
       61 gcccggatgg cggcggacaa ccccgagctc agcagcgagc gccgccgggc gctcgaccaa
      121 ttcgtcaaga ccgtcctgct cgccgaggtc tcgccgcgcc tgaagcaggg cggcctcacc
      181 atcgagcgcc tcgtgcagct caactcggcc gacgtcgacg gcgagacctt ccgcggccag
      241 gtgctcgtcg ccgagacgga cctcgaggcc ctctccaacc gcctcccgga cctccaggtc
      301 atcggcaagg gcaagatcgc cacggtcagc tacgtgctcg ggcgcgtcag cctcggcgag
      361 gtcctcggcc gcgcccgctc gaaggtcgac aactcgggcc agcgcttccg ggtgttctgg
      421 gccgccctca aggccgccct caagatcggc ggcgcgaagg gcttcgagga gggcggcccc
      481 aacgagggcg agtgggacct gcagtggaga aagacccggc gccgcggtca gctcaagctc
      541 ggcaacgtgc gcgagatgtc gaacgaggac ttcgacccgc ccgacggcgc cttcaaggtc
      601 ctcatcgatt atccctggga cgaggccggc cacaccgtcg acgaggaccg cctccgcgcc
      661 accaacgttc gcaagaagaa gggcgtcctg tacaccgcct gctggctgcc gcgccacatg
      721 agctcggcgg agctcggcgt gctcaccgag ctggccgcgg tccgctacct tctctcggcg
      781 gtcgggcagg aggagttgct cgagaccctc gggccgcagg accgcgccaa ggtcatcggc
      841 caggccgaga gccgcgccaa caacctcacg ggccagctcg aggagctgct cagggaggtc
      901 tacgtccacc acggcgagtt cttctcgctg atctccgaca tcgacggcag ccgcccgaag
      961 gagaccctgc acgagaacct cgagcatatc gccggactgc tgatggaccg cagatattcg
     1021 ctgcacccgc acttcctggc cgagccgaag aaggccgacc tcgaggcgct gctcgagtgg
     1081 atggtgcagg ccggtgagac caacgtctcg gtcgcctacg acgagaccaa gggcaaggtc
     1141 ctgcgcacgc tcggccagcc gctcgagctc gtcaacctcg gccagaccaa ggcgtcgctg
     1201 cgcctcgact cgcgccatat caaagatgtc ctccagggca ccgaccacga ctccgtctcc
     1261 tgggccccga tcgccgagca gctgcgcgat acatacggct tccagccgct cgtcatcgac
     1321 ctcttcctgt gcttcctgtg ccagcgtgac caccgcgccc tgcaggagat cgacggcgaa
     1381 ccgatcgagg tcaagatcgg catgtcgccg acgatccgcg tgcggctcca gcgcgggaag
     1441 ctggtcagcg ccgcggaatg gcaccgactg cgcgacctcg gcgaccagct gctcgcggtc
     1501 aagcgcccgt ccgccaaccg ctccttgcag gcgcaggacc gcttcgccgc ggccgcgaag
     1561 gagcagggca gcaagcggcg cacggtcctc cagagcctcc acaaacagct catcgacctc
     1621 ggcgtcgagg ccggcgaccg cctcaagcag ctcgcggacg cgaacacccg cctcgcgccg
     1681 ctcgccctct cgaccacgga cagccacaag gtcctcggcg acctgctcac attgtggccc
     1741 gacgatccct cggatccgct gcggggcgtc gttgcgcaat gcgaggagat ccgcaacgcg
     1801 ctcgccgagc tcaacgtcaa ctctcgcaac aatctccagg ccggcgtgaa ccacccgacg
     1861 ctgggagtcg aggtcaacga ccacctcggc gcgctcgacc tccgcctccg ctccgcggaa
     1921 gccgagcagc ccctgacaaa agactggatc tccacctgga accgcaaggc gcagcagctc
     1981 gtccagcgcc tgctcgaaca gccgccgcca ccacccccgc caccaccgcc accacccccg
     2041 ccaccaccgc cgcctccgcc acctccggtc acgccgatga aggtcctgct gcagcgccag
     2101 ctcagcccgt ccgatcctga cgcgatctcg agcttcctcg ccgacgtacg aaaggcgctc
     2161 gcggggcagg gcggggcgag cattcgcatc gccctcgtcc gtgaggagga cgacgcgtga
     2221 gcgccgccca gctgctcctg cgccgtctgc tcgaccaggc cggcggcgcc gaggagaccg
     2281 gctatatgct cgtgatcgac ggcgagggtc tcgaggatct gccggagcag atgtcgacac
     2341 ccaaggggcc gtatcgtgtc caccggatca cgacggagct gggcctgcgc cacctcctgt
     2401 ggaaggccaa gggcgcgccg ctgatcgcgg tgatgccgga ggacgtcgcc cgcaggatcc
     2461 agaaggcccc cgacctcctt cgtcgcgccc gcaaccagcg gatccacgcg ctctccatca
     2521 atgacgtgct cgaggtggtc ctcggcgtgc gcgtcgtcgg cgccgacgcg ccccacatgc
     2581 agcagctcgc gctcgagcac gtcaaccagc tcggcgtggt gatgagccac cgcaccctgc
     2641 cgaccgtcgt tgatcggcgc ctgctcaccg agatgctggt cgacgtcagc gtcggcgaga
     2701 aggtccgcac ccagacgccg gcgcaactgc tcgccgcgtg ggtgcaggcg ccgccgctgt
     2761 ggccggagaa cgtccgcctg ctggtgcgcg acgccctcct cacgatgcac ggcgacgacg
     2821 gccgcctgct gtcctgggcg ctcctcgagc ccgaccaccg cctgcgcgag ctcgtgatct
     2881 tcggcgcggt gctgacggtc gaggtgcagg agctgccgaa gaccgtgtgg ggtccgctgt
     2941 ggaaggccgc cgccgaggcg ccgatcgagc tcgaccggcg gatcctccgg cgcgtggcca
     3001 cccgcctcgc cgaggacacc ctggcgatcc tcggcgacat cgcggccccg ctgctgagcg
     3061 acgccgatcg cgtcgcacgc gagtgcctgt cgcccccgca ggtcgagaca agccacgtgc
     3121 tcccgctggc gttcgcaaat cgctgccacg cgctcgccaa gcaggccgcc gcgggcaagc
     3181 cgatcagcgg ggccgaggtc gcgtggctgg ccgcccaccg cgcggcccgc atgcaccgcg
     3241 tcagcctcgc cgtgctcgag gcgatggcgc ggctgtctcg cttcctcgat cagcccttca
     3301 cccccaggcc cgatctgctc gaccaggtcc gcgactatca gcgcaacggg gcgttcgccg
     3361 atctgtcgat cctgcagctg cgccgcgccc tcgccagcag caccgatcat cacaccgacg
     3421 cgaacaaggt cctggccgcc gctcgtgagc ggcgaaaccg cgagaaccgg caattcgccg
     3481 atgtgctcgc cgccggctat gaaggcgcgc tgcaccaggc ggccctcacc ccgctgcatc
     3541 gcctgtggaa gcgcaccgtc gccccggtat gggagcagga gcccggcgcg cggatctacc
     3601 tggtcgtgct cgacggatgc agctatcccg tgttcctcga gctgctgcac gcgctatctc
     3661 aggacaacac cttcccgatc ggcgtgcgcc ccgatctcga cggacgcgtg ggcggcttac
     3721 cggccatcgc gcccctaccc actgtgacca gccacgcgcg cggggcgatc tttctcggcg
     3781 agctcccgca agatccgctg gtcgccgaga ccgtattcgg ggaccacgac gaggcgaaga
     3841 ccgacagggc ccgcctcaac cagaacgccg ccctcggcca gcgcggccgc aagctgttcc
     3901 tgaagggcga cctcgcggac ggcggccagg ccctgctcac cgcgctcgcc gacccgtcgc
     3961 tcgatgtcgt cgccgcggtc ttcaacgccg ttgacgacca gatcgggtcg tcgaacaccg
     4021 gagcggccgt caagctcagc cccgaggata tcaccgcatt caagccgagc ctgcgtggcg
     4081 ccatcaatgc gcggcgcaag atcctggtca ccgcggatca cggacacagc ccctatatcg
     4141 ataagagcct gcgccggagc gccggcaaga ccccgcgcta cctgccactc gccaagaacg
     4201 ctgcggtccc ggccggcttc atcgagatcg acctcgcagg gctcggggga ccaccggagc
//
LOCUS       DASWNJ010000221         1754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_215459, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000221 DASWNJ010000000
VERSION     DASWNJ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215459"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="VGB85_04070"
     CDS             <1..1131
                     /locus_tag="VGB85_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0133225.1"
                     /translation="VHINAFNFPAWGMLGKAAVALLAGMPVLSKPATSTAALAHRIVE
                     IVAEAKILPEGALQLLMGPAGDLLDHLGPQDVIAFTGGADTAAKLRVHASVLRHGTRL
                     NVEADSLNAVVLGPDLAEGSQLWDLALRDAVIELTQKTGQKCTATRRILVPSERLADF
                     RDALAERLGELAGKTGDPLSKAVKMGPLSTGQQLTDARKGLAKLQQHARIVRGDPERR
                     DFLDVPAGKGFFLEPILLEANTGSVLDPAASFHEVEVFGPVATLLPYDGTIAQAAKAV
                     ALGGGSLVSTLYSDDREFAAKAVAEIGPSLGRLVLNDEKSAAGAFSPGCVFPQANHGG
                     PGRAGGGSELGGRLGMELYMQRIAVQGGASQLARLLGGKPAA"
     gene            1298..1426
                     /locus_tag="VGB85_04075"
     CDS             1298..1426
                     /locus_tag="VGB85_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133226.1"
                     /translation="MSLVVQFMQFWPVPMPNPSVPVHTALVLHGIMLAMMSSPASV"
     gene            1426..>1754
                     /locus_tag="VGB85_04080"
     CDS             1426..>1754
                     /locus_tag="VGB85_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133227.1"
                     /translation="MQRLVEVVAARDVIGILEHLRERRMRDAVLERRVRDAVDRVAAG
                     SVVAQPGDELRAPGRLGHARALPREIRTQLVGLLRGREVRRAEGQARHEHEQRVALAV
                     VVAAVVD"
BASE COUNT          248 a          674 c          584 g          248 t
ORIGIN      
        1 gtgcacatca acgccttcaa cttcccggcc tggggcatgc tcggcaaggc cgccgtcgcc
       61 ctgctcgccg gcatgcccgt gctctcgaag ccggcgacga gcaccgccgc gctcgcgcac
      121 cgcatcgtcg agatcgtcgc cgaggccaag atcctgcccg agggcgcgtt gcagctgctg
      181 atgggcccgg ccggcgacct gctcgatcac ctcggcccgc aagatgtcat cgccttcacc
      241 ggcggcgccg acacggccgc gaagctgcgc gtgcacgcaa gcgtgctgcg ccacggcacc
      301 cgcctcaacg tcgaggccga cagcctcaac gccgtcgtgc tcggcccgga cctcgccgag
      361 ggcagccagc tctgggacct cgcgctgcgc gacgccgtga tcgagctgac ccagaagacc
      421 ggccagaagt gcaccgccac gcgccggatc ctggtcccga gcgagcgcct cgccgacttc
      481 cgcgacgccc tcgccgagcg cctcggcgag ctcgccggca agaccggcga cccgctgagc
      541 aaggcggtca agatgggccc gctgtcgacc ggccagcagc tgaccgacgc gcgcaagggc
      601 ctggcgaagc tgcagcagca cgcccgcatc gtccgcggcg accccgagcg ccgcgacttc
      661 ctcgacgtgc ccgccggcaa gggcttcttc ctcgagccga tcctgctcga ggccaacacc
      721 ggcagcgtgc tcgaccccgc cgccagcttc cacgaggtcg aggtgttcgg cccggtcgcc
      781 accctcctgc cctacgacgg cacgatcgcc caggccgcca aggccgtcgc gctcggcggt
      841 ggcagcctcg tcagcaccct ctacagcgac gaccgcgaat tcgccgcgaa ggccgtcgcc
      901 gagatcgggc cctcgctcgg tcgcctcgtg ctcaacgacg agaagtccgc cgccggcgcg
      961 tttagccccg gctgcgtgtt cccgcaggcc aaccacggcg gccccggccg cgccggcggc
     1021 ggctccgagc tcggcggtcg cctcggcatg gagctgtaca tgcagcggat cgccgtgcag
     1081 ggcggcgcca gccagctcgc ccgcctgctc ggcggcaaac cggcggcctg aagcgttgcg
     1141 cggacgcgct acacgtcgtg cgcccggccg ccgggcgccg ccgccacacc cggcctcgaa
     1201 gcagtagatc ggcagcgccg cgacgcagaa gcactgggta tcgatcgtcc aggcgtcgtc
     1261 gacggtcccg gtccgcccgg tcgtcgcctg cacgcccatg tcgttggtcg tccagttcat
     1321 gcagttctgg cccgtgccca tgccgaaccc gtccgtgccg gtccacaccg cgttggtgct
     1381 gcatggcatc atgttggcga tgatgtcgtc gccggcctcg gtgtagtgca gcgactcgtc
     1441 gaggttgttg ccgcccggga tgtcatcggt attcttgaac acctgcgcga gcgacggatg
     1501 cgtgacgcgg tactcgagcg gcgagtacgg gatgcggtcg atcgcgtcgc cgccgggtcc
     1561 gtcgtagctc agccaggcga cgaacttcga gctcctggcc gcctggggca cgcccgcgcc
     1621 cttccccgcg agatccgtac acagcttgtc ggccttctcc ggggtcgaga agtccggcgg
     1681 gccgagggcc aggctcgaca cgaacacgaa cagcgcgtcg cgctcgcagt cgttgttgca
     1741 gccgtcgtcg acga
//
LOCUS       DASWNJ010000222         6211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_391161, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000222 DASWNJ010000000
VERSION     DASWNJ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391161"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1482
                     /locus_tag="VGB85_04085"
     CDS             <1..1482
                     /locus_tag="VGB85_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HEY0133228.1"
                     /translation="DLSRGSVELTMAPFKLGKIPEVLARLPVVDSENATVGGDLKIAM
                     ADGKAEVEGDLQLAGLNIDHPMLARQPVLGLGFAFNFTGEVDPAQRRVVIEAATLRRG
                     GIEVDLDGELVHPEDPAARKYRLHVAVPKVACQEVIDAIPAELAPSLVGFQLKGDFEL
                     ELSADIDFADLEKLTLTGRVERDKCKAVKVPHLVSADRLGGAFVHRVTMRDGAQRTVD
                     LREGSPTYTALDQISPYMIAAVMTTEDGGFWRHRGFITSQFPAALRRNLEAGKIRLGA
                     STITMQMVKNVLLSHERTLSRKLQEMILTWHVEQTLSKERIMEVYLNVIEFGPGVYGI
                     TRAASHYFGKVPAELTPPEAAYLALMLPSPVRRHVHYCEGGLSPAFQAKLRRLLSIMQ
                     SRGRLDPETYELWKEGTITFNPAELDSKRACLGEIQRLLAASEQQRSISGLLDDSGYD
                     DEALPLAADEVEGEPAEGKRRKPDAPPPKSGEAEDAFLQAEER"
     gene            1479..2954
                     /locus_tag="VGB85_04090"
     CDS             1479..2954
                     /locus_tag="VGB85_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133229.1"
                     /translation="MKRTRLALAILLSFGTAEARAQEVLIEADASPHPPDTRTRKVHG
                     DIRFGAGFGFIVEDPIASLEPSFGLDFRDIAPVELRIGAPIRLRMYDRDPEQGPVIRN
                     TDWDEVGDYLAILQQLSYTGDYVFAEQGRAMVDLRVGGLGRVHVGHGSIVHGYANSLD
                     LDRRRTGVDLDARVEGALLEQPAGAELAFIVGDLAGQQIFGTRLGADWAGAGIGFSVF
                     GDPFAPRTLVPESTGSAALQIDSQNSLTTTGTRGATALGLDFSYRWTDRWRYLVVPYI
                     DLNLMPGLGKGMHIGVDTEFTLGRRRAVRLGVVGELTVGDAGYDPTYFDVFYMSQRMQ
                     GQFLAYPNDLPGGFASVAAPKYGFVQQNDLHGVGGYGALRFSHEEGAFVETGYRYRPG
                     PLGHTWETRVGVDLEVVQLSVLAAHKGPFGFDIVDPAGTVARLDLNVPVNPYLDVTAQ
                     AGWQPIVRRAPGEPATARQPSGGFVGGGGLILLGIAGRFGW"
     gene            2960..3733
                     /locus_tag="VGB85_04095"
     CDS             2960..3733
                     /locus_tag="VGB85_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY0133230.1"
                     /translation="MPGTIGQVPKDRPQFDGSRGLMDAPELLAAITRVGPDAVTATLA
                     PRLTPARKARIEAVLRGRVGSIQVAVEDPQDPHNAAAVVRTAEALGAGAVHMIGGSPR
                     ALQHRKVTRGTFYWADTHEHADIEGLAAVLPPGMRIAGACVDGPTMSLADVPVDRPLC
                     LLFGSEQSGLSAAARARCDLQFRVPMFGMAESLNLSVAAGIVLHALLERRRAHLGAPG
                     DLDEQELAVRRARWYAKAVDPRLARHALGLGETTKEHES"
     gene            3730..>6211
                     /locus_tag="VGB85_04100"
     CDS             3730..>6211
                     /locus_tag="VGB85_04100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEY0133231.1"
                     /translation="MTRRGRKIIGWTVGTLLTGAVLGVGSIAGVFWYYGRDVAHVDVA
                     AIRDYRPPQVTRIVARDGTVLGEIFSQRRTLIRFDEIPTHVVNAFLAAEDADFYNHEG
                     MDYFGMARAALTNLEAGRARQGASTITQQVVKNFLLSPERSFERKIQELILARRLEQA
                     LSKHEILELYLNEIFFGHGRYGIEEASKFYFGKSVREIDIGQAAVLAALPKAPGKSTP
                     LKEPEKAKARQVYVLEQMVANGFAQPKDVEAAIAGPMALNPSPESGPGRADAQEFVDA
                     ARELLLAKYGAERLDRLGATVTTTVDLDVQSKARVALFEGLVGLDHRQGYGHKIKPAK
                     LKEQAKVLDKGKVPLAIAGVVPVVIQARTPAVPADMFAAKAGDSDLLVHVPEGTRYDD
                     PKLTHDEQFPAGGVTMVTITALAGTPEAKAAGAPEGFAMGTIASGPQAAVVVAEAKTG
                     EVLAMIGGDHYRRGEFNRVLAAKRQPGSSFKPFIYGAALASEKFSAATLVSDSPEIYE
                     KWRPTNFEADVYRGDIRVRVALTFSVNTIAIKLLDAVGFEAAHAFARAAGIEAPLADN
                     LALALGVSEMTPRDLLAGYLTLARGGSYLEPTFIRSIDVPGEGTWTPERVPEQRLRDD
                     VVFVLTSMMTSVVQEGTGTGAKALKRPTAGKTGTSAEHRDAWFAGFTPDHVAVAWVGF
                     DTPKKLGKSETGGRAALPIWLGTMKAASADTPVHGFVPPASVQVRTIDKRTGLLAPLE
                     VPQPDGSMGPPKPEDVMEEYFVAGTEPVDVAEPAALPAGDALLDLYGDGDERIPSDQA
                     PAALDEATDPIPPPTTSPSSPPAAPPATP"
BASE COUNT          947 a         2315 c         2098 g          851 t
ORIGIN      
        1 gacctctcgc gcggcagcgt cgagctgacg atggccccct tcaagctcgg caagatcccc
       61 gaggtgctgg cccgcttgcc ggtcgtcgac tccgagaacg cgaccgtcgg cggtgacctc
      121 aagatcgcga tggccgacgg caaggccgag gtcgagggtg acctgcagct cgccggcctc
      181 aacatcgacc acccgatgct cgctcgccag ccagtgctcg gcctcggctt cgccttcaac
      241 ttcacgggcg aggtcgaccc ggcgcagcgg cgcgtcgtga tcgaggcggc gaccctgcga
      301 cgcggcggca tcgaggtcga cctcgacggt gagctcgtgc acccggagga cccggccgcc
      361 cgcaagtatc gcctgcacgt ggcggtgccg aaggtcgcct gccaggaggt gatcgatgcg
      421 atccccgccg agctcgcgcc gagcctcgtc ggcttccagc tcaaggggga cttcgagctc
      481 gagctcagcg ccgacatcga cttcgccgac ctcgagaagc tgacgctgac cggccgcgtc
      541 gagcgcgaca agtgcaaggc ggtcaaggtg ccgcacctgg tctcggcgga ccgcctcggc
      601 ggggccttcg tgcaccgcgt gacgatgcgc gacggtgccc agcggaccgt cgatctacgg
      661 gaaggctcac cgacctacac cgcgctcgac cagatctcgc cgtacatgat cgcggcggtg
      721 atgacgaccg aggacggcgg cttctggcgc caccgcggct tcatcacctc gcagttcccg
      781 gcggccctgc ggcgcaacct cgaggccggc aagatccgcc tcggggcctc gacgatcacc
      841 atgcagatgg tcaaaaacgt gctgctgtcg cacgagcgca cgctctcgcg aaaactgcag
      901 gagatgatcc tgacctggca cgtcgagcag acgctcagca aggagcgcat catggaggtc
      961 tacctcaacg tgatcgagtt cggccccggg gtgtatggca tcacccgcgc tgcctcgcat
     1021 tacttcggca aggttcccgc cgagctgacg ccgccggagg ccgcctacct cgcgctgatg
     1081 ttgccgagcc ccgtgcgccg ccacgtccac tactgcgagg gcggcctgtc gccggcgttc
     1141 caggccaagc tcaggcgcct gctctcgatc atgcagagcc gcggccggct cgaccccgag
     1201 acctacgagc tgtggaagga gggcacgatc accttcaacc ccgccgagct cgactccaag
     1261 cgcgcctgcc tcggcgagat ccagcgcctg ctggccgcct cggagcagca gcggtcgatc
     1321 tccggcctgc tcgacgacag cggctacgac gacgaggcgc tgccgctggc cgccgacgaa
     1381 gtcgagggtg agccggccga gggcaagcgc cgcaagccgg acgcgccgcc gccgaagtca
     1441 ggcgaggccg aggacgcgtt cttgcaggcg gaggagcggt gaagcgcacg cgcctcgcgc
     1501 tcgcgatcct cctgtccttt gggacagccg aggcgcgcgc gcaggaggtg ctgatcgagg
     1561 ccgacgcgag cccacacccg cccgacacgc gcacgcgcaa ggtccacggt gacattcgct
     1621 tcggggccgg cttcggcttc atcgtcgagg atccgatcgc cagcctcgag ccctcgttcg
     1681 ggctcgactt tcgcgacatt gcgccggtcg agctgcggat cggcgcgccg atccggctgc
     1741 gcatgtacga ccgcgacccc gagcagggcc cggtgatccg caacaccgac tgggacgagg
     1801 tcggcgacta cctggcgatc ctgcaacagc tctcgtacac cggcgactac gtgttcgccg
     1861 agcaggggcg ggcgatggtc gacctgcgcg tcggcggcct cgggcgcgtg cacgtcggcc
     1921 acggcagcat cgtgcacggc tacgccaaca gtctcgacct cgaccgccgg cgcaccggcg
     1981 tcgacctcga cgcgcgggtg gagggcgcgc tgctcgagca acctgcgggc gccgagctgg
     2041 ccttcatcgt cggcgacctc gctggccagc agatcttcgg cacgcggctc ggcgccgact
     2101 gggccggcgc gggcatcggc ttctcggtgt tcggcgaccc cttcgcgcca cgcaccctcg
     2161 tgccggagtc gactggcagc gccgccctgc agatcgacag ccagaacagc ctcaccacca
     2221 cgggcacgcg cggcgcgacc gcgctcggcc tcgacttcag ctaccgctgg accgaccgct
     2281 ggcgctacct cgtcgtcccc tacatcgacc tcaacctgat gcccgggctc ggcaagggca
     2341 tgcacatcgg cgtcgacacc gagtttacgc tcggccgccg tcgcgcggtg cggctcggcg
     2401 tcgtcggtga gctgaccgtc ggtgatgccg gctacgaccc gacctacttc gacgtgttct
     2461 acatgtcgca gcgcatgcag ggccagttcc tcgcctaccc gaacgacctc ccgggaggct
     2521 ttgccagcgt ggctgcgccc aagtacggct ttgtgcagca aaacgacctg cacggcgtcg
     2581 gcggttatgg tgcgctgcgc ttctcgcacg aggagggcgc gttcgtggag accggctatc
     2641 gctaccgccc cggcccgctc gggcacacct gggagacgcg cgtcggcgtc gacctcgagg
     2701 tcgtgcagct gtccgtgctc gcggcgcaca agggtccgtt cggcttcgac atcgtcgacc
     2761 cggccggcac cgtcgcgcgc ctcgacctca acgtgccggt caacccctac ctcgatgtca
     2821 ccgcccaggc cggctggcag ccgatcgtcc gccgcgcgcc cggcgagccc gccaccgcgc
     2881 gacaaccatc gggcggcttc gtcggcggcg gcggcctgat cctcctcggc atcgccggac
     2941 gcttcggctg gtgagctcca tgccaggtac gatcggacag gtcccaaagg acaggccgca
     3001 attcgacggc tcacgcggcc tgatggacgc cccggagctg ctcgccgcga tcacgcgcgt
     3061 cggccccgac gccgtcaccg ccacgctcgc gccgcgactg accccggccc gcaaggcgag
     3121 gatcgaggcc gtgctgcgcg ggcgcgtcgg atcgatccag gtcgccgtcg aggacccgca
     3181 ggacccgcac aacgccgccg cggtggtgcg caccgccgag gcgctcggcg ccggcgcggt
     3241 gcacatgatc ggcggctcgc cgcgcgcctt gcagcaccgc aaggtcaccc gcggcacgtt
     3301 ctactgggcc gacacgcacg agcacgccga catcgagggc ctggccgccg tgctgccgcc
     3361 gggaatgcgg atcgcaggcg cgtgcgtcga tggtccgacc atgagcctcg ccgacgtgcc
     3421 ggtcgatcgc ccgctgtgcc tgctgttcgg cagcgaacaa agcggcctga gcgccgcggc
     3481 acgggcccgc tgcgacctgc aatttcgcgt cccgatgttc ggcatggccg agagcctcaa
     3541 cctctcggtc gccgcgggga tcgtcttgca cgcgctgctg gagcgccgcc gcgctcacct
     3601 cggcgcgccc ggggacctcg acgaacagga actggccgtg cgacgcgcgc gctggtacgc
     3661 gaaggcggtc gacccgcggc tggcccggca cgcgctgggc ctcggcgaga cgacgaagga
     3721 gcacgagtca tgacgagacg cggacgcaag atcattggct ggaccgtcgg caccctgttg
     3781 acgggcgccg tcctcggcgt cggcagcatc gccggcgtgt tctggtatta cggccgcgac
     3841 gtcgcccacg tcgatgtcgc cgcgatccgc gactaccggc ccccgcaggt gacccgcatc
     3901 gtcgcccgcg acggcaccgt gctcggcgag atcttcagcc agcggcgcac gctgatccgc
     3961 ttcgacgaga tcccgaccca cgtggtcaac gccttcctcg ccgccgagga cgccgacttc
     4021 tacaaccacg agggcatgga ctacttcggc atggcccgcg ccgcgctgac caacctcgag
     4081 gccggccgcg cccgccaggg cgcctcgacg atcacccagc aggtcgtgaa gaacttccta
     4141 ctgtcgcccg agcgcagctt cgagcgcaag atccaggagc tgatcctcgc gcgtcgactc
     4201 gagcaggcgc tgtcgaagca cgagatcctc gagctctacc tcaacgaaat cttcttcggc
     4261 cacggccgct acgggatcga ggaggcgagc aagttctact tcggcaagag cgtccgcgag
     4321 atcgacatcg gccaggccgc cgtgctcgcc gcgctgccca aggcgcccgg caagtcgacc
     4381 ccgctcaagg agcccgagaa ggccaaggcg cgccaggtct acgtgctcga gcagatggtc
     4441 gccaacggct tcgcccagcc caaggacgtc gaggccgcga tcgccgggcc gatggcgctc
     4501 aaccccagcc cggagtccgg cccgggccgc gcggacgcgc aggagttcgt cgacgccgcg
     4561 cgcgagctcc tgctcgccaa gtacggcgcc gagcgcctcg atcgcctcgg cgcgacggtg
     4621 acgacgaccg tcgacctcga cgtccagagc aaggcccgcg tcgccctgtt cgagggcctc
     4681 gtcggcctcg atcaccgtca gggttacggc cacaagatca agccggccaa gctgaaggag
     4741 caggccaagg tgctcgacaa gggcaaggtg ccgctggcga tcgccggggt cgtgccggtc
     4801 gtgatccagg cgcgtacacc ggctgtccct gcggacatgt tcgcggccaa ggccggcgac
     4861 agcgacctgc tcgtgcacgt gccggagggc acccgctacg acgatccgaa gctgacccac
     4921 gacgagcagt tcccggccgg cggcgtgacg atggtgacga tcaccgcgct cgccggcacg
     4981 cccgaggcca aggccgccgg cgcgcccgag ggcttcgcca tgggcacgat cgcctcgggc
     5041 ccgcaggcgg cggtcgtcgt cgccgaggcg aagaccggcg aggtcctggc gatgatcggc
     5101 ggcgatcatt accgccgcgg cgagttcaac cgcgtgctcg cggccaagcg tcagccgggc
     5161 agctcgttca agccgttcat ctacggcgcc gcgctggcgt ccgagaagtt cagcgcggcg
     5221 acgctcgtca gcgactctcc cgagatctac gagaagtggc gcccgaccaa cttcgaggcc
     5281 gacgtgtacc gcggcgacat ccgcgtgcgc gtcgcgctga ccttctcggt caacacgatc
     5341 gcgatcaagc tgctcgacgc ggtcggcttc gaggcggccc acgcgttcgc ccgcgccgcg
     5401 gggatcgagg ccccgctcgc cgacaacctc gcgctcgcgc tcggcgtcag cgagatgacc
     5461 ccgcgcgacc tgctcgccgg ctacctgacg ctcgcgcgcg gcggctccta cctcgagccg
     5521 acctttatcc gttcgatcga cgtgcccggc gagggcacct ggacccccga gcgcgtgccc
     5581 gagcagcgcc tgcgcgacga cgtcgtgttc gtgctgacct cgatgatgac cagcgtcgtc
     5641 caggagggca ccggcaccgg cgccaaggcg ctcaagcggc cgaccgcggg caagaccggt
     5701 acctcggccg agcaccgcga cgcgtggttc gccggcttca cgcccgacca cgtcgccgtc
     5761 gcgtgggtcg gcttcgacac gccgaagaag ctcggcaaga gcgagaccgg cggccgcgcg
     5821 gccttgccga tctggctcgg cacgatgaag gccgcgagcg ccgacacccc tgtgcacggc
     5881 tttgtcccgc cggcatcggt gcaggtgcgc acgatcgaca agcgcacggg gctgctcgcg
     5941 ccactcgagg tgccgcagcc cgacggcagc atggggccgc ccaagcccga ggacgtgatg
     6001 gaggagtact tcgtcgccgg caccgagccg gtcgacgtcg ccgagccggc cgcgttgccc
     6061 gcgggtgatg cgctgctcga cctctacggt gacggcgacg agcgcatccc ctccgaccag
     6121 gctccggccg ccctcgatga ggcgaccgac ccgatcccgc cgccgaccac ttcgcccagc
     6181 agcccgcccg cggccccgcc cgcgacgccc g
//
LOCUS       DASWNJ010000223         1846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_604860, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000223 DASWNJ010000000
VERSION     DASWNJ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604860"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="VGB85_04105"
     CDS             <1..1006
                     /locus_tag="VGB85_04105"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HEY0133232.1"
                     /translation="LALRVMLGAGLIKLRGDACWTDLTCLEAHFETQPLPNPVSWYFH
                     HLPPWLLHAGVVFNHIAELALPLLAFGPRRLRILAGLGMIAFQVNLIVSGNLSFLNWL
                     TIVPCIACLDDRLLARVLPRRLSRDLVDAPATRRSHLTACVYAALVAWLSVPVVANML
                     GRGQAMNASFDRLHLVNTYGAFGSVSTVRLELEIEGTDATEPDERAVWRPYAFYCKPG
                     DPARRPCWAAPYHHRLDWLMWFAALDVAANGGLARETWLLALLDKLLAADPQVLSLMA
                     PPPNFAGGAPTFVRVEVYRYRFTAAGERAWWQRERLGSLIRPVSRDDPELRALLDDGA
                     "
     gene            1054..>1846
                     /locus_tag="VGB85_04110"
     CDS             1054..>1846
                     /locus_tag="VGB85_04110"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0133233.1"
                     /translation="MPRESWHTARLFSRRHAAELTVTHDGVTPRDDRGGAPNLTTPPA
                     PDDPGSAAPNPEGPPLQTRLGRFMLLQLLGQGGMGVVYTAWDELLDRRVALKLLRTGH
                     DGDAQARILHEAKGLARLSHPNVVQIYEVSASGGRLFIAMEYIAGNTLRAWTDEQRRA
                     GADPRVLLDMYIQAGRGLAAAHAQGLVHRDFKPDNVLVGEDGRARVVDFGLVTIRHAA
                     PDVHAPGGAPPAIDARLTADGAVHGTPAYMPPEQFAGLAADARSDQ"
BASE COUNT          238 a          718 c          614 g          276 t
ORIGIN      
        1 gctcgccctg cgcgtgatgc tcggcgccgg tttgatcaaa ctgcgcggcg acgcctgctg
       61 gaccgacctc acctgcctcg aggcgcactt cgagacccag ccgctgccca acccggtcag
      121 ctggtacttt catcacctgc cgccgtggct gctgcacgcc ggtgtcgtct tcaatcacat
      181 cgccgagctg gcgctcccgc tgctcgcgtt cggcccgcgc cgcctgcgga tcctcgccgg
      241 gctcggcatg atcgcgtttc aggtcaacct gatcgtcagc ggcaacctct cgttcctcaa
      301 ctggctgacg atcgtgccgt gcatcgcctg cctcgacgac cgcctgctcg cgcgggtact
      361 gccgaggcgg ctgtcccgtg atctcgtcga tgcgcccgcg acgcggaggt cgcatctcac
      421 cgcctgcgtg tacgcggcgc tcgtcgcgtg gctcagcgtc ccggtcgtcg ccaacatgct
      481 cgggcgcggc caggcgatga acgcctcctt cgatcgcctg cacctcgtca acacctacgg
      541 cgccttcggc agcgtcagca ccgtgcgcct cgagctcgag atcgagggca ccgacgccac
      601 ggagcccgac gagcgcgccg tgtggcgccc ctacgcgttt tattgcaagc ccggcgaccc
      661 ggcgcgccgg ccgtgctggg cggcccccta tcatcatcgc ctcgactggc tgatgtggtt
      721 cgccgcgctc gacgtcgccg ccaacggcgg gctcgcgcgc gagacctggc tgctggccct
      781 gctcgacaag ctgctcgccg ccgacccgca ggtgctctct ctgatggcac caccgccgaa
      841 cttcgccggc ggcgccccga ccttcgtgcg cgtcgaggtc tatcgctatc gcttcaccgc
      901 ggcgggcgag cgcgcgtggt ggcaacgcga gcgcctcggc tcgctgatcc gcccggtgtc
      961 ccgcgacgac ccggagctgc gcgcgctgct cgacgacggc gcgtgagcag cggcgacggg
     1021 cgagacggcg cagtggcaaa gcctgcgcag ggggtgcccc gcgaaagctg gcatactgcg
     1081 cgcctgttct cccgacgaca cgcggccgag ctcacggtca cacacgacgg cgtcacgccg
     1141 cgggacgatc gcggcggcgc gccgaacttg acgacgccgc ctgcccccga cgatccgggc
     1201 agcgccgcgc cgaaccccga gggtcccccg ttacagacgc ggctcggtcg cttcatgctg
     1261 ctgcagctgc tcggtcaggg cggcatgggc gtcgtctaca ccgcgtggga cgagctgctc
     1321 gaccggcggg tcgcgctcaa gctgctgcgc accggccacg acggcgacgc gcaggcgcgg
     1381 atcctccacg aggccaaggg cctggcccgc ctctcgcacc ccaacgtcgt ccagatctac
     1441 gaggtcagcg cctcgggcgg gcggctgttc atcgcgatgg agtacatcgc gggcaatacc
     1501 ctgcgcgcct ggaccgacga gcagcgccgc gccggcgccg acccacgtgt ccttctggac
     1561 atgtacattc aggcaggtcg tggcctcgcc gccgcgcacg cgcagggcct ggtccatcgc
     1621 gacttcaagc ctgacaacgt gctcgtcggc gaggacgggc gcgcccgcgt cgtcgacttc
     1681 gggctcgtca cgatccgcca cgccgcgccg gacgtgcacg cgccgggcgg cgccccgccg
     1741 gcgatcgacg cgcgcctgac cgccgacggg gccgtgcacg ggaccccggc ctacatgccc
     1801 ccggagcagt tcgccggcct cgccgccgac gcccgctcgg atcagt
//
LOCUS       DASWNJ010000224         1985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_180633, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000224 DASWNJ010000000
VERSION     DASWNJ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180633"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1985
                     /locus_tag="VGB85_04115"
     CDS             <1..>1985
                     /locus_tag="VGB85_04115"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY0133234.1"
                     /translation="TKLKVGFANQLRDIQVALPPDEPRPWDATDKLEVVGKPLPRIEA
                     PNKVTGAAKYTHDIRLPGLVHAAILRSPHPCATLGALDLEPARAAPGVVAVIALAKPG
                     DRLLFVGQDVAAVAAATPQQAREALSKISATYKPEPFVIATAAAMAPDAPRVHTGKVE
                     ERTTEGDEPGSSGGATQKGNIRMSPSQKKGDVDKGLRMAKVSHEATYTTQVHTHSALE
                     THSIVVRWDDAKRMTVWCSTQGIFSVRDEMAEIFELRPQDVHVITDYIGGGFGAKFGA
                     SAPGTRMGFVAGELARQAKAPVALLLDRREEHLCTGNRPDSIQKVTLGAAPDGTLTAI
                     KVVAHGTAGIGTGAGVGRNASGIYTKCPNIRVESHDVFTHAGPATAFRAPGHPQGAFA
                     LELALDELAAKLGKDPLDLRIAHNEHPVRRHQFELGRKQFEWDRRRKHASEQHKKGAR
                     VRHGIGVAASIWGDFGRGKAVTATVSVGRDGSVELRNGLQDIGGGITIVIAQVVAEVL
                     HRPVAEVRVKIGNSDLGSSVGSGGSNTTSSVTPAVRAAAEAVKTQLTTLAVQQLGARR
                     PEDVRWDSKGVSAGKKSITFTELCKHITGEAITATGTRPETYGAYPTKFAGGNSYQIA
                     GVQFAEVEVDTWTGLVRATRVLALHDCGRVMNEMTCKS"
BASE COUNT          346 a          784 c          614 g          241 t
ORIGIN      
        1 accaagctca aggtcggctt cgccaatcaa ctccgcgaca tccaggtcgc cctcccgccc
       61 gacgagccgc gcccctggga cgcgaccgac aagctcgagg tcgtcggcaa gccgctcccg
      121 cgcatcgagg ccccgaacaa ggtcaccggc gccgccaagt acacccacga catccgcctg
      181 cccggcctcg tgcacgctgc gatcctccgc agcccgcacc cctgcgccac gctcggcgcc
      241 ctcgacctcg agcccgcccg cgcggccccc ggcgtcgtcg cagtgatcgc gctcgccaag
      301 cccggcgacc gcctgttgtt cgttggtcaa gacgtcgccg ccgtcgccgc agccaccccc
      361 cagcaagccc gcgaagccct ctcaaaaatc tcggcgacct acaagcccga gcccttcgtg
      421 atcgccaccg ccgccgcgat ggcccccgac gccccgcgcg tgcacaccgg caaggtcgag
      481 gagcgcacca ccgagggcga cgagcccggc agcagcggcg gcgcgacgca aaagggcaac
      541 attcgcatgt cgccctcgca aaagaagggc gacgtcgaca agggcctgcg catggccaag
      601 gtcagccacg aggcgaccta caccacgcag gtgcacaccc acagcgcgct cgagacccac
      661 agcatcgtcg tgcgctggga cgacgccaag cgcatgaccg tgtggtgctc gacccagggc
      721 attttttcgg tgcgcgacga gatggcggag atcttcgagc tgaggccgca ggacgtgcac
      781 gtcatcaccg actatatcgg cggaggcttc ggcgccaagt tcggcgcgtc cgctccgggc
      841 acgcgcatgg gctttgtcgc cggtgagctc gcgcgccagg ccaaggcccc cgtcgcgctg
      901 ctgctcgacc gccgcgagga gcacctgtgc accggcaacc gcccggactc gatccaaaag
      961 gtcaccctcg gcgccgcgcc ggacggcacg ctgaccgcga tcaaggtcgt cgcccatggc
     1021 acagccggca tcggcaccgg cgccggcgtc ggccgcaacg cctcgggcat ctatacaaaa
     1081 tgccccaaca tccgcgtcga gagccacgac gtcttcaccc acgccggccc cgccacggcc
     1141 tttcgcgccc ccggccaccc gcaaggcgcc ttcgccctcg agctcgcgct cgatgagctc
     1201 gccgccaagc tcggcaagga cccgctcgac ctgcgcatcg cccacaacga gcaccctgtg
     1261 cgccggcacc agttcgagct cggccgcaag cagttcgagt gggacaggcg ccgcaagcac
     1321 gccagcgagc agcacaaaaa gggcgcccgc gtgcgccacg gcatcggcgt cgccgcttcg
     1381 atctggggcg acttcggccg cggcaaggct gtcaccgcga ccgtcagcgt cggccgcgac
     1441 ggcagcgtcg agctgcgcaa cggccttcaa gacatcggtg gtggcatcac catcgtcatc
     1501 gcgcaggtcg tcgccgaggt cctgcaccgc ccggtcgccg aggtccgcgt caagatcggc
     1561 aacagcgacc tcggctcctc ggtcggcagc ggcggcagca acaccacctc ctcggtcact
     1621 ccggccgtgc gcgccgccgc cgaggccgtc aagacccagc tgaccacgct cgccgtccag
     1681 cagctcggcg cgcgccggcc cgaggatgtc cgctgggaca gcaagggtgt atccgccggc
     1741 aagaagtcga tcaccttcac cgagctgtgc aagcacatca ccggcgaggc gatcaccgcc
     1801 accggcacgc ggcccgagac ctacggcgcc tacccgacca agttcgccgg cggcaacagc
     1861 tatcaaattg ccggcgtaca gttcgccgag gtcgaggtcg acacctggac cggcctcgtg
     1921 cgcgcgaccc gggtgctcgc gctgcacgac tgcggccgcg tcatgaacga gatgacctgc
     1981 aagtc
//
LOCUS       DASWNJ010000225         3157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_286793, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000225 DASWNJ010000000
VERSION     DASWNJ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286793"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..373)
                     /locus_tag="VGB85_04120"
     CDS             complement(119..373)
                     /locus_tag="VGB85_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133235.1"
                     /translation="MSETVTTAAVREAAGVSVVTVHRWAKLGLLPTPDKAFRGRRGTV
                     ALWPSHAPAQAKWVRERLDAGQTITQVLAELERGEFVRLD"
     gene            433..717
                     /locus_tag="VGB85_04125"
     CDS             433..717
                     /locus_tag="VGB85_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133236.1"
                     /translation="MKSIWYLEVIWNFTGMPTPPTSSQPQPCDGAPGRMARFILRDGT
                     TLEIHVPDATTVETGDSRARTAGFILRYGREIVDVGLLARLIRRQGPRAQ"
     gene            743..1369
                     /locus_tag="VGB85_04130"
     CDS             743..1369
                     /locus_tag="VGB85_04130"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEY0133237.1"
                     /translation="MFTPLLDDRVRLARGFVPPGRAPEVFDALLRDTPWLVVRYENAG
                     TPVELPRLTVNYGERSYDYSGLVFTPLPWTPLLTQLREQVELHAQARFNALIVQLYRD
                     GNDGVGWHADDSPGVGTDPTIASLSFGATRKFAVRRRDTRACVLELDLNAGDLLIMRG
                     DLQHTHQHRVPREPAVTAPRVNLTFRTLTGPAPQPSVPHTSRVTRWER"
     gene            complement(1370..2353)
                     /locus_tag="VGB85_04135"
     CDS             complement(1370..2353)
                     /locus_tag="VGB85_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0133238.1"
                     /translation="MAQTHFTPPGPLGFGGAPLGNMFGVVSEETAEAALVAAWETGVR
                     HFDTAPHYGSGLSEHRVGAVLRRHPREEFVLSTKVGRILRPDRSRPENPPFVQGLPFR
                     VEYDYSYDGAMRSLEHSCQRLGLAQIDVVYIHDIAADHHGDDWKQLFETAMNGAARAL
                     IRLRDEGVIKGWGLGVNLTEPCERALAMSDPDVFLLAGRYSLLNQPALDRLFPVCAER
                     GVHVVIGGPYNSGLLAGGRHFEYEEAPPEMVRKRDRIAEICDRHGIDLRSAALQFCAA
                     HPVVSAIIPGSKHADKVRENARLMAAPTPPALWDELRHEGLIPAEAPTPAG"
     gene            complement(2657..>3157)
                     /locus_tag="VGB85_04140"
     CDS             complement(2657..>3157)
                     /locus_tag="VGB85_04140"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY0133239.1"
                     /translation="RQFETAWELTQYHLAGLTTEACLWRPARAGLHVVQAPDGRWLAQ
                     WPEHEGYDLGPSSLAWLTWHLGFWWSMALDHALGDGTLTREDVTWPGTADEVRAWIGG
                     LQVKWRAMLEQLTDDDLRSPERTRWPFQDRPFGDVVAWANVELMKNAAEIGYARFLHA
                     VRDGEA"
BASE COUNT          473 a         1067 c         1151 g          466 t
ORIGIN      
        1 cttcaggtgg cgtgtggcgg acccgggagt ccgtcccagg ctagctttcg cgagatgaca
       61 gataatgccg gtgcctaagg aggatgcaac ggcgcttgac gcatggcccg tgccacaatc
      121 aatctagccg cacgaactca ccccgttcca gttctgcaag cacttgggtg atcgtctggc
      181 cggcgtcgag gcgttcgcgg acccacttcg cctgagctgg cgcatgggac ggccatagcg
      241 ccacggtgcc tcggcggccg cggaaagcct tgtccggcgt gggcaatagg ccgagctttg
      301 cccagcggtg cacggtcacc acggagaccc cggcggcctc gcgtacggcg gccgtggtga
      361 cggtttcgct cacgggcgga gtgtagcggc cctgcgcgag gcgccacaag acgacagggt
      421 tgtggtatgt ggatgaaaag catatggtat ctggaagtaa tctggaactt cacgggcatg
      481 ccgacgccac cgacctcctc ccagcctcag ccgtgtgacg gcgcgcccgg ccgaatggcg
      541 cgcttcatcc tgcgcgacgg caccaccctc gagatccacg tcccggacgc gaccacggtc
      601 gagactggag actccagggc ccgaaccgcg gggttcatcc tgcggtacgg gcgggagatc
      661 gtggacgttg gtctgctggc gcggctcatc cggcgccagg gtccgcgtgc ccaatgaccc
      721 cccgccgtgc cagcatggcc cggtgttcac gccgctcctc gacgaccgcg tgcggctcgc
      781 ccgcgggttc gtgccgcccg ggcgcgcgcc ggaggtgttc gacgcgctcc tgcgcgacac
      841 gccgtggctg gtggtgcgct atgaaaatgc cggcacgccg gtcgagctgc cgcggttgac
      901 cgtcaactat ggcgagcgat cgtacgatta ttcggggctg gtgttcacgc cgctgccgtg
      961 gacaccgctg ctcacgcagc tgcgcgagca ggtcgagctg cacgcgcagg cgcggttcaa
     1021 cgcgctgatc gtgcagctgt accgcgacgg caacgacggc gtcggctggc acgcggacga
     1081 cagccccggc gtggggacgg acccgacgat cgcgtcgctg tcgttcggcg cgacccggaa
     1141 gttcgcggtg cgacgcaggg acacgcgcgc gtgcgtcctc gagctcgacc tgaacgcggg
     1201 cgacctgctg atcatgcgcg gcgaccttca gcacacgcat caacacaggg tcccgcgcga
     1261 gcccgcggtg acggctccgc gcgtcaacct gacgtttcgg acgctcacgg ggcccgcacc
     1321 gcagccgagc gtgccccaca ccagcagggt cacccgctgg gagcgatgat cagccggcgg
     1381 gcgtcggagc ttcagcgggg atcaggccct cgtgccgcag ttcgtcccac agcgccggcg
     1441 gggtcggggc ggccatgagg cgcgcattct cgcggacctt gtccgcgtgc ttggagcccg
     1501 ggatgatcgc ggagaccacc ggatgtgcgg cgcagaactg gagcgcggcg gatcgcaggt
     1561 cgatgccgtg ccggtcgcag atctccgcga tgcgatcgcg cttgcggacc atctcgggtg
     1621 gcgcctcttc gtattcgaag tggcgcccgc ccgcgagcag accggagttg taggggccgc
     1681 cgatgaccac gtgtacgcca cgttcggcgc acaccgggaa cagccggtcc agcgcgggct
     1741 ggttgagcag gctgtagcga ccggcgagca ggaacacgtc ggggtccgac attgcgagcg
     1801 cgcgctcgca cggctcggtg aggttgacgc cgaggcccca gcccttgatc acgccctcgt
     1861 cgcgcagacg gatcagagcc cgggcggcgc cgttcatcgc ggtctcgaac agctgcttcc
     1921 agtcgtcgcc gtggtggtcg gcggcgatgt catgaatgta cacgacatcg atctgggcga
     1981 gcccgaggcg ctggcagctg tgctcgagcg agcgcatcgc gccgtcgtac gaatagtcgt
     2041 actcgacgcg aaatggcaga ccctgcacga acggcgggtt ctccggccgg cttcgatcgg
     2101 gccgcaggat ccggccgacc ttggtcgaga gcacgaattc ctcgcggggg tggcggcgca
     2161 ggacggcgcc gacgcggtgc tcggagaggc cgctgccgta gtgcggggcg gtgtcgaagt
     2221 gccgcacgcc cgtctcccag gcggcgacca gcgccgcctc ggccgtctcc tcggagacga
     2281 cgccgaacat gttgccgagc ggggcgccgc cgaagccgag cgggccgggc ggggtgaagt
     2341 gggtctgggc catcgtcgag cttgatgttg tcggtcgggc cggggagcaa cgcatccgcg
     2401 cccgcgaagg cgcccggccg gcccaggagc ggccgtcccg gatgccgcgc aggcaccgct
     2461 gcgggcgaac gattcgtcgc gcgtacgtgc cggagcgccg ggcctgcgac gatcaatacc
     2521 tgcgagtgac gcgacctggc gtccatcgac cacggccacg gcgcggtcac gaagcgtcgg
     2581 tgcacaccgg atatgcccgt gcacctcgat tgtcggctcc cggggacgga tgcgtgaaca
     2641 gctgcgatct gcatcgtcac gcctcgccgt cgcggaccgc gtggaggaag cgggcgtagc
     2701 cgatctcggc ggcgttcttc atcagctcga cgttggccca cgcgacgacg tcgccgaagg
     2761 ggcggtcctg gaacggccag cgggtgcgtt cgggggaccg caggtcgtcg tcggtcagct
     2821 gctcgagcat cgcgcgccac ttgacctgga gaccaccgat ccaggcgcgg acctcgtcgg
     2881 cggtgccggg ccaggtcacg tcctcgcggg tgagcgtgcc gtcgccgagc gcgtggtcga
     2941 gggccatcga ccaccagaag ccgaggtgcc aggtcaacca cgcgaggctc gacgggccga
     3001 ggtcgtaacc ctcgtgctcc ggccactgcg ccagccagcg cccgtcgggc gcctgcacca
     3061 cgtgcaggcc ggcgcgggcg gggcgccaca ggcacgcctc ggtggtgaga ccggcgaggt
     3121 gatactgggt gagctcccac gccgtctcga actggcg
//
LOCUS       DASWNJ010000226         2671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_215620, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000226 DASWNJ010000000
VERSION     DASWNJ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215620"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VGB85_04145"
     CDS             <1..734
                     /locus_tag="VGB85_04145"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HEY0133240.1"
                     /translation="LRAEDRAALPPRLAQAVDEVLPAVLAARARGPFEAGAWTVPAAA
                     LERHCDALGLVLARHRRPAARFTAWRRRGVLIAAVVVLGVLALRPWQSESFGPWRGTY
                     FNRMDFTGQSEVRRDLDLDFEWGNGSPMDVIPNDRFSARWDTCLTIPTTRQVAFQLIS
                     DDGSRLEIDGALVIDNWGKHTATTRGATLELAAGEHHLHVEYFEERSSASLTLLASLD
                     GEAPKPIPRTMLRAPDEGDENPCGE"
     gene            complement(764..>2671)
                     /locus_tag="VGB85_04150"
     CDS             complement(764..>2671)
                     /locus_tag="VGB85_04150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0133241.1"
                     /translation="SAETTGEPTTDTPTTTTDTPTTADTEEPTTGTPEPAVCGNGMQE
                     DGEECDNGADNADDKACTAACKANVCGDGLVGPGEGCDDGNQEDGDDCTNACAVQSCG
                     DGVVAKTEGCDDGNKDDTDECTANCTPAECGDGFVQAAIGEECDLKDQNSAEGVCTSE
                     CKNAVCGDGFVQLDVEACDDGKNNGDMGSCLADCSLNVCGDGKQSPAEPCDDGNKDVG
                     DGCNEACQLEDCGNNVVDAGEACDDGANGDNDDGCTDVCTVPACGDAFAQVSLMEECD
                     LGEGNKDSGDCTSTCKLPTCGDALVWENNEQCDNGDQNGDDQACKADCTDNICGDGFK
                     GPDEQCDDGNDVETDDCTTVCKNAACGDAFVQGAEECDLGPANADAGLCTLTCTNAKC
                     GDALVQAGVEDCDDGNMSNTDACVMGCKAAKCGDTFVNMGVEDCDDGNILSDDGCSAT
                     CKLENVAIGEKKMLGLAIPDDKYAGTQATMICTDLVIAKAGVIKGVQLVVGMDHSWIG
                     DLTFKLFSPANAKTLTVLSRPGLIEALDNGSDASFESSDFKKTAPVLFKDGGAKDAEK
                     IGDTILGTQTVCIDDGACEYFPNPGKGPGMKFADFNGAQAMGTWKFCVADGAPTDIGT
                     LDMVKLTLLLQ"
BASE COUNT          319 a          953 c          915 g          484 t
ORIGIN      
        1 ggctgcgcgc cgaggaccgc gccgcgctgc cgccgaggct ggcgcaggcg gtcgacgagg
       61 tgctgcccgc cgtgctcgcc gcgcgcgcgc gcgggccgtt cgaggcgggc gcgtggacgg
      121 tcccggccgc cgcgctggag cgccactgcg acgcgctcgg cctggtgctc gcgcgccacc
      181 gccgtccggc cgcgaggttc acggcgtggc gccggcgcgg cgtgctgatc gcggccgtcg
      241 tggtgctcgg cgtgctcgcg ctgcgcccgt ggcagtcgga gagcttcggc ccgtggcgcg
      301 ggacctactt caaccgcatg gacttcaccg gccagagcga ggtccgccgc gacctcgacc
      361 tcgacttcga gtggggcaac ggctcgccga tggacgtgat ccccaacgac cgcttctcgg
      421 cgcgctggga cacttgcctg acgatcccga cgacccgcca ggtcgcgttc cagctgatct
      481 ccgacgacgg ctcgcggctc gagatcgacg gcgcgctcgt gatcgacaac tgggggaagc
      541 acacggcgac aacccggggc gcgaccctcg agctcgcggc cggcgagcat cacctgcacg
      601 tcgagtactt cgaggagcgc agcagcgcga gcctgacgct gctcgcctcg ctcgacggcg
      661 aggcgccgaa gccgatcccg cgcaccatgc tgcgcgcgcc ggacgagggc gacgagaacc
      721 cctgcggcga gtgagccgca ggggtcctcc ggtcatcgcg tgatcactgc aacagcagcg
      781 tcagcttgac catgtcgagg gtgccgatgt cggtgggcgc cccgtcggcg acgcagaact
      841 tccaggtgcc catcgcctgc gcgccgttga agtccgcgaa cttcatgccg gggcccttgc
      901 cggggttcgg gaagtactcg cacgcgccgt cgtcgatgca gacggtctgg gtgccgagga
      961 tcgtatcgcc gatcttctcg gcgtccttgg ccccaccgtc cttgaacagg accggcgcgg
     1021 tcttcttgaa gtcggagctc tcgaaggacg cgtcggatcc gttgtcgagc gcctcgatca
     1081 ggccagggcg gctgagcacc gtcagcgtct tggcgttggc ggggctaaac agcttgaagg
     1141 tgaggtcgcc gatccaggag tggtccatgc cgaccaccag ctgcacgccc ttgatgacgc
     1201 cggccttggc gatcacgagg tcggtgcaga tcatcgtcgc ctgggtgccc gcgtacttgt
     1261 cgtcggggat cgccagcccc agcatcttct tctcgccgat cgccacgttc tcgagcttgc
     1321 aggtcgcgct gcagccgtcg tccgacaaga tgttgccgtc gtcgcagtcc tcgacgccca
     1381 tgttgacgaa ggtgtcgccg cacttggccg ccttgcaacc catgacgcag gcgtccgtgt
     1441 tgctcatgtt gccgtcgtcg cagtcctcga cgcccgcctg gaccagcgcg tcgccgcact
     1501 tggcgttggt gcacgtcagc gtgcacagac cggcgtcggc gttggccggg ccgaggtcgc
     1561 actcctccgc gccctgcacg aaggcgtcgc cgcaggcggc gttcttgcac accgtcgtgc
     1621 agtcgtcggt ctcgacgtcg ttgccgtcgt cgcactgctc gtcggggccc ttgaagccgt
     1681 cgccgcagat gttatcggtg cagtcggcct tgcacgcctg atcgtcgccg ttctggtcgc
     1741 cgttgtcgca ctgctcgttg ttctcccaca ccagagcgtc gccgcaggtc gggagcttgc
     1801 aggtcgaggt gcagtcgccg ctgtccttgt tgccctcgcc gaggtcgcac tcctccatca
     1861 ggctcacctg cgcgaaggcg tcgccgcagg ccggcacggt gcacacgtcg gtgcagccgt
     1921 cgtcgttgtc gccgttggca ccgtcgtcgc acgcctcgcc ggcgtcgacg acgttgttgc
     1981 cgcagtcctc gagctgacag gcctcgttgc agccatcgcc gacgtccttg ttgccgtcgt
     2041 cacacggctc ggccgggctc tgcttgccgt cgccgcagac gttgaggctg cagtcggcga
     2101 ggcacgagcc catgtcgccg ttgttcttgc cgtcgtcgca ggcctcgacg tcgagctgca
     2161 cgaagccgtc gccgcagacc gcgttcttgc actcggacgt gcacacgccc tcggccgagt
     2221 tctggtcctt gaggtcgcac tcctcgccga tcgcggcctg cacgaagccg tcgccgcact
     2281 cggccggcgt gcagttggcg gtgcactcgt cggtgtcgtc cttgttgccg tcgtcgcagc
     2341 cctcggtctt ggcgaccacg ccgtcgccac agctctggac cgcgcaggcg ttggtgcagt
     2401 cgtcgccgtc ctcctggttg ccgtcgtcgc agccctcgcc cggaccgacc agaccgtcgc
     2461 cgcagacgtt ggccttgcag gcggccgtac acgccttgtc gtcggcgttg tcggccccgt
     2521 tgtcacactc ctcgccgtcc tcctgcatgc cgttgccgca gaccgcgggc tccggggtgc
     2581 cagtcgtcgg ctcctcggtg tcggcggtgg tcggggtatc ggtcgtcgtg gtcggggtat
     2641 cggtcgtcgg ctcgcccgtg gtctcggcgg a
//
LOCUS       DASWNJ010000227         3030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_286822, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000227 DASWNJ010000000
VERSION     DASWNJ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286822"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="VGB85_04155"
     CDS             complement(<1..1271)
                     /locus_tag="VGB85_04155"
                     /inference="COORDINATES: protein motif:HMM:NF019255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1587 domain-containing protein"
                     /protein_id="HEY0133242.1"
                     /translation="MAHPSVNLVLAASLLTAGCYQGSASDSTAGNSGSTGPGGSADDG
                     PGSDGGSGDEGGELGPIVSEPLHRLNRLEYNNTVRDLLGTTLRPADSFPPDTVTDGFD
                     NVAEGLTLSPALMDLYATAARDLSTATLQPAPRWAAHIGAREHATATGQAGNAFDWGW
                     SMSRFQEGLAFSIDVPQAENVVLSILAGGDAFGKPTPEMGVLLDGVEVGHWLVTSLPT
                     APVVYSVPLALPPGPHSVVITFPNGSDQPAENIYNTLVVGYLDITSDTLVTPAGRALV
                     YICEPGAAPVPEDCYRTIVTHFTERAWRRPLTQAEADSVFALWQQLSATEGPDAAVGL
                     VVRAILMSAKFLYRPSFPGPGEAEGPEELVPLDPHVLASRLSYFLWSSMPDALLMSAA
                     ASGQLHTDEGMKAQVERMLADPRATGLRQGFA"
     gene            complement(1467..1706)
                     /locus_tag="VGB85_04160"
     CDS             complement(1467..1706)
                     /locus_tag="VGB85_04160"
                     /inference="COORDINATES: protein motif:HMM:NF023056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3253 domain-containing protein"
                     /protein_id="HEY0133243.1"
                     /translation="MSADLAAAILDLLALRRPGATICPSEVARAEAPQDWRPLMPQVR
                     AAAAKLAAAGRIEVTQGGVVVDPRTAHGPIRLRLP"
     gene            complement(1802..2851)
                     /locus_tag="VGB85_04165"
     CDS             complement(1802..2851)
                     /locus_tag="VGB85_04165"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0133244.1"
                     /translation="MSSDASIEVYDVVIIGAGIAGLVCARELRDAGRNVVVLDKSRGV
                     GGRCATRRMLGQPVDIGLSYYHADDPELLAALAAVPATKLPGWPHRIVGGGAPCHPAA
                     FRPGQRRLAFAEGVNVFPKHLARDLDLRLGTRVTRLDPMPRHVALEIEDGPPVYTRDV
                     VLTIPTPQAHAILPSAEGRSLRAVQALLGAVVIHPCITLVLGLPLDGPAPEFDILYPD
                     DSPIVQLIAHDSSKRDDPSYRVLVVQATAQWSREHLEDDRDVWRAALTQEAARLLGDW
                     VSEPAWSEVHRWRFAKTMPTTEMSAPQLFGLPGGGQLGLANEAFTLEAGVQGAYRAGK
                     RLAHKLLASPSRTSE"
BASE COUNT          434 a          972 c         1213 g          411 t
ORIGIN      
        1 gcgaagccct ggcgcaggcc ggtggcgcgc gggtcggcga gcatgcgctc gacctgggcc
       61 ttcatgccct cgtcggtgtg cagctggccg ctggcggcgg cggacatcag cagcgcgtcg
      121 ggcatgctgc tccacaggaa gtacgacagt cggctggcaa gcacgtgagg gtcgagcggg
      181 acgagctcct cggggccctc ggcctcgccg ggcccgggga agctcgggcg atacaggaac
      241 ttcgccgaca tgaggatcgc gcggacgacg aggccgacgg ccgcgtcggg gccctcggtg
      301 gcgctcagct gctgccacag ggcgaacacc gagtcggcct cggcctgggt cagcggacgt
      361 cgccaggccc gctcggtgaa gtgggtgacg atcgtgcgat agcagtcctc gggcaccggc
      421 gcggcgccgg gctcacagat gtacacgagg gcgcgaccgg caggcgtgac gagggtgtcg
      481 ctggtgatgt cgaggtagcc gacgacgagc gtgttgtaga tgttctcggc gggctggtcg
      541 gagccgttgg ggaaggtgat gacgaccgag tgcgggccgg gaggcagcgc gagcgggacg
      601 ctgtagacga ccggcgcggt cggcagcgag gtgacgagcc agtgcccgac ctcgacgccg
      661 tcgagcagca cgcccatctc gggcgtcggc ttgccgaacg cgtcaccacc cgcgaggatc
      721 gagagcacga cgttctcggc ctgcggcacg tcgatcgaga aggcgaggcc ctcctgaaaa
      781 cgagacatcg accagcccca gtcgaaggcg ttgccggcct gcccggtcgc ggtcgcgtgc
      841 tcgcgcgcgc cgatgtgcgc ggcccagcgc ggcgccggct gcaacgtggc agtcgagagg
      901 tcgcgcgccg cggtcgcgta gaggtccatc agcgcaggcg agagcgtcaa accctcggcg
      961 acgttgtcga agccatcggt gacggtgtcc ggcggaaagc tgtctgcggg ccgcaacgtg
     1021 gtgccgagca ggtcacgcac ggtgttgttg tattcgaggc gattgaggcg gtgcagcggc
     1081 tcgctgacga tcgggccgag ctcgccaccc tcgtcaccgc tgccgccgtc gctgccgggg
     1141 ccgtcgtccg ccgagccgcc cgggccggtg ctgcccgagt tgcccgccgt cgagtcggac
     1201 gcggagccct gataacagcc ggcggtcagc agcgaggcgg cgaggaccag gtttacggac
     1261 gggtgcgcca tgtttgatga ggatccccgg cgccgcgagg gacggcgccc acgaaagatt
     1321 gttaccaccg ttcgtgttgc ggcggatctg cgagatgggg gaatttgcag gcgatgctgg
     1381 cgccggagtg ggcatgtacg ttttcgcgcg gggtccgatc gtggtccgga gacccgggac
     1441 cggggcaccc acggcaccga tgcgattcac ggcaggcgca gtcggatcgg gccgtgggcc
     1501 gtccgcgggt cgacgacgac gccgccctgc gtgacctcga tgcgaccggc ggcggcgagc
     1561 ttcgccgcgg cggcccgcac ttgcggcatc agcgggcgcc aatcttgagg ggcctcggcg
     1621 cgggcgacct cggaggggca gatcgtcgcg cccggccggc gcagcgcgag caggtcgagg
     1681 atcgcggcgg cgaggtcggc ggacatgcgc gatacatagc accacgggtc gacgaggctg
     1741 caatgccagg atcgcggcga tatgtcgata gttcgcggat cgccggggca ggcgcggcgc
     1801 gtcactcgct ggtgcgcgag gggctggcga gcagcttgtg cgcgagccgc ttgccggccc
     1861 ggtaggcgcc ctggaccccc gcttcgaggg tgaaggcctc gttggcgagg ccgagctggc
     1921 cgccgccggg gaggccgaac agctgcgggg cgctcatctc ggtggtcggc atggtcttgg
     1981 cgaagcgcca gcggtggacc tcggaccacg cgggctcgct gacccagtcg ccgagcaggc
     2041 gcgcggcctc ctgcgtcagc gcggcgcgcc agacgtcgcg gtcgtcctcg aggtgctcgc
     2101 gcgaccactg agcggtcgct tgcacgacga gcacgcggta gctcgggtcg tcgcgcttgc
     2161 tcgagtcgtg ggcgatcagc tgcacgatcg gcgaatcgtc ggggtacagg atgtcgaact
     2221 cgggggcggg gccgtcgagc gggaggccga gcacgagggt gatgcagggg tgaatgacga
     2281 cggcgccgag cagcgcctgc acggcccgca gcgagcggcc ctcggcgctc ggcaggatcg
     2341 cgtgggcctg cggggtcggg atcgtcaaca cgacgtcgcg ggtgtacacc ggcgggccgt
     2401 cttcgatctc gagcgcgacg tggcgcggca tcgggtcgag gcgcgtgacc cgggtgccga
     2461 ggcgcaggtc gaggtcgcgg gcgaggtgct tggggaacac gttgacgccc tcggcgaagg
     2521 cgaggcgccg ctggccagga cgaaacgcgg ccgggtgaca cggcgcgccg ccgccgacga
     2581 tgcgatgcgg ccagccgggc agcttcgtgg ccgggacggc ggcgagggcg gcgagcagct
     2641 cggggtcgtc ggcgtggtag tacgacaggc cgatgtcgac cggctgaccg agcatgcgcc
     2701 gggtggcgca gcggcccccg acgccgcggc tcttgtcgag gacgacgacg ttgcggccgg
     2761 cgtcacgcag ctcgcgggcg cacacgagac ctgcgatccc cgcgccgatg atgacgacgt
     2821 cgtacacctc gatcgaagcg tcgctggaca tcaccgctag cgtgcgatcg tccggacggc
     2881 agggcgcagc ggcaggcagg cgtcggcgga tcggccggtt caggccgcgg cgggtcgctt
     2941 gcgacagatc cggtacgcag cccgcgcccc ggcgaattcg ctcacttgca ctcgccctgc
     3001 aggttctggc ggaagttgtt gaggccgggc
//
LOCUS       DASWNJ010000228         3981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_640612, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000228 DASWNJ010000000
VERSION     DASWNJ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640612"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1500)
                     /locus_tag="VGB85_04170"
     CDS             complement(<1..1500)
                     /locus_tag="VGB85_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133245.1"
                     /translation="MGDTGDTGATDVITGVPTSGTGDTSGTGDTGDTGDTGDMVTVYD
                     VEPGILASAVMPGFCEPEVYTKMVDGKPEYWLRDNVVGYADPRPRSFYVDYGYVGQTL
                     DYGPILRGGVYSVRCFRFAGELFWQQPQTNESHVVWFRVPGPMMEAGEVQVPASFQIE
                     DNFIVDQSKLLPNFAPIANKIAMVYLWRQKDTLDDIYGHGATHVATVGNPGEMDPAKL
                     GMTTAVHVNAPWMSQLGVNPTPDSMDGIGEAIFRTLSDAQIDEVVAQQPAVGLYSTDF
                     EKTPNYGDPNTYWSIDVEDATLPAYYRFLKKMKAAQPNRLVYDFYRALVWSKGFNSPG
                     DGNPDPSDPKWPPRLSDPEKYAAAPAYRKFDDGGSSVSLRDVIDLYTIDAYPYRGIGP
                     FDGSPDKSDRSWTSYQLYSMIYDTMIIKKLAPQSKVLFFGWSQSDNDQPTRFYIDLPT
                     GRASYMLRQPTPAAWAETVQMLGFIVGDGYHWWTEQIAKGDDPNLLGTGD"
     gene            complement(1796..3067)
                     /locus_tag="VGB85_04175"
     CDS             complement(1796..3067)
                     /locus_tag="VGB85_04175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0133246.1"
                     /translation="MSPQIAKNSEQGQAAPLMPAASGELMTDPSGPLSAYFRELAHTE
                     TMTREQEFVLATRIRDLRRSLWRATLNYPPFIAGICTLAQEVLGADGCPTEALEAMTV
                     AARKLRDRDLLIHRRAFESARETLTTAMAESDIDGQVIERVLADLIGIEGGQSSGLSL
                     KVKLPPRGSLPFLSYVSAVKVAHHAFWTARSEFVTANLRLVVTIARRYNRGRMPLQDL
                     VQEGNLGLMKAVDRFDPKKGCRFSTYGSWWIRHAVTRAIADKGRTVRLPVHMIDAYSK
                     VLRARREFETLHGRAASEPELAKACGVSPERLARMHVSLIEAPVSFDQRLSGGDLTLL
                     DAMEDTSQPAVPEALDQQLHMEHLQELFARLSPFEADILRRRVGLDDSPEMTLKEIGQ
                     HYGLSRERIRQLQEQALGKLRDEFRQRGLMG"
     gene            complement(3464..>3981)
                     /locus_tag="VGB85_04180"
     CDS             complement(3464..>3981)
                     /locus_tag="VGB85_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133247.1"
                     /translation="IALPFTRDGLSLLDVFLRIAADSPLAGLAFLIMFAAPQLFGLAV
                     AVARLVRDESIALALVQIPVAILQAMTVLFGMSLINNPKAVAPLALAGFALVSALYYL
                     YTSSEAAAADRPLRLRWYIRWGALLVAGLGMWLRLQALGSLRLGVALDVAVVTAVLLL
                     ASTAPRRPAIA"
BASE COUNT          619 a         1246 c         1464 g          652 t
ORIGIN      
        1 gtcgccggtg ccgagcaggt tggggtcgtc gcccttggcg atctgctcgg tccaccagtg
       61 atatccgtcg ccgacgatga agccgagcat ctgtacggtc tcggcccagg cggccggcgt
      121 cggctggcgc agcatgtacg aggcgcgccc ggtcggcagg tcgatgtaaa agcgcgtcgg
      181 ctgatcgttg tcgctctgcg accacccgaa gaacagcacc ttggactgcg gcgccagctt
      241 cttgatgatc atcgtgtcgt agatcatgct gtagagctgg tacgaggtcc acgagcggtc
      301 cgacttgtcg ggtgaaccat cgaagggccc gatgccgcgg tacggatagg cgtcgatcgt
      361 gtagaggtcg atgacgtcgc gcagcgacac cgaactgccg ccatcgtcga acttgcgata
      421 ggcgggggcc gccgcgtact tctcggggtc gctcaggcgc ggcggccact tcgggtcgga
      481 cgggtcgggg ttgccgtccc cgggcgaatt gaagcccttg gaccacacca gcgcccggta
      541 gaagtcgtat accagccggt tcggctgggc cgccttcatc ttcttcaaga accggtaata
      601 cgcgggcagc gtcgcgtcct cgacgtcgat gctccaatag gtgttcgggt cgccgtaatt
      661 gggggtcttc tcgaagtcgg tgctatagag gccgacggcc ggctgctgtg cgacgacctc
      721 gtcgatctgc gcgtcggaca gggtgcgaaa gatcgcctcg ccgatgccgt ccatgctgtc
      781 gggcgtgggg ttgacgccga gttggctcat ccacggggcg ttgacgtgga ccgccgtcgt
      841 catgccgagc ttggccgggt ccatctcgcc ggggttgccg acggtcgcca cgtgggtcgc
      901 gccgtgcccg tagatatcgt cgagcgtgtc cttttgccgc cagagataaa ccatggcgat
      961 cttgttggcg atcggcgcga aattgggcag caacttcgac tggtcgacga tgaaattgtc
     1021 ctcgatctgg aagctcgccg ggacctgcac ctcgcccgcc tccatcatcg gcccgggcac
     1081 gcggaaccat acgacgtggc tctcgttcgt ctgcggctgc tgccagaaca gctcgccggc
     1141 gaagcggaaa caacgcacgc tgtagacccc gccgcgcagg atcggcccgt agtcgagggt
     1201 ctggccgacg tacccgtaat cgacgtagaa cgagcgcggc cgcggatccg cgtagccgac
     1261 gacgttgtcg cgcagccagt actccggctt gccgtcgacc atcttggtgt atacctccgg
     1321 ctcgcaaaag cccggcatga cggcgctggc gaggatcccc ggctcgacgt cgtagacggt
     1381 caccatgtcg ccggtgtcgc cggtgtcgcc ggtgtcgccg gtgccactgg tgtcgccggt
     1441 gccactggtg ggcacgccgg tgatcacgtc ggtggcgcct gtatcgccag tgtctcccac
     1501 ggtggcggtg gcactggcgg tcgcctcggt ggtcggctga ctcgtggcgt cggtgccggt
     1561 cgctccggta tcggcggtac cgacagaacc gtccgaatcg gcgcggccct ccgagccgca
     1621 gcccgtgacc gccagaagga gagagagggt cgtcctgaag taatgccgtg ccaccctggc
     1681 agcctcgcag gaacggcgaa cgctggtcaa gccgcgcacc cgggggcggc tcgcagggcg
     1741 gggcgacggg ctccgggggc gcggagccgc cgacgtcacc gtcggcggac cggtgtcatc
     1801 ccatcaggcc gcgctggcgg aactcgtcgc gcagcttgcc gagcgcctgc tcctgcagct
     1861 ggcggatgcg ctcgcgcgac aggccgtagt gctggccgat ctccttgagg gtcatctcgg
     1921 ggctgtcgtc gaggccgacg cggcggcgca ggatgtcggc ctcgaacggg ctcaggcgag
     1981 cgaacagctc ctgcaggtgc tccatgtgca gctgctggtc gagcgcctcg gggaccgccg
     2041 gctgggaggt gtcctccatc gcgtcgagca aggtcaggtc gccgccggac aggcgctggt
     2101 cgaagctgac cggcgcctcg atcagggaca cgtgcatgcg ggcgagccgc tcggggctga
     2161 cgccgcacgc cttggccagc tcgggctcgc tggcggcgcg gccgtgcagg gtctcgaact
     2221 cgcgacgggc gcgcagcacc ttgctgtacg cgtcgatcat gtggaccggc aggcgcacgg
     2281 tgcggccctt gtcggcgatc gcgcgggtga cggcgtgacg gatccaccag ctgccgtagg
     2341 tcgagaatcg gcagcccttc ttcgggtcga agcggtcgac cgccttcatc agcccgaggt
     2401 tgccctcctg cacgaggtcc tgcagcggca tgcggccgcg gttgtagcgg cgggcgatcg
     2461 tgacgacgag gcgcaggttg gcggtcacga actcggagcg ggcggtccaa aacgcgtggt
     2521 gggcgacctt gaccgcggag acgtagctca ggaagggcag gctgccgcgc ggcgggagct
     2581 tgaccttgag gctcaggccg ctgctctggc cgccctcgat gccgatcagg tcggcgagca
     2641 cgcgctcgat cacctggccg tcgatgtcac tctcggccat cgcggtggtc aaggtctcgc
     2701 gggcggactc gaaggcgcgg cggtggatca gcaggtcgcg gtcgcgcagc ttgcgggccg
     2761 cgaccgtcat cgcctcgagc gcctcggtcg gacagccgtc ggcgccgagc acctcctggg
     2821 cgagcgtgca gatgccagcg atgaacgggg gatagttgag ggtcgcgcgc cagaggctgc
     2881 gacgcaggtc gcggatgcgc gtggcgagca cgaactcctg ctcgcgcgtc atcgtctcgg
     2941 tgtgggcgag ctcgcggaaa taggcgctca ggggacccga ggggtcggtc atcagctcgc
     3001 cggaggcggc gggcatcaat ggggcggcct gaccttgctc ggagtttttg gcgatctggg
     3061 gggacattgc tacctactcg ctatcggggg gtgaatgcgc tgtcccgtcg tgggcagcgc
     3121 agcacgaggc tgcgtccggc tccagagtgt gtcaaacctg tgttacaggt ttagtggtaa
     3181 tgcccaggcc aagattccca gggaagatca gcagggtagc gccagttttg ggtcgcgaga
     3241 cggggcttcg gggcgacttt ggggcacacg ccccccaatg tgaggccctg caatacgctc
     3301 atttctcgca gatccatggt caatggcagg agcgacacaa tgtgcgatgg accggcctgg
     3361 cggcacaggg ctcgcggtcg atcatcggac tgcgcgggtt ccgccgaact atgtgatcag
     3421 gaagtgatca gggttatccc tgttaactgt cccgaaggac aggtcaggcg atcgccggac
     3481 gacgcggcgc ggtgctggcg agcagcagca cggctgtgac gaccgccaca tcgagggcga
     3541 cgccgaggcg caggctgccg agcgcttgca ggcgcaacca catgccgagg ccggcgacga
     3601 gcagggcgcc ccagcggatg taccagcgca atcgcagcgg tcggtcggcg gccgcggcct
     3661 cgctcgaggt gtagagatag tacagcgcgg agacgagggc gaagccggcg agcgccagcg
     3721 gggcgacggc cttcgggttg ttgatcaggc tcatgccgaa cagcacggtc atcgcctgga
     3781 ggatcgccac cgggatctgg accagcgcga gggcgatcga ctcgtcgcgc acgaggcggg
     3841 cgaccgccac ggcgaggccg aacagctgcg gcgcggcgaa catgatcagg aaggcgaggc
     3901 cggcgagcgg gctgtcggcg gcgatccgca ggaacacgtc gagcaggctc aggccgtcgc
     3961 gcgtgaacgg cagggcgatc g
//
LOCUS       DASWNJ010000229         4284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_640656, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000229 DASWNJ010000000
VERSION     DASWNJ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640656"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VGB85_04185"
     CDS             complement(<1..420)
                     /locus_tag="VGB85_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133248.1"
                     /translation="MPRISSSLHLLPAFFFSFVASLTGCVLYGDAEETKSCDDVVCGN
                     NASCGGDQAECFCDAGHAGNPYEGCFAEMPVVDETCKAECGQNAYCSEGECYCELDHV
                     AVCGANAGCLPQERLCDTKQDCPDGADENVAVCNPPVF"
     gene            576..1895
                     /gene="bioA"
                     /locus_tag="VGB85_04190"
     CDS             576..1895
                     /gene="bioA"
                     /locus_tag="VGB85_04190"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:TIGR00508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEY0133249.1"
                     /translation="MATLRDRDRALLWHPATHFADLDRLPPIAVERAAGCWLYDDAGG
                     RILDAIASWWTCIHGHGHPAIVAAIAEQAARLDHVMFAGFTHAPAVELAAALLERAPP
                     GLGRVFFADCGSAAVEVALKLSYQRRQQTGEPGRTRFATLRNSYHGETLGALALCGSG
                     TYRDTFAPLLFSALELPAPAFPDHRPEQAASDLGADTPEADAAVAMLEQHAAELTAVV
                     VEPLVQCAGRMAMTGAGFYRRLAAAARALGIDVIADEIAVGFGRTGELFASTHAGVTP
                     DFLCLSKGLAGVLPLAAVLIRAGFEDAFTGAPARSFLHSHTFTANPIACAAALAGLTL
                     FDELRAGLPGRVRAMAERREAVAAACPQVLHHRQIGMISAFQIAPAGGPADGRLGLRL
                     REEALRRGVLLRPLNDTLYWMPPLVIDDAELDLLAEATIAAIHAALA"
     gene            1911..3182
                     /locus_tag="VGB85_04195"
     CDS             1911..3182
                     /locus_tag="VGB85_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133250.1"
                     /translation="MRRLVLVGCLAWAGGCISCTTGPTPDLALDMGSSCQSIALEVRD
                     DPVLPGWDVHALAVVRSGGEQAWALATNGKNQLRLKAWPDGPDHDLSGFGEPEDFRLI
                     PGPVEGQTWLTLEQPTQAGVWRFDEFDEEIVGGMVEGIPGDELARHRLVFLGDFPYLA
                     VIPRTGDASTMQMSLVQLDPDTLAPGTVFTLPLWTACFEDGYCNNALLTGPVEIDPIA
                     VTEAGLFGGAWMMIGIYGSRATEPPATYTLYMTRLLLVQLRSTGIDRPPQMLKVENFY
                     PPSTSPVVLDPAFLASDTSGFYWLAGLSTIEPSANTGSDLLFRLGSVGRYVTYEPIAS
                     AKRELRSHLLQIGGRAALGQIPGNTWTIAPLGADGVDTQLIAKLVIEPDAEISSAGRS
                     EFLVRGGGRARRVAAVCADDPKENDEEPEDE"
     gene            complement(3242..4063)
                     /locus_tag="VGB85_04200"
     CDS             complement(3242..4063)
                     /locus_tag="VGB85_04200"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HEY0133251.1"
                     /translation="MIRNSLLLVASFGLLAGCASSSANNPPPQDPDAGKVMYDAGGQP
                     QTGGTAGQTDPANGGAAADAPKGRDSTTTKAGGFKPKAVKKIAAAKPPKAETPVDPKG
                     TPTRKPRVDPNGLLVEAFTIEAAASKLPDFSGLKLSSIFIASTVDASATGPLAGAPAA
                     LKAPTALRFTGSLNVTASNEYKLCTKSSDGSQLYIEDTPVVDNDGVHADAVEKCELVA
                     LEPGEYKLEIRSFHVSGPIVVQASWAVGKDGTPTAIPKASLFKPEGADDRVKAKK"
BASE COUNT          599 a         1470 c         1570 g          645 t
ORIGIN      
        1 gaacaccgga ggattacaga cggcgacgtt ctcgtcggcg ccgtcggggc agtcctgctt
       61 ggtgtcacac agccgttcct gcggcagaca accagcgttc gccccgcaga cggcgacgtg
      121 gtcgagctcg cagtagcact cgccctccga gcagtaggcg ttttgcccgc actcggcctt
      181 gcaggtctcg tcgacgaccg gcatctccgc gaaacaaccc tcataggggt ttcccgcatg
      241 gccggcgtcg cagaagcatt cggcctggtc accgccgcag ctggcattgt tgccgcagac
      301 gacgtcgtcg cagctcttgg tctcctcggc gtcgccgtag agcacgcagc ccgtcaggga
      361 ggcgacgaag gagaagaaaa aggccgggag caggtgcaga gacgaggaga tgcggggcat
      421 ggcgaaccgt ccgtgacgct gcgcacggtc gcgatattcg cgaccgcgga ggggagtcga
      481 ggtgggtggg cacaagggtg cgcgacccgg gcccgcctgg caacggggat cgcaggttga
      541 cggcgagggc cccggcagac caggctgccg cggcgatggc gacccttcgt gaccgcgacc
      601 gcgccctgct gtggcacccg gcgacgcact tcgccgacct cgaccggctg ccgccgatcg
      661 ccgtcgagcg cgccgccggc tgctggctgt acgacgacgc gggcgggcgg atcctcgacg
      721 cgatcgcctc gtggtggacc tgcatccatg gccacggcca cccggcgatc gtcgccgcga
      781 tcgccgagca ggcggcgcgc ctcgaccacg tgatgttcgc cggcttcacc cacgcgcccg
      841 cggtcgagct ggccgcggcg ctgctggagc gggcgccccc gggcctcggg cgggtgttct
      901 tcgccgactg cggctcggcg gccgtcgagg tcgcgctcaa gctcagttat cagcggcgcc
      961 agcagaccgg cgagcccggg cgcacgcgct tcgccaccct gcgcaacagc taccacggcg
     1021 agacgctcgg ggcgctggcg ctgtgcggca gcggcaccta tcgtgacacc ttcgcgccgc
     1081 tgctgttctc ggcgctcgag ctgcccgccc cggcgttccc ggatcaccgg ccggagcagg
     1141 cggcgagcga cctgggggca gatacacccg aggccgacgc ggcggtggcg atgctcgagc
     1201 agcacgcggc ggagctgacg gcggtggtgg tcgagccgct ggtgcagtgc gcggggcgca
     1261 tggcgatgac gggcgccggg ttttatcgcc ggctcgccgc cgcggcgcgc gcgctgggga
     1321 tcgacgtgat cgccgacgag atcgcggtcg gcttcggtcg caccggcgag ctgttcgcct
     1381 cgacccacgc cggcgtcacg cccgacttcc tgtgcctgag caagggcctc gcgggggtgc
     1441 tgccgctggc ggcggtgctg atccgggccg gcttcgagga cgcgttcacc ggcgcgccgg
     1501 cccgcagctt tttgcacagc cacacgttca cggccaaccc gatcgcctgc gccgcggcgc
     1561 tcgcggggct gacgttgttc gacgagctgc gtgccgggct tccggggcgc gtgcgcgcga
     1621 tggcagagcg acgcgaggcg gtcgccgcgg cctgtcccca ggtgctgcat caccgccaga
     1681 tcgggatgat cagcgcgttc cagatcgcac ccgcgggcgg gcccgccgac gggcgcctgg
     1741 gcctgcgcct gcgcgaggag gcgctgcggc gcggggtgct gctgcgcccc ttgaatgaca
     1801 cgctgtactg gatgccgccg ctggtgatcg acgacgccga gctcgacctg ctcgccgagg
     1861 cgacgatcgc tgcgatccac gccgcgctcg cctgacgtat gctgaccccc atgcgccgcc
     1921 tcgtcctcgt cggctgtctc gcgtgggccg gtgggtgcat cagctgcacc acgggcccga
     1981 cgccggacct cgcgctcgac atgggcagca gctgccagtc gatcgcgctc gaggtccgcg
     2041 acgacccggt gctgccgggc tgggacgtgc acgcgctcgc cgtcgtgcgc agcggaggcg
     2101 agcaggcgtg ggccctggcg accaacggca agaaccagct gcgcctcaag gcctggcccg
     2161 acggccccga ccacgacctg tccggcttcg gcgagcccga ggactttcgc ctgatccccg
     2221 ggccggtcga ggggcagacc tggctgacgc tcgagcagcc gacgcaggcc ggcgtgtggc
     2281 gattcgacga gttcgacgag gagatcgtcg gcgggatggt cgagggcatc cccggcgacg
     2341 agctggcgcg ccaccggctg gtgttcctcg gcgacttccc ctacctcgcc gtgatcccgc
     2401 ggaccggcga cgcgagcacg atgcagatgt cgctcgtgca gctcgacccg gacacgctgg
     2461 cgcccgggac ggtgttcacg ctgccgctgt ggaccgcgtg cttcgaggac ggctactgca
     2521 acaacgccct gctgaccggc cccgtcgaga tcgacccgat cgcggtcacc gaggcggggc
     2581 tgttcggcgg ggcgtggatg atgatcggga tctacgggtc acgcgccacc gagccaccgg
     2641 cgacctacac gctgtacatg acccgcctgt tgctggtgca gctgcgatcg acgggcatcg
     2701 accgcccgcc gcagatgctc aaggtcgaga acttctaccc gccgagcacg tcgccggtgg
     2761 tgctcgaccc ggcgttcctc gccagcgaca cctccggctt ctactggctc gcggggctgt
     2821 cgacgatcga acccagcgcc aacaccggga gcgacctgct gtttcgcctc ggcagcgtcg
     2881 ggcgctacgt cacatacgag ccgatcgcca gcgccaagcg cgagctgaga tcccacctgc
     2941 tacagatcgg cggccgcgcg gcgctcgggc agatcccggg caacacctgg acgatcgcgc
     3001 cgctcggcgc cgatggcgtc gatactcaat tgatcgccaa gctggtgatc gaaccggacg
     3061 ccgagatctc gtcggccggt cgtagcgagt tcctggtccg cggcggcgga cgggcccgcc
     3121 gtgtcgcggc ggtctgcgcc gacgacccga aggagaatga cgaggagccg gaagacgaat
     3181 gagcgcgccg cggttgcccg cgacgcgctc acctgagacc gaacgatcgg ctcgcgtcga
     3241 cttacttctt ggccttcacg cggtcatccg cgccctcggg cttgaacagc gaggccttcg
     3301 ggatcgcggt cggggtgcca tccttgccga cggcccagct ggcctggacg acgatcggcc
     3361 cggagacatg gaacgagcgg atctcgagct tgtactcgcc gggctcgagc gcgaccagct
     3421 cgcacttctc gaccgcgtcg gcgtgcacgc cgtcgttatc gacgaccggc gtgtcctcga
     3481 tgtagagctg cgaaccatcg ctgctcttgg tgcacagctt gtactcgttg ctggcggtga
     3541 cgttgagcga gccggtgaag cgcagcgccg tcggggcctt gagggccgcc ggggcacccg
     3601 cgagcgggcc ggtggcgctg gcgtcgacgg tggaggcgat gaagatgctg ctcagcttga
     3661 gacccgagaa gtcgggcagc ttgctggcag ccgcctcgat cgtgaaagcc tcgacgagca
     3721 gaccgttggg gtcgacgcgc ggcttgcggg tcggcgtgcc cttcgggtcg accggcgtct
     3781 cggccttggg cggcttggca gcggcgatct tcttgacggc cttgggcttg aagcctccgg
     3841 ccttggtggt ggtcgagtcg cggcccttgg gcgcgtccgc agcggcaccg ccgttggcgg
     3901 ggtccgtctg accggcagta ccgccggttt gcggctggcc accggcgtcg tacatcacct
     3961 tgccggcgtc ggggtcttgg ggaggcggat tgttggcgga cgagctggca caaccagcca
     4021 ggagcccaaa actagcgacc aggagcagag aattgcggat catcggaaag tcctcgtgga
     4081 ggtcaggaaa tctggggccg gtagacgccg gggtccgtcg tgcgattcag ggggggaagt
     4141 gcgagccgtg gcggcggaca agtgtacggc gcgtggcccg atcggccagc atctttttgg
     4201 ccccagacgg gccaccgggc ccggaccgcc ggtcgtgatt gctgtcactg cgagaggggg
     4261 tcctcggcgg cgcagcgcgg gccg
//
LOCUS       DASWNJ010000230         4545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_534556, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000230 DASWNJ010000000
VERSION     DASWNJ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534556"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VGB85_04205"
     CDS             complement(<1..889)
                     /locus_tag="VGB85_04205"
                     /inference="COORDINATES: protein motif:HMM:NF019224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0133252.1"
                     /translation="MLHRTRPTFTRCASLLLLVALPGACGPRRDWAAFQEAFADAGTS
                     TGTSGESSTSGGPADADSHGGSTSEASSTTGTGSTESTDASAASETTGAADPGTSSSG
                     DPQGVCGDGIVALGEECDDANDVDTDECDNSCARAWFIFVTGDAVYTGDIHGIVGADT
                     RCRSRAGNALLPRHDKYRALISDSTTNAADRLHHARGYYRLVNGLPVAHGWDALMHDT
                     LENPIVVTEVSTTLKTSVWTGTLPGGTAVPGAAHCNDWLTNSPQVLGHYGSNAQVSAA
                     WINDPEPKVNPAPCIISGSI"
     gene            complement(889..1788)
                     /locus_tag="VGB85_04210"
     CDS             complement(889..1788)
                     /locus_tag="VGB85_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133253.1"
                     /translation="MTPTFALALCLSTAAVTTREPPSDLSPAARVHNHLGIAHIEAGA
                     YATGVDELDRAYMLMPDPLQYRAGRSKVLGSIRSALNHLYRSTGDPAYLRRLQTHLLR
                     HLEALLLALGDTATIDDTAGSLAALREVEQTLARHPVVAPAVVSRAAEPRRVPVVSQG
                     ATVPPPARPESDLSQAPKRRMRLAGGILLGAGVAAVGVSIAATVASLDNRNKLHSLAR
                     SLLTQDTARSLTAWQEGQRLYHRERDYQTVAILTGVAGGVMLATGVALRVLGKRRPSA
                     LARHHILPTRTTNTWALVFTGEF"
     gene            complement(1794..3197)
                     /locus_tag="VGB85_04215"
     CDS             complement(1794..3197)
                     /locus_tag="VGB85_04215"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0133254.1"
                     /translation="MHPGQHLAERYVVGERIGGGGMADVFRGHDLWLRREVAIKVIKH
                     GMASPEMCARMLQEGRAAAAIDHPHLLRVLDIGRIHASVYLITDLLHGRSLAEHLRGS
                     RDARLDWQEAIELLLPALDAIQRVHDHGYIHRDLKPDNLFVHHRDYEEALIVLDLGIA
                     KIAPALRGEGAPVPTETGRLLGTPAYMSPEQASSLPLDHRTDIYSIGVTLHRMLAGRL
                     PFEPGPGDTPYALMARHIYDTPPRVDHGCPDIPAALADIVLRTLAKAPAQRPQSMRDL
                     ASALRDCLPAPIVAIETPTQTLDRKRRVYHHKSLAGVIAVAALMFFQGPPANTAATTP
                     TLPDPLPDEPTPYIRTPIRIPAASAEITAIPARLAPEEAPPAPPPARSPAALARVLAA
                     AAGPIAGCIREHGDLDTRAITVTLTLRPAGQVAHADLVDRDETTLGRCVRPLLTRLRF
                     PAGPAQHIQHTFDRSPR"
     gene            complement(3464..3589)
                     /locus_tag="VGB85_04220"
     CDS             complement(3464..3589)
                     /locus_tag="VGB85_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133255.1"
                     /translation="MAMDGGWTLSVADPTNPVACLDNAALYCFESSRATRRRSPK"
     gene            complement(4010..>4545)
                     /locus_tag="VGB85_04225"
     CDS             complement(4010..>4545)
                     /locus_tag="VGB85_04225"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY0133256.1"
                     /translation="PGDMIRRVVERHYGEYADRNFPNAAARKDDLEQLAGYAERYPAA
                     SEFLGELALVQGISAENVLGAEAPDDMLVLSTIHQAKGLEWPVCFVLWLAEGRFPMAT
                     ALRQPSDIEEERRLFYVAATRAGDELYMSYPVIEETRDGPSHMLRPSKFLIEIDTQPA
                     VFERWQIEEAPAEPVPG"
BASE COUNT          655 a         1399 c         1772 g          719 t
ORIGIN      
        1 agatcgagcc ggaaatgatg cagggggcgg ggttgacctt gggctcgggg tcgttgatcc
       61 aggccgcgct gacctgggcg ttgctaccgt agtgaccgag gacctggggc gagttggtga
      121 gccaatcgtt gcagtgcgcg gcgccgggga cggccgttcc gcccggcagg gtgccggtcc
      181 acaccgatgt cttgagggtg gtgctgacct cggtgacgac gatcgggttc tcgagggtgt
      241 cgtgcatcag ggcgtcccag ccgtgggcga cggggaggcc gttgacgagg cggtagtagc
      301 cgcgggcgtg gtggaggcga tcggcggcgt tcgtggtgct gtcgctgatc agcgcccggt
      361 acttgtcgtg gcggggcaac agggcgttgc cggcgcggct gcggcagcgc gtgtcggcgc
      421 cgacgatgcc gtggatgtcg ccggtgtaga cggcgtcgcc ggtgacgaag atgaaccacg
      481 cgcgcgcgca gctgttgtcg cattcgtccg tatcgacgtc gttcgcgtcg tcgcactcct
      541 cgccgagcgc cacgatgccg tcgccgcaga cgccctgcgg gtcgccggag ctgctcgtgc
      601 ccgggtccgc ggcgccggtg gtctcgctcg cggcgctggc gtcggtgctc tccgtgctgc
      661 cggtgccggt ggtcgacgag gcttcgctcg tgctgccgcc gtggctgtcg gcgtcggccg
      721 gacctccgct ggtcgacgac tcgccgctgg tgccggtgct ggtgccggcg tcggcgaagg
      781 cttcctggaa cgcagcccag tcgcggcgtg gaccgcaggc gccgggtagc gcgaccagga
      841 ggaggaggga ggcgcagcgc gtgaaggtgg gtcgggtacg gtgcagcatc aaaattctcc
      901 ggtgaagacg agcgcccagg tgttggtggt ccgggtcggc aaaatgtgat ggcgggcgag
      961 cgcggagggc cggcgtttac cgaggacccg gagcgcgacg cccgtcgcga gcatcacgcc
     1021 gccggcgacg ccggtgagga tggcgacggt ttgataatcg cgttcgcggt gatacaggcg
     1081 ctggccttcc tgccacgcgg taagtgagcg ggccgtgtcc tgcgtgagca gggagcgcgc
     1141 gaggctgtgg agcttgttgc ggttgtcgag ggaggccacg gtcgcggcga tcgagacccc
     1201 gaccgcggcg acgccagcac cgaggaggat cccacccgcg agccgcatgc gccgcttcgg
     1261 cgcctgggac aggtcagact cgggccgggc cgggggtggg acggtcgcgc cttgcgacac
     1321 gaccgggacg cggcggggct cggccgcacg cgatacgacg gctggcgcca cgacggggtg
     1381 gcgcgccagg gtctgctcga cctcgcgcag cgccgcgagc gagccggcgg tatcgtcgat
     1441 cgtcgcggta tcgccgagcg cgaggaggag cgcctcgagg tggcggagca ggtgcgtctg
     1501 taatcggcgc agataggcgg ggtccccggt cgaccgatac aggtgattga gggcgctgcg
     1561 gatcgatccg aggaccttgc tgcggccggc ccgatactgc agggggtcgg gcatcagcat
     1621 gtaggcccgg tcgagctcat cgaccccggt ggcgtacgca ccggcctcga tatgcgcgat
     1681 gcccaggtga ttgtgcacgc gcgccgccgg cgagagatcg gagggtggct cgcgggtggt
     1741 gacggcggcg gtagacagac atagggcgag ggcgaacgta ggggtcatac gggttatctc
     1801 ggggagcggt cgaaggtgtg ctggatgtgt tgcgccggtc cggcggggaa gcgcaggcgg
     1861 gtcaggaggg gccggacgca tcggccgaga gtggtctcgt cgcggtcgac gagatcggcg
     1921 tgcgcgacct ggccggcggg gcgcagcgtc aatgtcaccg tgatcgcccg ggtgtcgagg
     1981 tcgccatgtt cgcgtataca accggcgatc ggtcccgcgg ccgctgcgag cacgcgcgcg
     2041 agcgcagcgg ggctccgcgc cgggggcggc gctggcggtg cctcctcggg cgcgagccgg
     2101 gcgggtatcg ccgtgatctc ggccgacgcc gcggggatcc ggatcggtgt gcgtatatac
     2161 ggcgtcggct cgtcgggcag cgggtcgggg agggtcggcg tcgtcgcggc ggtatttgcg
     2221 ggcgggccct ggaagaacat cagcgccgcg acggcgatga caccggcgag gctcttgtgg
     2281 tgatatacgc gccttttccg atcgagggtc tgtgtcggcg tctcgatcgc gacgatggga
     2341 gcgggcaggc agtcacgcag ggcggaggcg aggtcacgca tcgactgcgg gcgctgggcg
     2401 ggggccttgg cgagcgtacg caggacaata tcggccagcg ccgcggggat gtccgggcac
     2461 ccgtggtcga cgcgaggcgg ggtatcgtag atgtggcggg ccatcagcgc atacggggta
     2521 tcgccggggc cgggttcgaa cggcagccga ccggcgagca ttcggtgcaa cgtgacgccg
     2581 atcgagtaga tatcggtgcg atggtcgagc ggcaggctgc tggcttgctc gggggacatg
     2641 tacgccgggg tcccgagcag ccgtccggtc tcggtgggga ccggcgcacc ctcaccgcgc
     2701 agcgccgggg cgatctttgc gatccccaga tcgaggacga tcagcgcctc ctcgtagtcg
     2761 cggtggtgga cgaataaatt atccggcttg aggtcgcggt ggatataacc gtgatcgtgc
     2821 acccgctgga tcgcgtcgag cgccggcagg agcagctcga tcgcctcctg ccagtcgagc
     2881 cgggcgtcgc gtgacccgcg aagatgctcc gcgagcgatc tgccgtgcag gaggtcggtg
     2941 atcaaatata cgctggcgtg gatccggccg atgtccagca cacgcagcaa gtgtgggtgg
     3001 tcgatcgcag cggcggcgcg gccctcctgc agcatccgcg cgcacatctc cggcgaggcc
     3061 atcccgtgct tgatcacctt gatggcgacc tcgcggcgga gccacaggtc gtggccgcga
     3121 aacacgtccg ccatgccgcc gccgccgatc cgctcgccga ccacgtagcg ctccgcgagg
     3181 tgctgccccg ggtgcacggc tggcgggcag gcggcgagat cttgactgat ggggacgggg
     3241 aaggccgtcg acgctcgcgc aatgtcgatg gattgcatgg ccggctccgg tcaatcgccg
     3301 acgtgaatgc agtggagacg gtcggccgac gccgcgctgg tctccgggct atcacccgtg
     3361 cccgaaccgt cgccggaccc tgcgacgggc tcgacgcgga cgaccattgt cgtgaaagaa
     3421 gaagctaaca aaggtaatgt ggcagggcgt ggggtgaaaa aattcatttg ggagatctcc
     3481 tcctggtcgc tcgggatgac tcgaagcaat acagggcggc gttgtcgagg caggcgacgg
     3541 ggttggtcgg gtcggcgacg ctgagggtcc agccgccgtc catcgccacg ctgttgcccc
     3601 ggcagaccca ggactcccag ctcgtggacg tccaggccgg tcgaccctgc gttcggcgga
     3661 ccgtcgctgc cggggccggt ggccgatgct gccaggcggc ccaatcgcgc cccgagccgc
     3721 aggcggccgt ggccaggcgt ccagcacacg cggcgacgat gttcaacccg cccttcatgg
     3781 cgggacgttt tcacgtgcgt cctgtccgga gcaaggccga cgaggctcgg ccgcaacagc
     3841 gttacgtcag ggcagagaaa ttaatgtgtg ctcccttacc gaatctgacg ccggcaatgg
     3901 atgtcccggg tagtttccag gatccaccac aaagcgcgga ggtccgcctc gccgtccgtc
     3961 ggccagagat cgtagagccc gcgggcaatg acggcaccgc ggcgcggggt cagcctggca
     4021 ctggctcggc gggcgcttcc tcgatctgcc agcgttcgaa cacggctggt tgcgtgtcga
     4081 tctcgatcag gaatttgctc gggcgcagca tgtgcgaggg gccgtcgcgg gtctcctcga
     4141 tcaccggata gctcatgtac agctcgtcgc cggcccgcgt ggcggcgacg tagaacaggc
     4201 gccgctcctc ctcgatgtcc gagggctggc gcagcgcggt cgccatcggg aatcttccct
     4261 cggcgagcca cagcacgaag cacaccggcc actcgaggcc cttggcctgg tgaatggtcg
     4321 acagcaccag catatcgtcc ggcgcctcgg cgccgagcac gttctcggcg ctgatgccct
     4381 gcaccagcgc gagctcgccg aggaattcgc tggcggccgg gtagcgctcg gcgtagccgg
     4441 cgagctgctc gaggtcgtcc ttgcgcgcgg cggcgttggg gaagttgcga tcggcgtatt
     4501 caccgtagtg gcgctcgacg acgcggcgga tcatgtcacc cgggg
//
LOCUS       DASWNJ010000231         3716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_180792, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000231 DASWNJ010000000
VERSION     DASWNJ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180792"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VGB85_04230"
     CDS             <1..612
                     /locus_tag="VGB85_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133257.1"
                     /translation="HLQLAAHHTNATRPSPPATTGAPDPAELHRPLCAADTLVLRHRA
                     RVRAIGRASPAFESTIADLRGELHLRLAGAQCPAEVPGEAVAVPVAGVASTGLRPDLL
                     LRPGQLSAETRERRPPPPSGPRWRHAKRGGVAMMTLGLMALGGGIAAGAVDIEHQGAL
                     QGGLLVAGTAMFVGGFPLLIIADQRAREAAVLAVGPGGARLRF"
     gene            715..1467
                     /locus_tag="VGB85_04235"
     CDS             715..1467
                     /locus_tag="VGB85_04235"
                     /inference="COORDINATES: protein motif:HMM:NF016341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(His) guanylyltransferase Thg1 family
                     protein"
                     /protein_id="HEY0133258.1"
                     /translation="MRSDDLAARMHTHETAGDDCVAAEVYTVARLDGRGFSRLARRPE
                     LEPPLDPRVRDVMIATADHLMRCGFEVAYAYTQSDEISLLLRRDDQTANPSLRRLLAV
                     LVGEASAKATLLFGELACFDCRLSALADVDAVVDHFRWRQAEATDEAIAAHCRFALRE
                     AGASEAEAAGRLEGLDLAQRQALLATLGVDFERLPAWQKYGAGLQWVQLDVDPTRTTL
                     LRRLRVELELPHGECYAAFVREQVGRARAPGT"
     gene            1788..2621
                     /locus_tag="VGB85_04240"
     CDS             1788..2621
                     /locus_tag="VGB85_04240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0133259.1"
                     /translation="MTNTRAVALGDISETLMIPLWCRAIETQRRGGVLRDPRAREIVA
                     SLDYDFDRRFAAHRDLAFRACLRTVSIDRWAREFLHLHPGGTVVEIGTGLNTRFERID
                     NGALRWFDVDLADAIALRRRFFADTPRRTMIAGAFGESDWLAQLASERGGPYLFIAEA
                     VLVYLPEAVVRRGLAAIATAFPGSQFVFDTVTPAAIAAQARPMMQTYRAPFVWGCDDA
                     RAIESWGAYRRLDSTTMFDLPPELRRRVPLRHRLALPYLRLLRPEVLRSHALHRFELR
                     A"
     gene            2645..3472
                     /locus_tag="VGB85_04245"
     CDS             2645..3472
                     /locus_tag="VGB85_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133260.1"
                     /translation="MTDPRSTSHSFTLLFTGLVLAACGAPADATGGSDTSSSSATTDA
                     PDPTTGEAGDTSSSGEGGDPGEAQCQADLQDCPDDFKCVLRRGAADWEFVCLPVQGEH
                     AAGEACHHDGVIAGTDDCDQSSWCIGAFDTTGNAWDGLCYPLCVGMTCEAEQRCVGIG
                     ALPVCAPTCDPLVGGDCGSDEACIYRPPEGFVCFPTGSDGAAIGEPCETAVSCDAGLH
                     CAQKVAGCAPEDFCCTDYCDTGSASNTCAQQAAGASCATIGATDPTQAHVGACVVQD"
     gene            3489..>3716
                     /locus_tag="VGB85_04250"
     CDS             3489..>3716
                     /locus_tag="VGB85_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133261.1"
                     /translation="MLGEPELFVAEPHARALISSLLRELPGWVEVEACGRLRLELALT
                     NATIEVPLQRPSGVGRVASGQPRLRDAAGVRA"
BASE COUNT          479 a         1341 c         1383 g          513 t
ORIGIN      
        1 cacttgcagc tcgcggccca ccacacgaac gcgacccggc cgtcgccgcc ggcgaccacg
       61 ggcgcgccgg acccggccga gctgcaccgt ccgctgtgcg ccgccgacac gctcgtgctg
      121 cgtcaccgcg cccgcgtgcg cgcgatcggc cgcgcaagcc cggccttcga gtcgacgatc
      181 gccgacctgc gcggcgagct gcacctgcgc ctcgccggcg cgcagtgccc ggccgaggtc
      241 ccgggcgagg cggtcgccgt cccggtcgcc ggcgtggcga gcacggggct tcgtcccgac
      301 ctgctccttc ggccaggtca gctgagcgcc gagacccgcg agcgccgccc gccgccgccg
      361 agcggaccgc gctggcggca cgccaagcgc ggcggggtgg cgatgatgac gctcggcctg
      421 atggccctcg gcggtgggat cgcggcgggc gccgtcgaca tcgagcacca gggcgcgttg
      481 cagggcgggc tgctcgtcgc cggcaccgcg atgttcgtcg gcggctttcc gctgctgatc
      541 atcgccgacc agcgcgcccg cgaggccgcc gtgctcgcgg tcggtccggg cggcgctcgc
      601 ctgcggtttt gatcggccgg tgcaggtaca cgggcgccgg aggcccgtgg cggccagggc
      661 ccggcgcgcc cgaaccccgc ccgcgcgcga gcgccctgtg gtagaccggg cgcgatgcgc
      721 tcggacgacc tcgcggcgag gatgcacacc catgagaccg cgggcgacga ctgcgtggcg
      781 gcggaggtgt acacggtggc gcggctcgac gggcgcgggt tctcgcggct ggcgcgtcgt
      841 cccgagctcg agccgccgct cgacccgcgc gtgcgcgacg tgatgatcgc caccgccgat
      901 cacctgatgc gctgcggctt cgaggtcgcc tacgcgtaca cccagagcga cgagatctcg
      961 ctgctgctgc gccgcgacga tcagaccgcg aacccgtcgc tgcgccgctt gctggcggtg
     1021 ctcgtcggcg aggcgagcgc gaaggcgacg ctgctgttcg gcgagctcgc gtgcttcgac
     1081 tgccgactct cggcgctggc ggacgtcgac gcggtcgtcg accacttccg ctggcggcag
     1141 gccgaggcga ccgacgaggc gatcgcggcg cactgtcgct tcgcgctgcg cgaggcgggg
     1201 gcgagcgagg ccgaggccgc gggtcgcctc gagggcctcg acctcgcgca gcggcaggcg
     1261 ctgctcgcca cgctcggcgt cgacttcgag cgcctgccgg cctggcagaa gtacggggcc
     1321 ggcctgcagt gggtgcagct cgacgtcgat ccgacgcgga cgacgctgct acgacggctc
     1381 cgcgtcgagc tcgagctgcc gcacggcgag tgctatgcgg cgttcgtgcg cgagcaggtc
     1441 ggccgggccc gcgcgcccgg gacctgatcg cgcgacttca ctgcgggatg ccgtcgaggc
     1501 cgcccgggta cccgtcgctg ccgtagctgt ggatccgtac acgccgacgc cctgtccgcg
     1561 gtgatgttgt ggtcgccgtc gctgcacgaa tagccgccgt cgccgatcac tccagccgcc
     1621 cgccgcggcg ccgcacgctg gtgccggccc tgaccatccg gtcgttgcag caggggcttc
     1681 ctccggccgg atccgcgtgg atccacgcat ttaggcaaat ctcggcgttc caggttgact
     1741 ttgaaaatta aaatcaataa acagggcagg tgagaacgga gccgggcatg acgaacacgc
     1801 gagcggtggc gctgggcgac atcagcgaga ccttgatgat cccgctgtgg tgtcgcgcga
     1861 tcgagacgca gcgacggggc ggcgtgctgc gcgatccgag ggcgcgagag atcgtggcga
     1921 gcctcgacta cgacttcgac cggcgcttcg ccgcgcaccg cgacctggcg tttcgcgcgt
     1981 gcctgcgcac ggtgtcgatc gaccgctggg cgcgcgagtt cctgcacctg caccctggcg
     2041 gcacggtggt ggagatcggc acggggctca acacgcgctt cgagcggatc gacaacggcg
     2101 cgctgcgatg gttcgacgtc gacctcgccg acgcgatcgc gctgcgccgg cgcttcttcg
     2161 ccgacacgcc gcgacggacg atgatcgccg gggccttcgg cgagtccgac tggctggcgc
     2221 agctcgccag cgagcgcggc ggaccctacc tgttcatcgc cgaggcggtg ctcgtgtacc
     2281 tgcccgaggc cgtggtgcgc cgcggcctcg cggcgatcgc gaccgccttc cccggcagtc
     2341 agttcgtgtt cgacaccgtg acgcccgcgg cgatcgccgc ccaggcgcgg ccgatgatgc
     2401 agacctaccg cgcgcccttc gtgtggggct gcgacgacgc ccgcgcgatc gagtcgtggg
     2461 gcgcgtatcg ccggctcgac tcgacgacga tgttcgacct gccgcccgag ctgcgccgcc
     2521 gcgtgccgct gcgccaccgg ctcgcgctgc cgtacctgcg cctgctgcga cccgaggtgc
     2581 tgcgctcgca cgcgctgcac cgcttcgagc tacgcgcctg acttcaggcc ccggagacac
     2641 ttccatgacg gacccacgat cgacctcgca ttcgttcacc ctcctcttca ccggcctcgt
     2701 gctcgcggcc tgcggcgcgc ctgccgacgc gaccggcgga tccgacacgt cgagcagctc
     2761 ggcgacgacc gacgcgcccg atccgacgac cggcgaagcc ggcgacacca gcagcagcgg
     2821 cgagggcggc gatccaggcg aggcccagtg tcaggccgat ctgcaggact gcccggacga
     2881 cttcaagtgc gtgctgcgac gcggcgccgc cgactgggag ttcgtgtgcc tgccggtgca
     2941 gggtgagcac gcggcgggcg aggcgtgtca ccacgacggc gtgatcgccg gcaccgacga
     3001 ctgcgatcaa agctcgtggt gcatcggcgc gttcgacacg acgggcaacg cgtgggacgg
     3061 cctgtgttac ccgctgtgcg tcggcatgac ctgcgaggcc gagcagcgct gcgtcggcat
     3121 cggcgcgctg ccggtgtgcg caccgacctg cgatccgctg gtcggtggcg actgcggcag
     3181 cgacgaggcg tgcatctacc ggccgcccga gggcttcgtg tgcttcccga ccggcagcga
     3241 cggcgccgcg atcggcgagc cgtgcgagac cgcggtcagc tgcgacgccg gcctgcactg
     3301 cgcgcagaag gtggcaggct gcgcgccgga ggacttttgc tgcacggatt actgtgacac
     3361 cggcagcgca agcaacacct gcgcgcagca ggccgcaggg gccagctgcg cgacgatcgg
     3421 cgcgacggac ccgacgcagg cgcacgtcgg cgcgtgcgtg gtccaggact agaagggggc
     3481 aggggacgat gctgggtgaa ccggagctgt tcgtggccga gccgcacgcg cgcgcgctga
     3541 tcagcagcct gctgcgcgag ctgcccggat gggtggaggt cgaggcgtgc gggcggctgc
     3601 ggctcgagct ggcgttgacc aatgcgacga tcgaggtgcc gctgcaacga ccctccggcg
     3661 tcggccgggt cgccagcggt cagccgcgcc tgcgcgacgc ggccggtgtt cgcgcg
//
LOCUS       DASWNJ010000232         2332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_39888, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000232 DASWNJ010000000
VERSION     DASWNJ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39888"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VGB85_04255"
     CDS             <1..393
                     /locus_tag="VGB85_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0133262.1"
                     /translation="QADNNRYQGQFLIYVLPRGAGGRIEPSYFANLSLSYAYPLPGEL
                     ELEFTARLANLTNNKAVLRVDETYSFSYGRAIAGGDLEDLKHSKIQNANAPTSFFQRG
                     VLPRQGNFGVQTVFQQPIAAQFELRLRF"
     gene            692..1900
                     /locus_tag="VGB85_04260"
     CDS             692..1900
                     /locus_tag="VGB85_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133263.1"
                     /translation="MFSSACGDSSAGGETADTGGSSGAGTSAPTGGTDTAEPTTGLTG
                     GASTTGTTGTTGGASQGGTDTGESTTTGESSGGPAPDMGGPPASFGRCGDEPPPGATL
                     APEIPTYAGMCPILETGYMDGQAPNIIVQDIEGLKLDRSFLVIVPDDMQPDEKLPVMF
                     MWHWLGGSSKDFYERADAQAAVNEKRFIAVIPDGREAEQGAPFKWPFSILDPDASMEA
                     DFQLFEDMLSCVAEQFAVDKDCVSSVGVSAGALFTSQLAGHRGERLASFMSLSGGTGG
                     NVVKGWNEPQHKMPALVLWGGEDDFCIAVDFQQTSQDLEKHLVEGGHFVVECIHNCNH
                     STPPFEVPEGMTAFAPLWDFLLDHPYWLKAGESPYNDAGLPISMPAWCAVGVGNAVPP
                     AGTCDKNECS"
     gene            1905..>2332
                     /locus_tag="VGB85_04265"
     CDS             1905..>2332
                     /locus_tag="VGB85_04265"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0133264.1"
                     /translation="MTRTRETLSVPPTRALLLVLSLAACADDGAAAATSTDAQGSTGS
                     TGSTSSTTSTSVVTGTSSGEAPPTSGDMTSTGVAGTSTGEPGFDPPRPACGNGYLEAM
                     EECDDGNLVDGDGCNAACQTPCGLELELLELAPTAESTISG"
BASE COUNT          371 a          857 c          788 g          316 t
ORIGIN      
        1 caggcggaca acaaccgcta ccagggtcag ttcctgatct acgtgctgcc gcgcggcgcc
       61 ggtggccgca tcgagccgag ctacttcgcc aacctgagcc tcagctacgc ctacccgctg
      121 cccggcgagc tcgagctcga gttcaccgcc cgcctcgcca acctcaccaa caacaaggcg
      181 gtgctccgcg tcgacgagac ctactcgttc tcgtacggcc gcgcgatcgc cggtggtgac
      241 ctggaagacc tgaagcacag caagatccag aacgcgaacg cgccgacgag cttcttccag
      301 cgcggcgtcc tgccccgtca gggtaacttc ggcgtgcaga ccgtattcca gcagccgatc
      361 gccgctcagt tcgagctgcg cctgcgcttc tgatccgaca gcccctcacc gggcgaacga
      421 gagggcggtc cagtcgggcc gccctttttg cgtcggtggc ctcgacccgc accggcctgc
      481 cgtactggac cgacaagtac aacgacgcga tcgacctcca cgacggcgtc tccttcgtgc
      541 tgtcaccctg ccgatcgtcg cggccggcac cgacgacgtc gccaggcacg ggtccagcca
      601 gtcaatccgg gccacgcgcc ttgaccggcg cgggccggat ctgcgatcgt gcgctgtgct
      661 gccgcgaacg cgtcccctcc tggtcctggc gttgttctcc tccgcctgcg gtgacagctc
      721 cgcgggcggc gagacggccg acaccggcgg atcgtcgggc gcgggcacga gcgcgccgac
      781 gggcggcacc gacaccgccg agccgacgac cggcctgacg ggaggcgcca gcacgacggg
      841 cacgacgggc acgaccggtg gcgcctcgca gggcgggacc gacacgggcg agtcgacgac
      901 cacgggcgag agcagcggcg gaccagcgcc ggacatgggc gggccgccgg cgagcttcgg
      961 gcgctgcggc gacgagccgc cgcccggcgc gacgttggcc ccggagatcc cgacctacgc
     1021 cggcatgtgc ccgatcctcg agaccggcta catggacggc caggcgccca acatcatcgt
     1081 ccaggacatc gagggcctca agctcgaccg cagcttcctc gtcatcgtgc ccgacgacat
     1141 gcagccggac gagaagctcc cggtgatgtt catgtggcac tggctcggcg ggtcgagcaa
     1201 ggacttctac gagcgcgccg acgcccaggc ggccgtcaac gagaagcgct tcatcgccgt
     1261 catccccgac ggtcgcgagg ccgagcaggg cgcgccgttc aagtggccgt ttagcatcct
     1321 cgaccccgac gcctcgatgg aggccgactt ccagctgttc gaggacatgc tgtcgtgcgt
     1381 cgccgagcag ttcgcggtcg acaaggactg cgtcagcagc gtcggcgtca gcgcgggtgc
     1441 gctgttcacc agccagctcg cgggccaccg cggcgagcgg ctggcctcgt tcatgtcgct
     1501 ctccggcggc accggcggca acgtggtcaa gggctggaac gagccgcagc acaagatgcc
     1561 ggcgctggtg ctgtggggcg gcgaggacga cttctgcatc gcggtcgact ttcaacagac
     1621 ctcgcaggac ctcgagaagc acctcgtcga gggtgggcac ttcgtggtcg agtgcattca
     1681 caactgcaac cattcgacgc cgcccttcga ggtgcccgag ggcatgacgg ccttcgcccc
     1741 gctgtgggac ttcctgctcg accacccgta ctggctcaag gccggcgagt cgccctacaa
     1801 cgacgccggc ctgccgatca gcatgccggc gtggtgcgcc gtcggggtcg gcaacgcggt
     1861 cccgcccgcc ggcacctgcg acaagaacga gtgctcgtga tcccttgacg cgcacccgcg
     1921 aaaccctgtc cgtgccgccc acccgcgccc tgctgctcgt cctctcgctc gcagcctgcg
     1981 ccgacgacgg cgcagccgcg gcgacgagca cggacgcgca gggcagcacc ggcagcaccg
     2041 gcagcacgtc gagcacgacg agcacctcgg tggtgacggg cacgtcgagc ggtgaggcgc
     2101 cgccgacgag cggcgatatg acgagcaccg gcgtcgcggg cacctcgacc ggcgagcccg
     2161 gcttcgaccc gccgaggccg gcctgcggca acggctacct cgaggcgatg gaggagtgcg
     2221 acgacggcaa cctcgtcgac ggcgacggct gcaacgcggc gtgtcagacc ccgtgcggcc
     2281 tcgagctcga gctgctggag ctggccccga ccgccgagtc gacgatctcc gg
//
LOCUS       DASWNJ010000233         1629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_391487, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000233 DASWNJ010000000
VERSION     DASWNJ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391487"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..358)
                     /locus_tag="VGB85_04270"
     CDS             complement(59..358)
                     /locus_tag="VGB85_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0133265.1"
                     /translation="MSLTQLGYFVAVAEEQHVGRAARRLHVSQPPLSRQIRSLEDELG
                     APLFTRTPRGVRLLPAGERFLGHARAILAAVDEARHAVVDPIAAPPGPPVPVDRR"
     gene            570..1457
                     /locus_tag="VGB85_04275"
     CDS             570..1457
                     /locus_tag="VGB85_04275"
                     /inference="COORDINATES: protein motif:HMM:NF024267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0133266.1"
                     /translation="MTELDIVEGLRGRIAELERELGRHAETERARAMSVSLLQATLEA
                     TTDGLLVVDLAGRVTSANGRFVELWRIPQALLATGDDQRVLAHVIEQLADPAAFTAKV
                     AALYGDRLAESFDLLEFKDGRAFERYSRPQLLDGEPVGRVWSFRDVTERRRAEQEAER
                     SKLQDQVIAAQRLVLSQLSTPLIPISAEVLVMPLIGTIDDERARQIVATLLDGIARTR
                     ARVVIVDITGVAGVDTQVAGGLLRAAQAVKLLGAQVVLTGIRPEVAQTLVSLGVDLGG
                     LVTRSTVQTGIAYATERRG"
BASE COUNT          224 a          572 c          612 g          221 t
ORIGIN      
        1 gtgcgacgtc ggcgacaagc tgccccgctg aagtcccgcg ccggccgatc ggcgtcgact
       61 atcgcctgtc cacagggaca ggtggtccgg gcggcgctgc gatcggatcg acgaccgcgt
      121 ggcgggcctc gtcgaccgcg gcgaggatcg cccgcgcgtg cccgaggaac cgctcgcccg
      181 cgggcaacag ccgcacgccc cgcggcgtgc gggtgaacag cggcgcgccg agctcgtcct
      241 cgaggctgcg gatctgccgg ctcagcggcg gctgcgagac gtgaaggcgt cgcgcggcgc
      301 gacctacatg ttgctcctcg gcgacggcga cgaagtagcc cagctgcgtg aggctcacgc
      361 ccgcagcgta gagcacgccc cgccgcgcga gcaatcccga gatcaaaaag gtatcgggct
      421 cgaacccggc tgccgggctc gaccttcgca acgacggtcg gcgtcggcga gcgctgcgcc
      481 ccttctcaga acggggatac tggtgggctg gcgcccccat ctgctagctt actggggggg
      541 ttgatacccc gggtgtcgcg ttgaccacgg tgacagagct ggacatcgtc gagggcctgc
      601 gcgggcggat cgccgagctc gagcgcgagc tcgggcgtca cgcggagacc gagcgcgcgc
      661 gggccatgtc ggtctcgctg ttgcaggcga cgctcgaggc gacgacggac gggctgctcg
      721 tcgtcgacct cgccggcagg gtcaccagcg ccaacggccg cttcgtcgag ctgtggcgga
      781 tcccgcaggc gctgctcgct accggcgatg accagcgggt gctcgcccac gtgatcgagc
      841 agctggccga tcccgcggcc ttcaccgcca aggtggccgc gctgtacggc gaccggctgg
      901 cggagagctt cgacctgctt gagttcaagg acgggcgggc cttcgagcgc tactcgcggc
      961 cgcagctgct cgacggcgag ccggtcgggc gcgtgtggag ctttcgtgac gtcaccgagc
     1021 gccgccgcgc cgagcaggag gccgagcgca gcaagctgca agatcaggtg atcgcggccc
     1081 agcggctggt gctgtcgcag ctgtcgacgc cgctgatccc gatcagcgcc gaggtgctcg
     1141 tgatgcccct gatcggcacg atcgacgacg agcgcgcgcg ccagatcgtc gccaccctgc
     1201 tcgacggcat cgcccgcacg cgggcccgcg tcgtcatcgt cgacatcacc ggcgtcgcgg
     1261 gcgtcgacac gcaggtcgcc ggcgggctgc tgcgcgccgc ccaggccgtc aagctgctcg
     1321 gggcgcaggt cgtgctcacc ggcatccgcc cggaggtcgc gcagacgctg gtgtcgctcg
     1381 gggtcgacct cggcgggctc gtcactcgca gcacggtgca gacgggtatc gcctacgcga
     1441 ccgagcggcg cggctgaggc cgggcgtcac gcgcagttgc cggaggtcac gacctggccg
     1501 gtgacgggca cgccgtcgcc gggcacgtcc gcgaaggtga cggcgatcga ctggcaccag
     1561 ccgtacggac agttctcctg gcgggcgacg tgtgcgtgcg ggccggtcga ctcgccggcg
     1621 ccggacgag
//
LOCUS       DASWNJ010000234         1975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_39910, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000234 DASWNJ010000000
VERSION     DASWNJ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39910"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VGB85_04280"
     CDS             <1..462
                     /locus_tag="VGB85_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133267.1"
                     /translation="LAQAGVEGGLATAAAIAAGSDQAIKDFNKLQGEISSVGVNLGNA
                     TADSMYAAGIKVSEGVVRGLERQGAVIDKAAQKLARRISTAIEVEMGGKSRVKDRPKL
                     KDSATSSRTAAGAGGTVTVRLTAEQMSRVQRGKEIILDVNAARRAGVRFDS"
     gene            603..>1975
                     /locus_tag="VGB85_04285"
     CDS             603..>1975
                     /locus_tag="VGB85_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133268.1"
                     /translation="MDPVSFSTVRPGRAVRARVTDGVGGWEDIFNAKVEKCSATRNPR
                     AKSGRTVNVRLDVVDVVMTLQNLLERRGVATIDGLRWLFAGRGFAFNVNGQVTALGAQ
                     TVVAGNASANLWDQVLITRDTNLGYAWVDKAGAVRVFDRASMPAGSVATIGPDVYSSL
                     NTDFDLEQIINQVVVNWLRYDIGTEQSTPVPYGPYPDPDSVDEWGAQQVEITIQGATE
                     VEANIIDYAQDVLDLNATAEVRPRSCVIPMRSVADLALVRAIDLNSRVTVVYHDGVTA
                     KVMRVVRIKHSITTASGWIVELEFALPTGISMPSQTTGSPTSYIPDGAIHEAQLDAAL
                     QETITSAAELAAEAVADAEEALNFAESATRTFYQPTMPTEGEVGWTWFDTDDGNRIHV
                     HNGVTYVDPGLARLTDVAEQIEPFAVDLSGAVADLAAVSDLFPIESVSIADDAITAPK
                     IAAGAITT"
BASE COUNT          345 a          664 c          695 g          271 t
ORIGIN      
        1 ctcgcgcagg cgggtgtcga gggcggcctc gcgacggcgg cggcgatcgc cgccggcagt
       61 gaccaggcga tcaaggactt caacaagctg cagggcgaga tcagctcggt cggagtcaac
      121 ctcgggaatg ccacggcaga cagcatgtat gccgctggga tcaaggtctc cgagggcgtc
      181 gtccgtgggc tcgagcggca gggcgccgtg atcgacaagg cggcgcagaa gctcgcacgc
      241 aggatctcga cggcgatcga ggtcgagatg ggcgggaagt cgcgggtcaa ggatcggccg
      301 aagctgaagg actcggcgac gtcgtcgcgg actgccgcag gggcgggcgg cactgtcact
      361 gtgcggctga ccgctgagca aatgtcgcga gtgcagcgcg gtaaggagat catcctcgac
      421 gtcaacgccg cccgcagggc cggcgtgagg ttcgactcgt gatcaacccg aaccaggtcg
      481 cgcggctcga ggtccacaac ggcgtcacct gggtcaacat caccggcccc acgagctcga
      541 tcgagatcga ccgggtcgag agcagcgcaa gcacgctcag cgcggaagtc ctcgacgcga
      601 ccttggatcc ggtctcgttc tccaccgtcc ggcccggccg ggcagtccgt gcgcgggtga
      661 cggacggcgt cggcggctgg gaggacatct tcaacgccaa ggtcgagaag tgctcggcca
      721 cccgcaaccc gcgcgcgaag tccggccgca ccgtgaacgt gaggctcgac gtcgtcgacg
      781 tggtcatgac gctgcagaac ctgctcgagc gccgtggcgt ggccacgatc gacgggctcc
      841 gctggctctt cgccggcagg gggttcgcct tcaacgtcaa cggccaggtg accgcgctcg
      901 gtgcgcagac cgtggtggca ggcaacgcct cggcgaacct ctgggaccag gtgctcatca
      961 cccgcgacac gaacctgggg tacgcgtggg tcgacaaggc cggcgcggtg cgggtcttcg
     1021 accgggcgag catgcccgcg ggcagcgtgg ccaccatcgg tcccgacgtc tactcgagcc
     1081 tgaacactga cttcgacctc gagcagatca tcaaccaagt cgtcgtgaac tggctgcgct
     1141 acgacatcgg caccgagcag tcgacgcccg tgccgtacgg cccctacccc gacccggact
     1201 cggtcgacga gtggggtgcg cagcaggtcg agatcacgat ccagggcgcc accgaggtcg
     1261 aggcgaacat catcgactac gcgcaggacg tgctcgacct gaacgccacc gccgaggtgc
     1321 ggccgcggtc gtgcgtgatc ccgatgcgca gcgtcgcgga cctggcgctc gtgcgggcga
     1381 tcgacctcaa ctcccgggtg acggtggtct accacgacgg cgtcacggcg aaggtcatgc
     1441 gggtcgtccg gatcaagcac tcgatcacga cggcgagcgg ctggatcgtc gagctcgagt
     1501 tcgcgctgcc gaccggcatc agcatgccgt cgcagaccac cggctcgccg acgtcgtaca
     1561 tcccggacgg agcgatccac gaggcgcagc tcgacgcggc gctgcaggag accatcacca
     1621 gcgccgccga gctggcagcc gaagcggtcg ccgacgctga ggaagccctg aacttcgcgg
     1681 agtccgcgac gcgcacgttc taccagccca cgatgcccac tgagggcgag gtcgggtgga
     1741 cgtggttcga caccgacgac ggcaaccgga tccatgtgca caacggcgtc acgtacgtcg
     1801 accccgggct ggcgcggctg acggacgtgg ccgagcagat cgagccgttc gcggtggacc
     1861 tctcgggcgc cgtcgcggac ctggccgccg tctctgacct gttcccgatc gagtcggtca
     1921 gcatcgcgga cgacgcgatc accgcgccga agatcgccgc gggtgccatc accac
//
LOCUS       DASWNJ010000235         1667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_215856, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000235 DASWNJ010000000
VERSION     DASWNJ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215856"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1667)
                     /gene="dnaG"
                     /locus_tag="VGB85_04290"
     CDS             complement(<1..>1667)
                     /gene="dnaG"
                     /locus_tag="VGB85_04290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY0133269.1"
                     /translation="DLVDLVGRYVGLKRAGKNFAACCPFHNERTPSFYVNPERRSYKC
                     FGCGVWGTGIDFVMQMEGKSFLEACRQLAQLYGVQLPSGLKDNTSTAQQNERDEAYAI
                     NRTAAELYREILLHGDEGQLGRLYQERRGITAETAEAFQLGYAPAPAEAGWDRLTNHL
                     QRKGLSLPLAEKLGLVARSERTEKYYDKFRGRLMFPVVQPGGAVIAFSGRILPEHAAP
                     DADGREAPKYTNSPESLLYTKGKTLFGLHLAGQNMRRAGRAILVEGNVDVVTMHQRGY
                     RETVAPLGTALTAQQVTLLGRFAETCVLCFDGDSAGRKAARNAIPLLLDAGIEARIVA
                     LEGGVDPDTADPGRLASLLEQPGSALEWLMRWMVAAGARDSIDAQDRALSALAPLLAK
                     VKRDGARELYIDRAAKLLGLPPGRISSALQGSREAPRPPNHRDPNMREPSGGPAAGPA
                     GAAGPNGSAQRSGPARPLPELPRSQVALAALLVDNPHLASLAEQQRVRDFVTDPRLAP
                     IVGAVIDAAFSGDSPGVGELLDLIEVAAQRQVHDALFAGRYRDEENP"
BASE COUNT          229 a          525 c          659 g          254 t
ORIGIN      
        1 gggggttctc ttcgtcgcgg tagcgaccgg cgaacagggc gtcgtggacc tgccgctggg
       61 ccgcaacctc aataagatcg aggagctcgc ccacgcccgg actgtcaccg gagaaggcgg
      121 cgtcgatcac cgcgccgacg atcggcgcca ggcgcgggtc cgtcacgaag tcccgcaccc
      181 tttgttgctc cgcgaggctg gcaaggtgtg ggttgtccac cagcagggcc gccagtgcaa
      241 cttgactgcg cggaagctca ggcagaggcc gggcagggcc ggatctctgt gcggacccgt
      301 tcggaccggc ggcacccgcg gggccggcgg caggaccccc cgaaggctcg cgcatgttcg
      361 ggtcgcggtg gttgggcggg cggggcgctt cacggcttcc ttgcagcgcg gacgatatgc
      421 gcccgggagg cagtccaagc aatttagcgg cgcggtcgat gtagagctct cgcgcaccgt
      481 cgcgcttgac tttcgccagc aagggggcga gcgcgctgag agcgcggtcc tgggcgtcga
      541 tgctgtcgcg cgcgccggcc gcgaccatcc agcgcatcaa ccactcgagg gccgagccgg
      601 gctgctccag tagcgaggcg aggcggccgg ggtcggcggt gtcggggtcg acgcccccct
      661 cgagggcgac gatgcgggcc tcgatgccgg cgtccaacag cagcgggatg gcgttgcggg
      721 ccgccttgcg gccggcgctg tcgccgtcga agcagagcac gcaggtctcg gcgaagcggc
      781 cgagcagggt gacctgctgg gcggtgaggg ccgtgccgag cggggcgacg gtttcgcggt
      841 agccgcgttg gtgcatggtg acgacgtcga cgttgccctc gacgaggatc gcccggccgg
      901 cgcggcgcat gttctggccg gcgaggtgca ggccgaacag ggtcttgccc ttggtgtaca
      961 gcagcgactc cggcgagttg gtgtacttcg gggcctcgcg gccgtcggcg tccggggccg
     1021 cgtgctcggg caggatgcgg ccggagaagg cgatgacggc gccaccgggc tggacgaccg
     1081 ggaacatcag gcggccgcgg aacttgtcgt agtacttctc ggtgcgctcg gagcgggcga
     1141 cgaggccgag cttctcggcg agcgggaggg acaggccctt gcgctggagg tggttggtga
     1201 ggcggtccca gccggcctcg gcgggggcgg gcgcgtagcc gagctggaac gcctcggcgg
     1261 tctcggcggt gatgccccgg cgctcctggt acaggcggcc gagctggccc tcgtcgccgt
     1321 gcagcaggat ctcgcggtag agctcggcgg cggtgcggtt gatcgcatag gcctcgtcgc
     1381 gctcgttctg ctgggcggtc gaggtgttgt ccttgaggcc ggacggcagc tgcacgccgt
     1441 agagctgcgc gagctggcgg caggcttcca gaaaactctt gccctccatc tgcatcacga
     1501 agtcgatgcc ggtgccccac accccgcagc cgaagcactt gtagctgcgg cgctcggggt
     1561 tgacatagaa gctcggggtg cgctcattgt ggaaggggca gcaggcggcg aagttcttgc
     1621 ccgcgcgctt gagcccgacg tagcggccga cgagatcgac gagatcg
//
LOCUS       DASWNJ010000236         1981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_534794, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000236 DASWNJ010000000
VERSION     DASWNJ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534794"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VGB85_04295"
     CDS             <1..418
                     /locus_tag="VGB85_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133270.1"
                     /translation="RSGGYSLYVSGQATRDDVAVRFAWGFTTTTRYTACESQALVDNL
                     HAPKIELTIHGDHLFYDDLFASEPKVSFDLIASADADGDGEVTQAELTAVDLRPLANY
                     QVGSTGIVDLWHFIEHQTTTLGHIDGEGHCEATRES"
     gene            complement(478..948)
                     /locus_tag="VGB85_04300"
     CDS             complement(478..948)
                     /locus_tag="VGB85_04300"
                     /inference="COORDINATES: protein motif:HMM:NF021438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HEY0133271.1"
                     /translation="MHATIVSLFKHPAKLAATAFIATLLFVASSVMAFSGRTTAVGYW
                     KTIDDETGDAKSIVKIYQVEDKLYGRIDRLLQDPNILCDKCEGEDHNKPVQGMVIMWG
                     MTADGDGEWSGGKIFDPSKGKTYRCSIWIKDGKLQVRGHLGPFYRTQTWLPHGD"
     gene            1137..>1981
                     /locus_tag="VGB85_04305"
     CDS             1137..>1981
                     /locus_tag="VGB85_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133272.1"
                     /translation="MGPEIFIVGDRSHRGALIPRVQDLGYTVTPVRERELSGRVASSP
                     APAAVLVCLGETNASELVDAVRRGRDDVPVVLLGSLGGELRDLADVLELGADHFLAAP
                     VGDEELARVLAELAGPGAPTVIEAEEGAGAEAGDLEDEDELDEEDEPVRPLTGRLRGR
                     SPVPGQLHRSLEMLAARLQTHESGDAEPDNLDLESLGLDATPDVDGELDPQVGPLLTD
                     PESSDPAAPQRPEPTQRISARPRPVTDHEDSRGAMQGEDTQQLQLRDEAEAGHVRRDM
                     EELAAP"
BASE COUNT          306 a          687 c          699 g          289 t
ORIGIN      
        1 tcgcagcggc ggttattcgc tgtacgtgtc cgggcaggcg acgcgtgacg atgtcgcggt
       61 gcgcttcgcg tggggcttca ccacgaccac gcgctacacc gcgtgcgagt cgcaggcgct
      121 cgtcgacaac ctccacgccc cgaagatcga gctgacgatc cacggcgacc atctgttcta
      181 cgacgacctg ttcgcgtccg agccgaaggt cagcttcgac ctgatcgcct cggccgacgc
      241 ggacggcgac ggtgaggtca cgcaggcgga gctgaccgcg gtcgacctgc ggccgctggc
      301 gaactatcag gtcggcagca ccggcatcgt cgacctgtgg cacttcatcg agcaccagac
      361 gaccacgctc gggcacatcg acggcgaggg ccactgcgag gccacgcgcg agtcctgaac
      421 gcttaaacgc gaacagcgac ccgcgggggt cgctgtcacg gtgtcacggg ggacttctca
      481 gtcgccgtgg ggcagccagg tctgggtgcg gtagaacggg cccaggtggc cgcggacctg
      541 cagcttgccg tccttgatcc agatcgagca gcgataggtc ttgcccttgg acgggtcgaa
      601 gatcttgccg ccgctccact ccccgtcgcc gtccgccgtc atgccccaca tgatgaccat
      661 gccctgcacg ggcttgttgt ggtcctcgcc ctcgcacttg tcacacagga tgttcgggtc
      721 ttgcagcagg cggtcgatac gaccgtagag cttgtcctcg acctggtaga tcttcacgat
      781 cgacttggcg tcgccggtct cgtcgtcgat cgtcttccag tagccgaccg cggtggtccg
      841 accggagaac gccatcacgg aggaggcgac gaacagcagg gtggcgatga aggcggtggc
      901 cgcgagcttg gccgggtgct tgaacagcga gacgatggtt gcgtgcatcg gtgagatcct
      961 cagggcaggg aaggcaggag ggcccagtgt accgcgaccc gaacgaggac gggcaggacc
     1021 ggacaggacc ttgcatcgac gcccacctcg ccggtctatg cacgcctcga cgatcttttt
     1081 tccatgtggc cgggagcccg cggacgcctc ccgccctgag gtacactccc gccgccgtgg
     1141 gcccggagat cttcatcgtc ggcgatcgca gtcatcgtgg ggccctcatc ccccgggtgc
     1201 aggacctcgg ctataccgtg acgcccgtgc gcgagcgcga gctcagcggc cgggtcgcct
     1261 cgtcccccgc gcccgcggcc gtgctcgtct gcctcggcga gaccaacgcc tccgagctcg
     1321 tggacgccgt tcgccgcggt cgagacgatg ttccagtggt gctgctcggt agcctcggcg
     1381 gcgagctgcg cgacctcgcc gatgtgctcg agctcggcgc ggaccacttc ctcgccgcgc
     1441 ccgtcggcga cgaggagctg gcccgcgtgc tcgccgagct cgccgggccc ggcgccccca
     1501 ccgtcatcga ggcggaggag ggcgcgggcg ccgaggccgg ggacctggag gacgaggacg
     1561 agctcgacga ggaggacgaa cctgtgcggc cgctcacagg tcgcctgcgg ggccggagcc
     1621 cggtgccggg gcagctgcac cgctcgctcg agatgctcgc ggcgaggtta cagacccacg
     1681 agtctggcga cgccgagccg gacaacctcg acctcgagtc gctggggctc gacgcgacgc
     1741 cggatgtcga cggcgagctc gacccgcagg tcgggccgct gctgaccgac cccgagtcga
     1801 gcgacccggc ggcgccgcag cgccccgagc cgacccagcg gatctccgcg cgaccgcggc
     1861 cggtgaccga ccacgaggac agccgcggcg cgatgcaggg cgaggacacc cagcagctgc
     1921 agctgcgcga cgaggccgag gcgggacatg tccgaaggga catggaggag ctcgcggcgc
     1981 c
//
LOCUS       DASWNJ010000237         3004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_498545, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000237 DASWNJ010000000
VERSION     DASWNJ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498545"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="VGB85_04310"
     CDS             complement(<1..934)
                     /locus_tag="VGB85_04310"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0133273.1"
                     /translation="MLTVVVHEKGGQTQRFSFKGDSFSIGREDDNDLVLDRVNVSKHH
                     LRFVKTEGRIEVHDLESTNGTYLNGRKVANPRPIRRTDRVYVGDYILLLEGDDALLTA
                     PPRDPRRGANPVTRPGDKPEFVELVADDTGLVTSAQRVAAAGVESSYLDGLAAQIHQT
                     LLKAVPRLDPLGSNDVTDDDRKDVIEMVDGVLADFRDSQQIEATVDVGPFRERVLREA
                     LEWGPLGDLMRDDSVREIQVVGTSPLHVVRDSGVAERLELRFTGEAALMRVIQRMTRK
                     RGLMGEGTHVLEGTVEHGFYLYALLPPHPGRQIVV"
     gene            complement(1078..1659)
                     /locus_tag="VGB85_04315"
     CDS             complement(1078..1659)
                     /locus_tag="VGB85_04315"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HEY0133274.1"
                     /translation="MSNAPGGSPPPADGPVGHSPLGTPSGNPEQAEALATELSHDLMS
                     PYCPGRTIATCPSPNARKLEALILEQAQAGKSRLEIETALVQQFPDIRGYVGRPEIIY
                     GTALVALIAIVGLVLAGRRWVRHGRQPAAMAAMSGAGAGAGVAAAAVSPESPASKVPT
                     EPVVPPSAVAAPGEASRRELDALDDALDRIDEF"
     gene            complement(1760..2572)
                     /locus_tag="VGB85_04320"
     CDS             complement(1760..2572)
                     /locus_tag="VGB85_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133275.1"
                     /translation="MTGALRRDLGCLCGRALALVLLLPGLLAGCGGGDGKTLAEKMGG
                     DPEKEALEKAGYKANPKPRPTGLPPPTDAEFSAWDRKDPEGERHLHKFDKANLKKLLN
                     FWAELDCFREKMKMEGTKAVGVEPGSPAGEQWHQFKQIFIPMVNAWQQRMFASEPRIL
                     EKSKLIGHFLEAHELVMNGYPDAYNNSDQLAIATADAQWTIVENKIIKYLKNITSEEL
                     PKEDLTDPTVKEKHDKFCMAALNPPKNTGKAKIKHVGGGGSRGPKKLNMGDD"
     gene            2768..>3004
                     /locus_tag="VGB85_04325"
     CDS             2768..>3004
                     /locus_tag="VGB85_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133276.1"
                     /translation="MPPRSPAPSPAPAKTANQSANKSASKSAKAPTTTARAPAKPAKP
                     AVTGPSGQEPKASPANRLKVGDRAPEFALPADDGR"
BASE COUNT          385 a         1011 c         1124 g          484 t
ORIGIN      
        1 cgacgacgat ctgtcggccc gggtgcggcg gcagcagggc gtacaggtaa aagccgtgct
       61 cgacggtgcc ctcgagcacg tgggtgccct cgcccatcag gccgcgcttg cgggtcatcc
      121 gctggatgac gcgcatcagg gcggcctcgc cggtgaagcg cagctcgagg cgctcggcga
      181 cgccgctgtc gcggacgacg tgcagcgggc tggtgccgac gacctggatc tcgcggacgc
      241 tgtcgtcgcg catcaggtcg cccagcgggc cccactcgag cgcctctcgc agcacgcgct
      301 cgcggaacgg gccgacgtcg acggtcgctt cgatctgctg cgagtcgcgg aagtcggcga
      361 gcacgccgtc gaccatctcg atgacgtcct tgcggtcgtc atcggtgacg tcgttgctgc
      421 cgagcgggtc gaggcgcggc accgccttga gcagggtctg gtggatctgc gcggccaggc
      481 cgtcgaggta gctgctctcg acgccggcgg cggcgacccg ctgggccgag gtgacgagcc
      541 cggtgtcgtc ggcgaccagc tcgacgaact cgggcttgtc gccgggtcgg gtgaccgggt
      601 tggcgccgcg tcgggggtcg cgtggcggcg ctgtcagcag cgcgtcgtcc ccctcgagca
      661 gcaggatgta gtcgccgacg tagacgcggt cggtgcggcg gatcggcctc gggttggcga
      721 ccttgcgacc gttgaggtac gtaccgttgg tgctctcgag gtcgtggacc tcgatccggc
      781 cctcggtctt gacgaagcgc aggtggtgct tcgagacgtt gacgcggtcg aggacgaggt
      841 cgttgtcgtc ctcgcggccg atcgagaagc tgtcgccctt gaaggaaaaa cgctgggttt
      901 gcccgccctt ctcgtggact actacggtca gcacacgcca ggcttacccc gaagggacac
      961 gccgactcaa gggccggcgc tcgcgcatcg cgcgcgaaag tccgcgcgaa gccgcgtgta
     1021 gatcccgccg cggacgcggt tcgccgcggt cgccagcgcg atcgtcgtcg cgggcgccta
     1081 gaactcgtcg atgcggtcga gcgcgtcgtc gagggcgtcg agctcgcggc gcgaggcctc
     1141 gcctggcgcg gcgactgccg atggcgggac caccggctcg gtcggcacct tggacgcggg
     1201 tgactccggg gacaccgccg cggctgcgac ccctgcccct gcccctgccc ctgacatggc
     1261 ggccatggcg gccggctggc ggccatggcg gacccagcga cggccggcga gcacgaggcc
     1321 gacgatcgcg atcagggcga cgagggcggt cccgtaaatg atctccgggc ggccgacgta
     1381 gccgcggatg tccgggaact gctggacgag cgccgtctcg atctcgaggc gggacttgcc
     1441 ggcctgggcc tgctcgagga tcagggcctc gagcttgcgg gcgttgggcg aggggcaggt
     1501 ggcgatcgtg cggccggggc agtacggcga catcaggtcg tgcgacagct cggtcgccag
     1561 cgcctcggcc tgctccgggt tcccggacgg ggtgcccagc ggcgagtggc cgaccgggcc
     1621 gtccgcgggc ggcgggctgc cgccgggggc gttgctcacg gggtcgctcg cgggcgcggg
     1681 cgcctgggcg ttcgcggggg ccgcgccagg ggctcccgca gaggcgggag cggcaggctg
     1741 cgcgatctct tccatcggct cagtcgtcgc ccatgttgag cttcttgggg ccccggctgc
     1801 cgccgccgcc gacgtgcttg atcttcgcct tgccggtgtt cttgggcggg ttgagggccg
     1861 ccatgcagaa cttgtcgtgc ttctccttca ccgtcgggtc ggtgaggtcc tccttgggca
     1921 gctcctccga ggtgatgttc ttgaggtact tgatgatctt gttctcgacg atcgtccact
     1981 gggcgtcggc ggtggcgatc gccagctggt cgctgttgtt gtaggcgtcg gggtagccgt
     2041 tcatcaccag ctcgtgggcc tcgaggaagt ggccgatcag cttggacttc tcgaggatcc
     2101 gcggctcgct ggcgaacatg cgctgctgcc aggcgttcac catcgggatg aagatctgct
     2161 tgaactgatg ccactgctcg ccggccgggc tgcccggctc gacgccgacc gccttggtgc
     2221 cctccatctt catcttctcg cggaagcagt cgagctcggc ccagaagttg agcaacttct
     2281 tgaggttggc cttgtcgaac ttgtggaggt ggcgctcgcc ctcggggtcc ttgcggtccc
     2341 aggcggagaa ctccgcgtcg gtcggcggcg gcagacccgt cgggcgcggc ttggggttgg
     2401 ccttgtagcc ggccttctcg agcgcctcct tctcggggtc gccgcccatc ttctcggcca
     2461 gggtcttgcc gtcgccgccg ccgcagcccg cgaggaggcc aggaagcagc agcacaagcg
     2521 ccagcgcgcg cccgcacagg cagccgagat cacgtcgcaa cgcaccggtc atgtcgtgtg
     2581 tcctgtcgtc cagaggagcc cgactttagc cgggccgcac cgcgcgtgta aagcaacgcc
     2641 gcccggcgat gccgcgcgga ggccgcggcc ggatctcgcg gggccgctgc gggtggcgtg
     2701 ggcgagcttg cgagattgcc cgtgggtggt ggtgcggggt agaaggtcgc ctcccggaaa
     2761 agcacgcatg ccgccccgaa gccccgcgcc ctcccctgcc cctgccaaga cggcgaacca
     2821 gtcggcgaac aagtcggcga gcaagtcggc gaaggccccg accacgaccg cccgcgcgcc
     2881 tgcgaagccc gcgaagccgg ccgtcaccgg tccttcggga caggaaccca aggccagccc
     2941 ggcgaacagg ctcaaggtcg gcgatcgggc cccggagttc gcgctgccgg ccgacgacgg
     3001 gcgg
//
LOCUS       DASWNJ010000238         3252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_675149, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000238 DASWNJ010000000
VERSION     DASWNJ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675149"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VGB85_04330"
     CDS             complement(<1..640)
                     /locus_tag="VGB85_04330"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEY0133277.1"
                     /translation="MTPSRLRPRALRPGARVHVVAPSGPVPSDRLEAGLAALRDQVLP
                     PHITGEISLADNVGARAGYFAGDDPTRLAALQAALADPEIDAIVCARGGYGLTRLIGS
                     LDPARLRAAPKLIVGFSDVTALLAWAHVGAGLVSVHGPVVTQLACLAEEDRERLGLML
                     IGEDPGALVAAAGAVLRGGCVEGPLITANLEMLRSLVGTKFMPDLAGCILALE"
     gene            800..>3252
                     /gene="secA"
                     /locus_tag="VGB85_04335"
     CDS             800..>3252
                     /gene="secA"
                     /locus_tag="VGB85_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY0133278.1"
                     /translation="MLNAFIKKVFGTKHERQMKRMQPLIDRINALEPKMKGLADADFP
                     GITADLRARLAKGQPLDELTAEAFAAVREASVRALGMRHYDVQMIGGIALHQGRIAEM
                     RTGEGKTLTATTALYLNALGGRGAHLITVNDYLARRDAEWMGQCYRFLGMSCGVIVGG
                     MYHTERQKNYRADITYGTNNEFGFDYLRDNMKETIEKYVQRDLNYAIVDEVDSILIDE
                     ARTPLIISGEANKPPDTYVTVNRVIPSLRRDLDFVVDEKAHSVQLTDGGVDRIEKLLS
                     CRNLYAPENNELLHHVNQALRAHVLYKKDVNYIVEGGEIVIIDEFRGRKMEGRRWSDG
                     LHQAIEAKEGVEIQPESQTLATITYQNYFRMYDKLAGMTGTADTEAEEFNKIYNLDVV
                     VIPPNRKMVRQDLDDLVYKNERGKFRAVVEEIKQAHERGQPVLVGTASVEKSQVIHTM
                     LDRMGIPHEVLNAKQHEREAYIVAQAGRKGAVTVATNMAGRGTDILLGGNPDMVARSQ
                     IDPDKEPARFEALHAALKSEFEKEKAEVKGLGGLYIVGTERHESRRIDNQLRGRAGRQ
                     GDEGRSRFFLSLEDDLMRIFGADRITSLMERLGMEEDVPIEAPLVNRAIANAQSRVEA
                     MHFDTRKNLFEYDNVMNDQRKAIYALRKQILEGRYQPEILDDQARKEQSRNLPPPPEK
                     SGQHTVESLRRTVTEKITGIIDAHCRALLEEAGLPADGPEPFPPGGANPEKLAHELYR
                     HYGALINLEDVKNDRRKIIAHAIDVVARSLIQQRERVHDLTFKLVEEQVLKLCPETVH
                     PDEWDLEALDQVLQERFKV"
BASE COUNT          614 a         1135 c         1095 g          408 t
ORIGIN      
        1 cctcgagcgc gaggatgcag ccggcgaggt cgggcatgaa cttcgtgccg acgaggctgc
       61 gtagcatctc gaggttcgca gtaataagag ggccctcgac gcagccgccg cgcagcacgg
      121 cgccggccgc ggcgacgagc gccccggggt cctcgccgat gagcatcagg ccgaggcgct
      181 cgcggtcctc ctcggcgagg caggcgagct gcgtgacgac gggcccgtgc acgctgacga
      241 ggccggcgcc gacgtgggcc caggcgagca gcgcggtgac atcggagaag ccgactatca
      301 gcttcggcgc ggcgcgcagg cgggcggggt cgagcgagcc gatcagtcgg gtcaggccgt
      361 agccgccgcg agcgcagacg atggcgtcga tctccgggtc ggcgagcgcg gcctgcaagg
      421 cggccaggcg ggtcgggtcg tcgcccgcga agtagccggc gcgcgccccg acgttgtctg
      481 cgagcgagat ctcacccgtg atgtgcggcg ggagaacctg gtcgcgcagg gcagccaggc
      541 cggcttcgag gcgatccgag gggaccgggc ccgaaggggc gacgacgtgg acgcgggcgc
      601 ccgggcggag ggcgcgcggg cgcaggcggg agggcgtcac gggggcggac gatagcagcg
      661 gcggagggcg tcagaaaacc ccggtcgtcg tgccatcttc cggcgtggac acgggcggat
      721 cccgcccgta cccttggccc ccgcgtgggc cagccctggt ctacgggtcg gccacgccca
      781 gaaactgccc tttatcgcca tgctcaacgc gttcatcaag aaagttttcg gcaccaagca
      841 cgagcggcag atgaagcgca tgcagccgct gatcgaccgg atcaacgcgc tcgagccgaa
      901 gatgaagggc ctcgccgacg ccgacttccc cggcatcacc gccgacctgc gcgcccgcct
      961 ggcgaagggc cagccgctcg acgagctgac cgccgaggcc ttcgccgccg tgcgagaagc
     1021 ctccgtacgc gcgctcggca tgcgccacta cgacgtgcag atgatcggcg gcatcgccct
     1081 gcatcagggc cgcatcgccg agatgcgcac cggtgagggc aagacgctga ccgcgacgac
     1141 ggcgctgtac ctcaacgcgc tcggcggccg cggggcccac ctgatcaccg tcaacgacta
     1201 cctggcccgc cgcgacgccg agtggatggg ccagtgctac cgcttcctcg gcatgtcgtg
     1261 cggcgtgatc gtcggcggca tgtatcacac ggagcggcag aagaactacc gcgccgacat
     1321 cacgtacggc acgaacaacg agttcggctt cgattatctc cgcgacaaca tgaaggagac
     1381 gatcgagaag tacgtgcagc gcgacctcaa ctacgcgatc gtcgacgagg tcgactcgat
     1441 cctgatcgac gaggcccgca cgcccttgat catctccggc gaggccaaca agcccccgga
     1501 cacctacgtc accgtcaacc gcgtgatccc ctcgctgcgc cgcgacctcg acttcgtcgt
     1561 cgacgagaag gcccacagcg tccagctgac cgacggcggc gtcgaccgca tcgagaagct
     1621 gctcagctgc cgcaacctct acgcgccgga gaacaacgag ctcctgcacc acgtcaacca
     1681 ggcgctgcgg gcccacgttc tctacaaaaa ggacgtcaac tatatcgtcg agggcggcga
     1741 gatcgtcatc atcgacgagt tccgcggccg caagatggag ggccgccgct ggtccgacgg
     1801 cctgcaccag gcgatcgagg ccaaggaggg cgtggagatc cagccggaga gccagaccct
     1861 cgccacgatc acgtaccaga actactttcg catgtacgac aagctggccg gcatgaccgg
     1921 cacggccgac accgaggcgg aggaattcaa taagatctac aacctcgacg tcgtcgtcat
     1981 cccgccgaac cgcaagatgg tccgccagga cctcgacgac ctcgtctaca agaacgagcg
     2041 cggcaagttc cgcgcggtgg tcgaggagat caagcaggcc catgagcgcg gccagccggt
     2101 gctcgtcggc accgcgagcg tcgagaagtc gcaggtgatc cacacgatgc tcgaccgcat
     2161 gggcatcccc cacgaggtcc tcaacgccaa gcagcacgag cgcgaggcgt acatcgtcgc
     2221 ccaggccggt cgcaagggcg ccgtgacggt cgccaccaac atggccggcc gcggcaccga
     2281 catcttgctc ggcggcaacc cggacatggt cgcccgctcg cagatcgacc ccgacaagga
     2341 gccggcgcgc ttcgaggcgc tgcacgccgc gctcaagtcc gagttcgaga aggagaaggc
     2401 ggaggtcaag ggcctcggcg gtctctacat cgtcggcacc gagcgtcacg agagccgccg
     2461 catcgacaac cagctgcgcg gccgcgccgg ccgtcagggc gacgagggcc gctcgcgctt
     2521 cttcctctcg ctcgaggacg acctgatgcg catcttcggg gccgaccgca tcaccagcct
     2581 gatggagcgc ctcggcatgg aggaggacgt cccgatcgaa gcgccgctgg tcaaccgggc
     2641 gatcgccaac gcccagtcgc gcgtcgaggc gatgcacttc gacacccgca agaacctgtt
     2701 cgagtacgac aacgtgatga acgaccagcg caaggcgatc tacgccctgc gcaagcagat
     2761 cctcgagggc cgctatcagc cggagatcct cgacgaccag gcgcgcaagg agcagtcgag
     2821 gaacctgccg ccgccgcccg agaagtcggg ccagcacacg gtcgagtcgc tgaggcgcac
     2881 ggtgaccgag aagatcaccg gcatcatcga cgcccactgc cgcgcgctgc tcgaggaggc
     2941 cggtctgccg gccgacggcc ccgagccctt cccgcccggc ggcgccaacc cggagaagct
     3001 cgcccacgag ctgtaccgcc actacggcgc gctcattaac ctcgaggacg tcaagaacga
     3061 ccgccgcaag atcatcgcgc acgcgatcga cgtcgtcgcg cgctcgctga tccagcagcg
     3121 cgagcgcgtg cacgacctta ccttcaagct cgtcgaggag caggtcctca agctgtgccc
     3181 ggagacggtg cacccggacg agtgggacct cgaggccctc gaccaggtgc tgcaggagcg
     3241 cttcaaggtc aa
//
LOCUS       DASWNJ010000239         1845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_40041, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000239 DASWNJ010000000
VERSION     DASWNJ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40041"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VGB85_04340"
     CDS             <1..930
                     /locus_tag="VGB85_04340"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEY0133279.1"
                     /translation="VVMPAGPMFPVGTGAPEPTPIIGGEAVEGAEWASVVAVLSVDPV
                     DSSVGHLCTGTLIAPQIVLTAAHCLAPGTELEEIGVFFGTSMTHQQMATVVRYGLYPG
                     ACVKDCKKDAFDFAFIEIREKVGGVAIIPLLTTQEEWDEAMAPDMPVTVVGFGAVRDE
                     EQEGEPPLTDKERGFKRVVATVIDEIRPGGREFVAGEQGKDTCGGDSGGPAFVQLADG
                     SWRQVGVTSRGVRPCGTGRGYYGVPFFALPWLRDKAGLDLLPADCPEADCIDTSPPEE
                     GCGCRGGGTGGLAGLAGLAGLLSLLRRRRRVSS"
     gene            complement(920..>1845)
                     /locus_tag="VGB85_04345"
     CDS             complement(920..>1845)
                     /locus_tag="VGB85_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133280.1"
                     /translation="SVGLLPPVLVAAALLASRHVPKDMSQWPGSFISLLCLVLPALMW
                     LQAGVRVEAIFGLGPAAVGVLALLFALLAPLWPTRPRPLLLALTLSLLAGAALTLATP
                     AFSEDRPRRLSLVFHQDADTSSARWLIDGDLALPRILKTVAEFTSAPAFAWTPADEPS
                     WISPTAAIAAPPPELLPVPDTPSISGPWGRYLRLRLRSPRGARSAALVIPDDAGLRWL
                     AIASETVPPYPAHRRDWYVDVRHHPIVALPPEGVLVELVLRSPDPVTFTVLDVHEDLP
                     PSAAQLLDARPAWAIPSHGGDRSVHSRSFSY"
BASE COUNT          279 a          553 c          765 g          248 t
ORIGIN      
        1 gtggtgatgc cggcagggcc gatgttcccg gtcggcacgg gcgctccgga gccgacgccg
       61 atcatcggcg gcgaggcggt cgagggggcg gagtgggcga gcgtggtcgc ggtgctcagc
      121 gtcgatccgg tcgactcgtc ggtcggtcat ctgtgtaccg gcacgctgat cgcgccgcag
      181 atcgtgttga ccgccgcgca ctgccttgcg ccggggaccg agctcgaaga gatcggcgtg
      241 ttcttcggga cctcgatgac gcaccagcag atggccacag tggtccgcta cgggctctac
      301 cccggcgcct gcgtcaagga ctgcaagaag gacgcgttcg acttcgcctt catcgagatc
      361 cgcgagaagg tcggcggggt ggcgatcatc ccgctgctga cgacgcagga ggagtgggac
      421 gaggcgatgg cgccggacat gccggtcacg gtggtcggct tcggcgcggt ccgtgacgag
      481 gaacaggaag gcgagccgcc gctgacggac aaggagcggg gcttcaagcg cgtcgtcgcg
      541 acggtcatcg acgagatcag gccgggcggt cgcgagttcg tcgccggtga gcaaggcaaa
      601 gatacctgcg gcggcgactc cggcggcccg gcgttcgtgc agctcgccga tggctcgtgg
      661 cggcaagtcg gcgtcacgtc gcgcggggtc cgaccctgcg gcaccgggcg cggctactat
      721 ggcgtgccgt tcttcgcgct gccgtggctg cgcgacaagg ccggcctcga cctgctgccc
      781 gcggactgcc cggaggcgga ttgcatcgat acttcgccgc cggaggaggg ctgcggctgt
      841 cgtggcggtg ggaccggcgg gctcgcgggg ctcgcggggc tcgcggggtt gttgtcgctg
      901 ttgcgccggc ggcgtcgcgt cagtagctaa agctgcggct gtgcacgctg cggtcgccgc
      961 catgagaggg gatcgcccag gcgggtcgcg cgtcgagcag ctgcgcagcg gagggaggaa
     1021 gatcctcgtg gacgtcgagc acggtgaaag tgacggggtc agggctgcgc agcacgagct
     1081 cgacgagcac gccctcgggc gggagcgcga cgatcgggtg gtggcggacg tcgacgtacc
     1141 agtcgcggcg gtgcgcggga tagggcggga cggtctcgct ggcgatcgca agccagcgca
     1201 gcccggcgtc gtcggggatc acgagcgccg cgctgcgagc accacgcggt gatcgcaggc
     1261 gcaggcgcag ataacgaccc cacgggcccg agatggatgg ggtgtcgggc accgggagca
     1321 gctccggtgg cggggcggcg atcgctgcgg tcggggagat ccagctcggc tcgtccgcag
     1381 gggtccaggc gaaggcgggg gcgctcgtga attcggcgac agtctttaaa attcgcggga
     1441 gggcgaggtc gccgtcgatc agccagcggg cgctgctggt gtcggcgtcc tgatgaaaga
     1501 cgaggctgag tcgacgcggg cggtcctcgc tgaaggccgg agtggcgagc gtgagcgcgg
     1561 cgccggcgag cagtgacagg gtgagggcga gcagcagcgg gcgcgggcgc gtcggccaca
     1621 ggggggcgag cagagcgaac aggagggcga gcacgccgac ggcggcgggc ccgaggccga
     1681 agatcgcctc gacgcggacg ccggcctgta gccacatcag ggccgggagc accaggcaca
     1741 gcagggagat gaacgagcct ggccattgcg acatgtcctt tgggacatgt cgtgaggcga
     1801 gcagcgcggc ggcgacgagc accggcggga gcaggccgac gctcg
//
LOCUS       DASWNJ010000240         2411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_252385, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000240 DASWNJ010000000
VERSION     DASWNJ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252385"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VGB85_04350"
     CDS             <1..993
                     /locus_tag="VGB85_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133281.1"
                     /translation="IYGVGVLLYELLTGTCPFSSGKQTTVLAMQLLDTAIPPRRVAPE
                     ANIPGAVERVIMRALSKDPEDRFHSVADMRAALLDDTRVHKTVPPADSSRTRRTLSRS
                     VAVVPAKARGGVGMTIALALVAAGLIGWVLAGTPGLGEPDPVPVLAPPPVVAPPTPVT
                     VIPTPTRPDPEPEPHIPTPEDVVEPAPTTASTTPAPTPEPAAAVETPTKKPAGKKKSG
                     DPATKEPVNFDFEDEPDAGTEVSPAQIDEALRPLRPAAAECGKNNGVPLGTDIGVKML
                     LASDGKLTNVRPTDRPVTDPAVQCIVELIKGTKIAPAAGGKFTSAFFDYSFKVY"
     gene            1146..1901
                     /locus_tag="VGB85_04355"
     CDS             1146..1901
                     /locus_tag="VGB85_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133282.1"
                     /translation="MLRPALIATLLTLTFIPACDDRPDDAGDTEAVDSESEGAEDGDS
                     ETGAEAGESSGESSGEPPTTGEVPEEEPEGGEESVTPLVLAFAGEAVHFNTAVTASFD
                     LTGTMSVITDWPTSTTPWLAIDRNGNGKIDDGGELFGSATLLASGETAPNGFVALADL
                     DSNHDGRVDATDEQWSRLLVWSDVDGDRKSTVNELKPVAARDLVAVELDYTVDRRCDV
                     RGNCEVERAAFIYRDGRGQELHGAVIDVYLRLR"
     gene            1959..>2411
                     /locus_tag="VGB85_04360"
     CDS             1959..>2411
                     /locus_tag="VGB85_04360"
                     /inference="COORDINATES: protein motif:HMM:NF013263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEY0133283.1"
                     /translation="MSPEQFLDAIFRHRAIAIFRGHHQGRAAAAMDAAVSAGFRLVEF
                     TVTTPGVLELIAEFSRRPGVIVGAGTVLDIAALEAVVGAGAKYLASPVLDPHILSAAR
                     DLDVAVLPGCFSPTELLQAHRSGAACQKLFPAPGYLPSYIRAVLAPMPF"
BASE COUNT          370 a          885 c          843 g          313 t
ORIGIN      
        1 atctacggcg tcggcgtgct gctttatgaa ttgctgaccg gcacctgccc gttctccagc
       61 ggcaagcaga cgacggtgct ggcgatgcag ctgctcgaca ctgcgatccc tccgcggcgg
      121 gtcgcacccg aggccaacat ccccggcgcg gtcgagcgcg tcatcatgcg cgcgctcagc
      181 aaggacccgg aggaccggtt ccacagcgtc gccgacatgc gcgccgcgct gctcgacgac
      241 acgcgggtgc acaagaccgt gccgccggcg gactcgagcc gcacgcgcag gacgctgtcg
      301 cgcagcgtcg ccgtggtgcc cgcgaaggcg cgcggcggcg tcggcatgac gatcgcgctg
      361 gcgctggtcg ccgcggggct gatcggctgg gtgctcgccg gcacgcccgg gctcggcgag
      421 cccgacccgg tgcccgtact cgcgccgccg ccggtggtcg ccccgccgac gcccgtcacg
      481 gtgatcccga cgccgacgcg cccggacccg gagcccgagc cgcacatccc gacgccggaa
      541 gacgtcgtcg agccggcgcc gacgaccgcg agcacgacgc cagcgccgac gccggagccc
      601 gccgcagcgg tcgagacgcc gacgaagaag cccgcgggca agaagaagtc gggcgatccc
      661 gcgacgaagg agccggtcaa cttcgacttc gaggacgagc ccgacgcggg caccgaggtc
      721 tcgccggcgc agatcgacga ggcgctgcgt ccgctgcgtc cggcggccgc ggagtgcggc
      781 aagaacaacg gcgtgccgct cggcacggac atcggcgtaa agatgctgct cgcgtcggac
      841 ggcaagctga ccaacgtgcg cccgaccgac cgcccggtga cggaccccgc ggtgcagtgc
      901 atcgtcgagc tgatcaaagg cacgaagatc gcaccggccg cgggcggcaa gttcacgagc
      961 gcgttcttcg actacagctt caaggtgtac tgacacccgc gccgtcatgg tgccgctgac
     1021 aacgctgtcg cggtcagcac gcaacggtgg cccgccggag gacccgaatt gccgccagag
     1081 cccgccagga gccacgcagg gccgttggca ccggtcatgc acaagctgtc ggtgaggacc
     1141 ccgccatgct ccgccccgcc ctgatcgcca ccctgctgac cctgaccttc atccccgcct
     1201 gcgacgatcg cccggacgac gcgggggaca cggaggccgt cgacagcgag tccgagggcg
     1261 ccgaggacgg cgacagcgag accggagccg aggccggcga gtcgagcggc gagtcgagcg
     1321 gcgagccccc gacgaccggc gaggtgcccg aggaggagcc cgagggcggt gaggagagcg
     1381 tgacgccgct ggtgctcgcg ttcgcgggcg aggcggtgca cttcaacacg gcggtcacgg
     1441 cgagcttcga cctgaccggc acgatgtcgg tgatcaccga ctggccgacc tcgacgaccc
     1501 cctggctggc gatcgaccgc aacggcaacg gcaagatcga cgacggcggc gagctgttcg
     1561 gctcggcgac gctgctggcc tccggcgaga cggcacccaa cggcttcgtc gcgctcgccg
     1621 acctcgactc gaaccacgac ggccgcgtcg acgcgaccga cgagcagtgg agccgcttgc
     1681 tggtgtggag cgacgtcgac ggcgaccgca agtcgaccgt caacgagctc aagccggtgg
     1741 ccgcgcgcga cctggtggcg gtcgagctcg actacacggt cgaccgccgc tgtgatgttc
     1801 gcggcaactg cgaggtcgag cgcgcggcct tcatctaccg cgacggccgc ggccaggagc
     1861 tccacggcgc cgtcatcgac gtgtatctgc gcctgcgttg agacctgtgt gcacgtgggc
     1921 acgtgacttt cccccctaac tttgggtaaa ctgtgtccat gtcgccggag caattcctcg
     1981 acgccatctt ccgccaccgg gcgatcgcca tctttcgcgg gcaccaccag gggcgcgccg
     2041 ccgcggcgat ggacgctgca gtgagcgccg gctttcgcct cgtcgagttc acggtcacca
     2101 cgccaggagt gctcgagctg atcgccgagt tctcgcggcg cccgggcgtc atcgtcggcg
     2161 ccggcacggt gctcgacatc gcggcgctcg aggcggtcgt cggcgccggc gcgaagtatc
     2221 tcgcctcgcc ggtgctcgac ccgcacatcc tgtcggccgc gcgtgacctc gacgtcgcgg
     2281 tgctgcccgg ctgcttctca ccgaccgagc tgctgcaagc ccaccgcagc ggcgccgcct
     2341 gccagaagct gttcccggcg cccggctacc tgccgagcta catccgcgcc gtgctcgcgc
     2401 cgatgccgtt c
//
LOCUS       DASWNJ010000241         8233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_110544, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000241 DASWNJ010000000
VERSION     DASWNJ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 8233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8233
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110544"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="VGB85_04365"
     CDS             complement(<1..1179)
                     /locus_tag="VGB85_04365"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY0133284.1"
                     /translation="MQRASLRRVGRLWAVIAAVGGVSGCGDDLPANTTEGSGSSSTGT
                     DGDTDGPAVTITTPTVTTDEPGTATGPDTSDGSTTVDPPTTGDDSTTTDGPTTTTPGE
                     TTDGINEAPVALVDVYLAKARQVLAPSAAAGVLANDFDPDGDKVEVIASDPLTPGGAS
                     VTMPKDGSFTYFPPADLWGPDSFTYKIHDGKALFASTTVQVKLSPTAIPLWAIAGGKR
                     GFAINGAAPDDYSGRAVQSIGDLNNDGLGDLVVASRNFSGNTGRVYVVFGKTTGGEVS
                     LASLADDEAGFEITGEAAGDLAGSSISGAGDVNGDGIPDILLGAPGAGVKGKSSGTSY
                     VVLGKASSDPVFLEAVALGEGGFSIDGEATQHFSGESVKAAGDVNGDGLADIIVGAYG
                     A"
     gene            1323..2606
                     /locus_tag="VGB85_04370"
     CDS             1323..2606
                     /locus_tag="VGB85_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEY0133285.1"
                     /translation="MTFTSSTRLLVPLTLCLACGDSGGSADSLSATGGASTITSATMT
                     ASGTPTTGDGTMSAGESTAEISGAAGSQTNGDDSTTATGGATSTGETTGEATTESTAG
                     ATTTTTTTSETMTTATDTTGALVPEPCQVEETKINPIPPDVLLVLDKSGSMSMETWDH
                     DANPQTPTVTRWYSLYGVVSSIVTKFDKTVNFGMKLYPFIDAGSYENEGACKVDPGVE
                     VEIAPENAASVLALLPGADIDVLGGTPMETGLKEAFTYLQALPADKQRFALMVADGEI
                     SKTCQGEVFNEALGAVKNAYAKGIATYVVGIDVDPSTSAQLAALAEAGGKPNVNNPDG
                     FYQTSSQIELQAALQQIVDDTLSCVIDVNPEPSEPDLFQVWIGADRIDAVTDCATEDG
                     WVWTKPHIQIELCGAACLELKKQHQVEARYFCTAG"
     gene            complement(2700..3134)
                     /locus_tag="VGB85_04375"
     CDS             complement(2700..3134)
                     /locus_tag="VGB85_04375"
                     /inference="COORDINATES: protein motif:HMM:NF037928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HEY0133286.1"
                     /translation="MEATWFVVADRSQAQLFEVEGTKLKPKLKSLGTIEHPEGRRAEA
                     RRNGASSSSGMRDLLVETNSVQEEQDRKFIRQLIDRLTQAQNQKQFGRLVIAAPADFV
                     GRLRELSARSLSKSVVREIIGDYTNDTIHALQERARQKEWLH"
     gene            3793..6414
                     /gene="gyrA"
                     /locus_tag="VGB85_04380"
     CDS             3793..6414
                     /gene="gyrA"
                     /locus_tag="VGB85_04380"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY0133287.1"
                     /translation="MATGEQKTGINIEDEMRSSFLDYAMSVIISRALPDVRDGLKPVH
                     RRVLYAMDQLRNTYQQPYKKSARVVGDVIGKYHPHGDQSVYDALVRLAQDFAMRYPLI
                     DGQGNFGSIDGDSPAAMRYTEVRMNKLTSELLADLEKDTVDWAPNYDEKELEPTVMPT
                     KVPNLLLNGAVGIAVGMATNIPPHNLRELILACLALIDDPKLQARDLLSYVTGPDFPT
                     GGFIIGHAGIRQAYTTGRGSLTIRAKCEIEEDEKGREAIIVTEIPYQVNKVKLISHIA
                     ELVREKKLEGISDIQDYSDRTGMRIWIQVKRDAAAQVVLNTLYKMSDLQITFGVNMIA
                     IVHGRPLLLSLRDCLGHFIDHRRTVVTRRSRFELKKALERREIVEGLGVAIDAIDRII
                     AIIRASKDPDEARMALCAEPLAGFAGFLERCGRPAPEVEAARGTIYRLNQRQAKAILD
                     MRLQRLTGLERDKIEGEYRELSGIITYLESILADPALLMKVIRDELAAIGTEYGDARR
                     TQVIEDEGEIEAIDLVADEPMIVMLTNNGYIKRLPSGEYRVQGRGGVGIKGGVGRDTT
                     DFVTELFEATAHAHVLLFTNTGRMFRMRVFQLPQGRRDSAGKALVNFLDLKAGERVLG
                     MVPYRDDDVEDGEHYIVMISAQGYIKRTKLDEFSNIRSTGIIAATIEEDDQLIDARLT
                     NGHRHIMLTSARGMAIRFEEKDVRPMGRTARGVRGMDLRSGDRVVGMTVLDADTDAAI
                     LTMCAQGYGKRTPAEDYKVQNRGGKGIITIKVNNRNGPVVGSLAVQTDDQIMLVTNRG
                     KVIRTRVSEISAVGRNTMGVRVIRTVEDERVVAIERLVDQDDASVAEAAPPLERVPED
                     LSETAAEDETEGDDGDA"
     gene            6401..7759
                     /gene="hemL"
                     /locus_tag="VGB85_04385"
     CDS             6401..7759
                     /gene="hemL"
                     /locus_tag="VGB85_04385"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEY0133288.1"
                     /translation="MATRDDSSVGDFVSVKPGDGRAGERSKQLFAAAQEVIPGGVNSP
                     VRAFRSVGGDPPFIRAARGAHLLTEDGVTLIDYVGSWGPAILGHGHPAVVEAVTRAAQ
                     NGLTFGAASAAEVECAELLCRIVPALRGGMVRLVSSGTEATMSALRLARGFTGRTKIV
                     KADGGYHGHADMLLVAAGSGAATLGIPGSGGVPEATVHDTLTVPYNDADAFEQVLLAN
                     PGQVAAIIIEPIAGNMGCVPPQPGYLERLRALCSVHGALLIFDEVMTGFRVALGGAHA
                     RYGIEPDLVCLGKIVGGGMPCGAYGGRKEILQQIAPLGPVYQAGTLSGNPLAVAAGLA
                     TLRILEDSNPYPQLEATTAALVEGIGRHAAKHGVPLTSNRVGAMFTGFFRAGQVTDYT
                     TARGSDLAAFGVFHREMLARGVYLAPSQFEAGFVSTAHDAAVIEHTLAAADEALAAVA
                     NR"
     gene            7814..8212
                     /locus_tag="VGB85_04390"
     CDS             7814..8212
                     /locus_tag="VGB85_04390"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEY0133289.1"
                     /translation="MTEPTKRPQIVERARWFQLTEAASIGIEMAAAIGMGALLGKVME
                     RHVTHWAPWTTFIGLGIGIAAAVFAVIRTARNFTRVIAAEEAAEAEEAARAVAEGRVP
                     PTPAHSHDGGGLGGFEDPGDDRDRPDRGAP"
BASE COUNT         1337 a         3000 c         2735 g         1161 t
ORIGIN      
        1 ggccccgtag gcgccgacga tgatgtcggc gaggccgtcg ccgttgacgt cgccggccgc
       61 cttcacgctc tcgcccgaga agtgctgggt ggcctcgccg tcgatcgaga agccgccctc
      121 gccgagcgcg accgcctcga ggaacaccgg gtcgctgctg gccttgccga gcaccacgta
      181 cgaggtgccc gacgacttgc ccttgacccc cgcgccgggg gccccgagca ggatgtcggg
      241 gatgccgtcg ccgttgacgt cgccggcgcc ggagatcgac gagccggcga ggtcgcccgc
      301 ggcctcgccg gtgatctcga agccggcctc gtcgtccgcg aggctggcga gcgagacctc
      361 gccgccggtg gtcttgccga acaccacgta gacgcggccg gtgttgccgg agaagttgcg
      421 cgacgcgacg acgaggtcgc cgaggccgtc gttgttgagg tcaccgatgg actgcaccgc
      481 gcgaccggag tagtcgtcgg gggcggcgcc attgatcgca aagccgcgct tgcccccggc
      541 gatcgcccac agcgggatcg cggtcgggct gagcttgacc tgcacggtcg tgctggcgaa
      601 cagcgccttg ccgtcgtgga tcttgtacgt gaagctgtcg gggccccaca ggtcggccgg
      661 cgggaaatag gtgaaggagc cgtccttggg catcgtcacc gacgcgccgc ccggcgtcag
      721 cgggtccgag gcgatcacct cgaccttgtc accgtccggg tcgaagtcgt tggcgagcac
      781 gcccgcagcc gccgacgggg ccaacacctg ccgcgccttg gccaaataaa cgtcgaccag
      841 cgcgaccggg gcctcgttga tgccgtcggt cgtctcgccc ggcgtcgtcg tcgtcggccc
      901 gtccgtggtc gtcgagtcgt cgcccgtggt cggcgggtcg accgtcgtcg agccgtcact
      961 ggtatcgggg ccggtcgccg tgcccggctc gtcggtcgtc accgtcggcg tcgtgatggt
     1021 gacggccggg ccgtcggtat cgccatcggt cccggtcgag gagctgccgc tgccctcggt
     1081 ggtgttggcg ggcaggtcgt ctccgcaacc ggagaccccg ccgacggccg cgatgacggc
     1141 ccacagccgg ccgacccggc gaagcgacgc gcgctgcatg gctgtcacac tacccgagga
     1201 gcacggcgcc cgggagcgcg aactcgccct ggaggcccca caagcctggt tggccaacga
     1261 gaactggcgc acagtcgcgc gctgccgcat catcggatcg tttccgcccc ggagatcttg
     1321 ccatgacctt tacttcctcg acccggctcc tcgtccccct gaccctgtgc ctcgcctgtg
     1381 gcgactccgg cggctccgcc gacagcctct cggccaccgg cggcgcgagc acgatcacca
     1441 gcgcgaccat gaccgccagc ggcacgccga ccaccggcga cggcacgatg agcgccggcg
     1501 agtcgacggc cgagatcagc ggggccgccg gctcgcagac gaacggagac gactcgacga
     1561 ccgcgaccgg cggagcgacg agcaccggcg agaccacggg tgaggcgacc accgagtcga
     1621 cggccggcgc gacgaccacc acgacgacca ccagcgagac gatgacgacc gcgaccgaca
     1681 cgaccggcgc gctcgtgccc gagccctgtc aggtcgagga gacgaagatc aacccgatcc
     1741 cgcccgatgt cctgctcgtg ctcgacaagt cgggcagcat gtcgatggag acgtgggacc
     1801 acgacgccaa cccgcagacg ccgaccgtga ctcgctggta cagcctgtac ggggtcgtca
     1861 gctcgatcgt caccaagttc gacaagaccg tgaactttgg catgaagctg taccccttca
     1921 tcgacgccgg ctcctacgag aacgaaggcg cctgcaaggt cgaccccggc gtcgaggtcg
     1981 agatcgcccc ggagaacgct gccagcgtgc tcgcgctgct ccccggcgcc gacatcgacg
     2041 tgctcggcgg caccccgatg gagaccgggc tcaaggaggc cttcacgtac ctgcaagcgc
     2101 tgcccgccga caagcagcgc ttcgccctga tggtcgccga cggcgagatc agcaagacct
     2161 gccagggcga ggtgttcaac gaggcgctcg gcgcggtcaa gaacgcgtac gccaagggca
     2221 tcgcgaccta tgtggtcggc atcgacgtcg acccctccac cagcgcccag ctggccgcgc
     2281 tcgcggaggc cggcggcaag cccaacgtca acaaccccga cggcttctac cagacctcgt
     2341 cccagatcga gctgcaggcc gccctccagc agatcgtcga cgacacgctc agctgcgtga
     2401 tcgacgtcaa ccccgagccg agcgagcccg acctgttcca ggtgtggatc ggcgccgacc
     2461 gcatcgacgc cgtcaccgac tgcgccaccg aggacggctg ggtgtggacc aagccgcaca
     2521 tccagatcga gctgtgcggc gccgcctgcc tcgagctcaa gaagcagcac caggtcgagg
     2581 cccgctactt ctgcaccgcc ggctgagccg ccgctccgga aaatggaagc gcccggcgct
     2641 cgtgagagcg accgggcgcg cagcggctgt cccccgggac agccgcgttc agggggctgt
     2701 cagtgcagcc actccttctg tcgcgcgcgc tcctggaggg cgtggatcgt gtcgttggtg
     2761 tagtcaccga tgatctcccg gaccaccgac ttcgacaggc tgcgggccga cagctcgcgc
     2821 aggcgcccga cgaagtccgc cggggctgcg atcaccagcc gcccgaactg cttctgattc
     2881 tgggcctgcg tcagccggtc gatcagctgg cgaatgaact tgcggtcctg ctcctcctgc
     2941 acgctgttgg tctccaccaa caggtcgcgc atgcccgacg agctgctcgc cccgttgcga
     3001 cgcgcctccg cgcgccgccc ctccgggtgc tcgatcgtcc cgagcgactt gagcttcggc
     3061 ttcagcttgg tcccctcgac ctcgaagagc tgcgcctgac tgcgatccgc gacgacgaac
     3121 caggttgctt ccattcagta cctccatttt tgatgatgat gcaggccggg cggcctatca
     3181 atgcccaatc gcccggtaca cgccgccgac atgatgtggc gagccgtggc gagacgtggc
     3241 gagacgtggg cccacgctgc cgtctgcatg cgtgtggcgg ccgctggccg ggctgcgggc
     3301 tcacccggat gtacttgtcg gacggcacca cgtattcccg gtcgaaccat cgttcggtcg
     3361 ccggccattg ctggcacccc gcgctccgat atgtattaaa aattaccgat tgttcactca
     3421 ttgaaactgc gagagccccg gcgagctccg ggggcagggg cccgcaatgg cgtacttccg
     3481 gggccataaa gcccaattcc gaccggataa atagtcccgc ccgcgacccc ctcgtggagc
     3541 cgatctcggc ctcccgagac gcgccgtccc gcgccggggc cgcgcgtgaa agggggtgga
     3601 aggggggtgg aaaagggggg tggaaaacgg ggtcctcgtg tggctgccca cgccccgaag
     3661 cggcggtggt gcgaagccgg gcgcggacac gtcgattttg ggcccaaacg gccgcggacg
     3721 gcggtttttg ggcaaaatag catggcgggg gcgaccccat tttggtatct tcccggcccc
     3781 ttacgggcac ctatggcgac gggcgagcaa aagaccggga tcaacatcga agacgagatg
     3841 cgtagctcct tcttggacta cgccatgagc gtcatcatca gccgcgctct gccggacgtg
     3901 cgcgacgggc tcaagccggt ccaccgccgc gtcctgtacg cgatggacca gctgcgcaac
     3961 acgtaccagc agccgtacaa gaagtccgcg cgcgtcgtcg gcgacgtcat cggcaagtac
     4021 cacccgcacg gcgaccagtc cgtgtacgac gcgctggtcc ggctcgcgca ggacttcgcg
     4081 atgcgctacc cgctgatcga cggtcagggc aacttcggca gcatcgacgg cgactcgccg
     4141 gcggcgatgc gttacaccga ggtgcgcatg aacaagttga ccagcgagct gctggccgac
     4201 ctcgagaaag acaccgtcga ctgggcgccg aactacgacg agaaggagct cgagccgacg
     4261 gtcatgccga ccaaggtgcc gaacctcctg ctcaacggag cggtcggcat cgcggtcggc
     4321 atggcgacca acatcccgcc gcacaacctc cgcgagctga tcctcgcctg cctggcgctg
     4381 atcgacgacc cgaagctgca agcgcgcgac ctgctgtcct acgtcaccgg cccggacttc
     4441 ccgaccggcg gcttcatcat cggccacgcc ggcatccgcc aggcctacac gaccggccgc
     4501 ggctccctga ccatccgcgc caagtgcgag atcgaagaag acgagaaggg ccgcgaggcg
     4561 atcatcgtca cggagatccc gtaccaggtc aacaaggtca agctgatctc gcacatcgcc
     4621 gagctcgtcc gcgagaagaa gctcgagggc atcagcgaca tccaggacta cagcgaccgc
     4681 accggcatgc ggatctggat ccaggtcaag cgtgacgccg ccgcgcaggt cgtgctcaac
     4741 acgctctata aaatgagcga cctgcagatc accttcggcg tcaacatgat cgccatcgtg
     4801 cacgggcggc cgctgctgct gtcgctgcgc gactgcctcg ggcacttcat cgaccaccgc
     4861 cgcacggtcg tcacccgccg ctcgcgcttc gagctcaaga aggcgctcga gcgccgcgag
     4921 atcgtcgagg gcctcggcgt cgcgatcgac gcgatcgacc gcatcatcgc gatcatccgc
     4981 gcctccaagg accccgacga ggcgcgcatg gccctgtgcg ccgagccgct cgccggcttc
     5041 gccggcttcc tcgagcgctg cggacgccct gcacccgagg tcgaggcggc ccgcggcacg
     5101 atctaccggc tcaaccagcg ccaggccaag gcgatcctcg acatgcgcct gcagcggctg
     5161 accggcctcg agcgcgacaa gatcgagggc gagtaccgcg agctgtcggg catcatcacc
     5221 tacctcgagt cgatcctcgc cgacccggcc ctgctgatga aggtcatccg cgacgagctg
     5281 gctgcgatcg gcaccgagta cggcgacgcg cgccgcaccc aggtgatcga ggacgagggc
     5341 gagatcgaag cgatcgacct cgtcgccgac gagccgatga tcgtcatgct gacgaacaac
     5401 ggctacatca agcgcctgcc gagcggcgag taccgcgtgc agggccgcgg cggcgtcggc
     5461 atcaagggcg gcgtcggccg cgacaccacc gacttcgtca ccgagctgtt cgaggcgacc
     5521 gcccacgccc acgtcctgct gttcaccaac accggccgca tgttccgcat gcgcgtgttc
     5581 cagctgccgc agggccgccg ggactcggcc ggcaaggcgc tcgtcaactt cctcgacctc
     5641 aaggccggcg agcgcgtgct cggcatggtc ccgtacaggg acgacgacgt cgaggacggc
     5701 gagcactaca tcgtgatgat ctccgcgcag gggtacatca agcggaccaa gctcgacgag
     5761 ttctccaaca tccgctcgac cggcatcatc gccgcgacga tcgaggagga cgaccagctg
     5821 atcgacgcgc gcctgaccaa cggccaccgt cacatcatgc tgacctccgc gcgcggcatg
     5881 gcgatccgct tcgaggagaa ggatgtccgc ccgatgggac gcaccgccag aggcgtgcgc
     5941 ggcatggacc tccgaagcgg cgaccgcgtc gtcggcatga ccgtgctcga cgccgacacc
     6001 gacgcggcga tcctgacgat gtgcgcccag ggctacggca agcgcacccc cgccgaggac
     6061 tacaaggtcc agaaccgcgg cggcaagggc atcatcacga tcaaggtcaa caaccgcaac
     6121 ggcccggtcg tcggctcgct ggcggtccag accgacgacc agatcatgct cgtcaccaac
     6181 cgcggcaagg tcatccgcac ccgcgtgtcg gagatctccg cggtcggccg caacaccatg
     6241 ggcgtccggg tgatccgcac cgtcgaggac gagcgcgtcg tggcgatcga gcgcctcgtc
     6301 gaccaggacg acgccagcgt cgccgaggcc gctccgccgc tcgagcgcgt gcccgaggac
     6361 ctctccgaga ccgccgccga ggacgagacg gagggcgacg atggcgacgc gtgatgactc
     6421 atccgtcggc gacttcgttt cggtaaaacc cggagacggc cgcgccggcg agcgcagcaa
     6481 gcaattgttc gcggcggccc aggaggtgat cccgggcggc gtgaacagcc ccgtgcgggc
     6541 gtttcgctcg gtcggcggcg acccgccgtt catccgcgcg gcccgtggcg cccacctgct
     6601 gaccgaggac ggcgtcacgc tgatcgacta cgtcggatcg tggggccccg cgatccttgg
     6661 acacggccac ccggccgtcg tcgaggcggt cacccgcgcg gcccagaatg gcctcacctt
     6721 cggcgccgcg agcgcggccg aggtcgagtg cgccgagctg ctgtgccgca tcgtgccggc
     6781 cctgcgcggc ggcatggtcc gcctggtgtc ctcgggcacc gaggcgacca tgtcggccct
     6841 gcgcctggct cgcggcttca ccggccgcac caagatcgtc aaggccgacg gcggctatca
     6901 cggtcacgcc gacatgttgc tggtcgccgc cggctccggc gcggccactc tcggcatccc
     6961 cggctccggc ggcgtgccgg aggcgaccgt gcacgacacg ctgacggtcc cctacaacga
     7021 cgccgacgcc ttcgagcagg tgctgctcgc caaccccggc caggtcgcgg cgatcatcat
     7081 cgagccgatc gccggcaaca tgggctgcgt gccgccgcag ccgggctacc tcgagcgcct
     7141 gcgcgccctg tgcagcgtgc acggggccct gctgatcttt gacgaggtga tgaccggctt
     7201 tcgcgtcgcg ctcggcggcg ctcacgcgcg ctacggcatc gagccggacc tcgtctgcct
     7261 cggcaagatc gtcggcggcg gcatgccctg cggcgcctac ggcggccgca aggagatcct
     7321 ccagcagatc gcgccgctcg ggccggtcta ccaggccggc accctcagcg ggaacccgct
     7381 ggccgtcgcc gccggcctcg ccaccctgcg catcctcgag gacagcaacc cctacccgca
     7441 gctcgaggcc accaccgccg cgctggtcga gggcatcggc cgccacgcgg cgaagcacgg
     7501 cgttccgctg accagcaacc gcgtcggcgc catgttcacc ggcttcttcc gggccggtca
     7561 ggtcaccgac tacacgaccg cccgcggcag cgacctcgcg gccttcggcg tgttccaccg
     7621 cgagatgctg gcccgcggcg tgtacctggc cccctcgcag ttcgaggccg gcttcgtctc
     7681 gaccgcccac gacgcggccg tcatcgaaca cacgctcgcc gccgccgacg aggccctcgc
     7741 cgcggtcgcc aaccggtagc cacttcatcg gccgctggcc ccgggcacgc gctcggggta
     7801 catcaggttc gccatgaccg agcccaccaa gcgtccgcag atcgtcgagc gggcgcgctg
     7861 gttccagctg accgaggccg cttcgatcgg catcgagatg gccgccgcga tcggcatggg
     7921 cgcgctcctg ggcaaggtga tggagcgcca cgtcacccac tgggccccgt ggacgacctt
     7981 tatcgggctc ggcatcggca tcgccgcggc cgtgttcgcg gtcatccgca ccgctcgtaa
     8041 cttcaccagg gtcatcgccg ccgaggaggc cgccgaggcc gaggaggccg cccgcgccgt
     8101 cgccgagggc cgcgtgccgc cgactccggc gcattctcac gatggcggcg ggctcggcgg
     8161 cttcgaggac ccgggcgacg accgtgaccg gcccgaccgc ggtgctccat gagcggcccc
     8221 gggatcgcca cgc
//
LOCUS       DASWNJ010000242         2076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_4893, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000242 DASWNJ010000000
VERSION     DASWNJ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4893"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VGB85_04395"
     CDS             <1..488
                     /locus_tag="VGB85_04395"
                     /inference="COORDINATES: protein motif:HMM:NF021456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HEY0133290.1"
                     /translation="YIEESRARAKPLPNLEDPRAPIQRWDDRPEPVGTGACAQNFGPR
                     VRHGVEVDEATNTIRRITPQFFNDAFPGMIAPAVAQGDRVVVRGVREDGPLSFVVPPT
                     SLRVDVRVGDDGGVRTPAIDQIGIEADAGLVFITYRYPFRYVLTPHQRRHCRLSLAPA
                     S"
     gene            500..1546
                     /locus_tag="VGB85_04400"
     CDS             500..1546
                     /locus_tag="VGB85_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133291.1"
                     /translation="MFAVMKHWDIMIGLDAHTCWPPGSPYPIGPKPYIVCMLMTGTML
                     TAQMAFTAVTFNGWTMKRGTDIGPLIPHIGQPSLLTPLDMLFSSSASYFGPSSHTVEG
                     APVAAALFMFANPNLNCHFFGPAPTGIVLCFTTHYVGMSIFDILFGVISMAVAIVIDV
                     ATGAVAGAASKAAQKTIIKPITRRVSAKLASRKAAKAATPSTGALTPGASTTPSTGAL
                     TPGTSAPAKAPPGTWQSAKPSQPAGSQGPSAPAKAPPGTWQPAKPSQPAGSQGTGNPP
                     GPQFGGHRPPVKPPKKRKTRKQFALDLGLEAFGELALGLPFSVVNELTFELLSRTGVE
                     EFGKREDEKSSEET"
     gene            1548..>2076
                     /locus_tag="VGB85_04405"
     CDS             1548..>2076
                     /locus_tag="VGB85_04405"
                     /inference="COORDINATES: protein motif:HMM:NF021456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HEY0133292.1"
                     /translation="MPPDAAVTATQLSCTLPDGSPALVVLARRTYAIDDAGRCTLAHE
                     QPGLLTEPVWDPVVPDLLLADHDLFFHKLRTDVIVQGHVHGDGRATRLTAGVSVGRSH
                     KRMMVLGERRCTLGRTGAVMISEPAAIDRVPLRYTHAYGGRDAVHEAVHGHPLRGDPE
                     FAAMTESELDAASPYL"
BASE COUNT          324 a          786 c          652 g          314 t
ORIGIN      
        1 tctacatcga ggagtcccgc gcgcgcgcga agcccttgcc gaacctcgag gatccccgcg
       61 cgccgatcca gcgctgggac gaccgcccgg agccggtcgg caccggggcg tgcgcgcaga
      121 acttcgggcc gcgggtgcgt cacggggtcg aggtcgacga ggccacgaac acgatccgtc
      181 gcatcacccc gcagttcttc aacgacgcgt tcccggggat gatcgccccg gccgtcgccc
      241 agggcgatcg cgtggtcgtc cgcggcgtac gcgaggacgg cccgctgagc ttcgtggtcc
      301 cgccgacgtc cctgcgcgtc gatgtccgcg tcggcgacga cggcggcgtg cgcacgccgg
      361 cgatcgatca gatcgggatc gaggccgacg ccggcctcgt cttcatcacc taccgatatc
      421 cctttcgcta cgtgctgaca ccgcatcagc gtcgccactg ccggctgtcc ctcgccccgg
      481 cgagctgagg aggatcgtca tgttcgccgt catgaagcac tgggacatca tgatcgggct
      541 cgacgctcac acgtgctggc ctcccggttc accgtacccg atcggcccga agccctacat
      601 cgtgtgcatg ctgatgaccg gcacgatgct caccgcccag atggcgttca cggccgtgac
      661 cttcaacgga tggacgatga agcgcggcac cgacatcggc ccgctgatcc cccacatcgg
      721 ccagccgagc ctgttgacgc cgctcgacat gctctttagc agctccgcct cgtacttcgg
      781 accgtcctcg cacaccgtcg agggggcgcc ggtcgccgcg gcgctgttca tgttcgccaa
      841 cccgaacctc aattgtcact tcttcggccc ggcgccgacc ggcatcgtat tgtgcttcac
      901 gacgcactac gtcgggatgt cgatcttcga catcctgttc ggcgtcatct cgatggccgt
      961 cgccatcgtc atcgatgtcg cgaccggggc ggtggcgggc gccgccagca aggccgcgca
     1021 aaagacgatc atcaagccga tcaccaggcg cgtctcggcc aagctcgcct cgcgaaaagc
     1081 ggccaaggcc gccaccccct ccaccggcgc gctcacacct ggcgcctcca cgacgccgag
     1141 caccggcgca ctcacgcccg gaacctccgc ccccgcgaag gctccccccg ggacctggca
     1201 atctgccaaa ccctcgcagc cggccggctc gcaaggcccc tccgcccccg cgaaggctcc
     1261 ccccggaacc tggcaacctg ccaaaccctc gcagccggcc ggctcgcaag gcactggaaa
     1321 tccgccgggc ccgcagttcg gaggacaccg gccgcccgtc aaaccaccga agaagcggaa
     1381 gaccaggaag cagttcgcgc tcgacctggg cctcgaggcc ttcggcgagc tggcgctcgg
     1441 cctcccgttc tcggtggtga acgagctgac cttcgagctg ctctccagga ccggggtcga
     1501 ggaattcggc aagcgcgagg acgagaagtc gtccgaggag acctgacatg ccgcccgacg
     1561 cagccgtcac cgcaacgcag ctctcgtgca ccctccccga cggctcgccg gcgctcgtgg
     1621 tgctcgcccg ccggacctac gcgatcgacg acgccgggcg ttgcacactg gcgcacgagc
     1681 agcctggcct gctcaccgag ccggtgtggg accccgtggt gcccgacctg ctgctcgccg
     1741 atcacgacct gttctttcac aagttgcgca ccgacgtgat cgtgcaaggt catgtccatg
     1801 gtgacggtcg ggcgacgcgg ctcaccgccg gcgtcagcgt cggccgctcg cacaagcgca
     1861 tgatggtgct cggcgagcgc cgctgcaccc tcggccgcac cggcgcggtg atgatctccg
     1921 agcccgcggc gatcgatcgc gtgccgctgc gttacaccca cgcgtacggc gggcgtgatg
     1981 cggtccacga ggcggtccac ggccacccct tgcgcggtga tccggagttc gcggcgatga
     2041 ccgagtcgga gctcgacgcc gcatcgccct atctgt
//
LOCUS       DASWNJ010000243         1505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_320411, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000243 DASWNJ010000000
VERSION     DASWNJ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320411"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            839..1402
                     /locus_tag="VGB85_04410"
     CDS             839..1402
                     /locus_tag="VGB85_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133293.1"
                     /translation="MRAILYTLVGWILLASVAGLGASFGLTVMLPATSAVLVTHLAFS
                     RDLSLPTGLAVAIALGYLEDLHQGTPVGALSLAHGLAFLLTVWAGTRIAVEGAIVRAL
                     AAGVVAAVIDLLTFAEFALLADRLGIEVRTLVRALPTLRWHALATVLAAPAVGLVADL
                     VVGTWDRLMGRSNPRPRPLAASSWYRS"
BASE COUNT          174 a          540 c          545 g          246 t
ORIGIN      
        1 acgcgggtcc tcccagggga catgctcgcc gccccatctg tcccgaaaga cagggtcgtt
       61 tcaccgaccg ttcatcgcgt cgacggaggt tgacggggcg tctgggcgtg acccctgggt
      121 cctcccgctg cttcccccgc cagtcggccc ctgccccccg ggcgggcatc gtcgcggccc
      181 cgcttggcgt caggacgatg ccctcgcggc gccggacatg cttcgtcggg cgcgtccggg
      241 gcatgcagtc gcttccactg agcgccacgt gttcgtcggt gcgggaccgc ggggcggaca
      301 tggtcgtggc caagcttgtc gtgatgacga tgcagtcgag gggccggaca tgcttcatcg
      361 ggtgtcgcga ccggggcaca gcgatccttc ggcggggttg tccgtggtcg tcgctgcgtg
      421 cctgcggggc ggacatcgtt gggcgcgacg aagccacgcg gctgcgctcg ttgtctgcag
      481 gtggggcgtt gcgagtcgct gctcgacgtg tggtgagggc ggccggtcgc gcgacgatgc
      541 aggccgttgc gacaagcggg gcgcatacgg tgcctgtccg gggggcaggg gccggctggc
      601 gggggaagca gcgggaggac ccagggctcg cgcgcagccg ccccaccgcc agtcgagagc
      661 ggcgagacag cgacggtgaa agagacatgt ccttccggac aggtggggcg gcgagcacgt
      721 gctctgggag gacccgcgtc gcagggaccc gcagcccgtc ggcccccccc cggcccacac
      781 agcctcgccc cgcgcccctc gatctcgcga tcaggcatcc cggacccgcc acgagaccat
      841 gagggccatt ctctacacat tggtcgggtg gatcctgctg gccagcgtgg ccggtctcgg
      901 cgcgagcttc ggtctcacgg tgatgctgcc cgcgacatcc gcggtgctgg tgacgcacct
      961 ggccttctca cgcgacctct cgctgccgac cggcctcgcc gtggcgatcg ctctcggcta
     1021 cctcgaggac ctgcatcagg gcacccctgt cggcgcgctg tcgctggccc acggtctcgc
     1081 cttcctgctg accgtgtggg ccggcacgcg gatcgcggtc gagggcgcga tcgtgcgcgc
     1141 gctcgcggcc ggcgtcgtgg ccgccgtgat cgacctgctg accttcgccg agttcgcgtt
     1201 gctcgccgac cgtctcggca tcgaggtccg cacgctcgtg cgggccctgc cgaccctgcg
     1261 ctggcacgcg ctggcgacgg tgctcgccgc gccggcggtc ggcctcgtcg ccgacctcgt
     1321 ggtcggcacc tgggaccgcc tgatgggtcg gagcaacccg cgcccgcggc ccctcgccgc
     1381 ctcttcatgg taccgatcgt gacgactacg atatgcaagt gaccgacatt cgccagcccg
     1441 aggacctgcg ccagctgcgg ccgcggctgc aggcgatggt gctgctcgtc gtgctcgcct
     1501 tcgtc
//
LOCUS       DASWNJ010000244         2884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_605233, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000244 DASWNJ010000000
VERSION     DASWNJ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605233"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VGB85_04415"
     CDS             <1..333
                     /locus_tag="VGB85_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HEY0133294.1"
                     /translation="AIVTYTVSGGIARLVSDYRPRVPIIALTPSSATYQTLAMYWGVT
                     PVLFTLSSAEGRSIFQDVDRLLLGQGLMARGDRMVLTLGYPLKDHKSVNLLKIHSVGE
                     SLPLPPNV"
     gene            384..465
                     /locus_tag="VGB85_04420"
     tRNA            384..465
                     /locus_tag="VGB85_04420"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:418..420,aa:Leu,seq:aag)
     gene            575..1450
                     /locus_tag="VGB85_04425"
     CDS             575..1450
                     /locus_tag="VGB85_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0133295.1"
                     /translation="MTDPSQSPAVAVVAVIGAGTMGHGIAHVCAMAGYQTRLFDVRQP
                     LVDAGLAKISANLDAGVSKGKVTAEQKAAALANLRGESQLQPAVFGADLVIEAVPEKL
                     ELKRSLFQEVAAITGTSAILASNTSSLSLADIAAAVPGPERVIGTHFFNPVHVMKLLE
                     IVVAPSTSPATLAAIQAFGAKIGKDCIVVKDSPGFATSRLGLVIGLEAIRMVEEGVAS
                     AEDIDKAMCLGYGFPMGPLRLTDLVGLDVRLSIADYLSEKLTVGAHFKPPALLREMVA
                     QGKLGKKSGEGFYKW"
     gene            complement(1438..>2884)
                     /locus_tag="VGB85_04430"
     CDS             complement(1438..>2884)
                     /locus_tag="VGB85_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0133296.1"
                     /translation="FPCLAAPDDTPLLTVDGVTLTRRALALACARHVAWLRAAGVQQG
                     DHVGVWTQPDLATPVALAANALFGAVSVPINPRLGTREAAHIAADARPRLCLAADPSQ
                     IKDSPLGPAASLAHLSDPTCAISPVPERRADATPLLILYTSGTTGLPKGAVLGAAGVA
                     ANLDALARVWRWGPEHAVVHALPLFHVHGLVLGWFGSLRVGGRMVWLPRFDPTALAAA
                     LTADCMLFAVPTMYHRLAEVAEADPAVASGLAAARLLVSGSAALGLREQRRITGLCGH
                     GVHERYGLTETLINCAVPADGPPQPGRVGPPLPGVELRLVDDERRPLATSDDTTIGEI
                     AVRGPQLFLGYLNHPVATDPDGWFHTGDLATRDAEGSLRIVGRRATDLIKSGGFKIGA
                     GEIEACLAEHPEISEVAVIGVDDPDLGERIVAFVVPRDLAAPPPAEALIEFVAAALAP
                     HKRPRRVVFLEALPRNAMGKVLKPALRTHHL"
BASE COUNT          419 a          964 c         1099 g          402 t
ORIGIN      
        1 gcgatcgtga cgtacacggt ctccggcggg atcgcccgcc tcgtcagcga ctaccgtccg
       61 cgcgtgccga tcatcgccct gacgccgagc tcggcgacct accagaccct ggcgatgtac
      121 tggggcgtca cgccggtgct gttcacgctg tcgagcgccg aggggcgcag catcttccag
      181 gacgtcgacc gcctgctgct cggccagggc ctgatggccc gcggcgaccg catggtcctg
      241 accctgggct accccctcaa ggatcacaag tccgtgaact tgctcaagat ccactccgtc
      301 ggagagagct tgcctctgcc cccgaacgtg tgatacccac tccatccact cggcgcccac
      361 ccaaaggggt accgagaaac cgcgcgagga tggcggaatt ggtagacgca ctagactaag
      421 gatctagcgg ggcgacctgt gggggttcga gtccccctcc tcgcacaagc ccagagacgc
      481 cggtaccgag aggtcccggc gtttcttttt tggttcgcgc ctgttcatgt ttttctctcg
      541 gacaatgcac cgccagggct aagctcggcc gcgcatgacc gatccgtcgc agtctcccgc
      601 ggtcgccgtg gtcgccgtca tcggcgccgg gaccatgggc catggcatcg cccacgtgtg
      661 cgccatggcc ggctaccaga cccgcctgtt cgacgttcgc cagcccctgg tggacgccgg
      721 gctggcgaag atctccgcga acctcgacgc gggggtcagc aagggcaagg tgaccgccga
      781 gcagaaggcc gcggcgctcg ccaacctgcg cggcgagtcg cagctgcagc cggcggtgtt
      841 cggcgcggac ctggtgatcg aggcggtccc ggagaagctc gagctcaagc gctcgctgtt
      901 ccaggaggtc gcggcgatca cggggacgtc ggcgatcctc gccagcaaca ccagctcgct
      961 gtcgctggcc gacatcgccg cggcggtgcc gggacccgag cgcgtgatcg gcacgcactt
     1021 ttttaatccc gtgcacgtga tgaagctgct cgagatcgtg gtcgcgccgt cgacctcgcc
     1081 ggcgacgctg gcggcgatcc aggcgttcgg cgccaagatc ggcaaggact gcatcgtcgt
     1141 caaggactcg cccggcttcg cgacgtcgcg gctcgggctg gtgatcggcc tcgaggcgat
     1201 ccgcatggtc gaggaggggg tcgcgtccgc ggaggacatc gacaaggcga tgtgcctcgg
     1261 ctacggcttc ccgatgggcc cgctgcgcct gaccgacctc gtcggcctcg acgtgcgcct
     1321 gagcatcgcc gactacctga gcgagaagct caccgtcggc gcccacttca agccgcccgc
     1381 gctgctgcgc gagatggtcg cgcagggcaa gctcggcaag aagtcgggcg agggctttta
     1441 taagtggtga gtccgtagcg cgggcttgag gaccttgccc atcgcgttgc gcggcagggc
     1501 ctcgaggaag acgacccggc gcggccgctt gtgcggggcc agggccgcgg cgacgaactc
     1561 gatcagggcc tcggcggggg gcggcgcggc gaggtcgcgg gggacgacga aggcgacgat
     1621 gcgctcgccg aggtcggggt cgtcgacgcc gatcacggcg acctcggaga tctccgggtg
     1681 ctcggcgagg caggcttcga tctccccggc gccgatcttg aagccgccgc tcttgatcag
     1741 gtcggtcgcg cgccggccga cgatccggag agatccctcg gcgtcgcgcg tggcgaggtc
     1801 accggtgtgg aaccagccgt cggggtcggt ggcgacgggg tggttgaggt aaccgaggaa
     1861 cagctgcggg ccgcgcacgg cgatctcgcc gatcgtggtg tcgtcgctgg tggcgagcgg
     1921 gcggcgctcg tcgtcgacga ggcgcagctc gacgccaggc aacggcgggc cgacgcggcc
     1981 aggctgcggg gggccgtcgg ccgggacggc gcaattgatg agagtttcgg tcaggccgta
     2041 gcgctcgtgg acgccgtggc cgcatagccc ggtgatccgc cgttgctcgc gcaggccgag
     2101 cgcggcggag cccgagacga gcaggcgcgc cgcggcgagg ccggacgcga ccgcggggtc
     2161 ggcctcggcg acctcggcga gccggtggta catcgtcggc accgcgaaca acatgcagtc
     2221 cgcggtcagg gcggccgcga gggcggtcgg gtcgaagcgc ggcagccaga ccatgcgccc
     2281 gccgacgcgc aggctgccga accaccccag cacgaggccg tgcacgtgga acagcggcag
     2341 cgcgtgcacg acggcgtgct ccgggcccca gcgccagacc cgggccagcg cgtcgaggtt
     2401 ggcggccacg cccgcggcgc cgagcacggc ccccttgggt aggccggtgg tgcccgaggt
     2461 gtagaggatc agcaggggcg tggcgtcggc cctgcgctcc gggacaggtg agatcgcaca
     2521 ggtcggatcg gacaggtggg cgagcgaggc ggcggggccg agcgggctgt ccttgatctg
     2581 cgaggggtcg gcggcgaggc acagccgcgg gcgcgcgtcg gcggcgatgt gggcggcctc
     2641 ccgggtaccg aggcggggat tgatcgggac gctgaccgcc ccgaacagcg cgttggcggc
     2701 cagcgcgacc ggggtcgcga ggtcgggctg cgtccagacg ccgacgtgat caccctgctg
     2761 cacgcccgcg gcgcgcagcc acgcgacgtg gcgcgcgcag gcgagggcga gggccctgcg
     2821 ggtcagcgtg acgccgtcga cggtgagcag cggggtgtcg tcgggcgcgg cgaggcaggg
     2881 gaac
//
LOCUS       DASWNJ010000245         2544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_675247, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000245 DASWNJ010000000
VERSION     DASWNJ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675247"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VGB85_04435"
     CDS             complement(<1..731)
                     /locus_tag="VGB85_04435"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEY0133297.1"
                     /translation="MPLSVVVACLAFAGLTTPQPAPQPILGGEVVAACGWPTVVSVGG
                     FCSGTLIHPQVIAYAAHCGDEVPWIRFTDEIESSERREVIPEKCGVHPVGSFGFGTDY
                     AYCRLAEPITDIAITPPLMGCETEELLKIGQKVTLVGFGQSDDPDMNYGIKRFVDTQI
                     NGFSWDEVFVGDAEKGACYGDSGGPAMVQLADGSWRSFAVTSWGKSGCGAGNYFSLLH
                     RGVDWIETETGLDVTPCHDSLGNWDP"
     gene            complement(958..1320)
                     /locus_tag="VGB85_04440"
     CDS             complement(958..1320)
                     /locus_tag="VGB85_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133298.1"
                     /translation="MRDTKLIHVFGFFAAVLCASVPGCGDDNDDGGSTNASVTAGDGN
                     AAEIQSTCDTYCNKSQTCDDGETLEQCVADCKAQLGDCMADEQDQVMQELRDCAQESC
                     DDFGSCTFGTGLQCTFGI"
     gene            complement(1507..2499)
                     /locus_tag="VGB85_04445"
     CDS             complement(1507..2499)
                     /locus_tag="VGB85_04445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY0133299.1"
                     /translation="MHDIVAAACFRLIQVLLSPLGAVGYVIFVARLIAYSRESGASAT
                     VLASLYTRYMQHVLGSRRDEPAARLMAVMPNVPALGLLLSTAPTRVAHAVTGHVPRIY
                     RYPYPGEPPIVHQSAARTTFSDDVVARHLVHADQLVILGAGFDTRAYRLPAGASVRCF
                     EVDAPPTQAYKREMLAKAGVDPGPVRFVAADFATEDWFAKLVAAGFDPDRPGVFIWES
                     VTMYLPREAVVDALRKISRTAPGTVVAFDYMSAELLASRSLFLRYARAVMAASGEAWR
                     FGIDNTPPARARVAELLTACGLVLAEQRNFGPETARTRAPAGFAVAVVPPAGYR"
BASE COUNT          415 a          879 c          876 g          374 t
ORIGIN      
        1 gggtcccagt tgccgaggct gtcgtggcac ggcgtcacgt cgaggcccgt ctcggtctcg
       61 atccagtcga ccccgcggtg cagcagcgag aagtagttgc ccgcgccgca gcccgacttg
      121 ccccacgacg tgaccgcgaa gctgcgccag ctgccgtcgg cgagctggac catcgccggc
      181 ccgccggagt cgccgtagca ggcgcccttc tcggcgtcgc cgacgaacac ctcatcccac
      241 gagaagccat tgatctgggt gtcgacgaag cgcttgatcc cgtaattcat gtcggggtcg
      301 tcgctctgac cgaagccgac cagcgtcacc ttttgcccga tcttgagcag ctcctcggtc
      361 tcgcagccca tcagcggcgg cgtgatcgcg atgtcggtga tcggctcggc gaggcggcag
      421 tacgcgtagt cggtgccgaa gccgaacgag ccgaccggat gcaccccgca cttctccggg
      481 atcacctcgc gccgctcgct ggactcgatc tcgtcggtga agcggatcca cggcacctcg
      541 tcgccgcagt gagccgcgta cgcgatcacc tgcgggtgga tcagcgtgcc cgagcagaaa
      601 ccgccgacgc tgaccaccgt cggccagccg cacgcggcga cgacctcacc accgaggatc
      661 ggctgcggcg caggttgggg ggtggtcaat ccggcgaacg caaggcaagc aacaacgaca
      721 gagagcggca tggcggcgag tatccgtccg caaacgcggc tcgtctagcc ccgtgcgggc
      781 ccgtgctgtc ccgtgccgat cggacctcgc tctgacattt tgacaccgct gtgacagccg
      841 atctcggcca gctgaccgga aacaatgtca accggccgcg tctcctcggt tccggaatga
      901 ccgcgagtcc ccgggacccg cggtcttcga agtggcgcgg agcctcgcag cgggcgctca
      961 gatgccgaac gtgcactgca ggccggtgcc gaaggtgcag ctgccgaagt cgtcgcagct
     1021 ctcctgggcg cagtcgcgga gctcctgcat cacctggtcc tgctcgtcgg ccatgcagtc
     1081 gccgagctgc gccttgcagt cggcgacgca ctgctcgagc gtctcgccgt cgtcgcaggt
     1141 ctgcgacttg ttgcagtagg tgtcgcaggt cgactggatc tcggccgcgt tgccgtcgcc
     1201 ggccgtgacc gaggcattgg tggagccgcc gtcatcgttg tcgtcgccac atccggggac
     1261 gctcgcgcac agcacggcag cgaagaaacc gaacacgtga atcaactttg tgtctcgcat
     1321 gccagcggct atgccctgtg atcggcagca cgcaagcggt gtgcgctgcg ttcgttcgag
     1381 gcaccgccaa tgatggcctg gaacgcgtgt caggggcggt tatccgccgt ctacgcgatc
     1441 gtcgccgcgg agagcgcggc accaccactc ctcgtattgc tggcacagat ccctcacgga
     1501 cggcgatcag cggtagcccg ccggcggcac gacggcgacg gcgaagcccg cgggcgcgcg
     1561 tgtgcgggcc gtctcgggcc cgaagttgcg ctgttcggcg agcacgaggc cgcaggcggt
     1621 caggagctcg gcgacgcggg cacgggccgg cggcgtgttg tcgataccga agcgccacgc
     1681 ctcaccgctg gcggccatca cggctcgcgc gtagcgcagg aacagcgagc gcgacgcgag
     1741 cagctcggcg gacatgtaat cgaaggcgac gacggtgccg ggtgccgtcc gcgagatctt
     1801 gcgcagcgcg tcgacgaccg cctcgcgcgg gagatacatc gtcaccgact cccagatgaa
     1861 cacacccggg cggtcgggat cgaagcccgc ggcgacgagc ttcgcgaacc agtcctcggt
     1921 cgcgaagtcg gccgcgacga agcggaccgg gcccgggtcg acgccggcct tcgccagcat
     1981 ctcgcgcttg tatgcctggg tgggcggcgc gtcgacctcg aagcaacgaa cgctcgcgcc
     2041 ggccggcagg cggtacgcgc gggtgtcgaa gcccgcgccg aggatcacga gctggtcggc
     2101 gtgcacgagg tgccgcgcga ccacgtcatc agaaaaggtc gtgcgcgccg ccgactgatg
     2161 cacgatcggc ggctcgcccg ggtaaggata acggtagatc cgcggcacat ggccggtcac
     2221 cgcgtgggcc acccgcgtcg gcgcggtcga cagcagcagg ccgagcgcgg gcacgttcgg
     2281 catcaccgcc atcagccgcg ccgcaggctc gtcgcgccgt gagccgagca cgtgctgcat
     2341 atagcgggtg tagagcgacg cgagcacggt cgccgacgcg ccggactcgc ggctgtacgc
     2401 gatcagccgg gccacgaaga tcacgtaacc gaccgcgccg agcggcgaca gcaggacctg
     2461 gatgaggcga aaacaggccg cggcgacgat gtcgtgcatg atgccccgcg agtctactcc
     2521 cgtctcccct cctcgcatat gcta
//
LOCUS       DASWNJ010000246        10793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_145795, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000246 DASWNJ010000000
VERSION     DASWNJ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 10793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10793
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145795"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VGB85_04450"
     CDS             complement(<1..377)
                     /locus_tag="VGB85_04450"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0133300.1"
                     /translation="MTTRLSCVLLSLAVAPACGSRRIAGYLEEFESWSETASETGESD
                     TTQGPGSSGAPGSSESGGSTAPHDPDSTSTSTSTTDETTSGSSGEPTAQCGNGMLEVF
                     GPVAEECDDGNLDPEDGCSDSCAA"
     gene            complement(381..1247)
                     /locus_tag="VGB85_04455"
     CDS             complement(381..1247)
                     /locus_tag="VGB85_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133301.1"
                     /translation="MFTSHRVALLLTLAPASGVLSTEAAEYNAKAMHFYKAGELALAV
                     DEFYAAYQAMPDPRRDLAGREQVLGSLRGTLLDLYDRTGEAAVLCRLQGILDEHLRQL
                     TAAFPDAPNRLEIHGARERHAEVTRLLAALAPDVCAPPPPPAPLLPVTPPPAPPPTPA
                     VAPPSDVTDATPRHMQIAGGVMVPLGLVAFAVVGGVASRHGRSTAEADAIHDELATRP
                     RTVDDRTRLRELLAATHRDEGIIIGSAVAGAALLTVGTVLLVRGTLQRRRIHLGLDVR
                     REQVAFTLSGEF"
     gene            complement(1247..2716)
                     /locus_tag="VGB85_04460"
     CDS             complement(1247..2716)
                     /locus_tag="VGB85_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0133302.1"
                     /translation="MSRSGAQRSPIELVTVGDFDPAADSAIEYDFIGRKIAGRYVVRA
                     QIGGGGMADVFQATDEVLGVDVAIKLLKPRLASDDLRARMVQEARAAAQVRHANLVRV
                     FGSGSLDATAFIVMELLAGPNLEQYLRERPDQRLAWDEALALLLPAMEALHAIHERGY
                     IHRDIKPGNILVTREAGCPPSAVVIDLGLVKPDRALRTADSPPTTEAGRMLCTPGYTS
                     PEQALGKPVDRRSDVYSMAITLYRVLAGRLPFHEARGKPIAAVLAGHIHHEPTRLTVA
                     AGTTFIPPAVTAVVESALQKDPALRPQTMRAFAEALRAAASVATAVPAGRVVRVRWAL
                     LACGLCIAATYLLTSRAQPPACSQTAANVTPTAHHDDPFQPPVPVPLASLALPDVAST
                     DHAATRPSHASDRVAAARRVMSRHEDAVQRCFHRYGDRAPRLPVQVSVDRRGHISATV
                     IDAETTPLGDCVSAALASATLPAGTPTSFVHTYRRRGAP"
     gene            3188..4066
                     /locus_tag="VGB85_04465"
     CDS             3188..4066
                     /locus_tag="VGB85_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133303.1"
                     /translation="MTLSPGGDTLVAGSVAGRAVFGVEGALRVVEVGANCSAELGPLL
                     AIAAKGLRMAGDGSLLAAHERFALVDFGHQRDGMRFYGLPAVTLLGQHQVDLISEWIR
                     EMDVDDAGTRWSRMSHDSADPLIDAGEVAGPWEPPYLGFGVQSPRLAPDGLHYVVGRS
                     RYSFETFARHTTIYGLDKKLVALEGAPVGFLDNEHILFNRYDEESDDFSGAVIIDLAG
                     DTVNTTELPELTELFRLGTGEILGRRTQDERWAVFEPSTGAVLWTAGEGAQVALVGTN
                     QLIGLVEGRVELVRWR"
     gene            complement(4207..6564)
                     /locus_tag="VGB85_04470"
     CDS             complement(4207..6564)
                     /locus_tag="VGB85_04470"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0133304.1"
                     /translation="MSYNRRFAPLTSIALLTLQLACPGCADDTAGTTETASESSSGTT
                     GTGPDGSTGTPTTASEPTTPTSTQGEGSMTQASDPTASTTDATTSTTDATSTTTTATS
                     TTTTDGTTSSTSTTDGTTTTGTTDASDTSGTSGTSGDFEAMCGDGIFDPHFEECDDGE
                     NNGPGNFCLDHCKLNVCGDADVGPGEQCDDGPNNGPGEACLANCQGNVCGDGDIGPGE
                     ECDNGANNDDAAACKSDCTQAFCGDGIVGPMEGCDDGNDVDNDDCSNACVAAACGDAV
                     VNAQEACDDGNDVDTDLCTSACTNATCGDGFVQPPVGEQCDAGPDNSDAGDCTLACVP
                     GVCGDGLVHDQGSGEEECDNGLDNGPGQGCLASCKFNVCGDGDLGPGEGCDDGNNVGA
                     DGCSPSCALEECGNAVVDPAEQCDDGANGNNDDGCTDACTTPVCGDTFIQPSLGEQCD
                     AGPNNSDSGMCTGACKVAKCGDGLVWQNVEQCDDGANNGNTKACKADCTVNVCGDGFK
                     GPGEACDDGNQNNNDACTNVCKLPTCGDGFKTPAEQCDLGANNSNTGACTLACLLPVC
                     GDGFTQPSKGETCDDANNVNTDACVACKLAKCGDNFIQAGVEQCDGNKPGDPCNANCT
                     EPKLVYATSQTYNGNLGGIAGANTKCQILATAAGLEGTYRAWISTENGSPSTNFTKAN
                     TGYLRVDLIKVADDWADLTDGSLDNPIQKTELNTVPGSTDTWTCTTVAGNHVNTLEDC
                     SNWTAGLFGIGREGRTNQINGDWTNTGLDADSLCSTNQRLFCFEQ"
     gene            6868..8679
                     /locus_tag="VGB85_04475"
     CDS             6868..8679
                     /locus_tag="VGB85_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133305.1"
                     /translation="MKSLTNSKAPLPGFALILCGLAGVLAACGDEPGKCEVESGCEEP
                     LDCEAPPDVKLIQLRGPEAEQTMRELCERYPRGVTGALFVEETGLKALELPCLCSLSG
                     SLLIRDNPKLVELGGLAHIEHLEGSLTIVDNPALRTLVGLPKRATLDPAYPTSLEIRN
                     NDALRDLTGLPIFTDAPGPVTITNNDALERLTGLPPSLPHVGGLAIAGNPALVSLAEL
                     PRDLATVDGTLRISVSQGDASELPRTLTRVGKHLHLNGFRSFQEFPPIAEVGEDLSIL
                     GNAVTDLTGFPDGLVVGGGLTIANNPLLDDLAGLPRDLTVGGSLKIEWNDGLVDLSGL
                     PDGLQIGVDEQTGISIAIDGNDQLERLTGLPESLTAVPGTVLLRDNPRLRDLGGLFDH
                     LRAVGGELMVVGNQDLRDLGGIPEGTTLGVGIFGHTLRVEGNERLERLTGLPQSLTWL
                     PGTLSVVDNPALVDLTGLEGIEGIDGDLLIGRRTSDDLGNPYDWSFVPAGNLGLQSLA
                     GLSGLTEVGGILGVTCNPGLVDLASLGQLASVGYLLLLDNPELAGLGELGGPGGALHS
                     VGSYQIRCSPKLDPAAAAAVLAEVAESVPTSIELMCE"
     gene            complement(8717..9484)
                     /locus_tag="VGB85_04480"
     CDS             complement(8717..9484)
                     /locus_tag="VGB85_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HEY0133306.1"
                     /translation="MRVAAIQSDIVWEDPEANFERLRPWVAAAAAAGARLVVLPEMFA
                     CGFSMATAHVQEPPDGPSARFLTEMAKTHGVWICGSLPEVPEGAAKPYNTLVLASPQG
                     SALHRYHKIHPFSFAREHEHYATGEGHVTVDVEGLRCTLFICYDLRFADEFWGRADAT
                     DAYIVVANWPERRRRHWTTLLQARAIENQAYVVGVNRVGHGSGLDYSGDSRIIDPWGE
                     ILASAAGGETMLLADLHPDNVKDAREKFPVLKDRRRV"
     gene            complement(9521..10687)
                     /locus_tag="VGB85_04485"
     CDS             complement(9521..10687)
                     /locus_tag="VGB85_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HEY0133307.1"
                     /translation="MSSRQPHLSARLQGFGTTVFTEMTRLALQHGAVNLGQGFPNFDG
                     PEFVKEAAIAAIKAGHNQYARMFGIPDLNRAIAEHQRRFYGLNYDPDTEITVYCGATE
                     AICATFQALCDTGDEVVIFEPFYDSYRASITMAGAVPRVVTLRAPDFSYRPEELEAAI
                     TPKTRAILLNSPHNPTGKVFSRSELEHIAALCHRHDLIAVTDEVYEHLVYEGTHVPLA
                     TLPGMQERTVLISSSGKTFSFTGWKVGHTCAPPAITAALRCAHQFVTFCTAPPFQHAL
                     ARAFVADDSFFTDLTRDYLRRRDRLCDGLREIGLGVLRPAGTYFVMTDIRPLGYDDDL
                     AFCRMLPEKIGVAAIPPSSFYVNPHEGRHLVRWAFCKTDDVLDEAIKRLQKLRA"
BASE COUNT         1477 a         3463 c         4034 g         1819 t
ORIGIN      
        1 gcggcgcagg agtcactgca accatcttcg gggtcgaggt tgccgtcgtc gcactcctcc
       61 gcgaccgggc cgaagacctc gagcatgccg ttgccgcact gcgccgtcgg ctcgccgctg
      121 ctgccgctgg tggtctcgtc ggtcgtgctc gtgcttgtgc tcgtgctgtc cgggtcgtgc
      181 ggggctgtcg agccgccgct ttcggaggag cctggcgctc cgctcgagcc cgggccctgc
      241 gtcgtgtcgg actcgccggt ctcgctcgcc gtctccgacc agctctcgaa ctcctcgagg
      301 tagcccgcga tccgccgcga cccacatgca ggggcgaccg cgagagatag gaggacacag
      361 gacagtctgg tggtcatttt ttaaaattct cccgagagcg tgaacgcgac ctgttcgcgc
      421 cgcacgtcga gcccgaggtg gatccgtcga cgttgaagtg ttccccggac cagtaatacc
      481 gtgccgaccg tcagcagcgc cgcgccggcc accgcggagc caatgatgat cccctcgtct
      541 cggtgtgtcg ccgccagcag ctcgcgcagt cgcgtgcggt catccacggt gcgggggcgc
      601 gtcgccagct cgtcatggat cgcgtcggcc tcggccgtgc tccggccgtg tcgggacgcg
      661 acgccaccga ccaccgcgaa cgccaccagc ccgagcggca ccattacacc gccggcgatc
      721 tgcatgtgcc tcggggtcgc gtcggtcaca tcggacggcg gcgcgacggc cggtgtgggc
      781 ggcggcgcgg gtggtggagt gacgggcagc agcggggccg gtgggggtgg aggcgcacat
      841 acgtcggggg cgagggcggc gagcagtcgc gtcacctccg cgtggcgctc gcgggcacca
      901 tggatctcca gccggttcgg cgcgtccggg aacgcggccg tcaactgcct gaggtgctcg
      961 tcgaggatgc cctgcaagcg gcacagcacg gcggcctcgc cggtccggtc gtacaggtcg
     1021 agcagggtcc cgcgcagcga gccgagcacc tgttcgcgtc ccgcgaggtc gcggcggggg
     1081 tccggcatcg cctgataggc cgcgtaaaac tcgtcgacgg cgagcgcgag ctcgccggcc
     1141 ttgtaaaaat gcatggcctt cgcgttgtac tcggcggcct cggtggagag cacacccgag
     1201 gcgggagcga gggtcaggag cagcgcgacg cggtgacttg tgaacatcat ggagctcctc
     1261 gccggcggta ggtgtgcacg aaggaggtcg gtgtgccggc cggcagcgtc gccgacgcga
     1321 gcgcggcgct cacacaatcg ccgagaggtg tcgtctccgc gtcgatcacc gtcgcggaga
     1381 tgtgcccgcg tcggtcgacg ctcacctgca ccgggaggcg gggagcgcgg tcgccgtagc
     1441 ggtggaagca tcgctggaca gcatcctcat gtcggctcat gacgcgtcgc gcggccgcca
     1501 cgcggtcgct cgcgtgcgag ggtcgcgtgg ccgcgtggtc ggtcgaggcg acgtccggga
     1561 gcgccagcga agccaggggc acggggacag gcggctggaa tggatcgtcg tggtgtgcgg
     1621 tcggggtgac gttcgccgcg gtctgcgagc aagcgggtgg ctgcgctcgt gaagtcagga
     1681 gataggtcgc cgcgatgcac aggccgcagg cgagcagggc ccagcggacg cgcaccacgc
     1741 gtccggcggg cacggccgtg gcgacgctgg ccgcggcgcg cagggcctcg gcgaacgctc
     1801 gcatcgtctg cgggcgcagg gccgggtctt tttgcagcgc ggactcgacg accgcggtga
     1861 ccgcgggcgg gatgaaggtg gtcccggcgg cgacggtgag ccgtgtcggc tcatgatgga
     1921 tatgcccggc gagcaccgcc gcgatcggct tgccgcgggc ctcgtgaaac gggagccgac
     1981 ctgcgagcac gcgatacagc gtgatcgcca tcgaatagac gtcggagcgg cgatcgacgg
     2041 gtttgccgag cgcctgctcg ggcgaggtat aaccgggcgt gcacagcatg cgaccggcct
     2101 cggtcgtcgg tgggctgtcg gcggtccgca gcgcgcggtc cggcttgacc aggccgaggt
     2161 cgatcaccac cgcgctcggc gggcacccgg cttcacgagt gacgaggatg ttgccgggct
     2221 tgatgtcgcg gtggatgtag ccgcgctcgt ggatcgcgtg cagcgcctcc atcgcgggta
     2281 gcagcagcgc cagcgcctcg tcccacgcga ggcgctggtc ggggcgctcg cgcaggtatt
     2341 gctcgaggtt cgggcccgcg agcagctcca tgacgatgaa cgcggtcgcg tcgagcgagc
     2401 cgctgccgaa cacgcggacc aggttggcgt gacggacctg ggcggcggcg cgggcctcct
     2461 ggaccatgcg cgcgcgcagg tcgtccgagg cgagccgggg cttcaacagc ttgatcgcga
     2521 cgtcgacgcc gagcacctcg tcggtcgcct ggaacacgtc ggccatgcca ccgccgccga
     2581 tctgcgcgcg cacgacgtaa cgaccggcga tcttgcgacc gatgaagtcg tattcgatcg
     2641 ccgagtcggc cgcggggtcg aagtcgccga ccgtgaccag ctcgatggga cttcgctgcg
     2701 cgccggaccg ggacatggga gacctcgtcg gcgctcgaac tcgcgccgcg cggaatcgta
     2761 accccgcgac cccccgctgt cgagggcgtc gccggccgcc tccagcgtca cggcaggcgt
     2821 gtggcgagtg cacgcattgc ccgggcaatg cgtgcactcg gtcgcggtcg gggcggctgc
     2881 ggtgaacagg atgggttgcg gtttttcttc ggggctggtc tgctgaccgg tcatgcctcg
     2941 ccatgcgttg ctcgcggtgg tgctcgggat cgtcgcgggg gcgatgagct gctccgatcg
     3001 ggcggcgccg gacggtacgg aggacggcgg gcacacgagc tcgcaggcga cgagcgcggc
     3061 ggatgctggt gcgccgacga ccggggacgc gggggactcg ggtgggtcga cggcggacga
     3121 ccccgggttg ccggacccgt ccgaggccaa ggcgcacggg ctcggcggcg acccggtgct
     3181 gtgcggcgtg acgctgagcc ccggcggtga cacgctggtc gcggggtcgg tcgcggggcg
     3241 ggctgtgttc ggcgtggagg gagcgctgcg cgtggtcgag gtcggggcga attgctcggc
     3301 ggagctcggg ccgctgctgg cgatcgcggc gaagggcctg cgaatggcgg gcgacggctc
     3361 gctgctggcc gcgcacgagc gcttcgcgct ggtggatttc ggccatcagc gcgatggcat
     3421 gcggttctat ggcctgcccg cggtgacgct gctcggccag caccaggtgg acctgatcag
     3481 cgagtggatc cgcgagatgg acgtcgacga cgctggtacg cgctggtcgc ggatgagcca
     3541 tgacagcgcc gatccgttga tcgacgcggg cgaggtcgcg gggccgtggg agccgccgta
     3601 cctcgggttc ggggtgcagt cgccgaggct cgcgccggat ggcctgcact acgtggtcgg
     3661 gcggtcacgg tactcgttcg aaacttttgc gcggcatacg acgatctatg ggttggacaa
     3721 aaagctcgtc gcgctggagg gggcgccggt cgggttcctc gacaatgaac atatcctgtt
     3781 caatcgctac gacgaggaga gtgacgattt ctccggcgcg gtgatcatcg atctggccgg
     3841 ggatacggtg aatacgaccg agttgcccga gctcaccgag ctgtttcgcc tcgggaccgg
     3901 ggagatcctg gggcggcgca cgcaggacga gcgatgggcg gtcttcgagc cgagcacggg
     3961 cgccgtgtta tggaccgcgg gggagggcgc ccaggtggcg ctggtgggga caaatcagct
     4021 gatcgggctc gtggaggggc gcgtcgagct ggtgcgctgg cggtgatcct gcgcgcggtg
     4081 gaggcgcatg gtgaagggcg tcgccgcccg ttcggccggg ggtgaagccc gcgagtcccg
     4141 ggggactcgc gggctctcgc ggacgtcagc tcgcggcgag agacgagcgc gggcaggtct
     4201 gggcgctcac tgctcgaagc agaacaggcg ctgattggtg ctgcacagcg agtcggcgtc
     4261 gaggccggtg ttggtccagt cgccgttgat ctggttggtc cgaccctcgc gaccgatgcc
     4321 gaacagcccc gcggtccagt tgctgcaatc ttcgagggtg ttgacgtgat taccggccac
     4381 cgtggtgcag gtccaggtgt cggtcgaccc cgggacggtg ttgagctcgg ttttttggat
     4441 cgggttatcg agggagccgt cggtcaagtc ggcccagtcg tccgcgacct taataagatc
     4501 gacgcgcagg tagccggtgt tggccttggt aaaattggtg ctcgggctgc cgttctcggt
     4561 gctgatccag gcgcgatagg tgccctcgag gccggcggcg gtggcgagga tctggcactt
     4621 ggtgttggcg ccggcgatcc cgccgaggtt gccgttgtag gtctggctgg tcgcatacac
     4681 caatttgggc tcggtgcaat tggcgttgca ggggtcgccc ggcttgttgc cgtcgcactg
     4741 ctcgacgccg gcctgaatga aattatcacc gcacttggcg agcttgcagg cgacgcaggc
     4801 gtcggtgttg acgttgttgg cgtcgtcgca ggtctcgccc ttgctcggct gggtgaagcc
     4861 gtcgccgcag accggcagca ggcaggcgag ggtgcacgcg ccggtgttgc tgttgttggc
     4921 gccgaggtca cactgctcgg cgggggtctt gaagccgtcg ccgcaggtcg gaagcttgca
     4981 cacgttggta caggcgtcat tgttgttctg gttgccgtcg tcgcaggcct cgccggggcc
     5041 cttgaagccg tcgccgcaga cgttgacggt gcagtcggcc ttgcacgcct tggtgttgcc
     5101 gttgttggcc ccgtcgtcgc actgctcgac gttttgccag acgaggccat cgccgcactt
     5161 tgcgaccttg caggctccgg tgcacatgcc gctgtcgctg ttgttggggc cggcgtcgca
     5221 ctgctcgccg aggctcggct gtatgaaggt gtcgccgcag acgggggtcg tgcaggcgtc
     5281 ggtgcagccg tcgtcgttgt tgccgttggc gccgtcgtca cactgctcgg cggggtcgac
     5341 gacggcgttg ccgcactcct cgagcgcgca cgaggggctg caaccgtcgg cgccgacgtt
     5401 gttgccgtcg tcgcagccct cgcccgggcc gaggtcgccg tcgccgcaga cgttgaactt
     5461 gcagctggcg aggcagccct ggccggggcc gttgtcgagg ccgttgtcgc actcctcctc
     5521 gccgctgccc tggtcgtgga cgaggccgtc gccgcacacg cccggcacgc aggcgagggt
     5581 gcagtcgccg gcgtcggagt tgtcggggcc ggcgtcgcac tgctcgccga ccggcggctg
     5641 cacgaagccg tcgccgcagg tcgcgttggt gcacgcggag gtgcacaggt cggtgtcgac
     5701 gtcgttgccg tcgtcgcagg cctcctgcgc gttgacgacc gcgtcgccgc aggccgcggc
     5761 gacgcaggcg ttgctgcagt cgtcgttgtc gacgtcgttg ccgtcgtcgc agccctccat
     5821 cggaccgacg atgccgtcgc cgcagaacgc ctgggtgcag tcgctcttgc acgcggcggc
     5881 gtcgtcgttg ttggccccgt tgtcacactc ctcgccgggg ccgatgtcgc cgtcgccgca
     5941 gacgttgccc tggcaattgg cgaggcaggc ctcaccgggg ccgttgttcg ggccgtcgtc
     6001 gcactgctcg ccggggccga cgtcggcgtc gccacagacg ttgagcttgc aatggtcgag
     6061 gcagaagttg ccggggccgt tgttctcgcc gtcgtcgcac tcctcgaagt gcgggtcgaa
     6121 gatgccgtcg ccgcacatcg cctcgaagtc gccggaggtc ccggaggtgc cggaggtgtc
     6181 cgacgcgtcg gtggtgccgg tggtcgtcgt gccgtcggtg gtcgaggtcg aggacgtcgt
     6241 gccgtcggtg gtcgtcgtgc tcgtcgcggt ggtggtggtc gacgtcgcgt cggtggtgct
     6301 cgtggtcgcg tcggtcgtcg atgcggttgg gtcgctcgcc tgcgtcatcg agccctcgcc
     6361 ctgcgtgctc gtcggggtcg tcggctcgct cgcggtcgtc ggcgtcccgg tgctgccgtc
     6421 gggtccagtg ccggtggtgc cggagctgct ctccgaggcg gtctcggtgg tgccggcggt
     6481 gtcgtcggcg cagccgggac aggcgagctg cagcgtcaac agggcgatcg aagtgagcgg
     6541 ggcgaagcgg cggttgtaag acatggcggg cctttcgagg cccgcatgat accactcgcc
     6601 gccatgccga cggccgagcg gtcgcgcgcg cgttcccctt cgctcccgct cgctcgtcgt
     6661 cgccgctcct ctagcaccgg gaagtcttcg ctgcgtcctt gacgctgtca ggatggaggt
     6721 ctgcgagcag catcatgagc tccacagggc tggtggcgca gacatacgtc acgcggatga
     6781 cggagaagat catatcgaag gaggaacatg tagggggctt cggggcgagg tcatcgccgt
     6841 cggatgcgtg tatcattcgc gctgacaatg aagtctctca ccaacagcaa agcgcccctc
     6901 ccaggattcg ccttaatcct ctgtggcttg gccggcgttc tcgctgcttg cggtgacgag
     6961 cctgggaagt gcgaggtgga gtccggatgc gaggagccgc tcgactgcga ggcgcctccg
     7021 gacgtcaagc tgatccagct caggggtcca gaggccgagc agaccatgcg cgagctgtgc
     7081 gagcggtacc cccgtggggt caccggtgcg ctctttgtcg aggagaccgg gctgaaggcg
     7141 ctggagttgc cttgcctgtg ctcgctgagc ggttccctgc tcatccgtga caaccccaag
     7201 ctcgtcgagc tcggtgggct cgcccatatc gagcacctcg aaggatcgct cacgatcgtc
     7261 gacaacccgg cgctcaggac gctcgtcggg ctcccgaagc gcgcgacgct ggatccggcg
     7321 tatcccacga gcctggagat ccgcaacaac gatgcgctgc gcgatctgac cggactcccg
     7381 atattcaccg acgcgccggg gccggtgacg atcaccaaca acgacgcgct cgagcgcctc
     7441 acggggctcc cgcccagcct gcctcacgtc ggcgggctag caattgcggg taatcccgcg
     7501 ctggtcagcc tcgccgagct gccgcgggac ctggccaccg ttgatggcac cctgaggatc
     7561 tcggtatcgc agggcgacgc gagtgagctg ccgcgcacgc tcacgcgggt ggggaagcac
     7621 ctgcacctca acggctttcg cagcttccag gagttcccgc ccatcgccga ggtcggcgag
     7681 gacctcagca tcctcggcaa tgccgtaacc gacctgacgg gcttccccga cggcctcgtg
     7741 gtcggcggtg ggctcactat cgccaacaac ccgctactcg acgacctggc cggcctcccg
     7801 agagacctca cggtcggcgg gtccctgaag atcgagtgga atgacgggct ggtcgacctg
     7861 tccggcctcc cggacgggct gcagatcggc gtcgacgagc agaccggcat cagcatcgcg
     7921 atcgacggca acgaccagct cgagcggctc acgggtctcc cggagagcct caccgccgtg
     7981 cctggcaccg ttctgctgcg cgataacccg cgcctgcgcg accttggcgg attgttcgat
     8041 cacctgcgcg ccgtcggggg cgagctgatg gtggtgggta atcaggacct gcgcgacctg
     8101 gggggcatcc ccgagggcac gaccctcggc gtcggcatct tcggccacac cctccgcgtg
     8161 gagggcaacg agcgcctcga gcggctcacg ggccttccgc agagcctcac gtggctgcca
     8221 ggcacgctca gcgtcgtcga caacccggcg ctggtcgacc tcacgggcct cgaggggatc
     8281 gaagggatcg atggcgatct gctgatcggc cgccgcacca gcgacgacct cggtaatccc
     8341 tacgactggt ccttcgtgcc cgcgggcaac ctcggcctgc agagcctggc ggggctgtcg
     8401 gggctgaccg aggtcggtgg tatcctcggt gtcacctgca accccgggct ggtggatctt
     8461 gcgagtctgg ggcagctggc gtcggtcggc tacttgctgc tgctcgataa ccccgagctg
     8521 gccgggctcg gcgagctcgg cggtcctggc ggcgcgctgc acagcgtcgg cagctatcag
     8581 atccgttgca gcccgaagct cgaccccgcg gctgcggccg cggtgctcgc cgaggtcgcg
     8641 gagagcgtgc ccacgtccat cgaactgatg tgcgagtgag cgcggcgtct acccgatgcg
     8701 ccgagcaagt ccctcgctat acgcggcgcc ggtccttgag caccgggaac ttctcgcgcg
     8761 cgtccttgac gttgtcgggg tggaggtcgg cgagcagcat cgtctcgccg ccggccgcgg
     8821 acgcgaggat ctcgccccag gggtcgatga tgcggctgtc gccgctgtag tcgaggccgc
     8881 tgccgtggcc gacgcggttg acgccgacga cgtaggcctg gttctcgatc gcgcgcgcct
     8941 gcagcagcgt ggtccagtgg cgacggcggc gctcgggcca gttggcgacg acgatgtacg
     9001 cgtcggtcgc gtcggcgcgg ccccagaact cgtcggcgaa gcgcaggtcg tagcagatga
     9061 agagggtgca gcgcaggccc tcgacatcga cggtgacgtg gccctcgccg gtcgcgtagt
     9121 gctcgtgctc gcgggcgaag ctaaacgggt ggatcttgtg atagcggtgc agcgcgctgc
     9181 cctgcgggct ggcgagcacg agcgtgttgt acggcttggc ggcgccctca ggcacctcgg
     9241 gcagcgaccc gcagatccac acgccgtggg tcttcgccat ctcggtaaga aagcgcgcgc
     9301 tcgggccgtc aggcggctcc tgcacgtgcg cggtcgccat cgagaagccg cacgcgaaca
     9361 tctccggcag caccaccaag cgcgcccccg cggccgcggc ggcggcgacc cagggtcgca
     9421 gccgctcgaa gttcgcctcg gggtcctccc acacgatgtc gctctggatc gccgctactc
     9481 gcatcgctta tgtcctttgg ttcaggtgct gatctcgggc tcacgcgcgg agcttttgca
     9541 ggcgcttgat cgcctcgtcg agcacgtcgt cggtcttgca gaaggcccag cgtacgaggt
     9601 gtcggccctc gtgcgggttg acgtagaacg agctcggcgg gatcgcggcg acgccgatct
     9661 tctccggcag catgcggcag aaggcgaggt cgtcgtcgta gccgagcgga cggatgtccg
     9721 tcatcacgaa gtaggtcccg gcgggccgga gcacgccgag gccgatctcc cgtaatccgt
     9781 cgcacagtcg gtcgcgccgg cgcaggtagt cgcgggtcag gtcggtgaag aaggagtcgt
     9841 cggcgacgaa ggcccgggcg agggcgtgct gaaacggcgg ggccgtgcag aaggtcacga
     9901 actgatgtgc acagcgtagc gcagcggtga tcgccggcgg ggcgcaggtg tgtccgacct
     9961 tccagccggt gaagctgaag gtcttgcccg acgaggagat cagcacggtg cgctcctgca
    10021 tgcccggcag ggtcgcgagc ggcacgtgcg tcccctcata aaccaggtgc tcatacacct
    10081 cgtcggtgac ggcgatgagg tcgtggcggt ggcacagcgc ggcgatatgc tcgagctcgc
    10141 tcctcgagaa caccttgccg gtggggttgt gcggcgagtt gaggaggatc gcgcgggtct
    10201 tgggggtgat cgccgcctcg agctcctccg ggcggtagct gaagtcgggg gcgcgcagcg
    10261 tcaccacccg cggcacggcg ccggccatcg tgatgctggc ccggtaagag tcgtagaagg
    10321 gctcgaagat gacgacctcg tcccccgtgt cgcacagggc ctgaaaggtc gcgcagatgg
    10381 cctccgtcgc gccgcagtag acggtgatct cggtgtccgg gtcgtagttg aggccgtaaa
    10441 agcggcgctg gtgctcggcg atcgcgcggt tgaggtcggg gatgccgaac atgcgcgcgt
    10501 actggttgtg cccggccttg atcgccgcga tcgcggcctc cttgacgaat tcggggccgt
    10561 cgaagttcgg gaaaccctgc ccgaggttga cggcgccgtg ctgcagcgcg agacgcgtca
    10621 tctccgtgaa gacggtggtt ccaaagccct gaaggcgggc gctcaggtgc ggttgcctgc
    10681 tgctcatact tcggcgagtg tggcatagtt cggcgcgatg tcggacgccg ccaagcctgc
    10741 cctgcacgac ctcaccgctc acgcgaccca cgtcggggag acgcccgcgc ggc
//
LOCUS       DASWNJ010000247         1878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_640976, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000247 DASWNJ010000000
VERSION     DASWNJ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640976"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VGB85_04490"
     CDS             <1..312
                     /locus_tag="VGB85_04490"
                     /inference="COORDINATES: protein motif:HMM:NF017454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HEY0133308.1"
                     /translation="DRSTPLIYRAMVTGEVLPEVELVVFAPADRTGKTPLYTVKLSNA
                     TISEIRFVTAKDGPEAHEISLTYQKITWTWVEGGVTAEDDWEAPAAKSQVAPKPMPMP
                     R"
     gene            438..908
                     /locus_tag="VGB85_04495"
     CDS             438..908
                     /locus_tag="VGB85_04495"
                     /inference="COORDINATES: protein motif:HMM:NF021082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2383 domain-containing protein"
                     /protein_id="HEY0133309.1"
                     /translation="MPLSKATLDALISQCQGEIAAVDAYDRALKKFAGQPEEPTLAEL
                     RVEHEDSITRLRKLIENDGGKIPHESGAWGSIANAVHTLTAMVNDEVPLQVLQKGEQI
                     GVDGYERALADTELPQEARQELTQLLARCRQHLERLQYLRDHILETPTRPMTAW"
     gene            916..>1878
                     /locus_tag="VGB85_04500"
     CDS             916..>1878
                     /locus_tag="VGB85_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY0133310.1"
                     /translation="MSATVGDFVLERLAAWGIRRIYGYSGDGISGLLEALRAAEGQID
                     LVTTRHEEMAALMACAHAKFTGEVGVCLATSGPGAIHLLNGLYDARMDHQPVVAIVGQ
                     QARSALGSGYQQDVDLVSLFKDVAGAYVQQASEPGQVRHIIDQAIRTARVLRTVTCVI
                     LPKDVQEDAMAQPERAHGSTFSGPGYSEPRVIPTNDDLQRAAQVLNAGERVAMLVGAG
                     ALAATDEVIEIAERLGAGVAKALLGKAAVPDDLPFVTGAIGMLGTKPSTDMMLGCDTL
                     LMVGSSFPYTEFLPKEGQARGVQVDHDGRRLGLRYPMEVNLHGDA"
BASE COUNT          310 a          637 c          673 g          258 t
ORIGIN      
        1 gatcgctcga cgccgctgat ctacagggcg atggtcaccg gggaggtgtt gccggaggtc
       61 gagctggtgg tgttcgcgcc ggcggaccgg accggcaaga cgccgctcta cacggtgaag
      121 ctcagcaacg cgacgatctc ggagatccgc ttcgtcaccg ccaaggatgg gcccgaggcg
      181 cacgagatca gcctgacata tcagaagatc acgtggacct gggtcgaggg tggcgtgacc
      241 gccgaggacg actgggaggc tccggccgcg aagtcgcaag ttgcgcccaa gccgatgccg
      301 atgccgaggt aatgcggcga gggccccggg agacccccgg ggtcggcgtc agcacggccc
      361 cggcgggtcc gccgctgggc ggcttgacag gggttagtcc tcgcccatgg ttgcgtgtct
      421 cccacaagga acctgccatg cctctctcca aagcgaccct ggacgcgctc atctcgcaat
      481 gtcagggtga gatcgcggcc gtcgatgcct acgatcgtgc cctcaagaag ttcgccggac
      541 agcccgagga gcccacgctg gccgagctgc gtgtggagca cgaggactcc atcactcgcc
      601 tgcgcaagct gatcgagaac gacgggggga agatccccca tgaaagcggc gcgtggggca
      661 gcatcgccaa cgcggtgcac acgctgaccg cgatggtcaa cgacgaggtc ccgctgcagg
      721 tcctgcaaaa gggcgagcag atcggcgtcg acggctacga gagggccctc gcggacaccg
      781 agctgcccca ggaggcccgc caggagctga cgcaactgct cgcgcgctgc cgccagcacc
      841 tcgagcgcct gcagtacctg cgggaccaca tcctcgagac cccgacgcgg ccgatgacgg
      901 cgtggtgagg ctctcgtgag cgcgaccgtc ggtgacttcg tgctcgagcg cctcgcggcg
      961 tggggtattc gccggatcta cggctactcg ggcgacggca tctcgggctt gctcgaggcg
     1021 ctgcgcgccg ccgagggcca gatcgatctc gtgacgacgc gccacgagga gatggccgcg
     1081 ttgatggcgt gcgcgcacgc caagttcacc ggcgaggtcg gtgtgtgcct ggcgacctcc
     1141 gggcccggcg cgatccacct gctcaacggc ctctacgacg cgcggatgga ccaccagccg
     1201 gtcgttgcga tcgtcggcca gcaggcccgc agcgcgctcg gcagcgggta tcagcaggat
     1261 gtcgacctcg tctcgctgtt caaggacgtc gccggcgcct acgtgcaaca ggcgagcgag
     1321 ccggggcagg tccggcatat aatcgaccag gcgatccgca cggcacgggt cctgcgcacc
     1381 gtcacctgcg tgatcctccc gaaggacgtg caggaggacg cgatggcgca gccggagcgc
     1441 gcgcacgggt cgaccttctc gggccccggc tacagcgagc cgcgcgtgat ccccaccaac
     1501 gacgacctcc agcgcgcggc gcaggtgctc aacgcgggcg agcgggtggc gatgctcgtc
     1561 ggcgccgggg cgctcgcggc caccgatgag gtgatcgaga tcgcggagcg cctcggggcg
     1621 ggcgtcgcca aggcgctgct cggcaaggcc gccgtgcccg acgacctccc cttcgtcacc
     1681 ggcgcgatcg gcatgctcgg gaccaagccg agcaccgaca tgatgctggg gtgcgacacg
     1741 ctgctgatgg tcggctcgag cttcccgtac accgagttcc tcccgaagga gggccaggcg
     1801 cgcggcgtgc aggtcgacca cgacgggcgc cggctcgggc tgcgctatcc gatggaggtc
     1861 aacctccacg gcgacgcg
//
LOCUS       DASWNJ010000248         2954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_605269, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000248 DASWNJ010000000
VERSION     DASWNJ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605269"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VGB85_04505"
     CDS             <1..264
                     /locus_tag="VGB85_04505"
                     /inference="COORDINATES: protein motif:HMM:NF003757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY0133311.1"
                     /translation="YQRIVGILVKTFELDPADIKPESRLFEELDLDSIDAVDMFVELH
                     EVTGRRINPETARKIRTVQDIVDLVEAEFKANGPAPGSSGSGG"
     gene            272..724
                     /locus_tag="VGB85_04510"
     CDS             272..724
                     /locus_tag="VGB85_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133312.1"
                     /translation="MPYPALHTILPHRPPMILIDELLEYSEHEVVCGVTVREGAPFVS
                     DGRVPALISIEYFAQAVAAFFGYRGRHNPELFAMGMLLGARELDLRSDYFAVGDVLRV
                     AGTLTWSSGELAQFSCELLRGDEVLARAAINVLQTSAGPGGPASPPRD"
     gene            728..1597
                     /locus_tag="VGB85_04515"
     CDS             728..1597
                     /locus_tag="VGB85_04515"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY0133313.1"
                     /translation="MPKLLRILLTGAAFLAFFLAGGFLGRIALPVFKILPGTPERKRQ
                     RRERLLLWAYHLFIRYTNLLGLVEFKAPPLPPELPPPGQAYVLIANHPTLLDTMILLS
                     MFPGLTAVAKYTWYRSWLIRPLLQLGGHIPGPQPALAAGDEAEAQDEGGSTNPALDRM
                     VEHLRAGHPLSIFPEGSRSHERALRRFRRGALEAAIRAQVPIVPVFISVVPPMLMKHQ
                     PWHEVPAQRGRFTVEFFPIIPTAGREIDARALNRELKESYEQRHAQMLAERDALAAAP
                     EKPALPPGRGSHE"
     gene            1642..>2954
                     /locus_tag="VGB85_04520"
     CDS             1642..>2954
                     /locus_tag="VGB85_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0133314.1"
                     /translation="MQTDLRFDVVIGGGGLAGLTLARQLRRELPEARIAVVERQRRPL
                     PAAAHKVGESSVELSAHYFGVVLGLGEYLRTHHFVKNGLRFFPGGGATLPLEQRTEIG
                     PPEFGKVPSFQLDRGRMEDDLRGMIEADGATLLEGFIVKDVELASDAGVDHVVQIEAV
                     DGEHKGEARTLRAGYYVDATGRRALLKTKLGLKRPSGHLANAAWWRVKGRVDLADLVP
                     ASETAWHRRDPEHIRWYSTVHFMGPGYWLWYIPLAPGPEGEAHTSLGVVVHDELHPFD
                     TIRTYERALAWVEKHEPRCHAQIKDIPVEDFLCLRHYSHGSARCFGDRWSLIGDAGVF
                     ADPFYSPGSDFISLANTFTTEILKAHHRGGDPAALVERYDRVYLRTFDATTEVYRKAA
                     PVYGSPQVMAAKVYWDDFTYWSYICQYFFRRLYALPDAEHAEFEA"
BASE COUNT          425 a         1104 c          972 g          453 t
ORIGIN      
        1 tatcagcgga tagtcggcat cctggtcaag accttcgagc tcgaccccgc cgacatcaag
       61 cccgaatcgc gcctcttcga ggagctcgac ctcgacagca tcgacgcggt cgacatgttc
      121 gtcgagctgc acgaggtgac cggccggcgg atcaaccccg agaccgcgcg aaagatccgc
      181 acggtgcaag acatcgtcga tctcgtcgag gccgagttca aggcgaacgg acccgccccg
      241 ggcagttcag gctccggcgg gtgagcggcg cgtgccctac cccgccctgc acacgatcct
      301 gccgcatcgc ccgccgatga tcctcatcga cgagctgctc gagtacagcg agcacgaggt
      361 cgtctgcggc gtgaccgtcc gcgagggcgc gcccttcgtc agcgacggcc gggtgcccgc
      421 gctgatcagc atcgagtact tcgcgcaggc cgtcgccgcg ttcttcggct accgcgggcg
      481 tcacaacccc gagctgttcg ccatgggcat gctgctcggc gcgcgcgagc tcgacctgcg
      541 gtccgactac ttcgccgtcg gcgacgtgct gcgcgtcgcc ggcacgctga cgtggagcag
      601 cggcgagctg gcgcagttct cgtgcgagct gctgcgcggc gacgaggtcc tcgcgcgcgc
      661 cgcgatcaac gtgctgcaga catcggccgg acccggcggg cctgcttcgc ctccccgcga
      721 ctgacacgtg cccaagctcc tgcgcatcct gctgacgggc gccgcgttcc tggccttctt
      781 cctcgctggc ggctttctcg ggcggatcgc cctgccggtc ttcaagatct tgccgggcac
      841 accggagcgc aagcgccagc gacgcgagcg tctgctgctg tgggcctacc acctgttcat
      901 ccgctacacg aacctgctcg ggctcgtcga gttcaaggcc ccgccgctgc cgcccgagct
      961 gccgccgccg ggccaggcct acgtgctgat cgccaaccac ccgacgctgc tcgacacgat
     1021 gatcttgctg tcgatgttcc cgggcctgac cgcggtcgcc aagtacacct ggtaccgctc
     1081 gtggctgatc cgcccgctgc tgcagctcgg cgggcacatc ccggggccac agcctgcgct
     1141 cgcggccggc gacgaggccg aggcgcagga cgagggcggc agcaccaacc cggcgctcga
     1201 tcgcatggtc gagcacctgc gcgccgggca tccgctgtcg atcttccccg agggctcgcg
     1261 ctcgcacgag cgcgcgctgc gacgcttccg ccgcggcgcg ctcgaggcgg cgatccgggc
     1321 ccaggtcccg atcgtgccgg tcttcatctc ggtggtcccg ccgatgttga tgaagcacca
     1381 gccgtggcac gaggtccccg cgcagcgcgg ccgcttcacc gtcgagttct tcccgatcat
     1441 cccgaccgcc ggccgtgaga tcgacgcgcg tgcgctcaat cgcgagctca aggagagcta
     1501 cgagcagcgg cacgcgcaga tgctcgccga gcgcgacgcg ctcgcggcgg cgcccgagaa
     1561 gccggccttg cctccaggcc ggggttctca cgagtgaaca cacgtgcggc gcgccgtcgt
     1621 ttcgctatcc tcgcccccga catgcagacc gatcttcgct tcgacgtcgt catcggcggc
     1681 ggtggactcg cgggactgac actggcccgg cagctgcgcc gcgagctgcc cgaggcgcgg
     1741 atcgccgtcg tcgagcgcca gcgccggccc ctgcccgcgg cggcgcacaa ggtcggcgag
     1801 tcctcggtcg agctctcggc gcactacttc ggcgtcgtgc tcggcctcgg cgagtacctg
     1861 cgcacccacc acttcgtaaa gaacggcctg cgcttcttcc cgggcggcgg cgcgacgctt
     1921 ccgttggaac agcgcaccga gatcgggccg cccgagttcg gcaaggtgcc gtcgttccag
     1981 ctcgaccgcg gccgcatgga ggacgacctg cgcggcatga tcgaggccga cggcgcgacg
     2041 ttgctggaag gcttcatcgt caaggacgtc gagctcgcca gcgacgccgg cgtcgaccac
     2101 gtcgtgcaga tcgaggccgt cgacggcgag cacaagggcg aggcccgcac gctgcgcgcc
     2161 ggctactacg tcgacgcgac cggccgccgc gcgctgctca agaccaagct cgggctcaag
     2221 cggccgagcg ggcacctcgc gaacgcggcg tggtggcgcg tcaagggtcg cgtcgacctg
     2281 gcggacctcg tgcccgccag cgagaccgcg tggcatcgcc gcgaccccga gcacatccgc
     2341 tggtactcga cggtgcactt catgggcccg ggttactggc tgtggtacat cccgctcgcg
     2401 ccgggcccgg agggcgaggc ccacacctcg ctcggcgtgg tcgtgcacga cgagctgcac
     2461 cccttcgaca cgatccgcac ctacgagcgc gcgctcgcct gggtggagaa gcacgagccg
     2521 cgctgccacg cgcagatcaa ggacatcccg gtcgaggact tcctgtgcct gcgccactac
     2581 agccacggct cggcgcgctg cttcggcgat cgctggtcgc tgatcggcga cgccggcgtg
     2641 ttcgccgacc cgttctactc gcccggctcc gacttcatct cgctcgccaa caccttcacc
     2701 accgagatcc tcaaggccca ccaccgcggc ggtgacccgg ccgccttggt cgaacgctac
     2761 gatcgcgtgt acctgcgcac cttcgacgcg acgaccgagg tctaccgcaa ggccgcgccg
     2821 gtgtacggca gcccgcaggt gatggcggcc aaggtctact gggacgattt cacgtactgg
     2881 tcgtacatct gccagtactt ctttcgccgc ctctacgccc tcccggacgc cgagcacgcg
     2941 gagttcgagg cctt
//
LOCUS       DASWNJ010000249         5022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_391800, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000249 DASWNJ010000000
VERSION     DASWNJ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391800"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VGB85_04525"
     CDS             <1..234
                     /locus_tag="VGB85_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133315.1"
                     /translation="ADGTYAPWDERVVPGPAVQTTYKLGQLDLSKANKRGGPKVDVFH
                     GPFLLELDVSIDGRRHKVRSSEFRREDPHLVVT"
     gene            336..2111
                     /locus_tag="VGB85_04530"
     CDS             336..2111
                     /locus_tag="VGB85_04530"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY0133316.1"
                     /translation="MSGRRLVPLLVSLLAPLAGCDRPAPDVTQEQGPAAPILPAPVQD
                     PVATPPAENPETPPPPTGLVLEVPATGEPPPDFGSIKPPLPVPAAAELTVHALAGFEV
                     VAVYSLPDLTSPRLGDQRIGSRTMVTPRIDDRGTGCDNGFHGLPAGGFACASKGLIVD
                     PDKPPYLYMPPPPPRLDAPIPYEYGVISADGTPMWWRIPDAEEVMLAGQRYRAVVAAQ
                     EAAAASAPAKPDSKPAAKPKPKPTADAPAPSETPEPAPVPELPSITDLDAPPPVELTA
                     EEKAEIARREAAAKKRAEARKKEQAEKDRALARKRARLPLNSQTPFMEAGFVVTLGSK
                     VRQQGQQWWKTTRGGFIPANRTYRKETRDFHGGAIPEAGRVAFGFVMSETAAASVRND
                     KGSLQWKRRLDEREFLAFAEEVEVGGRPYLVTPEGLYVRKGDLRLARIATRPPEVQPW
                     ERWIDVDLELQLLVAYEGDAPVYATLVSSGKRGNSEESFLTPTGKFRITAKHISSSMD
                     GNTASDGRYSIQDVPWSMFFHGNYALHGAFWHSKFGERRSHGCVNLGPTDARWLFLWT
                     TPFVPDGWHGVNAHEGAPGSMVVIH"
     gene            2173..3084
                     /locus_tag="VGB85_04535"
     CDS             2173..3084
                     /locus_tag="VGB85_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEY0133317.1"
                     /translation="MSDTPRPSLSQPAIFQPPPEVGLVLVLDLGPDCDPKPGLIAVQK
                     EVAEGDLLVGLGAPLVLALAAEVAGLRGFPALSGMGVAFPSTQGALWLLVGGDERGAV
                     LDRAMDLHGKLGAGWRLLEEVDTFRYRGGRDLTGYEDGTENPKDAAAVEAAVIAEGAP
                     GVRGGSFVAVQRYVHDLARFRGFDAPARDAIIGRHHETNEELAEAAATAHVKRSAQES
                     FDPPAFMLRRSMPWGGVREHGLYFVAFVEALDRFERVLRRMAGLDDGQLDALLGISRA
                     LSGGYYFCPPLAADGTLDLRAVGISTG"
     gene            complement(3092..3808)
                     /locus_tag="VGB85_04540"
     CDS             complement(3092..3808)
                     /locus_tag="VGB85_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133318.1"
                     /translation="MSDPPPAPVIDPREAYIERRLRERNREPEPMLEEEPEEEEDEAD
                     RNDGGPPRGVSLIVPGAVLVGAGFVFTGLGFAVRSLSSGGFCIDNCEENASEAPALSG
                     AVAFTFAALSVAAGTGLLVVGGRRQEEWRAWKARQPPPPPPPSSSDESTPRRGIGMMV
                     SGAAVLVQGTSFSLMVFSADSAVGRTFAGLSAVTGITLLALGARRHVKYKRWRDNQTL
                     VPTAGRTAHGTWVAGVALRF"
     gene            complement(4058..4870)
                     /locus_tag="VGB85_04545"
     CDS             complement(4058..4870)
                     /locus_tag="VGB85_04545"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0133319.1"
                     /translation="MNEVVEDMRMMRWSGASRSSQALPGSPDDSAGSTGEVPALVCSP
                     AGVAGAPAVLFYHGLRASKEVHRKEGRLLAQAGFAAVLIDAPHHGARHDALLEQRDIT
                     GLALDRLLVRLVTDAVAEVPALVDQLLAAGHRSVAIAGISMGAYIALAAAVVEPRLAA
                     IVSLLGSPSWAPEDGVPADMMEAVGRDPLHLHDALVARPLLMINGGKDVNVRPEGARE
                     LAALLRPKYAAAGASEALVHVELPDADHFPGERDWDTLWTTTVGFLTRILDP"
BASE COUNT          715 a         2012 c         1615 g          680 t
ORIGIN      
        1 gccgacggca cctacgcgcc gtgggacgag cgcgtcgtgc ccggcccggc cgtgcagacg
       61 acctacaagc tcggccagct cgacctctcg aaggccaaca agcgcggcgg cccgaaggtc
      121 gacgtcttcc acggcccgtt cctgctcgag ctcgacgtct cgatcgacgg gcgccgccac
      181 aaggtccgct cctcggagtt tcgccgcgaa gacccgcacc tggtggtcac ctgagccggg
      241 cgcccccggg cctacccggg cgtcccggcc ggtcccggac gtttttttgc ggcgacgggt
      301 tgccagcggg cgatcggccg gctcaaagtc gccccgtgtc cggccgtcgc ctcgtccccc
      361 tgctcgtctc cctgctcgcg ccgctcgccg gctgtgatcg gccggcgccg gacgtgacgc
      421 aggaacaggg ccccgccgcg ccgatcttgc ccgcgccggt gcaggacccc gtggcgacgc
      481 cgcccgcgga gaaccccgag acgcctccgc cgccgaccgg cctcgtgctc gaggtccccg
      541 cgaccggcga gccgccgccg gacttcggca gcatcaagcc gccgctgccc gtgccggccg
      601 ccgccgagct gacggtgcac gcgctcgccg gcttcgaggt cgtcgccgtc tactccttgc
      661 ccgacctgac ctcgccgcgg ctcggtgacc agcggatcgg cagccgcacg atggtgacgc
      721 cgaggatcga cgaccgcggc accggctgcg acaacggctt tcacgggctc ccggccggcg
      781 gcttcgcgtg cgcgagcaag ggcctgatcg tcgaccccga caagccgccg tatctgtaca
      841 tgccgccgcc gccgccgagg ctcgacgcgc cgatccccta cgagtacggc gtcatctcgg
      901 ccgacggcac gccgatgtgg tggcggatcc ccgacgccga ggaggtcatg ctcgccggcc
      961 agagatatcg ggccgtcgtc gccgcgcagg aggccgccgc cgcgagcgcg ccggccaagc
     1021 ccgacagcaa gcccgcggcc aaaccaaagc caaagccgac cgccgacgcc cccgcgccgt
     1081 ccgagacccc cgagcccgcg ccggtgcccg agctgccgag catcaccgac ctcgacgccc
     1141 cgccgccggt cgagctgacc gccgaggaga aggcggagat cgccaggcgc gaggccgccg
     1201 cgaagaagcg ggccgaggcg cgcaagaagg agcaggccga gaaggaccgc gccctcgcgc
     1261 gcaagcgggc ccgcctgccc ctcaattccc agaccccgtt catggaggcc ggcttcgtcg
     1321 tcacgctcgg ctccaaggtc cgccagcagg gccagcagtg gtggaagacc acgcgcggcg
     1381 gctttatccc ggccaaccgc acctaccgca aggagacccg cgacttccac ggcggtgcga
     1441 tccccgaggc cgggcgcgtc gccttcggct tcgtcatgtc cgagacggcc gcggcctcgg
     1501 tgcgcaacga caagggctcg ctgcagtgga agcgcaggct cgacgagcgc gagttcctcg
     1561 ccttcgccga ggaggtcgag gtcggcgggc gcccgtacct cgtcacgccc gagggcctgt
     1621 atgtccgcaa gggcgacctc cggctcgccc gcatcgccac gcgcccgccc gaggtccagc
     1681 cgtgggagcg atggatcgac gtcgacctcg agctgcagct cttggtcgcc tacgagggcg
     1741 acgccccggt gtatgcgacc ctcgtctcgt ccggcaagcg cggcaactcc gaggagtcgt
     1801 tcctgacccc gaccggcaag tttcgcatca ccgccaagca catctcgtcc tcaatggacg
     1861 gcaacaccgc ctcggacggc cgctactcga tccaggacgt cccgtggtcg atgttcttcc
     1921 acggcaacta cgccctgcac ggcgcctttt ggcacagcaa gttcggcgag cgccgcagcc
     1981 acggctgcgt caacctcggc ccgaccgacg cccgctggct gttcctgtgg accaccccgt
     2041 ttgtcccgga cggctggcac ggcgtcaacg cccacgaggg cgcccccggc agcatggtcg
     2101 tgattcattg acctcgcacc tgcatgtaca cgcctggcaa agtcagggtc gcatgctacg
     2161 ttcgccgcgc gcatgagtga cacccctcgt ccgtcgcttt ctcagcctgc gatcttccag
     2221 cccccgcccg aggtcggcct cgtcctcgtc ctcgacctcg gcccggactg cgatccgaag
     2281 ccgggcctga tcgccgtgca aaaggaggtc gccgaggggg acctgctcgt cggcctcggc
     2341 gccccgctgg tgctcgcgct cgcggccgag gtcgccggcc tgcgaggctt ccccgccctc
     2401 agcggcatgg gcgtcgcctt cccgtcgacc cagggcgccc tgtggctgct cgtcggcggc
     2461 gacgagcgcg gggcggtgct cgaccgcgcg atggacctgc acggcaagct cggcgccggc
     2521 tggcgcttgc tggaagaggt cgacaccttc cgctaccgcg gcggccgcga cctgaccggc
     2581 tacgaggacg gcaccgagaa ccccaaggac gcggccgccg tcgaggccgc cgtgatcgcc
     2641 gagggcgcgc ccggcgtgcg cggcggcagc ttcgtcgccg tccagcgcta cgtgcacgac
     2701 ctggcccgct ttcgtggctt cgacgccccc gcccgcgacg cgatcatcgg ccgccaccac
     2761 gagaccaacg aggagctcgc cgaggccgcc gcgaccgcgc acgtcaagcg atccgcccag
     2821 gagagcttcg acccgcccgc gttcatgctg cgccgctcga tgccctgggg cggcgtgcgc
     2881 gagcacggcc tgtacttcgt cgccttcgtc gaggccctcg accgcttcga gcgcgtgctg
     2941 cgtcgcatgg ccggcctcga cgacggccag ctcgacgccc tgctcggcat ctcccgcgcg
     3001 ctgtccggcg gctattactt ctgcccgccg ctcgccgccg acggcaccct cgacctgcgc
     3061 gccgtcggca tctccaccgg ctgagcttcg ctcaaaaacg cagcgccacc ccggcgaccc
     3121 aggtcccgtg cgccgtgcgt cctgcggtcg gcaccagggt ctggttgtct ctccatcgct
     3181 tgtacttcac gtgccgccgg gcgccgagcg ccagcaaggt gatacccgtg acggcgctga
     3241 ggcccgcgaa ggtccgcccg accgcgctgt cggccgaaaa caccatcaag gaaaagctcg
     3301 tcccctgcac gaggaccgcc gcgccgctga ccatcatgcc gatgccccgc ctcggcgtcg
     3361 attcgtccga cgatgacggc ggtggcggcg gcggtggctg gcgagccttc cacgcgcgcc
     3421 attcttcctg acgacgcccg ccgacgacga gcagcccggt ccccgcggcg acgctcaagg
     3481 ccgcgaacgt gaaggccacc gcccccgaga gcgcgggcgc ctcactggca ttctcctcac
     3541 agttgtcgat acagaacccg ccggaagaca gcgaccgcac ggcgaatccg aggccggtga
     3601 acacgaagcc cgcaccgacg agcaccgcgc cgggcacgat caggctcaca cctcgcggcg
     3661 gccccccgtc gttcctgtca gcctcgtcct cctcctcctc cggctcctcc tcgagcatcg
     3721 gctccggctc gcggttcctc tctcgcagcc gtcgctcgat gtacgcctct cgcggatcga
     3781 tcaccggcgc cggcggcggg tcggacaccc acggcacctc gccggggacc gcggggtcga
     3841 tctcccgccc ggtcgctggc atctcgggcg tgctcgctcc gacctcgcac ggcgccacgg
     3901 cgaccacgag ccccagcgac gatcccacca tgcgcgtcat cgtgcgcccc accgcgccga
     3961 accatccgtc catcgtcggc agcctgccag aaccggacgc ggcgccctcc gcaatttttg
     4021 ccaccgcggg ccacctgctg cccggacgcg cccggcctca cgggtcgagt atccgcgtca
     4081 ggaatccgac cgtcgtcgtc cacagcgtgt cccagtcgcg ctcgccgggg aagtggtccg
     4141 cgtccggcag ctcgacgtgc acgagcgcct cgctcgcgcc cgccgccgcg tacttcggcc
     4201 gcagcagcgc tgcgagctcc cgcgccccct cgggccgcac gttgacgtcc ttgccgccgt
     4261 ttatcatcag cagcggccgg gccacgagcg cgtcatgtaa gtgcaacggg tcgcggccga
     4321 ccgcctccat catgtccgcc ggcactccgt cctccggcgc ccacgacggc gagccgagca
     4381 gcgacacgat cgccgcgagc ctcggctcga ccaccgcggc cgccagcgca atataggccc
     4441 ccatcgagat ccccgcgatc gccaccgagc ggtgcccggc cgcgagcagc tggtcgacca
     4501 gcgccggcac ctccgcgacc gcgtcggtca ccagccggac cagcaggcga tcgagcgcca
     4561 ggccggtgat gtcgcgctgc tcgagcaacg cgtcgtgcct cgcgccgtgg tgcggcgcgt
     4621 cgatcaacac cgccgcgaac ccggcctgcg cgagcagccg cccctccttg cgatggacct
     4681 ccttgctcgc gcgaaggcca tggtaaaaca gcaccgccgg cgcaccggcg acccccgcgg
     4741 gcgagcacac cagcgccggc acctcgcccg tcgatcctgc cgaatcgtcg ggtgagcctg
     4801 gcaaagcctg cgagctccgc gacgcacccg accaccgcat catgcgcata tcctcgacga
     4861 cttcgttcac gcatcgagct tcgcgcaggc gcccgtgcct gcaaagctcg tggaaacccg
     4921 caccgcgctt cgcggatccc gcgcctttac ttgtgccatg agccgaccca cggtggatcc
     4981 cccctgaatg gtccaatgac ggaacagcac tcgtgagcat at
//
LOCUS       DASWNJ010000250         1995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_110673, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000250 DASWNJ010000000
VERSION     DASWNJ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110673"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="VGB85_04550"
     CDS             complement(<1..1333)
                     /locus_tag="VGB85_04550"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HEY0133320.1"
                     /translation="MHRPSFVSLGVALSIVSGCGDNTGGGSSGAGTTADPGTDATTGS
                     GTVAGEDSTASGAPTGAGTGSGGGPTTGDVTTTDGGTEAGTAGSSETGTDTGVEPGSD
                     LDTALGCPGVYNPDQILDLHLEMAPGDWQTVKGDLSFSIYVEAQFHCGDEAPLTVGVR
                     RKRSGGTHKVGLKVDFNKFVMGQRWQDLRKLSLENGVSSGDPDDDAEVRDLVGEYLGW
                     RVMQRAGVIASRAAFSNLYVNQELIGVYVDVEQVDTRFLKDRFGDNDGWLVKKSGGDG
                     DGPQTHEDDGMADPFAAYFCFWGKGGQACDPPADDVLLAELPGKLDIVQFLRFGAANA
                     YIANTDGPLFKDNNWYYYDWSGGGRAYMPTDLDTTMKSFTNVLTPDGHAFDTVLFTHW
                     MPDYEAILTEIVEVQAPASVMLEELDRVLMVAGPALDGDPWANGTTAAAVAT"
     gene            complement(1420..>1995)
                     /locus_tag="VGB85_04555"
     CDS             complement(1420..>1995)
                     /locus_tag="VGB85_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133321.1"
                     /translation="LLSYVVQRLLGRLAVREVLDFIGMPIYMAINAYSTHVVLREARV
                     IIMGRRLIEELTRRLPEKFAGDDPRELLHATCQFIAVSKRDFHQNHYALTKVLVERYA
                     ITVKTAPSLPEDYLGRLRAADPELRRVCVLVLVLGFILDGKVSWREDRRVATLREAGI
                     VEYSVEEVRGFARAFLSGEGIEPLLNRQLAA"
BASE COUNT          314 a          704 c          653 g          324 t
ORIGIN      
        1 gcgtggcgac ggccgcggcg gtggttccat tcgcccacgg gtcgccatcg agcgcggggc
       61 cggcgaccat cagcacgcgg tcgagctcct cgagcatcac cgacgcgggc gcctggacct
      121 cgacgatctc cgtcaggatc gcctcgtagt cgggcatcca gtgggtgaac agcacggtgt
      181 cgaaggcgtg gccatccggg gtcaacacat tggtgaacga cttcatcgtc gtatcgaggt
      241 ccgtcggcat gtacgcccgc ccgccgccgg accaatcgta gtaataccag ttgttgtcct
      301 tgaacagcgg gccatcggta ttggcgatat acgcgttggc cgccccgaag cgcagaaact
      361 gcacaatgtc gagcttgccg ggcagctcgg ccagcagcac gtcgtccgcc ggcggatcgc
      421 acgcctggcc gccctttccc cagaagcaga agtacgccgc gaaggggtcc gccatgccgt
      481 cgtcctcgtg cgtctgcggc ccgtccccgt ccccgccgct cttcttgacc agccagccgt
      541 cattgtcgcc gaagcgatcc ttcaaaaagc gcgtgtcgac ctgctcgacg tcgacgtaga
      601 cgccgatcag ctcctgattg acgtacaggt tgctaaacgc cgcgcgactg gcgatcacgc
      661 cggcgcgctg catgacgcgc cagccgaggt actcgccgac gaggtcgcgg acctccgcgt
      721 catcgtccgg gtcgcccgag ctgacgccgt tctcgaggct caacttgcgc aggtcctgcc
      781 agcgctggcc catcacgaat ttgttgaagt cgaccttgag gccgaccttg tgggtcccgc
      841 cggagcgctt gcgacggacg ccgaccgtca gcggcgcctc gtcaccgcag tggaattgcg
      901 cctcgacgta gatcgagaag ctcaggtcgc ccttcacggt ctgccagtcg cccggcgcca
      961 tctcgaggtg gaggtcgagg atctggtcgg ggttatagac ccccggacag ccgagcgcgg
     1021 tgtcgaggtc ggacccgggc tccacgccgg tgtcggtccc ggtctcgctg cttcccgcgg
     1081 tccccgcctc ggtcccgccg tcggtcgtcg tcacgtcgcc ggtcgtcggc cccccgccgg
     1141 atcccgtgcc cgctcccgtc ggtgcgcccg acgccgtcga gtcctcgccg gccaccgtgc
     1201 cgctgccggt cgtggcgtcg gtcccgggat ctgcggtggt accggcgccc gacgagccgc
     1261 ccccggtatt gtcgccgcag cccgacacga tcgacagggc gacgccgagg ctcacgaagg
     1321 acggtctgtg catgcagctt ggagcccgca ggcgtccatt tttccaggca cccgcgacga
     1381 ccccgaggtc tgcacgtgcg cccgaccgcg acgggcgtat caggccgcga gctggcgatt
     1441 gagcagcggc tcgatgccct cgccgctgag aaacgcgcga gcgaagcctc gcacctcctc
     1501 gacagaatat tcgacgatcc cggcctcacg cagcgtagcg acccggcgat cctcccgcca
     1561 cgagaccttg ccgtcgagga tgaagccgag caccagcacg agcacgcaga cccggcgcag
     1621 ctccgggtcc gccgcgcgca ggcgcccgag gtaatcctcc ggcaaggacg gggccgtctt
     1681 cacggtgatc gcataacgct cgaccaggac cttggtcagc gcataatgat tttgatgaaa
     1741 gtcgcgcttg ctgacggcga tgaattggca ggtcgcgtgc agcagctcgc gggggtcgtc
     1801 gccggcgaac ttctcaggta accggcgggt cagctcctcg atcaggcgcc ggcccatgat
     1861 gatgacccgg gcctcgcgca ggaccacgtg cgtgctgtag gcgttgatcg ccatgtagat
     1921 cggcatgccg atgaagtcga gcacctcgcg gaccgcgagg cgcccgagca ggcgctggac
     1981 cacgtaactg agcag
//
LOCUS       DASWNJ010000251        16903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_216082, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000251 DASWNJ010000000
VERSION     DASWNJ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 16903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16903
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216082"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VGB85_04560"
     CDS             complement(<1..466)
                     /locus_tag="VGB85_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133322.1"
                     /translation="MPTPHDHKTTSELGPGPSLGTALRVFFKFASPWSFVVALTAVGG
                     LRLWLGNFSVWDLAVFPLILALQPFAEWLIHVYILHFKPKRFLGRVFDFQVAQFHRAH
                     HRDPWHLEDVFIPRRAGFLGLVLLAVGWYLLMPTTELATTALLDSVLMAFVYE"
     gene            complement(450..1205)
                     /locus_tag="VGB85_04565"
     CDS             complement(450..1205)
                     /locus_tag="VGB85_04565"
                     /inference="COORDINATES: protein motif:HMM:NF019456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0133323.1"
                     /translation="MQLKPVARQSLSDAVFEQLSSEIVHGRMVPGAPLPSERSLCDML
                     RVNRGAVREALKRLSQAGLITIQHGGAARVLDYKQSGNLDLLTRLLFHSDGTIDLKVA
                     RSAMEMRAAMAPDIARLAARRRRPGQVHQLLELVAAMSTRPDDLPALQSLALQFWDVL
                     VECSDNIAYQLVFNSLRETYERLRELLVEVLSPELRDLAAYRAIAEAVQREDDLSAAT
                     VARALMELGSSAVFQLLAAIEESPEGEAHADPT"
     gene            complement(1316..2050)
                     /locus_tag="VGB85_04570"
     CDS             complement(1316..2050)
                     /locus_tag="VGB85_04570"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEY0133324.1"
                     /translation="MRWRPRLLPAEPPELLVYDDPTPCPYLEGRQARMPLRVPTRMLS
                     HAEFGQRLRTGDRRQGVLLYRTACPSCRACEPIRIDVEQFAPNKTQRRIYRRGLERLD
                     VEIGPPELTAEKVALYNRHKQGRELSPDGQDIDEQGYRAFLVDTCCDSFEIRYRRDGQ
                     LVGVAIVDRSDDALSAVYFYFDPAFEPLSPGVFSIMKQIELCKTWGLRYLYLGLYIGE
                     CASMAYKATYLPHERLLDGRWQTVEG"
     gene            2088..2909
                     /locus_tag="VGB85_04575"
     CDS             2088..2909
                     /locus_tag="VGB85_04575"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0133325.1"
                     /translation="MHETIAEGVRLLALRTPTLPPATATNTLVVGHRRVAVIEPATPY
                     PDEQAALAELLGSLKEVGGEVVAILLTHHHVDHVGHAEALRERTGAPIHAHPATAARL
                     DFAVDVMMEDGAELDLGEGFALTAMHTPGHAPGHLVFFERRTGLAYAGDMVAGVGTIL
                     IDPEDDGDMTQYLDSLGRIAAAGPKALVPAHGPVQHDPQATLARYRAHRLMREDRILA
                     AIHAAPRDLSAVLADAYADTPSVLWPLAARSLEAHLRKLAAEGRVRRSGDVVALA"
     gene            3094..3501
                     /locus_tag="VGB85_04580"
     CDS             3094..3501
                     /locus_tag="VGB85_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0133326.1"
                     /translation="MPKILIVEDNELSRDMLSRRLRRKGYEVLVATDGQEGIAMAQRE
                     LPDLVLMDLSLPDLDGWEATRRLKKDAKTQHIPVIALTAHAMSGDREKAIDAGCDEYD
                     TKPIDLRRLLSKMIRFVEDPEAAGTGGGEKSMA"
     gene            3889..6675
                     /locus_tag="VGB85_04585"
     CDS             3889..6675
                     /locus_tag="VGB85_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0133327.1"
                     /translation="MIAAIRAQLELRLGYVPALFDPAFISVELLESLWQQTRAAYLDS
                     PLPARFKESLLLAVMRRCPTPYGLVGHAAALARQGVPAAEVLALLEGPGNTPAEARRH
                     LDVLCACTEPWKQWPAAQVLADAVLACSVAYALEPSSNVGVQVELRRLLPVELYTRWA
                     ALMSYLWTRWQWCAAFPELALRTESALSEARARLLAEEPGLAAHLSAADTMELGSGRR
                     SLPTPDALSQIIDAVPTGILQLSIDGAVVMTNAPAQRMFGLTANELSKLTVSDLEQAA
                     VQEDGKPCLPEAYPMTRCLRTLTPQQPTTLGVRRKDGELFWATFTALPLMDPAAQETT
                     GVMVTIVDVSERKRTERALRDSEDKYRVLVEASDAYVLCLDREGRITRANEQASQVLR
                     LPLPALIGKTMHDIISSEELADAHHARYIHVMETGHNLDVEEMIEAEGKTLWFASTVV
                     PLRDHTGEIAGVQVIARDVTSKRRAEIALRDREERSKHEALHDPLTRLPNRLLFMDRL
                     EMEVIHTRQAATESRFAVLCLDLDRFKNINDSLGHSAGDRLLIEFSRRLHECIGPEDT
                     LARLGGDEFAILLRSIHDASDAIRVADRVLNLLKQPFTLRGQDVYVPTSVGIALSASG
                     YERPEDVLRDADTAMYRAKRRGKGRYELFDAGMHAEAVALLTLENDLRRAIERGEFQV
                     YYQPVIDLGNGNITGFEALLRWNHPARGIVLPGEFIGCAEETGLIVPIGEWVLREACN
                     QMRRWHEKYPRSDALTISVNLSGKQFSHPALIGKALRDSALSPSSLKLEITESVIMED
                     PLAASKLLEQLQRQEVQSYVDDFGTGYSSLSYLHRFPMSALKIDRSFVTNIGPGGENA
                     AIVQTIVTLAHNLGMSVIAEGVETAAQLEILRQMGCEYGQGYYFSRAVTASAAEAMIA
                     RQSTLAGTGLLDPA"
     gene            complement(6679..7167)
                     /locus_tag="VGB85_04590"
     CDS             complement(6679..7167)
                     /locus_tag="VGB85_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0133328.1"
                     /translation="MTTARPFRVLGLQQVAVGARDKADLRYLWCELLGLEVVDEFRSE
                     RENVDEDIAILGVGPLQIELDLMQPIDANKKPSVHDPALNHLGLWIDDLAAAVGWLEG
                     RGVRFTPGGVRKGASGHDVCFIHPKGEGPRPHGGVGVLIELVQAPPAVIAAYDAIAAA
                     GG"
     gene            complement(7164..7775)
                     /locus_tag="VGB85_04595"
     CDS             complement(7164..7775)
                     /locus_tag="VGB85_04595"
                     /inference="COORDINATES: protein motif:HMM:NF017939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HEY0133329.1"
                     /translation="MLHLASTTDPRWAAQVPDHLEEILLDHAHCEKKAASTAVSLIFR
                     YPERPALMIPLARLAREELAHFEEVIAALRARGWEFRRLTPSPYATRLMTAVRTHEPA
                     RLLDTLLCCALIEARSCERMQILAETLTDPGLVKLYRGLLACEARHFDSYVDLARSLE
                     LAPEAALMQRLAELAAHEAEVLRETPAEFRLHAATGPLLQSAP"
     gene            7861..8682
                     /locus_tag="VGB85_04600"
     CDS             7861..8682
                     /locus_tag="VGB85_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133330.1"
                     /translation="MPAPLRLAPVLVALTACASNYYAPAPAPTAPDLLAGRPAVQDTP
                     TALLSAQVLPPGPGERHFILPVRAVVHLQFSRPIDPLTLTPQSFVLALADGRRVAPVG
                     AFLSPGTGPGEQRSVALLLAAPPAPTDKDAPVTPPAEPFSLTVTGLLHDAAGRVLEGL
                     AADIRPSSAPVFPVRAEPGPAALCAGRGQALRVFWSGPVARATAAGAPVPVLLRLDGT
                     RGPAELLEATSARPVQDLCAPGMAGVQAVELAAGAMLDERGLPAAGGALTIWPAP"
     gene            8692..9627
                     /locus_tag="VGB85_04605"
     CDS             8692..9627
                     /locus_tag="VGB85_04605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0133331.1"
                     /translation="MARNITRSISLPIVLSSVSVTLSVALLVGWILLIARNKPLAEDF
                     VADTLLLAFGIVSLIVIMTVLVLFSVFLVREIREVRRQTSFIDSVTHELKSPLAALKL
                     CLETLGRADLPPEQNQKLREMMLDDVERLSGFIDRVLAATRLPAERSSLPLQEIDLGA
                     LARRCATVVTRRAHQPEEVVHIDVPPGIVLTSDEVALAVVLENLIDNAIKYSQQDVEV
                     VLSARPGNKGQVVIEVKDRGIGIRRNDLRRVFERFYRVPDEDVRSRRGTGLGLYVVSA
                     LVRSLGGRVAAMSEGPGQGAAMRVTLPTHPRGQRT"
     gene            9803..10549
                     /locus_tag="VGB85_04610"
     CDS             9803..10549
                     /locus_tag="VGB85_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0133332.1"
                     /translation="MTTLSPRLLVVEDEQHLAVGLKLNFELEGYAVDMAPTARKAGEC
                     LLQRPGYDAIILDVMLPDMTGFDLCRKLRDSGNFTPVLMLTALDSTRDRVTGLEVGAD
                     DYLAKPFELTELLARVRSLLRRRSWERQKAPERGGTGPVISFGGAAVDCERQEARMNG
                     QEVKLTRLEFDLVRYFADHAGRVLSRQELQEQVWKLANYPNTRMVDNFIMRLRRHFER
                     DPSAPAHFVSVRGSGYKFVPDPPRAGSEPT"
     gene            10679..11620
                     /locus_tag="VGB85_04615"
     CDS             10679..11620
                     /locus_tag="VGB85_04615"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEY0133333.1"
                     /translation="MSSPMPIPERFRDLPLPPPSQGYDWGPFIPFIALHFLVFGALWT
                     GTTWVDWTVCFALYAIRMFAVTGGYHRYFAHRTYKTSRWFQFLLAFVAQSSSQKGALW
                     WAAHHRNHHKYSDLPWDVHSPIQEGFWYSHVLWLVDRGTEHTDYSKIKDFAKYPELVW
                     LNNHWMVPPVSLGVAVWWLFGWSGLFIGFFLSTVLLWHGTFLVNSLAHVLGKRRFETG
                     DTSRNNWWIALLTLGEGWHNNHHQYMGSARQGFYWWEIDVTFYILKVLSWCGLIWDLR
                     AVPDAILADGRARDAAARRGQTVVRGNSDVLPMTGEY"
     gene            complement(12430..15558)
                     /locus_tag="VGB85_04620"
     CDS             complement(12430..15558)
                     /locus_tag="VGB85_04620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEY0133334.1"
                     /translation="MLGSLIDISIRLRYMMLCLLGVLLVGGVVAARTLPIDAIPDISG
                     IQVSVLTDAPGLSALEVERNVTFPMENALNGVPGMVELRSVSRADISAITIVFADGTD
                     PWFARQLVFERMLQAKNDLPANIPTPQIAPLMTGLGEIYQFVVRSPVHSRKQLRTMLD
                     WEIVPRLRGIPGVIEVNTMGGDLKQFQIVVEPAKLAAFNLTIRELTETLQRSNSVVSG
                     GYLDRGAESFTLRAVGTFAGLEDIGNVVVKTSPGGQPVLVKHVATVRDGAALRHGVIT
                     HNGEDEAVTGVIMMLLGSNSRDVIYAVKDRVEEIQASLPPGVIIETIYDRADFVERTL
                     STVMTNLVEGALVVFIVLVIFLGSIRGALVCVVGIPASMTIALFGMHWAGVTGDLMSL
                     GAIDFGFLVDGPIVVLETLLASYIGKQLTVDERVESYILTIQRVIRPVAFAVAIIMLV
                     YVPLLGLEGVEGRMFRPMATTMAFALLGALIYSVVFLPALLALFVPPLKKDGAGWLRP
                     IGRLYGRSVPYALTLRWPLIAAAAVALVISGYIFKEKGANFLPRIDEGDAVVTIRRAP
                     SVNLEEAKRLDLMVQKTLLEFPEVITTLAMTGRAEVAIDPVGKDTNDTFVRLKPKKEW
                     TTAHDLDGLSVAFKDALERDVPSTFFSISQPIEDRTNEMISGSRADVQIMITGPDLLD
                     LKQISENIRDAVRGIAGTGDVRVERVLGMPELTVKPDRARLARYGVHMEDALLAIEAA
                     RVGMPLGWVYEGNRRFETRLLVPPRAPVPEALGDLLVETPDGPRSPLSEVATITEAEG
                     PAQIRRENRVRIVRVEVNLRGRDLLSWVTDAQQAVQDKIVMPSGYAIQWSGQFENFER
                     ASKRLGMLVPIALALIFGMLLWNFGDIRYAVSVFAVVPFALTGGMAGLILRDMEFSIP
                     AAVGFIALAGVAVLNGVVLATDVRHALAEGGDFDKALIRGSVHTMRAVLTTGAVAALG
                     FLPMALATGAGSEVQRPLATTVVFGIGASTLMTMYLLPAILKITLRREQKAARASVHS
                     TGHSVETLDDHH"
     gene            complement(15577..16809)
                     /locus_tag="VGB85_04625"
     CDS             complement(15577..16809)
                     /locus_tag="VGB85_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0133335.1"
                     /translation="MRMSPFPSRRASGGAAQSAGSDDALAEGMSACLLLAMFALLAML
                     LGVTGCSDNEAAAHDGNEAAPPTLPYFEDGVVRLPPAFAQRIGLKTQDAAITAISPVV
                     YVTGVMEFDEQRIAAVGSRISGRVKEVRVIEGTKVKPGDTLATMESAELGKAQADIFA
                     VKARAQYADTDEERKKKLLSEGIASIRSAELAAQESKITDAELRAAKQRVQALGGSAS
                     GKSLGVMALTSPIEGDVVKVHVFRGQAVEPSYTAFTIADRRSLWVRLSVFEGEVGNIR
                     VGDPVEISAQVTPNEFLTGTVTFISTVLDAANRSAEVRVVVPNEDGKLRVGQAVNARI
                     KPSAASKSALAVPRLAIVQVDGKPTVFVAVDDTGVVPKPIELGVQGADLVEVTSGLNP
                     GDRVVIEGVFALKSELFR"
BASE COUNT         2530 a         5529 c         6249 g         2595 t
ORIGIN      
        1 actcgtagac gaaggccatc aacacgctgt cgagcagggc ggtggtcgcg agctcggtgg
       61 taggcatcaa caggtaccag ccgacggcga gcagcacgag gccgaggaag ccggcgcggc
      121 gcgggatgaa cacgtcctcg aggtgccagg ggtcgcggtg gtgggcgcga tggaactggg
      181 cgacctggaa gtcgaacacg cggccgagga agcgcttggg cttgaagtgc aggatgtaga
      241 cgtggatcag ccactcggcg aagggctgga gcgcgaggat cagcgggaac acggcgaggt
      301 cccacacgct gaagttgccg agccacaggc gcaggccgcc cacggcggtc aaggcgacga
      361 cgaagctcca cgggctcgcg aacttgaaga acacgcgcag ggcggtgccc agcgacgggc
      421 cagggccgag ctcggaggtg gtcttgtggt catgtggggt cggcatgggc ttctccttcg
      481 gggctctcct cgatcgcggc caaaagctgg aagactgcgc tggagccgag ctccatcagc
      541 gcgcgggcga cggtcgcggc ggacaggtcg tcctcccgct ggacggcctc ggcgatcgca
      601 cggtatgccg cgaggtcccg cagctcgggg ctgaggacct cgaccagcag ctcgcgcagg
      661 cgctcgtagg tctcgcgcag agaattgaag acgagctggt aggcgatgtt gtcgctgcac
      721 tcgacgagca cgtcccagaa ctgcagcgcg agcgactgca gggcgggcag gtcgtccggg
      781 cgggtcgaca tcgcggcgac gagctcgagc agctgatgga cttgccccgg gcggcggcgg
      841 cgggcggcga ggcgggcgat gtcgggggcc atcgcggcgc gcatctccat ggcgctgcgg
      901 gcgaccttga ggtcgatcgt gccgtcgctg tggaacagca ggcgggtcag taaatcgagg
      961 ttgccgctct gcttgtagtc gaggacccgg gcagcgccgc cgtgctggat cgtgatcagg
     1021 ccggcctggg acagccgctt gagcgcctcg cggacggcgc cgcggttgac ccgcagcatg
     1081 tcgcacaggc tgcgctcgga cggcagcggc gcgccgggca ccatccgacc gtgaacgatc
     1141 tcgctcgaga gctgctcgaa cacggcatcg gacagggact gacgggcgac gggcttgagc
     1201 tgcatggcga cttatcgagc gtgacatcga gtcagcaggt ggtcaactgg tatgaccagt
     1261 tgcgccagcg tgcgcggaca catcgggggt tctcgccccc tgccctcatc gtggctcagc
     1321 cctcgacggt ctgccagcgg ccgtcgagga ggcgctcgtg cggcaggtag gtggccttgt
     1381 aggccatcga ggcgcactcg ccgatgtaca ggccgaggta gaggtagcgc aggccccagg
     1441 tcttgcagag ctcgatctgc ttcatgatcg agaagacgcc gggggacagc ggctcgaacg
     1501 cggggtcgaa gtagaagtag acggcggaca gggcgtcgtc ggagcggtcg acgatggcga
     1561 cgccgacgag ctggccgtcg cggcggtagc ggatctcgaa ggagtcgcag caggtgtcga
     1621 ccaggaaggc gcggtagccc tgctcgtcga tgtcctggcc gtcggggctg agctcgcggc
     1681 cctgcttgtg gcggttgtag agggcgacct tctcggcggt cagctcgggc gggccgatct
     1741 cgacgtcgag gcgctcgagg ccgcggcggt agatccggcg ctgggtcttg ttgggggcga
     1801 actgctcgac atcgatgcgg atcggctcgc acgcgcggca gctcgggcag gcggtgcggt
     1861 agaggaggac gccctggcgg cggtcacccg tgcgcaggcg ctggccgaac tcggcgtggc
     1921 tgagcatccg ggtcgggacc cgcaggggca tgcgcgcctg gcgaccttcg agataggggc
     1981 acggcgtcgg gtcgtcgtag acgagcagct cggggggctc ggccgggagc agacggggac
     2041 gccagcgcat gccctgcgag tataggccgc gatgctaaag ttgcccggtg cacgagacga
     2101 tcgccgaggg ggtgcggctt ttggcgttgc gaacgccgac cctgccgccg gccaccgcga
     2161 ccaacaccct ggtggtcggg caccggcggg tggcggtgat cgagccggcg acgccctacc
     2221 ccgacgagca ggcggcgctg gctgaattgc tggggagttt gaaggaggtg ggcggcgagg
     2281 tggtggcgat cttgctgacg caccatcacg tcgaccacgt cggccacgcg gaggcgctgc
     2341 gcgagcgcac cggcgcgccg atccacgccc acccggcgac ggcggcgagg ctcgacttcg
     2401 cggtcgacgt gatgatggag gacggggcgg agctcgacct cggcgagggc ttcgcgctga
     2461 cggcgatgca cacgcccggg cacgcgccgg ggcacctggt gttcttcgag cggcggaccg
     2521 gcctggcgta cgcgggcgac atggtcgctg gcgtcggcac gatcctcatc gaccccgagg
     2581 acgacggcga catgacgcag tacctggact cgctcgggcg gatcgcggcg gcggggccca
     2641 aagcgctggt gcccgcgcac gggccggtgc agcacgaccc ccaggcgacc ctcgcccgct
     2701 acagggcgca ccggctcatg cgtgaggacc gcatcctggc ggcgatccac gcggccccgc
     2761 gcgatctctc cgcggtgctc gcggacgcct acgccgacac cccgtcggtg ttgtggcccc
     2821 tggcggcccg ctcgctcgag gctcacctgc gcaagttggc cgcggagggc cgggtacggc
     2881 gcagcggcga cgtggtggcc ctggcgtgag tcgctggccg gggcggctgg ggtcggagca
     2941 tcccaggaat ttgtcggcga ggcgcgggcg cgagcgcgca tcgcggagtc agcgacgatg
     3001 ttcctcgagc aaaaaaaccg taagatggga gccccggtcc ctgggactgt ccatcagcaa
     3061 cctcagtacc gccacggcaa gcgagcccga gccatgccca agatcttgat cgtcgaggac
     3121 aacgaactta gccgcgacat gctgtcccgg cggctgcgtc gcaagggtta tgaagtattg
     3181 gtggcgacgg acgggcaaga gggcatcgcg atggcccagc gcgagctgcc cgacctggtg
     3241 ctgatggacc tgagcttgcc cgacctcgat ggttgggagg cgacgcgccg gctcaagaag
     3301 gacgcgaaga cccagcacat cccagtgatc gcgctgacgg ctcatgcgat gtccggcgac
     3361 cgcgagaagg cgatcgacgc cggctgcgac gagtacgaca ccaagccgat cgacctgcgc
     3421 aggctgctgt cgaagatgat ccgcttcgtc gaggaccccg aggcggcggg caccggcggc
     3481 ggcgaaaagt cgatggcctg agcgccgcgc tcgtgtgctc gtgtgctcgc caggtcgagc
     3541 acgtgtcgat cgtgctctgt ccgtgacgtc ggagtggaac gtgtctcgcc atgagctcga
     3601 tgcgtgctcg tctagtgcca tgcgccggtg tgccagtggt cgcccgcgcc cgcgcctgcg
     3661 cgcctgcgcg cctgcgccgt aaacgtgcgt agaactccgt ccggggggcg ctggcgtgcg
     3721 cagtgtgatt gacacccacg cggcgccgct gtaacgctca atgctggcgc ggattctccg
     3781 cgccatacgc atgggagcgt agcgatctcg gactcatcga cccacggcag cgacgccgtt
     3841 ccagcgtgga acgagccgtc cgcagaggca atccgcaagt cgggtcggat gatcgcggcc
     3901 atccgcgcac agctcgagct gcgcctcggc tatgtgccag ccctgttcga cccggccttc
     3961 atcagcgtcg agctgctgga gtcgttgtgg caacagaccc gcgccgccta cctcgacagc
     4021 cccctgcccg cccgcttcaa ggagtcgctg ctgctggccg tgatgcgccg ctgcccgaca
     4081 ccctacggcc tcgtcggaca cgccgccgcg ctcgcccggc aaggcgtgcc ggcggccgag
     4141 gtgctcgcgc tgctcgaggg ccccggcaac acgcccgcgg aggccaggcg ccacctcgac
     4201 gtgctgtgcg cctgcaccga gccgtggaag caatggccgg cggcgcaggt gctggcggac
     4261 gcggtgctcg cctgctcggt ggcctacgcg ctcgagccga gcagcaacgt cggcgtgcag
     4321 gtcgagctgc gccgactgtt gccggtcgag ctgtacacgc gctgggcggc gctgatgagc
     4381 tacctgtgga cgcgctggca gtggtgcgcg gcgttcccgg agctggcgct gcgcaccgag
     4441 tcggcgctgt ccgaggcgcg cgcgcggctg ttggcggagg agccggggct cgcggcgcac
     4501 ctgagcgcgg cggacacgat ggagctcggc tcgggtcgcc gctcgctgcc gacgccggac
     4561 gccctgagcc agatcatcga cgcggtgccg acgggcatct tgcagctgtc gatcgacggc
     4621 gcggtggtga tgaccaacgc gccggcgcag cggatgttcg ggctgacggc caacgagctc
     4681 tccaagctga cggtcagcga cctcgagcag gcggcggtgc aggaggacgg caagccgtgc
     4741 ctgcccgagg cgtatccgat gacgcgctgc ctgcggacgc tgacgccgca gcagccgacg
     4801 acgctggggg tgcggcgcaa ggacggcgag ctgttctggg cgacctttac ggcgctgccg
     4861 ctgatggacc cggcggccca ggagacgacc ggcgtgatgg tgacgatcgt cgacgtcagc
     4921 gagcgcaagc ggaccgagcg ggcgctccgt gacagcgagg acaagtatcg cgtgctcgtc
     4981 gaggcgagcg acgcgtacgt gctgtgcctc gaccgcgagg gccggatcac ccgcgccaac
     5041 gagcaggcct cgcaggtgct gcggctcccg ctgccggcgc tgatcggcaa gacgatgcac
     5101 gacatcatct cgtcggagga gctggccgac gcgcaccacg ctcgctacat ccacgtgatg
     5161 gagaccgggc acaacctcga cgtcgaggag atgatcgagg ccgagggcaa gacgctgtgg
     5221 ttcgcgtcga cggtcgtgcc gctgcgcgac cacaccggcg agatcgccgg cgtgcaggtg
     5281 atcgcccgcg atgtcacgag caagcgccgc gccgagatcg cgctgcgcga ccgcgaggag
     5341 cgctcgaagc acgaggcgct gcacgacccg ctgacgcgcc tgccgaaccg cctgctgttc
     5401 atggaccgcc tcgagatgga ggtgatccac acgcgccagg cggcgaccga gtcgcgcttc
     5461 gcggtgctgt gcctcgacct cgaccgtttt aaaaatatca acgacagcct cgggcactcg
     5521 gcgggcgacc gcctgctgat cgagttctcg cgccggctgc acgagtgcat cggcccggag
     5581 gacacgctgg cgcgcctcgg cggcgacgag tttgcgatcc tgctgcgcag catccacgac
     5641 gcgagcgacg cgatccgggt cgcggaccgg gtgctcaacc tgctcaagca gccgttcacg
     5701 ctgcgcggcc aggacgtgta cgtgccgacc tccgtcggca tcgcgctgag cgcctcgggc
     5761 tacgagcgcc cggaggacgt gctgcgcgac gcggacaccg cgatgtaccg ggccaagcgg
     5821 cgcggcaagg gccgctacga gctgttcgac gccggcatgc acgcggaggc ggtcgcgctg
     5881 ctgaccctcg agaacgacct gcgccgcgcg atcgagcgcg gcgagttcca ggtctactac
     5941 cagccggtga tcgacctcgg caacggcaac atcaccggct tcgaggcgct gctgcgctgg
     6001 aaccacccgg cgcgcgggat cgtgctgccg ggcgagttca tcggctgcgc ggaggagacc
     6061 ggcctcatcg tgccgatcgg cgagtgggtg ctgcgcgagg cgtgtaatca aatgcgccgc
     6121 tggcacgaga agtacccgcg cagcgacgcg ctgacgatca gcgtcaacct ctccggcaaa
     6181 cagttctcgc acccggccct gatcggcaag gcgctgcgag acagcgcgct gtcaccttcg
     6241 agcctcaagc tggaaattac cgagagcgtg atcatggagg acccgctggc cgcgtcgaag
     6301 ctgctcgagc agctgcaacg ccaggaggtc cagagttatg tggatgactt cggcaccggc
     6361 tacagctccc tgagctacct ccaccgcttc ccgatgtcgg cgctgaagat cgaccggtcg
     6421 ttcgtcacca acatcggccc cggcggcgaa aacgctgcga tcgtgcagac gatcgtgacg
     6481 ctggcccaca acctcgggat gagcgtgatc gccgagggcg tcgagaccgc ggcgcagctc
     6541 gagattttac ggcagatggg ctgcgagtac ggccaggggt actatttttc aagggccgtg
     6601 acggcctcgg ccgcggaggc gatgatcgcc cggcagtcga cgctcgccgg caccgggctg
     6661 ctcgatccgg cgtgaggctc agccgcccgc ggctgcgatc gcgtcgtacg ccgcgatcac
     6721 ggccggcggt gcctggacca gctcgatcag cacccccacc ccgccgtgcg gacgcgggcc
     6781 ctcgcccttg gggtggatga agcagacatc gtgcccggag gcgcccttgc gtacgccgcc
     6841 gggcgtgaag cggacgcccc gtccttcgag ccagccgacg gccgcggcga ggtcgtcgat
     6901 ccacaggccg aggtggttga gggcggggtc gtggacgctg ggcttcttgt tggcgtcgat
     6961 cggctgcatc agatccagct cgatctgcaa cgggccgacg ccgaggatcg cgatgtcctc
     7021 gtcgacgttc tcgcgctcgc tgcgaaactc gtcgacgact tccaggccga gcagctcgca
     7081 ccacaggtag cgcaggtcgg ccttgtcgcg cgcgccgacc gcgacttgtt ggaggccgag
     7141 gacgcggaag gggcgagcgg tggtcatggc gcggactgta acagcgggcc ggtggccgcg
     7201 tgcaggcgga actcggcggg cgtttcgcgc aggacctcgg cctcgtgcgc cgcgagctcg
     7261 gcgaggcgct gcatcagggc ggcctccggc gcgagctcga gggagcgggc gaggtcgacg
     7321 tagctgtcga agtgacgggc ctcgcaggcg agcagaccgc ggtacaactt gacgaggccg
     7381 gggtctgtga gggtctccgc gaggatctgc atgcgctcgc aggagcgagc ttcgatcagg
     7441 gcgcagcaca ggagggtgtc gagcaggcgg gccggctcgt gggtgcggac ggcggtcatc
     7501 aggcgggtcg cgtaggggct cggcgtgagg cgacggaatt cccagccgcg cgcgcgcagg
     7561 gcagcgatca cctcctcgaa gtgggcgagc tcctcgcggg cgagccgcgc cagcgggatc
     7621 atcagggccg ggcgctcggg gtagcggaag atcagcgaga ccgcggtcga ggcggccttc
     7681 ttctcgcagt gcgcgtggtc gaggaggatc tcctcgaggt ggtcggggac ctgcgcggcc
     7741 cagcgcgggt cggtggtcga ggccagatgc agcatgcggc gccggagggt agccgaagcg
     7801 gcgcacgatc gtggcgaggt cgtggtcatc gtcgccggga ggctgctacc ctgccgctcc
     7861 gtgcccgccc cgctccgcct cgcgccggtc ctcgtcgccc tgaccgcgtg cgccagcaac
     7921 tactacgccc cggcccccgc gccgacggcg cccgatctgc tcgcggggcg ccccgcggtc
     7981 caggacacgc cgaccgcgct gctgtcggcc caggtgctgc cgcccgggcc cggagaacgt
     8041 cacttcatct tgccggtgcg cgcggtggtg cacctccagt tctcgcggcc gatcgacccg
     8101 ctgacgctga cgccgcagag cttcgtgctc gcgctcgccg acgggcggcg ggtcgcgccg
     8161 gtcggcgcct tcctgtcccc ggggacaggt cccggcgagc agcgcagcgt cgccctgctg
     8221 ctcgccgcgc cgccggcccc gaccgacaag gacgcccccg tgacgccgcc cgccgagccg
     8281 tttagcctga cggtcacggg gctgctgcac gacgcggccg ggcgggtgct cgagggcctc
     8341 gcggccgaca ttcgcccgtc gagcgcgccg gtgttcccgg tgcgcgccga gcccgggccg
     8401 gcggcgctgt gcgcgggtcg tgggcaggcg ctgcgggtgt tctggagcgg cccggtggcg
     8461 cgtgcgaccg ccgcgggcgc gccggtgccg gtgttgttgc gcctggacgg cacgcgcggg
     8521 ccggcggagc tgctggaggc gacctcggcg cgcccggtgc aggacctgtg cgcgccgggg
     8581 atggccggcg tgcaggcggt cgagctcgcg gccggggcga tgctcgacga gcgcggcttg
     8641 ccggcggcgg gcggcgccct gacgatctgg ccggcaccct gagggacgag catggcgcgc
     8701 aacatcactc gatcgatctc cctgccgatc gtcctctcgt cggtgtcggt gacgctgagc
     8761 gtcgcgctgc tggtcgggtg gatcctgctg atcgcgcgca acaagccgct cgcggaggac
     8821 ttcgtcgccg acacgctgct gctcgcgttc ggcatcgtct cgctgatcgt catcatgacg
     8881 gtgctcgtgc tgttctcggt gttcctggtg cgggagatcc gggaggtccg gcgccagacc
     8941 agctttatcg actccgtcac ccacgagctc aagagcccgc tcgcggcgct caagctgtgc
     9001 ctggagaccc tcgggcgcgc cgacctgccg ccggagcaga accaaaagct gcgcgagatg
     9061 atgctcgacg acgtcgagcg cctgagcggc ttcatcgacc gcgtgctcgc ggcgacccgc
     9121 ctgccggccg agcgcagctc gctgccgctg caggagatcg acctgggggc gctggcccgc
     9181 cgctgcgcga cggtcgtgac ccgccgcgcg caccagccgg aggaggtcgt gcacatcgac
     9241 gtgccgccgg gcatcgtgct gacctccgac gaggtcgcgc tggcggtcgt gctcgagaac
     9301 ctgatcgaca acgcgatcaa gtactcgcag caagacgtcg aggtcgtgct gtccgcgcgg
     9361 ccaggtaaca agggacaggt cgtcatcgag gtcaaggacc gtgggatcgg gatccggcgt
     9421 aacgacctgc gccgcgtgtt cgagcgcttc taccgggtcc ccgacgagga cgtgcgctcg
     9481 cgccgcggga ccggcctcgg gctctacgtg gtgtcggcgc tggtgagatc cctcggcggg
     9541 cgggtcgccg ccatgtccga ggggcccggc cagggcgccg ccatgcgcgt cactttgccg
     9601 acgcaccccc ggggccagcg cacctgaggc aagaagccgg ttctgcccgg tacatggtgc
     9661 gtccaacgaa ctgcgccttc gggccacgca cccgttgaaa gaaagcgacc tgcatcgaca
     9721 gtaaaaaccc ttatgatggt ggggtcgcga cggttgcgtg ggattggtaa atccctggag
     9781 cgccctcgcc gccaccgcca gtatgaccac gctttcgccc cgccttttgg tcgtcgagga
     9841 cgagcaacac ctcgcggtgg ggttgaagct gaattttgag ctcgagggct acgcggtcga
     9901 catggcaccg accgctcgca aggccggcga gtgcttgctc cagcgccccg gttacgacgc
     9961 gatcatcctc gacgtgatgc tgcccgacat gacgggcttc gatctgtgtc gcaagctgcg
    10021 agacagcggc aacttcacgc cggtgttgat gttgacggcg ctcgactcga cgcgcgaccg
    10081 ggtgacgggg ctcgaggtcg gcgcggacga ctacctcgcc aagcccttcg agctgacgga
    10141 gctgctcgcc cgcgtgcgct cgctgctgcg ccggcgcagc tgggagcgac agaaggcgcc
    10201 ggagcgcggc ggcaccggcc cggtgatctc gttcggcggc gcggcggtcg actgcgagcg
    10261 gcaagaggcg cgcatgaacg gacaggaggt caagctgacc cgcctcgagt tcgacctcgt
    10321 gcgctacttc gccgaccacg ccggccgcgt gctcagccgc caggagctgc aggagcaggt
    10381 gtggaagctg gcgaactacc cgaacacgcg gatggtcgac aacttcatca tgcggctgcg
    10441 tcggcacttc gagcgcgatc cttcggcgcc ggcgcacttc gtatcggtgc gcgggtccgg
    10501 ctacaagttc gtccccgacc cgccccgcgc cggctccgag cccacgtagg gcctctgggg
    10561 ggcccgtttt tgcgtagttt cctgcgcagg taatttcccg gtcgatgaca caacgatgac
    10621 acttggtgac cggtcgatga ctgcgtccga cggggagctt tgcgacattg gcggggccat
    10681 gtcctccccg atgccgatcc ccgagagatt ccgcgatctg cctctgccgc ccccgagcca
    10741 gggttacgac tggggcccgt tcatcccgtt catcgcgctg cacttcctgg tgttcggcgc
    10801 gctgtggacc ggtacgacct gggtcgactg gactgtgtgc ttcgccctgt atgcgatccg
    10861 catgttcgcg gtcaccggcg gttaccaccg ctacttcgcg caccggacct acaagaccag
    10921 ccgctggttc cagttcctgc tcgcgttcgt ggcgcagagc agctcgcaaa agggcgccct
    10981 gtggtgggcc gcgcaccacc gcaaccacca caagtactct gacctcccgt gggacgtgca
    11041 ctcgccgatc caggagggct tttggtactc gcacgtgctc tggctcgtcg accgcggcac
    11101 cgaacacacc gactacagca agatcaaaga cttcgcgaag tacccggagc tcgtgtggct
    11161 caacaaccac tggatggtcc cgcccgtctc cctcggcgtg gccgtgtggt ggctgttcgg
    11221 ctggtccggc ctgttcatcg gcttcttcct gagcaccgtc ctgctgtggc acggcacctt
    11281 cctcgtcaac tcgctcgccc acgtgctcgg caagcgccgc ttcgagaccg gcgacacttc
    11341 tagaaataac tggtggatcg cgctgctcac cctcggcgag ggctggcaca acaaccacca
    11401 ccagtacatg ggctccgcca ggcaggggtt ctattggtgg gagatcgacg tcacgttcta
    11461 tattttgaag gtgctgtcgt ggtgcgggct catctgggac cttcgtgcgg tgcccgatgc
    11521 gatcctcgcg gacgggcggg ctcgtgatgc ggcggcccgg cgcgggcaga ctgtcgtgcg
    11581 tgggaactcc gatgttttgc cgatgactgg tgagtactga gctctcgctg ggtcgcccgc
    11641 ttggcgggcg gccgggcgtg ttcggtgctg gcgtgttcgc gaggcgcggc cgggtgcggc
    11701 aggtgctggc gtgttcggga ggcgcggggc gaggctatct gggccgggga ggggccgaca
    11761 ggctgcgggt ccctgcgacg cgggtcctcc caggggacat gctcgccgcc ccatctgtcg
    11821 cgcatgacaa cgttctgtca ccgactccgc atcgcgtcgc cgaaggttga cggggcgtct
    11881 gcgcgtgacc cctgggtcct cccgctgctt cccccgccag tcggcccctg ctccccgggc
    11941 ggatgctgtc tgcgccccgc ttgtcaccgg atgcggcacc gtcgcgtgat gtcagacctc
    12001 cgctgttttg cgcggctatc gtcagcgtga tgctcgtcgc tgggattgca gagctcgcgt
    12061 gatgtgatgt gatgggatcc gggctcgctg ttgttcgcaa catcgtcgcg ctgctggtcg
    12121 ccgcgtgctc gcgtggcatc gggcctcgct gttccgctgc gccatcatcg gctcgctgct
    12181 gatcactggc atcgcagagc tcgcgtgtga ttgggtctca ctgtgtgcgc ccatcgtcgg
    12241 acctgctcgc gtgcggagct catcgcatga tgtcgccgcg cgggtgggtg cggcctgagc
    12301 cttgctcact gcctcggccg gctcgaggca actgggggcg gcgtacgggc acgcgacggc
    12361 ttcgcgtggc gagtagcggg gcgcagacga tgcgtcggcg gtgcccgagc cgggccgggc
    12421 gcgagcggat cagtggtggt cgtcgagcgt ctcgacgctg tgcccggtgc tgtgcaccga
    12481 cgcgcgagcg gccttctgtt cgcgccgcag cgtgatcttg aggatcgcag gcagcagata
    12541 catcgtcatg agggtcgagg cgccgatgcc gaacacgacg gtggtggcga gcgggcgctg
    12601 gacctcggag ccggcgccgg tggcgagggc catcggcagg aagccgaggg ccgcgacggc
    12661 gcccgtggtc agcacggcgc gcatggtgtg cacggagccc cggatcagcg ctttatcaaa
    12721 gtcgccgccc tcggcgaggg cgtggcggac gtcggtcgcg aggaccacgc cgttgagcac
    12781 cgcgacgccg gcgagggcga tgaagccgac ggcggcaggg atcgaaaatt ccatgtcgcg
    12841 caggatcagg ccggccatgc cgccggtgag ggcgaagggc acgaccgcga acacggagac
    12901 ggcgtagcgg atgtcgccga agttccagag cagcatgccg aagatcagcg cgagcgcgat
    12961 cgggacgagc atgccgaggc gcttgctggc gcgctcgaag ttctcgaatt ggccgctcca
    13021 ctggatcgcg tagcccgagg gcatcacgat cttatcctgg accgcctgct gggcgtcggt
    13081 cacccacgag agcaggtcac ggccgcgcag gttgacctcg acgcggacga tgcggacgcg
    13141 gttctcgcgg cggatctggg ccgggccctc ggcttcggtg atcgtggcga cctccgagag
    13201 cgggctgcgg gggccgtcgg gggtctcgac cagcaggtcg ccgagcgcct cggggaccgg
    13261 cgcgcgcggg gggacgagca gacgggtctc gaagcggcgg ttgccctcgt agacccagcc
    13321 gagcggcatg ccgacgcggg cggcttcgat cgccagcagg gcgtcctcca tgtgcacgcc
    13381 gtagcgggcg aggcgggcgc ggtcgggctt gacggtcagc tccggcatgc cgagcacgcg
    13441 ctcgacgcgg acgtcgccgg tgcccgcgat gccgcgcacg gcgtcacgga tgttctcgct
    13501 gatctgcttg aggtcgagca ggtccgggcc ggtgatcatg atctgcacgt ccgcgcgcga
    13561 accgctgatc atctcgttgg tgcggtcctc gatcggctgg gagatcgaga agaaggtcga
    13621 ggggacgtcg cgctcgagcg cgtccttgaa ggcgacgctg aggccgtcga ggtcgtgggc
    13681 ggtcgtccac tccttcttgg gcttgaggcg cacgaaggtg tcgttggtgt ccttgccgac
    13741 cgggtcgatc gcgacctcgg cgcggcccgt catcgccagg gtggtgatga cctccgggaa
    13801 ctcgagcagc gtcttttgga ccatcaggtc gaggcgcttg gcctcttcca ggttgaccga
    13861 cggggcgcgg cggatggtga cgacggcgtc gccctcgtcg atgcgcggga ggaagttcgc
    13921 gcccttctct ttaaaaatgt agccggagat gaccagcgcg acggcggcgg cggcgatcag
    13981 gggccagcgc agggtcagcg cgtagggcac ggagcggccg tagaggcggc cgatcgggcg
    14041 cagccagccc gcgccgtcct ttttgagcgg cgggacgaac agcgcgagca gggccggcag
    14101 gaacacgacc gagtagatca gcgcgccgag cagcgcgaac gccatcgtcg tcgccatcgg
    14161 ccggaacatg cggccctcga cgccctcgag gccgagcagc gggacgtaga cgagcatgat
    14221 gatcgcgacc gcgaaggcga ccgggcggat cacgcgctgg atcgtgagga tgtacgactc
    14281 gacgcgctcg tcgacggtca gctgtttgcc gatgtagctg gccagcaggg tctctagcac
    14341 gacgatcggg ccgtcgacca ggaagccgaa gtcgatcgcg ccgaggctca tcaggtcgcc
    14401 ggtgacgccg gcccagtgca tgccgaacag ggcgatcgtc atcgaggcgg ggatgccgac
    14461 cacgcagacg agggcgccgc ggatgctgcc gaggaagatg accagcacga tgaagacgac
    14521 gagcgcgccc tcgacgaggt tggtcatgac ggtcgacagg gtgcgctcga cgaagtcggc
    14581 gcggtcgtag atcgtctcga tgatgacgcc gggcggcagt gaggcctgga tctcctcgac
    14641 gcggtccttg acggcgtaga tgacgtcgcg gctgttggag ccgagcagca tcatgatgac
    14701 gccggtgacg gcctcgtcct cgccgttgtg ggtgatcacg ccgtggcgca gggccgcgcc
    14761 gtcacgcacc gtggcgacgt gcttgacgag caccggctgg ccgccggggg acgtcttgac
    14821 gacgacgttg ccgatatcct cgaggccggc gaaggtgccg acggcgcgca gggtgaacga
    14881 ctcggcgccg cggtcgaggt agccgccgga gacgacgctg ttggatcgct ggagggtctc
    14941 ggtgagctcg cggatcgtca ggttgaacgc ggccaatttg gcgggctcga cgacgatctg
    15001 gaactgcttg aggtcgccac ccatggtgtt gacctcgatg acgccgggga tgccacgcag
    15061 gcgcggcacg atctcccagt cgagcatcgt gcgcagctgc ttgcgcgagt gcaccggcga
    15121 gcgcacgacg aactggtaga tctcgccgag gccggtcatc agcggggcga tctgcggggt
    15181 cgggatgttg gcgggcaggt cgttcttcgc ctgcaacatg cgctcgaaca ccagctgtct
    15241 ggcgaaccac gggtcggtgc cgtcggcgaa cacgatcgtg atcgcggaga tgtccgcgcg
    15301 cgagaccgag cgcagctcga ccatgcccgg aacgccgttt agagcgttct ccatggggaa
    15361 cgtgacgttg cgttcgacct cgagggccga caggccgggg gcgtcggtca gcaccgacac
    15421 ctggatgccg gagatgtccg ggatggcgtc gatcggcagg gtgcgggcag cgacgacgcc
    15481 gccgacgagc agcacgccga gcaggcacag catcatgtac cgcaggcgaa tggagatatc
    15541 gatcagcgag ccgagcatgg tgagaccccg cgccgcctag cggaacagct ctgacttgag
    15601 ggcgaacaca ccctcgatga cgaccctgtc accggggttg aggccgctgg tgacctcgac
    15661 gaggtcggcg ccctggaccc cgagctcgat cggcttgggc acgacgccgg tgtcgtcgac
    15721 cgcgacgaac acggtcggct tgccgtcgac ctgcacgatc gccaggcgcg gcacggcgag
    15781 cgcgctcttg ctggccgcgc tcggcttgat gcgggcgttg acggcctgcc ctacgcgcag
    15841 cttgccgtcc tcgttgggta cgacgacgcg gacctcggcg ctgcggttgg cggcgtcgag
    15901 cacggtgctg atgaacgtga cggtgccggt caggaactcg ttgggggtca cctgcgccga
    15961 gatctcgacg gggtcgccga cgcggatgtt gccgacctcg ccctcgaaca cggacaggcg
    16021 gacccacagg gaccggcggt cggcgatcgt gaaggcggtg tagctgggct cgacggcttg
    16081 gccgcggaac acgtgcacct tgacgacgtc gccctcgatc gggctcgtca acgccatgac
    16141 gccgagcgac ttgccgctgg cgctgccgcc gagggcctgg acgcgctgtt tggcggcccg
    16201 cagctcggcg tcggtgatct tcgactcctg ggcggcgagc tcggccgagc ggatcgacgc
    16261 gatgccctcc gagagcagct tctttttgcg ctcctcgtcg gtgtcggcgt actgggctcg
    16321 cgccttgacg gcgaagatgt cggcctgggc cttgccgagc tcggcgctct ccatggtcgc
    16381 cagcgtgtcg ccgggcttga ccttggtgcc ctcgatgacg cgcacttcct tgacgcgccc
    16441 ggagatccgc gagccgaccg cggcgatccg ctgctcgtcg aactccatca cgccggtgac
    16501 gtacacgacc ggagagatgg cggtgatcgc ggcgtcctgg gtcttgaggc cgatgcgctg
    16561 ggcgaaggcc ggaggcaggc gcacgacgcc gtcctcgaag tacggcagcg tcggcggggc
    16621 ggcctcgttg ccgtcgtgcg cggcggcctc gttgtcggag cagccggtga cgcccaacag
    16681 catcgccagc agcgcgaaca tcgcgagcag cagacacgcg gacatgccct ctgcgagcgc
    16741 gtcgtcgctg ccggcgctct gggctgcgcc gccgctggcg cgacgggatg gaaacggact
    16801 cattcgcatg gcgtacatcg tgatgaagcg agctggcgag cgccggactg acgcacgctc
    16861 gcagcatacg acatgaacgt ccggctttcg tcgcagcgga gac
//
LOCUS       DASWNJ010000252         2385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_5018, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000252 DASWNJ010000000
VERSION     DASWNJ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5018"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VGB85_04630"
     CDS             complement(<1..337)
                     /locus_tag="VGB85_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133336.1"
                     /translation="MSNKPAITTDKAPAKLIQKPPHKKRGIWDKLKDAVDPDRALQAS
                     MAALIEATAVKHVLGLDEERWAPGKPLKLLLAGYVGTRNTGADVRVEEMIRQIRHVVG
                     DDNLELATMT"
     gene            complement(352..1497)
                     /locus_tag="VGB85_04635"
     CDS             complement(352..1497)
                     /locus_tag="VGB85_04635"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0133337.1"
                     /translation="MAIFLTGATGYIGSYVAAGLLERHPNERLALLVRAKNPVEAEER
                     LWKSMQLHMDFERFLELVRTRCDLYLGDLTLPGLGLTPDARDQLVKSMDSVLHVAASL
                     NRKSSKACFNVNLRGTLAVLKLARDAQDHHGLRRFTDISTVAVCGERQDEVVREDNII
                     DWDRSDYDPYARTKKFCEHMLHELLPDVPGVVLRPSIVLGDSRFPETTQFDMVRAFVM
                     LAQMRVLPFKPDWRLDIVPADYVSKAIVHIHQVERPKYDSYNLSSGTSSLTFREIVEN
                     LEAQGHPVRHRFTPWLNGAFTRTVEAASNTPRSWGLSLPASLLKVFLPYLTFNTVFDN
                     RRVVEELGEAPRPFNEYALGLLRFARDGNFHYPYLPWPGGAQVRQVA"
     gene            complement(1497..>2385)
                     /locus_tag="VGB85_04640"
     CDS             complement(1497..>2385)
                     /locus_tag="VGB85_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133338.1"
                     /translation="PFPGWNQGFNTCTPLIYLLGGWFRHLPARIGNPFDVIPVDYVCR
                     GLVVAGAALLRGTHSPVYQCGSSDRNLLTIDRAIELTALAHRKHLRYHGDTALERVVL
                     SRWDAITTDIDHLMSVPNIRSAAEGIRDWLREVKAPKFMRKQLDEWADKTDDALDTVE
                     TIDKMVDLYQPFVHDNHYIFVSQALFTAEIVEEDFRCDPRSISWRSYWIDIHVPGLRK
                     WCFPLFENKTPPGYTPPHPFKLPRTGTPATPASEPTRLEPRAAHPESGRSPVAASAAS
                     SASSSSSTSSSSASRAEVL"
BASE COUNT          361 a          678 c          952 g          394 t
ORIGIN      
        1 aggtcatcgt cgccagctcg aggttgtcgt cgccgacgac gtggcggatc tggcggatca
       61 tctcctcgac gcggacgtcg gcgccggtgt tgcgcgtgcc gacgtagccg gcgagcagca
      121 gcttgagcgg cttgcccggg gcccagcgct cctcgtcgag gccgagcacg tgcttgacgg
      181 cggtcgcctc gatcagcgcg gccatgctcg cctgtagcgc gcggtcgggg tcgacggcgt
      241 ccttgagctt gtcccagatg ccgcgcttct tgtgcggagg cttctggatc agcttcgcag
      301 gggccttgtc ggtcgtgatg gctggtttgt tcgacatctc aggcgaccat ttcaggcaac
      361 ttgtcggacc tgcgcgccgc cgggccaggg caggtagggg tagtgaaaat tgccgtcgcg
      421 ggcgaagcgg agcaggccga gcgcgtactc gttgaacggg cgcggggcct cgccgagctc
      481 ctcgacgacg cggcggttgt cgaacactgt attaaaggtc aggtagggca ggaacacctt
      541 gagcagcgag gcgggcaggc tcaggcccca gctgcgcggg gtgttggagg cggcctcgac
      601 ggtccgggtg aaggcgccgt tgagccacgg ggtgaagcgg tggcggacgg ggtggccctg
      661 cgcctcgagg ttctcgacga tctcgcggaa ggtcagggag gaggtcccgg acgagagatt
      721 gtacgagtcg tacttgggcc gctcgacctg gtggatgtgg acgatcgcct tgctgacgta
      781 gtcggcgggc acgatgtcga ggcgccagtc gggcttgaac ggcagcacgc gcatctgcgc
      841 gagcatgacg aaggcgcgga ccatgtcgaa ctgggtcgtc tccgggaagc ggctgtcgcc
      901 caggacgatg ctcgggcgca ggaccacgcc aggcacgtcc ggcagcagct cgtgcagcat
      961 gtgctcgcag aacttcttgg tccgcgcgta ggggtcgtag tcgctgcggt cccagtcgat
     1021 gatgttgtcc tcgcggacga cctcgtcctg gcgctcgccg cagacggcga cggtcgagat
     1081 gtcggtgaag cggcgcaggc cgtggtggtc ctgggcgtcg cgcgcgagct tgaggaccgc
     1141 gagggtgccg cgcaggttga cgttgaagca ggccttggac gacttgcggt tgagcgaggc
     1201 ggcgacgtgc aggacgctgt ccatgctctt gacgagctgg tcgcgcgcgt cgggcgtcag
     1261 gccgaggccg gggagcgtca ggtcgccgag gtagaggtcg cagcgggtgc gcacgagctc
     1321 gaggaagcgc tcgaagtcca tgtgcagctg catggacttc cacagccgct cctcggcctc
     1381 gaccgggttc ttggcacgca cgagcagggc gaggcgctcg ttggggtggc gctcgagcag
     1441 gccggcggcc acgtaggagc cgatgtagcc ggtcgcgccg gtgaggaaga tggccattac
     1501 aggacctccg cgcgtgaggc ggacgaagat gaagtggaag acgaggaaga tgcagaagaa
     1561 gcagcagagg ccgcgacggg gctgcggccg gactcggggt gcgcggcgcg gggctcgagg
     1621 cgggtcggct cgctggccgg ggtcgcgggt gtgccggtgc gcggcagctt gaacgggtgc
     1681 ggcggggtgt agccgggggg ggtcttgttc tcgaacagcg ggaagcacca cttgcgcagg
     1741 ccgggcacgt ggatgtcgat ccagtagctg cgccaggaga tgctacgcgg gtcgcagcgg
     1801 aagtcctcct cgacgatctc ggcggtgaac agggcctgcg agacgaagat gtagtggttg
     1861 tcgtggacga agggctggta gaggtcgacc atcttgtcga tcgtctcgac ggtgtcgagc
     1921 gcgtcgtcgg tcttgtcggc ccactcgtcg agctgcttgc gcatgaactt cggcgccttg
     1981 acctcgcgca gccagtcgcg gatgccctcg gcggcgctgc ggatgttcgg cacggacatc
     2041 aggtgatcga tgtcggtggt gatcgcgtcc cagcgcgaca gcacgacccg ctcgagcgcg
     2101 gtgtcgccgt ggtagcgcag gtgcttgcgg tgggcgaggg cggtcagctc gatcgcgcgg
     2161 tcgatcgtca gcaggttacg gtccgagctg ccgcactggt acaccggcga gtgggtgccg
     2221 cgcagcagcg cggcgccggc gacgacgagg ccgcggcaga cgtagtcgac ggggatgacg
     2281 tcgaaggggt tgccgatgcg cgcgggcagg tggcgaaacc atccgccgag gagatagatc
     2341 agcggggtgc aggtgttgaa gccctggttc cagccgggga agggg
//
LOCUS       DASWNJ010000253         2812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_498743, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000253 DASWNJ010000000
VERSION     DASWNJ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498743"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="VGB85_04645"
     CDS             complement(<1..954)
                     /locus_tag="VGB85_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY0133339.1"
                     /translation="MGKIIGIDLGTTNSVVCVMEGNEPKVIANEEGGRTTPSVVAFEQ
                     NGNVLVGQQARRQAITNPENTVFSVKRFMGMPFDELKAEVARVPYKVQRAPNGDAQIN
                     IRGKAYPPPEISARVLMKLKKAAEDYLNEPVTEAVITVPAYFNDAQRQATKDAGRIAG
                     LEVKRIVNEPTAAALAYGFGKGKTGGAKEQVIAVYDLGGGTFDISILEVAENVVEVIS
                     TNGDTHLGGDDFDDRIVKWLIQEFKKDTGLDVGNDKLVLQRLKDAAEKAKIELSSAPE
                     TEVNLPFLTADATGPKHLQIRLSRAKFEGMVEDLVKRSIDPL"
     gene            complement(1150..>2812)
                     /gene="lon"
                     /locus_tag="VGB85_04650"
     CDS             complement(1150..>2812)
                     /gene="lon"
                     /locus_tag="VGB85_04650"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEY0133340.1"
                     /translation="DDESGDAEEFSKKITEAKMPEEAEKVARKQLDRLKSMQPSSAEY
                     TVTRTYLEWLTDLPWSVSTEDLLDIGVVRDVLNEDHYDLEKVKKRILEYMAVLKLNAS
                     KKGPILCLLGPPGVGKTSLGKSIARAIGRKFVRISLGGVRDEAEIRGHRRTYVGSLPG
                     RIVQALKRAGTNNPVVVLDEIDKLGHDFRGDPGSALLEVLDPEQNWSFSDHYLEVPFD
                     LSKVMFIATANYLDPIPPALKDRLEILRLPGYTRQESLAIARRFLLPKQISEHGLDKE
                     DFKITFADDALLELIDSYTHEAGVRNLEREISSIIRGIAVKVVEGEIKDGITITKEVV
                     PDYLGPQKYLPEMAERTAEPGVATGLAWTPVGGEIMFIEATQMPGKGGLMLTGQLGDV
                     MKESAQAAMSYVKSHLKKLGIEKSTIETNDIHVHVPAGGIPKDGPSAGVAMYSAITSL
                     LTGTCVRPDVAMTGEITLRGLVLPVGGIKEKMLAAHRAGIKRIVLPERNRKDVVDVPE
                     EIRQEMEFFYVKNVDEAAVHIFDRPVVPADAQEDPPVAAPADAAS"
BASE COUNT          385 a          878 c         1000 g          549 t
ORIGIN      
        1 gagcgggtcg atcgaccgct tgacgaggtc ctcgaccatg ccctcgaact tggcgcgcga
       61 caggcggatc tgcaggtgct tcgggccggt cgcgtcagcc gtcaagaacg gcaggttgac
      121 ctcggtctcc ggcgcggacg acagctcgat cttcgccttc tcggcggcgt ccttgaggcg
      181 ctgaaggacc agcttgtcgt tgccgacgtc gaggccggtg tccttcttga actcctggat
      241 cagccacttg acgatgcgat cgtcgaagtc atcgccgccg aggtgcgtgt cgccgttggt
      301 cgagatgacc tcgaccacgt tctccgcgac ctcgaggatc gagatgtcga aggtgccgcc
      361 gccgaggtcg tagaccgcga tgacctgctc cttggctccc ccggtcttgc ccttgccgaa
      421 gccgtaggcg agcgccgcgg cggtcggctc gttgacgatg cgcttgacct cgagaccggc
      481 gatgcgaccg gcgtccttgg tcgcctggcg ctgggcgtcg ttgaagtacg cggggacggt
      541 gatcacggcc tcggtgacgg gctcgttgag gtagtcctcg gccgccttct tgagcttcat
      601 cagcacgcgg gcgctgatct ccggcggggg gtaggccttg ccgcggatgt tgatctgcgc
      661 gtcaccgttg ggggcgcgct gcaccttgta agggacgcgc gcgacttcgg ccttgagctc
      721 gtcgaagggc atgcccatga agcgcttgac cgaaaacacg gtgttctcgg ggttcgtgat
      781 cgcctggcgc cgagcctgct ggccgacgag aacattgccg ttctgctcga aggcgacgac
      841 cgagggagtg gtgcgtccgc cctcttcgtt ggcgatgact ttcggctcgt tgccctccat
      901 gacgcagacg acggagttcg tcgtaccgag gtcgattcca atgatctttc ccatgagttt
      961 acgttgcttc ctttccgatt cggccggcaa gataaggccc gaagtttgcc tgtcaacagc
     1021 ggtccggcac gctgtttgcg gggccgcggc tccgccgggg gcctcacaaa acaccagcgc
     1081 ccaccggggg gtgggcgctg tgggctcaaa agggggccag tcgggcggtt ggcccgggtt
     1141 gtcccgggct caggacgccg cgtcggccgg ggccgcgacg ggggggtctt cctgggcgtc
     1201 cgccggcacg accgggcggt cgaagatgtg cacggcggcc tcgtcgacgt tcttcacgta
     1261 gaagaactcc atctcctgcc ggatctcctc cggcacgtcg acgacgtcct tgcggttgcg
     1321 ctccgggagt acgatgcgct tgatgccggc gcggtgggcc gcgagcatct tctccttgat
     1381 gccgccgacc ggcaacacga ggccgcgcag cgtgatctcg ccggtcatgg cgacgtccgg
     1441 gcggacgcag gtgcccgtca acagcgacgt gatcgccgag tacatcgcca cgcccgcgga
     1501 cgggccgtcc ttggggatcc cgccggcggg cacgtggacg tggatgtcgt tggtctcgat
     1561 cgtcgacttc tcgatgccga gcttcttgag gtggctcttg acgtagctca tcgccgcctg
     1621 ggccgactcc ttcatgacgt cgccgagctg gccggtgagc atcaggccgc ccttaccggg
     1681 catctgggtc gcctcgatga acatgatctc gccgccgacg ggcgtccacg cgaggccggt
     1741 cgccacgccg ggctcggcgg tgcgctcggc catctccggc aggtactttt gcgggccgag
     1801 gtagtccggg accacttcct tggtgatcgt gattccgtcc ttgatctcgc cctcgacgac
     1861 cttgaccgcg atgccgcgga tgatcgagct gatctcgcgc tcgaggttac ggacgccggc
     1921 ctcgtgggtg tagctgtcga tcagctcgag cagggcgtcg tcggcgaagg tgatcttgaa
     1981 gtcctcttta tcgaggccgt gctcgctgat ctgcttgggc agcaggaagc ggcgggcgat
     2041 cgccagcgac tcctgacggg tgtagccggg caggcgcagg atctcgaggc ggtccttgag
     2101 ggccggcggg atcgggtcga ggtagttggc ggtggcgatg aacatcacct tggagaggtc
     2161 gaaggggacc tcgaggtaat ggtcgctaaa cgaccagttc tgctcggggt cgaggacctc
     2221 gagcagcgcc gagccggggt cgccgcggaa gtcgtggccg agcttgtcga tctcgtcgag
     2281 gaccacgacg gggttattgg tgccggcgcg cttgagggcc tggacgatgc ggcccggcag
     2341 cgagccgacg taggtgcggc ggtggccgcg gatctcggcc tcgtcgcgca cgccgccgag
     2401 gctgatgcgc acgaacttgc ggccgatcgc gcgggcgatg ctcttgccga gcgaggtctt
     2461 gccgacgccc ggggggccca ggaggcacag gatcgggccc ttcttgctcg cgttgagctt
     2521 gaggaccgcc atgtactcga ggatgcgctt tttgaccttc tcgaggtcgt agtggtcctc
     2581 gttgagcacg tcgcggacga cgccgatgtc gagcaggtcc tcggtgctga ccgaccacgg
     2641 gaggtcggtg agccactcga ggtaggtgcg cgtgacggtg tactccgcgc tgctcggctg
     2701 catgctcttg aggcggtcga gctgcttgcg ggcgaccttc tcggcctcct cgggcatctt
     2761 ggcctcggtg atcttcttcg agaactcctc ggcgtcgccg gactcgtcgt cc
//
LOCUS       DASWNJ010000254         1831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_145889, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000254 DASWNJ010000000
VERSION     DASWNJ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145889"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VGB85_04655"
     CDS             complement(<1..502)
                     /locus_tag="VGB85_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133341.1"
                     /translation="MTRSIGLLGRLLGAGVFFGVACTGDLGMMTTTTEGDTTDAQCPI
                     GSLGCPCTSGFSCDGGLVCAQKTCVKQDPDETTTGEVTTTEDPTTQGEGTTETTDECD
                     ADGSGAIDPACPSGQPYCVKGDCLDCSQLDCAKNSPSLPLCNPDTGRCAACLCDESAP
                     VCDPVAH"
     gene            complement(661..>1831)
                     /locus_tag="VGB85_04660"
     CDS             complement(661..>1831)
                     /locus_tag="VGB85_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEY0133342.1"
                     /translation="NGVCVGCTAIACGDVSGDTPVCDEASGACVQCTASDDAACGGST
                     PICDVAASTCIGCTEHAQCSDSACQQETGACFASVLFVDKGADCASADGTKDLPYCEI
                     GEATAKLTAEPTAILVKPSPSPYTQQVQIAAGRTVAIVRNGSGTARIEVSGTDAVFVS
                     DGATLRLESLQISKGEVTKGLFCTGAKVTLDRTQVTDRKGLGIEAVDCELTLRGSRVY
                     LNLGGGIKLNGGQLRLENSFVVTNGGSFTAISGIVASNGATVDALYSTIADNDGKAGV
                     EDSIDCTNAGAVALRNAIVFGQSAATSVDCAGATATTSVIDSMMLTGEGNVTVPGLDP
                     AWFVDPETGNFAIKAGAPFEDVAVWLTGDPTGDYDGDARPGSDGAKDYAGADRPK"
BASE COUNT          239 a          660 c          650 g          282 t
ORIGIN      
        1 cgtgcgcgac cgggtcacag accggcgcgg actcgtcaca caggcacgcc gcgcagcgcc
       61 cggtgtcggg gttgcacagc ggcagcgagg gcgagttctt ggcgcagtcg agctggctgc
      121 agtcgaggca gtcgcccttg acgcagtacg gctgcccgct cggacaggcc gggtcgatcg
      181 cgccggagcc gtcggcgtcg cactcgtcgg tggtctcggt cgtcccctcc ccctgggtgg
      241 tcgggtcctc ggtggtcgtg acctcgccgg tggtcgtctc gtccgggtct tgcttgacgc
      301 aggtcttttg cgcgcacacc aggccgccgt cgcacgagaa gcccgaggtg cacgggcagc
      361 cgaggctgcc gatcgggcac tgcgcgtcgg tcgtgtcgcc ctcggtggtg gtggtcatca
      421 tcccgaggtc gcccgtgcac gcgacgccga aaaagacgcc cgccccgagc agtcgaccga
      481 gcagaccgat ggaccgagtc atggtcgggt cagtattgcc tgaaggcccg cgggctgaca
      541 atcggcgcgc cgaaatgcga cggcgcgaag gcgcgccgcg agagcgaccg acaacgccgc
      601 tgcgcgcccc ccggcaccgc cccgatcacc acccccgggt cccggccccg gcggccgcgc
      661 tcacttcggg cggtcggcgc ccgcgtaatc tttggcgccg tcgctgcccg gacgcgcgtc
      721 gccgtcgtaa tcaccggtgg gatcgccggt cagccagacc gcgacgtcct cgaagggggc
      781 gccggccttg atcgcgaagt tccccgtctc gggatcgacg aaccacgcgg ggtcgaggcc
      841 ggggaccgtc acgttgccct cgccggtcag catcatcgag tcgatcacgc tggtcgtcgc
      901 ggtcgcaccg gcgcagtcga ccgaggtcgc cgcgctctgc ccgaacacga tcgcgttgcg
      961 cagcgcgacg gcgccggcgt tggtgcagtc gatcgaatcc tcgacgccgg ccttgccgtc
     1021 gttgtcggcg atcgtcgagt acagcgcgtc gaccgtcgcg ccgttgctgg cgacgatccc
     1081 ggagatcgcg gtgaacgacc cgccgttggt gacgacgaag ctgttctcga ggcgaagctg
     1141 accgccgttg agcttgatcc cgccgccgag gttgagatac acccgcgacc ctcgcagcgt
     1201 cagctcgcag tcgaccgcct cgatgccgag gcccttgcgg tcggtcacct gggtgcggtc
     1261 gagcgtcacc ttggcgccgg tgcagaacag gcccttggtg acctcgccct tgctgatctg
     1321 cagcgactcg agccgcagcg tcgcgccgtc gctgacgaac accgcgtccg tgccggagac
     1381 ctcgatgcgc gcggtgccgc tgccgttgcg gacgatcgcc acggtgcgcc cggcggcgat
     1441 ctggacctgc tgggtgtagg gcgacgggct cggcttgacg aggatcgcgg tcggctcggc
     1501 ggtcagcttc gcggtcgcct cgccgatctc gcagtacggc aggtccttgg tgccgtcggc
     1561 gctggcgcag tcggccccct tgtcgacgaa caggacgctc gcgaagcacg cgccggtctc
     1621 ctgctggcag gcgctgtcgc tgcactgggc gtgctccgtg cagccgatgc acgtcgaggc
     1681 cgcgacgtcg cagatcggcg tcgaaccgcc gcaggccgcg tcgtcgctcg ccgtgcactg
     1741 cacgcacgcg cccgaggcct cgtcgcagac cggcgtgtcg cccgagacgt cgccgcaggc
     1801 gatcgccgta cagccgacgc acaccccgtt c
//
LOCUS       DASWNJ010000255         1548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_5166, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000255 DASWNJ010000000
VERSION     DASWNJ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5166"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(262..>1548)
                     /locus_tag="VGB85_04665"
     CDS             complement(262..>1548)
                     /locus_tag="VGB85_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY0133343.1"
                     /translation="VELPAGGAPCGAEVSESCREALRSTVKAVREGLLGGGGARWFGA
                     GTSVLPDGATRIIVAVSDRGVQLDPFPAEAALGGKIAFHGRLLGKRSKPSVEVVDAEG
                     KWITLPARVTRNEFRAELTCDRGRGAYQVEVLAEGIYGPEVTANFPVYCGVQAPRTLR
                     VEVEQVGAEVGALDVARSNFAALNNQRTRQGLPALQWDNAAAVVAEAHSQDMLANNFV
                     GHDSPRTGDVEARFRRAGIAMAVLRENVARGYGPRGIHESLMASPGHRVNMLAADVTH
                     VGIGAVIGAPETSAAGAPRPIFLTQNFYAKIGGDLPADPVAALQSRVDELRKAGGLPA
                     ITWDPALFGNSQKLAEGIAAGKKKAAEAAYDATLQALPYRTVQHHEVLAPSFSALDGL
                     KVWQQRNPGVMGLGVAQVTSGENAGSLMVIVTVATR"
BASE COUNT          196 a          595 c          518 g          239 t
ORIGIN      
        1 gcggatctgg ccgttgttgc cgtgcacgag cacctcgctc tgctgatgac gggccagctc
       61 ggtggcccgc tcgacggcct cctcctgggt gctgtggacc gacgaggcgc gctcgccatc
      121 ctcgggcttg acggcccaac cattgttgtg gggaacgacg tgttggttcg gcatgctttg
      181 tctccctggc ctttgggcca gtcgagcgaa acatgatccg ggtccgccgg gctgtcgagc
      241 cggtcggccc cggatcttcg cctaacgcgt cgccaccgtc acgatgacca tcagcgatcc
      301 cgcgttctcg ccgctggtca cctgcgcgac gccgaggccc atgacccccg ggtttcgttg
      361 ctgccacacc ttgagcccgt cgagcgccga gaagctcggc gcgagcacct cgtggtgctg
      421 caccgtccgg tacggcagcg cctggagcgt cgcgtcgtac gccgcctcgg cggccttctt
      481 cttgcccgcg gcgatgccct cggcgagctt ttgcgagttg ccgaacagcg cggggtccca
      541 cgtgatcgcc ggcagcccgc cggccttgcg cagctcgtcg acgcggctct gcagggcggc
      601 gacagggtcc gcgggcagat cgccgccgat cttcgcgtag aagttctgcg tcagaaagat
      661 cggccgcggc gcgccggcgg ccgaggtctc cggcgcgccg atgacggccc cgatgccgac
      721 gtgcgtcacg tccgcagcca gcatgttcac gcggtgcccc gggctcgcca tcaggctctc
      781 gtggatcccc cgcggcccgt agccgcgcgc cacgttctct cgcagcaccg ccatagcgat
      841 gcccgcgcgg cgaaaccggg cctcgacgtc gccggtgcgc ggcgagtcgt ggccgacgaa
      901 gttgttggcg agcatgtcct ggctgtgcgc ctcggccacc accgccgccg cgttgtccca
      961 ctgcagcgcc ggcaggccct ggcgcgtgcg ttgattgttg agcgccgcga agttcgagcg
     1021 cgcgacgtcg agggcgccga cctcggcccc gacctgctcg acctcgacgc gcagcgtccg
     1081 gggcgcctgc acgccgcagt acaccgggaa gttggcggtg acctcgggcc cgtagatgcc
     1141 ctcggcgagc acctccacct gatacgcccc gcgcccgcgg tcgcaggtca gctcggcgcg
     1201 gaactcgttg cgagtgaccc gcgccggcag cgtgatccac ttgccctccg cgtcgaccac
     1261 ctcgaccgag ggcttgctgc gcttgccgag cagccgcccg tgaaacgcga tcttgcctcc
     1321 gagagccgcc tcggcgggga acgggtcgag ctgcaccccg cgatccgaca ccgcgacgat
     1381 gatgcgcgtc gcaccgtccg gcagcaccga cgtgccggcg ccgaaccacc gcgcgccgcc
     1441 tccgccgagc agcccctcac gcaccgcctt caccgtcgag cgcagcgcct cgcgacatga
     1501 ctcgctgacc tcggccccgc acggcgcacc gcccgcgggc agctcgac
//
LOCUS       DASWNJ010000256         1772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_675359, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000256 DASWNJ010000000
VERSION     DASWNJ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675359"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..>1772
                     /locus_tag="VGB85_04670"
     CDS             81..>1772
                     /locus_tag="VGB85_04670"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY0133344.1"
                     /translation="MSSTEEQIRQIHAAIAAQETLRPVLGDAVVEVTLAALRNQLSTL
                     ALPIPGSAPIRTDALLAQLQGYLPRELADKMRAFGRIDSERKQVTVLFADISGFTEMS
                     ERLDAEEVAAMTDAVLQELARAVYESEGYIDKFIGDSVMAVFGAPIVHEDDPDRALRA
                     ALAMREYLKKFNDSWMKRVGAPLTLHIGINTGLVIAGNVGSDLRLSYTVMGDTVNTAS
                     RLENAARPGQILVSRNTYRLTQESFAFVALDPIQVKGKREPLVVYELHHARLTPGKSR
                     GLKEFKPVFVEREREVAALAELARGLQAGRGHLLVITGDAGIGKSRLMAHWRASFAPQ
                     ARCLEGRSFPHTTSLPYGPFRDLFLRYASVGDDDREDVVRLHLRAMIERWFQGDLTAR
                     ALFASMLALHPTPEEDAVLAELPAERRRRELFALVHAWLLALATEGPVILVLEDMHWA
                     DTTSIELVESLLPLVRTHPLIIVGVLRTQRSEAPRVFAPSVTAQYAGHCTLFALTPIS
                     ERGSIGMVQELLQTASLPGSIERIISRKAEGNPFFIEEVVRMLVERGALVRSPGGD"
BASE COUNT          266 a          611 c          612 g          283 t
ORIGIN      
        1 atgatcgcat cggttccctt tcgccgctcc atggtgcgac aatatcaccc gtctcggagg
       61 tctccgggac cagcatacgc atgtcatcca cggaagagca gatccggcag atccacgcgg
      121 ccatcgcggc ccaggagacg ctgcgaccgg tgctcggcga cgccgtcgtc gaggtcacgc
      181 tggcggccct gcgcaaccag ctctcgaccc tcgcgctgcc gatcccgggc tccgccccga
      241 tccgcaccga cgcgctcctg gcccagctgc agggctatct gccgcgggag ctggcggaca
      301 agatgcgagc atttggccgc atcgacagcg agcgcaagca ggtcacggtc ctgttcgccg
      361 atatctccgg tttcaccgag atgagcgagc ggctcgacgc cgaggaggtc gcggcgatga
      421 ccgacgcggt gcttcaggag ctcgcccgcg cggtgtacga gtcggaggga tatatcgaca
      481 agttcatcgg agattcggtg atggccgtgt tcggcgcgcc gatcgtgcac gaggacgacc
      541 ccgaccgcgc gctgcgggcc gcgctggcga tgcgcgagta tctcaagaag ttcaacgaca
      601 gctggatgaa gcgggtcggc gcgcccctga ccctgcatat cgggatcaac accggcctgg
      661 tgatcgcggg caacgtcggc tccgatctgc ggctgtcgta tacggtgatg ggggacaccg
      721 tcaacaccgc ctcgcgcctc gagaacgcgg cgcggccggg gcagatcctc gtcagccgaa
      781 acacctatcg cctgacccag gagtcgttcg cgtttgtcgc cctcgacccg atccaggtca
      841 aaggcaagcg cgagccgctg gtcgtctacg agttgcatca cgcccgcctg acgcccggca
      901 agtcgcgcgg gctcaaggag ttcaagccgg tgttcgtcga gcgcgagcgt gaagtggccg
      961 cgctcgccga gctcgcccgg ggattacaag ccgggcgcgg ccacctgctg gtgatcaccg
     1021 gcgacgcggg gatcgggaag tcgcgcctga tggcccactg gcgcgcctcc ttcgcccccc
     1081 aggcgcgctg cctcgagggc cggtcgttcc cgcatacgac ctcgctgcct tatggcccgt
     1141 ttcgcgacct gttcctgcgc tacgccagcg tcggcgacga cgaccgcgag gacgtcgtcc
     1201 gcctgcacct gcgcgcgatg atcgagcgat ggttccaggg cgacctgacg gcgcgggcgc
     1261 tgttcgcctc gatgctggcg ctgcacccga cgcccgagga ggacgcggtg ctcgccgagc
     1321 tgccggcgga gcggcgccgt cgtgagctgt tcgcgctcgt gcacgcgtgg ctgctcgcgc
     1381 tcgccacgga ggggccggtc atcctcgtgc tcgaggatat gcactgggcc gatacgacga
     1441 gcatcgagct cgtcgagtcg ttgctgccgc tggtccggac ccacccgctg atcatcgtcg
     1501 gcgtgctgcg gacccagcgc agcgaggcgc cgcgggtgtt cgcgccgagc gtcaccgcgc
     1561 agtacgccgg tcactgcacg ctgtttgcgc tgacgccgat cagcgagcgc gggagcatcg
     1621 gcatggtcca ggagctgctg cagaccgcga gcctgccggg ctcgatcgag cggatcatct
     1681 cccgcaaggc cgagggcaac ccgttcttca tcgaggaggt cgttcgcatg ctggtcgagc
     1741 gcggggcgct cgtgcgctcg cccggcgggg ac
//
LOCUS       DASWNJ010000257         6395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_145949, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000257 DASWNJ010000000
VERSION     DASWNJ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6395
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145949"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1235)
                     /locus_tag="VGB85_04675"
     CDS             complement(<1..1235)
                     /locus_tag="VGB85_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133345.1"
                     /translation="MLVGSAARAGEVPVADVPGLLAAIQAAQPGDTIVLAPGTYEIQQ
                     NKVACEAMGTADAPIVVQAAELGTVLIKFDALEGFHVRGANWEFANLDIEGTCGNDSD
                     CEHAFHVTGAADGTHLHHNKMHGFNAMIKGNGEPIGMGGSYVWPDDVTIEYNELYNDA
                     PRDTGNPVTPVDVVGGHNWIVRGNFIHDHAKGGGDNISYAAFLKGHSKDGIIERNLIV
                     CELLHSGQVRLGLSLGGGGTGPDSICEDGKCKPEHERGIIRNNLIVNCPADVGVYINA
                     GADSKIINNTLYNTSGIDMRFAETTGLVANNILNGKVRDRDGGTSMKMNNLEEVGLDV
                     LGGWFQDPGAYDFTLKDGAMLVDLGLAVPEVTDDYCGNARDDGKHDIGAIEYDGDGCN
                     GGLPVEGGGEGGNTSTSGS"
     gene            1584..3650
                     /locus_tag="VGB85_04680"
     CDS             1584..3650
                     /locus_tag="VGB85_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0133346.1"
                     /translation="MPLLLGLAALAAAFALQAPTAATAAAIAGIAAGVTGWLIASRAE
                     LALGRADEDRLALKEAEGRSRQAERAVQDHGAQVQNLQAQLQSAQQQVQAAQQQIRGA
                     ELQAQEFASRVQALDGENRNLQLQVRTQEVETRTLQARLQDLTTRAHDAEERVRVLES
                     ELRQAQNRGNSRNDARQDAELEALRHEVEEQRRTATETRARLSAELDGMRETLARNEV
                     ELRQAQTRVAEVTSQRDGALRDANQLQTMIDRGLPSGGLDDEATALLHELNEGFTSSA
                     QAIEETSQALTTVNGGLKGIADNVELLASNAEESASSILEMAAANDEVAESMLNLAAS
                     VQETATSIEEMTFSVKEVAKNIEALSSTAEETSAAMNEMDGSIQQVENNANETAQLSE
                     EVVWAAEGGVDAINQTIEVINLIKTYSAEGVTVIGRLGERISEIGKILSVIDDVSEQT
                     NLLALNAAIIAAQAGEHGKGFAVVADEIKDLAERSATSTKEIVDIIKAVQRESRNAIE
                     AVQRSSKSVDDGVRVSHQAEDALKRISDSARRSTQMVREIARVAVEQTKSSKQVTDAI
                     NVVATTVQQVATATSEQARGAEQIMRSAEKMKTITRHVERSTQEQTRGSRQITRAIET
                     ISEMVSQLSKAHRAQAQGADTILDGLESVREGMQYQRSLIEQLAARVGLSPAPRRHAR
                     PGRNNK"
     gene            3806..4546
                     /locus_tag="VGB85_04685"
     CDS             3806..4546
                     /locus_tag="VGB85_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEY0133347.1"
                     /translation="MFGIGVTEMIVIGLVILMLFSPRELPGMMKTLARAYGSIRRTAE
                     EFRSQVMDAEELREPIEEIRAAYHGTKTELLSAQELARRELGRARAEAMKAQQKLSEL
                     AREESRREASVKSRTSEDAHAKAIGAAAGAAPAGTVAVGSGAPVGTVTASASSTSSPA
                     SAPAGTVAVPASAPAGTVAVGSGAPVHSSAPVGTVASSSPFASSAPTVATANTVPSPP
                     TTPTPAPPPPVTSPSFAPPRAPDRSDAA"
     gene            4543..5460
                     /gene="tatC"
                     /locus_tag="VGB85_04690"
     CDS             4543..5460
                     /gene="tatC"
                     /locus_tag="VGB85_04690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY0133348.1"
                     /translation="MTPVITNNGLDGLAEGPEGDTPMSFWDHLGELRSRLTKAALGMT
                     LGVVVCFIYATELREFLAGPLHKAWLAANMPGKPELQVLAMMDAFMTDMRVAMTGGIF
                     LAAPIGFYQFWMFIAPGLYAREKRFVVPFVATSVFMFLLGAAFCYYVVLPWTIQWLLE
                     YTRSTSAQTAVVYNLTLNEYIRDSTKVLVAFGAVFEFPLLIAFMAYAEMLTHKTLLRF
                     WKISVVAIFIIAAFLTPPEPVSQLMMAFPMVGLFFLSVGIAYLIGESKAKARALLAKE
                     TAEADAAEAARNPVAPDVDIDDDAPPPAV"
     gene            complement(5463..>6395)
                     /locus_tag="VGB85_04695"
     CDS             complement(5463..>6395)
                     /locus_tag="VGB85_04695"
                     /inference="COORDINATES: protein motif:HMM:NF033540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HEY0133349.1"
                     /translation="EKGSGCIGFRLYLPESWTEDRARCQKAGVPDDVVFETKWRLALQ
                     LVERALAWGVRKHVVLADAGYGEVTDFRDGLAAPGLTYVVGVPRGIVVWPPDTKFHVP
                     AHAPGKLGRPASRPIAEAASPTSIESLFAAQPRSAWKTVTWREGSRGKQSSRFCALRV
                     CTAHKHRSGHHPGEEQWVFAQWPRGEEHPTKYYLSNLPRGTALRTLVRLAKLRWRVER
                     DYQEMKGEVGLDHFEGRSWTGFHHHAALCAAAHAFLSLQRALFPPQDSELDPADGSPS
                     SSADPHRHDWDLPPLPAPCYTPLAAERALDHLIK"
BASE COUNT         1053 a         2318 c         2070 g          954 t
ORIGIN      
        1 tcgctgccgg acgtgctcgt attaccgccc tcgccgccac cctcgaccgg cagcccgccg
       61 ttgcagccat cgccgtcgta ctcgatcgcg ccgatgtcgt gcttgccgtc gtcacgcgcg
      121 ttgccgcaat agtcgtcggt cacctcgggg accgccaggc cgaggtcgac gagcatcgcg
      181 ccgtccttga gcgtgaagtc ataggcgccc gggtcctgga accacccgcc gagcacatcg
      241 aggccgacct cctcgaggtt gttcatcttc atcgacgtcc cgccgtcgcg gtcgcggacc
      301 ttgccgttca agatattgtt ggcgacgagg cccgtcgtct ccgcgaagcg catgtcgatg
      361 ccgctggtat tgtagagcgt attattgata atcttcgagt ccgccccggc gttgatgtac
      421 acgccgacat cggccgggca attgacgatg agattattac ggatgatccc ccgctcgtgc
      481 tccggcttgc acttcccgtc ctcgcagatc gagtcgggcc cggtgccgcc gccgccgagc
      541 gacagcccga ggcggacctg ccccgagtgc agcagctcgc agacgatcaa attgcgctcg
      601 atgatcccgt ccttggagtg ccccttcaaa aacgccgcgt agctgatatt gtcgccgccg
      661 cccttggcgt ggtcgtgaat gaagttcccg cggacgatcc agttgtgtcc gccgacgacg
      721 tcgaccggcg tcaccggatt tccagtgtcg cgcggcgcat cattatataa ctcgttgtat
      781 tcgatcgtga cgtcgtcggg ccacacatag ctcccgccca tcccgatcgg ctcgccgttg
      841 cccttgatca tcgcgttgaa gccgtgcatc ttgttgtgat gcaggtgggt gccgtcggcg
      901 gcgccggtga cgtggaacgc gtgctcgcag tcgctgtcgt tgccgcaggt cccctcgatg
      961 tcgaggttgg cgaactccca gttcgcgccg cgcacatgaa agccctcgag cgcgtcgaat
     1021 ttgatcaaaa ccgtgccgag ctcggccgcc tgcaccacga tcggcgcgtc ggccgtgccc
     1081 atcgcctcgc aggcgacctt gttctgctgg atctcgtagg tacccggcgc caggacgatc
     1141 gtgtctccag gctgggccgc ctggatcgcc gcgagcaggc ccggcacgtc cgccaccggg
     1201 acctcccccg cccgcgccgc cgagccgacc agcatggcgg atgccacaga aacaaaccca
     1261 agaccacgaa gcatgagacg atccatgccg gcaccctaac acccggcggt catcgctccg
     1321 ggcgcccaca aacccgcgcc ccgtgaagtg gcgcgtcgcc cggcgacccc acggggcggc
     1381 tttgaacatc gtttcccggg aactggccgg ctgatgcggt cgcgcctcgc ggacccctcc
     1441 tgtcctcacg gacaggtccg cgatcgcgcg tgcgcacccg gggagagccg gcgcatcggc
     1501 gtcgctctct ggagcttgtc tcgccgctcc cgtacaatcc ccccgctcgc acgatggacc
     1561 atcgcctccc tttgccgccc tgggtgccgc ttctgctcgg cctcgctgcc ctcgccgccg
     1621 cgttcgcgct gcaggccccg acggcggcca ccgccgcggc catcgcgggc atcgccgccg
     1681 gcgtcaccgg ctggctgatc gcctcgcggg ccgagctcgc cctcgggcgc gccgacgagg
     1741 accgcctcgc gctcaaggag gccgagggcc gctcgcgcca ggccgagcgc gccgtgcagg
     1801 accacggcgc gcaggtccag aacctgcaag cccagctgca aagcgcccag cagcaggtcc
     1861 aggccgccca gcagcagatc cgcggcgccg agctgcaggc ccaggagttc gccagccgcg
     1921 tccaggccct cgacggcgag aaccgtaacc tccagctcca ggtgcgcacc caggaggtcg
     1981 agacccggac gctgcaagct cgcctgcagg acctgaccac gcgggcgcac gacgccgagg
     2041 agcgcgtgcg cgtcctcgag tccgagctgc gtcaggcgca aaatcgcggc aactcgcgca
     2101 acgacgcccg ccaggacgcc gagctcgagg cgctgcggca cgaggtcgag gagcagcggc
     2161 ggaccgcgac cgagacgcgg gcgcgcctct cggccgagct cgacggcatg cgcgagacgc
     2221 tggcccgcaa cgaggtcgag ctgcgccagg cgcagacgcg cgtcgccgag gtgacgagcc
     2281 agcgcgacgg ggcgctgcgc gacgccaacc agctgcagac gatgatcgac cgcggcctgc
     2341 cctcgggcgg actcgacgac gaggcgaccg cgctgctgca cgagctcaac gagggcttca
     2401 cctcgtcggc gcaggcgatc gaggagacct cgcaggcgct gacgaccgtc aacggcgggc
     2461 tcaagggcat cgccgacaac gtcgagctgc tggcctccaa cgcggaggag agcgcgagct
     2521 cgatcctcga gatggcggcg gccaacgacg aggtcgccga gagcatgttg aacctcgcgg
     2581 cctccgtgca ggagaccgcg acctcgatcg aagagatgac cttctccgtc aaggaggtcg
     2641 ccaagaacat cgaggcgctg agctcgaccg ccgaggagac ctccgcggcg atgaacgaga
     2701 tggacggctc gatccagcag gtcgagaaca acgccaacga gaccgcgcaa ttgtcggagg
     2761 aggtcgtgtg ggcggccgag ggcggcgtcg acgcgatcaa ccagaccatc gaggtcatca
     2821 acctcatcaa gacctactcg gccgagggcg tgaccgtcat cggccggctc ggcgagcgca
     2881 tcagcgagat cggcaagatc ctgagcgtca tcgacgatgt ctccgagcag accaacctgc
     2941 tcgcgctcaa cgccgcgatc atcgcggccc aggccggtga gcacggcaag ggcttcgccg
     3001 tcgtcgccga cgagatcaag gacctcgccg agcgctccgc gacctctaca aaagagatcg
     3061 tcgacatcat caaggccgtg caacgcgaga gccgcaacgc gatcgaggcc gtgcagcgct
     3121 cgagcaagag cgtcgacgac ggcgtgcgcg tcagccatca ggccgaggac gcgctcaagc
     3181 ggatctccga ctccgcgcgc cggtcgaccc agatggtccg cgagatcgcc cgcgtcgccg
     3241 tcgagcagac caagagctcc aagcaggtca ccgacgcgat caacgtcgtc gccacgaccg
     3301 tgcagcaggt cgccaccgcg acctccgagc aggcccgcgg cgccgagcag atcatgcgct
     3361 cggccgaaaa aatgaagacg atcacgcgcc acgtcgagcg ctccacgcag gagcagaccc
     3421 gcggctcgcg ccagatcacc cgcgccatcg agaccatctc ggagatggtc agccagctca
     3481 gcaaggccca ccgcgcccag gcccagggcg ccgacacgat cctcgacggc ctcgagtcgg
     3541 tccgcgaggg catgcagtac cagcgcagcc tcatcgagca gctcgcggcc cgcgtcggcc
     3601 tcagcccggc cccgcgtcgc cacgcccgcc ccggccgcaa caacaagtaa tccgccgcgg
     3661 cccgcccgcc ccgcctcgcc tcacgcgggc gcgtgcgggt tctctggcgc cctcttataa
     3721 agggcgaagc cggcgggcgc agacgacgcg cccgggggcg cgcaggggct cgccgggggc
     3781 gcaggacgct gctactgtct gcacagtgtt cggcatcggc gtcacagaga tgatcgtcat
     3841 cgggctggtg atcctgatgc tgttctcgcc gcgcgagctg cccgggatga tgaagaccct
     3901 cgcccgcgcg tacggcagca tccgacgcac tgcggaggag tttcgttccc aggtcatgga
     3961 cgccgaggag ctgcgcgagc cgatcgagga gatccgcgcc gcctaccacg gcaccaagac
     4021 cgagctgctg tccgcccagg agctcgcccg ccgtgagctc ggccgggccc gtgccgaggc
     4081 gatgaaggcc caacaaaagc tgtccgagct cgcccgcgag gagagccgcc gcgaagcctc
     4141 cgtcaagagc cgcaccagcg aggacgccca cgcgaaggcc atcggcgcgg cagcaggcgc
     4201 cgctccggct ggcaccgtcg ccgtcggctc cggtgccccg gtcggcaccg taaccgcttc
     4261 cgcatcgtcc acatcatccc ccgcgagcgc cccggccggc accgtcgccg tgcccgcgag
     4321 cgccccggcc ggcaccgtcg ccgtcggctc tggcgccccg gtccactcga gcgccccggt
     4381 cggcaccgtc gcctcgagct ccccgttcgc cagcagcgcc ccgaccgtcg ccaccgcgaa
     4441 caccgtgccg agcccgccga caaccccgac gcccgctccg ccaccgccgg tcacgtcgcc
     4501 ctcgtttgcc ccgccgcggg cccctgaccg ctcggacgcc gcatgactcc agtgatcacg
     4561 aacaacggcc tcgacggcct cgccgagggc cccgagggcg acaccccgat gtcgttctgg
     4621 gatcacctcg gcgagctgcg cagtcgtctg accaaggccg cgctcggcat gacgctcggc
     4681 gtggtggtgt gtttcatcta cgccaccgag ctgcgcgagt tcctcgcggg gcccttacat
     4741 aaagcgtggc tcgccgccaa catgcccggc aagcccgagc tgcaggtgct ggcgatgatg
     4801 gacgccttca tgaccgacat gcgtgtcgcc atgaccggcg gcatcttcct cgccgcgccg
     4861 atcggcttct atcaattctg gatgttcatc gccccgggcc tgtacgcccg cgagaagcgc
     4921 ttcgtcgtcc ccttcgtggc gacgagcgtg ttcatgttcc tgctcggcgc ggcgttttgt
     4981 tactacgtcg tattgccgtg gacgatccag tggctgctgg aatatacgcg gtcgacgagc
     5041 gcccagaccg cggtcgtcta caacctcacc ctcaatgaat acatccgcga ctcgaccaag
     5101 gttctggtcg cgttcggggc cgtgttcgag ttcccgctgc tgatcgcctt catggcgtac
     5161 gcggagatgc tgacccacaa gacgctgctg cgcttctgga agatctccgt cgtggcgatc
     5221 ttcatcatcg ccgccttctt gaccccgccc gagccggtca gccagctgat gatggccttc
     5281 ccgatggtcg gcctgttctt cctcagcgtc ggcatcgcct acctgatcgg cgagtccaag
     5341 gccaaggcga gagccctgct ggccaaggag accgccgagg ccgacgccgc cgaggccgcc
     5401 cgcaacccgg tcgccccgga cgtcgacatc gacgacgacg ccccgcctcc tgccgtctag
     5461 tactacttga tcagatggtc gagggcccgc tcggcggcga gcggggtgta acacggcgcc
     5521 ggcagagggg gcaggtccca atcatgccga tgaggatctg ctgaagaact cggcgaacca
     5581 tcggcagggt ccagctccga gtcctgaggg gggaaaagcg ctcgctgaag actcaggaac
     5641 gcgtgggctg cggcgcagag ggctgcgtgg tgatggaagc cggtccaact gcgaccttcg
     5701 aagtggtcga ggccgacttc gcctttcatc tcttggtagt cgcgctcgac ccgccagcgt
     5761 agtttggcga gacggacgag ggtgcgtagc gccgtaccgc gcgggaggtt ggagaggtag
     5821 tatttcgtcg ggtgctcctc gccgcggggc cactgggcga agacccactg ctcttcgccc
     5881 gggtgatgac cgctgcggtg tttgtgggcc gtgcacacgc gaagggcaca gaagcgcgag
     5941 ctctgctttc cccgggaccc ttctcgccaa gtgaccgttt tccacgccga gcgcggctgc
     6001 gcggcaaata gcgattcgat actggtcgga ctcgccgcct cggcgatcgg ccgactcgcg
     6061 ggacggccga gcttgccggg cgcatgggcc ggaacgtgga acttcgtgtc cggcggccaa
     6121 acgacgatac ccctcgggac accgacgacg taggtaaggc cgggagctgc caggccgtcg
     6181 cggaaatcgg tgacttcgcc gtagccggcg tcggcgagga ccacgtgctt gcgtacgccc
     6241 cacgccagag ccctctcgac gagttgcagc gccagccgcc acttcgtctc gaagaccaca
     6301 tcgtcgggta cgcccgcctt ttgacaacga gcgcggtcct cggtccagct ctccggcagg
     6361 tacagacgaa agccgatgca gccgctgcct ttttc
//
LOCUS       DASWNJ010000258         1972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_76182, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000258 DASWNJ010000000
VERSION     DASWNJ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76182"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VGB85_04700"
     CDS             complement(<1..257)
                     /locus_tag="VGB85_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133350.1"
                     /translation="MTSRRLTTILATTLTAVTAPQLAGAAQPEGDAGGSAGVSAGASL
                     GTETEPDTSATASGSGSDGGSASGGGDTSGTTSGAPVVVSNV"
     gene            complement(382..711)
                     /locus_tag="VGB85_04705"
     CDS             complement(382..711)
                     /locus_tag="VGB85_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133351.1"
                     /translation="MSTQLPPATRMRLCLEDILRDRVPLMDGLDALLRLAEALPGLAE
                     DRDLRRIAEILAQAEHLPVGAARARWSAAVVARHDAELMEIERRNREAAHYCCRRLNE
                     TLAGVAG"
     gene            1299..>1972
                     /locus_tag="VGB85_04710"
     CDS             1299..>1972
                     /locus_tag="VGB85_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEY0133352.1"
                     /translation="MEPRMENVESEPSESAVEAPGFLAPYFRDLATVDVMTRDEELAA
                     AVRIATLRQTYWKSILSYPPFIDGICDLAREVLPAETCPGDALDAMKKASRALRDRDL
                     LVHQKAYEAAREALTLALAEADVDGLVSDRVLADLGSVEAGQNDGLSMKVKLPRRGSL
                     PFLNYVTSVRGNHQALWAAKNAFVKANLRLVVTIARRFNHGRLPLQDLIQEGNIGLMK
                     AVDRFD"
BASE COUNT          304 a          749 c          627 g          292 t
ORIGIN      
        1 acgttcgaca ccaccaccgg cgcgccgctc gtcgtgcccg aggtgtcgcc gccgcccgag
       61 gcgctgccgc cgtcgctgcc gctgccgctc gccgtggcgc tcgtgtcggg ctcggtctcg
      121 gtgccgagcg aggcgccggc cgagacgccg gccgacccgc ctgcatcccc ttcgggttgc
      181 gcggcgccag ccagctgcgg tgccgtcacc gcggtcaggg tcgttgcgag gatcgtcgtc
      241 aatctgcgtg aggtcatgtg cggggccctt tgtgtccgcg cagcttcacg cgcggcgctg
      301 cgatcgcaaa aaaacccgcg taacgttgtc tggcggccgc ccgcccgcgc cgcggagggc
      361 cagtgcggga gcgacgcccc tctagcccgc cacgcccgcg agcgtctcgt tcaagcgccg
      421 gcagcagtag tgcgccgcct cgcggttgcg ccgctcgatc tccatcagct ccgcgtcgtg
      481 gcgggcgacc accgcggcgc tccagcgggc ccgcgccgcc ccgaccggca ggtgctccgc
      541 ctgcgccagg atctcggcga tccgccgcag gtcacggtcc tcggccagcc ccggcagcgc
      601 ctctgccagc cgcagcaacg cgtcgagccc gtccatcaac ggcacccggt cgcgcaggat
      661 gtcctccagg cacaggcgca tgcgggtcgc cgggggcagt tgcgtgctca tcgcgcgagg
      721 ctatcccggt gtcgcaatca tctgacagtg gggtggatcc cagccgacga acaactggca
      781 aacccgccgc cgcggatcca ccaagtccca acaacgtgcc atgtgccaca agacggacaa
      841 ccggacatgc cacgcagacc actgcagagg tgcttttgcg gggtcgggag agatctcccg
      901 gacaccttgc atcgaccttc gacgcgcgcg ttacggcagt gtcagttgtg cgaggagtcc
      961 gccggctcgc gcgaagtgat cacagttttg ccagtcctcg ggtttccagg aaccggcggt
     1021 atttgagccc cgataccgcg aattgatggg attcgggccc tcctcggggc gcgtgatcag
     1081 attgaacgac acgttgacat cgcatcggga ctcccccaaa gtcgagacac ttcgaatcgc
     1141 ctgctgagcc cgcttattcg ggccccgggg cggtttggac agctccaaca agcttcgctc
     1201 cgaaggactc ctcgatgcaa aacgctacct cgaccgtatc cccgtccgtg gccaccaccg
     1261 cgcgccgcgg cgccgcagca gctgcccgta ccgggagcgt ggagccccga atggagaacg
     1321 tcgagagcga gcccagcgag agcgccgtcg aggcgccggg gttcctcgcc ccctacttcc
     1381 gcgacctcgc caccgtcgac gtgatgaccc gcgacgagga gctcgccgcc gccgtccgca
     1441 tcgcgaccct gcgccagacc tactggaagt cgatcctctc ctatcccccc ttcatcgacg
     1501 gcatctgcga cctcgcccgc gaggtcctgc cggccgagac ctgcccgggc gacgccctcg
     1561 acgccatgaa gaaggcctcg cgcgccctgc gtgaccgcga cctgctcgtg catcagaagg
     1621 cctacgaagc cgcccgcgag gcgctgacgc tcgcgctcgc cgaggccgac gtcgacggcc
     1681 tcgtctccga ccgcgtgctc gccgacctcg gcagcgtcga ggccggccag aacgacggcc
     1741 tgagcatgaa ggtcaagctc ccgcgccgcg gcagcctgcc cttcctcaac tacgtcacca
     1801 gcgtgcgcgg caaccatcag gcgctttggg ccgccaagaa cgcgttcgtc aaggccaacc
     1861 tgcgcctcgt cgtcacgatc gcccgccgct tcaaccacgg ccgcctgccg ctgcaggacc
     1921 tgatccagga gggcaacatc ggcctgatga aggcggtcga ccgcttcgac ca
//
LOCUS       DASWNJ010000259         2623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_181311, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000259 DASWNJ010000000
VERSION     DASWNJ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181311"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VGB85_04715"
     CDS             <1..576
                     /locus_tag="VGB85_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133353.1"
                     /translation="WIFIFVWIGTYFLTHYFFDILGMRYAFPAGWTLDAALVGSGRGE
                     VPAFMYPLTQPYFVSYHVAMIVVLRRVHRALGLPHLSPGTARWPRLLALASVAVALSY
                     TVAFAETLFMASPVISDLFWYADKPRMLRWGSLFYACYFFISLPLLYRMDEPPGPKYS
                     PGRASLEALAAGMLVMMILDALALLLGPLVP"
     gene            complement(587..1093)
                     /locus_tag="VGB85_04720"
     CDS             complement(587..1093)
                     /locus_tag="VGB85_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133354.1"
                     /translation="MSSEVYDGDFGGLDGADLRCRNLAQAAGITSWPSFRAWLSDAEV
                     GPLERFTLVPAKPYGLPTGERVADSLADLVLNGPGDGIRVDELGKPLTPSLVWTNTGV
                     AGEPHSATNHCKHWDSASADVLARVGYSHVAKLPEDKWKAWRDQRWWTSYLLQTCDWM
                     ARLYCFEN"
     gene            complement(1706..>2623)
                     /locus_tag="VGB85_04725"
     CDS             complement(1706..>2623)
                     /locus_tag="VGB85_04725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0133355.1"
                     /translation="ARRRFLMLQVSVHACMLPGLVSLSLLAACGLERDWAAFQEAFAG
                     DSASSGTGSTTRAPEDSEGPGGDTTEEASTGSSGGSSGGESTGGGAAAGTTSEDTSTG
                     ATSEPSEVCGDGVVAGEEECDDGNDEPTDLCDNECARAWTIFVTSVGYDGNINGLVGT
                     GNRCRNLAGMANLPRWEKYVALISDSTTNAADRLHHAKGYYRLVNGLPVAHGWDALMT
                     GPLEYPINVTELSTTLSTAVWTGSLPGGTAVPGSEHCEGWMTDSPFVYGHIGSSSSVD
                     SGWIYLTNPDINPTPCAVASKSLYCVEQP"
BASE COUNT          380 a         1020 c          766 g          457 t
ORIGIN      
        1 tggatcttca tcttcgtctg gatcgggacc tacttcctga cccactactt cttcgacatc
       61 ctcggcatgc gctacgcctt ccctgccggc tggaccctcg acgccgcgct cgtcggcagc
      121 ggccgcggcg aggtcccggc cttcatgtac ccgctgaccc agccctactt cgtcagctac
      181 cacgtcgcca tgatcgtcgt gctgcgccgg gttcatcgcg ccctcggcct cccgcacctg
      241 tccccgggga cagcccggtg gccgcgcctg ctcgccctcg ccagcgtcgc cgtcgccctc
      301 tcctacaccg tcgccttcgc cgagaccctg ttcatggcca gtccggtgat ctccgacctg
      361 ttctggtacg ccgacaagcc gcggatgctg cgctggggct cgctattcta cgcctgctac
      421 tttttcataa gcctgcccct gctctaccgc atggacgagc ccccgggccc gaaatacagc
      481 cccggccgcg cctccctcga ggccctcgcc gccggcatgc tcgtcatgat gatcctcgac
      541 gccctcgccc tgctgctcgg cccgctcgtg ccctgaaggc gcgcggtcaa ttctcgaagc
      601 aatacagtcg ggccatccag tcacaggttt gcagcaagta actggtccac cagcgctggt
      661 ctcgccacgc cttccactta tcttccggca acttggccac gtgactgtac ccgacccggg
      721 ccaggacatc ggcgctggcg ctgtcccagt gcttgcagtg gttggtcgcg ctgtgcggct
      781 cgccggcgac ccccgtgttg gtccagacga gcgacggcgt cagcggcttg ccgagctcgt
      841 cgacgcggat gccgtcgccc gggccgttga gcaccagatc cgcaaggctg tcggcgacgc
      901 gctcaccggt cggcagtcca tacggtttgg ccgggacgag cgtgaagcgc tccagcggcc
      961 cgacctcggc gtcggagagc cacgcacgaa agctcggcca cgaggtgatc ccggccgcct
     1021 gggcgaggtt gcgacagcgc aggtcggccc cgtcgagccc gccgaagtca ccgtcgtaca
     1081 cctcgctgct caagaacacc aacctcgcgt cccatctgca ccccaccgtg cagggaacca
     1141 ggttttcttg gctctggccg ctaccatttt gcgcccccgc atcgcactgc tcctcgaggt
     1201 cctgcacacc atcgccgcaa aacgcgttcc actggcaatc cccgcagcca ccgtacgcat
     1261 cactattatc cggcccaaag tcgcaggcct cgacccccgc atggaccctg ccgtcgccac
     1321 agaacgccgt tttgcagtcg gttgaacagg cgccgccgtc actgttgccc accccctcgt
     1381 cgcactgctc tcccggatcg acctgaccgt caccacacat cgcaggcaag cccgtactgc
     1441 cctcgccgct cgtgcccccg gacgtgtcgt cgaccggacc gccggtcgtc cccgtgctgc
     1501 ccggcccggg ttccccggag gtcgtcggcg tccccggttc cggcacagcc gtcgagcccg
     1561 tcgagcccgt cgaatccgcg agatccatcg cgctgtcgga ggcaccctca ccgggcgtgc
     1621 acgcgagccc agcgacgtac aggacagcca cgaggcaaaa aaatgaaaca ggccagcgaa
     1681 tcaacatgat gcgttcctct gaaagtcagg gttgttcgac acagtagagc gacttgctcg
     1741 ccacggcgca cggcgtgggg ttgatgtctg gattggtcag gtagatccac ccggagtcga
     1801 ccgagctgct gctgccgatg tgcccgtaga cgaaaggaga gtcggtcatc cagccctcgc
     1861 agtgctcgga gcccggcacc gccgtgccgc ccggcaacga cccggtccag acggccgtcg
     1921 acagcgtcgt gctcagctcc gtcacgttga tcgggtactc cagcggcccg gtcatcagcg
     1981 cgtcccagcc gtgggccacc ggcaggccgt tgaccagccg gtagtacccc ttcgcgtggt
     2041 gcaggcgatc ggccgcgttc gtggtgctgt cgctgatcag cgccacgtac ttctcccacc
     2101 gcggcaggtt cgccatcccg gcgaggttgc gacagcggtt gcccgtgccg accagcccgt
     2161 tgatgttccc gtcgtagccg acgctcgtca cgaagatggt ccacgccctc gcgcactcgt
     2221 tgtcacacag gtcggtcggc tcgtcgttgc cgtcgtcgca ctcctcctcc cccgccacca
     2281 cgccgtcgcc gcagacctcg gacggctcgc tggtcgcccc ggtgctcgtg tcctcactcg
     2341 tcgtcccggc cgccgcgccc ccgcccgtgc tttcgccgcc cgagctaccg cccgagctcc
     2401 cggtcgaggc ctcctccgtc gtgtcgccgc cggggccctc gctgtcctcc ggcgcccgtg
     2461 tcgtcgaccc cgtgccgctg ctcgcggagt cgccggcgaa cgcctcctgg aacgccgccc
     2521 agtcgcgctc gagcccacaa gccgccagca gggacaggct cacgaggccg ggaagcatgc
     2581 aggcatgtac cgatacttgc agcatcaaaa atctcctccg agc
//
LOCUS       DASWNJ010000260         2743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_252764, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000260 DASWNJ010000000
VERSION     DASWNJ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252764"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /gene="dhaK"
                     /locus_tag="VGB85_04730"
     CDS             <1..958
                     /gene="dhaK"
                     /locus_tag="VGB85_04730"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: protein motif:HMM:TIGR02363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEY0133356.1"
                     /translation="LNEALRGFGAAHSDLVRVNTEPTFVTRATLTSAGKVALISGGGS
                     GHEPMHAGLVGVGMLDAACPGQVFTSPTPDQMLAAAQAVDSGAGVLFVVKNYEGDVMN
                     FEMAAEMYEGGSHATVLTNDDVAVEDSLFTTGRRGVAGTVVVEKIVGAAAEAGADLEA
                     LKALGDKVNGRTRSMGVALTSCTVPAAGRPTIEIGEGEMEMGVGIHGEPGRRRVPLKP
                     ADEIAAEMVEAIARELAAPAGTGALLLVNGFGGTPAMELYLMYDAARRALEGKGITIA
                     RSLVGSYCTSLEMAGCSLTVTALDDELAGQWDAPVHTAALRW"
     gene            complement(993..2198)
                     /locus_tag="VGB85_04735"
     CDS             complement(993..2198)
                     /locus_tag="VGB85_04735"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEY0133357.1"
                     /translation="MRIRITDLRCAVIGDSPIVRITTDAGIDGYGEVERYKPYLKPFV
                     LHLREGLIGEDPTDVERVMLKIRSRGAFKPYGSAVSVIEMALWDIAGKAAGVPVYKLL
                     GGKVRDRVRVYNGAHRTPMTGQEPEDYAEDVRRMRALPQGFTIVKQGIAFHSPMKRRP
                     DFSYGEPPRPTPFHGYVDRGLLTERGMKYLIACVEAMKEVLGDEVGLALDCGPGFTVP
                     DAIRFARAVEPLNLMWLEDMLTGDYVPYVDADVYREVTRATTTPIHTGEQIYLRQNFK
                     GLIETRAVSIVGPDPCDVGGIAELKRVAEYADLHGVLIAPHGTGNGVLGTAALVQVCA
                     TLPHNFIAFECPRGDPDWWPGLVDGLPDPLIQGGMIEVWDAPGMGAMLNPERTKPHLR
                     EEDAAFFDD"
     gene            complement(2195..>2743)
                     /locus_tag="VGB85_04740"
     CDS             complement(2195..>2743)
                     /locus_tag="VGB85_04740"
                     /inference="COORDINATES: protein motif:HMM:NF006093.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133358.1"
                     /translation="LYGDDEYADPERQLHRHVLYHTKPGDIVVVDARADMRSGVFGEM
                     MLTYFQGRGGAGVIIDGCLRDWPHVQTLDLGLWLRGVTPNFHTQTDIMPFGVNVPIAC
                     GGVLVMPGDIIVADDDGAVAVPVALAQQVIEQAHVRHEWEDFSRERLAAGGDLRVYYP
                     LSDAARPEYEAWLAKQSKGPAR"
BASE COUNT          380 a          955 c          997 g          411 t
ORIGIN      
        1 cctgaacgag gcgcttaggg gcttcggcgc ggcgcattcc gacctcgtca gggtcaacac
       61 cgagcccacc ttcgtcaccc gcgccacgct gacttctgcc ggcaaggtgg cgctgatctc
      121 cgggggcggc tcgggtcacg agccgatgca cgcgggcttg gtaggcgtcg gaatgctgga
      181 cgccgcctgc ccgggccaag tgttcacctc gcccacgccc gaccagatgc tggcggcagc
      241 ccaggcggtc gactccggtg ccggcgtgct gttcgtcgtc aagaactacg agggcgacgt
      301 gatgaacttc gagatggcgg ccgagatgta cgagggcggc agccacgcca cggtgctcac
      361 caacgacgac gtcgccgtcg aggactcctt gttcaccacc ggccgccgcg gcgtggccgg
      421 cacggtggtc gtcgagaaga tcgtgggcgc agcggcggag gcgggcgccg acctggaggc
      481 gctcaaggcg ctgggcgaca aggtcaacgg ccgcacccgc tcgatgggcg tggcgctcac
      541 ctcctgcacc gtgcccgccg ccggcaggcc caccatcgag atcggcgagg gcgagatgga
      601 gatgggggtg ggcatccacg gcgagcccgg ccgccgccgc gtcccgctca agcccgccga
      661 cgagatcgcg gccgagatgg tcgaggccat cgcccgcgag ctggccgcac cggcgggcac
      721 cggcgccctc ctgctcgtca acggcttcgg cggcacgccc gcgatggaac tctacctgat
      781 gtacgacgcg gcgcggcgcg cgctggaagg taagggcatc accatcgccc gctcgctcgt
      841 cggcagctat tgcacctcgc tcgagatggc cgggtgctcg ctcaccgtga ccgcgctgga
      901 cgacgagctc gccggccagt gggacgcccc ggtccacacc gcggcgctgc gctggtgacg
      961 tctcaggcgc cgcgcgctca ggcgccgcgc gctcagtcgt cgaagaacgc ggcgtcctcc
     1021 tcgcggaggt ggggcttggt tctctcggga ttgagcatgg cgcccatgcc cggggcgtcc
     1081 cagacctcga tcatgccgcc ctggatgagc gggtcgggca ggccgtcgac gaggcccggc
     1141 caccagtccg ggtcgccgcg cgggcactcg aaggcgatga agttgtgggg cagggtggcg
     1201 cagacctgga ccagcgccgc cgtgcccagc acgccgttgc cggtgccgtg gggggcgatg
     1261 agcaccccgt gcaggtcggc gtactcggcc acgcgcttga gctccgcgat gccgcccacg
     1321 tcgcacgggt cgggtccgac gatgctcacc gcgcgggtct cgatcaggcc cttgaagttc
     1381 tggcgcaggt agatctgctc gccggtatgg atcggggtcg tcgtcgcccg cgtgacctcg
     1441 cggtagacgt ccgcgtcgac gtaggggacg tagtcgccgg tgagcatgtc ctcgagccac
     1501 atcaggttca gcggctcgac cgcgcgcgcg aagcggatcg cgtcgggcac cgtgaagccc
     1561 ggcccgcagt cgagggcgag gcccacctcg tcgcccagca cctccttcat cgcctcgacg
     1621 caggcgatga ggtacttcat gccgcgctcg gtgaggaggc cgcggtcgac gtagccgtgg
     1681 aacggggtgg gccgcggcgg ctcgccgtag gagaagtccg ggcgacgctt catcgggctg
     1741 tggaacgcga tgccctgctt cacgatggtg aagccttgtg gcagcgcccg catccgccgg
     1801 acgtcctccg cgtagtcctc cggctcctgg ccggtcatcg gcgtgcgatg ggcgccgttg
     1861 tagacccgga cccggtcgcg caccttgccg ccgagcagct tgtagaccgg tacgcccgca
     1921 gccttcccgg cgatgtccca cagcgccatc tcgatgacgc tgacggcgct gccgtacggc
     1981 ttgaaggcgc cgcgcgagcg gatcttgagc atgacccgct cgacgtcggt cgggtcctcg
     2041 ccgatcagcc cctcgcggag gtgcaggacg aagggcttga ggtagggctt gtagcgctcg
     2101 acctcgccgt agccgtcgat gcccgcgtcg gtcgtgattc gcacgatcgg gctgtcgccg
     2161 atgacggcgc agcggagatc ggtgatcctg atcctcatcg ggccggtccc ttcgactgct
     2221 tggccagcca cgcctcgtac tcggggcggg cggcgtcgct gagcgggtag tagacgcgca
     2281 ggtcgccgcc ggcggccagc cgctcgcgcg agaagtcctc ccactcgtgc cggacgtggg
     2341 cctgctcgat cacctgctgc gccagcgcca ccggcacggc caccgccccg tcgtcgtcgg
     2401 cgacgatgat gtcgcccggc atgaccagca ccccgccgca ggcgatcggg acgttgacgc
     2461 cgaacggcat gatgtcggtc tgggtgtgga agttgggcgt cacgccgcgc agccacaggc
     2521 cgaggtcgag cgtctggacg tgcggccagt cgcgcaggca gccgtcgatg atcacgccgg
     2581 cgccgccgcg gccctggaag taggtgagca tcatctcgcc gaacacgccg ctgcgcatgt
     2641 cggcccgcgc gtcgaccacg acgatgtcgc cgggcttggt gtggtagagc acgtgccggt
     2701 gcagctggcg ctcggggtcg gcgtactcgt cgtcgccgta gag
//
LOCUS       DASWNJ010000261         5694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_110944, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000261 DASWNJ010000000
VERSION     DASWNJ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110944"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VGB85_04745"
     CDS             <1..409
                     /locus_tag="VGB85_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133359.1"
                     /translation="GTIIGSFGSRTVAAVDREGRVSLSLLETSRGNVPRKISPKASKL
                     PLAPATGPLAPGLGRGFPFFLVPYGDAAPVHVIASDGLDYFVAPLADLHCDALALAVT
                     LAGNDPEQAALPLACLADGELRLGAVHIDPPAP"
     gene            465..1562
                     /locus_tag="VGB85_04750"
     CDS             465..1562
                     /locus_tag="VGB85_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133360.1"
                     /translation="MSLTKRILIGLTILLLVVVGAAAWMFRSRAEAIPDQLAPIDTAE
                     ADPSKIVIPGRGGLPDLVLADLRGKTVYLLVGDRESSQARESMLFDRALGRWDIPQDV
                     VGFSIADTEGLRYLASVIEGFIDPMRPELRLPLHIDYDGAITRAFKLPKGHAGVVVLG
                     PGGEILMRHSGPPPAGPEGDKVIEQLRTHLKAKEPTLAPAPAFKLGTLDNTACKGKVC
                     IFVFLARPVKKTDLPGVEGGFDGDTDAMWKQLGDPSVRLASLVHDSDTKLVAADEKAA
                     KADKPATPPAPRVEAVLVGALADVELKRWTTVPAAPEARAALQIPEDQAGVVIIDADG
                     NLAVREVGVVRMYKFTRISELLGVDLSDRRE"
     gene            complement(1564..2274)
                     /locus_tag="VGB85_04755"
     CDS             complement(1564..2274)
                     /locus_tag="VGB85_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0133361.1"
                     /translation="MAAPPPSLKDEKRALWYLRKIPLLEGLSTDQYRELIGLVEVREL
                     QRRQIVYLPGDPGEHVFFIQGGRIKCSKVSRDGKELTLTYVAAGEFFGELCVLEGQPR
                     EEMAEAMKNSIITLLPREKICALLEAESHIAFRFIKVIGDRRRSMETKIEQLVFRDVH
                     AKLAALLLELGREYGKDTEDGVQIDVKITHQEMANLIGSTRETISLTLAAFKRKELIQ
                     LNGRTVVLTDQEGLTALT"
     gene            2499..4043
                     /locus_tag="VGB85_04760"
     CDS             2499..4043
                     /locus_tag="VGB85_04760"
                     /inference="COORDINATES: protein motif:HMM:NF017568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IG family 5'-nucleotidase"
                     /protein_id="HEY0133362.1"
                     /translation="MSLASGLPTPIRKLLAEAELVLEAPFARRIFTNRDLAFDQIPVI
                     GFDMDYTLALYRQDELEALSIRCTLDKLLTRGYPIALRDIKSDPAFAIRGLVVDKKFG
                     NIIKMDRHGYVGRAYHGKHMLPRPERKSLYRSQRLGTEKERFAAVDTLFALPEVTLFA
                     EVVDLIDHHPELWTSGGPPSYAQAWSDVRECIDKAHQDGSLKDLIQADIGRYFQIDPD
                     LGSTLHKFRSAGKKLFLLTNSLLPYTDHVMRYVLGGQMPAYDHWRDYFDWIVVGAAKP
                     NFFTGNAPFQELDISDKPIGKPVAEPKRGRIYLGGNQQGLQTALGVHADEVLYVGDHI
                     YGDIVKSKKSSGWRTALIVEDLEHELDVRRDRQVTLKEIEHLTWLRISLSEDISSQRY
                     LSRLLARMTPEDLVRQGTPASEAEQMLTDTRATVRKRFDRLRRYEEEVADTLERRIID
                     VDEAFNPYWGSVFAARRDTSRFGAQVENYACLYTSRVTNFRYASPTKYFHSPHGSMPH
                     WHGPPK"
     gene            4046..4465
                     /locus_tag="VGB85_04765"
     CDS             4046..4465
                     /locus_tag="VGB85_04765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEY0133363.1"
                     /translation="MKSHREELWFETDTRRAYLNITERVAEAVRTSGVREGLCLVNAM
                     HITASVYINDDEPGLIADYDDFLERLAPHEPTSQYRHNRTGEDNGDAHIKRQLMGREV
                     VVAITDGALDLGPWEQIFYAEFDGRRRKRVLVKIIGE"
     gene            4724..>5694
                     /locus_tag="VGB85_04770"
     CDS             4724..>5694
                     /locus_tag="VGB85_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0133364.1"
                     /translation="MADQNPLLSDRDVQFQLHELNDVRALCRLPAYAEHSAETFELFL
                     RSARSLARNVLFPAYRPMDAEPPRFEGGRITVHPAMRAIWGQMAELGILSATRPVEVG
                     GQQLPLMVAIVAQAYLMAANLGAYGFAGLTTGAAHLLEAFGDEYLRENFMRRMYAGEW
                     TGTMALTEPQAGSSLGDITTRATLHADGRYRVAGHKIFISGGDHDLTDNVVHLVLARL
                     DGAPPGVKGISLLAVPRRRPTADGGLEDNDVSTAGIIHKIGWKGLPSLVINFGEQADC
                     HGWLVGQPHQGLAYMFQMMNEARIMVGVNGMATASAAYHEAVRYARDR"
BASE COUNT          936 a         2164 c         1747 g          847 t
ORIGIN      
        1 cggcacgatc atcggcagct tcggcagccg caccgtcgcc gccgtcgacc gcgagggtcg
       61 cgtcagcctc agcctgctcg agaccagccg cggcaacgtg ccacgcaaga tctccccgaa
      121 ggccagcaag ctgccgctcg cgcccgcgac cggcccgctc gcgcccggcc tcggccgcgg
      181 cttcccgttc ttcctcgtcc cgtacggcga cgccgccccc gtgcacgtga tcgccagcga
      241 cggcctcgac tacttcgtcg cgccgctcgc cgacctccac tgcgacgccc tcgccctcgc
      301 cgtcaccctc gccggcaacg accccgaaca ggccgcgctc ccgctcgcct gcctcgccga
      361 cggcgagctg cgcctcggcg ccgtgcacat cgacccgccg gccccctgaa aaccgcgcgt
      421 gggccccgct ggcgaggccc gggcgcccgt gttactccgg gcccgtgtcg ctcaccaagc
      481 gcattctcat cggcctgacg atcttgctgc tcgtcgtcgt cggcgcggcg gcctggatgt
      541 tccgctcgcg ggccgaggcg atccccgacc agctcgcgcc gatcgacacc gccgaggccg
      601 acccgagcaa gatcgtgatc cccggccggg gcggcctgcc ggacctcgtg ctcgccgacc
      661 tgcgcggcaa gaccgtctac ctgctcgtcg gcgaccgcga gagctcgcag gcccgcgaga
      721 gcatgctgtt cgaccgcgcg ctcggtcgct gggacatccc gcaggacgtc gtcggcttca
      781 gcatcgccga caccgagggc ctgcgttacc tcgccagcgt gatcgagggc ttcatcgacc
      841 ccatgcgccc cgagctgcgc ctgccgctcc acatcgacta cgacggtgcg atcacccgcg
      901 ccttcaagct gcccaagggc cacgccggcg tcgtcgtgct cggccccggc ggcgagatcc
      961 tcatgcgcca cagcggcccg ccgcccgcgg gcccggaggg cgacaaggtc atcgagcagt
     1021 tgcgcaccca cctcaaggcc aaggagccga ccctcgcgcc cgcgcccgcc ttcaagctcg
     1081 gtacgctcga caacaccgcc tgcaagggca aggtctgcat cttcgtgttc ctcgcgcggc
     1141 cggtcaaaaa gaccgacctg cccggcgtcg agggcggctt cgacggcgac accgacgcga
     1201 tgtggaagca gctcggcgac ccgagcgtgc gcctcgccag cctcgtccac gacagcgaca
     1261 ccaagctggt cgccgccgac gaaaaggccg cgaaggccga caagcccgcg actccccccg
     1321 cacccagggt cgaggccgtg ctcgtcggcg cgctggcgga cgtcgagctc aagcgctgga
     1381 ccaccgtacc cgccgctccc gaggcccgcg ccgccctgca gatccccgag gatcaggccg
     1441 gcgtcgtcat catcgacgcc gacggcaacc tcgccgtccg cgaggtcggc gtcgtccgca
     1501 tgtacaagtt cacccgcatc tccgagctgc tcggcgtcga cctcagcgac cgccgcgagt
     1561 aattcacgtc agggccgtca ggccctcctg gtccgtcagc accaccgtgc ggccgttgag
     1621 ctggatcagc tccttgcgct tgaacgccgc gagcgtcagc gagatcgtct cgcgcgtcga
     1681 gccgatcagg ttggccatct cctggtgtgt gatcttcacg tcgatctgca cgccgtcctc
     1741 cgtgtccttg ccgtactcgc ggccgagctc gagcagcagc gccgcgagct tggcgtgcac
     1801 gtcgcggaac accagctgct cgatcttcgt ctccatcgag cgcctgcggt cgccgatcac
     1861 cttgatgaag cgaaacgcga tgtgcgactc ggcctcgagc agcgcgcaga tcttctcgcg
     1921 cggcagcagc gtgatgatcg agttcttcat cgcctcggcc atctcctcgc gcggctggcc
     1981 ctcgagcacg cacagctcgc cgaagaactc gcccgccgcg acgtaggtca gcgtcagctc
     2041 cttgccgtcg cgcgagacct tgctgcactt gatccggccg ccctggatga agaacacgtg
     2101 ctcgccgggg tcgcccggca ggtacacgat ctggcgccgc tggagctctc tcacctcgac
     2161 cagcccgatc agctcgcggt actggtcggt cgataacccc tccagcagtg ggatctttcg
     2221 caggtaccag agcgcgcgct tttcatcctt gagcgagggg ggtggtgcgg ccatgcgggt
     2281 tccttcccca cggtacggaa gcccacgaat tcccgcaaca gcgtgatgcg gacgacgaac
     2341 ggggccactt tggtaagcct gaacaccaac gccgaccgcc gtgaaagccc tccgctttca
     2401 ccacaaacct accaaatcac aactactgtc ccggtacatc ctgcgatcca gaacgcaaag
     2461 gggtccgaag accacccctg ctaggctgct ggcttcgcat gtccctcgcg tctggcctcc
     2521 cgacaccgat ccgcaagttg ctcgccgaag ccgagctcgt gctcgaggca ccgtttgctc
     2581 gccggatctt caccaaccgt gacctcgcct tcgaccagat cccggtcatc ggcttcgaca
     2641 tggactacac gctcgcgctc taccgacagg acgagctcga agcgctgtcg atccgctgca
     2701 ccctcgacaa gctgctgacg cgcgggtacc ccattgcgct gcgcgacatc aagtccgacc
     2761 cggcgttcgc gatccgcggc ctcgtcgtcg acaagaagtt cggcaacatc atcaagatgg
     2821 accgccacgg ctacgtcggc cgcgcctacc acggcaagca catgttgccc cggcccgagc
     2881 gcaagtcgct gtaccgctcg cagcgcctcg gcaccgagaa ggagcgcttc gccgccgtcg
     2941 acacgctgtt cgccctgccc gaggtgactc tcttcgccga ggtcgtcgac ctgatcgatc
     3001 accacccgga gctgtggacc agcggtggtc cgccgagcta cgcgcaggcg tggagcgacg
     3061 tgcgcgagtg catcgacaag gcccaccagg acggctcgct caaggacctg atccaggccg
     3121 acatcgggcg ctacttccag atcgaccccg acctcggctc gacgctgcac aagttccgca
     3181 gcgccggcaa gaagctgttc ctgctgacga acagcctcct gccgtacacc gaccacgtga
     3241 tgcgttacgt gctcggcggc cagatgccgg cctacgatca ctggcgcgac tacttcgact
     3301 ggatcgtcgt cggcgcggcc aagccgaact tcttcaccgg caacgccccg ttccaggagc
     3361 tcgacatctc cgacaagccg atcggcaagc cggtcgccga gcccaagcgc ggccggatct
     3421 acctcggggg caaccagcag ggcctgcaga ccgcgctcgg cgtgcacgcc gacgaggtgc
     3481 tctacgtcgg ggaccacatc tacggcgaca tcgtcaagag caagaagtcg tccggctggc
     3541 gcaccgccct gatcgtcgag gacctcgagc acgagctcga cgtccgccgc gaccgccagg
     3601 tcaccctcaa ggagatcgag cacctgacct ggctgcgcat ctcgctgtcc gaggacatca
     3661 gctcgcagcg ctacctctcg cgcctgctcg cgcgcatgac ccccgaggac ctcgtccgcc
     3721 agggcacgcc cgccagcgag gccgagcaga tgctgaccga cacgcgcgcc accgtgcgca
     3781 agcgcttcga tcgcctgcgc cgctacgagg aggaggtcgc cgacaccctc gagcgccgca
     3841 tcatcgacgt cgacgaggcc ttcaacccct actggggctc ggtgttcgcc gcccgccgcg
     3901 acaccagccg cttcggcgcg caggtggaga actatgcgtg cctgtacacc agccgcgtca
     3961 ccaacttccg ctacgcctcg ccgaccaaat acttccactc gccgcatggt tcgatgcccc
     4021 actggcacgg cccgccgaaa tgaccatgaa gtctcaccgc gaggagctat ggttcgagac
     4081 cgacacgcgt cgggcctacc tcaacatcac cgagcgggtc gccgaggccg tgcgcaccag
     4141 cggcgtgcgc gagggcctgt gcctggtcaa cgcgatgcac atcaccgcca gcgtctacat
     4201 caacgacgac gagccgggcc tgatcgcgga ctacgacgac ttcctcgagc gcctcgcccc
     4261 gcacgagccg acctcgcagt accgccacaa ccgcaccggc gaggacaacg gcgacgccca
     4321 catcaagcgc cagctgatgg gccgcgaggt cgtcgtagcg atcaccgacg gcgccctcga
     4381 cctcggcccg tgggagcaga tcttctacgc cgagttcgac ggccgccggc gcaagcgcgt
     4441 gctcgtgaag atcatcggcg agtgagcccc cggctcctcg cggacaccga atagagacac
     4501 cgccgacgac gcccacacca cgtgggcgac gccggcggat cggttcgcag cacccctgac
     4561 ctctgcacag acactccccg gaacaggggc ccggaggccc gtgtcgctcg gttcgttcta
     4621 atggaatcat gtcccgtata atggcggccg tcaagcggcc tccgagaaaa atttccggga
     4681 cctgcgtaca cgggcgcctg gcgctgcatt tatgctgcgc gcgatggccg accagaaccc
     4741 gcttttgtcc gaccgtgacg tgcagtttca gctccacgag ctcaacgacg tgcgggcgct
     4801 gtgtcgcctg ccggcctatg ccgagcacag cgccgagacc ttcgagctgt tcttgcgcag
     4861 cgcccgcagc ctcgccagga atgtgttgtt tccggcgtac cggccgatgg acgccgagcc
     4921 gccgcgcttc gagggcgggc ggatcaccgt gcatccggcg atgcgcgcga tctggggtca
     4981 gatggccgag ctggggatcc tcagcgccac gcggccggtc gaggtcggcg gtcaacagct
     5041 gcccttgatg gtcgcgatcg tcgcgcaggc ctacctgatg gccgcaaacc tcggcgccta
     5101 cggcttcgcc ggcctgacca ccggcgccgc gcacttgctc gaggccttcg gcgacgagta
     5161 cctgcgcgag aacttcatgc gccgcatgta cgccggcgag tggaccggca cgatggcgct
     5221 gaccgagccg caggccggct cgagcctcgg cgacatcacc acgcgcgcga cgctgcacgc
     5281 cgacggccgc taccgcgtcg ccggccacaa gatcttcatc tccggcggtg atcacgacct
     5341 gaccgacaac gtcgtgcacc tggtgctcgc gcgcctcgac ggcgccccgc cgggggtcaa
     5401 gggcatctcg ctgctcgccg tgccgcgccg ccgcccgacc gcggacgggg gcctcgagga
     5461 caacgacgtc agcaccgccg gcataatcca caagatcggc tggaagggcc tgccgagcct
     5521 cgtgatcaac ttcggcgagc aggccgactg ccacggctgg ctggtcggcc agccgcacca
     5581 gggcctcgcc tacatgttcc agatgatgaa cgaggcgagg atcatggtcg gcgtcaacgg
     5641 catggcgacc gcctcggccg cgtaccacga ggcggtccgt tacgcccgcg accg
//
LOCUS       DASWNJ010000262         3489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_357474, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000262 DASWNJ010000000
VERSION     DASWNJ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357474"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..2162)
                     /locus_tag="VGB85_04775"
     CDS             complement(99..2162)
                     /locus_tag="VGB85_04775"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0133365.1"
                     /translation="MPRRSVHSPRSRPDVHAPTMRAVSVLSTISPVLLALACHREVAN
                     TAPPRPPAVAPAPVRPPRLELDAARMSADIATLAADDFNGRYTLSPDLRRAAELLAKR
                     HAELGLSPVGADFMIDFPLRTGARLREAASLELFKPGAAAKPTTLAAPEFAVVAQSGS
                     GAVRGELVFAGYAAQSEPPKPPGAGEPGAGKQSELVAAPYDDLAGIDLKGKVALVLLE
                     VPGRPDPLTLFMRLQEESQRFTDAAAPLKAAGDLRGLTKLHTSAQKRLYALMRPFMPQ
                     LAADELWPLPQDVATVELDLQELAGVLMREAAKMPGPSFPIGEGGLKKKVERLVKAGA
                     VAVVTVRGPRSFLSPEERDADELPKLEGGEGLLGEALPVPVVQMKWKSVDRLLGGRKL
                     SKLQAQIDADRAPHSQPLGRELALSVALEPVNVQVPNVLASLPGGDLSQEIVMIGAHY
                     DHIGVAGAGQCGEAKDGDKVDKICNGADDNASGTAMLLELARAWKQSGLRPRRTIVFT
                     HFAGEELGVLGSKALAEAPPFDLKRVVAMVNLDMIGRLGPKGLAVGGLGSSDGWMPLL
                     DAVGPAGLEVLYESSVATRSDHASFYRKDIPVLFFFTGVHSDYHRPGDHSDKINLTGM
                     SAIGEIVAGVLVQLGDGYKVPWKPPGPKGGLSQGLPGSDPATVVKRVQAAGTSAATAQ
                     DGAAK"
     gene            2582..>3489
                     /locus_tag="VGB85_04780"
     CDS             2582..>3489
                     /locus_tag="VGB85_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0133366.1"
                     /translation="MQAGLLAGIGLAFVGGAEPPTVDAAPVPCQKEGNCTFKKPLFGI
                     ILDNSSSMNELFDADSTRWEGAVDAITQAISYDNGYIAENFILGLIRFGHDPAPQTPG
                     TKIPADSSGLVDGQKLDVPWYDDAAPNKPYIECTNGDAILAALEAVGAPSNGSKTGIG
                     TWTKGAMDFAAAYIAKAKADHPLDADKRAAILLVLTDGKWTDPTGVNQLKPDNQNPKI
                     TAGSLWMQQMIPTYVVAIGDAEGQGFANELAAAGGTTAAIDAGNPQELIDALTAVIVK
                     IQKDVVAPICTPGLPRIMVLLDASSS"
BASE COUNT          538 a         1279 c         1164 g          508 t
ORIGIN      
        1 caggttcgcc gcgcgggcgc cggcccgccg cagcgcctcc gggtcgagca gggcgtcggg
       61 gtgcaggtcg acgcgaacgc gcagcgggcc cgacatgact acttcgcagc cccgtcctgc
      121 gccgtcgcag cgctcgtccc ggccgcctgg acccgcttga cgaccgtcgc cgggtcgctg
      181 cccggcagcc cctggctgag cccgcccttg ggccctggtg gcttccaggg caccttgtag
      241 ccgtcgccga gctgcaccag cacgcccgcg acgatctccc cgatcgcgct catgccggtg
      301 aggttgatct tgtcgctgtg atcgccgggg cggtggtagt cgctgtgcac gccggtgaag
      361 aagaacagca ccgggatgtc cttgcgataa aagctcgcgt ggtcgctgcg ggtcgccacg
      421 ctgctctcgt agagcacctc gaggcccgcg ggtccgaccg cgtcgagcag cggcatccag
      481 ccgtcgctcg acccgagccc gccgaccgcc aggcccttgg gtccgaggcg gccgatcatg
      541 tcgaggttga ccatcgcgac cacgcgcttg aggtcgaagg gtggcgcctc ggccagcgcc
      601 ttcgagccga gcacgccgag ctcctcgccg gcgaagtgcg tgaacacgat cgtgcgtcgc
      661 ggtcgcagcc cgctctgctt ccacgcgcgg gccagctcga gcagcatcgc cgtgccgctg
      721 gcgttgtcgt ccgcgccgtt gcagatcttg tcgaccttgt cgccgtcctt tgcctcgccg
      781 cactgcccgg ccccggcgac gccgatgtga tcgtagtgcg cgccgatcat cacgatctcc
      841 tgcgagagat cgccgccggg caggctcgcc agcacgttcg ggacctgcac gttgaccggc
      901 tcgagcgcga ccgacagcgc cagctcgcgc ccgagcggct gggagtgcgg cgcccggtcg
      961 gcgtcgatct gcgcctgcaa cttcgacagc ttgcgcccgc cgagcaggcg gtcgacgctc
     1021 ttccacttca tctgcacgac cggcaccggc agcgcctcgc cgagcagccc ctcgccgccc
     1081 tcgagcttcg gcagctcgtc cgcgtcgcgc tcctccggcg aaagaaagct ccgcggccca
     1141 cggaccgtga ccacggcgac ggccccggcc ttgaccagcc gctcgacctt cttcttgagc
     1201 ccgccctcac cgatcgggaa gctcgggccg ggcatcttcg ccgcctcgcg catcaacacc
     1261 ccggcgagct cctgcaaatc gagctcgacc gtcgcgacgt cctgcggcag cggccacagc
     1321 tcgtcggccg cgagctgcgg catgaacggc cgcatcagcg cgtatagccg cttctgggcg
     1381 ctcgtgtgca gcttcgtcag gccccgcagg tcgccggcgg ccttgagcgg cgccgcggcg
     1441 tccgtgaagc gctgggactc ctcttgcagg cgcatgaaca gggtcagcgg gtccggccgg
     1501 cccggcacct cgagcagcac caacgcgacc ttgcccttga ggtcgatgcc cgcgaggtcg
     1561 tcgtacggcg ccgcgacgag ctcgctctgc ttgcccgcgc ccggctcacc ggcgcccggc
     1621 ggcttcggcg gctccgactg cgcggcgtag ccggcgaaca ccagctcgcc tcgcaccgcg
     1681 cccgagccgc tctgcgccac gaccgcgaac tccggcgcgg ccagcgtcgt cggcttggcg
     1741 gcggccccgg gcttgaacag ctccaggctc gccgcctcgc gcaggcgggc gccggtgcgc
     1801 agcgggaagt cgatcatgaa gtcggcgccg accggcgaga ggccgagctc tgcgtggcgc
     1861 ttcgccagca gctcggccgc gcgacgcagg tccgggctca gcgtgtagcg cccgttgaag
     1921 tcgtccgccg ccagcgtggc gatgtccgcg gacatgcgcg ccgcgtcgag ctcgagccgc
     1981 ggcggacgca ccggcgccgg tgcgaccgcg ggcgggcgcg gtggtgccgt gttcgcgacc
     2041 tcgcggtgac acgcgagcgc cagcaggacc ggcgaaatcg ttgacaggac actgaccgcg
     2101 cgcatcgtcg gcgcgtggac atcgggccgc gagcgaggcg agtgcacgga gcgacggggc
     2161 atgtccccgc gttataaccc tggcacctcg tcgcggccgg cgatctccct gcttcgaagt
     2221 ggagggaatc gttgaagtgc gctcacgcaa ggtgaagacc agtgcacgtg cgttgcttca
     2281 cagattcttg ccatgttcgt acgccacaga cgcagaaggg accacgggcg aggaagatcc
     2341 aggtcgggcg ttcgaccgcg gaccagcggc gaggtcgagg acccgccaag ggccgcggac
     2401 agcggttagc ccgtgctcta ccgcagtatt gacgccccca ggcaactggc gtaacgtccg
     2461 ggctcaacga gtaaagcgag ttcgcacgcc aacgtgcccg cgagctctca actactagca
     2521 agcaaggatt acaaaagcga tgaagcgcct cagtttgatt ggttcatgga agcggattgg
     2581 cgtgcaagcg ggactcctgg cgggtatcgg gctcgctttc gtcggcggcg cggagccccc
     2641 gacggtcgac gcggcgcccg tcccctgtca gaaggagggc aactgcacct tcaagaagcc
     2701 gctgttcggc atcatcctcg acaactcgag ctcgatgaac gagctgttcg acgccgacag
     2761 cacgcgctgg gaaggcgccg tcgatgcgat cacccaggcg atttcctacg acaacggcta
     2821 catcgctgag aacttcattc ttggcctgat ccgtttcggt cacgatccgg ccccgcagac
     2881 cccgggcacc aagatcccgg cggacagctc cggcctggtc gacggtcaga agctcgacgt
     2941 gccctggtac gacgacgccg ccccgaacaa gccgtacatc gagtgtacca acggcgacgc
     3001 gatcctcgcg gcgctcgagg ccgtcggcgc gccgtccaac ggcagcaaga ccggcatcgg
     3061 cacctggacc aagggcgcga tggacttcgc cgcggcgtac atcgccaagg ccaaggccga
     3121 ccacccgctg gacgcggaca agcgcgccgc gatcctcctc gtcctcacgg acggcaagtg
     3181 gaccgacccg acgggcgtca accagctcaa gccggacaac cagaacccga agatcaccgc
     3241 tggctccctg tggatgcagc agatgatccc gacctacgtc gtcgccatcg gcgacgccga
     3301 gggtcagggc ttcgccaacg agctggccgc cgccggtggt accaccgccg cgatcgacgc
     3361 tggcaacccg caggagctca tcgacgccct cacggccgtc atcgtgaaga tccagaagga
     3421 cgtcgtcgcg ccgatctgca ccccgggcct gccccgcatc atggtgctcc tcgacgcctc
     3481 ctcgtcgat
//
LOCUS       DASWNJ010000263         2262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_181443, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000263 DASWNJ010000000
VERSION     DASWNJ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181443"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            785..>2262
                     /locus_tag="VGB85_04785"
     CDS             785..>2262
                     /locus_tag="VGB85_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0133367.1"
                     /translation="MMTLSRLAISSGDQLLPRRASVSTLAQLLIEAAERAPESRLICL
                     DLNGREEAMTYGELLLRSQRCLGELQAAGVGVGDRLLLQLPDGAGFFTALWACFLGGI
                     IPVPVRITAVIEPGHPGMARLLDAWRTLDRPPILTDDATIAEGFRRVAATEAGAGMRV
                     LTLTGRADHEPGRIHTGAPAAVALMLLTSGSTGGPKAVMLGERNLLSMSASTIQQHGF
                     TADEVTLNWMPLDHVGALTFLHLMAVDLGCSQLHVPTEYVLRAPLRWLELSAAHRATI
                     SWAPNFAFSLINDQAPALAARPCDLSRLRFLVNGGEQVGYRTMHRFITLLQQYGLPPG
                     ALRPSFGMSETCAGITWSSGLRLTDLADEQVYLSLGRPGPGAALRITDEQHQTLAEGE
                     IGRLQIKGPTVTCGYYGDPERTREVLDADGWFTTGDLGFIRDGELVITGREKHDIILH
                     GVNHAAHEIEAMVEEVAGVAAAGSVAFAVREAARELEVLAVV"
BASE COUNT          362 a          762 c          794 g          344 t
ORIGIN      
        1 aagatgctcg gccaccgcaa ctaccacacg acccagcgtt acgcgcacat tgccgacacc
       61 atcttgcgcg acgccgtgag cctgacgagc aagacgaccg gccgcgcaac caggaatgga
      121 agagaccttg cagggcgacg gcgcaggccc cggcccgctg gcgcacccat acgcaggtaa
      181 gcgtccgatc tatcaggtcg tgacggcgac agcccggacg catatactcg gcgccacgaa
      241 gtccgactgc gaatacgatg agaacggccg gatccccgca tatcatcggt cgccgagctc
      301 accgcggtcg ccaccacccg accaggaccg acaaaggtcg tcgtcgcgcg ccggtgtcat
      361 ggaggcgatg ccgaggtcgg tccgcggtat gccgaggccc tgcgcctccg cgtcggggtc
      421 ggcttgcgcg agcgcgcgca tgagctcgac gcgccgggcc ttgaggaagc gcggatagat
      481 gctgtggtta gatcacccca tcttatcggt gtcggcgtag attcacccgc tgctgcaatg
      541 cgagctgcac ggggcgacca gggcctggag agccggggcg gatgagcgag cacctgcgag
      601 gctaccgaag cctggcgctc ctgcagcctc gtaagtgagt tagcccgagg ctttccggcg
      661 cgaggtgctg actcgccact ttacaggacc tttcacggct gcgcggcgcg gtggatcttc
      721 gcggaccaag cggttatgag ggacagcctc cgcgatgcgg atgctcgtgt gatctcagga
      781 gcccatgatg acactgtcaa gattggcgat atcgagcggt gatcaattgc ttccgcggcg
      841 ggcgagcgtc tcgacgctcg cgcagctgct cattgaagcc gccgagcggg ccccggagag
      901 ccggttgatc tgcctcgatc tgaacgggcg ggaagaggcg atgacctacg gggagctgct
      961 gctgcgcagc cagcgatgcc tcggagagct acaggccgcg ggcgtcggcg tcggcgaccg
     1021 gctcctgctg cagctccccg acggcgccgg cttcttcacc gcgctatggg cttgtttcct
     1081 cggtggcatc atcccggtcc cggtgcggat cacggcggtg atcgagcccg ggcacccggg
     1141 catggcgcgg ctcctcgacg cgtggcgtac gctcgatcga ccgccgatct tgaccgacga
     1201 cgcgacgatc gccgagggct ttcgtcgggt cgccgcgacc gaagcggggg ccggaatgcg
     1261 cgtgctgacg ctgaccgggc gcgccgacca tgagccgggt cgcatacaca caggcgcccc
     1321 cgcggcggtg gcattgatgc tgctgacctc cggcagcaca ggggggccga aggccgtgat
     1381 gctcggcgag cgcaacctgc tgagcatgag cgcgagcacg atccagcaac acggcttcac
     1441 ggccgacgag gtgacgctca actggatgcc gctcgaccac gtcggcgcgt tgacgtttct
     1501 ccacctcatg gcggtcgacc tcggctgctc gcagctccac gtcccgaccg aatacgtgct
     1561 gcgcgcgccg ctgcgctggc tcgagctgag cgctgcgcac cgggcgacga tctcgtgggc
     1621 gccgaacttc gcgttctcgc tgatcaacga ccaggcgccg gcgctcgcgg cccgcccctg
     1681 cgatctttcg cgcctgcgct tcctcgtcaa cggcggcgag caggtcgggt atcggacgat
     1741 gcaccgattt atcaccctgc tgcagcagta tggcctgccg ccgggggcgc tgcggccgag
     1801 cttcggcatg tccgagacct gcgccgggat cacgtggtcg agcggcctgc gcctgacgga
     1861 cctcgcggac gagcaggtct acctctcgct cgggcgcccg gggccgggag ccgcgctgcg
     1921 gatcaccgac gagcagcatc agacgctcgc cgagggcgag atcgggcggc tgcagatcaa
     1981 gggcccgacc gtgacgtgcg gctattacgg cgaccccgag cgcacccgcg aggtgctcga
     2041 cgccgacggc tggttcacca ccggcgacct cggcttcatc cgcgacggcg agctggtgat
     2101 caccggccgg gagaagcacg acatcatcct ccacggcgtc aaccacgccg cgcacgagat
     2161 cgaggcgatg gtcgaggagg tcgcgggagt cgcggcggcg ggcagcgtgg cgttcgcggt
     2221 ccgtgaggcg gcgcgcgagc tcgaggtgct ggcggtcgtg tt
//
LOCUS       DASWNJ010000264         1990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_111086, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000264 DASWNJ010000000
VERSION     DASWNJ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111086"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..966
                     /locus_tag="VGB85_04790"
     CDS             289..966
                     /locus_tag="VGB85_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0133368.1"
                     /translation="MVTTQGDTLTAPLRVMVVDDHQTFADLLSLALSFEPDLECVGTA
                     GSAAEAVAMAAELRPNVVVMDIEMPRQDGLAATRRLREVLPDIVIVVVTAHRDPQWVL
                     RATQAGASAFVPKNGSLPEMLDVLRRARNGGMLVAASAFGSTSAPPERLPAAPRVDLT
                     QRERDVLHCLNRGMAPKAIARVLGISLHTCRGYVKSLLSKLGVSSQLEAVVTAQRLGL
                     IERSDGG"
     gene            999..>1990
                     /locus_tag="VGB85_04795"
     CDS             999..>1990
                     /locus_tag="VGB85_04795"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY0133369.1"
                     /translation="MRSSLITYLGAAALVLVIVAVGAILVGRQVAQDEALHDAERVAQ
                     RTANLVIAPLLGDIMNGDASRRPELDRAVAIRLRDGSISELNVWDRTGTIIYSDDAQD
                     IGERLALPEEVAAAIDEGAVRSNVTTEPETGPLPGGEQRFVEVYAPLRLPGQAPLAFE
                     IYVSYDRVENTANNLVLRIVPLAVGALLLLQLIQTPITVSLARRVAGHEAERSALLER
                     ALSASERERRQIAADLHDGVVQDLAGAGYALAALQRRLDDPAGKNVDHVGRVVRGAVE
                     SLRRLMVDIYPPDLSGAGLPGAIDDLAEPLRRAGTTVHVRAEPLPGADSDVAATL"
BASE COUNT          268 a          745 c          715 g          262 t
ORIGIN      
        1 catccgttct gcctggtgac ggcatgatca cgcggtgttc accggccacc cggtcgaggg
       61 tcacctcatc gggtgggcgc ggcccctgac cgatgtgctc gcggccacag gtgaccacag
      121 ttggcgacat gccgaaccgg gataacggcg cgagcccgtc actcgcgcgg gcggccgtct
      181 gtcactgtgc gtagtgccgg acgtgggaac gacgaccaat ccgtgggttg taacgccacg
      241 aatgacggat gcgagttcgg cggcaggtag ccgggaggcg tggaggccat ggtgacgacg
      301 cagggcgaca cgctgacggc tccgctgcgc gtcatggtcg tcgacgacca ccagacgttc
      361 gccgacctgc tctcgctcgc cctgtccttc gagcccgacc tcgagtgcgt gggcacggcc
      421 ggcagcgccg cggaggccgt ggcgatggcc gccgagctcc ggccgaacgt cgtcgtcatg
      481 gacatcgaga tgccgcgcca ggacggcctg gcggcgacgc ggcggctgcg cgaggtcctg
      541 ccggacatcg tcatcgtggt ggtcaccgcg caccgcgacc cgcagtgggt gctgcgggcg
      601 acgcaggcgg gtgcgtcggc gttcgtgccc aagaacggca gcctgcccga gatgctcgac
      661 gtgctccgcc gggcccgcaa cggcggcatg ctggtggccg cgtcggcgtt cgggtccacc
      721 tccgcgccgc ccgagcgcct ccccgccgcg cccagggtcg acctcaccca gcgcgagcgc
      781 gacgtcctgc actgcctcaa ccgggggatg gcccccaagg ccatcgcccg cgtgctcggc
      841 atcagcctgc acacctgccg gggctacgtg aagtcgctgc tgtccaagct gggggtgagc
      901 tcgcagctgg aggccgtcgt gaccgcccag cggctcggcc tcatcgagcg ctccgacggg
      961 ggctgacgat gcgggcaggg gtccggacga atccgcaggt gcgcagctcg ctgatcacct
     1021 acctcggcgc cgccgccctc gtgctggtca tcgtcgccgt cggcgccatc ctcgtcggcc
     1081 gccaggtcgc ccaggacgag gccctgcacg acgccgagcg ggtcgcgcag cgcaccgcga
     1141 acctggtgat cgccccgctg ctcggcgaca tcatgaacgg cgacgcgtcc cggcgccccg
     1201 agctcgaccg cgccgtggcc atccggctcc gcgacgggtc gatcagcgag ctgaacgtct
     1261 gggaccgcac cggcacgatc atctactcgg acgacgccca ggacatcggc gagcgcctcg
     1321 cgttgcccga ggaggtggcc gccgcgatcg acgagggcgc cgtccgatcc aacgtgacca
     1381 ccgagcccga gacggggccc ctgcccggcg gcgagcagcg gttcgtcgag gtgtacgcac
     1441 cgctgcggct gcccggacag gccccgctcg ccttcgagat ctacgtgagc tacgaccggg
     1501 tcgagaacac ggcgaacaac ctggtgctgc ggatcgtgcc gctggcggtc ggcgcgctgc
     1561 tgctgctgca gctcatccag accccgatca ccgtctcgct cgcccgccgc gtcgccgggc
     1621 acgaggccga gcgcagcgcc ctgctcgagc gcgccctgtc ggcctcggag cgggagcgcc
     1681 ggcagatcgc cgccgacctg cacgacgggg tcgtccagga cctcgccggg gccgggtacg
     1741 cgctcgccgc cctgcagcgg cgcctcgacg acccggccgg gaagaacgtc gaccacgtgg
     1801 gccgcgtggt gcgcggggcc gtcgagtcgc tgcgccggct catggtcgac atctacccac
     1861 cggacctgag cggggcgggc ctgccgggcg cgatcgacga cctggccgag ccgctgcgcc
     1921 gcgccgggac caccgtgcac gtgcgcgccg agcccctgcc cggcgccgac tccgacgtcg
     1981 cggccaccct
//
LOCUS       DASWNJ010000265         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_357513, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000265 DASWNJ010000000
VERSION     DASWNJ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357513"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VGB85_04800"
     CDS             <1..798
                     /locus_tag="VGB85_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133370.1"
                     /translation="AHLLTAQYCVDGAGNPAACDTGAPYQAGVLTTRAYLIANASRFN
                     LRRARRMMFIFSCNVYPMDPLLQPPISKEQLIPMFRALTQEEQTEPGAMSGFGNGSAC
                     YSCHSQFSAHAQLFVRFSQDGLWRADATGLQDPDNELGRSTNGLYASHFVSPVSAPLE
                     VSQMFGVPVKGLSDAARVLTEGPGFYTCAARNVLEYSFGMSETEAVSIERDLLDELAA
                     LATARDLRLRPETAGTPEGGPTFGDLFVVALTHPRVLQVVLGPPDPA"
     gene            835..2397
                     /locus_tag="VGB85_04805"
     CDS             835..2397
                     /locus_tag="VGB85_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133371.1"
                     /translation="MQRPVRSASRKPFIETLHPLQRRGFMKMLGVALAVPTVPVAVRH
                     AARAFAFGEARAEEMAKPPINFIEIDLRDQWDFGQVFVAPGLATNVNLKRGESGRMCA
                     LYYGADQLTHVPDRNVYLTPLSLPLMPHLDTIAMIETCELGIGDIHGHEASNPQRSPG
                     RSGVPGPGRGDMSANEPVPFVPGNSLHYSSTPTPAALHNHWIKQQGVLVKNGVAFKGI
                     SREHTAYHYGAGLDGSELDRKQTVAALMAGFPDKLEDYNVVPSPQQAEALVKVLSAVD
                     DRFLGRHKYEVTASGQHLANLGELSPLLYTGEPKLISLPLSPEEVAYWQMGVPDQVGN
                     PPGLKANIWEQCAYAFKLIAGAQCSTVALEFDFIDWHDSRPQNIMDTLTLQTVLPLTR
                     LIESLKLAGLYENTLIALYTLDGGRAPAAGSSGGEGKSGVMLAGGMIQGGYYGDVGVA
                     GDDGDGHVYSYSAPNPDTGAPMPGVTDNSGRLAGGHVWRTVMKALGVPDTLAASFPDV
                     ANYKPLDWMLRA"
BASE COUNT          383 a          942 c          764 g          343 t
ORIGIN      
        1 gcccacctgt tgaccgcgca gtactgcgtc gacggggccg gcaacccggc cgcgtgtgac
       61 acgggcgccc cgtaccaggc cggcgtgctg acgacccgcg cctatctgat cgccaacgcg
      121 agccgcttca acctgcgtcg cgcccggcgc atgatgttca tcttcagctg caacgtgtac
      181 ccgatggacc cgctgctgca gccgccgatc agcaaggagc agctgatccc gatgttccgc
      241 gcgctgaccc aggaggagca gaccgagccc ggcgccatga gcggcttcgg caacggcagc
      301 gcctgctact cgtgtcacag ccagttcagc gcgcacgccc agctgttcgt ccgcttcagc
      361 caggacggcc tgtggcgcgc cgacgcgacc ggcctgcagg accccgacaa cgagctcggg
      421 cgcagcacca acggcctcta cgcctcccac ttcgtgagcc ctgtctcggc cccgctcgag
      481 gtctcgcaga tgttcggcgt gcccgtgaag ggcctgagcg acgctgcccg cgtgctgacc
      541 gagggcccgg gcttttacac ctgcgccgcg cgcaacgtgc tcgagtacag ctttggcatg
      601 agcgagaccg aggccgtctc gatcgagcgc gacctgctcg acgagctcgc ggcgctggcg
      661 accgcccgcg acctgcggct gcggcccgag accgccggca cccccgaggg cggcccgacc
      721 tttggtgacc tgttcgtcgt cgccctcacg cacccgcgcg tgctgcaggt cgtgctcggc
      781 ccgcccgacc ctgcctagag aacccgtcgt ctcgccgtcc cattcggaag ccttatgcaa
      841 cgacccgtac gatccgcctc gcgcaagccg ttcatcgaga ccctgcaccc gctgcagcgc
      901 cgcggtttca tgaagatgct cggcgtcgcg ctcgcggtgc cgaccgtccc cgtcgccgtc
      961 cgccacgccg cccgcgcctt cgcgttcggc gaggccaggg ccgaggagat ggccaagccc
     1021 ccgatcaact tcatcgagat cgacctgcgc gatcagtggg acttcggcca ggtgttcgtc
     1081 gccccgggcc tggcgaccaa cgtcaacctc aagcgcggcg agtcgggtcg catgtgcgcg
     1141 ctctactacg gcgccgatca gctgacccac gtgcccgatc gcaacgtgta cctcacgccg
     1201 ctgtcgctgc ccctgatgcc acacctcgac acgatcgcca tgatcgagac ctgcgagctc
     1261 ggcatcggcg acatccacgg ccacgaggcg agcaaccccc agcgctcgcc cggccgtagc
     1321 ggcgtgcccg gacctggccg cggggacatg tccgccaacg agcccgtgcc cttcgtgccc
     1381 ggcaactcgc tgcactacag ctcgacgccg accccggccg cgctgcacaa ccactggatc
     1441 aagcagcagg gcgtgctcgt caagaacggc gtcgcgttca agggcatcag ccgcgagcac
     1501 accgcctacc actacggcgc cggcctcgac ggctccgagc tcgaccgcaa gcagaccgtc
     1561 gccgcgctga tggccggctt ccccgacaag ctcgaggact acaacgtcgt gccctcgccg
     1621 cagcaggccg aggcgctggt caaggtgctc agcgccgtcg acgaccgctt cctcgggcgg
     1681 cacaagtacg aggtgaccgc gagcggccag cacctcgcca acctcggcga gctgtcgcct
     1741 ctgctctaca ccggcgagcc caagctgatc agcctcccgc tttcaccgga ggaggtcgcc
     1801 tactggcaga tgggcgtgcc cgaccaggtc ggcaacccgc ccggcctcaa ggccaacatc
     1861 tgggagcagt gcgcctacgc cttcaagctg atcgccggcg cccagtgcag taccgtggcg
     1921 ctcgagttcg acttcatcga ctggcacgac tcgcgcccgc agaacatcat ggacacgctc
     1981 acgctgcaga cggtgctgcc gctgacgcgc ctgatcgaga gcctcaagct cgccggcctg
     2041 tacgagaaca cgctgatcgc cctgtacacc ctcgacggcg ggcgggcccc ggccgccggc
     2101 tcctcgggcg gtgagggcaa gagcggcgtg atgctcgcgg gcggcatgat ccagggcggg
     2161 tactacggcg acgtcggcgt cgccggcgac gacggcgacg gccacgtcta cagctacagc
     2221 gcccccaacc cggacaccgg cgcgccgatg cccggcgtca ccgacaacag cgggcgcctc
     2281 gccggcggcc acgtgtggcg caccgtcatg aaggccctcg gcgtccccga caccctcgcc
     2341 gccagcttcc cggacgtcgc caactacaag ccgctcgact ggatgctgcg cgcctgatcc
     2401 cgatcgcgct cagggcactg gcgggtccat gc
//
LOCUS       DASWNJ010000266         2143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_40535, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000266 DASWNJ010000000
VERSION     DASWNJ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40535"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1706)
                     /locus_tag="VGB85_04810"
     CDS             complement(<1..1706)
                     /locus_tag="VGB85_04810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005416 - amino acid:monoatomic cation
                     symporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid carrier protein"
                     /protein_id="HEY0133372.1"
                     /translation="MDALRTGLNDWLTAINAVIWHDSVLYIVLAVGLLFTIWSGFCQY
                     HALTHGVRVITGRYDDKADPGAISHFQALSAALSATVGLGNIGGVAVAIALGGPGAVF
                     WMWVVGLAGMALKTTEVTLSMLYRRTDNPDEPSGGPMWVVDDGLRQRSPRLAWLGRLL
                     GVLFCITLMISTITGGNMFQAWNVGVVTEESFGVPQAVVGAILAVLVGLVILGGIKRI
                     GAFAGAIVPVMCGLYLVAALYVLAYNITAVPAILKLIVLSAFSPAEASGAFVGGTFGM
                     AFLWGMKRALFSCEAGQGSSPIAHCAAKTTEPVREGVVAGLEPFIDTIVVCTLTSLVV
                     LSSGAWNRGGEATLPAPPAFVSVEPGVWALPNTALPAKTPEALAITGTDWRVGDDVFL
                     MARYGAVDRGSGTDLHRVPGKVVAGEGDRLHVAWTSISPGPADSSADPASALAEAPAL
                     HSAEVYKDYVGAALTSHAFDRVAPGLGKWLVAVASWLFAVSTMISWAYYGEQGMVYMA
                     GRRSVLPYKLFYCASTFIACVGIVSTEEELDNITALGTGVMLWVNIPIMLLFGGAAMG
                     AYR"
     gene            complement(1771..>2143)
                     /locus_tag="VGB85_04815"
     CDS             complement(1771..>2143)
                     /locus_tag="VGB85_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133373.1"
                     /translation="KVYGPPTKPLTGALPTPDTDGKVEFPAAKNMEVSWEFADLIARV
                     NVLRYGELVTVGERVEVPHGLRPDAPRFVAGQTPPLAPVSVPMPALPGGGPASAPTAP
                     SVPSAPSAPSVPLAPSSPAGP"
BASE COUNT          344 a          701 c          824 g          274 t
ORIGIN      
        1 cggtaggcgc ccatcgcggc gccgccgaac agcagcatga tcgggatgtt gacccacagc
       61 atcacgccgg tgccgagcgc cgtgatgttg tcgagctcct cctcggtcga gacgatgccg
      121 acgcaggcga tgaaggtgct ggcgcagtag aacagcttgt agggcagcac gctgcgccgc
      181 ccggccatgt agaccatgcc ctgctcgccg tagtacgccc acgagatcat ggtcgaaacc
      241 gcgaacagcc acgaagcaac agcgacgagc cacttgccga ggcccggcgc gacgcggtcg
      301 aaggcgtggc tggtcagcgc cgcgccgacg tagtccttgt agacctcggc gctgtgcagc
      361 gcgggcgctt ccgcgagggc gctggcgggg tcagcggagg agtctgcggg gcccggggag
      421 atcgaggtcc acgcgacgtg gaggcgatct ccctcgccgg cgacgacctt gccggggacg
      481 cggtgcaggt cggtgccgct gccgcgatcg accgcgccgt agcgggccat caggaacacg
      541 tcgtcgccga cgcgccagtc ggtgccggtg atagcgaggg cctcgggggt cttggccggc
      601 agggcagtgt tcggcagggc ccagacgccg ggctcgacgg agacgaaggc gggcggggcc
      661 ggcagcgtcg cctcgccgcc gcggttccag gcgccgctcg agagcaccac gagcgaggtc
      721 agggtgcaga cgacgatcgt gtcgatgaag ggctcgaggc cggcgacgac gccctcacgc
      781 acgggctcgg tggtcttggc ggcgcagtgg gcgatcggcg aggagccttg gccggcctcg
      841 caggagaaca gggcccgctt catgccccac aggaacgcca tgccgaaggt gccgccgacg
      901 aaggcgccgc tcgcctcggc cgggctaaac gcgctgagca cgatcagctt gaggatcgcc
      961 ggcacggcgg tgatgttgta ggcgagcacg tagagggcgg cgacgaggta caggccgcac
     1021 atcaccggca cgatcgcgcc cgcgaaggcg ccgatgcgct tgatgccgcc gaggatcacg
     1081 aggccgacga gcaccgcgag gatcgccccg acgaccgcct gcgggacgcc gaagctctcc
     1141 tcggtgacga cgccgacgtt ccaggcctgg aacatgttgc cgccggtgat cgtggagatc
     1201 atcagcgtga tgcagaacag cacgccgagc aggcgaccga gccaggcgag ccgcgggctg
     1261 cgctggcgca ggccgtcgtc gacgacccac atcgggccgc cgctcggctc gtcggggttg
     1321 tcggtgcggc gatacagcat cgacagggtc acctcggtgg tcttgagggc catgccggcg
     1381 aggccgacga cccacatcca gaacaccgcg ccgggcccac cgagggcgat cgccacggcg
     1441 acgccgccga tgttgccgag cccgacggtc gccgagagcg cggcggacag ggcctggaag
     1501 tggctgatcg cgccggggtc ggccttgtcg tcgtagcgcc cggtgatcac gcggaccccg
     1561 tgggtcagcg cgtggtactg gcaaaagccg ctccagatcg tgaacagcag gccgacggcc
     1621 agcacgatgt agagcacgct gtcgtgccag atcaccgcgt tgatcgcggt caaccagtcg
     1681 ttgaggcccg tgcgcagcgc gtccatttcg tgcccctcgc cccgggcgcc gggatgatgc
     1741 aggggccggc caccgagcgc aacgccgatg tcaggggccg gcgggggatg atggggcgag
     1801 tgggacggat ggggcagatg gggcagatgg gacagatggg gcagtgggcg cagatgcagg
     1861 gccccccccg ggcagggccg gcatcggcac gctgacgggt gccagcggcg gggtctggcc
     1921 ggcgacgaag cgcggggcgt cggggcgcag gccgtgcggg acctcgacgc gctcgccgac
     1981 ggtgaccagc tcgccgtagc gaagcacgtt gacccgcgcg atcaggtcgg caaactccca
     2041 gctgacctcc atgttcttgg cggccgggaa ctcgaccttg ccgtcggtgt ccggcgtcgg
     2101 cagggcgccg gtcagcggtt tggtcggcgg gccgtagacc ttg
//
LOCUS       DASWNJ010000267         2335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_40585, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000267 DASWNJ010000000
VERSION     DASWNJ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40585"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VGB85_04820"
     CDS             complement(<1..506)
                     /locus_tag="VGB85_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133374.1"
                     /translation="MNKFVSLAIASALWTFTTQAAPPSSTPAKAPPTTTPTPARVGPV
                     YLVGGELHVGDGTIIKDAVIAMDGGKFTIVGGAEARARLLDESSAIDLRGKVITPGLF
                     ASDTQLGLVEIDLEAATRDDGKDTEHPIRAAHDAATALNAESGLIPIQVVEGVTTAAV
                     VPTGGLISG"
     gene            complement(503..2026)
                     /locus_tag="VGB85_04825"
     CDS             complement(503..2026)
                     /locus_tag="VGB85_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY0133375.1"
                     /translation="MIKRRPVLLLVAVSAALACGKQGETRPPEPAVAKAPASAKSEPR
                     GPGHKTLLRGAAVMTATGKIYARADLLVEDSKIVAVGENLAVPEGATVVDLTGKTLTP
                     GIIDTHSHIGVYAAPGLTAHSDGNEAVAPNTAGVRAADGYFPQDPQIDRARAGGITTM
                     QILPGSANLFGGRTFTVRLHPGARSSDDARFPGAPEGLKIACGENPKRVYGQKSGPQT
                     RMGNVAGHREAFQRAAEYQRTWAKYHKDMSEWTAEKDKPKKSSEDHDKNKGKKPDKPQ
                     PPARDFALETLVKVIRGEILVHNHCYRADEMVIMLDLAATYGFKIRSFHHATDAYKIA
                     DRLAAAGTSASVWADWWGFKAESFDGIRENAALLTKAGARAIIHSDSPVGIQRLNQEA
                     AKAMHAGRRAGIEITDDQALRWITANAAWALGIDDRTGSLEPGKLADLVVWNTTPFSV
                     YAVAEQVWIGGELVYQRGKLEPRTDFELGYLEGDLDRSAPPPATTSTPVAAPPGGAR"
     gene            2123..>2335
                     /locus_tag="VGB85_04830"
     CDS             2123..>2335
                     /locus_tag="VGB85_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133376.1"
                     /translation="MTRSLARSPARLIGFSAAFLLTVATGACLPERGGVPDKAAVAAA
                     GNNGPAAKAAGRVAQPTPRPQPEVAPA"
BASE COUNT          282 a          776 c          888 g          389 t
ORIGIN      
        1 ccggagatca ggccgccggt cgggacgaca gcggccgtgg tgacgccctc gacgacctgg
       61 atcgggatca ggcccgactc ggcgttgagc gccgtggcag cgtcgtgcgc ggcgcggatc
      121 gggtgctcgg tgtccttgcc gtcgtcgcgg gtcgcggcct cgagatcgat ctccaccagc
      181 ccgagctgcg tgtcgctggc gaacagcccg ggcgtgatca ccttgccgcg caggtcgatc
      241 gccgacgact cgtcgagcag gcgcgcccgc gcctcggcgc cgccgacgat cgtgaacttg
      301 ccgccgtcca tcgcgatcac agcatcttta ataattgtgc cgtcgccgac gtggagctcg
      361 ccgccgacga ggtacaccgg gccgacgcgc gccggggtcg gggtcgtggt cggtggagcc
      421 ttggcgggcg tcgagctcgg cggcgcggcc tgggtggtga acgtccacag cgcgctggcg
      481 atggcgagag agacgaactt gttcatcggg cacctccggg gggcgctgca acaggtgtgg
      541 aggtcgtcgc gggtggtggg gcgctgcggt cgaggtcacc ctcgagatag ccgagctcga
      601 agtcggtgcg cggctcgagc ttgccgcgct gatacacgag ctcgccgccg atccacacct
      661 gctcggcgac ggcgtagacg ctaaacgggg tcgtgttcca gacgacgagg tcggcgagct
      721 tgccgggctc gagcgagccg gtccggtcgt cgatgccgag ggcccacgcg gcgttggcgg
      781 tgatccagcg cagggcctgg tcgtcggtga tctcgatgcc cgcgcggcgg ccggcgtgca
      841 tcgccttggc ggcctcctga ttgaggcgct ggatgccgac cgggctgtcg gagtggatga
      901 tcgcgcgggc gccggccttc gtcagcagcg cggcgttctc acggatgccg tcgaaggact
      961 cggccttaaa accccaccag tcggcccaca cggacgcgga ggtgccggcg gcggcgaggc
     1021 ggtcggcgat cttgtaggcg tcggtcgcgt ggtgaaagct gcggatcttg aagccgtagg
     1081 tggccgcgag gtcgagcatg atcaccatct cgtcggcgcg gtagcagtga ttgtgcacga
     1141 ggatctcgcc gcggatcacc ttgacgaggg tctcgagcgc gaagtcccgc gcgggcggct
     1201 gcggcttatc gggcttcttg cccttgttct tgtcgtggtc ctcgctcgac tttttgggct
     1261 tgtccttctc ggctgtccat tcggacatgt ccttgtggta cttcgcccag gtgcgctgat
     1321 actcggcggc cctttgaaac gcctcgcggt ggccggcgac gttgcccatg cgcgtctgcg
     1381 ggccggactt ttgcccgtag acgcgcttgg ggttctcgcc gcaggcgatc ttgaggccct
     1441 cgggggcgcc ggggaagcgg gcgtcgtcgg agctgcgggc gccggggtgc aggcgcacgg
     1501 tgaaggtgcg gccgccgaac aggttggccg agccaggcag gatctgcatg gtcgtgatgc
     1561 cgccggcgcg cgcgcggtcg atctgcgggt cctgcgggaa gtagccgtcg gcggcccgga
     1621 ccccggcggt gttcggcgcg accgcctcgt tgccgtcgga gtgggcggtc agcccggggg
     1681 cggcgtagac gccgatgtgc gagtgggtgt cgatgatgcc gggggtcagg gtcttgccgg
     1741 tcaggtcgac gacggtcgcg ccctcgggca cggcgaggtt ctccccgacc gcgacgatct
     1801 tgctgtcctc gaccagcagg tcggcgcgcg cgtagatctt gccggtcgcg gtcatgacgg
     1861 cggcgccgcg cagcagggtc ttgtggcccg ggccgcgcgg ctccgacttc gccgaagctg
     1921 gcgccttcgc caccgccggc tccggcggcc gggtctcgcc ctgcttgccg caggcgaggg
     1981 cggcgctcac agcaacgagc agcaggaccg gacggcgctt gatcacgccc gggaagctac
     2041 gccagcttgt tgcgccccgg aagtttttac aagttgtcgc cggtggcggg gcggggcccg
     2101 gggatatatc cttcgcgccc gcatgactcg ctccctcgcc cgttccccgg cccggctcat
     2161 tggctttagc gccgccttcc tcctcaccgt cgccaccggc gcgtgcctgc ccgagcgtgg
     2221 tggggtcccg gacaaggcgg ccgtcgccgc ggccggcaac aacggcccgg ccgccaaggc
     2281 cgcgggcagg gtcgcacagc cgaccccgcg cccgcagccg gaggtcgccc cggcc
//
LOCUS       DASWNJ010000268         7815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_464571, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000268 DASWNJ010000000
VERSION     DASWNJ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7815
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464571"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VGB85_04835"
     CDS             complement(<1..730)
                     /locus_tag="VGB85_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133377.1"
                     /translation="MPHQEDTSTQDHTLLTETETLCTRLHCLPAWAAKLVRLRDTVPT
                     GADSVIGTGLGGMYWDDKKKKPGQQTNKLELKEWSLRDIYNFIRSESKICNKYDSPGI
                     YAACPAVPADCHKTNFHHSRLIAIDLDGTIKDPDGLRRIQRLCGLWGCILHSTARHDP
                     RNGIYRLRILLLVTQAMTYEEHKRVAASVCSQIAAHLGASEGIDGCSERAFQAMLIPG
                     WADESFRDQAICELYEGDIMDNTLP"
     gene            complement(1049..1225)
                     /locus_tag="VGB85_04840"
     CDS             complement(1049..1225)
                     /locus_tag="VGB85_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133378.1"
                     /translation="MKNYNPTLPITIENWPAGLKMPPLRHSQQQAEPKAMKVPKIAAD
                     KAAEEKTSSFSFFA"
     gene            complement(1222..1479)
                     /locus_tag="VGB85_04845"
     CDS             complement(1222..1479)
                     /locus_tag="VGB85_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133379.1"
                     /translation="MSKTKFTLKVIKHPHRKMWVICGTAIPYIRDLFDELDSISCRIG
                     SWSTRSSRTAHHLDICAPALVALAHQRAAAAAAAAAKGVRR"
     gene            complement(1505..1642)
                     /locus_tag="VGB85_04850"
     CDS             complement(1505..1642)
                     /locus_tag="VGB85_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133380.1"
                     /translation="MITLDIVVHLLGLISLLGIVVAYERRIRSTTSTSTSTPADPRVG
                     K"
     gene            complement(1639..1905)
                     /locus_tag="VGB85_04855"
     CDS             complement(1639..1905)
                     /locus_tag="VGB85_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0133381.1"
                     /translation="MPNQNQIPAIMNITEASAAVKLHHTTLRKAIKEGRLPCSRPDGR
                     AIRITRKQLLTWLSGPKDEPERDPPDLLLECIRALAAGQKGVAR"
     gene            complement(2096..2560)
                     /locus_tag="VGB85_04860"
     CDS             complement(2096..2560)
                     /locus_tag="VGB85_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133382.1"
                     /translation="MLARWAVRLAVRQEAQLLLDEGADPDHLRDLRISCTTSLHGDKV
                     AMERWGASSLKACHDAQKRVRETQLVDGALYLSVLYDTHRDAPAPIYLGCGSGLDSGV
                     PGAGLAFPCSSQVDPTIRVKRYPEPEQTVLWDEDLTPWVYWSQVREEDEEKK"
     gene            3042..4241
                     /locus_tag="VGB85_04865"
     CDS             3042..4241
                     /locus_tag="VGB85_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY0133383.1"
                     /translation="MKISIRPHKTKQDRLLADMIPPPPYKRIRLLVPLGLDEAQAMAW
                     AQKEARTNLRVQREQVQEAEEEKIETDPNRTPTLAEFWEGRFAAEYLPSLRPGTRADT
                     GNCWALHIRPVLADQRIDQIDLKKIHELRDSMKGGALKKASSRNKVLQKLIATLRYAA
                     DCGIISRDAVPSRVRMEQLPKTSKAVFGAEDLDRMITAGKMKSIEHEVFALLLVHGCL
                     RIGELNALHWSDIDFQAKTMKVQRTEWMGTIQDAPKGHVGTVPLSSKLYDALVRLHAW
                     GGLRSPLVLPAKKREEWGVRHGPGLLISIQRLAAIRGSGPHLIRHSALTLAASRGASP
                     YALQALARHSNMSTTMRYYVHLQQVKVAQEAVSLLEGTPAPSTDGQLPANEVTAAPKV
                     RLVKSKR"
     gene            complement(4409..4723)
                     /locus_tag="VGB85_04870"
     CDS             complement(4409..4723)
                     /locus_tag="VGB85_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133384.1"
                     /translation="MKPETEQRWRVHVAAWANSGLTCKAYAAKVGINSRTLTWWKSKL
                     GEGVAPAAFVEVTSQVAAIAAPELGVVELIIHRVMVRLRGRVDVDALMRVLDVLEARS
                     QR"
     gene            complement(5258..7303)
                     /locus_tag="VGB85_04875"
     CDS             complement(5258..7303)
                     /locus_tag="VGB85_04875"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0133385.1"
                     /translation="MRRILGVLCFTCVTACGDDMTDSLTGGMTGSAATTLDPTTTLPT
                     SGVDGSGEETAADGSADGTADTGTNTEPPTTGPEPTSTTGTNGMTGTTDTTASTGTTS
                     TTGTTGTTGAPAMCDPMPAFDPEIVPIDESCKVDLQEGTFNPVIEWKYGSTSFAGPAA
                     VGQIVDTDASGALDDLDMPIILIYESSNVVALRGDGSGVAWQANGAYGGPGNGIALGD
                     LEGDGWPEVITGGFSNLCALDGQTGVQKWCVGLAPPDIGSNGYNFPAIADMDADGLAE
                     VTLGRVIVDSTGVVIAKGTLGKGGTPIYGAMSVPVDLDGDGQMELVTGNAAYDVDGNV
                     VWQNGASDGLVAVADFDLDGEGEIVSTIGTGVLGMETDGTVVWGPLPYPGNLGAPAID
                     DLDGDGTPELLFAAQNSLVAMKWGGAVFWTAPISDNSGAAGPSLFDFEKDGYPEVLYA
                     DEVAIRFFSGLDGSVKFFSDQHKSGTILEYPVVADVDNDDEVEIVLGHHTATRQIGAV
                     TVYGDADKTWPPGRKVWNQHAYSITNVGNLGEIPAVPQQNWPQYNSFRSADIGLPPGE
                     FIELLAELLDACTDGCDGAAYIAARVRNAGNVEAPAGIPVTLRAGPGGPVLATLMTTA
                     PIAPGATGEVLVFAVPASDLMGLAPEITVDDIGDGSGVIPECHEDNNVALGEPLVCR"
     gene            complement(7561..7734)
                     /locus_tag="VGB85_04880"
     CDS             complement(7561..7734)
                     /locus_tag="VGB85_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133386.1"
                     /translation="MGRTELGREGIDGGTFKVGTRGGLRVRWGETFLVRFDGAYSPTE
                     GTPRFYVDIGHVF"
BASE COUNT         1326 a         2508 c         2629 g         1352 t
ORIGIN      
        1 ggggcagggt gttatccatg atgtccccct catacagctc gcagatggcc tggtcacgga
       61 aggactcgtc agcccagccg gggatcaaca tggcctgaaa ggccctctcg gagcacccgt
      121 cgatgccctc cgacgcgccg aggtgggcgg cgatctgcga gcacacggag gcagccaccc
      181 gcttgtgctc ttcgtaggtc attgcctgcg tgacgagcaa caggatccgg agccgataga
      241 tcccgttgcg ggggtcatgg cgggccgtcg agtgcaaaat acagccccac agcccacaca
      301 agcgctggat ccgtcggaga ccatcagggt ctttaatggt cccgtcgagg tcgatggcga
      361 tcagccgaga gtggtggaaa tttgtcttgt ggcagtcggc cgggaccgca gggcaagctg
      421 catagatgcc cggcgagtcg tacttgttgc agatcttcga ctcgctgcgg atgaagttgt
      481 agatgtcgcg cagcgaccac tccttcaatt cgagcttgtt ggtctgctgc cctggcttct
      541 tctttttgtc atcccagtac atcccaccga ggccagtgcc gatcacactg tcggcgcccg
      601 tggggacggt gtcgcggagc cggaccaact tggccgccca tgcgggcaga cagtgaaggc
      661 gcgtgcagag ggtctcggtc tcggtgagga gcgtatggtc ttgtgtagat gtatcttctt
      721 ggtggggcat gagccgccct gctttccagc cccacacctg ccagggtgtt ggggcttctt
      781 tcgttggttg tctcgaagac cgcgacatgc ggtcctgctt ggattcgggc tattgggtca
      841 gcggcggacc tgcgggtagt tgcccgtggt atcctccggg agggtggcac tacatatagt
      901 ggccggtctc cccgggggca ccatatgcag tggtcggtcg gcccggcgga gaggcccgct
      961 atggccggag atcaaaactc acactcttga ttcgtatgcg aagaagatat ccgctccccc
     1021 tcgctatcgg gggcccggac gaaaagggtc aggcgaagaa cgagaagctc gaggtcttct
     1081 cctcggctgc cttgtcggca gcgatcttcg gaaccttcat ggccttgggt tcggcctgct
     1141 gctgcgagtg acggagcggc ggcatcttca aaccggcggg ccagttctcg atcgtgatag
     1201 gcaaggtggg gttgtagttc ttcaccggcg cacccccttc gcagcagcag cagcagcagc
     1261 agcagcacgc tgatgggcaa gggcaactag agcgggcgcg cagatgtcga ggtggtgtgc
     1321 cgtccgcgac gagcgtgtgg accaactgcc aatacggcaa gagatcgagt cgagttcgtc
     1381 gaacagatcc cggatgtaag ggatggccgt cccgcagatg acccacatct tgcggtgggg
     1441 gtgcttgatg accttcaagg tgaacttggt cttggacatg gtcttgatct cctggagttc
     1501 ggaactactt gccgacgcgc gggtcggcgg gggtggatgt ggatgtggat gtggtggacc
     1561 ggatgcgccg ctcgtatgcg acgacgatcc cgagcaggga gatgaggccg agcaagtgga
     1621 cgacgatgtc gagggtgatc atcgcgccac ccccttctga cccgcggcga gggcacggat
     1681 gcactcgagg aggaggtccg gcgggtcgcg ctcgggctcg tctttgggcc cagagagcca
     1741 cgtcaggagc tgcttccggg tgatcctgat ggcgcgtcca tcggggcgtg agcacggcag
     1801 gcggccctcc ttgatggcct tgcgcagggt ggtgtggtgc agcttcaccg cggcgctggc
     1861 ctcggtgatg ttcatgatgg cggggatctg gttctggttc ggcatgtcgg actccatacg
     1921 cttggcctgt cgggggcctg ttctggcctt gccctcgcct cgcgttctct tgtaggggtc
     1981 ttaatgccaa gctattctgg acctcgtaga tcgatagcag caccctgcca gctatcggcc
     2041 caggccagat ccgcttcttc ccgtaccgga gggaagaact taccgcttca aggaactact
     2101 tcttttcttc gtcctcttct cgaacctgcg accagtacac ccacggagtc aggtcttcgt
     2161 cccacaggac cgtctgctcg ggttcgggat accgcttcac cctgatagtc gggtcgacct
     2221 gcgacgagca cgggaacgcc aacccagcac cagggacacc cgagtcgagc ccactgccgc
     2281 agcccaagta gatcggcgcc ggcgcatcac ggtgggtgtc atacaagacg ctgaggtaga
     2341 gggcgccatc tacaagctgg gtttcccgga cgcgcttctg ggcgtcgtgg caggctttca
     2401 agctcgaagc accccacctc tccatcgcca ccttgtcgcc gtgaagggaa gtagtgcagg
     2461 agatgcgaag gtcccgtagg tgatctgggt cggcgccctc gtcgagtagg agctgggctt
     2521 cttgtcggac tgcgagccgg acagcccagc gagccagcat gcgagcttga acctgctcgc
     2581 tctcggcgag gggaccttcg ggagcgggtg gatagaggag cgcttccggg gcatccccag
     2641 gcgctacccg aagataggaa gagggttatc taactacttc tcggatcgtc agattgacct
     2701 caactgcgcc cgcacgggct tctgcaccta tgaactaaca acctcgtggg gggatcggaa
     2761 ataagaagcc taagccggtg atgccaaaca agcgcgcatc acgcaggtcg ggaccttccc
     2821 aagaaatcga caacggggcc cagggcccga agtacatgac cccggaggaa gcagccttcg
     2881 agcttcgcat ctccccgaaa acaatgagca actggagaag ccagggccac ggacccacct
     2941 ggacgaaaag cgggggcagg gttgcgtacc gacccgaagc tgtgcgggag tggtcactgc
     3001 aacaggaacg cggggcggag gccaagaagg caacttccga gatgaagatc agcatccgcc
     3061 cccacaagac aaaacaagac cgcctcctgg cagacatgat ccctccgccc ccatacaagc
     3121 gcatccgcct cctcgtcccc ctcgggctcg atgaagcgca ggcgatggca tgggcacaga
     3181 aggaagcccg cacgaacctc cgggtccagc gggaacaagt ccaggaagca gaagaggaga
     3241 agatcgagac cgacccaaac cgaaccccca cgctcgccga gttctgggaa gggaggttcg
     3301 cggcggagta cctgccgagc ctccgacctg gaacccgagc cgacaccggc aactgctggg
     3361 cgcttcacat ccgcccggtc ctcgccgacc agcgcatcga ccagatcgat ctcaagaaga
     3421 tccacgagct gcgcgacagc atgaagggtg gcgccttgaa gaaggccagc tcccgcaaca
     3481 aggtgctcca aaaactgatc gccaccctgc gctatgctgc cgactgcggc atcatcagcc
     3541 gggatgccgt gccctcgagg gtgcggatgg aacaactgcc gaagacctcg aaagcggtct
     3601 ttggcgccga agacctggac cggatgatca cggccgggaa gatgaagagc atcgagcacg
     3661 aggtcttcgc cctcttgctc gtccatggct gcctccgcat cggagaactc aacgcactgc
     3721 actggtccga catcgacttc caggcgaaga ccatgaaggt ccagcggacc gagtggatgg
     3781 ggacgatcca ggatgccccc aagggacacg ttggcaccgt gcccctgtcg tcgaagctct
     3841 atgacgccct ggtgaggctt catgcctggg gagggctccg tagtcctctg gtcctgcctg
     3901 ccaagaagcg ggaagagtgg ggggtccggc atggtccggg cttgctcatc tcgatccagc
     3961 gactggcggc gatccgtggg agcggacctc acctgatccg gcactccgcg ctgaccctgg
     4021 cggcatccag gggggcgagc ccctacgccc tccaggcgtt ggcccggcac tcgaacatga
     4081 gcacgacgat gcgctattac gtccaccttc agcaggtgaa ggtcgcgcag gaagccgtgt
     4141 cgctcttgga gggcactcct gctccgagca cggacggtca actgcctgca aacgaagtca
     4201 cggccgcacc aaaggtgcga ctggtgaagt ccaagcgatg attcgcactg gatgaagtac
     4261 tgacgccctg actcctcgga tcggcccaaa tcccgctctc cgccccctct cctgcatttc
     4321 tgggccgatc cgagaagtcc cgtcaccgcg tcgtcgtcgc gcacggggcc gtgctcgagt
     4381 ctggcggcca ggcggcgctg cgcgacggct atcgttggct gcgggcctcc agtacgtcga
     4441 ggacccgcat gagcgcgtca acatcgaccc gaccacggag tcgcaccatg acacggtgga
     4501 tgattagctc gacgacgccg agctcgggcg cggcaatcgc cgcgacctgc gaggtcacct
     4561 cgacgaacgc cgccggcgcg accccttcgc ccagcttcga cttccaccac gtcagcgtcc
     4621 gcgagttaat gccgaccttc gccgcgtacg ctttgcacgt caggccgctg ttcgcccagg
     4681 cggcgacgtg gacccgccag cgctgctccg tctcgggctt cataggcccc acttcctgcg
     4741 ccacacctcg actcgctcac gcagcgtccg gcgccgtcag gcctatgccg tcacgacgcg
     4801 gtagaccgaa cgcttacgat taaacaacgt cgcgtagacc ggagatcaaa ggaagcgcgt
     4861 gacatgacct gcgatggtgg tgggttgggc gttgaatcgt tcgaaggtcg cggcctgcgg
     4921 tggtccggaa gaagccgtcg tgggtagtgg tccgcttggt tgtcttccag acgccctcga
     4981 tggggttgag ttcggggaag tgaggcggca ggcgcaagag gttgatgcgg tgacggttgc
     5041 ggcgagccac gcaaagcgcg gccctcaacg gccctggtac tggggcgggc gagcgccgtc
     5101 gcacgagggg tccggttgtg agccgcgatg cgctgacccg aacagcgtga tggcgcgaag
     5161 ggggcgcttg ccgcccagaa ccgtgagcct gagcacggca ccgcgtcgga ccgagctgag
     5221 ggcgtgcggg gccgagcgcg atacacggag cgcgcggtca gcggcacacg agcggctcgc
     5281 cgagggcgac gttattgtcc tcatggcact cggggatgac gccgctgccg tccccgatgt
     5341 cgtcgaccgt gatctcgggc gcgaggccca tcaggtcgct cgccggcacc gcgaacacca
     5401 ggacctcgcc ggtcgcgccc ggtgcgatcg gggcggtcgt catcagggtc gccaggaccg
     5461 ggccgcccgg gccggcgcgc agggtcaccg ggattcccgc gggcgcctcg acgttgccgg
     5521 cgttgcgcac acgcgcggcg atatacgccg cgccgtcgca gccgtcggtg cacgcgtcga
     5581 gcagctcggc gagcagctcg atgaactcgc ccggcggcag gccgatgtcg gccgagcgga
     5641 agctgttgta ctgcggccag ttctgctgcg gcaccgccgg gatctcgccg aggttgccga
     5701 cgttggtgat gctgtatgca tgctggttcc agaccttgcg ccccggcggc cacgtcttgt
     5761 cggcgtcgcc gtagacggtc acggcgccga tctggcgggt ggcggtgtgg tggccaagga
     5821 cgatctcgac ctcgtcgtca ttatcgacgt cggcgacgac tgggtactcg aggatcgtgc
     5881 cgctcttgtg ctggtcggag aagaacttga ccgagccgtc gagccccgag aaaaagcgga
     5941 tcgccacctc gtcggcgtag aggacctcgg ggtaaccgtc cttttcgaag tcgaacaggc
     6001 tcggaccggc ggcgccgctg ttgtcggaga tcggcgcggt ccagaacacc gcgccgcccc
     6061 acttcatcgc caccagcgag ttctgggccg cgaacagcag ctcgggggtg ccgtcgccgt
     6121 cgaggtcgtc gatcgccggc gcgccgaggt tgccagggta cgggagcggg ccccagacca
     6181 ccgtcccgtc ggtctccatg ccgagcaccc ccgtgccgat ggtgctgacg atctctccct
     6241 cgccgtcgag gtcgaagtcg gcgaccgcga cgaggccgtc gctggcgccg ttttgccaga
     6301 ccacgttgcc gtcgacgtcg taggccgcgt tgcccgtgac aagctccatc tgcccgtcgc
     6361 cgtcgaggtc gaccggcacc gacatcgcgc catagatcgg ggtgccgccc ttgccgagcg
     6421 tgcccttggc gatgacgacg ccggtcgagt cgacgatgac gcgcccgagc gtgacctcgg
     6481 cgagcccgtc cgcgtccatg tcggcgatcg cggggaagtt gtagccgttg ctaccgatgt
     6541 ccggcggcgc gagcccgacg caccacttct ggacgcccgt ctggccgtcc agggcgcaga
     6601 ggttgctaaa cccgccggtg atcacctcgg gccagccgtc gccctcgagg tccccgaggg
     6661 cgatcccgtt gccggggccg ccgtacgcgc cattggcctg ccacgccacc ccgctgccgt
     6721 cgccgcgtaa ggctacgacg ttggaggact catagatcag gatgatcggc atgtccaggt
     6781 cgtcgagcgc gccgctggcg tcggtgtcga cgatctgtcc gaccgccgct ggcccggcga
     6841 agctcgtcga gccgtacttc cactcgatca ccgggttgaa cgtgccctcc tgcaggtcga
     6901 ccttgcacga ctcgtcgatc ggcacgatct cggggtcgaa cgcgggcatc gggtcgcaca
     6961 tcgccggcgc gccggtcgtg ccggtcgtgc cggtcgtgct ggtcgtgccg gtcgacgcgg
     7021 tcgtgtcggt cgtgcccgtc atgccgttcg tgccggtcgt gctggtgggc tcgggtccgg
     7081 tcgtcggcgg ctcggtgttt gtcccggtgt cggcggtgcc gtcggcgctg ccgtcggcgg
     7141 ccgtctcctc gccgctgccg tcgaccccgc tcgtcggcag cgtcgtcgtc ggatcgaggg
     7201 tcgtcgcggc ggagccggtc atcccgcccg tcagcgagtc ggtcatgtcg tcgccgcaag
     7261 cggtcacgca ggtgaaacaa agaacaccaa ggattctacg catactactc gctcatacca
     7321 ctcgcgggcc ctggcgtata cgtcatctcg ctccgcggtc gatcgcggcc gcgtccgcgg
     7381 ggccggatcg cgctcactcc gcggccgcgc cgccctcgct atcgagcagc gcgccgcaca
     7441 tccgcgcgtg tggcgggccg agcagccgtg gcgcgcgttc tccgggacgg cacccgggag
     7501 acccgcgatc tctgcagaga cgggcgcgat gagtacgtgc atagtggcgc ggtttcgcgg
     7561 tcaaaagacg tggccgatat cgacgtagaa gcggggcgtg ccctcggtcg gcgagtaggc
     7621 gccgtcgaag cggaccaaga acgtctcgcc ccagcgcacg cgcagaccgc cgcgggtacc
     7681 gaccttgaag gtgccgccgt cgataccctc gcggccgagc tcggttcggc ccaagtcgcg
     7741 ctcaagaggt tccagtcggg aattcctcat ctgcccgggg tactggggcg ggcgagcgcc
     7801 gtcgcacgag gggtc
//
LOCUS       DASWNJ010000269         1904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_499065, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000269 DASWNJ010000000
VERSION     DASWNJ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499065"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1156
                     /locus_tag="VGB85_04885"
     CDS             77..1156
                     /locus_tag="VGB85_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HEY0133387.1"
                     /translation="MTPIRLGINGMGRIGRLALRHALARATADATGSGAARKGPPVVV
                     VAANDLAEADELAYLLTHDSVHRWNLPKVKAEGDSLRVNDGKIALFKEKDPSKIPWNA
                     HGVDIVIEATGRFTDRAGMAAHRTASDGPSHVVLAAPGKGVDKTIVVGVNEGTLDPEK
                     DRLISNASCTTNALTPVLATLDKVFGLRWGLVGTTHAYTNGQGIIDTLSGKDLRRGRA
                     GAINIVPTSTGAAKAISEVIPNLRGKVDGTAVRVPVVDGSLYDLVCTLEGSPSLERVI
                     EALREAAATEALQGILDVRKEALVSSDILGDPHSSVVDVDACMGLGDLVKIVGWYDNE
                     WGYTTRLLDLVAIVGSWISSQQRAV"
     gene            1350..>1904
                     /locus_tag="VGB85_04890"
     CDS             1350..>1904
                     /locus_tag="VGB85_04890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0133388.1"
                     /translation="MHTPELDPRDVVAAAQLGVWDFYPQTGTLVWSPRCREIFGLTPE
                     ASVDYGVFLRAVHIDDRARVSQAVQKALTAGGDPHIDLEYRGVRAADGAQRWLWALGE
                     ASFDAAGVPSRLVGVVVDITERKQRALQDRNLTLVADNSADFIAFASPEGKLLYLNPA
                     GLALVGLDDLAEVQGHEMIDLFAPE"
BASE COUNT          285 a          676 c          677 g          266 t
ORIGIN      
        1 cggcgatgaa tagggcgggt ggggtgtggg tgcggcgttg ccggacccgg gtgcttgacc
       61 tatcctccgc gacgctatga cgccaattcg cctcgggatc aacggaatgg gccgcatcgg
      121 ccgcctcgca cttcgtcacg cgctcgcgcg ggccacggcc gacgcgacgg gatccggcgc
      181 ggctcgcaag ggtccgccgg tggtggtggt ggccgcgaac gacctcgccg aggccgatga
      241 gctggcctac ctgctgacgc acgactcggt gcaccgctgg aacctgccca aggtcaaggc
      301 cgagggcgac tcgctgcgcg tcaacgacgg caagatcgcg ctgttcaagg agaaggaccc
      361 gagcaagatc ccgtggaacg cccacggcgt cgacatcgtc atcgaggcga ccggccgctt
      421 caccgaccgc gcgggcatgg cggcgcaccg caccgccagc gacggcccga gccacgtcgt
      481 gctcgccgcg ccgggcaagg gcgtcgacaa gacgatcgtc gtcggcgtca acgagggcac
      541 cctcgacccc gagaaggacc gcctgatctc caacgcgagc tgcacgacca acgcgctgac
      601 gccggtgctg gcgaccctcg acaaggtctt cgggctgcgc tggggcctgg tcggcacgac
      661 ccacgcttac accaacggcc agggcatcat cgacacgctg tccggcaagg acctccgccg
      721 cggccgcgcc ggcgcgatca acatcgtccc gacctcgacc ggcgccgcca aggcgatctc
      781 cgaggtcatc ccgaacctgc gcggcaaggt cgacggcacc gccgtgcgcg tgccggtggt
      841 cgacggctcg ctgtacgacc tcgtctgcac gctcgagggc agcccgagcc tcgagcgcgt
      901 gatcgaggcg ctgcgcgagg cggcggccac cgaggcgctg caggggatcc tcgacgtccg
      961 caaggaggcg ctggtgtcct cggacatcct cggcgacccg cactcgagcg tcgtcgacgt
     1021 cgacgcctgc atgggcctcg gcgacctggt gaagatcgtc ggctggtacg acaacgagtg
     1081 gggctacacg acccgcctgc tcgacctcgt ggcgatcgtc ggcagctgga tctcgtcgca
     1141 gcagcgcgcg gtctgaggcg accgcgcatc gcggcggtca cgcgagcggc gggggcatcc
     1201 gccgcggctc gcggtccgtc gctcacaccg ctgttttacg gcccgggaca tgcgtgaacc
     1261 cgggttccat ggcatcgacg ctgcgatgtc cccgtgcact gcgaaccgcg cggcctcgtc
     1321 gggcgctggc gcccccgaag gcgatcttta tgcacacgcc tgagctcgac ccgcgtgacg
     1381 tcgtcgccgc cgcgcagctc ggcgtgtggg acttttatcc gcagaccggc acgctggtgt
     1441 ggtcgccgcg ctgccgggag atcttcgggc tgacgcccga ggcgagcgtc gactacgggg
     1501 tgtttctgcg ggccgtgcac atcgacgacc gcgcccgcgt cagccaggcg gtacaaaagg
     1561 cgctgacggc cgggggtgac ccgcacatcg acctcgaata ccggggcgtg cgcgcggccg
     1621 acggcgcgca gcgctggctg tgggcgctcg gcgaggcgtc gttcgacgcg gccggggtgc
     1681 cgagccgcct cgtcggcgtg gtcgtcgaca tcacggaacg caagcagcgc gcgctgcagg
     1741 accgcaacct gacgctcgtc gcggacaaca gcgcggactt tatcgccttc gcgtcgcccg
     1801 agggcaagct gctgtatctc aacccggccg ggctcgcgct cgtcggcctc gacgacctcg
     1861 cggaggtgca gggccacgag atgatcgact tgttcgcgcc cgag
//
LOCUS       DASWNJ010000270         4759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_499099, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000270 DASWNJ010000000
VERSION     DASWNJ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499099"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VGB85_04895"
     CDS             <1..487
                     /locus_tag="VGB85_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133389.1"
                     /translation="DDEHAAQALSALRDHAAAPAMRALLARSQGATCVRVALALHFLR
                     RQPALARHLISVLHGPEEGAPWHGSGRLDAATGLRHFRGRADEAALFAAVGDPAYLIR
                     HHACNSLLSRFGVARPDVDRYPKIFALLRGPDEAPPDAQAFAGYEAARELLRGIKARA
                     R"
     gene            complement(636..2456)
                     /locus_tag="VGB85_04900"
     CDS             complement(636..2456)
                     /locus_tag="VGB85_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133390.1"
                     /translation="MRSLSVALALLLASPVAYAQEPERVIMKLDEFLKLYEKNRAEET
                     QAPIDFAISSARYSGEVQLRDGEPYAAVFTAKLRIDVLRGKGYVKVPVLPATVALESA
                     KIGAAEADVIIENGYYTLVTAQRGALTLDLNFAVAVTSAEGASGLNFQLVPAGATTAV
                     LSVPARDDLDFTVTNAKLQSDKVVGDKRVVEATLPSTGSLAIQWQREIPKAAQQPSRV
                     FAEVHTLVGIGDGLMRATTTVQYTILFAGVERLQLKVPRGMQMLDVIGNGIRDWKLDP
                     EGTLTVTLNYAARGVYPLRFEMERVIGKTEALAAPIAAPVGVDRVKGFVGVESRGNLE
                     IKSGEVSNATPVDVRALPAAILGITSQPVLLGYKYLGEDAKIPLTAAQHEDVDVLVTL
                     LDETRARTMWTPEGRRLTSVKYRIRNNRRQFLRLALPKGAELWSASVGGRAVQPARAS
                     DARVMIPLVRSQATGGELAAFEVEIVYVEAGVAPSSRGRGSFSAELPRPDAPSTYVAW
                     TVYAPLDAKVRRFTVEGTLRRVKQLSNPIPAEDMNYVQTANPEVAQQAVTQANTGSLG
                     DGAVPVPVSLPLQGREVNFERLLALDESLAIRFKYRGLRG"
     gene            complement(2486..>4759)
                     /locus_tag="VGB85_04905"
     CDS             complement(2486..>4759)
                     /locus_tag="VGB85_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133391.1"
                     /translation="ASGARGSTDAPHSFIGAPAIVDLSFGTPPAAPGSDAAVVVARAG
                     LGLTVGDAELRGRGRLVWELRRGSVTSVGFTATGVGADLEVTGPGVREVQRSGDSVRV
                     LLQSPVSGRLELALRWTSPIGKATESRVDLPQIRFAEVFRAESSLQLARDGEVEAVPA
                     VERWTPSSAAGLPAWGQGLVEGAPTAAFTAATEASGHLELLRFVPIEGPPVVVDVAAY
                     TIATSREGRALMRAHYEVRNERAATLRIRPPPGMEIVGVRVAGATAVIARDRDGSWRV
                     PLPRSVDTVSGLLSFPVEVGLLGEVVHKGSGSEPVPWARRERRSLPLPMLDAPVAVLR
                     VTLHLPPGYRSRLRPGDGEVVAAFTRGEGITYGLGVGAVGAAEADALFQSAVKRWLDN
                     DFGGAQAELDRLRQLGARNENIDRLQGNLDLIAGRGKQDATVSRRIREQAKARAVSDQ
                     VAQQELERKAEESRRSGDYKSAESQYRAALAVGDRLAKLEQSESVEQSTRNEQLRGEL
                     ASNAKEARQSEVVQVSGKSRRAGLRFKSSARDDPAGSASSASVAGDGKKLADKDGEAS
                     KTPTGGEDVDGAVDAGNVYGGMIGEPIAEESAPVEPAAPPPPVTTPVSGTSRDFTAVV
                     SAVPGVTHDGAVDISRSAAESSYTLDGASTSGFIKRARRSIAARREGRQARAAAVKDV
                     RGRGSIKTRDTLDTKFKPTDDEAGSGLAAPVVTASALSVVVPAVGVAVLYERALLPAG
                     AEHRVDISAREPLISRD"
BASE COUNT          644 a         1811 c         1627 g          677 t
ORIGIN      
        1 cgacgacgag cacgcggccc aggccctgtc cgcgttgcgt gaccacgcgg cggcgccggc
       61 gatgcgggcc ctgctcgcgc gctcgcaggg cgcgacgtgc gtacgcgtgg cgctcgccct
      121 gcacttcctc cggcgccagc ctgccctcgc caggcacctg atctccgtgc tgcacggccc
      181 cgaggagggc gccccgtggc acggctccgg gcggctcgac gcggcgaccg gcctacgcca
      241 ctttcgcggc cgcgccgacg aggccgcgtt gttcgccgct gtcggtgacc cggcctatct
      301 cattcgccac cacgcctgca attcattgct ctccaggttc ggcgtcgccc gccccgacgt
      361 cgatcgctac ccgaagatct tcgcgctgct ccgcggcccc gacgaggccc cacccgacgc
      421 gcaggcgttc gccggatacg aggccgcccg cgagctcctg cgtgggatca aggcccgcgc
      481 ccgctgacgt gcgcccgcca tgcatttaag aaggcccgct cgcgcccgcg gtccagcgta
      541 tcgagggcga tcgcctgcgc gccccaccag cgcagcgtgt cgtcgagcgt ggtgtcgagg
      601 tcgcgaaacc gcaggcgaaa agtctacaca tatcgtcacc cgcgcaggcc gcgatatttg
      661 aagcggatcg ccagcgactc gtcgagcgcg agcaggcgct cgaagttcac ctcgcggcct
      721 tgcagcggca agctgaccgg caccggcacc gcgccgtcgc cgagcgagcc ggtgttggcc
      781 tgcgtcaccg cctgctgcgc gacctcgggg ttcgcggtct gcacatagtt catgtcctcc
      841 gcggggatcg ggttggacag ctgcttgacg cggcgcagcg tgccctcgac ggtgaacctg
      901 cgcaccttgg cgtcgagcgg ggcgtacacc gtccaggcga cgtaggtgct cggcgcgtcg
      961 gggcgcggca gctcggcgct gaagctgccg cgaccgcggg agctcggcgc gacgccggcc
     1021 tcgacgtaca cgatctcgac ctcgaaggcc gccagctcgc cgccggtcgc ctgcgagcgg
     1081 accagcggga tcatcacgcg cgcgtcgctg gcccgcgccg gctggaccgc gcggccgccg
     1141 accgaggcgc tccacagctc ggcgcccttt ggcagcgcga ggcgcaggaa ctggcgccgg
     1201 ttgttgcgga tcctgtactt caccgaggtc aggcgccggc cctcgggcgt ccacatcgtg
     1261 cgcgcgcggg tctcatccaa cagagtcacc agcacgtcga cgtcctcgtg ctgcgcggcg
     1321 gtcaagggga tcttcgcgtc ctcaccgaga tacttgtagc cgagcaatac cggctggctg
     1381 gtgatcccga ggatcgcggc aggcaacgcg cgcacgtcga ccggcgtcgc gttgctgacc
     1441 tcgccggact taatctcgag gttaccgcgc gactcgacgc cgacgaagcc cttgacgcgg
     1501 tcgaccccga ccggcgcggc gatcggcgcc gcgagggcct cggtcttgcc gatcacgcgc
     1561 tccatctcga agcgcagcgg gtacaccccg cgcgccgcgt agttgagcgt caccgtcaac
     1621 gtgccctcgg ggtcgagctt ccagtcgcgg atgccgttgc cgatcacgtc gagcatctgc
     1681 atgcccctcg ggaccttgag ctgcagccgc tcgacgcccg cgaacaggat cgtgtactgc
     1741 accgtcgtcg tcgcgcgcat caggccgtcg ccgatgccga ccaacgtgtg cacctcggcg
     1801 aacacgcgcg agggctgctg cgccgccttc gggatctcgc gctgccactg gatcgccagc
     1861 gagccggtcg agggcagcgt cgcctcgacg actcgcttgt cgccgacgac cttgtcggac
     1921 tgcagcttgg cgttggtgac cgtgaagtcg aggtcgtcgc gcgccggcac cgacaggacc
     1981 gcagtcgtcg cgccggccgg gaccagctgg aaattgagcc cgctcgcgcc ctcggccgag
     2041 gtgacggcga cggcgaaatt gaggtcgagc gtcagcgccc cgcgctgcgc ggtcaccagc
     2101 gtgtagtaac cattctcaat gatgacatcg gcctcggccg cgccgatctt ggccgactcg
     2161 agcgcgacgg tcgccggcaa caccggcacc ttgacatagc ccttgccgcg caacacgtcg
     2221 atccgcagct tcgcggtgaa caccgccgcg tagggctcgc cgtcacgcag ctggacctcg
     2281 ccgctgtagc gcgccgacga gatcgcaaag tcgatcggcg cctgcgtctc ctcggcgcgg
     2341 ttcttctcgt agagcttcaa aaattcgtcg agcttcatga tcacgcgctc gggctcctgc
     2401 gcgtacgcga cggggctggc gagcaggagg gcgagggcta cggagaggct gcgcatggct
     2461 gtccttgggg gcatttcgag aggggtcaat cgcgggagat caggggttcg cgggccgaga
     2521 tgtcgacccg gtgttctgcg ccggccggca acagcgcgcg ctcgtagagc accgcgacgc
     2581 cgaccgcagg cacgaccacc gagagcgccg atgcagtgac caccggcgcc gccaggcccg
     2641 acccggcctc gtcatcggtg ggcttgaact tggtatcgag ggtgtccctg gtcttgatcg
     2701 acccgcgtcc gcgaacatcc ttgacggcgg ccgcccgcgc ctgccggccc tcgcgtcgcg
     2761 cggcgatcga ccggcgcgcg cgtttaataa agcccgaggt gctcgcgccg tcgagcgtgt
     2821 agctcgactc ggcggccgat cgcgagatat cgacagcacc atcatgggtc accccgggca
     2881 cggccgacac gaccgccgtg aagtcgcggc tcgtgccgct gaccggcgtg gtcacgggag
     2941 gcggcggggc ggcgggctcg accggcgcgg actcctcggc gatcggctcg ccgatcatgc
     3001 caccatatac attcccggca tcgacggcgc cgtcgacgtc ctcaccgcct gtcggggtct
     3061 tgctcgcctc gccatccttg tccgcgagct tcttgccgtc gcccgcgacg ctggccgagg
     3121 acgccgaccc ggccggatcg tcgcgggcgc tggacttgaa gcgcagcccg gcccggcgcg
     3181 acttgccgga gacctggacg acctcgctct gcctggcctc cttggcgttc gacgcgagct
     3241 caccgcgcag ctgctcgttg cgcgtcgact gctcgaccga ctcgctctgc tcgagcttcg
     3301 ccaggcggtc gccgaccgcg agcgcggcgc ggtactggga ctccgccgac ttgtagtcac
     3361 ccgagcgccg gctctcctcc gccttgcgct cgagctcctg ctgcgcgacc tggtcgctga
     3421 ccgcccgcgc cttggcctgc tcgcggatcc gccgcgacac cgtcgcgtcc tgcttgccgc
     3481 gaccggcgat caggtcgagg ttgccctgca ggcggtcgat gttctcgttc ctcgcgccga
     3541 gctgccgcag cctgtcgagc tcggcctgcg cgccgccgaa gtcgttgtcg agccagcgct
     3601 tgaccgccga ctggaacagc gcgtcggcct cggccgcccc gaccgccccg acgccgagcc
     3661 cgtacgtgat gccctccccg cgcgtgaacg ctgcgaccac ctcgccgtcg cccggccgca
     3721 gccgcgagcg atagccgggc ggcaggtgca gggtcacgcg cagcacggcg accggggcgt
     3781 cgagcatcgg cagcggcagc gaccggcgct cgcgcctggc ccacgggaca ggttcggatc
     3841 ccgacccttt gtgcaccacc tcgccgagaa ggccgacctc gaccgggaac gacagcaggc
     3901 cggacaccgt gtcgaccgag cgcggcagcg gcacgcgcca cgagccgtcg cggtcgcggg
     3961 cgatcaccgc cgtcgctccg gcgacgcgca ccccgacgat ctccatgccc ggcggcggac
     4021 ggatccgcag ggtcgccgcg cgctcgttgc gaacctcgta gtgggcccgc atcagcgccc
     4081 gaccctcgcg gctggtggcg atcgtgtaag ccgcgacgtc gacgacaacc ggcgggcctt
     4141 cgatcggcac gaagcgcagc agctcgaggt gaccgctcgc ctccgtcgcc gccgtgaacg
     4201 cggccgtcgg cgcgccctcg accaggccct gcccccacgc cggcagcccg gcggcagagc
     4261 tcggcgtcca gcgctcgacc gcgggcaccg cctcgacctc gccgtcgcgc gcgagctgca
     4321 ggctcgactc ggcgcggaac acctcggcga agcggatctg cggcaggtcg acccgcgact
     4381 cggtggcctt gccgatcggg ctggtccagc gcagcgcgag ctcgaggcgc ccgctcaccg
     4441 gcgactgcaa cagcacgcgc acgctgtcgc cgctgcgctg gacctcgcgc acgcccggac
     4501 ccgtgacctc gaggtcggcg ccgacgcccg tcgccgtgaa gccgaccgaa gtgaccgacc
     4561 cgcggcgcag ctcccacacc agccgcccgc gcccgcgcag ctcggcgtcc ccgaccgtca
     4621 gcccgagccc cgcccgcgcg accaccaccg ccgcgtcgct ccccggcgcc gccggaggtg
     4681 tcccgaagga caggtcgacg atcgccggcg cgccgatgaa gctgtgcggc gcgtcggtcg
     4741 agccgcgcgc gccgctggc
//
LOCUS       DASWNJ010000271         6313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_429173, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000271 DASWNJ010000000
VERSION     DASWNJ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6313
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429173"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(631..831)
                     /locus_tag="VGB85_04910"
     CDS             complement(631..831)
                     /locus_tag="VGB85_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133392.1"
                     /translation="MDGKVLCVATTVDSIAAEQCVVVSCTHDGPLAGDVSVVGNDDGM
                     GGKNTLECIYTNNDVVAQISCQ"
     gene            complement(1343..1864)
                     /locus_tag="VGB85_04915"
     CDS             complement(1343..1864)
                     /locus_tag="VGB85_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133393.1"
                     /translation="MRGADPPPRGPHLPRPRHDLSWSFTAARTAGLVGCVRAELGDPL
                     GDIAIHNADYPTPEGPISVEVALAWHTHPYASEPQIHSFANLLRTDDGQHVDGLLDGI
                     RAFLPRRTGKRRLGGLVAAVSVILADVHYGSPTRRSLANSSARPAVKAATLAALEAWA
                     TRSPEAAASLRAR"
     gene            complement(1851..2384)
                     /locus_tag="VGB85_04920"
     CDS             complement(1851..2384)
                     /locus_tag="VGB85_04920"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0133394.1"
                     /translation="MPDEYTADGIESLTLRESVRRRPGMYAGDTATGDGALHLVLEVL
                     SNAVDQVMLGRCTRVDIRVDADDLVTISDDGPGIPAHGLVALLERPSNRPTVDGHRPH
                     VHLSPIGLGLPVSNALSDPFEVDTVHAGVHASVRYAGGLVQTPLQVRPATRPSGTAIR
                     LRPDPHVFAALASLAWS"
     gene            complement(2522..3385)
                     /locus_tag="VGB85_04925"
     CDS             complement(2522..3385)
                     /locus_tag="VGB85_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133395.1"
                     /translation="MPRLLADYAVPAGAGLVDLQVEMLELGAISVHGATVVELTQETF
                     QCFFRCLGDTVPPDVSEELRRQLNSDHPAELDGELLRAAGAPLPAGLYDVIDAEAPVT
                     GVRHTGICVNCPSDYRAEAGADAWGVEVREAGTQVRIGYGRDVPQPTATLLYRASLGE
                     LGLVLLPLTGAPDRVRIDHYAGRLASGARPTVLAFGSYANERCAVAVVLDGHHKLAAA
                     AAAGSPLGLLVFLSSRLLVSYADCRLFHGHPSVPTELARRRITGKTQALHAWADELEV
                     TRHHRPPRRGS"
     gene            complement(3435..4472)
                     /locus_tag="VGB85_04930"
     CDS             complement(3435..4472)
                     /locus_tag="VGB85_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133396.1"
                     /translation="MRRRLDRLFTFARAAENSPLENFTTEALACAIEQDPGPLLRTLP
                     REVADLTAFSEFRDLRVDTQVSVPGGIVDLVVEVIVDGAPLHLWIEVKAHAGLSGAQL
                     SAYAAAAAAWPSTPQPVVFMLCKYPLTRDVPTLRWNRLRACIPVDCHYTWLELRDFLE
                     DHRMADDMDAPFTATQLGAAGLARGLLRKATRLVHEFLGRPDLPVAWHESNFPRSEAK
                     ISRAVAVQLFHHNRLVVGSRRYPWVCFGIYFRDSPELTLWIEFQPGDHVAYDAIAALV
                     PGLPPGWTVEWNRVSPWIGASLAIQGDLEHEAAMTWLLARVHELAAANILAALPKWRR
                     HDRVGDDDETD"
     gene            complement(5196..>6313)
                     /locus_tag="VGB85_04935"
     CDS             complement(5196..>6313)
                     /locus_tag="VGB85_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133397.1"
                     /translation="YDGQESLHNAVKAALSLLAQWMADHTADAVPVGEALAILADTFA
                     NFPLEGRAWLEVLEGSDLLRRDTAPREGPVDDWSNPSEVVRFAFQRLQDNLIARSLLA
                     RCNDVERAFADGAMLSFLIQRIPERGKNVVHIHQQWAGVVSAIWSALAEVHGKELVDL
                     ASFFNDDGRLLKTHQFKRVFRESVRDRRQNAITQRTRQILNDLFDASPEEKIEVLLSA
                     ACVPSHPWNARALHERLRALSIEDRTNFWVRPLSAIRTSELRDTVSNLIDWTLHADLG
                     VASPEVVELAIISLAWLSVDSSIDGYHDVKRALEVVSHAYPSIVESTRTLFMAFDQDA
                     LNVPETGSNVDPSSGHELSGSRDDERVEQSGEREEQE"
BASE COUNT         1118 a         1917 c         2229 g         1049 t
ORIGIN      
        1 cgtcgaagcc tccgccgctg cgcaagatgc taactgggct ccagtgttca agagcgccag
       61 cgccgctgct gacgcagcca gtggagccgc atatggagcg gaggaggtcg tagctcgccg
      121 tcatgttgcg gtgcaggccg cgctgaaggg caagggtcga cggcagcgac ggattcgtaa
      181 ggtggcaagt aagagccttt cgcgcgccga acgcaccgcc cggaagatct cccgattcgc
      241 aaagccgtta ggccccctgg ggatggcagc tggtcccctg ggggaactgg cagagactag
      301 aagaaagcgt cgttcgaccg ctgaatccgc tgcacgcgcc ggaatatcta cggcggcctc
      361 cgccgctggg gcggccgcgg gctcgacggc cgcggttccg tgcgccgccg tctcattagg
      421 cgtcgccggg ccggtatgtg gtgcggttgg tgcggccgtg gggggcgccg ttgtggggaa
      481 aattggagaa aggtcggtga tttgttgttc ggcgatgact aaatagctgg cctggtcagg
      541 agccgcatcg ctcggtgtgt acgagtccaa gtagccgggg gccaatttac gaaccaaagc
      601 gtggctggag gccgcgctcc gagggatcgc ctactggcag ctgatctggg cgacgacgtc
      661 gttgttggta tagatgcact cgagggtgtt cttgccgccc ataccatcgt cgttgccgac
      721 gacgctgaca tcaccggcga gcggcccgtc atgggtgcag gagacgacga cgcactgctc
      781 ggcggcgatc gagtcgacgg tggtggcgac gcacaggacc ttgccgtcca ccggggcgcc
      841 ctcgtagaag gcggtcttga ggccggcgcc gacggtcttg acgccgcggt tacagacctc
      901 cgcggacagc tcggcgatgt cgccgacgag ctggcagccg acagtgtcgc tggtgatgtc
      961 cgggagggcg gtcggcggga tgtcgccctg ctggttctga cggaagttgt tgagcttggg
     1021 gtcgaggaag ttctgcgacc atttggcggt ctgcgggatc gtggcgtcgt cgttgacgtg
     1081 ggtgatatcg tgtacagcgg gagctatacc cccctgcgcg cccgtttaca gggcgccgtc
     1141 tcgaaactgc caaaatcgag acaaaatcgg tacggatttc gagacgtccg gcagatcgcg
     1201 ccgtcgcgca tcgccgccag gaacaggagc tagacaaggg tccgggccaa gccccggagc
     1261 cggatcgagg ccggcgatcg taccaagcgg cgaggccggc ggcgcccgca gcgcttgctc
     1321 tcgcgctggg cgacccggcg gctcaacgag cgcggagcga ggcggcggct tcgggagatc
     1381 gggttgccca tgcctcaagg gctgcgagcg tggcggcctt gaccgcgggt cgcgcggagg
     1441 agttggcgag cgatcttctg gtcgggctgc cgtagtggac atcggccagg atcaccgaga
     1501 cggccgccac gagcccgcca agcctgcgct tgcctgtacg gcgcgggagg aacgcgcgga
     1561 tcccatcgag gaggccatcg acgtgctggc catcgtcggt gcgtaggagg ttggcgaagc
     1621 tgtggatctg cggctcgctg gcgtaggggt gggtatgcca ggcgagggcg acctcgacgg
     1681 agattgggcc ctcgggggtg gggtagtcgg cgttgtggat ggcaatgtcg ccgagcgggt
     1741 cgcccagttc ggcgcggaca cagccgacaa ggcccgccgt gcgtgcagcc gtgaacgacc
     1801 acgacaggtc atggcgggga cgaggtaggt gaggtcctcg aggcggcggg tcagctccac
     1861 gcaagggacg cgagtgcagc gaacacgtgc gggtcggggc ggagacggat ggcggtgccg
     1921 gacgggcggg tcgccgggcg gacttgcaag ggggtctgca cgaggccacc ggcgtagcga
     1981 acggacgcgt gtaccccggc gtggacggtg tcgacctcaa agggatcgct gagcgcgttg
     2041 gacaccggga ggccgaggcc gatcgggctg aggtggacgt gtgggcggtg gccgtcgacg
     2101 gtcgggcggt tggacggtcg ctcgaggagc gcgacgagcc cgtgggcggg gatgccgggg
     2161 ccgtcgtcgg agatcgtgac caagtcgtcg gcgtcgacgc ggatgtcgac gcgggtgcag
     2221 cgcccgagca taacctggtc gactgcattc gagaggacct caagaacgag gtgcaatgct
     2281 ccgtcgccgg tagcggtgtc gcccgcgtac atgccggggc ggcgtcggac cgactcgcgc
     2341 agtgtgaggc tctcaatacc atccgcggtg tactcgtcgg gcatgcgtaa tcagattcta
     2401 cccgaggaga agcggagcaa gggcagcggc gggtcgcgcc gcatccacct caccctgctc
     2461 gagggcagcg gcagcccgca tcacttccac ccgacggcca gcggatccat acggcgacat
     2521 atcacgaacc gcgccgtggg ggccgatggt ggcgggtgac ctcgagttca tcggcccacg
     2581 cgtgtagggc ttgcgtcttc ccggtgatgc ggcgtcgggc gagttcggtc ggtacggaag
     2641 ggtgcccgtg gaagaggcgg caatcggcat agctcacgag caggcgcgac gacaggaata
     2701 ccagcagacc cagggggctg ccggcggccg cggcggcagc gagcttgtgg tggccgtcga
     2761 gcacgaccgc gacggcgcag cgctcgttgg cgtagctgcc gaaggcgagc accgtcggcc
     2821 gggcgccgga ggcgagccgt cctgcgtaat gatcgatgcg gacccggtcg ggcgcgccgg
     2881 tcagcggcaa gagcacgagg ccgagctcgc cgagcgacgc gcggtagagt agcgtcgcgg
     2941 tcggctgagg gacgtcgcgg ccgtagccga ttcgcacctg ggtaccggcc tcgcggacct
     3001 cgacccccca cgcgtcggca ccggcctcgg cccggtagtc ggatggacaa ttgacgcaaa
     3061 tgcctgtgtg acggacgccg gtcacgggcg cttccgcgtc gatgacatcg tagaggcccg
     3121 cgggtagcgg cgcgccggcg gctcgcagca gttcgccgtc gagctcggcc gggtgatcac
     3181 tgttcagctg cctgcgtagt tcctcggaga cgtccggagg cacagtgtcg ccgaggcagc
     3241 gaaagaagca ctgaaaggtc tcttgcgtca gctcgacgac ggtcgcgccg tgcaccgaga
     3301 ttgccccgag ctcgagcatc tcgacctgga ggtcgacgag gcccgcaccg gcgggcacag
     3361 cgtagtcagc gagcagacgg ggcaccgtgg ataggctaac actcgcggag ccggggcgat
     3421 cgcgcagaac gccgtcaatc cgtctcgtcg tcgtcgccaa cgcggtcatg gcggcgccac
     3481 ttcggaagcg cggcgagaat attcgcggcc gcgagctcgt gcacccgcgc gagcagccat
     3541 gtcatcgcgg cctcgtgctc gaggtcccct tggatcgcca gcgacgctcc tatccacggc
     3601 gacacgcggt tccactcgac ggtccagcct ggcggcaaac cggggacgag cgccgcgatc
     3661 gcgtcgtagg cgacgtggtc gccgggttga aattcgatcc atagggtcag ctccgggctg
     3721 tcgcggaagt agatcccgaa gcacacccat gggtaacgcc gacttccgac cacgaggcgg
     3781 ttgtggtgga agagctgcac cgctaccgcg cgcgagatct tcgcctcgct gcgtgggaag
     3841 ttcgactcgt gccaggccac cggcaggtcc ggccggccaa ggaattcgtg gacgagccgg
     3901 gtcgccttgc gcagcagccc gcgtgcgagc ccggcggcgc cgagctgcgt ggccgtgaag
     3961 ggtgcatcca tgtcgtcggc cattcggtgg tcctccaaaa aatcgcgcag ttcgagccag
     4021 gtatagtggc agtccacggg gatgcacgcg cgcaggcggt tccaccgcaa tgtcggcacg
     4081 tcccgggtta gcggatattt gcacagcatg aacacgaccg gctgtggtgt ggacggccaa
     4141 gcggccgctg ccgcggcgta ggcactcagc tgagctccgc tgagtccggc gtgcgctttg
     4201 acctcgatcc acagatgcag cggcgcaccg tcaacgatca cctcgacgac gaggtcgacg
     4261 atcccgccag ggacgctcac ctgggtgtcg acacggaggt cgcggaactc gctgaacgcc
     4321 gtgaggtcgg cgacctcccg gggcaacgtg cgcagcagcg ggccggggtc ttgctcgatg
     4381 gcacaggcga gcgcctcggt ggtgaagttc tcgagcgggc tgttctcggc ggcgcgcgca
     4441 aaagtgaaca gacggtcgag gcggcggcgc atcggcggcg aatatatcag cctcgccctg
     4501 gccatacgcc gtggactgct ggacctcgcc gccccgatgg ggagtggaac cgattccact
     4561 tggatccggc ttcagtagcc gactaggggt ataacagcgt tcttgaggcc gggatattgg
     4621 tgatatcgtg tataatgggc ccgagactcg tctacggcac cgtatacgag gaactgcctc
     4681 ggaaaccacc aggatcgcga caaaatcggt acgaattttc gagacgtccg ggagatcgcg
     4741 ctgtcgcgca tgcccacctc agacagcagc tagagggggg ttcggcgcaa gccccggagc
     4801 cggatcgagg ccgacgatca tgccacgcgg cgaggccacg gggcggccta tgcctgtccc
     4861 gaaggacagg aagacgacga accccgtgac acctcgagcg tgctcgtcga cgcggcggtc
     4921 tcaccgtcgc caccgtcacc gcgcccccgc gcgagcacca gcgcctggct aggccatgaa
     4981 accgagcagc gggtcatcct ttagcagcgg tatcgacgta gtccacctgc gacgccggct
     5041 tgaacgcctt ggaacggccg agcaacgtga ccactggttg tgctgccgag ccggcccatc
     5101 tgcgccgcga aggccgaccg ctcgccgtcg gtggtaggat gcggtcggca tggcccgctt
     5161 gtcgaagacc gacgcgcacg cggtgttgtc cttcttcatt cctgttcttc acgttctccg
     5221 ctctgctcga cccgctcgtc atcccggcta ccgctcagct cgtggccgct gcttggatca
     5281 acattgctac ctgtttctgg gacatttaga gcatcttggt caaaagccat gaacagcgtc
     5341 cgtgtagact cgacaatgct gggataggcg tgactcacca cctcgagtgc ccgcttgaca
     5401 tcgtgatagc catcaatgct tgagtcgaca ctcagccacg ctagagaaat tatggccagt
     5461 tcaacgacct ccggactcgc tacgccaaga tcagcatgga gcgtccaatc tatgagattc
     5521 gagacggtgt cccggagttc tgaggttcta attgccgaca gcggacggac ccaaaaattt
     5581 gtgcggtcct ctatcgacag ggcgcgcagt cgctcgtgca gagcacgagc attccaggga
     5641 tggctcggga cgcaagcagc agagagaagg acttcgatct tctcttccgg agaggcatca
     5701 aacaagtcat tcaagatctg ccgcgttctc tgtgtaatgg cattttggcg ccgatcgcgc
     5761 acggattcac ggaagacccg tttaaactga tgggttttga gcaaccgacc gtcgtcattg
     5821 aagaaactag ctaggtcgac aagctccttt ccgtggacct cggccaaagc actccagatt
     5881 gccgaaacga ctccagccca ttgctggtgt atgtgaacga cgttctttcc tcgttccgga
     5941 attcgctgta tgaggaagga aagcatggct ccgtcggcga acgctcgttc gacgtcgttg
     6001 catcgggcca agagcgatcg cgcgatcaag ttgtcctgca atcgctggaa agcgaaacgg
     6061 acgacctccg acgggttgga ccagtcgtcc acaggacctt cccgcggggc ggtgtcgcgc
     6121 cgaagaagat ctgatccttc aagtacctcg agccacgcac gcccttcgag cggaaagttg
     6181 gcgaacgtgt ctgcaaggat tgccaaagct tccccgacag ggacagcgtc agcggtgtgg
     6241 tcggccatcc attgcgcgag gagcgatagc gcagccttca ctgcattgtg tagagattcc
     6301 tgaccgtcgt agc
//
LOCUS       DASWNJ010000272         2665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_320954, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000272 DASWNJ010000000
VERSION     DASWNJ010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320954"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VGB85_04940"
     CDS             <1..770
                     /locus_tag="VGB85_04940"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY0133398.1"
                     /translation="MFVDIVGFTAMAESMSSTETFNLINDYLHHVGPKIREHGGFVDK
                     YIGDAIMAIFLRPVEAVDAAVALLGALRDFNAGRLRDGQSTLGIGIGIHHGMMSFGIL
                     GEQQRMQSTAIADAVNVAARIEGLTRCYGAGILLSEAARGQLASDGSRAYRFIDHVQL
                     RGRREELLVWEALAPLEIPEHGARVEQLETYNHGIRAFGEGRWSDAHALFRQIVDRDP
                     HDGTARSYLERAACRVASGEDPLHRLAEDRRFQAFEE"
     gene            859..>2665
                     /locus_tag="VGB85_04945"
     CDS             859..>2665
                     /locus_tag="VGB85_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEY0133399.1"
                     /translation="MMNTTDAMVVRADVIGLHEVDRTRASLVGGKGANLGELARIAGV
                     RVPAGFCVTTGAFARVVAGTPAVHGMLDRLASLTAQDRDGIRELGGELRRIIEATAIP
                     DDLETAIARMLSRLGADETYAVRSSATAEDLPGASFAGQQDTYLEIRGTPAILGHVRR
                     CWASLFSDRAVAYRIQHGFDHRKILMAVVIQKMVRAQAAGILFTADPVTSNRKVTSIE
                     AGLGLGEALVAGLARADRYRVRDGQIVEKAVASEALTEAQVLRLERLGRQIEAHFGEP
                     QDVEWCLFEGEFDVVQSRPITTLYPIPGADDGDNRVYISVGHQQMMTDAMKPLGLSLF
                     RLSSGGHMHAAGGRLFVDATKQLASPTSRDALLGLMARSDPLTRDALLQILERADFLK
                     VSPEAPRQGAGRFDVHVDDDPAIVAELIDHSQAEIEALRREIRTKTGTALFDFISEDI
                     LRLKQSLFAPRSMGVIMAGMNALWWINEQMLAWLGEKNVGDTLSQSVANNVTSEMGLA
                     LLDVADVIRPYPDVVAFLQHVKHDGFLDEMVALEGGQQARDAIRAYLDKYGMRCVGEI
                     DITRARWSERPTTLVPAILNNVKNVEAGAGARRFAQ"
BASE COUNT          445 a          842 c          946 g          432 t
ORIGIN      
        1 tcatgttcgt cgacatcgtc ggctttaccg cgatggccga gtcgatgtcg tcgaccgaga
       61 cttttaatct gatcaatgat tatctccacc acgtcgggcc caagatccgc gagcacggcg
      121 gatttgtcga caagtacatc ggcgacgcga tcatggcgat cttcctccgc ccggtcgagg
      181 cggtcgacgc cgcggtcgcg ctgctcggcg cgctgcgaga cttcaacgct ggccggctcc
      241 gcgacggcca gtcgacgctc ggcatcggca tcggcattca tcacggcatg atgtccttcg
      301 ggatcctcgg cgagcagcag cgcatgcaga gcaccgcgat cgccgacgcg gtcaacgtgg
      361 ccgcccgcat cgaggggctc acgcgctgct atggcgcagg catcttgctc agcgaggccg
      421 ccaggggcca gctggcgagc gacggctcgc gtgcctatcg cttcatcgat cacgtgcagc
      481 tccgcggccg gcgcgaggag ctgctcgtgt gggaagccct cgctcccctc gagatcccgg
      541 agcacggcgc gcgcgtcgag cagctggaga cctacaatca cggcatacgg gccttcggcg
      601 agggccgctg gtcggacgcc cacgcgctgt ttcgccagat cgtcgaccgc gatccgcacg
      661 acgggaccgc gcgctcgtac ctcgagcgtg ccgcctgtcg cgtggccagc ggcgaggacc
      721 cgctgcatcg cctcgccgag gaccggcgtt tccaggcctt cgaggagtga cgcgcaagca
      781 cgtccctctc caccacgagg ttataacagg cacgggggct tgcagcaagc cccccggcag
      841 gccgcacagt ccccggccat gatgaacacg acggatgcaa tggtggtgcg agccgatgtg
      901 atcggtctgc acgaggtcga tcgaacgcgc gccagcctgg tcggggggaa gggcgcgaac
      961 ctgggggagc tggcgcggat cgcaggggtc cgcgtgcccg ccggcttttg tgtcacgacc
     1021 ggggcctttg cgcgggtcgt ggcaggaacg ccggcggtcc atggaatgct cgatcgcctg
     1081 gcgtcgctca cggcgcagga ccgggacggg atccgcgagc tcggcggcga gctgcgccgg
     1141 atcatcgaag cgacggccat cccggacgac ctcgagacgg cgatcgcccg gatgctctcc
     1201 cggctcggcg cggacgagac atatgcagta cgctcgagcg cgacggcgga ggatctaccg
     1261 ggggcctcct tcgcgggcca gcaggacacg tatctcgaga tccgtggcac accggcgatc
     1321 ctcgggcacg tgcgccggtg ctgggcgtcg ctgtttagcg atcgcgcggt ggcctaccgg
     1381 atccagcacg gcttcgacca tcgcaagatc ctcatggcgg tggtaattca gaagatggtc
     1441 cgcgcgcagg cggcggggat cctgttcacg gccgaccccg tgacgtcgaa ccgcaaggtg
     1501 acgtcgatcg aggccggcct gggccttggg gaggctctgg tcgccggcct ggcgcgcgcc
     1561 gatcgctaca gggtgcgcga cggacagatc gtcgagaagg cggtagcgag cgaggcactg
     1621 acggaggcgc aggtgctccg gctcgagcgc ctgggccggc agatcgaggc gcacttcggc
     1681 gagccccagg acgtcgaatg gtgcctgttc gagggtgaat tcgacgtggt ccagagtcga
     1741 ccgatcacga ccctgtaccc gatccccgga gcggacgacg gggacaatcg ggtctacata
     1801 tcggtcggcc accaacagat gatgaccgac gccatgaagc cgctcgggct gtccctgttt
     1861 cgcctgtcct ccggcgggca catgcacgcg gcgggaggca ggttgttcgt cgatgcgaca
     1921 aagcagctag cctcaccgac gagtcgtgat gctctcctgg gtctgatggc tcgctccgat
     1981 ccgctgacca gggacgccct cctgcagatc ctcgagcggg cagacttttt aaaagtgtcg
     2041 cccgaggcgc cgaggcaggg ggctgggcgt ttcgacgtgc atgtcgacga cgatccggcg
     2101 atcgtggcgg agctgatcga tcacagccag gccgagatcg aggcgctgag gcgggagatc
     2161 cggacaaaga ccgggacggc gctgttcgac ttcatctcgg aggacattct gcggctgaag
     2221 cagtccctgt tcgccccgcg gagcatgggc gtgatcatgg cgggtatgaa cgcgctgtgg
     2281 tggatcaacg agcagatgct ggcctggctg ggcgagaaga acgtcgggga tacgctgtct
     2341 cagtcggtgg cgaacaatgt cacgtcggag atggggctgg cgctgctcga tgttgccgac
     2401 gtgatccgcc catatccgga cgtggtcgct tttttacagc acgtgaagca tgacggcttc
     2461 ctggatgaga tggtcgcgct cgagggaggg cagcaggccc gggacgcgat ccgggcgtac
     2521 ctcgacaaat atggcatgcg ctgtgtcggg gagatcgaca tcacgcgggc ccgctggagc
     2581 gagcggccca cgaccctggt gcctgcgatc ctcaacaacg tcaagaatgt cgaggcgggc
     2641 gccggcgcgc ggaggttcgc gcagg
//
LOCUS       DASWNJ010000273         3206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_641377, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000273 DASWNJ010000000
VERSION     DASWNJ010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641377"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            326..754
                     /locus_tag="VGB85_04950"
     CDS             326..754
                     /locus_tag="VGB85_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133400.1"
                     /translation="MLSTITLLASTLAFSPALSVAPVMATSGYNPGGINIGIGGGGGD
                     IDLDLDLDACLDLDLDIDLDCEIEVGAECHADCEVNVDAYCDAQANANKWNAKAHAQC
                     EAEVEAQCHANCDIDGSVFCEGDIYVGADICLDVSLDLDL"
     gene            1526..1957
                     /locus_tag="VGB85_04955"
     CDS             1526..1957
                     /locus_tag="VGB85_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133401.1"
                     /translation="MLSTITILVSTLAFAPTYTAAPVESSSRTPGGIGIGLGGGGGGG
                     DIDVDLDACLDLDLDVVLDCKVKVGAQCHAACEVNVDAYCDAQVDADEWDAKAHAQCE
                     AEVEAQCHANCDIDGSVFCKADIYVGADICLDIGLDLDLDL"
     gene            2175..>3206
                     /locus_tag="VGB85_04960"
     CDS             2175..>3206
                     /locus_tag="VGB85_04960"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0133402.1"
                     /translation="MAVHDAPVAGDRLGRFVVLERLGEGGMGVVYSAYDTTLERKVAV
                     KLLRDLDQSGDVRNLRAWREALALARLSHPNVIQIYDVGTHAGKVFLAMEFVAGADLA
                     AWLAAAPKSVAEILDVFLAAGRGLAAAHAAGLVHRDIKPANILLGNDGRVRVLDFGLA
                     RSVHGEAEVPAAVPVPVSVPVDTTSVLTHPGMNPGTPRFMSPEQREGGLLTPSSDQYS
                     FCVALDGALATAMPSPRTRAELVRLQAVLDRGMREAVDQRFATMDDLLAALMAARARP
                     HRRGLVVAGLLACLGLGVYVASTAAAPSLCAGGPARVAAVWHPDRALALREHLAAGSA
                     DLGDRARDAI"
BASE COUNT          484 a         1305 c          928 g          489 t
ORIGIN      
        1 gcgatctatt tctagaacct cgtgcggcac gatcgacaca cgatgcgttt tgaaatgcgg
       61 ccatttaccc gcgttcaagc agcgtcttcg cgcagctcgc cgccttcaaa aaaatcctga
      121 atttcctccc agcgaccacc acagcgctgc caaacgcctg accatcgccc ggcgccgccc
      181 cggatccgac ctcgatcgcc gctctcgtcg tggccgcgaa atctccggcc cgcatactcg
      241 actcatcgag ccgcgacgga ctcccccggc ggccctcaag acactcactg ccccgacgat
      301 gttcggggaa aaaggcccac accccatgct ctccaccatc accctgctcg cctccaccct
      361 cgccttctcc cccgccctct ccgtcgcccc cgtcatggcc acctccggct acaacccggg
      421 cggcatcaac atcggcatcg gcggcggcgg tggtgacatc gacctcgacc tcgacctcga
      481 cgcctgtctc gacctcgacc tcgacatcga cctcgactgc gagatcgagg tcggcgccga
      541 gtgccacgcc gactgcgagg tcaacgtcga cgcttactgc gacgcccagg ccaacgccaa
      601 caagtggaac gccaaggccc acgcccagtg cgaggccgag gtcgaggccc agtgccacgc
      661 aaactgcgac atcgacggct ctgtcttctg cgagggcgac atctacgtcg gcgccgacat
      721 ctgcctcgat gtcagcctcg acctcgacct gtaagccttc cccggctctc ccgaacgggc
      781 gcccacgcac tgcgtcggcg ccctttctgc gttcgtcagc ctcgaccgcc aaacgggcgc
      841 cacgcactgc gtcggcgccc gttctgcgtt cgtccgtcca cctcgaccgt cgccgaacgg
      901 gcgcccacgc actgcgtcgg cgccctttct cgttgccccg agctcggccc ggacgccaga
      961 acgcagcttg ccgacatggc ccccgatcag ggtcctccca gacacgacat cgcggcgccg
     1021 gcgtccccaa gaaacctgcc gcgcatgcac acatacatgc ccgacgcttg ttgcatttcc
     1081 tcgacacccg gtcaccgcca cgctcctgcg ccgcggtccg cgccaccaac ctcctcaaat
     1141 cacctgagat ctcgatgact tgcacgcaac ccaacgaacg ggagcgcccc gccggtgtac
     1201 gttatgcgat ctattcgaac gtctgcgtgc agcgagatcg acacacgctg ggctttgcga
     1261 ggcggccttt ttggtgcagt tccggcagcc ttttggcccc tgctcgggcc acgaaaattt
     1321 ccacaaaatg cctcctgcga ccttcctgat cccgcctaca ggcaaacgag cccagcgcgc
     1381 cgcacctcgc tggcgagcat tcgccttcga cgatatggcg acaccgtcat cggcccgcat
     1441 actcgactca tcgagccgcg acggaatctc ccggcggccc ccaagacgct ctccccgacg
     1501 ctgtcgggca caaaggacca ctcccatgct ctccacgatc accatcctcg tctccaccct
     1561 cgccttcgcc cccacctaca cggccgcgcc cgtcgagagc tccagccgca ctcccggtgg
     1621 catcggcatc ggcctcggcg gcggtggtgg cggtggtgac atcgacgtcg acctcgacgc
     1681 ctgtctcgac ctcgacctcg atgtcgtcct cgactgcaag gtcaaggtcg gcgcccagtg
     1741 ccacgccgcc tgcgaggtca acgtcgacgc ctactgcgac gcccaggtcg acgccgacga
     1801 gtgggacgcc aaggcccatg cccagtgcga ggccgaggtc gaggctcagt gccacgccaa
     1861 ctgcgacatc gacggctccg tcttctgcaa ggccgacatc tacgtcggcg ccgacatctg
     1921 cctcgacatc ggtctcgatc tcgatctcga cctgtaaccc tcccttccat catcccgaaa
     1981 aggcgcccac gcactgcgtg ggcgcctttt cggcgtgcgg gcgaaggtga gcagcgcggc
     2041 gcgccgcgga cggtggtcat cacccgccgc gacccggacg ggattatgct cgcgggacgg
     2101 aggacgagac gacagatggc cgcgagcccg gcggcgcccc ggacgtcacc ctcgtccgct
     2161 cggacgtccc gtggatggcg gtgcacgacg ccccggtcgc gggcgaccgc ctggggagat
     2221 tcgtcgtgct cgagcggctc ggggagggcg gcatgggcgt ggtgtactcg gcctacgaca
     2281 ccaccctcga gcgcaaggtc gccgtcaagc tgctgcggga cctcgaccag tccggcgacg
     2341 tacgcaacct ccgcgcctgg cgcgaggccc tcgcgctcgc ccgcctgtcg caccccaacg
     2401 tcatccagat ctacgacgtc ggcacccacg ccggcaaggt gttcctggcc atggagttcg
     2461 tcgccggcgc cgacctggcg gcctggctcg ccgcggcgcc gaagtccgtg gccgagatcc
     2521 tcgacgtgtt cctcgccgcc ggtcgcgggc tcgcagcggc gcacgccgcc ggcctcgtcc
     2581 accgcgacat caagcccgcc aacatcctcc tcggcaacga cggccgcgtc cgcgtcctcg
     2641 acttcggcct cgcacgcagc gtccacggcg aggccgaagt ccccgccgcc gtccccgtcc
     2701 ccgtctccgt ccccgtcgac accacgtcgg tcctcaccca ccccggcatg aaccccggga
     2761 ccccgcgctt catgtccccc gagcagcgcg agggcggcct gctcaccccg agcagcgacc
     2821 agtactcgtt ctgcgtcgcc ctcgatggcg cgctcgcgac cgcgatgccc tcgccccgga
     2881 cccgcgccga gctcgtgcgc ctgcaggcgg tgctggaccg cggcatgcgc gaggccgtcg
     2941 accagcgctt cgcgacgatg gacgacctgc tcgccgcgct catggcggcc cgcgcccgcc
     3001 cgcatcgccg gggcctcgtc gtcgccggcc tgctcgcctg cctcggcctc ggcgtgtatg
     3061 tcgcgagcac cgccgcggcg ccgtcgctgt gcgccggcgg ccccgcccgc gtcgccgccg
     3121 tgtggcaccc cgaccgcgcg ctcgccctcc gcgagcacct cgccgccggg tccgccgacc
     3181 tcggcgaccg cgcccgcgac gcgatc
//
LOCUS       DASWNJ010000274         4197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_181721, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000274 DASWNJ010000000
VERSION     DASWNJ010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181721"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VGB85_04965"
     CDS             complement(<1..654)
                     /locus_tag="VGB85_04965"
                     /inference="COORDINATES: protein motif:HMM:NF014598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HEY0133403.1"
                     /translation="MHALWFMLLALAVLAIAYRYYSAFLAARVLSLDPGRVTPAHVHN
                     DGQNYHPTHRWVLFGHHFAAITGAGPLVGPVLAAQFGFLPGYMWILFGVVLGGAVHDF
                     VILTASVRRGGRSLAEMARDELGPGVGAVAGLAILFIVIIALAGLGRVVVGALAESAW
                     GVFTIGASIPIALAMGLYIYKFRGGSVKGVREATVVGVTLLLLCVVFGKQVQDSSFGA
                     "
     gene            complement(692..1558)
                     /locus_tag="VGB85_04970"
     CDS             complement(692..1558)
                     /locus_tag="VGB85_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0133404.1"
                     /translation="MHLRPLGSSGLHVSQIGFGGMPLSIAGRPARSEAIKVLHASLRA
                     GVTLIDTADVYCLDQSDINHNEKLIAEALAQLPSPGNVVVATKGGLQRPGGAWVTDAH
                     PRRLRSACEASLKALRLPVIPLYQLHAVDDAVPLEDSVGALAELRRQGKIAHIGLSNV
                     TPAELKRALAIVPIVSVQNRCSVFDRRDFSNGMVALCTAEKIAYLAYSPVGGGRGKVR
                     VADDPTLRAVGARHDVSPFQVALAWLLARSPMMIPIPGASRISSALDSAAADELQLTA
                     EDHATLDQAFPS"
     gene            1790..2476
                     /locus_tag="VGB85_04975"
     CDS             1790..2476
                     /locus_tag="VGB85_04975"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY0133405.1"
                     /translation="MDPTIILTVTVAVTALVLGLNFAMIARWYRRVPANAVLVVTSQQ
                     GVRVLRRGGVVLPIIHGAESLDLGPRTIDIRRVGREGIVCRDNIRVDVTATFTLAVRD
                     SVEDITKIARSIGCAGASDPEVLRKLFTVRLEDAVREVLRNHDYAKILSLRSELRDQI
                     LDNLGEAIDGFELKAVALGPLEPTPVDQLDRENIIDAEGIRRMTEIVCAAGIATSELQ
                     QRLKTVLASA"
     gene            complement(2507..4066)
                     /locus_tag="VGB85_04980"
     CDS             complement(2507..4066)
                     /locus_tag="VGB85_04980"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme peroxidase family protein"
                     /protein_id="HEY0133406.1"
                     /translation="MEDVRLQGPEDARMFQDALYPGRSADSKTASCTDADDYGPMFKD
                     LPPFADPNDPKVLAKIRELGAPGGLMDARDPVVTIVNPLDANPKFNPDNSNPEMTVGF
                     TFLGQFLDHDITFDPSPLHMPATTGPNLRSPFLDLDSVYGRGPDEDRILYDRSSELER
                     GVPAKFLIDFDAPNDLPRTSQQRAIIADPRNDENVIVSQLHLAMLKFHNRVVEHVVEQ
                     AKAGETAKELFTRAQQLVRWHYQYIVVNQFLRVSLDPTVYADVRERGPKLFKVSPTPT
                     LPREFQVAAYRFGHSQIRPGYQVNVGFGAPIFDAEIDPRSGDPNDLRGGRRADRRFVE
                     WDTFFDFGTLEVAAVQAAPGAAPTAVARPKVKRNKRIDPFISTPMFKLPVGPGLVPVG
                     DTHPSLPGRNLERHLQHGLPSGQAIAKKLGYAALSPADLDELAPLGFHTSTPLWYYIL
                     KEALLQHKGQRLGQVGSRIIAEVFIGLLLSDKGSYLSKQPGWQPELPRRDGSQTGPFT
                     MVDLLSFAGVA"
BASE COUNT          668 a         1480 c         1423 g          626 t
ORIGIN      
        1 cgccccgaag ctcgagtcct ggacctgttt gccgaatacg acgcacagca gcagcagcgt
       61 cacgccgacg accgtcgcct cgcgcacccc cttcacgctg ccgccgcgga atttatagat
      121 atacaggccc atcgccagcg cgatcgggat cgacgcgccg atcgtgaaca ccccccacgc
      181 gctctccgcc agcgccccga ccacgacccg tccgagcccc gccagcgcga tgatgacgat
      241 gaacaggatc gccagccccg cgaccgcgcc gactcccggc ccgagctcgt cacgcgccat
      301 ctcggcgagc gagcgcccgc cgcgacgcac cgacgccgtc aagatcacga agtcgtgcac
      361 cgccccgccg agcaccaccc cgaacaggat ccacatgtag ccgggcaaaa acccgaactg
      421 cgccgcgagc accggaccga ccagcggacc cgccccggtg atcgccgcga agtggtgccc
      481 gaacagcacc cagcggtgcg tcggatgata gttctgcccg tcgttgtgca cgtgcgccgg
      541 cgtcacccgc cccgggtcga gcgacagcac ccgcgccgcg aggaaggccg agtagtagcg
      601 atacgcgatg gccagcacgg ccagcgccag gagcatgaac cacagggcgt gcatcgcccc
      661 ccaggattaa cacaaaccgc gcgcgctcac atcagctcgg gaaggcctgg tcgagggtcg
      721 cgtggtcctc ggcggtcagc tgcagctcgt cggcggcggc gctgtcgagg gcgctgctga
      781 tccggctggc gccggggatc gggatcatca tcggcgagcg ggccagcagc cacgcgagcg
      841 cgacctggaa cggtgatacg tcgtggcggg ccccgaccgc gcgcagggtc gggtcgtccg
      901 cgacgcggac cttgccgcgg ccgccgccga ccgggctgta agccaggtag gcgatcttct
      961 ccgccgtgca cagcgcgacc atcccgttgc tgaagtcgcg gcggtcgaac acgctgcagc
     1021 ggttctgcac gctgacgatc ggcacgatcg ccagcgcccg cttgagctcc gccggggtca
     1081 cgttgctcag gccgatgtgc gcgatcttcc cctggcggcg cagctcggcg agcgccccga
     1141 cgctgtcctc gagcggcacc gcgtcgtcga ccgcgtgcag ctgatacagc gggatcaccg
     1201 gcagccgcag cgccttgagg ctcgcctcgc aggccgaacg caggcgccgc gggtgcgcgt
     1261 cggtcaccca cgcgccgccg ggccgctgca gcccgccctt ggtcgcgacc acgacgttgc
     1321 ccggcgaggg cagctgcgcg agcgcctcgg cgatcagctt ctcgttgtga ttgatgtccg
     1381 actggtcgag gcagtacacg tccgccgtgt cgatcagcgt cacgccggcc cgcagcgacg
     1441 cgtgcagcac cttgatcgcc tccgatctcg cgggtcgtcc ggcgatcgag aggggcatcc
     1501 cgccgaagcc gatctgggac acatgaaggc cggagctgcc gagcggacga aggtgcatac
     1561 ggactgtcgg ccctcataac acaactcgcg gggcggtggc cgcgggcgtc ctccacatga
     1621 tccagatgat ctcgcccagg cacaccacgg ccacacatca cggccgcaac cgcacgccag
     1681 cggccccgcg cgcgcggacc cgaccgcgag gcacacacgc gcacgcgaca gcgcccgatc
     1741 gatcatggta tctcgcgggg tgggccatca cacgcgcccg cggtgaaaca tggatccgac
     1801 gatcatcctc acggtcacgg tcgcggtcac ggccctggtc ctcggcctga acttcgccat
     1861 gatcgcccgc tggtaccgcc gggtccccgc caacgccgtc ctcgtcgtca ccagccagca
     1921 aggcgtccgg gtgctgaggc gcgggggcgt ggtcctgccg atcatccacg gcgccgagtc
     1981 gctcgacctc ggcccgagga cgatcgacat ccgtcgcgtc ggccgcgagg gcatcgtgtg
     2041 tcgtgacaac atccgcgtcg atgtcaccgc caccttcacc ctcgccgtgc gcgacagcgt
     2101 ggaggacatc acgaagatcg cgcggtcgat cggctgcgcc ggcgcgagcg accccgaggt
     2161 gctgcgcaag ctgttcaccg tgcgcctcga ggacgccgtg cgcgaggtcc ttcgcaacca
     2221 cgactacgcg aagatcctgt ccctgcgctc cgagctgcgc gaccagatcc tcgacaacct
     2281 cggcgaggcg atcgacggct tcgagctcaa ggccgtcgcc ctcggcccgc tcgagccgac
     2341 gccggtcgat cagctcgacc gcgagaacat catcgacgcc gagggcatcc gccggatgac
     2401 cgagatcgtg tgcgcggccg ggatcgccac cagcgagctc cagcagcgac tcaagaccgt
     2461 cctcgccagc gcgtgaacgg gggccgagcc cggcggcccc gcttgctcag gcgacgccgg
     2521 cgaagctcag caggtcgacc atcgtgaatg gaccggtctg cgacccgtcg cggcgcggga
     2581 gctcgggctg ccagcccggc tgcttgctca ggtaggagcc cttgtcgctc agaagcagcc
     2641 cgatgaacac ctcggcgatg atgcggctgc cgacctgacc gaggcgctgg cccttgtgct
     2701 ggaggagcgc ctccttgagg atgtagtacc agagcggcgt gctcgtatgg aagccgagcg
     2761 gcgcgagctc gtcgaggtcg gccggcgaga gcgcggcgta acccagcttc ttggcgatcg
     2821 cctggcctga gggcaggccg tgttgcaggt ggcgctcgag gttacgtccg ggcaggctcg
     2881 ggtgcgtatc gccgaccggg acgaggcccg ggccgaccgg cagcttgaac atcggcgtcg
     2941 agatgaacgg gtcgatccgc ttgttgcgct tgaccttggg ccgggcgacg gcggtcggcg
     3001 ccgctcctgg tgcggcctgg accgccgcga cctcgagcgt gccgaagtcg aagaaggtgt
     3061 cccactcgac gaagcgccgg tccgcccgac ggccgccccg gaggtcgttc ggatccccac
     3121 tacgcgggtc gatctcggcg tcgaagatcg gcgcgccgaa gccgacgttg acctggtacc
     3181 cggggcggat ctggctgtgg ccgaagcgat aggcggcgac ctggaactcg cgcggcagcg
     3241 tcggcgtcgg cgagaccttg aacaacttgg gaccacgctc ccggacgtcg gcgtagaccg
     3301 tcgggtcgag gctgacccgc aagaactggt tgaccacgat atactggtaa tgccagcgaa
     3361 cgagctgctg cgccctcgtg aacagctctt tcgcggtctc gccggccttc gcctgctcca
     3421 ccacgtgctc gaccacccgg ttgtggaact tgagcatcgc caggtgcagc tgcgacacga
     3481 tcacgttctc gtcgttgcgt ggatcggcga tgatcgcccg ctgctggctg gtccgcggca
     3541 ggtcgttcgg cgcgtcgaag tcgatgagga acttggccgg caccccgcgc tcgagctcgc
     3601 tcgatcggtc gtagaggatg cggtcctcgt cgggcccgcg gccgtagacg ctgtcgaggt
     3661 cgaggaacgg gctgcgcagg ttcggccccg tggtcgccgg catgtgcagc ggggacgggt
     3721 cgaaggtgat gtcgtgatcg aggaactggc cgaggaaggt aaatccgacc gtcatctcgg
     3781 ggttgctgtt gtccgggttg aacttcgggt tggcgtcgag cggattgacg atcgtgacca
     3841 ccgggtccct ggcgtccatc aggccgcccg gcgccccgag ctcgcggatc ttcgccagca
     3901 ccttcggatc gttcggatcg gcgaagggcg ggaggtcctt gaacatcgga ccataatcgt
     3961 cggcgtcggt gcaggacgcc gtcttgctgt ccgcgctgcg cccgggataa agagcgtcct
     4021 ggaacatcct tgcgtcctca ggaccctgta agcgtacgtc ttccatatat tctccggtgt
     4081 gcgccgactc gggatgaata cccgtcgctt cggcgtcgac cagtcagtgt cacgcggcgc
     4141 agccatcgat cactccgaca gcgatggccc gcgtcggctc ggacctggag cttggtt
//
LOCUS       DASWNJ010000275         1736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_111221, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000275 DASWNJ010000000
VERSION     DASWNJ010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111221"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..733
                     /locus_tag="VGB85_04985"
     CDS             62..733
                     /locus_tag="VGB85_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133407.1"
                     /translation="MNALAGALLLVGAEVASLVTTGSHVAGARLVGGVLGVTDWGVAF
                     AGVAEGLAAFGERRRALTPEEYAWAGRVFAGTLPPRERLVVTDTIGAGERPFTFPRLD
                     GTITLNLGPEGFADPRRDIFGSGAARGETFVHELAHAWQVAHHSPELVLLAGGVACRL
                     RELVGVDPYVYSPGRAFGEYTLEQQAQIVSDWFGRHHRRLRSATARRDPRYRYIEGNI
                     RRGRR"
     gene            complement(749..1720)
                     /locus_tag="VGB85_04990"
     CDS             complement(749..1720)
                     /locus_tag="VGB85_04990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03885 family FMN-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEY0133408.1"
                     /translation="MARIGFHASHEQFAPSELLRLVKSAEAAGFGCAMSSDHFKPWGP
                     DQGHSGYAWSWLGAAMQATRLPFGVVCAPGQRYHPAIVAQAAATLEQMFPERMWLAIG
                     SGEFINEAITGDPWPLKADRNARLRECADVMRALWAGETVTHHGHVHVEEARLYTLPK
                     RPPLLVGAAVTPKTARWVGEWADALITVTRPDQGHREVAEAFREGGGAGKPMLLKAQI
                     AYAEDEQDALHGAWDQWRTNIFDSSVLADLRTVAQFDALGEFVRPEDLRGPVRISADP
                     GQHAAWLAADLELGFDVVSVHNVIKEQQRFIDVFGERVLPQLRNVAR"
BASE COUNT          235 a          570 c          682 g          249 t
ORIGIN      
        1 agtgacctgt ccttgcagac aggtgagttg ccgcggcggt gaaaccggac aagctcgggg
       61 ggtgaacgcg ctggcgggtg cgctgctgct ggtcggcgcc gaggtcgcct cgctggtgac
      121 gacggggtcg cacgtggcgg gcgcgcggct ggtcggcggg gtgctcgggg tgacggactg
      181 gggggtcgcg tttgcgggcg tggccgaggg gctggcggcg ttcggcgagc gccggcgggc
      241 gctgacgccg gaggagtacg cgtgggcggg gcgggtgttc gcgggcacgc tgccgccgcg
      301 tgagcggctg gtggtgaccg atacgatcgg cgcgggcgag cggccgttca cgttcccgcg
      361 gctcgacggg acgatcacgc tcaacctcgg accggagggc ttcgccgacc cgcgacgcga
      421 tatcttcggc agcggcgcgg cgcgcgggga gacgttcgtg cacgagctgg cccacgcgtg
      481 gcaggtcgcg caccacagcc cggagctggt gctgctcgcc ggcggggtcg cgtgtcgcct
      541 gcgcgagctc gtcggggtcg acccgtatgt atacagtccg gggcgagcgt tcggcgaata
      601 tacgctcgag caacaggcgc agatcgtcag cgactggttc ggccggcacc accggcggct
      661 gcgctcggcg acggcgcgcc gcgatccgcg gtatcgctac atcgagggca acatccggcg
      721 cggacggcga tgagaggatc gcgtgaggtc agcgggcgac gttgcgcagc tgcggcagca
      781 cgcgctcgcc gaacacgtcg atgaagcgct gctgctcctt gatcacgttg tgcacgctga
      841 cgacgtcgaa gccgagctcg aggtcggcgg cgagccacgc ggcgtgctgg cccgggtcgg
      901 cggagatccg cacgggccca cgcaggtcct cggggcgcac gaattcgccg agcgcgtcga
      961 actgggcgac cgtgcgcagg tcggcgagca cgctgctgtc gaagatgttg gtgcgccact
     1021 ggtcccacgc gccgtgcagg gcgtcctgct cgtcctcggc gtaggcgatc tgggccttga
     1081 gcagcatcgg cttgccggcg ccaccaccct cgcgaaaggc ctcggcgacc tcgcggtggc
     1141 cctggtcggg gcgcgtcacc gtgatcagag cgtcggccca ctcgccgacc cagcgggcgg
     1201 tcttgggggt gacggcggcg ccgacgagca gcgggggccg cttcggcagc gtgtaaaggc
     1261 gggcctcctc gacgtgcacg tggccatgat gggtgacggt ctcgccggcc cacagcgcgc
     1321 gcatgacgtc ggcgcactcg cgcagccggg cgttgcggtc ggccttgagc ggccacgggt
     1381 cgccggtgat cgcctcgttg atgaactcgc cgctgccgat cgccagccac atccgctcgg
     1441 ggaacatctg ctcgagggtc gccgcggcct gggcgacgat cgcagggtgg tagcgctggc
     1501 ccggcgcgca cacgaccccg aacggcagac gcgtcgcctg catcgccgcg ccgagccacg
     1561 accaggcata gcctgagtgg ccctggtccg gcccccatgg cttgaagtgg tcggacgaca
     1621 tggcgcagcc gaagccggcc gcttccgccg atttaaccag gcgaagcagt tccgatggcg
     1681 cgaactgctc gtgcgatgcg tgaaagccga tccgggccat gggacactcc gcagac
//
LOCUS       DASWNJ010000276         1589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_321044, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000276 DASWNJ010000000
VERSION     DASWNJ010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321044"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="VGB85_04995"
     CDS             complement(<1..517)
                     /locus_tag="VGB85_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133409.1"
                     /translation="MVEPRWHAARRSAAQDLGGRGGRLLGLVVALLTGCTANPGPREC
                     TLIGCMSGLSIGLEKASPWAAGAYTFDFDLDGVVVTCDGSLPRRGCEGGPSATCEPPE
                     QVSAPGTGCVVMHGMHGFDGVAVAGAPRTVVLKISRDGQTLHTAQLTPTYKRSQPNGE
                     GCPSICDNAYEI"
     gene            complement(836..>1589)
                     /locus_tag="VGB85_05000"
     CDS             complement(836..>1589)
                     /locus_tag="VGB85_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133410.1"
                     /translation="EMPDADTLLLSGERDDNIWYQRGARDGTAWLPAWDPEPVPFDAD
                     ANFNNPAIAQAITALPDAVIVGGSWNVAGDNVYSAWLRRLGPADGAVACPCELANAGV
                     MAMAPAGMDAVHVAGFAQDGTQTQLWLAKLGATCPMNCVPVWEEKVPGKFADAYVLRA
                     ELLRDLAYTIVPLADGGVIVGGTVDSKPWAALRAADGTWPWTMEPSPNVALGAVMAAS
                     LSADERCLTLAGSKDHLELGARQWWVRRIPLP"
BASE COUNT          233 a          616 c          504 g          236 t
ORIGIN      
        1 ctatctcgta cgcgttgtcg cagatcgacg gacagccctc cccgttcggc tgcgacctct
       61 tgtaagtcgg cgtcagctgg gccgtgtgca gcgtctgccc gtcgcgcgag atcttcaaca
      121 ccaccgtccg cggcgctccg gcgacggcga cgccgtcgaa gccatgcatg ccgtgcatca
      181 cgacgcagcc ggtccccggg gcgctcacct gctccggcgg ctcgcacgtg gccgacggcc
      241 cgccctcgca gccccgtcgc ggcaggctac cgtcgcacgt caccaccacc ccgtcgaggt
      301 cgaagtcgaa cgtgtacgcg cccgccgccc acggtgatgc tttctcgagc ccgatggaca
      361 gcccgctcat acacccgatc aacgtgcact cccgcggccc cgggttcgcc gtgcagccgg
      421 tcagcagggc gacgaccagg ccgagcaggc gcccgccgcg acctccaagg tcctgcgcgg
      481 cggatcggcg tgctgcgtgc catcgaggtt cgaccacgac gccccagggt accgcggacc
      541 gcccgcgggt gtcatccacg catgatcgcg ccacggggga gcaaaaccgc ggaaacccga
      601 gcactctgga gcgctcgggt cggaaggcca agctggctca ggacccgcgt actgcgaccg
      661 cgtggcgacc tggccggctc gttcgccacc ttcgaggcgc atgtgatcac cttgatcaac
      721 aagaagcggg ccgatcgatc gttgcatcgt cgtggctgtc tccgttgcgg ttcacccccg
      781 aagttccccg cgccatgcgg tctgtctcat ccatcaccca aaaatttatt cgcacctacg
      841 gcagcgggat ccggcggacc caccactgcc gcgcgccgag ctcgaggtga tccttgctgc
      901 ccgcgagcgt caggcagcgc tcgtcggcgg acaggctcgc cgccatcacc gcgccgagcg
      961 cgacgttcgg gctcggctcc atcgtccacg gccaggtccc gtccgcggcg cgcagggccg
     1021 cccacggctt gctgtcgacc gtgccaccga cgatcacgcc gccatccgcc agcggcacga
     1081 tcgtatacgc gaggtcacgc agcagctcgg cccgcagcac atatgcgtcg gcgaacttgc
     1141 cgggcacctt ctcctcccag accggcacgc agttcatcgg gcaggtcgcg ccgagcttcg
     1201 ccagccacag ctgcgtctgc gtcccgtcct gcgcgaagcc tgcgacgtgc accgcgtcca
     1261 tgccggccgg cgccatcgcc atcacgccgg cattcgccag ctcgcacgga cacgcgacgg
     1321 cgccgtccgc cggcccgagc cggcgcagcc acgcgctgta cacgttgtcg cccgcgacat
     1381 tccagctgcc gccgacgatc accgcgtcgg gcagcgcggt gatcgcctgg gcgatcgccg
     1441 ggttgttgaa attcgcgtcg gcgtcgaagg gcaccggctc ggggtcccac gcgggcagcc
     1501 aggccgtccc gtcacgcgcg ccgcgttgat accagatgtt gtcgtcgcgc tcgccggaca
     1561 gcagcagcgt gtccgcgtcc ggcatctcg
//
LOCUS       DASWNJ010000277         6056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_392565, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000277 DASWNJ010000000
VERSION     DASWNJ010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392565"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1249)
                     /locus_tag="VGB85_05005"
     CDS             complement(44..1249)
                     /locus_tag="VGB85_05005"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0133411.1"
                     /translation="MTSSGLDTSRSPIDATEAVARARALAPQVAALAAEAEALRRMPP
                     ALVEAIRAAGLVRMLTPRRFGGHELPFDAFIDATIEIARADASMGWCFSFMNIHSWLL
                     AGFPEQAQAEVWGPDPDVGIANVNMPVGQVTPVPGGYRLSGSWPWASGSDHCDVAILA
                     GIVPAAEGSPPGPPDVRLFVVSRPDFTVQDTWRVAGQRGTGSNNVVVQDAFVPEHRVV
                     PLAELREAQAPGKHVNPGPNYQLPLLPGLAISLVAPIVGAALGAYDRWRDATRTRVTT
                     YSREQVASLSHQQIRMAEVSAELDCAQLLLRRALDTLRSADPLTLEQRVRLRRDYAYI
                     ATLCVRAVERIYLASGAAANYESNPLQRQWRDVHAMVAHTGVNFDSAGENFGRMQLGL
                     PLNPKDPLF"
     gene            complement(1259..2842)
                     /locus_tag="VGB85_05010"
     CDS             complement(1259..2842)
                     /locus_tag="VGB85_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY0133412.1"
                     /translation="MHPRRRPLLLVACCAVGLCVSALFGRSFSDRHSSHAGFHRVEVA
                     DRIDPVFDLRRPRQTALVVFDGLGYVEAQTMRSLSLLQAHGQCRKTHVGPSSMSRPVY
                     AVLSTGLEPDRTGARGNDDTTPMAAQSLWEVARAAGLGVSVVSELPWWRELFPDTFEK
                     YLLAERGENFFRLAPPADLRVIHPLYVDETGHEHGAASSQYQAAVARADRELSEFLAT
                     LDLSRDLVIVTADHGHSLRGGHGGQQDRVAHVLTCHAGVGVRHMPEQRALDTTTIAPS
                     LALLLGLPFPAHMRAGDDDLDALWEIADPDAFPAGYLEDRRAAVERFRGENQATLRSW
                     MPASAGSWDRFHARSRWKQIARALPCLALLIVVFMAQRRLHRVTSPESRHAATFGAGF
                     VVAACLVIYALQVGLRGSFDLSSVAFREEFLRFTVLLGIGWSLGAIGLHLLARRSVTA
                     LIVDWSALSAVGTTLCLAHPLALGWQVGYPAPTPALFFFPYFAALVLGAQNGAGLLLA
                     AIVALRGRSRAYLRPALRE"
     gene            2910..4193
                     /locus_tag="VGB85_05015"
     CDS             2910..4193
                     /locus_tag="VGB85_05015"
                     /inference="COORDINATES: protein motif:HMM:NF024662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4056 domain-containing protein"
                     /protein_id="HEY0133413.1"
                     /translation="MFRPALLLGSLALLGPACAPKWHSATLVDGRAVAKALHDPVGLA
                     MPFDPASVPDFKAPQKVRPCCAFGVDLKAKVGPVPVPLYENENILSPDKIGPHGYDKG
                     ELTKENNGLVYTCRGGFIDLAHVRDTGDNTLFLAMQIARGLPGEITIQMPDQGTKRRI
                     TIKAVPEALLARYGRWTVAAALASYVAYDLSIWHEVVTWYGWESTMGISEKLSAFSPE
                     DTYSNVLGINLVAGIVLEREASSREQYDQSMEVWIREAMRRLGAVKEEQGRKAMKAVD
                     GLWWDSNKRLPDWKLVTRRDLHITSPVPPWIVEDSLPADKLTPEVKQMCQGQPPPLPL
                     EIEDRIGNTKIEDLVAIQFEFDEWIPKDFPLPADKGQSVQRTDIPKIVEDIRQKAAQD
                     LGAGFDQPKVAPGDAKPAPAETKAEPAEAKAGPAS"
     gene            4237..>6056
                     /locus_tag="VGB85_05020"
     CDS             4237..>6056
                     /locus_tag="VGB85_05020"
                     /inference="COORDINATES: protein motif:HMM:NF042814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEY0133414.1"
                     /translation="MPEPRDDAPCGTQALALFTALADRLHARGEAPQAVARLRDRLIV
                     CLFAAHAGALDRAVLERALAGIPDACAAMIREIGIFADASDVSLDPDELGLLRAAASL
                     DWSSIEPTILGTLFECGLDPVRRSRLGAHYTDREAISKIIEPVVMTPLRREFAEVQAL
                     TGSRNEVAERYQAYLARLRGVRVLDPACGAGHFLYHALVALQDLEKEVLAWGARRLDM
                     PIRPALAFEVVLGIERDTEAAQLARVTLWIAELRRGRRAPALPATIACRDAILELSPT
                     GQPLRPVWPDAEFIVGNPPFLGGKKLRAELGDHYVELLFAAWAGRVPAEADLVSYWHE
                     AAREQIAAGRCRRAALLATQGIRGGANLEVLRRIKASGDIFMAWRDQPWTTQGAAVRV
                     SMVAQDDGSEQQRSLDGVAVAVIHADLTGGVAHTPDVTRARRLRENAGVAFMGDTKGG
                     KFELSPADAASMLRAAPANREVVVPWVNGLDVTRRPRGMHIVDFGVDMPCATAARYTA
                     PFAYVQDRVAPARAVNRRDAYRERWWIHVEPRPALRTAIRPLRRFIATPTVARSRVFV
                     WLTPPTLPDHQLIVVARDDDYCMGVLHSHIHELWSLRMCT"
BASE COUNT          956 a         2050 c         2220 g          830 t
ORIGIN      
        1 ccgcatgcag ccgccggtca ccgccgcgcc catgggccgg cgatcaaaat agcgggtcct
       61 tggggttgag cggcaggccg agctgcatgc ggccgaagtt ctcgcccgcg gagtcgaagt
      121 tgacgccggt gtgcgcgacc atcgcgtgca cgtcgcgcca ctggcgttgc agcgggttgc
      181 tctcgtagtt cgccgctgcg ccgctggcga gatagatgcg ctcgacggcc cggacgcaca
      241 gggtcgcgat atatgcgtag tcgcgacgca ggcgcacgcg ctgctcgagc gtcagggggt
      301 cggcggagcg cagggtgtcg agggcgcggc gcagtagcag ctgcgcgcaa tccagctcgg
      361 ccgagacctc ggccatgcgg atctgctggt gcgacaggga cgcgacctgc tcgcggctgt
      421 aggtggtgac gcgggtgcgg gtcgcgtcac gccagcggtc gtaggccccg agcgcggcgc
      481 cgacgatcgg cgcgacgagc gagatcgcca ggcccgggag cagcggcagc tgatagttcg
      541 ggcccgggtt gacgtgcttg cccggcgcct gggcctcgcg cagctcggcg agcgggacca
      601 cgcgatgctc cggcacgaag gcgtcctgca cgacgacgtt gttgctgccg gtgccgcgct
      661 ggccggcgac gcgccaggtg tcctggacgg tgaagtcggg gcgcgagacc acgaacaggc
      721 gcacgtccgg cgggcccggc gggctaccct cggccgcggg cacgatgccg gcgaggatcg
      781 cgacgtcgca gtggtcgctg ccgctggccc acggccacga gccggacagg cgataaccgc
      841 cgggcacggg cgtcacctgc ccgaccggca tgttgacgtt ggcgatcccg acatccggat
      901 cagggcccca cacctccgcc tgcgcctgct cggggaagcc ggcgagcagc cacgagtgga
      961 tgttcatgaa cgagaagcac cagcccatcg atgcatcggc gcgggcgatc tcgatcgtcg
     1021 cgtcgatgaa ggcgtcgaag ggcagctcgt ggccgccgaa gcgacgcggc gtcaacatgc
     1081 gcaccagccc cgcggcgcgg atcgcctcga ccagcgccgg cggcatgcga cgcagcgcct
     1141 cggcctcggc cgcgagcgcg gccacctgcg gcgcgagggc ccgcgcccgt gcgaccgcct
     1201 cggtcgcgtc gatgggagat cgtgaagtgt cgagcccgga tgaggtcatc cgccgatact
     1261 actcgcggag cgctggccgc agatatgccc ggcttcgccc gcgcagcgcg acgatcgccg
     1321 cgagcagcag gccggcgcca ttttgcgcgc cgagcaccag cgccgcgaag tacgggaaga
     1381 agaacagcgc gggggtcggc gccgggtagc cgacctgcca gccgagcgcg agcgggtgcg
     1441 cgaggcacag ggtcgtgccg acggccgaca gcgcgctcca gtcgacgatc agcgccgtga
     1501 cgctgcgccg cgcgagcagg tgcaggccga tcgcgccgag gctccagccg atccccagga
     1561 ggacggtgaa gcgcaggaac tcctcgcgaa aagcgaccga gctgaggtcg aagctgccgc
     1621 gtagcccgac ctgcaatgca tatatgacga ggcaagcggc gacgacgaag ccggcgccga
     1681 aggtcgcggc gtgccgtgac tccggcgagg tcacgcgatg caaccgacgc tgcgccatga
     1741 atacgacgat cagcagggcg aggcagggca gggcgcgggc gatctgcttc cagcgcgaac
     1801 gggcgtggaa gcggtcccag ctgcccgcgc tcgccggcat ccagctgcgc agggtggcct
     1861 ggttctcgcc gcgaaagcgc tcgacggcgg cgcgccggtc ctcgaggtaa ccggccggga
     1921 acgcatccgg gtcggcgatc tcccacaatg catcgaggtc gtcgtcgccg gcccgcatat
     1981 gcgccgggaa cggcaggccg agcagcagcg cgagcgacgg ggcgatcgtg gtggtatcga
     2041 gcgccctctg ctccggcata tgacgcacgc cgacgcccgc gtggcaggtg agcacgtgcg
     2101 cgacgcggtc ctgctggccc ccgtggccgc cgcgcagcga gtggccgtga tccgcggtga
     2161 cgatcaccag gtcgcgcgag agatcgaggg tggcgaggaa ctcggacagc tcgcggtcgg
     2221 cccgcgcgac cgcggcctga tattgcgacg acgcagcgcc gtgctcgtgc cccgtctcat
     2281 ccacatacaa cggatggatc acgcgcaggt ccgcaggcgg tgcaaggcga aagaaattct
     2341 caccccgctc cgccagcaga tacttttcaa atgtgtcggg gaagagctcg cgccaccatg
     2401 gcagctcgct gaccaccgat acgccgaggc ccgccgcgcg cgcgacctcc catagagatt
     2461 gcgcggccat cggcgtggtg tcgtcgttgc cacgcgcgcc ggtccggtcc ggctcgagcc
     2521 cggtcgacag cacggcgtag accgggcgtg acatcgacga cgggccgacg tgggtcttgc
     2581 ggcactggcc gtgcgcctgc aggagcgaaa gcgatcgcat cgtctgcgcc tcgacgtagc
     2641 cgaggccgtc gaacacgacg agcgcggtct ggcgcgggcg gcgcaggtcg aacacggggt
     2701 cgatgcggtc ggcgacctcg acgcgatgaa agcccgcgtg tgacgagtgg cgatcggaga
     2761 agctgcggcc gaacagcgcc gacacgcaca ggccgaccgc gcagcaggcg acgaggagca
     2821 gcggtcggcg ccgggggtgc atcgggccct cagcatggat ccggtcgcgg cgctgcgcag
     2881 cgcgggaagt gctgctacga gtatcgccca tgtttcgtcc tgcgctgctg ctcggatcgc
     2941 tcgctctcct cggccctgcg tgtgcgccga agtggcactc tgccacgctg gtcgacggtc
     3001 gagcggtggc gaaggccctg cacgatccgg tcggcctggc gatgcccttc gatccggcct
     3061 cggtccccga cttcaaggcc ccgcagaagg tccggccctg ctgcgcgttc ggcgtcgatc
     3121 tcaaggccaa ggtcggcccg gtgccggtgc cgctgtacga gaacgagaac atcctctcgc
     3181 cggacaagat cgggccgcac ggctacgaca agggcgagct gaccaaggag aacaacggcc
     3241 tcgtctacac ctgccgcggc ggcttcatcg acctcgcgca cgtgcgcgac acgggggaca
     3301 atacgctctt tctggcgatg cagatcgcgc gcggcctgcc gggcgagatc acgatccaga
     3361 tgccggatca gggcacgaag cgacggatca cgatcaaggc ggtgcccgag gcgttgctcg
     3421 cacggtacgg gcgctggacg gtcgcggcgg cgctcgccag ctatgtcgcc tacgacctct
     3481 cgatctggca cgaagtagtc acctggtacg gctgggagag caccatgggc atctcggaga
     3541 agctgtccgc gttctcgccc gaggacacat actccaacgt gctcggcatc aacctcgtgg
     3601 ccggcatcgt gctcgagcgc gaggcgagct cgcgcgagca gtacgatcaa agcatggagg
     3661 tgtggatccg cgaggcgatg cgccgcctcg gcgccgtcaa ggaggagcaa ggccgcaagg
     3721 cgatgaaggc cgtcgacggg ctgtggtggg actcgaacaa gcggctgccc gactggaagc
     3781 tcgtcacccg ccgcgacttg cacattacga gccccgtgcc gccgtggatc gtcgaggatt
     3841 cactgccggc cgacaagctc acccccgagg tcaagcagat gtgccagggc cagccgccgc
     3901 cgctcccgct cgagatcgag gacaggatcg gcaacaccaa gatcgaggac ctcgtggcga
     3961 tccagttcga gttcgacgag tggatcccga aagacttccc gctgccggcg gacaaaggcc
     4021 agtcggtcca gcgcaccgac atcccgaaga tcgtcgagga catccggcaa aaggccgcgc
     4081 aggacctcgg cgccggcttc gaccagccga aggtcgcccc cggcgatgcg aagcccgccc
     4141 cggccgagac gaaggccgaa ccggccgagg cgaaggccgg gccggcgtcg tgaccgcggc
     4201 tgccggcgcg cgaggccgct gatatgctcg cccgcggtgc ccgagccccg agatgacgcg
     4261 ccctgtggga cccaggcgct cgcgctgttc accgcgctcg ccgaccggct ccacgcgcga
     4321 ggagaggcgc cgcaggcggt cgcgcggctg cgcgaccgcc tgatcgtctg cctgttcgcg
     4381 gcgcacgcgg gcgcgctcga ccgggcggtg ctcgagcgcg cgttggctgg catacccgat
     4441 gcatgcgccg cgatgatccg tgagatcggt atatttgcgg atgcaagcga cgtatcactc
     4501 gatcccgacg agctgggcct gctgcgagcc gcggcgtcgc tcgactggtc gagcatcgag
     4561 ccgacgatcc tcgggacact gttcgaatgt ggcctcgatc cggtgcggcg cagccggctc
     4621 ggcgcgcatt acaccgaccg tgaggcaatt tcaaagatca tcgagccggt ggtgatgacg
     4681 ccgctgcgcc gcgaattcgc cgaggtacag gcgctcaccg gttcgcggaa cgaggtggcg
     4741 gagcggtatc aggcgtatct tgcgaggcta cgcggcgtgc gggtgctcga ccccgcgtgc
     4801 ggcgcgggac actttttata ccacgcactt gtcgcgttgc aggaccttga aaaagaggtg
     4861 ctcgcctggg gcgcgcggcg gctcgatatg ccgatacgac cggcgctcgc cttcgaagtg
     4921 gtcctcggga tcgagcggga cactgaggcc gcgcagctcg cccgtgtcac gctctggatc
     4981 gcggagctgc ggcgcggtcg acgggcaccc gcgctgccgg caacgatcgc ctgccgggac
     5041 gcgatcctcg agctttcgcc caccgggcag ccgttgcggc cggtctggcc ggacgcggaa
     5101 ttcatcgtcg gtaatccgcc gttcctcggc ggcaagaagc tgcgcgccga gctcggcgac
     5161 cattatgtcg agctgttgtt tgcggcgtgg gccggcaggg tcccggcgga ggcggatctg
     5221 gtcagctact ggcacgaggc cgcgcgcgag cagatcgcgg ccggtcgctg ccggcgggcc
     5281 gcgctgctgg ccacgcaggg gatccgcggc ggcgcgaacc tcgaggtgct gcgccggatc
     5341 aaagcgagcg gcgacatctt catggcctgg cgcgatcagc cgtggacgac ccagggggcg
     5401 gccgtgcgcg tgtcgatggt cgcgcaggac gacggcagcg agcagcagcg gagcctcgac
     5461 ggcgtcgcgg tcgcggtcat ccacgcggac ctgacgggag gcgtcgcgca cacgccggac
     5521 gtgacgcggg cgcggcggct gcgagaaaac gccggcgtcg cgttcatggg cgacaccaag
     5581 ggcggcaagt tcgagctctc gcccgcggac gcggcgtcga tgttgcgggc cgcgcccgcc
     5641 aaccgcgagg ttgtggtccc gtgggtcaac gggctcgacg tcacgcgccg cccgcgtggc
     5701 atgcatatcg tcgacttcgg ggtcgacatg ccgtgcgcga ccgcggcgcg gtacacggcg
     5761 ccgttcgcat atgtgcagga ccgggtcgcg ccggcgcggg cggtcaatcg ccgcgatgca
     5821 taccgcgagc gctggtggat ccacgtcgag ccgcggccgg ccctgcggac tgcgatccgg
     5881 ccgctgcggc gcttcatcgc cacgccgacg gtcgcccgga gccgggtgtt cgtgtggctg
     5941 accccgccga cgctgccgga tcaccagctg atcgtcgtcg cccgcgacga cgactattgc
     6001 atgggcgtgt tgcactcgca tatccacgag ctgtggtccc tgcgcatgtg cacctg
//
LOCUS       DASWNJ010000278         1921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_111289, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000278 DASWNJ010000000
VERSION     DASWNJ010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111289"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VGB85_05025"
     CDS             complement(<1..364)
                     /locus_tag="VGB85_05025"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY0133415.1"
                     /translation="MVIMIPGDKDVEAGKMPDEQAIAAMMEFNQELVKAGVLLAGEGL
                     HPSSKGARIRFAGGKPSVTDGPFSEAREIIGGFWIWQVASRDEAIAWASRCPAGEHDT
                     LEIRQVFEPEEFGPEVAAK"
     gene            complement(494..1327)
                     /locus_tag="VGB85_05030"
     CDS             complement(494..1327)
                     /locus_tag="VGB85_05030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0133416.1"
                     /translation="MSDYKDIARNVFNSLDIVAWATDAEGVILLMEGKALQPMGMTPG
                     QFIGVNVLELYRDNPVTSAALKRALAGEPHVSHTFEAGRTVETQFTPMFDAAGALIGV
                     AGLSIDVTERLAAQAEIERQTEALRRQAEQLAQAELAEARRQETIRAQALAIQELSTP
                     LIPITDEILVMPLIGTMDSVRAKQVLQAILEGAAACRARWVILDITGVAVVDTAVANT
                     ILNAGQAARLLGSEVIVTGIRPEVAQTLVQLGADLGHITTRGTLQSGIAHALGKRSLS
                     R"
BASE COUNT          280 a          607 c          723 g          311 t
ORIGIN      
        1 ccttggcggc gacctcgggg ccgaattcct cgggctcgaa gacctgacgg atctcgaggg
       61 tgtcatgctc gccggcgggg cagcgcgagg cccacgcgat cgcctcgtcg cgcgaggcga
      121 cctgccagat ccagaagccg ccgatgatct cgcgggcctc actgaagggc ccatcggtga
      181 cgctcggctt gccgccggcg aagcggatcc gcgcgccctt cgagctcggg tgcaggccct
      241 cgcccgcgag cagcacgccg gccttcacca gctcctggtt gaactccatc atcgcggcga
      301 tcgcctgctc gtccggcatc ttgccggcct caacgtcctt gtcgcccggg atcattatca
      361 ccatgaatcg catcgtcgtt ctccgtgtgg gggggttgta tctccccgtc gaacgaggcg
      421 cagccggatc gacaacgcga cgcgcaattt tttgagcgcg ggataatatg cggtgcgcca
      481 ccggcgctgg gcctcaacgc gagaggctgc gtttgccgag cgcgtgggcg atgccgcttt
      541 ggagggtgcc gcgggtggtg atgtggccga ggtcggcgcc gagctggacg agggtctggg
      601 cgacctccgg gcggatgccg gtgacgatga cctcgctgcc gagcaggcgg gcggcctgac
      661 cggcgttgag gatcgtgttg gcgacggcgg tgtcgacgac ggcgacgccg gtgatgtcga
      721 ggatcaccca gcgcgcgcgg caggcggcgg cgccctcgag gatcgcctgc agcacctgct
      781 tggcgcggac cgagtccatg gtgccgatca gcggcatgac gaggatctcg tcggtgatcg
      841 ggatcagggg ggtcgagagc tcctggatcg ccagcgcctg ggcgcggatc gtctcctggc
      901 ggcgcgcctc ggcgagctcg gcctgcgcga gctgctcggc ctggcggcgc agcgcctcgg
      961 tctgacgctc gatctcggcc tgggcggcca accgctcggt gacgtcgatc gagagcccgg
     1021 cgacgccgat gagcgcgccc gcggcgtcga acatcggcgt gaactgggtc tccacggtgc
     1081 gcccggcctc gaaggtgtgt gagacgtgcg gctcaccggc gagggcccgc ttgagcgcgg
     1141 ccgaggtgac cggattatcg cggtacagct cgagcacgtt gacgccgatg aattgccccg
     1201 gcgtcatgcc catcggctgc aatgccttgc cttccatcaa cagaatgacg ccctcggcgt
     1261 cggtcgccca ggcgacgata tcgaggctgt tgaagacatt ccgggcgata tctttgtagt
     1321 cgctcatgga tatgctcccg gtcgtgagga agcgacgatc cggcagctgc caaacccgtc
     1381 gcgccgggcc cagaacaccc tgaacgctgt gtcccggatc ggcaagccgt attttgggtc
     1441 gcgccgaagg gatattcgcg gccgcggact acgtgcatat ctgtcgccgg acttggccgg
     1501 tttgccggcg gatatgcgcg aggttgcccg cgcagcacag ggcgcaagcg tgggctcggg
     1561 cttccccgca agtgaaagcc cggagcagag ccggggccga cgtcagaacg aggcggacag
     1621 cgaggggccg ccgaatgtcg gtagtatgcg cacccgggtg tgatcgcggc ggtcgtggag
     1681 atgcctgccg aggccggcga gcacgagcga ggtgatcacg acgcgaagga cggtgacgcc
     1741 ctcgcggcgg atgtcggttc gggcgtcgcg caggccggcg ctgggctcaa cgcgagaggc
     1801 tgcggttgcc gagcgcgtgg gcgatgccgc tctggagggt gccgcgggtg gtgatgtggc
     1861 cgaggtcggc gccgagctgg acgagggtct gggcgacctc cgggcggatg ccggtgacga
     1921 t
//
LOCUS       DASWNJ010000279         1657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_41012, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000279 DASWNJ010000000
VERSION     DASWNJ010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41012"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..>1657
                     /locus_tag="VGB85_05035"
     CDS             95..>1657
                     /locus_tag="VGB85_05035"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEY0133417.1"
                     /translation="MTDASLHSLLDMGLPADTERAVRRWLEDPHYASERPALGALLAE
                     VEGGSAAARAELLDAFSQVLPIGTGGRRGRVGPGPNRMNPVVVRETAQGVVEALKAAG
                     DTPQVVIVYDTRMHSRQFAYAAAEQCAAGGLTVVLLDAPRPTPELSFLVRHTGAGAGI
                     VVSASHNPPEDNGIKIYGPDGAQVLGARDAALMRAILGVTQLPKLTDDQRSQIHVIGP
                     EQVEGEADKPYHNYVLAQGVVPGSLEARGFSLVFTPLHGVGHTSAAAVLKARGLTVHL
                     VAAQCDADGGRFSTVASANPELPVSMAMAVELAEAEGAQLVLATDPDADRLGACARGR
                     DGTFVAIDGNRLGALMLDHVLDKMAGRLPPNGWVLTTLVTTPLIATLARAHGVEVVDD
                     LLVGFKHHAGMIGEHPEKTLVFGCEESHGFVRGDDIRDKDGAIAALLLAECAAVAHAE
                     GHTLFDNLERIWCRHGYHREKTANLVAPGLAGRRAITAVMQTWRTRPPEGFAGLQVVR
                     VDDRMALRNTGSA"
BASE COUNT          241 a          580 c          590 g          246 t
ORIGIN      
        1 ccggaagcgc ttcagtgttc catggaacga gccccgcgag gagatgattt caccgcgcag
       61 caagttcctc gcagtttgag gtaggatttc gcccgtgacc gacgcatccc ttcacagcct
      121 cctcgatatg ggtctgccag ccgacaccga gcgtgccgtt cgccgttggc tcgaagatcc
      181 gcactatgcc tccgagcgcc ccgcgctcgg cgctttgctc gccgaggtcg aggggggctc
      241 ggcggccgcc cgcgccgagc tgcttgatgc cttctcgcag gtcctgccga tcggcaccgg
      301 aggccggcgt gggcgcgtcg ggcccgggcc caaccgcatg aacccggtgg tcgtgcgcga
      361 gacggcccag ggcgtggtcg aggcgctcaa ggccgccggg gacacgcccc aggtcgtcat
      421 cgtgtacgac acgcggatgc actcgcggca gttcgcgtac gcggccgccg agcagtgtgc
      481 ggccggcggg ctgacggtcg tgctgctcga cgcgccgcgg ccgacgcccg agctgtcgtt
      541 cctggtccgg cacacggggg ccggcgcggg gatcgtcgtc agcgcgagtc acaacccgcc
      601 cgaggacaac gggatcaaga tctacgggcc cgacggcgcg caggtgctcg gcgctcgcga
      661 cgcggcgctg atgcgggcga tcctcggggt gacgcagctg ccgaagctga ccgacgacca
      721 gcgctcgcag atccacgtca tcggacccga gcaggtcgag ggggaggccg acaagcccta
      781 ccataattac gtactggccc agggggtcgt gccgggctcg ctcgaggccc gcgggttctc
      841 gctggtgttt accccgttgc acggggtcgg ccacaccagc gcggcggcgg tcctcaaggc
      901 ccgcggcctg acggtgcatt tggtggcggc gcaatgtgat gccgacggcg gccgattctc
      961 gacggtcgcc tcggccaacc cggagctgcc ggtgtcgatg gcgatggcgg tcgagctcgc
     1021 cgaggccgag ggcgcccagc tggtcctcgc caccgacccc gacgccgacc gcctgggcgc
     1081 atgcgcgcgc ggccgcgacg gcaccttcgt ggccatcgac ggcaaccgcc tcggcgccct
     1141 gatgctcgac cacgtgctcg acaagatggc cggtcgcctc ccgcccaatg gctgggtgct
     1201 gacgacgctg gtgacgactc cgctgatcgc taccctcgca cgggcgcacg gagtcgaggt
     1261 cgtcgacgat ctgctcgtgg gcttcaagca tcacgccggc atgatcggcg aacaccccga
     1321 gaagactttg gttttcggat gcgaggagag ccacggcttt gtccgcggcg acgacatccg
     1381 cgacaaggac ggagcgatcg ccgcgctgct gcttgcagag tgcgccgcgg tggcccacgc
     1441 cgagggacac accctgttcg acaacctcga gaggatctgg tgccgtcacg gctatcaccg
     1501 cgagaagact gcgaatctcg tggcgcccgg gctcgccggt cggcgcgcga tcacggcggt
     1561 gatgcagacc tggcggaccc gcccgcccga gggcttcgcg ggtctgcagg tcgtccgcgt
     1621 cgacgaccgc atggccctgc gcaacaccgg gtcggcg
//
LOCUS       DASWNJ010000280         3162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_287992, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000280 DASWNJ010000000
VERSION     DASWNJ010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287992"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VGB85_05040"
     CDS             complement(<1..298)
                     /locus_tag="VGB85_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133418.1"
                     /translation="MVSDGTVSIKVFCQPCRLKVGDPLSQPLLAFETGGVQKTSDTVP
                     SACATNDRFAHECRGIVTSADLAMICIGEFNVALDVPLPGDVNVGDWVCFECARI"
     gene            complement(288..890)
                     /locus_tag="VGB85_05045"
     CDS             complement(288..890)
                     /locus_tag="VGB85_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133419.1"
                     /translation="MGVAGCIARNLAQDYLTVKAVEAGTALLGPLLGASEVPPPPRKP
                     TAAVVSTKQIKHTSFWAALAGAIAGAIAAAAISALFVAAVGATGGAAIAVGVAACFVY
                     GSVISDISSGVTDFVDGLFPADDGPVLPASTTVTIGGLPAARAGVESTGHPVRSQPFT
                     SRAFVNSPGKHLAWGGFYDTNRLARQLDVCLRRRGSPHGF"
     gene            complement(892..1335)
                     /locus_tag="VGB85_05050"
     CDS             complement(892..1335)
                     /locus_tag="VGB85_05050"
                     /inference="COORDINATES: protein motif:HMM:NF020366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1795 domain-containing protein"
                     /protein_id="HEY0133420.1"
                     /translation="MRSLHGVMAYTFNEGNLVIPDDWRDESMNIFKAPVEAGYNLVLS
                     RERIPRSLEPRMHLEAQRKVIEENLLGFREHERRSLMLDGQECVWLDYSWQSPEGPMH
                     QINVMRTVVDTLLSFTFTSAQRITPAQRDLFRRILESYSEPRQAA"
     gene            1509..2093
                     /locus_tag="VGB85_05055"
     CDS             1509..2093
                     /locus_tag="VGB85_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133421.1"
                     /translation="MFEVRPPRLPTGCTAIVDPALGVVVGYRYDSGGYWAIYDVTGEL
                     VESGELPLESPLLDPIDILARGLVGGLRAGLRGLAGGGGRGLLVGGARGAARALSTRA
                     WMALHRGVRPFLRPNSLKFAATPAAHMLEPWRHVPVHILHLAIRHGRQVPDPKRAAGA
                     VKYIAQMWRNGKPYTLEVVVRVRDSMIYHFEYYR"
     gene            2101..2619
                     /locus_tag="VGB85_05060"
     CDS             2101..2619
                     /locus_tag="VGB85_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133422.1"
                     /translation="MSTTLPTLLLTRRDRPCRASCSYDATSAVFSATFAYAGPPPPAD
                     LRRRIDDSLGIREPHARLRVGDVDIVLVERRLDSLVIRTSPEQWIRRPLGAPDVADPV
                     WFEPVCAFDANLIAEVSTTTTYARDGAALDVRFAGAGVVRWVELADAVFAGLTVDARL
                     CGLLFARCPLPP"
     gene            complement(2873..>3162)
                     /locus_tag="VGB85_05065"
     CDS             complement(2873..>3162)
                     /locus_tag="VGB85_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133423.1"
                     /translation="AGLPYPARRELLEWHHLVGAQSRAWSALSEAERELALADSPIAS
                     GLPAPVTELIELEARLGDVLTTLHHQELARVQAALREVDAWLRARPARADA"
BASE COUNT          487 a         1097 c         1056 g          522 t
ORIGIN      
        1 caattcgagc acattcaaag catacccaat cccccacatt gacatcgccc ggaagaggca
       61 catcaagcgc gacgttaaat tccccaatgc agatcattgc aagatccgcg ctcgtcacaa
      121 taccgcgaca ctcgtgtgca aaacgatcat tggtggcaca cgcactcgga acggtgtccg
      181 atgttttttg cacacccccc gtttcgaacg cgagaagcgg ttgagaaagc ggatcgccca
      241 ccttcaaccg gcatggttga caaaacacct taatggacac cgtgccgtca gaaaccatga
      301 ggactgcctc ttctgcggag acagacatcc aattgacgcg caagacggtt ggtatcatag
      361 aatccacccc aggctaggtg ctttcctggc gagttcacga atgctcttga cgtgaaaggt
      421 tgtgagcgca ccggatgccc ggttgattcc acgccggcgc gcgcggccgg caggccgccg
      481 atcgtcacgg tcgtggacgc gggcagcacg ggtccgtcgt cggcgggaaa cagcccgtcg
      541 acgaagtcgg tgaccccgga ggatatgtcg ctgatcaccg acccatagac gaagcaggca
      601 gccacgccga cggcgatcgc cgcgccgccg gtcgcgccaa cggcggccac gaagagggcg
      661 gagatcgccg ccgccgcgat cgccccggcg atggcgcccg cgagcgccgc ccagaagctc
      721 gtatgtttga tctgcttggt cgagacgacc gcggcggtcg gcttacgcgg cgggggcggc
      781 acctccgagg cgccgagcag cgggccgagc agcgccgtcc cggcctcgac ggccttgacg
      841 gtcaggtagt cctgggccag gttccgcgcg atgcagccgg cgacgcccat gtcaggccgc
      901 ctgcctcggc tcgctgtagc tctcgaggat ccggcggaac aggtcgcgct gggcgggtgt
      961 gatgcgctgc gccgacgtga acgtgaagct cagcagggta tcgaccacgg tccgcatgac
     1021 gttgatctga tgcatcggac cctcggggga ttgccacgag tagtcgagcc acacgcactc
     1081 ctgaccgtcg agcatcaggc tgcggcgctc gtgctcgcgg aagcccagca ggttctcctc
     1141 gatcaccttg cgctgggcct cgaggtgcat ccgcggctcg agcgaccgcg ggatgcgttc
     1201 acggctgagc acgaggttgt agccggcctc gaccggcgcc ttgaagatgt tcatcgactc
     1261 atcgcgccag tcgtcgggga tgaccaggtt gccctcattg aacgtgtacg ccatgacacc
     1321 gtgcaggcta cgcacgtctc ttcgcctgcg acagccgaag aattgttgct gcgatgcaac
     1381 cggagctacc ctgctgagct tggggttgcg gcctgacgcc acggctgcgc atggtttctc
     1441 gcgtggaacc gcaaatacat gatgagggcg acgacggtgt cgtcgtccaa ccgccgcgga
     1501 ctgctgacgt gttcgaggtc cgtccgccga ggctccccac ggggtgcacc gcgatcgtcg
     1561 atccggcgct cggcgtcgtc gttggttacc gctatgactc gggcggctac tgggcgatct
     1621 acgatgtcac cggggagctc gtcgagagcg gcgagctccc gctcgagtca ccgctcttgg
     1681 atccgatcga catcctcgcg cgtggactcg tcggagggct gcgcgcgggt ctccgcgggc
     1741 tcgccggtgg tggcggccgt gggctcctcg tcggcggcgc gcgaggggcc gcccgggcgc
     1801 tgtccacccg ggcctggatg gcgctgcacc gcggggtccg cccgttcctt cgcccgaaca
     1861 gcttgaagtt cgcggcgacg cccgccgccc acatgctcga gccctggcgc cacgtcccgg
     1921 tccacatcct ccacctggcg atccgacacg gcagacaggt ccccgatccg aagcgcgcgg
     1981 cgggtgccgt caaatacatc gcgcagatgt ggcgcaacgg caaaccgtat accctggagg
     2041 tcgtggtccg cgtccgcgac tcgatgatct accacttcga atattatcgt tgaggcagac
     2101 atgtcgacga ctctccccac gctcttgctc acccgacgcg accggccctg ccgtgcgagc
     2161 tgcagctacg acgcgacgtc ggcggtgttc tcggccacgt tcgcctacgc cgggccgccg
     2221 ccgcccgcgg acctccggcg gcgcatcgac gactccctcg ggatccgcga accccacgcc
     2281 cgcctgcgcg tcggcgacgt cgacatcgtc ctggtcgagc ggcgcctcga ttcgctcgtc
     2341 atacggacgt cgccggagca gtggatccgc cgcccgcttg gtgcgccgga cgtggccgat
     2401 ccggtgtggt tcgagcccgt ctgtgcgttc gacgccaacc tcatcgccga ggtctccacg
     2461 accaccacgt acgcacgcga cggcgcggcg ctcgacgtgc gattcgccgg cgcgggggtc
     2521 gtccgctggg tcgagctcgc cgacgctgtg ttcgccggct tgaccgtgga cgctcgcctg
     2581 tgcggcctgt tgttcgcccg ctgtccgctc ccgccctgag gcccgccgcg ggcctactcg
     2641 agcgcgatcg agcagtttct cgccgcagcc cgtgtcccat cgggtgcgtc ttcgggtttc
     2701 agcagcggac cgtgacggct gttagggaag ggcggggtcg tggactcgag gcgaccggcc
     2761 ggctggcggc aggtttgcct tcagacccat ccaagccccg tttgcgcggc tctgagatct
     2821 cctgtccgtg gccgccaggc gcgtccggct gtcaaagcgc gatgcggtgc gctcacgcgt
     2881 ccgcgcgggc gggccgagca cgcagccacg cgtcgacctc gcgcagcgcg gcctgcaccc
     2941 tcgccagctc ctgatgatgg agggtggtga gcacgtcgcc gagccgcgcc tcgagctcga
     3001 tgagctccgt caccggcgcc ggtagccccg aggcgatcgg ggagtccgcg agcgccagct
     3061 cgcgctcggc ctccgacagc gcggaccagg cccgggactg cgcgccgacg aggtggtgcc
     3121 actcgagcag ctcgcgccgg gccgggtatg gcaggccggc gc
//
LOCUS       DASWNJ010000281         5123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_111307, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000281 DASWNJ010000000
VERSION     DASWNJ010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111307"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VGB85_05070"
     CDS             <1..837
                     /locus_tag="VGB85_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133424.1"
                     /translation="RGGGPLSLRARSGPGEEPDKRWSEWKTVTLAKQGDALRGALAVP
                     GRRYVQLEVGLDAVSELRDISLFYAPENLAPLVQSVDIAGPEIDDEDEGEPDPKLTIK
                     WKAEARDDDELVYEVRIRPEGSDATQWIKLGGENPVTKKELKWDLGSVPDGVYEAQIV
                     ASDEPSNGSARARTDEVVSAPFIVDHKRPTIDEVKVQGARIVARARDEGSRILGVSYS
                     IDGGAFRAASPADGLFDGDSETFEVTLPADLKPGKHRVVLRARDAFGNLGTVAVVATT
                     DR"
     gene            985..2985
                     /locus_tag="VGB85_05075"
     CDS             985..2985
                     /locus_tag="VGB85_05075"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0133425.1"
                     /translation="MNNRIAPGIILGIFTSSVAHAEPWVEDKAFFTETHQSGSSMVDA
                     ADLDGDGWIDLVFANGGGYNKGSLDNDLKQQAFHNEAGAAMTPIESPDIFGGVTYTGR
                     AVKIRDIDNDGDNDIFLGVTWQGQSQLFVNDGAGNFVNETAINLPAINASVGDLEFGD
                     IDDDGDLDVMLADWGPDSPVDDPINPGGVTRLWLQDGAPGHFGPGTAMFSDFTVDQMP
                     DIGIRWSWDLEFVDLDNDYDLDAVVSCFACTGSSVHLFANDSAGTFTAVPLANAPQGA
                     GALSVEAIDLDGDAFLDLVTTHDAAGGRNRVLLSDGLGAFSDASLVHWPASENPASYD
                     HMAAFLDHDSDGRPDIVLGALQPGLNKYPDRLMVNQNGKFTANNAAFAEIKASSGTYA
                     IVLADFNRDHRLDVAMAQNENAFDKKVFLASDVERTPDTLAPRFINHEALPADLEFGQ
                     DHTLRLRCHDNKSPLMLHDFQRGVDDMPDGRPFVESWNTTPADPDTTPPDETSAPGQW
                     YGEYLWRVRFSVPKQAALFAYRICAIDAAGNKGCTELASVTNQDAGTTGDLDTTTGTA
                     GSSSSTSDTGSTSSAGTTTTDPAPTGTTPTGTTGPSDTPTTGGQDDGSAPMTTTAASS
                     SSSDTAPHDPGTDGGCSCNGRPGPRSGLFALLLVLCTRRRRA"
     gene            3042..3536
                     /locus_tag="VGB85_05080"
     CDS             3042..3536
                     /locus_tag="VGB85_05080"
                     /inference="COORDINATES: protein motif:HMM:NF019462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133426.1"
                     /translation="MSIAAELAASPPSALAARMARLRWSDFGLDLASQLPRRLWSSHK
                     LHFDRACEIGLFFLPRGERIPLHDHPHIHVWMRVLCGRLRATSFTWAARPLARRSGDA
                     VLDAGSPVWAVLPEQDNLHELEALEDVAFLDVLRPPYIDGRVCTYYAAAPAHDDLWHM
                     TALP"
     gene            complement(3547..4104)
                     /locus_tag="VGB85_05085"
     CDS             complement(3547..4104)
                     /locus_tag="VGB85_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133427.1"
                     /translation="MPTRLPDFPLVSAGPATVGCRGRGMTSYHEVALWALQLPYGRGA
                     DRDDLAILHEGRGTCSTKHALLARLAREHGVDVELVLGIYLMDGHNTPGVGSVLAAHG
                     LTAIPEAHCVLRWNGQYIDLTRAGAREPSFIHEERIRPEDIVTHKVAIHRRFLAQWMT
                     TNLPGWTLDSVWRVREACIAALAGT"
     gene            complement(4143..4703)
                     /locus_tag="VGB85_05090"
     CDS             complement(4143..4703)
                     /locus_tag="VGB85_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEY0133428.1"
                     /translation="MTSLRNYVLPLIVAMTAFQAGCGGDADSESATAELQALGDSNVT
                     GKATFTATSDGKVLVEADISGLTPGKHGFHIHEFGDCSAPDGTSAGGHFNPDSHEHGA
                     PGVTAHPGDLGNLEADANGRATLSLVLDQVTLDAGPLSIAGRGLIVHADPDDLMSQPV
                     GLAGARVACAVIMDDAGDTKPVLQAM"
     gene            4819..>5123
                     /locus_tag="VGB85_05095"
     CDS             4819..>5123
                     /locus_tag="VGB85_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133429.1"
                     /translation="MSFITLRSSCLLATSVLLACGAEPDDEGADGDDSGSTAPTGAPG
                     DTSTSTPTSSSTTGDDSSDDVPTTTGGDGSDSQGSASGTTATTGDDDTTGASEWDGEP
                     "
BASE COUNT          821 a         1893 c         1690 g          719 t
ORIGIN      
        1 cgcggcggcg gcccgctgag cctgcgcgcc cgcagcggcc ccggcgagga gccggacaag
       61 cgctggagcg agtggaagac cgtcacgctg gccaagcagg gtgatgcatt gcgcggcgca
      121 ctggcggtgc cgggtcggcg ctacgtgcag ctcgaggtcg gcctcgacgc ggtcagcgag
      181 ctgcgcgaca tctcgctctt ctatgcgccg gagaacctcg cgccgctggt ccagagcgtc
      241 gacatcgccg gtccggagat cgacgacgag gacgagggcg agcccgaccc caagctgacg
      301 atcaagtgga aggccgaggc gcgcgacgac gacgagctcg tctacgaggt caggatccgt
      361 cccgagggca gcgacgccac ccagtggatc aagctcggcg gcgagaaccc ggtcaccaag
      421 aaggagctca agtgggacct cggcagcgtg cccgacggcg tctacgaggc gcagatcgtc
      481 gccagcgacg agccgagcaa cggctccgcc cgcgcccgca ccgacgaggt cgtcagcgcc
      541 cccttcatcg tcgaccacaa gcgcccgacg atcgacgagg tcaaggtgca gggcgcccgg
      601 atcgtcgccc gcgcgcgtga cgagggcagc cggatcctcg gcgtctccta cagcatcgac
      661 ggcggggcct tccgcgccgc cagccccgcg gacggcctgt tcgacggcga cagcgagacc
      721 ttcgaggtca ccctccccgc cgacctcaag cccggcaaac accgcgtggt cctccgcgcc
      781 cgcgatgcct tcggcaacct cggcaccgtc gccgtggtcg ccacgaccga ccgctgaggc
      841 gccgctcgcc ggccttcgtc ggcggcagaa caatgctccc gctgcacaga cgggccgcgt
      901 cgccatgcgt gatgcatttg gtccccgtcg tcgatacgct gcggcgacct cgctcgcggg
      961 tcgcgagcag gaaggccagc atctatgaac aatcgtatcg ctcccggtat catcctcggc
     1021 atattcacat cctccgtcgc ccacgcagag ccgtgggtcg aggacaaggc ttttttcacc
     1081 gagacccacc aatccggctc gagcatggtc gacgccgccg acctcgacgg ggacggatgg
     1141 atcgacctcg tgttcgccaa cggcggcggc tacaacaagg gcagcctcga caatgattta
     1201 aagcagcagg cttttcataa cgaggccggc gcggcgatga ccccgatcga aagcccggac
     1261 atcttcggcg gcgtcacgta taccggccgc gccgtcaaga tccgggacat cgacaacgac
     1321 ggcgacaacg acatcttcct cggcgtcacc tggcaggggc agagccagct gttcgtcaac
     1381 gacggcgccg gcaacttcgt caacgagacc gcgatcaatc tccctgcgat caacgccagc
     1441 gtcggtgacc tcgaattcgg cgacatcgac gacgacggcg acctcgacgt gatgctcgcc
     1501 gactggggcc ccgactcgcc cgtcgacgac cctatcaacc cgggcggcgt cacccggctg
     1561 tggctgcaag acggcgcgcc cggccacttc ggcccgggca ccgcgatgtt cagcgacttc
     1621 acggtcgacc agatgccgga catcggcatc cgctggtcgt gggacctcga gttcgtcgac
     1681 ctcgacaacg actacgacct cgacgccgtc gtgtcctgct tcgcgtgcac cgggtccagc
     1741 gtgcacctgt tcgccaacga cagcgccggg accttcaccg ccgtgccgct cgccaacgcc
     1801 ccgcagggcg ccggtgcgct cagcgtcgag gcgatcgacc tcgacggcga cgccttcctc
     1861 gacctcgtca ccacgcacga cgccgcgggc ggtcgcaacc gcgtgctgct ctccgacggc
     1921 ctgggggcct tcagcgacgc gagcctcgtg cactggccgg cgtccgagaa cccggccagc
     1981 tacgaccaca tggcggcgtt cctcgaccac gactccgacg gccggccgga catcgtcctc
     2041 ggcgccttgc agccgggcct caacaagtac cccgaccgcc tgatggttaa tcaaaacggg
     2101 aagttcacgg ccaacaacgc cgcgttcgcc gagatcaagg cgtccagcgg cacttacgcg
     2161 atcgtgctcg ccgacttcaa ccgcgaccac cgcctcgacg tcgccatggc ccagaacgag
     2221 aacgccttcg acaagaaggt gttcctcgcc agcgatgtcg agcggacgcc cgatacgctc
     2281 gcgccgcgct tcatcaacca cgaggcgctg cccgccgacc tcgagttcgg ccaggaccac
     2341 acgctcaggc tgcgctgtca cgacaacaag tcgccgctga tgctgcacga cttccagcgc
     2401 ggcgtcgacg acatgcccga cggccgcccc ttcgtcgagt catggaacac gaccccggcc
     2461 gaccccgata ccaccccgcc ggacgagacc tccgcccccg gccagtggta cggcgaatac
     2521 ctgtggcgcg tccgcttcag cgtaccgaag caggccgcct tgttcgccta ccgcatctgt
     2581 gccatcgacg ccgccggcaa caagggctgc acggagctcg ccagcgttac caaccaagac
     2641 gccggcacca ccggcgacct cgacacgacc accggcaccg ccggcagctc cagctcgacg
     2701 agcgacaccg gctcgacgag cagcgccggg accaccacga ccgacccggc gcccactggc
     2761 accaccccca ccggcacgac cgggccctcc gataccccga ccaccggcgg tcaggacgac
     2821 ggcagcgccc ccatgacgac caccgcggcc agcagctcat ccagcgacac cgccccccac
     2881 gaccccggga ccgacggcgg ctgtagttgc aacggccgac cggggccccg gagcgggctg
     2941 ttcgcgctgc tgctggtcct gtgcacgcgt cggcgccggg cctgatcgcg gtggaccggc
     3001 gcgcttgcgg cctggcatgt cccgctgcat cctctctcgc catgtcgatc gccgccgagc
     3061 tcgccgcgtc ccctccgagc gccctcgcgg cccggatggc gcggctgcgc tggtcggact
     3121 tcgggctcga ccttgcgtcg cagctcccgc gtcggctctg gtccagtcac aagctgcact
     3181 tcgatcgcgc ctgcgagatc gggctgttct tcctcccccg cggcgagcgg atcccgctgc
     3241 acgatcaccc gcacattcac gtgtggatgc gggtgctgtg cgggcggctg cgggcgacca
     3301 gcttcacctg ggccgctcga ccgctcgcgc ggcgcagcgg cgacgcggtg ctcgacgcgg
     3361 gctcgccggt gtgggcggtg ttgccggagc aggacaactt gcacgagctc gaggcgctcg
     3421 aggacgtcgc cttcctcgac gtgctgcgcc cgccgtacat cgacggccgc gtctgcacgt
     3481 actacgcggc cgcgcccgcg cacgacgacc tgtggcacat gacggcgctc ccctgatcag
     3541 gtccgatcag gtcccggcga gcgccgcgat gcaggcctcg cgcacgcgcc acaccgagtc
     3601 gagggtccag cctggcaggt tcgtcgtcat ccactgggcc aggaagcggc ggtggatggc
     3661 gaccttgtgg gtgacgatgt cctcgggccg gatgcgctcc tcgtggatga agctcggctc
     3721 gcgcgccccg gcgcgggtca ggtcgatgta ctggccgttc cagcgcagga cgcagtgggc
     3781 ctcggggatc gccgtgaggc cgtgggccgc gagcacgctg ccgacgcccg gcgtgttgtg
     3841 accgtccatg agatagatgc cgagcaccag ctcgacgtcg acgccgtgct cgcgggccag
     3901 gcgggccagc agagcgtgct tggtgctgca ggtgccgcgg ccctcgtgga ggatcgccag
     3961 gtcgtcgcgg tcggccccgc gcccgtaagg cagctgcagg gcccacagcg ccacctcgtg
     4021 gtagctggtc atcccgcgcc cgcggcagcc cacggtcgcc ggacccgccg agacgagggg
     4081 gaagtcgggc agccgtgtcg gcatgcccga gtttatcgcg aaaccgaacg cgcgcgccgt
     4141 cgttacatcg cctggagcac cggcttggtg tcgccggcgt cgtccatgat cacggcgcag
     4201 gcgaccctcg ccccggccaa accgaccggc tggctcatca ggtcgtcggg gtcggcgtgg
     4261 acgatcaggc cgcggccagc gatgctcagc gggcccgcgt cgagcgtcac ctggtcgagc
     4321 accagcgaga gcgtcgcccg gccgttggcg tcggcctcga ggttcccgag gtcgcccggg
     4381 tgggccgtca cgccgggggc cccgtgctcg tggctgtccg ggttgaagtg gccgcccgcc
     4441 gacgtcccgt cgggcgccga gcagtcgccg aactcgtgga tgtgaaagcc gtgcttgccg
     4501 ggcgtcagcc ccgagatgtc ggcctcgacg agcaccttgc cgtcgctggt ggcagtgaag
     4561 gtggccttgc cggtcacgtt gctgtccccg agcgcctgga gctccgcggt ggcagactcg
     4621 ctgtcggcgt caccgccgca gcccgcctgg aacgccgtca tcgccacgat caggggtagg
     4681 acgtaatttc gcaaagaggt catgactcga tcatcatgcg accggtcgtg ggtcgttgcc
     4741 aatggctgtg tgcaagatcg cgccgatctc acgcaatgca gggttttatc gccgcgatcc
     4801 cggctacgct gccggatcat gagcttcatt acgctccgat cctcctgcct cctcgcgacc
     4861 agtgtcttgc tcgcatgcgg cgccgaacct gacgacgagg gtgctgatgg cgacgacagt
     4921 gggtccacag cccccaccgg ggcgcctggt gacacatcga cgagcacccc gacgagcagc
     4981 tcgacgaccg gcgacgacag cagcgacgac gtgccgacga ccaccggcgg cgacggcagt
     5041 gacagtcagg gatctgccag tggcacgacc gcgaccaccg gcgacgacga cacgaccggc
     5101 gcgagcgagt gggacggcga gcc
//
LOCUS       DASWNJ010000282         5017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_253175, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000282 DASWNJ010000000
VERSION     DASWNJ010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253175"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..504
                     /locus_tag="VGB85_05100"
     CDS             190..504
                     /locus_tag="VGB85_05100"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0133430.1"
                     /translation="MGTLLSLALYTPSEVARQLGERMAQLRLARNWKRETLAERAGVS
                     VFLIKRFESGGQVSLENLLKLALALGCLDQFEALLKPPPARSLAELDRQTSAPPRRRG
                     TQ"
     gene            501..1793
                     /locus_tag="VGB85_05105"
     CDS             501..1793
                     /locus_tag="VGB85_05105"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HEY0133431.1"
                     /translation="MNVTTRLEVRLERTPGDVEVVGQLALHRGQVLLELAADFITGPR
                     SISPFKLAMQPGLQPAPHGLRHGLHGVFDDSLPDGWGLLLMDRVFLARGVRPGELSAL
                     DRLAYLGRRTLGALTYHPCSEPDTGAPEAVRLGELASAALAVHEGGATEVLPALQRAG
                     GSPGGARPKVLVGLLGDRFISGADDLPPGYRPWLIKFNTRDDGADAGALEYVYARMAR
                     AAGITAPEARLFDLPNGERCFGVRRFDRDDDGRVHMHTLANLLHVDYRTPSLDYEHLI
                     RVTTQLTRNHSHVRECFRRALFNWLAHNRDDHGKNFAFLMRRDGTWDLSPAYDLTFAE
                     GAGGEHTTTYAGQGKRPRWTDLQRLARLASIDAADALQILAQVREGVATWASEAAALQ
                     VPSAQVREIAGRLAEVEAAGELPPRTPAPRSPGRRARP"
     gene            1790..3133
                     /locus_tag="VGB85_05110"
     CDS             1790..3133
                     /locus_tag="VGB85_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133432.1"
                     /translation="MTRLRALLVFLAAFVVLAVLAGPELLRPGRNNHFVHMARGWLEG
                     RLALADVPPGYPRAHDDWGRVWTLGLRDGTSLRGDPCRTAECDALRRTGIETWRPTTG
                     PAREVPRRDIVTRASTWYVTFPPGPALLMLPGVALWGLGFLDVLFTALAGALIPVVLV
                     RLLGRVRGPGHLREHLWAAAAWTVASPACFVAAHGSVWFTAQVLGSLALFLYIEAAWD
                     MRRPATAGLWLGLAVACRVSIAFAVIFAALEWSRDRRLATLLRFVAPLALIGTALAWF
                     NWARFDDPLEFGHRYLDIRWQTRIQTLGMFSPSYLMRNLECMLWLAPQVSPQLRWSIH
                     GMGLLLASPWLVLLARARARFPQRLGLALAAVAVAVPALLYQNSGQVQFSYRFALDFL
                     PLLLVLLVVGGGARGRSFPALVILAAVVQLYGAWMYGRAPGRLFVQDPWWPFAPE"
     gene            complement(3140..3913)
                     /locus_tag="VGB85_05115"
     CDS             complement(3140..3913)
                     /locus_tag="VGB85_05115"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0133433.1"
                     /translation="MTEPLRLITIGFSHYCEKARWALDRGDLPYREEDHVPLFHWRGT
                     LRAGGGRTVPVLVTPDGVLRESTAIVEYVDENLARDRRIIPEDAKERAEVMTWIADFD
                     RGLGPAVRRYLYFHLLHDREATAELLSSTGPSWERTLARRLFPVIRAAIVRGLKISPA
                     SAERSRQRIESSLAAVDARLADGRRFLVGDRFSAADLTLAALACPLIQPRQAGFELPQ
                     RCTQLPGLAAAVAAARATIAGQHVLRLYAQERPPVRALA"
     gene            complement(3928..4218)
                     /locus_tag="VGB85_05120"
     CDS             complement(3928..4218)
                     /locus_tag="VGB85_05120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HEY0133434.1"
                     /translation="MPKISISRNHSLPPAVLKQRLVDLGEKLQAKYQAKTSWEGDKTL
                     NVKGPGVEGKLLIGDNKVDVNLDLGFLLSPMKGKIEEALTKELESVTKPETA"
     gene            4337..>5017
                     /locus_tag="VGB85_05125"
     CDS             4337..>5017
                     /locus_tag="VGB85_05125"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEY0133435.1"
                     /translation="MLQSVTATATTPASNPAAPATASAATSPATSPGDTATATARAKP
                     AGRATATTARPTAAAVPPPDPFWRSGDGRVRLFKGDCVSVLDALPAESVDVIFADPPY
                     FLSNGGTTCRAGKRTSVDKGAWDRSQGVEENHAFNHKWLAACNRVLKPNGTIWVSGTG
                     HVIYSVGYAMQQQGFKPLNEIVWEKPNPPPNLSCRYFTHSTETILWAARNRKSRHHFN
                     YDTMRELNG"
BASE COUNT          670 a         1855 c         1697 g          795 t
ORIGIN      
        1 tcttcgcgcc cggcagcaac cacacgatca ccgcgagccc gctctactac tcgaccaccg
       61 tcgacggcgt gcgcctcgtc gactggatct ccgacctgct cgccgggcag accgcgcacg
      121 tcagtccgtg atatttttca cgtaaataac tgataatctg ttgtctcttc gcccatcttt
      181 tggcctgtaa tgggcaccct gttgtccctt gctctctaca cgccgtccga ggtcgcgcgg
      241 cagctgggcg agcggatggc gcagcttcgc ctggctcgca actggaagcg cgagaccctg
      301 gccgagcgtg ccggcgtgtc ggtattttta ataaagcgct tcgagtccgg cggtcaggtc
      361 tcgctcgaga acctgctcaa gctggcgctc gcgctcggct gtctcgatca gttcgaggct
      421 ttattaaaac caccgccggc gcgctcgctc gccgagctcg atcgccagac cagcgcaccg
      481 ccgcgtcgtc gaggcaccca gtgaacgtca ccacgcggct cgaggttcgc ctcgagcgca
      541 cgcccgggga cgtcgaggtc gtcggacagc tcgcgctgca ccgcggacag gtcctcctcg
      601 agctggccgc ggatttcatc accggaccga ggtcgatctc tccgttcaag ctggcgatgc
      661 agccgggcct ccagccggcc ccgcacgggc tgcgtcacgg gctgcacggc gtgttcgacg
      721 actcgctgcc ggacggctgg ggcctgctgt tgatggaccg cgtgttcctg gcccgagggg
      781 tccgacctgg cgagctctcc gcgctcgacc gcctcgcata tctcggccgg cgcacgctcg
      841 gcgcgctgac ctaccatccg tgcagcgagc ccgacaccgg cgcgcccgag gccgtacggc
      901 tcggcgagct cgccagcgcc gcgctcgcgg tgcacgaggg cggcgcgacg gaggtgttgc
      961 cggcgttgca acgggccggc ggttcacccg gtggcgcgcg gcccaaggtg ctcgttgggc
     1021 tgctcggcga tcgcttcatc tccggcgccg acgacctccc gcccggctat cgcccgtggc
     1081 tgatcaagtt caacacccgc gacgacgggg cggatgccgg cgcgctcgag tacgtctatg
     1141 cgcgcatggc ccgggcggcc ggcatcaccg cgccggaggc ccgcctgttc gacctgccga
     1201 acggcgagcg ctgcttcggc gtgcgccgct tcgaccgcga cgacgacggc cgcgtgcaca
     1261 tgcacaccct cgccaacctc ctgcacgtcg actaccgtac gccgagcctc gactacgagc
     1321 acctgatccg cgtcaccacg cagctgacgc gcaaccacag ccacgtgcgc gagtgcttcc
     1381 ggcgcgcgct gttcaactgg ctcgcccaca accgcgacga ccacggaaag aactttgcgt
     1441 tcctgatgcg ccgcgacggt acctgggatc tctcaccggc ctacgacctg accttcgccg
     1501 agggcgccgg cggcgagcac accacgacct acgcggggca aggcaagcgg ccccggtgga
     1561 ccgacctgca gcggctcgcg cggctcgcct cgatcgacgc tgcggatgcg ctgcaaattt
     1621 tggcgcaggt gcgcgagggc gtcgcaacct gggccagcga ggccgccgcg ttgcaggtcc
     1681 catccgcgca ggtccgcgag atcgccggcc gtctcgccga ggtcgaggcc gccggggagc
     1741 tcccgccgcg gacgcccgcg ccgcggtccc ccggacggag ggccaggcca tgacgcgcct
     1801 gcgtgcgctg ctggtgttcc tcgccgcgtt cgtggtgctc gccgtgctcg ccgggccgga
     1861 gctgctgcgg cccggtcgca acaaccactt cgtgcacatg gcccggggct ggctcgaggg
     1921 gcggctcgcg ctcgccgacg tgccaccggg ctacccgcgc gcgcacgacg actggggccg
     1981 cgtgtggacc ctcggcctgc gcgacggtac cagcctgcgc ggtgatccct gccgcaccgc
     2041 cgagtgcgat gcgctgcggc gcaccggcat cgagacctgg cgaccgacca ctggccccgc
     2101 gcgggaggtc ccccggcgtg acatcgtcac gcgcgcgagc acctggtacg tcaccttccc
     2161 gccgggcccg gcgctgctga tgctgcccgg cgtcgcgctg tggggcctcg gcttcctcga
     2221 cgtgctgttc actgccctcg cgggcgcgct gatccccgtc gtgctcgtgc gcttgctcgg
     2281 gcgcgtgcgc gggccaggac acctgcgcga gcacctgtgg gccgccgccg cgtggacggt
     2341 ggcgagcccg gcctgcttcg tcgccgcgca cggcagcgtg tggttcaccg cgcaggtgct
     2401 cggcagcctc gcgctgttcc tctacatcga ggccgcctgg gacatgcgcc ggcccgccac
     2461 cgccggtctg tggctcggcc tcgcggtcgc gtgtcgcgtc tcgatcgcct tcgcggtgat
     2521 cttcgcggcg ctcgagtggt cccgcgaccg tcgcctcgca accctgctgc gcttcgtcgc
     2581 cccgctcgcc ctcatcggca cggcgctcgc gtggttcaac tgggcccgct tcgacgaccc
     2641 gctcgagttc ggccaccgct acctcgacat ccgctggcag acgcggatcc agacccttgg
     2701 catgttctcg ccgagctacc tcatgcgaaa cctcgagtgc atgctgtggc tcgccccgca
     2761 ggtctcgccg cagctgcgat ggtccattca cggcatgggc ttgctgctcg cgtcgccgtg
     2821 gctggtgctc ctggcgcgcg cgcgcgcacg gttcccccag cgcctgggcc tggcgctcgc
     2881 cgccgtcgcc gtcgccgtgc cggcgctgct gtatcagaac agcgggcagg tgcagttcag
     2941 ctaccgcttc gcgctcgact tcctgccgct cttgctcgtc ctgctcgtcg tcggtggtgg
     3001 cgcgcgcggg cgatcgttcc ccgcgctcgt catcctcgcc gcggtcgtgc agctgtatgg
     3061 cgcgtggatg tatggccgcg cgcccggtcg cctgttcgtg caggacccgt ggtggccgtt
     3121 cgctccggaa tgaaccgact caggcgagcg cgcgcaccgg aggccgctcc tgcgcgtaga
     3181 ggcgcagcac gtgctgcccc gcgatcgtcg cccgggccgc cgcgaccgcg gccgcgaggc
     3241 caggcaattg ggtgcatcgc tgcggcagct cgaagccggc ttgcctcggt tggatcagcg
     3301 ggcacgcgag ggcggcgagg gtcaggtcgg cggcgctgaa gcggtcgccg acgaggaagc
     3361 gacgtccgtc cgcgaggcgg gcgtcgaccg ccgcgaggct gctctcgatg cgctggcgcg
     3421 agcgttcggc cgaggccggc gagatcttca ggccacggac gatcgccgcg cggatcaccg
     3481 ggaacaggcg gcgggccagc gtgcgctccc acgacgggcc ggtcgagctc agcagctcgg
     3541 cggtcgcctc gcggtcgtgc agcaggtgaa aatacagata ccggcgcacc gcggggccga
     3601 ggccgcgatc gaagtcggcg atccaggtca tcacctcggc ccgctccttc gcgtcctccg
     3661 ggatgatcct gcggtcgcgg gcgaggttct cgtcgacgta ctcgacgatc gcggtcgact
     3721 cgcgcaggac cccgtccggg gtcacgagca cgggcaccgt gcgaccgccg ccggcccgga
     3781 gggtgccccg ccagtgaaac agcgggacgt ggtcctcctc gcggtacggc aggtcgccac
     3841 ggtcgagggc ccagcgggcc ttctcgcagt agtgactgaa gccgatcgtg atcaggcgca
     3901 gcggctcagt catgatcggc cttcacttca ggccgtctcg ggcttggtga cgctctccag
     3961 ctccttggtc agcgcctcct cgatcttgcc cttcatcggc gagagcagga agccgaggtc
     4021 gaggttgacg tcgaccttgt tgtcgccgat caggagcttg ccctcgacgc cggggccctt
     4081 gacgttgagc gtcttgtcgc cttcccacga ggtcttggcc tggtacttcg cctgcagctt
     4141 ctcgccgagg tcgacgaggc gctgcttgag cacggcgggg ggcagggagt ggttccgcga
     4201 gatcgagatc tttggcataa gacgcctcct ccataacaaa tgctcgccga cacggcaaac
     4261 gacccgctcg attaacggtg cgcgggcgtt cggcggaaaa tttgggcggc gagcgcgggc
     4321 cggcgatggt gcgcgcgtgc tccaaagcgt gaccgccacc gccacgacgc cggcctccaa
     4381 cccggccgct cctgccacgg ccagcgcggc tacctccccg gcgaccagcc cgggcgacac
     4441 cgccaccgcc accgcccggg cgaagcccgc aggacgcgcc acggccacca ccgcgcgtcc
     4501 gaccgcggcc gccgtgccgc cgccggaccc gttctggcgc tcgggcgacg gtcgggtccg
     4561 cctgttcaag ggtgactgcg tcagcgtgct cgacgcgctg ccggccgagt cggtcgacgt
     4621 catcttcgca gatcctcctt acttcctcag caacggcggg accacctgcc gcgccggcaa
     4681 gcgcaccagc gtcgacaagg gcgcgtggga ccgcagccag ggcgtcgagg agaaccacgc
     4741 gtttaaccac aagtggctcg ccgcctgcaa ccgcgtgctc aagcccaacg ggacgatctg
     4801 ggtctcgggc accggccacg tgatctactc cgtcggctac gcgatgcagc agcagggctt
     4861 caagccgctc aacgagatcg tgtgggagaa gccgaacccg ccgccgaacc tgtcctgccg
     4921 ctacttcacc cactcgaccg agacgatcct gtgggccgcg cgcaaccgca agagccggca
     4981 tcacttcaat tatgacacca tgcgcgagct caacggc
//
LOCUS       DASWNJ010000283         4302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_288127, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000283 DASWNJ010000000
VERSION     DASWNJ010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288127"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VGB85_05130"
     CDS             complement(<1..442)
                     /locus_tag="VGB85_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY0133436.1"
                     /translation="MSHVDYQADAGIALITLNVPPANCYSYELMQALDAAILRARFDA
                     GVHVILLTGAGEKFFCAGADIAMLQGADPEFKYNFCLHANETISRLEQTPKLVIAALN
                     GHCVGGGLEIALAADLRVARRGPGKCGLPEVKLGVLPGTGGTQRL"
     gene            486..947
                     /locus_tag="VGB85_05135"
     CDS             486..947
                     /locus_tag="VGB85_05135"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY0133437.1"
                     /translation="MHVHRVEVRFGDCDPAGIVYFPRFFDLFHQAMETWFAAGLGLAY
                     ASLILERRLGFPAVHSEADFKVPCALGEAIEIELRVGRLGRSSIGLEYVARGVLGDVR
                     LTGATTSVVMDLDPASPGFRRAVPIPDDLRARIQRFMTGGGDGDEPVPGDI"
     gene            958..2346
                     /locus_tag="VGB85_05140"
     CDS             958..2346
                     /locus_tag="VGB85_05140"
                     /inference="COORDINATES: protein motif:HMM:NF014697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HEY0133438.1"
                     /translation="MAAALTWLPALGVAARRGFHRVTPEPFAIAVLLTVLVLIASAVQ
                     LGGTPEALAQTLTLWQRPGGLWSLLGFGMQMSVMLVLGSALADAPSIRGALHRLARAC
                     GGPRRLVGVTALLSCTLALINWPFGLVCGAVFARSGGQEAARRGWRLHYPLLCAAGYS
                     GMMVWHGGLSGTAPLKATTLADLREVLGPALADQVGPIALTESLFGGLNLWVTGGLWI
                     LAPLLFMAMTPADGADPRPAAAPVDPEPVLADSEPASDWLARLERSRVVTWLLAAPLA
                     AAFALYVQAQGLATLDLNTVNLGLWLLALVLHGRPDRFLRACDAGVRACTGIILLFPL
                     YGGIMGMLSGSGLSGLIAARFAEAGAGVFTWITFLSAGLLNLFIPSGGGQWAVQGPVV
                     MAAALARDVPPAHAMLAIAYGDQWTNMLQPFWAAPLLAITGVRARDITGYCALWLLAG
                     GLWISACLLLHA"
     gene            complement(2347..2673)
                     /locus_tag="VGB85_05145"
     CDS             complement(2347..2673)
                     /locus_tag="VGB85_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133439.1"
                     /translation="MHRLFSTLILALLACTAVACDDGGPDSGDAESETTTTDTDADTD
                     PTGDATGEPGTAGAATVGTLGQPSGGGPTTGDTTGDTTGDDGDTGHATQGPTTLAPPT
                     IEQQDE"
     gene            3085..>4302
                     /locus_tag="VGB85_05150"
     CDS             3085..>4302
                     /locus_tag="VGB85_05150"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0133440.1"
                     /translation="MTDTHDASPADRSPKSRVGTTFAGEFNLLKFLGRGDVADTFEAE
                     PLDQPVLVIVKIVRAELAADRAQVELLLREARDTSAIVHDNVAQVLLADISPEGEPYI
                     VAEHLEGEDLGIILRRIGRLPWPRVRRLGLQIVAGLRAAHEAGVLHRVIKPSNVMVLS
                     DDTVKLVDFGLVRLESVTADREPGTVVLGDAMHFIAPEQALGEQVDARTDIYSVGVLL
                     YTLLTGRVPFFGRPTQVAMQHKFSTAAAPGSVAPEAGIPAVVEALILKAMSKRRDDRF
                     PDMAAFEAALQALGDDGKRGAAAGEASRVQPTTDSAMFYSLEALQAMLASTQDPRAQL
                     GIYEKMTKALVTLAGGKDSAEADAWRAWIAQGRDYAYYQLAEAHLAAERWTDLVQVYR
                     EHADSTADRTMKIG"
BASE COUNT          536 a         1562 c         1532 g          672 t
ORIGIN      
        1 ccagtcgctg cgtgccgccg gtacccggca agaccccgag cttgacctcc gggagcccgc
       61 acttgccggg gccgcgccgg gcgacccgga ggtcggcggc cagcgcgatc tcgaggccac
      121 cgccgacgca gtgcccgttg agcgccgcga tcaccagctt gggcgtctgc tcgaggcggc
      181 tgatcgtctc gttggcgtgc aggcagaagt tgtatttgaa ctcggggtcg gcgccctgca
      241 gcatcgcgat gtcggcgccg gcgcagaaga acttctcgcc cgcgccggtc agcaggatca
      301 cgtgcacgcc ggcgtcgaag cgggcgcgca ggatcgccgc gtcgagcgcc tgcatcagct
      361 cgtaggagta acagttggcc ggcgggacat tcagcgtgat cagcgcgatg ccggcatcgg
      421 cctggtagtc gacgtgggac atgggcgcag catacactcg ccgcggtccc atgttgatgc
      481 gtggcgtgca tgtccaccga gtcgaggtcc gcttcggcga ctgtgatccc gccgggatcg
      541 tctacttccc gcggttcttc gacctgtttc atcaggcgat ggagacctgg ttcgccgccg
      601 gactcgggct cgcgtacgcc agcctgatcc tcgagcgcag gctcggattc ccggcggtcc
      661 acagcgaggc cgacttcaag gtcccctgcg ccctcggcga ggccatcgag atcgagctca
      721 gggtcggccg cctcggccgc agctcgatcg gcctcgagta cgtcgcgcgc ggcgtcctgg
      781 gcgacgtgcg cctcaccggt gcgaccacct cggtcgtcat ggacctcgac cccgcctcac
      841 ccgggtttcg ccgcgccgtg ccgatccccg acgacctgcg cgcccggatc cagcgcttca
      901 tgaccggcgg tggcgacggt gacgaacctg tccccgggga catctaggag aacagcgatg
      961 gccgcggcgc tgacatggct accggcgctc ggcgtcgcgg cgcggcgcgg gtttcatcgc
     1021 gtcacgcccg agcccttcgc gatcgccgtg ctgctgaccg tcctcgtgct gatcgccagc
     1081 gccgtgcagc tcggcgggac acccgaggcg ctcgcccaga ccctgacgct gtggcagcgc
     1141 ccgggcggcc tgtggagcct gctcggcttc ggcatgcaga tgagcgtgat gctcgtgctc
     1201 ggctcggccc tcgccgacgc cccatccatt cgcggcgcct tgcacaggct cgcgcgggcc
     1261 tgcggcgggc cccgacgtct cgtcggcgtc accgcgctgc tgtcgtgcac gctcgccctg
     1321 atcaactggc cgttcgggct cgtgtgcggc gccgtgttcg cgcgcagcgg cgggcaggag
     1381 gccgcgcgtc gcgggtggcg gctgcattac ccgctgctgt gcgccgccgg ctacagcggc
     1441 atgatggtgt ggcacggtgg tctgagcggc acggccccgc tcaaggcgac gaccctcgcc
     1501 gacctgcgtg aggtgctcgg tccggcgctc gccgaccagg tcggcccgat cgccttgacg
     1561 gagagcctgt tcggcgggct caacctctgg gtcactggcg ggctgtggat cctcgcgccg
     1621 ctgctgttca tggcgatgac cccggccgac ggcgccgacc cgcgacccgc ggccgcgccg
     1681 gtggatcccg aacctgtcct cgcggacagc gagccggcga gcgactggct cgcgcggctc
     1741 gagcgctcgc gcgtcgtcac gtggctgctc gccgcgccgc tcgccgccgc gttcgccttg
     1801 tacgtgcaag cccaaggcct cgcgaccctc gacctcaaca ccgtcaacct cggcctgtgg
     1861 ttgctcgcgc tggtgttgca cggccggccc gaccggttcc tgcgcgcctg cgacgcgggc
     1921 gtgcgcgcgt gcaccggcat catcctgctg ttcccgctgt acggcgggat catgggcatg
     1981 ctctccggtt cgggtctgtc gggcctgatc gcggcgcgct tcgccgaggc cggcgctggc
     2041 gtgttcacgt ggatcacgtt cctgtcggcg ggcctgctca acctgttcat cccctcgggg
     2101 ggcgggcagt gggccgtgca gggtcccgtc gtcatggccg ccgcgctcgc gcgcgacgtg
     2161 ccgccagcgc acgcgatgct ggcgatcgcc tacggcgatc agtggaccaa catgctgcag
     2221 ccgttctggg ccgcgccctt gctggcgatc accggcgtgc gcgcgcgcga catcaccggc
     2281 tactgcgcgc tgtggctgct ggcgggcggc ctgtggatct ccgcctgcct gctgttgcac
     2341 gcctgatcac tcgtcttgct gctcgatcgt cgggggcgcg agggtcgtcg ggccctgcgt
     2401 cgcgtggccg gtgtcaccgt cgtcgccggt cgtgtcgccg gtcgtgtcgc cggtcgtggg
     2461 gccgcccccg gacggctgac cgagcgtgcc gacggtcgcc gcaccggcgg tcccgggctc
     2521 acccgtcgcg tcgccggtcg ggtcggtgtc ggcatcggtg tcggtcgtgg tcgtctcgct
     2581 ctcggcgtct ccactgtcgg ggccgccgtc gtcacaggcg acggcggtgc aagcgagcag
     2641 ggcgaggatc agggtagaaa acaggcggtg catggtggtc tccggggggc gggtcgcaag
     2701 ggatgtcgcc gggccgcacc tcgatgtgtg cgacagggac acgcgccgtc gggcgcgggg
     2761 tgaggcggcc gtgacggccg cagatctcga gctggcctct cggacaggcg cgctgcgctg
     2821 gcagctcgcg gcgagtggca cgtctctcct gtaagaccga ggtctcggcg ccacagtttc
     2881 acggccgtca cgatcgtgtt caccaccgct gcgtcccacg cccctccggg aaatcgcccc
     2941 gtcgccggcg cggccctgaa cagtggctcc gcggcggcgc ccgcgaggcg tgtgtcgatg
     3001 tacaggcaag ggcgcttgcg cgcggtcgtg gccgatcggg cgccacgtgg gtggcgaggc
     3061 gatcgggtat ggttgacgct gcccatgacc gacacgcacg acgcttcgcc cgcggatcgc
     3121 agcccaaaga gccgggtcgg cacgaccttc gccggcgagt tcaacctgct caagttcctc
     3181 ggtcgtggcg acgtcgccga caccttcgag gccgagccgc tcgatcagcc cgtgctcgtg
     3241 atcgtcaaga tcgtgcgcgc cgagctggcg gccgaccggg cccaggtcga gctgctgctg
     3301 cgtgaggccc gcgacacctc cgcgatcgtg cacgacaacg tcgcccaggt gctgctcgcc
     3361 gacatcagcc ccgagggcga gccctacatc gtcgccgagc acctcgaggg cgaggacctc
     3421 ggcatcatcc tgcgccggat cggccggctg ccgtggccgc gggtgcgaag gctcggcctg
     3481 cagatcgtcg ccgggctgcg ggcggcccac gaggccggcg tgctgcaccg cgtcatcaag
     3541 ccgagcaacg tgatggtgct gtccgacgac accgtcaagc tcgtcgactt cgggctcgtg
     3601 cgcctcgaga gcgtcaccgc cgaccgcgag cccggcaccg tcgtgctcgg cgacgccatg
     3661 cacttcatcg cccccgagca ggcgctcggc gagcaggtcg acgcgcgcac cgacatctac
     3721 tccgtcggcg tcctgctcta tacgctgctg accggccgcg tgcccttctt cggaaggccg
     3781 acgcaggtgg ccatgcagca caagttctcg accgccgccg cgcccggcag cgtcgccccc
     3841 gaggccggca tcccggcggt cgtcgaggcg ctgatcctca aggcgatgtc caagcgccgc
     3901 gacgatcgct tcccggacat ggccgccttc gaggcggcgc tgcaagctct gggcgacgac
     3961 ggcaagcgcg gcgccgcggc gggcgaggcc tcgcgcgtgc agccgaccac cgacagcgcg
     4021 atgttctact cgctcgaggc cctgcaggcg atgctggcga gcacccagga cccgcgcgcg
     4081 cagctcggca tctacgagaa gatgaccaag gcgctggtca ccctcgcggg cggcaaggac
     4141 agcgccgagg ccgacgcctg gcgggcctgg atcgcccagg gccgcgacta cgcctactat
     4201 cagctcgccg aggcgcacct cgccgccgag cgctggaccg acctcgtgca ggtgtaccgc
     4261 gagcacgccg acagcaccgc cgaccgcacg atgaagatcg gc
//
LOCUS       DASWNJ010000284         3149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_111394, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000284 DASWNJ010000000
VERSION     DASWNJ010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111394"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VGB85_05155"
     CDS             <1..550
                     /locus_tag="VGB85_05155"
                     /inference="COORDINATES: protein motif:HMM:NF024100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0133441.1"
                     /translation="ARLAALHNNMAGLAVDDGRLADAVPDYRSALEGFRAAGPDNEYT
                     ITVEANLAETLLALGQADEALALQQHVVAVRERESAGDPAAAVARVQQAQIVRALGRP
                     EEAVALLEAALAILESAPVEHRWRRGLGRYTLARCLAELRRDRPRQQLLASLAADDLA
                     SDGDYFARRALLELRTWLTPSR"
     gene            707..1336
                     /locus_tag="VGB85_05160"
     CDS             707..1336
                     /locus_tag="VGB85_05160"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HEY0133442.1"
                     /translation="MPSISILGTGRMATTLARCWLEKGNSVTLGSRNPEQARTRIGAL
                     AEQLTVTDFASACASAEVVALAVPYHAAGDVIAAAGDLEGKIIIDTTNPWSPDSHHLA
                     VAGDTSGAEEIARRARGAHVVKALNGIFWKNLKDPTFSGLPASMFYCGDDDSSKSTVA
                     QLALQMGFDPIDCGSLPRARILESIGLLWARLAFEQGMGTDIAITLVRR"
     gene            1479..>3149
                     /locus_tag="VGB85_05165"
     CDS             1479..>3149
                     /locus_tag="VGB85_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133443.1"
                     /translation="MQRTTLRSFALLTGLGLLPACPANVATTDTDAESSGTTSAETTD
                     PQTDPTASSTPPTSTTQTTPPGETTEEPVTTTQETSSTDPGTTTDNQDTTAGDTEDTT
                     TGPVDNLCSRLGGADAGIPELVTTFLGKVLTDDRINGYFLNADVDAGNLHACVVKQLG
                     ELAGCAGVTYDCQNMKDAHAGLGISQADFGDFVEDFVAAYDEHAATHPELTPEDETAI
                     GDALGMMAGDIVEDPENNLTVYQRVGRKPNIKMMIGHPGEAGTFVDNVVNDITIASFF
                     TNTNHDRFNTCLTRQVSSIDGPVKYGQEVTAPDGIDPGVSADDPCKDMKTAHEGLTDD
                     MMEGITVEDFLALVMDLVTAMQTFMVSDADQTTILGVLGPMCDDIVAEPNTCPGNSKT
                     ELIEAVDLNLAIDNMAMNGAWDDKYNGMLDSMLCVDIAVADDGLNFVNDVRLKVGMDH
                     SYVGDVTIKVVSPDAKVLTALGRPSNEPPLADNGVPCCGYPANLSAMFPFTLTDAATI
                     TGKDMGKGLTDLKVICKDENPKIDPCQFKPYKGGGPGTAFSDFKGKSAV"
BASE COUNT          545 a         1274 c          924 g          406 t
ORIGIN      
        1 cgcccggctc gccgcgctgc acaacaacat ggccggcctc gccgtcgacg acggccgcct
       61 cgccgacgcc gtgcccgact accgcagcgc gctcgagggc tttcgcgccg ccgggcccga
      121 caacgagtac acgatcaccg tcgaggccaa cctcgccgag accctgctcg cgctcggcca
      181 ggccgacgag gcgctcgcgc tgcaacagca cgtcgtcgcc gtccgcgagc gcgagtccgc
      241 cggcgacccc gcggccgccg tcgcccgcgt gcaacaggcg cagatcgtcc gcgccctcgg
      301 gcgcccggag gaggccgtcg cgctcctcga ggccgcgctc gccatcctcg agtccgcccc
      361 cgtcgagcac cgctggcgcc gcggcctggg ccgctacacc ctcgcccgct gcctcgccga
      421 gctgcgccgg gaccggccgc gccagcagct gctcgccagc ctcgccgccg acgatctcgc
      481 cagcgacggc gattacttcg cccgccgcgc cctgctcgag ctgcgcacct ggctcacccc
      541 gtcccgctga gcttttcgac atgtcccgcg ggacatgtcc gtaaagctcg gatcggccgc
      601 acgcgccagc ccaggcggcc cccgctgcca cgcgcgtcac accaccggcc cattgtggcc
      661 tcacgccccc ataccggccg catctgacct gtggcacact gtcgccatgc cctccatcag
      721 catcctcggg actggacgca tggccaccac cctcgcccgt tgttggctgg agaagggcaa
      781 cagcgtgacc ctcgggtcgc gcaaccccga gcaggcgcgc acccgcatcg gcgcgctcgc
      841 cgagcagctg accgtgaccg acttcgccag cgcctgcgcc agcgccgagg tcgtcgcgct
      901 cgcggtcccg taccacgcgg ccggcgacgt catcgccgcg gccggcgacc tcgagggcaa
      961 gatcatcatc gacaccacca acccgtggag cccggacagc caccacctcg cggtcgccgg
     1021 cgacacctcc ggcgccgaag agatcgcgcg ccgcgctcgc ggggcccacg tcgtcaaggc
     1081 gctcaacggc atcttctgga agaacctcaa ggacccgacg tttagcggcc tgccggcgtc
     1141 gatgttctac tgcggcgacg acgacagctc caagagcacc gtcgcccagc tcgccctgca
     1201 gatgggcttc gacccgatcg actgcggctc gctgccccgc gcgcgcatcc tcgagtcgat
     1261 cggcctgctg tgggcgcgcc tcgccttcga gcagggcatg ggcaccgata tcgcgatcac
     1321 cctcgtgcgc cgctagcgac cgtccgcgaa cgctgcccac gcgcgcgcgc gctcgcgccc
     1381 atgtgcacgc gctttcgcga ttctctcctg ccacgagccc ggataacgtt gggtcgctgg
     1441 ccctctcgtc cctcttaccc accagactgt cgtcccctat gcaacgcacc accctgagat
     1501 ccttcgcgct cctcaccggc ctcggcttac tgcccgcctg tcctgccaac gtggcgacga
     1561 ccgacaccga cgccgagagc tccggcacca cgtcggcgga gacgaccgat ccgcagaccg
     1621 acccgaccgc cagctcgacc ccgccgacga gcaccaccca gacgacgccg cccggtgaga
     1681 cgaccgagga gccggtcacg accacgcagg agacctcctc gaccgacccc ggcacgacca
     1741 ccgataacca ggacaccacc gccggcgaca ccgaggacac gaccaccggc ccggtcgaca
     1801 acctgtgtag ccgcctcggt ggcgccgacg cgggcatccc ggagctggtc accaccttcc
     1861 tgggcaaggt cctcaccgac gacaggatca acggctactt cctcaacgcc gacgtcgacg
     1921 ccggcaacct ccacgcctgc gtcgtcaagc agctcggcga gctcgccggg tgcgccggcg
     1981 tcacctacga ctgccagaac atgaaggacg cccacgccgg gctcggcatc tcgcaggccg
     2041 acttcggcga cttcgtggag gacttcgtcg ccgcctacga cgagcacgcc gccacccacc
     2101 cggagctgac ccccgaggac gagaccgcga tcggcgacgc gctcggcatg atggccggcg
     2161 acatcgtcga ggaccccgag aacaacctga ccgtctatca acgcgtcggc cgcaagccga
     2221 acatcaagat gatgatcggg cacccgggcg aggccggcac cttcgtcgac aacgtcgtca
     2281 acgacatcac gatcgccagc ttcttcacga acaccaacca tgaccgcttc aacacctgcc
     2341 tgacccgcca ggtcagcagc atcgacggcc ccgtcaagta cggccaggag gtcaccgcgc
     2401 cggacggcat cgaccccggc gtctcggccg acgacccctg caaggacatg aagaccgccc
     2461 acgagggcct gaccgacgac atgatggagg gcatcacggt cgaggacttc ctcgcgctcg
     2521 tcatggacct cgtcaccgcg atgcagacct ttatggtctc cgacgccgac cagaccacga
     2581 tcctcggcgt gctcgggccg atgtgcgacg acatcgtcgc cgagcccaac acctgccccg
     2641 gcaactccaa gaccgagctg atcgaggccg tcgacctcaa cctcgccatc gacaacatgg
     2701 cgatgaacgg cgcctgggac gacaagtaca acggcatgct cgactcgatg ctctgcgtcg
     2761 acatcgccgt cgccgacgac ggcctcaact tcgtcaacga cgtgcgcctc aaggtcggta
     2821 tggaccacag ctacgtcggc gacgtgacga tcaaggtcgt ctcccccgac gccaaggtgc
     2881 tgaccgccct cggccgcccg agcaacgagc ccccgctcgc cgacaacggc gtgccctgct
     2941 gcggctaccc ggccaacctc agcgcgatgt tccccttcac cctcaccgac gccgcgacga
     3001 tcaccggcaa ggacatgggc aagggcctca ccgacctcaa ggtcatctgc aaggacgaga
     3061 acccgaagat cgacccgtgc cagttcaagc cctacaaggg cggcggcccc ggcacggcct
     3121 ttagcgactt caagggcaag agcgccgtc
//
LOCUS       DASWNJ010000285         4592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_392765, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000285 DASWNJ010000000
VERSION     DASWNJ010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392765"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(736..1089)
                     /gene="rplT"
                     /locus_tag="VGB85_05170"
     CDS             complement(736..1089)
                     /gene="rplT"
                     /locus_tag="VGB85_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEY0133444.1"
                     /translation="MPRAKRGFKARRRRNRILKAAKGFRGGRSKMFRRALEAVHHAWM
                     HMYTSRKGLKRVMRRLWIVRLNAAARIEGLSYSRLIEGIGKAGIALDRKVLSDLAIFD
                     PPGFGKVVAAARAAL"
     gene            complement(1093..1287)
                     /gene="rpmI"
                     /locus_tag="VGB85_05175"
     CDS             complement(1093..1287)
                     /gene="rpmI"
                     /locus_tag="VGB85_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEY0133445.1"
                     /translation="MKSHSGAKKRFRFTSTGKVKSKHSHKNHILTKKKSDRIRKLKGT
                     TVVSEPDCIRIHIMLPNGRP"
     gene            complement(1446..2363)
                     /gene="infC"
                     /locus_tag="VGB85_05180"
     CDS             complement(1446..2363)
                     /gene="infC"
                     /locus_tag="VGB85_05180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEY0133446.1"
                     /translation="MGAGPGCLTRARDRSHTVAPSGGSSHHSRSLPAAFSRRSTTIGR
                     RQSKPKPRVETHRVGRRIRVKEVRVIDDKGEQLGIMTTQEAMSRADELGLQLVEVNPK
                     TVPPVCKIMDYGKFKYETAKRDREAKKHQKTTELKEVKFRPKTHDHDFDFKTRHIRRF
                     IEEGDKVKLVVQFRGREITHPETGRKILEKVITELADVTLILQMAQMEGNRMNMVLGP
                     KPKRDMPKGAPAKPTGPAAPRPIQPPHPAAVAAAAAKAATAAAAAAAAAAAAPPDADT
                     DDEDDDDYDDDLIDDTTGLDDIVDPPTKQ"
     gene            complement(2649..4421)
                     /gene="thrS"
                     /locus_tag="VGB85_05185"
     CDS             complement(2649..4421)
                     /gene="thrS"
                     /locus_tag="VGB85_05185"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEY0133447.1"
                     /translation="MADVHLQEIEHMIDPSVPDLSPVEKMRHSAAHIMAAAIKKLWPE
                     ARLAFGPHTEDGFYYDIDMPHRLVPEDFERIEAAMQAEVDAKHPFVREEVTRAQAIEM
                     FTNWGETLKVEAIAAIPGDATLTVYRSGDFVDLCRGPHVTNTAQVRAFKIMSIAGAYW
                     RGDETRPQLQRVYGTAFNDRKELKEYLRMLEEAKARDHRKLGKELGLFHFDPIAPASP
                     FFTGRGTVVYNALQAYVRQLYRKHGYEEIITPQIFDMDLFKTSGHYDHYKDNMFACTI
                     DEREFGVKPMNCPGHCVLFRQTRRSYRDLPVRFADFGRLHRYERAGVTHGLTRVRSFA
                     QDDAHVFCTTDQVGDEIGKVTAMILECYKTFGFDQPEVEVSTRPDSFLGERETWDRAE
                     AALMASLDAAGLPYKINAGDGAFYGPKIDFKVRDALRRSWQLGTVQLDYQLPERFGLE
                     FVAPDDSRGRPVMIHRAMLGSLERFFGVYLEHVAGRFPTWIAPVQVAVLPITDAHHDW
                     THEVAAALQAAGLRVETDLRSEKLGLKIREATLLRVPYMLVLGDKEVEARGVAPRSRD
                     GKTGPLEPLADFVTRLTDEAKIPA"
     gene            complement(4535..4591)
                     /locus_tag="VGB85_05190"
     tRNA            complement(4535..4591)
                     /locus_tag="VGB85_05190"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4565..4567),aa:Val,seq:gac)
BASE COUNT          677 a         1520 c         1623 g          772 t
ORIGIN      
        1 cgggcgtacg atgtcgccag cccgcgccgt ctgctgtggg cgagctgtgg cggctcgcta
       61 catcacttcg ccagtgcagc gagcacccgg gccggcacga ctggccgctc agcagccgag
      121 cgccaaagca gcagcagcga gcagcaagcc agcacgacct cgccactcgg catgcggccc
      181 aaaagccagc cgagcaccga ggtagacgac ctcgccactc ggcatgcggc ccaaaagcca
      241 gcagccgagc acccgaagcg ggcgacctcg ccatttcagc agccgagcac ccgaagcggg
      301 gcgcagacga tgccacacgg aggggtgagg gccggtggcc cgggacccag gcgggggggc
      361 ccccggctcg cgcgcaggcg cctcgtgtgg tccttcgcgg ctgcgatgac cgcgaggatc
      421 gcgggtcatt cgtctcgaga tggcgccgag cacgtgacgg ggaggaaccg ccgtcgggct
      481 agggacccgc agccaccggc ccccctccgc ccatatcact gcacgccccg cgtccccgat
      541 ctcgccagct ccgcccgccg agccagcccg cccgcgaagc cagcgccgcc cgccgagcca
      601 gcccgcccgc cgagccagcc ccggccgccg agcccgcggc cccttccaag cgcccgcgaa
      661 gcccaaccaa cgaagcaacc gacgcccggc caaagcccag cgtcggtccc atcggagaaa
      721 agggtgaaga aaggactaca gggcagcccg ggcagccgcg acgaccttgc cgaagcccgg
      781 cgggtcgaag atggcgaggt ccgagagcac cttgcggtcg agggcgatgc cggccttgcc
      841 gatgccttcg atcaggcggc tgtagctgag cccctcgatg cgggcggccg cgttgaggcg
      901 gacgatccac aggcgacgca tgacgcgctt gaggcccttg cggctggtgt acatgtgcat
      961 ccacgcgtgg tgcacggcct ccagggcgcg acggaacatc ttggagcgac cgccccggaa
     1021 gcccttggcg gccttcagga tgcggttacg acgacggcgg gctttaaatc cacgttttgc
     1081 gcgaggcatg gttcacggcc tcccattcgg aagcatgatg tgaatacgga tgcaatccgg
     1141 ctcggagacg acggtcgtcc ccttgagctt gcggatgcgg tccgacttct tcttcgtcag
     1201 gatgtggttc ttgtgcgagt gcttgctctt cactttaccc gtggaggtaa agcgaaagcg
     1261 cttcttcgcg cccgaatggg acttcatctt tggcatggct tgtccgaagg gggggcttat
     1321 gtacctcagg cgtccgaggc aggcaagagc ctgggtggcc cgtaccacgg cgggtacggg
     1381 cccttccagg ccggatgtca cgatgtgttt ttgggttggc tcacacgccg aggcgtgtgc
     1441 tccggctatt gcttggtcgg cgggtcgacg atgtcgtcga ggccggtggt gtcgtcgatg
     1501 aggtcgtcgt cgtaatcgtc gtcgtcctcg tcatccgtat cggcgtccgg gggtgccgct
     1561 gcagctgcag ctgccgccgc cgctgccgcg gtggctgcct tggcggccgc cgctgcgacc
     1621 gccgccgggt gcggcggctg gatcggacgc ggggcggccg ggccggtcgg cttggccggg
     1681 gcgcccttgg gcatgtcacg cttgggcttg ggacccagga ccatgttcat gcggttgccc
     1741 tccatctgcg ccatttgcag gatgagggtc acgtcggcga gctcggtgat caccttctcg
     1801 aggatcttgc gaccggtctc ggggtgggtg atctcgcggc cgcggaactg gacgacgagc
     1861 ttgaccttgt cgccctcctc gatgaagcgc cgaatgtgcc gagtcttgaa gtcgaagtcg
     1921 tggtcgtgcg tcttcggccg gaacttgact tccttgagct cggtggtctt ctggtgcttc
     1981 ttggcctcgc ggtcgcgctt ggccgtctcg tacttgaact tcccgtagtc catgatcttg
     2041 cacacgggag gtacggtctt cgggttgact tcaaccagct gaagaccgag ctcatcggcg
     2101 cgggacatgg cttcctgggt ggtcatgatg ccaagctgct cacccttgtc gtcgatgacg
     2161 cgcacctcct tgactcggat gcggcgaccg acgcgatggg tctctacgcg cggcttgggt
     2221 ttggattgtc tgcggccgat ggtcgtgctc ctgcgagaga atgcggccgg cagggatcgc
     2281 gagtggtgcg aagacccgcc ggacggggcg acagtgtgcg agcgatcgcg ggcgcgcgtc
     2341 aagcagcctg gacccgctcc cacatgtttt ctcgaccacc gccccgctct atcgggcctt
     2401 tacggggcag atcttccccg aggcgccgga gatccgccct gcccggctcc tgaccccttc
     2461 cgagggatcg cgagttccag cggaccccgg ttgcttgccg ggatcggcac cacgggcggc
     2521 ccgcggatgt gcgctcgcgc gtccgctgtc atcgcaggcg atgcgatgtc ccacgccgca
     2581 ccagccggag ccggtccgag ctcgccacag caccacgagc cgcgccgcgc gggcccggcg
     2641 ggcgccgctc aggcggggat cttcgcctcg tcggtgagcc gcgtgacgaa gtcggcgagc
     2701 ggctcgagcg ggccggtctt gccgtcgcgg ctgcgcgggg cgacgccgcg ggcctcgacc
     2761 tccttgtcgc cgagcacgag catgtacggg acgcgcagca gggtcgcctc gcggatcttg
     2821 aggccgagct tctcgctgcg caggtcggtc tcgacgcgca aacccgcggc ctggagggcc
     2881 gcggcgacct cgtgggtcca gtcgtggtgg gcgtcggtga tcggcaggac cgcgacctgg
     2941 accggggcga tccaggtcgg gaagcggccg gcgacgtgct cgaggtagac gccgaagaag
     3001 cgctcgagcg atccgagcat cgcgcggtgg atcatcaccg ggcggccgcg cgagtcgtca
     3061 ggggcgacga actcgaggcc gaagcgttcg ggcagctggt agtcgagctg cacggtgccg
     3121 agctgccacg agcggcgcag ggcgtcgcgc accttgaagt cgatcttcgg gccgtagaag
     3181 gcgccgtcgc cggcgttgat cttgtacggg aggccggccg cgtcgagcga ggccatcagg
     3241 gccgcctcgg cgcggtccca ggtctcgcgc tcgccgagga acgagtccgg gcgggtgctg
     3301 acctcgacct cgggctggtc gaacccgaag gtcttgtagc actcgaggat catcgcggtg
     3361 accttgccga tctcgtcgcc gacctggtcc gtcgtgcaga acacgtgggc gtcgtcctgc
     3421 gcgaagctgc ggacgcgcgt caggccgtgg gtcacgccgg cgcgctcgta gcggtgcagg
     3481 cggccgaagt cggcgaagcg caccggcagg tcgcggtagc tgcggcgggt ctggcggaac
     3541 aacacgcagt gaccggggca gttcatcggc ttgacgccga attcccgctc gtcgatcgtg
     3601 cacgcgaaca tgttgtcctt gtagtgatcg tagtggcccg aggtcttgaa cagatccatg
     3661 tcgaagatct gcggggtgat gatctcctcg tagccgtgct tgcggtacag ctggcggacg
     3721 taggcctgca gcgcgttgta gacgaccgtg ccgcggccgg tgaagaacgg gctcgccggc
     3781 gcgatcgggt cgaagtggaa caggccgagc tccttgccga gcttgcggtg gtcgcgggcc
     3841 ttggcctcct cgagcatccg caggtactcc ttgagctcct tgcggtcgtt gaaggccgtg
     3901 ccgtacacgc gctgcagctg cgggcgcgtc tcgtcgccgc gccagtaggc cccggcgatg
     3961 ctcatgatct tgaacgcccg cacctgcgcg gtgttggtga cgtgcggccc gcggcacagg
     4021 tcgacgaagt cgccgctgcg atagaccgtc agcgtcgcgt cgcccgggat cgccgcgatc
     4081 gcctcgacct tgagcgtctc gccccagttg gtgaacatct cgatcgcctg cgcgcgcgtg
     4141 acctcctcgc gcacgaaggg gtgcttggcg tcgacctcgg cctgcatcgc agcctcgatg
     4201 cgctcgaagt cctccggcac caggcggtgc ggcatgtcga tgtcgtaata aaagccgtcc
     4261 tcggtgtgcg gaccgaacgc cagccgggcc tccggccaca gcttcttgat cgcggccgcc
     4321 atgatgtgcg ccgcgctgtg gcgcatcttc tccacgggcg acaggtccgg gacggacggg
     4381 tcgatcatgt gctcaatctc ttgcaggtgt acgtcggcca tggcgcccgc agggtacacg
     4441 aggcgacccg gcccgcgcga gcaacacgga cagcatgaac aagaagaccc gccgcggcgg
     4501 ggccgggcgg gtcttctcag ctgcttgtag gactgagggg tttcgaaccc ctgaccccta
     4561 ccgtgtcaag gtagtgctct accactgagc ta
//
LOCUS       DASWNJ010000286         2835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_392793, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000286 DASWNJ010000000
VERSION     DASWNJ010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392793"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VGB85_05195"
     CDS             complement(<1..398)
                     /locus_tag="VGB85_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133448.1"
                     /translation="MRRAAHMSMWWGPVVLVGALLSGACGDDGIAMTEGVTESGTSSG
                     TTDTPTTENPTTPTTTDPTETTETPTTTPPTTGDTSTTSPVTSSTTDETTGTESDTDT
                     TGLTTTGNTTGDTDGTTGTTTGDTTTGDTDT"
     gene            777..1433
                     /locus_tag="VGB85_05200"
     CDS             777..1433
                     /locus_tag="VGB85_05200"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0133449.1"
                     /translation="MSTASLACDGQAPTPKAAPPQTSTAANPGPGTPAPDAADADKHY
                     QSALTLEAAGRYVEARTEVDLAIAGGAGKDAKLLAAKLAILRDDLDAARRLLEPLADS
                     NDALVLYNLGLVAQRTGAYNEARTRYLAALKADPNYAAIRYNLAVLTWDAGVKDEAQH
                     HARKFLEMSPGDPNGAELRRKVELDGPPATDTSGEGKADTKADTKADTKALRKPAAPR
                     "
     gene            complement(1408..>2835)
                     /locus_tag="VGB85_05205"
     CDS             complement(1408..>2835)
                     /locus_tag="VGB85_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HEY0133450.1"
                     /translation="GAYLSLDEVTITLAIAAYGFVASVLPVWMLLCPRDYLSSYMKIG
                     TILLLIAGIMIVNPVIEMPMINEVGPGGIGVDGEQFPAVVRGSLFPFVFITIACGAIS
                     GFHALIASGTTPKMVDRETDCRPIGYGAMLMEGLVGITALLAATALPPADYFAINTDP
                     EIAVVAGADSHGLARDRAELTRLDEVLTPEDRQRLGLGEQQPASAAAGGKIKVSEALR
                     LSNGTLAALGYHADPADAHANELTTADFARMGIRVEDLPTLAEATDEVVAARTGGAVS
                     LAVGMARVFSGLPGMRALLDYWYHFAIMFEALFILTTIDTGTRVGRFLLQEALGRRWP
                     RLGDTSWPPAAALSSLVIVVGWSYFILTGSIATIWPMFGIANQLLATVALAVGSTILL
                     REARRPAYAWVTLAPLMFVAGTTITAAVRSLFEVYVPMAMVPATATKGAINAGVTGTL
                     LICVLAILTMSARRWQEIIAARRAS"
BASE COUNT          439 a         1088 c          878 g          430 t
ORIGIN      
        1 gtgtccgtgt cacccgtcgt cgtgtccccg gtcgtcgtcc cggtcgtacc gtccgtgtca
       61 cccgtcgtgt ttcccgtcgt cgtcagcccc gtcgtgtcgg tgtcgctctc cgtccccgtc
      121 gtctcgtccg tcgtcgagga cgtgaccggc gaggtcgtcg aggtgtcgcc cgtcgtcggc
      181 ggcgtcgtcg tgggtgtctc cgtcgtctcc gttggatccg tcgtcgtcgg cgtcgtgggg
      241 ttctccgtgg tcggcgtgtc cgtcgtcccc gaagatgtcc cgctctccgt cacaccctcc
      301 gtcatggcga tcccgtcgtc gccacacgcc ccgctcagca gcgctcccac cagcacgacc
      361 ggaccccacc acatgctcat gtgtgcggct cggcgcatgt tatccactcc tgtttgttgt
      421 ccccttggca tcaaaaacca gtcgacacga cgaccctatc aacaagcacc aatcaccaca
      481 accgatgccc tggctctcga gacaggtgaa gatcgctggc ggcgctccgc tcgctgatcg
      541 tcctcgactg aggagaagat cctgcacctg cggacgtctc tatacatgtc cgatggaaca
      601 tgtcctgcac gccatgtcga tcacgagaca cgcctcgtcc gcgccagatc gcccgctgcc
      661 tcggcgcttg cgacgccggg ttccgatggc cgccgctgtg cccgacgtgg tagaaacgtg
      721 ctccgaggtc cgccttgcca tctcagtccc gtctcgtctc gtcgctgctc atccttgtgt
      781 ccaccgcgtc gctcgcttgc gacggtcaag ctccgacgcc gaaggctgcg cctccgcaga
      841 cgtcgaccgc ggcgaaccct ggcccgggta cgccggcccc cgacgctgcc gacgccgaca
      901 agcactacca gagcgcgctc acgctcgagg ccgcgggtcg ttatgtggag gcccgcaccg
      961 aggtcgacct ggcgatcgcc ggcggcgccg gcaaggacgc caagctgctc gccgccaagc
     1021 tggcgatcct gcgcgacgat ctcgacgccg cgcggcgcct gctcgagccg ctcgccgaca
     1081 gcaacgacgc gctcgtcctc tacaacctcg gcctcgtcgc ccagcgcacc ggcgcataca
     1141 acgaggcgcg cacgcgctac ctcgccgccc tcaaggccga cccgaactac gcggcgatcc
     1201 gctacaacct cgcggtgctg acctgggacg ccggcgtcaa ggacgaggcc cagcaccacg
     1261 cgcgcaagtt cctcgagatg tcacccggcg acccgaacgg cgccgagctg cgccgcaagg
     1321 tcgagctcga cggcccgccc gcgaccgaca ccagcggcga gggaaaggcc gacaccaagg
     1381 ccgacaccaa ggccgacacc aaggccctca ggaagcccgc cgcgccgcga tgatctcctg
     1441 ccaccggcgc gcgctcatcg tcaggatcgc cagcacgcag atcagcagcg tgcccgtgac
     1501 ccccgcgttg atcgctccct tggtcgcggt cgccgggacc atcgccatcg gcacgtagac
     1561 ctcgaacagc gagcgcaccg cggccgtgat cgtcgtgccc gccacgaaca tcagcggcgc
     1621 cagggtgacc cacgcgtacg ccggccgcct cgcctcgcgc agcaggatcg tcgagccgac
     1681 cgccagcgcc accgtcgcga gcagctggtt ggcgatgccg aacatcggcc aaatggtcgc
     1741 gatcgatccg gtcaggatga agtagctcca cccgaccacg atcaccagtg acgacagcgc
     1801 ggccgccggc ggccaggacg tgtcgccgag ccggggccag cgccgcccga gggcctcctg
     1861 caacaggaac cgaccgaccc gcgtcccggt gtcgatcgtc gtcaggatga acagcgcctc
     1921 gaacatgatc gcgaagtggt accagtagtc gagcagcgcc cgcatgcccg gcaagcccga
     1981 gaacacgcgc gccatgccga ccgccagcga caccgccccg cccgtgcgcg ccgccaccac
     2041 ctcgtcggtc gcctccgcca gcgtcggcag gtcctcgacc cggatcccca tccgtgcgaa
     2101 gtctgcggtc gtcagctcgt tggcgtgggc gtcggccggg tcggcgtggt agccgagcgc
     2161 cgcgagcgtg ccgttcgaca gccgcagcgc ctccgacact ttgatcttgc cgcccgccgc
     2221 cgcgctcgca ggttgctgtt cgccgagccc cagccgctgg cggtcctccg gcgtcagcac
     2281 ctcgtcgagg cgagtcagct cggcccggtc gcgcgcgagc ccgtggctgt ccgcccctgc
     2341 gacgaccgcg atctccgggt ccgtattgat ggcaaagtag tccgcgggcg gcagcgccgt
     2401 cgcggcgagc agcgccgtga tcccaaccag gccctccatc agcatcgccc cgtagccgat
     2461 cggccggcag tcggtctcgc ggtcgaccat cttcggcgtc gtgccgctgg cgatcagcgc
     2521 gtggaacccc gagatcgcgc cgcacgcgat cgtgatgaac acgaagggga acagcgagcc
     2581 gcgcaccacc gctggaaatt gctccccgtc gaccccgatc ccgccgggcc cgacctcgtt
     2641 gatcatcggc atctcgatca ccgggttgac gatcatgatc ccggcgatca acagcaggat
     2701 cgtgccgatc ttcatgtacg agctgaggta gtcgcgcggg cacagcagca tccacaccgg
     2761 gagcacgctg gcgacgaagc cgtacgccgc gatcgccagg gtgatcgtca cctcgtcgag
     2821 gctgaggtac gcccc
//
LOCUS       DASWNJ010000287         3144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_675891, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000287 DASWNJ010000000
VERSION     DASWNJ010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675891"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VGB85_05210"
     CDS             <1..291
                     /locus_tag="VGB85_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0133451.1"
                     /translation="VIDLRTLRPLDTDAIIDSVKKTNRAVVLFHDWPYGGVGCEIIAR
                     IQELAFDHLDAPVLRECYEDVPFPYAENLEQLCLPTPEKGLAAVKKVLYKRG"
     gene            355..1773
                     /locus_tag="VGB85_05215"
     CDS             355..1773
                     /locus_tag="VGB85_05215"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEY0133452.1"
                     /translation="MVTLPRLSDTMEEGVIAKWRIKVGDKVKRGQVIAEIETDKATME
                     FESFDSGTVLKLVAGEGEALPVGATIAVLGQPGENPDAGGKTSEAKASKPVEGTTPIT
                     GTAPPEGANGEAKLADPAGDQAKADVRADAEPASSPEGQGAKDMPQKPAARDDGRVPA
                     SPLARRLAREHDLEISQIPGTGPHGRVVKTDVEQAIAASGKAPAKAAATAAASEPAAP
                     AAQRAPAGGAAATFSSEADVDGRPYATRANRTIKLSQMRKTIVKRMGQSKREAPHIYL
                     TTAIHMDRAMAVRGELNAALEGTKISVNDLIVLACARALRKHPKVNSNYTEAGIVEHG
                     DIHVGVAVALDDGLVVPVIRFADQKSISAISRETRDLGNRAKNKELRPEEMTGSTFTI
                     SNLGMLGVESFMAVVNPGESAILAVGATNEEPVVEAGAVVVRKVMRVTLCSDHRAFDG
                     ADSARFLQTLKSLLEAPMALFG"
     gene            complement(1847..2782)
                     /locus_tag="VGB85_05220"
     CDS             complement(1847..2782)
                     /locus_tag="VGB85_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133453.1"
                     /translation="MPSSVAIACTLSLALVQDPRPPVDRPPEAAAAQEFGRPGTEAPA
                     DTTTPTDTAAGGTVEGPAEGPVSDAEVAANSQSDRTETNAVAPPRWKGNGLLITSGVL
                     GAIGLGANIGRIAVVQKLCREVAYDPDSDAIAGTERCQNEGAGLILLGAGAVAFNMAA
                     IGTGAAGGSLHGSWAAHDTAVRNGRMRAAGAQIGVGAGLLAIGVLGYVAVRVASFADA
                     LGANSCGEKYPVDPMAADQANSELTNCIRNRWSGYLGGITATQAAAVAGTGMLAHGVS
                     YSRNLKLYRTITSHQVNLRPIFAPTWTGVGLSARF"
BASE COUNT          508 a         1095 c         1146 g          395 t
ORIGIN      
        1 gtgatcgacc ttcgcacgct gcgcccgctc gacaccgatg cgatcatcga cagcgtcaag
       61 aagaccaacc gcgcggtggt gctgttccac gactggccct acggcggcgt cggctgcgag
      121 atcatcgccc gcattcaaga gctcgccttc gaccacctcg acgcgcccgt cttgcgcgag
      181 tgctacgagg acgtgccctt cccctacgcc gagaacctcg agcaactgtg cctgccgacc
      241 cccgagaagg gcctcgcggc ggtcaagaag gtgctctaca agcggggcta gtcagcgccc
      301 gcgtctcgtt tcttccgcag cagcagcagt aaggacagca ccgccatggc aaccgtggtt
      361 acactccccc gcctcagcga cacgatggaa gagggcgtga tcgccaagtg gcggatcaag
      421 gtcggcgaca aggtcaagcg cggccaggtc atcgccgaga tcgagaccga caaggcgacg
      481 atggagttcg agtcgttcga ctcgggcacc gtgctcaagc tcgtcgccgg cgagggcgag
      541 gccctgccgg tcggcgcgac gatcgcggtg ctcggccagc cgggcgagaa cccggacgcg
      601 ggcggcaaga ccagcgaggc gaaggcgagc aagccggtcg agggcacgac cccgatcacc
      661 ggcacggccc cgcccgaggg cgccaacggc gaggcgaagc tcgccgaccc ggcgggcgac
      721 caggccaagg ccgacgtccg cgccgacgcg gagccggcga gcagtcccga gggacagggc
      781 gcaaaggaca tgccccaaaa gccagccgca cgcgacgacg ggcgggtccc ggcctcgccg
      841 ctggcgcgcc gactcgcgcg cgagcacgac ctcgagatct cgcagatccc cggcacgggc
      901 ccgcacggcc gcgtcgtcaa gacggacgtc gagcaggcga tcgcggcgtc gggcaaggcc
      961 ccggcgaagg cggctgcgac cgcggccgcg agcgagccgg cggccccggc agcccagcgg
     1021 gccccggccg gcggcgccgc ggcgaccttc tcgagcgagg ccgacgtcga cgggcgcccg
     1081 tacgcgacgc gcgcgaaccg gacgatcaag ctcagccaga tgcgcaagac gatcgtcaag
     1141 cgcatgggcc agagcaagcg cgaagcgccg cacatctacc tgacgacggc gatccacatg
     1201 gaccgcgcga tggcggtccg cggcgagctc aacgcggcgc tcgagggcac caagatcagc
     1261 gtcaacgacc tgatcgtgct cgcctgcgcc cgcgcgctgc gcaagcaccc gaaggtcaac
     1321 tcgaactaca ccgaggccgg catcgtcgag cacggcgaca tccacgtcgg cgtcgcggtg
     1381 gcgctcgacg acggcctcgt cgtgccggtg atccgcttcg cggaccagaa gtcgatctcg
     1441 gcgatctcgc gggagacgcg ggacctcggc aaccgggcca agaacaagga gctgcggccc
     1501 gaggagatga ccggcagcac cttcacgatc agcaacctcg gcatgctcgg cgtcgagagc
     1561 ttcatggcgg tcgtcaaccc gggcgagagc gcgatcctgg cggtcggcgc gaccaacgag
     1621 gagcccgtcg tcgaggccgg cgccgtggtc gtccgcaagg tcatgcgcgt caccctgtgc
     1681 tcggaccacc gcgccttcga cggagccgac agcgcccgct tcctgcagac gctcaagagc
     1741 ctgctcgagg ccccgatggc gctgttcggc taacagcgcc cggatgcact cgccggtggt
     1801 gaccggcggg tgcagggccg gcggctcgcc agcgccacac gtcgactcag aagcgggccg
     1861 agaggccgac gccggtccag gtcggcgcga agatcgggcg caggttgacc tggtggctgg
     1921 tgatcgtgcg gtacagcttg aggttgcggc tgtagctgac gccgtgggcg agcatgccgg
     1981 tgccggcgac ggcggcggcc tgcgtggccg tgatgccgcc gaggtagccg ctccagcggt
     2041 tgcggatgca attggtcagc tccgagttgg cctggtcggc ggccatcggg tcgaccgggt
     2101 acttctcgcc acagctgttg gctccgagcg cgtcggcgaa ggaggcgacg cggacggcga
     2161 cgtagccgag cacgccgatg gcgagcaggc cggctccgac gccgatctgg gccccggccg
     2221 cgcgcatgcg gccgttgcgg acggccgtgt catgcgcggc ccagctgccg tgcagcgagc
     2281 cgccggcggc tccggtcccg atcgcggcca tgttgaaggc gaccgcgccg gcgccgagca
     2341 ggatcaggcc ggcgccctcg ttctggcagc gctcggtgcc ggcgatcgcg tccgagtcgg
     2401 ggtcgtacgc gacctccctg cacagcttct gcacgacggc gatgcgcccg atgttggccc
     2461 cgaggccgat cgcaccgagc acgccagagg tgatcagcag gccgttgccc ttccaccgcg
     2521 gcggcgcgac ggcgttggtc tcggtccggt cactctgcga attcgcggcg acctcggcgt
     2581 cgctcacggg cccctcggca ggcccctcga cggtgccccc tgccgcggtg tcggtcggcg
     2641 tcgtggtgtc ggcgggcgcc tccgtcccgg gacggccgaa ctcctgggcc gcggcggcct
     2701 cgggcggacg gtcgacgggc gggcgcgggt cctggacgag ggcaagcgag agggtgcagg
     2761 cgatggcgac gctcgagggc attgcgggct ccggtgggaa aaaagatgaa gcgttgcgac
     2821 gcaggtcggc ggctcgccga tcgacgcgtc gatgctggaa tcgagagtca cgaggcgcca
     2881 cgggtcgccc ggtcgccaaa ctcattcacg aacgcttcac gtaccggcgc gagactcatg
     2941 ttcgcgccag gcgaaccatg tgcctgtggc ccacggatgc aacccgcgag ggcgatcgat
     3001 gcgacgatca tcggcgatgg cgccgctgct ggcaccacgc gtcgagatgt tgcgagccgg
     3061 cgcggatggc gaagctcgca tgagcgcatg cgtctgcatg ctcccgggcc acgctggagc
     3121 acagcgattc ggcagcctgg ggtc
//
LOCUS       DASWNJ010000288         1790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_675926, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000288 DASWNJ010000000
VERSION     DASWNJ010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675926"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1790)
                     /locus_tag="VGB85_05225"
     CDS             complement(<1..>1790)
                     /locus_tag="VGB85_05225"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY0133454.1"
                     /translation="LIDRPLRPSFTKGLRNEVQVVETVLALDPQHPYDVVAMNGASMS
                     TKLSGLPFSGPVGSTRVAHIDGQWVAFPTIEELERATFDMVVAGRVTADGDVAIMMVE
                     AEATPQALSLIAAGAPKPTEEVVASGLEAAKPAIRELCRAQSELAEVAAKPVLEYPVF
                     LDYQDDVYAAVSDAAKGELAEALKIAGKADREEALDRVKAKTVELVGPQFEGREKEIS
                     AAFRSVTKAEVRQRVLRDQVRMDGRGPRDIRPLSAEVGVLPRVHGSALFERGETQILG
                     VTTLNMLRLEQSLDTLSPDKSKRYMHNYNFPPYSTGETGRVGSPKRREIGHGALAERA
                     LVPVLPTREEFPYAIRQVSEALGSNGSTSMGSVCASTLALLSAGVPLKAPVAGIAMGL
                     ISDEVDGKTEYVTLTDILGAEDAFGDMDFKVAGTLDFVTALQLDTKLDGIPSDVLAAA
                     LQQAHDARTTILGVMDQAINSPAEMSEYAPRVTSVKIPVDKIGMVIGPKGQTINAIQD
                     ETGADISIEDDGTIYVGATNGPAAEAAVERINAIANPTMPKAGDKFLGTVVKTAAFGA
                     FISLLPGRDGLLHISKVGDGKRVEKVEDFL"
BASE COUNT          237 a          619 c          645 g          289 t
ORIGIN      
        1 ttgaggaagt cctcgacctt ctcgacccgc ttgccgtcgc ccaccttgga gatgtgcagc
       61 aggccgtcgc ggcccggcag cagcgagatg aacgcgccga acgcggccgt cttcaccacc
      121 gtgccgagga acttgtcccc cgccttgggc atcgtcgggt tggcgatggc gttgatccgc
      181 tcgaccgcgg cctcggccgc cgggccgttg gtcgcgccga cgtagatcgt gccgtcgtcc
      241 tcgatcgaga tgtccgcgcc ggtctcgtcc tggatcgcgt tgatggtctg gcccttcggg
      301 ccgatgacca tgccgatctt gtcgaccggg atcttgacgc tggtgacccg cggggcgtac
      361 tcgctcatct cggccgggct gttgatggcc tggtccatga cgccgaggat cgtggtacgg
      421 gcgtcgtggg cctgctgcag agcggcggcc agcacgtccg acgggatgcc gtcgagcttg
      481 gtgtcgagct gcagcgcggt gacgaagtcc agggtgccgg cgaccttgaa gtccatgtcg
      541 ccgaacgcgt cctcggcgcc gaggatgtcg gtcagcgtca cgtactccgt cttgccgtcg
      601 acctcgtcgg agatgaggcc catggcgatg ccggcgaccg gcgccttcag cggcacaccg
      661 gcgctgagca gggccagcgt cgaggcgcag accgaaccca tcgaggtgga gccgttggag
      721 cccagggcct cggagacctg gcggatcgcg tacgggaact cctcgcgcgt cggcagcacc
      781 ggcaccaggg cccgctcggc gagcgcgccg tggccgatct cgcgccgctt cggcgaaccg
      841 acccggccgg tctcaccggt cgagtacggc gggaagttgt agttgtgcat gtagcgcttg
      901 gacttgtccg gggacagcgt gtccagggac tgctccagcc gcagcatgtt cagcgtggtg
      961 acgcccagga tctgggtctc gccccgctcg aacagcgccg agccgtgcac ccgcggcagc
     1021 acgccgacct cggcggacag cggccggatg tcgcgcgggc cccggccgtc catgcggacc
     1081 tggtcgcgga gcacgcgctg gcgcacctcg gccttggtga ccgagcggaa cgccgcgctg
     1141 atctccttct cgcgaccctc gaactgcggg ccgacgagct cgacggtctt ggccttgacc
     1201 cggtcgaggg cctcctcgcg gtcggccttg ccggcgatct tcagcgcctc ggccagctcg
     1261 cccttggcgg cgtccgagac ggcggcgtac acgtcgtcct ggtagtccag gaagaccggg
     1321 tactccagca cgggcttggc ggcgacctcg gccagctcgc tctgcgcgcg gcacagctcg
     1381 cggatggccg gcttggcggc ctcgagcccg ctggccacga cctcctcggt cggcttcggc
     1441 gcgccggccg cgatcaggct cagcgcctgc ggggtggcct cggcctcgac catcatgatc
     1501 gcgacatcgc cgtcggcggt cacgcgaccg gcgacgacca tgtcgaaggt ggcccgctcg
     1561 agctcctcga tggtcgggaa ggcaacccac tggccgtcga tgtgcgcgac ccgggtcgag
     1621 ccgaccgggc cgctgaacgg caggccggag agcttggtcg acatcgaggc gccgttcatg
     1681 gcgacgacgt cgtacgggtg ctgcgggtcg agcgcgagga cggtctcgac gacctggacc
     1741 tcgttgcgca ggcccttggt gaacgacggg cgcagcggcc ggtcgatcag
//
LOCUS       DASWNJ010000289         5906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_111466, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000289 DASWNJ010000000
VERSION     DASWNJ010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111466"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VGB85_05230"
     CDS             complement(<1..904)
                     /locus_tag="VGB85_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133455.1"
                     /translation="MREVTGTTRTTTGPVRFRVVGMEDGMMLRQLLGKRLRLPPAAAS
                     EIVRAGGAYVGHIRICVPTQRVSEGERVTVYPEAARISAVEPAALHLLHRDDNCVIVD
                     KPHGVPAAATKAANRGTLAQALVARLTQEGVLRPYVGLVHELPAAASGLALYTIRSQA
                     TGSFFKEFAALPVQRGYRVRLRGSFPGDAIACAAEVVQLPAGGGWRLARAGDREPIAA
                     RTEFRKLVEVGEETLAAVELGSSPSQAIRLHAAALGLPVVGDGEPDATLCLLAETLAF
                     TQPRTGAAISVRATLPAWAALAGEA"
     gene            complement(916..1815)
                     /locus_tag="VGB85_05235"
     CDS             complement(916..1815)
                     /locus_tag="VGB85_05235"
                     /inference="COORDINATES: protein motif:HMM:NF013589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEY0133456.1"
                     /translation="MDQLQRAIKTDFSLRVIAAVTTGLVAEASARHALRGIEVVALGR
                     ALTAACLLTTLTKHESERLRVEFRSSGPLRGLLIDARGDGTVRGCLQRHLASEEHTLL
                     SSGTGRLAVAPALGAHGTLIVTRDVGLEQRYQGSVEVMSGEVDRDLEHYLNTSEQLPS
                     VLACEVVLDHQGNVARAAGLLCQTFPGGDTTELDALRATLASGNLADLLRQDRSTADI
                     IGFALAGEPFDDMGSSPLQFKCNCGPDRARSVVSTLGAEDIEALATEQQQTEIRCEYC
                     GLVYVLTAEDLRALAADIRRERS"
     gene            complement(1871..3037)
                     /locus_tag="VGB85_05240"
     CDS             complement(1871..3037)
                     /locus_tag="VGB85_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY0133457.1"
                     /translation="MLALAGCLLLLPGAAECSTVAPPVPRAPEIAHAAVPEAVPVPTV
                     VAPATPPTTPTTRPPSPGARLEAEQNVIDVFRAAAPATVFVTQKRMVRDLSMRALEIP
                     AGTGTGFLWDAQGHVVTNYHVVDSGRAQGRYSVTLHSQKTYDAEFLGGDPNKDVAVLK
                     LIGCNEPLTPLPLLPPDVKLAVGQTAIAIGNPFGLDHTLTTGVISALGREVQGYGGVT
                     IRDMIQTDASINPGNSGGPLLDSGGYLIGMNTMIFSKTGSSAGIGFAVPVSAVRRVVP
                     QVIATGRVQHPGIGIEPLPDHYAARAGIKGVVVTRVSPGSPAQLAGIRGLVQDRRGEI
                     SLGDIIVGIDEHVVADFDDLYNAFDRFEVGDEVTVKLVRDGQAASVKVKLVDLP"
     gene            3243..5261
                     /locus_tag="VGB85_05245"
     CDS             3243..5261
                     /locus_tag="VGB85_05245"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0133458.1"
                     /translation="MNGAAPAPSLGPCPHCGQPHAEDVLVCPQESKVMPLEGRMLDNK
                     FRFTKLLGAGGMGAVWRAQNIRVRKAVAIKLMHPEFAGNPGILDRFRNEATAAGQIGS
                     QHICDIYDFGASVLGPYIVLEMLNGKSFGELIQQYGRVDPGLAAIIMRQALTGLEAAH
                     MVGIVHRDLKPENIFLHEPSPGRLLVKLMDFGISKFSQGGNDGKTGVGVLMGTPEYMS
                     PEQAEGAAGVDQRTDIWAMGAILYKALTGVEAFGGATMAATLVALSTKEPKPIESFAP
                     HVPAEMIAVVRKCLAKDPADRYQSARELSDALAPFEQLTGSIPMSAPQSSVTGLATTA
                     PQGTSAPLQAPTGTLTASSSGPATFITPHGPPSQFGGPSGGFAPTHSPAGNPSTFVGG
                     TLGAAGHTDDSWSIGAHAPETRPPTISGREGGSKIPLVILGVLLLVAIGGTGYFLTSG
                     SPKPGNIDGPQIEPATTAGAVAATTAGTPAGTTAGTPTTAGTTPDPTAGATAGTTAGT
                     PTGTTAGTTAGTTAGTTAGTDPTPGTTAGTTAGATPDPTAGDDDDDNKPTKLPPPKPD
                     LSLVSQEGALFTPKAPSASVATVDEAKAACSALKSKKFAKLTQWRLASSGEVLKFKGH
                     TDVQKLAYWVTDPGSAGRAKRVALVTGSVNEGDPASFKSTRPFCVTKR"
     gene            5326..>5906
                     /locus_tag="VGB85_05250"
     CDS             5326..>5906
                     /locus_tag="VGB85_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133459.1"
                     /translation="MTYDMRDLSFLACTLGAALATGCGDNGGGTSTETGTSTSSADTG
                     STDATQGPTDPGTTADVPTTDPTTTDSTTSLTTDPTSTTEVTSTTDPGTTTDDTTTGM
                     PACPYTPVDGTPGVGLEPVASGFDRPVLTVSHPKQPDRLFIVEQGGHIKILEPGMTMA
                     PPDSDDFLYVKVKNENANEIGAEQGLLGFAFHP"
BASE COUNT          889 a         2336 c         1916 g          765 t
ORIGIN      
        1 tcgcctcgcc ggcgagcgcg gcccaggccg gcagcgtcgc ccgcaccgag atcgccgcgc
       61 cggtgcgcgg ctgcgtgaac gcgagcgtct cggccagcag gcacagcgtc gcatccggct
      121 cgccgtcgcc gacgaccggc agccccaggg ccgccgcgtg cagccggatc gcctggctcg
      181 ggctgctccc gagctcgacg gcggccagcg tctcctcgcc gacctccacg agcttgcgaa
      241 actccgtgcg cgctgcgatc ggctcgcggt cacccgcgcg ggcgagccgc cagcccccgc
      301 cggcgggcag ctggaccacc tcggccgcgc acgcgatcgc atcgccggga aaactcccgc
      361 gcaggcgcac gcgatagccc cgctggaccg gcagcgccgc gaactcttta aaaaagctcc
      421 cggtcgcctg gctgcggatc gtgtacagcg ccaggccgct ggccgccgcc ggcagctcgt
      481 ggaccaggcc gacgtacggc cgcagcaccc cctcctgcgt cagtcgggcc accagcgcct
      541 gcgcgagcgt gccgcggttg gccgccttgg tggcagccgc cggcaccccg tgcggcttgt
      601 cgacgatcac gcagttgtcg tcgcggtgca gcaggtgcag cgccgccggc tcgacggccg
      661 agatcctcgc cgcctccggg tacaccgtca cgcgctcgcc ctcgctgacg cgctgcgtcg
      721 gcacgcagat ccggatgtgc ccgacgtagg ctccgcccgc gcggacgatc tccgaggcgg
      781 ccgcgggcgg cagccggagc cgcttgccga gcagctggcg cagcatcatg ccgtcctcca
      841 tgccgacgac gcgaaagcgc accggccccg tcgtcgtccg cgtcgtcccg gtcacctcgc
      901 gcatcgcttc accgctcacg agcgctcgcg ccggatgtcc gccgcgagcg cgcgcaggtc
      961 ctccgcggtc agcacgtaga ccagcccgca gtactcgcag cggatctccg tctgctgctg
     1021 ctccgtggcc agcgcctcga tgtcctccgc gccgagcgtc gacaccaccg agcgcgcgcg
     1081 gtcgggcccg cagttgcact tgaactgcag cggcgagctg cccatgtcgt cgaacggctc
     1141 accggcgagc gcgaacccga tgatgtccgc cgtgctgcga tcttgccgca gtaaatcagc
     1201 gaggttgccc gaagccagcg tcgcgcgcag cgcgtcgagc tcggtcgtat caccacccgg
     1261 aaaggtctgg cacagcagtc cggccgcgcg cgccacgttg ccttgatggt cgagcaccac
     1321 ctcgcaggcg agcacgctcg gcagctgctc gctggtattg agatagtgct cgaggtcgcg
     1381 gtcgacctcg ccggacatca cctcgacgct gccctgatag cgctgctcga gcccgacatc
     1441 ccgcgtgacg atcaacgtcc cgtgggcccc gagcgccggc gcgaccgcca gccggccggt
     1501 gcccgaggac agtagcgtgt gctcctcgct ggcgaggtgg cgctggaggc acccgcgcac
     1561 cgtgccgtcg ccgcgcgcgt cgatcaacag cccgcgcagc ggtcccgagc tgcgaaactc
     1621 gacccgcagg cgctccgact cgtgcttggt gagggtcgtg agcaggcagg ccgccgtcag
     1681 cgcacgaccg agggcgacga cctcgatccc tcgcaaggcg tgacgggcgc tggcctccgc
     1741 gacgaggccg gtcgtcaccg cggcgatgac ccgcagcgag aagtcggtct tgatggcgcg
     1801 ctggagctga tccaccgcgc cagcatagca gcagggcccg gggctgtctc gcgtccccgg
     1861 gacatgtcct tcagggcagg tcgaccagct tgaccttgac gctcgccgcc tgcccgtcgc
     1921 ggaccagctt gacggtgacc tcgtcgccga cctcgaagcg gtcgaaggcg ttgtagaggt
     1981 cgtcgaagtc ggcgaccacg tgttcgtcga tcccgacgat gatgtccccc agcgagatct
     2041 ccccgcgacg gtcctgtacc agcccgcgga tgcccgccag ctgcgccggc gagcccggcg
     2101 acacccgcgt caccaccacc cccttgatgc cggcgcgcgc cgcgtagtgg tccggcaacg
     2161 gttcgatgcc gatgccgggg tgctgcacgc gcccggtcgc gatcacctgc gggaccaccc
     2221 gccgcaccgc gctgaccggc accgcgaagc cgatgcccgc cgagctcccg gtcttgctga
     2281 agatcatcgt gttcatgccg atcaggtacc cgcccgagtc gagcagcggt ccgcccgagt
     2341 tgccggggtt gatcgacgcg tcggtctgga tcatgtcgcg aatggtcacg ccgccgtagc
     2401 cctgcacctc gcgcccgagc gccgagatta cgccggtcgt cagcgtgtgg tcgaggccga
     2461 aggggttgcc gatcgcgatc gccgtctgcc cgaccgcgag cttgacgtcc ggcggcagca
     2521 gcggcagcgg cgtcagcggc tcgttgcagc cgatcagctt caacaccgcg acgtccttgt
     2581 tcgggtcgcc cccgaggaac tccgcgtcgt aggtcttctg gctgtgcagc gtcaccgagt
     2641 agcggccctg cgcgcggccg ctgtcgacca cgtgatagtt cgtcaccacg tgcccctgcg
     2701 cgtcccacaa gaaaccagtg ccggtgcccg cggggatctc gagcgcccgc atcgagaggt
     2761 cgcgcaccat ccgcttctgg gtcacgaaca ccgtcgccgg cgcggccgcc cggaacacgt
     2821 cgatcacgtt ctgctcggcc tcgagccgcg cccccggaga cggcgggcgg gtcgtcggcg
     2881 tcgtcggcgg ggtcgccggg gccacgaccg tcggcaccgg caccgcctcg ggcacggccg
     2941 cgtgggcgat ctcgggggcc cgcggcaccg gcggcgccac ggtcgaacac tcggccgcgc
     3001 ccggtaacag caacaaacag cccgcgagag ccagcacgac cgacggtctg aatcgacgat
     3061 ggtgtcccac gcgcctcttc ttgcgtcgtc ccctgccgcc tcgcaagcgc cccgcggctc
     3121 cgcgcgccgc cggccggccg gggccatgac ctgcttttat gtccagccat ttgcggccgg
     3181 atcgcacgcg cgcggctgcg cgaggcggcc gatgggcgct ggcgtgttat aagttgccgg
     3241 ccatgaacgg tgctgccccc gcgccctccc tcggaccctg tccccactgc ggacagccgc
     3301 acgccgaaga cgtgctcgtc tgcccccagg agagcaaggt catgccgctc gagggccgca
     3361 tgctcgacaa caagttccgc ttcaccaagc tgctcggcgc gggcgggatg ggcgcggtgt
     3421 ggcgggcgca gaacatccgc gtgcgcaagg cggtcgcgat caagctgatg cacccggagt
     3481 tcgcgggcaa cccgggcatc ctcgaccgct tccgcaacga ggcgaccgcc gccggccaga
     3541 tcggcagcca gcacatctgc gacatctacg acttcggcgc gagcgtgctc ggcccgtaca
     3601 tcgtcctgga gatgctcaac ggcaagtcgt tcggcgagct gatccagcaa tacgggcgcg
     3661 tcgaccccgg cctcgccgcg atcatcatgc gtcaggcgct gaccggcctc gaggccgcgc
     3721 acatggtcgg catcgtccac cgcgacctca agccggagaa catcttcctg cacgagccga
     3781 gccccggccg gctgctcgtc aagctgatgg actttggcat ctcgaagttc tcgcagggcg
     3841 gcaacgacgg caagaccggc gtcggcgtgc tgatgggcac ccccgaatac atgtcgcccg
     3901 agcaggccga gggcgccgcc ggcgtcgacc agcgcaccga catctgggcg atgggcgcga
     3961 tcttgtacaa ggcgctcacc ggcgtcgagg ccttcggcgg cgccacgatg gccgccacgc
     4021 tcgtcgccct gtcgaccaag gagcccaagc cgatcgagtc gttcgccccg cacgtgcccg
     4081 cggagatgat cgccgtcgtc cgcaagtgcc tcgccaagga ccccgcggac cgctaccaga
     4141 gcgcccgcga gctgtccgac gcgctcgccc ccttcgagca gctgaccgga tcgatcccga
     4201 tgtcggcccc gcagagcagc gtcaccggcc tcgctaccac cgccccgcag ggcaccagcg
     4261 cccccctgca ggcccccacc ggcaccctca ccgcctcgag ctccggtccg gcgacgttca
     4321 tcaccccgca cggcccgccg agccagttcg gcggcccgtc cggcggcttc gcgccgaccc
     4381 acagccccgc cggcaacccc tccaccttcg tcggcggcac cctcggcgcc gccggccaca
     4441 ccgacgacag ctggtcgatc ggcgcccacg ccccggagac ccgcccgccg acgatctccg
     4501 gccgcgaagg cggcagcaag atcccgctcg tcatcctcgg cgtgctgctc ctggtcgcga
     4561 tcggcggcac cggctacttc ctgacgagcg gctcgcccaa acccggcaac atcgacggcc
     4621 cgcagatcga gcccgccacc accgcaggcg ccgtcgctgc gaccaccgcc ggcacgcccg
     4681 caggcaccac cgcaggcacg cccaccaccg caggtaccac gcccgaccct accgccggcg
     4741 cgaccgcggg caccaccgcc ggcacgccca ccggcacgac cgcgggaacc accgccggta
     4801 cgaccgccgg caccaccgcc ggcaccgacc cgacccccgg taccaccgcc ggcacaaccg
     4861 ccggtgccac gcccgacccg accgccggcg acgacgacga cgacaacaag ccgaccaagc
     4921 tgccgccgcc caagcccgac ctctcgctcg tctcgcagga gggcgccctg ttcacgccga
     4981 aggctccgtc cgcgagcgtg gcgaccgtcg acgaggccaa ggcggcatgc tcggcgctca
     5041 agtccaagaa gttcgccaag ctcacgcagt ggcggctcgc ctcctccggc gaggtgctca
     5101 agttcaaggg ccacaccgac gtgcaaaagc tcgcctactg ggtcacggac cccggcagcg
     5161 cgggtcgggc caagcgggtc gcgctggtga ccggcagcgt caacgagggc gaccccgcga
     5221 gcttcaagtc gacgcgcccc ttctgcgtaa ccaagcgctg atccgtcgcg gccgggcctg
     5281 gtggcctccg gcctccacgc gcgcgcgacg tgttagcgtc cccgcatgac ctacgacatg
     5341 cgcgacctct ccttcctggc ttgcacgctc ggcgcggcgc tggcgaccgg ctgcggcgac
     5401 aacggtggtg gtacctccac cgagaccggc acctcgacat ccagcgccga caccggcagc
     5461 accgacgcca cccagggccc gaccgacccc ggcacgaccg cggacgtccc gaccaccgac
     5521 ccgacgacca cggactcgac gacgagcctg accaccgacc cgacctcgac gaccgaggtc
     5581 acctcgacga ccgaccccgg cacgaccacc gacgacacca cgaccggcat gcccgcgtgc
     5641 ccctacacgc ccgtcgacgg tacgcctggt gtcggcctgg agccggtcgc cagcggcttc
     5701 gaccgcccgg tcctgaccgt ttcgcacccc aagcaaccgg accgtctgtt catcgtcgag
     5761 cagggcggcc acatcaagat cctcgagccc ggcatgacga tggcgccgcc ggactcggac
     5821 gactttctct acgtcaaggt caagaacgag aacgccaacg agatcggcgc cgagcagggc
     5881 ctgctcggct tcgcgttcca ccccaa
//
LOCUS       DASWNJ010000290         3941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_111482, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000290 DASWNJ010000000
VERSION     DASWNJ010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111482"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1349
                     /locus_tag="VGB85_05255"
     CDS             <1..1349
                     /locus_tag="VGB85_05255"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0133460.1"
                     /translation="KYAAAVDLARAQQEPAAAHTPTLAEARLALGTTLDHDGQLPAAR
                     EALQDAARLALAVGDDERALAAAIGLAGLTGIRMSRYDEGEGWLQVATGLAARVPEGD
                     EHIHLARVSCSYYNDRGMYEPALPHCERGLQLAEALHGPGGLETARALLGLANNHLKA
                     RRPDPARTLLARAWDIERGLLGEHHPALLGLANSRAAAEFYAGELDAAVARWHEGLAI
                     AEASVGRDHLQAGLIHINLAVVAIDQRAWDQAARELAAIERIYLPRADKLSAPLVFIH
                     FVRGRLAFARGDLASARENFEQQLHFARLSRSPEHPDVLKATLDLGDVLTVTGDLAGS
                     ERHHQDAHALADKLTDPESRALALRGLCRARVLLGRPAEAVADCEQALTLAAGSERNE
                     LLRSELRAWLARALLDSGRDPTRAGDMASAARDELRGLAEPGLEVLAMLDRPASPR"
     gene            complement(1370..2239)
                     /locus_tag="VGB85_05260"
     CDS             complement(1370..2239)
                     /locus_tag="VGB85_05260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0133461.1"
                     /translation="MMRDMHGKTALVTGSNTGIGKQTAIALARMGARVTITSRDREKG
                     RVALAEVRRASGNDAVTCMHLDLASLASVRSFADELLATHPRLDVLVNNAGQTLSARR
                     ETQDGFEETFGVNHLGHFALTGLLLPRLRASAPARVVVVSSVGHQLARGGLDFDDLHA
                     RRGYSGMGAYCRSKLANLLFVLELAGRLQGTGVTVNAVHPGVVKTDMGNSEDLQGWYR
                     LLVRLSHPFARTAEQGARTSVHVAAAAELDGVTGEYFADSELALRGPRALDRDAARRL
                     WQISEQLTGVQYE"
     gene            complement(2273..2674)
                     /locus_tag="VGB85_05265"
     CDS             complement(2273..2674)
                     /locus_tag="VGB85_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133462.1"
                     /translation="MQRRLVMSLLAALSSGACTDRTIAGAGDEDPEVDPGEQPSAAGA
                     LYSPCTDSSECPELLCVFPSGEAGFCSAPCEALDDPGRCEPTPGDQPATCLDIGLPVT
                     ACALDCEDAPCPVGMRCERVLSGDDERSICF"
     gene            complement(2759..3637)
                     /locus_tag="VGB85_05270"
     CDS             complement(2759..3637)
                     /locus_tag="VGB85_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133463.1"
                     /translation="MRRRAGAWSFAALGFAVGLACDLRPNSPRSTPECPACECTCECP
                     AVATPMTTPVDPKAGPTEADIGELMGSAVRKMNFEDGAGCLADLDRIDALDPKQGARQ
                     AMIRGQCEMLAGKCQQGKQRVMRWYQDETDMQPGRALVTAEALASMRCREGDSDERDR
                     LLRALYDLSDGAYMNKRAPEFCEKSLAEARRLIPIVKTRGPDDTTIAGGAQALFHTAA
                     SCFARAGDCDAAWSTYRELFPNASAPATPEVAAMMQQVPQMQQIIRDAYDSSILFCGA
                     QKRGPAQAEPPPPPPR"
     gene            complement(3646..>3941)
                     /locus_tag="VGB85_05275"
     CDS             complement(3646..>3941)
                     /locus_tag="VGB85_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133464.1"
                     /translation="NLKRMSVGECDRAYQQVRRNRDAVQPRDEEDHQVLQARRDLYAI
                     APGCFGRAGDCEVARRVFNDNFPLDRLKDVKDPKVRAEAITSTFEAIVPKCKR"
BASE COUNT          517 a         1522 c         1348 g          554 t
ORIGIN      
        1 gcaagtacgc cgccgccgtc gacctcgcgc gggcccagca agagcccgcc gccgcccaca
       61 cgccgaccct cgccgaggca agactcgcgc tcggcaccac gctcgatcac gacggccagc
      121 tcccggccgc ccgcgaggcg ctgcaggacg ccgcgcgcct ggccctcgcg gtcggtgacg
      181 acgagcgggc cctcgccgcc gcgatcggcc tcgccggcct gaccggcatc cgcatgtcgc
      241 gctacgacga gggcgagggc tggttgcagg tcgccaccgg cctggccgcg cgtgtgcccg
      301 agggcgacga gcacatccac ctcgcgcgcg tcagttgcag ctactacaac gaccgcggca
      361 tgtacgagcc ggccctgccg cactgcgagc gcggcctgca gctggccgag gcgctgcacg
      421 gacccggcgg cctcgagacc gcgcgcgcgc tgctcggcct cgccaacaac cacctcaagg
      481 cgcgccgccc cgaccccgcg cggacgctgc tggcgcgcgc ctgggacatc gagcgcggcc
      541 tgctcggcga gcaccacccg gcgctgctcg gcctggcgaa cagccgcgcc gccgccgagt
      601 tctacgccgg tgagctcgac gccgccgtcg cgcgctggca cgagggcctg gcgatcgccg
      661 aggccagcgt cggccgcgat cacctgcagg ccggcctgat ccacatcaac ctcgccgtcg
      721 tcgcgatcga ccagcgcgcc tgggaccagg ccgcccgcga gctcgcggcg atcgagcgca
      781 tctacctccc ccgcgccgac aagctcagcg cgccgctggt cttcatccac ttcgtgcgcg
      841 gccgcctcgc cttcgcccgc ggcgacctcg cgagcgcccg cgagaacttc gagcaacagc
      901 tgcacttcgc ccgcctcagc cgctcgcccg agcaccccga cgtgctcaag gcgaccctcg
      961 acctcggcga cgtgctcacc gtcaccggcg acctcgcggg ttcggagcgc caccaccaag
     1021 acgcccacgc gctcgccgac aagctgaccg accccgagtc ccgcgcgctc gccctgcgcg
     1081 gcctgtgtcg cgcgcgcgtg ctgctcggtc gtcccgcgga ggccgtcgcc gactgcgagc
     1141 aggcgctgac gctcgccgcc ggcagcgagc gcaacgagct gttacgcagc gagctgcgcg
     1201 cctggctcgc gcgcgccctg ctcgactcgg gtcgagatcc cacccgcgcc ggagacatgg
     1261 ccagcgctgc gcgcgacgag ctgcgcggcc tcgccgagcc cggcctcgag gtgctggcga
     1321 tgctcgaccg cccggcctca ccgcgctgag gttcacgagc accgcgcagt tactcgtact
     1381 gcacgccggt cagctgctcg ctgatctgcc acagacgtcg cgcggcgtcg cggtcgagcg
     1441 cccgggggcc gcgaagcgcg agctcgctgt ccgcgaagta ttcgccggtc accccgtcca
     1501 gctccgccgc cgcggcgacg tgcaccgagg tgcgcgcgcc ctgctcggcc gtccgcgcga
     1561 acgggtggct gagccgcacc agcaggcgat accagccctg caagtcctcg ctgttgccca
     1621 tgtccgtctt gacgacgccc gggtgcacgg cgttgacggt gacgccggtg ccctgcagac
     1681 gcccggccag ctcgagcacg aacaacaggt tggcgagctt ggaccggcaa tacgcgccca
     1741 tcccgctgta gccgcgacgc gcgtggaggt cgtcgaagtc gaggccgccg cgcgccagct
     1801 ggtggccgac cgaggatacg acgacgacgc gggccggggc gctggctcgc aggcgcggga
     1861 gcagcaggcc ggtcagcgcg aagtgtccga ggtgattgac gccgaaggtc tcctcgaagc
     1921 cgtcctgcgt ctcacgccgc gcgctgagcg tctgcccggc gttgttgacg aggacatcga
     1981 ggcgcgggtg ggtggcgagc agctcgtcgg cgaagctgcg tacgctcgcc agcgacgcga
     2041 ggtcgaggtg catgcaggtg acggcgtcgt tgcccgacgc gcggcgaacc tccgcgagcg
     2101 cgacccgacc cttctcgcgg tcccgcgacg tgatcgtcac cctcgccccc atcctggcca
     2161 gcgcgatcgc cgtctgcttg ccgatcccgg tgttgctgcc ggtcaccagc gcggtctttc
     2221 catgcatgtc gcgcatcatc gccgtctcct accagcttcc ccgcggcccc ggctagaagc
     2281 agatcgaccg ctcgtcgtcg ccggacagca cccgctcgca gcgcatgccg accgggcacg
     2341 gcgcgtcctc gcagtcgagc gcgcaggcgg tgaccggcag gccgatgtcg aggcaggtcg
     2401 cgggctgatc cccgggggtc ggctcgcagc gtcccgggtc gtcgagggcc tcgcacgggg
     2461 ccgagcagaa gccggcctcg ccgctgggga atacgcacag cagctccggg cactcgctcg
     2521 agtcggtgca cggcgagtac aacgcccccg ccgcgctcgg ctgctcaccg gggtcgacct
     2581 ccggatcctc gtcgccagcc ccggcgatcg ttcgatcggt gcacgccccc gacgagagcg
     2641 ccgcgagcag ggacatcacg agccgtcttt gcatgctccg tcccaacgga cctccgcgtt
     2701 cgccggtctg cgatcgcagc tcgaattgtt tttcgtgcac acgcaccacc attcgcgctc
     2761 acctcggcgg cggaggcggg ggctcggcct gcgccgggcc ccgcttctgg gccccgcaga
     2821 acaggatgct ggagtcatac gcgtcgcgga tgatctgctg catttgcggc acttgctgca
     2881 tcatcgcggc gacctccggc gtcgccgggg cgctcgcgtt ggggaacagc tcgcggtagg
     2941 tcgaccacgc cgcgtcgcag tcgcccgcgc gcgcgaagca cgaggccgcg gtgtggaaca
     3001 gcgcctgcgc cccgccggcg atcgtggtgt cgtccgggcc ccgcgtcttg acgatcggga
     3061 tcaggcgccg cgcctccgcg agcgacttct cgcaaaactc cggcgcgcgc ttgttcatgt
     3121 aagcgccgtc cgagaggtcg tacagcgccc gcagcaggcg gtcgcgctcg tccgagtcgc
     3181 cctcgcggca gcgcatcgag gcgagcgcct ccgcggtgac cagcgcgcgc cctggttgca
     3241 tgtcggtctc gtcctgatac cagcgcatca cccgttgttt gccctgctgg cacttgccgg
     3301 ccagcatctc gcactgcccg cggatcatcg cctgccgcgc gccctgcttc gggtcgagcg
     3361 cgtcgatgcg gtcgaggtcc gccaagcagc cggcgccatc ctcgaaattc atcttgcgca
     3421 ccgccgagcc catcagctcg ccgatgtccg cctcggtcgg cccggccttc ggatcgaccg
     3481 gtgtcgtcat cggcgtcgcc acggccgggc actcgcacgt gcactcgcag gccgggcact
     3541 cgggcgtgct ccgggggctg ttgggccgca ggtcgcaggc caggccgacc gcgaacccga
     3601 gggcggcgaa gctccaggca cccgctcgtc tgcgcatggt cgacctcatc gcttgcactt
     3661 cgggacgatc gcctcgaagg tcgaggtgat cgcctcggcc cggacctttg gatccttgac
     3721 gtccttgagc cggtcgagcg ggaagttgtc attgaagacc cggcgcgcga cctcgcagtc
     3781 gcccgcgcga ccgaagcagc cgggggcgat cgcgtacagg tcgcgccggg cctggagcac
     3841 ctggtggtcc tcctcgtcgc gcggctgcac ggcgtcgcgg ttgcggcgga cctgctggta
     3901 ggccctgtcg cactcgccca cgctcatgcg cttgaggttg g
//
LOCUS       DASWNJ010000291         3605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_288244, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000291 DASWNJ010000000
VERSION     DASWNJ010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288244"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1894)
                     /locus_tag="VGB85_05280"
     CDS             complement(<1..1894)
                     /locus_tag="VGB85_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY0133465.1"
                     /translation="MHRLPTRLASILWTLGLMAPGGCAGGEASETGATAADSSGAGPT
                     SFPGSSEPTGDPTGDPTGDLGTTGDPTTTTTTSGSDTTGDDPTTGGDEVLCPTAFQFD
                     PPPGATAPKIAGEWQGFDLATAAVMTGPDAEGRWQATIPLAPGLHGYKVVYEQGGQPQ
                     WVLDAGQGRRKYVGGVENSAVLVPDCRDPALVVATSAASRPKQDAGAYAATVRYVDGL
                     AGAGPDVANYEAVLRDSSGKQRALTADELTIDAATGDVTIAVAGLGDGKHRVTLTARS
                     ADGRASAPLRLIFWIEAQAFTWDGALVYMVMTDRFVDGDPGNNNGATPGADPRGDWKG
                     GDLEGLRAAIADGTLDELGVRAIWLTPFQTNPKQAFVASDGVHQVTGYHGYWPIKARE
                     VDPRLGGAEALHAMVREAHAHGIRILQDYVVNHVHEQHEYFTAHPEWFRTGCVCGTDN
                     CDWTGHALDCLFSQYLPDINHSVPEANAAFVADAVYWLDEFDLDGLRVDAVKHVEEVA
                     TRNLAAAVRESFEPAGTKYFLMGETAMGWSDGPDPANDENYGTIARYIGPFGLDGQFD
                     FVLYHGVSYRTFAYHDKGMLHADYWTTHGLEKWPEGAIMTPYIGSHDTPRFVSLADYR
                     GQDAQHD"
     gene            2038..>3605
                     /locus_tag="VGB85_05285"
     CDS             2038..>3605
                     /locus_tag="VGB85_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133466.1"
                     /translation="MLPRPRRLLASRFIVSRFIVSGAVLLLAHIGTAAAAPAPQRTRF
                     ELPSSNGHGAILLDLKQRRLVQFREHLFAAEEPVLDDQGKEVWDGEQFATVHTRDLLF
                     DTYFGVRADGQQMWLTDVPVDEDASGYLPWAEGEQGGTGIITMVQTVGALRATTYAFA
                     PQALAAGSYIMLLKLENTGDVAFKGVGAFSLHNYHLGYGRALRPWDVPKDIAANGEII
                     EYDNSGGPIRFRERGFAGVIVTRGLGAIAHSGVGPDVDLYNLVKGGLPKDFPDNQPPV
                     NAIDDAVSGFQWTLGDLAPGATQWAGVVVLHDPDPLPDKQAQEVVDDFVGGKNAKALL
                     DAELAAWAELQADVVVPAGASPQETAQIRQGAVNLHMGQVQEDSYYLREWLSKDGEPR
                     RTRFKGVDDQPVTLPAKIKHRGFGAVLASLPPGEWTYAWIRDGAYATAALATLGLEDH
                     TKRALEFYLQAEAGRFQGWDELKPYGMPPYQISLVRYHGFGVEETDFNAFGPNLEFDG
                     FGLFLWALRVHEQR"
BASE COUNT          488 a         1360 c         1209 g          548 t
ORIGIN      
        1 tgtcgtgctg cgcgtcctga ccgcggtagt ccgcgaggct gacgaagcgc ggcgtgtcgt
       61 ggctgccgat gtacggcgtc atgatcgccc cctccggcca cttctcgagc ccgtgcgtgg
      121 tccagtagtc ggcgtgcagc atgcccttgt cgtggtacgc gaacgtccgg tagctgaccc
      181 cgtggtacag cacgaagtcg aattgtccgt cgagcccgaa cgggccgatg tagcgcgcga
      241 tcgtgccgta gttctcgtcg ttcgccgggt cgggcccgtc gctccagccc atcgccgtct
      301 cgcccatcag gaagtacttg gtgcccgccg gctcgaagct ctcgcgcacc gccgcggcga
      361 ggttgcgcgt cgcgacctcc tcgacgtgct tgaccgcgtc gacgcgcagg ccgtcgaggt
      421 cgaactcgtc gagccagtac accgcgtcgg cgacgaacgc cgcgttggcc tccggcaccg
      481 agtggttgat gtcgggcagg tactgcgaga acaggcagtc gagcgcgtgc ccggtccagt
      541 cgcagttgtc cgtgccacac acgcagccgg tgcggaacca ctccgggtgc gccgtgaagt
      601 actcgtgctg ctcgtggaca tggttgacca cgtagtcctg aaggatccgg atcccgtgcg
      661 cgtgcgcctc gcggaccatc gcgtgcagcg cctcggcgcc gccgagccgc gggtcgacct
      721 cacgcgcctt gatcggccag tagccgtggt agccggtcac ctgatgcacg ccgtcgctgg
      781 cgacgaacgc ctgcttcggg ttggtctgga acggcgtcag ccagatcgcc cgcacgccga
      841 gctcgtcgag cgtgccgtcg gcgatcgccg cgcgcaggcc ctcgagatcg ccgcccttcc
      901 agtcgccgcg cgggtcggcc ccgggcgtcg cgccgttgtt gttgcccggg tcgccgtcga
      961 cgaagcggtc cgtcatcacc atgtagacca gcgcgccgtc ccacgtgaac gcctgcgcct
     1021 cgatccagaa gatcaggcgc agcggcgccg aggcccggcc gtccgccgag cgggcggtca
     1081 gcgtgacccg gtgcttgccg tcccccaggc ccgcgaccgc gatcgtgaca tcaccggtcg
     1141 ccgcgtcgat cgtcagctcg tccgccgtca gcgcgcgctg cttgcccgag ctgtcccgca
     1201 ggacagcctc gtaattggcg acgtccggcc cggcccccgc gaggccgtcg acataccgca
     1261 ccgtcgccgc gtacgcgccc gcgtcctgct tgggccggct cgccgcgctg gtcgcgacca
     1321 ccagcgcggg gtcgcggcag tcgggcacca gcaccgccga attctcgacc ccgccgacgt
     1381 acttgcgccg cccctggccg gcgtcgagca cccactgcgg ctggccgccc tgctcgtaga
     1441 ccaccttgta gccgtgcagg ccgggcgcca gcgggatcgt cgcctgccac ctgccctcgg
     1501 cgtcggggcc cgtcatcacc gcggcggtcg cgaggtcgaa gccctgccac tcgcccgcga
     1561 tcttcggcgc cgtcgcgccg ggcggggggt cgaattgaaa cgcggtcgga cacagcacct
     1621 cgtcgccgcc ggtcgtcgga tcgtctccgg tcgtgtcgct gccgctcgtc gtcgtcgtgg
     1681 tcgtcggatc gccggtcgtg cccagatcgc cggtcgggtc gccggtcggg tcgccggtcg
     1741 gctcgctgct cccggggaaa ctcgtcgggc cggcgccgct gctgtccgcc gccgtcgcgc
     1801 cggtctccga ggcttcaccc ccggcgcagc cgccgggcgc catcaggccc aaagtccaca
     1861 ggatcgaggc gagacgcgtt ggcaggcgat gcatgtcggc acggcaccat agccggtcac
     1921 gcattccctg caaagccgcc gagcctgcgg acccccgttt cactcgtttc actcggtgca
     1981 gacggcgcca gcgctcccgg ggccggccgc ggtccggtcc tgctacgatg gcgcccgatg
     2041 ctcccccgtc ctcgccgcct cctcgcgtct cgcttcatcg tgtctcgctt catcgtctcg
     2101 ggcgccgtcc tgctgctcgc ccacatcggg accgcggcgg ccgcaccggc gccccagcgc
     2161 acgcgcttcg agctgccctc gtcgaacggc cacggcgcga tcctcctcga tctcaagcag
     2221 cgccggctcg tgcagttccg cgagcacttg ttcgcggcgg aggagccggt gctcgacgac
     2281 cagggcaagg aggtctggga cggcgagcag ttcgcgaccg tacacacccg cgacctgctg
     2341 ttcgacacct acttcggcgt gcgggccgac ggccagcaga tgtggctgac cgacgtcccc
     2401 gtcgacgagg acgccagcgg ctatttgccg tgggccgagg gcgagcaggg cggtacgggc
     2461 atcatcacga tggtccagac cgtcggcgcc ctgcgtgcga cgacctacgc gttcgccccg
     2521 caggcgctcg cggccggcag ctacatcatg ctcctcaagc tcgagaacac cggcgacgtc
     2581 gccttcaagg gcgtcggcgc cttctcgctg cacaactacc acctcggcta cggccgcgcg
     2641 ctgcgcccgt gggacgtgcc caaggacatc gcggccaacg gcgagatcat cgagtacgac
     2701 aacagcggcg gcccgatccg cttccgcgag cgcggcttcg ccggcgtgat cgtcacgcgg
     2761 ggcctcggcg cgatcgccca ctccggtgtc ggccccgacg tcgacctcta caatctcgtc
     2821 aagggcggtc tgcccaagga cttcccggac aaccagccgc cggtcaacgc catcgacgac
     2881 gccgtcagcg gcttccagtg gaccctcggt gatctcgccc cgggggcgac ccagtgggcc
     2941 ggcgtcgtcg tcctgcacga ccccgatccg ctgcccgaca agcaggcgca ggaggtggtc
     3001 gacgacttcg tcggcggcaa aaatgccaag gccctgctcg acgcggagct cgccgcgtgg
     3061 gccgagctcc aggccgacgt cgtcgtgccc gccggcgcca gcccgcagga gaccgcgcag
     3121 atccggcaag gcgccgtcaa cttgcacatg ggacaggtcc aggaggacag ctactacctg
     3181 cgcgagtggc tgagcaagga cggcgagccg cgacgcaccc gcttcaaggg cgtcgacgac
     3241 cagccggtca cgctgcccgc gaagatcaag caccgcggct tcggggcggt gctcgccagc
     3301 ctcccgcccg gcgagtggac ctacgcctgg atccgcgacg gcgcctacgc gaccgccgcg
     3361 ctggcgaccc tcggcctcga ggaccacacc aagcgcgcgc tcgagttcta cctccaggcc
     3421 gaggccggcc gcttccaggg ctgggacgag ctcaagccct acggcatgcc gccgtaccag
     3481 atcagcctgg tgcgctacca cggcttcggc gtcgaggaga ccgacttcaa cgccttcggc
     3541 cccaacctcg agttcgacgg ctttggcctg ttcctgtggg cgctgcgcgt gcacgagcag
     3601 cgcag
//
LOCUS       DASWNJ010000292         1751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_429702, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000292 DASWNJ010000000
VERSION     DASWNJ010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429702"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1717)
                     /locus_tag="VGB85_05290"
     CDS             complement(<1..1717)
                     /locus_tag="VGB85_05290"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEY0133467.1"
                     /translation="MTPLGDTFTAELAQFGPTEFDLQATLVLAEDTGDKSPTDACQAI
                     VNNVAGKIVLVDRGDCTFETKVTFAQAAGAVGVVIADNVDAATPPALGNDGTLADPTI
                     PAQGVTKATGALLKAALENGAQTAHMVGATSVERDGTIDNMIVAHEWGHYIHHRLVEC
                     GNNACAAQSEGWGDFVAVHMMLREGDPLGAVYAADTYASFDLTGYFGIRRVPYSVDFT
                     KNALTYRHIADGEMLPDDHPIESGGISNSEVHNAGEVWATMMWEAYIALHEAHQGQES
                     FAEIRRLFADYVVAGMILAPPSPTFTEQRDALLMAAAARSNADFTTIAEAFARRGAGS
                     CAVSPPKNSVDFKGVVEDFQLRANGVLLTATIGEGDASCDGDGVIDIGEDGRIDLEFY
                     NGGAALLPPGSKIEVIDPDPALVFPNGSVVTMPELAPLAKTTASIEIAVAGLTDYRPV
                     DVKLRVTTIDSCNEVSERVLPIFIHADLAANVSAVDDVEAEPSVWKLTGGGESVWRRA
                     ASEQDGFYWHADDVGRQSDTRLNSPALIVAEDAPLRVAFDHAFSFETSDGINWDGAVI
                     ELTRDA"
BASE COUNT          229 a          596 c          634 g          292 t
ORIGIN      
        1 cggcgtcgcg cgtcagctcg atcaccgcgc cgtcccagtt gatcccgtcc gaggtctcga
       61 agctaaacgc gtggtcgaac gcgacccgca gcggcgcgtc ctcggcgacg atcagggccg
      121 gtgaattcag gcgcgtgtcg ctctgccggc cgacgtcgtc ggcgtgccag taaaagccgt
      181 cctgctcgct cgcggcgcgg cgccacacgg actcgccgcc gccggtcaac ttccacacgc
      241 tcggctcggc ctcgacgtcg tcgacggcgg agacgttggc cgcgaggtcg gcgtggatga
      301 agatcggcag cacccgctcg ctgacctcgt tgcagctgtc gatcgtggtc acgcgcagct
      361 tgacgtcgac cgggcggtag tcggtgagcc cggcgaccgc gatctcgatg ctcgcggtgg
      421 tcttggccag cggcgccagc tcgggcatcg tcaccaccga gccgttgggg aacaccagcg
      481 cggggtcggg gtcgatcacc tcgatcttcg agccgggggg cagcagcgcg gcgccgccgt
      541 tgtagaactc gaggtcgatg cgcccgtcct cgccgatgtc gatcacgccg tcgccgtcgc
      601 acgaggcgtc gccctcgccg atcgtggcgg tcagcagcac gccgttggcg cgcagctgga
      661 agtcctcgac cacgcccttg aagtcgaccg agttcttggg cggtgagacg gcgcagctgc
      721 cggcgccgcg gcgcgcgaag gcctcggcga tcgtcgtgaa gtcggcgttg ctgcgcgccg
      781 cggcggccat cagcagcgcg tcgcgctgct cggtgaaggt cggggacggc ggggcgagga
      841 tcatgccggc gaccacgtag tcggcgaaca gccgccggat ctccgcgaag ctctcctggc
      901 cctggtgggc ctcgtgcagc gcgatgtagg cctcccacat catcgtcgcc cacacctcgc
      961 cggcgttgtg cacctcgctg ttgctgatcc cgccgctctc gatcgggtgg tcgtcgggca
     1021 gcatctcgcc gtcggcgatg tggcggtagg tcagcgcgtt cttggtgaag tcgaccgagt
     1081 aggggacgcg acggatcccg aagtatccgg tcaggtcgaa gctggcgtag gtgtcggccg
     1141 cgtacacggc gccgagcggg tcgccctcgc gcagcatcat gtggaccgcc acgaagtcgc
     1201 cccagccctc gctctgcgcg gcgcaggcgt tgttgccaca ctcgacgagg cgatggtgga
     1261 tgtagtgacc ccactcgtgg gcgacgatca tgttgtcgat cgtgccgtcg cgctcgacgc
     1321 tggtcgcccc gaccatgtgg gctgtctggg cgccgttctc gagggcggcc ttgaggaggg
     1381 cgccggtcgc cttggtgacg ccctgcgcgg ggatcgtcgg gtccgccagc gtgccgtcgt
     1441 tgccgagcgc cggcggcgtc gcggcgtcga cgttgtcggc gatcacgacc ccgaccgcgc
     1501 cggccgcctg ggcgaacgtc accttggtct cgaaggtgca atctccgcgg tcgacgagca
     1561 cgatcttgcc ggcgacgtta ttgacgatcg cctgacaggc gtcggtcggg ctcttgtcgc
     1621 cggtgtcctc ggcgagcacc agcgtcgcct ggaggtcgaa ctcggtcggc ccgaactgcg
     1681 cgagctcggc ggtgaaggtg tcaccgagcg gggtcaccgt cagatccgag gcgatcgtgc
     1741 gcccggtcca c
//
LOCUS       DASWNJ010000293         2326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_392925, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000293 DASWNJ010000000
VERSION     DASWNJ010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392925"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1559)
                     /locus_tag="VGB85_05295"
     CDS             complement(60..1559)
                     /locus_tag="VGB85_05295"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY0133468.1"
                     /translation="MELLDNLSLGFATALTPWNLLYAFVGCVLGTAVGVLPGLGPVAT
                     IAMLLPLTFGLPPVSSLIMLAGIYYGAQYGGSTTAILINLPGESSSVVTAIDGYQMAR
                     QGRAGQALATAGLGSFFAGSVATLLLAAAAPPLAEVALEFGPAEYFSLMVLGLVASVA
                     LASGSLLKAFAMIVLGLLLGLVGSDVNTGAQRYTFGLPEFADGLNFVALSMGVFGLGE
                     ILRNLEHEITRDVMVRRVTGLMLSRDDLRRIAGPVLRGTALGSVLGILPGGGAMLASF
                     AAYTVEKRISPNRAEFGRGAIEGVAAPEAANNAGAQTSFIPMLTLGIPSNPVMALMVG
                     ALIIQGIQPGPNVVTDEPELFWGLIASMWIGNLMLVLLNLPLIGMWVRMLTIPYHFLF
                     PGIIAFCCIGAFSVNNNLFDVYAMALFGVVGYALIKLDFEPAPLLLGFVLGPLLEENL
                     RRAMLISRGDATVLVTQPLSLALLVVAAALLAVVVLPGIRARRQEAFQE"
     gene            complement(1570..2100)
                     /locus_tag="VGB85_05300"
     CDS             complement(1570..2100)
                     /locus_tag="VGB85_05300"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEY0133469.1"
                     /translation="MIERPRDVIGGVVVLAIGLAFLLAGRGLETGSAARMGPGWFPTV
                     LSCLMMALGAALALLALRRPGEEAWLGRVPWRGVALVIGAVVLSGLALRRLGLAPVVL
                     AVVLATAWASRYATWRASVPLALGLAAFCTLLFVRGLGLPLPTLGPWLTPAYWSPPPV
                     APPAATPPAATPPAAP"
BASE COUNT          375 a          833 c          859 g          259 t
ORIGIN      
        1 cgggcggttg gtcgacgtgg gccagggagc cgtcgtcgcg cgttcggctc cccgcgccgc
       61 tactcctgga aggcctcctg gcgcctggcg cggatgccgg gcagcacgac gacggcgagc
      121 agcgccgccg cgaccaccag cagcgccagg ctcagcggct gggtgacgag gacggtggcg
      181 tcgccgcggc tgatcagcat cgcccggcgc aggttctcct ccagcagcgg cccgagcacg
      241 aagcccagca gcagcggcgc cggctcgaag tcgagcttga tcagcgcgta gccgaccacc
      301 ccgaacagcg ccatcgcgta gacgtcgaac aggttgttgt tcacgctgaa cgcgccgatg
      361 cagcagaacg cgatgatgcc cgggaacagg aagtggtagg ggatggtcag catcctgacc
      421 cacatcccga tcagcggcag gttcagcagc accagcatca ggttgccgat ccacatcgag
      481 gcgatcaggc cccagaacag ctccggctcg tcggtgacca cgttcggccc gggctggatg
      541 ccctggatga tcagcgcgcc gaccatcagc gccatcaccg ggttcgacgg gatgcccagc
      601 gtcagcatcg ggatgaacga ggtctgcgcc ccggcgttgt tggccgcctc cggcgccgcc
      661 accccctcga tggcgccccg gccgaactcg gcccggttgg gcgagatcct cttctccacc
      721 gtgtaggcgg cgaacgaggc cagcatcgcc ccgccgccgg gcaggatgcc cagcacggag
      781 ccgagcgcgg tgccgcgcag caccgggccg gcgatgcgcc gcaggtcgtc gcgcgagagc
      841 atcagcccgg tgacgcgcct gaccatcacg tcgcgcgtga tctcgtgctc gaggttgcgc
      901 aggatctcgc cgagcccgaa cacgcccatg ctgagcgcca cgaagttgag cccgtcggcg
      961 aactcgggca ggccgaaggt gtagcgctgc gcgccggtgt tcacgtccga gccgaccagc
     1021 ccgagcagca ggcccagcac gatcatcgcg aacgccttga gcagcgagcc gctggccagc
     1081 gccaccgagg ccaccagccc gagcaccatg agcgagaagt actcggccgg gccgaactcg
     1141 agcgcgacct cggcgagcgg gggcgcggcg gcggcgagca gcagggtcgc caccgagccg
     1201 gcgaagaacg agcccaggcc ggcggtggcg agcgcctgcc cggcgcggcc ctgccgcgcc
     1261 atctggtagc cgtcgatggc ggtcaccacc gaggaggact cgccgggcag gttgatcagg
     1321 atggcggtgg tcgagccgcc gtactgggcg ccgtagtaga tgccggccag catgatgagc
     1381 gaggagaccg gcggcaggcc gaaggtgagc ggcagcagca tggcgatggt ggcgaccggg
     1441 ccgaggccgg gcagcacgcc gaccgcggtg ccgagcacgc agccgacgaa ggcgtagagc
     1501 aggttccagg gggtcagcgc cgtggcgaag ccgagggaga ggttgtcgag gagttccatc
     1561 gcggggcccc tatggcgcgg cgggcggcgt ggcggcgggc ggtgtggccg ccggcggcgc
     1621 gaccgggggc ggcgaccagt aggccggcgt gagccagggg ccgagcgtcg gcaggggcag
     1681 gccgaggccg cggacgaaca ggagcgtgca gaaggcggcg aggccgagcg cgagcggcac
     1741 cgaggcgcgc caggtggcgt agcggctggc ccaggcggtg gcgagcacga cggcgaggac
     1801 gaccggggcg aggccgaggc gcctcagggc caggccggag aggaccaccg cgccgatcac
     1861 cagggcgacg cccctccagg gcacccggcc gagccaggcc tcctcgccgg gcctgcgcag
     1921 ggcgagcagc gcgagggcgg cgccgagcgc catcatcagg cagctcagca cggtcgggaa
     1981 ccagcccggc cccatccggg ccgcgctgcc cgtctccagc ccgcgccccg cgaggaggaa
     2041 ggcgaggccg atcgccagga cgaccacccc gccgatgacg tccctcggcc tctcgatcac
     2101 tgcggccacc tcagctcccc cctgctggtt gttctcgtcg cccgaccacc cccaaagtcc
     2161 tcgtcgccag cgcggggttg ccgcgatctt ccgccccctc cggcgtcgcc agggcaccct
     2221 ccgcgacgag ggcagcgctc tccgcccccg cctcacgtcc gccaatgcga atccatggat
     2281 cagtcttgcc tacctcgaca cagatcgacg gtcgcatcac ggcttt
//
LOCUS       DASWNJ010000294         6083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_146711, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000294 DASWNJ010000000
VERSION     DASWNJ010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6083
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146711"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5365)
                     /locus_tag="VGB85_05305"
     CDS             complement(<1..5365)
                     /locus_tag="VGB85_05305"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY0133470.1"
                     /translation="MVEEDQTGAMCESDGASFAVDYEQFVDVEWSDAEDLQWDEGDGE
                     AELDGPALDFLAFGALIEIDDSSYERWLQSCLKGLVAPDLVVDGVPGGKTRQAIRAFQ
                     RQVGRLRPGARALTVDGVDGPATVAALELCTNSTHPPRATGKPATPAVVVPESMPAQP
                     GTQRGVFTLTTTDGAHHTVQAYGDRVSFRYKTRDRTGVDGKTLCDAFNVMAYDGARRD
                     LLRDEMLIARGFSASEIAILRENAFKESRGSFGAVNSWDDQIVSWGMAQFAGHAGSLA
                     GLLIFLKEHAPGAFARFFLAAGIDVEHGPYPYFDRKARCEISRTGAHVKIEADGVVYR
                     GDRGWAYLRTQPVLVGALMLAGNDVEVQVGQCLFWKEMFLDRAVATRIGGRTSHRVVR
                     VHGTGASRSETTLLAGADSEAAWQAWAARPENLIEGKNLARLLGRADRHVHRTREGDR
                     VVVTVEKVPGQPLRSFCSSTYGLGIMARLYNWMPAYVRTWSEEFLKELAASHSGVDVA
                     DPAAWDREPALETEFIEKVKARRRQVKKGSYDPPYGDTLNRARGSFTAGAAVELPAAP
                     DGGDEASDEEAAIIAADPERPQAGAIAELVLEAVVVTAGVDEPPAVRSSVSGVVAPGG
                     AGSAVVADTTEPVPEDRPDFDVTAQSPGEKRKRTRAWSELKGITLHQTGVHGFGARAW
                     PRVTAHMGVHSDGRVYLIHPLQAYLWSSDALNRDTVAIEVAGNFIGDPARPASFWKQG
                     GGPSTLTPAMIDGLRRAIRWIVAEVARNGGEITDVFAHRQAKRGKSNCPGAEIWLHAG
                     VWAQTELGLGNGGPGFTRSDGQPIPPAWDPRLLDQPLPFADPDAYVIDFEAMTAEEGA
                     EFPAEGEDPFAARPEVVASAVRIRSLPAGMRLAESGRDETVTRGRLDLSAPIDLDAAE
                     DVAITGQLDEALARQGLVVIDTITLEPDSGARARSLDAIDLDGAADDALEFDITIEED
                     ERAVVLLEQDGMFCWSLPEDGADGPRTRGSGGVARFRIPRGPVDVVADGPTTRGLISG
                     VLGGAIRARVYKFIAGLVMAHGLPALIEHLERGVREGVVLVRGDDLRDWVDREDLGEL
                     RLPRDRPARVLLLVHGTFSSTRGSFGGLCTGSGPDALRRMLAGYDAVFGVDHRSLSRA
                     PQDNAAAIRDLLLAHPWPHPPEVDIVCFSRGGLVTRELVERVLPGAGWQGTIGRVICV
                     ATTNAGTELARADNWRDLIDVYTTLACATMRGLGLAGGGLAALVGGEVVTAVGALVQA
                     LASGLLQDGTVPGLSAMDPEGEVVQALNARTLPAGPGPRYFAITSCFDASKPGAAGLT
                     AHAKRVLAGRVTGKLYRGAASDLVVHNASTHTLHPSRSYSDRHHFEANGSVYHVNFFL
                     QPEVVARLVAWLGLAEVQPARPPGFVIPALVRREAFEDQPLAFGPDDALVDLHVRAEA
                     PARVGLEELFTVHVELSREALALEAGVNAALASALVEASELVTVELVPRARVSPVAQL
                     PVSVAVPAQGGSVGLWFRLRAEEVGEGELWVIVRQRGRPLARLVLRVQVAEGTGVAAE
                     LVRAAAAPDRAAGVEPDAVLEVIELREAGGLRYRYHLRAAALGLHHRFESRLIPSGAD
                     DYVRGLFDRIEREWKSAGADPRAFDRRLRALGVQLGEQLLPPELMALLWDRREQLLGL
                     QLLSDEGRIPWELVHLKHPERPLAADQSAFLGQLGLVRALWGVALPQRLSARAGKAFT
                     VVPEYPTPALALPSALSERTWLSEHVGARPMDPRPEATLDALATPGSFDLLHFACHGE
                     VEAH"
     gene            5888..>6083
                     /locus_tag="VGB85_05310"
     CDS             5888..>6083
                     /locus_tag="VGB85_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133471.1"
                     /translation="MQPRLSVFVLPVLAVLTSCGDDIAADPTGTACTVTERVRLATPP
                     EGWVLQADDEYQLHRFPDFIL"
BASE COUNT          915 a         2423 c         1917 g          828 t
ORIGIN      
        1 cgtgcgcctc gacctcgccg tgacacgcga agtgcagcag gtcgaagctg cccggggtcg
       61 ccagcgcgtc gagcgtcgcc tccggccgcg ggtccatcgg gcgcgccccg acatgctccg
      121 agagccatgt cctttcagac agggccgagg gcagcgccag cgccggcgtc ggatactctg
      181 gcacgaccgt gaacgccttg cccgcgcgcg ccgacagtcg ctgcggcagc gcgacccccc
      241 acagcgcgcg caccagcccg agctgcccga ggaacgcgct ctgatcggcc gccagcggcc
      301 gctccgggtg cttgaggtgc accagctccc acgggatcct gccctcgtcg gacagcaatt
      361 gcagcccgag caattgttca cgccggtccc acagcagcgc catcagctcc ggcggcagca
      421 gctgctcccc gagctggacc ccgagcgcgc gcaggcgccg gtcgaaggcc cgtggatctg
      481 cgccggccga cttccactcg cgctcgatgc ggtcgaacag cccccgcaca tagtcgtccg
      541 cccccgaggg gatcagccgc gactcgaagc gatgatgcag cccgagcgcg gcggcccgca
      601 ggtgatagcg gtagcgcagc ccgccagcct cgcggagttc aatcacctcg agcaccgcgt
      661 ccggctcgac ccccgcggcc ctgtccggcg ccgccgcggc gcgcaccagc tcggccgcca
      721 cacctgtccc ttcggccacc tgcacacgca gcaccagccg cgcgagcggc cgcccgcgct
      781 ggcgaacgat cacccacagc tcgccctcgc cgacctcctc ggcgcgcagg cgaaaccata
      841 aaccaacgct cccaccctgc gccggcacgg cgaccgacac cggcagctgc gcgaccgggc
      901 tgacccgcgc gcgcggcacc agctcgaccg tcaccagctc gctcgcctcg accagcgcgc
      961 tcgccagcgc cgcgttgacg ccggcctcga gcgccagcgc ctcgcgcgac agctcgacgt
     1021 ggaccgtgaa cagctcctcg agcccgaccc gcgccggcgc ctcggcgcgc acgtgaaggt
     1081 cgaccagcgc gtcgtccggc ccgaacgcga gcggctgatc ctcgaacgcc tcgcgccgga
     1141 ccagcgccgg gatcacgaag cccggcggcc gcgccggctg cacctccgcg agaccgagcc
     1201 acgcgaccag cctggcgacc acctccggct gcaggaagaa attgacgtga tacaccgagc
     1261 cgttggcctc gaagtggtgc cggtcgctgt agctgcgcga cggatgcagc gtgtgcgtcg
     1321 acgcgttgtg caccaccagg tcgctcgccg ccccgcggta cagcttcccg gtcacccgcc
     1381 ccgcgagcac ccgcttcgcg tgcgccgtca gccccgcggc ccccggcttc gacgcgtcga
     1441 agcacgacgt gatcgcaaag taccgcggcc ccggccccgc gggcagcgtg cgagcgttga
     1501 gcgcctgaac gacctcgccc tcggggtcca tcgccgacag ccccggcacg gtcccgtcct
     1561 gcagcagccc gctcgccagc gcctgcacga gcgcgccgac ggccgtgacg acctcgccgc
     1621 cgacgagcgc cgcgagccca ccaccggcga gcccgagccc ccgcatcgtc gcgcacgcga
     1681 gcgtcgtata cacatcgatc aggtcgcgcc agttgtccgc gcgcgccagc tcggtgcccg
     1741 cgttggtggt cgccacgcag atcacccgcc cgatcgtccc ctgccagccc gccccgggca
     1801 gcacgcgctc gaccagctcg cgtgtcacca gcccgccgcg cgagaagcag acgatgtcga
     1861 cctcgggcgg atgcggccac gggtgcgcga gcagcaggtc gcggatcgcc gccgcgttgt
     1921 cctgcggcgc gcgagacagc gagcggtggt cgacgccgaa caccgcgtcg tagccggcca
     1981 gcatgcggcg cagcgcgtcc ggcccggagc ccgtgcacag cccgccgaag ctgccgcgcg
     2041 tcgacgagaa ggtcccgtgc acgagcaaca acacccgcgc cgggcggtcc cgcggcagcc
     2101 gcagctcccc gagatcctca cggtcgaccc agtcgcgcag gtcatcaccg cggaccagca
     2161 ccacgccctc ccgcaccccc cgctcgaggt gttcgatcaa ggccggtagt ccgtgcgcca
     2221 tcaccagccc ggcgatgaat ttatagacgc gcgcacggat cgcgccgccg agcaccccgg
     2281 agatcaggcc gcgcgtcgtc ggcccgtccg cgacgacgtc gaccggcccc cgcggaatgc
     2341 gaaagcgggc cacgccgccg ctgccacgcg tccgcggccc gtccgccccg tcctcgggca
     2401 agctccagca gaacatccca tcttgctcga gcagcacgac ggcgcgctcg tcctcctcga
     2461 tcgtgatgtc gaattcgagg gcgtcgtccg cagcgccatc gaggtcgatc gcgtcgagcg
     2521 agcgcgcacg cgcgccgctg tcgggctcga gcgtgatcgt gtcgatgacg accagcccct
     2581 gccgcgccag cgcctcgtcg agctgcccgg tgatcgcgac gtcctcggcg gcgtcgaggt
     2641 cgatcggcgc cgagaggtcg aggcgcccgc gcgtcaccgt ctcgtcgcgt ccgctctcgg
     2701 cgaggcgcat gccggccggc agcgaacgga tcctcaccgc gctggcgacc acctccggcc
     2761 gcgcagcgaa cgggtcctcg ccctcggccg gaaactcggc cccctcctcg gccgtcatcg
     2821 cctcgaagtc gatcacgtac gcgtccgggt cggcgaaggg cagcggctgg tcgagcaggc
     2881 gcgggtccca tgccggaggg atcggctggc cgtcgctgcg cgtgaagccg ggcccgccgt
     2941 tgccgagccc gagctcggtc tgcgcccaca cgcccgcgtg cagccagatc tctgcgcccg
     3001 ggcagttgct cttgccgcgc ttggcctggc gatgcgcgaa cacatcggtg atctcgccgc
     3061 cattgcgtgc gacctccgcg acgatccacc ggatcgcgcg gcgcaggccg tcgatcatcg
     3121 ccggcgtcag cgtcgacggc ccgcccccct gcttccagaa cgaggccggg cgcgccgggt
     3181 caccgatgaa attgcctgcc acctcgatcg ccaccgtgtc gcggttgagc gcgtccgacg
     3241 accacagata cgcctgcagc ggatgaatca ggtacacccg cccgtcgctg tgcacgccca
     3301 tgtgcgcggt cacccgcggc cacgcgcgcg ccccgaagcc gtgcaccccc gtctgatgca
     3361 gcgtgatccc cttgagctcg ctccacgccc gcgtccgctt gcgcttctcg cccgggctct
     3421 gcgccgtgac gtcgaagtcc ggcctgtcct cggggacagg ctcggtcgtg tcggccacca
     3481 ccgccgaccc ggcgccaccg ggtgccacga cacctgagac gctggacctc acggccggcg
     3541 gttcgtcgac cccggccgtc acgacgaccg cctcgagcac gagctccgcg atcgcgccag
     3601 cctgcggccg ctccggatcc gccgcgataa tggccgcctc ctcatcactc gcctcgtcac
     3661 cgccgtcagg cgcggccggc agctcgaccg cggcgcccgc ggtgaacgac ccgcgcgccc
     3721 tatttaaagt gtcaccatag ggcgggtcgt agctgccctt cttgacctgc cggcgccgcg
     3781 ccttgacctt ctcgataaac tccgtctcga gcgccggctc gcggtcccac gccgccgggt
     3841 cggcaacatc gacccccgaa tggctcgccg cgagctcttt caaaaattcc tcggaccacg
     3901 tacgcacata tgccggcatc cagttataaa gccgcgccat gatgccgagc ccgtaggtcg
     3961 agctacaaaa gctccgcagc ggctgcccgg ggaccttttc gacggtcacg acgacgcggt
     4021 cgccctcgcg cgtgcgatgc acgtggcggt ctgcgcgacc gagcagccgc gcgaggttct
     4081 tgccctctat taaattctcg ggccgggccg cccaggcctg ccaggcggct tcggagtccg
     4141 cgccggcgag cagcgtcgtc tcgctgcgcg acgcgccggt gccgtgcacc cgcaccacgc
     4201 ggtggctcgt gcgcccgccg atgcgcgtgg cgacggcgcg gtcgaggaac atctctttcc
     4261 agaacaggca ctgcccgacc tggacctcga cgtcattgcc ggccagcatc aacgcgccga
     4321 ccagcaccgg ctgagtccgc agatatgccc agccgcggtc accgcgatag acgaccccat
     4381 cggcctcgat cttgacgtgc gcgccggtcc gcgagatctc acagcgcgcc ttgcgatcga
     4441 agtagggata cggcccgtgc tcgacgtcga tccccgccgc caaaaagaac cgcgcgaacg
     4501 cccccggcgc gtgttccttc aaaaagatca ggaggcctgc gagcgacccg gcgtggccgg
     4561 cgaactgcgc catgccccac gatacgatct ggtcgtccca gctgttgacc gcgccgaacg
     4621 acccgcgaga ctctttgaat gcattctcgc ggaggatcgc gatctcgctg gcagagaagc
     4681 cccgcgcgat cagcatctcg tcgcgtagca ggtcgcggcg ggcgccgtcg taggccatca
     4741 cgttgaacgc gtcgcacagc gtcttgccgt cgacgccggt gcggtcgcgc gtcttgtagc
     4801 gaaacgacac gcgatcgccg tacgcctgca ccgtgtgatg ggcgccgtcc gtggtcgtca
     4861 gcgtgaacac gccgcgctgc gtgcccggct gcgccggcat cgactccggg acgaccacgg
     4921 ccggggtcgc cggcttcccc gtggcccgcg gcgggtgcgt cgagttagtg cacagctcga
     4981 gcgcggcgac cgtcgccggt ccatcgacgc cgtcgaccgt cagcgcccgc gccccgggcc
     5041 gaagccgccc gacctggcgc tggaacgccc ggatcgcctg ccgcgtcttc ccaccgggca
     5101 ccccgtcgac gacgagatcc ggcgccacca gccccttgag gcagctctgc agccagcgct
     5161 cgtagctgct gtcgtcgatc tcgatcagcg cgccgaacgc caggaagtcg agcgcgggac
     5221 cgtcgagctc ggcctcgccg tcgccctcgt cccactgcag gtcctccgcg tcgctccact
     5281 cgacatcgac gaactgctca taatccacag caaagctcgc gccatcggac tcgcacatcg
     5341 caccggtctg gtcctcttca accatcacgc accaccccgt cagctatgat caccgtcaga
     5401 tcgtatcgct cagatcgtcg tcctgcgcgt ccgcctcgac ataaccgtcc ccccggtcgt
     5461 ccgggtcctc cgcgaacgcg agcgcgccgt cgatccagcg cacgcgaaac agctctggcg
     5521 cccgaccgag gtcgccagct gaattgaaag atcgctggcg acgttcccaa ttctattaag
     5581 ggtgccgcgc gagatgacac cataatcgcc gatggcgacc tgcgccgtca ccggcagcca
     5641 cgccgcccgg cacccgagct cgcgccgcag gatggcatca tcctggcgtt caagactgcg
     5701 cataacaacc cccaaacgcg cccacgttat cggagcccgc cgtgatgcgc aaccgggccc
     5761 ctcccagacc agtttcgccc gccgtggccg cacccgcgct gtcttcaacg agtctgcgtg
     5821 gtatacctgc gcagatccga ccggccgccg gcagatcgac caccacaacc gcgcgatcga
     5881 ggagccgatg cagccacgcc tatccgtctt cgttcttccc gtcctcgccg tactaacctc
     5941 gtgcggcgat gacatcgccg ccgacccgac cggcaccgcg tgcacggtca ccgagcgtgt
     6001 gcgcctcgcc accccgcccg agggctgggt gctccaggcg gatgatgagt atcagctgca
     6061 ccgcttcccg gacttcatcc tct
//
LOCUS       DASWNJ010000295         2891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_6101, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000295 DASWNJ010000000
VERSION     DASWNJ010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6101"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="VGB85_05315"
     CDS             complement(<1..728)
                     /locus_tag="VGB85_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133472.1"
                     /translation="MSIRGRVTSLLCLALAACGDSGGATDGATAGASTTITTAATMPT
                     ASDPSGAVPTTGEGGSQSASEGATTEATSTTGATGTSDSTSGGDEGPKLDLGVPDGGA
                     ACGCEFSYVWVANSEESTVSKINMETLKEEGRYLTRADGIGNPSRTSVALNGDVAVAN
                     RHGGLVKFHADVGDCVERNGKPGIQTSSGKDDVLAWEDEECRAWYLDFPTSNQRPVAW
                     TQGTVTPGTCDTTGEKVWTVMSEK"
     gene            1080..2279
                     /locus_tag="VGB85_05320"
     CDS             1080..2279
                     /locus_tag="VGB85_05320"
                     /inference="COORDINATES: protein motif:HMM:NF024668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEY0133473.1"
                     /translation="MSSTFGDLREERERVTWKLLKDHHIPAGMETFPAADDRGWETIR
                     RAIDDSDYYVLIVGWRYGSIVPGTTLSWTHREYRYAKERNIPILAFLREEDATPYSKT
                     DHDAGAQVRAFREEIQSEKLYGSWRHGEDLCNAVGEALWRQIRNDEDEGTERPGWFRG
                     DAGIVSAETLDELARLSQENHSLRTQLDAVRLTEPAQLSLELPPSTQVLTFHPWQPWS
                     AAISLPTGPSTTTTVKADDFKRWVDALACTFWLPLTLVNTGRTTATDVLVDLTIPRCR
                     RILLERPEAGPITDTPSPVTLQRASNPGLDAYIQRVGPSGDGFEVRHRIKRVLGKSSE
                     HLVFFGAVLDDRLDWPSDSVIDVAFSIRDSTGVVHDGTVTTPIVILQGEPLNAVTAQA
                     FYARHGR"
     gene            complement(2369..>2891)
                     /locus_tag="VGB85_05325"
     CDS             complement(2369..>2891)
                     /locus_tag="VGB85_05325"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEY0133474.1"
                     /translation="AWQRATHRFGGHSHCDSRRSSCDSGRRWFGGRCGPGGGGPERGS
                     WGGGGGGGDEGGFDGGGNFGVRRPLRFLAYKLELDERQVAELAMVLDTVKTERAQAEV
                     DQRRTTAAYADLLSAETFDEAAATKVTDERVQSARHLQTAVQASLAKIHALLQPEQRR
                     RLTYLLRTGALRL"
BASE COUNT          462 a         1052 c          869 g          508 t
ORIGIN      
        1 ttcttctcgc tcatcaccgt ccacaccttc tcgccggtcg tgtcgcaggt gcccggcgtc
       61 accgtgccct gcgtccacgc gaccggccgc tggttcgagg tcgggaagtc gaggtaccac
      121 gcgcggcact cctcgtcctc ccacgccagc acgtcgtcct tgccgctcga cgtctggatc
      181 cccggcttgc cgttgcgctc gacgcagtcg ccgacatccg cgtggaactt caccagcccg
      241 ccgtgccggt tggcgaccgc gacatctccg ttgagcgcga ccgacgtgcg cgacgggttg
      301 ccaatgccgt ccgcgcgcgt caggtagcgc ccctcctcct tcaacgtctc catgttgatc
      361 ttcgagacgg tgctctcctc cgagttggcg acccacacgt aggagaattc gcagccgcac
      421 gccgccccgc cgtccggcac cccgaggtcg agcttgggcc cctcgtcgcc gccgctcgtc
      481 gagtcgctcg tgcccgtcgc gccggtcgtg ctcgtcgcct ccgtcgtcgc cccctcggac
      541 gccgactgcg acccgccctc gcccgtcgtc ggcaccgcgc ccgaggggtc gctcgccgtc
      601 ggcatcgtcg cggccgtcgt gatcgtcgtg ctcgcacccg ccgtcgcgcc atcggtcgcc
      661 ccgccgctgt cgccacaggc ggcgagcgcg agacacagga gagacgtgac acgaccgcgg
      721 atgctcattg tcgcaccata cgccgcgcgc ccccgcgccc gcaagctccg gcatcgatcg
      781 ctctcctcag cggcgtcttc tggctcgatc ttcttgctga gcgcgagctt tcggtccggg
      841 agggtcttac cactgccgct gccagacaca tgcgtggctc gtccttgacc gatggcgtca
      901 ccccgttgca ttccccttcg acgccgaccc agcagtgacg acgatcagcg accgtatgtt
      961 agcaaggctg aggatcgccc ttggctcacg ctgttgcacg acacgctcct gtacagccgg
     1021 ccgcaactcg ggtagcaaac tcaagcatgc gtcgtcggcg tccaaggtat caggtctttg
     1081 tgtcctccac ttttggagat ctccgcgagg agcgtgagcg agtcacctgg aagttgttga
     1141 aggatcatca catccccgcg ggcatggaga ccttcccagc ggccgacgac cgtggctggg
     1201 agacgatccg gcgggcgatc gatgactcgg actattacgt tctcatcgtg ggctggcgct
     1261 atggctcgat cgtcccagga acgacgctga gttggaccca ccgcgagtat cgctacgcaa
     1321 aggagcgcaa tatcccgatc ttggcgttcc tacgtgaaga agacgcgaca ccctactcga
     1381 agactgatca tgacgccggc gcccaagtcc gtgccttccg cgaagagatc cagtcggaga
     1441 agctctacgg ctcgtggcgg cacggcgagg atctgtgcaa cgcggtcggt gaggctctat
     1501 ggcgtcaaat ccgcaacgac gaggacgagg ggaccgagcg cccaggctgg ttccgcggtg
     1561 atgccgggat cgtctctgct gaaacacttg acgagctcgc acgcctctca caggagaacc
     1621 actcgctgag aactcagctg gatgccgtcc gactcacgga accggcgcaa ctctcactcg
     1681 agctgccgcc gtcgacccag gtactgacgt tccacccatg gcagccttgg tcggcagcga
     1741 tcagcctccc gaccggaccg tcaaccacta ccacggtgaa ggcagacgac ttcaagcgct
     1801 gggtggatgc cctcgcttgc acgttttggt tgccactgac actcgtcaac acaggacgca
     1861 ccaccgcgac cgacgtgctc gtcgacctga cgatcccccg ctgccggcgc atcctgctcg
     1921 aacgtcccga agccggcccc attactgata cgcccagtcc cgtcacactg cagcgagcct
     1981 ccaaccccgg tctcgacgcc tacatccagc gtgtgggacc gagcggtgat ggatttgaag
     2041 ttcgccacag gatcaagcgc gtcctgggca agtcaagcga gcacttggtc ttcttcggcg
     2101 cagtcctcga cgatcgcctg gactggccct cggacagcgt gatcgacgtc gcgttctcga
     2161 ttcgcgactc taccggcgtg gtccatgacg gcaccgtaac taccccgatt gtaatcttac
     2221 aaggtgaacc gttaaatgcg gtgaccgctc aagcttttta cgctcggcat ggtcggtgag
     2281 atccgacggc gaatgcgtgt accgcgcgaa gcggccaggt ccgcgagcga ctcgcggcct
     2341 ggcctatcgg tatcacgtcg tcacatgctc acaggcgcag ggcgccggtg cgcagcaggt
     2401 acgtcaggcg ccggcgctgc tccggttgca gcagcgcgtg gatcttcgcc agcgaggcct
     2461 gcaccgcggt ctgcaggtgc cgggccgact gcacgcgctc gtcggtcacc ttcgtcgccg
     2521 ccgcctcgtc aaaagtctcc gccgacagca ggtcggcgta ggccgcggtc gtgcgccgct
     2581 ggtcgacctc cgcctgggcc cgctcggtct tcaccgtgtc gagcaccatc gccagctcgg
     2641 cgacctggcg ctcgtcgagc tcgagcttgt acgccaaaaa ccgcagcggc cggcgcacgc
     2701 cgaagttgcc gccgccatcg aagccgcctt catccccgcc gcccccgccc ccgccccagg
     2761 agccgcgctc gggtccacca ccgccgggtc cgcagcgccc gccgaaccaa cgccggccgc
     2821 tgtcgcacga agatcggcga ctgtcgcagt gagagtgtcc accaaacctg tgcgtggcac
     2881 gttgccacgc c
//
LOCUS       DASWNJ010000296         1538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_146747, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000296 DASWNJ010000000
VERSION     DASWNJ010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146747"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="VGB85_05330"
     CDS             <1..1085
                     /locus_tag="VGB85_05330"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0133475.1"
                     /translation="PELAELLEQFMVVLRELGGGDQIREVGLRLAELYAERLADGDKA
                     RQILSELLETDADFVPAILALSTVEEQQGNDEGMRALLIRAQALQPQGVIGANLQLRL
                     AVFAETPEKRREHLETALHLHPANVEAARELLELSRKEGYWEQVAYLLALLAQYVEDD
                     AERKKLVLERVDILMTKVKDPEEALRALAPVYEAVQDDIEVNRRIADALFVSERYEEA
                     GGMYGWLVEVASAGPKRTKQQAHYLTRVARIEQHAGNTDKALERLKEAYKIDTTNAET
                     LVLLTEVYAQKGMWDDSLKTARAMLLQNVDQSGLVRRGDIYMHLAAAHVGLKEPQKAM
                     SMLKRGLEEDPKHPEIPARITALQAG"
     gene            complement(1247..>1538)
                     /locus_tag="VGB85_05335"
     CDS             complement(1247..>1538)
                     /locus_tag="VGB85_05335"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HEY0133476.1"
                     /translation="SACAGSGAPALEVCDQQDNDCDGVIDEDVPGTGTACTTGKPGVC
                     AQGVNRCLGGQMQCAANTQPSLEICNQKDDDCNNAVDDRCLSPEEAAKLKGG"
BASE COUNT          248 a          534 c          541 g          215 t
ORIGIN      
        1 ggcccgagct cgccgagctg ctcgagcagt tcatggtcgt gctgcgcgag ctcggcggcg
       61 gcgaccagat ccgcgaggtc ggcctgcgcc tcgccgagct gtacgccgag cgcctcgccg
      121 acggcgacaa ggcgcggcag atcctcagcg agctgctcga gaccgacgcc gacttcgtgc
      181 cggcgatcct cgccctctcg accgtcgagg agcagcaggg caacgacgag ggcatgcgcg
      241 ccctgctgat ccgcgcccag gccctgcagc cgcagggtgt catcggcgcc aaccttcaac
      301 tgcgcctcgc ggtgttcgcc gagaccccgg agaagcgccg cgagcacctc gagaccgcgt
      361 tgcacctgca cccggccaac gtcgaggccg cgcgtgagct gctcgagctg agccgcaagg
      421 agggctactg ggagcaggtc gcgtacctgc tcgcgttgct ggcccagtac gtcgaggacg
      481 acgccgagcg caagaagctg gtgctcgagc gcgtcgacat cctgatgacg aaggtcaagg
      541 acccggagga ggcgctgcgc gccctggccc ccgtctacga ggcggtccag gacgacatcg
      601 aggtcaaccg caggatcgcc gacgcgctgt tcgtcagcga gcgctacgag gaggccggcg
      661 ggatgtacgg ctggctcgtc gaggtcgcct ccgcggggcc caagcggacc aagcagcagg
      721 cgcactacct gacccgcgtc gcccgcatcg agcaacacgc cggcaacacc gacaaggcgc
      781 tcgagcggct caaggaggcc tacaagatcg acacgaccaa cgccgagacg ctggtcctgc
      841 tgaccgaggt ctacgcgcaa aagggcatgt gggacgactc gctcaagacg gcccgcgcga
      901 tgctgctgca aaacgtcgac cagtcgggcc tcgtgcgccg cggcgacatc tacatgcacc
      961 tcgccgccgc ccacgtcggc ctcaaggagc cgcaaaaggc gatgtcgatg ctcaagcgcg
     1021 gcctcgagga ggaccccaag caccccgaga tcccggcccg catcaccgcg ctgcaagccg
     1081 gctgatcgcc cggtcgcgtg aacgaaggcc cggccactgc ggtggtcggg cctctttgtt
     1141 tgtcgtcagg ctgacgatgc cgataacgca gagaagcccg gccaccatgc ggtggtcggg
     1201 cttcgttcgc tcgcacgacg gcgagcgggc agggcagggc agggcatcag ccgcccttga
     1261 gcttggcggc ttcctcgggc gacaggcagc ggtcgtcgac ggcgttgttg cagtcgtcgt
     1321 ccttttggtt gcagatctcg agcgagggct gggtgttggc ggcgcactgc atctggccgc
     1381 cgaggcagcg gttgacgccc tgggcgcata cgccgggctt gccggtggtg caggcggtgc
     1441 cggtgccggg cacgtcctcg tcgatcacgc cgtcgcagtc gttgtcctgc tggtcgcaca
     1501 cctcgagggc gggcgcgccg ctgcccgcgc aggcgctc
//
LOCUS       DASWNJ010000297         4996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_182378, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000297 DASWNJ010000000
VERSION     DASWNJ010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182378"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VGB85_05340"
     CDS             complement(<1..190)
                     /locus_tag="VGB85_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133477.1"
                     /translation="MSSGGVPQPVTGEFVARGGTGGMGSDLVGVALLGRYQVLEKIGD
                     GGMGTVYLAEHTTILKKFA"
     gene            complement(416..1882)
                     /locus_tag="VGB85_05345"
     CDS             complement(416..1882)
                     /locus_tag="VGB85_05345"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0133478.1"
                     /translation="MSRAPVLRTCLTASVLVAVAAATAALTVACSVVTKVDYAECEVS
                     ADCRAAFGLASVCGEDGLCETAKLDDRCKKVFPEDLFTAPEKYPDAVILGSLLDHNKE
                     SGDLLMVQAASLAVEGANVSGLTNGRKFGIVHCDYQEFENNSKTSEDAAIDGAKYLVD
                     VLGAVAIVGPGTSGLAEVVYTELQKPEHAPQALIVSPSATSVSLTNIDVRDGDKPGLF
                     WRTAPPDSQVGAELAGRMIGEGITTATVIYESGSYGEGLAIALQNGYEGQPKLPELLS
                     FTTADDIGPLVVQVSEGTPGAEVGVVFIGSEVPQVTDFLKRASVYPFYEGVTIFLGDT
                     AKAASVLEGTEAAANLYPRIRGVFPGTPQGPVFMTFRADYKAAFGPEPDSFAALTYDA
                     TWLALYGVAWAEFQRGGSLTGLDIGYGLQRVSYEKGMPIDVIRGTWTTVKSEFKAGRS
                     INITGVSGNLDYDPETEECTAPFSYWKIKDDGSGFEDI"
     gene            2295..>4996
                     /locus_tag="VGB85_05350"
     CDS             2295..>4996
                     /locus_tag="VGB85_05350"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0133479.1"
                     /translation="MDPRDRTLLHEAPSTMGVRGEGSDDISLARTAGGSGVTYSRVSE
                     EIGHGQLVGRYVVLNPLGAGGMGVVYAAYDPQLDRKVALKLLHIEAVSRSGVTQSRID
                     GHTRLLREAQAMARLRHPNVVAVHDVGSYGERVFIAMEFVEGSTLKSWLRSQPRTRKE
                     VLAVFVQAGRGLQAAHDAGLVHRDFKPENVLVSTSGQAQVLDFGLAKSTEDGADPPPG
                     QTPRGELELATSLNSLSRELTQQGAVMGTPAYMSPEQHMGLPTDGRTDQFSFCVALYE
                     ALYGTPPFPADSLPSLALAVVSGKVAEAPRESQARVPLWLRKVLLRGLSTDPAMRYAS
                     MAALLDALTQDPSSTRKRWLYAVAAVAALGLGVGGYAQYLAQSEQRCADAEQDLVGVW
                     DDDLRRTTREAFRAASEASFADDTWQRVSKTLDAYTSAWVQLQTAVCTATYIDHTQSV
                     ELLHLQSACLARRLSEVRAVTEEFAHPDAAVVERAVEIVAGLANLRACADEDALTSAV
                     MPPPDIDARAAVDAVRVRLDSAKMRERAGKYGAGLELARAATEDARSLAYPPVLAEAL
                     LVEADLLFRSGDTKAAETTLEAAIQAAAEGKHAAAGAEAWIALIRLVGAEQAQHQRAL
                     DLRLAADAAIAWAGGDPGLQARLFNTVGLVLHSQARYDAAIEASRRALALWEELPDDG
                     ETDARELAIADALSALGTAYNSKGEYEAADDYLRRVFASRRQTLGPDHPEVAASLHDL
                     GNNDYALGRYDDAEQHLGEALAIRTRVLGEQHPRVADTENSLGAVDYARGSFSAAREH
                     YARALAIREATLGPEHPNTASSWVNLGNADLMAGDLEKARANYERALAIQQKALGPDH
                     PNVAYSLTNIGLVLRNQGKFAESLAFYEKALKLRERAFGSENADV"
BASE COUNT          752 a         1871 c         1684 g          689 t
ORIGIN      
        1 ccgcgaactt cttgaggatc gtcgtgtgct cggcgaggta cacggtaccc atgccgccgt
       61 cgccgatctt ctcgagcacc tgatagcggc ccagcagcgc cacgcccacg aggtccgagc
      121 ccatgccccc ggtaccgccc cgcgccacga actcgcccgt gaccggctgc ggcacgccgc
      181 cggaggacac gatcgtccgc gctgggttgg atgtttcttg gcctgccacg tggatggacc
      241 cttcgcgctc ccgagcctac acgatcaccc cccatgctac gagccagccc ccacccgagt
      301 tacacgttcg gcgcgtcgct cgccgtcgcg cgaccgggaa atcaggtcat tgcgggtggc
      361 ttgaaactgg agccactgca agcccgggga gcccctggag gaccggtgcg tccgttcaga
      421 tatcctcgaa gcccgagccg tcgtctttga tcttccagta gctgaagggc gccgtgcact
      481 cctcggtctc cgggtcgtag tcgagattgc ccgagacacc ggtgatattg atgctgcggc
      541 cggccttgaa ctccgacttt acggtggtcc acgtgccgcg gatgacgtcg atgggcatgc
      601 ccttctcgta gctgacgcgc tggagtccgt agccgatgtc gaggccggtg aggctgcccc
      661 cgcgctggaa ctcggcccag gccacgccgt agagcgcgag ccaggtggcg tcataggtga
      721 gggccgcgaa cgagtcgggc tcgggtccga aggcggcctt gtagtcggca cgaaacgtca
      781 tgaagaccgg cccctggggg gtccccggga acacgccgcg gatgcgcggg tacaggttgg
      841 ccgcggcctc agtgccctcg agcacgctgg cagccttggc ggtgtcgccg aggaagatcg
      901 tcacgccctc gtagaacggg tagacgctgg cgcgtttgag gaagtcggtc acctggggga
      961 cctcggagcc gatgaagacg acgccgacct cggcgccggg ggtcccttcg ctgacctgaa
     1021 cgacgagcgg cccgatgtcg tcggcggtcg tgaagctgag cagctcgggc agcttgggct
     1081 ggccctcgta gccgttttgc agggcgatcg ccagaccctc gccgtacgag ccgctctcgt
     1141 agatgacggt cgcggtggtg atgccctcgc cgatcatgcg gccggcgagc tcggcgccga
     1201 cctgcgagtc cggcggagcc gtgcgccaga acaggcccgg cttgtcgccg tcgcgcacgt
     1261 cgatgttggt caggctgacg ctcgtcgccg aaggcgagac gatcagggcc tgcggcgcgt
     1321 gctccggctt ttgcagctcg gtgtacacca cctcggcgag gcccgaggtg ccagggccga
     1381 cgatggcgac tgcgccgagc acgtcgacga ggtacttcgc gccgtcgatc gcggcgtcct
     1441 cggacgtctt gctgttgttc tcgaactcct gataatcgca gtggacgatg ccgaacttgc
     1501 gcccgttcgt cagcccggag acgttggcgc cctcgaccgc gagcgacgcc gcctggacca
     1561 tcagcaggtc gccgctctcc ttgttgtgat cgagcagcga gccgaggatc accgcgtcgg
     1621 ggtacttctc cggggcggtg aacaggtcct ccgggaacac cttcttgcag cggtcgtcga
     1681 gcttggccgt ctcacacaga ccgtcctcgc cgcacacaga cgcgaggccg aacgccgcgc
     1741 ggcagtcggc gctgacctca cactccgcgt agtcgacctt ggtgacgacc gagcaggcga
     1801 cggtcaacgc ggccgtcgcc gccgcgacgg cgaccaggac agaggcggtg aggcaggtcc
     1861 gaaggacagg cgcgcgggac atcggcggca tattagtacc aagagcgcac cctgcctacc
     1921 cgcggtcgag cgccgggcgg ccgactcccc gcgccccggg aagcgactgc ggtcacgcag
     1981 agaccaattt acgggaccca ggcgagcaat caccacctgt agacaacgcc tcgccggcgg
     2041 tgagacgtcg ccatcgcggc cgatcgtcct atgtcccatc ggacatgttc gcatgcacag
     2101 gtcgcaaggg tcatgtcgaa gcgcgtcggg cgtgacgcgc cgcgggatcc gcagatcggc
     2161 gacgctcgtg cgcacgcgag ggcgtgctcg cagcgccggc tcgcacgcga tggcgtgctg
     2221 gagccggtcg cgcgaacgcg cccgcgctcg ccgtgatcgg cggccgcggg ccacggagtg
     2281 atacactccg cgcggtggat ccccgcgacc gcaccctcct gcacgaggcg ccctcgacga
     2341 tgggcgtgcg cggcgaggga tccgacgaca tcagcctcgc gcgcacggcc ggtggcagcg
     2401 gcgtcacgta ctcgcgggtg tcggaggaga tcggccacgg tcagctggtc ggtcgctacg
     2461 tcgtgctcaa cccgctcggg gccggcggca tgggggtcgt ctacgccgca tacgaccccc
     2521 agctcgaccg caaggtcgcg ctcaagctgc tgcacatcga ggccgtgtcg cgcagcggcg
     2581 tcacccagtc acgcatcgac ggccacaccc gcctgttacg tgaagcccag gcgatggccc
     2641 gcctgcgcca ccccaacgtc gtcgcggtcc acgacgtcgg ctcgtacggc gagcgcgtct
     2701 tcatcgcgat ggagttcgtc gagggctcga ccctcaagtc gtggctgcgc tcgcagcctc
     2761 gcacgcgcaa ggaggtcctc gcggtgttcg tgcaggccgg ccgcggcctg caggcggccc
     2821 acgacgccgg cctggtgcac cgcgacttca agccggagaa cgtgctcgtg tcgacctccg
     2881 gccaggccca ggtgctggac ttcgggctcg ccaaatccac cgaggacggc gccgacccgc
     2941 cgccgggcca gaccccgcgc ggcgagctcg agctggcgac cagcctcaac tcgctcagcc
     3001 gcgagctgac ccagcagggc gccgtgatgg gcacgcccgc gtacatgtcg cccgagcagc
     3061 acatgggcct gccgaccgac ggacgcaccg accagttcag cttctgcgtc gccctctacg
     3121 aggccctcta cggcaccccg ccgttccccg ccgactcgct gccctcgctc gcgctcgcgg
     3181 tcgtcagcgg caaggtcgcc gaggccccgc gcgagtcgca ggcccgcgtg ccgctgtggc
     3241 tgcgcaaggt gctgctgcgc ggcctctcga ccgaccccgc gatgcgctac gcgagcatgg
     3301 ccgcgctgct cgacgcgctg acccaggacc ccagctccac gcgaaagcgc tggctctacg
     3361 ccgtcgcggc cgtcgccgcc ctcggcctcg gcgtcggcgg ctacgcgcag tacctcgcgc
     3421 agtccgagca gcgctgcgcc gacgccgagc aggacctcgt cggcgtgtgg gacgacgacc
     3481 tgcgccggac cacgcgcgag gcctttcgcg ccgccagcga ggcgagcttc gccgacgaca
     3541 cctggcagcg cgtcagcaag accctcgacg cctatacctc cgcgtgggtc cagctccaga
     3601 ccgccgtctg caccgccacg tacatcgacc acacccagag cgtcgagctg ttgcatctcc
     3661 agagcgcctg cctcgcgcgc cgcctcagcg aggtccgggc cgtcaccgag gagttcgccc
     3721 accccgacgc cgccgtcgtc gagcgcgccg tcgagatcgt cgccggcctc gccaacctgc
     3781 gcgcctgcgc cgacgaggac gcgctgacca gcgccgtcat gccaccaccc gacatcgacg
     3841 cgcgcgccgc cgtcgacgcc gtgcgcgtgc gcctcgactc ggcgaagatg cgcgagcgcg
     3901 ccggcaagta cggcgccggc ctcgagctcg cccgcgcggc gaccgaggac gcgcgttcac
     3961 tcgcctaccc tccggtgctc gcggaggcgt tgttggtaga ggccgacctg ctctttcggt
     4021 caggtgacac caaggcggcc gagaccaccc tcgaggccgc gatccaggcc gccgccgagg
     4081 gcaagcacgc ggccgccggg gccgaggcct ggatcgccct gatccggctc gtcggcgccg
     4141 agcaggccca gcaccagcgc gcgctcgacc tgcgcctcgc cgccgatgcc gcgatcgcct
     4201 gggccggcgg cgacccgggg ctccaggccc gcctgttcaa caccgtcggc ctcgtgctgc
     4261 acagccaggc ccgctacgac gccgcgatcg aggccagccg gcgcgcgctc gcgttgtggg
     4321 aggagctgcc cgacgacggc gagaccgacg cccgcgagct ggcgatcgcc gacgccctct
     4381 ccgcgctcgg caccgcctac aacagcaagg gcgagtacga ggccgccgac gactacctgc
     4441 gccgcgtgtt cgccagccgc cggcagaccc tcggccccga ccacccggag gtcgccgcct
     4501 cgctgcacga cctcggcaac aacgactacg cgctcggccg ctacgacgac gccgagcagc
     4561 acctcggcga ggccctggcg atccgcacgc gcgtgctcgg cgagcagcac ccgcgcgtcg
     4621 ccgacaccga gaacagcctc ggcgccgtcg actacgcccg cggctcgttc tccgcggcgc
     4681 gcgagcacta cgcccgcgcc ctcgcgatcc gcgaggcgac cctcggcccc gagcacccga
     4741 acaccgcgag ctcgtgggtc aacctcggca acgccgacct gatggccggc gatctcgaga
     4801 aggcgcgcgc caactacgag cgggccctgg cgatccagca aaaggccctc ggccccgacc
     4861 accccaacgt cgcctactcg ctgaccaaca tcggcctggt cctgcgcaac cagggcaagt
     4921 tcgccgagag cctcgccttc tacgagaagg cgctcaagct gcgcgagcgg gcgttcggca
     4981 gcgagaacgc cgacgt
//
LOCUS       DASWNJ010000298         3135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_288427, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000298 DASWNJ010000000
VERSION     DASWNJ010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288427"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VGB85_05355"
     CDS             <1..447
                     /locus_tag="VGB85_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133480.1"
                     /translation="VVHDGANFIDLRVTHDEQEYVVGDTIAGYVPGSPIDLRAFGRTA
                     KFVDGDNRVLLILGTRVDAGDTSIDVLYSSEDATHFVKELQGKDHMRYIRPDSSFDRA
                     WSTAIDDLGPAGSYVIWAQFVPWHNPLYLVGNGQDMALTGAIRLQE"
     gene            complement(419..1576)
                     /locus_tag="VGB85_05360"
     CDS             complement(419..1576)
                     /locus_tag="VGB85_05360"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0133481.1"
                     /translation="MPSGPRLPLSDLRVVDLSRVLAGPYAAQILGDLGADVIKVEHPR
                     GDDTRRWGPPEHDGAAAYYLSANRNKRSRILDFTRPDDLAALLELLAGADVLIENYKT
                     GDLARHGLDFDALHARFPRLVYCSITGFGSTGPLAPRAGYDAVVQAMAGLMSVTGPDP
                     AHPTKVGVAVADLSTGLHAVIAILAALRERDRSGLGQRCELALYDTQVSGLANVAMNF
                     LCTGEVPRPLGDAHASIVPYQSFDSADRPLMLTVGNDAQFAALCQVLGEPQWAADPRF
                     TSNPARVAHRDVLVPAIAQKLSQKPRAHWLELLADTIVPHGPIQDLGELADDPQLRAR
                     DLFTTMDDGHTPCLRSPIVLSRTPISSYRRPPALGEHPDATWPTPEDGSHP"
     gene            1676..2308
                     /locus_tag="VGB85_05365"
     CDS             1676..2308
                     /locus_tag="VGB85_05365"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY0133482.1"
                     /translation="MKYTTKILLATALLAMNCTSELDGKPVAKVGEAKGDVKAATDVK
                     ADAPERTFKADPASSSIAFVGAKLTGDHKGDFKTFTGEATVAGTTPKSVKFSIDMGSV
                     TSDADDLTTHLKSPDFFAVEQFPKAEFSSTSVVAKAGGAANYEVTGDLDLHGVKKSIV
                     FPANITVDERGVKGTAEFKINRKDFKIEYPGKPDDLIKDEVLLKISLSFV"
     gene            2319..>3135
                     /locus_tag="VGB85_05370"
     CDS             2319..>3135
                     /locus_tag="VGB85_05370"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEY0133483.1"
                     /translation="MATLLISEDAMLAHDQGRGHPESPARLQAILARLRGRPVAGTRW
                     QAPQPATRARIARVHTPAYIDFVDEVRGQTIHIDEDVAMAPGSTDASYLAAGAAVDAV
                     TAVMQGAADNAFALVRPPGHHAEVDRAMGFCVFANVAIAAEHARQELGAQRVLIVDWD
                     VHHGNGTQHVFETRDDVLVFNAHQWPFYPGTGGLHEQGRGAGVGYTINAPLPAGMGDG
                     DYASLFRELLRPVAEIFKPDLVLVSAGFDAHRDDPLGGMTVTAEGFAGLCGVVR"
BASE COUNT          498 a          942 c         1229 g          466 t
ORIGIN      
        1 gtcgtgcacg acggcgcgaa tttcatcgat ctgcgggtca cgcacgatga acaggagtat
       61 gtggtcggcg acacgatcgc gggctatgtg ccgggtagcc cgatcgacct gcgggctttt
      121 ggcaggactg cgaagttcgt cgacggtgat aatcgtgtgc tgctgatcct cggcacgcgg
      181 gtcgacgccg gggacaccag tatcgatgtt ttatatagtt cagaggacgc gacgcacttt
      241 gttaaggagc tgcagggcaa ggaccacatg cgctatattc ggcccgactc gagcttcgac
      301 cgcgcgtggt cgacggcgat cgacgacctc gggccggcgg ggagctatgt aatttgggcg
      361 cagttcgtgc cgtggcacaa cccgctgtat ctcgtcggca acggccagga catggcgctc
      421 acgggtgcga tccgtcttca ggagtaggcc aggtcgcgtc ggggtgctcg ccgagcgcgg
      481 gcgggcggcg gtagctggag atcggtgtgc gcgacaggac gatcgggctg cgcaggcacg
      541 gggtgtggcc gtcgtccatg gtggtgaaaa ggtcgcgggc gcgcagctgc gggtcgtcgg
      601 cgagctcgcc gaggtcctgg atcgggccgt gtgggacgat cgtgtcggcg agtaattcga
      661 gccagtgggc gcgtggcttt tgggacagct tttgcgcgat cgcaggcacg aggacgtcgc
      721 ggtgggcgac gcgggccggg ttgctggtga agcgcgggtc cgcggcccac tgcggctcgc
      781 cgagcacctg gcacagcgcg gcgaactggg cgtcgttgcc gacggtgagc atcagcgggc
      841 ggtcggcgct gtcaaaactc tggtagggga cgatgctcgc gtgggcgtcg ccgagcgggc
      901 gcgggacctc gccggtgcac aggaaattca tggcgacgtt ggcgaggccg ctgacctggg
      961 tgtcatacaa ggcgagctcg cagcgctggc cgaggccgct gcggtcacgc tcgcgcaggg
     1021 cagcgaggat ggcgatgacg gcgtgcaggc cggtcgagag gtcggcgacg gcgacgccga
     1081 ccttggtcgg gtgcgcgggg tcggggccgg tgacgctcat caggccggcc atcgcctgga
     1141 cgaccgcgtc gtagccggcg cgcggggcga gcgggccggt cgagccgaag ccggtgatgc
     1201 tgcaatagac gaggcggggg aagcgggcgt gcagggcgtc gaagtcgagg ccgtgacggg
     1261 cgaggtcgcc ggttttataa ttttcgatca ggacgtcggc gccggcgagc agctcgagga
     1321 gggcggcgag gtcgtcgggg cgggtgaagt cgaggatccg cgagcgcttg ttgcgattgg
     1381 cgctcagata gtaggcggcg gcgccgtcgt gctcgggcgg gccccagcgg cgggtgtcgt
     1441 cgccgcgggg gtgctcgacc ttgatgacat ccgcgccgag atctccgagg atctgggcgg
     1501 cgtaggggcc ggcgaggacg cgtgagaggt cgaccacgcg caggtccgac aggggcagac
     1561 ggggtccaga cggcactgta cggcaggatg cgacgattgt tgccccgaat gcaacagcgt
     1621 gtggcggccc ccgggctgtc cccaggggcc ccggcggtgt accttctcgc cgatcatgaa
     1681 gtacacgacg aagatcctgc tggcgacggc gctgctggcg atgaattgca cgagtgagct
     1741 ggacggcaag ccggtcgcca aggtcggcga ggccaagggc gacgtgaagg cggccacgga
     1801 cgtgaaggca gacgcgccgg agcgcacgtt caaggcggac ccggcgagca gctcgatcgc
     1861 gttcgtcggc gcgaagctga cgggcgacca caagggcgac ttcaagacgt tcacgggcga
     1921 ggcgacggtc gcgggcacga cgccgaagtc ggtcaagttc tcgatcgaca tgggctcggt
     1981 gacctcggac gcggacgacc tgacgacgca cctcaagagc ccggacttct tcgcggtcga
     2041 gcagttcccg aaggcggagt tctcgtcgac ctcggtggtg gcgaaggcgg gcggcgcggc
     2101 gaactacgag gtgaccggcg acctcgacct gcacggggtc aagaagtcga tcgtgttccc
     2161 ggcgaacatc accgtcgacg agcgcggcgt gaagggcacg gccgagttca agatcaaccg
     2221 caaggacttt aaaattgagt acccgggcaa gcccgacgac ctgatcaagg acgaggtcct
     2281 gctcaagatc tcgctcagct tcgtgtgagg gggttggcgt ggcgacgctg ctgatcagcg
     2341 aggacgcgat gctggcccac gaccagggtc gcggccaccc cgagagcccg gcccggttgc
     2401 aggcgatcct cgcgcgcctg cgcggacggc cggtcgcggg cacgcgctgg caggcgccgc
     2461 agccggccac gcgcgcgagg atcgctcgcg tgcatacgcc ggcgtacatc gatttcgtcg
     2521 acgaggtgcg cgggcagacg atccacatcg acgaggacgt ggcgatggcg cccggcagca
     2581 ccgacgcgag ctacctcgcg gccggggcgg cggtcgatgc ggtgacggcg gtgatgcagg
     2641 gcgcggccga caacgcgttc gcgctggtgc gaccgcccgg gcaccacgcg gaggtcgacc
     2701 gcgcgatggg cttctgcgtg ttcgccaacg tggcgatcgc agccgagcac gcgcgccagg
     2761 agctcggcgc gcagcgggtg ctgatcgtcg actgggacgt acaccacggc aacggcacgc
     2821 agcacgtgtt cgagacccgt gacgacgtgc tggtgttcaa cgcgcaccag tggccgtttt
     2881 accccggcac cggcgggctg cacgagcagg gccgcggcgc aggggtcggc tacacgatca
     2941 acgcgccgct gccggccggc atgggcgacg gcgactacgc gagcctgttc cgcgagctgc
     3001 tgcggcccgt ggccgagatc ttcaagccgg acctggtgct ggtctcggcc ggcttcgacg
     3061 cccaccgcga cgacccgctc ggcggcatga ctgtcacggc ggagggcttc gccgggctgt
     3121 gcggcgtggt ccgcg
//
LOCUS       DASWNJ010000299         1581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_536251, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000299 DASWNJ010000000
VERSION     DASWNJ010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536251"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1472
                     /locus_tag="VGB85_05375"
     CDS             <1..1472
                     /locus_tag="VGB85_05375"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY0133484.1"
                     /translation="PGTMNPTQHGEVFVTDDGAETDLDLGHYERFVSHRMSRHSNYTS
                     GQIYESVIRKERRGEYLGQTVQVIPHVTNEIRSCILAAAEGLDLLIVEIGGTVGDIES
                     LPFLEAIRQLRLQLEPRDTVFVHLTLLPYIKTAGEIKTKPTQHSVMKMREIGIQPDIL
                     VCRTEAPFSRATRDKLAMFTNVRPDSVMSLPDQDSIYKVPLVLQAEGLDDKICELLNI
                     WSREPQLAEWKQLVHTIENPQQRVSIGVVGKYVDLAESYKSLHESLIHGGLANNAAVD
                     IVYVDAEQVQAGGADAQLGRLGGILVPGGFGERGSEGKIAAIRFARERQIPFFGICLG
                     MQMAVCEYARSFAELPNAHSSEFDPNLDGAVIALMAEQEGLKDMGGTMRLGAYDCELA
                     PGSLVARIYGKLRISERHRHRYEFNNRYRERLERAGLRLSGVSPDGTLVEMIELAPEV
                     HPFFVGCQFHPEFKSRPTAPHPLFSAYIAASLQYQRTRT"
BASE COUNT          265 a          575 c          520 g          221 t
ORIGIN      
        1 acccggggac gatgaacccg acccagcacg gcgaggtctt cgtcactgac gacggcgccg
       61 agacggacct cgacctcggg cactacgagc gcttcgtgtc gcaccgcatg tcgcggcaca
      121 gcaactacac ctcgggccag atctacgagt ccgtgatccg caaggagcgg cgcggcgagt
      181 acctcggcca gaccgtgcag gtcatcccgc acgtcaccaa cgagatccgc agctgcatcc
      241 tcgcggccgc ggagggcctc gatctgctga tcgtcgagat cggcggcacg gtcggcgaca
      301 tcgagagctt gccgttcctc gaggcgatcc ggcagctgcg cctgcagctc gagccgcgcg
      361 acaccgtgtt cgtgcacctg acgctgctgc cctatatcaa gaccgccggc gagatcaaga
      421 ccaagccgac ccagcactcg gtgatgaaga tgcgcgagat cgggatccag cccgatatcc
      481 tcgtgtgtcg caccgaggcg ccgttctccc gggccacgcg cgacaagctg gcgatgttca
      541 ccaacgtgcg ccccgacagc gtgatgtcgc tgcccgacca ggactcgatc tacaaggtgc
      601 cgctggtgct gcaggccgag ggcctcgacg acaagatctg cgagctgctc aacatctggt
      661 cgcgcgagcc gcagctggcc gagtggaagc agctcgtgca cacgatcgag aacccgcagc
      721 agcgcgtcag catcggcgtg gtcggcaagt acgtcgacct cgcggagagc tacaagagcc
      781 tgcacgagtc gctgatccac ggcggcctcg ccaacaacgc cgcggtcgac atcgtctacg
      841 tcgacgccga gcaggtccag gccggtggcg cggacgcgca gctcgggcgc ctcggcggga
      901 tcctcgtgcc gggcggcttc ggcgagcgcg gcagcgaggg caagatcgcc gcgatccggt
      961 tcgcccgcga gcgccagatc ccgttcttcg gcatctgcct cggcatgcag atggcggtct
     1021 gcgagtacgc gcgcagcttc gccgagctcc ccaacgcgca cagcagcgag ttcgacccca
     1081 acctcgacgg cgccgtgatc gccctgatgg ccgagcagga gggcctcaag gacatgggcg
     1141 ggacgatgcg gctcggcgcc tacgactgcg agctggcgcc cggcagcctc gtcgcccgca
     1201 tctacggcaa gctgcggatc tcggagcggc atcgccaccg ctacgagttc aacaatcgct
     1261 accgcgagcg cctcgagcgg gccggcctgc gcctcagcgg cgtctcgccc gacggcaccc
     1321 tcgtcgagat gatcgagctg gcccccgagg tgcacccgtt cttcgtcggc tgccagttcc
     1381 acccggagtt caagagccgg ccgaccgcgc cgcatccgct gttctcggcc tatatcgccg
     1441 cctcgctgca gtaccagcgc acgcgcacct gagcgccggg ctgcccgggc gcggtccgcc
     1501 cggcgacccg gcaggcgcgg gaccgatctc tgccgacccc gccggatccc gcaaataacg
     1561 ccctgagggc gcgtcgcggg g
//
LOCUS       DASWNJ010000300         1526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_606409, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000300 DASWNJ010000000
VERSION     DASWNJ010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606409"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..>1526)
                     /locus_tag="VGB85_05380"
     CDS             complement(206..>1526)
                     /locus_tag="VGB85_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133485.1"
                     /translation="LTGWASAQALDRPLVFHEPVTSRCRADDPVLLAADAAADAAAQE
                     PDAEAAVRGGLQAFLQIEGSACAGQSRAGGTLRAALARLDSQRLGEHGSALRDVAVAG
                     DTRVAALDEDGGVMLWDRQGPGRPLAGVRSGQAIARSPDGRWLAVGEVDGQLRVWDLN
                     DEAAAPRVQVHGARAVDVLAFARDGRLVSGDSEGRLKVWQLDGAAWRSTAELRAWAGV
                     ARVELAGGSLLAFGAGRAVVWQVERVDARPLVLTTGAALTAAALDVRGTQVVVGDAGG
                     VVTRWQLGRRGRVETLTAHAAAVHAVAWVGDAVASVGADDALRVAELGRRVRRAGPPL
                     VLVADTPVPVDSLVVTGGGRWLVGAGSEGAVVTWDLQQRSRRLPATLRPGHRGAIAAI
                     VAGDGWVVTAGADGVARAWDMSRETDSGTTLKADACRALGWSEPGCA"
BASE COUNT          212 a          675 c          477 g          162 t
ORIGIN      
        1 cggcgtcccc gagcccgacc tcgcgcgcca gatccagcac cccgtccgcg gccgggccgc
       61 gctcgtcaag aacgccgacg gccacacgca tcacatgcac gtccgctttc gctgccccac
      121 cggcgaaccc gactgcatcc ccgcgccctg atcccgccgc ccgcggccat acggctcgcg
      181 ggtatttctc catgcgccgt ccgtgtcagg cgcagccggg ctcggaccag ccgagcgcgc
      241 gacaggcgtc cgctttcagc gtcgtaccag aatctgtctc ccgggacatg tcccacgcgc
      301 gcgcgacccc gtcggcgccg gcggtcacga cccagccgtc gcccgcgacg atcgccgcga
      361 tcgcgccgcg atgacccggg cgcagcgtcg ccggcaaacg gcggctgcgc tgctgcaagt
      421 cccaggtgac gaccgcgccc tcgctgccgg cgcccaccag ccagcgcccg ccgccggtca
      481 ccacgaggct gtccacaggg acaggtgtgt cggcgacgag caccagcggc gggccggcgc
      541 gtcgcacccg gcgtccgagc tcggcgaccc gcagcgcgtc gtcggccccg accgaggcca
      601 ccgcatcacc gacccacgcg accgcatgca ccgcagcggc gtgggccgtc aacgtctcca
      661 cgcggccgcg ccggccgagc tgccagcggg tgacgacccc accggcgtcc ccgacgacga
      721 cctgcgtccc gcgcacgtcg agcgctgcgg ccgtgagcgc cgcccctgtc gtcaatacga
      781 gcggcctcgc gtcgactcgc tcgacctgcc acaccaccgc gcgcccggcc ccgaacgcga
      841 gcagagatcc tcctgcgagc tcgacccgtg ccaccccggc ccaggcccgc agctcggccg
      901 tcgaacgcca cgcggctcca tcgagctgcc acaccttgag tcgcccctcg ctgtcaccgc
      961 tgacgagacg accatcacgc gcgaaggcca gcacatcgac ggcccgcgca ccgtgcacct
     1021 gcacccgcgg cgccgcggcc tcatcattca gatcccacac acgcagctgc ccgtcgacct
     1081 ccccgaccgc gagccagcga ccgtccgggc tgcgcgcgat cgcctggccc gatcgcacac
     1141 ccgcgagcgg ccgccccgga ccctgccggt cccacaacat cacgccgccg tcctcgtcga
     1201 gcgccgcgac ccgcgtatcg ccagcgacgg ccacgtcgcg cagcgccgac ccgtgctcac
     1261 cgagccgctg gctgtccagg cgcgcgagcg cggcccgcag cgtccccccg gcccgcgact
     1321 gcccggcgca ggcgctccct tcgatctgca aaaatgcctg gagaccgccg cgcaccgccg
     1381 cctccgcgtc cggctcctgc gcggcagcgt ccgccgccgc gtcggccgcg agcaacaccg
     1441 ggtcgtctgc gcgacagcgg ctggtcaccg gctcgtgaaa caccagcggc cggtcgagcg
     1501 cctgcgccga ggcccacccg gtgagc
//
LOCUS       DASWNJ010000301         4577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_146803, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000301 DASWNJ010000000
VERSION     DASWNJ010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146803"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="VGB85_05385"
     CDS             <1..902
                     /locus_tag="VGB85_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133486.1"
                     /translation="SQSGAVVLGRLGGVGPQVRGTTAYFTIDSDLHARDLRSGTSIVL
                     QEDITGFTVSDTHVLWRAATGVSVTPMHLHELATGVDVPLGDYDGAVDHASLGEVPGQ
                     PWSWNFDASGSHVLHVPRDPDSPRAAFDLRGNLLSLPLPGDVFLTLASGHVLTRDPST
                     LEVFAARPGDSAPARLDHPIMNDTPQTLAVVGDRLESVFADALHEVPLDGGPTRLLVE
                     DVGVDRHWLGDRHLVTIFAGTLTAIDTQTGTRTIHGEHVTHMQAAHDDRGVYFNVAAA
                     PGDRANGLWYLPRSALDSGDVAE"
     gene            913..1512
                     /locus_tag="VGB85_05390"
     CDS             913..1512
                     /locus_tag="VGB85_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133487.1"
                     /translation="MLIRRSLGACVLLLLACGPKDEGSDDGSSDSSDTTAGSTMGGST
                     SPTTSGDTTGECGPVPTPTACTPDESSDSRIAVCAERDEAGCPGPIDQAGAECVWIAT
                     ASFAHDTTTCEAPKPGGACVAVEYYGDGCAGAACGSEDIPGDIFYRTNAACQTEVTLD
                     AFCGYSVIGWNACAWENRATDSCALPHPTKGPEPCNCAC"
     gene            1571..2977
                     /locus_tag="VGB85_05395"
     CDS             1571..2977
                     /locus_tag="VGB85_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133488.1"
                     /translation="MRVLRLVVFAALACFSACADDPTVCSGSEPTRLLAPEAFPYVNQ
                     LPVRAGDYTFMRAVNARGYQELLAGPGCGAEAVSIVEGILLAPFRYHDDPRDDDPTIA
                     CDTIGGHFFRVDPLGDSAPTLMLPELRCTAAATTEHGLLVVGNHDRALWLLPDFPRTD
                     TARIVSDQLDLRATGLPIPIVDDTLWYLAADRTLHRQDLVTGADTHVLDQVAAFDVDR
                     THVLWREQTGAPVAPMHLLDLASGTSSYLGLYHEDEDQRFGVEPRRDRLWRDAWTLSR
                     GGSFVLHIPTAADVPMQAFDLEGRAAAFPPLGAPLHFLADGTVLLTADDALLAARPGS
                     APIILDAPGDALLPRDPPPDPPALELLVGSELRLVPLDGSPSRLIAREVGERWTWLDE
                     QHILTIRRHTLTTIQPTTGRRQDIAAHVFDFSIAPDGGVHYIVAYQPGYLDSGLWYAP
                     DAALRPPPTKCVNLRVCE"
     gene            2985..4349
                     /locus_tag="VGB85_05400"
     CDS             2985..4349
                     /locus_tag="VGB85_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133489.1"
                     /translation="MTHRARSLLCTLMIAACDDDAEIAAICEGDVAVQLIATATPPSG
                     VEITPARGSTMMYIEASNRGATHLGRGCGAKPVRVADGARMRVAAIHPDPADDDPTLG
                     CMFDDYHYFRLDPKGSAPPKLLLPWLSCVFATPTEHGVLVMEDFHGGSISSGWWHYPD
                     FPSASGALRIEAAGTEPQVRGRTLYYHYWKDSTLRTYDLVTQTSDILLSGVAAFGVSD
                     THVLWREEVTDGVAPMRLLDLATGARIELGVSDPAIDHPPESTSGWAWNFDARAEHVL
                     HVPGDPEAPREAFDLTGARRTFPMPGVPFMMLPGGHVLSIDTATVELFATRPGDASPA
                     RLDYRPGDDYRYALTVVGERIQAVVGNDLHDISLDGSPAIVLVRDVGPGWTWLGEHQL
                     VTAFDGELTAIDTRTGARAVHGEHALYLERAPDGGGVYYLVAAEPGDPRNGLWFLPVT
                     ALAP"
BASE COUNT          643 a         1780 c         1476 g          678 t
ORIGIN      
        1 cctcgcagtc gggcgccgtg gtgctcggac gactcggcgg tgttggaccc caggttcgtg
       61 gcaccaccgc atatttcacg atcgacagtg acctgcacgc gcgcgacctc cgaagcggca
      121 catcgatcgt gctgcaggaa gacatcacgg gcttcaccgt cagcgacacc cacgtgctct
      181 ggcgcgcggc gaccggcgtg tccgtcacgc cgatgcacct gcacgagctg gcgaccggcg
      241 tcgatgtccc gctcggcgac tacgacggtg cggtcgacca cgcgtcgctc ggcgaggtcc
      301 cggggcagcc ctggagctgg aacttcgatg cgtcgggcag ccacgtcctg cacgtcccgc
      361 gcgaccccga cagcccgcgg gcagccttcg acctgcgcgg caacctcctg tccttgccgc
      421 tgccgggcga cgtgttcctg acgctggcga gcggccacgt gctcacccgc gacccgagca
      481 ccctcgaggt gttcgccgcc cgccccggcg acagcgcccc cgcgcgcctc gatcacccga
      541 tcatgaacga caccccgcag acgctcgccg tggtcggtga tcgtctcgag tccgtgttcg
      601 ccgacgcgct gcacgaggtc ccgctcgacg gtggccccac gcgcttgctc gtcgaggatg
      661 tcggcgtcga ccgtcactgg ctcggcgacc gccacctcgt cacgatcttc gccggcaccc
      721 tgaccgcgat cgacacgcag acaggaacac gaacgatcca cggcgagcac gtcacgcaca
      781 tgcaagcggc ccacgacgat cgcggcgtct acttcaacgt cgccgccgcg cccggcgacc
      841 gcgccaacgg cctgtggtac ctgccccgca gcgcgctgga cagcggcgac gtggccgagt
      901 aaccctgaaa aaatgctcat ccgacgaagc ctcggcgcct gcgtcctgct cctgctcgcg
      961 tgtggcccaa aggacgaagg ctccgacgac ggctcgagcg acagctcgga caccaccgcc
     1021 ggctcgacga tgggcggctc gacatcgccg accacctcgg gcgacacgac cggcgagtgc
     1081 ggtccggtcc ccacgcccac cgcctgcacc ccggacgagt cgagcgactc acgcatcgcc
     1141 gtgtgcgccg agcgggacga ggccggttgc cccggcccga tcgaccaggc cggtgccgag
     1201 tgcgtgtgga tcgccaccgc gagcttcgcc cacgatacga ccacctgcga ggcccccaag
     1261 cccggcgggg cctgtgtcgc cgtcgagtac tacggtgacg gttgcgccgg ggcggcctgc
     1321 ggcagcgaag acatcccggg cgatattttc taccgcacca acgccgcctg ccagaccgag
     1381 gtcaccctcg acgccttctg cggctacagc gtgatcggct ggaacgcctg cgcctgggag
     1441 aacagggcca ccgacagctg cgccctgccc cacccgacca aggggcccga gccgtgtaac
     1501 tgcgcgtgct gatccgcgcg ggcgtcgaag ttgtggcgcc cgtgagcgcc ggtggtaggc
     1561 tgacccgcga atgagggtcc tgcggctcgt cgtgttcgcg gcgctggcgt gtttctcggc
     1621 ctgcgcggac gatccgaccg tgtgctccgg cagcgagccg acgcgcttgc tggcgcccga
     1681 ggcgttcccg tacgtcaatc agctccccgt ccgcgccggc gactacacct tcatgcgggc
     1741 cgtgaacgcg cgcggctacc aggagctgct cgccggccct gggtgcgggg ccgaggccgt
     1801 gtcgatcgtc gagggcatct tactcgcgcc gttccgctat cacgacgatc cccgcgacga
     1861 cgacccgacg atcgcctgcg acaccatcgg cggccacttc ttccgcgtcg atcctctcgg
     1921 cgactccgcc cccacgctga tgctaccgga gctgcgttgc accgcggccg cgacgaccga
     1981 gcacggcctg ctcgtggtcg gcaatcacga tcgggccctg tggctgctcc ccgacttccc
     2041 gcggaccgac accgcgcgga tcgtcagcga tcagctcgac ctccgcgcca ccgggctccc
     2101 gatcccgatc gtcgacgaca ccctgtggta cctcgcggcg gaccgcacgt tgcaccgtca
     2161 ggacctcgtc accggcgccg acacccacgt gctcgatcag gtcgccgcgt tcgacgtcga
     2221 ccggacccac gtgctctggc gcgagcagac cggcgctccg gtggcgccga tgcacctgct
     2281 cgacctcgcc agcggcacca gcagctatct cggcctctac cacgaggacg aggaccagcg
     2341 cttcggcgtc gagcctcgca gggatcggct gtggcgcgac gcctggacgc tgtctcgcgg
     2401 tggttccttc gtgctgcaca tcccgaccgc ggccgacgtc ccgatgcagg ccttcgacct
     2461 cgagggtcgc gctgccgcct tccctcccct cggtgcgccg ctgcatttcc tcgccgacgg
     2521 cacggtcctg ctgaccgccg acgacgccct gctcgccgcg cgtcccggtt cggccccgat
     2581 catcctcgac gcccccggcg acgccctgct gccccgcgat cccccgccgg acccgcccgc
     2641 cctcgagctg ctggtgggct ccgagctgcg cctcgtcccg ctcgatggct caccgtcgcg
     2701 gctgatcgcc cgcgaggtcg gtgagcgctg gacctggctc gacgagcaac acatcctcac
     2761 gatccgcagg cacacattga cgacgatcca gccgaccacg ggccggcggc aggacatcgc
     2821 cgcccacgtg ttcgacttct ccatcgcacc cgacgggggc gtccactaca tcgtggcgta
     2881 ccaacccggg tacctcgaca gcgggctgtg gtacgcgccg gacgccgcgc tgcgtccacc
     2941 tccgaccaag tgcgtcaacc ttcgcgtctg cgagtgacat cgccatgacc caccgcgcac
     3001 gctccctgct ctgcacgctg atgatcgccg cctgcgacga cgacgccgag atcgcggcga
     3061 tctgcgaggg tgacgtggcg gtgcagctga tcgccaccgc gaccccgccc agcggggtcg
     3121 agatcacgcc cgcacggggc agcaccatga tgtacatcga ggcgtcaaac cgcggggcga
     3181 ctcacctcgg tcgcggctgc ggggcgaagc cggtgcgcgt cgcggacggc gcacgcatgc
     3241 gcgtcgccgc gatccacccc gatccagccg acgacgatcc cacgctcggc tgcatgttcg
     3301 acgactacca ctacttcagg ctcgatccga agggcagcgc gccgccaaag ctgctgctgc
     3361 catggctgag ctgcgtgttc gcgacgccca ccgagcatgg cgtgctcgtc atggaagact
     3421 tccacggtgg ctcgatatcc tcgggctggt ggcactaccc cgacttcccc tcggcgtccg
     3481 gcgccctgcg catcgaggcc gccgggacgg agccgcaggt tcgtggccgc acgctttatt
     3541 atcactactg gaaagacagc acgctgcgca cctacgacct cgtgacgcag acctcggaca
     3601 tcttgctctc cggtgtcgcg gcctttggcg tcagcgacac ccacgtgctg tggcgcgagg
     3661 aggtcaccga cggggtagcc ccgatgcgcc tcctcgatct cgccaccggc gcgcgcatcg
     3721 agctcggcgt ctccgatccc gcgatcgatc atccccccga gagcacctcg ggctgggcct
     3781 ggaacttcga cgcccgcgcg gagcatgtcc tgcacgtgcc cggcgacccg gaggctccgc
     3841 gcgaggcctt cgacctgacc ggcgcgcgtc ggaccttccc gatgcccggc gtccccttca
     3901 tgatgctccc gggcgggcac gtcctctcga tcgatacggc cacagtcgag ctgttcgcca
     3961 cgcgaccggg ggatgcctcg cccgcgcggc tcgactatcg gcccggcgac gactaccggt
     4021 acgcgttgac cgtcgtcggc gagcgcatcc aggcggtcgt gggaaacgac ctgcacgaca
     4081 tctcgctcga cggcagcccc gcgatcgtgc tcgtccgcga cgttgggccc ggttggacct
     4141 ggctcggcga gcatcagctc gtcaccgcgt tcgacggcga gctgactgcg atcgacacga
     4201 ggaccggcgc gcgcgcggtc cacggcgagc acgcgctgta cctcgagcgc gccccggacg
     4261 gcggcggcgt gtactacctg gtcgccgcgg agcccggtga cccgcgaaac ggcctgtggt
     4321 tcctgcccgt gaccgccctc gcgccctgac cgcggcggtg agccgacgct cgcgtcggcc
     4381 cacacgcccg cccccgtgcc gccgggcccg ccgtgctatg ccttggccgt ggccgtccat
     4441 ctgcctctct tgcccgccga gcctgccgcc agtcccgagc ccgcgccgcg ccgccgccac
     4501 ccggagtggc tgcgcgtgcc gatgcccggc ggcgacgggt atcaggcgct gcgtcgcatg
     4561 gtcaaggagc tcgggct
//
LOCUS       DASWNJ010000302         1640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_429822, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000302 DASWNJ010000000
VERSION     DASWNJ010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429822"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VGB85_05405"
     CDS             complement(<1..240)
                     /locus_tag="VGB85_05405"
                     /inference="COORDINATES: protein motif:HMM:NF024573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0133490.1"
                     /translation="MTDHPYLQRLRIKNYGCVQDCELKLTRLHALVGANDSGKSTLLR
                     GLGLAQRMFDVQQLLGPAPSNAAFELDWSDGLTARR"
     gene            complement(249..1499)
                     /gene="serA"
                     /locus_tag="VGB85_05410"
     CDS             complement(249..1499)
                     /gene="serA"
                     /locus_tag="VGB85_05410"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020797315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEY0133491.1"
                     /translation="MVNSDPTKSPATSFPKEKIKVLLLENIHESAHEMFSAEHFQVEG
                     LTTALSEAELAKRIADVHVLGIRSKTQVTPSVLAEARRLLSLGCFCIGTNQVALAAAN
                     ARGVPVFNAPLSNTRSVAEMILAQIVMLARQLGDRSTEVHQGIWKKVSNGCFEVRGKT
                     LGIIGYGHIGRQLGVLAEQLGLRVVFYDVASKLPMGNNRVRPTLSALLHEADFVTLHV
                     PATRDTNEMIGEKEFAQMKRGSYLLNASRGTVVHIPALVDALKSGHLAGAAIDVYPEE
                     PESNGKGFESPLRGLPNVVLTPHIGGSTVEAQAAIGREVATSLIKFVNTGATTGAVNF
                     PQIEMPQAPNTHRILNVHRNVPGVLRDVNAVVSARNANIHSQTLSTDAEIGYLIMDLD
                     QAVSGDVRDDIAALSNNIRTRILY"
BASE COUNT          238 a          514 c          590 g          298 t
ORIGIN      
        1 gcggcgcgcc gtcaggccgt cggaccagtc gagctcgaac gccgcgttcg acggcgcggg
       61 gccgagcagc tgctggacgt cgaacatgcg ctgcgccagc ccgaggccgc gcagcagcgt
      121 gctcttgccg ctgtcgttgg ctccgaccag cgcgtgcaag cgcgtgagct tgagctcgca
      181 gtcttgcacg cagccgtagt tcttgatgcg caggcgctgc aggtagggat ggtcggtcac
      241 gcgcgggctc aatagaggat gcgggtgcgg atgttgttgc tcaacgcggc gatgtcgtcg
      301 cgcacgtcgc cggagaccgc ctggtcgagg tccatgatca ggtagccgat ctcggcgtcg
      361 gtggagagcg tctggctgtg gatgttggcg tttcgcgccg acacgacggc gttgacgtcg
      421 cgcagcacgc ccggcacgtt gcggtgcacg ttgaggatgc ggtgcgtgtt gggcgcttgc
      481 ggcatctcga tctggggaaa gttgacggcg cccgtggtcg cgccggtgtt gacgaacttg
      541 atcagcgagg tggcgacctc gcgaccaatc gcggcctgcg cctcgaccgt gctgccgccg
      601 atgtgcggcg tgaggacgac gttggggaga ccgcgcagcg gcgactcgaa gcccttgccg
      661 ttggactcgg gctcctcggg gtagacgtcg atggcggcgc ccgccaggtg cccgctcttg
      721 agcgcatcga ccagcgccgg aatgtgcacg acggtgccgc ggctggcgtt gagcaggtac
      781 gagccccgct tcatctgcgc gaactctttt tcgccgatca tctcgttggt gtcgcgcgtg
      841 gccggcacgt gcagcgtgac gaagtcggcc tcgtgcagca gcgccgacag cgtcgggcgc
      901 acccggttgt tgcccatcgg cagcttgctc gcgacgtcgt agaacaccac gcgaaggccc
      961 agctgctcgg cgagcacgcc tagctggcgg ccgatgtggc cgtagccgat gatgccgagc
     1021 gtcttgccgc gcacctcgaa gcagccgttg ctgactttct tccagatgcc ctggtgcacc
     1081 tcggtcgagc ggtcgcccag ctggcgcgcg agcatcacga tctgcgcgag gatcatctcg
     1141 gcgacgctgc gcgtgttgct gagcggggca ttgaacaccg gcacgccacg cgcgttggcg
     1201 gcggcgagcg ccacctgatt ggtgccaatg caaaagcagc cgagcgacag cagccggcgc
     1261 gcctcggcca gcacggaagg cgtgacctgg gtcttgctgc gaatgccgag cacatggacg
     1321 tcagcgatgc gcttggcgag ctctgcctcg gacagcgcgg tggtcagccc ctccacctga
     1381 aagtgctcgg ccgagaacat ctcgtgcgcg ctttcgtgga tgttctcgag caggagaacc
     1441 ttgattttct ccttcgggaa cgacgtcgct gggctcttgg tgggatcgct gttgaccatc
     1501 atcgttgtcc tcgcttctac gcgaagccgc tctgagttca aggagcttgg ggaggtttag
     1561 gcgggcggtg acggccctgg tcgtgaaggc gccaggcgag ccacgagctg cccagccgct
     1621 gcccgaggtt gcgctcgcgc
//
LOCUS       DASWNJ010000303         2374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_321623, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000303 DASWNJ010000000
VERSION     DASWNJ010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321623"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VGB85_05415"
     CDS             complement(<1..466)
                     /locus_tag="VGB85_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133492.1"
                     /translation="MLLFTPVSFTGCAAPCTDDGLVWKQDSQECQQQLSASATDSSGT
                     VSATVTQGTMSAPTEGSASASESVSATEGSASMSASESATEAITEGSASVTETASDGG
                     GGACENGVKDPEETDVDCGGACGSTCEIGELCVDDVDCITLTCDGTACVPDPA"
     gene            complement(625..>2374)
                     /locus_tag="VGB85_05420"
     CDS             complement(625..>2374)
                     /locus_tag="VGB85_05420"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0133493.1"
                     /translation="TRFFADGVMIALCDEAAKLRGFGKVFKDATGEALAEESRRRHTE
                     QLRTLADVSSRLNAVLDLGSVLGVVTEEGRTLIGAHQAITNIITEGTGTLQSVALSDE
                     LRGAATAPFPPDRSAIYRAIYPASSAASKPVRLTQDELRAHAGWPGFHLAAESMPPMR
                     GLLAAPIVARGGDTLGVIQLSDKYDGDFTEEDEAILVQLAQMAAVAVENTQLYQALRD
                     ADRRKDEFLAMLAHELRNPLAPMQSALALLDAEGSRSPAAVRLRAVIGRQMSHMTRLV
                     DDLLDVARITSGKVVLKPESVELGRVVALAVETSRPLIDAKRHALTVDAPGPIWLDAD
                     PVRLAQVISNLLNNAAKYTDDGGQISVSARREGGDAVIRVRDTGIGLSADMLPRVFEL
                     FTQASAGLARSAGGLGIGLTLVRKLVELHGGNVSVSSAGRGEGSEFVVRLPAGTGGAS
                     DDAPAETPRPEGPRRVLLVDDNPDALETMSMLLEMFGHEVATASDGVKAVEVARSFRP
                     EVMFLDLGLPGRDGYEVARILREEFPKGTLWLVALTGYGQDEDRRRTHDAGFDQHLVK
                     PVEPEALEAALQRHGR"
BASE COUNT          324 a          850 c          850 g          350 t
ORIGIN      
        1 acgcggggtc gggcacgcag gccgtaccgt cgcaggtcag cgtgatgcag tcgacatcat
       61 cgacgcacag ctcgccgatc tcgcaggtcg acccgcacgc gccgccgcag tcgacgtcgg
      121 tctcctcggg gtccttgacc ccgttttcac acgccccacc gccgccgtcg ctcgccgttt
      181 cggtgaccga cgccgagccc tcggtgatcg cctcggtcgc cgactcgctg gcgctcatgc
      241 tcgccgagcc ctcggtcgcg gacacggact cgcttgcaga cgccgagccc tcggtcggcg
      301 cggacatcgt gccctgcgtg accgtggcgc tgaccgtccc actgctgtcc gtcgccgagg
      361 cgctgagctg ctgctggcac tcctggctgt cttgtttcca gacgaggccg tcatccgtgc
      421 agggcgccgc gcagcctgtg aacgagacgg gcgtgaacag gagcacccac gccagggtgc
      481 gagacggggg tccactcgag cgttgcgtca tccatcgacc tcggatggtc cagggacagc
      541 gacgccccgg tgagattcca aggcgacggc gatcgggcgc cgccgtctgc acgcgcgggc
      601 tcgcctcgat cgcgcgtcgc gggcctagcg accgtgacgc tgcaacgcgg cctcgagggc
      661 ctcgggctcg accggcttga cgaggtgctg atcgaagccg gcgtcgtggg tccgcctgcg
      721 gtcctcgtcc tgcccgtagc cggtcagcgc gaccagccac agcgtcccct tcgggaactc
      781 ctcgcgcagg atgcgggcga cctcgtagcc gtcgcggccg ggcagaccga ggtcgaggaa
      841 catcacctcg ggccggaagc tgcgcgcgac ctcgacggcc ttcacgccgt cggacgcggt
      901 cgcgacctcg tggccgaaca tctcgagcag catcgacatc gtctcgagcg cgtcggggtt
      961 gtcgtcgacc agcagcacgc gccggggacc ctcgggcctg ggagtctcgg cgggcgcgtc
     1021 gtcgctggcg ccgccggtgc cggccgggag gcggaccacg aactcgctgc cctcgccgcg
     1081 gcccgcgctg ctgacgctga cgttgccgcc gtgcagctcg acgagcttgc ggaccagcgt
     1141 gaggccgatg ccgaggccgc cggcggaccg cgcgaggccg gcgctggcct gcgtgaacag
     1201 ctcgaacacg cggggcagca tatctgcgga caaaccaata cctgtatcac ggacgcggat
     1261 cactgcatca ccaccttccc ggcgcgcgct gacggagatc tggccgccgt cgtcagtata
     1321 cttcgcagca ttgttgagga gattcgagat gacctgggcg aggcggaccg gatcggcgtc
     1381 gagccagatc ggcccgggcg cgtcgacggt cagcgcgtgg cgcttggcgt cgatcagcgg
     1441 gcggctggtc tcgaccgcca gcgcgaccac gcgcccgagc tcgaccgact cgggcttgag
     1501 caccaccttg ccgctggtga tccgcgcgac gtcgaggagg tcgtcgacca gccgcgtcat
     1561 gtggctcatc tgccggccga tgacggcgcg caggcggacc gccgcggggc tgcgcgagcc
     1621 ctcggcgtcg agcagcgcca gcgcgctctg catcggcgcc agcgggttgc gcagctcgtg
     1681 ggcgagcatc gccaggaact cgtccttgcg gcggtcggcg tcgcgcagcg cctgatacag
     1741 ctgcgtgttc tcgacggcga ccgccgccat ctgggccagc tgcacgagga tcgcctcgtc
     1801 ctcctccgtg aagtcgccgt cgtacttgtc ggacagctga atgacgccga gcgtgtcgcc
     1861 gccgcgggcg acgatcggcg cggccagcaa gccgcgcatc ggcggcatgc tctcggccgc
     1921 caggtgaaag ccgggccagc cggcgtgggc gcgcagctcg tcctgggtga gccgcaccgg
     1981 cttgctggcg gccgagctcg cggggtaaat cgcgcggtag atcgccgagc ggtcgggcgg
     2041 gaacggggcc gtcgcggcgc cgcgcagctc gtcggacagc gcgaccgact gcagcgtccc
     2101 ggtgccctcg gtgatgatgt tggtgatcgc ctgatgcgcg ccgatcagcg tgcggccctc
     2161 ctcggtgacg acaccgagca ccgagccgag gtcgagcacc gcgttgaggc ggctcgagac
     2221 gtcggcgagg gtgcgcagct gctcggtgtg ccgccgccgg ctctcctcgg cgagcgcctc
     2281 gccggtcgcg tccttgaaca ccttgccgaa gccacgcagc ttggcagcct cgtcacacag
     2341 cgcgatcatc acgccgtcgg cgaagaagcg cgtg
//
LOCUS       DASWNJ010000304         3130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_6217, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000304 DASWNJ010000000
VERSION     DASWNJ010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6217"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VGB85_05425"
     CDS             complement(<1..593)
                     /locus_tag="VGB85_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133494.1"
                     /translation="MTAPPDMSDRTSRLRRTAAAIDDWWRADAPAQRLAALRLLVGGF
                     ATIYLVARVVHLISFVDFPARNFAPVGVVSLLSAPLPAPLVIAAVILAIALAPAFTLG
                     WRFRVTGPAFAFMLLWVLSYRNSWGMVFHTENLLVIHALVLGLTRSADAWSLDVRRAA
                     IAPQPRAYGWPIRLLALVTVATYVLAGVAKLRLSGVA"
     gene            complement(590..1027)
                     /locus_tag="VGB85_05430"
     CDS             complement(590..1027)
                     /locus_tag="VGB85_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133495.1"
                     /translation="MYAWALGAVMLALVLRPLAQPAGADSFPFSSYPMFAFGRPDAIT
                     DVHHIVAFDAAGVGRPVPPRLVANDEVLQAEVSLLRAVRGGRKTSAAMCEKVAARLAG
                     DPAWADVDHLELLSERFDALRYFAGDTAPIKARRRARCTVPRT"
     gene            complement(1038..>3130)
                     /locus_tag="VGB85_05435"
     CDS             complement(1038..>3130)
                     /locus_tag="VGB85_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133496.1"
                     /translation="WRATAAAAAEALPHPSGDPAALTAELQATLASRPVPVPKDRPKF
                     RLWTAEPTPCAAAIDRPTLIVTYPVRADVPQAAGKPAAPVLRGVTRCVQAERAEDLMP
                     ALAATLTDAAAPGPMKIDVLTGTQALASSSPGPLSLLLRPGLDGVCDGPRCLMPWQLV
                     ALGQFNTYTPLPFIEDLRFGSDPTALRKVLAARDETTTEPPPETLDGLTRVTTESFIV
                     DRRGKVTHAPAMREPLTELTGDRLARAAIAAERHILAAQHKDGRFRYILQPFTGKVTW
                     RGFAVPRQAGTTLSLCELGTPTAAVKTAAARSLAMMASLERRSGPVSGLVYDEPKRSP
                     RRHAGLGDTALPLIAFLTCRPRTGPEHDELIARMAGFLLAMQREDGGFHPRFDLDAGA
                     PTPGPDPMYAAGQAVFALTLLEQLLASDAPPPGMPTQAEVHAAVERAMTYFSGDYWGH
                     DYYGFFFLEENWHCLAARAALPIHRHAGYERLCLDYVEFKQRLILDEASGVRDDLVGA
                     YGFGNVLPPHNTPTGGFGEALAAAMAVREAQGATRDADAALMHQVLAFLVEQQWTESN
                     CFACTPDQMVPGGYSESVGSPDMRIDYTQHVWSALGHGGRTLGLLRTSPTPTPVPSFS
                     TTPARPAKPRPAPSTTPTAKPTPSPSTTPTVKPTPSPSTTHKPTPSPSTTPSPAAKPS
                     PSTTPSPAEPRRGG"
BASE COUNT          441 a          988 c         1323 g          378 t
ORIGIN      
        1 caggcgacgc cgctgaggcg cagcttggcg acgccggcga gcacgtaggt ggcgacggtg
       61 acgagcgcga gcaggcggat cggccagccg tacgcgcgcg gctgcggggc gatcgcggcg
      121 cggcggacat cgagcgacca ggcgtcggcg ctgcgcgtca ggccgagcac gagggcatgg
      181 ataacgagca ggttctcggt gtgaaacacc atgccccagc tgttgcggta gctgagcacc
      241 cacagcaaca tgaaggcgaa ggcggggccg gtgacgcgaa agcgccagcc gagcgtgaag
      301 gccggcgcga gcgcgatcgc gaggatcacg gcggcgatca ccagcggggc gggcagcggg
      361 gcggacagca gcgagacgac gccgaccggc gcgaagttgc gggccgggaa gtcgacgaag
      421 ctgatcaggt gcacgacccg cgcgacgagg tagatcgtgg cgaagccacc gacgagcagg
      481 cgcagggcgg cgaggcgctg cgcgggcgcg tcggcccgcc accagtcatc gatcgcggcg
      541 gcggtgcggc gcaggcgaga tgtccgatcg gacatgtcgg gaggggccgt cacgtccgcg
      601 gcaccgtgca gcgggcccgc cggcgcgcct tgatcggggc ggtgtcgccg gcgaagtaac
      661 gcagggcgtc gaagcgctcg ctgagcagct cgaggtggtc gacgtcggcc cacgcggggt
      721 cgccagcgag gcgcgccgcg accttctcgc acatggcggc gctggtcttg cgaccgccac
      781 gcacggcccg gagcaggctg acctcggcct gcaggacctc gtcgttggcg accaggcgcg
      841 gcgggaccgg gcggccgacc ccggcggcgt cgaaggcgac gatgtggtgg acgtcggtga
      901 tcgcgtcggg gcgaccgaag gcgaacatcg ggtagctcga gaacgggaac gagtcggccc
      961 ccgcgggctg ggccagcggg cgcagcacga gcgcgagcat caccgccccg agcgcccagg
     1021 catacacggc accacggtca cccgccacga cgcggctccg cgggcgaggg cgttgtcgag
     1081 ggagagggct tggccgcagg cgagggcgtc gtcgagggcg atggcgtcgg cttatgcgtg
     1141 gtcgagggcg agggcgtcgg cttaaccgtg ggcgtcgtcg agggcgaggg cgtcggctta
     1201 gccgtgggcg tcgtcgaggg ggcgggcctg ggcttcgccg ggcgtgcggg ggtcgtcgag
     1261 aacgacggga ccggcgtcgg cgtgggcgag gttcgcagca ggccgagggt gcggccgccg
     1321 tggccgagcg cggaccacac gtgctgggtg tagtcgatgc gcatgtcggg ggagccgacc
     1381 gactcggagt agccgccggg gaccatctgg tcgggggtgc aggcgaagca gttgctctcg
     1441 gtccactgct gctcgaccag gaaggcgagc acttgatgca tcagcgcggc gtcggcgtca
     1501 cgcgtggcgc cctgggcctc gcgcacggcc atcgcggcgg cgagcgcctc gccgaagccg
     1561 cccgtcgggg tgttgtgcgg cgggagcacg ttgccaaacc cgtaggcgcc gacgaggtcg
     1621 tcgcgcacgc cgctggcctc gtcgaggatc aggcgctgct tgaactcgac gtagtcgagg
     1681 cacaggcgct cgtagccggc gtggcggtgg atcgggagcg cggcgcgggc ggccaggcag
     1741 tgccagttct cctcgaggaa gaagaagccg taatagtcgt gcccccaata gtcgccggag
     1801 aagtaggtca tcgcgcgctc gacggcggcg tggacctcgg cctgcgtcgg catgccgggc
     1861 ggcggggcgt cgctggcgag cagctgctcg agcaaggtca gcgcgaacac ggcctgcccg
     1921 gcggcgtaca tcggatcggg gccgggggtc ggcgcgccgg cgtcgaggtc gaagcgcggg
     1981 tgaaagccgc cgtcctcgcg ctgcatggcg agcaaaaagc cggccatgcg ggcgatcagc
     2041 tcgtcgtgtt cggggccggt gcgcgggcga caggtcagga aggcgatcag cggcaaggcg
     2101 gtgtcgccga ggccggcgtg ccgtcgcggc gaacgcttcg gctcgtcgta gaccaggccc
     2161 gagacggggc cggagcggcg ctcgaggctc gccatcatcg ccagcgagcg ggcggcggcg
     2221 gtcttgacgg cggcggtcgg cgtgccgagc tcgcacagcg agagcgtggt gccggcctgc
     2281 cgcgggaccg cgaagccgcg ccaggtgacc ttgccggtga agggctggag gatgtaacga
     2341 aaccggccgt ccttgtgctg cgcggcgagg atgtggcgct cggctgcgat cgctgcgcgg
     2401 gcgaggcggt caccggtcag ctcggtgagc ggctcgcgca tcgccggggc gtgggtgacc
     2461 ttgccgcggc ggtcgacgat gaagctctcg gtggtgacgc gggtcaggcc gtcgagggtc
     2521 tctggagggg gctcggtggt ggtctcgtcg cgggccgcga gcaccttgcg cagggcggtg
     2581 gggtcgctgc cgaagcgcag gtcctcgatg aagggcaggg gcgtgtacgt gttgaactgg
     2641 ccgagcgcga cgagctgcca gggcatcagg cagcgcggcc cgtcgcacac gccgtcgagg
     2701 ccggggcgca ggagcaggct cagcgggccg ggcgagctcg acgcgagggc ctgggtgccg
     2761 gtgagcacgt cgatcttcat cgggcccggc gcggccgcgt cggtgagcgt cgccgcgagc
     2821 gcgggcatga ggtcctcggc gcgctcggcc tgcacgcagc gggtcacgcc gcgcaggacc
     2881 ggcgcagccg gcttgccagc ggcctgcggg acgtcggcgc gcacggggta ggtgacgatc
     2941 aacgtcgggc gatcgatcgc ggcggcgcac ggggtcggct cggcggtcca caggcgaaac
     3001 ttcggcctgt ccttggggac aggtaccggg cggctcgcga gcgtcgcctg cagctcggcc
     3061 gtcagcgcgg ccgggtcccc ggacgggtgc ggcagcgcct cggccgcggc ggccgcggtg
     3121 gcgcgccaca
//
LOCUS       DASWNJ010000305         1517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_571077, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000305 DASWNJ010000000
VERSION     DASWNJ010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571077"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1517
                     /locus_tag="VGB85_05440"
     CDS             <1..>1517
                     /locus_tag="VGB85_05440"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEY0133497.1"
                     /translation="GSAGMSETGEPTTGPDEPPPPIEGYADLHLHMMAEAAFGGGWFH
                     GNAMGAEDIALAMCDGGFPGDHARARSDLTEQFTLCNDQSLPIDLATLAGQIPVLNAL
                     LFLGGIPASELLGKIEGTDGDTGLQQNRKNGLPDFSGWPRWDTIAHQQSWEGWLKQAH
                     GDGLRVVVMSAVSFDWLCTALPPGNLDRPQCDEMDDVRVQLEMANELDAANDWLEIAL
                     SAGHARKIASEGKLAMILSIEASSIFGDGDWLAQLDEVYALGVRTLQIVHQFDNRFGG
                     AAPHNTIFHVAQFTRNCHVDSDCGLTTPALTLGFDVDAECKNVQGLTDEGAALVDEMM
                     DRGMLIDAAHTSERTFSDIYALAEANTYYPLYMSHGHFREIMTPPKQREEKTTPVWIV
                     EALRKTGGVFGLRTAHEEVNAYPASSVANDCHGSTRSLAQAYAFGQLGLKVDIALGSD
                     FNGFIQQTRPRFGDNAACSASFADEATCQKALQELHGPPPLGSALDTQGFAHIGL"
BASE COUNT          256 a          582 c          474 g          205 t
ORIGIN      
        1 cggctcggcg ggcatgagcg agaccggcga gccgaccacc ggccccgacg aaccgccgcc
       61 gccgatcgag ggctacgccg acctgcacct gcacatgatg gccgaggccg cgttcggcgg
      121 cggctggttc cacggcaacg cgatgggcgc ggaggacatc gcgctggcga tgtgcgacgg
      181 cggcttccct ggtgaccacg cccgcgcccg ctccgacctc accgagcagt tcaccctgtg
      241 caacgatcaa agcctcccga tcgacctcgc cacgctcgcc ggccagatcc cggtgctcaa
      301 cgccctgctg ttcctcggcg ggatcccggc gtccgagctg ctcggcaaga tcgagggcac
      361 cgacggcgac accggcctgc aacagaaccg caaaaatggg ctgcccgact tctcgggctg
      421 gccgcgctgg gacacgatcg cccaccagca gtcctgggag ggctggctca agcaggccca
      481 cggcgacggc ctgcgcgtcg tcgtcatgtc cgccgtcagc ttcgactggc tgtgcaccgc
      541 gctgccaccg ggcaacctcg atcgcccgca gtgcgacgag atggacgacg tgcgcgtgca
      601 gctcgagatg gccaacgagc tcgacgccgc gaacgactgg ctggagatcg cgctctcggc
      661 cggccacgcg cgcaagatcg ccagcgaggg caagctcgct atgatcctca gcatcgaggc
      721 ctcgagcatc ttcggcgacg gcgactggct cgcgcagctc gacgaggtgt acgcgctcgg
      781 cgtgcgcacg ctccagatcg tgcatcagtt cgacaaccgc ttcggcggcg ccgccccgca
      841 caacacgatc tttcacgtcg cccagttcac ccgcaactgc cacgtcgaca gcgactgcgg
      901 cctgaccacc cccgcattga cgctcggctt cgacgtcgac gccgagtgca agaacgtcca
      961 gggtctcacc gacgagggcg ccgcgctcgt cgacgagatg atggaccgcg gcatgctgat
     1021 cgacgccgcc cacacctccg agcgcacctt ctcggacatc tacgcgctcg ccgaggccaa
     1081 cacgtattat ccgctctaca tgtcgcacgg ccacttccgc gagatcatga ccccgcccaa
     1141 gcagcgcgag gagaagacca cccccgtgtg gatcgtcgag gccctgcgaa agaccggcgg
     1201 cgtgttcggc ctgcgcaccg cccacgagga ggtcaacgcc taccccgcct ccagcgtcgc
     1261 caacgactgc cacggcagca cccgctcgct cgcgcaggcc tacgccttcg gccagctcgg
     1321 cctcaaggtg gacatcgcgc tcggcagcga cttcaacggg ttcatccagc agacgcgccc
     1381 gcgcttcggc gacaacgcgg cctgctccgc ctcgttcgcc gacgaggcga cctgccagaa
     1441 ggcgctgcag gagctgcacg gtcccccgcc gctgggcagc gcgctcgaca cccagggctt
     1501 cgcgcacatc ggcctgc
//
LOCUS       DASWNJ010000306         3181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_393122, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000306 DASWNJ010000000
VERSION     DASWNJ010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393122"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1187)
                     /locus_tag="VGB85_05445"
     CDS             complement(42..1187)
                     /locus_tag="VGB85_05445"
                     /inference="COORDINATES: protein motif:HMM:NF016883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytochelatin synthase family protein"
                     /protein_id="HEY0133498.1"
                     /translation="MQSLYRRPLPPALVAFAAPEGRAWFDAARAEGGMEAFFPLIEQF
                     HTQADPAFCGLGTLVVALNALGVDPGRLWKGPWRWFSEEMLDCCSPLERVRTQGITLD
                     ELACLARCNGAIAGLSRATADNVSTFRAALRGASRSAADPVLIASYDRSVLGQTGAGH
                     FSPIGGYHEASDHALVLDVARFKYPPHWVPVTALHAAMQANDPATGRARGWVELRRSP
                     RPASLLYTARCVGDGGFASVRAGLAVLREAVARAPTDTLPGALRAIFAAAQDIPVVVE
                     HRPLALAEHAVAADLLQAALRDTPVYRLVAAAAPPGWPAEAATLLALALPAELWDPLP
                     EPLRAAWLAVLDPERLPAAVRVESEHVHGQLADLCALTAAGSCQRCG"
     gene            1460..2671
                     /locus_tag="VGB85_05450"
     CDS             1460..2671
                     /locus_tag="VGB85_05450"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0133499.1"
                     /translation="MIEELRRDNAALRERLARVEPTAAKYEVLVSIMAEAVLVLDSDG
                     RYLTIEPTQGFPPEARAQLLGTCLPDGFGPEIGGQMVAAIRRSLATGEPGTIEFQVPL
                     PDRPLTVCAVFKPLTADTVLWYSNDITAERLLRQAEQGLRTFEAVVERTPDGVALITT
                     RGEVTYTNQAYRDLGGAFDPGDALLAARLAAPELVRAALVEATTHGHWTGRLELGVDA
                     GGRVPCQVSLVRLPEAPGRTAELAVIARDLRPLIEAEGQRLALQEQVIEAQRVALREL
                     STPLLPIATGVVCMPLIGTIDGPRAQEMMERLLDGIVRLRAHTAILDVTGVRVADAQV
                     ADGLLQVARAARLLGAHVILTGIGPDIAQTLVALHSELLGITTLGDLRSGIAHALAGR
                     GAPGSAARRRP"
     gene            2801..>3181
                     /locus_tag="VGB85_05455"
     CDS             2801..>3181
                     /locus_tag="VGB85_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133500.1"
                     /translation="MAADDIDARAFEPAGARWLARIHAALAGPGPAPTYLLTRFVLLR
                     VLGLVYLAAFLSALLQLVPLVGADGLTPAVDELAAHADAVGGAWSGFLERPTLFWLDC
                     SDGALWGVSLAGVLLSCVLLLGCEN"
BASE COUNT          406 a         1254 c         1114 g          407 t
ORIGIN      
        1 ggcatgcggg cccgcgaccg ccgtatgcag cgccgcgccg actagccgca gcgctggcag
       61 ctgccggcgg ccgtcagcgc gcacaggtcc gcgagctgcc cgtgcacgtg ctcgctctcg
      121 acgcgcaccg ccgcgggcag ccgctccggg tcgagcaccg ccagccacgc cgcacgcagc
      181 ggctccggca gcgggtccca cagctccgcc ggcagcgcga gcgccagcag ggtcgcggcc
      241 tcggccggcc atccgggcgg cgcggcggcg gcgaccagcc gatacaccgg cgtgtcgcgc
      301 agcgccgcct gcagcaagtc cgccgcgacg gcgtgctcgg cgagcgcgag cggccggtgc
      361 tcgacgacga ccgggatgtc ctgcgccgcc gcgaagatcg cccgcagcgc gcccggcaac
      421 gtgtcggtcg gcgcccgcgc gaccgcctcg cgcagcaccg cgagccccgc gcgcaccgac
      481 gcgaagccac cgtcgccgac gcagcgcgcc gtgtacagca agctcgcggg tcgcgggctg
      541 cggcgcagct cgacccatcc gcgcgcgcgt ccggtggcag gatcgttcgc ctgcatcgcg
      601 gcgtgcaacg ccgtcaccgg cacccagtgc ggcggatact tgaagcgcgc gacgtcgagg
      661 acgagcgcgt gatcactggc ctcgtggtaa ccgccgatcg gcgagaagtg acccgcgccg
      721 gtctggccga gcacgctgcg atcgtagctg gcgatcaaca ccgggtcggc cgccgagcgc
      781 gaggccccgc gcagcgccgc ccgaaatgtg ctcacattgt cggccgtcgc ccgcgagagc
      841 ccggcgatcg ccccgttgca gcgagccagg cacgcgagct cgtcgagcgt gatcccctgc
      901 gtccgcacgc gctcgagcgg gctgcagcag tcgagcatct cctcgctgaa ccaccgccac
      961 ggccccttcc acagccgccc gggatcgacg ccgagagcgt tgagcgcgac gaccagcgtg
     1021 ccgagcccac aaaacgccgg atcggcctgc gtgtggaact gttcgatcag gggaaaaaac
     1081 gcctccatgc cgccctcggc ccgcgccgcg tcgaaccacg cgcggccctc gggcgcggcg
     1141 aaggcgacca gtgcgggcgg caaggggcgg cgatagaggc tctgcatcgc cggcgatcct
     1201 gacacaacct cgacgtggtg gcagcccctg agcgacggtt cagcggcgac tcacggtcgc
     1261 tcgccgcgcc gcacgacaag tgaccacaaa ccgcgtgtgc gccggtcctg gccggccagg
     1321 gggatatgcg tgcacctcgg gtccccgccg gcgccgcttc acatgccgac gccctcgcca
     1381 tacctggccg gcatcgcgca cctcgccctg atatcgtcgg cgctcgttcg agggggtccg
     1441 tgtggggcat gaagccgatg tgatcgagga gttacggcgg gacaacgccg cgttgcgcga
     1501 gcgcctcgcc cgggtcgagc cgacggcggc gaagtacgag gtgctggtct cgatcatggc
     1561 cgaggccgtg ctcgtcctcg acagcgacgg gcgctacctg acgatcgagc cgacccaggg
     1621 cttccccccg gaggctcgcg cgcagctgct cggcacctgc ctgcccgacg gcttcggccc
     1681 ggagatcggc ggccagatgg tcgcggcgat ccggcgctcg ctcgccaccg gcgagcccgg
     1741 cacgatcgaa ttccaggtcc ccctgcccga ccgccccctg accgtctgcg cggtgttcaa
     1801 gccgctgacc gccgacaccg tgctgtggta ctcgaacgac atcacggccg agcggctgct
     1861 gcgacaggcc gagcagggcc tgcgcacctt cgaggccgtc gtcgagcgca cccccgacgg
     1921 cgtcgccctg atcaccaccc gcggcgaggt cacgtacacc aaccaggcgt accgcgatct
     1981 cggcggcgcg tttgaccccg gcgacgcact gctcgccgcg cgcctcgcag cccccgagct
     2041 cgtccgcgcc gcgctcgtcg aggcgaccac gcacggacac tggaccgggc ggctcgagct
     2101 cggcgtcgac gccggcgggc gcgtgccctg ccaggtctcg ctcgtgcgct tgccggaggc
     2161 gccgggccgc accgccgagc tcgcggtgat cgcccgcgac ctgcggccgc tgatcgaggc
     2221 cgagggccag cgcctcgcct tgcaagagca ggtgatcgag gcccagcgcg tcgccctgcg
     2281 cgagctctcg accccgctgt tgccgatcgc caccggcgtg gtctgcatgc ccttgatcgg
     2341 cacgatcgac ggcccccgcg cccaggagat gatggagcgc ctgctcgacg gcatcgtccg
     2401 cctgcgcgcc cacacggcga tcctcgacgt caccggcgta cgcgtcgccg acgcccaggt
     2461 cgccgacggg ctgctgcagg tcgcccgcgc cgcccggctg ctcggcgcgc acgtcatcct
     2521 gaccggcatc ggccccgaca tcgcccagac cctcgtcgcg ctgcacagcg agctgctcgg
     2581 catcaccacg ctcggcgacc tgcgcagcgg catcgcccac gccctcgccg gtcgcggggc
     2641 gcccgggtcc gcggcgcggc gcaggccgtg accggatcac agggcgctga ccgcgatcgg
     2701 gcgctgtcag gatagccccg cgatcgtgca ggtccgagga tctgcgccgc agtcactcgc
     2761 ggcccggccg atctcgcggg gtgcgctatc gtcgggggcg gtggccgccg acgacatcga
     2821 cgcccgcgcc ttcgagcccg ccggggcgcg ctggctcgcg cgcatccacg cggcgctcgc
     2881 cggtcccggc cccgcgccga cctacctgct gacgcgcttc gtcctgctgc gcgtgctcgg
     2941 cctcgtgtac ctcgccgcgt tcctgtcggc gctgttgcag ctcgtgccgc tggtcggcgc
     3001 cgacggcctg acgccggccg tcgacgagct cgccgcgcac gccgacgcgg tcggtggcgc
     3061 gtggtccggc ttcctcgagc gcccgaccct gttctggctc gactgctcgg acggcgccct
     3121 gtggggtgtc tccctcgccg gcgtgctgct ctcctgcgtc ttgctgctcg gctgcgagaa
     3181 c
//
LOCUS       DASWNJ010000307         3871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_536479, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000307 DASWNJ010000000
VERSION     DASWNJ010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536479"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="VGB85_05460"
     CDS             <1..860
                     /locus_tag="VGB85_05460"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEY0133501.1"
                     /translation="IELASLRGEIGDRLRSFTAGAGGLELGALVTLAQLAEHAELDAG
                     YAALRQAAASAATPGIRNLATLGGNLLQRPRCWYYRNADLVCLKKGGKACFAVTGENK
                     YHAILGGGPSFIVHPSTLASALLAFDASVEIAGDTERTIPLSELFIGPKTDPKREHSL
                     APGEVLVRIKLPMADKARRSAYSVVKEKQSHDWPLAEAVVSLSLEAGVMKSVRVVLGH
                     VAPTPWRSAEAEAALEGQKPDAAVFARAAAAALAPAKPLKHNAYKIPLAQGLLRDALH
                     RATDIPMPT"
     gene            876..1097
                     /locus_tag="VGB85_05465"
     CDS             876..1097
                     /locus_tag="VGB85_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133502.1"
                     /translation="MPTFLRVHDTTQPVCKHLRTKALHVYGRDTPDAFATSRSSHYHC
                     LRTQFITGPDGGLSVPESCKAARACFEAR"
     gene            1097..1978
                     /locus_tag="VGB85_05470"
     CDS             1097..1978
                     /locus_tag="VGB85_05470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEY0133503.1"
                     /translation="MPHTNTNNSGVFSLAALNRSSGADEGRAALPCYQVDAFTHTRFA
                     GNPAAVVTLDRWLPDPLLQVIAAENSVPETAFLVAKGGDYHLRWFTPEVEVDLCGHAT
                     LAAAFVLCTRVHPGRRSLVFHSQSGDLAVTRDGDEYTLDFPARPPVPADSPAGLVEAM
                     GCTPREVLRARDWLLVLDDAAAVRACRPDMGALAALPDIFGVCVTAPGDIEGVDFVSR
                     FFAPSQGIPEDPVTGSAHCTLAPYWAARLDKHQLRARQLSRRSGELRCTLVGDRVHLS
                     GHAVLTRTGFLLLDDEV"
     gene            2101..3672
                     /locus_tag="VGB85_05475"
     CDS             2101..3672
                     /locus_tag="VGB85_05475"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0133504.1"
                     /translation="MTDPRGPISPPSPEHASLPWRAETLIDDRFQVIRELGHGGMGAV
                     YLVFDRELCRELALKRIHPASVGQQNHEERFRREYRALAAIHHPGVPQVHHSGRAADG
                     AVWFTMEAIRGEPLRSIFDRDRLDPVRALDLAIQLGKILAAAHEAGVIHRDVKPGNIM
                     VEPGDRVRLLDFGICTALPRFLRAAEPRRRTAHVDRWNSGESHFAGSIGYSDPATYDG
                     TPATVRSDIYSLGAIVYEALTGRRLCDPDSLVHRGIDSAELPVELAAFAADLRRATAH
                     NPFERHRTMAEFVQSLEITRGLLARARAAAAPPVRRPTLPLLLGLALGIVLTVVVRDA
                     ASPGHLSSAPAPTPTAPEQSPAQPGPSCASAPCPSPGPSPPVFDPAASPSQSLASSPP
                     VFDPAASSSQSLASSPPTPDPAASPAAPGQPAPRVPASDASGGPSPALTRTRRLLNGR
                     VAAIDGCIRKEGRPLRRLGLALDLDPGGKVRAVRLASGEHSGLSRCVAAALSDMSFPP
                     GSASPLTHTFELTAP"
     gene            3669..>3871
                     /locus_tag="VGB85_05480"
     CDS             3669..>3871
                     /locus_tag="VGB85_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133505.1"
                     /translation="MNLLALTLILSLLPPAADVHNERAMALDEAGRLAEAADELAAAY
                     AAMPDPRADLDGREQVLGSLRGVL"
BASE COUNT          514 a         1648 c         1160 g          549 t
ORIGIN      
        1 tgatcgagct cgcctctcta cgcggcgaga tcggcgatcg cctgcgcagc ttcaccgccg
       61 gtgccggcgg cctcgagctc ggcgccctcg tcaccctcgc gcagctggcc gagcacgccg
      121 agctcgacgc cggctacgca gcgctgcgcc aggccgccgc ctcggccgcg acgccgggca
      181 tccgcaacct cgccacgctc ggcggcaacc tgctgcagcg cccgcgctgc tggtactacc
      241 gcaacgccga tctcgtgtgt ctcaaaaagg gcggcaaggc ctgcttcgcc gtcaccggcg
      301 agaacaagta ccacgcgatc ctcggcggcg gtccgagctt catcgttcat ccttccactc
      361 tggccagcgc actgctcgcc ttcgacgcca gcgtcgagat cgcaggcgac accgagcgca
      421 ccatcccgct ctcggaattg ttcattggtc cgaagaccga tccgaagcgc gagcacagcc
      481 tcgccccggg cgaggtcctc gtacggatca agctgccgat ggccgacaag gcgcggcgct
      541 ctgcatactc cgtcgtcaag gagaagcagt cgcacgactg gccgctcgcc gaggccgtcg
      601 tcagcctgtc cctcgaggca ggtgtgatga agtccgtgcg cgtcgtgctc ggtcacgtcg
      661 cgccgacccc gtggcgcagc gcggaggccg aggccgcgct cgaggggcaa aagcccgacg
      721 ccgccgtgtt cgcccgggcg gccgccgccg cgctcgcgcc ggccaagcca ctcaagcaca
      781 acgcctacaa gatcccgctc gcgcagggcc tgctgcgcga cgccctgcac cgcgcgaccg
      841 acattcccat gccgacctga cgaggagcca ccaccatgcc gaccttcctc cgcgtccacg
      901 acaccaccca gccggtctgc aagcacctgc gcaccaaggc cctgcacgtc tacgggcgcg
      961 acaccccgga cgccttcgcg acctcgcgct cgagccacta tcactgcctg cgcacgcagt
     1021 tcatcaccgg cccggacggc gggctgtcgg tccccgagag ctgcaaggcc gcgcgcgcct
     1081 gcttcgaggc tcgttgatgc cgcacacaaa caccaacaac tccggcgtct tctcgctcgc
     1141 cgccctcaac cgctccagcg gcgcggacga ggggcgcgcc gcgttgccct gttatcaggt
     1201 cgacgcattc acgcacacgc gcttcgccgg caacccggcc gccgtcgtca cgctcgaccg
     1261 ctggctgccc gaccccctgt tgcaggtgat cgccgccgag aactccgtgc ccgagaccgc
     1321 cttcctggtc gccaagggcg gcgactacca cctgcgctgg ttcacccccg aggtcgaggt
     1381 cgacctgtgc ggccacgcca ccctcgccgc cgccttcgtg ctgtgcaccc gcgtccaccc
     1441 cggacgccgg tcgctcgtgt tccacagcca gtccggcgac ctcgccgtca cccgcgacgg
     1501 cgacgagtac accctcgact tcccggcccg cccacctgtc cccgcggaca gccccgccgg
     1561 cctcgtcgag gcgatgggct gcaccccgcg cgaggtcctg cgcgcgcgcg actggttact
     1621 cgtgctcgac gacgccgcgg ccgtccgcgc ctgtcgcccc gacatgggcg cgctcgcggc
     1681 cttgcccgac atcttcggcg tgtgcgtcac ggcccccggc gacatcgagg gcgtcgactt
     1741 cgtgtcccgc ttcttcgccc cctcgcaggg catccccgag gaccccgtca ccggctcggc
     1801 gcactgcacc ctcgcgccct actgggccgc ccgcctcgac aagcaccagc tgcgcgcgcg
     1861 ccagctgagc cgccgcagcg gcgagctgcg ctgcaccctc gtcggcgacc gcgtgcacct
     1921 cagcggccac gccgtgctga cccgcaccgg cttcttgctg ctcgacgacg aggtgtgagc
     1981 ccccggcgct tatagaacga cggagccgta atccagctta cgtcgatttc atcggcgtcg
     2041 ccccggccgc gttgacccgc ttcacggtcg ctgacgacac tgcccgcaca ggaggtcgtc
     2101 atgaccgatc cgcgtgggcc catctctccg ccgtcacccg aacacgcgag cttaccgtgg
     2161 cgcgcggaga ccctgatcga cgatcgcttc caggtcatcc gggagctcgg tcatggcggc
     2221 atgggggccg tctacctcgt cttcgaccgc gagctgtgcc gcgagctcgc gctcaagcgg
     2281 atccaccctg ccagcgtcgg tcagcagaac cacgaggagc gcttccgccg ggaataccgc
     2341 gctctcgcgg cgatccatca cccaggcgtc ccccaggtcc atcacagcgg gcgcgctgcg
     2401 gacggcgccg tctggttcac catggaggcg atccgcggcg agcccctgcg gtccatcttc
     2461 gaccgtgacc gcctcgaccc ggtacgggcc ctggacctgg cgatccagct cggcaagatc
     2521 ctggccgccg cccacgaggc cggggtcatc caccgcgacg tgaagcccgg caacatcatg
     2581 gtcgagcccg gcgaccgcgt gcgcctgctc gacttcggca tctgcaccgc cctcccgcgc
     2641 ttcctgcggg ccgccgagcc ccgccggcgc accgctcatg tcgatcgctg gaacagcggc
     2701 gagagccact tcgccggctc gatcggctac agcgaccccg ccacctacga cggcaccccg
     2761 gccaccgttc gcagcgacat ctactcgctc ggcgcgatcg tctacgaggc gctgaccggc
     2821 cgccgcctgt gcgaccccga cagcctggtc catcgcggca tcgacagcgc cgagttgccg
     2881 gtcgagctcg ccgccttcgc ggccgacctg cgccgggcga ccgcccacaa ccccttcgaa
     2941 cgccaccgga ccatggcgga gttcgtgcaa tccctcgaga tcacgcgcgg cctcctggcc
     3001 cgcgcccgcg ccgcagccgc gccccccgtc cgtcgtccga cgctgcccct gctcctcgga
     3061 ctcgccctcg gcatcgtcct cacggtcgtc gtccgcgacg ccgcatcacc cggccacctg
     3121 tcctctgcgc cagctcccac gccgacagcc ccggagcagt cacccgcaca gccgggtccg
     3181 tcctgcgcgt ccgctccttg cccgtcccca ggtccctcac cgccggtctt cgacccggcc
     3241 gcctcgccgt cgcagtccct ggcttcctcg ccgccggtct tcgacccggc cgcctcgtcg
     3301 tcgcagtccc tggcttcctc gccgcctacc cccgaccccg ccgcctcccc ggccgcgccc
     3361 ggccagcccg ccccgcgcgt gccggcgagc gacgcgagcg gcggcccgag ccccgcgctc
     3421 acccgcaccc gccgcctgct caacggccga gtcgcggcca tcgacggttg catccgcaag
     3481 gaaggtcgcc cgctccgccg cctcggcctc gccctcgacc tcgacccggg cggcaaggtc
     3541 cgcgccgtcc gcctcgcctc cggcgagcac agcggcctgt cgcgctgcgt cgcggctgcc
     3601 ctctcggaca tgtcctttcc accaggctcg gcctcccccc tcacccacac cttcgagctc
     3661 accgccccat gaacctcctg gccctgaccc tcatcctctc gctcttgccg cccgcggccg
     3721 atgtccacaa cgaacgtgcc atggccctcg acgaggccgg ccgactcgcg gaggccgcgg
     3781 acgagctcgc cgccgcctac gccgccatgc cggacccccg cgccgacctc gacggccgcg
     3841 agcaggtgct cggctcgctg cgcggcgtgc t
//
LOCUS       DASWNJ010000308         1521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_393159, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000308 DASWNJ010000000
VERSION     DASWNJ010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393159"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1244)
                     /gene="rsgA"
                     /locus_tag="VGB85_05485"
     CDS             complement(81..1244)
                     /gene="rsgA"
                     /locus_tag="VGB85_05485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEY0133506.1"
                     /translation="MSAKETRRPSPRRRRKPRRINAWLNIERRTQINRTSREPERVKL
                     GLSDPFLEQLGWKPFFSKQVSAEQSQECQPVRVMSVHRGRVTVSGSGIDDSISSSVQS
                     QAGAENRPTVGDWLLIDRNTRDIVRILDRTSLFKRPAPGDDRRTQLIAANVDTLFIVT
                     SCDQDFNVARIERYLVLTREVGVCPVVVLTKSDLASRPELLVDAARALQSGLRVELVD
                     GRDQASAARLADYCGLGQTVALVGSSGVGKSTLVNTLKGSNSIATQDVREDDGKGRHT
                     TTVREMHRVAGGAVGSGWLVDTPGMRELQMSEVAAGVIEVFDDVTAVALECRFTNCTH
                     VDEPGCAVRAAMTTGALDPARVERWRKLTREDAANSGPAAARRSRASKSGNRR"
BASE COUNT          256 a          508 c          461 g          296 t
ORIGIN      
        1 gccggttccg ctcggtgctc cgctctagct actgggtccc gcgattcaga gccgatccgg
       61 cgtgcgcggc cagcaaaccg tcatctcctg ttgccggact tgctggctcg ggagcggcga
      121 gccgccgccg ggccgctgtt cgccgcgtcc tctcgggtta gcttgcgcca ccgttcgaca
      181 cgagcgggat cgagagcgcc tgtcgtcatc gcagcgcgaa cggcgcatcc gggctcgtcg
      241 acatgagtgc agttcgtgaa ccgacactcg agcgcgaccg ccgtgacatc gtcgaagacc
      301 tcgatcacgc ctgcggcaac ttctgacatc tgcagttcgc gcatcccagg cgtatccacg
      361 agccagccgc tgccgaccgc accaccggca acacggtgca tttcgcggac ggtggtggtg
      421 tgccggcctt tgccgtcgtc ctcgcggaca tcttgtgtcg cgatgctgtt tgatcccttc
      481 aacgtgttga cgagggtcga cttacccaca cctgacgatc ccaccaaagc gacggtctgc
      541 ccgaggccgc aatagtctgc gaggcgggcg gcgctcgcct gatcgcggcc atcgaccagc
      601 tccacccgca accccgactg gagcgcacgc gccgcgtcga ccaatagttc aggcctggag
      661 gcgagatcgg acttggtgag cacgaccacc gggcagacgc ccacctcacg tgtcagcacg
      721 aggtagcgct cgatacgcgc aacgttgaag tcctggtcgc aggaggtcac gatgaacagc
      781 gtatcgacgt tcgcggcgat gagctgggtc cggcgatcgt cacccggcgc cggccttttg
      841 aacaggctcg tacgatcgag gatgcgcacg atgtctcggg tgttgcggtc gatcagcaac
      901 cagtcgccaa ccgtgggacg gttttccgct cccgcttgtg attggacgct ggaggaaatg
      961 gagtcgtcta ttcctgaccc cgaaaccgtc acccgtccgc gatgaaccga catgacgcgg
     1021 acaggctggc actcttggct ctgctctgcg gagacctgct tgctgaagaa gggtttccag
     1081 ccgagctgct cgagaaacgg atcggacaaa cccaacttca ctcgctcggg ctcacgactc
     1141 gtccggttga tctgcgtccg gcgttcgatg ttcagccacg cgtttatccg ccttggcttg
     1201 cgccttcttc gcggactcgg ccgccttgtt tctttcgcgc tcacggcgct cgaaatcgta
     1261 attgggtttg cgggctatgt cggtcgctcc ggaactgtgg ttccggctga gcacagcgct
     1321 cgacgtgaaa acccacgccg tcgaggtaca ctgatccctc gctctagaac agtctcacaa
     1381 cgacgcaccg caagaacgtc ggttcgacca gcgttctcct ccgggctgga acgccgtatg
     1441 ccatgtcagc tgccggacgg ctcacatgcg gacgcacagg gcctcttgtc agcttaccgg
     1501 cttcggacgt tcccgcatgc c
//
LOCUS       DASWNJ010000309         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_429972, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000309 DASWNJ010000000
VERSION     DASWNJ010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429972"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="VGB85_05490"
     CDS             complement(<1..862)
                     /locus_tag="VGB85_05490"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0133507.1"
                     /translation="MRVVVTGATGHVGSNLVRALIRRGDQVRVVVRERVASLEGLDVE
                     RVTGDVRDEASLRAAFTDAEIVYHLAAVISISGDRGGAVRAVNVDGTRRVGEAALAAG
                     VRRLVHCSSVHAFNLYQPGAVDPIDESWARVPDSAPHFAYDRSKAAGERALREVIDRG
                     LDAVILHPSGVVGPYDFGPSRMGQVLLQLYHRKLPSLLEGGFDFVDVRDVADGLISAA
                     EKGKSGENYLVTGHFVRVPELARIASSITGVAPPRLSAPIWLARLGVPMVEAYGRVRR
                     KEPLYTRESLA"
     gene            976..>2220
                     /locus_tag="VGB85_05495"
     CDS             976..>2220
                     /locus_tag="VGB85_05495"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0133508.1"
                     /translation="MGEGPQRPAGDIGSDATLDGSMLATVAPPASSAGMSGLSGVSGT
                     SGARGPLLLQRELPADFDRSSLGERERDLVVSRYILLSRLGAGGMGLVYLAYDPSLDR
                     KVAVKLLRPVGDGVEPAAEAHARLHREAQALAKLSHPNVVAVHDVGTYDASALYGTPR
                     GEDSGQGVFLVMEYIEGATLAHWLEERPRSWQEVVAAFLAAGHGLVAAHAVGVIHRDF
                     KPSNVLVRRDGSVHVFDFGLARAAGNTEAVRAEPRADTSSVDLWSSGLSSPLTQFGAF
                     VGTPAYMAPEQRGGEEVDERSDQFSFCVALYEGLYGNRPFSGGNVYLLEMAKVTGQLD
                     PPRRRIRRPKALHRAVVRGLAAEPRDRWPTLPAMLAELDRLLRRRRGRIVAAALAVGL
                     LGAGAGAYAVNRGSVCTDAPAAF"
BASE COUNT          291 a          826 c          815 g          288 t
ORIGIN      
        1 ccgcgagcga ctcgcgggtg tacagcggct ccttgcgacg cacgcggccg taggcctcga
       61 ccatcggcac gccgaggcgg gccagccaga tcggcgcgct caggcgcggc ggggcgacgc
      121 cggtgatgct cgaggcaatg cgggcgagct ccggcacgcg cacgaagtgg ccggtgacga
      181 ggtagttctc gccggacttg cccttctccg cggcggagat caggccatcg gcgacgtcgc
      241 gcacgtcgac gaagtcgaag ccaccctcga gcaggctcgg cagcttgcgg tgatacagct
      301 gcagcagcac ctgacccata cgcgaggggc cgaagtcgta cgggccgacc acgcccgagg
      361 ggtgcaagat gaccgcgtcg aggccccggt cgatcacctc gcgcagggct cgctcgccgg
      421 ccgccttgct gcggtcgtag gcgaagtgcg gcgcgctgtc cggaactctc gcccagctct
      481 cgtcgatcgg gtcgacggcg ccgggctggt agaggttgaa ggcgtgcacc gagctgcagt
      541 ggaccaggcg gcgcaccccg gccgcgagcg cggcctcgcc gacgcggcgg gtgccatcga
      601 cgttgaccgc ccgcacggcg cccccacgat cgccgctgat cgagatcacg gcggcgaggt
      661 gatagacgat ctcggcgtcg gtgaacgcgg cccgcagcga ggcctcgtcg cggacatcac
      721 cggtgacgcg ctcgacatcg aggccctcga gcgaggccac gcgttcgcgg acgaccacgc
      781 ggacctgatc accgcggcgg atcagcgccc gaacgaggtt ggatccgacg tggccggtgg
      841 cacctgtgac gacgacgcgc atgcgggcgc atgctagtac gccggacgcg ggcggtcgac
      901 ggcatcgcgc atcgcggtcg cgcgcgcccc cgcacgcgcc cgcggtgcga tcaatcttgc
      961 tagcctggct cacgcatggg cgagggaccg cagcgccccg cgggcgacat cggcagcgac
     1021 gcgacgctgg acggcagcat gctcgcgacc gtggccccgc cggcgagctc ggcgggcatg
     1081 tccgggctgt ccggcgtgtc cggcacgtcc ggcgctcgcg gcccgctgct gctccagcgc
     1141 gagttgccgg cggacttcga ccgctcgagc ctcggcgagc gcgagcggga tctcgtcgtc
     1201 tcgcgctaca tcctgctctc gcggctcggc gccggcggca tggggctcgt gtacctcgcc
     1261 tacgacccca gcctcgaccg caaggtcgcc gtcaagctgc tgcgcccggt cggcgacggc
     1321 gtcgagccgg ccgccgaggc ccacgcccgc ctgcaccgcg aggcccaggc gctcgccaag
     1381 ctctcgcacc ccaacgtcgt cgccgtccac gacgtcggca cctacgacgc cagcgccctg
     1441 tacggcacgc cgcgcggcga ggactccggc cagggcgtgt ttctggtgat ggagtacatc
     1501 gagggtgcca cgctcgcgca ctggctcgag gagcggccgc ggtcgtggca agaggtcgtc
     1561 gccgcgttcc tcgccgcggg ccacggcctg gtcgccgcgc acgcggtcgg cgtgatccac
     1621 cgcgacttca agccgagcaa cgtgctggtg cgtcgcgacg gcagcgtgca cgtgttcgac
     1681 ttcgggctcg cgcgcgcggc cggcaacacc gaggccgtgc gcgccgagcc gcgcgccgac
     1741 acctcctcgg tcgacctgtg gtcctcgggg ctgtcgagcc cgctgacgca gttcggcgcg
     1801 ttcgtcggca cgcccgcgta catggccccg gagcagcgcg gcggcgagga ggtcgacgag
     1861 cggtccgacc agtttagctt ctgcgtcgcc ctctacgagg ggctgtacgg caaccggccg
     1921 ttctccggcg gcaacgtgta cctgctcgag atggccaagg tcaccggcca gctcgacccg
     1981 ccgcgccggc gcatacgtcg gcccaaggcc ctgcaccgcg cggtcgtccg cggcctcgcg
     2041 gccgagccgc gcgatcgctg gccgacgctg ccggcgatgc tggccgagct cgaccgcctg
     2101 ctgcgacggc gacgcgggcg gatcgtcgcc gcggcgctgg cggtcggcct gctcggcgcc
     2161 ggcgcgggcg cctacgcggt caaccgcggc agcgtgtgca ccgacgcccc ggccgcgttc
//
LOCUS       DASWNJ010000310         2508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_321697, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000310 DASWNJ010000000
VERSION     DASWNJ010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321697"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="VGB85_05500"
     CDS             <1..850
                     /locus_tag="VGB85_05500"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY0133509.1"
                     /translation="ALWREDTGAVHATADRCPHRGASLSLGRVRGGCIECPFHGFRFD
                     GAGTCVEAPCNGAEVPRHLDVRAYVVREAHGFLWLWWGEARESYPEIGWFPELDGPYV
                     HAGLMAQEWPTNWMRSVENQLDWPHLPFVHRTTIGIGAKKAMEVGSVIEGDRLDTWLR
                     NQERPDGTPSLKISLIFPNIWINPFAGARQIGMIAFAPIDAGHTRMYIRTYTRRLPVP
                     GLAALWSRATNVLNRVILRQDTRVVLSQPQAPTTEGSGERLVQADLPIAQFRKQVRRR
                     LPVVRA"
     gene            1851..>2508
                     /locus_tag="VGB85_05505"
     CDS             1851..>2508
                     /locus_tag="VGB85_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133510.1"
                     /translation="MLMLSVACVQTPDRDVPMREYAGAAVPSREVCVQPRRACTGFVD
                     TISGEAALGADDGAAAADALRCADIVKGHATRCPNNRDSRSQAYLCPELVRGPEFPGQ
                     QVFCGPCRSSRAEMLAFGIKALVDAASLDELSGCAGAGRCEGWHRCLEAGTGRMKASD
                     WTADFLGWSLAPERGLLTLDRSGDPGAAVECRPHDAATWPEAVMFFAKLSGLTGNLRC
                     N"
BASE COUNT          377 a          827 c          873 g          431 t
ORIGIN      
        1 cgcgctgtgg cgcgaggaca cgggggcggt gcacgcgacc gccgaccgct gcccgcaccg
       61 tggcgcctcg ttgagcctcg gccgcgtgcg aggcggctgc atcgagtgtc cgttccacgg
      121 gtttcgtttc gacggcgcgg ggacctgcgt cgaggcgccg tgcaacggcg ccgaggtccc
      181 acgccacctc gatgtccggg cgtacgtggt gcgtgaggcc cacggctttc tgtggttgtg
      241 gtggggcgag gcgcgcgaga gctatccaga gatcggttgg ttccccgagc tcgacgggcc
      301 gtacgtgcac gccgggctga tggcacagga gtggccgacg aactggatgc gctcggtcga
      361 gaaccagctc gactggccgc acctgccctt cgtgcatcgc accacgatcg ggatcggtgc
      421 gaagaaagcg atggaggtcg gctcggtgat cgagggcgat cgcctcgaca cctggctgcg
      481 gaaccaggag cgacccgacg gcacgccgag cctcaagatc tcgctgatct tccccaatat
      541 ctggatcaac cccttcgcgg gggcgcggca gatcgggatg atcgcgttcg cgccgatcga
      601 tgccggccat acgcggatgt acattcgtac gtacacccgc cgcttgccag tgcccgggct
      661 cgccgcgctg tggtcccgcg cgaccaacgt gctcaatcgc gtgatcctcc ggcaggacac
      721 ccgtgtggtg ctcagccagc cgcaagcgcc gacgaccgag ggcagcggcg aacggctcgt
      781 gcaggccgac ctcccgatcg cacaattccg caagcaggtc cgtcgccgcc tgccggtggt
      841 ccgggcctga gctgacggct cgacgcagta cgacgcgacc cggaaccccg gcgccggcgc
      901 tgtcgctttc gtctcatgta gacctgcttg cgcacgcggg cgatgacctc gccgggcttc
      961 gtcttgacgt ccgcttcgaa ccagcgaagg tgcttttcac cgctgggcgc ggccgcgcct
     1021 gacggcatcc gatcaggtcg atcggccaga ccctcacggc tctacgcagt acgacgcgga
     1081 cttttccaac cccttgtaag cgctcgtttt tcgccacaag tccgcgtcgt acttcatgaa
     1141 ttcgcccagg gcctgaactc ccgcgagtcc ccgaaactct cgggagtcag cccacatctc
     1201 ggagagctgg tggaggcgct tggagccgcg cagcggcgac aagcgttccg ttccggcgac
     1261 gcgtgtcgct tgggcggtcg cgtgcgggcc cgaccccggc ggagagacgt cgcaggctcg
     1321 ctcggagcga cgcagagcgc ggacatgaac ggcgcgacgt cacagggccc tgggcccgaa
     1381 gcgcaaggga ccgggcacgg gcgaggcatc gaccggcgtg ttcatggttg tgcaaaggtc
     1441 ccgaatttgc gtgcagaatt cgcaccttgc gatgccttcc atctccgtgc cctcgccgag
     1501 ctacgacgct gtccacatgc ttccgcctct tccggtgaac gcagggcgct ccccgtaccc
     1561 ctcgttcgcc agaattccgg cctgaaactt tagcgccttc gacctcgccc acggtgaccc
     1621 tccggccctg gtgccgctgg tggctccccc gttcactgga gcgctcgagg tagtacaaca
     1681 ttcgaccgga ctcatacgcg ctgcgaagtg atgcgcgtgt tggtgacgcg aagcgggtta
     1741 ccattggcgc gtctaggttg aggtggatgg cggacgtggc gaggacagcg cccctttcgc
     1801 gccaggcgcg atgggggtac cgtccgtcag tgctgcgaac tgcgtcaacg atgctcatgt
     1861 tgtcggtggc atgcgtccag acgcccgaca gagatgtgcc aatgcgggag tatgcggggg
     1921 cggcggttcc ctcgcgcgaa gtctgcgtgc agccccgccg cgcctgcacc ggcttcgtgg
     1981 atacgatctc gggggaggcg gcgctcggcg ccgacgacgg agcggccgca gccgacgcct
     2041 tgcgctgcgc cgatatcgtc aagggccacg cgacgcgctg cccgaacaac cgcgactcgc
     2101 ggagtcaggc gtatctttgc ccggaactcg tccgtggacc ggaattcccg gggcagcagg
     2161 tgttttgcgg gccgtgtcgc agttcacgcg ccgagatgct ggccttcggt atcaaggcgc
     2221 tcgttgacgc ggcatccctg gacgagctct cggggtgtgc gggcgctggg cgttgcgagg
     2281 gctggcatcg atgtctggag gcgggtaccg gacgcatgaa ggcgagcgac tggacggccg
     2341 acttcctggg ttggtcgctc gcgccggagc gcggcctatt gaccctcgat cgatcgggcg
     2401 atccgggcgc ggccgtggag tgtcggccgc atgatgcggc gacatggccg gaagcggtga
     2461 tgttcttcgc caagctgagc gggctcaccg ggaacttgcg gtgcaact
//
LOCUS       DASWNJ010000311         1546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_641986, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000311 DASWNJ010000000
VERSION     DASWNJ010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641986"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="VGB85_05510"
     CDS             complement(<1..1040)
                     /locus_tag="VGB85_05510"
                     /inference="COORDINATES: protein motif:HMM:TIGR03915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HEY0133511.1"
                     /translation="MIVRAAIEPTFAAWRARARELLRADIPPAEVLWTDGAQEALFDM
                     SPTTCPKSQADAPRVPAELVALAEVAAHHSDPGRWALLYRVLHRITHGEPELLARVTD
                     PDVVALRARVQAVRRDEHKMHAFVRFRRTEGPDGEHFIAWHRPDHPILPLAAPFFARR
                     FAAMRWSIVTPDASAHWDGHELHFGPGAPRHTAPAPDELEDLFRTYYRNIFNPARLNL
                     RAMRAEMPRKHWATLPEASIIGELTREAPQRTQAMIAAPASAAAAYLPAVRTLPVLRE
                     AASTCRACTICRDATQTVFGEGPASARLMLVGEQPGDEEDRSGRPFSGPAGQLLDRLL
                     AEAGIDRAQVYVT"
     gene            complement(1037..>1546)
                     /locus_tag="VGB85_05515"
     CDS             complement(1037..>1546)
                     /locus_tag="VGB85_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133512.1"
                     /translation="RVYYSAFSPIPHADGRLPGERPPLIREHRLYEADWLVRYYGFSV
                     RELTTEAEPDLSLEHDPKLGWALRNRHLFPVDLQKAPRELLLRVPGLGVRNVERVLQA
                     RRHGPVHLADLARLRVSLRRCMPFLLTADNVRRPAQELDRLALAQRIRPPRQLGLFEA
                     QASAMSGEL"
BASE COUNT          205 a          480 c          665 g          196 t
ORIGIN      
        1 gtgacgtaga cctgagctcg gtcgatgccg gcctcggcga gcaggcgatc gaggagctgg
       61 ccggccggcc cgctgaacgg gcgaccgctg cggtcctcct cgtcgccggg ctgttcgccg
      121 acgagcatca ggcgcgcgct cgccgggccc tcgccgaaga cagtctgcgt ggcgtcgcgg
      181 cagatcgtgc acgcgcggca ggtgctggcg gcctcgcgca gcacgggcaa ggtgcgcacg
      241 gcggggaggt aggcggctgc ggccgaggcg ggggcggcga tcatcgcctg ggtgcgctgc
      301 ggggcctcgc gggtcagctc gccgatgatg ctggcctctg gcagggtggc ccagtgcttg
      361 cgcggcatct cggcgcgcat ggcccggagg ttgaggcgcg cggggttgaa gatgttgcgg
      421 tagtaggtgc gaaacaggtc ctcgagctcg tcgggcgcgg gggcggtgtg ccgcggggcg
      481 ccggggccaa agtgcagctc gtgaccgtcc cagtgcgccg atgcatcggg cgtgacgatc
      541 gaccagcgca tcgcggcgaa gcggcgcgcg aagaacgggg ccgcgagcgg caagatcggg
      601 tggtcggggc ggtgccaggc gatgaagtgc tcgccgtccg gaccctcggt gcggcgaaag
      661 cggacaaagg cgtgcatctt gtgctcgtcg cggcggaccg cctggacccg cgcgcgcagg
      721 gcgacgacat cggggtcggt cacccgcgcg agcagctcgg gctcgccgtg ggtgatgcgg
      781 tggagcacgc ggtagagcag cgcccagcgc cccgggtcgc tgtggtgggc ggcgacctcg
      841 gcgagcgcga cgagctcggc gggcacgcgg ggggcgtcgg cctggctttt tggacatgtc
      901 gtcggggaca tgtcgaagag ggcctcctgg gcgccgtcgg tccacaggac ctcggccggc
      961 gggatgtcag cgcgcagcag ctcgcgggcg cgggcgcgcc aggcggcgaa ggtcggctcg
     1021 atcgcggcgc ggacgatcac agctctcctg acatggcgga ggcctgggcc tcgaacaggc
     1081 cgagctggcg cggcgggcgg atgcgctggg cgagggcgag acggtcgagc tcctgcgccg
     1141 gccggcgcac gttgtcggcg gtcagcaaga agggcatgca gcggcgcagc gagacgcgca
     1201 ggcgggcgag atcggcgagg tgcacggggc cgtggcggcg ggcctgcagc acgcgctcga
     1261 cgttgcggac gccgaggccg ggcacccgca gcagcagctc gcgcggggcc ttttgcaggt
     1321 cgacggggaa cagatggcgg ttgcgcaggg cccagccgag cttggggtcg tgctcgagcg
     1381 agaggtcggg ctcggcctcg gtggtcagct cgcgcaccga gaagccgtag tagcggacca
     1441 gccagtcggc ctcgtagagc cggtgctcgc ggatcagcgg cgggcgctcg ccgggcaggc
     1501 ggccgtcggc gtgggggatc gggctaaagg ccgagtagta cacgcg
//
LOCUS       DASWNJ010000312         2251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_536590, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000312 DASWNJ010000000
VERSION     DASWNJ010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536590"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="VGB85_05520"
     CDS             complement(<1..934)
                     /locus_tag="VGB85_05520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292873.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEY0133513.1"
                     /translation="MTELSIRPDEIRDALQRFVSDYKPETASREEVGIVAETGDGIAR
                     VSGLPSAMANELLEFEDGTLGIALNLDTREIGVVVLGDFDQIEEGQTVRRTGEILSVP
                     VGDAYLGRVVDPLGTPIDGLGEIASSERRALELQAPGVMVRKSVHEPLATGIKAIDAL
                     TPIGRGQRQLIIGDRSTGKTTIAIDTIINQKANWETGDPDRQVRCIYVAIGQKGSTVA
                     AVRGALEEAGSLQYTTIVASPASDSAGFKYLAPYTGSAIAQHWMYAGDASLIVFDDLS
                     KQAEAYRAVSLLLRRPPGREAYPGDVFYLHSRLLE"
     gene            complement(990..1799)
                     /locus_tag="VGB85_05525"
     CDS             complement(990..1799)
                     /locus_tag="VGB85_05525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEY0133514.1"
                     /translation="MHLTGASADALAVLREELDRVLSGGADPARMGDDLFTVSVVLRS
                     EPSLRRVVTDVSLPAEAKQGLVRGLFEGKADRASLELILAAVGRRWRASRDLADSLEH
                     LSEVAVVKSAGSQTARLADELFEVAQVVQHDAALRDALSDPARSIDDKSALVSGLLEG
                     RALPATVTLARQSLAGTYRTVGVALATYQKVAAEVHGEGVATVRVAQPLASDEMTRLG
                     SALSRHYGRAVHLNMVVDPDVVGGIRVEIGDDVIDGTLSARLDSARRQLAG"
     gene            complement(1799..>2251)
                     /locus_tag="VGB85_05530"
     CDS             complement(1799..>2251)
                     /locus_tag="VGB85_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEY0133515.1"
                     /translation="VLDDAGGLGLGRLPHLGDDRSTLLTRLLTDARRLVPGVPELLLQ
                     LGEAKHEAARIREEAREQGSQIVAEMRAQAQSEAGRIVEHGKAQIEAERQQAVTSLRA
                     EVGTLATGLAGRIVGESLDDEARQSRVVERFLAELEASDQGVPRAGAS"
BASE COUNT          312 a          791 c          820 g          328 t
ORIGIN      
        1 gctccagcag gcgggagtgc aggtagaaca cgtcaccggg gtacgcctcg cggcccggcg
       61 ggcggcgcag cagcagcgac acggcgcggt aggcctcggc ctgcttggac aggtcgtcga
      121 acacgatcag ggaggcgtcc ccggcgtaca tccagtgctg ggcgatggcc gagccggtgt
      181 agggcgcgag gtacttgaag ccggcggagt ccgaggcggg cgacgcgacg atggtggtgt
      241 actgcagcga gccggcctcc tccagggcgc cgcggaccgc ggcgacggtc gagcccttct
      301 ggccgatggc gacgtagatg cagcgcacct gccggtccgg gtcgccggtc tcccagttgg
      361 ccttctggtt gatgatcgtg tcgatggcga tcgtggtctt gccggtcgat cggtcaccga
      421 tgatcagctg gcgctggccg cgcccgatcg gcgtcaacgc gtcgatcgcc ttgatgcccg
      481 tggcgagggg ctcgtgcacc gacttgcgca ccatcactcc aggcgcctgc agctcgagcg
      541 cacggcgttc gctgctggcg atctcgccga gcccgtcgat cggagtgccg agggggtcaa
      601 cgacccgacc gagataggcg tcgcccacgg gcacggacag gatctcgccg gttcggcgga
      661 cggtctggcc ctcctcgatc tggtcgaagt cccccagcac gacgacaccg atctcgcggg
      721 tgtcgaggtt cagcgcgatg cccagcgtgc cgtcctcgaa ctcgagcagc tcgttggcca
      781 tcgccgaggg gaggccgctc acgcgggcga tcccgtcccc ggtctcggcc acgatgccga
      841 cctcttcgcg gctcgcggtc tcgggcttgt agtcggacac gaaccgctgg agtgcatccc
      901 ggatctcgtc cggacggatg gaaagctccg tcattgtctc ttccttctct cgtctgctct
      961 agaagtgtgc tggaccggct cgggaccgac tagcccgcca gctggcggcg ggcgctgtcg
     1021 aggcgggcgg acagtgtgcc gtcgatgacg tcgtccccga tctcgacccg gatgcccccg
     1081 acgacgtcgg ggtcgacgac catgttgagg tggaccgcgc ggccgtagtg gcgggacagg
     1141 gcgctgccca gacgcgtcat ctcgtcgctg gcgagcggct gggcgacgcg gaccgtggcg
     1201 acgccttcac cgtgcacctc ggctgcgacc ttctggtacg tcgccagggc gaccccgacc
     1261 gtgcggtagg tgcccgccag ggactgccga gccagcgtca cggtggccgg gagcgccctg
     1321 ccctcgagca gcccggacac cagtgccgac ttgtcgtcga tggagcgggc gggatccgag
     1381 agcgcgtcgc gcagagcggc gtcgtgctgc acgacctgcg cgacctcgaa gagctcgtcg
     1441 gcgagccgcg cggtctgtga gccggccgac ttcaccaccg cgacctcgct gaggtgctcg
     1501 agggagtcgg ccaggtcgcg gctggccctc caccggcgcc cgacggccgc gaggatcagc
     1561 tccagcgagg cgcgatcggc cttgccctcg aagagcccac ggaccagccc ctgcttcgcc
     1621 tctgcgggca gggagacgtc ggtgaccacc cggcgcagcg acggctccga ccggagcacg
     1681 acggagacgg tgaacaggtc gtcacccatc cgggcaggat cggcaccacc cgagagcacg
     1741 cggtcgagct cctcacggag gaccgcgagg gcgtcggccg aggcgccggt caggtgcatc
     1801 agctggctcc agcccgcggg acaccctggt cggaggcctc gagctcggcc aggaagcgct
     1861 cgacgacccg gctctggcgc gcctcgtcgt ccaggctctc cccgacgatg cggccggcga
     1921 ggccggtggc gagcgtcccc acctcggccc gcaaagaggt gaccgcctgc tggcgctcgg
     1981 cctcgatctg ggccttgccg tgctccacga tgcgcccggc ctcggactgc gcctgcgcgc
     2041 gcatctccgc cacgatctgc gacccctgct cgcgggcctc ctcgcggatg cgagcggcct
     2101 cgtgcttggc ctcgccgagc tggagaagca gctcggggac gcccggcacg aggcggcgcg
     2161 catccgtgag gaggcgcgtg agcagggtgc tgcgatcgtc gccgagatgc gggagacggc
     2221 ccaggccgag tccgcccgca tcgtcgagca c
//
LOCUS       DASWNJ010000313         2321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_253648, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000313 DASWNJ010000000
VERSION     DASWNJ010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253648"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1669
                     /locus_tag="VGB85_05535"
     CDS             <1..1669
                     /locus_tag="VGB85_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0133516.1"
                     /translation="ERALVTLLGLPRAAAGLVLGGLVAVDLSDALIVGHQLRWSWPTI
                     AGHLAMWFSLAGPLAVLAAAGVRQAVRADVLASPQATLALRDPPPMTRGLRQAAALAL
                     IAATVAPLVMTELWVQSRARSDAQRTAERRARSLLGAAGPGQEEELGRLIAEIPGAAV
                     HTTTGARYGTPLSPGPGPIGHVADAPADTFTLREADTTVSVAIPRPPPPPIALLGFAA
                     VLCLLAGGSAVGLLCRERERDLQQLRKSLGDDPGPALAPTTPEWSMLARSIDRLVARM
                     HEATIARFVALEKAEETDRLRSQFLANMSHDLRSPLNSILGFSSLLLRGVDGKLDDDQ
                     REMIETIASAGKDLLQQIDAILDMARIEARRIELQAEPVPLAPLISRAVQKARARASE
                     QINYSMETAPGLPTAFVDPRHLVQALENLLLFAGKRLPAGTIAVKLRAGDRDSSLGLQ
                     IQITTPVKPATSQQLDLARRGFHRLPGHRGLGLELPIADALLRIEGCRLDVKEVGGAM
                     LFKIHLPAAESRRKVALIERAREAIPDSSANLPSMPTSPVAAVPAEP"
     gene            1678..>2321
                     /locus_tag="VGB85_05540"
     CDS             1678..>2321
                     /locus_tag="VGB85_05540"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY0133517.1"
                     /translation="MFSRHTAWTSGPGPIDRAFASLAGAEILDLTVSNPTTAGFTHPP
                     DFYRGLCDERSALYEPASLGVASAREAVAAYYAARGRAPDPAHIGVCASSSEAYGFIL
                     GLLCDPGDVVLVPQPGYPLLRILAELAHVELVPYPLAHDGAWHIDLAALAQILAAPPR
                     ARALVVVAPNNPTGNYLSKHELDVLAALLTPRDIALIVDEVFYDYPLTSAPRPSP"
BASE COUNT          301 a         1032 c          674 g          314 t
ORIGIN      
        1 cgagcgcgcc ctcgtcaccc tgctcggcct cccgcgcgcc gccgccggcc tcgtgctcgg
       61 gggcctcgtc gccgtcgacc tcagcgacgc cctcatcgtc ggccaccagc tccgctggtc
      121 gtggccgacg atcgccggcc acctcgcgat gtggttctcg ctcgccggcc cgctcgccgt
      181 gctcgccgcc gccggcgtgc gccaggccgt ccgcgccgac gtgctcgcct ccccgcaggc
      241 caccctcgcc ctgcgcgacc ccccgccgat gacccgcggg ctgcgccagg ccgccgccct
      301 cgcgctgatc gccgccaccg tcgccccgct ggtgatgacc gagctgtggg tccagtcgcg
      361 cgcccgcagc gacgcccagc gcaccgccga acgccgggcc cgcagcctgc tcggcgccgc
      421 cggccccggc caggaggagg agctcggccg cctgatcgcc gagatccccg gcgccgccgt
      481 gcacacaacc accggcgccc gctacggtac tcccctgtcc cccgggccag gtccgatcgg
      541 ccacgtcgcc gacgcccccg ccgacacctt cacgctgcgc gaggccgaca ccaccgtctc
      601 ggtcgcgatc ccccgcccgc cgccgccccc gatcgccctg ctcggcttcg cggccgtgtt
      661 gtgcctgctc gccggcggct ccgccgtcgg gctgctgtgc cgcgagcgcg agcgcgacct
      721 ccagcagctg cgcaagagcc tcggcgacga ccccggcccg gccctcgccc cgaccacccc
      781 cgagtggtcg atgctcgcgc ggtcgatcga ccgactggtc gcgcgcatgc acgaggcgac
      841 gatcgcccgc ttcgtcgcgc tcgagaaggc cgaggagacc gaccgcctgc gcagtcaatt
      901 cctcgccaac atgagccacg acctgcgctc gccgctcaac tcgatcctcg gcttcagctc
      961 cctgctgctg cgcggcgtcg acggcaagct cgacgacgac cagcgcgaga tgatcgagac
     1021 gatcgccagc gccggcaagg acctcctgca gcagatcgac gcgatcctcg acatggcccg
     1081 catcgaggcc cgccggatcg agctccaggc cgagcccgtc ccgctcgccc cgctgatctc
     1141 ccgcgccgtg caaaaggccc gcgcccgcgc gagcgagcag atcaactaca gcatggagac
     1201 cgcccccggc ctgccgaccg ccttcgtcga cccgcggcac ctcgtgcagg cgctcgagaa
     1261 cctgctgctg ttcgccggca agcgcctccc cgccggcacg atcgccgtca agctccgcgc
     1321 cggcgaccgc gactcctcgc tcggcctcca gatccagatc accacccccg tcaagcccgc
     1381 gacctcgcag cagctcgacc tcgcccgccg cggctttcat cgcctgcccg gccaccgcgg
     1441 cctcggcctc gagctgccga tcgccgacgc cctcctgcgc atcgagggct gccgcctcga
     1501 cgtcaaagaa gtcggcggcg ccatgctatt taaaattcac ctgcccgcgg ccgagagccg
     1561 ccgcaaggtc gcgctgatcg agcgcgcccg cgaggcgatc cccgacagct ccgccaactt
     1621 gccgtccatg ccaacgtccc ccgtcgccgc cgtccctgcc gagccataat taaaaaaatg
     1681 ttctcgcgtc acactgcctg gacctccggg cctggcccga tcgatcgcgc tttcgcctcc
     1741 ctcgcgggcg cggagatcct cgacctcacc gtcagcaacc cgaccaccgc cggcttcacc
     1801 cacccgcccg acttctatcg cggcctctgc gacgagcgca gcgccctcta cgagcccgcc
     1861 tcgctcggcg tcgcctccgc gcgcgaggcc gtcgccgcct actacgccgc ccgcggccgc
     1921 gcccccgacc ccgcccacat aggagtgtgt gccagcagca gcgaggccta cggcttcatc
     1981 ctcggcctgc tgtgcgaccc gggcgacgtc gtgctcgtgc cccagcccgg ctacccgctg
     2041 ttgcgtatcc tcgccgagct cgcgcacgtc gagctcgtgc cttacccgct cgcccacgac
     2101 ggcgcctggc acatcgacct cgccgccctc gcgcagatcc tcgccgcccc gccccgcgcg
     2161 cgcgccctcg tcgtcgtcgc ccccaacaac ccgaccggca actatctttc aaagcacgag
     2221 ctcgacgtcc tcgccgccct gctcacccca cgcgacatcg ccttgatcgt cgacgaagtg
     2281 ttctacgatt atccgctcac gtcggccccg cgcccgagcc c
//
LOCUS       DASWNJ010000314         1672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_499954, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000314 DASWNJ010000000
VERSION     DASWNJ010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499954"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VGB85_05545"
     CDS             <1..737
                     /locus_tag="VGB85_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133518.1"
                     /translation="LVGKPANSDAHPILDTGAEKARFMQDAAGFLHSLRWTPAPLLSV
                     LGGLERRPYPPGGIGDERAPHVFLETEQAAALMRLYEDPTTSVAPALSVTAMNLWIDE
                     QRKVEAELPDWDEWIKLTYGIYTEVAPHIDLPATRWWTEVRNTVKEHDAPLELISVID
                     TLDALQMRDGPRLQRGLGDLRARGSRLLSEGMTSIAGMIALELQGADAASRRAFAAEH
                     MSAVTASDEPSAENLAYRALRAHTER"
     gene            994..>1672
                     /locus_tag="VGB85_05550"
     CDS             994..>1672
                     /locus_tag="VGB85_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133519.1"
                     /translation="MPSDPENPSAPAGPETPSGAAEPGLAAHVRFTRGAIGLVTVANV
                     ATVLESLRLLITTGRWQYAVIATAGLGFTAVLLTSAALARRGQVGKAIERVFTSLAVV
                     ILLVPLWFEQVETMLAAAFIFFTLIAAPRLLSLERADRWVYIAVTTGLMLSALELLPL
                     PTRLPAELQYDLPFEMTVLTAMVCFAALAMRDSKRFPVRTKLFLAFLVIALAPLAILS
                     YDASRKVV"
BASE COUNT          219 a          615 c          588 g          250 t
ORIGIN      
        1 tcctcgtcgg caagccggcc aactccgacg ctcacccgat cctcgacacc ggcgcggaga
       61 aggcccgctt catgcaggac gcggccggct tcctgcactc gctgcgctgg acccccgcgc
      121 cgctgctgtc ggtgctcggc ggcctcgagc gccggcccta cccgccgggg ggcatcggcg
      181 acgagcgggc gccgcacgtg ttcctcgaga ccgagcaggc cgccgcgctg atgcgcctgt
      241 acgaggaccc gacgacctcg gtggcgccgg cgctgtcggt gacggcgatg aacctgtgga
      301 tcgacgagca gcgcaaggtc gaggccgagc tgcccgactg ggacgagtgg atcaagctca
      361 cctacggcat ctacaccgag gtcgcgccgc acatcgatct gccggcgacg cgctggtgga
      421 ccgaggtgcg caacaccgtc aaggagcacg acgcaccgct cgagctgatc agcgtgatcg
      481 acacgctcga cgccctgcag atgcgcgacg gcccgcgcct gcaacgcggc ctcggcgacc
      541 tgcgcgcccg cggcagccgc ctgctcagcg agggcatgac ctcgatcgcc gggatgatcg
      601 cgctcgagct gcaaggcgcc gacgcggcct cccgccgggc cttcgccgcc gagcacatga
      661 gcgccgtgac ggccagcgac gagcccagcg ccgagaacct ggcctaccgc gccctgcgag
      721 cccacaccga gcgctaaaaa ctcgcctaca cgcggctcct ggaggcgatc gacggccgcg
      781 ttccgcgagg ctcggcggac gtgccggcat cgatgcctcc cgggcctcga tctgcgcccc
      841 gggcgccggt ctgtagagcc tctgggtggc gattcgccgc gcgatgctcg ttcctgcgcg
      901 cggcggcgcc ggatctgttt tcgagcgcgg gatccggggc tgccgcgagt cgtctgtgaa
      961 cggtgggggg tgtacgctta tcgcctcgcg cgcatgccca gcgacccgga aaatccttcg
     1021 gcgcccgccg ggcccgagac cccgtcgggg gccgcggaac ccgggctggc cgcgcacgtc
     1081 cggttcacgc gcggggcgat cgggctggtg actgtcgcca acgtcgcgac cgtgctcgag
     1141 agcctgcgcc tgctgatcac gaccgggcgc tggcagtacg cggtgatcgc caccgccggc
     1201 ctcgggttca cggccgtgct gctgacctcg gccgcgctcg cgcgccgcgg gcaggtcggc
     1261 aaggcgatcg agcgggtgtt cacctcgctg gcggtcgtca tcttgctggt gccgctgtgg
     1321 ttcgagcagg tcgagacgat gctcgcggcg gcgttcatct tcttcaccct gatcgccgcg
     1381 ccgcggctgc tctcgctcga gcgcgccgat cgctgggtgt acatcgccgt cacgaccggg
     1441 ctgatgctca gcgcgctcga gctgctgccg ctgccgacgc gcctgccggc ggagctgcag
     1501 tacgacctgc cgttcgagat gacggtgctg accgcgatgg tctgcttcgc ggcgctggcg
     1561 atgcgcgact cgaagcgctt cccggtgcgc accaagctgt tcctcgcgtt cctggtgatc
     1621 gccctcgcgc cgctggcgat cctcagctac gacgcgagcc gcaaggtcgt cg
//
LOCUS       DASWNJ010000315         1767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_358415, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000315 DASWNJ010000000
VERSION     DASWNJ010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358415"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VGB85_05555"
     CDS             <1..554
                     /locus_tag="VGB85_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY0133520.1"
                     /translation="QRMHAITDFIAVAQTLIATKATTPALLVAAGESAGGALVAGAVN
                     LRPELFAAAVLRVPFLDAIGTMADPTAPLTTLEYQEWGDPAVRADYDLIKSWCPYTNI
                     AQKHYPPILVESSLADERVAYHEQARYVARLRARGRGGPFVLRTEISAGHGGASSRPK
                     WLRARADELAFILTRLRSLPGAR"
     gene            complement(520..1305)
                     /locus_tag="VGB85_05560"
     CDS             complement(520..1305)
                     /locus_tag="VGB85_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0133521.1"
                     /translation="MTQRNGYPDGAPCWADLTTPDLAGAQRFYGALLGWTFDEGDPAM
                     GHYTTARKGGRSVVGMAPKQEGIDMPTTWSVYLKSGDIDDTARRISAGGGKLLMPPMD
                     IPGQGRMAFAFDPTGAAFGVWQPGTHRGAELFDETGAMCWHEVNTREGAAADAFYRDL
                     FTYEQTQIGDGQQFDYTMWSIDGNPVGGRLQMTADWEGVPPHWITYFAVDDTDASAAR
                     VRELGGKVMHGPFDSPHGKIAVIADPYGAVFSIISRPVATASA"
     gene            1441..>1767
                     /locus_tag="VGB85_05565"
     CDS             1441..>1767
                     /locus_tag="VGB85_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133522.1"
                     /translation="MLRPALLLTTMLLPACAHAPASAPISLRSETVPEDKAQLSFELL
                     YEPKGGRKVELVLKLSVTGLEETNKLVAETYIRHFNVESGSTRWEGFVPPRQPQTIRL
                     LLAIPEG"
BASE COUNT          252 a          648 c          603 g          264 t
ORIGIN      
        1 atcaacgcat gcacgcgatc accgacttca tcgcggtcgc gcagaccctg atcgccacga
       61 aggcgacgac gccggcgctg ctggtcgccg cgggcgagag cgcgggcggg gcgctggtcg
      121 ccggggccgt caacctgcgc ccggagctgt tcgccgccgc ggtgctgcgc gtgcccttcc
      181 tcgacgcgat cggcacgatg gccgacccca ccgcgccgct gacgaccctc gaataccagg
      241 agtggggcga cccggcggtg cgcgccgatt acgatctaat caaatcgtgg tgcccgtata
      301 ccaacatcgc gcaaaagcac tatccgccga tcctcgtcga gtcctcgctc gccgacgagc
      361 gggtcgcgta tcacgagcag gcgcggtacg tcgcgcgcct gcgggcccgc ggtcgcggcg
      421 ggcccttcgt gctgcgcacc gagatctccg cggggcacgg cggcgcgagc tcgcggccaa
      481 agtggctgcg cgcgcgcgcc gacgagctcg cgttcatcct cacgcgcttg cggtcgctac
      541 cgggcgcgag atgatcgaga acaccgcgcc gtaggggtcg gcgatcacgg cgatcttgcc
      601 gtgcggcgag tcgaaggggc cgtgcatcac cttgccgccg agctcgcgca cgcgggccgc
      661 ggaggcgtcg gtgtcgtcga ccgcgaagta ggtgatccag tgcggcggca cgccctccca
      721 atccgccgtc atctgcaggc gcccgccgac cgggttgccg tcgatcgacc acatcgtgta
      781 gtcgaactgc tggccgtcgc cgatctgggt ctgctcgtag gtgaacaggt cgcggtaaaa
      841 ggcgtccgcc gcggcgccct cgcgggtgtt gacctcgtgc cagcacatcg cgcccgtctc
      901 gtcgaacagc tcggcgccgc ggtgcgtgcc cggctgccac acgccgaagg ccgcgccggt
      961 cgggtcgaac gcgaaggcca tgcggccctg cccggggatg tccatcggcg gcatcaacag
     1021 cttgccacca cctgccgaga tccggcgcgc agtgtcgtcg atgtcgcccg acttcaaata
     1081 cacgctccac gtcgtcggca tgtcgatgcc ctcctgcttc ggcgccatgc cgaccaccga
     1141 ccggccgccc ttgcgcgccg tcgtgtaatg ccccatcgcc gggtcgccct cgtcgaaggt
     1201 ccagccgagc aacgcgccgt agaagcgctg cgcgcccgca aggtcggggg tggtcaggtc
     1261 ggcccagcag ggggcgccgt cgggatatcc gttccgctgt gtcatggtgc tccgcgcgat
     1321 ctcgcccgtc agtaccgccg cgacgccgcg cctgtcaacc ccggggcaca ggccggcggc
     1381 gcggccggag tgacggtgtt ccctcgccga taccgcggcg agctgtggta gatgcggtgc
     1441 atgctgcgtc ctgcgctcct cctgacgacc atgctgttgc cggcctgcgc ccatgccccc
     1501 gcgagcgcac cgatctcgct gcgcagcgag accgtgcccg aggacaaggc gcagctgtcc
     1561 ttcgagctgc tgtacgagcc caagggcggc cgcaaggtcg agctcgtcct caagctcagc
     1621 gtcaccggcc tcgaggagac caacaagctg gtcgccgaga cgtacatccg tcacttcaac
     1681 gtcgagtccg gcagcacccg ctgggagggc ttcgtgccgc cgcgccagcc gcagacgatc
     1741 cgcctgctgc tggcgatccc cgagggg
//
LOCUS       DASWNJ010000316         2857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_217463, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000316 DASWNJ010000000
VERSION     DASWNJ010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217463"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VGB85_05570"
     CDS             <1..310
                     /locus_tag="VGB85_05570"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0133523.1"
                     /translation="VPLEITPVMTYGPATGYGRGPKPDFWVSQSQTDSTNGVTPVHVC
                     FAARSRAEVDEFHAAALAAGGRDNGGPGLRPQYHANYYGAFVLDPDGHNVEAAFHGPA
                     "
     gene            complement(317..1522)
                     /locus_tag="VGB85_05575"
     CDS             complement(317..1522)
                     /locus_tag="VGB85_05575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0133524.1"
                     /translation="MTTPIAPIAVLLVEDDRDDYLLTSEFLEEIGADDYTLKWVETYE
                     EGVEALMSGGAELCLLDYRLGARTGIELLREVSERGCTIPIVLLTGQSERSLDIEAMR
                     AGAADYLVKGEFGARQLERSIRYAIERSRSLAALRELNAELQHARNQATNASYAKSTL
                     LASVSHEFRTPLNAILGYSEMVLEELQERGQADLVTDVRRIHAAGSHLLSLVSDILDL
                     SKIEAGKLDLHMRPFDVDKLVVDVSEAIWPLVRHNGNTLVVRGENIGEMTADPTRVRQ
                     VLLNLLGNSCKFTARGQIELTVERRPVREGDFGLMGPTGPTGPAATPGGQCIEFTVKD
                     TGIGMTAAQMQRLFDSFSQVDASVTRRYGGNGLGLAISRRLCRLMDGDIFVDSEFGVG
                     STFCVRLPA"
     gene            complement(1532..1987)
                     /locus_tag="VGB85_05580"
     CDS             complement(1532..1987)
                     /locus_tag="VGB85_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0133525.1"
                     /translation="MVARGRRSPITILIAEDDPEDRMLVREALEESRLANHIRFVEDG
                     EELLDYLLNRGRWSDPEEAPRPGIILLDLNMPRKDGREALREIKAHPDLRRIPVIVLT
                     TSNAEEDILRTYDLGVSSFIVKPVTFDSLVNIMRVLGTYWLEIVDIAGE"
     gene            complement(2119..>2857)
                     /locus_tag="VGB85_05585"
     CDS             complement(2119..>2857)
                     /locus_tag="VGB85_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0133526.1"
                     /translation="VSHAYRDVLGAIVTGSDALHERLDARDAAGEALVREIREAGQRL
                     LGLLTQVLDISDRAVEGPVIELRRVELQPFFHEIAAAIRPLVGHNDNTFELACPDDIG
                     ALETDPTQLGRVLLNLLGNVCKFTRRGRIVLAVARRPARRRDPEAPSPEDRLAEVECI
                     ELAIRPSGLWMTPAQLELLFVSVLPAEVGEPSGARGSGISSSRHLCRTLGGELFVETD
                     GPRRPVLRICLPVHPLADQARTAAHTR"
BASE COUNT          414 a          995 c         1014 g          434 t
ORIGIN      
        1 cgtccccctc gagatcacgc ccgtgatgac ctacgggccc gccaccggct acggccgcgg
       61 ccccaagccc gacttctggg tgtcccagag ccagacggac agcacgaacg gcgtcacccc
      121 ggtgcacgtg tgcttcgcgg cgcgcagccg ggccgaggtc gacgagttcc acgccgcggc
      181 gctcgccgcc ggcggccgcg acaacggcgg gccgggcctg cgcccgcagt atcacgcgaa
      241 ctactacggc gccttcgtgc tcgaccccga cggccacaac gtcgaggcgg cgttccacgg
      301 gcccgcctga cggggcttac gccggcagcc gcacgcagaa cgtcgacccg acgccgaact
      361 cgctgtcgac gaagatgtcg ccgtccatca gccggcacag ccgccggctg atcgccaggc
      421 cgaggccgtt gccgccgtag cggcgcgtca cgctggcgtc gacctgcgag aagctgtcga
      481 acagccgctg catctgcgcg gcggtcatgc cgatgccggt gtccttgacg gtgaattcga
      541 tgcactggcc gccgggggtc gccgcgggcc ccgtcggccc cgtcggcccc atcagcccga
      601 agtcgccctc gcgcaccggc cggcgctcga cggtcagctc gatctgcccg cgcgcggtga
      661 acttgcacga gttgccgagc aggttgagca gcacctggcg cacgcgcgtc gggtcggccg
      721 tcatctcgcc gatgttctcg ccgcgcacga ccagggtgtt gccgttgtgg cgcacgagcg
      781 gccagatcgc ctcgctgacg tcgacgacga gcttgtcgac gtcgaagggg cgcatgtgca
      841 ggtcgagctt gccggcctcg atcttgctga ggtcgaggat gtcgctgacc agcgacagca
      901 ggtgcgagcc ggcggcgtgg atccggcgga cgtcggtcac caggtcggcc tgcccgcgct
      961 cttgcagctc ctcgaggacc atctcgctgt agccgaggat cgcgttgagc ggggtgcgaa
     1021 actcgtggct gacgctggcg agcagcgtgc tcttggcgta gctcgcgttg gtcgcctggt
     1081 tgcgggcgtg ctgcagctcg gcgttgagct cgcgcagcgc cgcgagcgag cggctgcgct
     1141 cgatcgcgta gcggatcgat cgctcgagct ggcgcgcgcc gaactcgccc ttgaccagat
     1201 aatcggcggc gccggcccgc atcgcctcga tgtcgagcga gcgctcggac tgccccgtca
     1261 acagcacgat cgggatcgtg cagccgcgct cgctgacctc gcgcagcagc tcgatgccgg
     1321 tgcgcgcccc gaggcgatag tcgagcaggc acagctcggc gccgccggac atcagcgcct
     1381 cgacgccctc ctcgtaggtc tcgacccatt tgagcgtata gtcgtcggcc ccgatctcct
     1441 cgagaaactc ggaagtcagg agatagtcat ccctgtcgtc ctcgaccagc aacactgcga
     1501 tgggtgcgat gggtgtcgtc atctccgtcc gtcactcgcc cgcgatgtcg acgatctcga
     1561 gccaataggt gccgagcacg cgcatgatgt tcaccagaga gtcgaaggtc accggcttga
     1621 cgatgaacga gctgaccccg aggtcgtagg tgcgaaggat gtcctcctcg gcgttggagg
     1681 tcgtgaggac gatgacgggg atccggcgca ggtcggggtg ggccttgatc tcgcgcagcg
     1741 cctcgcggcc gtccttgcgc ggcatgttga ggtcgagcaa gatgatgccc ggccgcggcg
     1801 cctcctccgg gtcgctccag cgcccgcggt tgagcagata atcgagcagc tcctcgccgt
     1861 cctcgacgaa cctgatgtga ttggcgagcc ggctctcctc gagcgcctcg cgcacgagca
     1921 ttcgatcctc cgggtcgtcc tcggcgatca ggatcgtgat cgggcttcgt cgtccgcgtg
     1981 caaccatgtc gtggtcccgt aatccctcgt gccgtggcag cagcacatcg cacgacacta
     2041 cacgaaaccg gcgcgagaag cccgggcgcg ggggcggcga gagcacggcg tgggccgagg
     2101 tcgtacagcg cgccctggtc agcgcgtgtg ggcggcggtg cgggcctggt ccgcgagcgg
     2161 atgtaccggc aagcagatgc gcagcaccgg gcggcgcggc ccgtcggtct cgacgaacag
     2221 ctcgccgccg agagtgcggc acaggtggcg gctgctggag atcccggatc cgcgcgcgcc
     2281 ggacggctcg ccgacctcgg ccgggagcac gctgacgaat agcagctcga gctgcgccgg
     2341 cgtcatccac agcccgctcg ggcggatcgc cagctcgatg cactcgacct ccgcgagacg
     2401 gtcctcgggt gagggcgctt cagggtcgcg ccggcgcgcc gggcggcgcg cgaccgccag
     2461 gacgatccgc ccgcgccgcg tgaacttgca gacgttcccc agcaggttga gcagcacgcg
     2521 gccgagctgg gtcgggtcgg tctcgagtgc gccgatatcg tcggggcagg cgagctcgaa
     2581 ggtgttgtcg ttgtgaccga cgagcggccg gatcgcggcg gcgatctcgt ggaaaaacgg
     2641 ctgcagctcg acgcggcgca gctcgatcac cgggccctcg accgcgcggt cgctgatgtc
     2701 gagcacctgc gtcagcaggc cgagcaggcg ctgcccggcc tcgcggatct cccgcacgag
     2761 ggcctcgccg gcggcgtcac gcgcgtcgag gcgttcgtgc agcgcgtcgc tgccggtgac
     2821 gatcgccccg agcacgtcgc gataggcgtg gctcact
//
LOCUS       DASWNJ010000317        10229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_146985, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000317 DASWNJ010000000
VERSION     DASWNJ010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 10229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10229
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146985"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VGB85_05590"
     CDS             complement(<1..422)
                     /locus_tag="VGB85_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0133527.1"
                     /translation="MSRAGDLLEGRYELEAQLGAGGTARVFRATDLVTKAIVAIKLME
                     PGREQDPEALAYFRQEGRLAARIRDPHLVPALHFGVDEGRHFIVYDYLPGSAPLSALA
                     DLGRVAPRRVCEMALQVLYALDTLHRASVVHGDVSLNN"
     gene            complement(432..719)
                     /locus_tag="VGB85_05595"
     CDS             complement(432..719)
                     /locus_tag="VGB85_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0133528.1"
                     /translation="MLASEVSEAAGVSFVTLRDWVRRGILPPPVESLRGRGNIAKYPP
                     SAVPRADLARRLRDDGWSLDGIAEHIKSKPWDGPAPAKGPTESDPEEADED"
     gene            1775..2242
                     /locus_tag="VGB85_05600"
     CDS             1775..2242
                     /locus_tag="VGB85_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133529.1"
                     /translation="MAPKIAFSEPLPFFHHAASERVLYVTHGPSPSPLVFSIGLKDVA
                     RHELICRGPVLTNATHPFGTSTGVSSLVEGSVRRGLAEAEIFEDPGFLQDTLELWRDF
                     CDAVAERLQNDEDLELALAMTWVLENAEDVDLNDLAANSAVGPAALTKPHGAA"
     gene            2239..2475
                     /locus_tag="VGB85_05605"
     CDS             2239..2475
                     /locus_tag="VGB85_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133530.1"
                     /translation="MKPGKKPVTLPDLTLAGAERHLCVEALARTGSLIEAAELLGTNR
                     HALRRLIAKHEIEWWPYAQQQTRWKAPRQQARAH"
     gene            2505..3398
                     /locus_tag="VGB85_05610"
     CDS             2505..3398
                     /locus_tag="VGB85_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133531.1"
                     /translation="MRTSIASALVLALSLPPGAAHAGDWSAEQKAAMQQIITLRNGGA
                     LDEAVTLAQQEFTRADASKGFRRAVAREGKAAAIKVFERDRASPRAAVAVAALCVAVD
                     LMRAYQADLMESENDRLKIPAEVMRLEALATTAAAPCAPSSAPAAPPPQPPLVAVEKP
                     APALGVSTSPPPLASPPVDRADRRRFRAGLGTLIPGLLLFAPMAGLLAYRGDGERELA
                     AIRVDTKNGTPTPKQDQDAAALGQRYTATTAGTVALGVTGAALVVTGAALLATGARQH
                     RMAVAPWGGHGVGGLVLQGRF"
     gene            3446..5152
                     /locus_tag="VGB85_05615"
     CDS             3446..5152
                     /locus_tag="VGB85_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133532.1"
                     /translation="MRRLSFVVLGVLVSGCTSPQYQAYLEEHADAAGASSGASSGDME
                     STITGDMESASTGAIGSESAGASATDTVDTADTDPTGTGDATDTGTDTADSPPVGEPE
                     KPFIVSVELPENVYSAGPVPLAVQTDHTSSVSVTVDGADAGELIAAGGGLFTGELPVR
                     GAIDNGSHEIEVIATQGKYEDRRADAYNVKTPAPGTEAWSKTGPTGSRTNRVATTPAG
                     HLIEVGQTLIDGVPRPTIRLRSGVTGAELWPEGTIVLDTREGAVVDVAVLADGRRWVA
                     MNVREGKMDPRPRLALLDADGHATGIEAEGATGRVVRAIAADADGGCFAVGLAVVLGD
                     WDAAYWRITAAGEQTLGDAYDYRPTLKDPPHTFAEFANDVLIDGDVVWVIGASMGKHD
                     AKFDPLTRGMIARLDLHTGDVIAPTIIAPADGDWKESVFFGAALHPEGVVVTGYGRDP
                     IASLYRIETSGYTAAGERPWYQRETSNSGLAYGSDVALDSQGRALVTGAVTQNGKLRG
                     YVFGRAVDVKDGSILLEHWFPGVGPSEGLGIARDAFDRIYPVGYVTSSGTTQARLTRI
                     HG"
     gene            complement(5184..6290)
                     /locus_tag="VGB85_05620"
     CDS             complement(5184..6290)
                     /locus_tag="VGB85_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133533.1"
                     /translation="MTRAPILAALLLAACGDATPPGFSTGPAITSVSATTGGSSTGGD
                     TSTGPADDSAGSSAGASTSAGTLDVGTVMDFGPVQPLGCKGKVDLLFLIARTGTMVTE
                     QAQLLASFSGFVDTIEEKLEGFDVHIMAANPDGTWPAWNVCEYGANQCNDYWPNCGPN
                     AEDYVCYEYAQLATPCDEKLGAGLIFNTGGYAANRLCDLYGGNRYIVSGEPKLDDALE
                     CIAKVGMSGGSGPIGEALVAALSYDLNKPEGCNAGFLREDALLVVTIISDTNDTDSVT
                     WPYQWYDKIIAAKKDPSAVVMLGVVPQHPVEGEEEKPGCTYHALDEPNGKIRDLIDMF
                     PYKAYGDTCAPSYAPFFDNAADMIGEACGSFIPQ"
     gene            6531..6998
                     /locus_tag="VGB85_05625"
     CDS             6531..6998
                     /locus_tag="VGB85_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133534.1"
                     /translation="MNPWIPVIYGATPKATSLRLVGRNGDEDRGDPGDSNDRNPVTAC
                     RRVSRVAEILKALRHRKYSGPRARLRCARAGRLRLRPTLDATLEFGFGYRASPLAITE
                     VVTLGAVAVNDIFSATRAVTTTSGPGLFAGIGLVLAARCPSPEFKIRPVGGRA"
     gene            7410..8939
                     /locus_tag="VGB85_05630"
     CDS             7410..8939
                     /locus_tag="VGB85_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEY0133535.1"
                     /translation="MANPEALAAELAEHYARILGQQADRQVSDVVRTCLRRLPSRLPI
                     QGEDLRAVEELVERLIRLGEVGARDPIGSPREIDDYIARRLGEPASGARRVYRALRAM
                     LGHTVLVERRSRQIWVLCLSGLIDPSSRLHRTLLAETPALYSLPASDTDSVATSDAPH
                     GVATTDEPVTPSQPPEPLADPSQQLRAELARLHDELAAERLARVEGDKRREAADAARD
                     EEKRARDEADLRAQVATTQLTALEQEVGALRRALEQERQARAQADLRGQAGAAERTAL
                     AQKIGSLRRALDEERLARGEADKRGQVAEAELAAMRDAQAADAATMRRLIECDQALGA
                     AAFLMARMHGATVEDAIQAVHRVMPAAAAVLLASRDQSLNADRMGGAPADPAADSAPQ
                     SQVDPRPQGALQRTSSASTVGPNAPALAVPLPSPLPSASASSDRSAVTQAPDIEPAEL
                     SGRLPTQRPRTSQAQVFPTPGMALDQPQHHVKPSKTGRNDPCPCGSGTKYKRCCGRAA
                     P"
     gene            9538..9771
                     /locus_tag="VGB85_05635"
     CDS             9538..9771
                     /locus_tag="VGB85_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133536.1"
                     /translation="MEMLRFGHVGGEHRLAGAVRLSRQFDARAKLGDRERTACGVLDV
                     DTAAAEIARRVADDTLESVFSRSTTGQSLSATG"
BASE COUNT         1636 a         3466 c         3525 g         1602 t
ORIGIN      
        1 cagttgttga gcgacacgtc gccatgcacg acgctggcgc ggtgcagcgt gtcgagggca
       61 tagagcacct gcagggccat ctcgcagacg cggcggggcg ccacgcgccc gagatcagcc
      121 agagcactga gcggcgcgga gccgggcagg tagtcgtaca cgatgaagtg acggccctca
      181 tccacgccga aatgaagcgc ggggacaaga tgcgggtcgc ggatgcgtgc ggcgagtctg
      241 ccctcctggc ggaagtaggc gagggcttcg gggtcttgct cccggccggg ttccatgagc
      301 ttgatcgcca cgatcgcctt ggtcacaagg tcggtcgccc ggaagacgcg ggccgttccg
      361 ccggccccga gttgggcctc gagttcgtag cggccttcca gaagatcgcc ggcgcgggac
      421 atggctcaag cctaatcctc gtcggcctcc tcggggtcac tttcggttgg gcccttcgcg
      481 ggcgccggac catcccacgg cttggacttg atgtgctccg cgatgccgtc gagcgaccag
      541 ccgtcatccc ggagccggcg cgccaggtcg gcgcgaggga ccgcggaggg tgggtacttc
      601 gcgatgttgc cccgcccgcg aagcgactcg accggcgggg gtaaaatccc ccggcgaacc
      661 cagtcccgca gggttacgaa gctgacgccc gcggcctcgg agacctcgct ggccagcacg
      721 aacgtccgac gtggttttgg aagccgggcc ggcaccggcg gcactctggc ggaaagtacc
      781 cggcgccgca tcatctttca tcttgcgatt tttaatgatt ttcttatgat ttcgcggtgc
      841 tggaagcgtc gtcgccccac gtctgggtct tgatgtgctc cgcgatggcg tcgagcgacc
      901 atccatcgtc gcggagtcgg cgtgccaggt cggcgcgagg gacggcggac agcgggtatt
      961 tagcgatgtt gccccgtccg cgaagggact cgaccggcgg cggcaaaatc ccccggctga
     1021 cccagtttcg cagggtaacg aaactaacgc ccgccgcctc tgcaacctcg gtcgcgagcg
     1081 cgaacgtccg gcgcggtttt ggcatcctac gcggcaaggc ggcagtctag gccgaaaatg
     1141 cccacattcg caccataaaa tctgtcttgc gattttcttg tattttctta tgattttttc
     1201 ggagggtaaa ctgccatgtc ccaacgaagt agaatcgaag tcgccgcgtt cgacccggaa
     1261 acagcccgct acgttgtaat gggtaaaaat aagtcctacc cggacccgcg aagcgcccag
     1321 gccgcgggtt gcatcgtgtt gcgtggtccg ctcaccgtgg ccggcgtggt ctcgctgtcc
     1381 atggaccggg ttgtagaggt cccgagggcc ccgctggtga tctccaacgt gcagggcaca
     1441 gagtaccggc cgccgcgccc aaagccgtcc caggagccgc cacgcgcccg tcagagccgg
     1501 aggtcgcgat gaacggcgct cgcccagcac ggatgatccc ggcctccgcg ctgacgcccg
     1561 gcctggccct gatcatcctc cgtctcccgg gtcgaacgat ccgaagctgt gccgtcatgg
     1621 tccaggcaca gccgcacggc gtcgtgcaag tcgcgttcgc ctcgggcgac gtgctcacgc
     1681 tctcggcccg gacgatggtc ggcagcatcg atcgacccgc ccggtcgcga cctccgcctc
     1741 gaacccggag cgcctcgtga aaggcaggat ctccatggca cccaagatcg ccttcagcga
     1801 gccgctgcca ttcttccacc acgccgcgag tgaacgggtc ctgtacgtca cgcacggccc
     1861 ttccccgtcc cccctcgtct tctcgatcgg cctcaaggat gttgcgaggc acgagctgat
     1921 ctgtcgcgga cccgtgctga cgaacgcgac gcaccccttc ggcacgtcga ccggggtctc
     1981 gtcgctcgtt gaaggatccg tccggcgcgg cctggcggaa gccgagatct tcgaggatcc
     2041 ggggttcctg caagacaccc tcgagctgtg gcgggacttc tgtgacgccg tggcagagcg
     2101 cctgcaaaat gacgaggatc tcgagctggc gctggcgatg acgtgggtcc ttgagaatgc
     2161 cgaggacgtc gatctgaatg acttggccgc caattcagcc gtgggcccgg ccgcactgac
     2221 gaaaccccac ggggccgcat gaagccgggc aaaaagccgg tcaccctgcc ggacctgacc
     2281 ctcgcgggcg ccgagcgtca cctgtgtgta gaggccctgg cgcgcacggg ctcgctgatc
     2341 gaggccgccg aactgctcgg caccaaccgc cacgccttgc ggcgcttgat cgccaagcac
     2401 gagatcgagt ggtggcccta cgcgcagcaa cagacccgat ggaaggcgcc tcgccagcag
     2461 gcacgcgccc actaacccag caggccactg tgttacggtt tgtcatgcgc acgagtatcg
     2521 cgtctgctct cgtcctggcc ctgtccttgc cgccgggggc cgcccacgcg ggcgactggt
     2581 cggccgagca gaaggccgcg atgcagcaga tcatcaccct gcgtaacggc ggcgccctgg
     2641 acgaagcggt cacgctcgca cagcaggagt tcacccgcgc cgatgcgagc aagggcttcc
     2701 ggcgagccgt cgcacgtgag gggaaggcgg cggcgatcaa ggtgttcgag cgagaccggg
     2761 ccagtccgcg ggcggcggtg gcggtggctg ccctctgcgt ggccgtcgat ctcatgcggg
     2821 cctaccaggc cgatctgatg gagtcagaga acgaccggct caagatcccg gcggaggtga
     2881 tgcgactcga ggcgctggcg acgacggcgg cggcaccgtg cgcgccatca tcggcaccgg
     2941 ctgcgccacc accacagcct cccctcgtcg cggtcgaaaa gccggccccg gcactcgggg
     3001 tgtccacctc gccgccgccg cttgcgtcgc cgccggtcga ccgcgccgat cgccgccgct
     3061 tccgggccgg gctggggacg cttatccccg gcctcctgtt gttcgcaccg atggccgggc
     3121 ttctggccta ccgcggcgat ggggagcgcg agctcgcggc gatccgcgtc gacaccaaga
     3181 acggtacccc cacaccgaag caggaccagg acgcggccgc gctgggccaa cgctacacgg
     3241 ctacgaccgc ggggaccgtc gcgctcgggg tgaccggcgc cgcgctcgtc gtgaccggcg
     3301 cggccctact ggcaaccggc gcgcggcagc accgtatggc ggtcgccccg tggggtggtc
     3361 atggcgtcgg cggcctcgtc cttcagggga gattctgacg atgttcgatt tcgccatttc
     3421 gccacttccg ctacagtgcc ctcgtatgcg ccgcctctcc tttgtcgtcc tgggcgtcct
     3481 cgtctcgggg tgtacctcgc cgcagtacca ggcttacttg gaggagcacg cggatgcggc
     3541 aggcgcctcg agcggcgcct cgagcggcga catggagtcg acgatcaccg gcgacatgga
     3601 gtccgcgagc acgggtgcga tcggcagcga gagcgcaggc gcgtctgcga ccgacacggt
     3661 cgacacggcg gacacggacc cgaccggcac cggcgacgcg accgacaccg ggaccgatac
     3721 ggccgacagc ccgccggtcg gcgagcccga gaagccgttc atcgtctcgg tggagctgcc
     3781 agagaacgtc tactcggccg ggcctgtgcc gctcgcggtt cagaccgatc acacatcgtc
     3841 ggtgagcgtc acagtcgatg gcgcggacgc gggcgagcta atcgcggcgg ggggcgggct
     3901 gttcacgggc gagctgcctg tccgcggcgc gatcgacaac ggctcgcacg agatcgaggt
     3961 catcgccacg cagggcaagt acgaggaccg ccgcgcggac gcctacaacg tcaagacgcc
     4021 cgcgcccggc accgaggcgt ggtcgaagac gggccccacc ggcagccgca cgaaccgcgt
     4081 ggcgaccacg cccgcggggc acttgatcga ggtcggccag accttgatcg acggtgtgcc
     4141 gcggccgacg atccgcctga gatcgggtgt gaccggcgcg gagctgtggc ccgaggggac
     4201 gatcgtcctc gacacgcgcg agggcgccgt ggtcgacgtc gcggtcctcg ccgacgggcg
     4261 caggtgggtg gcgatgaacg tgcgcgaggg aaagatggac ccgcgcccgc ggctggcgtt
     4321 gctcgacgcg gacggccacg cgaccgggat cgaggccgag ggcgccaccg gccgcgtggt
     4381 ccgtgccatc gcggccgacg cggacggcgg ctgcttcgcc gtgggcctcg ccgtcgtcct
     4441 cggcgattgg gatgccgcgt actggcggat cacggccgcg ggcgagcaga cgctcggtga
     4501 tgcgtacgac taccggccaa cgctcaaaga tccgccgcac acattcgcgg agttcgcgaa
     4561 cgacgtgctg atcgacggcg acgtcgtgtg ggtcatcggc gccagcatgg ggaaacacga
     4621 tgcgaagttc gatccgctca cgcgcgggat gatcgcgcgg ctggacctcc acactggcga
     4681 cgtaattgcg cccacgatca tcgccccggc ggacggtgac tggaaagaga gcgtgttctt
     4741 cggcgcggct ctccaccccg agggcgtggt cgtgaccggc tacggccgcg atcctatcgc
     4801 ctcgctgtac cggatcgaga cctcgggcta caccgccgcg ggtgaacgcc cgtggtacca
     4861 gcgggagacc tcgaacagcg ggctggccta cggcagtgac gtcgcgctcg acagccaagg
     4921 acgcgccctc gtcacggggg ccgtcacgca gaacggcaag ctacgcggct acgtgttcgg
     4981 ccgggcggtc gacgtgaagg acgggtccat tctgctcgag cactggttcc cgggcgtggg
     5041 cccctccgag ggactcggca tcgcccgcga cgccttcgat cgcatctacc cggtcggcta
     5101 cgtcacctcg agcgggacga cgcaagcccg gctcacacgg atccacgggt aggcgtgcgg
     5161 cgcatcgcgc cgtgcagggc gagtcactgc gggatgaagc tcccgcacgc ctcgccgatc
     5221 atgtccgccg cgttgtcaaa gaatggcgcg tagcttgggg cacaggtgtc gccgtaggct
     5281 ttgtaaggga acatgtcgat caggtcgcgg atcttgccgt tcggctcgtc caaggcgtgg
     5341 taggtgcagc ccggcttttc ctcctcgccc tctacagggt gctgtgggac gacgccgagc
     5401 atgacgacgg cgctcggatc ctttttggcc gcgatgatct tgtcgtacca ctgatacggc
     5461 caggtgacgg agtcggtgtc gttcgtgtcg ctgatgatcg tgacgacgag cagcgcgtcc
     5521 tcccgcaaga accccgcgtt gcagccctcc ggcttgttaa ggtcgtagga gagcgcggcg
     5581 accagcgcct cgccgatcgg cccggaacct ccagacattc caaccttggc gatgcactcg
     5641 agcgcgtcgt ccagcttcgg ctcgccgctg actatgtagc ggttgcctcc gtacaggtcg
     5701 cacagtctgt tcgccgcgta gccgcctgtg ttgaagatga gtccggcccc gagtttctcg
     5761 tcgcacgggg tcgcaagctg cgcgtactcg tagcacacgt agtcctcggc gttcgggccg
     5821 cagttcggcc agtagtcgtt acactggttg gcgccatact cacagacgtt ccatgcgggc
     5881 caagtcccgt ccgggttggc ggccatgatg tggacgtcga agccctcgag cttctcctcg
     5941 atcgtgtcga cgaagccgga gaagctggcg aggagctgag cctgctcggt caccatcgtg
     6001 ccggtgcgcg cgatcaagaa cagcaggtcg accttgccct tgcagcccag cggctggaca
     6061 gggccgaagt ccatcacggt cccgacatcc agcgtgcccg cactcgtgct cgcgcccgcg
     6121 gagctgccgg ccgagtcgtc cgccggcccg gtggacgtgt cgccgccggt ggagctgccg
     6181 cccgtcgtcg cggacacgct cgtaatcgcg ggcccggtcg agaacccggg cggggtcgcg
     6241 tccccgcatg cggcgaggag cagggcggcg aggatgggcg cgcgcgtcac accgcaaggg
     6301 tagacctcgc cacgggcgat gtcacgccgg gcgcccgccg cgaccgagac ctgccacgga
     6361 gtaaatgcct gcctgcccgg cgccgcaggc ggggtcgcgc gggcccgtac gcctggcgca
     6421 cgtcgtgggc caggttaccg gcgccgatcg acaccgccgc gagcgtcgcg tcgcgctgac
     6481 gggtggccga ggccgaaccg cggcttgtag ttgccaacgt tgcgatcttt atgaacccat
     6541 ggatcccggt catctatggc gcgacgccga aggcgacgtc gctgcgtctg gtcggcagga
     6601 acggggacga ggatcgcgga gatcctggag attcgaacga tcggaaccca gtcaccgcgt
     6661 gccgccgagt ttcaagggtc gcggaaatat taaaggcgct gcgccaccga aaatactcgg
     6721 gaccgcgagc gaggttgcgg tgcgcacggg cgggccgcct gcgcctgcgc cccacccttg
     6781 acgccacgct cgagttcggc ttcggctacc gcgccagtcc gctggcgatc accgaggtcg
     6841 tcacgctcgg cgccgtcgcg gtcaacgaca ttttctcggc gacccgagcc gtgaccacga
     6901 ccagcggccc cggcctgttc gccggcatcg ggctggtcct cgctgcgcgc tgcccctcac
     6961 cggagttcaa aatccgaccg gtcggcgggc gggcttgatc gcgccccctg cgatgatcga
     7021 gttatgcgcg agccgccgcc gagttggcta ccggccaggc acgactgcac gtcgagtgcg
     7081 tgcagctcgt gctcgtctgc atggcgagag agcacggact tgtgctcatg ttcgtgtcga
     7141 tgtggtgcag agttcccctc aggatcaacc gactgccgac caagccgcgc tggagcgcgc
     7201 ggagagtgtg cgtcgagtcg gcgccaagta ggtcacgata cgggcacgat atgggcccga
     7261 tacggacccg atgagtgcgg tggaagctcc cggtatcggc gggatgttgg cgggcgcgag
     7321 attggctgtc cacccctacg gggccctcgg atatcctctc tgatggggtg tcggggatgg
     7381 agaggcggac ggcggacagg cgtgctcgcg tggcgaaccc agaggcgtta gctgccgagc
     7441 tggcggagca ctacgctcgg atcctcgggc agcaggccga ccgacaggtc tccgacgtcg
     7501 ttcgcacgtg cctgcgccgg ctcccgtcgc gcctcccgat ccagggtgag gaccttcgtg
     7561 ccgtcgagga gctggtcgag cggttgatcc ggctcggaga ggtcggcgcc cgcgatccca
     7621 tcggttcacc ccgggagatc gacgactaca tcgcgcgccg actcggcgag ccggccagcg
     7681 gagcgcggcg cgtgtaccgt gcgctccgcg cgatgctcgg ccacacggtc ctcgtcgaac
     7741 ggcgcagccg tcagatttgg gtgctgtgcc tgtccggcct catagacccg tcctcgagac
     7801 tccaccggac gctgctggcc gagacaccgg cgctctactc gctgccagca tccgacaccg
     7861 actcggtcgc cacgtccgat gccccacacg gcgtggccac aaccgatgag cccgtcaccc
     7921 cgtcgcagcc cccggagccg cttgcggatc cgtcgcagca actccgcgcc gaactcgccc
     7981 ggctccatga cgagcttgcc gccgagcggc tggcgcgagt cgagggggat aagcgccgag
     8041 aggccgccga cgcggctcgc gacgaggaaa agcgcgcacg cgacgaggcg gacctgcggg
     8101 cccaggtcgc cacaacccag ctcaccgcgc tcgagcagga ggtcggcgcg ttgcgccgcg
     8161 ccctcgaaca agaacgccaa gcgcgcgcgc aggcggacct gcgtggccaa gccggcgcag
     8221 ccgagcgcac cgcgctcgcg cagaagattg gctcgctgcg ccgcgcactc gatgaggaac
     8281 gtctggcgcg tggcgaggcg gacaagcgcg gccaggtcgc cgaggccgag ctcgccgcca
     8341 tgcgtgatgc gcaggcggca gacgcagcca cgatgcggag gctgatcgaa tgcgatcagg
     8401 cgctcggggc ggctgccttc ctcatggcgc gcatgcacgg tgccaccgtc gaggacgcga
     8461 tccaggccgt ccaccgggtc atgccggccg cagccgccgt actcttggcg tcgagggacc
     8521 agtcgctgaa cgctgaccgc atgggcggcg cgccagcgga ccccgctgcg gacagcgccc
     8581 cgcagtcaca ggttgacccg cgcccccagg gcgcactcca gcgcacgagc agcgcgtcga
     8641 cggtcggccc aaatgcacct gcgctcgctg tgccgctacc atcgccgctg ccgtcagcct
     8701 cggcgtcgtc cgaccgctcc gccgtgaccc aggcgccgga catagagccc gcggaattat
     8761 cgggcaggct cccgacgcag cggccacgca cgagccaggc gcaggtcttc cctacgcctg
     8821 gcatggcgct cgaccagccc cagcaccacg tgaagccgag caagaccggg cgaaacgatc
     8881 cgtgcccgtg cggctcaggc acgaaataca agcgatgctg cggtcgagcc gcgccgtagg
     8941 tgcgtggcgc tgtgcggacg ccggcatgat ttcggcctcg gtactgcgaa gctcgcgggc
     9001 cagggacatt tgggccggaa gtaattctgc gaggaaatgg agcctgcctg cgcgacagcg
     9061 tgaacatgtc gggtggcggc caagtcgagg tcgaggccaa gcaggcgctg acagccagac
     9121 acagggtgcg cggcgctgtg caaggggccg tggacggagg tcggctcttg ggacttctga
     9181 cagaaaccca gaaggccagc cggtgctttg tcgtggcgcg cgcctttgtc agaaaaagtc
     9241 tcagcgccaa gcctgtcctt cagcacgcgc agcaactccg cggctgaggc gttggcctcc
     9301 gttgatccgg tccgtttttc gccaccagaa tttatcccgg caattccgag accatgccga
     9361 gcagcgaagc tcgctgctcc gaggaataga aggtaggcgc cgtcccgcaa tacaaccgac
     9421 cgctagggcg cctggcgccg gcccgacttc tggattttct atcagaacat ccaacaactc
     9481 ggtcgagctc gcgcggtgcc cggcgcggtc cccctgttcc cggtccacga cacgagcatg
     9541 gagatgctgc ggttcgggca cgttggcggc gagcaccggc tcgcgggagc agttcgcctc
     9601 tcccggcaat tcgacgcacg cgcaaaactc ggcgaccgcg agcgcacggc gtgcggcgtc
     9661 ctggatgtcg acactgcggc tgcagaaatc gccaggcgag tagcggacga cacgctcgag
     9721 tcggtatttt cgcggagcac gacgggacag tcgctgtcag ccaccggctg accttctggg
     9781 ttttctgtca gaactctcaa gaacctggcc attcacgcgc ggggcgcggg ccgggtctgc
     9841 ccgttcatcc ggccgctgat gtggcgtgca gagctgattt tccccgcccc gatttttgcg
     9901 tgtggcgagc gagaccaagt atcgatgtct ctcccacgtg gtctcaaaaa tggctgcggt
     9961 cgcgttcggt ggagcggaga cagacaccgt cgcgtggcgc agtttgccct cgacctctcg
    10021 agtgcacggc cgcgctcgcc gctcggtcgc gttgtcgacc acgaaggcgc atgacctgtc
    10081 cgtaaggaca tgtcgcgcgg gctcaatccg cgcggcgcat ttgcgccgca ttcttcggcg
    10141 ttctgtcaga ggccgcagaa gtgccttgca acgaccggca ggctgaggtt cgatggaggg
    10201 atgaagtggc cgggggtgac gtggggggt
//
LOCUS       DASWNJ010000318         1935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_288704, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000318 DASWNJ010000000
VERSION     DASWNJ010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288704"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VGB85_05640"
     CDS             complement(<1..413)
                     /locus_tag="VGB85_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEY0133537.1"
                     /translation="MTMRLAVMAGGPGSSDALDVTSCEPDESNPNEPSADPPRAPSVT
                     DEVRRNLLRSRTVLIFGEIDSALAQATTAQLLALASDSDAPIRIVLHSPGGHVESGDT
                     IHDVIRFIRPEVKMIGTGWVASAGALIFVAAARERR"
     gene            479..1318
                     /locus_tag="VGB85_05645"
     CDS             479..1318
                     /locus_tag="VGB85_05645"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY0133538.1"
                     /translation="MADQTEEQLVRAGSVSRRHGAPPWQVDAGDRTSLHVVVDGSFRL
                     TRDAAPTLALEPGDVVLVAAPQAHSLRCLATGATRHAELLSASYDATPLPTPVVHLSA
                     ADVRRSPGLAALVSLLRAALAEPGAAQDGLARSLLAPLLTYTLHCHDVVGDGPAARAH
                     DRRIARALEVMQARPTERWTVAAHARAAGLSRAAFARRFLAELGVPPLRHLTELRMRR
                     AARRLAESDDSLAAIAAEVGYDSEFAFSRAFKRHMGEAPGGFRRRSRVEGTFAPPIVC
                     LAA"
     gene            1500..>1935
                     /locus_tag="VGB85_05650"
     CDS             1500..>1935
                     /locus_tag="VGB85_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133539.1"
                     /translation="MPRLPLLAALLVCAACPARSTSSTQVAAPAEPAGDAVRATPGPA
                     RGVRTVQERTVFHLHKFLLRVGVEVDTYVPDGAGGVEAKASFGFQDRGAQVTLAARYT
                     LAADGSPEASLAWGRTSRISSIDEAITRVADDTYDVLRLGEDA"
BASE COUNT          219 a          729 c          724 g          263 t
ORIGIN      
        1 cggcgctccc gcgcggccgc gacgaagatc aacgcgccgg cgctggcgac ccacccggtg
       61 ccgatcatct tcacctcggg gcggatgaag cggatcacgt cgtggatcgt gtcgcccgac
      121 tcgacgtgcc cgcccggcga gtgcagcacg atccggatcg gcgcgtcgct gtcgctggcg
      181 agcgcgagca gctgcgccgt ggtcgcctgc gcgagcgcgc tgtcgatctc gccgaagatc
      241 agcacggtgc ggctgcgcag caggttcctg cgcacctcgt cggtcacgct cggggcgcgc
      301 ggcgggtccg cgctcggctc gttcgggttc gactcgtccg gctcgcagga tgtcacgtcg
      361 agagcatccg acgagcccgg gccgcccgcc atgaccgcga ggcgcatcgt catacccgat
      421 cgtctcgacc tgctgccccg cgctggacaa gccgccccgg cgcgggcaac catcgcgcat
      481 ggccgaccag acggaggagc agctcgtgcg cgcaggttcg gtgtcccggc gtcacggtgc
      541 gccgccgtgg caggtcgacg ccggcgatcg cacgagcctg cacgtcgtcg tcgacgggtc
      601 gtttcgcctc acgcgcgacg cggcgccgac cctcgcgctc gagccgggcg acgtcgtgct
      661 cgtcgcggcc ccgcaggcgc acagcttgcg atgcctcgcg accggcgcga cacggcacgc
      721 cgagctgctc tcggccagct atgacgcgac gccgctgccg acgccggtcg tgcacctttc
      781 ggcggccgac gtgcggcgct cgccgggcct cgccgcgctg gtcagcctgc tgcgtgcggc
      841 gctcgccgag cccggcgcgg cgcaggacgg gctcgcgcgc tcgttgctcg cgccgctgct
      901 cacatacacg ttgcactgtc acgacgtcgt cggcgacggc cccgcggcgc gagcccacga
      961 ccggcggatc gctcgcgcgc tcgaggtcat gcaggcgagg ccgaccgagc gctggaccgt
     1021 cgcggcgcac gccagggccg ccggactgtc gcgcgccgcg ttcgcgcgcc gcttcctcgc
     1081 cgagctcggc gtcccgccgc tgcgtcacct gaccgagctc cgcatgcgac gcgcggcccg
     1141 gcggctcgcc gagagcgacg actccctggc cgcgatcgcg gccgaggtcg gctacgactc
     1201 cgagttcgcg tttagccgcg ccttcaagcg ccacatgggc gaggcgccgg ggggctttcg
     1261 ccgtcgcagc cgcgtcgaag gcacgttcgc cccgccgatc gtctgcctgg ccgcgtgagg
     1321 catttttgct gcctgcctgc cagtcggtca ccgcggcgag gagacgcgca gatcgccggg
     1381 ccagcgccgc ggcgatcgat ggcgatgcaa cttcatcgcg gcgcgccggg tgcctcgggc
     1441 ccgcggagcc ccgcgcgatc cggcttgcgc gcggcggggc gatccgatag ggcggctgca
     1501 tgcccaggct gccgctgctc gccgccctgc tcgtctgcgc cgcatgtccg gcgcggtcca
     1561 cctcgtcgac ccaggtcgcg gcgccggccg agcccgcggg ggatgcggtg cgcgcgacgc
     1621 cggggccggc gcggggcgtg cggacggtgc aggagcggac cgtgtttcac ctgcacaagt
     1681 tcctgctgcg ggtcggcgtc gaggtcgaca cgtacgtgcc ggacggcgcg ggcggggtcg
     1741 aggccaaggc gtcgttcggg ttccaggacc gcggggccca ggtgacgctc gcggcccgct
     1801 acacgctcgc ggcggacggc tcgccggagg cctcgctggc gtggggcagg acgtcgcgga
     1861 tctcgtcgat cgacgaggcg atcacgcgcg tcgccgacga cacctacgac gtgctgcggc
     1921 tcggcgagga cgcgg
//
LOCUS       DASWNJ010000319         2531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_147054, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000319 DASWNJ010000000
VERSION     DASWNJ010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147054"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="VGB85_05655"
     CDS             <1..1159
                     /locus_tag="VGB85_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0133540.1"
                     /translation="YKKVAPEIGLRFAWYPLAFLGGEIEGGAMPTMTDPVEGSMKTAA
                     RATLFNFRGHLIGQIPFWRITPFILVGGGMLGTTGALGTDVDPATHFGGGVKFYITHR
                     FMMRLDVRDVVAAKLRVDSGATNYPEVLLGLSLTLNRKKKPAEPDKDSDGDGFLDNVD
                     ACPVEPGIAPDGCPERDRDGDGFMDSQDACPDVPGIAPDGCPEKDRDGDGFLDSVDKC
                     PDDPGVEPDGCPIPDTDGDGILDPDDKCPTVPETKNGYQDEDGCPDEIPLQLAKFTGT
                     IKGIYFDLDKDTIKPKSRPVLDRAVAVLNEFNTIRIEISGHTDSTGSVAYNKDLSGRR
                     AASVKNYLVEHGIDTARIETRGAGPDEPVDTNKTAAGRAKNRRIEFTILVQ"
     gene            1385..2029
                     /locus_tag="VGB85_05660"
     CDS             1385..2029
                     /locus_tag="VGB85_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY0133541.1"
                     /translation="MNLRSSFLSVLGLAGVLSLSVGCDGDSKKAEPVKADAEVKAAEP
                     AKTEPVKTEPVKTEPTPTDIKAADTAAAGDTKPADAAGGDTKTGDAAGDTKAEDTKAG
                     DTKTGDTKTPTKTETKKPAETKTADKTPAIDGKPLYDSKCKVCHAADGKGTEAMKKNN
                     IPDMSDKGWQGKHSKAVVVKAIANGIDGSKMKAFKDKLKPEEIDAIATYVKKLK"
     gene            complement(2079..>2531)
                     /locus_tag="VGB85_05665"
     CDS             complement(2079..>2531)
                     /locus_tag="VGB85_05665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0133542.1"
                     /translation="GPSELVPLDLRRVAERLVELTQAEAGQKQVRLILDVPATPVNVR
                     GDADQLASLALNVAVNAFKALGPAGGTLRIAVADAHGEATLTLENDGPPLPEVERARL
                     FAPFYSDSQGTGLGLSIAARIAEQHGGSIKAENAGLGVRFMLDLPRLH"
BASE COUNT          467 a          890 c          848 g          326 t
ORIGIN      
        1 gtacaagaag gtcgccccgg agatcggcct gcgcttcgcc tggtatccgc tcgcgttcct
       61 cggcggcgag atcgaaggcg gcgcgatgcc gacgatgacc gacccggtcg aagggtcgat
      121 gaagaccgcc gctcgcgcga cgctgttcaa cttccgcggt cacctgatcg gccagatccc
      181 gttctggcgc atcaccccgt ttatcctcgt cggcggcggc atgctcggca cgaccggcgc
      241 cctcggcacg gacgtcgacc ccgccacgca cttcggcggc ggtgtgaagt tctacatcac
      301 ccaccgcttc atgatgcgcc tcgacgtccg tgacgtcgtc gccgccaagc tgcgcgtcga
      361 ctccggcgcg accaactacc ccgaggtgtt gctcggcctg tcgctgacgc tcaaccgcaa
      421 gaagaagccc gccgagcccg acaaggacag cgacggcgac ggcttcctcg acaacgtcga
      481 cgcttgcccg gtcgagcccg gcatcgcgcc cgacggctgc cccgagcgcg accgcgacgg
      541 cgacggcttc atggacagcc aggacgcgtg cccggatgtc cccggcatcg cgcccgatgg
      601 ttgcccggag aaggaccgcg acggcgacgg cttcctcgac agcgtcgaca agtgcccgga
      661 cgacccgggt gtcgagcccg acggctgccc gatcccggac accgacggcg acggcatcct
      721 cgatccggac gacaagtgcc cgacggtgcc ggagaccaag aacggctacc aggacgagga
      781 cggctgcccc gacgagatcc cgctgcagct ggccaagttc accggcacga tcaagggcat
      841 ctacttcgac ctcgacaagg acacgatcaa gccgaagtcg cgcccggtgc tcgaccgcgc
      901 ggtcgcggtg ctcaacgagt tcaacacgat ccgcatcgag atcagcggcc acacggacag
      961 cacgggttcg gtcgcctaca acaaggacct gtcgggtcgc cgcgcggcgt ccgtcaagaa
     1021 ctacctggtc gagcacggca tcgacaccgc tcgcatcgag acccgcggtg cgggtccgga
     1081 cgagccggtc gacaccaaca agaccgcggc cggtcgcgcc aagaaccgcc gcatcgagtt
     1141 cacgatcctc gtgcagtaaa ctacgcctcc gggtccacgc ccgggaggtc ccacagagcg
     1201 ccatcgtcgg ttcaccggcg gtggtgctct ccgtttaagc agcgggagat cggcggcggt
     1261 cgtcgttcgt acatggtccg gcaccgtgtc gcgcgcgcct ccgctccggc cgcgaacttg
     1321 tcgtccccgg gcactttccc tacactccct ccgctggccc ctggccacga aagagaaccc
     1381 acccatgaac cttcgcagca gtttcctctc cgtgcttggc ctggccggcg tcctcagcct
     1441 cagtgtgggt tgcgacggtg actcgaagaa ggccgagccg gtcaaggccg acgcagaagt
     1501 caaggccgcc gagcccgcga agacggagcc cgtcaagacg gagcccgtga agaccgagcc
     1561 gaccccgacc gacatcaagg ccgccgacac ggccgccgcc ggggacacca agccggccga
     1621 cgccgcgggt ggcgacacca agacgggcga cgccgcgggc gacaccaagg ccgaggacac
     1681 caaggccggg gacaccaaga ccggggacac caagaccccg acgaagaccg agaccaagaa
     1741 gccggccgag accaagacgg cggacaagac cccggcgatc gacggcaagc cgctctacga
     1801 ctcgaagtgc aaggtctgcc acgcggccga cggcaagggc accgaggcga tgaagaagaa
     1861 caacatcccg gacatgtccg acaagggctg gcagggcaag cactcgaagg ccgtcgtcgt
     1921 caaggcgatc gccaacggca tcgacggcag caagatgaag gccttcaagg acaagctcaa
     1981 gccggaagag atcgacgcga tcgcgaccta cgtgaagaag ctgaagtaat ccgcttcgcg
     2041 tgttcgagga cagccggccc atgcgccggc tgttcgcgtt aatgcaggcg cggcaggtcg
     2101 agcatgaagc ggacgccgag gccggcgttc tcggccttga tgctgccgcc gtgttgctcg
     2161 gcgatgcggg cggcgatcga cagaccgagg ccggtgccct ggctgtcgct gtagaacggc
     2221 gcgaacaggc gcgcgcgctc gacctcgggc aggggtgggc cgtcgttctc gagggtcaac
     2281 gtcgcctcgc cgtgggcgtc ggcgacggcg atccgcagcg tgccgccggc ggggcccagg
     2341 gccttgaacg cgttgacggc gacgttgagc gcgaggctgg cgagctggtc ggcgtcaccg
     2401 cggacgttca cgggcgtcgc gggcacgtcg aggatcagcc tgacctgctt ctggccggcc
     2461 tcggcctggg tcagctcgac gaggcgctcg gcgacgcggc gcaggtcgag cggcaccagc
     2521 tcgctcgggc c
//
LOCUS       DASWNJ010000320         1993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_358549, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000320 DASWNJ010000000
VERSION     DASWNJ010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358549"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..1727
                     /locus_tag="VGB85_05670"
     CDS             210..1727
                     /locus_tag="VGB85_05670"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HEY0133543.1"
                     /translation="MLSRVPVGVLTLLALLAPTARAENFVGPWAHVEPDGHDSHERPP
                     GWDTFAVPEPPPFWIAPEYPLAVAFIPADPNNFTPDGIESYDYVVVHTMQGSYNGTIS
                     WFQNPAAVVSSQYVMRSGDGEVTQMVLDKDKAYHVGTANRFALGIEHEGWVDDPDKWY
                     TWATYTSSARLTRWLAIKHDIPVDRQHIVAHSELPNQTHTDPGPGWNWNLYMALINEV
                     VPPSEIRGVAVDRSKPCTLTASVDTPIKRTAMPSHLQPAEDKCTVTAGTTLAYWHARR
                     DIDGHHRLFMPAGEGPCAGAGLDADGYIIAADWSALCPDEAIAAAGVTVTIDGAGPIT
                     TGPDGSFTFMAGGGAHTIDAAQDGVFEPASQPVELAGYPGARVVIALEPVPAPDPTTD
                     VTTDPTTDPTTDPSAGSDTEPGTAETAGDGTGIGEAGETAGDASGPGDEPTSGPGETT
                     GAPTEGSADNGGAPALPDGYGQDDGCGCTTSGSPASAPALLLLGLLAIPRRRRRA"
BASE COUNT          306 a          790 c          662 g          235 t
ORIGIN      
        1 cgccaaggac atcgagaagg accccgccgc cgccggcgag ctgcgcgaga agtccgccaa
       61 gaagggcctc aagaccggca gcgtgcccgt catcgacgtc cgcggcgagc tgatggtcgg
      121 cttcgatcgc gcgcgcctcg agtccctgct gtagcggcgc catcctgccc gccgttgcgc
      181 gcgggcggcc cgggggatag cgtggagtca tgctgtctcg cgtcccggtc ggtgtcctga
      241 cgttgctcgc cctgctcgcc ccgaccgcgc gcgccgagaa cttcgtcggt ccgtgggccc
      301 acgtcgagcc cgacggccac gactcgcacg agcgtccgcc cggctgggac accttcgccg
      361 tgcccgagcc gccgccgttt tggatcgcgc ccgagtaccc gctcgccgtc gccttcatcc
      421 cggccgaccc caacaacttc acccccgacg gcatcgagag ctacgactac gtcgtcgtgc
      481 acacgatgca gggctcgtac aacggcacga tctcgtggtt ccagaacccg gcggccgtcg
      541 tctcgtcgca gtacgtcatg cgctcgggcg acggcgaggt cacgcagatg gtgctcgaca
      601 aggacaaggc gtaccacgtc ggcaccgcca accgcttcgc gctgggcatc gagcacgagg
      661 gctgggtcga cgatccggac aagtggtaca cgtgggcgac gtacacgtcc tcggcccgcc
      721 tgacccgctg gctggcgatc aagcacgaca tccccgtcga tcgccagcac atcgtcgccc
      781 acagcgagct gcccaaccag acccacaccg accccggccc gggctggaac tggaacctct
      841 acatggcgct gatcaacgag gtcgtgccgc cctcggagat ccgcggcgtc gccgtcgacc
      901 gcagcaagcc gtgcacgctg accgcgagcg tcgacacgcc gatcaagcgc accgcgatgc
      961 cgagccacct gcagccggcc gaggacaagt gcaccgtcac ggccggcacc acgctcgcct
     1021 actggcacgc gcgccgcgac atcgacggcc accatcgcct gttcatgccc gcgggcgagg
     1081 gtccgtgcgc cggcgccggc ctcgacgccg acgggtacat catcgccgcc gactggagcg
     1141 cgctgtgccc cgacgaggcg atcgcggccg ccggcgtcac cgtcacgatc gacggcgccg
     1201 gcccgatcac gaccggcccc gacggcagct tcaccttcat ggccggcggc ggcgcgcaca
     1261 cgatcgacgc cgcgcaggac ggcgtgttcg agcccgcgtc ccagcccgtc gagctcgcgg
     1321 gctaccccgg cgcccgggtg gtgatcgccc tcgagccggt gcccgcccct gacccgacca
     1381 ccgacgtcac caccgacccg accaccgacc cgaccaccga ccccagcgcc ggatccgaca
     1441 cggagccggg caccgcagag accgcgggcg acggcaccgg catcggcgag gccggcgaga
     1501 cagccggcga cgcctccgga cccggcgacg agccgaccag cgggccgggc gagaccaccg
     1561 gtgccccgac cgagggcagc gccgacaacg gcggcgcgcc ggccctgccc gacggctacg
     1621 gccaggacga cggctgcggc tgcacgacct cgggatcgcc cgcgtcggcg cccgcgctgc
     1681 tgctgctcgg cctgctggcg atcccgcgac ggcgccggcg cgcctgagcc tgtcccaacg
     1741 gacaggtgac caccgaccgg cgcaaggctg gtgcgccgcc gcgtgagcaa gctcgggcgg
     1801 ccgtgaaagc acgcccggat tctggtacga acgggatacg cctgcgttcg ccgaaatctc
     1861 gcccgccacc ctgggcaccg ccgagaccta ccggttgatg accgacctgg tcgcgccgcg
     1921 gccgatcgcg tgggtgtcct cggaggacgg ccagggccgg cgcaacctcg cgcccttcag
     1981 ctacttccag gcc
//
LOCUS       DASWNJ010000321         2237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_77139, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000321 DASWNJ010000000
VERSION     DASWNJ010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77139"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VGB85_05675"
     CDS             <1..362
                     /locus_tag="VGB85_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEY0133544.1"
                     /translation="RGMPYAVAEISRALGSSHPLAHRGIDSLAHAQREDGGWAHGPDE
                     ASTPSATGLALTAVARRHPARIEAALRYLVDTQRPDGTWPGTADMYGPRPLLSHFTTH
                     TQAFVVGGILAATSVSA"
     gene            430..1308
                     /locus_tag="VGB85_05680"
     CDS             430..1308
                     /locus_tag="VGB85_05680"
                     /inference="COORDINATES: protein motif:HMM:NF040195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010333 - terpene synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0046246 - terpene biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terpene synthase family protein"
                     /protein_id="HEY0133545.1"
                     /translation="MTRVAAAVDPTLLGRLLEWTQGFAGIEAHHAADGLFIGAAFRHD
                     DLPDDPDAVFLVGLVITFWFWFDDRADRFLCAPETWDALIALGADPTHVTTIDTPETR
                     FFQRLAGALRERAGDPAEHRWWLSCSADVFRSMRAEEQMTRAGVSLSYAEALEIGADS
                     TALTSILSAADLAHGMNRAARGADQRLARVERYMCLSQRLLNDLNSAEKERREGHAGR
                     VSNIVLLLEATMSPAQARAFVETQRHGYERMTLESIALLGPDDGFGRLIHDGMRNIRR
                     WYETGPVRYENRGGAA"
     gene            1305..>2237
                     /locus_tag="VGB85_05685"
     CDS             1305..>2237
                     /locus_tag="VGB85_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0133546.1"
                     /translation="MSSIINAKHTTDPRSDLPGPRGLPLIGSLPALLREGPFEYVGRT
                     WREHGDMFQIPTLGRDRIVFISHPNELDRLLSHPEIYIKDRNYDPARPLFGEGLVTSN
                     GDSWLAQRKLIQPMFNKTMLRSYAPLMVMCVREMLARWEKKRAAGEPIELAEEMGRLT
                     HHVVGMTLFGQNLTDSAADAAQAVTDSLHLAGARVNRGALVLPLWMPTPSNFRFRKAI
                     EILDRLVYGMIARARSTPAHPDEPPTLLRRLVDVRDEAGDAMSDRQLRDELLTHYVAG
                     QETTALVMTWTLHLLSSHKEVAARIRSESAGLDDS"
BASE COUNT          346 a          866 c          721 g          304 t
ORIGIN      
        1 accgcggcat gccctacgcc gtcgcggaga tcagccgcgc gctcgggtcc tcgcacccgc
       61 tcgcccaccg cggcatcgac tccctcgccc acgcccagcg cgaggacggc ggctgggccc
      121 acggacccga cgaggcgagc acgccgagcg cgaccggcct ggccctgacc gcggtcgccc
      181 gccgccaccc ggcccgcatc gaggctgccc tgcgctacct ggtcgacacg cagcgccccg
      241 acggcacctg gcccggcacc gccgatatgt acggaccccg cccgctgctg agccacttca
      301 cgacgcacac ccaggcgttc gtcgtcggcg ggatcctggc cgcgacgtcc gtcagcgcct
      361 gagcggcccg tataatcatc gcatgacatc gatcgtgtat acatattgcg cgagcgaggc
      421 gggggcacga tgacgagggt cgcggccgcc gtcgatccca cgctgctcgg gcgcctcctc
      481 gaatggacgc agggcttcgc gggcatcgag gcgcaccacg ccgccgacgg gctgttcatc
      541 ggcgcggcgt ttcgccacga cgacctgccc gacgaccccg acgcggtgtt cctcgtcggc
      601 ctggtgatca cgttctggtt ctggttcgac gaccgcgccg atcgcttcct gtgcgccccc
      661 gagacctggg acgcgctgat cgccctcggc gccgatccga cccacgtcac cacgatcgac
      721 acccccgaga cccgcttctt ccagcggctc gccggggcct tgcgcgagcg cgcaggcgac
      781 cccgccgagc accgttggtg gctgtcctgc tcggccgacg tgttccgctc gatgcgcgcc
      841 gaggagcaga tgacgcgcgc cggcgtcagc ctgtcctacg ccgaggccct cgagatcggc
      901 gccgacagca cggcgctgac gagcatcctg tcggcggccg acctcgccca cggcatgaac
      961 cgggcggccc gcggcgccga ccagcggctc gcccgcgtcg agcgctacat gtgcctgtcg
     1021 cagcgcctgc tcaacgacct caacagcgcc gagaaggagc gccgggaggg ccacgccggg
     1081 cgcgtctcca acatcgtgct cctgctcgag gcgacgatgt cgccggcgca ggcgcgggcc
     1141 ttcgtcgaga cccagcgcca cggctacgag cgtatgacgc tcgagagcat cgcgctcctc
     1201 ggccccgacg acggcttcgg gcgcctgatc cacgatggaa tgcgtaatat ccggcgctgg
     1261 tacgagaccg ggcccgtacg ctacgagaac cgaggaggcg ccgcatgagc tcgatcatca
     1321 acgcgaaaca cacgaccgac ccgcgcagcg acctccccgg accccgcgga ttacccctca
     1381 tcggctcgct gccggcgctc ctgcgcgagg gccccttcga gtatgtcggt cgcacgtggc
     1441 gcgagcacgg cgacatgttt cagatcccca cgctcgggcg cgaccgcatc gtcttcatca
     1501 gccacccgaa cgagctcgac cgcctgctgt cccacccgga gatctacatc aaggacagga
     1561 actacgaccc cgcgcgcccg ctgttcgggg aggggctcgt caccagcaac ggcgacagct
     1621 ggctcgccca gcgcaagctg atccagccga tgttcaacaa gaccatgctg cgcagctatg
     1681 cgcccttgat ggtcatgtgc gtgcgggaga tgctcgcgcg ctgggagaag aagcgcgccg
     1741 ccggcgagcc gatcgagctc gccgaggaga tgggccggct cacgcaccac gtcgtcggca
     1801 tgacgctgtt cgggcagaac ctcaccgaca gcgcagcgga cgcggcgcag gcggtcaccg
     1861 acagccttca cctcgccggc gcgcgggtca accgcggcgc cctcgtgctg ccgctgtgga
     1921 tgccgacccc gtccaacttc cggtttcgca aggcgatcga gatcctcgac cgcctcgtct
     1981 acggcatgat cgcccgcgcc cgcagcacgc ccgcgcaccc cgacgagccg ccgaccttgc
     2041 ttcgccgtct cgtcgacgtg cgcgacgagg ccggcgacgc catgtcggac cgccagctgc
     2101 gcgacgagct gctgacccac tacgtcgccg gccaggagac caccgcgctg gtgatgacgt
     2161 ggaccctgca cctgctctcg agccacaagg aggtcgccgc gcgcatccgc agcgagtctg
     2221 ccggattgga cgactcc
//
LOCUS       DASWNJ010000322         1763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_6396, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000322 DASWNJ010000000
VERSION     DASWNJ010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6396"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VGB85_05690"
     CDS             <1..245
                     /locus_tag="VGB85_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133547.1"
                     /translation="YFGQGFNARKLPQSADLERVEKAMLNERLQLATAPSRRGAYRKS
                     DGFALIGRIDPARLRQRCSHAAALFAELALRTAASG"
     gene            complement(249..998)
                     /locus_tag="VGB85_05695"
     CDS             complement(249..998)
                     /locus_tag="VGB85_05695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0133548.1"
                     /translation="MPLPSLYTDHAAWFHLLSPPEEYVEEAAFYTRLIQGASARPPRT
                     LLELGSGGGSMASQYVQHFEATLSDLSPAMLDLSRQIHPTLEHIVGDMRTLRVGREFD
                     AVFVHDAVCYLTSEDDLRQCMTTAWTHLRPGGVAVFAPDHTQENLEPGTDHGGHDSPD
                     GARGLRYLEWTHAAEPGATWHAVDYILAFNEAGKPLRVEHDRHIHGLFSRDTWIRLLR
                     ETGFENIEPRPLEHSEVPPGSTEVFVAVRPE"
     gene            complement(1006..1737)
                     /locus_tag="VGB85_05700"
     CDS             complement(1006..1737)
                     /locus_tag="VGB85_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0133549.1"
                     /translation="MSPLPLQVVIADDELLARRRLARLLGDLPAVHVVAECESGQALL
                     ALLARGAHDVDLVLLDIHMPGLSGLETGALLPEDGPYVIFTTAHSEHALAAFDLGAVD
                     YLLKPIDAVRLGKALARARHRLGPQPAAPAPALVRLPIATRQGIRLIDPGDLSHATFD
                     GELVTLHTRGGPLLTDFSLQDLEDRLPAGLFERVHRRALINLERLACLDPLETGGYTA
                     RMQDGGLVPISRQAARRLRRRLGLS"
BASE COUNT          248 a          531 c          725 g          259 t
ORIGIN      
        1 aatatttcgg ccagggcttc aatgcaagga agctcccgca gtcggccgac ctcgagcgcg
       61 tggagaaggc catgctcaac gagcggttgc agctggcgac ggcgccgtcg cggcgcgggg
      121 cataccggaa atccgacggc ttcgcgttga tcgggcggat cgacccggcg cggctgcgtc
      181 agcggtgctc gcacgcggcg gcgttgttcg ccgagctggc gctgcgcacc gcggcgagcg
      241 gctgacggtc actccgggcg cacggccacg aacacttcgg tcgatcctgg cgggacctcg
      301 ctgtgctcga gcgggcgggg ttcgatgttt tcaaagccgg tctcgcggag caggcggatc
      361 caggtgtcgc gggagaacag gccgtggatg tggcggtcgt gctcgacgcg cagcggtttg
      421 ccggcctcgt tgaaggcgag gatgtagtcg acggcgtgcc aggtggcgcc cggttcggcg
      481 gcgtgggtcc actcgaggta gcgcaggccg cgggcgccgt cggggctgtc gtgaccgccg
      541 tggtcggtgc cgggctcgag gttctcttgt gtgtggtcgg gggcgaacac ggcgacgccg
      601 ccggggcgca ggtgggtcca tgcggtcgtc atgcactggc gcaggtcgtc ctcgctggtc
      661 aggtagcaga cggcgtcgtg tacgaacacg gcgtcgaact cgcggccgac ccggagcgtg
      721 cgcatgtcgc cgacgatgtg ctcgagcgtg gggtggatct ggcggctcag gtcgagcatc
      781 gcgggcgaga ggtcggagag ggtcgcctcg aagtgctgga cgtattgcga ggccatgctg
      841 ccgccgccgg agccgagctc caacagggtc cgcggcgggc gggcgctggc tccctggatc
      901 aggcgcgtat aaaacgcggc ctcctcgacg tactcctccg gtgggctgag gaggtggaac
      961 caggcggcgt gatcggtgta caggctcggg agcggcatgg ccgggtcagg ataacccgag
     1021 gcggcgtcgc aatcggcggg cggcctgccg cgagatcggg accaggccgc cgtcctgcat
     1081 ccgcgccgtg tagccgccgg tctccagcgg gtcgaggcag gcgagccgct cgaggttgat
     1141 cagggcgcgt cgatgcacgc gctcgaacag gccggccggg aggcggtcct cgaggtcctg
     1201 cagcgagaag tcggtcagca gcgggccgcc acgggtgtgc agggtgacga gctcgccgtc
     1261 gaaggtcgcg tgcgacagat cgccggggtc gatcaggcgg atcccctggc gcgtggcgat
     1321 cggcagacgg accagcgcgg gcgccggggc ggccggctgt gggccgaggc ggtgacgcgc
     1381 gcgcgcgagc gccttgccga gccgcacggc gtcgatcggt tttaataagt agtcgacggc
     1441 gccgaggtcg aaggcggcga gggcgtgctc gctgtgcgcg gtggtgaaga tgacgtaggg
     1501 gccgtcctcg ggcagcaagg cgccggtctc gaggcccgag aggccgggca tgtggatgtc
     1561 gagcagcacg aggtcgacgt cgtgcgcgcc gcgggcgagc agggcgagca gcgcctggcc
     1621 ggactcgcac tctgccacca cgtgcacggc cggcaggtcg cccaggagac gcgcgaggcg
     1681 acggcgggcg agcagctcgt cgtcggcgat gaccacctgc agcgggagcg gtgacatgtc
     1741 ttttgggtcg gattaggggc tag
//
LOCUS       DASWNJ010000323         2877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_500063, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000323 DASWNJ010000000
VERSION     DASWNJ010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500063"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2554)
                     /locus_tag="VGB85_05705"
     CDS             complement(<1..2554)
                     /locus_tag="VGB85_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0133550.1"
                     /translation="MERRLGHGGMGSVYLARHRLLGRWVALKVLSPRADRTSGQDARL
                     LREAQALANIRHKNVVQVHQVDNDGEQTVIEMEYVDGPTLRAWQAGRSWREVVAAYAE
                     AGDGLAAIHQAGFVHRDIKPDNLLRDAEGLVKVADLGLAISGESVASEAAPSVGPHPT
                     SSRLTADGALVGTLAYMAPEVIVGQPATAASDLFSLAASLYESIHGVLPFHGDTAEAH
                     AAAIHAGTLSQSLHRPPLPKWLDLTLHRALDPDPERRHGAVQQFVRELRRGLRRRRDR
                     VMLALAGLTLVGLPVLTLWLAAPTHDPCPEAGRPFLELWDEVTRAELKVRVGTTPAPP
                     IQRSFDLLTRTLDSTTRSLSDTASTLCRADASVTAQASHVGDGLDLRFRQRACLDHTF
                     RDLRALVNRLRATEDDLARHYVDAVGTLEALPRCDNPRDLIHWSVSPGMAELDAGIAE
                     VLANAAALESTGDYIGAASLAESAVRTSAHASPQYQAEALYRLGHILGEQHRYTEAYA
                     TLVKAREFAFGIGHDELFCRTGVFLAKLTANVGLDPATSTLELGLANACRKRIDARSI
                     LFNADLLEAAGLLALAAADPAAAIRWHGEALALRRDHLGDLNHPTLKSVHNLANALGE
                     AGETEAARQRYDEVLSGYEQLFGRDNVEVADVLFDYGDLLLLTDPEKSSSLLARALEI
                     FTRPSAPRPAAAAKTHIQLARGELAKGPVADLDAVADHLSQARTLQRDATLDPNHPDR
                     VALLQVEGELALKTPDYPAAARAYARATQMLARRGAADAKLHESILGEIDAAYGSGDF
                     SRIVRHAHDEGPPLVAHLRLLEPALERGRSAWYIGESLARAGFPEDGAVYLQI"
     gene            2534..2680
                     /locus_tag="VGB85_05710"
     CDS             2534..2680
                     /locus_tag="VGB85_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133551.1"
                     /translation="MPEPTLHVPLAKSDRLGLCAGERAAKLRQVELTVEVLERCRAVT
                     PEQD"
BASE COUNT          451 a          890 c         1081 g          455 t
ORIGIN      
        1 cgatctgcag atagacggcg ccatcctcgg ggaagccggc ccgggcgagg ctttcgccga
       61 tgtaccaggc cgagcggcct cgctccaggg cgggttccag caatcgcagg tgcgccacga
      121 gcggaggtcc ctcgtcgtgg gcgtgtcgga cgatccggct gaagtcgccg gagccatacg
      181 cggcgtcgat ctcaccgagg atgctctcgt ggagcttggc gtcggcggcg ccgcgacgag
      241 ccagcatttg ggtcgctcgc gcgtaggctc gcgcagcggc ggggtagtcc ggggtcttca
      301 gcgccagctc cccctcgact tggagcaggg cgacgcggtc gggatggttg gggtcgagcg
      361 tcgcgtcgcg ctggagggtt cgggcctggc tcagatggtc cgccaccgca tccaggtccg
      421 ccaccgggcc cttggcgagt tcaccgcgcg cgagttgaat gtgggtcttc gcagcggcgg
      481 cggggcgggg agccgacgga cgggtgaaga tctcgagggc gcgcgcgagc agcgaggatg
      541 acttctccgg gtccgtcaac agcaggagat caccatagtc gaacaggaca tcggcgacct
      601 cgacgttgtc ccgcccgaac agttgttcgt acccggacag cacctcgtcg tagcgctggc
      661 gcgctgcttc ggtctcgccg gcctcgccga gcgcgttggc gaggttgtgt acagatttga
      721 gggtgggatg gttcaagtca ccgaggtgat cacgtcgtag cgccagagct tcgccgtgcc
      781 accggatcgc agccgccgga tcggcggcgg ccagggcgag caggcccgcg gcctcgagca
      841 ggtcggcatt aaaaagaatc gagcgcgcgt caatccgctt gcggcacgcg ttggcgaggc
      901 cgagctccag ggtgctggtc gccggatcga gaccgacgtt ggccgtgagc ttggccagga
      961 acacgccagt acgacaaaac agctcgtcgt gaccgatccc aaaggcgaac tcgcgcgcct
     1021 tcacgagggt cgcgtaggcc tcggtgtaac ggtgctgctc gccgaggata tgtccgaggc
     1081 gatagagcgc ctcggcttga tattgtgggg aggcatgagc gctggtcctc acggcgctct
     1141 cggcgaggct ggcggcgccg atataatcgc cggtgctctc cagagccgcg gcgttcgcca
     1201 acacctcggc gatacccgcg tcgagctccg ccatgcccgg cgacactgac cagtggatga
     1261 gatcgcgcgg attgtcgcag cgaggcagcg cctcgagggt gcccacggcg tcgacgtagt
     1321 ggcgggctag gtcgtcctcg gtggcgcgaa ggcgattcac gagggcacgt agatcacgga
     1381 atgtgtggtc gagacatgcg cgctggcgga aacgaaggtc gaggccgtcg ccgacgtgcg
     1441 atgcctgtgc ggtcaccgat gcgtcggccc tgcagagcgt gctggcggtg tcggatagtg
     1501 agcgggtggt gctgtcgagc gtgcgtgtga ggaggtcgaa cgagcgctgg atcggcggcg
     1561 cgggcgtggt gcccacgcgc acctttagct cggcccgggt gacctcgtcc caaagctcga
     1621 ggaagggcct tccggcttca ggacacgggt cgtgcgtagg cgcggcgagc cacagggtca
     1681 ggacagggag tccgacgagg gtgagcccgg ccagcgcgag catgacccga tcgcgacggc
     1741 ggcgcaggcc gcgacgcagc tcgcggacaa attgctgtac ggcgccgtgg cgtcgctctg
     1801 gatccgggtc gagggcgcga tggagcgtca ggtcgagcca cttgggtagt gggggtctgt
     1861 ggagggactg cgagagcgtg ccggcgtgga tcgccgcggc gtgcgcctcc gcggtgtcac
     1921 cgtggaaggg gaggacccca tggatcgact cgtagagcga ggcggccagg ctgaacagat
     1981 cgctggcggc cgtcgccggc tgtccgacga tcacctccgg cgccatgtaa gcgagggtgc
     2041 cgacgagcgc gccgtcggcg gtgaggcggc tggaggtcgg gtgcggacca acagagggag
     2101 ccgcctccga cgccacactt tcacccgaga tggcgagccc gaggtcagcg accttgacga
     2161 ggccctcggc gtcgcgcagc aggttgtcgg gcttgatgtc acggtggacg aagcctgcct
     2221 ggtggatcgc cgccagaccg tcgccggctt ccgcgtacgc cgcgactacc tcccgccagg
     2281 atcgaccggc ctgccaggct cgcagtgtcg ggccgtcgac gtactccatc tcgatgaccg
     2341 tctgctcacc atcgttgtcg acctggtgga cctggacgac gttcttgtgg cgaatgttgg
     2401 cgagcgcttg cgcctcacgg aggaggcgcg catcctggcc actggtccgg tcggcccggg
     2461 ggctgagcac tttgagcgcg acccaccggc ccagcagtcg gtgcctggcc agatagaccg
     2521 aacccatgcc gccgtgcccg agccgacgct ccacgtccca ctggcgaaat cggaccggct
     2581 cggcctctgc gctggcgagc gcgcggcgaa gctgcgccag gtggagctga ccgtcgaggt
     2641 cctcgagcgg tgtcgggcgg tcacgccgga gcaggactga ggcgtcgtca ccagaacttg
     2701 tagggggcgt agaactcatg agcggcgcac cgggcgagcg ggacgctgat gatcctagtc
     2761 gctccccgag cggcttcgat cctccggtac ggtcaaaaat aattggcccg tgattcgctg
     2821 gtgtaaggga gggggagttg gaacgaccgc cctcgaaggt caggcgcttg gagccgc
//
LOCUS       DASWNJ010000324         2072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_465559, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000324 DASWNJ010000000
VERSION     DASWNJ010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465559"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1704)
                     /locus_tag="VGB85_05715"
     CDS             complement(<1..1704)
                     /locus_tag="VGB85_05715"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY0133552.1"
                     /translation="MADRIGELLVRENLISLAQLKRAQADQRTTGKRLSYSLSRLGIL
                     GERELADFLSAQYGVPWMSLTDYEIDPKVVELVPRAVASKHTAIPVQRAGSTLVVAMA
                     DPSNIYAIDDLKFITNLNVEPVVSTETAIEDAIARYYDRSGPAAYADVLGALNLERGD
                     GGERERAALPSAGLPESDQAIRLCNRVLLQAVSRGATQVHVEPSERGLRIRFRVDGVL
                     HPESELPIGLRSAVTTRFKLMAGLDVAERRTPQEGQIRGFHAGKDRELDVRVATLPTA
                     TGEQLVLRLFDRSTLPRDLASIGLDGEQLRQVRAALQLAQGLTLVVGPSGSGTSTTLY
                     AALAELAGDSVSVATVEDPVEFTLPGCTQVQVHKDGGLGVAAAVHALLRQDPDIIMVG
                     ALPDHEAVEAAVRAAVSGRRVLTTLPTGDTAGALTRLVHLGVEPYLVAASVTLVIAQR
                     LLRRLCNECASEDPSATAQRLIDAGMSPQQARECRPRRARGCKACVGGYRGRVPLFEV
                     LPVGPALQERIAEGAFREDLYYRLQVFLIHLPPLRERSGDIALLATHLIDEISRREGV
                     VK"
BASE COUNT          294 a          771 c          736 g          271 t
ORIGIN      
        1 cttgacgacg ccctcgcggc gcgagatctc gtcgatcagg tgggtcgcga ggagcgcgat
       61 gtcgccgctg cgctcgcgca gcggcggcag gtggatcagg aagacctgca ggcgatagta
      121 cagatcctcg cggaaggcgc cctcggcgat gcgctcctgc agcgccgggc caaccggaag
      181 cacctcgaac agcgggacgc gcccgcggta gccgccgaca cacgccttgc acccgcgagc
      241 gcgccgtggt cgacactcgc gggcctgctg cgggctcatg ccggcgtcga tcaggcgctg
      301 cgccgtcgcc gaggggtctt cgctggcgca ctcgttgcac agccgccgca gcaggcgctg
      361 cgcgatcacc agggtgaccg aggccgcgac caggtagggc tcgacgccga ggtgtacgag
      421 ccgcgtcagc gcgcccgcgg tgtcgccggt cggcagcgtc gtcagcaccc gtcgcccgga
      481 gaccgcggcc cgcaccgcgg cctcgaccgc ctcgtgatcc ggcagcgcgc cgaccatgat
      541 gatgtccgga tcttgccgca gcagcgcgtg cacggccgcc gcgacgccga gcccgccgtc
      601 tttgtgcacc tgcacctgcg tgcagccggg cagcgtgaac tcgaccggat cctcgacggt
      661 cgccacgctg acgctgtccc ccgcgagctc ggcgagcgcc gcgtacagcg tcgtgctcgt
      721 gccgctgccg ctcgggccga cgacgagcgt caggccctgc gcgagctgca aggccgcgcg
      781 cacctggcgg agctgctcgc cgtcgaggcc gatgctggcg aggtcacgcg ggagagtgct
      841 gcggtcgaac aggcgcagca ccagctgctc gccggtcgcg gtcggcaggg tcgccacgcg
      901 gacatcgagc tcgcggtcct tgccggcatg gaacccgcgg atctggccct cctgcggcgt
      961 gcgccgctcg gcgacgtcga gcccggccat caatttaaaa cgcgtggtca cggcgctgcg
     1021 cagcccgatc ggcagctcgg actccgggtg cagcacgccg tcgacgcgga accggatcct
     1081 caacccgcgc tcggacggct cgacgtgcac ctgcgtcgcg ccgcggctga ccgcctgcag
     1141 cagcacgcgg ttgcacagcc ggatcgcctg gtccgactcc ggcaggccgg ccgagggcag
     1201 cgcggcccgc tcgcgctcgc cgccgtcgcc gcgctcgagg ttgagggcgc cgagcacgtc
     1261 ggcgtaggcg gcgggcccgc tgcgatcgta gtaccgggcg atcgcgtcct cgatcgccgt
     1321 ctcggtgctg accaccggct cgacgttgag gttggtgatg aacttgaggt cgtcgatcgc
     1381 gtagatgttg ctcgggtcgg ccatcgcgac cacgagcgtc gagccggcgc gctgcacggg
     1441 gatcgcggtg tgcttgctgg cgaccgcccg cggcaccagc tcgaccacct tcggatcgat
     1501 ctcgtagtcg gtcaggctca tccatggcac gccgtactgc gccgacagga agtcggccag
     1561 ctcgcgctcg ccgaggatcc cgaggcgcga caggctgtac gacagccgct tgccggtcgt
     1621 gcgctgatcg gcctgggccc gcttgagctg ggcgagcgag atgaggttct cgcggaccag
     1681 gagttcgccg atgcggtctg ccatgccgga aagaacggaa gaacgggaga acgatctacc
     1741 ctgggggcgc atcagggtac agccccggcc cggacccgcc aagccggaca ggtgcccagg
     1801 cgagaaaact tgcacgcgca agccctcgcc ccgccgccat gttctcgcct atgctccgcc
     1861 gccgatgccc gaccgcgacc gacccgcccc gcgctccgct ccacccgctc gcggcgaccg
     1921 tggcggtgac tccgacagga gccgcgaccg ggacaaggag cgggctcccg cccgcagcgg
     1981 agagcgggct cccgcccgca gcagcgggcc gcgagaccac aacatccgcc ccgtcgagcg
     2041 cccggccgac atccgttcgc cggccccgcg ac
//
LOCUS       DASWNJ010000325         1568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_217723, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000325 DASWNJ010000000
VERSION     DASWNJ010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217723"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VGB85_05720"
     CDS             <1..877
                     /locus_tag="VGB85_05720"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEY0133553.1"
                     /translation="ARAGGSLDALWLATEQVFAELVRTYAADAAAAEAILHNPIYTAM
                     ARNLGGALEYAAMAKLQMLHAARSYDLIVLDTPPTANAIDFLSAPERIHELATNPAAR
                     LLTGGGRLGARLLGIGNSLVLRTLSSLAGSSFLTELGAFLRDFSTVLAAFQARAGDFQ
                     SLLTSPETGVVLATSASPFSVREALDFLKVLRERGLRVDSVVLNRVLPPFPPAPDAEV
                     LRAALGPVPEDSLQRVLATYAGLSAQGQQGAQARDAFASAHPGLPLWLLPQREPPPTS
                     LTDLQELVTQFVRAA"
     gene            complement(831..>1568)
                     /locus_tag="VGB85_05725"
     CDS             complement(831..>1568)
                     /locus_tag="VGB85_05725"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEY0133554.1"
                     /translation="DWIDATRGLATIAALNDPEQRTAARTIHEGDALAWLAARGELAG
                     CSLVTSLPDVSELAMTLPAWRAWFIAAAEAVLRACPPDGAVIFYQTDIKHAGVWVDKG
                     HLVQIAGDNCGASLWWHKIACRKPAGTITFGRPAYAHLLCFGREPLREPDGAADVFDT
                     GAMTWSRAMGVQACVVACEFIRRRTPSRVVVDPFCGRGTVLAVANAVGLDAVGVELSP
                     RRCRNARNLQISIKPPARTGSPAPADP"
BASE COUNT          212 a          630 c          510 g          216 t
ORIGIN      
        1 cgcgcgcgcc gggggcagcc tcgacgcgct gtggctggcg accgagcagg tgttcgccga
       61 gctggtgcgc acctacgcgg ccgacgcagc cgcggccgag gcgatcctcc acaacccgat
      121 ctacacggcg atggcccgca acctcggcgg cgcgctcgag tacgcggcga tggccaagct
      181 gcagatgctg cacgccgcgc gcagctacga cctcatcgtg ctcgacacgc ccccgaccgc
      241 caacgcgatc gacttcctca gcgcccccga gcgcatccac gagctcgcca ccaaccccgc
      301 cgcccgcctg ctgaccggcg gcggccgcct cggcgcccgc ctgctcggca tcggcaacag
      361 cctggtcctg cgcaccctct cgtcgctcgc cggcagctcc ttcctcaccg agctcggcgc
      421 cttcctgcgc gacttctcga ccgtgctcgc cgccttccag gcccgcgccg gcgacttcca
      481 gtcgctgctg acctcccccg agaccggcgt cgtgctcgca acctccgcct cgccgtttag
      541 cgtccgtgag gccctcgatt ttttgaaggt gctgcgcgag cgcggcctgc gggtcgactc
      601 cgtcgtgctc aaccgcgtgc tcccgccgtt cccgcccgcg cccgacgccg aggtcctgcg
      661 cgccgccctg ggacctgtcc ccgaggacag cttacaaagg gtgctcgcga cctacgccgg
      721 cctgtcggcg cagggccagc agggcgcgca ggcccgcgac gcgttcgcca gcgcgcaccc
      781 gggcctgccg ctgtggttgc tgccgcagcg cgagcccccg ccgacctcgc tcacggatct
      841 gcaggagctg gtgacccagt tcgtgcgggc ggcttgatcg agatctgcag gttgcgggcg
      901 ttgcgacagc gccgcgggct cagctcgacc ccgaccgcgt cgagcccgac cgcgttggcg
      961 accgcgagca cggtgccccg cccacaaaag ggatcgacga ccacgcggct cggcgtgcgc
     1021 cggcggatga actcgcaggc gaccacgcag gcctgcacgc ccatcgcccg cgaccaggtc
     1081 atcgccccgg tgtcgaacac gtcggccgcg ccgtcgggct cgcgcaatgg ttcacgcccg
     1141 aagcacagca ggtgggcgta cgcgggtctc ccgaaggtga tcgtgcccgc cggcttgcga
     1201 cacgcgatct tgtgccacca cagcgacgcc ccgcaattgt cgcctgcgat ctgcacgagg
     1261 tggcccttgt cgacccacac gcccgcgtgc ttgatgtccg tctgataaaa aatgaccgcg
     1321 ccgtccggcg ggcacgcgcg cagcaccgcc tcggccgcgg cgatgaacca cgcgcgccag
     1381 gccggcagcg tcatcgccag ctcggacacg tccggcagcg aggtgaccag cgaacacccg
     1441 gccagctcgc cccgcgccgc cagccaggcg agcgcgtcac cctcgtggat cgtccgcgcc
     1501 gcggtgcgct gttccgggtc gttcaaggcg gcgatggtag cgaggccccg cgtcgcgtca
     1561 atccagtc
//
LOCUS       DASWNJ010000326         2795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_288888, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000326 DASWNJ010000000
VERSION     DASWNJ010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288888"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2795)
                     /locus_tag="VGB85_05730"
     CDS             complement(<1..>2795)
                     /locus_tag="VGB85_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HEY0133555.1"
                     /translation="GNDGSDKIGFTAPSARGQATTLARAHAAAGVEVESIGYVESHGT
                     ATRLGDPIEIAALREVFEARTTARQFCALGAVKSNLGHLGAAAGVTGLIKATLALERQ
                     QIPGTLHFEAPAPALGLADSPFFVHSQPLPWPATTSPRRAGVSAFGVGGTSAHVILEE
                     SPVVAQSGPSRPWQALCLSARDAAALADRSRQLAASLRERPALALADVAHTLHRRPGF
                     GPERQVVVGRDHADAIAGLESGGTRVVRGNVRRDPPKLVFVFPGGGTQHAGMGRALYA
                     DEPVFRAHLDACAERFTAAIGEDIRGLLSPAAGLEEAAAQRLLTPSRNMATIFAVEVA
                     LGQLLISWGMLPAAVIGHSLGEYAAALFAGVMTLDDAVALVATRGLLCDESPPAAMVS
                     VSLSATDASRWLGPGLSLAAVNAADTCLISGPQEPMARLLEDLRRGEIEARVVPFGGA
                     AHSALMDPLVPRLLARVRGVQLHSPRLPMISCVTGMRVADEVCSPEYWGRHLRETVRF
                     HDALTTALADPEHVFIEVGPGGHLSGLIRRHAAAGPARLAVSAMQQPRTGRAEREALL
                     LAVAQLWCCGVEVAWSAMHGGEQRRPVQLPTYPFARTQHLLPPAASRRAAPAIVEAAP
                     PRRSAADSSEAPLPEPARTLAEIWAQLLGVPTVREGDNFYDLGGSSLVAIQVRARVLD
                     RLGVTIPTHALVDFPTLGGLAAQLDTYPRADARPAAAGFRENLLVRLKSGHPGVQPLF
                     LVQPLGGTVYTYLPLSRQLSWPGSIYGIRASGMEPGEPVFDDIHVIARRYLEEVTRVQ
                     PEGPVLLGGHSAGGIIAHEMAQQLLARGRSVSLVMLDAPSLPALREQAITDLDDLLRE
                     LTFFEAPESAAYQGLVTALCDGLALGPIMLATWRAIRGYEPRPLDCEVTYVTASEQRD
                     PRDTRACLYWMDLTVGD"
BASE COUNT          411 a          991 c         1057 g          336 t
ORIGIN      
        1 tagtccccga cggtcaggtc catccagtag aggcaggccc gggtgtcacg agggtcgcgc
       61 tgctcgctcg ccgtcacata ggtgacctcg cagtcgagcg gacgtggctc gtacccgcgg
      121 atcgcccgcc aggtcgcgag catgatcggg ccgagggcga ggccgtcgca cagggcggtg
      181 acgaggccct ggtaggcggc ggactcgggg gcctcgaaga acgtcagctc gcgcagcagg
      241 tcgtcgaggt ccgtgatcgc ctgctcgcgc agcgccggca gcgacggggc gtcgagcatc
      301 accaggctga cgctgcggcc gcgcgcgagc aactgctggg ccatctcgtg ggcgatgatg
      361 ccgccggcag agtggccgcc gagcagcacg gggccctcgg gctgcacgcg cgtgacctcc
      421 tcgaggtagc ggcgcgcgat gacgtggata tcgtcgaaca ccggctcgcc cggctccatc
      481 ccggaggcgc ggatcccgta gatactgccg ggccaggaca gctggcgcga cagcggtaga
      541 tatgtataca ccgtgccgcc gagcggctgc acgaggaaca gcggctgcac gccgggatgg
      601 ccggacttga ggcgtacgag caggttctcg cggaacccgg ccgcggccgg gcgagcgtcg
      661 gcgcgcgggt acgtgtcgag ctgcgcggcc agaccgccga gcgtcggaaa gtcgacgagg
      721 gcgtgcgtcg ggatcgtgac accgagacga tcgagcacgc gggcgcggac ctggatcgcg
      781 acgagcgagg agccgccgag atcgtagaaa ttgtcgccct cgcgcaccgt cggcacgccc
      841 agcagttgag cccagatctc ggccagcgtg cgcgccggct cgggcagcgg cgcctcgctg
      901 ctgtccgcgg ccgatcggcg cggaggcgcg gcctcgacga tcgcaggggc ggcgcggcgc
      961 gaggccgccg gcggtaacag gtgttgcgtg cgcgcgaagg gatatgtggg cagttgcacc
     1021 ggacgccgct gctcgccgcc atgcatcgcc gaccacgcga cctcgacccc gcagcaccac
     1081 agctgggcca cggcgagcag cagcgcctcg cgctccgccc tgcccgtgcg cggctgctgc
     1141 atcgcggaca cggccaggcg cgcgggccct gccgcggcgt gacggcggat caggcccgag
     1201 aggtggccgc cggggccgac ctcgatgaac acgtgctcgg ggtcggcgag cgcggtcgtc
     1261 agcgcgtcgt gaaagcggac cgtctcgcgc aggtgccggc cccagtactc ggggctgcaa
     1321 acctcgtccg cgacgcgcat cccggtcacg cacgagatca tcggcagccg cggtgaatgc
     1381 agctgcacgc cgcgcacgcg cgcgagcaag cgtggcacga gcgggtccat cagcgccgag
     1441 tgagcggccc cgccgaaggg gaccacgcgc gcctcgatct cgccgcgtcg caggtcctcc
     1501 aggagccgcg ccatcggctc ctgcgggcct gagatcaggc aggtgtcggc ggcgttgacg
     1561 gccgcgagcg aaagtccggg gccgagccag cgcgacgcgt cggtcgccga cagcgacacg
     1621 ctgaccatcg cggccggcgg gctctcgtcg cacagcaggc cgcgcgtggc gaccagcgcg
     1681 acggcgtcgt cgagggtcat gacgccggcg aacagcgcgg ccgcatattc gccgaggctg
     1741 tggccgatca ccgccgcggg cagcatcccc cacgagatca acaattgtcc gagcgcgacc
     1801 tcgacggcga agatcgtcgc catgttgcgc gagggcgtca gcaggcgctg cgcggcggct
     1861 tcctcgaggc cggccgcggg tgacagtagt ccacgaatgt cctcaccgat cgcggcggtg
     1921 aagcgctcgg cgcaggcgtc gaggtgcgcg cgaaacaccg gctcgtcggc atacagggcg
     1981 cgccccatgc ccgcgtgctg cgtaccgccg ccggggaaca cgaacaccag cttcggtgga
     2041 tccctgcgca cattgccgcg gacgacccgc gtgccgccgg actcgaggcc ggcgatcgcg
     2101 tcggcgtgat cgcggccgac gacgacctga cgctcgggac caaagccggg ccggcgatgc
     2161 aatgtatgtg cgacgtccgc gagggccagc gccgggcgct cgcgcaggct cgccgcgagc
     2221 tgccgcgagc gatccgccag cgccgcggcg tcgcgcgccg acaggcacag cgcctgccac
     2281 gggcgcgacg ggcccgactg cgcgacgacc gggctctcct cgaggatgac gtgcgcgctg
     2341 gtaccaccga ccccgaacgc gctcacaccg gcgcgccgcg gcgacgtggt cgcgggccac
     2401 gggagcggct gcgagtgcac gaagaacggg ctgtccgcga ggccgagggc cggcgccggt
     2461 gcctcgaagt gcagggtccc ggggatctgc tggcgctcga gcgcgagcgt ggccttgatc
     2521 agcccggtga cgcccgcggc ggccccgagg tgcccgaggt tgctcttgac cgccccgagc
     2581 gcacaaaact ggcgagcggt ggtgcgggcc tcgaacacct cgcgcagcgc tgcgatctcg
     2641 atcgggtcgc cgaggcgcgt cgcggtgccg tgcgactcga catagccgat gctctcgacc
     2701 tcgaccccgg cggccgcgtg ggctcgcgcg agcgtcgtcg cctgcccgcg agcgctcggg
     2761 gccgtgaacc cgatcttgtc cgaaccgtcg ttgcc
//
LOCUS       DASWNJ010000327         3843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_147123, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000327 DASWNJ010000000
VERSION     DASWNJ010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147123"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VGB85_05735"
     CDS             <1..508
                     /locus_tag="VGB85_05735"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEY0133556.1"
                     /translation="RTALEATGGDLVQSLVRVNSVIYKNVSERMADGRNLTLALLRYH
                     AGRVQLAGQHESVIVVRADGSVEEIDTLPLGINVGLLGEVDDMMSEMSFAFLPGDLMV
                     LYTDGVTEAERPDKQQYGVSRLKDSVTSRRALPCDQIVQGVFTDIYRWIGGGTVYDDI
                     TLVLVRRT"
     gene            532..1104
                     /locus_tag="VGB85_05740"
     CDS             532..1104
                     /locus_tag="VGB85_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133557.1"
                     /translation="MEHVYGDYDFVPAALVPEAQLALTLSPSELTAHWQRCGLISDFV
                     ASYVSTAYAAGDREASAPLFTAISTVFQELIENAAKFSRKREAPISVRVKHYSRILML
                     EVENGTTAAFAQRFEEYLTGMQASGDLDALYLRILESKLEDAAAAQRSGIGLLVLRKD
                     HGIRVGVRFAEDEQGRPTITVRAFQTLEGV"
     gene            1109..1453
                     /locus_tag="VGB85_05745"
     CDS             1109..1453
                     /locus_tag="VGB85_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133558.1"
                     /translation="MEVRETEAVVRFAPETGTVFFSGALRLANVKAYDKVVALLHDAA
                     KQAEGPLVLDFKELKFLNSSGITAISLFVVGARKAGTPQLRLVGAKTTAWQQKSLANF
                     QMLWKDMAVVIE"
     gene            1446..2978
                     /locus_tag="VGB85_05750"
     CDS             1446..2978
                     /locus_tag="VGB85_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133559.1"
                     /translation="MSDAARACCCGLALAIAGCQGEPSADEAPTGTGSTSEAPEPTGS
                     EGTGGDSSSGDGESSGDASSSGEPDEPVDCDDEDRVYAPPPVMVDGELAVPIDVLSLA
                     GEFTFDFAAQTTRARAELRFRVGPVEGRPIFDLRQDGAIVGSLDGAAIDGSGMPQRDL
                     GGGPGSEMRVLATALPACSEHLLVLEYPIEVLVGYAAPPPEFKEDGVAWDFGFGDLTP
                     RTFLEQWLPANLIHDRHPITLGVTIDGGPADQRVISNGPVVELAPGRWQVEFPGSSTA
                     QSPMLVLIPGARVESSSTTVTLPDGEYGIELHRRIEVDVSSAALHDSLAQSFAAFTAS
                     TGGYAFSGFTAVIDFNGGMEYDGGTVSSVEALPHELFHSWYGRGIRPARASDGWFDEA
                     WNEYNTAFPLFPAEPIAADAPPAVLCSDNPWSRTTPFTAYDEGRAVFAAIADAAGVAP
                     LRASMQAFYAAHVLEPVSTQELERHLHCSLDVPAVRELFHRRVYGKQGAAPAVPDGYC
                     EP"
     gene            3024..3821
                     /locus_tag="VGB85_05755"
     CDS             3024..3821
                     /locus_tag="VGB85_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133560.1"
                     /translation="MITVSVVLATACSTGEDGGASATESVTAATMPQTTGASTTNTPT
                     TGGVGSGVTTGSSTTGESTGAVSGTTGATGSSDDGGEPRLDLGVDSEGAGCAPEDTCC
                     QAEGFVPPHKLLDTFLAAYPAASMPKTDAELLKFEPVADGHAMAYSMDNVGNEFIDPD
                     KGGVTEANIMTGRMYSRTKAEAEVPVGATVLEVREDPIVIEDLGAGGNGKPGCIGVGW
                     GWGSIIFAAQDKSIGELVYLYVGYCHDGDAEAFYFSEQAVELCPAPG"
BASE COUNT          600 a         1205 c         1466 g          572 t
ORIGIN      
        1 gcgcacggcg ctcgaggcga ccgggggcga cctcgtgcag tcgctggtgc gcgtcaactc
       61 ggtcatctac aagaacgtca gcgagcgcat ggccgacgga cgcaacctga ccctggcgct
      121 gctgcgctat catgcgggcc gggtgcagct ggcgggacag cacgagagcg tgatcgtggt
      181 gcgcgcggat gggagcgtcg aggagatcga cacgcttccg ctcgggatca acgtcgggct
      241 gctcggcgag gtcgacgaca tgatgtcgga gatgagcttc gcgtttttgc ccggcgacct
      301 gatggtgctc tacaccgacg gcgtgaccga ggccgagcgg cccgacaagc agcagtacgg
      361 cgtttcgcgg ctcaaggact cggtgacgag ccggcgcgcg ctgccctgcg accagatcgt
      421 tcaaggcgtg ttcacggaca tctaccgctg gatcggcggc ggcaccgtgt acgatgacat
      481 caccctggtc ctggtgcgca ggacctgaga tcgaagcgaa gcgaggcggc catggaacat
      541 gtctacggcg attacgactt cgtccccgcg gcgctggtgc ccgaggccca gctcgcgctc
      601 acgctgtcgc ccagcgagct gacggcccac tggcagcggt gcgggctgat ctccgacttc
      661 gtggcgagct acgtgtcgac ggcgtacgcg gcgggcgacc gcgaggcgag cgcgccgctg
      721 ttcacggcga tctcgacggt gtttcaggag ctgatcgaga acgcggccaa gttctcgcgc
      781 aagcgcgagg cgccgatctc ggtgcgcgtg aagcactaca gccggatcct gatgctcgag
      841 gtcgagaacg ggacgacggc ggcgttcgcc cagcgcttcg aggagtacct gaccgggatg
      901 caggcgagcg gggacctcga cgcgctgtac ctgcggatcc tcgagagcaa gctcgaggac
      961 gccgcggccg cgcagcgctc ggggatcggc ctgctggtgc tgcgcaagga ccacgggatc
     1021 cgcgtcgggg tccgcttcgc cgaggacgaa cagggccgcc cgacgatcac cgtccgcgcg
     1081 ttccagactc tggaaggagt ttaagacaat ggaagtacgt gagacagaag ccgtggtccg
     1141 gttcgcgccg gagacaggga ccgtattttt cagcggcgcg ctgcgcctcg ccaacgtcaa
     1201 ggcctatgac aaggtcgtcg cgctgctgca cgacgcggcg aagcaggccg agggcccgct
     1261 ggtgctcgac ttcaaggagc tcaagttcct caacagctcg gggatcacgg cgatcagcct
     1321 gttcgtggtc ggcgcgcgca aggccggtac gccgcagctg cggctcgtcg gcgccaagac
     1381 gacggcgtgg cagcagaagt cgctcgcgaa ctttcagatg ctgtggaagg acatggccgt
     1441 cgtcattgag tgacgcggcg cgggcgtgct gctgcgggct ggccctggcg atcgccgggt
     1501 gccagggcga gccgtcggcc gacgaggcgc cgacggggac cggttcgacg agcgaggcgc
     1561 cggagccgac cgggagcgag ggcaccggcg gtgacagcag cagtggtgac ggcgagagca
     1621 gcggtgacgc gtcgagcagt ggtgagccgg acgagccggt cgactgcgac gacgaggatc
     1681 gggtgtatgc gccgccgccg gtgatggttg acggcgagct cgcggtgccg atcgacgtgc
     1741 tctcgctcgc cggagaattc acgttcgact tcgcggcgca gacgacccgg gcgcgcgccg
     1801 agctgcggtt tcgcgtcggg cccgtcgagg gccggccgat cttcgatctg cgacaggacg
     1861 gcgcgatcgt cggcagcctc gacggcgccg cgatcgacgg gtcgggcatg ccgcagcgcg
     1921 acctcggcgg cgggcccggc agcgagatgc gcgtattggc gaccgcgctg ccggcctgct
     1981 cggagcacct gctcgtgctc gagtatccga tcgaggtgct ggtcggttac gccgcgccgc
     2041 cgccggagtt caaggaggac ggcgtcgcgt gggacttcgg cttcggcgac ctgacgccgc
     2101 ggaccttcct cgagcaatgg ttgccggcca atctgatcca cgaccggcat ccgatcacgc
     2161 tcggcgtaac gatcgacggt gggccggccg atcagcgggt gatcagcaac gggccggtcg
     2221 tcgagctcgc gccggggcgc tggcaggtcg agtttccggg gtcgtcgacg gcgcagtcgc
     2281 cgatgctggt gttgatcccc ggggcgcgcg tcgagtcgag cagcacgacg gtcacgctgc
     2341 cggacgggga gtatgggatc gagctgcacc ggcggatcga ggtcgatgtc agctcggcgg
     2401 cgctgcacga ttcgctcgcg cagtcgttcg cggcgttcac ggcgtcgacc ggcggttacg
     2461 cgttttcggg cttcacggcg gtgatcgact tcaacggcgg gatggagtac gacgggggca
     2521 ccgtcagctc ggtcgaggcg ctgccgcacg agctgtttca tagctggtac gggcgcggga
     2581 tccggccggc gcgcgcgagc gacgggtggt tcgacgaggc gtggaacgag tacaacaccg
     2641 ccttcccgct gttcccggcc gagccgatcg ccgccgacgc gccgcctgcg gtgctgtgca
     2701 gcgataaccc gtggtcgcgc acgacgccgt tcaccgcgta cgacgagggc agggcggtgt
     2761 tcgcagcgat cgcggacgcg gccggtgtcg cgccgctgcg cgccagcatg caggcgtttt
     2821 atgcggcgca cgtgctcgag ccggtgagca cgcaggagct cgagcggcat ttacattgtt
     2881 cactcgacgt gcccgcggtg cgcgagctgt ttcatcgccg cgtgtacggc aagcagggcg
     2941 ccgcgcccgc ggtgcccgat ggttactgcg agccctgagt gagtggtagc gtgggccgtg
     3001 gaggtgcggt gatgcggcga gtgatgatca cggtgagcgt tgtgctggcg acggcgtgca
     3061 gcacgggtga agatggtggg gccagcgcga ccgagagcgt caccgcggcc acgatgccgc
     3121 agacgacggg cgcctcgacg accaacacgc cgacgaccgg cggcgtgggc tccggggtga
     3181 cgaccggatc gtcgaccacg ggtgagagca cgggcgcggt cagcgggacg accggcgcga
     3241 ccgggagcag tgacgacggc ggcgagccca ggctcgacct cggggtcgac agcgagggag
     3301 caggctgtgc gccggaggac acgtgctgtc aggcggaggg cttcgtgccg ccgcacaagc
     3361 tgctcgacac cttcctcgcg gcgtatccgg cggcgagcat gcccaagacc gacgcggagc
     3421 tgctcaagtt cgagccggtc gccgacggtc acgcgatggc gtactcgatg gacaacgtcg
     3481 gcaacgagtt catcgacccc gacaagggcg gtgtgaccga ggcgaacatc atgacgggtc
     3541 gcatgtactc gcgcacgaag gccgaggccg aggtcccggt cggggcgacg gtgctcgagg
     3601 tccgcgagga cccgatcgtg atcgaggacc tcggcgcggg cggcaacggc aagccgggct
     3661 gcatcggcgt cggctggggc tgggggtcga tcatcttcgc ggcgcaggac aagtcgatcg
     3721 gcgagctcgt gtatctgtac gtcggctact gccacgacgg cgacgcggag gcgttctact
     3781 tctccgagca ggcggtcgag ctgtgcccgg cgccgggctg agctctcacc ccggcggcag
     3841 cgc
//
LOCUS       DASWNJ010000328         2786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_253988, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000328 DASWNJ010000000
VERSION     DASWNJ010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253988"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VGB85_05760"
     CDS             <1..309
                     /locus_tag="VGB85_05760"
                     /inference="COORDINATES: protein motif:HMM:NF027798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,3-glucanase family protein"
                     /protein_id="HEY0133561.1"
                     /translation="VYACSGYFDSQPPNCMPATKDCHFEGNKWCAALNRGMLNDPESV
                     DTSEYYQSTPHNTYSKWVHATCPGIYAFAYDDYPASGGESGFRACKAPRLDITFCPAG
                     "
     gene            complement(343..645)
                     /locus_tag="VGB85_05765"
     CDS             complement(343..645)
                     /locus_tag="VGB85_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEY0133562.1"
                     /translation="MSRRPNETEQEYQARLAMERQREAAQQRHSALGDDDRERQRHLH
                     WMHCPKCGAELAEVQFRDVKLDKCFACGGVYLDDGELEQLTGKPGWFESMLRFFHN"
     gene            complement(674..>2786)
                     /locus_tag="VGB85_05770"
     CDS             complement(674..>2786)
                     /locus_tag="VGB85_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133563.1"
                     /translation="DPRSFPSVTAEPRRPAAASMLEAVTKAVAAGEERRHAADVPSAD
                     PTRGRSQPILIADLARAAAASAEKAVQKVDGQRARERAERSTGTFTGAMSRVDPSRTD
                     PVRSESSMLNHRDGRSSTRPITGSHATIGSAGTIGTSAGRPSTIPPRDSPIPPREGRR
                     ETADLKLATAAARAAELRMVAAREGEPTQVSHPPPGASFDDSTGGETAEIPRPPEVVI
                     VKVQETSGRGGSAALWGLLGVAAAAGIVFGVPSIRERVWNLGGPPPVTRADTDQPAVA
                     PLEDTTPAEVEQARKAIRNLGFKESNKVQAALQRVIDDPKRSPQAMTQARLTQAELVL
                     MRALACKIAVIIEPAAMGGQAQTRSVEDPVYAEELLASIGELPAPDPADPDAAAKAAV
                     ADQLRRVRALQALVEGKPAGELPPGSEELAALIKGAPLWRNEVRTPPAGLITSLQRLA
                     NPSSLSQSLLALALWRSGDVDGSRALLRTILDRVADQPAARTMMEALDRQDMLAENGD
                     PELAVPPSDPESEPEMPPEPADPTAGGETPTPTTPAIPGDGKTGPVRTTPGVAQERVE
                     VLISTGCQKVRQGDPMGVKLLLDAVDRGADPMQNFNLCFCLGAGFARQNSHDAALTWY
                     ARAVRQSPQNRDAIAGAARSAELLGRTGSAVDYYKKLRALDPGNAAAAAYLSKHDQSH
                     EPPPPSDDPPGELMPIKPKQR"
BASE COUNT          330 a         1020 c         1011 g          425 t
ORIGIN      
        1 gtctacgcct gctccggcta cttcgacagc cagccgccca actgcatgcc ggccacgaag
       61 gactgccact tcgagggcaa caagtggtgc gccgcgctca atcgcggcat gttgaacgac
      121 cccgagtcgg tcgacaccag cgagtactat cagtccacgc cccacaacac ctattcaaag
      181 tgggtgcacg ccacctgccc cggcatctac gcgttcgcct acgacgatta cccggccagc
      241 ggcggcgaga gtggctttcg cgcctgcaag gcgccgcgcc tcgacatcac cttttgcccc
      301 gcagggtgaa acggatgagg cgacgatcga cggcgctcgc cgtcagttat gaaaaaaccg
      361 cagcatgctc tcgaaccacc ccggcttgcc ggtcagctgc tcgagctcgc cgtcatcgag
      421 gtaaacgccg ccgcaggcga agcacttgtc gagcttgacg tcgcggaact ggacctcggc
      481 cagctcggcg ccgcacttgg ggcagtgcat ccagtgcagg tggcgctggc gctcgcggtc
      541 gtcgtcgccg agggcagaat gccgctgctg ggcggcctca cgctggcgct ccatcgcgag
      601 gcgggcctgg tactcctgct ctgtctcgtt gggtcggcga ctcatggcac cctccgtcct
      661 ttcgtgcccc gggctagcgc tgcttgggct tgatcggcat cagctcgccc ggtgggtcat
      721 cgctgggcgg cggcggctca tggctctggt cgtgcttcga caggtaggcg gccgcggccg
      781 cgttgcccgg gtcgagcgcc cgcagcttct tgtaataatc caccgccgag ccggtgcgcc
      841 cgagcagctc ggccgagcgc gccgcgcctg cgatcgcgtc gcggttttgc ggcgactggc
      901 ggaccgctcg cgcgtaccag gtgagcgccg cgtcgtggct gttctgccgg gcgaagcccg
      961 ccccgaggca aaagcagagg ttgaagttct gcatcgggtc ggcgccgcgg tcgacggcgt
     1021 cgagcagcag cttgacgccc atcgggtcgc cctggcgcac cttctggcag ccggtcgaga
     1081 tcaggacctc gacgcgctcc tgggcgacgc ccggggtcgt gcgcaccggg ccggtcttgc
     1141 cgtcgccggg gatcgccggg gtggtcggcg tcggcgtctc gccgccggcc gtcggatccg
     1201 ccggctcggg gggcatctct ggctcggact ccggatccga gggcggcacc gcgagctcgg
     1261 ggtcgccgtt ttcggcgagc atgtcctggc ggtcgagcgc ctccatcatc gtgcgcgcgg
     1321 ccggctgatc ggcgacgcgg tcgaggatcg ttcgcagcag cgcgcgggag ccgtcgacgt
     1381 cgccgctgcg ccacagcgcc agcgcgagca gcgattgact gaggctcgac gggttggcga
     1441 ggcgctgcag cgaggtgatc aggccggccg gcggcgtgcg gacctcgttg cgccacagcg
     1501 gcgcgccctt gatcagcgcc gcgagctcct cgctgccggg cggcagctca ccggccggct
     1561 tgccctcgac gagcgcctgc agggcccgca cgcgacgcag ctggtccgcc accgcggcct
     1621 tggccgccgc gtccgggtcc gcggggtcgg gcgccggcaa ctcgccgatg ctcgccagca
     1681 gctcctcggc gtagaccggg tcctcgacgc tgcgggtctg ggcctgtccg cccatcgcgg
     1741 ccggctcgat gatgacggcg atcttgcagg cgagcgcgcg catcaacacc agctcggcct
     1801 gcgtcagccg ggcctgcgtc atcgcctgcg gcgagcgctt ggggtcgtcg atgacgcgct
     1861 gcagcgccgc ctggaccttg ttgctctcct tgaagccgag gttgcggatc gccttgcggg
     1921 cctgctcgac ctccgccggc gtcgtgtcct cgagcggggc gaccgcgggc tggtcggtgt
     1981 cggcgcgggt caccggcggc ggaccgccga ggttccacac gcgctcgcgg atgctcggca
     2041 ccccgaacac gatgcccgcc gccgccgcga ccccgagcag gccccacagc gccgccgagc
     2101 cgccgcgccc gctggtctcc tgcaccttga cgatgacgac ctcgggcggc cgcgggatct
     2161 cggccgtctc gccgccggtc gagtcgtcga acgaagcgcc cggtggcggg tggctgacct
     2221 gggtcggctc gccctcgcgg gccgccacca tgcgcagctc ggccgcccgc gcggccgcgg
     2281 tcgccagctt gaggtccgcg gtctcgcgcc ggccctcacg cggagggatc ggcgagtcgc
     2341 gcggcgggat cgtgctcggg cggccagcag acgtgccgat cgttccagcc gagccgatcg
     2401 tcgcgtggct gccggtgatc ggccgcgtgc tgctgcggcc gtcgcggtga ttgagcatgc
     2461 tcgactcgga gcgcaccggg tcggtgcgcg acgggtcgac gcgagacatc gcgccggtga
     2521 aggtgccggt cgaccgctcc gcccgctcgc gggctcgctg gccatcgacc ttctgcacgg
     2581 ccttctcggc gctcgcggcg gccgcccgcg ccaggtcggc gatgaggatc ggctggctgc
     2641 gaccgcgggt cgggtccgcc gacgggacgt ccgcggcgtg ccggcgctcc tcgcccgccg
     2701 cgaccgcctt ggtcaccgcc tcgagcatcg aggccgccgc cgggcgccgc ggctcggccg
     2761 tgacgctcgg aaaactgcgc gggtcc
//
LOCUS       DASWNJ010000329         7123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_537018, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000329 DASWNJ010000000
VERSION     DASWNJ010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7123
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537018"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2915
                     /locus_tag="VGB85_05775"
     CDS             <1..2915
                     /locus_tag="VGB85_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133564.1"
                     /translation="LPAREASVVDLDGDRRSDLVLLDGSRRVVTLVDHDLRVLRSDRM
                     PAAVSHLIPGHAQALVHDGQSLQLHALGGPPTLLWQQASASKPYTAAAIDLADDGQPD
                     LFLGFEAPERRFAVLPDAAVAPPGALLRPAHPATQASESDIEGLAAADLDGDGTRELI
                     AGVGAWTAYDLRVFQAGPEGLVLRAYRRMGRIGGVAVVRDAGGRARIAVARDGMYPNR
                     EIFPTPPHTGAPAGVYLLRWTGDALVEDGFVPFPRVDGASALELGRVMLSGDLDGDGL
                     DDLVLAATRQSTRAQEQRRALTLVLRQRIDGDFALVALGGVAPVALFDADDDPARELL
                     AVATERADELWILGAGTEIMDPLPRPSLTPVEAPAGLADPALVRAWQRASQLERVGLA
                     REAATTLRDAAHFAGDPHDRRLVLDRAGDILLAHGDEAGAGDLFRQVAADPALAPAAL
                     AKAAEAATRLGRYADAHAALQALIGLRAATPAQIEAARGDIAELEPMARPEHSFRVDF
                     DRPLGPAWHIEDPSAVRRDPGDGVLRVTTFPGQHELAALPIAWDGGPLRVHVDLVREH
                     LELGASLRVVVRGDDGSLWLGAGVAASGGGGLVEQALQCWNRHHGVWDAIDGRPQASA
                     EEHEALRLRIEYLPGMGPLTCGVTDGRGLVHRFQGPPAAPAPGGRYWLSIGVFPDRPI
                     DLAMSRAAIRSIQVEGAQALPPVPESLAAAARRRLAEGEPLSALAQWAEQDTAPTGPP
                     DPRRHLWEAVAHDDLHHGPELVAALRRALPLDPAEPLDPILLRLLHLRPASFGAALRE
                     LLGPRFYAVYDAAWGTTIHHHRYDPFLHRQLTADLADLDAFAPQSDAEWALKFRLLAL
                     RGRSWLRLGVTERAREDLRAAAAMLPRVPPGERAHIVALVWILAAKVTAGEDPTEALG
                     YAQVAVATSPTPELVAEQLRSDPDLGAFQGDPAWAGVFAPASPPTPSPAPSPGSPP"
     gene            2950..4203
                     /locus_tag="VGB85_05780"
     CDS             2950..4203
                     /locus_tag="VGB85_05780"
                     /inference="COORDINATES: protein motif:HMM:NF005744.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY0133565.1"
                     /translation="MTTTPTLSRRGATAPASPIRRLTPYADAARARGIRVHHLNIGQP
                     DLHTVSPMRAAYARFADEVIAYSPSEGYLYYRQALAGYYNGLGHDAGGGPIDPSQILV
                     TVGGSEALLFTIAAACDPGDEILVAEPYYTNYAGFSHMLGVTVTPVSTHASESFAIPV
                     DRVRAAITPKTRAFIVPSPGNPTGMVLSSDELRALGQVCVEANIFFVVDEVYREFVYP
                     EPPVPSDRPLPPAPSVLAIPELAQHAVMIDSVSKRYSACGARVGCLVTRNKSFYAACL
                     KFAQARLSPPTVDQFAAAAALDTPLPEMRAMIDAYRARRDLIVAGLNRIPGVSCPTPT
                     GAFYLVTDLPVADAEDFCIFMLRDFDLDGETVMMAPAEGFYATPGMGRNQVRLAYVLE
                     PSKLTRCLEILRAGLEAYARKHAAT"
     gene            4257..5075
                     /locus_tag="VGB85_05785"
     CDS             4257..5075
                     /locus_tag="VGB85_05785"
                     /inference="COORDINATES: protein motif:HMM:NF025124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0133566.1"
                     /translation="MSLVAPTPLGFERSPVSGTSLDDLDLAALHAFVRRRIAGLPAGD
                     PPDAAVQTTLDRGAQRLGLVGTISSQQVPTVVGLMAFGTAPQLARPEWGVSCVRVRGA
                     NLADEVAARLDVEGPLAALVDQTLDFVRSNTRVAPNLLRPSEPASEYPEVAVREALLN
                     ALLHRDYRLSGRVSLRIFNDRLEVWSPGGLALPLSLDELAQHGGASFPRNPLLMATAR
                     SLGLVEQVGRGIPLIRKTLGDATSLPVQFSAAQTEVRVVLPSYINASMGNVAGN"
     gene            complement(5392..>7123)
                     /gene="ftsH"
                     /locus_tag="VGB85_05790"
     CDS             complement(5392..>7123)
                     /gene="ftsH"
                     /locus_tag="VGB85_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY0133567.1"
                     /translation="DITYEFDKKLYQATGPVAEGLYQKLSEKGIDYTVEPEEDGALLQ
                     FALMWLPMIFIVVLMVMFMRQMQAGGGRAMNFGKSKARLLNEHTNKVTFKDVAGVEEA
                     REDVEEIIDFLRDTRKYLRVGGRIPKGVLLMGPPGTGKTLLARAIAGEAGVAFFSISG
                     SDFVEMFVGVGASRVRDLFEQGKKNATCIIFIDEIDAVGRHRGTGLGGGHDEREQTLN
                     QLLVEMDGFEGNDGVILVAATNRPDVLDPALLRPGRFDRRIIVGLPDVRGREAILKVH
                     TRKVPVDDNVDLATVARGTPGFSGADLENLVNEAALMAARFGRDRVTEDDFDRAKDKV
                     MMGSERKSAVISDRERMTTAWHEAGHTLIAVSLPKEHVDPIHKVTIIPRGRALGVTQQ
                     LPEEDRYNLDREHVEAQIAILMAGRIAEEIAFNGQKTTGATNDIERATHLARKMICEW
                     GMSDVLGPISYTKAEGQPFIGREMQHAPTYSEATAQQIDRAIHEIVTRQYTLARKILT
                     EKQDALRRLAETLFEREVLTLSEVLSVIEGRALAPMRPRPRMAVTGQVVPGIDRPVST
                     PGKLVAEPST"
BASE COUNT          895 a         2825 c         2353 g         1050 t
ORIGIN      
        1 agctgcccgc gcgcgaggcc agcgtcgtcg acctcgacgg agatcgtcgc agcgacctcg
       61 tcctgctcga cggcagccgc cgcgtcgtca cgctcgttga tcacgacctg cgcgtcctgc
      121 gaagcgaccg catgccggcc gccgtctcgc acttgatccc cgggcacgcg caggcgctcg
      181 tgcacgacgg ccagagcttg cagttgcacg cgctcggcgg tccgccgacc ctcctctggc
      241 agcaggcgag cgcgagcaag ccgtacaccg ccgccgcgat cgacctcgcc gacgacggcc
      301 agcccgacct cttcctcggc ttcgaggcgc ccgagcggcg cttcgccgtg ctgccggacg
      361 ccgccgtcgc gccccccggc gccctgctgc gtcccgccca cccggcgacc caggccagcg
      421 agagcgacat cgagggcctc gccgccgccg acctcgacgg cgacggtacg cgcgagctga
      481 tcgccggcgt cggggcgtgg accgcctacg acctgcgcgt gttccaggcc ggccccgagg
      541 gcctggtcct gcgcgcctac cgacgtatgg gccggatcgg cggcgtggcg gtcgtgcgcg
      601 acgccggtgg tcgggcccgc atcgccgtcg ccagggacgg catgtatccc aaccgcgaga
      661 tcttcccgac gccgccgcac accggcgcgc ctgcgggcgt gtacctgctg cgctggaccg
      721 gcgacgcgct cgtcgaggac ggctttgtcc cgttcccgcg ggtcgacggc gccagcgcgc
      781 tcgagctcgg ccgcgtcatg ctctcgggtg acctcgacgg cgacggcctc gacgacctcg
      841 tgctcgccgc gacccgccag agcacgcgcg cgcaggagca gcggcgggcc ctgaccctcg
      901 tgctgcgcca gcgcatcgac ggcgacttcg ccctcgtcgc gctcggcggc gtcgccccgg
      961 tcgcgctgtt cgacgccgac gacgaccccg cgcgcgagct gcttgcggtc gccaccgaac
     1021 gcgccgacga gctgtggatc ctcggcgccg gcaccgagat catggacccg ctgccgcgcc
     1081 cgagcctgac gcccgtcgag gcgcccgccg ggctcgccga cccggccctc gtgcgcgcct
     1141 ggcagcgcgc cagccagctc gagcgcgtcg gcctcgcccg cgaggccgcg accaccctgc
     1201 gcgacgcggc tcacttcgcc ggcgacccgc acgaccgccg cctcgtgctc gaccgcgccg
     1261 gcgacatctt gctcgcgcac ggcgacgagg ccggtgccgg cgacctgttc cgccaggtcg
     1321 ccgccgatcc tgcgctcgcc ccggccgcgc tcgccaaggc cgccgaggcc gcgacccgcc
     1381 tcggccgcta cgccgacgcc cacgccgccc tgcaggcgct gatcggcctg cgcgcggcca
     1441 ccccggcgca gatcgaggcc gcccgcggcg acatcgccga gctcgagccg atggcccgac
     1501 ccgagcacag ctttcgcgtc gacttcgacc gcccgctcgg gcccgcgtgg cacatcgagg
     1561 acccgagcgc cgtgcgccgc gaccctggcg acggcgtcct gcgcgtcacc accttccccg
     1621 gccagcacga gctcgcagcc ctgccgatcg cgtgggacgg cggacccttg cgtgtacacg
     1681 tcgacctcgt gcgcgagcac ctcgagctcg gcgcctcgct gcgcgtcgtc gtgcgcggcg
     1741 acgacggcag tctctggctc ggcgccggcg tcgctgcctc cggcggcggc ggcctcgtcg
     1801 agcaggcgct gcagtgctgg aaccgccacc acggcgtgtg ggacgcgatc gacggccgcc
     1861 cgcaggccag cgccgaggag cacgaggcgc tgcgcctgcg catcgagtac ctgccgggca
     1921 tgggcccgct gacctgcggc gtcaccgatg gtcgcggcct cgtgcatcgc ttccagggtc
     1981 cacccgccgc gccggccccc ggcggccgct actggctctc gatcggcgtg ttccccgatc
     2041 gaccgatcga cctggcgatg agccgcgcgg cgatccgcag catccaggtc gagggcgcac
     2101 aggccctgcc gccagtgccg gagagcctcg ccgccgccgc gcgacgaagg ctcgccgagg
     2161 gcgaaccact ctcggcgctc gcgcagtggg ccgagcagga caccgccccc accggtccgc
     2221 ccgacccccg gcgtcatctc tgggaggccg tcgcccacga cgacctgcac cacggccccg
     2281 agctcgtcgc cgccctgcgc cgcgcgctgc ccctcgaccc cgcggagccg ctcgacccga
     2341 tcctgctgcg cctgctgcac ctgcgccccg ccagcttcgg cgccgccctg cgcgagctgc
     2401 tcggcccgcg cttttacgcc gtctacgacg ccgcgtgggg cacgacgatc caccaccacc
     2461 gctacgaccc cttcctgcac cgccagctga ccgccgacct cgccgacctc gacgccttcg
     2521 ccccgcagag cgacgccgag tgggcgctca agtttcgcct gctcgccttg cgcggccgaa
     2581 gttggttacg cctcggtgtt accgaacgcg cgcgcgagga cctgcgcgcc gccgcggcga
     2641 tgttgccgag ggtcccgccc ggcgagcggg cccacatcgt cgcgctggtg tggatcctcg
     2701 ccgccaaggt caccgccggc gaggacccga ccgaggccct cggctatgcc caggtcgccg
     2761 tcgccacctc cccgaccccc gagctcgtcg ccgaacagct gcgcagcgac cccgacctcg
     2821 gcgccttcca gggcgacccg gcgtgggccg gcgtgttcgc ccccgcgagc ccgccgacgc
     2881 cctcccccgc cccctccccc ggctcgcccc cttgagactt gccccgcttt gccgcaccct
     2941 cgcgctgcct tgaccaccac gccgaccctg tcccgccgcg gcgcgaccgc gcccgcctcg
     3001 ccgatccgcc gcctcacgcc ctacgccgac gccgcgcggg cccgtgggat ccgggtgcac
     3061 cacctcaaca tcggccagcc cgacctgcac accgtctcgc cgatgcgcgc cgcctatgcc
     3121 cgcttcgccg acgaggtgat cgcttacagc ccgtccgagg gctatctcta ctaccgccag
     3181 gcgctcgccg gttattacaa cggcctcggc cacgacgccg gagggggccc gatcgacccc
     3241 tcgcagatcc tcgtcaccgt cggcggctcc gaggccctgc tgttcacgat cgccgccgcc
     3301 tgtgatccgg gcgacgagat cctcgtcgcg gagccctact acaccaacta cgccggcttt
     3361 tcgcacatgc tcggggtgac cgtcacgccg gtctcgacgc acgcgagcga gtcgttcgcg
     3421 atccccgtcg accgcgtgcg cgccgcgatc acgccgaaga cgcgggcctt catcgtgccc
     3481 tcaccgggca acccgaccgg catggtgctc tcgtccgacg agctgcgcgc gctcggccag
     3541 gtctgcgtcg aggccaacat cttcttcgtc gtcgacgagg tctaccgcga gttcgtgtac
     3601 cccgagccgc ctgtcccttc agacaggccc ctgccgcccg cgccctccgt gctggcgatc
     3661 cccgagctcg cccagcacgc cgtgatgatc gactcggtct ccaagcgcta ctccgcctgc
     3721 ggcgcgcgcg tcggctgcct cgtcacccgc aacaagtcgt tttacgccgc ctgcctcaag
     3781 ttcgcgcagg cccgcctgtc gccgccgacc gtcgaccagt tcgccgccgc cgccgcgctc
     3841 gacaccccgc tgcccgagat gcgcgcgatg atcgacgcct accgggcccg ccgcgacctg
     3901 atcgtcgccg gtctcaatcg gatccccggc gtctcctgcc cgaccccgac cggcgccttc
     3961 tacctcgtca ccgacctccc ggtcgccgac gccgaggact tttgcatctt catgctccgc
     4021 gacttcgacc tcgacggcga gaccgtgatg atggcccccg ccgagggttt ttacgcgacc
     4081 cccggcatgg gccgtaacca ggtccgcctc gcgtacgtgc tcgagccgag caagctgacc
     4141 cgctgcctcg agatcctgcg cgccggcctc gaagcctacg ctcgcaagca cgccgcgacc
     4201 tgagccgccc ggtaccgcgg cccgccggcc caaagtcgcg gtaccatcgc ggctcgatga
     4261 gcctcgtcgc ccccaccccg ctcggcttcg aacgcagccc cgtctccggc accagcctcg
     4321 acgacctcga tctcgcggcc ctgcacgcct tcgtgcgccg gcggatcgct ggtctgcccg
     4381 ccggggatcc gcccgacgcc gccgtgcaga cgacgctcga ccgcggcgcg cagcgcctcg
     4441 gcctcgtcgg caccatcagc tcgcagcagg tcccgaccgt cgtcggcctg atggccttcg
     4501 gcaccgcccc gcagctcgcc cggcccgagt ggggcgtctc ctgcgtgcgc gtgcgcgggg
     4561 caaacctcgc cgacgaggtc gccgcgcgcc tcgacgtcga gggcccgctc gccgcgctcg
     4621 tcgatcagac cctcgacttc gtgcgcagca acacccgcgt cgcgccgaat ttgttacgcc
     4681 cgagcgagcc ggcctccgag tacccggagg tcgccgtgcg cgaggccctg ctcaacgccc
     4741 tgctgcaccg cgactatcgc ctgagcggcc gcgtctcgct gcggatcttc aacgaccgcc
     4801 tcgaggtgtg gagccccggc ggcctcgccc tgccgctctc gctcgacgag ctggcccagc
     4861 atggaggcgc ctcgttcccg cgcaacccgc tgctgatggc gacagcccgc tcgctgggcc
     4921 tcgtcgagca ggtcggccgc gggatcccgc tgatccgcaa gaccctcggc gacgcgacca
     4981 gcctgccggt gcagttctcc gcggcgcaga ccgaggtgcg ggtcgtgctg ccctcgtaca
     5041 tcaacgcgtc gatgggcaac gtcgccggca attaatagcc gccgtagcac ttgccatcgt
     5101 agcagtcgcc cggctcgcag acgccggcgc gcccatcgtc gtcgttggtc cgtcaccgtc
     5161 ctcgccgggg tcgccggtct cgtcgtcgcc gccggaaaag gcgctgctct tggtcgtctc
     5221 gccgtcgtcg ttcgagcacg cgaacgacaa catcaggagc gggacacacg ggatggcgag
     5281 cgccgggcgg aacatgacag ccgactctac cagcgacggc ggcgttcaca ttaaatgcga
     5341 agggccggcg cctcgacggc gaccggccct ctcctgtgcg tatcgctgct ttcaggtcga
     5401 cggctcggcg acgagcttgc cgggcgtgct gaccgggcgg tcgatgccgg ggaccacctg
     5461 accggtgacg gccatgcggg gacgcgggcg catcggcgcg agggcgcgac cctcgatgac
     5521 cgacaggacc tcggagaggg tcaggacctc gcgttcaaac aacgtctcgg ccagccgacg
     5581 cagggcgtcc tgcttctcgg tcaggatctt gcgggcgagc gtgtactggc gggtcacgat
     5641 ctcgtggatc gcacggtcga tctgctgggc ggtcgcctcc gagtaggtcg gggcgtgctg
     5701 catctcgcga ccgatgaagg gctggccctc ggccttggtg tacgagatag ggccgagcac
     5761 gtcgctcatg ccccactcgc agatcatctt gcgggcgagg tgggtcgcgc gctcgatgtc
     5821 gttggtggcg ccggtcgtct tttgcccgtt gaacgcgatc tcctccgcga tgcggccggc
     5881 catcaggatc gcgatctggg cctcgacgtg ctcgcggtcg aggttgtagc ggtcctcctc
     5941 gggcagctgc tgggtgacgc cgagggcccg gccgcgcggg atgatcgtca ccttgtggat
     6001 cgggtcgacg tgctccttcg gcaggctgac ggcgatcagc gtgtgtccgg cctcgtgcca
     6061 cgcggtcgtc atgcgctcgc ggtccgagat cacggcgctc ttgcgctccg agcccatcat
     6121 gaccttgtcc ttggcgcggt cgaagtcgtc ctcggtcacg cggtcgcggc cgaagcgggc
     6181 ggccatcagg gcggcctcgt tgacgaggtt ctcgaggtcc gcgccgctaa agcccggcgt
     6241 gccgcgggcg acggtcgcga ggtcgacgtt gtcgtcgacc gggaccttgc gcgtgtgcac
     6301 cttgaggatc gcctcgcggc cgcggacatc cgggaggccg acgatgatgc gacggtcgaa
     6361 gcgaccgggg cgcagcagcg ccgggtcgag cacgtcgggg cggttggtcg cggcgacgag
     6421 gatcacgccg tcgttgccct cgaagccgtc catctcgacg agcagctggt tgagggtctg
     6481 ctcgcgctcg tcgtgaccgc cgccgaggcc ggtgccgcgg tggcgaccga cggcgtcgat
     6541 ctcgtcgatg aagatgatgc aggtggcgtt ctttttgccc tgctcgaaca ggtcgcgcac
     6601 gcggctggca ccgacgccga cgaacatctc gacgaagtcc gagccgctga tgctaaagaa
     6661 cgccacgccc gcctcaccgg cgatcgcgcg ggcgagcagc gtcttgccgg tgcccggggg
     6721 gcccatcagc agcacgccct tggggatccg gccgccgacg cgcaggtact tccgggtgtc
     6781 gcgcaggaag tcgatgatct cctcgacgtc ctcgcgggcc tcctcgacgc cggcgacgtc
     6841 cttgaaggtg accttgttgg tgtgctcgtt gagcaggcgc gccttcgact tgccgaagtt
     6901 catcgcccgg ccgccgccgg cctgcatctg ccgcatgaac atcaccatca gcacgacgat
     6961 gaagatcatc ggcagccaca tcagcgcgaa ctgcagcagc gcgccgtcct cctcgggctc
     7021 gaccgtgtag tcgatgccct tctcgctgag cttctggtac aggccctcgg cgaccgggcc
     7081 ggtcgcctgg tagagcttct tgtcgaactc gtaggtgatg tcc
//
LOCUS       DASWNJ010000330         2626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_321942, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000330 DASWNJ010000000
VERSION     DASWNJ010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321942"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /gene="der"
                     /locus_tag="VGB85_05795"
     CDS             <1..1180
                     /gene="der"
                     /locus_tag="VGB85_05795"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEY0133568.1"
                     /translation="ENVGLMRHIRAQAEIAIEEADLILFVVDLVDGLSSDDREIAELL
                     RRSGRPLLVVANKADNPAREMAAADMHELGLGDVIAVSAAHGRNVGELNEAILARLAR
                     TSPAGSVVAVPEGTRVAFMGRPNAGKSTLINALLHAPRVIVDAEAGTTRDPIDLPFVY
                     NEQPIVLIDTAGLRRRKQIDKAMEKLAAIKAIRTMERTQVVVLVLDASTGVTDQDQRI
                     ARMAHERGKGVVVVLNKWDLVARDKKKAHEIWTETQDELAFLEKPFTIKTSVVGSGRD
                     LGQGHARGLDELIEACLHTARALGKRISTADLNDELMAAVAEHSPPMFGPKPVKLLFA
                     TQAESDPPLIVISANHGRCLSPAYERYLLRRFRTRWNLRGVPVRLVVRGRGKGNHERA
                     "
     gene            1524..>2626
                     /locus_tag="VGB85_05800"
     CDS             1524..>2626
                     /locus_tag="VGB85_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0133569.1"
                     /translation="MLLGACLDHPLKAVEYDKSEEAENTVAIAINKDVDILFVIDNSG
                     SMGEEQQLLSENFASFINVLEAKDVQANYRIGITTTDAGNPRCPTATYKPEGGNLVLS
                     SCLDRIEQNEFKFGEDDFSAACTSKCTKRDADLTVRGTTTAYDMNEVPRKWIERIETE
                     SNIQGVDSNVEAFQCYGPQGVAGCGFESHLESMYLALANATSSASKNNYGFLREAAIL
                     SIVFITDEADCSYNPLTKEIFTTNKVFWKSPDDPAPTSSLCWKAGVECTGGPGTYSEC
                     HSQNYDVTGAVTTDPTKMALQPVSKYIDFVKSIEAEKQLIDESQNVLVSLIAGVPKGY
                     EDLQAEIPYEDSADTMLQGNFGIGPGCVLDAGTA"
BASE COUNT          450 a          941 c          852 g          383 t
ORIGIN      
        1 cgagaacgtc gggctgatgc gccacatccg ggcccaggcc gagatcgcga tcgaggaggc
       61 cgacctgatc ctgttcgtcg tcgacctcgt cgacggtctg tcgagcgacg accgcgagat
      121 cgccgagctg ctgcgtcgct ccggtcgacc gctgctcgtc gtcgccaaca aggccgacaa
      181 cccggcgcgc gagatggccg cggcggacat gcacgagctc ggcctcgggg acgtcatcgc
      241 cgtctccgcg gcgcacggcc gcaacgtcgg cgagctcaac gaggcgatcc tggcccgtct
      301 ggcgcgcacg tccccggcgg gcagcgtcgt cgcggtgccc gagggcacgc gggtcgcgtt
      361 catgggtcgg cccaacgccg gcaagtcgac gctgatcaac gcgctgctgc acgcgccgcg
      421 cgtgatcgtc gacgccgagg ccggcacgac gcgcgacccg atcgacctgc cgttcgtcta
      481 caacgagcag ccgatcgtcc tgatcgacac cgcgggcctg cgccgccgca agcagatcga
      541 caaggcgatg gagaagctcg ccgcgatcaa ggcgatccgc acgatggagc gcacccaggt
      601 ggtcgtgctc gtgctcgacg ccagcaccgg cgtcaccgac caggaccagc gcatcgcccg
      661 gatggcgcac gagcgcggca agggcgtcgt cgtcgtgctc aacaagtggg atctcgtggc
      721 ccgcgacaag aagaaggcgc acgagatctg gaccgagacc caggacgagc tcgcgttcct
      781 cgagaagccc ttcacgatca agacctcggt ggtcggcagt ggacgggatc tcggccaggg
      841 ccacgcccgc ggcctcgacg agctgatcga ggcctgcctg cacaccgcgc gcgcgctcgg
      901 caagcggatc tccaccgccg acctcaacga cgagctgatg gccgccgtcg cggagcactc
      961 gccgccgatg ttcggcccca agccggtcaa gctgctgttc gccacccagg ccgagagcga
     1021 tcccccgctg atcgtcatct ccgccaacca cggccggtgt ttgtccccgg cctacgagcg
     1081 ctatcttctg cgccgcttcc ggacccgctg gaacctgcgc ggcgtgcccg tccgcctggt
     1141 cgtccgcggc cgcggcaagg gcaaccacga gcgcgcctga ggacgccggt gcgccaaacc
     1201 ggcgggtccc gcggcgtcct gtcacttcct ggcggctgcc tgcgggggcc tcgcccccct
     1261 gaaaagtgcg tccatgcgtc ggcatagcga gccggcacgg cgccggggac ggcggctcgg
     1321 tgaacggccg gccctcgccg tagcggccga tgggcgcggt cgcgcgggcg atgcagttgc
     1381 ctcgcggctg tcgtagtgct ttacacggcc cctgtccttt gcgatggtgg cgggcgtagg
     1441 gctcgccccg cggagaatcc acaatgtcgc gtactttttc gtcctcccgc cttgccaatc
     1501 tcacgctctg tgccggtacg gcgttgctgc taggcgcctg cctcgaccac ccgctcaagg
     1561 cggtcgagta cgacaagtcc gaggaggccg agaacacggt cgccatcgcc atcaacaagg
     1621 acgtcgacat cctcttcgtg atcgacaact ccgggtcgat gggtgaggag cagcagctgc
     1681 tctcggagaa cttcgcctcg ttcatcaacg tgctcgaggc caaggacgtc caggccaact
     1741 accgcatcgg catcaccacc acggacgccg gcaacccgcg ctgcccgacg gcgacctaca
     1801 agcccgaggg cggcaacctg gtgctctcga gctgcctcga ccgtattgag cagaacgagt
     1861 tcaagttcgg cgaggacgac ttctcggccg cctgcacgtc gaagtgcacc aagcgcgacg
     1921 ccgacctcac ggtccgcggc acgacgaccg cctacgacat gaacgaggtc ccgcgcaagt
     1981 ggatcgagcg catcgagacc gagtcgaaca tccagggcgt cgactcgaac gtcgaggcgt
     2041 tccagtgcta cggcccgcag ggcgtcgccg gctgcggctt cgagtcccac ctcgagagca
     2101 tgtacctcgc gctcgccaac gcgacgagct cggcctcgaa gaacaactac ggcttcctgc
     2161 gcgaggcggc gatcctctcg atcgtgttca tcacggacga ggcggactgc tcgtacaatc
     2221 cgctcacgaa ggagatcttc accaccaaca aggtgttctg gaagagcccg gatgacccgg
     2281 cgccgacctc ctcgctgtgc tggaaggccg gcgtcgagtg caccggtggc cccggcacct
     2341 actccgagtg ccactcccag aactacgacg tcacgggcgc ggtcaccacg gacccgacca
     2401 agatggcgct ccagccggtc tccaagtaca tcgacttcgt caagtcgatc gaggccgaga
     2461 agcagctgat cgacgagagc cagaacgtcc tggtctcgct gatcgccggc gtgcccaagg
     2521 gctacgagga tctccaggcc gagatcccgt acgaggactc cgccgacacc atgctgcagg
     2581 gcaacttcgg catcggcccg ggctgcgtgc tcgatgccgg caccgc
//
LOCUS       DASWNJ010000331         4105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_642186, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000331 DASWNJ010000000
VERSION     DASWNJ010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642186"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VGB85_05805"
     CDS             <1..259
                     /locus_tag="VGB85_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133570.1"
                     /translation="AWFQRKLQVRLMNAISRVTLAELRSVAGASGGVDLVRVRDDLGG
                     RIDAMLRARVEGGTRKLTLMLVAACIVLAIASTLAIQRIEW"
     gene            310..789
                     /locus_tag="VGB85_05810"
     CDS             310..789
                     /locus_tag="VGB85_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133571.1"
                     /translation="MAHPRARPSLLVLTLVLACGPDEKDEPEPEVMHCAELPEELGVL
                     RAIIDLDESAITIAIADETNLECSGPSTRWTGRLQLPPDIAPGAYALSESPPSLYVFD
                     YTIPSCMGSQSFGIDRYATGTVTISSVQAGCVAGEIEAVDEADDANTFKGAFAAEPR"
     gene            948..2462
                     /locus_tag="VGB85_05815"
     CDS             948..2462
                     /locus_tag="VGB85_05815"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0133572.1"
                     /translation="MDTEREAGQGESAAAHSAGNETVIDGQREALELAVHGAPLSAVL
                     DVIVRTVEAQSVNGVLGSILLLDAAAGRLHHGAAPSLPAAYNAAIDGIAIGPTIGSCG
                     TAAFTGRTIVVRDIATDPLWAAFKELALEHELRACWSTPILSSEDEVLGTFALYHRTP
                     TAPSERDREIVKLLGHTAGLVIERAQQAREREAAEAELRDTRERQIERIAAMFQHAPV
                     AIAVLRGPEHVFEVANPPYTDLVGGRALLGKTVREALPELAGLGFFELLDGVYRTGQP
                     HVGRSQPALIARSTGGPLEEAFVDFVYQPIPDADGVIDSIFVIAFEVTELARAKLEAE
                     AARAQAEASERNLRTFIDNLPELAWTATAEGHVDYYNRRYLEYTGLTHEEMQGWGWQQ
                     VQDPELLPAVVERWQRSLATGEPFEMEFTLRGHDGVPRWFLTRVAPARDNAGKVVRWF
                     GTNTNIQDMKAAQALSEAMVEQSRETQRMLLSMRADKERAERRVAELEAERAAR"
     gene            2483..3004
                     /locus_tag="VGB85_05820"
     CDS             2483..3004
                     /locus_tag="VGB85_05820"
                     /inference="COORDINATES: protein motif:HMM:NF018813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HEY0133573.1"
                     /translation="MALTATLYHLKLELSDVDRGVYTPLDLRLARLIAYCLAYTEGIA
                     FSKEGIASTDDPPIAVRDPTGQLLEWIDVGAPSAERLHRATRMARRVSLYTSAALAQL
                     QREAASRTIPRVEEIEVWRLEPAFLDAVDPRVDRNSKLELVRTDGLLYFTAGGEVIEG
                     TITRGSLVAGDPA"
     gene            3076..>4105
                     /locus_tag="VGB85_05825"
     CDS             3076..>4105
                     /locus_tag="VGB85_05825"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0133574.1"
                     /translation="MSKRAKKPRPERDQTDESLRTERRNSDKVLEQRRAAIEEAADET
                     LERARDQADAVLDTARDRADAELGLAQPGAQTPAAVVRERAREDEALQEERDAADRSV
                     EREREEHARLLAALLPLEREKTDRYLLTERARSDDAVEHRDDFLGMVSHDLRNLLSGI
                     VLNATLVLKRASGTDEGRRTAEGMQRIQRYVARMNRLIGDLIDVVSIDAGKLAVTLEP
                     HDASVLLGEAVDAFALVAAEKGIELSATAVAGPLPTRFDHDRMLQVLANLITNALKFT
                     AQGGQVVIEGARVGTDVRLSVSDTGTGIPAHMLEAVFDRFWQVGKNDQRGLGLGLYIS
                     RCIVEAHGG"
BASE COUNT          619 a         1429 c         1524 g          533 t
ORIGIN      
        1 cgcgtggttc cagcgcaagc tgcaggtgcg actgatgaac gcgatctcgc gggtgacgct
       61 cgccgagctt cgcagcgtcg cgggggccag cggcggcgtc gacctcgtcc gcgtgcgcga
      121 cgacctcggc gggcgcatcg acgcgatgct gcgcgcccgc gtcgagggcg gcacgcgcaa
      181 gctgacgctg atgctggtcg ccgcgtgcat cgtgctggcg atcgcgagca cgctggcgat
      241 ccagcggatc gagtggtgat cgggaccgac aggttgccat cacttcggga gtgcgtgatg
      301 atcgagacca tggcacaccc acgcgcccgc ccgtcgttgc tcgtcctgac gctcgtcctc
      361 gcctgcggcc ccgacgagaa ggatgagccg gagcccgagg tgatgcactg cgccgagctg
      421 ccggaggagc tcggggtcct gcgggcgatc atcgatctcg acgagtccgc gatcacgatc
      481 gcgatcgccg acgagacgaa cctcgagtgc agcggtccct cgacccggtg gaccggcagg
      541 ctgcaactgc caccggacat cgcgccgggc gcctacgcgc tgtccgagag ccccccgagc
      601 ctctacgtct tcgactatac gatccccagc tgtatgggca gccagagctt cggcatcgac
      661 cgttacgcga ctggcacagt gacgatctcg tcggtgcagg cgggctgcgt ggcgggcgag
      721 atcgaggccg tcgacgaggc cgatgacgcc aacaccttca agggcgcctt cgcggccgag
      781 ccccggtgat cccggcgcgg cgacgcgcga aaatgggcgc cgtcgccatc gtcccgcgcc
      841 agcggcgcag gcggcggtgc agggtctcgt gggtgacgtg gcgagcgtcg gccgtcgtgt
      901 ggcagcggtc atcggccggg gcgggggacc tgcggtaatg accggccgtg gacaccgaga
      961 gagaggcggg gcagggcgag tcggcggcgg cgcacagcgc cggcaacgag acggtgatcg
     1021 acggccagcg tgaggcgctc gagctcgcgg tccacggcgc gccgctgtcg gcggtgctcg
     1081 acgtgatcgt gcggacggtc gaggcgcagt cggtcaacgg cgtgctcggg tcgatcctgc
     1141 tgctcgacgc cgcggccggt cgcctgcacc acggcgccgc gccgagcctg ccggcggcct
     1201 acaacgcggc gatcgacggc atcgcgatcg gtccgacgat cgggtcgtgc ggcaccgcgg
     1261 cgttcaccgg gcggacgatc gtcgtccgcg acatcgccac tgacccgctg tgggcggcgt
     1321 tcaaggagct cgcgctcgag cacgagctgc gcgcgtgctg gtcgacgccg atcctgtcgt
     1381 cggaggatga ggtgctcggg accttcgcgc tgtaccaccg cacgccgacg gccccgagcg
     1441 agcgcgaccg cgagatcgtc aagttgctgg ggcacaccgc cggcctggtg atcgagcgcg
     1501 cgcagcaggc ccgcgagcgc gaggccgcgg aggccgagct gcgtgacacc cgcgagcggc
     1561 agatcgagcg catcgccgcg atgttccagc acgccccggt ggcgatcgca gtgctgcgcg
     1621 ggccggagca cgtgttcgag gtcgccaacc cgccctacac cgatctggtc ggcggccggg
     1681 cgctgctcgg caagaccgtg cgcgaggcgc tgccggaact cgcgggtctc ggcttcttcg
     1741 agctgctcga cggcgtgtac cgcacgggcc agccgcacgt cggccgctcg cagccggccc
     1801 tgatcgcgcg cagcaccggc ggcccgctcg aggaggcctt tgtcgacttc gtgtaccagc
     1861 cgatccccga cgccgacggg gtcatcgaca gcatcttcgt gatcgcgttc gaggtcaccg
     1921 agctggcgcg cgccaagctc gaggcggagg ccgcccgcgc gcaggccgag gcgagtgagc
     1981 gcaacctgcg gaccttcatc gacaacctgc cggagctggc ctggacggcg acggccgagg
     2041 gccacgtcga ctactacaat cgccgctacc tcgaatatac cggcctgacc cacgaggaga
     2101 tgcagggctg gggctggcag caggtgcagg accccgagct gctgccggcg gtggtcgagc
     2161 gctggcagcg gtcgctggcg accggggagc ccttcgagat ggagttcacg ctgcgcgggc
     2221 acgacggcgt gccgcggtgg ttcctgaccc gggtggcgcc cgcgcgcgac aacgccggca
     2281 aggtcgtgcg gtggttcggg accaacacca acattcagga catgaaggcc gcgcaggcgc
     2341 tgagcgaggc gatggtcgag cagagccgtg agacccagcg catgctgctg agcatgcgcg
     2401 ccgacaagga gcgggcggag cgtcgggtcg ccgagctcga ggcggagcgc gccgctcgct
     2461 gacgggttat cctcgcgcgc acatggccct gaccgcgacc ctctatcacc tgaagctcga
     2521 gctctcggac gtcgaccgcg gcgtctacac gccgctcgac ctccggctgg cgcggctgat
     2581 cgcctactgc ctcgcgtata ccgaggggat cgcgtttagc aaggagggca tcgcctcgac
     2641 cgacgacccg ccgatcgccg tgcgcgaccc gaccggccag ctgctcgagt ggatcgacgt
     2701 cggcgcgccc tcggcggagc ggctgcaccg ggccacgcgc atggcccggc gcgtgtcgct
     2761 gtacacctcg gccgcgctgg cccagctgca gcgcgaggcc gcgagccgga cgatcccgcg
     2821 ggtggaggag atcgaggtgt ggcgcctcga gccggccttc ctcgacgccg tcgacccgag
     2881 ggtcgaccgc aacagcaagc tcgagctggt gcgcaccgac ggcctgctgt acttcaccgc
     2941 cggcggcgag gtcatcgagg ggacgatcac ccgcggctcg ctcgtcgccg gcgacccggc
     3001 gtgagcgcgg gacgtcatcc ttcgcgcctg cgcagccgcg gagcaattgc cgggcaaacc
     3061 gggtaggatc ccgcgatgag caagcgggcc aagaagccgc gtcccgagcg cgaccagacg
     3121 gacgagagcc tgcggaccga gcggcgcaac agcgacaagg tgctcgagca gcggcgggcc
     3181 gcgatcgagg aggccgccga cgagacgctc gagcgcgcgc gagatcaggc cgacgccgtg
     3241 ctggacaccg cccgcgaccg cgccgacgcc gagctcggcc tggcccagcc cggcgcgcag
     3301 acccccgcgg cggtggtccg cgagcgcgcg cgcgaggacg aggcgttgca ggaggagcgc
     3361 gacgcggccg accgcagcgt cgagcgtgag cgcgaggagc acgcgcgtct gctcgccgcc
     3421 ctgttgccgc tcgagcgcga gaagacggac cgctatttgt tgacggagcg ggcgcgatcc
     3481 gacgacgcgg tcgagcaccg cgacgacttc ctcgggatgg tcagccacga cctgcgaaac
     3541 ctgctcagcg gcatcgtgtt gaacgcgacg ctggtgctca agcgggcctc ggggaccgac
     3601 gagggccggc ggacggcgga ggggatgcag cggatccagc gctacgtcgc gcgcatgaac
     3661 cggctgatcg gcgacctgat cgacgtcgtc agcatcgacg ccggcaagct cgcggtcacg
     3721 ctcgagccgc acgacgcgag cgtgctgctc ggcgaggccg tcgacgcgtt cgcgctcgtc
     3781 gccgccgaga aggggatcga gctgtcggcc acggccgtcg ccggcccgct gccgacccgc
     3841 ttcgaccacg accgaatgct tcaggtgctc gccaacctga tcaccaacgc gctcaagttc
     3901 acggcccagg gcggtcaggt cgtgatcgag ggggcgcgcg tcgggaccga cgtccgcctg
     3961 tcggtcagtg acaccggcac cggcatcccg gcgcacatgc tcgaggcggt cttcgaccgc
     4021 ttctggcagg tcggcaagaa cgaccagcgc gggctgggcc tcgggctcta catctcgcgc
     4081 tgcatcgtcg aggcgcacgg cggca
//
LOCUS       DASWNJ010000332         3332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_182848, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000332 DASWNJ010000000
VERSION     DASWNJ010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182848"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VGB85_05830"
     CDS             complement(<1..290)
                     /locus_tag="VGB85_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133575.1"
                     /translation="MASRLAASLLVWLALGCQAGAGERPAPASPAPPASAGEPAAAAE
                     PVPPSTVTAAVAPAPAAMPGPADAGDCPEKDDPAFGVLVAPNRPAVGQPMQIL"
     gene            630..1835
                     /locus_tag="VGB85_05835"
     CDS             630..1835
                     /locus_tag="VGB85_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase family protein"
                     /protein_id="HEY0133576.1"
                     /translation="MLVLIADKFPTQGREILARTGEVVYRPDLGEADLPAALAETHAD
                     ILVVRSTVVNAAAMHATPNLSLVIRAGAGVNTIDLKAASSRGIFVANCPGKNAIAVAE
                     LTMALLLALDRRIVDATLELRQGVWNKGKYGKARGIKGQTLGLVGFGIIAREVAVRAR
                     AFGMKLQTFEPYPLSPEVTEAFGVTQVASLEALLRGSDVVSVHVPYNKSTHHLIGAEQ
                     LGWMRPGSILLHTARGGVVDDTALAEAVRAGNLRAGLDVFEGEPAESKASYQGIAAAL
                     PDVYATPHIGASTEQAESAIAEEVLRIVHDYVSHGVVHHSVNLADRSARFSLVVRHLD
                     RVGVLAGVLSALREEQVNVQGMQNIIFAGGEAGCATITFEQEPSPALLQRLRDHEAIL
                     AVELRAARP"
     gene            1832..2218
                     /locus_tag="VGB85_05840"
     CDS             1832..2218
                     /locus_tag="VGB85_05840"
                     /inference="COORDINATES: protein motif:HMM:NF014016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HEY0133577.1"
                     /translation="MSDRFPDAIVLLAHGSPDPDWMTPVEATAALMRTQAPGTPVRTA
                     TLEHGRGLAEVAAELLAAGLARIAVIPLFLSPGGRHIKRDVPALVDSVQAQFPALSLR
                     LSPGAIGTDVPVLEALAQAALRRAGL"
     gene            complement(2247..2729)
                     /locus_tag="VGB85_05845"
     CDS             complement(2247..2729)
                     /locus_tag="VGB85_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY0133578.1"
                     /translation="MTTDNRQTPTVTDFMTSTVHTVSPDTEITEAVGTLISKGCSGAP
                     VVDAEGHVVGVLTHEGCMRALSGAAFNREPAGRVSDHMSREFVIAEPTSDLFHAVSVL
                     SNDGGRRVLIVADHKLQGIVTRTDALKALDKFRKEREQAGDGVNIETVAVGWSALTQQ
                     "
     gene            complement(2820..>3332)
                     /locus_tag="VGB85_05850"
     CDS             complement(2820..>3332)
                     /locus_tag="VGB85_05850"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HEY0133579.1"
                     /translation="LGHGHPDWEWPTTRDIIVHLTNFNEDYEPGEHHDIAGMVHAQGP
                     MLLSRKQVRFGFKKSDGHNVAVHELAHVLDLADGFADGTPSFWGSVPGWEALVQSRLK
                     KVRKGGREAPLRAYAGTNEAELFAVAVEAFFETPDKLRARDPALFTALTAVFNLDPAT
                     GALREPIDGA"
BASE COUNT          432 a         1188 c         1260 g          452 t
ORIGIN      
        1 aggatctgca tcggctgacc gaccgcgggc cggttcggcg cgacgagcac gccgaaggcg
       61 gggtcgtcct tctcggggca gtccccggcg tccgcgggcc ccggcatcgc cgcgggggcc
      121 ggcgcgaccg cggccgtgac ggtcgagggc gggacgggct ccgccgcggc cgcgggctcg
      181 ccagcgctgg caggcggggc cgggctcgcg ggcgcgggcc gctcgccggc acccgcctgg
      241 catccgagcg cgagccacac gagcagcgag gctgcgagcc gcgatgccat gcgagccggc
      301 ttgccaccgg cctggctccg cgcgagctgt cccggcgagg cggcgagccg ccatgccatg
      361 cgagccggcg gactgccgga gggcgaagcg ccggggcccc gcgccaccgc atccacgccg
      421 gcgtgcgagg tccggaccgg gccgcgtcgg ctccgtgcgc tggtcggctg cgtcaccggc
      481 acaggcatag cgggatctca ccggcgccgc cagatcggcg tacgcgaccg acgcccggct
      541 cccgggcgac gcgcggcccg ccgcccccga gcggcgccac gtgcccgggg cgcgcagcgg
      601 gcggcgcggc tgtgatatct ctggcgcgca tgctggtgct gatcgctgac aagttcccga
      661 cccagggtcg tgagatcctg gcccgcacgg gcgaggtcgt gtaccgcccc gacctcggtg
      721 aggccgacct gccggccgcg ctcgccgaga cccacgccga catcctggtg gtccggtcga
      781 ccgtcgtcaa cgccgcggcg atgcacgcga cgccgaacct ctcgcttgtg atccgcgccg
      841 gcgcgggtgt caacacgatc gacctcaagg cggccagctc gcgcggcatc ttcgtcgcca
      901 actgtcccgg caagaacgcg atcgcggtcg ccgagctgac gatggcgctg ctgctcgcgc
      961 tcgaccggcg gatcgtcgac gcgacgctcg agctgcgcca gggcgtgtgg aacaagggca
     1021 agtatggcaa ggcccgcggg atcaagggcc agacgctcgg cctggtcggc ttcgggatca
     1081 tcgcccgcga ggtcgccgtg cgcgcgcgcg cgttcggcat gaagctgcag accttcgagc
     1141 cctacccgct gtccccggag gtcacggagg cgttcggcgt cacgcaggtc gcctcgctcg
     1201 aggcgctgct gcgcggcagc gacgtcgtca gcgtgcacgt gccgtacaac aagtcgacgc
     1261 accacctgat cggcgccgag cagctcggct ggatgcgccc gggcagcatc ttgctgcaca
     1321 cggcgcgcgg cggcgtggtc gacgacaccg ccctcgccga ggcggtgcgc gcgggcaacc
     1381 tccgcgccgg cctcgatgtc ttcgagggcg agccggccga gagcaaggcg agctatcagg
     1441 gcatcgcggc cgcgctgccg gacgtctacg cgacgccgca catcggcgcg tcgaccgagc
     1501 aggccgagtc ggcgatcgcc gaggaggtcc tgcggatcgt gcacgactac gtcagccacg
     1561 gggtcgtgca ccacagcgtc aacctcgcgg atcgctcggc gcgctttagc ctggtcgtgc
     1621 gtcacctcga ccgcgtcggc gtgctcgctg gcgtgctgtc ggcgctgcgc gaggagcagg
     1681 tcaacgtgca gggcatgcag aacatcatct tcgccggcgg cgaggccggc tgcgcgacga
     1741 tcaccttcga gcaggagccg agccccgcgc tgctgcagcg cctgcgcgac cacgaggcga
     1801 tcctcgcggt cgagctgcgg gcggcccggc catgagcgac cgcttccccg acgcgatcgt
     1861 gctgctggcc cacggcagcc ccgaccccga ctggatgacg ccggtcgagg cgaccgcggc
     1921 gctgatgcgg acgcaggcgc cgggcacccc cgtgcgcacg gcgaccctcg agcacgggcg
     1981 cgggctcgcc gaggtcgcgg cggagctgct cgcggccggg ctggcgcgga tcgccgtgat
     2041 cccgctgttt ttgtcgccgg gtgggcgtca catcaagcgt gacgtgcccg cgctcgtcga
     2101 cagcgtgcag gcgcagttcc ctgccctctc gctgcggctc agcccgggcg cgatcgggac
     2161 cgacgtgccg gtgctcgagg cgctcgcgca ggcggccctg cggcgcgcgg ggctctgacc
     2221 cggggcccgc gcgcaggcgc cagcgctcac tgctgggtca gcgcggacca gccgacggcg
     2281 accgtctcga tgttgacgcc gtcgccggcc tgctcgcgct ccttgcggaa cttgtcgagg
     2341 gccttgaggg catcggtgcg ggtgacgatc ccctgcagct tgtggtcggc gacgatcagc
     2401 acgcggcggc cgccgtcgtt gctgagcacg gagacggcgt ggaacaggtc cgaggtcggc
     2461 tcggcgatca cgaactcgcg cgacatgtga tcggagacgc gaccggcggg ctcgcggttg
     2521 aaggcggcgc ccgacagcgc gcgcatgcag ccctcgtggg tcaggacgcc gacgacgtgg
     2581 ccctcggcgt cgacgacggg ggcgccggag cagcccttcg agatcagcgt gccgactgcc
     2641 tcggtgatct cggtgtccgg gctcaccgtg tgcacggtgc tggtcatgaa gtccgtgacg
     2701 gtgggcgtct gccggttgtc ggtggtcata gcggctcctc ctgcggccgc aagcgtaagc
     2761 caggcggccg gatgtgcaag cgcgtgcaag cgcgtgcgag cgagccggca ctgtcggcgt
     2821 cacgccccgt cgatcggctc gcgcagggcc ccggtcgccg gatcgaggtt gaacaccgca
     2881 gtcagcgcag tgaacagcgc cgggtcgcgg gcgcggagct tgtccggcgt ctcgaagaag
     2941 gcctcgaccg cgacggcgaa caattcggcc tcgttggtgc ccgcgtaggc gcgcagcggg
     3001 gcctcgcggc cgccctttcg cacctttttg agccgcgact ggacgagcgc ctcccagccc
     3061 ggcaccgagc cccaaaacga cggcgtgccg tcggcgaagc cgtcggcgag gtcgagcacg
     3121 tgcgcgagct cgtggacggc gacgttgtgg ccgtcggact tcttgaagcc gaagcggacc
     3181 tgcttgcgcg acagcaacat cgggccctgg gcgtggacca tgccggcgat gtcgtggtgc
     3241 tcgccgggct cgtagtcctc gttgaagttg gtcaggtgga cgatgatgtc gcgggtggtc
     3301 ggccactccc agtcggggtg gccgtgcccg ag
//
LOCUS       DASWNJ010000333         2756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_77250, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000333 DASWNJ010000000
VERSION     DASWNJ010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77250"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VGB85_05855"
     CDS             complement(<1..266)
                     /locus_tag="VGB85_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133580.1"
                     /translation="MRQQVFYSGVMSVVLTLLFGCDAPVEEVTARGESGGNGCSMWRC
                     GFNAAEVNGRSLQELHLGGLPNSDGVRIVGFVPPPLVLLGGYTLG"
     gene            complement(404..>2756)
                     /locus_tag="VGB85_05860"
     CDS             complement(404..>2756)
                     /locus_tag="VGB85_05860"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0133581.1"
                     /translation="RVPGPRWTEIVEAYVQAGRGLAAAHAAGLVHRDFKPSNAMIDAD
                     GRVRVLDFGLVRAQAGLDVTTVDASSSLHQMSLQLTHSGDVVGTPAYMSPEQIRGASI
                     GPASDQFSLCVALYEALYGHLPFAGDSLGSLFRSIQRHKLPEPPRGSRVPAWVRAALL
                     RGLHPVPEDRYASMDALLRALGASSTRRRRLGVGIGVGAAGVAALAGFLTARAQGPEL
                     CTGAATELAAVWNDAGRDGLGRALSAAGPAFDAELRPAVLAAIDEYAAAWTSSHRSAC
                     MARARGEQSDQMLDRRMACLAQRRTALGAALGVLTEGDAAVALEALRVVHDLPPVSRC
                     DEVVVLAAEVPPPVDPEVAVAVDEVRRALARAESLARAGKIPDALALADRGVEEALRS
                     EYRPLAAEALLVRGRLAINLALDRSDDHRLTRAMLTAIGSGMDEVAAEAAALAVFARS
                     RFTETAARALDDVPLAEELARRLAQPEAILGLLANNVGTVHLARGELAQARAAFARSL
                     ARREQALPADHPELANTLLNLALVEDDSAAREQHLRRALDIFASAFGPAHPQTMEVRI
                     MTARYLRDPNAAWALVLPGCEALGRFLPEDFGRRAFCLAELAHHEAEAGHVATAASHR
                     QQASALLAGRPAALPVGQTAIIEAEAARDRGEPDPGVAQLRAAIARYSHEAPSWWERR
                     DRAELLLVLGENLGPTAEAVATLTAAVADFEASKAQASDVYGEQCLARAHLRLAELLA
                     RGFGAKEAISRHLTAAETWYQAAGPAYAWRLQALADLRVNAHQ"
BASE COUNT          358 a          987 c         1085 g          326 t
ORIGIN      
        1 ccgagcgtgt agccgccgag cagcaccagc ggcggcggca cgaagccgac gatgcgcacg
       61 ccgtcgctgt tgggcaggcc gccgaggtgt agctcctgca gcgagcggcc gttgacctcg
      121 gcggcgttga acccgcagcg ccacatgctg cagccgttgc cgccggactc gccgcgggct
      181 gtgacctcct ccacaggagc gtcgcagccg aacaaaagtg tgaggaccac cgacatgaca
      241 cccgagtaga aaacttgctg acgcatgaca ggctccagga tcgggaatag cgggatctgc
      301 tcgcgccgac aatcctccct ggcgcggtac ccgacgccct ccggacctgg tgaacgttgt
      361 tcgggtaatt cgagcgcgcg aagccgcacg cggtgctacg ggcctactgg tgcgcgttca
      421 cgcgcagatc ggccagcgct tgcagacgcc aggcatatgc aggcccggcc gcctggtacc
      481 aggtctcggc ggccgtgaga tgtcgggaga tcgcttcctt ggcgccaaaa ccgcgcgcga
      541 gcagctcggc gagtcggagg tgcgcgcgcg cgaggcactg ctcgccgtag acgtcgctgg
      601 cctgcgcctt cgaggcctcg aagtcggcga ccgcggcggt cagcgtggcg acggcctcgg
      661 cggtcgggcc gaggttctcg ccgagcacga gcagcagctc ggcccggtcg cggcgctccc
      721 accagctcgg ggcctcgtgc gagtagcggg cgatcgcggc gcgcagctgg gcgacgccgg
      781 ggtccggctc gccgcggtcg cgggccgcct cggcctcgat gatcgcggtc tgtccgaccg
      841 gcagcgccgc ggggcggccg gccagcagcg cgctggcctg ctggcggtgc gacgccgcgg
      901 tcgcgacgtg gccggcctcg gcctcgtggt gggcgagctc ggcgaggcaa aacgcgcgcc
      961 gaccgaagtc ctccggcaag aaccgtccga gcgcctcgca gccgggcagc acgagcgccc
     1021 acgcggcgtt cgggtcacgc aggtaacgcg ccgtcatgat gcgcacctcc atcgtctgcg
     1081 ggtgggccgg tccgaacgcg ctggcgaaga tgtcgagcgc gcggcgcagg tgctgctcgc
     1141 gggcggcgct gtcgtcctcg acgagcgcga ggttgaggag cgtgttggcg agctcggggt
     1201 ggtcggccgg cagcgcctgc tcgcggcgag cgagcgagcg cgcaaaggcg gcccgcgcct
     1261 gcgccagctc gccgcgggcg aggtgcacgg tgccgacgtt gttggcgagc aggccgagga
     1321 tcgcctcggg ctgcgcgagg cgcctggcga gctcctcggc gagcggcacg tcgtcgaggg
     1381 cccgcgccgc ggtctcggtg aagcggctgc gcgcgaacac ggcgagcgcg gcggcctcgg
     1441 ccgcgacctc gtccatgccg ctgccgatcg ccgtcagcat ggcgcgcgtg agccggtggt
     1501 cgtcgctgcg gtcgagcgcg aggttgatcg ccaggcgccc gcggaccagc agggcctccg
     1561 cggcgagcgg gcgatactcg ctgcgcagcg cctcctcgac gccgcggtcg gcgagcgcga
     1621 gggcgtcggg gatcttgccg gcgcgcgcga ggctctcggc gcgcgcgagg gcccggcgca
     1681 cctcgtcgac cgcgaccgcg acctccgggt cgaccggcgg cgggacctcg gccgcgagca
     1741 cgacgacctc gtcgcagcgg ctgaccggcg ggaggtcatg gacgacgcgc agggcctcga
     1801 gcgcgaccgc ggcgtcgccc tcggtcagca cgccgagcgc ggccccgagc gcggtgcgcc
     1861 gctgggcgag gcaggccatg cgccggtcga gcatctgatc cgactgctcg ccgcgcgcgc
     1921 gggccatgca cgcgctgcgg tgggacgagg tccaggcggc ggcgtactcg tcgatcgcgg
     1981 cgagcaccgc gggccgcagc tcggcgtcga aggccggacc ggcggccgag agggcgcgcc
     2041 cgaggccgtc gcgcccggcg tcgttccaca ccgcggcgag ctcggtcgcg gcgccggtgc
     2101 acagctcggg gccctgggcg cgcgcggtca ggaagccggc gagggccgcg accccggccg
     2161 cgccgacgcc gatgccgacg ccgagccggc gccgccgcgt cgagctggcg ccgagcgccc
     2221 gcagcagcgc gtccatcgag gcgtacctgt cctcggggac aggatgcagc ccgcgcagca
     2281 gggcggcgcg cacccacgcc gggacccggg atccacgcgg cggctccggg agcttgtggc
     2341 gctggatcga ccggaacagc gagccgaggc tgtcgcccgc gaacggcagg tgcccgtaca
     2401 gcgcctcgta gagcgccacg cacaggctaa actggtcgct ggccgggccg atgctggcgc
     2461 cgcggatctg ctccggggac atgtacgcgg gcgtgccgac gacgtcgccc gagtgggtca
     2521 gctgcagcga catctgatgc aggctgctcg aagcgtcgac ggtggtgacg tcgagcccgg
     2581 cctgcgcgcg cacgagcccg aagtcgagca cgcgcacgcg gccgtcggcg tcgatcatcg
     2641 cgttgctggg cttgaagtcg cggtggacga ggccggcggc gtgggccgcc gcgagcccgc
     2701 gaccggcctg cacgtaggcc tcgacgatct cggtccagcg gggccccggc acgcgc
//
LOCUS       DASWNJ010000334         4686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_537055, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000334 DASWNJ010000000
VERSION     DASWNJ010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537055"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2546)
                     /locus_tag="VGB85_05865"
     CDS             complement(<1..2546)
                     /locus_tag="VGB85_05865"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0133582.1"
                     /translation="MAQGVGGGSDGLTLQLRQQLAAARAVAPDLEVQQLKASVRARLF
                     GIVDTPPMLGGYTIVRLIGRGGMGAVYEAHDERGEVVALKTLRGCEPAALVRFKHEFR
                     ALSDLVHPNLALLYSLAVTGDQAFLTMERIDGVDFLTYVGQAPAGELAPRLHGALAQL
                     IDGLGALHAAGKLHRDVKPSNVLVTREGRVVILDFGLVQELAGATIPGGEALAGTPAY
                     MAPELFLGAMPTTASDWYSVGVMLYEALAGTLPFTGTALAVLRDKCERDPPPPPDRGG
                     EGADTEALARLCMQLLERDPAARPDRKAILRALEAAAPGKGLAPGTGEPGRAARLRSP
                     AQAPLIGRQDELDVLRKAAAGVRSGEPAVVLVRGGSGLGKTTLVDSFLQELRGQAVIL
                     RGRCYEREQVPHNAFDGLVDALVGHLLTLDLTVQSELLTGDVEALVRLFPGLQRLPWP
                     ASDPRDAEDPAEVAATPDSRWRHRAYTAFKALLGAIAARTPVVLYIDDLHWADADSAT
                     LLAALFGAPAPSCLLLTSYRAGTSGPAPVVRGLLQQLRAGGVRVWELTLEPLTLDEAE
                     QLARALLGPGPAAASARMVARESAGIPLFVHELVHDVLGCISAGAGAFPARLEDLIGA
                     RVRQLPGPARDMLEMLAVAARPLARDLVIALSGQTATGRAALQTLGTAGLVRASRSER
                     GEQIEVCHDRTRETVLAGLDEARVRDCHRRLAATIEAHSLVPGETPIEPEILAHHLYA
                     AGDPAGARQHMITAASRAAKALAFARAAELSLAATRLCPPDDVAGRLALRIDAAQALM
                     HAGQLSDAAACFLEAAGEAPPVQALRLRQRAAEVLLNCGAPRRGMALLQEV"
     gene            2613..3230
                     /locus_tag="VGB85_05870"
     CDS             2613..3230
                     /locus_tag="VGB85_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0133583.1"
                     /translation="MTDFELLDAWRAGDREAGNTLFERYFDAVCRFFTNKVQHNIDDL
                     IQKTFLGCVEGRDRFQKQASFRTYLFAVAHHVLCAHFRERRRGPESTPIDFSDTSSDQ
                     LTPSPSSVLAIHQEQRLLLEALRRIPLDYQLVLELYYWEDMAAPELAQVLGLPEGTVR
                     SRLRRAKEALSAKMTALAAEPALLKSTTSSLEKWVCSLRAQLSGE"
     gene            3591..4157
                     /locus_tag="VGB85_05875"
     CDS             3591..4157
                     /locus_tag="VGB85_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY0133584.1"
                     /translation="MKISMLDTQMTTSMEARNSAGTWQKTAAADVEPATARPVPVVNL
                     LTELLQGYRRPAVVSTEQGTIVCMNAAAMRSIDRKPGDEDDGIPADPKEWPRRASIQA
                     EGQTFYLSVPNQALDTASGAPEPQLPPRLAKIARLVISGCTDKQIATRTGLSFSTVRT
                     YVRQIYRRIGVHSRVELVHASNTSLARD"
     gene            complement(4267..>4686)
                     /locus_tag="VGB85_05880"
     CDS             complement(4267..>4686)
                     /locus_tag="VGB85_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133585.1"
                     /translation="VGAAGERRHALLPVYQGWLDAQALRGGALVQIMVVEGNHDAGGA
                     GSSGSALFRVDPGLLRELAVRDDAELSADARATLDAGGPWSTYTLQRNGDAVARVIER
                     PLSPGGPTVVLVAAPPLAFEQAPALYARIVADAQRVP"
BASE COUNT          695 a         1866 c         1512 g          613 t
ORIGIN      
        1 acctcctgca gcagcgccat cccgcgtcgc ggcgccccgc agttgagcag cacctcggcc
       61 gcgcgctggc gcaggcgcag cgcctgcacc ggcggcgcct cgccggccgc ctcgaggaag
      121 cacgccgccg cgtccgagag ctgccccgcg tgcatcaacg cctgggccgc gtcgatgcgc
      181 agcgccagtc gcccggcgac gtcgtcgggc gggcacagcc tggtcgccgc gagcgacagc
      241 tccgccgcgc gcgcgaaggc cagcgccttg gcggcccggc tcgccgccgt gatcatgtgc
      301 tggcgcgcgc ccgccgggtc gcccgcggcg tacaagtgat gcgcgaggat ctccggctcg
      361 atcggcgtct cgcccggcac cagcgagtgc gcctcgatcg tcgcggcgag ccggcggtga
      421 cagtcgcgca cgcgcgcctc gtcgagcccc gcgagcaccg tctcgcgcgt gcgatcgtgg
      481 cacacctcga tctgctcgcc gcgctcgctg cgactggcac gcacgagccc cgccgtgccg
      541 agcgtctgca acgccgcgcg cccggtcgcc gtctgccccg acagcgcgat caccaggtca
      601 cgcgccagcg gccgggcggc gaccgccagc atctccagca tgtcgcgcgc cggccccggc
      661 aactgccgca cgcgcgcgcc gatcaggtcc tcgagccgcg ccgggaaggc ccccgcgccc
      721 gccgagatgc agccgagcac gtcgtgcacc agctcatgca cgaacagcgg gatcccggcc
      781 gactcgcgcg cgaccatcct cgcgctcgcg gccgccggcc ccggcccgag cagcgcccgc
      841 gccagctgct ccgcctcgtc gagcgtcagc ggctcgagcg tcagctccca cacccgcacc
      901 ccgccggcgc gcagctgctg caacagcccc cgcaccaccg gcgccggccc gctcgtgccg
      961 gcgcggtagc tcgtcaacag caggcacgac ggcgccgggg ccccgaacag cgcggccagc
     1021 agcgtcgcgc tgtcggcgtc ggcccagtgc aggtcgtcga tgtacagcac caccggcgtg
     1081 cgcgcggcga tcgccccgag cagcgccttg aaggccgtgt acgcgcggtg ccgccagcgc
     1141 gagtccggcg tcgccgcgac ctcggccggg tcctccgcgt cgcgtggatc actcgccggc
     1201 cacggtagcc gctgcagccc cgggaacagc cgcaccagcg cctcgacgtc gccggtgagc
     1261 agctccgact gcaccgtcag atcgagcgtc agcaggtgcc cgacgagcgc gtcgaccagc
     1321 ccatcgaacg cgttgtgcgg cacctgctcg cgctcgtagc agcggccgcg caggatcacc
     1381 gcctgcccgc gcagctcctg caagaagctg tcgaccagcg tcgtcttgcc gagccccgag
     1441 ccaccgcgca ccagcacgac cgccggctcg ccgcttcgca ccccggccgc cgccttgcgc
     1501 agcacgtcga gctcgtcctg ccggccgatc aacggcgcct gcgcagggct gcgcaggcgg
     1561 gccgcgcggc ccggctcacc tgtcccggga gccagcccct tgcccggcgc cgccgcctcg
     1621 agcgctcgca ggatcgcctt gcggtcgggc cgcgccgcgg ggtcgcgctc gagcagctgc
     1681 atgcacaacc gcgcgagcgc ctcggtgtcc gcgccctcgc ctccgcggtc cggcggcggc
     1741 ggcgggtcgc gctcgcactt gtcgcgcagc accgccagcg ccgtgccggt gaacggcagc
     1801 gtgccggcga gcgcctcgta gagcatcacg ccgacgctgt accagtcgct cgccgtcgtc
     1861 ggcatcgcgc cgaggaacag ctccggggcc atgtacgccg gcgtgccggc gagcgcctcg
     1921 ccgcccggga tcgtcgcgcc ggcgagctcc tgcaccagcc cgaagtcgag gatcaccacg
     1981 cgaccctcgc gggtgaccag cacgttcgac ggcttgacgt cgcggtgcag cttgcccgcc
     2041 gcgtgcaacg ccccgagccc gtcgatcagc tgggccagcg caccatgtaa gcgcggcgcc
     2101 agctcgccgg cgggcgcctg tccgacatat gtcaggaagt cgaccccgtc gatccgctcc
     2161 atcgtcaaaa acgcctggtc gccggtgacc gccaggctgt agagcagcgc caggttcggg
     2221 tgcacgagat cgctgagggc gcgaaactcg tgcttgaagc ggaccagcgc cgcgggttcg
     2281 cagccccgca gggtcttgag ggcgacgacc tcgccgcgct cgtcgtgggc ctcgtacacc
     2341 gcccccatcc cgccgcgacc gatcaatcgc acgatcgtgt atccaccgag catcggcggc
     2401 gtatcgacga tgccgaacag ccgcgcgcgc accgacgcct tgagctgctg gacctccagg
     2461 tccggcgcga ccgcccgcgc ggccgcaagc tgctggcgga gctgaagtgt caggccatcc
     2521 gacccgccgc caaccccttg cgccatggcc catcgtacca tgacggcaac acagccggcc
     2581 cggacgatga tactatcgcc gcgacagcct tggtgacgga cttcgaactc ctggatgcat
     2641 ggcgggccgg cgaccgtgaa gccggcaata cgctgttcga gcgctatttt gacgcggtgt
     2701 gccgcttttt caccaacaag gtgcagcaca acatcgacga cctgatccaa aagaccttcc
     2761 ttggatgcgt cgagggtcgt gaccgcttcc aaaagcaagc tagcttccga acctacctgt
     2821 ttgcagtcgc acatcatgtc ctgtgcgccc acttccgcga gcgccggcgc ggccccgaga
     2881 gcacgccgat cgatttttcg gacacctcga gcgaccagct gaccccctcg ccgagcagcg
     2941 tcctggcgat ccatcaagag cagcgcctgc tgctcgaggc cctgcgccgg atccccctcg
     3001 attatcagct cgtgctcgag ctctattact gggaagacat ggccgcgccc gagctcgccc
     3061 aggtcctcgg cctgcccgaa ggcaccgtcc gctcccgcct gcgccgcgcc aaagaggcgc
     3121 tgtcggcgaa gatgaccgcc ctcgccgccg agcccgccct gctcaagtcg accaccagct
     3181 cgctcgagaa atgggtctgc tcgctgcgcg cccagctcag cggcgagtga ccatataacg
     3241 cgtttcagtc cggtccgacc gcgaggtacc acgatggtcc acgcgtacgg gcgagtcgta
     3301 catcgcgacc actcgccgac cgacgcccga cctcgtcgcc cgcgccgcca agggagcccc
     3361 tggacgggct cgtcgggcgc caattcctgc gtatacgtca tcagttcagg aagttgggat
     3421 cggtgacgtc ggcgcggaca ctacccaccc cggctgttgc tcggcggcat gagctctgtt
     3481 catgcgacgc ggcgttcccg gcgccttcac cggacacatt ccacggtcct cccggaccgc
     3541 cactcccacg gtcctccgac cgccactctc cacggtcctc cggacctgaa atgaagatct
     3601 cgatgctcga cacccagatg accacctcca tggaagctcg taactctgca ggaacctggc
     3661 aaaagaccgc cgccgcggac gtcgaaccgg cgacggcacg acctgtcccg gtcgtcaacc
     3721 tcctgaccga gctgctgcag ggctatcgcc gccccgccgt cgtcagcacc gagcagggga
     3781 ccatcgtatg tatgaacgcc gcggcgatgc gcagcatcga ccgcaagccc ggcgacgagg
     3841 atgacggcat cccggccgac cccaaggagt ggccgcgccg cgcctcgatc caggccgagg
     3901 gtcagacctt ctacctctcc gtgcccaacc aggccctcga caccgccagc ggcgcccccg
     3961 agccgcagct gccgccgcgc ctggcgaaga tcgctcgcct cgtgatctct ggctgcaccg
     4021 acaagcagat cgcgacccgc accggcctct cgtttagcac cgtgcgcacc tacgtgcgtc
     4081 agatctaccg ccggatcggc gtgcacagcc gcgtcgagct cgtccacgcc agcaacacca
     4141 gcctcgcccg cgactaggcc acgccctcgc gtccttcggc cacgagcccg cgtcctcgag
     4201 agcgcggcgc tcgtggctgt tttcatgggg cctgtcccat gggacagctt acgacagcgg
     4261 acaggctcac ggcacgcgct gcgcgtcggc gacgatccgg gcatacagcg ccggcgcctg
     4321 ctcgaaggcc agcggcggtg cggcgacgag gaccaccgtc gggccgccgg gtgagagcgg
     4381 ccgctcgatg acccgcgcga ccgcatcacc gttgcgctgc agggtgtagg tcgaccacgg
     4441 accgcccgcg tcgagcgtcg cgcgggcgtc cgccgacagc tccgcgtcgt cacgcaccgc
     4501 gagctcgcgc agcaggcccg ggtcgacgcg gaacagcgcc gaccccgagc tgcccgcgcc
     4561 gcccgcgtcg tgattaccct cgacgaccat gatctgcacg agcgccccgc cgcgcagcgc
     4621 ctgcgcgtcg agccagccct gatacaccgg cagcagcgcg tggcggcgct caccggccgc
     4681 gccgac
//
LOCUS       DASWNJ010000335         1595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_571571, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000335 DASWNJ010000000
VERSION     DASWNJ010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571571"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..386
                     /locus_tag="VGB85_05885"
     CDS             114..386
                     /locus_tag="VGB85_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY0133586.1"
                     /translation="MATIRIPTPLRKYTQGQEEVQVAGGTVHAVIEALETSYPGIRER
                     ICDDSGKVRRFVNVFVGDEDIRFLENLNTPVAERDEISIIPAIAGG"
     gene            388..1377
                     /locus_tag="VGB85_05890"
     CDS             388..1377
                     /locus_tag="VGB85_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HEY0133587.1"
                     /translation="MRSVSPTARAQLQEQLRPARAIRVDNLTDLVGNTPLVRLHHIEA
                     ACPGVELWAKCEFTNPAGSVKDRAALRIVRQALTEGKLRPGMRLLDSTSGNTGIAYAM
                     VGAALGVPITLVMPENVSVPRKQVATAYGVEIVYSSPMEGSDGAIVLAKQLAREQADR
                     YWYADQYSNPNNPLAHYHSTAPEIWAQIGGRVTHFVAGIGTSGTVIGTSRRLHELGPQ
                     VRCLGLQPDDAMHGLEGMKHLPSSIVPKIYDEAALDGMIWMPTEEGWDMAERLSHEEG
                     LLVGHSSGGNVAGALRVAKALSTEGQGGVVVTILCDRGDRYFLPLKWERQYLW"
     gene            1383..>1595
                     /locus_tag="VGB85_05895"
     CDS             1383..>1595
                     /locus_tag="VGB85_05895"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HEY0133588.1"
                     /translation="MSEADRIPPALLDAIYAQARAEFPNECCGYIRGRGADMKLVQCK
                     NWQDRLHAVDPEANPRTAANGYNFGGR"
BASE COUNT          260 a          561 c          546 g          228 t
ORIGIN      
        1 atccccgccg gtccggtcgc gcaggccggt cgcccggtcg acactggcgc ctgagacccg
       61 cgcggcgctt gcgattgtgc cggaccttgt tcactattct gaacggaatt tccatggcta
      121 ccattcgcat ccccactccc cttcgcaagt acacccaggg tcaggaagag gtgcaggtcg
      181 ccggcggcac ggtgcacgcc gtgatcgagg cgctcgagac cagctacccc ggcatccgcg
      241 agcgcatctg cgacgacagc ggcaaggtgc gccgcttcgt caacgtgttc gtcggcgacg
      301 aggacatccg tttcctcgag aacctcaaca cgccggtggc cgagcgcgac gagatcagca
      361 tcatccccgc gatcgccggc ggctgacatg cgctccgtgt cgccgaccgc ccgggcccag
      421 ctgcaggagc agctgcgccc ggcgcgtgcc attcgcgtcg acaacctgac cgacctggtc
      481 ggcaacacgc cgctggtccg gctgcatcac atcgaggccg cctgccccgg ggtcgagctg
      541 tgggccaagt gcgagttcac caacccggcc ggctcggtca aggatcgcgc ggcgctgagg
      601 atcgttcgtc aggcgctcac cgagggcaag ctgcgtcccg gcatgcgcct gctcgacagc
      661 accagcggca acaccggcat cgcctacgcg atggtcggcg cggcgctcgg cgtgccgatc
      721 acgctggtga tgcccgagaa cgtcagcgtg ccgcgcaagc aggtcgcgac cgcgtacggg
      781 gtcgagatcg tgtactcgag cccgatggag ggcagcgacg gggcgatcgt gctggccaag
      841 cagctcgctc gcgagcaggc ggatcgctac tggtacgccg accagtactc gaaccccaac
      901 aacccgctcg cgcactacca ctcgaccgcg cccgagatct gggcgcagat cgggggccgc
      961 gtgacgcact tcgtcgccgg catcggcacc agcggcacgg tgatcggcac ctcgcggcgc
     1021 ctgcacgagc tcggcccgca ggtgcgctgc ctcgggctgc agccggacga cgcgatgcac
     1081 ggcctcgagg gcatgaagca cctgccctcg agcatcgtgc cgaagatcta cgatgaggcc
     1141 gcgctcgacg gcatgatctg gatgccgacc gaggagggct gggacatggc cgagcgcctc
     1201 tcgcatgagg aggggctgct ggtgggccac tcgtccggcg ggaatgtggc cggggccctg
     1261 cgcgtcgcca aggcgctttc gaccgagggt cagggcggcg tggtcgtgac gatcctgtgc
     1321 gaccgcggcg accgctattt cctgccgctc aagtgggagc gacaatacct gtggtgagca
     1381 gcatgtccga agccgatcgc atcccccctg ccctgctcga cgcgatctac gcccaggcgc
     1441 gcgccgagtt cccgaacgag tgctgcggct acatccgcgg gcgcggcgcg gacatgaagc
     1501 tcgtgcagtg caagaactgg caggaccggc tgcacgcggt cgacccggag gccaacccgc
     1561 gcaccgcggc gaacgggtac aacttcggcg ggcgc
//
LOCUS       DASWNJ010000336         1744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_676522, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000336 DASWNJ010000000
VERSION     DASWNJ010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676522"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /gene="ilvB"
                     /locus_tag="VGB85_05900"
     CDS             <1..1365
                     /gene="ilvB"
                     /locus_tag="VGB85_05900"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEY0133589.1"
                     /translation="KHNFLVTDPAEIPRTVAEAFHIASTGRPGPVLVDVAKSALQAMT
                     NHDWPTELSLPGYRPTTRPHTKQIREAARLILESRRPVLYVGGGVIRAAASRELKVLA
                     ELTGIPVVTTLMARGAFPDSHPQHLGMPGMHGTVAAVAGLQRSDLIISLGARFDDRVT
                     GNLDSFAPHAKVIHADIDPAEIGKNRYTDVPIVGDCREVISELVVALTADADSGNTGD
                     YEGWVAFLADVKRRYPLGYDAPVDGSLAPQLVMERLGAIAGPDAVYCSGVGQHQMWAA
                     HYVGYEKPNTWINSGGLGTMGFAVPAAMGAKVGRPDATVWAIDGDGCFQMTNQELATC
                     AIEGIPIKVAIINNESLGMVRQWQTLFYNERYSNTDLHSRRIPDFVKLAEAYGCVGLS
                     CDSPADVDATIAKAMAVDDVPVVVDFRVHRDAMVWPMVAAGTSNDDIKYARDLAPEFD
                     QDDL"
     gene            1436..>1744
                     /gene="ilvN"
                     /locus_tag="VGB85_05905"
     CDS             1436..>1744
                     /gene="ilvN"
                     /locus_tag="VGB85_05905"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEY0133590.1"
                     /translation="MSKHTLSVLVENKPGVLARIAGLFSRRGFNIDSLAVGPTEHPEV
                     SRMTIVVNVEESPLEQVTKQLNKLVEVIKIVELDPAASVQRELVLVKVGATPETRSQV
                     L"
BASE COUNT          279 a          623 c          592 g          250 t
ORIGIN      
        1 aagcacaact tcctggtcac cgatcccgcc gagatcccgc gcacggtggc cgaggccttc
       61 cacatcgcct cgacggggcg cccggggccg gtgctggtcg acgtggcgaa gtcagcgctc
      121 caggcgatga ccaaccacga ctggccgacc gagctgtcgc tgccgggcta ccgcccgacc
      181 acccgcccgc acaccaagca gatccgcgag gcggcccgac tgatcctgga gtcccgacgc
      241 cccgtcctgt acgtcggcgg tggcgtgatc cgagcggccg cgtcccgcga gctcaaggtg
      301 ctcgccgagc tgaccggcat cccggtcgtg accacgctga tggcacgcgg ggcgttcccg
      361 gacagccacc cgcagcacct cggcatgccg ggcatgcacg gcaccgtcgc ggccgtcgcg
      421 gggctgcagc gctccgacct gatcatctcg ctgggtgcgc ggttcgacga ccgcgtcacc
      481 ggcaacctcg actcgttcgc gccgcacgcg aaggtgatcc acgccgacat cgaccccgcg
      541 gagatcggca agaaccgcta caccgacgtg ccgatcgtgg gtgactgccg ggaggtgatc
      601 agcgagctcg tcgtcgccct caccgccgac gccgactccg gcaacaccgg tgactacgag
      661 ggctgggtcg ctttcctcgc cgacgtgaag cggcggtacc cgctcgggta cgacgcgccg
      721 gtcgatggct cgctggcacc gcagctggtg atggagcggc tcggcgcgat cgccggcccc
      781 gacgccgtct actgctcggg ggtgggccag caccagatgt gggccgcgca ctacgtcggc
      841 tacgagaagc cgaacacgtg gatcaactcc ggcggcctgg gcaccatggg cttcgccgtg
      901 ccggcggcga tgggcgccaa ggtggggcgc cccgacgcga ccgtctgggc gatcgacggc
      961 gacggctgct tccagatgac caaccaggag ctcgccacgt gtgcgatcga gggcatcccc
     1021 atcaaggtgg cgatcatcaa caacgagtcg ctggggatgg tccggcagtg gcagacgctc
     1081 ttctacaacg agcggtactc caacaccgat ctgcactcgc gccggatccc ggacttcgtg
     1141 aagctggccg aggcctacgg ctgcgtcggc ctctcctgcg actcgccggc cgacgtcgat
     1201 gcgaccatcg ccaaggcgat ggcggtcgac gacgtcccgg tcgtcgtcga cttccgggtg
     1261 caccgggatg cgatggtgtg gccgatggtc gcggcgggca cgtcgaacga cgacatcaag
     1321 tacgcccggg acctcgcgcc cgagttcgac caggacgacc tgtgatgacc ccggcgagcc
     1381 gctcggtgct ccgctcgcgt cgcgggcgag tccggatgac cacgcgaggc ccacgatgag
     1441 caagcacacc ctctcagtgc tggtcgagaa caagcccggc gtgctggcgc gcatcgcggg
     1501 cctcttctcg cgccgcggtt tcaacatcga ctcgctcgcg gtcggtccca ccgagcaccc
     1561 cgaggtctcc cggatgacga tcgtcgtcaa cgtcgaggag tccccgctcg aacaggtcac
     1621 caagcagctc aacaagctgg tcgaggtgat caagatcgtc gagctcgacc cggcggcgtc
     1681 ggtccagcgg gagctggtgc tggtcaaggt cggcgcgacg cccgagacgc ggtcgcaggt
     1741 cctg
//
LOCUS       DASWNJ010000337         1822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_500309, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000337 DASWNJ010000000
VERSION     DASWNJ010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500309"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(412..>1822)
                     /locus_tag="VGB85_05910"
     CDS             complement(412..>1822)
                     /locus_tag="VGB85_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133591.1"
                     /translation="YQSLLAPRRKELHCKVGSAMEALFADRLAELYPIIGRHALLGEE
                     WARAADYLGRSGAAAARLHAHVEARIHYGDALRALDNLPADDTTDRALIDLTVALSKV
                     SLVADEPTVNLARLARAETRARVLLARGGSPEDRVRLARIELQQGNSLALRGDSYAAA
                     MACFERVLPVAQELQDEELLVAPSTMVGRAMVTRGLFNAAIPLFRRVLRPLERGGDVT
                     EYILTALFLALAETAVGRWQEGAQIAGQALARAVETNNATARTYGHMFLAVMAMHTGD
                     MPRVLMESRQALLLADSSGNRMYCYFSHGYLALAHSRLGEHAAAAEWLAQQHISAASL
                     GGTLVTGDWFAAIEAEIALDAGDVVGALRLARAAIDLAGSIGGIYGGALARRVCGAAL
                     FAAGEAPRASIDEQFSASIELLEQGGATLELARTHARWGHADPDHAHERWSRALALYE
                     AAQLEAEAAPLRAAIAGVA"
BASE COUNT          259 a          688 c          635 g          240 t
ORIGIN      
        1 cggtcgagca tgcgggtgat cgcgccggtc gtcagacccg tgctcgccgc cagctcgccc
       61 gccgtcaccg ccccggtctc cgccaggcgc tgcagacagc gcaggtcggt cacgtgaatc
      121 ccgagccggt cggcgacggt ctggtgcagg aacaccgcgt ccgtcgccat gcggggcccc
      181 gcccgctgga gccccgccag cagctccgcg cgcctcgccg acgccgccct ggccccgcgc
      241 gcccgcggtg gaccgcccgt caccacgaaa ccaccgggcc gcggattttg agtgagccag
      301 gaaacctctg caccgtgcag ataatgtacg ggacaaatac atgcacgtca aggcggcagc
      361 gcgaccagtc catgcgatca tgctcgagcg ctcctcgaaa gctatgacgg ttcaggccac
      421 gccggcgatc gcagcgcgca gcggcgccgc ctcggcctcg agctgcgccg cctcgtacag
      481 cgccagcgcc cgcgaccagc gctcgtgggc gtggtcggga tcggcgtggc cccatcgcgc
      541 gtgggtccgg gccagctcga gcgtcgcgcc gccctgctcg agcagctcga tgctcgccga
      601 gaattgctcg tcgatgctcg cgcggggcgc ctccccggcg gcgaacagcg ccgcgccgca
      661 gacccttcgg gccagcgcgc cgccgtagat gccgccgatc gacccggcca gatcgatcgc
      721 cgcccgggcc agacgcagcg caccgacgac gtcgccggcg tcgagcgcga tctcggcctc
      781 gatcgcggcg aaccagtcgc cggtcaccag cgtaccgccg aggctggcgg cgctgatgtg
      841 ctgctgcgcc agccactccg ccgccgccgc gtgctcgccg aggcggctgt gcgcgagcgc
      901 cagatatccg tggctgaaat agcagtacat gcggttgccc gacgagtcgg ccaacagcag
      961 cgcttgccgg ctctccatga ggacccgcgg catatcgccg gtgtgcatcg ccatcaccgc
     1021 gaggaacatg tggccgtagg ttcgggcggt cgcgttgttg gtctcgaccg cgcgcgccag
     1081 cgcctggccg gcgatctgcg cgccctcctg ccagcggccg accgccgtct cggccagcgc
     1141 gaggaacagc gcggtcaaga tgtactccgt gacgtcaccg ccgcgctcga gcggccgcag
     1201 cacccggcga aacagcggga tcgcggcgtt gaacagcccg cgggtgacca tcgcccggcc
     1261 gaccatcgtc gacggggcga ccagcaattc ctcgtcctgg agctcctgcg cgaccgggag
     1321 cacccgctcg aagcaggcca tggcggccgc ataggagtcg ccgcgcaggg ccagcgagtt
     1381 gccctgctgc agctcgatgc gagccaggcg cacgcggtcc tccggcgatc cgccgcgtgc
     1441 cagcagcacc cgggcccgcg tctcggcgcg cgcgaggcgg gccaggttga ccgtcggctc
     1501 gtccgcgacg agcgagacct tgctcagcgc gaccgtcagg tcgatcagcg cgcggtcggt
     1561 cgtgtcgtcg gcgggcaggt tgtcgagcgc ccggagggcg tcgccgtagt gaatgcgggc
     1621 ctcgacgtgc gcgtgcaggc gggccgccgc cgcgcccgac ctcccgagat agtcggcggc
     1681 cctcgcccac tcctccccga gcagcgcgtg tcgtccgatg atcggataga gctcggcgag
     1741 gcgatcggcg aacagcgcct ccatggcgga cccgaccttg caatgcagct ctttacgccg
     1801 gggcgccagc aggctctgat ag
//
LOCUS       DASWNJ010000338         5519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_217988, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000338 DASWNJ010000000
VERSION     DASWNJ010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217988"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VGB85_05915"
     CDS             <1..508
                     /locus_tag="VGB85_05915"
                     /inference="COORDINATES: protein motif:HMM:NF018525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0133592.1"
                     /translation="WLGRALAAEPGRPYDPVVALDLPEPALPRLDTWPPAISAALGDN
                     FHDLAATVVLAHSVGCQATLHALAALPRGARVSAMVAVAGWWEVDEPWPAIVPWQTSL
                     PDLERVRGAVGKLVVLLSDDDPFTRNHIANAALWRNRLDAEVVLIPGAKHFNTDAAPE
                     VLETMRTL"
     gene            662..4012
                     /locus_tag="VGB85_05920"
     CDS             662..4012
                     /locus_tag="VGB85_05920"
                     /inference="COORDINATES: protein motif:HMM:NF020076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEY0133593.1"
                     /translation="MPRILSVVALSLVSIASLSCTAKPSRGDIASEPTGECTLEWREP
                     EAQGDVVAPFSLTSSEGIGLELLSLDARAVVDDPLAFTELTMVFRNPEARVIEGRFEI
                     TLPPGAAISRFAMKINNDWMEAEVVELQAARRAYEDALHRRQDPALLEKNAGNRFSAR
                     VFPIPANGVKEIIVSYSEALASNSEPYRLHLRGLPKLKDLGVDVVVPRASGPEHLGIR
                     EKDFMPQKDLELVTARRSAEVGLRYERLAVARVAPDIKLPQEPISGVTLLFDTSASRA
                     VDFKGQVARLGKIIEELRAATGNDFDLRVLCFDQTVEPVFAGKASSFSADAQQAILKR
                     NALGASDLAGALKYVADNPTRHERLIVVSDGIATAGDTERDKLRAAAGALGKAGVRRI
                     DAVVDGGVQDEISLKQITGGNLERDGIVVNARLPAALLASRLVRATRSDLRVRVPGAT
                     WVWPETLDAVQPGDQVLIFADLPAGAPMHVELGDTGEGARDVPLKNAERPLLERAWVK
                     ANIDRLSAALDTSDAGKAETRKQILDLSIKYRVLSDYTALLVLETEYDYQRFGIDRSA
                     LAEILTVGPSGVTTIDRSRLPNKGPGFAGEERSEEERSVLDNLFGDDKARVATRSKGA
                     GAEPPGAPTVEGATGRPAPTDPALDGLPAPAAAMAPGGGGDTGIGGMPERDESIGGAP
                     PRLAAQGAPPPRTPEPMAEAAKDEAEESDDRPSMDAKRKSARPTGARPQQDPFDVPTP
                     RVRPRPIAPRPLQPTPPPPPRPIERPRKTDAYDGRYAIVMSQVDQHNPGGALREAWLW
                     REQSPGDELALLALGEAAEASGDAPLAARAYGSLIDLFPGRADIRRMAGERLEALGPV
                     GLGLAVDTYAQAVQQRPDHPSSHRLYAFALLKAGRHAEAFAAALKGAQQAYPWGRFAG
                     VERILADDLRLIAAAWLAERPMDEKTIRAELGRANVTPDTAPSLRFVLNWETDANDVD
                     FHIYDGQGGHAYYSNRSLPSGGELYADVTTGYGPECFTVPAQASAFPYVLQGHYYSRG
                     PMGYGMGKLEVIEHDGLGHLRFKEHPFVIMKDGSFVDLGTLDGPLAKAGPGARLEPGD
                     HGAAGKPGPAGSYTPPPPVGQPLPPTRF"
     gene            4118..>5519
                     /locus_tag="VGB85_05925"
     CDS             4118..>5519
                     /locus_tag="VGB85_05925"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0133594.1"
                     /translation="MKTLTLINLASVAALAAFLVGCGDNGGTSVSASGSASQSSTDPG
                     SSGPETPTTGGSGSLSATESEAGSISESNSMGDTVTVGGVSEGVSMSSDPGTTTGIGG
                     GGCGDNILDPGEQCDNGPDNGPGQLCKLDCTDNVCGDNDQGPTEACDNGVDNGDNASC
                     KLDCQPAVCGDGLVGPGEGCDDGNQDDADECTNTCKAKDCGDGVVAATEQCDDANDID
                     DDACTNNCTAAICGDGFKQGGIGEECDDGNNNSNTGQCTLGCLLAQCGDAFVFTDGGN
                     EQCDLGANNGPGKPCNAQCKINVCGDGDQKPDEACDDGNLQGGDGCSQFCVLEECGNN
                     IVDPGEQCDDGKNGNNADSCTDFCLAPACGDGFVQVNKGEDCDNGADNDNNGACTLQC
                     KQADCGDGFVYIGVEQCDDGPNNSNTGNCKENCSKQICGDGFKGPGEACDDGNLSNFD
                     SCTNVCKQAGCGDGFKQ"
BASE COUNT          890 a         2193 c         1763 g          673 t
ORIGIN      
        1 gtggctcggg cgcgcgctcg cggccgagcc cgggcggcct tacgacccgg tcgtcgcgct
       61 cgacctcccg gagcccgcgc tcccgcgcct cgacacctgg ccgcctgcga tctcggccgc
      121 gctgggcgac aattttcatg acctcgccgc gaccgtcgtg ctcgcacaca gcgtcggctg
      181 ccaggcgacc ctgcacgccc tcgctgcatt gccgcgaggc gcgagggtct cggcgatggt
      241 cgcggtggca gggtggtggg aggtcgacga gccctggccg gcgatcgtgc cctggcagac
      301 cagcctcccg gatctcgagc gtgtgcgcgg tgctgtcggc aagctcgtcg tcctgctctc
      361 cgacgacgac cccttcaccc gcaaccatat cgccaacgct gccctgtggc gcaaccgcct
      421 cgacgccgag gtcgtgctga tcccgggcgc gaagcacttc aacaccgacg cggcgccgga
      481 ggtgctcgaa accatgcgaa cgctgtgaac caccgcgggc ttacagcccc tctgcacttt
      541 ctcatcaccc tcttcgcggg cgacgacgcg cgtcgatcca ctaaacccat cgctggatct
      601 ccgcgttgtc ggggccccgc gcgagcctcg atcccgctac gctcgcgagt ctggagtctg
      661 tatgccccgc atcctgtccg tcgtcgccct gagcctggtc tcgatcgcgt ccctgtcgtg
      721 caccgcgaag ccctcccgcg gagacatcgc cagtgaacca accggcgagt gcacgctcga
      781 gtggcgcgag cccgaggccc agggtgacgt cgtcgccccc ttctcgctca cctccagcga
      841 gggcatcggc ctcgagctgc tctcgctcga cgcccgcgcc gtcgtcgacg acccgctcgc
      901 cttcaccgag ctgacgatgg tgttccgcaa ccccgaggcg cgcgtgatcg agggccgctt
      961 cgagatcacg ctcccgcccg gcgcggccat ctcgcgcttc gccatgaaga tcaacaacga
     1021 ctggatggag gccgaggtcg tcgagctgca ggcggcccgc cgcgcctacg aggacgccct
     1081 gcaccgccgc caggaccccg ccctgctcga gaagaacgcc ggcaaccgct tcagcgcccg
     1141 cgtgttcccg atccccgcca acggcgtcaa ggagatcatc gtctcgtact cggaggccct
     1201 cgccagcaac agcgagccct accgcctgca cctgcgcggc ctgccgaagc tcaaggacct
     1261 cggcgtcgac gtcgtcgtcc cgcgcgcctc cggccccgag cacctcggca tccgcgagaa
     1321 ggacttcatg ccgcaaaagg acctcgagct ggtgaccgcg cggcgcagcg ccgaggtcgg
     1381 cctgcgctac gaacgactcg cggtcgcccg cgtcgccccc gacatcaagc tcccgcagga
     1441 gccgatctcc ggcgtcaccc tgctattcga caccagcgcc tcgcgggccg tcgacttcaa
     1501 gggacaggtc gctcgcctcg gcaagatcat cgaggagctg cgcgccgcga ccggcaacga
     1561 cttcgacctg cgcgtgctct gcttcgatca aaccgtcgag cccgtgttcg ccggcaaggc
     1621 cagcagcttc agcgccgacg cccagcaggc gatcctcaag cgcaacgccc tcggcgcctc
     1681 cgacctcgcc ggcgcgctca agtacgtcgc cgacaacccg accaggcacg agcgcctgat
     1741 cgtcgtcagc gacggcatcg ccaccgccgg cgacaccgag cgcgacaagc tccgcgccgc
     1801 cgccggtgcg ctcggcaagg ccggcgtgcg ccgcatcgac gccgtcgtcg acggcggcgt
     1861 gcaggacgag atctcactca agcagatcac cggcggcaac ctcgagcgcg acggcatcgt
     1921 cgtcaacgcc cgcctgcccg ccgcgctgct cgcctcgcgc ctggtccggg ccacccgcag
     1981 cgacctgcgc gtgcgcgtgc ccggggcgac ctgggtgtgg ccggagaccc tcgacgccgt
     2041 gcagcccggc gaccaggtcc tgatcttcgc cgacctgccc gccggcgcgc cgatgcacgt
     2101 cgagctcggc gacaccggcg agggcgcgcg cgacgtcccg ctcaagaacg ccgagcgtcc
     2161 gctgctcgag cgcgcgtggg tcaaggccaa catcgaccgc ctctccgccg cgctcgacac
     2221 cagcgacgcc ggcaaggccg agacacgcaa acagatcctc gacctctcga tcaagtaccg
     2281 cgtgctctcc gactacaccg cgctgctggt gctcgagacc gagtacgact accagcgctt
     2341 cggcatcgac cgcagcgccc tcgccgagat cctgacggtc ggcccgtccg gcgtcaccac
     2401 gatcgaccgc agccgcctgc cgaacaaggg ccccggtttc gccggcgagg agaggtccga
     2461 ggaggagcgc agcgtgctcg acaacctgtt cggcgacgac aaggcccggg tcgccacccg
     2521 cagcaagggc gctggcgccg agccgcccgg cgcaccgacc gtcgagggcg ccaccggccg
     2581 ccccgcgccg accgaccccg cgctcgacgg tttgcccgcc ccggccgccg cgatggcccc
     2641 cggcggcgga ggcgacaccg gcatcggcgg catgcccgag cgcgacgaga gcatcggcgg
     2701 cgccccgccc cggctcgcgg cccagggcgc ccccccgccg cgcacccccg agccgatggc
     2761 cgaggccgcc aaggacgagg ccgaggagag cgacgaccgc ccgagcatgg acgccaagcg
     2821 caagtcggcc cgcccgaccg gcgcccgtcc gcagcaggac cccttcgatg tcccgacccc
     2881 gcgggtccgg ccgcggccga tcgcgccccg cccgctccag ccgaccccgc cgcccccgcc
     2941 gcggccgatc gagcgcccgc gcaagaccga cgcctacgac ggccgctacg cgatcgtcat
     3001 gtcgcaagtc gaccagcaca accccggcgg cgcgctgcgc gaggcctggc tgtggcgtga
     3061 gcagagcccc ggcgacgagc tggccctgct cgctctcggc gaggccgccg aggccagcgg
     3121 cgacgccccc ctcgccgcgc gcgcctacgg ctcgctgatc gacctgttcc ccggccgcgc
     3181 cgacatccgc cgcatggccg gcgagcggct cgaggcgctc ggcccggtcg ggctcggcct
     3241 cgccgtcgac acctacgccc aggccgtcca gcagcggccc gaccacccgt cgagccaccg
     3301 cctctacgcc ttcgccctgc tcaaggccgg ccgccacgcc gaggccttcg ccgcggcgct
     3361 caagggcgcc cagcaggcct acccctgggg ccgcttcgcc ggggtcgagc ggatcctcgc
     3421 cgacgacctg cgcctgatcg ccgccgcctg gctcgccgag cgtccgatgg atgaaaagac
     3481 gatccgcgcc gagctcggcc gggccaacgt cacccccgac accgccccct ccctgcgctt
     3541 cgtcctcaac tgggagaccg acgccaacga cgtcgacttc cacatctatg acggccaggg
     3601 cggccacgcc tactacagca accgcagcct gccctccggc ggcgagctct acgccgacgt
     3661 cacgaccggc tacgggcccg agtgcttcac cgtgccggcc caggccagcg ccttccccta
     3721 cgtgctgcag ggccactact actcgcgtgg acccatgggc tacggcatgg gcaagctcga
     3781 ggtcatcgag cacgacggcc tcggccacct gcgcttcaag gagcacccct tcgtgatcat
     3841 gaaggatggt tcattcgtcg acctgggcac gctcgacggc ccgctcgcca aggccggccc
     3901 gggcgcccgc ctggagccgg gcgaccacgg ggccgccggc aagcccggcc cggccggctc
     3961 ctacacgccc cccccgccgg tgggccagcc cctgcccccg acccgcttct aggcaaccgc
     4021 gggatcgccg gctcgcagga cctcggtgtg tggtaagttt tccaccgtag gtcctacgaa
     4081 tccatctatc cgagcaccta gagcccctgc catggcgatg aaaacactta ctctgatcaa
     4141 tcttgcctct gtcgccgcgc tggcggcctt cctcgtcggc tgcggtgaca acggcggcac
     4201 cagcgtcagc gccagcggga gcgccagcca gtcgagcacc gaccccggca gctccggccc
     4261 cgagacgccg accaccggcg ggtccggcag cctgtcggcg accgagtccg aggccggtag
     4321 tatctccgag tcgaactcga tgggcgacac cgtcaccgtc ggcggagtga gcgagggcgt
     4381 ctcgatgagc agcgaccccg gcaccaccac cggcatcggt ggcggcggct gcggcgacaa
     4441 catcctcgac cccggcgagc agtgtgacaa cggccccgac aacggccccg gccagctgtg
     4501 caagctcgac tgcaccgaca acgtctgcgg cgacaacgac cagggcccga ccgaggcctg
     4561 cgacaacggc gtcgacaacg gcgacaacgc ctcgtgcaag ctcgactgcc agccggccgt
     4621 ctgcggcgac ggcctcgtcg gccccggcga gggctgcgac gacggcaacc aggacgacgc
     4681 cgacgagtgc accaacactt gcaaggccaa ggactgcggc gacggcgtcg tcgcggcgac
     4741 cgagcagtgc gacgacgcca acgacatcga cgacgatgcc tgcaccaaca actgcaccgc
     4801 ggcgatctgc ggcgacggct tcaagcaggg cggcatcggc gaggagtgcg acgacggcaa
     4861 caacaactcc aacaccggcc agtgcaccct cggctgcctg ctcgcccagt gcggcgacgc
     4921 cttcgtgttc accgacggcg gcaacgagca gtgtgacctc ggcgccaaca acggccccgg
     4981 caagccctgc aacgcccagt gcaagatcaa cgtctgcggc gacggcgacc agaagcccga
     5041 cgaggcctgc gacgacggca acctccaggg cggcgacggc tgctcgcagt tctgcgtgct
     5101 cgaggagtgc ggcaacaaca tcgtcgaccc cggcgagcag tgcgacgacg gcaagaacgg
     5161 caacaacgcc gacagctgca ccgacttctg cctcgccccc gcctgcggcg acggcttcgt
     5221 ccaggtcaac aagggcgagg actgcgacaa cggcgcggac aacgacaaca acggcgcgtg
     5281 cacccttcag tgcaagcagg ccgactgcgg cgacggcttc gtctacatcg gcgtcgagca
     5341 gtgcgacgac ggccccaaca actcgaacac cggcaactgc aaggagaact gcagcaagca
     5401 gatctgcggc gacggcttca agggcccggg cgaggcctgc gacgacggca acctcagcaa
     5461 cttcgacagc tgcaccaacg tctgcaagca ggccggctgc ggcgacggct tcaagcagg
//
LOCUS       DASWNJ010000339         1989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_322042, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000339 DASWNJ010000000
VERSION     DASWNJ010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322042"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VGB85_05930"
     CDS             complement(<1..224)
                     /locus_tag="VGB85_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133595.1"
                     /translation="MQAETASEQPTELTKGPAIGTQAIHDARALLLDSRCVLLHERSS
                     IPLEALYTRKWFDGVTVSPYIHRVVHESGTG"
     gene            219..620
                     /locus_tag="VGB85_05935"
     CDS             219..620
                     /locus_tag="VGB85_05935"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0133596.1"
                     /translation="MHSTDAVALVEIIAAEERGEPLSQSALSQRIALTAGATSTLLSR
                     LEKRGHITRSRVKDDRRIVTLRSTPEIHRTVDQFFQPLGIRLDEMAAMYPRVVLDQIE
                     RFVVELCATMEGYIAERSQDSAGRESPEERS"
     gene            680..943
                     /locus_tag="VGB85_05940"
     CDS             680..943
                     /locus_tag="VGB85_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133597.1"
                     /translation="MAAVTADLLIKVRVFVVAVAPATGDMPQLSPVLAGDCSAAVLER
                     VTCVTDDLLASAQIAATSSESRGSEHWQERGGEGHRSRASPLV"
     gene            1097..>1989
                     /locus_tag="VGB85_05945"
     CDS             1097..>1989
                     /locus_tag="VGB85_05945"
                     /inference="COORDINATES: protein motif:HMM:NF039450.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADYC domain-containing protein"
                     /protein_id="HEY0133598.1"
                     /translation="MTWNSKQTACAAALLAMVGCDAQVDSRGGDGVPPLSPQGEVNGV
                     LLNSFRLNSFRLNSFRLNSFRLNGDPGTGDYIELADIDLPGKAEAEYSSVQGSTLYIK
                     TTDGDILGRDELTGTVFSFTVQENDVKIDREVRITGVRQLTDEAETTKKKKKYKKQDW
                     DDAGSDITLYDLEIREGGKGKWEPLCVDERGEASEAVLLTDIWDPETGDRVTPRPTDA
                     VTFACVDAALGKCAVWGYRPWAEHDGEPLADYHQACTRLVRADYCGNGVSYTAAGTPI
                     HVLDQLGIEVKALDITYAVEA"
BASE COUNT          357 a          605 c          697 g          330 t
ORIGIN      
        1 ccggtgccgg attcgtgaac gacccgatga atatacggtg aaacagttac accgtcaaac
       61 cattttctcg tgtaaagtgc ctccaagggg atggaggagc gctcgtgaag gaggacacag
      121 cggctgtcga gcaacagcgc gcgcgcatca tggatggcct gcgtgcctat ggcagggcct
      181 ttggtgagct cggtcggctg ttcgctggcg gtctcggctt gcacagcacg gatgcggtcg
      241 cgctcgtcga gatcatcgcg gccgaggagc gtggcgagcc gctgtcgcag tcggcgctga
      301 gccagcgcat cgcgctgacg gccggtgcca cctcgacctt gttgagccgc ctcgagaagc
      361 gcggccacat cacgcgctcc cgcgtgaagg atgaccggcg gatcgtcacc ctgcgctcga
      421 ctccggagat tcatcggaca gtggaccaat tttttcaacc gctgggtatt cgcctcgacg
      481 agatggcggc gatgtacccc cgcgtggtgc tcgaccagat cgagcgcttc gtcgtcgagc
      541 tgtgcgcgac gatggagggc tacatcgcgg agcgctcgca ggacagcgcg gggcgcgaat
      601 ctccggagga gcggtcgtga cggcggcgct gcgtgcatat cgccggtagc gccgtcatcg
      661 ccggcataaa atcatcgcat tggccgcggt caccgccgat ttgctgatca aagtgcgcgt
      721 atttgtcgtc gcggtcgcgc cggcgacggg cgatatgccg caattgtctc cggttctggc
      781 cggcgattgc agcgcagcgg tcctggagag ggtgacgtgc gtcaccgatg atttgctcgc
      841 aagcgcgcaa atagccgcga cttcaagcga atcccgggga tccgaacatt ggcaggagcg
      901 tgggggcgag gggcatcggt cgcgcgcatc gccattggtt tgatcagtat cgtcaactcc
      961 tgttcctgtg aaagcccgcg tggcgtcgct gcatatgcgc acacgaggtt cgatcaggtc
     1021 agctgtccgg gctgggccga cgtcggaggc tgacaccgac agcgtgctag tctcggatcg
     1081 aaacgggagg cgagtgatga cctggaacag caagcaaacg gcgtgtgcgg cggctctact
     1141 ggcgatggtg ggttgcgacg cgcaggtcga ctccaggggg ggcgacgggg tgccgccgct
     1201 gtccccgcag ggtgaagtga acggggtgtt gctcaactcg ttccggctca actcgttccg
     1261 gctcaattcg ttccggctca actcgttccg gctcaacggt gatccgggga ccggcgatta
     1321 catcgagctg gcggacatcg acctgccggg caaggccgag gcggagtaca gctcggtgca
     1381 gggcagcacg ctttatatca agaccaccga cggcgacatc ctcggccgcg acgagctcac
     1441 gggcacggtc ttttcgttca cggtccagga gaacgacgtc aagatcgacc gcgaggtgcg
     1501 gatcaccggc gtgcgtcagc tgaccgacga ggccgagacc accaagaaga agaagaagta
     1561 caagaagcag gactgggacg acgccggctc ggacatcacg ctctacgacc tcgagatccg
     1621 cgagggcggc aagggcaagt gggagccgct gtgcgtcgac gagcggggcg aggcgagcga
     1681 ggccgtcctg ctgacggaca tctgggaccc ggagaccggc gaccgcgtga ctccgcgccc
     1741 gaccgacgcg gtcaccttcg cgtgcgtcga cgcggcgctc ggcaagtgcg ccgtgtgggg
     1801 ttaccgcccg tgggcggagc atgacggcga gccgctggcg gactaccacc aggcgtgcac
     1861 gcgcctggtc cgcgccgact actgcggcaa cggcgtctcg tacacggccg ccggcacgcc
     1921 gatccacgtg ctcgatcagc tcgggatcga ggtcaaggcc ctcgacatca cctacgcggt
     1981 cgaggcaga
//
LOCUS       DASWNJ010000340         1609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_500416, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000340 DASWNJ010000000
VERSION     DASWNJ010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500416"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1609
                     /locus_tag="VGB85_05950"
     CDS             <1..>1609
                     /locus_tag="VGB85_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEY0133599.1"
                     /translation="DDLARHGPVTHGKESARVDDAAFNLFVRSGRGGDANLAYAEKRL
                     DMNASRMKVRQLLHLYRKLLDNDRVLAEPNNQIQTELRLTGLAAEWRAEGGEIYLRTR
                     NLIFAEVYDHKWLKLKEKEQRLAELVDRWSSSGKLDDYLLRGAALEDALAWSHGRSDL
                     TVIDHEFLLAGVNYARREAENRHKLEETARKEEAAGRKAIEEQRKADRERYRADIERE
                     RRERAEEGALSQRRTISILTGTVALLGVLLALFAWQYIVAERSKTLLADLDDRNREDA
                     HLQQLGAEALLLSQQAGKERQALRIAIGAASKSMQQGRSVPPRVMQGLAAAVATPVQR
                     PVVLRHGEEITAADFSHDGTRVLTASRDHTAHLWDAVSGRSIRVLGEHGGAVLAASFS
                     IDDGRIFTVSADRQARLWNAASGEVLDTYKGLVNPQPQRFLAAMAPDATRVLLSQSDR
                     TLRLAELRNNKTIDTLRGHTAAITTAAFSPDGLRLVTACADGSARLWSAETGRAVAEM
                     RRHAGALRSVAFSPNGARIVTVGEDRTA"
BASE COUNT          257 a          600 c          560 g          192 t
ORIGIN      
        1 cgacgacctc gcccgccacg gcccggtcac gcatggaaag gagtccgcgc gggtcgacga
       61 tgccgccttc aatttgttcg tccgaagcgg ccgcggcggc gacgccaacc tcgcctacgc
      121 cgagaagcgg ctcgacatga acgcctcgcg gatgaaggtg cgccagctgc tgcacctcta
      181 ccgcaagctg ctcgacaacg accgcgtgct cgccgagccg aacaaccaga tccagaccga
      241 gctgcggctg accgggctcg ccgccgagtg gcgcgccgag ggcggagaaa tttatctgcg
      301 cacgcgcaac ctgatcttcg ccgaggtcta cgaccacaag tggctcaagc tcaaggagaa
      361 ggagcagcgc ctcgccgagc tggtcgaccg ctggtcgagc tcgggcaagc tcgacgacta
      421 cctgctgcgc ggcgcggccc tcgaggacgc gctcgcgtgg tcgcacggcc gcagcgacct
      481 gaccgtcatc gaccacgagt tcttgctcgc cggcgtcaac tatgcccgcc gcgaggccga
      541 gaaccggcac aagctcgagg agacggcgcg caaggaggag gccgccggtc gcaaggcgat
      601 cgaggagcag cgcaaggcgg accgcgagcg ctaccgggcc gacatcgagc gcgagcggag
      661 agagcgggcc gaggagggcg ccctgtcgca gcggcgcacg atcagcatcc tgaccggcac
      721 cgtcgcgctg ctcggcgtgc tgctcgcgct gttcgcctgg cagtacatcg tcgccgagcg
      781 cagcaagacc ttgctcgccg acctcgacga ccgcaaccgc gaggacgcgc acctgcagca
      841 gctcggcgcc gaggcgctgc tgctctcgca gcaggccggc aaggagcgac aggcgctgcg
      901 catcgcgatc ggcgcggcca gcaagagcat gcagcagggg cgctcggtcc cgccgcgggt
      961 gatgcagggg ctcgcggccg cggtcgccac gcccgtgcag cggccggtgg tgctgcgcca
     1021 cggcgaggag atcacggccg ccgacttctc gcacgacggc acccgcgtac tgacggccag
     1081 ccgcgaccac accgcgcacc tgtgggacgc cgtgtcgggc aggtcgatcc gcgtgctcgg
     1141 tgagcacggc ggggccgtgc tcgcggccag cttctcgatc gacgacggcc gcatcttcac
     1201 ggtctcggcc gaccgccagg cccggctgtg gaacgccgcc tcgggcgagg tgctcgacac
     1261 ctacaagggc ctggtcaacc cgcagccgca gcgcttcctg gcggcgatgg cccccgacgc
     1321 gacccgcgta ttgctgtcgc agagcgaccg gactttgcgc ctcgccgagc tgcgcaacaa
     1381 caagacgatc gacaccctgc gcggccacac cgccgcgatc acgacggccg cgttctcgcc
     1441 cgacggcctg cgcctggtca ccgcgtgcgc cgacggcagc gcccgcctgt ggtcggccga
     1501 gaccgggcgc gccgtcgccg agatgcgccg ccacgccggc gcgctgcgct cggtcgcctt
     1561 ctcgcccaac ggcgcgcgca tcgtcaccgt cggcgaggac cgcacggcc
//
LOCUS       DASWNJ010000341         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_500418, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000341 DASWNJ010000000
VERSION     DASWNJ010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500418"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1611
                     /locus_tag="VGB85_05955"
     CDS             <1..1611
                     /locus_tag="VGB85_05955"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEY0133600.1"
                     /translation="LEHESLRGRERDFLTASLADLRRQRRVKRIALVAAPLLLFAVFI
                     GFRVQQTRELAGKVEQQLARAEASLAEARAAQDQAVRLRDQAFAEFDARRSTAEARWK
                     LARAAVQAADRRYRATLQALETVLGLDFEHTGARARLGEAIFERAVAAEASGKLELAA
                     ELIERLPQDSPQLARWGAPGRLSLATDPPSAVTLAEYRLDPDTLRRELGPARALGDTP
                     VRGLVLAQGSYRLGLRVAGGDEVVYPLLVARAQQIDLDLRLPRPGQLPAGFVFVPAGQ
                     SPIGSADEEMIRNFFVTVPQHPVRLPAYVIARHETTYAQWLAFLRDLPPDERARYLPS
                     SETMLLGAGIILAQDADGRWSLSMSAGERTLHAVEGEPIAYPGRTAHATQDWLQMPVG
                     GINFADAVAYLAWLDRSGRVPGARLCSDWEWERAARGVDGRVYPHGDRLAPGEANIDR
                     TYGDAAENRGPDMVGLHPESRSPFGVDDMTGNNWEWVRSSVEDGGGVVRGGAFAYEET
                     TAASLNRNRLPLETRDSTVGLRVCASIQ"
     gene            1809..>2062
                     /locus_tag="VGB85_05960"
     CDS             1809..>2062
                     /locus_tag="VGB85_05960"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEY0133601.1"
                     /translation="MEQGEVVDDDHIDFTPVDDAITFRGVAENGMESNGAKLNGAKLN
                     GAKLNGAKLNGVALNGAERLGATISGLSFTGGSLLSAFDQG"
BASE COUNT          297 a          758 c          722 g          285 t
ORIGIN      
        1 ctcgagcacg agtccctgcg cgggcgcgag cgcgacttcc tgacggccag cctcgccgac
       61 ctccgacgcc agcgccgcgt caagcggatc gccctcgtcg ccgcgccgct gctgctgttc
      121 gccgtgttca tcggctttcg cgtgcaacag acgcgcgagc tcgccggcaa ggtcgagcag
      181 cagctcgcgc gcgccgaggc ctccctcgcc gaggcccgcg ccgcgcagga ccaggccgta
      241 cgcctgcgcg accaggcctt cgcggagttc gacgccaggc gcagcaccgc cgaggcgcgc
      301 tggaagctcg cgcgcgccgc ggtgcaggcg gcggaccgtc gctaccgggc cactttgcag
      361 gcgctggaga ccgtgctcgg cctcgacttc gagcacaccg gggcccgggc ccgcctcggc
      421 gaggcgatct tcgagcgcgc ggtcgcggcc gaggcgagcg gcaagctcga gctggcggcc
      481 gagctgatcg agcggctgcc gcaggacagc ccgcagctgg cccgctgggg cgccccgggt
      541 cgcctgtcgc tcgccaccga cccgccgagc gccgtgaccc tcgcggagta ccggctcgac
      601 ccggacacgc tgcggcgcga gctcggccct gcgcgtgcgc tcggcgacac gccggtccgc
      661 ggcctcgtgc tggcgcaggg cagctatcgc ctcggcttgc gggtcgccgg tggggacgag
      721 gtcgtttatc ccttgctcgt cgcgcgcgcc cagcagatcg acctcgacct tcgcctgccg
      781 cgcccgggcc agctgcccgc cggcttcgtg ttcgtgccgg cggggcagtc cccgatcggc
      841 agcgcggacg aggagatgat ccgcaacttc ttcgtcaccg tcccgcagca cccggtccgc
      901 ctgcccgcgt acgtcatcgc ccgccacgag accacctacg cgcagtggct cgccttcctg
      961 cgcgacttgc cccccgacga gcgcgcgcgc tacctgccga gcagcgagac catgctgctc
     1021 ggcgccggca tcatcctcgc gcaggacgcc gacgggcgct ggtcgctctc gatgtccgcc
     1081 ggcgagcgca ccctccacgc cgtcgagggc gagccgatcg cctacccggg gcgcaccgcc
     1141 cacgcgacgc aggactggct gcagatgccg gtcgggggca tcaacttcgc cgatgcggtc
     1201 gcctacctcg cctggctcga ccgcagcggt cgcgtgcccg gggcgcgcct gtgcagcgac
     1261 tgggagtggg agcgagccgc ccgcggcgtc gacgggcgcg tctatcccca cggcgaccgg
     1321 ctcgcccccg gagaggccaa catcgaccgc acctacggcg acgccgcgga gaaccgcggc
     1381 ccggacatgg tcggcctcca ccccgagtcg cgcagcccgt tcggcgtcga cgacatgacc
     1441 ggcaacaact gggaatgggt gcgatcgtcc gtcgaggacg gcggtggcgt cgtgcgcggc
     1501 ggagcgttcg cctacgagga gacgaccgcc gcctcgctca accgcaaccg cctacccctg
     1561 gagacacgcg actccacggt gggcctccgc gtctgcgctt cgatccagtg aaggcgcggc
     1621 ggtcgagtga ccatgcagga aatgccggtc ggtttccgat tgaggcacga aacaatgtgg
     1681 gatagagaga cgtgggggtt tgtatgacca caaaatggca aatgggttgg gtttctgcgg
     1741 ttagttcttg cttatcgacg ctcgcaatcg ctggatgcga cgcgcccgaa gatctgaacg
     1801 tcggggtatt ggagcagggt gaggtcgtcg acgacgacca catcgacttc accccggtcg
     1861 acgacgcgat cacgtttcgt ggcgtcgcgg agaacgggat ggagagcaac ggggccaagc
     1921 tcaacggcgc caagctcaac ggggccaagc tcaacggcgc caagctcaac ggcgtcgcgc
     1981 tcaacggcgc cgagcggctc ggcgcgacga tctccgggct ctcgttcacc ggcgggagcc
     2041 tgttgagcgc cttcgaccag gg
//
LOCUS       DASWNJ010000342         1573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_500489, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000342 DASWNJ010000000
VERSION     DASWNJ010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500489"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..1015)
                     /gene="mvaD"
                     /locus_tag="VGB85_05965"
     CDS             complement(26..1015)
                     /gene="mvaD"
                     /locus_tag="VGB85_05965"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEY0133602.1"
                     /translation="MRAARAVAHTNIALIKYWGKRPGDPDQNLPAVGSLSLTLERLRS
                     DTTVRASEQDSFVLDGADDIGAAAKVFRHLDRVWRTANPGTRPPCAVTSTNHLPTAAG
                     LASSASGFAALTLAAAGAFGLDLDDRGALSALARRGSGSAARSLWGGFVRQLRGELPD
                     GSDCRARPLFGAEHWDLRLLIVHTAKGKKPVGSTDGMQRSADTSPYYPAWVATSESDL
                     ARGEQALAARDLEALGAVMEHSCFKMHSCMLATVPPLMYWNGTTLAAIQEVWAARRDG
                     LEGYVTSDAGPHVKVLCRPDVADRLAARLAAVPGVHTVDTLGPGPDAAVELLA"
     gene            complement(1104..>1573)
                     /locus_tag="VGB85_05970"
     CDS             complement(1104..>1573)
                     /locus_tag="VGB85_05970"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133603.1"
                     /translation="AAQGGVLRFVRGEGAEPVILGGPLPLVLIPSGIPRSTATQVGKV
                     RARLERHPTILRPTLSALAAAAEAGIEALARHDLDALGEILNIAHSLLGAIDVSSPAL
                     DALQVTALAAGARGAKLTGAGGGGCLLVLPPADPAPLIAAFEIYQPLSVEVSA"
BASE COUNT          214 a          489 c          680 g          190 t
ORIGIN      
        1 gagggacagg tgcgcgggga catggtcatg cgaggagctc gacggcagcg tcggggccgg
       61 ggccgagggt gtcgacggtg tgcacgcccg ggaccgcggc gaggcgggcg gcgaggcggt
      121 cggcgacgtc ggggcggcac aggaccttga cgtgcgggcc ggcgtcgctg gtgacgtagc
      181 cctcgaggcc gtcacgacgg gccgcccaga cctcctggat cgcggcgagc gtggtgccgt
      241 tccagtacat cagcggcggc acggtggcga gcatgcagct gtgcatcttg aagcagctgt
      301 gctccatcac ggcgccgagc gcctcgaggt cgcgggcggc gagggcctgc tcgccgcgcg
      361 cgaggtcgga ctcggaggtg gcgacccacg cgggatagta gggggaggtg tcggcgctgc
      421 gctgcatgcc gtcggtcgag ccgaccggct ttttgccctt cgcggtgtgc acgatcagca
      481 ggcgcaggtc ccagtgctcg gcgccgaaca gcggccgcgc gcggcagtcg gagccgtcgg
      541 ggagctcgcc acgcagctgg cggacgaagc cgccccacag cgagcgcgcg gcggacccgg
      601 agccgcgccg cgcgagcgcc gagagggccc cgcgatcgtc gaggtcgagg ccgaaggcgc
      661 cggcggccgc gagcgtcagc gcggcgaagc ccgaggcgga ggaggcgagg ccggcggcgg
      721 tcgggagatg gttggtcgag gtgacggcgc agggcgggcg ggtgccggga tttgcggtgc
      781 gccacacgcg gtcgaggtga cgaaacacct tggccgccgc gccgatatcg tcggcgccgt
      841 cgagcacgaa gctgtcctgc tccgaagcac gtacggtggt gtcgctgcgc aggcgctcga
      901 gcgtcagcga cagcgagccg accgcgggca ggttctggtc gggatcgccg gggcgcttcc
      961 cccagtactt gatcagggcg atgttggtgt gcgcgacggc gcgggcggct ctcacggggt
     1021 cacctctcga acgtgctgcg ggctcgcggc gagcgtggtg cggatcgtcg cacgcgcgca
     1081 cctgtccttc gggacagcga gggtcaagcg ctcacctcga cggagagcgg ctgataaatt
     1141 tcgaaggcgg cgatcagcgg cgcggggtcg gccgggggga gcacgagcag gcagccgcca
     1201 ccgccggcgc cggtgagctt ggccccgcgg gcgccggcgg cgagggcggt gacctggagg
     1261 gcgtcgagcg cgggagagga gacgtcgatc gcgccgagga gactatgggc gatgttgagg
     1321 atctcgccga gggcgtcgag gtcgtggcgg gcgagggcct cgatgccggc ctcggccgcg
     1381 gcggcgaggg cggacagcgt cgggcggagg atggtcgggt ggcgctcgag gcgggcgcgg
     1441 accttgccga cctgggtggc ggtcgatcgt gggatgccgc tcgggatcag gaccagcggg
     1501 agcgggccgc cgaggatgac gggctcggca ccctcgccgc ggacgaagcg aagcacgccg
     1561 ccctgcgcgg cga
//
LOCUS       DASWNJ010000343         4322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_322124, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000343 DASWNJ010000000
VERSION     DASWNJ010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322124"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VGB85_05975"
     CDS             <1..556
                     /locus_tag="VGB85_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEY0133604.1"
                     /translation="LLGLWAIYDALEAGLQRHAGHPVAGPLALPELYRSPAIAADLGD
                     LFDLTPGARPIVPAAVAYAERLYRLADAAPSLLVAHAYTRYLGDLSGGQILRLGAARV
                     LGLPAEAPHGTPGLAFYDFPIDDIGAYKQLYRARLDALPVDAGQAAAIIGEARWAFAG
                     TAAVFGELLPDSASFMPHAASHAR"
     gene            complement(585..2030)
                     /locus_tag="VGB85_05980"
     CDS             complement(585..2030)
                     /locus_tag="VGB85_05980"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEY0133605.1"
                     /translation="MTRPSNILTRRHMLQGLGLSSLMGIAGLTGLGTITRSRPAVAAP
                     GDIPRRIIFFYGSGMLYDFWEPTASVGKPTPEEYDFQLGELHAPLQPFKNRMIYLDGI
                     GMVSEEVDPGEKGNAHNQGAKHALAPYDSAGTDLPGGASIDQFIAKALNEPQAVTPHA
                     SLILQAAGWTNELTLFSGAVASGPGALVPGIWDPRAAYDKVFGNFQGGDDPAVIDKLR
                     AQRAAVFGLAADDFGGLQSRVGTADAERLQIHLDLLRDLEMRLELQDTLTCTPPPAFT
                     TPPGNFEKYDASYFAGNYNLSADLHSELITAALACDQTRVVTFEHQYPGDADFGYAAG
                     QFDTTDAHDLVHKVNDKNSPQYQIPEAVACIRKMCTLEAQKLANLLTMLGKVPEADGK
                     TLLDHTLVLYCGQIGYGSHDLARLPWVVIGDAGGFFKTGRYIKYPANPNSTRGLPHNN
                     LYVSLANAMGIPIDTFGNPAACTGPLAGLTG"
     gene            complement(2032..3579)
                     /locus_tag="VGB85_05985"
     CDS             complement(2032..3579)
                     /locus_tag="VGB85_05985"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEY0133606.1"
                     /translation="MTTGGEPASCEPLRSPLRRLSRTELANTLADLFPTVTLPEIDLP
                     ADKPKDLFGNSVDTQEPTDVYVETHWRATVTVAAAVAADLPAVMGCSLADDAARQACA
                     DTYVPLLLRRAFRRPVTQAQVDRYLADFLPTQWGEHGFDTAMQLLVTAVLNSPSFLYR
                     AEFGADGADASATMPLRAEELAARLSYFLWQTMPDDTLAAAAESGVLNTPAGLEAEAR
                     RMLADPRARVAIADFHRQWLGLDGLQAVERDEALFPAWKPELAPLMAESLRQYVAYAF
                     WDGEGTLASLLLDPIAFANAALAPIYGVQAEGEALGLVSLDGAQRAGLLTQPALMASM
                     AHEKFDAPIKRGVYVLDRFMCVGLGAPPPDVPDIPPADANEGPKTTRQRIEETHVQES
                     CKSCHTLIDGVGFAFNNYDAVGQWRVTENGLPIDATGALMGTAGITANFDGALVLSQL
                     LADSPEVERCFGRQVFRFAQGRREGAGDLCALDEALAAAGGDMRELMIQLVLTDNFRQ
                     RAAIEGE"
     gene            complement(3519..>4322)
                     /locus_tag="VGB85_05990"
     CDS             complement(3519..>4322)
                     /locus_tag="VGB85_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133607.1"
                     /translation="EYFPHGDGVYIVTGESGDSRTIVRSPDGITWAPASQRPPECGTY
                     AYGMAYGNDTIVLASGKGGICRSQDGGDTWEHTPVTEQFSSPPVWTGAEFYIYDGATL
                     WHSADGAEWTSEGVTPDSVRIGALARSPEGTLVAANAGWMTWYEKQQFYRSTDGKTWE
                     VLAPGTFVGSHPINFISHGTVSPGAGCPAPCPCDPPRFYSRWRSPAVTTARAPTAPTA
                     WTPEVRPRRPRTRPPGPTTTARRPAVTPTTSPPAASRPAASRCARRCVA"
BASE COUNT          558 a         1438 c         1654 g          672 t
ORIGIN      
        1 cctgctcggg ttgtgggcca tctacgacgc gctcgaggcc ggcttgcagc gccacgccgg
       61 tcacccggtc gctggcccgc tcgcgctgcc cgagctgtac cgcagcccgg cgatcgccgc
      121 cgacctcggc gacctgttcg acctcacgcc tggcgcccgg ccgatcgtgc ccgccgcggt
      181 cgcctacgcc gagcgcctgt atcgcctcgc cgacgccgcg ccctcgctgc tggtcgccca
      241 cgcgtacacg cgctacctcg gcgacctctc gggcgggcag atcctgcggc tcggcgccgc
      301 gcgggtgctc gggctcccgg ccgaggcgcc gcacggcacg cccggcctcg ccttctacga
      361 cttcccgatc gacgacatcg gggcctacaa gcagctctac cgggcccgcc tcgacgccct
      421 gccggtcgac gccgggcaag ccgccgcgat catcggcgag gcgcgctggg ccttcgcggg
      481 cacggcggcc gtgttcggcg agctgctccc ggacagcgcg agcttcatgc cgcacgccgc
      541 gagccacgcg cgctgacctg cgcgcgtcac ggcgcccggg gtcgctagcc ggtgagcccc
      601 gcgagcggcc cggtgcaggc ggcgggatta ccaaacgtat cgatcgggat gcccatcgcg
      661 ttggcgagcg acacgtagag gttgttgtgc ggcagcccgc gggtcgaatt cgggttggcc
      721 gggtacttga tgtaacggcc cgtcttaaaa aatccgccgg cgtcgccgat gacgacccac
      781 ggcagccgtg cgaggtcgtg gctgccgtag ccgatctgcc cgcagtagag gacgagggta
      841 tgatcgagca gcgtcttgcc gtcggcctcc ggcaccttgc cgagcatcgt cagcaggttg
      901 gcgagctttt gcgcctcgag cgtacacatc ttgcggatgc acgcgacggc ctcggggatc
      961 tggtattgcg gcgagttctt gtcgttgacc ttgtgcacga ggtcgtgcgc gtcggtggtg
     1021 tcgaactggc ccgccgcgta gccgaagtcg gcgtcgcccg ggtactgatg ctcgaaggtg
     1081 acgacgcgcg tctgatcgca ggcgagcgcg gcggtgatca gctcgctgtg caggtcggcg
     1141 ctgaggttgt aattgccggc gaagtagctc gcgtcgtact tttcgaagtt gcccggcggc
     1201 gtcgtgaagg ccggcggggg cgtgcaggtc agcgtgtcct gcagctcgag gcgcatctcg
     1261 aggtcgcgca gcaggtcgag gtggatctgc aggcgctcgg cgtcggccgt gccgacgcgg
     1321 gactgcaggc cgccgaagtc gtcggcggcg aggccgaaca ccgcggctcg ctgggcgcgc
     1381 agcttgtcga tcacggcagg atcgtcgccg ccctggaagt tgccgaacac cttgtcgtag
     1441 gcggcgcgcg ggtcccagat gccgggcacg agcgcaccgg ggccgctggc gaccgcgccg
     1501 gagaacaggg tcagctcgtt ggtccagccg gcagcttgca ggatcagcga ggcgtgcggc
     1561 gtgacggcct ggggttcatt gagcgccttg gcgatgaact gatcgatcga cgcgccgccg
     1621 ggcaggtcgg tgccggcgct gtcgtagggc gcgagcgcgt gcttggcccc ctgattgtgg
     1681 gcgttgccct tctcgccggg gtcgacctcc tccgagacca tgccgatccc gtcgaggtag
     1741 atcatgcgat ttttaaatgg ttgcagcggc gcgtgcagct cgccgagctg gaagtcgtac
     1801 tcctcgggcg tcggcttgcc gacgctcgcg gtcggctccc agaagtcgta cagcatgccc
     1861 gagccgtaga agaagatgat gcggcgcggg atgtcgcccg gggcggcgac ggcgggccga
     1921 ctgcgggtga tggtaccgag gccggtcagg ccggcgatgc ccatgaggct ggagagcccg
     1981 aggccttgca gcatgtgacg gcgggtgagg atgttggatg ggcgggtcat gtcactcccc
     2041 ctcgatcgcg gcgcgctggc ggaagttgtc ggtgagcacg agctggatca tcagctcgcg
     2101 catgtcgccg ccggcggcgg cgagcgcctc gtcgagcgcg cacaggtccc cggcgccctc
     2161 gcggcggccc tgggcgaagc ggaacacctg gcggccgaag cagcgctcga cctccgggct
     2221 gtcggcgagc agctgcgaga gcacgagcgc gccgtcgaag ttggcggtga tgccggcggt
     2281 gcccatcagc gcgccggtgg cgtcgatcgg caggccgttc tcggtgacgc gccactgacc
     2341 gaccgcgtcg tagttgttga acgcgaagcc gacgccgtcg atcagcgtgt ggcagctctt
     2401 gcacgactcc tgcacgtggg tctcttcgat ccgctggcgg gtcgtcttcg ggccctcgtt
     2461 ggcgtcggcg ggcgggatgt cgggcacgtc cggcggcggc gcgccgaggc cgacgcacat
     2521 gaagcggtcg agcacgtaga cgccgcgctt gatcggcgcg tcgaacttct cgtgggccat
     2581 gctcgccatc agggccggct gggtcagcag accggcccgc tgcgcgccgt cgagggagac
     2641 gaggccgagc gcctcgccct cggcctgcac gccgtagatc ggcgcgagcg cggcgttggc
     2701 gaaggcgatc gggtcgagca gcaggctggc gagcgtgccc tcgccgtccc agaaggcgta
     2761 ggcgacgtac tggcgcagcg actcggccat caggggggcg agctccggct tccacgccgg
     2821 gaacagcgcc tcgtcgcgct cgaccgcctg caggccgtcg aggccgagcc actggcggtg
     2881 aaagtcggcg atagcgacgc gggcgcgcgg gtcggcgagc atgcgccgcg cctcggcctc
     2941 gaggcccgca ggcgtgttga gcacgcccga ctcggccgcg gccgcgaggg tgtcgtcggg
     3001 catcgtctgc cacaggaagt acgacaggcg cgccgcgagc tcctcggcgc gcagcggcat
     3061 ggtcgcgctc gcgtcggcgc cgtcggcccc gaattcggcg cgatacagga agctcggcga
     3121 attgagcacg gcggtgacga gcagctgcat cgcggtgtcg aagccgtgct cgccccactg
     3181 ggtcggcagg aagtcggcga ggtagcggtc gacctgggcc tgggtgaccg ggcggcgaaa
     3241 cgcgcggcgc agcagcagcg gcacgtaggt gtcggcgcag gcctggcgcg cggcgtcgtc
     3301 ggcgagcgag cagcccatga cggcgggcag gtcggcggcg acggccgcgg cgacggtgac
     3361 ggtcgcgcgc cagtgggtct cgacgtagac gtcggtcggc tcctgggtgt cgacgctgtt
     3421 gccgaacagg tccttgggct tgtcggcggg caggtcgatc tccggcagcg tgacggtcgg
     3481 gaacaggtcg gcgagggtgt tggcgagctc ggtgcggctc aggcgacgca gcggcgagcg
     3541 cagcggctcg cagctggccg gctcgccgcc ggtggtgatg tcgtcggtgt caccgccggt
     3601 cggcgtgccg tcgtcgtcgg acccgggggt cgggtccgcg gtcgccgcgg tcgaacctcc
     3661 ggtgtccacg ctgtcggcgc tgtcggtgct cgcgccgtcg taacagccgg cgatcgccag
     3721 cgcgagtaaa agcggggtgg gtcgcatggg catggcgcgg ggcatccggc tccgggggac
     3781 acggtcccgt ggctgatgaa gttgatcggg tggctgccga cgaaggtgcc cggcgcgagc
     3841 acctcccagg tcttgccgtc ggtgctgcga tagaactgct gcttctcgta ccaggtcatc
     3901 cagccggcgt tggcggccac cagcgtgccc tccgggctgc gcgcgagcgc gccgatgcgg
     3961 acgctgtcgg gcgtgacgcc ctcgctcgtc cactcggcac cgtcggcgct gtgccacagc
     4021 gtcgcgccgt cgtagatgta gaactcggcg ccggtccaca ccggcggcga cgagaactgc
     4081 tcggtgacgg gggtgtgctc ccaggtgtcg ccaccgtcct gcgagcgaca gatgccgccc
     4141 ttgccgctgg cgaggacgat cgtgtcgttg ccgtacgcca tgccgtaggc ataggtgccg
     4201 cactcgggcg gtcgctggct cgcgggcgcc caggtgatgc cgtcggggct gcgcacgatc
     4261 gtgcggctgt cgccgctctc gccggtgacg atgtacacgc cgtcgccgtg cgggaagtac
     4321 tc
//
LOCUS       DASWNJ010000344         2944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_289204, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000344 DASWNJ010000000
VERSION     DASWNJ010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289204"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /gene="mtnA"
                     /locus_tag="VGB85_05995"
     CDS             <1..897
                     /gene="mtnA"
                     /locus_tag="VGB85_05995"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEY0133608.1"
                     /translation="AIETLAVRGAPAIGCTAALGLAAASTGFSDDPTLFVEACGRAID
                     RLAHTRPTAVNLFHALGHMRLALASASGDVTSRRTTLRAAAEAYVDAELQACLRMGEF
                     GAPLLPEGTILTHCNTGALATAGWGTALGVIRRAHQIGKAIRVLADETRPVLQGARLT
                     AWELQRDGIPVEVIADNMAGALMHRGQIQAAIVGADRITRRGDVANKIGTYTVAVLCR
                     HHGIPFFVAAPWSTIDLEMESGAQIEIEQRNPDEVHFHGSTRMTPAGVGARNPAFDVT
                     PAELITAIITERGVFAPHELVR"
     gene            complement(958..2445)
                     /locus_tag="VGB85_06000"
     CDS             complement(958..2445)
                     /locus_tag="VGB85_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0133609.1"
                     /translation="MTTIVTQAHARVMSTPKPRSPLFVGRYHVLRELGRGGMGTVHLA
                     YQPELGREVALKRILPENVGLDSAEGWFKREYTALASLRHPGIPAIYDCGRSDDGVAY
                     FTMEIIEGPSLASALKARKFEAVEAITIAIDLARILAVTHAVGVIHRDVKPTNIILEA
                     GGLVRLIDFGICFLLPRFRGRPALRTVGEDEYRTGPMEVAGSPGYTDPALMNGHPPSV
                     QSDIFSVSVILYEMLARRRLYDDKTGSFRQIDTGEFAPELAPVVTELQCGAQLLPRDR
                     HASMDQFVRDLEIARSAVIRAQSAAPTSSGRGLLLAFSVINSIVLLAVLGFFAAGRPG
                     LDGPVSPPIGSAVPTTQVRSPVEPAAIGSALAEPAAADLAPVELAAPKSAVAGPPADE
                     RPRLPLGPTSRVTPGTDESRVLSQALVSSLAMPALPALRACQHEPGPSNLQIAVKAGR
                     ARLVQVEWVAYDRKDPTHRCFLRALGTIDFPRQGPVGPYRLRFVK"
     gene            2511..>2944
                     /locus_tag="VGB85_06005"
     CDS             2511..>2944
                     /locus_tag="VGB85_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133610.1"
                     /translation="MSALVWLTAISLAAPLVTPVSTSPPPDVIDEALEFYDQGAFLEA
                     AAVAIDGYVSPARPPAERLELARLAQESFSRAYEVSTASTSRPEYLCRALDVLDATAP
                     LAITAKDRERHEKLRATHQAKLAERHPDFVCEVGTEFRPIVVT"
BASE COUNT          472 a         1011 c         1015 g          446 t
ORIGIN      
        1 gcgattgaga cgctggccgt gcgcggcgcc ccggcgatcg gctgcaccgc ggcgctcggg
       61 ctcgccgccg cgagcaccgg cttctccgac gatccgacgc tgttcgtcga ggcctgcggg
      121 cgagcgatcg accgcctcgc ccacacgcgc ccgacggcgg tcaacctgtt ccacgcgctc
      181 ggccacatgc gcctcgcgct cgccagcgcg tccggtgatg tgacgagccg gcgcacgacg
      241 ctgcgcgccg cggccgaggc ctacgtcgac gccgagctgc aggcctgcct gcgcatgggt
      301 gaattcgggg cgccgctgct gcccgagggc acgatcctca cccactgcaa caccggcgcg
      361 ctggcgaccg ccggctgggg caccgcgctc ggcgtcatcc gccgcgcgca ccagatcggc
      421 aaggcgatcc gcgtgctcgc cgacgagacg cgcccggtgc tgcaaggcgc ccgcctgacc
      481 gcgtgggagc tgcaacgtga cggcatcccg gtcgaggtga tcgccgacaa catggccggc
      541 gccctgatgc atcgcggcca gatccaggcg gcgatcgtcg gcgccgaccg catcacccgc
      601 cgcggcgacg tcgccaacaa gatcggcacg tacacggtcg cggtgctgtg ccgccaccac
      661 ggcatcccgt tcttcgtcgc cgccccgtgg tcgacgatcg acctcgagat ggagtccggc
      721 gcgcagatcg agatcgaaca gcgcaacccc gacgaggtcc actttcacgg gagcacgcgc
      781 atgacgcccg ccggcgtcgg cgctcgcaac cccgcgttcg acgtgacgcc ggccgagctg
      841 atcacggcga tcatcacgga gcgcggggtg ttcgcgccgc acgagctcgt gcgctgagag
      901 cacgcgtcgg gatcgtcgca tgcccgggcc cggcgcgtcg agccggcaca gtgataacta
      961 ttttacgaac cggagccgat aggggccaac gggtccttgt cgtgggaagt ctatcgtgcc
     1021 gagcgcgcgc agaaaacacc tgtgcgtcgg gtccttgcga tcatatgcaa cccattcaac
     1081 ttgcacgagc cgggctcggc cggccttgac cgcgatctgg aggttgctcg gacccggctc
     1141 gtgctggcag gcgcgaagcg caggcaaagc gggcatggcg aggcttgaaa cgagcgcctg
     1201 cgagaggacc cgggactcgt ctgttcccgg cgtcacgcgg gacgtcgggc cgagtggcag
     1261 gcgggggcgc tcatccgcag gaggcccggc gacggcggac ttcggggccg cgagctcgac
     1321 gggcgcgagg tccgcggctg cgggttcggc gagggccgac ccgatcgccg ccggttcgac
     1381 ggggctccga acctgcgtgg tcgggacggc cgagccgatc ggcggcgaga ccgggccatc
     1441 gagaccgggc cgccccgcgg cgaaaaaccc gagtacggcc agcaacacga tcgaattgat
     1501 cacgctaaag gccaggagca accctctgcc gctggacgtc ggcgccgcgg actgcgcgcg
     1561 aataaccgcg gatcgagcta tctcgaggtc tctgacgaac tggtccatgc tggcgtggcg
     1621 gtccctgggg aggagttgcg caccacattg aagctccgtg acgacgggcg cgagctcggg
     1681 ggcgaactcg ccggtgtcga tctgccgaaa gctcccggtc ttatcgtcgt agaggcgtcg
     1741 gcgtgcgagc atctcataaa gtatgaccga gaccgagaat atgtcgctct ggacggacgg
     1801 cggatgaccg ttcatgagcg ccgggtcggt gtagcccgga gatccggcga cctccatggg
     1861 tccggtcctg tattcatctt cgccgacggt gcgcagcgcc gggcggcccc tgaatctcgg
     1921 caggaggaag catataccaa agtcgatcag gcgcacgagg ccgccggcct cgaggataat
     1981 gttggtcggc tttacgtcgc gatggatcac gcccacggcg tgcgtgaccg cgaggatccg
     2041 ggccagatcg atcgctatcg tgatcgcttc gacggcctcg aatttccggg ccttcaacgc
     2101 cgaggcgagc gaaggaccct cgatgatctc catcgtgaaa tatgcgacgc catcgtcact
     2161 ccggccacag tcgtagatcg ccggtattcc ggggtggcgc agcgacgcca gggccgtata
     2221 ctcgcgcttg aaccagccct cggccgagtc gagcccgacg ttctcgggca atatgcgctt
     2281 gagcgcgacc tcgcgtccga gctcgggctg ataagccaga tgcactgtgc ccattccgcc
     2341 gcggcccagc tcgcgtagta cgtggtagcg gcccacaaag agggggctcc tcggcttggg
     2401 ggtgctcatg acacgagcgt gggcctgcgt gacgatcgtc gtcaagcccg gaacgactgg
     2461 gactattcga ccccacccct ttgccgtcga gcgaggctat tgtgggcccg atgtccgcgc
     2521 tcgtctggct cactgccatc tccctcgccg caccgctggt cacgccggtg tccacgagcc
     2581 cgcctccaga cgttatcgac gaggcgctcg agttctatga ccagggggcc tttctcgagg
     2641 ccgccgccgt ggcgatcgac ggctacgtct cgccggctcg tccccccgcg gagcgcctgg
     2701 agctcgcccg gctcgcccag gagtcgttct ccagggctta cgaggtgtcg acggcgagca
     2761 cgtcccgacc cgaatatctg tgccgggcgc tcgacgtgct cgacgcgacg gcgcccctcg
     2821 cgatcacggc caaggatcgt gaaaggcacg agaagctgcg cgcgactcac caggcgaagc
     2881 tcgcggaacg gcacccggac tttgtgtgcg aggtcggcac agagttccgg ccgatcgtgg
     2941 tgac
//
LOCUS       DASWNJ010000345         4775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_41917, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000345 DASWNJ010000000
VERSION     DASWNJ010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41917"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1304
                     /locus_tag="VGB85_06010"
     CDS             <1..1304
                     /locus_tag="VGB85_06010"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY0133611.1"
                     /translation="EAEPAVAHWVARRTTAVLAEPRWGAEIRGRIDMGAPFAIYEMVE
                     GKGCSGAGWARVGALGFACLRDAEVTKRQPKAQPPVPADRVVPFTYAKPRADAAGELL
                     APVPRYRNVAALAAGDAPVDWLQAHHQYAFIESRRVPGVGLVLLDRNRRAVRADAMEL
                     ERPSHFAGRELDARPVPDGVVAAWSAEAPTPVRKAPGLEAAVVGQIPYHREIQVDPAP
                     VEVDGLDWYALRGEPAGFVSEEDIRRWIPGAPLPGMRADETWIDVELSQQTLTLMRGQ
                     EPVFVTLISSGAGDNPTPRGIYRIWHKQALGDMRSLPGAVDSYTVEDVPWVQYFHRRF
                     ALHTAFWHNKFGRKRSHGCINLAPRDAARVFAATTPTMPPGWTFVYEHEEEAGTVVRI
                     RKGTAPVPDRRRAIGDAPAAGEETADDEPARESADDDMAAP"
     gene            1321..2172
                     /locus_tag="VGB85_06015"
     CDS             1321..2172
                     /locus_tag="VGB85_06015"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEY0133612.1"
                     /translation="MATVFFAEIDRAADAVARADALLIAAGAGMGVDSGMPDFRGGAG
                     FWQAYPAAQKLGLAFEDLANPRWFRDDPTLAWGFYGHRLNLYRETRPHQGFEVLRAWS
                     ERAPHGAFVYTSNVDGQFQSAGFDGARVVECHGSIHHMQCVRECGQALWSVDAGVEVD
                     AETLRAAPPLPSCPHCGGLARPNILMFGDADWDDGRVTEQYGRYSEWQQQLRAKKARV
                     VVIECGAGKAIPTVRLQAQRVARELGGLLVRINVREPEVPAGQIGLAMGAKAALTAID
                     ERVAALR"
     gene            complement(2176..2856)
                     /locus_tag="VGB85_06020"
     CDS             complement(2176..2856)
                     /locus_tag="VGB85_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133613.1"
                     /translation="MTPTFAIIAEGPSDFVVLRHILAGYFADSNIVATQLQPAVDDTS
                     GLRSPGGWYEVFRYIGSEKFVGAFERAEFVFVHIDTDVCEEPHFSVSRRNSDGTPRSD
                     DEMLALTTVRLIREISPTVYEQLKSRIIFAIAIESIECWLLPLYYTDRHREKQVNCIG
                     PLNAALSKQEDFSIDVNNKQTKYYFKIVKRLKRKDVVACAPHNPGYSAFVADLAAIAS
                     SHEGPASD"
     gene            complement(2877..3446)
                     /locus_tag="VGB85_06025"
     CDS             complement(2877..3446)
                     /locus_tag="VGB85_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133614.1"
                     /translation="MHGEGLFKLLEVLDSEGKLAAVKEQLALLGWFDDISIAAGVNPS
                     SRTLQLRDIFLDGATPFDQRSANEGFLFVLLYACLLISPHTPRFFAVDNVDTALNPKL
                     CAGTVRMFADLTKKYDKRVVLTAHNPGALDGLNLHDDDIRLFVVYRNTEGKTQARRIM
                     PVDVPTGTEPVRLSEAFLRGYLGALPTNF"
     gene            3668..4240
                     /locus_tag="VGB85_06030"
     CDS             3668..4240
                     /locus_tag="VGB85_06030"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY0133615.1"
                     /translation="MARAKNGEAISPAGRTAAAKKKLRRFTDAAKKRGDLDAWRRGRA
                     VLGYIDGRRVIEMATELDVTRGAINRWLQWYESTGVEGLVTGKAPGPAPKLTDSQRDL
                     LSALIEIGPQGAGYTSGVWTGPMIADLIEKHFGVRYHNHHIPRLLNGLGFSVQRPRKR
                     LARADVEAQAVWLRDKLPAIKKKRTPVAAS"
     gene            4240..4749
                     /locus_tag="VGB85_06035"
     CDS             4240..4749
                     /locus_tag="VGB85_06035"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEY0133616.1"
                     /translation="MFGDEASFWLDGTLHRTWARIGEQPRVATFGMRKTAHVFGAVSL
                     EERPRFIHQFAERFSGVTFLEFLQHLVGCFRRKIFLVIDNGPCHNLDAVGKAWLAANT
                     HRIELFRLPPYSPELNPIEGVWKTTKRTTTHNRFFRTTTDRDAALTATFERFNAHPTT
                     IAGHVARFL"
BASE COUNT          767 a         1505 c         1748 g          755 t
ORIGIN      
        1 tcgaggcgga gccggcggtc gctcactggg tcgcgcggcg gaccacggcg gtgctcgccg
       61 agccgcgctg gggcgcggag atccgcgggc gcatcgacat gggcgcgccg tttgccatct
      121 atgagatggt cgagggcaag ggctgtagcg gggccgggtg ggcgcgggtc ggcgcgctcg
      181 gcttcgcgtg tctgcgcgac gccgaggtca cgaagcgcca gccgaaggcg cagccgccgg
      241 tgccggccga ccgcgtggtg ccgttcacct acgccaagcc gagggccgac gcggcgggcg
      301 agctgctcgc gccggtgccc cgctatcgca acgtcgcagc gctggccgcc ggtgacgcgc
      361 ccgtcgactg gttgcaggcg catcatcagt acgcgttcat cgagtcgagg cgggtgcccg
      421 gggtcggcct ggtgctgctc gaccgcaacc gcagggcggt tcgcgcggac gcgatggagc
      481 tcgagcgccc gagtcatttc gccggccgcg agctcgacgc gcgcccggtg cccgacggtg
      541 tggtcgctgc ctggtcggcg gaggccccga cgccggtacg caaggcgcct ggcctcgaag
      601 ccgcggtggt ggggcagatc ccatatcacc gcgagatcca ggtcgacccc gcgccggtcg
      661 aggtcgacgg cctcgactgg tacgcgctgc gcggcgagcc ggccggcttc gtctccgagg
      721 aggacatccg ccgatggatc cccggcgcgc cgctgccggg catgcgcgcc gacgagacgt
      781 ggatcgacgt cgagctgtcg cagcagacgc tgacgctgat gcgcgggcag gagccggtgt
      841 tcgtgacgct gatctcgagc ggcgccggcg acaacccgac cccgcgcggc atctatcgca
      901 tctggcacaa gcaggcgctc ggcgacatgc gcagcctgcc gggcgcggtc gacagctaca
      961 cggtcgagga cgtgccgtgg gtgcagtact tccaccgccg cttcgcgctg cacacggcgt
     1021 tttggcacaa caagttcggc cgcaagcgca gccacggctg catcaacctc gcgccgcgcg
     1081 acgcggcgcg ggtgttcgcc gcgacgacgc cgacgatgcc gcccggctgg accttcgtgt
     1141 acgaacacga ggaagaggcc ggcacggtgg tgcggatccg caagggcacc gcgccggtgc
     1201 cggatcgcag gcgcgcgatc ggggacgcgc ccgccgcggg cgaggagacg gccgacgacg
     1261 agccggcgcg cgagtcggcg gacgatgaca tggcggcgcc ctgaggcgta cgctggcgtg
     1321 gtggcgactg tattttttgc tgagatcgat cgcgccgcgg acgcggtcgc ccgcgccgat
     1381 gccctgctga tcgcggcggg cgccggcatg ggcgtcgact ccgggatgcc cgactttcgc
     1441 ggcggcgccg gcttctggca ggcctatccg gcggcacaaa agctcgggct ggccttcgag
     1501 gatctcgcca acccgcggtg gttccgcgac gacccgacgc tggcctgggg cttttatggg
     1561 caccgcctca acctgtaccg cgagacgcgg ccgcatcagg gcttcgaggt cctgcgcgcg
     1621 tggagcgagc gggcgccgca tggcgcgttc gtgtacacgt ccaacgtcga cgggcaattt
     1681 caaagcgccg gcttcgacgg ggcgcgggtg gtcgagtgcc acggctccat tcatcacatg
     1741 cagtgcgtcc gcgagtgcgg gcaggcgctg tggtcggtcg acgcgggggt cgaggtcgac
     1801 gccgagacgc tacgcgccgc gccgccgctg ccgagctgcc cgcattgcgg cggtctggcg
     1861 cggcccaaca tcctgatgtt cggcgacgcg gactgggacg acggccgcgt gaccgagcaa
     1921 tacgggcgct acagcgagtg gcaacagcag ctgcgagcga agaaggcccg ggtggtggtc
     1981 atcgagtgcg gcgcgggcaa ggcgatcccg acggtgcgct tacaggccca gcgcgtcgcg
     2041 cgtgagctcg gcggtctgct cgtgcggatc aacgtgcgcg agccggaggt gccggcgggg
     2101 cagatcggcc tggcgatggg ggcgaaggca gcgctgacgg cgatcgacga gcgggtggcg
     2161 gcgctgcgct gagcctcagt cgctggccgg gccctcatgg gagctcgcaa tggccgcgag
     2221 gtcggccacg aatgcgctgt agcccggatt gtggggcgcg caggcgacga cgtccttgcg
     2281 tttgaggcgc ttgacgatct tgaagtaata cttggtctgc ttgttgttga cgtcgatcga
     2341 aaagtcttcc tgtttgctga gcgccgcgtt gagggggccg atgcagttga cctgcttctc
     2401 gcggtgccgg tcggtgtagt acagcggcag cagccagcat tcgatgctct caatcgcgat
     2461 ggcgaagatg atccgcgact tgagttgctc gtacacggtc gggctgatct cgcggatcag
     2521 gcgcacggtg gtgagggcga gcatctcatc gtccgagcgg ggagtgccgt cgctgttgcg
     2581 acgggagacg ctgaaatgcg gctcctcgca cacgtcggtg tcgatgtgga cgaacacgaa
     2641 ttcggcgcgc tcgaacgcgc cgacgaactt ctccgagccg atgtagcgaa acacctcgta
     2701 ccagccgccg gggctgcgca ggccgctcgt gtcgtcgacg gccggctgca gctgggtagc
     2761 gacgatgttg ctgtcggcga agtagccggc gaggatgtgg cgcaggacca cgaagtcgct
     2821 cgggccctcg gcgatgatgg cgaaggtcgg tgtcatcttg actccggacg ggaagattag
     2881 aagttggtgg gcaaggcgcc gagatacccg cgcaggaacg cctcggagag ccgtacgggc
     2941 tcggtcccgg tcggaacgtc gacgggcatg atgcgccggg cctgggtctt gccctcggtg
     3001 ttgcgataga ccacaaatag gcggatatcg tcgtcgtgaa ggttgaggcc gtcgagggcg
     3061 cccgggttgt gggcggtcag cacgacccgc ttgtcgtact tttttgtgag gtccgcgaac
     3121 atccgcacgg tcccggcgca cagcttgggg ttgagggcgg tgtcgacgtt gtcgacggcg
     3181 aagaagcgcg gggtgtgcgg gctgatcagc aggcaggcgt agaggaggac gaacaaaaag
     3241 ccctcgttgg cgctgcgctg gtcgaagggt gtcgcaccgt cgaggaagat gtcgcgcagc
     3301 tgcagcgtgc gcgaggacgg gttgacgccc gcggcgatcg agatatcgtc gaaccacccg
     3361 agcaaggcga gctgctcctt gaccgcggcg agcttgccct ccgagtcgag gacctcgagt
     3421 aatttgaaga ggccctcgcc gtgcacgccg atcggctcga cgtcgctgtc gcgggcgagg
     3481 tcacgtaaga cgtcttcgcg gggggcgtaa gcgaggtatt gcgtgaggcc gagtttaaac
     3541 aggatctcgt ggatccagat ccaagcgtga gaaggatccg gagtgatgag cccggttgat
     3601 ccaggtgctt gggttgcgag gggccacgac cgaggccatt ttggcgtgag caccaaggag
     3661 gtagctcatg gcacgcgcca agaatggcga ggcgatcagc ccggcggggc gtacggcggc
     3721 ggcgaaaaag aagctgcgtc ggtttacgga tgccgcgaag aagcgggggg acttggacgc
     3781 gtggcgaagg ggccgcgccg tgctcgggta catcgatggg cgccgagtca tagaaatggc
     3841 gacggagctg gacgtgacgc gaggggcgat caatcgctgg ctgcagtggt acgagtcgac
     3901 gggcgtcgag ggcttggtga cgggcaaagc cccggggccg gcgccgaagc tgacggactc
     3961 gcagcgggac ctcttgagcg cgctcatcga gatcggtcca caaggagccg gctacaccag
     4021 cggcgtctgg acgggtccaa tgatcgcgga cctcatcgag aagcacttcg gcgttcggta
     4081 ccacaaccat cacatcccgc ggctgctcaa cgggctcggg ttctccgtcc agcgcccacg
     4141 caagcgcctt gcccgcgccg atgtcgaggc ccaagcggtt tggctccgcg acaagttgcc
     4201 ggcgattaaa aaaaagcgaa cgcctgtcgc ggcatcgtga tgttcggcga cgaggcgagt
     4261 ttctggctcg acggtacctt gcaccgcacc tgggcgagga tcggcgagca gccacgcgtg
     4321 gcgaccttcg gcatgcgcaa gacagctcat gtcttcggtg cagtctccct tgaggagagg
     4381 ccgcgcttca ttcaccagtt cgcggagcgg ttcagcggag tgacctttct ggagttcctc
     4441 cagcacctcg tcggctgctt ccgccgcaag atcttcctcg tcatcgacaa cgggccctgc
     4501 cacaacctcg acgcggtcgg caaggcgtgg ctcgccgcca acactcaccg catcgagctc
     4561 ttccgcctgc cgccctactc ccccgaactc aaccccatcg agggcgtctg gaagacaacc
     4621 aagcggacca ctacccacaa ccgcttcttc cggaccacca ccgaccgcga cgccgcgctc
     4681 accgcgacct tcgaacgatt caacgcccac ccgaccacca tcgcaggcca tgtcgcacgc
     4741 ttcctttgat ctccggtcta tgcgactctg tttag
//
LOCUS       DASWNJ010000346         6925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_183213, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000346 DASWNJ010000000
VERSION     DASWNJ010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6925
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183213"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1477
                     /locus_tag="VGB85_06040"
     CDS             <1..1477
                     /locus_tag="VGB85_06040"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEY0133617.1"
                     /translation="EDTMPITLPRRTLLRGLGGVTLALPFLEIMQPQRATAAPGDTPK
                     RYLYSFAGSSLGMDATPNMVAPKQEGLAYDITRGLQPIADLGVQSHVSVVSGLKIPWG
                     TLDTAPAGGRIVRFHATSLCPLVCGMRSDPNTEAATGQTSDWTVSQTISAPGQSIMNF
                     RVQPRFYRGGNESTSARGRISARVVDGVLQHEEPNFSPRSAWNSLFANFIPTDPAEKL
                     KAEFLLRRRKSIVDLVRGDTERLVAKLGGADKQRMQRHLDELRGLELRLQAIEPPGDG
                     ACMQPADPGDDPPEGGAIDNGDTNGYANNGAWSNEELRAEVFTNLVYMAFTCDLSRVA
                     SLMYTWSQCFMNLNPVFDYPSDLHELGHFSVGGGEAGTGAVADGVSWHVKHFAKLTKL
                     LADTPDIDGTTVLDNTAMVLAFEGGHGYDPEGDKPDSAHSTENMVVVIAGHAGGLNKS
                     GGRHVLGTDRHPVEVVNTAMHAVGVAGDLGEVTGEFSELFT"
     gene            complement(1487..2251)
                     /locus_tag="VGB85_06045"
     CDS             complement(1487..2251)
                     /locus_tag="VGB85_06045"
                     /inference="COORDINATES: protein motif:HMM:NF021527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe dioxygenase family protein"
                     /protein_id="HEY0133618.1"
                     /translation="MLIDLDHALTAADARARLVSQGFAVLTPPALAQLAGHPLAELTA
                     LAPFWDDLPHDRYLRDLGRYRSRRHGCAVVHVEGDDARLAEVPHRPHWQSTAYNALHG
                     GLERWFEPIDRRMAQAAVWRDLLTALGRCVAGVRAPVDGRWYVEAHPFRIDTSEGVGR
                     PTPEGAHRDGVDFVAVILVGRHAITGGETRVFEAAGPHGVRFSLATPWTALLLDDTRV
                     IHESTPIQPLADDARGHRDTLVLTYRAGGFQAPPES"
     gene            complement(2394..3233)
                     /locus_tag="VGB85_06050"
     CDS             complement(2394..3233)
                     /locus_tag="VGB85_06050"
                     /inference="COORDINATES: protein motif:HMM:NF023805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M57 family metalloprotease"
                     /protein_id="HEY0133619.1"
                     /translation="MAAAGVAGFEEGVILMLLRNACLLSVVVLFGCDGVDVEPTGLDA
                     NAEIVANLKVAGYPESEIAVVGDQVIAGGDAVVSLEASREMIGLADGHEHEDGTQFRQ
                     YRTSNLVDPSVAVICVDGSAWTGTLSQALDASIANYTNLGLSFDMKRTSGSTAGCDAV
                     IKATSKGGAGGSSGFPEGGLPYDTINVGKSTTSYGLAVTTHVVTHELGHCVGFRHTDY
                     YNRTISCGTGGNEGDGGVGAIHIQGTPTTAVIDGSVMNSCFNGGSTGKWTASDVTALN
                     AVY"
     gene            3824..5050
                     /locus_tag="VGB85_06055"
     CDS             3824..5050
                     /locus_tag="VGB85_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133620.1"
                     /translation="MLGCLVLLLTACELEAPAPRALSTPPADLLARPLVRVARADGGP
                     ALVLAAPAPAALDRFARALADDPQLGLDPLAYQAFVTPELHWQDGDWRPADARSQVLR
                     GEDPVLGDSFLVDLITVDAPGGPRHVLHPLAAIATPTPSPVDPALLVIERELALFLVD
                     VAAGQVQHLGDPATRRAALAAAAEVPLVDEGPAVVWATAPQWSPDGAQIAFLSTRGGE
                     PTTPELWVHNLDHGDERRVAAPGVQVRLLGWHGDDTLLVEDHDDPARPRLAAIDLDDG
                     ALRPLADGAVIGRSPDGDTLALAEGPPGRLRVDLLDLSSGTSTTSVALRPGELLRSWQ
                     ADFSDDGAQLVLDTTDADGRQSLLVVDHEGAATRVPLPGRGQLAAAPTWLGERLLVPL
                     EDLATGTTTTHLVTVP"
     gene            5060..6001
                     /locus_tag="VGB85_06060"
     CDS             5060..6001
                     /locus_tag="VGB85_06060"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY0133621.1"
                     /translation="MPMTRTTTRFALVLGLGLALTAGAAHAASYAVVFGGLGTGAGTT
                     NLGNPHTTVYTVTSKFNQPRNVNGTNPHRGTDLGSPYGTNVLSPWNGWVALAAPGSYE
                     LTLRLDINNDGLKNDSVYMKFDHLSAVLIANGTAVTKGQTVGRVGNEGGVYASHLHFG
                     LMKDLDNNATADVWIRNEPYYRTVTGWDFGKMLDFIALSTFSSNKAQVTCYSKDEVGK
                     QDVAAADVVFYHRKVGSTFWNANAATKVGDVFSFDLKSTYPAATQVQWMVRAHRTSIK
                     GKISYTWAYMPPKFDQPDHSPNTAPSNVFDYYVNTVQ"
     gene            6136..6912
                     /locus_tag="VGB85_06065"
     CDS             6136..6912
                     /locus_tag="VGB85_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133622.1"
                     /translation="MTTMRLTHSSLPWLCCLALAACGDSGGRDNGVTATTMGPTAVTL
                     TSPSTGEDTPTTGSSEAGTTQEPTGTDTGDACTAETCPDGECIAGACCPLELACTSQC
                     CGEGQVCSFQQCVVPGVECIDASECPADNYCEYSLGDPGMMGGDLCQGGAQPATGKCL
                     PTPPQCDPGVVPEGDDIDCLPQCEVIPEKSFEPVLKYEWTGGDVMMPPIVIQLDDDNC
                     DLVVDERDIPEIVFSSFEGGDYNNNGTLRAVSVKDGAFVE"
BASE COUNT         1095 a         2589 c         2251 g          990 t
ORIGIN      
        1 ggaggacacc atgccgatca cgctcccgag acgaaccctt ctgcgtggac tgggcggagt
       61 caccctcgcc ctgcccttcc tcgagatcat gcaaccgcag cgggccacgg ccgcgcccgg
      121 ggacaccccg aagcgatatc tatacagttt cgccggcagc tcgctcggca tggacgcgac
      181 ccccaacatg gtcgcgccca agcaggaggg cctcgcctac gacatcacgc gcggcctcca
      241 gccgatcgcc gacctcggcg tgcagtcgca cgtatcggtg gtctccggcc tgaagatccc
      301 gtggggcacg ctcgacaccg cgcccgctgg cggtcgcatc gtccgcttcc acgcgacctc
      361 gctgtgcccg ctcgtctgcg gcatgcgctc ggaccccaac accgaggccg cgaccggcca
      421 gacctccgac tggaccgtgt cgcagacgat cagcgcgccc ggccagtcga tcatgaactt
      481 tcgcgtgcag ccccgcttct accgcggcgg caacgagtcc acctcggcgc gcggacggat
      541 ctccgcgcgc gtcgtcgacg gcgtgctgca acacgaggag ccgaacttca gcccgcgctc
      601 ggcctggaac tcgctgttcg ccaacttcat cccgaccgac cccgccgaga agctcaaggc
      661 cgagttcctg ctgcgccgcc gcaagagcat cgtcgacctc gtccgcggcg acaccgagcg
      721 cctggtcgcc aagctcggcg gcgccgacaa gcagcgcatg cagcgtcacc tcgacgagct
      781 gcgcggcctc gagctgcgcc tgcaggcgat cgagccgccg ggcgacggcg cgtgcatgca
      841 acccgccgac cccggcgacg acccgcccga gggcggcgcg atcgacaacg gcgacaccaa
      901 cggctacgcc aacaacggcg cgtggagcaa cgaggagctg cgcgccgagg tgttcaccaa
      961 tctcgtctac atggccttca cctgcgacct ctcgcgcgtc gcctcgctga tgtacacgtg
     1021 gtcgcagtgc ttcatgaacc tcaacccggt gttcgactac ccgagcgacc tgcacgagct
     1081 cggccacttc tcggtcggcg gcggtgaggc cggcaccggc gcggtcgcgg acggcgtctc
     1141 gtggcacgtc aagcacttcg ccaagttgac gaagctgctc gccgacactc ccgacatcga
     1201 cggcacgacg gtcctcgaca acaccgcgat ggtgctcgcc ttcgagggcg gccacggcta
     1261 cgaccccgag ggcgacaagc ccgactccgc gcattcgacc gagaacatgg tcgtcgtgat
     1321 cgcgggccac gcgggtggcc tcaacaagag cggcggccgc cacgtgctcg gcaccgaccg
     1381 ccacccggtc gaggtcgtca acacggcgat gcacgcggtc ggcgtcgcgg gcgacctcgg
     1441 cgaggtcacc ggcgagttct ccgagctgtt cacatagccc gcgggctcag gactccggcg
     1501 gggcctggaa cccgccggcg cggtacgtga gcaccagcgt gtcgcgatgt ccgcgggcgt
     1561 cgtcggcgag cggctggatc ggcgtgctct cgtggatgac gcgggtgtcg tcgagcagca
     1621 gggcggtcca cggcgtcgcc agcgagaagc gcacgccgtg cggccccgcc gcctcgaaca
     1681 cgcgggtctc accgccggtg atcgcgtggc gaccgacgag gatcacggcg acgaagtcga
     1741 ccccgtcgcg gtgcgccccc tcgggcgtcg ggcgaccgac cccctccgac gtgtcgatgc
     1801 gaaacgggtg cgcctcgacg taccagcgcc cgtcgaccgg cgcgcgcacg ccggcgacgc
     1861 agcgcccgag cgccgtcagc aagtcgcgcc ataccgcggc ctgtgccatg cggcggtcga
     1921 tcggctcgaa ccaccgctcg aggccgccgt gcagcgcgtt gtaggcggtc gactgccagt
     1981 gcgggcggtg cgggacctcg gcgaggcgcg cgtcgtcccc ctcgacgtgc acgacggcgc
     2041 agccgtggcg ccgcgatcga taacgcccga ggtcgcgcag gtaacggtcg tgcggcaggt
     2101 cgtcccaaaa cggcgcgagc gcggtgagct cggccagcgg atgaccggcg agctgcgcca
     2161 gcgccggcgg cgtcagcacg gcgaagccct gagagacgag gcgggcgcgg gcgtcggctg
     2221 cggtcagcgc gtggtcgagg tcgatgagca cgggcccgca tcctgcccgc tgccggccgg
     2281 atctccaaac cccttcaagg gcgagaacgc gagcggcccg gggctctgac ttcagatccc
     2341 cgggccaacg ctgttcattt ttacttttgc gcctccccca tcggggagcg cgatcaatag
     2401 accgcgttga gggcggtcac gtcgctcgcc gtccacttgc ccgtgctgcc accattaaaa
     2461 cacgagttca tgaccgagcc gtcgatgacg gcggtggtcg gggtgccctg gatgtggatc
     2521 gcaccgacgc cgccgtcgcc ctcgttgccg ccggtcccgc agctgatggt acgattatag
     2581 taatcggtgt ggcgaaagcc gacgcagtga ccgagctcgt gggtgaccac gtgggtggtc
     2641 accgcgaggc cgtagctggt cgtcgacttg ccgacgttga tcgtatcgta gggcagcccg
     2701 ccctccggga agcccgaaga tccgccggct ccgcccttgc tcgtcgcctt gatcaccgcg
     2761 tcgcagcccg cggtcgagcc gctggtgcgc ttcatgtcga aggacaggcc gaggttggtg
     2821 tagttggcga tcgacgcgtc gagcgcctgg ctcagcgtgc ccgtccaggc cgaaccatcg
     2881 acgcagatca cggcgacgct cgggtcgacg aggttgctcg tgcggtactg ccggaactgc
     2941 gtgccatcct cgtgctcgtg cccgtccgcg aggccgatca tctcgcgcga ggcctcgagc
     3001 gagacgaccg cgtcgccacc ggcgatcacc tggtcgccga cgaccgcgat ctcgctctcg
     3061 ggataacccg cgaccttgag attggcgacg atctccgcgt tggcgtcgag acccgtcggc
     3121 tcgacatcca caccatcaca gccgaagaga accacaacag acagcagaca cgcgttcctc
     3181 agcagcatca ggatcacccc ttcttcaaag ccggccactc ccgcagcggc cacgacagca
     3241 ccctacgagc tttgctgcct ttagttcaag atgtatcttc atcgaaagtc cgtgaatcgg
     3301 gcgaggtggg acgcgggcac acatgacaag gattttacgc cccggtgccc ctacgcgcac
     3361 gatctgagcg taaaacagcg acaccgatgg cactcacccc ctgggccgca cacgtcatgg
     3421 ccgtggacga cagcgagcat tgccggcgcg tcgatcaatc ctcgctgtgg cgcccgcgcc
     3481 gccagtctgc cgacaggtct gctctcggcg gcaatgcgcc cctcgcattt cgctccggtc
     3541 caccgcgata catgtgcacg ctggatatgt gcaccgagga atctacgcga ccgcccctgc
     3601 gcacctcgtc cctgcaggcc gtccgcgcgc gcgcgggcca gggcggctgt aagcacattc
     3661 cacacgccac ggcgctcgac gcggggcggg gtcaaacgac cccgcgttta cgctgaacag
     3721 ccccgccacg cggcgatgtt cgatccactt agatcccgtt actttgaaaa tgccgcggga
     3781 cttgccccgc gcggggagct acgccgtgcg atccgaagta tcgatgttgg gctgtctcgt
     3841 cctgctgttg accgcttgcg agctcgaggc gccggcgcca cgggccctgt ccacgccgcc
     3901 tgccgacctg ctcgcacgac cgctggtccg cgtcgcgcgg gccgatggtg ggccggcgct
     3961 ggtgctcgcg gcgcccgccc ctgccgcgct cgaccggttc gcgcgcgccc ttgccgacga
     4021 cccgcagctc ggcctcgacc cgctcgccta ccaggccttc gtcacgcccg agctgcactg
     4081 gcaggacggc gactggcgcc cggccgatgc ccgatcacag gtcctccgcg gtgaagatcc
     4141 tgtcctcggg gacagcttcc tcgtcgacct gatcaccgtc gacgcgccgg gcgggccgcg
     4201 ccacgtcctt cacccgctcg ccgcgatcgc cacgccgacg ccgtcgccgg tcgatccggc
     4261 gctgctggtg atagaacgcg agctggcatt gttcctcgtc gacgtcgccg ccggtcaggt
     4321 ccagcacctc ggagatcccg cgacgcgtcg cgccgcgctc gcggccgcgg ccgaggtccc
     4381 gctcgtcgac gagggcccgg cggtggtgtg ggcgaccgcg ccgcagtggt cgcccgatgg
     4441 cgcgcagatc gcattcctga gcacccgcgg cggcgagccg acgacgcccg agctgtgggt
     4501 gcacaacctc gaccacggcg acgagcggcg ggtcgccgcc ccgggcgtgc aggtccgcct
     4561 gctcggctgg cacggcgacg acacgctgct cgtcgaggat cacgacgacc ccgcgcgccc
     4621 gcgcctggcc gcgatcgacc tcgacgacgg cgccctgcgc ccgctcgccg acggcgcagt
     4681 gatcgggcgc agccccgacg gtgacacgct cgcgctcgcc gagggcccgc ccgggcgcct
     4741 gcgcgtcgac ctcctcgacc tgtcctcggg gacatccacg accagcgtcg cgctgcgccc
     4801 cggcgagctg ctgcgctcgt ggcaggccga cttctcggac gacggcgcgc agctcgtgct
     4861 cgacacgacc gacgccgacg gccgccagtc gctgctcgtc gtcgaccacg agggcgccgc
     4921 cacccgcgtg ccgctgccgg gccgtggcca gctcgccgcg gcgccgacct ggctcggcga
     4981 gcgcctgctc gtcccgctcg aggacctcgc caccggcacc acgaccaccc acctcgtcac
     5041 tgttccctga ggaccgacca tgcccatgac gcgcacgacc acccgcttcg ccctcgtcct
     5101 cggcctcggc ctcgccctca ccgccggggc cgcccacgct gccagctatg ccgtcgtctt
     5161 cggcggcctc ggcaccggcg ccggcacgac caacctcggc aacccgcaca cgacggtgta
     5221 cacggtgacg agcaagttca accagccgcg caacgtcaac ggcaccaacc cgcaccgcgg
     5281 caccgacctc ggatcgccct acggaaccaa cgtgctgtcg ccgtggaacg gctgggtcgc
     5341 cctcgccgcg cccggcagtt acgagctgac gctgcggctc gacatcaaca acgacgggct
     5401 caagaacgac agcgtgtaca tgaagttcga ccatctctcg gcggtgctca tcgccaacgg
     5461 cacggccgtg accaagggtc agacggtcgg ccgcgtcggc aacgagggcg gcgtttatgc
     5521 ctcgcacctg cacttcgggc tgatgaagga cctcgacaac aacgcgaccg ccgacgtatg
     5581 gatccgcaac gagccgtact accgcaccgt cactggctgg gacttcggca agatgctcga
     5641 cttcatcgcg ctgtcgacgt ttagcagcaa caaggcgcag gtcacctgct actccaagga
     5701 cgaggtcggc aagcaggacg tcgccgccgc cgacgtcgtc ttttatcacc gcaaggtcgg
     5761 ctcgaccttc tggaacgcca acgccgcgac caaggtcggc gacgtgttct cgttcgacct
     5821 caagtccacg taccccgcgg ccacccaggt gcagtggatg gtccgcgcgc accgcacgtc
     5881 gatcaagggc aagatcagct acacctgggc gtacatgccg cccaagttcg accagcccga
     5941 tcacagcccc aacaccgcgc cgagcaacgt gttcgactac tacgtcaaca ccgtgcaata
     6001 gccgccgcgg cgctgcatgt cgcgcatcgc cggcgcgaaa gcgcctgacc gcgcccccat
     6061 gcgggcgcgg tcgcgcgacc agcagtcaca aggcgtctcg cgcgcgctgg acggggcggc
     6121 ggcctcgatg ttcccatgac gacgatgcgc ctcacccact cctcgctgcc ctggttatgt
     6181 tgcctcgccc tcgccgcctg tggtgacagc ggcggtcgtg acaacggcgt caccgcgacc
     6241 accatgggtc cgacggccgt cacgctcacc agcccgagca ccggcgagga cacgccgacc
     6301 accggcagca gcgaggccgg caccacgcag gagccgaccg gcaccgacac cggcgacgcc
     6361 tgcaccgccg agacctgccc cgacggcgaa tgcatcgcgg gcgcctgctg cccgctggag
     6421 ctcgcgtgca cgagccagtg ctgcggcgag gggcaggtgt gttcattcca gcagtgcgtc
     6481 gtgcccggcg tcgagtgcat tgacgccagc gaatgcccgg ccgacaacta ctgtgaatac
     6541 tcgctcggcg acccgggcat gatgggcggc gacctgtgcc agggcggcgc ccagccggcg
     6601 accggcaagt gcctgccgac cccgccgcag tgcgaccccg gcgtcgtgcc ggagggcgac
     6661 gacatcgact gcctgccgca atgcgaggtc atcccggaga agagcttcga gcccgtgctc
     6721 aagtacgagt ggaccggcgg cgacgtcatg atgccgccga tcgtcattca gctcgacgac
     6781 gacaactgtg atctcgtcgt cgacgagcgc gacatcccgg agatcgtgtt tagctcgttc
     6841 gagggcggcg actacaacaa caacggcacc ctgcgcgccg tctcggtcaa ggacggcgcc
     6901 ttcgtcgagt agtgggccgt caagc
//
LOCUS       DASWNJ010000347         2819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_77774, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000347 DASWNJ010000000
VERSION     DASWNJ010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77774"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1519)
                     /locus_tag="VGB85_06070"
     CDS             complement(<1..1519)
                     /locus_tag="VGB85_06070"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF012611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase, GntK/IdnK-type"
                     /protein_id="HEY0133623.1"
                     /translation="MIVVVMGVSGSGKTTVGELLAQRWRCRFSDADGFHPPANLEKMR
                     SGVPLGDADRLPWLRTIRDAMAASLAAGTDHVIACSALKRAHRQILAPDGSPEREVVF
                     VHLRGSPDLVRARLGARTGHFFDPTLLQSQLEALEAPEDAITVDILQTPAQIVAELTR
                     VLRPEHLEDPMPLSTNPSIGLVGLGVMGRNLAANLADHGVTVVGLDLGLAQRTAFASA
                     VPTGIPCATLAELLDQLPSPRRILLMVPAGAPVDALLTALLPALARGDVVLDGGNSHY
                     RDTQARARRAAEAGIDYIGLGVSGGEQGARHGPSLMAGGTVEAIEEVAPLLRAIAARA
                     PDGAPCFARVGPDGAGHFVKMIHNGIEYADMQLIAEGYHLLRTLGRSSYAQLADTFAA
                     WNAGELGSYLMEITTAILRTRDPETGAPILEVIRDAAGQKGTGHWASTTAMELGMPAP
                     TIAEAVYARSLSALKDERLHAAAALPIAAPSPPPADLAAIVGDALLAARIAVYAQG"
     gene            1735..>2819
                     /locus_tag="VGB85_06075"
     CDS             1735..>2819
                     /locus_tag="VGB85_06075"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA repair domain-containing protein"
                     /protein_id="HEY0133624.1"
                     /translation="MQDVIGRIRFDAALDVSRFTVGYEERFAGVREAPLAGVLGEGEV
                     PWHRIWFIKAGSLVVWDRRERIDLILGSGASPAADLAAVQRACAPEPAPVVTATRSKA
                     GAGARFVPQPCFRFDRMLGEWVAHDTVDAAVVAPESLQVVTYNVLFDLYEAEHIYSEQ
                     RRVACIELLRAREADIIALQEVTPQLWAQLLATPWVQDAYYVSAGPDAGGLVPYGQAL
                     LSRWPLALEVHAFSAQKRLLVGRLALAGRSLAVAAVHLTSNHKADALDKRREQLDVLT
                     QWLAREPGDALVLGDFNFGDGDENPRLAAAGLVDAWQAVHPHHPGFTFDPVANPLAAL
                     NSRTGNAARYDRVLLYSPGRALAPIDIT"
BASE COUNT          385 a          941 c         1104 g          389 t
ORIGIN      
        1 agccctgggc gtagacggcg atgcgggcgg cgagcagcgc gtcgccgacg atcgcggcga
       61 ggtcggcggg cggcggcgat ggggcggcga tcggcagggc ggcggcggcg tgcaggcgtt
      121 cgtccttgag cgccgagagg ctacgagcgt acacggcctc ggcgatcgtc ggggccggca
      181 tgccgagctc catcgccgtc gtcgacgccc agtggccggt gcccttctgg ccggcggcgt
      241 cgcggatgac ctcgaggatc ggggcgccgg tctccgggtc gcgcgtgcgc aggatcgcgg
      301 tcgtgatctc catcaaatag gagccgagct cgcccgcgtt ccaggcggcg aaggtgtcgg
      361 ccaattgtgc gtaggaagat cgaccgaggg tgcgcagcaa atgatagccc tcggcgatca
      421 gctgcatgtc ggcatattcg atcccgttgt ggatcatctt gacgaagtgg ccggcgccgt
      481 cggggccgac gcgggcgaag cagggcgcgc cgtcgggggc acgcgccgcg atcgcgcgca
      541 gtagaggagc gacctcttcg atcgcctcga cggtgccgcc ggccatcagc gaggggccgt
      601 ggcgggcgcc ctgctcgccg cccgagacgc cgaggccgat gtaatcgatc cccgcctcgg
      661 cggcccggcg ggcgcgcgcc tgggtgtcac gataatgcga gttgccgccg tcgagcacga
      721 cgtcgccgcg ggcgagcgcg ggcagcaggg cggtcagcag ggcgtcgacg ggcgcgccgg
      781 cgggcaccat caggaggatc cggcgggggg atggcagctg gtcgagcagc tcggccaggg
      841 tcgcgcaggg gatgccggtc ggcacggcgc tggcgaaggc ggtgcgctgc gcgaggccga
      901 ggtcgaggcc gacgacggtg acgccgtggt cggcgaggtt ggcggcgagg ttgcggccca
      961 tgacgccgag gccgacgagg ccgatcgaag ggttggtgga tagtggcatc gggtcctcga
     1021 ggtgttcggg tcgcagcacg cgggtcagct cggcgacgat ctgcgcgggg gtctggagga
     1081 tgtcgacggt gatcgcgtcc tccggcgcct cgagggcctc gagctggctc tgcagcaggg
     1141 tcgggtcgaa gaagtgtccg gtgcgcgcgc cgagccgcgc gcggacgagg tccggcgagc
     1201 cacgaagatg cacgaacacg acctcgcgct cggggctgcc atcgggggcg aggatctggc
     1261 ggtgggcgcg tttgagggcc gagcaggcga tcacgtggtc ggtgccggcg gcgagcgagg
     1321 cggccatcgc gtcgcggatc gttcgcagcc atggcaggcg gtcggcgtcg ccgagcggga
     1381 cgccgctgcg catcttttct aaattggccg gcggatgaaa gccgtcggcg tcgctgaagc
     1441 gacagcgcca gcgctgcgcg agcagctcgc cgacggtggt cttgcccgag ccggacacgc
     1501 ccatcacgac gacgatcatg cgcgcgcgct ccggcgggga gggatcatgc ccgcggacgc
     1561 tagcccgatt ttggccgcgg gcccagccgc gggcggcgat cttttttgcg aggggcttcc
     1621 aaacgcgtga atagccgcgc aaactcgccg tcggaggcgc cccctggacc ggccatggca
     1681 aaaccacgcg acaaacgcgg gccgacccct cggcccgacc gcggccttcg gcccgtgcag
     1741 gacgtcatcg gcaggatccg cttcgacgcg gccctcgatg tctcccgctt cacggtaggc
     1801 tacgaggagc gcttcgcggg tgtgcgcgag gcgccgctcg ccggggtgct cggcgagggc
     1861 gaggtcccgt ggcaccgcat ctggttcatc aaagccgggt cgctggtcgt gtgggaccgg
     1921 cgcgagcgca tcgacctgat cctcgggtcc ggcgcgagcc cggcggcgga cctcgccgcg
     1981 gtccagcgcg cctgtgcgcc cgagccggcg ccggtcgtga cggcgacccg cagcaaggcg
     2041 ggcgccggcg cgaggttcgt cccgcagccg tgctttcgct tcgaccggat gctcggcgaa
     2101 tgggtcgcgc acgacaccgt cgatgcggca gtcgtcgcgc ccgagtcgct gcaggtcgtc
     2161 acatacaacg tgctgttcga cctctacgag gccgagcaca tctacagcga gcagcgccgg
     2221 gtcgcgtgta tcgagctgct gcgcgcgcgc gaggccgaca tcatcgcctt gcaggaggtg
     2281 acgccgcagc tgtgggcgca gctgctggcg acgccgtggg tgcaggacgc gtattacgtg
     2341 tcggcgggcc cggacgcggg cgggctggtg ccatacggcc aggccctgct gtcgcgctgg
     2401 ccgctggcgc tcgaggtgca tgcattctcg gcgcaaaagc ggctgctcgt cggacgcctc
     2461 gcgctcgcgg gccgctcgct ggcggtcgcg gcggtccacc tgacgagtaa tcacaaggcg
     2521 gacgcgctcg acaagcggcg cgagcagctc gatgtcctga cgcaatggct ggcgcgcgag
     2581 cccggcgacg cgctggtgct cggcgacttc aacttcggcg acggcgacga gaacccgcgc
     2641 ctggcggcgg ccgggctcgt cgacgcgtgg caggccgtgc acccgcacca cccgggcttc
     2701 accttcgacc cggtcgccaa cccgctcgcg gcgctcaaca gccgcaccgg caacgcggcc
     2761 cgctacgacc gcgtgctgct gtactcgccg ggccgggcgc tcgcgccgat cgacatcac
//
LOCUS       DASWNJ010000348         1615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_6888, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000348 DASWNJ010000000
VERSION     DASWNJ010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6888"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VGB85_06080"
     CDS             complement(<1..616)
                     /locus_tag="VGB85_06080"
                     /inference="COORDINATES: protein motif:HMM:NF019644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY0133625.1"
                     /translation="MPGNSARRGAIKKTGKGNPTAGSGGRVKRGLEGRGPTPKAKDRE
                     GHVAYKARKRAEKPAPRRRSAAKASDADWIAGRNSVVEALRASMPVSAVYVAEGAERD
                     GRLREVFRVAADRGISLLEVPRNELDRLTDGAVHQGLAARVPAYEYAHPDELLARARA
                     MGEEPLIVALDSVTDPRNLGAVVRSAAGFGAHGVVVPERRAAGMT"
     gene            complement(678..>1615)
                     /locus_tag="VGB85_06085"
     CDS             complement(678..>1615)
                     /locus_tag="VGB85_06085"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HEY0133626.1"
                     /translation="HPGIDDLDAATDADPQTTARKRDPRDFALWKGQKPSEPSTAAWP
                     SPWGRGRPGWHIECSAMALKYLGGAFDIHGGGVDLRFPHHENEQAQSRAAGFDFASYW
                     MHNAWITTSGQKMSKSLGNSLVVSAVLERVRAIDLRFYIVAAHYRSHVEFSHQALEEA
                     AAGFARIEQFLTRTPRAVELVETLPESFVAAMDDDLGTPAAVAVIYDAVREGNRLLAS
                     GAIDELQAVASSVRSMLAVLGLDPFDPHWSTGGTSGAEARLTAAVDALVATLLEQRTE
                     ARAARDFAAADAIRDQLKAAGIDLEDTPEGPRWSL"
BASE COUNT          193 a          607 c          584 g          231 t
ORIGIN      
        1 cggtcatccc ggccgccctg cgctcgggga cgaccacacc gtgggcgccg aagccggcgg
       61 cgctgcggac gacggccccg aggttgcgcg ggtcggtcac cgagtccagg gccacgatga
      121 gcggctcctc ccccatcgcg cgcgcccggg ccaggagctc gtcggggtgc gcgtactcgt
      181 acgccggcac ccgcgccgcc agaccctggt gcaccgcccc gtccgtcagc cggtcgagct
      241 cgttgcgggg cacctccaac agcgagatgc cgcggtcggc ggccacccgg aacacctcgc
      301 gcagccgacc gtcgcgctcc gcgccctcgg cgacgtacac cgcgctcacc ggcatgctcg
      361 cccgcagcgc ctcgacgacg gaattgcgcc cggcgatcca gtcggcgtcg ctcgccttgg
      421 ccgccgacct gcgtcgcggc gccggcttct cggcgcgctt gcgggccttg taggcgacgt
      481 gtccctcgcg gtccttcgcc ttgggggtcg gcccgcggcc ctcgaggccg cgcttgaccc
      541 ggccgcccga gccggcggtg ggattgccct tgccggtctt cttgatcgcg ccgcgtcgtg
      601 cggagttgcc cggcatcagc tgtcctctgt tcgtgtgtgc ggtctcgacg agctcgaccg
      661 cccgcgggcc cgccccgtca cagggaccac cggggaccct cgggggtgtc ctcgaggtcg
      721 atgccggcgg ccttgagctg gtcgcggatc gcgtcggcgg cggcgaagtc gcgggccgcc
      781 cgggcctcgg tgcgctgctc gagcaacgtg gcgaccaagg cgtccacggc cgcggtcagc
      841 cgcgcctcgg caccggaggt gccgccggtc gaccagtgcg ggtcgaacgg gtccagcccg
      901 agcaccgcga gcatcgaccg caccgacgag gcgacggcct gcagctcgtc gatcgcgccg
      961 gaggccagca gccggttgcc ctcgcggacc gcgtcgtaga tcaccgcgac cgccgccggg
     1021 gtgccgaggt cgtcgtccat ggccgccacg aaggactcgg ggagtgtctc gacaagctcg
     1081 accgctcggg gagtccgggt caggaactgc tcgatccggg cgaacccggc cgcggcctcc
     1141 tcgagcgcct ggtgggagaa ctcgacgtgc gagcggtagt gcgccgcgac gatgtagaac
     1201 cgcaggtcga tcgcgcgcac ccgctccagc accgcggaga ccacgagcga gttgccgagc
     1261 gacttgctca tcttctgccc ggacgtggtg atccaggcgt tgtgcatcca gtacgacgcg
     1321 aagtcgaacc cggccgcgcg cgactgggcc tgctcgttct cgtggtgcgg gaaccgcagg
     1381 tcgacgcctc caccgtggat gtcgaaggcg ccgccgaggt acttcagcgc catcgccgag
     1441 cactcgatgt gccagccggg ccgtcctcgc ccccacggcg agggccacgc tgccgtcgac
     1501 ggctcgctgg gcttctggcc cttccacagc gcgaagtcgc gagggtcgcg cttcctggcc
     1561 gtcgtctgcg ggtccgcatc ggtggcggcg tccaggtcgt cgatgcccgg gtggg
//
LOCUS       DASWNJ010000349         3040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_254416, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000349 DASWNJ010000000
VERSION     DASWNJ010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254416"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VGB85_06090"
     CDS             complement(<1..436)
                     /locus_tag="VGB85_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133627.1"
                     /translation="MFNFFTTTSSRSQLLATSRSRLLATSILAASVLAACGDNTAMES
                     SSDTSTTGTPPETTTPVTTDPVTTTGELVLLCEPGAERCADSVTREICKPTGLQWEPV
                     SCGNYQTCNEEVGEEGTIAKCEGPCEVAETNPTSVGCEFLAIR"
     gene            complement(458..1801)
                     /locus_tag="VGB85_06095"
     CDS             complement(458..1801)
                     /locus_tag="VGB85_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133628.1"
                     /translation="MKRTNALGTACIGLLAGVLASLAVPACRGEDIELDCTSQQQAFT
                     DAFADDGVLLPDISESSLYPMAMVLSETGIQNLLNGLVGGNVPFASKLDIGPYGLNFI
                     PQGTPTIQIVKVPNCSSCVLFSLDFNFQLEDADGEPAGAGIGSAELAIPLALRQNDDG
                     SSSLVASYDRAIVKKMPISTMGFESGEQPGLEGALAILATRALQAQYPTTELLHFEPW
                     TIGGNDVKLAARKFAIFTESKVISLGMQTNLDLPPGISVKVGESLPMGVPMQIQMHPG
                     LLLAMSQRMIAEGVISRTYNDNGQPDPKGLYGVTLENLTANPIPGSNELDIGFRVWRT
                     QDGYCGFADAVTSLNLGIDQSNDSIKVTPTDELRVTGGEGIGALAEDNEELIEKNKKL
                     VDVFKQDLSEQVGITINYNEVGVAGSTILFDTLALNVEPNNIDIIIDFKVVATDG"
     gene            2178..2666
                     /locus_tag="VGB85_06100"
     CDS             2178..2666
                     /locus_tag="VGB85_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133629.1"
                     /translation="MSPTIPRPAFALALALCGATGLACTSDDLIAGTEVQTATTYVDP
                     TETFPFTTGEPEDSTSTGEMPSEQSCEQAVTCITKCAIELPMGVPEQDYSCFLPCVDG
                     MSTEQWLALIEIGECVYNFCTATAKCPDVDDEMTCTGCLLFSLQQDPGVMGCEMQAMA
                     CE"
     gene            2683..>3040
                     /locus_tag="VGB85_06105"
     CDS             2683..>3040
                     /locus_tag="VGB85_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133630.1"
                     /translation="MTNRNDSMTWTRALLSTAVLLAVATGAGESRAAPAETGATAGTT
                     STTGTGDAPAPAASTPAATTPGATTPATTTPATTTPDTTTPATTTPDTAAPSEPADAS
                     APAGPAPAPASEGPSAG"
BASE COUNT          483 a          966 c         1105 g          486 t
ORIGIN      
        1 cgcggatcgc caggaactcg cagccgacgg aggtcgggtt ggtctccgcg acctcgcaag
       61 ggccctcgca cttggcgatc gtgccctcct cgccgacctc ctcgttgcag gtctgatagt
      121 tcccgcagga cacgggctcc cactgcaggc cggtcggctt gcagatctcg cgggtcacgg
      181 agtcggcgca gcgctccgcg cccggctcgc acagcagcac gagctcgccg gtggtcgtca
      241 ccgggtcggt cgtgaccggg gtggtcgtct cgggcggggt gccggtagtg ctggtgtccg
      301 agctgctctc catcgccgtg ttgtcgccac aggcggcgag gacggaggcg gcgaggatgg
      361 aggtggcgag caggcgcgag cgggaggtgg cgagcagctg cgagcgggag gaggtcgtcg
      421 taaaaaagtt aaacataatc actccgtggg gcgcatctca gccgtcggtg gcgacgacct
      481 tgaagtcgat gatgatgtcg atattgttcg gctcgacgtt gagggcgagg gtgtcgaaca
      541 ggatcgtgct gccggccacg ccgacctcgt tgtagttgat cgtgatgccg acctgctcgc
      601 tgaggtcctg cttgaagaca tcgacgagct tcttgttctt ctcgatcagc tcctcgttgt
      661 cctcggcgag cgcgccgatg ccctcgccgc cggtgacgcg cagctcgtcg gtcggggtga
      721 ccttgatgct gtcgttgctc tggtcgatgc cgagattcag cgaggtgacg gcgtcggcga
      781 agccgcagta gccgtcctgg gtgcgccaga cgcgaaagcc gatgtcgagc tcgttgctgc
      841 cggggatcgg gttggcggtg aggttctcga gggtgacgcc gtagaggccc ttggggtcgg
      901 gctggccgtt gtcgttgtag gtccgggaga tcaccccttc ggcgatcatc cgctggctca
      961 tcgccagcag caggcccggg tgcatctgga tctgcatcgg cacgcccatc ggcagggact
     1021 cgccgacctt gaccgagatg ccgggcggga ggtcgaggtt ggtctgcatg ccgagcgaga
     1081 tcaccttgct ctcggtgaag atggcgaatt tgcgcgcggc gagcttgacg tcgttgccgc
     1141 cgatcgtcca cggctcgaag tgcagcagct cggtggtcgg gtactgggcc tgcagggcgc
     1201 gcgtggcgag gatcgccagg gcgccctcga ggcccggctg ctcgcccgac tcgaagccca
     1261 tcgtgctgat cggcatcttc ttgacgatgg cgcggtcgta gctggcgacc agcgaggagc
     1321 tgccgtcgtc gttttggcgc agcgccagcg ggatcgcgag ctcggccgag ccgatgccgg
     1381 cgcccgcggg ctcgccgtcg gcgtcctcga gctggaagtt gaagtcgagg ctaaacagta
     1441 cgcaactgct gcaattgggc accttgacga tctggatcgt cggcgtgccc tgcgggatga
     1501 agttgaggcc gtaggggccg atgtcgagct tgctggcgaa cgggacgttg ccgccgacga
     1561 ggccgttgag caggttctgg atgccggtct ccgacagcac catcgccatc ggatacagcg
     1621 aggactcgct gatgtccggc agcagcacgc cgtcgtcggc gaaggcgtcg gtgaacgcct
     1681 gttgctggct ggtgcagtcg agctcgatgt cctcgccgcg gcacgccggc accgcgaggg
     1741 acgccagaac cccggcgagc aggccgatac aggcggttcc gagggcgttc gttcgcttca
     1801 tggtcgatcc tcaagcaagg ggtcaggcaa agtcaggaag gggggacagg ccccgtaaac
     1861 gtggagtgac gcgggaagcg agccccccga cgacaaccct tagtatgacc cgtggcggcg
     1921 agaaaacaca aggctcgcgc tcgtgcgcgc atgtggccgg acccggccgg acccgggtcc
     1981 gaggggctcg tggtccaccg aagctggcaa aattgtcgtg atgctccagc gccaggcgta
     2041 cggacctcgg cacatgcggg gacgtgggcc gaaattggcc cggagccgca ggaaaaacat
     2101 cgcgggcgcg acgaagggaa acgtttgtgg tgcgggcggt cgctggtatc gtgcggcgga
     2161 tggtccgggc tcctcgcatg tctcccacga tcccaagacc ggcgttcgcg ctggccctgg
     2221 ctctttgcgg cgcgacgggg ctcgcgtgca cctcggacga cctgatcgca ggcaccgagg
     2281 tccagaccgc gaccacctac gtcgacccga cggagacctt tccgttcacg accggcgagc
     2341 ccgaagactc gacgagcacc ggcgagatgc cgagcgaaca gtcgtgcgag caggcggtga
     2401 cgtgcatcac caagtgtgcg atcgagctgc cgatgggcgt gccggagcag gactacagct
     2461 gcttcctgcc gtgcgtcgac gggatgagca ccgagcagtg gctcgcgctg atcgagatcg
     2521 gcgagtgcgt ctacaacttc tgtacggcga ccgcgaagtg cccggacgtc gacgacgaga
     2581 tgacctgcac ggggtgcctg ctcttcagcc tccagcagga ccccggcgtg atgggctgcg
     2641 agatgcaagc gatggcgtgc gagtgacgtg caaggccgcc acatgacgaa caggaacgac
     2701 tcgatgacct ggacccgcgc gctgctctcc actgctgtcc tgctcgccgt cgccaccggc
     2761 gccggcgagt cgcgtgccgc accggccgag accggcgcga ccgctggcac gacgagcacg
     2821 acgggcacgg gcgacgcgcc cgcgccggcg gcttcgaccc cggctgcgac caccccgggt
     2881 gcgacgaccc cggccacgac gaccccggcc acgacgaccc cggacacgac gaccccggcc
     2941 acgacgaccc cggacacggc tgcgccgagc gagccggccg atgcgagcgc gccggccggt
     3001 ccggcacctg cgccggcgag cgagggtccg agcgcgggtg
//
LOCUS       DASWNJ010000350         4566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_466108, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000350 DASWNJ010000000
VERSION     DASWNJ010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466108"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VGB85_06110"
     CDS             complement(<1..786)
                     /locus_tag="VGB85_06110"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY0133631.1"
                     /translation="MAIQGFDEQEFTHDGVTKTVYRKGTGPGVIVITEVPGIHPGVID
                     FATMLAGQGFSVALPELFGTTGKPVTIPYALGTMAKLCVSREFKLLAAHESSPITEWL
                     RALARKLHGELGGPGVGAIGMCLTGNFALSMAVEPSLMAPVLSQPALPLPVGKERAAS
                     LAVSPSELATIKRRVNDEGYKVLGLCFTADPKVPRARFETLKQELREGFEAIEIDSAP
                     GNANGIKKDAHSVLTIDLVRDAGHPTEQALQRVFAFLHERLDPA"
     gene            855..1223
                     /locus_tag="VGB85_06115"
     CDS             855..1223
                     /locus_tag="VGB85_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133632.1"
                     /translation="MTYEFYNVLHIVGIILVFMALGAALLHAGNGGTREQNPSRKLVA
                     GLHGLGLLLILIAGFGMLAKRQLMVGGLPGWIHPKLLVWLLLGAAPALIMRKPGSVSV
                     LWVVVPLLGAVAAYFGINHG"
     gene            complement(1332..1880)
                     /locus_tag="VGB85_06120"
     CDS             complement(1332..1880)
                     /locus_tag="VGB85_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133633.1"
                     /translation="MLRALVVCCVAMPLLACGGGAPKGPTAEQKQAEEAKKAEDAKNE
                     AGVAERKAKREAEVAAQAEAKTKVTAELERLTVLPEKLPKDPVAACEAVGKAHDEFVK
                     RTGDAKAIAAWDAGGKDKALPMTIVQCAQADSVKTAACQKNALDGAGPELKDETKTIM
                     QMCIDKFAAKKAPAAMPKRRPG"
     gene            2004..3719
                     /locus_tag="VGB85_06125"
     CDS             2004..3719
                     /locus_tag="VGB85_06125"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEY0133634.1"
                     /translation="MFGSLPLPPRRHIPADLDLCDLAVLTSCHDELATRPLVADDPWA
                     LEQWLRDWSELESLLSEEQTRREIAVTCHTDDPAIEAAYLDWVDRVAPHCKPLRDRLA
                     RRLVTHPGAAALDPARHRCLLRNLANQVDLYRDALVPLEAEQQRLCAEHQKLRGGMKA
                     DFDGELRTLEAIEAAEDHADPARREAAWRAVSGRFLADKPALDDLFDRLLDNRAAQTR
                     AAGFADYREYRFRQLARFDYGPADCLAFHAAIEAAVVPLACDLLAERAREAGRAQVRP
                     WDLQAPRAGRSALRPFSDADALVGGCTQVFTAVDPDFGQQFARLAGLGLLDLMSRPGK
                     APGGYQANLEASGLPFIFANAVGRHEDVLTLLHEGGHAFHALASAADPLIWNRSPPME
                     FCEVASMGMELMASQHLGAFYSPVELRQATAQQLESIVLFLPYMACVDAFQHWLYTDS
                     DAARDPAARDATWLALHRRFFPTVDWSDLEAERAALWQRKLHIFELPFYYVEYGIAQL
                     GALQVFAGYQRDPAATVAAYRGGLSLGARAGLRELFTAAGLRFDLGEEAVRACMDVVR
                     RALAA"
     gene            3832..>4566
                     /locus_tag="VGB85_06130"
     CDS             3832..>4566
                     /locus_tag="VGB85_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PA domain-containing protein"
                     /protein_id="HEY0133635.1"
                     /translation="MRRVPVALALLLACSRSPAPTPPPDGTGTAIQDPPADPWKDIHV
                     DVARIRGHLEYLADDARQGRAPGSEGDRESAQHVEAAFRELGLRPAFGGEYCQRFTVV
                     DGVRLLANQSAALRLGATDTAIELVPFTTATTAPVTAPLIYIGHGVADDPKLAKAMKG
                     AIVVARAGAPDDPHLDPTRTRAQSKLIAARDHGAIGFILWDPDSDLAFPNHGEANDLK
                     LPALAVGKAGTPALLAAFGVKTPAEGV"
BASE COUNT          586 a         1833 c         1444 g          703 t
ORIGIN      
        1 ggccggatcg agccgctcgt gcaggaacgc gaacacccgc tgcagcgcct gctcggtcgg
       61 gtgacccgcg tcgcgcacga ggtcgatcgt cagcacgctg tgggcgtcct tcttgatgcc
      121 attggcgttg cccggcgccg agtcgatctc gatcgcctcg aagccctcgc gcagctcctg
      181 cttcagggtc tcgaagcggg cgcgcgggac cttggggtcg gcggtgaagc acaggccgag
      241 caccttgtag ccctcgtcgt ttacgcggcg cttgatcgtc gccagctccg agggcgacac
      301 cgccagcgag gccgcgcgct ccttgccgac cggcagcggc agggccggct gcgacagcac
      361 cggggccatc agggacggct cgaccgccat cgacagcgcg aagtttccgg tcaggcacat
      421 cccgatcgcg ccgacgcccg ggccgccgag ctcgccgtgg agcttgcggg ccagcgcgcg
      481 cagccactcg gtgatcgggc tcgactcgtg ggccgcgagc agcttgaact cgcggctgac
      541 gcacagcttc gccatcgtgc ccagcgcgta ggggatcgtc accggcttgc cggtcgtgcc
      601 gaacagctcc ggtagcgcga cgctaaagcc ctggccggcg agcatcgtgg cgaaatcgat
      661 gacccccgga tggatcccgg gcacctcggt gatgacgatg actcccggcc ccgtaccctt
      721 gcgatacacc gtcttggtga cgccgtcgtg cgtgaactct tgctcgtcga acccttggat
      781 cgccatgcgc gaagtctacc aggggccgcg cacgtcttgt caacttcgtc cggagcgggc
      841 aacgtcccct cgccgtgacc tacgagtttt acaacgtcct ccacatcgtc ggcatcatcc
      901 tcgtgttcat ggcgctcggc gcggcgctgc tgcacgcggg caacggcggc acccgcgagc
      961 agaacccctc gcgcaagctc gtcgccggtc tgcacggcct cggcttgctc ctgatcttga
     1021 tcgccggctt cggcatgctc gccaagcgcc agctgatggt cggcggcctg cccgggtgga
     1081 tccaccccaa gctgctcgtg tggctgctcc tcggcgccgc cccggccctc atcatgcgca
     1141 agccgggctc ggtctccgtg ctgtgggtcg tcgtgccgct gctcggcgcc gtcgcggcct
     1201 acttcggtat caaccacggc tagcgacgca cggctagcga cgcacggctg gcgacgcacg
     1261 gctaacggcg cacgactggc ggcgcacggc tagcaggccg cgcaccgcgc ccgccggcgg
     1321 tgcgcgccgc ctcagcccgg acggcgcttg ggcatcgccg ccggcgcctt cttggccgcg
     1381 aacttgtcga tacacatttg catgatcgtc ttggtctcgt ccttgagctc cgggccggcg
     1441 ccgtcgagcg cgttcttctg acacgcggcc gtcttcacgc tgtcggcctg ggcgcactgc
     1501 acgatcgtca tcggcagcgc cttgtccttg ccgccggcgt cccacgccgc gatcgccttg
     1561 gcgtcgccgg tccgcttgac gaactcgtcg tgcgccttgc cgaccgcctc gcaggccgcg
     1621 accggatcct tgggcaactt ctccggcagc accgtgaggc gctcgagctc cgccgtcacc
     1681 ttggtcttcg cctccgcctg cgccgccacc tcggcctcgc gcttggcctt gcgctccgcg
     1741 acccccgcct cgttcttggc gtcctccgcc ttcttggcct cctccgcctg cttctgctcc
     1801 gcggtcgggc ccttgggcgc cccgcctccg caggccagca gcggcatggc gacgcagcac
     1861 acgacgagag ctcgcagcac ttttcgaccg ggatggcttc gcagcaaggg ctcacggtgc
     1921 cacggccata gcggcgccgc aaggtccgtc ggccgccgcg gacatcgttt atcgaggcac
     1981 ggcctcggtg ctacgctcgc gccgtgttcg gatcgctgcc gctcccgccc cgtcgccata
     2041 ttccggccga tctcgacctg tgcgacctcg ccgtcctgac ctcgtgtcac gacgagctcg
     2101 ccacccgccc gctcgtcgcc gacgacccct gggcgctcga gcaatggtta cgcgactgga
     2161 gcgagctcga gtcgctgctc tcggaggagc agacgcgccg cgagatcgcc gtcacctgtc
     2221 acaccgacga ccccgcgatc gaagccgcgt acctcgactg ggtcgaccgc gtcgccccgc
     2281 attgcaagcc gctgcgcgac cgcctcgcgc gccgcctcgt cacccacccc ggcgccgccg
     2341 cgctcgaccc tgcccgccat cgctgtctcc tgcgcaacct cgccaatcag gtcgacctct
     2401 accgcgacgc cctcgtcccg ctcgaggccg agcaacagcg cctgtgcgcc gagcatcaga
     2461 agctgcgcgg cggcatgaag gccgacttcg acggcgagct gcggaccctc gaggcgatcg
     2521 aagccgccga ggatcacgcc gaccctgcgc gccgcgaggc cgcctggcgt gcggtgtccg
     2581 gccgcttcct cgccgacaag ccggccctcg acgacctgtt cgaccgcctc ctcgacaacc
     2641 gcgccgccca gacgcgcgcc gccggcttcg ccgactaccg cgagtatcgc ttccgccagc
     2701 tggcccgctt cgattacggc cccgccgact gcctcgcctt ccacgccgcg atcgaagccg
     2761 ccgtcgtgcc gctcgcgtgc gacttgctgg cggagcgcgc ccgcgaggcc gggcgcgcgc
     2821 aggtgcgccc gtgggacctg caagccccgc gcgccggtcg gtccgcgctg cgtcccttca
     2881 gcgacgccga cgcgctcgtc ggcggttgca cgcaggtgtt caccgccgtc gaccccgact
     2941 tcgggcagca gttcgcccgc ctcgccgggc tcggcctgct cgacctcatg tcgcgccccg
     3001 gcaaggcccc cggcggctac caggccaacc tcgaggcctc gggcctgccc ttcatcttcg
     3061 ccaacgccgt cggccgccac gaggacgtcc tgaccctcct gcacgagggc ggccacgcct
     3121 tccacgcgct cgcctccgcc gccgacccgt tgatctggaa ccgcagcccg ccgatggagt
     3181 tctgcgaggt ggccagcatg ggcatggagc tgatggcgag ccagcacctc ggcgccttct
     3241 actcccccgt ggagctgcgc caggcgaccg cgcagcagct cgagtcgatc gtcctgttcc
     3301 tgccctacat ggcctgcgtc gacgcgttcc agcactggct ctacaccgac agcgacgccg
     3361 cccgcgaccc cgccgcccgc gacgcgacct ggctcgcgct gcaccgtcgc ttcttcccga
     3421 ccgtcgactg gtcggacctc gaggccgagc gcgccgccct gtggcaacgc aagctgcaca
     3481 tcttcgagct cccgttctac tacgtcgaat acggcatcgc ccagctcggc gccctgcaag
     3541 tgttcgccgg ctaccagcgc gacccggccg ccaccgtcgc tgcctaccgc ggcgggctgt
     3601 ccctcggggc ccgcgccggc ctgcgcgagc tgttcaccgc cgccggcctg cgcttcgatc
     3661 tcggtgaaga agccgtgcgc gcgtgcatgg atgtcgtgcg tcgagcgctc gccgcctgag
     3721 cggtccggct gcgggccgat ccccgagagt gcgaccctcg acgaagccct ccccgcatcg
     3781 cgagcagaac acccgtccca cctcgccgcc gtgctggcat actcccccgc gatgcgccgc
     3841 gtgccagtcg ccctcgccct cctcctcgcc tgctcccgct cgccggcccc gaccccgccc
     3901 cccgacggca ccggcaccgc gatccaggac ccgcccgcgg acccctggaa ggacattcac
     3961 gtcgacgtcg cccgaattcg cggacacctc gagtatctcg ccgatgatgc gcgtcagggt
     4021 cgggcgcccg gcagcgaggg tgaccgcgag tccgcgcagc acgtcgaggc cgcgtttcgc
     4081 gagctcggtc tacgccccgc gttcggcggc gagtattgcc agcgcttcac cgtcgtcgac
     4141 ggtgtgcgtc tgctcgcgaa tcagagcgcg gccctgaggc tcggcgccac cgacaccgcg
     4201 atcgagctcg tgcccttcac gaccgcgacc accgcaccgg tcaccgcccc gctgatctac
     4261 atcggccacg gcgtcgccga cgacccgaag ctggcgaagg cgatgaaggg cgcgatcgtc
     4321 gtcgcccgcg ccggcgcgcc ggacgacccg cacctcgacc ccacgcgcac gcgggcgcag
     4381 agcaagctga tcgccgcgcg cgaccacggg gccatcggct tcatcctctg ggatcccgac
     4441 agcgacctcg ccttccccaa ccacggcgag gccaacgacc tcaagctgcc cgccctcgcc
     4501 gtcggcaagg cgggcacccc ggccctgctc gccgcgttcg gcgtcaagac ccccgccgag
     4561 ggcgtg
//
LOCUS       DASWNJ010000351         2727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_147564, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000351 DASWNJ010000000
VERSION     DASWNJ010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147564"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VGB85_06135"
     CDS             <1..415
                     /locus_tag="VGB85_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133636.1"
                     /translation="AAPPRPPAALAPVSVAVPAADAPARVTTPETPIPPETPAPPRAA
                     RARAGGIDAEVALLDRARAALAAGSPEVALAWVDEHAREFPRGSLTDAREGTRVDALC
                     LQGRTLAAESAAQRLLAQRPDSPLTRRFENFRCDH"
     gene            455..1243
                     /locus_tag="VGB85_06140"
     CDS             455..1243
                     /locus_tag="VGB85_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133637.1"
                     /translation="MLSLAHRLALPLVLLALPACPVSEVVGDNPDETTTTATTADTGT
                     TDPGPTTEPTTEPTDPGDVTTGPGDPETGTTGPSQTATTDATETAGDTDTAGDTDTAG
                     DTDTAGPSPDEFVALEACDIVDVCPQWTQNFAESDAEGEPPQRLCVWEGLAAGTAGRY
                     RYVTEREFGNGHSQTTTIMNVHAERKVTFARHHVGYYEEGGGEQVKFNDYELAMTCTL
                     AAPELFESCPMVDMYDADCYWMSNPFDEGTPWFTDCVAQGPMCE"
     gene            1460..2662
                     /locus_tag="VGB85_06145"
     CDS             1460..2662
                     /locus_tag="VGB85_06145"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEY0133638.1"
                     /translation="MTRDPRAPSPGTLAVHGGEPRRLLGDAVVTPIVATSTYSFRDTA
                     ELRDHFEGTAPRDEYGRYGNPTVAIAERKLAALEAAEDCALFASGMAAITTTLFALLK
                     PGQHVVLTADGYRRTRQFITTFLARWGVEHTLVEPGDHAALAAAIVPGKTRVLLTESP
                     TNPYLRVADLRALAGLRAGHRGLKLVIDSTFATPINQQPLAQGADLVLHSCTKYLGGH
                     NDLLAGGVCGDAALVGAIRDLRGMFGGVLDPHAAYLLTRGLKTLELRVHRQNATGQRV
                     AEFLAGHPAVETVYYPGLPSHPDHEVARAHMRGFGGVVSFLVRGDEARTSRFIDRCQI
                     PKIAPSLGGVESLIEQPALMSFYELTSEQRLAVGIRDNLVRLSVGIEDPADLLADLDQ
                     ALAEPATP"
BASE COUNT          412 a         1070 c          900 g          345 t
ORIGIN      
        1 cgcggcgccg ccccggccgc ccgccgcgct cgccccggtg agcgtcgcgg tgccggccgc
       61 cgacgcgccc gcgcgcgtga cgaccccgga gacgccgata cccccggaga cgccggcgcc
      121 gccacgcgcc gcccgggcgc gcgcgggtgg catcgacgcc gaggtggcat tgctcgaccg
      181 tgcccgcgcc gcgctcgcgg ccggcagccc cgaggtcgcg ctcgcgtggg tcgacgagca
      241 cgcgcgcgag ttcccgcgcg gctcgctgac ggacgcgcgc gaggggaccc gggtcgacgc
      301 gttgtgtctg caaggtcgga cgctcgcggc cgagtccgcg gcgcagcgcc tgctcgccca
      361 gcgcccggac tcaccgctga cgcggcgctt cgaaaatttt agatgcgacc actgacggac
      421 cgccgacgcg gcggggaagc agggggttat gaccatgctt tctcttgctc atcgcctcgc
      481 gcttccgctc gtgctgctcg ccttgcccgc ctgccccgtg tccgaggtcg tcggcgacaa
      541 ccccgacgag acgaccacca ccgcgaccac cgccgacacc ggcacgaccg accccgggcc
      601 gaccaccgag ccgaccaccg agccgaccga ccccggcgac gtgacgacgg gcccgggcga
      661 tcccgagacg ggcaccaccg gaccgtccca gacggcgacg acggacgcca cggagaccgc
      721 gggcgacacc gacaccgccg gcgacaccga caccgccggc gacaccgaca ccgcggggcc
      781 gagccccgac gaattcgtgg cgctcgaggc gtgcgacatc gtcgacgtgt gcccgcaatg
      841 gacgcagaac ttcgccgaga gcgacgccga gggcgagccg ccgcagcggc tgtgcgtgtg
      901 ggaggggctc gcggccggca cggccggtcg ctaccgctac gtgaccgaac gcgagttcgg
      961 caacggccac agccagacga cgacgatcat gaacgtgcac gccgagcgca aagtgacctt
     1021 cgcgcgccac cacgtcggct attacgaaga gggcggtggg gagcaggtga agttcaacga
     1081 ttacgagctg gcgatgacct gcacgctcgc cgcaccggag ctgttcgagt cgtgcccgat
     1141 ggtcgacatg tacgacgccg attgctactg gatgtcgaac ccgttcgacg aggggacccc
     1201 gtggttcacg gactgcgtgg cccagggccc gatgtgcgaa tgacgcggcc tcgtcgtccg
     1261 cggctccggt gatcgaggac ccgcgcacaa atacaattca tgaaggacga cgcggacttg
     1321 tggcgaccgg cgggcgcgta caaggggtcg gagagtccgc gctgtacggc ctggagatcg
     1381 gacggatcgc tccggagcgc ccccgccggg tccagaaccg gcgaggacac gcccgcccgg
     1441 caccgctatc atgcgcgccg tgacgcgaga cccccgtgca ccatccccgg gaacgctggc
     1501 cgtgcacggc ggtgagccgc gacgcctgct gggcgacgcg gtcgtcaccc cgatcgtcgc
     1561 gacctccacc tacagcttcc gcgacaccgc cgagctgcgc gaccacttcg agggcaccgc
     1621 cccgcgcgac gagtacggcc gctacggcaa cccgaccgtg gcgatcgccg agcgcaagct
     1681 cgccgcgctc gaggccgccg aggactgcgc cctgttcgcc agcggcatgg cggcgatcac
     1741 caccacattg ttcgcgctgc tcaaacccgg ccagcacgtc gtgttgaccg ccgatgggta
     1801 ccgccgcacg cgtcagttca tcaccacctt tctggcccgc tggggcgtcg agcacacgct
     1861 cgtcgagccc ggcgatcacg cggcgctcgc cgccgcgatc gtgcctggca agacccgcgt
     1921 gctgctgacc gagtcgccga ccaacccata cctgcgcgtc gccgacctgc gcgcgctcgc
     1981 gggcctgcgc gctggccacc gcggcctcaa gctcgtcatc gacagcacct tcgccacgcc
     2041 gatcaatcaa cagccgctgg cgcagggcgc cgacctcgtg ctgcactcgt gcaccaagta
     2101 cctcggcggc cacaacgacc tgctcgccgg cggtgtgtgt ggcgacgccg cgctcgtcgg
     2161 cgcgatccgt gatcttcgcg gcatgttcgg cggcgtgctc gacccgcacg ccgcctacct
     2221 gctgacccgc ggcctcaaga ccctcgagct gcgcgtgcat cggcaaaacg ccaccggcca
     2281 gcgcgtcgcc gagttcctcg ccggccaccc cgcggtcgag accgtctact accctggcct
     2341 gccgtcgcac cccgatcacg aggtcgcccg cgcgcacatg cgcggcttcg gcggcgtcgt
     2401 cagcttcctc gtgcgcggcg acgaggcccg caccagccgc ttcatcgacc gctgccagat
     2461 cccgaagatc gccccctcgc tcggcggcgt cgagagcctg atcgagcagc cggccctgat
     2521 gtccttttat gagctgacga gcgagcagcg cctcgccgtc ggcatccgcg acaacctcgt
     2581 gcgcctcagc gtcggcatcg aggaccccgc cgacctgctc gccgacctcg accaggccct
     2641 cgccgaacca gccacgccat gacccgactc cgcgtcctca agttcggcgg ctcctccgtc
     2701 ggcgccaccg accgcctcgt ccgcgtc
//
LOCUS       DASWNJ010000352         3264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_112703, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000352 DASWNJ010000000
VERSION     DASWNJ010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112703"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VGB85_06150"
     CDS             complement(<1..257)
                     /locus_tag="VGB85_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133639.1"
                     /translation="MTDDGALSSRAGDAARSRGQARACGAAEREAASCASNAGGDTVE
                     GVPDISAVVSPLIDLLRPLVGQAVRAFFGTAVGMMCLGLVLG"
     gene            270..1214
                     /locus_tag="VGB85_06155"
     CDS             270..1214
                     /locus_tag="VGB85_06155"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY0133640.1"
                     /translation="MSSAEDQALVELGRMLRASGYRFVTPTPATHQRVNARPSNREAR
                     SLTDVLGWSRPFRSGVVPAEIEKLLAAAGALERVGDRSRSTVRFSTVDPADESDSPAI
                     HVHSAFPTTGADAVFFGPDTYRFIQLLRRSVGPARRLVDIGAGSGAGGLALRERMQQV
                     VLADINHAALRHARINATLAGARAGDVEVVESDVLAGVTGPIDVVIANPPYLADPGRR
                     LYRDGGGALGFDLSVRIVHEALARLGSGGQLVLYTGSPVVAGQHPLRASLAPLLAARP
                     CRSSWEEIDPDVFGEELDAPAYQDVERIAVVALVVHVA"
     gene            1235..2125
                     /locus_tag="VGB85_06160"
     CDS             1235..2125
                     /locus_tag="VGB85_06160"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEY0133641.1"
                     /translation="MLHSASTAHSDLELQRRLNSFNHERLRPRLPVDLDTADDDLDLD
                     TEVQLRGLEEHFIGSERAAVAAAAAEAPTDPEGFVAWFEALQERGPGQGDPLFPWLAE
                     TATLEQLRWFLAQEVAGEAGFEDLVALTQLKFPVQAKLELARNFWDEMGRGKTSGMHG
                     PMLDRLARSLDLNDSPAEIVAEALALGNLMIALATNRRYAYHSVGALGVIELTAPGRA
                     ACVNEGLRRLGVHASDRQYFALHATLDVQHSIAWNREVLLTLAETGPGTVRAMAEGAL
                     LRLTAGARCFARYRRELGLS"
     gene            2246..>3264
                     /locus_tag="VGB85_06165"
     CDS             2246..>3264
                     /locus_tag="VGB85_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133642.1"
                     /translation="MRFAAFVAPYLLDATVRFIDNAARLPGVRLALITSEPAERLPPR
                     LRESIAAHWRVDDALDPQQIVDAVRGLSAHHGGRRIERMIAVLEQLQVPVAEARDFLG
                     IPGMNAATAANFRDKHRMKDVLRAAGVPVARHRLCHAPAEAVAFAGEVGYPIVAKPPA
                     GAGAVDTFQLEDSGALHRWLWQHTPTPDGPWLLEEFLTGDEFTFDSVMIDRRVVWHSI
                     ARYMPTPLEALRNPWIQWVVLLPRDISGAAYEPIGHVAPAALRALGLETGLSHMEWFR
                     RPDGSVAVSEVGARPPGAGISSALGYAHDDDLYAFWPRLMLLDQFNPPARRWSVGVAY
                     LRGEGT"
BASE COUNT          425 a         1298 c         1079 g          462 t
ORIGIN      
        1 ccgagcacga ggccgaggca catcatcccg acggcggtcc cgaagaaggc ccgcaccgcc
       61 tggccgacca gcggccgcag caggtcgatc aggggggata cgacggcgct gatgtccggc
      121 acgccctcga cggtatcacc acccgcgttg cttgcgcagc tcgccgcttc gcgctccgct
      181 gccccgcacg cgcgcgcctg cccccgcgat cgcgccgcat ccccggcccg gctcgacaac
      241 gcgccgtcgt cggtcacgat gggcgccacg tgtcatctgc agaggatcag gcgctcgtcg
      301 agctcggcag gatgttgcgc gcgtcgggct atcgcttcgt gacgccgaca cccgccaccc
      361 atcaacgcgt caacgccagg ccgagcaacc gtgaggcgcg ctcgctgacc gacgtgctcg
      421 gctggtcgcg gccgtttcgc agcggcgtcg tgccggccga gatcgaaaag ctgctggccg
      481 ccgccggtgc gctcgagcgc gtcggagatc gctcgcgcag cacggtccgc ttctcgaccg
      541 tcgaccccgc ggacgagtcc gacagccctg cgatccacgt gcactcggcc ttcccgacca
      601 ccggcgccga cgcggtgttc ttcgggcccg acacctatcg cttcatccag ctgctgcggc
      661 gatcggtcgg gcccgcgcgg cgcctcgtcg acatcggcgc cggcagcggc gccggcgggc
      721 tcgcattacg cgagcgcatg cagcaggtcg tgctcgccga catcaaccac gccgccctgc
      781 gtcacgcccg gatcaacgcc acgctggccg gcgcgcgcgc cggggacgtc gaggtcgtcg
      841 agagcgacgt gctcgcgggc gtcactggcc cgatcgacgt cgtcatcgcc aacccgccgt
      901 acctcgccga ccccgggcgc cgcctgtacc gcgacggcgg tggggctctc ggcttcgacc
      961 tctcggtgcg catcgtccac gaggcgctcg cgcggctcgg gtccggcgga cagctcgtgc
     1021 tgtacaccgg ctcgcccgtg gtcgccgggc agcacccgct gcgcgccagc ctcgcacctc
     1081 tgctcgcggc gcgtccgtgt cgctcctcat gggaggagat cgatcccgat gtcttcggcg
     1141 aggagctgga cgcaccggcc tatcaggacg tggaacgcat cgccgtcgtc gccctcgtcg
     1201 tgcacgtcgc ttagagcgcg gttacacccc tcgcatgctt cactcggcga gcaccgccca
     1261 cagcgacctc gagctgcaac gccggctcaa ctcgttcaac catgagcgcc tgcggccgcg
     1321 cctccccgtc gacctcgata ccgccgacga tgacctcgac ctcgacaccg aggtccagct
     1381 gcgtggactc gaggagcact tcatcggcag cgagcgggcc gccgtcgctg ccgcggccgc
     1441 cgaggcgccg accgaccccg agggcttcgt cgcgtggttc gaggccctgc aagagcgcgg
     1501 ccccggccag ggcgacccgc tgtttccgtg gctcgccgag accgcgacgc tggagcagct
     1561 gcgctggttc ctcgcccagg aggtcgccgg tgaggccggc ttcgaggacc tcgtcgcgct
     1621 gacgcagctc aagttcccgg tgcaggccaa gctcgagctg gcgcgcaact tctgggacga
     1681 aatgggtcgc ggcaagacct ccggcatgca cggcccgatg ctcgaccgtc tggcgcgatc
     1741 gctcgacctc aacgactcgc cggcggagat cgtcgccgag gcgctcgcgc tcggcaacct
     1801 gatgatcgcg ctcgcgacca accggcgcta cgcctaccac tcggtcggcg ccctcggggt
     1861 gatcgagctg accgcccccg ggcgggccgc gtgcgtcaac gagggcctgc gccgcctcgg
     1921 cgtccacgcc tccgaccgcc agtacttcgc cctgcacgcg accctcgacg tgcaacactc
     1981 gatcgcctgg aaccgcgagg tcctgctgac gctcgccgag accggaccgg gcaccgtgcg
     2041 cgcgatggcc gagggcgccc tgctgcgcct gaccgccggc gcccgctgct tcgcacgcta
     2101 ccgccgcgag ctcggcctgt cctgagcgtt cgcgcggggc gcggactccc ggggccgccg
     2161 cgagcatgcg cgcccccggg ccctcgacat cgagctctgg cgcgaagtcg cggccgcgta
     2221 ctacgattgc cgcaggttca acgccgtgcg cttcgccgcc ttcgtcgccc cctatctgct
     2281 cgacgcgacc gtccgcttca tcgacaacgc cgcgcgcctg cccggggttc gcctcgcgct
     2341 gatcaccagc gagccggccg agcgcctgcc cccgcgcctg cgtgagagta tcgccgcgca
     2401 ctggcgcgtc gacgacgccc tcgacccgca gcagatcgtc gacgcggtgc gcggcctgtc
     2461 ggcgcaccac ggcggccggc gcatcgaacg aatgatcgcc gtgctcgagc agctgcaggt
     2521 ccccgtcgcc gaggcccgcg acttcctcgg catccccggc atgaacgccg cgaccgccgc
     2581 caacttccgc gacaagcacc gcatgaagga cgtcctgcgc gccgccggcg tgccggtcgc
     2641 ccgccatcgc ctgtgccacg cgcccgccga ggcggtcgcc ttcgcgggcg aggtcggcta
     2701 cccgatcgtc gccaagccgc ccgccggcgc cggtgcggtc gacaccttcc agctcgagga
     2761 cagcggcgcg ctgcatcgct ggctctggca acacacgccg acgcccgacg gcccgtggtt
     2821 gctcgaggag ttcctcaccg gcgacgagtt caccttcgac agcgtgatga tcgaccgcag
     2881 ggtcgtgtgg cactcgatcg cccgctacat gccgaccccg ctcgaggccc tgcgcaaccc
     2941 gtggatccag tgggtcgtgt tgctgccgcg cgacatctcc ggcgccgcct acgagccgat
     3001 cgggcacgtc gcccccgcgg ccctgcgcgc gctcggcctc gagaccggcc tgtcgcacat
     3061 ggagtggttt cgccggcccg acggcagcgt cgcggtctcc gaggtcggcg cccgcccgcc
     3121 cggcgccggc atcagctccg ctctcggcta cgcccacgac gacgacctgt acgcattctg
     3181 gccgcgtctg atgctgctcg accaattcaa cccgcccgcg cgccgctggt cggtcggcgt
     3241 cgcctacctg cgcggcgagg gcac
//
LOCUS       DASWNJ010000353         1708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_218479, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000353 DASWNJ010000000
VERSION     DASWNJ010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218479"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VGB85_06170"
     CDS             <1..240
                     /locus_tag="VGB85_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibro-slime domain-containing protein"
                     /protein_id="HEY0133643.1"
                     /translation="TYTGGETFTFTGDDDVWVFINKKLVIDLGGVHGNTGGNVALDTL
                     GLSMGNTYTLDVFHAERHTVQSNFRIDTTICAVPQ"
     gene            complement(257..556)
                     /locus_tag="VGB85_06175"
     CDS             complement(257..556)
                     /locus_tag="VGB85_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133644.1"
                     /translation="MNAGTLLILAALAASLLLLVRTRSRLWPAIATAASALMALFALG
                     LVSFAVKGVPLGLLLAATLTAAGVMAWLGSGGKLPVTAATVVIVVGAAQLLSAVL"
     gene            complement(644..1615)
                     /locus_tag="VGB85_06180"
     CDS             complement(644..1615)
                     /locus_tag="VGB85_06180"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0133645.1"
                     /translation="MNSRPDFAAIEAFARVAETGSFRAAALALAAPVSTVSVQVSRLE
                     ARLGVRLLERTTRRVSLTSEGRAYFEQVRAALDVMATAEHSVTGRSGEARGRLRIAAP
                     VELGQAVLGRVLADYMQAHPDVALELELGNVHVDPIRDGFDVVLQTDPADSSSVVARK
                     LGAPSRYRLVASAAYLARHGAPEHPRELARHTCLVMGTRGEATTWRFARAPAIVHRHA
                     TANSWALLRDLAAAGHGIARLPNFLAAPAIARGEVEAVLDPFAPTAEQMYAVYSRGRH
                     VPARVSAFVAALQSFLKVWPGCLSSPRRPVEGRGARGAASGEARARA"
BASE COUNT          277 a          613 c          599 g          219 t
ORIGIN      
        1 acctacaccg gcggcgagac cttcaccttc accggcgacg acgacgtctg ggtgtttatc
       61 aataaaaagc tcgtcatcga cctcggcggc gtgcacggca acaccggcgg caacgtggcc
      121 ctcgacactc tcggcctgag catgggcaac acctacactc tcgatgtatt ccacgccgaa
      181 cgccacaccg tgcagtcgaa ctttcgcatc gacacgacga tctgcgccgt gccgcagtga
      241 tacggcaact gacgctttac aggacagccg aaagcagctg cgccgcgccg acgacgatca
      301 ccaccgtcgc cgccgtgact ggcagtttgc cgcctgagcc gagccaggcc atcacgccgg
      361 cggcggtcag cgtcgcggcg agcagcagcc cgagcggcac gcctttcacc gcgaaggaga
      421 ccagtccgag ggcgaagagg gccatgaggg cgctcgcggc ggtggcgatc gccggccaca
      481 ggcgcgagcg cgtgcgaacc agcagcagca gcgaagccgc aagcgcggcg aggatcagca
      541 aggtacccgc gttcatctcg ccaccttacc gcatcccctc agccgatcgg ccgtcagcat
      601 cggcgaggag caacccacgg acccgcacga cgaaagcctt cgctcaggcc cgcgctcgcg
      661 cctcgccgct cgctgccccc ctggcacccc gcccctcgac cggccgccgc ggcgacgaca
      721 ggcagcccgg ccacaccttc aaaaagctct gcagcgcggc cacgaaggcg ctgacccgcg
      781 ctggcacgtg gcggccgcgc gagtacacgg cgtacatctg ctcggcggtc ggggcgaagg
      841 ggtcgagcac tgcctcgacc tcgccacgtg cgatcgcggg ggcggcgagg aagttcggga
      901 ggcgggcgat gccgtggccg gcggccgcga ggtcgcgcag cagcgcccag ctgttggcgg
      961 tcgcgtggcg gtgcacgatc gccggggccc gggcgaagcg ccaggtcgtc gcctcaccgc
     1021 gcgtgcccat caccaggcag gtgtgtcggg ccagctcgcg cgggtgctcc ggcgcgccgt
     1081 gacgcgcgag gtaggccgcg ctggcgacca gccgatatcg tgacggcgcg ccgagcttgc
     1141 gtgcgaccac cgaggagctg tccgcggggt cggtctgcag cacgacgtcg aagccgtcac
     1201 ggatcgggtc gacgtgcacg ttgccgagct cgagctcgag cgcgacgtcc gggtgggcct
     1261 gcatgtagtc cgcgagcacc cggccgagca cggcctgccc gagctccacc ggcgcggcga
     1321 tcctcaggcg cccgcgggcc tcgccgctgc gaccggtgac gctgtgctcc gccgtcgcca
     1381 tcacgtcgag cgcggcgcgg acctgctcga agtacgcgcg gccctcgctg gtcaggctga
     1441 cccgtcgggt ggtgcgctcg agcaggcgaa cgccgaggcg cgcctcgagc cgcgagacct
     1501 gcacgctgac ggtcgagacg ggggccgcga gggcgagcgc ggcggcgcgg aagctgccgg
     1561 tctcggcgac gcgggcgaag gcctcgatgg cggcgaagtc gggccgactg ttcatggcga
     1621 tgaacacagt gaacaatctt ccgggattgt tcaagtgccg cggctctcgt agaccgttcg
     1681 catgacgatc cgactgtatt acaccccg
//
LOCUS       DASWNJ010000354         5166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_359169, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000354 DASWNJ010000000
VERSION     DASWNJ010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359169"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="VGB85_06185"
     CDS             complement(<1..892)
                     /locus_tag="VGB85_06185"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0133646.1"
                     /translation="MEAEPETKVLPPGHPEASTSSAELVVARQSVGRYELVQRLGHGG
                     MATVYLGRVTGSAGFEKLVAVKVIHPHLAAEPEFVDMFLEEARIAAKIQSPHVAGILD
                     LGQDDGLHFMVMEYIDGETVSALLRQLRPRDERLPLPVVLQVLMDACEGLTAVHELRD
                     PDGNPYGLVHRDMSPQNLMVGFDGWTKIVDFGLVKATGKRHTHTGHLRGKLAYMSPEQ
                     ARGKPLTASADLFALGVVLWELLTGKRLFAGDSDAETLDRGTRCELPSLGAARPDLPP
                     EIEPILHRALAPDPADRDPSA"
     gene            1458..1685
                     /locus_tag="VGB85_06190"
     CDS             1458..1685
                     /locus_tag="VGB85_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133647.1"
                     /translation="MAKRQTRRSISVKGITYQRLKDFCESSGLSVSGYLEDIIAEKLD
                     AAGVPVPTVVKPRPTSKLVDADDIVSQHFTF"
     gene            2051..3697
                     /locus_tag="VGB85_06195"
     CDS             2051..3697
                     /locus_tag="VGB85_06195"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY0133648.1"
                     /translation="MTKKYLKSARAELSRRRAMQAMGSFIGTVALGCGDSSSGSGDGS
                     SSTGGGDGSSGDDTPTTSGGGGDGSSSGEAPTTSGGSGDGSSSGGDGTTSTTGTTGDE
                     TTTGSPVDDCSVQSDLTPEELLAGIDHIVVLMMENRSFDHYFGSLKVQEGQAINGLTG
                     DESNLNLDLLPVPVFVMDNFEPPDPPHGWVSCHNQFNLGENNGFVFENEKDHPGFGGL
                     AMGYHIREHLPVLYGLADNYTLCQRWYASVMGPTWPNRYYMHACTSDGGKTNFPKPFL
                     KTLWHACDEAGITSRMYFGDVPWVTGAFPLVPTVWSKLADDYNGFSFDSLSNPNNMQR
                     FFDDCAAGNLPNFTVIDPGFTSNDDHPSHNIQLGQILIGTIFKTLAASPIWNKTLFII
                     TYDEHGGFYDHVPPPKTIDAEPEFEQMGFRVPALVIGPTVRRGCASDMQFEHCSIGAT
                     LMRRFGIAPLNQRMAEAADVSSCINPDFIADPQPAPQIPLVNASVSQILAQVGHTTSQ
                     EELFLAAGVTLDEAFRKKVQADTLRLLAQAQKLGVARLRP"
     gene            3732..>5166
                     /gene="dnaX"
                     /locus_tag="VGB85_06200"
     CDS             3732..>5166
                     /gene="dnaX"
                     /locus_tag="VGB85_06200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY0133649.1"
                     /translation="MAYVVLARKYRPQRFADLVGQEHVTRTLANAIAHNRVHHAYLFC
                     GGRGLGKTTAARLLAKCLVCVNGPTIEACNVCHECVAVTEGRSVDVIEIDGASNNKVE
                     DVRNIREQVRYLPQTARRKIYIIDEVHMLTGSAFNALLKTLEEPPEHVTFIFATTEVH
                     ELPATILSRVSRFDFRRLSSAQLVGHLQNILRSEGVSVEEAGLYTVARAGDGSVRDSL
                     TLLDKVIAFASDTNKIGAAEVRVVLGVPGALAVAGLVEAVLARDATLTMRRFDEVFAS
                     GQDLLALALEFLKHLRDLMIVKLCGTRDVLVDASDAEYELLTQQASGVDSVVLAQLFD
                     RFTRVVDALPRTRVPRLVLEMGLLDLVHSEPLVPLGDLIDRLEQISSGAPGTGGGGSR
                     SLPPTARPSPPASAPARTRADANPGPPGPSPSQQPNPPSPSPPPSASQHSSPGQQSGR
                     APVDESSPYAKLLAPLLAGGGGAEAAKP"
BASE COUNT          833 a         1726 c         1794 g          813 t
ORIGIN      
        1 cggcggaggg gtcgcggtcg gcggggtcgg gcgcgagcgc gcggtgaagg atcggctcga
       61 tctcgggcgg caggtccggg cgcgccgcgc cgagcgacgg cagctcgcag cgggtcccgc
      121 ggtcgagcgt ctcggcgtcg ctgtcgccgg cgaacaggcg cttgccggtc agcagctccc
      181 acagcaccac gccgagcgcg aacaggtcgg ccgaggcggt cagcggcttg ccgcgcgcct
      241 gctccgggga catgtacgcc agtttgccgc gcaggtgtcc ggtatgcgtg tggcgcttgc
      301 cggtcgcctt gacgagccca aagtcgacga tcttggtcca gccgtcgaag ccgaccatca
      361 ggttctgcgg cgacatgtca cggtgcacga ggccgtaggg gttgccgtcg gggtcgcgca
      421 gctcgtgtac ggcggtgagg ccctcgcagg cgtccatcag cacttgcagg acgaccggca
      481 gcggcaggcg ctcgtcgcgg gggcgcagct ggcggagcag cgccgacacg gtctcgccgt
      541 cgatgtactc catgaccatg aagtgcaggc cgtcgtcttg gccgagatcg aggatgccgg
      601 cgacgtgcgg gctctggatc ttggcggcga tgcgcgcctc ctcgaggaac atgtcgacga
      661 actcgggctc cgcggcgagg tgcggatgga tgaccttgac cgcgacgagc ttctcgaagc
      721 cggcgctgcc ggtgacacgg ccgagataca cggtcgccat gccgccgtga ccgaggcgct
      781 ggaccagctc gtagcggccc accgactggc gggcgacgac cagctcggcg ctgctcgtgg
      841 atgcctccgg atggccgggg ggcagcacct ttgtctcggg ttcggcctcc atggtgaagc
      901 ggcccgctct tgtggggccc gatcttcacc atacgtcaaa gctcgcggcc gatcatccgc
      961 cgcgggaggt cgcagcgggc cgctcctggg ccttgcagac ctctgaccgg tacgccgacc
     1021 ggtgggtttc gttgggaatg gttgagccgt gagccgaccg ggtcgtgcct ggcgggaggc
     1081 cgcagaaatt tcctggcttc tcaaatcagt gatttttctg tatttcagct cactctcttt
     1141 gcacgtggcc tggatccgcg gtgaaggcgc gggtcctcca agctggatac aaaaccagga
     1201 agagatctca gagatctgct cgatgtcgaa aaatacatag gatctaccat gccacgtgtc
     1261 gcctgattca ccattgagcg ccctggccgg aaatcccgaa tgttggtttg atatcgaggg
     1321 cttgagcgaa cggatgcaca acttggtcac tgcgcgcgag ctacgcgacc tggggtaatg
     1381 tggaatgggc cttgcgtgtg gagttgggtt gacgtagagg gcaggagagc aggagctagc
     1441 ggcgaaggga ggacaccatg gcgaagcgac agacccgcag gagcatctcg gtcaagggca
     1501 tcacctatca gcgtctcaag gatttctgcg agtcctcggg gctcagcgtg agcggctatc
     1561 ttgaggacat tattgcggaa aagctggacg ccgccggtgt cccggtgccg acggtcgtca
     1621 agccgcgccc gacctcgaag ctggtcgacg cggacgacat cgtctcacaa cactttacgt
     1681 tctaagtcag cgcttccgcg ctgattggct gagtcagcgt ttcgcgcgga cagtcagcgt
     1741 ttcgcgcgga cagccggcgt tccagcgcgg acagtcagcg tttcgcgcgg gacggtccag
     1801 tcagcgcccc gtgccggtcg agtcggcgac ggatccatcg agtctcgacc ccgggctggg
     1861 tgcgccgggg ttttgggctc gagcttgcgc gtctgccttt gcgcctcatg aagaatgtgc
     1921 gcgatccggg tgcgtcgtgg gcacgggcac ggccgattct tcggggcgag tgcgaaagct
     1981 cgagtcgccg ccccgggcgc cggggtgggt gcgaatggag gggcggggcc gacctggtag
     2041 attggttgcg atgaccaaga agtacctgaa gtcggcgcgg gccgagctgt cacgtcggcg
     2101 ggcgatgcag gcgatgggct ccttcatcgg gaccgtcgcg ctcggctgcg gcgactcgtc
     2161 ttcgggtagc ggcgacggca gcagcagcac cggcggcggc gacggcagca gtggcgacga
     2221 cacgccgacg accagcggcg gcggcggcga cggcagcagc agcggtgagg cgccgacgac
     2281 cagcggcggc agcggcgacg gcagctcgag cggcggcgac ggcaccacga gcacgacggg
     2341 caccacgggc gacgagacga cgacgggctc gccggtggac gactgctcgg tgcagagcga
     2401 cctgacgccg gaggagctgc tggcgggcat cgatcacatc gtcgtcctga tgatggagaa
     2461 ccgcagcttc gaccactact tcggctcgct caaggtgcag gagggtcagg cgatcaacgg
     2521 tcttacgggt gacgagtcga acctcaacct cgacctgttg ccggtgccgg tgttcgtgat
     2581 ggacaacttc gagccgcccg atcctccgca cgggtgggtg agctgtcaca accagttcaa
     2641 cctcggcgaa aacaacggct tcgtgttcga gaacgagaag gaccacccgg gcttcggcgg
     2701 gctggcgatg ggctatcaca tccgcgagca cctgccggtg ctctacgggc tcgcggacaa
     2761 ctacacgctg tgtcagcgct ggtacgcatc ggtgatgggc ccgacctggc ccaaccgcta
     2821 ctacatgcac gcgtgcacct cggacggcgg caagaccaac ttcccgaagc cgttcctcaa
     2881 gaccttgtgg cacgcctgcg acgaggcggg catcacgagc cgcatgtact tcggcgatgt
     2941 cccgtgggtg acgggcgcct tcccgctggt cccgacggtg tggagcaagc tcgcggacga
     3001 ctacaacggc tttagcttcg actcgctgag caaccccaac aacatgcagc ggttcttcga
     3061 cgactgtgct gcgggcaacc tgcccaactt cacggtcatc gaccccggct tcacctcgaa
     3121 cgacgaccac ccgtcacaca acatccagct cggccagatc ctcatcggca cgatcttcaa
     3181 gacgctcgcc gcgagcccga tctggaacaa gaccctgttc atcatcacct acgacgagca
     3241 cggcggcttc tacgaccacg tgcccccgcc gaagacgatc gacgcggagc cggagttcga
     3301 gcagatgggc ttccgtgtgc cggcgctcgt catcggtccg acggtccgcc gcggctgcgc
     3361 gagcgacatg cagttcgagc actgctcgat cggcgcgacg ctgatgcggc gcttcggcat
     3421 cgcgccgctg aatcaacgca tggccgaggc cgccgacgtc tcgtcgtgca tcaaccccga
     3481 cttcatcgcc gacccgcagc cggcccccca gatcccgctg gtcaacgcgt cggtctcgca
     3541 gatcctcgcg caggtcggtc acacgaccag ccaggaggag ctgttcctcg cggcgggcgt
     3601 gacgctggac gaggccttcc gcaagaaggt ccaggccgac acgctgcggc tgctcgcgca
     3661 ggcgcaaaag ctgggcgtcg cccgcctgcg cccgtgaagc gcgggcgggc tcgtgctagg
     3721 gtgcggctcc cgtggcctac gtcgtcctcg cacgcaagta ccgcccgcag cgcttcgcgg
     3781 acctggtcgg ccaggagcac gtcacgcgca ccctggccaa cgcgatcgca cacaaccggg
     3841 tgcaccacgc gtacctgttc tgcggcggcc gcggcctcgg caagaccacc gcggcgcgcc
     3901 tgctcgccaa gtgcctggtg tgcgtcaacg gaccgacgat cgaggcgtgc aacgtgtgcc
     3961 acgagtgcgt cgcggtcacc gagggccgca gcgtcgatgt gatcgagatc gacggcgcga
     4021 gcaacaacaa ggtcgaggac gtccggaaca tccgcgagca ggtgcgctac ctgccgcaga
     4081 ccgctcgccg caagatctac atcatcgacg aggtccacat gctcaccggc tcggcgttca
     4141 acgccctgct gaagacgctc gaggagccgc cggagcacgt caccttcatc ttcgccacca
     4201 ccgaggtcca tgagctgccg gcgacgatcc tgtcgcgcgt gtctcgcttc gactttcgcc
     4261 ggctgtcgag cgcgcagctg gtcgggcacc tgcagaacat cttgcgcagc gagggcgtca
     4321 gcgtcgagga ggccgggctc tacacggtcg cgcgcgccgg cgacggctcg gtgcgcgact
     4381 cgctgacgct gctcgacaag gtgatcgcgt tcgccagcga caccaacaag atcggcgccg
     4441 ccgaggtccg cgtcgtgctc ggggtcccgg gggcgctcgc cgtcgccggc ctcgtcgagg
     4501 cggtgctcgc ccgcgacgcg accctgacga tgcgccgctt cgacgaggtg ttcgcgtcgg
     4561 ggcaagatct cctcgccctc gcgctcgagt ttctcaagca cctgcgcgat ctgatgatcg
     4621 tcaagctgtg cgggacccgg gatgtgctgg tcgacgcgtc ggatgccgag tacgagctgc
     4681 tgacgcagca ggcctccggc gtcgacagcg tcgtgctcgc gcagctgttc gaccgcttca
     4741 cgcgggtcgt cgacgccctg ccacggaccc gggtgccgcg cctcgtgctc gagatgggcc
     4801 tgctcgactt ggtccacagc gagcccctcg tgccgctggg cgacctgatc gatcgcctgg
     4861 agcagatctc gtccggcgcg cccggaaccg gcggcggtgg ctcccgatcg ctccccccga
     4921 ctgcccgtcc gtcgcccccg gcgagcgccc cggcgcgaac gcgcgcggac gcgaacccgg
     4981 gaccgccggg cccgagtccg agccagcaac cgaacccgcc gagtccgagc ccgccgccga
     5041 gcgcgagcca gcattcgagc ccgggccagc agtcgggccg cgcgccggtc gacgagagct
     5101 cgccgtacgc gaagctgctc gcgccgctgc tcgcgggtgg cggcggggcc gaggcggcga
     5161 agcccg
//
LOCUS       DASWNJ010000355         4512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_676967, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000355 DASWNJ010000000
VERSION     DASWNJ010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676967"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="VGB85_06205"
     CDS             <1..1102
                     /locus_tag="VGB85_06205"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY0133650.1"
                     /translation="GATVATTATTTTDPTTATTTTGDDTTGAAEAGTGGTTGGTTTDP
                     DTTTGEDTTGGAVSEPACLFEPFVNKGALAIDYAQFGPVIGSHCKGTNHQDITDIERV
                     VFLGDSVTVGTPPTPGDASYRAELADVLVDRFGLTPPNGLWKQYDPFNGVSVVKDAGD
                     FSSCSEWGARTDDFLAPGTQVDDCFPPDKLDKRTLVVFTMGGNDLAAIAKDGANGKPY
                     DEVVADAEKALLLMREAVQWFVDDPDKFPNGVFVVFANIYEFTDGTGDLMSCPAAGLG
                     GFDKPWEDVEALKQLVLWINEQVMDIAVSTQTDMIFMLENFCGHGFRAGDPTAPCYRG
                     PDSPNYFDVSCIHPTPAGHDVIKDLFTAVIDE"
     gene            1313..3046
                     /locus_tag="VGB85_06210"
     CDS             1313..3046
                     /locus_tag="VGB85_06210"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEY0133651.1"
                     /translation="MTAVSRLIPPYHPPGQPEPQEILDVMQQELRRSLDGLIVPGSPR
                     PYFMLYALRRVHALRLRAAHGALLRSREATHAHIYADVRVGSHKFDNVIDGGLGDRAE
                     DRESSDWVEAPDDLDPKALQIALWKLTQLKFDEALEDYYDHKKATLSEYLRDEVSAMT
                     RERPIVHKEPLHHDDFPRAAWERVLVDLSRRFLEHPEIYDPCISLSAERIHRWLVTSE
                     GTQVITEDVFIQIGIEGWVLTEDGVYVEGSRQVYLRAIEEVPDRATLERALDEVIAEL
                     ALLREAESPGAYIGPALLSGQAASTLFHEALGHRLEGERLIARGESKTFARKRGERIL
                     PAGLNVLDDPTMTHCGDEPLWGSYRVDDEGVPARRTLLVEDGVLRSFLQSRTPVPGGD
                     QSNGHGRHDGIERPMARMGNFVVEGAPGQGESWDALKARLIELARAQGRRHGVIIRRI
                     LAGETSTADYDFQVFKGTLAEVHLVDATTGASKRIRDVELIGTPLAALQRIVAFGRDQ
                     ETDQGFCYAESGSIPVSGIAPPILLSELELQQSSTTGYHDPLLPPPFADDGSRGRKGG
                     LRSRGKRRKAV"
     gene            3112..>4512
                     /locus_tag="VGB85_06215"
     CDS             3112..>4512
                     /locus_tag="VGB85_06215"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY0133652.1"
                     /translation="MDSPAPIASFAEVMAWLRMQVRITPELGRLQLLTEASGAARSAL
                     LVRDRARLAIVEAGRAPPEEFDVLVLCGPAEVLASALARLPVHRVHALALPTPPPLLQ
                     RAIEEGIRAAQTVLQGRVADQLIDVGLGLNRERDPRRVLELILRHARAITGADAGSVY
                     VVEDDGRRLVFRVAHNDSVAADFTESTLPVSDASIVGACVRTGEIINIPDLSQETAFS
                     HDRSFDARFNYLTRSMLTVPMRAPEGQVVGAVQLINAKHGRMPLRGPEDTAARVHPFS
                     DADARLVQALATQGAVALENVGLYAEIEALFEGFVRASVTAIEQRDPTTSGHSQRVAD
                     LTVALAEAVDRCDLPTFRDYTFTRQELREIEYAGLLHDFGKVGVREQVLTKAKKLHES
                     QLERVLERFEHLRTATRLRWLEAMVASGRSPEDPEMLAALYADFARIDRWRDVVLQAN
                     EPTLLVEQVGQAVHEVA"
BASE COUNT          691 a         1747 c         1472 g          602 t
ORIGIN      
        1 gggcgcgacc gtggcgacga ccgccaccac cacgaccgac ccgaccaccg ccaccaccac
       61 gaccggcgac gacaccaccg gcgcggccga ggccggcacc ggcggtacca ccggcggcac
      121 cacgaccgac cccgacacga cgaccggcga ggacaccacc ggcggcgctg tcagcgagcc
      181 ggcgtgtctg ttcgagccct tcgtcaacaa gggcgcgctg gcgatcgact acgcccagtt
      241 cggcccggtg atcggcagcc actgcaaggg caccaaccac caggacatca ccgacatcga
      301 gcgcgtcgtg ttcctcggcg actcggtgac cgtcggcacg ccgccgacgc cgggcgacgc
      361 ctcctaccgc gccgagctcg ccgacgtgct cgtcgaccgc ttcggcctga ccccgcccaa
      421 tggtctgtgg aagcagtacg acccgttcaa cggcgtctcg gtggtcaagg acgccggcga
      481 cttcagcagc tgttcggagt ggggcgcgcg gaccgacgac ttcctcgccc ccggcaccca
      541 ggtcgacgat tgcttcccgc cggacaagct cgacaagcgc accctggtcg tcttcacgat
      601 gggcggcaac gacctcgccg cgatcgccaa ggacggggcc aacggcaagc cctacgacga
      661 ggtcgtcgcc gacgccgaga aggccctgct gctgatgcgc gaagccgtgc agtggttcgt
      721 cgacgacccg gacaagttcc ccaacggcgt gttcgtcgtg ttcgccaaca tctacgagtt
      781 caccgacggc accggcgacc tcatgtcgtg cccggccgcc ggcctcggcg gcttcgacaa
      841 gccgtgggag gacgtcgagg ccttgaagca actggtcctg tggatcaacg agcaggtgat
      901 ggacatcgcc gtctcgaccc agaccgacat gatcttcatg ctcgagaact tctgcggcca
      961 tggcttccgg gccggcgacc cgaccgcccc gtgctaccgc ggccccgaca gccccaacta
     1021 cttcgacgtg tcctgcatcc acccgacccc ggccggccac gacgtcatca aggacctgtt
     1081 caccgccgtc attgacgagt aatctccact ggcgccggac ccgcaacagc gcggtcctgc
     1141 cagggcactc ctggagacag gttcggttgc gcacgggccg cagggccggt cgtcacgagg
     1201 gacatcgccg cgcctccgct tgccgggccg ccagcccccc cgccggaggc tcccaggcgc
     1261 tgtgcttgcg ccgggatcca cgcgcggcta tgcgtgaggg cccgaggtcg tgatgacagc
     1321 cgtttcgcgc ctgatccccc cctatcaccc cccaggacag cccgaacccc aggagatcct
     1381 ggacgtcatg cagcaggagc tgcggcgttc actggatggc ctcatcgtcc cgggctcacc
     1441 ccggccgtac ttcatgctct acgccctgcg ccgggtccat gccctgcgcc ttcgggccgc
     1501 ccacggcgcc ttgctgcgct cgcgggaagc cacccacgcg cacatctacg cggacgtccg
     1561 ggtcggcagc cacaagttcg acaacgtgat cgacggcggg ctgggcgacc gggccgagga
     1621 ccgcgagagc agcgactggg tcgaggcccc cgacgacctc gaccccaagg ccctccagat
     1681 cgccctgtgg aagctgaccc agctcaagtt cgacgaggcc ctcgaggact actacgacca
     1741 caagaaggcg acgctgagcg agtacctgcg cgacgaggtc agcgcgatga cccgcgagcg
     1801 gccgatcgtc cacaaggagc cgctgcacca cgacgacttc ccgcgcgccg cctgggagcg
     1861 ggtgctcgtc gacctctcgc gccgcttcct cgagcacccc gagatctacg atccgtgtat
     1921 cagcctctcc gctgaacgta ttcatcgctg gttggtcacc tccgagggca cccaggtgat
     1981 caccgaggac gtgttcatcc agatcggcat cgagggctgg gtgctgaccg aggacggcgt
     2041 ctacgtcgag ggctcgcgcc aggtgtacct gcgggcgatc gaggaggtcc cggaccgggc
     2101 gaccctcgag cgcgcgctcg acgaggtcat cgccgagctc gccctgctgc gcgaggccga
     2161 gagccccggc gcctacatcg gccccgcgct gctgtccggc caggccgcca gcaccctgtt
     2221 ccacgaggcg ctcggccatc gcctcgaggg cgagcgcctg atcgcccgcg gcgagagcaa
     2281 gaccttcgcc cgcaagcgcg gcgagcggat cctgcccgcc ggcctcaacg tgctcgacga
     2341 cccgaccatg acccactgcg gcgacgagcc cctgtggggc agctaccgcg tcgacgacga
     2401 gggcgtcccg gctcgccgca ccctcctggt cgaggacggc gtcctgcgca gcttcctgca
     2461 gagtcgcacc cccgtgcccg gcggcgacca gagcaacggc cacggccgcc acgacggcat
     2521 cgagcggccg atggcccgca tgggcaactt cgtggtcgag ggcgcgccgg ggcagggcga
     2581 gtcgtgggac gcgctcaagg cccgcctgat cgagctcgcc agggcccagg ggcgccgcca
     2641 cggcgtcatc atccgcagga tcctcgccgg cgagaccagc accgccgact acgacttcca
     2701 ggtgttcaag ggcaccctcg ccgaggtcca cctcgtcgac gcgaccaccg gcgcgagcaa
     2761 gcggatccgc gacgtcgagc tgatcggcac cccgctggcc gccctgcagc gcatcgtcgc
     2821 cttcggccgc gaccaggaga ccgaccaggg cttctgctac gccgagagcg gatcgatccc
     2881 cgtcagcggc atcgccccgc cgatcctgct gtcggagctc gagctgcagc agagcagcac
     2941 caccggctac cacgacccgc tgctgccgcc gccgttcgcc gacgacggct cgcgcggccg
     3001 caagggcggc ctgcgctcgc gcggcaagcg acgaaaggcc gtctaaggcg cgcgccggcg
     3061 gcccccgcga aagcgcgtgc aagcgcacgc gccggccgct aggctcgcgg ggtggacagt
     3121 cccgccccga tcgccagctt cgccgaggtc atggcgtggc tgcgcatgca ggtccggatc
     3181 acgccggagc tcgggcgcct gcagctcctg accgaggcct ccggcgccgc ccggtcggcc
     3241 ctgctggtcc gcgaccgcgc ccgcctggcg atcgtcgagg ccggccgcgc cccgcccgag
     3301 gagttcgacg tgctggtcct gtgcggcccc gccgaggtcc ttgcctccgc gctcgcgcgt
     3361 ctgcccgtcc accgcgtgca cgccctggcc ctgccgaccc cgcccccgct gctgcagcgc
     3421 gcgatcgagg agggcatccg cgccgcccag accgtcctgc aggggcgcgt cgccgatcaa
     3481 ttgatcgacg tcggcctcgg cctcaaccgc gagcgtgacc cgcggcgggt cctcgagctg
     3541 atcctccgcc acgcgcgggc gatcaccggc gccgacgccg gctcggtcta cgtcgtcgag
     3601 gacgacggcc ggcgcctggt gttccgggtc gcccacaacg actcggtcgc cgccgacttc
     3661 accgagtcga ccctcccggt ctccgacgcc tcgatcgtcg gcgcctgcgt gcgcaccggc
     3721 gagatcatca atatcccgga cctgtcgcag gagacggcgt ttagccacga ccgcagcttc
     3781 gacgcccgct tcaactatct cacgcgctcg atgctgacgg tgccgatgcg cgcccccgag
     3841 ggccaggtcg tcggcgccgt ccagctgatc aacgccaagc acgggcgcat gcccctgcgc
     3901 ggccccgagg acaccgccgc ccgcgtgcac ccgtttagcg acgccgacgc ccgcctggtc
     3961 caggcgctcg ccacccaggg ggccgtcgcg ctcgagaacg tcggcctcta cgccgagatc
     4021 gaggcgttgt ttgaaggctt cgtgcgcgcc agcgtcaccg cgatcgagca gcgcgacccg
     4081 accacctccg gccacagcca gcgcgtcgcc gacctgaccg tcgcgctcgc cgaggcggtc
     4141 gaccgctgcg acctgccgac ctttcgcgac tacaccttta ctcggcagga gctgcgcgag
     4201 atcgagtacg ctggcctgct gcacgacttc ggcaaggtcg gcgtgcgcga gcaggtgctg
     4261 accaaggcca agaagttaca cgagagccag ctagagcgcg tgctcgagcg cttcgagcac
     4321 ctgcgcaccg cgacccgcct gcgctggctc gaggcgatgg tcgccagcgg tcgctcgccc
     4381 gaggacccgg agatgctcgc cgccctgtac gccgacttcg cccgcatcga ccgctggcgc
     4441 gacgtcgtcc tgcaggccaa cgagccgacg ctgttggtcg agcaggtcgg ccaggccgtg
     4501 cacgaggtcg cc
//
LOCUS       DASWNJ010000356         2288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_77896, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000356 DASWNJ010000000
VERSION     DASWNJ010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77896"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1381
                     /locus_tag="VGB85_06220"
     CDS             <1..1381
                     /locus_tag="VGB85_06220"
                     /inference="COORDINATES: protein motif:HMM:NF017545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133653.1"
                     /translation="ESVSVTESASNSESATETASASDTDGDLFCEDVDGDGFGDPAKC
                     TMEMTPGSVPNDDDCDDTSVNTFPGAAENDSMTECQRDEDGDGFGDTSPPPGVDPGTD
                     CSDSDPQANADCGCTPNTAQCDGDDLKTCNAEGTMEAVTTCEFGCDDDGSKCWDALQV
                     DAGPSLCIDPGQEAQLLAMASGGDGMYAYAWTPADTLDDASVADPKATPVAVTEYTVD
                     VSDGEGNMGSDTVSVFVKDQALNLSPEVCTVHNFPHEDNEDPMDPLSNWAWNDETKEL
                     CETLNAKGSALFCGWTLNNATITGTFSVKTIEDDDWVGFMWGIQDTSHFYVFTWKQIG
                     QLATTCGDIQTPAGMQVKVVDVKDPMADPMACVDVHAPADTANSKLLVGVDDFTTNGW
                     LDNTAYTFELTHKTGGEMTIVIRRANNNQLVAQKTFTDTTYASGQFGMYTKSQVNTCF
                     SNFTVDCVQ"
     gene            complement(1385..>2288)
                     /locus_tag="VGB85_06225"
     CDS             complement(1385..>2288)
                     /locus_tag="VGB85_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133654.1"
                     /translation="DFSRAGQICTYEDEVDDCQVGGVAGREYCGFFNDTYEWKWTQCL
                     TETCDTEGAQRDCDGGVQFCAKFIVDDEGAAEQRWGACGEPGECAPGDSEACFPGEGE
                     FEGLDMYCTSDANGRPVWDYEACNTPLVLSFDGALEFTPAPTTAAAFDIHGGTGTCVR
                     ADWPTAATPWLALDRDGDGQIAGGQELFGSATRMPAGTGAHNGFQALAELDSNRDGKI
                     SPTDARWGELVLWADHDADRVSTQWELLPLASFEVVEIDLGYSDRPSCDANGNCGRER
                     AAFVYRTGGAERSGEVIDVRVSCE"
BASE COUNT          397 a          801 c          784 g          306 t
ORIGIN      
        1 cgagtcggtc agcgtgaccg agtccgcgtc caactccgag tcggccaccg agacggcgag
       61 cgcctcggac accgacggcg acctgttctg cgaggacgtc gacggcgacg gcttcggcga
      121 cccggccaag tgcacgatgg agatgacgcc cggctcggtg cccaacgacg acgactgcga
      181 cgacaccagc gtcaacacct tccccggcgc ggccgagaac gactcgatga ccgagtgcca
      241 gcgcgacgag gacggcgacg gcttcggcga caccagcccc ccgcccggcg tcgacccggg
      301 caccgactgc tccgacagcg atccgcaggc gaacgccgat tgcgggtgca cgccgaacac
      361 cgcccagtgc gacggcgacg acctcaagac gtgcaacgcc gagggtacga tggaggccgt
      421 cacgacctgc gagttcggct gcgacgacga cggcagcaag tgctgggacg cgctgcaggt
      481 cgacgccggg ccgagcctgt gcatcgaccc cggccaggag gcccagctgc tggcgatggc
      541 gagcggcggc gacggcatgt acgcctacgc ctggacgccc gcggacacgc tcgacgacgc
      601 cagcgtcgcc gacccgaagg cgacgccggt cgcggtgacc gagtacaccg tcgacgtcag
      661 cgacggcgag ggcaacatgg ggtccgacac ggtcagcgtg ttcgtcaagg atcaggcgct
      721 caacctcagc cccgaggtgt gcacggtcca caacttcccg cacgaggaca acgaggatcc
      781 gatggacccg ctgtcgaact gggcgtggaa cgacgagacc aaggagctgt gcgagacgct
      841 caacgccaag ggctccgcgc tgttttgcgg gtggacgctc aacaacgcga cgatcaccgg
      901 caccttctcg gtcaagacga tcgaggacga cgactgggtc ggcttcatgt gggggatcca
      961 ggacacgtcg cacttctacg tgttcacgtg gaagcagatc ggccagctgg cgaccacctg
     1021 cggcgacatc cagacgcccg cgggcatgca ggtcaaggtc gtcgacgtca aggacccgat
     1081 ggccgacccg atggcctgcg tcgacgtcca cgcgccggcc gacaccgcca actcgaagct
     1141 gctggtcggc gtcgacgact tcaccaccaa cggctggctc gacaacaccg cgtacacctt
     1201 cgagctgacc cacaagaccg gcggcgagat gacgatcgtg atccggcgcg ccaacaacaa
     1261 ccagctggtc gcgcagaaga ccttcaccga cacgacctac gccagcgggc agtttggcat
     1321 gtacaccaag tcgcaggtca acacctgctt ctcgaacttc acggtcgact gcgtgcagtg
     1381 agcgctactc gcaggacacg cgcacgtcga tgacctcgcc gctgcgctcc gcgccgcccg
     1441 tgcgatacac gaaggcggcg cgctcgcgac cgcagttgcc gttcgcgtcg caggacgggc
     1501 ggtcgctgta gccgaggtcg atctcgacga cctcgaagct ggcgagcggc agcagctccc
     1561 actgggtcga gacgcggtcg gcgtcgtggt cggcccacag caccagctcg ccccagcgcg
     1621 cgtcggtcgg gctgatcttg ccgtcgcggt tggagtcgag ctcggcgagc gcctggaagc
     1681 cgttgtgcgc gccggtgccg gcgggcatgc gggtcgccga gccgaacagc tcctggccgc
     1741 cggcgatctg gccgtcgccg tcgcggtcga gcgcgagcca cggggtcgcg gcggtcggcc
     1801 agtcggcgcg cacgcaggtg ccggtgccgc cgtggatgtc gaacgcggcc gcggtcgtcg
     1861 gcgccggggt gaactcgagc gcgccgtcga agctgagcac gagcggggtg ttgcacgcct
     1921 cgtagtccca cacggggcgg ccgttggcgt cgctggtgca gtacatgtcg agcccctcga
     1981 actccccctc gccggggaaa caggcctcgg agtcgccggg ggcgcactca ccgggctcac
     2041 cgcaggcgcc ccagcgctgc tcggccgcgc cttcgtcatc gacgatgaac ttggcgcaga
     2101 actggacgcc gccgtcgcag tcacgctgcg cgccctcggt atcgcaggtc tcggtgaggc
     2161 actgcgtcca cttccactcg taggtgtcgt tgaagaagcc gcagtactcg cggccagcga
     2221 cgccgccgac ctgacagtcg tcgacctcat cctcgtaggt gcagatctgg ccggcgcgcg
     2281 agaagtcg
//
LOCUS       DASWNJ010000357         5043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_7079, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000357 DASWNJ010000000
VERSION     DASWNJ010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7079"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VGB85_06230"
     CDS             <1..577
                     /locus_tag="VGB85_06230"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY0133655.1"
                     /translation="LGQLQSSLDKIWDVKASKRAWFAVLRSRFMSLLFILLLGTAVIA
                     SLIASSIITALSNHTVDALDWMWRVADIGVPLVLMTGLFAMLYKMLPDAEIRWRDVAI
                     GAIITSVLFTLGRFLIGIYIGRAGVTSAYGAAGSVIGLMVWVFYSSVIVLFGAEITQV
                     YARRYGGDIRQKRFIGEAPDAVNPPAAPQKR"
     gene            879..1592
                     /locus_tag="VGB85_06235"
     CDS             879..1592
                     /locus_tag="VGB85_06235"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEY0133656.1"
                     /translation="MHVTKSILLALSIGTLLTGGCKKDSSDTAPPEPAVTTPPPADPA
                     PADPAPTDAAAGDAGGDAAATTPAAPPPPPPLADAEIAAVAKAANDGEIETSKLAKTM
                     AKDKKVKDFAAMMVKDHTAANKRQASLIKKAKIEAKENEMSTRLTTDAKSRVDTLKGL
                     EKGAAFERAYIDAQVEMHTTVLTAIDTQMLPGAQNPDLKAELTTMRTAVEGHLNMAKE
                     IQTGLAAAPAGDAAAKPAS"
     gene            complement(1702..2790)
                     /locus_tag="VGB85_06240"
     CDS             complement(1702..2790)
                     /locus_tag="VGB85_06240"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEY0133657.1"
                     /translation="MRAAVIAVLLTGACAGRYPARPAAAGSAVAGLPEVPRRCLVANH
                     VHTIVSDRYSHAPGDANRPHAYSPQGLRTAIAEFTALGADAIVVTDHNAIDAIFDPQV
                     AAARLEVIGGMEWTTRRGHALILGAAATSPADAILPPSWRARIGPADFRSMVDTTHAR
                     GGVVIIAHPRVPFRTWPDDTFGADGVEVWGLDNALMRNAASLRWWHERLVDGLKLVAI
                     GGTDLHPGAGVRRHHHPLNWVYAPTCDEPAILAAIRAGHLLVVRGRDTPVVVLGAETG
                     GAVDFADAREGDTLPAGDIDLQVRVVGGAKARLRLLGRAGVLLDRTVDAADESLRLRL
                     AVRPGDFVRAELRRGRRLLAVGNPLYVR"
     gene            2830..3318
                     /locus_tag="VGB85_06245"
     CDS             2830..3318
                     /locus_tag="VGB85_06245"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0133658.1"
                     /translation="MSIVLRPATPEDAPEIHRLIVALATYEREPDAVVVTPTELAAQL
                     ASERPPFECLLAEVDGAVVGFALYFHNYSTWRGRAGLYLEDLFVEPSQRGRGVGRRLL
                     GALAKIAVDRGCARMEWMVLDWNEPAIAFYRSLGARPVDGWHVFRLTDAALADLAARP
                     AR"
     gene            complement(3320..3661)
                     /locus_tag="VGB85_06250"
     CDS             complement(3320..3661)
                     /locus_tag="VGB85_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133659.1"
                     /translation="MQRPATTRRRARPRGLQVLQVLRVWCAPVGALAAALLSFWTGGV
                     HLAALAVLAALYLVSVLRGGRARDPLGLLAGFLAPAVAVGVLTLSPLGALEGLLWGGF
                     VRLFLLEQARA"
     gene            complement(3729..4559)
                     /locus_tag="VGB85_06255"
     CDS             complement(3729..4559)
                     /locus_tag="VGB85_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133660.1"
                     /translation="MVQRCVLTSILAASLALAPPPARATDPPVRPDLTSLDEVILKNG
                     TLVRGTIVEYIPGTSVTISSAADGKLLRFPADEVAATTLASRVPEPPELFGQDDAPLL
                     HVEVSGSAQVQLHELLEPEDIRWTGLEWGRQVERVRYRIRCRAPCELAIDARHGRRFF
                     FASDEVPRSRTFSLAGMGPDVRAHVRPGSSQRMIGGMIAAPIGVAGVVLGTIFAATSD
                     ADDSRMTTPGSAIAGLGAMLIATGIVLLVTGRTRVRLVPGAARPRVTKLPSYTPAAFH
                     "
     gene            complement(4623..>5043)
                     /locus_tag="VGB85_06260"
     CDS             complement(4623..>5043)
                     /locus_tag="VGB85_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133661.1"
                     /translation="AELPPLVLRHNLPNSTGGKLVGAAFSSLRDHFVDLLLSSEKSYR
                     GTLLGMRHGVDLVELIQYVSRQEGDLALSQWCATWLEKRRPLVEAAAGELAWFAANPD
                     RAREAAKDNPLALGLQALVNGFEQIAEKVRQAIPKQT"
BASE COUNT          738 a         1748 c         1863 g          694 t
ORIGIN      
        1 gctgggtcag ctgcagtcct cgctcgacaa gatctgggac gtcaaggcgt cgaagcgggc
       61 gtggttcgcg gtgctgcggt cgcgcttcat gagcctgttg ttcatcctgc tgctcggcac
      121 cgccgtgatc gcgtcgctga tcgccagctc gatcatcacg gcgctgtcca accataccgt
      181 cgacgcgctc gactggatgt ggcgcgtcgc ggacatcggc gtgccgctgg tgctgatgac
      241 cggcctgttc gcgatgctct acaagatgct gccggacgcc gagatccgct ggcgagacgt
      301 cgcgatcggg gcgatcatca cctcggtcct gttcacgctc ggccggttcc tgatcggcat
      361 ctacatcggt cgcgccggcg tgacctcggc gtacggcgcc gcgggctcgg tgatcggcct
      421 gatggtgtgg gtgttctact cgtcggtcat cgtgctgttc ggcgccgaga tcacgcaggt
      481 ctacgcgcgt cgctacggcg gcgacatccg ccagaagcgc ttcatcggcg aggcaccgga
      541 cgcggtcaac cctccggccg cgccgcaaaa gcgctgaaac cctggctctc ccgggtcggt
      601 ctcactggtt cctgacggag ccgggggccg acccgtttta ttttggggtg caaagccccc
      661 gctctgctgt ccagaggccg caacgtggaa cttgcgcact cctgcgcgac acccgctgtg
      721 accgtgatgc ccggagatgc cccctgtgtg cacggtcgcc tgtcactcgc gcccgaacgc
      781 gcaggagggc gcgtagaccc ggttttgcgg cgttccaggg cccgcggcag ggccgcctgg
      841 cgtggcgcga gggcacgatc gcggtcacga agggcgccat gcacgtgacc aagtccattt
      901 tgctcgccct ctcgatcggc accctgctca ccgggggctg caagaaagat agttcggaca
      961 ccgccccgcc cgagccggcc gtgacgaccc cgccgcccgc cgacccggcg cccgccgatc
     1021 cggcgcccac ggacgctgcc gcgggtgacg ctggtggcga cgccgccgcg accacgccgg
     1081 ccgctccgcc cccgccgccc ccgctcgccg acgccgagat cgccgcggtg gccaaggccg
     1141 ccaacgacgg tgagatcgag acctccaagc tcgccaagac catggccaag gacaagaagg
     1201 tcaaggactt cgccgcgatg atggtcaagg accacaccgc cgccaacaag cgtcaggcgt
     1261 ccctgatcaa gaaggccaag atcgaggcca aggagaacga gatgagcacc cgtctgacca
     1321 cggatgccaa gtcccgcgtc gacaccctca agggcctcga gaagggcgcc gcgttcgaga
     1381 gggcgtacat cgatgcccag gtcgagatgc acaccaccgt gctgaccgcg atcgacaccc
     1441 agatgctgcc cggcgcgcag aacccggacc tcaaggccga gctgaccacg atgcgcaccg
     1501 ccgtcgaggg tcacctcaac atggccaagg agatccagac cggtctcgcc gccgcccccg
     1561 cgggcgacgc tgctgccaag cccgccagct gagcgacttt ctttcgcatt ctttcgcatt
     1621 ctttcgcatt cttccgcatt cgtggaagcg aacgacgccg gcggggccac ctgccggcgt
     1681 cggcgtttaa tcgggccgtt tttagcggac gtacagcggg ttgccgacgg cgagcaggcg
     1741 acggccccgg cgtagctcgg cgcgcacgaa gtcgccgggg cggacggcca ggcgcaggcg
     1801 caggctctcg tcggcggcgt cgaccgtgcg atcgagcaat acgcccgcgc ggccgagcag
     1861 gcgcagccgg gcctttgcgc cgccgaccac gcggacctgc aggtcgatgt cgccggcggg
     1921 cagggtgtca ccctcgcggg cgtcggcgaa gtcgacggcg ccgcccgtct cggcgccgag
     1981 cacgacgacg ggcgtgtcac gcccgcggac gacgagcagg tggccggccc ggatcgccgc
     2041 gaggatcgcc ggctcgtcgc aggtcggcgc gtacacccag ttgagcgggt gatggtggcg
     2101 gcggaccccc gcgccggggt ggaggtcggt gccgccgatc gctaccagct tcaggccgtc
     2161 gacgaggcgc tcgtgccacc agcgcagcga ggcggcgttg cgcatcaagg cgttgtcgag
     2221 gccccacacc tcgacgccgt cggcgccgaa ggtgtcgtcc ggccaggtcc ggaacgggac
     2281 ccgcgggtgg gcgatgatca ccaccccgcc gcgtgcgtgg gtggtgtcga ccatcgaccg
     2341 gaagtcggcg gggccgatcc gggcgcgcca cgacggcggc aagatcgcgt cggccgggct
     2401 cgtcgccgca gcgccgagga tcagcgcgtg gccgcggcgg gtcgtccact ccatgccgcc
     2461 gatcacctcg agccgggccg ccgcgacctg cgggtcgaag atcgcgtcga tcgcgttgtg
     2521 gtcggtgacg acgatcgcgt cggcgccgag cgccgtgaac tcggcgatcg ccgtgcgcag
     2581 gccctgcggg ctgtaggcgt gggggcggtt ggcgtcgccc ggggcgtggc tgtagcgatc
     2641 cgagacgatc gtgtgcacgt gattggcgac caggcagcgg cgcgggacct ccggtagacc
     2701 ggcgaccgcc gacccggccg cggcggggcg cgcggggtag cgaccggcgc aggcaccggt
     2761 gagcagcacg gcgatcacgg cggcacgcac cccgcgaagt taacacgcgt cctgtgccac
     2821 agtcccgcca tgtcgatcgt gctgcgaccc gcgacccccg aggacgcccc ggagatccac
     2881 cgcctgatcg tggcgctcgc gacctacgag cgcgagccgg acgcggtggt cgtcacgccg
     2941 acggagctgg cggcgcagct ggcgagcgag cgcccgccct tcgagtgcct gctcgccgag
     3001 gtcgacggcg cggtcgtcgg cttcgccctg tattttcaca attactcgac ctggcgcggg
     3061 cgagccgggc tgtacctcga ggacctgttc gtcgagccgt cgcagcgcgg gcgcggcgtc
     3121 ggccggcgct tgctcggcgc gctggcgaag atcgccgtcg accgcggctg cgcgcgcatg
     3181 gagtggatgg tgctcgactg gaacgagccg gcgatcgcgt tctaccgctc gctcggcgcc
     3241 cgcccggtcg acggctggca cgtgttccgc ctgacggacg cggcgctcgc cgacctggca
     3301 gcacgacccg ctcgatgact caggcccgcg cctgctcgag caggaacagg cgcacaaagc
     3361 cgccccacag caggccctcg agcgcgccga gcgggctcag ggtcaacacg ccgacggcga
     3421 ccgcaggggc gaggaacccc gcgagcaggc cgagcgggtc gcgggcccgg ccgccgcgca
     3481 ggaccgacac caggtacagc gcggcgagca cggcgagcgc ggcgaggtgc acaccacctg
     3541 tccaaaagga cagcagcgcg gcggcgagcg ccccgaccgg cgcgcaccag acccgcagga
     3601 cctgcaggac ctgcaggccg cgagggcggg cgcggcggcg ggtggtggcg ggacgttgca
     3661 tcgagggcgc cgtgtccgtc tgcgacagcg tcggacatgt cgatagcgac gcgtgtcggt
     3721 gcggcggatc agtgaaaagc ggccggcgtg tacgatggta gcttcgtgac gcggggtctg
     3781 gcggccccgg gaacaagtcg cacgcgcgtg cgcccggtga cgagcaggac gatgccggtg
     3841 gcgatcagca tcgcgccgag ccccgcgatc gcgctgccgg gcgtggtcat ccggctgtcg
     3901 tcggcgtcgc tggtcgcggc gaagatggtc ccgagcacga cgccggcgac gccgatcggc
     3961 gcggcgatca tgccgccgat cattcgctgg ctgctgcccg ggcgcacgtg ggcgcggacg
     4021 tcggggccca tgccggcgag gctgaaggtc cgcgagcgcg ggacctcgtc gctggcgaag
     4081 aagaagcggc ggccgtggcg ggcgtcgatc gcgagctcgc agggcgcgcg gcagcggatc
     4141 cggtagcgca cgcgctcgac ctgtcggccc cattcgagcc cggtccagcg gatgtcctcg
     4201 ggctcgagca gctcgtgcag ctgcacctgg gccgagccgc tgacctcgac gtgcagcagc
     4261 ggcgcgtcgt cctggccgaa cagctcgggc ggctccggca cgcgcgacgc gagggtcgtc
     4321 gcggcgacct cgtcggccgg gaagcgcagg agcttgccgt cggccgcgga cgagatcgtc
     4381 accgaggtgc cggggatgta ctcgacgatc gtgccgcgca cgagcgtgcc attttttaag
     4441 atgacctcgt cgagggatgt aaggtcaggg cgcaccggcg ggtcggtggc gcgggccggt
     4501 ggtggcgcga gggcgaggct cgcggcgagg atcgatgtca ggacgcaacg ctggaccatg
     4561 ataagttatc ggtccagcgt agcggtcgcg gggctcgcgg tccaatgccc gcggcctggc
     4621 gctcaggtct gcttcgggat cgcctggcgg accttttcgg cgatctgctc gaagccgttg
     4681 acgagcgcct ggaggccgag cgcgagcggg ttgtccttgg cggcctcgcg ggcgcggtcc
     4741 gggttggcgg cgaaccacgc gagctccccg gcggcggcct cgacgagcgg gcggcgcttc
     4801 tcgagccagg tcgcgcacca ctgcgacagc gccaggtcac cctcctgacg cgagacgtac
     4861 tggatcagct cgacgaggtc gacgccgtga cgcatgccga gcaaggtccc gcggtacgac
     4921 ttctcggagc tgagcagcag gtcgacgaag tggtcgcgca gcgacgagaa cgcggcgccg
     4981 acgagcttgc cgccggtgct gttggggagg ttgtgccgaa gcaccagcgg cggcagctcg
     5041 gcg
//
LOCUS       DASWNJ010000358         1550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_77941, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000358 DASWNJ010000000
VERSION     DASWNJ010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77941"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VGB85_06265"
     CDS             complement(<1..445)
                     /locus_tag="VGB85_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0133662.1"
                     /translation="MFARSNPKASAKLALALATALTTGACAASLAPLPAPDPAPTASA
                     ERVEHRHEVLFAIDQAEASPGELVRLDAFLAQLPTGSIRRFRLIAHADEPAGETYNLA
                     LSARRARAVERHLRARLPARDEIATTALGERSSSRLAETPGPVPRD"
     gene            complement(454..>1550)
                     /locus_tag="VGB85_06270"
     CDS             complement(454..>1550)
                     /locus_tag="VGB85_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HEY0133663.1"
                     /translation="HLSGLRRLLSEIAPDSAIEVKSVEGGIVLDGAVANPVQAQEARE
                     AAGRFLGDKETLINRLEVGAPTQVNLRVRVVEVAREVTKLLGVNLEGLLDPGNFVFGL
                     ATGRAFTTAGGAPFSRFADASGQSGSLLGSYSTDNLTVNGIVDALEREGLVNLLAEPN
                     LTAISGETASFLAGGEFPVPVGRDENEISIEFKQFGVSLAFTPTVLGADRISLRVRPE
                     VSDLADKGSIKLADLVIPALSTRRAETTVELGSGQSFAIGGLISNTTRNSVEKFPGLG
                     DLPILGALFRSTNFRRSESELVIIVTPYLVRPVSTARLASPLDGRREAGDLERILTGR
                     LARAATRPGAPPLPDASAERLAGSAGFALD"
BASE COUNT          229 a          493 c          589 g          239 t
ORIGIN      
        1 tgtcacgggg aaccgggccg ggcgtttcgg cgagcctgga tgaggaccgc tcacccaagg
       61 cggtcgtcgc gatctcgtcg cgtgccggga gccgcgcccg caggtggcgt tcgaccgcgc
      121 gcgctcggcg tgcagacaaa gcgagattgt aggtctcgcc ggcaggctcg tcggcgtggg
      181 cgataaggcg gaagcgccgg atcgatcctg taggtagctg ggccaaaaag gcgtcgagcc
      241 ggaccagctc gccagggctg gcctccgcct ggtctatcgc gaagagcacc tcgtgccggt
      301 gctcgacgcg ctcggccgag gcggtcggcg ccgggtccgg tgccggaagc ggcgcgaggc
      361 tcgcggcgca ggcgccggtg gtgagcgcgg tcgcgagggc gagcgcgagc ttagccgacg
      421 ccttgggatt gctgcgggcg aacatgggtt acctcagtcc agcgcgaagc cggccgatcc
      481 ggcgagccgt tctgccgagg cgtcgggaag cggtggcgca cccgggcgtg tggcggcgcg
      541 ggcgaggcgt cctgtcagga tacgctcgag gtcgccggct tcccgtcggc cgtcgagcgg
      601 gctcgcgagg cgggccgtcg agaccggccg taccaagtat ggtgtgacga tgatgaccag
      661 ctccgactcg ctgcggcgaa agttggtaga gcggaagagc gcgccgagga tcggcaggtc
      721 gccgaggccc gggaacttct cgacgctgtt gcgggtcgtg ttcgagatta ggccgccgat
      781 cgcgaagctc tgaccgctgc cgagttcgac ggtggtctcc gcgcggcggg tcgagagcgc
      841 cgggatgacg agatcggcga gcttgatgga tcccttgtct gccaggtcgc tcacttccgg
      901 gcggacccgt aggctgatcc ggtcggcgcc tagcacggtc ggcgtgaatg cgaggctgac
      961 gccgaactgc ttgaactcga tcgagatctc gttctcatcg cggccgaccg ggaccgggaa
     1021 ttcgccgccg gccaggaagc tcgcggtctc gcccgagatc gcggtcaggt tcggttcggc
     1081 cagcaggttg acgaggcctt cgcgctccag ggcgtcgacg atgccgttga ccgtcaggtt
     1141 gtcggtcgag tagctgccga gcagcgatcc ggactgtccg ctcgcgtcgg cgaatcggga
     1201 gaagggggcg ccgccggcgg tggtgaacgc gcggccggtg gcgaggccga aaacgaaatt
     1261 gccggggtcc aggaggcctt ccaggttgac gcccagcagc ttcgtgacct cgcgggcgac
     1321 ctccaccacg cggacgcgca ggttcacctg agtcggggcg ccgacctcca ggcggttgat
     1381 caacgtctcc ttgtcgccca ggaaacggcc ggcggcctca cgggcctcct gcgcctggac
     1441 cggattcgcg accgcgccgt cgaggacgat cccgccctcc acggacttga cctcgatcgc
     1501 actgtcggga gcgatctccg acaatagtcg ccgcaacccc gagaggtggt
//
LOCUS       DASWNJ010000359         7163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_112791, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000359 DASWNJ010000000
VERSION     DASWNJ010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7163
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112791"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="VGB85_06275"
     CDS             <1..1053
                     /locus_tag="VGB85_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133664.1"
                     /translation="ALFLILATAALLTTARPAAACETDSPDPGLSFELDGFDAHPVAE
                     GGALLLNAQAYGIDIDAALATLVLWEVRRDGVVQPGSFEVVPLWDAAATSVSPENHKF
                     ALIWRPDAPFTADGVYTVDFEIAGYWQTGPQQAPLRVVPAEPPALPYLLWAPSTEAVD
                     PSEVICCETGLNSCGTSTQCEATRATLLPALYLELELDAGHESQILWVARASPGGEIG
                     PRVQGRGEYVVGTWRWTGTVRVDAEQYCVVVGVTSVTDGSEVVSEPLCHDQAKLGAHT
                     AEIPPSARMSLYDPANPGDGRCIGPIVYEADGAAYPRGSEEPEASGGCDMTAGGGSWL
                     VLLVLLALIRRRRARA"
     gene            complement(1025..2629)
                     /locus_tag="VGB85_06280"
     CDS             complement(1025..2629)
                     /locus_tag="VGB85_06280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0133665.1"
                     /translation="MDTWDLIVIGGGPAGATVAAIAARGGMRVLLLEADVHPRVHVGE
                     SLLPGIMPILDRMGALAAVEAEGFTPKTGTTHWQWGATPRWDLWFSDTDAYDHAYLVD
                     RARFDAILFAAAGDAGAVTHTHAAARAFLWDGERCTGVRWRRRGANEEQTSHGFVIDA
                     SGQAALLARQLGLRETIAGLQHQASWAHYEHAGRLPPPREHQALFVAERDHWLWLFPL
                     SPTRASVGMVRLDPGTTSEDDDTRAARFEAAVAGSPLFSGVLGAAERVTPVRTQRDWS
                     YRMRSVAGPGWLLVGDAAGFIDPVLSTGVFLAMHAGFHAAELLLARHRGELDEAILIR
                     RYQQHHVELFGDLLRMVRFYYQQTLTRDDYFWESKKILLREDTELRPQKAFLILTSGL
                     VRNLAYEAVSDAADARRAASLSGAEIGEHDPEALGFICFHVTFAGHGEPAALYLLIEP
                     RRPAEPTLYRTRSWHINCLAPRFNNDPIREPTLAGPLRAVAEAVRALDDRDEPLAAFW
                     RRNRSALITALTTMGPGFTLERVFGE"
     gene            2817..4487
                     /locus_tag="VGB85_06285"
     CDS             2817..4487
                     /locus_tag="VGB85_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0133666.1"
                     /translation="MRTATRTANKVFALTFCSLLGACLDHPVEEVRYDKTVVPTTMLP
                     IDLNRDVDILFVIDNSGSMAEEQARLARNFPTFIASLDDMGADYRIGITTTDVKHPGC
                     TTDASPEDGALVLRSCTEAVADGAFQFNDLDAAYACTDQCKLGPDQLRVTASADGEVH
                     PWLESISGVQNLPEGVAMADAFACFGPQGINGCGYESPLEAMRLAISKARADGGTGFM
                     RDEALLSVVLVTDEADCSSNPAHADIFTTNPTFQNDTDPKRTSAICWRAGVACTGEGP
                     EFKECHAEDYSADGAPGVTDADDAVLHPIDRYVEFLEGLRAGGDAPLADGGPLAREVL
                     VSLISGVPVGYDQGTAEIEYSAAEPGSPQDINFGVAPGCVNTEDGSNSTAIPPVRERE
                     VAEAFAGSDGARNVFSICQDDYSPALEQIAAAVRRALPPACAGACIEDSNPETKVLEP
                     VCSVTANFADGGSEQVPACVVDGDEWAVPKGASVCFAMLVDPDGRTPFTGDDMTRVTT
                     DGGAPAPACSTDTENVEFKLLRAGPRRAGVSYEAACVAADDNTTCAAD"
     gene            4550..5971
                     /gene="nadA"
                     /locus_tag="VGB85_06290"
     CDS             4550..5971
                     /gene="nadA"
                     /locus_tag="VGB85_06290"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF014498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEY0133667.1"
                     /translation="MSQFPSLLLRHGGVSAQGAFAEAQAAFLDPDPTTVQRLRSLLAA
                     SEAGVVAHFYMDAELQGALLATKWQRLHISDSLVMADRAVAMAQQGARAVVVLGVDFM
                     SENVRAVMDSAGFGHVPVYRVTAEAIGCSLAAAAEAPAYAAYLRRAAQTPNSLHVVYI
                     NTSLRTKANAQAILPTITCTSSNVIQTILETFASDPSANVWFGPDTYMGHNLRVLFER
                     LAEMDDAAIAALHPKHDRASVAALLPRFHYFEQGNCIVHHMFGAEVVARVRDAYPDAH
                     ITAHLEVPGEMFALALAAQGHGRGVVGSTSDILGYITKQVKAAPAGPGHLQFVLGTEA
                     GMVTAIVHAVREQLAARPELTVEIVFPVAEGAVLATGDAELPLVPGVAGGEGCSTAGG
                     CATCPYMKMNSLDALMAVLERLQTPAALSAHAPRVYREQIGGQTVAQLGELPIRHMRD
                     FQKSGHLPPALVEDMRTRAAAAG"
     gene            complement(5990..>7163)
                     /locus_tag="VGB85_06295"
     CDS             complement(5990..>7163)
                     /locus_tag="VGB85_06295"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY0133668.1"
                     /translation="VTIGECPGGMGQCALDPNGAPLVVTPPFKIQIPLNSYSLSASIA
                     VPISDYILSLAPARKGTHAARESVILARDAERIKIQTDARLAYFNWLRGVASLIVIED
                     ALQRTQSRLADVQNLFDAGSATRSDVLRVDALVSSQQAAIADARAYRKIAERSLSIMM
                     KEPPKDFVVGEDLLAAPADLGPLPSVDELIAEAHQNRLELRSLEWAIDGTHQGIRATR
                     AGYYPRLDAFADALYANPNQRFFPLAPVWRGSWSVGVQLSYTINQTLRTKVEVRQLKA
                     DKRELLLQAEMMRRGIAMEVAQAYHDRERALAAIELSTRGLASAAEAYRVASENYQAG
                     AATTNEIIDAEGDQVQAGLRAVNSRIDLRVATARVLYATGRLNGDVKPKQVDRGAR"
BASE COUNT         1046 a         2588 c         2527 g         1002 t
ORIGIN      
        1 gcgctgtttt tgatcctggc gaccgcggcg ctgctgacga cggcgcggcc cgccgctgcg
       61 tgcgagaccg actcgcccga cccgggcctg tcgttcgagc tcgacgggtt cgacgcgcat
      121 ccggtcgcgg agggcggcgc gttgctgctc aacgcgcagg cgtatggcat cgacatcgac
      181 gccgcgctcg cgacgctggt gctgtgggag gtgcgccgcg acggtgtggt gcagcccggt
      241 agcttcgagg tcgtgccgct gtgggacgcc gcggccacca gcgtgtcgcc ggagaatcac
      301 aaatttgcgc tgatctggcg gcccgacgcg cccttcaccg cggacggcgt atacaccgtc
      361 gacttcgaga tagcaggcta ctggcagacc gggccgcaac aggcgccgct gcgtgtcgtc
      421 cccgcggagc cgcccgcgtt gccctacctg ctctgggcgc cgtccaccga ggcggtcgac
      481 ccatccgaag tcatctgctg cgagaccggc ctgaattctt gcggcacgag cacccaatgc
      541 gaggcgacgc gggcgaccct gctgccggcg ctctacctcg agctcgagct cgacgccggc
      601 cacgagagcc agatcctgtg ggtcgcgcgg gcgtcgcctg gcggggagat cgggccgcgc
      661 gtgcagggac gaggggagta cgtcgtcggg acctggcggt ggaccggtac cgtccgggtc
      721 gacgccgagc aatactgcgt cgtcgtcggc gtgacctcgg tgaccgacgg cagcgaggtc
      781 gtatcggagc cgctgtgcca cgaccaggcg aagctcggcg cacacacggc ggagatccca
      841 ccgagcgcca ggatgagcct gtacgacccc gccaaccccg gagacgggcg ctgcatcgga
      901 ccgatcgtgt acgaggccga cggcgcggcc tatccccgcg gcagcgagga gccggaggcg
      961 tcggggggtt gcgacatgac ggccggcggc ggctcgtggc tcgtgctgct cgtgctgctc
     1021 gcgctcattc gccgaagacg cgctcgagcg tgaagcccgg gcccatcgtg gtcagggcgg
     1081 tgatcaacgc actacgattg cgccgccaga acgccgcgag cggctcgtcg cggtcgtcga
     1141 gcgcacgcac ggcctcggcc acggcgcgca gcggcccggc gagcgtgggc tcgcggatcg
     1201 ggtcattatt gaaacgcggg gccaggcagt tgatgtgcca gctgcgcgtg cgataaagcg
     1261 tcggctcggc cggacgccgg ggttcgatca acaggtacag cgcggccggc tcgccgtgtc
     1321 ccgcgaaggt gacgtggaag cagatgaagc cgagcgcctc ggggtcgtgc tcgccgatct
     1381 cggcgccgga gaggctggcc gcgcggcggg cgtcggcggc gtcgctgacg gcctcgtagg
     1441 cgaggttgcg gaccaaacca ctggtcagga tcaagaacgc cttctgcggg cgaagctccg
     1501 tgtcctcgcg cagcaggatc ttcttcgact cccagaagta gtcgtcgcgc gtcagggtct
     1561 gttgataata aaaccggacc atccgcagca ggtcgccgaa cagctcgacg tggtgctgct
     1621 gatagcgtct tattaaaatt gcctcgtcga gctcgccgcg gtggcgcgcg agcagcagct
     1681 cggccgcgtg aaagccggcg tgcatcgcca ggaacacgcc ggtcgacagc acagggtcga
     1741 tgaagcccgc cgcgtcgccg acgagcagcc agccgggacc ggcgacgctg cgcatgcggt
     1801 agctccagtc gcgctgggtt cgcaccggcg tcacgcgctc ggccgcgccg agcaccccgg
     1861 agaacagcgg gctgccggcg accgcggcct cgaagcgagc ggcccgggtg tcgtcgtcct
     1921 cgctggtggt gccggggtcg aggcggacca tgccgaccga ggcgcgcgtc ggcgacagcg
     1981 ggaacagcca cagccagtgg tcgcgctcgg cgacgaacag cgcctgatgt tctcttggcg
     2041 gcggcaggcg gcccgcgtgc tcgtagtggg cccacgaggc ctggtgttgc aggccggcga
     2101 tcgtctcgcg caggccgagc tggcgggcga gcagcgcggc ctgacccgac gcgtcgatca
     2161 cgaagccgtg ggaggtctgc tcctcgttcg ccccacgtcg tcgccagcgc acgccggtgc
     2221 agcgctcgcc gtcccacagg aacgcgcggg ccgccgcgtg cgtgtgggtc acggcgcccg
     2281 cgtccccggc agcggcgaac aggatcgcgt cgaagcgggc gcggtcgacc aggtaggcgt
     2341 ggtcataggc gtcggtgtcg gagaaccaca ggtcccagcg cggcgtggcg ccccactgcc
     2401 agtgcgtcgt gccggtcttc ggcgtgaagc cctcggcctc gaccgccgcg agcgcgccca
     2461 tgcgatcgag gatcggcatg atgccgggca gcagactctc gccgacgtgt acgcgcgggt
     2521 ggacgtcggc ctcgagcagc agcacacgca tgccgccgcg cgccgcgatc gcggccaccg
     2581 tcgcgccggc cggccccccg ccgatgacga tcaagtccca ggtgtccacg accgcgcgag
     2641 gctacggttc gcctcgccgg ggtgtcaacc tggttgtcta ctgccaccgc cggggccacc
     2701 gaagttgtcg cctggagacc gcgaggcaac gcacaagatc aataaaatcc aataaagtcg
     2761 tggtggtacg ccgcctgcaa tccctgtcct cgtccccgaa gaccggagtg cctcttatgc
     2821 gtaccgccac cagaaccgcc aacaaagttt tcgccctgac cttttgcagc ctgctcggcg
     2881 cctgcctcga ccacccggtc gaggaggtcc gctacgacaa gaccgtggtg ccgacaacga
     2941 tgttgccgat cgacctcaac cgcgacgtcg acatcctgtt cgtcatcgac aactcgggct
     3001 cgatggcgga ggagcaggcc cggctcgcgc gcaacttccc gaccttcatc gccagcctcg
     3061 acgacatggg cgcggactac cggatcggca tcacgaccac cgacgtcaag caccccggct
     3121 gcaccaccga cgccagcccc gaggacggcg cgctggtcct gcgcagctgc accgaggcgg
     3181 tcgccgacgg cgcgttccag ttcaacgacc tcgacgccgc ctacgcctgc accgaccagt
     3241 gcaagctcgg ccccgaccag ctgcgcgtca cggcctcggc cgacggcgag gtccacccct
     3301 ggctcgagtc gatctccggc gtgcagaacc tgcccgaggg cgtggcgatg gcggacgcct
     3361 tcgcctgctt cggcccgcag ggcatcaatg gttgcggcta cgagtcgccg ctcgaggcga
     3421 tgcgcctggc gatctcgaag gcgcgcgccg acgggggcac cggcttcatg cgcgacgagg
     3481 cgctgctctc ggtcgtgctc gtcaccgacg aggccgactg cagcagcaac cccgcgcacg
     3541 cggacatctt caccaccaac ccgaccttcc agaacgacac cgaccccaag cgcaccagcg
     3601 cgatctgctg gcgcgccggc gtcgcctgca ccggcgaggg gcccgagttc aaggagtgcc
     3661 acgccgagga ttacagcgcc gacggtgcgc ccggcgtcac cgacgcagac gacgcggtgc
     3721 tgcacccgat cgaccgctac gtcgagttcc tcgaaggtct gcgcgccggc ggcgacgccc
     3781 cgctcgccga cggcggcccg ctcgcccgcg aggtgctcgt ctcgctgatc tccggcgtac
     3841 cggtcggcta cgaccagggc accgccgaga tcgagtacag cgccgccgag cccggctcgc
     3901 cgcaggacat caacttcggc gtcgcgcccg gctgcgtcaa caccgaggac ggcagcaaca
     3961 gcaccgcgat cccgccggtg cgcgagcgtg aggtcgccga ggcgttcgcc ggcagcgacg
     4021 gcgcgcgcaa cgtgttctcg atctgccagg acgactacag cccggcgctc gagcagatcg
     4081 ccgccgccgt gcgccgcgcg ctgccccccg cgtgcgcggg cgcctgcatc gaggacagca
     4141 acccggagac caaggtgctc gagccggtgt gcagcgtcac cgccaacttc gccgacggcg
     4201 gcagcgagca ggtccccgcc tgcgtcgtcg acggcgacga gtgggcggtg cccaagggcg
     4261 cctcggtgtg cttcgccatg ctcgtcgacc ccgacggccg cacgcccttc acgggcgacg
     4321 acatgacgcg ggtgacgacc gacggcggcg cgccggcccc ggcgtgcagc accgacaccg
     4381 agaacgtcga gttcaagctc ctgcgcgcgg gtccgcggcg cgctggcgtg tcctacgagg
     4441 ccgcctgcgt cgccgccgac gacaacacca cctgcgccgc cgactgagcg tcaacgctcg
     4501 atgtgccggc gtgcgagcgc gggcggatcg ggctagcatg ggccagcgca tgtcccagtt
     4561 cccctcgctt ctcctgcgac acggcggcgt gtcggcccag ggcgccttcg ccgaggccca
     4621 ggccgccttc ctcgaccccg atcccacgac cgtacagcgc ctgcgttcgc tgctggccgc
     4681 cagcgaggcc ggggtcgtcg cccacttcta catggacgcc gagctgcaag gcgcgctgct
     4741 ggcgacgaag tggcagcgcc tgcacatctc cgactcgctg gtgatggccg accgcgcggt
     4801 ggcgatggcc cagcagggcg cgcgcgcggt ggtcgtgctc ggggtcgact tcatgtccga
     4861 gaacgtgcgc gcggtgatgg actcggccgg cttcggccac gtcccggtgt accgcgtcac
     4921 cgcggaggcg atcggctgct cgctcgccgc cgccgccgag gcgccggcct acgcggccta
     4981 cctgcgccgc gccgcgcaga cgccgaactc gctgcacgtc gtctacatca acaccagcct
     5041 gcgcaccaag gccaacgccc aggcgatcct gccgacgatc acctgcacct cgtcgaacgt
     5101 catccagacg atcctcgaga ccttcgccag cgacccctcg gcgaacgtgt ggttcggccc
     5161 cgacacgtac atggggcaca acctccgcgt gttgttcgag cgcctcgccg agatggacga
     5221 cgccgcgatc gcggcgctgc acccgaagca cgaccgggcc agcgtcgccg ccctgctgcc
     5281 gcgctttcat tacttcgagc agggcaactg catcgtccac cacatgttcg gcgccgaggt
     5341 cgtcgcccgc gtgcgcgacg cctacccgga cgcgcatatc accgcccacc tcgaggtccc
     5401 cggcgagatg ttcgcgctcg ccctcgcggc ccagggccac ggccgcggcg tcgtcggctc
     5461 gacctccgac atcctcggct acatcacgaa gcaggtgaag gccgcccccg cggggcccgg
     5521 ccacctgcaa ttcgtgctcg gcaccgaggc cggcatggtc accgcgatcg tgcacgcggt
     5581 ccgcgagcag ctcgcggccc gccccgagct caccgtcgag atcgtgtttc cggtcgccga
     5641 gggcgccgtg ctcgcgaccg gcgacgccga gctgccgctc gtgcccgggg tcgccggcgg
     5701 cgagggctgc tcgaccgcgg gcggctgcgc gacctgcccg tacatgaaga tgaactcgct
     5761 cgacgccctg atggccgtgc tcgagcgcct gcagaccccc gccgcgctct cggcccacgc
     5821 gccccgcgtc taccgcgagc agatcggcgg ccagacggtc gcccagctcg gcgagctgcc
     5881 gatccgccac atgcgcgact tccagaagtc cggccacctg ccgcccgcgc tcgtcgagga
     5941 catgcgcacc cgagccgcgg ccgcgggctg acctgtccct cgggtcagat cagcgagcgc
     6001 cgcggtcgac ctgcttcggc ttgacatcgc cgttgaggcg gccggtcgcg tacagcacgc
     6061 gagcggtggc gacgcgcagg tcgatgcgcg agttgaccgc gcgcaggccg gcctggacct
     6121 ggtcaccctc ggcgtcgatg atctcgttgg tcgtcgcggc gccggcctgg tagttctcgc
     6181 tggccacgcg gtaggcctcg gccgcggagg ccaggccgcg ggtgctcagc tcgatcgccg
     6241 cgagcgcgcg ctcgcggtcg tggtacgcct gcgcgacctc catcgcgatc ccgcggcgca
     6301 tcatctccgc ctgcagcagc agctcgcgct tgtcggcctt gagctggcgg acctcgacct
     6361 tggtgcgcag cgtctggttg atcgtgtagc tcagctgcac gccgaccgac cacgacccgc
     6421 gccacaccgg cgcgagcggg aagaagcgct ggttggggtt ggcgtacagg gcgtcggcga
     6481 aggcgtcgag ccgcgggtag tagccggcgc gggtcgcccg gatgccctgg tgcgtgccgt
     6541 cgatcgccca ctcgagcgag cgcagctcga ggcggttctg gtgcgcctcg gcgatcagct
     6601 cgtcgacgga gggcaggggg ccgaggtcgg ccggcgcggc gagcaggtcc tcaccgacca
     6661 cgaagtcctt gggcggctcc ttcatcatga tcgacagcga gcgctcggcg atcttgcgat
     6721 acgcgcgggc gtcggcgatc gcggcctgtt gcgacgagac cagggcgtcg acgcgcagca
     6781 cgtcggagcg ggtcgccgag ccggcgtcga acaggttctg cacgtcggcg aggcgcgact
     6841 gggtccgctg cagcgcgtcc tcgatgacga tcagggaggc gacgccgcgc agccagttga
     6901 agtaggccag gcgggcgtcg gtctggatct tgatgcgctc ggcgtcgcgg gccaggatca
     6961 cggactcgcg cgcggcgtgg gtgcccttgc gcgccggcgc gagcgagagg atgtagtcgg
     7021 agatcgggac cgcgatgctg gccgacagcg agtacgagtt gagcgggatc tggatcttga
     7081 acggcggggt gacgaccagg ggcgcgccat tggggtcgag ggcgcactga cccatgcctc
     7141 cagggcactc gccgatcgtc acc
//
LOCUS       DASWNJ010000360         1597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_393912, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000360 DASWNJ010000000
VERSION     DASWNJ010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393912"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="VGB85_06300"
     CDS             complement(<1..1086)
                     /locus_tag="VGB85_06300"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0133669.1"
                     /translation="MEEAGDSSFPNWPEFRRLFAYDNLIGKVLQGRYEIVRRLGAGGM
                     GVVFLARHIHLDKHFALKIISPRFLDEPEIAERFLLEARAASKIDHPNVVSITDFGVP
                     EEGPAFFAMEHLEGEDLAKMLAREGHLPWPRALPIVAQIARALAAAHARGVVHRDIKP
                     QNCFRITRDDNPDFIKVLDFGLAKVLSGTGNSAWTVGGSPGYIAPEIYRGGKVDHRVD
                     IFALGVLLHTLLVGRLPAQDPDPGVAAVPALPPPLRALIEHATAEDLTRRLPTIEAFL
                     AELEATRAALESPAPVQQALARRTRTRWGWIAAAAVTAGVTITLLASQRTSEPTVLTS
                     PGAPATPTPAVAPPDVARDPTPAPEPVP"
     gene            complement(1110..>1597)
                     /locus_tag="VGB85_06305"
     CDS             complement(1110..>1597)
                     /locus_tag="VGB85_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133670.1"
                     /translation="LLAPVAAAAPVPPPGPATSPPSTPATTPPQTTPPARPAPSTDHD
                     GRRERIAGGVALGLGVTGVALLLTGVGLDRRANARGHALCREAMTGCTAYGSQLRDIE
                     VLGRRGDTLLGVGAVLGGLGLVSGAVLMAIGLRRRDASRVSWAPRITPSSAGLALSGR
                     F"
BASE COUNT          211 a          506 c          680 g          200 t
ORIGIN      
        1 cggaacaggt tcgggcgcgg gcgtcgggtc cctggcgacg tcgggcggtg ccaccgcggg
       61 tgtcggcgtc gcgggtgcgc cgggcgacgt cagcacggtc ggctcggacg tgcgctggga
      121 cgcgagcaga gtgatcgtca cgccggcggt gaccgctgcc gccgcgatcc agccccagcg
      181 tgtgcgtgta cgtcttgcga gcgcctgctg gaccggcgcg ggcgactcga gggcggcgcg
      241 ggtcgcctcg agctcggcga ggaacgcctc gatcgtcggc aggcggcgcg tgaggtcctc
      301 ggcggtcgcg tgctcgatca gcgcccggag cggcggcggg agggcgggca cggcggccac
      361 gcccgggtcg gggtcctgcg cgggcaggcg gccgacgagc agggtgtgca gcaggacccc
      421 gagggcgaag atgtcgacgc ggtggtcgac cttgccgccg cgatagatct ccggggcgat
      481 gtagccgggc gatccgccga cggtccacgc gctgttgccg gtgcccgaga ggaccttggc
      541 gagcccgaag tcgaggactt taataaaatc ggggttgtcg tcgcgggtga tgcgaaagca
      601 gttctgcggc ttgatgtcgc gatgcacgac gccgcgcgcg tgggcggccg cgagggcgcg
      661 ggcgatctgg gcgacgatcg gcagggcgcg cggccacggg aggtggccct cgcgggcgag
      721 catcttcgcc aggtcctcgc cctcgaggtg ctccatggcg aagaaggcgg ggccctcctc
      781 gggcacgccg aagtcggtga tgctgacgac gttggggtga tcgatcttcg aggcggcgcg
      841 ggcttcgagc aggaagcgct cggcgatctc cggctcgtcg aggaagcgcg ggctgatgat
      901 cttgagggcg aagtgcttgt cgaggtggat gtgccgggcg aggaacacca cgcccatgcc
      961 gccggcgccg aggcgacgca cgatctcgta gcggccctgc aggaccttgc cgatcaggtt
     1021 gtcgtaggcg aagaggcggc gaaactccgg ccagttcggg aacgagctgt cgccggcttc
     1081 ttccacgccc ggcgcgagac tagcacgcgt cagaagcggc ccgacagggc caggccggcg
     1141 gacgaggggg tgatacgcgg ggcccacgag acgcgggagg cgtcgcgcct gcgcaggccg
     1201 atcgccatca gcacggcgcc ggagaccagc ccgaggccgc cgaggacggc cccgacgccg
     1261 agcagggtgt ccccgcggcg gccgaggacc tcgatgtcgc gcagctgcga gccatacgcg
     1321 gtgcagccgg tcatcgcctc gcggcacagc gcgtgcccgc gggcgttggc ccggcggtcg
     1381 agcccgacgc cggtgagcag cagggcgacg ccggtgacgc cgaggccgag cgcgacgccg
     1441 ccggcgatcc gttcgcgccg gccgtcgtgg tcggtcgagg gagcggggcg cgcagggggc
     1501 gttgtctgcg gcggggtcgt ggcgggcgta ctggggggtg aggtggcggg cccgggcggt
     1561 ggcacggggg ccgcggcggc gacgggcgcg agcaggg
//
LOCUS       DASWNJ010000361         2531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_183380, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000361 DASWNJ010000000
VERSION     DASWNJ010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183380"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1788
                     /locus_tag="VGB85_06310"
     CDS             <1..1788
                     /locus_tag="VGB85_06310"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0133671.1"
                     /translation="AASVAVLADDLTPDDPVELQEAAAFSIGRLGRRKVALDEATRTR
                     LAKLRAPELAEYYALANNPAPPGELRRDLATALLTAGTTDIDPTVRRLAYTGLLARYP
                     AQTRLGDCIVHALHPRYDDDAFAFQADISDEQALTLLQAWGAKLFHLPRMNRRGELVT
                     TRIPPPIDPSVWPDGPRRLLAGLACLAAAKPELLTPARREHLVTLRSAAREADARVVA
                     ETKVERPLSAMLASHKTLAQLDCRLAQLLARGPAWRPPLTCASPGDASLLEPAILAAG
                     FGGPDRLARLDELLRTRDPAVQIAAITAAATLWDDPAAAKSVQRWLLAALDAPTLGVI
                     GAAADAITARHKQDAPPPITADDPLWRTLARRARGALAHEPELYATLVATLAATRVPA
                     GVGVCEPGLRHANYSVREAARACIKALRGADPGPQAPGGPQAVPPVDPGRLAGQRVLW
                     TLTTEYGDLKIDLDPDAAPWAVATIVALTGRGFYDGLSFHRDVPGFVLQGGDPEGTGW
                     GGPGFVLPSEPSHHRFDRGAVGIADAGKDTGGSQFFFMHDRAPHLEGRYTWVGQLRPG
                     QHGLLPLLTVGDRILKATVKLRPPRARPR"
     gene            complement(1798..>2531)
                     /locus_tag="VGB85_06315"
     CDS             complement(1798..>2531)
                     /locus_tag="VGB85_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133672.1"
                     /translation="TEPTTGDTTVDTTGDTDATDTETTGAPVFPMCEEPTGPDNIGFE
                     MSGEGLPLGVELNLECTIISTQTSPEMTATIDLSCTDGMVLEVEVTIDLQLAYEPNLG
                     DLGATKVRLRHRKIDSFEQREVLALHDFDNDDVLLLFAARGYDIFTIEDKALWAPLQI
                     TPVAEALCATEGECEQVTRSALDITLDEITERVFDRDFFSFGPPGPSVHVGSARVIDL
                     YALPECDPGDGADGFETNLVVIAEQ"
BASE COUNT          336 a          988 c          856 g          351 t
ORIGIN      
        1 gccgcctcgg tcgcggtgct cgccgacgac ctcacccccg acgacccggt ggagttgcaa
       61 gaggctgccg cgttttcgat cggtcgcctc ggtcgccgca aggtcgcgct cgacgaggcg
      121 acgcggacac gcctggcgaa gctgcgagcc cccgaactgg ccgagtatta cgcgctcgcc
      181 aacaacccgg ccccgccggg cgagctgcga cgcgacctcg ccacggcctt gttgacggcg
      241 ggcacgaccg acatcgaccc gaccgtgcgg cggctggcct acaccggcct gctcgcgcgc
      301 tacccggcgc agacgcgcct gggcgactgc atcgtccacg ccctgcatcc gaggtacgac
      361 gacgacgcgt tcgccttcca ggccgacatc tccgacgagc aggcgctgac gctgctgcag
      421 gcgtggggcg cgaagctctt ccacctgccg cgcatgaaca ggcgcgggga gctcgtcacg
      481 actcgcatcc cgccgccgat cgacccctcc gtgtggcccg acgggccgcg acggctgctc
      541 gcgggcctcg cctgcctcgc agccgccaag cccgagctgc tgaccccggc gcggcgcgag
      601 cacctcgtca ccctgcgtag cgcggcccgc gaggccgatg cccgcgtcgt cgccgagacg
      661 aaggtggagc ggccgttgag cgccatgttg gcgagccaca agacgctcgc tcagctcgac
      721 tgccggctcg cacaattgct agcccgcggg ccagcatggc ggcccccgct gacatgtgcc
      781 tcgccgggcg acgcgtcgct gctcgagccg gcgatcctcg ccgcgggctt cggcggaccg
      841 gatcgcctgg cccgcctcga cgagctgctg cgcacgcgcg accctgcggt ccagatcgcc
      901 gcgatcacgg ccgcggcgac cctgtgggac gaccccgccg ccgcgaagtc ggtgcagcgc
      961 tggttgctcg ccgcgctcga cgctccgacg ctcggcgtca tcggggccgc ggccgacgcg
     1021 atcacagccc gtcacaagca ggacgcgcca ccaccgatca ccgccgacga cccgctgtgg
     1081 cgcacgctcg cgcgacgggc ccgcggcgcc ctcgcccacg agcccgagct ctacgcgacg
     1141 ctcgtcgcca ccctcgccgc cacccgcgtg cccgccggcg tcggcgtctg cgagcccggc
     1201 ctgcgtcacg ccaactacag cgtccgcgag gccgcacgcg cctgcatcaa ggcgctacgc
     1261 ggcgcggatc ctggcccgca ggcccccggg gggccccagg ccgtcccgcc tgtcgatccc
     1321 gggcgcctgg ccggccagcg cgtgctgtgg acgctgacca ccgagtacgg cgacctgaag
     1381 atcgacctcg accccgacgc cgcgccgtgg gcggtcgcca cgatcgtcgc gctgaccggg
     1441 cgcggcttct acgacggcct gagctttcac cgtgacgtgc ccggcttcgt gctgcaaggc
     1501 ggcgaccccg agggcaccgg ctggggcggg cccggctttg tcctgccctc cgagccctcg
     1561 caccaccgct tcgatcgcgg cgcggtcggc atcgccgacg ccggcaagga caccggcggc
     1621 agccagttct tcttcatgca cgaccgcgcc ccgcacctcg agggccgcta cacctgggtc
     1681 ggccagctgc gaccgggcca gcacggactg ctgccgctgc tgaccgtcgg cgaccggatc
     1741 ctcaaggcga ccgtgaagct ccggccgccg cgcgcccggc cgcgatgatc cccgcgctca
     1801 ctgctcggcg atcacgacga ggttcgtctc gaagccatcg gccccgtcgc cggggtcgca
     1861 ctccggcagc gcgtagaggt cgatcacccg cgccgagccg acgtgcaccg acggcccggg
     1921 aggtccgaag ctgaagaagt cgcggtcgaa cacccgctcg gtgatctcgt cgagggtgat
     1981 gtcgagcgcg ctgcgcgtga cctgctcgca ctcgccctcg gtcgcgcaca gggcctccgc
     2041 gaccggcgtg atctgcaggg gggcccacag cgccttgtcc tcgatcgtga agatgtcgta
     2101 gccgcgcgcg gcgaacagca gcagcacgtc gtcgttgtcg aagtcgtgca gcgccagcac
     2161 ctcgcgctgc tcgaagctgt cgatcttgcg atgccgcagg cgcaccttgg tggcgccgag
     2221 gtccccgagg ttcggctcgt acgcgagctg gaggtcgatc gtgacctcga cctcgagcac
     2281 catcccatca gtgcacgaca ggtcgatcgt cgccgtcatc tcggggctcg tctgcgtgga
     2341 gatgatcgtg cactcgaggt tgagctcgac gccgagcggc aagccctcgc ccgacatctc
     2401 gaagccgatg ttgtccggac ccgtcggctc ctcgcacatc gggaacaccg gcgccccggt
     2461 cgtctcggtg tccgtcgcgt cggtgtcgcc cgtggtgtcg acagtggtgt cgcccgtcgt
     2521 cggctccgtg g
//
LOCUS       DASWNJ010000362         2233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_147680, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000362 DASWNJ010000000
VERSION     DASWNJ010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147680"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VGB85_06320"
     CDS             complement(<1..292)
                     /locus_tag="VGB85_06320"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEY0133673.1"
                     /translation="MLTKKKNRQQPSATTESSLMGSVAPEFLFHPEERVAVFIDGANL
                     YQAARGIGFDIDYKRLLRAFSYDCRLIRAYYYTALLDSQEYSPVRPLVDWLDY"
     gene            473..841
                     /gene="rpoZ"
                     /locus_tag="VGB85_06325"
     CDS             473..841
                     /gene="rpoZ"
                     /locus_tag="VGB85_06325"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEY0133674.1"
                     /translation="MARITVEDCVLRVPSRFELVMLAAQRARDISAGAPLGVDRDNDK
                     NPVVALREIAEGTADLDHLRYELVHGLRRHLDAAGGNEPPMDLAEELEALIPGQDAAG
                     VVAPMPEGVSYGEHDVADAD"
     gene            906..>2233
                     /locus_tag="VGB85_06330"
     CDS             906..>2233
                     /locus_tag="VGB85_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY0133675.1"
                     /translation="MDLAVAAGDVMLADEAPSVMAAIAAGAPVPPAAAVEPPATPRPP
                     RRPVMRQYELVERVKAHDPDVDEDLLNRAYVFAMKAHGSQLRASGDPYFHHPVEVAGI
                     LTGYRVDSASIATALLHDTVEDTGATLEEIERLFGRTVARLVDGVTKLNKLELQSAQT
                     AQAENFRKLLLAMSDDIRVLLVKLADRLHNMRTLGFIKAPEKRRRIAAETMEIYAPLA
                     ERIGMEGLKAELDDLAFAELFPEARESVIHRLRHLRQGDVDLVERVCAGLRRTFEEGG
                     IAAEVAGREKTPHSIWRKMERKNVAFEQLSDIMAYRVTVASVGDCYAALGALHGAYHT
                     LPGRFKDYISTPKSNGYRSLHTTIIGPENRKIEIQIRTREMHEQAELGVAAHWAYKQA
                     GGTTEGRQYRWIRELLDILEHAAGPEEFLEHTKLEMFQDQVFCFTPKGDL"
BASE COUNT          383 a          770 c          777 g          303 t
ORIGIN      
        1 tgtagtcgag ccaatcgacc aggggacgga ccggcgagta ctcctgcgag tcgagcaatg
       61 ccgtgtaata gtaggcgcgg atgagacggc aatcatacga gaaggcgcgt agcaaccttt
      121 tgtagtcgat gtcgaagccg attcctcgtg cagcttgata caggttcgcg ccgtcgatga
      181 agaccgccac ccgctcctcg gggtggaaca agaactccgg ggcaacgctg cccatcaggg
      241 atgactccgt ggttgctgaa ggctgttgtc tgttcttctt tttggtgagc acggcccacg
      301 gaccttggca aggccggccg cacccccagc gtcggaggca atctagcgca ccaccaagcg
      361 cacacgccac aacgcgatcg acgttccggc gcctgccggg tttgcgccgg acgcctacct
      421 ccgctacatg taggggaaat ctgcgcttcc caccctcccc ggagagaaat cgatggctcg
      481 catcaccgtc gaagactgcg tgctgcgcgt gccctcccgg ttcgagctcg tcatgctggc
      541 cgcccagcgc gcccgcgaca tctcggccgg ggcgccgctc ggcgtcgacc gcgacaacga
      601 caagaacccg gtcgtggcgc tcagggagat cgccgagggc acggccgacc tcgaccacct
      661 ccgctacgag ctggtccacg gcctgcgccg ccacctcgac gccgccggcg ggaacgagcc
      721 gccgatggat ctggccgagg agctcgaggc gctgatcccg ggccaggacg cggccggggt
      781 cgtggcgccg atgccggaag gcgtgagcta cggcgagcac gacgtcgcgg acgcggactg
      841 agggcgcgct gacggcggcg gcggcggccg gggcggaggc cggagcgggc aggggaggtc
      901 gagccttgga ccttgcggtg gcggcgggtg acgtgatgct ggcggacgag gcgccgtcgg
      961 tgatggccgc catcgcggcg ggcgcgccgg tgccgccggc tgcggcggtc gagccgcccg
     1021 cgacgccccg accgccgcgc cggccggtca tgcgccagta cgagctggtc gagcgcgtca
     1081 aggcccacga cccggacgtc gacgaggacc tgctcaaccg ggcctacgtc ttcgcgatga
     1141 aggcgcacgg cagccagctc cgcgcctcgg gcgaccccta cttccaccac cccgtcgagg
     1201 tcgccggcat cctcaccggc taccgggtcg actcggcctc gatcgcgacg gcgctgctcc
     1261 acgacacggt cgaggacacc ggcgccacgc tcgaggagat cgagcggctg ttcgggcgca
     1321 cggtcgcccg gctggtcgac ggcgtgacca agctgaacaa gctggagctc cagtcggcgc
     1381 agaccgcgca ggcggagaac ttcaggaagc tgctgctcgc gatgtcggac gacatccgcg
     1441 tgctgctggt gaagctcgcc gaccgcctgc acaacatgcg cacgctgggc ttcatcaagg
     1501 cgcccgagaa gcgccggcgg atcgccgccg agacgatgga gatctacgcg ccgctggccg
     1561 agcggatcgg catggaaggc ctcaaggcgg agctcgacga cctggccttc gcggagctgt
     1621 tccccgaggc ccgcgagagc gtcatccacc ggttgcggca cctgcgccag ggcgacgtcg
     1681 acctcgtcga gcgggtctgc gccggcctga ggcgaacctt tgaggagggc gggatcgccg
     1741 ccgaggtcgc gggccgcgag aagacgcccc actccatctg gcgcaagatg gagcgcaaga
     1801 acgtcgcctt cgaacagctc tccgacatca tggcgtaccg ggtcacggtc gcctcggtgg
     1861 gcgactgcta cgcggccctc ggcgcgctgc acggcgccta ccacacgctg cccggccgct
     1921 tcaaggacta catcagcacg ccgaagtcga acggctaccg gtccctgcac accaccatca
     1981 tcgggccgga gaaccgcaag atcgagatcc agatccgcac ccgcgagatg cacgagcagg
     2041 ccgagctcgg cgtggccgcg cactgggcct acaagcaggc gggcgggacc accgagggcc
     2101 ggcagtaccg ctggatcagg gaactactcg acatcctgga gcacgcggcc gggccggagg
     2161 agttcctcga gcacacgaag ctcgagatgt tccaagacca ggtgttctgc ttcacgccca
     2221 agggcgacct gat
//
LOCUS       DASWNJ010000363         6618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_430898, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000363 DASWNJ010000000
VERSION     DASWNJ010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6618
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430898"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..885)
                     /locus_tag="VGB85_06335"
     CDS             complement(118..885)
                     /locus_tag="VGB85_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133676.1"
                     /translation="MIAAPLLPLVLSAFSALAPAEALDPEAKEHFDSGITSYKAKEYA
                     AAIKELRTAYALDPQPVILFAWAQAERLYGRCSRADKLYKRFLKTRPSEQQTEAARQG
                     IDRCKGQPDIASDDVDEQEAAKEAAPAPVAETVEEAPTPEPEPETVKPKRRIDGVGIG
                     MLAGGVVLGAVGTGLLASGQSKAKAADGASSYDEYADDASQVRKLRITGGVLAGVGGA
                     LILAGVVKLVMHARQPRHDVSFWSAPTGGGLIVRLRF"
     gene            complement(882..2387)
                     /locus_tag="VGB85_06340"
     CDS             complement(882..2387)
                     /locus_tag="VGB85_06340"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0133677.1"
                     /translation="MNSVAAPARPRDSQTALVRLGKYELLTRLAVGGMAELFVARTLA
                     QHGFEKLVALKRILASHADDENFIRMLLAEARLAATLHHPNIAQVYDVGDDNGTFFFT
                     MEYVVGQDLRQIVRAIQAKGEWLTPEHILQIVIGTAAGLHYAHDKEDGDGNPLGIVHR
                     DVSPSNILVSYDGSVKLVDFGIARVTALQTNTGLGALKGKVPYMSPEQCRGEALDRRS
                     DVFSLGIILWELSLRRRLFSGDNDLVVAGKVCNEDAPAPTSIDPDYPPELEAIVLKAL
                     ARDRDQRWASAQELQLALEQYAFDRHLLLSSAKLGSFMSELFADLIRKTKANLREKIA
                     SSTGLFPIPPDVIAASASASSRPAATLHVNEATTSGLIPLPDDRDSEQALTRPEGQRE
                     ATRRPPWLWIAGGGVAAAILLMISLGGDPPEPAPSASAAAPVIAEPPAPVVTPPVVVA
                     PSVPAVQSSPEVPATAPAKSTPTPAAKKKSGKSRTTKKSTNWDPDSPILPP"
     gene            complement(2571..3410)
                     /locus_tag="VGB85_06345"
     CDS             complement(2571..3410)
                     /locus_tag="VGB85_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0133678.1"
                     /translation="MNVPLSAEPLILVTGSTDGIGRETARELTRRGARVLVHGRDPAR
                     ARATVEELRGLGGSPPEPVIADLSSLAAVRAMAAQMAHDCHQPEVIIHNAGVFMKQRE
                     RTVDGFEMTLAINHLAPFLLTHLVLESTVGAHLRRIVNVSSMAHNRGRIDFDDLQLLR
                     EAFNSYASYATSKLANVLFTVELARRVAFRRVTVNALHPGVTSTKLLTEGFEMEGHDS
                     LAESAATSVTLALDPGLVEATGGYYVQSKPAPPHPVAQDRDVARRFYEVSAEMTGVMP
                     LPA"
     gene            3521..3955
                     /locus_tag="VGB85_06350"
     CDS             3521..3955
                     /locus_tag="VGB85_06350"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEY0133679.1"
                     /translation="MHIPLLCLLGFVSWTVLIVVVGIAGVRFTAIATGTAPPNGFPAD
                     VPHGSDRYRRTMRAHINSVENLPLFASVVLTGAVVHAGSPLMDTLALTYLGARVFHTL
                     FHVASGRGLGIALRLTAYVVQLVCLSWMGVLVARVILASKLV"
     gene            complement(3961..4875)
                     /locus_tag="VGB85_06355"
     CDS             complement(3961..4875)
                     /locus_tag="VGB85_06355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEY0133680.1"
                     /translation="MSGDGPVPGARPVEQASATEAAKFPAPQIYLGDNLPILAGLSAA
                     SFDLIYVDPPFNTGRTQARTQIQVVQDTDGDRTGFSGRRYRTIKLGTRAYADVFDDYI
                     EFLWPRLEQAHRLLTPRGSFFLHLDYREVHYAKVALDQIFGRAGFMNEIVWAYDYGAR
                     SRSRWSAKHDNILWYVKDPGDYTFEYDAIDRIPYMAPGLVGPVKAARGKTPTDTWWST
                     IVSPNGREKTGYPTQKPLKVIERIVRVHSRAGDRLLDFFAGSGTFGEAAVRLGRQVTL
                     IDHNPEAIEVMRRRLEFAAPVIFAPAEP"
     gene            complement(4872..5486)
                     /locus_tag="VGB85_06360"
     CDS             complement(4872..5486)
                     /locus_tag="VGB85_06360"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY0133681.1"
                     /translation="MRIHIEPERPNPRKLQPAVDALRRGQVIIYPTDTGYAFGCALSS
                     AKGIASIRKLKGLDERSHKPLTMIVKDLAEVGRYGVMSNSHFRLIRRLLPGPYTLILK
                     AASDVPRSMYNRNHEVGIRMPDHVVANLLVGELGEPLLTGSVTPYDDNLPELEEPETY
                     EQRHAREVAVVVDIGALWPDPSTVLRFVDGEVEVVRQGKGEFTP"
BASE COUNT          930 a         2287 c         2324 g         1077 t
ORIGIN      
        1 tcgccgtttt gacactccga gctctgcgcg caggcgaagc tgtcgagctt gacgcaacca
       61 ccggcagcga gtgcgacgag cgcgacgatg gcacggccga gggtatgcat gatcgattca
      121 gaagcgcagg cgaacgatga ggccgccgcc ggtcggggcg gaccagaacg acacgtcgtg
      181 ccgcggctgg cgagcgtgca tgacgagctt gacgaccccc gcgaggatca gcgcaccacc
      241 gacaccggcg agcacgcccc cggtgatccg gagcttgcgg acctggctgg cgtcgtcggc
      301 gtactcgtcg tagctgctgg caccatcggc ggccttggcc ttgctctgcc cgctcgcgag
      361 caggccggtc ccgaccgcgc cgagcacgac gccgccagcg agcatgccga tgcccacgcc
      421 gtcgatgcgg cgcttcggtt tcaccgtctc gggctcgggc tcgggcgtcg gggcctcctc
      481 gaccgtctcg gccaccggcg cgggcgcggc ctccttcgcg gcctcctgct cgtcgacgtc
      541 atcgctcgcg atgtcgggtt ggcccttgca gcggtcgatg ccctggcgcg cggcctcggt
      601 ctgctgctcc gaggggcgcg tcttgaggaa gcgcttgtag agcttgtcgg cgcggctaca
      661 gcggccatac aggcgctcgg cctgggccca cgcgaacagg atcaccggct gggggtcgag
      721 cgcgtaagcg gtgcgcagct ccttgatcgc ggccgcgtac tccttggcct tgtagctggt
      781 gataccgctg tcgaagtgct ccttcgcctc ggggtcgagc gcctcggcgg gcgcgagcgc
      841 ggagaaggcg gacaggacca gtggaaggag cggcgcggcg atcatggcgg gaggatgggc
      901 gagtcgggat cccagttggt cgacttcttc gtggtcctcg acttcccgga cttcttcttc
      961 gccgccggcg taggcgtcga ctttgccggt gcggtcgccg ggacctccgg cgacgattgc
     1021 acggcgggca ccgacggcgc caccacgacc ggcggcgtca cgaccggggc cggcggttcg
     1081 gcgatcacgg gcgccgcagc agatgcagac ggggcgggct cgggcgggtc gccgccgagc
     1141 gagatcatca gcagaatcgc cgcagccacc ccaccgccgg cgatccacag ccacggcggc
     1201 cggcgggtgg cctcgcgttg gccctccggg cgggtcagcg cctgctcact gtcgcggtcg
     1261 tccggcagcg ggatcaggcc cgaggtggtc gcctcattga cgtgcagcgt cgccgccggc
     1321 cggctcgagg ccgacgcgct cgcggcgatc acgtccgggg ggatcgggaa caggccggtg
     1381 ctcgacgcga tcttctcgcg caggttcgcc ttggtcttgc ggatcaggtc ggcgaacagc
     1441 tcgctcatga agctgccgag cttggccgac gacagcagca ggtggcggtc gaacgcatac
     1501 tgctcgagcg cgagctgaag ctcctgcgcg ctcgcccacc gctggtcgcg gtcacgcgcg
     1561 agcgccttga gcacgatcgc ctcgagctcg gggggatagt cggggtcgat cgacgtcggc
     1621 gccggcgcgt cctcgttgca gaccttgccc gcgacgacga ggtcgttgtc gccgctgaac
     1681 agccggcgac gcagcgacag ctcccagagg atgatgccga gcgagaacac gtcgctgcgg
     1741 cggtcgagcg cctcgccccg gcactgctcc ggcgacatgt acgggacctt gcccttgagc
     1801 gcgccgagcc cggtgttggt ctgcaacgcg gtgacgcggg cgatgccgaa gtcgacgagc
     1861 ttgacgctgc cgtcgtagct gaccaggatg ttcgacggtg agacgtcgcg gtggacgatg
     1921 ccgagcgggt tgccgtcgcc gtcctccttg tcgtgcgcgt agtgcagacc ggcggcggtg
     1981 ccgatgacga tctgcaaaat gtgctcgggc gtcagccact cgcccttcgc ctggatcgcg
     2041 cggacgatct ggcgcaggtc ctggccgacg acgtactcca tcgtgaagaa gaacgtgccg
     2101 ttgtcgtcgc cgacgtcgta cacctgcgcg atgttggggt gatgcagcgt cgccgccagg
     2161 cgagcctccg ccagcaacat ccggatgaag ttctcgtcgt ccgcgtgcga cgcgaggatc
     2221 cgcttgagcg cgaccagctt ctcgaagccg tgctgcgcca gcgtccgcgc gacgaacagc
     2281 tccgccatgc caccgaccgc gagccgcgtc agcagctcgt acttgccgag ccgtacgagc
     2341 gccgtctgcg agtctcgcgg cctcgctggt gcagcaacag agttcatcgg cggggattat
     2401 aggcagcccc ccggtgcgac gatagacgca tgtaggcaga cctccctggc gatgtcgcgc
     2461 ggcgacgcga gaaaccagag gtttggcggc ccccgccctg cacccggcgc gccgccgtcg
     2521 ggggcgcctg tctccggccg gagatcaccc cggcactacg ccacgctcgc tcacgccggc
     2581 aagggcatca cgcccgtcat ctcggcgctg acctcgtaaa accggcgggc gacgtcccgg
     2641 tcctgggcga ccggatgcgg cggcgcaggc ttgctctgaa cgtagtaccc gccggtcgcc
     2701 tcgacgaggc ccgggtcgag cgcgagggtc acggaggtcg cggccgactc ggcgagcgag
     2761 tcgtggccct ccatctcgaa gccctcggtc agcagcttgg tcgaggtgac gcccgggtgc
     2821 agggcgttga cggtgaccct gcgaaacgcg acgcgccgcg cgagctcgac ggtgaacagc
     2881 acgttggcca gcttggaggt cgcgtagctg gcgtagctgt tgaacgcctc gcgcagcagc
     2941 tggaggtcgt cgaagtcgat gcggccgcgg ttgtgggcca tcgagctgac gttgacgatc
     3001 ctgcgcaggt gcgcgccgac ggtgctctcg agcaccaggt gtgtcagcag gaacggcgcg
     3061 aggtggttga tcgccagcgt catctcgaag ccgtcgaccg tgcgctcgcg ctgcttcatg
     3121 aacacgcccg cgttgtgaat gatgacctcc ggctggtggc agtcgtgggc catctgcgcc
     3181 gccattgcgc ggaccgcggc gaggctcgag aggtcggcga tcaccggctc gggcgggctc
     3241 ccgccgagcc ctcgcagctc ctccaccgtg gcgcgcgcgc gcgcgggatc tcggccgtgg
     3301 accagcaccc gggcgccccg gcgggtcaac tcgcgggccg tctcgcggcc gatcccatcg
     3361 gtggatccgg tgacgaggat gagcggctcg gccgagagcg gtacattcat cacccaaacg
     3421 tgtcgcggat cgccccgcct gtcgagcggc cggggcggat gtatgtgcag ccgcccgaac
     3481 gcccgcgttc gccggcccgc tggcggtacc atggcccgcc atgcacatcc ccctgctctg
     3541 tctcctcggc ttcgtgtcct ggaccgtgct catcgtcgtc gtcggcatcg ccggcgtccg
     3601 cttcaccgcg atcgcgaccg gcacggcccc gcccaacggc ttccccgccg atgtcccgca
     3661 cggcagcgac cgctaccgcc ggaccatgcg cgcgcacatc aacagcgtcg agaacctgcc
     3721 gctgttcgcg tcggtcgtgc tcaccggcgc ggtcgtccac gccggctcac cgctgatgga
     3781 caccctggcg ctgacctacc tcggcgcccg cgtctttcac acgctgttcc acgtcgcctc
     3841 gggtcgcggc ctcgggatcg ccctgcgact gacggcgtac gtcgtccagc tggtgtgcct
     3901 gagctggatg ggcgtgctgg tcgcccgcgt gatcctggcg agcaagctcg tgtgatcgcc
     3961 tcacggctcc gcgggggcga agatcaccgg cgccgcgaac tcgaggcgcc ggcgcatgac
     4021 ctcgatcgcc tcggggttgt gatcgatgag cgtcacctgc cggccgagcc gcaccgccgc
     4081 ctcgccgaag gtccccgaac cggcgaagaa gtcgagcagg cgatcgcccg cgcgcgagtg
     4141 cacccgcacg atccgctcga tcaccttcaa tggcttctgc gtcggatagc cggtcttctc
     4201 gcgcccgttc gggctgacga tcgtcgacca ccaggtgtcg gtcggcgtct tgccgcgcgc
     4261 cgccttgacc ggcccgacca gccccggcgc catgtagggg atcctgtcga tggcgtcata
     4321 ctcgaaggtg tagtcgccgg ggtccttgac gtaccacagg atattgtcgt gcttggccga
     4381 ccaccggctg cgcgagcggg ccccatagtc gtaggcccac acgatctcgt tcatgaagcc
     4441 cgcgcgcccg aagatctggt cgagcgccac cttggcgtag tggacctcgc ggtagtcgag
     4501 gtggagaaag aagctgcccc gcggcgtcaa caggcggtgc gcctgctcga gccgcggcca
     4561 caggaactcg atgtaatcgt cgaacacgtc cgcgtacgcg cgcgtcccga gcttgatcgt
     4621 gcgatagcga cgcccggaga agccggtgcg gtcgccgtcg gtgtcctgca cgacctggat
     4681 ctgcgtgcgc gcctgcgtgc gcccggtgtt gaagggcggg tcgacgtaga tcaggtcaaa
     4741 ggacgccgcc gacagcccgg cgaggatcgg caggttgtcg ccgaggtaga tctgcggcgc
     4801 cgggaacttc gcggcttcgg tcgcgctcgc ttgctcgacg ggcctggccc ccgggacagg
     4861 cccgtcgccg ctcacggcgt gaactccccc ttgccctgac gcacgacctc gacctcgccg
     4921 tcgacgaagc gcagcacggt cgaggggtcc ggccacagcg cgccgatgtc gacgacgacg
     4981 gcgacctccc gcgcgtgccg ctgctcgtag gtctcgggct cctcgagctc tggcaagtta
     5041 tcgtcgtagg gcgtgacgga gccggtcagc agcggctccc cgagctcgcc gaccagcaag
     5101 ttggcgacca catgatcggg catgcggatc ccgacctcat ggttgcggtt gtacatgctg
     5161 cgcggcacgt ccgaggcggc cttgaggatc agcgtatacg gccccggcaa caggcgacgg
     5221 atcaggcgaa agtgcgagtt gctcatcaca ccatagcgcc cgacctcggc gaggtccttg
     5281 acgatcatcg tcaacggctt gtgtgaccgc tcatcgagac ccttgagctt gcggatcgac
     5341 gcgatcccct tggcgctgga gagcgcgcag ccgaacgcgt aacccgtgtc ggtcggatag
     5401 atgatcacct ggccgcgacg cagggcgtcg accgcgggct gcagcttgcg cggattgggt
     5461 cgctcgggct cgatatgaat acgcatcaga cttcagtctc tggctctcgg gtgtcgcccg
     5521 ctcgtcgggc tttttggctg tcgcgtgttc ggggaacgcg gggtgctcgg gcagagctgt
     5581 cgcgtgttcg gggaacgcgg agtgctcggg cagaactgtc gcgtgttcgg ggaactcggg
     5641 gtgctcgggc agggctgtcg cgtgttcggg gaacgtgggg tgctcgggca gtgctgtcgc
     5701 gtgttcgggg aacgcggggc gaggctacat gggccgggga ggggccgacg gactgcgggg
     5761 ccctgcgacg cgggtcctcc cagggcacgt gctcgccgcc ccacctgtcc ggaagaacat
     5821 gtctctttca ctgcctctgt cttgccgctc tcgactggcg gtggggcgtc tgcgcgcgag
     5881 ccctgggtcc tcccgctgct tcccccgccg ccggcccctg ctccccgggc gggcattgtc
     5941 tgcgccccgc ttttcgccgg acaagcgatg tctcggtccc gcacgccaac aatctggcgc
     6001 gacgcgagca tcgtccgcgc ctcgctcctc gccgtgcacg cgacttctcg gtctcgcacg
     6061 ccaacaatct cgcgcgacgc gagcatcgtc cgcgtctcgc tattcgccgt gcacgcgact
     6121 tctcggtccc gcacgccaac aatctcgcgt gacgcgagca tcgtccgcgc ttcgctcctc
     6181 gccgtgcgcg cgacttctcg gtcccgcgtc tcaacgatct cgcgcgacgc gagcaacatc
     6241 cggttctcgc tcctcgccgt gcccgcgact tctctgtccc gcgtctcaac gatctcgcgc
     6301 gaaacgagta tcgtcggtgt ctcgcttatc gcagccgtgc acgcaagctc tcgatctcgg
     6361 tgcattcgag ttgcagccgg ggtcgcggtg acgagccgcc ccggacgcgc gaagcttcgc
     6421 acgttcgcga caagcggggc gcatacggtg tccgcccggg gggcaggggc cgactggcgg
     6481 gggaagcagc gggaggaccc agggctcgcg cgcagacgcc ccaccgccgc gcacgagcgg
     6541 agagacagaa gaggtgaaag aagtatgtcc ttccggacag gtggggcggc gagcacgtgc
     6601 cctgggagga cccgcgtc
//
LOCUS       DASWNJ010000364         1672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_7152, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000364 DASWNJ010000000
VERSION     DASWNJ010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7152"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="VGB85_06365"
     CDS             complement(<1..1025)
                     /locus_tag="VGB85_06365"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEY0133682.1"
                     /translation="MREYILVFLVAAAATYLLTVVAREFALRTKAFAVVRDRDVHDEP
                     IPYLGGLAMLGGLVAAYLIGRELPFLSRADPYIFDQARAVLIAGAVICTVGVLDDIFD
                     LDALTKFGGQVLAAGILVWQGVQFFYVPMPDGGALTIDPTQGALLTVFIVVATVNAVN
                     FVDGLDGLAAGVVGVGAVAFFIFCYVLTARNDVTLATTGALLSAALAGACTGFLPHNF
                     HPARLFMGDSGSMLIGLVLAASTITITGQFSADDLGNVDARASLLPILLPISILVVPL
                     ADLVLAVFRRTRAGRSPFAPDKQHLHHRMLEFGHSHRRAVLIMWLWAGLIGSNIVLLG
                     LYSGRTM"
     gene            complement(1035..>1672)
                     /locus_tag="VGB85_06370"
     CDS             complement(1035..>1672)
                     /locus_tag="VGB85_06370"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY0133683.1"
                     /translation="REAAIEAAALAIRRGGLVVLPTDTVYGVGADAFDEDAVAALLEA
                     KGRGRDMPPPVLISSATTADALVLALPGYARTLMEHFWPGPLTLVGKQQPSLQWDLGD
                     TRGTVAIRMPDHPIALELLERTGPLAVSSANFTGRPAATDAVMAEEMLGGRVSVILDA
                     GDSPRGGDAVGSTILDVTGVRPRILRRGALAFEELNAVLESAGAGLLLDEG"
BASE COUNT          282 a          558 c          631 g          201 t
ORIGIN      
        1 cacatggtgc ggccgctgta gaggccgagg aggacgatgt tcgacccgat caaccccgcc
       61 cacagccaca tgatcagcac cgcgcggcgg tgtgagtggc cgaactccag catccggtgg
      121 tggagatgct gcttgtccgg agcgaacggc gacctgcccg cacgggtgcg gcggaagacg
      181 gccagcacca ggtctgccag cggcaccacc aggatcgaga tcggcagcag gatgggcagc
      241 aggctggccc gtgcgtccac gttgcccagg tcgtcggccg agaactggcc ggtgatcgtg
      301 atggtggacg ccgcgagcac caggccgatc agcatcgacc cggagtcgcc catgaagagg
      361 cgggcgggat ggaagttgtg gggcaggaag ccggtgcagg ccccggccag cgccgcgctc
      421 agcagggcgc cggtggtggc cagggtgacg tcgttgcggg cggtgaggac gtagcagaag
      481 atgaagaagg cgacggcccc cacgccgacg acgcccgccg ccagcccgtc gaggccgtcg
      541 acgaagttca cggcgttcac ggtggcgacc acgatgaaca ccgtcagcag ggcgccctgc
      601 gtcgggtcga tcgtcagcgc gccgccgtcg ggcatgggca cgtagaagaa ctgcacgccc
      661 tgccagacca agatccccgc cgcgagcacc tggccgccga acttggtgag ggcatcgagg
      721 tcgaagatgt cgtcgagcac ccccacggtg cagatcaccg cgcccgcgat caggacggcc
      781 cgggcctggt cgaagatgta ggggtcggcg cgggagagga acggcagctc gcggccgatc
      841 aggtaggcag ccaccagccc gcccaacatc gccaggccgc cgaggtaggg gatcggctcg
      901 tcgtggacgt cgcggtctcg cacgacggcg aaggccttcg tccgcagagc gaactcgcgg
      961 gcgacgacgg tgaggaggta cgtcgcggcg gcggcgacca ggaagacgag gatgtactcc
     1021 ctcacggcgc ggagtcagcc ctcgtcgagg aggaggccgg cccccgcgga ctcgaggacg
     1081 gcgttgagct cctcgaaggc gagcgcaccg cggcgcagga tccgaggccg gacgcccgtc
     1141 acgtcgagga tcgtggagcc gacggcgtcg ccgcctcgag gtgagtcccc cgcatcgaga
     1201 atgacggaga cgcggccccc cagcatctcc tcggccatga cggcgtcggt cgcggcgggc
     1261 ctgccggtga agttggccga ggagaccgcc agcgggccgg tccgctcgag cagctcgagg
     1321 gcgatcggat ggtcgggcat ccggatcgcc accgtgccgc gggtgtcgcc gaggtcccac
     1381 tgcagcgacg gctgttgctt cccgaccagc gtcagcgggc ccggccagaa gtgctccatc
     1441 agcgtgcgcg cgtacccagg cagagcgagc accagcgcgt cggccgtggt ggcggagctg
     1501 atcagcaccg gcggcggcat gtcacgacct cggcccttgg cctccagcag ggcggcgacg
     1561 gcatcctcgt cgaacgcgtc ggcgccgacg ccgtagacgg tgtcggtggg gagcaccacc
     1621 agtccaccgc gccggatggc gagcgcggcc gcctcgatgg cggcttcgcg tt
//
LOCUS       DASWNJ010000365         6029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_677118, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000365 DASWNJ010000000
VERSION     DASWNJ010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677118"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3184
                     /gene="dnaE"
                     /locus_tag="VGB85_06375"
     CDS             <1..3184
                     /gene="dnaE"
                     /locus_tag="VGB85_06375"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY0133684.1"
                     /translation="AGDMDAARTTVQEYQSIFEPGMYFLELQPNLMEAQEKVNAALAQ
                     LGADLGAPLVATNDCHYVNKDEAHAHEVLMCMGQGRTLDDPKRMKHDCDAFYIKRADE
                     MAPYFRQYPGAYENAGRIAAMCNVELKLGKPELPDFKLPEGTQEDLPGYLRKVAFEGL
                     ETRFHELTKRGQRFDPDLYRERLRFELGVIEQMKFPGYFLIVWDFIRHAKNIGVPVGP
                     GRGSGAGSLVAYALRITDLDPIQYSLLFERFLNPERVSMPDFDIDFCMDRREEVLQYV
                     TDRYGVDHVAQIATFHSLKARGLVRDVCRVMGLQPSEANEIAKAVPEGPKVTLSMALA
                     DPVALKDKVKKNPELAAKYQDAIMVAEGSGKLRQRARDDAKVAEVLKIGTALEGLNRH
                     AGMHAAGIVISNGKLWEHVPCFQADGKIVTQYTMNDVELAGLVKFDFLGLKTLTVLST
                     AVGLINEVRAVDKQEPFDLAEIPMDDAGVYEMISRGDTTGVFQLESSGFKDLLKKLRP
                     DTFGDIVAAVALYRPGPLEGGMVDQFIDCKHGRKQIEYPHPLLEDVLKETYGVFVYQE
                     QVMQAAQVLAGFSLGSADMMRRAMGKKKQSEMDKQRKLFVDGCASASNIPHEKASEIF
                     DLIDKFAGYGFNKSHSAAYGLITYQTAYLKHHHEASFMAALMTCDKANNDNVVKFIAE
                     ARAMGLTVLSPDVNESGRDFSVVRRRKDDGSHEEQIRFGLAGVRGVGENAVDAILNAR
                     KSGGPFVSLFELCRRVDLKKVNKRTLEGLTCAGAFDLVAGGRDRAAVLGAIESAVEQG
                     QGASRDRDSGQKGLFDMFAAQTPVYVENYPTGEAWAPKIKLLKEREALGFYLTGHPLD
                     RFQGDIDAGGARRTGQLTREQQGQEVTLAGVVCDLREVQTRSGKGSMGFFQLEDQFGR
                     VECVVFPKTYARLDEQTGLSFSDRLQSIGDEPVFVTGKLEVETDDEGEANKFKLLVDE
                     ARPIADVRAERTKTVMLKLTLEQLTDDRILRLKQIVADHGGPCTMELRVVSESFGADI
                     VFGDQFSVRADDNLVHALERLFGEKVAYLV"
     gene            3321..5099
                     /locus_tag="VGB85_06380"
     CDS             3321..5099
                     /locus_tag="VGB85_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133685.1"
                     /translation="MRTWKLGWGLVLGLAACGDNGGGETGSAGTGTSTTDATGTTPTT
                     GGETTQSPGDPGGTTDTGPDSISISITDTGPETDTGDPPSGFRCLAPPNDAVADLPAV
                     QIDTAYPALTGQTLHVAAGGDLQAAIDAAAPGDEITLAAGASFTGNFVLPEKAGDGWI
                     VIRSDAAGLPPEHTRVSLDDAVHMATLVTPSDLSVVSTAGAAHHFRFVGIEFRPADGV
                     SPNDLIVLGDGGATSVDQLAHDLVFDRCIVRGDPATGGKRGIQLNARSVGVVQSYFTD
                     WKREGQDTQTMLGWNTPGPFRIVDNHLEGAGENIMFGGADAKIADVIPSDIEICGNHF
                     TKPLRWKVDDPGYEGTHWSVKNLFELKVGRRVLISGNTFEQVWADAQTGFAIVLKSAN
                     QDGGQPWAVTEHVNFVYNEIRMANNGIAVSRTDGGSLGTNHVRIAHNVITDIGAATWG
                     GGGRIFQLLDGVADVKVVHNTGFGISHAIIFDGAPLPALAIEDNILGPTTYGVFGSGQ
                     GEGSGALGFYAPGGSLVGNVIVGASMASYPAGNSFPATVEEVGFVDLAGGNLGLVPGS
                     TYAGAASDGTDPGASIELVAQAKSGQ"
     gene            complement(5109..5957)
                     /locus_tag="VGB85_06385"
     CDS             complement(5109..5957)
                     /locus_tag="VGB85_06385"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEY0133686.1"
                     /translation="MQRRDLLRIVPALGVLACTEEPVMPAAATSTRPPVLFIAHGAPM
                     LLDEPGWVAELGRWSAALPRPRAILMISAHWEARPLTIGATSPRPLIYDFPGFPERFY
                     RLTYASPGAPLLADRVRDLLRGAGLKFSEAPTRGLDHGSYIPLICMYPEADVPVLQIS
                     MPSLDPAELLAMGRALAPLRDEGVLIAGSGFLTHNLRAMRSGATPTWAAEFDAWTADT
                     LASRDIDALLDYRARAPGVALSHPTHEHFAPVIVAAGAAIDDPSTFPIDGWWYGSFTR
                     RSVQFG"
BASE COUNT         1041 a         1979 c         2168 g          841 t
ORIGIN      
        1 cgcgggggac atggacgccg cgcgcacgac ggtccaggag tatcagtcga tcttcgagcc
       61 gggcatgtac ttcctcgagc tgcagcccaa cttgatggag gcccaggaga aggtcaacgc
      121 cgcgctcgcg cagctcgggg cggacctcgg ggcgccgctg gtcgccacca acgactgcca
      181 ctacgtcaac aaggacgagg cccacgcgca cgaggtgctg atgtgcatgg ggcaggggcg
      241 cacgctcgac gaccccaagc ggatgaagca cgactgcgac gcgttctaca tcaagcgcgc
      301 cgacgagatg gcgccgtact ttcgccagta tccgggcgcc tacgagaacg ccggtcggat
      361 cgcggcgatg tgcaacgtcg agctcaagct cggcaagccc gagctgccgg acttcaagct
      421 gcccgagggc acgcaggagg acctgccggg ctacctgcgc aaggtcgcgt tcgaggggct
      481 cgagacccgc ttccacgagc tgacgaagcg aggccagcgc ttcgacccgg acctgtaccg
      541 cgagcgcctg cgcttcgagc tcggcgtcat cgagcagatg aagttcccgg gctacttcct
      601 gatcgtgtgg gacttcatcc ggcacgccaa gaacatcggc gtgccggtcg gcccgggtcg
      661 tggatccggc gccggcagcc tcgtcgcgta cgcgctgcgg atcaccgacc tcgacccgat
      721 ccagtacagc ctgctgttcg agcgcttcct caaccccgag cgcgtgtcga tgcccgactt
      781 cgacatcgac ttttgcatgg accgccgcga ggaggtgctc cagtacgtga cggaccgcta
      841 cggcgtcgat cacgtggccc agatcgccac tttccatagc ttaaaggccc gggggctggt
      901 gcgggacgtg tgccgcgtga tggggctgca gccgtccgag gccaacgaga tcgccaaggc
      961 ggtgcccgag gggccgaagg tgacgctgtc gatggcgctc gccgatccgg tcgcgctcaa
     1021 ggacaaggtc aagaagaacc ccgagctggc cgcgaagtac caggacgcga tcatggtcgc
     1081 ggagggcagt ggcaagctga ggcagcgcgc gcgcgacgac gccaaggtgg ccgaggtgct
     1141 caagatcggc accgcgctcg agggcctcaa ccgccacgcg ggcatgcacg cggccggcat
     1201 cgtgatcagc aacggcaagc tgtgggagca cgtgccctgc ttccaggccg acggcaagat
     1261 cgtcacgcag tacacgatga acgacgtcga gctcgccggc ctggtgaagt tcgacttcct
     1321 cggcctcaag acgctgaccg tgctgtcgac cgcggtcggc ctgatcaacg aagtgcgcgc
     1381 tgtcgacaag caggagccgt tcgacctcgc cgagatcccg atggacgacg cgggcgtcta
     1441 cgagatgatc agccgcggcg acacgaccgg cgtgttccag ctcgagtcga gcggcttcaa
     1501 ggacctgctc aaaaagctgc ggcccgacac gtttggggac atcgtcgctg cggtcgcgct
     1561 gtaccggccg ggtccgctcg agggcggcat ggtcgaccag ttcatcgact gcaagcacgg
     1621 ccgcaagcag atcgagtacc cgcacccgct gctcgaggac gtgctcaagg agacgtacgg
     1681 cgtgttcgtg taccaggagc aggtgatgca ggcggcccag gtgctcgcag gcttcagcct
     1741 cggatccgcg gacatgatgc gccgcgcgat gggcaagaag aagcagtccg agatggacaa
     1801 gcagcgcaag ctgttcgtcg acggctgcgc gagcgcctcg aacatcccgc acgagaaggc
     1861 gagcgagatc ttcgacctga tcgacaagtt cgccggctac ggttttaata aatcgcactc
     1921 ggcggcgtac ggcttgatca cgtaccagac cgcgtacctc aagcatcatc acgaggcgtc
     1981 gttcatggcg gcgctgatga cctgcgacaa ggcgaacaac gacaacgtcg tcaagttcat
     2041 cgccgaggcg cgcgcgatgg gcctgacggt gctctcgccg gacgtcaacg agtcgggccg
     2101 cgacttctcg gtggtgcgcc ggcgcaagga cgacggctcg cacgaggagc agatccgctt
     2161 cggcctcgcg ggtgtgcgcg gcgtcggcga aaacgcggtc gacgcgatcc tcaacgcgcg
     2221 caagtccggc ggcccgttcg tcagcctgtt cgagctgtgc cgccgcgtcg acctcaagaa
     2281 ggtcaacaag cgcacgctcg agggtctcac ctgcgccggc gcgttcgacc tggtcgcggg
     2341 cggacgtgac cgcgcggccg tgctcggggc gatcgagtcg gcggtcgagc agggccaggg
     2401 cgcctcgcgg gaccgcgaca gcgggcaaaa aggcctgttc gacatgttcg ccgcgcagac
     2461 gccggtgtac gtcgagaact acccgaccgg cgaggcgtgg gccccgaaga tcaagctgct
     2521 caaggagcgc gaggcgctcg gcttctattt gaccgggcac ccgctcgacc gctttcaggg
     2581 ggacatcgac gccggcgggg cgcgccgcac cgggcagctg acacgcgagc agcagggcca
     2641 ggaagtgacc ctcgccggcg tcgtctgcga tctgcgcgag gtccagacgc gcagcggcaa
     2701 ggggtcgatg ggcttcttcc agctcgagga tcagttcggc cgcgtcgagt gcgtggtgtt
     2761 cccgaagacc tacgcgcgcc tcgacgagca gacgggcctg agcttcagcg accgattaca
     2821 gagcatcggc gacgagccgg tgttcgtcac cggcaagctc gaggtcgaga ccgacgacga
     2881 gggcgaggcc aacaagttca agctgctcgt cgacgaggcg cgcccgatcg cggatgtccg
     2941 cgccgaacgc accaagacgg tcatgctcaa gctgacgctc gagcagctga ccgacgaccg
     3001 gatcctgcgg ctcaagcaga tcgtcgcgga ccacggcgga ccctgcacga tggagctgcg
     3061 ggtcgtcagc gagagttttg gcgcggatat cgtgttcggc gaccagttca gcgtgcgcgc
     3121 ggacgacaac ctcgtgcatg cgctcgagcg gctgttcggc gagaaggtcg cttacctggt
     3181 ctgatcgggg ccgcgcgtgc tcggggacgg gcacgcgcat gcgcatgcgc gatggtgagg
     3241 ccgtcgcggt gacgcgaacg caggcatgtg gcctggcgcc ccttgtgcgc cgcggcctca
     3301 ccgagaaacg atccccggcg atgcgaacat ggaagctggg atgggggctg gtgctcgggc
     3361 tcgccgcgtg cggcgacaac ggcggcgggg agacgggcag cgccggcacg gggacgagca
     3421 cgacggatgc gaccgggacg acgccgacca ccggcggtga aacgacgcag agcccgggcg
     3481 acccgggtgg gaccaccgac accggacccg actcgatctc gatctcgatc acggacaccg
     3541 ggcccgagac cgacaccggt gacccgccgt cgggctttcg ctgtctcgcg ccgccgaacg
     3601 acgcggtcgc cgacctgccg gccgttcaga tcgacacggc ctatccggcg ctgaccgggc
     3661 agacgctcca cgtggcggcc ggcggtgacc tgcaggccgc gatcgacgcg gcggcgcccg
     3721 gcgacgagat cacgctcgcg gcgggcgcga gcttcaccgg gaatttcgtg ctgcccgaga
     3781 aggccggcga cggctggatc gtgatccgca gcgatgccgc cggtttgccg cccgagcaca
     3841 cgcgcgtcag cctcgacgac gcggtgcaca tggcgacgct ggtgacgccg agcgacctgt
     3901 cggtggtcag cacggccggc gcggcgcacc actttcggtt cgtcgggatc gagtttcgcc
     3961 cggccgatgg ggtcagcccc aacgacctga tcgtgctcgg cgacgggggg gcgacctcgg
     4021 tcgaccagct cgcgcatgac ctcgtgttcg atcgctgcat cgtccgcggc gatccggcga
     4081 ccggcggcaa gcgtgggatc cagctcaacg cgcggtccgt cggcgtcgtc cagtcgtact
     4141 tcaccgactg gaagcgcgag gggcaggaca cgcagacgat gctcggctgg aacacgccgg
     4201 ggccgtttcg catcgtcgac aaccacctcg agggcgccgg cgagaacatc atgttcggcg
     4261 gggccgacgc gaagatcgcc gatgtcatcc ccagcgacat cgagatctgc ggcaaccact
     4321 tcaccaagcc gctgcgctgg aaggtcgacg accccggcta cgagggtacc cactggtcgg
     4381 tcaagaacct gttcgagctc aaggtcgggc gccgcgtgct gatctccggc aacaccttcg
     4441 agcaggtctg ggccgacgcg cagacggggt ttgcgatcgt cctcaagtcg gcgaaccagg
     4501 acggcgggca gccgtgggcg gtcaccgagc acgtcaactt cgtctacaac gagatccgca
     4561 tggccaacaa cggcatcgcc gtgtcgcgca cggacggcgg atcactcggc accaaccacg
     4621 tgcggatcgc ccacaacgtg atcaccgaca tcggcgccgc gacctggggc ggtggtggcc
     4681 ggatcttcca gctgctcgac ggcgtcgccg acgtgaaggt cgtccacaac accggcttcg
     4741 ggatcagcca cgcgatcatc ttcgacggtg cgccgctgcc ggcgctggcg atcgaggaca
     4801 acatcctcgg gccgacgacg tacggcgtgt tcggcagcgg acagggcgag ggcagcggcg
     4861 cgctcggctt ctatgcgccc ggcggctcgc tggtcggcaa cgtgatcgtc ggcgccagca
     4921 tggcgagcta tcccgcgggc aacagcttcc cggcgaccgt cgaggaggtc ggcttcgtcg
     4981 atctcgcggg cgggaacctc ggcctcgtgc caggctcgac ctacgccgga gcggcgagcg
     5041 acggcaccga cccgggggcg tcgatcgagc tcgtcgcaca ggcgaagagc gggcagtgac
     5101 ggatattttt acccgaactg caccgagcgc cgcgtgaacg acccgtacca ccagccgtcg
     5161 atcgggaagg tcgagggatc gtcgatcgca gcgccggccg cgacgatcac cggcgcgaag
     5221 tgttcgtggg tcggatgcga gagggcgacg ccgggggcgc gggcgcggta gtcgagcagg
     5281 gcgtcgatgt cgcgagacgc gagggtgtcg gcggtccagg cgtcgaactc ggcggcccag
     5341 gtcggcgtgg cgccgctgcg catggcccgg aggttgtggg tgaggaagcc gctgcccgcg
     5401 atcagcacgc cctcgtcgcg cagcggcgcg agggcgcggc ccatggcgag cagctcggcg
     5461 gggtcgagcg agggcatcga gatctgcagc accgggacgt cggcctccgg gtacatgcag
     5521 atcaacggga tgtagctgcc gtgatcgagg ccgcgggtcg gcgcctcgga gaacttcagg
     5581 cccgcgccgc gcagcaggtc acgcacgcga tctgccagga gcggcgcgcc gggggacgcg
     5641 taagtcaggc gatagaagcg ctcggggaag ccggggaagt cgtagatcag cggtcgcggg
     5701 ctggtcgcgc cgatcgtcag cgggcgggcc tcccagtgcg ccgagatcat caggatcgcg
     5761 cgcggacgcg gcagggcggc cgaccagcgg ccgagctcgg cgacccagcc gggctcgtcg
     5821 agcagcatcg gcgcgccgtg ggcgatgaac agtacgggcg gtcgggtcga cgtggctgcg
     5881 gcgggcatga cgggctcctc ggtgcacgcg aggaccccga gcgccggtac gatccgaagc
     5941 aggtctcggc gttgcatcga gatggtctac gcacccgcgc gggcctcaac aagcggcccg
     6001 agtgctgcga gcgcgacctc ggcttcgac
//
LOCUS       DASWNJ010000366         1727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_537631, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000366 DASWNJ010000000
VERSION     DASWNJ010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537631"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1727)
                     /locus_tag="VGB85_06390"
     CDS             complement(<1..>1727)
                     /locus_tag="VGB85_06390"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0133687.1"
                     /translation="GGTPVTVGDLGTVRTGPELRRGLAELDGEGEVVGAIVIARYDTH
                     ALDVIQRVKARLATVAAGLPPGVEVVPTYDRSALIHRAVDTLQHALIEEGIVVALVII
                     LFLLHLRSALLPILSLPLAVLLAFIPMAWLGIPATIMSLGGIAIAVGATVDAEIVMIE
                     ACHKRLEHAPPGISGAARRKLLAEAAREVTPAIFFSLLIIAVAFIPVFGLEGQAGRLF
                     KPLAYTKTFVMLAAALLSVTFAPALRDLLIRGKIRKEQDHPISRLIRRVYEPFVYVAL
                     RRPITTLMIGGLAVLSAIPVTMRLGSEFMPALNEGDILYMPTTLPGLSIEEAKRQLAR
                     QDAILAAFPEVSRVFGKVGRAETPTDPAPLSMVETVVMLRPPNEWPTVETPRWHSGWA
                     PRWLGLRHLWPEARPESWDELVAKLGASLQLPGWTGAYTMPIRARIDMLTTGVRTPVG
                     VKVFGADLEAIERTGLALESLLRTVPGTRSILYERSLGGVYVDVIPRRDALARHGLAP
                     EDLTALVEDAVGGETITTTVEGRRRFSVQVRLVEDARSSPERLRELPVPLPGPPGPAG
                     IARAVPLGE"
BASE COUNT          253 a          552 c          709 g          213 t
ORIGIN      
        1 ctcgccgagc ggcacggcgc gcgcgatccc ggcggggccg ggcggacccg gcaggggcac
       61 cggcaactcg cgcaggcgct cggggctcga gcgggcgtcc tccacgaggc gcacctgcac
      121 ggagaagcgg cgccggccct cgacggtggt ggtgatcgtc tcgccgccga cggcgtcctc
      181 gacgagcgcg gtgagatctt caggggccag gccgtggcgg gcgagcgcgt cgcgccgggg
      241 gatcacgtcg acgtagacgc cgccgaggct gcgctcgtag aggatcgatc gcgtgcccgg
      301 gaccgtgcgc agcaggctct cgagcgcgag cccggtgcgc tcgatcgcct cgaggtcggc
      361 gccgaagacc ttgacgccga cgggcgtgcg cacgccggtg gtcagcatgt cgatgcgcgc
      421 ccggatcggc atggtgtacg cgccggtcca gccgggcagc tggaggctcg cgccgagctt
      481 ggcgacgagc tcgtcccacg actccgggcg agcctcgggc caaaggtgac gcaggccgag
      541 ccagcgcggg gcccagccgc tgtgccagcg cggggtctcg acggtcggcc actcgttcgg
      601 cgggcgcagc atgacgacgg tctcgaccat gctcagcggc gcggggtcgg tcggggtctc
      661 ggcccggccg accttgccga acacgcggct gacctcgggg aaggcggcga ggatcgcgtc
      721 ctggcgcgcg agctgccgct tggcctcctc gatcgagagg ccgggcaggg tggtcggcat
      781 gtagaggatg tcgccctcgt tgagggcggg catgaactcg ctgccgaggc gcatcgtcac
      841 cgggatcgcg gacagcacgg cgaggccgcc gatcatcagg gtggtgatag ggcggcgcag
      901 ggcgacgtag acgaagggct cgtagacgcg gcggatcagc cgcgagatcg ggtggtcctg
      961 ctccttgcga atcttgccgc ggatcagcag gtcgcgcagc gccggggcga aggtgacgct
     1021 cagcagcgcc gcggcgagca tgacgaaggt cttggtgtag gcgagcggct tgaacaggcg
     1081 cccggcctgg ccctcgaggc cgaacacggg gatgaaggcg acggcgatga tcagcagcga
     1141 gaagaagatc gcgggggtga cctcgcgggc ggcctcggcg agcagcttgc ggcgggccgc
     1201 gcccgagatg ccgggcgggg cgtgctcgag gcgcttgtga caggcctcga tcatgacgat
     1261 ctcggcgtcg acggtggcgc cgacggcgat cgcgatcccg ccgaggctca tgatcgtcgc
     1321 cgggatcccg agccaggcca tcgggatgaa ggcgagcagc accgcgagcg ggaggctgag
     1381 gatcggcaag agggcgctgc gcaggtgcag caggaacagg atgatgacga gggcgacgac
     1441 gatgccctcc tcgatcaagg cgtgctgcag ggtgtcgacg gcgcggtgga tcagggcgga
     1501 gcggtcgtag gtcggcacga cctcgacgcc cggcggcaag ccagcggcca cggtggcgag
     1561 gcgggccttg acgcgctgga tgacgtcgag cgcgtgggtg tcgtagcggg cgatgacgat
     1621 cgcgccgacg acctcgccct cgccgtcgag ctcggcgagg ccgcggcgca gctcggggcc
     1681 ggtgcgcacg gtgccgaggt caccgacggt caccggggtg ccgccgg
//
LOCUS       DASWNJ010000367         4819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_677119, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000367 DASWNJ010000000
VERSION     DASWNJ010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677119"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1443)
                     /locus_tag="VGB85_06395"
     CDS             complement(19..1443)
                     /locus_tag="VGB85_06395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEY0133688.1"
                     /translation="MHRMTGMDASFLYMESPRLPMHTLKLVIVDVADVPGGYRFERVA
                     EVVAAHLHLLPALRRRALAVPLGLHHPVWIDDPAFRIGDHIERVVLPEGAGDAELDAF
                     TASVTEGPLDRRRPLWHMWAIEGMRGAEGRPLVAFLVKIHHAIADGVAAEEMLRLAMS
                     ELDGSTPLGSGPTPLPDRVALALGALRDHLRQRARLPRLLLGTLRGGLRAMWLTRRAE
                     VKAPRLFSAPRTVFNHKISSRRNFARVELPLPEFRRIKAVFACTINDVVLAVVAGALR
                     AYLLGRGEAAEFPLIASVPACTDHEIDNPTAGNHVSSLYTSLRVDLADPVERLEATRQ
                     VTDAAKAKHAAMGSHMLTEWMEFSRLAPHAWLWRRLLPRLARPPLHLVVSNVPGPREP
                     LAIDGARLVRLASVGPLLEGVGLNITVWSYIDTMSFAVLASPDGIPELAPLIELLRTS
                     LAELGAAADRRVEAEAQVPVAEAG"
     gene            complement(1473..2783)
                     /locus_tag="VGB85_06400"
     CDS             complement(1473..2783)
                     /locus_tag="VGB85_06400"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0133689.1"
                     /translation="MSWAILIVGVTGVLSTLNAFFPVRTRWLAILGFFAGWLTMELTL
                     HILVGTAVLLGVLIAYGGLQAWPGQLGLALAAASAGGLLGLYLRSRNARVTLREFYKS
                     AELQVGPDAPRYPRSHIVFPFLAFHRRDIEVVRNIPFSQVAGQTLRLDVFRPKGPSKG
                     LRRPAVLQIHGGAWVLGFKEYQGIPLLSHLATQGWVGFNIDYRLSPKASFPEHLIDCK
                     QALAWIREHADEYGIDPDFIVVTGGSAGGHLAALVGLTAGDPEYQPGFESADTSVHAA
                     VVFYGVFDLTNRLGTRIGGYRAMLERVVMKAKYDERPELFSRASPIDRVHPGAPTFCV
                     IHGTRDTMTPVEDARYFVEKLRAVSSKPALYIEMQGAEHAFDTFPSIRTVPVIEAIER
                     FLDSLYSAYCAGKGAAHGAARTIPAITDVASTSTPANAYPAAAP"
     gene            complement(2945..>4819)
                     /locus_tag="VGB85_06405"
     CDS             complement(2945..>4819)
                     /locus_tag="VGB85_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEY0133690.1"
                     /translation="GDLGPDEQCDDGADNGDTNSCTADCQNNVCGDGKVGPGEGCDDG
                     NQVEDDECSNMCVPSTCGDGKVAPTEACDDGTANNTDECPDSCPAAACSDGFIQPSAG
                     ESCDDGLDNANNAACTTECQANVCGDGNVYNTGAGDEACDDGPDNGPGKSCNADCQLN
                     VCGDGDMGPDEQCDDGNGDPGDGCSATCVLEGCGNNIVDPGETCDDGKNGDNDDDCTD
                     ECQAPACGDGFEQPGQGEQCDLGGDNSDAGACTTTCKSAICGDSLIQQNVEQCDDGPN
                     NGPGKSCKADCQSNFCGDGDKGPGEACDDGNQSNDDACTNVCKTATCGDGFKQPGEAC
                     DLGVLNDNTGACTLACTVPVCGDGFVQPTAGETCDDGNMSSQDACLATCKPASCGDGQ
                     VWTGMEECDDGNDVNADACTNMCKAPTCNDNIKNGTETATDCGGPMCKPCSVAGLVIN
                     EVDYDQIGTDSAEFVEILNTTNAPINLTGHAIVLVNGADSKVYTTVNLAGAGTINAGQ
                     YLVVAPANFAVPNGVLKVSFALTTDQIQNGNPDGLALINTATMKLIDALSYEGSITAA
                     VIPNVGTVSLVEGMPFAAAQSNDFVDSHVRLPNGYDGNNAATDWVLSKNPTPGLPNVP
                     "
BASE COUNT          665 a         1665 c         1738 g          751 t
ORIGIN      
        1 gtccgcgagg gctgagccct accccgcctc ggcgaccggg acctgcgcct ccgcctcgac
       61 gcggcggtcc gcggcggcgc cgagctccgc gagcgacgtc cgcagcagct cgatcaacgg
      121 cgcgagctcc gggatgccgt cgggggacgc gagcaccgcg aagctcatcg tgtcgatgta
      181 gctccacacg gtgatgttga ggccgacgcc ctcgagcagc gggccgaccg acgccaggcg
      241 cacgagccgg gccccgtcga tcgccagcgg ctcgcgtggg ccgggcacgt tcgagacgac
      301 gaggtgcagc ggcggacggg cgagccgcgg caacaggcgc cgccacagcc acgcgtgcgg
      361 ggcgaggcgc gagaactcca tccactccgt cagcatgtgc gagcccatcg ccgcgtgttt
      421 ggccttggcg gcgtcggtca cctgccgggt cgcctcgagg cgctcgaccg ggtcggcgag
      481 gtcgacgcgc agcgaggtgt agaggctcga cacatggttg cccgcggtcg ggttgtcgat
      541 ctcatggtcg gtgcacgcgg gcaccgaggc gatcagcggg aactcggccg cctcgccgcg
      601 cccgagcagg tacgcgcgca gggccccggc gacgaccgcg agcacgacgt cgttgatcgt
      661 gcacgcgaac accgccttga tccggcgaaa ctccggcagc ggtagctcga cgcgggcgaa
      721 gttgcgacgc gacgagatct tgtggttgaa caccgtgcgc ggggcggaga acaggcgcgg
      781 cgccttgacc tcggcgcgcc gcgtcagcca catcgcccgc agccccccgc gcagggtccc
      841 cagcagcagc cggggcaggc gcgcgcgctg gcgcaggtga tcccgcagcg cgccgagggc
      901 cagcgccacg cggtccggga gcggcgtcgg gccggatccc agtggcgtcg agccgtcgag
      961 ctcgctcatc gccaaacgca gcatctcctc ggcggcgacg ccgtcggcga tcgcgtggtg
     1021 gatcttgacc aggaacgcga cgagcgggcg gccctcggcg ccgcgcatgc cctcgatcgc
     1081 ccacatgtgc caaagcggcc gcctgcggtc gagcgggccc tcggtcacgc tcgcggtgaa
     1141 ggcgtcgagc tcggcatcac cggcgccctc cggcaagacc acgcgctcga tgtgatcgcc
     1201 gatccgaaac gccgggtcat cgatccatac cggatgatgc agcccgagcg gcaccgcgag
     1261 cgcccgccgg cgcagcgccg gtaacaaatg caggtgcgcc gcgacgacct cggcgacgcg
     1321 ctcgaagcgg taaccaccgg ggacgtccgc gacgtcgacg atcaccagct tgagcgtgtg
     1381 catcggcaga cgcggagact ccatgtataa aaaactcgca tccattccgg tcatgcgatg
     1441 catcgagagg ctcctttgtt tcgacgggat gctcagggag ccgccgcggg gtatgcgttc
     1501 gcaggcgtgc tcgtggacgc gacatcggtg atcgcgggga tcgttcgggc agcgccgtgc
     1561 gcggcgccct tgccggcgca gtaggccgaa tacagcgagt cgaggaagcg ctcgatcgcc
     1621 tcgatgaccg gcaccgtgcg gatcgaaggg aaggtgtcga aggcgtgctc ggccccctgc
     1681 atctcgatgt acagcgccgg cttcgacgac acggcgcgca gcttctcgac gaagtagcgc
     1741 gcgtcctcga cgggcgtcat cgtgtcgcgc gtcccgtgga tcacgcaaaa ggtcggcgcg
     1801 cccgggtgca cgcggtcgat cggcgaggct cggctgaaca gctccgggcg ctcgtcgtac
     1861 ttcgccttca tcaccacgcg ctcgagcatc gcccggtagc cgccgatccg cgtgccgagc
     1921 cggttggtga ggtcgaagac gccgtagaac acgaccgccg cgtgcacgct ggtgtccgcc
     1981 gactcgaagc ccggctgata ctccgggtca cccgcggtga gcccgacgag cgcggcgagg
     2041 tggcccccgg ccgagccgcc ggtgacgacg atgaagtcgg ggtcgatgcc gtactcgtcg
     2101 gcgtgctcgc ggatccacgc gagcgcctgc ttgcagtcga tcaggtgctc ggggaacgag
     2161 gccttggggc tcaggcgata gtcgatgttg aagccgaccc agccctgggt cgccaggtgc
     2221 gacagcagcg ggatcccctg atactccttg aagcccagca cccaggcgcc gccgtggatc
     2281 tgcagcacgg cgggccggcg caggcccttg ctcgggccct tcgggcggaa cacgtcgagg
     2341 cgcagggtct ggccggcgac ctgcgagaac gggatgttgc ggacgacctc gatgtcgcgc
     2401 cggtggaagg ccaggaacgg gaacacgatg tgactgcgcg gatagcgggg cgcgtccgga
     2461 ccgacctgca gctcggccga cttgtagaac tcgcgcagcg tgacccgggc gttgcgcgag
     2521 cgcaggtaga gcccgagcag gccgcccgcg gaggcagcgg cgagcgccag gccgagctgc
     2581 ccaggccacg cctgcaggcc gccgtacgcg atcaggaccc cgagcagcac ggccgtgccg
     2641 acgaggatgt gcagggtcag ctccatcgtc agccagcccg cgaagaaccc gaggatcgcc
     2701 agccagcgcg tgcgtaccgg gaagaacgcg ttgagcgtgg agagcacgcc ggtaacaccg
     2761 acgatcagga tcgcccagga caccccgcga gtgtggcacg cgcccgctgc gcggcaaccg
     2821 cggccgggcc gacccgccgc cggactcggc ccgcggcggg ccggcgttcc ccgcgaagat
     2881 cgggggatcg ccggcccgag gactgcgctg ccggcgaaac ccgcggtccc gcgcccggga
     2941 accctcaggg cacgttgggc aggccgggcg tcgggttttt cgagagcacc cagtcggtcg
     3001 ccgcgttgtt gccgtcgtag ccgttgggca ggcggacgtg tgagtcgacg aagtcgttcg
     3061 actgcgccgc cgcgaagggc atgccctcga cgaggctgac ggtgccgacg ttcgggatca
     3121 cggccgcggt gatcgatccc tcgtaggaca gcgcgtcgat cagcttcatc gtcgcggtgt
     3181 tgatcagcgc gaggccgtcg gggttaccgt tctggatctg atccgtggtc agggcgaagc
     3241 tcaccttgag cacgccgttg ggcacggcga aattcgccgg ggcgacgacg aggtactgac
     3301 cggcgttgat cgtgccggca cccgcgaggt tgaccgtcgt gtagaccttg ctgtcggcgc
     3361 cgttgacgag cacgatcgcg tggccggtca ggttgatcgg cgcgttggtg gtgttgagga
     3421 tctcgacgaa ttcggcgctg tccgtgccga tctggtcgta gtcgacctcg ttgatcacca
     3481 gcccggcgac cgagcacggc ttgcacatcg gcccgccgca gtcggtcgcg gtctccgtgc
     3541 cgttcttgat gttgtcgttg caggtcgggg ccttgcacat gttggtgcag gcgtcggcgt
     3601 tgacgtcgtt gccgtcgtcg cactcctcca tgcccgtcca cacctgaccg tcgccgcagc
     3661 tcgccggctt gcaggtcgcc aggcacgcgt cctggctcga catgttgccg tcgtcgcagg
     3721 tctcgcccgc ggtcggctgc acgaagccgt cgccgcagac cggcacggtg cacgccagcg
     3781 tgcacgcgcc ggtgttgtcg ttgagcacgc cgaggtcgca ggcctcgccc ggctgcttga
     3841 agccgtcgcc gcaggtcgcc gtcttgcaca cgttggtgca cgcgtcgtcg ttgctctggt
     3901 tgccgtcgtc gcaggcctcg cccgggccct tgtcgccgtc accgcagaag ttgctctggc
     3961 agtcggcctt gcacgacttg ccgggcccgt tgttggggcc gtcgtcgcac tgctcgacgt
     4021 tctgctggat gaggctgtcg ccgcagatcg cggacttgca cgtggtggtg cacgcgccgg
     4081 cgtcgctgtt gtcgccgccg aggtcgcact gctcgccctg ccccggctgc tcgaagccgt
     4141 cgccgcacgc gggcgcctgg cactcgtcgg tgcagtcgtc gtcgttgtcg ccgttcttgc
     4201 cgtcgtcgca ggtctcgccg gggtcgacga tgttgttgcc gcaaccctcg agcacgcagg
     4261 tcgccgagca gccgtcgccg gggtcgccgt tgccgtcgtc gcactgctcg tcggggccca
     4321 tgtcgccgtc gccgcacacg ttgagctggc agtcggcgtt gcacgacttg cccgggccgt
     4381 tgtcggggcc gtcgtcgcag gcctcgtcgc cggcgccggt gttgtagacg ttgccgtcgc
     4441 cgcacacgtt cgcctggcac tcggtcgtgc acgcggcgtt gttggcgttg tcgaggccgt
     4501 cgtcgcaact ctcgccggcc gacggctgga tgaagccgtc gctgcacgcc gcggccgggc
     4561 agctatcggg gcactcgtcg gtgttgttcg cggtgccgtc gtcacacgcc tcggtcggcg
     4621 cgaccttgcc gtcgccgcag gtcgagggca cgcacatgtt cgaacactcg tcgtcctcga
     4681 cctggttgcc gtcgtcgcag ccctcgcccg gcccgacctt gccgtcgccg cagacgttgt
     4741 tctgacagtc ggcggtgcag ctgttcgtgt cgccgttgtc ggccccgtcg tcgcactgct
     4801 cgtcgggccc gaggtcgcc
//
LOCUS       DASWNJ010000368         3277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_430949, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000368 DASWNJ010000000
VERSION     DASWNJ010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430949"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(739..1281)
                     /locus_tag="VGB85_06410"
     CDS             complement(739..1281)
                     /locus_tag="VGB85_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133691.1"
                     /translation="MQHTTKFMILAAGALVLASGCARRQTDKMNAGEEDLYAKYQNTS
                     YEKDMVTAKETQEYEDQGQGVSPRVLKSIQDTVENVYERDFGRCLQRDMEEYENRWIA
                     GTFSVEFTIATSGKVTAARITAMDIKERKPPKGKKENQNREAKLFGPCVEESASKWEF
                     DPPPEVEYIYTYSGKVGERF"
     gene            complement(1335..2483)
                     /locus_tag="VGB85_06415"
     CDS             complement(1335..2483)
                     /locus_tag="VGB85_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0133692.1"
                     /translation="MDLSFTDDQQALLETVRQFATREVKPQAAALDREHRFPKELVAR
                     MAELGLMGIEVSDEYDGTGLDSIAYVLAMEEVSAACASTGVIMSVNNSLVCDPLVKFG
                     TEAQKQQWLRPLARGEKLGCFMLSEPNAGSDAAAQKTMAVRDGAGFVINGVKNWITNG
                     PQADTGILFTMTEPEKGNHGITAFVVDMNGEGVTRGPKDDKLGIRASHSCQIFFTDHR
                     VSQDQILGEPGKGFKVAMSTLDCGRIGIAAQALGIARAAFEAAAEYAGDRKTFGKRII
                     DHQAIGFMLADMLTEIDAARLLTLRAAWMKSRGQNHTRESAMAKLFASETANKVAKNA
                     LQVFGGNGYVTEYPAERHFRDAKITEIYEGTSEIQRLVIAASLLKSRK"
     gene            complement(2518..>3277)
                     /locus_tag="VGB85_06420"
     CDS             complement(2518..>3277)
                     /locus_tag="VGB85_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY0133693.1"
                     /translation="NRPQVLNALSVAVVHELTEAARDAGSDAAQGKIRGIVLTGAGAK
                     AFVAGADNAAMANMTEQEASEFARQGHALGDKLASLPIPVIAAVNGFALGGGCEVALA
                     CDFIYASEAARFGQPEVKLGVIPGFGGTQRLLRRVGLSRALELCMSGAIIDANEALRI
                     GLVNRVCKPEELLPAAIKTCETIAQMGPQAVAVAKEVLHRGADLALHAANALEIDAFA
                     QLFNTGDQSEGMAAFLGKRAAAFTGRPGTGHYQS"
BASE COUNT          455 a         1164 c         1105 g          553 t
ORIGIN      
        1 gacgcgggtc ctcccagagc acgtgctcgc cgccccatct gtcgtgatga ccatgacact
       61 gtcacccagc gtcgatcgct cgaccgaggg gacgaggggg cgtctgcgcg cgagccctgg
      121 gtcctcccgc tgcttccccc gccgccggcc cctacccccc ggaccagcac cgtcggcgcc
      181 ccgcttgtcg gtgcgcaaac ctgttcgagg acccgggcgc tgccacgggg agcatctgtc
      241 gagacatcaa caacaggtcg gtgcgcaccg tcgatgtcgc ggcttcccga cggaaccagc
      301 ctcgggacgg gcgatgcacc gtccgtgtcg cgtacctgca gcagcgcggc cggaggagac
      361 ccgggcgcgc ggagcagttg ccccggtcga gcgacggtga ggcttcccgc gacaagcggg
      421 gcgcatacgg tgtccgcccg gggagcaggg gccgactggc gggggacgca gcgggaggac
      481 ccagggctcg cgcgcaggcg ccccaccgcc gcgcacgagc ggagagacag aagaggtgaa
      541 agagtcatgt ccttccggac aggtggggcg ccgagcacgt gctctgggag gacccgcgtc
      601 gcagggcccc gcagcccgtc ggcccctact ccccggccca ggtctcctcg ccccgcgtca
      661 cccgaacacg ccatcgggcc ccccgaacac gccatgcact ccgaacgcgc catcgggccc
      721 cccgagcacg ccatcaatct agaaacgctc gccgaccttg ccggagtacg tgtagatgta
      781 ctcgacctcg ggcggcggat cgaactccca cttgctggcg gactcctcga cgcacggtcc
      841 gaacagcttg gcctcgcggt tctggttctc cttcttgccc ttgggcggct tgcgctcctt
      901 gatgtccatc gcggtgatgc gggccgcggt gaccttgccc gaggtagcga tcgtgaactc
      961 gaccgagaac gtgccggcga tccagcggtt ctcgtactct tccatgtcgc gctggaggca
     1021 gcggccgaag tcacgctcgt agacgttctc gaccgtgtcc tggatcgact tcaggacgcg
     1081 gggcgacacg ccctgaccct ggtcctcgta ctcctgcgtc tccttggcgg tgaccatgtc
     1141 cttctcgtag gacgtgttct ggtacttggc gtacaggtcc tcctcgcccg cgttcatctt
     1201 gtcggtctgg cggcgggcgc agccggacgc gaggaccaac gcgccggcgg caaggatcat
     1261 gaacttggtg gtgtgctgca tgggggctcc gggggcgctg tgcgaggtgg gtgcgagatt
     1321 gtaccagcgt gcagtcactt gcgcgacttg agcagggacg ccgcgatgac caggcgctgg
     1381 atctcgctgg taccttcgta gatctcggtg atcttggcgt cccggaagtg gcgctcggcc
     1441 gggtactcgg tcacgtagcc gttgccgccg aacacctgca acgcgttctt ggcgaccttg
     1501 ttggccgtct ccgacgcgaa cagcttcgcc atcgccgact cgcgggtgtg gttctgaccg
     1561 cgcgacttca tccacgccgc gcgcagcgtc agcaggcggg ccgcgtcgat ctccgtcagc
     1621 atgtcggcga gcatgaagcc gatcgcctgg tgatcgatga tccgcttgcc gaaggtcttg
     1681 cggtcgccgg cgtactcggc ggcggcctcg aaggccgcgc gggcgatgcc cagcgcctgc
     1741 gccgcgatgc cgatccggcc gcagtcgagc gtgctcatcg caactttaaa acccttgccg
     1801 ggctcgccga ggatctgatc ctgcgacacg cggtggtccg taaaaaagat ctggcacgag
     1861 tgcgaggcgc ggatgccgag cttgtcgtcc ttgggcccgc gcgtgacgcc ctcgccgttc
     1921 atgtcgacga cgaacgccgt gatcccgtgg ttgcccttct cgggctcggt catcgtgaac
     1981 aggatccccg tgtccgcctg cgggccgttg gtgatccagt tcttgacccc gttgatcacg
     2041 aagccggcgc cgtcgcgcac cgccatcgtc ttctgcgccg ccgcgtcgct gcccgcgttg
     2101 ggctcgctga gcatgaagca gccgagcttc tcgccgcgcg ccaaggggcg cagccactgc
     2161 tgcttttgcg cctccgtgcc gaacttcacc agcgggtcgc agaccagcga gttgttgacg
     2221 ctcatgatca cgcccgtcga cgcgcaggcg gccgagacct cctccatcgc cagcacgtac
     2281 gcgatcgagt cgaggccggt gccgtcgtac tcgtccgaca cctcgatgcc catcaggccg
     2341 agctcggcca tccgcgcgac cagctccttc gggaagcggt gctcgcggtc gagggcggcc
     2401 gcctgcggct tgacctcgcg ggtcgcaaac tggcgcacgg tctcgagcag ggcttgttgg
     2461 tcgtcggtga agctcagatc catcgtgtcc tcgtatctgt ctctcgggac ctgtccctca
     2521 ggactggtag tggcccgtac cgggtcgccc ggtgaacgcc gcggcgcgct tgccgaggaa
     2581 cgccgccatg ccctccgact ggtcgccggt gttgaacagc tgcgcgaagg cgtcgatctc
     2641 gagcgcgttg gcggcgtgca gcgccagatc ggcgccgcgg tgcagcacct ccttggcgac
     2701 cgcgaccgcc tgcgggccca tctgcgcgat cgtctcgcag gtcttgatcg cggccggcag
     2761 cagctcctcg ggcttgcaca cgcggttgac caggccgatc cgcagcgcct cgttcgcgtc
     2821 gatgatcgcc ccgctcatgc acagctcgag cgcgcggctg aggccgacgc gtcgcagcag
     2881 ccgctgggtc ccgccaaagc ccgggatcac gccgagcttg acctccggct ggccgaagcg
     2941 cgccgcctcg ctggcgtaga tgaagtcgca ggcgagcgcg acctcgcagc cgccgccgag
     3001 cgcgaagccg ttgaccgccg cgatcaccgg gatcggcagc gaggcgagct tgtcgccgag
     3061 cgcgtggccc tggcgggcga actcgctggc ctcctgctcg gtcatgttcg ccatcgccgc
     3121 gttgtcggcc ccggcgacga aggccttggc ccccgcgccg gtcagcacga tgccgcgaat
     3181 tttgccctgc gccgcgtcgc tgccggcgtc gcgcgcggcc tcggtcagct cgtgcacgac
     3241 cgccaccgac agcgcgttga gcacctgcgg gcggttg
//
LOCUS       DASWNJ010000369         1533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_607256, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000369 DASWNJ010000000
VERSION     DASWNJ010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607256"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VGB85_06425"
     CDS             complement(<1..463)
                     /locus_tag="VGB85_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133694.1"
                     /translation="MLRVRLAVVVAALLLATPARAHEFRAAVLLVDIGPDGVVELRLE
                     LPPGAQRPRLEFPAGCEVVRAEPLRATCGADGLRGELLVHALAPDLELVTHVRQDGQP
                     ARTQVLRHDSPALPLTPAGTGRAGYLRLGALHILGGPDHLLFVVGLALWVRG"
     gene            complement(463..1317)
                     /locus_tag="VGB85_06430"
     CDS             complement(463..1317)
                     /locus_tag="VGB85_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0133695.1"
                     /translation="MPRRVLSEPLLHFAALGLVFFGLHAALAPARGEALVVDAARVRA
                     TANELGRRLGRAPAEAELASALQTELDEERLYREALALGLERDDPIVRRRLIQKLRFV
                     HEDLADVAAPDDAALLAVRDADPGRYTTPARFALTQVFVARERHTDAEAVARALQRRL
                     LAGEDPGGLGDLCVHGQRFGARTAAAYAGVFGERFAAGLLDMSPGTWSIAQSDLGWHV
                     VRVDEMTPPALAPLAAVRPRLRADWEAARREASSEAALAGLRERYPVSFEGDVPPAVA
                     SALVERGP"
BASE COUNT          218 a          615 c          531 g          169 t
ORIGIN      
        1 cgccgcggac ccacaacgcg agcccgacga cgaacagcag gtgatccggc cccccgagga
       61 tatgcagcgc gccgagcctc aggtacccgg cccgcccggt cccggccggc gtcagcggca
      121 gcgcagggct gtcgtgccgc agcacctgcg tgcgcgccgg ctggccgtcc tggcgcacgt
      181 gcgtgacgag ctcgaggtcg ggcgcgagcg cgtgcacgag caattcacca cgcaacccgt
      241 cggcgccgca ggtcgcccgc aacggctcgg cgcgcacgac ctcgcagccg gctggaaact
      301 cgagccgtgg acgctgcgcg cccggtggca gctcgaggcg cagctcgacg acgccgtccg
      361 ggccgatgtc gacgagcagc accgccgcgc gaaactcgtg cgcccgcgcc ggcgtcgcca
      421 gcagcagcgc cgcgaccacg accgcgaggc gaacccgcag catcagggcc cgcgctccac
      481 gagggcgctc gccaccgccg gcggcacatc tccttcgaaa ctgaccggat agcgctcgcg
      541 tagtccggcg agcgccgcct cgctgctcgc ctcgcggcgc gcggcctccc agtcggcgcg
      601 caggcgcggg cgcacggccg ccagcggcgc gagcgcgggc ggagtcatct catcgacgcg
      661 gacgacatgc cagccgaggt ccgactgcgc gatcgaccat gtccccgggg acatgtccaa
      721 aagcccagcc gcgaagcgct cgccgaacac ccccgcgtac gcggccgccg tgcgcgcccc
      781 gaagcgctgg ccgtgcacac acagatcgcc gagcccgccc gggtcctccc ccgcgagcag
      841 ccgccgctgc aacgcccgcg cgactgcctc ggcgtcggta tgccgctcgc gggccacgaa
      901 cacctgcgtc agcgcgaagc gagcgggcgt cgtgtacctc ccaggatcag cgtcccgcac
      961 ggccagcagc gccgcgtcgt ccggcgccgc gacatcggcg aggtcctcgt gcacgaaccg
     1021 cagcttctgg atcagtcggc ggcgaacgat cgggtcgtcg cgctcgagcc cgagggcgag
     1081 cgcctcgcgg tacagacgct cctcgtcgag ctcggtctgc agcgcgctcg ccagctcggc
     1141 ctccgccggc gcacgaccga gccggcggcc gagctcgttc gcggtcgcgc gcacccgcgc
     1201 cgcgtcgacc accagcgcct cgccgcgcgc gggcgccagc gcggcgtgca gcccgaagaa
     1261 caccaggccg agggcggcga agtgcagcag tggttcggac agcacgcgtc gcggcacggc
     1321 gcgcatcatc gcacctttgc cccgccgcgc tcaagccgcg gcggtcgtca cggctgcttc
     1381 ggggcccgcc ataccacctc ggcgatcggt ctatccgcga gctcggccgg cgtgacctcc
     1441 ccggcgcggg gcttccacgt cgtggggtcg agctcgaagc tgatgttcgc caggtccgtc
     1501 gtcaggcaat acagtcgccg ttccaccctc agc
//
LOCUS       DASWNJ010000370         3322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_78147, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000370 DASWNJ010000000
VERSION     DASWNJ010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78147"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VGB85_06435"
     CDS             complement(<1..296)
                     /locus_tag="VGB85_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0133696.1"
                     /translation="MSDTAEDSRITVLVVDDDKAFCQALAGALRRRNFAVVVAHSYED
                     ALAEAEAWSPDRAVVDLRMPGRSGLELVAALKRVDPHIAVVVLTGYGSIATAIEA"
     gene            complement(368..1729)
                     /locus_tag="VGB85_06440"
     CDS             complement(368..1729)
                     /locus_tag="VGB85_06440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0133697.1"
                     /translation="MSARQSSAQITLRFFVVARWVMLGLVALGWSLQTLSPALFDRLI
                     SWFPPPPEPTGTAAVVILLVATNLWTMRSVLRRGRATTTIAGAHLLVDSVALTALLAL
                     SGGVSNSFTTMYFVPMTLATQLSPRWTWAVAATSLLGFAALFVLAPAPAMHGPPGHEQ
                     HFMGHIRGMWVAFGMSAAFMTYFVHRIALSQARQRAELIRLRLAAAQDRQLAALGTLA
                     AGAAHELGSPLGTVSMLVSDLAFMSDAERSEAIGTIKQELQRCKHILQQMSSPELRVR
                     SLGATAEPWPLARLASDLPHDLRGGSGEPRLQIDQDAAVQAGATTTQPQEVLGQIVRA
                     VVSNAADACKARPGATGVRLRLAVEAGEAAITVSDDGTGMAPQAVAAAFDPFFSTKAE
                     GQGMGLGLYLARAHLRALGGTIDLESQLGHGTTVHVRFPLTEALRGVGARDAAPPDRL
                     SQS"
     gene            1877..2335
                     /locus_tag="VGB85_06445"
     CDS             1877..2335
                     /locus_tag="VGB85_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY0133698.1"
                     /translation="MPKQSAGLLMYRRRGTTLEVLLVHPGGPFWATRDAGAWTLPKGE
                     HNDGEAALDAARREFLEETGVTPEGPFVALTPVRQKAGKVVHAWAFAGDCDPAKLTSN
                     EFTMEWPPRSGKYASFPEVDRAAFFTIAAARDKINPAQLALLEELDRLLA"
     gene            complement(2332..>3322)
                     /locus_tag="VGB85_06450"
     CDS             complement(2332..>3322)
                     /locus_tag="VGB85_06450"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0133699.1"
                     /translation="ALGLGGLGLAMTGALAAERGRVTTLARQLHRMQHDLTSHHSVEQ
                     ELRATHDSLERQALALSDSHRRMEREVEARRRAEAAALEVARIKDSFLHIISHELRTP
                     LNAVIGYTEILLEEDPDVPLRQVRGDHEKIAASADRLLALIDDLLDLAKIEAGKAAVA
                     VESVEVAALAEHVLATVASAAEDSRSTLRLRCEDGLPPLHTDRGKLRRSLLHLLGNAC
                     KFTRDGVVRLTIRQEQEGRPGVVFEVSDTGIGMDAETLGRLFTPFTQGDASTTRRYGG
                     TGLGLAICGHYCNMLGGQIWAESTPGKGSTFTVRLPTERVDPRASGVLVKSAF"
BASE COUNT          467 a         1252 c         1171 g          432 t
ORIGIN      
        1 gcctcgatcg cggtggcgat gctgccgtag ccggtcagca ccacgaccgc gatgtgcggg
       61 tccacgcgct tgagcgcggc gaccagctcg agcccgctgc ggcccggcat gcgcaggtcg
      121 acgaccgcgc ggtcgggcga ccaggcctcc gcctccgcca gcgcgtcctc gtagctgtgc
      181 gcgacgacga cggcgaagtt gcgccgtcgc agggcgccgg cgagggcctg gcaaaaggcc
      241 ttgtcgtcgt cgaccaccag caccgtgatg cggctatcct cggcagtatc gctcatcgcg
      301 ctccccccgg aacggtgccc catgcaccgc ggtccggcaa gcaaatcggc cgcgaactca
      361 cacgaatcta cgactgcgac aatctgtccg ggggcgcggc gtcccgggcc ccgacaccgc
      421 gcagcgcctc ggtcagcggg aagcggacgt gcacggtggt gccgtgaccg agctgggact
      481 cgaggtcgat cgtgccgccg agcgcccgca ggtgcgcgcg cgcgagataa agcccgaggc
      541 ccatgccctg cccctcggcc ttggtcgaga agaacgggtc gaacgcggcc gcgaccgcct
      601 gcggggccat gccggtgccg tcgtcgctga ccgtgatcgc ggcctcgccc gcctcgacgg
      661 ccagccgcag ccgcactccg gtcgcgccgg gccgcgcctt gcatgcgtcg gccgcgttgg
      721 acaccacggc ccgcacgatc tgcccgagca cctcctgcgg ctgggtcgtc gtcgcgccgg
      781 cctgcaccgc cgcgtcctga tcgatctgca ggcgcggctc gccggatccg ccgcgcaagt
      841 catgcggcag gtcgctggcg aggcgcgcga gcggccacgg ctcggccgtc gcgccgaggc
      901 tgcgcacccg cagctccggc gacgacatct gctgcaggat gtgcttgcag cgctgcagct
      961 cctgcttgat cgtgccgatc gcctcgctgc gctccgcgtc gctcatgaac gcgaggtcgc
     1021 tgaccagcat gctgaccgtg cccagcggcg agccgagctc gtgcgccgcc ccggccgcga
     1081 gggtgccgag cgccgcgagc tggcggtcct gcgccgccgc caaccgcagc cggatcagct
     1141 cggcccgctg gcgcgcctgc gacagcgcga tgcggtgcac gaagtaggtc atgaacgcgg
     1201 ccgacatccc gaacgcgacc cacatgccgc ggatgtggcc catgaagtgc tgctcgtgcc
     1261 cgggcggccc gtgcatcgcc ggcgccggtg cgagcacgaa cagcgccgcg aagccgagca
     1321 gcgaggtcgc ggcgacggcc caggtccagc gcggcgacag ctgggtcgcc agcgtcatcg
     1381 gcacgaagta catggtcgtg aaagagttgc tgacgccgcc cgagagcgcg agcagagccg
     1441 tcagcgcgac cgagtcgacg agcaggtgcg ccccggcgat cgtggtcgtc gcgcggccgc
     1501 gtcgcagcac cgagcgcatc gtccacaggt tggtcgccac cagcaggatc acgaccgccg
     1561 cggtgccggt cggctcgggc ggcggcggga accacgagat caggcggtcg aacagcgccg
     1621 gactcagcgt ctgcaaggac cagccgagcg cgacgagccc gagcatcacc caccgcgcga
     1681 ccacgaagaa gcgcagcgtg atctgggccg agctctgcct ggcggacatc gccgcgagca
     1741 tgccacagcg ccgcgaggcc gaccgccccg cggcgatcgg tcgcgcaacc gggccggaac
     1801 ctgcgtccgg cgaaccactg gcccgtggtc gaaggccgct ggtccgccct gccaccctga
     1861 cgttatgctg cgcggcgtgc ccaagcagag cgccggactc ctgatgtacc gccgacgcgg
     1921 gaccaccctc gaggtcctgc tcgtccaccc cggcgggccg ttctgggcca cccgcgacgc
     1981 cggcgcgtgg accctgccca agggcgagca caatgacggc gaggccgcgc tcgacgccgc
     2041 gcgccgcgag ttcctcgagg agaccggcgt caccccggaa ggcccgttcg tcgcgctgac
     2101 cccggtgcgc cagaaggccg gcaaggtcgt gcacgcgtgg gccttcgccg gcgactgcga
     2161 ccccgcgaag ctgaccagca acgagttcac gatggagtgg ccgccgcgct cgggcaagta
     2221 cgcgagcttc cccgaggtcg accgcgccgc cttcttcacg atcgccgccg cccgcgacaa
     2281 gatcaacccg gcccagctcg ccctgctcga ggagctcgac cgcttgctcg cctagaacgc
     2341 cgacttgacg agcacgcccg aggcccgcgg gtccacacgc tcggtcggca ggcgcaccgt
     2401 gaaggtcgag cccttgcccg gcgtgctctc ggcccagatc tggccgccga gcatgttgca
     2461 gtagtggccg cagatcgcca ggccgaggcc ggtgccgccg tagcggcgcg tcgtcgaggc
     2521 gtcgccctgc gtgaacgggg tgaacaggcg gccgagcgtc tccgcgtcca tgccgatgcc
     2581 ggtgtcggag acctcgaaca ccacccctgg ccggccctcc tgctcctggc ggatcgtcag
     2641 gcgcaccacg ccgtcgcgcg tgaacttgca ggcgttgccg agcagatgca gcaagctccg
     2701 gcgcagcttg cctcggtcgg tatgcagcgg cggcaggccg tcctcgcagc gcagccgcaa
     2761 ggtgctccgg ctgtcctcgg ccgcgctggc gaccgtcgcc agcacgtgct cggcgagcgc
     2821 cgcgacctcg accgactcga ccgcgaccgc ggccttgccc gcctcgatct tcgccaggtc
     2881 gagcaggtcg tcgatcaggg cgagcaggcg atcggccgac gccgcgatct tctcgtggtc
     2941 gccccggacc tgccgcagcg gcacgtccgg gtcctcctcg agcaggatct cggtgtagcc
     3001 gatcaccgcg ttgagcggcg tgcgcagctc gtggctgatg atgtgtaaaa acgagtcctt
     3061 gatccgcgcg acctcgagcg ccgccgcctc ggcgcgccgc cgggcctcga cctcgcgctc
     3121 catccggcgg tgactgtcgc tgagcgcgag cgcctgccgc tcgagcgagt catgcgtcgc
     3181 gcgcagctcc tgctcgacgc tgtggtggct ggtcaggtcg tgctgcatgc ggtgcagctg
     3241 gcgcgcgagc gtcgtcacgc gtccgcgttc ggccgcgagc gcgccggtca tcgcgagccc
     3301 gagcccgccg aggcccaggg cc
//
LOCUS       DASWNJ010000371         2295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_466368, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000371 DASWNJ010000000
VERSION     DASWNJ010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466368"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VGB85_06455"
     CDS             complement(<1..349)
                     /locus_tag="VGB85_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0133700.1"
                     /translation="MQEQSLALVTGGNRGIGREIARQLGERGYHVMLAARNAPEGIAA
                     ARELGLEFTPLDVTSQTSIDRLADSLGRRKLGLDVLVHNAGVMYRHFDLEVASNTIAV
                     NFFGPERLTQALLP"
     gene            complement(400..885)
                     /locus_tag="VGB85_06460"
     CDS             complement(400..885)
                     /locus_tag="VGB85_06460"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY0133701.1"
                     /translation="MTTLTTLEISSPLGPLRLLACDGGLCGVYMNIQRAPEATLAPDD
                     PVLQQTARQLQEYFAGTRRDFDLPLRPRGTEFQQTVWRALTQIPYGVTRSYADLAAAI
                     GRPTACRAVGGANSKNPLGIVVPCHRVIGHQGALTGYAGGHNNKRWLLTHEAGAPLVL
                     R"
     gene            complement(882..>2295)
                     /locus_tag="VGB85_06465"
     CDS             complement(882..>2295)
                     /locus_tag="VGB85_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HEY0133702.1"
                     /translation="DACYAALTARDARFDGLFFVGVATTGIYCRPVCVARTPRRDRCS
                     FHRSAAEAERAGFRACFRCRPELAPGHASVDALPRLVASAAALIDDGFLNERGVDALA
                     ATLGVTARHLRRATEEQLGVSPVELAQTRRLALAKQLLQDTDLGLAELAHASGFRSVR
                     RFNALFLARFGRAPSELRRGLAASGHGLSLRLDHREPFDWPRLLGFLAARAIPGVEQV
                     GEHGYRRVVHLGGHTGVVHVRATRGSALRADIDLALAPVLMPLVVRLRRLFDLDADPI
                     RVAEVLARDPALAPAIARRPGLRVPGSVDGFETAVRAVLGQQVSVRAATTLAGRLVAR
                     LGVPISGTHDGLTHRFPTAAELAAVPVAQLAAIGLPATRARTLHTIADAYTRDLPTDP
                     ADFVTTLTALPGIGDWTAQYLVLRALHHPDAFPAGDLVLRKALGDSSEKAARARAEAW
                     RPWRAYAVMHLWTAHAEGPR"
BASE COUNT          323 a          721 c          973 g          278 t
ORIGIN      
        1 gcggcagcag ggcctgcgtc agtcgctcgg ggccgaagaa attgacggcg atcgtgttgc
       61 tggcgacctc gaggtcgaag tggcgataca tgacgccggc gttgtggacc aggacgtcga
      121 ggccgagctt gcggcgcccg aggctgtcgg cgaggcggtc gatgctcgtc tgcgaggtga
      181 cgtcgagcgg ggtgaactcg aggccgagct cgcgcgccgc ggcgatgccc tcgggggcgt
      241 tgcgcgccgc gagcatcaca tgataaccgc gctcgccgag ctggcgggcg atctcccggc
      301 cgatgccgcg gttgcctccg gtgacgagcg cgagggactg ctcctgcatg ccgcgacgat
      361 acgggggcgc gcgcgagaag accagacgcg atcagccgat catcgcagca cgagcggggc
      421 gccggcctcg tgggtgagca gccagcgctt gttgttgtgg ccaccggcgt agccggtcag
      481 cgcgccctga tgcccgatca cgcggtggca cgggacgacg atgccgagcg ggttcttgct
      541 gttggccccg cctaccgccc tgcacgcggt cgggcggccg atcgcggcgg ccagatcggc
      601 gtagctgcgc gtgacgccgt aggggatctg cgtcagcgcg cgccacaccg tctgctggaa
      661 ctcggtgccg cgcgggcgca gcgggaggtc gaagtcgcgg cgcgtgccgg cgaagtactc
      721 ttgaagttgt cgggcggtct gctgcaggac cgggtcgtcg ggggcgagcg tcgcctcggg
      781 cgcgcgctgg atgttcatgt agacgccgca caggccgccg tcgcaggcga gcaggcgcag
      841 gggtccgagc gggctcgaga tctcgagggt ggtcagcgtg gtcatcgggg tccttcggcg
      901 tgggcggtcc acaggtgcat gacggcgtag gcccgccacg ggcgccaggc ctcggcccgg
      961 gcccgcgcgg ccttctcaga gctgtcgccg agcgccttgc gcagcacgag gtcgccggcc
     1021 gggaaggcgt cggggtgatg cagggcgcgc agcaccaggt actgggcggt ccagtcgccg
     1081 atgccgggca aggcagtcag tgtggtgacg aagtcggcgg gatctgtggg caggtcgcgc
     1141 gtgtaggcgt cggcgatcgt gtgcagggtc cgggcgcggg tcgcgggcag gccgatcgcg
     1201 gcgagctgcg cgacggggac ggcggcgagc tcggcggcgg tcgggaagcg atgcgtgaga
     1261 ccgtcgtgcg tgccggagat cggtacaccg aggcgcgcga cgaggcgacc ggcgagcgtc
     1321 gtcgcggcgc gcacggagac ctgctggccg agcacggcgc gcacggcggt ctcgaagccg
     1381 tcgacggagc cgggcacgcg caggccggga cggcgtgcga tcgcaggggc gagcgcgggg
     1441 tcgcgggcga ggacctcggc gacgcggatc gggtcggcgt cgaggtcgaa caggcggcgc
     1501 aggcggacca ccaacggcat caataccggc gcgagcgcga ggtcgatgtc ggcccgcagc
     1561 gcgctgcccc gggtcgctcg cacgtgcacg acgccggtgt ggccgccgag gtggacgacg
     1621 cggcgatagc cgtgctcgcc gacctgctcg acgccgggga tcgcacgcgc ggcgaggaag
     1681 ccgagcaggc gcggccagtc gaagggctcg cggtgatcga ggcgcagcga taaaccatgg
     1741 ccgctcgcgg cgaggccccg gcgcagctcg gagggcgcgc gaccgaagcg cgcgaggaac
     1801 agcgcgttga agcggcgcac gctgcgaaag ccgctcgcgt gcgcgagctc ggcgaggccg
     1861 aggtcggtgt cctgcaacaa ttgtttggcg agcgcgaggc ggcgggtctg ggcgagctcg
     1921 acgggcgaca cgccgagctg ctcctcggtg gcgcggcgca ggtggcgagc ggtgacgccg
     1981 agggtggccg cgagcgcgtc gacgccgcgc tcgttgagga agccgtcgtc gatcaaggcg
     2041 gccgcgctgg cgacgaggcg cgggagggcg tcgacggagg cgtgcccggg cgcgagctcg
     2101 gggcggcagc gaaagcaggc gcgaaagccg gcgcgctcgg cctcggccgc ggagcggtga
     2161 aagctgcagc gatctcgccg cggggtgcgg gcgacgcaga ccggccggca gtagatgccg
     2221 gtggtcgcga cgccgacgaa gaacagaccg tcgaagcggg cgtcacgggc cgtgagcgcg
     2281 gcgtagcagg cgtcg
//
LOCUS       DASWNJ010000372         6881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_394015, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000372 DASWNJ010000000
VERSION     DASWNJ010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394015"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..504)
                     /locus_tag="VGB85_06470"
     CDS             complement(13..504)
                     /locus_tag="VGB85_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133703.1"
                     /translation="MAPVVHDITNVEEDGSIPAAQNPNWSTPGLNNFRQNKQPDSEFG
                     APDAIVAVAPLCQDASYSLVAVVRNVGEASLPAGVVVGFYTGDPPNGTKVGELVTSKA
                     LYPLESEILELPFDAAPDDVKNGIINIYAVVDDTKVPHPTWSECRIDNNIGTSTGKCL
                     VPN"
     gene            complement(999..1562)
                     /locus_tag="VGB85_06475"
     CDS             complement(999..1562)
                     /locus_tag="VGB85_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133704.1"
                     /translation="MKSRATALAPERDLQAELDTNTARARLIASYKDFLSFVQERVGS
                     HALAQGILQDAFVHPDDGLDNPADDESAVAWFVRRLRDAVLDYQRRHEVTTHGLVTFA
                     EELADDAAGAAEVRRVAWRSMARLTRNLDPVYAKALQRIEADSLAVEDYASEVGIKSH
                     NVLVVQVFLAREGLKKQIVLACMTASA"
     gene            complement(1559..2206)
                     /locus_tag="VGB85_06480"
     CDS             complement(1559..2206)
                     /locus_tag="VGB85_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133705.1"
                     /translation="MLARCYRLLLPLLLATGCGKPVDPSGEEASFTKASMGFEPAPSS
                     EPSRPLAADLAIEPVSMLVMFNRLQTAEKLAEVLNRKPGQFSRIDIDKDSAPDPLTVV
                     AMDTADGRAFEIHARPSSGEYVVATMMFDREWAFMGHYNGVMGGAASTVGNPLKAAAA
                     PTTSPTVAPPALAATPGIPPVATPTVAPSGAPSPALIAGSVEAVPASSAIEAGVK"
     gene            2282..2953
                     /locus_tag="VGB85_06485"
     CDS             2282..2953
                     /locus_tag="VGB85_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0133706.1"
                     /translation="MWVAFAPDRLAWDRDHVALVGQSQPGEFVRLPAAMVIRGDDTDE
                     AAVLRAAQRGEQTAQAVLFSRYRDQVARQILRMIGNSASVDDLVQEVFIRTFTALPQF
                     RSDAKLSTWLYTIALNRVRNWWDSQRRRQRREAVATSLPPTEPDEDPEERLETKEHLA
                     RLYAALGVLPDKLREAFVARAIEGMTLLEASQALGRPISTVSYHTRRAEQALCEALGI
                     AYIDK"
     gene            complement(2970..3569)
                     /locus_tag="VGB85_06490"
     CDS             complement(2970..3569)
                     /locus_tag="VGB85_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133707.1"
                     /translation="MRLHSSRHALAMFLSIVPWMGCGGPGGGGVVNDEVAARQAYLGL
                     DRAIDRAIDLGFAGFNAASSANIPEQSQAGDLSGLMVINGKVDQGSSANKGMRLEMTL
                     AQDYADLVLEGDVEVIYNGGPAQLDMSFKGLPNADFSGSLHGTFVMDHDLVGDVTLDL
                     NMSGQTEDAGGIIVRSPGTIRVTGTATSDYGVFNVDVAL"
     gene            complement(3665..4861)
                     /locus_tag="VGB85_06495"
     CDS             complement(3665..4861)
                     /locus_tag="VGB85_06495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0133708.1"
                     /translation="MDNPAFVLASEDDMMIPPAVCGDSVVQRDEVCDDGDADETYCTA
                     MCMLPAPAPNCGDGQPLGEEQCDDGNVAGGDACTEQCLHAFCGDSHLYAGVEQCDDGN
                     FLNDDVCLEDCTLARCGDGHVLAGVEACDDGNRQDDDGCDGDCQFEVLASCGDGVVAG
                     EEECDDMNGEAGDGCSPACRWEIDPSCGNGVIDDGEACDPTASPYDKAVPLCREDCSF
                     AACLIVENTDDVDLDDNEWFSKCAFAAGNRVHVAVVSGGEVLYYARGTKPHPWDAPDK
                     LTSDGPPVQQFRLKSHVPVPLDSPIGGAPGDVLYVFGRASKPPTDGCEESLGDGYGLA
                     IYGENLAPKLLMMSFLGGLSAEPRGFAGWTPAAELSFADDGSLALCSSPGPPPKSATL
                     VVAVTD"
     gene            complement(5023..5856)
                     /locus_tag="VGB85_06500"
     CDS             complement(5023..5856)
                     /locus_tag="VGB85_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133709.1"
                     /translation="MQTFPEALSNAGSIMHAKALFPLLALSLLAGACDAVIVAQADDA
                     PVPASAQAGASADAEVAVVVAEEKTPPAVAVKANVDVQVKPEALDLESITYLVKKSKV
                     KDAAALEKKINNPKEKLNNVDIDGDGKVDKIVIVEVKQDDGIIVFELHAIPSKSKDKD
                     AAVVIAYVNFTPDKTTNVLVVKATYAPVVIGYDTIVYDYTVPIVVKNDVVVVSGGGGF
                     YGWLYAVHRPAFYGVVVYEVAPPPVIIVEYGYDDGGCWPPGHCKHGKWKGGKHGKHGK
                     W"
     gene            complement(6030..6371)
                     /locus_tag="VGB85_06505"
     CDS             complement(6030..6371)
                     /locus_tag="VGB85_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133710.1"
                     /translation="MDSIVVASVLVLLASAVDPVPGVESVASVVVVVAPAEPLAMLVA
                     GSPLVSSVVGELVVMLVVVGSLEVGIKVDAPLDPVLPVSPSTGSGDLQWMVKAPARTV
                     RRVIRDAIQIF"
     gene            6361..>6881
                     /locus_tag="VGB85_06510"
     CDS             6361..>6881
                     /locus_tag="VGB85_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133711.1"
                     /translation="MESTTGPVGPCKPIACDGKTYACGDCEDNDADGKVDLTDPECIS
                     PCDDREDSFATGLPGDNMDPCKQDCFFDGNSGNDACNWNLACDPKNPGGDKCPYNPNK
                     VCEPMQPAECAETCGVPNGCDCFGCCTIVVDGVSHDIFIGDKDCSVANIDTCQQCTKN
                     DDCDDECKPEECEV"
BASE COUNT         1183 a         2265 c         2229 g         1204 t
ORIGIN      
        1 gccgacgcgg cctcagttcg gcaccaggca cttgccggtg ctcgtgccga tgttgttgtc
       61 gatgcggcac tcggaccacg tcgggtgcgg gaccttggtg tcgtcgacga cggcgtagat
      121 attaatgatg ccatttttga catcgtcggg cgcggcgtcg aagggcagct cgaggatctc
      181 cgactcgagc gggtacaggg ccttgctggt caccagctcg ccgaccttgg tgccgttggg
      241 cgggtcgccg gtataaaaac cgacgacgac gccggccggc agcgatgcct cgccgacgtt
      301 acggaccacc gcgaccagcg agtagctggc gtcctggcac aacggcgcga ccgcgacgat
      361 cgcatccggc gcgccgaact cgctgtcggg ctgcttgttc tggcggaagt tgttgaggcc
      421 cggggtgctc cagttggggt tctgcgcggc cgggatgctg ccgtcctcct cgacgttggt
      481 gatatcgtgt acaacgggcg ccatgccccc ctgcgggcac gtttacgaag gtctgtctcg
      541 gaaaccgccg gtttcgcgac aaaatcggta cgattttcgg gacgtccggg gggatcgcgc
      601 tgtcgcgcat gacgtgcttg gggcaggtct agcaggggcc agcggaccag cccgggagcc
      661 gtaaacgatg cggcgaacgt gccaaacaca agcgcgccct gtccgtttgg acgtgaccca
      721 agggacaggc ccggacgttg aataaccacc ggtctcggcg aggtcggcgg gtgcgatcgg
      781 atagccgtgc agatcaccgc cctgggaatg ccgcagttga tcggcctgtg ggggggtcgg
      841 ctccgtgccg ggcgggctac tcgccgaccg cgtggtcggc gctatttcag ttgtgcgttc
      901 gatggcgact gcgccaacgg ccagcgctgt aaagacgaca acttctgctc ctgggattga
      961 ggcccgatgc ggtcgcgggc agggccgcac ggtgggtgct acgcgctggc ggtcatgcac
     1021 gcgagcacga tctgcttctt tagcccctcg cgtgcgagga acacctggac caccaagaca
     1081 ttgtggctct tgattccgac ctcggaagca taatcttcta cggccaaact atcggcctcg
     1141 attcgctgta gcgcctttgc gtataccggg tcgaggtttc tggtgagcct ggccatgctc
     1201 cgccaggcga cgcgacgaac ctcggcggcc cccgcagcat cgtcggcgag ctcctcagcg
     1261 aaggtcacga ggccatgagt cgtgacctcg tgccgtcttt gatagtccaa caccgcatcc
     1321 cgcaagcgtc gcacgaacca cgcgacagcg gactcgtcgt cggctgggtt gtcgagcccg
     1381 tcgtccgggt ggacgaaggc atcttgcaag ataccctggg cgagtgcgtg gctaccgact
     1441 cgctcctgca cgaagctcaa gaagtccttg tagctggcga tcagccgcgc cctggcagta
     1501 ttcgtgtcga gctccgcttg caggtcccgc tccggggcaa gcgcggtcgc gcgtgacttc
     1561 atttgacacc agcctcgatc gcactgctcg ccggcacggc ctcgacgctg ccagcgatca
     1621 gcgcgggact cggcgcaccg gagggggcca cggttggagt cgccacgggg gggatacccg
     1681 gggtggcggc gagcgcgggc ggtgcgaccg ttggcgatgt agtcggggcc gctgccgcct
     1741 tgagcgggtt accgacggtc gaggcggcgc cacccatgac gccgttgtag tgccccatga
     1801 aggcccattc tcggtcgaac atcatggtcg cgaccacata ttcgccactg gacggacgcg
     1861 cgtggatctc gaaagcgcgg ccatccgccg tgtccattgc cacgaccgtc aaggggtcgg
     1921 gcgcgctgtc cttgtcgatg tcgatgcgac taaactggcc cggcttccgg ttgagcacct
     1981 cggccagctt ctccgccgtc tgcagccggt tgaacatcac cagcatggac acgggctcga
     2041 tggccaggtc ggccgcgagg ggacgactcg gttcgctcga cggggccggc tcgaagccca
     2101 tcgatgcctt cgtgaacgat gcttcctcgc cggagggatc cacgggcttg ccgcacccgg
     2161 tggcgaggag gagcgggagc aataaccggt agcagcgagc gagcatggag tgttcctgcg
     2221 tttgggggca tagcgtccga caggctgcgt ttctccagtt ccaacgccgg atagtcttca
     2281 gatgtgggtg gcatttgccc cggacagact ggcctgggac cgagatcacg ttgcgctggt
     2341 cggccagtcg cagccgggcg agttcgttag actgcctgcg gcgatggtga tccgcggcga
     2401 cgacactgat gaggcggcgg tcctccgggc ggcccagcga ggcgagcaga ccgcccaagc
     2461 ggtactcttc tcccgctatc gcgaccaggt cgcgcgccag atcctgcgca tgatcggcaa
     2521 cagcgcctca gtggatgatc tggtgcaaga ggttttcata cgcaccttca ccgccctgcc
     2581 tcagttccgc agcgatgcca agctcagcac atggctctac acgatcgccc tgaaccgggt
     2641 gcgaaattgg tgggattcgc agcgacgcag gcaacggcgc gaggcggttg ccacaagtct
     2701 gccgcccacc gagcctgatg aggatccaga ggaacgtctc gagaccaagg agcatctcgc
     2761 ccgcctgtat gcagcccttg gtgttctgcc ggacaaattg cgggaggcgt tcgttgcccg
     2821 tgcgatcgag ggcatgaccc tactcgaggc ttcgcaagcg ctcgggagac cgatcagcac
     2881 cgtctcgtat cacacgcgtc gagccgagca ggcgctttgc gaagcgctgg gcatcgccta
     2941 catcgacaag tgacatgggg tcggccgcgc taaagcgcca cgtcgacgtt gaacacgccg
     3001 tagtccgagg tcgcggtgcc agtgacgcgg atcgtcccgg ggctgcgcac gatgatgccg
     3061 ccggcgtctt ccgtctggcc gctcatgttg aggtcgaggg tcacgtcgcc gacgagatcg
     3121 tggtccatga caaaggttcc gtgcagactg ccgctgaagt cggcgttcgg caggcccttg
     3181 aagctcatgt cgagctgggc cggtccgccg ttgtagatca cctccacgtc gccctccagg
     3241 accagatcgg cgtagtcctg agcgagcgtc atctcgaggc gcatcccctt gttcgccgac
     3301 gatccctgat cgaccttgcc gttgatgacc atcaagccgg aaagatcgcc ggcctgcgac
     3361 tgctcgggga tatttgcgct gctggcggcg ttgaaacccg cgaagccgag atcgatcgcg
     3421 cggtcgatcg cccggtccag gccgaggtat gcctggcgcg cagcgacctc gtcgttcacg
     3481 accccaccgc ccccgggtcc gccgcagccc atccagggga caatcgagag gaacatggcc
     3541 agcgcatgac gggagctgtg gagtcgcatg ggctcgtcag accgctcccg cggccaactt
     3601 tccaactcat gcgcgagagc gcccagagtt cgtcgcaaac ggtccccggg ccgtacgcgg
     3661 gcggtcagtc ggtcaccgcc acgaccagcg tcgccgactt cggtggggga ccgggcgacg
     3721 agcacagcgc gagcgaccca tcgtcggcga acgataattc tgccgcgggt gtccagccag
     3781 cgaacccccg cggctctgcg ctcaggccgc ccaggaagct catcatcagc aacttcggcg
     3841 ccagattctc cccgtagatg gcgagcccgt atccgtcacc caggctctcc tcgcagccat
     3901 cggtcggcgg tttagaagca cgcccgaaga catataatac gtcgccaggg gcacctccga
     3961 tcgggctgtc gagaggaacg ggcacgtggc tcttgagtcg gaactgctgg accggcggac
     4021 catcactggt cagcttgtcc ggggcatccc agggatgcgg cttggtaccg cgtgcgtaat
     4081 agaggacctc gccgccggac acgaccgcga cgtgaacccg gtttcccgcc gcgaacgcgc
     4141 acttgctgaa ccactcgttg tcatcgagat ccacgtcgtc ggtgttctcg acgatcaggc
     4201 atgcggcgaa cgagcagtcc tcccgacaca gcggcaccgc cttgtcgtac ggcgaggccg
     4261 tgggatcgca cgcctctccg tcatcgatca cgccgttgcc acaactcggg tcgatctccc
     4321 agcgacaggc cgggctgcag ccgtccccgg cttcgccgtt catatcatcg cactcttctt
     4381 caccggccac cacgccgtct ccgcaactgg ccagaacctc gaactggcag tcgccgtcac
     4441 acccatcgtc atcctgccgg ttgccgtcgt cgcaagcctc gacgcctgcg aggacatggc
     4501 cgtccccgca gcgcgcgagg gtgcagtctt ccaggcaaac atcgtcgttt aggaaattgc
     4561 cgtcatcgca ttgctcgacg cctgcgtaga gatgactgtc gccgcaaaaa gcatgcaagc
     4621 actgctccgt gcaggcgtca ccacccgcca cattgccgtc gtcgcattgc tcttcaccca
     4681 ggggttgccc gtccccgcag ttcggcgccg gtgcgggcag catgcacatc gctgtacagt
     4741 aggtctcgtc cgcgtcgccg tcgtcgcaca cctcgtcgcg ctggaccacg ctgtcaccgc
     4801 acaccgcggg ggggatcatc atgtcatcct ccgaggccag gacgaacgcg gggttgtcca
     4861 cggcgcacga caacacgcag ctcagagccc cgagaataag accgcccgct cgccctccct
     4921 cccgcacatt gcgacgatac catgcccgct ggtgccgtgg tgacccgggc gaaggtctgg
     4981 gcgcgtggcg gacgggtctg ggcgctcgcg gcgctggggt cgttaccact tgccgtgctt
     5041 gccgtgtttg ccgcccttcc acttgccgtg cttgcaatga ccgggtggcc agcacccgcc
     5101 gtcatcgtag ccgtactcga cgatgatcac cggcggcggt gcgacctcgt agaccacgac
     5161 cccgtagaac gccgggcgat gcaccgcgta gagccagcca taaaagccgc caccgccgct
     5221 gacgacgacg acgtcgttct tgacgacgat cggcaccgtg tagtcgtaca cgatggtgtc
     5281 gtaaccgatg accaccgggg cgtaggtcgc cttgaccacc aggacgttgg tcgtcttgtc
     5341 gggcgtgaag ttgacgtagg cgatgacgac cgcggcgtcc ttgtctttgg acttcgaggg
     5401 aatcgcgtgc aactcgaaga cgatgattcc gtcgtcctgt ttgacctcga caatgacgat
     5461 cttgtcgacc ttgccgtcac cgtcgatatc gacgttgttg agcttctcct tggggttgtt
     5521 gatcttcttc tcgagcgcgg cggcgtcctt gaccttgctc ttcttgacca ggtacgtgat
     5581 gctctcgaga tcgagcgctt ccggcttgac ctggacgtcg acattggcct tgacggcgac
     5641 ggccggcggc gtcttttcct cggcgaccac gacagcgact tcagcgtccg cagaggctcc
     5701 cgcctgggcg ctcgccggga ccggggcgtc gtcggcctgt gcaacgatga cggcgtcaca
     5761 ggcgccagcg agcagcgaca gggcgaggag ggggaacagg gctttggcgt gcatgatcga
     5821 acccgcattg gacagcgcct cggggaaggt ttgcaaggcg cggggcaggt attagagggg
     5881 gtatgggccc ccgggtccag gtcatgctcg accgacaccg gctcctctcg gcggtgttcg
     5941 cgcatgttcg cgtgcccaaa ggggcgtggc ggtctccggc gctccgtgcg tctcaggggc
     6001 cgatcgcttg gatcttcggt cgttcaccct caaaaaatct gaatggcatc tcgtattacc
     6061 ctcctcaccg tcctcgctgg ggctttgacc atccactgca agtcgccaga cccggtcgag
     6121 ggtgaaactg gcagcaccgg atccagcggc gcgtcgacct tgatgccgac ctcgagcgat
     6181 ccaacgacga ccagcatcac cacgagctcg ccgacgacgg aggatacgag cggcgagcct
     6241 gccaccagca tggccagcgg ctctgcgggc gcgacgacga ccacgaccga cgcgacagac
     6301 tcgacccccg ggaccgggtc gaccgccgac gccagcagca ccagcacgga cgcgaccacg
     6361 atggagtcca cgaccgggcc tgtcggcccc tgcaagccga tcgcctgcga cgggaagacc
     6421 tacgcctgcg gggactgcga ggacaacgat gcggacggca aggtcgacct gacggacccc
     6481 gaatgtatct caccgtgcga cgaccgtgag gacagcttcg ccaccggcct gccgggtgac
     6541 aacatggacc cctgcaagca ggactgcttc ttcgacggca actccggcaa cgacgcctgc
     6601 aactggaacc tcgcctgcga cccgaagaac cccggcggcg acaagtgccc ttacaacccg
     6661 aacaaggtgt gcgagccgat gcagccggcc gagtgcgccg agacctgtgg cgtgcccaac
     6721 ggctgcgact gcttcggctg ctgcaccatc gtcgtcgacg gcgtcagtca cgacatcttt
     6781 atcggcgaca aggactgctc cgtggccaac atcgatacct gccagcaatg caccaaaaac
     6841 gacgactgcg acgacgagtg caagcccgaa gagtgcgagg t
//
LOCUS       DASWNJ010000373         3187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_78213, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000373 DASWNJ010000000
VERSION     DASWNJ010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78213"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..426)
                     /locus_tag="VGB85_06515"
     CDS             complement(7..426)
                     /locus_tag="VGB85_06515"
                     /inference="COORDINATES: protein motif:HMM:NF021593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HEY0133712.1"
                     /translation="MRRGSAVLRRGLKFHFNEIRFTPDATRSRQEDSMRVGIVGGLDR
                     SGPTFQRAARAHGHEVEHHTGDMHGHASVELERLVERADLVVIVTEINSHVAVQLTRR
                     LLRARDRPPVLVRRLGVARLLALLDEHAHTWPTSASA"
     gene            complement(714..2231)
                     /locus_tag="VGB85_06520"
     CDS             complement(714..2231)
                     /locus_tag="VGB85_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133713.1"
                     /translation="MPALRCSSVLVLLTVAALASEAQAGPRTGAKHAASEAREDEPAA
                     TIKPATEPAKAPTRPRESTVTRARARSKASAPVPKDSKVEVPGKPAARPLHPRRDPLL
                     ARHPAATCALDPLEAVGLRQHGRARESLREFYWSDMARFFETAADNADPVPQQVTDPA
                     FVATEQVLRDLPSAVRTPLATIAGARVVDRLGEREFARVSKLLASLAPAQVAGLLRAD
                     APTLRAYVWTWLATTKTGGCHLGHLDLDFIEAAVADRSVAVEHGDDLVYRSLGDYALA
                     ARARLATLDGPRFDAFLVRLTTAAEVDPLVRATAHGVLLRRGHWETQATGLRDASPSV
                     RAATAAAAIEMNRDRVEPTMLEHAAGDPADLVTELIVGALLGVYDPHNQGRPVLRGPL
                     TSTRDERLAATVARWRGRGDPLEPLPAPHRPLRFDAPVRAESVAAAPVPAASPAPTAA
                     EQPVPSTSLASTTPTRPVPSASPVASTEPEPRRPREPSLPGVLDEPDDELPPGAP"
     gene            2362..>3187
                     /locus_tag="VGB85_06525"
     CDS             2362..>3187
                     /locus_tag="VGB85_06525"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0133714.1"
                     /translation="MNATADRYGLVGTIVADKYTIQRVVGEGGFGVVFRAMHRIWDEP
                     VALKCFTALSNAPVEMRDELLDQFVQEGKLLTALSSRTTGIVQARDIGTLTTPGGAWL
                     PYMVLEWLDGKPLEDYLGAKDGEPTGTPRGIFDAFQVMDGPARALALAHARGVAHRDI
                     KPANFFVLGPDLAPGAVIKVLDFGIAKVMQQQSAAALKATGAQISSFTPSYGAPEQFD
                     RSFGATGPWTDVYQMALVMVELMRGGVPALYGEAFMQLAFASQNPDRRPTPRTQGLE"
BASE COUNT          433 a         1033 c         1291 g          430 t
ORIGIN      
        1 cgcggctcag gccgaggcgg aggtcggcca ggtgtgcgcg tgctcgtcga gcagggcgag
       61 caggcgggcg acgccgaggc ggcggaccag caccggcggc cggtcgcggg cgcgcagcag
      121 ccggcgcgtc agctggacgg cgacgtggct gttgatctcg gtgacgatga cgacgaggtc
      181 ggcgcgctcg accagacgct cgagctccac cgaggcgtgc ccgtgcatgt ccccggtgtg
      241 atgctcgacc tcgtggccgt gcgcgcgggc ggcccgctga aaggtgggtc cgctgcgatc
      301 gagtccgccg acgataccga cgcgcatgct gtcctcctgg cgcgaacggg tcgcgtccgg
      361 agtgaagcgt atttcattaa aatggaattt caagccgcga cggagcaccg cgctaccgcg
      421 cctcatgcgc acaaatcggt cggtaggccg tggaagcgcg cggaacgagg gtccgctggg
      481 ggctgtttgc cccgtccatt acaatggaac attttcctta tgatgtcgaa ggccaagcaa
      541 cggatccggc gagaccgcgg cggcgcagcg ccgggccgga gggtcgggcc atgctgacct
      601 tcctgctgat cagcgtgtgg accgcgcggc ggcggcgacg tgcgcgtgac cgcctgatgc
      661 gcgcgcgcga cgaggcgccc gcggcgaacg cctgagcgcg ccgctcggtg ggctcaaggg
      721 gcgccgggcg ggagctcgtc gtcgggctcg tcgagcacgc cgggcagcga gggctcgcgg
      781 gggcggcgag gctcgggctc ggtgctggcg accgggctgg ctgaagggac aggtcgagtc
      841 ggcgtcgtcg acgcgaggct ggtcgaaggg acaggttgct ctgccgcggt cggggcgggg
      901 ctggccgcgg ggacaggtgc ggcggcgacc gactcggcgc ggaccggggc gtcgaagcgc
      961 agcgggcggt gcggggcggg caggggctcg agcgggtcgc cgcggccgcg ccagcgggcg
     1021 acggtggcgg cgaggcgctc gtcccgggtg ctcgtcagcg ggccgcgcag gaccggacgg
     1081 ccctggttgt gcgggtcgta gacgccgagc agggcgccga cgatcagctc ggtgacgagg
     1141 tcggccgggt cgcccgcggc gtgctcgagc atcgtcggct cgacgcggtc gcggttcatc
     1201 tcgatcgcgg ccgcggcggt ggcggcccgc acggacgggc tggcgtcgcg caggccggtc
     1261 gcctgggtct cccagtggcc gcggcgcagc agcacgccgt gcgcggtggc gcggacgagc
     1321 gggtcgacct cggcggcggt cgtcaggcgc acgaggaagg cgtcgaagcg cgggccgtcg
     1381 agggtcgcca ggcgggcccg ggcggccagc gcatagtcgc cgagcgagcg gtagacgagg
     1441 tcgtcgccgt gctcgacggc gacgctgcgg tcggcgacgg cggcctcgat gaagtcgagg
     1501 tcgaggtgac cgaggtggca gccgccggtc ttcgtggtcg ccagccaggt ccacacgtag
     1561 gcgcgcaggg tcggcgcgtc ggctcgcagc agaccggcga cctgggccgg cgcgaggctg
     1621 gcgagcagct tgctgaccct ggcgaattcg cgttcgccga ggcgatcgac gacgcgggcc
     1681 ccggcgatcg tcgccagcgg ggtacgcacc gcggaaggga gatcgcgcag gacctgctcg
     1741 gtcgccacga aggccgggtc ggtgacctgc tgcgggacgg ggtcggcgtt gtcggcggcg
     1801 gtctcgaaga agcgcgccat gtccgaccag tagaactcgc gcagggactc gcgggcccgg
     1861 ccgtgctggc gtaaaccaac ggcctcgagc ggatcgaggg cgcaggtcgc cgcgggatgc
     1921 cgggccagca gcgggtcgcg acgcggatgc agcggccgcg cggcgggctt gcccgggacc
     1981 tcgaccttgc tgtccttcgg gacaggtgcg ctcgccttcg agcgggcacg cgcgcgggtg
     2041 acggtgctct cgcggggcct ggtgggcgcc ttcgcaggct ccgtggcggg cttgatggtc
     2101 gcggcgggct cgtcctcgcg tgcctcgctg gctgcgtgct ttgcgccggt cctgggcccg
     2161 gcctgggcct cggacgcgag cgcggcgacg gtgagcagga cgaggacgga tgagcagcgc
     2221 agggcaggca tgagaccggg agcgaggcta ccggcgggcc gtcggggctg caaaaaaccg
     2281 acgaggcacg ggcagatcgg gatctttgtg ggggcttcaa gtcgtgccag cgcgcggcga
     2341 gtgccttaca atcgcgccac cttgaacgcc accgccgatc gctatggact cgtgggcacg
     2401 atcgttgccg acaagtacac gatccagcgg gtcgtcggcg agggcggctt cggcgtggtg
     2461 ttccgcgcga tgcatcgcat ctgggacgag ccggtcgcgc tcaagtgctt cacggcgctg
     2521 tcgaacgcgc cggtcgagat gcgcgacgag ctgctcgacc agttcgtgca agaaggcaag
     2581 ctgctgacgg cgctgtcgag ccgcacgacc ggcatcgtgc aggcccgcga catcggcacg
     2641 ctgacgacgc ccggcggggc gtggctgccg tacatggtgc tcgagtggct cgacggcaag
     2701 ccgctcgagg actacctcgg cgccaaggac ggcgagccga ccgggacgcc gcgcgggatc
     2761 ttcgacgcgt ttcaggtgat ggatggaccc gctcgcgcgc tggccctcgc gcacgcccgc
     2821 ggggtcgcgc accgcgacat caagccggcc aatttcttcg tgctcggccc ggatctcgcg
     2881 cccggcgcgg tgatcaaggt gctcgacttt ggcatcgcca aggtgatgca acaacagtcc
     2941 gcggccgcgc tcaaggcgac cggcgcgcag atctcgagct tcacgcccag ctacggcgcg
     3001 cccgaacagt tcgaccgcag cttcggcgcg accgggccgt ggaccgacgt gtatcagatg
     3061 gccctggtga tggtggagct gatgcgcggc ggcgtgccgg cgctgtacgg cgaggcgttc
     3121 atgcagctgg ccttcgcgtc gcaaaacccc gaccgccggc cgacgccgcg cacgcagggg
     3181 ctcgagg
//
LOCUS       DASWNJ010000374         2018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_394040, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000374 DASWNJ010000000
VERSION     DASWNJ010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394040"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /gene="hutI"
                     /locus_tag="VGB85_06530"
     CDS             <1..1146
                     /gene="hutI"
                     /locus_tag="VGB85_06530"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEY0133715.1"
                     /translation="AGHSVVIDGERITWVGLEKDRPRTPGATEVDCRGKAVIPALVDS
                     HTHLVWGGDRKDELELRLGGADYEQIFAAGGGILSSVRQTRSIDDDELLAQSRVRLER
                     MRRSGATSFEIKSGYGLDLETELRQLRVARRLAQETGARVRTTCLAAHALPAELRDSP
                     AGRVEFVRRVCDEILPAVAAAGLADFCDVFCDRGAFTPDETRQILLAARALGIAPRLH
                     ANEFGHTGGALLAAELHALSADHLLHLDDAERAALRDAGVVATLLPGTSLVLGKPFAD
                     GRALVKAGVPVAVASDCNPGRCALESLSMVLALACYGCKLSPAEALCGATHNAACALG
                     LADEVGRIDAGTSADLLILATPDYRDLVYHAGSPLISAVYQRGVQVA"
     gene            1321..1773
                     /locus_tag="VGB85_06535"
     CDS             1321..1773
                     /locus_tag="VGB85_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY0133716.1"
                     /translation="MNLNSWNEHSFANLQTQDVLGVTAGQVEGRAAVRAPGPGACASC
                     EPLSTFPCVRVRDDHELVHVLTELPGIDADELEISIDGTMLTLQGRRKENSGLFRVDR
                     GLQKFLCRIELPSAVDSTRGEAYLDMGVLSLTLPKLVCERRRTVKQND"
     gene            complement(1783..>2018)
                     /locus_tag="VGB85_06540"
     CDS             complement(1783..>2018)
                     /locus_tag="VGB85_06540"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY0133717.1"
                     /translation="IIDNTMTTPIIQRHAVSIPPALMMVAQVAMGVLAGGLGVIVAVP
                     LTALVIVLVRRIHVDRLEDELGPNLADADRAGY"
BASE COUNT          301 a          720 c          679 g          318 t
ORIGIN      
        1 gcgggtcaca gcgtcgtgat cgacggcgag cgcatcacct gggtcggcct tgaaaaagac
       61 cgcccgcgca cgcccggcgc gaccgaggtc gactgtcgcg gcaaggctgt gatcccggca
      121 ttggtcgaca gccacacgca cctggtgtgg ggcggcgacc gcaaggacga gctcgagctg
      181 cgcctcggcg gcgccgacta cgagcagatc ttcgccgcgg gcggcgggat cctctcgagc
      241 gtgcgacaga cccgcagcat cgacgacgac gagctgctcg cgcagagccg cgtccgcctc
      301 gagcgcatgc gtcgcagcgg cgcgacgagc ttcgagatca agagcggcta cgggctcgac
      361 ctcgagaccg agctgcggca gctgagggtg gcccggcgtc tggcgcaaga gacaggtgcc
      421 cgcgtccgca cgacctgcct cgccgcccac gcgctgccgg ccgagctccg cgacagcccc
      481 gccggccgcg tcgagttcgt gcgtcgcgtc tgcgacgaga tccttcccgc ggtcgcggcg
      541 gcaggcctcg ccgacttttg tgacgtcttc tgcgatcgtg gagccttcac gcccgacgag
      601 acgcgccaga tcctgctcgc tgcccgcgca ctcgggatcg caccgcgcct gcacgccaat
      661 gaattcggcc ataccggcgg ggccctgctc gccgcggagc tgcacgcgct gtccgccgac
      721 cacctgctcc acctcgacga cgccgagcgc gccgccctgc gtgacgccgg ggtcgtcgcc
      781 acgttgctgc ccggcacctc gctcgtgctc ggcaagccgt tcgccgacgg ccgcgcgctg
      841 gtcaaggccg gcgtgccggt cgcggtcgcc tccgactgca accccggcag gtgcgccctc
      901 gagagtctct cgatggtgct cgcgctcgcc tgttacggct gcaagctctc gcccgcggag
      961 gccctgtgcg gcgcgacgca caacgccgcg tgcgcgctcg gcctcgcgga cgaggtcggc
     1021 cgcatcgacg ccggtaccag cgccgacctc ctgatcctcg ccacgcccga ctaccgcgac
     1081 ctcgtctatc acgccggctc gcccctgatc tcggccgtgt atcagcgcgg ggtccaggtc
     1141 gcctgagacg ctgccgacgc cgctttgtat ttgatagtta taagtccggt gcgaggaatg
     1201 acgggaatgc aggcacaatt ctcttgacgg tgtaacgaga attttccctg gcctcgtctt
     1261 tctctcgggt gcacactcac atgacggcat atcgaggtgc cgcttgctgg agcattcgcc
     1321 atgaacctca actcctggaa tgagcactcc tttgcgaact tgcagaccca ggatgtgctc
     1381 ggagtgacgg ccggccaggt ggagggtcgg gcggcagtcc gcgcgcccgg tcctggtgcg
     1441 tgtgcgtcgt gtgagccgct gtcgaccttc ccgtgcgtgc gcgtgcgcga cgaccacgag
     1501 ctcgtgcacg tgctgaccga gctgccgggc atcgacgccg acgagctcga gatctcgatc
     1561 gacggcacga tgttgaccct gcagggccgg cgcaaggaaa acagcgggct gttccgggtc
     1621 gaccgcggcc tgcaaaaatt cctgtgccgg atcgagctgc cgagcgccgt cgactcgacg
     1681 cgcggcgagg catacctcga catgggcgtg ctctcgctga cgctgcccaa gctggtgtgc
     1741 gagcgccggc ggaccgtcaa gcagaacgac tgacggccgg cgctagtacc ccgcgcggtc
     1801 ggcgtcggcc aggttcggtc cgagctcgtc ctcgagccgg tcgacgtgga tcctgcggac
     1861 cagcacgatg acgagggccg tcaacggcac cgccacgatc acgccgaggc ctccggcgag
     1921 cacgcccatc gccacctgcg cgaccatcat cagggccggc gggatagaca ccgcgtggcg
     1981 ctgaatgatc ggcgtcgtca tcgtgttgtc gatgatct
//
LOCUS       DASWNJ010000375         6608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_7341, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000375 DASWNJ010000000
VERSION     DASWNJ010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6608
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7341"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2023
                     /locus_tag="VGB85_06545"
     CDS             <1..2023
                     /locus_tag="VGB85_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133718.1"
                     /translation="AELHVRLADDPGAASGLHALGAALELLGKVHDELALGASPFGYP
                     AAYVPLALGPEDIAHNRSNFEAVQVLAADEVMQFGQVAADAWQRARDYEQKIHTLEVT
                     ASQIAAEYDGKLRALCGSLPGETAPALATCGQNGGQIADLRAAVQAAGLRIRHAGQAS
                     ENNLHSIVVEENRFGRVVALAFSLSEEIDAAQGRIFQVKDKYGEHRSTVQQAEAMAEC
                     GRIRSNASAEKAALEAGCDSAMVAQMTAGPVILGVSTPNPMGLIVASKECEAKRESVE
                     KQTENQCLSVLGHVGLQNSLDDLQRNEDEAITAINAEIDQAIRNSDLEVQRAASVALI
                     RNIRAEGLLLQIETEEAELAHSTAMTAMWSAYMEAAALMQEKARAMGLMVEDSPDNAL
                     THPHFLQARLEAAGRVLPARERTIRRVYLALRALEYELNQELPSLRETLAAARSPEDI
                     TGLMTCLASIAEDYRLEHGYGQPYVTEVSLRADIFGMTTDVPDVDGSPATPAEQFAAL
                     LQDPLHGQADGSVALPFALSAYENAQFSTLLCDDRIDKIEVKLVGDFLGDKEAEVMLT
                     RQGLAGVRRCDGADLPQWSAYVPYGFEREQIVIQAGVNDWGTAGPNAGYAAWPVHGEQ
                     WTLTIPPPEQAPANLDLDLAHVSDVVLRLHHRAGTIAPAGQGTFTPSCG"
     gene            2061..2255
                     /locus_tag="VGB85_06550"
     CDS             2061..2255
                     /locus_tag="VGB85_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133719.1"
                     /translation="MQPGLATIVVSLPGRTIRSRVFMVKLRHDVVDVAFTSGDMITLM
                     ARTAVGCIEPPAAKPRRRAS"
     gene            2347..>6608
                     /locus_tag="VGB85_06555"
     CDS             2347..>6608
                     /locus_tag="VGB85_06555"
                     /inference="COORDINATES: protein motif:HMM:NF023677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin TcdB middle/N-terminal domain-containing
                     protein"
                     /protein_id="HEY0133720.1"
                     /translation="MLNSKSCARFVLGLGALFLPAHVLADTSATLATAINIPKGPASI
                     EGFGRAYEVSPASGLPSLEYPIAVPPGRAGHGPALALHYHAGTGAGVLGLGWSLDLPA
                     IERSPRAGNPRPGEPAVWTLRHLGDGEELAETAPGVYRQRIEQGAPIVVRALPGGAMS
                     AVATDGTGYLFGLTDEARLDSDAGPVHLELSAITDVHGNRIDFSYTRIAGSDAPLLAA
                     ITWNDGRARILFDYDARPDVVTSRALGVRVALAHRLANIRTEAAGEPVRTTTLTYARS
                     VEAPSSRLASIATVAADGAGLPAWRITYTGEAATPVARDVPGGPALDPTADGRAWVDV
                     DGDALPDLLDGEPGAWRMRKNTGGQLATTWTTLASPAAALGPNARFADLTGDGVGDLL
                     AEKSPGELWSYVGGGADPFGHAEPTPVDLSFDLSDPRVALADMNADGQVDVLRHDDED
                     GWIWLRRRGGGYEAAEAVPPPPAGMRLGDPGVQLADMDGDRIPDLVRILAADSRILVA
                     PGAGLGLFDEPAELHGVPTMQETDRFELADMNGDGAADLVRIGADQLRVWLAQLDGGF
                     AESMATDWSTLDADEIVLVTDIDGSGTPDVLRIDTDGSQPWRVWTLFPERPGLLARFE
                     NGLGYVREHIYRSAAQLAAEDAAAGSPWTTTPPEPMPVLTETREDDGAGWTSTLRRGL
                     RDGWYDPTRGEFRGFAELTDRTTGDAYSESATIARRYDLGQTDDARGLQLVAAETRSP
                     RGMLVREAHTLAVDSPASGVRVVRRTATDTYHVESGPEAAAVRVRTEWDHDDWANVIE
                     ERALGRVDRESGADIPGDERITTTTYATPRAKDGPRTRVAEQVITDAEGAQITATRTH
                     YDGPPEEGLPLGELDARGVVARTLTWTKGDTWVLSLRQTVDSHGNVTRVRDAEDGTLV
                     RLWDAAGLFPVEERLQLAAGEALVTAAEWDARSGHPVTVTGPSGAPTRAVYDGLGRLV
                     AEIEPGDSAELPTTRHVYHLDGAAVRPSVITELRRQSGEPDVDVVALHLDGLGRPRLR
                     VTQDDTGEAAILAEARIYSDAGALAELIEGQPLPAAALAPGAVVDLAVDWPRSIRRAD
                     ALGRVVFTRDADDRETAVTFGPLVSLHRDHADLHPKAPYQDTPERIVQDGLGRTVARE
                     ALLTDRTIRHAYEHDAAGRLAAHVDPAGHHTTWEHDGAGRLTRVQSPDAGEVRQRFDG
                     VGRVTERTDATGARVTWSYDPLGRLLRERAFSPTGEPAGEVRHTYDGEGPHARGQLAR
                     VDDDAGGVTFTYDARGRIVSSTRTFAAASGPVTLTAGQVYDAQDRIVRDIYPDGSTLD
                     HDYSPRGLERPLPGFVTGVDYDALARWRSITLAPGAELHRDLDPAGRVLGQHVTAGSR
                     SLLRLTHRYDAAGQLAETRDDLVAEGLTLAQTFVYDDLRRLIG"
BASE COUNT          991 a         2569 c         2213 g          835 t
ORIGIN      
        1 cgccgagctg cacgtgcgcc tcgccgacga ccccggcgcc gcgtcgggct tgcacgcgct
       61 cggtgctgcg ctcgagctac tcggcaaggt ccacgacgaa ctcgcactcg gtgcatcacc
      121 cttcggctac cccgcggcat atgtcccgct ggcgctcggc cccgaggaca tcgcccacaa
      181 ccgcagcaac ttcgaggccg tgcaggtgct cgcggccgac gaggtcatgc aattcggaca
      241 ggtcgccgcc gacgcatggc aacgggcccg cgactacgaa caaaagatcc acactctcga
      301 agtcaccgcg tcgcagatcg cggccgagta cgacggcaag ctgcgcgccc tgtgcggcag
      361 tctgcccggc gagacggcgc ccgcgctggc cacctgcggc cagaacggcg gccagatcgc
      421 cgacctacgc gcggcggtcc aggcggcggg tctacgcatc cgccacgccg gccaagccag
      481 cgagaacaac ctgcactcga tcgtggtgga agagaatcgt tttggtcgtg tggtcgccct
      541 ggcgttcagt ctgagcgagg agatcgacgc ggcgcagggc cgcatcttcc aggtcaagga
      601 caagtacggc gagcaccgct cgaccgtgca gcaggccgag gccatggccg agtgcggtcg
      661 catccgcagc aacgcctcgg ccgagaaagc ggccctggag gccggctgcg acagcgcgat
      721 ggtcgcccag atgaccgcgg gtccggtgat ccttggcgtc tccacgccga acccgatggg
      781 cctcatcgtc gccagcaagg agtgcgaggc caagagggag agcgtcgaga agcagactga
      841 aaaccagtgc ctgagtgtgc tcgggcacgt gggcctgcaa aactcgctcg acgatctcca
      901 gcgcaacgag gacgaggcga tcacggcgat caacgccgag atcgatcagg cgatccgcaa
      961 cagcgatctc gaggtccagc gcgcggcgtc ggtcgccctc atcaggaaca tccgggccga
     1021 gggtctgctg ctccagatcg agaccgagga ggccgagctg gcgcacagca ccgccatgac
     1081 ggcgatgtgg agcgcgtaca tggaagccgc cgcgctcatg caggagaagg cccgcgccat
     1141 gggcctgatg gtcgaggaca gccccgacaa cgccctgacg cacccgcact tcctgcaagc
     1201 ccgccttgag gccgccggcc gcgtcctgcc cgcgcgcgag cggacgatcc gccgtgtgta
     1261 cctggcgctg cgtgccctcg agtacgagct gaatcaagag ctgccgtccc tgcgtgagac
     1321 cctcgccgcg gcgcgctccc ccgaggacat caccggcctc atgacctgcc tcgccagcat
     1381 cgcggaggat tatcgtctcg agcacggcta cggccagccc tacgtcaccg aagtcagcct
     1441 gcgcgccgac atcttcggga tgacgaccga cgtcccggat gtcgacggca gccccgcgac
     1501 cccggccgag caattcgccg cgctcctgca agaccccctg cacggccagg cggacggcag
     1561 tgtcgcgctg cccttcgcgc tctcggccta cgagaacgcc caattctcga cgctgctgtg
     1621 cgacgaccgg atcgacaaga tcgaggtcaa gctcgtcggc gacttcctcg gcgacaaaga
     1681 ggctgaggtc atgctgaccc gtcagggcct cgcaggcgtg cgtcgctgcg acggcgccga
     1741 cctcccgcag tggtcggcct acgtgcccta cggcttcgag cgcgagcaga tcgtcattca
     1801 ggccggcgtc aatgattggg gcaccgcggg cccgaacgcg ggctacgccg cctggcccgt
     1861 gcacggtgag cagtggactc tgacgatccc gccgcccgag caggcaccgg caaacctcga
     1921 cctcgacctc gcacacgttt ccgacgtcgt gctgcgcctg catcaccgcg ccggcaccat
     1981 cgcccccgcg ggtcagggca ccttcactcc gagctgcggg tgatgcaatg actacgccac
     2041 gcatcatccc cgcctccgcc ttgcagccgg gcctggcaac gatcgttgtc tcgctccccg
     2101 gtcgaacgat ccgcagccgc gtgttcatgg tgaagctgcg ccacgacgtc gtggatgtcg
     2161 cattcacctc cggcgacatg atcacgctca tggctcgcac ggcggttggc tgcatcgaac
     2221 cgccagccgc caaaccccgt cgccgcgcgt cctgacgcgc acgagccgac cccgtgcccg
     2281 taggcggcgc gggggtcctg atatccacca gagatccgtt agcgatcaga aacccgagag
     2341 gtcgccatgc tgaattcgaa gtcctgcgcc cgcttcgtgc tggggctggg ggcgctgttc
     2401 ctgcccgcac atgtgctcgc cgacaccagc gccacgctcg ccaccgccat caacatcccg
     2461 aagggccccg cttcgatcga gggattcggg cgcgcctacg aggtcagccc cgcgagcggc
     2521 ctcccgtcgc tcgagtaccc gatcgccgtc ccacccggcc gcgccggcca cggccctgcg
     2581 ctggcgctgc actaccacgc aggcaccggc gccggggtgc tcggcctcgg ctggtcgctc
     2641 gacctgccgg ccatcgaacg ctcgccgcgc gccggcaacc cgcggcccgg cgagccagcg
     2701 gtctggacgc ttcggcacct cggcgacggt gaggaactgg ccgagacggc gcccggcgtc
     2761 taccgccagc gcatcgagca gggggcgccc atcgtcgtgc gcgcgctgcc gggtggggcc
     2821 atgtcggccg tcgccacgga cggcaccggc tacctgttcg gcctcacgga cgaggcccgg
     2881 ctcgattcgg acgcggggcc ggtccacctg gagctgtcgg cgatcaccga cgtccacggg
     2941 aaccgcatcg acttcagcta cacccgcatc gccgggagcg acgcgccgct gctcgccgcg
     3001 atcacctgga acgacgggcg cgcccgcatc ctcttcgact acgatgcccg ccccgacgtc
     3061 gtcacaagcc gcgccctggg cgtccgcgtc gcgctggcgc accgcctcgc caatatccgc
     3121 accgaagccg cgggcgagcc ggtgcggacg acgaccctga cctacgcacg cagcgtggag
     3181 gcgccgtcca gccggctcgc gtcgatcgcc accgtcgcgg cggacggcgc ggggctgccg
     3241 gcctggcgca tcacctacac cggcgaggcc gccacgcccg tggcgcgcga cgtgccgggc
     3301 ggccctgcgc tcgatccgac cgcggacggc cgcgcgtggg tcgatgtcga cggcgacgcg
     3361 ctccccgatc tgctcgacgg cgagccgggc gcgtggcgca tgcgcaagaa caccggcggc
     3421 cagctcgcta cgacctggac cacgctcgca tcgcccgctg cggcactcgg gccgaacgcc
     3481 cgcttcgccg acctcaccgg cgacggggtg ggcgacctgc tcgcggagaa gtcccccggc
     3541 gagctgtgga gctacgtcgg cggcggtgcg gacccgttcg ggcacgccga gccgacgccg
     3601 gtcgatctga gcttcgactt gtccgaccca cgcgtcgcgc tcgccgacat gaacgcggat
     3661 ggccaggtcg acgtgctccg ccacgacgac gaggacggct ggatctggct gcggcggcgg
     3721 ggcgggggct acgaggcggc cgaggccgtc ccgccgccgc ccgccgggat gcggctcggc
     3781 gacccgggcg tgcagctcgc cgacatggac ggcgaccgca tccccgatct cgtccgcatc
     3841 ctcgcggccg acagccgcat cctcgtcgcg cccggcgcgg ggctcggcct cttcgacgag
     3901 cccgccgagt tgcacggcgt gccgacgatg caggagacgg accgcttcga gctcgccgac
     3961 atgaacggcg acggcgcggc cgacctcgtg cgcatcggcg cggaccagct ccgggtctgg
     4021 ctcgcacaac tggacggcgg cttcgccgag tccatggcca ccgactggtc cacgcttgac
     4081 gccgacgaga tcgtgctcgt caccgatatc gacgggagcg gcacccccga cgtgctccgc
     4141 atcgacacgg acggctcgca gccgtggcgc gtctggacgc tgttccccga gcgcccgggc
     4201 ctgctcgcgc gctttgagaa cggcctcggc tacgtccgcg agcacatcta ccgctccgcc
     4261 gcgcagctcg ccgccgagga cgccgcggcc ggttcgccct ggaccacgac gccgccggag
     4321 ccgatgccgg tgctcaccga gacgcgcgag gacgacggcg ccggctggac aagcacgctt
     4381 cgccggggcc tgcgcgatgg ttggtacgac cccacccgcg gcgagtttcg cggattcgcc
     4441 gagctgaccg accggaccac gggcgacgcc tacagcgagt ccgcgaccat cgctcgccgc
     4501 tacgacctgg ggcagacgga cgatgcgcgc ggcctgcagc tcgtcgccgc cgagacccgc
     4561 agcccgcgcg gcatgctcgt ccgcgaggcc catacactcg cggtggacag ccccgcgtcc
     4621 ggcgtccgag tggtccgccg caccgccacg gacacctacc acgtggagtc cggtcccgag
     4681 gccgccgccg tccgcgtgcg taccgagtgg gaccacgacg actgggcgaa cgtgatcgag
     4741 gagcgggcgc tcggccgcgt cgatcgcgag agtggcgccg acatccccgg cgacgagcgg
     4801 atcacgacga ccacctacgc gacgccccgc gcgaaggacg gcccacgcac ccgcgtcgcc
     4861 gagcaggtca tcaccgacgc ggagggcgcc cagatcaccg cgacccgcac ccactacgac
     4921 ggcccgcccg aggagggcct cccgctcggc gagctggacg cgcgcggcgt cgtcgcccgc
     4981 accctgacat ggaccaaggg cgacacctgg gtcttgtcgc tgcggcagac cgtcgactcc
     5041 catggcaacg tcacccgcgt ccgcgacgcg gaggacggca cgctcgtgcg gctgtgggac
     5101 gccgcgggcc tttttccggt ggaggagcgt ctccagctcg cggccggcga ggcgctcgtc
     5161 accgccgccg agtgggacgc gcgcagtggc cacccggtca cggtcacggg tccgagcggc
     5221 gccccgaccc gcgcggtcta cgacggcctc ggccgcctgg tcgccgagat cgagcccggc
     5281 gactccgccg agctgccgac gacccgccac gtctaccacc tggacggcgc cgccgtgcgg
     5341 ccgtccgtga tcacggagct gcgtcgccag agcggcgagc cggacgtcga tgtcgtcgcg
     5401 ctacacctcg acggcctcgg tcgcccgcgg ctgcgcgtca cgcaggatga caccggcgag
     5461 gcggcgatcc tcgccgaagc acggatctac agcgacgcag gcgcgctcgc cgagctgatc
     5521 gagggccagc cgctccccgc cgccgcgctg gcgcccggcg ccgtggtcga cctcgccgtg
     5581 gactggcccc gcagcatccg ccgcgccgac gcgctcggcc gcgtcgtctt cacccgcgac
     5641 gcggacgacc gcgagacggc cgtgaccttc ggcccgctcg tctccctgca ccgcgaccac
     5701 gcggatcttc acccgaaggc gccgtaccag gacacgcccg agcgcatcgt gcaggacggc
     5761 ctcggccgca cggtcgcccg cgaggcgctt ttgacggacc gcaccatccg ccacgcctac
     5821 gagcacgacg ccgccggccg tctggccgcg cacgtcgacc ccgccggaca ccacacaacg
     5881 tgggagcatg acggcgccgg ccgcctcacc cgcgtgcagt cgcccgacgc tggcgaggtc
     5941 cgccagcgct tcgacggggt cggccgcgtc acggagcgaa cagacgccac cggcgcgcgc
     6001 gtaacctggt cctacgaccc gctcggccgg ctcctgcgcg aacgcgcatt tagccccacc
     6061 ggcgagccgg caggcgaggt tcggcacacc tacgacggcg aggggcccca cgcgcgcggc
     6121 cagctcgcac gcgtcgacga tgacgcgggc ggcgtcacgt tcacctacga cgcccgcggc
     6181 cgcatcgtct cgtccacgcg gacatttgcc gccgccagcg gccccgtcac cctcacggcc
     6241 gggcaggtct acgacgccca ggatcgcatc gtccgcgaca tctacccgga cggcagcacc
     6301 ctcgatcacg actactcgcc ccgcggcctc gaacggccgc tcccgggctt cgtgaccggc
     6361 gtcgactacg acgcgctcgc ccgctggcga tccatcacgc ttgcgcccgg cgccgagctg
     6421 caccgcgacc tcgaccctgc gggccgcgtg ctcggccagc acgtgaccgc cggctcgcgc
     6481 tcgctgctcc gcctcaccca ccgctacgat gccgccggcc agctcgccga gacccgcgac
     6541 gacctcgtgg ccgaggggct caccctcgcg caaaccttcg tctacgacga tctgcgccgc
     6601 ctcatcgg
//
LOCUS       DASWNJ010000376         2155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_147836, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000376 DASWNJ010000000
VERSION     DASWNJ010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147836"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VGB85_06560"
     CDS             <1..475
                     /locus_tag="VGB85_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEY0133721.1"
                     /translation="KLYQTDATARRLLRSQYRDRGHRADGPGNVTMKHSIRTSVLVAL
                     GMLVALGPVACDHDCDSCGHNEPAAPYGLEAEVRADGVALAWTDASDDEDDFIVERSV
                     KDAAALHFVDLVELPENTEDYLDTDVDAGVTYVYRIKAVNGSASSISDELEVMLP"
     gene            551..901
                     /locus_tag="VGB85_06565"
     CDS             551..901
                     /locus_tag="VGB85_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133722.1"
                     /translation="MIYDASSSCPDQSLEEAVTMAAHHDEVGAQLLGDVDDDLTGVPY
                     AAEALTPHNRRVGRVEGLDQRRELLGSLSEQIRSELFTGEDPDVRGHIGRDMDSPAQM
                     ERGPGSGGEIGRLA"
     gene            complement(996..1622)
                     /locus_tag="VGB85_06570"
     CDS             complement(996..1622)
                     /locus_tag="VGB85_06570"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEY0133723.1"
                     /translation="MCDRAVVARRLLHMWRRARRLRHRGASAVPVTRRRMRSIKKFSI
                     LVLSASAILACDTHHDAGATSPRSDVEFIDAMIPHHEMALEMTDVVLQKGSDAEVKAM
                     AKAMKDAQTTEIAEMKAIRTRLAGSAATTAQHDEHMMADMEKLEAASGAALDRLFLEE
                     MLPHHAGAIKMAHEALPYLEETELQVMANKIVHDQAKEIGDIAAMLQE"
     gene            1744..>2155
                     /locus_tag="VGB85_06575"
     CDS             1744..>2155
                     /locus_tag="VGB85_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133724.1"
                     /translation="MRRSDVFIVLTALASSGFACHSKAPAQATEVPAGAPHNGGESSC
                     RHELGRCGGHKAGDGACGGEVAASSEPDAASPTPLDDVVVEPGKFAEINFEMAAGSAT
                     DVTFEAVGGSLEWNVHSHDGDKVAIHAEGTGADGK"
BASE COUNT          366 a          695 c          715 g          379 t
ORIGIN      
        1 caagctgtat cagacagatg cgacggcacg gcgtttgctc aggagccagt acagggatcg
       61 aggccatcgc gccgacggtc caggaaatgt cacaatgaaa cactcgattc ggacctctgt
      121 gctcgtcgca cttggcatgc tcgtcgccct cgggcctgtt gcctgcgatc atgactgcga
      181 ctcctgcggg cacaacgaac ccgccgcccc gtatggcctt gaggccgaag tgcgggcgga
      241 tggcgttgcg cttgcgtgga cagacgcctc cgacgatgag gacgacttca tcgtcgagcg
      301 ctcggtgaag gatgcggccg cgttgcactt cgtcgatctg gtcgaattgc ccgaaaatac
      361 cgaggactac ctggacaccg acgtcgacgc gggcgtgacc tacgtgtacc gaatcaaggc
      421 cgtgaatggc tcggcttcgt cgatctcgga cgagctcgag gtcatgctgc cgtgaaccga
      481 cgagggtgct cctcgccggc tcaatgcact acgccggagc tcctcggacc acgagaacgc
      541 tacagcgcgc gtgatttacg atgcgagcag cagttgtccc gaccagtcgc tcgaggaagc
      601 cgtaaccatg gctgcccatc atgatgaggt cggcgcccag ctcctcggcg acgtcgatga
      661 tgacctgacc ggggttccct acgcggcaga ggcgttgacc ccacacaacc ggcgtgtggg
      721 gcgggtggag ggcctggacc agcgccgtga gctcctcggc tccctgagcg agcagatacg
      781 cagtgagctg ttcaccggcg aggatccaga cgtccgcggg cacatcgggc gggacatgga
      841 cagcccggca cagatggagc gtggccccgg ttcgggcggc gagatcggcc gcctggcgta
      901 ggacctgtgg cgccctgggc gatccgtcga gggcggcgag gatgtgcttg tacatgtgag
      961 acggcgactt gcgtaccgga gttgcgaccc tccgtctact cctggagcat cgccgcgatg
     1021 tcgccgatct ccttcgcctg gtcgtggacg atcttgttgg ccatcacctg cagctcggtc
     1081 tcctcgagat acggcagcgc ctcgtgagcc atcttgatgg cgcccgcatg atgcggcagc
     1141 atctcctcga ggaacaggcg atccagcgcg gcacccgacg ctgcctccag cttctccata
     1201 tcggccatca tgtgctcgtc atgctgcgcc gtagtcgcgg ccgaccctgc gagccgcgtc
     1261 cgaatggcct tcatctcggc gatctcggtg gtctgggcgt ccttcatcgc cttggccatc
     1321 gccttgactt ccgcgtcgct gcccttttgc agcaccacgt cggtcatctc gagggccatc
     1381 tcgtgatgcg ggatcatggc gtcgatgaac tcgacatcgg acctcgggct cgtggcgccg
     1441 gcatcatgat gcgtgtcgca cgcgaggatc gccgaggcgc tcagtaccag gatggagaac
     1501 ttcttgatgg agcgcatgcg tctgcgtgtc acgggaacag ccgatgcgcc acggtgacga
     1561 agtctgcgag cgcggcgcca catatgaagc agccgacgtg caaccaccgc gcggtcacac
     1621 atgtctcagc cgacttgagg atattctcaa gcgaccgcgg cggccgtcgc ctgtgtcgca
     1681 gattcaccag cgatcctagc cttgcggagg ctggcacggg atctgctagg tcagggggaa
     1741 ttcatgcgcc gctccgacgt ctttatcgtt cttaccgcac tcgccagtag cggcttcgcg
     1801 tgccattcca aggccccagc ccaggcgacc gaggtccccg cgggcgcgcc gcacaacggc
     1861 ggcgagagct cttgtcggca cgagctcggt cgctgcggtg gtcacaaggc gggcgacggc
     1921 gcatgcggtg gcgaggtcgc ggcgagctcg gagcctgatg ccgcgagccc gaccccgctc
     1981 gatgacgtgg tcgtcgagcc gggcaagttc gccgagatca acttcgagat ggcggccggc
     2041 tccgcgaccg acgtgacgtt cgaggccgtg ggcgggtcgc tcgagtggaa cgtacacagc
     2101 cacgacggcg acaaggtcgc catccacgcc gagggcaccg gcgccgacgg caagg
//
LOCUS       DASWNJ010000377         3604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_642669, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000377 DASWNJ010000000
VERSION     DASWNJ010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642669"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VGB85_06580"
     CDS             complement(<1..415)
                     /locus_tag="VGB85_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133725.1"
                     /translation="MTSSFARLSLVASLLSACTVKLGDDPEGSSSSSSAADTDPTVGH
                     SSAPTGDGPDATTDPPSTSNDSVVIPETTTGVEDHTTGDFTTGAFTTGDTDGGEPSVC
                     AQMCEHSAQCGLSADAGACTIGCEDALADSPPACAA"
     gene            complement(539..949)
                     /locus_tag="VGB85_06585"
     CDS             complement(539..949)
                     /locus_tag="VGB85_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133726.1"
                     /translation="MTRLHSFSKTLVCVMLAGGLTPAPADAKLPPPETDFNEQQQVNP
                     ARPPRVVLVAQDSVTVIEIFDCAYQQNVGACIDSAFAGSGTSLAAIDTNRIGRFLQGT
                     ARVSAMSGPRAIANANYSYDNLLIEGDIKIRAQE"
     gene            complement(1132..>3604)
                     /locus_tag="VGB85_06590"
     CDS             complement(1132..>3604)
                     /locus_tag="VGB85_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0133727.1"
                     /translation="LKPDNILVGKDGRVRVVDFGLARRSDFKLESLAERSHLNAANAS
                     QVSGLFDVSHTMVGSLVGTPAYMPPEQLDSGEIDARSDIFSFCVSLFEALYGQRPFTG
                     DDADELRQAIVAGDMAELPRDAIVPARVHKVVLRGLAADRKDRPPSMAELLAGLRTAI
                     DRPRRIWFAALGALSVVSLAVGLIGAAALQRSASAELCTGAPARLIGVWDPPARAALE
                     HAVLASGLSYAPATWVRLAARLDRYTEAWTAMHGEACLTHHRGEQSDELFERRMTCLD
                     DRLAEVAAVIDVLKTADRTAIERAVQATEAISTLDRCVDSSLLMAQTPPPTPTVAAAA
                     ADLRERVARAKAEESAGRYPAALAQIDAVVTDAQALAYPPVIAMALHRRASVQERMGE
                     YVAAEASGFAALAAAETAGDDGLRARIGTALIQLTGPRMKHFDEALHLSAQIGGLLAR
                     VDQDPEVRIQFLGSTGNVHFARGALPEARATLAEALALAERSFGPDDPRLVPSLSGLG
                     NVAVTEGRPAEALAFYRRGLAIWETSLGPEHPRLVALLTNIVASANADPASRPLAVES
                     AYRAVALAERALGPEHPETLATLSSLGQTLIWQDKPGAAEPVLRRLMAARERALGPDH
                     PDVIGDVYDLTLALARQGRLTEAFQLGDRLYSAWQRTAERPPAFDTLAALMVELGDAA
                     RSAGAPPEARKFFGIAVEIGRVTTPGDPQLATALWKLAVVILDAELATPNMANVTNVP
                     NMTNALSALQEALAIARAKTDTIEPEIHAAIEFDLARALWFTDPREAMTHAIAARTAF
                     VGLGEAGRAELGRVDAWLATHTPGR"
BASE COUNT          474 a         1212 c         1404 g          514 t
ORIGIN      
        1 cggcggcgca cgccggcggg gagtcggcga gggcgtcctc gcagccgatc gtgcaggcgc
       61 cggcgtccgc gctgaggcca cactgcgccg agtgctcgca catctgggcg cacacgctcg
      121 gctcgccgcc gtcggtgtcg ccggtggtga aagcgccggt ggtgaagtcg ccggtggtgt
      181 gatcctccac gccggtggtc gtctccggga tcacgacgct gtcgttcgag gtcgacggcg
      241 ggtccgtggt cgcgtcaggg ccgtcgccgg tcggcgccga gctgtggccg acggtggggt
      301 cggtatcggc ggcgctgctg ctgctgctag acccctcggg gtcgtccccg agcttgacgg
      361 tgcacgcgct cagcagggac gcgacgagtg agagcctggc gaagctggaa gtcatatcaa
      421 ggtctgcgct ggcacgacga tgcttgcaag cgcaggtccg atcggcgccc ggtcgcgacg
      481 agggccggct ccgcggcgca ggtccctgcg ccacaaagcc agccctcgct cccggtcgct
      541 actcctgcgc ccggatcttg atgtcgccct cgatcagcag attgtcgtag ctgtagttgg
      601 cgttggcgat cgcccgcggc ccgctcatcg cgctgacgcg cgcggtgccc tggaggaacc
      661 tgccgatgcg attggtgtcg atcgccgcca gcgacgtgcc agagccggcg aacgccgagt
      721 cgatgcatgc gccgacgttc tgctggtacg cacagtcgaa gatctcgatc accgtgacac
      781 tgtcctgagc gacgagcacg accctgggcg gccgcgccgg gttgacctgc tgctgctcgt
      841 tgaagtcggt ctcggggggc gggagcttgg cgtcggcggg ggcgggcgtc aggccaccgg
      901 cgagcatcac acataccagg gtcttcgaga atgagtgtaa gcgggtcatt gcatctccta
      961 tcgttcgcgc ggcttgaagc gttcacagct tcgaggaccg gtcgaaacac ctcgacggcc
     1021 gggcgaccac actgcacgcc atcgttcgcc gcgtcaacgc ttcaactgcg acggcccggc
     1081 ggcacgcgcc gcgtgtgccc agtgatcgtc gctatcgcgc gagcgtcgac ttcaacgacc
     1141 gggcgtgtgc gtcgccagcc acgcatcgac gcggccgagc tccgcgcgcc cggcctcgcc
     1201 gaggccgacg aacgctgtgc gagcggcgat cgcatgcgtc atggcctcgc gtggatcggt
     1261 gaaccacagg gcgcgggcca ggtcgaactc gatcgccgcg tggatctccg gctcgatcgt
     1321 gtcggtcttc gcgcgagcga tcgccagcgc ctcttgcaag gcgctcaggg cgttcgtcat
     1381 gttcggcacg tttgtcacgt tcgccatgtt cggtgtggcg agctcggcgt cgaggatcac
     1441 gacggcgagt ttccacagcg cggtcgcgag ctggggatca cccggtgtcg tgacgcgacc
     1501 gatctcgacg gcgatgccga agaacttccg ggcctccggg ggcgccccgg cggagcgggc
     1561 ggcgtcgccg agctcgacca tcagcgccgc gagcgtgtcg aaggccggcg ggcgctcggc
     1621 ggtccgctgc cacgcgctgt acaggcggtc gccgagctgg aacgcctcgg tgagccgacc
     1681 ctgacgcgcc agcgcgagcg tcaggtcgta gacgtcgccg atgacgtcgg ggtggtcggg
     1741 gccgagggca cgctcgcggg ccgccatcag gcgccgcagc accggctcgg cggcgccggg
     1801 cttgtcctgc cagatcagcg tctggccgag gctgctcagg gtggcgagcg tctcggggtg
     1861 ctcgggcccg agagctcgct cggccagcgc gaccgcccgg taggcgctct cgaccgcgag
     1921 cggccggctg gcaggatcgg cgttcgccga ggcgacgatg ttggtcagca gggcgacgag
     1981 ccgcggatgc tcgggcccga gcgaggtctc ccagatcgca agaccgcggc ggtagaacgc
     2041 gagcgcctcg gccgggcgac cctcggtgac ggcgacgttg ccgaggcccg acaggctcgg
     2101 gaccaggcgc gggtcatcgg ggccgaagct gcgctcggcg agcgcgagcg cctccgcgag
     2161 cgtcgcgcgg gcctcgggca aggcgccccg cgcgaagtgc acgttgccgg tgctgccgag
     2221 gaactggatc ctgacctccg ggtcctgatc gacgcgcgcg agcaggccgc cgatctgcgc
     2281 ggagaggtgc agcgcctcgt cgaagtgctt catgcggggc ccggtgagct ggatcagcgc
     2341 ggtgccgatc cgggcgcgca ggccgtcgtc gccggcggtc tcggcggcgg cgagggccgc
     2401 gaagccgctg gcctccgcgg ccacgtactc gcccatgcgc tcttgcacgc tcgcgcggcg
     2461 gtgcagcgcc atggcgatca cgggcgggta ggcgagcgcc tgcgcgtcgg tgacgacggc
     2521 gtcgatctgg gcgagggcgg cggggtagcg gccggcgctc tcctcggcct tggcccgggc
     2581 gacgcgctcg cgcaggtcgg ccgcggccgc ggcgacggtg ggcgtgggcg ggggggtctg
     2641 ggccatcagc agcgacgaat cgacgcagcg gtccagcgtc gagatcgcct cggtcgcctg
     2701 cacggcgcgc tcgatcgcgg tgcggtcggc ggtcttcaac acgtcgatca ccgcggcgac
     2761 ctcggcgagg cggtcgtcga ggcaggtcat gcggcgctcg aacagctcgt ccgactgctc
     2821 gccgcggtgg tgcgtgaggc aggcctcgcc gtgcatggcg gtccacgcct cggtgtaacg
     2881 atcgaggcgc gcggcgaggc ggacccaggt cgcgggggcg tacgacaggc cgctggcgag
     2941 cacggcgtgc tcgagggcgg cgcgggccgg tgggtcccac acgccgatca ggcgcgcggg
     3001 ggcgccggtg cacagctcgg cggacgcgct gcgctgcagg gcggcggcgc cgatcaggcc
     3061 gaccgcgagc gagacgaccg agagggcccc gagcgcggcg aaccagatcc ggcgcgggcg
     3121 atcgatcgcg gtgcgcaggc cggcgagcag ctcggccatc gacggcggtc gatccttgcg
     3181 gtccgccgcg aggccgcgca gcacgacctt gtgcacgcgg gcgggcacga tcgcgtcgcg
     3241 cgggagctcg gccatgtcgc cggcgacgat cgcctggcgc agctcgtcgg cgtcgtcgcc
     3301 ggtgaacggc cgctggccgt acagggcctc gaacaggctg acgcagaagc tgaagatgtc
     3361 gctgcgcgcg tcgatctcgc ccgagtcgag ctgctccggc ggcatgtagg cgggcgtgcc
     3421 gaccagcgag ccgaccatcg tgtgcgagac gtcgaacagg ccggacacct gcgaggcatt
     3481 ggcggcgttg aggtggctgc gctcggcgag cgactcgagc ttgaagtcgc tgcgccgggc
     3541 gaggccgaag tcgacgacgc gcacgcggcc gtccttgccg acgaggatgt tgtcgggctt
     3601 gagg
//
LOCUS       DASWNJ010000378         1600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_183605, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000378 DASWNJ010000000
VERSION     DASWNJ010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183605"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VGB85_06595"
     CDS             complement(<1..472)
                     /locus_tag="VGB85_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133728.1"
                     /translation="MLTHVLLAIAMIAPAPAFTTTETGPRGLWSDGDGLLAVTGYDKV
                     ELSCRTGQRITVHDVKGLRDVDGAWVDGAPVVAAVGEARELVLSRGGRWSLRAVPAGT
                     DEKTIAVAVDPRGKVVVLGETQAVYRLDGERWDVLRFPAGSARAVALAVRADGDI"
     gene            complement(513..>1600)
                     /locus_tag="VGB85_06600"
     CDS             complement(513..>1600)
                     /locus_tag="VGB85_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0133729.1"
                     /translation="NELVAGVRSDVGVILYGPDLRVLVSESARVLDLVRGIAGVVDLR
                     AEQLEGLQYLRIVPDRVKLARFGLTVADINLATEAISVGHEAGVILEGERRFDMMVKV
                     DHGYAGDLRALASLPLRAQTGQLVPLGDVASLSFQPGPALVNREDQSRRLVVEFNVRG
                     RDLVSVVEQVQATLRDGLTLPTGYRVEYGGTFEHYLAARDRLMIVVPLALVLILFLLW
                     TALGRVRAAIIIFLNVPFAIVGGVLALWLRDLPFSISAGVGFIALFGVAVLNGLVLVS
                     FAQRLEERGMAHELAIREAAELRLRPVLTTALVASLGFVPMALSTDPGSEVQRPLATV
                     VIGGLVTASLLTLLVLPVVYAIFGRRR"
BASE COUNT          265 a          578 c          576 g          181 t
ORIGIN      
        1 agatgtcgcc gtccgcacgc accgcgaggg cgaccgcgcg ggcggacccg gccgggaagc
       61 ggagcacgtc ccagcgctcg ccgtcgaggc gatacaccgc ctgcgtctcg ccgagcacga
      121 cgaccttgcc gcgcgggtcg acggcgacgg cgatcgtctt ctcgtcggtg cccgccggca
      181 ccgcccgcag cgaccaccgc ccgccgcgcg acagcacgag ctcgcgcgcc tcgccgacgg
      241 cggcgacgac cggcgccccg tcgacccacg cgccgtcgac gtcacgcagg cccttcacgt
      301 catggaccgt gatgcgttga cctgtccgac aggacagctc gaccttatcg taccccgtca
      361 ccgcgagcag gccgtcgccg tcgctccaca ggccgcgagg tccggtctcc gtcgtcgtga
      421 acgcgggcgc gggcgcgatc atcgcgatcg ccagcagaac gtgggtcagc accggcgcag
      481 catactcgtc ggaccgcccg gcagcgaagc ttctaacggc gccggccgaa gatcgcgtag
      541 acgaccggca gcacgagcag cgtcagcagg ctcgcggtga cgaggccgcc gatcacgacg
      601 gtggcgagcg ggcgctggac ctcgctgccg gggtcggtcg acagcgccat cggcacgaag
      661 ccgagcgagg cgaccagcgc ggtggtcagc accgggcgca ggcgtaattc ggcggcctcg
      721 cggatcgcca gctcgtgggc catgccgcgc tcctcgaggc gctgggcgaa cgacaccagc
      781 acgaggccgt ttagcaccgc gacgccgaac agggcgatga agccgacgcc ggcggagatc
      841 gagaagggca ggtcgcgcag ccacagcgcc agcacgccgc cgacgatcgc aaagggcacg
      901 ttcaaaaaga tgatgatcgc cgcgcgcacg cgcccgagcg cggtccacag caggaacagg
      961 atcaacacca gcgcgagcgg cacgacgatc atcaggcggt cgcgggccgc gaggtagtgc
     1021 tcgaaggtgc cgccgtactc gacgcggtag ccggtcggca gcgtcaggcc gtcgcgcagg
     1081 gtcgcctgca cctgctcgac gaccgacacg aggtcgcgcc cgcggacgtt gaactcgacg
     1141 acgagccggc gcgactggtc ctcgcggttg accagcgccg ggccgggctg gaagctcagc
     1201 gaggcgacgt cgccgagcgg caccaactgg ccggtctggg cgcgcagagg caggctggcg
     1261 agcgcgcgca ggtcaccggc gtagccgtgg tcgaccttga ccatcatgtc gaagcgccgc
     1321 tcgccctcga ggatcacgcc ggcctcgtga ccgacgctga tcgcctcggt ggcgaggttg
     1381 atgtcggcga cggtcagtcc gaagcgcgcc agcttgaccc ggtccggcac gatccgcagg
     1441 tactggaggc cctcgagctg ctcggcgcgc aggtcgacga cgccggcgat cccgcgcacg
     1501 aggtcgagca cgcgcgcgct ctcggacacg aggaccctga ggtcgggccc gtagaggatc
     1561 acgccgacgt cggatcgcac gccggcgacc agctcgttgg
//
LOCUS       DASWNJ010000379         3844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_394256, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000379 DASWNJ010000000
VERSION     DASWNJ010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394256"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VGB85_06605"
     CDS             complement(<1..246)
                     /locus_tag="VGB85_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133730.1"
                     /translation="MPERPTTPATEIVHPVAVSLDPDEIPDLPRTRFTSETTYNPELR
                     RRRRTIYTVITAALSLIIITSIYQIQSLGGSDESGDLH"
     gene            complement(378..1499)
                     /locus_tag="VGB85_06610"
     CDS             complement(378..1499)
                     /locus_tag="VGB85_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133731.1"
                     /translation="MYSSREPGTRTSNRNADSTVVGRSASLGRPESSSAAAPSDARAS
                     RHMVGVSAFAACALGLTFAVFLQLALLMMSRQGQLVAGSSRAECEEGDALSCPNGTTC
                     SGGSCVAVPALDTCQVGDSCDMTGASCRCQAPLRCQANVCTANVITASCDDPIVHKVL
                     VEVQKTCQGSWNNCPEDKLKDFVLNSADFDQVIAAVPGTMTVHFPGGRPFLSHGKDWP
                     SDAELAHYRKQLSTPAVAKALSEAQDLLLIGRSSHSQNPEEDLRYSRARVNTVTELLT
                     SSAPGPSESTALRGKFRQLMLGSRKVLQPDVFAKNYANRIVAWNEDIERYLRSKLVDV
                     TKLSAKERKGILGMVNQVVFVVPIPCKLPVDGTIAGAVR"
     gene            complement(1504..2835)
                     /locus_tag="VGB85_06615"
     CDS             complement(1504..2835)
                     /locus_tag="VGB85_06615"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEY0133732.1"
                     /translation="MGDKRPQKPERQDLLRQLNHDVGACALLCLLSIPGVVLSVFLTL
                     VKFRSDYRCDYAMLSACSIGQWFDCNHVLSSDASIVLKLPISAYSTGYYLAVFGLAVA
                     ALWRPRMLPVLRPLLLWLGWIGLLVVLGLASYTVFVLREGCSYCFIIYSLTAAIFLTT
                     ALMHPQGHWQGLRALLVPWRPRQGGVLLLTGLAFLALISVQMVQYRRAANIVFDSKCL
                     VSNMVLPETHLRTQAPRITAQISLFVDFSCHHCRREFEFWRDFVAANPDEYVFEVYHF
                     ARDGECMREADRGFSNQATRNYSCLAAQAAECAEKLRPGTGMAMVSALFNLQDRGSPP
                     YFTELSIGEAASSVGFEAIPKENFEHPFYTCVREDKQVITHIAGHTRYILENGIVDPP
                     VTYITSYDADGQALPRLVQSQGAKQHASPLKTLQVARKAAMPGDSSAAPAE"
     gene            complement(2845..>3844)
                     /locus_tag="VGB85_06620"
     CDS             complement(2845..>3844)
                     /locus_tag="VGB85_06620"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY0133733.1"
                     /translation="MALPLVDHFGELYGRSAAMRGLFARFAHMAERGDQSHVLVRGKP
                     GTGRQLVARVLHAGSARSRRPFVVLDCASVAPELLASHLFGRGCSDAPGRFDVCGGLE
                     AAHGGTLLLKAVDSLPLALQGQLLAVLQRGVLTREGEQHARKFDARLICATDRDIQRL
                     VAEGKFNQELYRHLSGVQVDLPALCDRDDDVAFLASEVFLKRLCDGTGRTRRLAPEAL
                     DALRAHAWPQNLSELRCAVERGFYAREDEWIRPADLGLSLGVTFHDGLLRKLVEIEAL
                     FANNHSGAVAGFERLYFSHLLLSHPSKARAARFASMTNEGFRLALKRLGLGARCAG"
BASE COUNT          650 a         1210 c         1383 g          601 t
ORIGIN      
        1 gtggaggtcg ccggactcgt cggagccgcc gagcgactgg atctggtaga tgctcgtgat
       61 gatgatgaga gacagcgccg cagtgatgac ggtgtagatc gtcctgcgcc gtcggcgaag
      121 ctcgggatta taagtcgtct cgctggtaaa ccgcgtgcgc ggcagatccg ggatctcatc
      181 gggatcgagc gagactgcga cggggtgtac gatctccgtg gcaggcgtgg tgggccgctc
      241 gggcatccgg gcgatctgag tctctgcggc atgcgatggc ggcggcgcca cggcgcggtc
      301 ggtcgatccc tcgacgatct cgaccgggac ctctggcacg gtcgtgtggg actccccgtc
      361 cgagggctgg cgcgaggtca ccgcaccgct cccgcgatgg tgccgtcgac gggcagctta
      421 caggggattg ggaccacgaa cacgacttga ttcaccatac cgaggatccc cttgcgctcc
      481 ttggcgctga gcttggtgac gtcgacgagc ttcgagcgca ggtatcgctc gatgtcctcg
      541 ttccacgcga cgatgcggtt ggcgtaattc ttggcgaaga cgtcgggctg caggaccttg
      601 cggctgccga gcatcagctg acgaaacttg cctcgcaggg cggtgctctc gctgggtccc
      661 ggcgcggagc tggtcagcag ctccgtcacc gtgttgacgc gagcgcgcga atacctgagg
      721 tcttcctcgg ggttttggct gtggctcgag cgcccgatca gcaggagatc ctgggcctca
      781 ctcagcgcct tggcgaccgc cggtgtcgat agttgcttgc ggtagtgcgc cagctcggcg
      841 tcgctcggcc agtcctttcc gtgagagagg aacggccgcc cgcccgggaa gtggaccgtc
      901 atcgtgcccg ggaccgcggc gatgacctgg tcgaagtctg ccgagttgag gacgaagtcc
      961 ttgagcttgt cctcggggca gttgttccag gaaccctggc aggtcttctg gacctcgacg
     1021 agcaccttat gcacgatcgg gtcgtcgcag ctcgccgtga tgacgttggc ggtgcacacg
     1081 ttcgcctgac agcgcagcgg cgcctggcag cggcagctcg cgccggtcat gtcgcaggag
     1141 tcgccgacct ggcaggtgtc gagcgccggg acggcgacgc agctaccacc cgagcaggtc
     1201 gtgccattcg gacagctaag cgcgtcgccc tcctcgcact ctgcgcggga gctcccggcg
     1261 acgagctggc cctgcctgct catcatgagc agggccagct gcaggaacac ggcgaaggtc
     1321 agtccgagcg cgcacgccgc gaaggcgctg acaccgacca tatgtcggga ggcgcgggcg
     1381 tccgacgggg ccgccgcgct cgaggactcg gggcggccca gcgaggcgct gcggccgacg
     1441 accgtggagt cggcgttgcg attggacgtg cgggttccgg gttcacgtga agaatacatg
     1501 ggttcattcc gccggagcgg ccgacgagtc gccgggcatg gcggcctttc gtgcgacctg
     1561 gagggttttc agcgggctgg catgctgctt cgcgccctgg ctctgcacga gcctcggaag
     1621 cgcctggccg tcggcgtcgt aagaggtgat gtatgtgacc ggcgggtcga cgatgccgtt
     1681 ctcgaggatg taacgggtgt gaccggcgat atgcgtgatg acctgcttgt cctcgcgcac
     1741 gcaggtgtaa aaggggtgct cgaagttctc cttggggatc gcctcgaagc cgaccgagct
     1801 cgccgcctcg ccgatgctga gctctgtgaa gtacgggggc gagccgcggt cctggagatt
     1861 gaagagcgcg gagaccatcg ccatgccggt gccgggtcgc agcttctcgg cgcactcggc
     1921 ggcctgcgcg gcgaggcagg agtagtttcg ggtcgcctgg ttgctgaatc cgcggtcggc
     1981 ctcacgcatg cactcgccgt cgcgggcgaa gtgatagacc tcgaacacgt actcgtcggg
     2041 gttggcggcg acgaagtcgc gccagaactc gaactcacgc cggcagtgat ggcacgaaaa
     2101 gtcgacgaag agcgagatct gggcggtgat gcgcggggcc tgcgtgcgca ggtgggtctc
     2161 gggcaacacc atattggaca ccaggcactt cgaatcgaac acgatgttcg cggcgcggcg
     2221 atattgcacc atctgcaccg agatcagcgc caggaacgcg agcccggtca gcagcaggac
     2281 gccaccctgg cgcggtcgcc agggcacgag caaggcacgc aggccctgcc agtggccttg
     2341 tgggtgcatg agggcggtcg tcaggaagat cgccgcggtc aggctgtaaa tgatgaagca
     2401 atagctgcaa ccctcgcgca ggacgaacac cgtgtaactg gcgagcccga gcacgacgag
     2461 caggccgatc cagccgagcc agagcagcag cgggcgcagc accggcaaca tgcgcgggcg
     2521 ccacagcgcc gccacggcga gcccgaacac ggcgaggtag taaccggtcg agtacgccga
     2581 gatcggcagc ttgaggacga tcgaggcgtc gctgctgagc acgtggttgc agtcgaacca
     2641 ctgaccgatc gagcacgccg agagcatcgc atagtcgcag cgatagtcgg agcggaactt
     2701 gacgagcgtc aggaatacgc tgaggaccac gcccgggatg ctgagcagac acagcagcgc
     2761 gcacgcgccg acatcatggt tcaactggcg cagcagatcc tgccgctccg gcttttgtgg
     2821 ccttttgtcg cccataaagc cacctcagcc ggcgcagcgg gccccgaggc cgaggcgctt
     2881 gagggccagc cggaagccct cgttcgtcat gctggcgaag cgggcggccc tcgctttgct
     2941 cgggtgactg agcagcaggt gcgagaagta caggcgctcg aagccggcga ccgcgccgct
     3001 gtggttgttg gcgaacaacg cctcgatctc gaccagcttg cgcaggagcc cgtcatggaa
     3061 ggtgacgccg aggctgagcc cgagatcggc gggacggatc cactcgtcct cgcgcgcata
     3121 aaagccgcgc tcgaccgcgc agcgcagctc ggagaggttc tgcggccagg cgtgcgcgcg
     3181 cagggcgtcg agcgcctcgg gtgcgagccg gcgcgtccgc ccggtgccat cgcagagccg
     3241 cttgaggaac acctcggagg cgaggaacgc gacgtcgtcg tcgcggtcgc acagggccgg
     3301 tagatcgacc tgcacgccag acaggtgacg gtagagctcc tgattgaact tgccctccgc
     3361 gacgagacgc tggatgtcgc ggtcggtcgc gcagatcaga cgggcgtcga acttgcgcgc
     3421 gtgctgctcg ccctcacggg tcaggacgcc ccgctgcagc accgcgagca gttgaccctg
     3481 caacgccagc ggcaggctgt cgacggcctt gagcaggagg gtcccgccgt gggccgcctc
     3541 gaggccgccg cacacgtcga agcggccagg agcgtccgag cagccgcgac cgaacaggtg
     3601 gctcgccagg agctccggcg cgacgctggc gcaatcgagg acgacgaatg ggcgcctgct
     3661 acgcgccgag ccggcgtgca acacccgggc gaccagttgc cgacccgtac cgggcttgcc
     3721 gcggacgagc acgtgggatt gatcgccgcg ctcggccatg tgcgcgaagc gggcgaacag
     3781 gccgcgcatc gccgcgctgc gcccgtagag ctcaccgaag tgatcgacca gcggcaaggc
     3841 catg
//
LOCUS       DASWNJ010000380         3307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_42466, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000380 DASWNJ010000000
VERSION     DASWNJ010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42466"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VGB85_06625"
     CDS             <1..697
                     /locus_tag="VGB85_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133734.1"
                     /translation="ADPEVQALGGPRVVLLASLAPFLADEAWQRRHAAALASRLTPPA
                     LPGLSDMLRRPSGIAGLGLVAVTLLLLLRPDPPASPPAPPPPPVAVAVPLPPPVVVAP
                     PLPLPVPAAISPAPAPVRKAARRGSRRARPDEALAAREKLAAARDPGAIIVELEMIGA
                     GRKSLASNPRQALAYADQHARDYPDSQLRAQRAELRVRALCALGRRDEARAEAQRNQQ
                     SKVQDALQTACKP"
     gene            complement(716..2908)
                     /gene="rlmKL"
                     /locus_tag="VGB85_06630"
     CDS             complement(716..2908)
                     /gene="rlmKL"
                     /locus_tag="VGB85_06630"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: protein motif:HMM:NF008748.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HEY0133735.1"
                     /translation="MSATPEDHPFFATCPRGLEELLAAELTTLGAAAIKLQPAGVLFQ
                     GSLQLGYRVCLWSRLAGRVLLELVRGPASDGDELYRTALGVDWSAHMEGTQTLAVDFT
                     GGNAAIVHTTFGAQRVKDAVVDQLRVGDRRPSVDKLRPDLRIHVHLRKRTGPPGQAPE
                     SARDEAVISIDLSGESLHRRGYREEGAMAPLKENLAAAILVRGGWPAIAAAGGPLLDP
                     MCGSGTLLIEGAWIAGDRAPGLGRDYFGFLGWRQHEPAIWGELVAEASGRAAAGRDKI
                     PDIRGYDTSGLAIAAAHRNIEAAGLRTQVHVERWAIGEQLLAPVPADRPPGLVVCNPP
                     YGARVGEGEDLRPLYRELGELLKTRHMGGDAAILLEDRELGHAIGLRAKKIYDLYNGA
                     LPVHLLRFKIEEAAIQRPRSERPVVRSEAAASFANRLEKNRKQLAPWAAQEGIDCYRV
                     YDSDIPEYAVAVDIYGEHAHVQEYAAPKEIEPEKAEARLRDVMAVVPEVLGIPREQVH
                     LKVRLKSRGGSQYGKQAAAGKLLMVREGKPRFFVNLTDYLDTGLFLDHRKTRVRVGEL
                     ARGRRFLNLFAYTGSATVHAVLGGAIASTTVDMSPTYTAWARKNFDLNNITGPEHRIV
                     TAECRDWMREEHRRYGLIFLDPPTFSNSKRMAGEFDLQRDHVELIREAAHLLERGGIL
                     LFSTNFRRFRMDSEALADLTITDITRDTIPRDFARNPRIHTCWQIERR"
     gene            complement(2954..>3307)
                     /locus_tag="VGB85_06635"
     CDS             complement(2954..>3307)
                     /locus_tag="VGB85_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY0133736.1"
                     /translation="AAAVGRPLELIHVGQAHDGDMLGALDPRWKIEHEGHRAEVLAAV
                     NAWTFEHSLGDCPRRVLFGEPVAEIAGAAAELHAALVVVGSRQLGVGARIFTTSTASA
                     LAGVAGCAVAVVPPA"
BASE COUNT          415 a         1265 c         1171 g          456 t
ORIGIN      
        1 ggccgacccc gaggtgcagg cgctcggcgg cccgcgcgtc gtgctgctgg ccagcctcgc
       61 gcccttcctc gccgacgagg cctggcagcg ccgccacgcc gccgcgctcg cctcgcgcct
      121 gacgccgccg gccttgcctg gcctctcgga catgctgcgc aggccctccg ggatcgccgg
      181 cctcggcctc gtcgcggtga cactgctgct gttgttgcga cccgacccgc cggcgtcgcc
      241 ccctgccccg cccccgccgc cggtcgccgt cgcggtgccc ttgccaccgc cggtggtggt
      301 cgccccgccg ttgccattac ctgtcccggc ggccatttcc cccgcgccag ccccggtccg
      361 caaggccgcg cgccgcgggt cccggcgcgc gcgcccggac gaggccctcg ccgcgcgcga
      421 gaagctggcc gccgcgcgcg accccggggc gatcatcgtc gagctcgaga tgatcggcgc
      481 cggccgcaaa tcgctcgcca gcaatccccg ccaggcgctc gcctacgccg accagcacgc
      541 ccgcgactac cccgacagcc agctgcgggc gcagcgcgcc gagctgcgcg tgcgggccct
      601 gtgcgcgctc ggccgccgcg acgaggccag ggcggaagcc cagcgtaacc agcagagcaa
      661 ggtccaggac gcgttgcaga ccgcctgcaa accatgaacc cggctcacgc cccgctcatc
      721 ggcgttcgat ctgccagcac gtgtggatcc gcgggttgcg ggcgaagtcc cgcgggatcg
      781 tgtcacgcgt gatgtcggtg atcgtcaggt ccgcgagcgc ctcgctgtcc atgcgaaagc
      841 ggcgaaagtt ggtcgagaac agcaggatcc cgccgcgctc gagcaggtgg gccgcctcgc
      901 ggatcagctc gacgtggtcg cgctgcaggt cgaactcgcc ggccatccgc ttcgagttcg
      961 agaaggtcgg cgggtcgagg aagatcagcc cgtagcggcg atgctcctcg cgcatccagt
     1021 cgcggcactc ggcggtgacg atgcggtgct cgggcccggt gatgttgttg aggtcgaagt
     1081 tcttgcgcgc ccacgcggta taggtcggcg acatgtcgac cgtcgtcgac gcgatcgcgc
     1141 cgccgagcac cgcgtgaacc gtcgccgagc cggtgtacgc gaacaggttg aggaaccggc
     1201 gcccgcgcgc cagctcgccg acccgcacgc gggtcttgcg atgatcgagg aacagcccgg
     1261 tgtcgaggta gtccgtcagg ttgacgaaga agcgcggctt gccctcgcgg accatcagca
     1321 gcttgcccgc cgccgcctgc ttgccgtact gcgagccgcc ccggctcttg agtcgcacct
     1381 tgaggtggac ctgctcgcgc gggatcccga gcacctcggg gacgaccgcc atcacgtcgc
     1441 gcaggcgcgc ctcggccttc tccggctcga tctccttcgg cgccgcgtac tcctgcacgt
     1501 gcgcgtgctc gccgtagatg tcgaccgcga ccgcgtactc cgggatgtcc gagtcgtaca
     1561 cgcgatagca gtcgatgccc tcctgcgcgg cccacggcgc cagctgcttg cggttcttct
     1621 cgagccggtt ggcgaacgac gccgccgcct cgctgcgcac gaccggccgc tcgctgcgcg
     1681 gccgctggat cgccgcctcc tcgatcttga agcgcagcag gtgcaccggc agcgccccgt
     1741 tgtagaggtc gtagatcttc ttcgcgcgca ggccgatcgc gtggccgagc tcgcggtcct
     1801 cgagcaggat cgccgcgtcg ccgcccatgt gtcgcgtctt gagcagctcg ccgagctcgc
     1861 ggtacagcgg gcgcaggtcc tcgccctcgc cgacgcgcgc gccgtacggc gggttgcaca
     1921 cgacgagccc cggcggcctg tccgcgggga caggtgcgag cagctgctcg ccgatcgccc
     1981 agcgctcgac gtggacctgc gtgcgcagcc cggcggcctc gatgttgcga tgcgccgccg
     2041 cgatcgccag gcccgaggtg tcgtagccgc ggatgtcggg gatcttgtcg cggccggccg
     2101 ccgcgcgccc gctcgcctcg gcgaccagct cgccccagat cgcgggctcg tgctggcgcc
     2161 agccgaggaa gccgaagtag tcgcgaccga ggcccggcgc gcggtcgcct gcgatccacg
     2221 cgccctcgat cagcagcgtg cccgagccgc acatcgggtc gagcagcggc ccgcccgcgg
     2281 ccgcgatcgc cggccagccg ccgcgcacca ggatcgcggc cgcgaggttc tccttgagcg
     2341 gcgccatcgc gccctcctcg cggtagccgc ggcggtgcag gctctcgccg gacaggtcga
     2401 tcgagatcac ggcctcgtcg cgcgcgctct ccggcgcctg tcctggagga cctgtccgtt
     2461 tgcgcaggtg cacgtggatc cgcagatcgg gccgcagctt gtcgaccgag gggcggcgat
     2521 cgccgacgcg cagctggtcg acgaccgcgt ccttgacccg ctgggccccg aaggtggtgt
     2581 gcacgatcgc cgcgttgccg ccggtgaagt cgaccgcaag ggtctgtgtg ccctccatgt
     2641 gcgccgacca gtcgacgccg agcgccgtcc ggtacagttc gtcgccgtcg ctcgccgggc
     2701 cgcgcacgag ctcgagcagc acgcgtccgg ccagccgcga ccacaggcac acgcgatagc
     2761 cgagctgcag cgatccctgg aacagaacgc cggcgggctg aagtttgatc gccgcggcgc
     2821 cgagcgtcgt cagctcggcg gcgagcagct cctcgagtcc ccgggggcag gtcgcgaaga
     2881 acgggtgatc ctcgggcgtc gcggacatcg cggcctctta gcacggcgcg cgggcgcccg
     2941 cccgccctcg ctgtcacgcc ggcgggacca cggcgacggc gcagccggcc acccccgcga
     3001 gcgcggacgc cgtcgaggtc gtgaagatgc gggccccgac gccgagctgg cgcgagccga
     3061 cgaccaccag ggccgcgtgg agctccgcgg cggcgcccgc gatctccgcg accggctcgc
     3121 cgaacagcac gcggcgcggg cagtcgccga gcgagtgctc gaaggtccac gcgttgacgg
     3181 ccgccagcac ctccgcgcgg tggccctcgt gctcgatctt ccagcggggg tcgagcgcgc
     3241 cgagcatgtc gccgtcgtgc gcctggccga cgtggatcag ctcgagcggc cggccgaccg
     3301 cggcggc
//
LOCUS       DASWNJ010000381         3922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_359382, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000381 DASWNJ010000000
VERSION     DASWNJ010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359382"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3922
                     /locus_tag="VGB85_06640"
     CDS             <1..>3922
                     /locus_tag="VGB85_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEY0133737.1"
                     /translation="EVTTTGEQMHLTAPVLALFRRLPQCTLHNHYGPSESHVVTAYTL
                     PGDPDLWVPHPPIGGAIDNTRLLVLDASMNLCPVGVPGELYIGGLCLARGYWNRPALT
                     AERFISDPHSDDPTARLYRSGDIARWRPDGTIEYMGRRDDQVKIRGVRVEIGEVESSI
                     SRHPDVLETIVLAREDRPGERRLVAYVVMRSAGTGLAELRAFLRQSLPEVMIPAAIVP
                     MSALPLTGNGKIDRRALPAPEDVTTPRTTPLVAPRSEIEAALASIWREVLRVDRLGVH
                     DDFFELGGHSLAATQVFSRVRRIFGVELSFARLFTLPTIAALAAWIRAERASGPDAGD
                     TIARAPRDRPVPLSSAQERLWFLHKMAPGSVSYHCPYFFRVGGPLDVAALQRAFDGLF
                     ARHEVLRTTFHEAGGRPLQVISRTGTITLERIDLTLLAPDDRAAQLRSCLDREGLRPF
                     DLERGPLLRAGLVRVTETEHVLWLNMHHIILDGWSMDVLLRELAALYTGDRSLPEPAL
                     QYADYAIWERARSERDLSQAVAWWRAKLAGAPALLALPGDRPRPPVPSSRGGSVRFHL
                     EKSQGEALRALGVKRGLTLAMVMFAGYVALLHRYTGEHDLVIGIPTSGRDRVELEPLI
                     GLFVNTLPVRVDLSGDPSFLELLDQVRAVCLEAYDHDNVPFDRLVQELAPQRSIGHNP
                     LVQIGFAPQPAGERDLRLAGLEVEALEIDAAKTVFDLALYTWEDSASITGLLEYSVDL
                     FDRETIDRMRDHLVALLCGAVAEPDRKVSSIALAPVDKAGPRAATGPVAHELMHTLFE
                     QHAARNPGHPAIVLGDARMSHAMLDERADLLARHLRARGVGAETLVALSVERSFDLIV
                     GVLAILKAGGAFVPLDPEYPADRLAFMLEDSAAPVVLTQAKLADNFKQHRCVIRLDAD
                     WAEIARAPGPVPRDVARPDNAAYVIYTSGSTGRPKGVVVEHRNAVNLAHAQRAMYGLT
                     AASVVLQFASLSFDAFVSELLSAWAVGATLCLLPPGLAVPGREFTDILRRQQVSLFAL
                     PPSLLAVLPVESLPALTHVVSGGEPCSAEVVDRWAPGRTFLNVYGPTEATVCASFARC
                     QPGAGVPPIGGPLANVQLYVLDAGGHPAPVGVPGELYIGGAGVARGYLNRPELTHERF
                     VADPFVGEAGARMYRTGDRVRVRGDGQLEFLGRLDQQIKLRGFRVELGEVEAALRAHP
                     GVQDAAVIVGTDLVAFVVPGAVVGSTDAQDEHVDAWRAVNDETYAGPTVGEATLDITG
                     WTSSHTGLPIPAEVMRVWRDSTVDRIAALPIGPARRVWEIGCGSG"
BASE COUNT          515 a         1387 c         1398 g          622 t
ORIGIN      
        1 gggaagtgac gacgacgggc gagcagatgc acctgaccgc gccagtgctg gcgctgtttc
       61 gccggctgcc gcagtgcacg ctgcacaacc actacggccc gtccgagagc cacgtggtca
      121 cggcctacac cttgccgggc gacccggacc tgtgggtccc gcacccgccg atcggcggcg
      181 cgatcgacaa tacgcggttg ctcgtactcg acgcgagcat gaacctgtgc ccggtcggtg
      241 tcccgggcga gctctacatc ggcggcctgt gcctggcccg cggctactgg aaccgcccgg
      301 cgctgacggc cgagcgcttc atctccgatc cgcactcgga cgacccgacg gcccgcctgt
      361 atcgcagcgg agatatcgcg cgctggcgcc ctgacggaac gatcgagtac atgggccggc
      421 gtgacgacca ggtcaagatc cgcggcgtgc gggtcgagat cggcgaggtc gagtcctcga
      481 tcagccgcca cccggacgtg ctcgagacga tcgtgctcgc gcgcgaggac cggcccggcg
      541 agcgtcgcct cgtcgcgtat gtcgtgatgc gatccgccgg caccgggctc gccgagctgc
      601 gcgccttcct gcgtcagagc ctgcccgagg tgatgatccc ggcggcgatc gtgccgatgt
      661 cggcgctgcc gctgaccggc aacggcaaga tcgaccggcg cgcgctgccg gcccccgagg
      721 atgtcacgac cccgcggacc accccgctgg tcgcgccgcg ctcggagatc gaggcggcgc
      781 tcgcttcgat ctggcgcgag gtgctgcgcg tcgatcggct cggcgtgcac gacgacttct
      841 tcgagctcgg cggccactcg ctcgccgcga cccaggtgtt ctcgcgggtg cgccggatct
      901 tcggcgtcga gctctcgttt gcgcggctgt tcacgctgcc gacgatcgcc gcgctcgcgg
      961 cgtggatccg ggccgagcgg gcctccgggc ccgatgccgg cgatacgatc gcccgggccc
     1021 cgcgcgatcg gccggtcccg ctgtcctcgg cccaggagcg cctgtggttc ctgcacaaga
     1081 tggcgcccgg gagcgtgtcc tatcattgtc cgtacttctt tcgcgtcggc ggaccgctcg
     1141 acgtcgcggc gctgcagcgc gcgttcgacg ggctgttcgc gcgtcacgag gtgctgcgga
     1201 ccaccttcca cgaggccggc ggacggccgc tgcaggtgat ctcgcggacc ggcacgatca
     1261 cgctcgagcg catcgacctc acgctgctcg ccccggacga tcgcgccgcg cagctgcgtt
     1321 cgtgcctcga ccgcgagggc ctgcgcccct tcgacctcga gcgcgggccg ctgctgcgcg
     1381 ccggcctggt gcgtgtcacg gagaccgagc acgtgctgtg gctcaacatg catcacatca
     1441 tcctcgacgg ctggtcgatg gacgtgctgc tgcgcgagct ggccgcgctt tataccggcg
     1501 atcgttcgct gccggagccg gcgctgcaat atgcagacta tgcgatctgg gagcgcgcga
     1561 gatccgagcg cgacctgtcg caggcggtcg cgtggtggcg ggccaagctg gccggcgcac
     1621 cggccttgct ggcgttgccc ggggaccgcc cgcgcccgcc ggtgccgagc tcgcgcggcg
     1681 ggtcggtgcg ctttcacctg gaaaaatcac agggcgaggc gctgcgggcc ctcggcgtca
     1741 agcgcggcct gacgctggcg atggtgatgt tcgccggata cgtcgcgctg ctgcatcgct
     1801 acaccggcga gcacgacctc gtgatcggca tcccgacctc gggccgcgat cgcgtcgagc
     1861 tcgagccgct gatcggcctg tttgtcaata ccttgccggt gcgcgtcgac ctttcgggcg
     1921 acccgtcgtt cctcgaattg ctcgatcagg tgcgcgcggt ctgcctcgag gcgtacgacc
     1981 acgacaacgt gcccttcgat cgcctggtgc aggagctcgc gccgcagcgc agcatcggcc
     2041 acaacccgct ggtccagatc ggcttcgcgc cgcagcctgc cggtgagcgt gacctgcggc
     2101 tcgccggact cgaggtcgag gcgctcgaga tcgacgccgc caagacggtc ttcgacctcg
     2161 ctctttatac ctgggaggac agcgccagca tcacggggct cctcgaatac agcgtcgacc
     2221 tgttcgaccg cgagacgatc gatcgcatgc gcgaccatct ggtcgcgttg ctgtgcggcg
     2281 cggtcgccga gccggatcgc aaggtctcga gcatcgcgct cgcgccggtc gacaaggccg
     2341 ggccgcgcgc ggcgaccggg ccggtcgcgc acgagctgat gcacacgctg ttcgagcagc
     2401 acgccgcccg caacccgggg catcctgcga tcgtgctcgg tgacgcgcgc atgagccacg
     2461 cgatgctcga cgagcgcgcc gacctgctcg cgcggcacct gcgggcgcgg ggcgtcggcg
     2521 cggagaccct cgtcgcgctg agcgtcgagc ggtcgttcga cctcatcgtc ggtgtgctgg
     2581 cgatcctcaa ggcgggcggg gcgttcgtgc cgctcgaccc cgagtatccc gccgaccggc
     2641 tggcgttcat gctcgaggac agcgcggccc cggtcgtgct cacgcaagca aagctcgccg
     2701 acaattttaa gcagcaccgg tgcgtgatcc ggctcgacgc ggattgggcc gagatcgccc
     2761 gcgcgcccgg gccggtgccg cgagatgtcg cccgccccga caacgccgcc tacgtaattt
     2821 atacctcggg gtcgaccggg cgccccaagg gcgtggtcgt cgagcaccgc aatgcggtca
     2881 acctcgctca cgcccagcgc gcgatgtatg gattgacggc ggcgagcgtg gtgctgcaat
     2941 tcgcgtcgct gagcttcgat gcgttcgtgt ccgagctgct cagcgcgtgg gccgtcggcg
     3001 cgaccctgtg cctgctcccg cccggcctgg cggtccccgg ccgtgaattc accgacatcc
     3061 tgcgcaggca gcaggtgtcc ctgttcgcgc tgccgccctc gctgctcgcg gtgctgccgg
     3121 tcgagtctct ccccgcgctg acgcacgtcg tctccggcgg cgagccatgc agcgccgagg
     3181 tcgtcgatcg ctgggcaccc ggtcggacct ttttaaatgt ctacgggccg accgaggcga
     3241 ccgtctgcgc gagcttcgct cgctgccagc ccggggcagg ggtcccgccg atcggcggac
     3301 cgctggcgaa tgtgcagctg tatgtgctcg acgcgggtgg acatcccgcg cccgtcgggg
     3361 tcccgggcga gctgtacatc ggcggcgccg gtgtggcgcg tggatacctc aaccgccccg
     3421 agctgacgca cgagagattt gtcgccgacc cgttcgtggg ggaggcgggc gcacgcatgt
     3481 atcgcaccgg cgatcgtgtg cgcgtgcgcg gcgacggcca gctcgagttt ctcggtcgac
     3541 tcgatcagca gatcaagctg cgcgggtttc gggtcgagct cggcgaggtc gaggcggccc
     3601 tgcgggcgca cccgggcgtg caggacgccg cggtgatcgt cggcacggat ctcgtcgcgt
     3661 tcgtcgtccc gggcgcggtg gtcggctcga ccgacgcgca ggacgagcac gtcgacgcct
     3721 ggcgcgcggt caacgacgag acctacgcgg gtccgaccgt gggagaggcg acgctcgaca
     3781 tcacgggctg gacgagcagc cacaccggcc tgccgatccc cgcggaggtg atgcgggtgt
     3841 ggcgagacag caccgtcgat cgcatcgcgg cgctgccgat cggaccggcg cggcgagtgt
     3901 gggagatcgg ctgcggctcg gg
//
LOCUS       DASWNJ010000382         3394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_607436, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000382 DASWNJ010000000
VERSION     DASWNJ010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607436"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VGB85_06645"
     CDS             <1..194
                     /locus_tag="VGB85_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEY0133738.1"
                     /translation="RSSGFELTSLTALGLVSIELGYSNDRRCDARANCEVERASFVWR
                     DGLGRERTGEVVDVHLACQ"
     gene            298..1179
                     /locus_tag="VGB85_06650"
     CDS             298..1179
                     /locus_tag="VGB85_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133739.1"
                     /translation="MNTLTRIFPALVLLAALPACDKDEKDLAPGESESSGDTEGESSG
                     DSSGGSETGDAEVVCNEANLNKGRECEGGTEFCFLSSGNEYKWGSCIAEPACEPGVTL
                     DCEECEIDDDGDPYLVYTCAESTPLVLNFDGAPVEYGSAGKHFDLGTCAATDWPTART
                     PWLALDRDRSGHIDEGSELFGSATRMRAGSLADNGFTALSELDADRDGKITAADPKFS
                     ELVLWADGDADRRSSGLELQPLAALGLVSIELGYSSDRRCDARANCEVERASFVWRDG
                     LGREQTGEVIDVHLACQ"
     gene            1226..2380
                     /locus_tag="VGB85_06655"
     CDS             1226..2380
                     /locus_tag="VGB85_06655"
                     /inference="COORDINATES: protein motif:HMM:NF025580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HEY0133740.1"
                     /translation="MTPREHHRFFAKQLITTHTFQSPDKMFGELTGPKREAFLMFLWN
                     EAGNAVSSPIPHVAVEGGRLAKLEVVGALTRGDQQVVVLSMPPAIMPGEAAFIALTRG
                     SQGPVVFFLERTRDEAGTAMHPNDAVLAEVRADGMRISHGPASGIDLETFKSRVGAAL
                     GVSLDGIENSLPEITAAAFVGAGGARAAGKSMNPYAPPASGAPMPAAPQGGHVGGVLA
                     LLLLVRASVPLAVWVLSRVGLSGVIGPVYWLVAIAMTLLSLTIAVLMIVWLYQVHARR
                     RGQASFSPGMAVGAWFIPIANFVLIPLVVRSAWKATVGAGGGLLVLGWWLAWVAEIVL
                     STMRTTPELFSIAARALGNAIVPIMNYGVFVTVAAYGLLYYIVRRVNERL"
     gene            2383..>3394
                     /locus_tag="VGB85_06660"
     CDS             2383..>3394
                     /locus_tag="VGB85_06660"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEY0133741.1"
                     /translation="MARRPRGPDEQTLLGQFYTPPAVAELLVALTLDERADGPPEILD
                     PACGDGALLASAYESLVARGYSHTQVLAALSGFEISQTAADAASAALSARGAGAPKIL
                     AGDFFAREPEARFDCIIGNPPYLRSQNQDDLDPAYRERLFAAAARAGVRAHAKTDLFG
                     FFMMHASRFLKIGGRLGFVTPASWLTSEYAASLQGLLTGELRLSAIVSSSVESFFPEV
                     DIHTVLVLADRVDPAIEEPQAALRFVTLRRPLADLTAGGRHEQVQALAREVLGASAPR
                     EDGRLRVHVVPLAGERAALAAAPGVDWSRVTAFHMDEYIGLAADAPQRFGRYLRDHIF
                     AKV"
BASE COUNT          461 a         1150 c         1266 g          517 t
ORIGIN      
        1 gccggtcctc gggcttcgag cttacgtcgc tgaccgcgct ggggctcgtg agcatcgagc
       61 tcggctacag caacgaccgc cgctgcgacg cgcgcgccaa ctgcgaggtc gagcgcgcgt
      121 cgttcgtgtg gcgcgacggc ctcggccgcg agcgcaccgg cgaggtcgtc gacgtgcacc
      181 tcgcctgcca gtgagcgccg cgcgggcgcg ccccgtgagg tggcggcgct cgccgtcact
      241 tccagtcaat cctctcctgc tgccagactc gtcccttcgc cgatgaaagt aactgccatg
      301 aacaccctga cccgcatctt ccccgccctc gtcctgctcg ctgcgttgcc cgcctgtgac
      361 aaggacgaga aggacctcgc ccccggcgag tccgagagct cgggcgacac cgagggcgag
      421 agctcgggcg acagctcggg cggctcggag accggcgacg ccgaggtggt gtgcaacgag
      481 gcgaacctca acaagggtcg ggaatgcgag ggcgggacgg agttctgctt cctctcgagc
      541 ggcaacgagt acaagtgggg cagctgcatc gccgagcccg cgtgcgagcc gggcgtcacc
      601 ctcgactgcg aggagtgcga gatcgacgac gacggtgacc cgtacctcgt gtacacctgc
      661 gccgagagca cgccgctcgt cctcaacttc gacggcgcgc cggtcgagta cggcagcgcg
      721 ggcaagcact tcgacctcgg gacctgcgcg gcgaccgact ggccgacggc acgtacgccg
      781 tggctggcgc tcgaccgcga ccgctcgggc cacatcgacg agggcagcga gctgttcggc
      841 tcggcgacgc ggatgcgcgc cggcagcctg gccgacaacg gcttcacggc gctgtctgag
      901 ctcgacgccg accgcgacgg caagatcacc gcggccgacc cgaagttctc cgagctggtg
      961 ctgtgggccg acggcgacgc cgaccgccga tcctcgggcc tcgagctcca gccgctggcg
     1021 gccctgggcc tggtgagcat cgagctcggc tacagcagcg accgccgctg cgacgcgcgg
     1081 gccaactgcg aggtcgagcg ggcgtcgttc gtgtggcgcg acggcctcgg ccgcgagcag
     1141 accggcgaag ttatcgacgt acacctcgcc tgccagtgag cggccagcgc gccgggcccc
     1201 gggaaatgtg ataccagggg cccagatgac cccacgcgag caccaccgtt tcttcgccaa
     1261 gcagctgatc acgacgcaca cctttcagtc gccggacaag atgttcggcg agctgacggg
     1321 ccccaagcgc gaggcttttc tgatgttcct gtggaacgag gccggcaacg cggtctcctc
     1381 gccgatcccc cacgtggcgg tcgagggcgg gcggctggcg aagctcgagg tggtcggcgc
     1441 gctgacccgc ggcgaccagc aggtggtcgt gctgtcgatg ccgccggcga tcatgcccgg
     1501 cgaggcggcg ttcatcgccc tcaccagggg ctcgcagggg cccgtggtgt tcttcctcga
     1561 gcgcacgcgc gacgaggcag gcacggcgat gcacccgaac gacgcggtgc tcgccgaggt
     1621 gcgcgcggac ggcatgcgca tcagccacgg ccccgcgtcc ggcatcgacc tcgagacttt
     1681 taaaagtcgc gtcggcgcgg cgctcggggt gtcgctcgac ggcatcgaga acagcctgcc
     1741 ggagatcacg gcggcggcgt tcgtcggggc gggcggggcg agggccgcag gcaagagcat
     1801 gaacccgtac gcccctccgg cctccggcgc gccgatgccc gcggccccgc agggtgggca
     1861 cgtaggcggg gtcctggcgc tgctgctgct ggtgcgcgcg agcgtccccc tggcggtgtg
     1921 ggtgctgtcg cgcgtcgggc tcagcggcgt tatcgggccg gtgtactggt tggtggcgat
     1981 cgccatgacg ctgctgtcgc tgacgatcgc ggtgttgatg atcgtgtggc tgtatcaggt
     2041 gcacgcgcgg cgccgcggcc aggccagctt ctcgccgggc atggcggtcg gcgcgtggtt
     2101 catcccgatc gccaacttcg tgctcatccc gctggtcgtg cgcagcgcct ggaaggcgac
     2161 ggtcggggcc ggcggcgggc tgctggtgct cggctggtgg ctcgcgtggg tggcggagat
     2221 cgtgctgtcg acgatgcgca ccacccccga gctgttctcg atcgcggcgc gggcgctcgg
     2281 caacgccatc gtgccgatca tgaactatgg cgtgttcgtg acggtcgcgg cctacggcct
     2341 gctgtactac atcgtccgtc gcgtcaacga gcggctgtaa ccgtggcgag gcgcccgcgc
     2401 gggccggacg agcagacgct gctcggacaa ttttatacgc cgcccgcggt ggcggagctg
     2461 ctcgtcgcgc tgaccctcga cgagcgcgcc gacgggccgc cggagatcct cgaccccgcg
     2521 tgtggtgatg gggcgctgct cgccagcgcg tacgagtcgc tggtcgcgcg cgggtattca
     2581 catacgcagg tgctggcggc gctgtcgggc ttcgagatca gccagacggc ggcagacgcc
     2641 gcgagcgcgg ccctgtcggc gcgcggcgca ggggcgccga agatcctcgc gggtgacttc
     2701 ttcgcgcgcg agccggaggc gcgcttcgac tgcatcatcg gtaatccgcc gtatctgcgc
     2761 tcgcaaaacc aggacgacct cgaccctgca taccgcgagc ggctgttcgc ggcggcggcg
     2821 cgggcggggg tacgggcgca cgcgaagacc gacctgttcg gcttcttcat gatgcacgcc
     2881 tcgcggtttt tgaaaattgg cgggcggctc ggcttcgtca cgccggcgag ctggttgacc
     2941 tcggagtacg cggcctcgct gcaggggttg ctgactggcg agctgcggct gtcggcgatc
     3001 gtttcgtcga gcgtcgagtc gttcttcccc gaggtcgata tacacacggt gctggtattg
     3061 gcggacaggg tcgaccccgc gatcgaggag ccgcaggcgg cgctgcgctt cgtgacgctg
     3121 cggcgcccgc tcgcggacct gaccgccggt gggcggcacg agcaggtgca ggcgctggct
     3181 cgggaggtgc tgggggcgag cgcgccgcgg gaggacggac gcctgcgggt gcacgtcgtg
     3241 ccgctcgcgg gcgagcgggc ggcgctggcg gccgcgccgg gggtcgactg gtcgcgggtg
     3301 acggcgttcc acatggacga gtacatcggc ctggccgccg acgcgccgca gcggttcggg
     3361 cggtacctcc gcgaccacat cttcgcgaag gttc
//
LOCUS       DASWNJ010000383         2660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_218748, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000383 DASWNJ010000000
VERSION     DASWNJ010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218748"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /locus_tag="VGB85_06665"
     CDS             <1..1479
                     /locus_tag="VGB85_06665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0133742.1"
                     /translation="LRIGFVSVLSLFTACPDAGSVTGVVDEPDTTSTGAPDDTGSPTS
                     TGDAPTTHAFGDTEEPGDSTSSSTSGTSSTSGTSSETGNADTSSSSGGVVGVCGDGVV
                     EGDEQCDDGDVLPDDGCSPTCEVGPGAVLPSVELNLAEYEQLFCLAIVDKQHLGGRTH
                     GLTLGSVVEGAGPEGQRGAHVQQMPLAGGASSWSYLEHAGIHGRIPMEATTMSNGDVV
                     VAGLVWTEDLVADSGGYLWLARFTPTGQIVWSHDIEQVMTQAFDMAMTSDGDIVFAGH
                     TAGYSTGNLAHRFRGSDGALLWSYEEPQSVTHGGSYAGMTLDADDNVYIVGFRGMYVE
                     PPVPAWAHVRSLDASGAERWELEHKVADQNLLGWTITVTTDEQLIVSGVQYVDIEQPT
                     TTLFLGLDLDGALLWSQPWSPDVPQEIRFSAAAPDASGGLYVTGLVKEGLTHHRITAR
                     FDATGHPVWVNAGGEGWGRDIEVDPDGIVHVLTPAAVIAHLP"
     gene            complement(1549..1788)
                     /locus_tag="VGB85_06670"
     CDS             complement(1549..1788)
                     /locus_tag="VGB85_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133743.1"
                     /translation="MLVSVVTGPVVSMDPVVGVSLVVPLVGDSPVDVSLAVLAVLDDP
                     PVEVAVELLASPVLESPAPPEQPALATNTKEVTRA"
     gene            1850..2629
                     /locus_tag="VGB85_06675"
     CDS             1850..2629
                     /locus_tag="VGB85_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133744.1"
                     /translation="MGSAGCGQGMPAAGSLEVEVEGQQGKYIVSLPPGYDPATPYPLG
                     FAFHGRNRTGPDCQNGDCAGFQAVMQDQAILVYMTSLGGTGWEGDGERELNVEFFNKV
                     LDGLAASACVDESKVFVAGTSSGAHFVNVLGCRFGDRLLAIAPVAGYLPEKDNCVGEV
                     AALVIHGFKDPHVPLSNGEEAREFWRTRAGCSDQTVPPIAEVHASVEAAPETHACAVY
                     QGCAADQPVVWCEHSEGGYDDSTHGWPKFGGQQIWEFVQSL"
BASE COUNT          450 a          880 c          958 g          372 t
ORIGIN      
        1 ctcagaattg gcttcgtctc cgtcctatcc cttttcaccg cctgccccga cgccggcagc
       61 gtgaccggcg tcgtcgacga gccggacacg accagtacgg gcgccccgga cgacaccggc
      121 tcgccgacca gcacgggcga cgcaccgacc acccacgcgt tcggcgacac cgaggagccc
      181 ggtgacagca cgagcagcag cacgagcggc acgagcagca cgagcggcac gagcagcgag
      241 acgggcaatg ctgataccag cagctcgtcg ggcggggtcg tgggcgtgtg cggcgacggt
      301 gtggtcgagg gcgacgagca atgcgacgac ggcgacgtgc tgccggacga cggctgctcg
      361 ccgacctgcg aggtcgggcc gggagcggtg ctaccctcgg tcgagctgaa cctcgcggag
      421 tatgagcagc tgttctgcct ggcgatcgtc gacaaacagc acctcggcgg gaggacgcac
      481 ggcctgacgc tcggtagcgt ggtggaagga gccgggcccg aggggcagcg cggcgcacat
      541 gtgcaacaga tgccgctggc gggcggggcc agcagctggt catacctcga gcacgccggg
      601 atccatgggc gcatacccat ggaggcgacc acgatgagca acggcgacgt cgtcgtggcg
      661 ggcctggtct ggacagagga cttggtggcc gacagcggcg ggtatctgtg gctggcccgc
      721 ttcacgccga ccggacagat cgtgtggagc cacgacatcg agcaggtgat gacgcaggcc
      781 ttcgacatgg cgatgacctc cgacggcgac atcgtgttcg cgggccacac cgcgggttac
      841 agcaccggga acctcgccca tcgcttccgc gggtccgacg gcgcgctgct atggagttat
      901 gaggagccgc aatcggtgac ccacggcggc agctacgccg ggatgacgct cgacgccgac
      961 gacaacgtct atatcgtcgg gtttcgtggg atgtacgtcg agccgccggt gcccgcgtgg
     1021 gcccacgtga gatcgctcga tgcgagcggc gccgagcggt gggagctcga gcacaaggtc
     1081 gcggatcaga acttgctggg gtggacgatc accgtcacca ccgacgagca gctgatcgtc
     1141 agcggcgtgc aatatgtgga tatcgagcag ccgacgacga ccctcttcct cgggctcgac
     1201 ctcgacggcg cgctgctgtg gtcccagccg tggagcccgg atgtcccgca ggagatccgc
     1261 ttcagcgccg ccgcgcccga cgcgagcgga ggtctgtatg tcaccggcct cgtgaaggaa
     1321 ggcctcacgc accatcgcat caccgcgcgc ttcgacgcga ccggtcaccc ggtgtgggtc
     1381 aacgccgggg gcgagggctg ggggcgcgac atcgaggtcg acccggacgg gatcgtccac
     1441 gtgttgacgc ccgcggcggt catcgcccac ctgccgtgaa ctatctgcca tgaacaacgc
     1501 gcaggcgggc ccgtgatagt gttccgcgat gcttcgagct gtacgaattc acgcccttgt
     1561 gacctcttta gtgttcgttg cgagcgcggg ctgttcgggc ggcgcgggtg actcgagcac
     1621 cggcgaggcg agcagctcga ccgcgacctc gaccggcgga tcgtcgagca cggcgagcac
     1681 ggcgagcgag acatcgacgg gcgagtcacc gacgagcggc acgacgagcg agacgccgac
     1741 gaccggatcc atcgacacga ccgggcccgt caccaccgac acgagcacga cggaggcgac
     1801 ctcgaccggc gcggacagct cgagcgggac gaccggaccc gtcgctccga tgggcagcgc
     1861 cggctgcggg caaggaatgc cggccgccgg ctcgctcgag gtcgaggtcg aggggcagca
     1921 gggcaagtac atcgtctcgc tgccgccggg ttacgacccg gcgacgccgt acccgctcgg
     1981 cttcgcgttc cacggccgca accggaccgg gcccgactgc caaaacggcg actgcgcggg
     2041 cttccaggcg gtgatgcagg accaggcgat cctcgtgtac atgacctcgc tcggcggcac
     2101 cggctgggaa ggggacggcg agcgcgagct caacgtcgag ttttttaata aggtgctcga
     2161 cggtctggcc gcgagcgcct gcgtcgacga gtcgaaggtg ttcgtcgcgg gcaccagctc
     2221 gggcgcgcac ttcgtcaacg tgctcggctg ccgcttcggc gaccgcctgc tggcgatcgc
     2281 gccggtcgcc ggttacctgc ccgaaaagga caactgcgtc ggtgaggtcg ccgcgctggt
     2341 gatccatggt ttcaaggacc cgcacgtgcc gctgtcgaac ggcgaggagg cgcgcgagtt
     2401 ctggcgcacg cgcgccggct gcagcgatca aaccgtgcca ccgatcgccg aggtccacgc
     2461 gtccgtcgag gccgcgcccg agacccacgc ctgcgcggtc taccagggct gcgccgccga
     2521 ccagccggtc gtgtggtgtg aacacagcga gggcggctac gacgacagca cccacggctg
     2581 gccgaagttc ggcgggcaac agatctggga gttcgtgcag agcctgtgag cggcgatcag
     2641 ctcggcttgt acaggccgtc
//
LOCUS       DASWNJ010000384         2093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_78519, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000384 DASWNJ010000000
VERSION     DASWNJ010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78519"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VGB85_06680"
     CDS             <1..906
                     /locus_tag="VGB85_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133745.1"
                     /translation="TGEETTVRVVADTVAEARLVVAAARGEIRGRVLEVGGGPVTDAF
                     VNAVLEQPGIDVRNELRWGGSGPPTLTDADGNFTIDDLFVGPHALRAYRRGGGEAMVE
                     HIATGTTTTLTITPTAEVGGTLAGERPDEFAIYLVDERTMLQRSEEFYRTGGRWYFEA
                     LPAGTYDLFFESALGTTKQTITLAPDEQRTDLRVALAPPGRIRGQAVDAATQAPAAGL
                     KVVASPMAGRTRFSAPPPEVTTDADGRFEIAAVHAGPVLLGAHGGDPARPGMTGDLQT
                     VVESGRSVEVILPVRSFEPPSEPNP"
     gene            923..1696
                     /locus_tag="VGB85_06685"
     CDS             923..1696
                     /locus_tag="VGB85_06685"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEY0133746.1"
                     /translation="MTAVGSFEDALATLLRACGRACAPVGARRVIVAGDLEITVSTDR
                     EAITIDLSGWQHRFALDDEDEDAAALALDLVGAALFGAVRVAVDLYAGRPRRFTLEVL
                     QGDRWQTVSTQGSRPWNPFTAATRRTHRGDATRPQRDAATSVTALPWAPWAGACGFYG
                     ATSATAASELPVDGELDLHNFRPREVKPLVLAYIDACLARGIHELRIVHGKGVGNLRR
                     TVHAILARHPQVRGYRLGGHGAGSWGATIVDLSPGTGDQ"
     gene            complement(1704..>2093)
                     /locus_tag="VGB85_06690"
     CDS             complement(1704..>2093)
                     /locus_tag="VGB85_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133747.1"
                     /translation="ETFPFPWPLDASADRLTARQRECHDAIAGAAEALHVARQRWQYP
                     PELVVHDEPLAPGHPPRVTARDVEAGRELKRRTLSALYRVRPAWLVELHARLDRAVAA
                     AYGWPDDLDDDALLVRLLALNGERRGP"
BASE COUNT          287 a          771 c          760 g          275 t
ORIGIN      
        1 accggcgagg agacgaccgt gcgcgtcgtc gccgacaccg tcgcggaggc gcgcctcgtc
       61 gtcgccgcgg cgcgcggcga gatccgcggg cgtgtgctcg aggtcggcgg cggcccggtc
      121 accgatgcct tcgtgaacgc cgtgctcgag cagcccggca tcgacgtgcg caacgagctg
      181 cgctggggtg gcagcgggcc gccgacgctc accgacgcgg acggcaattt cacgatcgac
      241 gacctcttcg tcggtccgca cgcgctgcgg gcctaccggc gcggcggcgg cgaggcgatg
      301 gtggagcaca tcgcgaccgg cacgaccacg acgctgacga tcacgccgac ggccgaggtc
      361 ggcggcacgc tcgcgggcga gcggcccgac gagtttgcga tctacctcgt cgacgagcgg
      421 acgatgttgc agcgcagcga ggagttctac cgcaccgggg gtcgctggta cttcgaggcg
      481 ctgccggccg gcacctacga tctgttcttc gagtcggcgc tcgggaccac gaagcagacg
      541 atcacgctgg cgcccgacga gcagcgcacg gacctgcgcg tcgccctcgc gcccccgggg
      601 cggatccgcg gccaggcggt cgacgcggcc acgcaggcgc ccgcggcggg cctgaaggtc
      661 gtcgcctcgc cgatggcggg acgaactcgc ttcagcgcgc ccccgccgga ggtcacgacc
      721 gatgccgacg gccgcttcga gatcgccgcc gtgcacgccg gcccggtcct gctcggcgcg
      781 cacggcggtg acccggcgcg ccccggcatg accggcgacc tccagaccgt cgtcgagtcc
      841 gggcgcagcg tcgaggtcat actgccggtg cgatcgttcg agccgccctc ggagccgaac
      901 ccgtgacatc ctcgcatccg tcatgaccgc cgtcggatcg ttcgaggacg cgctcgcaac
      961 cctgctgcgg gcctgtggac gggcctgcgc gccggtcggg gcgcgccgtg tcatcgtcgc
     1021 cggcgatctc gagatcacgg tgtccaccga ccgtgaagcg atcacgatcg acctctcggg
     1081 ctggcagcat cgcttcgcgc tcgacgacga ggatgaggac gccgcggccc tcgccctcga
     1141 tctcgtcggc gcggcgctgt tcggcgcggt ccgggtcgcg gtcgacctgt atgccggacg
     1201 gccgcggcgc ttcaccctcg aggtcctgca aggcgaccgc tggcagacgg tgagcacgca
     1261 gggctcgcgg ccatggaacc ccttcactgc cgccacgcgt cgcacccacc gcggcgacgc
     1321 gacgcgtccg cagcgagatg ccgcgacgtc agtgacggcc ctgccgtggg cgccgtgggc
     1381 aggcgcctgc gggttttatg gcgccacgtc cgcgaccgcg gccagcgagt tgccggtcga
     1441 cggcgagctc gacctgcaca acttccgccc gcgcgaggtc aagccgctcg tgctggcata
     1501 catcgacgcc tgcctcgcgc gtgggatcca cgagctgcgc atcgtccacg gcaagggcgt
     1561 cggcaacctg cgccgcaccg tgcacgcgat cctcgccaga cacccgcagg tccgcggcta
     1621 tcgcctcggc ggacacggcg cgggcagctg gggcgcgacg atcgtcgacc tgtctcccgg
     1681 gacaggagat caataaagcg ggatcacggg ccgcgccgct cgccgttgag ggccagcagc
     1741 cggacgagca gcgcgtcgtc gtcgaggtcg tccggccagc cgtacgcggc cgcgacggcg
     1801 cggtcgagcc gcgcgtgaag ctcgaccagc cacgccggcc gcacgcggta cagcgcgctc
     1861 agcgtgcgtc gtttgagctc gcgcccggcc tcgacatcac gcgcggtcac gcgtggcgga
     1921 tgaccggggg cgagcggttc atcatgcacg accagctcgg gcgggtattg ccagcgctgt
     1981 cgcgcgacgt gcaacgcctc cgcggcgcct gcgatcgcat catgacattc gcgctgccgc
     2041 gcggtgagcc ggtccgcgga cgcgtcgagc ggccacggga acgggaacgt ctc
//
LOCUS       DASWNJ010000385         1906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_183670, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000385 DASWNJ010000000
VERSION     DASWNJ010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183670"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VGB85_06695"
     CDS             <1..476
                     /locus_tag="VGB85_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133748.1"
                     /translation="RTQPWPGWATEYLELPASSSAESLVRALPPARRKPFARTSEARY
                     RERLADWTGGHEGLSWLAARLRHHGWWLVHNLVAHPLLALVPLRPGVALHDWTSRRLN
                     LEAAIEPSPAPRIDRRLWWLVHNFVSHPAIGLFPCARTFRWHDVTARRMAVHGWA"
     gene            complement(593..1792)
                     /locus_tag="VGB85_06700"
     CDS             complement(593..1792)
                     /locus_tag="VGB85_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEY0133749.1"
                     /translation="MTLRPLFALSAAILLAACSLETKLGELTESDETSDPSAGSDGDP
                     TTTGADTAPPINSGSDDSGSEATGDSDTDGGSCTPVTVTRAPLSPNVVLVLDKSGSMV
                     ADPSGLWDHDADPNTPPVTRWSSLHQVVEAITNEFDDTINFGAQLFPGVDAKADYSAL
                     ACTINEDIDIAVSAMNKDVIFAGIPAADDTSMRGGTPMAAGVTTALEHLKTLDAQIPR
                     AILLVTDGAANCIDDADPEVMFEQYDESIHSIVGDAFALDGIPTYVVGVGIQDLVSSD
                     AKDGSPDSVNTFTRLNELATDGGKPKNDPGEKFFNTVNQSELAAALTEIAVDTLTCII
                     PLDPPLNAPDEITVEVDGMQIPHISDCADQSGYAHPDGPFDAIHLCGAACGGLKLAGE
                     AAITLCM"
BASE COUNT          254 a          603 c          718 g          331 t
ORIGIN      
        1 cgcggacgca gccatggccg ggatgggcga ccgaatatct cgagttgccc gcgagcagca
       61 gcgccgagag tctcgtccgc gcgctcccgc cggcgcgtcg caagccgttc gcgcgcactt
      121 ccgaggcccg ctaccgcgag cgactggcgg actggaccgg cggacacgag gggctgtcgt
      181 ggctcgccgc ccggctgcgc catcacggct ggtggctcgt gcacaacctc gtcgcccacc
      241 cgctgctcgc gctggtcccg ctgcggcctg gcgtcgcgct gcacgactgg accagccgcc
      301 gcctcaacct cgaagcggcc atcgagccct cgcccgcgcc gcggatcgat cgacgtttgt
      361 ggtggctcgt gcacaatttt gtcagccacc ctgcgatcgg cctgttccca tgcgcccgca
      421 cgttccgctg gcatgacgtc acggcccggc gcatggccgt ccacggctgg gcctgaccgg
      481 gcaaaagcaa gtcgcggacg cgccgggaca ggcgcatccg cgtagtatcg attaatgtaa
      541 gtcgcgcagg gacaggcgcg tttgctggtc acggagccgg atcggcccgc ttttacatgc
      601 acagcgtgat cgcggcctcg ccggcgagct tgagcccgcc gcaggccgcc ccgcacaggt
      661 ggatcgcgtc gaaggggccg tcggggtggg cgtagccgct ttggtcggcg cagtcgctga
      721 tgtgggggat ctgcatgccg tcgacctcga cggtgatctc gtcgggggcg ttgagcggcg
      781 ggtcgagggg gatgatgcag gtcagggtgt cgaccgcgat ctcggtgagg gccgcggcga
      841 gctcgctctg gttgaccgtg ttgaagaact tctcgccggg gtcgttcttc ggcttgccgc
      901 cgtcggtggc gagctcgttg aggcgggtga aggtgttgac gctgtcgggg ctgccgtcct
      961 tggcgtcgga gctgacgaga tcctggatgc cgacgccgac gacgtaggtc gggatgccgt
     1021 cgagggcgaa ggcgtcaccg acgatgctgt ggatgctttc gtcgtactgc tcgaacatga
     1081 cctcggggtc ggcgtcgtcg atgcagttgg cggcgccgtc ggtgacgagg aggatcgcgc
     1141 gcgggatctg ggcgtcgaga gtcttgaggt gctcgagcgc cgtcgtgacg ccggcggcca
     1201 tcggcgtgcc gccgcgcatc gaggtgtcgt ccgcggccgg gatgccggcg aagatcacgt
     1261 ccttgttcat cgcagagacg gcgatatcga tgtcctcgtt gatcgtgcag gcgagcgcgc
     1321 tgtagtcggc cttggcgtcg acgccgggga acagctgggc gccgaagttg atcgtgtcgt
     1381 cgaactcgtt cgtgatggcc tcgacgacct gatgcaggct cgaccagcgg gtcacgggcg
     1441 gggtgttcgg gtcggcgtcg tggtcccaca ggccgctcgg gtcggcgacc atcgagcccg
     1501 acttgtcgag cacgagcacc acgttcgggc tcagcggcgc gcgcgtcacg gtgacgggcg
     1561 tacaggagcc gccgtccgtg tcggagtcgc cggtcgcctc gctgcccgag tcgtcgctgc
     1621 cgctgttgat cggaggggcc gtgtcggcgc cggtggtggt cggatcgccg tcggatccgg
     1681 cggaggggtc actggtctcg tcgctctcgg tcagctcgcc gagcttggtc tcgagcgagc
     1741 aggcggcgag caggatcgcg gccgagagcg cgaacagggg gcgaagggtc actgtcctta
     1801 tgggcatgtg catcgaaggt cctcacatcc ccgtctccgc gatccggagg atccgtcacc
     1861 gggtgcaacg ataattcgcg aagcgctgcg cgacggccga ggcggg
//
LOCUS       DASWNJ010000386         1646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_254986, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000386 DASWNJ010000000
VERSION     DASWNJ010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254986"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VGB85_06705"
     CDS             <1..215
                     /locus_tag="VGB85_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133750.1"
                     /translation="GAKEWLSKHFPRGSSYKPTLDQLPMTRMIDFDRLRSCDLPCFGT
                     LERALHFLAENRGRAGGVYPLDPTSR"
     gene            complement(243..>1646)
                     /locus_tag="VGB85_06710"
     CDS             complement(243..>1646)
                     /locus_tag="VGB85_06710"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY0133751.1"
                     /translation="AASVVGVPEPPAPVEPSPAEAVTLAEPPAPEVPAPSGIRVSTRT
                     TIVYAAPRYGSEMRGRIDRGAPFAIYGLVEGSGCDGEGWARAGHREGDGFVCLKSASA
                     TEAAPQVLPIVPEGQVVPFIYAKPKADRKGKLLAEVPRWRTRHALTSGREPEDYLEAN
                     RQYAFVDLRPIPGHGKIYEDADERVVLARDMRPEQPSDFHGRVLAERPIAEGRMAAWV
                     SSREAEMRAEPKASAPVAAVPAFHAELDALAEIQQVGRRRWVVVPDALGPGVPGYIET
                     TMVRMYEPGPALDDIGDEETWVDVDVGQQTLAVMQGQRPIFVTLISSGTGSKPNTATP
                     RGVYRIRNKVAFGAMRNRPEDAEESPYHVEAVPWVQYFYRRFALHGAYWHDGFGHRKS
                     HGCVNLAPRDARYVYGLTTPNVPPGWMSSYEHAGEIGSVVRVRKGQELGEDRRQINDV
                     EQDAAAAADTVLADGAP"
BASE COUNT          210 a          583 c          600 g          253 t
ORIGIN      
        1 gtggcgccaa ggagtggctg tcgaagcact tccctcgcgg ctctagctat aagcccacgc
       61 tggatcagct tccgatgacc cgcatgatcg acttcgatcg cctacgaagc tgcgacttgc
      121 cgtgcttcgg aaccctcgaa cgggcgctcc acttcctcgc ggagaaccgc gggcgcgccg
      181 gcggcgtcta cccgctggac ccaaccagca ggtgagcgcc ccgggccgtc gagggtccgc
      241 gatcacggcg cgccgtcggc gagcaccgta tctgccgcag ccgcggcgtc ctgctcgacg
      301 tcattgatct ggcgccggtc ctcgccgagc tcctggccct tgcgcacgcg cacgacgctg
      361 ccgatctcgc cggcgtgttc gtagctcgac atccagcccg ggggcacgtt cggggtcgtc
      421 aggccgtaga cgtagcgggc gtccctcggg gccaggttga cgcagccgtg gctcttgcgg
      481 tggccgaagc cgtcgtgcca gtaggcgccg tgcagggcga agcgacgata aaaatactgc
      541 acccacggga cggcctcgac gtggtacggc gactcctcgg cgtcctcggg gcggttgcgc
      601 atcgcgccga aggcgacctt gttgcggatg cgatagacgc ctctcggcgt cgcggtgttg
      661 ggcttgctgc cggtgccgct cgagattagc gtcacgaaga tcgggcgctg gccctgcatg
      721 acggcgaggg tctgttgccc gacgtcgacg tcgacccagg tctcctcgtc gccgatgtcg
      781 tcgagcgccg ggccgggctc gtacatgcgg accatcgtcg tctcgatgta gccgggcacg
      841 ccggggccga gggcgtcggg cacgaccacc cagcggcggc gcccgacctg ctggatctcc
      901 gcgagcgcgt cgagctccgc gtggaaggcg ggcacggcgg cgaccggcgc gctggctttg
      961 ggctcggcgc gcatctcggc ctcacgcgag ctgacccacg cggccatgcg gccctccgcg
     1021 atcggtcgct cggcgagcac gcgcccgtgg aagtcgctcg gctgctcggg ccgcatgtcg
     1081 cgggcgagca cgacgcgctc gtcggcgtcc tcgtagatct tgccgtgccc ggggatcggg
     1141 cgcaggtcga cgaaggcgta ttggcggttg gcctcgaggt agtcctccgg ctcgcggccg
     1201 ctggtcagcg cgtggcgggt gcgccagcgc gggacctcgg cgagcagctt gcccttccgg
     1261 tcggccttgg gcttggcgta gatgaacggc acgacctggc cctcgggcac gatcggtagt
     1321 acctgcggcg cggcctcggt cgcgctcgcg gacttgagac acacgaagcc gtcgccctcg
     1381 cggtgaccgg cgcgcgccca gccttcaccg tcgcaacctg acccttcgac caggccgtag
     1441 atcgcaaagg gcgccccgcg gtcgatgcgt ccgcgcatct cgctgccgta acgcggcgcg
     1501 gcgtagacga tcgtcgtgcg cgtcgagacg cggatgccgg acggcgccgg gacctcgggc
     1561 gcgggcggct cggcgagcgt caccgcctcg gcgggcgacg gctcgaccgg cgccggcggc
     1621 tcggggaccc ccacgaccga tgcggc
//
LOCUS       DASWNJ010000387         2133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_537878, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000387 DASWNJ010000000
VERSION     DASWNJ010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537878"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..>2133
                     /locus_tag="VGB85_06715"
     CDS             150..>2133
                     /locus_tag="VGB85_06715"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0133752.1"
                     /translation="MTESAQDPAQIGPFVVLRKLGEGAMGVVYAGFDVQLDRKVAIKL
                     VHRHLLDRPEVRLRMTREAQAMARLSSPHVVHVYQVGEHDDGIYMAMEYIDGLTLGAW
                     LRARPRPWQLVLRTVCDAGRGLAAAHHAGLTHRDFKPDNVLVDADGRARVLDFGLAQT
                     DAAGQHEQAMAQPETVPTEEEEVHALGDDIHWSVRLTERGKALGTPAYMSPEQHFGGP
                     VGPVSDQFSFSITLYEALYGYRPFYGDTWNAIKQQVIRGVVPPPPVETRVPKRVYRAL
                     VRGLAIAPDARWPSLDAMLDALEHDPWRVRMRVAGVAGLIGVASAASYAVAVTQISPV
                     TSCDATAELAGVWDHERRTAVSRAFTATRAPFAAEVQERTLARLDAYAASWTAEHTAA
                     CEANAAGRQTNHVMDLRSACLGRRKSQVAALVDIFTEADRAVVERAVQAVAALPSVKA
                     CADVDGLLTAVPPPSDPTTAARVEDLRERLARATSLEATGQYEQGLQLAAQVRAEARI
                     LGYAPLDAEAALVEGKLLMVATKAETADAALAHALRVAITHDLKLVAAEAAAVRLFVI
                     SDGLGRPAEALVTRPIAEALVERASGDGQLSALLSNNLGAAYERAGDVPAARGHYERS
                     LELLQRASAGDPLSAVIHHNLGGMYLDEGQLGQARVH"
BASE COUNT          296 a          785 c          771 g          281 t
ORIGIN      
        1 cggtgcgatc tgcctcaacg ccgaaaacac gcgcctgccc gatcaggatg tcgcttgcga
       61 gctcactccg tgcggcgccg acttcgcctc gggcggcctg atccagagcg gcaaggtgct
      121 cggcttgctg caataatgac ggcggggtgg tgaccgaaag cgcccaggac ccggcccaga
      181 tcggcccctt cgtcgtgctg cgcaagctcg gtgagggggc catgggcgtg gtctacgccg
      241 gcttcgacgt ccagctcgat cgtaaggtcg cgatcaagct cgtgcatcgt cacctgctcg
      301 accgacccga ggtccgcctg cggatgaccc gcgaggccca ggcgatggcc cgcctgtcga
      361 gccctcacgt cgtccacgtg taccaggtcg gcgagcacga cgacggcatc tacatggcga
      421 tggagtacat cgacgggctg acgctcggcg cctggctgcg cgcgcgcccg cggccgtggc
      481 agctggtgct gcgcaccgtc tgtgacgccg gtcgtggcct cgccgcggcg caccacgccg
      541 gcttgaccca ccgcgacttc aagcccgaca acgtgctcgt cgacgccgac ggtcgcgcgc
      601 gcgtcctcga cttcggcctc gcgcagaccg acgccgccgg ccagcacgag caggcgatgg
      661 cgcagccgga gacggtgccg accgaggagg aggaggtaca cgcgctcggc gacgacatcc
      721 actggtcggt gcggctgacg gagcgaggca aggcgctggg gaccccggcg tacatgtctc
      781 cggagcagca cttcggcggc ccggtcggcc cggtctcgga tcagttcagc ttcagcatca
      841 cgctgtacga ggcgctgtat ggataccggc cgttttacgg cgacacctgg aacgcgatca
      901 agcaacaggt gatccgcggc gtggtcccgc cgccgccggt ggagacgcgc gtgcccaagc
      961 gggtgtatcg cgcgctggtc cgcggcctgg cgatcgcccc cgacgcgcgc tggccgagcc
     1021 tcgacgcgat gctcgacgcg ctcgagcacg acccgtggcg cgtgcgcatg cgcgtcgccg
     1081 gcgtcgccgg tctgatcggc gtcgcgtcgg ccgccagcta cgccgtcgcc gtcacccaga
     1141 tctcccccgt caccagctgc gacgcgaccg cggagctcgc cggcgtgtgg gatcacgagc
     1201 gcaggacggc cgtctcgcgc gcgttcactg cgacccgcgc cccgttcgcc gccgaggtcc
     1261 aggagcgcac gctcgcgcgc ctcgacgcct acgcggcgtc ctggaccgcg gagcacacgg
     1321 cggcctgcga ggccaacgcc gccggccggc agaccaacca tgtcatggac ctgcgcagcg
     1381 cgtgcctcgg gcgccgcaag tcgcaggtcg ccgcgctggt cgacatcttc accgaggccg
     1441 accgcgcggt cgtcgagcgc gcggtgcagg ccgtcgcggc gctgccgagc gtcaaggcct
     1501 gcgcggacgt cgatggtctg ctgacggccg tgccgccgcc gagcgacccg acgacggcgg
     1561 cccgggtcga ggacctgcgc gagcggctgg cgcgggcgac ctcgctcgag gcgaccgggc
     1621 agtacgagca gggcctgcag ctggccgcgc aggtgcgtgc ggaggcgagg atcctcggct
     1681 acgcgccgct cgacgccgag gccgcgctcg tcgagggcaa gctgctgatg gtcgcgacga
     1741 aggccgagac cgcggacgcg gcgctggccc acgcgctgcg cgtggcgatc acccacgacc
     1801 tgaagttggt cgcggccgag gccgcggcgg tgcgcctgtt cgtgatcagc gacgggctcg
     1861 ggcgtccggc cgaggcgctc gtcacccgcc cgatcgccga ggcgctggtc gagcgcgcca
     1921 gcggggacgg ccagctctcc gcgctgctga gcaacaacct cggcgccgcc tacgagcgcg
     1981 ccggcgacgt ccccgcggcc cgcggacact acgagcgctc gctcgagctg ctgcaacgcg
     2041 cgagcgccgg tgacccgctg agcgccgtca ttcatcacaa cctcggcggc atgtacctcg
     2101 acgagggcca gctcgggcag gcccgcgtgc att
//
LOCUS       DASWNJ010000388         1911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_642915, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000388 DASWNJ010000000
VERSION     DASWNJ010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642915"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1911)
                     /locus_tag="VGB85_06720"
     CDS             complement(<1..>1911)
                     /locus_tag="VGB85_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEY0133753.1"
                     /translation="VADDNADMRNYLARILSAYWTVEAVCDGAQALAAVRERRPDLVL
                     SDVMMPEIVGLQLVAALRADPAVATIPVLLLSARAGEAATIEGLESGADAYLIKPFAA
                     NELVARVRAQLASARARQEAARVQRDHVETTERLLAQARDATRARDEMLAVVSHDLRN
                     PLAVIGTSVDLLARSLGDDERAPRLRRSVDTIRRAVGGMSRLIADLLDSASIDAGSLS
                     LDPAPHTVDALLRELWEMFESQAAGKGVAFSVEVAAGVPALVCDRQRILQALGNLVAN
                     ALKFTAPPGSVLVRATAERDGGAVRFRVIDDGPGIAEDLRLHLFDRHWLAQRHSSDGH
                     GLGLSIAKGIVEKHGGQIDVDSTPGRGTTFSLALPVTPGALSVATATPATPALAETRP
                     GRPEDTFLRGGGEMGARTRAFDWSTTSLGAAEAWPQSLRTTVSTMLRSPYPITMFWGP
                     ELVMLNNDAFAPIHGAKHPAVLGMPAPTALAEAWGQLGPLVEGVLASGESLFIEDAAV
                     VFERGVGGVKEEAYFTWCYVPTADETGGIAGLFAISSETTRQVVGGRRLTVLRELSIR
                     TALDKTVDAVFRSVEDVLAQAGADLPFALLYTVEGDAARLVACVGVERGTAAASASPA
                     LDEPWPLGEVAHA"
BASE COUNT          258 a          721 c          688 g          244 t
ORIGIN      
        1 cgcgtgggcg acctcgccga gcggccacgg ctcgtcgagg gccggcgacg cgctggcggc
       61 cgcggtgccc cgctcgacgc cgacgcacgc gacgaggcgc gcggcgtccc cctcgaccgt
      121 gtagagcagc gcgaacggca ggtcggcgcc ggcctgcgcg agcacgtcct cgaccgaccg
      181 gaacacggcg tcgacggtct tgtcgagggc ggtgcggatc gacagctcgc gcaggaccgt
      241 caggcgccgg ccaccgacca cctgccgcgt ggtctcgctc gagatggcga acagaccggc
      301 gatgccgccc gtctcgtcgg cggtcgggac gtaacaccac gtgaagtagg cctcttcctt
      361 cacgccgccg acgccgcgct cgaacacgac ggccgcgtcc tcgatgaaca gcgactcgcc
      421 cgaggcgagc acgccctcga cgagcgggcc gagctgcccc cacgcctccg ccaacgccgt
      481 cggcgccggc ataccgagca ccgcggggtg cttcgccccg tggatcggcg cgaacgcgtc
      541 gttgttgagc atcaccagct cggggcccca gaacatcgtg atcgggtacg gcgagcgcag
      601 catcgtgctg accgtggtgc gcagcgactg cggccaggcc tccgcggcgc cgagcgaggt
      661 cgtcgaccag tcgaacgcgc gcgtgcgggc ccccatctcg ccgccgccgc gcaggaacgt
      721 gtcctcgggt cggcccggcc gggtctccgc gagcgcgggc gtggccggcg tcgcggtcgc
      781 tacggacagc gcccccggtg tgacgggcag cgccagcgag aacgtggtcc cgcggcccgg
      841 cgtgctgtcg acatcgatct gcccgccatg cttctcgacg atgccctttg cgatcgagag
      901 gccgaggccg tggccgtcgc tgctgtgccg ctgggcgagc cagtgacgat cgaagaggtg
      961 cagtcggagg tcctccgcga tccccggccc gtcgtcgatc acgcggaagc gaacggcgcc
     1021 gccgtcccgc tcggccgtcg cccgcacgag caccgaaccc ggcggcgcgg tgaacttcag
     1081 cgcgttggcg acgaggttgc cgagcgcctg caatatgcgc tgcctatcgc ataccagtgc
     1141 gggcacgccg gccgcgacct cgaccgagaa ggcgacgccc ttgccggccg cctgcgactc
     1201 gaacatctcc cacagctcgc gcagcagcgc gtcgaccgtg tgcggcgcgg gatcgaggga
     1261 cagcgagccg gcgtcgatgc tggcgctgtc gagcagatcg gcgatcagcc ggctcatccc
     1321 gccgaccgcg cgacggatcg tgtcgacgga ccgccgcagt cgcggcgcgc gctcgtcgtc
     1381 cccgagcgaa cgcgcgagca gatcgaccga ggtgccgatc accgccagcg ggttgcgcag
     1441 gtcgtggctg acgaccgcga gcatctcgtc gcgcgcccgc gtcgcgtcgc gggcctgcgc
     1501 gagcagccgc tcggtcgtct cgacgtggtc tcgctggacg cgggcggcct cctgtcgcgc
     1561 gcgtgcgctc gccagctggg cccggacgcg ggcgaccagc tcgttggccg cgaacggctt
     1621 gatcaggtac gcgtccgcgc cgctctcgag cccctcgatc gtcgccgcct cgcccgcgcg
     1681 cgccgacagc agcagcacgg ggatcgtcgc cacggccggg tcggcgcgca gcgcagcgac
     1741 gagctgcagg ccgacgatct ccggcatcat cacgtcgctg agcacgaggt cgggccgtcg
     1801 ctcgcggacc gccgccagcg cctgcgcgcc gtcgcatacc gcctcgaccg tccagtacgc
     1861 cgacaggatc cgcgcgaggt aattgcgcat gtcggcgttg tcgtcggcga c
//
LOCUS       DASWNJ010000389         3075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_394475, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000389 DASWNJ010000000
VERSION     DASWNJ010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394475"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..764
                     /locus_tag="VGB85_06725"
     CDS             93..764
                     /locus_tag="VGB85_06725"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY0133754.1"
                     /translation="MTEISKLTDLELLLGWRKGDKAMGAQLFERHKTAVTNLFRRNVR
                     NKESIPDLVQQTFLACIDAKNDPQVSGSVRSYILGIAFHTMTRFFRKELKAPALGLEE
                     ESGTSLASVEPDPEYLLTLSDEQRLLMKALRRLTIEFQAVIELNYWEGVSCDDIAEML
                     NIPQGTARRRLQRGREALEKKLEELSDSPELLRSTTMSLHTWQRSIHEWIAARPDAPG
                     RCPPN"
     gene            799..>3075
                     /locus_tag="VGB85_06730"
     CDS             799..>3075
                     /locus_tag="VGB85_06730"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0133755.1"
                     /translation="MSASNEETLHPIDRWARAVCETAAPQDRAGRSLEDAARLQLFGP
                     AMNAITQQDPEGRHLEDQAFAGLFGGPAPAPPAPQRIGKYVLGGPLGSGGMGDVFLAL
                     HTELHRKVAIKLLRVHKQRDPVERSRLLREAQALAQLDHNNVVQVYDSGLHEDRVYLV
                     MKYVEGCTLRAWQDAAHRSWREVVQHYVAAGRGLAAIHAAGLVHRDFKPQNVLVSALD
                     KDRPTDRASVAERVKITDFGLAVTPGERTAGTTVADAQGQTPGLLAMQLTASASVLGT
                     PLYVSPEQLGAQPLDARSDQFSFCVALYEALYGEHPYAEPDTASDSRAPTDNQTRAAV
                     GTRPSATPSMASLVTGLVQGPLRRPKKIVIPGRIYRAIERGLSREPAGRYPTMAELLA
                     ALERDPVRKYGTLVGMAGLGALVVGAAVAPQLLSKCNDIAADFKDAWTPRRAADVERA
                     FLATDSPHAALAAARVKQTLDAYSEQWLQLRAENCDTGRDRGTQTLEVTGRRDTCLDH
                     ARDTLAATVEQLTHADATTVETVQDALALLPALEQCDAKFQRQQTCLDEADDPQLRHR
                     LDEARALEIAGKYVDAGEVATTVLADAGAHPRLMAESQLLRGRVLAEQDRSIEADAAF
                     AAAYGLSEGAGCDTLAFEAATRRTKLIALNHDLPAETGLAWSEVARSKLGALPPSPRL
                     EADLHSDLGLLLQQREDLGGSREEHLARAREEHGRALELRKQLAGDRETPELAFSYLN
                     LGALEVSQGRHELAVEDLQ"
BASE COUNT          511 a         1184 c          994 g          386 t
ORIGIN      
        1 gatcggcggg gccgacggca ccatgtagga cgcgcaaagg tccccgacca cacgaggaag
       61 cacccgacga agaaccgggg gagatcactg tgatgaccga aatctcaaaa ttgaccgacc
      121 tcgagctcct cctcggctgg cgtaaaggcg acaaggcgat gggcgcccag ctgttcgagc
      181 gccacaagac cgccgtcacc aacctgtttc gtcgcaacgt ccgcaacaag gagagcatcc
      241 cggacctggt ccagcaaact tttttggcgt gcatcgacgc caagaacgac ccgcaggtct
      301 cgggcagcgt gcgcagctac atcctcggca tcgcctttca cacgatgacc cgcttctttc
      361 gcaaggagct caaggcgccg gcgctcgggc tcgaggagga gtcgggcacc tcgctcgcct
      421 cggtcgagcc cgaccccgaa tatctgctga ccctcagcga cgagcagcgg ctgctgatga
      481 aggctctgcg ccgcctgacg atcgagtttc aggccgtcat cgagctcaat tactgggagg
      541 gcgtctcgtg cgacgacatc gccgagatgc tcaacatccc gcaggggacc gcgcgccgcc
      601 ggctgcagcg cgggcgcgag gcgctcgaaa agaagctcga ggagctgagc gactcgcccg
      661 agctgctgcg ctcgacgacg atgtcgctgc atacctggca gcgcagcatc cacgagtgga
      721 tcgccgccag gcccgacgcg ccgggcagat gtccgccgaa ttgatcgacg ccgtcccggg
      781 gcggcacatc cctcgcgtat gtctgcgagc aacgaagaga ccttgcatcc catcgaccgc
      841 tgggcccgcg ccgtctgcga gaccgccgct ccgcaagacc gcgccggccg cagcctcgag
      901 gacgccgccc gcctgcagct gttcggcccc gccatgaacg ccatcaccca gcaagacccc
      961 gaaggccgcc acctcgagga ccaggccttc gccggcctgt tcggcggccc cgcccccgcc
     1021 ccgcccgcgc cccagcggat cggcaagtac gtcctcggcg gcccgctcgg cagcggcggc
     1081 atgggcgacg tgttcctcgc gctccacacc gagctgcacc gcaaggtcgc gatcaagctg
     1141 ctgcgcgtac ataaacagcg cgacccggtc gagcgcagcc gcctgctgcg cgaggcccag
     1201 gcgctcgccc agctcgacca caacaacgtc gtgcaagtct acgactcggg cctgcacgag
     1261 gaccgcgtct acctggtcat gaagtacgtc gagggctgca cgctgcgcgc ctggcaggac
     1321 gccgcgcacc gcagctggcg cgaggtcgtg cagcactacg tcgctgccgg ccgcgggctc
     1381 gctgcgatcc acgcggccgg cctggtccac cgcgacttca agccgcaaaa cgtgctcgtc
     1441 agcgccctcg acaaggacag gccgaccgac agggccagcg tcgccgagcg ggtcaagatc
     1501 accgacttcg gcctcgcggt cacgcccggc gagcgcacgg ccggcaccac cgtcgccgac
     1561 gcacagggcc agacgcccgg cctgctcgcc atgcagctga ccgcgagcgc ctcggtgctc
     1621 ggcacgccgc tctacgtctc accggagcag ctcggggccc agcccctcga cgcgcgcagt
     1681 gatcagttca gcttctgcgt cgcgctctac gaggcgctgt acggcgagca cccttacgcc
     1741 gagcccgaca ccgccagcga cagccgggcg cccaccgaca accagacgcg cgccgccgtc
     1801 ggcacgcgcc cctcggcgac gccgagcatg gcgtcgctgg tcaccggcct cgtccaggga
     1861 cccctgcgca ggccgaagaa gatcgtgatc cccgggcgta tctatcgggc gatcgagcgc
     1921 ggcctgtcgc gtgagcccgc cgggcgctac ccgacgatgg ccgagctgct cgccgcgctc
     1981 gagcgcgacc cggtgcgcaa gtatggaact ttggtcggca tggccggcct gggcgcgctg
     2041 gtcgtcgggg ccgccgtggc cccgcagctg ttgagcaagt gcaacgacat cgcggcggac
     2101 ttcaaggacg catggacgcc ccggcgcgcc gctgacgtcg agcgtgcgtt cctcgccacg
     2161 gactcgcccc acgcggccct cgccgccgcc cgcgtcaagc agacgctcga cgcctatagc
     2221 gagcaatggt tgcagctgcg cgccgagaac tgcgacaccg gccgcgaccg cggcacccag
     2281 acgctcgagg tgaccggccg ccgcgacacc tgcctcgacc acgcccgcga caccctcgcc
     2341 gcgaccgtcg agcagctcac ccatgcggac gccaccaccg tcgagaccgt gcaggacgcg
     2401 ctcgccctgc tgcccgcgct cgagcagtgc gacgccaagt tccagcgcca gcagacctgc
     2461 ctcgacgagg ccgacgaccc gcagctgcgc caccgcctcg acgaggcccg cgccctcgag
     2521 atcgccggca agtacgtcga tgccggtgag gtcgccacga ccgtgctcgc cgatgccggc
     2581 gcccatccgc gcctgatggc cgagtcgcag ctgttacgcg gccgcgtgct cgccgagcag
     2641 gaccgctcca tcgaggccga cgccgccttc gcggccgcct acggcctgtc cgaaggggca
     2701 ggttgcgaca ccctcgcctt cgaggccgcg acccggcgca ccaagctgat cgcgctcaac
     2761 cacgacctgc ccgcggagac cggcctcgcc tggtcggagg tcgcgcgcag caagctggga
     2821 gcgctccccc cgtccccgcg cctcgaggcc gacctgcaca gcgacctcgg cctgctgctg
     2881 caacaacgcg aggatctcgg cggttcgcgc gaagaacacc tggcccgcgc gcgtgaggag
     2941 cacggccggg cgctcgagct gcgcaagcag ctcgcggggg atcgagagac gcccgagctc
     3001 gccttcagct atctcaacct cggcgcgctc gaggtcagcc agggccggca cgagctcgcc
     3061 gtcgaggacc tccag
//
LOCUS       DASWNJ010000390         3807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_7654, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000390 DASWNJ010000000
VERSION     DASWNJ010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7654"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /gene="tssI"
                     /locus_tag="VGB85_06735"
     CDS             complement(<1..879)
                     /gene="tssI"
                     /locus_tag="VGB85_06735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HEY0133756.1"
                     /translation="MPFLRSLLGDQPLFTFEAAGGGELRVVRFYGQEGLSGLFEFHLE
                     LAGGDVDLAAIIDTPGVLKIDGLEAPRLVSGVISSFEYVGQSRNLQLYEAQLVPWIWR
                     LQHRHDCRIFQDRSTPDILREVLSIAGLSGEAFRFDLLGEYAPRNYCVQYRESDLAFI
                     SRLMEEDGIYYFFEHSADKHVLVMADHPGAHPPIPGSPALWFSPPGGMVADREHVQQF
                     RFGERVRPGKVSLRDFNLHKPGMPMEAAEAGKVRAELEVYQYPGEYQDPGQGGAHHGR
                     SQAKLRLEALQAYRRVG"
     gene            1084..2322
                     /locus_tag="VGB85_06740"
     CDS             1084..2322
                     /locus_tag="VGB85_06740"
                     /inference="COORDINATES: protein motif:HMM:NF042526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein"
                     /protein_id="HEY0133757.1"
                     /translation="MQITVIDHKARRTVCERLFDVFPIRIGRDAANDVVLPFAFVSSW
                     HAEVRSDGESLRLCDLGATNGLFIGPRRIAGGGAVPLTDRLVVTVGELELRLEVVRPD
                     APAEPAEADDLPRLPGASAPRGPVAASGSIDALPSFATDDTDFAARLARLQAALHELR
                     PHHAQLEAARRAWDERLAHSVRALEASGDRQGVNMLLREFPARDRGGFVIAGATDPFA
                     AVEEERSAVMQAASELLPGQRAPTGDDETRRFLTRIIDVLRVFAACTLELQHIRALQA
                     EELGVTWADPNDPLAALATADEVLRYLLDWRDTGEKRSEELVRVFGGLVDHHRAYVRS
                     ALEAARSALAALAPTEIERGAVTGWPGRAAALWHHYESVYAALHSDTHDHLTPAFRAT
                     LQRAYTQALARAGIAHHKAT"
     gene            2403..3242
                     /locus_tag="VGB85_06745"
     CDS             2403..3242
                     /locus_tag="VGB85_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133758.1"
                     /translation="MPVCPATLVGSDRALEADTRMLPPAQWLSLLVRGVDRSTMLPPE
                     DPHECSGLRIGLEWPDERSAVVDPRSDARPLPARPLTDADMTFGEGPDGQVLIWARIT
                     YFDDGTALGPVALARWVERGVEIRGIGTLWAPQRRARLRIEPLGEDAQLLVADGERCT
                     GEDLRSCIHEIYLLPLVNQRFTQAELVVDDTPAGPARVVSVERRDTPLPDGWVRRSDV
                     QRRLRLSGDSTVISELIRIRVCDPKNQPEMCQEEYRAEENRPLAWKDGRFTTSRSAWD
                     DTP"
     gene            complement(3263..>3807)
                     /locus_tag="VGB85_06750"
     CDS             complement(3263..>3807)
                     /locus_tag="VGB85_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133759.1"
                     /translation="ELQRVEDSAALTELRVYPSRKLRSYYPQLNDPEDYDVSGRKIKV
                     RGALRPHLRTLRRGTPGKVVQEQKLGGSRMFWVSFSRACKDAGCAYGFVQTEYDRWSL
                     VAVPRLETYKEPVSYRRNRLRRNRLRLGRQRAMNELNEVLVVPRRIGRAKTVDLQIFK
                     DVFRPTLKTRSREEGVEIPD"
BASE COUNT          583 a         1430 c         1251 g          543 t
ORIGIN      
        1 gccgaccctg cgatacgcct gcagcgcctc gagccggagc ttcgcctgcg accttccgtg
       61 gtgcgcgccg ccctgaccgg gatcctggta ctcgccggga tattggtaga cctcgagctc
      121 ggcccgcacc ttgccggcct ccgcggcctc catcggcatg ccgggcttgt gcaggttgaa
      181 gtcacgcagc gagaccttgc cgggccgcac gcgctcgccg aagcgaaact gctgcacgtg
      241 ctcccgatcg gcgaccatcc ctccgggcgg gctgaaccac agcgccggcg aaccggggat
      301 cggcgggtgc gcgccggggt ggtcggccat cacgagcacg tgcttgtcgg cgctgtgctc
      361 gaagaaatag tagatgccgt cctcctccat cagacggctg atgaacgcga gatccgactc
      421 gcgatactgc acacaatagt tcctcggggc atactcgccg agcaggtcga agcgaaacgc
      481 ctcccccgag aggcccgcga tgctcaggac ctcgcgcaaa atgtcggggg tgctccggtc
      541 ctgaaagatg cggcagtcgt ggcggtgctg caggcgccag atccacggca ccagctgcgc
      601 ctcgtacagc tggaggttgc gcgactggcc gacatattcg aacgaggaga tcacgccgga
      661 gacgaggcga ggcgcctcga ggccgtcgat cttcaggacc cccggcgtgt cgatgatcgc
      721 ggcgaggtcg acgtccccgc ccgcgagctc gaggtggaac tcgaacagcc ccgagagccc
      781 ctcctgcccg tagaagcgga cgacccgcag ctcgccgcct ccggcggcct cgaaggtgaa
      841 gagcggctgg tcgccaagga gcgagcggag gaacggcatc aagctcacgg gattgtcatc
      901 ctcaatcgct atcaagagag cacgaacggc aggtgtcaac aatccccaac gcgggttgtc
      961 ccgccctcca cgacaagccc caaagatact tgcccgatcg gcggcgtccc caatcgacgt
     1021 tggtgattgg cgcgccgcaa aaaagccgta tcgtacggag gtcgcgggcc aacgatgcgg
     1081 tcgatgcaga ttacggtgat cgatcacaag gcgcggcgga ccgtgtgcga gcgcctcttc
     1141 gacgtgttcc cgatccgcat cggccgtgac gccgccaacg acgtcgtcct gccgttcgcg
     1201 ttcgtctcgt cgtggcacgc cgaggtccgc agtgacggcg agtcgctgcg cctgtgcgac
     1261 ctcggcgcga ccaacggcct gttcatcggc ccccgccgga tcgccggcgg cggcgccgtg
     1321 ccgctgaccg accgcctcgt cgtcacggtc ggcgagctcg agctgcgcct cgaggtcgtt
     1381 cgccccgacg cccccgccga gcccgccgag gcggacgacc tcccgcgcct ccccggcgcg
     1441 tccgctccac gcggcccggt agcggcctcc ggcagcatcg acgccctccc gagcttcgcc
     1501 accgacgaca ccgacttcgc ggcccggctc gcccgcctcc aggccgcgct ccacgagctg
     1561 cgcccgcacc acgcccagct cgaggcggcc cgccgcgcgt gggacgagcg cctcgcacac
     1621 agcgtgcgcg cgctcgaggc cagcggcgac cgccagggcg tcaacatgct gctgcgcgag
     1681 ttcccggcgc gcgaccgcgg cggcttcgtc atcgccggcg cgaccgaccc gttcgcggcg
     1741 gtcgaagagg agcgcagcgc cgtcatgcag gcggccagcg agctgctgcc cggccagcgc
     1801 gccccgaccg gcgatgacga gacgcgcagg ttcctgacgc gcatcatcga tgtgctgcgg
     1861 gtgttcgccg cctgcacgct cgagctgcaa cacatccgcg ccctgcaggc cgaggagctc
     1921 ggcgtcacct gggccgaccc gaacgacccg ctcgccgcgc tggcgaccgc cgacgaggta
     1981 ttacgatacc tgctcgactg gcgggacacc ggggagaagc gcagcgagga gctcgtgcgc
     2041 gtgttcggtg ggctcgtcga ccatcaccgg gcctacgtcc gctcggccct cgaggccgcc
     2101 cgcagcgccc tcgcggcgct cgccccgacg gagatcgagc gcggcgcggt cacgggctgg
     2161 ccgggtcgcg ccgcggccct ctggcaccac tacgaatccg tgtacgcggc gctgcacagc
     2221 gatacccatg accacctgac gccggcgttt cgcgcgacct tgcagcgcgc ctatacccag
     2281 gccctcgccc gcgccggcat cgcccaccac aaggcaacat gacgcgcatt cgctcgctcc
     2341 tcgcaccccc attcctcgcg ctgctctgca ccgcgctgtc tgcctgccgc cccaccggca
     2401 cgatgcccgt gtgtcccgcg acgctcgtcg gcagcgaccg cgcgctcgag gccgacactc
     2461 gcatgttgcc gccggcccag tggctcagcc tgttggtccg cggcgtcgac cgctcgacga
     2521 tgttaccgcc cgaggatccc cacgagtgca gcgggctgcg catcggcctc gagtggcccg
     2581 acgagcgcag cgccgtggtc gacccccgca gcgatgccag gccgctgccg gcgcgcccac
     2641 tgaccgacgc cgacatgacc ttcggcgagg gcccggacgg gcaggtcctg atctgggccc
     2701 gcatcaccta cttcgacgac ggcaccgcgc tcggcccggt cgcgctcgct cgctgggtcg
     2761 agcgcggcgt cgagatccgc ggcatcggca cgctgtgggc cccccaacgg cgcgcccgcc
     2821 tccgcatcga gccgctcggc gaggacgccc agctgctcgt cgccgacggc gagcgctgca
     2881 ccggcgagga cctgcgcagc tgcatccacg agatctacct gctgccgctc gtcaatcaac
     2941 gcttcacgca ggccgagctc gtcgtcgacg acacgccggc cggtccggcc cgcgtcgtca
     3001 gcgtcgagcg ccgcgatacc ccgctcccgg acggatgggt ccgtcgctcc gacgtccagc
     3061 ggcgcctgcg cctcagcggc gacagcaccg tgatcagcga gctcattcgc atccgcgtgt
     3121 gcgacccgaa gaaccagccc gagatgtgcc aggaggagta ccgcgccgag gagaaccgcc
     3181 ccctggcctg gaaagacggc cggttcacga ccagccgcag cgcctgggac gatacgccct
     3241 gagcgtgatc accggatcat ggtcagtccg ggatctccac gccctcttcc cggcttcggg
     3301 tcttgagggt cggccggaac acgtccttga agatctggag gtcgaccgtc ttggcgcgac
     3361 cgatccgccg cggcacgacg agcacctcgt tgagctcgtt catcgcccgc tgtcggccca
     3421 ggcgcagacg attgcggcgc aggcgattgc ggcgataaga taccggctcc ttgtatgtct
     3481 ccagccgcgg cacggcgacg agcgaccagc gatcgtactc ggtctgcacg aagccgtacg
     3541 cgcagccggc gtccttgcaa gcgcgcgaaa agctgaccca gaacatgcgg ctacccccga
     3601 gcttctgctc ctgcacgacc ttgccggggg tccctcgccg gagcgtacgc aggtgcgggc
     3661 gcaaggcgcc gcgcaccttg atcttgcgac ccgagacgtc atagtcctcg gggtcgttga
     3721 gctgcggata atacgatcgc agcttgcggc tcggatacac ccgcagctcg gtcagcgcgg
     3781 cgctgtcctc gactcgttgc aactcgg
//
LOCUS       DASWNJ010000391         2470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_78634, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000391 DASWNJ010000000
VERSION     DASWNJ010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78634"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="VGB85_06755"
     CDS             complement(<1..1241)
                     /locus_tag="VGB85_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY0133760.1"
                     /translation="MFVAQRPRRVTPALLGLLAPLAGCGHHGRHGHGHGHADGHGSAS
                     ARAPVCVAETVTPKAPPTPAEALAAYVPAHYSKQELRIPMRDGVELFTAVYTPKDTSK
                     TWPILLRRTPYSCAPYGADAYPPALGPSAPFAEAGYIFVYQDVRGAFMSGGEFLDVRP
                     HNPNKAGPKDIDESSDTFDTIAWLIEHVPANNGRVGIYGISYPGFYAAAGMIDAHPAL
                     FAASPQAPIADWFFDDFHHHGAFFLPHSFNFYAGFGQPRPAPKTTWNKGFVHGTPDGY
                     QFFLDVGPLKNLDAKHLKGQIAFWNDLTSHPNYDEFWQARNLLPHLKKVAPAVLTVGG
                     WFDAEDLYGPLHIYEAVERQNPGITNLLVMGPWGHGGWSRTTGERMGDIVFGSPTSEH
                     YQKTIELPFFEHHLKGAATPGL"
     gene            1282..2424
                     /locus_tag="VGB85_06760"
     CDS             1282..2424
                     /locus_tag="VGB85_06760"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEY0133761.1"
                     /translation="MSVGILTSGGDAPGMNAAVRAAFRSLKMRAPDSELVLFRDGFRG
                     LARRLDSASDRSVDRKDVRDIIHRGGTFIGTGRVPELYMPKDDAPDREDRLAAREAFL
                     RVAAVNLFQTRVENLIVIGGDGSFRGARAIAAAYREMFPRRPFRIIGLPGTIDNDLHG
                     TEYSIGYDTALNNVVDAIRKLRDTIESHRRAIILEVMGNTSGWLALQSAIAGGAAAVA
                     IPEVEETWDQDDLVRRLVAGVRRDYRYFIIVVAEGVLKRAGADWSQRLARRIEEAAPI
                     QEILGHAMEARINSVGHIARGGSPSALDNTLAGLLGSAAADLAQLGALAHGPAVEGDV
                     MVGWHGTHVVVTPLDEVIENSPRLVTREGELYQLSNKLMLSAGQPF"
BASE COUNT          367 a          861 c          877 g          365 t
ORIGIN      
        1 agccctggcg tcgcggcgcc cttgaggtga tgctcgaaga acggcagctc gatcgtcttc
       61 tgatagtgct cggaggtcgg gctgccgaac acgatgtcgc ccatgcgctc gccggtggtc
      121 cgtgaccagc cgccgtggcc ccacgggccc atcaccagca ggttggtgat gccggggttc
      181 tggcgctcga ccgcctcata gatgtgaagg ggaccataga ggtcctcggc gtcgaaccag
      241 ccgccaaccg tcagcaccgc gggcgcgacc ttcttgaggt gcgggagcag gttacgcgcc
      301 tgccagaact cgtcgtaatt cgggtggctc gtcaggtcgt tccagaacgc gatctggccc
      361 ttgaggtgct tcgcgtcgag gttcttgagc ggcccgacgt cgaggaagaa ctggtagccg
      421 tcgggcgtgc cgtgcacgaa gcccttgttc cacgtggtct tcggggccgg ccggggctgc
      481 ccgaagccgg cgtagaagtt gaagctgtgc ggcaggaaga acgcgccgtg gtggtggaag
      541 tcgtcgaaga accagtcggc gatcggcgcc tgcgggctcg cggcgaacag cgccgggtgg
      601 gcgtcgatca tgcccgcggc ggcgtaaaac ccggggtacg agatcccgta gatgccgacc
      661 cggccgttgt tggccggcac gtgctcgatc agccaggcga tcgtgtcgaa ggtgtcgctc
      721 gactcgtcga tgtccttcgg gccggccttg ttggggttgt gcgggcggac atcgaggaac
      781 tcgccgccgg acatgaaggc gccgcggacg tcctgataaa cgaagatgta gccggcctcc
      841 gcgaacgggg cgctcgggcc gagcgcgggc gggtaggcgt ccgcgccgta gggcgcgcag
      901 ctgtagggcg tgcgccgcag caggatcggc caggtcttcg aggtgtcctt cggcgtgtag
      961 accgcggtga acagctcgac gccgtcgcgc atcgggatcc gcagctcctg cttgctgtag
     1021 tgcgcgggca cgtaggcggc gagcgcctcc gcgggcgtcg gcggcgcttt cggggtcacc
     1081 gtctcggcga cgcacaccgg cgcccgcgcg gacgcagacc cgtggccgtc ggcatggcca
     1141 tggccatgac catggcggcc gtggtgaccg catccggcca gcggggccag caggccgagg
     1201 agggcagggg tgacacgtcg cgggcgctgg gcaacgaaca ccgctccggt atactcccgc
     1261 ccccggagcc gaggccagcg aatgagcgta gggatcctga ccagtggggg cgacgccccc
     1321 ggaatgaacg cagcggtgcg cgccgcgttt cgcagcctca agatgcgcgc gcccgacagc
     1381 gagctcgtgc tgttccgcga cggctttcgt gggctcgccc gccgcctcga ctcggcctcg
     1441 gaccgcagcg tcgaccgcaa ggacgtccgc gacatcatcc accgcggcgg gacgttcatc
     1501 ggcaccggcc gtgtccccga gctgtatatg cccaaggatg acgcgcccga ccgcgaggac
     1561 cgcctcgccg cccgcgaggc gttcctgcgg gtcgcggccg tcaacctgtt ccagacccgc
     1621 gtcgagaacc tgatcgtcat cggcggcgac ggcagcttcc gcggcgcccg ggcgatcgcg
     1681 gcggcctacc gcgagatgtt cccgcggcga ccgtttcgca tcatcggcct gccgggcacg
     1741 atcgacaacg acctgcacgg caccgaatac tcgatcggct acgacaccgc cctcaacaac
     1801 gtcgtcgacg cgatccggaa gctgcgcgac acgatcgagt cgcaccgccg cgcgatcatc
     1861 ctcgaggtga tgggcaacac gtcgggctgg ctcgcgctgc agtcggcgat cgccggcggc
     1921 gcggcagccg tggcgatccc cgaggtcgag gagacctggg accaggacga cctcgtgcgc
     1981 cgcctcgtcg ccggcgtgcg tcgcgactac cgctacttca tcatcgtcgt cgccgagggc
     2041 gtgctcaagc gcgccggtgc cgactggtcg cagcggctcg cgcgtcgcat cgaggaggcc
     2101 gcaccgatcc aggagatcct cggccacgcg atggaggcgc gcatcaactc ggtcggccac
     2161 atcgcccgcg gcggcagccc ctcggccctc gacaacacgc tcgccggcct gctcggctcc
     2221 gccgccgccg acctcgccca gctcggcgcg ctcgcccacg gccccgccgt cgagggcgac
     2281 gtcatggtcg gctggcacgg cacccacgtc gtcgtcaccc ccctcgacga ggtgatcgag
     2341 aactcgccgc ggctcgtcac ccgcgagggc gagctctacc agctctcgaa caagctcatg
     2401 ctctcggcgg gccagccgtt ctgagggcga gcgatcgact ggccgcactc cctgccagtc
     2461 gaggcgagcg
//
LOCUS       DASWNJ010000392         2331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_537947, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000392 DASWNJ010000000
VERSION     DASWNJ010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537947"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VGB85_06765"
     CDS             complement(<1..684)
                     /locus_tag="VGB85_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 family protein"
                     /protein_id="HEY0133762.1"
                     /translation="MSAQRGDPSAEAIQGLHDRYLLEVVEDLSLCPFARRCREQGRLA
                     RPLFYAEPGESEPDAVARTLVALARAEPEVEVVLLTFVLPRATDHPWHDVDDFENYVR
                     AVRDAYEALRPSLRFYMVGFHPGLRGPDPRRPATPDGLVPRIRRTPDPVIQCIRADLL
                     DQVRRQAQVAANARFLEEMGKLGPEYRAMAMTAIQSDPELSADIARHNFENVGTGPGR
                     ATLEARLAEL"
     gene            712..1428
                     /locus_tag="VGB85_06770"
     CDS             712..1428
                     /locus_tag="VGB85_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0133763.1"
                     /translation="MRGKSVLLLCVSLSGCARYVVHVETDPGGAKVTLNGNTARGDRW
                     SVPHESGAEIVTTWPDGRRLATALLVDRDMRVVVRRDGDPSQVVGARPMLAAPAATPG
                     PAPMTPVPAPAPAPAAEEAGDPMTRAKGLAEAGQKAYELTDYEEAITRFKQAYELIRS
                     SKDPRAPEILGNILYNLAVVYERSHEVTPEVERLRRARVMYRQFDEQMAALVKDWAAS
                     AEHADVLSRIRALDARIGDK"
     gene            1525..>2331
                     /locus_tag="VGB85_06775"
     CDS             1525..>2331
                     /locus_tag="VGB85_06775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HEY0133764.1"
                     /translation="MFKNHPKGLPVLFFTEMWERFGFYLMLGIFVLYMRAGANGVFPA
                     KSGLGLDPATASDIYGTYIALVYLTPFVGGLLADRVLGYRRSIIIGGVLMGLGYCGLA
                     IPGYGVHFFLSLLFIIVGNGFFKPNISTLVGNLYNDERYKANKDSGFNIFYMGINIGA
                     LACNFVAAYLRINYGWGYAFLAAGIGMFIGVAWFLAGQKHTVHADILKPTMPEDQPVG
                     SILAQVFVPAFAFAAIGWFVPNLLLGSGVEGAEPYTFLGKQSNDAFLFACI"
BASE COUNT          349 a          751 c          855 g          376 t
ORIGIN      
        1 cagctcggcg aggcgggcct cgagggtggc gcggcccggg ccggtgccga cgttttcaaa
       61 attgtggcgg gcgatgtcgg cggacagctc ggggtcggac tggatcgcgg tcatggccat
      121 cgcgcggtac tcggggccga gcttgcccat ctcctcgagg aagcgggcgt tggcggcgac
      181 ctgggcctgg cggcgcacct gatcgagcag gtcggcgcgg atgcactgga tcaccgggtc
      241 gggggtccgg cggatccgcg ggaccaggcc gtcgggggtc gcaggccgcc gtggatccgg
      301 accgcgcagg ccggggtgga agccgaccat gtaaaagcgc agcgacggac gcagggcctc
      361 gtaggcgtcc cggacggcgc gcacgtaatt ctcgaagtcg tcgacgtcgt gccaggggtg
      421 gtcggtcgcg cgcgggagca cgaaggtcag cagcacgacc tcgacctcgg gctcggcccg
      481 agcgagcgcc acgagcgtgc gcgcgaccgc gtcgggctcg ctctcgccgg gctcggcgta
      541 gaagaggggc cgcgcgagcc gaccctgctc gcggcagcgg cgagcgaagg ggcacagcga
      601 gaggtcctcg acgacctcga gcaggtagcg atcgtggagg ccctggatgg cctctgcgga
      661 cggatcaccg cgctgggcag acatgcgggg cgcagtgtac attaggcggc gatgcgtgga
      721 aaaagcgtgc tgctcctgtg cgtcagcctg tcgggctgcg cgcgctacgt ggtgcacgtc
      781 gagaccgacc cgggcggcgc gaaggtgacg ctcaacggca atacggcccg cggtgaccgt
      841 tggtccgtgc cccacgagtc cggggcggag atcgtcacga cctggccgga tggtcggcgg
      901 ctggcgaccg cgttgctggt cgaccgcgac atgcgcgtgg tggtgcgccg cgacggcgac
      961 ccgtcgcagg tggtcggcgc gcgcccgatg ctggccgcgc cggcggcgac gcccggaccg
     1021 gcgccgatga cacctgtccc tgcacctgcg ccggcgccgg cggccgagga ggcgggcgac
     1081 ccgatgacac gggccaaggg attggccgag gccggtcaga aggcctacga gctgaccgac
     1141 tacgaggagg cgatcactcg cttcaagcag gcctacgagc tgatccgcag cagcaaggat
     1201 ccccgggcgc cggagatcct cggcaatatc ctctacaacc tcgcggtggt gtacgagcgc
     1261 agccacgagg tcacgccgga ggtcgagcgc ctgcgacgtg cccgcgtcat gtaccggcaa
     1321 ttcgacgagc agatggccgc gctggtcaag gactgggccg cctccgcaga gcacgccgac
     1381 gtcctgagcc gcatccgtgc gctcgacgcc cgtatcggcg acaagtagac gcgacttccc
     1441 tcgcggcgaa aacggccgta tcgtggtccc tcgcgggcgc cggacctgtt cggttgttca
     1501 gcgcgtgtcg gccgaggaac gctgatgttc aagaaccatc ccaagggtct gcctgtcctg
     1561 ttcttcaccg agatgtggga gcgcttcggc ttctacctga tgctcgggat cttcgtgctc
     1621 tacatgcgcg cgggcgcgaa cggggtgttc ccggcgaaga gcgggctcgg cctcgaccct
     1681 gcgacggcct cggacatcta cggcacgtac atcgcgctgg tgtacctgac gccgtttgtc
     1741 ggtggtttgc tggcggaccg cgtcctcggg tatcgccggt cgatcatcat cggcggggtg
     1801 ctgatggggc tcggctactg cgggctggcg atccccgggt acggggtgca cttcttcctg
     1861 tcgctgctgt ttatcatcgt cggcaacggc ttcttcaagc cgaacatctc gacgctggtc
     1921 gggaacctct acaacgacga gcgttacaag gccaacaagg actccggctt caacatcttc
     1981 tacatgggca tcaacatcgg tgccctcgcc tgcaacttcg tggcggcgta cctgcggatc
     2041 aactacgggt ggggctacgc gttcctggcc gcgggcatcg gcatgttcat cggcgtcgcg
     2101 tggttcctgg ccggccagaa gcacacggtg cacgccgaca tcttgaagcc gacgatgccg
     2161 gaggaccagc cggtcgggtc gatcctcgcg caggtgttcg tgccggcgtt cgcgttcgcg
     2221 gcgatcgggt ggttcgtgcc gaacctgctg ctcgggtccg gggtcgaggg cgccgagccg
     2281 tacaccttcc tcggcaagca gtcgaacgac gcgttcctgt tcgcgtgcat c
//
LOCUS       DASWNJ010000393         2307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_322785, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000393 DASWNJ010000000
VERSION     DASWNJ010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322785"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2307
                     /locus_tag="VGB85_06780"
     CDS             <1..>2307
                     /locus_tag="VGB85_06780"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0133765.1"
                     /translation="DVAHVHDGYAIQTNIARRDGRRSVVLAVMKTGDASTTDVAERVK
                     ALVPTIQAAAPKGLEVELLSDQSQFVIRAIDGLLIEGLIATLLTATMILLFLGSFRAT
                     ILVVVNIPLAVMISLLCIHALGGTINVMTLGGLALAVGILVDDATVEIENIHRNLAMG
                     KTLTRAILDGAQQIAVPAFVASLCISIVFVSVVFLEGPARFIFLPMGMAVAFSVMASY
                     LLSRTLVPTLAMYLLRGHADHAKDAAAPSRGLFARMHRGFEAGFEALRARYVELLEWA
                     LHHRKTVFAAFGVAIVASAALVPFVGRDFFPQVDAGQLRLHVTAPPGTRIEETERWFS
                     RVEDHIRAIVPEEDRESILDFIGIPSGYNLSITDSSNVGSTDGEILVTLAHERTSKTA
                     DIMRALRAELPREFPELSFYFQPADIVTQILNFGLPSPIDLQVSGAQRDVTLATARKL
                     EAELRTVPGVVDAHLHQIVAAPRLHVDVDRLRASEAGLTERDVAANLLLMVSSSTQVS
                     PTFWTDSKTGLAYPVALQVPEHRVDSIDALAGLSMATEDGRQKLLVDVADIERATTPV
                     FVSHANVQPAYNVRADVQDSDLGTAVARIEEIVARHRAELPPGAAITVRGQAESMQAG
                     FRGLALGLGFAALLVYALMVINFQSLKLPLIILMALPGAGVGIVFTLFATGTTFSIPS
                     LMGAVMSIGVATANSILVVTFANEQRVHGSSALAAALEAGSVRLRPVLMTAMAMFIGM
                     LPMSLGLGEGGEQNAALGRAVMGGLAGAT"
BASE COUNT          324 a          806 c          807 g          370 t
ORIGIN      
        1 gacgtcgccc acgtgcacga tggctacgcg atccagacca acatcgcccg ccgcgacggc
       61 cgccggtcgg tcgtgctcgc ggttatgaag accggcgacg cctcgaccac cgacgtcgcc
      121 gagcgcgtca aggcgctggt gccgacgatc caggccgccg cgccgaaggg cctcgaggtc
      181 gagctgctgt cggaccagtc gcagttcgtg atccgcgcga tcgacggcct gttgatcgag
      241 ggcctgatcg ccacgctgct gacggcgacg atgatcctcc tgtttttggg cagctttcgc
      301 gcgacgatcc tcgtcgtcgt caacatcccg ctcgcggtga tgatctcgct gctgtgcatc
      361 cacgccctcg gcggcacgat caacgtgatg accctcggcg ggctcgcgct cgcggtcggc
      421 atcctcgtcg acgacgcgac ggtcgagatc gagaacatcc accgcaacct ggcgatgggc
      481 aagacgctga cccgcgcgat cctcgacggc gcccagcaga tcgcggtgcc cgcgttcgtc
      541 gcgtcgctgt gcatcagcat cgtgttcgtg tcggtcgtgt tcctcgaggg cccggcgcgc
      601 ttcatcttcc tgccgatggg catggcggtc gcgttctcgg tgatggcctc gtacctgctg
      661 tcgcgcaccc tggtgccgac gctggcgatg tacctgctgc gcggccacgc ggaccatgcg
      721 aaggacgccg cggccccgtc gcgcgggctg ttcgcgcgga tgcaccgcgg cttcgaggcc
      781 ggcttcgagg cgctacgcgc ccgctacgtc gagctgctcg agtgggcgct gcaccatcgc
      841 aagaccgtgt tcgcggcgtt cggcgtggcg atcgtggcga gcgcggcgct ggtgcccttt
      901 gtcggccgcg acttcttccc gcaggtcgac gccggccagc tgcgcctgca cgtgacggcc
      961 ccaccgggga cgaggatcga agagaccgag cggtggttta gccgcgtcga ggaccacatc
     1021 cgcgcgatcg tgccggagga ggaccgcgag agcatcctcg actttatcgg catcccgagc
     1081 ggctacaacc tctcgatcac cgacagcagc aacgtcggct cgaccgacgg cgagatcctg
     1141 gtgacgctgg cgcacgagcg taccagcaag acggcggaca tcatgcgggc gctgcgggcc
     1201 gagctgccgc gcgagttccc ggagctgtcg ttttacttcc agccggccga catcgtcacg
     1261 cagatcctca acttcggttt gccgtcgccg atcgacctcc aggtgtccgg ggcgcagcgc
     1321 gacgtgacgc tggcgaccgc gcgcaagctc gaggccgagc tgcgcacggt gccgggggtg
     1381 gtcgacgcgc acctgcatca gatcgtcgcg gcgccgcggc tgcacgtcga cgtcgaccgc
     1441 ctacgcgcca gcgaggccgg gctgaccgag cgcgacgtcg cggccaacct gctgctgatg
     1501 gtctcgtcgt cgacgcaggt cagcccgacg ttttggaccg acagcaagac cggcctggca
     1561 tatccggtcg ccttgcaggt gcccgagcac cgggtcgact cgatcgacgc gctcgccggg
     1621 ctgtcgatgg cgaccgagga cggccggcag aagctgctcg tcgacgtcgc cgacatcgag
     1681 cgcgcgacca cgccggtgtt cgtcagccac gcgaacgtgc aaccggccta caacgtgcgc
     1741 gccgacgtgc aggactcgga cctcggcacc gcggtggcga ggatcgagga gatcgtcgcc
     1801 aggcaccgcg ccgagctgcc gccgggcgcg gcgatcacgg tgcgcgggca ggccgagagc
     1861 atgcaggccg gctttcgcgg gctcgccctc gggctcggct tcgcggcgct gctggtctac
     1921 gcgctgatgg tgatcaactt ccagtcgctc aagctgccgc tgatcatcct catggcgctg
     1981 ccgggcgcgg gggtcggcat cgtgttcacg ctgttcgcca ccggcacgac cttcagcatc
     2041 ccctcgctga tgggcgccgt gatgagcatc ggcgtggcga ctgcgaactc gatcctcgtc
     2101 gtcaccttcg ccaacgagca gcgggtgcac gggagctcgg ccctcgcggc cgcgctcgag
     2161 gccggcagcg tgcgcctgcg gccggtgctg atgacggcga tggcgatgtt catcggcatg
     2221 ctgccgatgt cgctcgggct cggcgagggc ggcgagcaaa atgccgcgct cggccgcgcc
     2281 gtgatgggcg ggctcgccgg cgcgacg
//
LOCUS       DASWNJ010000394         2917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_113097, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000394 DASWNJ010000000
VERSION     DASWNJ010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113097"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="VGB85_06785"
     CDS             complement(<1..1110)
                     /locus_tag="VGB85_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133766.1"
                     /translation="MWSAPPRVLAGLIAGITAAGCHADPGDDTGGPEPSDTTGDPVPV
                     PSFLNPAVGSFNVASNQSEPETLVVQNILPGVTQVLLDGYSLGALDSPNPLGSLAPDL
                     LTLNIQGALVTGSHTLQLLTASPIGPLYSVELEMKVAAPEHATRPLWTAELDPTVVAT
                     GTVLFASGVGSGGLLGLVAPGVPDPELRLYAAGASGWTTTDPVVLPLVGHVLQDMSFE
                     PAISAVAFAAPNGGPPRRMRVVYNLGLPGVAIAARDIQIDPSPIVLDPVIAFDLPTAL
                     GDAQLEYAAFGRPVALGDSVVAELHAAADAEQPHPGDHRLIASFWRGEALGWTPPQQI
                     GTAAPSDLDALGPAPVLADIPAGATTTLSVRLGGAF"
     gene            complement(1163..2077)
                     /locus_tag="VGB85_06790"
     CDS             complement(1163..2077)
                     /locus_tag="VGB85_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133767.1"
                     /translation="MNPPPRNRRRQHPSNRRRPRPSSTHVAARRHSRWLAMQLQTAGV
                     WLRGLPARLRSAAETTVSNRALRPWASEPPPAELRESAELYAAVDTGPQIVLDDVPAT
                     PAPKRTPRPFQRAGELSKQLAPRAAALGRQLAPQAAAFGRQLGPLARRGAAATGQGLR
                     ATGRRLADLRPLLRLLGRFLATILAGIGGLTVRVLGALARGSTITGDRIAGALQRNQA
                     VLFALFVRAMWWTALALLFLGGRALVEIHDHAPFVQQALPVFLSGLAMCAFLLIVASP
                     ARLRWAAFALGIGHGGLLALVWVVSAIG"
     gene            2158..>2917
                     /locus_tag="VGB85_06795"
     CDS             2158..>2917
                     /locus_tag="VGB85_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133768.1"
                     /translation="MPLGSLVLVSALLAAPAEGPAPTVWAEAGGRPWTTCRERERQAE
                     TVLQRVSTRNEDSGDSPWFAAARECPHAPAVLVVAAMRALANVPAYPMGGELQESLPK
                     LAAAHRIGRKQARSWLRTALAEAQRRGDPAPVLTHYFLAQAALGLGEPVTARAELATA
                     VARGEVEAWRADHALAVAALLEGNLEAALDLAYRARELAPASERTTSTFLLALVYDRS
                     GAPEAALREMLALGSREGERGAADINLPLHERLYL"
BASE COUNT          373 a          944 c         1264 g          336 t
ORIGIN      
        1 gaaggcgccg ccgaggcgca cggacagggt ggtggtcgcg ccggccggga tgtccgcgag
       61 gacaggtgcg ggtccgaggg cgtcgaggtc gctcggggcg gcggtgccga tctgctgcgg
      121 cggggtccag ccgagcgcct cgccgcgcca gaagctggcg atcaggcggt ggtcgccggg
      181 gtgcggctgc tcggcgtcgg ccgcggcgtg cagctcggcg acgacgctgt caccgagcgc
      241 gaccggacgg ccgaaggcgg cgtactcgag ctgggcgtcg ccgagggcgg tcgggaggtc
      301 gaaggcgatg accgggtcga gcacgatcgg gctcgggtcg atctggatgt cgcgggcggc
      361 gatcgccacg cccggcagac cgaggttgta gacgacgcgc atgcggcggg gcgggccgcc
      421 gttcggcgcc gcgaaggcga cggcggagat ggctggctcg aaggacatgt cctgcaggac
      481 atgtcccacg agcgggagca cgaccgggtc ggtggtcgtc cagccgctgg cgccggcggc
      541 gtagaggcgc agctcggggt cgggcacgcc gggggcgacg aggccgagca ggccgccgct
      601 gccgaccccg gaggcgaaca ggacggtgcc ggtggcgacg acggtcgggt cgagctcggc
      661 ggtccacagc ggccgggtcg cgtgctcggg cgccgcgacc ttcatctcga gctcgacgga
      721 gtagagcggg ccgatcgggc tcgcggtcaa cagctgcagg gtgtggctgc cggtgacgag
      781 ggcgccctga atgttgagcg tcagcaggtc gggcgcgagg ctgccgagcg ggttggggct
      841 gtcgagcgcg ccgagcgagt aaccatcgag caggacctgg gtgacgcccg ggaggatgtt
      901 ctggacgacg agggtctcgg gctcgctctg gttactcgca acgttgaagc tgccgacggc
      961 cgggttgagg aacgacggga ccgggacggg gtcgccggtg gtgtcgctgg gctcggggcc
     1021 gccggtgtcg tcgccgggat cggcgtggca gccggccgcc gtgatgccgg cgatcaggcc
     1081 ggcgaggacg cgcggcgggg cagaccacat gcctcccagg gtacgcgcgg cgggccggac
     1141 ccgccatcgg tcatgggacg ggtcagccga tggcgctgac gacccagacc agcgcgagca
     1201 ggccgccatg gccgatgccg agagcaaagg cggcccagcg caggcgggcc gggctggcga
     1261 cgatgagcag gaacgcgcac atcgcgaggc cgctgaggaa caccgggagg gcctgctgca
     1321 cgaagggggc gtggtcgtgg atctcgacga gggcgcggcc gccgaggaac agcagggcga
     1381 gcgcggtcca ccacatcgcc cggacgaaca gcgcgaacag gacggcctgg ttacgctgca
     1441 gggcgccggc gatgcggtcc ccggtgatgg tgctgccgcg ggcgagggcg ccgaggaccc
     1501 gcacagtcag gccgccgatg ccggcgagga tcgtggcgag gaagcggccg agcaggcgta
     1561 gcagggggcg caggtcggcg aggcggcggc cggtcgcgcg cagaccctgg ccggtcgcgg
     1621 cggcgccgcg gcgggcgagc gggccgagct gtcggccgaa cgcggccgcc tgtggcgcga
     1681 gttgccggcc gagcgcggcc gcccgcggcg cgagctgctt gctgagctcg ccggcgcgct
     1741 ggaacggccg cggggtgcgc ttcggggcgg gcgtcgcggg gacgtcgtcg aggacgatct
     1801 gcgggccggt gtcgaccgcg gcgtagagct ccgccgactc gcgcagctcg gcgggcggcg
     1861 gctcggaggc ccacgggcgc agggcgcggt tggacacggt cgtctcggcg gcgctgcgca
     1921 ggcgggccgg caaaccacgc agccacacgc cggccgtttg cagctgcatg gcgagccagc
     1981 gcgagtgccg gcgggccgcg acgtgggtcg aggagggccg cgggcggcga cggttgctcg
     2041 ggtgctggcg acggcggttg cgcggcggcg ggttcacggc gcgaaggttt catgcgcggg
     2101 gcccggggcc aaatttttgc ctgtttgcgc ggggtatgca gcgctacgat cgccgcgatg
     2161 ccgctgggat cgctggtgct cgtgtcggcg ctgctggccg cccccgccga ggggccggcg
     2221 ccgaccgtgt gggcggaggc cggcggccgc ccgtggacga cctgccgcga gcgcgagcgc
     2281 caggccgaga cggtgctgca gcgggtcagc acgcgcaacg aggacagcgg cgactcgccg
     2341 tggttcgcgg cggcccgcga gtgcccgcac gcgccggcgg tgctggtggt cgcggcgatg
     2401 cgcgctctgg cgaacgtgcc ggcgtacccg atgggtggcg agctgcaaga gagcttgccg
     2461 aagctggccg cggcgcaccg catcggccgc aagcaggccc ggagctggct gcgtaccgcg
     2521 ctggcggagg cacaacggcg cggagatcca gcgccggtgt tgacgcacta cttcctggcc
     2581 caggcggcgc tcgggctcgg ggagccggtg acggcgcggg ccgagctggc gacggcggtc
     2641 gcgcgcggcg aggtcgaggc gtggcgcgcc gaccatgcgc tggccgtcgc ggcgctgctc
     2701 gagggcaacc tcgaggcggc gctcgacctg gcgtatcgcg cccgcgagct ggccccggcg
     2761 agcgagcgca cgacctcgac cttcctgctg gcgctggtct acgaccgctc gggcgcgccg
     2821 gaggcggcgc tgcgcgagat gttggcgctc ggctcccggg agggcgagcg cggcgcggcc
     2881 gacatcaact tgccgctgca cgaacggctg tacctcg
//
LOCUS       DASWNJ010000395         1539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_7679, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000395 DASWNJ010000000
VERSION     DASWNJ010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7679"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..255)
                     /locus_tag="VGB85_06800"
     CDS             complement(46..255)
                     /locus_tag="VGB85_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133769.1"
                     /translation="MRHDAPLRLRHLGCGEGYGRFYGSIVMFSAAVVAFIPAVVYGVR
                     LNRHNNTRPSTARLQLAPGDFALRF"
     gene            complement(271..>1539)
                     /locus_tag="VGB85_06805"
     CDS             complement(271..>1539)
                     /locus_tag="VGB85_06805"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0133770.1"
                     /translation="QQRQLDLALAHEAREEAVEAAMLGGDELAISDRPGPAANDVGPL
                     TKYAVIYGPSGEVLAQTPTWQGRPPPLSELPAPGTLAFDLWARDEHLRGIHVAIPAHP
                     DATMLLAAPRSDLDGDERFLMRAMGTVFGLALLWTVLVAVVVVWRLTRDHRTIAAVAR
                     RVVAGDIDARIGMHSGDREVMQLAQDIDAMIDHLGVLIGLQQRFIANAAHELRSPLTT
                     LYGELSHALRRSREPAEYERAIHEALTSTQRLRRLTEDLLAVARVAADPDATEEVIEL
                     EALCQEAVALVAAEAEARTVRIAWRGGDARTRGRRGDLLRLLRNLLENAVRHSPAGGQ
                     VHVHCRDLDGRIELTVRDEGPGVAPADRERIFEAFYRGARERAANDGGTGLGLTIARE
                     IARAHGGDLRLLEQASTGACFSLTIRAATS"
BASE COUNT          213 a          536 c          583 g          207 t
ORIGIN      
        1 cgtggcgccg cacgcgatcg tgcagcgccc cgggtggtgt cgctatcaga agcgcagcgc
       61 gaagtcaccc ggcgccagct gcaggcgtgc ggtgctcggg cgcgtattgt tgtgacgatt
      121 gagccgcacg ccgtagacga ccgccgggat gaacgcgacg acggccgcgg agaacatcac
      181 gatcgatccg tagaagcggc cataaccctc gccgcagccc aagtgccgca gccgaagtgg
      241 cgcatcgtgg cgcatcgtgg cgagcgacgc tcacgaggtc gcggcgcgga tcgtgagcga
      301 gaagcaggcg ccggtcgacg cttgctcgag caggcgcagg tcaccgccat gcgcccgcgc
      361 gatctctcgc gcgatcgtca ggccgaggcc ggtcccgccg tcgttcgcgg cccgctcccg
      421 ggcaccgcga taaaatgcct cgaagatccg ctcgcggtcg gccggcgcga caccaggccc
      481 ctcgtcgcgg acggtcagct cgatgcgtcc gtcgagatcc cggcaatgca cgtgcacctg
      541 gccacccgcg ggagagtggc ggaccgcgtt ttcgaggagg tttcgcagca gccgcagcag
      601 gtcgccgcgg cgtccccggg tccgcgcgtc gccacctcgc caggcgatcc ggaccgtccg
      661 cgcctcggcc tcggcggcga cgagcgcgac cgcttcctga cacagcgcct cgagctcgat
      721 gacctcctcg gtggcgtcgg gatcggcggc gacgcgggcc accgcgagca agtcctcggt
      781 cagccgccgc aaccgctggg tcgaggtgag cgcctcatgg atcgcgcgct cgtactcggc
      841 gggctctcgc gagcgccgca gggcgtgcga cagctcgccg tacagggtcg tcagcggcga
      901 gcgcagctcg tgggccgcgt tggcgatgaa gcgctgctgg aggccgatca gcacgccgag
      961 gtggtcgatc atcgcgtcga tgtcctgggc gagctgcatg acctcgcggt cgccgctgtg
     1021 catgccgatc cgggcgtcga tgtcgccggc gacgacgcgc cgggcgaccg ccgcgatcgt
     1081 tcggtggtct cgcgtgagcc gccagacgac gacgaccgcg acgagcacgg tccacagcag
     1141 ggcgaggccg aagaccgtgc ccatggcgcg catcaggaac cgctcgtcgc cgtcgaggtc
     1201 gctgcgcggc gcggcgagca gcatcgtcgc gtccgggtgc gccgggatag cgacgtggat
     1261 gccgcgcagg tgctcgtccc gcgcccagag gtcgaaggcg agggtgcccg gcgcgggcag
     1321 ctcggacagc gggggtggcc gcccttgcca cgtgggggtc tgggcgagga cctcgccgga
     1381 aggaccgtag atgaccgcgt acttggtcag ggggccgacg tcgttggcgg cggggccggg
     1441 tcggtcgctg atcgcgagct cgtcgccgcc gagcatcgcg gcctcgaccg cctcctcgcg
     1501 ggcctcgtgc gcgagcgcga ggtcgagctg gcgctgctg
//
LOCUS       DASWNJ010000396         3893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_183809, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000396 DASWNJ010000000
VERSION     DASWNJ010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183809"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VGB85_06810"
     CDS             <1..274
                     /locus_tag="VGB85_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133771.1"
                     /translation="TLFADGASEYAAVTCYKPQEMFIVDLSGVRVVANLLLGTGPHAM
                     TVDRARQYLYVANSLDKTISVVDISRLRPTRFSEVARLGRQVPYLR"
     gene            280..>3893
                     /locus_tag="VGB85_06815"
     CDS             280..>3893
                     /locus_tag="VGB85_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133772.1"
                     /translation="MPPKPFVLRHAALAAAFASLATAASLASLACNNRQSIIVPNRVL
                     DRPTDMVMACLRRDATTDIISPTSLDQCINATCGDLRLVGFIANSERDDIAMFSKCAN
                     SVLDMDEATPGAQLIPAGKVPSSMTITSGATNGCFAVSANLGSCDLSVLDVPGLSAYA
                     FDDVPVRDPAELVLAVTPTRGDGTPLGARPGQVIAAPPELSNSVLFGDLEGETAGDDG
                     AGDTGDTDTDGTVQVAACDLERPGSVYVTFPSCQLIAEVDLRTQRILQSRQFSRDPGT
                     GEVTVKDTGVDPSCPIDCPELYANNPGALTDAPPGEVDGMYPSAMALISPPVDPQDDA
                     DLQIVDHALYVGGLGSDTLFELRYDGRQWLTDPLALELQDASGISAIRPTPAMVLGLD
                     GAEPYHQFLYLIAGDGSTRVVRRDLDENRSEIGRECDTQVDPTAVLDLVCHPAEFPGE
                     NPPDRRPFARGPGIRGPNGSLITDWTFQKIARPVNGALPEAGSARSPFGKAGVTGVGT
                     TSFGRLVFVTFDQFNTDRAGNPTATRVSTTKDPLGLLDATIQPHMLWPTLDPVAEPVE
                     NTALPRMEDAVPQRLVPGDSEGPSALKVLSPALRRIDLAYSELGVACKYVADDQSEGT
                     ADDATCVEETGDKTAFCATDNQCRGQENPLLSPHILRDEGDRFGNPDIDEAGSNGLYK
                     NEVVRAVVRDYRSWIGGAWTLFWEGEIPDTASFNGQLICDHPGWEGGTCLSTEPGDTR
                     IVDSTARFCDAGVLAGDKLTLFGCGSDADCGVGQFCLVDPRTPTNGTGICVSQSAYDQ
                     QEDLRKICEDLIYDPCGSPVREFLITGAFQNELWLQAMDRPSTAYLMYSDAAISEEAG
                     DTDTDTGGDTGDMTISEPALCDGSYLRALPEDAPDPGTYRHECEARLLCAPEQPETGC
                     ETHDDCIALIEEGAAPEDSYPLCIDGLCRRPCQDGEDCVLRRLPGPTCMQELVRYLVQ
                     ARNSFVVRGPGAYDFLDQRVRADDNGACYEDPTVSNLMTSRIRLGRDEADSRNNAAWP
                     IPSCPPGSERPGAGTPNPCFIDTPRPESLTSDAPEGLFHYFDYGYGLEDSPVPAIRFS
                     NPMMSLVLDLTSLQGLVDPVPGTEDDVWDPKFRAFRRARIPRSFNESFSTVPGYAPFD
                     IGVITSSIALVGPTRIINAPEPGIVFIVDTAGGTGTGGVRGQVVRVGLVGGQQSPDEK
                     "
BASE COUNT          595 a         1561 c         1217 g          520 t
ORIGIN      
        1 gaccctgttc gccgacggcg ccagcgagta cgccgcggtc acctgctaca agccgcagga
       61 gatgttcatc gtcgacctca gcggcgtgcg cgtggtcgcc aacctgctgc tcggcaccgg
      121 cccccacgcg atgaccgtcg atcgcgcgcg gcagtacctc tacgtcgcca actccctcga
      181 caagacgatc agcgtcgtcg acatcagccg cctgcgtccg acccgcttct cggaggtcgc
      241 gcgcctcggt cgccaagtgc cttacctccg atgagctccg tgccacccaa accgttcgtc
      301 ctccggcacg ccgccctcgc cgcggccttc gcgtcgctcg cgaccgccgc gtcgctggca
      361 tcgctcgcgt gcaacaaccg ccagagcatc atcgtcccca accgcgtcct cgaccgcccg
      421 accgacatgg tcatggcctg cctgcggcgc gacgcgacca ccgacatcat cagcccgacc
      481 tcgctcgacc agtgcatcaa cgcgacctgc ggtgacctgc gcctggtcgg cttcatcgcg
      541 aattccgagc gcgacgacat cgcgatgttc agcaagtgcg ccaactcggt cctcgacatg
      601 gacgaggcca ccccgggcgc ccagctgatc cccgccggca aggtcccgtc gagcatgacg
      661 atcacctccg gggccaccaa cggctgcttc gccgtgtcgg ccaacctcgg cagctgcgac
      721 ctcagcgtgc tcgacgtccc cggcctgtcc gcctacgcct tcgacgacgt gcccgtccgc
      781 gacccggccg agctcgtgct cgccgtcacg ccgacccgcg gcgacggcac cccgctcggc
      841 gcccgacccg gccaggtgat cgcggccccg cccgagctca gcaacagcgt gctgttcggc
      901 gacctcgagg gcgagaccgc cggcgacgac ggggcagggg acaccggcga caccgacacc
      961 gacggcaccg tgcaagtggc cgcctgcgac ctcgagcgcc cgggcagcgt ctacgtcacg
     1021 ttcccgtcgt gccagctgat cgccgaggtc gacctgcgga cccagcgaat tctccagagc
     1081 cgccagttct cgcgcgatcc ggggaccggc gaggtcaccg tcaaggacac cggcgtcgac
     1141 ccctcgtgcc cgatcgactg cccggagctg tacgccaaca accccggcgc gctgacggac
     1201 gccccgcccg gcgaggtcga cggcatgtat ccctcggcga tggcgctgat cagcccgccc
     1261 gtcgacccgc aggacgacgc cgacctgcag atcgtcgacc acgccctgta cgtcggcggc
     1321 ctcgggtccg acaccctgtt cgagctgcgc tacgacggcc gccagtggct caccgacccg
     1381 ctcgcgctcg agctgcagga cgccagcggc atctctgcga tccgcccgac ccccgcgatg
     1441 gtgctcggcc tcgacggcgc cgagccctat caccagttcc tctacctgat cgccggcgac
     1501 ggctcgaccc gcgtcgttcg ccgcgacctc gacgagaacc gcagcgagat cggccgcgag
     1561 tgtgacaccc aggtcgaccc gaccgccgtg ctcgacctcg tgtgccaccc ggccgagttc
     1621 cccggcgaga acccccccga ccgccgcccc ttcgcccgcg gccccggcat ccgcggcccc
     1681 aacggctcgc tgatcaccga ctggaccttc cagaagatcg ccaggccggt caacggcgcg
     1741 ctgccggagg cgggcagcgc ccgctcgccc ttcggcaagg ccggcgtcac cggcgttggc
     1801 accacctcct tcggtcgcct ggtgttcgtc accttcgatc agttcaacac cgaccgcgcc
     1861 ggcaacccga ccgcgacccg cgtgtcgacg accaaggacc cgctcggcct gctcgacgcg
     1921 acgatccagc cgcacatgct ctggccgacg ctcgacccgg tcgccgagcc ggtcgagaac
     1981 accgccctgc cgcggatgga ggacgccgtt ccgcagcgcc tggtgcccgg cgacagcgag
     2041 ggtccctccg cgctcaaggt gctgtccccg gcgctgcgcc gcatcgacct cgcgtactcc
     2101 gagctcggcg tcgcgtgcaa gtacgtcgcc gacgaccagt ccgagggcac cgccgacgac
     2161 gcgacctgcg tcgaggagac cggcgacaag acggccttct gcgcgaccga caaccagtgc
     2221 cgcgggcagg agaacccgct gctctcgccg cacatcctcc gcgacgaggg cgaccgcttc
     2281 ggcaaccccg acatcgacga ggccggcagc aacggcctgt acaagaacga ggtcgtgcgc
     2341 gcggtcgtcc gcgactaccg cagctggatc ggcggcgcct ggaccctgtt ctgggagggc
     2401 gagatccccg acaccgcgag cttcaacggc cagctgatct gcgaccaccc cggctgggag
     2461 ggcggcactt gcctcagcac cgagcccggc gacacccgca tcgtcgacag caccgcccgc
     2521 ttctgcgacg ccggcgtgct cgcgggcgac aagctgaccc tgttcggctg cggctcggac
     2581 gcggactgcg gcgtcggtca gttctgcctc gtcgacccgc gcacgccgac caacggcacc
     2641 gggatctgcg tctcgcagag cgcctacgac cagcaggaag atcttcgcaa gatctgcgag
     2701 gacctgatct acgacccctg cggctcaccc gtgcgcgagt tcctgatcac cggcgcgttc
     2761 cagaacgagc tgtggctgca ggcgatggac cggccctcga ccgcgtacct gatgtacagc
     2821 gacgcggcga tctccgagga agccggcgac acggacacgg acaccggcgg cgacaccggg
     2881 gacatgacga tctccgagcc cgcgctgtgt gacggctcgt acctgcgggc cctgcccgag
     2941 gacgccccgg accccggcac ctaccgccac gagtgcgagg ctcgcctgct gtgcgcgccc
     3001 gagcagcccg agaccggctg cgagacccac gacgactgca tcgcgctgat cgaggagggc
     3061 gccgcccccg aggacagcta cccgctgtgc atcgacggcc tgtgccggcg cccctgccag
     3121 gacggcgagg attgcgtgct gcgccgcctg cccgggccga cctgcatgca ggagctggtc
     3181 cgctacctcg tccaggcgcg caacagcttc gtcgtgcggg gccccggcgc ctacgacttc
     3241 ctcgaccagc gcgtgcgcgc cgacgacaac ggcgcctgct acgaggaccc gaccgtctcc
     3301 aacctgatga ccagccggat ccgcctcggc cgcgacgagg ccgacagccg caacaacgcc
     3361 gcgtggccga tcccctcgtg cccgccgggc agcgagcggc ccggcgcggg gacgccgaac
     3421 ccctgcttca tcgacacccc gcgcccggag agcctgacct ccgacgcgcc cgagggcctg
     3481 ttccactact tcgactacgg ctacggcctc gaagacagcc ccgtcccggc gatccgcttc
     3541 agtaacccga tgatgagcct ggtcctcgac ctgacctcgc tccagggcct cgtcgacccg
     3601 gtgcccggca ccgaggacga tgtctgggac ccgaagttcc gcgcgtttcg ccgggcccgc
     3661 atcccccgca gcttcaacga gtcgttctcg acggtccccg gctacgcccc cttcgacatc
     3721 ggcgtcatca cctcgagcat cgccctggtc ggcccgacgc ggatcattaa cgcgccggag
     3781 ccgggcatcg tgttcatcgt cgacaccgcc ggcggcaccg gcacaggcgg cgtccgcggc
     3841 caggtcgtgc gcgtgggcct cgtcggcgga cagcagtccc ccgacgagaa gtt
//
LOCUS       DASWNJ010000397         2337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_113102, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000397 DASWNJ010000000
VERSION     DASWNJ010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113102"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VGB85_06820"
     CDS             <1..348
                     /locus_tag="VGB85_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133773.1"
                     /translation="SPPLVRRLCCAALLACALAGALGGCLTGCLTTLGPNITREQHHV
                     RMTPQATRGTCMACHESEAQMTKRMKGMSANEMAEHMRWMTTVVRPPLVQDWMLRERR
                     DCVACHSVKEPRA"
     gene            345..1241
                     /locus_tag="VGB85_06825"
     CDS             345..1241
                     /locus_tag="VGB85_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133774.1"
                     /translation="MSSSRLSHHFSRRLLPALALLAGLSFGTAAWAHHVPGHGASEGL
                     RTINSLNGSGGRAYSRVLLLNEFVYTSFGLNPGLTDSLSVLGEWAPKPSFSVGVQAPL
                     LMFRGADTKGETRVGYGDTRLQLRFTPHAAKLIHRVLTFGVNLSFPTRTVRFTVDPGP
                     VWGATPYIVFTRTYSTVFWQVLGLATVDARRAGTALDLAVSGQVGGRFLKNKLSPGIG
                     ILVDTRVLNFCADPGGGQSFCADSRAGEELRHIGATRATSLATLAYTFVPRASLIASV
                     QAPFTAIRDFSFGWSLGIQGFF"
     gene            1682..>2337
                     /locus_tag="VGB85_06830"
     CDS             1682..>2337
                     /locus_tag="VGB85_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133775.1"
                     /translation="MIRPCSTLLFAALCLASCETEPSEPDAWSVPIKGLPGALLSVWG
                     DASDDIWAVGADTGSGPAVLHYDGTTWKQHSTGATGTLWWVAGQGDDLWMVGDAGLVL
                     HHDRDRDTFDILPAPGPERLYGVMPFAANDVWVVGGNDKDVGVIWQWDGAAWSAPKDL
                     PPGLGDGLAYFKIWGESPADLWVVGDGGGLLHRTGSSWERLDVASKLFTVHGRGDQVL
                     A"
BASE COUNT          322 a          920 c          737 g          358 t
ORIGIN      
        1 tccccgccgc tggtccggcg cctgtgctgc gccgcgctcc tcgcgtgcgc gctcgccggg
       61 gccctcggcg gctgcctgac cggctgcctg accaccctcg gccccaacat cacccgcgag
      121 cagcaccacg tgcgcatgac cccgcaggcg acccgcggca cctgcatggc ctgccacgag
      181 agcgaggcgc agatgacgaa gcgcatgaag ggcatgtcgg cgaacgagat ggccgagcac
      241 atgcgctgga tgacgaccgt cgtccgcccg ccgctggtcc aggactggat gctccgcgag
      301 cgccgcgact gcgtcgcctg ccacagcgtc aaggagccgc gcgcgtgagc tccagccgtc
      361 tctcgcacca tttttcgcgc cgcctgttgc ccgcgctggc gctgctcgcc ggcctgtcct
      421 tcgggacagc cgcctgggcg caccacgtcc ccggccacgg cgcctccgag ggcctgcgca
      481 cgatcaacag cctcaacggc tcgggcgggc gcgcctactc gcgcgtgctc ctgctcaacg
      541 agttcgtcta caccagcttc ggcctcaacc ccggcctgac cgactcgctg tccgtgctcg
      601 gcgagtgggc ccccaaaccc tccttctcgg tcggcgtcca ggccccatta ttgatgttcc
      661 gcggcgccga caccaaaggc gagaccaggg tcggctacgg cgacacccgc ctgcagctgc
      721 gcttcacccc gcacgccgcc aagctgatcc accgcgtgct caccttcggc gtcaacctca
      781 gcttcccgac ccgcaccgtc cggttcaccg tcgaccccgg cccggtgtgg ggcgcgacgc
      841 cctacatcgt gttcacgcgt acctactcga ccgtgttctg gcaggtgctc ggcctcgcca
      901 ccgtcgacgc ccgccgggcc ggcaccgccc tcgacctcgc cgtcagcggc caggtcggcg
      961 gccgctttct caaaaacaag ctcagccccg gtatcggcat cctcgtcgac acccgcgtgc
     1021 tcaacttctg cgccgacccg ggcggcggcc agagcttttg cgccgactcc cgcgccggcg
     1081 aggagctgcg ccacatcggg gccacccgcg cgaccagcct cgccaccctc gcctacacct
     1141 tcgtcccgcg cgcgagcctg atcgcctcgg tgcaggcgcc cttcaccgcg atccgcgact
     1201 ttagcttcgg ctggtcgctc ggcatccagg gcttcttcta ggctggccgc cgcgacgcga
     1261 gcggcgacct cgtcgggcac ggtcaccctg gttgcgacgt gtaatgttct tcgcacgcga
     1321 acgcgagtcg aggcctgcga cgaggcgata tcttttcaga gccacgatcg acgaggtctc
     1381 gcgaacgcgg cgatcgatcg cctgacctcc ggctcgatga tccgtcaacc gacagcgccc
     1441 tggctgcaca cggaggttca tgcgcgaccg cgcacccacg cgcgttcgga tcccacgaca
     1501 gcccgaccat gacgctggtg tcagggtccg gacgccccac gctccccgag cccgcagaac
     1561 gccgctccgc ggccatctcg ccgctggcac cggcgatgca agggggcgcc ccgcgagacc
     1621 ggacggcgag tcacatgacg ccccctgtcc cgcgcagatc ctccccgcta tcgtgccgcc
     1681 catgatccgc ccgtgttcca ctctgttgtt cgccgcgctg tgtctcgcct cctgtgagac
     1741 cgagccgagc gagcccgacg cgtggagcgt cccgatcaag ggcctgcccg gcgcgctgtt
     1801 gtcggtgtgg ggcgacgcca gcgacgacat ctgggccgtc ggcgccgaca ctggctccgg
     1861 tcccgcggtc ctgcattacg acggtaccac ctggaagcag cacagcaccg gcgcgaccgg
     1921 gacgctgtgg tgggtcgccg gtcagggcga cgacctgtgg atggtcggcg acgccggcct
     1981 cgtcctgcat cacgaccgcg accgcgacac cttcgacatc ctgcccgccc ccggccccga
     2041 gcgcctgtac ggcgtcatgc ccttcgccgc gaacgacgtc tgggtcgtcg gcggcaacga
     2101 caaggatgtc ggcgtgatct ggcagtggga cggcgcggcc tggagcgccc cgaaagatct
     2161 gcctccgggc ctcggcgacg gcctcgccta ttttaaaatc tggggcgagt cgcccgcgga
     2221 cctgtgggtc gtcggcgacg ggggcggcct cctgcaccgc accggctcca gctgggagcg
     2281 cctcgacgtc gccagcaaac ttttcaccgt gcacggtcgc ggcgaccagg tgctcgc
//
LOCUS       DASWNJ010000398         1690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_394517, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000398 DASWNJ010000000
VERSION     DASWNJ010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394517"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..>1690
                     /locus_tag="VGB85_06835"
     CDS             17..>1690
                     /locus_tag="VGB85_06835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HEY0133776.1"
                     /translation="MSERDIDSVVGVLAVLKAGAAYVPLDPGYPQQRLACIVEDAAPR
                     WILASAAGAARLPVHGAEILPLDADSPTYADSSEARPEPFVRADDLAYVLFTSGSTGR
                     PKGVAMGHGALRNLVEWHVAHPRLGRSARTLQFASLNFDVSAQELLTTWATGGTLVLV
                     GEDTRRDVLALLGVLEERGVERVYLPFVALQHLAEAASSLGRAPAGLLDVITAGEQLT
                     ITPAIRWLFGRLPACVLHNHYGPTESHVVTAHSLAGPPEAWPTLPPIGRPIDHTRIYL
                     LDAGLEPVPAGVAGELYIAGAALARGYLGRPELTAERFVADPFVAGGTMYRTGDRARR
                     GESGEIEYLGRVDRQVKLRGVRVELGEVEAVLAGHSTVRAAVAAVREDVAGDRRLVAY
                     VVTRDAVPADAAALRAHLRAHLPEAMIPAAFVTLGRLPLLPSGKLDRDALPAPESEAE
                     VRRGEAPTSAAERALAAIWAEVLDIGAERIGLDDPFFDLGGHSLLLARVRAAIAARLG
                     VELRIVDLFRCPTIRSLAVLLRGSEPAPAPAQTQGPRSEGSAIAIVGMSG"
BASE COUNT          190 a          573 c          679 g          248 t
ORIGIN      
        1 cgggcgggtg ggcatcgtgt cggagcgcga catcgattcg gtggtcggcg tgctcgcggt
       61 gctcaaggcc ggcgcggcgt acgtccctct cgaccccggg tacccgcagc agcggctcgc
      121 gtgcatcgtg gaggacgcgg cgccgcggtg gatcctcgcc agcgccgcgg gagccgcgag
      181 gttgccggtg cacggcgcgg agatcctgcc gctggacgcg gattcgccga cgtacgccga
      241 ttccagcgag gcgcggccgg agccgttcgt tcgcgcggac gacctcgcgt atgtgctgtt
      301 cacctcgggc tcgaccggcc gacccaaggg cgtggcgatg ggtcacgggg cgctgcgcaa
      361 cctggtcgag tggcacgtcg cgcacccgcg gctcggccgg tcggcgcgca cgctgcagtt
      421 cgcctcgctc aacttcgacg tctcggcgca ggagctcctg accacgtggg cgaccggcgg
      481 cacgctggtg ctggtggggg aggacacgcg gcgcgacgtt ctggcgctgc tgggcgtgct
      541 cgaggagcgg ggggtcgagc gggtgtatct gccgttcgtc gcgctgcagc acctggccga
      601 ggcggcgtcg tcgctcggcc gggcgccggc gggtctgctc gacgtgatca cggcgggcga
      661 gcagctgacg atcacgccgg cgatccgctg gctgttcggg cggctccccg cgtgcgtgct
      721 gcataatcat tacggtccga cggagtctca cgtggtcacc gcgcactcgc tcgcgggtcc
      781 gccggaggcc tggccgacgc tgccgccgat cggtcggccg atcgatcata cgcggatcta
      841 tctgctcgac gcgggcctcg aaccggtgcc ggccggggtc gcgggcgagc tgtacatcgc
      901 cggcgcggcg ctggcccgcg ggtacctcgg ccggccggag ctgaccgcgg agcgcttcgt
      961 cgccgacccg ttcgtggccg gcgggacgat gtatcgcaca ggcgatcggg cccgccgcgg
     1021 cgagtcgggt gagatcgagt acctcggccg ggtcgaccgc caggtcaagc tgcgcggcgt
     1081 gagggtcgag ctcggcgagg tcgaggcggt gctggccggt cactcgacgg tgcgcgcggc
     1141 ggtggccgcg gtgcgcgagg acgtcgcggg tgaccgccgg ctcgtcgcgt atgtggtgac
     1201 acgcgacgca gtaccggccg acgccgcggc gctgcgggcc cacctgcgcg cgcacttgcc
     1261 ggaggcgatg atcccggcgg cgttcgtgac gctcggcagg ctgccgctgt tgccgagcgg
     1321 caagctcgac cgcgacgcac tgccggcgcc ggagagcgag gcggaggtca gacgcggcga
     1381 ggcgccgacg tcggccgccg agcgggcgct ggcggcgatc tgggccgagg tgctcgatat
     1441 cggggcggag cggatcgggc tcgacgatcc gttcttcgac ctcggcggtc actcgctgct
     1501 cctggcccgc gttcgcgcgg cgatcgcagc gcgactcggg gtcgagctgc ggatcgtcga
     1561 tctgttccgc tgcccgacga tccgctcact ggccgtgctg ctgcgcggga gcgagccagc
     1621 gcctgcgcca gcgcagacgc aggggccgcg cagcgagggc tcggcgatcg cgatcgtcgg
     1681 catgtcgggt
//
LOCUS       DASWNJ010000399         3553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_394523, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000399 DASWNJ010000000
VERSION     DASWNJ010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394523"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VGB85_06840"
     CDS             complement(<1..275)
                     /locus_tag="VGB85_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133777.1"
                     /translation="MKIDLKQFLALAAVMSQGLNAGCTIITDSGDDDGSATNNTAPLT
                     GSGTDTGSSGDATDTDAPTSTSGDDGTGGAPTTGATEATTEATTGATS"
     gene            794..2968
                     /locus_tag="VGB85_06845"
     CDS             794..2968
                     /locus_tag="VGB85_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0133778.1"
                     /translation="MTTGSTGMVACDIACPQHQHCEIVDAMASCVGDDCADLDCGPTE
                     ICVTTGDGAFCQDIACSADLDCPGEQHCDGTVCVDDVCTPGATNCIDDDLHACAANGS
                     AVALKYTCGSDSYFTSACVDPGGGDAHCSCEDDWDCPAFTACAVGRCVGTGNEPTCTL
                     AAVSLAEASPVKEFAWGGTGQNAKDAVGAPFPSSSQVSSQPAVINLDDDNGDGRVDAR
                     DIPEIVFLSYCGADVSANGIVRAVHGGGPGKGKDFFATCGDTVWHEGDDPNLVCACAS
                     ATANSTASIAAGDLDGDSVPEIVVPTETAGLVVLSNTGEPITATLGNQWNAYAGPAPA
                     IANLDNAGPAEIVVGRHVFTVANDGASLVFVDHFSGALMNGSQGLGPIPCVANIAGDS
                     RPEIIAGTTVYRLPVPPPGVMKRADCQVGAIDPFCLGQLTVVWDGQTVNGAGAVPNAQ
                     RDGFCAVADVLGGDETAAPDPANPLDGKAEVVLIAEGTLLVFNGETGALRRSINLGAG
                     IDGGAPVIGDLDGDGFPEIGSALALRFIVHDLQNPSASCPAWPNAFVDAIPGLQGNPA
                     RAPGGACTMDAECSDGAVCDPIRGACVCLHNGWQRVTEDDSSRVTAATVFDFNADGAA
                     EVLYNDECSLRMYGGGMGDLLFKQPSPSRTRIEAPVVADVDDDGDAEIVAPSNNDAPS
                     CSQGVDFPNGIAVHGDAGDVWGAARRVWNEHAYHITNVIRVA"
     gene            3095..>3553
                     /locus_tag="VGB85_06850"
     CDS             3095..>3553
                     /locus_tag="VGB85_06850"
                     /inference="COORDINATES: protein motif:HMM:NF026010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133779.1"
                     /translation="MRELKPREKMYEVTCAAMPGFVVRVLPTGKKVFLVRRQIDGQWR
                     REKIGLMGPSLGVDEARRRAVLMMGVGERDEPTVQPAAGRNAPRSTRRVEHVKQVESG
                     RLTVRDLADRFVREYVDVYLKPGTAINYRKHIADHILPALGDRDFESVTRN"
BASE COUNT          570 a         1187 c         1220 g          576 t
ORIGIN      
        1 gaggtcgcgc cggtggtcgc ctcggtggtc gcctcggtcg cgccggtggt cggcgcgccg
       61 ccagtgccgt cgtcgcccga ggtactggtc ggcgcgtcgg tgtcggttgc atcaccggag
      121 gatcccgtgt ccgtgccgga gccggtcaac ggggccgtgt tgttggtggc ggaaccgtcg
      181 tcgtcgccgc tatcggtgat gatcgtacag ccggcgttga ggccctgact catcaccgcg
      241 gcgagcgcca ggaactgctt cagatcgatc ttcatggtcg cttaaaaaga gtgccaggaa
      301 gcgaggggca ggggcgcgcg tcgccctcga attttcgcgg ttctcaaatg taggcgccgc
      361 gccgtcgtcg cggtgtcgcg aatgcgacga gcaacaggag cgtggagatt caccaccgaa
      421 catccgtggt ctcacggccg ctccggtact cggcctgccg acacgcttcc cgcatgaaag
      481 cactcgagcg cgatttacaa ccccgcctgg atccggcaag ctgccgagat tggtcgggag
      541 gtggtcgatg agacgcggcg ggtggacgac cctggtgctc ggtgtgatgt gccttccggt
      601 gctggcggcg ggctgcgggg acgatgttgc ggcgagtacg agcacaacgg gctcggatac
      661 gaccggaccg ggcagcacga gccgagctcc gaccagcgaa gggctcacca ccggcgggcc
      721 gacggcgccg accaccagcg atgcgagcga ttcgtccggc ggatccaccg atggcgtcct
      781 cagcaccgga ggcatgacga ccggctcgac gggcatggtc gcgtgcgaca tcgcgtgccc
      841 gcagcatcaa cactgcgaga tcgtcgatgc aatggcgtcc tgcgtcggag acgactgcgc
      901 cgacctcgac tgtgggccca ccgagatctg tgtcacgacg ggtgacggtg cgttctgtca
      961 ggacatcgcc tgcagcgccg acctcgattg cccgggcgag cagcactgcg atggtaccgt
     1021 ctgcgtcgac gatgtctgca cgccgggcgc gacgaactgc atcgacgacg atcttcatgc
     1081 ctgtgccgcg aacggcagcg cggtcgcatt gaagtacacc tgcggcagtg actcgtactt
     1141 caccagcgcc tgcgtcgatc ccggtggtgg tgacgcgcat tgcagctgcg aggacgactg
     1201 ggactgtccg gccttcaccg cctgcgcggt cgggcggtgt gtcggtaccg gcaacgagcc
     1261 gacctgcacg ctcgccgcgg tgtcgctggc agaggcctcg ccggtcaagg agttcgcctg
     1321 gggtggtacc ggccagaacg ccaaagacgc tgtcggcgcg ccgtttccga gctcctcgca
     1381 ggtctcctcg cagccggccg tcatcaacct cgacgacgac aacggcgacg gccgcgtcga
     1441 tgcgcgcgac atcccggaga tcgtgttcct cagctactgc ggagccgacg tgagcgcgaa
     1501 cgggatcgtc cgcgcggtcc acggcggcgg gcccggcaag ggcaaggact tctttgccac
     1561 ctgcggtgac accgtctggc acgagggtga cgatccgaac ctggtatgcg cctgtgcctc
     1621 ggcgaccgcc aactccaccg cctcgatcgc tgccggtgac ctggacggcg acagcgtgcc
     1681 cgagatcgtc gtcccgacgg agaccgccgg gctcgtcgtg ctgtccaaca ccggcgagcc
     1741 gatcaccgcg acgctcggta atcagtggaa tgcttacgcg ggcccggcac cggcgatcgc
     1801 aaacctcgac aacgccggtc ccgcggagat cgtcgtcggc cgccacgtct tcacggtcgc
     1861 aaacgacggc gccagtctcg tgttcgtcga ccatttcagc ggcgccctca tgaacggctc
     1921 gcagggtctg ggcccgatcc cctgcgtggc caacatcgcc ggcgactcac gaccggagat
     1981 catcgccggg accacggtct atcggctgcc ggtgccgccg cccggggtga tgaagcgcgc
     2041 cgactgtcag gtcggcgcca ttgacccgtt ctgcctcggc cagctgaccg tcgtctggga
     2101 cggtcagacg gtgaacggcg cgggggctgt cccgaacgcc cagcgcgacg gcttttgcgc
     2161 cgtcgctgat gtgctcggcg gcgacgagac cgccgcgccc gaccccgcca atccgcttga
     2221 cggcaaggcc gaggtcgtat tgatcgccga gggcacgctg ctggtcttca acggtgaaac
     2281 cggcgccctg cgccgctcca tcaacctcgg cgccggcatc gacggcggcg caccggtgat
     2341 cggcgacctc gatggcgacg gcttccccga gatcggcagc gccctcgcgc tacgcttcat
     2401 cgtccacgac ctgcaaaacc cgagcgcgag ctgcccggcg tggccgaacg ccttcgtcga
     2461 cgcgataccc ggtctgcagg gcaaccccgc ccgcgctccc gggggcgcct gcacaatgga
     2521 cgccgagtgc tcggacggcg ccgtctgcga tccgatccgc ggcgcgtgcg tatgtctgca
     2581 caacggctgg cagcgcgtga ccgaggacga ctcgagccgg gtcaccgccg cgaccgtctt
     2641 cgatttcaac gccgacggcg ccgccgaggt cctctacaac gacgagtgct cgcttcgcat
     2701 gtatggcggt gggatgggcg acctgctgtt caagcagccc tcgcccagcc gcacgcgcat
     2761 cgaggcgccg gtcgtcgccg acgtcgacga cgacggcgac gccgagatcg tcgccccgtc
     2821 gaacaatgac gccccatcgt gctcgcaggg cgtcgacttc ccgaacggta tcgccgtcca
     2881 tggtgatgcc ggcgacgtct ggggcgcggc gcgcagggtg tggaacgaac atgcctacca
     2941 catcaccaat gtgataaggg tggcttgaat acgccatgtg cggccttata ctagagaatg
     3001 tgtactttcg gacaggctct tttcggccct tgcctggctc gaatttgtcg cgataactgg
     3061 ggtggagggt ttggacatgc tgactaccgc aacggtgcgc gagctgaagc cgcgcgagaa
     3121 gatgtatgag gtgacgtgcg cggcgatgcc cgggttcgtc gtacgggtgc tgccgaccgg
     3181 caagaaggtg ttcctggttc gccgccagat cgacgggcag tggcggcgcg agaagatcgg
     3241 gctcatgggc ccgtcgctcg gtgtcgacga ggcgcgccgt cgtgcggtgc tgatgatggg
     3301 cgtcggtgag cgcgacgagc cgaccgtcca gccggccgcg ggccgcaacg cgccgagatc
     3361 gacgcgccga gtcgagcatg tgaaacaggt ggaatcaggc cgactgacag ttcgcgacct
     3421 cgccgatcgc ttcgtccgcg agtacgtgga cgtctatctg aagcccggca cggcgatcaa
     3481 ctaccgcaag cacatcgccg atcacatcct gccggcgctc ggcgatcgtg acttcgagag
     3541 cgtgacccgc aac
//
LOCUS       DASWNJ010000400         6082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_431395, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000400 DASWNJ010000000
VERSION     DASWNJ010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6082
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431395"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VGB85_06855"
     CDS             complement(<1..438)
                     /locus_tag="VGB85_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133780.1"
                     /translation="MSLGTWLMAQEPVQAGPAEPKLVRPLRPELAPRRRGLVFAFMPA
                     LTFGLSPLPSLDHAFYFGGRLRGPWALGYQFTLSSGGAERYVLGLMTHRHHITGMRGF
                     GRDDRGLISVGGGAAFLFAVPVVEVETRVAWRFGKRRRGLLGGV"
     gene            complement(459..1277)
                     /locus_tag="VGB85_06860"
     CDS             complement(459..1277)
                     /locus_tag="VGB85_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133781.1"
                     /translation="MRVAAFVCGALVAGAAARAAATSCASGWFSLDSEGTVSGFEPAP
                     VAVDASPWAYEYCGAAVLKSCVLQAGDEVIAVEVRTVGEDACADPQSRLADNLRPGYI
                     RYFVPARPLIRGEEHALVCDGQDTGMTVDVLAKSDSAPPATLDIGAANVRQSDGGCCG
                     GEDYLEVEFESSSDDFLSDGGYVEIAYASGHVLVLGHAWDSLELPPTEGTISFTPVAA
                     DGERGETVRLEPDDVHWDAVYLPCGVDRRGGSLGLWLLAPLLWVRSRSRRPGRR"
     gene            complement(1323..2093)
                     /locus_tag="VGB85_06865"
     CDS             complement(1323..2093)
                     /locus_tag="VGB85_06865"
                     /inference="COORDINATES: protein motif:HMM:NF041144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030248 - cellulose binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="expansin EXLX1 family cellulose-binding protein"
                     /protein_id="HEY0133782.1"
                     /translation="MKRRTGSGALWLGVTLACGGNGDATTGEDPDGSDTSDSGQLTGE
                     TTAEPTSAGEVCEAGQHTGEATYYGADGSGNCSFDAGPDILIAAMNDADYANAAACGA
                     CVEITGPDATIVVRIVDRCPGCPQGDIDLGEVAFTKIAAKELGRVPISWRYVSCPVEG
                     PLVYRFKEGSSEYWTAVQVRNHRNAITSFEYREGGGAWQPVARVEYNYFIDEGGMGPG
                     PIDFRVTDVAGNVVEDYGLMVVDGGVASGAGQFPGCMP"
     gene            2299..2670
                     /locus_tag="VGB85_06870"
     CDS             2299..2670
                     /locus_tag="VGB85_06870"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0133783.1"
                     /translation="MAEGYPRLMAQEVALITSELAWNIVRHGGGGTIELAIIVDPQYG
                     PGIEIHACDSAPPIADLALAQRDGHTAIGPIPAEVMLTRRGIGSGLGAVARLGDRLLQ
                     VPRGTGKCLVVTRFLTRPRPA"
     gene            complement(2636..3511)
                     /locus_tag="VGB85_06875"
     CDS             complement(2636..3511)
                     /locus_tag="VGB85_06875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0133784.1"
                     /translation="MADFARFFQYSLDLLATLDAEGRFVDLNPMWTRVMGYSPEELRG
                     RPFAELIHPEDLAPAVTLMHGEAKTSELTSVENRYRSKSGEYRWLSWSTAPPGPDGLF
                     YTSARDITAYKETVAEREQALTLSTQFEADYRRVEDELRRRLEAQQDAIHSMSTPIIQ
                     VWDDIVTMPVVGLVDSVRAADMKNSLLETVSRTSAKFAIVDLTGVDTVDTATADHLLK
                     VMRAVGLLGARCVITGIQPSVAQIIVALGLDMQGVITLRSLREGLKYCMRTMGLRVVR
                     EEPKVTLDVDASGSA"
     gene            complement(3566..4675)
                     /locus_tag="VGB85_06880"
     CDS             complement(3566..4675)
                     /locus_tag="VGB85_06880"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0133785.1"
                     /translation="MSDRQPNIVVEGLDFRWDLERGTFSISGVPCTSFFRDSSLASLL
                     MGFLSMVGPQRFSLALRSEGQRGIDEDWQIIGAEPTFEAGFAKLALFAYTCGWGRWEC
                     VDLDREAKQAVFRVHDSWEGLAQRANGVNWGPGLVAGKFTAFGARLFGTNCWCRQTMF
                     IADGDPYDEMVVEASDRDIAQELAELAQDDQATSSDLQRALTDLRSTAEAHARALGER
                     DRSVADMQEKLDIIARQQAAIQALSTPIIQVWDGVLAVPVVGAVDGERTSRMMEKLLE
                     TIIHTKSRYAIIDVTGVETVDTHTADNFVRLIRGVQLLGAQGIISGIGPLVAQTIVDL
                     GVDLAGIPTFANLKEALHACIGRTVVRHRTGGASQ"
     gene            complement(4687..5115)
                     /locus_tag="VGB85_06885"
     CDS             complement(4687..5115)
                     /locus_tag="VGB85_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0133786.1"
                     /translation="MSNYENRIPVIQLWQRIVVPLQGDVTDETAAVLVDDVLRTIRDT
                     SAAGLVIDVTGMWTMDSHLCHVIANLAAAARLMGTPTVICGMSAPIAMTLQTMGADFR
                     LAQTALTLEDALERLGVTATVTEIDPDDPDGQAAEAYELA"
     gene            complement(5112..5690)
                     /locus_tag="VGB85_06890"
     CDS             complement(5112..5690)
                     /locus_tag="VGB85_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133787.1"
                     /translation="MPVSYVMAHRTIPKQGEFANGDAVVLRVEGEVALVAAIDALGHG
                     ELAEYAAKKAIAFLEAWSLSDDLRDGMQGLHEALRGSRGAAASVCLLRGQDLTFCGVG
                     NVEVRCFGTKAPILLSPGVLGARVQQFRMCRATLADGSRLVLYSDGISHRAPFEALHQ
                     LDPEGLVEELIKNHRKTSDDATVVACDLRAKP"
     gene            complement(5690..>6082)
                     /locus_tag="VGB85_06895"
     CDS             complement(5690..>6082)
                     /locus_tag="VGB85_06895"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0133788.1"
                     /translation="KVDAIELEVRVESDIARARLAARDLCQALGTSPLVVQKLATIVS
                     ELARNIISYAGTGNLAIRQCSGGRRCVHVEAKDQGKGIANLEEILAGKYKSRSGLGLG
                     ILGTKRLADQFRIESSSAGTRVEVEVGY"
BASE COUNT          939 a         2175 c         2045 g          923 t
ORIGIN      
        1 gacgccgccg agcagaccgc ggcggcgctt gccgaagcgc cacgccacgc gggtctcgac
       61 ctcgaccacc gggacggcga acaaaaacgc cgccccgcca ccgaccgaga tcaggccgcg
      121 atcatcacgg ccgaagccgc gcatgccggt gatatggtgg cgatgcgtca tcagccccaa
      181 tacgtagcgc tcggccccgc cgctcgacaa cgtgaactga tagccgagcg cccacggccc
      241 gcgcaggcgc ccgccgaagt agaacgcgtg gtcgagcgag ggcaagggcg acagcccgaa
      301 ggtcagcgcc ggcatgaacg cgaacaccag accacgccgc cgcggcgcga gctccggtcg
      361 caggggccgg acgagcttcg gttcggccgg cccggcctgc accggctcct gcgccatgag
      421 ccatgtcccg agggacatgc ccacaaagac agccacgatc atcgacgccc cggccggcgg
      481 cttcggctgc ggacccacag cagcggcgcg agcagccaca ggccgagcga gccgccgcgc
      541 ctgtcgaccc cgcaggggag gtacacggcg tcccagtgga catcgtcagg ctcgaggcgg
      601 accgtctcgc cacgctcccc gtccgctgcg accggcgtga acgagatcgt cccctcggtc
      661 ggtggcagct ccagcgagtc ccacgcgtgc ccgaggacca gtacatgccc cgacgcatac
      721 gcgatctcca cgtacccacc gtccgacagg aaatcatcgg agctactctc gaactcgacc
      781 tcgaggtaat cctcgccgcc gcagcagccc ccgtcgctct gacgcacgtt ggcggcgccg
      841 atgtcgagcg tcgccggcgg cgccgagtcg ctcttggcga gcacatcgac ggtcatgccg
      901 gtgtcctggc cgtcgcagac cagcgcgtgc tcctcgccgc ggatgagcgg ccgcgcgggc
      961 acgaagtagc gtatgtaacc cggccgcaga ttatcggcga gacgcgactg cgggtccgca
     1021 caggcatctt caccgaccgt acgcacctcg acagcgatca cttcatcgcc agcttgcagc
     1081 acgcacgact tcaggacagc cgcgccgcaa tattcataag cccacggcga cgcgtccacg
     1141 gccaccggcg ccggctcgaa ccccgacacg gtcccctccg agtccagcga gaaccacccc
     1201 gacgcgcacg aggtcgccgc ggcgcgagcc gccgcgccgg cgacgagcgc cccacacacg
     1261 aaggccgcca cgcgcatctc gccatcgtac ccgaagcccc accacgcgtc gaggcacctc
     1321 tcctaaggca tacagcccgg aaactgcccg gctcccgagg ccaccccgcc gtcgacgacc
     1381 atcaggccgt aatcctcgac cacgttgccc gcgacatcgg tgacacgaaa gtcgatcggt
     1441 cccggcccca tgccgccctc gtcgataaaa tagttgtact cgacgcgcgc gaccggctgc
     1501 cacgcgccgc cgccttcgcg gtactcgaag ctggtgatcg cgttacgatg gttgcggacc
     1561 tgcactgccg tccagtactc gctgctgccc tccttgaagc ggtagaccag cgggccctcg
     1621 accgggcacg acacgtagcg ccaggagatc ggcacgcgcc cgagctcctt cgcggcgatc
     1681 ttggtgaacg ccacctcgcc gaggtcgatg tcgccttgcg ggcagcccgg gcagcggtcg
     1741 acgatccgca ccacgatcgt cgcgtcgggt ccggtgatct cgacacaggc gccgcaggcc
     1801 gccgcgttcg cgtagtcggc gtcgttcatc gccgcgatca ggatgtccgg ccccgcgtcg
     1861 aagctgcagt tgccgctgcc gtcggccccg taataagtcg cctcaccggt gtgctggccg
     1921 gcctcgcaca cctcgcccgc ggaggtcggt tccgcagttg tttcgccggt caactgtccg
     1981 ctgtccgacg tatccgaacc gtccggatcc tccccggtcg tcgcatcgcc gttgcctccg
     2041 caggccagcg taacgcccag ccacagcgct cccgagcccg ttcgccgctt catgccagca
     2101 aggacaccgc aggcccgcgg cgctgtcaaa tgtccggcgg ttcgggcgcg agcgtggggg
     2161 cggcgattca ttcctgtcct ttcagacagc cgcacatcag cctggacgtg gtatgcatgg
     2221 tgggtggcgc cgcagcggta cccggtcgcg ctggatgccg acgtatacac ggctcgccag
     2281 gccgcggggc ggatggcaat ggccgagggt tacccgcgcc tgatggctca ggaggtcgcg
     2341 ctgatcacct ccgagctcgc gtggaacatc gtgcgtcacg gcggcggcgg gaccatcgag
     2401 ctggcgatca tcgtcgaccc gcagtacggg cccggcatcg agatccacgc ctgtgacagt
     2461 gcgccgccga tcgccgacct cgcgctggcg cagcgtgacg gtcacacggc gatcggcccg
     2521 atccccgcgg aggtcatgtt gacgcggcgt gggatcggca gcgggctcgg ggcggtcgcg
     2581 cggctcggcg accgtctgct tcaggtgccg cgtggcaccg gcaagtgtct cgtcgtcacg
     2641 cgcttcctga cgcgtccacg tccagcgtga ccttgggctc ctcgcggacg acccgcagcc
     2701 ccatcgtccg catgcagtac ttgaggccct cacgcagcga gcgcagcgtg atgacgccct
     2761 gcatgtcgag cccgagcgcc acgatgatct gcgcgaccga gggctggatg ccggtgatga
     2821 cgcagcgggc gcccagcagg ccgaccgcgc gcatcacctt gagcaggtgg tcggcggtcg
     2881 ccgtgtcgac ggtgtcgacg cccgtcaggt cgacgatcgc gaacttggcg ctcgtgcgcg
     2941 acacggtctc cagcagcgag ttcttcatgt cggccgcgcg cacgctgtcg accaggccga
     3001 cgaccggcat cgtcacgatg tcgtcccaca cctgaatgat cggcgtcgac atcgagtgga
     3061 tcgcgtcctg ctgggcctcg aggcggcggc gcagctcgtc ctcgacgcgg cgatagtcgg
     3121 cctcgaattg cgtcgacagc gtcagcgcct gctcgcgctc ggcgaccgtc tccttgtacg
     3181 cggtgatgtc acgcgcgctg gtatagaaca gcccgtccgg gcccggaggg gccgtgctcc
     3241 acgacagcca gcgatactct ccgctcttgc tgcgatagcg gttctcgacg ctcgtgagct
     3301 cgctggtctt cgcctccccg tgcatcagcg tgaccgccgg cgcgaggtcc tcggggtgga
     3361 tgagctccgc gaacggccga ccgcgcagct cctcgggcga gtagcccatg acgcgcgtcc
     3421 acatcgggtt gaggtcgacg aaccggccct ctgcgtcgag ggtggcgagc aggtcgagcg
     3481 agtactgaaa gaagcgggca aagtcagcca tggcgcgggt cctcggcgat gatagaggcg
     3541 tgggggccgt aatagtagtg gccggtcatt gcgaggcccc tccggtgcga tgccgaacga
     3601 ccgtgcggcc gatgcatgcg tggagggcct ccttgaggtt ggcaaaggta gggatgcctg
     3661 cgaggtcgac cccgaggtcg acgatcgtct gtgcgaccag cgggccgatg ccagagatga
     3721 tgccctgggc cccgagcagc tggacgccgc ggatcagccg cacgaagttg tccgcggtgt
     3781 gcgtgtcgac cgtctcgacg ccggtgacgt cgatgatcgc gtagcgcgac ttcgtgtgga
     3841 tgatcgtctc gagcagcttc tccatcatgc gactggtgcg ctcgccgtcg accgcgccga
     3901 cgaccggcac cgccagcacg ccgtcccaca cctgaatgat cggtgtcgac agcgcctgga
     3961 tcgccgcctg ctggcgcgcg atgatgtcga gcttctcctg catgtccgcg acgctgcggt
     4021 cgcgctcccc gagcgcgcgc gcgtgcgcct ccgcggtgct gcgcaggtcg gtcagcgccc
     4081 gttgcaggtc gctcgaggtc gcctggtcgt cctgcgccag ctcggccagc tcctgcgcga
     4141 tgtcgcggtc cgaggcctcg accaccatct cgtcgtacgg gtcaccgtcg gcgatgaaca
     4201 tcgtctgacg gcaccagcag ttggtcccga acaggcgcgc gccgaaggcc gtgaacttgc
     4261 cggcgacgag gccggggccc cagttgacgc cgttggctcg ctgcgcgagg ccctcccagc
     4321 tgtcgtgcac gcggaacacc gcctgcttgg cctcgcggtc gaggtcgacg cactcccagc
     4381 ggccccagcc acaggtatag gcgaagagcg cgagcttggc gaagccggcc tcgaaggtcg
     4441 gctcggcccc gatgatctgc cagtcctcgt cgatgccgcg ctggccctcg gagcgcagcg
     4501 ccagcgagaa gcgctgcggc ccgaccatgc tcaggaagcc catcaacagg ctggccagcg
     4561 agctgtcgcg gaagaacgac gtgcacggga cgcccgagat gctaaaggtg ccgcgctcga
     4621 ggtcccagcg gaaatcgagg ccttcgacga cgatgtttgg ttgacggtcg ctcatggttc
     4681 gtgggctcag gcgagctcgt aggcttccgc ggcttggcca tcggggtcgt cggggtcgat
     4741 ctccgtcacc gtcgcggtga cgccgaggcg ctcgagcgcg tcctcgaggg tgagggccgt
     4801 ctgggccagg cggaagtcgg cgcccatcgt ctgcagcgtc atcgctatcg gcgcgctcat
     4861 gccacaaatg acggtcgggg tgcccatgag cctcgcggcg gcggcgaggt tggcgatgac
     4921 gtggcacagg tggctgtcca tcgtccacat gccggtgacg tcgatgacga ggccggccgc
     4981 ggacgtgtcg cggatcgtgc ggagcacgtc gtcgaccagc accgcggcgg tctcgtcggt
     5041 gacgtcgccc tgcagcggaa cgacgatacg ctgccagagc tggatgacgg ggatgcggtt
     5101 ctcgtagttg ctcatggctt ggccctcagg tcacacgcga ccacggtcgc gtcgtcgctc
     5161 gtcttgcggt ggttcttgat cagctcctcg accagaccct cggggtcgag ctggtggagc
     5221 gcctcgaagg gcgcgcggtg cgagatgccg tcgctgtaga gcaccagccg gctgccgtcg
     5281 gccagcgtcg cgcggcacat gcgaaactgc tgcacccgcg ccccgagcac cccggggctg
     5341 agcaggatcg gcgccttggt gccgaagcag cggacctcga cgttgccgac gccgcaaaat
     5401 gtcaggtcct gaccccgcag caggcacacg gaggccgcgg cgccccggga cccgcgcagc
     5461 gcctcgtgca ggccctgcat cccgtcgcgc aggtcgtcgc tcagcgacca cgcctcgagg
     5521 aacgcgatcg ccttcttggc cgcgtactcg gcgagctcgc cgtggccgag cgcgtcgatc
     5581 gcagcgacca acgcgacctc gccctcgacg cgcagcacca ccgcgtcgcc gttggcgaac
     5641 tccccctgct tggggatcgt tcgatgcgcc atcacgtaag agaccggcat cagtaaccga
     5701 cctccacttc gacgcgggtg cccgcggagc tcgactcgat gcgaaactga tcggcgaggc
     5761 gcttggtgcc gaggatcccg aggccgaggc cgctgcggct cttgtacttg ccggcgagga
     5821 tctcctcgag gttggcgatg cccttgccct ggtccttggc ctcgacgtgc acgcagcgac
     5881 gcccgccgct gcattgacgg atcgccaggt tgccggtgcc cgcgtagctg atgatgttgc
     5941 gcgccagctc gctgacgatc gtcgccagct tctggaccac caacggcgag gtgccgagcg
     6001 cctggcacag gtcgcgcgcg gccagccgcg cccgcgcgat gtccgactcg acgcgcacct
     6061 cgagctcgat cgcgtcgacc tt
//
LOCUS       DASWNJ010000401         1916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_466750, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000401 DASWNJ010000000
VERSION     DASWNJ010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466750"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="VGB85_06900"
     CDS             <1..1150
                     /locus_tag="VGB85_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133789.1"
                     /translation="APTTGDAATTDATTGSSSSGTTRAVSDTGDASTSTSSTSSTSST
                     GEESSTSTGEEPPVDLCAHQDDPPPAVIRKAINDDPRFIQVYDDNIENLKLAGEQCAG
                     DWTDLIYYMKTIEPSPDVFLVQQISDPAQLEVLVDRLETELPGLFEGFISDAEPWPQM
                     SPCGKEKALQTNAIILRTGRFEVVGDKHVWQSWANKDGECVRNNQARTRNVMVKLHDK
                     IADKHVTVASMHWSTSQGAGDDPACAEANVLEVDEKLHKAGFGGDMVIFGGDFNESDR
                     NDMGDFRAWYKLANGDGGGALNYRDPIYRMCDATDNLKDCLDDNDTGGKRIDMLFAQD
                     QNGCRVRTARAHTITYAEAEAAAKQLSGDSDPDLHYSEHRALRAEFYY"
     gene            1303..>1916
                     /locus_tag="VGB85_06905"
     CDS             1303..>1916
                     /locus_tag="VGB85_06905"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0133790.1"
                     /translation="MNATKPTVVIAGAGPAGMLLAYQLVSNGVPVRVLEQHADFDREF
                     RGDLIGPSVLPTLERLGLLPILVARGQARAGVERCMFVGARRRVTLPMGKELGALVSQ
                     AGLLGLLHELCSRHPHYEMQFGTTALRAIREGERVVAIETRRDGVAGRVDGDVFVACN
                     GRNSRLRKDIGIPLELDVKPDTTLWLRFDFTDAKQALPETLDVH"
BASE COUNT          339 a          645 c          672 g          260 t
ORIGIN      
        1 ggcaccgacg acgggtgacg cggcgaccac cgacgcgacg acgggctcgt cctcgtccgg
       61 aacgaccagg gcggtctccg acacggggga tgcatcgacc agcacgtcga gcacgtcgag
      121 cacgtcgagc acgggcgagg agtcgagcac gagcacgggc gaggagccgc cggtcgacct
      181 gtgcgcgcac caggacgacc cgccgccggc ggtcatacgc aaggcgatca acgacgaccc
      241 gcggttcatc caggtctacg acgacaacat cgagaacctg aagctcgcgg gggagcagtg
      301 cgccggcgac tggaccgatc tgatctatta catgaagacg atcgagccga gccccgacgt
      361 gttcctcgtg cagcagatct cggatcccgc gcagctcgag gtgctggtcg accgcctcga
      421 gaccgagcta ccggggttgt tcgagggctt catctccgac gccgagccgt ggccgcagat
      481 gtcgccgtgc ggcaaggaga aggcgctgca gaccaacgcg atcatcctcc gcacgggccg
      541 cttcgaggtc gtcggcgaca agcacgtgtg gcagtcgtgg gccaacaagg acggcgagtg
      601 cgtgcgcaac aaccaggcgc gcacgcgcaa cgtgatggtc aagctgcacg acaagatcgc
      661 cgacaagcac gtcacggtcg cgtcgatgca ctggtcgacg agccaggggg cgggcgacga
      721 cccggcgtgc gccgaggcga acgtgctcga ggtcgacgag aagctgcaca aggccggctt
      781 cggcggcgat atggtgatct tcggcggcga cttcaacgag tcggaccgca acgacatggg
      841 cgacttccgg gcctggtaca agctcgccaa cggcgacggc ggcggcgcgc tcaactaccg
      901 cgacccgatc tatcgcatgt gcgacgccac cgataatctc aaggattgcc tcgatgataa
      961 tgacaccggc ggcaagcgca tcgacatgtt gttcgcgcag gaccaaaacg gctgccgcgt
     1021 ccgcaccgcg cgggcgcaca cgatcacgta cgccgaggcc gaggcggccg ccaagcagct
     1081 cagcggcgac agcgacccgg acctccacta ttcggagcac cgcgccctgc gcgcggagtt
     1141 ttactattga gcatagttcc tcgcggcacg cgtcggcagc gaccaggcgc aggccccggc
     1201 gacgatcgcg gccggccgct tctccgcggt gacgctgcgg atgcgccgag ctgcggacgc
     1261 ggaggctcgc gctcgatagc gcgcccgtgg taggacgggc cggtgaacgc aacaaagccg
     1321 acagtcgtca tcgccggggc cgggcccgcg ggcatgctgc tcgcgtacca gctcgtcagc
     1381 aacggcgtgc ccgtgcgtgt gctcgagcaa cacgccgact tcgaccgcga gttccgcggc
     1441 gacctgatcg gtccatccgt gttgccgacc ctcgagcggc tcggcctgct gccgatcctc
     1501 gtcgcgcgcg ggcaggcgcg ggcaggcgtc gagcgctgca tgttcgtcgg cgcccgccgc
     1561 cgcgtcacgc tgccgatggg caaggagctc ggcgcgctgg tgtcccaggc cgggctgctc
     1621 ggcctgctgc acgagctgtg cagccgccat ccgcactacg agatgcagtt tgggaccacc
     1681 gcgctgcgag cgatccgcga gggcgagcgg gtggtggcga tcgagacccg gcgcgacggc
     1741 gtggcggggc gcgtggacgg cgatgtgttc gtcgcgtgca acgggcgcaa cagtcgcctg
     1801 cgcaaagaca tcgggatccc gctcgagctc gacgtcaagc ccgacaccac gctgtggctg
     1861 cgcttcgact ttaccgacgc gaagcaggcg ctgccggaga cgctcgatgt tcatat
//
LOCUS       DASWNJ010000402         4603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_42696, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000402 DASWNJ010000000
VERSION     DASWNJ010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42696"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VGB85_06910"
     CDS             <1..479
                     /locus_tag="VGB85_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0133791.1"
                     /translation="RSPVRIGRDAGNELRLPFSFVSGRHAVLQFDDERAELLDAGSTN
                     GVLREGRRLPSRQAVPIVDTLTVTIGRLELRVRRLTADDSPSAPPIAPEAAAADATGL
                     ARVHGAIRDLRPLYIEMLQARSTFVAARDAALAGIPAEARDLADAMLAREFPDPAK"
     gene            488..1456
                     /locus_tag="VGB85_06915"
     CDS             488..1456
                     /locus_tag="VGB85_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133792.1"
                     /translation="MRPLAAASLLLLAAGCRPKGPVNLCPNLEVASEAAAEREEGRAL
                     DVRAGLTSLIPTLDSGKMLPPAEIRDCREQRVLVPLNDCEDQPIPAIRLPDRPLDEQD
                     LVITDLEGDGFLVWAQAHHFTDGDALGPVAIASWTKRGIRIAAVGPLRGPAKHVRLRL
                     EPLSDGQVLIVEGDACPPDGSACTRVVRIVPIIDDQFVDKSMRLNEGGCGGPATFPLS
                     ASHETLVDPTKKRRFLLQRNVVIKDGLGIVHEEAIIKDVDPRQPNAEGVEFRRVTKAR
                     ELLLDKDGLLVQKGIWQDMLAEHGSVRPSDHVDPAPAEPKAKKRGR"
     gene            1641..2180
                     /locus_tag="VGB85_06920"
     CDS             1641..2180
                     /locus_tag="VGB85_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133793.1"
                     /translation="MHGSRLHHHALAIVLALSADTSAPPPSRPPAAAASTSDSPPASL
                     TLPPPEQQRSERAARRLRIGGYVTLALGCGLLGTMAAAAAFHRQGIARLDARVAALTP
                     GERLDPFQRLSARQNIDSAQMDRDVAIGTGVSAALSLALATTLFILARRHDRGRSRLA
                     LAPAWLPSGAGLTFQVRLR"
     gene            2198..3304
                     /locus_tag="VGB85_06925"
     CDS             2198..3304
                     /locus_tag="VGB85_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133794.1"
                     /translation="MRSPLPIGPLLPVALLAACFAPTGSGAAVATTSDSTTSGSTDLD
                     PTGPDPSPTTTPTSEATEPTTTTSTSTTGDAESTAGTLDPPAPGCGDGVVDPDTEQCD
                     QGYGSNSDQGECTLHCKNNVCGDQNLHVGVEQCDNGANNNDTLYGACGTDCRWTEHCG
                     DSQVQGPEECDLGPNNGSGLSELGGVGCDPTCRFDAKLCFLTSAVYKADSFLGAELAD
                     DACRAAALDAGFDNAAGFMAWISDDASSPATRFTPSAIPYVLPNGVRIANHSKQLMST
                     GPIAGITRTDKGEDLLKAWVWTGTAPNGTLADPALDCENWTSASFEHQARVGRSGVDE
                     QNLDEWNVWKYEDHWTNLQSFHCAHSYHLYCFEQ"
     gene            3319..4194
                     /locus_tag="VGB85_06930"
     CDS             3319..4194
                     /locus_tag="VGB85_06930"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0133795.1"
                     /translation="MTRPRRMLLSLLALQPACALEHLEAALAEHEDTYDTTNPAGSDT
                     TAPDTTARDSTTTDTTTSSAGSGSDTTGEPGTTASTDTTTTADPGTTTTTGPPPVCGD
                     GLQDPGEECDDGNADPMDGCKDCARDRFIFVTSVEYQGGEIDGLYGADQRCRTQAALA
                     KLPNFATYRAWLSDSKLAAADRLLHHPGRYTLVNGLVVAMDWADLTDGTLENPITVTE
                     FSESPDSVPVWTGTLHNGKSAFGSSFCFDWTDTNSNWDLELGGSGIRTQTDALWSYLA
                     QDGCGSTAALYCLEQ"
     gene            4212..>4603
                     /locus_tag="VGB85_06935"
     CDS             4212..>4603
                     /locus_tag="VGB85_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0133796.1"
                     /translation="MPRIRPIILALPLACGGSRDDTMNPVDDTMNPAGSDTTAPDTTA
                     QDSTTTDTTITSSAGSGSDTTGEPGTTSSTDTTTTADPGSTTTTTTGTPPVCGDGLQD
                     PGEECDDGNADPMDGCKDCARDRFIFVT"
BASE COUNT          760 a         1895 c         1344 g          604 t
ORIGIN      
        1 tccgctctcc ggtgcggatc ggtcgtgacg ctggcaacga gctgcgcctg cctttctcgt
       61 tcgtctcggg ccgccacgcc gttctccagt tcgacgacga gcgcgccgag ctgctcgacg
      121 ccgggtcgac caacggcgtc ctgcgcgagg gccggcgcct gccctcgcgc caggccgtcc
      181 cgatcgtcga caccctgact gtcacgatcg gccgcctcga gctgcgcgtg cgcaggctga
      241 ccgccgacga ctcgccgagc gcccccccga tcgcccccga ggccgccgcc gccgacgcca
      301 ccggcctggc ccgcgtccac ggtgcgatcc gcgacctgcg gccgctctac atcgagatgc
      361 tgcaggcccg cagcaccttc gtcgccgccc gcgacgccgc cctcgccggg atccccgccg
      421 aggcgcgcga cctcgccgat gccatgctgg cgcgcgagtt ccccgatccg gcaaaatgac
      481 cccacgcatg cgccccctcg ccgccgcctc gctcctgctc ctcgccgccg gctgccgtcc
      541 gaagggtccg gtcaacctgt gtcccaacct cgaggtcgcg tccgaggccg ccgccgagcg
      601 ggaagagggc cgcgcgctcg acgtccgcgc cggcctcacc agcctgatcc ccaccctcga
      661 cagcggcaag atgctgccgc ccgccgagat ccgcgactgt cgcgagcagc gcgtgctcgt
      721 cccgctcaac gactgcgagg atcagccgat ccctgcgatc cgcctgcccg atcgtccgct
      781 cgacgagcag gacctcgtga tcaccgacct cgagggtgac ggcttcctcg tgtgggccca
      841 ggcccaccac ttcaccgacg gcgacgccct cggcccggtg gcgatcgcca gctggaccaa
      901 gcgcggcatc cggatcgccg ccgtcggccc cctgcgcggc cccgccaagc acgtgcgcct
      961 gcgcctcgag cccctcagcg acggtcaggt cctcatcgtc gagggcgacg cttgcccgcc
     1021 cgacggcagc gcctgcacgc gcgtcgtccg gatcgtcccg atcatcgacg accagttcgt
     1081 cgacaagtcg atgcgcctca acgagggcgg ctgcggcggc ccggccacct tcccgctcag
     1141 cgcctcgcac gagaccctcg tcgacccgac caagaagcgc cgctttctcc tccagcgcaa
     1201 cgtcgtcatc aaggacggcc tcggcatcgt ccacgaggag gcgatcatca aagacgtcga
     1261 tccgcgccag cccaacgccg agggcgtcga gtttcgccgc gtcaccaagg ccagggagct
     1321 actgctcgac aaggacggcc tgctcgtcca aaaaggcatc tggcaggaca tgctcgccga
     1381 gcacggatcc gtgcgcccca gcgaccacgt cgaccccgcg ccagcggagc ccaaagcaaa
     1441 gaagcgcggc cgctagcgac cgcgacgcct cgccttttga ataacgtgtg taagattcgt
     1501 aatcctcatt actaaaccgt ccttcaaagt aatctccgag gttactttcg cgagaaaacc
     1561 ttgacattca tcgctgccgg ggaccaaggt cgcatcgttc gagcgttgtc gcgcctcctt
     1621 ggcgacgcaa ccacgaatga atgcacgggt cccgattgca ccaccacgcc ctcgccatcg
     1681 tcctcgcgct ctccgccgac acgtcggcgc caccgccgag ccgaccaccg gccgccgcgg
     1741 cgagcacgtc cgacagtccg cccgcctcgc tgaccctgcc gccaccggag cagcagcgta
     1801 gcgagcgtgc ggcgcgtcgg cttcgcatcg gaggctacgt cacgctcgcg ctcgggtgcg
     1861 gtctgctcgg gacgatggcc gcggccgccg cgttccacag gcaaggcatc gcccgcctcg
     1921 acgctcgcgt cgccgcgctg acgcccggcg agcgcctcga cccgttccag cgcctgagcg
     1981 cgcggcagaa catcgacagc gcccagatgg accgcgacgt ggccatcggg acaggtgtct
     2041 ccgccgcgct gtcgctcgcg ctcgcgacca cgctgttcat cctggcccgc cggcacgacc
     2101 gcggccgctc ccgcctcgcc ctcgcccccg cctggctgcc atccggcgcc gggctcacct
     2161 tccaggtgcg cctgcgttga tctcacgaaa gccgcccatg cgctctccac tccccatcgg
     2221 acctctcctc cccgtcgcgc tcctcgccgc ctgcttcgcg ccgaccggct ccggcgccgc
     2281 tgtggcgacc acttcggact cgaccacttc gggctcgacc gacctggacc cgaccgggcc
     2341 cgacccgagc ccgacgacca cgccgaccag cgaggcgacg gagccgacca ccacgacctc
     2401 gacgtcgacc actggcgacg ccgagtccac cgccggcacc ctcgacccac ccgcacccgg
     2461 ttgcggcgac ggcgtcgtcg accccgacac cgagcaatgt gaccagggct acggctcgaa
     2521 cagtgatcag ggggagtgca cccttcactg caaaaacaac gtatgtggcg accaaaacct
     2581 ccacgtcggg gttgagcagt gcgataatgg tgcaaataat aacgacacgc tctatggggc
     2641 atgcggcacc gactgccggt ggaccgagca ctgcggagat tcgcaggtcc aggggcccga
     2701 ggagtgcgac ctgggcccca acaacggctc gggcctcagc gagctcggcg gcgtcggctg
     2761 cgatcccacc tgccgcttcg acgccaagct atgcttcttg acgagcgctg tctacaaggc
     2821 cgacagcttc ctcggcgccg agctggccga tgacgcgtgc cgtgctgccg cgctcgacgc
     2881 cggttttgat aacgccgccg gcttcatggc ctggatcagc gacgacgcct cgagccccgc
     2941 gacccgcttc accccctcgg cgatccccta cgtccttccc aacggcgtcc gcatcgccaa
     3001 ccacagcaag cagctgatga gcaccggccc gatcgccggc atcacccgca ccgacaaggg
     3061 cgaggacctg ctcaaggcct gggtctggac cggcaccgcc ccgaacggga ccctcgccga
     3121 cccggccctc gactgcgaga attggaccag cgccagcttc gagcaccagg cccgcgtcgg
     3181 tcgctccggc gtcgacgagc agaacctcga cgaatggaat gtgtggaagt acgaggatca
     3241 ctggacaaat ttacagtcct tccactgcgc tcacagctat cacctctact gttttgaaca
     3301 gtgatccgag ggccctccat gacccgccca cgccgcatgc tcctgtccct cctcgccctc
     3361 cagcccgcct gcgccctcga gcacctcgag gccgcgctcg ccgagcacga ggacacctac
     3421 gacaccacga acccggccgg cagcgacacc acggcgccgg acacgaccgc ccgggacagc
     3481 accacgaccg acaccaccac gagcagcgcg ggctcgggct ccgacaccac cggcgagccc
     3541 ggcaccaccg cctcgacgga cacgaccacg accgccgacc cgggcaccac gaccaccacc
     3601 ggccccccgc ccgtctgcgg cgacggcctg caggaccccg gcgaggagtg cgacgacggc
     3661 aacgccgacc ccatggacgg ctgcaaggac tgcgcccgcg accgcttcat cttcgtcacc
     3721 agcgtcgagt accagggtgg cgagatcgac ggcctctacg gcgccgacca gcgctgtcgc
     3781 acccaggctg ccctcgccaa gctccccaac ttcgccacct accgcgcctg gctcagcgac
     3841 agcaagctcg ctgccgccga ccgcctcctg caccaccccg gccgctacac cctcgtcaac
     3901 ggcctcgtcg tcgccatgga ctgggccgac ctcaccgacg gcaccctcga gaaccccatc
     3961 accgtcaccg agttctccga gtcccccgac agcgtccccg tctggaccgg caccctccac
     4021 aacggcaagt ccgccttcgg cagcagcttt tgcttcgatt ggaccgacac caacagcaac
     4081 tgggatctcg agctgggcgg cagcggcatc cgcacccaga ccgacgccct gtggagctac
     4141 ctcgcgcaag acggctgcgg ttcgaccgcg gcgctctact gcctcgagca gtgacctcga
     4201 ggccccgccc cgtgccccgc atccgcccca tcatcctcgc gctgccgctc gcctgcggcg
     4261 gctcccgcga cgacaccatg aacccggtcg acgacaccat gaacccggcc ggcagcgaca
     4321 ccacggcgcc ggacacgact gcccaggaca gcaccacgac cgacaccacc atcacgagca
     4381 gcgcgggctc gggctccgac accaccggcg agcccggcac cacctcctcg acggacacga
     4441 ccacgaccgc cgacccgggc tccacgacca ccaccaccac cggcaccccg cccgtctgcg
     4501 gcgacggcct gcaggacccc ggcgaggagt gcgacgacgg caacgccgac cccatggacg
     4561 gctgcaagga ctgcgcccgc gaccgcttca tcttcgtcac cag
//
LOCUS       DASWNJ010000403         1732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_183903, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000403 DASWNJ010000000
VERSION     DASWNJ010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183903"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VGB85_06940"
     CDS             complement(<1..653)
                     /locus_tag="VGB85_06940"
                     /inference="COORDINATES: protein motif:HMM:NF028046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133797.1"
                     /translation="MRLLRLGVRGKLFVVSVSLVLAVVLLSGLYLERQLVEWAQTRAQ
                     VELEGHARLVRVLLEREAEVPGPADLDALADRAAAPIDARVTIIAADGRVLGDSEFAA
                     EALATMESHSDRPEVLGALRDGQGVAQRTSASLTTEMLYVALPFPDAGGEGVVRVARP
                     LREVDAAVRRLWIAFTVAGVFAVAGAVLLSGLASHYLSRTLRGLVQDARRMVETGEKS
                     "
     gene            complement(650..1360)
                     /locus_tag="VGB85_06945"
     CDS             complement(650..1360)
                     /locus_tag="VGB85_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0133798.1"
                     /translation="MNGLILIVEDERDLVATLEYALEKEGFRTRSAYTGKQALEIAAT
                     DPGPELVLLDLMLPDMSGTQVCQALRTGERTRNVAVIMMTAKSEEVDRVVGFEVGADD
                     YVVKPFSLRELMLRIRAVLRRKTPAESNAAPSTHGRLRVDMDGHRVWVDEQEVVLTAL
                     EFRLLTTLITRRGRVQTRDALLGDVWGVQPGLTTRTVDTHVRRLRKKLGEVADYVQTL
                     RGVGYRFTTDLREREETP"
     gene            1412..>1732
                     /locus_tag="VGB85_06950"
     CDS             1412..>1732
                     /locus_tag="VGB85_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY0133799.1"
                     /translation="MYKHILAALDGSPRAAAVLQDAALLASRTAATLHLCHAVNVPLG
                     LPADAMSLGGDDLQARLLAHGQQELTLRVEELQPPVTPILWGERLVRLGRPAQVIADV
                     AAELA"
BASE COUNT          244 a          651 c          600 g          237 t
ORIGIN      
        1 gacttctcgc cggtctcgac catgcgccgg gcgtcttgaa ccaggccacg cagcgtgcgc
       61 gagaggtagt gcgacgcgag gccgctcagc agcaccgcgc cggcgaccgc gaacacgccc
      121 gcgaccgtga acgcgatcca cagcctccgc accgccgcgt cgacctcgcg cagcggcctc
      181 gccacgcgca cgaccccctc gccccccgcg tccgggaacg gcagcgcgac gtacaacatc
      241 tcggtcgtca gcgacgcgct ggtccgctgc gcgaccccct ggccgtcgcg cagggcgccg
      301 agcacctcgg ggcggtcgct gtgcgactcc atcgtcgcca gcgcctccgc ggcgaactcc
      361 gagtcgccga gcacgcggcc gtcggcggcg atgatcgtca cgcgcgcgtc gatcggggcc
      421 gcggcccggt ctgcgagcgc gtcgaggtcg gccggtccgg gcacctcggc ctcgcgctcg
      481 agcagcaccc gcaccagacg cgcgtggccc tcgagctcca cctgcgcgcg cgtctgcgcc
      541 cactcgacga gctgacgctc gaggtagagg ccactcagca gcaccaccgc gagcaccagc
      601 gacaccgaga cgacgaacag cttgccgcgc acgccgagcc gcagcaatct catggtgtct
      661 cttcgcgctc gcgcaggtcg gtcgtgaagc ggtagccgac gccgcgcagc gtctgcacgt
      721 agtcggcgac ctcgccgagc ttcttgcgca ggcggcggac gtgggtgtcg acggtgcggg
      781 tcgtcaggcc cggctgcacg ccccacacgt cgccgagcag cgcgtcacgg gtctggaccc
      841 ggccgcggcg ggtgatcagc gtcgtcagca gacggaactc gagcgccgtc agcacgacct
      901 cctgctcgtc gacccacacg cggtggccat ccatgtcgac gcgcaggcgg ccgtgggtgc
      961 tcggcgcggc gttcgactcg gcgggggtct tgcgccgcag caccgcgcgg atccgcagca
     1021 tcagctcgcg caggctaaac ggcttgacca cgtagtcgtc ggcgccgacc tcgaagccga
     1081 ccacgcggtc gacctcctcc gacttggcgg tcatcatgat gacggcgacg ttgcgggtgc
     1141 gctccccggt gcgcagcgcc tggcagacct gggtcccgga catgtcgggc agcatcaggt
     1201 cgagcagcac cagctcgggg ccggggtcgg tcgccgcgat ctcgagggcc tgcttgccgg
     1261 tgtaggccga gcgggtgcga aagccctcct tttcgagggc gtactcgagg gtggccacca
     1321 ggtcgcgctc gtcttcgacg atgaggatca gtccgttcat gggggccacc ccgggtggcc
     1381 cgggagccgg caaatgatac accatggccc catgtacaag cacatcctcg ccgccctcga
     1441 tggatcgccc cgggccgctg cggtgctgca ggacgccgcc ttgctggcgt cacgcaccgc
     1501 cgcgacgtta cacctgtgtc acgccgtcaa cgtgccgctg ggcctgcctg ccgacgcgat
     1561 gagcctcggc ggcgacgatc tccaggcccg cctgctcgcc cacggtcaac aggagctgac
     1621 gctgcgggtc gaggaattgc agccgcccgt gacgccgatc ctctggggcg agcgtctggt
     1681 ccggctcgga cggcccgcgc aggtgatcgc cgacgtcgcc gccgagctgg cg
//
LOCUS       DASWNJ010000404         2723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_183943, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000404 DASWNJ010000000
VERSION     DASWNJ010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183943"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..692)
                     /locus_tag="VGB85_06955"
     CDS             complement(21..692)
                     /locus_tag="VGB85_06955"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY0133800.1"
                     /translation="MELSLLEIWHSMGLMARFVAVALVVMGFLSLFVVLERLLVLMRT
                     SGESLTFARKARLLLDAGEANPLRDLARGFPRNPLARLTVIGLDTYEANIEGKDPIEL
                     TRRELVRKLEVFSAEARRGMGMLASIGSTAPFIGLFGTVIGIITAFQGIASAGGGGLG
                     AVSGGIAEALIVTAVGLVVAIASVLFYNYLSGRFDRLDMQMQHAAGELIDYLEGANGG
                     GSSNQ"
     gene            complement(711..1520)
                     /locus_tag="VGB85_06960"
     CDS             complement(711..1520)
                     /locus_tag="VGB85_06960"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY0133801.1"
                     /translation="MFHGFDHVTRDQVFERRNKRSFLIASLSYLALFGSWIALDRSKE
                     PQVEVMLEPEIKDFAVQEEPEPEVEEEPPPPPPDVAPKPQVQAPKPKPRPEIPKNIPE
                     GPPPEQERPASDKNFGTGQGAGTGTAKPKTDKPPPPKPEVKKPPPPKPAAEPIDPEKP
                     VDRPEKASVPVADAGNKPPAYPGALRDGGVEGEVVVKLHVHRDGTVKGAKILRSTNTA
                     TGEEDKTAADKAFKAAVIAAIKTWKYTPSKLDGSPISVWIIVNFPFKLTAG"
     gene            1697..2488
                     /locus_tag="VGB85_06965"
     CDS             1697..2488
                     /locus_tag="VGB85_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0133802.1"
                     /translation="MRVVDLGCVLGPRALLIDVGFDASDGPLALLGPSGSGKSLTLRC
                     LLGLAPAGARVRGELYLKNDGPPVDLADRRALAALRGRGLTLLPQDAAASLDPVRTVA
                     AQLRELLQVHANREDAPEGLLELVGLRPELLRRYPHEISGGQAQRVALALALACRPAV
                     LLVDEPTAALDNLAQMHAITVLQTTCAAREVDLVFVTHDLALAARVCRHAVIIDSGCI
                     VEADTMEAIVARPAHLTTRGLVAAARRADDLWRLVDGADGREDMS"
     gene            2494..>2723
                     /locus_tag="VGB85_06970"
     CDS             2494..>2723
                     /locus_tag="VGB85_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0133803.1"
                     /translation="MPAMLSARSLGVELGGRAIVEAIDLEIGPGSAVAVVGPSGAGKA
                     ALARALVGLEPALSGSLRFAGVELIGAPREAWR"
BASE COUNT          356 a          923 c         1007 g          437 t
ORIGIN      
        1 gcttgacgcc cttgcccgga ctactggttt gaactgccgc ccccattggc gccctccaga
       61 tagtcgatca gctcgccggc ggcgtgctgc atctgcatgt cgaggcggtc gaagcggccc
      121 gagaggtagt tgtaaaacag caccgaggcg atcgccacga cgaggccgac cgcagtgacg
      181 atcagcgcct cggcgatgcc gccggagacc gcaccgaggc cgccgccgcc cgcggaggcg
      241 atgccctgga acgcggtgat gatgccgatg acggtgccga acaggccgat gaagggcgcg
      301 gtcgagccga tcgacgcgag catgcccatg ccgcggcggg cctcggcgct gaacacctcg
      361 agcttgcgga ccagctcgcg gcgggtcagc tcgatcgggt ccttgccctc gatgttggcc
      421 tcgtaggtgt cgaggccgat gacggtgagg cgggcgagcg ggttgcgcgg gaagccgcgc
      481 gcgaggtccc gcagcgggtt ggcctcaccg gcgtcgagca gcagccgcgc cttgcgggcg
      541 aaggtcagcg actcgccgct ggtgcgcatc aacacgagca ggcgctcgag cacgacgaac
      601 aggctgagga agcccatcac gaccagcgcg accgccacga agcgggccat gaggcccatg
      661 ctgtgccaaa tttcgaggag agagagttcc atgacagtgg tttccccgcc tcagccggcg
      721 gtgagcttga aggggaagtt gacgatgatc cacacggaga tcggcgagcc gtcgagcttc
      781 gagggcgtgt acttccaggt cttgatcgcg gcgatgacgg ccgccttgaa ggccttgtcg
      841 gcggcggtct tgtcttcctc gccggtcgcg gtgttggtcg accgcaagat cttggcgccc
      901 ttgacggtgc cgtcgcggtg cacgtgcaac ttgacgacga cctcgccctc gacgccgccg
      961 tcacgcagcg cgcccgggta cgcgggcggt ttgtttccgg cgtcggcgac cggtacgctg
     1021 gctttctcgg ggcggtcgac gggcttctcg gggtcgatcg gctcggccgc gggcttgggt
     1081 ggcggcggct tcttgacctc gggcttcggc ggcggcggct tgtcggtctt cggcttcgcg
     1141 gtgccggtgc ccgccccctg acctgtcccg aagttcttgt cgctggcggg gcgctcctgc
     1201 tccggcggcg ggccctcggg gatgttcttg gggatctccg ggcgcggctt gggcttgggg
     1261 gcctggacct gcggcttggg ggcgacgtcc ggcggcggcg gaggtggctc ctcctcgacc
     1321 tccggctcgg gctcctcctg cacggcgaag tccttgatct cgggctcgag catgacctcg
     1381 acctgcggct ccttgctgcg gtcgagcgcg atccacgagc cgaacagcgc caggtagctc
     1441 agcgacgcga tgaggaacga acgcttgttg cggcgctcga acacctggtc gcgggtcaca
     1501 tggtcgaagc cgtggaacat cggttcgcgg cgacaatggc gggccgcgtt tgcacggggg
     1561 tggcacttct gttccgtttg cgtgacatcg atcccgggcg cgcatgacat cgcggccgcg
     1621 atcgcgtcac agccaggtca cgaagacccg cagctcgctg tatgcgacgg cgctcgcggt
     1681 gttaccttcg tgacacatgc gagtcgtcga cctcggctgc gtgctcggcc cccgggcctt
     1741 gctgatcgac gtcggcttcg acgcgagcga cggaccactc gcgctgctcg gaccctcggg
     1801 ctccggaaag agcctgacgc tgcgctgcct gctgggcctc gcgcccgcgg gcgcgcgagt
     1861 gcgcggagag ctgtatttaa agaatgatgg accgccggtc gacctcgccg accggcgggc
     1921 cctcgcggcg ctgcggggtc gcgggctgac cctgttaccg caggacgcgg ccgccagcct
     1981 cgacccggtg cgcacggtcg ccgcccagct gcgagaattg ttgcaggtgc acgccaaccg
     2041 cgaggacgcg ccggagggcc tgctcgagct ggtcggcctg cgaccggagc tcttgcgtcg
     2101 gtacccccac gagatcagcg gcggccaggc ccagcgggtc gcgctcgcgc tcgcgctcgc
     2161 gtgccgcccc gcggtcctgc tcgtcgacga accgaccgcg gcgctcgaca acctcgcgca
     2221 gatgcacgcg atcacggtgc tgcagacgac ctgcgcggcg cgggaggtcg acctcgtgtt
     2281 cgtcacgcat gacctcgcgc tcgccgcccg cgtgtgccgg cacgcggtga tcatcgacag
     2341 cggctgcatc gtcgaggccg acacgatgga ggcgatcgtc gcccggccgg cgcacctgac
     2401 gacgcgcggc ctggtcgccg cggcccgccg cgccgacgac ctgtggcggc tggtcgacgg
     2461 cgcggatggc cgggaggaca tgtcgtgaag gacatgcccg ccatgctctc ggcgcgcagc
     2521 ctcggggtgg agcttggcgg tcgcgcgatc gtcgaggcga tcgacctcga gatcgggccg
     2581 ggctcggcgg tcgcagtcgt cgggccgtcc ggcgccggca aggcggcgct cgcgcgcgcg
     2641 ctcgtcggcc tcgaaccggc gctctcgggc tcgctgcgct tcgctggtgt cgagctgatc
     2701 ggcgcgccgc gcgaggcgtg gcg
//
LOCUS       DASWNJ010000405         2319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_431487, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000405 DASWNJ010000000
VERSION     DASWNJ010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431487"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..1486)
                     /locus_tag="VGB85_06975"
     CDS             complement(227..1486)
                     /locus_tag="VGB85_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133804.1"
                     /translation="MKRLQRRIGLAALLTCPLGQGCSPLIYEIATQMDSEHMGPAGDT
                     GMPTDPTLPDPTDATDDTGPSSTTDAIPSTSTGPETTGAPPACGDGMHNGDETDLDCG
                     GSCKPCGPGGRCQTPADCAFGDCLDNFCQKAECIGPEDCPPALPCMGSMCTKDGRCAA
                     VPAGDGEPCDDGDACTSAAVCKGGMCVETSQVDCSAFDGPCRAAVCNPNTGNCAVEWL
                     FEGEPCEDGLGCTWFDQCSQGECVGKPPGPLLSTDFSDALGWKADAPWKIGPAMPSQC
                     SLGPAEDPKDDHSPGGDGILAGAAIGGCLPVDAFADVCLESPPVDVMFMPGELRLTYW
                     SVLSSAGAPMKTRVEVFDGKNQQWLPIEVFDKFIAEGDWTEHDLDLTPFKGPALRVRF
                     CHAAADKSQPVGGWSIDDLSIGAPQCP"
     gene            complement(1486..>2319)
                     /locus_tag="VGB85_06980"
     CDS             complement(1486..>2319)
                     /locus_tag="VGB85_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133805.1"
                     /translation="LTARGHAELGALLAEARSEVDAELAALRRKGWPPVPVPKASPQR
                     RRWIPWLSLAAAAVLIAVASPMLATRFEAARDSNQAPWWQRTGDTTREAVRREATVTP
                     APVSPRPALAEPPATPVEPEAPVEPAPIVEAPAEQPVPKVRKPAPPPAAAETLAARLR
                     RLGAEAEQAWQSGDPAGAERRHREIIALAPGSRAADLSYGDLFTLARQRSGSEQETSL
                     WREYLTAFPRGRYADDARAGLCRRAAPDERAACWQQYLTDFPAGVHRAQATRARGDAT
                     P"
BASE COUNT          292 a          788 c          906 g          333 t
ORIGIN      
        1 cttgcatctt cctcggcgag agaacgatcg ctcgtcgcgc tgtcgcaggc ggggacgaac
       61 agaccgatca gcgcgaagcc gacgaccgac agaccagaac gagagatcac agactgacta
      121 cgcatgggtc acactccttg agagttcttc aactcggggt tcgtcggtga gtgacccgag
      181 cccggaggcg attacagcgg cgtcgtaaat attttctctg cgacgatcac gggcactgcg
      241 gcgcgccgat gctgaggtcg tcgatgctcc agccgccgac cggctgactc ttgtcggcgg
      301 cggcgtggca aaagcgcacg cgcagggccg ggcccttgaa tggcgtgagg tcgaggtcgt
      361 gctcggtcca gtcgccctcg gcgatgaact tgtcgaacac ttcgatcggg agccactgct
      421 ggttcttgcc gtcgaagacc tcgacgcggg tcttcatcgg cgcgcccgcg ctgctgagca
      481 cgctccagta agtgaggcgc agctcgccgg gcatgaacat gacgtcgacc ggcgggctct
      541 cgaggcagac atcggcgaac gcgtcgaccg gcaggcagcc gccgatcgca gcgcccgcga
      601 ggatgccgtc gccgccgggc gagtggtcgt ccttggggtc ctcggcaggg ccgaggctgc
      661 actgcgaggg catggccggg ccgatcttcc acggagcatc cgctttccag ccgagcgcgt
      721 cgctgaagtc ggtcgacagc agcgggcccg gcggcttgcc gacgcactcg ccctggctac
      781 actggtcgaa ccaggtgcag ccgaggccgt cctcgcaggg ctcgccctcg aacaaccact
      841 cgaccgcgca attaccggtg ttcgggttgc agacggccgc gcggcacggg ccgtcgaagg
      901 cgctgcagtc gacctgcgag gtctcgacgc acatgccgcc cttgcacacc gccgcgctgg
      961 tgcacgcgtc gccgtcgtca cagggctcgc cgtcgcccgc agggaccgcg gcgcagcggc
     1021 catccttggt gcacatcgag cccatgcacg gcagcgcggg cgggcagtcc tccgggccga
     1081 tgcactcggc cttttggcag aagttgtcga ggcaatcgcc gaaggcgcag tcggcggggg
     1141 tctggcagcg cccgcccgga ccgcagggct tgcagctgcc gccgcagtcg aggtcggtct
     1201 cgtctccgtt gtgcatgccg tcgccgcagg ccggcggcgc accggtcgtc tcggggccgg
     1261 tcgaggtcga ggggatcgcg tcggtcgtgc tgctcggccc ggtgtcgtcg gtcgcgtcgg
     1321 tcgggtcggg cagggtcggg tcggtgggca tgccggtgtc cccggcgggg cccatgtgct
     1381 cgctgtccat ctgcgtggcg atctcgtaga tcagcggcga gcagccctgt cccaggggac
     1441 aggtcagcag cgcggcgagg ccgatgcgtc gctgcagtcg cttcatcatg gcgtggcgtc
     1501 gccccgggcc cgcgtggcct gggcccggtg gacgcccgcg gggaagtcgg tcaggtactg
     1561 ctgccagcac gcggcgcgct cgtcgggcgc ggcgcggcgg cacaggccgg cgcgcgcgtc
     1621 gtcggcataa cgcccgcgcg ggaaggcggt caggtactcg cgccacagcg aggtctcctg
     1681 ctcggagccg gagcgctggc gggcgagcgt gaacaggtcg ccgtagctga ggtcggcggc
     1741 gcggctgccg ggcgcgagcg cgatgatctc gcggtggcgt cgctcggccc cggccgggtc
     1801 gcccgactgc caggcctgct ccgcctcggc gccgagccgg cgcagccgcg ccgccagcgt
     1861 ctcggcggcg gcgggcggcg gcgcgggctt cctgaccttc gggacaggtt gctcggcggg
     1921 cgcctcgacg atgggcgcgg gctcgacggg cgcctcgggc tcgacgggcg tcgccggcgg
     1981 ctcggcgagc gcggggcgcg gggacacggg ggccggcgtg acggtggcct cgcgccgcac
     2041 ggcctcgcgg gtggtgtcgc cggtgcgttg ccaccacggg gcctggttgc tgtcgcgggc
     2101 ggcctcgaag cgggtcgcga gcatcggcga ggcgacggcg atcagcacgg cggcggcggc
     2161 gagcgagagc caggggatcc agcggcgtcg ctgcgggctg gccttcggga caggtaccgg
     2221 gggccagccc ttgcggcgca gggcggccag ctcggcgtcg acctcgctgc gggcctcggc
     2281 gagcagggcg ccgagctccg cgtggccgcg ggcggtcag
//
LOCUS       DASWNJ010000406         3623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_431508, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000406 DASWNJ010000000
VERSION     DASWNJ010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431508"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VGB85_06985"
     CDS             <1..295
                     /locus_tag="VGB85_06985"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase / HUMPS
                     family protein"
                     /protein_id="HEY0133806.1"
                     /translation="RAAAAAAAGCAGLVCSGQELARVREAAPGLVYVVPGIRPAGGDT
                     GDQKRVMTPGEAIAAGAGYLVVGRPILGAADPAAAADAIVAEIAGLNKIERAH"
     gene            408..797
                     /locus_tag="VGB85_06990"
     CDS             408..797
                     /locus_tag="VGB85_06990"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEY0133807.1"
                     /translation="MNQDPRPSLYERLGGVYAIAVVVDDFIDRIMTDPRLNANPLVDE
                     AHHRVTPPGFKYLVTEMVCWATGGPQQYSGKNMKDSHAHLKITAAEWDAFAEDFATTL
                     AKFNVPARESEELFAILGSTRGDIVTA"
     gene            complement(801..1547)
                     /locus_tag="VGB85_06995"
     CDS             complement(801..1547)
                     /locus_tag="VGB85_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0133808.1"
                     /translation="MSEARNFRIAALSDIHVTKDSRGQLSPIVQQAAVGADVMIICGD
                     LTDYGTIEEAEVLVRELASVQIPILTVLGNHDFESGHGAEVTAILREAGVKVLDGEAT
                     EVHGVGFAGIKGFCGGFGRHALGPWGETAIKSFVREALDEALKLEAALARLRTPQKIA
                     ILHYSPIIATVEGEPLEIYPFLGTSRLEEPLVRYPVNAVFHGHAHRGSPEGQLSNGTP
                     VYNVALPLLRRLAPDGPWYRTLDLVIDPTA"
     gene            complement(1559..2356)
                     /locus_tag="VGB85_07000"
     CDS             complement(1559..2356)
                     /locus_tag="VGB85_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133809.1"
                     /translation="MTAPASPTKDLVDPQARAFYVRALNALNESGIPYLVGGAYAFAR
                     YTGIERHTKDLDLFVRPDDARKILAALATVGYQGDMTFPHWLGKAFCGEYFVDIIFGS
                     GNNIARVDDVWFEHAVDEVVLGVKTRLIPPEEMMWSKGYIMERERFDGADIAHVLRAC
                     AERLDWDRLLARFHTHWQVLLSHLVMFGFIYPGERQRIPQRVMDSLVRRLSEPADAAK
                     VCQGTLLSRQQYLTDIGTWGYADARLTQNLMTPEDIAHWTAGIEAAH"
     gene            complement(2511..>3623)
                     /locus_tag="VGB85_07005"
     CDS             complement(2511..>3623)
                     /locus_tag="VGB85_07005"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0133810.1"
                     /translation="DPPDSSGAPTGGPPAPGVCGDGVVHDGEACDDGNDVPDDGCDAI
                     CAKTAALEWSYAPIDVPFANDLTVGPEGRIVVVGDGIVIALEPDGSEVWRKTMPVESH
                     AFNSVEIDEQGTIYVAGEVGTMHALAPNGDELWNLAMETGFQAIALGHGALYSVDLDA
                     PSDRLIVRRHDRSTGAVQWATQSPEGISTYPYGMAVVGPNIIVAGMALIGLGSPTQPL
                     LAVYDDSGVEQSFTLGADEGRRWTAVTAADDGFVLAGFGPEADIVLSRLDAGLVPQWT
                     VYDEGAFGTQANSVASGAGGDLVIAAHDLAERAGLVRRFDAAGATVWTAVFPEDGGHT
                     QYPNTAAFGPDFIVVLGNAWSGGGPHGMWVRRFALN"
BASE COUNT          540 a         1186 c         1356 g          541 t
ORIGIN      
        1 gcgggcggcg gcggcggcgg cggcgggctg cgcggggctg gtgtgcagtg gccaggagct
       61 cgcgcgcgtc cgcgaggcgg cgccgggcct ggtctacgtg gtgccgggga tccgcccggc
      121 cggcggcgac accggcgacc agaagcgcgt gatgacgcca ggcgaggcga tcgctgccgg
      181 ggccgggtat ctcgtcgtcg gccggccgat cctcggggcg gccgatccgg ccgcggccgc
      241 cgacgcgatc gtcgcggaga tcgccgggct gaacaagatc gagcgcgcgc actagagcct
      301 gtccatcggg acaggttcga gaacgtcggt ccacggtgag tgggccccga atcggcgcat
      361 cggcgggggc ttgctcggcc gaacccggtc gtgctcccct cgcgtggatg aatcaggatc
      421 ctcgtccttc cctgtatgag cgtctcggtg gggtctatgc gatcgccgtg gtcgtcgacg
      481 acttcatcga ccgcatcatg accgacccgc ggctcaacgc gaacccgctc gtcgacgagg
      541 cgcaccaccg ggtcacgccg ccgggcttca agtacctggt caccgagatg gtgtgctggg
      601 cgaccggcgg gccgcagcag tacagcggca agaacatgaa ggactcgcac gcgcacctga
      661 agatcacggc cgccgagtgg gacgcgttcg ccgaggactt cgcgaccacg ctggcgaagt
      721 tcaacgtgcc cgcccgcgag agcgaggagc tgttcgcgat cctcggcagc acccgcggcg
      781 acatcgtcac cgcgtgagag ctaggcggtc gggtcgatca cgaggtcgag ggtgcggtac
      841 cacgggccgt ccggcgcgag ccggcgcagc agcggcaggg cgacgttgta gacgggtgta
      901 ccgttgctga gctggccttc gggcgagccc cggtgcgcgt ggccgtggaa cacggcgttg
      961 accgggtagc gcacgagcgg ctcctcgagc cggctggtgc cgaggaacgg gtagatctcg
     1021 agcggctcgc cctcgacggt ggcgataatc ggggagtagt ggaggatggc gatcttctgc
     1081 ggggtgcgca ggcgggcgag cgccgcctcg agcttgaggg cctcgtcgag ggcctcgcgc
     1141 acgaagctct tgatcgcggt ctcgccccac ggcccgagcg cgtggcgacc aaagccgccg
     1201 caaaagccct tgatgccggc gaagccgacg ccgtggacct cggtggcctc gccgtcgagg
     1261 accttgacgc cggcctcgcg caagatcgcg gtgacctcgg cgccgtgccc ggactcgaag
     1321 tcatgattgc cgagcacggt gaggatcggg atctgcacgc tggcgagctc gcgcaccagc
     1381 acctcggcct cctcgatcgt gccgtagtcg gtgaggtcgc cgcagatgat catcacgtcg
     1441 gcgccgacgg ccgcctgttg aacgatcggc gagagctgcc cgcgcgagtc cttggtgacg
     1501 tggatgtcgc tgagcgcggc gatgcgaaag ttacgagctt cggacatggg atcctccgtc
     1561 agtgcgcggc ctcgatgccg gcggtccagt gggcgatgtc ctcgggcgtc atgaggttct
     1621 gcgtcagccg ggcgtcggcg tagccccagg tgccgatgtc ggtcaggtac tgctggcgcg
     1681 acagcaaggt gccctgacag accttggcgg cgtcggccgg ctcgctcagg cgccgcacca
     1741 ggctgtccat cacgcgctgg gggatccgct ggcgctcacc ggggtagatg aagccgaaca
     1801 tcacgaggtg cgagagcagc acctgccagt gggtgtggaa gcgggcgagc aggcggtccc
     1861 agtcgaggcg ctcggcgcag gcgcgcagca catgggcgat gtccgccccg tcgaagcgct
     1921 cgcgctccat gatgtagccc ttgctccaca tcatctcctc gggcgggatc aggcgggtct
     1981 tgacgccgag cacgacctcg tcgaccgcat gctcgaacca tacgtcgtcg acgcgggcga
     2041 tgttattgcc ggagccgaag atgatatcga cgaaatactc gccgcaaaac gccttgccga
     2101 gccagtgcgg gaaggtcata tccccctggt agccgacggt ggccagcgcc gcgaggatct
     2161 tgcgcgcgtc gtccgggcgg acgaacaggt cgaggtcctt ggtgtggcgc tcgatgccgg
     2221 tgtaacgcgc gaaggcgtag gcgcccccga ccagatacgg gatgccggac tcgttgaggg
     2281 cgttgagcgc acgcacgtag aaggctcgtg cttgcgggtc gacgaggtcc ttggtcgggg
     2341 atgctggcgc ggtcacgggg ttctccgaac cgcgaagtat gcagcgggcc gcgcgggcga
     2401 gcgccggcgc ggcctgcctg tgcgtccggg ggcgaggaag gggcgcccac gcggtcctgc
     2461 gagcgatctg cggggggccc gcggctgggc tttggtagga cttcgggcga tcagttgagc
     2521 gcgaaccggc ggacccacat accgtggggt ccccccccgg accaggcgtt gccgagcacg
     2581 acgatgaagt ccggcccgaa cgcggcggtg ttggggtact gcgtgtgtcc tccgtcctcg
     2641 gggaagacag cggtccacac ggtcgcgccg gcagcgtcga agcggcgcac gagcccggcg
     2701 cgctccgcga ggtcgtgggc ggcgatcacg aggtcacccc cggctccgct ggcgactgag
     2761 ttcgcctggg tgccgaacgc gccctcgtcg tagacggtcc actgcggcac gagcccggcg
     2821 tcgaggcggc tgaggacgat gtccgcctcg ggtccgaagc cggcgagcac gaacccgtcg
     2881 tccgcggcgg tgaccgccgt ccaccggcgg ccctcgtcgg cgccgagcgt gaacgactgc
     2941 tcgacgccag aatcgtcgta cacggcgagg agcggctggg tggggctgcc caggccgatc
     3001 agcgccatgc cggcgacgat gatgttcggg ccgaccacgg ccatgccgta gggatatgtg
     3061 ctgatcccct cgggcgactg ggtggcccac tggaccgcgc ccgtgctgcg gtcgtgccgg
     3121 cggacgatga gccgatcgga gggcgcgtcg aggtcgaccg aatacagcgc gccatgaccg
     3181 agcgcgatcg cctggaaacc agtctccatt gcgaggttcc acagctcgtc gccgttcgga
     3241 gccagggcgt gcatcgtgcc gacctcgccg gcgacgtaga tcgtgccctg ctcgtcgatc
     3301 tcgaccgagt tgaaggcgtg cgattcgacg ggcatggtct tgcgccacac ctcgctgccg
     3361 tccggctcga gcgcgatcac gatgccgtcg ccgacgacga cgatgcgccc ctccgggccg
     3421 acggtgaggt cgttcgcgaa cgggacatcg atgggcgcgt aggaccactc gagcgccgcg
     3481 gtcttcgcac agatggcgtc gcagccgtcg tccggcacat cgttgccgtc gtcacacgcc
     3541 tcgccatcgt ggacgacgcc gtcgccacaa acgccggggg ccggtggtcc gccggtcgga
     3601 gcgccggatg aatcgggtgg atc
//
LOCUS       DASWNJ010000407         5715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_183977, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000407 DASWNJ010000000
VERSION     DASWNJ010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5715
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183977"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VGB85_07010"
     CDS             <1..620
                     /locus_tag="VGB85_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133811.1"
                     /translation="NDSASITRVKMDEMRQVPVGGTSRDFTAVLDLSPTAMRDAAGVR
                     LASTSSAESKYIVDGMSMAGTRQVPEGYVPGEVRRLEKSMQYATPGPRRGERVEVHAR
                     AKLRSIRGVEGAQGEVLRQGLSAGMERLSMCFENAPRATYRVRRGVTLRVVFAANGKI
                     HSLRIAGSGTGDPALQQCLRDRLTPLLAGTTSKQLTLELRVAMQF"
     gene            complement(624..3671)
                     /gene="ligD"
                     /locus_tag="VGB85_07015"
     CDS             complement(624..3671)
                     /gene="ligD"
                     /locus_tag="VGB85_07015"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEY0133812.1"
                     /translation="MSLDRYNEKRDFKKTPEPAGSVVKNKGALRFVIQKHAATRLHYD
                     FRLELGGVLLSWAVPKGPSLNPADKRLAVHVEDHPLEYATFEGSIPHGEYGGGSVVVW
                     DRGHWFPEGDPENDYKRGNLKFRLDGEKCQGSFALVRMHGPRSDDGKNWLLLKHADEH
                     ASEDGELLVRDKPESVQTGRTVEEVAAAPDKAWHSNRGGQKGGEKVLSAAESKSLAIK
                     PKATKKPAVAAEELDPEEVARRRLGLAPRAAARTAKPAKKTAAKKVTRTAAKKATAKK
                     VTKAATAKKATAKKVTKATTTKKAAAKKVTKATTTKKAAAKKTAAKKVAKATGKASAK
                     KAAKRISRAAEEPAPPVRATPPRGRLGESHLPEKIAPELATLVEAVPPGPEWLHEVKY
                     DGYRLVARIDGDHVALLTRGGEDWSAKFPWLVAALKTRVDVAQAILDGELVHLGSDGV
                     TRFGELQKALSEDRHESLVYFVFDVLHLQGLDLRDLPLDQRKQALADLLPPEKKSGRV
                     RLSEHIVGQGEPLITRACKIGLEGVISKRRDAPYKSGRHRDWLKIKCGKRQEVVVGGF
                     TPARSGNRDIGSLLLGLYDEAGNFIYCGKVGSGFDFSGATKLRARLDKLVTRQSPFSK
                     MPPGFSRNTFVRPEIVVEVSFTDWTEDGRMRHPVFEGVREDKPAKTIRRETSMPVAKA
                     VAAVSAPAPATATKAAAKQVLVSKPASSPSKRRGEESEVLGVRVSHPSRVLYPADGIT
                     KGEIAEYYAATAGWMMPYTGHRPISVVRCPDNIEKPCFFQKHIHHEMPPGVATADLDG
                     EGGDAPFIYVDTAQGLVGLTQVGTLEVHIWGSTIERPEDPDRIIIDLDPDEKVPWARV
                     KETAAAVRTRLEDLGLTSFLKTTGGKGLHVVAPLTPGKQDWDTVKAFTHGIALEFARA
                     APDLFTATATKAKRTGRIYIDYLRNYRGSTSVAPYSTRSRPGAPVSVPLRWDELAALE
                     SSYKYTIRNLAARLKSLKADPWKDMLKTKQHLKPEVLRAVIKK"
     gene            complement(3678..4649)
                     /locus_tag="VGB85_07020"
     CDS             complement(3678..4649)
                     /locus_tag="VGB85_07020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEY0133813.1"
                     /translation="MATRAIWEGTISFGLVQIPVGLHTAESRDDLDFDMLDRRDMAHV
                     GYQRFNRNTNEPVDWDNIVKGYEYEPGEYVIVEKEDFKRANVKATQTVDIVHFTDASN
                     IDVMYFEKPYYLVPSKKGHKAYVLLREVLRRSNKVGIARVVIRTRENVAAVMVRGNAL
                     ILEILRYDHELHKPSDYAIPEESVKQMNISPGELKMAERLVSDMEAPFKPEEFKDTYR
                     DDLLAFIKEKIKHGDVEEPEQPEEAPEASNVVDIMELLRNSLGKRGGAANEKPGKASS
                     KKAAKKTTAAKKTTKKAAKKATKKTAAKKTGKKAAKKTTHAPTRKAS"
     gene            complement(4733..5146)
                     /locus_tag="VGB85_07025"
     CDS             complement(4733..5146)
                     /locus_tag="VGB85_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133814.1"
                     /translation="MRTRDPEETTPERLRSLLRGEMSAAETYERALERASGSPARARL
                     REICADHSQAVGLLRDLLIRYPGELPTNSGAADVPCETVALTTLKQAEEHGIRGYEQV
                     LRDPEVAEDAKAMSRSLLDRCRSHVPVLDELIDHE"
     gene            5527..>5715
                     /locus_tag="VGB85_07030"
     CDS             5527..>5715
                     /locus_tag="VGB85_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133815.1"
                     /translation="MRDQDDQDLDPRMRERLAALEQAVIEERGRHAAAEADARRYRMF
                     LDSVQDYAFITFSDALRVV"
BASE COUNT          730 a         1885 c         2018 g         1082 t
ORIGIN      
        1 tcaacgactc ggcatcgatc accagggtga agatggacga gatgcggcag gtcccggtcg
       61 gcggcacctc gcgcgacttc acggccgtgc tcgacctgtc gccgacggcg atgcgcgacg
      121 ccgcgggggt ccgcctcgcc agtaccagca gcgcggagtc gaagtacatc gtcgatggca
      181 tgagcatggc cggcacgagg caagtccccg agggctacgt gcccggggag gtccggcggc
      241 tcgagaagtc gatgcaatac gcgacaccgg ggccgaggcg cggcgagcgg gtcgaggtcc
      301 acgcgcgcgc caagctgcgg tcgatccgcg gggtcgaggg cgcgcagggc gaggtgctgc
      361 gccagggcct gtcggccggc atggagcgcc tctcgatgtg ttttgaaaat gccccgcggg
      421 cgacctaccg cgtgcgccgt ggcgtcaccc tgcgggtcgt gttcgccgcg aacggcaaga
      481 tccacagcct gcggatcgca ggttccggga ccggggatcc cgcgctgcag cagtgcctgc
      541 gcgatcgcct gaccccgctg ctcgccggca ccaccagcaa gcagctgacg ctggagctgc
      601 gggtggcgat gcagttttga cggctatttc ttgatcaccg cgcgcagcac ctcgggtttg
      661 aggtgctgct tggtcttgag catgtccttc caggggtcgg ccttgaggct cttgaggcgc
      721 gccgcgaggt ttctgatcgt atacttataa gagctctcga gcgcggcgag ctcgtcccag
      781 cgcagcggga ccgacaccgg ggcgccgggg cgtgagcggg tcgagtaggg ggcgacgctg
      841 gtcgagccgc gatagttgcg cagataatcg atgtagatcc ttccggtgcg cttggccttg
      901 gtcgcggtcg cggtgaacag gtcgggcgcg gcgcgggcga actcgagggc gatgccgtgc
      961 gtgaacgcct tgacggtgtc ccagtcctgc ttgccgggcg tcagcggcgc gacgacgtgc
     1021 agacccttgc cgccggtggt cttgaggaag ctggtcaggc cgaggtcctc gaggcgcgtg
     1081 cgcaccgcgg cggcggtctc cttgacgcgg gcccacggga ccttctcgtc ggggtcgagg
     1141 tcgatgatga tgcggtcggg gtcctcgggg cgctcaatgg tagaacccca gatgtggacc
     1201 tcgagcgtgc caacctgcgt caggccgacg aggccctgcg cggtgtcgac gtagatgaag
     1261 ggggcatcgc cgccctcgcc gtcgaggtcg gccgtggcga caccgggcgg catctcgtgg
     1321 tgaatgtgct tctgaaagaa gcacggcttt tcaatgttgt ccgggcagcg caccacggag
     1381 atcggtcggt gcccggtgta cggcatcatc cacccggcgg tcgcggcgta gtactcggcg
     1441 atctcgccct tggtgatgcc gtcggccggg tagagcacgc gcgaggggtg cgagacgcgg
     1501 acgccgagga cctcggactc ctcgccgcgg cgcttgctcg gcgagctcgc cggcttgctg
     1561 acgagcacct gcttcgcggc ggccttcgtc gcggtggcgg gcgcgggggc cgagaccgcg
     1621 gcgaccgcct tggcgaccgg catcgaggtc tcgcggcgga tcgtcttcgc cggcttgtcc
     1681 tcgcgcacgc cctcgaacac cgggtgacgc atgcggccgt cctcggtcca gtctgtaaaa
     1741 ctgacctcga cgacgatctc cgggcgcacg aaggtgttgc gcgagaaccc gggcggcatt
     1801 tttgaaaagg gggattgccg ggtgacgagc ttgtcgaggc gggcgcgcag cttggtcgcg
     1861 ccggagaagt cgaagccgct gccgaccttg ccgcaatata tgaaattgcc ggcctcgtcg
     1921 tagaggccga gcagcagcga gccgatgtcg cggttgccgc tgcgcgccgg cgtgaagccg
     1981 ccgacgacga cctcctggcg cttgccacac ttgatcttga gccagtcgcg gtggcggccc
     2041 gacttgtagg gcgcgtcgcg tcgcttggag atcacgccct cgagcccgat cttgcacgcg
     2101 cgggtgatca gcggctcgcc ctgcccgacg atgtgttctg ataagcgaac gcggcccgat
     2161 tttttctccg gcggcagcag gtcggcgagc gcctgcttgc gctggtcgag cgggaggtcg
     2221 cgcaggtcga gaccttgcag gtgcaacacg tcgaacacga agtatacgag cgactcgtgg
     2281 cggtcctcgg acagcgcctt ttgcagctcg ccgaagcgcg tcacgccgtc gctgccgagg
     2341 tgcacgagct caccgtcgag gatcgcctgc gcgacgtcga cgcgggtctt gagcgcggcg
     2401 acgagccacg ggaatttcgc gctccagtcc tcaccgccgc gcgtcagcag ggcgacgtgg
     2461 tcgccgtcga tgcgcgcgac caggcgataa ccatcgtact tgacctcgtg cagccactcc
     2521 gggcccggcg gcacggcctc gaccaaggtc gccagctcgg gggcgatctt ctcggggagg
     2581 tgcgactcgc cgaggcggcc gcgcggcggc gtggcgcgga cgggaggggc aggctcctcg
     2641 gcggcgcggc tgatccgctt cgcggctttt ttcgccgagg ctttgccggt cgccttcgcg
     2701 accttcttgg cggcggtctt cttggcggcg gccttcttcg tggtcgtggc tttcgtgacc
     2761 ttcttggcgg cggccttctt cgtggtcgtg gctttcgtga cctttttcgc ggtcgccttc
     2821 ttcgccgtcg cggccttggt gaccttcttc gcggtcgcct tcttcgcggc ggtcctggtg
     2881 accttcttcg cggccgtctt cttcgccggc ttcgcggtgc gggcggcggc gcgcggggcc
     2941 aggccgaggc ggcggcgcgc gacctcctcg gggtcgagct cctccgcggc cacggcgggc
     3001 ttcttcgtcg ccttgggctt gatcgcgagg ctcttgctct ccgcggcgct caggaccttc
     3061 tcgccgcctt tttggccgcc gcggttgctg tgccaggcct tgtcgggggc ggcggcgacc
     3121 tcttccacgg tgcggccggt ctgcacgctc tcgggtttgt cgcggaccag cagctcgccg
     3181 tcctcgctcg cgtgctcgtc ggcgtgcttg agcagcagcc agttcttgcc gtcgtcgctg
     3241 cgcgggccgt gcatacgcac cagcgcgaag ctgccctggc acttctcgcc gtcgaggcga
     3301 aacttgaggt tgccgcgctt gtagtcgttc tcggggtcgc cctcggggaa ccagtggccg
     3361 cggtcccaca cgaccaccga gccgccgccg tactcgccgt gcgggatgct gccctcgaag
     3421 gtcgcgtact ccagcgggtg gtcctcgacg tgcacggcga ggcgcttgtc ggcggggttg
     3481 aggctcgggc ccttcgggac cgcccagctg agcagcacgc cgccgagctc gaggcgaaag
     3541 tcatagtgga gccgggtggc cgcgtgcttc tggatcacga agcgcagcgc gcccttgttc
     3601 ttgacgacgc tgccggccgg ctccggcgtc tttttgaagt cgcgcttctc gttgtaacga
     3661 tcgagcgaca tggcggccta gctcgccttc cgcgtcggcg cgtgggtcgt cttcttggcg
     3721 gccttcttgc cggtcttctt ggcggcggtc ttcttggtcg ccttcttggc ggccttcttg
     3781 gtcgtcttct tggcggcggt cgtcttcttg gcggccttct tgctcgacgc cttgccgggc
     3841 ttctcgttgg ccgcgccgcc gcgcttgccg aggctgttgc gcagcagctc catgatgtcg
     3901 acgacgttgc tcgcctcggg cgcctcctcg ggctgctcgg gctcctcgac gtcgccgtgc
     3961 ttgatcttct ctttgataaa ggccagcagg tcgtcgcggt aggtgtcttt gaactcctcc
     4021 ggcttgaacg gggcctccat gtcggagacg aggcgctcgg ccatcttgag ctcgcccggc
     4081 gagatgttca tctgcttgac gctctcctcc gggatcgcgt agtccgaggg cttgtgcagc
     4141 tcgtggtcgt agcgcaggat ctcgaggatc agcgcgttgc cgcggaccat caccgcggcg
     4201 acgttctcgc gggtgcggat cacgacgcgg gcgatgccga ccttgttgga cctccgcagc
     4261 acctcgcgca gcagcacgta cgccttgtgg cccttcttgc tcgggaccag gtagtagggc
     4321 ttctcgaagt acatcacgtc gatgttgctg gcatcggtga agtgcacgat gtcgaccgtc
     4381 tgcgtcgcct tgacgttggc gcgcttgaag tcctccttct cgacgatgac gtactcgccc
     4441 ggctcgtact cgtagccctt gacgatgttg tcccagtcga ccggctcgtt ggtgttgcgg
     4501 ttgaatcgct ggtacccgac gtgggccatg tcccgccggt cgagcatgtc gaagtccaga
     4561 tcgtcgcggg actcggcggt atgcaggccc acggggatct gcaccaaacc gaagctgatc
     4621 gtaccttccc aaattgctct ggtcgccatg tgcgtgcgcc gccttcgcgc ggttggttcg
     4681 tatcaccaaa ccgagcatcg gcaagcgcgc cggtccacta ccagcattca tctcattcgt
     4741 ggtcgatcag ctcgtcgagc accggcacgt ggctgcgaca ccggtccagc aggctgcggc
     4801 tcatcgcctt ggcgtcctcg gcgacctcgg ggtcgcgcag cacctgctcg tagccgcgga
     4861 tcccgtgctc ctcggcctgc ttgagggtcg tcagggcgac cgtctcacac ggcacgtcgg
     4921 cggcccccga gttggtcggc agctcgcccg ggtagcggat cagcaggtct cgcagcagcc
     4981 cgaccgcctg gctgtggtcg gcgcagatct ctcgtagcct ggcccgggcc ggagatccgc
     5041 tggctcgctc gagcgcccgc tcgtaggtct cggcggcgga catctcgccg cgcagcaggc
     5101 tccggagccg ctcgggggtg gtctcttcgg ggtctcgcgt tcgcatggtc ggtgtctcct
     5161 cgcggatcga atggggggca tgcgaccacg ggcacgacgc cacgtatgaa tcgcaatgca
     5221 cgcgcggtgc ctctgctccg cgccgctccg tgtctgcgga gcccctgcgc cacgtacgcg
     5281 taatcgtagt tcttgggtca tacccacgcg tgcggccgtg gcaaggccgg cccgcacctc
     5341 gtcgcaccta cacagggttc tcccggcgga ctgtcccggg cgatcgatgc tgcgcagcgg
     5401 ccgctcgatc gggcgcagtt ttctcaggaa tggcagcaac ttcatcgaag tgtcgcggac
     5461 tgctggcgaa ccggcacgcg tgtgccatag ctcgttgcac tccggcgccg gagagccagg
     5521 gaggccatgc gagaccagga cgaccaggac ctcgacccgc ggatgcgcga gcgcctcgcc
     5581 gcgctcgagc aggcggtgat cgaggagcgc gggcgccacg ccgcggcaga ggccgacgcg
     5641 cggcgctatc gcatgttcct cgacagcgtg caggactacg cgttcatcac cttcagcgac
     5701 gcgctgcggg tggtc
//
LOCUS       DASWNJ010000408         2774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_538057, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000408 DASWNJ010000000
VERSION     DASWNJ010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538057"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VGB85_07035"
     CDS             complement(<1..360)
                     /locus_tag="VGB85_07035"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133816.1"
                     /translation="MFAAPERHGLRVTRGVLCVEGVSLADIAAAVGTPTYVYGSAEIG
                     GRFDELTRACAGHPSLLCYAVKANSSQAVLRTLARRGAGADIVSGGELARALAAGIPG
                     ARIVFSGVGKLDPEIDAA"
     gene            complement(1056..1688)
                     /gene="ribA"
                     /locus_tag="VGB85_07040"
     CDS             complement(1056..1688)
                     /gene="ribA"
                     /locus_tag="VGB85_07040"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEY0133817.1"
                     /translation="MPVPHSLSVRAPKVLRGVNVFARARLPSRHGEFEIVSFIDEAGR
                     TLDDVAIVRGELDGAAEVTTRIHSECLTGDVFGSYRCDCRDQLELAMARISTGERGII
                     LYLRQEGRGIGIAQKVRAYQLQQTGLDTVEANLHLGFDDDLRDYSVAAAMLHALGVVS
                     VVLHTNNLRKIEGLRAGGVEVHRREAIIAESREQNVGYLATKRDKSGHLL"
     gene            complement(1724..2488)
                     /locus_tag="VGB85_07045"
     CDS             complement(1724..2488)
                     /locus_tag="VGB85_07045"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEY0133818.1"
                     /translation="MTPARTRNYRQIFESSELLWEKLGMLSPAQRRSFDERFIMSWIY
                     HDFALEGTVLSVAEIKAAIDDEIISTPSLIPAYYHIMAVRDAIQFLLDSRGSRLSLSL
                     EWLKKIHQILLPQGKKDQVQYRKDNPIHRMYFHDLSPADKISYRMRKLTDWFRTEECR
                     KAHPIELAVEVHRELIRIFPWPDISGRVARMAMNAILIGESYWPSIIHAIDRQTYYDV
                     LRLDQDQLLNLVVDGMEEGIRSSSRLYQSIVDASRG"
BASE COUNT          443 a          979 c          911 g          441 t
ORIGIN      
        1 cgccgcgtcg atctccgggt cgagcttgcc gaccccgcta aatacgatgc gagcccccgg
       61 gatgccggcc gcgagcgcgc gcgcgagctc gccgccggac acgatgtcgg ccccggcccc
      121 gcggcgcgcc agcgtgcgca gcaccgcctg gctgctgttg gccttgaccg cgtagcacag
      181 caggctcggg tgcccggcgc aggcgcgcgt cagctcgtcg aagcggccgc cgatctccgc
      241 gctgccgtag acgtaggtcg gcgtgccgac ggcggccgcg atgtcggcga gcgacacgcc
      301 ctcgacgcac aacaccccgc gcgtgacgcg gaggccgtgg cgctcggggg cggcgaacac
      361 cgcgacgtga tccggggcag tggggatggg ggcggctgcg accacgcaag ggaggtgcca
      421 cggccctgcg acgcctgtca aacgcgaccc ggctttgccg cgaggatcgg gcgctgctag
      481 gatcgcaggg tcacttcagg ccaccggctt taggaggtac ggatgaacca acaagcgaat
      541 cgctcgtgca ctttcacctt ccaacctcgc aggaccttcc acctcggagg tgccaggccc
      601 tagccggggc ctcgacgcga cgacggaccc cctcatcaag gactccgtcc gcgtcagggc
      661 tcgcagctga gacgccgctt cggtgaccgc cccggatggg gcacagatcg cgtcaccctt
      721 tccgggcctc cagagacgag aggccctccc gaggcaacaa cgccatgttg cctgcagatg
      781 tccccggtca ggttggcgcc tgaccacgcg tccgccacat gtcgtggcaa gggctgcctc
      841 acggcgcccc acccaaaact gggcctcgcg cccacaaccg aaccggctca gagctcgaag
      901 tgaccgcggc ggagcgcaat gttgcgctcc gccgcttgct tattcacggc gccccgcgcc
      961 tggacacctc gacggcgccc cgcgcctggg cactcgctcg gagacggctc ccgacagttc
     1021 tcaaaaagag ggccggctgc acgccccgct tcggttcaga gcaggtggcc gctcttgtcg
     1081 cgcttggtcg ccaggtagcc gacgttctgc tcgcgcgact cggcgatgat cgcctcgcga
     1141 cgatggacct cgacgccgcc ggcgcgcagg ccctcgatct tgcgcaggtt gttggtgtgc
     1201 agcaccaccg acaccacgcc gagcgcgtgc agcatcgcgg ccgcgaccga gtagtcgcgc
     1261 aggtcgtcgt cgaagccgag gtgcaggttg gcctcgaccg tgtcgagccc ggtctgctgc
     1321 agctggtacg cgcggacctt ctgcgcgatc ccgatgccgc ggccctcctg gcgcaggtac
     1381 aaaatgatgc cccgctcgcc ggtcgagatc cgggccatcg ccagctcgag ctggtcgcgg
     1441 cagtcgcagc ggtacgatcc gaacacatcg cccgtcaggc actccgagtg gatgcgggtc
     1501 gtcacctcgg cggcgccgtc gagctcgccg cggacgatcg cgacgtcgtc gagggtgcgg
     1561 cccgcctcgt cgatgaacga cacgatctcg aactcaccgt gtcgcgaggg cagccgcgcc
     1621 cgcgcgaaca cattgacgcc gcgcaggacc ttcggggctc ggacgctcag cgagtgcggg
     1681 actggcatcg gcgaaactcc gatcgaaggt tagcgcgtcc tggctagccg cgcgaggcgt
     1741 cgacgatgct ctggtacagc cgcgaggacg agcgaatccc ctcctccatc ccgtcgacga
     1801 cgaggttgag gagctggtcc tggtcgaggc gcagcacgtc gtagtaggtc tgacggtcga
     1861 tcgcgtggat gatcgacggc cagtagctct cgccgatcag gatcgcgttc atcgccatgc
     1921 gggcgacccg gccgctgatg tccggccagg ggaagatgcg gatcagctcg cggtggacct
     1981 cgacggccag ctcgatcggg tgggccttgc ggcactcctc cgtccggaac cagtcggtca
     2041 gcttgcgcat gcggtagctg atcttgtccg ccggcgagag gtcgtggaag tacatgcgat
     2101 ggatcgggtt gtccttgcgg tactggacct gatccttttt gccctggggc agaagaatct
     2161 gatggatctt cttgagccac tccagcgaca aagacagcct gctgccccgc gaatccagca
     2221 agaactggat cgcgtcgcgt acggccatga tgtgatagta ggcggggatc aagctcggcg
     2281 tggagatgat ctcgtcgtcg atcgccgcct tgatctcggc cacggacagc accgtgccct
     2341 cgagcgcgaa gtcgtgatag atccacgaca tgatgaagcg ctcgtcgaag ctgcgccgct
     2401 gcgccggcga cagcatgccg agcttctccc agagcaactc cgaagactcg aagatctggc
     2461 gatagttcct ggtccgcgct ggcgtcacgc tcgctccttc aaaacaacct cactgccgac
     2521 agggttctgc cttctcggcc aacccatgcc ggcccgcgct cgcgcggccg gtggccccct
     2581 cagggcacgc gaagctacgt cctcgccccc gccccggtca ataaaaagca tctaagcgcc
     2641 tgtttttccc ctgaaatttg acgcgcccgc gccgcttcgc taggccggct acccggtgtc
     2701 cgtcgcctac cacaagcccg tgctcgtcca ggaggtctgc gccgccctcg tgccccccct
     2761 gcgcgcggcg gtca
//
LOCUS       DASWNJ010000409         3607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_359685, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000409 DASWNJ010000000
VERSION     DASWNJ010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359685"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VGB85_07050"
     CDS             <1..471
                     /locus_tag="VGB85_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133819.1"
                     /translation="ELDSDIEDSLKAQVLATIVDLPEVVSLRAAFQADRASYDKETRA
                     ELPTWATYRDEHLVVTRWDEVGGGRSFVNVELGDGGEACSDMFAEQRFLLFALQGGRL
                     VQQPGVGFKRPLALMDLERDGSVEAVTLGGGRLGGAADDAVVQRFEIPYHGCEC"
     gene            complement(514..1869)
                     /locus_tag="VGB85_07055"
     CDS             complement(514..1869)
                     /locus_tag="VGB85_07055"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEY0133820.1"
                     /translation="MHHANRSTLVLVPLTAAMLVAPGCDEGAERTQFEDARVPVVMDH
                     EEMFGAYLPAPPAGPEVDHVDGASDLFTDAEEGTAIYGGTTVPVCGWPSTVELGGACT
                     GTLVHPQVVIYAAHCGASYSKIYFGENYQAPAKTATPSFCKVIPGGNPGSGNDFAVCK
                     LSTPVTNVPITPILMGCETSILQPGKAVTLVGFGNADNGPYGIKRHVTTTINGIDANN
                     EISIGGGGKDTCQGDSGGPAFVQLADGTWRVFGITSYGGECGTGGKYSMMHKGMSWFE
                     TTVGLDLTPCHNADGTWNPTAACGNFPKAPGVGGGTWSGGCSAGSPSGVLSSTCGAAY
                     GGGGQPPPPPPPATPCVGCTNYPGTLSGPGDSDQIPNGQYYQSLAAGVHEGYLVGPAN
                     SDFDLYLYKYINGAWSVVAKQENGGTTETIKYSGTAGYYTWLVSSYSGSGNYDFYLKK
                     P"
     gene            2319..3365
                     /locus_tag="VGB85_07060"
     CDS             2319..3365
                     /locus_tag="VGB85_07060"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0133821.1"
                     /translation="MSSETVLVTGAAGFIGHHVVRLLRAQGRQVRALLRPGEDARNLD
                     ELADPGVERVEGDILDRATLATHMAGCGVVYHLAAVYRTWVPDPKVIYDVNVTGTANV
                     LATAVALGVRRVVYTSSIAAIGLDPGGQPADEDTPYNLWPHAFDYVRSKYLAHHVALA
                     FAPILDLVVVNPGMPLGPGDIAPTPTGRTLIDTLNGKIRVSFEGGLNLVDVEDVARGH
                     LLAEARGRRGEAYLLTGHNLTIFEMVAELRRVVGMRHGMLRVPAGVASAVGRVLEGWA
                     ERVSRREPLITPGTVAYARQRLFYDHGKARDELGFTVRPLAETLHRAVDWFVAHGHVH
                     PGAAALGRDATPAA"
     gene            complement(3373..>3607)
                     /locus_tag="VGB85_07065"
     CDS             complement(3373..>3607)
                     /locus_tag="VGB85_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133822.1"
                     /translation="ALEAGGGMLRVAAELAPEDWTYEYMNDKNPIDHIYAATAATGGV
                     YIPGSARVFRSESGDDGYAGSDHAALRASFHAE"
BASE COUNT          522 a         1269 c         1262 g          554 t
ORIGIN      
        1 gagctcgact ccgacatcga ggacagcctc aaggcgcagg tgctcgccac gatcgtcgac
       61 ctgcccgagg tcgtcagcct gcgcgccgcc ttccaggccg accgcgcgag ctacgacaag
      121 gagacgcgcg ccgagctgcc cacctgggcg acctatcgcg acgagcacct cgtcgtgacg
      181 cgctgggacg aggtcggcgg cggccgcagc ttcgtcaacg tcgagctcgg cgacggcggc
      241 gaggcgtgca gcgacatgtt cgccgagcag cgcttcctgc tgttcgccct gcagggcggt
      301 cggctcgtgc agcagccggg tgtcggcttc aagcgcccgc tcgcgctgat ggacctcgag
      361 cgcgatggct cggtcgaggc ggtcacgctc gggggcgggc ggctcggcgg cgccgcggac
      421 gacgccgtcg tgcagcgctt cgagatcccg taccacggct gcgagtgttg aggcgccccg
      481 cggactgtcc gcggggccgt cgcatcgcgg cgctcagggc ttcttgaggt agaagtcgta
      541 gttgccgctg ccgctgtacg acgataccag ccaggtgtag tagccggcgg tgccgctgta
      601 cttgatcgtc tcggtggtgc cgccgttctc ctgcttggcg acgaccgacc acgcgccgtt
      661 gatgtacttg tagaggtaca ggtcgaagtc gctgttggcc ggcccgacca ggtagccctc
      721 gtgcaccccg gcggccaggc tctgatagta ctggccgttg gggatctggt cggagtcgcc
      781 cgggcccgac agcgtgccag ggtagttcgt gcagccgaca cacggcgtcg ccggcggcgg
      841 cggcggcggc ggctgaccgc caccaccata cgccgcgccg caggtgctgc tgagcacccc
      901 gctcggcgaa cccgcgctgc aaccaccgga ccaggtcccg ccgccgacgc ccggcgcctt
      961 cgggaagttg ccgcaggccg ccgtcgggtt ccaggtcccg tccgcgttgt gacacggcgt
     1021 caggtcgagg ccgaccgtgg tctcgaacca gctcatgccc ttgtgcatca tcgagtactt
     1081 gccgccggtg ccgcactcac ccccgtacga ggtgatgccg aacacgcgcc aggtgccgtc
     1141 ggcgagctgc acgaaggccg ggccgcccga gtcgccctga caggtgtcct tgccgccgcc
     1201 gccgatcgag atctcgttgt tggcgtcgat gccgttgatc gtggtggtga cgtggcgctt
     1261 gatcccgtag gggccgttgt ccgcgttgcc gaagccgacc agcgtgaccg ccttgccggg
     1321 ctgcaggatg ctcgtctcgc agcccatcag gatcggcgtg atcgggacgt tggtgaccgg
     1381 cgtcgacagc ttgcagaccg cgaagtcgtt gccgctgccc gggttgccgc cggggatgac
     1441 cttgcaaaag ctcggcgtgg cggtcttcgc gggcgcttga tagttctcgc cgaagtagat
     1501 cttgctatag ctggcgccgc agtgggccgc gtagatgacg acctgcgggt gcacgagcgt
     1561 gcccgtgcag gcgccgccga gctcgaccgt cgacggccag ccgcacaccg gcactgtcgt
     1621 gccgccgtag atcgccgtgc cctcctcggc gtcggtgaac aggtcgctgg ccccgtcgac
     1681 atggtcgacc tcgggcccgg caggcggcgc cggcaggtac gcgccgaaca tctcctcgtg
     1741 atccatcacg accgggacgc gggcgtcctc gaactgggtc cgctcggccc cttcatcaca
     1801 tccgggcgcg acgagcatgg cggcggtgag cgggaccaaa accagggtgc tgcgatttgc
     1861 gtggtgcatg tcgatcactt tcttgcgaag ggaacacggc tccgatcaat gtccacacct
     1921 ggcccaaagc gcaggtgtgt gcatatcggc cgggggtatg accgcggacg cgcgattttc
     1981 acgactcgcc gccgactgcg cctatcgagc cgagtacggc cgaataagcc agtgtccgtc
     2041 ctgcgagcgg gtcgcaggag tcgagcggcg gtccgaggag cggaaggggg aagcgagcac
     2101 atcctgcgta caatcagtac acaagttagt caacccaaaa accacacaaa cggcaacaac
     2161 agcacaaaca ccacaaatgt gccgacagcg ccgggggcga cgcgagacac actgcgctga
     2221 cctgggggcg ggacgccacc gagctcgctg taactcgctg tgactcgctg tgactcgctg
     2281 tatcagcacc gttcgccatc gcggctaagc tcgcggccat gtccagcgaa acagtcctcg
     2341 tcaccggcgc ggccggcttt atcggtcatc acgtggtccg gctgctgcgc gcgcagggtc
     2401 ggcaggtgcg ggcgctgctg cggcccggcg aggacgcccg caacctcgac gagctggccg
     2461 accccggcgt cgagcgcgtc gagggcgaca tcctcgaccg cgcgacgctg gcgacgcaca
     2521 tggccggctg cggcgtcgtc tatcacctcg cggcggtcta ccggacgtgg gtcccggacc
     2581 cgaaggtcat ctacgacgtc aacgtgacgg gcaccgccaa cgtgctggcg accgcggtgg
     2641 cgctcggggt ccggcgggtc gtgtacacca gctcgatcgc ggcgatcggc ctcgaccccg
     2701 gcgggcagcc ggccgacgag gacacgccgt acaacctctg gccgcacgcc ttcgactacg
     2761 tgcgctcgaa gtacctcgcg caccacgtcg cgctggcctt cgcgccgatc ctcgacctcg
     2821 tggtcgtcaa ccccggcatg ccgctcgggc ccggcgacat cgccccgacg ccgaccggtc
     2881 gcaccctgat cgacacgctc aacggcaaga tccgggtcag cttcgagggc ggcctcaacc
     2941 tcgtcgacgt cgaggacgtc gcgcgcgggc acctgctcgc cgaggcccgc gggcgccgcg
     3001 gcgaggccta cctgctgacc ggccacaacc tgacgatctt cgagatggtc gccgagctgc
     3061 ggcgggtcgt cggcatgcgc catgggatgt tacgcgtacc cgccggcgtc gcctccgcgg
     3121 tcgggcgcgt gctcgagggc tgggccgagc gcgtgtcccg acgcgagccg ctgatcaccc
     3181 cgggcacggt cgcgtacgcg cgccagcgcc tgttctacga ccacggcaag gcgcgcgacg
     3241 agctcggctt cacggtccgc ccgcttgccg agacgctgca ccgcgcggtc gactggttcg
     3301 tcgcccacgg ccacgtgcac ccgggggccg ccgcgctggg ccgggacgcg acgcctgcgg
     3361 cgtgagcccg gctcactcgg cgtggaagct cgcgcgcagg gccgcgtggt cggagccggc
     3421 gtagccgtcg tcgccgctct cgctgcggaa cacgcgggcc gagcccggga tgtagacccc
     3481 gccggtcgcc gcggtcgccg cgtagatgtg atcgatcggg ttcttgtcgt tcatgtactc
     3541 gtaggtccag tcctcgggcg ccagctcggc ggcgacgcgc agcatgccgc caccggcctc
     3601 gagcgcg
//
LOCUS       DASWNJ010000410         2314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_255467, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000410 DASWNJ010000000
VERSION     DASWNJ010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255467"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2271)
                     /locus_tag="VGB85_07070"
     CDS             complement(<1..2271)
                     /locus_tag="VGB85_07070"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY0133823.1"
                     /translation="MIFDSATKRFERKTLDQAIQMISIAPEGWYLVLKNPAADKSHPR
                     TGALNNLPNLDAGRKVNIPGPVSFALWPGQMMRVLQGHHLRSNQYLLVRVYDEEAAKL
                     NWKNAVIKPQKTGEGEAEVEGMLRLEASPDLTMGNLLVIRGTQVSFYIPPTGVEVVPD
                     VDGNYVREAVTLERLEYCILLDENGSKRFIVGPDVVFPKPTEVIVRMRGSRKFRAIEL
                     NENSGLYLKVIAPYEENGVSHKVGDEIFLTGRDQMIYFPRTEHAIVKYGDQEKHHAVA
                     IPAGEARYVLDRLTGKISLKRGPAMFLPDPRREVIVRRVLDPKTVALWFPGNREAYEY
                     NRRLAAQNAPQVAAEYGSSAAPTPALASRAAAPASKPAAGASGAIGTREAQAAPPPPN
                     EFAGDDFNRNQTFTGPRTIVLDTRFEGAVAIDVWTGYAVLVVSKTGERQVVSGPHTRL
                     LEYDENLQVLELSTGTPKSDQNLFKTVYLRTLNNKVTDVVEAETQDLVRVHLQLSYRV
                     NFEGDAARWFEVENYVKFLTDHLRSLIRHAVKRHGIEKFYADSVAILRDIILGTVGED
                     GKRPGRRFEENGMRIYDVEVLEVAIGDDTIAGLLVKAQHAAVQQTLQLAAEQRRLEAT
                     RQSESISQQIAEVQSQTRQKASGLKTLEVEQQLRHQLAEQAAKIELHERQQTALLTEQ
                     ERIGAIHQADLARRRAVGEFELAIAQGKLDQKLRELAAEVTAVAEKARAVSPDLIAAL
                     QAFGDRALAEKMAESMA"
BASE COUNT          307 a          834 c          760 g          413 t
ORIGIN      
        1 ggccatgctc tcggccatct tctcggccag cgcccggtcg ccgaacgcct gcagcgcggc
       61 gatcaggtcc ggcgagaccg cccgcgcctt ctcggccacc gcggtgacct ccgcggcgag
      121 ctcgcgcagc ttctggtcga gcttgccctg cgcgatcgcc agctcgaact cgccgaccgc
      181 cctgcgccgg gccaggtccg cctgatggat cgcgccgatg cgctcctgct cggtcaacag
      241 cgcggtctgc tggcgctcgt gcagctcgat cttcgccgcc tgctcggcga gctggtggcg
      301 cagctgctgc tcgacctcca gcgtcttgag cccgctcgcc ttttgccgcg tctgcgactg
      361 cacctccgcg atctgctgcg agatcgactc gctctggcgc gtcgcctcga gtcggcgctg
      421 ctccgccgcc agctgcagcg tctgctgaac cgccgcgtgc tgagccttga ccagcaggcc
      481 ggcgatcgtg tcatcgccga tcgcgacctc gagcacctcg acgtcgtaga tccgcatgcc
      541 gttctcctcg aagcggcgac ccggacgctt gccgtcctcg ccgaccgtgc cgaggatgat
      601 gtcacgcagg atcgccaccg agtcggcgta gaacttctcg atcccgtggc gcttgaccgc
      661 gtggcggatc agcgagcgca ggtggtcggt caggaacttg acgtaattct cgacctcgaa
      721 ccagcgcgcc gcgtcgccct cgaagttgac gcggtacgac agctgcaagt gcacgcgcac
      781 caggtcctgc gtctccgcct cgacgacgtc cgtcaccttg ttgttgaggg tgcgcaggta
      841 caccgtcttg aacaggttct ggtccgactt cggcgtgccg gtcgagagct cgagcacctg
      901 caggttctcg tcgtactcga gcaggcgcgt gtgcggcccg ctgacgacct ggcgctcgcc
      961 ggtcttgctg acgacgagca ccgcgtagcc ggtccacacg tcgatcgcca ccgcgccctc
     1021 gaagcgcgtg tcgagcacga tcgtgcgcgg cccggtgaag gtctggttgc ggttgaagtc
     1081 gtcgcccgcg aactcgttcg gcggcggcgg tgcggcctgg gcctcgcgcg tgccgatcgc
     1141 gcccgaggcc ccggcggcgg gcttgctcgc cggcgcggcc gcccgcgacg cgagggccgg
     1201 cgtcggcgcg gcggacgatc cgtactcggc ggccacctgc ggggcgttct gggccgcgag
     1261 ccggcggttg tactcgtagg cctcgcggtt gcccgggaac cacagcgcga ccgtcttcgg
     1321 gtcgagcacg cggcgcacga tcacctcgcg ccgcgggtcg ggcaggaaca tcgccggccc
     1381 gcgcttgagt gaaattttgc cggtcaggcg gtcgagcacg tagcgcgcct cgccggccgg
     1441 gatcgcgacc gcgtggtgct tctcctgatc gccgtacttg acgatcgcgt gctccgtgcg
     1501 cgggaagtag atcatctgat cgcggccggt caggaagatc tcgtcgccga ccttgtgcga
     1561 gaccccgttc tcctcgtacg gcgcgatcac cttgaggtac agcccggagt tctcgttgag
     1621 ctcgatcgcc cggaacttcc gcgagccccg catgcgcacg atcacctcgg tcggcttcgg
     1681 gaacacgacg tccggcccga cgatgaaccg cttcgacccg ttctcgtcga gcaagatgca
     1741 atactcgagg cgctcgagcg tcaccgcctc gcgcacgtaa ttgccgtcga cgtcgggcac
     1801 cacctcgacg ccggtcggcg ggatgtagaa cgacacctgc gtcccgcgga tcaccagcag
     1861 gttgcccatc gtcaggtccg gcgaggcctc gaggcgcagc atgccctcga cctccgcctc
     1921 gccctcgccg gtcttctgcg gcttgatcac cgcgttcttc cagttgagct tggccgcctc
     1981 ctcgtcgtac acgcggacca gcaggtattg gttgctgcgc aggtgatgcc cctgcaacac
     2041 ccgcatcatc tgccccggcc acagcgcgaa cgacaccggc cccgggatgt tgaccttgcg
     2101 gcccgcgtcg aggttcggga ggttgttgag cgccccggtc cgcggatggc tcttgtccgc
     2161 cgccgggttc ttgagcacca gataccagcc ctccggcgcg atcgagatca tctggatcgc
     2221 ctggtcgagc gtcttgcgct cgaaccgctt ggtcgcagag tcgaagatca ccggcacatc
     2281 cgtgttggcc aggctcgtct tgtgcggccc gacg
//
LOCUS       DASWNJ010000411         2738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_148442, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000411 DASWNJ010000000
VERSION     DASWNJ010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148442"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VGB85_07075"
     CDS             complement(<1..478)
                     /locus_tag="VGB85_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133824.1"
                     /translation="MTTETQIFSAVWSVIRRRALDRGVAATELCDQVIRSHGKLHRVE
                     GKLRAGTTILDENNVILPMLARATSLGFSLYLGNRRVSSVSVLGAGKPGDIGAYADAE
                     LVDNVLRKREVWKGTLQRNGNTILSMARPLYLGDGPDEAGPIGMIEAVLNEQAFFES"
     gene            604..2046
                     /gene="gltX"
                     /locus_tag="VGB85_07080"
     CDS             604..2046
                     /gene="gltX"
                     /locus_tag="VGB85_07080"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEY0133825.1"
                     /translation="MRIAPSPTGDPHVGTAYIALFNYVFARKTGGTFILRIEDTDQER
                     YTAGSEAAIFEALRWLGLGWDEGPDVGGPYGPYRQSERLTIYQSHVGELLARGEAYRC
                     FCDRERLEALRKQQEAAKASKLGYDGHCRHLSEEDVANKLAGGVPFVVRLRVPDEGAT
                     EFVDHLRAEPIRFDNRLVDDQVLQKSDGFPTYHLANVVDDHLMGITHVIRGEEWITST
                     PKHVLLYRAFGWEPPQFYHLGLLRNADKSKLSKRKNPVSIFHYRDLGYLPETLLNFMA
                     NLGFSIGPEVERFTIEQMIEHFDWSKVSVGGPVFDPAKLDAFNGADIRAMSVDALYDR
                     LLERTLAPARMKGLLAQAQERVNRLDDFIPYLSFFFGGSLDYAPVKDKIKPIAGKTGA
                     ESAQVLDEFVQEIEKDNQARSFTAAALEAFARDFCQRHAVKPKELFMLLRLAVTGRTA
                     SPSLFDTLALVGKDRARTRIKDIGELLRKM"
     gene            complement(2142..2315)
                     /gene="ssrS"
                     /locus_tag="VGB85_07085"
     ncRNA           complement(2142..2315)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VGB85_07085"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(2426..>2738)
                     /locus_tag="VGB85_07090"
     CDS             complement(2426..>2738)
                     /locus_tag="VGB85_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133826.1"
                     /translation="STGPHGGNVRTFVNDLALASLAAQNVEHPQDSATVKELYGDGVD
                     TVIGYAVSRKTAPASQGGATWYWYERINATTYGGDLGVGLCTGCHGGGADFILTPFPL
                     Q"
BASE COUNT          463 a          948 c          900 g          427 t
ORIGIN      
        1 gcgactcgaa gaacgcctgc tcgttgagga cagcctcgat catgccgatc gggccggcct
       61 cgtcggggcc gtcgccgagg tacagcgggc gggccatgga gaggatggtg ttgccgttgc
      121 gctgcagcgt gcccttccag acctcgcgct tgcgcagcac gttgtcgacc agctcggcgt
      181 cggcgtaggc cccgatgtcg cccggcttgc cggccccgag cacgctgacc gacgagacgc
      241 ggcgattgcc gaggtagagc gagaagccca gcgaggtcgc gcgcgcgagc atcggcaaga
      301 tcacgttgtt ctcgtcgagg atcgtggtgc cggcgcgcag cttgccctcg acccgatgga
      361 gcttgccatg gctccggatc acctggtcgc acagctcggt ggccgcgacg ccacggtcga
      421 gggcacgccg ccggatcacc gaccacactg cgctgaagat ctgggtctcg gtcgtcatat
      481 cactcacgcg ccggcagtgt aacggccctt tgggccgcgt ggtaggcagg ggccatgcgc
      541 gaggcacaag acgcggtttc gacccccggt ggctcccacg ggcactccgg agcccctccg
      601 agggtgcgga tcgccccgag cccgaccggc gacccgcacg tcgggaccgc ctacatcgcc
      661 ctgttcaact acgtgttcgc ccgcaagacc ggcggcacct tcatcctgcg gatcgaggac
      721 acggaccagg agcgctacac ggccggcagc gaggcggcga tcttcgaggc cctgcgctgg
      781 ctcgggctcg gctgggacga gggcccggac gtcggcggcc cgtacggccc gtaccgccag
      841 tcggagcgcc tgacgatcta tcagagccac gtcggcgagc tgctggcccg cggcgaggcc
      901 taccgctgct tctgcgaccg cgagcgcctg gaggcacttc gcaagcaaca ggaggcggcc
      961 aaggcgtcca agctcggtta cgacggccac tgccgccacc tgagcgagga ggacgtcgcc
     1021 aacaagctgg ccggcggggt gcccttcgtc gtccgcctgc gtgtgccgga cgagggcgcg
     1081 acggagttcg tcgaccacct gcgcgccgag ccgatccgct tcgacaaccg cctcgtcgac
     1141 gaccaggtgc tgcaaaagtc ggacggcttc ccgacctacc acctcgccaa cgtcgtcgac
     1201 gaccacctga tgggcatcac ccacgtgatc cgcggcgagg agtggatcac ctcgacgccc
     1261 aagcacgtgc tgctgtaccg cgcgttcggc tgggagccgc cgcagttcta ccacctgggt
     1321 ctgttgcgca acgccgacaa gtccaagctg tcgaagcgca agaacccggt ctcgatcttc
     1381 cactaccgcg acctcggcta tctcccggag acgctgctca acttcatggc gaacctcggc
     1441 tttagcatcg ggcccgaggt cgagcgcttc acgatcgagc agatgatcga gcacttcgac
     1501 tggtccaagg tcagcgtcgg cggcccggtg ttcgacccgg ccaagctcga cgcgttcaac
     1561 ggcgccgaca tccgggcgat gtcggtcgac gcgttgtacg accgcctgct cgagcggacc
     1621 ctcgcccccg cgcgcatgaa ggggctgctc gcccaggcgc aggagcgggt caaccgcctc
     1681 gacgacttca tcccgtacct gagcttcttc ttcggcggca gcctcgacta cgcgccggtc
     1741 aaggacaaga tcaagccgat cgccggcaag accggcgccg agtcggccca ggtgctcgac
     1801 gaattcgtgc aggagatcga aaaggacaac caggcccgca gcttcaccgc cgcggcgctc
     1861 gaggccttcg cccgcgactt ctgccagcgc cacgccgtca agcccaagga gctgttcatg
     1921 ctcctgcgcc tcgccgtcac cggacgcacc gcgtcgccct cgctgttcga cacgctcgcg
     1981 ctcgtcggca aggaccgcgc ccgcacccgc atcaaggaca tcggcgagct gctgcgcaag
     2041 atgtgaggcc ggaaaagcga acgggtgagg gtccgtggga cgctcacccg tggtgctgat
     2101 ggcccatcac gagacgagca ggcctttcag cgaaaattaa agagcgggtt gctcggcgtc
     2161 tgaccagaaa gttcaacccc tacatatgtg taggtgggga ctatggtacc cgcttcgggc
     2221 ttctcgtcga acgagcctgc acacctgcgg acagagacgg acccctggct gaacgatgta
     2281 ggtagaactt gtggcctccg acagagcaac ccgctcgtcg ccaaccatag caactctttt
     2341 ccgggatgga agtccccaag ttcgcgccgc gtcacacgcc ctgtgataac cacggtcgcg
     2401 tggtgatcac ggcggcgcgc gctgctcact gcaacgggaa cggcgtgagg atgaagtccg
     2461 caccgccgcc gtgacagccg gtacaaagtc cgacgcccag gtcaccgccg taggtggtag
     2521 cgttgatcct ctcgtaccag taccaggtgg cgccgccctg gctcgcgggt gccgtcttgc
     2581 gcgagacggc gtagccgatg accgtgtcga cgccgtcgcc gtacagctcc ttgaccgtcg
     2641 cgctgtcctg cggatgttcg acgttctggg ccgcgagcga ggccagcgcg aggtcgttga
     2701 cgaaggtgcg gacgttgccg ccgtgcgggc ccgtggag
//
LOCUS       DASWNJ010000412         2750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_322987, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000412 DASWNJ010000000
VERSION     DASWNJ010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322987"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VGB85_07095"
     CDS             complement(<1..369)
                     /locus_tag="VGB85_07095"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="catalyzes the conversion of guanosine
                     5'-triphosphate,3'-diphosphate (pppGpp) to guanosine
                     5'-diphosphate,3'-diphosphate (ppGpp); pppGpp and ppGpp
                     control the stringent response during amino acid
                     starvation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     pyrophosphatase"
                     /protein_id="HEY0133827.1"
                     /translation="MSNSESVAAVDLGSNSFHMVVARVVDDTHFHIVDRLRERVALAA
                     GLDEQYQLSDKAQARAVACLERFGQRLREIPLDRVRAVGTSALRQATNTRAFLPAAQA
                     ALGHPIEIIAGSEEARLIYLG"
     gene            complement(428..838)
                     /locus_tag="VGB85_07100"
     CDS             complement(428..838)
                     /locus_tag="VGB85_07100"
                     /inference="COORDINATES: protein motif:HMM:NF040111.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amphi-Trp domain-containing protein"
                     /protein_id="HEY0133828.1"
                     /translation="MKFARNDLKVEGAASVAHVAAYLEQLAQALRAGTAHVRQGDQQI
                     VLGPSDVVGFSLAASDRGKRQSVSLELTWRKRNIPATELDLHFGSEAPATESTEATEV
                     GTSPGLEDEASVLTGVPGTDTDASKGEAHEGEKL"
     gene            1047..>2750
                     /locus_tag="VGB85_07105"
     CDS             1047..>2750
                     /locus_tag="VGB85_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133829.1"
                     /translation="MSASDRSQTDLEVLSSSHRGKFITLGVMLAAAGGFWWFMNREGP
                     IGNDEDPSRVLVVSASHIRYKPYLEQWGFIAQEGQAQTIEEEAKRQLPDMKETGVAAV
                     LKLADKTGYAYVAFEHPAQVDFSGLEIEGGLPTFEPHHRFAVLSAGDYAYPHKLTVNP
                     KPSEVMRGVDLDLLSALFAQEPLASTLRDDPKNPPEVMLLRGTLQEGMHRLEAIKDAE
                     RTVAKIADKARALLVDKEQADPKPAMLGGIHESLNGVALADGSTLVLGRQVHFSSTGY
                     KAELELDRNWEFSYLPAGSASFAAPGAARGSERTPCPTLMGGTLEETGSRPIFRFSPR
                     GDAVLVHTEGVSQVWKLDHGDGKPACNFVLAGKVGVPLARGEDPGEPHRSGMVARGRF
                     DGDDSVVYVVKPNEEVPTELVRSPSIRFDPPAWIADEWIAAAGYATGAETGGMYLMSP
                     AHPDHALRLDAMALDASTAVHQIAPAPDGPNGPRLLVTASSERGQRLFRVDVARPWNQ
                     LFTDTLAADKTPPPQSVVDGTEPRILHLDGAGFTATALTNEGWVSDMVAAPDGSAAGF
                     QI"
BASE COUNT          404 a         1024 c          940 g          382 t
ORIGIN      
        1 gccgaggtag atcaggcgcg cctcctcgct cccggcgatg atctcgatcg ggtggccgag
       61 cgccgcctgg gccgccggca aaaacgcccg cgtgttggtc gcctggcgca gcgcgctggt
      121 cccgaccgcg cgcacccggt cgagcgggat ctcgcgcagg cgctggccga agcgctcgag
      181 gcaggccacc gcgcgcgcct gcgccttgtc gctcagctgg tactgctcgt cgagcccggc
      241 cgccagcgcc acccgctcgc gcagacggtc gacgatgtgg aagtgcgtgt cgtcgacgac
      301 ccgcgccacc accatgtgga agctgttcga cccgaggtcg accgccgcga cgctctcgct
      361 gttcgacacc gggcgcgact atacgggcca acgaccgcgc gccgcaagcg gcgccgcccg
      421 cccgcgtcta cagcttctcg ccctcgtgcg cctcgccctt gctggcgtcg gtgtcggtgc
      481 ccgggacccc cgtcagcacg gacgcctcgt cctcgagccc ggggctcgtg ccgacctcgg
      541 tcgcctcggt cgactcagtc gcgggcgcct cgctgccgaa gtgcaggtcg agctcggtgg
      601 cggggatgtt gcgctttcgc caggtcagct cgagcgagac gctctgccgc ttgccacggt
      661 cactggcggc gagcgagaag ccgacgacat cgctgggccc gagcacgatc tgctggtcac
      721 cctgacggac gtgggcggtg cccgcccgga gggcctgggc cagctgctcg agatacgcgg
      781 ccacgtgcgc caccgaagcg gcgccctcca ccttgagatc gttgcgtgca aacttcatga
      841 gacggctccg ggcccggcgg cccggacgca tggtgacacg ggtgggaccc gaccgacagc
      901 cttccccacc ggatgcccgg ccgctcggca tttcatgcgg gcgggccgcg cggccccgcc
      961 tcgctcgccg gccccgcctg caaaagccca cgaggggctc ccggcggcct gtcccgggtc
     1021 gcgcattttg tgccaaagta gcgaccatgt ccgcctccga ccgctcccag accgacctcg
     1081 aggtgctcag cagcagccat cgcggcaagt tcatcaccct cggcgtcatg ctcgcggcgg
     1141 ccggcgggtt ctggtggttc atgaaccgcg aggggccgat cgggaacgac gaggacccga
     1201 gccgcgtgct cgtcgtcagc gccagtcaca ttcgttacaa gccgtacctc gagcagtggg
     1261 gcttcatcgc gcaggagggc caggcgcaga cgatcgaaga ggaggcgaag aggcagctgc
     1321 ccgacatgaa ggagaccggc gtcgcggccg tgctcaagct cgccgacaag accggctatg
     1381 catatgtcgc cttcgagcac ccggcccagg tcgacttcag cggcctcgag atcgaaggcg
     1441 gcctgccgac cttcgagccg catcaccgct tcgcggtcct cagcgccggt gattatgcat
     1501 atccccacaa gctgaccgtc aaccccaagc cctcggaggt gatgcgcggc gtcgacctcg
     1561 acctgctgtc cgcgctgttc gcccaggagc cgctggcgag cacgctgcgc gacgacccca
     1621 agaacccgcc ggaggtgatg ctgctacgcg gcaccttgca ggagggcatg caccgcctcg
     1681 aggcgatcaa ggacgccgag agaaccgtcg ccaagatcgc cgacaaggcg cgcgcgctgc
     1741 tcgtcgacaa ggagcaggcc gaccccaagc cggcgatgct cggggggatc cacgagagcc
     1801 tcaacggcgt cgccctcgcg gacggcagca cgctcgtgct cgggcgccag gtccacttct
     1861 cgagcaccgg ctacaaggcg gagctcgagc tcgaccgcaa ctgggagttc tcgtacttgc
     1921 ccgccggcag cgcttctttc gcagcgcccg gcgccgctcg cggctcggag cgcacgccct
     1981 gtccgacgct gatgggcggc acgctcgagg agaccggcag ccgaccgatc ttccgcttct
     2041 cgccccgggg cgacgccgtg ctggtccaca ccgagggtgt gtcgcaggtg tggaagctcg
     2101 accatggcga cggcaagccg gcgtgtaact tcgtgctcgc cggcaaggtc ggcgtgccgc
     2161 tggcgcgcgg cgaggacccg ggtgagccgc accgcagcgg catggtcgcg cgcggccgct
     2221 tcgacggcga cgactcggtg gtctacgtgg tcaagccgaa cgaggaggtc ccgaccgagc
     2281 tggtgcgcag cccgtcgatc cgcttcgacc cgcccgcgtg gatcgccgac gagtggatcg
     2341 ccgccgccgg ttatgcgacg ggcgccgaga ccggcggcat gtacctgatg agccccgcgc
     2401 accccgacca cgcgctgcgc ctcgacgcga tggccctcga cgcctcgacc gccgtgcatc
     2461 agatcgcgcc cgcgccggac ggccccaacg gcccgcgctt gctcgtcacc gcctcgagcg
     2521 agcgtggcca gcggctgttc cgcgtcgatg tcgccaggcc ctggaaccag ctgttcaccg
     2581 acacccttgc cgccgacaag acgccgccgc cgcagtccgt ggtcgacggc accgagccgc
     2641 ggatcctcca cctcgacggc gccggcttca cggcgacggc gctgaccaac gagggctggg
     2701 tctccgacat ggtcgccgcg ccggacggca gcgccgcggg gttccagatc
//
LOCUS       DASWNJ010000413         2343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_466847, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000413 DASWNJ010000000
VERSION     DASWNJ010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466847"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="VGB85_07110"
     CDS             <1..818
                     /locus_tag="VGB85_07110"
                     /inference="COORDINATES: protein motif:HMM:NF036933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HEY0133830.1"
                     /translation="GGKVQAGAGVAADGTGQVTLGADDVLQVATAAGGDLTGTHIQAT
                     GPIQVIAGHKCAKVTPDIDLCDHLEEAMFPVEALAKEYIVVPPLQSPVDMNKSEQGQV
                     VRVIASEPDTTLTFTPDQGVPTKLAKAGDFVEIVKTKARFMITGDKKILVAQYMMGQG
                     AGFGEQDPSMLLAVATEQYRKDYLFQAPNSWEANFVDIILKSGTKVTVDGKAIAGFIK
                     IDGTEYSLAHVPLAKDGTGAHTLTASDRVGISVYGIQDYGSYWLVGGLDLDHL"
     gene            1136..>2343
                     /locus_tag="VGB85_07115"
     CDS             1136..>2343
                     /locus_tag="VGB85_07115"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0133831.1"
                     /translation="MFRPALGAVPANTPELVGTDARLAAIEAELLSGLRGAAEIVAVH
                     RVPARNGELVDWPVAIDPRLLASLATRGIERPYAHQATALAAALSGRDVVLATATASG
                     KSLCFQVPIVQSVLADPLSRALMLFPTKALARDQVRSLRELAVAGGGDGLLGCGAYDG
                     DTPPDERRAARAKAHAVATNPDMLHRGILPHHDRWSELLANLRYVVIDELHSYRGVFG
                     SHVANVLRRLWRVCAYHGSRPTVICCSATIANPRELASALTGRPDFTLIDRDTAPAGR
                     RTFVVLNPAVVDPLTGVRRDYLKVTRAATGAFRRGKVATLAFCRTRKAVELLTRYLRE
                     DEAGIEPGQAGGVDPSAVARAEQRIRGYRGGYLPEHRREVEQALHSGAAQVVISTHAL
                     ELGMDIGGLDA"
BASE COUNT          347 a          816 c          823 g          357 t
ORIGIN      
        1 ccggcggcaa ggtgcaggcg ggggccggcg tcgcggccga cggcaccggc caggtgacgc
       61 tcggcgccga cgacgtcttg caggtcgcga ccgcggccgg cggcgacctg acgggcacgc
      121 acatccaggc gaccggaccg atccaggtga tcgccggcca caagtgcgcc aaggtgacgc
      181 cggacatcga cctctgcgac cacctcgagg aggcgatgtt cccggtcgag gcgctggcga
      241 aggagtacat cgtcgtgccg ccgctgcagt cgccggtcga catgaacaag tcggagcagg
      301 gccaggtcgt ccgcgtgatc gccagcgagc cggacacgac gctgaccttc acgccggatc
      361 agggcgtgcc gaccaagctc gccaaggccg gtgacttcgt cgagatcgtc aagaccaagg
      421 cgcgcttcat gatcaccggc gacaagaaga tcctcgtcgc gcagtacatg atggggcagg
      481 gggccggctt cggtgagcag gacccgtcga tgctgctggc ggtcgccacc gagcagtatc
      541 gcaaggacta cctgttccag gccccgaact cgtgggaggc caacttcgtc gacatcatcc
      601 tcaagagcgg caccaaggtg acggtcgacg gcaaggcgat cgccggcttt atcaagatcg
      661 acggcaccga gtactcgctg gcccatgtgc cgctggccaa ggacggcacc ggggcgcaca
      721 cgctgaccgc cagcgatcgc gtcgggatca gcgtctacgg gatccaggac tacggcagct
      781 actggctggt cggcggcctc gacctcgacc acctgtaggg cctccccggg gcctgttcag
      841 agccgctgca ggccccgagg gtctgcgcag gatgcgcaaa acccggatcg agatctcatc
      901 ttcgatccgg gtttttcatg tcgcgtccgt cgaagtgccc gtggatgccg ctgcaacgat
      961 cttgacgcgc gttgctaaac gggtgtacct gcgttccaag tgttcagtgt gaacgtctcc
     1021 gggcaacgat gctagagcac tcttcaggct ttccccgcgc gcgtccctcc ttcaccccga
     1081 ggcagatccc ggcaccacgt gcaggtacct gcctcacccg ccgcggggga gcctgatgtt
     1141 tcgtcctgcc ctcggtgccg tcccggcgaa cacgccggag ctggtcggca ccgacgcgcg
     1201 gctcgccgcg atcgaggccg agctgctctc gggcctgcgc ggcgcggcgg agatcgtcgc
     1261 cgtacaccgg gttccggcgc gtaacggcga gctcgtcgac tggccggtgg cgatcgaccc
     1321 gcgcctgctc gcgtcgctcg cgacgcgggg gatcgagcga ccctacgccc accaggcgac
     1381 cgccctcgcg gccgcgctct cgggtcggga tgtcgtgctg gcgacggcga cggcgtccgg
     1441 taaatcgctt tgctttcagg tcccgatcgt gcagtcggtg ctcgcggatc ctctctcccg
     1501 ggcgttgatg ctgttcccga ccaaggcgct ggcgcgcgac caggtccgca gcctgcgcga
     1561 gctggcggtc gccggtggtg gtgacggcct gctcggctgc ggcgcctacg acggcgacac
     1621 gccgccggac gagcgccggg ccgcgcgcgc caaggcccac gcggtcgcga ccaacccgga
     1681 catgctgcac cgcgggatct tgccgcatca cgaccgctgg tcggagctgc tggccaacct
     1741 gcgctacgtc gtcatcgacg agctgcacag ctaccgcggc gtgttcggca gccacgtcgc
     1801 caatgtgctc cgccgactgt ggcgggtctg cgcgtaccac ggctcgcggc cgaccgtgat
     1861 ctgttgctcg gcgacgatcg ccaacccgcg cgagctggcg agcgccttga ccggtcgccc
     1921 ggacttcacg ctgatcgatc gcgacaccgc gccggccggg cgccgcacct tcgtggtgtt
     1981 gaaccccgcg gtcgtcgacc cgttgacggg ggtgcgccgc gattatctca aggtcacgcg
     2041 cgcggcgacc ggggcgtttc gccgcggcaa ggtcgcgacg ctggcgtttt gtcgcacgcg
     2101 caaggcggtc gagctgctga ccaggtacct gcgcgaggac gaggccggca tcgagccggg
     2161 ccaggccggg ggcgtcgatc cgtcggcggt ggcgcgcgcc gagcagcgga tccgtggtta
     2221 ccgcggcggc tatctgccgg agcaccgccg cgaggtcgag caggcgctgc actccggcgc
     2281 ggcgcaggtc gtgatctcga cgcacgcgct cgagctcggc atggacatcg gcgggctcga
     2341 tgc
//
LOCUS       DASWNJ010000414         2115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_359894, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000414 DASWNJ010000000
VERSION     DASWNJ010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359894"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VGB85_07120"
     CDS             <1..385
                     /locus_tag="VGB85_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0133832.1"
                     /translation="ENVELPLTYRNLSAAERRRRVDAALTRVGMANRARHLPAQLSGG
                     QQQRVAVARAVAGEPLLLLADEPTGNLDSKNGDAVMDLLHEMHGAGTTIVMVTHNTEY
                     ARKAQRTIQLFDGRVVEESTHGRTA"
     gene            369..>2115
                     /locus_tag="VGB85_07125"
     CDS             369..>2115
                     /locus_tag="VGB85_07125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0133833.1"
                     /translation="MGALLRDLRFGVRMLAKSPGYTLVSIMTLALAIGANTAIFSTIN
                     AVLLRPLPYADPERLIVLTETQPKFEEMSVAYPNFVDYRHKNATFVDMGAFRLDSLNL
                     TGKGEPERLKARSVSHSVLPVLGVEPMLGRNFLPEEDAPRGRKAVILTHGFWQRRFAG
                     DPSIVDRSITMSGDEWTVVGVMPPEFRFYGTSDILIPLGIRADDPNFKSRGSHPGLNV
                     VGRLKPGVTIAQARADLRGVGDAIAEADAQNYGTTRPRIQDLHEDIAEDYRSGLLLLF
                     GAVLFVLLIAAANVANLMLARAMARQKEMRIRAALGSGRWRLVRQLLVESSLLGLAGG
                     ALGLLIALWGVDLFAAARPQAIELLGPIGIDLPVLAYTLVIAVGTGLVFGLVPAIYAS
                     RQDLSQALKDADHHATAGGGHLRIRNMLVIAEVALALVLLVGAGLSIRGFMRTQMIDT
                     GFVPENVLTMEVALPSTTYKTAEQVRRFWTEVERRVAAIPGVETVSATAGLPFAGASE
                     SSFTPMGEPRGVDNVRMAVAYFVDAGYLETLKIPLLAGRTFGAQDTHETPPGLIIDQA
                     FAEKFFPGQDPVGQHV"
BASE COUNT          316 a          757 c          740 g          302 t
ORIGIN      
        1 cgagaacgtc gagctgccgc tgacctaccg caacctgtcg gcggccgagc ggcggcgccg
       61 ggtcgacgcc gcgctgacgc gcgtcggcat ggccaaccgc gcgcggcacc tgccggccca
      121 gctctcgggc ggtcaacagc agcgcgtcgc ggtcgcgcgg gcggtcgcgg gcgagccgct
      181 gctgctgctc gccgacgagc cgaccggcaa cctcgacagc aagaacggcg acgccgtgat
      241 ggacctgctg cacgagatgc acggcgccgg cacgacgatc gtcatggtga cacacaacac
      301 cgagtacgcg cgcaaggccc agcgcacgat ccagctgttc gacggccgcg tcgtcgagga
      361 gagcacccat gggcgcactg cttagagacc tgcgcttcgg cgtccggatg ctcgccaaga
      421 gccccggata taccctcgtg tcgatcatga cgctcgcgct ggcgatcggc gccaacacgg
      481 cgatcttcag cacgatcaac gccgtgctgc tgcgcccgct gccctacgcg gacccggagc
      541 ggctgatcgt cctcaccgag acgcagccca agttcgagga gatgtcggtc gcgtacccga
      601 acttcgtcga ctaccggcac aagaacgcga ccttcgtcga catgggcgcg tttcgcctcg
      661 acagcctcaa cctgaccggc aaaggcgagc cggagcggct caaggcgcgc agcgtctcgc
      721 actcggtgct gccggtgctc ggcgtggagc cgatgctcgg gcgcaacttc ctcccggagg
      781 aggacgcccc gcgcgggcgc aaggccgtga tcttgaccca cggcttctgg cagcgccgct
      841 tcgccgggga cccgtcgatc gtcgacaggt cgatcacgat gagcggcgac gagtggacgg
      901 tcgtcggggt catgccgccg gagtttcgct tctacggcac gagcgacatc ctcatcccgc
      961 tggggatccg cgccgacgac ccgaacttca agtcgcgcgg cagccacccg ggcctcaacg
     1021 tcgtcgggcg gctcaagccc ggcgtgacga tcgcccaggc gcgcgccgac ctgcgcgggg
     1081 tcggcgacgc gatcgcggag gccgacgcgc agaactacgg gaccacgcgc ccgcggatcc
     1141 aggacctgca cgaggacatc gccgaggact accgcagcgg cctgctgctg ctgttcggcg
     1201 cggtactctt cgtgctgctg atcgccgcgg ccaacgtcgc caacctgatg ctcgcgcggg
     1261 cgatggcgcg gcaaaaagag atgcggatcc gggccgcgct cggctcggga cgctggcggc
     1321 tggtccgtca gctgctggtc gagagctcgc tgctcgggct cgccggcggg gcgctcggcc
     1381 tgttgatagc actctggggc gtcgacctgt tcgcggccgc gcggccgcag gcgatcgagc
     1441 tgctcggccc gatcggcatc gacctgccgg tgctcgccta caccctcgtg atcgcggtcg
     1501 gcaccggcct ggtgttcggg ctcgtgcctg cgatctacgc gtcgcgccag gacctgtcgc
     1561 aggcgctcaa ggacgccgac caccacgcga ccgccggcgg cggacacctg cggatccgca
     1621 acatgctggt catcgccgag gtcgcgctcg cgctggtgct gctggtcggc gccggcctgt
     1681 cgatccgcgg cttcatgcgc acgcagatga tcgacaccgg cttcgtgccg gagaacgtgc
     1741 tgacgatgga ggtcgcgctg ccgagcacga cctacaagac ggccgagcag gtgcgtcggt
     1801 tctggaccga ggtcgagcgg cgcgtcgcgg cgatcccggg cgtcgagacc gtgtcggcca
     1861 ccgccggcct gccgttcgcg ggcgcgtcgg agagctcgtt cacgccgatg ggcgagccgc
     1921 gcggcgtcga caacgtgcgc atggcggtcg cgtacttcgt cgacgccggt tacctcgaga
     1981 cgctcaagat cccgctgctc gccgggcgca ccttcggggc gcaggacacc cacgagacgc
     2041 cgccgggcct gatcatcgat caggccttcg ccgagaagtt cttccccggc caggacccgg
     2101 tcgggcagca cgtgc
//
LOCUS       DASWNJ010000415         3264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_323035, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000415 DASWNJ010000000
VERSION     DASWNJ010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323035"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="VGB85_07130"
     CDS             complement(<1..1116)
                     /locus_tag="VGB85_07130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0133834.1"
                     /translation="MSKTSRRPVFPWIAILLAHYLATGCGGDTPATTDGPVVAAKAPD
                     KSGVITIPQDSPMLSQIRVAPVSEEELAVDEVVSPAKVEIDPSRLSQVLLPVPGRVVQ
                     VHVHMGDAVKAGDVLLTLDSPDADAAIADHKQAEADLSAANAAMTKAKSDRERVRGLF
                     EKDAVARKEVDNVEAAFAAARSEVVRVQAERQQAQRKLKLLGITGKSKSGSETIDVRA
                     SISGKVLELHVVSGEFHSDTSKPLMTIADLSTVVVSADVAENLIRFIDVNEPVQIDLV
                     AFPGEIFAATVVRIADTVDPQTRTIEVLAALPNPEGRFRPEMFGRVRHGEATTRTPAL
                     PQTAVIQRDGQDIVLVETGPGQYVIRAVTLGTRAGDKV"
     gene            complement(1113..2369)
                     /locus_tag="VGB85_07135"
     CDS             complement(1113..2369)
                     /locus_tag="VGB85_07135"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY0133835.1"
                     /translation="MASTPNPVAVPADVPSYDERNALSVEEAVQEALEHNLSLLAERY
                     KVPMAQADQITARLRLNPVISFGADHLDILGTGYNDINKAGPQEFFARTDMFFLGGRK
                     RLRRMKVADNAVTVAELELRNEVRRLILDVQLTCTSVQLAEAEAQLASENLELFRGLV
                     KLNETRVAGGDLAKVELTRAQVAEMQASFDLRRAIAHRKTAQNNLGLLLGRTSTGREI
                     QVLGDMREDKTPVVLNEIRSEALRMRPDFRALRAEQVRSSYDIRRQMAEGKIDASVGV
                     EVRRQQGLAGTGNSMGVFVAVPIPMFNRNQGGVERARQENLAVQARIKAAEQGIRVEL
                     ENAFIAYQTARDLLDSFETGLLQKAQQVLGTATYAYRRGDASLIELLDAQRAFNETRR
                     SYNEARAAYANALYTIDAVTARRVPS"
     gene            2788..>3264
                     /locus_tag="VGB85_07140"
     CDS             2788..>3264
                     /locus_tag="VGB85_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133836.1"
                     /translation="MLDEDDTTTTSGGTGILSTPLGTDTTPTPTTTDAVTDGMESTSG
                     SPTTTGAGGTTFDPNDCGEAQIQIPIVTPNVMLVLDKSGSMVSMPSGYWDHDNDDPND
                     DGVKDSDPNMMEAATPRITRWQSLHEVVEFIVGAFDNSMNLGMVLFPSTTAKADYSA"
BASE COUNT          497 a         1120 c         1115 g          532 t
ORIGIN      
        1 gaccttgtcg ccggccctcg tgcccagcgt caccgcccgg atgacgtact ggcccggccc
       61 tgtctccacc agcacaatgt cctggccgtc gcgctggatc accgcggtct gcgggagcgc
      121 cggggtccgg gtcgtcgcct caccgtggcg gacccggccg aacatctcgg ggcggaagcg
      181 gccctcgggg ttcgggagcg ccgcgaggac ctcgatcgtg cgagtctgcg gatcgacggt
      241 gtcggcgatg cggaccaccg tcgccgcgaa gatctcgccc gggaacgcga cgaggtcgat
      301 ctgcacgggc tcgttgacgt cgatgaagcg gatcaggttc tccgcgacgt ccgccgagac
      361 gacgacggtg ctcaggtccg cgatcgtcat cagcggcttc gaggtgtccg agtgaaactc
      421 gccggacacg acgtgcagct ccaacacctt gccggagatc gaggcgcgca cgtcgatcgt
      481 ctcgctgccg gacttgctct tcccggtgat gcccagcagc ttgagcttgc gctgggcctg
      541 ctggcgttcg gcctggacgc ggacgacctc ggagcgggcg gccgcgaacg cggcctcgac
      601 gttgtcgacc tccttgcggg cgaccgcgtc cttctcgaac aggccgcgga cgcgctcacg
      661 atccgacttg gccttggtca tcgccgcgtt cgcggcactg aggtcggcct cggcctgttt
      721 gtggtcggcg atcgccgcgt ccgcgtccgg gctgtcgagc gtgagcagga cgtcgccggc
      781 cttgacggcg tcacccatgt gcacgtggac ctgcacgacc cgccccggga ccggcagcag
      841 cacctgcgag aggcggctcg gatcgatctc gaccttggct ggcgagacga cctcatcgac
      901 cgcgagctcc tcctcgctga ccggggcgac gcggatctgg gacagcatcg gcgagtcctg
      961 cgggatcgtg atgaccccgg acttgtcggg cgccttggcg gccactaccg gtccgtcggt
     1021 cgtggccgga gtgtcaccgc cacagcccgt cgccaggtaa tgagcgagaa ggatggcgat
     1081 ccacgggaac acgggccggc gcgatgtctt gctcatgatg ggactctccg cgcggtcacg
     1141 gcgtcgatgg tgtagagcgc gttcgcatag gccgcgcgcg cctcgttgta gctgcgtcgc
     1201 gtctcgttga aggcccgctg cgcgtcgagc agctcgatca ggctggcatc accgcggcgg
     1261 tacgcgtagg tcgcggtgcc gagcacctgc tgggcctttt gcaggagccc ggtctcgaag
     1321 ctgtcgagca ggtcgcgggc ggtctgatag gcgatgaacg cgttctcgag ctcgacgcgg
     1381 atgccctgct cggcagcctt gatccgggcc tgcaccgcga ggttctcctg gcgcgcccgc
     1441 tcgacgccgc cctggttgcg gttgaacatc gggatcggaa cggcgacaaa gacacccatc
     1501 gagttgcccg taccggcgag gccctgctgg cggcgaacct ccacgccgac gctggcgtcg
     1561 atcttgccct cggccatctg gcggcggatg tcgtagctcg agcggacctg ctcggcccgc
     1621 agtgcgcgga agtccgggcg catgcgcagc gcctcgctgc ggatctcgtt gagcacgacc
     1681 ggcgtcttgt cctcgcgcat gtctccgagc acctggatct cgcggccggt cgaggtccgc
     1741 ccgagcagca gcccgaggtt gttctgggcg gtcttgcggt gcgcgatcgc ccgccgcaga
     1801 tcgaagctcg cctgcatctc ggcgacctga gcccgcgtca gctcgacctt cgccagatcg
     1861 ccgccagcga cccgcgtctc gttgagcttg accagccccc ggaacagctc gaggttctcg
     1921 ctcgccagct gggcctccgc ctcggccagt tgcacggacg tgcacgtgag ctggacgtcg
     1981 aggatgagcc ggcgaacctc gttgcgcagc tcgagctccg cgacggtgac ggcgttgtcc
     2041 gcgaccttca tgcgacgcag gcgcttgcga ccgccgagga agaacatgtc ggtccgcgca
     2101 aaaaactcct gcgggcccgc cttgttgatg tcgttgtagc cggtgccgag gatgtccagg
     2161 tggtcggcgc cgaagctgat caccggattg aggcgcagac gcgccgtgat ctggtcggcc
     2221 tgcgccatcg gcaccttgta acgctcggcg agcagcgaca ggttgtgctc gagggcctcc
     2281 tggaccgcct cctcgacgga cagcgcgttg cgctcgtcgt aggagggcac gtcggccggg
     2341 acggccacgg ggttcggagt gctcgccacg accgggggcg tggagacacc cggccccgtg
     2401 ggcggcggag cggccagggc aggtcgagcc agcgccaagc ccgcgacccc tgccgcgcag
     2461 cacgcgcgca tggcgtgccg gcggggtaag gagggctgca agggtctcgg cacgcggccc
     2521 ctgtgtatgc caccccgcgc gccgtgccaa gaggcagggc cgcgcggttt tgtccggcgc
     2581 ctgggccttt tggggagcgc gagcttgctg gttggccgtt caactccgtc gtggcgacac
     2641 cgttctgcgg aacggaaaat ttcgcctcca cgaggtctaa gcaccccagc caggaacgct
     2701 cgctacgcga gccgaaaccc catgaaaccc accatccttg caaatctatt cttggtctct
     2761 gtcggtctgg ccgcttgcgg tgacaagatg ctcgacgaag acgacaccac gaccacgagc
     2821 ggtggcaccg gcatcctgtc cactccgctg ggaacggaca ccaccccgac cccgaccacc
     2881 acggatgcag tgacggatgg gatggagtcg acgagcggca gccccacgac caccggcgcc
     2941 ggcggcacca ccttcgatcc gaacgactgt ggtgaggcgc agatccaaat tccgatcgtc
     3001 acgccgaacg tcatgctcgt gctcgacaag tcgggctcga tggtctccat gccgagcggc
     3061 tactgggacc acgacaacga cgaccccaac gacgacggcg tcaaagacag cgaccccaac
     3121 atgatggagg ccgcgacgcc gaggatcacc cgctggcagt cgctgcatga ggtcgtcgag
     3181 ttcatcgtcg gtgccttcga caactcgatg aacctcggca tggtgctgtt cccctcgacg
     3241 accgccaagg ccgactacag cgcc
//
LOCUS       DASWNJ010000416         1817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_323087, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000416 DASWNJ010000000
VERSION     DASWNJ010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323087"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            267..1322
                     /locus_tag="VGB85_07145"
     CDS             267..1322
                     /locus_tag="VGB85_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133837.1"
                     /translation="MQLAPRGSPLREILLLGTCLALALPAPSVLASPASLPDWGAAAK
                     PAPKPEPAPAPAPAPAPVEATKPEPAAPAPAAAADDDIILPDPAPTPGPRRARRAGKP
                     GKPKPRANNKAAKTSLERAASERTPIQEHARSLETAGKLDEAVQSLLVGAQAYHDPVL
                     YLIAAEGTLKIAQSRGRAGVADDERAVEYVRTAQGLLQAADPAAPRVDPEEHATLHAW
                     GDDLVKQSERHRARMGVRRNGHGQLIAGGLLATAGLAGLGVMSGGLYLNNLSKRELAR
                     GEGRPEEELAPLRDQQKQGETMIAAGAVLGAVGLALGIALVSLGARDLKAARTETLQA
                     RVRVAPTFGGLVVVGRF"
     gene            1356..>1817
                     /locus_tag="VGB85_07150"
     CDS             1356..>1817
                     /locus_tag="VGB85_07150"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HEY0133838.1"
                     /translation="MRNRLDPIRVLTGVTLATVLNLSLGACSPVVLQDDTDTDGASSG
                     SGGSGGMSSGDTEGADGGGEGTVPEACVDLQLRVLGILETNCVQCHGAGSAGQGGIGY
                     MLDLNELIAQNKVVPGDAEGSRIYARMTALEAPMPPPTETQRPNATDTEIVG"
BASE COUNT          275 a          732 c          583 g          227 t
ORIGIN      
        1 gacgtcgcac agcagggcgc gaccgcctgt cacgagcgac cgacggcgcc ctgatcgccc
       61 ttggttgcac ataccaggaa agtcaaaaaa acctcacgcg ctcgtgatcg atgttcgtgc
      121 tccggttact tggggacatg aaacccggcg cgcgtgatcc ccctcgcgcg acgggccatc
      181 acccaccacc gtgatcccgc gggcgtcgcc aggcatcgat ctcacgatcg gcgccagctc
      241 ccgggttcgc gtcgagagga accgccatgc agctcgcacc acggggctcc cccttgcggg
      301 agatcctgct ccttgggaca tgtctcgcgc tggccctgcc tgcgccctcc gtgctcgcct
      361 cgccggcctc gttgcccgac tggggcgccg ccgccaagcc ggctccgaag cctgaacctg
      421 cgcccgcacc cgcacccgcg cccgcacccg tcgaggcgac aaagcccgag ccggccgcac
      481 ctgcgcccgc cgcagcggcc gacgacgaca tcatcctccc cgatccggcc ccgacccctg
      541 gcccacgccg ggcccgtcgc gccggcaagc ccggcaagcc caagccgcgc gccaacaaca
      601 aggccgcgaa gacctcgctc gagcgcgccg cctccgagcg cacgccgatc caggagcacg
      661 cgcgcagcct cgagaccgcc ggcaagctcg acgaggccgt gcagagcctg ctcgtcggag
      721 cgcaggccta ccacgatccg gtcctttatc tgatcgccgc cgagggcacc ctcaagatcg
      781 cccagagccg cgggcgagcc ggcgtcgccg acgacgagcg ggccgtcgag tacgtacgca
      841 ccgcccaggg cctgctgcag gccgccgacc ccgcggcccc gcgcgtcgac cccgaggagc
      901 acgccaccct gcacgcctgg ggcgacgacc tcgtcaagca gtccgagcgt caccgggctc
      961 gcatgggcgt gcgtcgcaac ggccacggcc agctgatcgc tggcggcctg ctggcgaccg
     1021 ccggcctcgc cggcctcggc gtcatgtccg gcggcctcta cctcaacaac ctctccaagc
     1081 gcgagctcgc caggggcgag ggccgccccg aggaggagct cgccccgctg cgtgaccagc
     1141 aaaaacaggg cgagaccatg atcgccgccg gcgccgtgct cggggccgtc ggcctcgccc
     1201 tcggcatcgc cctcgtctcc ctcggcgccc gcgacctcaa ggccgcgcgc accgaaacct
     1261 tgcaggcgcg cgtgcgcgtc gccccgacct tcggcggcct cgtcgtcgtc ggtcgcttct
     1321 gaccctccct ctcgttgaaa tctccaggag ttcccatgcg caaccgtctc gatcccatcc
     1381 gtgtcctcac cggcgtcacg ctggcaaccg tgctcaacct gtctctcggg gcatgttctc
     1441 ccgtcgtcct ccaggatgac accgacaccg acggcgcgag ctccggctcc gggggcagcg
     1501 gtggcatgtc cagcggggac accgagggcg ccgacggcgg gggcgagggc accgtgccgg
     1561 aggcctgcgt cgacctgcag ctgcgcgtgc tcggcatcct cgagaccaac tgcgtgcagt
     1621 gtcacggggc cggcagcgcg gggcagggcg gcatcggcta catgctcgac ctcaacgagc
     1681 tgatcgccca gaacaaggtc gtcccgggcg acgccgaggg ctcgcggatc tacgcgcgca
     1741 tgacggcgct ggaggccccg atgccgccgc cgaccgagac gcagcgcccg aacgccaccg
     1801 acaccgagat cgtcggc
//
LOCUS       DASWNJ010000417         3709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_290563, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000417 DASWNJ010000000
VERSION     DASWNJ010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290563"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..776)
                     /gene="cas2"
                     /locus_tag="VGB85_07155"
     CDS             complement(12..776)
                     /gene="cas2"
                     /locus_tag="VGB85_07155"
                     /inference="COORDINATES: protein motif:HMM:NF021356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEY0133839.1"
                     /translation="MRFHYIVSYDIADPTRLRRVHKAVRDFGDGVQLSVFACQITEHD
                     RAVLESKLLDITNQREDQVIFIKLGLVAEAQDAPPRCHSQAREVSIMPPKPPRPTTQE
                     IHRDEELVSCDLLRNLGRLLLDTLDALLLGGPQLDVRALHTEVAHILSDSDGHLRRLN
                     DARINRRGRAPTRTADAEPVPESARPFARQGSYLGSYASLQALAADALALGALVHPEL
                     ARLDLTGVGLDLHLHGIVWTIMADGSLHAFRPPPEE"
     gene            complement(782..1240)
                     /gene="cas1"
                     /locus_tag="VGB85_07160"
     CDS             complement(782..1240)
                     /gene="cas1"
                     /locus_tag="VGB85_07160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HEY0133840.1"
                     /translation="MGRSRRPPRDRVNALLSFGYSLLVRDATAAAARVGLDPMLGFLH
                     TIVPGRPALALDLMEPFRAAWVDTAVLRLIAIRGIDREDFVFTSAGVSLTDRGRNALI
                     GAYERRAEELTTHPRFGYRMAYRRLLELEARVLGKWLVGEVEHYTPIWTR"
     gene            complement(1270..2781)
                     /locus_tag="VGB85_07165"
                     /pseudo
     CDS             complement(1270..2781)
                     /locus_tag="VGB85_07165"
                     /inference="COORDINATES: protein motif:HMM:NF014040.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
     gene            complement(2799..>3709)
                     /locus_tag="VGB85_07170"
     CDS             complement(2799..>3709)
                     /locus_tag="VGB85_07170"
                     /inference="COORDINATES: protein motif:HMM:NF037736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEY0133841.1"
                     /translation="DAYETAIVRAQKYAKTLERDREFGVVGSRVGGSIGKGTAIKPVS
                     DVDLYLYLDEEAWQSTRGEALRPSTIIGRLHARVAQRLHFEIDAGHATIRRQGHSVGI
                     QFRKTGSVDIDVVPALVQGGDIREARIPRRGTDAFVATSVERQLELLGELDTRAKYLR
                     RGIRLLKLWKRDASVNLHSYAVEVIAMFVVTQGCKRTALGVFQAALEFMGTTNMRTPV
                     FIERYYDYRPHRRRVCTILDPAMPDNDLGAHLGSGAGDRLGAQARRSLRRLEKAARLA
                     EKGRLQGAADEVSEAFGEPGLITWHE"
BASE COUNT          555 a         1215 c         1383 g          556 t
ORIGIN      
        1 cgccagtcat tttactcctc ggggggcggt cgaaatgcgt ggagactccc gtcggccatg
       61 atggtccaca cgataccatg cagatggagg tccaggccga cgccggtgag atcgaggcga
      121 gcgagttcgg gatggacgag cgcgccgagg gcgagggcgt cggcggcgag ggcctgaagg
      181 gaggcatagg agccgagata gctcccctga cgggcgaacg gccgggcgga ctcgggcacc
      241 ggctccgcgt cggcggtgcg cgtgggggcg cggccgcggc ggttgatgcg ggcgtcgttg
      301 agccgccgga ggtggccgtc ggagtcggag aggatatggg cgacctccgt gtgcagcgcg
      361 cggacgtcga gctgcggacc gccgagcagc aaggcatcga gggtatcgag gaggaggcgg
      421 cccaggttgc gcaggagatc gcagctgacc agttcctcat cgcggtggat ctcctgggtg
      481 gtcggtcggg ggggcttggg tggcatgatg gaaacctctc gagcttgtga atgacagcgc
      541 ggcggggcgt cctgcgcctc cgcgaccagc cccagcttga tgaagatcac ctgatcttcg
      601 cgctggttgg tgatgtccag cagcttggat tcgaggaccg cgcggtcatg ctcggtgatc
      661 tggcaggcga acacgctgag ctggacgccg tcgccgaagt ctcgcacggc cttgtggacg
      721 cggcgcaggc gcgtcggatc ggcgatgtcg tagctgacga tatagtgaaa gcgcatggcg
      781 gtcaccgggt ccagatcggg gtgtagtgct cgacctcgcc gaccaaccat ttgccgagga
      841 cccgggcctc cagctcgagc aggcgacgat aggccatccg gtagccgaag cgcgggtggg
      901 tggtgagttc ctcggcgcgg cgctcgtagg cgccgatcag ggcgttgcga ccgcggtcgg
      961 tcaggctgac cccggcgctg gtgaacacga agtcctcgcg gtcgatgccg cggatggcga
     1021 tcaggcgcag gaccgcggtg tcgacccagg cggcccggaa gggctccatc aggtcgagcg
     1081 cgagcgcggg ccggcccggg acgattgtgt gaaggaagcc gagcatcggg tcgaggccga
     1141 cgcgagcggc cgccgcggtc gcgtcgcgca cgagcagcga gtagccgaag ctgagcagcg
     1201 cgttgacgcg gtcacgcggg ggccggcggc tgcgacccac gaagtcccgc tgccaggcgg
     1261 gggcaagatg gcagggagcg cggtgaagta gtacgcggcc gcctccccct cgcagccacg
     1321 cagcgcgtcg aggtcgccgg cctggctagc gcgccgcagg gcctgttgca ttgcgcgtag
     1381 cgcctgcacg cagcgcaccc gcggatccgc cgaggtgccg gcgagcccgg gcgaggtcgc
     1441 cgcggcctcg gtgagattct ccgtcagatc gagcgacggg tcttcgtcgg cgccgggcgc
     1501 cgtgagggcc ggcccatcgc tctcggagcg accccagtcg gggaggtcgc ccggctcctc
     1561 gcacagcgcc tgctcgacgc cccggccgta tcgccgcagg acggtccgct gattgcgaac
     1621 cttggcgagg accagggcgc gcgcggcctc gagggtgcgg gcggggtcgt ccgcggcgcg
     1681 aaactgggcc cggcgcaggt ggatgttctg cgacagcatc ggcgcggaca ggccgagcag
     1741 gcggcccgcg ctggtgtggt gagagaccgg gacgccctgc gtcaggagca actgcatgca
     1801 ttgctcggtg acctggacag gtccgtagat cgccacatac gagacgtctt tgagcaggac
     1861 gcgctcgggc tccgcgtcgt gcgggcaaat gacgagggac tcgccatcct ttcgcacgag
     1921 ggtgccgggg cgggtcacgt ggacgacggc gcggtcgtcc cggcccggga ggatgcgccg
     1981 gatctggcgc agctcgccgt cggccgcctc ggcctgggcc ttgagcgcgt gatgctcgac
     2041 aggcaggcag acgccgtgca gcgagcagcc gacgcacttg gggctcgact ccagcggctc
     2101 gggggcgacc gtcaggtcgc gtacgcggcg ggcctccgcg accacggccc gcaggaaccg
     2161 cgcgagctgg tcgctccagg cgatgatcgt gtgcacgccg ttgccgcggt aaaatagccg
     2221 gccctcgctg cacggtagac cggcgtcgcg cagggcgccc atgtatagac cgagctgggc
     2281 gacgtccggc ggccaggcgc ggtgcgggag atcgaagtct tgccagcggt gggtgtcggt
     2341 cgcgggggcc gagccgcgct tggcctcgac ggcgacgacc tcacctccga gctcgaccat
     2401 gtcgaggcgc ccatgaacgc cccaggccgg agcgctccac tccgtgctgc gagggatcag
     2461 ggcgtcccag gggagcgcga cgctggccgg ctgcgcggcg ctctccgcgt cggtaccaag
     2521 cttgcggcgg accttgccgc gccgcgcagc ccgctcgtgc tggccgctcc cctcgacggt
     2581 ctcggcggac gcgacaaaga tgccctggat gtgctcgatg tgaaacagcc gcgggcaata
     2641 gacgagctca ttgcacatgc ggatcgggct cgtatcttca gattcggtga tcatggtctg
     2701 atcacacagt cgcgcgccgg cgcgattcgg tccgccacgg ccggcgtatg ctctcgcaca
     2761 gatcaagggc gccgcggcca tgaatagagc gcggtcccct actcatgcca tgtgatgagc
     2821 cccggctcgc cgaacgcctc cgacacctcg tcggcggcgc cctgcaacct ccccttctcg
     2881 gccaggcgcg cggccttttc gagcctccgg agcgagcgac gagcctgcgc tcccagccgg
     2941 tcgccggccc cggagcccag gtgagcgcct aagtcgttgt ccggcatcgc cgggtcgagg
     3001 atggtacaga cgcgccgcct gtggggccgg taatcatagt accgctcgat gaagacaggc
     3061 gtgcgcatgt tggtcgtgcc catgaactcg agggccgcct ggaacacccc gagggccgtg
     3121 cgcttgcagc cctgcgtcac cacgaacatg gcgatcacct cgaccgcgta ggagtggaga
     3181 ttgacgctgg cgtctcgttt ccagagcttc agcagccgga tcccgcgtcg caggtacttg
     3241 gcccgggtgt cgagctcgcc gagcagctcg agctggcgct ccaccgaggt cgccacgaag
     3301 gcgtcggtgc cgcgccgcgg gatccgggcc tcccggatgt caccgccctg gaccagcgcg
     3361 gggaccacgt cgatgtccac ggagccagtc ttgcggaact gaatgccgac cgagtgcccc
     3421 tgcctgcgga tcgtggcgtg gccagcgtcg atctcgaagt gcagccgttg cgcgacccgg
     3481 gcgtgcaggc ggccgatgat cgtgctcggt cgcagcgcct cgccccgggt cgactgccac
     3541 gcctcctcat ccaggtagag atacaggtcg acgtcgctca ccggtttgat ggcagtacct
     3601 ttgccgatcg atccgccgac ccgggaccca acgacgccga actctcgatc tcgctcgagg
     3661 gtcttggcgt acttttgggc gcgcacgatc gccgtctcat aggcgtcat
//
LOCUS       DASWNJ010000418         2355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_501903, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000418 DASWNJ010000000
VERSION     DASWNJ010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501903"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VGB85_07175"
     CDS             complement(<1..320)
                     /locus_tag="VGB85_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133842.1"
                     /translation="MRLRPATIEKLARMICGEGSEYRDFPYRSGAGLTGFFTGLDFDY
                     IHSGSRLPWTVSTLQSINSTSPDTNTGVSNEMTFIICELLNPTNFEQSGNYPSALKRV
                     NLLL"
     gene            complement(547..1176)
                     /locus_tag="VGB85_07180"
     CDS             complement(547..1176)
                     /locus_tag="VGB85_07180"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0133843.1"
                     /translation="MKLYFHPLSGNSRRVLLVAAHLDVPLERIVVDLPRGEQRDAEHL
                     ARNPNGRIPVLDDDGFMLWESRAIMLYLAERTPGQTLLPADPRGRADVTRWLFWCAGH
                     MAPVNTIFVLENFVKPRTGRTTDPAELTRGEALMAQTAPILDAHLADRTWVTQDRLTL
                     ADLSLASSFALAAPARIPIAPYQNIQAWLARVQQLDAWQRTAPPPPNRD"
     gene            1282..>2355
                     /locus_tag="VGB85_07185"
     CDS             1282..>2355
                     /locus_tag="VGB85_07185"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0133844.1"
                     /translation="MVCATSVCALASPPGAPLDRAGKSGYQGDWALASSPMIRLYITP
                     DDQQPYLLSFERAHINVGSAEANDVRLQAPGISSRHFRLRSVGSEVVLEDGGSRNGTY
                     VNGVKVTGSQVLSPADVIRIVGFRIKILQPEAGVAATPAAPAAKPVAAAPVPRPAAPA
                     AVPRPAAPAAAPAAVPSPPARVEPVPAAVPVAAPVAAVVAAPAIDPAQEIDPPPGFDA
                     ARVAAIVGPRARAFSERADPSNLLRGEELQDAAIWVSCGKRVTPAAGVKERMLVARSQ
                     QVGQRHGRRRLGIAAAFGLALVGGGWTASSVLAGGPDAHAAGFSRAVVKQFLAAHDAQ
                     LRRSCEDSSRQLAEAALAEAKGSP"
BASE COUNT          351 a          703 c          910 g          391 t
ORIGIN      
        1 tgcagcaaaa gattcacgcg ctttagcgct gacggataat tgcccgattg ctcaaaattc
       61 gtcggattta ataattcaca tattataaat gtcatttcat tggatacgcc tgtgttcgtg
      121 tcaggagaag tgctgttgat cgattgaagc gtcgaaacag tccatggcaa ccttgagccg
      181 ctatggatgt agtcgaaatc aagccctgta aagaatcctg tcaaaccggc tcctgagcga
      241 tagggaaaat cacgatattc cgatccctca ccgcaaatca tgcgggcgag tttctcgatg
      301 gtagcgggtc gcaatcgcat ggctcatgct cgcacaccag gcgttggttg tcaccggtaa
      361 taccaagccg cgcgggtgcg cgtgccgcga tgggaatcgg ctgagcatcg agcgaagttg
      421 agtggttcgg atcgtgctgc tcgctgctct tcgagcgtcg tgaccccaac ctggccggag
      481 cgcagcccgt gcacggacga ctgcgcgctg cgctgtcgtc gactcgtttt tcgcgtcgag
      541 cgggtctcag tcgcggttcg ggggcggcgg tgcggtgcgc tgccaggcgt cgagctgctg
      601 gacgcgggcg agccaggcct ggatgttttg ataaggtgcg atcgggatgc gggcgggggc
      661 ggcgagggcg aaggagctgg cgagggacag gtcggcgagg gtcaggcggt cctgggtgac
      721 ccaggtgcgg tcggcgaggt gggcgtcgag gatcggggcg gtctgggcca tcagcgcctc
      781 gccgcgcgtg agctcggccg ggtcggtggt gcggccggtt cgcggcttca cgaaattctc
      841 gaggacgaag atcgtgttga ccggcgccat gtgaccggcg caccagaaca gccagcgggt
      901 gacgtcggcg cggccgcgtg ggtcggcggg gaggagggtc tgcccggggg tcctctccgc
      961 gaggtagagc atgatggcgc gcgactccca gagcataaag ccgtcgtcgt cgaggaccgg
     1021 gatgcgaccg ttggggttgc gcgcgaggtg ctcggcgtcg cgttgctcgc cgcgcggcag
     1081 gtcgacgacg atgcgctcga gcggcacgtc gaggtgcgcg gcgacgagga gcacgcggcg
     1141 ggagtttccg gacaggggat ggaagtagag cttcatgcgc cgtgaagtac gagagggcag
     1201 tgacaggata ttgtcaggat tgttttcatc accctggcgc acccgtcgcg ggcgacagcg
     1261 agccggggta tctgcgagat cgtggtgtgt gcaacgagcg tgtgcgcgct ggcgagcccg
     1321 ccgggcgcgc cgcttgatag ggccgggaaa tcgggctacc agggtgactg ggccctcgcc
     1381 tcgtcgccga tgatccgcct gtacatcacc ccggacgatc agcagcccta cctcctgagc
     1441 ttcgagcggg cgcacatcaa cgtcgggagc gcggaggcca acgacgtgcg cctgcaggcg
     1501 ccggggatct cgagccggca ctttcgtctg cgcagcgtcg gctcggaggt cgtgctcgag
     1561 gacggcgggt cgcgcaacgg cacgtacgtc aacggcgtga aggtcaccgg atcgcaggtg
     1621 ctgtcgcccg cggatgtgat ccgcatcgtc ggctttcgca tcaagatctt gcagcccgag
     1681 gccggggtgg ccgcgacgcc ggctgcaccc gctgcgaagc cagtcgcggc ggcgcccgtg
     1741 ccgaggccgg ctgcgcctgc tgcggtgccg aggccggccg cgcctgctgc cgcgccggct
     1801 gcggtgccga gtccgcctgc gagggtcgaa ccggtgcccg cggcggtgcc ggtggcggct
     1861 ccggtcgctg ctgtggtggc cgcgcccgcg atcgatccgg cgcaggagat cgatccgccg
     1921 ccgggcttcg acgcggcgcg ggtggccgcg atcgtcgggc cgcgggcgcg ggcgttctcc
     1981 gagcgtgccg acccgagcaa tctcctgcgc ggcgaggagc tgcaggacgc ggcgatctgg
     2041 gtcagctgtg gcaagcgcgt gacgccggcg gcgggcgtga aggagcggat gctcgtcgcg
     2101 cgcagtcagc aggtcgggca acggcacggg cggcggcggc tcggcatcgc ggctgcgttc
     2161 gggctggcgc tcgtcggcgg cggctggacg gccagcagcg tgctcgccgg agggcccgac
     2221 gcgcacgcgg ccgggttcag tcgcgcggtc gtgaagcagt tcctcgcggc tcatgacgcg
     2281 cagctgcggc gctcgtgcga ggactcgtcg cgccagctcg cggaggcggc gctggcggag
     2341 gcgaagggct cgccg
//
LOCUS       DASWNJ010000419         6060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_394891, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000419 DASWNJ010000000
VERSION     DASWNJ010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394891"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VGB85_07190"
     CDS             complement(<1..583)
                     /locus_tag="VGB85_07190"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY0133845.1"
                     /translation="MAFEPTLRELSRSALTLVLAGGRGSRLGALTDRRAKPAVHFGGK
                     LRIIDFALSNALNSGFRQMAVLTQYKSHSLLRHLQRGWSFLRGEMGEFVDLLPAQQRI
                     NEENWYRGTADAVYQNLDIFRSVQPRHVIILAGDHVYKMDYGKMLLAHVERGADATVA
                     CIEVPRMEATAFGVMHIDEDDRITAFLEKPADPP"
     gene            complement(654..1649)
                     /gene="corA"
                     /locus_tag="VGB85_07195"
     CDS             complement(654..1649)
                     /gene="corA"
                     /locus_tag="VGB85_07195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEY0133846.1"
                     /translation="MFTLHVFHATRGHWIEPMTSGDCVQALVKLRADPEATIWLDLQE
                     PTEVESRVLSEVFHFHPLAIEDTLQDYGHPKIDSFDDHVFMIVHGIDFTRLNLEKSLD
                     VFTLELDIFLGARYIVTHHGDPAMRSVADLHKDVGEPGHRPWSSVRILHRLLDRLVDN
                     YLPVMDGIGAKLEILEDQIIHNPEPDLLEKVLEAKKSIQRIRRMTAHQRLILESLARG
                     HLELIPRESLAFFRDIYDHFVRVADLAEAYREGAQSAVEAYLSMSANKTNEIMKVLTQ
                     ISTIMLPLTFIAGIYGMNFDHMPELHWLYGYPFAVSLMVVTAVALLVWFRRRHWL"
     gene            1730..1954
                     /locus_tag="VGB85_07200"
     CDS             1730..1954
                     /locus_tag="VGB85_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133847.1"
                     /translation="MRPRLSVWASVWAMVSAVAACTTPGCTTTPRVDGPHRRARRSWR
                     HDLSYFARTATPLVFVRAPARGVPVNDIGA"
     gene            complement(1962..3134)
                     /locus_tag="VGB85_07205"
     CDS             complement(1962..3134)
                     /locus_tag="VGB85_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133848.1"
                     /translation="MPLELFAVTQPGLEDICAAEVAELGLGPGTTVPGGVTFTGEAVQ
                     LATANLRLSVATRVLVRVAEFRAPGFPELHARAGKLAWERFIPADAPPRLCFHVSAHK
                     SRLYHTGGIAERVAKGIGERLGSPPEMIAPGDDDAEAEDDTRAPTRIYVRLDHDRCTV
                     SVDSSGDPLHRRGYRLALARAPLRPTLAAAMLRACGWQPGAPLLDPMCGSGTIVLEAA
                     RRACGRPPGGDRQFAFLAWPGLPGAAALAQARGDLSPGTADAAPGLAATLAPIVGSDR
                     DPGAITAARANAERAGVADAVTLAVRPLSAIEPLPAPGFVVCNPPYGVRVGKRGPLRD
                     LYAQLGHVLRDRCPGARVALLCGDDLWHHCGLPLGITHHLKNGGLPVQLVQGRVPA"
     gene            complement(3168..3689)
                     /locus_tag="VGB85_07210"
     CDS             complement(3168..3689)
                     /locus_tag="VGB85_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY0133849.1"
                     /translation="MRRLLLILAPLALACGDSGGGDDDSGDSTGGATGSTPGTDPTTT
                     ATDDPDEAATQLCVDTINMYRATLGLAPLGRWKDAEDCSDAEAASDGKTGTPHGAFGM
                     CGEGAQNECPGWPGPPASMIPGCLELMWSEGPGEDFAAHGHYINMSSTSFTRVACGFA
                     EGDGGVWSVQNFQ"
     gene            complement(3726..4061)
                     /locus_tag="VGB85_07215"
     CDS             complement(3726..4061)
                     /locus_tag="VGB85_07215"
                     /inference="COORDINATES: protein motif:HMM:NF013089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEY0133850.1"
                     /translation="MSPATMAWVYLCTAGVLEIAWAVGMKLSEGFKKLEIAAPTVLAM
                     ILSFYFLSLALRTLPLGTAYAIWTGIGAIGTLAVSMIWFDEPFGWLRVMFATMAIAGL
                     AGLKVTSGH"
     gene            complement(4058..5020)
                     /locus_tag="VGB85_07220"
     CDS             complement(4058..5020)
                     /locus_tag="VGB85_07220"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0133851.1"
                     /translation="MQYTQLGRSGLVVSRIGLGCMSYGDPAWRPWVLDETAAMPFFRR
                     AIEAGINFFDTADMYSLGASEVITGKALREYGRRDELVIATKVLYPMGDGPNMRGLSR
                     KHIMQACEASLRRLGVEAIDLYQVHRFDPTVPLEETLAALDLLVTQGKVRYLGASSTA
                     AWRLAKALYLADARGLARFVSMQNHYNLLYREEEREMLPLCREEGLGVIPWSPLARGL
                     LAGASTTSRAGSDTITPQLYDDPADEAVIAANAAVARARGVSPAQTALAWLLHQPSVS
                     APIVGATKLAHLEAALAAVDLQLTPDERGALEAPYRPHAVRGHQ"
     gene            5133..>6060
                     /gene="pyk"
                     /locus_tag="VGB85_07225"
     CDS             5133..>6060
                     /gene="pyk"
                     /locus_tag="VGB85_07225"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY0133852.1"
                     /translation="MPQPQQRRAKIVCTLGPASSDPEMISALIGAGMNVARLNMSHGT
                     QEDHTRVFEAIRQSAASQGVAVAVLADLQGPKIRVGKIPGQGFDLKAGDMFVLSVDPN
                     AVIEPGRVTVDYPEMAREVKPDDKILMDDGNFELRVVAVEGAEIKTCVITGGRLTSRK
                     GVNLPGVQLSLPSLTNKDKDDLRFALELGVDTVALSFVRTAGDILEVQALMEQAGRVV
                     PIIAKIEKPEGVTNLKEILAVANGIMVARGDLGVEMGPEEVPIIQKQAIELANRAGKL
                     VITATQMLDSMIRNPRPTRAEASDVANAVLDGS"
BASE COUNT          894 a         1980 c         2303 g          883 t
ORIGIN      
        1 cgggcgggtc ggccggcttc tccaggaaag cggtgatccg gtcgtcttcg tcgatgtgca
       61 taacgccgaa ggcggtcgcc tccatgcgcg gcacctcgat gcaggcgacc gtcgcgtcgg
      121 ccccgcgctc gacgtgcgcg agcaacatct tgccgtagtc catcttgtag acgtggtcac
      181 ccgcgaggat gatgacgtgc ctgggctgga cgctgcggaa gatgtcgagg ttctggtaca
      241 cggcgtcggc ggtgccgcgg taccagttct cctcgttgat gcgctgctgc gcgggcagca
      301 ggtcgacgaa ctcgcccatc tcgccgcgca ggaagctcca gccgcgttgc aggtggcgca
      361 ggaggctgtg cgacttgtac tgcgtcagca cggccatctg gcgaaagccg gagttgaggg
      421 cgttcgacag ggcgaagtcg atgatgcgca gcttgccgcc gaagtggacg gccggcttgg
      481 cgcggcggtc ggtgagggcg ccgaggcgag agccgcggcc gccggccagc accagggtca
      541 acgccgatcg gctcagctcg cgcagggtcg gttcgaaagc catgtgtggg tcccgctcca
      601 ggctctatca cccccgtggc ccgcggatca acttctggcc ggaccggggt tgttcacagc
      661 cagtggcggc ggcggaacca caccagcagc gcgacggccg tcacgaccat gaggctgacc
      721 gcgaaggggt agccgtacag ccagtgcagc tcgggcatgt ggtcgaaatt catgccgtag
      781 atgccggcaa taaaggtcaa gggcagcatg atcgtcgaga tctgcgtcag gaccttcatg
      841 atctcgttgg tcttgttggc cgacatcgac aggtaggcct cgaccgccga ctgcgcgccc
      901 tcgcggtagg cctcggcgag gtcggcgacg cggacgaagt ggtcgtagat gtcgcggaag
      961 aacgccagac tctcgcgcgg gatcagctcc aggtgcccgc gcgcgagcga ctcgaggatc
     1021 aggcgctggt gggcggtcat gcggcggatc cgctggatcg acttcttggc ctcgagcacc
     1081 ttctccagca ggtcgggctc ggggttgtgg atgatctggt cctcgaggat ctcgagcttg
     1141 gcgccgatcc cgtccatcac cgggagatag ttgtcgacca gacggtcgag caggcggtgc
     1201 aggatccgga ccgagctcca gggcctgtgg ccgggctcgc cgacgtcctt gtggaggtcc
     1261 gccaccgagc gcatcgccgg gtccccgtgg tgcgtcacga tgtaacgggc gccgaggaag
     1321 atgtcgagct cgagcgtgaa cacgtcgagg ctcttttcga ggttgaggcg cgtgaagtcg
     1381 atgccgtgca cgatcatgaa cacgtgatcg tcgaagctgt cgatcttcgg gtggccgtag
     1441 tcctgaagcg tgtcctcgat cgccagcggg tggaagtgaa acacctccga cagcacgcgc
     1501 gactcgacct ccgtcggctc ctgcagatcg agccagatcg tcgcctcggg gtcggcgcgg
     1561 agcttgacca gcgcctgcac gcagtcgccc gaggtcatcg gctcgatcca gtggccacgt
     1621 gtcgcgtgga agacgtggag agtgaacacg gggcgcaggg taccacgcag ggctgcgcgc
     1681 gcccgcgggc tttttgccgc gcgcgtcggc gttgaacgcc tgcatgtata tgcggcctcg
     1741 actcagcgtg tgggcgagcg tgtgggcgat ggtgagcgcc gtcgctgcgt gcacgacccc
     1801 cgggtgtacg acgacgccgc gcgtcgacgg gccgcaccgg cgagcgcgcc ggtcgtggcg
     1861 tcacgacctg agctacttcg ccaggacggc gacgccgctg gtgttcgtgc gcgcgccggc
     1921 gcgtggggtc ccggtgaacg acatcggggc gtgagcccga gtcacgcggg tacgcggccc
     1981 tgcacgagct gcaccggcag gccgccgttc ttgaggtggt gcgtgatgcc cagcggcagg
     2041 ccgcagtggt gccagaggtc atcgccgcac agcagcgcga cgcgggcgcc ggggcacctg
     2101 tcccgcagga catgtccgag ctgggcgtag aggtcgcgca gcgggccgcg cttgccgacc
     2161 cgcacgccgt agggcgggtt gcagacgacg aagcccgggg ccggcagcgg ctcgatggcg
     2221 gacagcgggc ggaccgcgag cgtgacggcg tcggcgaccc ccgcccgctc ggcgttggcg
     2281 cgcgcggccg tgatcgcgcc ggggtcgcgg tcggagccga cgatcggtgc gagagtcgcc
     2341 gcgagacccg gtgcggcatc ggctgtcccc ggggacaggt cgccgcgcgc ctgggcgagc
     2401 gcggcggcgc cgggcaggcc gggccaggcg aggaacgcga attgtcggtc gccgccgggc
     2461 gggcgcccgc aggcccggcg cgccgcctcg agcacgatcg tgccgctgcc gcacatcggg
     2521 tcgagcagcg gggcgcccgg ctgccagccg cacgcgcgca gcatcgcggc cgcgagggtc
     2581 gggcgcagcg gggcgcgggc cagcgcgagg cgatagccgc ggcgatgcag cgggtcgccg
     2641 gagctgtcga cgctgacggt gcaacgatcg tgatcgaggc gcacgtagat gcgggtcggc
     2701 gcgcgcgtgt cgtcttcggc ctcggcgtcg tcgtcacccg gggcgatcat ttcgggtgga
     2761 gacccgaggc gctcgccgat gccctttgcg acgcgctcgg cgatgccgcc ggtgtggtag
     2821 agccgcgact tgtgggcgct gacgtggaag cacagccgcg gtggcgcgtc ggccgggatg
     2881 aagcgctccc acgccagctt gcccgcgcgt gcgtgcagct ccgggaagcc gggcgcgcgg
     2941 aactcggcga cccgcacgag cacgcgcgtg gcgaccgaca gccgcaggtt ggcggtcgcc
     3001 agctgcacgg cctcgcccgt gaacgtgacg ccgccgggca cggtcgtgcc gggcccgagg
     3061 ccgagctctg ccacttcggc ggcgcagatg tcctcgaggc caggttgagt gacggcgaac
     3121 agctcgaggg gcacgcgcgc gcaggatagc cgccggaggc ggtcgcgtca ctggaagttc
     3181 tgcaccgacc agaccccgcc gtcgccctcg gcgaagccgc aggcgacccg ggtgaagctg
     3241 gtgctgctca tgttgatgta gtgcccgtgg gcggcgaagt cctcgccggg accctcgctc
     3301 cacatcaatt cgaggcagcc ggggatcatg ctcgcgggcg gtccgggcca gccggggcac
     3361 tcgttctggg ccccctcgcc gcacatgccg aacgcgccgt gcggggtgcc cgtcttgccg
     3421 tcgctggcgg cctcggcgtc ggagcagtcc tcggcgtcct tccagcgccc cagcggcgcg
     3481 aggcccagcg tggcgcggta catgttgatc gtgtcgacac acagctgcgt cgcggcctcg
     3541 tccgggtcgt cggtggcggt ggtcgtcggg tccgtgccgg gggtgctgcc ggtggctccg
     3601 ccggtactgt cgccggagtc gtcgtcgccg ccgccggagt ctccgcaggc gagggcgagc
     3661 ggggcgagga tcaggagcag gcggcgcatg tcggcacggt accagagcac cgcgcgcgcg
     3721 tgcgctcagt ggcccgaggt gaccttgagg ccggcgagcc cggcgatcgc catcgtcgcg
     3781 aacatgacgc gtaaccagcc aaagggctcg tcgaaccaga tcatgctgac ggcgagcgtc
     3841 ccgatcgcgc cgatgccggt ccagatcgcg taggcggtgc cgagcggcag ggtgcgcagc
     3901 gcgagcgaga ggaagtaaaa gctcaggatc atggcgagca ccgtcggcgc cgcgatctcg
     3961 agctttttaa aaccctcgct gagcttcatg ccgacggccc acgcgatctc gaggacgccg
     4021 gcggtgcaca ggtagaccca ggccatcgtc gcggggctca ttgatgacct cgcacggcgt
     4081 ggggtcggta gggggcctcg agcgcgccgc gctcgtcggg cgtcagctgg aggtcgaccg
     4141 ccgcgagcgc ggcctcgagg tgagcgagct tcgtggcgcc gacgatcggg gcgctgacgg
     4201 acggctggtg gagcagccac gcgagggccg tctgcgccgg gctgacacca cgggcgcggg
     4261 cgacggccgc gttggcggcg atcacggcct cgtcggcggg atcatcgtag agctgcggcg
     4321 tgatcgtgtc gctgccggcc cgcgaggtcg tgctggcgcc cgcgagcagg ccgcgcgcga
     4381 gcggcgacca ggggatgacg ccgagcccct cctcgcggca cagcggcaac atctcgcgct
     4441 cctcctcgcg gtacagcagg ttgtagtggt tctgcatgct gacgaagcgc gcgaggcccc
     4501 gggcgtccgc gaggtacagc gccttcgcca gccgccaggc ggcggtcgag ctggccccga
     4561 ggtagcgcac cttgccctgc gtgaccagca ggtcgagcgc ggccagggtc tcttccagtg
     4621 ggacagtcgg gtcgaagcgg tgcacctgat acaggtcgat cgcctcgacg ccgaggcggc
     4681 gcaggctggc ctcgcaagcc tgcatgatgt gcttgcgcga gaggccgcgc atgttggggc
     4741 cgtcgcccat cggatagagc accttggtgg cgatgacgag ctcgtcgcgg cgtccgtact
     4801 cccgcagcgc cttgccggtg ataacctcgc tggcgccgag cgagtacatg tcggcggtgt
     4861 cgaagaagtt gatgcccgcc tcgatcgcgc ggcgaaaaaa cggcatcgcc gcggtctcgt
     4921 cgagcaccca cggccgccac gcggggtcgc cgtagctcat gcagccgagg ccgatccggg
     4981 agacgacgag gccgctgcgg ccgagctggg tgtactgcat ggcggccggg aaggtagcac
     5041 gtggcccggt cgtgtcaggg caggcgctct ggccgcccgg ggcccgtgcc ggggggtgcc
     5101 cgggcgagcg gtgttctggc atgatgcgcg gcatgcctca gcctcagcag cgtcgcgcca
     5161 agatcgtgtg cacccttggt cctgccagca gtgatccgga gatgatctcg gcgttgatcg
     5221 gcgccgggat gaacgtcgcg cgcctcaaca tgtcgcacgg cacgcaggag gaccacacgc
     5281 gggtcttcga ggcgattcgc cagagcgcgg cctcgcaggg ggtggcggtc gcggtgctcg
     5341 cggacctgca gggcccgaag atccgcgtcg gcaagatccc cgggcagggc ttcgacctca
     5401 aggccggcga catgttcgtg ctcagcgtcg accccaacgc ggtgatcgag cccgggcgcg
     5461 tgacggtcga ctatccggag atggcgcgcg aggtcaagcc ggacgacaag atcctgatgg
     5521 acgacggcaa cttcgagctg cgcgtcgtcg cggtcgaggg cgcggagatc aagacctgcg
     5581 tgatcacggg cgggcgcctg acctcgcgca agggcgtcaa cctgccgggc gtgcagctga
     5641 gcctgccgtc gctgacgaac aaggacaagg acgacctgcg cttcgcgctc gagctcggcg
     5701 tcgacacggt cgcgctgtcg ttcgtgcgca cggcgggcga catcctcgag gtccaggcgc
     5761 tgatggagca ggcggggcgg gtggtcccga tcatcgccaa gatcgagaag ccggagggcg
     5821 tcaccaacct caaggagatc ctcgcggtcg ccaacggcat catggtggcg cgcggcgacc
     5881 tcggggtcga gatgggcccc gaggaggtcc ccatcattca aaagcaggcg atcgagctcg
     5941 ccaaccgggc cggcaagctc gtgatcacgg cgacgcagat gctcgactcg atgatcagga
     6001 acccgcggcc gacccgcgcg gaggcctcgg acgtcgccaa cgcggtgctc gacggcagcg
//
LOCUS       DASWNJ010000420         3183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_219698, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000420 DASWNJ010000000
VERSION     DASWNJ010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219698"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..2332
                     /locus_tag="VGB85_07230"
     CDS             152..2332
                     /locus_tag="VGB85_07230"
                     /inference="COORDINATES: protein motif:HMM:NF017315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HEY0133853.1"
                     /translation="MPPSARITDMHTCPLPTHVGGPVNVGEPTVLIGNVPAARVSDSL
                     VCACGPDMISQGEATVLIGHMPAARLGDATGHGGVLAQGDPTVLIGSTAQAEALRTDK
                     PFCEECERARQAAEHRGWPPVLIDAHMHIQSGNCAPLPPVHALILNIRLGRGIIGWVG
                     RAFLGEFGDFAAEPTHVIADRLVSHNEGLRDLMQGEGAYFLGMSVVLTMDMDYCHVDG
                     YKGEHVYYEDEQGDRYYLKRHKRRGGAKHRDDPWPPPATRGDRSYVGRREDAPMDLEE
                     GEVPDPEDRLLEDYTRDLRAAMYETWRQQRRRTEMACVRHPLRLLPMYHYEPRRYISE
                     TPPSQPYEQLVEAGGLYVGFKMYTSLGYMPDETSEAGAKVQAITRDLFKQCAARDIPV
                     MTHCTPSGFYTHQRELYIELATPAKRDEYRGADGELADRWRLKYFQDHCVHPEAWRPV
                     LAQNPSLRLCLAHWASDSHVWLDEVAEFRKPPLTLAQLHAVDRQIDERRADAMVLGLP
                     VPARAEALLAMFREGDPYPGSARPCIDDNGSLYGKGWVRSIVELCGTYPNFFTDISFL
                     PLFKEIKRPGGEKFYQWEVVADILKEYPYMVDHIMFGTDWYMILNAKYAYREWFVKTK
                     ECLQHIQARLGGAWAEHDIFHQWAIVNPIRFYRLPKIAAKLKDNLASRIAAEPGVDHA
                     ALRRDLERRWEVLSAAVDEGRLAAMEAAIKAGPIGFSPPPGFLP"
     gene            2329..>3183
                     /locus_tag="VGB85_07235"
     CDS             2329..>3183
                     /locus_tag="VGB85_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133854.1"
                     /translation="MILQPGPGTTSALVSPGGRAEFPVDPGEVGQVLPLLSPAGVALL
                     SYDEVLLRVRVEARGATIVEQVRGYLPSIAGGELFALTPVAPSAACTYLQSRRLVRYD
                     FDAHDHRVDMVAHGEEMAVAAAWLSPREDAVVVQIEDTSRYDEGGLTLSRLVGFDLTG
                     ARARRIGAIQLPRLIARGRGWSAGGGLLGVIVEGELQVLDAGLQRRDDHPLAQAARAL
                     TAEAPGVLQELRIHPHAPLALLSVQERDLLGLRDHALWWVRWSGDTVERRLACRLRAI
                     GGLALGAFA"
BASE COUNT          457 a         1124 c         1159 g          443 t
ORIGIN      
        1 cggggtggtc atccagggcc agatggtccg catcaacagc ggcggcgcgg cgctggcggg
       61 cagcggctcc gagccgcagc tcccggacga cccgctgcac gcccagccgg tgacccccaa
      121 gggcgccgac cacgggctgg aggcctgacc gatgccgccc tcagcgcgca tcacggacat
      181 gcacacctgc ccgctgccga cccacgtcgg cgggccggtc aacgtcggcg agccgacggt
      241 gttgatcggg aacgtcccgg cggcgcgcgt cagcgactcg ctggtgtgcg cgtgcgggcc
      301 cgacatgatc agccagggcg aggcgaccgt cctgatcggc cacatgccgg cggcgcgcct
      361 cggcgacgcg accggacacg gcggtgtgct ggcgcagggc gacccgacgg tgctgatcgg
      421 ctcgacggcg caggcggagg cgctgcgcac cgacaagccg ttctgcgagg agtgcgagcg
      481 ggcccgccag gcggccgagc accgcggctg gccgccggtg ctgatcgacg cgcacatgca
      541 catccagtcg ggcaactgcg ccccgttgcc gccggtccac gcgctgatcc tcaacattcg
      601 cctcgggcgc gggatcatcg gctgggtcgg gcgcgcgttc ctcggggagt tcggcgactt
      661 cgcggccgag ccgacgcacg tgatcgccga tcgcctcgtc agccacaacg agggcctgcg
      721 cgacctgatg cagggcgagg gcgcgtactt cctcggcatg tcggtggtcc tgacgatgga
      781 catggattac tgccacgtcg acggctacaa gggcgagcac gtctattacg aggacgagca
      841 gggcgatcgc tactacctca agcgccacaa gcggcggggc ggcgcgaagc accgggacga
      901 cccgtggcca ccgccggcga cccgtggcga ccgcagctat gtcgggcgcc gcgaggacgc
      961 gcccatggat ctcgaggagg gcgaggtgcc cgatcccgag gaccggctgc tcgaggatta
     1021 cacgcgcgac ctgcgggcgg cgatgtacga gacctggcgg cagcagcggc gccgcaccga
     1081 aatggcgtgc gtgcgccacc cgctgcggct gctgccgatg tatcactacg agccgcgccg
     1141 ctacatcagc gagacgccgc cgtcgcagcc ctacgagcag ctggtcgagg cgggcgggct
     1201 ctacgtcggc ttcaagatgt acacctcgct cggctacatg ccggacgaga cgagcgaggc
     1261 cggcgcgaag gtccaggcga tcacgcgcga cctgttcaag caatgcgccg cgcgtgacat
     1321 cccggtgatg acacactgca ccccgagcgg cttttatact caccagcgcg agctctacat
     1381 cgagctggcg acgccggcga agcgcgacga gtaccgcggg gccgacggcg agctcgcgga
     1441 ccgctggcgg ctcaagtact tccaggacca ctgcgtgcac ccggaggcgt ggcggccggt
     1501 gctcgcgcag aacccgtcgc tgcggctgtg cctggcccac tgggcgtcgg actcgcacgt
     1561 gtggctcgac gaggtcgccg agtttcgcaa gccgccgctg acgcttgcgc agctgcacgc
     1621 ggtcgaccgc cagatcgacg agcgccgcgc ggacgcgatg gtcctcgggt tgccggtccc
     1681 ggcgcgcgcc gaggcgctgc tcgccatgtt tcgcgagggc gacccgtacc ccggcagcgc
     1741 tcgcccgtgc atcgacgaca acgggtcgct gtacggcaag ggctgggtgc ggtcgatcgt
     1801 cgagctgtgc ggcacgtacc ccaacttttt cacggacatc tcgttcctcc cgctgttcaa
     1861 ggagatcaag cggccgggcg gcgagaagtt ctaccagtgg gaggtcgtcg ccgacatcct
     1921 caaggagtac ccgtacatgg tcgaccacat catgttcggc accgactggt acatgatcct
     1981 caacgccaag tacgcgtacc gcgagtggtt cgtgaagacg aaggagtgcc tgcagcacat
     2041 ccaggcccgc ctcggcggcg cgtgggccga gcacgacatc ttccaccagt gggcgatcgt
     2101 caacccgata cgcttctatc gcctgccgaa gatcgccgcg aagctcaagg acaacctcgc
     2161 cagccgcatc gccgccgagc cgggggtcga ccacgccgcg cttcgccgcg acctcgagcg
     2221 ccgctgggag gtgctgtccg ccgcggtcga cgagggccgc ctcgcggcga tggaggcggc
     2281 gatcaaggcc ggaccgatcg gctttagccc gcccccgggg ttcctgccgt gatccttcaa
     2341 cccggtccag gcacgacgtc ggcgctggtg agcccgggcg gccgcgccga gtttccggtc
     2401 gacccggggg aggtcgggca ggtcctgccg ctgctctcgc cggccggcgt cgcgctgctg
     2461 tcgtacgacg aggtgctgct gcgcgtgcgt gtcgaggctc gcggcgcgac gatcgtcgag
     2521 caggtccgcg gctatctgcc gtcgatcgcc ggcggcgagc tgttcgcgct gaccccggtc
     2581 gcgccctcgg ccgcctgcac ctacctgcag agccgccgtc tggtccgcta cgacttcgac
     2641 gcgcacgacc accgcgtcga catggtcgcg cacggcgagg agatggcggt cgcggccgcg
     2701 tggctgagcc cgcgcgagga cgcggtggtc gtgcagatcg aggacacgag tcgctacgac
     2761 gagggcgggc tgacgctgtc acgtttggtc ggcttcgacc tgaccggtgc gcgcgcgcgc
     2821 cggatcgggg cgatccagct tccgcggctg atcgcccgcg ggcgcgggtg gtcggccggg
     2881 ggcgggctgc tcggcgtgat cgtcgagggc gagctgcagg tcctcgacgc cggcctgcag
     2941 cgccgcgacg accacccgct cgcgcaggcc gcccgcgcgc tgacggccga ggcgcccggc
     3001 gtgttgcagg agctgcggat ccacccccac gccccgctcg cgctgctatc ggtgcaggag
     3061 cgcgacctgc tcggcctgcg cgaccacgcg ctgtggtggg tccgctggtc gggggacacg
     3121 gtcgagcgcc ggctcgcgtg ccggctgcga gcgatcggcg ggctcgcgct cggggccttc
     3181 gcg
//
LOCUS       DASWNJ010000421         2693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_394921, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000421 DASWNJ010000000
VERSION     DASWNJ010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394921"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1340
                     /locus_tag="VGB85_07240"
     CDS             <1..1340
                     /locus_tag="VGB85_07240"
                     /inference="COORDINATES: protein motif:HMM:NF028473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133855.1"
                     /translation="GGSELTPAGMFTVPPTAGTWTVRATLTADPQIIDDATVVADFRG
                     GRLVDGWGPEGEGLWAHEVVETSQAADLVADAEHIYALIRHTAPPSATLVSLVEATGL
                     GKPGFGSGGVRSYTAPGGVKIDPRQLGLAADRLYIQGQVVLDTVVSAATLDSGQPIND
                     FGTHKSQYSMRGATPDPSSGTVYVGIDDFGVVGAERLFPNGDVDMTWGDAGKRLLAAP
                     SAWKSTWDPGKELLVLRELEVDSSGRLYALLTSFDEANTGEVALVMRLDTSGALDPSF
                     GLGGAALWAIDDQPGTDPKRMQITADGGIVVVGADYGQWFATRLTADGDLDLGWGDGG
                     HVHHAYMRDIGSPASTAGVDVAVMPDGHVVFLINIEPASELTALVRYTQDGQVDLDFG
                     DPEADGDGWSLIDTAPVRLAPGVDGRVFVLGQFTYSDSDVTISDAWINAVHTY"
     gene            1663..>2693
                     /locus_tag="VGB85_07245"
     CDS             1663..>2693
                     /locus_tag="VGB85_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133856.1"
                     /translation="MTKTSSLFVLGLGASLWGCNGGGSAGTETDGSTGVTSIVPTNPT
                     TTGTTGSTATTSGPTEPTGPSEPTAGTNDTTAAATEATGDTGTTEPAADTTGDTGTTG
                     PAGDTTGDTTGAEEVCAAPTTYITCDAALDPADPLAPFQAMGLGCPGDASDSITISEP
                     SFNSQNPNAWRIATGFGSYHEDNDPDKPLYFSPREGGSFLIISTGVVAQADPAGVVVE
                     AAKSQDDNGNNGNDDADELPPPFNVVPGSNGGAGGTPFLDCDGVGDCSDTLQTQWNLG
                     EANPNDKLWFSFKATVPMGTHGYVFNFAYCSAEYPMYIDYKYNDMVVAWQISEAYTGN
                     VTFVKYMDE"
BASE COUNT          448 a          951 c          944 g          350 t
ORIGIN      
        1 cgggcgggtc ggagctgacg ccggccggta tgttcacggt gcccccgacg gccgggacgt
       61 ggacggtgcg cgcgacgctg acggcggacc cgcagatcat cgacgacgcg acggtcgtcg
      121 ccgactttcg cggcggtcgc ctcgtcgacg gctggggccc ggagggcgag gggctgtggg
      181 cacacgaggt ggtcgagacc agccaggcgg ccgacctcgt cgccgacgcc gagcacatct
      241 acgcgctgat ccggcacacg gcgccgccga gcgcgacgct cgtgtcgctc gtcgaggcga
      301 ccggcctcgg caagccaggc tttggcagcg gcggggtgcg atcgtacacc gcgccgggcg
      361 gggtgaagat cgacccgagg cagctcggcc tcgccgccga tcgcctgtac atccagggcc
      421 aggtggtcct tgacaccgtg gtgtcggcgg cgacgctcga cagcggccag ccgatcaacg
      481 acttcgggac ccacaagtcg cagtactcga tgcgcggagc gacgccggat ccgtcgagcg
      541 gcacggtgta cgtcggcatc gacgacttcg gcgtcgtcgg ggcggagcgc ctgttcccga
      601 acggcgacgt cgacatgacg tggggcgacg ccgggaagcg gctgctcgcg gcgccgtccg
      661 cctggaagtc gacgtgggat cccggcaagg agctgctggt gctgcgcgag ctcgaggtcg
      721 acagcagcgg ccgactgtac gcgctgctga cgtcgttcga cgaggccaac accggcgagg
      781 tcgcgctggt catgcgcctc gacacgagcg gcgccctcga cccgagcttc gggctcggcg
      841 gcgcggcgct gtgggcgatc gacgaccagc cgggcaccga cccgaagcgg atgcagatca
      901 cggcggacgg cgggatcgtc gtcgtcggcg cggactacgg gcaatggttc gccacgcggc
      961 tgacggcgga cggtgacctc gacctcggct ggggcgacgg cgggcacgta caccacgcgt
     1021 acatgcgaga catcggcagc ccggcgtcga ccgcgggcgt cgacgtcgcg gtgatgccgg
     1081 acggccacgt cgtgttcttg atcaacatcg agccggcctc cgagctgacg gccctggtcc
     1141 gctacacgca ggacgggcag gtcgacctcg acttcggcga ccccgaggcg gacggcgacg
     1201 gctggtcgct gatcgacacc gcacccgtgc gcctcgcgcc cggcgtcgac ggccgtgtct
     1261 tcgtgctcgg tcagttcacg tacagcgaca gtgacgtgac catcagcgac gcgtggatca
     1321 acgcggtcca cacgtactga cgggcgcgct cagcggtcgc gctcgccgtc tcgcggggcg
     1381 gcgccgagcc cagcaggcca tcgtgacaac ctggccacgc tggctttgcc tgcacccgag
     1441 ccgcggcata aagcgcgtgc gatcgacgcg ctcgaactgc gaccggcacc gccgcagggt
     1501 cgcaatcgaa catcgtgcat cgtccagtcg ccgacccgcg ggtccatcga ccggtcgagg
     1561 acgagatcgt gtaagcgcgg cccgcggggc cgcgccgggt tacactcggc cgtgtatcgc
     1621 cgtcgctcgc ggtcatcgcg gcgggaacga aggatgatcg tcatgacgaa gacatcgagc
     1681 ctgtttgtcc ttggcctggg cgcgtcgctg tggggttgca atgggggcgg ctcggccggg
     1741 accgagaccg acggcagcac cggcgtgacg tcgatcgtgc ccacgaaccc gaccacgacg
     1801 ggcacgaccg ggagcaccgc gaccacgtcc ggtccgaccg agccgacagg cccgagcgaa
     1861 ccgaccgcgg gcacaaacga cacgaccgcc gcggcgacgg aggccactgg cgacaccggc
     1921 accaccgaac ccgcggccga cacgaccggc gacaccggca ccaccggacc tgccggcgac
     1981 accaccggcg acaccaccgg cgccgaggag gtctgcgcgg ccccgacgac gtacatcacg
     2041 tgcgacgcgg ccctcgaccc ggccgacccg ctcgcgccgt ttcaggcgat gggcctcggc
     2101 tgtcctggcg acgcgagcga ctcgatcacc atcagcgagc cgtcgttcaa ctcgcagaac
     2161 ccgaacgcct ggcggatcgc caccggcttc ggcagctatc acgaggacaa cgaccccgac
     2221 aagccgctct acttcagccc gcgcgagggc ggctcgttcc tgatcatcag caccggcgtg
     2281 gtcgcccagg ccgatccggc gggcgtggtc gtcgaggccg cgaagtcgca ggacgacaac
     2341 ggcaacaacg gcaacgacga cgccgacgag ctgccgcctc cgttcaacgt cgtgcccggc
     2401 agcaacggcg gcgccggcgg cacgcccttc ctcgactgcg acggcgtcgg cgactgctcc
     2461 gacacgctcc agacccaatg gaacctcggc gaggccaacc ccaacgacaa gctgtggttc
     2521 tcgttcaagg ccacggtgcc gatgggcacc cacggttacg tgttcaactt cgcgtactgc
     2581 tcggccgagt atccgatgta catcgattac aagtacaacg atatggtcgt cgcctggcag
     2641 atcagcgagg cgtacaccgg caacgtcacc ttcgtgaagt acatggacga gca
//
LOCUS       DASWNJ010000422         1792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_360057, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000422 DASWNJ010000000
VERSION     DASWNJ010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360057"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /locus_tag="VGB85_07250"
     CDS             <1..1148
                     /locus_tag="VGB85_07250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0133857.1"
                     /translation="ALATIAASYVSPRVRQHLRLGPLVMCYAMFAYFMYLGYRHDMAL
                     EDILGLLPVVAGIAVLARRASDVIVLLAYITALLLVVYSHLEAPRAHISEPFAIFVVF
                     AGILGWMSMWRTRLEGALQVANATLEARVADRTASLEREVVERVAAERRANAASEAKS
                     RFLANMSHELRTPLNAVIGYTELVEQELADSDQAHLCSDLDRVGRAARHLLTIIDDVL
                     DLSRIEAGSRAFTSETVPVRGIIEDAVVLVQPAIDARRNRLTLTIAPDLLVLGDRAAL
                     VRVLVHLLDNAAKFTTAGEIDVVARGAGPEVAIEIHDTGIGIPSAAISQVFDRFTQAD
                     DSSTRLHGGAGLGLAICKEMVARMHGRITVTSEVGEGSVFTVHLPAL"
     gene            1347..>1792
                     /locus_tag="VGB85_07255"
     CDS             1347..>1792
                     /locus_tag="VGB85_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133858.1"
                     /translation="MPTRPTLGDRFRYAFDGLIARGAWALITWHLLIALATVFAISLL
                     VAVVGQTPVGEDGQPIDFAALYWTVLMHAIDPGTITGDEGDAAWRTLMMIATIAGIIL
                     IGSLVASLVASVTQRFHELRRGHSRVIEEGHTLVLGWSRQIFTIIR"
BASE COUNT          231 a          638 c          644 g          279 t
ORIGIN      
        1 ccgcgctggc gacgatcgcc gcctcgtatg tctccccccg ggtgcgccag cacctgcggc
       61 tcgggccgct ggtgatgtgc tacgcgatgt tcgcctattt catgtacctc ggttatcgcc
      121 acgacatggc gctcgaggac atcctcgggc tgttgccggt ggtcgccggg atcgcggtgc
      181 tcgcccgtcg cgccagcgat gtcatcgtcc tgctcgcgta catcacggcg ctgctgctgg
      241 tggtgtacag ccacctcgag gcgccccggg cccacatcag cgagccgttc gcgatcttcg
      301 tcgtgttcgc ggggatcctc gggtggatga gcatgtggcg gacccggctc gagggggcgc
      361 tgcaggtcgc caacgcgacg ctcgaggcgc gggtcgccga ccgcaccgcg tcgctcgagc
      421 gcgaggtggt cgagcgcgtc gccgccgagc ggcgggccaa cgcggcgagc gaggccaagt
      481 cgcggttctt ggccaacatg agccacgagc tgcggacccc gctcaacgcg gtgatcggct
      541 acaccgagct cgtcgagcag gagctcgccg actccgacca ggcccacctg tgcagcgacc
      601 tcgaccgggt cggccgcgcg gcgcggcacc tgctgacgat catcgacgac gtgctcgacc
      661 tctcgcgcat cgaggccggc tcgcgcgcgt tcaccagcga gacggtcccg gtgcgcggca
      721 tcatcgagga cgcggtcgtg ctggtccagc cggcgatcga cgcacggcgt aatcgcctga
      781 ccctgacgat cgccccggac ctgctggtcc tcggggaccg cgcggcgctg gtccgcgtgc
      841 tcgtgcacct gctcgacaac gcggcgaagt tcacgacggc cggcgagatc gacgtcgtcg
      901 cccgcggggc cggccccgag gtggcgatcg agatccacga taccggcatc ggcatcccga
      961 gcgccgcgat ctcgcaggtg ttcgatagat tcacccaggc ggacgacagc tcgacgcggc
     1021 tgcacggcgg cgccggcctc ggcctggcga tctgcaagga gatggtcgcg cgcatgcacg
     1081 gccggatcac cgtcaccagc gaggtcggcg agggcagcgt gttcacggtc cacctgccgg
     1141 cgctgtgatc ggtgacccgt tttggcgacc cgcggcggcg cgctcgcggc gtgaggcggg
     1201 tgtcgacgcg ccggctcgcc cgaggtgatc cgcggccgtg gcgggcacgg gcgcgatcgt
     1261 tcggcgcgcg gcgggcttgc gggcgcccca ggcgcgtggc gggcccttga gcggcggggc
     1321 cccaggcgcc atgatgcgcg ccccgtatgc ccacgcgacc cacgctcggc gaccgcttcc
     1381 gctacgcctt cgacggcctg atcgcccgtg gtgcgtgggc gctgatcacc tggcacctgt
     1441 tgatcgcgct ggcgacggtg ttcgcgatct cgctgctggt cgccgtcgtc ggtcagacgc
     1501 cggtcggcga ggacggtcag ccgatcgact tcgcggcgct gtactggacc gtgctgatgc
     1561 acgcgatcga ccccggcacg atcaccggcg acgagggcga cgcggcctgg cgcacgctga
     1621 tgatgatcgc gacgatcgcg ggcatcattc tcatcggcag cctcgtcgcc tcgctggtcg
     1681 ccagcgtcac ccagcggttc cacgagctgc gccgcggcca ctcgcgcgtg atcgaggagg
     1741 gtcacacgct ggtgctcggc tggtcgcgcc agatcttcac gatcatccgc ga
//
LOCUS       DASWNJ010000423         3960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_538527, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000423 DASWNJ010000000
VERSION     DASWNJ010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538527"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2180
                     /locus_tag="VGB85_07260"
     CDS             <1..2180
                     /locus_tag="VGB85_07260"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEY0133859.1"
                     /translation="TALPVLERTLGTWPARPLEQPTPAVAEKISKRAASEVVGEGEQA
                     VTIGWSTVPFTHPDEPVLQVMHWLLDNDAAGLLNVELELSQKVPDASAYGYSMLESGV
                     FIARATAREGQPLEEVESLLLGVIAKLKAGDFTQQDIDAIVLANDIQLKMQQESNDGR
                     VSRLMQSYIYRQPWQKMVERDVRLRKVTRDDVIRAAKAYLTDGFSVVYRRAGKPVVAK
                     IEKPKITAIEVDPSRKSKFGAQIEAMKAPELNPEWLVEGTHYVRQTLPAGPMVGVVNK
                     RNDLFSLGYHFDRGYKRDPMLCHALDLLEQSGAGEQSAEAFKRELFALGSTIDFGCSA
                     DGSAIRVQGIDANLEKTLALLDRWMRTPKLDADTLQKLGENVLSTRKDGMEDPDNLWT
                     VLGEYAQFDRESDYLHAPSNKQITSARVEPLARMIQGFLDHQHKTLYYGPRSAADAAK
                     VVALGKSHRKLTHRPPLVYRKTKTPTIYFVHREVAKSYVSVTLPHGPGERARKPTARL
                     FNTYLNGGMNALLFQEMREARGLVYFSYGSVDQGRYPDDPWALTGGMGTQADKTPDAL
                     KTFLELVKTRPLDPGRISVARTTIDQEFRSSRIDPRYSLYWIDSWDDAGEPSDPRPWY
                     WEQIQALPEEPLRNFASSFADKPVIIGIIGDRNRIDMAALEQVGKVIELTPDKLFSYG
                     PFPKPKAAAAPIAAEPAAPADKPTTAAAPAKKPPPPPPAPAAN"
     gene            complement(2311..2697)
                     /locus_tag="VGB85_07265"
     CDS             complement(2311..2697)
                     /locus_tag="VGB85_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133860.1"
                     /translation="MRQYAVILIAVVVLIAGFVARRYLAPGQTASTAVPAEVEAATRA
                     LNAADALVLTLASGQYAVTELDPKVRAMRESCEALENLRPTAACSELRSKGDALQRAA
                     RNQTIGESAAQDFRAATATLRAELGR"
     gene            complement(2737..3897)
                     /locus_tag="VGB85_07270"
     CDS             complement(2737..3897)
                     /locus_tag="VGB85_07270"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEY0133861.1"
                     /translation="MHRTRALAPATLAIHAGTPGPQPAGAVVQPIYQSSTFLYAGQQS
                     YHDVPYIRLNNTPNQSALARTLAALEGGEAALVSASGMAAISTTLLTVLAAGDHLLAQ
                     RRLYGGTHSLLVHDLPRLGITVDFIDPDRPETWAAALRSNTRALYSETLTNPLLRLAD
                     LPAMAAFARAHGLVSMIDNTVATPVHFRPIEHGFDLVLHSATKYLNGHSDIVAGAVVG
                     SAALVEQVRQRQNHLGGALDPHACFLLQRGLKTLTLRMRQHSASAIAIAEFLARHPAV
                     ARVNYPGLAQHPDHARARALLPTGCSGLVSFELAGGRPAAEALLARVTLPAVAASLGG
                     AESLITIPATTSHAALSVSEREAAGIAEGLVRLSVGLEDTQDLLDDLALALA"
BASE COUNT          629 a         1500 c         1321 g          510 t
ORIGIN      
        1 cgaccgccct gccggtgctc gagcgcaccc tcgggacctg gcccgcgcgc ccgctcgagc
       61 agccgacccc cgccgtcgcc gagaagatca gcaagcgcgc cgcctccgag gtcgtcggcg
      121 agggcgagca ggcggtcacg atcggctggt cgaccgtgcc cttcacccac cccgacgaac
      181 ctgtccttca ggtcatgcac tggctgctcg acaacgacgc cgccggcctg ctcaacgtcg
      241 agctcgagct cagccagaag gtccccgacg ccagcgccta cggctacagc atgctcgagt
      301 ccggcgtgtt catcgcccgc gcgaccgccc gcgagggcca gccgctcgag gaggtcgagt
      361 cgctgctgct cggcgtgatc gccaagctca aggccggcga cttcacgcag caggacatcg
      421 acgcgatcgt gctcgccaac gacatccagc tcaagatgca gcaggagagc aacgacggcc
      481 gcgtctcgcg gctgatgcag tcgtacatct atcgccagcc gtggcaaaag atggtcgagc
      541 gcgacgtccg gctgcgcaag gtcacccgcg acgacgtcat ccgcgccgcg aaggcctacc
      601 tgaccgacgg ctttagcgtc gtgtaccgcc gcgccggcaa gccggtcgtc gccaagatcg
      661 agaagccgaa gatcaccgcg atcgaggtcg acccgagccg caagagcaag ttcggcgccc
      721 agatcgaggc gatgaaggcc ccggagctca acccggagtg gctcgtcgag ggcacccact
      781 acgtgcgcca gaccctgccc gccggcccga tggtcggcgt cgtcaacaag cgcaacgacc
      841 tgttctcgct cggctaccac ttcgaccgcg gctacaagcg cgacccgatg ctgtgccacg
      901 cgctcgacct gctcgagcag tccggcgccg gcgagcagtc ggccgaggcg ttcaagcgcg
      961 agctgttcgc cctcggcagc acgatcgact tcggctgcag cgccgacggc agcgcgatcc
     1021 gggtccaggg catcgacgcc aacctcgaga agaccctcgc cctgctcgat cgctggatgc
     1081 ggacgccgaa gctcgacgcc gacaccctgc aaaagctcgg cgagaacgtg ctcagcaccc
     1141 gcaaggacgg catggaggac cccgacaacc tgtggaccgt gctcggcgag tacgcccagt
     1201 tcgaccgcga gagcgactac ctccacgccc cgagcaacaa gcagatcacg agcgcgcgcg
     1261 tcgagccgct cgcccggatg atccagggct tcctcgacca ccagcacaag acgctctact
     1321 acggcccgcg ctcggcggcc gacgccgcca aggtggtcgc gctcggcaag tcgcaccgca
     1381 agttgaccca ccgcccgccg ctcgtctacc gcaagaccaa gacgccgacg atctacttcg
     1441 tgcaccgcga ggtcgccaag tcgtacgtct ccgtcaccct gccccacggc cccggcgagc
     1501 gcgcccgcaa gccgaccgcc cgcctgttca acacctatct caatggtggc atgaacgcgc
     1561 tgctgttcca ggagatgcgc gaggcccggg gcctcgtcta cttctcgtac ggctccgtcg
     1621 accagggccg ctaccccgac gacccgtggg cgctgaccgg cggcatgggc acccaggccg
     1681 acaagacccc cgacgcgctc aagaccttcc tcgagctggt caagacccgc ccgctcgacc
     1741 ccggccggat ctccgtcgcc cgcaccacga tcgaccagga gtttcgctcg agccgcatcg
     1801 acccgcgcta cagcctgtac tggatcgact cctgggacga cgccggcgag ccgagcgacc
     1861 cgcgcccgtg gtactgggag cagatccagg ccctgccgga ggagccgctg cgtaacttcg
     1921 ccagcagctt cgccgacaag cccgtcatca tcggcatcat cggcgaccgc aaccgcatcg
     1981 acatggcggc gctcgagcag gtcggcaagg tgatcgagct gacgcccgac aagctgttca
     2041 gctacgggcc gttcccgaag ccgaaggccg ccgccgcgcc gatcgccgcc gaaccggccg
     2101 cgcccgccga caagccgacg accgcggccg cgcccgccaa gaagccgccg ccgcctccgc
     2161 ccgccccggc cgcgaactga ggcggcgcag cgtccacacc gtggtcacgc aggagaccac
     2221 gggccggtcg tcgcacggct gctcgcggcg tcgccggcat gtcggcgtcg ggagctaccg
     2281 ggtcgtccgc acccacggcg ccccggtggc tcagcgtccg agctcggcgc gcagcgtcgc
     2341 cgtcgccgcg cgaaagtcct gggccgccga ctcgccgatc gtctgattac gcgcggcccg
     2401 ctgcagcgcg tcgcccttgc tgcgcagctc gctgcaggcc gcggtcgggc gtaggttctc
     2461 gagcgcctcg cagctctcgc gcatcgccct caccttcggg tcgagctcgg tcaccgcgta
     2521 ctgccccgag gcgagcgtca gcaccagcgc gtcggcggcg ttgagcgccc gcgtggccgc
     2581 ctcgacctcg gccggcaccg ccgtgctcgc cgtctgtccg ggcgccaggt acctgcgggc
     2641 gacgaagccc gcgatgagca cgaccacggc gatcaggatc acggcgtact ggcgcatgcc
     2701 cacagtgaac acccgcgcgg cgcccggctc aagggctcac gcgagcgcga gcgcgaggtc
     2761 gtcgagcagg tcctgcgtgt cctcgaggcc gaccgacagc cgcacgaggc cctcggcgat
     2821 gcccgcggcc tcgcgctcgc tcaccgacag cgccgcgtgc gaggtcgtcg ccgggatcgt
     2881 gatcagggac tccgcgccgc cgaggctcgc cgcgaccgcc ggcagcgtca cccgggccag
     2941 cagggcctcc gccgcaggcc gcccgcccgc gagctcgaag ctcaccaggc cgctgcaacc
     3001 ggtcggcagc agcgcccgcg cccgcgcgtg gtccgggtgc tgcgccaggc cggggtagtt
     3061 gacgcgagcg accgccgggt ggcgcgcgag aaactccgcg atcgcgatcg cgctcgcgct
     3121 gtgctggcgc atccgcagcg tcaacgtctt gagcccgcgc tgcagcagga agcaggcgtg
     3181 cgggtcgagg gcgccgccga ggtggttctg gcgctggcgc acctgctcga ccagcgccgc
     3241 cgacccgacc accgcgcccg cgacgatgtc gctgtgcccg ttgaggtact tggtcgcgct
     3301 gtgcagcacg aggtcgaagc cgtgctcgat cggccggaag tgcaccggcg tggcgaccgt
     3361 gttgtcgatc atcgagacca gcccgtgcgc ccgcgcgaac gcggccatcg ccggcagatc
     3421 ggcgaggcgc agcagcgggt tggtcagcgt ctcgctgtac agcgcgcgcg tgttggatcg
     3481 cagcgccgcg gcccacgtct cgggccgatc gggatcgatg aagtccacag tgatgccgag
     3541 gcgcggcagg tcgtggacca gcaagctgtg ggtcccgcca tacagccgcc gctgcgcgag
     3601 caggtgatcc ccggcggcga gcacggtcag cagcgtcgtg ctgatcgccg ccatcccgct
     3661 cgccgagacc agcgccgcct caccaccctc gagcgccgcg agcgtccgcg ccagcgcgct
     3721 ctggttgggc gtgttgttga gccggatgta aggcacgtcg tggtaagact gctgcccggc
     3781 gtagagaaag gtcgaagatt gatagatcgg ctggaccacc gcgccggccg gctgaggtcc
     3841 cggcgtaccg gcatgaatcg cgagcgtggc gggtgcgagg gccctggtcc tgtgcatgtc
     3901 cgcgagggta cgcgcacgcg cacgcgcgaa caatgccggc gtccgatcag ccctgcaccg
//
LOCUS       DASWNJ010000424         1821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_643494, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000424 DASWNJ010000000
VERSION     DASWNJ010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643494"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VGB85_07275"
     CDS             <1..235
                     /locus_tag="VGB85_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133862.1"
                     /translation="LEIGLDALPGRAVVADLVYVPLETGLLAAARARGNLAVDGLGML
                     LHQAVPGFAHWAGVTPSADGAARARVIEALARR"
     gene            complement(780..>1821)
                     /locus_tag="VGB85_07280"
     CDS             complement(780..>1821)
                     /locus_tag="VGB85_07280"
                     /inference="COORDINATES: protein motif:HMM:NF021637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEY0133863.1"
                     /translation="PWMRPSARVPSLPAGARALPADRHAATPAPAAPPDSVHRPHPPR
                     GVAAVTSKQVDLFLDGLINAPIKDDRALMEFPFFSLQKAPRMRPLVYDDGKIRIEVRP
                     GDRGIATIWDKDVLIYVASIINDRLERGLPVERTIRFNAHNLLQVTGRGSGKRAYELL
                     LDAMFRLRSTTIVTSIEAGDVRERRGFGWIETFRIIERRTAAGKKVMAACEITLNDWM
                     FRAIAKDRRVLTINPGYFDLGMGLKRRLYELARKHCGYQERWVITLPKLIEKCGSVLE
                     PRFFKPQLKRVVTEDDLPDYHVAMGFDPAERPAGAAGDGLDVRRWASNERILITFWPR
                     AAAPLELLAPAG"
BASE COUNT          254 a          584 c          692 g          291 t
ORIGIN      
        1 gctcgagatc gggctcgacg cgctgccggg ccgcgccgtg gtggccgacc tcgtctatgt
       61 cccgctcgag accggcctgc tggccgcggc acgggcgcgc ggcaacctcg cggtcgacgg
      121 gctcgggatg ctgctgcacc aagccgtgcc gggcttcgcc cactgggccg gcgtgacgcc
      181 gtctgccgac ggtgcggcgc gggcccgtgt gatcgaggcc ctggcgcggc gctgagcctc
      241 cggccgcggt gtttggtccg accgcggcgt gaattctcct tcctcccgcc gggtctcgtc
      301 cgcggcgtct cctggaggcc gcggtcgtgg aggcggaccc ctgctggaag cctcttttcg
      361 gccctcgtgc aggcttcgcc ggccccgggt tatccacagg cggcggggcc gccgaatccc
      421 gttaaagagt tagagattct tagttagaga gttagagggc ggaatccgcc ttctggctca
      481 atgctttatc ctgtggattg tgaatgatgt accgaggatg tgtgaacgat gtaccgaatc
      541 aaaccgtgaa cgatgtaccg agtctttgtg aacgatgtac cgagtcgcgg gccacgccgg
      601 gcgtggggaa tgtgaacgat gtaccgagtc ggggcgacgc gccgggagga ggcggagggc
      661 gatgtgaacg atgtaccgag tcggggccgt cccgcggcgc cgggcggcgg gggaatgtga
      721 acgatgtacc gagtcggggc ggcgcgcggc gggcgggcgg gggtgggcga tgtaccgagt
      781 cagccggccg gggccaggag ctcgagcggg gccgccgcgc gcggccagaa ggtgatcagg
      841 atccgctcgt tgctggccca gcggcgcacg tcgaggccgt cccccgcggc ccccgcgggc
      901 cgctcggccg ggtcgaagcc catggcgacg tggtagtccg gcaggtcgtc ctccgtgacc
      961 acccgcttga gctgcggctt gaagaaccgg ggctcgagca cggagccgca cttctcgatc
     1021 agcttgggca gggtgatcac ccagcgctcc tggtagccgc agtgcttgcg ggccagctcg
     1081 tagagccggc gcttgaggcc catgccgagg tcgaagtagc ccgggttgat ggtcaggacg
     1141 cggcggtcct tcgcgatcgc gcggaacatc cagtcgttga gcgtgatctc gcaggccgcc
     1201 atcaccttct tgccggccgc ggtccggcgc tcgatgatcc ggaaggtctc gatccagccg
     1261 aagccgcgcc gctcccgcac gtccccggcc tcgatcgagg tcacgatcgt ggtcgagcgc
     1321 agccggaaca tcgcgtcgag cagcagctcg taggcccgct tgcccgagcc gcggccggtg
     1381 acctggagca ggttgtgggc gttgaagcgg atcgtgcgct cgacgggcag gccgcgctcc
     1441 agccggtcgt tgatgatcga ggcgacgtag atcagcacgt ccttgtccca gatcgtggcg
     1501 atgccgcggt cgccgggcct gacctcgatc cggatcttgc cgtcgtcgta gaccagcggc
     1561 cgcatccgcg gcgccttctg cagcgagaag aacgggaact ccatcagcgc ccggtcgtcc
     1621 ttgatcggcg cgttgatcag cccgtcgagg aacaggtcga cctgcttgct cgtcaccgcc
     1681 gccacgcccc gaggcggatg tggacgatgt accgagtcgg gcggggcggc gggggcgggg
     1741 gtggcggcat ggcggtcggc cggcaaggcg cgggctccgg cgggcaggga ggggacgcgg
     1801 gccgacggac gcatccacgg a
//
LOCUS       DASWNJ010000425         1719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_43213, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000425 DASWNJ010000000
VERSION     DASWNJ010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43213"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VGB85_07285"
     CDS             complement(<1..596)
                     /locus_tag="VGB85_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133864.1"
                     /translation="MLLTIVAITLLALFDERSSARRARGDMLREEGILAEVLAAEFRA
                     GVATLDAGPPMPGTSVEVDGPRWRLRAPDGAALEIEPQQLVHGHMFLESGDHRLLVWI
                     GGTTGLRTLQGQMVDLPRIVRAAEDGLTGAELEPDEAALLGLPRDVAMAGLARVDTRR
                     LGRWTIIVVSSGQHQHEREERQALRMVVDVGLVSLVVLG"
     gene            890..>1719
                     /locus_tag="VGB85_07290"
     CDS             890..>1719
                     /locus_tag="VGB85_07290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0133865.1"
                     /translation="MPRISALLSVLVALCMWACQPVHEKHEEAPVFAVTTPMRQDVEL
                     THEYVAQVNAIQHIEVRAQERGYLTEIFVDEGQLVEEGTKMFQIMPLIYRADLHRAEA
                     EAEMASIEYKNTQMLAEKDIVSAAQLALAAAKRSRARAELELAQTHMRLTEIRAPFTG
                     LMDRFVVRLGSLVDEGDLLTTLSDNRMLWIYFNVPEAEYLDYKADPDHKLAVKFKMAN
                     NKMYDQEGKVETIEADFDSNTGNIAFRATFPNPTGLLRHGETGNIIVATKYPNAQLIP
                     "
BASE COUNT          309 a          595 c          588 g          227 t
ORIGIN      
        1 ccgagcacga cgaggctgac gaggccgacg tcgacgacca tgcgcagcgc ctgtcgctcc
       61 tcgcgctcgt gctggtgctg tccggagctg acgacgatga tcgtccagcg accgagccgc
      121 cgggtgtcga cgcgcgccag cccggccatc gcgacgtcgc gcggcagccc gagcaacgcc
      181 gcctcgtcgg gctcgagctc ggcgccggtg aggccgtcct cggcggcccg gacgatgcgc
      241 ggcaggtcga ccatctgacc ctgcaaggtc cgcaagccgg tcgtgccgcc gatccacacc
      301 agcaggcggt ggtcgccgct ctcgaggaac atgtggccgt gcacgagctg ctgcggctcg
      361 atctcgaggg ccgcgccgtc cggcgcgcgc aggcgccagc gcggcccgtc gacctcgacc
      421 gacgtgcccg gcatcggcgg gccggcgtcg agcgtggcga ccccggcgcg aaactcggcg
      481 gccagcacct cggccaggat gccctcctcg cgcagcatgt cgccgcgggc ccgacgcgcc
      541 gagctgcgct cgtcgaacag cgcgagcaac gtgatcgcca cgatcgtcag cagcatcgca
      601 agcgcgagcg cccaggtacg agcgcgccgc gcttggagga aaggacccat gccgggcgat
      661 gaaagcaaga accgtgacag cggagaagcc ccgcgcttct cgcccgcgcg gaacggatca
      721 gggagcggct ccccgggtac ccagggaaaa attccctggc ggcccccgag gccggggaaa
      781 atggccctta aagcccgcag gaacaacgtc cgggcggttg gtacgggcat tgcttggggg
      841 cggcccggga gcggcgaccc gactggtcgg cgctccggag aatttttcca tgccgcgaat
      901 ttcagcgttg ttgagcgtgc ttgtcgccct gtgtatgtgg gcgtgccagc cggtgcacga
      961 gaagcacgag gaggcgccgg tcttcgccgt gacgaccccg atgcgccagg acgtcgagtt
     1021 gacccacgag tacgtcgcgc aggtcaacgc gatccagcac atcgaggtgc gcgcccagga
     1081 gcgcggctat ctgacggaga tcttcgtcga cgagggccag ctcgtcgagg agggcaccaa
     1141 gatgtttcag atcatgccgc tgatctaccg ggcggacctg caccgcgccg aggcggaggc
     1201 cgagatggcc tcgatcgaat acaagaacac gcagatgctg gccgagaagg acatcgtgtc
     1261 cgcggcccag ctcgcgctgg cggcggccaa gcgtagtcgt gccagggccg agctcgagct
     1321 cgcccagacc cacatgcgcc tgacggagat ccgggccccg ttcaccgggc tgatggaccg
     1381 cttcgtcgtg cggctcggca gcctcgtcga cgagggcgac ctgctgacga cgctgtcgga
     1441 caaccggatg ctgtggatct acttcaacgt gcccgaggcc gagtacctcg actacaaggc
     1501 ggaccccgac cacaagctgg ccgtcaagtt caagatggcc aacaacaaga tgtacgacca
     1561 ggagggcaag gtcgagacga tcgaggccga cttcgacagc aacaccggca acatcgcctt
     1621 tcgcgcgacc ttcccgaacc cgaccgggct gctgcgccac ggcgagaccg ggaacatcat
     1681 cgtcgcgacc aagtacccca acgcccagct gatcccgca
//
LOCUS       DASWNJ010000426         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_290826, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000426 DASWNJ010000000
VERSION     DASWNJ010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290826"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /locus_tag="VGB85_07295"
     CDS             <1..1252
                     /locus_tag="VGB85_07295"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0133866.1"
                     /translation="EPEPTYESIFEHIRAPFPAVAEPPVQGRQTAISLNGAIRAGMQA
                     ILRDNPMTWLYGQDVGKKGGVMVATKGLYERFPEQVRDAPINEPLIIGSAFGFALHPG
                     ATAIPEIQFSDYSLNCLHWLVLLGNQLWQSAGRVNVNVILRLPVEPLHGGSVYHSMCM
                     EGFYGAIPGLTIIAPTTSRDVYGMLRSAADYSGPVVIFESKGLYRMTLGDAFPNEPTD
                     PKEIAALKRAIGFGGHIPDLPDDFRIPLGKAALRRAGKDITIVTWGRCTLYCNEAVAR
                     LVQEGVDCELIDMRTIVPPDMEAVLASVRKTGRLLVVHEDRVFSSLGREIQGAVHEAM
                     RGEHVLSRVLGQDAVPGIPSPVHVEEQIVVSPEKVHRAVQELMAQRRATEIVTPAPVA
                     APVPARAAFTKPQILWTPSRNFVT"
     gene            1254..2096
                     /locus_tag="VGB85_07300"
     CDS             1254..2096
                     /locus_tag="VGB85_07300"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0133867.1"
                     /translation="MSEVAVLDELRVGGLTLTGVTRGGVETCVMVQELGLMFDVGMCP
                     PGSMSYQTILVSHGHADHLGGLPYLVSQHGLMSAPPPTVHMPEEIVAPIRQILDCWSA
                     IEGFDLQADLHGHAPGAHIKVGRDLMARAFRTRHRVPSLGWVVERTTNRLRPEFVGRP
                     PAEIATLRAAGEVITDRHVEPILCVSGDTQIEFFDENELVRRTRVLVHEVTSWDARRD
                     PEQTRRWGHTHLDEVVARAEQFTGEALVLVHRSMRHSRAEAEALVKQRFPASMQGRVH
                     VFGR"
BASE COUNT          335 a          826 c          755 g          340 t
ORIGIN      
        1 cgagcccgag ccgacctacg agtcgatctt cgagcacatc cgggccccct tcccggccgt
       61 cgccgagccg ccggtgcagg gccgccagac ggcgatctcg ctcaacggcg cgatccgtgc
      121 cggcatgcag gcgatcctgc gcgacaatcc aatgacctgg ctctacggac aggatgtcgg
      181 caaaaagggc ggcgtgatgg tcgccaccaa gggcctgtac gagcgcttcc cggagcaggt
      241 ccgcgacgcg ccgatcaacg agccgctgat catcggctcg gccttcggct tcgcgctgca
      301 ccccggcgcg accgcgatcc ccgagatcca gttctccgac tacagcctca actgcctgca
      361 ctggctggtc ctgctcggga atcagctgtg gcagtccgcc ggccgcgtca acgtcaacgt
      421 gatcctgcgg ttgcccgtag agccgctgca cggcggctcg gtctaccact cgatgtgcat
      481 ggagggcttc tacggggcga tccccggcct gacgatcatc gccccgacca cctcgcgcga
      541 cgtctatggc atgctgcgct cggccgccga ctactcgggt ccggtcgtca tcttcgagtc
      601 gaagggcctg taccgcatga ccctcggcga cgcgttcccc aacgagccga ccgaccccaa
      661 ggagatcgcc gcgctcaagc gcgcgatcgg cttcggcggc cacatccccg acctgcccga
      721 cgattttcgc atcccgctcg gcaaggcggc cctgcgccgg gccggcaagg acatcacgat
      781 cgtcacctgg ggccgctgca cgctgtactg caacgaggcc gtcgcccgcc tcgtgcagga
      841 gggcgtcgac tgcgagctga tcgacatgcg cacgatcgtc ccgcccgaca tggaggccgt
      901 gctcgcctcg gtgcgcaaga ccggtcgcct gttggtcgtc cacgaggacc gcgtgttctc
      961 ctcgctcggc cgcgagatcc agggcgcggt gcacgaggcg atgcgcggcg agcacgtgct
     1021 ctcgcgcgtg ctcggccagg acgcggtccc cggcatcccc tcgccggtcc acgtcgagga
     1081 gcagatcgtc gtcagccccg aaaaggtcca ccgcgccgtc caggagctga tggcccagcg
     1141 ccgcgcgacc gagatcgtga cgccggcccc ggtagcagct ccggtgcccg cccgcgcggc
     1201 cttcaccaag ccgcagatcc tgtggacccc gagccgcaac ttcgtgacct gatatgtcgg
     1261 aagtcgcggt cctggacgag ctccgcgtcg gcggcctgac gctcaccggc gtcacccgcg
     1321 gcggcgtcga gacctgcgtg atggtgcagg agctcggcct gatgttcgac gtcggcatgt
     1381 gcccgccggg ctcgatgagc taccagacga tcctcgtgtc gcacggccac gccgaccacc
     1441 tcggcgggct gccctacctg gtctcgcagc acggcctgat gagcgcgccg ccgccgacgg
     1501 tgcacatgcc cgaggagatc gtcgcgccga tccggcagat cctcgactgc tggtcggcga
     1561 tcgaaggctt cgacctccag gccgacctgc acggccacgc cccgggcgcc cacatcaagg
     1621 tcggccgcga cctgatggcc cgggcgtttc gcacgcgcca ccgggtaccg tcgctcggct
     1681 gggtcgtcga gcgcaccacc aaccgcctgc gccccgaatt cgtcggccgc ccgccggccg
     1741 agatcgcgac cctgcgcgcc gcgggcgagg tgatcacgga ccgccacgtc gagccgatcc
     1801 tgtgcgtcag cggtgacacg cagatcgaat ttttcgatga gaacgagctg gtccgccgca
     1861 cccgcgtgct cgtccacgag gtcaccagct gggacgcccg ccgcgacccc gagcagaccc
     1921 gcaggtgggg tcacacccac ctcgacgagg tcgtcgcccg cgccgaacag tttacgggcg
     1981 aggccctcgt cctcgtccac cgcagcatgc gacacagccg cgccgaggcc gaggcgctgg
     2041 tcaaacaacg cttccccgcc agcatgcagg ggcgtgtcca cgtgttcggt cgctgatcgc
     2101 acggtcgtat gatggaagga tcctcggaca gacgggcttg ttcgggggat cggggccgtt
     2161 tgggatggcg ggtggctcgt tcggggcgcc gggctcgtca ggggtgcctg gtggctcgtt
     2221 cggagagcgc ggggcgagga tatctgggcc gggggg
//
LOCUS       DASWNJ010000427         1978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_432095, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000427 DASWNJ010000000
VERSION     DASWNJ010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432095"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VGB85_07305"
     CDS             <1..458
                     /locus_tag="VGB85_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133868.1"
                     /translation="LAGGLTFVPLAVALGWCVAALNEDDLTFWRRAAASAPMLPKLAD
                     NPRLPRGAPTGFAVHTTFDTCDDEDCSAYYFVALAAGDVLRVAVGDGDLRVTETARRH
                     VGAWYDPTYGWQATDLAAGAPYTGWYRVKLTTTKLRGPEPIVTWDASPR"
     gene            634..1812
                     /locus_tag="VGB85_07310"
     CDS             634..1812
                     /locus_tag="VGB85_07310"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133869.1"
                     /translation="MISNPLRCSLLALALAPTACTFPVKAGDDINDPADEDPSPPGDV
                     PTGGPAEPGFCGDGIVGDGEACDDGNDDPADACDSACQPTGAVEWTYRTGSPWTANAV
                     AVGPAGRIVVVGDGEVLALEPDGQEAWLRVMPPDSHAFNSAEIDELGTIYVAGEVGTM
                     HALAPDGDELWRSDVGQNYRAIALGHGALYSVDSDVAGPFEKFIVRRHDRATGAVVWT
                     TESPKGIDVFAHRMAVTGPRVVVVGAVWSPEGDPTLPLVSSFDESGALQAFELGDAEE
                     LTWEGVAATPNGDLALVGHASDIVVARVGADLSPQWALADEQGSAWTISSGAQGELAI
                     VGANASGSFVRRLDAAGATVWTNFYGPVGAPSAVEFGPGFLVAIGNEEGGIWVRRISD
                     "
BASE COUNT          266 a          707 c          722 g          283 t
ORIGIN      
        1 agctcgccgg cggtctgacg ttcgtcccgc tggccgtcgc gctcggctgg tgcgtcgccg
       61 cgttgaacga ggacgacctg accttctggc gccgcgccgc cgcgtcggcg ccgatgctgc
      121 cgaagctcgc cgacaatccg aggctgccac gcggcgcgcc gaccgggttc gccgtacaca
      181 cgaccttcga cacctgcgac gacgaagatt gctcggcgta ttacttcgtc gcgctggcgg
      241 ccggcgacgt gctgcgcgtc gcggtcgggg acggcgacct gcgcgtgacc gagacggcga
      301 ggcggcacgt cggtgcgtgg tacgacccga cctacgggtg gcaagcgacg gacctcgcgg
      361 ccggcgcccc gtataccggg tggtaccggg ttaagctgac caccacgaag ctccgcggcc
      421 ccgagccgat cgtcacctgg gacgcgtcgc cgcggtgagc ctgcgcctcg agcttcgcgt
      481 cggtggccgc gcgcagatcc gcgctgacgg cgattcgccg ctcgcgcgaa actacgaaca
      541 ttgcgtatgt gcgccgcgat cgacgatctt tcgcggtcgt gacggaacgc gggcgacccg
      601 gcgatcgacc cctgtggagg caaacatccc ttggtgatct cgaacccgct gcgctgttcc
      661 ctgctcgccc tcgccctcgc accgaccgcg tgcaccttcc cggtcaaggc gggtgacgac
      721 atcaacgatc ccgccgatga agatccgtcg ccgccgggcg acgtcccgac cggcggaccg
      781 gcggagccgg gcttttgcgg cgacggtatc gtcggcgacg gtgaggcctg cgacgacggc
      841 aacgacgatc ccgccgacgc ctgtgactcc gcctgtcagc cgaccggcgc ggtcgagtgg
      901 acctacagga ccggctcgcc gtggactgcc aacgcggtcg ccgtcggccc ggcgggccgc
      961 atcgtcgtcg tcggtgacgg cgaggtgctc gcgctcgaac cggacggcca ggaggcgtgg
     1021 ctccgggtga tgccgccgga ctcgcacgcc ttcaacagcg ccgagatcga cgagctgggc
     1081 acgatctacg tcgccggtga ggtcggcacg atgcacgcgc tggcccccga cggcgacgag
     1141 ctgtggcgca gcgacgtcgg ccagaattac cgggcgatcg ccctcggcca cggcgcgctg
     1201 tactcggtcg actcggacgt cgccgggccg ttcgagaagt tcatcgtccg ccgtcacgac
     1261 cgggccaccg gcgcggtcgt gtggaccacc gagtcgccca agggcatcga cgtgttcgcc
     1321 caccgcatgg cggtgaccgg cccgagggtc gtcgtcgtcg gcgccgtgtg gtcccccgag
     1381 ggcgacccca cgctgccgct ggtgagcagc ttcgacgagt cgggcgcgct ccaggcgttc
     1441 gagctcggcg acgcggagga gctgacctgg gagggcgtcg cggcgacccc gaacggcgac
     1501 ctcgcgctcg tcggtcacgc gtcggacatc gtcgtcgcgc gcgtcggcgc cgacctctcg
     1561 ccgcagtggg ccctcgccga cgagcagggc tccgcctgga cgatctccag cggcgcgcag
     1621 ggcgagctgg cgatcgtcgg cgccaacgcg tccggctcgt tcgtccgccg cctcgacgcg
     1681 gccggcgcga ccgtgtggac caacttctac ggccccgtgg gcgcgccgtc ggcggtcgaa
     1741 ttcgggccgg gcttcctcgt ggcgatcggc aacgaggagg gcggcatctg ggtgcgccgg
     1801 atctccgact gatcacgatg ccgaggtcgc ggcgaacagc tcgctgcggg cggcggcgga
     1861 gatcgccacg acgccggggt gcttgagccg gcgttcggac gagatggcga agaagcgctc
     1921 gacggggccc tcggggcggc cgacgagctt cacgccatat tgccggacga tctcgcgg
//
LOCUS       DASWNJ010000428         2962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_678054, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000428 DASWNJ010000000
VERSION     DASWNJ010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678054"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VGB85_07315"
     CDS             <1..630
                     /locus_tag="VGB85_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY0133870.1"
                     /translation="VEVFYRYLSEKLGKLYTVNDNLYTLIDALMTDGRWDLKFLGMQI
                     MIEGLALGAFGVMRQTSREPLLRELLRYVITDEARHVHYGVVALREYYRTLPPKELRE
                     REDWAFEVAVLMRNRFLVHEFYDEFYGHCMTRKQWDVLIMNSQYMRNFRQTMFRRLVP
                     NLKRINLMSDRIRPHYAALGLLEFENDKAAPELSAGELIRDSEAPPQFN"
     gene            complement(640..2190)
                     /locus_tag="VGB85_07320"
     CDS             complement(640..2190)
                     /locus_tag="VGB85_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133871.1"
                     /translation="MPRWFNTAGPCQVENHYMVPVASRMPDVHGLVERKSYFVVHAPR
                     QIGKTTTLVALAAELTASGKYTAALVSMEVGAPFGDQIELAENAVLGTWRRSLQSDLP
                     KELWPPPWPEAAPGERIGAALTAWAKASQRPLVVFLDEIDALQDDALVSVLRQLRDGY
                     RSRPDSFPSSLALVGLRDIRDYRVRGDEREHLGTASPFNISVESLTLKDFTPAEVAEL
                     FAQHTADTGQAVPAETLARIYHWSRGQPWLVNALGRQLMDVLVTDRAKPMQPADVDAA
                     ARILIERQDTHLDSLSERLREPRVRAIVEPMVGGANPQPLPNDDVRYVLDLGLLRRGS
                     DGTIEVSNPIYKEIIVRDLLVVPRISLPKLQPSWLTPAGKLDAQALLAAFIKFWRRHG
                     EPVMRSAPYHEIAPHLVMMAFLDRVANGGGTVEREYAIGSGRLDLCLRLGGESLAIEL
                     KVWREHEPDPREEGLEQLDAYLAGLGLPVGWLVIFDRRPGLPRLSERTTVEDTRTPGG
                     REVRLIRA"
     gene            complement(2299..>2962)
                     /locus_tag="VGB85_07325"
     CDS             complement(2299..>2962)
                     /locus_tag="VGB85_07325"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY0133872.1"
                     /translation="GPVDAPEPAAPAKGEVAVDFLCSPVNPADLLLLSGNYGHRPVLP
                     ARGGLEGVGRVSALGPGVEHLKIGDRVLLPGNAWCERAVIPAGGLFALPAAAASEQLA
                     MLTVNPPTAWGLLHDYVKLEPGDWVILNAANSGVGASMVALTRRLGLRIVSVVRREDA
                     VAELRQQGSDLVRDPELGELNDLHGRASCHSRTGDDTSDPRGDGSVKTCPKGQVVARY
                     RS"
BASE COUNT          456 a         1054 c         1005 g          447 t
ORIGIN      
        1 gtcgaggtct tctaccgcta ccttagcgag aagctcggca agctctacac cgtcaacgac
       61 aacctctaca ccctgatcga cgcgctgatg accgacggcc gctgggacct caagttcctc
      121 ggcatgcaga tcatgatcga ggggctcgcg ctcggggcct tcggcgtcat gcgccagacc
      181 agccgtgaac cactgctgcg cgagctgctg cgctacgtga tcaccgacga ggcccggcac
      241 gtccactacg gcgtcgtcgc cctgcgcgag tactaccgca cgctcccgcc gaaggagctg
      301 cgcgagcgcg aggactgggc cttcgaggtc gccgtgctga tgcgcaaccg cttcctcgtc
      361 cacgagttct acgacgagtt ctacggccac tgcatgaccc gcaagcagtg ggacgtgctg
      421 atcatgaatt cgcagtacat gcgaaacttc cgccagacca tgtttcgccg gctggtcccg
      481 aacctcaagc gcatcaactt gatgtcggac cgcatccgcc cgcactacgc cgcgctcggc
      541 ctgctcgagt tcgagaacga taaggccgcc cccgagctca gcgccggcga gctgatccgc
      601 gactccgagg ccccgccgca attcaactga gaggaacgct caagcccgga tcaggcggac
      661 ctcgcgtccg ccgggcgtgc gcgtgtcctc gacggtcgtg cgctcggata accgcggcaa
      721 gcctggacgg cggtcgaaga tcaccagcca gccgaccggt agcccgagcc ccgcgaggta
      781 cgcgtcgagc tgctcgagcc cctcctcgcg cgggtcgggc tcgtgctcgc gccagacctt
      841 gagctcgatc gccagcgact cgccgccgag gcgcaggcac aggtcgaggc gcccgctgcc
      901 gatcgcatac tcgcgctcga ccgtgccgcc gccgttggcg acgcggtcga ggaacgccat
      961 catcaccagg tgcggcgcga tctcatggta cggcgccgag cgcatcaccg gctcgccatg
     1021 ccgccgccag aatttaataa acgcggccag cagcgcctgc gcgtcgagct tgccggccgg
     1081 cgtcaaccag ctcggctgca gcttcggcag cgagatccgc gggaccacca gcaggtcgcg
     1141 gacgatgatc tccttataaa tggggttgga gacctcgatc gtgccgtcgc tgccgcggcg
     1201 cagcaggccg aggtcgagca cgtagcggac gtcgtcgttc ggcagcggct gcgggttggc
     1261 cccgccgacc atcggctcga cgatcgctcg cacccgcggc tcacgcaggc gctcgctcag
     1321 gctgtcgagg tgcgtgtcct ggcgctcaat taaaatcctc gccgccgcgt cgacgtccgc
     1381 cggctgcatc ggcttcgccc ggtcggtcac cagcacgtcc atcagctggc gcccaagcgc
     1441 gttgaccagc cacggctgcc cgcgactcca gtggtagatg cgcgccagcg tctccgccgg
     1501 caccgcctgc ccggtgtccg ccgtgtgctg cgcgaacagc tcggcgacct ctgccggcgt
     1561 gaagtccttg agcgtcagcg actcgaccga gatgttgaac gggctcgccg tgccgaggtg
     1621 ctcgcgctcg tcgccgcgga cccggtagtc ccggatgtcg cgcaggccga ccagcgcgag
     1681 cgacgacggg aagctgtcgg ggcggctgcg gtagccgtca cggagctggc gcagcaccga
     1741 taccagcgcg tcgtcctgca gcgcgtcgat ctcgtcgagg aacacgacca ggggccgctg
     1801 cgacgccttt gcccaggccg tcaacgcggc cccgatgcgc tcgcccgggg cggcctcggg
     1861 ccacggcggc ggccacagct ctttcggcag gtcgctttgc agcgaccgcc gccacgtgcc
     1921 gagcaccgcg ttctcggcga gctcgatctg gtcgccgaac ggcgccccga cctccatcga
     1981 taccagcgcc gccgtgtact tgccgctcgc ggtcagctcg gccgccagcg ctaccagggt
     2041 cgtcgtcttg ccgatctgtc gcggcgcgtg gacgacgaag tagctcttgc gctcgacgag
     2101 cccgtgcacg tccggcatgc ggctcgccac gggcaccatg tagtggtttt ccacctggca
     2161 cgggccggcg gtattgaacc agcggggcat ctcgcggcgc agtgtaaccg ccgctcgccg
     2221 ggccgcatcg tgaaagaaac gtatgcgggc ccacgcttac ggcgcgaggc aaagcgagga
     2281 attgcagcct ttgagagttc aagagcgata gcgcgcgacg acctgtcctt tgggacatgt
     2341 ctttacagac ccatccccgc gcgggtccga ggtgtcgtcg ccggtgcggc tatggcaaga
     2401 tgcgcggcca tgaaggtcgt tcagttctcc cagttcgggg tcccgcacga ggtcgctgcc
     2461 ctgctggcgc agctcggcga cggcgtcctc gcggcgcacc acggagacga tccgcaggcc
     2521 gaggcggcgg gtcagcgcga ccatgctggc gccgacgccc gagttggcgg cgttgaggat
     2581 cacccagtca ccgggttcga gcttgacgta gtcgtgcagc aggccccacg cggtcggggg
     2641 gttgacggtc agcatggcga gctgctcgct ggcggccgcg gccggcaagg cgaacaggcc
     2701 gccggccggg atgacggcgc gctcgcacca ggcgttgccg ggcaggagca cacggtcgcc
     2761 gatcttgagg tgctcgacgc cggggccgag cgcgctgacg cgaccgacgc cctcgaggcc
     2821 gccgcgcgcc ggtagcaccg ggcggtggcc gtagttgccg gacagcagga gcaggtccgc
     2881 agggttgacc gggctgcaca ggaagtcgac ggcgacctca cccttcgcgg gcgcggccgg
     2941 ctccggcgcg tcgaccgggc cc
//
LOCUS       DASWNJ010000429         2396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_502266, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000429 DASWNJ010000000
VERSION     DASWNJ010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502266"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VGB85_07330"
     CDS             complement(<1..456)
                     /locus_tag="VGB85_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133873.1"
                     /translation="MVFGAYIAVLLGLALNAPAALWNPEHRAFFLVVGTIGLWRYAWN
                     LLHLLRSAWYRQVTFRRWRRLVDRLEAVAAAEPGDAGEALRVPQVFVVVTSYRIRAET
                     SALVFEAALREAIAYGRPATIVASVVELADQRLVKAVFARLAPPPSVRLV"
     gene            772..1461
                     /locus_tag="VGB85_07335"
     CDS             772..1461
                     /locus_tag="VGB85_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0133874.1"
                     /translation="MASGNTLLIVDDDAALRRELAEQLTLHEGFAVREAGTGAEALEL
                     LAGAEPFAAVLLDVGLPDLDGREVCRRLRAQGFKAPIVMLTAHAAESDTIEGLGSGAN
                     DYVTKPFRLGVLMARLRAQLRQFELSDDAVFQVGPYGFKPAAKMLVRPDQRKIHLTEK
                     ETAILKYLYRVGDRAVPREVLLDEVWGYNAGVTTHTLETHIYRLRQKIEPRPGETTLL
                     VTEPGGYRLMR"
     gene            1558..1953
                     /locus_tag="VGB85_07340"
     CDS             1558..1953
                     /locus_tag="VGB85_07340"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0133875.1"
                     /translation="MAKAIHTMVRVLDLDRSIAFYREAFGLEVADRFDFDGFTLAYLR
                     NAQADFEVELTLNKGRTEPYALGDGYGHVAFCVDDLDAEHRRVTDAGLAPNPIKEFHR
                     DGGLMARFFFVQDPDGYKIEVLQRHGRYR"
     gene            1988..>2396
                     /locus_tag="VGB85_07345"
     CDS             1988..>2396
                     /locus_tag="VGB85_07345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEY0133876.1"
                     /translation="MAKCILNRREFLKSAGTAAASVALVSGVTTIFASNGAWAMSLKA
                     LGPHEAEVLLRMTRQLYPHKMLGDVYYAEVVEAFDAKVAADADLAGLVKAGVAELDAV
                     GGLPWLRLSPGTQLEALEKIETGKFFQAVRGHTV"
BASE COUNT          374 a          834 c          867 g          321 t
ORIGIN      
        1 cacgaggcgc accgagggcg gcggcgccaa gcgggcgaag accgccttca cgagccgctg
       61 gtcggctagc tcgaccaccg aggccacgat cgtggccggc cgcccgtagg cgatcgcctc
      121 gcgcagcgcc gcctcgaaca ccagggccga ggtctcggcg cggatgcggt agctggtgac
      181 cacgacgaag acttgcggca cgcgcagcgc ctcgcccgcg tcgcccggct ccgcggcggc
      241 gaccgcctcg agacggtcga cgagccgccg ccagcgccgg aacgtcactt gacggtacca
      301 agccgagcgc aggagatgga gcaggttcca ggcgtagcgc cacaggccga tggtgccgac
      361 gaccaggaag aaggcccggt gctccggatt ccataaggcc gccggcgcgt tgagcgctag
      421 tcccagcagg acggcgatgt aggcgccgaa gaccacgggc ggccccctcg cgccgccggc
      481 cgggtgcgcc gcgggcggcg gcgctagggc gagcggcgtt cggggtgtcg tcgaggcggg
      541 gtgggggaac tcgcacgctg cgccgtctcc gccggcagtc cgcggccccg gcggtggtgc
      601 cggccgcgcg tggggacgcg cctacccaga ggtgctgtcg acgacaagtc tcgcagattc
      661 cgcctaacag ggccttaatc ccgtcaaggc acgcggtcgc ccttgaccgc gctcgtgtca
      721 cgcacgccat aatcgcacgt cggcgggtcc ggcgacgaac ggggtagggg aatggcgtcg
      781 ggcaacacgc tcctgatcgt cgacgacgac gcggccctgc ggcgcgagct ggcggagcag
      841 ctcacgctgc acgagggctt cgccgtgcgc gaggccggga ccggggcgga ggcgctggag
      901 cttctggccg gggccgagcc cttcgccgcc gtgctgctcg acgtgggcct gcccgacctc
      961 gacgggcgcg aggtctgccg ccgcctgcgc gcgcagggct tcaaggcgcc gatcgtcatg
     1021 cttaccgcgc acgccgccga atcggacacg atcgagggcc tggggagcgg cgccaacgac
     1081 tacgtcacca aacccttccg cctcggcgtc ctgatggccc ggctgcgcgc gcagctgcgc
     1141 cagttcgagc tcagcgacga cgcggtgttc caggtcggcc cctacggttt caagccggcc
     1201 gccaagatgc tcgtccgccc cgaccagcgc aagatccacc tcacggagaa ggagaccgcg
     1261 atcctgaagt acctctaccg cgtgggcgac cgcgcggtgc cgcgcgaggt gctgctcgac
     1321 gaggtctggg gctacaacgc cggcgtcacc acccacacgc tcgagaccca catctaccgg
     1381 ctgcggcaga agatcgagcc ccggcccggc gagacgacgc tgctcgtcac cgagcccggc
     1441 ggctaccgct tgatgcgctg aggccggaca gtcggccgca gccgtcgcgc gccgcggcgg
     1501 gctaccgctc gccggcgggg taggcctagt ctcgctcgac ctggaaggga ggcagacatg
     1561 gccaaggcca tccacacgat ggtgcgggtg ctcgacctcg accgttcgat cgcgttctac
     1621 cgcgaggcgt tcgggctgga ggtcgcggac cggttcgact tcgacggctt caccctcgcc
     1681 tacctcagga acgcgcaggc cgacttcgag gtcgagctga cgctcaacaa ggggcggacc
     1741 gagccctacg cgctcggcga cggctacggg catgtcgcgt tctgcgtcga cgacctggac
     1801 gcggagcacc ggcgcgtcac ggacgcgggc ttggccccga acccgatcaa ggagttccac
     1861 cgcgacggcg ggctcatggc gcggttcttc ttcgtccagg accccgacgg ctacaagatc
     1921 gaggtcttgc agcggcacgg ccgttaccgc tagttggaac tagaaaaaat tcagggaggc
     1981 gtgtgggatg gcgaagtgca tcctcaacag gcgcgagttc ctcaagagcg ccggcaccgc
     2041 cgcggcgagc gtggccctcg tctccggcgt gacgacgatt ttcgcctcaa acggtgcgtg
     2101 ggcgatgtcg ctcaaggcgc taggcccgca cgaggcggag gtgctgctgc ggatgacccg
     2161 gcagctctac ccccacaaga tgctgggcga tgtctactac gccgaggttg tcgaggcgtt
     2221 cgacgcgaag gtcgcggccg acgccgactt ggccgggctg gtcaaggcgg gcgtggccga
     2281 gctcgacgcc gtcggcggcc tgccgtggct gcggctctcc cccgggacac agctggaggc
     2341 gctggagaag atcgagaccg gcaaattctt ccaggccgtg cgcgggcaca cggtcg
//
LOCUS       DASWNJ010000430         1936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_290938, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000430 DASWNJ010000000
VERSION     DASWNJ010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290938"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VGB85_07350"
     CDS             complement(<1..183)
                     /locus_tag="VGB85_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133877.1"
                     /translation="MSATSVSLRTSLSTSSVSLALCVSLAASPLAQAAPPPPPPPPSG
                     SATATADQPPDPNAVSA"
     gene            complement(220..807)
                     /locus_tag="VGB85_07355"
     CDS             complement(220..807)
                     /locus_tag="VGB85_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133878.1"
                     /translation="MTPTMVPEPEPEPEPEPPPTVPVQQEVHYAPMQEAPPPRSNMMP
                     IAIGGGIGVAGIAVALVLLMRGPEEKPAPPVAEVKAAPTPVVAAPVPVQPGQPVLGPN
                     GQPLPGQPVLGPNGLPLPVQPGMPVGTGLPGQPIPGQPGDPNAVAAGGTPTTPATTDK
                     KPAAKKKKPSGGGGSTGTKKAEPTKKKSEVVDPFG"
     gene            complement(924..>1936)
                     /locus_tag="VGB85_07360"
     CDS             complement(924..>1936)
                     /locus_tag="VGB85_07360"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEY0133879.1"
                     /translation="ARNADLAVDCLYPDAAVLGAATVRARFTRLLIDLNRAPDDVSAG
                     LVPDHPAPRPRQPPWATTGGRSPPVHNRGLIWRTAVGNVPIFSGPLPYAELRLRLRRY
                     YDPYHRALEVLLERRLARFGYAILLEAHSMPSSVPGDLVLGTFEGGACSPELEATALA
                     ALSAAGPLGVSLAVRLNDPYRGGELVRKFGRPEHGIHALQLEVNRALYMDEASLQLRP
                     EVSRAASPITLASEDASSRPDDRLATPARRAQIASKSSSPRSTPPSAPPFAQAPGALP
                     LALLHRVRSLVATLAAVSPRLATAVAASGVPCPPANPALVASSRGTAHFGEPLCRRRP
                     RG"
BASE COUNT          271 a          591 c          800 g          274 t
ORIGIN      
        1 ggcggacacg gcgttggggt ccggaggctg atccgcggtc gcggtggcgc tgccgctggg
       61 cggcggcgga ggaggcggcg gagcggcctg cgcgagagga gacgcggcga gcgacacgca
      121 gagggcgaga gaaaccgaag aagtggacag cgaggttcgc agcgagacag aggtggcgga
      181 catgtaaggg gggtctcctg actcagcgtg tggctcagct caaccgaagg ggtcgacgac
      241 ttcgctcttc tttttggtgg gctctgcctt cttcgtgccc gtgctgccgc cgccgccgct
      301 cggcttcttc ttcttggcgg ccggcttctt gtcggtggta gcgggcgtcg tcggcgtgcc
      361 gccggccgcg acggcgttcg gatcgccggg ctggcccggg atcggctggc cggggagacc
      421 ggtcccgacg ggcatgccgg gctgcacggg cagcgggaga ccgttcgggc cgaggaccgg
      481 ctgaccgggc agcggctggc cgttcgggcc gaggaccggc tgaccgggct gcacggggac
      541 cggagccgcc acgacgggcg tcggcgctgc cttgacctcg gcgaccggcg gagcgggctt
      601 ctcctcgggt ccacgcatca gcagcacgag cgcgacggcg atgccggcca cgccgatgcc
      661 gccaccgatc gcgatcggca tcatgttgct gcgtggaggc ggggcctcct gcatcggtgc
      721 gtagtgcacc tcctgctgga ccgggacggt cgggggtggc tcgggctcgg gctcgggctc
      781 gggctccggc accatcgtcg gagtcatggc gagctcgtcg acgagttgcc ctagcaagtc
      841 atcaaacgca tcggcgttgt cgcgttggga tttgggtgct gccacggtga tactccttcg
      901 ggccgaaggt cgcaggatcg atgttagcct cttggccgcc gacgacacaa gggttcgccg
      961 aaatgcgcgg ttccccgcga cgaagcgaca agagcggggt tggcaggggg gcagggcacc
     1021 ccggaggcgg ctacagccgt cgccagacgc ggtgagacgg ccgcgagcgt tgccacgagg
     1081 gatctcacac gatggaggag cgcgaggggc agggcgcccg gcgcctgagc aaatggcggg
     1141 gcagatggcg gagtcgaccg tggactcgaa gatttcgacg cgatctgtgc ccgtcgggcc
     1201 ggcgttgcga ggcgatcgtc gggccgcgag gacgcgtcct ccgacgcaag tgtgatcggg
     1261 ctggcggcgc gagagacctc ggggcgaagc tgcaagctgg cctcgtccat gtataaggcc
     1321 cggttcacct cgagctgcaa cgcgtggatg ccgtgctcgg gtctaccgaa cttgcggacc
     1381 agctcgccgc cgcgataggg gtcattgagg cgtacggcga ggctgacgcc cagcgggccg
     1441 gcggccgaga gcgcagccag ggcggtcgcc tcgagctccg ggctgcacgc gccgccctcg
     1501 aaggtgccga gcacgaggtc gcccgggacg ctgctgggca tgctgtgggc ctcgagcagg
     1561 atcgcgtagc cgaagcgggc caggcgacgc tcgagcagga cctcgagcgc gcggtggtag
     1621 gggtcgtagt atcggcgcag gcgcaggcgc agctcggcgt agggcagcgg gccgctgaag
     1681 atcgggacgt tgccgacggc cgtccgccag atcaggccgc ggttatggac cgggggcgag
     1741 cgaccaccgg tggtcgccca tggcggctgg cgcgggcgtg gggccgggtg gtcggggacc
     1801 aggccggcgc tgacgtcgtc gggggcgcgg ttgaggtcga tcagcaggcg ggtgaagcgc
     1861 gcgcgcacgg tggccgcgcc gagcacggcg gcgtcggggt agaggcagtc gaccgcgagg
     1921 tcggcgttgc gggcga
//
LOCUS       DASWNJ010000431         1834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_184577, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000431 DASWNJ010000000
VERSION     DASWNJ010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184577"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VGB85_07365"
     CDS             <1..576
                     /locus_tag="VGB85_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133880.1"
                     /translation="MGILIGSGDVVIQPPLGYGFGLKLAFYAVRLGPMRFGFAFHGGH
                     TRYMGRRDFTIMTIMSEPGGPAPVVRRWNVLAHTDVALGPHFQVPAGPLLIEFGAGGG
                     LGVSSYTRVVDAEPGHDESTVAYNGLVRADATLGIPIRNNQGIAVGADLQKYFSSTRV
                     VTVPDAPMGAPPDSVVFDLTLAVTLAYQMWF"
     gene            584..>1834
                     /locus_tag="VGB85_07370"
     CDS             584..>1834
                     /locus_tag="VGB85_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEY0133881.1"
                     /translation="MTVTTLSAHPTRRAPEGPVVAIVMDGVGLGLGDLGDAVSQAHTP
                     NLDVLRALPSFTSLLAHGTAVGMPSDADMGNSEVGHNALGAGRILDQGAKLVNEAIAT
                     GAMFIGAAWRDAVNTVLGSGEPLHFIGLLSDGNVHSHIEHLIAMLQRAATEGVKKARV
                     HALVDGRDVPGRSALLYVDRLEAALAELSTDGRDYRIASGGGRMVVTMDRYEADWNIV
                     ARGWAIHVRGEGRRFPNARVAITTLYNETGLDDQNLPGFVIADEQGPIGPIRDGASVL
                     FFNFRGDRALEITRAFDQDVFTGFDRGVRPRVFYAGMTQYDGDLRLPARYLVSPPVIE
                     GTMGELLAYSGASQLAISETHKFGHVTYFWNGNRSEPFDAQLEKYVEIPSDLAPLEQR
                     PWMKAAEITDRLIAELDQMHPRFVR"
BASE COUNT          274 a          695 c          589 g          276 t
ORIGIN      
        1 atgggcatcc tgatcggcag cggcgacgtc gtcatccagc cgccgctcgg ctacggcttt
       61 ggcctcaagc tcgcgtttta cgcggtgcgc ctcggaccga tgcgcttcgg cttcgccttc
      121 cacggcggcc acacgcgcta catgggccgg cgcgacttca ccatcatgac catcatgagc
      181 gagcccggcg gccccgcgcc ggtcgtgcgc cgctggaacg tgctcgcgca caccgatgtc
      241 gccctcggcc cgcacttcca ggtcccggcg ggtccgctgc tgatcgagtt cggggccggc
      301 gggggcctcg gcgtgtcgag ctacacccgc gtcgtcgacg ccgagcccgg ccacgacgag
      361 agcacggtcg cctacaacgg cctggtccgc gccgacgcga ccctcggcat accgatccgc
      421 aataaccagg gtattgcggt cggggccgac ctacaaaaat acttctcgag cacccgcgtc
      481 gtcaccgtgc cggatgcgcc gatgggcgcg cctccggaca gcgtcgtctt cgacctgacg
      541 ctggccgtca ccctcgccta tcagatgtgg ttttaagcaa agcatgaccg tcacgaccct
      601 cagcgcacac ccgacgcgcc gcgcccccga aggcccggtc gtggcgatcg tcatggacgg
      661 cgtcggcctc gggctcggcg acctcggcga cgccgtctcg caggcgcaca cgcccaacct
      721 cgacgtcttg cgcgccttgc cgtcgttcac cagcctgctc gcgcacggca ccgcggtcgg
      781 catgccgtcg gacgccgaca tgggcaacag cgaggtcggc cacaacgcgc tcggcgccgg
      841 ccggatcctc gaccaggggg ccaagctcgt caacgaggcg atcgccaccg gcgcgatgtt
      901 catcggcgcc gcctggcgcg acgcggtcaa caccgtgctc ggctccggcg agcccctgca
      961 cttcatcggc ctgctgtcgg acggcaacgt ccacagtcac atcgagcatt tgatagcgat
     1021 gttgcagcgc gcggccaccg agggcgtcaa gaaggcccgc gtgcatgcct tggtggacgg
     1081 ccgcgacgtc cccggccgct ccgcgctgct ctacgtcgac cgcctcgagg ccgcgctcgc
     1141 cgagctctcg accgacggcc gcgactatcg catcgccagc ggcggcggtc gcatggtcgt
     1201 gacgatggac cgctacgagg ccgactggaa catcgtcgcg cgcggctggg cgatccacgt
     1261 gcgcggcgag ggccgccggt tcccgaatgc ccgcgtggcg atcaccacgc tctacaacga
     1321 gaccggcctc gacgaccaga acctgcccgg cttcgtcatc gccgacgagc agggcccgat
     1381 cgggcccatc cgcgacggcg cgtcggtgct gttcttcaac ttccgcggcg accgggccct
     1441 cgagatcacg cgggccttcg accaggacgt cttcaccggc ttcgatcgcg gcgtgcggcc
     1501 gcgggtgttc tacgccggca tgacccagta cgacggcgac ctgcgcctgc cggcccgcta
     1561 cctcgtgtca ccgccggtga tcgagggcac gatgggcgag ctgctggcgt actccggcgc
     1621 ctcgcagctg gcgatcagcg agacccacaa gttcggccac gtcacttact tctggaacgg
     1681 caaccgctcg gagcccttcg acgcccagct cgagaagtac gtcgagatcc cctcggacct
     1741 cgcgccgctc gagcagcgcc cgtggatgaa ggccgccgag atcaccgacc gcctgatcgc
     1801 cgagctcgac cagatgcatc cccgcttcgt gcgc
//
LOCUS       DASWNJ010000432         2935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_502329, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000432 DASWNJ010000000
VERSION     DASWNJ010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502329"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1010
                     /locus_tag="VGB85_07375"
     CDS             150..1010
                     /locus_tag="VGB85_07375"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0133882.1"
                     /translation="MRRAFFILPALLPACGVEELQAILDKYDTFGESSSSGEVDTTAV
                     GSSTSSGEPGESGTDSTGAPASTSTAGTGTGGLDTTADTTADPGSTGPAPPVCGDGVV
                     EGDEECDDANAVDGDGCYNDCTRSWLVFVTSEPFTKGDIKGLIGADYQCRHRASKLFL
                     PNGDRYMAWISTTEVQPADRMYHARGPYRLVNGDQVAASWDALVTGPLDHPIMLSELG
                     EVVNALVFTGTTPQGWRVPNSTRCDDWTDSAWDNFAWYGDSTATDNLWTLAIESFCGG
                     GAALYCFEQP"
     gene            complement(1051..1692)
                     /locus_tag="VGB85_07380"
     CDS             complement(1051..1692)
                     /locus_tag="VGB85_07380"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEY0133883.1"
                     /translation="MANPADTPELDLVDPAAPAPVGLTGLGLVAHMVFILIGGVLVFF
                     FAASLMPALRSQAAAPCRSLEPSPRTGPAPAFVVQDLAGAPVALADFAGKLVIVNFWA
                     TWCEPCIREWPQLDRLAERFAGRDEVVILAISVDSDPALIQPFLERMSLTSTRVKVLW
                     DPKQDVQKAFGTDKLPDTYFVDRAGQLVHAFINVRDWGRPAAYQCVESMVARR"
     gene            complement(1710..>2935)
                     /locus_tag="VGB85_07385"
     CDS             complement(1710..>2935)
                     /locus_tag="VGB85_07385"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY0133884.1"
                     /translation="QMGPNDALITIVEWSDFQCPYCSRVSEPLKAAMDSYEGDVRLIF
                     KHFPLGGHAQASPAARAAWAAHQQGKFWEMHDQLFANQKKLGDADFTEYARQIGLDVA
                     KFDLDRKSAAASAAVDNDQASGNKAGLSGTPYFLVNGHNYSGALPAHQWKQVFEYELK
                     AAKALVDAGTPRDQVYAELMKDAKATRGGNAGAQPQRARRPGEPDPAKTYKVPVDARP
                     QTGPDDALVTIVEFSDFECPFCSRVNASMDEVKSTYGNDVRVVFRQRPLSFHANARPA
                     AKAALAAHRQGKFWEMHDRLFSNQKALGKADLIGYAQELGLDTGMFELDMEAADIEQM
                     IKDDEALAGKVGVNGTPAFFINGRFLNGAMPFSEFARLVDEGKAAAQKLIDAGTPRAQ
                     VYDKLMETAEEKPGA"
BASE COUNT          440 a         1027 c         1008 g          460 t
ORIGIN      
        1 ctacagctcg ggcggctcgg gcaggtatct cccgtcgccg ccgatgtgcc gacgtggctt
       61 cagaacgcgc actggacgca atacagcgca atctcctgca aatataagta tcacttctac
      121 tgcttcgagc agtgatcccg gagccactca tgcgccgcgc cttcttcatc ctccctgcgc
      181 tcctcccggc atgcggcgtc gaggagttgc aagccatcct cgacaagtac gacaccttcg
      241 gcgagtcgag cagctccggc gaggtcgaca ccaccgcggt cggcagctcg accagcagcg
      301 gcgagcccgg cgagtccggg accgacagca ccggcgcccc cgcctcgacc tccaccgcgg
      361 gcacgggtac gggtggcctt gacaccaccg ccgacaccac cgccgacccc ggcagcacgg
      421 gtcccgcccc gcccgtgtgc ggcgacgggg tcgtcgaggg cgacgaggag tgcgacgacg
      481 ccaacgcggt cgacggcgac ggctgctaca acgactgcac ccgctcctgg ctggtgttcg
      541 tcaccagcga gccgttcacc aagggcgaca tcaagggcct gatcggcgcc gattatcagt
      601 gccgccaccg cgccagcaag ctgttcttgc ccaacggcga tcgctacatg gcgtggatca
      661 gcaccaccga ggtccagccc gccgaccgca tgtatcacgc ccgcggcccc taccgcctcg
      721 tcaacggcga tcaggtggcc gccagctggg acgccctcgt caccggcccg ctcgaccacc
      781 ccatcatgct cagcgagctc ggcgaggtcg tcaatgccct ggtgttcaca ggcaccacgc
      841 cccaggggtg gcgcgtgccc aactcgaccc gctgcgacga ctggaccgac agcgcctggg
      901 acaacttcgc ctggtacggc gactccaccg cgactgacaa cctctggacc ctcgccatcg
      961 aatccttctg cggcggaggg gccgcgctgt attgcttcga gcagccatga gcttgctcgg
     1021 gcgcctcgcg ttcgggcgac ggtctccggc tcagcggcgc gcgaccatcg actcgacgca
     1081 ctgatacgcg gcggggcggc cccagtcgcg cacgttgatg aacgcgtgca cgagctggcc
     1141 ggcgcggtcg acgaagtagg tgtcgggcag cttgtcggtg ccgaacgcct tttgcacgtc
     1201 ctgcttgggg tcccacagca ccttgacccg cgtactggtc aggctcatgc gctcgaggaa
     1261 gggctggatg agcgcggggt cggagtcgac gctgatcgcc aggatgacga cctcgtcgcg
     1321 gccagcgaag cgctcggcga ggcggtcgag ctgcggccac tcgcgaatgc acggctcgca
     1381 ccacgtcgcc cagaagttga cgatcaccag cttgccggcg aagtcggcga gcgccaccgg
     1441 ggcccccgcg aggtcctgca ccacgaacgc cggcgcgggg ccggtgcgcg gcgagggctc
     1501 gagcgagcga cacggcgccg ccgcctgcga gcgcagcgcg ggcatcagcg aggccgcgaa
     1561 gaagaacacc agcacgccgc cgatcaggat aaacaccatg tgcgcgacca ggccgagccc
     1621 cgtcaggccg accggagcgg gcgcggccgg gtcgacgagg tcgagctctg gagtatcggc
     1681 ggggttggcc atggtgtgcg gctcacggct caggcgccgg gcttctcctc ggcggtctcc
     1741 atcagcttgt catagacctg cgcgcgcggg gtgccggcgt cgatcagctt ctgcgcggcg
     1801 gccttgccct cgtcgacgag ccgggcgaac tcgctaaacg gcatcgcgcc gtttaggaac
     1861 cgcccgttga tgaagaacgc cggggtgccg ttgacgccga ccttgccggc gagcgcctcg
     1921 tcgtccttga tcatctgctc gatgtcggcg gcctccatgt cgagctcgaa catgccggtg
     1981 tcgaggccga gctcctgcgc gtagccgatc aggtcggcct tgccgagcgc cttttgattg
     2041 ctgaacagcc ggtcgtgcat ctcccagaac ttgccctggc ggtgggcggc gagcgccgcc
     2101 ttggcggccg ggcgcgcgtt ggcgtggaac gacagcggac gctggcggaa caccacgcgc
     2161 acgtcgttgc cgtaggtcga cttgacctcg tccatcgacg cgttgacgcg gctgcaaaac
     2221 gggcactcga agtccgagaa ctcgacgatc gtcaccagcg cgtcgtccgg gccggtctgc
     2281 gggcgggcgt cgaccgggac cttgtaggtc ttggccgggt cgggctcgcc ggggcgacgg
     2341 gcccgctgcg gctgcgcgcc cgcgttgccg ccgcgggtcg ccttggcgtc cttcatcagc
     2401 tcggcgtaga cctgatcgcg cggggtgccg gcgtcgacga gcgccttcgc ggccttgagc
     2461 tcgtactcga agacctgctt ccactggtgg gccggcaggg cgccgctgta gttgtggccg
     2521 ttgaccagga agtacggcgt gccgctgagg ccggccttgt tgccggacgc ctggtcgttg
     2581 tcgacggccg cgctggcggc cgcgctcttg cggtcgaggt cgaacttggc gacatcgagc
     2641 ccgatctgcc tggcgtactc ggtgaagtca gcgtcgccga gcttcttctg gttggcgaac
     2701 agctggtcgt gcatctccca gaacttgccc tgctggtgcg cggcccaggc ggcgcgcgcg
     2761 gccggggagg cctgcgcgtg cccgccgagc gggaagtgct tgaagatcag gcggacatcg
     2821 ccctcgtagc tgtccatcgc cgccttgagc ggctcggaca cgcgcgagca gtacgggcac
     2881 tggaagtccg accactcgac gatcgtgatc aaggcgtcgt tcgggcccat ctgcg
//
LOCUS       DASWNJ010000433         4631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_290968, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000433 DASWNJ010000000
VERSION     DASWNJ010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290968"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VGB85_07390"
     CDS             complement(<1..667)
                     /locus_tag="VGB85_07390"
                     /inference="COORDINATES: protein motif:HMM:NF013885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HEY0133885.1"
                     /translation="MLTTLQTILLGALLVILMAGMGASLTVADFREVLRRPRGPLIGL
                     LSQYGWMPLIAFVLCKALGLPDDLAISLIVVGCTPGGTTSNLFTYFAGADVALSVSMT
                     ALSTIAAVVLMPLSLYIYASGYASEGLKIPYGNITSTLAVMLVPLAIGMLVRARAPGA
                     ARMMERLGGLAGIAVLVLLIGTGIYNNLGLLGATTGTMYAAAGGLGLLGFGLGWLGAY
                     VLGL"
     gene            complement(681..2771)
                     /locus_tag="VGB85_07395"
     CDS             complement(681..2771)
                     /locus_tag="VGB85_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133886.1"
                     /translation="MSLACLLVSACRPARTTEPEPNLEPAPAPAMRARHTDGSPMVAV
                     ATSPVQLLPDTTVAVGSVAGIGALLAVVDVEAIIGKYRSHYDQMSALISGSTGFNLLD
                     PAQWREVGIDPDGPMGAAMLDMRSETVAGFITLSDPAKFRAFIDKLGGEKRLRPVIED
                     RGLVLKREPDSNTALVLRDGFLFVVTTDRPNEAPYDFARLLATIDPARGLTASPRYQR
                     AIASPEPPRPLTAYVDLWAIVQGQEVAREARARDTEPSWAEQELERAVQAGASAQEQA
                     RLRQQADEQRAWDKRHEQRRQREFELLRRWLAPVEPVVFELTASRGGVEGKIRAKMPE
                     TAPLRAVLQNAAMPSPVLSALGERAVLQLGGSVDVVAAIASFEDIVRASGDEPQKAYD
                     ELRRTLLVDLKTEVAPLLTGTGGFALTVSEALLRGEAKGEGKELGFALAVAVRDVPAA
                     QALLARLVQRAPIKAGKDAKTGAYMLSVPGYRTVHATVTAGQIVMTTDPGVIRRLGAG
                     SPASHGVPAAVVPVVTARDVGFVGMFDVVLPMFLLGARTSSVYMNTGDLFQPSVFPGV
                     DAQTIEKVPRSRAYKTKLRAWEALNTKILKEEQAQERRRMQLMLALAECFGVMAGNLR
                     EQPDGLELAGGQFFGKGGLTRALDLGVEQLTVTRGSERTWELYGQKSALEEELRRIRT
                     ADVATALRLPMPLE"
     gene            2965..4539
                     /locus_tag="VGB85_07400"
     CDS             2965..4539
                     /locus_tag="VGB85_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133887.1"
                     /translation="MSSRSTLLLLALPLACAAPAPADDSDSSSASGTTTDAPTTAATS
                     TGDGPETTGEPPPDTALLVHSFGAYDLAALEETEPCAQWTFNNEQPLYVNTVTLSNDG
                     GYHHSNWLVVPETSFVGEDGFFDCYDRGFHELLAAVAGTVLFAQSTQSRYETQELPAG
                     VVVKVPPRHKLIAGVHLLNLSTAPTRSELRMGLEIVHPRDVQTIVSPFRLSYEDLAIP
                     ALSESRFTGECLLDTQYAAKSGAAFDIKLYHVLPHYHYLGNYFSVEILGGPRDGEQIY
                     LHTGFNADGNGRTYDPPIDLTGARGLRFTCGYDNWRDKPIGYGIGDQEMCDMLALADM
                     RVMMNGAVDSGNKLVGADGDVLLHTAPCNAIALPKNASQGMPSAAERDAPLYIPPTAD
                     GDVDLDPVRPCIDTPADAAPAGPATLTNLRETVFGPACSFSSCHGGKTPAAVRLDGVD
                     LHALLLGPSAAPGIRLVTPGNPDDSWLYRLLSQCEPRDADGNLHTHMPYNAPNLLDPR
                     LVATVRAWIEAGAPPI"
BASE COUNT          703 a         1817 c         1455 g          656 t
ORIGIN      
        1 cgagcccgag cacgtaggcg ccgagccagc cgagcccgaa gccgagcagc ccgagcccgc
       61 cggccgcggc gtacatcgtc ccggtggtcg cgccgagcag cccgaggttg ttgtagatgc
      121 cggtgccgat cagcagcacg agcacggcga tgcccgcgag cccgccgagg cgctccatca
      181 tccgcgcggc ccccggcgcc cgcgcgcgca cgagcatccc gatcgccagc ggcacgagca
      241 tcaccgccag cgtcgaggtg atgttgccgt acgggatctt gagcccctcg gacgcgtagc
      301 cgctggcgta gatgtacagc gacagcggca tcagcacgac cgccgcgatc gtcgacagcg
      361 ccgtcatcga cacgctgagc gccacgtcgg cgccggcgaa gtacgtgaac aaattggagg
      421 tcgtgccgcc cggcgtgcag ccgaccacga tcagcgagat cgccaggtcg tcgggcaggc
      481 cgagcgcctt gcacagcacg aacgcgatca gcggcatcca cccgtactgc gacagcaaac
      541 caatgagcgg gccgcgcggg cgccggagca cctcgcgaaa gtccgcgacc gtcaggctcg
      601 cgcccatgcc agccatcagg atgaccagca gggctcccag caggatcgtc tggagcgtcg
      661 tcagcattgc gagcgtgact ctactccagc ggcatcggca ggcgaagcgc cgtcgcgacg
      721 tcggcggtac ggatccttcg cagctcctcc tcgagggccg acttttgccc gtacagctcc
      781 caggtccgct ccgagccgcg tgtgaccgtc agctgctcga ccccgaggtc gagcgcccgc
      841 gtcagcccgc ccttgccgaa gaattgcccg ccggccagct cgagcccgtc cggctgttcg
      901 cgcaggttgc cggccatgac cccgaagcac tccgcgagcg ccagcatcag ctgcatccgg
      961 cgccgctcct gggcctgctc ctccttgagg atcttcgtgt tgagggcctc ccacgcgcgc
     1021 agcttggtct tgtaggcgcg cgagcgcggg accttctcga tcgtctgcgc gtcgaccccg
     1081 gggaacaccg acggctgaaa cagatcgccg gtgttcatgt acacgctgct ggtgcgcgcg
     1141 ccgagcaaaa acatcggcag cacgacgtcg aacatgccga caaagccgac gtcgcgggcc
     1201 gtcaccaccg gcaccaccgc cgcgggcaca ccgtggctcg ccgggctgcc cgcgccgaga
     1261 cgacggatca cgcccgggtc ggtcgtcatg acgatctggc ccgccgtcac cgtcgcgtgg
     1321 accgtgcgat agcccggcac cgacagcatg tacgcccccg tcttcgcgtc cttgccggct
     1381 ttgatcggcg cccgctgtac gagcctggcc agcagcgcct gcgcggccgg cacgtccctg
     1441 accgcgaccg ccagcgcgaa gccgagctcc ttgccctccc ccttcgcctc gccgcgcagc
     1501 agcgcctccg agaccgtcag cgcgaagccc ccggtcccgg tcagcagcgg cgccacctcg
     1561 gtcttgaggt cgaccagcag ggtcctgcgc agctcgtcgt acgccttttg cggctcgtcg
     1621 ccgctggcgc gcacgatatc ctcgaagctt gcgatcgccg cgacgacatc gacgctgccg
     1681 ccgagctgca acaccgctcg ctcgcccagc gccgacagca ccggcgaggg catcgccgcg
     1741 ttttgcagca cggcccgcag cggcgccgtc tccggcatct tcgcgcggat cttgccctcg
     1801 acgccgccgc ggctcgcggt gagctcgaac accaccggct cgaccggcgc cagccagcgc
     1861 cggagcaact cgaattcccg ctggcgccgc tgctcgtggc gcttgtccca cgcgcgctgc
     1921 tcgtcggcct gctggcgcag ccgggcctgc tcctgcgccg acgcgccggc ctgcaccgcc
     1981 cgctcgagct cctgttcggc ccagctcggc tccgtgtccc gcgcccgggc ctcgcgcgcg
     2041 acctcctgcc cctgcacgat cgcccagaga tcgacgtagg cggtcagcgg ccgcggcggc
     2101 tccgggctgg cgatcgcccg ctgataccgc ggcgaggccg tcagcccgcg cgccgggtcg
     2161 atcgtcgcca gcaggcgggc gaagtcgtag ggcgcctcgt tcggccggtc ggtcgtgacg
     2221 acgaacaaaa agccgtcgcg cagcaccagc gcggtgttgc tgtcgggctc gcgcttgagc
     2281 accagcccgc gatcctcgat caccggccgc aggcgcttct cgccgccgag cttgtcgatg
     2341 aacgcacgaa acttggccgg gtccgacagc gtgatgaagc cggcgaccgt ctcgctgcgc
     2401 atgtcgagca tcgcggcgcc catcggtccg tcggggtcga tgccgacctc gcgccactgc
     2461 gccgggtcga gcaggttgaa cccggtgctc ccgctgatca gcgccgacat ctgatcgtag
     2521 tgactgcgat acttgccgat gatcgcctcg acatcgacga ccgccagcaa cgcaccgatc
     2581 cccgccacgc tgccgaccgc caccgtcgtg tccggcaaca gctgcaccgg cgaggtcgcc
     2641 accgccacca tcgggctccc gtccgtgtgc cgcgcccgca tcgccggcgc tggcgccggc
     2701 tccaggttcg gctccggctc cgtcgttcgt gcgggtcgac acgccgacac cagcaggcac
     2761 gccagactca cgccgcgaac cgttccagta cagagcccca tgcgacctcc gaggatagcc
     2821 cagcgtcgcc ggctcctggc ccgacaatgc aacatttcgc ggtctcggag caaagtccag
     2881 gcgcgcgcag gcgctcgcgg cggcgatgtg cccacccccg cccccggtgc tacaacgcag
     2941 ccttcgcccg cgagatcggt ggatatgtcg tcacgtagca ccctcctgct cctcgccctg
     3001 ccgctcgcct gcgccgcccc ggcccccgcg gacgactcgg actcctccag tgcgtccggc
     3061 acgaccacgg atgcgcccac gaccgccgcc acgagcacgg gcgacggccc ggagaccacc
     3121 ggcgagccgc cgccggacac cgccctgctc gtgcacagct tcggcgccta cgacctcgcc
     3181 gcgctcgagg agaccgagcc gtgcgcgcag tggacattta ataacgagca gccgctctat
     3241 gtaaacacgg tgacgctctc gaacgacggt ggttatcacc attcgaactg gctggtcgtg
     3301 cccgagacct cgttcgtcgg cgaggacggc tttttcgact gttacgaccg cggctttcac
     3361 gaattgctgg ccgcggtcgc cggcactgtc ctgttcgccc agtcgacgca gtcgcgctac
     3421 gagacccagg agctgccggc cggggtggtc gtcaaggtcc cgccgcggca caagctgatc
     3481 gccggcgtgc acctgctcaa cctcagcacc gcgccgaccc ggagcgagct gcgcatgggc
     3541 ctggagatcg tgcatccgcg cgatgtccag acgatcgtct cgccgtttcg cctcagctac
     3601 gaggacctcg cgatcccggc gctctcggag agccgcttca ccggcgagtg cctgctcgac
     3661 acgcaatacg ccgccaagtc cggcgccgcg ttcgacatca agctttatca cgtgttgccg
     3721 cactatcatt acctcggcaa ttacttctcg gtcgagatcc tcggcggccc ccgcgacggc
     3781 gagcagatct atctccacac cggcttcaac gccgacggca acggccgcac ctacgacccg
     3841 ccgatcgacc tcaccggcgc ccgcggcctg cgcttcacct gcggctacga caactggcgc
     3901 gacaagccga tcggctacgg catcggcgac caggagatgt gcgacatgct cgcgctcgcc
     3961 gacatgcgcg tcatgatgaa cggcgccgtc gactcgggga acaagctcgt cggggccgac
     4021 ggcgatgtcc tgctccacac cgccccctgc aacgcgatcg cgctgcccaa gaacgccagc
     4081 cagggcatgc cgagcgccgc cgagcgggac gccccactct acatcccccc gaccgccgac
     4141 ggcgacgtcg acctcgaccc cgtccggccg tgcatcgaca cgcccgccga cgccgccccc
     4201 gccggccccg ccacgctcac caacctgcgc gagaccgtgt tcggcccggc ctgcagcttc
     4261 tccagctgcc acggcggcaa gacccccgcc gccgtccgcc tcgacggcgt cgacctgcac
     4321 gccctgctgc tcggcccgtc cgccgcgccc ggcatccggc tcgtcacccc cgggaacccc
     4381 gacgacagct ggctgtatcg cctgctctcg cagtgcgagc cgcgcgacgc cgacggcaac
     4441 ttgcacacgc acatgcccta caacgccccg aacctgctcg acccgcgcct ggtcgcgacc
     4501 gtgcgcgcgt ggatcgaagc cggggcacca ccgatttagg tgcgatttac gtgcgcatgc
     4561 ggatcggcat gttcacggtg aagcgtgaac cgacgccggg gctgctctgc accgcgatgc
     4621 ggccgccgag c
//
LOCUS       DASWNJ010000434         4897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_8200, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000434 DASWNJ010000000
VERSION     DASWNJ010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8200"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1975)
                     /locus_tag="VGB85_07405"
     CDS             complement(<1..1975)
                     /locus_tag="VGB85_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0133888.1"
                     /translation="MTDEARMQAASREQERLWAALALHGASPVFHRCSSVWLHGEVDV
                     VRLADAVTRAAVRHELLRTTLVEVEGRLFQRIERSAPEGLVVESMEDPDAAVRAARAA
                     ACAVLDPRDAPPWRVRLIRTRAGRHLLLVVMHAVIADEQRSAAALLTELLTAGPEIFE
                     QRPANCPCEGPRDADVAWWRTQIDGAPPMLELASDRVRPPVRSFAGHLCRRSQGQDLA
                     RDLHALAAALATEPSTVALAVAAAWLYRHTGQQEFAIGVTLPSDEDRPGPAGEPVLLC
                     LRAGADDSLRALVERARQALEAAAEHGALAYMDAVEAARGVSPPFQVRLKKCGVAVAR
                     APGISPVDVSPPHIGYDLELGVDTSEGLALSIGCSADLFEADTAARFLDHWEVLLAAA
                     VAAPDRPISALATLTADERTTILSRWNPPATELPAPVRVHAQFEAQADRTPDAIAVTF
                     KNDESLSYRALDLRASAVARRLVELGVGPDVPVALYVERSLDLAVGVLAILKAGGAYV
                     PLDPSLPPTRMAWILEDTAVPVLVTQGALLGTLPAHSATHLLLDDFRGDAPDGQERVS
                     VATSPQDLAYVIYTSGSTGRPKGIGLPHVALFNLIEWHARTLLGGVATLQFASIGFDA
                     SFHEMFAAWRTGGVVHLIDETIRRDVVRLAEFIR"
     gene            complement(1972..2844)
                     /locus_tag="VGB85_07410"
     CDS             complement(1972..2844)
                     /locus_tag="VGB85_07410"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY0133889.1"
                     /translation="MALTSQQIEEFQQRGFLLLEDRFSPAEMQLLTREVGVIVGQRSE
                     RTVMEASGSAVRSVYAPHVSDPVFSVLARDPRLVEPARSLLGGDIYVYQSKLNIKSPF
                     VGEVWEWHQDYVFWHNEDKMPAARVLSAAVFLDDVNEFNGPMVLIPGSHRQGVLACPD
                     STNAGDAGQSGPAWRSHVSARLKYTIDRAVLQDLAGKHGLYAPKARAGSILLFDSNIA
                     HASAANISPFARSLIFYTYNHVDNAPPQAMLTRPDFLVSRDSRALAVASDDDLMNAAT
                     TPAANDGVTSLEAK"
     gene            3768..>4897
                     /locus_tag="VGB85_07415"
     CDS             3768..>4897
                     /locus_tag="VGB85_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY0133890.1"
                     /translation="MPTTNDRDERADGLTRRDLIAGLAGASLTAIAACDSAPRTPDAA
                     TAPTARKPRDEAPMQDDAVLSVKPLGGMPWETLDPFLFCVHHDDAYPAGNEQMGPATS
                     LAGRDIGQDFAGKDGWRMYHGDTIPGFPQHPHRGFETVTIVRRGLIDHSDSLGATARF
                     GGGDVQWLTAGAGIVHSEMFPLVDRENPNPLELFQIWLNLPRASKFAAPHFSMLWRES
                     IPTHVERDEQGRATTVTVIAGDLGDAKAPAPPPKSWAAQADSDVAIWTVDMAAGATWT
                     LPPAAAGTHRALYFFRGAGLTVGGRAVAARTLVRLRPDRAVTLVNGGEASELLVLQGR
                     PIGEPVVQHGPFVMNTRAEIQQAFADYQHTRFGGWPWPSDGPA"
BASE COUNT          768 a         1717 c         1725 g          687 t
ORIGIN      
        1 cgcggatgaa ttcggcgagt cgcacgacgt cgcggcgaat ggtctcgtcg atcaggtgga
       61 ccacgccgcc ggtccgccag gcggcgaaca tctcgtggaa gctggcgtcg aagccgatcg
      121 acgcgaattg cagcgtcgcc acgccgccga gcagcgtgcg cgcgtgccac tcgatcaggt
      181 tgaacagcgc gacgtgcggg aggccgatgc ccttgggccg cccggtcgaa ccggaggtgt
      241 agatgacgta ggcgaggtcc tgcggcgagg tcgcgaccga cacgcgctcc tgcccgtccg
      301 gagcgtcgcc gcgaaagtcg tcgagcagca agtgcgtggc cgagtgcgcc ggcagggtcc
      361 cgagcagcgc gccctgggtg acgagcaccg gcaccgcggt atcctcgagg atccacgcca
      421 tgcgcgtcgg cgggagcgag gggtcgagcg gcacgtaggc gccgccggcc ttgaggatcg
      481 caagcacgcc gaccgcgagg tcgagcgagc gctcgacgta cagcgcgacg gggacatccg
      541 gcccgacgcc gagctcgacc agccgtcggg cgacggcgct ggcccgcagg tcgagcgcgc
      601 ggtagctcag gctttcatca tttttaaagg tgacggcgat ggcgtcgggg gtgcgatcgg
      661 cctgcgcctc gaattgcgcg tgcacccgca cgggcgcggg gagctcggtg gcaggaggat
      721 tccagcgcga gaggatcgtg gtgcgctcgt cggcggtcag cgtcgccagc gcggagatcg
      781 gtcgatcggg cgcggcgacg gcggccgcga gcagcacctc ccagtgatcg aggaaacgcg
      841 cggccgtgtc cgcttcaaaa aggtcggcgc tgcagccgat gctcagcgcg aggccctcgc
      901 tcgtatcgac gccgagctcg aggtcgtagc cgatgtgggg cggcgagaca tcgacgggcg
      961 agatgcccgg cgcgcgtgcg accgcgacac cgcatttctt aagacggacc tgaaacggcg
     1021 ggctcacgcc gcgcgcggcc tcgacggcgt ccatgtacgc gagggcgccg tgctccgccg
     1081 ctgcttcgag cgcctgcctg gcgcgttcca ccagtgcgcg caggctgtcg tccgcgcccg
     1141 cgcgcaggca cagcagcacg ggctcccccg cgggtcctgg ccgatcctca tcggacggca
     1201 aggtgacccc gatggcgaat tcttgctgtc cggtgtggcg atacaaccac gcagccgcga
     1261 ccgcgagcgc cacggtcgag ggctcggtgg cgagcgcggc cgcgagggca tgaagatcgc
     1321 gagcgaggtc ttgcccctgc gagcgccggc acaggtggcc ggcgaacgac cgcaccggcg
     1381 gtcgcacgcg atcgctggcg agctcgagca tcggcggtgc gccgtcgatc tgcgtccgcc
     1441 accacgcgac gtccgcgtca cgcgggccct cgcaggggca gttcgccggg cgctgttcga
     1501 agatctccgg gccggccgtc agcagctccg tcagcagggc cgccgcgctg cgctgctcgt
     1561 cggcgatcac cgcgtgcatc acgaccagca acaggtgacg cccggcccgc gtgcgaatca
     1621 gacgcacccg ccacggcggg gcgtctcgcg ggtcgagcac cgcgcacgct gctgctctcg
     1681 ccgcgcgcac ggccgcgtcc ggatcctcca tcgactcgac gacgagcccc tcgggggccg
     1741 atcgctcgat ccgctggaac agccgaccct cgacctcgac gagggtggtc cgcagtaatt
     1801 cgtggcgcac cgccgcgcgg gtcaccgcgt cggcgagccg cacgacatcg acctcgccgt
     1861 gcagccacac gctgctgcag cggtgaaaca cgggtgaggc cccatgcagc gcgagcgcgg
     1921 cccacagccg ctcctgctcg cgcgaggccg cctgcatgcg cgcctcgtcg gtcatttcgc
     1981 ctccaaggac gtgacgccgt cgttcgcggc gggggtcgtc gccgcgttca tgaggtcgtc
     2041 gtcggacgcg acggcgaggg cgcggctgtc acggctgacg aggaagtccg ggcgcgtcag
     2101 catggcctga ggcggcgcgt tgtcgacgtg gttgtacgta taaaagatca gcgagcgcgc
     2161 gaacggcgag atgttggcgg cggaggcgtg ggcgatgttg ctgtcgaaca ggaggatcga
     2221 cccggcgcgg gccttgggcg cgtacaggcc gtgcttgccg gccaggtcct gcagcaccgc
     2281 gcggtcgatc gtgtacttga gccgggccga cacgtgcgat cgccacgcgg gcccgctctg
     2341 gccggcgtcc ccggcgttcg tcgaatccgg gcacgcgagc acgccctgac ggtgcgagcc
     2401 cgggatcagg accatcgggc cgttgaactc gttgacgtcg tcgaggaaca ccgccgcgga
     2461 cagcacgcgc gcggccggca tcttgtcctc gttgtgccaa aacacgtagt cctgatgcca
     2521 ctcccagacc tcgccgacga acggcgactt gatgttgagc ttgctctggt agacgtagat
     2581 atcgccgccc agcaggctgc gcgccggctc gacgagccgg gggtcgcggg ccagcaccga
     2641 gaacaccggg tcgctgacgt gcggggcgta gaccgaccgc accgcggagc ccgacgcctc
     2701 catgacggtc cgctcgctgc gctggccgac gatgacgccg acctcgcggg tcaacagctg
     2761 catctcggcg ggagagaagc ggtcctccag gaggaggaat ccacgctgct ggaattcctc
     2821 gatctgctgc gatgtaagtg ccattcgctg tactgctcct gagttcatgc gccaaaaggg
     2881 cgttcatgag cgaggggacc atcgaccaca atgcgcgcac cacgggcgca tcgcgcccgc
     2941 gcgccttcaa ccgtctacga tgacgaagat gatgatggag aaaatgaaga aacgtggccg
     3001 ccggccgcgc gggtgcaagt gcaagtgcaa gtgcaccttt taaaaggggg cggcgaagcc
     3061 agcgtctatg ctcgcaaggt acgcgacgct atcgcggttg taaagtcagc caagatattt
     3121 acttgcgatc gttgcaggtg cgtcaaggtg agcaggtcgc ccgtatctgg cttgccagcg
     3181 gtcgccgttg acgcggcgcc cgcgtataat tatctgtcct acatcgagcc cgcgatatct
     3241 ttgcctgtcg cacggagggc ctgtacgccg gacatatcgg ggtattcggg ggaacacccc
     3301 cgcggcgttg ggcccccaat ccacgcgcac aggaccgcgc aaagcccgtt tgggggcccg
     3361 tacgaggccc gtacgcaggc ccgggcgacg cgacgcaaac gggagtcgat ttcgacgaat
     3421 cggtgcaggt atggtgcaac gatcgaatct cgatgtttct gagccgatga tacttcgtgg
     3481 tcacgatccg tgagcacacg cgacgatgac ccgtgatccg ttcgtatcgt cgtgaccccg
     3541 gcgggccgcg atcgtcggac atcgccacga actatgtgga tatcagcgcc cgcgctcgac
     3601 ggcaccgccg atgcgcgctc gccccgggcg actcgcggcg cggcgcccct agcgcccggt
     3661 gcaatgctgg cgccgcgccc gcgcccgccg cgatcattgc accggcgaca acaatccgtt
     3721 cccgcgcccg cgcttacggc ccgcggcgcg cggcgcatac tgcccgggtg ccgacgacga
     3781 acgatcgaga cgagcgcgcc gacgggctca cgcgccgcga cctgatcgcg gggctggcag
     3841 gcgccagcct gacggcgatc gccgcgtgtg acagcgcccc acgaaccccc gacgccgcga
     3901 cggcgccgac cgcccgcaag ccacgagacg aggccccgat gcaagacgat gccgtgctca
     3961 gcgtgaagcc cctcggcggt atgccgtggg agaccctcga ccccttcctg ttctgcgtcc
     4021 accacgacga cgcctacccc gccggcaacg agcagatggg cccggcgacc tcgctcgccg
     4081 gccgcgacat cggccaggac ttcgccggca aggacggctg gcgcatgtac cacggggaca
     4141 cgatcccggg cttcccgcag cacccgcacc gcggcttcga gaccgtcacg atcgtgcggc
     4201 gcgggctgat cgaccacagc gactcgctcg gcgcgaccgc ccgcttcggc ggcggtgacg
     4261 tgcagtggct gaccgccggc gccggcatcg tccactcgga gatgttcccg ctggtcgacc
     4321 gcgagaaccc caacccgctc gagctgttcc agatctggct caacttgccg cgcgcgagca
     4381 agttcgccgc gccccacttc tcgatgctgt ggcgcgagtc gatcccgacg cacgtcgagc
     4441 gcgacgagca gggccgcgcg accacggtca ccgtgatcgc gggcgacctc ggcgacgcca
     4501 aggcccctgc cccgccgccg aagtcatggg ccgcccaggc cgacagcgac gtcgcgatct
     4561 ggaccgtcga catggccgcc ggcgcgacgt ggaccttgcc gcccgccgcc gccggcaccc
     4621 accgcgcgct ctacttcttt cgcggcgcgg ggctgacggt cggtggccgc gcggtcgctg
     4681 cgaggacgct ggtccgactg cgccccgatc gcgccgtcac cctcgtcaac ggcggcgagg
     4741 cgagcgagct gctggtgctg cagggccggc cgatcggcga gcctgtcgtc cagcacgggc
     4801 cgttcgtgat gaacacccgc gcggagatcc agcaggcctt cgccgactat cagcacaccc
     4861 gcttcggcgg ctggccgtgg ccgagcgacg ggccggc
//
LOCUS       DASWNJ010000435        12765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_608073, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000435 DASWNJ010000000
VERSION     DASWNJ010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 12765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12765
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608073"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VGB85_07420"
     CDS             complement(<1..750)
                     /locus_tag="VGB85_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133891.1"
                     /translation="MSTGTGTLRDVGTAVDFGTSQPIGCKGKVDLLFVISRLGTMKTE
                     QEQLVASFPGFVDTIEDKLAGFDVHIMTANPDGEWPGWNCETNQELCPKVKTDCGVYS
                     DGYQCGEYPDLFTVCDDRLGAGLTFNVGYGAANRLCELYGGNRYIISGEPNMNDALEC
                     IAKVGWLGPAPPMGEALVAALSPKLNGPDGCNAGFLRDDALLVVVLISDHADQESFTW
                     PHQWYDKIIEAKKDPNAVVMLGIVPHPPMAGE"
     gene            complement(940..2127)
                     /locus_tag="VGB85_07425"
     CDS             complement(940..2127)
                     /locus_tag="VGB85_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133892.1"
                     /translation="MDQATLFLLLGIGLCGAAAGCASDPEYPDYPLDGITVFVGFEEP
                     ICGGTFDWMEGRLRWLEAETGVAARVDPISYYWLREDVFDYCPTGACAKGDSIYTPLE
                     LFSHELVHGHLFQLGRPRPWLTEGMAVMLEDARWGAPSAPMTPSKMLAQSDALGLDYD
                     SAASFVRYLRDRFGMPAVIELYAALDHVDAQETPDVFLAVLGEDWRSVEEAYMAAYTP
                     VSVGSLNCDFPVLAPEEDTWKLPVASSWCEDAAAVGPFLGLDASFIPYSERYVIVEIP
                     EAGLYTLTMTSSAKASIGLIACDEPLDGFSWYDTEVNESIEFVPGRKRLTISTDIADK
                     AVGEVVIRREPSAMPGALGARESGTPGQIRGERSAAERPAPGGSWYPRHGQIRADSGR
                     GPV"
     gene            complement(2131..2511)
                     /locus_tag="VGB85_07430"
     CDS             complement(2131..2511)
                     /locus_tag="VGB85_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133893.1"
                     /translation="MVVPGCGDGCKQNGDGDTAGVLGRPCKAGTPADIAYLCPAGYFC
                     EFENNKDMLKPSELGKCAAMEQYKPCMNIRPCDSADFSPKCGSILDAAYCDWYQTSLR
                     CECVPPGPLVEGGDDADVKTPTTN"
     gene            2711..3907
                     /locus_tag="VGB85_07435"
     CDS             2711..3907
                     /locus_tag="VGB85_07435"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEY0133894.1"
                     /translation="MPACVRLSLLSVLLAACSGGGPTPSAPTPPPASSPTPVQAPTPQ
                     PVLAAATAPIPEAPQPTVTRAPLELTFVGDIIFGRYRADDTFDVIPGPDDHPFADIAE
                     LMRSDMLIGNLETPLTRDLPQKSPIGAQFRFGASKDMARHLVDGGFTALSLANNHAFD
                     LRAQGMLDSPVILQELGLIALGAARIEPPVFRVEAIEKASWKVGFLAITARRNAPHHE
                     GSPDLPFLSTNEFDDTLGPLIEAARAEHDLIVVFVHWGEEYADSPGAHQRRAAKALID
                     RGADLVVGHHPHVLQGIERHGRGAIVYSLGNFLFENTHEVPRLTGVLRARFDARRCLE
                     ALTFHPAYIKRTPSKHPVPATGGLGKQVRARMTTLSDEFSTDFETRGEDLVLADFPCD
                     PAAPGA"
     gene            complement(3932..5002)
                     /locus_tag="VGB85_07440"
     CDS             complement(3932..5002)
                     /locus_tag="VGB85_07440"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HEY0133895.1"
                     /translation="MNAPTTDLRTTPSERWREVWTARSQVATRPDADGLYLALAPMDG
                     VTDAVYRTLLTDLEGGRSGISVCVSEFVRVTRDAVPASVLRRDVPELDRGGRTPAGVP
                     VYVQLLGGVPEPVAQTARTAAEMGALGVDLNFGCPAKTVNNHDGGATLLKYPRRIHDI
                     VGRTRDLVPAHVPVSMKMRVGWDSADGIEEVARAAEAGGAAWITVHARTRVQLYQPPV
                     VWHALARARAVVKVPVVANGDLRSAADIQACAGQSQCEAFMIGRGAMARPELFARLRG
                     DEADMQRRMSRPAFAELLVRYHELLLASGVTASRALSRVKQWLRMAGDMDGALEPLFA
                     AIKVLPAWDPARAELLAWGAAA"
     gene            5099..5542
                     /locus_tag="VGB85_07445"
     CDS             5099..5542
                     /locus_tag="VGB85_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter C-terminal domain-containing
                     protein"
                     /protein_id="HEY0133896.1"
                     /translation="MPRRLCFVLCLTAIAGCHRKLGAAGATCKASADCAAELQCVAAT
                     CVDPPVLSAARAAEQAERARQEAIAAEQNKQMAKLLAELEALKAEQAALEQQKQALDS
                     KLGLIVDQTEKQRLLAEKAALDAALAQRDARTPAKPVSKAPPAGP"
     gene            complement(5547..5867)
                     /locus_tag="VGB85_07450"
     CDS             complement(5547..5867)
                     /locus_tag="VGB85_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133897.1"
                     /translation="MLPPPMSEQFREAIVEEAGKAGDRGVAMGQIVDAMVGHGHAVEA
                     VEQAIWQMLGERSLTPSGFVCRIVRRRDAFGEPVQARSYELLLVPWSADFDRQLELDL
                     TREA"
     gene            complement(5967..7055)
                     /locus_tag="VGB85_07455"
     CDS             complement(5967..7055)
                     /locus_tag="VGB85_07455"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEY0133898.1"
                     /translation="MTKTRIQRLAINTGGGDCPGLNAVIRAVVLHAHAHGIEVLGIED
                     GLDGLLPNKPAAVRRLTPAEVWPLLPEGGTILGTTNRGDPFAYKDSAGVQQDLSGALL
                     ERVRELGIDLLISVGGDGSQTIALRLQQLGLPVIGVPKTIDNDLDATVETFGFGTAVQ
                     VATDALDRLRTTAESHDRIMVLEVMGRDAGWIALHTGMAGGADVILLPELPYDPALVA
                     RDLRNTLASGRRFALIVVAEGAQSRAEHDGSPRERGARIRGGAGQRAADAIAAALPEV
                     ELRVTVLGHVQRGGTPTAHDRLLASRLGVEAVEAALRGESGVLVSSQPPEIRTVPLEQ
                     AVQKVRRVEHDAQILGHARALGISFGDR"
     gene            complement(7067..8113)
                     /locus_tag="VGB85_07460"
     CDS             complement(7067..8113)
                     /locus_tag="VGB85_07460"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY0133899.1"
                     /translation="MRICILGATGLVGRELLHLLPRAWPGSQLDLYASRDQELEHEGR
                     HFSVRGADALDAVDAQRGDLAFVALDDEHSARYVPRLRSLGYKVVDKSNTYRLDPQVS
                     LVVAGVNDGLVDSGADLVANPNCTTIPLALVLAPLRRRFGLRGVTVASYQAISGAGLA
                     TLDQFLLDARIGYTTPDRLGHQLPPGGYVGNTVPHAAKTDETGFSAEERKLMVESCKI
                     LAAPDLLISAQCCRVPVAVGHYEQVWIECDRPVDLAEAMAALADDREAPYVRVFPAPD
                     GAGLSALACVQDRDRALAGRLRRDPRVPEGRGLCLTVAGDNLRLGAATNAVRIATRWF
                     PAVDPTLQAPGMMP"
     gene            complement(8174..9235)
                     /gene="pyrC"
                     /locus_tag="VGB85_07465"
     CDS             complement(8174..9235)
                     /gene="pyrC"
                     /locus_tag="VGB85_07465"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY0133900.1"
                     /translation="MQYAPARTMTTLEIPRPDDWHLHLRDGEALRAVLPHTARQFARA
                     IVMPNLRPPVTTTAQAMAYRGRILEALPPEQVGRFEPLMTLYLTDTTAPEEVVRARES
                     GVVHGVKLYPAGATTNSAAGVTGLEGLAPVLEAMQACDMPLLVHGEVTDPATDVFDRE
                     AEFLRRQLAPLLERFPGLRVVVEHITTREAVAFVRAAGDRVAATITAHHLLLSRNAMF
                     TGGIRPHAYCLPVLKREVHRVALLEAATSGEAKFFLGTDSAPHARAAKEAACGCAGVY
                     TAHAAIELYAEAFASVGALAKLEGFASWFGPYFYGLPVNKERVRLTDEPWQVPASYAL
                     GDDVLVPMRAGEAVGWRLE"
     gene            9415..11343
                     /locus_tag="VGB85_07470"
     CDS             9415..11343
                     /locus_tag="VGB85_07470"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HEY0133901.1"
                     /translation="MDCTRMDDPDDTIPAAPARAMPTSMMSSGGEDMMATHRTLELRT
                     LGGELPPDLFGHIFIAGSIANPGRPAFSGEGTIYRVDLEGGAPTLKQSVFRPPCYLLD
                     QAIQHSGRGGLLKFHDLGLTRLSPLLGVRTVLSNSPIALHDRMIITTDAGRPWEFDPV
                     TLELVTPIGQMDEWVGAIPAPWLFPLVLTSAHPAEDEHSGELFTVNYANAGGGNPGFT
                     HLVRWSRGGPLEHFQLIDDNGEPIVIEQCVHQVALTRNHVILQDSAFVVEMRQMAFDA
                     AELLLPGLPIRDLLGGSQMRAQRPTTVLYLIPRHALQPRSGGVATAPATVQARRVEIP
                     GESVHFFVQYDDDEHLELIVPHTPTLDVSEWVHKGEVMLDGSRATEEIAGMQIPCALT
                     RGYLAVHTIDPYNGELLASRMTRSDETWGLALGTHAGNRQRRPIEAVFFNTSGFAPEL
                     VPQRVLKTYHDRVDAAMMPIATGKEPRLLDFDVPTGEILSYLCPHGWSLLSPTFVPRR
                     DSTGGPRDGYLVCLAHAADTVPRQPGTSGEEIWIFDAADISIGPICRLGHPELDFAFT
                     VHSSWVPQLRPSPRDYHVAMADDLDVDHVTRRSFGQLAFWDIFSAAVSSAMQHDTIKK
                     LLREQVLPHFADKPNPGR"
     gene            11403..>12765
                     /locus_tag="VGB85_07475"
     CDS             11403..>12765
                     /locus_tag="VGB85_07475"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0133902.1"
                     /translation="MAPTPPRGPGPLLLALGLLALGLVVVRLRLEPPPALPSDAPADR
                     FSAGRALDRLATLVGDDVPHPVGSPAADRVRARLLAEFTALGIPAEVQQGFACSARGA
                     SCGDVGNVVARLPGTHAGPAVLVSAHHDSVAAGPGAGDDGAGVSAVLELARVLSQGPA
                     PKNPVVFLLVDGEEAGLLGAQAFIDDHPHLKKDIGVTVNLDAGGTRGVTSITRTSPGN
                     ARVIDAFTAAVPRPYGASVIGAVYGLTPYDTDFSVYSKAEIASIDLGFGEDKAHYHTP
                     LDRLANLDPPSVQHLGDTALALVRALANADLESDAPGERVFLDALGLTMLAWPAPWTP
                     IFAALAVALLALATWRMRHVPGASSLNAKLLAAGSLPLLLAAPALLAGGLAWLLALLT
                     QAEVPGHAQPWPARVAIWSAALLASGLTTKWLTTRLGTARLTLALWWSLALLTAALAI
                     AAPD"
BASE COUNT         1894 a         5035 c         3978 g         1858 t
ORIGIN      
        1 ttcgcctgcc atgggcgggt gagggacgat gccgagcatg acgacggcgt ttgggtcctt
       61 cttggcctcg atgatcttgt cataccactg gtgcggccag gtgaaagact cttgatcggc
      121 gtggtcgctg atgagcacga cgacgagcag cgcgtcgtcc cgcaagaacc ccgcgttgca
      181 gccgtctggc ccgttgagct tgggagacag ggcggcgacc agcgcctcgc ccatcggcgg
      241 ggccgggccc aaccatccga ccttggcgat gcactcgagc gcgtcgttca tgttcggctc
      301 cccgctgatg atgtagcggt taccgccgta cagctcgcac agcctgttcg ccgcgccgta
      361 ccccacgttg aaggtgagcc cggccccgag ccgatcgtcg cacaccgtga acagatccgg
      421 gtattcgccg cactgatagc cgtccgaata cacgccgcaa tccgtcttga ccttggggca
      481 cagctcctgg tttgtctcgc agttccagcc cggccactcg ccgtccgggt tcgcggtcat
      541 gatgtggaca tcgaagcccg cgagcttatc ctcgatcgtg tcgacgaacc cggggaagct
      601 cgccaccagc tgctcctgct cggtcttcat cgtcccgagc cgcgagatca cgaacagcag
      661 gtcgaccttg cccttacagc cgatcggctg actcgtgccg aagtccaccg cggtccccac
      721 gtcgcgcagc gtgcctgtgc ctgtgctcat gcccgcgtcg ctgccggccg agtcgtcgcc
      781 tggcccggtg gacgtcgtgt cgccgccggt cgagcctccg cccgtcgccg ccgacgcact
      841 cgtgaccccc ggcccgctcg agaacccgga cggagaaggg tccccgcagg cggcgagggc
      901 cagagcaacg aggaccgagc tgcgcatcgc cgcgcgagtt tagaccgggc cgcgcccgct
      961 gtcagctcga atctgcccat gccgcggata ccacgacccg ccgggcgctg gccgctccgc
     1021 agcgctccgc tcgccgcgga tctggcctgg cgtcccggac tcgcgggcac cgagcgcccc
     1081 cggcatcgcc gatggctcgc gccgaatcac gacctcaccg accgccttat cggcaatatc
     1141 tgtcgagatc gtgaggcgct tgcgcccggg cacgaactcg atcgactcgt tgacttccgt
     1201 gtcataccag gagaacccgt cgagcggctc gtcacacgcg atcaggccga tgctggcctt
     1261 cgctgagctc gtcatggtca atgtatagag gccagcctcg gggatctcca cgatcacata
     1321 acgctcactg tatgggatga acgacgcatc caaaccgagg aacgggccga ccgcggccgc
     1381 gtcctcgcac cacgacgatg cgaccgggag cttccatgta tcttcctccg gcgcgagcac
     1441 ggggaagtca cagttgagcg accccacgga gaccggggta tacgccgcca tgtacgcctc
     1501 ctcgacggac ctccagtcct cgccgagcac cgccaaaaac acatccgggg tctcctgcgc
     1561 gtccacgtgg tccagcgcgg cgtacagctc gatgacggcc ggcatcccga agcggtcacg
     1621 caggtagcgg acgaagctcg ccgcggagtc gtagtcgagc cccagggcat ccgactgcgc
     1681 cagcatcttc gacggcgtca tcggtgcaga gggcgcgccc caccgcgcat cctcgagcat
     1741 gacagccatc ccctcggtca gccaaggcct gggccgtccg agctggaaca ggtgcccgtg
     1801 gacgagctcg tgggagaaca gctcgagcgg cgtgtagatg ctgtcgccct tcgcacacgc
     1861 cccggtcgga cagtagtcaa agacgtcctc gcgcagccag tagtacgaga tcggatccac
     1921 gcgcgcggcg acacctgtct ctgcctcgag ccagcgcagg cgcccctcca tccagtcgaa
     1981 ggtcccgcca cagatcggct cctcgaagcc cacgaacacc gtgatgccgt ccagcggata
     2041 atcggggtac tccgggtcgg acgcacagcc agctgcggcg ccgcacagcc cgatcccgag
     2101 caacaaaaac agggtggctt ggtccatcac tcaattcgtc gtcggcgtct ttacgtcggc
     2161 gtcgtccccg ccctcgacca gcggacccgg cggaacgcac tcgcagcgca ggctcgtctg
     2221 gtaccagtca cagtacgccg cgtctaggat ggacccgcac ttcggagaga agtcggccga
     2281 atcgcacggc ctgatgttca tacacggctt gtactgctcc atggccgcgc acttcccgag
     2341 ctccgatggc tttagcatgt ccttgttatt ttcgaattcg cagaagtaac ccgcggggca
     2401 gaggtacgcg atgtccgcgg gcgtccctgc cttgcagggt cgaccgagca cccccgccgt
     2461 atcgccgtct ccgttctgct tgcagccgtc gccgcacccc gggacgacca tcccaaggat
     2521 ggctaccaaa agaccaagtc gaaaatcttg caccatttcc agatcctcca cccgagccta
     2581 catcattttt gcgtgccctc tcccgatcgc tctcggcccg gcggcctggt gaacaacgtg
     2641 tcgccgcgct cgagttggag cctgcacaaa gctggcgtcg cggcggtgat ttgtgcacac
     2701 tcccgccgcc gtgcccgcct gcgtgcgtct ctcgctgctc tccgtgttgc tcgccgcctg
     2761 ctccggcggc ggcccgaccc cgtccgcgcc gacgcccccg cctgccagca gccccacgcc
     2821 ggtgcaggcc ccgacgccgc agcccgtgct cgccgccgcg accgcaccga tccccgaggc
     2881 tccgcagccg accgtcacgc gcgcgccgct cgagctgacc ttcgtcggcg acatcatctt
     2941 cggtcgctac cgggccgacg acaccttcga cgtcatccct ggtcctgacg atcatccctt
     3001 cgccgacatc gccgagctga tgcgctccga catgctgatc ggcaacctcg agacgccgct
     3061 gacgcgcgac ctcccgcaaa agagcccgat cggcgcccag ttccgcttcg gcgcctccaa
     3121 ggacatggcg cgccacctcg tcgacggcgg cttcacggcg ctgagcctcg ccaacaacca
     3181 cgccttcgac ctgcgcgcgc agggcatgct cgactcgccc gtcatcctgc aggagctcgg
     3241 cctgatcgcc ctcggcgccg cgcgtatcga gccgccggtg ttccgcgtcg aggcgatcga
     3301 gaaggccagc tggaaggtcg gcttcctcgc catcaccgcg cgccgcaacg cgccgcacca
     3361 cgagggctcg cccgacctgc ccttcctctc gaccaacgaa ttcgacgaca ccctcggccc
     3421 gctgatcgag gccgcccgcg ccgagcacga cctcatcgtc gtgttcgtgc actggggcga
     3481 ggagtacgcc gacagccccg gcgcgcacca gcgccgcgcc gcgaaggccc tgatcgaccg
     3541 cggcgccgac ctcgtcgtcg gccaccaccc gcacgtgctg cagggcatcg aacgccacgg
     3601 ccgcggcgcg atcgtctact cgctcggcaa cttcctgttc gagaacaccc acgaggtccc
     3661 gcgcctcacc ggggtcctgc gcgcccgctt cgacgcccgc cgctgcctcg aggccctcac
     3721 cttccacccc gcatatatta aacgcacccc cagcaagcac cccgtgcccg ccaccggcgg
     3781 cctcggcaag caggtccgcg cgcgcatgac caccctcagc gacgagttct cgaccgactt
     3841 cgagacccgc ggcgaggacc tcgtgctcgc cgacttcccc tgcgatcccg cggccccggg
     3901 cgcatgaaca gtgactcagc ggctcagcgg ctcacgccgc ggcgccccac gccagcagct
     3961 cggcgcgcgc cggatcccac gcgggcaaca ccttgatcgc cgcgaacaag ggctcgagcg
     4021 cgccgtccat gtcgccggcc atgcgcaacc actgcttcac gcgggacagc gcccggctcg
     4081 ccgtgacgcc gctggcgagc agcagctcgt gatagcggac gagcagctcc gcgaacgccg
     4141 gacgactcat cctccgttgc atgtccgcct cgtcgccgcg cagccgcgcg aacagctcgg
     4201 gccgcgccat cgccccgcga ccgatcatga acgcctcgca ctggctctgc cccgcgcacg
     4261 cctggatgtc ggccgcgctg cgcagatctc cgttggcgac caccggcact ttgacgaccg
     4321 cccgcgcccg cgcgagcgcg tgccacacca ccggcggctg atacagctgc acccgcgtgc
     4381 gcgcgtgcac ggtgatccac gcggcgccgc cggcctcggc cgcgcgcgcg acctcctcga
     4441 tgccgtccgc cgagtcccag ccgacccgca tcttcatcga caccggcacg tgcgccggca
     4501 ccaggtcccg ggtccgcccg acgatgtcgt ggatcctccg cggatatttt aagagggtcg
     4561 ccccaccgtc gtggttgttg accgtcttcg ccgggcaccc gaagttgagg tcgaccccca
     4621 gcgcgcccat ctccgccgcg gtgcgtgccg tttgggccac cggctccggc acaccgccga
     4681 gcagctgcac atacaccggc acgcccgccg gggtgcgccc gccgcggtcg agctcgggca
     4741 cgtcgcgccg cagcaccgac gccggcaccg cgtctcgcgt gacccgcacg aactccgaca
     4801 cgcacaccga gatcccgctg cgcccgccct cgaggtcggt caacaaggtg cggtagaccg
     4861 cgtccgtcac cccgtccatc ggcgccagcg cgaggtacag cccgtccgcg tccggccgcg
     4921 tcgcgacctg cgagcgcgcc gtccagacct cgcgccagcg ctcgctcggg gtcgtgcgaa
     4981 ggtctgtggt cggggcgttc atcgcgggcc acttacttag ggccccggcg ccccacggtc
     5041 aacccgcgtc gccccgccgc ttgcgacgcc gcgggcaacc cgctctactg acgaagcaat
     5101 gccaaggcgc ctgtgcttcg tcctgtgcct gaccgcgatc gcaggctgtc accgcaagct
     5161 cggggccgcc ggtgcgacgt gcaaggcctc ggcggactgc gccgccgagc tgcaatgcgt
     5221 cgccgccacc tgcgtcgacc cgcccgtgct ctccgccgcg cgcgccgccg aacaggccga
     5281 gcgagcccgc caggaggcga tcgctgccga acagaacaag cagatggcga agctgctggc
     5341 cgagctcgag gccctcaagg ccgagcaggc cgcgctcgag cagcaaaagc aggcgctcga
     5401 cagcaagctc ggcctcatcg tcgaccagac cgagaagcag cgcctgctcg ccgagaaggc
     5461 cgcactcgac gcagcgctcg cgcagcgaga cgccaggacg cccgcgaagc cggtctcgaa
     5521 agctccgccc gcaggcccgt gacggctcac gcctcgcgcg tcaggtcgag ctcgagctgg
     5581 cggtcgaagt ccgccgacca cgggaccagc agcagctcgt agctgcgcgc ctgcaccggc
     5641 tcgccgaacg cgtcgcggcg gcgaacgatc cggcacacga agccgctcgg cgtcaggctg
     5701 cgctcgccga gcatctgcca gatcgcctgc tcgaccgcct cgaccgcgtg gccgtggccg
     5761 accatcgcgt cgacgatctg acccatcgcg accccgcggt cgcccgcctt gccggcctcc
     5821 tcgacgatcg cctcgcggaa ctgctcgctc atcggaggcg ggagcatgga ccgactccgg
     5881 gcccctggca accgcccaga acggtgatcc tcgcccattc acagccatct acctgtccca
     5941 aagaacaggc acgcgccgcg accttttcag cggtcgccga agctgatgcc gagcgcccgc
     6001 gcgtggccga ggatctgcgc gtcgtgctcg acgcgacgga ccttctgcac cgcctgctcg
     6061 agcggcaccg tgcggatctc cggcggctgc gaggacacca gcacgcccga ctcaccgcgc
     6121 agcgccgcct cgaccgcctc gacgccgagc cgcgacgcca gcaacctgtc gtgggccgtc
     6181 ggcgtaccgc cgcgttgcac gtggccgagc accgtcacac gcagctcgac ctccggcagc
     6241 gccgccgcga tcgcgtccgc ggccctttgc cccgcgccgc cgcggatcct cgcgccacgc
     6301 tcgcgcgggc tgccgtcgtg ttcggcccgc gactgcgcgc cctcggcgac gacgatcagc
     6361 gcgaagcggc ggccgctcgc cagcgtgttt cgcaggtcgc gggcgaccag cgcggggtcg
     6421 tagggcagct cgggcagcaa gatgacatcg gcgccgccgg ccatcccggt gtgcagcgcg
     6481 atccacccgg cgtcgcgtcc catcacctcg agcaccatga tgcggtcgtg cgactccgcg
     6541 gtcgtgcgca ggcggtcgag cgcgtcggtc gcgacttgca ccgcggtgcc gaagccaaag
     6601 gtctcgaccg tcgcgtcgag gtcgttatca attgtcttcg gcacgccgat caccggcagc
     6661 ccgagctgtt gcaggcgcag cgcgatcgtc tgtgagccgt cgccgccgac gctgatcagc
     6721 agatcgatcc cgagctcgcg gacccgctcg agcagcgcgc ccgacagatc ctgctgcacg
     6781 ccggccgagt ccttgtatgc aaaaggatcg ccacggttgg tcgtgccgag gatcgtgccg
     6841 ccctccggca acagcggcca cacctcggca ggcgtcagtc ggcgcaccgc cgccggcttg
     6901 ttcggcagca agccgtcgag gccgtcctcg atgccgagca cctcgatccc gtgcgcgtgc
     6961 gcgtgcagga ccaccgcgcg gatcaccgcg ttgagcccgg ggcagtcgcc gccgccggtg
     7021 ttgatcgcca gccgctggat ccgtgtcttc gtcatgagcc ccgctgtcat ggcatcatgc
     7081 ccggcgcctg caaggtcggg tcgaccgccg ggaaccaccg cgtggcgatc cgcaccgcgt
     7141 tggtggccgc tccgagccgc aagttgtccc ccgcgaccgt caggcacagc ccgcgtccct
     7201 ccggtacgcg cgggtcccgg cgcaggcggc cggcgagcgc ccggtcccgg tcctgcacgc
     7261 aggccagcgc gctcaggccg gcgccgtcgg gcgccgggaa cacgcgcacg tacggcgcct
     7321 cgcggtcgtc ggccagcgcc gccatcgcct cggcgaggtc gaccggccga tcgcactcga
     7381 tccacacctg ctcgtagtga ccgaccgcga ccggcacgcg gcagcactgc gccgagatca
     7441 gcaggtccgg cgccgcgagg atcttgcagc tctcgaccat cagcttgcgc tcctccgccg
     7501 agaagccggt ctcgtcggtc ttcgccgcgt gcggcacggt gttgccgacg tagcctcccg
     7561 gcggcagctg gtgcccgagg cggtccggcg tcgtgtagcc gatgcgcgcg tcgagcaaaa
     7621 attgatcgag cgtcgcaagc cccgcgccgg agatcgcctg atacgacgcg accgtcacgc
     7681 cgcgcagccc gaacctccgc cgcagcggcg cgagcaccag cgcgagcggg atcgtcgtac
     7741 aattcgggtt ggcgaccaga tcggcgccgg agtccacgag cccgtcgttg acccccgcga
     7801 ccaccagcga cacctgcggg tcgaggcggt acgtgttgct cttgtcgacc accttgtagc
     7861 cgagcgagcg cagcctcggc acatagcgcg cgctgtgctc gtcgtcgagg gcgacgaagg
     7921 ccaggtcacc gcgctgcgcg tcgacggcgt ccagcgcgtc ggcgccgcgc accgagaagt
     7981 gccgcccctc gtgctcgagc tcctggtctc gactggcata caggtcgagc tgcgaacccg
     8041 gccacgcgcg cggcaacagg tgcagcagct cacgaccgac gagaccggtg gctccaagga
     8101 tgcagatccg catgcggccg cagcgtaaca aaccccgccc cgcccggcga gcgcgtttgc
     8161 accgccaaac tcgctattcc agccgccagc cgaccgcctc gcccgcgcgc atcggcacca
     8221 gcacgtcgtc cccgagggcg tagctcgccg gcacctgcca cggctcgtcc gtcaacctca
     8281 cccgctcctt gttgaccggc agcccgtaga agtacggccc gaaccagctg gcgaagccct
     8341 cgagcttcgc cagcgcgccg accgacgcga acgcctccgc gtacagctcg atcgccgcgt
     8401 gggccgtgta caccccggcg cagccgcacg ccgcctcctt ggccgcgcgc gcgtgcgggg
     8461 cgctgtcggt gccgaggaag aacttcgcct caccgctcgt cgccgcctcg agcagcgcca
     8521 cgcgatgcac ctcgcgcttg agcaccggca agcagtacgc gtgcgggcgg atccccccgg
     8581 tgaacatcgc gttgcggctc agcagcaggt gatgcgccgt gatcgtcgcg gccaccctgt
     8641 cgcccgccgc gcgcacgaac gcgaccgcct cgcgcgtcgt gatgtgctcg accaccacgc
     8701 gcagcccggg aaatcgctcg agcaacggcg cgagctgcct ccgcaagaat tccgcctcgc
     8761 ggtcgaacac atccgtggct ggatcggtga cctcgccgtg gaccagcaac ggcatgtcgc
     8821 acgcctgcat ggcctcgagg acaggtgcca gcccctcgag ccccgtcacc ccggcggccg
     8881 agttggtggt cgcgcccgcc gggtacagct tgaccccgtg gaccaccccg ctctcgcgcg
     8941 cgcgcacgac ctcctcgggc gccgtcgtgt cggtcaggta cagcgtcatc agcggctcga
     9001 agcggccgac ctgctccggc ggcagcgcct cgaggatgcg cccgcgatac gccattgcct
     9061 gcgccgtcgt cgtcaccggc gggcgcaggt tcggcatcac gatcgcgcgc gcgaactgcc
     9121 tggcagtgtg gggcagcacg gcgcgcaacg cctcgccgtc gcgcagatgc aggtgccagt
     9181 cgtcgggccg cgggatctcg agcgtcgtca tcgtgcgcgc gggagcgtac tgcatcgccc
     9241 gcgctcgtcg gcgatctccc tcgccccgtc acgatcgtcg ggcgatgtgc ccggatctgc
     9301 gcattcgtgg gcacaatgac ggccacatcg gcctccggcc caggtgtgct tgagcttgcg
     9361 cacgaacagt cgggcatcca cggggatgcc tcgtgtcgct cactgtgccg gaccatggac
     9421 tgcacgcgga tggacgaccc cgacgacacg atccccgccg cccctgcgcg ggcgatgccc
     9481 acctcgatga tgagcagcgg gggcgaggac atgatggcga cccatcgcac gctcgagctg
     9541 cgcacgctcg gcggcgagct gccgccggac ctgttcggtc acatcttcat cgccggatcg
     9601 atcgccaacc cgggtcgtcc ggccttctcg ggcgagggca ccatctatcg cgtcgacctc
     9661 gagggcggcg ctccgaccct caagcagtcc gtgtttcgcc cgccctgcta cctgctcgac
     9721 caggcgatcc agcactccgg tcgcggcggc ctgctcaagt tccacgacct cgggctgacg
     9781 cgcttgtcgc cgctgctcgg cgtccgcacc gtgctcagca actcgccgat cgcgctgcac
     9841 gaccgcatga tcatcaccac cgacgccggc cgtccgtggg agttcgaccc ggtcacgctc
     9901 gagctcgtca ctcccatcgg ccagatggac gagtgggtcg gggcgatccc cgcgccgtgg
     9961 ctgttcccgc tggtcctcac ctccgcgcac ccggccgagg acgagcactc cggcgagctg
    10021 ttcaccgtca actacgccaa cgctggcggc ggcaaccccg gcttcaccca cctggtccgc
    10081 tggagccgcg gcggcccgct cgagcacttc cagctgatcg acgacaacgg cgagccgatc
    10141 gtcatcgagc agtgcgtgca tcaggtcgcg ctgacccgca accatgtcat tttgcaagac
    10201 tccgcgttcg tcgtcgagat gcgccagatg gccttcgacg ccgccgagct gctgctaccc
    10261 ggcctgccga tccgcgacct cctcggcggc agccagatgc gggcgcagcg gccgaccacc
    10321 gtgctttatt tgatcccccg ccacgcgctg caaccccgca gcggcggcgt ggcgacggcc
    10381 ccggcgaccg tccaggcccg gcgcgtcgag atccccggcg agtcggtcca cttcttcgtg
    10441 caatacgacg acgacgagca cctcgagctg atcgtcccac acaccccgac gctcgacgtc
    10501 tccgagtggg tccacaaggg cgaggtcatg ctcgatggtt cgcgcgccac cgaggagatc
    10561 gccggcatgc agatcccctg cgcgctgacc cgcggctacc tcgccgtcca caccatcgat
    10621 ccttacaacg gcgagctgct ggcgtctcgc atgacccgca gcgacgagac ctggggcctc
    10681 gcgctcggca cccacgccgg caatcgccag cgccgcccga tcgaggccgt gttcttcaac
    10741 accagcggct tcgcgcccga gctcgtgccg cagcgcgtgc tcaagaccta tcacgaccgc
    10801 gtcgacgcgg cgatgatgcc gatcgccacc ggcaaggagc cgcgcctgct cgacttcgac
    10861 gtcccgaccg gcgagatcct cagctatctg tgtccgcacg gctggagctt gctgtccccg
    10921 acctttgtcc cgcgccgtga cagcaccggc ggcccgcgcg acggctacct cgtctgcctc
    10981 gcccacgccg ccgacaccgt gccgcgccag cccggcacca gcggcgagga gatctggatc
    11041 ttcgacgccg ccgacatcag catcggaccg atctgtcgcc tcggtcaccc cgagctcgac
    11101 ttcgcgttca ccgtccacag cagctgggtc ccccagctgc gaccgagccc ccgcgactac
    11161 cacgtcgcga tggccgacga cctcgacgtc gaccacgtca cccgcagatc cttcggtcag
    11221 ctcgcgttct gggacatttt ttcagccgcc gtctcctccg cgatgcaaca cgacacaatt
    11281 aaaaagttgc tgcgcgagca ggtcctgccg cacttcgccg acaagccgaa cccgggtcgc
    11341 tgaccgacct ggcccgagag acatcttccc tcgcgcgtcg accgcgtgtt agcgtccccg
    11401 tgatggcccc cacgcccccg cgcggccccg gcccgctgct gctcgccctc ggcctgctcg
    11461 ccctcggcct cgtcgtcgta cgcctgcgcc tcgagccacc gcccgcgctg cccagcgacg
    11521 cccccgccga ccgcttctcg gccggccgag ccctcgaccg cctcgccacc ctcgtcggcg
    11581 acgatgtccc gcacccggta ggttcgcccg ccgccgaccg cgtgcgcgcg cggctgctcg
    11641 cagagttcac cgccctcggg atccccgccg aggtgcaaca gggcttcgcc tgctccgcgc
    11701 gtggtgccag ctgcggcgac gtcggcaacg tcgtcgcccg cctgccgggc acgcacgcgg
    11761 gcccggccgt gctcgtctcg gcgcaccacg actcggtggc agccgggccg ggcgccggtg
    11821 acgacggcgc cggcgtctcc gcggtgctcg agctggcgcg cgtcctgtct caagggccag
    11881 ccccgaaaaa cccggtcgtc ttcctcctgg tcgacggcga ggaggccggc ctgctcggcg
    11941 cgcaggcctt tatcgacgat cacccgcatt taaaaaaaga catcggcgtc accgtcaacc
    12001 tcgacgccgg gggtacgcgc ggcgtcacct ccatcacgcg caccagcccc ggcaacgcgc
    12061 gggtgatcga cgccttcacc gccgccgtgc caaggcccta cggcgcctcc gtcatcggcg
    12121 ccgtgtacgg cctgaccccc tacgacaccg acttcagcgt ctacagcaag gccgagatcg
    12181 ccagcatcga cctcggcttc ggcgaggaca aggcccacta ccacaccccc ctcgaccgcc
    12241 tcgccaacct cgacccgccg agcgtgcaac acctcggcga caccgccctc gcgctcgtcc
    12301 gcgcgctggc gaacgccgac ctcgagtccg atgcccccgg cgagcgcgtc ttcctcgacg
    12361 ccctcggcct caccatgctc gcctggcccg ccccgtggac ccccatcttt gcggcgctgg
    12421 cggtcgcgct gctcgccctc gcgacctggc gcatgcgaca tgtccccggg gccagctcac
    12481 tcaatgccaa actgctcgcc gccggaagcc tcccgctgct gctcgccgcc cctgccctgc
    12541 tcgcgggcgg cctcgcctgg ctgctcgctc tgctgacgca ggccgaggtc cccggccacg
    12601 cgcagccctg gcccgcccgc gtcgcgatct ggtccgccgc cctgctcgcc agcggcctca
    12661 ccacaaaatg gctgaccacc cgcctcggca cagcccgcct gaccctcgcc ctgtggtggt
    12721 ccctcgcgct gctgaccgcc gccctcgcga tcgccgcccc agatc
//
LOCUS       DASWNJ010000436         1535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_502360, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000436 DASWNJ010000000
VERSION     DASWNJ010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502360"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="VGB85_07480"
     CDS             <1..821
                     /locus_tag="VGB85_07480"
                     /inference="COORDINATES: protein motif:HMM:NF037435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HEY0133903.1"
                     /translation="AAKELPALRAATADLSWLLSHGYAEQSALKLVGDRHELTVRQRQ
                     AVSRCACSDAARDRRLARRITRDEFLARDPPTLAIDGFNCLITIESALSHGIVLRGRD
                     GALRDLASVHGTYRQVAETAFAVEMLGRHLHGPREAQPPAPRTDTHTPPDPPPLAVTW
                     FLDRPVANSGRLRALLLEIAERRGWPWTCELVNNPDAVLIRPEHPVVASSDAFILEHC
                     ARWFDPLGSLLAAGSPSASAAHVPTSGPVLAARPGLWLLDLGSPSPSSSGIGET"
     gene            821..>1535
                     /locus_tag="VGB85_07485"
     CDS             821..>1535
                     /locus_tag="VGB85_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133904.1"
                     /translation="MASWLRLSTVVRGAMITGLISGCGASNTADALIGPEGGVVAHDD
                     GVTLTVPPGALLVTRQIIIRPSDEKLSRGTFEQVGNSYLIEPRDLEFRVPAELTFDTD
                     VPERPSVLLKPGETRVIAYSSADDKPTAYIGSLGVVAAAKGGEPAGVVDSPKLARTPT
                     GSDLPPPAIDAAELKISPKGVTSLDVGFTAFDTSGMSKSLLNGEGTAYCGFKFGPAVL
                     GASISGGCSGGITSGTLALN"
BASE COUNT          204 a          645 c          467 g          219 t
ORIGIN      
        1 tcgccgcgaa ggagctcccg gccctgcgcg ccgccacggc cgacctctcg tggctgctgt
       61 cgcacggcta cgccgagcag tccgcgctca agctcgtcgg cgaccgccac gagctgaccg
      121 tgcgccagcg ccaggccgtc agccgctgcg cctgcagcga cgccgcccgc gaccgccgcc
      181 tcgcccgccg gatcacccgc gacgagttcc tcgcgcgcga ccccccgacg ctggcgatcg
      241 acggcttcaa ctgcctgatc acgatcgagt ccgcgctgtc ccacggcatc gtcctgcgcg
      301 gccgcgacgg cgccctgcgg gacctcgcca gcgtgcacgg cacctaccgc caggtcgcgg
      361 agaccgcctt cgccgtcgag atgctcggcc gccacctcca cggtccgcgc gaagcacaac
      421 cgcccgcgcc ccgcaccgac acccacacgc cccccgaccc gccgccgctc gccgtcacct
      481 ggttcctcga tcgccccgtc gccaacagcg gccgtttgcg cgccctcctg ctcgagatcg
      541 ccgagcgccg cgggtggccg tggacctgcg agctcgtcaa caaccccgac gccgtcctga
      601 tccgccccga gcaccccgtg gtcgccagca gcgacgcctt catcctcgag cattgcgcgc
      661 gctggttcga tccgctcggg agcttgctcg cggccggttc gccctccgcc agcgctgcgc
      721 acgtccccac ctcgggcccg gttctggccg ccaggccggg cctgtggttg ctcgacctcg
      781 gctccccgtc gccgtcgagt tcgggtatag gtgagacgtg atggcttcct ggcttcgtct
      841 ctcgaccgtc gttcgtggcg cgatgatcac tggcttgatc agtggttgcg gcgccagcaa
      901 caccgccgac gcgctgatcg gccccgaggg cggcgtcgtc gcccacgacg acggcgtcac
      961 cttgaccgtg cccccgggcg cgctgctggt cacccggcag atcatcatcc gccccagcga
     1021 cgagaagctc tcgcgcggca ccttcgagca ggtcggcaac agctacctca tcgagccgcg
     1081 tgacctcgag tttcgtgtcc ctgccgagct gaccttcgac accgatgtcc ccgagcgccc
     1141 gagcgtcctg ctcaagcccg gcgagacccg cgtcatcgcc tacagctccg ccgacgacaa
     1201 gccgaccgcg tacatcggca gcctcggcgt cgtcgcggcc gccaagggcg gcgaaccggc
     1261 cggcgtcgtc gactccccga agctcgcgcg caccccgacc ggctccgacc tgcccccgcc
     1321 cgcgatcgac gccgccgagc tcaagatctc gcccaagggc gtcaccagcc tcgacgtcgg
     1381 cttcaccgcc ttcgacacca gcggcatgtc caagagcctg ctcaacggcg agggcaccgc
     1441 ttactgcggc ttcaagttcg gcccggctgt cctcggggcc agcatcagcg gcggctgctc
     1501 gggcggcatc accagcggca ccctcgccct caacg
//
LOCUS       DASWNJ010000437         6406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_502372, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000437 DASWNJ010000000
VERSION     DASWNJ010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6406
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502372"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VGB85_07490"
     CDS             <1..991
                     /locus_tag="VGB85_07490"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0133905.1"
                     /translation="VIATGTAPHVPGPFAKLPPELLLSNESVFELADLPASVLIVGAG
                     PIGLELGQALHRLGVRVTIAGTRGAVGPLRDPRVKAAALAALCGELELHTDYVAGEIA
                     AGGDGVTMRYICDGEHRSGTWARVLLAAGRKSLLGELGLEHAGVVLDGKGRPQGFDAQ
                     TLRIGETNVFLAGDVSGLRPLLHEASDEGHIAGGNAARLPDGVQPQPRRTKLGVVFTD
                     PGMAVVGDGFEGVTPETHAVGEVDWSRQGRARVLRKNYGVSRIYATRSDRKLHGAELA
                     GPGAEHMAHLLAWAVQQGLTVDDALRMPFYHPVLEEGLRTALQALKADLGKVV"
     gene            1097..1417
                     /locus_tag="VGB85_07495"
     CDS             1097..1417
                     /locus_tag="VGB85_07495"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY0133906.1"
                     /translation="MKIRFFETNRGQAPVREYIDSLPTRDVAKIGAALSDIAEHGLRD
                     ALTQLRQIRGTLWEIKIQPHRIFYAVVEGDTLVLLHAYRKQSQRAPKNEIATALGRLK
                     VLTE"
     gene            1428..1859
                     /locus_tag="VGB85_07500"
     CDS             1428..1859
                     /locus_tag="VGB85_07500"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0133907.1"
                     /translation="MTGPRKKSSPGSKRAHVGADVMAYFRDLAAADPEFATASREEAD
                     KLKLARRIRELREAKGLSQTQLAEMIETKQPSIARIESGRALPRLEVLQRIAAALGMR
                     LTIEFEKVPAPRRKRAKAAVVEHTATWTNDESRPSARDHAR"
     gene            1849..2676
                     /locus_tag="VGB85_07505"
     CDS             1849..2676
                     /locus_tag="VGB85_07505"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY0133908.1"
                     /translation="MRDSWYLALVLASCHAAPAAAPVAETVKADAGSFTMHSRALGEV
                     RRINIYTPPGYADSGLRYPVLYMPDGGLEEDFPHVTEDIEAAVRAGQMRAMIVVGIEN
                     TERRRDMTGPTMNEEDKKIAPRVGGSAQFRAFIRDELMPEIGRRVRGDGETAIVGESL
                     AGLFVLETFFAQPELFDTYVALSPSLWWNDKALLRGAAARLHERPRWPGTLYFATAGD
                     DDPDGDGEALAAILRAAAPVGLTWSYQKHPDLLHSTIYRGASPAVFRRLFPPVAVRR"
     gene            complement(2687..3946)
                     /locus_tag="VGB85_07510"
     CDS             complement(2687..3946)
                     /locus_tag="VGB85_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0133909.1"
                     /translation="MSATIQNPDFLAAHNPYDPAQMVDPYPFYARARQATAAFYNAQL
                     SSWMVTRPDDIQAIYRDPARFSSKYIMSPHSALPPEVEAVFAGQDPWLPTLLTTDPPD
                     HTRMRSLLIKGFSPQAIAAREPAIRGLADELIDGFVADGETDIVQRFAFPFTAMVIGD
                     MLGVPRADITRLKTWSGDWIRLVWCADPLERLVESAHGFLAFQRYFQDMIAERRKHPG
                     DDVLSLLIQARLADGDRLSERELVAVPMQLIIGGHETTLFAISNALDALLREPADLAA
                     VRADPGLLPQFIEESIRHQTPLLVNQRTALTEVKLGDVTIPANSSLLLLIPAANRDPA
                     VFPDPDRLDMHRENADRHFSFGRGIHFCIGAALSRLEMRVGLERLLERLPGLRIAPDR
                     PRERAPGAMFFGYEHLHLAWDRAGSAA"
     gene            complement(4128..4493)
                     /locus_tag="VGB85_07515"
     CDS             complement(4128..4493)
                     /locus_tag="VGB85_07515"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEY0133910.1"
                     /translation="MKNTIIHTLLGFVALTCTTPASAAEGQLDKDQISGVVKAHISEI
                     RACYNQALSKDPQASGKIVIDFTIGDKGTVTKTAVAESSMKDAAVPTCMSAAIEGWLF
                     PKPDGGSVEVTYPFVLEPG"
     gene            complement(4634..5620)
                     /locus_tag="VGB85_07520"
     CDS             complement(4634..5620)
                     /locus_tag="VGB85_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133911.1"
                     /translation="MHSMDLQPPVRRDDARTVLAMVAALAIPLMITLQSVQSPAVLVM
                     TSDNPTPYGYTWSLALWVVPLTVLVVWLQRSPGFHLPRLAFWRTVAALAALGVVLDVA
                     LGDAFFKFPNHGATLGINLYGFEVASMSMVPNVPVEELAFYFFGVAFTLVLYLWCDLH
                     WFGLYGRSLRERLPPRGARIRFEPRALLLGLALIGVAFAYKRLCSPVPDGFPAYFAFL
                     VLVAFVPACMVFPVVRHRVNWRAFSFTALVMFTISTWWEAMIAFPYQWWTYRDEMMIG
                     VFIRAWNGLPIEEPCLWLLVSFTTVLVFEANLLHLLARRSPDELLFVRRDLP"
     gene            complement(5665..6324)
                     /locus_tag="VGB85_07525"
     CDS             complement(5665..6324)
                     /locus_tag="VGB85_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133912.1"
                     /translation="MERDRRRLFVVLRTMAYALLAQVVSAICSLGWLTTSVVTAALLG
                     VLVAHAWRERDVVLAKILLFGLVVGFGELPADHFGVVVTETLVYSPGGPYVGVTPLYM
                     PFGWVVMMVQLGVLAGWLAPRWGPLRACVALAVLGGLNIPAHELLAKYADLWHYQNTP
                     MVLGAVPHYVIVAEAMLGLVIPLLLLRVATTTWRGVVGLGALQALAIYVAGRIAFALV
                     G"
BASE COUNT         1130 a         1972 c         2363 g          941 t
ORIGIN      
        1 ggtcatcgcg accggcacgg cgccgcacgt gccggggccg ttcgcgaagt tgccgccgga
       61 gctgttgctc agcaatgagt cggtgttcga gctggccgac ctgccggcga gcgtgctcat
      121 cgtcggcgcc ggaccgatcg ggctcgagct cgggcaagcg ctgcaccggc tcggggtgcg
      181 cgtgacgatc gcaggcacgc gcggggcggt cggtccgctg agggatccca gggtcaaggc
      241 ggccgcgctc gcggccctct gcggcgagct cgagctgcac acggattatg tcgcggggga
      301 gatcgcggcc ggcggcgacg gggtgacgat gcgctacatc tgcgacggcg agcaccgctc
      361 ggggacgtgg gcgcgtgtgc tgctcgcggc gggacgcaag tcgttgctcg gagagctcgg
      421 gctggagcac gcgggggtgg tgctcgacgg gaaggggcga ccgcagggct tcgatgcgca
      481 gacgctgcgg atcggcgaga cgaacgtgtt cctcgcgggc gatgtgtcgg ggctgcggcc
      541 gctgttgcac gaggcctcgg acgaggggca catcgccggc ggcaacgcgg cgcgcctgcc
      601 cgatggcgtg cagccgcaac cgcgacgcac gaagctcggc gtggtgttca ccgaccccgg
      661 gatggcggtc gtcggcgacg gcttcgaggg cgtgaccccg gagacgcacg cggtcggcga
      721 ggtcgactgg agccgccagg ggcgcgcgcg ggtgctgcga aagaattatg gcgtgtcgag
      781 gatctacgcg acccgcagcg accgcaagct gcacggcgcg gagctggccg gacccggcgc
      841 ggagcacatg gcacacctgc tggcgtgggc ggtgcaacag gggctgacgg tcgacgacgc
      901 gctgcgcatg ccgttttatc acccggtgct ggaggaaggg ctgcggacgg cgttgcaggc
      961 gttgaaggcg gatctgggca aggtggtgtg agcggtcgaa ggcgggttga cgaggaataa
     1021 cgcgcgtgtt atatcaggtg cacgatgccg aaccaccgcc cccacgcgcc ccgctttacc
     1081 cgaaagaaaa agcttcatga agattcggtt cttcgaaacc aatcgcggcc aagcccctgt
     1141 ccgggagtac atcgactcgt tgcctacacg tgacgtggcc aagatcggtg ccgcgctaag
     1201 cgatatcgcc gaacacggac ttcgtgacgc ccttactcag cttcgtcaaa ttcggggcac
     1261 gctgtgggag atcaagatcc aaccccatcg tatcttttac gcggttgtcg aaggggacac
     1321 tctcgtgctc ctgcatgcgt acaggaagca gagtcagcgc gcccccaaga acgagattgc
     1381 gaccgctctc gggcgactga aggtgctcac cgagtgagag gggcagcatg actggtccac
     1441 gtaagaagtc gtctccaggt agcaagcgtg cgcatgtcgg cgccgatgtc atggcttatt
     1501 ttcgcgacct ggctgccgca gatccagagt tcgctacagc ctcccgggag gaggctgaca
     1561 agctgaagct ggcgcggcgc attcgagagc tgcgtgaggc gaaggggctg agccagacac
     1621 agctcgcgga gatgatcgag accaaacagc cgtcgatcgc acgcattgaa agcggtcggg
     1681 cgttaccccg gctggaggtg cttcagcgga tcgccgcagc gctcggcatg cgcctgacga
     1741 tcgaatttga aaaggtcccg gcaccacggc gcaagcgggc gaaggctgcg gtggtcgaac
     1801 atactgcgac ctggacgaac gacgaatcgc ggccctcggc tcgagatcat gcgcgatagc
     1861 tggtacctcg ccctcgtgct cgcgagttgc catgcggcac ccgcggcggc gccggtcgcc
     1921 gagacggtga aggccgacgc cggttcgttc acgatgcatt cgcgggcgct cggcgaggtc
     1981 cggcgcatca atatctatac acctccggga tacgcggatt ccggcctgcg gtatccggtg
     2041 ctgtatatgc ccgacggcgg gctggaagaa gacttcccgc atgtcaccga ggacatcgag
     2101 gcggccgtgc gcgcggggca gatgcgggcg atgatcgtcg tcgggatcga gaacaccgag
     2161 cggcgacgcg acatgaccgg gccgacgatg aacgaggaag ataaaaagat cgcgccgcgg
     2221 gtcggtggat cggcccagtt tcgcgcgttc atccgcgacg agctgatgcc ggagatcggc
     2281 cggcgcgtgc gcggcgatgg ggagacggcg atcgtcgggg agtcgctcgc ggggctgttc
     2341 gtgctcgaga cgttctttgc gcagcccgag ctgttcgaca cgtatgtcgc gctgagcccg
     2401 agcctgtggt ggaacgacaa ggcgctgctg cgtggcgcgg ccgctcgatt gcacgagcgg
     2461 ccacgctggc ccgggacttt atattttgcg acggccggcg acgacgatcc cgacggggac
     2521 ggcgaggcct tggccgcgat cctgcgcgcg gcggccccgg tcggcctgac gtggtcctat
     2581 cagaagcacc ccgacctgct gcacagcacg atctaccgcg gggcctcacc cgcggtgttt
     2641 cgccggctgt tcccgcctgt cgctgtgagg cgctgacgga ccgcgatcaa gctgcggatc
     2701 cggcccggtc ccaggcgagg tgcaggtgct cgtagccgaa gaacatcgcc ccgggggcgc
     2761 gctcgcgggg gcggtccggg gcgatgcgca ggcccggcag gcgctcgagc aggcgctcga
     2821 ggccgacgcg catctcgagg cgtgacagcg ccgcgccgat acaaaagtgg atcccgcggc
     2881 cgaacgagaa gtggcggtcc gcgttctcgc ggtgcatgtc gaggcggtcg gggtcgggga
     2941 agaccgcggg gtcgcggttg gccgcgggga tcagcagcag cagcgagctg ttggccggga
     3001 tcgtgacgtc accgagcttg acctcggtga gcgccgtgcg ttggttgacg agcaacggcg
     3061 tttgatgacg aatgctctcc tcgatgaatt gcggcagcag cccggggtcg gcgcgcacgg
     3121 ccgcgaggtc tgctggctca cgcagcaggg cgtcgagcgc gttgctgatc gcaaacaggg
     3181 tggtctcgtg cccgccgatg atcagctgca tcggcacggc gacgagctcg cgctccgaga
     3241 gccggtcgcc atcggccagc cgggcctgga tcagcagcga gagcacgtcg tcgccggggt
     3301 gcttcctgcg ctcggcgatc atgtcctgaa agtaccgctg gaaggccaaa aaaccgtggg
     3361 cagactcgac caggcgctcg agcgggtcag cgcaccagac caggcggatc cagtcacccg
     3421 accaggtctt gaggcgggtg atgtcggcgc gcgggacccc gagcatgtcg ccgatcacca
     3481 tcgcggtgaa cggaaaggcg aagcgctgga cgatgtcggt ctcgccgtcg gcgacaaagc
     3541 cgtcgatcag ctcgtcggcg aggccccgga tcgccggctc gcgcgcggcg atcgcctgcg
     3601 gcgagaagcc cttgatcagc aggctgcgca tgcgcgtgtg atcgggcggg tcagtggtca
     3661 ggagcgtcgg caaccacggg tcctggccgg cgaacacggc ctcgacctcg ggcgggagcg
     3721 ccgagtgcgg gctcatgatg tattttgaag agaagcgcgc cgggtcgcgg tagatcgcct
     3781 ggatgtcgtc cgggcgggtc accatccagc tgctcagctg cgcattgtaa aatgccgcgg
     3841 tcgcctggcg agccctggcg taaaatggat agggatccac catctgcgcc gggtcgtagg
     3901 ggttatgcgc ggcgaggaaa tccgggtttt ggatcgtggc ggacatcgtg aggaccacgg
     3961 tctaccagga ttattccgag atcacgatac ggatgatcga cactcgcgcg ggtgcacgcg
     4021 ccatgcgtgc cagcgcaggt cgaccaaacc ggctggcacg gcgtttcgag cccccttcgg
     4081 ggcgagccgg tcgtgaatcc gcgtagttcg tcgcggcggc gggtcgctca gcccggctcg
     4141 agcacgaagg ggtaggtgac ctcgacggag ccgccatcgg gcttcgggaa cagccagccc
     4201 tcgatcgccg cgctcatgca cgtcggcacc gcggcgtcct tcatgctgct ctcggcgacg
     4261 gcggtcttcg tgacggtgcc cttgtcgccg atcgtgaagt cgatgacgat cttgccgctg
     4321 gcctgggggt cttttgataa ggcctggttg taacaggcgc ggatctccga gatgtgggcc
     4381 ttgacgacgc cgctgatctg gtccttgtcg agctgaccct cggcggcgct ggccggggtg
     4441 gtgcaggtga gagcgacgaa gccgagcagc gtgtggatga tggtgttttt catggtgaac
     4501 ctcttgcctc gtccggtctc acggcggaca acgccggagg ttcccaagaa ttttatcaat
     4561 gcagcgaaaa ggcgctcaac gcccgggcga ggccgcgcgc gggcaggacc agggtccgac
     4621 cggcgcgagc gcctcagggc aggtcgcggc ggacgaacag cagctcgtcg ggggaccggc
     4681 gcgcgagcag gtgcagcagg ttggcctcga acacgagcac cgtggtgaac gacacgagca
     4741 gccacaggca cggctcctcg atcggcaggc cgttccacgc gcggatgaac acgccgatca
     4801 tcatctcgtc gcggtaggtc caccactggt aggggaacgc gatcatcgcc tcccaccagg
     4861 tgctgatcgt gaacatcacc agcgcggtga acgagaaggc gcgccagttg acgcggtgcc
     4921 ggacgaccgg gaacaccatg caggcgggca cgaaggcgac gagcacgagg aacgcgaagt
     4981 acgccgggaa gccgtcgggg accggcgagc acaggcgctt gtatgcgaag gcgacgccga
     5041 tcagcgccag cccgagcaac aacgcgcgcg gctcgaagcg gatccgggcg ccgcgcggcg
     5101 gcaggcgctc gcgcagcgaa cggccgtaga ggccgaacca gtgcaggtca caccagagat
     5161 agaggacgag ggtgaaggcg acgccgaaga agtagaacgc gagctcctcg acgggcacgt
     5221 tcgggaccat cgacatcgac gcgacctcga acccgtagag gttgatcccg agcgtcgcgc
     5281 cgtggttggg aaatttgaag aaggcgtcgc cgagcgcgac gtcgaggacc acgccgagcg
     5341 cggcgagggc ggcgacggtc cgccagaacg cgaggcgcgg caggtgaaag ccggggctgc
     5401 gctgcagcca taccaccaac acggtcagcg gcacgaccca cagggcgagc gaccaggtgt
     5461 atccgtaggg cgtcgggttg tcggaggtca tcacgaggac cgccggtgac tgcacggatt
     5521 gcagggtgat catcagcggg atcgccaggg cggcgaccat cgccaggacg gtgcgcgcgt
     5581 cgtcccgccg caccggaggt tgcaggtcca tcgaatgcat aagctcgctc ctttaataga
     5641 aatgtcatgg gcaagtggcg cgggtcatcc gaccagcgcg aaggcgatcc ggccggcgac
     5701 gtagatcgcc agggcctgca gcgcgccgag gccgacgacc ccgcgccacg tggtcgtggc
     5761 gacgcgcaac agcagcagcg gaatgacgag gccgagcatc gcctcggcga cgatcacgta
     5821 atgcgggacg gcgccgagga ccatcggcgt attttggtag tgccagaggt ccgcgtattt
     5881 ggcgagcagc tcgtgcgccg gaatgttgag cccgccgagc acggcgaggg cgacgcaggc
     5941 gcgcaggggg ccccagcgcg gcgccagcca gccggcgagc acgccgagct ggaccatcat
     6001 cacgacccag ccaaatggca tgtacagcgg cgtgacgccg acgtacggcc cgcctggcga
     6061 atatacgagg gtctcggtga cgacgacgcc gaagtggtcc gcgggcaatt caccgaagcc
     6121 gacgacgagc ccgaacagaa ggatcttcgc gagcacgacg tcgcgctcgc gccaggcgtg
     6181 cgcgaccagg acgccgagca gcgccgcggt cacgacgctg gtggtcagcc agccgagcga
     6241 gcatatggcc gagacgacct gcgcgagcag ggcataggcc attgtgcgta ggacgacaaa
     6301 caggcgccgg cggtcgcgtt ccatcgcccg agttctagga gacgggaccg cggcggtgga
     6361 cgtcctcgcg gcggcgacat cgtgacggcg gtcacgttcc gtgcga
//
LOCUS       DASWNJ010000438         1560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_467166, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000438 DASWNJ010000000
VERSION     DASWNJ010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467166"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VGB85_07530"
     CDS             <1..823
                     /locus_tag="VGB85_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133913.1"
                     /translation="RLKKQQDRVDQLKRQHAQEKEAQRRLDKLRRDAQDAKSGSKGKD
                     QQKRALERLRREASDASKKSQQSRRMRQARDNMEEAKSFLRKAGSGDQDQKRKQQMQR
                     FSKAAKGKRGEKGKGPTILVEGDVGDSQGEPMEMDSGDADGDGQGVGEGDGEGEGDAD
                     GDGDGQGDGQGQGQSNGAGQGDGMGQGTQDPLGDPAGLKVKTKQIKVNPKHGRGATRA
                     EVIRTASQEGFANTAYRDVYTDYKSFAQNSIDSEAVPAPQRRRIKRYFQMIQPRR"
     gene            893..>1560
                     /locus_tag="VGB85_07535"
     CDS             893..>1560
                     /locus_tag="VGB85_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133914.1"
                     /translation="MRSLKCSTSLALLLTACSGGGNDDTGSTTATTGGPTGDPGTDGS
                     ATDSPTSPDTPTSVASDPSMASATEPTTTNPSDPTATDPTATTDPTGPDPGELIEPGD
                     PGPGDVTFTIRADQDVHPIDPRIYGTNQPHDLDTLQRGIGLVRQGGNRMTAYNWENNA
                     SNAGADYMHQNDGYLESQLMVDGDIPGEVVRKATQDALDAGADMMVTIPICGYVAADK
                     HGNG"
BASE COUNT          327 a          562 c          523 g          148 t
ORIGIN      
        1 ccggctcaag aagcagcagg accgcgtcga ccagctcaag cgccagcacg cgcaggagaa
       61 ggaggcccag cggaggctcg ataaactccg ccgcgacgcc caggacgcca agtccggtag
      121 caagggcaag gaccagcaaa agcgcgcgct cgagcgcctg cgtcgcgagg cgagcgacgc
      181 ctccaagaag tcacagcagt cgcgccgcat gcgacaggcg cgggacaaca tggaggaggc
      241 caagagcttc ctgcgcaagg ccggcagcgg cgaccaggac cagaagcgca agcagcagat
      301 gcagcgcttt agtaaagccg ccaagggcaa gcgcggcgag aagggcaagg gcccgacgat
      361 cctcgtcgag ggcgacgtcg gcgacagcca gggcgagccg atggagatgg acagcggcga
      421 cgccgacggt gacggtcagg gtgtaggtga aggcgacggc gagggtgaag gcgacgccga
      481 cggcgacggc gacgggcaag gcgacggcca gggccagggt cagagcaacg gcgccggtca
      541 gggcgacggc atgggccagg gcacccagga cccgctcggt gaccccgccg gcctcaaggt
      601 caagaccaag cagatcaagg tcaacccgaa gcacggccgc ggcgcgacgc gtgcggaggt
      661 cattcgcacc gccagccagg agggctttgc gaacaccgcc taccgcgacg tctacaccga
      721 ctacaagagc ttcgcgcaga acagcatcga cagcgaggcc gtgcccgccc cgcagcggcg
      781 ccggatcaag cgctacttcc agatgatcca gccgcgccgc tgacacgcgc tcgcgcgcgc
      841 ctgagcgttc cgcgcgggtg cggctcgcat ggcgcttgag tatcctgaca cgatgcgtag
      901 tttaaagtgt tcgacctctc tcgcgctcct cctcaccgcc tgcagcggcg gcggcaacga
      961 tgacaccggt tcgaccaccg cgacgaccgg cggcccgacc ggtgatccgg gcacggacgg
     1021 cagcgccacc gacagcccca cgagccccga tacgccgacg agcgtcgcca gcgatccatc
     1081 gatggccagc gcgaccgagc cgaccacgac gaacccctcg gacccgaccg cgaccgaccc
     1141 gaccgcgacg accgacccga ccggccccga tcccggcgag ctgatcgagc ccggcgaccc
     1201 cggccccggc gacgtcacct tcacgatccg cgccgaccag gacgtgcacc cgatcgaccc
     1261 gcggatctac ggcaccaacc agccgcacga cctcgacact ctgcaacgcg gcatcggcct
     1321 ggtgcgtcag ggcggcaacc gcatgaccgc ctacaactgg gagaacaacg cctccaacgc
     1381 cggcgccgac tacatgcatc agaacgacgg ctacctcgag tcgcagctga tggtcgacgg
     1441 cgacatcccc ggcgaggtgg tccgcaaggc cacgcaggac gccctcgacg ccggcgccga
     1501 catgatggtg acgatcccga tctgcggcta cgtggccgcc gacaagcacg gcaacggcga
//
LOCUS       DASWNJ010000439         5230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_255911, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000439 DASWNJ010000000
VERSION     DASWNJ010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255911"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..679)
                     /locus_tag="VGB85_07540"
     CDS             complement(107..679)
                     /locus_tag="VGB85_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133915.1"
                     /translation="MLTTLTLSGAVSTAVAARYIVVQRRRKLRAAEVEALSEDTPAGS
                     AVHLGPALAHVVTQARVVRLMLATPLERGEPPVLSETPWGRRARCDQYDLALGDARRA
                     LWEWLLLFRRLGERDRHFLLALGVSVAPFYDTLFRPGVFDRTDDPWDENVYPEAPDHE
                     RVFGALRRTMHDLRSFETALLSAVHDPYRR"
     gene            complement(835..1320)
                     /locus_tag="VGB85_07545"
     CDS             complement(835..1320)
                     /locus_tag="VGB85_07545"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY0133916.1"
                     /translation="MLQTGDQAPDFTLPNHKRERVSLESLRGKTVVLAFFPAAFTGVC
                     EKEMCSFRDSLTALNDLNATVLGVSVDAPFANAAFAERNQLNFPLLSDYNREAVKAFG
                     IAHDDFAGMPGYTAAKRSVFVIDAAGKVAYAWVGPNPGVEPNYDEVKQAVTELDASSK
                     A"
     gene            complement(1404..2846)
                     /locus_tag="VGB85_07550"
     CDS             complement(1404..2846)
                     /locus_tag="VGB85_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133917.1"
                     /translation="MLSDDATTELRSRSRDQRAPSSPDEVAWSIPPLVLGRTAQAAVG
                     APTPHLPVGYRLGEHVITEPRGRDGCFELHVVTHAERGSLHMARVLRREHADLAPAML
                     EAVRKIQDHPQRNLREVLEAGWTDEPTPRAFVVHERLVGRSLASLVHGRSEIEWPLVV
                     AIGVQCAEALEALHELGLAHTHLGPDSAALMHVAGDQFRVVLGGLEHAQAIVPGSDAA
                     EEPRVRDDLLALGEMLNALAAGCCATTSAPMPEALTALLARMVDPRPAMRPMTASEIH
                     EQLAAVHDHVDPQLSSVRMLGEAFRKIACDDFEVSVELDEPAEPARARPVAPVPVIAY
                     VRPAAPRWPSLLLVAVVAAVAFAAGASLPDRPGPTTARTLSQDVHDDREEAPAPTRRA
                     LPRLRAQEPTPEPAPAVVLLPRQPRVARVQPRPAAETADSADTTPASAPAPAEPAAVE
                     PAADDESPDPDPLLPVPVQDEVPAVVDASS"
     gene            complement(3033..4613)
                     /locus_tag="VGB85_07555"
     CDS             complement(3033..4613)
                     /locus_tag="VGB85_07555"
                     /inference="COORDINATES: protein motif:HMM:NF027442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HEY0133918.1"
                     /translation="MFMRRLARSLPVHAVLLAACSTDPPAGGDTGSSDTGSSDTGGST
                     ATSSPTDPTDPTSTSGPTDPTSSSTDPGTSTTTSTTTTDTDTTTGEPGLSCPLDGQPG
                     VEQLMLAPLLFGEGADASAAPSCTYLNPERGFHQPVSLRSVGADTLEQTVAAGMSVIY
                     GQVLIPEYRDKPLDDALLDEIHAGFDLVRAHGMKVVPRFHYSNAGDEPDAPLAIILGH
                     IDQLAPVLQEHADVILTVQAGFIGAWGEWHSSQNGLDAPGPRKQVLDALLAALPPDRT
                     ITVRRPSFKQAAYGGPVTEATAHDGSALARIGHLNDCFLASDDDFGTYEDAAEKDYAI
                     ADSPFVPVGGETCAVNPPRSECPSALDELALLHWTHLNVGYHPDVLDAWKEDGCYTEV
                     ACRLGYRLAVKQLRYAHAATPGGVLPVSFDIHNDGFAAPVNARPLVLVLDGPSRVEVP
                     VQFDLRDLLPGTTSTVCVDAALPADSLAGFYRIGLRLPDPAPTLADDPRQAIRLGNDD
                     VVEWSDGINWFDAMIAVE"
     gene            complement(4655..>5230)
                     /locus_tag="VGB85_07560"
     CDS             complement(4655..>5230)
                     /locus_tag="VGB85_07560"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0133919.1"
                     /translation="IYDMGVYPINAVRYLFREEPIEVSAVVSSSGDARFEGVDETTTA
                     ILRFASGRVAQLTVSLGAAAVGWYHVIGTEGDLHGEPIYGYTGSLRHRITREGKVREV
                     EFARRDQFAPELVMFSRCVLEGRDPEPCGFEGLADIRIVEAILRSARDHKAVALPPWS
                     RPARPDMRQELQKPPVDAPPEPIHAPSPSIR"
BASE COUNT          721 a         1646 c         2145 g          718 t
ORIGIN      
        1 gttctcgatc tcggcgacgc tctccggggt gccgtccggg gcgtggacga tcagcgtcgg
       61 gccgttctcg cagcaggtcg aggtgccgtg aaacgtcagc ggcggctcag cggcggtaag
      121 ggtcgtgcac tgcgctgagc agggcggtct cgaagctgcg caggtcgtgc atggtgcggc
      181 gcagggcccc gaacacgcgc tcgtggtcgg gggcctcggg gtagacgttc tcgtcccagg
      241 ggtcgtcggt gcggtcgaag acgccggggc gaaacagggt gtcgtagaat ggtgcgaccg
      301 agacgccgag cgcgagcaag aagtggcggt cgcgctcgcc gaggcggcga aacagcagca
      361 gccactccca cagggcccgg cgcgcgtcgc cgagggcgag gtcgtactgg tcgcagcggg
      421 cccgccggcc ccacggggtc tcggacagga ccgggggctc gccgcgctcg agcggggtgg
      481 cgagcatcag gcgcacgacg cgggcctggg tgacgacgtg ggcgagggcg gggccgaggt
      541 gcacggcgct gccggcgggg gtgtcctcgc tcagcgcctc gacctcggcg gcgcgcagct
      601 tacgccggcg ctgcacgacg atgtagcgcg ccgcgacggc ggtggagacg gctccggaca
      661 gcgtcagcgt cgtgagcaac gtttttaaag atgcgcgagg acccggggcc gctcaagcgc
      721 cggcctcgtg agccgggcat gtatgtttcg cgggcggttt cgcggcgcga agtgcctgtc
      781 ccgaaggaca ggtgaggtcg cggccccccg gtgggcggcg cgcgacggag gcgctcaggc
      841 cttgctgctg gcgtcgagct cggtgacggc ctgcttgacc tcgtcgtagt tgggctcgac
      901 gccggggttg gggccgaccc acgcgtaggc gaccttgccg gcggcgtcga tgacgaacac
      961 cgagcgcttg gccgccgtgt agccgggcat gccggcgaag tcgtcgtggg cgatgccgaa
     1021 ggccttgacg gcctcgcggt tgtagtcgga gagcaggggg aagttgagct ggttgcgctc
     1081 ggcgaacgcg gcgttggcga agggggcgtc gacgctgacg ccgagcacgg tcgcgttgag
     1141 gtcgttgagg gcggtcagcg agtcacggaa ggagcacatc tccttctcgc agacgccggt
     1201 gaaggcggcc gggaagaacg cgaggacgac cgtcttgccg cgcaggctct cgagcgagac
     1261 gcgctcgcgc ttgtgattgg ggagggtgaa gtcgggggcc tggtcgccag tttgcagcat
     1321 ggagcatgcg taccgaaggc ccgcgggggc cgcaaggccc aaagagtgac gcgtgactcg
     1381 gggcgtgact cgggcgtggc ggctcaggac gacgcgtcga cgaccgccgg gacctcgtcc
     1441 tggacgggga ccggcagcag cgggtcgggg tccggtgact cgtcgtcggc cgcgggctcg
     1501 acggccgcgg gctcggcggg ggccggcgcg ctcgcaggcg tggtgtcggc gctgtcggcg
     1561 gtctcggcgg cgggacgcgg ctgtacgcgg gcgacgcgag gctggcgggg cagcagcacg
     1621 accgcgggcg cgggctcggg tgtcggctcc tgggcccgga gccgcggcag agcgcgccgg
     1681 gtcggcgcgg gggcctcttc gcggtcgtcg tggacgtcct gcgagagcgt gcgcgcggtc
     1741 gtggggcctg gccgatcggg cagcgacgcg ccggccgcga aggcgacggc ggcgacgacc
     1801 gccacgagca gcagcgacgg ccagcgcggc gcggcggggc gcacgtaggc gatcacgggg
     1861 acgggcgcga cggggcgcgc gcgcgcgggc tcggcgggct cgtcgagctc gaccgagacc
     1921 tcgaagtcgt cgcacgcgat cttgcgaaat gcctcgccga gcatgcgcac gctcgagagc
     1981 tgcgggtcga cgtgatcgtg gacggcggcc agctgctcgt ggatctcgct ggcggtcatc
     2041 ggacgcatcg cgggccgcgg atcgaccatg cgcgcgagca gggcggtcag cgcctcgggc
     2101 atcggggcgg aggtggtcgc gcagcagccg gcggccagag cgttcagcat ctcgccgagc
     2161 gcgagcaggt cgtcgcgcac gcgcggctcc tcggcggcgt cggacccggg cacgatcgcc
     2221 tgggcgtgct cgaggccgcc gagcacgacg cggaactggt cgccggcgac gtgcatcagc
     2281 gcggcgctgt ccggcccgag gtgcgtgtgg gccagcccga gctcgtgcag ggcctcgagc
     2341 gcctcggcgc actgcacacc gatagcgacg accaggggcc actcgatctc gctgcgcccg
     2401 tggaccagcg aggcgaggct gcggccgacg aggcgctcgt gcacgacgaa ggcgcggggc
     2461 gtcggctcgt cggtccagcc ggcctcgagc acctcgcgga ggttacgctg cgggtggtcc
     2521 tggatcttcc gcacggcctc cagcatcgcc ggggcgaggt cggcgtgctc gcgccggagc
     2581 acgcgggcca tgtgcagcga gccgcgctcg gcgtgggtga cgacgtggag ctcgaagcag
     2641 ccgtcgcgcc cgcgcggctc ggtgatcacg tgctcgccga gtcgatagcc gaccgggagg
     2701 tgcggcgtgg gcgcgccgac ggcggcctgc gccgtgcgtc cgaggaccag cggggggatg
     2761 ctccacgcga cctcgtcggg cgaggacggg gcgcgctggt cacgggacct ggaacgaagc
     2821 tcggtcgtcg cgtcgtcgga cagcatggga acctccgggt gagttcgccg cacatgttaa
     2881 tagacgtgtg tgccttgtgc ccggacctac cgaccggaac acaaggcacc cacggagcgg
     2941 ccgctctttg gtcagtgccc gggaggcggc gggacgatca ctcgatccga agaattcttg
     3001 cgggggccgg ccccgcaaaa cctagtaatc gatcattcca cggcgatcat ggcgtcgaac
     3061 cagttgatcc cgtccgacca ttcgacgaca tcgtcgttgc cgaggcggat cgcctgacgc
     3121 gggtcgtcgg cgagggtcgg ggcgggatcc ggcaggcgca ggccgatccg gtagaagccg
     3181 gccagcgagt ccgcaggcag cgctgcatcg acgcagacgg tgctggtggt ccctggcagt
     3241 agatcgcgca ggtcgaattg gacgggaacc tcgacccgcg acgggccgtc gagcaccagc
     3301 accagcgggc gcgcgttgac cggggcggcg aagccgtcgt tgtggatgtc gaaggagacc
     3361 ggcagcacgc cgcccggggt ggcagcatgg gcgtagcgca gctgcttgac ggcgaggcgg
     3421 tacccgagcc gacatgcgac ctcggtgtaa cagccatcct ctttccaggc gtcgagcacg
     3481 tcggggtggt agccgacgtt gaggtgggtc cagtgcagca gggccagctc gtcgagcgcg
     3541 gacgggcact cggagcgggg aggattgacc gcgcaggtct ccccgccgac cgggacgaac
     3601 ggggagtcgg cgatcgcgta gtccttctcg gcggcgtcct cgtaggtgcc gaagtcgtcg
     3661 tcggaggcga ggaagcagtc gttgaggtgg ccgatgcgcg cgagcgcgct gccgtcgtgg
     3721 gcggtggcct cggtgacggg gccgccgtag gccgcctgct tgaaggaggg ccggcgcacg
     3781 gtgatcgtgc ggtccggcgg cagggcggcc agcagcgcgt ccaggacctg cttgcgcgga
     3841 ccgggggcgt cgaggccgtt ctgcgaggag tgccactcgc cccaggcgcc gatgaagccg
     3901 gcctgcaccg tgaggatgac gtcggcgtgc tcctgcagca ccggcgcgag ctggtcgatg
     3961 tggccgagga taatcgcgag cggggcgtcg ggctcgtcgc cggcgttgga gtagtgaaag
     4021 cgcgggacga ccttcatgcc gtgcgcgcgc acgaggtcga agccggcgtg gatctcgtcg
     4081 agcagggcgt cgtcgagcgg cttgtcgcgg tactcgggga tcaacacctg accgtagatc
     4141 accgacatgc cggcggcgac cgtctgctcg agggtgtcgg cgccgaccga gcgcaggctg
     4201 acaggctgat gaaagccgcg ctcggggttg aggtacgtgc acgacggcgc ggccgaggcg
     4261 tcggcgccct cgccgaacag caaaggcgcc agcatgagct gctccacgcc cggctgaccg
     4321 tcgagcggac agctcaggcc gggctcgccg gtggtcgtgt cggtgtcggt cgtggtggtg
     4381 gaggtcgtgg tactggtacc cgggtcggtc gagctcgagg tcgggtcggt cggacccgag
     4441 gtggaggtcg ggtcggtcgg gtcggtcggg ctcgaggtcg cggtcgagcc cccggtgtcg
     4501 ctgctgccgg tgtcgctgct gccggtgtcg ccgccagcgg gcgggtcggt gctgcaagcg
     4561 gcgagcagga cggcgtggac ggggaggcta cgggccaggc gtcgcatgaa catcggccga
     4621 cgatagccga gtgatgcgag cgcaggcgcg cgcgtcagcg gatgctcggc gagggcgcgt
     4681 ggatcggctc gggcggggcg tcgacgggcg gcttttgcag ctcctggcgc atgtccggcc
     4741 gcgcgggtcg cgaccacggc gggagcgcga cggccttgtg gtcgcgcgcg gagcggagga
     4801 tcgcctcgac gatgcggatg tcggccaggc cctcgaagcc gcagggctcg gggtcgcggc
     4861 cctcgagcac gcagcgcgag aacatcacga gctcgggggc gaactgatcg cggcgagcga
     4921 actccacctc gcggaccttg ccctcgcggg tgatgcggtg gcgaagcgag ccggtgtagc
     4981 cgtagatcgg ctcgccgtgc aggtcgccct cggtcccgat cacgtggtac cagccgaccg
     5041 cggcggcgcc gaggctgacg gtcagctggg cgacgcgacc gctggcgaac cggaggatcg
     5101 cggtggtcgt ctcgtcgacg ccctcgaagc gcgcgtcgcc gctgctcgag acgaccgcgg
     5161 acacctcgat cggctcctcg cggaacaggt agcgcacggc gttgatcggg tagaccccca
     5221 tgtcgtagat
//
LOCUS       DASWNJ010000440         2714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_323168, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000440 DASWNJ010000000
VERSION     DASWNJ010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323168"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VGB85_07565"
     CDS             <1..364
                     /locus_tag="VGB85_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0133920.1"
                     /translation="STPLLPVADGVIVLPLIGSLDPDRAEQVMQALLAGVVEHRATTA
                     ILDITGLQTVDTAAAEALMRTARAIRLLGAEAIVTGVRPAVAQTLVALGTELGGLVVL
                     GDLKAGIRHALARRNARA"
     gene            complement(378..812)
                     /locus_tag="VGB85_07570"
     CDS             complement(378..812)
                     /locus_tag="VGB85_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133921.1"
                     /translation="MNGSRDYLLEVIERAGEALAAMATGRTDLRAREEETDVELAFDR
                     EFGHLHLHLARVDATSAALLLRPPRRVRLYALMLVRLAATRDEAGAEALVRRALELLL
                     AADAVDPGGREPALLAMLAASVDHTRLEPFARAALAPVSGGM"
     gene            906..1508
                     /locus_tag="VGB85_07575"
     CDS             906..1508
                     /locus_tag="VGB85_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133922.1"
                     /translation="MPPTTRWILLAALAGCDGAPEAPAPGPGPDCQALIAAGERARAC
                     DPAIDDLLAELRTRPDERRCRTAARLLLAPPGPSQGRVVSVYERPPALGEAPLEPAER
                     DGLLALPLPGRLVLAPDLAPKPGLPATTATLAGAALQVDANGRLRGHAAPGAHTLEVR
                     HANDRAVACVTLTACETLALTAHGASLAPHPAVRTGPCEP"
     gene            complement(1514..2215)
                     /locus_tag="VGB85_07580"
     CDS             complement(1514..2215)
                     /locus_tag="VGB85_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133923.1"
                     /translation="MTLCRTFTSSTLLALSLVVCAGVSLPGCGDKGGYRGGKKTKARQ
                     WLDDPSSGQKEGDFIKFPQLDLVFEIPDTLYVFKNCAEASHSPEGDEKWVPIISCASS
                     GAAADPDGVGEAAAPDSDAIALTFYLAPKGRPIDERAVAYYNNAYQESGLKVEELAFN
                     DDYFYKTGIFAKLQVMDESGTNAIREIQQFMWPVHDVVFIARMEYPYGDSRAVQQDWK
                     AIMPYFKTAGDDVPK"
     gene            complement(2325..>2714)
                     /locus_tag="VGB85_07585"
     CDS             complement(2325..>2714)
                     /locus_tag="VGB85_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEY0133924.1"
                     /translation="FVRVNFANGDMVGHTGDLNATILAVEAVDLCIGRILAALRVRKG
                     VAVITADHGNADQMFDMRPDGALQIRTSHTLNRVPFAIIDPREPGGGPRLAPPPSAGL
                     SNVAATCLELLGFSAPEHYRGSLLASR"
BASE COUNT          352 a          997 c          999 g          366 t
ORIGIN      
        1 gtcgacgccg ctgctgccgg tcgccgacgg cgtgatcgtg ctgccgctga tcggctcgct
       61 cgaccccgac cgcgccgagc aggtgatgca ggccctgctc gcaggtgtcg tcgagcaccg
      121 cgcgaccacg gcgatcctcg acatcaccgg cctgcagacc gtcgacaccg cggccgcgga
      181 ggcgctcatg cgcaccgccc gggccatccg cctgctcggc gccgaggcga tcgtcaccgg
      241 cgtacggccc gcggtcgccc agaccctcgt cgccctcggc accgagctcg gcggcctcgt
      301 cgtcctcggc gacctcaagg ccggcatccg ccacgccctc gcgcgccgca acgcccgggc
      361 ctgaccaaaa gaccaggtca catgcccccg gagacaggcg ccagcgcggc gcgggcgaac
      421 ggctcgaggc gcgtgtggtc gacgctggcc gccagcatcg ccagcagcgc gggctcgcga
      481 ccgccagggt cgaccgcgtc ggcggcgagc agcagctcga gggcgcggcg caccagggcc
      541 tcggcgccgg cctcgtcccg cgtcgcggcc agccggacga gcatcagcgc gtacagccgg
      601 acccgtcgcg gcggtcgcag cagcagcgcg gcggaggtgg cgtcgacgcg tgcgaggtgc
      661 aggtgcaggt ggccgaactc gcggtcgaag gcgagctcga cgtcggtctc ctcctcgcgc
      721 gccctcaggt cagtgcgccc ggtggccatc gccgcgagcg cctccccggc ccgctcgatc
      781 acctcgagca agtagtcccg cgacccgttc atgctcccgt tctagcggcc cctggcgtcg
      841 atctgacgcg tccttcgggg gcagcggatt gacgcgcgcg cgtcacgtgc gacattgctg
      901 cctgcatgcc accgacgacc cgatggatct tgctcgccgc gctggctggc tgcgacggtg
      961 cgccggaggc ccccgcgccc ggccccggcc ccgactgcca ggcgttgatc gccgccggtg
     1021 agcgagcgcg ggcctgcgat ccggcgatcg acgacttgct cgcggagctg cgcacgcggc
     1081 ccgacgagcg ccgctgtcgc acggcggcgc ggctgttgct cgcgccgccc gggccgtcgc
     1141 agggccgcgt cgtcagcgtg tacgagcggc cgcccgcgct cggcgaggcg ccgctggagc
     1201 cggcggagcg cgacggcctg ctcgcgttgc cgctgccggg gcgcctggtg ctcgcgcccg
     1261 acctggcccc gaagccaggt ctgccggcga ccacggcgac gctcgcgggc gcggccttgc
     1321 aggtcgacgc gaacggccgg ctacgcggcc acgcggcccc cggcgcccac acgctcgagg
     1381 tccggcacgc caacgaccgc gcggtcgcct gcgtgacgct gacggcctgc gagacgctcg
     1441 ccctgaccgc gcacggcgcc tcgctggcgc cgcacccggc cgtccgcacg ggcccctgcg
     1501 agccttgagc gcctcacttc gggacgtcgt cgccggccgt cttgaagtac ggcatgatcg
     1561 ccttccagtc ctgctggacg gcccggctgt cgccgtacgg gtactccatg cgggcgatga
     1621 agacgacgtc gtggaccggc cacatgaact gctggatctc gcggatcgcg ttggtgccgc
     1681 tctcgtccat cacctgcagc ttggcgaaga tgccggtctt gtaaaaatag tcgtcgttga
     1741 aggcgagctc ctcgaccttg aggcccgact cctggtacgc gttgttgtag taggcgaccg
     1801 cgcgctcgtc gatcgggcgg cccttgggcg ccaggtagaa ggtcagcgcg atcgcgtcgc
     1861 tgtcgggcgc cgccgcctcg ccgaccccgt cggggtccgc cgccgccccg gacgaggcgc
     1921 agctgatgat cgggacccac ttctcgtcgc cctcgggcga gtgcgaggcc tcggcgcagt
     1981 tcttgaacac gtacagcgtg tcggggatct cgaagaccag gtcgagctgc gggaacttga
     2041 tgaagtcccc ctccttctgg ccgctcgacg ggtcgtcgag ccactggcgc gccttggtct
     2101 tcttgccgcc gcgataacca cccttgtcgc cgcagcccgg cagggacacc ccggcgcaga
     2161 cgaccagcga gagggcaagg agcgtggaac tggtaaaggt gcggcagagc gtcatcggcg
     2221 cggacattag caaagccggc ccgccacggg aagcagccgc gcgcgtcgcc ggccggcagg
     2281 gtccgccgcg gcgggcgcgc atacgcgcga ccccggagcg gccctcagcg gctcgccagg
     2341 aggctgccgc gatagtgttc cggcgcggag aagccgagca gctcgaggca ggtggcggcg
     2401 acgttgctca ggccggcgga cggcggcggg gccaggcgcg ggccgccacc gggctcacgg
     2461 gggtcgatga tggcgaaggg cacgcgattg agggtgtggc tggtgcggat ctggagggcg
     2521 ccgtcggggc gcatgtcgaa catctggtcg gcgttgccgt ggtcggcggt gatgacggcg
     2581 acgcccttgc gcacgcgcag cgcggcgagg atccggccga tgcacaggtc gaccgcctcg
     2641 acggcgagga tcgtcgcgtt gaggtcgccg gtgtggccga ccatgtcgcc gttggcgaag
     2701 ttgacgcgca cgaa
//
LOCUS       DASWNJ010000441         1661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_113601, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000441 DASWNJ010000000
VERSION     DASWNJ010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113601"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="VGB85_07590"
     CDS             <1..1285
                     /locus_tag="VGB85_07590"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY0133925.1"
                     /translation="EGFTPQYESTVSQNLWDAGAGMLGKLNMDQFAMGSSNETSAFGG
                     VASPYKGPEILDTDELLLNHLKAEDVVAFEATYIKCGNRFYRKLAPGGSSGGSSAAIA
                     ARLCPGATGTDTGGSIRQPAAFTGIAGIKPTYGRCSRWGTIAFASSLDQAGPMARDVR
                     DCAIMLEAMAGFDAKDATSLQLDVPAWEAGLNPSLAGKRIGIPREYRVDDMPPEIDAL
                     WQQGIAWMRDAGAEIVEVSLPHTKYALPAYYIIAPAEASSNLARYDGVRYGQRELPEG
                     AGLQDMYAATRAAGFGDEVKRRIKIGTYGLSAGFYDAYYTQASKVRTLIARDFERAWE
                     SCDLLLTPTAPSAAFALGEKSDDPLAMYLNDVFTVPSSLAGLPAMSVPGGLDANGLPL
                     GLQIIGRPLDEQGVLNAGLAIEQRASFAARPEAWW"
     gene            1335..1622
                     /locus_tag="VGB85_07595"
     CDS             1335..1622
                     /locus_tag="VGB85_07595"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY0133926.1"
                     /translation="MNAFAKVETGTMTSKGQVLIPKAMRDAAGLVPGRPLKVMLDEAG
                     KVVLAPIGYGPEDAEERVRLVRAGLEAIAGKYRDGRGTDAIMRELRGDYEP"
BASE COUNT          286 a          524 c          583 g          268 t
ORIGIN      
        1 cgagggtttc acgccgcagt acgagagcac cgtctcgcag aacctgtggg acgcgggcgc
       61 ggggatgctc ggcaagctta atatggacca gttcgcgatg ggctcgtcga acgagacatc
      121 cgcgttcggc ggtgtcgcaa gcccgtacaa gggtccggaa atcctagata ctgacgagct
      181 gcttcttaac cacctgaagg ctgaagatgt ggtcgctttc gaggcgactt acatcaagtg
      241 cggcaatcgc ttttatagaa agctcgcgcc cggcggatcg tcaggcggat cgtcggcggc
      301 gatcgccgcg cggctgtgcc cgggcgcgac cgggaccgac accggcggct cgatccgtca
      361 gccagccgcg ttcaccggca tcgccgggat caagccgacc tacggccgct gctcgcgctg
      421 ggggacgatc gcctttgcct cttcactcga ccaggcgggg ccgatggcgc gcgacgtgcg
      481 cgattgcgcg atcatgctcg aggcgatggc cggtttcgac gccaaggacg cgacctctct
      541 ccagctcgac gtgccggcgt gggaggcggg gctgaacccg agcctcgcgg gcaagcgcat
      601 cggtatcccg cgcgagtacc gcgtcgacga catgccgccc gagatcgacg cgctgtggca
      661 gcaggggatc gcgtggatgc gcgatgcggg cgcggagatc gtcgaggtct cgctgccgca
      721 cacgaaatac gcgctgcccg cctattacat catcgcaccg gcggaggcgt cgtcgaacct
      781 cgcgcggtat gacggcgtgc gctatggcca gcgcgagctg cccgagggtg cggggctgca
      841 ggacatgtac gcggcgacgc gcgccgctgg cttcggcgac gaggtcaagc gccgcatcaa
      901 gatcggcacc tatgggctct cggccgggtt ctacgacgcg tattacaccc aagcatcgaa
      961 ggtccgtacg ctgatcgcac gggacttcga gcgcgcatgg gagagttgcg acctgctgct
     1021 caccccgacc gcgccgagcg ccgccttcgc gctgggcgag aagtcggacg acccgctggc
     1081 gatgtacctc aacgacgtgt tcaccgtgcc gtcgagcctc gccgggctgc cggcgatgag
     1141 cgtgcccggc gggctcgacg ccaatggcct gccgctcggc ctgcagatca tcggccggcc
     1201 gctcgatgag cagggcgtgc tgaacgcagg actcgcgatc gagcagcgcg caagcttcgc
     1261 ggcgcgacct gaagcgtggt ggtgattgcc ggctccgctt gtaggtgtta cctcggtaac
     1321 actggagatt agccatgaac gcgtttgcta aggtcgagac gggtaccatg accagcaaag
     1381 gtcaggtgct gatccccaaa gcgatgcgcg atgccgccgg cctcgtaccc ggacggccac
     1441 tcaaagtcat gctggacgag gcgggcaagg tcgtgctggc gccgatcggc tacggtccag
     1501 aggacgccga ggagcgtgtg cgcctcgtcc gggcggggct ggaggcgatt gccggcaagt
     1561 accgcgatgg ccgtggcacc gatgcgatca tgcgggagct gcgcggcgac tacgaaccgt
     1621 gatactgatc gactcgaacg tcatcatcga tgtgatcgag g
//
LOCUS       DASWNJ010000442         5358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_323190, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000442 DASWNJ010000000
VERSION     DASWNJ010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323190"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="VGB85_07600"
     CDS             complement(<1..1317)
                     /locus_tag="VGB85_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0133927.1"
                     /translation="MTLADPTMSTPSPSRLFEKHSPVTGERLGEYAIAGQDAVDAAVA
                     RARTAFPGWRDTPLEVRLEALNRGRHVLAEHGDAYARKIAADTGKPIIEALMFELAVI
                     PLFLDHYLKRAPKLLTRRKVPTPLALPGKTSYIEHFPRGVIGIISPWNFPFQLSVLPM
                     ISALIGGNTVVLKPSEVTPISGEVVADFFNRIGLPPGVVEVVQGDGSTGAALCRANVD
                     MLFFTGSVVTGRKVMAAAAEKPIPVELELGGKDAFIVCADANLERAAEAAVFGAMANC
                     GQACVSVERVFVVDSVHDRFVELVKRKLAKLKVGGPDDHADMGPMTFAKQLETVQRHV
                     EAAQQAGAKVLSGGTRLDRPGLFFPPTLLTDIRPDMEIYREETFGPVLPVIRVRDEEE
                     AVRLANASEFGLAGSVWTQDIDKGMRLASRLESGQVMVNDVFSSVGH"
     gene            1427..2269
                     /locus_tag="VGB85_07605"
     CDS             1427..2269
                     /locus_tag="VGB85_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HEY0133928.1"
                     /translation="MTTGSPDELYRRYLGILGWVGDPGLHELVSSHLIKVPYENVSTL
                     LRSAEPAGATTLTPIAEFLENIAERDLGGNGYSIPIHFASLLQFLDYEVELLGADIGG
                     AARAHAVCRVRSEDTDYLVDIGHGAPFYQPIPLDTLPVHVPHGDYQYVIDRHASGPGA
                     IEVTTLRTGTRVQSYVAKLPALGPEGFAPAIAQARATDARTLRTLRISRFFASRVLEI
                     FDNHLVTTHHNITTVTPIRDIEELETIVREQLQLVRCPVREAVEILRSRGVDIFANSA
                     PPTP"
     gene            2285..3322
                     /locus_tag="VGB85_07610"
     CDS             2285..3322
                     /locus_tag="VGB85_07610"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY0133929.1"
                     /translation="MPSLARTLQSPWFILLLAACGQPPAPAGPEATTPAPVREVAVTF
                     DDLVIGGRILELPRMRTMTADLLRTITAEGVPAVGFVNEAKLCDCDERSGRIEVLRMW
                     AAAGLELANHTYSHPSLTCTPLADYQADVLRGEPVTRALMAEHGKPFRWFRHPYLHTG
                     PTLEVRAAFEPWLAERGYTVAPVTLDNSDWMFNFVYTDAKTRGDVALMRRVGEAFIAY
                     VDEVMAFYESAEQALFARPIRHVLLLHANELNADWFDDLAALLRRRGYRFITLEAALQ
                     DPAYREPDRYAGPAGVSWMYRWDFTRGREQIDWSSEPEPPAFVRELYDLAQRGPPRDQ
                     NSIPTPTVQVP"
     gene            complement(3283..3651)
                     /locus_tag="VGB85_07615"
     CDS             complement(3283..3651)
                     /locus_tag="VGB85_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133930.1"
                     /translation="MTRCDNSPPPTGAGLIAGGIIALVAGAVLIPTGVVQLRRGATTT
                     EEEEDALFTGNGLQTASGAAALIFGLVGAIGGAAMLGYGVQRRREFTDWQRRHSSFRV
                     APSFARTRHGTWTVGVGIEF"
     gene            complement(3906..5102)
                     /gene="purT"
                     /locus_tag="VGB85_07620"
     CDS             complement(3906..5102)
                     /gene="purT"
                     /locus_tag="VGB85_07620"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF006766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HEY0133931.1"
                     /translation="MDLELGTPLSPSATRILLLGSGELGREVAIEAMRLGIEVIACDR
                     YRDAPAMQIAHRSHVFDMQDRDAVRAVVGREKPHFIVPEVEAIATDALVELEAEGWTV
                     IPTARATRLTMDREGIRRLAAETLGLPTSPFAFADTLEQVEEAVAKIGLPCVIKPVMS
                     SSGKGQSTLRDRADLPAAWDYALAGARGKSARVIVEGFVRFDSEITLLTIRTREEGTL
                     FCPPIGHRQERGDYMESWQPHAMSEAALQKAQAQAEQVTGALGGFGLFGVEFFLVGDD
                     VIFSEVSPRPHDTGMVTMATQDLSEFELHVRAILGLPIVRVIQRAPGASSVILAERAG
                     HNPRFLGLGRALQDPRARVRLFAKPDTRKYRRMGVVLTYGETVEDARSRANAAARCIE
                     VVVDAG"
BASE COUNT          792 a         2027 c         1777 g          762 t
ORIGIN      
        1 gtggccgacc gagctaaaca cgtcgttgac catcacctgg cccgactcga ggcgcgacgc
       61 cagccgcatg cccttgtcga tgtcctgcgt ccacacgctg cccgcgagcc cgaactcgct
      121 ggcgttggcg aggcgcaccg cctcctcctc gtcgcgcacg cggatcaccg gcagcaccgg
      181 cccgaaggtc tcctcgcggt agatctccat gtcggggcgg atgtcggtca acagcgtcgg
      241 cgggaagaac aaccccgggc ggtcgagccg cgtgccgccc gacagcacct tcgcgccggc
      301 ctgctgcgcc gcctcgacgt ggcgctgcac cgtctccagc tgcttcgcaa aggtcatcgg
      361 gcccatgtcg gcgtggtcgt ccgggccgcc gaccttgagc ttggcgagct tgcgcttgac
      421 cagctcgacg aagcggtcgt gcaccgagtc gaccacgaac acccgctcga ccgacacgca
      481 ggcctggccg cagttggcca tcgcgccgaa caccgcggcc tcggccgcgc gctcgaggtt
      541 ggcgtcggcg cagacgatga acgcgtcctt gcccccgagc tcgagctcga ccgggatcgg
      601 cttctccgcg gccgcggcca tcaccttgcg gccggtgacc accgagccgg tgaagaacag
      661 catgtcgacg ttcgcccggc acagcgccgc gccggtgctg ccgtcgccct gcacgacctc
      721 gacgacgccc ggcggcaggc cgatgcggtt gaagaagtcc gcgacgacct cgcccgagat
      781 cggcgtcacc tccgagggct tgagcaccac ggtgttgccg ccgatcagcg cagagatcat
      841 cggcaggaca gacagctgga acgggaagtt ccacggcgaa atgatgccga tgaccccgcg
      901 cgggaagtgc tcgatgtagc tggtcttgcc cggcagcgcg agcggcgtcg gcaccttgcg
      961 gcgggtcagc agcttgggcg cccgcttgag gtagtggtcg aggaacaggg ggatcaccgc
     1021 gagctcgaac atcagcgcct cgatgatcgg cttgccggtg tcggcggcga tcttgcgggc
     1081 gtaggcgtcg ccgtgctcgg cgagcacgtg gcgtccgcgg ttgagcgcct cgaggcggac
     1141 ctcgagcggc gtgtcacgcc agcccgggaa cgccgtccgc gcgcgcgcca ccgcggcatc
     1201 gacggcgtcc tggccggcga tcgcatactc tcccaggcgc tccccggtga ccgggctgtg
     1261 cttctcgaac aagcggctgg gggacggggt ggacatcgtg gggtcggcga gggtcatgca
     1321 ggcgctcctt gggcgggccc agacactagc aggcccgtaa caaatttggg tccccgaccc
     1381 cacggacccc cttgcagcgg gccgacctgg taccatccgc ccgggtatga cgacgggttc
     1441 accggacgag ctttatcgcc gctacctcgg gatcctcggc tgggtcgggg accccggcct
     1501 gcacgagctg gtcagcagcc acctgatcaa ggtgccgtac gagaacgtgt cgacgctgct
     1561 gcgctcggcc gagccggccg gcgcgaccac cctgacgccg atcgcggagt tcctggagaa
     1621 catcgccgag cgcgacctcg gcggcaacgg ctattcgatc ccgatccact tcgcctcgct
     1681 gctgcagttc ctcgactacg aggtcgagct gctcggcgcc gacatcggcg gggctgcgcg
     1741 cgcgcacgcc gtgtgccgcg tgcgctccga ggacaccgac tacctcgtcg acatcggcca
     1801 cggcgccccg ttctaccagc cgatcccgct cgatacgctg ccggtgcacg tgccgcacgg
     1861 cgactatcaa tacgtcatcg accgccacgc ctccggcccc ggcgcgatcg aggtcacgac
     1921 cctgcgcacc ggcaccaggg tccagagcta cgtcgccaag ctcccggcgc tcggccccga
     1981 gggcttcgcc ccggcgatcg cccaggcgcg cgccaccgac gcccggaccc tgcgcacgct
     2041 ccggatctcg cggtttttcg cctcgcgcgt gctcgagatc ttcgacaacc acctcgtcac
     2101 cacccaccac aacatcacca cggtgacccc gatccgcgac atcgaggagc tcgagacgat
     2161 cgtccgcgag cagctccagc tggtgcgctg cccggtccgc gaggcggtcg agatcctgcg
     2221 ctcgcgcggc gtcgacatct tcgccaactc cgccccgccg accccctgag aggcagccgc
     2281 gtccatgccg agcctggcgc ggacgctgca gagtccctgg ttcatcctcc tgctggccgc
     2341 ctgcggccag ccaccggcac ctgcgggccc cgaggcgacc acaccggccc cggtgcgcga
     2401 ggtcgccgtc accttcgacg acctcgtgat cggcggccgg atcctcgagc tgccgcgcat
     2461 gcggacgatg actgcggacc tgctgcgcac gatcaccgcc gagggcgtcc ccgcggtcgg
     2521 cttcgtcaac gaggccaagc tgtgcgactg cgacgagcgc agcggccgga tcgaggtgct
     2581 gcgcatgtgg gcggccgccg gcctcgagct cgccaaccac acgtacagcc acccgtcgct
     2641 gacctgcacc ccgctcgccg actaccaggc cgacgtcctg cgtggcgagc ccgtcacgcg
     2701 cgccctgatg gccgagcacg gcaagccgtt tcgctggttt cgccacccgt acctgcacac
     2761 cggcccgacc ctcgaggtcc gcgccgcctt cgagccgtgg ctcgcggagc gcggttacac
     2821 cgtcgcgccg gtgaccctcg acaactccga ctggatgttc aacttcgtct acaccgacgc
     2881 gaagacccgc ggtgacgtgg cgctgatgcg cagggtgggg gaggccttta tcgcctacgt
     2941 cgacgaggtg atggcctttt atgagagcgc cgagcaggcc ctgttcgccc gcccgatccg
     3001 ccatgtcttg ctgttgcacg ccaacgagct caacgccgac tggttcgacg atctcgccgc
     3061 gctgctgcgc cgccgcggct accgcttcat cacgctcgag gccgcgctgc aagaccccgc
     3121 gtaccgcgag cccgaccgct acgccggccc cgccggggtg tcatggatgt accgctggga
     3181 cttcacgcgc gggcgcgagc agatcgactg gtccagcgag cccgagccgc cggcctttgt
     3241 ccgcgagctc tacgacctcg cccagcgcgg cccaccccgt gatcagaact cgatccccac
     3301 gccgaccgtc caggtgccgt gacgggtgcg tgcgaagctg ggagcgacgc gaaacgagct
     3361 gtggcggcgc tgccagtcgg tgaattcgcg ccgccgctgg accccgtagc cgagcatcgc
     3421 cgccccaccg atggcgccga ccagcccgaa gatcagcgcc gccgccccac tcgcggtttg
     3481 caggccgttg ccggtgaaca gcgcgtcctc ctcctcctcg gtggtcgtcg caccgcggcg
     3541 tagctgcacg accccggtcg ggatcagcac cgcgccagcc accagagcga tgatgccgcc
     3601 ggcgatcagc ccggccccgg tcggtggtgg gctgttgtca catcgcgtca cgggccgccc
     3661 ccgcgccccc gcgcctgtac ggtcccatgg cgacgcgcgg gcctcgagcg acaccgacgc
     3721 cagcgggagc acgagcaggg cagggagcaa tcgacgcgcg gaccgtctcg ccatttcttc
     3781 gagaaaagca cggcgagcgc cgccctcgca attggtcggg cgcccccact tgatgcacgg
     3841 cacagcgcgc ggtcgatcac tcgcaccggc ctcacatcga acgcgaggcc gcgggccccg
     3901 gcaccctagc ccgcgtcaac gacgacctcg atgcagcgcg ccgccgcgtt ggcccgtgac
     3961 cgggcgtcct cgacggtctc tccgtacgtc aatacgacgc ccatgcggcg atacttgcgg
     4021 gtgtccggct tggcgaacag ccgcacgcgc gcgcgggggt cctgcagcgc ccgcccgagc
     4081 cccaaaaacc gcgggttatg ccccgcgcgc tcggccagga tcaccgagga ggcccccggc
     4141 gcgcgctgaa tgacccgcac gatcggcagc ccgaggatcg cgcgcacgtg cagctcgaac
     4201 tcgctgaggt cctgcgtcgc catcgtcacc atgccggtgt cgtgcggacg cgggctgacc
     4261 tcgctgaaga tcacgtcgtc gccgacgagg aagaactcga cgccgaacag gccgaagccg
     4321 ccgagcgccc cggtcacctg ctccgcctgg gcctgggcct tttgcagcgc cgcttccgac
     4381 atcgcgtgcg gctgccacga ctccatgtaa tcgccgcgct cctgacggtg gccgatcggc
     4441 gggcagaaca gcgtgccctc ctcgcgcgtg cggatcgtca gcagcgtgat ctcgctgtcg
     4501 aagcggacaa agccctcgac gatcacgcgc gccgacttgc cgcgcgcgcc ggccagcgcg
     4561 tagtcccacg cggccggaag atctgcgcgg tcgcgcagcg tgctctggcc cttgcccgag
     4621 ctcgacatca ccggctttat cacgcacggc agcccgatct tcgccaccgc ctcctcgacc
     4681 tgctcgagcg tgtcggcgaa cgcgaagggg ctcgtcggca ggccgagggt ctcggcggcg
     4741 agccggcgga tgccctcgcg gtccatcgtc agccgcgtcg cgcgggcggt cgggatcacc
     4801 gtccagccct cggcctcgag ctcgacgagc gcgtcggtgg cgatcgcctc gacctccggc
     4861 acgatgaagt gcggcttctc gcgaccgacg accgcgcgca cggcgtcgcg gtcctgcatg
     4921 tcgaacacgt ggctgcggtg cgcgatctgc atcgccggcg cgtcgcggta gcggtcgcag
     4981 gcgatcacct cgatgccgag ccgcatcgcc tcgatggcaa cctcgcggcc gagctcaccg
     5041 gagccgagca gcaagatccg cgtggccgag ggggagaggg gggttccgag ttcgaggtcc
     5101 atgcgcgctc gcaggataga ggttcggacc cgcgcacggc aagccgcgac gcgccccctc
     5161 gcgccgcgcc acgacacgtg acacagcgca tgcgcgcccg accgaacgcc ccggcgcagc
     5221 tcacgcgcgt cggagcgcgg ctcacgccgt tcgggccgcg ccctcgacga tatgtcccga
     5281 acgacatgtc gcatcggaca tgttcacacg ggcagaattt gctgtcgctc gtcgccgcgc
     5341 ggcgtagcgc cggcgccc
//
LOCUS       DASWNJ010000443         2821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_323203, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000443 DASWNJ010000000
VERSION     DASWNJ010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323203"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VGB85_07625"
     CDS             complement(<1..548)
                     /locus_tag="VGB85_07625"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY0133932.1"
                     /translation="MPRTRWTIGNWKQNLLRGAAEDLATAVVGDLPEPVAEGGKQVRV
                     GIAPSYVALDVVRPWVRPQGPLFLFAQDVAAQEEGAFTGEVGPGMLQDAGVHGTLVGH
                     SERRLHFGETDESVARKVQVALEAELRVVLCVGEGLEVREAGEHENHVVSQLSEALRQ
                     LPPELVDERLIIAYEPVWAIGTG"
     gene            complement(551..1786)
                     /locus_tag="VGB85_07630"
     CDS             complement(551..1786)
                     /locus_tag="VGB85_07630"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY0133933.1"
                     /translation="MPSAPDSPLNLPLLDDLDVENRRVFVRVDFNVPLKGSEVRDDTR
                     IKATLPTLRRLRERGARLILASHLGRPDGAPDPKYSMEPVGARLAELLDCEVRLPDEV
                     VGDGVTKLVLDTRAQHIVLLENLRFHPGETKNDPAFAQQLAGLCEAYVNDAFGASHRA
                     HASVVGLPRLINARAAGLLLASETEALGRLVHRPEHPFVTLIGGAKVSDKLPVLLATL
                     ERLKAGDSILIGGAMANTFLAARGAELGASLQEPDRLSDCRSLLTRAEARDIRVLLPG
                     DLRIGAGRDAKEARVVRVSDGGIGDKDMALDIGPDTETKFAAAIKGAKMLFWNGPMGL
                     FENPAFAAGTLAVARAVAACKGFTVVGGGDSVAALQDSGVADQIDHISTGGGASLEML
                     EGTTLPGVEALMPATTRSN"
     gene            complement(1881..2075)
                     /locus_tag="VGB85_07635"
     CDS             complement(1881..2075)
                     /locus_tag="VGB85_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133934.1"
                     /translation="MQPTAEPRDTSRRAVSILARSLFRQMRDQGYAPEQIIALSSELI
                     ELVSTDLRQERAMDARWAAE"
     gene            complement(2297..2656)
                     /locus_tag="VGB85_07640"
     CDS             complement(2297..2656)
                     /locus_tag="VGB85_07640"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY0133935.1"
                     /translation="MTTRRASQDRFVCDLDVELDAGRVRVTGRARDLNEVAVCLTARE
                     PIEPGNKVQVHLRLVLEWGASEALVVPGLVAWLTPTEGMQQIGVVFNTLLPDVRQRLS
                     TLTKVLYGQIALPAPAN"
BASE COUNT          414 a          947 c         1040 g          420 t
ORIGIN      
        1 ccggtgccga tcgcccacac cggctcgtag gcgatgatca ggcgctcgtc gacgagctcg
       61 ggcggtagtt gacgaagagc ctcggagagc tgcgaaacca catggttttc gtgctcgccg
      121 gcctcacgga cttcgagccc ctcgccgacg cagagcacga cccgcagctc ggcctcgagg
      181 gcgacctgga ccttgcgcgc gacgctctcg tcggtctcgc cgaagtgcag gcggcgctcg
      241 gagtgaccta cgagcgtgcc gtgcacgccg gcgtcctgca gcatcccggg tccgacctcg
      301 ccggtgaacg cgccctcttc ctgggcggcg acgtcctggg cgaacaggaa cagggggccc
      361 tgcgggcgga cccaggggcg cacgacgtcg agggcgacgt agctcggggc gatgccgacg
      421 cggacctgct tgccgccctc ggcgaccggc tccggcaagt cgccgacgac ggccgtcgcc
      481 aggtcctcgg ccgcgccgcg cagcaggttc tgcttccagt tgccgatcgt ccagcgggta
      541 cgcggcacgg tcagttgctc ctcgtcgtcg cgggcatcag ggcctcgacg cccggcaggg
      601 tcgtgccctc gagcatctcc agggaggcgc cgccgccggt agagatgtga tcgatctggt
      661 cagcgacccc ggagtcctgg agcgcagcga cgctgtcgcc gccgccgacc acggtgaagc
      721 ctttgcaggc cgcgaccgcg cgcgcgaccg cgagcgtgcc ggcggcgaag gccgggttct
      781 cgaacagacc catcggcccg ttccagaaca gcatcttcgc gcccttgatc gccgccgcga
      841 acttggtctc ggtgtcgggg ccgatgtcga gggccatgtc cttgtcaccg atgccgccgt
      901 cggagacgcg gacgacccgc gcctccttcg cgtcccggcc ggcgccgatc cggagatcgc
      961 ccggcagcag cacacggatg tcacgcgcct cggcgcgggt cagcaggctg cggcagtcgg
     1021 acaggcgatc gggctcctgc aacgaggcgc cgagctcggc cccgcgggcg gccaggaagg
     1081 tgttggccat cgcgccgccg atgaggatgc tgtcgccggc cttgagccgc tcgagcgtgg
     1141 cgagcagcac cggcagcttg tccgagacct tggcgccgcc gatgagggtg acaaaggggt
     1201 gctcggggcg gtggaccagg cggccgagcg cctcggtctc gctggcgagc agcaggccgg
     1261 cggcccgggc gttgatcagg cgcggcaagc cgacgaccga ggcgtgggcg cgatgcgagg
     1321 cgccgaaggc gtcgttgacg taggcctcgc acagaccggc gagctgctgc gcgaacgcgg
     1381 ggtcgttctt ggtctcgccg gggtggaagc gcaggttctc gagcagcacg atgtgctggg
     1441 cgcgggtgtc gagcacgagc ttggtgacgc cgtcgccgac gacctcgtcg ggcaggcgga
     1501 cctcgcagtc gagcagctcg gcgaggcgcg ccccgaccgg ctccatcgag tacttcgggt
     1561 ccggcgcgcc gtcgggccga ccgaggtgcg aggcgaggat cagccgggcg ccgcgctcac
     1621 gcaggcggcg cagcgtcggc agcgtcgcct tgatgcgcgt gtcgtcgcgg acctcgctgc
     1681 ccttcagagg gacgttgaag tcgacccgga cgaacacgcg tcgattctcg acatcgaggt
     1741 cgtccagcag aggcaggttc agagggctgt cgggggccga cggcatgggc gcaagcaata
     1801 ccgaccgggg ccgggcaagt ccacgccggg ggtcgtgact tttcgtccga cccggccgcg
     1861 ggctcgtcgc gtacccggcg ttactcggcg gcccagcgcg cgtccatcgc ccgctcctga
     1921 cgaagatcgg tgctgacgag ctcgatcaac tcggaggaga gcgcgatgat ctgttcgggc
     1981 gcgtagccct gatctcgcat ctgccgaaac agcgagcgag cgaggatgct gactgcgcgt
     2041 cggctggtgt cccggggttc ggcggtgggc tgcatggtgg tttctcgggt ggctcctatg
     2101 caaaccacag gcgcggggca ctgtattcca ccggtttgag cttatgtcaa agattttcga
     2161 tatttagcgc gggattgccc ggcgcgtggc ggcgcatttg gacccagtct gtgcacggct
     2221 ctgcacgtac gggcacctac accgcgtgaa ggatcatgca cggttgcgca gtgccgcggg
     2281 agattgcggc gcggactcag ttcgccggcg cgggcaaggc gatctgaccg tagaggacct
     2341 tggtcagggt cgacagtcgc tggcggacgt cgggcagcag cgtgttgaag acgacgccga
     2401 tctgctgcat gccctcggtc ggggtcaacc acgcgaccag gccggggacc acgagcgctt
     2461 cgctggcgcc ccactcgagc acgagccgca ggtgcacctg gaccttgttg ccgggctcga
     2521 tcggctcgcg cgcggtcagg cacaccgcga cttcgttgag gtcacgcgcg cggccggtga
     2581 cgcggacgcg gcccgcgtcg agctcgacgt cgaggtcaca cacgaagcga tcctgcgaag
     2641 cacgcctggt tgtcacgctg gcaggatatc aaacctggac aggcggccac cagtgggcac
     2701 gcgcgcgcac tttcgcgtgc tcactgcgcg ggcgccggag tgacggcgcg acgcggcaag
     2761 gcgagcccgg tcatcgcggt caacacggcc tcgacctcgg cgagccgcat gtgcctgccg
     2821 a
//
LOCUS       DASWNJ010000444         1629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_360351, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000444 DASWNJ010000000
VERSION     DASWNJ010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360351"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1629
                     /locus_tag="VGB85_07645"
     CDS             <1..>1629
                     /locus_tag="VGB85_07645"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEY0133936.1"
                     /translation="VVGNLIILTDKGSSIRRLEQIVTELDTPTASEKIFFYQLQYAKA
                     EEVAEIVREIFGEGTKGEAAPAKGKRGAAAAPSAINRVIVDERSGTLIVITNEADYVV
                     IRRLIESLDVKLPGGGGRIHVRKLKNADAQAVATVLSALATGAKQAAQAGTDKNKAPG
                     GAISADLFSGDVKITPDIDTRSLVIIASAADYKNLEPVIDKLDMERRQLYIEIYMLEV
                     SIKHSTDVGAGAHFGTQLGDSSSNGIAVIGSAPTAALNSLILNPAAFKGLAGGIVGPL
                     VGGSGQLLGLGRDVPGFGAVIQALQQSSDVNVVSEPHMYAADNQEANIEIGRNVPTQG
                     ALSALGGANGIGGLPVQSIERQDVTLRIKVKPYINDERNVTMDVEVEDRDIADTSPTL
                     GVTTTKRRFKLDKIVGRDGQPFVLGGLIRDRESETVNQVPGLGSIPLLGWLFKSRQKA
                     KEKVNLLLIMVPHIIDSPDDVRRIHERRDKERLEFIERETNFKRRELPSNVNYRKKSG
                     ILASIDKEARRMEQEELNLRRAEDEMRREIITGVL"
BASE COUNT          306 a          609 c          503 g          211 t
ORIGIN      
        1 gtcgtcggca acctgatcat cttgaccgac aagggctcga gcatccgtcg cctcgagcag
       61 atcgtcaccg agctcgacac gccgaccgcc agcgagaaga tcttcttcta tcagctgcag
      121 tacgccaagg ccgaggaggt cgcggagatc gtccgtgaga tcttcggcga gggcaccaag
      181 ggcgaggcgg ccccggccaa gggcaagcgc ggcgccgcgg cggctccctc ggcgatcaac
      241 cgcgtgatcg tcgacgagcg ctccggcacc ctgatcgtga tcaccaacga ggccgactac
      301 gtcgtcattc gccgcttgat cgagagcctc gacgtcaagc tgcccggcgg cggcggccgg
      361 atccacgtcc gcaagctcaa gaacgccgac gcccaggcgg tcgccaccgt gctctccgcg
      421 ctggcgaccg gcgccaagca ggccgcgcag gccggcaccg acaagaacaa ggcccccggc
      481 ggcgccatct cggccgacct gttctccggc gacgtcaaga tcacgccgga catcgacacc
      541 cgctcgctcg tcatcatcgc ctcggcggcc gactacaaga acctcgagcc cgtgatcgac
      601 aagctcgaca tggagcgccg ccagctctac atcgagatct acatgctcga ggtgagcatc
      661 aagcacagca ccgacgtcgg cgccggcgcc cacttcggca cgcagctcgg cgacagctcg
      721 agcaacggca tcgccgtcat cggcagcgcc ccgaccgccg cgctcaactc gctgatcctc
      781 aaccccgccg cgttcaaggg cctcgccggc ggcatcgtcg gtccgctcgt cggcggctcc
      841 ggtcagctgc tcggcctcgg ccgcgacgtg cccggcttcg gcgccgtcat ccaggccctg
      901 cagcagagct cggacgtcaa cgtcgtctcc gagccgcaca tgtacgcggc cgacaaccag
      961 gaggccaaca tcgagatcgg ccgcaacgtg ccgacccagg gcgcgctgtc ggccctcggc
     1021 ggcgccaacg gcatcggcgg cctcccggtc cagtcgatcg agcgccagga tgtcaccctg
     1081 cgcatcaagg tcaagccgta catcaacgac gagcgcaacg tgacgatgga cgtcgaggtc
     1141 gaggaccgcg acatcgccga caccagcccg acgctcggcg tcacgaccac caagcgccgc
     1201 ttcaagctcg acaagatcgt cggccgcgac ggccagccct tcgtgctcgg cggcctgatc
     1261 cgcgaccgcg agagcgagac cgtcaaccag gtccccgggc tcggctcgat cccgctgctc
     1321 ggctggctgt tcaagagccg ccagaaggcc aaggagaagg tcaacctgct cctgatcatg
     1381 gtcccgcaca tcatcgactc gcccgacgac gtgcgccgca tccacgagcg gcgcgacaag
     1441 gagcgcctcg agttcatcga gcgcgagacc aacttcaagc gccgcgagct gccgtccaac
     1501 gtcaactacc gcaagaagtc cggcatcctc gcctcgatcg acaaggaggc ccggcgcatg
     1561 gagcaggagg agctcaacct gcgccgcgcc gaggacgaga tgcggcgcga gatcatcacc
     1621 ggcgtgctc
//
LOCUS       DASWNJ010000445         1936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_256110, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000445 DASWNJ010000000
VERSION     DASWNJ010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256110"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1431)
                     /locus_tag="VGB85_07650"
     CDS             complement(<1..1431)
                     /locus_tag="VGB85_07650"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY0133937.1"
                     /translation="MTATTSSIRPELATAIADPGFTPGRADLQGVIDLLADADEDAAE
                     RAERALLRAGSAAGPALQARLSASVAPLRARIVRVLGRLAIADAGLRAPLLACIADTD
                     PRARRNAIVALGKLADPAAAAALVAHARVETSTPHLRSLVEALGKCGDEQALAWLDGL
                     DDPDPELQRLRGRARIMLGRSLGRETSDAAIDDDAVAAAPRSVIATCRPGLEDILVST
                     IDPALSPGVAGPGKVGLKSAGPLRELGRARTLMTLGFPLPRVRLAPGPDALVEALTAA
                     LTGPEAREIFATWSKGQVRYRLAWARGGHRRAVVWKVAAAVGKVRPDFINDPTASPWE
                     VVVDDGGGQLQVELRPRWTDTRFAYRVGDVPAASHPTIAAALAMLGGVRADDVVWDPF
                     MGSGLELVERGLLGPYARLVGTDLDSKALATATANLKAAGLERFEVVEADATTYVPAD
                     VSLIITNPPMGRRVQRGDVAPLLTGFV"
     gene            complement(1463..>1936)
                     /locus_tag="VGB85_07655"
     CDS             complement(1463..>1936)
                     /locus_tag="VGB85_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133938.1"
                     /translation="PNTTVKCLSGPCPVTTAELITAPYPPLAATAQGTVIELEFHAAD
                     HRPHVGKYTIFPGTNQSPFSLERIVVLPPDSATITFQGAPEGATVQCVSGPCPDKKQY
                     ALESFPAVKLAKDDQTLLLRFNAPGYRTAMSSFQVSRGPNVIPVLMEKGTNGRNR"
BASE COUNT          252 a          711 c          705 g          268 t
ORIGIN      
        1 gacaaagccg gtcagcagcg gcgcgacgtc gccgcgctgg acccgtcggc ccatcggcgg
       61 gttggtgatg atcaggctca cgtcggcggg cacgtacgtg gtcgcgtcgg cctcgaccac
      121 ctcgaagcgc tcgaggccgg cggccttcaa gtttgcggtc gcggtcgcca gcgccttgct
      181 gtcgaggtcc gtgccgacga gccgcgcgta gggcccgagc agcccgcgct cgaccagctc
      241 gagcccggag cccatgaagg ggtcccacac gacatcgtcg gcccgcacgc ccccgagcat
      301 cgccagcgcc gccgcgatcg tcgggtgcga ggcggccggg acgtcgccga cgcggtacgc
      361 gaagcgcgtg tcggtccagc gcgggcgcag ctcgacctgc aactgccccc caccgtcgtc
      421 gaccacgacc tcccacgggc tcgccgtcgg gtcattgata aagtccggcc ggaccttgcc
      481 gaccgccgcg gcgaccttcc acaccaccgc cctgcggtgc cctccgcggg cccacgcgag
      541 gcgatagcgg acctgtccct tcgaccatgt cgcgaagatc tcgcgcgcct cggggccggt
      601 cagtgcggcc gtcagcgcct cgaccagcgc gtcgggtccg ggcgccaggc gcacgcgcgg
      661 gagcgggaag ccgagcgtca tcagcgtgcg cgcgcggccg agctcccgca gcggccctgc
      721 actttttaaa ccgaccttgc cgggcccggc gaccccgggc gacagcgccg gatcgatcgt
      781 gcttacaaga atgtcctcga ggccaggtcg acacgttgcg atcaccgacc gcggcgccgc
      841 ggcgaccgcg tcgtcgtcga tcgcggcgtc gctggtctcg cgcccgaggc tgcgcccgag
      901 catgatgcgc gcccgcccgc gcagtcgctg aagctccggg tccgggtcgt ccaggccgtc
      961 gagccacgcg agcgcctgct cgtcgccaca cttgccgagc gcctcgacga gcgagcgcag
     1021 gtgcggcgtg ctggtctcga cgcgcgcgtg ggcgaccagc gccgccgcgg ctgccgggtc
     1081 ggcgagcttc ccgagcgcga cgatcgcgtt gcggcgcgcg cgggggtccg tgtcggcgat
     1141 gcaggcgagc agcggcgcgc gcagcccggc gtccgcgatc gccagccgac cgagcacacg
     1201 cacgatgcgg gcccgcagcg gggcgacgga cgcggacagc cgggcctgca acgccggccc
     1261 cgcggccgag ccggcgcgca gcagggcccg ctcggcccgc tcggccgcgt cctcgtcggc
     1321 gtcggcgagc aggtcgatca cgccctggag gtcggcgcgt ccgggcgtga agcccgggtc
     1381 ggcgatcgct gtcgcgagct ccggtcgtat gctcgaggtc gtcgcggtca cgcggcgtgc
     1441 accatcgtca tcttcaaaac gatcatcggt tcctgccgtt ggtgcccttc tccatcagca
     1501 ccgggatcac gttgggcccg cggctgacct ggaacgagct catcgccgtg cggtagcccg
     1561 gcgcgttgaa gcgcagcagc agggtctggt cgtccttggc gagcttgacc gccgggaagc
     1621 tctcgagcgc atactgcttc ttgtccgggc acggcccgct gacacactgg accgtcgcgc
     1681 cctcgggcgc gccctggaac gtgatcgtcg cgctgtcggg cggcagcacg acgatgcgct
     1741 cgagcgagaa cgggctctgg ttcgtgcccg ggaagatcgt gtacttgccg acgtgcgggc
     1801 ggtgatcggc ggcgtggaac tcgagctcga tcaccgtgcc ctgagcggtc gccgcgagcg
     1861 gcgggtacgg agccgtgatc agctccgcgg tggtcaccgg gcacggaccg ctgagacact
     1921 tgaccgtcgt gttcgg
//
LOCUS       DASWNJ010000446         3722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_256133, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000446 DASWNJ010000000
VERSION     DASWNJ010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256133"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1311)
                     /locus_tag="VGB85_07660"
     CDS             complement(154..1311)
                     /locus_tag="VGB85_07660"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0133939.1"
                     /translation="MSWSRTLIVLLAASAWPLSCCCEEPECGDDTRDPGELCFLEERV
                     EVDLELDMPLALRGGLYDDDELGDLLVIGTDETGVTGRLLLGDGDGLAKPGKVAVSGC
                     SAYPIAGDLDGDGRHDLVFATCTNGLVVYRATDQGFTAGLEIPVGVAVRQGAIADVDG
                     DGLRDILVLGVDGAGVPALSFAQALTDGTFAPAYLTPQPVPALESFEPNAMATGRVQR
                     DGRFEIVFAEAERPGGLARTHYVGKGTFAPPEPLTLALRPAGLALRDLDNDDRLDLIT
                     ADRAREELAVVLDGGDPDGIRTPLEPLWQSFSLGQMDDDGWLDLVLIREERVEIRRGT
                     GDGSFNEGIEVLFTAPVIELALIDLNGDGRDDLIAGTFAGDAPLTITLSGP"
     gene            1470..2060
                     /locus_tag="VGB85_07665"
     CDS             1470..2060
                     /locus_tag="VGB85_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133940.1"
                     /translation="MRLSSALVLQLLVSIAACGPEKGDTATDSGASSGSTMTEPTGAT
                     AQPTTTGDTTSDDVAEQFAAGCGFPRPCADASIDCGSAFEPAVCTQDYSPQIKCALEK
                     LAAGEGFALKVDVSNYNAENEQYHIVFDGKGGAARQHWAGLPFGSPQKCDLLPKEKFA
                     ECLAVANDDPLHSECMVVFNWMPNCVGGDDVVCPAP"
     gene            complement(2187..3473)
                     /locus_tag="VGB85_07670"
     CDS             complement(2187..3473)
                     /locus_tag="VGB85_07670"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEY0133941.1"
                     /translation="MADTLTITDNRTGKTYEVPIQDGTIRADALRQIKTGAEDFGLMT
                     YDPAFMNTASCRSSITFIDGDAGILRYRGYPIEQLAESSTYLEVAYLLLNGELPNSTQ
                     LATWTDTITKHTYVHEFLREGMKGFRPDAHAMGKLVSMLGTLSTFYPESKNVRDPKVR
                     AEHIVKIIAKMPTIAAWAHRHSVGRPYVYPDNELSYCGNFLNMLFRMAEPRFKPHPVL
                     ERALDVLFILHADHEQNASTSATRAVGSAETDPYCSLAAGAAALYGPLHGGANEAVLR
                     MLHQIADIKHVPDFIKSVKDGKGRLMGFGHRVYKNYDPRAKVIKKLAHDVFAVTGKNP
                     LLEIAIELETIALNDEYFIKRKLYPNVDFYSGLIYQAMGFPVEMFPVLFAIPRASGWL
                     AQWQEMLGDSEQKIARPRQIYTGIEIRDYTPIADRK"
BASE COUNT          567 a         1269 c         1301 g          585 t
ORIGIN      
        1 gtcggcatcg agctgcgcgc gccggaccgc atcgaggcgg tgttcgccga ggcgcgcgcg
       61 gccgcgaagg ccggcaaccc ggtgctgatc aacgcccacc tgcggcgcac cgacttccgc
      121 aaggggtcga tctcgatgtg aggccccggg cgctcagggc ccggacagcg tgatcgtcag
      181 cggcgcatcg ccggcgaagg tgccggcgat caggtcgtcg cggccgtcgc cgttgaggtc
      241 gatcagcgcc agctcgatca ccggcgccgt gaacagcacc tcgatcccct cattgaagct
      301 gccgtcgccg gtcccacgcc ggatctcgac ccgctcctcg cggatcagca cgaggtcgag
      361 ccagccgtcg tcgtccatct ggccgagcga gaagctctgc cacagcggct ccagcggcgt
      421 gcggatgccg tccgggtcgc cgccgtcgag caccaccgcc agctcctcgc gcgcccggtc
      481 ggcggtgatc aggtcgaggc ggtcgtcgtt gtcgaggtcg cgcagggcca ggccggcggg
      541 gcgcagggcg agcgtcagcg gctcgggcgg cgcgaacgtc cccttgccga cgtaatgcgt
      601 gcgtgcgagc ccgccgggcc gctccgcctc cgcgaacacg atctcgaagc gaccgtcgcg
      661 ttgcacccgg ccggtcgcca tcgcgttggg ctcgaagctc tcgagggcag ggaccggctg
      721 cggcgtcagg taagccggcg cgaacgtgcc gtcggtcagc gcctgcgcga agctcagcgc
      781 cggtacgccc gcgccgtcga ccccgagcac caggatgtca cgcaggccgt ccccgtcgac
      841 atccgcgatc gccccctgcc gcaccgccac cccgaccggg atctcgagcc ccgccgtgaa
      901 cccctggtcg gtcgcgcggt acacgaccag cccgttcgta catgtcgcga agaccaggtc
      961 atggcggccg tcgccgtcga ggtcgcccgc gatcgggtac gcgctgcacc ccgacaccgc
     1021 cacctttccc ggcttcgcca gcccgtcgcc gtcgccgagc agcagccgcc cggtcacgcc
     1081 ggtctcgtcg gtgccgatca ccagcaggtc gccgagctcg tcgtcgtcgt agagcccgcc
     1141 gcgcagcgcg agcggcatgt cgagctccag gtcgacctcg acccgctctt ccaggaagca
     1201 cagctccccg ggatcgcggg tatcgtcgcc gcactcgggc tcctcgcagc agcacgacag
     1261 cggccacgcc gacgcggcga gcagtacgat cagcgtccgc gaccacgaca tctcgccagc
     1321 gtatcgcctc cgcgcgcgtc atgtcccggg gcaccgactc caagatgaga catcgatcac
     1381 gcttgcgcgg atcggggggc ggacgaccgc gcgtcacatc gctttgcact gcctgcggag
     1441 cagtgttcca cgggtctatc ctcgccggca tgcgactgtc atcggccctc gttctccagc
     1501 tcctcgtatc gatcgccgcg tgtggcccgg agaagggtga cacggccacg gacagcggcg
     1561 cgagcagcgg gtcgacgatg accgagccga ccggagcgac cgcccagccc accaccaccg
     1621 gcgacaccac cagcgatgac gtggccgagc agttcgccgc cggttgtggc ttcccgcggc
     1681 cctgcgcgga cgcctcgatc gactgcggct cggccttcga gcccgcggtg tgcacccagg
     1741 actactcgcc gcagatcaag tgcgcgctcg agaagctggc cgccggcgag ggcttcgcgc
     1801 tcaaggtcga cgtctcgaac tacaacgccg aaaacgagca gtaccacatc gtcttcgacg
     1861 gcaagggcgg cgccgcgcgc cagcactggg cgggattacc ctttggctcg ccccagaagt
     1921 gcgacctgct gccaaaggag aagttcgccg aatgcctcgc cgtggccaac gacgacccgc
     1981 tgcactcgga gtgcatggtc gtcttcaact ggatgcccaa ctgcgtcggc ggcgacgacg
     2041 tggtgtgccc ggctccctga cgacgccgga cgcggccagg cgtgctcgcc cgcggctcgc
     2101 catcgaagcc ccgagccgcc cggatcaccg aacgaacgaa gccgccgatc ccttcggatc
     2161 gacggcctcg tcgtcatcgc ggtcgcttac ttgcggtcgg cgatcggcgt gtagtcgcgg
     2221 atctcgatgc cggtgtagat ctggcgcggg cgggcgatct tctgctcgct gtcgccgagc
     2281 atctcctgcc actgggccag ccagcccgac gcgcggggga tggcgaacag cacggggaac
     2341 atctcgaccg ggaagcccat cgcctggtag atgaggcccg agtagaagtc gacgttcggg
     2401 tacagcttgc gcttgataaa atactcgtcg ttgagggcga tcgtctcgag ctcgatcgcg
     2461 atctcgagca gcgggttctt gccggtgacc gcgaagacgt cgtgggcgag cttcttgatc
     2521 accttggcgc gcgggtcgta gttcttgtag acgcggtggc caaagcccat caggcggccc
     2581 ttgccgtcct tgacgctctt gatgaagtcg gggacgtgct tgatgtccgc gatctggtgg
     2641 agcatgcgca gcacggcctc gttggcgccg ccgtgcagcg ggccatacag agcggccgcg
     2701 ccggcggcga ggctgcagta cgggtcggtc tcggcggatc cgacggcccg cgtggcgctc
     2761 gtcgaggcgt tctgctcgtg gtcggcgtgg aggatgaaca gcacgtcgag ggcgcgctcg
     2821 agcaccggat gcggcttgaa ccgcggctcg gccatgcgaa acagcatgtt gaggaagttg
     2881 ccgcagtacg acagctcgtt gtccgggtag acgtacgggc ggccgacgct gtggcgatgg
     2941 gcccacgcgg cgatcgtcgg catcttggcg atgatcttga cgatgtgctc ggcgcggacc
     3001 ttcgggtcgc ggacgttctt ggactccggg tagaaggtgc tcagggtgcc gagcatcgac
     3061 accagcttgc ccatcgcgtg cgcgtccggg cgaaagccct tcatgccctc gcgcaggaac
     3121 tcgtgcacgt aggtgtgctt ggtgatcgtg tcggtccagg tggcgagctg ggtcgagttc
     3181 ggcagctcac cgttgaggag caggtacgcg acctcgaggt aggtcgagct ctccgcgagc
     3241 tgctcgatcg ggtagccgcg gtagcgcagg atgcccgcgt cgccgtcgat gaaggtgatc
     3301 gacgaccgac acgaggcggt gttcatgaac gccgggtcgt aggtcatcag gccgaagtcc
     3361 tcggcgccgg tcttgatctg gcgcagcgcg tcggcgcgga tggtcccgtc ctggatgggg
     3421 acctcgtagg tcttgccggt tcggttgtcg gtgatcgtca gcgtgtcggc catgagaggt
     3481 cctgaggtcc tgaggtcctg aggtcctgga aggaccttgg ggagcggttt gtcagcgagg
     3541 gagtcagggc gcggagagcg acggtacaaa gacctgccag tgatcgacag ggcgctcgtc
     3601 cgcggtccaa ccgccgacca ggaaggtagc accggtcgac agtaaaacgg aatggccacc
     3661 gtgcacgcgc gtgctcgggg cgggcgacga ctcgaccgcc agcgtcgcgc ggtcgatgcg
     3721 gt
//
LOCUS       DASWNJ010000447         3426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_538917, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000447 DASWNJ010000000
VERSION     DASWNJ010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538917"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VGB85_07675"
     CDS             <1..652
                     /locus_tag="VGB85_07675"
                     /inference="COORDINATES: protein motif:HMM:NF024906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133942.1"
                     /translation="GANKTLRHLYLGTNGITERSAPRIAEFLARDCVLESFYLSCNRL
                     GDEGAAQIASGLAANRTISRVSLASNCIGPTGAAALAEALTGHPSIALFDLGFTKATV
                     AVGELGNFIGDEGARSLARMLEHNTSLWSLDLLHNYISQVGVNHLREALKLNRTLVHL
                     QLTQFGRVHNEPGKEELRAALQRNRALVPPELADRVEKIGLPDHITEIYSVYRTHS"
     gene            complement(811..2154)
                     /locus_tag="VGB85_07680"
     CDS             complement(811..2154)
                     /locus_tag="VGB85_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0133943.1"
                     /translation="MQLPPGPRAPSIVQTVQFLGRPTEFLTRCRARFGPIFTIRLAGM
                     PAMVCVGDAEGIAAIYRAPRGRFSVGSEDNALLPIVGRDSLLVLDGERHHRARKLLTP
                     PFTGERMRAYGDIIAAATRRAAAHWRAGAEVVAHDATQTLTQEVILRAVFGIADDRLD
                     AAMTVVREAVRTFASPVVLFEGLRRRLGLGSSPTLRRQRAALDRVSDSEIARHRGGEA
                     TDGEDILSRMLQARDESGAAMTDAELRDELCTTLFAGQKSTAIALSWALHWVLRTPGV
                     LASVRAELDAAGDIGADASSRLPYLSAVCDETLRVLPVFPVVAWILDEPMEILGVPLA
                     PGTRVAPGIYLVHRDARAYPEPEVFRPERFLQARRPGPFEYIPFGGGPRRCLGAAFAL
                     YEMKIALATLLRSFELELVDPRTRIALQGLMLAPADGVRLRVRATRGSPQSSPVL"
     gene            2197..>3426
                     /locus_tag="VGB85_07685"
     CDS             2197..>3426
                     /locus_tag="VGB85_07685"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0133944.1"
                     /translation="MQVASHRRMSDTPIEQFIVHHAIPEAAADALRELLATLAMSKTT
                     HPRGDEPTLQRSLGALTSPGNEDSSSMLAFSDDALRFIDLSLLGVGGMGEVWRVRDRA
                     LGRTVARKVLLGDLGHDQRLRVRFMQEAQIQAQLQHPGVVPLHDFGVLADGRPYFTMK
                     EIQGRTLGEVIRELHHVWRTQAPGAVSEGGWRATPSGWTLRRLLSAFHRVCETIAFAH
                     SRGVLHRDLKPSNMMVGEFGEVLVLDWGLTRVDDDAEPGVPLLDVERRDQTQLGAIGG
                     TLSYMPPEQALGDPSLLSKRSDVYSLGAVLYEILSDRVPYSDCAVMALLVQPPTPLRE
                     LLRREPDHPVVGDGLLRICETAMAREPADRYADASLMAAELAAWLDGAEKRVRAGELV
                     EEARAMQAKADEMRARAD"
BASE COUNT          521 a         1253 c         1194 g          458 t
ORIGIN      
        1 gggcgccaac aagacgctgc gccacctgta tctcgggacc aacggcatca ccgagcggtc
       61 cgccccgcgg atcgccgaat tcctggcccg cgactgcgtg ctcgagtcgt tctatctgtc
      121 gtgcaaccgc ctcggagacg agggggccgc gcagatcgcc agcggcctgg cggccaaccg
      181 gacgatctcc cgcgtgtcgc tcgcctccaa ctgcatcggc cccaccggcg ccgccgcgct
      241 ggccgaggcg ctgaccgggc acccgtcgat cgcgctgttc gacctcggct tcaccaaggc
      301 gaccgtcgcc gtcggcgagc tcggtaactt catcggcgac gagggcgcgc gctcgctcgc
      361 gcgaatgctc gaacacaaca cgagcctgtg gtccctcgac ctgctgcaca actatatctc
      421 gcaggtcggc gtcaaccatc tccgcgaggc gctcaagctc aatcgcaccc tggtgcacct
      481 gcaattgacg caattcgggc gcgtccacaa cgaacccggc aaggaggagc tgcgcgcggc
      541 gctgcagcgc aaccgcgcgc tggtcccgcc cgagctcgcc gacagggtcg agaagatcgg
      601 gctgcccgac cacatcaccg agatctacag cgtgtatcgc acgcactcgt gacggcaata
      661 cgacgagcac cgcaggcccg ctcggcgggc gcgcggtgca tgcgtcaaga tcacgggccg
      721 ctcaggtcac cggcaatacg gcgatctcga agcccagggt gtggtcgctg ccgaggtcgc
      781 gcgcacttcg ctggtcgacg ccggcctggc ctaaaggaca ggagatgatt gcggggaccc
      841 gcgggtcgcg cggacgcgca ggcgcacgcc gtcggcgggc gccagcatca gtccctgcag
      901 ggcgatgcgg gtccgggggt cgacgagctc gagctcgaag ctccgcaaca acgtggcgag
      961 ggcgatcttc atctcgtaca gcgcgaacgc ggcgccgagg cagcggcgcg ggccgccgcc
     1021 gaaggggatg tattcgaagg gccccgggcg ccgggcctgg aggaagcgct cgggtcgaaa
     1081 cacctccggt tcggggtagg cgcgcgcgtc tcggtgcacg aggtagatgc ctggtgcgac
     1141 ccgagtaccc ggcgcgagcg ggaccccgag gatctccatc ggctcgtcga ggatccacgc
     1201 gacgaccggg aacaccggca acacgcgcag ggtctcgtcg cagaccgccg acaggtacgg
     1261 cagccgcgac gacgcgtcgg ccccgatgtc gcccgccgcg tcgagttcgg cgcgcaccga
     1321 cgcgagcacc ccgggcgtgc gcaggaccca gtgcagcgcc cagctcaggg cgatcgccgt
     1381 cgacttttgc ccggcgaaca gcgtcgtgca cagctcgtcg cgcagctcgg cgtccgtcat
     1441 cgccgcgccc gactcgtcgc gggcctgcaa catccggctc aggatgtcct cgccgtccgt
     1501 cgcctcgccg ccgcggtggc gtgcgatctc cgagtcgctg acccggtcga gcgctgcgcg
     1561 ctggcgtcgc aaggtcgggc tggagcccag gccgagacgt cgacgcagcc cctcgaacag
     1621 gaccaccgga ctggcaaagg tacgcaccgc ctcgcgcacc accgtcatcg cggcgtcgag
     1681 gcggtcgtcg gcgatgccga acacggcgcg caggatcacc tcctgcgtca gcgtctgcgt
     1741 cgcgtcgtgg gcgacgacct cggccccggc gcgccagtgg gccgccgccc gccgcgtcgc
     1801 ggccgcgatg atgtctccat aggcccgcat gcgctccccc gtgaacggcg gcgtcagcag
     1861 cttgcgcgcg cgatggtggc gttcgccgtc gagcaccagc agcgagtcgc gaccgacgat
     1921 cggcagcagc gcgttgtcct cgctgccgac ggagaaccga ccccgcggcg cgcggtagat
     1981 cgccgcgatg ccctcggcgt caccgacgca gaccatcgcc ggcatcccgg cgagcctgat
     2041 cgtgaagatc gggccgaagc gggcccgaca ccgcgtcagg aactcggtcg ggcgtccaag
     2101 aaactggaca gtctggacga tcgaaggggc gcgggggccg ggcggaagct gcatgggaag
     2161 gcgtccgctc gacaataagc gggtttgtcc ggcaagatgc aagtggcttc ccaccgtcgc
     2221 atgtcggaca ccccgatcga gcagttcatc gtccaccacg cgatccccga ggcggccgcg
     2281 gacgcgctac gcgagctgct cgctacgctc gccatgagca agacgacgca cccgcggggc
     2341 gacgagccga cgctgcagcg ctcgctcggc gccctcacct cgccgggaaa cgaggacagc
     2401 agcagcatgc tcgcgttctc ggacgacgcg cttcgcttca tcgacctcag cctgctcggc
     2461 gtcgggggca tgggcgaggt ctggcgtgtg cgcgaccgcg cgctggggcg cacggtggcg
     2521 aggaaggtgc tgctcggcga cctcgggcac gaccagcggc tgcgcgtgcg cttcatgcag
     2581 gaggcgcaga tccaggccca gctgcagcac cccggcgtcg tccccttgca cgacttcggc
     2641 gtgctcgccg acggccgccc ctacttcacg atgaaggaga tccagggccg cacgctcggc
     2701 gaggtcattc gcgagctgca tcacgtctgg cgcacccagg cgcccggcgc ggtgtcggag
     2761 gggggctggc gcgcgacccc ctcgggctgg accctgcggc gcctcctcag cgcgtttcac
     2821 cgcgtctgcg agacgatcgc cttcgcccac tcgcgcggcg tgcttcaccg cgacctcaag
     2881 ccgagcaaca tgatggtcgg cgagttcggc gaggtcctcg tgctcgactg gggtctgacc
     2941 cgcgtcgacg acgacgccga gcccggcgtg ccgctcctcg acgtcgagcg ccgcgaccag
     3001 acccagctcg gcgcgatcgg tggcacgctc tcgtacatgc cgccggagca ggcgctcggc
     3061 gacccgtcgc tgctgtcgaa gcgctccgat gtctactcgc tcggcgccgt gctctacgag
     3121 atcctctcgg accgcgtgcc ttactccgac tgcgcggtga tggctctgct cgtccagccg
     3181 ccgacgccgc tgcgcgagct gctgcgccgc gagcccgacc acccggtcgt cggcgacggg
     3241 ctgctgcgga tctgcgagac ggcgatggcc cgcgagcccg ccgaccgcta cgccgacgcc
     3301 agcctgatgg ccgccgagct cgccgcctgg ctcgacggcg ccgagaagcg cgtgcgagcc
     3361 ggcgagctcg tcgaggaggc cagggcgatg caagcgaagg ccgacgagat gcgcgcgcgc
     3421 gccgac
//
LOCUS       DASWNJ010000448         1705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_538927, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000448 DASWNJ010000000
VERSION     DASWNJ010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538927"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VGB85_07690"
     CDS             <1..416
                     /locus_tag="VGB85_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0133945.1"
                     /translation="ADKHGKRVHGFTEAALHRMGNYGWPGNVREFENAVERAVVLCRG
                     ELIDELDLPPELRDPQAGHGRQITFSVGTPLEEVERRMISETLKFTRGDKRLAADLLG
                     IATRTIYRKLETGTGAHPEPAATPTTAVVDDEILH"
     gene            426..1481
                     /gene="gspC"
                     /locus_tag="VGB85_07695"
     CDS             426..1481
                     /gene="gspC"
                     /locus_tag="VGB85_07695"
                     /inference="COORDINATES: protein motif:HMM:NF041515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HEY0133946.1"
                     /translation="MEALFKQYFWVIKGLGLGITAALAASVITTYVGTSYIYEDEAAD
                     TDTDGGTGDTGGEEEDDDDKPSLVSSLGGSGSSSRSSTGDRTKMISTILSRNLFCPTC
                     AKVEVVPGATPTPAIDPVTGKPEDISGGPQPGEVPSSLPMRLVATMESSDPKFSWATL
                     RDEETGGAGPYWPGDKIYAGAVLVSVERRMVHVRHGSQLEYIELGVEPPKGAAKPVAS
                     AAKKTEDEPKPSGNMIEGARDAIKCETENACTIDRAWLEGVLSNPASLTKQARMMPSV
                     KDGETRGFKFYGIRPDSLPKLLNIKNGDLITAVNGTELKSMDGAMSLYAKLRRASNLQ
                     ITIERKGETITKELTIQ"
     gene            1478..>1705
                     /locus_tag="VGB85_07700"
     CDS             1478..>1705
                     /locus_tag="VGB85_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133947.1"
                     /translation="MTTRQNIRTSARNRVLAGLTTFLPALSLTLAGAPVRAQAPDAGS
                     TTTPARSPTGGGELVGDEGGGPAAGVPGTLPH"
BASE COUNT          303 a          658 c          535 g          209 t
ORIGIN      
        1 acgccgacaa gcacggcaag cgcgtccacg gcttcaccga ggccgccctg caccggatgg
       61 gcaactacgg ctggcccgga aacgtgcgtg agttcgagaa cgccgtcgag cgcgccgtcg
      121 tgttgtgccg cggcgagctc atcgacgagc tcgaccttcc gcccgagctg cgcgaccccc
      181 aggccggcca tgggcgtcag atcacattct cggtcggcac cccgctggag gaagtcgagc
      241 gccggatgat ctccgagacg ctaaagttca cccgcggcga caagcgcctg gccgccgacc
      301 tcctcggcat cgccacgcgc acgatctacc gcaagctcga gaccggcacc ggcgcgcatc
      361 cggagccggc ggcgacgccg accaccgccg tggtcgacga cgagatcctg cactgatcca
      421 cgacgatgga agccctcttc aaacaatact tctgggtcat caaggggctg ggcctcggga
      481 tcaccgcggc cctcgccgcc tcggtgatca cgacctacgt cggcacctcg tacatctacg
      541 aggacgaggc cgccgacacc gacaccgacg gtggcaccgg cgacaccggc ggcgaggagg
      601 aggacgacga cgacaagccc tcgctcgtgt cctcgctcgg cggctccggt agctcgtcgc
      661 gctcgtcgac cggcgaccgc acgaagatga tctcgacgat cctctcgcgg aacctgttct
      721 gcccgacctg cgccaaggtc gaggtcgtcc cgggcgccac gccgaccccc gcgatcgacc
      781 ccgtcaccgg caagcccgag gacatcagcg gcggcccgca gccgggcgag gtcccgagct
      841 cgctgccgat gcggttggtg gcgacgatgg agtcgagcga cccgaagttc tcctgggcga
      901 ccctgcgcga cgaggagacc ggcggcgccg gcccgtactg gcccggcgac aagatctatg
      961 ccggcgccgt gctcgtctcg gtcgagcggc gcatggtcca cgtgcgtcac ggcagccagc
     1021 tcgagtacat cgagctcggc gtcgagccgc ccaagggcgc cgccaagccg gtcgcctcgg
     1081 ccgccaagaa gaccgaagac gagcccaagc cgagcggcaa catgatcgag ggcgcccgcg
     1141 acgcgatcaa gtgcgagacc gagaacgcct gcacgatcga ccgcgcctgg ctcgagggtg
     1201 tgctgagcaa ccccgcctcg ctgaccaagc aggcccgcat gatgccctcc gtcaaggacg
     1261 gcgagacccg cggcttcaag ttctacggca tccgccccga cagcctgccc aagctcctca
     1321 acatcaagaa cggcgacctc atcaccgccg tcaacggcac cgagctcaag tcgatggacg
     1381 gcgccatgtc cctgtacgcc aagctgcgcc gggcctcgaa cctgcagatc accatcgagc
     1441 gcaagggcga gacgatcacc aaggaactca ccatccaatg accacgcgtc agaacatccg
     1501 cacaagcgct cgcaaccgcg tcctcgccgg cctcaccacc tttttgcccg cgctctcgct
     1561 gaccctcgcc ggcgcccccg tccgcgccca ggcgcccgac gccggctcga ccaccacgcc
     1621 cgcccgcagc ccgaccggcg gcggcgagct cgtcggcgac gagggcggcg gtccggctgc
     1681 cggcgtgccc ggcaccctcc cgcac
//
LOCUS       DASWNJ010000449         3886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_43589, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000449 DASWNJ010000000
VERSION     DASWNJ010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43589"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3886)
                     /locus_tag="VGB85_07705"
     CDS             complement(<1..>3886)
                     /locus_tag="VGB85_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEY0133948.1"
                     /translation="EAPFDLVILNSVVQYFPDAEYLTRTLLLAARAVRPGGAIFLGDI
                     RDHGLLGPFHASVQRHRLGDVDPAQLRARVHEAMRAEEELLVAPELFHALARRLGGHA
                     SVALKRGAGDVEMLRYRHDVTLHVGCAVEAVEAVTLAWSALPGGLDGLATRLAAGDDA
                     LIVAGIPNARVAADVALWDRLERGQAVTNGPAIDPEALHSLGERHGRRVTVRRSDAGL
                     GLVDALFEPMTAAPGRWPAAEATEASTNHPLRAAQERALVQQLRAALQRRLPEYAIPA
                     RFMVCDALPLTAHGKVDRHALPRPDDAGQPATRRAVAPRDETETLLAALWCEVLGLER
                     VSVLDNFFELGGHSLYAVQLFSRIRRRFGVEVTFQRFFEDPTISGMARLVAEGAKIRP
                     SGDEGIVAVPRGGPLPLSAAQERLWFLERLAPGTRAYNCSYYFRLRGPLDVVALRLAV
                     HALCARHEPLRTTFGEQDGRPFQSIAPEAGVDLALVDLSLLSEAEREEALARRLADEA
                     RQTFDIERGPLARVGAIRLGAEEHILTLHFHHLIIDGWSIGVAFRELGQLYAAARDGR
                     PAGLPPLPLQYADFAAWQRRQLAGPAYDRVLAWWCQRLADAPRVLELPVDAPRPAVQT
                     FEGGTEGFRLGPGLTDALRRLATAEGTTLATALLAVFSAVLCRMTGCDDLMLGVPSAN
                     RDRIELEGMLGFFVNTLPLRVSTGGDPPFRELLQRARQTTLAAYERDGVPFERIVQEL
                     RVSRSASMNPLVQVGFSPLPPAEVRLQLPGLASEYIEADSKKTVFDLSLYFWEVGDGA
                     EGLLEYSTDLFEPATIRRLAEHLQTLAAAAAAEPATRIGALPLMADAERHALVALRGV
                     PEPRAPGCVHTLFAAHAARTPDAPAVEQQGQVWSYRELDRRAQRLAGWLRARGVGPET
                     LVGCCLERSREWAVAVLAIWKAGGVYVPLDPNYPARRLQFLVEDAAPPVILTHSRLAP
                     RLPASDGLVVCLDLEAAAITDHVDEAPAVDVQPGQAAYVIFTSGSTGRPKGVVVEHRH
                     AVHLAHAQREAPELTAGARVLQFSALGFDASLWEILMALTTGGTLCLPPSGAALYGPD
                     LLHLLRDARISVVLLPPSLLSSLPQEPLPDLRLLFAGGEACPAEVVARWSAPGRRVVN
                     AYGPTEATVVATWFACRADGAAPPIGRPLRGVTVHVLDAAMQPVPIGVPGELYVGGNG
                     VARGYLGRPELTAERFLPDPFDAVPNARLYRTGDRVRWQADGSLQFLGRIDQQVKLRG
                     FRIELGEVEAVLREHPAVRDAVAVVRAGTGGDR"
BASE COUNT          543 a         1481 c         1448 g          414 t
ORIGIN      
        1 cgatcgccgc cggtgccggc gcggacgacc gccacggcgt cgcggaccgc cgggtgctcg
       61 cgcaacaccg cctcgacctc gccgagctcg atgcgaaacc cgcggagctt gacctgctgg
      121 tcgatgcgac ccaaaaactg caagctgccg tcggcctgcc agcgcacgcg gtcgccggtg
      181 cggtagaggc gggcgttcgg gaccgcgtcg aaggggtcgg gcaggaagcg ctctgcggtc
      241 aactcgggcc ggccgaggta gccgcgcgcg accccgttgc cgccgacgta cagctcgccc
      301 ggcacgccga tcggcaccgg ctgcatcgcc gcgtcgagca cgtgcaccgt cacgccccgc
      361 agcggccgac cgatcggcgg cgcggcgccg tcggcccggc acgcgaacca cgtggcgacc
      421 accgtcgcct cggtcggacc gtaagcgttg acgacccggc gcccgggcgc cgaccagcga
      481 gcgacgacct ccgcggggca cgcctcgccg ccggcgaaca gcaggcgcag gtccggcagc
      541 ggctcctgcg gcagcgacga cagcagcgag ggcggcagca ggaccaccga gatccgcgcg
      601 tcgcggagca gatgcagcag gtcgggcccg tagagcgcgg cgccggaggg cggcaggcac
      661 agcgtcccgc cggtcgtcag cgccatcagg atctcccaca gcgaagcgtc gaagccgagg
      721 gccgagaact gcagcacgcg ggcgcccgcc gtcagctccg gcgcctcgcg ctgcgcgtgc
      781 gcgaggtgga ccgcgtggcg gtgctcgacg accacgccct tgggccgacc ggtcgagccc
      841 gaggtgaaga tcacgtacgc cgcctgcccg ggctgcacgt cgaccgcggg cgcctcgtcg
      901 acgtgatcgg tgatcgccgc ggcctcgagg tcgaggcaga cgaccaggcc gtcgctcgcc
      961 ggcagccgcg gggcgagccg ggagtgggtc aagatgaccg gcggggccgc gtcctcgacc
     1021 aggaactgca gccggcgcgc cggatagttg gggtcgagcg ggacgtacac gccgccggcc
     1081 ttccagatcg ccagcaccgc gaccgcccac tcgcgcgagc gctcgaggca gcaaccaacg
     1141 agcgtctccg ggccgacgcc gcgggcccgg agccagccag cgagccgctg cgcgcggcgg
     1201 tcgagctcgc ggtacgacca cacctggccc tgctgctcga ccgcgggcgc gtcgggggtg
     1261 cgggccgcgt gcgcggcgaa cagcgtgtgc acgcagcccg gcgcccgcgg ctccggcaca
     1321 ccacgcaggg cgaccagcgc gtggcgctcc gcgtcggcca tcaagggcag cgcgccgatc
     1381 cgcgtcgctg gctccgccgc cgcggcggcg gccagggtct gcaggtgctc cgcgagccgg
     1441 cggatcgtcg ccggctcgaa caggtcggtg ctgtactcga gcaggccctc cgcgccgtcg
     1501 ccgacctccc agaagtacag cgacaggtcg aacaccgtct tcttggagtc ggcctcgatg
     1561 tactcgctgg ccagccccgg cagctgcagc cggacctccg cgggcgggag cggcgagaag
     1621 ccgacctgga ccagcgggtt catgctcgcc gaccgcgaca cccgcagctc ctggacgatc
     1681 cgctcgaacg gcaccccgtc gcgctcgtag gccgcgagcg tggtctgccg ggcccgctgc
     1741 agcagctcgc ggaacggcgg gtccccgccg gtcgagacgc gcagcggcag cgtgttgacg
     1801 aagaagccga gcatgccctc gagctcgatg cgatcgcggt tcgcgctcgg caccccgagc
     1861 atcaggtcgt cgcacccggt catccggcac agcaccgccg agaacaccgc cagcagcgcg
     1921 gtcgccagcg tcgtcccctc cgcggtcgcc agccggcgca gcgcgtcggt caatccgggc
     1981 ccgaggcgaa agccctcggt cccgccctcg aaggtctgga ccgccggccg cggcgcgtcg
     2041 accgggagct cgagcacgcg cggggcgtcg gcgaggcgct ggcaccacca cgcgagcacg
     2101 cggtcgtacg ccggcccggc cagctggcgg cgctgccacg ccgcgaagtc ggcgtactgc
     2161 agcggcagcg gcggcagccc ggcgggccga ccgtcgcggg ccgccgcgta gagctggccg
     2221 agctcgcgga acgcgacgcc gatcgaccag ccgtcgatga tcaggtggtg gaagtgcagc
     2281 gtgaggatgt gctcctccgc gccgaggcgg atcgcaccga cccgcgcgag cggaccgcgc
     2341 tcgatgtcga aggtctggcg cgcctcgtcg gcgagccgcc gggcgagcgc ctcctcgcgc
     2401 tcggcctccg acagcagcga caggtcgacc agcgcgaggt cgaccccggc ctccggcgcg
     2461 atgctctgga acggccgccc gtcctgctcg ccgaaggtcg tgcgcagtgg ttcatggcgg
     2521 gcgcacagcg cgtgcacggc gaggcgcagg gcgacgacgt cgagcgggcc gcgcaggcgg
     2581 aagtagtacg agcagttgta cgcgcgcgtc cccggggcca ggcgctcgag gaaccacagc
     2641 cgctcctgcg cggccgacag cggcagcggc ccgccgcgcg gcacggcgac gatgccctcg
     2701 tcgccgctcg gccggatctt ggcgccctcg gcgacgaggc gcgccatgcc ggagatcgtc
     2761 gggtcctcga agaagcgctg gaacgtcacc tcgacgccga accgccggcg gatccgactg
     2821 aacagctgca ccgcgtacag cgagtggccg ccgagctcga agaagttgtc gagcaccgag
     2881 acccgctcga gcccgaggac ctcgcaccac agcgcggcga gcagcgtctc cgtctcgtcg
     2941 cgcggggcga ccgcgcggcg cgtcgccggc tgaccggcgt cgtcggggcg cggcagcgcg
     3001 tggcggtcga ccttgccgtg cgccgtcaac ggcagcgcgt cgcacaccat gaaccgcgcc
     3061 gggatcgcgt actcgggcag gcgccgctgc agcgccgccc gcaactgctg gaccagcgcc
     3121 cgctcctgcg ccgcgcgcag cggatgattg gtgctcgcct cggtcgcctc ggccgcgggc
     3181 caccgccccg gcgccgccgt catcggctcg aacagcgcgt cgaccaggcc gagcccggcg
     3241 tccgagcgcc ggaccgtcac gcgccgaccg tgccgctcgc cgagcgagtg cagcgcctcg
     3301 gggtcgatcg ccggaccatt cgttacagcc tgcccgcgct cgaggcggtc ccacagcgcg
     3361 acgtcggcgg cgacccgcgc gttggggatc cccgcgacga tcagcgcgtc gtcgccggcg
     3421 gcgagccggg tcgccagccc gtcgaggccg cccggcagcg ccgaccaggc gagcgtgacg
     3481 gcctcgacgg cctcgaccgc gcacccgacg tgcagcgtga cgtcgtggcg gtagcgcagc
     3541 atctcgacgt cgccggcgcc gcgcttgagc gccacgctcg cgtggccgcc gagccgccga
     3601 gccagcgcgt ggaacagctc cggtgcgacg agcagctcct cctccgcgcg catcgcctcg
     3661 tggacgcggg cgcgcagctg cgccgggtcg acgtcgccga ggcggtgccg ctgcaccgac
     3721 gcgtggaacg gcccgagcag cccgtgatcg cggatatcgc cgaggaagat cgcgccgccg
     3781 gggcggaccg cccgggccgc gagcagcagc gtccgcgtca ggtactcggc gtccgggaag
     3841 tactggacca ccgagttgag gatcaccagg tcgaacggcg cctcgg
//
LOCUS       DASWNJ010000450         2597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_608246, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000450 DASWNJ010000000
VERSION     DASWNJ010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608246"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(479..1195)
                     /locus_tag="VGB85_07710"
     CDS             complement(479..1195)
                     /locus_tag="VGB85_07710"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY0133949.1"
                     /translation="MSKLLLIRHGQASLGAPDYDVLSPLGVVQARRLGTWLAAMQVRP
                     DRIVTGPRKRQRDTAAHLVATAREGGAAFPDPIEDVAFDEYPALEIMQRHLQRLAADD
                     AELGTHLEDLQRHAAATREHRRAFEATFQRMMRHWHDDRLGDPEIESCTGFQDRVRDG
                     LTALSQARGQTVLVVTSAGPVGVGLRLGLDLDPWSAIKSSFVVHNSSLTELRSRGSGP
                     ILTSFNALPHLHDPAEITLR"
     gene            complement(1247..2482)
                     /locus_tag="VGB85_07715"
     CDS             complement(1247..2482)
                     /locus_tag="VGB85_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133950.1"
                     /translation="MRAIFSSLLLLGLLPACGGDDGTTDDASTDDASTGATSNDPTGG
                     ATEPTTGEPADLNERELGAGASAQCLGAMTHAAKLTEAVAAGDAAAALAAYAGTDLQT
                     YVVAIDGAEGTHDAAITASLAIGDATELAAVEGRLHTALIQHLRAQLAAVEEGTEDHY
                     ATWDEAHCIWDGGLRGLATAAEAVGWTSVEEQIAADIDAGFQAGHDGIQGEPPSSGVD
                     DWQVPPNKQRVEKSLYRAIQRVIVELAVKARKGADPAPARHALELFASIESRLDGRNT
                     PGIAQVQAILGGDPAAIDPDAVLLELDIAFAKRTRTYASGAIDNDELGVPGGYEGAVE
                     GGVYAKLIVAGIVKSGKVADFDLDAYLTAWDRYAVLVRGGTEIEELNTVSKYLVDTTC
                     AYQTALGVAECSGNVDETE"
BASE COUNT          305 a          837 c         1043 g          412 t
ORIGIN      
        1 catctgggct cgctagtcct ctcgtgcggg tcgggttcga tcagggggag ttcggcgtga
       61 tgcgagcgcc gggcctggcg aggccgggga gcgcggggcg aggctgcctg ggccggggag
      121 gggccgacgg gctacggggc cctgcgacgc gggtcctccc ggggcacgtg ctcgccgccc
      181 cacctgtccg gaagaacatg tctctttcac catggctgtc tctccgctcg tgcgcggcgg
      241 tggggcggct gcgcgcgagc cccaggtcct cccgctgctt cccccgccag tcggcccctg
      301 ctccccgggc ggacgctgtc tgcgccccgc tcgtggccgt gccggtgctg tcgaggaccc
      361 gaacgtcggc gtgtcaggcc gcgcgagccc ggtgatgttg gatgctgctc cgggcttgcg
      421 acgcccgagc ggccggggag gtcgtggggg tcgtcgagca tgtgcgcctc ggtgcggact
      481 accgcagggt gatctcggcg gggtcgtgca ggtgggggag ggcgttgaag gaggtgagga
      541 tcgggccgct gccgcggctg cgcagctcgg tcagcgagct gttgtggacg acgaagctgg
      601 acttgatcgc cgaccagggg tcgaggtcga ggccgaggcg caggccgacg ccgaccgggc
      661 cggccgaggt gacgacgagg accgtctggc cgcgcgcctg cgacagggcg gtgaggccgt
      721 cgcgcacgcg gtcctggaag ccggtgcagc tctcgatctc ggggtcgccg aggcggtcgt
      781 cgtgccagtg tcgcatcatg cgctggaacg tcgcctcgaa ggcccggcgg tgctcgcggg
      841 tcgccgcggc gtggcgttgc aggtcttcga ggtgggtgcc gagctcggcg tcgtcggccg
      901 cgaggcgttg caggtggcgc tgcatgatct cgagcgccgg gtactcgtcg aaggcgacgt
      961 cctcgatcgg gtcggggaac gcggccccgc cctcgcgggc ggtggcgacg aggtgggccg
     1021 cggtgtcgcg ctggcgcttg cgcgggccgg tgacgatccg gtcggggcgc acctgcatcg
     1081 ccgcgagcca ggtgccgagg cggcgggcct ggacgacgcc gagcggtgag agcacgtcgt
     1141 agtcgggcgc cccgagggag gcctggccgt ggcggatcag cagcagcttg ctcacgcagg
     1201 agcgagcttg gcgaatgtcc tggatccggt caaccgccgc gagccgctac tcggtctcgt
     1261 cgacgttgcc cgagcactcc gcgacgccga gggcggtctg gtaggcgcag gtggtgtcga
     1321 cgaggtactt gctgacggtg ttgagctcct cgatctcggt gccgccgcgg acgagcacgg
     1381 cgtagcgatc ccaggcggtg aggtaggcgt cgaggtcgaa gtcggcgacc ttgccggact
     1441 tgacgatgcc ggcgacgatc agcttcgcgt agacaccgcc ctcgacggcg ccctcgtagc
     1501 cgccggggac gccgagctcg tcgttgtcga tcgcacccga ggcgtaggtg cgcgtgcgct
     1561 tggcgaaggc gatgtcgagc tcgagcagga cggcgtccgg gtcgatcgcg gccgggtcgc
     1621 cgccgaggat cgcctggacc tgggcgatgc ccggggtgtt gcggccgtcg aggcggctct
     1681 cgatgctggc gaacagctcg agcgcgtggc gggccggggc cgggtcggcg cccttgcgcg
     1741 ccttgacggc gagctcgacg atgacgcgct ggatggcgcg gtacagcgac ttctcgaccc
     1801 gctgcttgtt cggcgggacc tgccagtcgt cgacgccgga gctcggcggt tcaccctgga
     1861 tgccgtcgtg accggcctgg aagccggcgt cgatgtcggc ggcgatctgc tcctcgacgc
     1921 tcgtccagcc gacggcctcg gcggcggtgg cgaggccacg caggccgccg tcccagatgc
     1981 agtgggcctc gtcccaggtc gcgtagtggt cctcggtgcc ctcctcgacg gcggccagct
     2041 gggcgcgcag gtgctggatc agcgcggtgt gcaggcgacc ctcgacggcg gcgagctcgg
     2101 tggcgtcgcc gatcgcgagg gaggcggtga tcgcagcgtc gtgcgtgccc tcggccccgt
     2161 cgatcgcgac gacgtaggtc tggaggtcgg tgcccgcgta ggccgcgagc gctgccgcag
     2221 cgtcaccggc agcgaccgcc tccgtgagct tggccgcgtg agtcatcgcg ccgagacact
     2281 gcgcgctggc gcctgcgccc agctcacgct cgttgaggtc cgccggctcg cccgtggtcg
     2341 gctcggtggc gccgccggtc gggtcgttgc tggttgcgcc ggtgctggcg tcgtcggtgc
     2401 tggcgtcgtc agtggtgccg tcgtcgccgc cgcaggcggg aaggaggccg agaagaagga
     2461 gggaagagaa gatcgctcgc atggaaggct ccgggcgctg ggcgcctccg gtgtgatatt
     2521 gaaagccttt ttcaattaca agcgtaacga aagtcgccca tcccggggct tgaccccctg
     2581 tataacccgg tgctaga
//
LOCUS       DASWNJ010000451         5012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_149084, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000451 DASWNJ010000000
VERSION     DASWNJ010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149084"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..935)
                     /locus_tag="VGB85_07720"
     CDS             complement(114..935)
                     /locus_tag="VGB85_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133951.1"
                     /translation="MNIKQLVSHACSFSVILAAAAVPGCNNEAPVGDLTVPFEIGAGI
                     ECSLKNVVDVKVTLLEIKSGEAEEEEVESTSVPCSEGKAIFNNIPVGTYQIRVEGFDP
                     DEFVVVDNVPKIEDGVVEKLEVGEVLEGKETTTEIINLSSTPAKLWVRYELNMEGFQT
                     MCSQIKMTELAISAFKNDGADQILTITLPCDATPNESNGYHYLSDPERELDGSVFDYV
                     RVQPRDATGNMTGTDVKFALAMPPGAGRTVKLTFNAECTADKCDLQCIGGACTPD"
     gene            complement(1101..3152)
                     /locus_tag="VGB85_07725"
     CDS             complement(1101..3152)
                     /locus_tag="VGB85_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133952.1"
                     /translation="MSEETAPTYRPAWPDPLGLRRDELAELGLLLPGVAVLPILHGRL
                     EFAWMVRKAIEKLSPAALAVELPESVGDAFRRAVGRLPLLSVVEYTDSVGRPVYLPVE
                     PADALVETVRRGLELGIPVHLVDRDVDHYPQVRDRLPDALAAESVGARIFLQTALECA
                     ALNPRVSDDETREQTIAHNVAAVVRARPPGHADSLVLVVCGLHHARGVVEQLRSLLAD
                     GLLPATPLRRVKREGVRVHHLGEASSREVLAELPFVNAAYERSRGTAPPSAQGPAHAA
                     TVVDLFTRARQAPKIDPFRTAAPPEPPEDRPRATGRMGLLLSLCQEARKVHQERTGEA
                     LRPGSLLQLLRWACRYAWLEGGLGPDLYQLVIAARGFADDNYAHEVWEVATHYPWQTT
                     RPELAPLDISLRDLHDRVRTIRFHPKTLQRRHRLMRVIKPRPKEKRPGEWAEQFDGDS
                     MCSYPPEDLALEHYGAFLRKRTERVLSASQTIVEPFTTNFYDGVDVRETVRNWHSGTL
                     YVRRELAIKGKPGAVVVIFEDEPITPQEAEEPKFPWHMTWQGEHEEEGDMALYSTDPF
                     ERVVGPGIGRALYGGFLLTRPPGTMFGVWEDPFYQDARSKAERLLLAALDHTPERLVV
                     YVAPAPPRQSLRQLAQRMDRKIVHVPLGQLSPITLKRLRVFHVLSDRSARASAREYIR
                     D"
     gene            complement(3154..4068)
                     /locus_tag="VGB85_07730"
     CDS             complement(3154..4068)
                     /locus_tag="VGB85_07730"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0133953.1"
                     /translation="MDSVVIQGVELRLTPPDSLEWEWVGRPELRRQLLACWMLLDDAD
                     LPLSPRLVGKPGVGKTTLAAAAAKSLGLPLYVFQATMDTRPEDLLVTPVIADGQKLRY
                     IASPLVSAMITGGVCVLDEGNRMSEKSWASLAPLLDRRRYVESIVAGIKIPAHKQFRV
                     CATMNDDSSTFDIPEYIHSRLQPQILIDFPERDEELAILQANLPFAQSRILHYLSVFL
                     QRAHEDDQPYTVRDGINIARYAMKLGASERGGLDIKKHVRHATQQILGEEALEFLPGE
                     VPIDPASGPPPPPSSSPPPPIKPSPFKR"
     gene            4290..>5012
                     /locus_tag="VGB85_07735"
     CDS             4290..>5012
                     /locus_tag="VGB85_07735"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY0133954.1"
                     /translation="MRPRLVLATLACLLFGQPAAADANYPITLPSQPGEEEPGGPDLG
                     EPGPGSPDPVESEPDEAEEQAPVDTRELKPVAKPKVAPKVSKPRPAKKSKVKGDAACD
                     FRVPVYEHEVIDGDIASSIAARYGVRLGEIKKLNPGLDINKLRIGKKIRVCPDIAPRL
                     REEFTYTVKSGDNLSKIGEKYGLLPKEIVRLQRGKLRQRLEANPNSVKEGDELTLVVD
                     RGVLPEFAPRKNEDRGTLKVGVV"
BASE COUNT          740 a         1523 c         2009 g          740 t
ORIGIN      
        1 cacagacacc tcgccgcggg caggtgcgat taacgacaaa gggcgcgatc cttgcggata
       61 cgcgcccact tcggggcgaa aacgagacgc ccgcgtgatc tacgtgcgcg cgatcagtcg
      121 ggggtgcagg cgccgccgat gcactggagg tcgcacttgt cggcggtgca ctcggcgttg
      181 aaggtcagct tgacggtgcg gccggccccc ggcggcatgg cgagcgcgaa cttgacgtcg
      241 gtgccggtca tgttgccggt cgcgtcgcgc ggctggacgc gcacgtagtc gaacacggag
      301 ccgtcgagct cgcgctcggg gtcgctgagg tagtggtacc cgttggactc gttcggggtc
      361 gcgtcgcagg ggagggtgat cgtcaggatc tggtcggcgc cgtcgttctt gaaggcggag
      421 atcgcgagct cggtcatctt gatctgcgag cacatggtct ggaagccctc catgttgagc
      481 tcgtagcgga cccacagctt ggccggggtc gacgagaggt tgatgatctc ggtggtggtc
      541 tccttgccct cgagcacctc accgacctcg agcttctcga cgacgccgtc ctcgatcttg
      601 gggacgttgt cgacgacgac gaactcgtcg gggtcgaagc cctcgacgcg gatctggtag
      661 gtgccgaccg ggatgttgtt gaagatggcc ttgccctcgc tgcaggggac ggaggtcgat
      721 tcgacctcct cctcctcggc ctcgccgctc ttgatctcga gcagcgtcac cttgacgtcg
      781 acgacgttct tgagcgagca ctcgatgccg gcgccgatct cgaaggggac ggtcaggtcg
      841 ccgaccgggg cctcgttgtt acagccgggg acggcggccg cggcgaggat gacggagaag
      901 gaacaggcgt gggagacaag ctgcttgatg ttcatggacg tccttgagcg ggcggcaggg
      961 acgcggcgga ccccggcgat gttcacgggc ggtgcgccga ggcggccgcc cggcgaagag
     1021 atagccgagg ccggggcttt gggccaagtt tcggcgatgc tgcggggcga aaaagccggg
     1081 ccgggggccg gcgtgtgctt ttagtcgcgg atgtactcgc gcgcgctggc gcgggcgctg
     1141 cggtcactga gcacgtggaa cacgcgcagg cgcttgaggg tgatcggtga gagctggccg
     1201 agcgggacgt ggacgatctt ccggtccatg cgctgagcca gctggcgcag gctctggcgc
     1261 ggcggagcgg gcgccacgta gacgaccagg cgctcggggg tgtggtcgag ggcggcgagc
     1321 agcaggcgct cggccttgct gcgggcgtcc tggtagaacg ggtcctccca cacgccgaac
     1381 atggtgcccg gcgggcgggt cagcaaaaag ccgccgtaga gggcgcggcc gatgccgggg
     1441 ccgacgacgc gctcgaaggg gtcggtcgag tagagggcca tgtcgccctc ctcctcgtgc
     1501 tcgccctgcc aggtcatgtg ccacgggaac ttgggctcct cggcctcctg gggggtgatc
     1561 ggctcgtcct cgaagatgac gacgacggcg ccgggtttgc ccttgatcgc cagctcgcgg
     1621 cgcacgtaga gggtgccgga gtgccagttg cgcacggtct cgcggacgtc gacgccgtcg
     1681 tagaagttgg tggtgaacgg ctcgacgatc gtctgcgagg cgctgaggac ccgctcggtg
     1741 cgcttgcgca ggaaggcgcc gtagtgctcg agcgcgagat cctcgggcgg gtaggagcac
     1801 atgctgtcgc cgtcgaactg ctcggcccac tcgccggggc gcttctcctt ggggcgcggt
     1861 ttgatgaccc gcatcaggcg atggcgccgc tgcagggtct tcgggtggaa gcggatcgtg
     1921 cggacgcggt cgtgcaggtc gcgcaggctg atgtcgagcg gggcgagctc ggggcgggtg
     1981 gtttgccagg ggtagtgggt cgcgacctcc cagacctcgt gggcgtagtt gtcgtcggcg
     2041 aagccgcggg cggcgatgac gagctggtag aggtcggggc cgaggccgcc ctcgagccag
     2101 gcgtagcggc aggcccagcg cagcagctgc aacaggctcc cggggcgcag ggcctcgccg
     2161 gtgcgctcct ggtggacctt gcgggcctcc tggcacagcg agagcagcag gcccatgcgg
     2221 ccggtcgcgc gcggtcggtc ctcgggcggc tcgggcgggg cggcggtgcg aaacgggtcg
     2281 atcttcgggg cctggcgggc cctggtgaac aggtcgacga cggtggcggc gtgggcgggg
     2341 ccctgggcgg agggcggggc ggtgccgcgg ctgcgctcgt aggcggcgtt gacgaagggc
     2401 agctcggcga gcacctcgcg gctcgaggcc tcgccgaggt ggtgcacgcg cacgccctcg
     2461 cgcttgaccc ggcgcagcgg ggtggcgggc agcaggccgt cggcgagcag gctgcgcagc
     2521 tgctcgacga cgccgcgggc gtggtgcagg ccgcagacga cgagcacgag cgagtcggcg
     2581 tggccggggg gccgggcgcg cacgaccgcg gcgacgttgt gggcgatcgt ctgctcgcgg
     2641 gtctcgtcgt cggacacgcg agggttgagc gcggcgcact cgagggcggt ttggaggaag
     2701 atgcgggcgc cgaccgactc ggcggcgagg gcgtcgggca ggcggtcgcg gacctgcggg
     2761 tagtggtcga cgtcgcggtc gacgaggtgc acgggaatac cgagctcgag gccgcggcgc
     2821 acagtttcta ccagggcgtc ggcgggctcg accgggaggt agacgggccg gccgacggag
     2881 tcggtgtact cgacgacgct gagcagcggg aggcggccga cggcgcggcg aaacgcgtcg
     2941 ccgacgctct cgggcagctc gacggcgagg gcggccgggc tgagcttctc gatcgccttg
     3001 cggaccatcc aggcgaactc gaggcggccg tggaggatcg ggaggaccgc gacgccgggc
     3061 agcaggaggc cgagctcggc cagctcgtcg cggcgcaggc cgagcgggtc cggccacgcc
     3121 gggcggtagg tcggggctgt ctcttcggac atgtcagcgc ttgaacggtg acggcttgat
     3181 cggcggcggg ggggacgacg acgggggcgg cggcgggccg gaggcggggt cgatcgggac
     3241 ctcgccgggc aggaactcga gcgcctcctc gccgaggatc tgctgggtgg cgtggcggac
     3301 gtgcttcttg atgtcgaggc cgccgcgctc gctggcgccg agcttcatcg cgtagcgggc
     3361 gatgttgatg ccgtcgcgca cggtgtaggg ctggtcgtcc tcgtgggcgc gctgcaggaa
     3421 caccgagagg tagtggagga tccgcgactg ggcaaagggg aggttggcct ggaggatcgc
     3481 cagctcctcg tcgcgctcgg ggaagtcgat caggatctgc ggctggagcc ggctgtggat
     3541 gtactcgggg atgtcgaagg tgctcgagtc gtcgttcatc gtcgcgcaca cgcgaaactg
     3601 cttgtgcgcg gggatcttga tgccggcgac gatcgactcg acgtagcggc ggcggtcgag
     3661 cagcggcgcg agcgaggccc agctcttttc actcatgcgg ttgccctcgt cgagcacaca
     3721 cacgccgccg gtgatcatcg cgctgaccag cgggctggcg atgtaacgca gcttttgccc
     3781 gtcggcgatc acgggggtga cgagcaggtc ctcggggcgc gtgtccatcg tcgcctggaa
     3841 cacgtagagc ggcaggccga gcgacttggc ggcggcggcg gcgagggtgg tcttgccgac
     3901 gccgggcttg ccgacgaggc gcggggacag cgggaggtcg gcgtcgtcga gcaacatcca
     3961 gcaggcgagc agctgcctgc gcagctcggg tcggccgacc cactcccatt cgagcgagtc
     4021 aggcggggtc aggcgcagct cgacgccctg gatcacgaca gagtccattg ccggggggta
     4081 taacaggggg cgcggtgggg ccgcgagaaa actccaggtc gctgggggtt ggtgatggca
     4141 aaaccggcat ggacggggcc gcggggccgc cgcggggagc gcgctacgat cacgaagttg
     4201 acatcgaggg cgggtcggcc tgaataaagc acccttcacg gggtctagct gcccgcgggc
     4261 cgctcgggcc cggaccggag ctcccccgca tgcgcccacg cctcgtcctc gccacgctcg
     4321 cctgcctcct cttcgggcag cctgcggccg ccgacgcgaa ctacccgatc accctgccgt
     4381 cgcagccggg cgaggaggag ccgggcgggc cggacctcgg ggagccgggc ccgggcagcc
     4441 cggacccggt cgagagcgag cccgacgagg cggaggagca ggcgccggtc gacacacgtg
     4501 agctcaagcc ggtggcgaag ccgaaggtcg cgccgaaggt gagcaagccg cggccggcga
     4561 agaagtcgaa ggtcaagggc gatgcggcct gcgattttcg ggtgccggtg tacgagcacg
     4621 aggtgatcga cggcgacatc gccagcagca tcgcggcccg ctacggcgtg cggctcggtg
     4681 agatcaagaa gctcaacccg gggctcgaca tcaacaagct gcggatcggt aagaagatcc
     4741 gggtgtgccc ggacatcgcg ccgcgcttgc gtgaagagtt cacctacacg gtcaagagcg
     4801 gcgataacct gtcgaagatc ggtgagaagt acggcctgct gccgaaggag atcgtccgcc
     4861 tgcagcgcgg caagctgcgc cagcggctcg aggccaaccc caacagcgtc aaggaaggcg
     4921 acgagctgac gctggtggtc gaccgcggcg tgctccccga gttcgcgccg cggaagaacg
     4981 aggaccgcgg cacgctcaag gtcggtgtcg tg
//
LOCUS       DASWNJ010000452         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_220199, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000452 DASWNJ010000000
VERSION     DASWNJ010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220199"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VGB85_07740"
     CDS             complement(<1..291)
                     /locus_tag="VGB85_07740"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0133955.1"
                     /translation="MTRAPAASLVWAVMIAVFLAAVDQTIVATALPRISGELGDAALY
                     AWVFTSYMMATTVFVPIAGTIGDTHGRRPVLLFGVIVFAIGSLLAGAATSMSQ"
     gene            complement(288..1493)
                     /locus_tag="VGB85_07745"
     CDS             complement(288..1493)
                     /locus_tag="VGB85_07745"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY0133956.1"
                     /translation="MSRARFLATLAVQVPWTAAQLLVEALLLASVARRPARAGERPWA
                     ERYERIGPGWLTGILRDGGALDGGEVVAVELERFGTVGQMSAVFRLRPTYGPGAAGPE
                     RLVLKTTAPEMKQRVLNAALGVFAEELRCYRLPRPERGLLRPHCWYAAQHRLTRAAIL
                     VIDDLSAWRGAPADGQLAVEDATRVVLALAEHHATWWQDPGLRARGFKTTSKMVCDTF
                     GPMCALAWPRARKLMTPLVDADTLALLGGYVTHHEAIGRRIMAGPVTLVHGDLNPNNL
                     FLDDAGGAVYAIDWQAAHIGHWAEDLAYLAVMSMDAEVWAAHEDAMIAAHRGVLVDRG
                     IAVDEARHRADYALGLFQVGAILILAALILDPRKNPPLYEQYRERIRGWAIAARRHRL
                     PALLAEVQR"
     gene            1776..>2432
                     /locus_tag="VGB85_07750"
     CDS             1776..>2432
                     /locus_tag="VGB85_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133957.1"
                     /translation="MLIRSANIVGPMSYSTTTLPSLRWLLAVPLTSSPSGRVVPLADV
                     FEHMPIAPELDLGPAVGADGPRRVVVQFPRLRSFSRAAVLAAAPLLASLQALAQRLAA
                     DPGTAPADVLASVAGLVGEGPLHAELAAIFAGKPQVTAEPAAAAPVGDTRSLVDELLD
                     RATTSATAVDRPASIIDAIVRSGRPRPDAHTKTAAQAARERILAAADRGADAALADPR
                     V"
BASE COUNT          328 a          914 c          873 g          317 t
ORIGIN      
        1 ctggctcatc gaggtcgccg cgccggcgag cagggagccg atcgcaaaca cgatcacgcc
       61 gaacagcagc accgggcggc gtccgtgcgt gtcgccgatc gtgcccgcga tcggcacgaa
      121 cacggtggtc gccatcatgt acgaggtgaa cacccaggcg tagagcgcgg cgtcgccgag
      181 ctcgccggag atccgcggca gggcggtggc gacgatcgtc tgatcgacgg cggcgaggaa
      241 cacggcgatc atcaccgccc acaccaggct cgccgccggc gctcgggtca tcgctggacc
      301 tcggcgagca gcgcgggcag gcgatgccgg cgcgccgcga tcgcccaccc gcggatccgc
      361 tcgcggtatt gttcgtacag cggcggattc ttgcgtggat cgaggatcag cgcggcgagg
      421 atgagaatgg cgccgacctg aaacaggccg agagcatagt cggcccggtg tcgcgcctcg
      481 tcgacggcga tcccacgatc gacgagcacg ccgcggtgcg cggcgatcat cgcgtcctcg
      541 tgcgcggccc agacctccgc gtccatgctc atgacggcga ggtaggcgag gtcctccgcc
      601 cagtggccaa tatgcgcggc ctgccagtcg atcgcatata cggccccgcc ggcgtcgtcg
      661 aggaacaggt tgttcgggtt gaggtcgccg tgcacgagcg tcaccggacc ggccatgatc
      721 cggcggccga tcgcctcgtg gtgggtcacg tagccgccga gcagcgcgag cgtgtcggcg
      781 tcgacgagcg gcgtcatcag cttgcgggcc cggggccagg cgagcgcgca catcggaccg
      841 aaggtgtcgc acaccatctt tgaagtggtc ttgaagccgc gcgcccgcag cccgggatcc
      901 tgccaccagg tcgcgtggtg ctcggcgagc gcgagcacca cccgcgtggc atcctccacc
      961 gcgagctgcc cgtccgcggg cgcgccgcgc cacgccgaga ggtcgtcgat cacgaggatc
     1021 gccgcgcgcg tgaggcggtg ctgcgccgcg taccagcagt gcggccgcag caggccgcgc
     1081 tcgggtcgcg gcaggcgata gcagcggagc tcctcggcga acacgccgag ggccgcgttg
     1141 agcacgcgct gcttcatctc cggcgccgtc gtcttgagca cgagccgctc cgggcccgcc
     1201 gcgccgggtc cgtaggtcgg ccgcaggcga aacaccgccg acatctggcc gacggttccg
     1261 aagcgctcga gctcgaccgc gacgacctcg ccgccatcga gcgccccgcc gtcgcgcagg
     1321 atcccggtga gccagcccgg cccgatccgc tcgtaacgct cggcccacgg ccgctccccc
     1381 gcacgcgccg gccggcgcgc gacgctggcg agcagcagcg cctcgaccag cagctgtgcg
     1441 gcggtccacg ggacctgaac ggcgagggtg gcgaggaaac gagcgcggga catcgcgggg
     1501 ccggagtgtc ccacggcgag accggcctcc gcaaaccttg ccggccgacc cctgcgtggc
     1561 cccgccgcgt cccttcgtgc ccggaacgga cctcatgaca atcaccccgg cagtgctcgg
     1621 tgcccgatgg cgcgggtcgt gcgggccgtg ctgatcgccg cgacgccccg aggcgccacg
     1681 cgttcgtcgg tgcatacatt cgcgcgccat tatctgcgta tccgcgccgc gcacgggcgc
     1741 gccagtggtc ccgtgcgcgg cgacgatcgt cgacgatgct catccgcagt gcgaacatcg
     1801 tcggcccgat gagctactcg accacgaccc tgcccagcct gcgctggttg ctcgccgtcc
     1861 cgctgacctc ctcgccgagc gggcgcgtcg tgccgctggc tgatgtgttc gagcacatgc
     1921 cgatcgcacc ggagctcgac ctcgggccgg ccgtcggcgc cgacgggccc cgtcgtgtcg
     1981 tcgtgcagtt cccccggctg cgctcgttct cccgcgccgc cgtgctcgcc gcggcgccgt
     2041 tgctcgcctc gttgcaggcg ctcgcgcagc gcctcgccgc cgacccgggc accgcgccgg
     2101 ccgacgtgct ggcgagcgtc gcggggctcg tcggtgaggg cccgttgcac gccgagctcg
     2161 cggcgatctt cgccggcaag ccgcaggtga cggcggagcc cgccgcggcg gcgccggtcg
     2221 gggacactcg ctcgctagtc gacgagctgc tcgaccgcgc cacgacctcg gccacggccg
     2281 tcgatcgacc ggcgtcgatc atcgacgcga tcgttcgcag cggtcggccg cggccggacg
     2341 cccacacgaa gacggcggcg caggcggccc gcgagcgcat cctcgccgcg gcggatcgcg
     2401 gcgccgacgc ggcgctcgcc gacccgcgcg tg
//
LOCUS       DASWNJ010000453         5217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_360475, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000453 DASWNJ010000000
VERSION     DASWNJ010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360475"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VGB85_07755"
     CDS             complement(<1..470)
                     /locus_tag="VGB85_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133958.1"
                     /translation="MSSPSTPPRAQPAAAEHRQSSLVRLLPEAPVGWWDRAAWLTIAA
                     VLVLAVATFRDFGVTMDEAPHLGYGAHILHWYTSGFTDEGALTYRANYYYGGGYDLLG
                     AIFRRIAAPMQDVIALHLLGCLVGVLGLVGTWKLGRLLGGPRAGFLALGIVTLNP"
     gene            complement(563..1555)
                     /locus_tag="VGB85_07760"
     CDS             complement(563..1555)
                     /locus_tag="VGB85_07760"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEY0133959.1"
                     /translation="MATAVLLACREDPYVAPAASDLAVQNAACEGCHIPEAVQWRQSL
                     HRASFSNDAFARSFAREPEPFCQGCHAPHADPKRPPPAWAAENGVTCIACHGAEVVRA
                     AATPRSRDPAPHALLRGGEFAVSGACASCHEFDFPVSRQHPPGRKMQRTASEHARSEF
                     ADVGCAACHMASTQGRRDHRFAASRDPELLRAAISVEARRAGADLVQLRLAPVGVGHA
                     FPTGDLFRRIELRVEARDSHGELAGKAIRHLARHFPARVYTGPDAGRPDPLEPDDRLT
                     GPTSVEITVPGATLEHFLKWRVTYQRVDRRDPIDPERSSLAGAVVLAEGTLAGP"
     gene            complement(1582..2925)
                     /locus_tag="VGB85_07765"
     CDS             complement(1582..2925)
                     /locus_tag="VGB85_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133960.1"
                     /translation="MSAVAETRKIALCFAALSFACTANPRPQEATPDDPALPVTPVIK
                     TVVPAAAKVEAAPPVVAAGEAKPVEPVAVAVAQPVGDAPPVAAPPGAWPAPVAVEGVE
                     HLAPAWESAMKLDKPVALETLAGGVLLRVDGVPQELAPDGALVEKRTVDVPTKQGTMR
                     TDVEGVWPDDAWSIKVHYGDRAGSDVAFHKWRGGNRWVPQVSEGKKLWDGEELAGYQW
                     TPRGGMLLVTQDDGVASFMRLAGKHAAPQPMTFGSGYVRDAFESAGGMVFLFVRPEEV
                     DRQGSLEILRSCKPDEAPGCERSGGLTLDVVAPDRHHLGVMAARNRWSISVVVNRTIG
                     GPDGDHEEHAIVHYETGGWKIEAVPGNGDVRQMLAAPDGGLWIVTDPKPQSLWHRSDA
                     GKWVAVDLPAELAGAQELQVALRDPTHVWLAGNVGDVHAIHAAPAALQAAPSPEG"
     gene            3064..4266
                     /locus_tag="VGB85_07770"
     CDS             3064..4266
                     /locus_tag="VGB85_07770"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0133961.1"
                     /translation="MPTRRRFLEMTGALGLLAATGCARRGAPTTPPEREAPRPVTPRS
                     LLVLGGTGFIGPHLVRHAVARGHRVTIFTRGKREAELPASVERLIGDRNGKLEALAGR
                     RWDLVVDDSATNPDYVRQSTELLADKVGRYLFTSSTGVYYPYLEPGADESRPVRMTAQ
                     DPEDGAEAYGVAKAQCERHVLDTFNKNRNDGGVIVRPTYIVGPGDTTDRFPYWPQRLQ
                     RGGEVLAPGRRDDPAQLVDVRDLAEFMLKLLEDDRSGIYNVAGPAEPLTISQFLETAV
                     TTLNPDARLTWIDDYEFLAAHEIFDSIPWVMQRGNDEHCMEIRNDRAIAAGLKFRPLA
                     TTLRDTLAWWPTVPEARRTSPGFSLKPEQEAKALADWHARAQGRAGAAVSRLAQRERG
                     PRPRPYTF"
     gene            complement(4263..5030)
                     /locus_tag="VGB85_07775"
     CDS             complement(4263..5030)
                     /locus_tag="VGB85_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133962.1"
                     /translation="MTVCKRVVGGLVMMSATLAAVCPQTAEANVVEHLDLAEMLVATV
                     LPAVNFYGDPTKITWEGFNGLDYSTNRSKCATLVTQLFERAYDPDYVAWFGCTSPHAA
                     SYHDKIEVEDGFTLIESIHDVQPGDIIAIEYIDAGCTNLTCGTFSSCTSTGHVAIVAD
                     FPKARTASAPLVAGTLQFSVDIIDTSTSYHGTADTRYMSDAGGAHDQGVGQGTMRLYV
                     NGKDATHPIVGHSWSTSSGSAFFSNAKRDVVIGRYNG"
BASE COUNT          789 a         2000 c         1693 g          735 t
ORIGIN      
        1 ggattcaacg tcacgatgcc gagcgccagg aagcccgccc gcggcccgcc gagcagccgc
       61 ccgagcttcc acgtaccgac gaggccgagc acgccgacga ggcagccgag caggtgcagc
      121 gcgatcacgt cctgcatcgg cgccgcgatc cggcgaaaga tcgcgccgag caggtcgtag
      181 ccgccgccgt aataatagtt cgcgcggtag gtcagcgccc cctcgtccgt gaagccggac
      241 gtgtaccagt ggaggatgtg cgcgccgtag ccgaggtgcg gcgcctcgtc catcgtcacg
      301 ccgaagtcgc ggaaggtcgc caccgcgagc accagcaccg ccgcgatcgt cagccacgcc
      361 gcgcggtccc accagcccac cggcgcctcc ggcagcagcc gcacgagcga ggactgccgg
      421 tgttcagcag ccgcgggctg cgcgcgtggc ggggtggacg gcgacgacat ggcctcgaaa
      481 gcggcgcaac ctttgcccgc agcccgccac gctgtcaacc tcgccggtcc gcgccgtcgg
      541 ccgcgtccgc gcccggccga cctcacggtc ccgcgagcgt gccctcggcg agcaccaccg
      601 ccccggccag cgacgagcgc tccgggtcga tcgggtcgcg gcggtcgacg cgttgatacg
      661 tgacccgcca ttttaaaaaa tgctcgagcg tcgcgccggg caccgtgatc tcgacgctgg
      721 tcggtcccgt caggcggtcg tccggctcga gcgggtcggg ccggcccgcg tccggtccgg
      781 tgtacacgcg cgccggaaag tgacgcgcca ggtggcggat cgccttgcct gcgagctctc
      841 cgtggctgtc gcgcgcctcg acacgcagct cgatccggcg aaacaggtcc ccggtcggga
      901 aggcatgccc gacgcccacc ggcgccagtc gcagctgcac gaggtcggcc ccggctcgcc
      961 tcgcctcgac cgagatcgcg gcgcgcaaca gctccgggtc gcgcgaggca gcgaagcgat
     1021 gatcgcggcg cccctgcgtg ctcgccatgt ggcacgccgc gcagccgacg tccgcgaact
     1081 cggagcgcgc gtgctcgctc gccgtccgct gcatcttgcg ccccggcggg tgctggcgcg
     1141 acaccgggaa gtcgaactcg tggcagctcg cgcacgcgcc actcaccgcg aattcaccgc
     1201 cacgcagcag cgcgtgcggg gccggatcgc gcgagcgagg cgtcgccgcg gcgcgcacga
     1261 cctccgcccc gtgacacgcg atacacgtga ccccattctc cgccgcccac gccggcggcg
     1321 gtcgcttcgg gtcggcgtgg ggcgcatgac agccctgaca aaacggctcg ggctcgcggg
     1381 cgaacgaccg cgcgaatgca tcatttgaaa atgatgcacg gtgcagcgac tgccgccatt
     1441 ggaccgcctc cgggatatgg caaccctcgc aggcggcgtt ctgcaccgcg agatcgctgg
     1501 cggccggcgc gacatacggg tcctcgcgac acgccagcag gaccgcggtc gccaataccc
     1561 ccatcccgcg caacgcaccc attatccctc ggggctcgga gcggcctgca gcgccgcggg
     1621 tgcggcgtgg atcgcgtgca cgtcaccgac gttgccggcg agccacacat gcgtcgggtc
     1681 gcgcagcgcc acctggagct cctgcgcgcc ggcgagctcc gccggcaggt cgaccgccac
     1741 ccacttgcca gcgtcgctgc ggtgccacaa cgactgcggc ttcgggtcgg tcacgatcca
     1801 cagcccgccg tccggcgccg cgagcatctg gcggacatcc ccattgcccg gcaccgcctc
     1861 gatcttccag ccgccggtct cgtaatgcac gatcgcgtgc tcctcgtggt caccgtccgg
     1921 accgccgatg gtgcgattga cgacgacact gatgctccag cgattgcgcg ccgccatcac
     1981 gcccaggtga tgccggtccg gcgcgaccac gtccagcgtg aggccgccgc tgcgctcgca
     2041 cccgggggcc tcgtcgggct tgcagctgcg aaggatctcg aggctgccct ggcggtccac
     2101 ctcctcgggc ctcacgaaca agaacaccat gccgcccgcg ctctcgaacg cgtcgcgcac
     2161 ataaccgctc ccgaaggtca tcggctgcgg cgccgcgtgc ttgccggcca ggcgcatgaa
     2221 gctcgccaca ccgtcgtcct gcgtgaccag caacatcccc ccgcgcggcg tccactgata
     2281 tccggccagc tcctcgccgt cccacagctt tttgccctcg ctcacctgcg ggacccagcg
     2341 attcccgccg cgccacttgt gaaacgcgac gtcggagcca gcgcgatcgc cgtagtgcac
     2401 cttgatcgac cacgcgtcgt cgggccacac gccctcgacg tcggtcctca tggtgccctg
     2461 ttttgtcggc acgtcgaccg tccgcttctc gaccagcgcg ccgtccggcg cgagctcctg
     2521 gggcaccccg tcgacgcgca gcagcacccc gcccgcgagg gtctcgagcg cgaccggctt
     2581 gtcgagcttc atcgccgact cccacgcggg cgcgaggtgc tcgacgccct ccacggcgac
     2641 cggcgccggc caggcacccg gcggtgcggc gaccggcggc gcatcgccga ccggctgcgc
     2701 caccgcgacc gcgaccggct cgaccggctt cgcctcaccc gctgcgacca ctggcggcgc
     2761 cgcctcgacc ttcgccgccg ccggaacgac ggtcttgatc accggcgtga ccggcagcgc
     2821 cggatcgtcc ggcgtcgcct cctgcggccg cgggttcgcg gtgcacgcga agctcagcgc
     2881 ggcgaaacaa agcgcgatct ttcgggtctc agcgactgcc gacattgcgc ccaggctatc
     2941 acggcctcgc caccgaccac ctcaccgacc acctcaccga ccaccccgcc gactttgcgc
     3001 ccctgctggt acgccgcgcg gtcgcgacgt acgatgcccg cccgccccca ccagactccg
     3061 cccatgccca cccgccgaag attcctcgag atgacgggcg ccctcggcct gctcgccgcg
     3121 accggctgtg ctcgccgcgg cgcgccgacc accccccccg agcgcgaggc accacggccc
     3181 gtcacgccgc gcagcctgct cgtgctcggc ggcaccggct tcatcggtcc gcacctcgtg
     3241 cgccacgcgg tcgcccgcgg gcatcgggtc acgatcttca cccgcggcaa gcgcgaggcc
     3301 gagctaccgg ccagcgtcga gcgcctgatc ggagatcgca acggcaagct cgaggcgctc
     3361 gccggccggc gctgggacct cgtcgtcgac gactccgcga ccaaccccga ctacgtgcgc
     3421 cagtcgaccg agctgctcgc cgacaaggtc ggtcgctacc tctttacctc gtcgaccggc
     3481 gtctactacc cctacctcga gcccggcgcc gacgagtcgc ggccggtgcg tatgaccgcc
     3541 caggaccccg aggacggcgc cgaggcctac ggcgtcgcca aggcccagtg cgagcgccac
     3601 gtcctcgaca cctttaataa aaatagaaat gacggcggcg tcatcgtgcg tccgacgtac
     3661 atcgtcggtc ctggcgacac caccgaccgc ttcccgtact ggccgcagcg cctgcaacgc
     3721 ggcggcgagg tgctcgcgcc cgggcgccgc gacgaccccg cccaattggt ggacgtccgc
     3781 gacctcgccg aattcatgct caagttgctc gaggacgacc gctcgggaat ttacaacgtc
     3841 gccggtcccg ccgagccgct gacgatctcg caattcctcg agaccgccgt cacgaccctc
     3901 aaccccgacg cgcgcctgac ctggatcgac gactacgagt tcctggccgc gcacgagatc
     3961 ttcgactcga tcccgtgggt gatgcagcgg ggcaacgacg agcactgcat ggagatccgc
     4021 aacgaccggg cgatcgccgc cggcctgaag tttcgtccgc tggcgaccac cctgcgcgac
     4081 accctcgcgt ggtggccgac cgtgccggag gcgcgccgca ccagccccgg cttttcgctc
     4141 aagcccgagc aggaggcgaa ggccctcgcc gactggcacg cccgcgctca gggcagagcg
     4201 ggcgcggccg tctcgaggtt ggcgcagcgg gaacgcggcc cccgccctcg accttacact
     4261 ttttagccgt tgtagcgacc gatcaccacg tcgcgcttgg cgttgctgaa gaacgccgag
     4321 ccggacgagg tgctccagct gtggccgacg atcgggtggg tcgcgtcctt gccgttgacg
     4381 tagaggcgca tcgtgccctg accgacgccc tggtcgtgcg cgccgccggc atcggacatg
     4441 tagcgggtgt cggcggtgcc gtggtagctg gtgctcgtgt cgatgatgtc gacgctaaac
     4501 tgcagggtgc cggcgaccag cggcgcggac gcggtgcggg ccttggggaa gtcggcgacg
     4561 atggcgacgt ggccggtcga ggtgcagctc gagaacgtgc cacaggtcag gttggtgcag
     4621 ccggcgtcga tgtactcgat cgcgatgatg tcgccgggct gcacgtcgtg gatcgactcg
     4681 atgagggtga agccgtcctc gacctcgatc ttgtcgtggt acgaggccgc gtgcgggctg
     4741 gtgcagccga accatgcgac gtagtcgggg tcgtaggcgc gctcgaacag ctgggtgacc
     4801 agcgtggcgc acttcgagcg gttggtgctg taatcgaggc cattgaaccc ctcccaggtg
     4861 atcttggtcg ggtcgccgta gaagttgacc gccggcagca ccgtcgcgac cagcatctcc
     4921 gcgaggtcga ggtgctcgac cacattcgcc tcggccgtct gcggacagac cgcagcaagc
     4981 gtcgcgctca tcatcaccag accacccacc acccgcttac aaacagtcat caccgctccg
     5041 tcctcgcgcc ccattcggcg cgagagctcc tgaccaccga cccgtcgcgc tggccacctg
     5101 gcgcagcgac gacatcccga gcgacctcta tcggatgcga ccctaaagac agtaataagc
     5161 cactgtcaca accccgccat catcgcgccc caccttcaag agccccgcga agcatgc
//
LOCUS       DASWNJ010000454         2277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_8532, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000454 DASWNJ010000000
VERSION     DASWNJ010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8532"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VGB85_07780"
     CDS             complement(<1..240)
                     /locus_tag="VGB85_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133963.1"
                     /translation="MRTSSSSRRDASINRCVVVAWTSSAISTGSDLPANLPTDQLRML
                     YSSSTCRRVGIFASIAASSPVSLHCGIGVPVLSPPV"
     gene            334..888
                     /locus_tag="VGB85_07785"
     CDS             334..888
                     /locus_tag="VGB85_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133964.1"
                     /translation="MQLCAACSRHVFNDAPACPFCGVGLRATSAPATGLIGLALGVAL
                     AGCGARPGDSAEGTDSASSSGGSTSSAGTNTSSGSTTGTGEPTSTGSGSATGSDTGTP
                     TSTSTSTSSTTSTETTEDPSGSETAMDGSTTTTGDTTSFDTTTSTSTTTTDGTSTGDS
                     TTDEWDTIVLPYAGAMPDDGDSPI"
     gene            complement(891..2219)
                     /locus_tag="VGB85_07790"
     CDS             complement(891..2219)
                     /locus_tag="VGB85_07790"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0133965.1"
                     /translation="MRHDAARRRLPIVDLAAPTRTRRVLPLAAGEAPRPRHAVWELTS
                     ACDQKCVHCGPRSGTRRADELTTIECLRLVDELAAIGVGEVTLIGGEAYLRSDLLIII
                     RAIRERGMTATLTTGGYSLDRATAEGMVEAGIQSVSVSIDGLQACHDALRGRPGSFER
                     AFAALRHMRAAGSQISCNSQINARTLQDLEALLELLAPEGIHAWQVQLTLAHGVAADH
                     PEILLQPYQMLEMFAVLDRIVARCETLGVTLYPGNSVGYFGPLEARLRRSSTGRGHYF
                     GCQAGVVGLGIESNGKLKGCPSLGGEANNGGSWREHGLAALWERAPQLAYNRGRGTDS
                     LWGLCADCYYAPVCLGGCTSVSEPLLGRPGNNPMCHHRALELDRQGLRERLEPVAPAP
                     GEPFDHGLLRLIREHKDPELRARLGPVHVDEPRTSRADEPSGAGTSLRPQ"
BASE COUNT          343 a          791 c          859 g          284 t
ORIGIN      
        1 cacgggaggc gagagcacgg gcacgccgat cccgcaatga agtgacaccg gagaggaggc
       61 cgcgatggag gcgaagatcc ccacccgccg gcacgtcgag ctgctgtaca gcatcctcaa
      121 ctggtcggtg ggcaggttcg cgggcaagtc gctgccggtc gagatggccg acgaggtcca
      181 cgcgaccacc acgcatctgt tgatggaagc gtcgcgccgg ctcgacgagg aggtgcgcac
      241 ggacggcgac ggcgcgacct cgacttcggg gtgtaggagc gacagcgccg tcgccggttg
      301 cggagggccg aaccgcgggc gtatgctggc ggcgtgcagc tctgcgccgc gtgctcccgt
      361 catgtgttca acgacgcccc ggcgtgtccc ttctgcggag tcggcctgcg agcgacctcc
      421 gccccggcga ccggcctgat cgggctggcg ctgggggtcg cgctcgcggg ctgcggcgcg
      481 cgtccgggcg acagcgccga gggcaccgac tcggcctcct cgagcggcgg gtcgacgtcg
      541 tccgccggca ccaacacctc gagcgggtcg accaccggca ccggcgaacc gacgagcacc
      601 ggctcgggga gcgccaccgg ctccgatacc ggtacgccga cgagcaccag caccagcacc
      661 tcgagcacga ccagcacgga gacgacggag gacccgagcg gcagcgagac ggcgatggac
      721 gggtcgacga cgacgaccgg cgacacgacc tcgttcgaca cgacgacctc gacgagcacg
      781 acgacgaccg acggcacgag cacgggcgac tcgacgaccg acgagtggga cacgatcgtg
      841 ctgccgtacg cgggcgcgat gccggacgac ggcgactcgc cgatctgagt ttattgcggg
      901 cgcagcgagg tgccggcgcc ggacggctcg tcggcgcgcg aggtcctcgg ctcgtcgacg
      961 tgcaccggcc cgaggcgcgc gcgcagctcg ggatccttgt gctcgcggat cagccgcaag
     1021 agcccgtggt cgaagggctc gccgggcgca ggcgcgaccg gctcgaggcg ctcgcgcaga
     1081 ccctggcggt cgagctcgag cgcgcggtgg tgacacatcg ggttgttgcc ggggcgaccg
     1141 agcaacggct cgctcaccga tgtgcagccg ccgaggcaca ccggcgcgta ataacagtcg
     1201 gcgcacagac cccacagcga gtcggtgccg cgcccgcggt tgtaggcgag ctgcggcgcg
     1261 cgctcccaca gcgcggcgag gccgtgctcg cgccacgagc cgccgttgtt ggcctcgccg
     1321 ccgaggctgg ggcagccctt gagcttgccg ttgctctcga tgccgaggcc gacgacgccg
     1381 gcctggcagc cgaagtagtg gccgcgtccg gtcgagctgc ggcgcagcct ggcctcgagc
     1441 ggcccgaagt agccgacgct gttgccgggg tacagggtga cgccgagggt ctcgcagcga
     1501 gcgacgatgc ggtcgagcac cgcgaacatc tcgagcatct ggtagggctg cagcaggatc
     1561 tccgggtgat cggccgcgac gccgtgggcg agcgtcagct ggacctgcca ggcgtggatg
     1621 ccctcgggcg cgagcagctc gagcagcgcc tcgagatcct gcagcgtgcg cgcgttgatc
     1681 tgcgagttgc acgagatctg cgagccggcg gcccgcatgt ggcgcagcgc cgcgaaggcg
     1741 cgctcgaagc tgccggggcg gccgcgcagg gcgtcgtggc aggcttgcag gccgtcgatc
     1801 gagaccgaca cgctctggat ccccgcctcg accatgccct cggcggtcgc gcggtcgagg
     1861 ctgtagccgc cggtcgtcag cgtcgccgtc atgccccgct cccggatcgc acggatgatt
     1921 attaaaaggt cggagcggag gtaggcctcg ccgccgatca gggtcacctc gccgacgccg
     1981 atcgcggcca gctcgtcgac caaacgcagg cactcgatcg tcgtcagctc gtcggcgcgg
     2041 cgggtgcccg agcgcggccc gcagtggaca cacttctggt cgcaggccga ggtcagctcc
     2101 cacacggcgt gccggggtcg cggcgcctcg ccggccgcca gtggcagcac gcgccgcgtc
     2161 ctcgtcggcg ccgcgaggtc gacgatcggc agacggcggc gagcagcgtc gtggcgcacg
     2221 cggcatcgta acgcgtatgc tccgcggtgt gcaggggagc gcacgcagcg ggatctc
//
LOCUS       DASWNJ010000455         3758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_432480, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000455 DASWNJ010000000
VERSION     DASWNJ010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432480"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1561)
                     /locus_tag="VGB85_07795"
     CDS             complement(89..1561)
                     /locus_tag="VGB85_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0133966.1"
                     /translation="MQRIGVGGFGAVYQAEHVVTRRNFAIKVLLPEFSERPKFAERFV
                     REATTTARIEHENVVDIIDVGRTDQGHLYFAMELLRGETLERTLARDGRLSWQRARTI
                     TLQICDGLRAAHDRGIIHRDLKPANIYRVTRGGNPDFIKILDFGIAKLLAPEDQKAGK
                     GLTSHYEVLGTPLYMSPEQTAADPVDRRSDIYSVGVMLFEMLTGERPYAGESHVELMS
                     KILLGEVPQMAEVAPHIDIPPAFQTIVSKAMARKAAQRYFDMNAMMAALQSLDERGNF
                     TADSGSATAVRSIAAASEDTMLAASSKDEVARTLIRPPAPRSAGPGRPLLAAGLILVG
                     LVTVGAASLLMIGGGPEEQALPPSVERVPLPLTPENRLIVAPDQMPPIPSFIVEAPRT
                     PPRSTRPAPACEAALSSILGSLPAATVRKCIANTGVTSGDRTKVQLAGSAGGKVEVKT
                     LESSGSREFDACLARALGQRHLPAETAPSRCQKALPYRVP"
     gene            1740..2765
                     /locus_tag="VGB85_07800"
     CDS             1740..2765
                     /locus_tag="VGB85_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133967.1"
                     /translation="MTWLSPAPRRLQRSRRVARGRLFRLPDLPDLLDLLVMALSVAQV
                     LAPTSVHAMPMMPTMLAAPAVHAMQAPPPPCPEDPTATPPSEALALKNAAQEMRAAES
                     YVKASRLFRDAVEELPDCATYADERLLWSLWAIETFDKAGVADESGELLHATEDAVTR
                     IEASPNGRLMPDYPRLVAARDRMRDTAPRRPFMRAPRATNPNRAPLATRLGVGLMASG
                     GPLVLAGTIVAGVYSARANRLTDRLAGGGGIYEQWAAAGCGPTAEAGEASACAGLRDA
                     RADIRREGVAVNRVVITSLVLVAIGSAIVLAGLGSYLHGRNTARRGHARVRMVPTFGG
                     LSLSGSF"
     gene            3038..>3758
                     /locus_tag="VGB85_07805"
     CDS             3038..>3758
                     /locus_tag="VGB85_07805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0133968.1"
                     /translation="MTEYKDIARAVFNNLDIIAWATDAQGVTVLSEGKALRAIGFSPG
                     QIVGANVFDMYQDNPVTIAALKRTLAGESHRSVSVEAGRTLETQFTPMFDAAGAVVGI
                     AGLTIDITEQATTRAELERQTEALRRQAEQLAEAELAEARRQETIRAQARAIQELSTP
                     LIPITDEILVMPLIGTMDSMRAKQVLQAILEGAAASRARWVILDITGVAVVDTAVANT
                     ILNAGQAARLLGSEVIVTGIRP"
BASE COUNT          535 a         1434 c         1256 g          533 t
ORIGIN      
        1 actgccggct cgacgggcat cggggccgca ggttaacaca ggcgcccacg aaagcggcag
       61 cgtcaccgca cgccggcccc acgggcgatc acggcacccg gtagggcagc gccttttgac
      121 agcggctcgg ggccgtctcc gcgggcaggt gccgctggcc gagcgcgcgc gccaggcagg
      181 cgtcgaactc ccgcgagccg ctgctctcga gcgtcttgac ctcgaccttg ccgcccgcgc
      241 tgccggcgag ctgcaccttg gtgcggtcgc cgctggtcac ccccgtgttg gcgatgcact
      301 tgcgtaccgt cgccgccggc agcgacccga ggatcgagct cagcgcagcc tcgcacgcgg
      361 gggccggtcg cgtcgacctg ggcggcgtgc gcggggcctc gacgatgaag ctcgggatcg
      421 gcggcatttg atcgggcgcg acgatcagcc ggttctccgg cgtcagcggc agcggcacgc
      481 gctcgacgct gggcggcagc gcctgctcct ccggcccgcc cccgatcatc agcagcgacg
      541 ccgcccccac cgtcacgagc ccgaccagga tcagccccgc cgccagcagc gggcgccccg
      601 ggcccgccga gcgcggcgcc ggcggccgga tcagcgtgcg ggcgacctcg tccttcgagc
      661 tcgccgccag catcgtgtcc tcgctggccg ccgcgatcga ccgcaccgcc gtcgcgctgc
      721 ccgagtccgc ggtgaagttc ccgcgctcgt cgaggctctg cagcgcggcc atcatcgcgt
      781 tcatgtcgaa gtagcgctgc gccgccttgc gcgccatcgc cttgctgacg atcgtctgga
      841 acgccggcgg gatgtcgatg tgcggcgcca cctcggccat ctgcgggacc tcgccgagca
      901 agatcttgct catcagctcg acgtgcgact cgccggcgta gggccgctcg ccggtcagca
      961 tctcgaacag catcacgccg accgagtaga tgtcgctgcg ccggtcgacc gggtcggccg
     1021 ccgtctgctc gggcgacatg tacagcggcg tgccgagcac ctcgtagtgg ctcgtcaggc
     1081 ccttgcccgc cttctggtcc tccggcgcca gcagcttggc gatcccgaag tcgaggatct
     1141 tgatgaagtc ggggttcccg ccgcgcgtga cccggtagat attcgccggc ttgaggtcgc
     1201 ggtggatgat cccgcgatcg tgcgccgcgc gcagcccgtc gcagatctgc aaggtgatcg
     1261 tgcgggcccg ctgccacgac agccgcccgt cgcgcgcgag cgtccgctcg agcgtctcgc
     1321 cgcgcagcag ctccatcgca aaatacaggt ggccctggtc cgtgcgaccg acgtcgatga
     1381 tatcgacgac gttctcgtgc tcgatgcgcg ccgtcgtcgt cgcctcgcgg acgaagcgct
     1441 cggcgaactt cggccgctcg ctgaattcgg gcagcagcac cttgatggcg aagtttcgcc
     1501 gggtgaccac gtgctccgcc tggtacaccg cgccgaagcc gccgacgccg atgcgctgca
     1561 tgacccggta cctcgacccc agcacgacgc cgaccagatc actcgatggc acagggagcg
     1621 tcacggcgct cgccgagcat acacgaggcc cacgaccccc ggcaggcgcg cctttgcgcg
     1681 gcacgaagcg agcggcgcag cgtgcaccgc cgctgcaaca cggttatggt tgcgccgcca
     1741 tgacctggct ttcaccggcg ccgcgacgcc tccagcgcag ccgccgcgtc gcccgtggcc
     1801 gtctattccg cctgcccgac ctgcccgacc tgctcgacct gctggtcatg gcgttgtcgg
     1861 tcgcgcaggt gctcgcgccg acctccgtgc acgcgatgcc gatgatgccg acgatgctgg
     1921 cagcgccggc cgtgcacgcg atgcaggccc cgccgccgcc ctgccccgag gacccgaccg
     1981 ccacgccgcc gagcgaggcg ctcgcgctca agaacgcggc ccaggagatg cgggccgccg
     2041 agtcgtacgt caaggcctcg cggctgttcc gcgacgccgt cgaggagctg cccgactgcg
     2101 cgacctacgc ggacgagcgc ctgctgtgga gcctgtgggc gatcgagact tttgacaagg
     2161 ccggcgtcgc cgacgagagc ggcgagctgc tgcacgccac cgaggacgca gtcaccagga
     2221 tcgaggcgag cccgaacggc cgcctgatgc ccgactaccc gcgcctcgtc gccgcccgcg
     2281 accgcatgcg ggacaccgcc ccgcggcggc ccttcatgcg tgccccgcgg gcgaccaacc
     2341 ccaaccgcgc ccccctggcg acccgcctcg gcgttggttt gatggcgagc ggcggcccgc
     2401 tggtgctcgc tggcacgatc gtcgccgggg tctacagcgc ccgcgccaat cgcctgaccg
     2461 accggctcgc cggcggcggc ggcatctacg agcaatgggc cgccgccggc tgcgggccga
     2521 cggccgaagc cggtgaggcg agcgcgtgcg ccggcctgcg cgacgcccgg gccgacatcc
     2581 gccgcgaggg cgtcgccgtc aatcgcgtcg tcatcacctc gctcgtgctc gtcgccatcg
     2641 gctcggcgat cgtgctcgcc ggcctcggca gctacctcca cggccgcaac accgcccgcc
     2701 gcggtcacgc ccgggtgcgc atggtaccga cattcggcgg cctctcgctg tccggctcgt
     2761 tctgacgccc gcccgggctc tgccccgggc tttcacctgc ggagaagtcc tagccccgcg
     2821 tccggccctt cgtcccgcgc gggcatcctc gcgcatattc accggcaaac cggccaagac
     2881 cggcgacaga tatgcacata gtcgcggccg cgaatgtgcc gtcctcgcga cacgaaaata
     2941 cggcttgcct atccggcccg tcacatacac cgtgatcgga accctcggta tgacgagggc
     3001 gccgggtcat cggtgcgtca cgaacgggag ctggtgaatg actgaatata aggatattgc
     3061 ccgcgcagtc ttcaacaacc tcgatatcat cgcctgggcg accgacgccc agggggtcac
     3121 cgtgctgtcg gagggcaagg cgttgcgagc gatcggattc tcacccgggc agatcgtcgg
     3181 cgccaacgtc ttcgatatgt accaggataa tccggtcacc atcgcggcgc tcaagcggac
     3241 cctcgccggc gagtcgcacc gctcggtcag cgtcgaggcc gggcgcaccc tggagaccca
     3301 gttcacgccg atgttcgacg ccgcgggcgc ggtcgtcggc atcgccgggc tcacgatcga
     3361 catcaccgag caggcgacca cccgggccga gctcgagcgt cagaccgagg cgctgcgccg
     3421 ccaggccgag cagctcgccg aggccgagct cgccgaggcc cgccgccagg aaacgatccg
     3481 cgcccaggcg cgggcgatcc aggagctctc gaccccgctg atcccgatca ccgacgagat
     3541 cctcgtcatg cccctgatcg gcaccatgga ctcgatgcgg gccaagcagg tgctgcaggc
     3601 gatcctcgag ggcgcggccg cctcgcgtgc ccgctgggtg atcctcgaca tcaccggcgt
     3661 cgccgtcgtc gacaccgccg tcgccaacac gatcctcaac gccggtcagg ccgcccgcct
     3721 gctcggcagc gaggtcatcg tcaccggcat ccgcccgg
//
LOCUS       DASWNJ010000456         2391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_149175, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000456 DASWNJ010000000
VERSION     DASWNJ010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149175"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2391)
                     /locus_tag="VGB85_07810"
     CDS             complement(<1..>2391)
                     /locus_tag="VGB85_07810"
                     /inference="COORDINATES: protein motif:HMM:NF017539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEY0133969.1"
                     /translation="GPAGTGKTFLLREVARRISERDATVTPLVVELRGLDRAQDVYEL
                     VSGEFARRKQTLPARAFERDLQDGRIVLLFDGFDELAIRVRSAAIPEHFARILGAARG
                     RARVIVASRAEHFVSHRDAVETLMSRSVTHSAGLAGQLQAITQRRLVATRKFTHEDIE
                     LYLARSLGSAEAGAARMERFRRVHDLPGLAATPRMLAFLVRLTDEQLDQAAARRDAIT
                     SAELYRLVIVDHWLAQQEERFNPPGSAPGPTREALLESATRLALHMWRSTAKSIAATD
                     LDAHTGQQLRRLCEDDSDAARQTLQGRTLLTHGERGQFEFVHQTVMEWLVAQALAIDL
                     REGGTSVDLEHGRLGVFMVDVLRELLGDEAVVAWATRVLTGPVPGNRLAENARIALAS
                     MKREVQVQGADLSGQDLRGQSLAGQDLRGALFDGADLRDCDLSGRDLRGASMQGSKLE
                     TASLRGADLTGTSLKNAVLLYADLSDANLDGADLSGADLSFARLHRARISDVCLTGAV
                     LLGTSFLGVRGPLPVEVEASVEVGGDRVLKEPSGPSACTVVAGSPQGWVVASGHKSGV
                     VTLWAIGRDKLIRVLRVGEAQVVSLAWSPDCRVLAVAVPRKLMLYRTGGGEPEVRLDA
                     NVRSLAFSPDGQRLAGCGEDGRISLWTMADAGPPQKLQGGERIATSVAWSPDGKMLVS
                     GSFDRKLRLWRLDEPGPPIELDGHEGAVVAVAWSPDGTTIASASYDRTVRLWQASDGK
                     LQRVLAGHESAVMGMAWSPIGTAIVTHSAREIRVWGEPWGESRKFATDGALISGVT"
BASE COUNT          348 a          942 c          763 g          338 t
ORIGIN      
        1 acgtgacacc gctgatcagg gcgccgtccg tggcgaactt ccgtgattcg ccccacggct
       61 cgccccatac ccgtatctcc cgcgccgagt gcgtgacgat cgcggtgccg atcgggctcc
      121 acgccatccc catgaccgcc gactcgtgcc ccgccagcac ccgctgcagc ttgccgtcgc
      181 tggcctgcca cagccgcacc gtgcgatcgt aggacgcgct ggcaatggtc gtaccatccg
      241 ggctccacgc caccgcgacc accgccccct cgtgcccgtc gagctcgatc ggcggcccgg
      301 gctcgtcgag ccgccagagc cgaagcttgc ggtcgaacga gccgctgacg agcatcttcc
      361 cgtcggggct ccacgccacg ctcgtggcga tgcgctcacc tccctgaagc ttctgcggcg
      421 gacccgcgtc ggccatcgtc cacagggaga tcctcccgtc ctcaccgcaa cccgcgagcc
      481 gctgtccatc ggggctaaag gccaaactac gcacattggc gtcgagcctg acctccggct
      541 cgccgccgcc ggttcgatac agcatcaact tgcgcggcac cgcgaccgcc agcaccctgc
      601 agtccggact ccacgccagg ctcaccacct gcgcctcgcc gacgcgcagc acgcggatca
      661 gcttgtcacg cccgatggcc cacagcgtca cgacgccact tttgtgccca cttgcgacga
      721 cccagccttg tgggctgccg gcgacgaccg tacacgccga cgggcccgac ggctccttga
      781 gcacccggtc gcccccgacc tccacgctcg cctcgacctc cactggcagc ggcccacgaa
      841 caccgaggaa gctggtcccg agcagcacgg cgccggtcaa acacacgtcc gagatcctgg
      901 cgcgatggag gcgggcgaat gagagatcgg cccccgagag atcggccccg tcgaggttcg
      961 cgtcgctgag atccgcgtac agcaggacgg cgtttttgag cgaggtgccg gtcaagtcgg
     1021 ccccgcgcag gctcgccgtc tccaacttgc tgccttgcat cgacgccccg cgcaggtctc
     1081 gccccgagag atcgcagtcg cgcagatctg cgccatcgaa cagcgccccg cgcaggtcct
     1141 gaccggccag cgactgcccg cgtagatcct gcccgctcag gtcggcgccc tgcacctgca
     1201 cctcgcgctt catgctcgcc agcgcgatgc gggcgttctc cgcgaggcga ttgcccggca
     1261 ccggcccggt cagcacccgc gtcgcccagg cgaccacggc ctcgtcgccg agcagctcgc
     1321 gcagcacgtc gaccatgaac acgccgagtc gcccgtgctc gagatcgacg ctcgtccctc
     1381 cctcacgcag gtcgatcgcc agcgcctgcg cgaccagcca ctccatcacc gtctggtgca
     1441 cgaactcgaa ttgtccgcgc tcgccgtgcg tcagcaaggt ccggccctgc aaggtctggc
     1501 gcgcggcgtc gctgtcgtcc tcgcacaggc gtcgcagctg ctggccggtg tgcgcgtcga
     1561 ggtcggtcgc cgcgatgctc ttggcggtcg atcgccacat gtgcaacgcg aggcgcgtcg
     1621 ccgactcgag cagcgcctcg cgcgtcggtc cgggcgcgct gcccggcggg ttgaagcgct
     1681 cctcctgctg cgccagccaa tgatcgacga tcaccaggcg atacagctcc gccgacgtga
     1741 tcgcgtcgcg ccgcgccgcc gcctggtcga gctgctcgtc ggtcagccgg accaggaacg
     1801 cgagcatccg cggcgtcgcg gccagcccgg gcaggtcgtg cacccggcga aagcgctcca
     1861 tccgcgccgc gccggcctcg gcgctgccga gcgagcgcgc gaggtacagc tcgatgtcct
     1921 cgtgggtgaa cttgcgagtc gctaccagcc gccgctgggt aatcgcctgg agctgccccg
     1981 cgagcccggc ggagtgggtc acggaccgcg acatcagcgt ctcgacggcg tcgcggtgcg
     2041 acacgaagtg ctcggcgcgg ctggccacga tcacccgggc ccggccgcgg gccgccccga
     2101 ggatccgcgc gaagtgctcg gggatcgccg ccgagcggac ccggatcgcc agctcgtcga
     2161 agccatcgaa caacagcacg atacgaccgt cctgcaggtc gcgctcgaac gccctcgccg
     2221 gcagggtctg cttgcgccgg gcgaactcgc cgctgaccag ctcgtacacg tcctgcgcgc
     2281 gatcgaggcc gcgcagctcg accaccagcg gcgtgacggt cgcgtcgcgc tcgctgatcc
     2341 gccgcgcgac ctctcgcagc aggaacgtct tgccggtgcc ggccgggccg a
//
LOCUS       DASWNJ010000457         3838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_113900, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000457 DASWNJ010000000
VERSION     DASWNJ010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113900"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..891
                     /locus_tag="VGB85_07815"
     CDS             64..891
                     /locus_tag="VGB85_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133970.1"
                     /translation="MSNIDRFLDFVRKQWSRLPDDVKEEQATYPRRLGAEYQALTSSL
                     GMPCPPELVLLGDELADVSVFCGLWELAGPEGAVATREEFQERAVQSPCLRPRADMLP
                     LFGYDGDWLLLDRHGAIWLWSHEIPTDDVGCVARSLDELLGRLLDGPEIEDQHALGSG
                     FFWVDGIEYQYFVVHSCASDTSSKKGCCETCVYFDLAPHAIRPFGKHHLTFDFPEPPL
                     ERPRTEPIGLVSTPRGVVDLRQVEVLTALLKIGRARGWTGRTGPLEMGDATPYLDEI"
     gene            complement(1071..1928)
                     /locus_tag="VGB85_07820"
     CDS             complement(1071..1928)
                     /locus_tag="VGB85_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0133971.1"
                     /translation="MQIFSVEGNTQRLDGGAMFGNCPRAVWAGWAPPDDHNRIRLACR
                     SMLVREDSGRHVLLETGIGAFFEPKMRDRFGVQEPEHVLLRSLAALDVKPEDIDVIVL
                     SHLHFDHAGGLLTAWSPEQAPQLVFPNARHVVSAEAWERALRPHARDRASFIPGLTDM
                     LLGTGRLEQVSGPHSDTLGAAYSFTYSNGHTPGLMLTRVAGPAGPVTFLGDLVPGVPW
                     VHLPITMGYDRFPELLIDEKEALLATIVAESGWAFYTHDPEVAASRVTRSAQGRFSAA
                     ESLHDVHWS"
     gene            complement(1959..2654)
                     /locus_tag="VGB85_07825"
     CDS             complement(1959..2654)
                     /locus_tag="VGB85_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133972.1"
                     /translation="MSLPSLPSRAVPATRLLPRQFAYHVELTPAEVLGRIAADADVQV
                     IAGFPARGRSDRKFLAELGRTSFRVCQNLAAGSVDTGPADLRRLALEADLVPTPRGTL
                     VRARFARGPLSKQASFYIMWAASFMWLGFTGITGAKLGIVGAFLALTVPAFVYDLMRA
                     TGSDDDRLQLLNLMEHLLGPAALGDNPEENMPYRHGRRLPELVPPLGHGHAHDDHADD
                     DDPDDDDDVRAPG"
     gene            complement(2784..3692)
                     /locus_tag="VGB85_07830"
     CDS             complement(2784..3692)
                     /locus_tag="VGB85_07830"
                     /inference="COORDINATES: protein motif:HMM:NF013586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEY0133973.1"
                     /translation="MRRLLPLGLLLACEPAASNDGAGHVPRNAAADPVPAATHDGPVS
                     PNDSAARPHDIADRVPELPNDSPARPHDIADRVPEPPNDSPASPNNVLTPPARPVPGD
                     SPRAPPDGFVDLRVVLPTACFAPGYAAPDNFTGAPLPGYAAPGAWMLAGPAAALARAQ
                     ASLATAGLVLLIFDAYRPRRASEAMVAWAERTDQAALVRDGYIARRSGHNHGHTIDLG
                     LARRGDCEPAGWLDMGTPWDTLDERSHTLRATGPARENRRTLLRAMRGAGFRPYPREW
                     WHFGYPLAGTRPRDVPYGAREPDETP"
BASE COUNT          512 a         1209 c         1555 g          562 t
ORIGIN      
        1 gtcggcagct tctcgcctta ggggagcccc accgccgcgg gaatgagtga tacacacgcg
       61 cgcatgtcga acatcgaccg tttcctcgac ttcgtgcgca agcaatggtc caggctaccg
      121 gatgatgtca aggaggagca agcgacctac ccccgccggc tcggcgcgga ataccaggcg
      181 ttgacgtcga gcctgggcat gccctgtccg cccgagctcg tcctgctcgg cgatgagctc
      241 gcggacgtga gcgtcttctg cggcctctgg gagctcgccg ggccggaagg cgcagtcgcc
      301 acgcgcgaag agtttcagga gcgggcggtc caatcgccgt gcctgcgacc cagggccgac
      361 atgctgccgc tgttcggcta cgatggtgat tggctgctgc tcgatcgcca cggtgccatc
      421 tggctttgga gccacgagat cccgacagac gacgtgggct gcgtggcgcg ctcgctcgac
      481 gagctgctcg gcaggctgct ggatggacca gagattgaag accagcacgc gctcggctcg
      541 ggctttttct gggtcgacgg catcgaatac caatatttcg tcgtacactc ctgcgcgtcc
      601 gacacttcgt cgaagaaggg ttgctgcgag acctgcgtgt atttcgatct cgcgccgcac
      661 gcgattcggc cgtttggcaa gcaccacctg acgttcgact ttcccgagcc gccgctcgag
      721 cggccgcgca cggagcccat cggtctcgtc agcacaccgc gtggtgtcgt cgaccttcgg
      781 caagtggagg tgctgaccgc tctcctcaag atcgggcgag cgcgcggctg gaccggacgc
      841 accggtccac tcgaaatggg cgatgcaacg ccgtatctcg acgagatcta gcttcgcggg
      901 cagggcgtga gcggagagcg tggggcgagg cagccgctgg tctgcgcctc gcatgatgtc
      961 gaggagccgg acgctggtgc gttcgactgc aaagcgggcc agcgccccgc gggacgtgtc
     1021 ctgatgcagg ccgtatccga tgcgcgcgcg atggcgcgcc ggcccggggt tcagctccag
     1081 tgcacgtcgt gcagggattc ggcggcggag aagcgcccct gggccgagcg ggtgacgcgg
     1141 ctggcggcga cctcggggtc gtgggtgtag aaggcccagc cggattcggc gacgatcgtg
     1201 gcgagcaggg cctctttctc gtcgatgagg agctcgggga agcggtcgta gcccatcgtg
     1261 atcgggaggt gcacccaggg gacgccgggg acgaggtcgc cgaggaaggt gaccgggccc
     1321 gcggggccgg cgacgcgggt cagcatcagg ccgggggtgt ggccgttcga gtaggtgaag
     1381 ctgtaggcgg cgccgagcgt gtcgctgtgc gggccgctga cctgctcgag acgacctgtc
     1441 ccgaggagca tgtccgtcag gccagggatg aacgaggcgc ggtcgcgggc gtggggcctc
     1501 agggcgcgct cccaggcctc ggcgctgacg acgtggcggg cgttcgggaa caccagctgc
     1561 ggggcctgtt ctggcgacca ggcggtcagc aggccgccgg cgtggtcgaa gtgcaggtgc
     1621 gagagcacga tgacgtcgat gtcctcgggt ttgacgtcga gggcggcgag ggagcgcagg
     1681 agcacgtgct cgggctcctg gacgccgaag cggtcgcgca tcttgggctc gaagaaggcg
     1741 ccgatgccgg tctctagcag gacgtggcgg ccgctgtcct cgcgcacgag catgctgcga
     1801 caggcgaggc ggatgcggtt gtggtcgtcc ggcggggccc agccggccca cacggcccgg
     1861 ggacagttgc cgaacatcgc gccgccgtcc aggcgctggg tgttgccctc gaccgagaag
     1921 atctgcacgc cccgggatag cacgctggcg ggcccgggtc agccgggggc gcggacgtcg
     1981 tcgtcgtcgt ccgggtcgtc gtcgtcggcg tggtcgtcat gcgcgtggcc gtggccgagc
     2041 gggggcacga gctcggggag ccggcggccg tggcggtagg gcatgttctc ctccgggttg
     2101 tcgccgagcg ccgcggggcc gagcaggtgc tccatcaggt tgagcagctg caggcggtcg
     2161 tcgtcgctgc cggtcgcgcg catgaggtcg tagacgaagg cgggcacggt cagcgcgagg
     2221 aaggcgccga cgatgccgag cttggcgccg gtgatgccgg tgaagccgag ccacataaag
     2281 ctcgcggccc acatgatgta gaagctggcc tgcttcgaca gcggtccgcg ggcgaagcgg
     2341 gcgcgcacga gggtgccgcg cggggtcggc acgaggtcgg cctcgagggc gaggcggcgc
     2401 aggtcggcgg gcccggtgtc gacggacccc gcggcgaggt tttggcacac gcgaaagctg
     2461 gtgcggccga gctcggcgag gaacttgcgg tcgctgcgac cgcgcgcggg gaagccggcg
     2521 atcacctgga cgtcggcgtc ggccgcgatg cgcccgagca cctccgcggg ggtcagctcg
     2581 acgtggtacg cgaactgccg cggcaacagg cgggtcgcag gcacggcgcg ggacggcagg
     2641 ctcggcaggc tcatgggcgg ccccgagtat ccgcgacggg acgccgccgg gccagcgacg
     2701 cgtgcacttt gctcgccgcg ctcgctgcgc gggcgccggg gcggcgaggc ggtcgccgcg
     2761 gtccggcgca cctgtccctc gggtcagggt gtctcgtcgg gctcgcgcgc gccgtagggc
     2821 acgtcgcgtg ggcgcgtgcc cgcgagcgga tagccgaagt gccaccactc gcgggggtag
     2881 ggacgaaagc cggcgccacg catcgcgcgg agcagcgtcc gacgattctc acgcgcgggg
     2941 ccggtggcgc gcagggtgtg gctgcgctcg tcgagggtgt cccacggggt gcccatgtcg
     3001 agccagccgg cgggctcgca gtcgccgcgg cgcgcgaggc cgaggtcgat cgtgtggccg
     3061 tggttgtggc cggaccggcg cgcgatgtaa ccgtcgcgca cgagcgcggc ctggtcggtg
     3121 cgctcggccc acgcgaccat cgcctcgctc gcgcggcgcg ggcggtaggc gtcgaagatc
     3181 agcaggacga ggccggcggt cgcgaggctg gcttgcgcgc gggccagcgc cgccgccggt
     3241 ccggccagca tccacgcgcc gggtgccgcg tagccgggca agggcgcgcc ggtgaagttg
     3301 tcgggcgcgg cgtagccggg cgcgaagcag gcggtcggca gcacgacgcg tagatcgacg
     3361 aagccgtccg gcggggcgcg tgggctgtcc ccggggacag gtcgagcggg cggggtgagc
     3421 acgttattcg ggctcgcggg gctgtcgttc ggtggctcgg ggacacggtc ggcgatgtcg
     3481 tgcgggcgcg cggggctgtc gttcgggagc tcggggacac ggtcggcgat gtcgtgcggg
     3541 cgcgcggcgc tgtcgttcgg ggacaccggg ccgtcgtgcg tggccgcagg gaccgggtcg
     3601 gcggcggcgt tccggggcac gtggccggcg ccgtcgttcg aggctgcggg ctcgcaggcg
     3661 agcagcagac cgagagggag gaggcggcgc atgcgggtcg ccattggata gcgcggttgc
     3721 tcgcggcgcg acagagcttc gcggtgtggc gcggcgcgca gcgctgcggc cgcgcgtgac
     3781 aattcgggtg acactcgcgg gcatctctgg ctccctggtt cgtgatgcgc acgctgtt
//
LOCUS       DASWNJ010000458         2161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_643907, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000458 DASWNJ010000000
VERSION     DASWNJ010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643907"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2141)
                     /locus_tag="VGB85_07835"
     CDS             complement(<1..2141)
                     /locus_tag="VGB85_07835"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0133974.1"
                     /translation="MHRDLKPANIFIVDGEDGPLCKVLDFGIVKLSAAALTRPEGPLT
                     LSGATLGTVCYMSPEQIADAQRVDHRADLWSIGVLAYECLTGRRPFRGESLLGVVHEV
                     CYGVPTPPSRLAEVPPGFDRWFSRATHRDRDRRFTSARELLDALKALTARPASATRDE
                     HRDGHREDHRLDATPSWVSDANQIDIGALKDLTFKAPVVREFLDGATRYFVSGSKGLG
                     KTLLLTYKRALLGEQYQVSTPGRERRQAVQFIPEGRPYLDLMGDLPGVDAAQIDRMSR
                     LDECKRLWSFSFRLSILSYHPTLIGNLDDLDALPRRLRALLEGRPVEPTLVLKELLGL
                     SVRQLSQVLDAMENPLERRLRSLHSGVFIFIDKLDQALRRLPRAAWVHMQAGMIEAAW
                     DLMNANRHVKIYATIRDEAFSGYESDIKSNLYGATSSLRYAKHELRELLEKLTYYYEG
                     LPLYDFVLVDTIYASTGARGESPFDFLYRHTLGRPRDLVILSSEISRHRRGLDARAFI
                     RVVQDTSAGLLVSNIFDEMRVFLEVLSDRDRRARFLALLPHDVLTHDDVAEVWCAFHG
                     VDREYFDRHGRDADDVYHPFRELYECGLLGVLEGDNLQRFRQPHDGVAGSRHELPRSR
                     YYLLHPALRALLDRLAGAGRFRPLRHVLIGHGEAWPAHWNLLVDVQREALRRRDLDAE
                     TSEALQTLLDRLDARLAGGDGLDTVRRELAAT"
BASE COUNT          334 a          644 c          874 g          309 t
ORIGIN      
        1 gtcgccgcga gctcgcggcg cacggtgtcg aggccgtcgc cgccggcgag gcgggcgtcg
       61 aggcggtcga gcagggtctg cagcgcctcg ctggtctcgg cgtcgaggtc gcggcggcgc
      121 agggcctcgc gctggacgtc gacgagcagg ttccagtggg ccggccacgc ctcgccgtga
      181 ccgatcagga cgtggcgcag cgggcgaaac cgtccggcgc cagcgaggcg gtcgagcagg
      241 gcgcgcaggg ccgggtggag caggtagtag cgcgagcgcg ggagctcgtg gcgcgagccg
      301 gcgacgccgt cgtgcggctg gcgaaagcgc tggaggttgt cgccctcgag cacgccgagc
      361 aggccgcact cgtagagctc gcgaaacggg tgatagacgt cgtcggcgtc gcggccgtgg
      421 cggtcgaagt attcgcggtc gacgccatga aaggcgcacc acacctcggc gacgtcgtcg
      481 tgggtgagga catcgtgggg cagcagggcg aggaagcggg cgcgccggtc gcggtcggag
      541 aggacctcga ggaacacgcg catctcatca aagatgttgc tgacgagcag gccggcgctg
      601 gtgtcctgga cgacgcggat gaacgcgcgg gcgtcgaggc cgcgacgatg acgcgagatc
      661 tcggaggaca ggatgacgag gtcgcgcggg cggccgagcg tgtgacgata caggaaatcg
      721 aagggcgact cgccgcgcgc gccggtgctc gcatagatcg tatcgaccaa tacgaagtcg
      781 tagagcggga ggccctcgta ataataggtg agcttctcca gcagctcgcg cagctcgtgc
      841 ttggcgtagc gcaggctcga ggtggcgcca tagagattgg acttgatgtc ggactcgtag
      901 ccggagaagg cctcgtcgcg gatcgtcgcg tagatcttga cgtggcggtt ggcgttcatc
      961 aggtcccacg cggcctcgat catgccggcc tgcatgtgca cccacgcggc ccggggcagg
     1021 cggcgcagcg cctggtcgag cttgtcgatg aagatgaaga cgccgctatg tagcgaccgc
     1081 aggcgccgct cgagcgggtt ctccatcgcg tcgaggacct ggctgagctg gcggacgctg
     1141 agcccgagca gctccttgag cacgagggtc ggctcgaccg ggcgaccctc gagcagcgcg
     1201 cgcaggcgcc ggggcagcgc gtcgaggtcg tcgaggttgc cgatcaaggt cgggtgatac
     1261 gacaggatcg acaggcgaaa gctgaagctc cacagccgct tgcactcgtc gaggcgcgac
     1321 atgcggtcga tctgcgcggc gtcgacgccc gggaggtcgc ccatcaggtc gaggtacggg
     1381 cggccctcgg ggatgaactg caccgcttgc cggcgctcgc ggccgggtgt cgatacttga
     1441 tactgctcgc cgagcagcgc gcgcttgtag gtcagcagca gggtcttgcc gaggcccttg
     1501 ctgccggaca cgaagtagcg ggtcgcgccg tcgaggaact cgcgcacgac gggggccttg
     1561 aaggtcaggt ccttgagcgc gccgatgtcg atctggttgg cgtcggagac ccagctcggg
     1621 gtcgcgtcga ggcgatggtc ttcgcggtgg ccgtcgcggt gttcgtcgcg cgtggcgctg
     1681 gccgggcggg cggtgagcgc cttgagggcg tcgagcagct cgcgcgcgga ggtgaagcgg
     1741 cggtcgcggt cgcggtgggt ggcgcgggaa aaccagcggt cgaagccggg cgggacctcg
     1801 gcgaggcgcg agggcggggt cgggacgccg tagcagacct cgtggacgac gccgagcagc
     1861 gactcgccgc gaaacggtcg gcggccggtg aggcactcgt aggcgagcac gccgatcgac
     1921 cacaggtcgg cgcgatggtc gacgcgctgg gcgtcggcga tctgctcggg gctcatgtag
     1981 cagacggtgc cgagggtcgc gccggagagg gtcagcgggc cctcggggcg cgtcagcgcg
     2041 gcggccgaga gcttgacgat gccaaagtcg aggaccttgc acagcgggcc gtcctcgccg
     2101 tcgacgatga agatgttggc gggcttgagg tcgcggtgca cgatctcgag gcggtgggcc
     2161 t
//
LOCUS       DASWNJ010000459         5774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_149233, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000459 DASWNJ010000000
VERSION     DASWNJ010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5774
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149233"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VGB85_07840"
     CDS             <1..625
                     /locus_tag="VGB85_07840"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0133975.1"
                     /translation="YARAVEIQHRLDPDGLATARTYNNLGRACYADRNFTDARRFHAA
                     ALEIRQRRLGIHHPETATSLNNLGAVARAEGDMKTALELFEQSLAIRERLLGPDHPYV
                     AISVNNIAEIYAAQGREDEAITLHERALKIRRARFGEDHPETARSLHNLGVLLLQRGK
                     TGDLEAARAALTSAGKTRAARLGIDHPETRSTLQQLEALPAAAAKKP"
     gene            695..1381
                     /locus_tag="VGB85_07845"
     CDS             695..1381
                     /locus_tag="VGB85_07845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEY0133976.1"
                     /translation="MARASHAVPVIPPAKPSASEVFIPETRSLATLRRAATDCTACEL
                     YKIGTQVVFGEGPKDARLMLIGEQPGDQEDRSGKPFVGPAGALLDRVLAAVEIPRDEV
                     YVTNAVKHFKWKPSGKRRLHQKPGATEIQACRGWMLSEIALIKPAMIVCLGATAAQAF
                     LGKKFSITASRGEVFETPWAPWWMATYHPSALLRMPEEAGRAEAHANFEADLRRASEA
                     LRAGHVSRAS"
     gene            complement(1555..2931)
                     /locus_tag="VGB85_07850"
     CDS             complement(1555..2931)
                     /locus_tag="VGB85_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEY0133977.1"
                     /translation="MRAERVTRACLFIVTLGAACGGEGATSTTSATTGSTGNTSTTST
                     TGVTTTGDDVDTTTGVPTTSDGATTGVVTGSESSGSTSTSGSTSTGADMTSETSGTST
                     TGVDTTGDGSTSGEGTSTGDGSTSTGDESSSTGDTDTDTGGDLAIYGYLGMSGSNTLV
                     RFETESAMVLPPAKNLLPHASYPYDMEIKPDGSEVWIVGAAGDGVKIADTATGQITHD
                     IDLTGVGKYPVDVVFNVAGTLAYVSARDSKVLVVIDALTYTVKSTIPLGVYDGGKMTF
                     DPCSGRIFMVDWFDKSLLVVEPTLKKVTAIVLGTSLWDLALDPVTRLLYVLDRGAAKV
                     HIFDTDIMQFQSSLAVGKDPWGIDITVDGHLLVVACEDDHTVRFIDTDSLMSATLTLP
                     ADALPRDVAIHPDQLRAYVPTGQIAGEDGVFEIDMLTQSLTATITAGPSNLNSNVVAV
                     RPQAVECP"
     gene            complement(2984..3985)
                     /locus_tag="VGB85_07855"
     CDS             complement(2984..3985)
                     /locus_tag="VGB85_07855"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY0133978.1"
                     /translation="MERRAAILGSGHYVPTKVVTNDDLAQIMPTTHEWIHQRTGIRER
                     RFIEESGIGASDLAIPAVKMAVEQAGRELKDIDAVLFATLSPDHQFPGAGCLLIHKLG
                     MPGVPALDVRNQCSGFLYSLSVADAWIRCGMYRNVLVVGSEVHSTGVEFTERGRDVAV
                     LFGDGAGAVLVGESTRPEQGVRSLELHADGSGAYDLWLEAPASKMMPRLTHEMLEAGK
                     HYPAMNGKQVFRWATDKMPEVALSVLKKAGMSIEEIDMFVPHQANMRINQMVAHKLGI
                     PEHKVVHNIERYGNTTAATIPIGLSESWREGKIGTGSKVLMAAFGAGFTWGGAVAVL"
     gene            complement(4203..4275)
                     /locus_tag="VGB85_07860"
     tRNA            complement(4203..4275)
                     /locus_tag="VGB85_07860"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4240..4242),aa:Ala,seq:ggc)
     gene            4545..5237
                     /locus_tag="VGB85_07865"
     CDS             4545..5237
                     /locus_tag="VGB85_07865"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY0133979.1"
                     /translation="MDLAELFQQGGPLMWVILVASIVGVGVFFERMWSTQRAKVLPRA
                     FVDRIRALVAKGKTNEALLLCEENGSSIALIMAAALRAYEHSTNRSEIKEAVEEVGAR
                     EVAHMDRNVEVVGTVATISPLLGLLGTVVGMIQVFKEFVAAFASGQVGPDTFAQGIWQ
                     ALITTAYGLIVAIPTLLLYRFLQGRTGRIVGEMEEDAMGIVNLLEDSRRGRRADRDRA
                     DRADHPMPEPAR"
     gene            5249..5665
                     /locus_tag="VGB85_07870"
     CDS             5249..5665
                     /locus_tag="VGB85_07870"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY0133980.1"
                     /translation="MGASGLRGRGRAVADGAIVDLTPLIDVTFQLLIFFLLTATFNND
                     AAFKVKLPKATANDPATEVKAIVIEISEDGRFEVDKKVVDESELQTRLCMAAKESQAS
                     TVNIKADQNTKHEKVVQAMDVAKACGIEKLGILHRR"
BASE COUNT          904 a         2118 c         1844 g          908 t
ORIGIN      
        1 ctacgcgcgc gccgtcgaga tccagcaccg cctcgacccc gacggcctcg ccaccgcgcg
       61 cacctacaac aacctcggcc gcgcctgcta cgccgaccgc aacttcacag acgcccgccg
      121 cttccacgcc gccgccctcg agatccgcca gcgtcgcctc ggcatccacc accccgagac
      181 cgcgacgagc ctcaacaacc tcggcgccgt cgcccgcgcc gagggcgaca tgaagaccgc
      241 cctcgagctg ttcgagcagt cgctcgcgat ccgcgagcgc ctgctcggcc ccgaccaccc
      301 ctacgtggcg atcagcgtca acaacatcgc cgagatctac gccgcgcagg gccgcgagga
      361 cgaggcgatc accctgcacg agcgcgccct gaagatccgc cgcgcccgct tcggcgagga
      421 ccaccccgag accgcccgca gcctgcacaa cctcggcgtc ctgctcctcc agcgcggcaa
      481 gaccggcgac ctcgaggccg cccgcgccgc cctcacctcc gccggcaaga ccagggccgc
      541 ccgcctcggc atcgaccacc ccgagacccg cagcaccctg caacagctcg aggccctccc
      601 cgcagccgcc gcgaagaagc cctgatccgg caaaaatcga cgggcacgtt ttcgcgcccg
      661 tgccatgctt gggcgaccag gaggtgtccc ccacatggcc cgtgcatccc acgcagtccc
      721 cgtcattccc ccggcgaagc cctcggcgag cgaggtgttc atcccggaga cccgctcgct
      781 ggcgacgctg cgccgcgccg cgaccgactg caccgcctgc gagctttata aaattggcac
      841 gcaggtggtg ttcggcgagg gccccaagga cgcacgcttg atgctgatcg gcgagcagcc
      901 gggcgaccag gaggaccgca gcggcaagcc cttcgtcggc cccgccggcg ccctgctcga
      961 ccgcgtgctc gcggccgtcg agatcccgcg cgacgaggtc tacgtcacca acgccgtcaa
     1021 acattttaag tggaagccct ccggcaagcg acgcctgcac caaaaacctg gcgccaccga
     1081 gatccaggcc tgccgcggct ggatgctctc ggagatcgca ctaattaaac cggcgatgat
     1141 cgtgtgcctc ggcgcgaccg cggcccaggc gttcctgggg aagaaattca gcatcaccgc
     1201 cagccgcggc gaggtgttcg agaccccgtg ggccccgtgg tggatggcca cctaccaccc
     1261 ctccgcgctg ctgcgcatgc ccgaggaggc cggccgcgcc gaggcccacg cgaacttcga
     1321 ggccgacctg cgccgcgcct ccgaggccct gcgcgccggc cacgtctcac gcgcctcgtg
     1381 agcgctcacg ggcgtgaccc tctttaatcc gtgaccgcag gccacgtgac acctggcgct
     1441 tgtcgagatc ggcctggacg atctccgcaa gctcggggtc cttctcgatt tccgctcgcg
     1501 catacgcgag aaaggtattt ttcgagtttg tcattctgtg cacctttcac ggcttcacgg
     1561 gcactcgacc gcctgcggcc gcaccgcgac cacattgcta ttcagattgc tcggcccggc
     1621 cgtgatcgtc gcggtcagcg actgcgtcag catgtcgatc tcgaacacgc cgtcctcgcc
     1681 ggcgatctgg ccggtcggga cgtacgcgcg cagctggtcc gggtggatcg cgacgtcgcg
     1741 ggggagggcg tcggctggca gcgtcagggt cgcgctcatc agggagtccg tgtcgatgaa
     1801 gcgcaccgta tggtcgtcct cgcaggcgac caccagcagg tgcccgtcga ccgtgatgtc
     1861 gatgccccat gggtccttgc cgaccgcgag gctgctctga aattgcatga tgtcggtgtc
     1921 gaagatgtgg accttcgcgg ccccgcggtc gagcacgtac agcagccggg tcaccgggtc
     1981 gagcgcgagg tcccacagcg aggtcccgag cacgatcgcc gtgacctttt ttaaggtcgg
     2041 ctcgaccacc agcaggctct tgtcgaacca gtcgaccatg aagatccggc cgctgcacgg
     2101 gtcgaaggtc atcttgccgc cgtcataaac cccgagcggg atcgtcgact tcacggtgta
     2161 ggtcagcgcg tcgatcacga cgagcacctt gctgtcgcgc gcgctgacgt aggcgagcgt
     2221 gcccgcgacg ttgaacacga cgtcgaccgg gtacttgccg acgcccgtca ggtcgatgtc
     2281 gtgcgtgatc tggcccgtcg ccgtgtccgc gatcttcacg ccgtcccccg cggcgccgac
     2341 gatccacacc tcgctgccgt cgggcttgat ctccatgtcg taggggtagc tcgcgtgcgg
     2401 cagcaggttc ttggccggcg gcagcaccat cgccgactcg gtctcgaagc gcaccagcgt
     2461 gttgctgccc gacatgccga ggtacccgta gatcgccaaa tcgccgccgg tgtccgtgtc
     2521 ggtgtcaccc gtgctgctgc tctcgtcgcc cgtgctcgtg ctcccgtcgc ccgtgctcgt
     2581 accctcgcca ctcgtgctgc cgtcgccggt cgtgtcgacc cccgtcgtgc tcgtcccgct
     2641 cgtctcgctc gtcatgtcgg cgcccgtgct cgtgctcccg ctcgtgctcg tgctgccgct
     2701 cgactcgctg cccgtcacca cgcccgtggt cgcgccgtcg ctcgtcgtcg gcacgccggt
     2761 ggtcgtgtcg acatcatcgc cggtcgtcgt caccccggtc gtcgaggtcg tgctcgtgtt
     2821 cccggtcgac ccggtcgtcg cggacgtcgt cgacgtcgcg ccctcgccac cgcaggccgc
     2881 accgagggtg acgatgaaca gacaggcccg ggtgacacgt tcggctcgca tgaggccgcc
     2941 aacctagcac ggcggcccga cccggcaatc cgccgcgctt cgcctacagc accgcgaccg
     3001 cgccgcccca cgtgaagccg gcgccgaagg ccgccatcag caccttcgag cccgtgccga
     3061 tcttgccctc gcgccacgac tcactgagcc cgatcgggat cgtcgcggcg gtggtatttc
     3121 cataacgctc gatgttgtgg accaccttgt gctcggggat cccgagcttg tgggcgacca
     3181 tttggttgat gcgcatgttg gcctggtgcg gcacgaacat gtcgatctcc tcgatcgaca
     3241 tgccggcctt cttgagcacc gacagcgcca cctccggcat cttgtcggtc gcccagcgga
     3301 acacctgctt gccgttcatc gccgggtagt gcttgccggc ctcgagcatc tcgtgggtca
     3361 ggcgcggcat catcttgctc gccggggcct cgagccacag gtcgtacgcg ccggagccgt
     3421 cggcgtgcag ctcgagcgag cgcacgccct gctccgggcg ggtcgactcg ccgacgagca
     3481 ccgcgcccgc gccgtcgccg aacagcaccg cgacatcgcg gccgcgctcg gtgaactcga
     3541 cgccggtcga gtggacctcg gagccgacga cgagcacgtt gcgatacatg ccgcagcgga
     3601 tccacgcgtc ggcgaccgac aggctgtaca ggaagcccga gcactggtta cgaacgtcga
     3661 gcgccggcac ccccggcatg ccgagcttgt ggatcagcag gcagcccgcg ccggggaact
     3721 ggtggtccgg gctcagcgtc gcgaacagca ccgcgtcgat gtccttgagc tcgcgcccgg
     3781 cctgctcgac cgccatcttc accgccggga tcgccaggtc actggcaccg atccccgact
     3841 cctcgatgaa gcgacgctcg cggatccccg tgcgctggtg gatccactcg tgcgtggtcg
     3901 gcatgatctg cgcgaggtcg tcgttggtga cgaccttcgt cggtacgtag tggccggagc
     3961 ccagaattgc ggcgcgtctc tccattcatc actaggtatc ggggccgcga cgccacggca
     4021 agatgcgcag gaatatgcgc agcgcgcctc gttcgctgga gaggtccact ccgcgccgct
     4081 gactcacgag ccagaatgac cacgagccga aacgaccccg gaagacctcg accgcccgag
     4141 tgcggcgaaa aacgaagagg cgggcagcct cgcggcgccc gcctcaacgt gtcttcttcg
     4201 agtggaggca agggggatcg aacccctgac cttcgcgctg ccagcgcgat gctctcccag
     4261 ctgagctata cccccatggg acggactgtt tcggccactg cccggaggcc gtggtggggc
     4321 gcgtgtgtaa cagcgccccg gagcggcgtc aagcacgaat ttcgaaggcc gggccgggaa
     4381 agcgacgcgg cccgctggcg cgctcgcctc cgccggcgcc tgcgggtgct ctggccgcgc
     4441 gtgccacggg ggcgacccct tgcgcgggcg ggggcgggcg ccgcatgatt cgcgggcctc
     4501 gaaggggcct tgcggtgcaa ggctcccggc ccgaccccca cgccatggat ctcgcggaac
     4561 tgtttcagca aggcggcccg ctgatgtggg tcattttggt cgcgtcgatc gtcggcgtcg
     4621 gggtgttctt cgagcgcatg tggtcgacgc agcgggccaa agtgctgccg cgcgccttcg
     4681 tcgaccggat cagggcgctg gtcgccaagg gcaagaccaa cgaggcgctg ctgctgtgcg
     4741 aggagaacgg ctcgagcatc gcgctgatca tggccgccgc cctgcgcgcc tacgagcaca
     4801 gcaccaaccg ctccgagatc aaggaggccg tcgaggaggt cggcgcgcgc gaggtggccc
     4861 acatggaccg caacgtcgag gtcgtcggca ccgtcgccac catctcgccg ctgctcggcc
     4921 tgctcggcac cgtcgtcggc atgatccagg tgttcaagga gttcgtcgcg gccttcgcct
     4981 ccggccaggt cggccccgac accttcgcgc agggcatctg gcaggccctg atcaccaccg
     5041 cctacggcct gatcgtcgcc atccccaccc tgctgctgta ccgcttcctc cagggccgca
     5101 ccggacgcat cgtcggcgag atggaggagg acgcgatggg catcgtcaac ctgctcgagg
     5161 acagccgccg cggccgtcgt gccgaccgtg accgcgccga ccgtgccgac catccgatgc
     5221 cggagcccgc gcgatgaact ttcgcggcat gggagcgagc ggtctgcgtg gccgcggccg
     5281 cgccgtcgcg gacggggcga tcgtcgacct gacgccgctg atcgacgtca ccttccagct
     5341 gctgatcttc ttcctgctga ccgccacgtt caacaacgac gccgccttca aggtcaagct
     5401 gcccaaggcg accgccaacg acccggccac cgaggtcaag gcgatcgtca tcgagatctc
     5461 cgaggacggc cgcttcgagg tcgacaagaa ggtcgtcgac gagtccgagc tgcagacccg
     5521 cctgtgcatg gccgccaagg agtcgcaggc ctccaccgtc aacatcaagg ccgaccagaa
     5581 caccaagcac gagaaggtcg tgcaggcgat ggacgtcgcc aaggcctgcg gcatcgagaa
     5641 gctcggcatc ttgcatcgtc gatgacgata atgcagtgat gaaccgatga accgatgatc
     5701 agtaaacgct ggcagaccgg cgcggacctg cggtagaaca gccgcggagt cccccccatg
     5761 tccgtcacgt tcta
//
LOCUS       DASWNJ010000460         1521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_149265, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000460 DASWNJ010000000
VERSION     DASWNJ010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149265"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VGB85_07875"
     CDS             complement(<1..312)
                     /locus_tag="VGB85_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133981.1"
                     /translation="MTIDEPRAYAEVEFTWDKIPRAFDPPTHGAVRIDGYRESEGFAD
                     IFMIDLVLTEGRPATGSVQRAQVFSRLGSMSGRLPLPGERFALCDGWKPPFAVATTLA
                     RG"
     gene            complement(402..>1521)
                     /locus_tag="VGB85_07880"
     CDS             complement(402..>1521)
                     /locus_tag="VGB85_07880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY0133982.1"
                     /translation="IFGRENYTAVGVVYALIGSVIVGYAYRIAERVSKRSWVPPVIGL
                     VMVFYYPLISLGGYTLSEVPFGMFLLMATLALLRLAQDGRPRDAWLAGTWAGIATAFR
                     PQILMSIIIFGLVWVVMHKKMPKVRIKHLTFAAIPIAIVLVFSAVRLHHHTGRWGLVS
                     ENGRFNQLFGRCHNKKAVATPADVSRGRIRFGPPPLIQLEKRAKTAPDSWVQLDPAIG
                     AELSYTGYISDEKALRELMQKCIAKTGILGQLKYATVNILMLSGYNTPWPDSGRDLWR
                     RTANQWQSFHTAFLTFPALLTLFTCFWRRTATRHGILAVHIWAMMVTAAIYFGDTRLR
                     APYDPMLVLLALETYLFVGGTIFERARGWWQRRRSSAT"
BASE COUNT          260 a          505 c          548 g          208 t
ORIGIN      
        1 cccgcgcgcg agggtcgtcg ccaccgcgaa cgggggcttc cagccatcgc acagcgcgaa
       61 gcgctcgcca gggagcggca ggcgcccgct catcgagccg agccgcgaga agacctgcgc
      121 gcgctggacc gaccccgtcg ccggccggcc ctccgtgagc accaggtcga tcatgaagat
      181 gtcggcgaag ccctcggatt cgcggtaccc gtcgatacgg acggcgccat gagtcggggg
      241 atcgaacgcc cgcgggatct tgtcccacgt gaactcgacc tcggcgtaag cgcgtggctc
      301 gtcgatggtc atcgtccgct cacgatcgca gcgtaggcag gcgggcgcgc aggcgtcaag
      361 tcgcggccgc gcgggaaccc ggcgcccgac gcccgcacgc gctacgtcgc gctcgaccgc
      421 cgccgctgcc accacccgcg cgcccgctcg aagatcgtcc ccccgacgaa caaatacgtc
      481 tcgagcgcga gcagcacgag catcgggtca tacggcgcgc gcaggcgggt gtccccgaag
      541 tagatcgcgg cggtgaccat catcgcccag atgtgcacgg cgaggatgcc gtggcgcgtg
      601 gcggtgcgcc gccagaagca ggtgaacagg gtcagcaggg ccgggaaggt caggaaggcg
      661 gtgtggaacg actgccactg gttggcggtg cggcgccaca ggtcgcggcc ggagtcgggc
      721 cacggggtgt tgtagccgga cagcatcagg atgttgaccg tcgcgtactt gagctggccg
      781 aggatgccgg tcttggcgat gcacttctgc atcagctcgc gcagggcctt ctcgtcggag
      841 atgtagccgg tgtacgacag ctcggcgccg atcgcagggt cgagctggac ccacgagtcg
      901 ggggccgtct tggcgcgctt ctcgagctgg atcagcggcg gcggtccgaa gcggatccgg
      961 ccgcgcgaga cgtccgccgg ggtggcgacg gccttcttgt tgtgacagcg gccgaacagc
     1021 tggttgaacc ggccgttctc gctgacgagg ccccagcggc cggtgtggtg gtgcaggcgc
     1081 acggcggaga acacgagcac gatcgcgatc ggaatggcag cgaaggtcag gtgcttgatc
     1141 cgcaccttgg gcatcttctt gtgcatcacg acccacacga ggccgaagat gatgatcgac
     1201 atcaggatct gcggacgaaa cgcggtggcg atgccggccc aggtgccggc gagccaggcg
     1261 tcgcgcgggc ggccgtcctg ggccaggcgc agcagcgcca gcgtcgccat cagcaggaac
     1321 atcccgaacg ggacctcgct gagggtgtaa ccgccgagcg agatcagcgg gtagtagaac
     1381 accatcacca ggccgatgac cggcggcacc cagctgcgct tcgagacccg ctccgcgatc
     1441 cggtacgcgt agccgacgat caccgagccg atcagcgcgt acacgacccc gaccgcggtg
     1501 tagttctcgc gcccgaagat c
//
LOCUS       DASWNJ010000461         1504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_8660, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000461 DASWNJ010000000
VERSION     DASWNJ010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8660"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1214
                     /locus_tag="VGB85_07885"
     CDS             <1..1214
                     /locus_tag="VGB85_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133983.1"
                     /translation="SIVAFANVDTINPIDTHSGQKNGLTSTFASPAITTGTANGVAVW
                     FGSQVWAGATCPASPIVPPTGFTEVQDTCLPSASTAILYDSAYKQLGAAGLQPAFNGS
                     SPYANTNTAQVVALRAAGAPTCTVGDNFSASYTTVGTVASPAIVEPSGLAASRLTPGA
                     IYVHNEDTTAIVAISSTNAATLGTFNVAGVVPADWEDVASGPCPAGKCIYMGDIGRAS
                     ANFPTPPSTFAVYRIPEPNIGAGQTSGDLTAEKFPFQYPDTPKDAETIMVHPTTGDIY
                     VITKSGTGLSKVYKFPTPMPAPNVMSTLVFVANIQLPTNGDTNFASATAGAIHPCADR
                     FLLRTYKRVYEFRAASGAAFETAFAATPVVLTDTVEGQGEAIEYEYNGASYFTMSETP
                     SPFKLKRVARI"
     gene            complement(1246..>1504)
                     /locus_tag="VGB85_07890"
     CDS             complement(1246..>1504)
                     /locus_tag="VGB85_07890"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0133984.1"
                     /translation="RIAADQARAVIFDLTGAAFIDVETAEALHRALRVLRLQGCTVVV
                     AGVLPALARLLVASDVRLDVVVHQTIADALRVLMRAPGAST"
BASE COUNT          258 a          572 c          477 g          197 t
ORIGIN      
        1 gcagcatcgt cgcgttcgcc aacgtcgaca cgatcaaccc gatcgacacc cacagcgggc
       61 aaaagaacgg cctgacctcg accttcgcca gccccgcgat caccaccggc accgccaacg
      121 gcgtcgcggt gtggttcggc tcgcaggtgt gggccggcgc cacctgcccg gcgagcccga
      181 tcgtgccgcc gaccggcttc accgaggtgc aagacacctg cctgccctcg gcgtcgaccg
      241 cgatcctgta cgattcggcg tacaagcagc tcggcgccgc cggcttgcag cccgccttca
      301 acggcagctc gccctacgcg aacaccaaca ccgcgcaggt ggtcgcgctg cgggccgcgg
      361 gcgcgccgac ctgcacggtc ggggacaact tctccgccag ctacacgacc gtcggcacgg
      421 tcgcgtcccc ggcgatcgtc gagccctcgg gcctcgccgc cagccgcctg acccccggcg
      481 cgatctacgt gcataacgag gataccaccg cgatcgtggc gatcagcagc accaacgcgg
      541 ccaccctcgg gaccttcaac gtcgccggcg tcgtcccggc cgactgggag gacgtcgcga
      601 gcggtccgtg cccggcgggc aagtgcatct atatgggcga catcggccgc gcgagcgcca
      661 acttcccgac cccgccgtcg accttcgcgg tctaccggat ccccgagccc aacatcggcg
      721 ccggccagac cagcggcgac ctgacggccg agaagttccc gttccagtac cccgacacgc
      781 ccaaggacgc cgagacgatc atggtgcacc cgaccaccgg ggacatctat gtgatcacca
      841 agtcgggcac gggcctcagc aaggtctata aattcccgac gccgatgccg gcgccgaatg
      901 tcatgtcgac gctggtgttc gtcgccaata tacagctgcc gaccaacggc gacaccaact
      961 tcgcctcggc gaccgccggc gcgatccacc cgtgcgccga tcgtttcctg ctgcgcacct
     1021 acaagcgcgt ctacgagttc cgcgccgcct ccggcgcggc cttcgagacc gccttcgccg
     1081 cgacgccggt ggtcctgacc gacacggtcg aggggcaggg cgaggcgatc gagtacgagt
     1141 ataacggcgc cagctacttc acgatgagcg agaccccctc gcccttcaag ctcaagcgcg
     1201 tcgcccggat ctgaaaccgg tcgcggtgtc accggcgcga gcgactcagg tcgacgcgcc
     1261 gggcgcccgc atgagcacac gcagcgcgtc ggcgatcgtc tgatgcacga caacgtcgag
     1321 ccggacgtcg ctggcgacca gcagcctcgc cagcgccggc aagacgccgg cgacgacgac
     1381 ggtgcagccc tggagcctga gcacacgcag ggcccgatgc agcgcctcgg cggtctcgac
     1441 gtcgatgaag gcggcgccgg tgaggtcgaa gatgacggcg cgcgcctggt cggcggcgat
     1501 ccgc
//
LOCUS       DASWNJ010000462         4442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_8688, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000462 DASWNJ010000000
VERSION     DASWNJ010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8688"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2495)
                     /gene="polA"
                     /locus_tag="VGB85_07895"
     CDS             complement(<1..2495)
                     /gene="polA"
                     /locus_tag="VGB85_07895"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEY0133985.1"
                     /translation="MAKAERLVLVDGSWLVYRAFFAIPANLQTKTGLPTNAIFGFATM
                     FRKLFSGRMPDRGAVVFDSPAATHRELKFPAYKAQRPSMPGELAQQLSTIDRLVAANH
                     FPMIRRPGYEADDIIATLARRGVEAGLEVVIVAGDKDLAQMVGDHVRMQDTMRDVTYD
                     AELVRKKWGVAPGLIPDLLALMGDDVDNIPGVPGIGQKGAAGLLEKYGSLDGILAHLD
                     DLKGKQKAALVEHTEAVKLYRQLATVDVNAELDVTLDDLRLTPPDPKQLDALYRELEF
                     YSLLTAGGDARQGDAPDIEVEVPTTIEAARAAVDIIATTAALPPGQGVAVTPIYEPPT
                     RVHHAHLVGVALATGGRRPLYLAFRGAGAPLGDPAIDLLRAVLADEARPKFVHDVKQL
                     AILCARHGLSLGGEIVDTMITSFLVDPVKCIPHALDQVAREYVQRSIPAAKSLLGAGQ
                     STRAPGVVDIAAAAKYAAQLADVILALAPVLQKRLAEAGQVEHYKNLEHPLAYVLARM
                     ELAGIRVDREDLTRMGEEFHARKAEIEKRIYALAGREFNIGSTKQLADVLFDELKLPV
                     IKKNKTGYSTDAEVLERLAPKHEIAQQVLAQRELAKLINTYTDVLREAIAPETGRIHA
                     TLQQTVGVSGRLISTDPDLQRTPIRTPEGRRIRRAFVAPEGMRLVSADWSQIELRVLA
                     HFSKDPRLVEAFRDKLDLHRRTASLLFHVEPEAVTKEQRNVGKTVNFATIYGQGATAL
                     GQILGVPRKDADSYIEKYFEYYADVRRWLDRTIAQAHEDGFVTTLLGRRRYIPELSMR
                     NNSDRAAGERIAANTPIQGSAADLCKLAMLQIDR"
     gene            complement(2589..3308)
                     /locus_tag="VGB85_07900"
     CDS             complement(2589..3308)
                     /locus_tag="VGB85_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133986.1"
                     /translation="MTHAHDTSPRSLRIVAAYSALAGLCPLIPLPYVDDFLIRRVTRR
                     MHSALYDAHGLFLSTSGERVLTEAPSVWLRGAATSVALFPVRRLLRKVVYLLAIRDCA
                     DVAAGVFHDGWLLARLLEQDNKGGKPGPSLEDTRYLQKVRKAMLRTYKEVDPAPFKRA
                     LLGAFLGARVGVEHAVSAIQRRRGGEAMPNVEDVEPVVDRMRAAAMSQWKYLDSLEQQ
                     FRRHLGLPAEQAGIEAQATAS"
     gene            3590..>4442
                     /locus_tag="VGB85_07905"
     CDS             3590..>4442
                     /locus_tag="VGB85_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0133987.1"
                     /translation="MTATNYGVLGQLEVPLGLGLLRLSTEGRPAESDAIDVIHFALDQ
                     GIRVLDTADSYALDDRDMHYGERLVRKAIAAWEGPREQVRVITKAGLARPKGRWVPNA
                     RPDHLRKMVEGSLTALGVERIFLLLLHANDPKTPYEDSLGALAQLQKEGKIEHLGLSN
                     TSIAEVRQAERHFAVCAIQNELSVIDRNSGVEGLVALARRMNIPFLAHRPLGGHAKIG
                     NLLKNRAVKPIAARHKITPHEAALAALLDLGAPVIPLFGATKRASVESSLRALKVPFD
                     ERDRAELG"
BASE COUNT          648 a         1432 c         1680 g          682 t
ORIGIN      
        1 cggtcgatct gcagcatcgc cagcttgcac aggtcggccg cggacccctg gatcggcgtg
       61 ttggcggcga tgcgctcgcc cgcggcgcgg tcgctgttgt tgcgcatcga cagctccggg
      121 atgtagcggc ggcggccgag cagggtcgtg acgaagccgt cctcgtgggc ctgggcgatc
      181 gtgcggtcga gccagcggcg gacgtcggcg tagtattcga agtatttttc aatatagctg
      241 tcggcgtcct tgcgcgggac gccgaggatc tggccgagag cggtcgcgcc ttgcccgtag
      301 atcgtcgcga agttgacggt cttgccgacg ttgcgctgct ccttggtgac cgcctcgggc
      361 tcgacgtgga acagcaggct ggcggtgcgg cggtgcaggt cgagcttgtc gcggaaggcc
      421 tcgacgaggc gcgggtcctt gctaaagtgg gccagcacgc gcagctcgat ctgactccag
      481 tcggcggaga ccaggcgcat gccctcgggc gcgacgaagg cgcggcggat ccggcggccc
      541 tcgggggtgc ggatcggcgt gcgttgcagg tcggggtcgg tggagatcag gcggccggac
      601 acgccgacgg tctgctgtaa ggtcgcgtgg atcctgccgg tctcgggcgc gatcgcctcg
      661 cgcagcacgt ccgtataggt gttgatcagc ttggcgagct cgcgctgcgc gagcacctgc
      721 tgggcgatct cgtgcttggg ggcgaggcgc tcgagcacct cggcgtcggt cgagtagccg
      781 gtcttgttct tcttgatgac ggggagcttg agctcgtcga acaacacgtc ggcgagctgc
      841 ttggtcgacc cgatgttgaa ctcgcggccg gcgagcgcat agatccgctt ctcgatctcg
      901 gccttgcggg cgtggaactc ctcgcccatg cgcgtgaggt cctcgcggtc gacgcggatc
      961 cccgcgagct ccatgcgcgc gagcacgtag gcgagcgggt gctcgagatt tttataatgc
     1021 tcgacctggc cggcctcggc gagccgcttt tgcagcaccg gcgcgagcgc gaggatcacg
     1081 tcggcgagct gggcggcgta cttggccgcg gcggcgatgt cgaccacgcc gggcgcgcgc
     1141 gtcgactgtc ccgcgccgag cagggacttg gccgcgggga tcgatcgttg cacgtactcg
     1201 cgggcgacct gatcgagcgc gtgcgggatg cacttgaccg ggtcgacgag gaacgaggtg
     1261 atcatcgtgt cgacgatctc gccgccgagc gagagcccat gacgcgcgca caggatcgcc
     1321 agctgcttga cgtcgtggac gaatttcggg cgcgcctcgt cggcgagcac cgcccgcagt
     1381 agatcgatcg cggggtcgcc gagcggcgca ccggcgccgc gaaacgcgag ataaagcggc
     1441 ctgcgtccgc cggtcgccag cgcgacgccg acgaggtgcg cgtggtggac ccgcgtcggc
     1501 ggctcgtaga tcggcgtgac ggcgacgccc tgaccgggcg gcaatgcggc ggtggtggcg
     1561 atgatgtcga ccgcggcgcg agcggcctcg atcgtcgtcg ggacctcgac ctcgatgtcg
     1621 ggcgcgtcgc cctggcgcgc gtccccgccg gcggtcagca gcgagtagaa ctcgagctcg
     1681 cggtacagcg cgtcgagctg cttggggtcc ggcggggtca ggcggaggtc gtcgagggtg
     1741 acgtcgagct cggcgttgac gtcgacggtg gcgagctggc ggtagagctt gacggcctcg
     1801 gtgtgctcga cgagcgcggc cttttgtttg cccttgaggt cgtcgagatg ggcgaggatg
     1861 ccgtcgagcg agccatactt ctcgagcagg ccggcggcgc ccttttggcc gatgccgggc
     1921 acgccgggga tgttgtcgac gtcgtcgccc atcagcgcga gcaggtcggg gatcaggccg
     1981 ggggcgacgc cccacttctt gcgcacgagc tcggcgtcgt aggtgacgtc gcgcatcgtg
     2041 tcctgcatgc gcacgtggtc gccgaccatc tgcgcgaggt ccttgtcgcc ggcgacgatg
     2101 acgacctcga ggccagcctc gacgccgcgg cgcgcgaggg tggcgatgat gtcgtcggcc
     2161 tcgtagccgg ggcggcggat catcgggaaa tggttggcgg cgacgagtcg gtcgatcgtg
     2221 ctcagctgct gcgcgagctc gccgggcatc gacgggcgct gggccttgta ggccgggaac
     2281 ttgagctcgc ggtgagtcgc agccggggag tcgaacacga cggcgccgcg gtccggcatg
     2341 cgcccggaga acagcttgcg gaacatcgtg gcgaagccga agatcgcgtt cgtcggcaag
     2401 ccggtctttg tctgaagatt ggccgggatc gcaaagaagg cccgatagac gagccacgaa
     2461 ccatcgacca gcacgagccg ctctgccttc gccatgccgc ggatgatacg cgggaggcga
     2521 cttcgcgcgc gagcccgaag acgccgaaat taccgcgaaa ttcgccgcgc tttgcgcccg
     2581 gcccgcggtc agctggcggt cgcctgcgcc tcgatgccgg cctgctccgc gggcaacccg
     2641 aggtgccggc gaaactgctg ctcgagcgag tcgaggtact tccactgact catcgccgcc
     2701 gcgcgcatgc gatcgacgac cggctcgacg tcctcgacgt tgggcatggc ctcgccgccg
     2761 cggcgacgct ggatcgccga gaccgcgtgc tcgacaccga cgcgggcgcc gaggaacgcc
     2821 ccgagcaacg cgcgcttgaa cggggccggg tcgacctcct tgtaggtccg cagcatcgcc
     2881 ttgcggacct tttgcaggta gcgcgtgtcc tcgagcgagg ggccgggctt gccgccctta
     2941 ttgtcctgct cgagcaggcg agcgagcagc cagccgtcgt ggaacacgcc cgcggcgacg
     3001 tcggcgcagt cgcggatcgc cagcaggtac acgaccttgc gcaacagcct gcgcacgggg
     3061 aacagcgcga ccgaggtcgc cgcgccgcgc agccagaccg acggggcctc cgtcagcacg
     3121 cgctcgccgg aggtcgagag gaacagcccg tgcgcgtcgt acagcgcgct gtgcatgcgc
     3181 cgggtgaccc tgcggatcag gaagtcgtcg acgtagggca gcgggatcag cgggcacagc
     3241 cctgcgagcg cgctgtaggc cgcgacgatc cgcaacgatc ggggggaggt gtcgtgtgcg
     3301 tgtgtcatac gtcgccgtgg aggctaggcg aggtggcacg atcggccagt ggggtggggg
     3361 cggcggccgg acggatcgcc gtccccgggc cctggaccgg ggccagcagg cgcgtggtca
     3421 gacgcagggg cccggttgcg cggcgcgcga acaggccggg aacggccgct gcgggtgcgt
     3481 gcaacgcagt ttgctggcaa atggcgaagc caacataacc cccgaggata ccggcatatc
     3541 tccacacttg cggttgcgcc gggcccgcgg acgtggcctc ttcgtgcgcg tgaccgcgac
     3601 gaattacggt gtgctcgggc agctcgaggt tccgctcggg ctcggtctgc ttcgcctctc
     3661 gacggagggc cggcccgcgg agtcggacgc gatcgacgtg atccacttcg cgctcgatca
     3721 ggggatccgc gtgctcgaca cggcggactc gtacgcgctc gacgaccgcg acatgcatta
     3781 cggcgagcgt ctggtccgca aggcgatcgc ggcgtgggag ggcccgcgcg agcaggtccg
     3841 cgtgatcacc aaggccggcc tcgcgcggcc gaaggggcgc tgggtcccga acgcgcggcc
     3901 ggatcacctg cgcaagatgg tcgagggcag cctgacggcg ctgggcgtcg agcggatctt
     3961 cctgttgctg ctgcacgcga acgacccgaa gaccccgtac gaggacagcc tcggcgcgct
     4021 cgcgcagctg caaaaggagg ggaagatcga gcacctcggc ctgtcgaaca cctcgatcgc
     4081 ggaggtccgc caggccgagc ggcacttcgc ggtgtgtgcg atccagaacg agctcagcgt
     4141 catcgatcgc aacagcgggg tcgaggggct ggtcgcgctg gcgcggcgga tgaacatccc
     4201 gttcctcgcg caccggccgc tcggcgggca cgcgaagatc ggcaacctgc tcaagaaccg
     4261 ggcggtcaag ccgatcgcgg cgcgccacaa gatcacgccg cacgaggcgg ccctcgccgc
     4321 gctgctcgac ctcggcgcgc cggtgatccc gctgttcggc gcgacgaagc gggcgagcgt
     4381 cgagtcgagc ctgcgcgcgc tgaaggtgcc cttcgacgag cgcgaccggg cggagctcgg
     4441 ca
//
LOCUS       DASWNJ010000463         2204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_220327, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000463 DASWNJ010000000
VERSION     DASWNJ010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220327"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VGB85_07910"
     CDS             <1..306
                     /locus_tag="VGB85_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133988.1"
                     /translation="PNGCDCFGCCNIANKFYYLNSGPNCSINNLAACNTCTFFEQCAN
                     ACDTKNCELCFGQTIDDLPVECNDTPSCMNGVSCVDETDCAGGEFCQTGCCAKIVPQ"
     gene            complement(363..>2204)
                     /locus_tag="VGB85_07915"
     CDS             complement(363..>2204)
                     /locus_tag="VGB85_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133989.1"
                     /translation="VHTLMAEARDAAVRAGTTGKPDDAELALNLLGQILGRDPHYRDA
                     IGLTRALATQLTDPTPAVNLLSTVFGRISDPGLRYELGVFLGEQSGADEDAIAYFTVA
                     AQARPDGKQALRGLVRCYQQLGREAESANAMEQLLALYDPGEPSAVDLRLILAGILAQ
                     SQETMGRALDHARIVLAARGDDPRAIQLMANLLERNKLPAEAADILDRLAARERDPDR
                     LHDLYMQRAKLLADVPGREAQALAAVEKAAELGPGNRTTILLLIRLLERSGQVDRVAA
                     YLDPIRAAMLASIGRGTVAPTDIRMLAEVAARPNPALAQMARLLCYAIEPNSAPPPEG
                     HMRAATVNGIQQVLATPTLRQMLLSPGESADINDLLACVESAMDRMYVEFTGLGPDEI
                     VPVPPAVDALAFAAALQNWARLVGLTDLGLRATSINNTVALLEESDDPTLRLGVNLWL
                     RGDNVAWRGLAALALARRALGGGKVRALVPVELDLMLAASFEVTKVFNAITADPDTRR
                     LRDLTNNLGKNLPRRQRRPLLRICQALSSTHFEPAATARATLATDLRMALLLSGDVSG
                     CLAAACLLDGFANGSLKQRISLSRTAQELAVFMLSDAYLSLRRQMSV"
BASE COUNT          303 a          706 c          876 g          319 t
ORIGIN      
        1 cccaacggct gcgactgctt cggctgctgc aacatcgcca acaagttcta ctacctcaac
       61 tcgggcccga actgctcgat caacaacctc gcggcctgca acacctgcac gttcttcgag
      121 cagtgcgcca acgcctgcga caccaagaac tgcgagctgt gcttcggcca gacgatcgac
      181 gacctgccgg tcgagtgcaa cgacacgccg agctgcatga acggggtgtc ctgcgtcgac
      241 gagaccgact gcgccggcgg cgagttctgc cagaccggct gctgcgcgaa gatcgtcccg
      301 cagtgatggt cccgcggtga tccgacgagg cgaccgttcg cggtcgcctc gtttttggcg
      361 ggtcagaccg acatctggcg gcgcagtgac aggtaggcgt cgctgagcat gaacaccgcg
      421 agctcctggg cggtgcggct gaggctgatg cgctgcttga ggctgccgtt ggcgaagccg
      481 tcgagcaggc aggcggcggc gaggcagccg ctgacgtcgc ccgagagcag cagggccatg
      541 cgcaggtcgg tcgccagggt cgcgcgggcg gtcgcggcgg gctcgaagtg ggtgctcgag
      601 agcgcctggc agatgcgcag cagcgggcga cgctggcggc gtgggaggtt cttgccgagg
      661 ttgttggtca ggtcgcgcag gcgccgggtg tcggggtcgg ccgtgatcgc gttgaagacc
      721 ttggtgacct cgaagctggc ggcgagcatc aggtcgagct cgaccggcac gagcgcgcgg
      781 accttgccgc cgccgagggc gcgacgggcc agcgcgagcg cggcgaggcc acgccacgcg
      841 acgttgtcac cgcgcagcca caggttcacg ccgagacgca gggtcgggtc gtcgctctcc
      901 tcgagcagcg cgacggtgtt gttgatcgag gtggcgcgca ggccgaggtc ggtgaggccg
      961 acgaggcggg cccagttctg cagcgcggcc gcgaaggcga gcgcgtcgac ggccggcggc
     1021 accggcacga tctcgtcggg accgaggccg gtaaactcga cgtacatgcg gtccatcgcc
     1081 gactcgacgc aggcgagcag gtcgttgatg tccgccgact cgccgggcga cagcagcatc
     1141 tggcgcaggg tcggggtcgc cagcacctgc tggatgccgt tgacggtcgc ggcccgcatg
     1201 tggccctcgg gcggcggcgc cgagttgggc tcgatcgcgt agcacagcag gcgggccatc
     1261 tgcgccagcg cggggttcgg gcgcgcggcg acctcggcga gcatgcggat gtcggtcggc
     1321 gcgacggtgc cgcggccgat gctggcgagc atcgcggcgc ggatcgggtc gaggtaggcg
     1381 gcgacgcggt cgacttgacc gctgcgctcg agcaggcgga tcagcaggag gatcgtcgtg
     1441 cggttgccgg ggccgagctc cgcggccttc tcgacggcgg cgagcgcctg ggcctcgcgt
     1501 ccggggacgt cggccagcag cttggcgcgc tgcatgtaca ggtcgtgcag gcggtccggg
     1561 tcgcgctcgc gggcggcgag gcgatcgagg atgtcggcgg cctctgccgg gagcttgttg
     1621 cgctcgagca ggttggccat cagctggatc gcgcgcgggt cgtcgccgcg ggcggcgagc
     1681 acgatgcgcg cgtgatcgag ggcgcggccc atcgtctcct gcgactgcgc gaggatgccg
     1741 gcgaggatca ggcgcaggtc gaccgcggac ggctcgccgg ggtcgtagag ggcgagcagc
     1801 tgctccatcg cgttggcgga ctccgcctcg cggccgagct gttgatagca gcgcaccagg
     1861 ccgcgcagcg cctgcttgcc gtcggggcgc gcctgggcag cgacggtgaa gtaggcgatc
     1921 gcgtcctcgt cggcgccgga ctgctcgccg aggaacacac cgagctcgta gcgcaggccg
     1981 gggtcgctga tccggccgaa cacggtgctc agcaggttga cggccggcgt cgggtccgtc
     2041 agctgggtgg cgagcgcgcg ggtcaggccg atcgcgtcgc ggtagtgagg gtcgcggccg
     2101 aggatctggc cgagcaggtt caaggcgagc tcggcgtcgt cgggcttgcc ggtggtgccg
     2161 gcgcgcacgg ccgcgtcgcg ggcctcggcc atcagcgtgt gcac
//
LOCUS       DASWNJ010000464         2137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_432659, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000464 DASWNJ010000000
VERSION     DASWNJ010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432659"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..730
                     /locus_tag="VGB85_07920"
     CDS             131..730
                     /locus_tag="VGB85_07920"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY0133990.1"
                     /translation="MSRPIFALVLLVLFYAVAFGLRTWQHLRATGSTGFRGLSGRPGS
                     VEWLGGALFAAGVVLSLAAALGEAAGWLTTLWEPGVASVVLGTMITLGGIATTYLAQV
                     SMGDAWRIGVREEERTRFVTEGPFAVVRNPIFSCMSITAAGIVMLLPNPLSLASLVSL
                     LTAVELQVRFVEEPYLLRAHGPRYRDYMSRVGRFVPRLG"
     gene            741..1124
                     /locus_tag="VGB85_07925"
     CDS             741..1124
                     /locus_tag="VGB85_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133991.1"
                     /translation="MVHTLVRAVVLSTVTLFAFGCGQPKQDDAGQKSRPTAPAADAKR
                     TPDPKPAPGPGHAAAGVKPGSHEDWCGEHQVPESLCTRCDPSLIAAFKATSDWCAEHE
                     LPESQCRICNPDIKIVRPPAGEAKP"
     gene            1268..>2137
                     /locus_tag="VGB85_07930"
     CDS             1268..>2137
                     /locus_tag="VGB85_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133992.1"
                     /translation="MPPAICPKCNPVLAAVYQSKGDWCPEHGFPESLCPICNPGVKFP
                     DVGEPAAAPADWCGGHGLPESKCTKCNPSLVEQFKAAGDWCEEHGFPRSVCPVCNPQA
                     PPTSAAAMDWCVEHGLPESKCTKCNPALVEEYRAAGDYCAEHGFPESVCPVCNPVKPP
                     EGAEKAALEARTVRLRSPDLEAAAGIATVTVQRVEAVTSSVECTARLAFHGDRVAEIH
                     AVVPGIVRKVRVELGDRVKAGGDLFELERIHVGEIQAELQTTREGVRTAKANLARKRE
                     LLASELTSGRQVDL"
BASE COUNT          307 a          749 c          724 g          357 t
ORIGIN      
        1 gctagaacct gtgcgtcacc tcgcacgggc ccgcgcgctc gtgccgcttg accctgtagt
       61 cactacaggc tttagcctga cggcctcacg cggccacggc ccctctctga cgactaccat
      121 ggagttctcg atgtcccgcc cgatattcgc gctcgtcttg ctcgtcctgt tctacgcggt
      181 cgccttcggg cttcgcacct ggcaacacct gcgcgcgacc gggtcgaccg gatttcgcgg
      241 cctgtcgggt cggcccggct cggtggagtg gctcggcggc gcgctgttcg ccgcaggggt
      301 cgtcctcagc ctcgccgctg cgctcggtga agccgcgggc tggctcacca ccttgtggga
      361 gccgggcgtg gcgagcgtcg tcctcggcac gatgatcacg ctcggtggca tcgctacgac
      421 ctacctggcg caggtcagca tgggcgacgc ctggcgcatc ggcgtgcgcg aggaggaacg
      481 tacccggttc gtcaccgagg gcccgttcgc cgtcgtccgt aacccgatct tctcctgcat
      541 gtcgatcacg gcggccggga tcgtcatgtt gctcccgaat ccgttgtcgc tcgcgtcgct
      601 cgtgtccttg ctgaccgcgg tcgagttgca ggtccgcttc gtcgaggagc cgtacctgct
      661 gcgggcgcac ggtccgcgtt atcgcgatta catgtcgagg gtggggcgct tcgtgccccg
      721 actgggataa gggtttcgtc atggtccaca cactcgttcg agccgtcgtc ctgtccaccg
      781 tcaccctgtt cgcgttcggc tgcggccagc cgaagcaaga cgacgccggg cagaagtccc
      841 gacccaccgc gccggcagcc gatgcgaagc gcacgccgga tccgaagccg gcgccaggcc
      901 ccggacacgc ggccgccggt gtgaagcccg gctctcacga ggactggtgc ggcgaacacc
      961 aggtccccga gtcgctgtgc acgcgctgcg atccctcgct catcgcggcg ttcaaggcga
     1021 ccagcgactg gtgcgcggag cacgagcttc ccgagtcgca gtgccggatc tgcaacccgg
     1081 acatcaagat cgtccgtccg cctgccggcg aggcgaagcc gtgaggcggc tgttcggtct
     1141 tgtcctcgtg ctggtcctga tggtcgtcgc ggcatgcgac ggaaggagcg aggccccgcg
     1201 ggccaccgtg aacgaagcga ggccgccagc tgcctcgccg ccggctccgg gccggtgcga
     1261 acacggcatg cccccggcga tctgtcccaa gtgcaacccg gtgcttgcgg ccgtctatca
     1321 gtcgaagggt gactggtgcc cggagcacgg cttccccgag tcgctgtgtc cgatctgcaa
     1381 ccccggcgtg aaattccccg acgttggcga gcccgcggcc gcgccggccg actggtgcgg
     1441 cggtcatggc ctgcccgagt cgaagtgcac gaagtgcaac ccctcgctcg tcgagcaatt
     1501 caaggccgca ggcgactggt gcgaggagca cgggttcccc agatcggtct gcccggtctg
     1561 caacccgcag gcgccgccga cctcggcggc cgccatggac tggtgcgtgg agcacgggct
     1621 acccgagtcg aagtgcacga agtgtaatcc cgcgctcgtc gaggagtacc gcgccgcagg
     1681 cgactattgc gccgagcacg gctttccgga gtcggtctgt ccggtctgca accccgtgaa
     1741 gccgcccgag ggcgccgaaa aggccgcgct cgaggctcgc accgtccgcc tgagatcgcc
     1801 ggacctcgaa gccgcggcag gcatcgccac cgtcacggtc cagcgagtcg aggccgtgac
     1861 atcgtcggtc gaatgcacgg cacggctcgc gtttcacggc gatcgggtcg cggagatcca
     1921 cgcggtcgtg ccaggcatcg tccgcaaggt tcgcgtcgag ctgggtgacc gcgtcaaggc
     1981 tggtggcgac ctgttcgagc tcgagcgcat tcatgtcggc gagatccagg ccgagctgca
     2041 gaccacccgc gaaggcgttc gaaccgcgaa ggccaacctc gcgcgcaagc gcgagctgct
     2101 ggcgagcgag ctcacctccg gtcgtcaggt cgacctc
//
LOCUS       DASWNJ010000465         1948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_395400, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000465 DASWNJ010000000
VERSION     DASWNJ010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395400"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VGB85_07935"
     CDS             complement(<1..563)
                     /locus_tag="VGB85_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133993.1"
                     /translation="MTDESQPVPKDIRAELRAEASLLRPLAIAALCTYAYFIGPPAWN
                     QNSRLALTRALVEQRTTIIDPYHATTGDKSFRDGHFYSDKAPGASLLALPGYALVYGV
                     TLVAAGELPDVRVVPLDPGAELAGQQPEPEARAPGDRLAYNQPYRTALYVCRLLSTSL
                     LAVVGLGALYLISRRQLARGQAVLLTLL"
     gene            complement(1491..>1948)
                     /locus_tag="VGB85_07940"
     CDS             complement(1491..>1948)
                     /locus_tag="VGB85_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133994.1"
                     /translation="EHDMSQAGTKAYMAPERIVGTAPASVQSDLFGMAVVLWEMLKGQ
                     RCFKADDDLKTMDAIVKFDISHSTRRLTGLSSKLGDIVRKNLDRDPARRYSSAYQMLQ
                     RLAQTPEASAAERAQSELASLVREVSHLPASIATPPASAATTPARGPRS"
BASE COUNT          261 a          624 c          796 g          267 t
ORIGIN      
        1 agcagggtca gcagcaccgc ttgcccgcgc gccagctggc gccgcgagat caggtacagc
       61 gcgccgaggc cgacgaccgc gagcagcgag gtgctcagca ggcgacacac gtacaacgcg
      121 gtgcgatacg gctggttgta agcgaggcgg tccccggggg cgcgcgcctc gggctccggc
      181 tgctgacctg cgagctcggc cccggggtcg agcggcacga cccggacgtc cggtagctcg
      241 ccggcagcca ccagcgtcac gccatacacc agcgcgtagc ccggcagcgc cagcagcgag
      301 gcgccgggcg ccttgtcgct gtaaaaatgc ccgtcgcgaa agctcttgtc gccggtggtc
      361 gcgtgatatg ggtcgatgat ggtcgtgcgc tgctcgacca gcgcgcgcgt cagggccagc
      421 cgcgagttct ggttccatgc gggcgggccg atgaagtacg cgtaggtgca cagcgctgcg
      481 atcgccagcg ggcgcagcag cgaggcctcg gccctgagct cggcgcggat gtcctttggg
      541 acaggctgcg actcgtcagt catcgcgcct cccgggggcg gtgcgcggat ggccggtgct
      601 ggcggtcggg tctcgcgaga gcgggcgatg cggtgtgccc ggggctggcg tgcgcggcga
      661 tgcggtgcgc gggagcccgg ggatggcgtg gtcgggcgat gcggtgcgcg gatggccggg
      721 gctggcgtgc gcgggcgatt cggtgcgcgg ggatggcgtg gttggacgat gtggtgcgtg
      781 gatggccggg gctggcgtgc gcgggtgatg cggtgcgcgg gcggccacac cgggcgcgac
      841 cggaaagcgc ggggcgtcga tatctgggcc ggggagcggg ggccgacgga ctgcgggtcc
      901 ctgcggccca cggatctgcg ggccgccgat cgtccccagg tggggcgact gcgtgggctg
      961 atgggctgcc gcggtcatgc gacggttctg gcgcccgaca caagcggggc gcagacagtg
     1021 tccgcccggg gggccggggc cgactggcgg gggaagcagc gggaggaccc agggctcgcg
     1081 cgcagacgcc ccctcgtccg cccgatctcg ccacagaggc tcggtgagag agtgatgttg
     1141 tgcgcgcgag atggggcggc gagcacgtgc tctgggagga cccgcgtcgc agggacccgc
     1201 agcccgtcgg ccccccccgg cccaggtctc ctcgccccgc gtctaccgag cgcgcgacgg
     1261 ggcgttcggg caccgcgtct gccgagcgcg cgacgaggcg ttcgggcacc gcgtctaccg
     1321 agcgcgcgac ggggcgttcg ggcaccgcat cgcccgagcg cgcgacggga gcctccgggc
     1381 accacactac ccgagcaagc gaagggcacc gcattgctcg cgtacgcgaa cgagcacgag
     1441 cccgcgaggc atccggaccc tcgcgatccg aggagcgacg catcagcgca ctagctgcga
     1501 ggcccgcgag cgggggtggt cgcggcgctg gcgggaggag tggcgatcga ggcgggcagg
     1561 tggctgacct cgcggacgag gctggcgagc tcggactgcg cgcgttcggc cgcgctggcc
     1621 tcgggggtct gcgcgaggcg ctgcaacatc tgatacgccg agctgtagcg gcgggccggg
     1681 tcgcggtcga ggttcttgcg gacgatgtcg ccgagcttgg aggacagccc ggtcaggcgg
     1741 cgggtgctgt gcgagatgtc gaatttgacg atcgcgtcca tcgtcttgag gtcgtcgtcg
     1801 gccttgaagc agcgctggcc cttgagcatc tcccacagga cgacggccat gccgaacagg
     1861 tcggattgca ccgaggcagg agcagtaccg acgatgcgct cgggggccat gtacgccttc
     1921 gtgccggcct gtgacatgtc gtgttcct
//
LOCUS       DASWNJ010000466         6366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_360914, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000466 DASWNJ010000000
VERSION     DASWNJ010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6366
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360914"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VGB85_07945"
     CDS             complement(<1..314)
                     /locus_tag="VGB85_07945"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY0133995.1"
                     /translation="MITVHHLNNSRSQRVLWLLEELGLAYEVKRYERDAKTMLAPPEL
                     RAVHPLGKSPVITDEHRTVAESGAIIEYLVGRYGAGRLRPAEGTNERLRYDYWLHYAE
                     GSA"
     gene            319..483
                     /locus_tag="VGB85_07950"
     CDS             319..483
                     /locus_tag="VGB85_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0133996.1"
                     /translation="MREDATTPRGMHTPAPTIDDACQLASDRMRERSDLPSYANSCGQ
                     STGSVPAQGR"
     gene            complement(480..1883)
                     /locus_tag="VGB85_07955"
     CDS             complement(480..1883)
                     /locus_tag="VGB85_07955"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEY0133997.1"
                     /translation="MATGSTPRQREALTRQLAQGHTRVVAVGVDDYDADAGFPPRRTG
                     RNDALALANLFKDLPHLHAEPTAISTIVSRGEVVPSRGEVIRALRRLAQSAGPDDRLL
                     VFFSGHAHPIAGDLYLVPQDAFASDDPGCLISLSAIREVLRASTAKHKLVILDLEPTL
                     SRESLEAFFSKISGVAILSSAPGEARSPNPQSSPFTAHLLSALQGSAPAALDARLLTL
                     ASLHDYLCTVVPRPVLHAPRGTLVLADFSGPVLGAGAFDVDGKLFRRVGLSTGGRSVS
                     IKDILMSLSRTTNYTQEYLEAQANRALGPYLGEELGKKVSRLRARFRWSSSEVNVDGA
                     CITFPDGDYQPSYVATAKLQGVLIEELSLAPSWLEGPGMLADLLECLDVDPADVTFTL
                     AGRLVPATCVPRLEAHGWQITSELAHKIEAVNGRCVLRLTPETLALSGLPLAALFAAE
                     PDKEAVRTAAAALAILG"
     gene            complement(1933..2373)
                     /locus_tag="VGB85_07960"
     CDS             complement(1933..2373)
                     /locus_tag="VGB85_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0133998.1"
                     /translation="MPTKLSPHLIVAGAARAIEFYKKAFDARELSRYADEKRGGHIVC
                     AELTIGDQLFTVADEERAWKNDAPGSLGGSPVILNLEVPDVDAVGARLVAEGATVIYP
                     IADHFYGERSGRFQDPFGHLWLVTKKIKQMTPAEIQAGVDAYEG"
     gene            complement(2422..3246)
                     /locus_tag="VGB85_07965"
     CDS             complement(2422..3246)
                     /locus_tag="VGB85_07965"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0133999.1"
                     /translation="MPIVFRRPAAALAPFVAHLWHFEDPDGSLAHARERIMPDGTMQL
                     LVNLHEDELRSYHGEHGEPMELRRTGGAALCGPHTGHFAIDTAEQRAIMGVSFHPGGA
                     APFFAAPADAVCGLDVEVDRLWGRDGATLRERLLEQPTPAARLRTMEAVLLARVVRPL
                     ARDPAITLAIAALERDVPVAAVAERLGYSARRLISGFGAQVGLTPKRFARVRRFQRVL
                     RAITGGPPTPWVELALACGYFDQAHFIHDFRAFSGLHPTAYAPRDAAGHNHVILPD"
     gene            complement(3253..4701)
                     /locus_tag="VGB85_07970"
     CDS             complement(3253..4701)
                     /locus_tag="VGB85_07970"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEY0134000.1"
                     /translation="MGLLSAGAAHASGPNALADALDPQAIYGGTDVATCGWPTTVSME
                     GACSGTLVNPQVVIFAAHCGSNYNEVVLGESLNSPKRQLQVDFCKTYQGQQSGTDFAF
                     CKLKEPVLDVPIVPILMGCETEILQPGQEVTIVGFGDADTGPYGVKREVVTTINEIQN
                     NEAFIGGNGKDSCQGDSGGPVYVKLPDGSWRVFGITSYGGACGSGGYYSMMHLGMTWF
                     EEQSGIDLTPCHNADGSWQPGFGCQGFPTDPGPGGGAWPEGCGGGPVNAMSATCGGPF
                     DPTPDMTPPTVTVTQPLDGQELMGVDGVKVQVMIDAQDVGWGMKEVRLTIDGVEIDGG
                     VDNFPPYEFPLTFPTGAYCIGAIAVDLADNMGTATPVCIGVNGPPPMPPEPETTGDES
                     SSSGDLEGSTGDAEDTGTPPTTSAGATDDTGATGTPTTGVTSDSGNPTAGATGGEGEG
                     EGGCGCRSGHDPKDSLLALAGLGLLGLARRRR"
     gene            4902..6206
                     /locus_tag="VGB85_07975"
     CDS             4902..6206
                     /locus_tag="VGB85_07975"
                     /inference="COORDINATES: protein motif:HMM:NF015254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectin acetylesterase-family hydrolase"
                     /protein_id="HEY0134001.1"
                     /translation="MTTAPGSSRLWMALLLGSPGIACTASGGDSETATEASSSGTSAG
                     DSTTDATPTTTGGSTSGEPTSVTGEPDTTTGDDGPEPPAAYEKWLKVEIPGTVCGNGS
                     QYKFFINYKEGAKDLMVMLEPGGACWDYASCSGKSPLSAAHLDGVPDDLMTPNGQSAN
                     ISPLIRRDLGAPTADFNLVYVPYCTGDVHTGNNVITYEDPEGIGAPITFQHAGHANVL
                     AISEYLAKQFPTIDRLFITGCSAGGAGSLINYYFFRSKLAPARGYLMDDSGPIFPSTG
                     KSAPLHAEIRQSWNVDSIFAELPKEYPITDDFGVLNTHLADMFPEDRLSTIFFQRDFN
                     YSRYSYERFYPGANTKDDIMGYWASDTKLLVNLYQTRDNLGYYLPYWRALNDSHCVSI
                     ASYLDTDIEELGMNLGDYVNELFDDNAPMGRYMESFQPDEDD"
BASE COUNT         1018 a         2199 c         2221 g          928 t
ORIGIN      
        1 gccgagccct cggcgtaatg cagccagtaa tcgtagcgca gccgctcgtt ggtgccctcg
       61 gcgggccgca gccggcccgc accgtagcga ccgacgagat attcgataat ggcgcccgac
      121 tccgcgacgg tccggtgctc atcggtgatc accggcgatt tgccgagcgg atgcaccgcg
      181 cgcaactccg gcggcgcgag catcgtcttc gcgtcacgct cgtagcgctt cacctcgtac
      241 gcgaggccca gctcctcgag caaccagagc acgcgctgcg accgggaatt gttgaggtgg
      301 tgtacggtga tcatgcgcgt gcgcgaggat gccacgacac cgcgtggaat gcacaccccg
      361 gcgccgacga tcgacgacgc gtgtcagctc gcgtccgacc gcatgcgaga gcgctccgat
      421 ctgccctcgt acgcgaacag ctgcgggcag tcgaccggca gcgtgccggc gcaaggacgc
      481 tagccgagga tcgccagggc agccgcggcg gtgcgcacgg cctccttgtc gggctcggcg
      541 gcgaacaggg ccgcgagcgg caggccggac agcgcgaggg tctcgggcgt cagccgcagc
      601 acacatcggc cgttgacggc ctcgatcttg tgggcgagct cggaggtgat ctgccagccg
      661 tgggcctcga ggcgcggcac gcaggtcgca ggcacgagcc ggcccgcgag cgtgaaggtg
      721 acatcggcgg ggtcgacgtc gagacattcc aataggtcgg cgagcatgcc cggcccctcg
      781 agccacgacg gcgccagcga gagctcttca attaacacgc cctgcagctt cgcggtcgcc
      841 acataactcg gctggtagtc gccgtcggga aaagtgatgc acgcgccgtc gacgttgacc
      901 tcggatgatg accagcgaaa ccgtgcgcgc aggcggctga ccttcttgcc gagctcctcg
      961 ccgaggtacg ggccgagcgc gcggttggcc tgcgcctcga ggtactcctg cgtatagttc
     1021 gtcgtgcgcg acaggctcat caggatatct ttgatagata cgctgcggcc gccggtcgac
     1081 aggccgacgc ggcgaaacag cttgccgtcg acgtcgaagg cgccagcgcc gagcacaggc
     1141 ccgctgaagt cggcgagcac cagggtgcca cgcggcgcgt gcaggaccgg ccgcggcacc
     1201 accgtgcaca gataatcgtg caggctcgcc agcgtcagca gacgagcgtc gagcgccgcg
     1261 ggggccgagc cctgcagcgc ggacaacagg tgcgcggtga acgggctgct ctgaggattc
     1321 ggcgagcgcg cctcgccagg cgccgacgac aggatcgcca cgccgctgat ctttgaaaag
     1381 aatgcctcca atgattctcg cgacaatgtc ggctcgaggt cgaggatgac gagcttgtgc
     1441 ttcgcggtcg aggcgcgcag gacctcccgg atcgccgaga gcgagatcag gcagccgggg
     1501 tcatcgctgg cgaacgcgtc ctgcggcacg aggtacaggt caccggcgat cggatgcgcg
     1561 tggccactga aaaaaaccag cagccggtcg tcgggcccgg ccgactgcgc gagccttcgc
     1621 agcgcgcgga tgacctcgcc gcgcgacggg accacctccc cgcgcgacac gatcgtcgag
     1681 atcgccgtcg gctccgcgtg caggtgcggc aaatccttaa aaagattcgc gagggcgagg
     1741 gcgtcgttgc ggccggtgcg gcgcggtggg aagccggcgt ccgcgtcgta gtcgtcgacc
     1801 ccgaccgcga cgactcgcgt gtggccctgc gcgagctgcc gggtcagcgc ctcgcgttgc
     1861 cgcggtgtag atccggtcgc cacgagggct gcctggctag cacgccgcgc gacgcggtgg
     1921 caagggcgcg gcctagccct cgtaggcgtc gacgccggcc tggatctcgg ccggtgtcat
     1981 ctgcttgatc ttcttcgtca ccagccacag gtgcccgaac gggtcctgaa agcggccgga
     2041 gcgctcgcca taaaagtggt cggcgatcgg gtagatgacg gtcgcgccct ccgccacgag
     2101 gcgcgcgccg acggcgtcga cgtcgggcac ctcgaggttg aggatcaccg gcgacccacc
     2161 gagcgagccc ggcgcgtcgt tcttccacgc gcgctcctcg tcggcgaccg tgaacagctg
     2221 gtcgccgatc gtcagctcgg cgcacacgat gtgcccgcca cgcttctcgt cggcgtaacg
     2281 cgacagctcg cgcgcgtcga aggccttctt ataaaattcg atcgcacggg cggcgccggc
     2341 gacgatgagg tggggggaca gcttggtcgg catgcgcggc agtgtggatg ggagcgccgc
     2401 agagaggatt gtaaaaaatt gtcagtcggg gaggatgacg tggttgtggc cggctgcgtc
     2461 gcgcggggcg taggcggtcg ggtgcaggcc ggagaaggcc cggaagtcgt ggatgaagtg
     2521 ggcctgatca aaatagccgc aggcgagcgc cagctcgacc cacggcgtcg gtggaccgcc
     2581 ggtgatcgcc cgcagcacgc gctggaagcg gcgcacccgg gcgaagcgct tgggggtcag
     2641 gccgacctgc gcaccgaagc cggagatcag gcgccgggcg ctgtagccga ggcgctccgc
     2701 gaccgcggcg accggcacgt cgcgctcgag cgccgcgatc gccagcgtga tcgccgggtc
     2761 gcgcgcgagc gggcggacga cgcgggccag cagcacggcc tccatcgtgc gcaggcgggc
     2821 cgcgggcgtc ggctgctcga gcaggcgctc gcgcagcgtg gcgccgtcgc ggccccataa
     2881 ccggtcgacc tcgacgtcga gcccgcacac ggcgtcggcc ggtgcggcga agaacggggc
     2941 ggcgccgccc ggatgaaagc tgacgcccat gatggcgcgc tgctcggcgg tgtcgatcgc
     3001 aaagtggccg gtgtgcgggc cgcacagcgc ggcgccgccg gtgcgacgca gctccatcgg
     3061 ctcgccgtgc tcaccatgat aactccgcag ctcgtcctcg tggaggttga cgagcagctg
     3121 catggtgccg tcgggcatga tgcgctcgcg cgcgtgggcc aggctgccgt ccgggtcctc
     3181 gaagtgccac aggtgggcga cgaacggggc cagcgccgcc gccggccggc ggaacacgat
     3241 cggcatggcg gctcagcggc ggcggcgggc caggccgagc aggccgaggc ccgcgagcgc
     3301 gagcaggctg tccttcgggt catgtcccga acgacagccg cagccgccct cgccctcgcc
     3361 ctcgccgccg gtcgcgccgg cggtcgggtt gccgctgtcg ctggtgacgc cggtcgtcgg
     3421 cgtgccggtc gcgccggtgt catcggtcgc gccggcgctg gtggtcgggg gtgtgccggt
     3481 atcctcggcg tcgccggtcg agccctcgag gtcgccgctg ctgctgctct cgtcgccggt
     3541 ggtctcgggc tcggggggca tcggtggcgg gccgttgacg ccgatgcaca cgggcgtcgc
     3601 ggtgcccatg ttgtcggcga ggtcgaccgc gatcgcgccg atgcagtagg cgccggtcgg
     3661 gaaggtcagc gggaattcgt agggcgggaa gttgtcgacg ccgccgtcga tctcgacgcc
     3721 gtcgatcgtc aggcggacct ccttcatacc ccagccgacg tcctgcgcgt cgatcatcac
     3781 ctgcaccttg acgccatcga cgcccatgag ctcctgacca tcgagcggct gcgtcaccgt
     3841 gacggtcggc ggggtcatgt cgggcgtcgg gtcgaagggc ccgccacagg tcgcgctcat
     3901 cgcgttgacc ggaccgccgc cgcagccctc gggccaggcg ccgccgccgg gccccgggtc
     3961 ggtcgggaag ccctggcagc cgaagccggg ctgccagctg ccgtcggcgt tgtggcaggg
     4021 cgtgaggtcg atgcccgact gctcctcgaa ccatgtcatg ccgaggtgca tcatcgagta
     4081 gtagccgccg ctcccgcacg ctccgccgta ggaggtgatg ccgaacacgc gccagctgcc
     4141 gtccgggagc ttgacgtaca cgggcccgcc ggagtcgccc tgacagctgt ccttgccgtt
     4201 gccgccgatg aacgcctcgt tgttctggat ctcgttgatc gtggtgacga cctcgcgctt
     4261 gaccccgtac gggccggtgt cggcgtcgcc gaagccgacg atcgtgacct cctggccggg
     4321 ctggaggatc tcggtctcgc agcccatcag gatcggcacg atcgggacgt cgaggaccgg
     4381 ctccttgagc ttgcagaacg cgaagtcggt gcccgactgt tgaccctgat aggtcttgca
     4441 gaagtcgacc tgcagctggc gcttgggcga gttgagcgac tcgcccagca cgacctcgtt
     4501 gtagttgctg ccgcagtgcg ccgcgaagat caccacctgc gggttcacca gcgtccccga
     4561 gcacgccccc tccatcgaga ccgtcgtcgg ccagccgcac gtcgccacgt ccgtgccgcc
     4621 gtagatcgcc tggggatcga gcgcgtcagc gagggcgttc gggccgctcg cgtgcgctgc
     4681 accggccgac agcagcccca aaagacaagt ggtgaggcaa ctggtgaggg tccgtacgag
     4741 acatgccatc ctggcaccat cacccgcggg tcccgggaat gcaagacggc cccggcgaca
     4801 cgtcgactca catccgcggc caggccacga acaccacgcg ccgcacacgg gcccttacac
     4861 atctcgtgag ttttgctaag gtgcgcacgg agattgacga tatgacgacc gcgcccggca
     4921 gctcccgctt atggatggcc ctcttgctcg gctcgccggg catcgcatgc actgcatcgg
     4981 ggggcgactc cgagaccgcg acggaggcga gcagcagcgg caccagtgcg ggcgacagca
     5041 ccacggatgc cactccgacg acgaccggcg ggtccacttc gggcgagccg acctcggtca
     5101 cgggcgagcc ggacacgacg acgggcgacg acggccccga gccgccggcg gcctacgaga
     5161 agtggctcaa ggtcgagatc cctgggaccg tgtgcggcaa cggcagccag tacaagttct
     5221 tcatcaatta taaggagggc gccaaggacc tgatggtcat gctcgagcct ggcggcgcgt
     5281 gctgggacta cgcgagctgc tctggcaagt cgccgctgag cgcggcgcac ctcgacggcg
     5341 tgcccgacga cctgatgacg cccaatggcc agagcgccaa catctcgccg ctgatccgcc
     5401 gcgacctcgg ggcgccgacg gccgacttca acctcgtcta cgtgccctac tgcaccggcg
     5461 acgtgcacac cggcaacaac gtgatcacct acgaggaccc cgagggcatc ggcgcgccga
     5521 tcaccttcca gcacgccggc cacgccaacg tcctggcgat cagcgagtac ctcgccaagc
     5581 agttcccgac gatcgaccgc ctgttcatca ccggctgcag cgcgggcggg gccgggtcgc
     5641 tgatcaacta ctacttcttt cgcagcaagc tcgcgcccgc ccgcggctac ctgatggacg
     5701 actccggccc gatcttcccg agcaccggca agtcggcgcc gctgcacgcc gagatccgcc
     5761 agtcgtggaa cgtcgactcg atcttcgccg agctgcccaa ggagtacccg atcaccgacg
     5821 acttcggcgt gctcaacacc cacctcgccg acatgttccc ggaggatcgc ctgagcacga
     5881 tcttcttcca gcgcgacttc aactactcgc gctactcgta cgagcgcttc tacccggggg
     5941 ccaacaccaa ggacgacatc atgggctact gggcgtccga caccaagctg ctcgtcaacc
     6001 tctaccagac ccgcgacaac ctcgggtact acctgcccta ctggcgcgcc ctcaacgaca
     6061 gccactgcgt ctcgatcgcc tcgtacctcg acaccgacat cgaggagctc ggcatgaacc
     6121 tcggggacta cgtcaacgag ctgttcgacg acaacgcgcc gatgggccgc tacatggaga
     6181 gcttccagcc cgacgaggac gactgatgcg cgaccgcgcg cgggcgacat cccgccccgc
     6241 gccggttgac gggcgcaggc gccctgtggt gctctccccc cgggagagca cgcgccatgg
     6301 acctgagcgg acgctgggag tttggcttcg accgcagctt gaacggacag ctcgtaccca
     6361 cgagcg
//
LOCUS       DASWNJ010000467         1534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_44105, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000467 DASWNJ010000000
VERSION     DASWNJ010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44105"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1336)
                     /locus_tag="VGB85_07980"
     CDS             complement(<1..1336)
                     /locus_tag="VGB85_07980"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEY0134002.1"
                     /translation="MTPPFRKILVANRGEIALRVFRTCAEHGIRTVAVYSDADRDALH
                     ALQADEAVHIGGSTAAESYLQADKILAAAKATGAEAIHPGYGFLSERAHFVEACKAAG
                     VVFIGPDVRAMEVMGDKIGARKAMAAAGVPVVPGVNDIERYEQAEAAVHEIGYPVMLK
                     ASAGGGGKGMRIIHEAGELRRAYEAACREAKAAFGDDRMLMERAVLQARHVEIQLMAD
                     RHGTCLYLNERDCSVQRRHQKVIEESPSPSPQMTPEVRAAMGEVAVRAARAVDYAGAG
                     TVEFLFEEHPEGPRFYFLEMNTRLQVEHPVTEATCGRDLVWDQIRVAGGQRLGYTQAE
                     VELRGHAIECRLYAEDPRQFLPSPGDIRRLRWPVGAHIRIDSAVAEGSVVSRFYDPMI
                     AKMTVWGPDRLSALRRMREALKETVVLGIETNLAFHLRVLDEPDFLRGDFSTR"
BASE COUNT          212 a          552 c          544 g          226 t
ORIGIN      
        1 agcgggtcga gaagtcaccg cgcaaaaagt ccggctcgtc gagcacgcgc aggtgaaacg
       61 cgaggttggt ctcgatgccg agcacgaccg tctccttcag cgcctcgcgc atgcgtcgca
      121 gcgccgacag ccggtcgggc ccccacaccg tcatcttggc gatcatcggg tcgtagaagc
      181 gcgacacgac gctgccctcg gcgacggcgc tgtcgatccg gatgtgcgcg ccgaccggcc
      241 atcgcaggcg gcggatgtca cccgggctcg gcaagaactg tcgcgggtcc tcggcgtaca
      301 ggcggcactc gatcgcgtgg ccacgcagct cgacctccgc ctgggtgtag ccgagccgct
      361 gtccgccagc gacgcggatc tggtcccaca cgaggtcgcg gccgcaggtc gcctcggtca
      421 ccgggtgctc gacctgcagg cgcgtgttca tctccaggaa gtaaaagcgc gggccctccg
      481 ggtgctcctc gaacaggaac tcgaccgtgc ctgcccccgc gtagtcgacc gcacgcgcgg
      541 cgcggacggc cacctcgccc atcgcggcgc gcacctcggg ggtcatctgc ggactcgggc
      601 tcgggctctc ctcgatgacc ttctggtggc gccgctgcac cgagcagtca cgctcgttga
      661 ggtacaggca ggtgccgtgg cggtccgcca tcagctggat ctcgacgtgg cgggcctgga
      721 gcaccgcgcg ctccatcagc atgcggtcgt cgccgaacgc cgccttggcc tcgcggcacg
      781 cggcctcgta ggcgcgccgc agctcgccgg cctcgtggat gatgcgcatg cccttgccgc
      841 cgccgccggc ggaggccttg agcatcaccg ggtacccgat ctcatgcacc gcggcctcgg
      901 cctgctcgta gcgctcgatg tcgttgacgc cggggaccac cggcacgccc gcggccgcca
      961 tcgccttgcg ggcgccgatc ttgtcgccca tcacctccat cgcgcgcacg tcgggcccga
     1021 tgaacaccac gccggccgcc ttgcaggcct cgacgaagtg ggcgcgctcg ctgaggaagc
     1081 cgtagccggg gtggatcgcc tcggctccgg tcgccttcgc ggcggcgagg atcttgtcgg
     1141 cctgcaaata actctcggcg gccgtcgacc cgccgatgtg caccgcctcg tcggcttgca
     1201 acgcgtgcag ggcgtcacgg tcggcatcgc tgtacacggc gaccgtgcgg atgccgtgct
     1261 cggcgcaggt tcgaaacacg cgcagggcga tctccccgcg attggcgacc aggatcttgc
     1321 gaaacggcgg tgtcatggcg cggcgatcgc cttagcacag gcggccgcct ccgcggtcgc
     1381 aaatcggcgc acgcgacgga tcaacggggc gcacgcgacg gatcaacggg gcgcgcgcga
     1441 cggatcaacg gtgcgtcggc accgggcggg gcgaggtcgg cgggtgggcc tgggccgcgg
     1501 ctgcggccgc gggaccgtcc gggtcgagga tgtt
//
LOCUS       DASWNJ010000468         4944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_539129, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000468 DASWNJ010000000
VERSION     DASWNJ010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539129"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1601
                     /locus_tag="VGB85_07985"
     CDS             <1..1601
                     /locus_tag="VGB85_07985"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HEY0134003.1"
                     /translation="LRVVYAPAAGVGNYGGEVDNWRWPRHTGDFSFLRAYVGKDGKPA
                     DFSADNVPYKPKHHLKVATDKAAAGSFAMVVGYPGRTQRLKTAAEAQQAAEWYYNRQI
                     ALYEEHIAVLEAISKDRPEVAIKGAGRLRNLHNYLTNFKGVRDGLNKGGVASDKARIE
                     GELQKWIDADAGRKAKYGDVLTKMAALSQEKAKTREHDMAHDEILRGSSLLSIADTLV
                     KIADERQKPDAERKLGYQERDAKDLEQSFQAMQSSFDPDMDRAVFKLYLQRAAKLPEA
                     ERPELLAAVVGPGKTSDADIDRALTALYKSTKLGDPAARVALLKSATPATLKASRDPF
                     VKLALKIKPTVDAQKAKAEAQAGAMAALRPRYIEALQAFSGGNLAPDANSTLRITFGT
                     IRGYSPQPGAPEYTPFTKLSEVVAKHTGEEPFNAPKGLLDAVAAKKFGPYIDPTLGDV
                     PVNFLADLDITGGNSGSSTLNARGELTGLVFDGNIEAMASDWVFMPKVTRSIHVDIRY
                     VLWVMDAVDNADHLLTEMGVTPSI"
     gene            1676..1882
                     /gene="yacG"
                     /locus_tag="VGB85_07990"
     CDS             1676..1882
                     /gene="yacG"
                     /locus_tag="VGB85_07990"
                     /inference="COORDINATES: protein motif:HMM:NF015821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEY0134004.1"
                     /translation="MHIRCPICKREIKDAPDDFPARPFCSARCKTIDLGNWLSEAYRI
                     TRPLGPDDIDSLDEDDLIGERKPN"
     gene            1927..2844
                     /locus_tag="VGB85_07995"
     CDS             1927..2844
                     /locus_tag="VGB85_07995"
                     /inference="COORDINATES: protein motif:HMM:NF043005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCE4755 family polysaccharide monooxygenase-like
                     protein"
                     /protein_id="HEY0134005.1"
                     /translation="MLIRRGSCLVLGITLGCLAATTAQAHFVLQSPAALSEQNVLGDP
                     QKAPPCGDDGSAVATGMVTSYKAGDVITITIDETIFHPGHYRVALAMNDISELPEPPP
                     VTPADTDCGSAPIANPPVFPVLADGIFLHDQPFDGPQSFDITIPANVSCDNCTLQVIQ
                     FMSKHGLNNPGGCYYHHCANIAVEGGAGSTGGDESGGDNTTSTSTTATTAMTSDDTGT
                     PQTTADESTGNITGATGTGGDPTTGATATGGEPTTSGTGSGGESSSGNTNEGEGGCGC
                     TSSGSHAPATLLGLLGLLGLATRRRSRAR"
     gene            complement(3009..4886)
                     /locus_tag="VGB85_08000"
     CDS             complement(3009..4886)
                     /locus_tag="VGB85_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HEY0134006.1"
                     /translation="MAGRDSPGGTRGRGVVKRPAVFETISKGFRAARERMTGKGELTE
                     DVVNAALKDVRLSLLEADVEFGVVKRFLQNVKEKALGEKVQLVAKKKDETIRVRAEDH
                     FVKICHDELIALMGPVDTSLSRARKGVTGIMMVGLQGSGKTTTTGKLARKLQKAGYKV
                     MLVAADVYRPAAVHQLKVLGEKLGVPVFSIDGANPVEICADATDTAAAESYDFVIYDT
                     AGRLTIDQVLMQELEDIKTRVQPSNIFLVIDAMIGQDAVTTAKTFDERLDVTGVILTK
                     LDGDARGGAALSVKAVTGKPIKFLGIGEQLDKLDEFRPEGLASRILGMGDLVGLMNDF
                     QEVVDEETAAKDAEKMLSGNFSMDDFLTQIKTIQSMGSLKDIMEKMPLASMFGIDIPP
                     EAMQQATDDKELAKVEAMIRSMTKEERQNPEVFFDKEPKQSAASKLSRRKRREPTAGL
                     PDPNNMKAEDFVQSRITRVAKGCARSEEEVRGLIARFISMRDMFGMMGNMFGGLIPGM
                     PGGNQKKGGLGGMLDKIPGMGSLKQMNALRKLAQNPEALSQMFGGGGLPGMPGMGGLP
                     GMGGMPGLPGMGGMPGMPNLGGHGGMGGHSSAPAKNIDYKAIAAKRKAEKLARKKNRR
                     K"
BASE COUNT          787 a         1827 c         1575 g          755 t
ORIGIN      
        1 acctgcgcgt cgtctatgcg cccgcggccg gcgtcggcaa ctacggcggc gaggtcgaca
       61 actggcgctg gccccggcac accggcgact tctcgttcct gcgcgcctac gtcggcaagg
      121 acggcaagcc ggcggacttc agcgccgaca acgtccccta caagcccaag catcacctca
      181 aggtcgccac cgacaaggcc gccgccggca gcttcgcgat ggtcgtcggt taccccggcc
      241 gcacccagcg gctcaagacc gccgccgagg cccagcaggc cgccgagtgg tactacaacc
      301 gccagatcgc cctgtatgaa gagcacatcg ccgtgctcga ggcgatcagc aaggaccgcc
      361 ccgaggtcgc catcaagggc gccgggcgcc tgcggaacct gcacaactac ctcaccaact
      421 tcaagggcgt gcgcgacggc ctcaacaagg gcggcgtcgc cagcgacaag gcccgcatcg
      481 agggcgagct gcagaagtgg atcgacgccg acgccggtcg caaggccaag tacggcgacg
      541 tgctgaccaa gatggccgcg ctgagccagg agaaggccaa gacccgcgag cacgacatgg
      601 cccacgacga gatcctgcgc ggctcgtcgc tgctgtcgat cgccgacacc ctggtcaaga
      661 tcgccgacga gcggcaaaag ccggacgccg agcgcaagct cggctaccag gagcgcgacg
      721 ccaaggacct cgagcagagc ttccaggcga tgcagagctc cttcgacccc gacatggacc
      781 gcgcggtgtt caagctgtac ctgcagcgcg ccgccaagct gcccgaggcc gagcgccccg
      841 agctgctcgc cgcggtcgtc ggccccggca agaccagcga cgccgacatc gacagggccc
      901 tgaccgccct ctacaagtcg accaagctcg gcgaccccgc cgcgcgcgtc gccctgctca
      961 agagcgcgac cccggcgacg ctcaaggcca gccgcgaccc cttcgtcaag ctcgccctca
     1021 agatcaagcc gacggtcgac gcgcagaagg ccaaggcgga ggcccaggcc ggcgcgatgg
     1081 cggccctgcg tccgcgctac atcgaggcgc tgcaggcctt ctccggcggc aacctcgccc
     1141 cggacgccaa cagcaccctg cggatcacct tcggcacgat ccgcggctac agcccgcagc
     1201 ccggcgcgcc cgagtacacc ccgttcacca agctctccga ggtcgtcgcc aagcacaccg
     1261 gcgaggagcc gttcaacgcg ccgaagggcc tgctcgacgc cgtcgccgcc aagaagttcg
     1321 ggccctacat cgacccgacg ctcggcgatg tcccggtgaa cttcctcgcc gacctcgaca
     1381 tcaccggcgg caactccgga tcgtcgaccc tcaacgcgcg cggcgagctg accggcctgg
     1441 tgttcgacgg caacatcgag gcgatggcga gcgactgggt gttcatgccc aaggtcacgc
     1501 ggtcgatcca cgtcgatatt cgctacgtcc tgtgggtgat ggacgcggtc gacaacgccg
     1561 accacctgct gaccgagatg ggcgtcacgc cctcgatctg acgctctgcg cggcgacgac
     1621 ggcgccccgg ctcgcacgac ttgcgcgcgc cggggcgagc cgttatgtag cggacgtgca
     1681 catccgctgc ccgatctgca agcgcgagat caaggacgcc ccggacgact tccccgcgcg
     1741 cccgttctgc tccgcccgct gcaagaccat cgacctcggc aactggctca gcgaggccta
     1801 ccgcatcacg cgcccgctcg gcccggacga catcgacagc ctcgacgagg atgacctgat
     1861 cggcgagcgc aagcccaact gatacggcgg ctcgccgcgt cggcgaggct cgtgtacctt
     1921 cgagcgatgc tcattcgccg cggttcgtgt cttgtcctcg ggatcactct cggctgcctg
     1981 gcagccacca cggcgcaggc ccacttcgtg ctgcagtcgc ccgcggcgct cagcgagcag
     2041 aacgtgctcg gtgacccaca aaaggcgccc ccgtgcggcg acgacggcag cgcggtcgcc
     2101 acgggcatgg tgacctccta taaagccggc gacgtcatca cgatcacgat cgacgagacg
     2161 atcttccacc ccggccacta ccgcgtcgcg ctggcgatga acgacatcag cgagctgccc
     2221 gagccgccgc cggtcacccc ggccgacacc gactgcggca gcgcgccgat cgcgaacccg
     2281 cccgtgttcc cggtgctcgc cgacggcatc ttcctgcacg accagccctt cgacggaccg
     2341 cagtcgttcg acatcaccat tccggccaac gtcagctgcg acaactgcac gctgcaggtc
     2401 atccagttca tgtccaagca cgggctcaac aaccccggcg gctgttatta tcatcattgt
     2461 gccaacatcg ccgtcgaggg cggcgctggt agcacgggcg gcgacgagtc cggcggcgac
     2521 aacaccacga gcacgtccac gaccgcgacg accgcgatga cctcggacga caccgggacc
     2581 ccgcagacga ccgccgacga gagcaccggc aacatcaccg gcgcgaccgg caccggcgga
     2641 gacccgacga ccggcgcgac cgcgaccggc ggcgagccga cgacatcggg cacgggctcc
     2701 ggcggcgaga gctcgtcggg caacacgaac gagggcgagg gcggctgcgg ctgcacatcg
     2761 tccggcagcc acgcccccgc gacgctgctg ggcctgctgg gcctgctcgg tttggccacg
     2821 cgccgcaggt cccgagcccg ctgagcgctg cgcggcgccg gcggtcatcg tcggcgcccg
     2881 gatgctcggc gtgaccagca cgcgcacgtc ttcttccagt ggagcgcgtg ctcgacgtgc
     2941 ggccacacgc ggggcagggc gagcgaccgg acctcgccgg tcacccgctc gatcatggct
     3001 ccgccgagct acttcctgcg gttcttcttg cgcgcgagct tctcggcctt gcgcttggcg
     3061 gcgatcgcct tgtagtcgat gttcttggcc ggcgcgctcg agtgcccgcc catgccgccg
     3121 tggccgccga ggttcggcat gccaggcatg ccgcccatgc ccggaagacc cggcatgccg
     3181 cccatgcccg gcagtccgcc catgccgggc atgccgggca aaccgccgcc gccgaacatc
     3241 tggctcaggg cctccgggtt ctgggccagc ttgcgcagcg cgttcatctg cttgaggctg
     3301 cccatgccgg ggatcttgtc gagcatgccg ccgaggccgc ccttcttctg gttgccgccg
     3361 ggcatgccgg ggatcaggcc gccgaacatg ttgcccatca tgccgaacat gtcgcgcatg
     3421 ctgatgaagc gggcgatcag gccgcggacc tcctcctcgg agcgcgcgca gcccttggcc
     3481 acgcgggtga tgcggctctg cacgaagtcc tcggccttca tgttgttcgg gtcgggcagg
     3541 cccgcggtcg gctcacgccg cttgcggcgg ctgagcttcg acgcggccga ctgcttcggc
     3601 tccttgtcga agaacacctc ggggttttgc cgctcctcct tggtcatcga gcggatcatc
     3661 gcctcgacct tggcgagctc cttgtcgtcg gtggcttgct gcatcgcctc cggcgggatg
     3721 tcgatgccga acatgctcgc caggggcatc ttctccatga tgtccttgag gctgcccatg
     3781 ctctggatcg tcttgatctg ggtcaggaag tcgtccatcg agaagttgcc cgagagcatc
     3841 ttctccgcgt ccttggccgc ggtctcctcg tcgacgacct cctggaagtc gttcatcagg
     3901 ccgacgaggt cgcccatgcc gaggatccgg ctggcgaggc cctcggggcg gaactcgtcg
     3961 agcttgtcga gctgctcgcc gatgccgagg aacttgatcg gcttgccggt gaccgccttg
     4021 accgagagcg ccgcgccgcc gcgggcgtcg ccgtcgagct tcgtcaggat cacgccggtg
     4081 acgtcgaggc gctcgtcgaa cgtcttcgcg gtcgtgaccg cgtcctggcc gatcatcgcg
     4141 tcgatcacca ggaagatgtt cgacggctgc accctcgtct tgatgtcctc gagctcctgc
     4201 atcaacacct ggtcgatcgt caggcgaccg gcggtgtcgt agatgacgaa gtcgtagctc
     4261 tcggccgccg cggtgtcggt cgcgtcggcg cagatctcga ccgggttggc gccgtcgatc
     4321 gagaacaccg gcacgccgag cttctcgccg agcaccttga gctgatgcac ggccgccggg
     4381 cggtacacgt ccgccgcgac cagcatcacc ttgtagccgg ccttttgcag cttgcgcgcg
     4441 agcttgccgg tcgtcgtcgt cttgcccgag ccctgcaggc cgaccatcat gatgccggtc
     4501 acgcccttgc gcgcgcgcga cagcgaggta tcgacgggac ccatgagggc gatcagctcg
     4561 tcgtgacaga tcttgacgaa gtggtcctcc gcgcggacgc ggatcgtctc gtccttcttc
     4621 ttggcgacga gctgcacctt ctcgccgagc gccttctcct tgacgttctg gaggaagcgc
     4681 ttgacgacgc cgaactcgac gtcggcctcg agcaggctca ggcgcacgtc cttgagcgcg
     4741 gcgttgacca catcctcggt cagctcgccc ttgccagtca tgcgctcgcg cgcagcacgg
     4801 aatcccttcg aaatggtctc gaacacggcg gggcgtttta ccacgccccg gccccgcgtg
     4861 ccccctggtg aatctcggcc cgccacgtta cgtttgctcc gcccccatgc gtccgtcgca
     4921 agcccccccg gagacctcgc ggcc
//
LOCUS       DASWNJ010000469         6671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_185338, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000469 DASWNJ010000000
VERSION     DASWNJ010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6671
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185338"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..1686
                     /gene="ftsW"
                     /locus_tag="VGB85_08005"
     CDS             295..1686
                     /gene="ftsW"
                     /locus_tag="VGB85_08005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEY0134007.1"
                     /translation="MSTAATSAGTEADAIAEAAEAGVTASHVVDDIGDARDGGSPAVG
                     DERRAMDPWLFFAAMALSCIGLVMVYSAGVYTARVRHDNWEFFLERQGLFFALGTAAM
                     LGVSRIDYRVFRKFAGLFMGVALLGLVAVLFVGVEVNGARRWIRFPGMNIQPSELAKV
                     ALAVFLSTMLARQGERVRRFKVGFLPPLVVASVTMLLVLLERDLGTTILLGALTMTLL
                     FVAGTRIAYVLAAAMLAAPLVWHQIVDVGYRKGRLLDYLSGEQSYQIQQSLIAVGSGG
                     PWGLGLGAGRQKLGFLPENHTDFILASIGEELGLAGISVVIALFCLLVWRGLVTARNA
                     PDRFGTYLAIALSALFGFQAMINMAVVLDVIPAKGITLPFLSYGGSSLLVSMGAVGIL
                     LAISRRPRPWRISDQRGSRAPATAAAPGSTRAQRIAQARGQAPTTTGMLRRLWLFRKP
                     EPAPARRANVRRG"
     gene            complement(1790..2362)
                     /locus_tag="VGB85_08010"
     CDS             complement(1790..2362)
                     /locus_tag="VGB85_08010"
                     /EC_number="2.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF040696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-S-isoprenylcysteine O-methyltransferase"
                     /protein_id="HEY0134008.1"
                     /translation="MPSFTALYFAGMLAEIVIRAPYDRVRKSEVMRERRFTTQEQVLL
                     LALMLGMFVLPLVYATTDWLAFADVALPAWAGWLGVVLLAGALFVFWRGHRDLGRNWS
                     PTLEVRAQHELVTGGIYGVIRHPMYASQWLWALAQPLLLHNWLAGAAGLVVFVPFYFL
                     RVRAEERMMLDLFGDAYRRYMREVGGVLPR"
     gene            complement(2400..3140)
                     /locus_tag="VGB85_08015"
     CDS             complement(2400..3140)
                     /locus_tag="VGB85_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134009.1"
                     /translation="MKRATRTLIGIVTAAVLFAGACSKSVEGENQRWDTFSTQVDRLA
                     ATYPGFRPAIEARKAAAKKQHDAAATLPDDQKIAQLSAANATLMEGFVNDLDFIDDEM
                     KKLRESRVEVAANTSEDTRLGAKVAAEDAQRALDRVEAALQTGATDEAAANALLQKLR
                     TDLGTAQSALDKVLAIDQSKQTKAADAKQSEASKKAADEAAAAAKVAPWKCSYCSSEN
                     PHDATSCNSCGAPKADAKADAKAGTPAK"
     gene            3251..6601
                     /locus_tag="VGB85_08020"
     CDS             3251..6601
                     /locus_tag="VGB85_08020"
                     /inference="COORDINATES: protein motif:HMM:NF020076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEY0134010.1"
                     /translation="MRNIPLVVRLALPGLMFSCTGSPGPAEGSQSSGECSLLWNDAGG
                     SSIGVAPFSLTTQDGTGLELVELVSRAHVEDPLAFTELHLSFRNPNDRVIEGRFEINL
                     PPNSAISRFAMKIGGRWQEAEVVELQAARAAYEDALHRKVDPALLEKAAGNRFSARVF
                     PIPADDVKEIIVAYSEELTASGAPYRVYLRGLPKLQSMDVEVVVPRPGAAPERTRVTR
                     TDFMPEHDLELSTPRRSSEVGLRFDRLVVARIAPDIKLPDEPLTGVTLLFDTSASRAL
                     DFKGQVARLMTLIKELRAANGTDFPLTVAAFDQSVEVVFTGAAEQFESDAVQALLKRG
                     PLGASDLTGALRWAATQKLPPRVIIVGDGVATAGDTGRDALRKAASDLAGAGAQRLDA
                     VVDGGLRDDSVLRQLTTAALPQAGIVVDGGLPAPLVASRLKRATRANVAVRVPGATWV
                     WPSTLASVQPGDQFLVFADLPADQAMRVELGAGEDVREPVLTSSTRPLLERAWIRASI
                     ERLSTMLGEEPEAKADLVKQIVEMSTRYRVLSDYTALLVLETEQDYERFGIPRTALAE
                     ILTVGPDGITVVDRNTLPRKPGEAPWVVLADRDFEYEDDEQDEPRTQLDLVRAPLDRL
                     LGIERAHDPEDDPIPEDQENTDEEAGGSSHRHRGEEGRMGKPVPHFKRGLYAMKGPGD
                     VGPPSEEASDGRFEEEAIEADTKRGPPQSREVASAAHVEAEPPGEPRMDDRPTPSPAK
                     KRVSRDPPARRRSPVTSEDSDGPITRPTPTFEKPRKADPWEGRFAAVMAEIGADRDAA
                     ALAQALAWRASSPGDEIALIALGEAAEASGDLTLAARAYGSLIDLFPGRADLRRMAGA
                     HLEALGEVGLALAIDTYRQAVAQRPDHPASHRLLAFALSKAGRHAEAFAAALAGAQRS
                     YPPDRFAGVQRILREDLALLAAAWRAAQPGDKAMLAALAQAGVKPDTRPSLRFVLNWE
                     TDANDVDFHVYDGKGGHAYYVAKLLPGAGELYDDVRTGYGPECFAIAGEPDAFPYQLQ
                     AHYYSRGPMGYGMGKLQAIEHDGEGKLVFKEHPFVIMKDRAFVDLGRVDAPLAKLPAD
                     QIRLPSGAGEGKAPVGTYTPPPPPMPAR"
BASE COUNT          940 a         2325 c         2432 g          974 t
ORIGIN      
        1 atcagcgcgg ggagtcgccc gggaaccttc ccccggagcc tcgccggaag cctctgcgtg
       61 tgcgtacgaa gcatggtgga gagcgtggcc cgaaggatcg ccaaggccag cggaattcgc
      121 aagccctgcg ccgatgacag cgtggcagaa agcggtgacg aagtggcacg cagcgacacg
      181 ccgaaaggga ccttcgcggc gcgcgcgcga caattgggca cgcgctcgcc cgaacgtccc
      241 ccgggtgcgc cgattttttg ccagcgcccg cgaagcgtgc gtaggtccgg gttcgtgagc
      301 accgcggcca ccagcgcagg gaccgaagcc gacgcgatcg ccgaggccgc cgaggcgggc
      361 gtcaccgcga gtcacgtcgt cgatgacatc ggcgacgccc gtgacggcgg ttcgccggcg
      421 gtcggcgacg agcggcgggc gatggacccg tggctgttct tcgcggcgat ggcgctgtcg
      481 tgcatcggcc tggtgatggt ctacagcgcg ggcgtgtaca cggcgcgtgt gcgccacgac
      541 aactgggagt tcttcctcga gcgccagggg ttgttcttcg cgctcggcac cgcggcgatg
      601 ctcggggtct cgcggatcga ctatcgcgtg tttcgcaagt tcgccgggct gttcatgggc
      661 gtcgcgctgc tcgggctggt cgcggtgttg ttcgtcggcg tcgaggtcaa cggcgcgcgc
      721 cggtggatcc ggttcccggg catgaacatc cagccgagcg agctcgccaa ggtggcgctc
      781 gcggtcttcc tgtcgacgat gctcgcgcgt cagggcgagc gcgtgcgtcg cttcaaggtc
      841 ggcttcctgc cgccgttggt ggtcgcgtcg gtgacgatgc tgctggtgtt gctcgagcgc
      901 gacctcggca ccacgatcct cctcggcgcg ctgacgatga cgctgctgtt cgtcgccggc
      961 acgcgcatcg cctacgtgct cgcggcggcg atgctcgcgg cgccgctggt gtggcatcag
     1021 atcgtcgacg tcggctatcg caagggccgc ctgctcgact acctctcggg cgagcagtcg
     1081 tatcagatcc agcagtcgct gatcgcggtc ggctcgggcg ggccgtgggg gctcgggctc
     1141 ggcgcgggcc gccagaagct cggatttttg cccgagaacc acaccgactt catcctcgcc
     1201 agcatcggcg aggagctcgg gctcgcgggc atctcggtcg tcatcgcgct gttctgcctg
     1261 ctggtgtggc gcggcctggt gaccgcccgc aacgcgccgg accgcttcgg cacatacctg
     1321 gcgatcgcac tctcggcgct gttcgggttc caggcgatga tcaacatggc ggtcgtgctc
     1381 gacgtgatcc cggccaaggg catcacgctg ccgtttttgt cctacggtgg atcgtcgctg
     1441 ctggtcagca tgggcgccgt cggcatcttg ctggcgatct cgcggcgacc ccggccatgg
     1501 cggatctccg accagcgcgg cagtcgagcg cccgcgaccg cggccgcgcc cggctcgacg
     1561 cgggcccagc ggatcgccca ggcgcgcggc caggcgccga ccacgaccgg tatgcttcgg
     1621 cggctgtggc tgttccgcaa gcccgagccc gcgccggcgc gccgcgccaa cgtgcgtcgc
     1681 ggatgagcgg tgagccgccg cgtcgcacgc cgatagttca tcgtgaacgt tgtagtcgcg
     1741 gccgggctgg cgtcgtcacg ttgatggtcg gcgtcgcccg ctgagcagtt caccgcggga
     1801 gcacgccgcc gacctctcgc atgtatcggc gataggcgtc tccgaacagg tcgagcatca
     1861 tgcgctcctc ggcgcgcacg cgcaggaagt agaacgggac gaacacgacg aggccggcgg
     1921 cgccggccag ccagttgtgc agcagcaacg gctgggcgag cgcccacaac cactgcgagg
     1981 cgtacatcgg gtggcggatg accccgtaga taccgccagt gaccagctcg tgctgcgcgc
     2041 ggacctcgag cgtcggcgac cagttgcgcc cgaggtcccg gtgcccgcgc cagaacacga
     2101 acagcgcgcc cgcgaggagc accacgccga gccagccggc ccacgcaggc aaggcgacat
     2161 cggcgaaggc gagccagtcg gtggtcgcgt ataccagcgg caacacgaac atgccgagca
     2221 tcagcgcgag cagcaggacc tgctcttgcg tagtgaaccg ccgctcgcgc atgacctccg
     2281 acttgcggac gcggtcgtac ggggcacgaa tgacgatctc cgcgagcatg ccggcgaagt
     2341 agagcgcggt gaagctgggc atcgaggttc ctccgatcgc gagcttggcg cggcgcgcct
     2401 cacttggccg gcgtgccggc cttcgcgtcg gccttcgcgt cggccttcgg tgcgccacac
     2461 gagttgcagg acgtcgcgtc gtgcgggttc tcggagctgc aatagctgca cttccacggg
     2521 gcgaccttgg cggcggcggc ggcctcgtcg gcggcctttt tgctcgcctc ggactgcttg
     2581 gcgtcggcgg ccttggtctg cttcgactgg tcgatcgcca gcaccttgtc gagggcggac
     2641 tgcgcggtgc cgaggtcggt ccggagcttc tgcaggagcg cgttcgcggc ggcctcgtcg
     2701 gtcgcgccgg tctggagggc ggcctcgacg cggtcgaggg cccgctgggc gtcctcggcg
     2761 gccaccttgg cgccgaggcg ggtgtcctcg ctcgtgttcg cggcgacctc gacccggctc
     2821 tcgcgcagct tcttcatctc gtcgtcgatg aagtcgaggt cgttgacgaa gccctccatc
     2881 agggtcgcgt tcgcggcgga cagctgcgcg atcttctggt cgtccgggag ggtggcggcg
     2941 gcgtcgtgtt gcttcttcgc ggcggccttg cgcgcctcga tcgcggggcg aaagccgggg
     3001 tacgtcgccg cgagccggtc gacctgcgtc gagaacgtgt cccagcgctg attctcgccc
     3061 tcgacgctct tgctgcaggc ccccgcgaac aacaccgccg ctgtcacgat cccgatcagt
     3121 gtcctcgttg cgcgcttcat cgccctcttt tacgcgatcg caacgcgacg cacgaggtcc
     3181 gacttcgcgc ggcgacgggc ctgccatgcg acgagcgatc tttcaggtac cttcgatcgt
     3241 ggagacatcc atgcgcaaca tcccgctcgt cgtccgcctc gcgctccccg gactcatgtt
     3301 ctcgtgcacc ggcagcccgg gtccggccga gggatcgcag tcgagcggcg aatgctcgtt
     3361 gctgtggaac gacgccggcg gatcgtcgat cggggtggcc ccgttctcgc tgacgaccca
     3421 ggacgggacc gggctcgagc tggtcgagct ggtcagccgc gcgcacgtcg aggacccgct
     3481 ggccttcacc gagctgcacc tgagctttcg taacccgaac gatcgcgtga tcgagggccg
     3541 cttcgagatc aatctccccc cgaactcggc gatctcgcgc ttcgccatga agatcggcgg
     3601 gcgctggcag gaggccgagg tcgtcgagct gcaggcggcc cgcgccgcgt acgaggacgc
     3661 gctgcaccgc aaggtcgacc cggccctgct cgagaaggcc gcaggcaacc gcttcagcgc
     3721 ccgcgtgttc ccgatcccgg ccgacgacgt caaggagatc atcgtcgcgt actccgagga
     3781 gctgacggcc agcggggcgc cctatcgcgt gtacctgcgc ggactgccga agttgcagtc
     3841 gatggacgtc gaggtcgtgg tcccgcgccc gggcgcggcc ccggagcgca cgcgcgtgac
     3901 gaggaccgac ttcatgccgg agcacgacct cgagctgagc acgccgcggc gcagcagcga
     3961 ggtcggcctg cgcttcgatc gcctggtcgt cgcgcggatc gccccggaca tcaagctgcc
     4021 cgatgagcca ctgacgggcg tcacgctgct gttcgacacc agcgcctcgc gggcgctcga
     4081 cttcaagggc caggtggcgc gcctgatgac gctgatcaag gagctgcgcg cggccaacgg
     4141 cacggacttc ccgctgacgg tcgccgcctt cgaccagagc gtcgaggtgg tgttcaccgg
     4201 cgcggccgaa cagttcgaat ccgacgcagt ccaggcgttg ctcaaacgcg ggccgctcgg
     4261 cgcctcggat ctgacgggcg cgctgcgctg ggccgcgacc cagaagctgc cgccgcgggt
     4321 gatcatcgtc ggcgacgggg tggcgaccgc gggtgacacc ggacgcgacg cgctgcgcaa
     4381 ggcggccagc gacctcgccg gcgcgggcgc gcagcgcctc gacgcggtcg tcgacggcgg
     4441 cctgcgcgac gattccgtgc tgcgccagct gacgaccgcc gcgctgccgc aggccggcat
     4501 cgtggtcgac gggggcctgc cggcgcccct ggtggcctcg cgcctgaagc gggcgacgcg
     4561 ggcgaacgtc gcggtgcgcg tgcccggggc gacgtgggtg tggccgtcga cgctcgcgtc
     4621 ggtgcagccg ggcgatcagt tcctggtgtt cgccgacctg cccgcggacc aggcgatgcg
     4681 cgtcgagctc ggcgccggcg aggacgtgcg cgagccggtg ctgacgagca gcacgcgacc
     4741 gctgctcgag cgcgcgtgga tccgcgcgag catcgagcgc ctgtcgacga tgctcggcga
     4801 ggaaccggag gcgaaggccg acctcgtgaa gcagatcgtc gagatgtcga cgcgctaccg
     4861 cgtgctctcg gactacacgg cgctgctcgt gctcgagacg gagcaggact acgagcgctt
     4921 cggcatccca cgcaccgcgc tcgccgagat cctcacggtc gggcccgacg gcatcaccgt
     4981 ggtcgaccgc aacaccctgc cgcgcaagcc cggtgaggcg ccgtgggtgg tgctcgcgga
     5041 ccgcgacttc gagtacgagg acgacgagca ggatgagccg cgcacccagc tcgacctggt
     5101 ccgcgcgccg ctcgaccgcc tgctcggcat cgaacgcgcg cacgacccgg aggacgaccc
     5161 gatacccgag gaccaggaga acaccgacga ggaggccggc ggctcgagcc accgccaccg
     5221 cggcgaggag ggccggatgg gcaagccggt cccgcacttc aagagggggc tgtatgcgat
     5281 gaagggcccc ggggacgtcg gcccgccgag cgaggaagcg agcgatggcc gcttcgagga
     5341 ggaagcgatc gaggcggaca ccaagcgcgg cccgccgcag tcccgcgagg tcgcgagcgc
     5401 cgcgcatgtg gaggccgagc cgcccggtga gccgcgcatg gacgacaggc cgacgccgtc
     5461 acccgccaag aagcgcgtca gccgtgaccc gccggcgcgc cggcgctcgc cggtcaccag
     5521 cgaggactcg gacgggccga tcacgcgtcc gactccgacc ttcgagaagc cgcggaaggc
     5581 ggatccctgg gagggtcgct tcgccgcggt catggccgag atcggggccg accgcgacgc
     5641 cgcggcgctg gcccaggcgc tggcgtggcg cgcgtcgagc cccggcgacg agatcgcact
     5701 gatcgcgctc ggcgaggcgg ccgaggcgag cggggacctc acgctggcgg cccgcgcgta
     5761 cggctcgctg atcgacctgt ttccggggcg cgccgacctg cggcgcatgg ccggcgcgca
     5821 cctcgaggcg ctcggcgagg tcggcctggc gctggcgatc gacacgtacc ggcaggcggt
     5881 cgcgcagcgg cccgaccacc ccgcgagcca ccgcctgctc gcgttcgcgt tgagcaaggc
     5941 cggccgccac gcggaggcgt tcgcggccgc gctcgcgggt gcgcagcgca gctacccccc
     6001 ggatcgcttc gcgggcgtgc aacggatctt gcgcgaggac ctcgcgctgc tggccgcggc
     6061 ctggcgcgcc gcacagccgg gcgacaaggc gatgctcgcc gcgctcgcgc aggccggggt
     6121 gaagcccgat acacggccct cgctgcgctt cgtgctcaac tgggagaccg acgccaacga
     6181 cgtcgatttt catgtctatg acggcaaggg cggccacgcg tactacgtag cgaagctgct
     6241 gccgggcgcc ggtgagctgt acgacgacgt gaggaccggc tacggcccgg agtgctttgc
     6301 gatcgcgggc gagcccgacg cgttcccgta ccagctgcag gcgcactact acagccgcgg
     6361 cccgatgggc tacggcatgg gcaagctgca ggcgatcgag cacgacggcg agggcaagct
     6421 ggtgttcaag gagcacccct tcgtcatcat gaaggaccgc gccttcgtcg acctcggccg
     6481 cgtcgacgcg ccgctggcga agctgcccgc ggaccagatc cgcctgccct cgggcgcggg
     6541 cgagggcaag gccccggtgg gcacgtacac gccgccgccg ccgccgatgc ccgcgcgctg
     6601 acctgtccgc aaggccaggt tcacggcgcc gtgacgacgg cgccggcgat gtcgaccgag
     6661 gtctgggcga a
//
LOCUS       DASWNJ010000470         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_185362, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000470 DASWNJ010000000
VERSION     DASWNJ010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185362"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="VGB85_08025"
     CDS             complement(<1..1266)
                     /locus_tag="VGB85_08025"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEY0134011.1"
                     /translation="MALVRPVRGLRYDLSRAGDAGLLLAPPYDVVDDAGRARIAARSP
                     HNCIHVILPQGEGDTKYAEGARQFERLKAEALTHDEAPSFYVYHQTFTSEGQQYTRKG
                     FIGLIELTRFGAGPVLPHERTLAGPKADRLKLMRACMAHLELVFGMFSDPARSWEASL
                     GELGQPVLDADFDGAHHTLHRVDDPQATAALAQLLADKKIYIADGHHRYETMCSFRDE
                     LAESRGETARWGLIYLSNLDDPGLVVLPTHRLVHSVPSFELRATLVALEPFFDITVTQ
                     LPGNAAELRTRLSESAGNGAAFGLAVPGAGELHVLALRKDFEPTAAGLGHLHAALQRL
                     DVALLHELVLERGLGITREAQSQNTNLRYYKSTDEALAVAVGGREGAADVQLVCFMNP
                     TPVADVRAVCDSGEVMPQKSTFFYPKIPTG"
     gene            complement(1313..>2206)
                     /gene="purD"
                     /locus_tag="VGB85_08030"
     CDS             complement(1313..>2206)
                     /gene="purD"
                     /locus_tag="VGB85_08030"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY0134012.1"
                     /translation="HFGREALREFATPPVVKASGLAAGKGVTVAETFADAELALRACL
                     EGQVFGGAGETVVLEERLTGEEASLFVISDGERWLCLPPAQDHKRLRDGDQGPNTGGM
                     GAYCPAPVCDARVLAAVEAEVIGPTLAGLRAEGRPFVGCLFVGLMIEGGRAKVIEYNA
                     RFGDPEIQPVLFGLQAPIVPHLYAAARGRLDPTASPLPAMPAATVVLAAEGYPGRVEH
                     GAAITGLAAASAIPDVKVFHAGTRRTADGWVTAGGRVLGVCARGPGLTEALARAYEAV
                     AAIEVAGGQHRRDIGARVRTR"
BASE COUNT          304 a          784 c          822 g          296 t
ORIGIN      
        1 acccgtcggg atcttcggat aaaagaacgt gctcttttgc ggcatgacct cgccgctgtc
       61 gcacacggcc cgcacgtccg cgaccggcgt cgggttcatg aagcagacca gctgcacgtc
      121 ggccgcgccc tcgcgaccgc cgacggcgac cgcgagcgcc tcgtcggtcg acttgtagta
      181 gcgcaggttg gtgttctgcg actgcgcctc gcgggtgatg ccgaggccgc gctcgagcac
      241 cagctcgtgc agcagcgcga cgtcgaggcg ctgcagcgcg gcgtgcaggt gaccgaggcc
      301 cgcggcggtc ggctcgaagt ccttgcgcag cgcgagcacg tgcagctcgc ccgcgccggg
      361 caccgcgagg ccgaaggccg cgccgttgcc cgcggactcc gacaggcggg tgcgcagctc
      421 ggccgcgttg ccgggcagct gcgtgacggt gatgtcgaag aagggctcga gcgccacgag
      481 cgtggcccgc agctcgaagc tcggcaccga gtgcacgagg cggtgggtcg gcagcacgac
      541 gaggccgggg tcgtcgaggt tgctgaggta gatcaggccc cagcgcgccg tctcgccgcg
      601 cgactcggcg agctcgtcgc ggaagctgca catcgtctcg tagcgatgat ggccgtcggc
      661 gatgtagatc ttcttgtcgg cgagcagctg cgcgagcgcg gcggtcgctt gcggatcgtc
      721 gacgcggtgg agcgtgtggt gggcgccgtc gaagtcggcg tcgagtaccg gctgtccgag
      781 ctcgccgagc gaggcctccc agctccgcgc cgggtcggag aacatgccga acacgagctc
      841 gaggtgggcc atgcacgcgc gcatcagctt gaggcggtcg gccttgggac ccgcgagcgt
      901 gcgctcgtgc ggcaggacgg gaccggcgcc gaagcgcgtc agctcgatca ggccgatgaa
      961 gcccttgcgc gtgtactgct ggccctcgct ggtaaaggtc tggtggtaca cgtagaagct
     1021 cggggcctcg tcgtgggtca gcgcctcggc cttgagccgc tcgaactgcc gcgcgccctc
     1081 ggcgtacttc gtgtcgccct cgccttgcgg caggatcacg tggatgcagt tgtgcggcga
     1141 gcgggcggcg atgcgggccc gaccggcgtc gtcgacgacg tcgtacggcg gcgcgagcag
     1201 cagacccgcg tccccggcgc ggctcaggtc gtagcgaaga ccacggacag ggcggacgag
     1261 tgccatcgcg tgccctcata ccacgccgga ccgcgcggcg gtcaagccgc ggtcatcgcg
     1321 tgcggacgcg ggcgccgatg tcgcggcgat gctgcccacc ggcgacctcg atcgcggcga
     1381 cggcctcgta ggcccgggcg agcgcctccg tgagccccgg gccgcgcgcg cagaccccga
     1441 ggacccgccc gcccgcggtg acccagccgt ccgcggtccg gcgcgtgccg gcgtggaaca
     1501 ccttcacgtc ggggatggcg gacgccgccg cgagccccgt gatggccgcg ccgtgctcga
     1561 cccggccggg atagccctcg gccgcgagca cgacggtcgc ggcgggcatc gccggcaacg
     1621 ggctcgcggt tgggtccagc cgaccacggg ccgccgcgta taggtgcggc acgatcggcg
     1681 cctgtaatcc gaacagcacc ggctggatct ccgggtcgcc gaagcgggcg ttatactcga
     1741 tcaccttcgc gcggccgccc tcgatcatca ggccgacgaa cagacagccg acgaagggcc
     1801 ggccctccgc gcgcaggccg gcgagggtcg ggccgatcac ctcggcctcg accgcggcga
     1861 gcacccgggc gtcacacacc ggcgccgggc agtaggcccc catgccgccg gtgttgggcc
     1921 cctgatcgcc gtcgcgcagg cgcttgtggt cctgcgcggg cggcaggcac agccagcgct
     1981 caccgtcgct gatcacgaac agcgaggcct cctcgccggt caagcgctcc tcgagcacga
     2041 cggtctcgcc ggcgccgccg aacacctggc cttcgagaca tgcgcgaagg gccagctcgg
     2101 cgtccgcgaa ggtctcggcg acggtgacgc ccttgccggc cgcgagcccg ctggccttga
     2161 cgaccggcgg ggtggcgaac tctcgcagag cctcacgccc gaagtg
//
LOCUS       DASWNJ010000471         1712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_256622, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000471 DASWNJ010000000
VERSION     DASWNJ010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256622"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1712)
                     /locus_tag="VGB85_08035"
     CDS             complement(<1..>1712)
                     /locus_tag="VGB85_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134013.1"
                     /translation="ADLLALASGECLRSAEALARAQRWAEFGEAPDDARAAIAAADAV
                     AGERETCSDLRVADGLLAFGDGAPELAAAALADARKFSSPSTLARHHHRWLAGMLALG
                     TGDAAAVAQAVHEADALCAEVPEAVAYHRLRARLQLQAGDPEAALASLAQVRAAARTH
                     LGLAADEALMNASLRREFSGVADLTDQLLGQNLAQNLGPPDRGHALLARAVVHVHAGE
                     AAAALARVDTAWPLLPWWDRNARTLALDLSLEAGDSQRALAWTAALAVPEPEAGVYRA
                     WALLVDGDIMAGLAALAQLPQEHPRVAYLQGLALVEQRRFEEAEPWLARADRLLPGRV
                     EIEVARARVAVHTGDHVMALRKLSGLAEEEVYAPRAYTGLGEAHLAQEPGKRDLRAAQ
                     KALSRAVEREPRAAEAMLLLAQVWQQRRAEAPEGTRNALEWYEQAAASNSRLPRYREA
                     LAAYLADLGQYARAEEMLRALVNETGITPETPLRLVDLVLGNHENAPLPDKVGREVQM
                     WIGTAEALGADAPALVRARARLDLALGTATTLRTAQSRLEQRLAQDPGDVESRILLVR
                     VRMA"
BASE COUNT          183 a          575 c          750 g          204 t
ORIGIN      
        1 ggccatgcgc acgcggacga gcaagatgcg gctctcgacg tcgccggggt cctgggcgag
       61 gcgctgctcg aggcggctct gggcggtgcg cagggtggtg gctgtcccga gggccaggtc
      121 gaggcgggcg cgggcacgga ccagcgcggg ggcgtcggcc ccgagcgcct cggcggtccc
      181 gatccacatc tggacctcgc ggccgacctt gtccgggagc ggggcgttct cgtggttgcc
      241 gagcacgagg tcgacgaggc gcagcggggt ctcgggggtg atgccggtct cgttgacgag
      301 cgcgcgcagc atctcctcgg cgcgcgcgta ctggccgagg tcggcgaggt aggcggcgag
      361 cgcctcgcga taccgcggca ggcgcgagtt gctggcggcg gcctgctcgt accactcgag
      421 cgcgttgcgg gtgccctcgg gggcctcggc gcggcgctgc tgccagacct gggcgagcag
      481 cagcatcgcc tcggcggccc tgggctcgcg ctcgacggcg cggctgaggg ccttctgggc
      541 ggcccgcagg tcgcgtttgc cgggctcctg cgcgaggtgg gcctcgccga ggccggtgta
      601 cgcgcgcggg gcatagacct cctcctcggc gagcccggag agcttgcgca gggccatcac
      661 gtggtcgccg gtgtgcacgg cgacgcgggc cctggcgact tcgatctcga cgcggccggg
      721 gagcaggcgg tcggcgcggg cgagccacgg ctcggcctcc tcgaagcggc gctgctcgac
      781 gagcgcgagg ccctgcagat aggcgacgcg cgggtgctcc tgcggcagct gggcgagcgc
      841 ggccaggccg gccatgatgt cgccgtcgac cagcaaggcc cacgcccggt acacaccggc
      901 ctccggctcg ggcacggcga gcgcggcggt ccacgcgagc gcgcgctgcg agtcacctgc
      961 ctcgagggac aggtcgagcg cgagcgtgcg ggcgttgcgg tcccaccacg gcagcagcgg
     1021 ccaggcggtg tcgacgcggg cgagcgcggc ggcggcctcg ccggcgtgca cgtggacgac
     1081 ggcgcgggcg agcaaggcgt ggccgcggtc gggcgggccg aggttctggg cgaggttctg
     1141 gccgagcagc tggtcggtga ggtcggcgac gccgctgaac tcgcggcgca gggaggcgtt
     1201 catcagggcc tcgtcggcgg cgaggccgag gtgggtgcgg gcggcggcgc ggacctgggc
     1261 gaggctggcg agggcggcct cggggtcgcc ggcttgcagc tgcaggcggg cgcgcaggcg
     1321 gtggtaggca acggcctcgg ggacctcggc gcacagcgcg tcggcctcgt ggacggcctg
     1381 ggcgacggcg gcggcgtcac ctgtcccgag ggccagcatg ccggcgagcc agcggtggtg
     1441 gtggcgggcg agcgtgctcg gcgagctgaa cttgcgggcg tcggcgaggg ccgcggcggc
     1501 gagctcgggg gcgccgtcgc cgaacgcgag caggccgtcg gcgacgcgca ggtcactgca
     1561 ggtctcgcgc tcgccggcga cggcgtcggc ggccgcgatc gcggcgcggg cgtcgtcggg
     1621 ggcctcgccg aactcggccc agcgctgggc ccgggccagc gcctcggcgg atcggaggca
     1681 ctcgccgctg gcgagcgcga gcaggtcggc gg
//
LOCUS       DASWNJ010000472         2952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_9007, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000472 DASWNJ010000000
VERSION     DASWNJ010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9007"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VGB85_08040"
     CDS             complement(<1..534)
                     /locus_tag="VGB85_08040"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0134014.1"
                     /translation="MLPDFNRLRVFFHVHQARSVSAAAAALHVTQSAISQSLAKLEDE
                     LGAQLFVRRHRAIVPTAAAEALFAVVEPFVSALHGGIEQIRRSRHALVGTLNLGAPVE
                     LGAHRLPAALAAFSRAHPEVSFTLRLGHPSELVPLLEEGKLDLAFVDLFDGPRPSARR
                     LAGLEVVEVMDEALVLVT"
     gene            605..1864
                     /gene="ahcY"
                     /locus_tag="VGB85_08045"
     CDS             605..1864
                     /gene="ahcY"
                     /locus_tag="VGB85_08045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY0134015.1"
                     /translation="MTTTSMDITDANGASRGRDRIEWAERAMPVLAAIRERFARERPL
                     AGVRISACLHVTAETAALVRTLAAGGASVRLCASNPLSTQDEVAASLAVHDGIAVFAR
                     AGEDRATYFQHLEAALAHRPQLVMDDGADLATLVHTTHAELGSEVIGGTEETTSGVSR
                     LRAMARDGVLRYPVIAVNDATTKHMFDNRHGTGQSTIDGFLRATNRLLAGACFVVCGY
                     GWCGRGLAQRAHGMGARVIVTEIDPLRALEATMDGYQVLPIAQAAAVGDFFCTVTGNH
                     SVITREHFLKMKDGAVVANAGHFDVEIDLAALRELSPARRIRPSLDEHTLPDGRRIYL
                     LAEGRLVNLAAAEGHPAAVMDLSFADQALSAEHVLAQRDLAVAVHAVPLLIDREVARL
                     KLRALGVEIDVETAAQRAYVDGWQSGT"
     gene            complement(1861..>2952)
                     /locus_tag="VGB85_08050"
     CDS             complement(1861..>2952)
                     /locus_tag="VGB85_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134016.1"
                     /translation="KIPPRHKIIAGTHLLNLGSTDLDSELRMALEIVHPRTVEVVVAP
                     FRLSYVDLHIPASQRSRHTSTCTFASDYEGAVGVPLDIKLYHVLPHFHYLGDSFSVDI
                     VGGPDDGKNLYELDGFDADANGRTFDPPVDLAGISGLRFSCGYDNWRDKEIGWGIGDQ
                     EMCVMLGLADSRAIMDMSVQNGSQVVGKDGEVLLNEGPCSVIALPKNAAQTLPTDAER
                     AGEFYIPPTGEADVDLDPEKRCVDSDPAAAPSGTTLSHLRGAVLTPGCIFSSCHDAKI
                     PVAGLDLQGPDLHAALLNHKPVADVDMPLVKPGDPDGSWLYQIVSRCEPRDSKGNAVR
                     PMPYNAPTLLPDEHVAALRAWISAGAKDD"
BASE COUNT          444 a         1030 c         1044 g          434 t
ORIGIN      
        1 ggtgacgagc accagcgcct cgtccatcac ctcgacgacc tcgaggccgg cgaggcgccg
       61 cgccgacggt cgtggtccgt cgaacagatc gacgaaggcg aggtcgagct tgccctcctc
      121 gagcagcggc accagctccg acgggtgccc gagccgcagc gtgaagctga cctctggatg
      181 cgcgcgcgag aaggcggcga gcgccgcagg tagtcgatgc gcgccgagct cgaccggcgc
      241 cccgaggttg agcgtcccga ccagcgcgtg ccggctgcga cggatctgct cgatgccgcc
      301 gtgcaacgcg gacacgaacg gctcgaccac ggcgaacagc gcctcggcgg ccgccgtcgg
      361 cacgatcgcc cggtgccgcc gaacgaacag ctgcgcgccg agctcgtcct cgagcttcgc
      421 caggctctga ctgatcgccg attgcgtgac gtgcagcgcc gctgcggccg cgctgacgct
      481 gcgagcctgg tgcacatgga aaaatacgcg caagcggttg aagtcgggca gcataagcag
      541 tgctaatatg acatgataaa tcttagaatg tgactaatcc atggcctcga cgcatcgtct
      601 ggtcatgacg acgacatcga tggacatcac ggatgcaaac ggggcctcgc gcgggcgtga
      661 caggatcgag tgggccgagc gggcgatgcc cgtgctcgct gcgatccgcg agcgcttcgc
      721 ccgcgaacgg ccgctcgccg gcgtgcggat ctccgcctgc ctgcacgtca ccgccgagac
      781 cgcggcgctg gtgcgcaccc tcgcggccgg cggcgccagc gtgcgcctgt gcgccagcaa
      841 cccgctatcg acgcaggacg aggtcgcggc ctcactggca gtacacgacg gcatcgccgt
      901 gttcgcgcgc gccggcgagg accgggccac ctacttccag cacctcgagg ccgcgctcgc
      961 ccaccgcccg cagctggtga tggacgacgg cgccgacctc gcgaccctcg tgcacacgac
     1021 ccacgccgag ctcggcagcg aggtcatcgg cggcaccgag gagacgacct ccggcgtcag
     1081 ccgcctgcgc gcgatggccc gcgacggcgt gttgcgctac cccgtgatcg ccgtcaacga
     1141 cgcgaccacc aagcacatgt tcgacaaccg ccacggcacc ggccagagca cgatcgacgg
     1201 cttcctgcgc gcgaccaacc gcctgctcgc cggcgcctgc ttcgtcgtct gcggctacgg
     1261 ctggtgcggc cgcggcctcg cccagcgcgc ccatgggatg ggcgcccgcg tcatcgtcac
     1321 ggagatcgac ccgctgcgcg ccctcgaggc gacgatggac ggctaccagg tcctcccgat
     1381 cgcccaggcc gccgcggtcg gcgacttctt ctgcaccgtc accggcaacc acagcgtcat
     1441 cacccgcgaa catttcttaa aaatgaaaga cggggcggtg gtcgcgaacg cggggcactt
     1501 cgacgtcgag atcgacctcg cggccctgcg cgagctgtcg ccggctcgca ggatccggcc
     1561 gtcgctcgac gagcacacgc tcccggacgg gcgccggatc tacctgctcg ccgagggccg
     1621 cctcgtcaac ctcgccgccg ccgaggggca tccggcggcg gtgatggacc tcagcttcgc
     1681 cgaccaggcc ctctcggccg agcacgtgct cgcgcagcgc gacctcgccg tcgccgtgca
     1741 cgcagtacct ttgctcatcg accgcgaggt cgcgcgcctc aagttgcgcg cgctcggggt
     1801 cgagatcgac gtggagaccg ctgcgcagcg ggcctacgtc gacggctggc agagcgggac
     1861 ctagtcgtcc ttggcgcccg cggagatcca ggcccgcagc gcggcgacgt gctcgtccgg
     1921 cagcagggtc ggcgcgttgt agggcatcgg ccgcaccgcg ttgcccttgc tgtcgcgcgg
     1981 ctcgcagcgc gacacgatct gatacagcca gctgccgtcg gggtcgccgg gcttgaccag
     2041 cggcatgtcg acatcggcga ccggcttgtg attgagcagc gcggcgtgca ggtcggggcc
     2101 ttgcaagtcg agccccgcga ccgggatctt cgcgtcgtgg cacgacgaga agatgcagcc
     2161 gggcgtcaac acggccccgc gcaggtggga cagcgtcgtg ccgctcgggg cggccgcggg
     2221 gtcgctgtcg acgcagcgct tctcggggtc gaggtcgacg tccgcctcgc cggtcggcgg
     2281 gatgtaaaac tcgcccgcgc gctcggcgtc ggtcggcagg gtctgggcgg cgttcttcgg
     2341 cagggcgatg acgctacacg ggccctcgtt gagcaggacc tcgccgtcct tgccgaccac
     2401 ctgcgagccg ttttgcaccg acatgtccat gatggcgcgg ctgtcggcga ggccgagcat
     2461 gacgcacatc tcctggtcgc cgatgcccca accgatctcc ttgtcgcgcc agttgtcgta
     2521 gccgcacgag aagcgcaggc cgctgatgcc ggcgaggtcg accggcgggt cgaaggtgcg
     2581 gccgttggcg tcggcgtcga agccgtcgag ctcgtaaagg ttcttgccgt cgtcggggcc
     2641 gccgacgatg tcgacgctga agctgtcgcc gaggtagtgg aagtgcggca gcacgtgata
     2701 gagcttgatg tcgagcggga ccccgacggc gccctcgtag tcgctggcga acgtgcacgt
     2761 gctggtgtgg cggctgcgct ggctcgcggg gatgtgcagg tcgacgtagc tgaggcgaaa
     2821 cggcgcgacc acgacctcga ccgtgcgcgg gtgaacgatc tcgagggcca tgcgcagctc
     2881 gctgtcgagg tcggtgctgc cgaggttgag caggtgcgtg ccggcgatga tcttgtggcg
     2941 cggggggatc tt
//
LOCUS       DASWNJ010000473         3911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_291502, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000473 DASWNJ010000000
VERSION     DASWNJ010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291502"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VGB85_08055"
     CDS             complement(<1..336)
                     /locus_tag="VGB85_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134017.1"
                     /translation="MAPAPAAPVAAATPAYGEAQADSDLETVLSARGKEYLEARARLE
                     ASAAVAAPRVAARLRRVPAPSAAEERRLLALLAGMARPEDLEMFATQLRRDVAASHQR
                     SPGERDELRA"
     gene            complement(425..1813)
                     /locus_tag="VGB85_08060"
     CDS             complement(425..1813)
                     /locus_tag="VGB85_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134018.1"
                     /translation="MFASRLRAALVVTGMLCAPAVVDAAREPAPRRGESSAQQLHRRG
                     VHCMEEIERPECAIEHFEALLAERTEDRELITDGLLRLIKLYEKAGDDDAVKSVMRRF
                     WEAGRGQIRRGHLPYTTRFLPGDFDMLAVANMQRMLQAPLAKRLPDEMREFILTCDAD
                     RRRQLEDLRLYRRAQSRAQAKQLTTQQALDELTAEERKAAEKRRQRAAQRDTQRPKRS
                     KPVFADGMCATALALGDTSVEAWKRVALAFHHDDWTRSAAVVEIPGLAARIADGVQAG
                     RLVAAGPNTWTVVGRTYEGGALVIASYELDELVIAPAKLLPQIAASHGRGGRSLAKEV
                     DKLVAGVPVDAGFFAVATEKAVRELGFSGMSKGRRKLFEMLLPRPKGVQVAGVVHEYF
                     GMFIRMPTDTPVKAGMLVDIARRLIENQGEGDAEAADNLRLLDVAQATDRRALLLGYV
                     LTPGQIEQMLLE"
     gene            1919..2965
                     /gene="queA"
                     /locus_tag="VGB85_08065"
     CDS             1919..2965
                     /gene="queA"
                     /locus_tag="VGB85_08065"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF001140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEY0134019.1"
                     /translation="MPGPEDFDFELPADQIAQEPAARRGDARMLVLAAPGSEPPWQHS
                     EARRLPDFLPKDALVVVNDSRVVPARVFLGAANGRRFELLVCDPRPGLGPGRRFKAWV
                     RGAKHLKTGARLEHDAFACTLIGPDDIDPRARVFEVEAGDLLTTLHAAGQVPLPPYIR
                     REAGPDARDSERYQTLYAAAPGSVAAPTAGLHWEPEVLARLDLVRITLHVGPGTFLPM
                     EAADVREHRVGAEHAEISDEAADRVRAARRQGRPIIAIGTTATRALEAVAATHGELRP
                     HSGPVDLVITPDHRFRVVDGLVTNFHLPRSSLLMLVCSLGGRERVLAAYREAVRAGYR
                     FYSYGDCMLLAPAV"
     gene            3016..>3911
                     /locus_tag="VGB85_08070"
     CDS             3016..>3911
                     /locus_tag="VGB85_08070"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEY0134020.1"
                     /translation="MRTDVLEIPRVEPRAGLSCENAAIDFRPPGSFEVLVQDGPARAG
                     RLWTAHGPVDTPCFMPVGTYGAVKGLDPDDLRECGSQIILGNSYHLGHRPGSARVAGL
                     GGLHQMMSWDRPILTDSGGFQVFSLRGLQKLDDAGVDYQTHFDGSRHRLTPESVLRTQ
                     AALGSDICMILDHCPPGDATPVQMRAAVDRSTRWARQAIDLRAGILAPHQLCFGIVQG
                     GTDLALRREHLAELAALPFDGLALGGLSVGEPIPEMHATLAEIGPEMPRERPRYVMGI
                     GTPFDLLAAVSAGILAPHQLCFG"
BASE COUNT          492 a         1594 c         1289 g          536 t
ORIGIN      
        1 cgcgcgcagc tcgtcgcgct cgcccggcga tcgctggtgg ctcgcggcga cgtcgcggcg
       61 cagctgcgtg gcgaacatct cgaggtcctc cggtcgcgcc atcccggcga gcagcgccag
      121 cagtcggcgc tcctccgcgg ccgacggcgc cggcacccga cgcaggcgcg cggcgacccg
      181 cggggccgcg acggcggcgc tggcctcgag gcgcgcgcgg gcctcgaggt actccttgcc
      241 acgcgcgctc aggaccgtct cgaggtcgct gtccgcctgc gcctcgccgt acgcgggcgt
      301 cgcggcggcc acgggtgcgg cgggtgcggg cgccacggcg ggcgcgagga gggagccgat
      361 cagggcgcag gacagaaagc acgacacgcg gccaaggctg ccacagcgcc gggtccgcag
      421 cgagtcactc gagcagcatc tgctcgatct gccccggcgt cagcacgtag ccgagcagca
      481 gcgcccgccg gtcggtcgcc tgggccacgt cgagcaggcg caggttgtcg gcggcctcgg
      541 cgtcgccctc gccctggttt tcgatcagtc gccgggcgat gtcgacgagc atgcccgcct
      601 tcaccggcgt gtcggtcggc atgcggatga acatcccgaa gtactcgtgg accacgccgg
      661 cgacctgcac gcccttgggt cgcggcagca gcatctcgaa cagcttgcgc cggcccttcg
      721 acatccccga aaagccgagc tcgcgcaccg ccttctcggt ggcgaccgcg aagaagccgg
      781 cgtcgaccgg cacgccggcg acgagcttgt cgacctcctt cgccaggctc ctgccgccac
      841 gcccgtggct cgcggcgatc tgcggcagca acttcgccgg cgcgatcacc agctcgtcga
      901 gctcgtagct cgcgatcacc agcgccccac cttcatacgt gcgcccgacg accgtccacg
      961 tgttcggccc cgccgcgacc aggcggccgg cctgcacgcc gtcggcgatc cgggccgcca
     1021 gccccgggat ctccacgacc gccgccgagc gcgtccagtc gtcgtggtga aacgccagcg
     1081 cgacccgctt ccacgcctcc acgctggtgt cgccgagcgc cagcgccgtc gcgcacattc
     1141 cgtcggcgaa caccggcttc gaccttttgg gccgctgcgt gtcgcgctgc gccgcccgct
     1201 gcctgcgctt ctccgccgcc ttgcgctcct ccgccgtcag ctcgtcgagc gcctgctggg
     1261 tcgtcagctg tttggcctgg gcccggctct gcgcgcggcg atacaggcgc aggtcctcga
     1321 gctggcgccg gcggtcggcg tcgcaggtca ggatgaattc tcgcatctcg tccggcagcc
     1381 gcttcgccag cggcgcctgc aacatccgct gcatgttggc gaccgcgagc atgtcgaagt
     1441 cgccgggcaa aaagcgcgtc gtgtagggca ggtgaccgcg gcggatctgg ccgcgcccgg
     1501 cctcccagaa ccgccgcatc accgacttca ccgcgtcgtc gtcgccggct ttctcataca
     1561 gcttgatcag ccgcagcagc ccgtcggtga tcagctcacg gtcctcggtg cgctcggcga
     1621 gcagcgcctc gaagtgttcg atcgcgcact ccggccgctc gatctcctcc atgcagtgca
     1681 ccccgcggcg gtgcagctgc tgggccgagc tctcaccgcg ccgcggcgcc ggctccctcg
     1741 ccgcgtcgac caccgccggc gcacacagca tccccgtcac gacgagggca gcgcgcaggc
     1801 gggaggcgaa caccggcgca gcatgacacc ggccgccatc aacggccaac ctcggcccac
     1861 tccggcccca gggcgcccct tgtgtcgctc tcgggcctgt gccatggggg agccgccgat
     1921 gcccggaccc gaggacttcg acttcgaatt gcccgccgac cagatcgccc aggagccggc
     1981 ggcgcggcgc ggggatgcgc gcatgctcgt gctcgcggcc cccggcagcg agccgccgtg
     2041 gcagcacagc gaggcccgcc gcctgccgga cttcctcccc aaggatgccc tcgtcgtcgt
     2101 caacgactcg cgcgtcgtcc cggcgcgggt gttcctcggc gccgcgaacg gccgccgctt
     2161 cgagctcctc gtctgcgacc cgcgccccgg cctcggcccc ggccgtcgct tcaaggcgtg
     2221 ggtccgcggc gccaagcacc tcaagacggg agcccgcctc gagcacgacg ccttcgcctg
     2281 cacgctgatc ggccccgacg acatcgatcc gcgggcccgc gtcttcgagg tcgaggccgg
     2341 cgacctgctg accacgctgc acgccgcggg gcaggtcccg ctgccgccgt acatccgccg
     2401 cgaggccggc cccgacgccc gcgacagcga gcgctaccag accctctacg ccgccgcgcc
     2461 cggctcggtc gccgccccga ccgccggcct gcactgggag cccgaggtcc tcgcccgcct
     2521 cgacctcgtc cggatcaccc tgcacgtcgg ccccggcacc ttcttgccga tggaggccgc
     2581 cgatgtccgc gagcaccgcg tcggcgccga gcatgccgag atctccgacg aagccgccga
     2641 ccgcgtccgt gcggcgcgcc gccagggccg gccgatcata gcgatcggca ccaccgccac
     2701 gcgcgcgctc gaggccgtcg cggccaccca cggcgagctg cgcccgcaca gcggccccgt
     2761 cgacctcgtc atcacccccg accaccgctt tcgcgtcgtc gacggcctcg tcaccaactt
     2821 ccacctgccg cgcagctccc tgctgatgct cgtgtgcagc ctcggcggcc gcgagcgcgt
     2881 cctggccgcc taccgcgagg ccgtgcgcgc cggctaccgc ttctactcct acggtgactg
     2941 catgctgctc gcgcccgcgg tctgaaccgg ccgcccttgt ctcgagcggg acccctgtgg
     3001 catgcctcgc cgcgcatgcg caccgacgtc ctcgagatcc cccgcgtgga gccccgcgcc
     3061 ggcctgagct gtgagaacgc cgccatcgac ttccggccgc ccggcagctt cgaggtgctc
     3121 gtgcaggacg gtccggcccg cgccggccgt ctgtggaccg cccacggccc ggtcgacacc
     3181 ccctgcttta tgccggtcgg cacctacggg gcggtcaagg gcctcgaccc ggacgacctg
     3241 cgcgagtgcg gctcgcagat catcctcggc aacagctacc acctcggcca tcggccgggc
     3301 tcggcaaggg ttgccggact cggcggcctg caccagatga tgtcctggga ccggccgatc
     3361 ctgaccgaca gcggcggctt tcaggtgttc agcctgcgtg gcctgcaaaa gctcgacgac
     3421 gccggcgtcg actaccagac ccacttcgac ggctcgcgcc accgcctgac ccccgagagc
     3481 gtgctgcgga cccaggccgc gctcggctcc gacatctgca tgatcctcga ccactgcccc
     3541 ccgggcgacg cgaccccggt gcagatgcgc gcggccgtcg accgcagcac ccgctgggcc
     3601 cgccaggcga tcgacctgcg cgccggcatc ctcgctccgc accagctgtg cttcggcatc
     3661 gtccagggcg gcaccgacct cgcgctgcgc cgtgagcacc tcgccgagct cgccgcgctg
     3721 cccttcgacg gcctcgccct cggcggcctc agcgtcggcg agccgatccc cgagatgcac
     3781 gcgaccctcg ccgagatcgg ccccgagatg ccgcgcgaac ggccgcgcta cgtcatgggg
     3841 atcggcacgc ccttcgacct gctcgccgcc gtgagcgccg gcatcctcgc tccgcaccag
     3901 ctgtgcttcg g
//
LOCUS       DASWNJ010000474         6105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_44211, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000474 DASWNJ010000000
VERSION     DASWNJ010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44211"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VGB85_08075"
     CDS             <1..700
                     /locus_tag="VGB85_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134021.1"
                     /translation="DRAPEWARLRLFGHAIPAMRLYRGEEPRGYINGRELYGRGAVIL
                     VWELPWLLAHTREFWAELPGFYAQLQRHDPALMAAGVDTYFVFPSVDESKARRPMADP
                     EINNAEIGTDTLRFAINAETRDHIRSEIPPGTTDLIPLFLLESASDDPNNLGLEHPAS
                     DIREWLFDRIYAPVILVIDHRGIVRWHSRDFPIGPEETIEAAVQFALKQLPEAPASTA
                     APAAPESAAPVSGP"
     gene            complement(687..983)
                     /locus_tag="VGB85_08080"
     CDS             complement(687..983)
                     /locus_tag="VGB85_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134022.1"
                     /translation="MTARARSQRRVTGGNPAVAVLRTSGRVARRPTPSLDQAVQKTPD
                     AGVLASAEQDASQGAFLMKRLASPWALCRLFRLFMVGALGYAVYEVVAAVLMAR"
     gene            complement(1000..1803)
                     /locus_tag="VGB85_08085"
     CDS             complement(1000..1803)
                     /locus_tag="VGB85_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEY0134023.1"
                     /translation="MGLFSRLGTLIRSNINELINKAEDPEKMLNQVLVDMKQQLIEAK
                     KQVAVAIADEKRIKKQWEQEATKAAEWEKKAMLAIRAGDDTLARAALARKADHDEVAN
                     QLKDQWEAQKTSVENLKGALRGLDNKIEEAKRKRNLLVSRQKRAEAQRTINETLSSIN
                     STSAFDTFDRMSDRVTQLEAEAEASQEISGALTEGSLESQFKALEASSVDDQLDALKR
                     KMALTAGKTEDPRQIGSGKPAAAEPPVDEMKAAEIDAQLEELKKKLSGP"
     gene            complement(1807..2241)
                     /locus_tag="VGB85_08090"
     CDS             complement(1807..2241)
                     /locus_tag="VGB85_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEY0134024.1"
                     /translation="MSYAETLESYMLRMELPHERVSEDTWVLAPDSLRHARIVACVDE
                     PIVLYSTPLFAVSELTPDREGLFRKLLEFNDHLLYCAYALDNEQIVLSGAQQILDLDY
                     GEFQGMIEDMVMALDSHLDQLTPWRPATVQHVQPVQPDHEGT"
     gene            complement(2479..2715)
                     /locus_tag="VGB85_08095"
     CDS             complement(2479..2715)
                     /locus_tag="VGB85_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134025.1"
                     /translation="MPTCRGKFATAIAPEPAAPAAPPAGAAGAVPPAPEPDSFTVTGA
                     PPEARKDQSTDQSTAQSAKQWQSTTAARPETRTA"
     gene            2696..3457
                     /gene="uppS"
                     /locus_tag="VGB85_08100"
     CDS             2696..3457
                     /gene="uppS"
                     /locus_tag="VGB85_08100"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEY0134026.1"
                     /translation="MPRHVGIIMDGNGRWARQKGLPREVGHEHGADAVRRVVRAARAL
                     GIEALTLFAFSSQNWDRPPQEVFHLMQLLNRYLLEERAEILDNGIRLITVGDTDRLPG
                     YVRDPMRELITASAHHKDMTLCLALSYGGREMITEAARRLCRLVAAGKLRPEDVDAKA
                     FEAQLDSSKMLPPLDLLIRTSGEHRISNFFLWEVAYAELFFSEAMWPDFAREHLEEAL
                     QDYSRRERRFGTVPEAEASDEPAYPGLFPPQRGKG"
     gene            3461..4288
                     /locus_tag="VGB85_08105"
     CDS             3461..4288
                     /locus_tag="VGB85_08105"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY0134027.1"
                     /translation="MGDFVRRLWVAVILVPILVIGLFVDPTPWSILVMSAIATMFAHD
                     EFLRMALPVRQEDAEWPLRLVVVGLLGGAIHSLPMISGTQVLPPLLSISVMVVALTML
                     MRRHHLTSAGRHMAACVASLLYVPLLASVWPLMKADFGAGWLFMTLCLAFFSDTVAYI
                     FGRLWGRHKLYPAVSPGKTLEGSVGGLVGGMLATVVAGSLLLVPDLSLGHAVVLGIVG
                     SLFGQVGDLFESMLKRGYGVKDSSSLLGAHGGMLDRVDALLFVAPIVYYYARFVVYA"
     gene            complement(4307..4552)
                     /locus_tag="VGB85_08110"
     CDS             complement(4307..4552)
                     /locus_tag="VGB85_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134028.1"
                     /translation="MLLAMIGFGVAGATFAYAKKQDASNGTAAAAATATGVGTAVVGT
                     VVVAASSVLVPVAVVGGIGYWFLKRGGKNKALGSGRS"
     gene            4800..5153
                     /locus_tag="VGB85_08115"
     CDS             4800..5153
                     /locus_tag="VGB85_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134029.1"
                     /translation="MARVPATVVRVQATAARVREIVVRVPADRVPVIAAREAMARVPA
                     TVARVPTLGGRVPMARVVMGRVVMARVTTARVTTARVTTGRVTTARVATTAVRGVTIA
                     ARVVTTTAATTPPRR"
     gene            5174..>6105
                     /locus_tag="VGB85_08120"
     CDS             5174..>6105
                     /locus_tag="VGB85_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134030.1"
                     /translation="MQDKQVPCRIAGCENTWTWFAAQQIRGFGQPPPDRMCDEHLGQF
                     NELADREVPCRTPGCPNTWTWKRGAQLAEMQRTGKLRRPSRLCAECFTAERETHDTEL
                     PCKIDGCKRTWTWTRDAQLRHRLWVLRQRAKADAVAARGARSRDEQGEAREGEVREGE
                     APRAREGREGEPRSRNRGEAREGEVREGEPRSRNRGEAREGEARSRNRGEARGGEARE
                     GEAQQGEAREGEAPRAREGEGRSRNREPREGEAPRSRNREPREPRELREGDAPRLRTR
                     EQTHEEPGGFDVTVERALPFLGSEPVAGSDVAVSG"
BASE COUNT          822 a         2086 c         2263 g          934 t
ORIGIN      
        1 cgaccgcgcg ccggagtggg cccgcctgcg cctgttcggc cacgcgatcc cggcgatgcg
       61 gctgtatcgc ggcgaggagc cgcgcggcta catcaacggg cgcgagctgt acggccgcgg
      121 cgctgtgatc ctcgtgtggg agttgccgtg gctgctggca cacacgcgcg agttctgggc
      181 cgagctgccc ggcttctatg cccagctcca gcgccacgac ccggcgctga tggccgcggg
      241 cgtcgacacg tacttcgtgt tcccctcggt cgacgagtcg aaggcgcgcc ggccgatggc
      301 tgacccggag atcaacaacg cggagatcgg cacggacacg ctgcgcttcg cgatcaacgc
      361 ggagacgcgc gatcacatcc gcagcgagat cccgcccggc acgaccgacc tcatcccgct
      421 gttcttgctc gagtccgcgt cggacgaccc gaacaacctc ggcctcgagc acccggcgtc
      481 ggacatccgc gagtggctgt tcgaccgcat ctacgcgccg gtgatcctcg tgatcgacca
      541 ccgtgggatc gtccgctggc atagccgcga cttcccgatc ggccccgagg agacgatcga
      601 ggccgcggtg cagttcgcgt tgaagcagct cccggaggcg ccggcgagta cggctgcacc
      661 ggctgcaccg gagtcggctg cgcccgtcag cgggccatga gcaccgcggc gacgacctcg
      721 tagacggcgt agccgagggc cccgaccatg aacaggcgga acaggcggca cagggcccac
      781 gggctcgcca ggcgcttcat caggaaggcg ccctggctcg cgtcctgttc ggcgctggcg
      841 agcacgccgg cgtccggggt cttctggacc gcctggtcga gagacggggt cggccggcgc
      901 gcgacccgtc cgctggtacg cagtacggcg acggcaggat tgccgcccgt gacacgacgt
      961 tgagatcggg cgcgggcggt catgatggtg cagggcggct caggggcccg agagcttctt
     1021 cttgagctct tcgagctggg cgtcgatctc ggcggccttc atctcgtcga ccggcggctc
     1081 ggcagcggcg ggcttgccgg agccgatctg acgcgggtcc tcggtcttgc cggcggtgag
     1141 cgccatcttg cgcttgaggg cgtcgagctg gtcgtcgacg ctgctggcct cgagggcctt
     1201 gaactgcgac tcgagcgagc cctcggtcag cgcgccgctg atctcctggc tggcctcggc
     1261 ctcggcctcg agctgcgtga cgcggtccga catgcggtcg aaggtgtcga aggcgctggt
     1321 gctgttgatg ctcgacaggg tctcgttgat cgtgcgctgg gcctcggccc gcttctggcg
     1381 cgagaccagc aggttacgct tgcgcttggc ctcctcgatc ttgttgtcga ggccgcgcag
     1441 ggcgcccttg aggttctcga ccgaggtctt ttgcgcctcc cactggtcct tgagctggtt
     1501 ggcgacctcg tcgtggtcgg ccttgcgggc gagcgcggcg cgggcgagcg tgtcgtcgcc
     1561 ggcgcggatc gccagcatcg cctttttctc ccactcggcg gccttggtcg cctcctgctc
     1621 ccactgcttc ttgatgcgct tctcgtcggc gatggcgacg gcgacctgct ttttggcctc
     1681 gatcagctgc tgcttcatgt ccaccaggac ctgattgagc atcttctcgg ggtcttcggc
     1741 cttgttgatg agctcgttga tgttcgagcg gatgagggtg ccgagtcgtg aaaaaagtcc
     1801 catgatttag gtgccctcgt gatcaggctg gacaggctgg acatgctgga cggtcgcggg
     1861 ccgccaaggg gtgagctggt cgaggtggct gtcgagcgcc atcaccatgt cctcgatcat
     1921 cccctgaaat tcgccgtagt cgaggtcgag gatctgctgc gcaccggaca gcacgatctg
     1981 ctcgttgtcg agggcgtagg cgcagtagag gaggtggtcg ttgaactcga gcagcttgcg
     2041 gaacaggccc tcgcggtccg gggtcagctc ggacaccgcg aacagcggcg tcgagtagag
     2101 gacgatcggc tcgtcgacgc aggcgacgat gcgcgcgtgg cggaggctgt ccggggcgag
     2161 cacccaggtg tcctccgaca cccgctcatg ggggagctcc atccggagca tgtacgattc
     2221 gagggtctcg gcgtaactca tgggcggggg cggtccgagg gggaaggtcc cggcagcggg
     2281 cgcaggtgcc tgtgcgcggc cgcgagccgg gagctcgggg gggagattct atgcgatcgg
     2341 ccagcagggc ggcaacctcc accccgcgca agtgcgccca aagtgctttc acggggggcc
     2401 tgcgccgtgg tatcccgctg gaccccggga gacacgccgc cgccatgcag ggatcgccag
     2461 gtttcgccgc cacgcacttc atgccgtcct cgtctccggg cgtgctgccg tcgtgctctg
     2521 ccactgcttc gcgctctggg ccgtgctctg gtccgtgctc tggtccttgc gggcctcggg
     2581 gggggcgccc gtgaccgtga acgagtcggg ctccggcgct ggtgggacgg ctccggccgc
     2641 gccagccggt ggggccgccg gggccgccgg ttcaggggcg atcgccgtcg cgaacttgcc
     2701 ccggcacgtc ggcatcatca tggacggtaa cggccggtgg gcgcggcaaa agggcctgcc
     2761 gcgcgaggtc gggcacgagc acggcgcgga cgcggtgcgt cgggtcgtcc gggccgcgcg
     2821 cgcgctcggc atcgaggcgc tgacgctgtt cgcgttctcg agccagaact gggaccgccc
     2881 gccgcaggag gtcttccacc tcatgcagct gctcaaccgc tacctgctcg aggagcgggc
     2941 ggagatcctc gacaacggca tccgcctgat caccgtcggc gacaccgacc gcctgccggg
     3001 ctacgtgcga gatcccatgc gcgagctgat caccgcgtcg gcgcaccaca aggacatgac
     3061 cctgtgcctc gcgctcagct acggcggccg cgagatgatc accgaggccg cgcggcggct
     3121 gtgccggctg gtcgccgccg gcaagctgcg ccccgaggac gtcgacgcca aggcgttcga
     3181 ggcccagctc gactcgagca agatgctgcc gccgctcgac ctcctgatcc ggaccagcgg
     3241 cgagcacagg atctccaact ttttcctgtg ggaggtcgcc tacgccgagc tgttcttcag
     3301 cgaggcgatg tggcccgact tcgcgcgcga gcacctggag gaggccctgc aggactacag
     3361 ccggcgcgag cgacggttcg gcacggtgcc ggaggccgag gcctcggacg agccggccta
     3421 cccggggctg tttccgccgc agcggggcaa gggctgacgc atgggagact tcgtccggcg
     3481 gctgtgggtc gcggtcattt tggtcccgat cctcgtcatc gggctgttcg tcgacccgac
     3541 gccgtggagc atcctggtga tgtcggcgat cgcgacgatg ttcgcgcacg acgagttttt
     3601 gcgcatggcg ctgccggtgc gccaggagga cgccgagtgg ccgctgcggc tggtggtcgt
     3661 cggcctgctc ggcggcgcga tccacagctt gccgatgatc tccgggacgc aggtgctgcc
     3721 gccgctgctg tcgatctccg tgatggtcgt cgcgctgacg atgttgatgc gccggcacca
     3781 cctcacgagc gccggccggc acatggccgc gtgcgtcgcc agcctgctct acgtgccgtt
     3841 gctggccagc gtgtggccgc tgatgaaggc cgacttcggg gcaggctggc tgttcatgac
     3901 gctgtgcctg gcgttcttct ccgacacggt cgcgtacatc tttggccgcc tgtggggccg
     3961 ccacaagctg tacccggcgg tcagccccgg caagaccctg gagggctcgg tcggcggcct
     4021 ggtcggcggc atgctggcga cggtggtcgc gggctcgctg ctgctggtgc ctgacctgtc
     4081 gctcggtcat gcggtggtgc tggggatcgt cggctcgctg ttcgggcagg tcggcgacct
     4141 gttcgagtcg atgctcaagc gcggctacgg ggtcaaggac tcgagctcgc tgctcggggc
     4201 ccacggcggg atgctcgacc gggtcgacgc gctgctgttc gtcgccccga tcgtctacta
     4261 ctacgcccgc ttcgtggtgt acgcgtagga ccggcgaggg ccgcgctcag gagcggccgg
     4321 agccgagcgc cttatttttg ccgccgcgct tgagaaacca gtagccgatc cccccgacga
     4381 cggcgacggg cacgagcacg ctgctggcgg cgaccaccac ggtgccgacc acggccgtcc
     4441 cgacgcccgt ggcggtggca gcagccgcgg cggtgccgtt ggaggcgtcc tgcttcttgg
     4501 cataggcgaa ggtggcgcca gcgactccga aaccgatcat ggccaacaac atgcttgaac
     4561 cctccgaggc gcgagaaatc gcggaggcca cagtatagac cggcgggcgg gcgcacgctc
     4621 gtgcggactg gacggggggg gcccggggac tgatacttgc gccgtggcag aggaccccaa
     4681 gggcaattca ggcgagccgt ccgcgcggac ggacgggcca gaggcacaga gcggcgagcg
     4741 gaccggtccg gagcgcgctc ctcgggagcg cagcgacggc gcggcgaacc gtggcgccga
     4801 tggcccgcgt cccggcgacc gtggtccgcg tccaggcgac cgcggcccgc gtccgggaga
     4861 tcgtggtgcg cgtcccggcg gaccgcgtcc cggtgatcgc ggcccgcgaa gcgatggccc
     4921 gcgtcccggc gaccgtggcc cgcgtcccga cgctcggggg ccgcgtcccg atggcccgcg
     4981 tagtgatggg ccgcgtagtg atggcccgcg taacgacggc ccgcgtaacg acggcccgcg
     5041 taacgacggg ccgcgtaacg acggcccgcg tcgcgacgac cgcggtccgc ggcgtgacga
     5101 tcgcggcccg cgtcgtgacg acgaccgccg caacgacacc gcctcgccgc tgacggcctc
     5161 gctgttcagc gccgtgcagg acaagcaggt gccgtgccgg atcgccggct gcgagaacac
     5221 gtggacgtgg ttcgccgcgc agcagatccg cggcttcggc cagccgccgc cggaccgcat
     5281 gtgcgacgag cacctcggcc agttcaacga gctcgccgac cgcgaggtgc cgtgccgtac
     5341 gcccggctgc ccgaacacct ggacgtggaa gcgcggtgcc cagctcgccg agatgcagcg
     5401 caccggcaag ctgcgccggc cgtcgcgcct gtgcgccgag tgcttcaccg ccgagcgcga
     5461 gacccacgac accgagctgc cgtgcaagat cgacggctgc aagcggacct ggacctggac
     5521 ccgtgacgcc cagctgcgcc accgcctgtg ggtcctgcgc cagcgcgcca aggccgacgc
     5581 cgtggcggcc cgtggcgccc gctcgcgtga cgagcaaggc gaggcccgcg agggcgaggt
     5641 ccgcgagggc gaggcacccc gcgcccgcga aggtcgtgag ggcgagccgc gctcgcgtaa
     5701 ccgcggcgag gcccgcgagg gtgaggtccg cgagggcgag ccgcgctcgc gtaaccgcgg
     5761 cgaggcgcgc gagggtgagg cacgctcgcg caaccgcggc gaggcccgcg ggggtgaggc
     5821 ccgcgagggc gaggctcaac agggcgaggc ccgcgagggc gaggctccgc gcgcccgcga
     5881 gggcgagggg cgctcgcgca accgcgagcc ccgcgagggc gaggctccgc gctcgcgcaa
     5941 tcgtgagccc cgcgagcccc gcgagctgcg cgagggtgac gcgccgcgcc tgcgcacgcg
     6001 cgagcagacc cacgaggagc cgggcggctt cgacgtcacg gtcgagcggg cgctgccctt
     6061 cctcggtagc gagccggtgg ccgggtccga cgtcgctgtc tccgg
//
LOCUS       DASWNJ010000475         3351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_291528, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000475 DASWNJ010000000
VERSION     DASWNJ010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291528"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VGB85_08125"
     CDS             <1..399
                     /locus_tag="VGB85_08125"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134031.1"
                     /translation="ILGAIEVERDPADLRQISAMVCPPALSRSRRHGIDNLATHAVAA
                     LGLTRGVARVDLILNERHNEVILEVEPLPPLHRDGVVAKVALAAGLRYEQLVAALIHD
                     MVEAPQRRPAPRVSVEQALGGAALGSAVLQ"
     gene            431..970
                     /locus_tag="VGB85_08130"
     CDS             431..970
                     /locus_tag="VGB85_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134032.1"
                     /translation="MADHPFSFIGAGEASYFEWADLHIYFERAPKPAERTAILKEVPP
                     PLADSADWDGKHLYVASEQGVGRVIEAAYANPPKQPSRLTTTSRFKIAAPSRTTRFNA
                     DIERWLHAAHAIVPILAAYRRPDDEAGGTKLSSWHAESVRRVPAIVKAIGKVKPGAAV
                     KAMIAGMKAELAAARAAPD"
     gene            complement(1010..1651)
                     /locus_tag="VGB85_08135"
     CDS             complement(1010..1651)
                     /locus_tag="VGB85_08135"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF domain-containing protein"
                     /protein_id="HEY0134033.1"
                     /translation="MAESLAGQLKREREETRALLADGYAQDHIDQDELERRLDLVETA
                     ATVEELRALTTELRPMETALVPVRAATQERIPVTLGSLERAGAWQVSSRTTVRVLFGS
                     AELDLREAVLPPGDIEIEVKVVFGNLELIVPPGWQIDNRCGAVLGSIEQDPSSPVSGP
                     RRVLRLTGRVVFSSLAVYERLPGEGSFGAWRRRRGEKKALAERSARALRRGDE"
     gene            complement(1707..2441)
                     /locus_tag="VGB85_08140"
     CDS             complement(1707..2441)
                     /locus_tag="VGB85_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0134034.1"
                     /translation="MRYAIFSDIHANHDALEAVLRAVDREKVDALVCLGDTVGYGAQP
                     NECCDIVRRVTRYTILGNHDAAVAGRMDYSYYYDAAREALDWHAGQLTQENMGWLRKL
                     NYFEKDGMTHFCHGSPVDLEEFEYIFSVEQAQTCIPIWESLGQITFIGHSHLCKSFAI
                     GKERVYEVVTKEFEIHPEFRYIVSVGSVGQPRDHDPRASYTIYDDAKGRFEFKRVDYD
                     VNAAARKIFSDQRLSDSFGARLFAGV"
     gene            complement(2441..>3351)
                     /locus_tag="VGB85_08145"
     CDS             complement(2441..>3351)
                     /locus_tag="VGB85_08145"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY0134035.1"
                     /translation="SPGAEPPRFEARPEPVRSEPAELPRPAEMPRPAGLPRPRRSNQP
                     AVAVSGEIGGGLARPSATARTSELRPEARAESSGGDPKQRVAKVIEGLAGKRPADVLP
                     ALAELDEDELLAVLPPLVEVAGRLVPELLTRLESPRRPLRQAAAIVLGIARDRRAIDP
                     LTALLGSEPTPVWVDVARALGNFGPRVVGPLCALLRGAAPESKEGLGQRVARTFAELV
                     VSDGDAPGGAGRLAVENLLEVADPTVSSAARRALATLADVRGEARGTGGGADDREIRR
                     FSTRAYEAITTPELDADGDIEVVGDD"
BASE COUNT          486 a         1257 c         1127 g          481 t
ORIGIN      
        1 atcctcggcg cgatcgaggt cgagcgcgac cccgccgacc tgcgccagat ctccgcgatg
       61 gtctgcccgc ccgccctgtc gcgcagccgc cggcacggca tcgacaacct cgccacccac
      121 gcggtcgccg ccctcggcct cacccgcggc gtcgcccgcg tcgacctcat cctcaacgag
      181 cgccacaacg aggtcatcct cgaggtcgaa cccctccccc cgctccaccg cgacggcgtc
      241 gtcgccaagg tcgccctcgc cgccggcctg cgctacgagc agctcgtcgc ggccctgatc
      301 cacgacatgg tcgaggcacc ccagcgccgc ccggcccccc gcgtgtccgt cgagcaggcg
      361 ctcggcggcg ccgcgctcgg ctcggccgtg cttcagtagt cgcgcccggt cacgcggaga
      421 ggatcggagg atggcggacc atccgttttc cttcatcggc gccggcgagg cgagctactt
      481 cgagtgggcc gacctccata tctacttcga gcgcgcgccg aaaccagcgg agcggacggc
      541 gatcctcaag gaagtgccgc cgccgctcgc tgactcggcg gactgggacg gcaagcatct
      601 ctatgtcgcg agcgagcagg gtgtcgggcg cgtgatcgag gctgcgtacg cgaacccgcc
      661 gaagcagccg tcgaggttga cgacgacgag tcggttcaag atcgcagcgc cctcccgtac
      721 gacgcggttc aacgcggaca tcgagcgctg gctacacgcg gcccacgcga tcgtgccgat
      781 cctcgcggcc taccggcgac cggacgacga ggcgggtggg acgaagctgt cgagctggca
      841 cgccgagagc gtgcgtcgcg tgcccgcgat cgtgaaggcg atcggcaagg tgaagccagg
      901 cgccgccgtg aaagcgatga tcgcaggcat gaaggccgag ctcgcagcgg cgcgcgccgc
      961 cccggactga accgccacgc gcgtcactgg acacacatac acgagcgcgt tactcgtccc
     1021 cgcgtcgcag cgcccgggcc gagcgctcgg cgagcgcctt cttctcgcct cgccgccgcc
     1081 gccaggctcc gaagctgccc tcgccgggca ggcgctcgta caccgccagg ctgctgaaga
     1141 ccacgcgtcc ggtcagtcgc agcacgcgtc gcgggccgct gaccgggctg ctcgggtctt
     1201 gctcgatcga cccgaggacc gcgccgcagc ggttgtcgat ctgccagccc ggcgggacga
     1261 tcagctcgag gttgccgaac accaccttga cctcgatctc gatgtcaccc gggggcagca
     1321 ccgcctcgcg caggtcgagc tccgccgatc cgaacagcac gcgcaccgtc gtccgcgacg
     1381 acacctgcca cgcgccggcc cgctcgagcg acccgagcgt caccgggatg cgctcctgcg
     1441 tcgccgccct caccggcacc agcgccgtct ccatcggccg cagctcggtg gtcagggcgc
     1501 gcagctcctc gaccgtggct gccgtttcga ccaggtcgag gcgacgctcg agctcgtcct
     1561 ggtcgatgtg gtcctgcgcg tagccgtcgg cgagcagcgc ccgcgtctcc tcgcgttcac
     1621 gcttgagctg ccctgccagg gactcggcca tggcccgatc taccacgccc ctatccgaga
     1681 agtggccgcg aaatcggccg cgccggtcac acgccggcga acaggcgcgc gccgaagctg
     1741 tcggaaaggc gctggtcgct gaagatcttg cgggcggcgg cgttgacgtc gtagtcgacg
     1801 cgcttgaact cgaagcggcc cttggcgtcg tcgtagatcg tgtagctcgc gcgcgggtcg
     1861 tggtcgcggg gctggccgac cgagccgacc gacacgatgt agcggaactc cgggtggatc
     1921 tcgaactcct tcgtcaccac ctcgtagacg cgctccttgc cgatcgcgaa cgacttgcac
     1981 aggtgcgagt ggccgatgaa cgtgatctgg ccgaggctct cccagatcgg gatgcaggtc
     2041 tgggcctgct cgacgctgaa gatgtactcg aactcctcga ggtcgaccgg cgagccgtga
     2101 cagaagtggg tcatcccatc cttctcgaag tagttgagct tgcgcagcca gcccatgttc
     2161 tcctgggtca gctggccggc gtgccagtcg agcgcctcgc gggcggcgtc gtagtagtag
     2221 ctgtagtcca tgcgaccggc gacggccgcg tcgtggttgc cgaggatcgt gtaccgcgtg
     2281 acgcggcgga cgatgtcaca gcactcgttg ggctgcgccc cgtagccgac cgtatcgccg
     2341 aggcagacga gcgcgtccac cttctcgcgg tcgaccgcac ggagcacggc ctcgagggcg
     2401 tcatggttgg catgaatatc gctgaaaatt gcgtaacgca tcagtcgtcc cccaccactt
     2461 cgatatcgcc gtcggcgtcg agctccggcg tcgtgatggc ctcgtaggct cgcgtggaga
     2521 accgacggat ctcacgatcg tcggcgccac cacccgtccc cctcgcctcg cctcgcacgt
     2581 ccgccagtgt agcaagggca cgccttgccg cggaggaaac cgtcgggtcg gcgacctcga
     2641 ggagattttc gaccgcgagc cgaccggcgc cgccgggcgc gtctccgtcc gagaccacga
     2701 gctcggcgaa cgtccgcgcc acgcgctgcc cgagcccctc cttggattcc ggcgccgcgc
     2761 cgcggagcag cgcgcacagc ggcccgacca cgcgcggccc gaagttgccg agcgccctgg
     2821 cgacgtcgac ccacaccggc gtcggctccg agccgagcag cgcagtcagc ggatcgatcg
     2881 cccggcggtc cctggcgatg cccagcacga tcgcagctgc ctgacgcagc ggccgccgcg
     2941 gggactcgag gcgggtgagc agctccggga ccaggcgccc cgccacctcg accagcggcg
     3001 gcaacacggc cagcagctcg tcctcatcga gctcggcgag cgccgggagg acgtctgccg
     3061 gtcgctttcc cgcgagccct tcgatcacct tcgccacccg ctgcttgggg tcgccaccgc
     3121 tcgattccgc gcgcgcctcg ggccggagct ccgaggtgcg agccgtcgcg ctcggccggg
     3181 ccagcccgcc gccaatttcg ccggagaccg ccaccgcggg ttgattactg cgacgcggcc
     3241 gcggcagccc ggccggccgc ggcatctcgg ccggccgtgg cagctcggcc ggctcactgc
     3301 gcaccggctc gggccgggcc tcgaacctcg gcggttcggc gccggggctc g
//
LOCUS       DASWNJ010000476         2617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_395547, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000476 DASWNJ010000000
VERSION     DASWNJ010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395547"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VGB85_08150"
     CDS             complement(<1..186)
                     /locus_tag="VGB85_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134036.1"
                     /translation="MTYLDRILAVAVAGVCALPGCAGDDKGDDNGFVVMPGTSVTSVT
                     TVTTTPDGSSSESSGGDE"
     gene            433..1155
                     /locus_tag="VGB85_08155"
     CDS             433..1155
                     /locus_tag="VGB85_08155"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0134037.1"
                     /translation="MAHEHAHSTHPIVPGFGADRAAHYDAQAPISLAGFDAMYELGAS
                     ALATRLDGQDTASLLFVGLGTAAELLPYRRFAVPRWHFTGVDPSAAMLEVARERLTAE
                     GLLARSHLHVGELRTLPAGPAFDGAQMLGVLHHVPEQHERVELLREVTRRLKPGAPLV
                     LGCRVGTDATVTDIEMRRMRAYGFPADALERRREAMKTVRPIESEAALFAMLAETGLV
                     TPHLIFASLAFKVFLVRFAPAG"
     gene            1259..>2617
                     /locus_tag="VGB85_08160"
     CDS             1259..>2617
                     /locus_tag="VGB85_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134038.1"
                     /translation="MPDRSWPLAGVALLFVACGPPAAPGQTPPAPGPALASEPVASDR
                     SGEAVPKDSQPPPPPPISAPTPPVPAPPALPSISPPSAGPPLAPQVLTASSPGGPITL
                     HLAAIREGRIVLEPLVDGTLALRGDVAFAIAAPGASPRREPAWMRGIPREPYEPLEPL
                     VFGGRWPDAAFMSFSQELSRSGDGYPMMRWQGDRWTDIERPEEALGLASYHAAYAGGP
                     GGAVLGLRSHVLDTFSDDDFTPFMIRKIKAALRSVRPSVDRLDAAEPTAWPALPPGPV
                     GIGLLSFPDGTLVVMRSGPKLQRWNPGATAWKTLPAVGYTATGYYDGPTLVGRDPARL
                     YMFSCPDATQPRLHRLVGAAWESIATPDGKCVWSLAEDAGGAVWLITSGGLYRHVAPG
                     PPPGAVPSIAWESITLPAVQLPAQPGAWRHDNILSGWHMFPEVAAEQQALIASRGGAL
                     GPD"
BASE COUNT          332 a          980 c          935 g          370 t
ORIGIN      
        1 ctcgtccccg ccgctcgact ccgagctcga cccgtccggg gtcgtggtca cggtggtcac
       61 ggacgtcacc gacgtccccg gcatcaccac gaagccgttg tcgtcgccct tgtcgtcccc
      121 ggcgcaaccc gggagcgcgc agacgcctgc cactgccact gcgaggatcc gatcgaggta
      181 agtcataagt ttcgggcata gcaaagacag tcccggcgtc gagttttttg gcgagcgaac
      241 cgacgtgctg gcagttttgc ccggggatcg tcgcgcttga atatttcctg ggtggtgaaa
      301 tctcggatcg gtccgcatcg atggaggggg acgcggaccg cgggccggtg gcgcgatcga
      361 aactttggcc gatcgggcac aaatcctcgc cctgtcgccc ggccgcgtgg tgacggatcc
      421 tcgcggacac ccatggccca cgagcacgcc cactccaccc acccgatcgt gccaggcttc
      481 ggcgccgacc gggccgccca ctacgacgcc caggccccga tctcgctcgc cggcttcgac
      541 gcgatgtacg agctcggcgc cagcgcgctc gccactcgac tcgacggcca ggacaccgcg
      601 tcgctgctgt tcgtcgggct cggcaccgcc gcggagctgc tgccgtaccg gcgcttcgcg
      661 gtcccgcggt ggcacttcac cggcgtcgac ccgtcggcgg cgatgctcga ggtcgcccgc
      721 gagcgcctga ccgcggaggg gctgctcgcg cgctcgcacc tgcacgtcgg cgagctgcgc
      781 acgctgcccg ccgggcccgc cttcgacggc gcgcagatgc tgggcgtgct gcaccacgtg
      841 cccgagcagc acgagcgcgt cgagctgctg cgcgaggtga cgcggaggtt gaagcccgga
      901 gcgccgctcg tgctcggctg ccgcgtcggc acggacgcga cggtgaccga catcgagatg
      961 cggcgcatgc gagcgtacgg gttcccggcg gacgcgctcg agcgccggcg cgaggcgatg
     1021 aagacggtgc gcccgatcga gtcggaggcg gcgctgttcg ccatgctcgc cgagaccggg
     1081 ctggtgacgc cgcacctgat cttcgcgtcc ctggccttca aggtgttcct cgtccgcttc
     1141 gcgccggccg gctgagtcgc ggcgcgcgcg accgagcgtc gatgcgcgcg acccgaaagc
     1201 cgcgcgacga tggacgtcga ggacgcggct gcccgggttc ctgggctacc ctctccagat
     1261 gcccgaccgc tcctggccgc tcgctggcgt cgcgctgctg ttcgtcgcct gcggccctcc
     1321 tgccgcgccc gggcagaccc caccggcacc gggccccgcg ctggcgagcg agcctgtcgc
     1381 ttcggacagg tccggggaag ctgtcccaaa ggacagccag cctccgccgc cgccgccgat
     1441 ctccgccccc acgccgccag tgccggcccc tccggccctg ccgtcgatat cgccgccgag
     1501 cgcaggacca ccgctggcgc cccaggtcct gacggcgagc agcccgggcg gaccgatcac
     1561 cctgcacctc gcggcgatcc gcgaggggcg gatcgtgctc gagccgctcg tcgatggcac
     1621 gctcgccctg cgcggcgacg tcgcgttcgc gatcgcggcg ccaggcgcct caccgcgccg
     1681 cgagccggcg tggatgcgcg ggatcccccg cgagccctac gagccgctcg agccgctggt
     1741 gttcggcggg cgctggcccg acgcggcctt catgagcttc tcgcaggagc tctcgcgctc
     1801 gggcgacggc tatccgatga tgcgctggca gggcgaccgc tggaccgaca tcgagcggcc
     1861 cgaggaggcg ctcgggctcg ccagctacca cgccgcctac gcgggtggcc cggggggagc
     1921 ggtgctcggg ctgcgcagcc acgtgctcga cacgttcagc gatgacgact tcaccccttt
     1981 tatgatccgc aagatcaagg ccgcgctgcg cagcgtgcgt ccgagcgtcg accggctcga
     2041 cgccgcggag ccgaccgcct ggccggcgct gccgcctggc ccggtcggca tcggcctgct
     2101 gagcttcccc gacggcacgc tcgtggtgat gcgcagcggc ccgaagctgc agcgctggaa
     2161 cccgggcgcg accgcgtgga agacgttgcc cgccgtcggc tacacggcga ccggatacta
     2221 cgacggcccg acgctggtcg gccgcgaccc ggcgcgcctg tacatgttct cgtgccccga
     2281 cgcgacgcag ccgcgcctgc accgactggt gggagcggcc tgggagtcga tcgccacgcc
     2341 cgacggcaag tgcgtgtggt cgctggccga ggacgccggg ggcgcggtgt ggctgatcac
     2401 ctccgggggc ctgtatcgtc acgtggcccc gggcccgccg ccgggcgcgg tgccgtcgat
     2461 cgcgtgggag tcgatcacgc taccggccgt gcagctgccg gcgcagcccg gggcgtggcg
     2521 ccacgacaat atcctcagcg ggtggcacat gttcccggag gtcgcggcgg agcaacaggc
     2581 gctgattgcc tcgcgcgggg gggcgctcgg acccgac
//
LOCUS       DASWNJ010000477         2483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_185480, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000477 DASWNJ010000000
VERSION     DASWNJ010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185480"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1342
                     /locus_tag="VGB85_08165"
     CDS             <1..1342
                     /locus_tag="VGB85_08165"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0134039.1"
                     /translation="ENEALYRLSTRVNEAAEPEELLAALVDETGSPDVQGKIQAIETA
                     PSGQPEAVEVIAVHGGGPTVGSPVGARFDLRGATSVQLWTARPNEALVVEDLANDPRL
                     DPAARAAHTANGLGAVILLPLRWHDRWLGLLQLGWAHARKIGDDQRRLFERIAPQAAA
                     VLDNRLLLARSQRAIAENQAQAHTLEIVLDHLPVGVVIIDATNGQRRINRAGADLLAD
                     DEPDVREPLPLYHPDSDQPVAPQERLSRLVLAHGEVVSRERDLLGHDGTRRRLTVTAA
                     PFRDEHGAILGTITLFHDITQQVRGEREREQLKEAVIAAQRAALAERATPLIPISAEI
                     LVLPLVGTIDPERGEQLVQTLGDLDGHACTRVVLVDLTGVRDLDGPGALALARAAGVL
                     RLRGVTAIFTGIGDTVAWTLVDQNIDLRGLTTHATLRSGLAHAARLTGAADLAR"
     gene            complement(1445..>2483)
                     /locus_tag="VGB85_08170"
     CDS             complement(1445..>2483)
                     /locus_tag="VGB85_08170"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY0134040.1"
                     /translation="RRPGVVRAVAWNVERGKRFAALRHTLVNDPLLREADILLLTEVD
                     IGMGRSQNLNVPRELAAALGMGYVFANYHIVLAEGDRGERGHGVPNTKAMHGCVLLTR
                     FPVLRFEAVELRERRDKFHATEKRMGNKRALLCELLLPDGPLSIALVHLDPFTGPRHR
                     AKQLRQIIRRLRAFGGARMLLGGDLNTNTYDLGSSQGLVVNLLYKFTRFGFNGTIEQY
                     MKPEQLVERKVFAALRVGGLAIEGYNDFARGTIYYDVYDPEMARKALDYLPQPVVRWM
                     MRWLERRLAPWDGRVPLRLDWFAGAGLRPRSPQVIERPTVDGRLISDHNPILVDLELA
                     PAVDSGPGGSG"
BASE COUNT          367 a          977 c          813 g          326 t
ORIGIN      
        1 cgagaacgag gcgctctacc ggctctcgac ccgggtcaac gaggccgccg aacccgaaga
       61 gctgctcgcc gcgctcgtcg acgagaccgg atcccccgac gtccagggca agatccaggc
      121 gatcgagaca gctccctcgg gacagccgga ggccgtcgag gtcatcgccg tgcacggcgg
      181 cggcccgacc gtcggatccc ccgtcggcgc ccgcttcgat ctgcgcggcg cgacgagcgt
      241 ccagctgtgg accgcgcgcc cgaacgaggc gctcgtcgtc gaggacctcg ccaacgaccc
      301 ccgcctcgac cccgccgcgc gcgcggccca caccgccaac ggcctcggcg ccgtcatcct
      361 cctgccgctg cgctggcacg accgctggct cggcctgctg cagctcggct gggcccacgc
      421 gcgaaagatc ggcgacgacc agcgccgcct gttcgagcgt atcgccccgc aggccgccgc
      481 ggtgctcgac aaccgcctgc tcctcgcgcg cagccagcgg gcgatcgccg agaaccaggc
      541 ccaggcgcac accctcgaga tcgtgctcga ccacctcccg gtcggcgtgg tgatcatcga
      601 cgccacgaac ggccagcgca ggatcaatcg cgccggcgcc gacctgctcg ccgacgacga
      661 gcccgacgtc cgcgagccgc tcccgctcta ccaccccgac agcgatcagc ccgtcgcgcc
      721 ccaggagcgg ctgtctcgcc tcgtcctcgc ccacggcgag gtcgtcagcc gtgagcgcga
      781 cctgctcggc cacgacggca cgcgacgccg cctcaccgtc accgccgccc cgttccgcga
      841 cgagcacggc gcgatcctcg ggacgatcac gctgttccac gacatcaccc agcaggtccg
      901 cggcgaacgc gagcgcgagc agctcaagga ggccgtcatc gccgcccagc gagccgccct
      961 cgccgagcgc gcgacccccc tgatcccgat ctccgccgag atcctcgtcc tcccgctcgt
     1021 cggtacgatc gaccccgagc gcggcgaaca gctggtccag accctcggcg acctcgacgg
     1081 ccacgcctgc acccgcgtcg tcctcgtcga cctcaccggc gtccgcgacc tcgacggccc
     1141 cggcgccctc gccctcgccc gcgccgccgg cgtcctgcgc ctgcgcggcg tcactgcgat
     1201 cttcaccggc atcggcgaca ccgtcgcctg gaccctcgtc gatcaaaaca tcgacctgcg
     1261 cggcctcacc acccacgcaa ccctccgctc cggcctcgcc cacgccgccc gactgaccgg
     1321 cgctgccgac ctcgcccggt gagccgctcc tcgccgcgga tgcaccgagc gtcgcgggtt
     1381 cgggagacgc tcctcgccgc aggcgcactg gaccctggcg tgttcgagaa ccgagccgac
     1441 gatcctagcc agatccaccc gggccggagt cgaccgcggg agccagctcg agatcgacga
     1501 ggatcggatt atggtcgctg atcagccgcc cgtcgacggt gggtcgttcg atgacttgcg
     1561 gcgagcgagg ccgcagcccc gcgccggcga accagtcgag gcgcagcggc acgcggccgt
     1621 cccagggcgc gaggcggcgc tcgagccagc gcatcatcca tcggacgacc ggctgcggca
     1681 ggtagtcgag cgccttgcgg gccatctccg ggtcgtagac gtcgtagtag atcgtgccgc
     1741 gggcgaagtc attataaccc tcgatcgcca ggccgccgac gcgcagcgcc gcgaacacct
     1801 tgcgttcgac gagctgctcc ggcttcatgt actgctcgat cgtgccgttg aagccgaagc
     1861 gcgtgaactt gtagagcagg ttgacgacga ggccctggct gctgccgagg tcgtaggtgt
     1921 tggtgttgag gtcaccgccg agcagcatgc gcgcgccgcc gaaggcgcgc aggcggcgga
     1981 tgatctgccg cagctgcttg gcgcggtggc gcgggccagt gaacgggtcg aggtggacca
     2041 gcgcgatcga cagcggaccg tccgggagca gcagctcgca cagcagcgcg cgcttgttgc
     2101 ccatccgctt ctccgtcgcg tggaacttgt cccggcgctc gcgcagctcg accgcctcga
     2161 agcgcagcac cgggaagcgc gtcagcagca cgcagccgtg catcgccttg gtgttgggca
     2221 ccccgtgccc gcgctcgccg cggtcgccct cggcgagcac gatgtgatag ttggcgaaca
     2281 cgtagcccat gccgagcgcg gcggccagct cgcgcggcac gttgaggttt tgcgagcggc
     2341 ccatgccgat gtcgacctcg gtcaacagca agatgtcggc ctcgcgcagc agcgggtcgt
     2401 tgaccagcgt gtgacgcagc gccgcgaagc gcttgcctcg ctcgacgttc cacgcgaccg
     2461 cgcgcaccac ccccgggcgt cgc
//
LOCUS       DASWNJ010000478         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_114356, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000478 DASWNJ010000000
VERSION     DASWNJ010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114356"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2006
                     /locus_tag="VGB85_08175"
     CDS             <1..>2006
                     /locus_tag="VGB85_08175"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY0134041.1"
                     /translation="LSADDRGAAQWLRGLEDLRAQQYSSAAERFAEARKAPALASVAD
                     RLRVREAQAWLDAAEPLRALKAIDDLAEGTGNIAAAMLITADARARGDDPKAAAALYD
                     RYLQRSGESGKRHEARAKLARLLAVSADQEDQRKALALYERLALDVPLSDYGEEATQR
                     IPALEQALGIRKSFAENREAVRQRSLARMRDALARSRYANVVRDADEFLGLAAVTDGD
                     RCEALFLKGSAVFKQRKRAKSRPVFEQSAAACRQAGAKYQDTLVKALYQAGRGRYAEA
                     TYESAARQFEAIAEQFGGHSYADDALILAGESWAEHDDHPRERLAYERILKDIPGGDM
                     VGEARRRLIVLAFVQDRVADAQSLAEAGLAAAPGPKSDLRERAKLHYFRGRALQRQGQ
                     TEAAVAAWLDVLAAGPMTYASLQALSRLRDVGDEAFQKGVAVLERPQGSAAVELELPA
                     TPAAKRAQLLARLGLGEEAGEELSEAGVAGWPAVAVLAQAGLYAESQRLLGGLGSAWR
                     VSPPAGPIKNLWQIAHPMAFGEIVRADEAAHDVPPFLAFAIMQTESRFDPGATSWAGA
                     RGLIQLMPATAKDLAKQAGLADFNPENLYQPSLNLDLGTRYLGNLVRRFGGDDAAVPL
                     AIPSYNAGAGAVDKWLERRKSWDFDLFIEAIPFDETRAYTQSVL"
BASE COUNT          291 a          813 c          661 g          241 t
ORIGIN      
        1 ctctccgcgg acgaccgcgg cgccgcgcag tggctgcgtg gcctcgagga cctgcgcgcc
       61 cagcaatact cgtcggccgc cgagcgcttc gccgaggccc gcaaggcgcc cgcgctcgcc
      121 agcgtcgccg accgcctgcg cgtgcgcgag gcccaggcct ggctcgacgc cgccgaacca
      181 ctgcgcgccc tcaaggcgat cgacgacctg gccgaaggga caggcaacat cgccgccgcg
      241 atgctgatca ccgccgacgc cagggcccgc ggcgacgacc cgaaagccgc cgccgcgctc
      301 tacgaccgct acctccagcg cagcggcgag tccggcaagc gtcacgaggc gcgcgccaag
      361 ctggcccgcc tgctcgcggt ctcggcggat caggaggacc agcgcaaggc gctcgcgctg
      421 tacgagcgcc tcgccctcga cgtcccgctc agtgactacg gcgaggaggc cacccagcgc
      481 atcccggcgc tcgagcaggc cctggggatc cgcaagtcct tcgccgagaa ccgtgaggcc
      541 gtgcgccagc gcagcctcgc ccgcatgcgc gacgcgctcg cgcgcagccg ctacgccaac
      601 gtcgtgcgcg acgccgacga gttcctcggc ctggccgccg tgaccgacgg cgaccgctgc
      661 gaggccctgt ttttgaaggg cagcgctgtc ttcaaacaac gcaagcgcgc caagtcgcgg
      721 ccggtcttcg agcagtcggc cgcggcctgc cggcaagccg gcgcgaagta ccaggacacg
      781 ctcgtcaagg cgctctacca ggccggccgc ggccgctacg ccgaggcgac ctacgagtcc
      841 gccgccaggc agttcgaggc gatcgccgag cagttcggcg gccactccta cgccgacgac
      901 gccctgatcc tcgccggtga gtcgtgggcc gagcacgacg accacccgcg cgagcgcctc
      961 gcctacgaac gtattttaaa agacatcccc ggcggcgaca tggtcggcga ggcccgccgt
     1021 cgcctgatcg tcctcgcgtt cgtgcaggac cgcgtcgccg acgcccagag cctcgccgag
     1081 gccggcctcg ccgccgcacc gggccccaag tccgacctgc gcgagcgcgc caagctgcac
     1141 tacttccgcg gccgcgccct ccagcgccag ggccagaccg aggccgccgt cgccgcctgg
     1201 ctcgacgtgc tcgccgccgg cccgatgacc tacgcctccc tgcaagcgct gtcccgcctg
     1261 cgcgacgtcg gcgacgaggc gtttcaaaag ggtgtcgccg tgctcgagcg cccgcagggc
     1321 agcgccgcgg tcgagctcga gttgcccgcc acaccagcgg cgaagcgagc ccagctgctc
     1381 gcgcgcctcg ggctcggcga ggaggccggc gaggagctct cggaggcggg cgtcgccggc
     1441 tggcccgcgg tcgccgtgct cgcgcaggcg ggcctgtacg ccgagtcgca gcgcctgctc
     1501 ggcggcctcg gcagcgcctg gcgcgtcagc ccgccggccg gcccgatcaa aaacctgtgg
     1561 cagatcgccc acccgatggc cttcggcgag atcgtgcgcg ccgacgaggc cgcccacgac
     1621 gtcccgccct tcctcgcctt tgcgatcatg cagaccgaga gccgcttcga ccccggcgcg
     1681 acctcctggg ccggcgcccg cggcctgatc cagctcatgc ccgcgaccgc caaggacctc
     1741 gcgaagcagg ccggcctcgc cgacttcaac cccgagaacc tctaccagcc ctcgctcaac
     1801 ctcgacctcg gcacccgcta cctcggcaac ctcgtgcgcc gcttcggggg tgacgacgcc
     1861 gccgtcccgc tcgcgatccc gagctacaac gccggcgccg gcgcggtcga caagtggctc
     1921 gagcgccgca agtcgtggga cttcgacctc ttcatcgaag cgatcccctt cgacgagacc
     1981 cgcgcctaca cccagtcggt gctcga
//
LOCUS       DASWNJ010000479         2544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_256684, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000479 DASWNJ010000000
VERSION     DASWNJ010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256684"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VGB85_08180"
     CDS             <1..318
                     /locus_tag="VGB85_08180"
                     /inference="COORDINATES: protein motif:HMM:NF026048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HEY0134042.1"
                     /translation="YSEWGNLDLNYETIAKIDPLKCIGCHNCVVACHDGAHQCIHPDP
                     GGKTRVPVVDESECVGCNLCQIVCPVPGCISMVEVPNGMAPATWNDHVADGAPLRAKK
                     GVH"
     gene            641..1816
                     /locus_tag="VGB85_08185"
     CDS             641..1816
                     /locus_tag="VGB85_08185"
                     /inference="COORDINATES: protein motif:HMM:NF022995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HEY0134043.1"
                     /translation="MAMVAMPSQRLLDELMRYIEFTPEDAAQMYAMGPVLRPHFARVV
                     DRFYEAIDRTPAAAAVFTGGAEQIERQKTLLHGWLEGLVGGVYDDAYLARRARIGRTH
                     VRIGLDQRYMFSGMNIVRSELHNALYDSTEWPADQQRSGHRAIDRICDIELAIMLETY
                     REAYIARVKEAERLATIGQVAASIGHELRNPLAVMKTSVHMLTRRVPSDERTVKHLYR
                     LDEQIVLCNTIISDLLELARDRPAERIATDIATVVSEAALSVPRVASIGLGLHIEPKI
                     PSVAVDPGQFRQLVINLVLNATQVLEGSGRIEVRLGADEQNVVLTVEDSGPGITPDVQ
                     ARLFEPLFTTRSSGTGLGLALCRRVVEKHNGSIVASNREGGGAAFVVRVPLAWEAES"
     gene            1813..2184
                     /locus_tag="VGB85_08190"
     CDS             1813..2184
                     /locus_tag="VGB85_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0134044.1"
                     /translation="MSLEALVVDDNRQLAENLAEILAEEGCRVRMAFSAEEALGAAEA
                     VCPDFVLTDIRLPGMNGVELIRRLVVRAPEATFLLMTAYTSDQILRDASHLGVVRAVL
                     PKPLAIDRLLALLPRRPGRAM"
     gene            2181..>2544
                     /locus_tag="VGB85_08195"
     CDS             2181..>2544
                     /locus_tag="VGB85_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0134045.1"
                     /translation="MSGRVLIVEDNVALAENIAELFADLEVEAVRCATGAEALAAADP
                     NIDLAIVDVRLPDITGVELLPRLRPRMPDAEVILMTGDASLATAIAAVREGVFAYVQK
                     PFAPQDLLALGLRAQAQAQ"
BASE COUNT          379 a          864 c          926 g          375 t
ORIGIN      
        1 tactcggagt ggggtaacct cgatctcaac tacgagacca tcgccaagat cgacccgctc
       61 aagtgcatcg gctgccacaa ctgcgtggtc gcttgccatg acggcgcgca tcagtgcatc
      121 caccccgatc cgggcggcaa gacgcgcgtg ccggtggtcg acgagagcga gtgcgtcggg
      181 tgcaacctgt gccagatcgt ctgcccggtg ccggggtgca tctcgatggt cgaggtgccc
      241 aacggaatgg cgccggcgac ctggaacgat cacgtcgcgg acggggctcc gctgcgggcc
      301 aaaaagggcg tgcactgagg acgggctcgt gcgggcttcc gggctcgcgc gggctgtgtc
      361 cgatgaaggc ccggggcgag gctgactcgc cgggctccgc tcgtggtcgg gccgacgcgg
      421 tcgcatttta ggcggacggc cggccgcgag tcgacatcgt gtcgcgggat cgaatgtgac
      481 gcgtcaatgg cccggcgcac acgaccgggc gaggcgagcg cgcaaacttg gggccgggcg
      541 cgcgcaaagc ttccgcttgg cgagcggacg cggcacctgt acggtgttcc gtatcgtggc
      601 ccggggttgg tgccccgggg atcccctcag gaactgtggt atggcaatgg tcgccatgcc
      661 gtcgcagcgc ctgctcgatg aactcatgcg ctacatcgaa tttacgccgg aggatgcggc
      721 gcaaatgtat gcgatgggtc cggtgctgcg gccccacttc gcgcgggtcg tcgaccggtt
      781 ctacgaggcg atcgaccgca cgccggcggc ggcggcggtg ttcacgggtg gggcggagca
      841 gatcgagcgg caaaagacgc tgctgcacgg ctggctcgag ggcctggtcg gcggcgtgta
      901 cgacgacgcc tacctcgcgc ggcgggcccg gatcgggcgc acgcacgtgc ggatcgggct
      961 cgaccagcgc tacatgttct cgggcatgaa catcgtgcgg tccgagctgc acaacgcgct
     1021 ctacgacagc accgagtggc ccgccgacca gcagcgcagc gggcaccggg cgatcgaccg
     1081 catctgtgac atcgagctgg cgatcatgct cgagacctac cgcgaggcgt acatcgcccg
     1141 cgtcaaggag gccgagcggc tggcgacgat cgggcaggtc gccgcgtcga tcgggcacga
     1201 gctgcgcaac ccgctggcgg tgatgaagac gtcggtgcat atgctgacgc gccgcgtgcc
     1261 gagcgacgag cgcaccgtca agcacctgta ccgcctcgac gagcagatcg tgctctgcaa
     1321 tacgatcatc tcggacctgc tcgagctggc ccgcgaccgc ccggccgagc gcatcgccac
     1381 cgacatcgcc acggtcgtct cggaggcggc gctgtcggtc ccgcgcgtcg cgtcgatcgg
     1441 cctcggcctg cacatcgagc cgaagatccc gagcgtcgcg gtcgacccgg gccagtttcg
     1501 ccagctcgtc atcaacctcg tgctcaacgc gacccaggtg ctcgagggct ccgggcggat
     1561 cgaggtgcgc ctcggggccg acgagcaaaa cgtcgtgttg acggtcgagg acagcgggcc
     1621 gggcatcacc cccgatgtcc aggcgcgcct gttcgagccg ctgttcacga cccgctcgag
     1681 cggcaccggc ctcggcctcg cgctgtgccg gcgcgtcgtc gagaagcaca acgggtcgat
     1741 cgtcgccagc aaccgcgagg gcggaggggc ggccttcgtg gtccgcgtgc cgctcgcgtg
     1801 ggaggccgag tcgtgagcct cgaggccctc gtggtcgacg acaaccgcca gctggccgag
     1861 aacctcgccg agattctcgc ggaggagggc tgccgggtcc gcatggcctt tagcgcggag
     1921 gaggccctcg gcgcggccga ggcggtgtgc ccggacttcg tgctgaccga catccgcttg
     1981 cccggcatga acggcgtcga gctgatccgg cgcctcgtcg tccgggcgcc ggaggcgacg
     2041 tttctactga tgacggccta cacctccgat cagatcctcc gggacgcctc gcacctcggg
     2101 gtcgtgcgcg ccgtgctgcc caagccgctg gcgatcgaca ggctgctggc cctactgccg
     2161 cggcgaccgg ggagggcgat gtgagcgggc gcgtgctgat cgtcgaggac aacgtcgcgc
     2221 tcgccgagaa catcgccgag ctgttcgcgg acctcgaggt cgaggcggtg cgctgcgcga
     2281 ccggggccga ggcgctcgcg gccgccgacc cgaacatcga cctggcgatc gtcgacgtgc
     2341 gcctgcccga catcaccggc gtcgagctgc tgccgcggct gcgcccgcgc atgcccgacg
     2401 cggaggtgat cctgatgacc ggcgacgcct cgctggcgac ggcgatcgcg gcggtgcgcg
     2461 agggcgtgtt cgcgtacgtg caaaagccct tcgccccgca agacctgctc gcgctcggcc
     2521 tgcgcgcgca ggcccaggcc cagc
//
LOCUS       DASWNJ010000480         3610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_395598, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000480 DASWNJ010000000
VERSION     DASWNJ010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395598"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="VGB85_08200"
     CDS             <1..818
                     /locus_tag="VGB85_08200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HEY0134046.1"
                     /translation="GLSHRYPDRALLYVTHNCPVYCRHCTRQRKVGDATSAPDRASLA
                     AALDYVRRTPAIKDVLISGGDPLSLSDARLVEIVAELRAIDSVEIIRLCTRNPVTLPQ
                     RITPALLAALRPYHPIFVNTHFNHPRECTPEAAAALNLLADSGFAVGNQMVLLRGIND
                     DPAVVEQLNRWLLRQRCRPYYILQCDPVGGTAHLRTPVQAGVDILDALRGRVSGLGVP
                     QLVVDLPGGGGKVTLVPERLVRRIGTSMVFRNGQGREFTYVDGVPPGPAAGEG"
     gene            999..1835
                     /locus_tag="VGB85_08205"
     CDS             999..1835
                     /locus_tag="VGB85_08205"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEY0134047.1"
                     /translation="MRSSRLIRTVCLAAALPALVLTASPAQAADGSVNVLVIKEQGVG
                     SAAQAQPYVDKLVATTAKANGWAAAAGKYATNREGAEEYIHTADPHYGIMSLAAFLAL
                     RGKHKLEVLGKAEVTQAGGREYHIVSKNQADLAGCKGKTLASNHSDDVRFAEKVIGEG
                     AFTLGDFTLVATKRPVQTLKKVTSGEAECALIDDAQLAELKKIEGGAAVKSVWKSKQL
                     PPMVVVAFPSAPAAERTAFKAALGKVCGGEGKAACSEVGIKTLGAAGNAEYQAVITAY
                     GK"
     gene            2031..2807
                     /locus_tag="VGB85_08210"
     CDS             2031..2807
                     /locus_tag="VGB85_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134048.1"
                     /translation="MRRILLAATLVVMAVMTVAPVAVTGCKPHGGNLNNPAFADTKTS
                     FEIHERLEALIDQQKDSKQDREDAYALVKQRTGTTPEDLFARAAIAGRLAENRGLQAM
                     GLVTEAEDFARRCRAIEPDFREGAATRLLGSLYVLAPANLVKHGDSETGLELLEQEVQ
                     GHPERLVSRLRLAEAYIALNDPDPSREHLCVAKQGQDRLAAADKRLLARLLTDVGELK
                     CGSPETTSPPPDKTGDDKPGADKPGDDKPAAPAPPALSPM"
     gene            2825..>3610
                     /locus_tag="VGB85_08215"
     CDS             2825..>3610
                     /locus_tag="VGB85_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134049.1"
                     /translation="MHTRVSRPASRAGVRLAAIAWLGLCACNHQLPRLNARGEHAEVV
                     ERAAKAKHRPKRKAARAWADSLVALGRVEEARTVLLRDFRTTADLPSLIALADLEMHE
                     GLRGVAAAHYARAASLEVDPLRGREDVCALFRARAERFFHQGEAVAADLDLRRISVIC
                     PGPRGGDAARDPADRSLHLRVTAAAKAQAQAQRTLAGCKDGACKVAGAQVEQRRLTDA
                     LARARVTGHGALRQAARDLGVQLASGDVAELLAAELRGELGLDI"
BASE COUNT          550 a         1328 c         1260 g          472 t
ORIGIN      
        1 cagggctctc gcaccgttac cccgaccgcg ccctgctcta cgtgacgcac aactgcccgg
       61 tgtactgccg gcactgcaca cgccagcgca aggtcggcga cgccacttcg gcccccgacc
      121 gcgcgagcct ggcggcggcc ctcgattacg tgcgtcgcac gcccgcgatc aaggacgtgc
      181 tgatctccgg cggcgatccg ctgagcctca gcgacgcgcg cctggtcgag atcgtcgccg
      241 agctgcgcgc gatcgacagc gtggagatca tccgcctgtg cacgcgcaac ccggtgacgc
      301 tgccgcagcg gatcacgccg gcgctgctcg ccgcgctgcg cccgtaccat cccatcttcg
      361 tcaacacgca cttcaatcat ccgcgcgagt gcacgccgga ggccgccgcg gcgctcaact
      421 tgctcgcgga ctccggcttc gcggtcggca accagatggt cctgctgcgc ggcatcaacg
      481 acgaccccgc ggtcgtcgag cagctcaacc gatggttgct gcgccagcgc tgccggccgt
      541 actacatcct gcaatgcgac cccgtgggcg gcaccgcgca cctgcgcacg ccggtgcagg
      601 cgggggtcga catcctcgac gcgctgcgag gtcgcgtcag cggcctcggc gtcccgcagc
      661 tcgtggtcga cctgccgggc ggcggcggca aggtgaccct cgtgcccgag cgcctcgtcc
      721 gtcggatcgg caccagcatg gtgttcagga acgggcaggg ccgtgagttc acctacgtcg
      781 acggcgttcc gcccggcccc gccgcgggcg agggctgaaa aatcgccggc agggacacgc
      841 ggcgcctgcg tggtcggaag tcgcaaaccc gccaaacctg acgctgcatg gctgggaaag
      901 ccccgcaggg cgccgccggg aggctttctt cggggcgccg gtgacggcgt gcatacttcc
      961 cgacatcggg cgtccaaggc cgcccaggag ctacccacgt gcgatcttca cgcctcatcc
     1021 gtaccgtttg cctcgctgct gcgcttcctg ccctcgtcct gacggcgagc ccggcgcagg
     1081 cggccgatgg cagcgtcaac gtcctcgtca tcaaggagca gggggtcggc agcgcggccc
     1141 aggctcagcc ctacgtcgac aagctggttg cgacgaccgc caaggccaac ggctgggcgg
     1201 cggccgcggg caagtacgcg accaaccgcg agggcgccga ggagtacatc cacactgccg
     1261 atccgcacta cgggatcatg tcgctggccg ccttcctggc gctgcgtggc aagcacaagc
     1321 tcgaggtgct cggcaaggcc gaggtcacgc aggccggcgg ccgcgagtac cacatcgtca
     1381 gcaagaacca ggcggacctc gccggctgca agggcaagac cctcgccagc aaccacagcg
     1441 acgatgtccg tttcgccgag aaggtgatcg gcgagggtgc cttcacgctc ggcgacttca
     1501 cgctcgtggc gaccaagcgc ccggtgcaga cgctcaagaa ggtgacctcc ggcgaggccg
     1561 agtgcgccct catcgacgac gcgcagctcg ccgagctcaa gaagatcgaa ggcggcgccg
     1621 cggtgaagtc ggtgtggaag tcgaagcagc tcccgccgat ggtcgtcgtc gcgttcccct
     1681 cggccccggc cgcggagcgc accgccttca aggcggcgct cggcaaggtg tgcggcggcg
     1741 agggcaaggc cgcgtgcagc gaggtcggca tcaagactct cggcgccgcc ggcaacgccg
     1801 agtatcaggc cgtgatcacc gcgtacggca agtgatccgc gaccaaaact cgcgatggcg
     1861 agatcgcggt gacgagctga ccaggggcgg gcgcgcgggc gccggcccct cttgttttgc
     1921 ctcgaacgag cgtcgcgccg gtccgatctg ccccaccggc aaaaccccac gcaggggccg
     1981 tggcggcggc caggcgacac ttcggggaga gcatgatatg agtgggcggc atgcgtcgca
     2041 tcctccttgc agccaccctg gtcgtcatgg ctgtcatgac cgtcgccccc gtcgcggtca
     2101 ccggttgcaa gccccacggt gggaacctca acaacccggc cttcgccgac acgaagacct
     2161 cgttcgagat ccacgagcgc ctcgaggcgc tgatcgatca acaaaaggac agcaagcagg
     2221 accgcgagga cgcctacgcg ctggtcaagc agcggaccgg cacgaccccc gaggacctgt
     2281 tcgcccgcgc cgcgatcgcc ggtcggctcg ccgagaaccg cggcttgcag gcgatgggcc
     2341 tcgtcaccga ggccgaggac ttcgcccgcc gctgccgcgc gatcgagccc gacttccgtg
     2401 agggcgccgc gacgcggctg ctcggcagcc tgtacgtgct cgcgcccgcg aacctcgtca
     2461 agcacggcga ctcggagacc ggcctcgagc tgctcgagca ggaggtgcag ggccaccccg
     2521 agcgcctcgt cagtcggctg cggctcgccg aggcgtacat cgccctcaac gaccccgacc
     2581 cgagtcgcga gcacctgtgc gtcgcgaagc agggccagga ccggctcgcg gccgcggaca
     2641 agcgcctgct cgccaggctg ctgacggacg tcggcgagct caagtgcggc tcgcccgaga
     2701 cgacctcgcc gccgccggac aagaccgggg acgacaagcc cggggctgac aagcccggcg
     2761 acgacaagcc cgcggcgccg gccccgcccg cgctctcgcc gatgtgaacc ggaggaccct
     2821 cgctgtgcac acccgcgtct cccgccctgc gtcccgcgcc ggtgtccgcc tcgcggcgat
     2881 cgcgtggctg ggtctgtgcg cgtgcaatca tcagttgccg cggctcaacg cgcggggtga
     2941 gcacgccgag gtcgtcgagc gggcggcgaa ggccaagcat cgccccaagc gcaaggcggc
     3001 gcgcgcctgg gccgactcgc tggtcgcgct cggacgcgtc gaggaggcgc gcacggtgct
     3061 gctgcgagac tttcgcacga ccgccgacct gccctcgttg atcgcgctcg ccgacctcga
     3121 gatgcacgag gggctgcgcg gcgtcgcggc ggcccactac gcccgcgcgg ccagcctcga
     3181 ggtcgacccg ctgcgcggtc gagaggacgt gtgcgcgctg ttccgggccc gcgccgaacg
     3241 cttcttccat caaggcgagg cggtcgccgc cgacctcgac ctgcgcagga tctcggtgat
     3301 ctgccccggc ccgcggggcg gggacgcggc gcgtgatccc gccgatcgtt cgttgcatct
     3361 gcgggtcacg gcggccgcga aggcgcaggc gcaggcccag cgcacgctgg ccggctgcaa
     3421 ggacggcgcg tgcaaggtcg cgggcgcgca ggtcgagcag cgtcgcctca ccgacgcgct
     3481 ggcgagggcc cgcgtcaccg ggcacggcgc gctgcggcag gccgcgcgcg acctcggggt
     3541 gcaattggct tcgggcgacg tcgccgagct gctcgcggcg gagctgcgcg gagagctcgg
     3601 cctcgacatc
//
LOCUS       DASWNJ010000481         3190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_185527, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000481 DASWNJ010000000
VERSION     DASWNJ010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185527"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VGB85_08220"
     CDS             complement(<1..186)
                     /locus_tag="VGB85_08220"
                     /inference="COORDINATES: protein motif:HMM:NF026918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm53 family immunity protein"
                     /protein_id="HEY0134050.1"
                     /translation="MTDVMRRLQTWYASMCDGDWEHRFGVRITTLDNPGWHVRIDILE
                     TGLEIDEFVAVRVDRSDE"
     gene            complement(192..641)
                     /locus_tag="VGB85_08225"
     CDS             complement(192..641)
                     /locus_tag="VGB85_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134051.1"
                     /translation="MKIDRATGVVDVPELGVVLAPTTLRDAWLASAAGQGSIVGVQNE
                     PWWSFKARDFTVDGRPWKLSVGFHGQTPWRVELMALGAEFGTSWDDWSKTKEEARQVF
                     HDRWLTQVLGHGRSFAWGKVASVFDERGAYSSILIDYAVRDSLFARE"
     gene            complement(652..1290)
                     /locus_tag="VGB85_08230"
     CDS             complement(652..1290)
                     /locus_tag="VGB85_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134052.1"
                     /translation="MTRKLALCLALVFTTRTAQGAAPTAEAAPVSVDAALASGDLTAA
                     REQAEAARKASPGAASWAAEAEVCERLADLACAKAARTQQRELTAAGSPERAAVEAKL
                     AVLEDMSRGTVADEPRSKHRAELDRARSGRDMVAVVKPRPDIAPSKPVPPRERIVTKW
                     YFWVTLGAIAASAVAITVIGVKAAADEQSEKPEASAGRVRPGSLDHGFGIRF"
     gene            complement(1287..2861)
                     /locus_tag="VGB85_08235"
     CDS             complement(1287..2861)
                     /locus_tag="VGB85_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0134053.1"
                     /translation="MAGTAGQVSEIAGGATLAAPPGADADADLPLPAGTVIDGRYRLL
                     AELGSGGMGRVYEAEHMFIRRRMALKLLRTGAASDAAARMIQEATLAGQVPHPAVVRV
                     FDCAAIGDGKVYLAMELLRGESLEQALRRPGTAAEVVERVIEVARGLAAVHRVGVVHR
                     DIKPANIFLAQGEHGVHAKLLDFGIAKLLPGAVGAGADAVQTQAGAVLGTPYYVAPEQ
                     ARGGTIDGRADLYGLGVILYEAMTGVLPFVGGSFMEILAQHIRTPPLDPRQAAPGRPI
                     PDGLARLTMRLLAKDPAQRVSDGDALAEALEGLLRSEGEALARLVIGGAERGGTGADA
                     RTFVMADAGTVRLANAGGVAREGMSEGTGAMVGGRRWGIVGAAVIVVVIVVMIVVMGV
                     GWVRSGERAGEAVVAEPVVVPIVVSEPVVVSEPVPVAEPVPVSEPMPVSAVPGAGGSG
                     PAVVSPVRAVPEVEGAAQGPAGPFGPAVAGAGKPGKKRPRDGGTRRDARPPRTGGAAP
                     KTDPREPQLKPDVYDE"
     gene            2928..>3190
                     /locus_tag="VGB85_08240"
     CDS             2928..>3190
                     /locus_tag="VGB85_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134054.1"
                     /translation="MATGTKKIDELAALLEKTGGDAQRLEVVRRTQKFKRSWIELAEA
                     LTKVRATKGYARWGFEDFYDYCSRELTLKRATVDKLTISYNTI"
BASE COUNT          444 a         1342 c          963 g          441 t
ORIGIN      
        1 ctcgtcgctg cgatcgacgc ggaccgccac gaactcgtcg atctcgaggc ccgtctcgag
       61 gatatcgatc cgcacgtgcc agcccgggtt atcgagggtt gtgatgcgga ccccgaaccg
      121 atgctcccag tcgccgtcgc acatgctcgc gtaccacgtc tgcaagcgcc gcatgacatc
      181 cgtcatcgcc gtcactccct cgcgaacaac gagtcacgca cggcatagtc gatgaggata
      241 ctgctgtagg caccgcgctc gtcgaacacc gacgcgacct tgccccacgc gaagcttcgc
      301 ccgtgtccga gcacctgcgt cagccaccga tcgtgaaaca cctgccgcgc ctcctccttt
      361 gtcttcgacc agtcgtccca gctggtgccg aattcggccc cgagcgccat cagctcgacg
      421 cgccacggcg tctgcccatg aaaccccacg ctgagcttcc acgggcggcc atcgaccgtg
      481 aagtcgcgcg ccttgaacga ccaccagggc tcgttttgca cccccacgat gctgccctgg
      541 cccgccgccg aggccagcca cgcgtcacgc agggtcgtcg gcgccagcac cacgccgagc
      601 tccggcacat cgaccacccc ggtcgcgcga tcgatcttca tcgacgaccg ctcagaaccg
      661 gatcccgaag ccatgatcca gcgagccggg gcgtacccgg cccgcgctcg cctccggctt
      721 ctcgctctgc tcgtccgcgg ccgccttgac cccgatcacc gtgatcgcca ccgccgacgc
      781 cgcgatcgcg ccgagcgtga cccagaagta ccacttcgtc acgatgcgct cgcgcggcgg
      841 taccggcttg ctcggcgcga tgtccggcct gggcttgacc accgcgacca tgtcgcgtcc
      901 gctgcgcgcc ctgtcgagct cggcgcggtg cttcgagcgc ggctcgtcgg cgactgtccc
      961 gcgggacatg tcctcgagga cagccagctt cgcctcgacc gccgcccgct ccggcgagcc
     1021 cgccgcggtc agctcgcgct gctgggtccg cgccgccttc gcgcacgcga ggtccgccag
     1081 gcgctcgcac acctccgcct ccgccgccca gctcgccgcg cccgggctcg ccttgcgcgc
     1141 cgcctcggcc tgctcgcgcg cggccgtcag gtcgccgctg gcgagcgccg cgtcgacgct
     1201 caccggcgcc gcctcggccg tcggcgcggc cccctgtgct gtccgggtcg tgaacaccag
     1261 cgcgaggcag agggcgagct tacgcgtcat tcgtcgtaga catccggctt gagctgcggt
     1321 tcgcggggat cggtctttgg cgccgccccg ccggttcgcg gcggacgtgc gtctcgcctt
     1381 gtaccaccgt cgcgcggcct tttcttgccc ggcttgcccg cccccgccac ggccggcccg
     1441 aacgggcctg cgggcccctg cgccgcgccc tcgacctccg gcaccgcgcg gaccggcgac
     1501 acgaccgccg gccccgatcc ccccgccccc ggcaccgccg acacgggcat cggctccgat
     1561 acgggcaccg gctccgccac gggcaccggc tccgacacga ccaccggctc tgacacgacg
     1621 atcggcacga ccaccggctc cgccacgacc gcctcgcctg cccgctctcc cgaccgcacc
     1681 cacccgaccc ccatcacgac gatcatcacg acgatcacca cgacgatcac cgcagcgccg
     1741 acgatccccc accgccgacc gccgaccatc gcacctgtcc cttcggacat cccctcacgc
     1801 gccacacctc ccgcgttcgc caggcgcacc gtcccggcat ccgccatcac gaaggtccgc
     1861 gcatccgccc ccgtgccccc cctctccgcg cccccgatca ccagccgcgc gagcgcctcg
     1921 ccctcgctgc gcagcagccc ctcgagcgcc tccgcgagcg catcaccgtc gctgacgcgc
     1981 tgcgccggat ccttggccag cagccgcatc gtcagccgcg cgagcccgtc ggggatcggc
     2041 cgcccgggag cggcctgccg cgggtcgagc gggggcgtcc tgatgtgctg tgccaggatc
     2101 tccatgaagc tgccgccgac gaacggcagc acgcccgtca tcgcttcata aaggatcacg
     2161 ccgaggccgt agaggtcggc gcgcccgtcg atcgtcccgc cacgcgcctg ctcgggcgcc
     2221 acgtaatacg gcgtcccgag caccgcgccg gcctgcgtct gcaccgcgtc cgcgccggcc
     2281 cccacggcgc ccggcagcaa cttcgcgatc ccgaagtcga gcagcttcgc gtgcaccccg
     2341 tgctcgccct gcgccaaaaa aatgttggcc ggtttgatgt cacgatgcac caccccgacg
     2401 cggtgcaccg ccgccagccc gcgcgccacc tcgatcaccc tctcgaccac ctctgccgcc
     2461 gtccccggcc ggcgaagcgc ctgctcgagg ctctcgccgc gcagcagctc catcgccagg
     2521 tacaccttcc cgtcgccgat cgccgcgcag tcgaacaccc gcacgaccgc cggatgtggc
     2581 acctggcccg cgagcgtcgc ctcctggatc atccgcgcgg ccgcgtcgct cgcggccccg
     2641 gtgcgcagca gcttgagcgc cattcgccgg cggatgaaca tgtgctccgc ctcgtacacg
     2701 cggcccatgc cgccgctgcc gagctcggcg agcagccgat agcggccgtc gatcaccgtg
     2761 cccgccggca acggcagatc cgcgtcggcg tccgcgcccg ggggcgccgc gagcgtggcc
     2821 ccgcctgcga tctcgctgac ttggcctgcc gttcccgcca tcgcccgccc ctggtccgcc
     2881 gctcgccacg gtaccatccg gcgcgcggcg gtgctatatc gcggcccgtg gccacgggaa
     2941 ccaagaagat cgacgagctc gccgccctgc tggagaagac cggcggggac gcccagcgcc
     3001 tcgaggtcgt gcggcgcacg cagaagttca agcgctcgtg gatcgagctc gccgaggcgc
     3061 tgaccaaggt gcgcgcgacc aagggctacg cgcgctgggg cttcgaagat ttctacgact
     3121 actgcagccg cgagctgacc ctcaagcgcg ccaccgtcga caagctgacg atcagctaca
     3181 acacgatcca
//
LOCUS       DASWNJ010000482         2993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_503028, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000482 DASWNJ010000000
VERSION     DASWNJ010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503028"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2265
                     /locus_tag="VGB85_08245"
     CDS             <1..2265
                     /locus_tag="VGB85_08245"
                     /inference="COORDINATES: protein motif:HMM:NF018470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HEY0134055.1"
                     /translation="TIEPAREVTPTDLRPLFTSLRERSCEATRSPTVRRSAQRHSHAL
                     AAALALLACRLALTATTTASDTHARLQVLADLGATLDTGDPTPSLLALRAELDAWPVT
                     TPITLRGLFTPALRPTLLERYLQTAHERLLRPIWQALAADLGERGTHDQLRAYLLLTT
                     NDLSSEPGPLDPVQKDWLLTELPRLADSDTPGELTLLLTTLFEHATAGDLRFPRDHTL
                     VERARARLRALDDDDAVVRAALVAGNAACEPLSLKAITHAQQLSGASELPCSFTRSGW
                     RTVHAQLIRAAEHRDGWILGRPVREHADPARLARLARRYEALYIAAWTDFLAGLRIRR
                     PADLADASRLLAELTGDERPLAQIFAALEHHTRGLQRSSADHPSLTHLLQGLDRPEHS
                     AAIVRAFAPLLNFAASAPDRQAGLDRWHARLVELRDALEAARRDPADLPALHSQLTTA
                     LADTHALLQHTDLRRFRPLLSTLLLPPLTALQGELRDHGKLSLTRAYCSEIDAPLRRL
                     VTRYPFVRDAREEIGLDEFTAFFHPETGALRRFRDTQLTALITVHGFEVSANPGPRDE
                     DHPISPAVLAMFSRAAQLGALAFAGGELGLDLDLDLQCNADIGRVHLSLDGATHSYTC
                     GPDHHARMRWPGKAEPRGATLELLGRDGRRETVPGAGPWGLWRLLEKDGLVSPPQDRS
                     HPRLVFRLDLRASRLGTLDLAITPPRTHGATLLFGDGDPDAPLLAPLRAPELLDPPAT
                     LFVGLPTCAALETP"
     gene            2262..>2993
                     /locus_tag="VGB85_08250"
     CDS             2262..>2993
                     /locus_tag="VGB85_08250"
                     /inference="COORDINATES: protein motif:HMM:NF017730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HEY0134056.1"
                     /translation="MTRPSRPARARVDAQIHELDRKLATILGSILHHPKVQRLESLWR
                     GLHRLVTHTRPHENIAIDLLIVDKQSLHDDFLRADDLTHSGLHHHVYTRAYASHGGRP
                     YGLLCATFDFGPGADDLDLLRSCATVASLAHAPFVADAAPSLLGLRSLAEVPRLRDLA
                     AAQTGPRFTAWHAFRASPEARHVALCLPRFLLRAPHDSDTAPTRLPFREHIDRHEDLL
                     WGNASLLVAMRTAESFAEQRWCLGLV"
BASE COUNT          394 a         1435 c          785 g          379 t
ORIGIN      
        1 acgatcgagc ccgcccgcga ggtcaccccc accgacctcc gcccgctgtt caccagcctg
       61 cgcgagcgca gctgcgaggc cacccgcagc ccgaccgtcc gccgcagcgc ccagcgccac
      121 agccacgccc tcgcggccgc cctcgccctg ctcgcctgcc gcctcgcgct caccgccacc
      181 accaccgcga gcgacaccca cgcccgcctg caagtcctcg ccgacctcgg cgccaccctc
      241 gacaccggcg acccgacccc ctcgctgctc gccctgcgcg ccgagctcga cgcctggccc
      301 gtcaccaccc cgatcaccct gcgcggcctc ttcacccccg ccctgcgccc caccctgctc
      361 gagcgctacc tacaaaccgc ccacgagcgc ctgctccgac cgatctggca ggcactcgcc
      421 gccgacctcg gcgagcgtgg cacccacgac cagctccgcg cctacctcct gctcacaacg
      481 aatgacctgt cctcggagcc aggtcccctc gaccccgtac aaaaagactg gctgctcacc
      541 gagctgccgc gcctcgccga cagcgacacc cccggcgagc tcaccctcct gctgacgacc
      601 ctgttcgaac acgccaccgc cggggacctg cgcttccctc gcgaccacac cctcgtcgag
      661 cgagcccgcg cccgcctgcg cgcgcttgac gacgacgacg ccgtcgtacg cgccgccctc
      721 gtcgccggca acgccgcctg cgagccgctc tccttgaaag cgatcaccca cgcgcagcag
      781 ctgagcggcg ccagcgagct cccgtgcagc ttcacgcgct ctggctggcg caccgtgcat
      841 gcgcaactga tccgcgccgc cgagcaccgc gacggatgga tcctcggccg cccggtgcgt
      901 gagcacgccg acccggcccg cctcgcccgc ctcgcccgcc gctacgaggc cctctacatc
      961 gcagcctgga ccgacttcct cgccggcctg cgtatccgtc gcccggcaga cctcgccgac
     1021 gccagccgcc tgctcgccga gctcaccggc gacgagcgcc cgctcgccca gatcttcgcc
     1081 gccctcgagc atcacacccg cggcctccag aggtccagcg ccgaccatcc ctcgctgacc
     1141 cacctcctgc aaggcctcga tcgccccgag cacagcgccg cgatcgtccg cgccttcgcc
     1201 ccgctgctca acttcgccgc ttccgccccc gaccgccagg ccggcctcga ccgctggcac
     1261 gcccgcctcg tcgagctgcg cgacgccctc gaggccgcgc gccgcgaccc cgccgatttg
     1321 cccgcgctgc acagccagct gaccaccgcg ctcgccgaca cccacgccct cctgcaacac
     1381 accgacctgc gccgctttcg tccgctgctc tccaccctcc tgctgccccc cctgaccgca
     1441 cttcaaggcg agctgcgcga ccacggcaag ctgtccctga cgcgggccta ttgcagcgag
     1501 atcgacgccc ccctgcgccg cctcgtcacc cgctacccct tcgtccgcga cgcccgcgag
     1561 gagatcggcc tcgacgagtt caccgccttc ttccaccccg agaccggcgc cctgcgtcgc
     1621 ttccgcgaca cgcagctgac cgccctcatc accgtccacg gcttcgaggt ctccgccaac
     1681 cccggccccc gcgacgagga ccacccgatc tcccccgccg tgctcgctat gttcagccgc
     1741 gccgcgcaac tcggcgccct cgccttcgcc ggcggcgagc tcggcctcga cctcgacctc
     1801 gacctccagt gcaacgccga catcggccgc gtccacctca gcctcgacgg cgccacccac
     1861 agctacacct gcggccccga ccaccacgcc cgcatgcgct ggcccggcaa ggccgagccc
     1921 cgcggcgcca ccctcgagct cctcggtcgc gacggccgcc gcgaaaccgt gcccggcgcc
     1981 ggcccctggg gcctgtggcg cctgctcgag aaggacggcc tcgtctcccc gccacaggac
     2041 cgcagccacc cccgcctcgt gttccgcctc gacctccgcg ccagccgcct cggcaccctc
     2101 gacctcgcca tcacgcctcc ccggacccac ggcgccaccc tgctgttcgg cgacggcgac
     2161 cccgacgccc cgctgctcgc ccccctgcgc gccccggagc tgctcgaccc gcccgcgacc
     2221 ctgttcgtcg gcctgcccac ctgcgccgcg ctcgagacgc catgacccgc ccctcacgac
     2281 ccgcccgcgc ccgcgtcgac gcccagatcc acgagctcga ccgcaagctc gccacgatcc
     2341 tcggctccat cctgcatcac ccgaaggtcc agcgcctcga gtccctgtgg cgcggcctgc
     2401 atcgcctcgt cacacacacg cgcccgcacg agaacatcgc catcgacctg ttgatcgtcg
     2461 acaaacagtc tctccacgac gacttcctgc gcgccgacga cctcacccac agcggcctcc
     2521 accaccacgt ctacacacgc gcctacgcga gccacggcgg ccgcccctac ggcctgctgt
     2581 gcgccacctt cgacttcggc cccggcgccg acgacctcga cctcctgcgc agctgcgcca
     2641 ccgtcgcgag cctcgcccac gcccccttcg tcgccgacgc cgccccgtcc ctgctcggcc
     2701 tgcggtccct cgccgaggtc ccccgcctgc gtgacctcgc cgccgcccag accggccccc
     2761 gcttcaccgc ctggcacgcc ttccgggcct ccccggaggc ccgccacgtc gccctctgcc
     2821 tcccgcgctt cctgttgcgc gccccccatg acagcgacac cgccccgacc cgactgccct
     2881 ttcgcgagca catcgaccgc cacgaggacc tgctgtgggg caacgccagc ctgctcgtcg
     2941 ccatgcgcac cgccgagtcc ttcgccgagc agcgctggtg cctcggcctc gtc
//
LOCUS       DASWNJ010000483         3913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_114491, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000483 DASWNJ010000000
VERSION     DASWNJ010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114491"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="VGB85_08255"
     CDS             <1..978
                     /locus_tag="VGB85_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971703.1"
                     /note="catalyzes the conversion of salicylyl-CoA to
                     gentisyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HEY0134057.1"
                     /translation="WHLVHLGGLALGGAGLVMTEMTDVSPTGRITPGCAGIYHDAHTQ
                     AWARIVEFVHTRSASKIGVQLGHAGRKGAVTHPWTRATDVPLPDDQAWELLAPSAIAW
                     APGYATPRPMTTQDMDQVVQDFVMATHRAIAAGFDLIELHMAHGYLLSSFISPLTNTR
                     DDEFGGDIAGRMRFPLRVFNEVRAVWPADRPISVRISASDWAEGGLTGDDAVAVARVL
                     QAAGGDLLDVSAGQTTPYGKPVYGRMFQTHFSDQIRNTLGIATIAVGNIQDWDQVNTI
                     VASGRADLCALARPHLVDPHFTLRAALEQNYSGPGAVWPPQYLAAAPRG"
     gene            complement(985..2532)
                     /locus_tag="VGB85_08260"
     CDS             complement(985..2532)
                     /locus_tag="VGB85_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0134058.1"
                     /translation="MKLEWSWTANEVSVLPLVADIAGDDRPEVIVNASRTDGIAREIG
                     EIVVLDGVTGTELWRTKETPQGDSHGSFGLGTPAVGDLDGDLRPDIVYPGRVSGFPEK
                     KRGPVHAVDGAGKHLWTGHTADNTPVLVDWQHGAAALVNLDDDSAAEVAIGGVLFDND
                     GLLVWNQGGNGGTLGTPTANNNPPDLLYSGSLATFADLTGDGRPELLTGREAWTITWT
                     PGSPPAVSMSMLWKNSDGEGNDGWPALGDLDQDGAPEVVLVAWPDIKVLDGKTGKLWC
                     GVDPTGTMCADNDALRTPPIEIAGGNLGGPATIADFDGDGRPEVGIAAGKAYAVYDFA
                     RPGEELIFPDNAPSPAPGAMFLRWSTPTQDNSSGSNGSSVFDFQGDGVPEVLYHDECK
                     LRIYDGSTGQTRHEELSSSGTVHEYPPVVDVDGDGHAEFLAVSNLSEPVVNAQCQSAD
                     PAFVPRQGVFAYGADGEPWAATRKLWTQHTYHVTNAHADGNVPMTEQPNWTVPGLNNF
                     RQNVAGD"
     gene            complement(2930..>3913)
                     /locus_tag="VGB85_08265"
     CDS             complement(2930..>3913)
                     /locus_tag="VGB85_08265"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0134059.1"
                     /translation="KLLDFGIARVQGGGDLTAVGAQLGSPNYMAPEQVLAQPLDGRTD
                     IYSLGVLLFELLTTELPFQGSPVEVAMQHCNATPPRPSQLAPEYEIPLPLEAVVLTAM
                     ARNPDQRYPTMEALEAALTALLPTTAPRQGPPLPATPETTPGAPTLVDAVSDDTETPR
                     SVASPRRQGRRIVVLGVLAAALGGLAVLPFLPAASPPAPAGGPTIGPAIGPAIMHVTE
                     AAAAPVEPVVTPTPGPIPPEPAIAKTPTRGPREAKTMDPRRELERKAQACRRKHDAAA
                     PPITIDYAVRSDGTVSRAIAAKTGPLATCLIEATKSTRFPPRLALGQSITL"
BASE COUNT          537 a         1280 c         1483 g          613 t
ORIGIN      
        1 tggcacctgg tgcacctcgg cgggctggcg ctcggcgggg cggggctggt gatgacggag
       61 atgaccgacg tgtcgccgac cgggcgcatc acgccgggct gcgcgggaat ttatcatgac
      121 gcgcacacgc aggcgtgggc gagaattgtc gagttcgtgc atacgcgcag cgccagcaag
      181 atcggcgtgc agctcgggca cgccgggcgc aagggggctg tcacgcaccc gtggacaaga
      241 gcgacggacg tgccgctgcc ggacgatcag gcctgggaat tgctcgcgcc gtcggcgatc
      301 gcgtgggcgc cgggatatgc gaccccgcgg ccgatgacga cgcaggacat ggaccaggtc
      361 gtgcaggatt ttgtcatggc gacgcaccgg gcgatcgcgg ccggcttcga tttgattgaa
      421 ctgcatatgg cgcacggata tctgctgtcg agcttcatct cgccgctgac gaacacgcgg
      481 gacgatgaat tcggcggtga catcgcgggg cgcatgcgct tcccgctgcg agtttttaat
      541 gaggtgcgcg cggtgtggcc ggcggaccgg ccgatctcgg tgcggatctc ggcgagcgac
      601 tgggccgagg gcggcctgac cggcgacgac gccgtcgcgg tcgcgcgcgt gctgcaggcg
      661 gccggcggcg atctgctcga cgtttcggcc gggcagacga cgccgtacgg caagccggtg
      721 tatggccgca tgttccagac ccacttttcc gaccagatcc gcaacacgct cggcattgcg
      781 acgatcgcgg tcggtaacat ccaggactgg gatcaggtca atacgatcgt cgcctcgggg
      841 cgcgccgacc tgtgcgcgct ggcccggccg cacctcgtcg acccacactt cacgctgcgc
      901 gcggcgctcg agcagaacta cagcggaccc ggcgcggtgt ggcccccgca gtacctcgcc
      961 gccgcgccgc gggggtgagg cccgtcagtc gcccgccacg ttctggcgga agttgttgag
     1021 gccgggcacg gtccagttgg gctgctcggt catcggcacg ttgccgtcgg cgtgcgcgtt
     1081 ggtgacatga taggtgtgct gcgtccacag cttgcgggtc gcggcccagg gctcgccgtc
     1141 ggcgccgtag gcgaacacgc cctggcgcgg tacgaacgcg gggtcggccg actggcattg
     1201 cgcattgacg acgggctcgc tgaggttgga gacggcgagg aactccgcgt gaccgtcacc
     1261 gtcgacgtcg acgacgggcg gatactcgtg cacggtgccg ctcgagctga gctcctcgtg
     1321 gcgcgtctgg ccggtgctgc cgtcgtatat gcgcagcttg cattcgtcgt gatagagcac
     1381 ctccgggacg ccgtcaccct ggaagtcgaa caccgaggat ccgttcgaac cggaggagtt
     1441 gtcctgcgtg ggcgtggacc agcgcaggaa catcgcgccg ggggccggtg aaggggcgtt
     1501 gtcggggaag atcagctcct cgcccggacg cgcgaagtcg tagacggcgt aggccttgcc
     1561 cgcggcgatg ccgacctccg ggcgcccgtc gccgtcgaag tcggcgatcg tcgccggacc
     1621 accgaggttg ccgcccgcga tctcgatcgg cggcgtgcgc agggcgtcgt tatctgcgca
     1681 catggtgccc gtcgggtcga cgccgcacca cagcttgccg gtcttgccgt cgagcacctt
     1741 gatgtccggc caggcgacga gcacgacctc cggggcgccg tcctgatcga ggtcgccgag
     1801 cgcaggccag ccgtcgttgc cctcgccgtc gctgttcttc cacagcatcg acatcgatac
     1861 cgcgggcggg ctgccggggg tccaggtgat cgtccaggcc tcgcgtccgg tgagcagctc
     1921 gggcctgccg tcgccggtca ggtcggcgaa ggtcgccagg ctgccagaat agaggagatc
     1981 tggcgggttg ttgttcgcgg tcggcgtgcc gagcgtgccg ccattaccac cctggttcca
     2041 caccagcagg ccgtcgttgt cgaacaatac cccgccgatc gcgacctcgg ccgcgctgtc
     2101 gtcgtcgagg ttgaccagcg ccgcggcgcc gtgctgccag tcgaccaaaa cgggggtgtt
     2161 atcggcggtg tgcccggtcc acaggtgctt gccggcgccg tcgaccgcgt gcaccggacc
     2221 gcgcttcttc tcggggaagc cggagacgcg gccgggatag acgatgtcgg ggcgcaggtc
     2281 gccgtcgagg tcgccgacgg ccggggtgcc gaggccgaag gagccgtggc tgtcgccctg
     2341 cggggtctcc ttggtgcgcc acagctccgt gccggtgacg ccgtcgagca cgacgatctc
     2401 accgatttcg cgcgcgatcc cgtcggtccg cgacgcgttg acgatcacct cggggcggtc
     2461 gtcacccgcg atgtcggcga cgagcggcaa gaccgagacc tcgttggccg tccacgacca
     2521 ctcgagcttc acgccgagct gcggggctcc gccggtcggc tcggtatctc cggagtcccc
     2581 ggtcccgctc gcgccctctg tcgcacccgc gatgtcatcc ccgcaaccca ccagcgcgac
     2641 gaggcatatc accaaccgat tatttttcat gaccctgagc gttacgcacg cgatgccacg
     2701 cggcaagctc cgaccgccac aaagtggtcc gcgtcaggtc ccgcagcttc gccgcggcag
     2761 tgacacattg tcacgagggc tggcgcatcg atgaaggccg ccagtcactg cgcggtgcgt
     2821 gcaggtcgct cgccggcccc ggtgacgagc gtcacctctc agcgcacgcc gacgcgctcg
     2881 gcgacgcgca gccaggtgtc ggcgaacgcc aggaggcgca ggcgcgagat cacagcgtga
     2941 tcgattgacc gagggcgagg cgcggcggga agcgggtcga cttcgtggct tcgatcaggc
     3001 aggtggcgag cgggccggtc ttcgcggcga tcgctcgcga gacggtgccg tcgctgcgca
     3061 cggcgtagtc gatcgtgatc ggtggggcag cggcgtcgtg cttgcggcgg caggcctgcg
     3121 ccttgcgctc gagctcgcgg cgcgggtcca tggtcttcgc ctcgcggggg ccgcgggtcg
     3181 gcgtcttggc gatcgcaggt tcgggtggta tcggcccggg tgtcggcgtg acgacgggct
     3241 cgaccggcgc ggcggcggct tcggtcacgt gcataatcgc ggggccgatc gcggggccga
     3301 tcgtcgggcc gccggcgggc gcgggcgggc tggcggccgg gaggaagggc agcacggcga
     3361 ggccgccgag cgcggccgcg aggacgccga ggacgacgat acgtcgaccc tgcctgcgcg
     3421 gtgacgcgac gctgcgcggg gtctcggtgt cgtcggagac cgcgtcgacg agcgtcggtg
     3481 cgccgggcgt ggtctcgggc gtcgcgggca gcggtgggcc ctggcgcggg gcggtcgtcg
     3541 ggagcagggc ggtcagcgcg gcctcgaggg cctccatcgt cgggtagcgc tggtcggggt
     3601 tcctggccat cgcggtgagc acgacggcct cgagcgggag cgggatctcg tactcggggg
     3661 cgagctggct ggggcggggc ggggtcgcgt tgcaatgttg catcgccacc tcgaccgggc
     3721 tgccctgaaa cggcagctcg gtcgtcaaca gctcgaacag caggacaccg agcgaataga
     3781 tgtcggtgcg gccgtcgagc ggctgggcga gcacctgttc gggtgccatg taattgggcg
     3841 agccgagctg ggcgccgacg gcggtcaggt cgccgccgcc ctggacgcgg gcgatgccga
     3901 agtcgagcag ctt
//
LOCUS       DASWNJ010000484         2694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_608900, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000484 DASWNJ010000000
VERSION     DASWNJ010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608900"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2379)
                     /locus_tag="VGB85_08270"
     CDS             complement(<1..2379)
                     /locus_tag="VGB85_08270"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0134060.1"
                     /translation="MIEISGIELQAEIGQGARSVVYRGRRGDRLVAVKLLRRVSGSDG
                     RAQALQVRREAAALASLRHPCFVEILEVGETREQPYLVMEYVAGRTVAELLARGKMPE
                     SQAVRIGHTVAVALTEVHRRGMVHRDIKPQNILLADAGGLKIIDFGFATHAHTVEDPA
                     QIAGTFLYSAPEQTGMLRRPLDGRADLYALGAVLFECVAGRPPFLAADVSELLRMHAV
                     ARPPDLRELVPGCSRGLAAIVARLLAKDPDDRYQTGAGLASDLARVDRLGDPPVLGSF
                     DDAREVSDDAPLVGRAAELAQLEALWTRALRGHGGLATIEGEPGSGKSRLIRALFSSV
                     PGMAATPGPLTRNNSEPRRTTPAPNSLFRPATHVPGGPTPPAGGATPLPSRAAPLVLV
                     GSCEAGDAAPFAALRQGFGDLVRHLRRLSAGARAEASFRLDEAAAERRGLLLQFCPEL
                     KDMFSRTDPAPPDAPGVEANDQLHAAVVEYLLALAHAHGGLLLVVDDVQWIDESSLLV
                     LRALAARVAAAPLLCLCSLRTGAGERFLREIGPTLSARIPVPPLDEASVGALVSELLG
                     GGDPGPEIVRQVVTRSDGSPLAVGEYVRAMQASGLLCPHWGGWRLDAAGLESLRLPGD
                     VLRLIVDRVVGLDAGTRAVLAVAAIIGGRFTLDLLATVAERPREAVDAAITEAVSARL
                     IDRGDAGRYIFVHDRVREALLVGLDDGTRRGLHQRIALALDRGEATPLADIYARARHY
                     EAGDRSDPARLVAACTAAGLAALADYASEDAYGALHQAHAVAEQHGLEIEPRL"
BASE COUNT          351 a          948 c         1072 g          323 t
ORIGIN      
        1 cagccgcggc tcgatctcca ggccgtgctg ctcggcgacc gcgtgggcct gatgcagcgc
       61 gccgtaggcg tcctcgctcg catagtcggc gagcgcggcg aggcccgcgg ccgtgcacgc
      121 ggcgaccaag cgcgccgggt cgctgcggtc gccggcctcg tagtgacgcg cgcgcgcgta
      181 gatgtcggcg agcggggtgg cttcgccgcg gtcgagcgcg agggcgatgc gctggtgcag
      241 gcctctgcga gtcccgtcgt cgaggccgac gagcaaggcc tcgcgcacgc ggtcgtgcac
      301 gaagatgtag cgcccggcgt cgccgcgatc tatcaagcgc gccgagacgg cctcggtgat
      361 cgcggcgtcg acggcctcgc gcgggcgctc ggcgacggtg gcgagcaggt cgagggtgaa
      421 gcgaccgccg atgatcgccg cgaccgcgag cacggcgcgg gtgccggcgt cgaggccgac
      481 gacgcggtcg acgatcaggc gcagcacgtc gccgggcagg cgcaggctct cgaggccggc
      541 ggcgtcgagg cgccagccgc cccagtgcgg gcacagcagc ccggaggcct gcatcgcgcg
      601 cacgtactcg ccgacggcga gcgggctgcc gtcgctgcgc gtgacgacct gccggacgat
      661 ctccgggccc gggtcgccgc cgccgagcag ctcggagacg agggcgccga cggaggcctc
      721 gtcgagcgga ggcacgggga tccgcgcgct cagggtcggg ccgatctctc gcaggaagcg
      781 ctcgccggcg ccggtgcgca gcgaacacag gcacagcagc ggggccgcgg cgacccgcgc
      841 ggcgagggcc cgcaggacca gcaggctgct ctcgtcgatc cactgcacgt cgtcgacgac
      901 gagcagcagg ccgccgtgcg cgtgcgcgag ggcgagcaga tactcgacga ccgccgcgtg
      961 cagctggtca ttcgcctcga cgcccggggc gtccggcggc gcggggtcgg tccgcgagaa
     1021 catgtccttg agctcgggac agaactgcaa caacaggccc cggcgctcgg ccgcggcctc
     1081 gtcgaggcga aagctggcct cggcgcgcgc gcccgcggag agccgccgca ggtgacggac
     1141 gaggtcgccg aagccctggc gcagcgcggc gaacggggcg gcgtcgccgg cctcgcagct
     1201 gccgacgagc accagcggcg cggcgcgaga cggcagcggg gtcgcgccgc cggccggggg
     1261 ggtcggtccg ccgggcacgt gcgtcgccgg tcggaacagg ctgttggggg ccggggtggt
     1321 gcgtcgcggc tcgctgttgt tgcgcgtcag cgggccgggg gtcgcggcca tgccgggcac
     1381 ggaggagaac agggcgcgga tgaggcggct cttgccgctg ccgggctcgc cttcgatcgt
     1441 ggcgaggccg ccgtggccgc gcagggcccg ggtccacagc gcctcgagct gcgcgagctc
     1501 ggcggcgcga ccgacgagcg gggcgtcgtc gctgacctcg cgcgcgtcgt cgaagctgcc
     1561 gagcaccggc gggtcgccga ggcggtcgac gcgggcgagg tcggaggcca gcccggcccc
     1621 ggtctggtag cggtcgtcgg ggtccttggc gagcaggcgc gcgacgatcg ccgccagccc
     1681 gcgcgagcag ccgggcacca gctcgcgcag gtcgggcggg cgggcgaccg cgtgcatgcg
     1741 caacagctcg ctgacgtcgg cggcgaggaa gggcggccgg ccggcgacgc actcgaacag
     1801 cacggcaccg agcgcgtaga ggtcggcgcg gccgtcgagc gggcgccgca acatcccggt
     1861 ctgctccggc gcgctgtaca aaaaagtgcc ggcgatctgg gccgggtcct cgaccgtgtg
     1921 cgcgtgggtg gcgaagccga agtcgatgat cttgaggccg ccggcgtcgg cgagcaggat
     1981 gttctgcggc ttgatgtcgc gatgcaccat gccgcggcgg tggacctccg tcagcgcgac
     2041 cgcgaccgtg tggccgatgc ggaccgcctg cgactcgggc attttgccgc gcgcgagcag
     2101 ctcggcgacg gtgcgtcctg ccacgtactc catcacgagg tagggctgct cgcgcgtctc
     2161 gccgacctcg aggatctcga cgaagcaggg gtgacgcagg ctcgccagcg cggcggcctc
     2221 gcgtcgcact tgcagcgcct gggcccggcc gtcgctgccg gacacgcgcc gcagcagctt
     2281 gaccgcgacg aggcgatcgc cacgtcgccc gcgatagacg acgctacggg cgccctgccc
     2341 gatctccgct tgcagctcga tgcctgaaat ctcgatcacg ttgcggcgcc gtcgacatgt
     2401 tcacccatgc ccgcgccggg cgaaagccgc ccgcagaatg acgttcactc gcccgtctgc
     2461 gcctgtgcgc ggggtcgcac ggtcggccgg ggttgccaga cgggccgggc cgggcgccgg
     2521 ggcctcgcgg gcctcgcggg cgcacggcgt ccgcggccgg cagcggcgcg ctctgggcgg
     2581 tccggggcgc gcctggacag cacatcacgc tttcagataa aagccacagg tcatggctga
     2641 tacacatctc gcacaaatgt tcttcgaccg cgcccggtct ggtggctcgg aggc
//
LOCUS       DASWNJ010000485         5052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_80160, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000485 DASWNJ010000000
VERSION     DASWNJ010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80160"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="VGB85_08275"
     CDS             complement(<1..1304)
                     /locus_tag="VGB85_08275"
                     /inference="COORDINATES: protein motif:HMM:NF027993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0134061.1"
                     /translation="MLHLLRPVPALLIALAPLAGCDSPDTDAGARAVTEYREVWGTQD
                     NPSLLDPGFNYTFAQLPTSGAAKKTPWTGSYWPTYQDSINMRWAGAASQSAARKYELA
                     FGKTGVEDAVSAASGIDSLAGAACTSDAQCDAGKGSVCAKRAGSSEGTCSETWFGLCH
                     AWAPAAILEDEPKLPVTVNGVEFKVNDLKALMSLAYTDALDVKFMSLRCDDSAKTGDL
                     TGKAACKDTNAGSFHVVVANLLGIQGKAFVEDRTYDYEVWNQPVRSFKVTKNVALTPT
                     QANQLLGAGKTLSAADRAGSVAASAWTQVYNLAVKPGQTLRVRMTGTGDADLYLRWNA
                     QPTATTHSCRPYLEGSDELCELVVPADAKTAYIAVNGYAPTSQYKITTTLIDLAPATY
                     AYNPAAVSLRQIQTELRWIGESASTTDGNLSAVIDQYTQKDV"
     gene            complement(1480..1692)
                     /locus_tag="VGB85_08280"
     CDS             complement(1480..1692)
                     /locus_tag="VGB85_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134062.1"
                     /translation="MSEPTEADHIERITDRLRRDHPDPKMRQQLYDEGKLPEYVRTLM
                     QEADDEALLERVTAAVRAAEDGKSAE"
     gene            complement(1714..2064)
                     /locus_tag="VGB85_08285"
     CDS             complement(1714..2064)
                     /locus_tag="VGB85_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134063.1"
                     /translation="MRTRLLLIATLATLTAACGYRVDNCDQPSKGFSLDEELDERAVE
                     LLVRDSRVIDHTQLECDMVCELIYLDQNPKGGATEVEVCDLVLDGDFTGDPDAVVGSI
                     YCEGRGIPQFCVDA"
     gene            complement(2169..3038)
                     /locus_tag="VGB85_08290"
     CDS             complement(2169..3038)
                     /locus_tag="VGB85_08290"
                     /inference="COORDINATES: protein motif:HMM:NF016858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134064.1"
                     /translation="MSRPPGDLWLDVLRPPPGFFLSTTALSRVDEAVLRLRTREVKKP
                     ETLRERTLAPEPGGLFCDQIFGEGPLQRGPFADDEPVTEARATRFGRIELPAPVVHPL
                     ALAHAPDEVAARAGISRDELDRLVAYTDPAQWPRLGELLARTADGAALLLRVLRVLPP
                     YLRPIKRLDDYRWVTSDINDLYRRVINRVNRLKRLLELAAPVVIVAEEHKFLGEAIHM
                     LFANEDRPAPATDVAGRPYVSLRTLAVGNRLFEALSELDRRGPPGDAETRPLPRALHH
                     PIAALHAMGLELG"
     gene            3208..3516
                     /locus_tag="VGB85_08295"
     CDS             3208..3516
                     /locus_tag="VGB85_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134065.1"
                     /translation="MTLPFVLAVALLGACGTKEAPANDASRGKADAATAGGVGIPPVT
                     GTPRGPQGVETPPAPPNGGNAPPGDRNSGVGTIVGTDEAKAADAKSADGSTKQERHAQ
                     "
     gene            complement(3537..3956)
                     /locus_tag="VGB85_08300"
     CDS             complement(3537..3956)
                     /locus_tag="VGB85_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134066.1"
                     /translation="MTSSWTTLRYREAYGAPGDDRAGHVEMTLWCDRRVRVAHMLARP
                     AVFEGTLAEWVWPELEMQLRAAGFPTLSRPATAQGRGALLSTATENGALQMMWIWPEL
                     RKNVRLDRAVRVLEAIAHAASAGSLKLYRAIEREVLA"
     gene            4149..>5052
                     /locus_tag="VGB85_08305"
     CDS             4149..>5052
                     /locus_tag="VGB85_08305"
                     /inference="COORDINATES: protein motif:HMM:NF025719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY0134067.1"
                     /translation="MEAVHEVLAERSEEVMLRWKATVRGTLAPESMPVVELVDHLPMF
                     LREVIVALREDRGLTATYQPQTEASRTAAEHGEQRLRLGFSLDSVVREYGVLRDAIVA
                     TVRDAGIDVSFRELQIVFDAIIDGIAHAVSEYTRQRDAELFRQHNEHVAFMAHELRNP
                     LGSARMAVALLKRGGHIPADARGAAALDRGLQRMQEGIDHSLTVARTASGIAVRREKT
                     RLRTLLEDVEIGALGEAQDKGIDLRLHLEQDAEIEVDVRLISSALGNLVRNAVKYSCA
                     SGSVEIRGGISVIGVRIEVEDSCGG"
BASE COUNT          727 a         1656 c         1882 g          787 t
ORIGIN      
        1 tagacgtcct tttgcgtgta ctgatcgatg acggccgaga ggttgccatc ggtggtgctc
       61 gccgactcgc cgatccaccg cagctcggtc tggatctggc gcaggctgac ggccgcgggg
      121 ttgtaggcgt aggtcgcggg cgcgaggtcg atcagggtgg tcgtgatctt gtactgcgag
      181 gtcggggcgt agccgttgac ggcgatgtac gcggtcttgg cgtcggccgg cacgaccagc
      241 tcgcacagct cgtcgctgcc ctcgaggtac gggcggcagc tgtgggtggt cgcggtcggc
      301 tgtgcgttcc agcgcaggta caggtcggcg tcgccggtcc cggtcatgcg cacgcgcagg
      361 gtctggcccg gcttgaccgc gaggttgtag acctgcgtcc acgcgctcgc ggcgacgctg
      421 ccggcccggt cggcggcgct gagggtcttg ccggcgccga gcagctggtt ggcctgggtg
      481 ggcgtcagcg cgacattctt cgtgaccttg aagctgcgca ccggctggtt ccacacctcg
      541 taatcatagg tgcggtcctc gacgaaggcc ttgccctgga tcccgagcag gttggcgacg
      601 acgacgtgga agctgccggc gttggtgtcc ttgcaggcgg ccttgccggt caggtccccg
      661 gtcttggccg agtcgtcgca gcgcagcgac atgaacttga cgtcgagcgc gtcggtgtag
      721 gcgaggctca tcagcgcctt gaggtcgttg accttgaact cgacgccgtt gacagtgacc
      781 ggcagcttcg gctcgtcctc gaggatcgcc gcgggcgccc acgcgtggca caggccaaac
      841 cacgtctcgc tgcaggtccc ctcgctgctg ccggcccgct tcgcacacac cgaacccttg
      901 ccggcgtcac actgcgcgtc gctggtacac gcggcgcccg cgaggctgtc gatgccgctc
      961 gccgccgaca cggcgtcctc gaccccggtc ttgccgaacg cgagctcgta cttgcgggcc
     1021 gccgactggc tggccgcgcc ggcccagcgc atgttgatgc tgtcctgata ggtcggccag
     1081 taactgccgg tccacggcgt cttcttggcg gcgccgctgg tcggcagctg ggcgaaggta
     1141 tagttgaagc ccgggtcgag caggctcggg ttgtcctgcg tgccccacac ctcgcgatac
     1201 tcggtcacgg cgcgcgcgcc agcatcggta tcgggcgagt cacagccggc gagcggcgcg
     1261 agagcgatca acagggcggg gacggggcgg agcaggtgca gcacggccgg cgcctgagca
     1321 agcgccgtgc caactgtgtt gtagggtgcg agcgaggcgc cgatgtgggt ggatctggtg
     1381 gcgtgcgcgg aaaggtgtgg ctactcgagt cgccaggagc catgcaggtg gctagaaacg
     1441 ttgtttttgg tgctccgggc ggaccgcgcc gcaggcaggt tactcggcgg actttccgtc
     1501 ctcggcggcg cggacggcgg cggtgacccg ctcgagcagc gcctcgtcgt cggcctcctg
     1561 catcagcgtg cgcacatatt cggggagctt gccctcgtcg tagagctgct ggcgcatctt
     1621 cggatccggg tgatcacggc gcaggcggtc ggtgatccgc tcgatatggt cggcttcggt
     1681 cggctcgctc atcgtccgtc tatagcacgc ggctcaggcg tcgacgcaga actgcgggat
     1741 gccgcgaccc tcgcagtaga tcgatccgac gaccgcgtcc gggtcgccgg tgaagtcgcc
     1801 gtcgagcacg agatcgcaga cctcgacctc ggtcgcgcca cccttcgggt tctggtcgag
     1861 gtagatgagc tcgcacacca tgtcgcactc gagctgggta tggtcgatca cgcggctgtc
     1921 gcggacgagg agctcgacgg cgcgctcgtc gagctcctcg tcgagcgaga agcccttgct
     1981 cggctgatcg cagttgtcga cccggtagcc gcatgcggcg gtgagggtcg ccagggttgc
     2041 gatcagcagg agtcgcgtgc gcatcgacgc atggtgcagc gaggtcgcgg tgcggcgcaa
     2101 ggggtgatgc gcgcggggcc aggagagcgc tgcttgccgt gcttgccgtg cttgccgtgc
     2161 ttgccggttc atcccagctc caggcccatc gcgtgcaggg cggcgatcgg gtgatgcagg
     2221 gctcgcggga gcgggcgggt ctcggcgtcg ccgggcgggc cgcgccggtc gagctcgctg
     2281 agcgcctcga acagccggtt gccgaccgcg agcgtgcgca gggagacata cgggcggccc
     2341 gcgacgtcgg tggccggcgc cggtcggtcc tcgttggcga acagcatgtg gatcgcctcg
     2401 cccagaaatt tgtgctcctc ggcgacgatg acgaccggcg cggcgagctc gagcagtcgc
     2461 ttgagccgat tgacgcgatt gatcacccgc cggtacaggt cattgatatc ggaggtgacc
     2521 cagcgatagt cgtcgaggcg cttgatcggc cgcagatatg gcggcagcac ccgcaatacg
     2581 cgcaatagca gggccgcgcc gtcggcggtg cgcgcgagca gttcgccgag ccggggccac
     2641 tgggccgggt cggtgtacgc gacgagtcgg tcgagctcgt cgcgcgagat cccggcccgc
     2701 gcggcgacct cgtcgggggc gtgcgcgagc gcgagcggat gcacgaccgg cgccggcagc
     2761 tcgatccgcc cgaagcgcgt cgcccgggcc tcggtgaccg gctcgtcgtc ggcgaagggc
     2821 ccgcgctgga gcggaccctc gccgaagatc tggtcgcaga acagcccgcc gggctcgggc
     2881 gcgagggtcc gctcgcgcaa ggtctccggc ttcttgacct cgcgggtgcg tagccgcaac
     2941 accgcctcgt cgacccgtga cagcgcggtg gtcgagagga agaagccggg cggcggacgg
     3001 agcacgtcga gccagaggtc gccgggtggg cgtgacacgg ctgggatatt accgccaaac
     3061 agcgcggccc gtcgcgtccc tgcggcgggc ccgcacgtgt ggggattgtc ggcacttcgg
     3121 ggcgagggcc caggtatcac ggcctgcgcg gcgctcgctg tggcgcgccg ctcgaacgca
     3181 tgcacgaaag ctggcatgac ccgcgtgatg accctcccgt ttgtcctcgc tgtcgcgctg
     3241 ctcggcgcgt gcggaaccaa ggaagccccg gcgaacgacg cctcgcgtgg caaggccgac
     3301 gccgcgaccg ccggtggggt cgggatcccg ccggtgaccg gtacgccgcg cggcccgcag
     3361 ggcgtcgaga ccccgccggc gccgcccaac ggcggcaacg cccctccggg ggaccgcaac
     3421 agcggggtcg gcacgatcgt cggcaccgac gaggcgaagg cggccgacgc caagtcggcc
     3481 gacggctcga cgaagcagga gcgtcatgcc cagtagtcag tagcgcgggc ggcggctcag
     3541 gcgaggacct cgcgctcgat cgcgcgatac aacttgaggc tcccggcgct cgcggcgtgc
     3601 gcgatggcct cgaggacccg caccgcgcgg tcgagccgga catttttccg cagctcgggc
     3661 cagatccaca tcatctgcag ggccccgttc tcggtcgcgg tcgacagcag ggcgccgcgg
     3721 ccctgcgccg tcgcgggcct ggagagcgtc gggaagccgg cggcgcgcag ctgcatttcg
     3781 agctcgggcc agacccactc cgcgagcgtg ccctcgaaga ctgcggggcg ggcgagcata
     3841 tgcgcgacgc ggacccgcct atcgcaccac aaggtcatct cgacgtgacc ggcacggtcg
     3901 tcccccgggg cgccgtaggc ttcgcggtaa cgcagcgtcg tccacgagct ggtcatacgt
     3961 gggggtgcat cctgctctga aaaatagtag aaaaatcgcg cgcgttgacc gcttctgcgg
     4021 cgagtcgacg cggctatgcg gtgcatcacg aatgcttgta tgtgccggcg ccccgggcgc
     4081 taagaggaat acgactagcg agagatttgt gtggatcgcg gccaccgacc cgtgagatat
     4141 gggaggggat ggaagctgtt cacgaggtgc tggccgaacg ctccgaggaa gtcatgctgc
     4201 gctggaaggc gaccgtgcgc gggacgctcg cgccggagtc gatgccggtg gtcgagctgg
     4261 tcgaccacct gccgatgttc ctgcgcgagg tgatcgtcgc cctgcgagag gaccgcgggc
     4321 tgacggcgac gtatcagccg cagacggagg cgagcaggac cgcggcggag cacggcgagc
     4381 agcgcctgcg cctcggcttc agcctcgact cggtggtgcg cgagtacggg gtgctgcgcg
     4441 acgcgatcgt cgcgacggtg cgcgacgccg ggatcgacgt gtcgtttcgc gagctgcaga
     4501 tcgtgttcga cgcgatcatc gacggcatcg cccacgcggt ctccgagtac acgcgccagc
     4561 gcgacgcgga gctgttccgc cagcacaacg agcacgtcgc gttcatggcc cacgagctgc
     4621 gcaacccgct cggctcggcg cgcatggccg tcgcgctgct caagcgcggc gggcacatcc
     4681 ccgcggacgc gcgcggggcg gcggccctcg accgcggcct gcagcgcatg caggagggca
     4741 tcgaccactc gctgacggtc gcgcgcacgg cctcgggcat cgcggtgcgc cgcgagaaga
     4801 cgcgcctgcg cacattgctg gaagatgtcg agatcggtgc gctcggcgag gcccaggaca
     4861 agggcatcga cctgcgcctc cacctcgagc aagacgccga gatcgaggtc gacgtgcgcc
     4921 tgatctcctc ggcgctcggc aacctggtgc gcaacgcggt gaagtacagc tgcgccagcg
     4981 gctcggtcga gatccgcggc ggcatctcgg tgatcggcgt gcgcatcgag gtcgaggaca
     5041 gctgcggcgg tc
//
LOCUS       DASWNJ010000486         2459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_80199, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000486 DASWNJ010000000
VERSION     DASWNJ010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80199"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VGB85_08310"
     CDS             complement(<1..235)
                     /locus_tag="VGB85_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEY0134068.1"
                     /translation="MNNERRVVITGLGINTPIGDDLETFYQNLLAGKSAITRWKWMKN
                     ENVYSKVGGDLSEYDVKAKLARLRDKLPADMHKR"
     gene            complement(254..862)
                     /locus_tag="VGB85_08315"
     CDS             complement(254..862)
                     /locus_tag="VGB85_08315"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HEY0134069.1"
                     /translation="MAVGVTGQGSAPRPGTIEALGGNFDPSRDLEALVKTYRRRPVFT
                     PGEGTTTVEFGRAEIERMLPHREPFLLVDSVQQVDLGQSAIAGRLTIDPQDPVFRGHF
                     PGDPVYPGVLQVEAIGQCGIVLQNLISRDSTSVGEDVRPRPLRLLKVHHALFQGAVRP
                     GEWVTLLARSLDQSDYVSTCIGQVVHGDEVKALAVFEVYLLD"
     gene            complement(847..1863)
                     /locus_tag="VGB85_08320"
     CDS             complement(847..1863)
                     /locus_tag="VGB85_08320"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY0134070.1"
                     /translation="MTLVFMFPGQSSRYPGMLDKLRRLHTRADEVLALASDTLGRDLR
                     SHYSEDNGAAFDRNVDVQIGVFVANHMMLSILQAEGIDAELSLGLSLGEYNHLTHIGA
                     IDFVDALRVVKARGEAYDAGPRGWMASVQPLDLDTLEEVVEAVRARGLGVLEIVNLNS
                     PRQHVLSGDQAAIEAAVAILEDEHYVVPTIIERQVPMHSSRFAPVGEALLPHLRRARF
                     RAARRPYLPNRLGSPVPSASHDEYVTMLADHVHTPVLWRRSIDHIIAAHPDAVLVEVG
                     PRQVLFNLLDRKWVKSPRYCTDAREDAPAHLAGVIAALRAHNPLTVPVPQSVSHEVRE
                     WPSA"
     gene            complement(1860..>2459)
                     /locus_tag="VGB85_08325"
     CDS             complement(1860..>2459)
                     /locus_tag="VGB85_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0134071.1"
                     /translation="AEEHGVGVLIDEAHSILVYGARGRGVIEHFGAESRVAVQFATFS
                     KAFAGAGSFTVGSKKLLQYIRYYINAYGFSCALPPSVIAGVSKALDIATRDNSLREKL
                     WENTRYFREQALGLGLNLGHSTSQVVPIIIGSDRRRLYELARDMHVKGLFLAPVDYPS
                     VPEDGLRFRVAITAAHTREDLDLALQILEDTVVRTAEQA"
BASE COUNT          369 a          766 c          937 g          387 t
ORIGIN      
        1 cccgcttgtg catgtcggcg gggagcttgt cgcgcaggcg ggcgagcttg gccttgacgt
       61 cgtactccga gaggtcgccg ccgaccttgg agtagacgtt ctcgttcttc atccacttcc
      121 agcgggtgat cgccgacttg ccggcgagga ggttctgata aaaggtctcg agatcgtccc
      181 cgatcggcgt gttgatgccg agtccggtga tgacgacgcg tcgttcgttg ttcatggcaa
      241 tgcgctctcc gggtcagtcg aggaggtaga cctcgaacac ggcgagggcc ttcacctcgt
      301 cgccgtggac gacctgacca atacatgtgg aaacgtagtc gctctggtcg agcgatcgag
      361 ccagcagggt cacccactcg ccggggcgga ccgcgccctg aaacagcgcg tggtggacct
      421 tgagcaggcg cagcggccgc gggcgaacgt cctcgccgac gctcgtgctg tcgcggctga
      481 tcaggttctg cagcacaatg ccgcattgac caatcgcctc gacctgcagc acgcccgggt
      541 acaccgggtc accggggaag tggccgcgga acacggggtc ctgcgggtcg atcgtcaggc
      601 ggccggcgat cgcgctctga ccgaggtcga cctgttgcac gctgtcgacc agcaggaacg
      661 gctcgcggtg cgggagcatg cgctcgatct ccgcgcggcc gaactcgacg gtcgtggtgc
      721 cctcgccggg cgtgaacacc gggcgacggc ggtaggtctt gaccagcgcc tcgaggtcgc
      781 gtgacggatc gaagttgccg ccgagcgcct cgatggtccc cggtcgcggg gcgctgccct
      841 gccctgtcac gccgacggcc attcgcggac ctcgtgcgag acgctctggg gcacgggcac
      901 ggtcaggggg ttgtgcgcgc gcagggccgc gatcacgccg gcgaggtggg cgggcgcgtc
      961 ctcgcgggcg tccgtgcagt agcgcgggct cttgacccac ttgcggtcga gcaggttgaa
     1021 cagcacctgg cgcgggccga cctcgacgag cacggcgtcc gggtgggcgg cgatgatgtg
     1081 gtcgatcgac cggcgccaca gcacgggggt gtgcacgtgg tcggcgagca tcgtcacgta
     1141 ctcgtcgtgg ctggccgaag ggacaggtga gccgaggcgg ttcgggaggt agggccggcg
     1201 cgcggcgcgg aagcgggcgc gacgcaggtg cggcagcagg gcctcgccga cgggggcgaa
     1261 gcgcgacgag tgcatcggca cctggcgctc gatgatcgtc gggacgacgt agtgctcgtc
     1321 ctcgaggatc gcgacggcgg cctcgatggc cgcttgatcg ccggacagca cgtgctggcg
     1381 cggggagttg aggttgacga tctcgagcac gccgaggccg cgggcgcgca cggcctcgac
     1441 gacctcctcg agggtgtcga ggtcgagcgg ctgcaccgag gccatccagc cgcgcgggcc
     1501 ggcgtcgtag gcctcgccgc gagctttcac gacgcgcagc gcgtcgacga agtcaatcgc
     1561 gccgatgtgc gtgaggtggt tgtactcgcc gaggctgagg cccagcgaca gctcggcgtc
     1621 gatgccctcg gcctggagga tcgagagcat catgtggttg gcgacgaaca cgccgatctg
     1681 gacgtcgacg ttgcggtcga aggcggcgcc gttgtcctcg ctgtagtggc tccgcaggtc
     1741 gcggccgagc gtgtcggacg cgagcgcgag cacttcatcg gcgcgggtgt gcagccgccg
     1801 gagtttgtcc agcatgccgg ggtaccggga gctctgcccg gggaacatga agacgagggt
     1861 catgcctgct ccgcagtacg aaccacggtg tcctcgagga tctggagggc gaggtcgagg
     1921 tcctcacgcg tgtgcgccgc cgtgatcgcg acgcgaaagc gcagaccgtc ctcgggcacc
     1981 gaggggtagt cgacgggggc caggaacagg cccttgacgt gcatgtcacg cgccagctcg
     2041 tagaggcggc ggcggtccga gccgatgatg atcgggacga cctggctggt cgagtggccg
     2101 aggttgaggc cgaggccgag cgcctgctcg cggaagtagc gggtgttttc ccacagcttc
     2161 tcgcgcagcg agttgtcgcg ggtggcgatg tcgagcgcct tgctgacgcc ggcgatcacc
     2221 gacggcggca gcgcgcagct gaagccgtac gcgttgatgt agtagcggat gtactgcagc
     2281 agcttcttcg agccgacggt gaagctgccg gcgccggcga aggccttgga gaaggtggcg
     2341 aactgcacgg cgacccgcga ctcggcgccg aagtgctcga tgacgccgcg gccgcgcgcg
     2401 ccgtagacga ggatcgagtg cgcctcgtcg atcaggacgc cgacgccgtg ctcctcggc
//
LOCUS       DASWNJ010000487         4049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_608944, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000487 DASWNJ010000000
VERSION     DASWNJ010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608944"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VGB85_08330"
     CDS             <1..220
                     /locus_tag="VGB85_08330"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEY0134072.1"
                     /translation="SLLERLLTASCPDGGVVLDPFNGSGTTGVAAVKLGHSYIGVDLE
                     TEYLELTTNRLLEVSPELRERPALRVVV"
     gene            complement(222..1103)
                     /locus_tag="VGB85_08335"
     CDS             complement(222..1103)
                     /locus_tag="VGB85_08335"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY0134073.1"
                     /translation="MSRRLLIALVCLIVLAVLLTWCGRPAPISIGTFNIRTFPGERTD
                     PEAVAAAIAGLDADVIAVQEIADPKAFQRVLDLASAKSGRYYEMTLRHSHCRLDSMEI
                     GVVHDAQRLELVESSLLGDDPSCPKGQAPGLVALLRTTDGRRLAFASVHFKAGGGAKS
                     WSERQAQWATLAAEIPDVSERLQAPVIVAGDFNSTGYLDADSHERRFIDALVAHEQLQ
                     LPTAALGCSMYWQRRRGAAYEASLLDHVLAQAGPRFSAPEALGMCAALACAPQPSAPR
                     DFDAISDHCPVRVEVRL"
     gene            1233..1637
                     /locus_tag="VGB85_08340"
     CDS             1233..1637
                     /locus_tag="VGB85_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134074.1"
                     /translation="MNRSRRLSALRARAERGSTEGERAAARRALEVEEQRPARAEHHP
                     ELVRADVEVGEVEPGEWPARDRDGRGSSYVVVVPNADTAREWFRRGAMCILSAGGRRF
                     VAKASGYSLPNNIGGPAPLAYFVRWRDLPRPD"
     gene            complement(1678..2130)
                     /locus_tag="VGB85_08345"
     CDS             complement(1678..2130)
                     /locus_tag="VGB85_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134075.1"
                     /translation="MSQEWLYRASGALEGPDVTRELATEFGFICRTAYTEGTENLIAH
                     VASVDFGDIIHLYFAGPASGTLLGAFRVVGPVKHPHPEFFGKAVHKTKLRTVLDGPLK
                     DYLAEKATGYTADPTLKLFTGWPIVPEETRSPSYIKEMFPGRGALVKR"
     gene            2427..>4049
                     /locus_tag="VGB85_08350"
     CDS             2427..>4049
                     /locus_tag="VGB85_08350"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative inorganic carbon transporter subunit
                     DabA"
                     /protein_id="HEY0134076.1"
                     /translation="MSKTAASAPPNPRVRLRKTLERIAHYLPAQGPLEVFVHHNTLHA
                     YQHLPFHEAIAAAQAQLGVHGYLPEEKYREALATGRIAATDLEAVIAEATFATTRVAP
                     GFPPERTVARLVVLHSIAAESPASLHWQIVEKQANSQFAADVPVAARRAIVTATDMWL
                     APRMAGQAEVDDGHSKLFRLADKSLSADEEVARLIVGQPEFLSPAAELAALLGARARP
                     EAFMARHEAVALRSLWTACVDACRHLEGTVRREDDAPLLPREALLSVSDEDADDLVHA
                     TLVPLCAAFLDRGQSHWSMPDRELGFFRAWLGVMLTGRSVRPGWLSELGPRLAKYQER
                     RVEGEDVVLELFAELGIPEAEQERFIERTLLRLPGFAGMFARLETAPGPIGRSRARVN
                     LIDFLAVRLLLDLFAFQDIARRLGHHGPVAEIRRFCAHLPRIAPPRMRGPHDTAWPLF
                     MLAQHAGVAAPTIRQAQREHIDAVIALLDRLDDHARLILWHEAYERRYRNQLLDAVAA
                     NIHGEELPPAPAAPPRFQAMFCIDDRFESSRRH"
BASE COUNT          559 a         1511 c         1398 g          581 t
ORIGIN      
        1 gtcgctgctc gagcgcctgc tgaccgcctc gtgcccggac ggcggcgtcg tgctcgaccc
       61 cttcaacggc agcggcacca ccggcgtcgc cgcggtgaag ctcggccaca gctacatcgg
      121 cgtcgacctc gagaccgagt acctcgagct gacgaccaac cgcctgctcg aggtctcccc
      181 cgagctgcgc gagcgcccgg ccctgcgcgt cgtcgtctga gtcacagccg gacctcgacg
      241 cgcaccggac agtgatccga gatcgcgtcg aagtcccgcg gcgcggaggg ctgcggcgcg
      301 caagccagcg cagcgcacat gccgagcgcc tccggggccg agaaccgcgg gcccgcttgc
      361 gcgagcacgt ggtcgagcag gctcgcctcg taggccgcgc ctctgcgtcg ttgccagtac
      421 atcgagcagc cgagcgccgc ggtcggcagc tgcaattgtt cgtgggcgac cagcgcgtcg
      481 atgaagcggc gctcgtggct gtcggcgtcg aggtagccgg tactattaaa atcgccggcg
      541 acgatcaccg gggcctgcag gcgctcgctc acgtcgggga tctcggcggc gagcgtcgcc
      601 cactgcgcct ggcgctcgga ccacgacttc gccccgccgc cggccttgaa gtggaccgac
      661 gcgaacgcga gccggcggcc gtcggtcgtc cgcagcagcg cgacgaggcc gggcgcctgg
      721 cccttcgggc agctcgggtc gtcgccgagc aggctgctct cgaccagctc gagccgctgc
      781 gcgtcgtgga ccacaccgat ctccatcgag tcgagccggc agtgcgagtg gcgcagcgtc
      841 atctcgtagt agcgcccgga cttcgccgag gcgaggtcga gcactcgttg aaatgccttc
      901 gggtcggcga tctcctgcac cgcgatgacg tccgcgtcga gcccggcgat cgccgcggcg
      961 accgcctccg ggtcggtgcg ctcgcccggg aaggtccgta tattgaaagt cccgatcgag
     1021 atcggcgccg gccgcccgca ccacgtcagc agcacggcga gcacgatcag gcagaccaag
     1081 gcgatcaaca ggcggcgcga cacgtccgcc agcgtggtgc gagcccgcgc acgctgtcaa
     1141 gcggcggcca gcgcggccag caagcacccg acgacgcgcc ggcgagccgc ccgcactggc
     1201 acgccaactt cctcccgggg tagcttcacg ccgtgaatcg cagccgccga ctctccgccc
     1261 tgcgtgcccg cgccgagcgc ggctccaccg agggtgagcg cgccgcggcc aggcgcgcgc
     1321 tcgaggtcga ggagcaacgg cccgcgcgcg ccgagcacca cccggagctc gtgcgcgccg
     1381 acgtcgaggt cggtgaggtc gagcctggcg agtggccggc gcgcgaccgc gacgggcgcg
     1441 ggtcgagcta cgtggtcgtc gtgccgaacg ccgacacggc ccgcgagtgg tttcgtcgcg
     1501 gggcgatgtg cattctcagc gccggcggtc ggcgcttcgt ggcgaaggcg tccggttatt
     1561 cgctgccgaa caacatcggc gggccggccc cgctggcgta cttcgtacgc tggcgcgacc
     1621 tgccccgccc cgactgatcc agctgatcca gctgatccag ctgatccagc ggacgcttta
     1681 gcgcttcacg agcgcgccgc ggccggggaa catctccttg atgtaactcg gcgagcgcgt
     1741 ctcctccggc acgatcggcc agccggtgaa cagcttgagc gtcgggtccg ccgtgtagcc
     1801 ggtcgctttc tccgcgagat agtccttgag cggaccgtcg aggaccgtgc gcagcttggt
     1861 cttgtgcacc gccttcccga agaactccgg gtgcgggtgc ttgacgggcc cgacgacgcg
     1921 gaacgccccg agcagggtgc cgctggccgg gccggcgaag tacaggtgga tgatgtcgcc
     1981 gaagtcgacg ctggcgacgt gggcgatcaa gttctcggtg ccctcggtgt aggcggtccg
     2041 gcagatgaac ccgaactcgg tggcgagctc acgggtgacg tctggcccct ccagggcgcc
     2101 gctcgctcga tatagccact cttgactcat gcaggtctcg ctccgcgacc cttatacgcg
     2161 ctccagcggt ccggctgtag tccgcgaacc ccgcacatgt gggttcgagc gcgagcccgc
     2221 gtcccgagat ctgtctcgaa agacatggcc gatccgacat gtggcgccgg gccgccacgc
     2281 cagacctgcg acgcggcatc gcgggttccg cgccacaggt ggatcaccct acatcctgga
     2341 cacttgcgtc ccgccttgcc cggtccgaga atcccgggcc cggggacata cgaagcagag
     2401 ccgggtcgcc cgtccattcg agtgtgatgt ccaagactgc cgccagcgct ccgccgaacc
     2461 cgagagtgcg cctccgcaag acgctcgagc gaatcgcgca ttacttgccg gcgcaggggc
     2521 ccctcgaggt gttcgtccat cacaacaccc tgcacgcgta ccagcacctg ccgtttcacg
     2581 aggcgatcgc cgccgcgcag gcgcagctcg gggtgcacgg ctacctgccg gaggagaagt
     2641 accgcgaggc gctggcgacc gggcggatcg ccgcgaccga cctcgaggcc gtgatcgcgg
     2701 aggcgacctt cgcgacgacg cgggtcgccc cgggcttccc acccgagcgg acggtcgcgc
     2761 ggctggtcgt gctgcactcc atcgccgccg agtcgccggc ctcgctgcac tggcagatcg
     2821 tcgagaagca ggccaacagt caattcgcgg cggacgtgcc cgtcgcggcc cggcgcgcga
     2881 tcgtcacggc gaccgacatg tggctcgcgc cgcgcatggc cggccaggcc gaggtcgacg
     2941 acggccactc gaagctgttt cgcctcgccg acaagtcgct ctccgccgac gaggaagtcg
     3001 cgcgcctgat cgtcggtcag ccggaattcc tgtcccccgc ggccgagctc gccgccctgc
     3061 tcggcgcgcg cgcgcggccc gaggccttca tggcccgcca cgaggccgtc gccctgcgct
     3121 cgctgtggac cgcctgcgtc gacgcctgcc gccacctcga gggcaccgtg cgccgcgagg
     3181 acgacgcacc cctgctcccg cgcgaggccc tgctgtcggt cagcgacgag gacgccgacg
     3241 acctcgtgca cgcgaccctg gtcccgctgt gcgccgcctt cctcgaccgc ggccagtcgc
     3301 actggtcgat gccggaccgc gagctcggct tctttcgcgc ctggctcggc gtgatgttga
     3361 cgggccgctc ggtgcgcccc gggtggttgt cggagctcgg cccgcgcctg gcgaagtatc
     3421 aggagcggcg ggtcgagggc gaggacgtcg tgctcgagct gttcgccgag ctcggcatcc
     3481 ccgaggccga gcaggagcgc ttcatcgagc gcacgctgct gcgcctgccg ggcttcgcgg
     3541 ggatgttcgc gcggctcgag acggcgcccg gcccgatcgg tcgctcgcgg gcgcgggtca
     3601 acctgatcga cttcctggcg gtgcggctgc tgctcgacct gttcgccttc caggacatcg
     3661 cgcgccggct cggccaccac ggcccggtcg cggagatccg ccgcttttgc gcccacctgc
     3721 cgcggatcgc cccgccgcgg atgcgcgggc cccacgacac cgcgtggccg ctgttcatgc
     3781 tcgcgcagca cgccggcgtc gccgcgccga cgatccgcca ggcccagcgc gagcacatcg
     3841 acgccgtcat cgcgctgctc gaccggctcg acgaccacgc ccgcctgatc ctgtggcacg
     3901 aggcctacga gcgccgctat cgcaaccagc tgctcgacgc cgtcgccgcc aacatccacg
     3961 gcgaggagct gccgcccgcg cccgccgccc cgccgcgctt tcaggcgatg ttctgcatcg
     4021 acgaccgctt cgagtcgtcg cgccggcac
//
LOCUS       DASWNJ010000488         2447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_395737, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000488 DASWNJ010000000
VERSION     DASWNJ010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395737"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="VGB85_08355"
     CDS             <1..1187
                     /locus_tag="VGB85_08355"
                     /inference="COORDINATES: protein motif:HMM:NF036933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HEY0134077.1"
                     /translation="LIIEGTIAPRQVEVYALSGTQNGEMGFYPGDAGFLGTGIAKGRA
                     FRIESDLPIVATQFNPLGGAKAYTTDASLLLPVHTLGKEYYHLAWEKGLGAGSSMVIV
                     ATQDNTVVTVNSTVNTPAGQNGMPALVAGADVVLPTLKQYDYMQVSAAGQDLSGTKIS
                     ANAPVAVFGGHSCGQVPSADIDFCDHLEEQIFPIDTWGSHYVAARSPPRANEPMVWRV
                     VASKDGTNLKFDPPVSIGASYDGLAAGKLVQFTSDGDFDITSNNPVLVAGYMYGCKST
                     KLPDCPGDPSMVLAVPVVQWLSDYVFLVDVSYTDNNVKLVREKGEEVSVGCFGEVTDW
                     TPVSANYESAVVSFNGDATCMPGTNTATGKSPFSIMVVGESASTSYAYPGGLALKPVN
                     PQ"
     gene            1263..>2447
                     /locus_tag="VGB85_08360"
     CDS             1263..>2447
                     /locus_tag="VGB85_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEY0134078.1"
                     /translation="MALHSKILLGLVLGALAGIAANLGLGPTHALVVGLDRYLAAPLG
                     QVFLRMLSMVVMPLVFASIALGVAGVGDVRKVGRMGAKTLAYFLGTTALATGLGLVLV
                     RIVEPGNHISAAVRDQLVADYSHDAAGSLANATRGVEFGVETIVGIVTRNPLASAVSG
                     DMLGVIFFGLVFGAALSMIRADRAQAMTVWLEALNDVVTRIIDMAMHLAPYGVAGLIF
                     GVTSRFGFALLAPLGLYVGLVLGALLLHAVLTMSIVIRVLIGMSPLQFFRRIRAALIT
                     AFSTSSSSATLPTNILIGEQNLGLPSQVCGFVYPLGATMCMNGTALFEGITLIFLCQV
                     FGVQLDVGQMAVVMGMAVLTSVGAAAVPGGSIPLLVGMLAMFGVPGEGIAVILGVDRI
                     LDM"
BASE COUNT          345 a          944 c          740 g          418 t
ORIGIN      
        1 agctgatcat cgagggtacg atcgccccgc gtcaggtcga ggtctacgcg ctgtcgggca
       61 cgcaaaacgg cgagatgggc ttctacccgg gcgacgccgg cttcctcggc accggcatcg
      121 ccaagggccg cgcctttcgc atcgagagcg acctgccgat cgtcgccacc cagttcaacc
      181 cgctcggcgg cgccaaggcc tacaccaccg acgcctcgct gctgctcccg gtccacaccc
      241 tcggcaagga gtactaccac ctcgcctggg agaagggcct cggcgccggc tcgtcgatgg
      301 tcatcgtcgc gacccaggac aacaccgtcg tcaccgtcaa ctcgaccgtg aacacgcccg
      361 ccggccaaaa cggtatgccc gcgctcgtcg ccggcgccga cgtcgtcctg ccgacgctca
      421 agcagtacga ctacatgcag gtctcggccg ccggccagga cctcagcggc accaagatca
      481 gcgccaacgc cccggtcgcc gtgttcggcg gccacagctg cggccaggtc ccgagcgccg
      541 acatcgactt ctgcgaccac ctcgaggagc agatcttccc gatcgacacc tggggctcac
      601 actacgtcgc cgcccgcagc ccgccccgcg ccaacgagcc gatggtctgg cgcgtcgtcg
      661 ccagcaagga cggcaccaac ctcaagttcg acccccccgt ctcgatcggc gcctcctacg
      721 acggcctcgc cgccggcaag ctggtccagt tcacctcgga cggcgacttc gacatcacca
      781 gcaacaaccc cgtgctcgtc gccggctaca tgtacggctg caagtcgacc aagctgcccg
      841 actgccccgg cgacccctcc atggtcctcg ccgtccccgt cgtgcagtgg ctgtcggact
      901 acgtgttcct ggtcgacgtc tcgtacaccg ataacaacgt caagctggtg cgcgagaagg
      961 gcgaggaggt ctcggtcggc tgcttcggcg aggtcaccga ctggaccccc gtctcggcca
     1021 actacgagtc cgcggtcgtc agcttcaacg gcgacgccac ctgcatgccc ggcaccaaca
     1081 ccgccaccgg caagtcgccg tttagcatca tggtcgtcgg cgagtccgcc agcaccagct
     1141 acgcctaccc cggcggcctc gcgctcaagc cggtcaatcc tcagtgatgc ccgggtgatg
     1201 cccggttgat gccggcggct gcctggccga actcgggttg actcgagtta tcatcgcgcc
     1261 gcatggcgct ccacagcaag atcttgctcg gcctggtgct cggggccctc gccggcatcg
     1321 ccgcgaacct cgggctcggg ccgacccacg cgctcgtcgt cggcctcgac cgctacctcg
     1381 cggcgccgct cggccaggtg ttcctgcgca tgctgtcgat ggtcgtcatg ccgctggtct
     1441 tcgcttcgat cgcgctcggc gtcgccggcg tcggcgatgt ccgcaaggtc gggcgcatgg
     1501 gcgccaagac cctcgcctac ttcctcggca ccaccgcgct cgccaccggc ctcggcctcg
     1561 tgctcgtgcg catcgtcgag cccggcaatc acatctccgc cgccgtccgt gaccagctgg
     1621 tcgccgacta ctcgcacgac gccgccggca gcctcgccaa cgcgacccgc ggcgtcgagt
     1681 tcggcgtcga gacgatcgtc ggcatcgtca cccgcaaccc gctcgcctcg gcggtctcgg
     1741 gcgacatgct cggcgtcatc ttcttcggcc tggtgttcgg cgccgcgctg tcgatgatcc
     1801 gcgccgaccg ggcccaggcg atgaccgtct ggctcgaggc cctcaacgac gtcgtcactc
     1861 gcatcatcga tatggcgatg cacctcgccc cctacggcgt cgccggcctg atcttcggcg
     1921 tcacctcgcg ctttggcttc gccctgctcg ccccgctcgg cctgtacgtc ggcctggtcc
     1981 tcggcgcctt gctgctgcac gccgtgctga cgatgagcat cgtgatccgc gtgttgatcg
     2041 gcatgtcgcc gctgcagttc ttccgcagga tccgcgccgc cctgatcacc gcgtttagca
     2101 cctcgtcgag ctccgccacc ctgccgacca acatcctcat cggcgagcag aacctcggcc
     2161 tgccgtccca ggtgtgcggc ttcgtctatc cgctcggcgc cacgatgtgc atgaacggca
     2221 ccgccctgtt cgagggcatc acgctgatct tcctgtgcca ggtgttcggc gtgcagctcg
     2281 acgtcggcca gatggcggtc gtgatgggca tggccgtgct gacctccgtc ggcgccgccg
     2341 cggtccccgg cgggtcgatc ccgctgttgg tcggcatgct ggcgatgttc ggcgtgccgg
     2401 gcgagggcat cgccgtcatc ctcggcgtcg accgcatcct cgacatg
//
LOCUS       DASWNJ010000489         1629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_539391, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000489 DASWNJ010000000
VERSION     DASWNJ010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539391"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1371
                     /locus_tag="VGB85_08365"
     CDS             <1..1371
                     /locus_tag="VGB85_08365"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HEY0134079.1"
                     /translation="SAALERAIGSSADLAAAAIATKRGFVRAGRLRRPRDVLVEQATV
                     AGLVARVEATRDGALYRSDHFNNLGIAHAQLGDARSARDLFQRSLALRHDLLGEEHPQ
                     VVYAEANLGVALTRSQDFSAAAEALRRALAGAERSLGPDHPHVALVAINLGEAARLLG
                     RFTEAGAHLDRALAIQTALLGPTATDLHYVLAARGALAIDRWDYPAASADYGRVLELL
                     DHGKSEDAAARIDALRGLGLAAAGQGAFDQARSHLEQALALGERSLGADEIWAARLHD
                     ALGSMHLRAGEAELALPRFRRAQAVRLAHLAAGDPLVGESHRRIGEALLASGSLTEAA
                     VELARAREIVEAALPVESLPVAEVHHRLGDLDLARGDRAAAREAFTRAIAIYATISEP
                     DLHQLAFARFGLARALADQAPAEALATADLALAALKRRAAPRPARIADEIAAIEAWVA
                     ARRPRR"
BASE COUNT          189 a          713 c          539 g          188 t
ORIGIN      
        1 agcgccgccc tcgagcgagc gatcggctcc agcgccgacc tcgctgccgc cgcgatcgcc
       61 accaagcggg gcttcgtccg cgccggccgc cttcgacggc cccgcgacgt cctcgtcgag
      121 caggcgaccg tcgccgggct cgtcgcccgc gtcgaggcga cccgcgacgg cgcgctgtac
      181 cgcagcgacc acttcaacaa cctcggcatc gcccacgccc agctcggcga cgcccgcagc
      241 gcccgcgacc tgttccagcg ctcgctcgcg ctgcgtcacg acctgctcgg cgaggagcac
      301 ccccaggtcg tttatgccga ggccaacctc ggcgtcgcgc tgacccgcag ccaggacttc
      361 tccgccgccg ccgaggcgct gcgccgcgcc ctcgccggcg ccgagcgcag cctcgggccc
      421 gaccacccgc acgtcgccct cgtcgcgatc aacctcggcg aggccgcgcg cttgctcggc
      481 cgcttcaccg aggccggcgc ccacctcgac cgcgccctgg cgatccagac cgccctgctc
      541 ggcccgaccg cgaccgacct ccactacgtg ctcgcggcgc gcggcgccct cgccatcgac
      601 cgctgggact accccgccgc cagcgccgac tatggccgtg tgctcgagct gctcgaccac
      661 ggcaagagcg aggacgccgc ggcccgcatc gacgccctgc gcggcctcgg cctcgcggcg
      721 gcgggccagg gcgcgttcga ccaggcccgc agccacctcg agcaggcgct cgcgctcggc
      781 gagcgcagcc tcggcgccga cgagatctgg gccgcccgcc tccacgacgc gctcggctcg
      841 atgcacctgc gggccggcga agccgagctg gcgctccccc gcttccgccg cgcccaggcc
      901 gtccggctgg cccacctcgc ggccggcgat ccactcgtcg gcgagtccca ccgccgcatc
      961 ggcgaggccc tgctggcgag cggctcgctc accgaggccg cggtcgagct cgcgcgcgcc
     1021 cgcgagatcg tcgaggccgc gctgccggtc gagagcctcc cggtcgccga ggtccatcac
     1081 cgcctcggcg acctcgacct cgcgcgcggc gaccgggccg ccgcccgcga ggcgttcacg
     1141 cgagcgatcg ccatctacgc cacgatcagc gagcccgacc tccaccagct cgcctttgcc
     1201 cgcttcggcc tcgctcgcgc cctcgccgac caggcccccg ccgaggcact cgccaccgcc
     1261 gacctcgccc tcgccgcctt gaagcgccgc gccgcccccc ggcccgcccg catcgccgac
     1321 gagatcgccg cgatcgaggc ctgggtcgcg gcccgccgcc cgcgccgctg acggcggaga
     1381 cgagcacgtg ccgtcgcacg atccagcatt ccggggccag gagctggccc ttcggccgcg
     1441 ccgtctttcg ccgcgagtcc gcgtctcgct ccacgcattg aacagccgcg agcaccacga
     1501 tatcgcgcgg cgcgagcggc ggaggtggac cgctcgcgct ggcggtcgct gggctcgcgt
     1561 ggcaccgcga ggtcaccggg cggccgcgcc gcgtggtgtc ttgcgcgctg tgatccgccg
     1621 aacatgaag
//
LOCUS       DASWNJ010000490         2391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_9277, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000490 DASWNJ010000000
VERSION     DASWNJ010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9277"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1205)
                     /locus_tag="VGB85_08370"
     CDS             complement(42..1205)
                     /locus_tag="VGB85_08370"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0134080.1"
                     /translation="MRNNAPRPPGTSSRRLLCAGLIAAACFVEENGELVKHTLALPTG
                     ITLKYVELGDPAGEPVIFLHGYTDTARSFHPTAQALAKLSKKGTPRRLFVLDLRGHGG
                     SSMPGDAACPGAPETCFTPGDMADDVLAFMDLVGLASAHLVGHSMGSFVAQDLALDHP
                     DRVDSLVLIGTGSKLTGNVVLQDYILAEPVEGSWKAGFIAQGVDFPAGAYLQTPDDSG
                     ADALAWIEGSWVVEPAADPDFLAAIVPETAATRMGTWVGAARALLATDHSERLEDLEV
                     PTLIMWATQDAIFTLDDQEQLLARLDEAAEDCDSSYVFKVYGKRPLPNSGVPDDIGHN
                     TQWALPQAVAGDIDAFLRTGEPTTDLYYAAADDPQQIVREPGVAELRHSQPCE"
     gene            complement(1256..>2391)
                     /locus_tag="VGB85_08375"
     CDS             complement(1256..>2391)
                     /locus_tag="VGB85_08375"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEY0134081.1"
                     /translation="EFDAGDATATTATTAEPAEGTRAWLTQAGPLLRSGLALAGANER
                     VGRDDGILTALEVASMDLRGTELVVLSACETGVGEVDAGEGVYGLRRALTIAGARAQV
                     MSLWKVDDAATRDLMIAYYKSLAEGTGRGEALRAAQRVVLADAKRRHPYYWAAFIPSG
                     AWSPMQLAPPQVVSGGSQPSSDEPRGVREYWRNPPKKPIFYLGGSYSAPFSVKPFADS
                     TVAQRSGMRLDFLFNVAPRYWTGLTYGRSMWDVGASPTLVNAMESKLGSVELLLGADM
                     LGLPRRWRVRPSLNAWVAGGVAWTRQREREGGGADERAFGLIGTFGADAQLHIRFTPR
                     VDLRLGGGVTRSGLFMKDAVIAGAAKFPAAWRWMAGGAIGFLF"
BASE COUNT          330 a          824 c          909 g          328 t
ORIGIN      
        1 aggccgccgt ggcgcgcgtg ctcgggcggg cgagctgacg atcattcgca gggctgcgag
       61 tggcgcagct cggcgacccc gggctcacga acgatctgct gcgggtcgtc cgcggccgcg
      121 tagtacaggt cggtcgtcgg ctcgccggtg cgcaggaacg cgtcgatgtc gccggcgacc
      181 gcctgcggca gcgcccattg cgtgttgtgg ccgatgtcgt cgggcacccc gctgttcggc
      241 agcggccgct tgccgtagac cttgaacacg tagctgctgt cacagtcctc cgcggcctcg
      301 tcgaggcgcg cgagcagctg ctcctgatcg tcgagcgtga agatcgcgtc ctgtgtggcc
      361 cacatgatca gggtcgggac ctcgaggtcc tcgaggcgct cgctgtggtc ggtggcgagc
      421 agcgcccggg cggcgccgac ccacgtgccc atgcgcgtcg ccgcggtctc gggcacgatc
      481 gcggcgagga agtccgggtc ggccgcgggc tcgacgaccc agctgccctc gatccacgcg
      541 agcgcgtcgg cgccggagtc gtcgggggtc tggagatacg cgcccgcggg gaagtcgacg
      601 ccctgcgcga taaagccggc cttccagctg ccctcgaccg gctcggcgag gatgtagtcc
      661 tgcagcacga cgttgccggt cagcttcgag ccggtgccga tcagcacgag cgagtcgacg
      721 cgatcggggt gatcgagcgc gaggtcctgc gcgacgaagc tgcccatcga gtgaccgacg
      781 aggtgcgcgc tcgccagacc cacgaggtcc atgaaggcca gcacgtcatc ggccatgtcg
      841 ccgggcgtga agcaggtctc gggcgcgccc ggacaggcgg cgtcgccggg catcgaggag
      901 ccgccgtgac cgcgcaggtc gagcacaaac agccgccgcg gcgtgccctt cttcgacagc
      961 ttggccagcg cctgcgcggt cgggtggaac gagcgcgcgg tgtccgtgta gccgtgaagg
     1021 aagatcaccg gctcgcccgc ggggtcgccg agctcgacgt acttgagcgt gatgccggtc
     1081 ggcagggcga gcgtgtgctt caccagctcg ccgttctcct cgacgaagca ggcggccgcg
     1141 atcaggccgg cgcacagcag ccgacgcgac gatgtaccgg gggggcgagg ggcgttgttt
     1201 cgcacgcccc gtccctagcg cagtttcgcg cggaaggaaa gcgcggcggt gcgggtcaga
     1261 acaggaaccc gatcgctccg ccggccatcc agcgccaggc ggccgggaat ttggccgcgc
     1321 cggcgatcac cgcgtccttc atgaacaggc cgctgcgggt cacgccgccg ccgaggcgga
     1381 ggtcgacgcg cggggtgaag cggatgtgga gctgggcgtc ggcgccgaag gtcccgatca
     1441 ggccgaaggc ccgctcgtcg gcgccgccgc cctcacgctc gcgctggcgg gtccaggcga
     1501 cgccgccggc gacccaggcg ttgagggacg gacggacgcg ccagcggcgg ggcaggccga
     1561 gcatgtcggc gccgagcagc agctcgacgc tgccgagctt cgactccatc gcgttgacga
     1621 gggtcggcga ggcgccgacg tcccacatcg accgcccgta ggtgaggccg gtccagtagc
     1681 gcggtgcgac gttgaacagg aagtcgaggc gcatgccgga gcgctgcgcg acggtgctgt
     1741 ccgcgaatgg cttgacggag aacggggcgc tgtagctgcc gccgaggtag aagatcggct
     1801 tcttcggcgg gttgcgccag tactcgcgca cgccgcgggg ctcgtcgctc gacggctgcg
     1861 atccgccgct caccacctgc ggcggcgcga gctgcatcgg gctccaggcg ccggacggga
     1921 tgaaggcggc ccagtagtac gggtgacggc gcttggcgtc cgcgagcacg acgcgctggg
     1981 ccgcacgcag ggcctcgccg cgacctgtcc cttcggccag gctcttgtaa taggcgatca
     2041 tcaggtcgcg ggtcgcggcg tcgtcgacct tccacaggct catcacctgc gcccgcgccc
     2101 ccgcgatcgt cagggcgcgc cggaggccgt agacgccctc gcccgcgtcg acctcgccga
     2161 cgccggtctc gcaggcggag agcacgacga gctcggtgcc ccgcaggtcc atcgaggcga
     2221 cctcgagcgc ggtcaggatg ccgtcgtcgc gaccgacgcg ctcgttggcg ccggcgagcg
     2281 cgagaccgga gcgcagcagc gggccggcct gtgtgagcca ggcgcgggtg ccctccgccg
     2341 gctcggcggt cgtcgcggtc gtcgcggtcg cgtcaccggc gtcgaactcg a
//
LOCUS       DASWNJ010000491         3466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_433070, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000491 DASWNJ010000000
VERSION     DASWNJ010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433070"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VGB85_08380"
     CDS             <1..278
                     /locus_tag="VGB85_08380"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY0134082.1"
                     /translation="GLMQLMPATARELAGSLGERSRPYDPGFNIRAGSLYLSRMIKRF
                     GGDIKLGLAAYHGGPGHVLKWKKAGKRGVPDGSKKYVEKVLAARELF"
     gene            510..1178
                     /locus_tag="VGB85_08385"
     CDS             510..1178
                     /locus_tag="VGB85_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134083.1"
                     /translation="MNCLRWMTFAAVLTGCGSEAVLEESSLRRAAEEYVATYNDWDAA
                     RLSAIFASPPSAEWFHAHFTWTQERIGKCGAPRAMWRVGDRQAGFSYLCERGEMYQYF
                     TLDEHGRLLRTNGGAAGRPAEGALATALDTVLAAMPADASTLASKRWARPLLRDWVVA
                     RGRCELGRTVVVGELSGAFELDCEGKSLTMKLRVNKNGEINSVQLWREPQDYSSVFFK
                     APVS"
     gene            complement(1242..2846)
                     /locus_tag="VGB85_08390"
     CDS             complement(1242..2846)
                     /locus_tag="VGB85_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0134084.1"
                     /translation="MLTVSEVTKSYGGKVLFDDVTTSFEPGHRYGLTGANGAGKSTFM
                     KILAGQLEPDKGKVSRLPRSRLSVLHQDHYRYENDRIRDVVIMGNQKLWSAMSEKERL
                     LAEGSTSDEVGMRLGELENTIAEENGYGAEYEAEQLLEGLGLPASQQEQPLKALTGGM
                     RLRVLLAQALFGSPDILLLDEPTNHLDLESIHWLSNFLMEYRGVLVVVSHDRHFLNEV
                     CTHTADVDYQNIITYTGGYDEMMKQKIQFRISAEKGNASKQKKISELKEFIQRFGANA
                     KKASQAQSRRRAIAKIDLVDLKRSNIARPFIRFDPKVQSGKLALAVDKLSKTFEKPVL
                     KDLIFNATRGEKIAVVGPNGIGKTTLLKCLAGVYEPDRGKITLGHEVGLGYLPQDHDE
                     GIGKADNMTAFQWLYQWDPKSTVEEIRGLLGRMLFPSEDADKPVKALSGGETVRLLLA
                     KLTLTKPNLLLMDEPTNHLDLESIRALTEALQKYEGTLIFVSHDHAMIAEVATQVLEL
                     KGEKGYDLFPGTYEEFLEKTGKNVKR"
     gene            2989..>3466
                     /locus_tag="VGB85_08395"
     CDS             2989..>3466
                     /locus_tag="VGB85_08395"
                     /inference="COORDINATES: protein motif:HMM:NF013158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134085.1"
                     /translation="MTGQPLRHLALGLLVLVAWALPHVARACSCMLPPAPAAAVERAE
                     AVFEARVENATVDFGAQGPGIVRYDLEVLRQWKGELGAAVQLSTRTSAAACGRNLAVG
                     KVYLIYAARQDDGQLTDNLCSRTRLASTADEDLAVLGPGATAAGTRPVAEPTSREPP"
BASE COUNT          494 a         1190 c         1205 g          577 t
ORIGIN      
        1 agggcctcat gcagctgatg ccggccaccg cgcgcgagct ggccggcagc ctcggcgagc
       61 gctccaggcc ctacgacccc ggcttcaaca tccgcgccgg cagcctgtac ctctcgcgca
      121 tgatcaagcg cttcggcggc gacatcaagc tcggcctcgc cgcctaccac ggcggacccg
      181 gccatgtgct caagtggaag aaggccggca agcgcggcgt ccccgacggc agcaagaagt
      241 acgtcgagaa ggtcctcgcc gcgcgcgagc tgttctgacg ccgcgggccc aggaacgaga
      301 ccaggcgcga gaccattccg gcccggccat tccagacggc ccgcgggcgc gccgcagcac
      361 ctgtccccaa agacaggaga gacggcccgg acgtaagcac aggtaaggga gcacgcggac
      421 accggcggcg caagcggccg cgcacccccg gggcgcgtgg ggcctcgcgg gccgtcgaca
      481 gcgccggggc gccctgatac ggtgacgcga tgaattgtct acgctggatg acgttcgctg
      541 cggtgctcac cggttgtggt tcagaggctg tgctcgagga gagctcgctg cgccgtgccg
      601 ccgaggagta cgtcgcgacc tacaacgact gggacgcggc gcgtctgtcc gcgatcttcg
      661 ccagcccgcc gtcggccgag tggttccacg cgcacttcac ctggacgcag gagcgcatcg
      721 gcaagtgcgg cgccccgcgg gccatgtggc gcgtcggcga ccggcaggct ggctttagct
      781 atctgtgcga gcgcggcgag atgtaccagt acttcacgct cgacgagcac ggccgcctgc
      841 tgcgcaccaa cggcggcgcc gcgggtcgcc cggccgaggg cgccctggcg accgcgctgg
      901 acaccgtgct cgcggcgatg ccggccgacg cctcgaccct ggcgtcgaag cggtgggccc
      961 gtccgctgct gcgcgactgg gtcgtcgccc gcggccgctg cgagctgggg cgcaccgtcg
     1021 tcgtcggcga gctcagcggc gccttcgagc tcgactgcga gggcaagtcg ctgacgatga
     1081 agctgcgcgt caacaagaac ggcgagatca acagcgtcca gctgtggcgc gagccgcagg
     1141 actactcgag cgtgttcttc aaggcgcccg tcagctgagc cgtcgctcgg cgaacgcacg
     1201 aagggcccgg cgcgcaacgc cgggcccttt tgcgtgtcgc ttcagcgctt gacgttcttg
     1261 ccggtcttct cgaggaactc ctcgtacgtg ccggggaaca ggtcgtagcc cttctcgccc
     1321 ttgagctcga gcacctgggt ggcgacctcg gcgatcatcg cgtggtcgtg gctgacgaag
     1381 atcagcgtgc cctcgtactt ctgcagggcc tcggtcaggg cgcggatcga ctcgagatcg
     1441 aggtggttgg tcggctcgtc catcagcagc aggttgggct tggtcagcgt cagcttggcg
     1501 agcagcaggc gcacggtctc gccgccgctg agcgccttga ccggcttgtc ggcgtcctcc
     1561 gaggggaaca gcatgcgccc gagcaggccg cggatctcct cgacggtcga cttggggtcc
     1621 cactggtaca gccactggaa ggccgtcatg ttgtcggcct tgccgatgcc ctcgtcgtgg
     1681 tcctgcggca ggtagccgag gccgacctcg tggccgagcg tgatcttgcc gcggtcgggc
     1741 tcgtagacgc cggcgaggca cttgagcagg gtcgtcttgc cgatgccgtt ggggccgacg
     1801 acggcgatct tctcgccgcg ggtcgcgttg aagatcaggt ccttgaggac cggcttctcg
     1861 aaggtcttgc tcaatttatc gaccgcgagc gcgagcttgc cgctctggac cttggggtcg
     1921 aagcggatga acgggcgggc gatgttggat cgcttgaggt cgacgaggtc gatcttggcg
     1981 atcgcgcgcc ggcgtgactg ggcctggctc gccttcttgg cgttggcgcc gaagcgctgg
     2041 atgaactcct tgagctcgga gatcttcttc tgcttgctgg cgttgccctt ctccgcggag
     2101 atccgaaact ggatcttctg cttcatcatc tcgtcgtagc cgccggtgta cgtgatgatg
     2161 ttctggtagt cgacgtccgc ggtgtgggtg cacacctcgt tgaggaagtg gcggtcgtgc
     2221 gagacgacga ccagcacgcc gcggtactcc atcaagaagt tcgacagcca gtggatcgac
     2281 tcgaggtcga ggtggttggt cggctcgtcg agcagcagga tgtccgggct gccgaacagc
     2341 gcctgggcca gcagcacccg caggcgcatg ccgccggtca gcgccttgag cggctgctcc
     2401 tgctgcgacg ccggcaggcc gaggccctcg agcagctgct ccgcctcgta ctccgcgccg
     2461 tagccgttct cctcggcgat cgtgttctcg agctcgccga ggcgcatgcc gacctcgtcg
     2521 ctcgtgctgc cctcggcgag cagccgctcc ttctcggaca tcgccgacca cagcttctgg
     2581 ttacccatga tcacgacgtc gcggatgcgg tcgttctcgt agcggtagtg gtcctggtgg
     2641 agcacgctca ggcggctccg cggcagccgc gagaccttgc ccttgtcggg ctcgagctga
     2701 cccgcgagga tcttcatgaa ggtcgacttg cccgcgccgt tcgccccggt cagaccgtaa
     2761 cggtgcccgg gctcgaagct ggtggtgaca tcgtcgaaca ggaccttgcc gccgtaagac
     2821 ttggtgactt cggaaaccgt gagcatcgct atccctcgcc ctgcgtccgc aaaggcggcg
     2881 ctatttaggg cacttccccg ccgcttggcc agggcccccg cgccgcgctt ccacacccga
     2941 cagcggccga cttgccgccg cgagcccgca gtggttagct tgcagccctt gaccgggcaa
     3001 ccacttcggc acctcgccct cggcctcctc gtcctcgtcg cctgggcgct tcctcacgtc
     3061 gcgcgcgcgt gctcctgcat gctgccgccg gccccggccg ccgccgtcga gcgcgccgag
     3121 gccgtgttcg aggcccgcgt cgagaacgcc accgtggact tcggcgcgca gggcccgggc
     3181 atcgtccgct acgacctcga ggtactgcgc cagtggaagg gggagctcgg cgcggcggtc
     3241 cagctgagca cgcggaccag cgccgcggcg tgcggacgta acctggcggt cggcaaggtc
     3301 tacctcatct acgcggcgcg tcaggacgac ggacagttga ccgacaacct gtgctcacgg
     3361 acacgtctgg cgagcacggc ggacgaggac ctcgcggtgc tcgggcctgg cgcgacggcg
     3421 gcggggaccc gtcccgtcgc cgagccgacc agccgcgagc cgccgc
//
LOCUS       DASWNJ010000492         1775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_291916, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000492 DASWNJ010000000
VERSION     DASWNJ010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291916"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..>1775
                     /locus_tag="VGB85_08400"
     CDS             155..>1775
                     /locus_tag="VGB85_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134086.1"
                     /translation="MKLSSGPDPGDLSPGAAELSERTDDNDLSAGPHELPPGTGELSP
                     RVVLAALLALIGLVLLPQLGFGLVYDDGWTLVTNGFLRVPGELGVLFSPEAVARNIPD
                     AFRPGLVLFDMLSYQLLGLDARWHHALSIALHLGTCAALAMWLRRLGAPMLLWAGTLA
                     LFGLLAIHAEAIAVVSFREDLLAALLALAAAWTASVAVRRARAWPLAIAAALLSLAAC
                     SMKQSAAAMPLLWLLAEALAPWETRRPWRRLLGLAAALIVGAAATLAFTLYLYGDLNP
                     YGAGNLRLYASRVGTGPVLAMSTQIHLSYLQQMLVPVGLAPEYVDAGADWDEPATLLA
                     STGLLALLLYGVSCVRRRPVVALAIVGGFILALPTSNLAAMPNMRADRFMYLPSLAVC
                     LGLAAAALAAGGRLAVRLGRPQLLLVPLAALAVLHGAVLAGTAFTYRSNGQLWATARK
                     SAPGSARAQAASGELLLANLGGAPDQARRDLLLARAEAHCINAERLDPHYELPQLCFA
                     RVAAARKDWVEARRRFAAALAVSPDRNARIFA"
BASE COUNT          182 a          744 c          576 g          273 t
ORIGIN      
        1 cgtcgtgacc gtcgtcggcg ccgtcctggc cctcacctcc tacggcgtcg actacacccg
       61 ctgcggcccg ctcgccccca agcgccggcg ccagaccgcc ccgccgggcc gctgcgccgc
      121 cgaggactcg aagtaacagg cgagccccgc cggcatgaag ctgtcctccg ggccagatcc
      181 cggcgacctg tcccccgggg cagccgagct gtccgagagg acagatgaca acgacctgtc
      241 cgcggggcca catgagctgc cccccgggac aggtgagctg tccccgcgcg tcgtgctcgc
      301 cgccctgctc gcgctgatcg gcctggtgct gctaccgcag ctcggcttcg gcctggtcta
      361 cgacgacggc tggacgctgg tcaccaacgg cttcttgcgg gtcccgggcg agctcggcgt
      421 gctgttctcg cccgaggccg tcgcgcgcaa catcccggac gcgttccgcc ccggcctcgt
      481 gctgttcgac atgctgagct atcaactgct cgggctcgac gcgcgctggc accacgcgct
      541 gtcgatcgcg ctgcacctcg gcacctgcgc ggcgctggcg atgtggctgc gccggctcgg
      601 cgcgccgatg ctgctatggg ccggcaccct ggcgctgttc ggcctgctgg cgatccacgc
      661 cgaggcgatc gccgtcgtct cgttccgcga ggacctgctc gccgccctgc tcgcgctcgc
      721 ggccgcctgg accgcgagcg tcgcggtccg tcgcgcccgc gcgtggccgc tggcgatcgc
      781 cgccgccttg ctgtctctcg cggcgtgttc gatgaagcag tcggccgccg cgatgccgct
      841 gctgtggctg ctcgccgagg cgctcgcccc ctgggagacc cgccgcccgt ggcgacgcct
      901 gctcggcctc gccgccgcgc tgatcgtcgg cgccgcggcg acgctggcct tcacgctcta
      961 tctttacggc gacctcaacc cctacggcgc cggcaacctg cgcctctacg ccagccgcgt
     1021 cggcaccgga cccgtgctgg cgatgtcgac gcagatccat ctctcgtatc tgcaacagat
     1081 gctggtgccc gtcggcctcg cgcccgagta cgtcgacgcc ggcgccgact gggacgagcc
     1141 cgccaccctg ctcgccagta ccggcctgct cgcgctgctc ctctatgggg tctcgtgcgt
     1201 ccgccggcgc cccgtcgtcg ccctggcgat cgtcggcggc ttcatcctcg ccctgccgac
     1261 cagcaacctc gcggccatgc ccaacatgcg ggccgatcgc ttcatgtacc tgccgagcct
     1321 cgcggtctgc ctcggcctcg ccgccgccgc cctcgcggcc ggtggcaggc tcgccgtccg
     1381 cctcggccgc ccccagctcc tgctcgtgcc gctcgccgcg ctcgccgtgc tgcacggcgc
     1441 cgtgctcgcc ggcaccgcct tcacctatcg cagcaacggt cagctgtggg ccacggcccg
     1501 caagtccgcc cccggctcgg cccgcgcgca ggcggccagc ggcgagctgc tgctcgccaa
     1561 cctcggcggc gcccccgacc aggcccgccg cgatctcctg ctcgcgcgcg ccgaggccca
     1621 ctgcatcaac gccgagcgcc tcgacccgca ctacgaattg ccgcagctgt gttttgcccg
     1681 ggtcgccgcg gcccgcaagg actgggtcga ggcccggcgg cgcttcgcgg cggccctcgc
     1741 cgtcagcccc gaccgcaacg cccgcatctt cgccg
//
LOCUS       DASWNJ010000493         2315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_256935, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000493 DASWNJ010000000
VERSION     DASWNJ010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256935"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="VGB85_08405"
     CDS             <1..1050
                     /locus_tag="VGB85_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134087.1"
                     /translation="NSDKMFDALHKFGFPNTQALPHRQVVHLRGGTRVYCYQVGQMDS
                     CLAVLGDGETMLNLNDAEADHHDCELLRADLGKISVVLNQFSLAGYGGEHDRDARLPG
                     KARQIIDNMLANHRDLGTELTIPIASFVYFSSVDNSYMNAYANTPRAVADAFDEAGLG
                     LAVLAHGDRLEIGKPWDSGPALQQYDAIYQKLPSLPLDPVPPATLPEIRDALARLSAR
                     LREHYPKLFLRAWLRPVSVEIVDLATTIELDLVTGHLRLTPGAAADLSLYAQPLLFAL
                     QFPFGVQTLGVSARLTVRANASNWRRHRILLSMYNAEVYLRPDELFTRRNWDLLRARL
                     RGGLPQLRYQLARMR"
     gene            complement(1057..2211)
                     /locus_tag="VGB85_08410"
     CDS             complement(1057..2211)
                     /locus_tag="VGB85_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134088.1"
                     /translation="MAHDDDDLDFTALMQRQGVEPVKPAARGSGQLTRPVGGTQANKR
                     GAVAPTVKVQAKQRAPAAVAKAGAPAAVAKAGASAAVAKAGASAARGGASAAVVSAGA
                     AKPVAAGQPGVDAELLAARGELAARQEALARVTAERDELAARVEQLTRERDAALRSRA
                     TAEGAAAALRAEAAAPRTPPEVARISLAEALVARGLTDPGEQAQALAALASTESAPRL
                     ISALETRDIAGLRSALERRLALLCGSADCKAPPGAVAIVVSPARCELCGGSEIQRAAT
                     RFIRACEAAGIARVRFVGGSPNYRTQLEELFPRRGPLTVATTAGDRRVPLSKSKAQQR
                     NDDLVVIWGATELDHATSGAYRAQFGRVETIAHRGIGRMLDLAAERITTG"
BASE COUNT          320 a          959 c          703 g          333 t
ORIGIN      
        1 aactcggaca agatgttcga cgccctgcac aaattcggct tcccgaacac gcaggcgctc
       61 ccgcaccgtc aggtcgtgca cctgcgcggc ggcacccgcg tctattgtta tcaggtcggc
      121 cagatggact cctgcctcgc cgtgctcggc gacggtgaga caatgttaaa tctcaacgac
      181 gccgaggccg accaccacga ctgcgagctc ctccgcgccg acctcggcaa gatcagcgtc
      241 gtgctcaatc agttctcgct cgccggctac ggcggcgagc acgaccgcga cgcccgcctg
      301 cccggcaagg cccggcaaat tatcgacaac atgctcgcaa accaccgtga cctcggcacc
      361 gagctcacga tcccgatcgc cagcttcgta tatttctcaa gtgtcgacaa ttcctatatg
      421 aatgcatacg ctaacacccc ccgcgccgtc gccgacgcat ttgacgaggc cggcctcggg
      481 ctcgccgtgc tcgctcacgg cgatcgcctc gagatcggta aaccgtggga cagcggcccc
      541 gcgttacagc aatacgacgc gatctatcaa aaattgccct ctcttcccct cgacccggtc
      601 ccgcccgcga cgctcccgga gatccgcgac gcgctcgcgc gcctgagcgc gcgcctgcgc
      661 gagcactatc ccaagctgtt cctgcgcgcc tggctgcgcc ccgtcagcgt cgagatcgtc
      721 gacctcgcga ccaccatcga gctcgacctc gtcaccggtc acctgcgcct gacgccgggg
      781 gccgccgcgg acctctcgct gtacgcccag ccgctgctgt tcgccctgca gttcccgttc
      841 ggcgtgcaga ccctcggcgt gtccgcgcgg ctgaccgtac gcgccaacgc gtcgaactgg
      901 cgccgccacc ggatcttgct ctcgatgtac aacgccgagg tctacctgcg tcccgacgag
      961 ctgttcaccc gccgcaactg ggacctgctg cgcgctcgcc tgcgcggcgg cctgccgcag
     1021 ctgcgatacc agctcgcgcg catgcgctga tcgaagtcag ccggtcgtga tgcgctcggc
     1081 cgcgaggtcg agcatgcggc cgatcccccg gtgcgcgatc gtctcgacgc gaccgaactg
     1141 cgcgcggtag gccccgctcg tcgcgtggtc gagctccgtc gcgccccaga tcaccacgag
     1201 atcgtcgttg cgctgctgcg ccttcgattt actcaagggc acccggcggt cgcccgcggt
     1261 cgtcgcgacc gtcaacggcc cgcggcgggg gaacagctcc tcgagctgcg tgcgatagtt
     1321 cggcgagccg ccgacgaagc gcaccctggc gatccccgcc gcctcgcagg cgcggatgaa
     1381 ccgcgtcgcc gcgcgctgga tctccgagcc gccgcacagc tcgcaacgcg cgggcgagac
     1441 cacgatcgcc accgcgccgg gcggcgcctt gcagtcggcg ctgccacaca gcagcgcgag
     1501 gcgccgctcg agcgccgagc gcagccccgc gatgtcacgc gtctcgagcg cggagatcag
     1561 gcgcggcgcc gactcggtgc tcgccagcgc cgcgagggcc tgcgcctgct ccccggggtc
     1621 ggtcaaacca cgggcgacga gcgcctccgc cagcgagatc ctcgccacct ccggcggtgt
     1681 ccgtggcgcc gccgcctcgg cccgcagcgc cgccgcggcc ccctcggccg tcgcccgcga
     1741 gcgcagcgcc gcgtcgcgct cccgcgtcag ctgctcgacc cgcgccgcca gctcgtcgcg
     1801 ctcggccgtc acccgcgcga gcgcctcctg ccgcgccgcg agctccccgc gcgccgcgag
     1861 cagctccgcg tcgaccccgg gctgccccgc cgccaccggc ttcgccgccc ccgccgaaac
     1921 cactgctgct gacgcgcccc ccctcgccgc cgaagcaccc gccttcgcca ctgccgccga
     1981 agcacccgcc ttcgccactg ccgccggagc acccgccttc gccactgccg ccggggcgcg
     2041 ctgcttcgcc tgcaccttca ccgtcggcgc gaccgcccca cgcttgttcg cctgcgtccc
     2101 gcccaccggc cgggtcagct gcccgctgcc acgcgccgcc ggcttcacgg gctcgacgcc
     2161 ctggcgctgc atcaacgccg tgaagtcgag gtcgtcgtcg tcgtgggcca tggctcgccg
     2221 agcgtgacca ccgtggtccg tgcaagcaag catgaagtct actggccctc gatgcccgca
     2281 gacccatccc gggcccccct cacggccctc acacg
//
LOCUS       DASWNJ010000494         2488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_644506, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000494 DASWNJ010000000
VERSION     DASWNJ010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644506"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..>2488
                     /locus_tag="VGB85_08415"
     CDS             65..>2488
                     /locus_tag="VGB85_08415"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEY0134089.1"
                     /translation="MGSSNLSRAALTTGVEWNLRAIHGGHAAEVATIRTRFEKLWDAP
                     ETAALTPEWIEQYAARIPARPDWDPPAPPPTPHPIQRDALAALAQTREDGAPRGLVVL
                     ATGLGKTLLAAFDALQMGARRVLFVAHRDEILTQARRAFAALFPGRSAGFFKNTSRDR
                     ETELLFATVQTLARPQHLSSFAKDHFDLIVIDEFHHAAALSYRRILGYFTPRFFLGLT
                     ATPERGDGTDLLHLCADNVVFRADLAFGIRCGRLVPFVYHGLKDTVDYAAIPWRSGRF
                     DPVALSQALATTTHAQQALRGYTEHAPAGPRRGLWFCASIAHAEFMAAFLRDHGIRAV
                     AVHSEKGGAPRAPSLRDLMIGQLEAITTVDVFNEGIDVPDINVVVLLRPTESRVVFLQ
                     QIGRGLRLPDRSTKPHLVILDFIGNHRTFLARPAALMSLFGTQLDDRAAIRRLHEGNP
                     ELPAGCSVHLDTEVIDLLARLAEVPGDNRLLSAFLQLRDTLGRRPTLTELVSEGYQVG
                     LVKRAFVSWWQFLADRNELSTDEARVFAAWRDELVALEKVRGPASAPWPVLRAWVERG
                     GVTRPIDAAALATASDGDDPARALVAMLPRMLVDRGGAIAMLRAVDAIDAPVLEAMID
                     EVAEAWALAARRSRPIVAVAGGVAVKVIHNGSQSPILVFNRTAATPRGEIEVWVEGEP
                     YVFRFVKTFVNVAATRHGGRNVLPALLRWLFGPDAGKNGMDHRAVIREQDGRYTLIPS
                     LAEHAGPTLPYYSDLAVACGLGDTQHSDADRTTPIRIRGEHQVDPRRHFVVRARSDSM
                     NGGELPIRDG"
BASE COUNT          362 a          838 c          894 g          394 t
ORIGIN      
        1 cggtgcgacg tcgtttcacc cgaaggcgta catattcgtg tccggtgcgg gggccgtcgc
       61 atatgtgggc agtagcaacc tgagccgcgc ggcgctgacg accggcgtcg agtggaacct
      121 gcgcgcgatc cacggcgggc acgcggccga ggtcgcgacg atccggacgc gcttcgagaa
      181 gctgtgggac gcgccggaga ccgccgcgct cacgccggag tggatcgagc aatatgcggc
      241 caggatcccg gcgcgcccgg actgggaccc gcccgcacca ccgccgacac cgcatccgat
      301 ccagcgcgac gccctcgccg cgctcgcgca gacgcgcgag gacggggccc cgcgcggcct
      361 cgtggtgctg gcgactggcc tcggcaagac gctgctggcg gccttcgacg cgctgcagat
      421 gggcgcgcgg cgggtgctgt tcgtcgctca ccgcgacgag atcctcacgc aggcgcggcg
      481 ggccttcgcg gcgctgttcc cgggccgcag cgcgggcttc tttaaaaaca cctcgcgcga
      541 ccgcgagacc gagctgctgt tcgccacggt gcagacgctg gcgcgaccgc agcacctgag
      601 cagctttgcg aaggaccact tcgacctgat cgtgatcgac gaatttcacc acgccgcggc
      661 gctcagctat cgccggatcc tcggatattt caccccgcgg ttcttcctcg ggctcacggc
      721 cacgcccgag cgcggcgacg ggaccgatct gctgcacctg tgcgccgaca acgtggtgtt
      781 ccgcgccgac ctggcgttcg ggatccgctg cggtcgcctg gtgcccttcg tttatcacgg
      841 gctcaaggac accgtggact atgcggcgat cccgtggcgg tcggggcgct tcgaccccgt
      901 cgcgctgagc caggcgctcg ccaccaccac gcacgcgcag caggcgttgc gcggctacac
      961 ggagcacgcc ccggcggggc cccgtcgcgg cctgtggttc tgcgcctcga tcgcccacgc
     1021 ggaattcatg gcggcatttt tgcgtgacca cgggatccgg gcggtcgccg tgcacagcga
     1081 gaagggcggc gccccgcggg ccccgagcct gcgcgacctg atgatcggcc agctcgaggc
     1141 gatcaccacg gtcgacgtat tcaatgaggg catcgatgtc cccgatatca acgtggtcgt
     1201 gctgctgcga ccgaccgagt cgcgggtggt cttcctgcag cagatcggcc gcgggctgcg
     1261 gctgccggat cgatcgacca agccgcacct cgtcatcctc gacttcatcg gcaatcaccg
     1321 cactttcctg gcgaggcccg ctgcgctgat gagcctgttt gggacgcagc tggatgaccg
     1381 cgccgcgatc cggcggctgc atgaggggaa cccggagctg ccggccgggt gcagcgtgca
     1441 cctcgacacc gaggtgatcg atctgctcgc gcggctcgcc gaggtccccg gcgataatcg
     1501 tctgctcagc gcgttcttgc agctgcgtga cacgctcggc cgccggccga ccctgaccga
     1561 gctggtctcg gaggggtatc aggtcgggct ggtgaagcgc gccttcgttt cgtggtggca
     1621 gttcctcgcg gataggaatg agctgagcac tgacgaggcg cgcgtgttcg cggcgtggcg
     1681 agacgagctg gtcgcgctcg agaaggtgcg cgggcccgcg tccgcgccat ggcccgtgct
     1741 gcgggcctgg gtcgagcggg gcggggtgac ccggccgatc gacgcagccg cacttgcgac
     1801 ggcgagcgac ggcgacgacc cggccagggc acttgtggcg atgttgccgc gaatgttggt
     1861 agatcgaggt ggcgcgatcg cgatgctgcg cgccgtcgac gcgatcgatg cacccgtgct
     1921 cgaggccatg atcgacgagg tcgcggaggc gtgggcgctc gccgcccgac gctcgcgtcc
     1981 gatcgtggca gtggccgggg gcgtcgctgt gaaggtgatt cataatggca gccagagccc
     2041 gatcctcgtg ttcaaccgca ccgcagcgac gccgcgcggc gagatcgagg tgtgggtcga
     2101 gggcgagccc tacgtgtttc gtttcgtgaa gaccttcgtc aacgtggcgg ccacccgcca
     2161 cggtgggcgt aacgtgctcc cggcgctgct caggtggctg ttcggacccg acgcgggcaa
     2221 aaacggtatg gaccaccggg ctgtgatccg cgagcaggat gggcgctata cgctgatccc
     2281 gtcgctcgcg gagcatgcgg ggccgacgtt gccgtattac agcgacctcg cggtcgcctg
     2341 cgggctcggc gacacgcagc actcggacgc cgatcggacg acgccgatcc ggatccgtgg
     2401 tgagcatcaa gtcgatccac ggcggcactt cgtggtgcga gcgcgtagtg actcgatgaa
     2461 cggaggagag ctgccgatcc gcgacggc
//
LOCUS       DASWNJ010000495         2627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_395833, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000495 DASWNJ010000000
VERSION     DASWNJ010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395833"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2055
                     /locus_tag="VGB85_08420"
     CDS             <1..2055
                     /locus_tag="VGB85_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0134090.1"
                     /translation="GVVLSRWPDPEQNQVCVVSALRGLIHLETAREQGPLEALLQPRM
                     HKLGEMFPELLSNLRVAGTTCAFDLPDAAAQNAFINQRYPRGFMTYPAGTRTIRFRFS
                     ASWEERHLDDLFARVAEDLRRLHDPEALRWVAEGQQPEIDETVVVRAVSEADWPAIMA
                     IEHEAYEPARRDSEAYLRGAAAAGLGLVACDGGSGEILGFCFGAPIETFRDVSGPDQD
                     DRMGRGDSFYSADITVAGAARARGVGRTLKRAQVQWARERGFSFVSGRNRVGATDAMA
                     ALNRSFGAFPVARLTHQYEGNAEADYYRIPLVPPPAPPEIASRVTDLASGLQAPFGPR
                     PEFMATRELVGPCASRINLSNYVTVDLVHYSELLQQVTPRGTGHMYMTTSRDEMVDKS
                     IRCLRLGRPKGQIAVGLEGGYLGHVTAAARSLSDPRGFGPEFGFFDWPRLPHPAGPGG
                     PEATARALDELVARVGAEVVLGIYAEVVGERSGLVLAGEGARVLADACRRHDIPLVLV
                     ETASGGYRGAAGVWGVDTLPAEVVPDVVLWYPGGQIGHIFVGDRYYIKKPLVLISTWD
                     GDEMSAIRAHEHLRAARRRDLAAAAATLDATLRGSFSASSSVAGARLGGLGLYRSVTL
                     PDEKTADAVYQACRHHGLLLGRGLPDTLIFAPPLDAGALLGGPLRDMLAAALPDAPEH
                     QP"
     gene            2052..>2627
                     /locus_tag="VGB85_08425"
     CDS             2052..>2627
                     /locus_tag="VGB85_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HEY0134091.1"
                     /translation="MTDAFVTSSSSSSSTPSIELAEFDQDEPAARGEAHGELWRAQIH
                     ELAAIRSALAVAKGSFRDQGELEAVAALHLPMLERFSAPLYSELLGIARGAGITPAQA
                     VILNHYTDLRDVPPAVLSQETSDDPGGCTAVYVPGDAGGPVLGQTWDMHGTARPFVRM
                     IRIRPKGSDEETLCFTLTGCLGMTGLSADGVG"
BASE COUNT          326 a          946 c          985 g          370 t
ORIGIN      
        1 ggcgtggtgc tgtcgcgctg gccggacccc gagcagaacc aggtgtgcgt ggtgtcggcg
       61 ctgcgcggcc tgatccacct cgagactgcg cgcgagcagg gcccgctcga ggcgctgttg
      121 cagccgcgga tgcacaagct cggcgagatg ttcccggagc tgctgagcaa cctccgcgtg
      181 gccggcacga cctgcgcctt cgacctgccg gacgcggcgg cccagaacgc cttcatcaat
      241 cagcgttatc cccgcggctt catgacctac ccggcgggca cgcggacgat ccgcttccgc
      301 ttcagcgcga gctgggagga gcggcacctc gacgacctgt tcgcccgcgt cgccgaggac
      361 ctgcgccgcc tgcacgaccc ggaggcgctg cgctgggtgg ccgagggcca gcagccggag
      421 atcgacgaga cggtggtcgt gcgcgcggtg agcgaggccg actggccggc gatcatggcg
      481 atcgagcacg aggcctacga gccggcgcgg cgcgactcgg aggcgtacct gcgcggcgcc
      541 gcggcggccg ggctcgggct cgtcgcgtgc gacggcggca gcggcgagat cctcgggttc
      601 tgcttcggcg cgccgatcga gaccttccgc gacgtctcgg gcccggacca ggacgaccgc
      661 atgggccgcg gcgacagctt ctactcggcc gacatcacgg tcgccggcgc ggcgcgggcc
      721 cgcggggtcg ggcgcacgct caagcgggcg caggtgcagt gggcccgcga gcgcgggttc
      781 tcgttcgtgt ccggccgcaa ccgcgtcggc gcgaccgatg cgatggcggc gctcaaccgc
      841 tcgttcgggg cgttcccggt cgctcgcctg acgcatcagt acgagggcaa cgccgaggcc
      901 gactactacc gcatcccgct ggtcccaccg ccggcgccgc cggagatcgc cagccgcgtc
      961 accgacctcg cctcgggcct gcaggcgccg ttcgggccgc gcccggagtt catggcgacg
     1021 cgcgagctcg tcggtccctg cgcgagccgg atcaacctca gcaactacgt gacggtcgac
     1081 ctcgtgcact acagcgagct gctgcagcag gtgacgccgc gcggcaccgg gcacatgtac
     1141 atgaccacct cgcgcgacga gatggtcgac aagtcgatcc gctgcctgcg gctcggcagg
     1201 cccaaggggc agatcgcggt cggcctcgag ggcgggtacc tcgggcacgt gacggccgcg
     1261 gcgcgctcgc tgtccgaccc gcgcggcttc gggcccgaat tcggtttctt cgactggccg
     1321 cgcctgccgc accccgcggg ccccggcggc ccggaggcga cggcgcgcgc gctcgacgag
     1381 ctcgtcgcgc gggtcggcgc cgaggtcgtg ctcgggatct acgccgaggt cgtcggcgag
     1441 cgctcgggcc tggtgctggc gggcgagggg gcccgggtgc tcgccgatgc gtgccgtcgc
     1501 cacgacatcc cgctcgtgct cgtcgagacc gccagcggtg gttatcgcgg ggcggctggc
     1561 gtgtggggcg tcgacacgct gccggccgag gtcgtgcccg acgtggtgct gtggtacccc
     1621 ggcgggcaga tcggccacat ctttgtcggt gatcgctact acatcaagaa gccgctggtg
     1681 ctgatctcga cctgggacgg cgacgagatg tcggcgatcc gggcccacga gcacctgcgg
     1741 gccgctcgca ggcgcgacct cgcggcggcg gccgcgaccc tcgacgcgac cctgcgcgga
     1801 agcttctccg cctcctcgtc ggtcgccggg gcgcgcctgg gcggcctcgg gctgtatcgc
     1861 agcgtcacgc tgccggacga gaagaccgcc gacgcggtgt accaggcgtg ccggcaccat
     1921 ggcctgctgc tcggccgcgg cctgccggac accctgatct tcgcgccgcc gctcgacgcc
     1981 ggggccttgc tgggcggccc gctgcgcgac atgctggcgg cagcgctgcc cgacgcgccc
     2041 gagcaccagc cgtgaccgac gcgttcgtta catcctcgtc ctcttcctcg tccacgccct
     2101 cgatcgagct cgcggagttt gatcaggacg agccggcggc gcgcggcgag gcccacgggg
     2161 agctgtggcg cgcgcagatc cacgagctcg cggcgatccg cagcgcgctc gcggtcgcca
     2221 agggcagctt tcgcgatcag ggcgagcttg aggccgtcgc ggcgctgcac ttgccgatgc
     2281 tcgagcgctt ctcggcgccg ctgtactcgg agctgctggg catcgcgcgc ggggccggca
     2341 tcacgccggc gcaggcggtg attttgaatc actacaccga cctgcgcgac gtgccgcccg
     2401 cggtgctgtc gcaggagacc tccgacgatc cgggcggctg cacggcggtg tatgtgccgg
     2461 gcgacgcggg cgggccggtg ctcggccaga cctgggacat gcacgggacg gcgcggccgt
     2521 tcgtgcgcat gatccggatc cggccgaagg gcagcgacga ggagacgctg tgcttcacgc
     2581 tgaccggctg cctcggcatg accgggctgt cggccgacgg cgtcggc
//
LOCUS       DASWNJ010000496         3217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_395844, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000496 DASWNJ010000000
VERSION     DASWNJ010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395844"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VGB85_08430"
     CDS             complement(<1..376)
                     /locus_tag="VGB85_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0134092.1"
                     /translation="MLERIGEVVRLARERGQLSLATLADRSGVPLPLLAALEQGAPGI
                     TTTQLDDVAAVLALEPVALLNGREVPSESASVFLRHAPMQDFDGRDEAVLDEVLEQGR
                     SLAGLRNLLGDTVLALQAGVFPQ"
     gene            complement(518..1636)
                     /locus_tag="VGB85_08435"
     CDS             complement(518..1636)
                     /locus_tag="VGB85_08435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SO2930 family diheme c-type cytochrome"
                     /protein_id="HEY0134093.1"
                     /translation="MQRMMMLKRVVLALVVLAAPVTMLVSGCDEGDPCDGMTRCQAEG
                     ALPFKSLAEYDFFVGPMVELRPKTGVVPYTVAAPLWSDQAGKDRFIVLPEGGKIEMRS
                     DDGEWGFPEGTILVKNFFFDHDRRDPKAGAKIIETRLLLREAGNWRAFTYVWNDAQTE
                     ATLLRTGKRVNIEFTDVDGAARTEEYIVPNDDQCSSCHERDDVEHALGLITPQMNRMV
                     ERDGKQVAQLTWLAEQGMFTAPPADLATLPAFIDPYGGEGTLDQRARGYLHGNCAHCH
                     QAGGGAGKSGLVFLASEPDLAKVGVCKVPAAAGAGTGGHAHDIVPGKPDESIVVFRMN
                     SLDPEIKMPELPNRVIDQGGLALIREWIAAMPVDVACQ"
     gene            complement(1705..2919)
                     /locus_tag="VGB85_08440"
     CDS             complement(1705..2919)
                     /locus_tag="VGB85_08440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="parallel beta-helix domain-containing protein"
                     /protein_id="HEY0134094.1"
                     /translation="MGACSGGTEDTAGTDTDTDTDGAALPEGCDALVEPSDDDPTTLQ
                     GALLDAPDNSTVCMAAGTYRLNTEISISGEGVTLRGASRDTTILDFSTLDLGANGVKI
                     TGDNVTIKSLTVQETPGDGIRAEDVTNVTYDDVAVLWAAPGSVENGAYGFYPVGCTGV
                     TIRKSLVVGARDAGIYVGQSTDILVEDNEAHGNVAGIEIENSTGATVRRNHAHDNTAG
                     ILIFNLPGLPVKDGKQTLCSDNKVENNNLENFAKPGTIVSEVPPGLGFMILASDRNEI
                     RDNTITGHRSTGVIIAEYSSDLFEPFDDEEFDIYAQGNFVHHNMFADNGTDPAELILG
                     ISKNMKPAPDMMLDGCVDDSRDNSDNSLTNCFFENGTATYLNFDLCGGLAMQSSDLAP
                     VTCEHPPLMSPM"
BASE COUNT          497 a         1165 c          998 g          557 t
ORIGIN      
        1 gttgcgggaa gacacctgct tgcagggcca gcacggtatc cccgaggaga ttccgcagac
       61 ccgcgaggct ccgcccttgt tcgaggacct cgtcgaggac tgcctcgtca cgtccatcaa
      121 agtcctgcat cggtgcgtgg cgcaggaata cgctcgccga ctcgctggga acctcgcgcc
      181 cgttcaacag cgccaccggc tccaatgcca gcaccgcggc gacatcgtcg agctgtgtcg
      241 tcgtgatgcc cggcgctcct tgctcgagtg ccgcaagcag aggcaacggc acgccgctac
      301 gatccgccaa agtggcgagc gagagctgcc cacgctcacg tgcgagccga acgacttcgc
      361 cgatgcgctc aagcatccgg cctccttacc atggcgtcaa aagaacggtc cagccgacca
      421 cgactgctgc gcgccgcaag agtgcgcatc gcgcgcaacg cgtccgtgtc gggccccccg
      481 agtagcttca gcgacccgct gcggccgcac cagtgagtca ctgacacgcc acgtcgaccg
      541 gcatcgccgc gatccactca cggatcagtg ccagcccgcc ttggtcaatg acccggttcg
      601 gcagctcggg catcttgatc tccgggtcga gcgagttcat gcgaaaaacg acgatgctct
      661 cgtcgggctt gcccgggacg atgtcgtgcg cgtggccccc cgtgcccgca cccgcggccg
      721 ccggcacctt gcacacgccc accttggcga ggtccggctc gctcgccaag aacaccagcc
      781 cgctcttgcc cgccccgccg cccgcctgat ggcagtgcgc gcagttgccg tgcagataac
      841 cccgcgcgcg ctggtcgagc gtgccctcac cgccgtaggg atcgatgaac gccggaagcg
      901 tcgccaggtc tgccggcggc gccgtgaaca tgccctgctc cgccagccag gtcagctgcg
      961 cgacctgctt gccgtcgcgc tcgaccatgc ggttcatctg cggcgtaatc aacccgagcg
     1021 cgtgctcgac gtcgtcgcgc tcgtgacagc tgctgcactg gtcgtcgttg gggacgatat
     1081 attcctcggt cctcgccgcc ccgtcgacgt cggtgaactc gatgttgacg cgcttgccgg
     1141 tgcgcagcag cgtcgcctcc gtctgcgcgt cgttccacac gtaggtgaac gcgcgccagt
     1201 tgccggcctc acgcagcagc agccgcgtct cgatgatctt cgcgccggcc ttcgggtcgc
     1261 ggcggtcgtg gtcgaagaag aagttcttga ccaggatcgt gccctccgga aagccccact
     1321 cgccgtcgtc gctgcgcatc tcgatcttgc cgccctccgg caagacgatg aagcggtcct
     1381 tgccggcctg gtccgaccac agcggcgccg ccaccgtgta cggcaccacg ccggtcttgg
     1441 gccgcagctc gaccatcggc ccgacaaaga agtcgtactc ggccagcgac ttgaacggca
     1501 gcgccccctc ggcctgacag cgcgtcatgc cgtcgcacgg gtcgccctcg tcacagcccg
     1561 acaccagcat cgtcaccggc gccgccagca ccaccagcgc cagcaccacc cgcttgagca
     1621 tcatcattcg ctgcattcgc tgcatcatcc acatcgccgg cctcatccgc atccccgcgc
     1681 tcgcccgctc gccccggtca atcctcacat cggcgacatc agcggcggat gctcgcaggt
     1741 gaccggcgcg aggtcgctgc tctgcatggc gaggccgccg cacaggtcga agttgaggta
     1801 ggtcgcggtc ccgttctcga agaagcagtt cgtcaggctg ttgtcgctgt tgtcacgcga
     1861 gtcgtcgacg cagccgtcga gcatcatgtc cggcgccggc ttcatgttct tgctgatccc
     1921 caggatcagc tcggccgggt cggtgccgtt gtcggcgaac atgttgtggt gcacgaagtt
     1981 gccctgcgcg tagatgtcga actcctcgtc gtcgaagggc tcgaacaggt cgctcgagta
     2041 ctcggcgatg atgacgccgg tgctacggtg cccggtgatc gtgttgtcgc ggatctcgtt
     2101 gcggtccgag gcgaggatca tgaagccgag gcccggcggc acctcggaga cgatcgtgcc
     2161 cggcttggcg aagttctcga ggttgttgtt ctcgactttg ttgtcggaac acagcgtctg
     2221 cttgccatcc ttgaccggca gaccgggcaa gttgaagatc aggatccccg cggtgttgtc
     2281 gtgggcgtgg ttgcgccgga ccgtcgcgcc ggtcgagttc tcgatctcga tgcccgcgac
     2341 gttgccgtgg gcctcgttgt cctcgacgag gatgtcggtc gactgcccga cgtagatgcc
     2401 ggcgtcgcgc gccccgacga ccagcgactt gcggatcgtg acgcccgtgc agccgaccgg
     2461 ataaaacccg tacgccccgt tctcgaccga gcccggcgcg gcccacagca ccgcgacgtc
     2521 gtcgtaggtg acgttcgtga catcctcggc ccggatcccg tccccggggg tctcctgcac
     2581 cgtcaaggat ttgatcgtca cgttgtcgcc ggtgatcttg acgccgttgg ccccgaggtc
     2641 gagcgtcgag aagtcgagga tcgtcgtgtc gcgcgaggcc ccgcgcagcg tcacgccctc
     2701 gcccgagatc gagatctcgg tgttgaggcg gtacgtgccc gccgccatgc acaccgtgct
     2761 gttgtccggc gcgtccaaca acgcgccctg cagcgtcgtc gggtcgtcgt ccgatggctc
     2821 cacgagcgcg tcacagccct ccggcagcgc cgcgccgtcc gtgtccgtgt ccgtgtccgt
     2881 gcccgccgtg tcctccgtgc cgcccgagca ggcccccacg acacccagcg ccagcacacc
     2941 cgcgaatgac aacctgagac gtaccatgac cacgtttgca taacccactt cggccgtcct
     3001 ttgccagacc attcccggcg ctgctcgacc ccgctacaat ccccgcggag ccttcgcagt
     3061 gtcacaatac tacccgccgc gttcactaaa aaagaatctg agccgccgag gcttcctgcg
     3121 cgccaccctc gtgaccgcga gcgtcgcgag caccaccggg atgggcctgg gggccagcgg
     3181 ctgcggtaac gacgaggacg accggccgct gcaggac
//
LOCUS       DASWNJ010000497         2785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_609092, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000497 DASWNJ010000000
VERSION     DASWNJ010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609092"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VGB85_08445"
     CDS             complement(<1..814)
                     /locus_tag="VGB85_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134095.1"
                     /translation="MAGCGGAGSGTGPGTSSETATALMGTSTGEATPTTGVVEPGTTG
                     DTTGEPAACSDTTPADPPIAWDRGQPDIAGCTTRGLRDYRAIVHLHSHHSHDACDGEP
                     QPGGVPDEACLQDLRDALCITRIDIAFTTDHPVHAAEVPLEQLLLIRGDDEPVMGPGG
                     TPIASWMRCPDGHRVLMLPGIESGEMMPLGLEAHVTDAYGSAAPAAFQQIKDAGALAW
                     VAHTETRTLAELATLGLDGIEMYQLHANLDPDLREDYLGLDPGGFIAGVSPFF"
     gene            1135..2133
                     /locus_tag="VGB85_08450"
     CDS             1135..2133
                     /locus_tag="VGB85_08450"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEY0134096.1"
                     /translation="MKGPLELAFVGDIILGKYRLDAYAPLFAPGDDPFAAVADLLRAD
                     AAIANLETPLMHTRPERSPIYIGSRFGADKAAARALVNHFDALGVANNHALDLLTAGL
                     RETPEVLRELGLVPVGEARATGDPVQVATLERAGWRIALLAATTWTNRSFAPGDPALA
                     VYRTGELPRRLLPAVTAARVDHDLVIVLLHWGQEYSEGPDHAQRSVARKLIAGGADLV
                     VGHHPHVLQGLERHDTGLVAYSLGNFLFANTDERSRRSGVLRVRYAPGERCPERASFH
                     PVWLGEAPGFTPAPATGREAKQIRERVQAQSKKLRTALTLEGDALVLASWPCDPPP"
     gene            complement(2192..2662)
                     /locus_tag="VGB85_08455"
     CDS             complement(2192..2662)
                     /locus_tag="VGB85_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY0134097.1"
                     /translation="MSNYHSPGKRQRETNQARKKQEKAADRLDRRNRGPRDLPVVSAS
                     EMHGNLPSIEDAMRAIENPGSVERSASSIRVRLFVGSLSDSVTEADLRAAFEAFGPVS
                     DAVVMVERETGTPRGFGFVTMASRKDAPKAIEALNGSDLKGHSIVVNVATERGR"
BASE COUNT          358 a         1057 c          970 g          400 t
ORIGIN      
        1 agaagaacgg cgagaccccc gcgatgaagc cgccgggatc gagcccgagg taatcctcgc
       61 gcagatccgg atcgaggttg gcgtgcagct gatacatctc gatgccatcg aggcccagcg
      121 tcgccagctc ggcgagcgtg cgcgtctcgg tgtgcgcgac ccaggcgagc gcccccgcgt
      181 ctttgatctg ctggaaggcc gccggcgccg cgctcccgta ggcgtcggtc acgtgcgcct
      241 cgagcccgag cggcatcatc tcgccgctct cgatgcccgg cagcatcagc acgcggtggc
      301 cgtcggggca gcgcatccag ctggcgatcg gcgtgccgcc gggccccatg accggctcgt
      361 cgtcaccgcg gatcagcagg agttgttcga gcggcacctc ggccgcgtgc acggggtgat
      421 cggtggtgaa cgcgatgtcg atgcgggtga tgcacagcgc gtcgcgcagg tcctgcagac
      481 aggcctcgtc gggcacgccg cccggctgcg gctcgccgtc gcaggcgtcg tgcgagtgat
      541 gcgagtgcag gtgcacgatc gcccggtagt cgcgcagccc gcgggtggtg cagccggcga
      601 tgtcgggctg cccgcggtcc cacgcgatcg gtgggtcggc cggcgtcgtg tcgctgcagg
      661 ccgccggctc gccggtcgtg tcgcccgtgg tcccgggctc gacgaccccg gtggtcggcg
      721 tcgcctcgcc cgtactcgtg cccatcagcg ctgtcgcggt ctcgctgctc gtacctggcc
      781 cggtcccgct gcccgcgccg ccacagcctg ccatcaccac agcgatcgct gcctgtctgc
      841 ccgtccagcg catcgaggca cgttatcacg ctcgcccggc gccgccgtcc gtgagaccct
      901 gcgcccacga tgctccgccg ggccttgctc gtcgccccgc tgctcggctg ttcgagcccc
      961 gtccacaccc aaccccgggt cagctcacac gccacgccgg cgcccgcccc ggaacctgcc
     1021 ccggaacctg gcccttcgaa cctgtccccc gcgccagctc cggcgcccgc cccggcgccc
     1081 gcccccgaac ctggcccttc ggacctgtcc cccgcgccag ctccggcgcc cgccgtgaag
     1141 ggcccgctcg agctggcctt cgtcggcgac atcatcctcg gcaagtaccg cctcgacgcc
     1201 tacgccccgc tgttcgcgcc cggggacgac cccttcgccg cggtcgcgga cctgctgcgc
     1261 gccgacgcag cgatcgccaa cctcgagacc ccgctgatgc acacccgccc cgagcgcagc
     1321 ccgatctaca tcggctcgcg cttcggcgcc gacaaggcgg ccgcgagggc tcttgtaaac
     1381 cacttcgacg cgctcggggt cgccaacaac cacgcgctcg acctgctgac cgccggcctg
     1441 cgcgagaccc cggaggtgct gcgcgagctc ggcctggtgc ccgtcggcga ggcgcgtgcg
     1501 accggcgacc ccgtgcaggt cgccacgctc gagcgcgccg gctggcggat cgcgctgctc
     1561 gccgcgacca cgtggaccaa ccggtcgttc gcgcccggcg accccgcgct ggccgtgtac
     1621 cgcaccggcg agctgccgcg gcgactgctg cccgccgtca ccgcggcgcg cgtcgaccac
     1681 gacctcgtga tcgtgctgct gcactggggt caggagtaca gcgagggccc cgaccacgcg
     1741 cagcgcagcg tcgcccgcaa gctgatcgcc ggcggcgcgg acctcgtcgt cggccatcac
     1801 ccgcacgtgc tgcaagggct cgagcgccac gacaccggcc tcgtcgccta ctcgctcggc
     1861 aacttcctgt tcgccaacac cgacgagcgt tcgcggcgca gcggcgtgct gcgcgtccgc
     1921 tacgcgcccg gcgagcgctg ccccgagcgc gccagcttcc acccggtctg gctcggcgag
     1981 gccccgggct tcacgcccgc gcccgccacc ggccgcgagg ccaagcagat ccgcgagcgc
     2041 gtgcaggcgc agagcaagaa gctgcggacc gccctgacgc tcgagggcga cgcgctggtg
     2101 ctcgcgagct ggccctgcga tccgccgcca tgaaacgtgg cggggatgca gggccagggc
     2161 acacgcccgg aggcgcgttc gggcgcgtcg gctagcggcc gcgctcggtg gcgacgttga
     2221 cgacgatcga gtggcccttg aggtccgagc cgttgagcgc ctcgatcgcc ttgggggcgt
     2281 ccttgcggct cgccatggtc acgaagccga agccgcgggg ggtgccggtc tcgcgctcga
     2341 ccatgacgac ggcgtccgac acggggccga aggcctcgaa ggcggcgcgg aggtcggcct
     2401 cggtgacgct gtcgctgagg ctgccgacga acagccgcac gcggatgctc gacgcgctgc
     2461 gctcgacgga gccggggttc tcgatcgcac gcatcgcgtc ctcgatggag ggcaggttgc
     2521 cgtgcatctc ggaggcgctg acgacgggga ggtctcgcgg accacggtta cgccggtcga
     2581 ggcggtcggc cgccttttcc tgcttcttgc gcgcctggtt ggtctcacgc tggcgtttgc
     2641 cgggcgaatg ataattgctc atgtctgacc tcctcgaagg gggtgaaagc cggctcggat
     2701 aggaaacgac gcagaatttt gcgacgcgcc tcgattgccg ggcgagaact gggagattcg
     2761 cgggggcaga gtctactgca agtac
//
LOCUS       DASWNJ010000498         3697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_292047, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000498 DASWNJ010000000
VERSION     DASWNJ010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292047"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1571)
                     /locus_tag="VGB85_08460"
     CDS             complement(6..1571)
                     /locus_tag="VGB85_08460"
                     /inference="COORDINATES: protein motif:HMM:NF045061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134098.1"
                     /translation="MSRLALPVLLIASACSSNPGDGGDASSDAGTTSTGAPATPTTGE
                     DSDTGATPVTSGSGDASSTGEPALPGPWDEGWPIPEYPQIPGDPVAGYEALLTRGYVS
                     CGVPYSFWGLAKGSLGDFTRGDPLPGRSGKNAEVPYNWTVHTPASGVEIVSLNCLECH
                     AGNFNNQLVVGLGVADMDFTDVPMGALVEALPIPDIFPGAVGELAKFAGRYKAIGPNI
                     KMLTVGTNPADMIAAQLAAHRDPVTLEWSDEPSYDAPAVMAPIDTPPWWRAHKKHGLF
                     YNGMARTDHRGTMMFASSLCADSVAEASEILSYFNDINAYIRSLRPPKYPFAIDAALA
                     EQGAGVFAANCAGCHGTYAEDEAKETYPNLLFPLDIIGTDPTLAEAGAAGGSTSKSVD
                     WFNSSYYGQLLQLVTDDPFPGYVAPPLDGVWATAPYLHNGSVPTLDVLLNSQLRPTYW
                     KRIDLDSGHYDQAKLGWPILVLPYGQDGAPDDERKHVYDTTQPGHANTGHPFGDHLTD
                     AERAAVLEYIKTI"
     gene            complement(1617..2759)
                     /locus_tag="VGB85_08465"
     CDS             complement(1617..2759)
                     /locus_tag="VGB85_08465"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0134099.1"
                     /translation="MSAGTPPVLRADLGQLDRLRTPVWIVDLERGEKWWCNRSGLALW
                     NAATLAEWLGRNATNTVSEATLTRLHNMRRRLERGEVTTERWTFYPDGADPVVAECQV
                     SGIVITDAPDEPGRTAMMIEARPLRADEVDPSERRGYEALRYLGELVSYYADTGEALM
                     RNPAALRALGDTGAGDQLAAGFVDPAQASTLRACIAGGAVYRGDALMRTATGERWFDT
                     EARASLDPITGKYGVLVTQRDISERRQHLEELERRGQRLAEQAETLRHLAAPVMRVGP
                     GVLALPLIGALDRPRVDVALAALLARTGSERVSRVVLDLTGATAVDLAAADGVLRILR
                     VLQLQGVTPVLSGIQPGLARAIVAGGLDLGRVRVYQTMAEALGAPL"
     gene            complement(2808..3458)
                     /locus_tag="VGB85_08470"
     CDS             complement(2808..3458)
                     /locus_tag="VGB85_08470"
                     /inference="COORDINATES: protein motif:HMM:NF015423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HEY0134100.1"
                     /translation="MPTHDFVLPLHVDLGATFLFALSGALAAARRSYDPVGLFVLALV
                     TGVGGGLLRDGLFLQQGPPAFVQEPMYLLVVACASVLGYFFAAALARIGAVLFTADAL
                     GLGAYAVVGVQKSLALGLGVAAAILIGVVNAVGGGLLRDVLVREEPFLFKPGDFYALV
                     AAAGAGLFCLLTMVAGWSSTQAAAAAIPVTFALRLLSHRMGWTTRPARIDPPSGAR"
     gene            complement(3464..>3697)
                     /locus_tag="VGB85_08475"
     CDS             complement(3464..>3697)
                     /locus_tag="VGB85_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY0134101.1"
                     /translation="IRVRVGIDTGPVLVFDLASGGKDIAGAPVNIASKMAQDRGEWGH
                     IYLSERLRGLAQARGFTPLHYVVSGVELRVFAG"
BASE COUNT          529 a         1240 c         1417 g          511 t
ORIGIN      
        1 tcgcgtcaga tcgtcttgat gtactcgagc accgccgcgc gctcggcgtc tgtcaggtga
       61 tcgccgaacg ggtggccggt gttggcgtgg cccggctggg tcgtatcata gacgtgcttg
      121 cgctcgtcgt ccggcgcgcc atcctggccg tagggcagca cgaggatcgg ccagccgagt
      181 tttgcctgat cgtagtgacc gctgtcgagg tcgatccgct tccagtaggt cgggcgcagc
      241 tgtgaattca agaggacatc gagcgtgggc accgagccgt tatgaagata tggcgcggtc
      301 gcccacacgc catcgagcgg cggcgcgacg tagcccggga acgggtcgtc ggtgaccagc
      361 tgcagcagct ggccgtaata ggagctgttg aaccaatcca ccgacttgct ggtgctgccg
      421 cccgcggcgc cggcctcggc gagcgtcggg tcggtgccga tgatgtcgag cgggaacagc
      481 aggttggggt aggtctcctt ggcctcgtcc tcggcgtagg tgccgtggca cccggcgcag
      541 ttggccgcga acaccccggc gccctgctcg gcgagcgcgg cgtcgatcgc aaacgggtac
      601 ttcggcgggc gcagggagcg gatgtacgcg ttgatgtcgt tgaagtacga caggatctcg
      661 ctcgcctcgg cgacgctgtc ggcgcacagc gaggacgcga acatcatggt gccgcggtgg
      721 tcggtgcgcg ccatgccatt ataaaatagg ccgtgcttct tgtgcgcgcg ccaccacggc
      781 ggcgtgtcga tcggggccat gacggccggc gcgtcgtacg acggttcgtc gctccactcg
      841 agcgtcaccg ggtcgcggtg ggcggcgagc tgcgcggcga tcatgtcggc cgggttggtg
      901 ccgacggtca gcatcttgat gttcgggccg atggccttgt agcgcccggc gaacttggcg
      961 agctcaccga ctgcccccgg gaagatgtcg gggatcggga gggcctcgac gagcgcgccc
     1021 atcggcacgt cggtgaagtc catgtcggcg acgccgaggc cgacgaccag ctgattatta
     1081 aaattgccgg cgtggcactc gaggcagttg aggctgacga tctccacgcc gctcgcgggc
     1141 gtgtgcacgg tccagttgta ggggacctcc gcgttcttgc cgctgcggcc gggcagcggg
     1201 tcgccgcgcg tgaagtcgcc gagcgagccc ttggcgagcc cccagaacga gtacgggacg
     1261 ccgcagctga cgtagccccg ggtcagcagc gcctcatatc cggccaccgg gtcgccgggg
     1321 atctgcgggt attcggggat cggccagccc tcgtcccacg ggcccggcag cgcgggctcg
     1381 ccggtgctgc tggcgtcgcc ggacccgctc gtgaccggtg tcgcgccggt gtcgctatcc
     1441 tctcccgtgg tcggcgtggc aggggcgcct gtcgaggtcg tgccggcgtc gctgctggca
     1501 tcgccgccgt caccggggtt gctgctgcag gcggaggcga tgagaaggac cgggagggcg
     1561 aggcgcgaca tcccgccacc atatcagggg cgagagcgcg cgtgaagccg cggggatcac
     1621 agcggagcgc cgagggcctc ggccatcgtc tggtagacgc gcacgcggcc gaggtcgagg
     1681 ccgccggcga cgatcgcccg ggcgagccct ggctggatcc cgctgagcac cggggtgacg
     1741 ccctggagct gcaggacgcg gaggatccgc aggaccccgt ccgcggccgc gaggtcgacc
     1801 gccgtcgcgc cggtcaggtc gagcacgacg cgggagaccc gctcggagcc ggtgcgggcg
     1861 agcagcgccg cgagggcgac gtcgacgcgg gggcggtcga gcgcgccgat caacggcaac
     1921 gcgagcacgc cggggccgac gcgcatcacc ggcgccgcga ggtggcgcag ggtctcggcc
     1981 tgctcggcga gccgctggcc gcgacgctcg agctcctcga ggtgctggcg gcgctcgctg
     2041 atgtcgcgct gggtgacgag cacgccgtac ttgccggtga tcgggtcgag gctcgcgcgc
     2101 gcctcggtgt cgaaccatcg ctcgccggtg gcggtgcgca tcagcgcgtc gccgcgatac
     2161 acggcgccgc cggcgatgca ggcgcgcagc gtgctggcct gggccgggtc gacgaagccg
     2221 gcggcgagct ggtcgcccgc gccggtgtcc ccgagggcgc gcagggccgc ggggttgcgc
     2281 atcagcgcct cgccggtatc cgcgtagtac gacaccagct cgccgaggta gcgcagcgcc
     2341 tcgtagccgc ggcgctccga ggggtcgacc tcgtcggcgc gcagcggccg ggcctcgatc
     2401 atcatggcgg tgcgtccggg ctcgtcgggg gcgtcggtga tgacgatgcc cgagacctgg
     2461 cactcggcga ccaccgggtc ggcgccgtcg ggataaaatg tccagcgctc ggtggtgacc
     2521 tcaccgcgct cgaggcggcg gcgcatgttg tggagccgcg tcagggtcgc ctcggacacc
     2581 gtgttggtcg cgttgcggcc gagccactcc gcgagggtcg cggcgttcca cagcgcgagc
     2641 ccgctgcggt tgcaccacca cttctcgccg cgctcgaggt cgacgatcca cacgggcgtg
     2701 cgcaggcgat cgagctggcc gaggtcggcg cgcaggacgg gtggggttcc ggcggacatg
     2761 cgggtcgatg gagcgtcgcc cgttcgcgcc ggggatgcca aggggtgtta gcgcgccccg
     2821 gacggcgggt cgatgcgggc cgggcgcgtc gtccagccca tgcgatgcga cagcaggcgc
     2881 agcgcgaagg tgacggggat cgccgccgcg gcggcctgcg tcgagctcca gccggcgacc
     2941 atggtgagca ggcagaacag ccccgcgccg gccgccgcga cgagcgcgta gaagtcgccc
     3001 ggcttgaaca ggaacggctc ctcgcggacc aggacgtcgc gcagcaggcc gccgccgacg
     3061 gcgttgacga ccccgatcag gatcgccgcg gcgacgccga ggccgagcgc gagcgacttc
     3121 tgcacgccga ccaccgcgta ggcgccgagg ccgagcgcgt cagcagtgaa tagcaccgcg
     3181 ccgatgcggg ccagcgccgc cgcgaagaag tagccgagca cgctcgcgca cgcgaccacc
     3241 agcaggtaca tcggctcctg cacgaacgcg ggcgggccct gctgcaaaaa caggccgtcg
     3301 cgcaacagcc ctccgccgac gccggtcacc agcgcgagca cgaacaggcc gaccgggtcg
     3361 tagctgcggc gcgcggcggc cagcgccccg ctgagcgcga acaaaaaggt cgcgccgagg
     3421 tcgacgtgga ggggcagcac gaagtcgtgg gtcggcatga cggtcaacct gcgaacaccc
     3481 ggagctcgac gcccgacacc acataatgca gcggggtgaa cccgcgcgcc tgcgcgaggc
     3541 ctcgcaggcg ctcgctgagg tagatatggc cccactcgcc gcggtcctgg gccatcttcg
     3601 aggcgatgtt gacgggcgcg ccggcgatgt ccttgccgcc gctggcgagg tcgaacacga
     3661 ggaccgggcc ggtgtcgatg ccgacgcgca cgcggat
//
LOCUS       DASWNJ010000499         2799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_503308, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000499 DASWNJ010000000
VERSION     DASWNJ010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503308"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VGB85_08480"
     CDS             <1..271
                     /locus_tag="VGB85_08480"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEY0134102.1"
                     /translation="RRLGPALGLNPAPRSALRRNPMNRLSSPIEAISAQYEAVVIGTG
                     YGGALMASRLARAGLKVCVLERGRERHTSTRTSTCSSAAASAAPR"
     gene            235..1740
                     /locus_tag="VGB85_08485"
     CDS             235..1740
                     /locus_tag="VGB85_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0134103.1"
                     /translation="MLVGCGLGGTSLINANVSIRAEPRVFADPRWPADLRAENMKHLD
                     AGYALAEHMLSPRPYPESYPPLPKLVALQRSAEAMRQPFRRTNINVTFEDGINSAGVA
                     QKACINCGDCCSGCNYSAKNTVLMNYLPDAKRCGAEIFVEVGVRHVERRDDGLWNVHY
                     QVLDTGREKFDAPTLFVTAKIVVLSAGTLGSTEILLRSKQRGLSLSDRVGQGFSGNGD
                     MLGFGYNCEPVLEGIGHGHHPPGSMPPVGPCITGIIDMRDAPGLADDIIIEEGSIPGA
                     IAPLLGLAFQLAADYSGTNTAPETAFAQRLRELDTLVRGPYHGAARHTQTYLVMGHEA
                     NDGVMKLADDALRIDWPGVGDQPIFAKMNAHLRDATSALSGILVRDPIWNPKLGERLH
                     ELFGEGTMQAFEGLATMVRKGHVVAADGAAVYLPHLDRMALPIRFIHGAENQCYLPAS
                     TEETVAALSAVNDPALYSREVIPGYGHIDCIFGKNAAVDVFPFIVEHLDAT"
     gene            complement(1972..2271)
                     /locus_tag="VGB85_08490"
     CDS             complement(1972..2271)
                     /locus_tag="VGB85_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEY0134104.1"
                     /translation="MPLHRAGIQAVVASRFPLSVVGSIALTAGLYRHLLVDLGSVEEG
                     VAAARMVLARAPEQLDWASVQLYGRPADGGTRPWVIRPYRGLLAYQREHQRLFSR"
BASE COUNT          467 a         1033 c          897 g          402 t
ORIGIN      
        1 ccgccgcctg gggccggcgc ttggcctgaa tcccgcaccg cgatccgcac tccgaaggaa
       61 cccgatgaac cgcctgtcct cccccatcga agcgatctcc gcgcagtacg aagcggtcgt
      121 catcggcacc ggctacggag gcgcgctcat ggcgtcccgc ctcgcccgcg ccggtctcaa
      181 ggtctgcgtg ctcgagcgcg gccgggagcg ccacacctca acaaggacat caacgtgctc
      241 gtcggctgcg gcctcggcgg cacctcgctg atcaacgcca acgtctcgat ccgggccgag
      301 ccgcgcgtgt tcgccgaccc gcgctggccc gccgacctgc gcgccgagaa catgaagcac
      361 ctcgacgccg gctacgcgct cgccgagcac atgctctcgc ccaggccgta cccggagtcc
      421 tatccgccgc tgcccaagct cgtcgccctg cagcggtccg ccgaggcgat gcgccagcca
      481 tttcgccgca ccaacatcaa cgtcaccttc gaggacggca tcaacagcgc cggcgtcgcc
      541 cagaaggcct gcatcaactg tggcgactgc tgctcgggct gcaactacag cgccaagaac
      601 acggtgctga tgaactacct gcccgacgcg aagcgctgcg gggccgagat cttcgtcgag
      661 gtcggggtcc gccacgtcga gcgccgcgac gacggcctgt ggaacgtgca ctaccaggtc
      721 ctcgacaccg gccgcgagaa gttcgacgcg ccgacgctgt tcgtgaccgc caagatcgtg
      781 gtcctctcgg cggggacgct cggctcgacg gagatcctgc tgcgctcgaa gcagcgcggc
      841 ctgtcgctgt ccgaccgcgt cggccagggc tttagcggca acggcgacat gctcgggttc
      901 ggctacaact gcgagccggt gctcgagggc atcggtcacg gtcaccaccc gccgggctcg
      961 atgccgccgg tcggcccatg catcaccggc atcatcgaca tgcgcgacgc ccccggcctc
     1021 gccgacgaca tcatcatcga ggagggatcg atccccggcg cgatcgcccc gctgctcggg
     1081 ctcgcgttcc agctcgccgc cgactacagc ggcacgaaca ccgcgccgga gacggccttc
     1141 gcccagcgcc tgcgcgagct cgacaccctc gtccgcggcc cgtaccacgg cgcggcgcgg
     1201 cacacccaga catatctggt gatgggccac gaggccaacg acggggtgat gaagctggcc
     1261 gacgacgcgc tgcgcatcga ctggcccggc gtcggcgacc agccgatctt cgccaagatg
     1321 aacgcgcacc tgcgcgacgc gacgagcgcc ctctcgggga tcctcgtgcg cgacccgatc
     1381 tggaacccga agctcggcga gcggctccac gaactgttcg gcgaggggac gatgcaagcg
     1441 ttcgagggcc tggcgacgat ggtccgcaag ggccacgtcg tcgccgcgga cggggccgcc
     1501 gtctacctgc cgcacctcga tcgcatggcc ctgccgatcc gctttatcca cggcgcggag
     1561 aatcagtgct acctgcccgc gagcaccgag gagaccgtcg cggcgctgtc ggcggtcaac
     1621 gacccggccc tgtactcccg cgaggtcatc cccggctacg gccacatcga ctgcatcttc
     1681 ggcaagaacg ccgcggtcga cgtgttcccg ttcatcgtcg agcacctcga cgcgacctga
     1741 gggtcccgag cgatgcgctt tgacggccgc ggccgtcaaa gcggacgact agcctcctgg
     1801 tctttacctg tcagatccgc cgactcgacc ggcgtcacca aggtggctcc cgccagggcg
     1861 agatcgaatt ccagcgtccg ttgaaccacc gctcggggat ccttgcggac tgaatacttg
     1921 aactgggaca cgcgcgcaaa cccaccccgg tgcctgccca agacctctgg atcagcgcga
     1981 gaacaggcgc tggtgctcgc gctgatacgc gagcaggccg cgatacggtc ggatcaccca
     2041 cggccgcgtg ccgccgtccg cgggtcggcc gtacagctgc acgctcgccc agtcgagctg
     2101 ctccggggcg cgcgcgagca ccatccgcgc cgccgccaca ccctcctcga ccgatccgag
     2161 gtcgacgagc aggtgacgat agagcccggc ggtcaatgcg atcgatccga ccaccgacag
     2221 cggaaatctc gacgcgacca ccgcctggat ccccgcccgg tgcagcggca tcctcggcac
     2281 tggccacatc ggcaagactc tggtccgcaa gctgcgtgca tctggacatt acgtgaaggc
     2341 ggccaactcg cgcggcccct cgaccatcgc ggccgaattg cggcacgcct ccaaggatca
     2401 ctatctcagc accgaaacga tgactggccc ggagctcgcc acgatgctga gcgacgtcct
     2461 cgggcggacg atcagatgcg acgagccggg tgacactcgc aacggttacc tcgacgagga
     2521 cacccgccga gagcgtcgag cgacggtctc cgctgaagga gctcgtcgac gctgcgcgcg
     2581 gttgctccgt ggcgctttga gatcggcgag ttccgcggca atatgtcgga cgtgggtctc
     2641 cactccggca ccgaagacat cgatagagcg atcgtgccgc ggacccggtc gcggcgtatc
     2701 ctcggggtga cggccgccct cagcgctgtc ttgctcgcgt gtcggcgtcc ggctccgtcg
     2761 gcagaggagc gaccagacga ggctgatcgt gcggaggcg
//
LOCUS       DASWNJ010000500         3986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_9520, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000500 DASWNJ010000000
VERSION     DASWNJ010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9520"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            466..1770
                     /locus_tag="VGB85_08495"
     CDS             466..1770
                     /locus_tag="VGB85_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134105.1"
                     /translation="MSEPPVIETIADTPPFDPPATTPPPAPAPQDVAAVVVTPAVLEV
                     RVLAYFTGERVPMLHRLADRRLFVSEGPRIAEVPARASATAVKVRIYGGGLGWIGLPR
                     VTPRSYDSPFSGEYLESFGGRWPAALATIAFRAPRTGTVRGVFRRAGSGWRRLADESN
                     TGGTYAAIAPWTEGRVLALRVEDMFSGTGKDFAPGFVVFGAKTPAPALPADFRPYDMD
                     TTAAGDVFVVGARGEARTLWVLRWGPAGGEPLSSDRLPDFRDMPAHESQWFIKAASAT
                     QVHVGGGYAVDPAQATWTPYLAALAGGVWTELRTPLVGRVGSMVHAPDGALWVSASAE
                     YANNYHVPGRGDRVYEGGLWRHTQAEGWSSVTLSTHDYHTKREQPLDLGRYMVRAVGV
                     HGDRLCLLASPNAGRVDYAVHRGSMLACAVDAPSAAPIPAPS"
     gene            complement(1791..2531)
                     /locus_tag="VGB85_08500"
     CDS             complement(1791..2531)
                     /locus_tag="VGB85_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134106.1"
                     /translation="MHYLIRRVATLSLLAALPGACTEKGEETTAASATETSGDTSGEP
                     TGGSSDDSTGATSDDSTGMTEPTTGEPSPVCADYTEWVFECGQGDPLSEEEVLGECER
                     EREVMAAVYGPSCAAKYDAFTECISDNACDAFDACDAEAEASDLCLPEAGATCMKFAA
                     TVAACEPGEGEAEIGGSCQLDINAGGHDNAECGAAIEAMYACYSDLTCEQIAGDGAAC
                     SQEVAMLEALCPGEGGLMQSRHLDAHRR"
     gene            complement(2887..3309)
                     /locus_tag="VGB85_08505"
     CDS             complement(2887..3309)
                     /locus_tag="VGB85_08505"
                     /inference="COORDINATES: protein motif:HMM:NF023390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HEY0134107.1"
                     /translation="MERPTASGRHEFWYEGELFGVRFRGSITRRDVEVLRQAVEAVAE
                     EHPRSYLLIDLHGSTGIDPEARKYLVEWSKVPEQGLVGTVVYGTGFVMRSLVTLSINA
                     IRLLGHLKGEFHFFKDEAEARRWADGHRARTGGAQRDA"
     gene            complement(3404..>3986)
                     /locus_tag="VGB85_08510"
     CDS             complement(3404..>3986)
                     /locus_tag="VGB85_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134108.1"
                     /translation="LLAAPASGKHVAVTSWQCPDDPRNFLLTSFLGREPPQILALHRR
                     VLASARCQPVPGGHPAPVFPRFAPPGAQRIDNPAALVFQGAQGEVYTMSAGLPGRELI
                     DKVALNPNFATTAVTVEFPVRDVVLPAAPTVQGDRRTWFGRARGDAGDAVRVGITVWH
                     CPGPDLSFVGMFAGPEAVVEAQALQILESAQCP"
BASE COUNT          559 a         1416 c         1405 g          606 t
ORIGIN      
        1 cgatactgcg agcacttttt gaatgcgcaa tcaagaattt tcgtgtctgc gtgcatccta
       61 cgtcagattc caccgatagg gcacaaactg aaggcgacac atcgacggta actcgcaatg
      121 gcgtgatagc agtggcaggt atttcctggt accgcacacg gcgctcatcc gagacccgtc
      181 agacgcccgt tcgccgcccc ggccgccaag tctgccgagg cagccggcgc aaaggtccgc
      241 gcctgtgccg tgaacaccca catcggcgac cgcgggtcgc aagcagagaa tgttcgctcg
      301 ctcgccggcc gagagtcgac gataggcccg gcgtccgcga catgccggag atgtatggag
      361 cgatgcccgg gcgtgctacc gtgacccatg gcgagcgtgc gtgcgagcgg tatggtgcga
      421 ggccgcgcat gcttgatcgg cgcgctggcg tgcgcctgcc agccggtgag tgagccgccc
      481 gtgatcgaga cgatcgccga cacgccgccg ttcgatcccc cggcgaccac ccctccgcct
      541 gctcctgcgc cgcaggatgt cgcggccgtg gtcgtgacgc cggcggtgct cgaggtccgc
      601 gtgctcgcgt atttcaccgg cgagcgcgtt cccatgctgc accgtctcgc cgatcggcgg
      661 ctgttcgtca gcgaggggcc gaggatcgcg gaggtcccgg cgcgggccag cgcgaccgcg
      721 gtgaaggtgc gcatttacgg gggcgggctc ggctggatcg gcctcccgcg cgtgacgccg
      781 cggtcgtacg acagcccgtt ctccggcgag tacctcgaga gtttcggcgg ccgctggccc
      841 gcggcgctgg cgacgatcgc gtttcgggcc ccgcgcacgg gcaccgtccg cggcgtgttt
      901 cgtcgcgccg gctccggctg gcgccggctc gccgacgagt ccaacaccgg cggaacgtac
      961 gcagcgatcg cgccgtggac cgaggggcgc gtgctcgcgc tgcgcgtcga ggacatgttc
     1021 tccgggacag gtaaggactt cgcgcccggg ttcgtggtgt tcggcgcgaa gacgccggcg
     1081 ccggcgctgc cggccgactt ccggccgtat gacatggaca ccaccgcggc gggcgacgtg
     1141 ttcgtggtcg gcgcccgtgg cgaggcgcgt acgctgtggg tgctgcgctg gggacccgcg
     1201 ggcggcgagc cgctgagcag cgatcgcctg ccggacttcc gcgatatgcc cgcccacgag
     1261 tcccagtggt tcatcaaggc cgcgagcgcc acgcaggtcc acgtcggcgg cggttatgcc
     1321 gtcgaccccg cgcaggcgac gtggacccca tacctggccg cgctcgcggg cggtgtctgg
     1381 accgagctgc gcacgccgct ggtcgggcgc gtcggctcga tggtccacgc gcccgacggc
     1441 gccctgtggg tctcggcgtc cgcggaatat gccaacaact atcacgtccc gggtcgcggc
     1501 gaccgggtgt acgagggtgg tttgtggcgc catacgcagg ccgaaggctg gagcagcgtc
     1561 acgttgagca cgcacgacta tcacacgaag cgcgagcagc cgctcgacct cgggcgttac
     1621 atggtacgcg ccgtgggggt ccacggcgat cggctgtgcc tgctagcgag cccgaacgcg
     1681 ggtcgcgtcg attacgcggt tcaccgcgga tccatgctgg catgtgccgt ggacgccccg
     1741 tccgcggcgc cgatcccggc gccgtcctga cggccctcgt gccggctcgc tcagcggcgg
     1801 tgcgcgtcga ggtgcctcga ctgcatcagc ccgccctcgc cggggcacag cgcctcgagc
     1861 atcgccacct cttgcgagca agcggcgcca tcccccgcga tctgctcgca ggtgagatcg
     1921 ctgtaacaag cgtacatcgc ctcgatggcc gcgccgcact cggcgttatc gtggccgccg
     1981 gcgttgatat cgagctggca gctgcccccg atctcggcct cgccctcgcc cggctcgcag
     2041 gcggccacgg tcgcggcgaa cttcatgcag gtggcgccgg cctccggcag gcacaggtcg
     2101 gaggcctcgg cctccgcgtc gcaggcgtcg aaggcgtcgc acgcgttgtc gctgatgcac
     2161 tcggtgaagg cgtcgtactt ggccgcgcag ctgggcccgt agaccgcggc catcacctcg
     2221 cgctcgcgct cgcactcgcc gagcacctcc tcctcgctga gcgggtcgcc ctgcccgcac
     2281 tcgaacaccc actccgtgta gtcggcgcac accgggctcg gctcgccggt ggtcggctcg
     2341 gtcatgccgg tcgaatcgtc actcgtcgcg ccggtcgaat cgtcgctcga tccgccggtc
     2401 ggctcgccgg acgtatcgcc ggacgtctcc gtggccgaag cggcggtcgt ctcctcgccc
     2461 ttctcggtgc aggcgccggg cagcgcggcg aggagcgaga gcgtcgcgac acgtcgaatc
     2521 aggtaatgca tggccgacat cctgacagcg ccggccgagc cgcgccagcg atcgtcgtat
     2581 gatattttcg cgcgcatgta cggcgcggct gtcctggcgg caaaccgcgc ctgtggtcca
     2641 gagattcacc gccggtctcc gcatagtgtc cgtacgattc accgtgatgt ccgcgtaatc
     2701 atgggcggtc acgatcggct gtacgaacga ctgcgacgat aaggtcgctg tcgtgctgcc
     2761 ggccccactc cgccatcatc cggcgcgcgt cggcgtcgat gcccgcgagc tcggcggcat
     2821 cggcgaccag gaagcagcgc ccgcgctcga tcgtccggct cggcccgcga ggaccgtccg
     2881 gtgcggtcat gcgtcgcgct gcgcaccgcc ggtccgggcc cggtgcccgt cggcccagcg
     2941 gcgggcctcg gcctcgtcct tgaagaagtg gaactcgccc ttcaggtggc cgagcagcct
     3001 gatcgcgttg atggagagcg tgaccagcga gcgcatcacg aagccggtcc cgtacaccac
     3061 ggtgccgacg aggccctgct ccggcacctt gctccactcg acgaggtact tgcgggcctc
     3121 ggggtcgatg ccggtgctgc cgtgcaggtc gatcagcaag tacgatcgcg ggtgctcctc
     3181 ggcgacggcc tcgaccgcct gacgcagcac ctcgacgtcc ctgcgcgtga tcgacccgcg
     3241 gaagcgaacc ccgaacagct cgccctcgta ccagaactcg tgtcggccgg acgccgtcgg
     3301 tcgctccacg tcgggcactg taacacaggc gtgcgccgtc acggcgcgcg gacccggctc
     3361 gccgacgcgg tcctgccggc ccgcctctgc ggaggacgac ggcttatggg cactgcgcag
     3421 attcgaggat ctgcagggcc tgcgcctcga cgaccgcctc ggggccggcg aacataccga
     3481 caaaggagag gtcgggcccc gggcaatgcc atacggtgat cccgacgcgc acggcgtcgc
     3541 ccgcgtcgcc gcgcgcgcga ccaaaccatg tccggcggtc gccctgcacc gtcggcgcgg
     3601 ccggcagaac gacgtcacgg acggggaact cgacggtgac cgcggtggtc gcaaagttcg
     3661 gattgagggc gaccttgtcg atcagctcgc gccccggcag gccggcggac atcgtgtaga
     3721 cctcgccctg ggcgccctgg aacaccagcg ccgcgggatt atcgatgcgc tgcgcgccgg
     3781 gtggcgcgaa gcggggaaac accggcgccg ggtgaccccc ggggaccggc tggcagcgcg
     3841 cgctggccag cacgcggcga tgcagcgcca ggatctgcgg cggttcgcgg ccgaggaagc
     3901 tcgtcagcag gaagttgcgc gggtcgtcgg ggcactgcca ggaagtcacc gcgacgtgct
     3961 ttcccgacgc cggggccgcc agcaac
//
LOCUS       DASWNJ010000501         1747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_503325, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000501 DASWNJ010000000
VERSION     DASWNJ010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503325"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1584)
                     /gene="rtcR"
                     /locus_tag="VGB85_08515"
     CDS             complement(<1..1584)
                     /gene="rtcR"
                     /locus_tag="VGB85_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA repair transcriptional activator RtcR"
                     /protein_id="HEY0134109.1"
                     /translation="MRRATVVLGLLGTSLDASPKARWERWRPTVDLCRREDFLVDRLE
                     LLHSPQHQELAGTVATDIASVSPETRVCTHALSFADPWDFESVFATLLDFAHGYPFRP
                     DDEDYLVHITTGTHVQQICLFLLTEARYLPGKLLQTSPPTPAHSGEPGDLRVIDLDLA
                     SYRQIAERVQAARSEGLSFLKAGIATRNPAFNALVDQIERVATATRSPILLTGPTGAG
                     KTRLARKIFELKQTRHQIAGRFFELNCATLRGDAAMSAMFGHTRGAFTGAVRDRPGVL
                     KSADGGVLFLDEIGELGLDEQAMLLRALEDHTFLPVGSDREVHSDFQLIAGTNRDLRA
                     CVREGKFREDLLARIDLWTFRMPSLRERPEDIEPNLEYELERYAQRHGRRVTFGRAAR
                     AHFLKFAAGPDARWPGNFRDFAAALERMATLAPGAKIGAAEVEAETRRLRDAWSEGQV
                     DGDAVLLRRYLNNEQIEALDRFDRVQLADVLRVCQESRSLSDAGRVLFAASRQRRRSQ
                     NDADRLRKYLQKFDIEWSEL"
BASE COUNT          299 a          580 c          582 g          286 t
ORIGIN      
        1 cagctcggac cactcgatgt cgaacttttg caaatacttc cttaaacggt cggcgtcgtt
       61 ttgcgagcgc cggcgctggc gcgacgcggc gaacaacacc cgacccgcgt ccgacaggct
      121 acgcgactcc tgacacacgc gcaatacatc cgcgagctgc acgcggtcga atctatcgag
      181 cgcttcgatc tgctcattat taagatatcg gcgcagcagc accgcgtcgc cgtcgacctg
      241 tccctccgac caggcgtcac gcaggcgcct ggtctccgcc tcgacctcgg cagcgccaat
      301 tttagccccc ggcgccagcg tggccatgcg ctcgagcgcc gccgcgaagt ccctgaaatt
      361 tcccggccag cgcgcgtcag gccccgccgc aaattttaga aaatgggcac gggcggcgcg
      421 accgaaggtc acgcgccggc cgtggcgctg ggcgtagcgc tcgagctcat actccaaatt
      481 gggctcgatg tcctcgggtc gctcgcgcag cgagggcatg cgaaacgtcc acaggtcgat
      541 gcgggcgagc aggtcctctc taaacttacc ctcgcgcacg caggcccgca ggtcgcggtt
      601 ggtgccggcg atcaattgaa aatcgctgtg gacctcgcgg tcgctgccga ccggcaggaa
      661 ggtgtggtcc tcgagcgcgc gcagcagcat cgcctgctcg tcgagcccga gctcgccgat
      721 ctcgtcgagg aagagcacgc ccccgtcggc gctcttgagc acgccaggcc ggtcccgcac
      781 cgcgccggtg aaggccccgc gcgtgtgccc gaacatggca gacatcgcgg cgtcgccccg
      841 cagcgtcgcg cagttgagct cgaagaagcg gcctgcgatc tggtggcgcg tctgcttgag
      901 ctcgaagatc ttgcgggcga ggcgggtctt gccggcgccg gtcggcccgg tcagcaagat
      961 cggcgagcgc gtcgcggtcg cgacccgctc gatctgatcg accaacgcgt tgaatgccgg
     1021 gttgcgggtc gcgatgccgg ccttgaggaa cgacaggccc tcgctgcgcg ccgcctgtac
     1081 gcgctcggcg atctggcggt agctggcgag gtcgaggtcg atcacgcgca ggtcgccggg
     1141 ctcgccgctg tgggccggcg tcggcggcga ggtctgcagc agcttgcccg gcaggtacct
     1201 cgcctccgtc aacaggaaca gacagatctg ctgcacatgc gtgcccgtcg tgatgtgcac
     1261 gaggtagtcc tcgtcgtcgg gacgaaacgg gtagccgtgc gcgaagtcca gcagggtcgc
     1321 aaacaccgac tcgaagtccc agggatccgc gaagctcagc gcgtgcgtgc aaacgcgcgt
     1381 ctccggtgac acgcttgcga tgtccgtcgc caccgtgccg gcgagctcct ggtgctgcgg
     1441 cgaatgcagc agctcgaggc ggtcgaccag gaagtcctcg cgccggcaaa ggtcgacggt
     1501 cggccgccag cgctcccagc gcgccttcgg gctggcgtcg agggaggtgc cgaggagccc
     1561 aaggaccaca gtcgcgcgcc gcacgccgtc acgatagaat tataaaaccc tcgccgcaag
     1621 gctaagtttt tccggtagtc acggcgcggc cgggtcagta agatgtggaa attactcgtg
     1681 cttgtgcgct cgcaaagaac ggcatcgggg ctgcttttga tctctttttg aaaagagaga
     1741 agagaac
//
LOCUS       DASWNJ010000502         3329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_644594, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000502 DASWNJ010000000
VERSION     DASWNJ010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644594"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VGB85_08520"
     CDS             <1..879
                     /locus_tag="VGB85_08520"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0134110.1"
                     /translation="LEMLFGIVRFVIDTISPDDPNDDALATLPRGAERVDVSLCVGSL
                     TRHLTISSKFPFTASRRDAWSIWMPVPDETEYPGFSSDHDVQSDRRDLNRLCRQPDGP
                     KLLYFPTDRAVTFPATRFKGPGNRSDDLEVAYRYRAPEEWLQSIEAILYNARWRDLNA
                     KERGKAGGGYFAAYEATMLRFFGDTKRFMWDDDGVLYVQTRDGTLHSLDELSSGEKQV
                     LLFGAELHRRWTPGSLILLDEPELHLHDAWLAKLWGFLGELQRERGGQVIIATQSNYL
                     FGLGEPGSRVILGRAP"
     gene            879..1700
                     /locus_tag="VGB85_08525"
     CDS             879..1700
                     /locus_tag="VGB85_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134111.1"
                     /translation="MDDRAIGQELERDLLRAAATGKVLYLEGPTDLLMLLGLLGREPP
                     TVVPEEGMPVDGVWVRGISGSKAVEQRIDVAGRLGFRDFRGIVDGDGKDLEALAATFD
                     PPFNGPLYHWKAYCLENLLTECSWPNWGPAPDWRSVLAAYIPYAALNRVVAAAQERWR
                     SLGVARFGRPIFDQPRRTTEEIRNQLHRDASDLRDLGDLVGLYDAEVNRCETALATSL
                     SHAHALVNGKWLVEVHAAEASRGSPERCRQAWAMHVGRTGGHPEVVAWWRRLQAA"
     gene            1827..2357
                     /locus_tag="VGB85_08530"
     CDS             1827..2357
                     /locus_tag="VGB85_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134112.1"
                     /translation="MKQLRREYELDICEPCAVNTDPVVASRLALGDRISTRQWTTRVR
                     TGNTTQTFHHMAIDVVPHAPLRVTATFTREGMWAKLKKLFFREVEVGDPMFDDFVHVG
                     TRDAEGTLALLGHSGVQSVLMSILADHEQVTFNDGAFEVYDQSIEEVYPGTALLAVCA
                     LLVHIEHIRSSRDIQS"
     gene            complement(2358..>3329)
                     /locus_tag="VGB85_08535"
     CDS             complement(2358..>3329)
                     /locus_tag="VGB85_08535"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0134113.1"
                     /translation="AQARVVAPPPPPAACPRCGAPQHLGRVTADMQLPGGGRRVFELT
                     YTGHSHALAREGGDHVVLISDVTERALADERMRQMNLDLINARDQAMAASRSKSAFLA
                     NMSHELRTPLNAIIGYSEILLEDGRDRPGAGDLKKIRTAGTHLLGIISSVLDLSKIEA
                     GKMGVDIEEFDVQVMLAQVLDTARPLVAANDNLLQHRVDPSVQTMVSDETKVRQILLN
                     LLSNAAKFTRQGRVELVVGRRMLGGFHVLEFAVHDTGIGIPQDQIARLFEDFQQADNS
                     TTRRYGGTGLGLTIVDRFCSMLGGTIEVESHVNRGSVFRVVLPERVR"
BASE COUNT          538 a         1048 c         1143 g          600 t
ORIGIN      
        1 ctcgagatgt tgttcgggat cgtccgcttt gtcatcgaca ccatctcgcc ggacgatcca
       61 aacgacgacg ctctcgccac gctgcctcgc ggagccgagc gcgtcgatgt ctcgctgtgc
      121 gtgggcagcc tgacgcgtca cctgacgatc agctccaagt tcccctttac tgcctcccgg
      181 cgagacgcgt ggtcgatctg gatgcccgtc ccggacgaga ccgagtatcc tggattctcg
      241 agtgatcacg acgttcagtc ggatcgacgg gacctcaacc ggctatgcag gcagcccgac
      301 ggcccgaagt tgctttactt cccgaccgac cgcgccgtga ccttcccagc gactcgcttc
      361 aagggtcctg gcaatcgtag cgacgatctc gaggtcgctt accgctatcg cgcgcccgag
      421 gagtggctgc agtcgatcga ggctatttta tacaacgccc gctggcgcga cctcaacgcg
      481 aaagagcgtg gaaaggccgg tgggggctac tttgcggcgt acgaggcgac gatgctgcgc
      541 ttcttcggcg acaccaagcg cttcatgtgg gacgacgacg gcgtcctgta cgtgcaaacc
      601 cgcgacggga cgttgcactc gctggacgag ctcagcagcg gcgagaagca ggtcctgctg
      661 ttcggcgcgg agctacatcg gcggtggacc ccgggttcat tgatcttgct tgacgagccc
      721 gagctccatc ttcatgacgc gtggctcgcg aaactctggg gattcctcgg agaactccag
      781 cgagagcgtg ggggtcaggt catcattgcg acccagagta attatctctt cggccttggc
      841 gagccgggga gtcgggtcat cctcgggagg gcgccctgat ggacgaccgc gcgatcggcc
      901 aggaattgga gcgcgacctc ctacgggcgg cagccaccgg aaaggtcctt tacctcgagg
      961 gcccgaccga tctgctgatg ctcctgggct tgctcggtcg cgagccgccg acggtcgtcc
     1021 cggaggaggg catgcctgtc gacggcgtat gggtgcgcgg catctcgggc agcaaggccg
     1081 tcgaacaacg cattgatgtg gcagggaggc tcggcttccg cgactttcgt gggatcgtcg
     1141 atggagacgg caaggatctc gaagcgctcg cagccacctt cgacccacca tttaacgggc
     1201 ccttatatca ctggaaggcc tattgtctcg agaacttgct gaccgagtgt agttggccaa
     1261 attggggacc agccccggat tggcggagcg tactcgctgc ttacattccc tatgcggccc
     1321 tgaatcgagt cgtcgccgca gctcaggagc gctggcgctc cctcggcgtg gcgaggttcg
     1381 gaaggcccat cttcgatcag ccgcggcgga ccaccgagga gatccgtaac cagctacatc
     1441 gtgatgcctc ggatctgcgg gacctcggcg acctcgtggg gttgtacgac gccgaggtga
     1501 accgctgtga gactgcgctt gcgacctcgc tgtcgcatgc gcacgcgctc gtcaatggca
     1561 agtggctcgt cgaggtacat gcggccgagg cttcgcgcgg cagcccggag cgttgtcgtc
     1621 aggcatgggc catgcacgtc ggtcgtacgg gaggtcaccc cgaagtcgtc gcgtggtggc
     1681 gacgcctcca ggcggcctga gcccggatcc tgccggcgat gcgcatggcc gagcagaggt
     1741 cgtgcgcgcc cgtgtattgg cctccgtgat accgtcgtcc tgtgacagag acagccccct
     1801 gtgccctctg cgcgcacgcc tggccggtga agcagctgcg acgcgagtac gagctcgaca
     1861 tctgtgagcc ctgcgcggtc aacacggatc cagtcgtcgc ctcgcgcctg gcgctcggtg
     1921 accgtatctc gacccggcag tggacgacgc gggtgcggac cggcaacacc acccagactt
     1981 ttcatcacat ggcgatcgac gtcgtgccgc atgcaccgct gcgcgtcacc gcgacgttca
     2041 cccgcgaggg catgtgggcc aagctcaaga agctgttctt ccgcgaggtc gaggtcggcg
     2101 atccgatgtt cgacgacttc gtccatgtcg ggacccgcga cgccgagggg accctcgcgc
     2161 tgctgggaca cagcggggtc cagtcggtgc tgatgagcat cctcgccgac cacgagcagg
     2221 tcactttcaa tgatggcgcg ttcgaggttt atgaccagtc gatcgaggag gtgtacccgg
     2281 gcaccgcact gctcgccgtc tgcgcgctgc tcgtgcacat cgagcacatc cgcagctcgc
     2341 gggacataca gagctgatca gcggacgcgc tcgggcagga ccacgcggaa caccgagccg
     2401 cggttgacgt ggctctccac ttcgatcgtg ccgccgagca tcgagcagaa tctatcgacg
     2461 atcgtcaggc cgaggccggt gccgccgtag cggcgggtgg tcgagttgtc ggcctgctga
     2521 aagtcctcga acaggcgcgc gatctggtcc tgggggatgc cgatgccggt gtcgtggacg
     2581 gcgaactcga gcacatgaaa gccgccgagc atgcggcggc cgacgaccag ctcgacgcgg
     2641 ccctggcggg tgaacttggc cgcgttgctg agcaaattca agaggatctg gcggaccttg
     2701 gtctcgtccg agaccatcgt ctgcaccgag gggtcgacgc ggtgttgtaa caagttgtcg
     2761 ttggcggcga ccagcgggcg ggccgtgtcg agcacctggg cgagcatgac ctggacgtcg
     2821 aactcctcga tgtcgacgcc catcttgccg gcttcgattt ttgaaagatc gaggacgctg
     2881 ctgatgatgc cgagcaggtg ggtgccggcg gtgcggatct tttttaaatc accggcgccg
     2941 gggcggtcgc ggccgtcctc gagcaggatc tcgctgtaac cgatgatcgc gttgagcggg
     3001 gtgcgcagct cgtggctcat gttggccagg aaggcgctct tggagcggct ggcggccatc
     3061 gcctggtcgc gcgcgttgat caggtcgagg ttcatctggc gcatgcgctc gtcggcgagc
     3121 gcccgctcgg tgacgtcgct gatcagcacg acgtggtcgc cgccctcgcg cgcgagcgcg
     3181 tggctgtggc cggtgtaggt cagctcgaag acccggcggc cgccgcccgg gagctgcatg
     3241 tcggcggtga cgcgaccgag gtgctgcggg gcgccgcagc gcgggcacgc ggccggcggc
     3301 ggcggcgggg cgacgacccg cgcctgggc
//
LOCUS       DASWNJ010000503         1981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_395905, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000503 DASWNJ010000000
VERSION     DASWNJ010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395905"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1377)
                     /locus_tag="VGB85_08540"
     CDS             complement(<1..1377)
                     /locus_tag="VGB85_08540"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134114.1"
                     /translation="MRKPSPASRPVRETSPSAGESAPGTGDVVGAVLAALRANRPLRV
                     VGAVGGYRGYLLAQAALDPRADGPVFTVAEDDSAARALALDAAFFMGAPQRELGVEGP
                     VLVVPEIDVSPYADTSADPRAVALRLAALHRLIDAQSDPPRLVIASLRSLMRRVIAPA
                     AFLGLCARWSRGDLIERDDAVATLERAGYLRVDVVEDPGTYAVRGGVIDIYVASLRFP
                     VRVELDDDEIERMRLFDPETQRSLRPIDHCAIHPVRETVATIEGPLRPRVLALADAIG
                     APSSKSRQVLENLEAGVDFFGQEALAPLFHGGMVPIWDYLPAGTRWFIDEPEGLAGLA
                     EEQHTACVEAHGRRMTARDLVAPPDSFFVPPELLAQRLASAPVIASRLAIADEPEDMS
                     QGAGEAVPKDSAEAAARLGVPTDRPIAWLALERPKVLRTQLEAARADRGGDVLRPLVE
                     RIRELEPPA"
     gene            1442..>1981
                     /locus_tag="VGB85_08545"
     CDS             1442..>1981
                     /locus_tag="VGB85_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134115.1"
                     /translation="MTRPRDKSALWEPAHAPAASQGAPAWYALLLAATTACASHPPAG
                     PATAPDPGDHRDPPAGAASESHDPGLSQTRAAEQTKKIMEAVAKARALPVTGAVEVAV
                     MRRAEIRDYAKASMYEHNSPEQIRLFTRIEASMGVLPVGADGEAILLDMLESGVMGLY
                     EPKNKTLYIGTHVGEGQLDM"
BASE COUNT          274 a          751 c          725 g          231 t
ORIGIN      
        1 ggccggcggc tcgagctcgc ggatgcgctc gacgagcggc cgcagcacat cgccgccacg
       61 gtcggcgcgg gccgcctcga gctgggtgcg tagcaccttg gggcgctcga gcgccagcca
      121 ggcgatcggt cggtcggtcg ggacgccgag gcgcgcggcc gcctcggcgc tgtccttcgg
      181 gacagcctcg cctgcccctt gcgacatgtc ctcgggttca tccgcgatcg ccagccgcga
      241 cgcgatcacc ggcgcgctgg cgagccgctg cgcgagcagc tcgggcggca cgaagaagct
      301 gtccggcggg gcgacgaggt cgcgcgcggt catcctgcgc ccgtgcgcct cgacgcaggc
      361 cgtgtgctgc tcctcggcga ggccggcgag gccctcgggc tcgtcgatga accaccgggt
      421 gcccgccggc aggtagtccc agatcgggac catgccgccg tggaacagcg gcgcgagggc
      481 ctcctgtccg aagaagtcga cgccggcctc gaggttctcg agcacctggc ggctcttgct
      541 cgacggcgcc ccgatcgcgt cggcgagcgc cagcacgcgc ggccgcagcg ggccctcgat
      601 cgtcgccacc gtctcgcgca ccgggtggat cgcgcagtga tcgatcggcc gcaggctgcg
      661 ctgcgtctcc gggtcgaaca gccgcatgcg ctcgatctcg tcgtcgtcga gctcgacgcg
      721 gaccgggaag cgcaggctcg cgacgtagat gtcgatcacg ccgccgcgga ccgcgtaggt
      781 cccgggatcc tcgacgacgt cgacgcgcag atagccggcg cgctcgagcg tcgcgaccgc
      841 gtcgtcgcgc tcgatcaggt cgccgcgcga ccagcgggcg cacagcccca aaaatgccgc
      901 cggcgcgatc acccgccgca tcagcgagcg caggctggcg atcaccaggc gcggcggatc
      961 gctctgcgca tcgatcaatc ggtgcagcgc ggccagccgc agcgcgaccg cccgcggatc
     1021 ggcgctggtg tccgcgtagg gcgagacgtc gatctccggc acgacgagca ccgggccctc
     1081 gaccccgagc tcgcgctgcg gcgcgcccat gaagaacgcg gcgtcgagcg cgagcgcccg
     1141 cgcggcgctg tcgtcctccg cgaccgtgaa caccggaccg tcggcgcgcg ggtcgagggc
     1201 cgcctgcgcc agcaagtagc cgcggtagcc gccgaccgcc cccaccaccc gcagcggccg
     1261 gttcgcgcgc agcgccgcca gcaccgcgcc gacgacatcg ccggtaccgg gcgccgactc
     1321 gccggcgctc ggcgaagttt cacggacggg tcggctcgct gggctcggct tgcgcatgcg
     1381 cggggctcgg ggacggtgcg ggaggctagc cgggccagcg cgcggcgagt aggctatgcc
     1441 ggtgacgcgc ccgcgagaca agtcggccct gtgggagccc gcacacgccc ccgctgccag
     1501 ccagggtgcc cccgcctggt acgcgctgtt gctggccgcc acgaccgcct gcgcgagtca
     1561 cccgccggcc ggaccggcga ccgcccccga cccgggcgac caccgcgacc ctcccgcggg
     1621 cgccgcgtcc gagtcgcacg accccggcct gagccagacc cgggccgccg agcagaccaa
     1681 gaagatcatg gaggccgtgg cgaaggcccg cgcgttgccg gtgacgggcg cggtcgaggt
     1741 cgcggtcatg cgccgggccg agatccgcga ctacgccaag gcctcgatgt acgagcacaa
     1801 ctcccccgag cagatccgcc tgttcacgcg catcgaggcg agcatgggcg tgctgccggt
     1861 cggcgccgac ggcgaggcga tcctgctcga catgctcgag agcggcgtga tgggcctcta
     1921 cgagcccaag aacaagaccc tgtacatcgg cacgcacgtc ggcgagggcc agctcgacat
     1981 g
//
LOCUS       DASWNJ010000504         2622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_9570, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000504 DASWNJ010000000
VERSION     DASWNJ010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9570"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1530)
                     /locus_tag="VGB85_08550"
     CDS             complement(<1..1530)
                     /locus_tag="VGB85_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0134116.1"
                     /translation="MAKPSSSYDASSIQVLEGVDHVRKRPGMYIGGVSKEGLHHLVWE
                     ILDNSVDEVINGHATTIEVKLDRDHKGITVIDNGRGIPVDLHPQLKVPAVVAVFTKLG
                     AGGKFDGESYKHSGGLHGVGAAVANALSESLTVKVWRDGSAWEAGFLRGVPDGKLKKL
                     GNARGSGTSVYLRPDPEIFGAKQTVFDPAVVRERLEAKSYLHKGLKIVFTDESTGKTE
                     TLFHAEGISEYLGKLVQVRGKPTVHPGIFYLERTDEVKLEVACAWTESSDDAMYSFVN
                     GVPTGDGGTHEQGFRAALGKAIRGYMTAHKLEPKGVTIALEDIREGMVAILSTYVLEP
                     QFQSQTKNRLNNPEVASQVESAIRPALEQWLHSNRSTAEGIVARVIISARARAASRAA
                     SQQVSRKGPGGVRLGLPGKLADCSERDPGKCELFLVEGDSAGGSAKMGRNRKTQAILP
                     LRGKVLNAEQASLAKVMENEELGNIVKVLGCGVGKDFVLENLRYAKVILLMDADSDGH
                     HI"
     gene            complement(1543..>2622)
                     /locus_tag="VGB85_08555"
     CDS             complement(1543..>2622)
                     /locus_tag="VGB85_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEY0134117.1"
                     /translation="EIQADAILETKLYRLAKLEIEKIRAELADKRAQAKKIEAILKSE
                     DKLWGVIRGELEDLAKNFPDARRTRISDEDVTEDFTAESFIVAEDAIVLITRDGWLKR
                     QRSINLASTRMREGDEALTLVGGSTKESVVLFTNKGSAYTLRIHDVPQSTGHGTPVQQ
                     LFKFRDGEKVIAAVGTDPRVMPEFAFPKPELGAEYEEPYPHYVAVSKQGMAMRFTLWP
                     HKDPSTSRGRMFGKLREGDEFIAVFKVYAEDDLCTLTKQGRSLCCNSQEVSLLGGPGL
                     GVILQKLDEDDEIVAAIPASVELVVEKATGGTQKIGGRGTKAVARGGKGVELWKRGAV
                     KRVILPEPVVPNLEAPVTTGASAKK"
BASE COUNT          338 a          903 c          887 g          494 t
ORIGIN      
        1 gatgtggtgg ccgtcgctgt cggcgtccat cagcaggatc accttcgcgt agcgcaggtt
       61 ctcgagcacg aagtccttgc cgacgccgca gccgagcacc ttgacgatgt tgccgagctc
      121 ctcgttctcc atcaccttgg cgaggctcgc ctgctcggcg ttgaggacct tgccgcgcag
      181 cggcaagatc gcctgggtct tgcggttgcg gcccatcttg gcggacccgc ctgcgctgtc
      241 gccctcgacg aggaacagct cgcacttgcc ggggtcgcgc tcggagcagt ccgcgagctt
      301 gccgggcagg ccgaggcgca cgccgccggg gcccttgcgc gagacctgct ggctcgccgc
      361 ccgcgaggcc gcgcgggcgc gcgccgagat gatgacgcgg gcgacgatgc cctcggcggt
      421 gctgcggttg gagtgcagcc actgctcgag cgccgggcgg atcgcgctct cgacctgcga
      481 cgccacctcg gggttattga ggcggttctt ggtctggctc tggaactggg gctcgagcac
      541 gtaggtgctg aggatcgcga ccatgccctc gcggatgtcc tcgagcgcga tcgtcacgcc
      601 cttgggttcg agcttgtggg cggtcatgta gccgcggatc gccttgccga gcgccgcgcg
      661 aaagccctgc tcgtgggtgc cgccgtcgcc ggtcggcacg ccgttgacga acgagtacat
      721 cgcgtcgtcg ctcgactcgg tccacgcgca ggcgacctcc agcttcacct cgtcggtacg
      781 ctcgagataa aaaatgccgg ggtgcaccgt cggcttgccg cggacctgca cgagcttgcc
      841 gaggtattcc gagatgccct cggcgtggaa cagggtctcg gtcttgccgg tgctctcgtc
      901 ggtgaagacg atcttgaggc ccttgtgcag gtagctcttg gcctcgaggc gctcgcgcac
      961 gaccgccggg tcgaacacgg tctgcttggc gccgaagatc tccgggtcgg gtcgcaggta
     1021 caccgaggtg cccgacccgc gggcgttgcc gagcttcttg agcttgccgt cgggcacgcc
     1081 gcgcaagaag ccggcctccc acgcggatcc gtcgcgccac accttgacgg tcagcgactc
     1141 ggacagcgcg ttcgcgaccg ccgcgccgac gccgtgcagg ccgccggagt gcttgtagct
     1201 ctcgccgtcg aacttgccgc cggcgccgag cttggtgaac accgcgacga cggcgggcac
     1261 cttgagctgc gggtgcaggt cgaccgggat cccgcggccg ttgtcgatga cggtgatgcc
     1321 cttgtggtcg cggtcgagct tgacctcgat cgtcgtcgcg tggccgttga tcacctcgtc
     1381 gaccgagttg tcgaggatct cccacacgag gtggtgcagg ccttccttgc tgacgccgcc
     1441 gatgtacatg ccggggcgct tgcggacgtg gtcgacgccc tcgagcacct ggatcgacga
     1501 cgcgtcgtaa ctgctgcttg gtttggccac gatggttctc cgctacttct tggccgatgc
     1561 gcccgtggtc acgggcgcct ctaaattcgg gacgaccggc tcgggcagga tcacccgctt
     1621 gaccgcgccg cgcttccaca gctcgacgcc cttgccgccg cgcgcgaccg ccttggtgcc
     1681 gcgcccgccg atcttttgtg tgccgcccgt cgccttctcg accacgagct cgacgctcgc
     1741 cgggatcgcc gcgacgatct cgtcgtcctc gtcgagcttt tgcaggatca ccccgaggcc
     1801 cggtccgccg agcaagctga cctcctggct gttgcagcac agcgagcggc cctgcttcgt
     1861 cagcgtgcac aggtcgtcct cggcgtagac cttgaacacc gcgatgaact cgtcgccctc
     1921 gcgcagcttg ccgaacatgc ggccgcggct ggtgctcggg tccttgtgcg gccacagcgt
     1981 gaagcgcatc gccatgcctt gcttgctgac cgcgacgtag tgcgggtacg gctcctcgta
     2041 ttcggcgccg agctcgggct tggggaacgc gaactccggc atcacgcgcg gatccgtgcc
     2101 gaccgccgcg atcaccttct cgccgtcgcg gaacttgaac agctgctgca ccggcgtgcc
     2161 gtggccggtc gactgcggga cgtcgtggat ccgcagcgtg taggcggagc ccttgttggt
     2221 gaacagcacc acgctctcct tggtgctgcc gccgacgagc gtcagcgcct cgtcgccctc
     2281 gcgcatgcgc gtgctggcga ggttgatcga ccgctggcgc ttgagccagc cgtcgcgcgt
     2341 gatcagcacg atcgcgtcct cggcgacgat gaagctctcc gccgtgaagt cctcggtgac
     2401 atcctcgtcg gagatgcggg tgcgccgcgc gtccgggaag ttcttggcga ggtcctcgag
     2461 ctcgccgcgg atgacccccc acagcttgtc ctcgctcttg aggatcgcct cgatcttctt
     2521 ggcctgcgcg cgcttgtcgg ccagctcggc gcggatcttc tcgatctcga gcttggcgag
     2581 ccgataaagc ttggtctcga ggatcgcgtc ggcctggatc tc
//
LOCUS       DASWNJ010000505         4001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_609215, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000505 DASWNJ010000000
VERSION     DASWNJ010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609215"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..3546
                     /locus_tag="VGB85_08560"
     CDS             40..3546
                     /locus_tag="VGB85_08560"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0134118.1"
                     /translation="MPTTPHGATPGDSEGQRMIKDVIKAQLFPGEAVPVMIGRFRVLR
                     PLGAGGMSVVYAAYDERLDRKVAVKLLKGGGSPDNQARMLREAQAMAKLSHQNVVAVH
                     EVGTWGEQVYIAMEFVKGMDLRAWLAQEPHAWPEVLEVFTAAGHGLSAAHRAGLVHRD
                     FKPANVLLGDDGRVRVVDFGLVRQSDEAHAPGPGASMGGAPIISLHTTLTATGAILGT
                     PAYMSPEQHEGRAADARSDQFSLCVALWEGLYGAPPFAGASLPALADAVCNGRVRPVP
                     EDSRVPAWLRRVVLRGLSVDPAARWPSMDELLDELARDPDRLRRARLRFGLIAGAMTA
                     VVLTFAWLASSQADVARVAQRAEAAADRQRRQAEEQQVTAEVARDEAMEKLEQMLAEA
                     EAARAGLERALQTADAERRRAENESAAAEAQRMRAEAQRREAEAARREAEHQTARAVQ
                     EARRARDATRLAQALGAAEDDPTTVLALLRETEDPAGTPGWVPAAVDTLLQPVSLAVL
                     RLHRGSVHAAIFSADGRSVATAGDDHVAALWRWGSGVAPQRREHAAPVKFLAFGPGEI
                     WASGASDGEVLVGGAAPRRLAHGGELRAMSLSPDGRRLATASADGRLRVWDTSDGSAR
                     ELPGHTAAVLSVMFDRAGRRLVSAAADGTARVWDVTAGPPRILQHPGAVRIAVFHPDG
                     RRVATGAQDGEVRVWPIDGEPTAAPSVHHGHTGEVVALAFHPEGDALLSASLDGTTRV
                     WARKPGQKDRVLRGHAGRVYSAQWSPDGDRIVTASQDGTARVWPAQGGPPRVLRGHAE
                     ELGAAGFDPGGDHVVTAGRDGSVRVWQVRGSGEEVPVLRSGGRPTLLAWTPDGEHLLS
                     GRSDGVVHLWQPGSTAPPRRLAEPGPTPLWLGASDSELRAIRNDGVLLRWPLAGGPAT
                     TRVLIPATPAASAASLTADGSRAALATADALWLLSPGTGERQALRGHTGTIFAAQFAP
                     DGASLLTGASDRSGRLWPPGSLSSGTDARVLAPHSGGVTAVAFRGDGTMVATAGWDKR
                     ARVWPAAGGDPVVLAGHTGPVWAVAFSPDGARVATGADDRSVRVWPVDGRDEPVVLSG
                     HAGPVRATRFRPDGALLASAGDDGTIRLWNADLSAQGLQARLRRASPVCLSARQRTQL
                     LGEDPATAEQVASACQSTPPAP"
     gene            complement(3568..>4001)
                     /locus_tag="VGB85_08565"
     CDS             complement(3568..>4001)
                     /locus_tag="VGB85_08565"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEY0134119.1"
                     /translation="RNPQFGAVALARSLADADIMYAQIRALGGRRRPAPDSPNGAWRN
                     ESFRGYADYMASPEFMLGLAELLARAELAPTAIMCAEAVPWRCHRSLIADALIARGVV
                     VRDIFAVDQVRPHALSPMAVVVDGVVRYPPTPAAQLDLLAR"
BASE COUNT          487 a         1603 c         1481 g          430 t
ORIGIN      
        1 ttcgatcccc atcccagccg cgacgacacc gatcccgcga tgccgacgac gccgcacggc
       61 gccacgccgg gcgacagcga gggccagcgg atgatcaagg atgtcatcaa ggcccagctg
      121 ttccccggcg aggccgtgcc ggtgatgatc ggccggtttc gggtgctgcg gccgctcggg
      181 gccggcggca tgagcgtggt gtacgccgcc tacgacgagc gcctcgatcg caaggtcgcc
      241 gtcaagctgc tcaagggcgg cggatccccg gacaaccagg cccgcatgct gcgcgaggcc
      301 caggcgatgg ccaagctctc gcaccagaac gtcgtcgccg tgcacgaggt cggcacctgg
      361 ggcgagcagg tctacatcgc catggagttc gtcaagggca tggacctgcg cgcctggctg
      421 gcccaggaac cacacgcctg gcccgaggtg ctcgaggtgt tcaccgccgc cggccacggc
      481 ctgtccgccg cgcaccgggc cggcctggtc caccgcgact tcaagccggc caacgtgctg
      541 ctcggcgacg acggtcgggt gcgcgtcgtc gacttcgggc tcgtgcgcca gagcgacgag
      601 gcccacgcgc cgggccccgg ggcgagcatg ggcggggcgc cgatcatcag cctgcacacg
      661 accctgaccg cgaccggggc gatcctcggc acgcccgcgt acatgtcgcc cgagcagcac
      721 gagggccgcg ccgccgacgc gcgcagcgat cagttcagcc tgtgcgtcgc gctgtgggag
      781 ggcctctacg gcgcgccgcc gttcgccggc gccagcctgc ccgcgctcgc cgacgccgtc
      841 tgcaacggcc gcgtgcgacc tgtccccgag gacagccgcg tgcccgcctg gctgcgccgc
      901 gtggtcctgc gcgggctctc ggtcgacccc gccgcgcgct ggccgagcat ggacgagctg
      961 ctcgacgagc tcgcccgcga ccccgaccgc ctgcgccgcg cccggctgcg cttcggcttg
     1021 atcgccggcg cgatgacggc ggtggtcctg accttcgcgt ggctcgccag cagccaggcc
     1081 gacgtcgccc gcgtcgccca gcgggccgag gccgccgccg accgccagcg ccgccaggcc
     1141 gaggagcagc aggtcaccgc cgaggtcgcc cgcgacgagg cgatggagaa gctcgagcag
     1201 atgctcgccg aggccgaggc cgccagagcc ggcctcgagc gcgcgctcca aaccgcggac
     1261 gccgagcgcc gccgggccga gaacgagagc gccgcggccg aggcccagcg catgcgcgcc
     1321 gaggcccagc gccgcgaggc cgaggcggcc cgccgcgagg ccgagcacca gaccgcgcgc
     1381 gcggtgcagg aggcccgccg ggcccgcgac gcgacgcgcc tggcccaggc gctcggggcc
     1441 gccgaggacg acccgacgac cgtgctcgcg ctgctgcgcg agaccgagga ccccgccggc
     1501 acgcccggct gggtcccggc cgccgtcgac accttgctgc agccggtcag cctcgccgtg
     1561 ctgcgcctgc accgcggcag cgtgcacgcg gcgatcttca gcgccgacgg ccgcagcgtc
     1621 gcgaccgcgg gcgacgacca cgtcgccgcg ctgtggcgct ggggctcggg cgtcgccccg
     1681 cagcggcgcg agcacgcggc cccggtcaag ttcctggcct ttgggccagg tgagatctgg
     1741 gccagcggcg ccagcgacgg cgaggtcctc gtcggcggcg ccgcgccgcg ccggctcgcc
     1801 cacggcggcg agctgcgggc catgtcgctg tcaccggacg gccggcggct cgcgaccgcg
     1861 tcggccgacg gccgcctgcg cgtgtgggac acgagcgacg gcagcgcgcg cgagctgccc
     1921 ggccacaccg cggctgtcct ctcggtcatg ttcgatcgcg caggtcgccg gctcgtcagc
     1981 gccgccgccg acggcaccgc ccgcgtgtgg gacgtgaccg ccggtccgcc gcggatcttg
     2041 cagcacccgg gcgcggtgcg gatcgccgtg tttcaccccg acggccgccg cgtcgccacc
     2101 ggcgcgcagg acggcgaggt ccgcgtgtgg ccgatcgacg gcgagccgac ggccgccccg
     2161 agcgtgcatc acgggcacac cggcgaggtc gtcgcgctcg cgttccaccc ggagggcgac
     2221 gccctgctgt cggcctcgct cgacggcacc acgcgcgtgt gggcccgcaa acctggccaa
     2281 aaggacaggg tcctgcgcgg ccacgccggc cgggtctaca gcgcccagtg gagccccgac
     2341 ggcgaccgca tcgtcaccgc ctcgcaggac ggcacggcgc gcgtgtggcc ggcgcagggc
     2401 ggccccccgc gggtgctgcg aggccacgcc gaggagctcg gcgccgccgg cttcgacccc
     2461 gggggcgacc acgtcgtcac cgccggccgc gacggcagcg tgcgggtgtg gcaggtgcgc
     2521 ggcagcggcg aggaggtccc ggtgctgcgc tcgggcggcc gtccgaccct gctcgcctgg
     2581 acccccgacg gcgagcacct gctcagcggc cgcagcgacg gcgtggtcca cctctggcag
     2641 cccgggagca ccgcgccgcc gcgtcgcctc gccgagcccg gcccgacccc gctgtggctc
     2701 ggcgccagcg acagcgagct ccgggcgatc cgcaacgacg gcgtcctgct gcgctggccg
     2761 ctcgcgggcg gccccgcgac cacccgcgtg ttgatccccg cgaccccggc cgccagcgcc
     2821 gcgagcctga ccgcggacgg cagccgggcc gccctcgcca ccgccgacgc gctgtggctg
     2881 ctgtccccgg ggacaggtga gcgccaggcc ctgcgcggcc acaccggcac gatcttcgcc
     2941 gcccagttcg cgcccgacgg cgcgtcgctg ctgaccggcg cgagcgatcg cagcggtcgc
     3001 ctgtggccgc ccggcagcct gtcctcgggg acagacgccc gtgtgctcgc cccgcactcc
     3061 ggcggcgtca cggcggtcgc gtttcgcggc gacggcacca tggtcgcgac cgcgggctgg
     3121 gacaagcgcg cccgcgtgtg gccggccgcc ggcggggacc cggtcgtgct cgccggccac
     3181 accggcccgg tgtgggcggt cgccttcagc ccggacggcg cccgcgtcgc gaccggcgcc
     3241 gacgaccgca gcgtgcgcgt ctggccggtc gacggccgcg acgagccggt cgtgctctcg
     3301 ggccacgccg gtccggtacg cgcgacccgg tttcgccccg acggcgccct gctggccagc
     3361 gccggcgacg acggcacgat ccgcctgtgg aacgcggatc tcagcgcgca gggcctgcaa
     3421 gcccgcctgc gccgcgcctc gcccgtgtgc ctgtcggccc gccagcgcac ccagctgctc
     3481 ggcgaggacc cggccaccgc ggagcaggtc gccagcgcct gccagtcgac cccgccggcc
     3541 ccctgagacc tgtccccggg gacagcctca gcgcgcgagc agatcgagct gcgccgccgg
     3601 ggtcggggga tagcggacca cgccgtccac cacgaccgcc atcgggctca gcgcgtgcgg
     3661 ccggacctgg tcgaccgcga agatgtcgcg caccaccacc ccgcgggcga tcaaggcgtc
     3721 ggcgatcaac gagcgatggc agcgccacgg caccgcctcc gcgcacatga tcgccgtcgg
     3781 ggccagctcg gcgcgggcca gcagctccgc gaggccgagc atgaactccg ggctcgccat
     3841 ataatcggcg tagccgcgaa acgactcgtt gcgccaggcc ccgttcggcg agtcgggcgc
     3901 gggccgccgc cgcccgccga gcgccctgat ctgcgcatac atgatgtccg cgtccgcgag
     3961 cgatcgggcc agcgccaccg ccccgaattg cgggttgcgc c
//
LOCUS       DASWNJ010000506         3945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_185866, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000506 DASWNJ010000000
VERSION     DASWNJ010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185866"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1221
                     /locus_tag="VGB85_08570"
     CDS             187..1221
                     /locus_tag="VGB85_08570"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY0134120.1"
                     /translation="MHARLVLLLSLALPGCFDGGPLFDSVGSESTGSDPTGDGTSTGA
                     PTSTTGEGPTSTGAGTSTGEPVTTTQSGSTTEAGSTTTGEGTTGEASSGTTGDDLLDE
                     CPRLRVDVAPDPTLNVRSTPSTEFEPIGSLVHGAIVETVAAVQGESIDGNTLWYQIAA
                     PAGYVSGQFVECTQDVPPPPPDGYYLPLACGMSAKITQGNNGGTSHSGKAQYAFDFGL
                     ALNTPLHAMADGTVLHVYAETGPGDPCYDGGGPDCFPHGNLVVLLHGDGSTTLYKHLN
                     KVDVALEQFVPRGGVVGLSGSTGYSTGPHAHVMRMENCGLNNCQSIPLEFIEAGVPVQ
                     GQTVTSQNCP"
     gene            complement(1199..2086)
                     /locus_tag="VGB85_08575"
     CDS             complement(1199..2086)
                     /locus_tag="VGB85_08575"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HEY0134121.1"
                     /translation="MRRTGLSTMLLAYVAATLLAAIVWWFTPALAPWQRTLCAGLAAT
                     GLLWLVGLVRGNPSVFDPYWSVAPMLVAMAWSHDHASSDASVTRQFVVLCLVCAWGLR
                     LTYHWWRTFPGLDHEDWRYTDMRETSGRLFPLVNLVVIHFVPTLMLLLACAAMYPAVV
                     TGTRPLGFLDLFAATWTLSAILLEARADHEMRAFRSRPREAGTVYQSGVWAWLRHPNY
                     LGELSFWWGLFLFSLAADPTARWAAAGPLVITAMLVLYSAPALDRRMLARYPAYRDYM
                     ARVPSLLPRRPAVTGSSAT"
     gene            complement(2132..2278)
                     /locus_tag="VGB85_08580"
     CDS             complement(2132..2278)
                     /locus_tag="VGB85_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134122.1"
                     /translation="MLKVLLERIPVQSGPKPAATLLVNLYMREYPAPSDEHRALVARI
                     LGRT"
     gene            complement(2351..2968)
                     /locus_tag="VGB85_08585"
     CDS             complement(2351..2968)
                     /locus_tag="VGB85_08585"
                     /inference="COORDINATES: protein motif:HMM:NF025844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4435 domain-containing protein"
                     /protein_id="HEY0134123.1"
                     /translation="MSRYPEGDALLAVCRAGLGKLVIVEGETEEDDPYFYGRWFGDLA
                     RELTFIPQNGHEKVLKAVQDLRARLSPAREVYGIRDRDFSDDSAWQAQANSIPADGVL
                     FTRWYTLENYLLEPEGWFRVVSAMHRRPPGPWDTEAAVAAQIADAYRECLPLAAWNFV
                     VRSECQRLPKAGLRHVEHPKALPPQPHQALAEWGRARDAPRSLDE"
     gene            complement(2965..>3945)
                     /locus_tag="VGB85_08590"
     CDS             complement(2965..>3945)
                     /locus_tag="VGB85_08590"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0134124.1"
                     /translation="NFGPNAGAVSFVDPLTQQVRPLSVLVGSNGCGKTTVLDTIEGLL
                     GLIGRPSRDICTEIRRTGHADISVEFSKDEQHQSVYDPPALVQFGQGPAEYMIFSDGK
                     LEGPPALMQLLRISQWTREMEEGWYPVRGGLIYFPHHRWVEYEEKGSITEPSPARSWS
                     FRFTPESGWTGSLSQLWVWQNYLDLEQGREGRPNLTPFVEAIETILGRGRRVVIRSGR
                     VYIEQPGREPVQPHQLPSGEQQILTLFGEIIRHMRPGGVLIIDEVEISLHPALQRVVL
                     HHLRELAARYDLQIILTTHSMEIVAAVAPDELVNLDDMVLQERAQSGGAS"
BASE COUNT          649 a         1285 c         1451 g          560 t
ORIGIN      
        1 cacgagatgg cacagtggcc gtcgatcggc cacgcgaact gcatcgaggt cgacccgaag
       61 acgaagggtt accgtgcggt ggccgacgtc ggtcgcgacg gcggcagcgc ggtcgcctac
      121 tgaccccgag ggtgggggcg cggcgcggct tgccgggtcc ggggtgatcc gcgaagatgc
      181 cggcgcatgc acgcccggct ggtcctcctg ctctcgctcg cgctccccgg ctgcttcgac
      241 ggcgggcctc tgttcgacag cgtcggctcc gagtcgaccg ggtccgaccc cacgggagac
      301 ggcacgagca caggcgctcc gacgagcacg acgggcgagg ggccgacgag cacgggcgcc
      361 gggacgtcca ccggcgagcc cgtgacgacg acgcagtcgg ggtcgacgac ggaggccggg
      421 tcgacgacga ccggcgaggg gacgaccggc gaggcgagca gcgggacgac cggcgacgac
      481 ctgctcgacg agtgcccgcg cctgcgcgtc gatgtcgcgc cggatccgac gctcaacgtg
      541 cgctcgacgc catcgacgga attcgagccg atcggatcgc tcgtgcacgg ggcgatcgtc
      601 gagacggtcg ccgcggtgca gggcgagtcg atcgacggca acacgctctg gtatcagatc
      661 gcggcgccgg ccggctacgt gtcggggcag ttcgtcgagt gcacgcagga cgtcccgccg
      721 ccgccgccgg acggctacta cctgccgctc gcgtgtggca tgagcgcgaa gatcacccag
      781 ggcaacaacg gcggcaccag ccactccggc aaggcccagt acgccttcga cttcgggctc
      841 gcgctcaaca caccgctgca cgcgatggcc gacggcacgg tgctgcacgt gtacgccgag
      901 accgggccgg gcgacccctg ctacgacggc ggcggcccgg actgcttccc ccacggcaac
      961 ctggtcgtgt tgctgcacgg cgacggcagc acgacgctgt acaagcatct caataaggtc
     1021 gacgtggcgc tcgagcagtt cgtgccgcgc ggcggggtgg tcgggctgtc gggctcgacc
     1081 ggctactcga cggggccgca cgcgcacgtg atgcgcatgg agaactgcgg gctcaacaac
     1141 tgtcagtcga tcccgctcga gttcatcgag gccggggtgc cggtgcaggg gcagacggtc
     1201 acgtcgcaga actgcccgtg accgcggggc ggcgcggcag cagcgagggc acgcgggcca
     1261 tgtagtcgcg gtaggccggg tagcgcgcga gcatgcggcg gtcgagcgcg ggggcgctgt
     1321 agagcacgag catcgcggtg atcaccagcg ggccggccgc ggcccagcgg gcggtcgggt
     1381 cggccgcgag cgagaagagg aacaggcccc accagaacga cagctcgccg aggtagttgg
     1441 ggtggcgcag ccaggcccag acgcccgact ggtagacggt gccggcctcg cgggggcggc
     1501 ttcggaaggc tcgcatctcg tggtcggcgc gggcctcgag caggatcgcg gacagggtcc
     1561 aggtcgcggc gaacaggtcg aggaacccga gggggcgggt gccggtgacg accgcggggt
     1621 acatcgcggc gcacgcgagc agcagcatca gcgtcgggac gaagtgaatg acgacgaggt
     1681 tgaccagcgg gaacaggcgg ccgctggtct cgcgcatgtc ggtgtagcgc cagtcctcgt
     1741 ggtcgaggcc ggggaaggtg cgccaccagt ggtaggtcag gcgcaggccc cacgcgcaca
     1801 cgaggcacag cacgacgaac tgacgcgtga cgctggcgtc gctgctcgcg tggtcgtgcg
     1861 accacgccat agcgaccagc atcggcgcga cggaccagta cgggtcgaac acgctcgggt
     1921 tgccgcgcac gagcccgacg agccacagca ggccggtcgc ggcgaggccg gcacacagcg
     1981 tgcgctgcca cggcgcgagc gcgggcgtga accaccagac gatcgcggcc agcagcgtgg
     2041 cggccacgta ggcgaggagc atggtcgaga ggccggtgcg tcgcatggcc cgggtttcgc
     2101 acggtcgcgc acgcgaggca accgcgggct gtcaggtgcg cccgaggatg cgtgcaacga
     2161 gcgcccggtg ctcgtcggag ggggccgggt actcacgcat gtagagattc acgagcagcg
     2221 tcgcggcggg tttcgggccg gactgcaccg ggatgcgctc gagcaggacc ttgagcacgg
     2281 ccttgccggt gacccagcat ggcagctccg ccggtgaggc ggtgctcagc cattcgagtc
     2341 gctccgcgta tcactcgtcg agcgagcgcg gtgcgtcccg ggcccggccc cactcggcga
     2401 gcgcctggtg cggctgcggt gggagggcct tcgggtgttc gacatgacga agtccggcct
     2461 tcgggagccg ctggcattcg gaacgcacga cgaagttcca ggcagcgagc ggcagacatt
     2521 cgcggtaggc gtcggcgatc tgggcggcga cggcggcctc cgtgtcccac ggcccgggcg
     2581 ggcggcgatg catcgccgag acgacccgaa accacccctc gggctccagc aaatagttct
     2641 cgagcgtgta ccagcgcgtg aacagcacgc cgtcggccgg tatcgagttt gcctgcgctt
     2701 gccaggcgga gtcgtcggag aagtcgcggt cgcggatgcc gtagacctcg cgcgcaggag
     2761 acaggcgggc gcgcagatcc tggaccgcct tcaggacctt ctcgtggccg ttttgcggga
     2821 taaaggtcag ctcgcgcgcg aggtcgccga accagcgacc ataaaagtac ggatcgtctt
     2881 cttcagtctc gccctcgacg atcacgagct tgccgaggcc cgcacggcac accgccagca
     2941 acgcgtcgcc ctcggggtaa cggctcacga agcaccgccg ctctgggccc gctcctgcaa
     3001 gaccatgtcg tcgaggttca cgagctcgtc cggcgcgacc gcggcgacga tctccatcga
     3061 gtgcgtcgtc aggatgatct ggaggtcgta gcgcgccgcg agctcgcgca ggtggtgcag
     3121 cacgacgcgc tggagggcag ggtgcaggct gatctcgacc tcgtcgatga tcaggacgcc
     3181 gccgggccgc atgtggcgga tgatctcgcc gaacagggtc aggatctgtt gttcacccga
     3241 ggggagctga tgcggctgga caggctcgcg cccgggctgc tcgatgtaca cgcggccgct
     3301 gcggatcacg acacgacggc cacggccgag gatggtctcg atcgcctcga cgaacggggt
     3361 caggttgggg cggccctcgc ggccctgctc gagatcgagg tagttctgcc acacccacaa
     3421 ctgggagagg ctgccggtcc acccgctctc cggagtgaag cgaaacgacc aacttcgcgc
     3481 tggcgatggc tctgtaatgg accccttctc ctcatactcg acccagcggt gatgcgggaa
     3541 atagatcagc ccgccgcgaa ctggatacca gccctcttcc atttctcgtg tccattgcga
     3601 gattcttaag agctgcatca gtgcgggagg gccctctagc ttgccatcac tgaaaatcat
     3661 gtactcggcg ggcccttgcc cgaattgcac gagtgccggg gggtcgtaaa cgctttggtg
     3721 ctgctcgtcc ttggagaact cgacgctgat gtcggcgtgg ccggttcttc gaatttcagt
     3781 gcagatatcc cgagaagggc gaccgatcag gcccaggagg ccctcgatcg tgtcgagcac
     3841 ggtcgtcttg ccgcagccgt tcgatccgac gagcactgac agcggccgga cttgttgcgt
     3901 cagcgggtcg acgaacgata ccgctccggc gttcgggccg aagtt
//
LOCUS       DASWNJ010000507         2508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_292250, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000507 DASWNJ010000000
VERSION     DASWNJ010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292250"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1324
                     /locus_tag="VGB85_08595"
     CDS             131..1324
                     /locus_tag="VGB85_08595"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0134125.1"
                     /translation="MTAMFDLVSDDMRRDPFPLYAHLRSAAPVFSPPGSGLWLLLDHE
                     SVKRALGDPATFSSAVMLPTGKAPDWLSFSDPPRHTKMRALIMRAFTPRSVALLEPRV
                     RELANELLDEHIESGEMDLAVDYAGPLPMLVIAEMVGMPADDRAMLMRWSEVIMDLSH
                     AVAGGATAARAIEAHAAAKEEMQDYLDRLTAQRRAAPRDDLLTRLVEAEVDGEHLTPS
                     DILGFFQLLLAAGTETTTNLIDNTVLCLCEHPDQRARLAGAPELLTSAIEEVLRYRTP
                     AQMVFRQTLRAVEMHGQHIPAGQFVIAVTGAANRDPKVFVEPDRFDIARDPNPHIAFG
                     HGIHFCIGAALSRLEGRVAVGELLRRAPDLRLASEAPWQPRRALIAHGPVHLPICFAP
                     GPRGG"
     gene            complement(1327..>2508)
                     /locus_tag="VGB85_08600"
     CDS             complement(1327..>2508)
                     /locus_tag="VGB85_08600"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134126.1"
                     /translation="ALLGLALTTACNDGYRPPPPTQHPVTPAPRPTPAPAPKPAPTPA
                     ARPAPPADPMVALAARGRHTCALRKSGALACWGNNSDGQLGDGGRVDQPRGVPVAGLS
                     DMSEVATGLAFSCGRKASGQVMCWGNNEDGQLGDGRGAKPGSRSASPVAVKGLADATQ
                     LSLGDSHACALRRSGTVSCWGLGGDGQLGAVIERSAQPLAIPGTAQTVEVASGSNHVC
                     ARKQNGAVFCWGRNTEGQLGDGASGSKIKPVAVKGLTDATLLVAGGNHTCARRKSGAV
                     VCWGNNSQGQLGDGTTGTFAQRTPVIVAGLTNPTELALGKEHSCARSGDRVSCWGGNS
                     SGQLGDGSNSGHPKPSPVTRLTGAADLALGTAHSCALQGTGNVTCWGSDEHRALGPRR
                     L"
BASE COUNT          320 a          859 c          953 g          376 t
ORIGIN      
        1 gtcgttccgc gggcccgcgg accgcgcagg tcgtcgcgcg cgggcacgca atgggtcgcg
       61 tacgatgtcc acggggacgc gcgcggcggg cgtggacccc cggcgcaggc ggcgacaatt
      121 tcgtgcggca atgacggcga tgttcgacct ggtctccgac gacatgcgtc gcgatccctt
      181 ccctctgtat gcgcacctgc ggtcggccgc gccggtattt tcgccgccgg ggtcggggtt
      241 gtggttattg ctcgaccacg agagcgtgaa gcgggcgctc ggcgacccgg cgaccttcag
      301 ctcggcggtg atgttgccga ccggcaaggc gcccgactgg ctcagcttct cggacccgcc
      361 gcgccacacg aagatgcggg cgctgatcat gcgggcgttc acgccgcgct cggtcgcgct
      421 gctcgagccg cgggtccgcg agctggcgaa cgagctgctc gacgagcaca tcgagtcggg
      481 cgagatggac ctcgccgtcg actacgccgg cccgctgccg atgctggtga tcgcggagat
      541 ggtcggcatg ccggccgacg accgggcgat gctgatgcgc tggagcgagg tgatcatgga
      601 cctcagccac gcggtcgccg gcggggccac ggcggcgcgg gcgatcgaag cccacgcggc
      661 ggccaaggag gagatgcagg actacctcga tcgcctgacc gcgcagcgac gggccgcgcc
      721 gcgcgacgac ctgctgacgc gcctcgtcga ggcggaggtc gacggcgagc acctgacgcc
      781 ctccgacatc ctcgggttct ttcaattgtt gctggccgcc ggcaccgaga cgacgaccaa
      841 cctgatcgac aatacggtgc tgtgcctgtg cgagcacccg gaccagcgag ctcgcctggc
      901 cggcgcgccg gagctgttga ccagcgcgat cgaggaggtg ctgcgctacc ggacgccggc
      961 gcagatggtg tttcgtcaga cgctgcgcgc ggtcgagatg cacggccagc acatccccgc
     1021 gggccagttc gtgatcgcgg tgaccggcgc ggcgaaccgc gacccgaagg tgttcgtcga
     1081 gccggatcgc tttgatattg cgcgtgaccc gaatccacac atcgcgttcg gccacggcat
     1141 ccatttttgt atcggcgcgg cgctgtcgcg gctcgagggg cgcgtcgcgg tcggcgagct
     1201 gctgcgtcgc gcgccggatc tacggctcgc gagcgaggcg ccgtggcagc cgcgcagggc
     1261 cctcatcgcg cacggaccgg tccacctgcc gatctgcttc gcgccggggc cgcgcggcgg
     1321 ttgatgtcac aggcggcgtg gcccgagggc gcggtgctcg tcgctgcccc agcaggtcac
     1381 gttgccagtg ccctgcaggg cgcagctgtg ggctgtcccg agggccaggt cggcggcgcc
     1441 ggtcaggcgg gtgacggggc tcggcttcgg gtggccactg ttgctgccat caccgagctg
     1501 gccggaggag ttgccacccc agcaggacac gcggtcgccg gagcgcgcgc agctgtgctc
     1561 cttgccgagc gcgagctcgg tcgggttcgt gagtccggcc acgatcacgg gggttcgctg
     1621 ggcgaaggtg ccggtcgtgc cgtcgccgag ctggccctgg ctgttgttcc cccagcacac
     1681 gacggcgccg ctctttcgtc gggcgcaggt gtggttaccg ccggcgacca ggagggtcgc
     1741 gtcggtcagg cccttcacgg cgaccggctt gatcttgctg ccggacgcgc cgtcgccgag
     1801 ctggccctcg gtgttgcgcc cccagcaaaa caccgcgccg ttctgcttac gcgcacacac
     1861 atggttgctg ccgctggcga cctccaccgt ctgcgcggtg ccggggatcg ccaggggctg
     1921 cgcgctgcgc tcgatgaccg cgccgagctg gccgtcgccg ccgaggcccc agcagctgac
     1981 agtgccggaa cgtcgcaggg cgcacgcgtg gctgtcaccg agtgagagtt gagtcgcgtc
     2041 ggcgaggccc ttgacggcga ccggactggc actacgcgac cccggcttcg cgccgcggcc
     2101 gtcgccgagc tggccgtctt cattattacc ccagcacatc acctggccag aggccttgcg
     2161 gccgcacgag aacgcgaggc cggtggcgac ctcggacatg tccgaaaggc cagcgaccgg
     2221 gacgccccgc ggctggtcga cgcggccgcc gtcgccgagc tggccgtcgc tgttgttccc
     2281 ccagcatgcg agcgcgccgg acttgcgcag ggcgcaggtg tggcgccccc gcgcagcgag
     2341 cgcgaccatc gggtccgcgg gtggcgcagg cctggcggcg ggcgtggggg ccggttttgg
     2401 cgcgggcgcg ggcgtgggcc ggggcgcagg cgtgacgggg tgctgcgtgg gaggaggcgg
     2461 gcggtagccg tcgttgcacg cggtggttag cgcgagccca agcagggc
//
LOCUS       DASWNJ010000508         7522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_221015, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000508 DASWNJ010000000
VERSION     DASWNJ010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7522
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221015"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2650)
                     /locus_tag="VGB85_08605"
     CDS             complement(<1..2650)
                     /locus_tag="VGB85_08605"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0134127.1"
                     /translation="MANDKLRDNSLVHLSLVRPVTMLMVLLSFIVIGIVALVNIPIEL
                     IPSGFSPPFMQVEVPYANATAQDVEDRITRPLEQALSTTPGLDELSATSRADRASLSL
                     VFESDTDMNVAYREVRDRVARARPDLPADVEKVRIRKESGAGIPVAFYGITWDETVEL
                     PRDRLQKHMVRAIERIDGVGLVNLWGQEDREIRIELNRPLAEAAGVNIFQLAQTLTKA
                     NFNLASGNIRDPEGKFVIRSLATYQTIEQLEDTIVGQKNLRLKDIAEVVYDYPESDRI
                     DRYNGRPSMVLFVLKESQANTVEVCERIRAAVEEAQATPGMAGFQVEDIFNQGDAIVY
                     SLNQVTSSGLQGGVLAILVLLFFLRRVRLTLLIAASIPLSIFLALPFMYFAGQSINLI
                     SLLGLMICVGLVVDNSVVVAENIEHYRGRGMRPYAAALHGASEVALPITLATLTTMIV
                     FAPAALLSSGPTQFFMIRMVTPVCVSLLASLFVALALVPMASAVAFRDATPYLASKRW
                     LRPVLALDMTWKRWTTAAYEATMGPINRMYGRLLRVTLRRRMDVVVPSLLMFVATVAV
                     PMMQVPFSGGENMGTRNFFAYYSMPGDVTRAEADTFFRELEQTVEANKQEYRIAGQYI
                     GFDGTFAQVQVFFEKQTPGERPFKEVRQEVYDKLPNRPGWVKEARFGGSDGTQDDAFM
                     VTLYGDDHQSVQDTKEDLEQALLALPGVLGISSRGGDSLRRDELALSIDRSMSERFKV
                     SAGTIANTVAYAIRGQLLPRFQSPSEDREINVRIRYRKEDREQLSQLLEFKVLSDSGK
                     VVPINVLTESGLRKGENSLVRNDKRVAALIRLELAKEDRPETEKGIKAFLERYELPSG
                     LSFDADRNTREADDLATDLVGAMILST"
     gene            complement(2653..6288)
                     /locus_tag="VGB85_08610"
     CDS             complement(2653..6288)
                     /locus_tag="VGB85_08610"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0134128.1"
                     /translation="MDTQTPTAQRPEADEAQTLAWAARTKAMESYRFTTTRPVAVLMV
                     FCAVIVFGIFSIRLLPLNLMPDISYPRLTVRTEYPGAAPAEVENNVSRPMEEILGVVT
                     GLTKISSASRGGYSDVVLEFAWDTDMDEANQDVLEKLDAIKPVLPTEVKQPLILRYDP
                     TLDPVLTLSLSGSGEGFAGIDGLKYLRRIADRDVRRLLEPIEGVAAVKIKGGLEEEIQ
                     VQLLEEQLRRTNISVDTVIKRLQAENINLAGGSMRDGRTRYLVRTVNEFRDLADIREL
                     VILSRDGRDIKLKDIAEIGSGYKDRDVITRVDGREAVEVEVYKEADANIVDMANRVTL
                     ASLNTVRPRLKSEYGADIDVLTDRSLFIESSIDEVRDTAISGGLLAIIILFLFLRDLK
                     STLIVAISIPISVLVTFAPLNMAGVSLNIMSLGGLALGIGMLVDNSIVVLESIHRCKE
                     EGDDYVTATVRGTSEVGSAVISSTLTTVAVFFPMVFVEGIAGQMFGDLGLTVVFSLLA
                     SLAVALFLIPMLASRSWIGGPGLAAGEAPAVRLGRALGGLLTRWASAAELRASLGRPR
                     WWWVTIVPLVYYLARFVLHFAFELIGKLLSAILIVLVTVVVTVVGGALWLLGWVIKPV
                     LWLFDAFMRLLEAGYPRLIRWSLRNRVIVYGLVLAGGAATVWAAPQLDNELIPELHQG
                     EFTVELSLPVGTPLMATQETVLPVEQELLHAIPRLRSLITTIGSERDSSESGERGEHT
                     TRLRFALTTQKTRKDTIAKQAVLEEGEAVPEPEPIGLAAPPTDPNNAERDALAAVRPL
                     VQRIPDAIINVTRPALFSFTRPIEVEIRGYELDELGDATEAVRDTLAGIPGLRDVKSS
                     ILPGSPEVQIVYNRDALARYNLDIRTVAELVRNKVQGFEATKYNRKDRKIPVRVRLKD
                     IETADVEELRELVVNPGQARPVPLTAVAELTLGRGPNEIRRIGQQRVGLVTANLEGAG
                     LGSVVSEIESRLARVELPAGTSFAVTGQSAEWETSSRSLYLALGLSIFLVYVIMASQF
                     ESLIYPLIILVTIPLAAIGVIWTLLLLGLPVSVLVFLGAILLAGIVVNNAIVLVDYAG
                     QLKERGHTTDEALAIAGQVRLRPILMTTLTTVLGLIPMALGLGDGAELRTPMAITVIA
                     GLGFSTLLTLVVIPTLYAGVDRLFGGAVAEPRDVALAREAAAVTAAQLAPEAEVVAAQ
                     APEGRQE"
     gene            complement(6292..7509)
                     /locus_tag="VGB85_08615"
     CDS             complement(6292..7509)
                     /locus_tag="VGB85_08615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0134129.1"
                     /translation="MPVLFKSSSFALRPGRRGSLALVLMLSASSLAAAGCQANAGGVD
                     KKKDGKEEVAEVPTPVVVGAVERGAIVATISAASTIEAERQVTIHAESTGRLVDLTVE
                     EGDTVKKGQILARIRFDAQTNSLLRANTSLEKARVDFERAEKLFNDKVIGQEEFLRAQ
                     NALEIAQLDLSDRSREMRNTKVTAPFDGTITQRKVTAGAFVNNGAELLTIVDFRTLVA
                     RVFVPEKQLDRIQVGQEAEVVGKAARGRKGAGKILRIAPTIDASTGTVKVTVSMPPAL
                     AGPQAFLPGMYAEVTLRTDSRSDVPLVPKSAVVRDEEQTYLFTVTGDPTTGFIAKRVR
                     VETGLTDPERIEIVKGLAAGEPVITAGQTGLKDGAKVVRVDPQGKPLDPPIIAPISPA
                     LEVPAEANADKQE"
BASE COUNT         1147 a         2648 c         2544 g         1183 t
ORIGIN      
        1 tcgtcgaaag gatcatcgcg ccgacgaggt cggtcgccag gtcgtcggcc tcgcgggtgt
       61 tgcggtcggc gtcgaagctg aggccgctcg gcagctcgta gcgctcgagg aaggccttga
      121 tgcccttctc ggtctcgggg cggtcctcct tggccagctc gaggcggatc agcgcggcga
      181 cccgcttgtc gttgcgcacc agcgagttct cgcccttgcg caggccggac tcggtcagca
      241 cgttgatcgg cacgactttc ccgctgtcgc tgagcacctt gaattcgagc agctgcgaca
      301 gctgctcgcg gtcctccttg cggtagcgga tccggacgtt gatctcgcgg tcctcggacg
      361 ggctctggaa gcgcggcagc agctggccgc ggatcgcgta cgcgaccgtg ttggcgatcg
      421 tgccggccga caccttgaag cgctcgctca tcgagcggtc gatcgacagc gccagctcgt
      481 cgcggcgcag cgaatcgccg ccgcggctcg agatccccag cacccccggc agggccagga
      541 gcgcctgctc gaggtcctcc ttggtgtcct gcaccgactg gtggtcgtcg ccgtacagcg
      601 tcaccatgaa cgcgtcgtcc tgcgtgccgt cggagccgcc gaagcgggcc tccttcaccc
      661 agccgggccg attgggcagc ttgtcgtaga cctcctggcg gacctccttg aacggtcgct
      721 cgcccggcgt ctgcttctcg aagaacacct gcacctgcgc gaaggtcccg tcgaagccga
      781 tgtactggcc cgcgatccgg tactcctgct tgttcgcctc gaccgtctgc tcgagctcgc
      841 gaaagaacgt gtccgcctcg gccctcgtga cgtcgcccgg catcgagtaa taggcgaaga
      901 agttgcgggt gcccatgttc tcaccgccgg agaacggcac ctgcatcatc ggcaccgcga
      961 ccgtcgccac gaacatcagc agcgagggca ccaccacgtc catgcgccgg cgcagcgtca
     1021 cgcgcagcag ccggccgtac atgcggttga tcgggcccat cgtcgcctcg taggcggccg
     1081 tcgtccagcg cttccacgtc atgtcgagcg cgagcaccgg acgcagccag cgcttgctcg
     1141 ccagatacgg cgtcgcgtcg cggaacgcga ccgcgctggc catcggcacc agcgcgagcg
     1201 cgacgaacag gctggcgagc agcgacacgc acaccggcgt gaccatgcgg atcatgaaga
     1261 actgggtcgg cccggacgac agcagcgccg ccggggcgaa cacgatcatc gtcgtcagcg
     1321 tcgccagcgt gatcggcagc gcgacctcgc tcgcgccgtg cagcgccgcc gcgtagggcc
     1381 gcatgccgcg gccgcggtag tgctcgatgt tctcggcgac caccaccgag ttgtcgacca
     1441 ccagcccgac gcagatcatc agcccgagca gcgagatcag gttgatcgac tgccccgcga
     1501 agtacatgaa cggcagcgcg aggaagatcg acagcgggat cgaggcggcg atcaacagcg
     1561 tcagtcgcac ccgccgcaaa aagaacagca gcaccaggat cgccagcacg ccgccctgca
     1621 ggcccgagct ggtcacctgg ttgaggctgt agacgatcgc gtcgccctga ttgaagatgt
     1681 cctcgacctg gaagcccgcc atgcccggcg tggcctgggc ctcctcgacc gcggcccgga
     1741 tccgctcgca cacctcgacc gtgttcgcct gcgactcctt gagcacgaac agcaccatcg
     1801 acggccggcc gttgtagcgg tcgatgcggt cgctctccgg gtagtcgtag acgacctcgg
     1861 cgatgtcctt gaggcgcagg ttcttttgcc cgacgatcgt gtcctcgagc tgctcgatcg
     1921 tctggtaggt cgccagcgag cggatgacga acttgccctc ggggtcgcgg atgttgccgc
     1981 tggcgaggtt gaagttggcc ttggtcagcg tctgcgcgag ctggaagatg ttgacgcccg
     2041 ccgcctccgc gagcggccgg ttgagctcga tgcggatctc gcggtcctcc tggccccaca
     2101 ggttcaccag gccgacgccg tcgatgcgct cgatcgcccg caccatgtgc ttttgcaggc
     2161 ggtcacgcgg cagctcgacg gtctcgtccc acgtgatccc ataaaacgcg accgggatgc
     2221 ccgcgcccga ctccttgcgg atccggacct tctcgacgtc ggccggcaag tccggccgcg
     2281 cgcgcgcgac ccggtcgcgg acctcgcggt acgcgacgtt catgtcggtg tcgctctcga
     2341 acaccagcga caggctggcc cggtccgcgc gcgacgtggc gctcagctcg tcgaggcccg
     2401 gcgtcgtcga cagcgcctgc tcgagcggcc gcgtgatccg gtcctcgacg tcctgcgccg
     2461 tcgcgttggc gtacggcacc tcgacctgca tgaacggcgg cgagaagccc gaggggatca
     2521 gctcgatcgg gatgttcacc agcgcgacga tcccgatcac gataaagctg agcagcacca
     2581 tcaacatcgt caccgggcgg accagggaca gatgcacgag gctgttgtcg cggagcttgt
     2641 cgttggccat ggctactcct ggcgaccctc gggcgcctgc gcggcgacga cctccgcctc
     2701 gggcgcgagc tgggccgccg tcaccgccgc ggcctcgcgc gcgagcgcca cgtcgcgcgg
     2761 ctcggcgacc gcgccaccga acaggcggtc gacgccggcg tacagcgtcg ggatgacgac
     2821 cagcgtcagc agcgtcgaga agccgaggcc ggcgatgacg gtgatcgcca tcggcgtgcg
     2881 cagctcggcg ccgtcgccga ggccgagcgc catcgggatc aggccgagca cggtcgtcag
     2941 ggtcgtcatc aggatcgggc gcaggcggac ctggccggcg atcgccagcg cctcgtcggt
     3001 ggtgtggccg cgctccttga gctgaccggc gtagtcgacg agcacgatcg cgttgttgac
     3061 gacgatgccc gccagcaaga tcgccccgag gaacaccagc acgctgaccg gcaggccgag
     3121 cagcagcagc gtccagatca cgccgatcgc cgcgagcggg atcgtcacga ggatgatcag
     3181 cgggtagatc aggctctcga actgcgaggc catgatcacg tagaccagga agatcgacag
     3241 gccgagcgcc aggtacagcg agcgcgacga ggtctcccac tcggcgcttt gacccgtcac
     3301 cgcgaagctc gtgcccgccg gcagctcgac gcgcgccagc cgggactcga tctcgctgac
     3361 gacgctgccg aggcccgcgc cctcgaggtt ggcggtgacg aggccgacgc gctgctgacc
     3421 aatgcgccgg atctcgttcg ggccgcggcc gagcgtcagc tcggcgaccg ccgtcaacgg
     3481 caccgggcgg gcctggccgg gattgacgac cagctcgcgc agctcctcga cgtcggcggt
     3541 ctcgatgtcc ttgagccgca cgcgcaccgg gatcttgcgg tccttgcggt tgtacttggt
     3601 cgcctcgaag ccctgcacct tgttgcgcac cagctcggcg acggtgcgaa tgtcgaggtt
     3661 gtagcgggcc agcgcgtcgc ggttgtagac gatctggacc tcggggctgc cgggcaggat
     3721 cgacgacttg acgtcgcgca ggcccgggat gcccgccagc gtgtcgcgca cggcctcggt
     3781 cgcgtcgccg agctcgtcga gctcgtagcc gcggatctcg acctcgatcg ggcgcgtgaa
     3841 cgagaacagc gccggccgcg tgacgttgat gatcgcgtcc gggatccgct ggaccagcgg
     3901 gcgcaccgcg gcgagcgcgt cgcgctccgc gttgttcggg tcggtcggtg gcgccgccag
     3961 cccgatcggc tcgggctcgg gcaccgcctc gccctcctcg agcacggcct gcttcgcgat
     4021 cgtgtccttg cgggtctttt gcgtcgtcag ggcgaagcgc aggcgcgtgg tgtgctcgcc
     4081 gcgctcgccg gactcgctgc tgtcgcgctc gctgccgatc gtcgtgatca ggctgcgcag
     4141 gcgggggatc gcgtgcagca gctcctgctc gaccggcagc accgtctcct gcgtcgccat
     4201 caacggcgtg ccgaccggca ggctgagctc gacggtgaac tcgccctgat gcagctcggg
     4261 gatcagctcg ttgtcgagct gcggcgccgc ccacacggtc gccgcgccgc cggcgagcac
     4321 gagcccgtag acgatcacgc ggttgcgcag gctccagcgg atcagccggg gatagcccgc
     4381 ctcgagcagg cgcatgaacg cgtcgaacag ccacagcacc ggcttgatca cccagccgag
     4441 cagccacagc gccccgccga cgaccgtcac caccacggtg acgagcacga tcaggatcgc
     4501 cgacagcagc ttgccgatca gctcgaaggc gaagtgcagc acgaagcgcg cgaggtagta
     4561 gacgagcggg acgatcgtca cccaccacca gcgcgggcgc cccagcgacg ctcgcagctc
     4621 cgcggcggag gcccagcggg tcagcaggcc gcccagcgcg cgaccgaggc gcaccgcggg
     4681 ggcctcgccg gcggcgagcc cgggcccacc gatccagctg cgcgaggcga gcatcgggat
     4741 caggaacagc gccacggcca gggaggcgag caggctgaac acgaccgtca ggccgaggtc
     4801 gccgaacatc tgcccggcga taccctcgac gaagaccatc gggaagaaca ccgcgaccgt
     4861 cgtcagcgtc gacgagatca ccgcgctgcc gacctccgag gtcccgcgca ccgtcgccgt
     4921 gacgtagtcg tcgccctcct ccttgcagcg gtggatcgac tcgagcacga cgatcgagtt
     4981 gtcgaccagc atgccgatgc cgagcgccag cccgccgagg ctcatgatgt tgagcgagac
     5041 gcccgccatg ttgagcggcg cgaaggtcac cagcaccgag atcgggatcg agatcgcgac
     5101 gatcagcgtc gacttgaggt cgcgcaggaa caaaaacagg atgatgatcg ccagcaggcc
     5161 gcccgagatc gccgtgtcgc ggacctcgtc gatgctcgac tcgatgaaca gcgagcggtc
     5221 ggtcagcacg tcgatgtcgg cgccgtactc gctcttgagg cgcggccgaa ccgtgttcaa
     5281 cgaggccagc gtgacccggt tggccatgtc gacgatgttg gcgtccgcct ccttgtagac
     5341 ctcgacctcg accgcctcgc ggccgtcgac gcgggtgatc acgtcgcggt ccttgtagcc
     5401 cgagccgatc tccgcgatgt ccttgagctt gatgtcgcga ccgtcgcgcg acaagatcac
     5461 gagctcgcgg atgtcggcga ggtcgcgaaa ctcgttgact gtgcgcacga ggtagcgcgt
     5521 ccggccgtcg cgcatgctgc cgccggcgag gttgatgttc tcggcctgca ggcgcttgat
     5581 caccgtgtcg acgctgatgt tcgtgcgccg aagctgctcc tcgagcagct ggacctggat
     5641 ctcctcctcg aggccgccct tgatcttgac cgccgcgacg ccttcgatcg gctcgagcag
     5701 gcggcggacg tcgcggtcgg cgatcctgcg caggtacttg agcccgtcga tgccggcgaa
     5761 gccctcgccg ctgccgctga ggctcagcgt cagcaccggg tcgagcgtcg ggtcgtagcg
     5821 caagatcagc ggctgcttga cctccgtcgg cagcaccggc ttgatcgcgt cgagcttctc
     5881 caggacgtcc tggttggcct cgtccatgtc cgtgtcccac gcgaactcga gcacgacgtc
     5941 gctgtaacca ccgcgcgacg cgctcgagat cttcgtcagg cccgtgacga cgccgaggat
     6001 ctcctccatc ggcctggaaa cattgttctc gacctctgcc ggggccgcgc cggggtactc
     6061 ggtgcggacg gtcaaccgcg ggtacgagat gtccggcatc agattcaagg gcaacagccg
     6121 gatgctgaag atgccgaaca cgatcaccgc gcagaacacc atcagcaccg cgaccgggcg
     6181 cgtggtggtg aagcgatagc tctccatcgc cttcgtgcgg gcggcccacg ccagcgtctg
     6241 cgcctcgtct gcctccgggc gctgggccgt cggggtctgc gtgtccatgc gctactcctg
     6301 cttgtccgcg ttcgcctcgg ccgggacctc gagcgccgga ctgatcggcg cgatgatcgg
     6361 tgggtcgagc ggcttgccct gcgggtcgac gcggacgacc ttggcgccgt ccttgagccc
     6421 tgtctggccc gcggtgatca ccggctcgcc ggcggcgagg cccttgacga tctcgatccg
     6481 ctccgggtcc gtcaggccgg tctcgacgcg cacgcgcttg gcgatgaagc cggtcgtcgg
     6541 gtcgccggtg accgtgaaca ggtaggtctg ctcctcgtcg cggaccaccg ccgacttcgg
     6601 caccagcggg acatccgagc ggctatcggt gcgcagcgtc acctcggcgt acatgcccgg
     6661 caaaaacgcc tgcgggcccg cgagcgccgg tggcatcgag accgtcacct tgacggtgcc
     6721 ggtgctcgcg tcgatcgtcg gggcgatccg caggatcttg cctgccccct tgcgaccgcg
     6781 cgccgccttg ccgacgacct cggcctcctg cccgacctgg atgcggtcga gctgcttctc
     6841 cggcacgaac acgcgggcga cgagcgtacg aaagtcgacg atcgtcagca gttcggcgcc
     6901 gttgttgacg aacgcgccgg cggtcacctt gcgctgggtg atcgtgccgt cgaagggcgc
     6961 ggtcaccttg gtgtttcgca tctcgcgcga gcggtcgctg aggtcgagct gggcgatctc
     7021 cagcgcgttt tgggcgcgca ggaattcctc ctggccgatc accttgtcgt tgaacagctt
     7081 ctcggcccgc tcgaagtcga cgcgcgcctt ctccagcgag gtgttggcgc gcagcagcga
     7141 gttggtctgc gcgtcgaagc ggatccgggc caggatctgg cccttcttca cggtgtcgcc
     7201 ctcctcgacc gtcaggtcga cgagccgccc cgtcgactcc gcgtggatcg tcacctggcg
     7261 ctccgcctcg atcgtgctcg ccgccgagat cgtcgccacg atcgcgccgc gctcgacggc
     7321 gccgacgacc accggcgtcg gcacctccgc gacctcctcc ttgccgtcct tcttcttgtc
     7381 gacgccgccg gcgttcgcct ggcaaccggc cgccgccagc gacgacgccg acaacatcag
     7441 gaccagcgcc agcgaccccc gacgaccggg acgcagcgcg aacgacgacg acttgaaaag
     7501 cacgggcatg gcgagggctc ag
//
LOCUS       DASWNJ010000509         1954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_468171, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000509 DASWNJ010000000
VERSION     DASWNJ010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468171"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            567..1706
                     /locus_tag="VGB85_08620"
     CDS             567..1706
                     /locus_tag="VGB85_08620"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEY0134130.1"
                     /translation="MRITGLRSFMTRDRDRPRVVVAIDTDAGVTGWGECYNHGPDRAL
                     PPLLDYLYLQIEGRDPRRIEFLVLKLLQQSRFPPGALGLAAISAIDHCLWDISAKALG
                     VPVYMLLGGHARDRVRVYLGVYTAPGPEALKDACLGHEERYGLTAFKLSPYRGDLHAL
                     PWGEVVKGSADWFARIRETCPSRFEFAFDAHAKLFEPWQAVQLGNALAPLHPLFFEEP
                     IRPENIEAWGRLKAQLHCPLATGESLYSRFEFLRLLQVAGADIIQPDICVVGGLLEMR
                     KIAALAEAHYVTVAPHNPMGPLATAVNLHFCAAQPNFKILEYRLPEQAPYVKDPYLPK
                     DGHLELRPDRPGWGVEIDEAVLARDGYVHWERKLPVRPDGSTGYV"
     gene            1721..>1954
                     /locus_tag="VGB85_08625"
     CDS             1721..>1954
                     /locus_tag="VGB85_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134131.1"
                     /translation="MPRITAARVTPVAFRDPPLLNSAGVHEPWALRSIVEVEVEGEVT
                     GLGETYGDEPFLTLLRRCADAVVGLDVHDLNGVK"
BASE COUNT          273 a          724 c          679 g          278 t
ORIGIN      
        1 ggtggtgcgg ttgcggcgct gggacttctc gaccccgacc ggcgtccgcg agggcttcga
       61 cgccgccgac cggaggggca ggccggggcg gccgaccccg gccgaggtcg aggccagcgt
      121 cgccgcgacg atctactcgg cctggcgggg ccggatgctg cggaacacga tcgacgcgac
      181 gctcgacgac ctgggcgtgc ccgagccggt gacgcaggac cagtacgtga ccgcgctgcg
      241 ccgcctgcta gaggagttcc ctcagaacag gggcgtgggc gagtcgggcg tgaacttctt
      301 ctccctcgat cgggtcgaga acgagtgccg ccgcgccggg accgcggcct cgccggcgct
      361 cgcccccgag gcgcgccggg acgtcgtcgt cctctcaagc ctcgcacagg cgctcgaccg
      421 gctcgccggg gaggagttcg aggacgcctt ccggaggtcg accagtcagg cggactaccg
      481 ctggggcagg ctgcaccggg tcgtcttccc ccacccccct gggcgagcgg ttctcggtca
      541 agagcacgca tcgaggagag aacgccttgc gcatcaccgg gcttcgcagc ttcatgaccc
      601 gcgaccgcga ccggccgcgg gtcgtcgtcg ccatcgacac ggacgcgggc gtcaccggct
      661 ggggcgagtg ctacaaccac gggcccgacc gcgcgttgcc gccgctcctc gactatctct
      721 acttgcagat cgagggccgc gacccgcgcc ggatcgagtt cctggtcctg aagctgctgc
      781 agcagagccg cttcccgccg ggcgccctgg gcctcgccgc catctccgcc atcgaccact
      841 gcctgtggga catctcggcc aaggctctcg gcgtgccggt ctacatgttg ctgggcgggc
      901 acgcgcgcga ccgggtgcgg gtctacctcg gcgtctacac cgccccgggt cccgaggcgc
      961 tcaaggacgc ctgcctcggg cacgaggagc gctacgggct caccgccttc aagctgagcc
     1021 cctaccgcgg cgacctgcac gccctgccgt ggggcgaggt ggtgaagggc agcgccgact
     1081 ggttcgcgcg tatccgggaa acatgcccga gccggttcga gttcgccttc gacgcgcacg
     1141 ccaagctctt cgagccctgg caggcggtgc agctcggcaa cgcgctggcg ccgctccacc
     1201 cgctcttctt cgaggagccg atccggcccg agaacatcga ggcctgggga aggctgaagg
     1261 cccagctcca ctgcccgctc gccaccggcg agagcctcta cagccgcttc gagttcctgc
     1321 gcctcctcca ggtcgcgggc gccgacatca tccagcccga catctgcgtg gtcggcgggc
     1381 tgctcgagat gcgcaagatc gcggccctcg ccgaggcgca ctacgtcacg gtggcgccgc
     1441 acaacccgat gggccccttg gccacggcgg tgaacctgca cttctgcgcc gcccagccca
     1501 acttcaagat cctggagtat cgcctccccg agcaggcccc ctacgtgaag gatccctacc
     1561 tgcccaagga cggccacctc gagctgcggc ccgaccggcc gggctggggg gtcgagatcg
     1621 acgaggcggt gctggcgcgt gacgggtacg tccattggga gcgcaagctg ccggtccgtc
     1681 ccgacggttc gacgggctac gtctgaggag ggagcgagcc gtgccccgca tcaccgccgc
     1741 gcgcgtcacg ccggtcgcct tccgcgaccc gccgctgctc aactcggccg gcgtccacga
     1801 gccctgggcg ctgcgcagca tcgtcgaggt ggaggtcgag ggcgaggtca cgggcctggg
     1861 cgagacctac ggcgacgagc ccttcctcac cctcctgcgc cgctgcgccg atgcggtcgt
     1921 cgggctcgac gtccacgacc tcaacggggt caag
//
LOCUS       DASWNJ010000510         4026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_679229, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000510 DASWNJ010000000
VERSION     DASWNJ010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679229"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1492)
                     /gene="hxsB"
                     /locus_tag="VGB85_08630"
     CDS             complement(<1..1492)
                     /gene="hxsB"
                     /locus_tag="VGB85_08630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His-Xaa-Ser system radical SAM maturase HxsB"
                     /protein_id="HEY0134132.1"
                     /translation="MSMQTLDLATIRPRAGTQAFFRHREVGDRVLVTNFEGNHLLLTR
                     EEFALFARGEVPDASDLHTRLTAGNFLRASYDVRKAAAALQARKRFLHAGPNLHIFVV
                     TLRCNETCVYCHASRANMDQVQTDMTPEIAEKAVELALRSTSPFITIEFQGGEPLVNF
                     PVVQHICEYAGRRAQELGKQLQFTMVSNFALMDDTKLDYLVAHRVQLCTSIDGPEALH
                     NAQRKLPTMDAHQRAVHWIKRINQRYIDMGLDPTLYHVEALLTTTRETLPRWKEVVDT
                     YVALGCKALFLRPIDPFGFADRTRLRLEYPRSAYLEFYRNAVDYMLELNRQGVQILER
                     YAGIFLTKIMRGEDPNFLDLRSPAGAGIGQLAYNYDGKIFSCDEGRMLHEMGDSKFHL
                     GDVHTSSYRDIVGHETVRAMVIASNLDGQPDCVNCAYQPYCGTISTFNYKHHGSIFGR
                     MRESSVCAVHKGIQDYLFEKLAAGDPHTLRSFDRWTTNRPREHFVQA"
     gene            complement(1497..1727)
                     /locus_tag="VGB85_08635"
     CDS             complement(1497..1727)
                     /locus_tag="VGB85_08635"
                     /inference="COORDINATES: protein motif:HMM:NF038315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HxsD-like protein"
                     /protein_id="HEY0134133.1"
                     /translation="MTEPAFLDLRFHRTLYAGPAVDEAIKVFARHGEFHMSEETDSWV
                     VRLRALRPALTRRLAHELANYALGLTVQRGGV"
     gene            complement(1724..2230)
                     /gene="hxsD"
                     /locus_tag="VGB85_08640"
     CDS             complement(1724..2230)
                     /gene="hxsD"
                     /locus_tag="VGB85_08640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His-Xaa-Ser system protein HxsD"
                     /protein_id="HEY0134134.1"
                     /translation="MQSSYPDIPPGLVAADLGEAAVQFHVDPAIYPVQAVYGAAYIFI
                     DRCYVFLDRPQSSEPAIVRVTLTAKGGQADAGALRALVGEFANELLSCAWRHQITQDN
                     RVLLETVTMQAIAGAMGPPSLDDLASFDFSDQGFDDPLGIAMSWEEKHGKKQPSASST
                     DEAGETAP"
     gene            2328..>4026
                     /locus_tag="VGB85_08645"
     CDS             2328..>4026
                     /locus_tag="VGB85_08645"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HEY0134135.1"
                     /translation="MHWTALRTVGLLLWVLPIAACPGSDGVAETGGTHGEGSSEGPGA
                     PPTSTTGTTGPAATTGTSLPPDPTTADATAAPTAGHTTGDPPPDLPPAIQCDPGAGGP
                     YWLLEGESVEFTVACKTGLELDGGAFTIDPLPAGAKYDAATATLTWTPGLDQGAVYNL
                     TITADPFGEAGGVKIGVADRWDDPANVPVVDPLVYGKEYGLPVLHLQADDAINADAYT
                     PATVIYAGHTYTAEAKYRGAASLGYPKKSYTLKFSKDDKFNEPGRAGGFLEKRKVVLI
                     STFDDNAYVRQRLAYELWNSLDPEHVQIQVYSGVVFLDGEYYGLYTFADHVDGYLMED
                     HGLAQDGNLYKSRTHDANFRLTKSGGNDSKSTPHEGLTKEEGVPLDGEPGAYDDLDEL
                     ITFVATAKDAAFLAEIDDRIDRRDYEDWWIFVSLIMGDDSAGKNAYHYHDPAMGPWRY
                     MPWDFNDSFGQTWQTARKGFDAQPDGYAWANELFARFLELPAIGDPLRARYGEALQGV
                     YQIDAVLERLDGMLDETADSALRDEGRWATQYQTYGGWNWRDDFTNYDEEVEYLRQWI
                     "
BASE COUNT          641 a         1283 c         1471 g          631 t
ORIGIN      
        1 cggcctggac gaagtgctcg cgcggccggt tcgtcgtcca gcgatcgaag ctgcgcagcg
       61 tgtgcggatc gccggcggcc agcttctcga acaggtagtc ctggatgccc ttgtggaccg
      121 cgcagacgct gctctcgcgc atgcggccga agatgctgcc gtggtgcttg tagttgaacg
      181 tcgagatcgt gccgcagtag ggctggtagg cacagttgac gcagtcaggc tggccgtcga
      241 ggttggacgc gatcaccatc gcgcggacgg tctcgtggcc gacgatgtcg cggtagctgc
      301 tggtgtgcac gtcgccgagg tggaacttgc tgtcgcccat ctcgtgcaac atcctgccct
      361 cgtcgcagct aaagatcttg ccgtcatagt tgtaggcgag ctggccgatg ccggcgccgg
      421 cggggctgcg caggtcgagg aagttggggt cctcgccgcg catgatcttc gtcaagaaga
      481 tgccggcgta gcgctcgagg atctgcacgc cctggcgatt gagctcgagc atgtagtcga
      541 ccgcgttgcg gtagaactcg aggtacgcgc tgcgtggata ttccaggcgc aggcgggtgc
      601 ggtcggcgaa gccgaagggg tcgatcgggc gcagaaacag cgccttgcag ccgagggcga
      661 cgtaggtgtc gacgacctcc ttccagcgcg gcagcgtctc gcgggtggtc gtgagcagcg
      721 cctcgacgtg gtagagcgtc gggtcgaggc ccatgtcgat gtagcgctgg ttgatccgct
      781 tgatccagtg gaccgcgcgc tggtgggcgt ccatcgtcgg cagcttgcgc tgggcgttgt
      841 gcagggcctc ggggccgtcg atgctggtgc acagctgcac gcggtgggcg acgaggtagt
      901 cgagcttggt gtcgtccatc agcgcgaagt tcgacaccat cgtgaactga agctgcttgc
      961 cgagctcctg cgcgcggcgg cccgcgtact cgcagatgtg ctggacgacc gggaagttga
     1021 cgagcggctc gccgccctgg aactcgatcg tgataaacgg gctcgtgctg cgcagggcca
     1081 gctcgaccgc cttctcggcg atctccggcg tcatgtcggt ctggacctgg tccatgttgg
     1141 cgcgcgaggc gtggcagtac acgcaggtct cgttgcagcg cagcgtgacg acgaagatgt
     1201 gcaggttcgg gccggcgtgc aggaagcgct tgcgggcctg cagcgccgcg gcggccttgc
     1261 gcacgtcgta gctggcgcgc agaaaattac cggcggtgag gcgcgtgtgc aggtcgctgg
     1321 cgtcggggac ctcgccgcgg gcaaatagcg cgaactcctc gcgcgtcagc agcaggtggt
     1381 tgccctcgaa gttggtgacg agcacgcggt cgccgacctc gcggtggcga aagaacgcct
     1441 gggtgccggc gcgtgggcgg atcgtcgcga ggtcgagtgt ttgcatgctc atatactcag
     1501 acgcctccgc gctggacggt caggccgagg gcgtagttgg cgagctcgtg cgcgaggcgg
     1561 cgggtcagcg cgggccgcag cgcgcgcagg cggaccaccc agctgtctgt ctcctcggac
     1621 atgtgaaact cgccgtggcg ggcgaacacc ttgatcgcct cgtcgaccgc ggggccggcg
     1681 tagagcgtgc gatgaaagcg gaggtcgagg aacgcgggct cggtcacggg gcggtctccc
     1741 cggcctcgtc cgtgctggac gccgaaggtt gcttcttgcc gtgcttctct tcccacgaca
     1801 tcgcgatgcc gagcgggtcg tcgaagccct gatcggagaa gtcgaagctg gcgaggtcgt
     1861 cgagcgaggg cgggcccatc gcgccggcga tcgcctgcat cgtcacggtc tccaacagta
     1921 cgcggttgtc ctgcgtgatc tggtggcgcc acgcgcagct gagcagctcg ttggcgaact
     1981 cgccgaccag cgcgcgcagg gccccggcgt cggcctggcc gcccttggcg gtcagggtca
     2041 cgcgcacgat cgcgggctcg ctcgactgcg ggcgatcgag gaacacgtag cagcgatcga
     2101 tgaagatgta cgcggccccg tagaccgcct gcacgggata gatcgcgggg tcgacgtgga
     2161 actgcacggc ggcctcgccg aggtcggcgg ccaccaggcc cggggggata tcaggatacg
     2221 aggactgcat cgtcggccag gcgcgacctt atcaggtttg cgctcgccgc ggcgcggtgg
     2281 tgcgcgcccg ggtttccgct ttgccacggg agtgctaggt tggtgtgatg cactggaccg
     2341 cactgcgcac cgtgggcctg ctgttgtggg tgttgccgat cgccgcgtgc ccgggctccg
     2401 acggggtcgc ggagaccggc gggacgcatg gcgagggttc gagcgagggg ccgggcgcgc
     2461 cgccgacgag tacgaccggc acgaccggac cggcggcgac gaccggcacg tcgctgcccc
     2521 ccgatccgac gaccgcggac gcgacggcgg cgccgaccgc ggggcacacg accggcgatc
     2581 cgccgcccga cctgccgccc gcgatccagt gcgatcccgg ggcgggcggg ccgtactggc
     2641 tgctcgaggg cgagagcgtc gaattcacgg tcgcctgcaa gaccggcctc gagctcgacg
     2701 gtggggcctt cacgatcgac ccgctgccgg ccggcgcgaa gtacgacgcg gccaccgcga
     2761 cgctgacgtg gacgcccggc ctcgatcagg gcgccgtcta caacctcacg atcacggccg
     2821 acccgttcgg cgaggccggt ggcgtcaaga tcggcgtcgc cgatcgctgg gacgacccgg
     2881 ccaatgtccc ggtggtcgac ccgctggtgt acggcaagga gtacggcctg ccggtgctgc
     2941 acctgcaggc cgacgatgcg atcaacgcgg acgcgtacac gccggcgacg gtgatctacg
     3001 ccgggcacac gtacacggcg gaggccaagt atcgcggggc ggcgtcgctc gggtacccga
     3061 agaagagcta cacgctcaag ttcagcaagg acgacaagtt caacgagccc gggcgcgcgg
     3121 gcggcttcct cgagaagcgc aaggtcgtgc tgatcagcac cttcgacgac aacgcctacg
     3181 tgcgccagcg cctcgcctac gagctgtgga acagcctcga ccccgagcat gtgcagatcc
     3241 aggtctactc cggcgtggtc ttcctcgacg gcgagtatta cgggctctac accttcgccg
     3301 accacgtcga cggctacctg atggaggacc acgggctcgc gcaggacggc aacctctaca
     3361 agtcgcgcac acacgacgcc aactttcgcc tgaccaagag cggcggcaac gactccaaga
     3421 gcacgccgca cgaggggctg acgaaggagg agggcgtgcc gctcgacggg gagcccggcg
     3481 cctacgacga cctcgacgag ctgatcacct tcgtggcgac cgccaaggac gcggcatttt
     3541 tggccgagat cgacgatcgc atcgaccgcc gcgattatga ggactggtgg atcttcgtca
     3601 gcctgatcat gggcgacgac tcggccggca agaacgccta ccactaccac gacccggcga
     3661 tggggccgtg gcgctacatg ccgtgggact tcaacgacag ctttggccag acctggcaga
     3721 ccgcgcgcaa gggcttcgac gcgcagcccg acggctacgc gtgggccaac gagctgttcg
     3781 cgcgcttcct cgagctgccg gcgatcggcg acccgctgcg ggcccgctac ggcgaggcgc
     3841 tgcagggggt ttatcagatc gacgcggtgc tcgagcgcct cgacgggatg ctcgacgaga
     3901 cggccgacag cgcgctgcgc gacgagggcc gctgggcgac gcagtatcag acgtatggcg
     3961 gctggaactg gcgcgacgac ttcaccaact acgacgagga ggtcgagtac ctgcgccagt
     4021 ggatca
//
LOCUS       DASWNJ010000511         3524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_324155, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000511 DASWNJ010000000
VERSION     DASWNJ010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324155"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /locus_tag="VGB85_08650"
     CDS             <1..1238
                     /locus_tag="VGB85_08650"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEY0134136.1"
                     /translation="SLFTMASAVCVTGLSVNDVPTTYTFFGQAVILAGIQLGGIGIMT
                     IAALALAFSRNTSLQSQLRYAAMLDARTLADLRQIVVGIIFGTFAIEACGALALWFLF
                     SGDPRIGDASPLWMAVFHSISAFCNAGFALFPGNLMGFVGDTGVQTVIMLLIVLGGLG
                     FPVIMELVRTGGRRALRVVRKSTPMPPRLSLTSRVVVTTSLLLIAVGTVAVLLLEFTN
                     ALAPAGEAMTGGRVLAALFASVNTRTSGFNTVDLVVLKDSTLMIMCVLMFIGGSPAST
                     AGGIKTTTAAVVFATLRAELRGREPELGGRAIAADALRKAIAVSAMSLAVVLTVILLL
                     TLTEDQKFIHLAFEAVSAFATVGLTAGVTASLSVAGKLIITATMFVGRVGPFTIALAV
                     GRDVEAQRYRLAREDLPVG"
     gene            1280..1735
                     /locus_tag="VGB85_08655"
     CDS             1280..1735
                     /locus_tag="VGB85_08655"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEY0134137.1"
                     /translation="MSSNQRYLVIGLGRFGAALAESLSEQGAEVIAVDNRMHEVEAVK
                     RKVAFALELDATDPLALQSVEPQRCTAVIVAIGENFEATVLTMAALREIGVKNIIARA
                     RSAREARILQAVGASEIIELETEMGRMFGRRLSSKAMDGDIAVLPGAKT"
     gene            complement(1881..2132)
                     /locus_tag="VGB85_08660"
     CDS             complement(1881..2132)
                     /locus_tag="VGB85_08660"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0134138.1"
                     /translation="MHIVLDTYNLTAVERLLCETALTHAGSIVEAAKLLGITRHALKR
                     RIIKHGIRWSRDGGRISAGANDGAGRVAAGASAVAAVSA"
     gene            complement(2328..2831)
                     /locus_tag="VGB85_08665"
     CDS             complement(2328..2831)
                     /locus_tag="VGB85_08665"
                     /inference="COORDINATES: protein motif:HMM:NF022161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HEY0134139.1"
                     /translation="MNYTISDEFDTSIERYWQVFFDDTYNAELFKRLKVGREVLEMKR
                     EGEGDSLVIRRKLRLSPEREVPALVKKFVKGAVTYTEENVFTARNNTIEVVTIPGFMA
                     DALTTRGTYRVQALGPNKVRRTWEGFIDCKIPLLGGKVEKHIVEEVTQSYRDTTDFTR
                     KWLAEHP"
     gene            3003..>3524
                     /locus_tag="VGB85_08670"
     CDS             3003..>3524
                     /locus_tag="VGB85_08670"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEY0134140.1"
                     /translation="MPGPAPQARPGDRVALQDGCVGCHPAVAREWAQSLHRMSASDEP
                     YREAFAREPLPFCRKCHVPEAMPKQPASPWAEEVGVGCVTCHVTTRHGVLATGTAQGS
                     PHARGTSAAMQAFAGPGACAGCHEFKFPGVDLGRPDDMQETLREHADSWARDYACADC
                     HMPWVDEGEGRGHR"
BASE COUNT          487 a         1137 c         1324 g          576 t
ORIGIN      
        1 actcgctgtt cacgatggcg agcgcggtgt gtgtgacggg cctgtcggtc aacgacgtgc
       61 cgacgaccta cacgttcttc gggcaggcgg tgatcctcgc ggggatccag ctcggcggca
      121 tcggcatcat gacgatcgcc gcgctcgcgc tggcgtttag ccgcaacacc tcgctgcaga
      181 gccagctgcg ctacgcggcg atgctcgacg cgcgcacgct cgcggacctg cggcagatcg
      241 tcgtcggcat catcttcggg acgtttgcga tcgaggcctg cggggcgctc gcgctgtggt
      301 tcctgttctc cggcgacccg cgcatcggcg acgcgtcgcc gctgtggatg gcggtgttcc
      361 actcgatctc ggcgttctgc aacgccggct tcgcgctgtt cccgggcaac ctgatgggct
      421 tcgtcggcga cacgggcgtg cagacggtga tcatgctgct gatcgtgctc ggcgggctcg
      481 gcttcccggt gatcatggag ctggtgcgca ccggcgggcg gcgagcgctg cgggtggtcc
      541 gcaagagcac gccgatgccg ccacgcctga gcctgacgag ccgcgtggtg gtgacgacca
      601 gcctgctgct gatcgcggtc gggacggtcg cggtgctgct gctcgagttc acgaacgcgc
      661 tcgcgcccgc gggcgaggcg atgaccggcg ggcgcgtgct cgcggccctg ttcgcctcgg
      721 tcaacacgcg cacctcgggg ttcaacaccg tcgacctggt ggtcctcaag gactcgacgc
      781 tgatgatcat gtgcgtgctg atgttcatcg gcggctcacc ggcgtcgacg gccggcggca
      841 tcaagaccac gaccgcggcg gtggtgttcg cgaccttgcg cgcggagctg cgcggacgcg
      901 agcccgaact cggcgggcgg gcgatcgccg ccgacgcctt gaggaaggcg atcgcggtca
      961 gcgcgatgtc cctggcggtc gtcttgacgg tgatcttgct gctcacgctg accgaggacc
     1021 agaagttcat ccacctcgcg ttcgaggcgg tgtccgcgtt cgccaccgtg ggtctcaccg
     1081 cgggggtcac cgcctcgctg agcgtggccg gtaagttgat catcacggcg acgatgttcg
     1141 tcggccgcgt cgggccgttt acgatcgccc tggcggtcgg gcgtgacgtc gaggcccagc
     1201 gctatcgcct cgcccgcgag gacctgccgg tcggctgatc gggcggtgac aagcggcgcg
     1261 gcgcttgcga agatgcggca tgtccagcaa ccagcgctac ctcgtcatcg gtctcgggcg
     1321 cttcggggcg gcgctcgccg agtcgctcag cgagcagggg gccgaggtga tcgccgtcga
     1381 taatcgcatg cacgaggtcg aggcggtcaa gcgcaaggtc gccttcgcgc tcgagctcga
     1441 cgcgaccgac cccctggccc tgcagtcggt cgagccgcag cggtgcaccg cggtgatcgt
     1501 ggcgatcggc gagaacttcg aggcgacggt gctgacgatg gcggcgctgc gcgagatcgg
     1561 ggtcaagaac atcatcgcgc gggcccgcag cgcgcgcgag gcgaggatcc ttcaggcggt
     1621 cggcgcctcg gagatcatcg agctcgagac cgagatgggc cgcatgttcg ggcgccggct
     1681 gtcgagcaag gcgatggacg gcgacatcgc ggtgctgccg ggcgcgaaga cctgaccgaa
     1741 gggacaggcc aaaacggcga agggccgcat cgacgttccg tcgaggcgac ctcctgagcc
     1801 gggctttatg gttatttttt aagaagaacc gcgtgatccc cgggcgacgc cgaggaggcg
     1861 cgagctcgcc aggcgcggcc tcaggccgag accgcggcga cggcggaggc accggcggcc
     1921 acgcggccgg cgccatcgtt cgcgcccgcc gagatccggc caccatcgcg gctccagcgg
     1981 atgccgtgct tgatgatgcg ccgcttgagg gcgtgacgcg tgatgccgag cagcttggcg
     2041 gcttcgacga tgctgccggc gtgggtgagg gcggtctcgc aaaggagacg ctcgacggcg
     2101 gtgaggttgt aggtgtcgag gacgatgtgc atgaagcgta tgtagaacac ccgcttgatc
     2161 catcggagac aaggcgctcg cccaatgaac aggccgtcgc ttgcgtcgga ggcgtcgagc
     2221 ggggccagta cgtccggcgt cgagtgacgt gcgcgctcgc gagcatcgtc tgcgcagcgc
     2281 gcttcacagg cacatcggcc gcgccgcgca atgtgccgtg ggggccctca ggggtgctct
     2341 gcgagccact tgcgggtgaa gtcggtggtg tcgcggtagc tctgcgtgac ctcctcgacg
     2401 atgtgcttct cgaccttgcc gccgagcagc gggatcttgc agtcgatgaa gccctcccag
     2461 gtccgccgca ccttgttggg gccgagagct tgcacgcggt aggtgccgcg ggtggtcagg
     2521 gcgtcggcca tgaagccggg gatcgtgacg acctcgatcg tgttgttgcg cgccgtgaac
     2581 acgttctcct cggtgtaggt gacggcgccc ttgacgaatt tcttgaccag cgcagggacc
     2641 tcgcgctcgg ggctcagccg gagcttgcgg cggatgacga ggctgtcgcc ctcaccctcg
     2701 cgcttcatct cgaggacctc gcggccgacc ttgaggcgct tgaacagctc ggcgttgtag
     2761 gtgtcgtcga agaagacctg ccagtagcgc tcgatgctgg tgtcgaattc gtcgctgatc
     2821 gtgtagttca tggcccggtc accttcgcgc gggcggcgag caggcgtcaa gcggtggggt
     2881 cgctttacag acggggcggt gggcggcact gtggggccat cctgcgggga cgcttcactc
     2941 tcgggctcag ttttgggctc acgctggcgt gcgtgggcgg gctggcgtgg gcgaggaccc
     3001 cgatgccagg gccggccccg caggcgcgcc ccggcgaccg cgtcgcgctg caggacggct
     3061 gcgtcggctg ccaccccgcg gtcgcccgcg agtgggcgca gtcgctgcac cgcatgtccg
     3121 ccagcgacga gccgtaccgc gaggcgttcg cgcgcgagcc gctgcccttc tgtcgcaagt
     3181 gccatgtccc ggaggccatg cccaaacagc cagcttcgcc gtgggcggag gaggtcgggg
     3241 tcggctgcgt gacctgccac gtgacgacgc gccacggggt gctggcgacc ggcacggcgc
     3301 agggcagccc gcacgcgcgg gggacgagcg cggcgatgca ggcgttcgcg gggccaggcg
     3361 cgtgcgcggg gtgccacgag ttcaagttcc cgggcgtcga cctcgggcgg cccgacgaca
     3421 tgcaggagac gctgcgcgag cacgcggact cgtgggcccg cgactacgcg tgcgccgact
     3481 gtcacatgcc ctgggtcgac gagggcgagg ggcgcgggca ccgg
//
LOCUS       DASWNJ010000512         1818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_503458, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000512 DASWNJ010000000
VERSION     DASWNJ010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503458"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1624
                     /locus_tag="VGB85_08675"
     CDS             <1..1624
                     /locus_tag="VGB85_08675"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0134141.1"
                     /translation="GTIVSERYRVISTLGAGGMGTVYLAEHLTVGRRVAIKVLNGEWS
                     GHKFVARRFQAEARIASTIGHPNIVEVFDAGQLPDKRLFLVMEHLDGHDLAQEIADRG
                     TLSPLRCCEILRQVALALAAAHNVNVIHRDLKPGNIMIANGAGGEVVKILDFGIACSP
                     RATAREGQRLTLPGSVMGTPEYMAPEQSTSAEPNPRFDLYALGAIAFEMLTGDPPMLA
                     EHAYELLVRKRKEASPSLGTRAPHLHPDLIALVDDCLEITPAKRPLDAIEFIHRLDAF
                     IDELPDDALPDDPFSAEPIAPGHNGTGSFSALNASAAASAAAAVSGAMAAQSANLTGP
                     RAAIPIRPPSTSRTPMPRMSAPPRPRPAWLPAAIVVAALVIGAWLLMSIFDLSSKRAL
                     TEVADDTAEPVAESTPERPLGLDRPQTPVTTLRKLPSTPPVIPTPEPTPEPTPQVTSK
                     PRPAEKREFNTPECERVRARADEARRTQAWGRLRDLSKKRTCWQTDAEARKLQTKALM
                     ELGDFTGCMSAGQGLKDKEVEQWRKLCERRAG"
BASE COUNT          260 a          758 c          557 g          243 t
ORIGIN      
        1 agggacgatc gtctccgaac gttatcgcgt catctcgacc ctcggcgccg gcggcatggg
       61 caccgtctac ctggccgagc acctgaccgt cggccgtcgg gtcgctatca aggtgctcaa
      121 cggcgagtgg agcggccaca agttcgtcgc ccgccggttc caggccgagg cccggatcgc
      181 cagcacgatc ggccacccca acatcgtcga ggtcttcgac gccggccagc tgcccgacaa
      241 gcgcctgttc ctggtgatgg agcacctcga cggccacgac ctcgcccagg agatcgccga
      301 ccgcggcacc ctctcgccgc tgcgctgctg cgagatcctt cgccaggtcg ccctcgccct
      361 cgccgccgcg cacaacgtca acgtcatcca ccgcgacctc aagcccggca acatcatgat
      421 cgccaacggg gccggcggcg aggtcgtcaa gatcctcgac tttggcatcg cgtgcagccc
      481 ccgcgccacc gcccgcgagg gccagcgcct gaccctcccc ggctcggtga tgggcacccc
      541 cgagtacatg gcgcccgagc agtcgaccag cgccgagccc aacccccgct tcgacctcta
      601 cgccctcggc gcgatcgcct tcgagatgct caccggcgac ccgccgatgc tcgccgagca
      661 cgcctacgag ctgctggtcc gcaagcgcaa ggaggcctcg ccctcgctcg gcacccgcgc
      721 gccgcacctc caccccgacc tcatcgcgct ggtcgacgac tgcctcgaga tcacccccgc
      781 caagcggcca ctcgacgcga tcgagttcat ccaccgactc gacgccttca tcgacgagct
      841 ccccgacgac gccctccccg acgacccttt ctccgcggag ccgatcgcgc ccgggcacaa
      901 cggcaccggc agcttctcgg ccctcaacgc atcggccgcc gcgtcggcgg ccgcggcagt
      961 gtccggcgcg atggctgcgc agtcggccaa cctcaccggc ccacgcgctg cgatcccgat
     1021 ccgtcccccg tcgacctcgc gcacgccgat gccgcgcatg tcggcgccgc cgcgcccgcg
     1081 tcccgcgtgg ttgccagccg cgatcgtcgt cgccgcgctc gtgatcggcg cgtggctgtt
     1141 gatgtcgatc ttcgacctgt cctccaagcg ggccctcacc gaggtcgccg acgacaccgc
     1201 cgagccggtc gccgagtcga ccccggagcg cccgctgggc ctcgatcgcc cgcagacccc
     1261 cgtcacgacc ctgcgcaagc tgccctcgac cccgccggtg atcccgaccc ccgagccgac
     1321 gccggagccg accccgcagg tgacgagcaa gccgcgtccg gccgagaagc gagagttcaa
     1381 cacccccgag tgcgagcgtg tgcgcgcccg cgccgacgag gcccgtcgta cgcaggcctg
     1441 gggccgtctc cgcgatctct cgaagaagcg cacctgctgg caaaccgacg ccgaggcgcg
     1501 caagctccag accaaggcgc tgatggagct cggtgacttc accgggtgca tgtccgcagg
     1561 ccagggtctc aaggacaagg aggtagaaca gtggcggaag ctatgcgagc ggcgcgccgg
     1621 ttaggcagcc cgggttcatc gggctggatc ctcgtcgccg cgctctggac cgtctccggt
     1681 ccggccgcgg ccgcgtctcc cacggcgtcg ccgtctccca cgtcgtcgcc cgcgcccaca
     1741 tcttcgcctg cgcccgcctc gtcgcccgcg cccgcctcgt cgcccgcgcc cgcctcgtcg
     1801 cagccggccc cggccgag
//
LOCUS       DASWNJ010000513         3076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_221109, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000513 DASWNJ010000000
VERSION     DASWNJ010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221109"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VGB85_08680"
     CDS             <1..196
                     /locus_tag="VGB85_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134142.1"
                     /translation="AQLDYDLNALPDVGGATQREIHAVFDLGSNTLSGDINGPAGTYT
                     LTGTPGTGNQARIVVDISQK"
     gene            complement(200..595)
                     /locus_tag="VGB85_08685"
     CDS             complement(200..595)
                     /locus_tag="VGB85_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY0134143.1"
                     /translation="MAEPSPTDADALLSAYLDKQLAPDEAARVEKLLAESPEAREELD
                     GLQSMLKLVKGLPEVVAPPDFYEKIAKKVRKRRGRLEVLWMLALPFQVLSVVLILVVA
                     VTYMLLHLDNDPSVKLERDPTVQPHKPAP"
     gene            complement(642..1376)
                     /locus_tag="VGB85_08690"
     CDS             complement(642..1376)
                     /locus_tag="VGB85_08690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0134144.1"
                     /translation="MTDLDPSATPRVVDLAAVVEPDEPEPAPPDDSTLTPAQREEAAK
                     RAEQDRRDRRLVRRLKEGDERAFQELVHTYQNRIFGLMLRMINNRQEAEDLAQEVFII
                     VYRAIGSYRGEGRFYTWLYRIASNTCKNRLKYLKGRNFHRSVEIEDSPQAQLPSAEGG
                     AVVTFQSQVPGPEATVEGTRMEQVIQRELGALDPEHRLLIVLRDIQGLSYQDIIRITG
                     LQEGTLKSRLHRARLALKERLQPFMR"
     gene            complement(1466..2584)
                     /locus_tag="VGB85_08695"
     CDS             complement(1466..2584)
                     /locus_tag="VGB85_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134145.1"
                     /translation="MSVSRRPRACVPRPACFARVLALVAVCLAVVIPSRASANPGLAG
                     TRHLGMGGASRASTRGAGAMLVNPANLGFTRQFEIEPAYQGNIESNTHGAGILVMDSL
                     LNERLALGLGYVATIGAPRVSFATDTGDTQKLKLVHTAHEVSLPIAINVALGWLAFGV
                     RPKFQYSAMRFEDAEGVRRDARAEQTKFGLDLALTFSARQWVNISVIGQNISGQNVPA
                     TTLDLAPYSFDLTTLDRSRVSPVSDYARTLAHGIAVFPTRKPTFSINFDGVYDFTSFR
                     YGFKDEDKYTRKLFAGGVEYSIRDVVPLRIGGFYDSRGRGKTDDRGYIALGVGYFRAA
                     PKGAAGFNLGVGVSRQITGPSPETLIAVSLGILLNPQR"
     gene            complement(2672..>3076)
                     /locus_tag="VGB85_08700"
     CDS             complement(2672..>3076)
                     /locus_tag="VGB85_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134146.1"
                     /translation="ALVIGHGVVLPGLSGAPLIDANLGNALAAPLGLRLAEILVGAGV
                     VLVMTVPRWISSRMATTLALLLTGAALAHRLLLLPHLGRLWARVDIIAGRPVDTLAEA
                     QRWTQHQQWLLLAMGLLLVVLAALATRSPAHE"
BASE COUNT          467 a         1036 c         1117 g          456 t
ORIGIN      
        1 cgcccagctc gactacgacc tcaacgcgct gcccgacgtc ggcggcgcga cgcagcggga
       61 gatccacgcg gtgttcgacc tcggcagcaa cacgctcagc ggcgacatca acggcccggc
      121 cggcacctac acgctgaccg gcacgccggg caccggtaac caggcgcgca tcgtcgtcga
      181 catctcgcag aagtgatgtt cagggcgccg gcttgtgcgg ctgcaccgtc gggtcgcgct
      241 cgagcttgac gctcgggtcg ttgtcgaggt gcagcagcat gtacgtgacc gcgacgacga
      301 ggatcaggac gacgctgagc acctggaacg gcagcgcgag catccacagc acctcgaggc
      361 ggccgcgtcg cttgcggacc ttcttggcga tcttctcata aaagtccggc ggggcgacga
      421 cctcgggcag gcccttgacg agcttgagca tgctctgcag gccgtcgagc tcctcgcgcg
      481 cctcgggcga ctctgcgagc agcttctcga ccctcgcggc ctcgtcgggc gcgagctgct
      541 tgtcgaggta ggcgctcagc agcgcgtcgg catcggtggg gcttggctcg gccatggcgc
      601 tcgctcgagg ataacgtttg aacagtggga tcgcacgcgc gtcagcgcat gaacggctgg
      661 aggcgctcct tgagcgccag gcgggcccgg tgcaggcggg acttgagcgt gccctcctgc
      721 agtccggtga tgcggatgat gtcctgataa ctgaggccct gaatgtcgcg gagcacgatc
      781 agcaggcggt gctcggggtc gagggcgccg agctcgcgct ggatgacctg ctccatgcgc
      841 gtgccctcga ccgtcgcctc cggcccgggg acctggctct ggaaggtgac gaccgcgccg
      901 ccctcggccg acggcagctg cgcctgcggc gagtcttcga tctcgacgct gcggtggaag
      961 ttgcggccct tgaggtactt gagccggttt ttgcaggtgt tgctggcgat gcgatacagc
     1021 caggtataaa accgcccctc gccgcggtag ctgccgatcg cccgatagac gatgatgaac
     1081 acctcctgcg cgaggtcctc ggcctcctgg cggttgttga tcatgcgcaa catcaggccg
     1141 aagatccggt tctggtaggt gtgcaccagc tcctggaagg cccgctcgtc gccttccttc
     1201 aggcgccgga ccaaccgccg gtcacgccgg tcctgctcgg cgcgcttggc cgcctcctcg
     1261 cgctgcgcgg gcgtcagggt cgagtcgtcc ggcggcgccg gctcgggctc gtcgggctcg
     1321 accaccgccg cgaggtcgac gacgcgaggc gtcgcgctgg ggtcgaggtc ggtcacgcga
     1381 ctcactttcg atcagaccac cccgcgcgca caaggttcct gcacctgcaa agagcgtccg
     1441 ggatcgcgcc cgggccggat cggcgtcagc gctgcgggtt cagaagaatc cccaggctga
     1501 ccgcgatcag cgtttccggg ctcggaccgg tgatctggcg cgagacgccg accccgaggt
     1561 tgaagccggc cgcccctttc ggcgccgctc gaaagtaacc cacgccgagc gcgatgtagc
     1621 cgcggtcgtc ggtcttgccc cggccccggc tgtcgtaaaa gccgccgatg cgcagcggca
     1681 cgacgtcccg gatcgagtac tcgacgccgc cggcgaacag cttgcgcgtg tacttgtcct
     1741 cgtccttgaa gccgtaacgg aacgaggtga agtcgtagac gccgtcgaag ttgatcgaga
     1801 aggtcggctt gcgggtcggg aacaccgcga tgccgtgcgc cagcgtccgc gcgtagtccg
     1861 acaccgggct gacgcggctg cggtcgagcg tcgtcaggtc gaagctgtag ggcgcgaggt
     1921 cgagggtcgt cgccggcacg ttctgcccgg agatgttctg gccgatgacc gagatgttga
     1981 cccactggcg cgcgctgaag gtcagcgcga ggtcgaggcc gaacttggtc tgctccgcgc
     2041 gcgcgtcgcg gcgcacgccc tcggcgtcct cgaagcgcat cgcgctgtac tggaacttgg
     2101 gccgcacgcc gaacgcgagc cagccgagcg ccacgttgat ggcgatcggc aggctgacct
     2161 cgtgggcggt gtggaccagc ttgagcttct gggtgtcgcc ggtgtcggtc gcgaagctga
     2221 cccgcggcgc gccgatcgtc gccacgtagc cgagcccgag cgcgagccgc tcgttgagca
     2281 gcgagtccat caccaggatc ccggcgccgt gcgtgttgct ctcgatgttg ccctgatagg
     2341 ccggctcgat ctcgaattgc cgcgtgaagc cgaggttggc cgggttgacc agcatcgccc
     2401 cggccccgcg ggtgctcgcc cgcgaggcgc cgcccatccc gaggtgccgc gtgccagcga
     2461 gtccgggatt ggccgaggcg cgtgacggaa tgacaaccgc gagacagacg gccaccagcg
     2521 ccagcacccg cgcgaagcag gctgggcggg ggacgcaggc gcgtgggcga cgggagacgg
     2581 acatggcggg gcgcgagatg caggagcccg agcaagttcc gggccagatc tcccggggag
     2641 gtaacacggg cccgcggaac ggaccagcgg cttactcgtg ggccgggctg cgcgtcgcca
     2701 gggcggcgag gacgaccagc agcagaccca tggcgagcag cagccactgc tggtgctgcg
     2761 tccagcgctg ggcctcggcg agggtgtcga ccggccggcc cgcgatgatg tcgacgcgcg
     2821 cccacaggcg cccgaggtgc ggcagcagca gcaggcgatg ggcgagggcg gcgccggtga
     2881 gcagcagcgc gagggtggtc gccatccgcg aggagatcca ccgcgggacg gtcatcacca
     2941 gcacgacgcc ggccccgacg aggatctccg cgaggcgcag cccgagcggg gccgcgagcg
     3001 cgttgccgag gttggcgtcg atcagcggcg cgccggacag ccccggcagc accaccccgt
     3061 gaccgatcac cagggc
//
LOCUS       DASWNJ010000514         3301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_150337, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000514 DASWNJ010000000
VERSION     DASWNJ010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150337"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..1319)
                     /locus_tag="VGB85_08705"
     CDS             complement(96..1319)
                     /locus_tag="VGB85_08705"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0134147.1"
                     /translation="MNKSIAGLSLLLISLVVGCAGSGGTPAGPATVAGPTCDAGECNT
                     RGMEAVTLARYADAIVLLDRACALGHAVGCSNLAGLMRGGAGGLGQPSRTVGLYDRAC
                     TLGFAEACAAVGAIYAEGKLVAADPVKALAMYDRACTQKDAQGCFTAGIFHNEGRGGP
                     RSPEAAAQRFDRGCELGHATACFNAGVLLYRERGARPGENERAAGYFARACEGQQPAG
                     CLRLGLATLRGHGTSPDVQVAKDLFDRACAGGEDDGCAAARQLAKGKGRRDLEIALTS
                     SAPTLAIAGLRVHDLVCRMPEVGPMALAEAVEAVAAHKAGLDACAPQGMATAVTWQWK
                     GRRAAQVTARGGGPKVEACVRKAVERARAELTGSCAAVFLIGDPEGARRRLTEQRSAG
                     AVPIKLARAGATPGA"
     gene            1506..1901
                     /locus_tag="VGB85_08710"
     CDS             1506..1901
                     /locus_tag="VGB85_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134148.1"
                     /translation="MLLAACDGGTPEDASTGSDCSTPPASEECIDTLVQQCSALSKAD
                     CAAHADGASCLWAIELTGDAMCIQGAGACVAVQYPGEGGPGPRWFDANDRVYDVQCDA
                     TACKDLVPTVYRMDPCDAVDMSPAACNCT"
     gene            complement(1955..3049)
                     /locus_tag="VGB85_08715"
     CDS             complement(1955..3049)
                     /locus_tag="VGB85_08715"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0134149.1"
                     /translation="MAGVCADGEPSPGEFCFDFHDTPEVIAPILVSGARFATELPPAI
                     AVFSAGNTATLVRWDNDQTEVTPLIPKISPASYVRALPVDVQDSEEPELVISHLWGAS
                     LSPNLAGELQALVPVGFPEEAFGLPGLSVPVDLDGDKKPEYIKGAGKKLELWRKPLDT
                     WVKDPSFGFEVPGCKFLADFALGDFNDDGFADLAYIGAADADGDADCASASAQGIAIL
                     LRTEPGGFVAAPAISTGAHAYRNVKAGDFDADGRDDIASLGSNDELLLFRSTGDGEFA
                     SPLILGGVLSFVVGNLDGDSASECLLNRKGQGVVIIDRIFAATTEMPAPNLLGHPLAA
                     IDLNHDGVDDIAFLWEGLEAPSLGIAISTP"
BASE COUNT          567 a         1194 c         1034 g          506 t
ORIGIN      
        1 agagccccga gtcccgcgat caagccaatg cataagcgaa gcgtcatggc cgagaggcta
       61 cgcagcggcc gcacgcccgt caatgcgccg cgacgtcacg cgcccggcgt cgcgccagcg
      121 cgggccagct tgatcgggac agcgcccgca gaacgttgct ccgtcagcct ccgccgcgct
      181 ccctccgggt caccgatcag gaacaccgcc gcgcagctgc ccgtcagctc cgctcgcgcc
      241 cgctcgaccg ccttgcgcac gcacgcctcg accttcggcc cgccgccacg cgccgtcacc
      301 tgcgccgcgc gccggccctt ccactgccac gtcaccgccg tcgccatgcc ctgcggcgcg
      361 caggcgtcga gcccggcctt gtgcgccgcc acggcctcga ccgcctccgc gagcgccatc
      421 ggcccgacct ccggcatgcg gcacaccagg tcgtgcacgc ggagccccgc gatcgccagc
      481 gtcggcgcgc tcgaggtcag cgcgatctcg aggtcgcgcc gacccttgcc cttcgccagc
      541 tgccgcgccg ccgcgcagcc gtcgtcctcg ccgcccgcac atgcccgatc gaacaggtcc
      601 tttgcgacct gtacatcggg actggtgccg tgcccgcgca gcgtggcgag gccgagccgc
      661 aggcagcccg ccggctgctg gccctcacac gcgcgcgcga agtagccggc cgcgcgctcg
      721 ttctcccccg gccgcgcccc gcgttcgcgg tacagcagga cccccgcgtt gaagcaggcc
      781 gtcgcgtgcc cgagctcgca gccgcggtcg aagcgctgcg ccgccgcctc cgggctgcgc
      841 ggcccgccgc gcccctcgtt atgaaagatg ccggccgtga agcagccctg cgcgtccttt
      901 tgcgtgcacg cgcggtcgta catcgcgagc gccttgaccg ggtcggccgc gacgagcttg
      961 ccctccgcgt agatcgcccc gaccgccgcg cacgcctccg cgaagccgag cgtgcacgcg
     1021 cggtcgtaca gcccgaccgt gcgcgacggc tggccgaggc ccccggcccc gccgcgcatc
     1081 agccccgcga ggttcgagca gccgaccgcg tggcccagcg cgcacgcccg gtcgagcagc
     1141 acgatcgcgt ccgcgtagcg ggccagcgtc accgcctcca tcccgcgggt gttgcactcc
     1201 cccgcgtcac aggtcggccc cgcgaccgtc gccggccccg caggcgtgcc gccagagccg
     1261 gcacacccga ccacgagcga gatcaacagc aacgacagcc cggcgatcga cttgttcacg
     1321 tgcgcgtctc cgcccgtgag gatgctggcc cccaccacgc cggtcaagcg caacgatcgc
     1381 taccaggcgc cgtggcgccg gccaaggaat acacctacgg ttgcatcgca aactaaacag
     1441 cgctcttttt gggctgccct taccggtaca aacacatgaa aaataccagc tatttgcttg
     1501 cgatcgtgct cttggccgcg tgcgacgggg gcacccccga ggacgcatcg accggctcgg
     1561 actgttcgac cccgcccgcg tcggaggaat gcatcgatac gctcgtgcag caatgctccg
     1621 ccctctcgaa ggccgactgc gcggcccatg cggacggcgc gtcgtgcctc tgggcgatcg
     1681 agctgaccgg cgacgccatg tgcatacagg gtgccggggc ctgcgtcgcc gtccagtacc
     1741 ccggcgaggg cggcccgggc ccgcgctggt tcgacgcgaa cgaccgcgta tacgacgtcc
     1801 agtgcgacgc gaccgcctgc aaagacctgg tgccgacggt ctatcgcatg gacccctgcg
     1861 acgcggtcga catgtcgccc gccgcctgca actgcacctg acggatgact ctccgccagg
     1921 caccgcccat gctatcccac gacggcggac ggtgctacgg cgttgagatc gcaatgccca
     1981 gagaaggtgc ttcgaggcct tcccagagga aggcgatgtc gtcaaccccg tcgtgattca
     2041 ggtcgatcgc agccagggga tggcccaaga ggttgggggc cggcatctct gtggtagccg
     2101 caaagattcg atcgataatt acgactccct gccccttcct gttgagcaaa cactctgaag
     2161 ccgagtctcc gtccagattt ccgacgacga aagagagaac cccgcccagg atcagcggag
     2221 aggcgaactc cccgtcccct gtcgagcgaa agagcaataa ttcgtcgttg ctgccgagcg
     2281 aggcgatatc atcgcggcca tcagcgtcga agtcgcctgc tttgacgttg cggtaggcgt
     2341 gcgcccccgt cgagatcgcc ggtgcggcga cgaatccacc cggttcggtt cgcagaagga
     2401 tcgctatacc ttgcgccgat gcagaggcac aatctgcgtc accgtcggca tcggcggcgc
     2461 cgatatacgc tagatcggcg aaaccgtcat cgttgaagtc tccgagggca aaatcagcca
     2521 ggaacttgca gccggggact tcaaagccaa atgatggatc tttcacccat gtatcgagcg
     2581 gcttgcgcca gagctcgagc ttcttgcctg ctcccttgat gtattctggc ttcttgtcgc
     2641 cgtcgagatc gacgggcact gaaagcccgg gtaggccgaa cgcttcctcc gggaatccca
     2701 cagggacgag ggcctgaagt tctccggcca gattcggtga gagcgatgca ccccacaggt
     2761 gcgaaatcac caattccggc tcctcagaat cctgcacgtc gaccgggaga gcccgaacgt
     2821 atgaagcagg cgaaatcttc gggatcagcg gagttacttc ggtttggtca ttgtcccagc
     2881 gcaccagagt agccgtgtta ccggcagaga aaacggctat cgcaggcggg agctcggtgg
     2941 cgaaccgcgc accggatacg aggattggcg cgataacttc tggcgtatca tggaaatcga
     3001 agcaaaattc gccaggactc ggctcgccgt ctgcacagac tcccgccata ggcgcagtat
     3061 cagacgcagt tggcggcgag ttaagtcctg gtgcgcacga tgcgagagcg aagcagattg
     3121 ggacactacc tcgacgtctc tgcatgagcc ttcttagcac gagaggcgga cactgagcgc
     3181 gtcgaaacta gcgcaacgtg cgcctgtcag agcggaccgt tggcctcgca gatctgcttg
     3241 tatcgacggc cgggtggccg cacgcctgga cagcgtgcca gtcggcgcgt cgctacctga
     3301 g
//
LOCUS       DASWNJ010000515         2126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_44589, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000515 DASWNJ010000000
VERSION     DASWNJ010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44589"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VGB85_08720"
     CDS             complement(<1..467)
                     /locus_tag="VGB85_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134150.1"
                     /translation="MKDEAGAPARRSALAGLARVVLAGLVAAALTWQIARGWPAPPVE
                     RATLASVAFIPLWAGLALALLAGGRRSRRGGARRRLFALHRRLGGALVLVAVIVFGSG
                     VGAVLDRSLARWQVEHAPVRDVPALSEQRLDAVLAAVLADHPALTHGELALHPA"
     gene            complement(464..1765)
                     /locus_tag="VGB85_08725"
     CDS             complement(464..1765)
                     /locus_tag="VGB85_08725"
                     /inference="COORDINATES: protein motif:HMM:NF024826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SidA/IucD/PvdA family monooxygenase"
                     /protein_id="HEY0134151.1"
                     /translation="MHDEIDLAGVGVGPFNLSIAALADELPELRCRFFEQRERFAWHP
                     GLMFRGARMQTSFLKDLVTAVSPTNPHSFVNYLVQHGRFHAFLAAESAAIERREFADY
                     LGWVAGRLGSLRFGDGVQAVHLVPGGFELLHTGGRTRARHVVLGTGRVPSVPRCALGR
                     LGPRCFHAIDILSRPPSLTGARVAVIGGGQTGAEVVLELLRGAWGEPAELSWISRRLN
                     FEPLDDSPFTNHLFTPGFVNAFHGLGRARREQLLARHKLAGDGISLATLRALHDRIYE
                     MHALREGPQVELLPSRELVAIDEHGPGFSLQLHNLLDDEREALAVDWVILCTGLRTQL
                     PPCVEPLLPLLQRDDRGDLQVDANFVVGAASGAGRLYIVNGGRTSHGVAESQLSLMAW
                     RSAVILNDVLGRPRFAVEHERELVRWRARPTGAGGYDARSR"
     gene            complement(1758..>2126)
                     /locus_tag="VGB85_08730"
     CDS             complement(1758..>2126)
                     /locus_tag="VGB85_08730"
                     /inference="COORDINATES: protein motif:HMM:NF018031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IucA/IucC family C-terminal-domain containing
                     protein"
                     /protein_id="HEY0134152.1"
                     /translation="LDHEAALRGLRALPGEQIVHDLYTGYFIGVLRFVAPLLTRAFGL
                     PERAFLAALAGALRSYQHAHPALQARFAALDLFRPTMVRICLNRARLQAGHGDGAARP
                     LPELGPPLANPLLAEDNHDA"
BASE COUNT          336 a          756 c          801 g          233 t
ORIGIN      
        1 gccggatgca gcgcgagctc gccgtgcgtc agcgccgggt ggtcggcgag caccgcggcg
       61 agcaccgcgt cgagccgctg ctccgacaac gccgggacgt cgcgcaccgg cgcatgttcg
      121 acctgccagc gggcgagcga gcggtcgagc acggcgccga cgcccgagcc gaacacgatc
      181 acggcgacga gcaccagcgc cccgccgagc cgacggtgca gcgcgaacag ccgccgccgc
      241 gccccacccc gccgcgatcg ccggcccccg gccagcaagg cgagcgccag accggcccac
      301 agcgggatga aggcgacgct cgcgagggtc gcgcgctcga ccggcggcgc gggccagccg
      361 cgcgcgatct gccaggtgag cgcggcggcg acgagccccg cgagcacgac gcgagcgagg
      421 ccggcgagcg cgctccgcct cgcgggagcc cccgcttcat ccttcatcgc gacctcgcgt
      481 cgtatccacc ggcaccggtc ggtcgtgcgc gccagcggac cagctcgcgc tcgtgctcga
      541 cggcgaagcg cgggcggccg agcacgtcgt tgaggatgac ggccgagcgc caggccatca
      601 ggctcagctg cgactcggcg acgccgtggc tggtgcggcc gccgttgacg atgtagagcc
      661 ggccggcccc ggaggcggcg ccgaccacga agttcgcgtc gacctgcaga tcgccgcggt
      721 cgtcgcgctg cagcagcggg agcagtggtt cgacgcacgg aggcaactgc gtgcgcagcc
      781 ccgtgcaaag gatcacccag tcgaccgcga gcgcctcgcg ctcgtcgtcg agcaggttat
      841 gcagctgcaa cgagaagccg ggcccgtgct cgtcgatcgc caccagctcg cgcgacggca
      901 ggagctcaac ctgcggcccc tcgcgcagcg cgtgcatctc ataaatgcga tcatgcaggg
      961 cgcgcagggt cgccagcgag atgccgtcgc cggcgagctt gtgccgcgcg agcaattgct
     1021 cgcggcgggc ccgcccgagg ccgtggaagg cgttgacgaa gccgggtgtg aacaaatggt
     1081 tggtgaacgg gctgtcgtcg agcggctcga agttgaggcg ccgggagatc cagctgagct
     1141 cggcaggctc gccccacgcg ccgcgcagca gctcgagcac gacctcggcg ccggtctgtc
     1201 cgccgccgat cacggccacg cgcgcgccgg tcaggctcgg cgggcgcgag aggatgtcga
     1261 tcgcgtggaa gcagcgcggg ccgagccggc cgagcgcgca gcgtggcacc gacgggaccc
     1321 ggcccgtgcc gagcacgacg tgccgcgccc gcgtgcgccc gccggtgtgc agcagctcga
     1381 agccacccgg caccaggtgc accgcctgca cgccgtcgcc aaagcgcagc gagccgagcc
     1441 gcccggcgac ccagccgaga taatcggcga actcccgccg ctcgatcgcc gcgctctcgg
     1501 ccgcgaggaa cgcgtgaaag cggccgtgct ggaccaggta gttgacgaag gagtgcgggt
     1561 tggtcgggct gaccgcggtg accaggtcct tgaggaacga ggtctgcatg cgagcgccgc
     1621 ggaacatcag gcccggatgc cacgcgaagc gctcgcgctg ctcgaagaag cggcagcgca
     1681 gctcgggcag ctcgtcggcg agcgcggcga tgctgagatt gaacgggccc acgcccacac
     1741 cagcgagatc gatctcatca tgcatcgtgg ttatcctcgg cgagcagggg gttcgcgagc
     1801 ggcggtccga gctcgggcag cggacgcgca gcgccgtcgc cgtggccggc ctgcaagcgc
     1861 gcgcggttga ggcagatccg gaccatcgtc gggcgaaaca ggtcgagcgc ggcgaagcgg
     1921 gcctgtagcg cggggtgagc gtgttgatag ctgcgcaacg cgccggcgag cgccgccagg
     1981 aaggcccgct cgggcaggcc gaaggctcgt gtcagcagcg gcgcgacgaa gcgcagcacg
     2041 ccgatgaagt agccggtata gaggtcgtgc acgatctgct cgccgggcag cgcccgcagg
     2101 ccgcgcagcg ccgcctcgtg atcgag
//
LOCUS       DASWNJ010000516         1768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_679315, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000516 DASWNJ010000000
VERSION     DASWNJ010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679315"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="VGB85_08735"
     CDS             <1..719
                     /locus_tag="VGB85_08735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY0134153.1"
                     /translation="IHGIVQPIVVTPHDDPPGSFMIVAGERRWRAAQLAGHHDVPVVV
                     RDTPESDRLELAVLENLQRLDLSPIEEAAAYRQLMDVREYTQEQLAERLGKDRSTISN
                     ALRLLKLPPKVQGYVQEGRLSMGHARALLALESPADMIELAMEAMQRGYTVRAMERAV
                     KARLHPPPVPAEPSEAERAREVIVRDLESRLQRGLGVKVAVKTNAQRQGSGTIELPYG
                     SLDELDRLLAMLLPPDTREL"
     gene            complement(723..1577)
                     /locus_tag="VGB85_08740"
     CDS             complement(723..1577)
                     /locus_tag="VGB85_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134154.1"
                     /translation="MTVALVTAGAARSLDTDLPLLQAALTRLGIDSEVVSWDDATLVW
                     GRFATIVVRSTWDYTGRLDTFLEWAASAAEAAPLFNPRPLLQWSTDKRYLADLAAAGV
                     AIVPTRFCARGSAAELPDRPFVVKPSVGAGSVGVARFTPDEREAALEHVARLHDAGHV
                     ALIQPYLDAVDRRGETALVYFAGSFSHAIRKSAILVEGLQMVDGLYAAEKIARAQPTT
                     AELALAEAALAAVPGRDRPLYARVDVVPAEDGTPRLLELELCEPSLFLEYDEGAADRL
                     AAAIAALT"
BASE COUNT          250 a          655 c          655 g          208 t
ORIGIN      
        1 agatccacgg gatcgtccag ccgatcgtcg tcaccccgca cgacgacccg ccgggcagct
       61 tcatgatcgt cgccggcgag cggcgctggc gggccgccca gctcgccggt caccacgacg
      121 tcccggtcgt cgtccgcgac acgcccgaga gcgaccgcct cgagctcgcc gtgctcgaga
      181 acctccagcg cctcgacctc agcccgatcg aggaggccgc cgcgtaccgc cagctgatgg
      241 acgtccgcga gtacacgcag gagcagctcg ccgagcgcct cggcaaggac cgcagcacga
      301 tctccaacgc gctgcgcctg ctcaagctgc cgcccaaggt ccagggctac gtgcaggagg
      361 gccgcctgag catgggccac gcacgggccc tgctcgcgct ggagagcccg gcggacatga
      421 tcgagctggc gatggaggcg atgcagcgcg gctacaccgt ccgcgcgatg gagcgcgccg
      481 tgaaggcccg cctgcacccg ccgcccgtgc ccgccgagcc gagcgaggcc gagcgcgcgc
      541 gcgaggtgat cgtccgcgac ctcgagtcac gcctgcagcg cggcctcggg gtcaaggtcg
      601 ccgtcaagac gaacgcgcaa cggcaaggca gcggcacgat cgagctgcct tacgggagcc
      661 tcgacgagct cgaccgcctg ctggcgatgc tgctgccgcc cgacacccgc gagctgtgag
      721 gctcaggtca gcgccgcgat cgcggccgcg agccggtccg ccgcgccctc gtcgtattcg
      781 aggaacagcg acggctcgca cagctcgagc tcgagcaggc gcggggtccc gtcctcggcg
      841 ggcacgacgt cgacgcgggc gtacagcggc cggtcgcggc cgggcaccgc ggcgagggcc
      901 gcctcggcga gcgccagctc ggccgtggtc ggctgcgcgc gcgcgatctt ctcggcggcg
      961 tacaggccgt cgaccatctg caggccctcg acgaggatcg ccgacttccg gatcgcgtgg
     1021 ctgaagctgc ccgcgaagta gaccagcgcc gtctcgccgc ggcggtcgac ggcgtcgagg
     1081 tagggctgga tcagcgcgac gtggccggcg tcgtgcaggc gcgcgacgtg ctcgagcgcc
     1141 gcctcacgct cgtcgggggt gaagcgcgcg acgccgaccg agcccgcgcc gaccgagggc
     1201 ttgaccacga acggccggtc cggcagctcc gcggcgctgc cccgggcgca aaagcgggtc
     1261 ggcacgatcg ccaccccggc ggccgcgagg tcggcgaggt agcgcttgtc ggtcgaccac
     1321 tgcaacagcg ggcgcgggtt gaacagcggc gccgcctcgg ccgcggacgc ggcccactcg
     1381 aggaaggtgt cgaggcgccc ggtgtagtcc caggtcgagc gcacgacgat cgtggcgaag
     1441 cgcccccaca cgagcgtggc gtcgtcccag ctgacgacct cgctgtcgat gcccaggcgc
     1501 gtcagcgcgg cctgcaggag cggaaggtcg gtgtcgagcg agcgcgccgc ccctgcggtg
     1561 acgagagcca cggtcatacc aggtcagcaa acccccggag cgcccggtcg tcaacttcgc
     1621 ggcgcaaccc ctgtcccccg ggccaggtgg gccggcgccg tggcgctgct atgctgggcc
     1681 ggtgcgacgg cgcgggcgct ggcttgtacg cgggtcgctc gcggtcctgg cgtggggcgc
     1741 cgtgcacctc gcggcgatct gcgtcgac
//
LOCUS       DASWNJ010000517         4409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_115194, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000517 DASWNJ010000000
VERSION     DASWNJ010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115194"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VGB85_08745"
     CDS             complement(<1..233)
                     /locus_tag="VGB85_08745"
                     /inference="COORDINATES: protein motif:HMM:NF015678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEY0134155.1"
                     /translation="MRLLGNLLWFVLAGLWLFLAYVGAGVVQCLTIIGIPFGIQSFKL
                     AIFAAWPFGRAVVSLPGDSGGLSCLGNIVWLVLG"
     gene            complement(298..1359)
                     /locus_tag="VGB85_08750"
     CDS             complement(298..1359)
                     /locus_tag="VGB85_08750"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0134156.1"
                     /translation="MTDLTSAIPQGKTLVTGAAGHVGANLVRRLLADGVDVRVLVHPK
                     HNNRGVEALPVERIEGDVRDLDTVRRAVTGCVRVYHTAAKVSVVSPTPTQTRELWEIN
                     VMGTQNVMQSALRAGVARVCLTSSFSTVGFHADDPSRPCAEDLPFYPFIEWMPYSRTK
                     VLAEVETFKAIADGLDAVIAISTGVCGPHDYLPSRTGNVLLNYARGKFRAYIPGGYEA
                     VSVDDLARGHLLAMERGRTGQRYTISSRYVTMDEMLDLFAEVTGKPRNQRKLPPKLMA
                     SIARVVSGTMTALFPDAPQHLTPGAVLVLSMQRRADISKARKELGYEPTPLEPAVRAW
                     HDFFASEGMIAGNVNRIGA"
     gene            1429..2721
                     /locus_tag="VGB85_08755"
     CDS             1429..2721
                     /locus_tag="VGB85_08755"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEY0134157.1"
                     /translation="MRSVTVGILGLGNVGAGTVRLLQERRFAIEARLGAVLEVRRVCV
                     RDMARRRAVEVAPELITTNPQDVIDDPLIDIIVELVGGVEPACTYILAALRAGKHVVT
                     ANKAVLAERGAEIFAAAAAHGRALYFEGSVAGGIPILRSLREGLASDRIDAVAGIVNG
                     TSNFILDAMTRSGADFNEALRGAQAAGYAEADPSLDVGGGDAAHKLALLTLVAFGVRI
                     DPRMIVTEGITGISAFDIRTASELGYVVKSLAIAHETQLGPCLRVHPTLVPRSHVLAG
                     VTGAYNAVLVESRALGRSLYYGRGAGMMPTAVAVVSDLIELARDLVAGVAPPISPSVR
                     ELSPAPMDPLEHENYLCVHVPNIPGVLGRVATCLGRHGVSISRMIQDRPGRGPVPMVI
                     LTEAVSERAMQAALTDLAASPDVATPPTRIRMLPASLV"
     gene            2781..>4409
                     /locus_tag="VGB85_08760"
     CDS             2781..>4409
                     /locus_tag="VGB85_08760"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEY0134158.1"
                     /translation="MVARTMPGTVAGSSPPIQERTTSAIGVEIPGVGLGPAMSIGPAP
                     DWVDKVPAGLPCKGATRVVLLDEQRSWLGPRLQHFTRIIYRVRDAEDARDFKLLRIPL
                     GHDRAEVTVHALSVYRGTEERRHLDPWFTTTTPWQPISKEAEAGPPHVVKDRPATLLH
                     RLIDVEVGDYVEIAHTVVYPVEPELAGVICGSLKLTREVPIQRQHHRILFPNRQVAIR
                     AVAIEGQPIQRRHEDFTELVWYSQNVPALPETLLHERRRASTPHIQYSEIGSWDALVR
                     LVRPFFECDAPPPEIAEIAHRLARDLPSKRAQVRAVTRYVQDELAFEAYDTREWLVRP
                     KPLARVLAEKATDCKGKTFLMLSLLRALGIAAEVVMVNHHGMDLRAALPSLIDLDHMI
                     VMVTVDGHEYFIDPTLRCQRGDLDALTPLPFLYGVPLVLGRGPVEIPHMCARPAPQSE
                     ISARYTVGAPGKPTRLSYQRIDHGADADTARTIQRGDDRVDLGRQLHAGFKRIYPELR
                     AVDRVVTHDDETANSYRLTAELQFGSMWRRDPAGE"
BASE COUNT          698 a         1540 c         1505 g          666 t
ORIGIN      
        1 ccgagcacca gccacacgat gttgccgagg cacgacaggc cgccgctgtc gccgggcagc
       61 gagacgaccg cgcggccgaa cggccacgcg gcgaagatcg ccagcttgaa cgactggatc
      121 ccgaacggga tgccgatgat cgtcaggcac tggaccacgc cggcgccgac gtacgcgagg
      181 aacagccaca ggcccgccag cacgaaccac aggagatttc cgagcagacg catgcgccat
      241 gcatgcgcca tctcgaaccc ccgcgccgct cactcgatcg tgagtcgctg cacgcgctca
      301 ggcgccgatg cggttgacgt tgcccgcgat catgccctcg ctggcgaaga agtcgtgcca
      361 cgcgcggacc gccggctcga gcggggtcgg ctcgtagccg agctccttgc gcgccttgct
      421 gatgtcggcg cggcgctgca tcgacagcac gagcacggcg ccgggcgtca ggtgctgcgg
      481 ggcgtcgggg aacagcgcgg tcatcgtgcc ggagaccacg cgggcgatcg acgccatcag
      541 cttcggcggc agcttgcgct ggttcctcgg cttgccggtc acctcggcga acaggtcgag
      601 catctcgtcc atcgtcacgt agcgcgagga gatcgtgtag cgctggccgg tgcggccgcg
      661 ctccatcgcc agcaggtgac cgcgggcgag gtcgtcgacg ctgaccgcct cgtagccgcc
      721 ggggatgtac gcccggaact tgccgcgcgc gtagttcagc agcacgttgc cggtgcgcga
      781 cggcaggtag tcgtgcggtc cgcacacgcc ggtcgagatc gcgatgaccg cgtcgaggcc
      841 gtcggcgatc gccttgaagg tctcgacctc ggcgagcacc ttggtgcgcg agtacggcat
      901 ccactcgatg aagggataaa acggcaggtc ctcggcgcag gggcgcgagg ggtcgtcggc
      961 gtggaagccg accgtcgaga acgagctcgt caggcacacg cgggcgaccc cggcccgcag
     1021 cgcgctctgc atcacgttct gcgtgcccat gacgttgatc tcccacagct cgcgggtctg
     1081 ggtcggggtc gggctgacga cggagacctt ggcggcggtg tggtacacgc gcacgcagcc
     1141 ggtgaccgcg cgacggacgg tgtcgaggtc tcgcacgtcg ccctcgatgc gctcgacggg
     1201 caacgcctcg accccgcggt tgttgtgctt cgggtggacc agcacgcgca cgtcgacgcc
     1261 gtcggccagc aggcggcgca ccaggttggc gccgacgtgg ccggcggcgc cggtgacgag
     1321 ggtcttgcct tgggggatcg cagaagtaag gtcggtcatg ggcggcgcac tctaaactgc
     1381 cgcggcgcag ttgaccgcgc gaccgacgga ccgctaccgt cccccggcat gcgcagcgtc
     1441 acggtcggaa tcctcgggct cggcaacgtc ggcgctggca ccgtgcgcct gctgcaggag
     1501 cggcgcttcg cgatcgaagc ccggctcggc gcggtgctcg aggtgcggcg ggtctgcgtt
     1561 cgcgacatgg cgcgtcggcg cgcggtcgag gtcgcccccg agctgatcac gaccaacccg
     1621 caggacgtga tcgacgaccc gctgatcgac atcatcgtcg agctggtcgg tggggtcgag
     1681 ccggcgtgca cgtacatcct cgcggcgctg cgggccggca agcacgtggt gaccgccaac
     1741 aaggcggtgc tcgccgagcg cggcgcggag atcttcgcgg ccgcggcggc gcacgggcgg
     1801 gcgttgtact tcgagggctc ggtcgccggc gggatcccga tccttcgcag cctgcgcgag
     1861 ggcctcgcca gcgaccgcat cgacgcggtc gcggggatcg tcaacggcac ctccaacttc
     1921 atcctcgacg cgatgacccg cagcggcgcc gacttcaacg aggcgctgcg cggcgcgcag
     1981 gcggccggct acgcggaggc cgaccccagc ctcgacgtcg ggggcggcga cgcggcccac
     2041 aagctggccc tgctgacgct cgtcgccttc ggcgtgcgca tcgacccgcg gatgatcgtc
     2101 accgagggca tcaccggcat ctcggcgttc gacatccgca ccgcgagcga gctcggctac
     2161 gtggtcaaga gcctggcgat cgcccacgag actcagctag gcccgtgcct gcgcgtgcac
     2221 ccgacgctgg tgccgcgcag ccacgtgctc gccggcgtca ccggggcgta caacgcggtg
     2281 ctcgtcgagt cgcgcgcgct cgggcgcagc ctctactatg gtcgcggcgc cggcatgatg
     2341 ccgaccgcgg tcgccgtcgt ctccgacctg atcgagctcg cgcgcgacct cgtcgccggc
     2401 gtcgccccgc cgatctcacc ctcggtccgc gagctctcgc cggcgccgat ggacccgctc
     2461 gagcacgaga actatctctg cgtgcacgtg cccaacatcc ccggcgtgct cggccgcgtc
     2521 gccacctgcc tcggccgcca cggcgtcagc atctcgagga tgatccagga ccgacccgga
     2581 cgcggacccg tgccgatggt gatcctcacc gaggcggtca gcgagcgcgc catgcaggcc
     2641 gcgctgaccg acctcgccgc ctcgccggat gtcgccacac cgccgacccg cattcgtatg
     2701 ttgcctgcgt cgctggtctg acaaacggcc agctcgctct ttggcgtaac gaccgccgct
     2761 gcgggtgcgc gaattcgtgt atggtggccc gcaccatgcc aggcacagtc gcgggttcat
     2821 cgccacccat ccaggaacgg acaacgagcg ctatcggggt agagataccg ggcgtcggct
     2881 tgggccccgc catgagcatc ggacctgcgc cggactgggt cgacaaggtc ccggcagggc
     2941 tcccctgtaa gggtgccacg agggtcgtgc tcctggacga acagaggagc tggctcggac
     3001 cccgccttca acatttcacg cggatcatct accgggttcg tgacgccgag gacgcgagag
     3061 acttcaagct gctgcgcatc ccgctcggtc acgaccgggc cgaggtcacg gtccatgcgc
     3121 tcagcgtata ccgcggtacg gaggagcgga ggcacctcga tccgtggttc acgacgacta
     3181 ctccctggca accgatcagc aaggaggccg aggctggtcc gccgcacgtc gtcaaggaca
     3241 ggccagcgac cctcctgcac cgcctgatcg atgtggaggt cggcgactac gtggagatcg
     3301 cgcacacggt ggtctatccc gtcgagccgg agctcgcggg ggtgatctgc gggagtctga
     3361 agctcacccg cgaggtgccg atacagcgac aacaccaccg gatcctgttt ccgaaccggc
     3421 aagtcgcgat acgcgccgtc gcgatcgagg gccaaccaat ccagcgccgt cacgaggact
     3481 tcacggagct cgtgtggtac agccaaaacg tcccggcctt gccggagacc ctgcttcacg
     3541 agcgacgccg ggccagtaca ccgcatatcc aatattcgga gatcggcagc tgggacgcgc
     3601 tggtgcggct ggtccggccg ttcttcgagt gtgacgcgcc cccacccgag atcgccgaga
     3661 tcgcacaccg tctggcgcgt gatctcccga gcaagcgcgc ccaggtgcgc gccgtgacgc
     3721 gctacgtgca agacgagctc gccttcgagg catatgacac acgtgagtgg ctcgtccggc
     3781 ccaagccgct ggctcgtgtg ctcgccgaaa aggccaccga ttgtaagggt aagactttcc
     3841 tcatgctatc gctgctgcgc gcgctcggta tcgcggccga ggtagtgatg gtcaatcacc
     3901 atggcatgga cctgcgagcg gcgctcccct cgctcatcga cttggaccat atgatcgtga
     3961 tggtcacggt cgacggacat gagtacttca tagacccgac gctgcgatgc cagcgcggcg
     4021 acctcgacgc cctcaccccg ctcccgtttc tttatggcgt ccctttggtc ctcggccggg
     4081 ggccggtcga gatcccgcat atgtgcgctc gcccggcgcc ccagtcggag atctcggccc
     4141 gctacacggt cggcgcgccc ggcaaaccga cacgattgag ctaccagcgg atcgatcacg
     4201 gcgcagacgc cgatacggcc cgcacgatcc agcgcggcga cgatcgagtg gatctcgggc
     4261 ggcaattgca cgctggattc aaacggatct atcccgagct ccgcgccgtc gatcgcgtcg
     4321 tgacgcacga cgacgagacc gcgaactcgt atcgtctcac cgcggagctc cagttcggga
     4381 gtatgtggcg tcgcgaccct gccggcgag
//
LOCUS       DASWNJ010000518         3483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_679343, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000518 DASWNJ010000000
VERSION     DASWNJ010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679343"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="VGB85_08765"
     CDS             complement(<1..1233)
                     /locus_tag="VGB85_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134159.1"
                     /translation="MPLLEMLQVLSRTPVTEPQLRAVVAVTCARWCVARGEDGEAQRL
                     LTHALELVPDLRPAMRLLYRIFLRRGDIRNSVRYLDQEIRATRHPREAAALYRERGQL
                     VEAHFGDRSAALQCYQAALKATPRDLAVLRSVEAVALTQGNIFALIANLEAQLEVLHD
                     ARAVAAVARDLALLEARHSGDLNLACELLLAAAEAQPANLSLLGDLFRLAEASGNPDL
                     MLRAIELEADARPPDQRAMALTRASLTLRENRERGAAVSLLRAAANAQPGNISLWRNL
                     EELSMATSRYDVAVEACAGQLKVIGNEDTALQAELFYRLGKLAMTRLDRVSEGLAAMR
                     RALKLFPGHIPALEDAGRYLIANDAWAQLLELLKLQVATADDAGLSPEEKAQAQLRAG
                     QVLEENLGELEGARQIYEE"
     gene            complement(1294..2028)
                     /locus_tag="VGB85_08770"
     CDS             complement(1294..2028)
                     /locus_tag="VGB85_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134160.1"
                     /translation="MASPPPPTDEARAKAVTRLVSDLEQNFRDAVQRSLHFALDGSVT
                     SLAIVDHYLEQARDEQRAPILALLAAGAGAYFGELVRRELGGLWIGDGSDPRHLRLLL
                     TSEFAYFSPVDVAFAAIVGEDPDPDDPRTPPGVPLDISIHLRDTDQPPPNPRRVDDPE
                     DPPADPPETVTELPPLPYDDGSDDASWISARLAELAPVPADQFYSLTTRYETLELILQ
                     LLASRRALGGYEPYTYTVQDYLHAFS"
     gene            2077..>3483
                     /locus_tag="VGB85_08775"
     CDS             2077..>3483
                     /locus_tag="VGB85_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134161.1"
                     /translation="METGVDEWSWSAEVPYACACGAYSPVTRWRWVDADAQPELAKRL
                     RDSGPLAGNCRACQRPAEGGGAWLELSPATEQATLVLAAHQRGELLDALQSHLELLRQ
                     RPAQVRPWLLQPRLAFGTARPASTGIDERGAGVPRPVGQRGWASGPALSAEIERALVC
                     DLTLEAGAVTAVAVLDEPTRRMWGSAALQARPVLLRGLGYPLLGVRLVASYLGEVAVI
                     DAVVDIGEPRSSEVFMRLAQSFRLQLSIRSDTGPATVREIEAESLRRNAAMCLESAQA
                     QLGAGEFPPDAYRTARDSLARMSAKQRLEPARNTLVEGSYRHLVSPAEALAALERLDQ
                     ASKKDNLARLLEVDGLPVGEYEQMRKTVLAAAVEFGLPAPARFWRRTLGADLVRDPAD
                     WITRLVSNRGAWIARGDDLTPEQAEQTWRAILDLCQHHDVSPPPELTQALGGRRDAPK
                     ADGREAARPGVNVASARVA"
BASE COUNT          471 a         1172 c         1354 g          486 t
ORIGIN      
        1 ctcctcgtag atctggcgcg cgccctcgag ctcgccgagg ttctcctcga gcacctgacc
       61 ggcgcgcagc tgggcctggg ccttctcctc ggggcttagg ccggcgtcgt cggcggtcgc
      121 gacctgcagc ttgagcagct cgagcagctg ggcccacgcg tcgttggcga tcaggtagcg
      181 gccggcgtcc tcgagcgcgg ggatgtgccc ggggaacagc ttgagcgcgc gccgcatcgc
      241 cgcgaggccc tcgctgacgc ggtcgaggcg ggtcatcgcg agcttgccga ggcggtagaa
      301 cagctcggct tgcagcgcgg tgtcctcgtt gccgatcacc ttgagctggc cggcgcaggc
      361 ctcgacggcg acgtcgtagc gcgacgtcgc catgctcagc tcctcgaggt tgcgccacag
      421 gctgatgttc cccggctgag cattggcggc ggcgcgcagc aggctgaccg cggcgccgcg
      481 ctcccggttc tcgcgcagcg tcaggctcgc gcgtgtcagg gccatcgcgc gctggtccgg
      541 cggtcgggcg tcggcctcga gctcgatcgc gcgcagcatc aggtccgggt tgccgctggc
      601 ctcggcgagg cggaacaggt cgccgagcag gctgaggttg gcgggctggg cctcggcggc
      661 ggcgagcagc agctcgcacg cgaggttgag gtcgccggag tgcctggcct cgagcagcgc
      721 gaggtcgcgg gcgacggcgg cgacggctct ggcgtcgtgc aggacctcga gctgggcctc
      781 gaggttggcg atcaaggcga agatgttgcc ctgcgtcagc gcgacggcct cgacggagcg
      841 cagcacggcg aggtcgcgcg gggtcgcctt gagggcggcc tggtagcact gcaacgcggc
      901 cgagcgatcg ccgaagtgag cctcgacgag ctgcccgcgc tcgcggtaga gggccgcggc
      961 ctcgcgaggg tgacgcgtcg cacggatctc ctggtcgagg tagcggaccg agttgcgaat
     1021 gtcgccgcga cgcaaaaaga tccgatagag cagccgcatc gccggccgca ggtccggcac
     1081 gagctcgagc gcgtgcgtca gcaggcgctg ggcctcgccg tcctcgcccc gcgcgacgca
     1141 ccagcgagca caggtgacgg cgaccaccgc acgcagctgc ggctcggtca cgggggtacg
     1201 cgacagcacc tgcagcatct ccagcagggg catttcttca gccgcggaca tccgtgctgc
     1261 gcagtctaca ctcccccggg ggggctcgaa actctaggaa aacgcgtgta aatagtcctg
     1321 gaccgtgtag gtatagggct catatccgcc cagggcccgg cgactggcga ggagctggag
     1381 gatcagctcc agggtctcgt agcgggtcgt gaggctgtaa aactggtcgg cgggcacggg
     1441 ggccagctcg gcgaggcggg cggagatcca gctggcgtcg tcgctgccgt cgtcatacgg
     1501 gagcgggggg agctcggtga ccgtctcggg gggatcagcg ggaggatcct ccgggtcgtc
     1561 gacgcggcgc gggttcggcg gcggctggtc ggtgtcgcgg aggtggatgc tgatgtcgag
     1621 cgggacaccc ggcggcgtgc gcgggtcgtc ggggtccggg tcctcgccga cgatcgccgc
     1681 gaaggcgacg tcgaccggcg agaagtaggc gaactcggag gtcaacagca gacgcaggtg
     1741 gcgtgggtcg ctgccgtcgc cgatccacag accaccgagc tcgcggcgca cgagctcgcc
     1801 gaagtaggcg cccgcgcccg cggcgagcag cgcgaggatc ggggcccgct gctcgtcgcg
     1861 cgcctgctcg agatagtgat cgacgatcgc cagcgaggtg accgaaccgt cgagcgcgaa
     1921 gtgcagcgag cgctgcacgg cgtcgcggaa attctgctcg agatcgctca ccagtcgggt
     1981 gacggctttg gcgcgcgcct cgtcggtcgg cggtgggggg cttgccatgg ggccggagct
     2041 ttacactaca aaagtcgagg cgctatggtc cccggcgtgg aaaccggcgt cgacgagtgg
     2101 tcctggagcg ccgaggtgcc ctacgcctgc gcttgcggcg cgtacagccc ggtgacccgc
     2161 tggcgctggg tcgacgccga tgcccagccc gagctcgcca aacgcctgcg ggacagcggt
     2221 ccgctcgcgg gcaactgccg ggcctgccag cgtccggccg agggcggcgg ggcgtggctc
     2281 gagctgtcgc cggcgaccga gcaggcgacg ctggtgctgg cggcgcacca gcgcggcgag
     2341 ctgctcgacg cgctgcaatc tcacctcgag ctgctgcgcc agcgcccggc ccaggtccgt
     2401 ccctggctgc tgcaacctcg cctggccttc gggacagccc ggccggcgtc gaccgggatc
     2461 gacgagcgcg gggccggggt gccccgtccg gtcgggcagc gcggctgggc ctcgggtccg
     2521 gcgctcagcg cggagatcga gcgggcgctg gtctgtgacc tgaccctcga ggcaggcgcg
     2581 gtgacggcgg tcgcggtgct cgacgagccg acccggcgca tgtggggctc ggcggcgctg
     2641 caggcccgcc cggtgctgct gcgtgggctc ggctatccgc tgttgggtgt gcggctggtc
     2701 gcgtcgtacc tcggcgaggt cgcggtgatc gacgcggtcg tcgacatcgg cgagccgcgc
     2761 tcgagcgaag tcttcatgcg cctggcgcag tcgttccgcc tgcagctgtc gatccgcagt
     2821 gacacgggcc cggcgacggt gcgcgagatc gaggccgaga gcctgcgccg caacgccgcg
     2881 atgtgcctcg agtcggccca ggcccagctc ggcgccggcg agtttccgcc cgatgcctat
     2941 cgcaccgccc gcgactcgct ggcccggatg agcgcgaagc agcgactcga gccggcccgc
     3001 aacacgctgg tcgaggggtc gtaccgccac ctcgtcagcc cggcggaggc cctcgcggcg
     3061 ctcgagcggc tcgatcaggc gtcgaagaag gacaacctgg cgcggctgct cgaggtcgac
     3121 ggcttgccgg tcggcgagta cgagcagatg cgcaagacgg tgctcgcggc ggcggtcgag
     3181 ttcggcctgc cggcgccggc ccgattttgg cggcgaacgc tcggcgcaga cctggtgcga
     3241 gatcccgcgg actggatcac acgcctggtg agcaaccgcg gcgcctggat cgcccgcggg
     3301 gacgacctga cgcccgaaca ggcggaacag acctggcgcg cgatcctcga tctgtgccag
     3361 catcacgacg tcagcccacc gcctgagctg acgcaggcgc tcggcgggcg ccgcgacgcg
     3421 ccgaaggcgg acgggcgcga ggcggcccgc cccggcgtga atgtggcgtc ggcccgggtc
     3481 gca
//
LOCUS       DASWNJ010000519         3239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_324285, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000519 DASWNJ010000000
VERSION     DASWNJ010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324285"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..606
                     /locus_tag="VGB85_08780"
     CDS             37..606
                     /locus_tag="VGB85_08780"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY0134162.1"
                     /translation="MFWQALFDPFSILVVVAVGVMLGLSLVWLRGAARQRDLLIGGLM
                     VLRRIGAQLADNHPIRRRLESAPVVDLSFEEIARLMSGQKVEPAARTLIRLGERLAWI
                     ERFAQLAVHLGILGTVFALVSSDPTDLEGFRARLPMALGTTFWGLIGAIALSIVAGAC
                     ESLLERAGQHVREALLEGLEQPPAAEARP"
     gene            603..1307
                     /locus_tag="VGB85_08785"
     CDS             603..1307
                     /locus_tag="VGB85_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134163.1"
                     /translation="MKRRRRHGLDLLPLLDVFMVVLFVFATIQEQQLDTSQQAQAILR
                     SELASAQAQAQARPEPPDPAVEQALRSELAQARDEAVELRDALARLRRAAESAAATGT
                     TGGAEVLRRQEVLNKLVDHFSVFEIEIDGRLVDGVIHNRCCYRTEPMSEQWLPCGEAP
                     AATTELRDWLAEGGGGLVDALRRTKGGNAMTIVRQNELATYRIGRKLEEQLRERFPEH
                     KIYDEGVATHSLACCD"
     gene            complement(1320..2045)
                     /locus_tag="VGB85_08790"
     CDS             complement(1320..2045)
                     /locus_tag="VGB85_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134164.1"
                     /translation="MATGHTSSEGNHTRVRKRSGRSYIVRRAKDDHDGPDADRSVSIR
                     TEKLWRLGTLEEGTIFHASHKHRKRKWVWGYATSGPGGTPHLSYAAHSRCGWVLRANL
                     EPFRRGHEPPYKKPPISYKRWNDRVYRSIQFLWVNYGRFRPTEEQRREHKPGTRRKNS
                     PVPTRRAADVITAYKNSGGRGESHPVLGPVGVRYLNTSGYYMAWARGVRWCFIKASDL
                     HPPVLSEARAKRYTSAQARRRGG"
     gene            complement(2048..2614)
                     /locus_tag="VGB85_08795"
     CDS             complement(2048..2614)
                     /locus_tag="VGB85_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134165.1"
                     /translation="MRIKVEFPDGAPPPRLARIALLDNRVHRVVNPRRQHVATFHDLP
                     AGVTHATITVAGQSVGRQRVVIPEKGEVEVTLRARGGADDDGFAYHGGFRVLDEHSGE
                     PLRGRKYRITSASGINVTGETDENGDTRRIGTHEVEELHLELIDDDDDGLNLLHPDDE
                     DDDDDDDDDDDTEVDDDFGPDADEREED"
     gene            complement(2813..>3239)
                     /locus_tag="VGB85_08800"
     CDS             complement(2813..>3239)
                     /locus_tag="VGB85_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134166.1"
                     /translation="DFNLACDPLSPGGDKCPYDPKAMCEPQSPECAETCGTPNGCDCF
                     GCCTIVIGGVSHDIFIGDQTCSADKIEDCEVCTKNDDCDDECDPMGCEVCFGGELPPG
                     CDDPTCEGGIACTFDQQGNSDCAEGTYCQTGCCVPIVPG"
BASE COUNT          440 a         1053 c         1212 g          534 t
ORIGIN      
        1 gtcaaggtca cggtgctcgc ggactagggg gcggccgtgt tctggcaggc cctgttcgac
       61 ccgttcagta tcctcgtggt ggtggccgtc ggcgtgatgc tggggctgtc gctggtgtgg
      121 ttgcgcggcg cggcgcgcca gcgtgacctg ctgatcggcg ggctgatggt actgcgccgg
      181 atcggcgcgc agctggcgga taaccatccc attcgcaggc gcctcgagtc ggcgccggtg
      241 gtcgaccttt cgttcgagga gatcgcgcgg ctgatgagcg gacaaaaggt cgagcccgcc
      301 gcccgcacgc tgatccgcct cggcgagcgc ctcgcctgga tcgagcgctt cgcccagctc
      361 gccgtgcacc tcgggatcct cggcaccgtg ttcgcgctcg tgagctccga tccgaccgac
      421 ctcgagggct ttcgcgcccg cctgccgatg gcgctcggga cgaccttctg gggcctgatc
      481 ggcgcgatcg cgctgtcgat cgtcgcgggg gcctgcgaga gcctgctcga gcgcgcgggc
      541 cagcacgtcc gcgaggccct gctggagggg ctcgagcagc cgccggccgc ggaggcgagg
      601 ccgtgaagcg gcgccgccgg cacgggctcg acctgctgcc gctgctcgac gtgttcatgg
      661 tcgtgctgtt cgtgttcgcg acgatccagg agcagcagct cgatacttcg cagcaggcgc
      721 aggcgatcct gcgcagcgag ctggcgagcg cgcaggcgca ggcgcaggcg cgaccggagc
      781 cgccggatcc ggcggtcgag caggcgctgc gcagcgagct ggcccaggcg cgcgacgagg
      841 cggtcgagct gcgcgacgcg ctcgcgcgcc tgcgccgggc cgccgagtcc gcggccgcga
      901 ccggcacgac cggcggcgcc gaggtcctgc gccgccagga ggtcctcaac aagctcgtcg
      961 atcacttctc ggtcttcgag atcgagatcg acggcaggct cgtcgacggc gtgatccaca
     1021 accgctgttg ctatcgcacg gagccgatga gcgagcagtg gctgccctgc ggcgaggcgc
     1081 cggcggccac caccgagctg cgcgactggc tggcggaggg cggtggcggc ctggtcgacg
     1141 cgctgcgccg gaccaagggc ggcaacgcga tgacgatcgt gcgacaaaac gagctcgcta
     1201 cctatcgcat cggccgcaag ctcgaggagc agctacgcga gcggttcccc gagcacaaga
     1261 tctacgacga gggcgtcgcc acgcactcgc tggcgtgctg cgattagaac gatgtgcggt
     1321 catccgccgc ggcgacgggc ctgggcggat gtatatcgct tggccctggc ctcggagagc
     1381 accggcggat ggaggtccga ggctttgatg aaacaccagc ggacgcctcg ggcccaggcc
     1441 atgtaatagc cgctggtatt gagataacgg acgccgacgg gaccgaggac cgggtgagat
     1501 tcgccgcggc cgccggagtt cttgtaggcg gtgatgacat cggccgcgcg gcgggtgggc
     1561 acgggtgagt tcttgcggcg ggtgccgggc ttgtgctcgc ggcgctgctc ctcggtcgga
     1621 cggaagcggc cgtagttgac ccacaggaac tggatcgacc ggtagacgcg gtcgttccag
     1681 cgcttgtaac tgatgggcgg cttcttgtag gggggctcgt ggccgcggcg aaacggctcg
     1741 aggttggcgc gcagcaccca gccgcaccgg ctgtgagcgg cgtagctgag gtgaggcgtg
     1801 ccgccggggc cggaggtcgc gtatccccac acccacttgc gcttgcggtg cttgtgggag
     1861 gcgtggaaga tcgtgccctc ctcgagggtg ccgaggcgcc acagcttctc ggtgcggatc
     1921 gagacggagc ggtcggcgtc ggggccgtcg tggtcgtcct tggcccgacg gacgatgtag
     1981 ctgcggccgg agcgcttgcg gacgcgggtg tggttgcctt cggaggaggt gtgcccggtc
     2041 gccatcgtca gtcttcctcc ctctcgtccg cgtcgggacc gaagtcgtcg tcgacctccg
     2101 tgtcgtcgtc gtcgtcgtcg tcgtcgtcgt catcctcgtc gtcggggtgc agcaggttga
     2161 ggccgtcgtc gtcgtcgtcg atcagctcga ggtgcagctc ctcgacctcg tgggtgccga
     2221 tccgccgggt gtcgccgttc tcgtcggtct cgccggtgac gttgatcccg ctggcgctgg
     2281 tgatgcgata cttgcggccg cgcaggggct cgccggagtg ctcgtcgagc actcgaaagc
     2341 cgccgtggta cgcgaagccg tcgtcgtcgg cgccaccgcg ggcgcgcagg gtgacctcga
     2401 cctcaccctt ctcggggatg acgacgcgct ggcgcccgac cgactggccc gcgaccgtaa
     2461 tcgtcgcgtg cgtgacaccg gcgggcaggt cgtgaaaggt cgcgacgtgc tggcgcctgg
     2521 gattgacgac ccggtgcacc ctgttgtcga gcagcgcgat gcgggcgagc ctcggcggcg
     2581 gcgcgccatc ggggaactcg accttgatcc gcacggtatt gaatgtaggg atcctgggcg
     2641 ccacgtagat ctcgccgcgc cacgatatca ccgcaccgat caccacgtca gctgtctgcc
     2701 agcaccccca cgcgcgggcg cgtcactgca tgtgcgtgct ttcgagcaaa cgcaaatcgg
     2761 ccctcgactg cgggagcagg cgagggccgg cggtgcgagc ggcgatcggt gatcagccgg
     2821 gcacgatcgg gacgcagcag ccggtctggc agtaggtgcc ctcggcgcag tcggagttgc
     2881 cctgctggtc gaaggtgcac gcgatgccgc cctcgcaggt cggatcgtcg catccgggcg
     2941 gcagctcgcc gccgaagcag acctcgcagc ccatcgggtc gcactcgtcg tcgcagtcgt
     3001 cgttcttggt gcagacctcg caatcctcga tcttgtcggc ggagcaggtc tggtcgccga
     3061 tgaagatgtc gtgcgagacg ccgccgatga cgatcgtgca gcaaccgaag cagtcgcagc
     3121 cgttgggcgt gccgcaggtc tcggcgcact ccggggactg cggctcgcac atcgccttcg
     3181 ggtcgtaggg acacttgtcg ccgccggggc tcagggggtc gcaggcgagg ttgaagtcg
//
LOCUS       DASWNJ010000520         3968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_186181, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000520 DASWNJ010000000
VERSION     DASWNJ010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186181"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VGB85_08805"
     CDS             complement(<1..887)
                     /locus_tag="VGB85_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134167.1"
                     /translation="MHRLSLAAFAVLAGCHMPEYKIFQEQQEKFASNGSGSSGEQAAT
                     TDDAGPESTSTSEGGMSPTATADSTGPTPPTNTATDTGDATDTADRPPVGEAERPVIV
                     SVALPDDIYTAGPVPLEIQTEHTLSVRVTLDGADAGQLLTSGGGLFTGELPVRGAIDN
                     GSHAIEVIATQGKYEDRRPDSYNVKTPKPGAEAWTMDGPPGSRTNRVAVAPAGHLIEV
                     GQTEIDGVPYPTLTMRSSSTGAELGPTKVLDTREGTVVDVAVLADGRMWVAMNVREPG
                     KDSRARIALLDADGNATGI"
     gene            complement(950..1894)
                     /locus_tag="VGB85_08810"
     CDS             complement(950..1894)
                     /locus_tag="VGB85_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134168.1"
                     /translation="MQPRSTLAPITLSLALILTACGGGDAKKTEAKKTETKAAETPAA
                     PAEDTKRDPGTSLDKAATTIDTSGPVPPEASAVFYTVDGALIPLGCYLHDKKKLGSGK
                     DCLKAVQQGDEVLLKSNTSETLDKIGAPKAAMCEGPASGAPTSLSAPAVDAGATYDFA
                     VAPKAFARQVVLLSEESWSEKKPGLSADETAALTALAKVQGELTIAQVALQDLDGDTV
                     PEKIVSVAQVNPKDSERYNFSGVFVQRGTAPGTWIPIETKSNDTETFRVRAALDLDGD
                     RSHELWLNSVTTEGGGGDRIYQLTQTGATGLGKWTCGL"
     gene            2018..2674
                     /locus_tag="VGB85_08815"
     CDS             2018..2674
                     /locus_tag="VGB85_08815"
                     /inference="COORDINATES: protein motif:HMM:NF024802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0134169.1"
                     /translation="MRATRPLSSELELVIGNMNLSSWSLRPWLALRATGHPFRERKIL
                     LDRPDSREKLRAVSPTGKVPVLIAGDIMIWESLAICEALAEWFPEAKLWPEDAGVRAV
                     ARSAAYEMHGGFPDLRREMPMNLRARIERAPSAAVQADIDRIVALWSDCRTRFGAGGP
                     YLFGEFSIVDCMYAPVVTRFRTYGVELDAGAQAYCEAMLARPDMRAWYAGAALETDAD
                     "
     gene            complement(2713..>3968)
                     /locus_tag="VGB85_08820"
     CDS             complement(2713..>3968)
                     /locus_tag="VGB85_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0134170.1"
                     /translation="PPRQDVPMTEPMTPVDPLDDWTSPAMLDDPHAIFHRLREQDPVH
                     WNSQTHCWVLTRHADVAAAVQDRRISSVPGGALRFSRQLEAEEMAHIGAVVPYLMMFM
                     QGMDPPAHTRQRALVQKAFTPRTLERLRPRVEQLVGEALDRAAESGTIELMSALAIPL
                     PATIIMEMLGVPAEGHGPVRRAAETIAEFLTLVNPAPGQLAAIAGRLNEFAAYITPLI
                     ASRRAEPQDDLLSALCTAEHDGEHLGEPELIVLTTMLLFAGHETTSNLIGNGAWALAS
                     HPEQWAALRDDPALVGPAVEEMLRHDAPVQVVPRWVHEAFELGGKQLEVGQRLLLNFA
                     AANRDPAFVADPDRFDIRRPATKHLAFGHGIHHCLGVALARIEAQAVFAALARRFHTL
                     ELDPERPPQRKPNVVLRGPAHLHLR"
BASE COUNT          486 a         1219 c         1592 g          671 t
ORIGIN      
        1 tcgatcccgg tcgcgttacc gtccgcgtcc agcaaggcga tccgcgcgcg tgagtccttc
       61 cctggttcgc gcacgttcat tgcgacccac atgcggccgt ccgcgaggac cgcgacgtcg
      121 accacggtgc cctcgcgtgt gtcgagcacc ttcgtgggcc ccaactcggc gccggtcgag
      181 ctcgagcgca tggtcagcgt cgggtagggt acgccgtcga tctcggtctg gccgacttcg
      241 atcaggtgcc cggcaggtgc gaccgcgacg cggttggtgc ggctccccgg gggtccgtcc
      301 atcgtccagg cctcagcgcc gggcttggga gtcttgacgt tgtaggaatc ggggcggcgg
      361 tcctcgtact tgccttgcgt ggcgatgacc tcgatcgcgt gcgagccgtt gtcgatcgcg
      421 ccgcgcactg gcaactcgcc ggtgaacaat ccaccgcccg acgtgagtaa ttggcctgcg
      481 tcggcgccgt cgagcgtcac gcgcaccgat aaagtatgct cggtctgtat ttcgaggggt
      541 acagggcccg ccgtgtagat atcgtctggc agcgccaccg agacgatgac aggtcgctcg
      601 gcttcgccga ccggcgggcg gtcggccgtg tcggtcgcgt cgccggtgtc ggtcgcggtg
      661 ttcgtcggtg gggttggccc cgtgctgtcg gccgtggcgg tcggggacat gccgccctcg
      721 ctcgtgctcg tgctctcggg cccagcgtcg tcggtcgtgg ccgcctgttc gccgctcgag
      781 ccgctgccgt tacttgcgaa cttctcctgt tgttcttgga agatcttgta ctcgggcatg
      841 tgacagcccg cgaggacggc gaaggcggcg agggacaggc ggtgcatacc cgcgcatggt
      901 accaggtgta gcgaatccgc gcctccttga aggcttgtag tcggtcgggc tacaggccgc
      961 aggtccactt gccgaggccg gtggcgccgg tctgggtgag ctggtagatg cggtcgccgc
     1021 cgccgccctc ggtggtgacg gagttgagcc acagctcgtg gctgcggtca ccgtcgaggt
     1081 cgagggccgc gcggacgcgg aaggtctcgg tgtcgttgga cttggtctcg atcgggatcc
     1141 aggtgccggg ggcggtgccg cgctggacga acacgccgct gaagttgtag cgctcgctgt
     1201 ccttggggtt gacctgggcg acggacacga tcttctcggg cacggtgtca ccgtcgaggt
     1261 cctgcagggc gacctgggcg atcgtcagct cgccctggac cttggcgagg gcggtgaggg
     1321 cggcggtctc gtcggcggag agcccgggct tcttctcgct ccagctctcc tcggagagga
     1381 ggacgacctg gcgggcgaag gccttggggg cgaccgcgaa gtcgtaggtc gcgccggcgt
     1441 cgacggcggg ggcggacagg ctggtgggcg cgccggaggc cgggccctcg cacatggcgg
     1501 ccttgggggc gccgatcttg tcgagggtct cgctggtgtt cgacttgagg aggacctcgt
     1561 cgccctgctg gacggccttg aggcagtcct tgccgctgcc gagcttcttc ttgtcgtgca
     1621 ggtaacagcc gagcgggatc agggcgccgt cgacggtgta gaacacggcg ctggcctcgg
     1681 gcgggaccgg gccgctggtg tcgatggtgg tcgcggcctt gtcgaggctg gtgccggggt
     1741 cgcgcttggt gtcctcggcc ggcgcggccg gggtctcggc ggccttggtc tcggtcttct
     1801 tggcctcggt cttcttggcg tcgccgcccc cgcaggcagt gagaatgagg gcgagcgaga
     1861 gcgtgatcgg ggccagggtc gagcggggct gcatggggcg gccaccatag caacggcggg
     1921 cagggcctgg ccagttccgg acctggaacg gggttgcggg gcggccccgg ctctggaggc
     1981 gtggtgcgcg cgggcgcgcc gggatacgct ggtcggcatg cgcgccaccc gtccgctatc
     2041 ttccgagctc gagctcgtga tcggcaacat gaacctctcg tcgtggagct tgcggccgtg
     2101 gctggccttg cgggcgacgg gtcatccgtt ccgcgagcgc aagatcctgc tcgaccggcc
     2161 agattcgcgg gagaagttgc gggcggtctc gccgaccggg aaggtgccgg tgctgatcgc
     2221 cggcgacatc atgatctggg agtcgctggc gatctgcgag gcgctggcgg agtggttccc
     2281 ggaggcgaag ctgtggcccg aggacgcggg cgtgcgagcg gtcgctcgca gcgcggcgta
     2341 cgagatgcac ggtggttttc ctgacctgcg gcgcgagatg ccgatgaacc tgcgggcgcg
     2401 catcgagcgg gcgccgtcgg ccgcggtgca ggcggacatc gaccgcatcg tcgcgctgtg
     2461 gagcgactgc cggacgcgct tcggcgccgg tgggccgtac ctgttcggcg agttctcgat
     2521 cgtcgactgt atgtacgcgc cggtggtgac ccggtttcgt acctacgggg tcgagctcga
     2581 cgcgggcgcg caggcgtact gcgaggcgat gctggcgcgg ccggacatgc gggcgtggta
     2641 cgccggcgcg gcgctcgaga ccgacgcgga ctaaaacctg tccgtgagga catgccccgt
     2701 ggggcatgtc ctttagcgca ggtggaggtg ggccgggccg cgcagcacga cgttgggttt
     2761 gcgctggggc gggcgctccg ggtcgagctc gagggtgtgg aagcgccggg cgagggcggc
     2821 gaacacggcc tgggcctcga tgcgggcgag ggcgacgccg aggcagtggt ggatgccgtg
     2881 gccgaaggcg aggtgtttgg tggccgggcg gcggatgtcg aagcggtcgg ggtcggcgac
     2941 gaacgcgggg tcgcggttgg cggcggcgaa gttgaggagc aggcgctggc cgacctcgag
     3001 ctgcttgccg ccgagctcga aggcctcgtg gacccaccgc ggaacgacct ggaccggcgc
     3061 gtcgtggcgg agcatctcct cgacggcggg gccgacgagc gcagggtcgt cgcgcagggc
     3121 ggcccactgc tcggggtggc tggcgagggc ccacgcgccg ttgccgatca ggttgctggt
     3181 ggtctcgtgg ccggcgaaca gcagcatggt ggtcagcacg atcagctcgg gctcgccgag
     3241 gtgctcgccg tcgtgctcgg cggtacacag cgcggacagc aggtcgtcct gcggctcggc
     3301 gcggcgcgag gcgatcagcg gggtgatgta cgcggcgaac tcgttgaggc gcccggcgat
     3361 cgcggcgagc tggccggggg cggggttcac gagggtgaga aactcggcga tcgtctcggc
     3421 ggcgcggcgg accgggccgt ggccctcggc cggtacaccg agcatctcca tgatgatcgt
     3481 cgcgggcagc gggatcgcca gcgcggacat cagctcgatc gtgccggact cggcggcgcg
     3541 gtcgagggcc tcgcccacga gctgctcgac gcgcgggcgc aatctctcga gcgtgcgcgg
     3601 cgtgaaggcc ttctggacca gcgcgcgctg gcgggtgtgc gccggcgggt ccatgccctg
     3661 catgaacatc atcaggtagg ggacgacggc gccgatgtgg gccatctcct ccgcttcgag
     3721 ctgccgcgag aagcgcagcg cgccgccggg gaccgagctg atgcgacggt cctggacggc
     3781 cgcggcgacg tcggcgtggc gcgtgaggac ccagcagtgg gtctgcgagt tccagtgcac
     3841 gggatcttgc tcgcggaggc ggtggaagat cgcgtggggg tcgtcgagca tcgcgggcga
     3901 cgtccagtcg tcgagggggt ccaccggggt catcggctcg gtcatgggta cgtcctgtcg
     3961 agggggcc
//
LOCUS       DASWNJ010000521         1652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_221420, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000521 DASWNJ010000000
VERSION     DASWNJ010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221420"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="VGB85_08825"
     CDS             <1..851
                     /locus_tag="VGB85_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HEY0134171.1"
                     /translation="LLKVNMQYIASAAMDDKYRTEPPFKLQGSYRNMNKMAEKIVAAM
                     TPEEVDRLVDDHYQGEAQTLTIAAEQNLLKLAEMRGRLNPTQAERWATIKREFQRTRS
                     LGGAEDDPAVRIGGQLSILAQRVESITDALKTAASRGPSEEAEEAARAQQTRMMEMMA
                     LMQQQVAAAREKPAEKPPEKALAPPTIAAGPLEWARPQIARIANALETLGQSSLAIEV
                     KNEAPPGITELLAQQVAIIERTLVPLVRAATGNLQDMKSLELRVDQMLGMLQEIDEGL
                     KAKFAR"
     gene            complement(855..>1652)
                     /locus_tag="VGB85_08830"
     CDS             complement(855..>1652)
                     /locus_tag="VGB85_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134172.1"
                     /translation="TTGPGEPTTAGDESTGAPGSTPVSVPDSEGEVTTGSATTTAPTD
                     TESIPAFMPMPCDTWNDECPAGQHCIPYGDDGAPYPGRMGCFPLVPEPLPVGEVCTPL
                     AIEQGTVVTPDPCERGLVCSWRTGTCLALCQGSLDAWTCPTLSTCTGWEEEPLCWPIC
                     DPLEPTCPPGEICNVDTTVGFQCRPASPDDLPLFSPCSGGGECGVGTFCDWAAPAVEC
                     DPAFERCCNKICDLDGDTCPGVGQTCVPFDYYVPLPDYADLGRCAVP"
BASE COUNT          282 a          560 c          593 g          217 t
ORIGIN      
        1 cgctgctaaa ggtcaatatg cagtacatcg cgtcggcggc gatggacgac aagtaccgca
       61 ccgagccgcc gttcaagctc cagggcagct atcgaaacat gaacaagatg gccgagaaga
      121 tcgtcgcggc catgaccccc gaggaggtcg accgcctcgt cgacgatcat tatcagggcg
      181 aggcgcagac cctgacgatc gcggcggagc agaacctgct caagctcgcc gagatgcgcg
      241 gccgcctcaa cccgacccag gccgagcgct gggcgacgat caagcgcgag ttccagcgca
      301 cccgtagcct cggcggcgcc gaggacgacc cggcggtgcg catcggcggc cagctgagca
      361 tcctggccca gcgcgtcgag agcatcaccg acgcgctcaa gacagccgcc agccgcgggc
      421 cctcggagga ggccgaggag gcggcgaggg cccagcagac ccgcatgatg gagatgatgg
      481 cgctgatgca gcagcaggtc gccgcggcgc gcgagaagcc ggccgagaag ccgccggaga
      541 aggccctggc gccgccgacg atcgccgccg gtccgctcga gtgggcgcgc ccgcagatcg
      601 cccgcatcgc caacgcgctc gagaccctcg ggcagagctc gctggcgatc gaggtcaaga
      661 acgaggcgcc tccgggcatc accgagctgc tcgcgcagca ggtggcgatc atcgagcgca
      721 cgctggtccc gctagtgcgc gcggcgaccg gcaacctgca ggacatgaag agcctcgagc
      781 tgcgcgtcga tcagatgctc ggcatgctgc aggagatcga cgagggttta aaagcgaagt
      841 tcgcgcgctg agcttcacgg cacggcgcag cggccgaggt cggcgtagtc ggggagcggc
      901 acgtagtaat cgaagggcac gcaggtctga ccgacccccg ggcaggtgtc gccgtcgagg
      961 tcgcagatct tgttgcagca gcgctcgaag gcagggtcgc attcaaccgc cggtgcggcc
     1021 cagtcgcaga aggtgccgac gccgcattca ccacccccgc tgcacggtga gaacagcggc
     1081 aggtcgtcgg ggctggcggg gcgacactgg aagccgaccg tggtgtccac gttgcagatc
     1141 tcgccgggcg ggcaggtcgg ctcgagcggg tcgcagatcg gccagcacag cggctcttcc
     1201 tcccagcccg tgcaggtcga cagcgtcggg caggtccacg cgtcgaggct gccctggcac
     1261 agcgcgaggc aggtgcctgt ccgccacgag cacacgaggc cgcgctcgca cgggtcgggc
     1321 gtgacgacgg tcccttgctc gatcgccagc ggcgtgcaga cctcgccgac gggcagcggc
     1381 tccggcacga gcgggaagca tcccatccga cccggatatg gcgcgccatc atctccgtag
     1441 gggatgcaat gctggcccgc ggggcactcg tcgttccagg tgtcgcaggg catcggcatg
     1501 aacgccggta tcgactcggt gtccgtggga gccgttgtcg tcgcgctccc ggtcgtcacc
     1561 tcgccctcgc tgtcggggac gctcacgggc gtcgaccctg gcgctccggt ggattcgtct
     1621 ccggcggtcg tcggctcgcc gggcccggtc gt
//
LOCUS       DASWNJ010000522         4473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_115332, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000522 DASWNJ010000000
VERSION     DASWNJ010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115332"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="VGB85_08835"
     CDS             complement(<1..1096)
                     /locus_tag="VGB85_08835"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEY0134173.1"
                     /translation="MARPLPQIASFLLALIAAYAGLATAGPVAPGELPALSAAFPVEA
                     VPSDRPTATAAPAPELVDWPAEIAGRLAALREPPERRGHPRPADAAPPDDPTLAALHQ
                     LQQRLAAVVRRTGGARIGVHVRDLDSGRTLFDHHGNERYNPASNQKLVTAIAAVELLG
                     PGYRFRTRVLRRDDTLVLVGEGDPSLQLTDLHALASEVIAAGAHRGIRRIVVDDSAFS
                     PERHGPGYSPQGDGASYTAPSGALSLAFNTAVVTIRPTTPGEPASIDLSPPSAHLRLQ
                     GEVRTGRGRPLRVASRDADDLTVIEVDGALPAGHAPVTIRRRIADPGLFTGHAFADVL
                     RQLAEAQDQESIALPVLREPAGPNAREVATH"
     gene            complement(1187..1381)
                     /locus_tag="VGB85_08840"
     CDS             complement(1187..1381)
                     /locus_tag="VGB85_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134174.1"
                     /translation="MGYGDNRRTPKMTRKKRQTKLQLRIKRRAVEVRAARQSKNPQQA
                     AEPAKKSKAKKEKAPKAAQA"
     gene            1593..2957
                     /gene="serS"
                     /locus_tag="VGB85_08845"
     CDS             1593..2957
                     /gene="serS"
                     /locus_tag="VGB85_08845"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY0134175.1"
                     /translation="MLDIKLLRDDSQKIAQNLADRHARVFADLGPADGPDWATRTTDR
                     LVALDRRYLDLLQKQDELRRAQNETSAGMKAVGKLPKEEQAAAREKLVEEGRRLRDEE
                     RQLTLDTEAALQERDETWSRVPNLTHPETPRGATDDDHRELRRVGEPRDFAAEGFTPA
                     DHLAVAEALELVDFASGAKVTGPKFYYLKNDAVLLDLALQHFALGVARRHGFTLHTTP
                     DLAREEILRGLGFNPRGESTQIYSIADSDLCLVGTAEITLGGMLADTILEEDQLPLLL
                     AGVSHCFRTEAGSAGQESRGLYRVHQFTKVELFAFVSAEDDASEVMHARILAIEEEIF
                     QALGIPYRVLDIASGDLGGPAYRKFDLEAWMPGRGAYGEITSTSNCTDYQARRLKIRY
                     RPSAPDGSKQKPRHVHMLNGTAVACSRALVAILENYQQKDGTVLVPEVLRPIVGKDRI
                     TSRA"
     gene            2996..3652
                     /locus_tag="VGB85_08850"
     CDS             2996..3652
                     /locus_tag="VGB85_08850"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEY0134176.1"
                     /translation="MMILAHRGASDGRLENTLPAFAHAIERGADGVELDVQLSRDGEV
                     VVFHDEDLVRLAGRHARIEHLDWDELERVVLVRGAKIPRFSELLAMWPTDRWLNVELK
                     AGGAQVARETVRLLAGRPRVILSSFDPRMLLAARAAGSGYEHALLLAAESPPFLHVKG
                     AQAFGCAAVHLDHRLCTASTIQRYREQGLQVGVWTVNDVQRKQVVAGWGVDRIITDSP
                     "
     gene            complement(3675..>4473)
                     /locus_tag="VGB85_08855"
     CDS             complement(3675..>4473)
                     /locus_tag="VGB85_08855"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEY0134177.1"
                     /translation="LKASGWQGGFGIGAIWNWQQRLWIGASYTSQPNVVGGMTLKGTL
                     NNALATSAVESPGAELTQTFPDIIRLGFRVRPSDKFELRLFGDYTRWSVFDKQCVLNA
                     SNPDRKCDFPGADTALSKPEEFGKDVPDPDDGGPKTSPTAGVIQHLPRFWKDAGGVRL
                     GASYWFVPQLEAYAGLGFDSSAVRPATLDAALMDMNKMSASIGARWQIIRQFALALTI
                     TDIAYFKVDTRGKSILNKFQPPTRQPKADGVYKQNILLANLFVDVSF"
BASE COUNT          649 a         1505 c         1674 g          645 t
ORIGIN      
        1 ggtgcgtggc gacctcgcgg gcattgggac ccgcgggctc gcgcaggacc ggcagcgcga
       61 tcgactcctg gtcctgcgcc tcggcgagct gacgcaggac atcggcgaag gcgtggccgg
      121 tgaacaggcc gggatcggcg atccggcgcc ggatcgtgac cggggcgtgg ccggcgggca
      181 gggcaccgtc gacctcgatc acggtcaggt cgtcggcgtc gcggctggcg acccgcagcg
      241 gccggccgcg gccggtgcgg acctcgccct gcaggcgcag gtgggcgctc ggcgggctga
      301 ggtcgatgct cgcgggctcg cccggtgtcg tcgggcggat cgtcaccacg gccgtgttga
      361 aggcgaggga cagcgcgccg ctcggggcgg tgtagctggc gccgtcgccc tgcgggctgt
      421 aaccggggcc gtggcgctcg ggggagaagg cgctgtcgtc gacgacgatc cgccggatcc
      481 cacggtgcgc gccggcggcg atcacctcgc tggcgagcgc gtgcaggtcg gtcaattgca
      541 ggctcgggtc gccctcgccg acgagcacga gggtgtcgtc gcggcgcagc acccgggtgc
      601 gaaaccggta gcccggaccg agcagctcga ccgctgcgat cgcggtgacg agcttctggt
      661 tcgaggcggg gttgtagcgc tcgttgccgt ggtggtcgaa cagggtacgg ccgctgtcga
      721 ggtcacgcac gtgcacgccg atccgcgcgc caccggtgcg acgcacgacg gcggcgagac
      781 gctgctgtaa ttggtgcagc gccgcgaggg tcgggtcgtc cggcggcgcg gcgtcggccg
      841 gccgcggatg accacgtcgc tcaggcggct cacgcagcgc ggcgaggcga ccggcgatct
      901 cggcgggcca gtcgacgagc tcgggcgcag gagcagcggt cgcggtcggc ctgtccgaag
      961 ggacagcctc gaccgggaac gcggcggaca gcgccggcag ctcgcccggg gccaccggac
     1021 cggcggtcgc gaggcccgcg tatgcggcga tcagggcgag gagaaagctg gcgatctgcg
     1081 ggagcgggcg ggccacggcg cgagcctcga gggaacgaag agtcccatcg atgtaccccg
     1141 cgcccggagc ccgacaaaat taccggcgct ccggttcgat cgcggctcag gcctgcgcgg
     1201 ccttgggggc cttttccttc ttggccttgc tcttcttggc gggctcggcg gcctgctggg
     1261 ggttcttgga ctgccgcgca gcccggacct cgaccgcgcg ccgcttgatg cggagctgca
     1321 gcttggtctg gcgcttcttg cgggtcatct tcggcgtgcg gcggttatct ccgtatccca
     1381 tggcgaaact cgtgtcttga aggaggaaag gaccccttgg ggggtccggg cgcgggagca
     1441 tggacgagcg cgcccccggg gtcaaggccc gcggcgaggt ccggacgcgg tcgtcggcgc
     1501 tgcgaggcag gccggtatcg ccgggtattg ccgcttgttg ccgcgtgttg ccgcgtgttg
     1561 ctgcctccag ggcctgttat cctccgcgcg ccatgctcga catcaagctg ctccgcgacg
     1621 acagccagaa gatcgcccag aacctcgccg accgtcatgc gcgtgtgttt gcggacctgg
     1681 gacccgcgga cgggcccgac tgggcgaccc gcacgacgga tcgcctggtc gcgctcgacc
     1741 gccgctacct cgacctcctg cagaagcagg acgagctgcg ccgggcccag aacgagacca
     1801 gcgccggcat gaaggcggtc ggcaagctgc cgaaagagga gcaggccgcg gcgcgtgaga
     1861 agctcgtcga ggaggggcgc cggctgcgcg acgaggagcg ccagctgacg ctcgacaccg
     1921 aggccgcgtt gcaggagcgc gacgagacct ggagccgcgt gcccaacctg acccacccgg
     1981 agacgccgcg cggcgcgacc gacgacgacc atcgcgagct gcgccgcgtc ggcgagccgc
     2041 gagacttcgc ggccgagggc ttcacgccgg ccgaccacct cgcggtcgcc gaggcgctcg
     2101 agctggtcga cttcgccagc ggggccaagg tcaccggccc gaagttttat tatctcaaga
     2161 acgacgcggt gctgctggac ctcgcgttgc agcacttcgc gctgggcgtc gcgcggaggc
     2221 acgggttcac gctgcacacc acgccggacc tcgcgcgcga ggagatcctt cgcggcctcg
     2281 ggttcaaccc gcgcggcgag tcgacccaga tctacagcat cgccgacagc gacctgtgcc
     2341 tcgtcggcac cgccgagatc acgctcggcg gcatgctggc cgacacgatc ctcgaggagg
     2401 accagctgcc gctgctgctc gccggcgtgt cgcactgctt tcgcaccgag gccggctcgg
     2461 ccgggcagga gtcgcgcggc ctgtatcgcg tgcatcagtt caccaaggtc gagctgttcg
     2521 cgttcgtctc ggccgaggac gacgcgagcg aggtgatgca cgcgcggatc ctggcgatcg
     2581 aagaggagat ctttcaggcc ctggggatcc cgtaccgcgt gctggacatc gccagcggcg
     2641 acctcggcgg accggcgtac cgcaagttcg acctcgaggc gtggatgccc ggccgcggcg
     2701 cctacggcga gatcacgagc acgagcaact gcaccgacta ccaggcgcgc cgcctgaaga
     2761 tccgctatcg cccgtccgcg ccggacggca gcaagcaaaa gccgaggcac gtgcacatgc
     2821 tcaacggcac cgcggtcgcc tgctcgcgcg cgctggtggc gatcctcgag aactaccaac
     2881 aaaaggacgg caccgtgctc gtgccggagg tcctgcggcc gatcgtcggc aaggaccgca
     2941 tcaccagccg ggcgtagcgc cgcagcggca gatctgtggc ataagaggcc ccgtcatgat
     3001 gatcctcgcg caccgcggcg ccagcgacgg ccgcctggag aacaccctgc cggccttcgc
     3061 ccacgcgatc gagcgcggcg ccgacggggt cgagctcgat gtccagctca gccgcgacgg
     3121 cgaggtcgtc gtgttccacg acgaggacct ggtgcgcctc gccggccggc acgcgcgcat
     3181 cgagcacctc gactgggacg agctcgagcg cgtggtcctc gtgcgcggcg cgaagatccc
     3241 ccgcttctcc gagctgctgg cgatgtggcc gacggaccgc tggctcaacg tcgagctcaa
     3301 ggccggcggg gcgcaggtcg cccgcgagac ggtgcgcctg ctcgccgggc gcccgcgcgt
     3361 gatcctcagc agcttcgatc ctcgcatgct gctcgcggcg cgcgcggccg gcagcggcta
     3421 cgagcacgcg ctgctgctcg cggccgagag cccgccgttt ttgcacgtca agggcgcgca
     3481 ggcgttcggc tgcgccgcgg tccacctcga ccaccgcctg tgcaccgcga gcacgatcca
     3541 gcgctaccgc gagcagggcc tgcaggtcgg cgtgtggacg gtcaacgacg tgcagcgcaa
     3601 gcaggtcgtc gccggctggg gcgtcgaccg catcatcacc gatagcccat gacggggcgg
     3661 acgggcgtct cgcgtcagaa cgagacgtcg acgaagaggt tggcgagcag gatgttctgc
     3721 ttgtagacgc cgtcggcctt gggctggcgg gtcggcggct ggaacttgtt gaggatgctc
     3781 ttgccccggg tgtcgacctt gaaatacgcg atgtcggtga tcgtcagcgc gagcgcgaac
     3841 tggcggatga tctgccagcg ggcgccgatg ctggcggaca tcttgttcat gtccatcagc
     3901 gcggcgtcga gggtcgccgg gcgtacggcg ctgctgtcga agccgaggcc ggcgtaggcc
     3961 tcgagctgcg ggacgaacca gtacgaggcg ccgaggcgca caccgccggc gtccttccag
     4021 aagcgcggca ggtgctggat gacgcccgcg gtcggcgatg tcttcggacc gccgtcatcg
     4081 gggtcgggca cgtccttgcc gaattcttcg ggcttgctca gggccgtgtc ggcgcccggg
     4141 aagtcgcact tgcggtcggg gttggaggca ttgaggacgc actgcttgtc gaacaccgac
     4201 cagcgggtgt agtcgccgaa caggcgcaac tcgaacttgt cgctcgggcg cacgcgaaag
     4261 ccgaggcgaa tgatgtccgg aaaggtctgg gtcagctcgg cgccgggtga ctcgaccgcg
     4321 ctggtggcga gggcgttgtt gagggtgccc ttgagggtca tgccgccgac gacgttgggc
     4381 tggctggtat aggacgcgcc gatccacagg cgctgctgcc agttccagat cgcgccgatg
     4441 ccgaagccgc cctgccagcc ggaggccttg agc
//
LOCUS       DASWNJ010000523         6518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_468483, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000523 DASWNJ010000000
VERSION     DASWNJ010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468483"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2449
                     /locus_tag="VGB85_08860"
     CDS             <1..2449
                     /locus_tag="VGB85_08860"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0134178.1"
                     /translation="LTQALEINPGYVPALISLTELYKNRRDWLKAARNLEAATEYSTF
                     KLEKTNLAAEAGFIYYEELDKKDKAVALFAKVLELDPEHVKVGRVLGQIYYDDGNFAG
                     ADPIYDVLTRKTEQLGLSEQDQRDLFLRAAKVARALGNSDKALKQYRKAYDLDSTNRD
                     VLVGMADLLFEKEDWEKAFKLYQTILVQHRETQSSEETVLVYYRLGMIKKRQNEPRKA
                     LNYLEKALEVDAHDLNTLNAVIDLQTAANDWEGVISAKRAMIDVADGEKQADIYKEIG
                     KLYLEKLANWQKSAAAYSSALDLQPKDYPLLHTLLDIYTKNKQWEDSIRIIDRIVEIE
                     TDPKRKSRYNYTAAVLLRDEIKAHDEAIDRFNMVLDDDPSFLKAFQAIDTMVTKSKDW
                     KTLERSYRKMLKRLPQNDQSDLKVTLWSNLAEIYRTRLKDFKAAAAAFEVAAKLDPTN
                     VERHFLLAELYEALSADNPTEFATQAVKEHQILIAQEPFRYASYHALFNIYFKSKQLD
                     KAFCVARTLAFLKQATPEEQGVYEKYNVAEFQQARQRLSEETLRRHVFHPDEDLFITG
                     ILGSIAPALAAWRANPLPAYLKPNERTDINSDPSLVARMAKYVMNVLNVGQPDLYLRP
                     DEPGDVALLNAVRDNRVAPTMVLFSNLLKGKNEKHLAFALGRHMLDLYLPHYAYVALD
                     RSPQNLKQIFLTCMHVCDMETGLPKAELDGYAREIFSRLPGGARDQMRSLMRKFVEAG
                     GSTDVKKWALATEVAGYRVGFLLCNDMVVAAHIISQEQSAFGSSMTPKDKIKELVLYS
                     ISEDYFKARKGIGIAVA"
     gene            complement(2684..4297)
                     /locus_tag="VGB85_08865"
     CDS             complement(2684..4297)
                     /locus_tag="VGB85_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY0134179.1"
                     /translation="MAAKLILLGGDHPVEHVLSSFNTLGRHPDNSIQVLDRIVSKEHS
                     RITLGPDGRYVVRDVGSLNGTFVNGEKVSERALKSGDQISLGNTTYRFTEDRPQDKLP
                     IRKVTMTPDQVQSQVQSTLSANSRFLPEKDIKDVAVLRADYEKLRVAHELSQKLSIDA
                     DLDTLLQKIVDEAFLLIRADRAVILLLDGETDDFIPRYVRQKRDEEIKLSKSILDEVR
                     TKKRAVLSSDAMVDERFKAAKSIIMQGIRSTMCVPLLYNDKLLGAMHMDSMLATGAFT
                     EKDLLLFSGIATQAAVAIENNMLAQKIEHEATARAQFQRLLSPNLVDQIVAGQLTLDQ
                     GGARRDVTMLFADIRGFTAMSERHTPEEMVVTLNEYFEVMVDVLFRHGGTLDKYVGDE
                     IIGLFGAPVDLMDAPLRSVRCALDMMRALEEFNRLRVDQGREKVSIGIGVNTGSVIAG
                     AIGSSRTLQYTVIGDAVNIAARLCSLAKPGEIIISESTLELAGPHILVEPRKPVQVKG
                     KREALPIYAVQGIRDTAPPSSRSGSLMST"
     gene            4599..>6518
                     /locus_tag="VGB85_08870"
     CDS             4599..>6518
                     /locus_tag="VGB85_08870"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0134180.1"
                     /translation="MAVVSPQEPEGPQRLAAAGAGRVLVLGAPLAPSPGVEVWAGPFA
                     TPLRLPLRDGSVDALLCIEAYAALGPEDRAALVSEARRVLRATGVLAIYSADLSETEA
                     LVRANFSEVAALAQLPWSGFRLAPVGASPSSEVRLVEDLRTGEPPTGSYLVLASATEL
                     GPLVQQCVLVATTPEPASATPIAATNDLAVDETPPQPQPGEALSEAFSRLAAATTASP
                     SSQRVPPLDPIDEGDVLAGLFEAPAESESSPEATEPEPPPIDPAELAELREAVDTLRR
                     ERDSLRERAATLTQELRHAHAQAIDAGAELHELRRRVDTQRLEDGSPLLDDAGLRNDA
                     AALTRVLVDEVAPMVTPEATRPTADVAIDRDRLREELTRRTADLQELETRLWQSEEEV
                     QRERLENVRLVTDVDRLREQVDRSRIVEHERMQELERLGHELRRLELAYAELQGLYTT
                     GDHRLRELEASMERGDDPPELVELEAQVRELKGERDQAQALERAAVEQARRRERELAE
                     AGRTIRELRRSVEEHASVAANLRGELAVMQVEVEQFGAAVPALQERLRDHQRKILERE
                     EEAGELQRRLEESVAEQQELRQRLRRYKQDLEVAVNTKEAIEQDLDRLRGELDAKRRS
                     VEQLQQLVALGAGTGR"
BASE COUNT         1108 a         2255 c         2216 g          939 t
ORIGIN      
        1 gttgacccag gccctcgaga tcaaccccgg gtacgtgccc gccctcatca gcctcaccga
       61 gctctacaag aaccggcgtg actggctcaa ggcggcccgc aacctcgagg ctgcgaccga
      121 gtactcgacc ttcaagctcg agaagaccaa cctcgcggcc gaggcgggct tcatttacta
      181 cgaggagctc gacaagaagg acaaggcggt cgccctgttc gccaaggtcc tcgagctcga
      241 cccggagcac gtcaaggtcg gccgcgtgct cggccagatc tactacgacg acggcaactt
      301 cgccggcgcc gacccgatct acgacgtgct gacccgcaag acggagcagc tcgggctcag
      361 cgagcaggac cagcgcgatc tgttcctgcg cgccgccaag gtcgcccgcg cgctcggtaa
      421 ctccgacaag gcgctcaagc agtaccgcaa ggcctacgac ctcgactcga ccaaccgcga
      481 cgtgctcgtc ggcatggccg acctgctgtt cgagaaggag gactgggaga aggcgttcaa
      541 gctgtaccag acgatcctgg tccagcaccg cgagacccag agctccgagg agacggtgct
      601 cgtctactac cgcctcggca tgatcaagaa gcggcagaac gagccgcgca aggcgctcaa
      661 ctacctcgag aaggcgctcg aggtggacgc ccacgatctg aacaccctca acgcggtcat
      721 cgacctccag acggcggcga acgactggga gggcgtcatc tcggccaagc gggccatgat
      781 cgacgtggcg gacggcgaga agcaggccga catctacaag gagatcggca agctctacct
      841 ggagaagctc gccaactggc agaagtcggc ggcggcgtac tcgagcgccc tcgacctcca
      901 gcccaaggac tacccgctcc tccacacgct gctcgacatc tacaccaaga acaagcagtg
      961 ggaggattcg atccggatca tcgaccgcat cgtcgagatc gagacggacc cgaagcgcaa
     1021 gtctcgctac aactacaccg ccgcggtgct gctccgcgac gagatcaagg cccacgacga
     1081 ggcgatcgac cgcttcaaca tggtcctcga cgacgacccg agcttcctca aggccttcca
     1141 ggccatcgac acgatggtca ccaagtccaa ggactggaag accctcgagc gctcgtaccg
     1201 caagatgctc aagcggctgc cgcagaacga ccagtcggac ctcaaggtca cgctgtggag
     1261 caacctggcc gagatctatc ggacccggct caaggacttc aaggccgccg cggctgcctt
     1321 cgaggtcgcc gccaagctcg acccgaccaa cgtggaacgc cacttcctgc tcgcggagct
     1381 gtacgaggcg ctctcggccg acaacccgac cgagttcgcc acccaggccg tcaaggagca
     1441 ccagatcctg atcgcccagg agccgttccg ctacgcgtcg taccacgcgc tcttcaacat
     1501 ctatttcaag agcaagcagc tcgacaaggc gttctgcgtg gcgcgcacgc tggccttcct
     1561 caagcaggcc acgccggagg agcagggcgt ctacgagaag tacaacgtcg ccgagttcca
     1621 gcaggcgcgt cagcgcctca gcgaggagac gctgcgcagg cacgtcttcc atccggacga
     1681 ggacctgttc atcacgggca tcctcgggtc gatcgctccg gccctggcgg cctggcgggc
     1741 caacccgctc ccggcctacc tcaagccgaa cgagcgcacc gacatcaaca gcgacccgtc
     1801 gctggtggcc cgcatggcca agtacgtgat gaacgtgctc aacgtcggcc agccggacct
     1861 gtacctgcgt ccggacgagc cgggcgacgt ggccctgctc aacgccgtcc gcgacaaccg
     1921 cgtcgcgccg acgatggtgc tgttctccaa cctgctcaag ggcaagaacg agaagcacct
     1981 ggccttcgcg ctcggtcggc acatgctcga cctgtacctg ccgcactacg cctacgtggc
     2041 gctcgaccgc tcgccgcaga acctcaagca gatcttcctg acctgcatgc acgtctgcga
     2101 catggagacc ggcctgccga aggccgagct cgacgggtac gcccgcgaga tcttcagccg
     2161 cctgcccggc ggtgcgcgcg accagatgcg cagcctgatg cgcaagttcg tcgaggccgg
     2221 cggctcgacc gacgtgaaga agtgggccct cgcaacggag gtcgcgggct accgcgtcgg
     2281 cttcctgctc tgcaacgaca tggtcgtcgc ggcccacatc atctcgcagg agcagagcgc
     2341 cttcggctcg tcgatgaccc cgaaggacaa gatcaaggag ctcgtcctct acagcatcag
     2401 cgaggactac ttcaaggcgc gcaagggcat cggcatcgcc gtcgcctgag ccacttcaaa
     2461 ctcgaagcac gaggggcgac cgcgagagcg gccgcccttc gggcgtttaa gcgccgcacg
     2521 cgaccgcagc ggcccctcgc ggtacgcgga ggcgacggcc gcccgccgtc tgcgtgcgag
     2581 gccgcggccc tctcggggcg gcccgcgcag gccgacatca gcgcgccgct gcggcccgtc
     2641 cggccgacgt cggcgcgcca ctgcggccgc gctggcgggc cgctcaggtc gacatcagcg
     2701 agccgctgcg gctgctgggc ggggccgtgt cacggatgcc ctggaccgcg tagatcggca
     2761 gcgcctcgcg cttgcccttg acctgcaccg gcttgcgcgg ctcgaccaga atgtgcggtc
     2821 ctgcgagctc gagcgtcgat tcggagatga tgatctcgcc cggcttggcg aggctgcaca
     2881 ggcgcgccgc gatgttcacc gcgtcgccga tgaccgtgta ctgcagggtt cgcgacgacc
     2941 cgatcgcgcc ggcgatgacg gagccggtgt tgacgccgat gccgatgctg accttctcgc
     3001 ggccctggtc gacgcgcagc ctattaaact cctcgagcgc gcgcatcatg tcgagcgcgc
     3061 agcgaacgct gcgcagcggc gcgtccatca ggtcgaccgg ggcgccgaac aggccgatga
     3121 tctcgtcgcc gacgtacttg tcgagcgtgc cgccgtgccg gaacagcacg tcgaccatca
     3181 cctcgaagta ctcgttgagc gtgacgacca tctcctcggg cgtgtggcgc tcgctcatcg
     3241 ccgtgaagcc gcggatgtcg gcgaacagca tcgtgacgtc gcgacgggcg ccgccctgat
     3301 cgagggtcag ctggccggcg acgatctggt cgacgaggtt cggcgagagc aggcgctgga
     3361 actgggcgcg ggcggtcgcc tcgtgctcga tcttctgcgc gagcatgttg ttctcgatcg
     3421 cgaccgcggc ctgcgtggcg atgcccgaga acaggagcag gtccttctcg gtgaaggcgc
     3481 cggtcgccag catcgagtcc atgtgcatcg cgccgagcag cttgtcgttg tagagcagcg
     3541 gcacgcacat cgtcgagcgg atgccctgca tgatgatgct cttggccgcc ttgaagcgct
     3601 cgtcgaccat cgcgtccgag gacagcacgg cgcgcttctt ggtgcgcacc tcgtcgagga
     3661 tgctcttgga gagcttgatc tcctcgtcgc gcttttgtcg gacgtagcgc gggatgaagt
     3721 cgtcggtctc gccgtcgagc agcaggatga cggcacggtc ggcgcggatc agcaggaacg
     3781 cctcgtcgac gatcttctgc agcagggtgt cgaggtcggc gtcgatcgac agcttctgcg
     3841 acagctcgtg agcgacgcgc agcttctcgt agtcggcgcg cagcaccgcg acgtccttga
     3901 tgtccttctc gggcaagaac cgcgagttgg ccgacagcgt gctctggacc tggctctgga
     3961 cctggtccgg cgtcatcgtc accttgcgga tcgggagctt gtcttgcggc cgatcctcgg
     4021 tgaagcggta ggtcgtgttg ccgagcgaga tctgatcgcc ggacttcagc gcgcgctcgc
     4081 tgaccttctc gccattgacg aaggtgccgt tgaggctgcc gacatcacga acgacgtatc
     4141 gaccgtcggg gccgagcgtg atccggctgt gctccttcga cacgatgcgg tcgaggacct
     4201 ggatcgagtt gtctggatgg cgtccaagcg tattaaacga cgagaggacg tgctcgaccg
     4261 gatgatcccc tccgagcagg atgagcttcg ctgccatcga caattcggca tcgtagggca
     4321 gcgaactcgt gggatcaacc ggccccaagc gggccgcgcg ctgcttcgcg cgcggcaact
     4381 tcgtggaatg gcgacgattt cagggatgaa ggccccggac agccgaccgc cacccaccgg
     4441 tttgctcggg gagatcccac aggaccattt tcgctcgcgc accacaagcg ctgaggcgaa
     4501 acccagacct gaagtatgat ggcgccgtcc aaacgcggcc tgaagtgtcc tacctgcccg
     4561 catatgtagc tttggagcga tggtggctcg caaagcgcgt ggccgtcgtg tccccgcaag
     4621 agccggaggg cccgcaacgg ctcgccgcgg ccggtgcagg gcgcgtactg gtgctgggcg
     4681 ccccgctggc gccctcgccg ggcgtcgagg tgtgggccgg tccgttcgcc acgcccctgc
     4741 ggctgcccct gcgcgatggc agcgtcgacg cgctgctgtg catcgaggcc tatgccgcgc
     4801 tcggccccga ggaccgggcg gcgctggtgt ccgaggcccg tcgcgtgctc cgggccacag
     4861 gggtcctcgc aatttacagc gcggatctct ccgagaccga ggcgttggtg cgggccaact
     4921 tctccgaggt cgccgcgctc gcgcagctgc cgtggagcgg ttttcgcctc gcgcccgtcg
     4981 gcgcctcgcc gagctccgaa gttcgcctgg tggaagatct ccggaccggc gagccgccga
     5041 ccggctcgta cctggtgctc gcctcggcca ccgagctcgg gccgctcgtg cagcagtgcg
     5101 tgctcgtggc gaccacgccc gagcccgcga gcgcgacgcc gatcgccgcg accaacgacc
     5161 tcgcggtcga cgagacgccg ccgcagccgc agccagggga ggcgctctcg gaggcgttct
     5221 cacggctggc cgcggcgacg accgcgtcgc cgagcagtca acgggtgccg ccgctcgacc
     5281 cgatcgacga gggcgacgtg ctcgcggggc tgttcgaggc gccggccgaa tcggagtcct
     5341 cgcccgaggc gaccgagccg gagccgccgc cgatcgaccc cgcggagctc gccgagctgc
     5401 gcgaggcggt cgatacgctc cggcgcgagc gcgactcgct gcgcgagcgc gcggccacgc
     5461 tgacgcagga gctccgccac gcgcacgccc aggcgatcga cgccggcgcc gagctgcacg
     5521 agctgcgccg tcgcgtcgac acccagcgcc tcgaggacgg ctcgccgctg ctcgacgacg
     5581 ccgggctgcg caacgacgcc gccgcgctga cgcgggtgct cgtcgacgag gtcgcgccga
     5641 tggtcacgcc cgaggcgacg cgaccgaccg ccgacgtggc gatcgatcgc gaccggttgc
     5701 gcgaggagct gacgcggcgc accgcggatc tgcaggagct cgagacgcgt ctgtggcaga
     5761 gcgaggagga ggtccagcgc gagcgcctcg agaacgtccg cctcgtcacc gatgtcgacc
     5821 gcctgcgcga gcaggtcgat cgcagccgga tcgtcgagca cgagcgcatg caggagctcg
     5881 agcgcctcgg ccacgagctg cgccgcctcg agctggccta cgccgagctg cagggcctgt
     5941 acacgaccgg cgaccaccgg ctgcgcgagc tcgaggcgag catggagcgg ggcgacgacc
     6001 cgccggagct cgtcgagctc gaggcgcagg tgcgcgagct caagggcgag cgcgaccagg
     6061 cgcaggcgct cgagcgggcc gcggtcgagc aggcgaggcg acgcgagcgg gagctcgccg
     6121 aggcgggcag gacgatccgc gagctgcggc gcagcgtcga ggagcacgcg agcgtcgcgg
     6181 ccaacctgcg cggcgagctc gcggtgatgc aggtcgaggt cgagcagttc ggcgccgcgg
     6241 tgccggccct gcaggagcgc ctgcgggacc accagcgcaa gatcctcgag cgcgaggagg
     6301 aggccggcga gctccagcgc cggctcgagg agtcggtcgc cgagcagcag gagctgcgtc
     6361 agcggctgcg gcgttacaag caggacctcg aggtcgcggt caacaccaag gaggcgatcg
     6421 agcaggacct cgaccgcctg cgcggcgagc tcgacgcgaa gcggcgctcg gtggagcagc
     6481 tccagcagct ggtcgcgctc ggggcgggga ccggccgg
//
LOCUS       DASWNJ010000524         8048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_115353, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000524 DASWNJ010000000
VERSION     DASWNJ010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 8048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8048
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115353"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VGB85_08875"
     CDS             complement(<1..674)
                     /locus_tag="VGB85_08875"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0134181.1"
                     /translation="MTIAELDIPGFTIDEVLHTSSAWQLARATRQSDGQPVLLKRPRL
                     ASPLRLAELHHELALATRLADPEILRPLAVERGPAGFALIYEPFAGRPLATGDLSEEP
                     AAPRPSAAVQALAIRLCQVIGRLHRAGVVHKNLHPGAVLLAPDGALRLTDLSLASALA
                     REEASVDPPARVRGELAYLAPEQTGRMNRAIDARSDLYALGVLLYEQLTGAPPFRQHD
                     PLELIHA"
     gene            1213..2463
                     /locus_tag="VGB85_08880"
     CDS             1213..2463
                     /locus_tag="VGB85_08880"
                     /inference="COORDINATES: protein motif:HMM:NF037663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="styrene monooxygenase/indole monooxygenase
                     family protein"
                     /protein_id="HEY0134182.1"
                     /translation="MARIAIVGAGTAGLHLALRLQAFGVPVTLYTDRSPADMRAGRLP
                     NTVAHNGATRERERALGVNHWDSEGLDIHSIGFSLSGPLSFRFRGQLDQPGLFVDYRL
                     YQPRLAEDFVARGGQLEVAAIDAAGVGRLAQQHDVVVVATGRAGLGNLFPRMPEHSPY
                     SRPARLLFAGLFHGISPVSPLGMQFNFTAGHGEVFEARMATRHGPVGSLLVEATPGGG
                     FADVATRKVDEDPRGFEVMLLAALREHAPATFERIDPAAFRLTGPTDWLQGGFAPVVR
                     RGYAPVGEGRFALAVGDAHVLHDPIAGQGANAASAAAWELAEVIREAVEGGERFDEGF
                     FQRAEARTWASEHATTLWNNAMLEPPPPHMGQLLFAASQDPRLADVTINTLVFPQKAL
                     ENFAGPESTMAFIARHGQQASSAA"
     gene            complement(2503..3087)
                     /locus_tag="VGB85_08885"
     CDS             complement(2503..3087)
                     /locus_tag="VGB85_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134183.1"
                     /translation="MPRLAFMVMLLGAACTGPSVEPAPVVFDDAEPAPRAQEAPPTID
                     DPDRPLELGPGELMRVRGGGGACGGSSVLVVHEDGRFTNTSHEGCYMGNPKQGPPPPA
                     VEADRLDWHDLARLHALLADPGLPQMLAIHGDGGGSNHPSRSHFMVRTPAGPVGGEYV
                     GVDLRKVSRFHAFVYTLYARLYPCPPGLCDDEDD"
     gene            3782..4951
                     /locus_tag="VGB85_08890"
     CDS             3782..4951
                     /locus_tag="VGB85_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134184.1"
                     /translation="MRRSTTTVAWAVLACACARGDAERYELDGLVVVEDFDEPICAGT
                     FAYFERRLSALERATGLPRDPLGLIFHWIYEPDEIPSQCGELAGACTKGRDLHGQLWS
                     FSHELVHAHLDRLGTPRVWLTEGMATMLAEDLRGEPDPEVTPSDLLRIDDARGLNYTA
                     AAGFTVYLRDRYGMPQLLDYYAATAGADADSSVATFRDVFGDDFSAAEADYLATGLSV
                     SIGSPDCDVAEVAWSGDRWSHSFALTCDEPTSIGPQQSLDEPERSFLWAGVTMTASAG
                     WFAFDLEASGPAWVTIVRCDRPEILYLGAEEPQINAYLDVGRYLVSADAYADEHTSAT
                     VTARRLAEAPDLATVHGAPPPGFVSHLAQATGTTRGVRTAAPPGAAVNPRGAPAR"
     gene            complement(4956..5756)
                     /locus_tag="VGB85_08895"
     CDS             complement(4956..5756)
                     /locus_tag="VGB85_08895"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEY0134185.1"
                     /translation="MPVGGAEDKEQDAVILRRFLAVSGGSAARVVIIPTASRLPETGP
                     RYERIFRHLGAHSAHSIPFESRADAEREDMLERLEQATCIFLTGGNQLQLSTILGGTP
                     VARAIRRLNARGVTVGGTSAGAAILSEHMIAFGQGGATPIAGLASLAPGFGLTNRVVI
                     DQHFRQRDRLGRLLSVLAFNPFAVGIGLDEDTAAFIDPNDVLHVEGAGAITIVDVSRL
                     EHSSMGHAKTGQAICLTGIQLHILTRHGSYNLHTRHAKPPPAPPDDPD"
     gene            complement(5798..6973)
                     /gene="iadA"
                     /locus_tag="VGB85_08900"
     CDS             complement(5798..6973)
                     /gene="iadA"
                     /locus_tag="VGB85_08900"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEY0134186.1"
                     /translation="MLRILRNAEVYAPDHLGKRDLLIADRRIAAMAESLPKLPKGIEH
                     EDVDLKGLRLMPGLIDAHVHLIGGGGESGPATRVPPVHLTQLSKAGITTVIGVLGTDG
                     TTRTVGDLVARTLGLREEGLSAHCYTGSYQIPVPTLTGSVRGDIVYIDPVIGVGELAL
                     SDHRSSQPTLDELLRVASDAHVAGMISGKAGIVHLHMGDGDRGLDLVRRALEHTEIPA
                     RVLQPTHINRKRKLFDEACALASRGGYLDVTAFPEEEDAGDMMYAAEAIARWLHADLP
                     ADRLTCSSDGLGCMPVFDGHGHICAMEVGRPATLLEAIQKLLADKLPLERFLPVFTKN
                     IAELLRWPHKGRVEVGADADLLVMSQTGQVDAVLARGTWLVRDGQPVVFGRFERSSV"
     gene            complement(7011..7778)
                     /locus_tag="VGB85_08905"
     CDS             complement(7011..7778)
                     /locus_tag="VGB85_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134187.1"
                     /translation="MISSRARLLSLPVGFMLVSLFASAGCNFILNPANSDEVIRCKNT
                     TECEREEFFIAELRKERTDAACGAPGGGGGFTTSKTNQVCSLVDKNSVSCGTEALPAG
                     DFADAVDEAIKNDDVYAPCIGDKKGTLGCAPRNGGTCDAGLTPNEFGTCDDGKGQPLI
                     EANGDLQLQDVKDQHCRSYFCDDSFVCNSKTSKCVRCDDSLGVDRVGQGACGDLAING
                     ARSTVYLPQETLEMECPDSSRFELTKFGPVVTVPEMP"
     gene            complement(7775..>8048)
                     /locus_tag="VGB85_08910"
     CDS             complement(7775..>8048)
                     /locus_tag="VGB85_08910"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0134188.1"
                     /translation="ERVGDRVRALVADNGPGLAVAADEAEHIFEAFFTRKPQGTGLGL
                     SIVQQIVQDHEGGVRVASTGPGGTAFEIVLPACDPPAASVSSPGLS"
BASE COUNT         1188 a         2626 c         3026 g         1208 t
ORIGIN      
        1 gcgtggatca gctcgagcgg gtcgtgctgg cgaaacggcg gcgcgcccgt caattgctcg
       61 tagagcagca cgccgagcgc gtagaggtcg ctgcgcgcgt cgatcgcccg gttcatgcgc
      121 ccggtctgct ccggcgcgag gtaggccagc tcgccgcgca cgcgcgccgg cgggtcgacg
      181 gacgcctcct cgcgcgcgag cgccgaggcg agcgagaggt cggtcaggcg cagcgcgccg
      241 tccggcgcga gcaacacggc gccggggtgc aggttcttgt gcacgacgcc cgcgcgatgc
      301 aggcgcccga tcacctggca caggcggatc gccagggcct ggaccgcggc ggaggggcgc
      361 ggtgcggctg gctcttcgga caggtcgccg gtcgccagcg gccggccggc gaagggctcg
      421 tagatgagcg cgaagccggc ggggccgcgc tcgaccgcga ggggtcgaag gatctccggg
      481 tcggcgaggc gagtcgcgag cgcgagctcg tgatgcagct cggcgaggcg cagcgggctg
      541 gcgaggcgcg gacgcttgag caacaccggc tgtccatcgg actgtcgggt cgcgcgagcg
      601 agctgccacg ccgatgaggt gtgcagcacc tcgtcgatcg tgaagcccgg tatgtcgagc
      661 tcggcgatgg tcacactgcc gggccgaagt gtcgcacgta cacggtttac cggggtaatg
      721 gacccgcggc cggccggcgc agcggtctgc acgtgcgggt gaggagggcc acgtggtcgc
      781 ggctcccgcc cgttcacgcg agcgtgcgtg ggaggaagag gtccaggccg ctgccggacc
      841 gatccgggct gatccgccgc tgccgtcgcg gtgatcggcg atgtcccggg acgaccgcga
      901 aggcgcgctg gcgagcatcc acgggccgcg agggcccgcg aagatattgc gcagtccgtc
      961 ctgtgactcc gcgtcggatc tcccgctggc gacgccgggc ccgagcggtc gatgtcgtcg
     1021 cgtcgcgatc gacgtcgggc gcctgtgcga cacggggagc ccgggaggat cacaccgacg
     1081 ccgacgagag gtagtgagat tcgctgcgga ccggaggcgc ccgcgtgtcg attttgcctg
     1141 gcgctcgcgg ctgcgaccgg ctttatcatc cgcgctctgc caccacgtgt ggtggtcatt
     1201 aggaaggcac tcatggcacg catcgcaatc gtcggcgccg gcacggccgg gctgcacctc
     1261 gcgcttcgat tacaagcatt cggcgtcccc gtgacgctct acaccgaccg gtcgcccgcg
     1321 gacatgcgcg cgggacgtct gcccaatacg gtcgcccaca atggtgccac ccgcgagcgc
     1381 gagcgtgcgc tcggggtcaa tcactgggac tccgagggcc tcgacattca cagcatcggc
     1441 ttctcgctca gcgggccgct gtcgttccgc ttccgcgggc agctcgacca gccgggcctg
     1501 ttcgtcgact atcgcctgta tcagccgcgc ctcgccgagg acttcgtggc gcgcggcggg
     1561 cagctcgagg tcgcggcgat cgacgccgcc ggggtcggcc ggctcgcgca gcagcacgac
     1621 gtcgtcgtcg tcgcgaccgg ccgcgcgggg ctcggcaacc tgttcccgcg gatgcccgag
     1681 cactcgccgt acagccgccc ggcgcggctg ctgttcgccg ggctgttcca tggcatctcg
     1741 ccggtgtcgc cgctcggcat gcagttcaac ttcacggccg gccacggcga ggtcttcgag
     1801 gcgcgcatgg cgacgcgcca cgggcccgtg ggcagcctgc tcgtcgaggc gaccccgggc
     1861 ggcgggttcg cggacgtggc gacgcgcaag gtcgacgagg acccgcgcgg cttcgaggtg
     1921 atgctgctcg cggcgctgcg cgagcacgcc ccggcgacct tcgagcgcat cgaccccgcc
     1981 gcgttccgcc tgactggccc gaccgactgg ctgcaaggcg gcttcgcgcc ggtggtgcgc
     2041 cgcggctacg cgccggtcgg tgagggacgc ttcgcgctgg cggtcggcga cgcccacgtg
     2101 ctgcacgacc cgatcgcagg ccagggcgcg aacgcggcct cggcggcggc gtgggagctg
     2161 gcggaggtga tccgcgaggc ggtcgagggc ggcgagcgtt ttgatgaggg gttcttccag
     2221 cgcgccgagg cccgcacgtg ggcgagcgag cacgcgacga cgctgtggaa caacgcgatg
     2281 ctggaaccgc cgccgccgca catggggcag ctgctgttcg cggccagcca ggacccgcgg
     2341 ctcgccgacg tgacgatcaa tacgctggtg ttcccgcaaa aggcgctcga gaatttcgcc
     2401 gggccggaga gcacgatggc gttcatcgcc cggcacggcc agcaggcgtc gagcgcggcg
     2461 taagggctgg gtgtcccttc ggacaggtcc gaagggacag gattaatcgt cctcgtcgtc
     2521 gcacaggccc ggcggacagg gatacagccg cgcgtagagc gtgtaaacga aggcgtggaa
     2581 gcgtgacact tttcgcaagt cgacgccgac gtactccccg ccgaccgggc cggcgggggt
     2641 gcgcaccatg aagtggctgc ggctcggatg gttcgacccg ccgccgtcgc cgtggatcgc
     2701 cagcatctgc gggagaccgg ggtccgcgag cagggcgtgc aggcgcgcga ggtcgtgcca
     2761 gtcgaggcgg tcggcctcga cggcgggcgg aggcgggccc tgcttcgggt tgcccatgta
     2821 gcagccctcg tgcgaggtgt tggtgaagcg gccgtcctcg tgcaccacga ggaccgagct
     2881 gcctccgcac gcacccccgc cgccgcggac ccgcatcagc tcacccggcc cgagctcgag
     2941 cggccgatcg gggtcgtcga tcgtcggcgg ggcctcctgc gcccgcgggg ccggctcggc
     3001 gtcgtcgaac accaccgggg ccggctcgac cgatgggccc gtgcacgcgg cgccgaggag
     3061 catgaccatg aaagcgagac gaggcatcgc atcacgatat ccgaactcgc ggagaagtgg
     3121 cgagcgcccg gacgttcgtc gccgcgcggc ctgcgggctg atctgatccg ggtcatcggc
     3181 gcgctggcga aagtgctgtc gatgacgacg cggtcggtta gtccgaagcc ggacgcgagg
     3241 cggcaagaag ggggttaccg ccgacgcgac cgttgtcgtg ctgtgtcgct cagctcagcg
     3301 cgttgcgggc gtggagttgc ggtttccggc atagcctcct agtctttgcg tggaggtcag
     3361 catgagccga cgaacgcgag cctggcagct tcttataggt gcgagcctgt tgctagggtg
     3421 caacggatca gggtgcaata acggatccga gaccacgagc ggaaagcttg tgggcgtcga
     3481 gtgtggagcc cacaccaagt gtccgtttgg ctacgagtgc gactacggcg cgaaggaccc
     3541 caaccacccc aagagcgtcg gcctttgcca gtacgtggag tgcaagctga cagacctctg
     3601 cgagaagccg cacaaagagt gcccgctacc tcaagagacc gcgatatgtg acaagtttga
     3661 caacgacaag tactgcgact gtgtgaggcc cagctcgcag gatgtgccag gcacccccac
     3721 gacggggggc cctccggccc ctacaaccgg ggccaccccc actagcgggg acaagccgta
     3781 ggtgcggcgc tcgacgacga cggtcgcatg ggctgtgctt gcttgcgcgt gcgcacgcgg
     3841 cgacgcggag cggtacgaac tggacggcct ggtcgtggtc gaggatttcg acgagccgat
     3901 ctgtgccggg acattcgcct acttcgagcg acgcctgagc gcgctcgagc gcgcgaccgg
     3961 actaccccgc gacccgctcg ggctgatctt ccactggatc tacgaacccg acgagattcc
     4021 atcgcagtgc ggtgagcttg ccggcgcatg caccaagggg cgggacctcc atgggcagct
     4081 gtggtcgttc agccatgagc ttgtgcatgc gcacctggac cgactcggca ccccgcgcgt
     4141 gtggctcacc gaaggcatgg cgacgatgct cgcggaggac ctccgcggcg agcctgatcc
     4201 agaggtcacc ccctcggact tgctacggat cgacgacgcg cgagggctga actacaccgc
     4261 cgccgccggg ttcacggtct acctgcgcga caggtacggc atgccgcagc tcctcgacta
     4321 ctacgcggcc acggccgggg ccgacgcgga cagctccgtg gcgaccttcc gggatgtctt
     4381 tggcgatgac ttctcggccg cggaggcgga ttaccttgcg accggcctgt cggtgtcgat
     4441 cggctccccc gactgcgatg tcgctgaggt cgcatggagc ggcgataggt ggtcgcacag
     4501 cttcgccttg acctgtgacg agccgacatc gatcggcccg cagcagtcgc tcgacgagcc
     4561 ggagcgttcg tttctctggg caggcgtcac gatgacggcc tccgccggct ggttcgcgtt
     4621 cgatctcgag gcgtcagggc cggcgtgggt caccatcgtc cgctgtgacc gccccgagat
     4681 cctgtatctg ggggccgagg aaccgcagat caacgcctat ctcgacgtcg gacgctacct
     4741 tgtctcggcc gacgcctacg ccgacgagca cacgagcgcc acggtcacgg ctcgccggct
     4801 cgccgaggcc ccagacctgg caaccgtgca cggcgccccg ccgccgggct tcgttagcca
     4861 tctggcgcag gccacaggca caactagagg ggtgcgaacg gcggcgcccc ccggcgccgc
     4921 cgtcaacccc cgtggcgctc cggcacgctg agccttcaat ccgggtcgtc gggcggggcc
     4981 gggggcggct tggcgtggcg cgtatgaaga ttgtacgagc cgtggcgggt caggatgtgc
     5041 agctggatgc cggtgaggca gatcgcctgg ccggtcttgg cgtggcccat cgaggagtgc
     5101 tcgaggcggg atacgtcgac gatggtgatc gcgccggcgc cctcgacgtg gaggacgtcg
     5161 ttggggtcga tgaaggccgc cgtgtcctcg tcgaggccga tgccgacggc gaaggggttg
     5221 aaggcgagca ccgagagcag gcggccgagg cggtcacgct ggcggaagtg ctggtcgatg
     5281 acgacgcggt tggtcaggcc gaagccgggc gcgagcgagg cgaggccggc gatcggtgtc
     5341 gcaccaccct gaccgaaggc gatcatgtgc tccgacagga tcgcagcgcc ggccgaggtg
     5401 ccgccgacgg tcacgccgcg cgcgttgagc cggcggatcg cgcgggcgac gggagtaccc
     5461 cccaggatgg tggagagctg gagctggttg ccgccggtca ggaagatgca ggtcgcctgc
     5521 tcgaggcgct cgagcatgtc ctcgcgctcg gcgtcggcgc ggctctcgaa cgggatgctg
     5581 tgggccgagt gcgcgccgag gtggcggaag atgcgttcgt aacgcgggcc ggtctcgggc
     5641 aggcgcgagg cggtcgggat gatgacgacg cgcgcagcgg agccgcccga gacggcgagg
     5701 aagcggcgca ggatgacggc gtcctgttcc ttgtcctcgg cgccgccgac cggcacgatc
     5761 catccgcgag ggcgggactc atcgactttc gctgggctca aacgcttgac ctctcgaagc
     5821 gcccgaatac caccggttgc ccgtctctca ccagccacgt accgcgcgcc aatactgcgt
     5881 ccacctggcc ggtttgtgac attacgagca gatcggcgtc ggccccgacc tcgacccggc
     5941 ccttgtgcgg ccagcgcagc agctcggcga tattttttgt gaagaccggg agaaagcgct
     6001 cgagcgggag cttgtcggcg agcagcttct ggatcgcctc gagcagcgtc gccgggcggc
     6061 cgacctccat cgcgcagatg tggccgtggc cgtcgaacac gggcatgcag ccgaggccgt
     6121 ccgaggagca ggtcaggcgg tcggccggca ggtcggcgtg cagccagcgg gcgatcgcct
     6181 cggcggcgta catcatgtcg ccggcgtcct cctcctcggg gaaggcggtg acatcgaggt
     6241 agccgccgcg gctggcgagg gcgcaggcct cgtcgaacag cttgcgcttg cggttgatgt
     6301 gcgtgggttg cagcacgcgc gcggggatct cggtgtgctc gagggcgcgg cggacgaggt
     6361 cgaggccgcg gtcaccgtcc cccatgtgca gatgcacgat gccggccttg ccggagatca
     6421 tgccggcgac gtgggcgtcc gaggcgacgc gcagcagctc gtcgagggtc ggctgcgagg
     6481 agcggtggtc cgagagcgcg agctcgccga cgccgatcac ggggtcgatg tagacgatgt
     6541 cgccgcggac cgagccggtc agcgtcggga cgggaatttg atagctgccg gtgtagcagt
     6601 gggcgctgag gccctcctcg cgcaggccga gggtgcgcgc gacgaggtcg ccgacggtgc
     6661 gcgtggtgcc gtccgtgccg agcacgccga tcacggtggt gatgccggcc ttgctcagct
     6721 gcgtgagatg gaccgggggc acgcgggtcg ccggtccgga ctcgccgccg ccgccgatca
     6781 ggtgcacgtg ggcgtcgatc aggccgggca tcaggcgcag acccttgagg tcgacgtcct
     6841 cgtgctcgat gcccttgggc agcttcggga gggactcggc catcgccgcg atgcgacggt
     6901 cggcgatcag gaggtcacgc ttgccgaggt ggtcgggggc gtagacctcg gcgttacgga
     6961 gaattcgcag catgatgcag ctaatggagc cgttcctggc ccggggccac tcagggcatc
     7021 tcgggcacgg tcacgaccgg cccgaacttg gtcagctcga acctcgagga gtccgggcac
     7081 tccatctcga gggtctcctg cgggaggtag acggtcgagc gggccccgtt gatcgccagg
     7141 tcgccgcagg cgccctgacc cacgcggtcg acgccgaggc tgtcgtcgca gcggacgcac
     7201 ttgctggtct tgctgttaca gacgaaggag tcgtcgcaaa agtacgagcg gcagtgctgg
     7261 tccttgacgt cctgcagctg gaggtcgccg ttggcctcga tcagcggctg gcccttgccg
     7321 tcgtcgcagg tgccgaactc gttgggggtc aggccggcgt cgcaggtgcc gccgttgcgc
     7381 ggggcgcagc cgagggtgcc cttcttgtcg ccgatgcagg gggcgtagac atcgtcgttc
     7441 ttgatcgcct cgtcgacggc gtcggcgaag tcgccggcgg gcagggcctc ggtgccgcag
     7501 gagacgctgt tcttgtcgac gagcgagcag acctgattcg tcttcgaggt ggtgaagccg
     7561 ccgccgccac cgggagcgcc gcaggcggcg tcggtccgct ccttgcgcag ctcggcgatg
     7621 aagaactcct cccgctcaca ctcggtggtg ttcttgcagc ggatgacctc atccgagttg
     7681 gccgggttga ggatgaagtt gcagccggcg gaggcgaaca gcgacaccag catgaacccg
     7741 acggggaggg agaggagtct ggcgcgggac gagatcatga gaggcctggc gaggatacgg
     7801 aggcggctgg gggatcgcaa gccggaagga cgatctcgaa cgcggtgcct ccggggccgg
     7861 tcgaggccac gcggaccccg ccctcgtggt cctggacgat ctgctggacg atcgagaggc
     7921 cgaggccggt gccctgcggc ttgcgggtga agaaggcctc gaagatgtgc tcggcctcgt
     7981 cggcggcgac ggccaggccg gggccgttgt cggcgacgag ggcgcgcacg cggtcgccga
     8041 cccgctcg
//
LOCUS       DASWNJ010000525         2363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_645082, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000525 DASWNJ010000000
VERSION     DASWNJ010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645082"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1558)
                     /locus_tag="VGB85_08915"
     CDS             complement(143..1558)
                     /locus_tag="VGB85_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134189.1"
                     /translation="MPGPVVSSRLFFIAFATLAACNQAAPQDDLHEAPPPEDLPPRPE
                     PPPEGETFEEMKARFEGEKPDVMAKQLALLKERYDLRDDPSGVKMTRGKPVQKGVRVK
                     LPDGMTWDELGALSPSEIKAMDVFPAGFLPLPHPNQPEGGMLFPKPHIDALLEQTGRD
                     LERFDLDFDLPEHVMPEFPPAIFLTTRPDLGDVSKGELITTHNFYRIFKGVLNDKDLD
                     GLRLLVFPFAQQQFNLIDDRRSAEPSLGVSCFDCHANGHTNGATHLVGDIRPQEFRNR
                     IETPSLRGVNIQRLFGSQRALMTIEDFTEFEQRAAYFDGDIDKAALKGINPLDRASQV
                     HHMAEMERLLDFPPAPKLDVLGKLNDSASPSELAGEALFFGKALCSECHTPPYFTDNT
                     MHDLELERFFSQKVINGLVADGDGPIKTFPLRGIKESPPYFHDGRLLTLEDTVEFFNI
                     VLELHLTPTEKTDLLAYLLVL"
     gene            1921..>2363
                     /locus_tag="VGB85_08920"
     CDS             1921..>2363
                     /locus_tag="VGB85_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0134190.1"
                     /translation="MGFSAHSARGTSELRSGDVFANRFEIDRVAGSGGMGTVYRARDR
                     FTGDMVALKLLHNQSADPHDAERFAREAQFLSELRHPAIVSHVAHGFTPDGQRYLALE
                     WLDGEDLAQRIARGPLTVREGLLLLQHVAGALAAAHEHGVVHRDI"
BASE COUNT          408 a          737 c          794 g          424 t
ORIGIN      
        1 gctcgtgcag ttgtccgggc tcaagaggac agattcgact ccggggacct tgctcagcct
       61 cgggctcgag acccacgttc agatcggtct cccggagtct ttggacagga actctcgctc
      121 aacgcccgcc gaacatacaa gatcagagga ccaacaaata cgcgagcagg tccgtcttct
      181 cggtcggggt caggtgcagc tcgagcacga tattaaaaaa ctcgaccgtg tcctcgagcg
      241 tcagcaggcg gccatcatga aaatacggcg gcgactcctt gatgccgcgc agcgggaagg
      301 tcttgatcgg tccgtcgccg tcggcgacca ggccattgat caccttttgg ctgaagaagc
      361 gctcgagctc gaggtcgtgc atcgtattgt cggtgaagta cggcggcgtg tggcactcgg
      421 agcacagcgc cttgccgaag aacagcgcct cgccggcgag ctcggacggg ctcgcggagt
      481 cgttgagctt gccgagcacg tcgagcttcg gcgcgggcgg gaagtcgagc aggcgctcca
      541 tctcggccat gtggtgcacc tggctggcgc ggtcgagcgg gttgatgccc ttgagcgccg
      601 ccttgtcgat gtcgccgtcg aagtaggcgg cgcgctgctc gaactcggtg aagtcttcga
      661 tcgtcatcag cgcgcgctgc gagccgaaca gccgctggat attgaccccg cgcagcgagg
      721 gggtctcgat gcggttacga aactcctggg gacgaatgtc gccgacgagg tgggtcgccc
      781 cgttggtatg gccattggcg tgacaatcga agcacgacac gccgaggctc ggctccgccg
      841 accgccggtc atcaatcaaa ttaaattgct gctgcgcgaa cggaaacacc aaaagccgca
      901 gcccgtcgag gtccttgtcg ttgaggaccc ccttgaagat cctgtaaaaa ttgtgggtcg
      961 tgatcagctc gcccttcgag acgtcgccga ggtccgggcg cgtcgtcagg aagatcgccg
     1021 gcgggaactc cggcatcacg tgctcgggca ggtcgaagtc gaggtcgaag cgctcgaggt
     1081 cgcgaccggt ctgctcgagc agcgcgtcga tgtgcggctt gggaaacagc atgccgcctt
     1141 cgggctggtt ggggtgcggc aggggcagga agccggccgg gaacacgtcc atcgccttga
     1201 tctcgctcgg cgacagcgcg ccgagttcgt cccaggtcat gccgtcgggc agcttgacgc
     1261 gcacgccctt ctgcaccggc ttgccgcgcg tcatcttgac ccccgagggg tcgtcgcgca
     1321 ggtcgtagcg ctcttttaat aaggcgagct gcttcgccat cacgtccggc ttctcgccct
     1381 cgaagcgggc cttcatctct tcaaatgtct cgccctccgg cggcggctcc ggacgcggcg
     1441 gcaggtcctc gggtggcggg gcctcgtgca ggtcgtcctg cggggccgct tgattacacg
     1501 cggcgagcgt ggcgaatgcg atgaagaaca ggcgggagga gacgacagga cctggcatct
     1561 tcggcttata tcgaagcgaa cgagaaacca ccagcccgtg cgctgcatat gtgatttgcg
     1621 tgcttcatcg gtaatgccat caaacgcgca tgtggcgaca tatccacccg gtctcgccgg
     1681 tgaagtccct gtcgctatcg tggtaaaagc cgctttggac ggataaattc ctggcctgaa
     1741 ggccatggat acacggcagt acgagcgccg cggcgctggc ggcgcagtgc catgatagct
     1801 gtgtgcgcca gatcgctggc ggatcggggt ttggcattat cgggccaccc agggggccga
     1861 gcgggcggcc cggcagtgag ggtttgaaac cccaccccga gggcgtacga tcgggaaatc
     1921 atggggtttt ctgcccattc ggcgaggggg acctcggagc tgcgctccgg cgacgtcttc
     1981 gccaaccggt tcgagatcga ccgggtggcc gggtcgggcg gcatgggcac ggtctatcgc
     2041 gcgcgcgacc gcttcaccgg cgatatggtg gcgctcaagt tactgcacaa tcaaagcgcc
     2101 gatccgcacg acgccgagcg attcgcccgc gaggcgcaat tcctgtccga gctgcggcac
     2161 cccgcgatcg tgtctcacgt cgcgcacggc ttcacgccag acggccagcg atatctcgcc
     2221 ctcgaatggc tcgacggcga ggacctggcc cagcggatcg cccgcggtcc gttgacggtc
     2281 cgcgagggcc tgctgctcct gcagcacgtc gccggggccc tcgcggccgc ccacgagcac
     2341 ggcgtcgttc atcgtgacat caa
//
LOCUS       DASWNJ010000526         3693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_539943, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000526 DASWNJ010000000
VERSION     DASWNJ010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539943"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="VGB85_08925"
     CDS             <1..981
                     /locus_tag="VGB85_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134191.1"
                     /translation="GELSHKECVALCTAPIVTSDPTTGGGSCTGPGEPITGMSTGSGT
                     EAGSSTETGSGTETGSGTDAGTGSTTGGCMDVTPVDSEGGGEDVVSCQALKEAVDYVR
                     CTVADCVFGRRPEGLRSGGRALAGDAGGLWLAEAAHLEAASVPAFERLAAALAMHGAP
                     EELCRRALAAAQDERRHAAQMTTLAAEHGAAAAALELDEAVMPTLIDLARDNTVQGCV
                     GETWAALLARHQADAAGLPQVRGVMAAIAADETEHAELAWAIDEWLCTRLTAEERAEV
                     VAARIAACEVLRERVTDGVDHPALQELGVPRRATAVALYDGLSRALWLAA"
     gene            complement(985..3615)
                     /gene="thrA"
                     /locus_tag="VGB85_08930"
     CDS             complement(985..3615)
                     /gene="thrA"
                     /locus_tag="VGB85_08930"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF006959.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HEY0134192.1"
                     /translation="MKPRDPTDTSPRDTSPGASELLLDASEPSPDASEPLLDASEPLD
                     ASEPLLHASPLLDAREFLLDASEPSPDPSSASSEPSSGGAPSPHTSPDSPAVATLAPD
                     ASPADAPVLDEDIEHLEDAEHLEDDTDEPTPPTDLVTHVTDLLAPLRQVLTGVALLRQ
                     CTSQTLDLVLSFGERLSASILAALLPIRGLPARFVDARDWLVTDDRFGAALVDDAATR
                     TRLADLAPLLGETIAVHTGFLGATIDGRSTTLGRNGSDYTATLLAAMLTAAEVQIWTD
                     VPGVMTGDPTILRDAYPIASMSYAEALELANFGARMFHPRTMVPLIEHAIPLRIRSTM
                     DPDAEGTRIDLGGSSGSGPTCVTSLENLALLDVRWRRLTHEAGVGMRVLRALETAGVT
                     VWMANQAAHGQAVAVVIPEAAVDRARAAIDLELALELQRGEVDSPGVRAPITLLTLVA
                     ERMDPKTAPSQFFRALGQVGVPVRAIAQGMSSRSISCVIDGGETALAVRTVHAAFNFA
                     HEQMSLFVMGHGTVGAELLAQVGRQRAQLKADHGIALTVVGLADSKRALLREHGVDLG
                     SWREALAAAEPFGPEARAQALIPLLERLRRLPAPVLVDCTAADDMDRVYAEAFARGIH
                     VVTANKKPLTLATGPNGQLREVARCAHAAYRYETTVGASLPVIATLADLVRTGDRVHR
                     VEGAFSGSLGFITGEVMRGVPLSHAVRMARERGFTEPHPRDDLSGLDVARKALILARE
                     LGLKIELDDIAVHPLIHHDLLRADDLEVFFNGLADYDATFASSLADVRAQNRVLRYLA
                     RIDLSPDTGEFRVRVGPIDVALDHPAATLRGAEAFVAFTTERYEEYPLVVRGAGAGGA
                     VTAAGVLADILKVAQTLRGR"
BASE COUNT          486 a         1148 c         1575 g          484 t
ORIGIN      
        1 ggcgagctgt cgcacaagga gtgtgtcgcg ctgtgtaccg ccccgatcgt gacgtcggac
       61 ccgaccacgg gtggggggag ctgtacgggc cctggggaac cgatcacggg gatgagcacg
      121 gggagcggca cggaggcggg cagcagcacg gagacgggca gcggcacgga gacggggagc
      181 ggcacggatg ccggcacggg cagcaccacc gggggctgta tggacgtgac ccccgtcgat
      241 tccgagggcg ggggagagga tgtggtgtcg tgccaggcgc tcaaggaggc ggtcgactat
      301 gtgcgctgca cggtcgcgga ttgtgtattc ggccggcgac cggaagggct gcgctccggc
      361 gggcgggcgc tcgccggtga cgcgggcgga ctgtggctcg ccgaggcggc gcacctcgag
      421 gccgcgtcgg tgccggcctt cgagcgcctc gcggcagcgc tcgcaatgca tggagcgccg
      481 gaggagctgt gcaggcgggc gctggcggcc gcgcaggacg agcgccggca cgcggcgcag
      541 atgacgacgc tggcggccga gcacggggca gcggcggcgg cgctcgagct cgacgaggcg
      601 gtgatgccga cgttgatcga tctggcgcgc gacaacacgg tgcagggctg cgtcggggag
      661 acctgggccg cgctgctcgc tcgccaccag gccgacgcgg ccgggctgcc gcaggtccgc
      721 ggagtcatgg ccgcgatcgc ggccgacgag acggagcacg ccgagctcgc gtgggcgatc
      781 gacgagtggt tgtgtacgcg gctgacggcg gaggagcgcg ccgaggtggt cgcggcgcgc
      841 atcgccgcgt gcgaggtgtt gcgcgagcgg gtcacggatg gcgtcgatca tccggcgttg
      901 caggagctcg gcgtgccgcg ccgggccacc gcggtcgcgc tgtacgacgg gctgtcacgc
      961 gcgctgtggc tcgcggcgtg aagctcagcg gccgcgcagg gtctgggcca cctttaaaat
     1021 atcggcgagc acgccggcgg ccgtgacggc gccgcccgct ccggcgccgc gcacgaccag
     1081 cggatactct tcgtagcgtt cggtggtgaa ggcgacgaag gcctcggcgc cgcgcagggt
     1141 ggcggcgggg tggtcgaggg cgacgtcgat cgggccgacg cggacgcgga actcgccggt
     1201 gtcgggcgag aggtcgatgc gggccaggta gcgcagcacg cggttctggg cgcgcacgtc
     1261 ggcgaggctg ctggcaaagg tcgcgtcgta gtcggcgagg ccattgaaaa aaacctcgag
     1321 gtcgtcggcg cgcagcaggt cgtggtggat caacgggtgc acggcgatgt cgtcgagttc
     1381 gatcttcagg ccgagctcgc gcgcgaggat cagcgccttg cgggcgacgt cgaggccgct
     1441 gaggtcgtcg cgcgggtggg gctcggtgaa gccgcgctcg cgggccatcc ggaccgcgtg
     1501 cgacagcggg acgccgcgca tgacctcgcc ggtgatgaag ccgagcgacc cggagaaggc
     1561 gccctcgacc cggtgcacgc ggtcgccggt gcgcacgagg tcggcgaggg tggcgatgac
     1621 gggcagggag gcgccgacgg tggtctcgta gcggtaggcc gcgtgggcgc agcgggcgac
     1681 ctcgcgcagc tgtccattgg gacctgtcgc aagggtcagg ggcttcttgt tggcggtgac
     1741 gacgtggatc ccgcgcgcga aggcctcggc gtagacgcgg tccatgtcgt cggcggcggt
     1801 gcagtcgacg agcacggggg cgggcaggcg acgcaggcgc tcgagcaggg ggatcagggc
     1861 ctgcgcgcgc gcctcggggc cgaagggctc ggcggcggcg agcgcctcgc gccagctgcc
     1921 gaggtcgacg ccgtgctcgc gcagcagggc gcgcttgctg tcggcgaggc cgacgacggt
     1981 cagcgcgatc ccgtggtcgg ccttgagctg ggcccgctgt cgcccgacct gcgcgagcag
     2041 ctcggcgccg acggtcccgt ggcccatcac gaacaggctc atttgttcgt gggcgaagtt
     2101 gaaggcggcg tgcacggtgc gcacggcgag ggccgtctcg ccgccgtcga tgacgcagga
     2161 gatcgatctc gagctcatgc cctgggcgat cgcgcggacc ggtactccga cctggccgag
     2221 ggcgcggaag aactgcgagg gcgcggtctt cgggtccatg cgctcggcga cgagggtcag
     2281 cagggtgatc ggcgcgcgca cgccggggct gtcgacctcg ccgcgctgca gctcgagcgc
     2341 gagctcgagg tcgatcgccg cgcgagccct gtcgacggcg gcctcgggga tcacgaccgc
     2401 gacggcctga ccgtgcgcgg cctggttggc catccacacg gtgacgcccg cggtctcgag
     2461 ggcccgtagc acgcgcatgc cgacgcccgc ctcgtgggtg aggcggcgcc agcggacgtc
     2521 gagcagcgcg aggttctcga gcgaggtgac acaggtcggg ccggacccgc tcgagccgcc
     2581 gaggtcgatg cgcgtgccct cggcgtcggg gtccatcgtg ctgcggatcc gcagcgggat
     2641 cgcgtgctcg atcagcggga ccatcgtccg cgggtggaac atgcgcgcgc cgaagttggc
     2701 gagctcgagc gcctcggcgt acgacatgct ggcgatcggg taggcgtcgc gcaggatcgt
     2761 cgggtcgccg gtcatcacgc ccggtacgtc ggtccagatc tggacctcgg ccgcggtgag
     2821 catggcggcg agcagggtgg cggtgtagtc ggagccgttg cggcccagcg tggtgctgcg
     2881 accgtcgatc gtcgcgccga ggaagccggt gtgcacggcg atcgtctcgc cgaggagcgg
     2941 cgcgaggtcg gcgaggcggg tccgcgtcgc ggcgtcgtcg acgagcgcgg cgccgaagcg
     3001 gtcgtcagtg acgagccagt cgcgggcgtc gacgaagcgc gccggcaggc cgcggatcgg
     3061 gagcagcgcg gcgaggatgc tggcgctcag gcgctcgccg aaggagagca cgaggtcgag
     3121 cgtctgcgag gtgcactggc gcagcagcgc gacgccggtc aacacctgac gcaggggcgc
     3181 gagcaggtcg gtgacgtggg tgacgaggtc ggtcgggggc gtcggctcgt cggtgtcgtc
     3241 ctcgaggtgc tcggcgtcct cgaggtgctc gatgtcctcg tcgaggaccg gcgcgtcggc
     3301 gggggaggcg tcgggcgcga gggtcgcgac ggcggggctg tcgggggacg tatggggcga
     3361 cggcgcgccc ccggacgagg gctcgctcga cgcggacgag ggatcgggcg aaggctcgct
     3421 cgcatcgagc aagaactcgc gcgcatcgag cagggggctc gcgtggagca ggggctcgct
     3481 cgcatcgagg ggctcgctcg catcgagcag gggctcgctc gcatcgggcg agggctcgct
     3541 cgcatcgagc aggagctcgc tcgcaccggg tgaggtgtcg cgcgggctcg tgtcggtcgg
     3601 gtcgcgcggc ttcacgtgcg gtggcggggg cgggggggcg cggcgtggtg caggtgcctc
     3661 gacgggcgtt tcggcgggcg gatgggcggc gcg
//
LOCUS       DASWNJ010000527         3063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_679558, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000527 DASWNJ010000000
VERSION     DASWNJ010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679558"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            584..2833
                     /locus_tag="VGB85_08935"
     CDS             584..2833
                     /locus_tag="VGB85_08935"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEY0134193.1"
                     /translation="MQAIQALHLLLLQLFPENTELKNLLIGNYPELAADLPGDVTTRS
                     NYSHAIIALLQARNYLDAQFFQILVAAFPRRVDDIVSVAARYIDPKVAEQLRAPASKA
                     VPAGAAPGRSSEQAPPREDIASAPTFVVLTALQLETRAVLAQLDDLGELELPSGTILD
                     VGHLRGAATGVRIGVIECGPGNSAAALLCAEAIHAFKPRALVFVGVAGGLKDVACGDV
                     VVATKVYGYESGKSETTFKPRPDVGASAHRLVQRARAIARRDAWFNRIPRSTTPRPNS
                     LVAPMAAGEQVVADTESPTYKFLREAYSDAVAVEMEGRGFLAAAFVHHVEAIVIRGIS
                     DCIARKEESDKAGMQSIAAANAAAFAAEMLAQLAVRPADGPPDVEVPPQRPPRPAAAR
                     VILAIGAQPADMAPLSLGRDVQEIRNYLARLGATDRFRLELVVDMQAHALFQEISAFR
                     PSILHIHVHGREDDGALYFLGPGNTAQPVLPDDLVELMRRFAGTIDLVVLQACYSERL
                     AEKLSAAGIPAVGVPGTLSSSNAALFASNLYGNLANGLNIADACAVATMVLRVAGTRA
                     QSMARLYEAAHSREPMFPTRTPTETLSGAAADAIADPSIALLCNARDQAWVDKAREAF
                     SYLPDGSVSIWDPRLIRPGMNIDTAIEKALNAAAAVCVFLSTSFTSDPRLMQHLSGLV
                     RRAKDGGLLLIWIYARPCNPPPMLAPLQPANEPHEALSGIPEADAEKILTRIVAAVVA
                     MLKLLPTPR"
     gene            complement(2820..>3063)
                     /locus_tag="VGB85_08940"
     CDS             complement(2820..>3063)
                     /locus_tag="VGB85_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134194.1"
                     /translation="LPTDARPQVWEWRDKSTPRNHVRDKKAPEKELISIPFTGPALFI
                     HHWMVVPPGAVSEDIKDAAPSSGTVKFKTRPRFNGE"
BASE COUNT          510 a         1065 c          964 g          524 t
ORIGIN      
        1 agcgacccgc ctgagagctc gtacgtcgcc acgcccgcgc cggtgtacac agccatgccg
       61 tggtcgctcg acattcttga cagtgtcgcc gagcgcgtga gagctgtcca ataacgccgt
      121 caagccgcga ggatggcgca gaggcccccg cacacgcctg ccggggcgga catggacccg
      181 gcaggcgagc gaccgcgtcg tgggtacgtg cgcgagccac ggggcgatca cccggttcct
      241 gtccaccggg atgccggact tgatcctcct caccggcgcc agtcgacgca agagcgcacc
      301 ctaatacgag ctgttcaact ctggttgctc aatcggcgac gcgatcgtcg ccaccgattg
      361 cgttctccgc ggtcgagcgc gatggtctgt ggcatccgct ggggcactgc ggcgctcatc
      421 ctctgccgcg agtccacgag gccggccgtc gtatctcctc tcgaaaccct tcgacccgag
      481 atcacgggat catacgaccg gacacgcagg gatggttact gctccaagcg cctgaacgtc
      541 tggccaggca tgggcgaggc gcggttatgg tagcgtctgt ccggtgcaag ctatccaggc
      601 gcttcacctt ctattgctcc agctattccc cgaaaacacg gagctgaaga atctcctgat
      661 cgggaattat ccagagctgg cggctgatct gcctggagac gtaacgacgc gcagcaatta
      721 cagccatgcg atcatcgcgt tattgcaggc ccggaattac ctcgacgcgc aattctttca
      781 gatcctcgtc gccgccttcc ctcgccgtgt cgacgacatc gtaagtgtcg cagcccggta
      841 tatcgaccca aaagtggccg agcagcttcg tgccccggct tcgaaggcgg tgccggcggg
      901 cgccgctccg ggccgctcat cggagcaggc gcctccacgt gaggacatcg cttcggcgcc
      961 gacgttcgtc gtgctcaccg ctctgcagct cgagacgagg gccgtcctcg cgcagctcga
     1021 cgacctcggc gaactcgagc tgccgagcgg caccatcctc gacgtcggtc acctccgcgg
     1081 cgccgccacg ggtgtccgga tcggcgtgat cgagtgcgga cccggcaaca gcgcggcggc
     1141 gctgctgtgc gccgaggcga tccacgcgtt caaaccgcgg gcgctggtgt tcgtcggagt
     1201 cgctggtggc ctcaaggatg tcgcgtgcgg ggatgtcgtg gtcgccacga aggtctacgg
     1261 ctacgagtcc ggcaagagcg agacaacgtt caagccgcga cccgacgtcg gcgcatcggc
     1321 gcatcgcctc gtccagcgcg ccagggcgat cgccaggcgc gacgcgtggt tcaatcgtat
     1381 tccgcgctcg acgacccccc gaccgaactc cctggtcgct ccgatggccg ccggcgagca
     1441 ggtcgtcgcc gacactgaat ctcccacgta caaattcctg cgcgaagcct acagcgacgc
     1501 tgtcgccgtc gagatggagg gccgcggctt cctcgcggcg gcgttcgtcc atcacgtgga
     1561 ggcgatcgtc atacgcggca tttcagactg catcgctcgc aaggaggagt ccgacaaggc
     1621 cggcatgcag tcgatcgccg ccgcgaacgc ggccgccttc gccgcggaaa tgctcgcgca
     1681 gctggccgtt cggccggccg acggcccacc cgacgtggaa gtcccgcccc aacgaccgcc
     1741 ccgacccgca gcggcacgcg ttatcctagc catcggtgcg cagccggccg acatggcacc
     1801 gctctcgctg gggcgggatg tccaggagat ccgcaattac ctcgccaggc tcggggccac
     1861 cgatcgtttc cggctcgagc tcgtcgtcga catgcaggcc cacgcgctat tccaggagat
     1921 cagcgctttc aggccttcga tcctgcatat ccacgtgcac ggccgtgagg acgatggagc
     1981 gctctatttc ctggggcccg gaaacaccgc gcagccggtg ctcccggacg atctcgtcga
     2041 actgatgcga cgattcgccg ggacgatcga tctcgtagtc ttgcaggcct gctacagcga
     2101 gcgcctggcc gagaagctgt ccgcggccgg gatccccgcc gtgggtgtcc ccgggaccct
     2161 ctcgtccagc aacgcggcgc tgtttgccag caatctctac ggcaatctgg ccaacggcct
     2221 aaacatcgcc gacgcatgcg ccgtcgccac gatggtgctc cgggtcgccg ggactagagc
     2281 tcaatccatg gcccgcctct acgaagcggc ccattcgaga gagccgatgt tcccgacccg
     2341 gaccccgacg gaaacacttt ccggtgctgc cgccgacgcc atcgcagatc cgtccatcgc
     2401 cctgctgtgc aacgcgaggg atcaggcgtg ggtcgacaag gcacgcgagg cgttctccta
     2461 tctcccggac ggcagcgttt cgatctggga tcccagactg atccgacccg ggatgaatat
     2521 cgacacggcc attgaaaagg ctctgaacgc cgccgcagcg gtgtgcgtgt tcctgagtac
     2581 gtcgttcacc tcggatcccc gcctgatgca gcatctgtcg gggctggtca ggcgcgcaaa
     2641 ggacgggggg ctgctcctca tctggatcta tgcgcgccct tgcaatccgc ctcccatgct
     2701 cgccccgctg cagcccgcga acgaaccgca tgaggctctg agcggcatcc cggaggccga
     2761 cgccgagaag atcctgacac ggatcgtcgc cgccgtcgtg gcgatgttga agctgctgcc
     2821 tactccccgt tgaaccgcgg ccgcgtcttg aacttcacgg tgcccgacga cggtgccgcg
     2881 tccttgatgt cctccgagac cgctccgggc ggcacgacca tccagtgatg gatgaacagc
     2941 gcgggccctg tgaacgggat cgagatcagc tccttctcgg gcgccttctt gtcgcgaaca
     3001 tgattgcggg gcgtggactt gtcgcgccac tcccagacct gcggccgcgc gtccgtaggg
     3061 agc
//
LOCUS       DASWNJ010000528         1670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_150744, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000528 DASWNJ010000000
VERSION     DASWNJ010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150744"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VGB85_08945"
     CDS             <1..372
                     /locus_tag="VGB85_08945"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEY0134195.1"
                     /translation="AEGSAMTPLLLKLIFDRIPGQLPAIVRPVGRMICTGVKKKVVEP
                     QLKNHFDYMENELVTHGPWFAGPEFTSADVQMSFPIEAGASRGGPDTERPKLKDFLER
                     IHARPAYQRALERGGPYQLLR"
     gene            complement(354..494)
                     /locus_tag="VGB85_08950"
     CDS             complement(354..494)
                     /locus_tag="VGB85_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134196.1"
                     /translation="MTHLEPRHFPPLAEIVGRAQYVQPARPEEPGLQTDLAPKQRHLS
                     SW"
     gene            620..>1670
                     /locus_tag="VGB85_08955"
     CDS             620..>1670
                     /locus_tag="VGB85_08955"
                     /inference="COORDINATES: protein motif:HMM:NF020076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEY0134197.1"
                     /translation="MMIRAALLSGITWTIACAPAQGSYQFLSPEPAETLVEVQSVWGP
                     VEGLAPEPIFVAASPPMIDDPVRDVVYTDPGELPRMHAGTMPLPLRHTAVRAHLRGTV
                     AEVVVKQRFVNGGELALEAIYTFPLPENSAVTDMRMVIGERVITSEIHERGEARRVYE
                     DARESGHRAALLEQERPNVFTQSIANIAAGEAIDVEIRYLQTLSYDAGEYEFVFPTVV
                     GPRYAPAGRVADAARINPPVLGRGQRSGHDLSIEVTAETGSAITGWAAPAHEVQATAV
                     GSQLQVKLARRGEIANRDFVLRYRSAAAQPAARLFLGPPGKTGNYFMLLAEPPRVDVD
                     ALVGSRELVFVVDVSG"
BASE COUNT          242 a          549 c          614 g          265 t
ORIGIN      
        1 gccgagggct cggcgatgac gccgttgctg ctcaagctga tcttcgatcg catcccgggc
       61 cagctgccgg cgatcgttcg cccggtcgga cgaatgatct gcaccggcgt caagaagaag
      121 gtcgtcgagc cgcagctcaa aaatcatttc gattatatgg agaacgagct ggtgacccac
      181 gggccgtggt tcgcggggcc ggaattcacc tcagcggatg tccagatgag cttcccgatc
      241 gaagccggcg cgtcgcgtgg cgggcccgac acggagcggc cgaagctcaa ggacttcctc
      301 gagcggatcc acgcgcggcc ggcgtatcag cgggcgctcg agcgtggcgg gccctaccag
      361 ctgctcaggt gacgctgttt cggggcgagg tcggtctgga ggccgggctc ctccggccgg
      421 gcgggctgta cgtattgcgc gcgcccgacg atctcggcga ggggtgggaa gtggcgcggc
      481 tcgaggtggg tcacgggatg gtcgcacgtg ctggtgctgc ggtacagcga cgacgtctgc
      541 gccccggatc gtgcgcgatc ggtcgaggtc cgatcgtggt cggtcgcgcc cgcctggtgt
      601 aggcggtaga gccgaagata tgatgatccg cgccgccttg ctctccggta tcacctggac
      661 gatcgcctgt gctccggcgc aggggtcgta tcagttcctg tcccccgaac ctgcggagac
      721 gctcgtggag gtccagtcgg tctggggccc ggtcgagggc ctcgcgcccg agccgatctt
      781 tgtcgccgcg agcccgccga tgatcgacga cccggtgcgc gacgtcgtct acaccgaccc
      841 gggcgagctg ccgcgcatgc acgccggcac catgccgctg ccgctgcggc acacggcggt
      901 gcgcgcccac ctgcgcggga cggtcgccga ggtggtcgtc aagcagcgct tcgtcaacgg
      961 cggcgagctg gcgctcgaag ccatttatac atttccgctg ccggagaaca gcgcggtgac
     1021 ggacatgcgg atggtgatcg gcgagcgcgt gatcacctcg gagatccacg agcgcggcga
     1081 ggcgcggcgg gtctacgagg acgcgcgcga gtcggggcac cgggcggcgc tgctcgagca
     1141 ggagcggccc aacgtgttca cccagtcgat cgccaacatc gcggccggcg aggcgatcga
     1201 cgtcgagatc cgctatctcc agacgttatc atatgacgcg ggcgagtatg aattcgtgtt
     1261 cccgacggtc gtcggcccgc gctacgcccc ggcggggcgg gtcgccgacg cggcgcgcat
     1321 caacccgcca gtgctcggac gcggccagcg cagcgggcac gacctgtcga tcgaggtgac
     1381 cgccgagacg ggctcggcga tcaccggctg ggccgcgccg gcgcacgagg tgcaggcgac
     1441 ggcggtcggc tcgcaattgc aggtcaagct cgcccgccgc ggcgagatcg ccaaccgtga
     1501 cttcgtgctg cgttatcgca gcgcggcggc ccagccggcc gcgcgcctgt tcctcgggcc
     1561 tcccgggaag accggcaatt acttcatgct gctggccgag ccgccgcgcg tcgacgtcga
     1621 cgcgctcgtg ggatcgcgcg agctggtgtt cgtcgtcgat gtgtcgggct
//
LOCUS       DASWNJ010000529         2178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_292646, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000529 DASWNJ010000000
VERSION     DASWNJ010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292646"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..827
                     /locus_tag="VGB85_08960"
     CDS             144..827
                     /locus_tag="VGB85_08960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0134198.1"
                     /translation="MTADRHDRHEQEDRHDAALLRAAAAGDPGAVTRLYAAHVDALYA
                     FAFYRAGRDPALAEDIVHDTLLDALRHAPQFEPSRGDLRAFLLMRLRNVARASLRDHH
                     RSHALATAWDQREATLVQIFQSLEGAPPGDELLARAETRELVHLAIAGLADNHRVALT
                     RKYLHGDTLEALGQRLGVSDDAAKSLLARARRAFRDAFAALRPDPASTTVASTGRPTP
                     VLREVRDVQ"
     gene            817..>2178
                     /locus_tag="VGB85_08965"
     CDS             817..>2178
                     /locus_tag="VGB85_08965"
                     /inference="COORDINATES: protein motif:HMM:NF020076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEY0134199.1"
                     /translation="MSNELPPEPGDETLDRLLVMTADPPQLDPDARARLLARLQRSRE
                     VDLSPAATPGRPRAPELPMSVPPPNRFRPALGYALAVAATGLLVWSTTSLLLPDVTPD
                     APDQKDSLAELGEVRNDAARPRLVTLADGSTLILREGAAFSQLGPRRLELLRGEALLE
                     VVPGSEPFSVTTTAGEVEVRGTKFLLRQDDTGLLASVLRGELRLVNPAGEATLQSGES
                     ANLAKGTAPVKRPAERLSHEVAWARDALADASDTHKAVRRGNLLGRFPTWNREWPLPV
                     RSMDVDVHVEDGVARTTIDQTFFNHSDYQLEGVYSFPLPADAAISRLAMYVDGKLMEA
                     GITERQEGREIYESIVYRRRDPALLEWMQGNEFRMRIFPLPARTEKRILLSYTQPLES
                     LYGDYSVRVPIPELDLPVGTLRYRIHVKDKTLSVDSCCVELTTRDEGEFRIAEATLKN
                     VPIG"
BASE COUNT          324 a          898 c          643 g          313 t
ORIGIN      
        1 cgcgccgctg catggtgatc gccgaccgcc gacctcctcc cctcgcctga cgcccctgga
       61 ccgcgcgata gcacccgaag aataattctt cgttcgtcgc gacacctcgc ccccggctcg
      121 cggacaatgc ctgccgaggg catgtgaccg ccgatcgaca tgatcgacat gaacaagaag
      181 atcgccacga cgccgcgctg ctgcgcgccg ccgctgccgg cgaccccggg gccgtcacgc
      241 gcctctacgc cgcccacgtc gacgccctct acgccttcgc cttctaccgc gccggccgtg
      301 accccgcgct cgccgaggac atcgtccacg acaccttgct cgatgcattg cgccacgctc
      361 cccagttcga gccgtcccgc ggcgacctgc gggcattttt attgatgcgc ctgcgcaacg
      421 tcgcccgcgc ctcgctgcgt gaccatcacc gcagccacgc gctcgcgacc gcgtgggacc
      481 agcgcgaggc cacgctggtc cagatcttcc agtccctcga gggcgcgccg cctggcgacg
      541 aattgctcgc gcgcgccgag acccgcgagc tcgtgcacct tgcgatcgcc ggcctcgccg
      601 acaaccatcg cgtcgcgctg acccgcaagt acctccacgg cgacacgctc gaggcgctcg
      661 gccagcgcct cggcgtctcc gacgacgccg ccaagtccct gctcgcgcgg gcgcggcgag
      721 cctttcgcga cgccttcgcc gcgctgcgcc ccgatcccgc gtccaccacc gtcgcgtcca
      781 ccggacgccc cacaccagtc ctccgggagg tgcgcgatgt ccaatgagct accccccgag
      841 cccggcgacg agaccctcga tcgcctcctc gtcatgaccg cagatccacc ccagctcgac
      901 ccggacgccc gcgcccgcct gctcgccagg ttgcagcgct cgcgtgaggt cgacctgtcg
      961 cccgccgcga cgcccggccg tccgcgtgct ccggagctcc ccatgtccgt cccgcccccg
     1021 aaccgcttcc gccccgccct cggctacgcc ctcgcggtcg ccgccaccgg cctgctcgtg
     1081 tggtccacca cctccctcct cctgcccgac gtcacccccg acgcacctga ccaaaaggac
     1141 agcctcgccg agctcggtga agtgcgcaac gacgccgccc gcccgcgcct cgtcaccctc
     1201 gccgacggct cgaccctgat cctccgcgag ggcgccgcct tttcgcagct cggaccgcga
     1261 cgcctcgagc tcctgcgcgg cgaggccctg ctcgaggtcg tgcccggcag cgagcccttc
     1321 tccgtcacca ccacggccgg cgaggtcgag gtccgcggca ccaagttcct gctgcgccag
     1381 gacgacaccg gcctgctcgc cagcgtgctg cgcggcgagc tgcgcctcgt caaccccgcc
     1441 ggcgaggcga ccctgcagtc cggcgagtct gccaacctgg ccaaagggac agcccctgtc
     1501 aagcgtccgg ccgagcgcct cagccacgag gtcgcgtggg cgcgcgacgc cctcgccgac
     1561 gccagcgaca cccacaaggc cgtgcgacgg ggaaacctcc tcggtcggtt cccgacctgg
     1621 aaccgcgagt ggccgctgcc ggtgcgcagc atggacgtcg acgtgcacgt cgaggacggc
     1681 gtcgcccgca cgacgatcga ccagaccttt tttaatcaca gcgattacca gctcgagggc
     1741 gtgtacagct tcccgctgcc ggccgacgct gcgatctcgc gcctggcgat gtacgtcgac
     1801 ggcaagctga tggaggccgg catcaccgag cgccaggagg gccgcgagat ctacgagtcg
     1861 atcgtatatc gccgccgcga tcccgcgctg ctcgagtgga tgcaaggcaa tgagtttcgc
     1921 atgcggatct tccccctgcc cgccaggacc gagaagcgga tcctgctgag ctacacccag
     1981 ccgctcgaga gcctgtacgg cgactacagc gtgcgcgtcc cgatccccga gctcgacctc
     2041 ccggtcggca ccctgcgcta ccggatccac gtcaaggaca aaaccctctc cgtcgactcc
     2101 tgctgcgtcg agctgaccac ccgcgacgag ggcgagtttc gcatcgccga ggccacttta
     2161 aaaaatgtcc cgatcggt
//
LOCUS       DASWNJ010000530         2248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_609525, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000530 DASWNJ010000000
VERSION     DASWNJ010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609525"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1390
                     /locus_tag="VGB85_08970"
     CDS             <1..1390
                     /locus_tag="VGB85_08970"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEY0134200.1"
                     /translation="LASASLLPQTERMLFEDMRSEQLEAEREVEKLSTRYGEKMVELQ
                     QARGRLKLIEARIAREERELLESLKARANAATSTERQLRESLETEKNRALELTHLERE
                     YRELEREATTAAETYALVARRDTEIEMTNRVEAQDIEVLDRATRPREPVFPPKALLVA
                     IALVGGLTLGALLALGVDVRDHRIRGLLDLERAIAGFGLPVLGQLPLLPAEARLGVGN
                     IRAQRRHRDLHTLLFPQSLMAERCRAIRTSLSFVQGESVLHTLVVTSPNSNEGKSSTA
                     INLAMSFAQAKKRVILVDADMRRPRLHQVFPAPIGKEVVGLAGVLSGRCSLDEAILTT
                     NEDLPDNLHLLLCGEIPQNPAELLDSPQCRKLLAELSERYDVAILDSPPVLPVTDPLI
                     LARLAHGLIVVARCQATTRSELQRTLTLLRQGDNNLLGVILNEVDTRREEAGYGVGYY
                     AYHARETSPEQA"
     gene            1472..>2248
                     /locus_tag="VGB85_08975"
     CDS             1472..>2248
                     /locus_tag="VGB85_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134201.1"
                     /translation="MPADTHPRTPADGTPTAAGAPADGEGTSRARTPESEATNPAAQP
                     DAAASGDIAADSKPGAQPGAPASPLPGRLSGAVPSIVVHRPGSTHHVAKASRVLSAPL
                     PRAPAVDEAEEPLASVTLGERPGQPPWARMALALAICGPALWLGGVPVVVVPVFLGVT
                     LLLWLRLCLRSPGPIRVPHGSIIGLIAATMTLLQWLPLPRALREWLAPDLVAMSTAAL
                     AGSGVGPWAGVSPVPGDTAVEAARLFGLTALYVAAAQLSWR"
BASE COUNT          313 a          862 c          779 g          294 t
ORIGIN      
        1 cctcgccagc gccagcctgc tgccgcagac cgagcgcatg ctgttcgagg acatgcgcag
       61 cgagcagctc gaggccgagc gcgaggtcga gaagctgagc acccgctacg gcgagaagat
      121 ggtcgagctg cagcaggcgc gcggccgcct caagctgatc gaggcgcgca tcgcccggga
      181 ggagcgcgag ctgctcgagt cgctcaaggc ccgcgccaac gcggcgacct cgaccgagcg
      241 ccagctgcgt gagtcgctgg agaccgagaa gaaccgcgcg ctcgagctga cgcacctcga
      301 gcgcgagtat cgcgagctcg agcgcgaggc gacgaccgcc gccgagacct acgcgctggt
      361 cgcccgccgc gacaccgaga tcgagatgac caaccgggtc gaggcgcagg acatcgaggt
      421 cctcgaccgg gcgacgcggc cgcgcgagcc ggtgttcccg ccgaaggcct tgctggtggc
      481 gatcgcgctg gtcggcggct tgacgctcgg cgccttgctg gcgctcggcg tcgacgtccg
      541 cgaccaccgg atccgcggcc tcctcgacct cgagcgggcg atcgccggct tcggcctgcc
      601 ggtgctcggg cagctgccgc tgttgcccgc ggaggcgcgg ctcggcgtcg gcaacatccg
      661 cgcccagcgg cgccaccgcg acctgcatac gctgctgttc ccgcagtcgc tgatggccga
      721 gcgctgccgt gcgatccgga cctcgctgag cttcgtgcag ggcgagtcgg tgttgcacac
      781 gctcgtcgtc acctcgccga actcgaacga gggcaagagc tcgacggcga tcaacctggc
      841 gatgagcttc gcgcaggcca agaagcgggt gatcctcgtc gacgccgaca tgcggcggcc
      901 gcgtctgcat caggtgttcc ccgcgccgat cggcaaggag gtcgtcggcc tcgccggcgt
      961 gctgtccgga cgttgcagcc tcgacgaggc tatcctcacc accaacgagg atctgcccga
     1021 caacctccac ctgctgctgt gtggggagat cccgcaaaac ccggccgagc tgctcgacag
     1081 cccgcagtgc cgcaagctgc tcgccgagct cagcgagcgc tacgacgtcg ccatcctcga
     1141 ctcgccgccg gtcctcccgg tcaccgaccc gctcatcctc gcccgcctcg cccacggcct
     1201 gatcgtcgtc gcccgctgcc aggcgaccac gcgcagcgag ctgcaacgca ccctcacgct
     1261 gctgcgccag ggcgataaca acctcctcgg cgtcatcctc aacgaggtcg atacgcggcg
     1321 ggaagaagcg ggatacggcg tcgggtacta tgcttaccac gcgcgtgaga ccagccccga
     1381 gcaggcctga accgtcggcc tgaccaccgc cgttgcgaac ggacgacgtg gcacccagcc
     1441 gacgggccgg ctcaccccgc gcactccccc gatgcccgca gacacgcacc cccggacccc
     1501 ggccgacggg acgccgaccg ccgcaggcgc ccccgccgat ggtgagggga cgtcgcgcgc
     1561 acgaacgccg gagagcgagg cgacgaaccc ggccgcgcag cccgacgcgg ccgccagcgg
     1621 cgacatcgcg gcggattcca agcccggcgc gcagcccggt gcgccggcct ctccgctgcc
     1681 gggccggctc agcggggctg tcccttcgat cgtggtccat cggccaggtt ccacgcacca
     1741 tgtcgcaaag gccagccggg tgctgagcgc gccgctgcca cgcgctccgg cggtcgacga
     1801 ggcggaggag ccgctggcga gcgtgacgct cggcgagcgc cccggtcagc cgccgtgggc
     1861 ccgcatggcg ctcgcgctgg cgatctgcgg tccggccctg tggctcggcg gcgtgcccgt
     1921 cgtcgtcgtg ccggtgttcc tcggcgtcac gttattgctg tggctgcgcc tgtgcctgcg
     1981 cagccccggc ccgatccgcg tgccccacgg cagcatcatc ggcctgatcg ccgcgacgat
     2041 gacgctgctc cagtggctcc cgctcccgcg agcgctgcgc gagtggctcg cgcccgacct
     2101 cgtcgccatg tccaccgcgg cgctcgcggg cagcggcgtg ggcccgtggg ccggcgtctc
     2161 accggtgccc ggcgacaccg ccgtcgaggc cgcgcggctg ttcggcctga ccgcgctgta
     2221 cgtcgccgcc gcccagctgt cgtggcgc
//
LOCUS       DASWNJ010000531         1732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_150870, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000531 DASWNJ010000000
VERSION     DASWNJ010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150870"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="VGB85_08980"
     CDS             complement(<1..1009)
                     /locus_tag="VGB85_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134202.1"
                     /translation="MAHSPEKLLQSIPGLVAAKDAAALVAIQDHDDKQVRKAARKAIH
                     TLRAKGVEIPSAEAPRSWVAASPQSLRGEQHEAAMLDASSTPGLVRLMIAAPQAEERG
                     YLWVAALTGRDQVAEFTAYLQTDGQRSRLVRDWARDFKGRQVPADWARARIRWAREQT
                     LSSGFSVPNQLDDMLVHLGPAPTSRPASFLIGQLPADFKGERDNVEVALTSARVFAWP
                     PLFDVEPTLKKVNEANPNMSAETPEAERYDALMTAVAGDESVRSALQGSVANLLEDAA
                     AGLWLQGADGPAAQVLALTQELRTSAAPEALPWIGRLLGFQIATTLQYMMRQQQQQQQ
                     QR"
     gene            complement(1026..>1732)
                     /locus_tag="VGB85_08985"
     CDS             complement(1026..>1732)
                     /locus_tag="VGB85_08985"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY0134203.1"
                     /translation="LTHLWLGRVRTGAIVPDTAQREYIRRLYRGELQRVDRGLGMLLD
                     ALSTDGTLDEAIVVLVGVHGEEFFEHGGVGHGVTLHDESLRVPLAIRAPALLAPGRVS
                     VPVDLLDLAPTLADLLGLPFPGAWQGDTLVPVIDDPQPPPRLVVAYLGDGSRAAIVGD
                     LKLILGSGRDSQRFYDLAADPGELDDRLATGGIGLRIVRSALAWELAAPGQTWKRARW
                     GTGANLRPAFAVDHGM"
BASE COUNT          231 a          586 c          678 g          237 t
ORIGIN      
        1 ggcgctgctg ttgctgctgc tgctgctggc gcatcatgta ctgcagcgtg gtcgcgatct
       61 ggaagccgag caggcgaccg atccacggca gggcctccgg cgcggcgctg gtgcgcagct
      121 cctgcgtgag cgcgagcacc tgcgcggccg ggccgtcggc gccttgcagc cacaggccgg
      181 cggcggcgtc ctcgagcagg ttggcgaccg agccctgcag ggccgagcgc acgctctcgt
      241 cgccggcgac cgcggtcatc agcgcgtcgt agcgctcggc ctccggggtc tcggcgctca
      301 tgttcgggtt ggcctcgttg accttcttga gcgtcggctc gacgtcgaac agcggcggcc
      361 aggcgaacac gcgggccgag gtcagcgcga cctcgacgtt gtcgcgctcg cccttgaagt
      421 ccgccggcag ctgcccgatg aggaagctgg cggggcgcga ggtcggggcc gggccgaggt
      481 gcacgagcat gtcgtcgagc tggttgggca cgctaaagcc ggacgagagg gtctgctcgc
      541 gggcccagcg gatgcgggcg cgggcccagt cggcggggac ctggcggccc ttgaagtcgc
      601 gggcccagtc gcggaccagg cgcgagcgct ggccgtcggt ctgcaggtag gcggtgaact
      661 cggcgacctg gtcgcggccg gtgagcgcgg cgacccacag gtacccgcgc tcctcggcct
      721 gcggcgcggc gatcatcagg cgcacgaggc cgggggtcga cgaggcgtcg agcatcgcgg
      781 cctcgtgttg ttcaccgcgc agcgactgcg ggctcgcggc gacccagctg cgcggagcct
      841 cggcgctggg gatctcgacc cccttcgcgc gcagcgtgtg gatcgccttg cgcgcggcct
      901 tgcggacctg cttgtcgtcg tggtcctgaa ttgcgacgag ggccgcggca tccttggcag
      961 cgacgagacc ggggatcgac tggaggagct tttcggggct gtgggccatg gaagccgcag
     1021 ccttactaca tcccatggtc gaccgcaaag gccggccgca ggttggcgcc ggtgccccag
     1081 cgggcccgtt tccaggtctg accgggggcc gcgagctccc aggcgagggc gctgcggacg
     1141 atccgcaggc cgataccgcc agtcgccagg cggtcgtcga gctcgccagg gtcggccgcg
     1201 aggtcgtaga agcgctggga gtcgcgtccg ctgccgagga tgagcttgag atcgccgacg
     1261 atcgccgcgc gcgagccatc gccgaggtag gcgacgacca gccgcggcgg cggctgcggg
     1321 tcgtcgatca cgggcacgag cgtgtcgccc tgccaggcgc ccggaaacgg caggccgagc
     1381 aggtcggcga gggtcggcgc gaggtcgagg agatcgaccg gcaccgacac gcgcccgggc
     1441 gccagcaagg ccggggcgcg gatcgccagc ggcacgcgca ggctctcgtc gtgcagcgtg
     1501 acgccgtgac cgacgccgcc gtgctcgaag aactcctcgc cgtgcacgcc gacgagcaca
     1561 acgatcgcct cgtcgagcgt gccgtcggtg gacagcgcgt ccagcaacat gccgaggccg
     1621 cggtcgacgc gttgcagctc gccgcgatag aggcggcgga tgtactcgcg ctgggccgtg
     1681 tccgggacga tcgcgccggt gcgcacgcgc ccgagccaga ggtgcgtcag at
//
LOCUS       DASWNJ010000532         2648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_434018, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000532 DASWNJ010000000
VERSION     DASWNJ010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434018"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..320)
                     /locus_tag="VGB85_08990"
     CDS             complement(54..320)
                     /locus_tag="VGB85_08990"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEY0134204.1"
                     /translation="MNVDLSQLGDVVARLNRVQGQIGGVVRMIEQGEDCAAVVTQLAA
                     ASKALDRAGFKIISTGMQQCLTQDDTGERKVDVAQLEKLFMSLA"
     gene            561..1910
                     /locus_tag="VGB85_08995"
     CDS             561..1910
                     /locus_tag="VGB85_08995"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0134205.1"
                     /translation="MISTQHVAARFPPEPVSARAARRFLRDFLKRCGRIDLADDAELA
                     LSEVVTNAVLHAHTPIDVALSLDRDGALRAEVFDRNPSLPAQRHYAEQATTGRGMQLI
                     AALTAECGVEAGDPDGKVVWFIVRGDSSRSPGLGTDDVSPGWDAPAVVDAPATTGASV
                     MLLGLPPTLWLAAREHHDALLRELGLFAQEHHGNAPSPERFVQADLARGAVSTRVVAE
                     LERLAASVQAHRALPSGHPSPLPDTPSELDLEVRVPEDAAAAFGALQDVLDAAERLAV
                     AGQLLARPGLPEVVAVRDWVCEQVIAQAAGVSPSRWAGTGQERFTVDVHDRSGPAVPD
                     WDAAVVVGSQRGAVAADDANRIIAVSRPLADALGWDVDELVGRRVVALIPPELREAHV
                     AGFTRHLTTGEAHVLGVPLRLPVLRADGTRLECDFLIEQAPATTGRAVYIAWIDPIA"
     gene            2170..2484
                     /locus_tag="VGB85_09000"
     CDS             2170..2484
                     /locus_tag="VGB85_09000"
                     /inference="COORDINATES: protein motif:HMM:NF044515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134206.1"
                     /translation="MEAVLRAWARIARESRGHRDAELPAVLHRDGGVDRAERHLHPLA
                     ASLMEQAAADGDMRADVPPGELAAICLHALTAAAGMPADRAADRLVGVALAGLRPAPH
                     PS"
BASE COUNT          328 a          988 c          960 g          372 t
ORIGIN      
        1 ctggaccagg ccgccgtggg tcaggcatgc gtcgtcgtgc ggggggagcg ccgtcaggcg
       61 agggacatga agagcttctc gagctgcgcg acgtcgacct tgcgttcacc cgtgtcgtcc
      121 tgcgtgaggc actgctgcat gcccgtcgag atgatcttga agccggcacg gtccagcgcc
      181 ttggacgcgg cggccagctg ggtgacgacc gcggcgcagt cctcgccctg ctcgatcatc
      241 cggacgacac cgccgatctg gccctgcacg cggttgagcc gcgcgacgac gtcgccgagt
      301 tggctgaggt ccacgttcat ctcgatctcc cttcggggta ccccctgggg tacagggtaa
      361 gcgtacgtca gatgcttccc ctcggcatgg ggcggctcct cggatgcccc ctcgggtgat
      421 ggctgcgacc tccagacgaa ggatggcagt cgtacgcggg cgccggcact gctcggcaag
      481 gccctggtcc cgtgggagca aatcgggcat gctgacaggg tgcgccacac gccgtaccgg
      541 ccccctgcgc ccgcccgagc gtgatcagca cgcagcacgt ggcggcgagg ttcccgcccg
      601 agccggtgag cgcccgcgcc gcccgccgct tcctgcgcga cttcctgaag cggtgcgggc
      661 gcatcgatct ggccgatgac gccgagctcg cgctgtcaga ggtcgtgacg aacgccgtcc
      721 tgcacgcgca cacgcccatc gacgtggcgc tgtcgctcga ccgggacggc gcgctgcggg
      781 ccgaggtgtt cgaccgcaac ccgtcgctgc cagcgcagcg ccactatgcc gagcaggcca
      841 cgaccggacg cggcatgcaa ctgatcgccg cgctcaccgc ggagtgcggc gtcgaggccg
      901 gtgaccctga cgggaaggtg gtgtggttca tcgtccgcgg cgacagcagc cgcagccccg
      961 ggctcggcac ggacgatgtg tctccagggt gggacgcgcc cgcggtcgtc gacgcacccg
     1021 cgacgactgg cgcgtccgtc atgctgctcg ggctgccgcc gacgctgtgg ctcgccgctc
     1081 gcgagcacca cgacgcgctc ctgcgcgagc tcggcctgtt cgcgcaggag caccacggca
     1141 atgcgccgtc cccggagcgc ttcgtgcaag ccgacctggc ccgcggcgcg gtctccacac
     1201 gggtcgtggc ggagctggaa cggctggccg cgtcggtcca ggcgcaccgc gccttgccat
     1261 ccgggcaccc gagcccgctg cccgacacgc cgtccgagct cgaccttgag gtcagggtcc
     1321 ccgaggacgc cgcggcggcc ttcggggccc tgcaggacgt tctcgacgcc gcggagcgcc
     1381 tcgccgtcgc tgggcagctg ctggcacggc cggggctgcc cgaggtcgtc gcggtccgcg
     1441 actgggtgtg cgagcaggtc atcgcccagg cggccggtgt cagcccgtcg cggtgggccg
     1501 gcaccggcca ggagcgcttc accgtggacg tgcacgaccg ctccgggcct gctgtgccgg
     1561 actgggacgc ggcggtggtc gtcggctctc agcgcggcgc cgtcgccgcc gacgacgcga
     1621 accggatcat cgccgtcagc cgcccccttg ccgacgcgct cggctgggac gtcgacgagc
     1681 tggtcggacg acgcgtcgtc gccttgatcc cgcccgagct gcgcgaggcc cacgtcgccg
     1741 gtttcacgcg gcacctcacg accggcgagg ctcacgtcct cggcgtgccg ctgcgcctgc
     1801 cggtgctgcg ggccgacggc acacggttgg agtgcgactt cctcatcgag caggctcctg
     1861 cgacgactgg tcgcgcggtc tacatcgcct ggatcgaccc gatcgcctga tcaccggcgg
     1921 gacgaccatc ggctgctgcc cgtggccgtc agtgactgca gacgtcgaca cccatgccgc
     1981 cgccggcttt cgtgcggccc cccgtcgacg actcgatcgc ccatgcgacc gacacgcacg
     2041 cgctgacgcg ctggctcaca gcctcgtgtc gacggcaccg ccgcatgcag gctggcgcag
     2101 tccctgacgt tgtcgacccg agtctcgtcg gagtagccga gccatctcgt aggccccgcc
     2161 gccgcgcgac tggaggcggt cctccgcgcg tgggcgcgaa tcgcgcggga gagccggggc
     2221 caccgtgacg cggagctccc ggcggtgctc caccgcgacg gcggcgtcga ccgggccgag
     2281 cgccacctgc accccctcgc cgcgtcgctg atggagcagg ccgccgccga cggggacatg
     2341 cgcgccgacg tgccgcccgg cgagctcgcc gcaatctgcc tgcacgcgct gaccgcggca
     2401 gcgggcatgc ccgccgaccg ggcggcggac cggctcgtcg gcgtggcgct ggccggcctg
     2461 cgccctgccc cgcacccctc gtagaggcgc ttccggacgg cggaggcagt gtcgcgcggg
     2521 cggcgcgggc cggctcaggc cttgtacgcc cagtgccagg gctcgcgcgg ggtgtcctcg
     2581 atgaagccga accgtgggcc gttcgcgcgc atccaggcct gtgcgcgggc gtcgaggtcg
     2641 aggtcgac
//
LOCUS       DASWNJ010000533         2058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_679746, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000533 DASWNJ010000000
VERSION     DASWNJ010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679746"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VGB85_09005"
     CDS             complement(<1..718)
                     /locus_tag="VGB85_09005"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY0134207.1"
                     /translation="MNNLPPPPTNKPSSLFGTPSSMLGSRPGSAPQHAAETNAPAALG
                     VGKITQVIGPVVDVEFPAGLPEIFTALKVTNGRISDQPDNLTLEVALHLGERTVRAIA
                     MDTTDGLVRGMDVRDTGKPIQMPVGKGVLGRIMNVIGEPVDEQGPLMTTETRSIHRAA
                     PTFMEQSTKVEVLVTGIKVIDLLAPYRRGGKIGLFGGAGVGKTVLLMELVNNVGKKFG
                     GYSVFAGVGERTREGNDLYFE"
     gene            complement(781..1683)
                     /gene="atpG"
                     /locus_tag="VGB85_09010"
     CDS             complement(781..1683)
                     /gene="atpG"
                     /locus_tag="VGB85_09010"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEY0134208.1"
                     /translation="MANLKEIRGRIASVKNTRKITSAMSRIAAARLVKAQQAALGARP
                     YGERLEEVVRALVAGAAECEVLHPLMAPRTGNGRVGLVLMTADRGLAGGFNANANRAG
                     LNFIRRERGEGREVVVIAVGKKGRSFLGSNRTSIERFHEAPTLATLVQRSKEVAAEVM
                     ALFEPAAEGQTGLPQCDAVYLTYNYFKSVLTQEPRTVQILPVKPPEASASGDQPVAPL
                     FEPDRASLLGHLLPIALEAQLQQAMFNSIAGEIAARRTAMDSATDNATQLIQELTRHY
                     NRERQAAITKELMEIIGGAEALKG"
     gene            complement(1694..>2058)
                     /locus_tag="VGB85_09015"
     CDS             complement(1694..>2058)
                     /locus_tag="VGB85_09015"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970813.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEY0134209.1"
                     /translation="AFSQFASDLDKATRDTLARGQRLTELLKQAQYVPLHVALQVVSI
                     YAGTRGHLDELDLGEVRSFEVKLHAWLRDNRADLLELIDRASAKPDLLKVDEGLESAI
                     KAFKEIYLKDPARKQAKA"
BASE COUNT          274 a          668 c          767 g          349 t
ORIGIN      
        1 tctcgaagta caggtcgttg ccctcgcggg tgcgctcgcc gacgccggcg aacacggagt
       61 agccgccgaa cttcttgccg acgttgttga ccagctccat caggagcacg gtcttgccga
      121 cgccggcgcc gccgaacagg ccgatcttgc cgccgcgacg gtagggcgcg agcaggtcga
      181 tgaccttgat gccggtgacg agcacctcga ccttggtcga ctgctccatg aaggtcggcg
      241 cggcgcggtg gatcgaccgc gtctcggtgg tcatcagcgg gccctgctcg tcgaccggct
      301 caccgatgac gttcatgatg cggccgagca cgcccttgcc gaccggcatc tggatcggct
      361 tgccggtgtc gcgcacgtcc atgccgcgga ccaggccgtc cgtcgtgtcc atggcgatcg
      421 cacgcacggt tctttcgccg aggtgcagcg cgacctcgag ggtcaggttg tccggctggt
      481 cgctgatgcg gccgttggtc accttgagcg ccgtgaagat ctcgggcagg cccgccggga
      541 actcgacgtc gacgaccggg ccgatgacct gggtgatctt gccgacgccc agggctgcgg
      601 gggcgttcgt ctcggccgcg tgctgcggcg cggagccggg gcggctcccg agcatcgagc
      661 tgggcgtacc gaagagagag ctgggcttgt tggtgggcgg aggcggcagg ttgttcatgg
      721 ggctccgaag ctacgggagg gacagagaac tcggggcgct cagggaagat cgaggtgccg
      781 ctagcccttg agggcctcgg cgccgccgat gatctccatg agctccttgg tgatcgcggc
      841 ctgacgctcg cggttgtagt ggcgagtcag ctcctggatg agctgggtcg cgttgtcggt
      901 cgccgagtcc atcgcggtcc ggcgggcggc gatctcaccg gcgatcgagt tgaacatcgc
      961 ctgctgcagc tgcgcctcga gggcgatcgg cagcaggtga ccgagcaggc tcgcgcggtc
     1021 gggctcgaac agcggcgcga ccggctggtc gccgctcgcg ctggcctcgg gcggcttgac
     1081 gggcaggatc tgcaccgtgc gcggctcctg ggtcagcacg ctcttgaagt agttgtaggt
     1141 caggtagacc gcgtcgcact gcggcagccc ggtctgcccc tcggcggccg gctcgaacag
     1201 cgccatcacc tcggcggcga cctccttcga gcgctgcacc agcgtggcga gcgtcggcgc
     1261 ctcgtgaaac ctctcgatag aggtccggtt cgagccgagg aacgagcggc ccttcttgcc
     1321 gacggcgatg acgacgacct cacggccctc gccgcgctcg cggcggatga agttgaggcc
     1381 cgcgcggttg gcgttggcgt tgaagccacc ggcgaggccg cggtcggccg tcatcaggac
     1441 caggccgacg cggccgttgc cggtgcgtgg ggccatcagc gggtgcagga cctcgcactc
     1501 ggccgcgccg gcgacgaggg cgcggacgac ctcctcgagg cgctcgccgt agggacgggc
     1561 cccgagcgcg gcctgctggg ccttgacgag gcgcgccgcg gcgatccggc tcatcgccga
     1621 ggtgatcttg cgggtgttct tgaccgaggc gatacgcccg cggatctcct tcaggttggc
     1681 catggcttgc tcctcaggcc ttcgcctgct tgcgcgccgg atccttgagg tagatctcct
     1741 tgaacgcctt gatcgcgctc tcgaggccct cgtcgacctt gagcaggtcc ggcttggcgg
     1801 aggcgcggtc gatcagctcg agcaggtcgg cgcggttgtc ccgcagccac gcgtgcagct
     1861 tgacctcgaa ggagcggacc tcgccgaggt cgagctcgtc gaggtggccg cgggtgccgg
     1921 cgtagatgct gacgacctgc agggcgacgt gcagcggcac gtactgggcc tgcttgagca
     1981 gctcggtcag gcgctgtccg cgggcgagcg tgtcgcgggt cgccttgtcg aggtccgagg
     2041 cgaactgcga gaaggcct
//
LOCUS       DASWNJ010000534         2312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_44879, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000534 DASWNJ010000000
VERSION     DASWNJ010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44879"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VGB85_09020"
     CDS             complement(<1..209)
                     /locus_tag="VGB85_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134210.1"
                     /translation="MANQSFTASIRMTAMSGKRLARPKARGFLPAHLDGDKPRCCELY
                     DCLDDTDSETDKPLKPSVAEAAGKT"
     gene            complement(337..1029)
                     /locus_tag="VGB85_09025"
     CDS             complement(337..1029)
                     /locus_tag="VGB85_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134211.1"
                     /translation="MTSTRQPYRPRHAGIASIFTSVTIVGLVLMFGCGRRELRDLERA
                     SDTAAAAVRRGDARALSKQVLVGARNRVDYAAILADKQRRADWVRRLSKPDAVRPEAT
                     LFVASDRPLAVVWTGQRWAFAEDPTLVYDQSSPRAALRSLVRASTMERWDVLLGLAPE
                     RYRLGLGEDDLRRAWTEGPYATALGTSRDRLREHLADPIVSDAHEAVLDLGDGEAAHL
                     EREGTRWVVVDF"
     gene            complement(1026..1523)
                     /gene="fabZ"
                     /locus_tag="VGB85_09030"
     CDS             complement(1026..1523)
                     /gene="fabZ"
                     /locus_tag="VGB85_09030"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF000582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEY0134212.1"
                     /translation="MLLDAVQIQKILPHRFPFLFLDRVISAVPGESIVAQKLVSMSDP
                     ILQGHFPGNPILPGVVQVEAMAQAAVVLAHLSGLFDLELHHCYFIGIQEAKFRAPVVP
                     GEVLTIEVKAQRLGRLGKFSGEIRAGDQLKSSGTFTAIIEPKIKPAPAVVPASTPAPV
                     TDPSA"
     gene            complement(1616..2173)
                     /locus_tag="VGB85_09035"
     CDS             complement(1616..2173)
                     /locus_tag="VGB85_09035"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEY0134213.1"
                     /translation="MTTSSTVRRLLTASALALGVATASLAPVLTAPAQAAVPKVRGVA
                     VVDMQKVLNDTKQGVSARKKLEDSSKAKQEKLDGKRKKLEADQAKLKNLSGDKLAQAE
                     ETLQKEYMEMQQIYMTMQQELAAQEGRVLEDIYKNCQSVIDKIAAEMEIDLVLIRDET
                     TVLFAEPGLDITDVLVKRYNAKYPS"
BASE COUNT          344 a          717 c          849 g          402 t
ORIGIN      
        1 atggtcttgc ctgccgcttc tgccaccgag ggcttcaagg gtttgtcagt ctccgagtcg
       61 gtgtcgtcga ggcagtcgta aagctcacag cacctgggct tgtctccgtc gaggtgagca
      121 ggtaggaatc ccctcgcctt cggccgggcc agcctcttcc ctgacattgc tgtcatgcgg
      181 atcgaggcag tgaaactttg gttggccacc ttaccgaaca gactaacgcc catggacgcg
      241 atggcaagaa ctgtcttcgc gcccgtcgca gtgcgctttg tgcccacacc tcatgaggcg
      301 atggcgatgg aagcgatggc gcgaggcgac ctccggtcag aagtcgacga cgacccagcg
      361 cgtaccctcg cgctcgagat gggccgcctc gccgtcgccc agatcgagga ccgcctcgtg
      421 cgcgtcgctg acgatcgggt cggcgaggtg ctcgcgcagg cggtcgcggc tggtccctag
      481 cgcggtcgcg taaggcccct cggtccacgc gcggcgcagg tcgtcctcgc cgagaccgag
      541 gcgatagcgc tccggagcga ggcccaggag cacatcccag cgctccatcg tgctcgcgcg
      601 gacgaggctg cgcagggcgg cgcgcgggct cgactggtcg tagaccagcg tcggatcctc
      661 ggcgaacgcc cagcgctggc cggtccatac gacggcgagc gggcggtcgc tggcgacgaa
      721 gagggtcgcc tccgggcgca ccgcgtcggg cttcgagagg cggcggaccc agtcggcgcg
      781 gcgctgtttg tcggcgagga tcgcggcgta atcgacccgg ttgcgggccc cgacgagcac
      841 ctgcttgctc agcgcgcggg cgtcgccgcg acgcaccgca gccgccgccg tgtcgctcgc
      901 tcgctcgagg tctcgcagct cgcgacgccc gcagccgaac atcagcacga gaccgacgat
      961 cgtgacggac gtgaagatcg aggcgatccc ggcgtgccgc ggtcggtacg gttggcgcgt
     1021 ggacgtcatg cggacgggtc ggtcaccgga gcgggcgtgg aggcgggaac gacggcgggc
     1081 gcgggcttga tcttgggctc gatgatcgcg gtgaaggtgc cggagctctt gagctggtcg
     1141 ccggcgcgga tctccccgga gaacttgccg aggcgtccga ggcgctgggc cttcacctcg
     1201 atcgtgagga cctcgcccgg gacgacgggg gcgcggaact tggcctcctg gatcccgatg
     1261 aaatagcagt ggtgcagctc gaggtcgaac aggccggaca ggtgggcgag caccaccgcg
     1321 gcctgcgcca tcgcctcgac ctggaccacg ccgggcagga tcgggttgcc ggggaagtgt
     1381 ccctggagga tcgggtcgct catcgacacc agcttctgcg ccacgatcga ctcgccgggc
     1441 accgccgaga tcacgcggtc gaggaagagg aacgggaagc gatgcgggag gatcttctgg
     1501 atctggacgg cgtcgaggag catgcgggcc ggcagagcat aaacgacttc gccgcggacg
     1561 aggagtgctc gccgcggcgg tgcgccatcg tagggccaga accccgtacg gggctttagc
     1621 tcgggtactt ggcgttgtag cgcttgacca ggacgtcggt gatgtcgagg ccgggctcgg
     1681 cgaacaggac ggtggtctcg tcgcggataa ggacgaggtc gatctccatc tcggccgcga
     1741 tcttgtcgat gacgctctgg cagttcttgt agatatcctc gagcacgcgg ccctcctggg
     1801 cggccagctc ctgctgcatg gtcatgtaga tctgctgcat ctccatgtac tccttttgca
     1861 gcgtctcctc ggcctgggcg agcttgtcgc cgctgaggtt cttgagcttg gcctgatcgg
     1921 cctcgagctt cttgcgcttg ccgtcgagct tctcctgctt ggccttggag ctgtcctcga
     1981 gcttcttgcg ggccgagact ccctgcttgg tgtcgttgag gaccttttgc atgtcgacga
     2041 cggcgacgcc gcggaccttg ggcacggcgg cctgcgccgg ggcggtcagg acgggcgcga
     2101 ggctggcggt ggcgaccccg agagcgaggg cggaggcggt aagcaggcga cgaacagtgg
     2161 acgaggtggt catggcgggc atgggaggca tctcgggctc aggggtgctt cttgtcgtag
     2221 gcgacgatga tgcgattggt gaggtcgagc ttcgggttgg cgtagagcac gttggcctgc
     2281 gagcggacca gcacgatctg cagcgtctct tc
//
LOCUS       DASWNJ010000535         5150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_292718, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000535 DASWNJ010000000
VERSION     DASWNJ010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292718"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1516
                     /locus_tag="VGB85_09040"
     CDS             <1..1516
                     /locus_tag="VGB85_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134214.1"
                     /translation="EPFREQRLEQLKALAAGRGGACLDEVYFRKRHPMRWRCQHGHEW
                     EAQSDSVLRGNWCPFCSGRRTTVAALQALAAERGGEFLSTEVGRKIDPHRWRCAHGHE
                     WPAAPSKIIEGTWCPTCAGRNTITLATLQELARSRGGACLAQQYTSINQHVRWRCDAG
                     HEWSATANSTRRGTWCPTCAGNSRGSLSRLREVARERGGKCLASAYINSRTPVPWRCA
                     EGHTWSAAPSNVVAGGWCRECSYHIRRSKRPTLADLVARCREQAGRVLLDLPDDTVIH
                     GDTRATFQCVMGHTWTTAVHTVRSGHWCPECAGVARGGLAALQAIAAERGGRCLATEY
                     IDNRTPIPFECAEGHRWNARPFGIKRTWCPQCAPTRRLDLTMLRADASARGGRCLADV
                     LPGRDQPIAWACAQGHQWMARPTDVHGGSWCPKCRGVGTYTLERARKAAARHGGECLS
                     TACARATDVLSWRCREGHTWTSAAMYVLRIGTWCPRCGRERGAAKLRESFSATG"
     gene            2002..2214
                     /locus_tag="VGB85_09045"
     CDS             2002..2214
                     /locus_tag="VGB85_09045"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0134215.1"
                     /translation="MKPTVPPITLEDFNLEATERRLCVEALRLAGNIVGAAEILGITR
                     HALKRRIIKLDIPWSRNGSIDPDTST"
     gene            complement(2229..2441)
                     /locus_tag="VGB85_09050"
     CDS             complement(2229..2441)
                     /locus_tag="VGB85_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134216.1"
                     /translation="MRHDRLHLGQLVVQREAEPRLRVHLANVEHGLADALDWQVEATI
                     DFAALAGQQIYGLGDPRLDALLDELE"
     gene            complement(2503..4614)
                     /locus_tag="VGB85_09055"
     CDS             complement(2503..4614)
                     /locus_tag="VGB85_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134217.1"
                     /translation="MPQTSARPWSSLLRRSVSIHGAGWARGFLLGLVVAACGRSDYAI
                     PCGSDEDCGEGGVCTPEGQCVIFDEECRLDSDCASGEKCLDFQCVAPQVCDDDLECPP
                     GTLCVREDGDDFGVCQGDTPPLCPACPAGTTCDGKGGCIPDPPGPCEFDFECPEGQVC
                     IDGACTASQACFEDFECPPGQSCIDGQCVQTLCEFDFECDFGEVCIGGVCVPVGDMCQ
                     FDSDCPFDQVCQNGQCIFPECFSDFDCPSGFDCIDNFCQFNPQCIDDIDCPPGNACVD
                     FECVPQPECLSDFDCEPGQLCLGGFCVDQPPMCASDLDCLPGQTCQNGMCVDPPPMCT
                     LDTDCLPGQTCQNGVCVDPPPVCMTDADCQPGETCQGGVCLPPPPQCQTDADCGPDQL
                     CFMNQCVPLPPECVVDEHCDVVNGETCVGGVCTPPECTLDSQCDADELCFQFKCVPLP
                     PECVVDEQCDANETCQGGVCVPDPACVDDADCQLGQICVNNLCVAPPPCVDDNDCPPG
                     QACIANACVVVPDCLFDTDCQPGEKCENGTCVPGGCTLDTDCQPGELCQGGVCVVPPE
                     CTLDTDCQPDESCQAGQCVPLPECAVNADCKSGFVCQAQTCVFAGECLTDEHCAPGQL
                     CQAGACIGDPECVDDLSCPPGEACVFGDCVAPALCKTNKDCKGAGEVCVGGFCSGGVN
                     CNDNTDCDAGQACFENQCVLI"
     gene            4955..>5150
                     /locus_tag="VGB85_09060"
     CDS             4955..>5150
                     /locus_tag="VGB85_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134218.1"
                     /translation="MTRHHTLRGHHTLQRLIGAWAAIATLYVAGPATAAPAKPATAVP
                     VAAPAAGQPAARPAAAKPAAP"
BASE COUNT          784 a         1824 c         1845 g          697 t
ORIGIN      
        1 tgagccattc cgcgagcaac gcctcgagca gttgaaggcg ctggccgctg gccgcggcgg
       61 agcgtgcctc gacgaggtct acttccgcaa aaggcacccg atgcgctggc gctgccagca
      121 cggacacgag tgggaggcac agagcgacag cgtcctgcgc ggcaactggt gcccgttctg
      181 tagcggccga cggaccaccg tcgcggccct ccaggcgctc gccgcggagc gcggcggcga
      241 gttcctgtcg accgaagtcg gccgcaagat cgatccacac cgctggcgct gcgctcatgg
      301 gcacgagtgg cccgccgcac cgtcgaagat catcgagggg acgtggtgcc cgacctgcgc
      361 cgggcgcaac acgatcaccc tggccaccct gcaggagctg gcgcgcagtc gcggcggagc
      421 ctgcctcgcc cagcaataca ccagcatcaa ccagcatgtc cgctggcgct gcgacgccgg
      481 gcacgaatgg agcgcgaccg ccaactccac caggcgcggg acctggtgcc cgacctgcgc
      541 cgggaactct cgcggcagcc tgtcgcgcct gcgagaggtc gcgcgcgagc gcggcgggaa
      601 gtgcctggcc tctgcctaca tcaactcgcg aacccccgtc ccatggcgct gcgccgaggg
      661 ccacacctgg tcggccgcgc cgagcaacgt cgtcgccggc ggatggtgtc gggagtgttc
      721 gtaccacatc cgaagatcga agcggcccac gctcgccgac ctcgtcgcgc ggtgccgcga
      781 acaggccggg cgggtcctgc tcgacctccc ggacgacacc gtcatccacg gagatacacg
      841 ggcgaccttt cagtgcgtca tgggccatac ctggacgacg gcagtgcaca ccgtccgttc
      901 gggtcactgg tgtcccgagt gcgcgggcgt ggcccgcggc ggcctcgcgg cgctgcaggc
      961 catcgccgcc gagcgcggcg gtcgctgcct cgccaccgag tacatcgaca accgcacgcc
     1021 gatcccgttc gagtgcgccg agggccaccg ctggaacgcc cggcccttcg ggatcaagcg
     1081 gacctggtgc ccgcagtgcg cccccacccg ccggctcgac ctcaccatgt tgcgcgccga
     1141 cgccagcgcc cgcggcggcc gttgcctcgc cgacgtcctg cccggccgcg accaaccgat
     1201 cgcgtgggcc tgcgcgcagg gccaccagtg gatggcgcga ccgaccgacg tccacggcgg
     1261 gtcctggtgc ccgaagtgca ggggcgtcgg tacctacacg ctcgagcgcg cccggaaagc
     1321 cgccgccagg cacggtggcg agtgcctgtc gaccgcctgc gcacgcgcca ccgacgtcct
     1381 ctcctggcgc tgccgcgagg gccacacgtg gaccagcgcc gcgatgtatg tcctgcgcat
     1441 cgggacctgg tgcccacgct gcggccgtga gcgcggcgct gcgaagctcc gcgagtcttt
     1501 ttcggccacg gggtgacgtt ccggcgcgcg ggtccctgta gcccctttgg tgtggtggtc
     1561 cccgcgtagt gcggcgcggg tggtgtggcg ggagcaggtc gggcgcgacc tttgcggtcg
     1621 agagcatctg tggcgagcac gccgcgctcc actgcttcga gcagccatga cgcgaagcag
     1681 ccgccgcggt cgcactccga gcagcaggcc aggtggtccg tgtggcgccg gaccgacgtc
     1741 ggggagcagt gcctgcgcga cgagaaatgc ccgggccccg gtcgcggttt cagtgccggg
     1801 gacgccgact cgaaggtctg cgtgcccgcg tctgcgcaga ttcgcgccgc gcagaatcgc
     1861 tcacacgtgt cggcgacggc cccgaacgcg agcccgggag gccacgtggg gccctcaaag
     1921 gcctcctggg aggtgatcag ggccagaaat tcgcaatgat ctgcgcgaga caaggcgtgc
     1981 ctagtacaag gcattgcagc catgaagccg accgtgccgc cgatcaccct cgaagacttc
     2041 aacctcgaag ccacggagcg ccggctctgc gtcgaggccc tccggctcgc cggcaatatc
     2101 gtcggtgcgg ccgagatcct cggcatcacc cggcacgcgc tcaagcgtcg catcatcaag
     2161 ctcgacatcc cgtggtcgcg caacggatcg atcgaccccg acacgtccac gtagcgccgg
     2221 acctccggct actcgagctc gtcgagcagc gcgtcgaggc gtgggtcgcc gaggccgtag
     2281 atctgctggc ctgcgagcgc ggcgaagtcg atggtcgcct cgacctgcca gtcgagcgca
     2341 tccgccaggc cgtgctctac gttcgcgaga tggacacgga ggcggggctc ggcctcgcgc
     2401 tgcaccacga gctgccccag gtggaggcga tcgtgccgca ttcgcggttt tactggacgg
     2461 tcgcgccctg gcagccgaga aagctgccag ggcgaggctg actcagatca gcacgcactg
     2521 gttctcgaag caggcctggc cggcgtcgca gtcggtgttg tcgttgcagt tgacgccgcc
     2581 ggagcagaag ccgccgacgc acacctcgcc ggcgcccttg cagtccttgt tggtcttgca
     2641 cagcgccggt gcgacgcagt cgccgaacac gcaggcctcg ccgggcgggc agctcaggtc
     2701 gtcgacgcac tcggggtcgc cgatgcaggc gccggcctgg cacagctggc cgggggcgca
     2761 gtgctcgtcg gtcaggcact cgcccgcgaa cacgcaggtc tgggcctggc acacgaagcc
     2821 ggacttgcag tcggcattga cggcgcactc gggcagcggg acgcactggc cggcctggca
     2881 cgactcgtcc ggctgacagt cggtgtcgag ggtgcactct ggcggcacga cgcacacgcc
     2941 gccctggcac agctcgccgg gctggcagtc ggtgtcgagg gtgcagccgc cgggcacgca
     3001 ggtgccgttt tcgcacttct cgccgggctg gcagtcggtg tcgaacaggc agtccggcac
     3061 gacgacgcag gcgttggcga tgcaggcctg accgggcggg cagtcgttgt cgtcgacgca
     3121 cggcggcggg gcgacgcaga ggttgttgac gcagatctgg ccgagctggc aatccgcgtc
     3181 gtcgacgcac gcggggtcgg gcacgcacac gccgccctgg caggtctcgt tggcgtcgca
     3241 ctgctcgtcg acgacgcact cgggcggcag cggcacgcac ttgaactgga agcacagctc
     3301 gtcggcgtcg cactggctgt cgagggtgca ctcgggcggg gtgcacacgc caccgacgca
     3361 ggtctcgccg ttgacgacgt cgcagtgctc gtcgacgacg cactcgggcg gcagcggcac
     3421 gcactggttc atgaagcaca gctggtcggg cccgcagtcg gcgtcggtct ggcactgcgg
     3481 cggcggcggc aggcagacgc cgccctggca ggtctcaccg ggctggcagt cggcgtcggt
     3541 catgcacacc ggcggcgggt cgacgcacac gccgttttgg caggtctgac cgggcaggca
     3601 gtcggtgtcg agcgtgcaca tcggcggcgg gtcgacgcac atgccgtttt ggcaggtctg
     3661 gccgggcagg cagtcgaggt ccgaggcgca catcggcggc tggtcgacgc agaagccgcc
     3721 gaggcacagc tggccgggct cacagtcgaa gtccgagagg cactccggct gcggcacgca
     3781 ctcgaagtcg acgcacgcgt tgccgggcgg gcagtcgatg tcgtcgatgc actgcgggtt
     3841 gaactgacag aagttgtcga tgcagtcgaa gccggacggg cagtcgaagt cgctaaagca
     3901 ctccgggaag atgcactggc cgttttggca cacctggtcg aagggacagt cggagtcgaa
     3961 ctggcacatg tcgccgaccg gcacgcacac gccgccgatg cagacctcgc cgaagtcgca
     4021 ctcgaagtcg aactcgcaca gcgtctgcac gcactggccg tcgatgcagc tctggccggg
     4081 cgggcactcg aagtcctcga agcaggcctg cgaggccgtg cacgcgccgt cgatgcacac
     4141 ctgtccctcg ggacactcga agtcgaactc gcagggcccg ggcgggtcgg ggatgcagcc
     4201 gcccttgccg tcgcaggtgg tgcccgcggg gcaggcgggg cacagcggcg gcgtgtcgcc
     4261 ctggcacacg ccgaagtcgt cgccgtcctc gcgcacgcac agggtgccgg gcgggcactc
     4321 gaggtcgtcg tcacacacct gcggcgcgac gcactggaag tcgaggcact tctcgccgga
     4381 cgcacagtcg ctgtcgaggc ggcactcctc gtcgaagatg acgcactggc cctcgggggt
     4441 gcacacgccc ccctcgccgc aatcctcgtc ggacccgcag gggatcgcgt agtcgctgcg
     4501 cccgcaggcg gcgacgacga gccccagcag gaagccgcgc gcccacccgg ctccgtggat
     4561 cgagacgctt cggcgcagca gcgaggacca gggacgagct gaagtttggg gcatggcggc
     4621 gatccagcga acggggccag gggaggccga aagtcgaagg caggtcagtc aacaagtcag
     4681 gaaagaccga gcgcatcccc cgcggggcgg ctcgaccctc tcactgtaga gccgcctgcg
     4741 cgccgcgagc aagcgaatgg acatccggac cctgcgcaca cccgcgccgc ccgggaccgc
     4801 cgcagtttgc tggccacggc cgccgacgtg cggcaagctg gggcccccgg tgtatggccc
     4861 caggagattc ccgacgcgtt cccgttctcg cgcgtcgcgt gaacctgcgg gcccgccgga
     4921 tcccggggcc cacggcggcc cggcgacccc tgccgtgacg cgccaccaca ccctgcgggg
     4981 ccaccacacc ctgcagcgcc tgatcggagc gtgggcggcg atcgctacgc tgtacgtcgc
     5041 cggtccggcg accgccgccc cggcgaagcc ggcgaccgcg gtgccggtcg cggctccagc
     5101 tgcggggcaa cctgcggcca ggccggcggc tgcgaaaccg gccgcgccgg
//
LOCUS       DASWNJ010000536         4161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_503805, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000536 DASWNJ010000000
VERSION     DASWNJ010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503805"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..429
                     /locus_tag="VGB85_09065"
     CDS             148..429
                     /locus_tag="VGB85_09065"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0134219.1"
                     /translation="MTARTRRRHSAEFQANAVRKLSEPGQSAQELADELEVRPSLLYR
                     WRRAAQAKEGPMPKKKPPPTNEPSRRPQDWTTEQKLAVVLGALHFHASS"
     gene            complement(522..1073)
                     /locus_tag="VGB85_09070"
     CDS             complement(522..1073)
                     /locus_tag="VGB85_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134220.1"
                     /translation="MTLWPDFRVVKDLVFFAWAAPDEASYEPPANRTETESFQNHTHV
                     LDLFTHGASLGEEPFWDAAHPDFAAACRFGKRWAHAVAVKLWFEFPGRAFFVYYTEKD
                     NPIVRFHQQHAGEQPWLAPEGSEERIARGEVVIWEVRSRDPAFLPVRPVDGQAASAVP
                     APRSTRAAAPAGRPRGRQTRPQQ"
     gene            1279..1662
                     /locus_tag="VGB85_09075"
     CDS             1279..1662
                     /locus_tag="VGB85_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134221.1"
                     /translation="MTTVLYLGLDPRVVDFSLLPDLTEEKLTAQLDEQVAQLRAAGFD
                     AVFVGVDRGETAAAVTASALASRPHDIVLIGAGLRASPLLLELFENLINVVHERAPRA
                     RICFNTTPRDSEAAVRRWADAARGG"
     gene            complement(1788..2213)
                     /locus_tag="VGB85_09080"
     CDS             complement(1788..2213)
                     /locus_tag="VGB85_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134222.1"
                     /translation="MTEIWNTLRYRGPHGRNGRVELTLWSDHRARLNSFVAQPVTPAV
                     VQGVLTNWVVPELFENLHAAGFPDLPKNNAGRVALLSVAGKAGMVQAWLAPTHGANSP
                     MARAVYLLEGVAHALSGGTLSSFRTLERPVLAEALPGPP"
     gene            2766..3830
                     /locus_tag="VGB85_09085"
     CDS             2766..3830
                     /locus_tag="VGB85_09085"
                     /inference="COORDINATES: protein motif:HMM:NF039450.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADYC domain-containing protein"
                     /protein_id="HEY0134223.1"
                     /translation="MPLETRTIVLLALCAAVLVGACDDVDSKIQPGPSELVTERALVG
                     NDLLLNSASLNGWSLNGFRTNGWSLNGWSLNGWSLNGWSLNNVSLSGSAFTGVASIDG
                     EQVVLVGEDLIGTELALSHEGVPFTLRFDDIYINPADPDGDVYFYKVTVHDPDTDTWT
                     SLCRDSYGEPIEAIALKHHWNPNTGARVDDPVAVTLACRGAALAKCVEWGYRPWATVE
                     SCDDGVCTQVNLTDHHQACTRMARADYCGDGVPHTLNNTPIDIYDRLKPKIQTQSSSG
                     KDWKVEAEWGPDGAQCLGKQLRVDMFAALNIAHTAPPCRAALTAVKNCGQFLGNRPNS
                     KLGNHYCAYWNTDPAKCALP"
     gene            complement(3908..>4161)
                     /locus_tag="VGB85_09090"
     CDS             complement(3908..>4161)
                     /locus_tag="VGB85_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134224.1"
                     /translation="LKAMVADYTVPAQRATAFGVFHGSIGLAALPASLLFGIFWARLG
                     PQAAFSIGAGLAALAALGLLMLSPQHPGPVPLGVRDKAS"
BASE COUNT          740 a         1436 c         1361 g          624 t
ORIGIN      
        1 cgacgaagcg atcgagccgg catcggcgag tccggcgaag ccggcgacgg cggtccttgt
       61 gatcatcccc agagggacca gattcaaggt cgacatggag agccaccctg gcgcgacctg
      121 cctgaggatc ccggtaggag aggcgacgtg acggcgagga cccgccggcg gcactcggcc
      181 gagttccagg cgaatgcggt gcgcaagctg agcgagccag gacaatcggc gcaggaactt
      241 gccgacgagc tcgaggtacg tccgtccctg ttataccggt ggcggcgcgc agcgcaggcc
      301 aaggaaggtc caatgccgaa gaagaagccc ccaccgacga acgaacccag ccgcaggccg
      361 caggactgga cgaccgagca aaagctggcg gtggtgctcg gtgctctcca tttccacgct
      421 tcatcctaac atccgccacg cgcaccggcg ggcggtgcct ggcctcgcgc cgcacagagc
      481 aggcaccgat gtgctggttc gcaccgccgg tatgtcctgc atcactgctg cggacgcgtt
      541 tgccgacctc gcggacgacc cgccggggcc gcggccctcg tggatcgagg cgctggcacg
      601 gcgctcgccg cctgcccgtc caccggccgc accggtagga acgccgggtc gcggctgcgg
      661 acctcccaga tcacgacctc gccgcgggcg atccgctcct ccgagccctc cggagcgagc
      721 cacggctgct cccccgcgtg ctgctgatgg aagcggacga tcgggttatc cttctcggtg
      781 tagtagacaa agaaagccct gcccgggaac tcgaaccaca gcttcaccgc cactgcatgc
      841 gcccagcgct tgccgaagcg acacgcagcc gcgaagtctg ggtgcgcggc gtcccagaac
      901 ggctcctcgc cgaggctcgc gccgtgggtg aacagatcga ggacgtgcgt gtggttttgg
      961 aagctctccg tctccgtgcg gttcgcgggc ggttcatagc tcgcctcgtc cggcgcggcc
     1021 caggcgaaga acacgaggtc cttgacgacc cggaagtctg gccagagggt catagccgtc
     1081 ttcgcggccc gatcaacggc gacgttttct acgttcagct cgtcggcaga tccttcggcg
     1141 tcctcggaca tccgcggcat ggtaccttcg atgatcgaac ccgaggccgg gagtcctaac
     1201 ggcagtcaca agtcatggtc caacgcgtgg tgtcggtcga ggcaccccga cgagcgccgg
     1261 tggtaccatc gcccggccat gacaaccgtc ctgtacctgg gtctcgaccc gcgcgtcgtc
     1321 gacttctccc tgctgcccga cctgaccgag gagaagctca ccgcccagct cgatgagcag
     1381 gtcgcgcaac tgcgtgcggc cggcttcgac gcggtcttcg tcggcgtcga ccggggcgag
     1441 accgccgccg ccgtgacggc aagcgcgctc gcgtcgaggc cccacgacat cgtcctgatc
     1501 ggcgcgggcc ttcgcgcctc gccgctgctg ctggagctgt ttgagaacct tatcaacgta
     1561 gtgcacgagc gtgcgccgcg ggcccgcatc tgcttcaaca ccacaccccg cgacagcgag
     1621 gcggccgtcc gccgctgggc cgacgcggcg agaggtgggt gatcgacgct gctccgccgg
     1681 cacccactcg agcttgaacg agttcattgg tgcatgagag gcgcctgccc gtgtagcaag
     1741 ctcatccagc actcgccgtt atcggcggcc agtccatcgt tgcggtgtta cggcggtccc
     1801 ggcaatgctt ccgccagcac gggtcgctcc agggtacgaa aggacgacaa cgttccaccg
     1861 ctaagggcat gggcgacacc ttcgaggagg tacaccgcgc gggccatcgg cgagtttgcc
     1921 ccgtgtgtcg gagcaagcca cgcctgaacc atcccggcct tgccggcgac ggacagcagt
     1981 gccacacggc cggcgttgtt cttcggtagg tccggaaacc cggcggcgtg aaggttctcg
     2041 aacaactccg ggacgaccca gttcgtcagg acgccctgga caactgccgg agtgaccggc
     2101 tgcgcgacaa aagaattcaa gcgggcgcga tggtcactcc acagcgtcag ttcaacccgc
     2161 ccgttgcgcc catgaggacc gcggtagcgc aaggtgttcc agatctcagt catcgagggc
     2221 gaacgtaaaa ctacgtcagg aatgtgtccg gcgtgcccga cgctggtcgc cagtccacgg
     2281 agtcactcga gaggaccgat cgagcgttcg ccgtgttcac tgcgcggtat ccgcggtccc
     2341 cagtcgtgga ggggtgcccg cggctcaatt gccggggttc aaacgagcct ggatgcggtt
     2401 tcagcttgtc gtagatatcg atgggcgtat cgtcggaggc ggaggccgag acggcccgag
     2461 cggggccatg tgcgtcgccg ggaacggcgc agtccgcccg cgcaagtgct gccacgcctc
     2521 ccgagcggct cgctggacgg tccgggcgat gaaatgagcg cagccgcgaa gctgccattg
     2581 ttcaccactc aatccgacat acgtgtacga ccaacgaagg atcccgacgt aacccgcatg
     2641 agcttcgtct gaaacagcag ggtcggcgca agtccgccgc ggctttgctg cttacgacct
     2701 tttcgcagcg cttgcatccg cccccgacgg gcgcaaaaaa cgggcgagac cgcgaggtga
     2761 tgcatatgcc gctcgaaaca cgaaccatag ttctgctcgc cctgtgcgcc gcggtgctcg
     2821 tcggcgcctg tgacgacgtc gactccaaga tccaacccgg cccctctgag ctggtcacgg
     2881 agcgcgcgct cgtcggcaac gacctcctgc tcaactcggc cagcctcaac gggtggagcc
     2941 tcaacggctt tcgcaccaac gggtggagcc tcaacgggtg gagcctcaac gggtggagtc
     3001 tcaacgggtg gagcctcaac aacgtctcgc tgtccggcag cgccttcacc ggcgtggcgt
     3061 cgatcgacgg cgagcaggtc gtgctcgtcg gcgaggatct gatcggcacc gagctggcgc
     3121 tgtcccacga aggcgtgccg ttcacgctgc gcttcgacga catctacatc aacccggccg
     3181 accccgacgg cgacgtgtat ttttataagg tcacggtgca cgaccccgac accgacacct
     3241 ggacgagcct gtgtcgcgac agctacggcg agccgatcga ggcgatcgcg ctcaagcacc
     3301 actggaaccc gaacaccggc gcgcgcgtcg acgaccccgt cgcggtcacg ctcgcgtgtc
     3361 gcggcgccgc gcttgcgaag tgcgtcgagt ggggctaccg cccgtgggcg accgtcgaga
     3421 gctgcgacga cggcgtctgc acgcaggtca acctgaccga ccaccaccag gcgtgtactc
     3481 gcatggcccg cgccgactat tgcggcgacg gcgtgccgca cacgctcaac aacaccccga
     3541 tcgacatcta cgaccgcctg aagccgaaga tccagaccca gtcgagctcc ggcaaggact
     3601 ggaaagtcga ggccgagtgg gggcccgacg gagcacagtg cctcggcaag cagctgcggg
     3661 tcgacatgtt cgccgccctc aacatcgccc acaccgcgcc gccgtgccgc gccgccctga
     3721 ccgccgtcaa aaactgcggc cagttcctcg gcaaccgccc caacagcaag ctcggcaacc
     3781 actattgcgc gtactggaac accgacccgg ccaagtgcgc gctgccctga gcgcccgcga
     3841 tgggtggctg tcgccgaccg ctcgaggagg ccgcccacgt actcgccggt gccggaggtt
     3901 cggcacttca agaagccttg tcccggacgc cgagaggcac gggccccggg tgctggggag
     3961 acagcatgag cagcccgagc gctgccagag ccgcgagccc ggccccgatc gaaaatgcag
     4021 cctgcgggcc gaggcgcgcc cagaagattc cgaagagcag gctcgcgggc agagctgcca
     4081 acccgatcga gccgtggaat acaccgaagg cggtcgctcg ctgagcgggc acggtgtagt
     4141 cggcgaccat cgccttgagc g
//
LOCUS       DASWNJ010000537         2450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_292729, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000537 DASWNJ010000000
VERSION     DASWNJ010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292729"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VGB85_09095"
     CDS             <1..565
                     /locus_tag="VGB85_09095"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosine deaminase family protein"
                     /protein_id="HEY0134225.1"
                     /translation="TLHSAVPFRPWIGGVGLDSSEAGNPPSKFQEVFDEAREANFPVV
                     AHAGEEGPASYIIEALDLLGARRIDHGVRCVEDEALVERLVRDQIPLTVCPLSNIKLR
                     VFKAMGEHNLKQLMDRGLLITVNSDDPAYFGGYVGDNYLAAATALGLTRAQIVALARN
                     SFNASFLGEERKQALLGEVERFAGSAA"
     gene            817..1593
                     /locus_tag="VGB85_09100"
     CDS             817..1593
                     /locus_tag="VGB85_09100"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEY0134226.1"
                     /translation="MNTISKIFLPIVLLFACDKEDTDNAEPNDAAVESNDGDRGHRGR
                     GGLKKLDTDGDGMISQAEARDSRLADKFAELDADKDGKLSKDELHAMKGKGHDPAERA
                     AHMLKKFDANNDGAITQAEVGDHPKLADKFAELDADKDGKLTSTELQAMRGPGHGGGH
                     GKGHDKGEWHDMDPAQRAAKMMEKFDADKDGSLSAAELAEHPKLAGKFAEVDTDKDGK
                     LSSAELTAFKAAHHGKGRGGPDGERGRGRGRGPDGDKSHG"
     gene            complement(1757..>2450)
                     /locus_tag="VGB85_09105"
     CDS             complement(1757..>2450)
                     /locus_tag="VGB85_09105"
                     /inference="COORDINATES: protein motif:HMM:NF023459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfbK domain-containing protein"
                     /protein_id="HEY0134227.1"
                     /translation="DHTMEQLADKGNGNYAYIDSEREAKKVLVDEAAGTLVTIAKDVK
                     MQVEWNPNAVAAYRLIGYENRMLAAQDFADDRKDAGELGAGHSVTGIYEVVPAGTPAP
                     GGKVAPLKYQRAAAGSTAELLTVKLRYKQPEGQSSVEFALAVANEAAKGGTSDNFRMA
                     AAVAAFGQRLRDPDSKDGLTFAQIHELAATVELPDPHGHRKEFLELVRRAGQLAAPTK
                     KPVPPLQDDELG"
BASE COUNT          392 a          814 c          894 g          350 t
ORIGIN      
        1 tacgttgcac agcgcggtgc cgtttcgccc gtggatcggc ggcgtcggcc tcgacagctc
       61 ggaggcgggc aaccctccga gcaagttcca ggaggtgttc gacgaggccc gcgaggccaa
      121 cttcccggtc gtcgcgcacg cgggcgagga gggcccggcg tcgtacatca tcgaggcgct
      181 cgacctgctc ggcgcgcggc gcatcgacca cggggtgcgc tgcgtcgagg acgaggcgct
      241 ggtcgagcgt ctggtgcgcg accagatccc gctgacggtg tgcccgctgt cgaacatcaa
      301 gctgcgcgtg ttcaaggcca tgggcgagca caacctcaag cagctgatgg accgcggcct
      361 gttgatcacg gtcaacagcg acgacccggc gtacttcggc ggctacgtcg gcgacaacta
      421 cctcgcggcc gcgaccgcgc tcggcctgac gcgcgcgcag atcgtcgcgc tggccaggaa
      481 cagcttcaac gcgtcgttcc tcggcgagga gcgcaagcag gcgttgctcg gcgaggtcga
      541 gcgcttcgcc ggcagcgcgg cctgaggcgg gcctgggacc ggcgcggacg ccgtggtccg
      601 cggatgcgcg cgcagtgttg ccggcgggta aagccaggct tcacgagacg ccgcgcaggg
      661 gcgcgtggcg ggcgtttggc gggcgtttgg cgtcgggagg tccttggcga gcggtgcggg
      721 ctgtaaagac cccgtaaacg ggcgggcgcg gggggctggg tggccgaata acaggagcat
      781 cgccggggtc gatagccccg caagagagaa cccgccatga acaccatcag caagatcttc
      841 ctccccatcg tcctcctgtt cgcctgcgac aaggaagaca ccgacaacgc cgagcccaac
      901 gacgccgccg tcgagtcgaa tgacggagac cggggtcacc gcggtcgcgg cgggctcaag
      961 aagctcgaca ccgacggcga cggcatgatc tcgcaggccg aggccaggga ctcgcggctc
     1021 gccgacaagt tcgccgagct cgacgccgac aaggacggca agctcagcaa ggacgagctg
     1081 cacgcgatga agggcaaggg ccatgacccg gccgagcgcg cggcgcacat gctcaagaag
     1141 ttcgacgcca acaacgacgg cgcgatcacc caggccgagg tcggcgacca ccccaagctc
     1201 gccgacaagt tcgccgagct cgacgccgac aaggacggca agctgacctc caccgagctc
     1261 caggccatgc gtggccctgg ccacggcggc ggccacggca agggccacga caagggcgag
     1321 tggcacgata tggaccccgc ccagcgcgcc gcgaagatga tggagaagtt cgacgccgac
     1381 aaggacggct cgctctccgc cgccgagctc gccgagcacc cgaagctcgc cggcaagttc
     1441 gccgaggtcg acaccgacaa ggacggcaag ctcagctccg ccgagctgac cgccttcaag
     1501 gcggcccacc acggcaaggg ccgcggcggc ccggacggcg agcgcggaag gggccggggc
     1561 cggggccccg acggcgacaa gagccacggc tgaggctctc gctgcgtgaa gagaggcgag
     1621 cgcgggcacc tgcccgcgct cgtcgtttgt cgtgacgaag gcgaacgagg cggtgcctcg
     1681 ctcgcctgct ttgtgacaca tgagcacggc gagcgaggca ttcctcgctc gccgtatttc
     1741 tattttttaa ttaaactcag ccgagctcgt cgtcttgcag cgggggcacc ggcttcttcg
     1801 tgggcgcggc gagctggccg gcgcggcgca cgagctcgag gaactccttg cggtggccgt
     1861 gcggatcggg cagctcgacg gtggcggcga gctcgtggat ctgggcgaag gtcaggccgt
     1921 ccttgctgtc ggggtcgcgc aggcgctggc cgaaggcggc gacggccgcg gccatgcgaa
     1981 agttgtccga ggtcccgccc ttggcggcct cgttggcgac cgcgagcgcg aattcgacgc
     2041 tgctttgccc ctcgggctgc ttgtagcgca gcttcacggt gagcagctcg gcggtcgatc
     2101 cggcggcggc gcgctggtac ttgagcggcg cgaccttgcc accgggggcg ggcgtgccgg
     2161 cgggcacgac ctcgtagatg ccggtgacgc tgtggccggc gccgagctcc ccggcgtcct
     2221 tgcggtcgtc ggcgaagtcc tgcgcggcga gcatgcggtt ctcgtagccg atcaggcgat
     2281 aggccgcgac cgcgttcggg ttccactcga cctgcatctt gacgtccttg gcgatcgtga
     2341 cgagcgtgcc ggcggcctcg tcgacgagca cctttttggc ctcgcgctcg gagtcgatgt
     2401 acgcgtagtt gccgttgccc ttgtcggcca gctgctccat cgtgtggtcg
//
LOCUS       DASWNJ010000538         1772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_150950, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000538 DASWNJ010000000
VERSION     DASWNJ010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150950"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1772
                     /locus_tag="VGB85_09110"
     CDS             <1..>1772
                     /locus_tag="VGB85_09110"
                     /inference="COORDINATES: protein motif:HMM:NF024574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0134228.1"
                     /translation="EAIAAWREVLDKHGDVDIALEALQNLYEKENRWADAAEILRRKI
                     QQATDENVAIDLLGRLADMHADRLQQPAEAVLVWAEVARRRPGHERANNALRTAYVAE
                     GRWDDLVALYEAQGRLGGVIDVLYAAADGLTDEAERLSLYRRIAALARDRLADAERGL
                     QALEKMLAIQPDNQQVARQLLPIYRERGDWTRVLAIYEVLLAGSADDDDRLDTIAVMR
                     DVALQKLNDPRQAMHWAGQAYKLRPGDQVLRAGLESVAEKADGWDELARYYEARVQSP
                     ECDTGERLELLDKLATIARERLGAPEEAQRYLRKIIELEPTNRPAMASLEEMYTATEQ
                     WKNLAEIYDIRLTVTTDPGARVAVLRRLGVLQEQRIGDLDAAIDTYRQIQSAAPSDAE
                     TIESLTRLYRARERWPELAEILQRRLDGVELADQHIPMLFELSHIYASRLRQSSKAIT
                     GFLQILELDPQHLPTIDALEVLRRSDPGSAVAIMRGLLPYYRSIQDHGREAEAMEVLV
                     MAEPDSPARTSQLVSLANLYAQMSGRAEDALRVRRELLIAQPDDGLLRSEVERAAREL
                     GRMDLVASAYEQILKNLQRKADEA"
BASE COUNT          291 a          656 c          611 g          214 t
ORIGIN      
        1 cgaggcgatc gcggcgtggc gcgaggtgct cgacaagcac ggcgacgtcg acatcgccct
       61 cgaggcgctg cagaacctgt acgagaagga gaaccgctgg gccgacgccg cggagatcct
      121 gcgccgcaag atccagcagg ccaccgacga gaacgtcgcg atcgacctgc tcggtcgcct
      181 cgccgacatg cacgccgacc gcctgcagca gccggcggag gccgtgctcg tgtgggccga
      241 ggtcgcgcgc cgcaggcccg ggcacgagcg ggccaacaac gcgctgcgca ccgcctacgt
      301 cgccgagggc cgctgggacg acctcgtcgc gttgtacgag gcgcagggcc gcctcggcgg
      361 cgtgatcgac gtgctctacg cggccgccga cggcttgacc gacgaggccg agcgcctgtc
      421 gctgtaccgt cggatcgccg cgctcgcgcg cgaccgcctc gccgacgccg agcgcggcct
      481 gcaggcgctc gagaagatgc tggcgatcca gcccgacaat caacaggtcg cccgccagct
      541 gctgccgatc taccgcgagc gcggcgactg gacccgcgtg cttgcgatct acgaggtgct
      601 gctcgccggc tcggccgacg acgacgaccg cctcgacacg atcgccgtca tgcgggacgt
      661 cgcgctgcaa aagctcaacg accccaggca ggcgatgcac tgggctgggc aggcgtacaa
      721 gctgcgtccg ggcgaccagg tgctgcgcgc cggcctcgag tcggtcgcgg agaaggccga
      781 cggctgggac gagctcgcgc gctactacga ggcgagggta cagagccccg agtgcgacac
      841 cggcgagcgc ctcgagctgc tcgacaaatt ggcgacgatc gcccgcgagc gcctcggcgc
      901 gcccgaggag gcccagcgct acctgcgaaa gatcatcgag ctcgagccga ccaaccgccc
      961 ggcgatggcc tcgctcgagg agatgtacac ggcgaccgag cagtggaaaa acctcgccga
     1021 gatctatgac atccgcctga cggtcacgac cgaccccggc gcgcgcgtgg ccgtgctgcg
     1081 ccgcctcggc gtgttgcagg agcagcgcat cggcgacctc gacgcggcga tcgacaccta
     1141 ccgccagatc cagagcgccg cgccgagcga cgccgagacg atcgagagcc tgacccgcct
     1201 gtaccgcgcc cgcgagcgct ggcccgagct cgccgagatc ctgcagcgcc ggctcgacgg
     1261 cgtcgagctc gccgaccagc acatcccgat gctgttcgag ctgtcgcaca tctacgcctc
     1321 gcgcctgcgc cagtcgtcga aggcgatcac cgggttcttg cagatcctcg agctcgaccc
     1381 gcagcacctg ccgacgatcg acgcgctcga ggtcctgcgt cgcagtgacc ccggcagcgc
     1441 ggtggcgatc atgcgcggcc tgttgccgta ctaccgcagc atccaggacc acggccgcga
     1501 ggccgaggcg atggaggtgc tggtgatggc cgagcccgac tcgccggccc gcacctcgca
     1561 gctggtcagc ctcgccaacc tctacgcgca gatgtccggc cgcgccgagg acgccctgcg
     1621 cgtccgtcgc gagctgctga tcgcccagcc ggacgacggc ctgctgcgca gcgaggtcga
     1681 gcgcgccgcg cgtgagctcg gccgcatgga cctcgtcgcc agcgcctacg agcagatcct
     1741 caagaacctc cagcgcaagg ccgacgaggc cg
//
LOCUS       DASWNJ010000539         1516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_468732, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000539 DASWNJ010000000
VERSION     DASWNJ010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468732"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /gene="rpmF"
                     /locus_tag="VGB85_09115"
     CDS             <1..368
                     /gene="rpmF"
                     /locus_tag="VGB85_09115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEY0134229.1"
                     /translation="LIWADAPRRPTRARRLTAVRPWLISRRPKTTGRMSLARARRATW
                     LAPPLPTRTDPVAVPKKKTSKSRRDMRRSHHALVAPAVVEDKQSGEFRRPHHVDLKTG
                     MYRGRQVIVKREQADAGEE"
     gene            complement(454..528)
                     /locus_tag="VGB85_09120"
     tRNA            complement(454..528)
                     /locus_tag="VGB85_09120"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(494..496),aa:Met,seq:cat)
     gene            complement(566..>1516)
                     /gene="mnmA"
                     /locus_tag="VGB85_09125"
     CDS             complement(566..>1516)
                     /gene="mnmA"
                     /locus_tag="VGB85_09125"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEY0134230.1"
                     /translation="ERLGIAHYVLDFEERFRRAVIDDFAESYARGVTPVPCVRCNQRI
                     KFRDLADIARDLGAVALATGHYAERREGRAGPELHRARDPGKDQSYFLFATTPEQLAY
                     LRFPLGDLTKDETRAHARRLGLDLAEKAESQDICFVPSGHYSDVVAKLRPEAMRPGEI
                     VHTEDGRVLGRHEGVGRFTVGQRRGLGVAARDGEPLYVVAVEAGRRRVVVGPRAAGLT
                     AAASLVEVNWLGDAPPSQAGVTLVAQHRYKSPPVAARLLSGGTEGEAEVRFASPEPGV
                     APGQACVLYAGTRLLGGGWIGGVEPAAAAGASLDRAGAAA"
BASE COUNT          227 a          551 c          539 g          199 t
ORIGIN      
        1 tccttatctg ggcggacgcg ccgcggcggc caacccgggc gaggcgattg accgccgtgc
       61 gcccatggct tatctcccgg cgacccaaaa cgacgggaag gatgtcgctc gcgcgtgcgc
      121 gacgcgcgac gtggctcgcc ccgcccctcc ccacgaggac cgatcctgtg gccgtaccga
      181 agaagaaaac ctcgaagtcg cgccgcgaca tgcgccgctc gcaccacgcg ctggtggcac
      241 cggcggtggt cgaggacaag cagtctggcg agttccgccg cccgcaccac gtcgacctga
      301 agaccgggat gtaccgtggt cgtcaggtga tcgtgaagcg tgagcaggcc gacgccggcg
      361 aggagtaggg cgggcgacgc gcgccgccgc tgtcaagtac ggcgcgcaac ctcgctgctc
      421 agcccgtcga gtgaaccgga acaaagggtc ggatggtagc ggaggaggga tttgaacccc
      481 cgaccaaggg attatgattc ccctgctcta ccactgagct actccgccac gagcccgggc
      541 acgccgcctc gcggtgccgc gtgatctagg cagcggcccc tgccctgtca agcgacgctc
      601 ccgcggccgc ggccggctcg accccgccga tccagccccc gcccagcagg cgcgtgccgg
      661 cgtagaggac gcaggcctga ccgggtgcca cgccgggctc gggcgaagcg aaccggacct
      721 cggcctcgcc ttcggtcccg ccagacagca gccgagccgc gacgggcggg ctcttgtagc
      781 ggtgctgggc caccagcgtg acgccggcct gcgatggcgg ggcgtcaccc agccagttca
      841 cctcgacgag cgacgcggcg gcggtcagcc cggcagcgcg agggccgacg acgacccggc
      901 gccggcccgc ctcgaccgcg acgacgtaga gcggctcgcc gtcacgcgcc gcgacgccca
      961 ggccccgacg ctgcccgacc gtgaagcggc cgacgccctc gtgccggccg agcacgcggc
     1021 cgtcctcggt gtggacgatc tcgcccggcc gcatcgcctc cggccggagc ttcgccacca
     1081 cgtcgctgta gtggccgctc ggcacgaagc agatgtcctg gctctcggcc ttttcggcga
     1141 ggtcgaggcc gaggcggcgg gcgtgcgccc ttgtctcgtc cttggtgaga tcgcccagcg
     1201 ggaagcgcag gtaagccaac tgctccgggg tcgtggcgaa caggaagtag ctctggtcct
     1261 tccccgggtc gcgcgcgcgg tgcagctcag ggcctgccct accctcgcgt cgctcggcgt
     1321 agtggccggt agccagcgcg acggcgccga ggtcgcgggc gatgtctgcc aagtcgcgga
     1381 acttgatccg ctgattgcag cgcacgcagg gtaccggcgt cacgccacgg gcgtaggatt
     1441 ccgcgaagtc gtcgatcacc gcgcgccgga agcgctcctc gaagtcgagg acgtagtggg
     1501 cgatgccgag gcgctc
//
LOCUS       DASWNJ010000540         2012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_468784, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000540 DASWNJ010000000
VERSION     DASWNJ010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468784"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            753..1514
                     /locus_tag="VGB85_09130"
     CDS             753..1514
                     /locus_tag="VGB85_09130"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0134231.1"
                     /translation="MVGRRGVVRARPDAKHFVLVHGAWHGAWCWYKTVTLLQADGHRV
                     TVLDLPSHGTDGTPAAGVTLAAYAARVIAAIDAAGEPVILVGHSMGGIVISTVAEAVP
                     AKISRLVYVSAFLLPDGSSLLDVATRDAGSLATPNLIVKAEEGVVDINRDGITAVFYG
                     HSKPEDINLARPLLKANALAPFATPLVLTPGNYGSVRRFYISCLEDNAITLAAQQSMV
                     TATPCEAVFPMHTDHSPFFSQPRQLHRIFSAIARA"
     gene            complement(1530..>2012)
                     /locus_tag="VGB85_09135"
     CDS             complement(1530..>2012)
                     /locus_tag="VGB85_09135"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEY0134232.1"
                     /translation="GFIATIERASPVLATDNPIYRDQDRVIVDDAATADELYRRLRPA
                     LPERIGPLTLHGLNPRLRMYRYRTGQRFAPHMDHWYMQDERTITLLTVLVYFNDDFEG
                     GETRFHEQVERTVVPRAGSAVIFQHKLRHEGCALRRGSKYAMRSDVLYRGPEPLRLPG
                     "
BASE COUNT          302 a          728 c          668 g          314 t
ORIGIN      
        1 acacccccgc gaactgccgc gcgcccttgt ccgcgtcgcg actgccgacg accacctggg
       61 cgccgcgcgc cgccagctcg cggaccgccg ccttgccgat gttacccgtc gcacccgtga
      121 ccaggaccgt gatcttcgcc atgccagagc cctatcaccg cgaccgtcgg cccaccaggg
      181 cccccgccgc atacgtggca tgcacgcgcg acatacgacc tcaggtcgtc gtgccgagct
      241 cgcacgggtc gcccgggttc gggcacatct cctccgggac cgagtcctcg atgcactgcc
      301 aggtccacga acgccggctc aacctccgcc ctgcatatct cgtggcgccg atatgcgagg
      361 ggttcgaagt gcggtgatac gcggatcgac gccgccgccc gataggcgac gaccgcggtg
      421 agccggtcga acctcgaccg gcgatccctc ggcgccgccg ggcgatcgcg gcgtcgacgc
      481 tcccggcgcg cggacctcgg tcgcacgcgg cgtcgtcacc gctcgtgcga tctgccgatc
      541 acgccgttgc agagtgcgcc agaccaccgt gtgacacgat cgcgctcgcc ctcggcagga
      601 tctgcgcagc gcatttttat gagggggacg gaatcgagcg cgctgcgaga ttgacagcga
      661 gcgaccccga cagggtaaat tcgccgagga ctcgaaccga tgaggttttc cttaacatga
      721 tcgtggcgcg gcaacctcga cgaaggtttt tcatggtcgg caggcgcggg gtggtccgcg
      781 cgcgacccga cgcgaagcac ttcgtgctcg tacacggggc gtggcacggc gcatggtgct
      841 ggtacaagac cgtgaccctc ctgcaggccg atggccatcg cgtgacggtg ctcgacctgc
      901 cgtcgcacgg gaccgacggc acgcccgcgg ccggcgtgac gctcgcggcc tacgcggcgc
      961 gggtgatcgc ggcgatcgac gcggcgggcg agccggtgat cctcgtcggc cacagcatgg
     1021 gtggcatcgt gatctcgacc gtcgccgagg ccgtgccggc gaagatctcg cggctggtct
     1081 acgtgtcggc gttcttgctg ccggacggct cgtcgctgct cgacgtggcg acccgggacg
     1141 ccggctcgct cgccaccccg aacctgatcg tcaaggcgga ggagggcgtc gtcgatatca
     1201 accgcgacgg catcaccgcg gtgttttatg gccacagcaa gccggaggac atcaacctcg
     1261 cgcggccgct gctcaaggcc aacgcgctcg cgccgttcgc cacgccgctg gtcctgaccc
     1321 ccggcaacta cggcagcgtg cggcgcttct acatatcgtg cctcgaggac aacgcgatca
     1381 cgctcgcggc ccagcagtcg atggtcacgg cgaccccctg cgaggccgtc ttcccgatgc
     1441 acaccgatca ttcgccgttc ttctcgcagc cgcgccagct ccaccgcatc ttctccgcga
     1501 tcgcccgcgc ctgagcacgg tcgcccccct cagcccggca gccgcagcgg ctccgggccg
     1561 cggtagagca cgtcggagcg catcgcgtac ttgctgccgc ggcgcagcgc gcagccctcg
     1621 tggcgcagct tgtgttgaaa gatcaccgcc gagccggcgc gcggaacgac cgtccgttcg
     1681 acctgctcgt gaaatcgggt ctcgccgccc tcgaagtcat cattgaaata tacgagcacg
     1741 gtcagcagcg tgatcgtccg ctcgtcctgc atataccagt ggtccatgtg cggcgcgaac
     1801 cgctggccgg tccggtagcg atacatccgc agccgggggt tgagcccgtg cagcgtcagc
     1861 gggccgatgc gctccggcag cgccggccgc aggcgacgat acagctcgtc ggcggtcgcc
     1921 gcgtcgtcga cgatcacgcg gtcctggtcg cgatagatcg ggttgtcggt ggcgagcacc
     1981 gggctggcgc gctcgatcgt ggcgatgaag cc
//
LOCUS       DASWNJ010000541         1992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_575040, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000541 DASWNJ010000000
VERSION     DASWNJ010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575040"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VGB85_09140"
     CDS             <1..668
                     /locus_tag="VGB85_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134233.1"
                     /translation="YGALVARAEPDPRRALGLAALVPAGLIVLALAGQHHEGVLAAIM
                     LALAVTLGGAAGLLAYTNGDGPRARLLRRLAPVGLLPAPGLVGLIGAALVVLGTARFA
                     GTTLGGHAIWLALLAGLATLLSLRALSRRAGPAGPGAKDSSRGPLIGVAALLLPVIAA
                     GVWPARVLGPSHAVARAWVDMTTRGRCERAVRTGDALARAPAEPSEACGQPLRTLEQG
                     GRP"
     gene            901..>1992
                     /locus_tag="VGB85_09145"
     CDS             901..>1992
                     /locus_tag="VGB85_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY0134234.1"
                     /translation="MNESLVALAPLVIVALWAVLTPLAGPALAHAPGWSRGVALGGVA
                     LALLAALSLLAAPDLAVGATLGADVLVVDRLALGLEILVLVALAAIVAVDPPGPGGAA
                     RHGLVLLAGAGAMLCVHAGDVTVLVGGLELACLATATLLWLARDPAGPAPDPRAGFTW
                     LMGQGVAGGLLWLGLGLLYGATGTTRLGELASRVGAVFMRWGASTVQAAVDLLHSSAA
                     LEPDLTIYARDAAVTGAAPAMLLIPGLCLALVGLLARLAVWPLHLGLPAISRRAGASA
                     LATALVLVRLAAAAALLRLFVAVLHAPRMVYAPYGWGTAAALLGGASAVVGAVAAARA
                     SGLRPLLAWAAVAHAGLVVLGMGAAADFYA"
BASE COUNT          146 a          731 c          826 g          289 t
ORIGIN      
        1 tgtacggtgc gctggtcgcc cgcgccgagc ccgacccgcg gcgcgcgctc gggctcgccg
       61 cgctggtgcc cgcggggcta atcgtgctcg cgctcgcggg tcagcaccac gagggcgtgc
      121 tcgcggcgat catgctcgcg ctcgcggtca cgctcggcgg cgcggccggc ctgctcgcat
      181 atacgaacgg agacgggccg cgcgcccggc tgctgcgccg gctcgcgccc gtggggctgc
      241 tgccggcgcc cggcctggtc ggcctgatcg gcgccgcgct ggtggtcctc gggaccgcac
      301 gcttcgccgg cacgaccctc ggcgggcacg cgatctggct ggcgctgctc gccgggctgg
      361 cgacgctgct gtcgttgcgg gccctgtccc gaagggcagg tccggctgga cccggcgcga
      421 aggacagttc gcggggcccg ctgatcggcg tcgcggcgct gttgctgccg gtgatcgccg
      481 cgggggtgtg gccggcgcgc gtgctcggcc cgagccacgc ggtcgcgcgc gcctgggtcg
      541 acatgacgac gcgcggccgc tgcgagcggg ccgtgcggac cggcgacgcg ctggcgcggg
      601 cccccgcgga gccgtcggag gcgtgcgggc agccgctgcg cacgctcgag cagggaggcc
      661 ggccgtgacg gcgatcgacg ggtcgcgcgt cgccctcgcc gcgggtgtcc cgcggtcggc
      721 cgtgcatgcg tcgcgcgtcg tgctgcgcgt cggagctgcg cgcaggtcgg ccatgaacga
      781 gccgctcgtg tcagccgcac ggatgacgcg ggtcggagct gcgaggaggt cggccgcgga
      841 cgagtcgctc gtgtcagccg cacggatgct gcgcgtcgga gctgcgcggg gaggtcggtc
      901 gtgaacgagt cgctcgtcgc cctcgcgccg ctggtgatcg tcgcgctgtg ggccgtgctg
      961 acgccgctgg cggggcctgc cctcgcgcat gcgccgggct ggtcgcgggg ggtcgcgctc
     1021 ggtggggtcg cgctcgcctt gctggccgcc ctctccctgc tcgcggcgcc ggacctcgcg
     1081 gtcggcgcga cgctcggcgc cgacgtgctc gtcgtcgatc gcctggcgct cgggctcgag
     1141 atcctcgtgc tcgtcgcgct cgcggcgatc gtcgccgtcg atccacctgg accgggcggc
     1201 gcggcgcggc acgggctcgt gctgctcgcg ggcgccgggg caatgctgtg cgtgcacgcg
     1261 ggggatgtca ccgtgctcgt cggcggcctc gagctcgctt gcctcgcgac ggcgacgctg
     1321 ctgtggctcg cgcgcgaccc cgccggcccc gccccggatc cgcgcgccgg cttcacctgg
     1381 ctcatgggac agggggtcgc cggcgggctg ttgtggctcg gcctcggcct cctctacggc
     1441 gcgaccggga cgacgcggct cggcgagctg gcgagtcggg tcggcgcggt gttcatgcgc
     1501 tggggcgcga gcacggtgca ggccgcggtc gacctgctgc acagcagcgc ggcgctggag
     1561 ccggacctga cgatctatgc gcgcgacgcc gcggtgaccg gagcggcccc ggcgatgctg
     1621 ctgatccccg gcctgtgcct cgcgctggtc gggctgctcg cgcgtctcgc cgtgtggccg
     1681 ctgcacctcg ggctcccggc gatctcgcgg cgcgccgggg ccagcgcgct ggcgacggcg
     1741 ctcgtgctcg tgcgcctcgc cgcggcggcg gcgctgctgc gcctgttcgt cgccgtcctg
     1801 cacgcgccgc gcatggtcta cgcgccgtac gggtggggca ccgcggcggc gctgctcggg
     1861 ggcgccagcg cggtggtcgg cgccgtcgcg gccgcgcgtg cgtcggggct gcggccgctg
     1921 ctggcgtggg ccgcggtggc gcacgcgggc ctggtggtgc tcggcatggg cgcggcggcc
     1981 gacttctacg cg
//
LOCUS       DASWNJ010000542         3229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_45034, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000542 DASWNJ010000000
VERSION     DASWNJ010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45034"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="VGB85_09150"
     CDS             <1..1275
                     /locus_tag="VGB85_09150"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0134235.1"
                     /translation="GPLPDLALSPALLARVLRERRGVLLHDVLAEPDLADRKSVQMRG
                     ITGAMAAPVEGLIFYVEWGPREALRASEHAYDDEALRLLVCAAQLVSAIGDSARERQQ
                     LGAAARHGPPAVHGEMIGRSAAIQKLQVLLERVAATTSTVLVLGESGTGKELAAGMIH
                     ALSPRAGKPFVAINCAAIAESLLESELFGHEKGAFTGAVAQHDGVFMRAHGGTLFMDE
                     VGELSPGAQARMLRVLENRTLTRVGGTRELTVDVRLIAATHRDLRRQVAEGRFREDLL
                     YRIGVIQTQLPPLRDRRDDIPLLAQHFLRVIGERVGRRVDSIAPEALEVLTRYRWPGN
                     VRELRNVLERALVLGDGPVLEVDDLPPELLHAAPPTPAATDAVVRPLVELEREAIVSA
                     LAATGGNKARAAALLGIDRTTLYRKLKDHAIV"
     gene            1459..2838
                     /locus_tag="VGB85_09155"
     CDS             1459..2838
                     /locus_tag="VGB85_09155"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEY0134236.1"
                     /translation="MRMRAFLLLLATPACIPWEQPGADPSTTSDPVEPGTTGTTGWQV
                     PPRERGNGPVSTSTTTSTASTSTTSGPDPDTTSLTSTTEPAPAVDLAMLQLGEIHPDP
                     DGKDGGPDSPELLEIVHVGDEPLALAGLEIEARSWPKLAAADLGIADLSLEPGQRLTI
                     ARHALPGDLPDPALTIDDAGIRVAFAADSGLRNADGGALLRSGDQLGDLVIYGAAQPA
                     PWDGDGWIDAPAPAPGSGNSLCRVAELDHDDASDWSVCPPSPGLANDPEVSDTTGDMS
                     GAGDSEATGTTGEPLPAEVVIVEVLSNPPGPGSEEKFTEFIEIVNLGPGTVDLATWTI
                     ADALAEQPTGADPLLYADGDGGCAPETCLAAGQRAILVGNLYNGPTGPGLVLVTDDTT
                     LANAGLAVHEPVALRDGEGVLRSTYRAWPDPLVAPDPALSEQALVRASAEAADAAEAW
                     SFAAPTPGL"
     gene            complement(2835..3008)
                     /locus_tag="VGB85_09160"
     CDS             complement(2835..3008)
                     /locus_tag="VGB85_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134237.1"
                     /translation="MSYFRTEFTSFEEFKREALQDLDDLGKEEIELMREVELRDNFHR
                     RPPRRGRPSYDLD"
BASE COUNT          474 a         1230 c         1093 g          432 t
ORIGIN      
        1 ggtccgctgc cggatctggc gctgtcccct gccctgctgg cgcgggtcct gcgagagcgc
       61 cgcggcgtgc tgctgcacga cgtgctcgcc gagcccgacc tcgccgaccg caaatcggtg
      121 cagatgcgcg ggatcaccgg cgcgatggcg gccccggtcg aggggctgat cttctacgtc
      181 gagtggggcc cgcgcgaggc cctgcgggcc agcgagcacg cgtacgacga cgaggcgctg
      241 cgcctgctgg tgtgcgcggc ccagctcgtc agtgcgatcg gcgacagcgc gcgcgagcgc
      301 cagcagctcg gcgccgcggc caggcacgga ccgccggcgg tccacggcga gatgatcggc
      361 cgctcggcgg cgatccagaa gttacaggtg ctgctcgagc gggtcgccgc gaccacctcg
      421 acggtgctcg tgctcggcga gagcgggacc ggcaaggagc tcgcggccgg catgatccac
      481 gccctctcgc cgcgcgcggg caagcccttc gtggcgatca actgcgccgc gatcgcggag
      541 tccttgctcg agagcgagct gttcgggcac gagaagggcg cattcaccgg ggcggtcgcc
      601 cagcacgacg gcgtgttcat gcgggcccac ggcggcaccc tgttcatgga cgaggtcggc
      661 gagctcagcc ccggcgccca ggcgcggatg ctgcgcgtgc tcgagaaccg cacgctgacc
      721 cgggtcggcg ggacccgcga gctgaccgtc gatgtccgcc tgatcgccgc gacgcatcgt
      781 gacctgcgga ggcaggtcgc cgagggccgc ttccgcgagg acctcctcta ccgcatcggc
      841 gtgatccaga cccagctccc gccgctgcgc gaccgccggg acgacattcc tctgctggcg
      901 cagcacttcc tgcgggtgat cggcgagcgc gtcggccggc gcgtcgacag catcgccccc
      961 gaggcgctcg aggtgctcac gcggtaccgc tggccgggca acgtccgcga gctgcgcaac
     1021 gtgctcgagc gggcgctggt cctcggcgac ggcccggtgc tcgaggtcga cgacctgccc
     1081 cccgagctgc tgcacgctgc ccccccgacg cccgcggcga cggacgcggt ggtccgcccc
     1141 ctggtggagc tcgagcgcga ggcgatcgtc tcggcgctgg cggccactgg cggcaacaag
     1201 gcccgcgcgg cggccctcct cggcatcgac cgcacgaccc tgtaccgcaa gctcaaggac
     1261 cacgcgatcg tctgagctgc tgctttcagc tcgcccgata agcgttcgta atcacgtacc
     1321 aatatttgcc cgtgcatttg ttcgcatgac taccggggcg cgtaaccatc gagcagctcg
     1381 ccgaccggcc gcccccggca aacgaagcat cgacgcttgc cgctgcaacg atcgcacgcc
     1441 gaggggcgaa ctcgggcgat gcgaatgcgc gccttcctcc tcctcctcgc cacgccggcc
     1501 tgcatcccct gggagcagcc cggcgccgac ccctccacca cctccgaccc ggtcgagccc
     1561 ggtaccaccg gcaccaccgg ctggcaggtc ccaccccgcg agcggggcaa tggcccggtc
     1621 tcgacctcga ccacgacctc gacggcctcg acctcgacga cctccgggcc cgatcccgat
     1681 accacctcgc tgacctccac cacggagccg gcgcccgcgg tcgacctggc gatgttgcag
     1741 ctcggcgaga tccaccctga cccggatggc aaggatggtg gccccgacag ccccgagctc
     1801 ctcgagatcg tgcacgtcgg cgacgagccg ctggcgctcg ccggtctcga gatcgaggcc
     1861 cggtcctggc cgaagctcgc cgccgccgac ctcggcatcg ccgatctctc gctcgagccc
     1921 ggccagcgcc tgacgatcgc ccgccacgcg cttcccggcg acctgcccga cccggcgctc
     1981 acgatcgacg acgccggcat ccgcgtcgcc ttcgcggccg actccggcct gcgcaacgcc
     2041 gacggcgggg ccctgctgcg ctcgggcgac cagctcggcg acctggtgat ttacggagcc
     2101 gcccagcctg ccccctggga cggcgacggc tggatcgacg cgccggctcc cgcgcccggc
     2161 agcggcaatt ctctgtgcag ggtcgcggag ctcgaccacg acgacgcgag cgactggagc
     2221 gtctgcccgc cgagccccgg cctcgcgaat gaccccgaag tcagcgacac cacgggtgat
     2281 atgagcggcg ctggggactc cgaggctacc ggtacgaccg gcgagccgct gcccgcggag
     2341 gtcgtgatcg tcgaggtcct cagcaacccg cccggccccg gcagcgagga gaagttcacc
     2401 gagttcatcg agatcgtgaa cctcggaccc ggtaccgtcg acctcgccac ctggacgatc
     2461 gccgatgccc tcgccgagca gccgaccggg gccgacccgc tgctgtacgc cgacggcgac
     2521 ggcggctgcg ccccggagac ctgcctggcc gccggccagc gggcgatcct cgtcgggaac
     2581 ctctacaacg gcccgaccgg acctggcctg gtgctcgtca ccgatgacac cacgctggcc
     2641 aacgcgggcc tggccgtcca tgagccagtg gcactacgcg atggtgaggg tgtcctgcgc
     2701 tcgacctacc gcgcctggcc ggatcctctc gtcgccccgg accccgcgct cagcgagcag
     2761 gcgctggttc gggcgagcgc cgaggcggcc gacgcagcgg aggcgtggag cttcgcagcc
     2821 ccgacgcctg gcctctagtc gaggtcgtag gacgggcgac cacgacgcgg cggacggcga
     2881 tggaagttgt cgcggagctc gacctctcgc atcagctcga tctcctcttt gccaagatca
     2941 tcaaggtctt gcagcgcctc gcgcttgaac tcctcgaagc tcgtgaactc agtcctgaag
     3001 tagctcatgt ccgccttcgt aatccatacc ccgacatgta ggcaagaaaa ctacgtcgcg
     3061 cctgcgacgg gctcgccgca aacgagcggc acggcgcggc gcctcggtgg taaggagtgg
     3121 gccgcgtgcc caaggacccc gcagcaacgg cccctccccg cgctcgccca ccggccggcg
     3181 cgcggatcgt ggcgatcagc aatcaaaagg gcggcgaggg caagaccac
//
LOCUS       DASWNJ010000543         2421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_222074, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000543 DASWNJ010000000
VERSION     DASWNJ010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222074"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VGB85_09165"
     CDS             complement(<1..715)
                     /locus_tag="VGB85_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134238.1"
                     /translation="MAGSPLPTGYGPGRRRIAPAHKLGIAVVIANLAGLIAAVYCAQQ
                     IRAVPAEASTETILFAVYWTALCLAAVVDALFIDELVFHGAFRLQHFSGKDGSRLNLK
                     DDEVVVAASMQRSSVSFPAALILCGGLTYLLFNVVNHDFNGYYRRIGKHVSALRGDAP
                     ETQAQRSQAIAALSIQPDRAEIIPLLLQQLGRGGETGAFAAWALGRLSDAKNFRKPII
                     EALWAASEEPDPAVRHEALI"
     gene            816..2420
                     /locus_tag="VGB85_09170"
     CDS             816..2420
                     /locus_tag="VGB85_09170"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEY0134239.1"
                     /translation="MSKLAAREAKPVRRASQQRIRLELAPAIVDAPEVGDAKAQGETI
                     APAAQSQAAPVTARRRRGLGTWLAAAEAVTMAAGGLAVAAMLWTGFDEARSDPRVLPG
                     VKLAGQDIGGRTADELPILAEAAAMASLDRKLVLVAPGVEVTTTARELGAVPSPEAAI
                     AAALGVGRSGDLLVDLRARARAQRGEVDLAVGHRFTEQEALDRLLALAPSVERPSLPT
                     RFDMENRRILPAQRGIRLSAFDSLSAVAVGLAAGAERIELVVAPAPPVEDPLASLAAG
                     LDIGTVLSSFSTPYSTEPNYADRAANLKVGAAALDGYVLMPGEVMSFNQVVGDRTDTA
                     GFRYAPGIADGELIDTVGGGICQISSTLYGAAFFAGLELSHSRPHSRPSSYVDMGLDS
                     TVVFGSVDMKLKNPFDFPVVLHTSVSAGKVKIEVLGRQRVYDEVAFERQVKEVLPHST
                     IVRDDPRLHTGAETVTQRGMRGFKVVRTRKLYKAGEIVDTQSWDLFYPPTTHIVRRGT
                     NPRGEYPESKPLPPLRDPARELRVVQ"
BASE COUNT          346 a          860 c          883 g          332 t
ORIGIN      
        1 cgatcagggc ctcgtggcgg acggccgggt cgggctcctc gctggcggcc cacagggcct
       61 cgatgatcgg cttgcggaag ttcttggcgt ccgagaggcg gccgagcgcc caggccgcga
      121 aggccccggt ctcgccgccg cgcccgagct gttgcaacag caaggggatg atctcggcgc
      181 ggtcgggctg gatcgacagg gcggcgatcg cctggctgcg ctgcgcctgg gtctccggcg
      241 cgtcgccacg cagcgcactg acgtgcttgc cgatccttcg gtagtaaccg ttgaagtcat
      301 ggttgacgac gttgaacagc aggtaggtca gcccgccgca caggatcaac gccgccggga
      361 agctgaccga cgagcgctgc atgctcgcgg cgacgacgac ctcgtcgtcc ttgaggttga
      421 gccgcgagcc gtccttgccg gagaagtgct ggagccggaa cgcgccgtgg aacaccagct
      481 cgtcgatgaa cagggcgtcg acgacggcgg cgaggcacag cgcggtccag tagacggcga
      541 agaggatcgt ctcggtcgag gcctcggccg ggacggcgcg gatctgctgg gcgcagtaga
      601 ccgcggcgat cagcccggcg aggttggcga tcacgacggc gatcccgagc ttgtgcgccg
      661 gggcgatgcg acggcgcccg gggccgtacc cggtggggag cggggagcct gccatgggtg
      721 cagtcataac ccaggaggac gcctgacggg gcccggggtt ccctcgcttt cgctggtttt
      781 tggacttgcc gcgcgcgccg tgcctccatg ccgccatgag caagctggcc gcgcgcgaag
      841 ccaagcccgt ccggcgggca tcccaacaac ggatccgcct cgagctggcg ccggcgatcg
      901 tcgacgcccc cgaggtcggc gacgcaaaag cgcagggcga gacgatcgcc cccgcggcgc
      961 agtcgcaggc cgcgccggtg accgcacgtc gtcggcgcgg gctcggtacc tggctggcgg
     1021 ccgccgaggc cgtgacgatg gcggccggcg gcctcgcggt cgcggcgatg ctgtggaccg
     1081 gcttcgacga ggcgcgcagc gacccccgcg tgctgcccgg cgtcaagctg gccggtcagg
     1141 acatcggcgg gcgcacggcc gacgagctgc cgatcctggc cgaggcagcg gcgatggcca
     1201 gcctcgaccg caagctcgtg ctcgtggccc ccggcgtcga ggtgacgacg accgcccgtg
     1261 agctcggcgc cgtgccgagc cccgaggccg cgatcgcggc ggcgctcggt gtcggtcgat
     1321 ccggcgatct gctcgtcgac ctgcgggcac gggcacgggc ccagcgcggc gaggtcgacc
     1381 tcgcggtcgg ccaccgcttc accgagcagg aggcgctcga ccgcctgctc gcgctggccc
     1441 cctcggtcga gcggccgagc ctgccgaccc gcttcgacat ggagaaccgc cgcatcctgc
     1501 cggcccagcg cgggatccgg ctgtcggcct tcgactcgct gtcggcggtc gcggtcggcc
     1561 tcgccgcggg cgcggagcgg atcgaattgg tcgtcgcgcc ggcgcccccg gtcgaggacc
     1621 cgctggcgag cctcgccgcg ggcctcgaca tcggcacggt gctgtcgagc ttctcgacgc
     1681 cgtactcgac cgagcccaac tacgccgacc gggccgccaa cctcaaggtc ggcgcggcgg
     1741 ccctcgacgg ctacgtgctg atgcccggcg aggtgatgtc gttcaaccag gtcgtcggtg
     1801 accgcacgga cacggccggc tttcgctacg cgcccggcat cgccgacggc gagctgatcg
     1861 acacggtcgg cggcggcatc tgtcagatca gctcgacgct gtacggcgcg gccttcttcg
     1921 ctggcctcga gctgagccac agccgcccgc actcgcgccc gagcagctac gtcgacatgg
     1981 gcctcgactc gacggtcgtg ttcggcagcg tcgacatgaa gctcaagaac cccttcgact
     2041 tcccggtcgt gctgcacacc agcgtcagcg ccggcaaggt caagatcgag gtgctcggga
     2101 ggcaacgcgt ctacgacgag gtcgcgttcg agcgccaggt caaggaggtg ctgccgcact
     2161 cgacgatcgt ccgcgacgat ccgcgcctgc acaccggcgc cgagacggtc acccagcgcg
     2221 gcatgcgagg cttcaaggtg gtgcgcacgc gcaagctcta caaggccggc gagatcgtcg
     2281 acacgcagtc gtgggacctg ttctacccgc cgacgaccca catcgtccgc cgcggcacca
     2341 atccgcgcgg cgagtatccc gagagcaagc cgctgccgcc gctgcgtgac cccgcccgcg
     2401 agctgcgggt ggttcagtga g
//
LOCUS       DASWNJ010000544         3397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_503863, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000544 DASWNJ010000000
VERSION     DASWNJ010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503863"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VGB85_09175"
     CDS             <1..228
                     /locus_tag="VGB85_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134240.1"
                     /translation="LLLCSDGLHEYLDATRTLHDLFAMDVQQAARQAIKHANDQGGRD
                     NITAVFVEYLGPGVAASPAAVAPPVNKSGAA"
     gene            522..1418
                     /locus_tag="VGB85_09180"
     CDS             522..1418
                     /locus_tag="VGB85_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134241.1"
                     /translation="MEPYWERIADPPPVPVASVEPVEPVEPVEPAGPVADADAKLPTP
                     ARFVVAASFGTSLGPPVYGWGELSLFLGVSLPRRARAWSFVALGTQTVYTPFLMYHQH
                     LTVHGVAGPKRGLLYSLGAGPVYYRDSSEAIVGGEVEGRIGWVFTQRPGARIRGVLGL
                     VARGGGVVQQDLRQRREEALWQVGAFVGFTRGPVGLEVRGRRPEDGAPPRGLGMLIPG
                     AIMLGMFGAVVLVDIAVLSSAPSGDEGGCHACVPLTVYALPALVPAIPLTVAGKLRNN
                     RYREWKARMTFAPRPTGFTMRF"
     gene            1483..2325
                     /locus_tag="VGB85_09185"
     CDS             1483..2325
                     /locus_tag="VGB85_09185"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY0134242.1"
                     /translation="MHALPEAYIGTAGWAIPKTAADGFPGDGTHLQRFARTLTGAEIN
                     SSFYRPHRPSTYAKWAASVPYGFRFAVKIPKEITHVRRLGELGTIEEILENFLAEAGA
                     LGDRLGPLLVQLPPSLAFVPAVAERFFTALRSRVAGPVVCEPRHASWFTASVGPRSAK
                     MGTRGATKVTGPQAMLAAFEVAQVAADPALSTAAARPGGWGGLAYYRLHGSPTIYRSP
                     YSPQYLDALARTLAGHLAAGTPVWCIFDNTAEGAAAADALGLLARLNMARPMPALAAQ
                     PPSL"
     gene            complement(2307..>3397)
                     /locus_tag="VGB85_09190"
     CDS             complement(2307..>3397)
                     /locus_tag="VGB85_09190"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0134243.1"
                     /translation="LPVMMGVIASVGAAMATGIFAMRGTGSVPGPAPFAVPGVQGVWS
                     APASAGHFTDRPMLAEVNGDGVLDVVGKVRGGGSMVEQITAFDGSNGAELWHTEPLTK
                     DASDSGTLRGVLLDRVISVDALGKAQAYDLRTGNPAWSVLLGEKGSKLCDAGGTIVIE
                     TTDDVRHGLDPVTGKKRELARNAACKPVFSSERDISPGYRIIDWPEMREIGMPNLSDI
                     DGITAHRGLVVEGDGPRFMLGQRSKGTSVPMVAAVDKKKKVLWMDVVPGVDPLTTDVN
                     TLGMEAAYIDGKLVVPYALKDSDAGTRMACFDATTGQRLWDVQALPEDDNWSGIAVSP
                     ESVFFTAWFDAGVVLSLETGELRYKLGG"
BASE COUNT          452 a         1188 c         1228 g          529 t
ORIGIN      
        1 ctgctgctgt gctccgacgg cctgcacgag tacctcgacg cgacgcggac cctgcacgac
       61 ctgtttgcga tggacgtgca acaggccgcc cgccaggcga tcaagcacgc gaacgaccag
      121 ggcggacgcg acaacatcac cgcggtgttc gtcgagtacc tcggccccgg cgtcgccgcc
      181 tcgccggccg cggtggcgcc accggtcaac aagtccggcg ccgcctgagc ggccgggcac
      241 ccggcgtcgc agaacgctgt atgtcgacgg cataaattgc acgcacaccg tggttccgcg
      301 cgcttgccgg ggcgccggga cccgggtatt gtggccccct attcgtatcg tgttcggtcc
      361 cggggccgga tcgctatatg atcgtatatg ggtatttatc ggccacgctg gccttcgcgg
      421 cgccgggcac cgcgacgtcg gtcgatccgg cgacgcgagg atatgacggt ccgacggcgt
      481 ccgaagggcc gactgtcgac attgtggccg agccggccgc ggtggaaccg tactgggagc
      541 gcatcgccga cccgccgccc gtgccggtcg cgtcggtcga gccggtcgag ccggtcgagc
      601 cggtcgagcc ggccgggccc gtcgcggacg cggacgcgaa gctaccgacg ccggcccgct
      661 tcgtggtcgc ggcgtcgttc gggacgagcc tcgggccccc ggtctacggg tggggcgagc
      721 tctcgctgtt cctcggcgtc agcttgccgc ggcgcgcccg ggcgtggagc ttcgtcgcgc
      781 tcggcaccca gaccgtctat accccgttcc tgatgtatca ccagcacctg acggtgcacg
      841 gggtcgcggg cccgaagcgc gggctgctgt attcgctcgg cgcgggcccc gtgtactatc
      901 gtgacagctc cgaggcgatc gtcggcggcg aggtcgaggg tcggatcggc tgggtgttca
      961 cgcagaggcc cggggcacgg atccgcggcg tcctggggct cgtggcgcgg ggcggcggtg
     1021 tcgtccagca agacctccgc cagcgacgcg aggaggcgct gtggcaggtc ggcgcgttcg
     1081 tcgggttcac gcgcgggccg gtcgggctcg aggtcagggg acgacgtcca gaggacggcg
     1141 ccccgccgcg gggcctcggc atgctgatcc ccggcgccat catgctcggc atgttcggcg
     1201 cggtggtcct cgtcgacatc gctgtgctct cctccgctcc ctcgggcgac gaggggggct
     1261 gccacgcgtg cgtcccgctg acggtctacg cgttgccggc gctggtgcct gcgatcccac
     1321 tgacggtcgc cggcaagctc cgcaacaatc gctaccgcga atggaaggcg cgcatgacct
     1381 tcgcgccacg gccgacgggc ttcacgatgc ggttttgaga agggcgggtc gggccgtgaa
     1441 acttgaacga cgcgcgatcc cggtccatag gcgtgggaag ccatgcacgc gctcccagaa
     1501 gcctatatcg gcaccgccgg gtgggcgatc ccgaagaccg cggcggacgg gttccccggg
     1561 gacggcacgc atctgcaacg gttcgcgcgg acgctgaccg gcgccgagat caactcgtcg
     1621 ttttatcgcc cgcatcggcc ctcgacctac gcgaagtggg cggcctcggt gccgtacggg
     1681 ttccgcttcg cggtcaagat cccgaaggag atcacgcatg tccgccggct cggagagctc
     1741 gggacgatcg aggagattct tgaaaatttc ctcgcggagg ccggcgcgct cggggaccga
     1801 ctcgggccct tgctggtcca gctgccgccg agcctcgcct tcgtgcccgc ggtcgcggag
     1861 cgcttcttca cggcgctgcg cagccgcgtc gccggcccgg tcgtctgcga gccgcgccac
     1921 gcgagctggt tcaccgcttc ggtggggccg aggtcggcga agatggggac acgcggcgcc
     1981 acgaaggtca ccggcccgca ggcgatgctc gcggcgttcg aggtcgcgca ggtcgccgcg
     2041 gatcctgccc tgtcgaccgc ggcggcgcgc cccggcgggt ggggcgggct ggcctactat
     2101 cgcctgcacg gctcgccgac gatctaccgc tcgccgtact cgccgcagta cctcgatgcg
     2161 ctggcgcgca cgctcgccgg ccacctcgcc gcgggcacgc cggtgtggtg catctttgat
     2221 aatacagccg agggggccgc ggccgccgac gcgctcggcc tgctcgcgcg actcaatatg
     2281 gcgcgaccga tgcccgcgct ggcagctcag ccgccgagct tgtagcgcag ctcacctgtc
     2341 tcgagggaca gcacgacgcc ggcgtcgaac cacgcggtga agaagacgct ctcgggcgac
     2401 acggcgatgc cgctccagtt gtcatcttcc ggcagggcct gcacgtccca caggcgctgg
     2461 ccggtggtgg cgtcgaagca ggccatgcgc gtgccggcgt cgctgtcctt gagcgcgtag
     2521 ggcacgacga gcttgccgtc gatgtaggcg gcctccatgc cgagcgtatt gacgtcggtg
     2581 gtgagcgggt cgacgccggg cacgacgtcc atccacagca ccttcttctt cttgtcgacg
     2641 gcggcgacca tcgggaccga cgtgcccttg gagcgctggc cgagcatgaa gcgcgggccg
     2701 tcgccctcga cgacgaggcc gcggtgggcg gtgatgccgt cgatgtcgga caggttcggc
     2761 atgccgatct cgcgcatctc cggccagtcg atgatgcgat agccgggcga gatgtcgcgc
     2821 tcgctgctaa acaccggctt gcacgcggcg ttcctggcga gctcgcgctt cttgccggtc
     2881 acggggtcga ggccgtggcg cacgtcatcg gtggtctcga tgacgatcgt gccgccggcg
     2941 tcgcataact tgctgccctt ctcgccgagc agcaccgacc acgcggggtt gccggtgcgc
     3001 aggtcgtagg cctgggcctt gccgagcgcg tcgacggaga tcacacgatc gagcagcacg
     3061 ccgcgcaggg tgccggagtc ggacgcgtcc ttggtcagcg gctcggtgtg ccacagctcg
     3121 gccccgttgc tgccgtcgaa ggcggtgatc tgctcgacca tgctgccgcc gccgcggacc
     3181 ttgccgacga cgtcgagcac gccgtccccg ttgacctcgg cgagcatcgg ccggtcggtg
     3241 aagtgcccgg cgctcgccgg ggccgaccac acgccctgca cgccgggcac ggcgaacggc
     3301 gccgggcccg ggacgctccc ggtgccgcgc atggcgaata tgccggtcgc catcgcggcg
     3361 ccgaccgagg cgatcacgcc catcatcacc ggcagca
//
LOCUS       DASWNJ010000545         5347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_468876, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000545 DASWNJ010000000
VERSION     DASWNJ010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468876"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="VGB85_09195"
     CDS             complement(<1..1004)
                     /locus_tag="VGB85_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134244.1"
                     /translation="MLRCLLVAALAAPPPASVGSPTTPTTPSTPGNAGMPAAASTAPA
                     TTPTPATATASTSTSPAVPPPVVTPVDPGPRGRLVLAATRPEGGPVTVRTRDGAQVAS
                     VQLQPGSRVQLDLPPGDYLIDDTARGGRITLSVEPDRIARFELTDAGARGSAGEGPPP
                     PRRTVSSHAPPRWKRTVSPVLSAVIPGLGQVVNGEPGKGVGILFGAVALAVSSAILRR
                     VPGHTDVSGRGLDSTSYGTEVISAAGYGLLSGGLQMLYAAQIMDAHATASGLRTPQPR
                     TRHRVAVELTRMATVGLRAGDPAAGFFPDWTVTVLGQVARRLSVGVSDLSVKQGHAFN
                     R"
     gene            complement(1044..1751)
                     /gene="aat"
                     /locus_tag="VGB85_09200"
     CDS             complement(1044..1751)
                     /gene="aat"
                     /locus_tag="VGB85_09200"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEY0134245.1"
                     /translation="MPVFLLPEDLPVFPDPRRAEAEGVLAIGGGLEPARVLMAYSRGI
                     FPWNSADEPLTWWCPAPRMVLQLPGDLHVPRTLRKQARRHPLRVSLDTDFAAVLGACA
                     SHPRPGQDGAWITPGIITVFTALHALGMAHSVEVWDGEALVGGLYGLAIGAVFFGESM
                     FSRASGASKHGFVALARQLVRWEFQLVDCQVASAHLRGLGGRELALAPFLDALAAGVR
                     APGRLGPWQIDPDIAGA"
     gene            complement(1755..2657)
                     /locus_tag="VGB85_09205"
     CDS             complement(1755..2657)
                     /locus_tag="VGB85_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134246.1"
                     /translation="MHTLSLRELSVVCALVCASACGDDKGDDTGTSTGATTSATATTG
                     GASMTDATSGTTDEPTTNTPTTMSSEPTTMSPDPTTDPTAGDGGQFCQESCAADADCT
                     IGGAAGFTCVDSRCHSSAPACTDEACRATFSNWVKVCATQAECPGQACINIGGGAGRC
                     ALVPSDLVMCAMFMQEEILMPVIEGGMDIGVCANTEYVCKDGSCQDPCEDNLSCVTAA
                     GHPQCNVGTGTCECTTDADCMNANKPGYAKCNAGVCGCGTDADCAGASNADVCTKDGY
                     CGCSSAAVCTTKAFDGTIPVCEGA"
     gene            complement(2736..4499)
                     /gene="glgP"
                     /locus_tag="VGB85_09210"
     CDS             complement(2736..4499)
                     /gene="glgP"
                     /locus_tag="VGB85_09210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEY0134247.1"
                     /translation="MFALTRKASASHDGSLMTAAAKDLIAYFSMEIALASEIPTYSGG
                     LGVLAGDTLRSGADMGLPMVAVTLLYRKGYFRQVLDANGTQSEKDVVWAPELHLERLR
                     DEVMIHIHGRDVRIGVWRYRVVGCDGHHVPVYLLDTDLEGNHADDRRISDHLYGFEER
                     YRLTQEAVLGIGGRQMLKALGLTPTRYHMNEGHSSLLAIDLLKEIWERSEDPTLEACL
                     AEVRSTCVFTTHTPIPAGHDRFPRALAEEVFGVDAVARVAEIPRLLTTELNMSILGLE
                     LSGFVNGVSVRHAEVSRGMFPGQEIHAVTNGVHSATWTAPPMQAVLDRHVPAWRRHGS
                     ALRSAQRIPVPEIVAAHRAAKQTLIDEINTLGDHRFEVDRFTIGFARRVTSYKRLMLI
                     LRDVGRLREIGRRWGSLQIVFSGKAHPRDQEGKRLIEAVYRQQEALLPYVRMVFVPGY
                     DMRVAGRIVAGVDLWLNTPMPPLEASGTSGMKAAHNGVPNFSVRDGWWCEGYIPGHTG
                     WVIEAAHSGDHAADQQDADRIYRCLDEEILPLYFSNQQRWGEMMRDCVALAASYFNTQ
                     RMLEEYATRAYGLSPVAVARR"
     gene            complement(4575..>5347)
                     /locus_tag="VGB85_09215"
     CDS             complement(4575..>5347)
                     /locus_tag="VGB85_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134248.1"
                     /translation="AVLDAIAMGLFDPTGPIATATLQGLDEHLRVDAGPGWSRNDDRW
                     DHANVEDLSPWGSDYDSAEWVITDLRGAIALRMAGQPERADALLAWVLEQSQKNYGMV
                     AETYDELTGAYKFNAPMLGFGAGAHALALAHRADATPDPACGAYYPEAPMPPGDSEGS
                     ETSTGDTITTGDASTGDATTEMAPTTGVTPTGSGGETAIDAGTGGPSTGTTSEDTDTG
                     PSGAGDAGCACREDPRQQGTAALLLLVSALLVRRRRAR"
BASE COUNT          715 a         1781 c         2087 g          764 t
ORIGIN      
        1 cggttgaagg cgtggccctg cttgacgctc aggtcggaga cgccgacgct gaggcgacgc
       61 gcgacctggc cgagcacggt gacggtccag tcggggaaga agcccgcggc cgggtcgccg
      121 gcgcgcagac cgacggtcgc catgcgggtc agctcgacgg cgacgcggtg gcgcgtgcgg
      181 ggctgcgggg tccgcaggcc ggaggcggtc gcgtgggcgt ccatgatctg cgcggcgtag
      241 agcatctgca ggccgccgct gagcaggccg tagccggcgg cgctgatcac ctcggtgccg
      301 tagctggtgc tgtcgaggcc gcgaccggag acgtcggtgt ggccgggcac gcggcgcagg
      361 atcgccgagc tgacggcgag cgcgacggcg ccgaacagga tgccgacgcc cttgcccggc
      421 tcgccgttga ccacctgacc gagcccgggg atcacggccg agagcaccgg cgagacggtc
      481 cgcttccagc gcggcggcgc gtgcgaggac acggtgcgac gcggcggcgg cgggccctcg
      541 ccggcgctgc cgcgcgcgcc cgcgtcggtg agctcgaagc gggcgatgcg gtcgggctcg
      601 accgagagcg tgatgcgacc gccgcgggcg gtgtcgtcta tcaagtagtc gccgggcggg
      661 aggtcgagct gcacgcggct gccgggttgc agctgcacgc tcgccacctg ggcgccgtcg
      721 cgggtccgga cggtgacggg gccgccctcg gggcgcgtcg cggcgagcac caggcgcccg
      781 cgcgggccgg gatcgaccgg cgtgacgacc gggggcggca cggccggcga ggtcgaggtc
      841 gaagcggttg cagttgcggg tgtcggtgtc gtcgcgggtg cggtcgacgc cgcggccggc
      901 atgccggcgt tgcccggggt ggacggggtg gtcggggtgg tcggggagcc gacgctcgcg
      961 gggggcggag cggcgagcgc ggcgaccagg agacagcgca gcacctggcg gttataggcg
     1021 gggacgtggt ccggcgtcca gggtcaggcg ccggcgatgt cggggtcgat ctgccaggga
     1081 ccgaggcgcc cgggggcccg gacaccggcc gcgagggcgt cgaggaacgg cgccagggcc
     1141 agctcccggc cgccgaggcc gcgcaggtgg gccgaggcga cctggcagtc gacgagctgg
     1201 aattcccagc ggaccagctg gcgggcgagc gcgacgaagc cgtgcttcga ggccccggag
     1261 gcgcgagaga acatgctctc gccgaagaac acggccccga tcgccaggcc gtagaggccg
     1321 ccgaccagcg cctcgccgtc ccacacctcg acgctatggg ccatgccgag cgcgtgcagg
     1381 gcggtgaaga cggtgatgat gccgggggtg atccacgcgc cgtcctgacc gggtcgcggg
     1441 tggctggcgc aggcgccgag caccgcggcg aagtcggtgt cgaggctgac gcgcagcggg
     1501 tgccgacgcg cctgcttgcg cagggtccgg ggcacgtgga ggtcgcccgg gagctgcaac
     1561 accatgcgcg gcgcggggca ccaccaggtc agcggctcgt cggcgctgtt ccacgggaag
     1621 atcccgcggc tgtaggccat cagcacgcgc gcgggctcga ggccgccgcc gatcgccagc
     1681 acgccctcgg cctcggcgcg gcgcgggtcc gggaacaccg gcagatcctc gggcagcagg
     1741 aacacgggca tggctcaggc gccctcgcac accgggatcg tgccgtcgaa ggccttggtg
     1801 gtgcacacgg cggccgagga gcagccgcag tagccgtcct tggtgcacac gtcggcgttg
     1861 ctggccccgg cgcagtcggc atcggtgccg cagccgcaca cacccgcgtt gcacttcgcg
     1921 tagcccggct tgttggcgtt catgcagtcg gcgtcggtgg tgcactcgca ggtgccggtg
     1981 ccgacgttgc actgcgggtg gccggccgcc gtcacgcacg acaggttgtc ctcgcagggg
     2041 tcctggcagg acccgtcctt gcagacgtac tcggtgttgg cgcagacgcc gatgtccatg
     2101 ccgccctcga tcaccggcat caggatctct tcctgcatga acatcgcgca catcacgagg
     2161 tcggagggga ccagcgcgca gcggcccgcg ccgccgccga tgttgatgca ggcctgcccg
     2221 gggcactcgg cctgggtcgc gcagaccttc acccagttgc tgaaggtcgc gcggcaggcc
     2281 tcgtcggtgc aggcgggcgc cgagctgtgg cagcggctgt cgacgcacgt gaagccggcg
     2341 gcgccgccga tggtgcagtc ggcgtccgcc gcacacgact cctggcagaa ctgcccgccg
     2401 tcgcccgccg tcggatcggt ggtcggatcc ggggacatcg tcgtcggctc cgaggacatc
     2461 gtcgtcggcg tgttcgtggt cggctcgtcg gtggtaccgc tcgtcgcgtc ggtcatcgac
     2521 gcgccgccgg tggtggcggt ggcggaggtc gtcgcgccgg tcgaggtgcc ggtgtcgtcg
     2581 cccttgtcgt cgccacaagc agaggcgcac acgagcgcgc aaacaaccga gagctcacga
     2641 agagacaggg tgtgcatgcg caagcagatg ccgcgaacct gccgccgtgt cgatgggaaa
     2701 tgtctgcatc gacccgtggg gccggtcgga ggcgctcagc gacgggcgac ggcgaccggc
     2761 gagaggccgt acgcgcgcgt ggcgtactcc tcgagcatgc gctgggtgtt gaagtacgag
     2821 gcggccagcg cgacgcagtc acgcatcatc tcgccccagc gctgttggtt gctgaagtag
     2881 agcggcagga tctcctcgtc gaggcagcga tagatgcggt cggcgtcctg ctggtcggcg
     2941 gcgtgatcgc cggagtgagc cgcctcgatc acccagccgg tgtggcccgg gatgtagccc
     3001 tcgcaccacc agccgtcgcg cacgctgaag ttgggcacgc cgttgtgggc ggccttcatg
     3061 ccgctggtgc cggaggcctc gagcgggggc atcggcgtat tgagccagag gtcgacgccg
     3121 gcgacgatgc gaccggcgac gcgcatgtcg tagccgggca cgaagaccat gcgcacgtac
     3181 gggagcaggg cctcctgctg gcggtacacg gcctcgatca ggcgcttgcc ctcctggtcg
     3241 cgcgggtggg ccttgccgga gaagacgatc tgcagcgagc cccagcggcg gccgatctct
     3301 cgcaggcggc cgacgtcgcg caggatcagc atcaggcgct tgtagctggt gacgcggcgg
     3361 gcaaagccga tcgtgaagcg gtcgacctcg aagcggtgat cgccgagcgt gttgatctcg
     3421 tcgatcaggg tctgcttggc ggcgcggtgg gccgcgacga tctcgggtac ggggatgcgc
     3481 tgggcggagc gcagcgcgct gccgtggcgg cgccaggccg gcacgtggcg gtcgagcacg
     3541 gcctgcatcg gcggcgcggt ccaggtcgcc gagtgcacgc cgttggtgac cgcgtggatc
     3601 tcctggccgg ggaacatgcc gcgcgagacc tcggcgtggc gcaccgacac gccgttgacg
     3661 aagccgctga gctcgaggcc caggatgctc atgttgagct ccgtggtcag caggcgcggg
     3721 atctcggcga cgcgggcgac ggcgtcgacg ccgaagacct cctcggcgag ggcccgggga
     3781 aaccggtcgt gtccggccgg aatgggcgtg tgcgtggtga acacgcaggt cgagcgcacc
     3841 tcggcgaggc aggcctcgag cgtcgggtcc tcgctgcgct cccagatctc cttgagcagg
     3901 tcgatcgcca gcagcgagga gtggccctcg ttcatgtggt agcgcgtcgg cgtcaggccg
     3961 agcgccttga gcatctgccg cccgccgatg ccgagcacgg cctcctgcgt gagccggtag
     4021 cgctcctcga agccgtacag gtgatcgctg atccggcggt cgtcggcgtg gttgccctcg
     4081 aggtcggtgt cgaggaggta gaccgggacg tggtggccgt cgcagccgac gacgcgatag
     4141 cgccacacgc cgatgcggac gtcgcggccg tggatgtgga tcatgacctc gtcgcgcagg
     4201 cgctcgaggt gcagctccgg ggcccacacg acgtcctttt ccgactgcgt gccgttggcg
     4261 tcgaggacct gacggaagta ccccttgcga tacagcaggg tcaccgcgac catcggcagg
     4321 cccatgtctg cgccgctgcg cagggtgtcg cccgcgagca cgccgagccc cccggagtag
     4381 gtcgggatct cgctcgccag cgcgatctcc atcgaaaagt acgcgatcag gtccttcgca
     4441 gcagccgtca tcagcgaacc atcatgcgac gcgctcgcct tgcgggtcaa ggcgaacacg
     4501 ggttgtccaa ggtttagcgc cgcatggacc gcgggtccgc gcgtgcgtcg atcgcccggt
     4561 cgatcgccgg tcgatcaccg cgcgcgacgg cgacgaacga gcagcgccga cacgagcaac
     4621 agcagcgctg ccgtgccctg ctgccgggga tcctcgcgac atgcacagcc cgcatcaccg
     4681 gcgccgctcg gtccggtgtc cgtatcctcg ctggtcgtgc cggtcgaagg tccgccggtg
     4741 cccgcatcaa ttgctgtctc gccgccggat cccgtcgggg tcacgccggt ggtcggtgcc
     4801 atctcggtcg tcgcgtcgcc ggtgctcgcg tcgccggtgg tgatggtgtc gccggtgctc
     4861 gtctcgctgc cctcgctgtc gccggggggc atgggcgcct cggggtagta ggcgccgcag
     4921 gccgggtccg gcgtcgcgtc ggcgcggtgg gccagcgcga gcgcgtgggc gcccgctccg
     4981 aagccgagca tcggcgcgtt gaatttgtac gcgccggtga gctcgtcgta ggtctcggcg
     5041 accatgccgt agttcttttg cgactgctcg agcacccagg cgagcagtgc gtcggcgcgc
     5101 tccggctgcc cggccatgcg cagcgcgatc gcgccgcgca ggtcggtgat cacccactcg
     5161 gcgctgtcgt agtcgctgcc ccaggggctg aggtcctcga cgttggcgtg gtcccagcgg
     5221 tcgtcgttgc gcgaccagcc cgggccggcg tcgacgcgca ggtgctcgtc gaggccctgc
     5281 aaggtggcgg tggcgatcgg gccggtcggg tcgaacaggc ccatggcgat cgcgtcgagc
     5341 acggcgg
//
LOCUS       DASWNJ010000546         7207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_645372, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000546 DASWNJ010000000
VERSION     DASWNJ010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7207
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645372"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VGB85_09220"
     CDS             <1..709
                     /locus_tag="VGB85_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134249.1"
                     /translation="QAVIAQLGEPALRPQWLTETPAKGVKAPRDLDSLFDFICAAQEQ
                     VGQASVEFTLVDLQPGLPPVPPGFAPIGDPTGHLLHSFARAEELVLVVAPQLFKVPSL
                     LLGHVARELGRLGLWHAPEQSPAVVALDSETAAELAGIALGMGTWVANGAYMFNNACC
                     GGGCGIDLGSVRAALSMPEACYALALDAQRKGLGRRHAAKVLESTQAAALKQSWDACA
                     PNIPALPAARNAGSLRA"
     gene            complement(788..2944)
                     /locus_tag="VGB85_09225"
     CDS             complement(788..2944)
                     /locus_tag="VGB85_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134250.1"
                     /translation="MRLTPSSWPTIPTWLWIVLIALVIVVLDVATIAACGAWDPWETH
                     YGEVARQILVRDDPIDLWWKPGYGPDGNAENTFWSKPALPFWLMAISMALLGVGSRPA
                     ADEMVRGVAPELALRLPSMLAGTLCAVFLGYVVWRLLREEGGAPARGPSPAARAGIFT
                     AVVLATMPQWAIVTRQALTDMFFVAPVVVAMGAWALAWMQPDRDLRRRAIGEVLRRPA
                     PATGGGAWGRVRRWTVPWDRAYTVFIVGTIVAAIVPLTVLHVHVISADTVARVAKFVK
                     KPGVPNIDTLRQIHLHMFFYWSAALVVLIASLRWARRSQVWMGVLYVAAGVSLMGKGM
                     IGPGIVGLLVLVDMIVRGRLDLLRRCGLMSGFLLFALASLPWHHAMAIYRGERWVNEL
                     IIVNNLARFGSGEQEQAVGSFAFYLRTLGLAAFPWSAAVPAALWSAMRRLRDDPEDMP
                     DAPGASGQVRAAALRRFALLWAAVTLALLSYSVTKYYHYLLPVLPPLAVLLGLWLGEL
                     RKGDAPDRRVAWVTAGAGIGVLGLVLRDVIVEPAWIAHLTTYLYDSMWRQGAPATDRL
                     LLLAVPFALGLLLWAAGRVRAAAASMLLSALLIFGWTLNGYLPAASEEWSQRSAIRTY
                     YDQRGPEDPLLSWWFYYRGETFYAKANVWVLKDNDREQLAEFIDTQRGHGRSLWIMTT
                     AGHSKRARTALPSDLRAGVTVAYENSHYALVRVPVP"
     gene            2995..4053
                     /locus_tag="VGB85_09230"
     CDS             2995..4053
                     /locus_tag="VGB85_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEY0134251.1"
                     /translation="MTAALSLDHVRDAAQRLAGKIWHTPVIRCPALEARAGRELFLKA
                     ENLQKIGAFKARGALHAVSRLPEEVRARGIVTYSSGNHAQAVALAAREFGVRAWIAMP
                     VDAPLIKVAGVRALGAEIVFAGTTSDDRHRAALELSRQHGAAIIEPFDDPDIIAGQGT
                     ATLELCEHVLATTGAPLDAIVVPVGGGGLIAGAGLVASALGIPLYSAEPRTCNAMARS
                     LAAGERVAVAPGPTIADGLKPIRVGALNLEVARRTISGAFEVEDSELGEALVRLLLDA
                     KILVEPSGAAALAVAMRGPALAVAARGPALAIAPRGSGPSPGNDPAGGFPGRRVGVLL
                     SGGNVSPALVAELLGKYA"
     gene            4083..5303
                     /locus_tag="VGB85_09235"
     CDS             4083..5303
                     /locus_tag="VGB85_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134252.1"
                     /translation="MRPVSRDLSKSAALWLACCGPAATPAPEATPAAPATAEQSADDD
                     ADAAEARRLAELPHRVVFADRITYSYVLLLAGSGVAAPTREELDAAVRKAFADNLEDE
                     EVQLLLGLVARPIREFGAPPTEAEALEEGAEDLLGFSVEAHPRTPVTGPALIESKVLA
                     DPDLTRELSPEERASLPARGSLLVLRAQYRNQHGVRGLRLLQTLVRVVATQRGALIHD
                     PDTLETHGPAAFAARRLQSSLGNVADQIPVVPFPDPRHTGRFRLATRGMRRFGSVDLE
                     LDGLPGELQTLQLATFFLHGLARMMIDLGEFESGFAAELPPTLTLHHSDCAAAYAGRP
                     GSLPRCKDCPESVDLHLVERAAEPQDAISHVVARVVAPRPRSDQGAYDHPQWVQEAIA
                     RVLGSPGPGGPAPM"
     gene            5576..7195
                     /locus_tag="VGB85_09240"
     CDS             5576..7195
                     /locus_tag="VGB85_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134253.1"
                     /translation="MTLALLLAACSSAGDVLSVENSGTTSAPDSSASAGPNDTIASSS
                     SDPASGEADGGQETGVASGEAGSSDSGEPSDPPQDPIPAEPSPEDVIDAKGLVIDEDV
                     CDLQTPVSIELRTLDAAATVSPTQARDAALSGWSSLTGIRIRPWEFFNYYAFDYPAAE
                     PGSVTITPQLALAPAQVDAASPEFLLQIGVGTQALAAAARPPMRLTLALDNSNSMTGK
                     AQDMLHATGKALAANLREGDTLSIVTWNSKYPTVLELHAITGPGDPVVLEKLDALELG
                     GSAELYSGLMSAYKLAVAGYDPLAWNRVLLVSDGGATANDTDLAVIRKHAEQQIYLSG
                     VGVGEAGIYRSDMIDAAAHAGRGASMFVGSEAEADKRFGPQLVRTLGTAVRDVSAHLE
                     LPPGFQVVRDPGGLNEFNADDPSVRLGPNASLVLHRRLRSCNPDAASDPEAKLLVRVT
                     FIDEATGELKEAKSAVKLSTLLADKPAALAKGAALAAYAAALQRWQARPADLAEALTE
                     AKERLKAAMDLLPNDPELAEISAVLDVLDAG"
BASE COUNT         1076 a         2889 c         2284 g          958 t
ORIGIN      
        1 ccaggccgtc atcgcccagc tcggcgagcc tgccctgcgc ccacagtggc tcaccgagac
       61 gccggccaag ggcgtcaagg ccccgcggga cctcgacagc ctgttcgatt tcatctgcgc
      121 cgcgcaggag caggtcgggc aagcctccgt cgagttcacc ctcgtcgacc tccagcccgg
      181 cctgccgccg gtcccgccgg gcttcgcacc gatcggtgac ccgaccggcc acctccttca
      241 tagcttcgcg cgcgccgagg agctggtgct cgtcgtcgct ccgcagctgt tcaaggtgcc
      301 ctcgctgctg ctcggtcacg tcgcccgcga gctcggtcgc ctgggcctgt ggcacgcgcc
      361 cgagcagagc cccgccgtcg tcgcgctcga ctccgagacc gccgccgagc tcgccggcat
      421 cgcgctcggc atgggcacct gggtggccaa cggcgcctac atgtttaata atgcctgctg
      481 cggcggcggc tgcggcatcg acctcggcag cgtgcgcgcc gccctctcga tgccggaggc
      541 ctgctacgcg ctcgctctcg acgcgcagcg caagggcctc ggccggcgcc acgccgccaa
      601 ggtcctcgag agcacccagg ccgccgcgct caagcagagc tgggacgcct gcgcgccgaa
      661 catcccggcc ttgccggccg cgcgcaacgc cggctccctg cgcgcctgag cctcgtcgat
      721 gccgcccgcg aagtcgggcc gcgtcacacc cacgcggcag cgatcccggg ctcaccgatc
      781 agccggatca gggcaccggc acccgcacca gcgcgtagtg gctattttca taggccaccg
      841 tgaccccggc ccgcaggtcc gacggcaagg cagtgcgggc ccgcttcgag tgcccggccg
      901 tcgtcatgat ccacagggag cggccgtgcc cgcgctgggt gtcgatgaat tccgcgagct
      961 gttcgcggtc gttgtccttg agcacccaga cgttggcctt cgcgtagaac gtctcgccgc
     1021 gataataaaa ccaccagctc agcagcgggt cctcgggccc gcgctgatcg tagtacgtgc
     1081 ggatcgccga ccgttgtgac cactcctcgg aggccgcggg caggtatccg ttgagggtcc
     1141 agccgaagat cagcagcgcc gacagcagca tgctcgccgc ggccgcgcgc acgcgaccgg
     1201 ccgcccacag caacagcccc agcgcgaacg ggaccgccag cagcagcagg cgatctgtcg
     1261 ccggcgcgcc ctgtcgccac atcgaatcat acagataggt cgtcaggtgg gcgatccacg
     1321 ccggctcgac aattacgtcg cgcagcacca gcccgagcac gccgatgccc gcgcccgcgg
     1381 tcacccacgc gacccgccgg tccggcgcgt cgcccttgcg cagctcgccg agccacagcc
     1441 cgagcagcac cgccagcggc ggcagcaccg gcagcaggta atgatagtac ttggtgacgc
     1501 tgtagctcag cagcgccagc gtcaccgccg cccacagcag cgcgaatctc cgcagcgccg
     1561 ccgcgcgcac ctgtcccgag gcacctggcg catcgggcat gtcctcgggg tcatctcgca
     1621 gcctgcgcat cgccgaccac agcgccgccg gcaccgcggc cgaccacggg aacgccgcga
     1681 gcccgagcgt acgcaggtag aacgcgaagc tgccgaccgc ctgctcctgc tcgccggacc
     1741 cgaagcgcgc caggttgttg acgatgatca gctcgttcac ccagcgctcg ccgcgataga
     1801 tcgccatcgc gtggtgccac ggcagcgacg cgagcgcgaa cagcaggaag ccgctcatca
     1861 acccacagcg ccgcagcaga tcgaggcgcc cgcgcacgat catgtccacc agcacgagca
     1921 gcccgacgat ccccggaccg atcatgccct tgcccatcag cgacacgccg gcggccacgt
     1981 agagcacgcc catccacacc tgcgagcgcc gggcccagcg caggctggcg atcagcacca
     2041 ccagcgccgc gctccagtaa aagaacatgt gcaggtggat ctgccgcagc gtgtcgatgt
     2101 tcggcacccc gggctttttt acaaatttcg ccacccgcgc gaccgtgtcc gcgctgatca
     2161 catgcacgtg cagcaccgtc agtggcacga tcgccgccac gatcgtcccg acgataaaaa
     2221 ccgtgtatgc gcggtcccac gggactgtcc agcggcgcac gcggccccac gcgccgccgc
     2281 cggtggcggg cgccggtcgt cgcaggacct ccccgatcgc gcgccgtcgc aggtcgcggt
     2341 ccggctgcat ccacgccagc gcccacgccc ccatggcgac caccacgggc gccacgaaga
     2401 acatgtccgt cagcgcttgc cgggtgacga tcgcccactg cggcatcgtc gcgagcacca
     2461 ccgccgtgaa gatccccgcg cgcgccgccg ggctcggtcc acgcgccggt gcgccgccct
     2521 cctcgcgcag cagtcgccac accacatagc cgaggaacac cgcgcacagc gtgcccgcga
     2581 gcatgctcgg caggcgcagc gccagctccg gcgcgacgcc acggaccatc tcgtcggcgg
     2641 ccggccgcga accgacgccg agcagcgcca tgctgatcgc catcaaccaa aacggcagcg
     2701 ccggcttcga ccagaacgtg ttctccgcgt tgccatcggg cccgtaaccg ggcttccacc
     2761 acaggtcgat cgggtcgtcg cgcaccagga tctgcctggc gacctcgcca tagtgcgtct
     2821 cccacgggtc ccacgcgccg cacgccgcga tcgtcgccac gtcgagcacg acgatgacca
     2881 gcgcgatcag cacgatccac aaccatgtcg gaatggtcgg ccaggaggat ggggtcaggc
     2941 gcacgtgccc gaggtgtacc gcagggcccg cgcaaaggtg tagccttctc gcgcatgacg
     3001 gccgccctct cgctcgatca cgtccgcgac gccgcccagc gcctggccgg caagatctgg
     3061 cacacccccg tgatccgctg ccccgcgctc gaggcccgcg ccggtcgcga gctgttttta
     3121 aaggccgaga atttacaaaa gatcggcgcc ttcaaggcgc gcggggccct gcacgccgtc
     3181 tcgcgtctgc ccgaggaagt gcgcgcccgc gggatcgtca cgtacagctc cggcaatcat
     3241 gcgcaggccg tcgcgctcgc cgcccgcgag ttcggcgtcc gcgcctggat cgccatgccc
     3301 gtcgacgcgc ctttaattaa agtcgcgggg gtgcgcgcgc tcggggcgga gatcgtgttc
     3361 gccggcacca cctcggacga ccgccaccgc gccgcgctcg agctgtcccg gcagcacggt
     3421 gccgcgatca tcgagccctt cgacgacccc gacatcatcg ccgggcaggg caccgccacc
     3481 ctcgagctgt gcgagcacgt gcttgcgacc accggcgccc cgctcgacgc gatcgtcgtg
     3541 ccggtcggtg gtggcggcct gatcgccggg gccggcctgg tcgccagcgc gctcggcatc
     3601 ccgctgtact cggccgagcc gcggacctgc aacgcgatgg cccgctcgct cgccgccggt
     3661 gagcgcgtcg ccgtcgcgcc gggcccgacg atcgccgatg gcctcaagcc gatccgcgtc
     3721 ggcgccctca acctcgaggt cgcgcggcga acgatctccg gcgccttcga ggtcgaggac
     3781 agcgagctcg gcgaggccct cgtgcgcctg ttgctcgacg ccaagatcct cgtcgagccg
     3841 tcgggcgccg ccgcgctcgc cgtcgccatg cgcgggcctg ctctcgccgt cgctgcgcga
     3901 gggcctgctc tcgccatcgc tccgcgaggc tctggcccca gccccggcaa cgacccggcc
     3961 ggcggcttcc ccggccggcg cgtcggcgtc ctgctctccg gcggcaacgt cagccccgcc
     4021 ctcgtcgccg agctgctcgg caagtacgcc tgatccacgc gctccacgtt catacctcac
     4081 tcatgcgacc tgtctcgcgc gacctgtcca aaagtgcagc cctgtggctc gcgtgctgcg
     4141 gcccggcggc cacccccgcc cccgaggcga cccccgccgc gcccgcgacc gccgagcaga
     4201 gcgccgacga cgacgccgac gccgccgagg ctcgccgcct cgccgagctg ccgcaccgcg
     4261 tcgtgttcgc cgaccgcatc acgtacagct acgtgctgct gctggccggc tccggcgtcg
     4321 ccgcaccgac gcgcgaggag ctcgacgccg cggttcgcaa ggcctttgcg gacaacctcg
     4381 aggacgagga ggtccagctg ctgctcggcc tcgtcgcccg cccgatccgc gagttcggcg
     4441 cgccgccgac cgaggccgag gcgctcgagg agggcgcgga agatctgctc ggcttctcgg
     4501 tcgaggccca cccacgcaca ccggtcacgg gcccggccct gatcgaatcc aaggtgctcg
     4561 ccgaccccga cctcacccgc gagctcagcc ccgaggagcg cgcctcactg cctgcgcgcg
     4621 gcagcctgct cgtgctgcgc gcccagtacc gcaaccagca cggcgtccgc ggcctgcgcc
     4681 tgttgcagac gctcgtccgc gtggtcgcca cgcagcgcgg cgcgctgatc cacgaccctg
     4741 acaccctcga gacccacggc cccgccgcct tcgccgcacg ccgcctgcag tcgagcctcg
     4801 gcaacgtcgc cgaccagatc cccgtcgtgc ccttccccga cccgcggcac accggacggt
     4861 ttcgtctggc gacgcgcggc atgcggcgct tcggcagcgt cgacctcgag ctcgacggac
     4921 tgcccggtga gctccagacg cttcagctcg ccaccttctt cctgcacggc ctggcccgca
     4981 tgatgatcga cctcggcgag ttcgagtccg gattcgccgc cgaattaccg ccgaccctca
     5041 ccctccatca cagcgactgc gccgccgcct acgccgggcg ccccggaagt ctcccgcgct
     5101 gcaaggactg ccccgagagc gtcgacctcc acctcgtcga gcgcgctgcc gagccccagg
     5161 acgccatctc ccacgtcgtc gcccgcgtcg tcgcgccgcg cccgcgcagc gaccaaggcg
     5221 cctacgacca cccgcaatgg gtccaggagg cgatcgcccg cgtgctcggt tcgccgggcc
     5281 ccggggggcc tgccccgatg tagtcgcgtg caggcaccaa atgcgtgccg acacgtcgag
     5341 gcccggcaaa atacgccgaa gcgtggcgtc gccgcacgga cctcgatata ctggctcctc
     5401 atccttactt tgacgcttca ccgcgtcctt aacctcgtcc cttgagttta ctggaaggtt
     5461 ccccatgccc tcctggtccc gagccgcaag gctccacccc acgacgcacc ccacgtcgcc
     5521 ggcggtcccc caccgccacc acgctccact cgcccgacgc gctcccactt tcgtggtgac
     5581 gctcgccctg ttgctcgccg cctgttcgag cgccggcgac gtgctctccg tcgagaacag
     5641 cggcacgacc tcggctcccg actcgagcgc ctccgcgggt cccaacgaca cgatcgcctc
     5701 gagcagcagc gatcccgcca gcggtgaggc cgacggcggc caggagaccg gcgtcgccag
     5761 cggcgaggct ggcagcagcg acagcggcga gccgagcgac ccgccgcagg acccgatccc
     5821 cgccgagccg agcccggagg acgtcatcga cgccaagggt ctcgtcatcg acgaggacgt
     5881 gtgcgacctc cagacgcctg tcagcatcga gctgcgcacg ctcgacgccg cggcgacggt
     5941 ctcaccgacg caggcccgcg acgcggcgct gagcggctgg tcgtcgctga ccggcattcg
     6001 catccggccg tgggagttct tcaactacta cgccttcgac taccccgccg ccgagcctgg
     6061 cagcgtcacg atcacaccgc agctcgcgct cgcgccggcc caggtggacg ccgcgagccc
     6121 cgagttcctg ctgcagatcg gtgtcggcac ccaggcgctc gccgccgccg cccgcccgcc
     6181 gatgcgcctg acgctcgcgc tcgacaattc gaactcgatg accggcaagg cgcaggacat
     6241 gttgcacgcg accggcaagg cgctggcggc caacctgcgc gagggcgaca ccctctcgat
     6301 cgtcacctgg aactcgaagt acccgaccgt gctcgagctg cacgcgatca ccggccccgg
     6361 agatccggtc gtgctcgaga agctcgacgc cctcgagctc ggcggcagcg ccgagctcta
     6421 cagcggcctg atgtccgcat acaagctcgc cgtggccggc tacgacccgc tcgcatggaa
     6481 ccgcgtgctg ctggtcagcg acggcggcgc gaccgcgaac gacaccgacc tcgccgtgat
     6541 ccgcaaacac gccgagcagc agatctatct ctcgggcgtc ggcgtcggcg aggccggcat
     6601 ctatcgcagc gacatgatcg acgccgccgc ccacgccggt cgcggcgcct cgatgttcgt
     6661 cggcagcgag gccgaggccg acaagcgctt cggcccccag ctggtccgca ccctcggcac
     6721 cgccgtccgc gatgtctccg cgcacctcga gctgcccccc ggcttccagg tcgtccgcga
     6781 ccccggcggc ctcaacgagt tcaacgccga cgaccccagc gtccgcctcg gacccaacgc
     6841 cagcctcgtg ttgcaccgcc gcctgcggag ctgcaacccc gacgccgcgt ccgacccgga
     6901 ggccaagctg ctggtgcgcg tcaccttcat cgacgaggcg accggcgagc tcaaggaggc
     6961 caagtcggcc gtcaagctct cgaccctgct cgccgacaag ccggccgcgc tcgccaaggg
     7021 cgccgcgctc gcggcctacg cggccgcgct gcagcgctgg caggcgcgcc cggcggacct
     7081 cgcagaggcg ctgaccgagg cgaaggagcg gctcaaggcg gcgatggatc ttctgccgaa
     7141 cgaccccgag ctggccgaga tctcggccgt gctcgatgtc ctcgacgccg gctgagcgtg
     7201 cgcgatc
//
LOCUS       DASWNJ010000547         1661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_575142, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000547 DASWNJ010000000
VERSION     DASWNJ010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575142"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(686..>1661)
                     /locus_tag="VGB85_09245"
     CDS             complement(686..>1661)
                     /locus_tag="VGB85_09245"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY0134254.1"
                     /translation="PAASLAILHGGSLACTVNGTVLDHIRAPAMIGETALFVTGAHQT
                     ATLTADEATVVISMSSAGLRRLRAEANPLYAAILEHALVSTLRRVQQLDRQIAQFRQG
                     NFAAPSAPATEPLIARLWSRLRPPPQPDRATCPPLDPLLARQPILAAQPEARGALASA
                     FTAQYFRRGDSVARQGTVDPRAFVLAAGRLDALRTVEERGAAILLARFEPGAVFGVQA
                     LIEGGPRGASIVATTDGWLYHIDHAAHARLAPEVRTAWMETMLSVLSSQCATACLTLQ
                     GALVAFATNHPEALPSMVVHPRSDAGELDAADSFVVLRNSPPPSRRRR"
BASE COUNT          260 a          587 c          612 g          202 t
ORIGIN      
        1 ctcgccgctc gtgcgcggcg gtggggcgtc tgcgcgcgag ccctgggtcc tcccgctgct
       61 tcccccgcca gccggcccca cccgggcaca caccgtatgc gccccgcttg tcggtgcccg
      121 gatatcgtcg agcagccgac caacaccgta tgcgccccga ttgtcgctgc ccagataccg
      181 tcgagcaacc gaccaccagc ttcgtgggtt cgattgtctt gctcgctatg aacataccgg
      241 ccggcacgcg ctcacccgcg agacgccggt cgagcgacgg cacgcatgtt ttgacgagcg
      301 gggcgcagac aatgtccgcc cggggagcag gggccgacga gcgggggaag cagcgggagg
      361 acccaggggt cacgcgcaga cgccccgtca acttccgatc acgcgaagaa gtcttggcgt
      421 aagcgatatg ttatgcgcga cagatggggc ggcgagcatg tcccctggga ggacccgcgt
      481 cgcagggacc cgccgtccgt cggcccctcc tccccggccc atctagcctc gccccgcgct
      541 cccgatcacg ccaagccctg cccgcacacg cacgccaagc cgtgctcgca aacacgccaa
      601 gccctgcccg cacacgcacg ccaagccgtg ctcgcaagca cgccgagctc tgcccgcaca
      661 cgcaagccga gccgagccga gcgactcagc gacgacgacg cgaaggcggc ggcgagttcc
      721 gcaacacgac aaagctatcc gccgcatcga gctcccccgc atcgctccgc ggatggacga
      781 ccatcgacgg cagcgcctcg ggatggttgg tcgcaaacgc gacgagggct ccctgcaggg
      841 tcaagcaagc ggtggcgcac tgcgacgaga gcaccgagag catcgtctcc atccacgcgg
      901 tgcggacctc gggggcgagg cgggcgtggg ccgcgtgatc gatgtggtag agccagccgt
      961 cggtggtggc gacgatcgag gcgccgcgcg ggccgccctc gatgagggct tgcacgccga
     1021 acaccgcgcc gggctcgaag cgggccagca ggatcgccgc gccgcgctcc tcgaccgtgc
     1081 gcagggcgtc gaggcggccg gcggcgagca cgaaggcgcg ggggtcgacg gtcccctggc
     1141 gggcgacgct gtcgccgcga cgaaagtatt gggccgtgaa cgccgaggcg agcgcgccac
     1201 gggcctccgg ctgcgccgcg aggatcggct ggcgggcgag cagcgggtcg agcggcgggc
     1261 aggtggcgcg gtcgggttgg ggcggcggcc gcaggcggct ccacaggcgc gcgatgaggg
     1321 gctcggtcgc cggcgccgag ggggcggcga agttgccctg acgaaactgc gcgatctggc
     1381 ggtcgagctg ttgcacacgg cgcagcgtgc tgacgagcgc gtgctcgagg atcgcggcgt
     1441 acagcgggtt cgcctcggca cgcaggcgac gcaggccggc ggacgacatg ctgatcacca
     1501 cggtcgcctc gtcggcggtc agcgtcgcgg tctggtgcgc gccggtgacg aacagcgccg
     1561 tctcgccgat catcgccggg gcgcggatgt gatcgaggac cgtgccgttg accgtgcacg
     1621 ccagcgatcc gccatgaagg atcgccagcg aggcggcggg g
//
LOCUS       DASWNJ010000548         3062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_292878, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000548 DASWNJ010000000
VERSION     DASWNJ010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292878"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VGB85_09250"
     CDS             <1..685
                     /locus_tag="VGB85_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134255.1"
                     /translation="SFPSPGAPDDGFTKGRHLQSSGIEVVQSKRVTIADTTLGFAENR
                     GDAGNGYLFEVSQSSEVLVRDCVGEAGRHNFIQNWGFGATGIVWQRIHSKGGVSVAVM
                     DLDFGLPGLSEFHHSLATANLIDQSTLDDGWGAVNRNAYSSGAGHSATQSAIWNPDGA
                     GVIRSRQFGHGYVIGPHEKLKIETSIGGADAKGSEPEDFVEAIPADAPGPLTPASLYD
                     DQLARRLGK"
     gene            720..1418
                     /locus_tag="VGB85_09255"
     CDS             720..1418
                     /locus_tag="VGB85_09255"
                     /inference="COORDINATES: protein motif:HMM:NF025580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HEY0134256.1"
                     /translation="MSERNLFTAPTAALEREGPGGVRLPVLRYQLLIGVAGLGAVVSV
                     AELALCVLLSLPVLEGLEDSGALVRMWRPGGLDLVTWLSWAAVGSLALLGVTAGLFLA
                     WLVRARRNAAALGMSPRVPGALLWWFVPVANLLAPFVAVRGVYQASRGGGSRAPGWLV
                     TWWLVWLATLLWVCIEVLFARNVEAVHFVFSDIVGVVLALGLAAQGALARPLLIAVVV
                     AIQRAQQERRGVAR"
     gene            1827..>3062
                     /locus_tag="VGB85_09260"
     CDS             1827..>3062
                     /locus_tag="VGB85_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134257.1"
                     /translation="MPTDTGEVPTGDAPTTEPYPGCDGVDLAQDGILDLDVPAVKYIE
                     ISGGITVNGGALPDASGSRGQLQFDYTPAIGAPGSVGYALEATGPENYSVVLPAGVVT
                     VRWVPDAALCAEELGGPLPCTGGTVAEELAVNASGVLDLDIPSIVASGKLTQDGGVLP
                     DADGDRGHLEFSRPVGDMPGLVVPGGGVATKSFEASGAATYEVALFPDTYDIAFVGNP
                     GLCSEGAAPVPCNRGVVREAIALQATGVLDVDVRRVQVGGAVKVNGAAIGAGEADRGS
                     LRFDPADAVGGGISTPAFDVDGPVDYAISLVAGKYTVALTANPGLCGGDMPPAPTPCI
                     GGPVLPAIDLSASGVLDVDIPMIEVSGNVTLAGGVLPTEDGDRGSVSFARESGDATAV
                     PLETDGPIKYRLGLIPGEYT"
BASE COUNT          410 a          934 c         1230 g          488 t
ORIGIN      
        1 gtcgttcccc tcgccgggcg cgccggacga tggtttcacg aaggggcggc acctgcagtc
       61 gagcgggatc gaggtcgtgc agtccaagcg ggtgacgatc gccgacacga ccctcgggtt
      121 cgcggagaac cgcggcgacg cgggcaacgg ctatctgttc gaggtcagcc agagcagcga
      181 ggtgctggtg cgcgactgcg tcggcgaggc cggacggcac aacttcattc agaactgggg
      241 ctttggcgcg accgggatcg tgtggcagcg gatccacagc aagggcggcg tgtcagtcgc
      301 ggtgatggac ctcgacttcg ggctgccggg gctgtcggaa tttcatcact cgctggcgac
      361 cgcgaacctg atcgatcaga gcacgctcga cgacggctgg ggcgcggtca atcgcaatgc
      421 atacagcagc ggcgcgggcc acagcgcgac gcagagcgcg atctggaacc cggacggcgc
      481 cggcgtgatc cgctcgcgcc agttcggcca cgggtatgtg atcgggcccc atgagaagct
      541 gaagatcgag acctcgatcg ggggcgcgga cgccaagggc agcgagcccg aggattttgt
      601 ggaggccatc cccgcggacg cgccgggacc gctgacgccg gcgtcgctat acgacgatca
      661 gctggcgcgc cggctcggga agtgagccgg gcgagcccgc gagcgggtac catgcggcca
      721 tgagcgagcg aaacctgttc accgcgccga cggccgcgct cgagcgcgag gggccgggcg
      781 gggtgcgctt gccggtcctg cgctatcagc tgttgatcgg tgtcgcgggg ctcggggcgg
      841 tcgtcagcgt ggccgagctg gcgctgtgcg tgctgctgtc gctgccggtg ctcgaggggc
      901 tcgaggacag cggggcgttg gtgcggatgt ggcggcccgg cgggctcgac ctggtcacgt
      961 ggctgtcgtg ggccgcggtc ggctcgctgg cgctgctggg ggtcacggcg gggctgttcc
     1021 tcgcgtggct ggtccgggcc cgccgcaacg cggcggcgct cggcatgtcg ccgcgggtgc
     1081 ccggggcgct gctgtggtgg ttcgtgccgg tcgccaattt gctggcgccg ttcgtcgcgg
     1141 tgcgcggggt gtatcaggcg agccgcggcg gcgggtcacg cgctccgggc tggctggtga
     1201 cgtggtggct ggtgtggctg gcgaccttgc tgtgggtgtg tatcgaggtg ctgttcgcgc
     1261 gcaacgtcga ggcggtgcac ttcgtgttca gcgacatcgt cggggtggtg ctggcgctcg
     1321 ggctcgcggc ccagggggcg ctggcgcgtc cgctgttgat cgcggtcgtg gtcgcgatcc
     1381 agcgagcgca gcaggagcga cgcggcgtgg cacgataagc gaggtggtcg ccggtacgac
     1441 atcgcggcag gtcgcgtcgt acatggatct ccgcggatac ggcgtggcgc tgcggcgtgc
     1501 cagccggatc ggcgttcggg ccgggccgcg agctcgctgt gaacgtcgta atacatcgcg
     1561 gtcggccgcg ctggagttgc gagtcgacct cgcggacgcg taatcgcgcc ggagattcgt
     1621 acaaatttcg tcgaccctgc cgaccaatta aagatagggt cgggcccgga ttagatcctg
     1681 tgatgtatcc agtacgatgg ttccgacgtg gaggcctggt gtgccttccc ctgctgatgc
     1741 cggtttgcct gtgggggtgc ggggacaatt cctcggccga tgacacgtcg gccgcgacga
     1801 cgaccgggtc gaacgatacg ggggaggtgc cgaccgatac gggggaggtg ccgaccggcg
     1861 acgcgccgac gaccgagccg tatcccggct gtgacggcgt cgacctggcg caggacggga
     1921 tcctcgacct cgacgtgccc gcggtgaagt acatcgagat cagcggcggc atcacggtca
     1981 acggcggggc gctgcccgat gcgtcggggt cacgcggaca gctacaattc gactatacgc
     2041 ccgcgatcgg cgcgccgggc agcgtcggct acgcgctcga ggcgaccggg ccggagaatt
     2101 attcggtggt gttgccggcc ggggtcgtca cggtgcgctg ggtgcccgac gcggcgctgt
     2161 gcgccgagga gctcgggggg ccgctgccgt gcacaggtgg aacggtggcc gaggagctgg
     2221 cggtcaacgc ctcgggggtg ctcgatctcg acatcccgtc gatcgtcgcc agcggcaagc
     2281 tgacgcagga cggcggggtg ctgccggacg cggacggcga ccgcggtcac ctcgagttta
     2341 gccgcccggt cggcgatatg cccgggctcg tggtccctgg cgggggggtg gcgaccaaga
     2401 gcttcgaagc gagcggggcg gcgacctacg aggtcgcgct gttccccgac acctatgata
     2461 tcgcgttcgt cggcaacccc gggctgtgca gcgagggcgc ggcgccggtg ccgtgtaatc
     2521 gcggggtggt gcgcgaggcg atcgcgctgc aggcgacggg tgtgctcgat gtcgacgttc
     2581 ggcgggtcca ggtcggcggg gcggtcaagg tcaacggggc ggcgatcggc gccggtgagg
     2641 ccgaccgcgg gtcgctgcgc ttcgacccgg cggacgcggt cggcggtggg atctcgacgc
     2701 cggcgttcga tgtcgacggg ccggtggatt atgcgatcag cctggtcgcc gggaagtaca
     2761 cggtcgcgct gacggccaac ccggggctgt gtggcggcga tatgccgccg gcgccgacgc
     2821 cgtgcatcgg cgggccggtg ctcccggcga tcgatctatc ggcgtcgggc gtgctcgacg
     2881 tcgatatccc gatgatcgag gtcagcggca acgtgacgct cgccggcggg gtgctgccga
     2941 ccgaggacgg cgaccgcggc tcggtgtcgt tcgcgcgcga gagcggggac gcgaccgcgg
     3001 tgccgctcga gactgacggg ccgatcaagt atcgcctcgg cctgatcccc ggcgaatata
     3061 cg
//
LOCUS       DASWNJ010000549         4124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_468911, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000549 DASWNJ010000000
VERSION     DASWNJ010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468911"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(631..1869)
                     /gene="ftsY"
                     /locus_tag="VGB85_09265"
     CDS             complement(631..1869)
                     /gene="ftsY"
                     /locus_tag="VGB85_09265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEY0134258.1"
                     /translation="MPLELLVILIVLGTLVLVGAVIVVVRRQRALPPSPEHKPLAPGA
                     EPPRAVEGPVEPRRDRPLRPTGDVRDGPEIPLPVEKPRPEPRTPADEARLAERRRYEL
                     GLAKTRGGFVARLATLFRGKPRVSSELKEEVEQVLFTADIGAKTAQKLLDQVTALLDR
                     SQVADPEQVWAVIRREAREILGQSGPAVTYSPDPGPYVLLMIGVNGVGKTTTLGKLAA
                     YHQAAGRKVLLVAGDTFRAAAGEQLEIWARRVGCAIHQGKDGGDPSAVIFEGILRGAR
                     EGFDVILCDTAGRLHTKKELMDELQKIRRACAKALAKAHGKPESEVRGPHDTFLVLDA
                     TIGQNALAQAVLFKETMDFTGLVLTKLDGTAKGGVVIGVVDELKVPVRFIGIGEAIDD
                     LRAFDADAFCDALFSDDSRT"
     gene            2098..2301
                     /locus_tag="VGB85_09270"
     CDS             2098..2301
                     /locus_tag="VGB85_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134259.1"
                     /translation="MEERKQALAVATVEAQHQLGERGRREVSIQLGEAALLEDVFQLP
                     QAVAPQREQPGRELIGDGSRSMS"
     gene            2892..>4124
                     /locus_tag="VGB85_09275"
     CDS             2892..>4124
                     /locus_tag="VGB85_09275"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0134260.1"
                     /translation="MRIKKIEISGFKSFADREVVHIDDHVTAIIGPNGCGKSNIVDAM
                     RWCLGEQRAKHLRGAGMADVIFAGCSVRGPGNAAEVTLTFENAGNVPVAYLNYAEIAV
                     TRRLLRDGTSEYLINKVPCRLRDISELMTGTGAGTRGYSIIEQGQVGRIVSSKPEERR
                     YIIDEAAGITRFKAQKQAAEAKIAQTQQNLLRVRDVLGELEGRLGNLRRQAQKAERYK
                     RYRSELRALDLWMASHRFLELTMTARVLEARRTDLGEQVQAMRSALASHEARGEAARL
                     AASQAEQALSERQQQVFDLENRVRLAETEEQYRRREQEQLRTAATQARAEASVLARSL
                     ESLEQELAQVQAQYEEVLESGDEGHADTAERMQEEHDSLAARLRGEVSEHERRRSELS
                     RLHSREASVAAQLGARAEG"
BASE COUNT          662 a         1399 c         1534 g          529 t
ORIGIN      
        1 cagcaggagc agggccggca aacatcgccg tggaagccat cgcggggatc gcaacggcag
       61 cggatgcaga agcagggatc gcccaggctg tagcgaacag tacagccgag ccggtcaaga
      121 atcgtcgcat gctcacctca aggtctacgg gcacggaagt agctcactgg ggtagctggg
      181 gagggagagc tcgggagccc gcggagccgg gccacggcat ctacatcttc tgtccctcgg
      241 gacatcggtc tgtagcggtg tcggacggta tcacagcgaa ccactaggta acaacaggac
      301 aaagcagcgg cctcggcgaa gtttcgggtg gagcaaaaac acgcgtccaa acggccgtca
      361 gagccgctcc agcggccccg ggggctcaag gcgtccgccc agcgacgccg cgacaccaca
      421 acacagcacc gcagcgaccg gcgtgaccgg gcctgcacgc catgaagaat cggcgatgtc
      481 gcggcgatct cctgcgccgc tgcggcccgt tctttggcgg cgtacgggcg aaattgcgag
      541 tgtgccaaac acggtaaagg tccgcacaat gaccgccgtg gggcgctgcg ggtcgccctg
      601 gcacatgtcc tcccggacat gcaccaaagg tcaggtgcgc gagtcgtcgg agaacagggc
      661 gtcgcagaag gcgtcggcgt cgaaggcccg caggtcgtcg atcgcctcgc cgatgccgat
      721 aaagcgcacc ggaaccttga gctcgtcgac gacgccgatg acgaccccgc ccttggcggt
      781 cccgtcgagc ttggtcagca cgaggccggt gaagtccatc gtctccttga agaggacggc
      841 ctgggcgagc gcgttctggc cgatcgtcgc gtcgagcacg aggaaggtgt cgtggggtcc
      901 gcggacctcc gactcgggct tgccgtgggc cttggcgagc gccttggcgc aggcgcggcg
      961 gatcttctgc agctcgtcca tcagctcctt cttggtgtgg agccgaccgg cggtgtcgca
     1021 gaggatgacg tcgaagccct cgcgggcgcc acggaggatg ccctcgaaga tgaccgcgga
     1081 cgggtcgccg ccgtccttgc cctgatggat cgcgcagccg acccggcggg cccagatctc
     1141 gagctgctcg cccgcggccg cgcggaaggt gtcgcctgcc accagcagga ccttgcgacc
     1201 cgcggcctga tggtaggcgg cgagcttgcc gagggtcgtc gtcttgccga cgccgttgac
     1261 gccgatcatc agcaggacgt acggcccggg gtccgggctg tacgtgactg cagggccgct
     1321 ctggccgagg atctcccgcg cctcgcggcg gatgacggcc cacacctgct cggggtcggc
     1381 gacctggctg cggtcgagca gggcggtgac ctggtcgagc aacttctgcg ccgtcttggc
     1441 cccgatgtcg gcggtgaaca ggacctgctc gacctcctcc ttgagctcgc tgctgacgcg
     1501 gggcttgccg cggaacaggg tcgcgaggcg ggcgacgaag ccaccacggg tcttggcgag
     1561 gccgagctcg tagcggcggc gctcggcgag gcgggcctcg tcggcgggcg tgcgcggctc
     1621 gggccgcggc ttctcgaccg gcagcgggat ctccgggccg tcacggacgt caccggtggg
     1681 gcgcagcgga cggtcgcgac gcggctcgac ggggccctcg acggcgcgtg gcggctcggc
     1741 gccgggcgcc agcggcttgt gctcgggcga gggcggcagg gcgcgctggc ggcgaaccac
     1801 gacgatgacc gccccgacca gcacgagggt gccgaggaca atgaggatca cgagcagttc
     1861 gagcggcatc ggcgagggca gtctggtcgc acaggcggag gtcacgcaag gccccgggcg
     1921 ccggacgcag cgtgaggcga gatcccgcgc gatctgcgtt caggaggccg gcggttccgg
     1981 gggcgccggc ggtgccggcg agaggaacgc tgcggaccat cctggtcgac gcgcgtgcct
     2041 ggggacgccc cgcgccgacg tggtcatgtc gacgacagcg cgccgagcag gatctgcgtg
     2101 gaggagcgca agcaggcgct cgccgtcgca acagtggaag cgcagcatca gctcggcgag
     2161 cggggtcgac gcgaagtcag catccaactc ggtgaagctg ccctgctcga ggatgtgttc
     2221 caactgccgc aggctgtcgc tccacagcgg gagcagccag gtcgtgagtt gatcggggat
     2281 ggctcgcgat cgatgagctg acagagctct gcccgccacg cgggtccgac gatcgccgcg
     2341 agctccgtga gcgccgcgtc gtgcaggtgc tgtccggaac tatcgatgac ggcctgcagg
     2401 tgcccgtcct gcacggaggc gagcgcctgc gccgagtatt cgatcctgag tatgccggcg
     2461 tcgagcgcgg cgagcagctc gtcgcgcgtc gtgcaacgga gtgcggggcc gcgaggtcga
     2521 tgaccctcgc gccgagctcc gccagctctg cgtactcctg gaacgagtcc gactccgtgg
     2581 ccgggtccgc gtgcacggcc ggccaatcta cgagggcgtc cgggggcgtg caaggcgagc
     2641 ccgcgcgtga caccggctgc ccgggcggag gtcacgccag ggccctcgcg ccgcgacgcg
     2701 gcgtgaggcg agatcccggg cgatccccgg accgggcccg cggcgcccgg cgggtgcggc
     2761 aagggcgacg cagatcggcc cgggcggggc gtttcgcagg ggctgtggat aactcgcggc
     2821 cgtccggggg attctcgaaa agcctgacag gtgctatcgg gtgggcattc gcggcccggg
     2881 ccgccctcgc gatgcgcatc aagaagatcg agatcagcgg gttcaagtcg ttcgccgacc
     2941 gcgaggtcgt gcacatcgac gatcacgtca ccgcgatcat cgggcccaac ggctgcggca
     3001 agtcgaacat cgtcgacgcg atgcgctggt gtctcggcga gcagcgggcc aagcacctgc
     3061 gcggcgcggg catggccgac gtcatcttcg ccggctgcag cgtgcgcggg cccggcaacg
     3121 cggccgaggt gacgctgacc ttcgagaacg ccggcaacgt gccggtcgcc tacctcaact
     3181 acgccgagat cgcggtgacc cgccggctgc tgcgcgacgg caccagcgag tacctgatca
     3241 acaaggtgcc gtgccgcctg cgcgacatct cggagctgat gaccggcacc ggcgcgggca
     3301 cccgcggcta ctcgatcatc gagcagggcc aggtcggtcg catcgtcagc agcaagccgg
     3361 aggagcgccg ctacatcatc gacgaggcag ccggcatcac ccgcttcaag gcccagaagc
     3421 aggccgccga ggcgaagatc gcccagaccc agcagaacct gctgcgcgtg cgcgacgtgc
     3481 tcggcgagct cgagggccgc ctcggcaacc tgcgccgcca ggcccagaag gccgagcgct
     3541 acaagcgtta ccgcagcgag ctgcgcgcgc tcgacctgtg gatggccagc caccgcttcc
     3601 tcgagttgac gatgaccgcc cgcgtgctcg aggcccgccg caccgacctc ggcgagcagg
     3661 tccaggccat gcgcagcgcg ctcgccagcc acgaggcgag gggcgaggcg gcgcggctcg
     3721 ccgcgtcgca ggccgagcag gcgctcagcg agcgccagca gcaggtcttc gacctcgaga
     3781 accgcgtccg cctcgccgag accgaggagc agtaccgccg ccgcgagcag gagcagctgc
     3841 gcaccgcggc gacccaggcg cgcgccgagg ccagcgtgct cgcccgcagc ctcgagagcc
     3901 tcgagcagga gctcgcccag gtccaggccc agtacgagga ggtcctcgag agcggcgacg
     3961 agggccacgc cgacaccgcc gagcgcatgc aggaggagca cgactcgctg gccgcgcgcc
     4021 tgcgcggcga ggtctccgag cacgagcgcc ggcgcagcga gctgtcgcgc ctgcactcgc
     4081 gcgaggcgag cgtcgcggcc cagctcgggg cgcgcgccga gggg
//
LOCUS       DASWNJ010000550         3728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_257959, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000550 DASWNJ010000000
VERSION     DASWNJ010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257959"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VGB85_09280"
     CDS             <1..631
                     /locus_tag="VGB85_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134261.1"
                     /translation="ASSVHYLGGSQGGIMGATVVAMAPNVPRGILVVGGGNYSLMIWR
                     SSAFSMLSDAWAGSQPDPQEREFLFALVQSAFDRADPLVFSELIDAPLAGGDPKRLLL
                     IESIGDAQVPNIATEVMARSYGMPMLAPGLTPVWGVEDANEPLLSGSALLQVDTGLGP
                     LPPKQNLPPADDNGAHGAAVDDPALIAVIERFIFKAIAENLCDGPCDPG"
     gene            946..2469
                     /locus_tag="VGB85_09285"
     CDS             946..2469
                     /locus_tag="VGB85_09285"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0134262.1"
                     /translation="MTDRTPGIEVRWLRWEDASEAILELRRRVYVEEQGFGMDVVASP
                     RDPTALHLGALVDGTLVAAITAYLYEPGATDLAQFELPALDGLTMQVGKRMELAAHRG
                     NGITEVLLAGLLRYTYECLRPARMFLVLRHVHRGLKNQYARMFDVELHAEIGAGDDSM
                     VVMKIEGEARLRRLYLKMRAMAESALRRCPVRVPSLVQYLAVEGRDALLSTARETAEN
                     LYVAPLSLADELPRLSAQNRLLFAEQRPRLAATFPTAIPAPTPAAIPATTPAAIPATT
                     PAATEASRPRLVDIGTGTGVYLSMVAREPALVNHLIKGIEPSPQLLAYARFAYPDIAF
                     AEGSAYATGEADGSVDVVTANFVFIHLRNPDLALLELWRIVKPGGLLYVVDVNDSTFT
                     GPTEIRSMIEAHHRHHEGDRTILATLPRRAAEFGFEPVAHHRTVVRNTGGVEPSFATN
                     EVRLGRMGLWGMLAFMGQREEIDEAFQVAQEHYLGTDCEISLDVETQVFRKPIVHRG"
     gene            2663..>3728
                     /locus_tag="VGB85_09290"
     CDS             2663..>3728
                     /locus_tag="VGB85_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134263.1"
                     /translation="MRAIESSSDGGFSGVRGRDALEEATEILEEPSKNIKRKILISLN
                     LIIALVGTLHVVMQLVTVGHLPDPARDSVVLPLIVFINLGSAIYNLRALGDERRVNDR
                     LDVVTRWVSVVIIQLACLIIVHFNTNTATSILMDHTLSILTIFLTGVILGRWAALAWF
                     AVTMASLGIAVHNRGLEFEYALMTRAELAALRDQGAAAVNSRVEAAADERLVPLPLAL
                     FAAVSVVFTALTVAATYFEAGLIGRVTAAMPAAVKKIQIAAREKQRLAQENLRMGLEL
                     DVAQRLQAMILPRGAELQKHADLEIAAHMVPASEIGGDIYDVLPGPEGSTLLVIGDVT
                     DHGLASGVVMLMTQASLRTAL"
BASE COUNT          551 a         1184 c         1418 g          575 t
ORIGIN      
        1 cgccagctcg gtgcactacc tcggcggctc gcagggcggg atcatgggcg cgacggtggt
       61 ggcgatggcg cccaatgtgc cgcgcgggat cctggtcgtc ggcggcggca actattcgct
      121 gatgatctgg cggtcgagcg ccttctcgat gctgtccgac gcgtgggccg ggagccagcc
      181 cgacccgcag gagcgcgagt tcctgttcgc gctggtgcag tcggcgttcg accgcgccga
      241 cccgctggtg ttctccgagc tgatcgacgc gccgctggcc ggcggcgacc ccaagcgact
      301 gctgctgatc gagtcgatcg gcgacgcgca ggtgcccaac atcgccaccg aggtgatggc
      361 ccgcagctac ggcatgccga tgctggcgcc ggggctgaca ccggtgtggg gcgtcgagga
      421 cgcgaacgag ccgctgctgt cgggctcggc gctgctgcag gtcgacaccg ggctgggacc
      481 gctgccgccg aagcagaacc tgccgccggc cgacgacaac ggggcccacg gcgcggcggt
      541 cgacgacccg gcgctgatcg ccgtgatcga gcgcttcatc ttcaaggcga tcgcggagaa
      601 cctgtgcgat gggccgtgcg atccgggatg accggatgac gggatgacgg gatgacgggt
      661 tgatgggtgc cgctgcgacg atgtgacgcg gcggtggatc acgggcggcc gatgcgcgag
      721 cgatgttccg tgacctgtcc tttcggacac gtcgacctgg gcgggcacgg aggcgagcat
      781 tggagcgggc attagcgcgc gtgtgcgctg gagcagtcga cggcgcgggc gcgttgacac
      841 tcgtttgcgc ggcttgctag tgtctcgctg cgcgcgccag gttttgcggc gcgacacgct
      901 ctgcggcgtg acaggccggc tccacgggat gagagggatg cgacgttgac ggaccgaaca
      961 ccggggatcg aggtgcggtg gctgcggtgg gaagacgcca gcgaggcgat cctcgagctg
     1021 cggcggcgcg tgtacgtcga ggagcagggc ttcgggatgg acgtggtggc gagcccgcgc
     1081 gacccgacgg cgctgcacct cggggcgctg gtcgacggca cgctcgtcgc ggcgatcacg
     1141 gcgtacctct acgagcccgg ggcgacggac ctggcgcagt tcgagctgcc agcgctcgac
     1201 gggctgacga tgcaggtcgg caagcgcatg gagctggcgg cccaccgcgg caacggcatc
     1261 accgaggtgc tgctcgccgg gctgctgcgc tacacctacg agtgcctgcg cccggcgcgg
     1321 atgttcctgg tcctgcgtca cgtgcatcgt ggcctcaaga accagtacgc gcgcatgttc
     1381 gacgtcgagc tccacgccga gatcggggcc ggcgacgact cgatggtcgt gatgaagatc
     1441 gaaggcgagg cccgtctgcg tcgcctgtat ctaaagatgc gtgcgatggc cgagagcgcg
     1501 ctgcggcgct gcccggtgcg cgtgccctcg ctggtccagt atctcgcggt cgagggccgc
     1561 gacgcgctgc tgtcgaccgc gcgcgagacg gcagagaacc tgtacgtcgc gccgctgtcg
     1621 ctggccgacg agctgccgcg actgtcggcg cagaaccgac tgttgttcgc ggagcagcgg
     1681 ccgcgcctgg cggcgacctt ccctaccgcc atcccggcgc ccacgccggc tgctatcccg
     1741 gcgaccacgc cggccgctat cccggcgacc acgccggcgg ccacggaggc gagccggccg
     1801 cggctggtcg acatcggcac gggcacgggc gtgtacctgt cgatggtggc gcgcgagcca
     1861 gcgctggtga atcaccttat taaagggatc gagccgtcgc cgcagctgct ggcgtacgcg
     1921 cgtttcgctt acccggacat cgcgttcgcg gagggcagcg cctacgcgac cggcgaggcg
     1981 gacggaagcg tcgacgtcgt caccgcgaac ttcgtgttca tccacctgcg caaccccgac
     2041 ctggcgctgc tcgagctgtg gcggatcgtg aagccgggcg ggctgttgta tgtcgtcgac
     2101 gtcaacgact cgacctttac cgggccgacc gagatccggt cgatgatcga ggcgcaccat
     2161 cgccaccacg agggcgatcg cacgatcctc gccacgttgc cgcgacgcgc cgcggaattc
     2221 ggcttcgaac cagtggccca ccatcgcacg gtggtccgca acaccggcgg cgtcgagccg
     2281 agcttcgcca ccaacgaggt ccgcctcggc cgcatgggcc tgtggggcat gctggcgttc
     2341 atggggcagc gcgaggagat cgacgaggcg tttcaggtgg cgcaggagca ctacctgggc
     2401 accgactgcg agatcagcct cgatgtcgag acgcaggtgt ttcgcaagcc gatcgttcat
     2461 cgtggctgaa agtggtcggg cccgcgatcg tggggcccgc agggcccgcg agcacggcag
     2521 gtccgagctt gcggagggcc acgccgggcc gggagcagga tgggcgccca cgacgcgggc
     2581 tgggcgcccg gagggcggcg acggggcatc gtgaggggag cagacccgat acaatgccgg
     2641 cgatgaggag cgagaggcgc tgatgcgggc gatcgagtcg agcagcgacg gcggcttttc
     2701 gggcgtgcgt ggccgggacg cgctcgagga ggcgacggag atcctcgagg agccctcgaa
     2761 gaacatcaag cgcaagatcc tgatctcgct caacctgatc atcgcgctgg tcggcacgct
     2821 gcacgtcgtg atgcagctgg tgacggtcgg ccacctgccg gatcccgcgc gcgacagcgt
     2881 ggtgttgccg ctgatcgtgt ttatcaacct cggctcggcg atctacaact tgcgggcgct
     2941 gggggacgag cggcgggtca acgaccggct cgatgtggtg acgcgctggg tgtcggtcgt
     3001 catcatccag ctcgcgtgcc tgatcatcgt ccattttaat acgaacacgg cgacgtccat
     3061 cctcatggac cacacgctga gcatcctgac gatcttcctg acgggggtga tcctcgggcg
     3121 ctgggcggcg ctggcgtggt tcgcggtgac gatggcgagc ctggggatcg cggtgcataa
     3181 ccgcgggctc gagttcgagt atgcgctgat gacccgggcg gagctcgcgg cgctgcggga
     3241 ccagggggcg gcggccgtga acagtcgggt cgaggccgcc gcggacgagc ggctggtgcc
     3301 gctgccgctg gcgctgttcg ccgcggtgtc ggtggtgttc acggcgctga cggtcgcggc
     3361 gacctacttc gaggcgggcc tgatcggtcg ggtgacggcg gcgatgccgg ccgcggtgaa
     3421 gaagatccag atcgccgcgc gcgagaagca gcggctcgcg caggagaacc tgcgcatggg
     3481 gctcgagctc gacgtcgcgc agcggctgca ggcgatgatc cttccgcgcg gggcggagct
     3541 gcaaaagcac gcggacctcg agatcgcggc gcacatggtg ccggcgtcgg agatcggcgg
     3601 ggacatttat gacgtgctcc cggggccgga gggctcgacc ctgctggtga tcggcgacgt
     3661 caccgatcac gggctggcct cgggcgtggt gatgttgatg acgcaggcgt cgctgcgcac
     3721 ggcgctca
//
LOCUS       DASWNJ010000551         1697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_186752, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000551 DASWNJ010000000
VERSION     DASWNJ010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186752"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VGB85_09295"
     CDS             complement(<1..345)
                     /locus_tag="VGB85_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134264.1"
                     /translation="MSRPRPRRLAATLVLGLAFSQFACAHGTQQLVKGGVEGGLEGTL
                     EALNDPHNKKLLKQLLQDADIKDAAHDLTEAITGGALDGMTEPERQAKLRDATDKFIR
                     TVADSVGKALNED"
     gene            complement(465..1361)
                     /gene="prmC"
                     /locus_tag="VGB85_09300"
     CDS             complement(465..1361)
                     /gene="prmC"
                     /locus_tag="VGB85_09300"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY0134265.1"
                     /translation="MPADAPPRSTRAQLWTVSELLQWTCDRFTREGLDDPRADALHLL
                     AQALGCERMQIYLRFDQHVDEPARATLRDLIRRRLTREPVAYIAGRRGFHALGLDLGV
                     DRRVLIPRPETEHLVDWLLEDLPRAPSPVHVLDVGTGSGAIALAIKHARRDVLVTACD
                     RSDDALAVARDNATRLGLDITLHRSDLLGALERPEFGWDAIAANLPYIPTGDLTGLQP
                     EVLRFEPRLALDGGPDGLDLVRRLVDTSPDMLAPGGKLLLEIGIGQAEATRAVLARRG
                     FVDIATRRDLGGIERVVAGTWR"
     gene            complement(1430..>1697)
                     /locus_tag="VGB85_09305"
     CDS             complement(1430..>1697)
                     /locus_tag="VGB85_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134266.1"
                     /translation="EDPRPHVQLRNFGPSGLELNLCVWIQSPRFRDDVIDNMNNRIYK
                     ALVAAKIEIPYSRHDLFLKDLPALQALLAAQQAPGASRTDDSPA"
BASE COUNT          228 a          497 c          720 g          252 t
ORIGIN      
        1 gtcctcgttg agcgccttgc cgaccgagtc ggcgacggtg cggatgaact tgtcggtcgc
       61 gtcgcgcagc ttggcctggc gctcgggctc ggtcatgcca tcgagggcgc cgccggtgat
      121 cgcctcggtc aggtcgtgcg cggcgtcctt gatgtccgcg tcctgcaaca gctgcttgag
      181 cagcttcttg ttgtgggggt cgttgagggc ctcgagggtg ccctccagac cgccctcgac
      241 gccccccttg acgagctgct gggtgccgtg ggcgcaggca aattgagaga acgcgagccc
      301 gaggacgagc gtcgccgcga gtcggcgtgg acgtgggcgg gacatgccag gccagcataa
      361 accgaggtgc aatgatgggc gattcggacg gtctcgcgag ggcgtgcccc gggcgtcgtc
      421 atgaatgccg gatcacggcg aatcgaggcg gatccgggct ggatctaccg ccatgtgccg
      481 gcgacgacac gctcgatgcc gccgaggtcg cgccgggtcg cgatgtcgac gaagccgcgg
      541 cgtgcgagga cagcgcgggt cgcctcggcc tggccgatgc cgatctcgag gaggagcttg
      601 ccgcctggag cgagcatgtc ggggctggta tcgacgaggc ggcgcacgag gtcgaggccg
      661 tcggggccgc cgtcgagggc gaggcgaggc tcgaagcgga gcacctcggg ttgcaggccc
      721 gtgagatccc cggtggggat gtaggggagg ttggccgcga tcgcgtccca gccgaattcg
      781 gggcgctcga gggcgccgag caggtcggag cggtgcaggg tgatgtcgag gccgaggcgg
      841 gtggcgttgt cgcgggcgac ggcgagggcg tcgtcggagc ggtcgcaggc ggtgacgagc
      901 acgtcgcggc gggcgtgctt gatcgccagg gcgatcgcgc cggagccggt gccgacgtcg
      961 agcacgtgca cggggctcgg cgcgcggggc aggtcctcga gcagccagtc gaccaggtgc
     1021 tcggtctccg ggcgcgggat gaggacgcgg cggtcgacgc cgaggtcgag gccgagcgcg
     1081 tggaagcccc ggcgaccggc gatgtaggcg accggctcgc gggtgaggcg gcggcggatc
     1141 aggtcacgca gggtggcgcg ggccggctcg tcgacgtgct ggtcgaagcg gaggtagatc
     1201 tgcatgcgct cgcagccgag ggcctgggcg agcaggtgga gggcgtcggc gcgggggtcg
     1261 tcgaggccct cgcgggtgaa gcggtcgcag gtccactgca gcagctcgga gacggtccag
     1321 agctgcgcgc gcgtggaccg gggcggtgcg tcggcgggca tgagcgcggc ggagtgtagc
     1381 gtgtccgccc gcgaggtccg ggccgccagc gcgtgaggtc tggcgcggct caggccgggg
     1441 agtcgtcggt gcgggaggcg ccaggagcct gttgcgcggc gagcagggcc tgcaaggcgg
     1501 gcaggtcctt gaggaacagg tcgtgtctcg agtacgggat ctcgatcttc gcggcgacga
     1561 gggccttgta gatccggttg ttcatgttgt cgatgacgtc gtccctgaag cgtgggctct
     1621 ggatccacac gcacaggttg agctcgaggc ccgaggggcc gaagttacgc agctgcacgt
     1681 gcgggcgcgg gtcctcg
//
LOCUS       DASWNJ010000552         3928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_645455, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000552 DASWNJ010000000
VERSION     DASWNJ010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645455"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VGB85_09310"
     CDS             <1..624
                     /locus_tag="VGB85_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134267.1"
                     /translation="GEPVTSTTGEPGTTTTTSTTSTTGSSSDTGTSTGEPPSEACVSD
                     DDCALHNDCCECYGLPKGQVDPSCPADCEQPICDMLQIDQAVCRFGVCITEKLDCDGS
                     KVVCKSLPPECPDGQIPGVLNNCWSGTCVPIVNCNAVPTCDLCPDGTMCVQKISKQQT
                     WPACEPIPAECGGEIDCECAGAAVCTGAFNICNDLEGNELSCGCPAC"
     gene            complement(634..1230)
                     /locus_tag="VGB85_09315"
     CDS             complement(634..1230)
                     /locus_tag="VGB85_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HEY0134268.1"
                     /translation="MSDRRTSPAARRNLGAITEVLAEVLPPRGLVLEIASGSGEHAVG
                     LAAKFPGVTWQPSDADPEALASIDAWAAEGPSNVRAAVELDARRWPWPVREAEAIVCI
                     NMIHIAPWAACLGLMRGAGALLAAGSPLFIYGAMKIGGEFTAPSNADFDASLRARDPA
                     WGVRDLEAVAAAGRDHGLELEKTLAMPANNFSLIFRRL"
     gene            1393..2262
                     /locus_tag="VGB85_09320"
     CDS             1393..2262
                     /locus_tag="VGB85_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134269.1"
                     /translation="MSHTAKLLAALMVTLAGALAGCLLDLPAVCGDGELDVEAGEECD
                     PAIVGEGQPPCDANCKQMTPASCGDGKLDLGEQCDGNDFGNKDCPSGKGFLSCTADCQ
                     LDESTCDHCGNGRVDVEVGEECDPKAPMFLLQPKNCSELTGPFKPYTSGQVNACIPDE
                     CKWFRGPCGYCGDDEVDPPHIVDLNFPEQQSVREVCDGLAFQPSALKDYCRARCPGEE
                     PLCAFDCADGCEQFMAPPADPKCCAQTNAPCPSEGDPLPCCAAYDLGLADLYSQEACT
                     DNFSGGLLSRVCK"
     gene            2306..3355
                     /locus_tag="VGB85_09325"
     CDS             2306..3355
                     /locus_tag="VGB85_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0134270.1"
                     /translation="MTTSATPSSARSSLVTGGCGFLGRAVVDALVARGDRVTVLDHRI
                     DRWRSDVQFVSADICDPRAVQDAIAGHDTVFHSASLVHTKHNRLEMVRAVNIDGTRNI
                     LRACQQSGVPRLVYVSSASVVYDGHDIKNGDESLPYAPSFPAPYAETKRIAEEEVLQA
                     NGHQGTLTCAIRPHVVFGPGDTRLVPAILARAREGKLKFRVGLGEKKLSDFTYVTNVV
                     DGMLAADERLTPGSPVAGQAYFITNGEPMSFWDFVAQVLPPLGFTPPRLAIPRSIAYS
                     AAAVREAYDTYVRGGTLNAEEGLSRFAIRYITSHHYFSHAKATRDLGYVPRVRMPEGI
                     QKAIAYCRELGAAKR"
     gene            3415..>3928
                     /locus_tag="VGB85_09330"
     CDS             3415..>3928
                     /locus_tag="VGB85_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0134271.1"
                     /translation="MADNSDEPGLRRFLGVFRYSRRALELVWSTSHTLTILFAVLTLI
                     AGILPAGVAYLGQLIVDAVVTASRQAGSEAAPVLRLVLAEAVLVIILLGVQRGISVVQ
                     ALLRAQLGHRVNVMILEKALTLDLTHFEDAEFYDKLTRARREASSRPLSLVGRTFSLV
                     QHGISLISFAA"
BASE COUNT          589 a         1495 c         1249 g          595 t
ORIGIN      
        1 ggcgagccgg tcacgagcac gaccggcgag cccggcacca ccacgaccac gagcaccacg
       61 agcaccaccg gcagctcgag cgacacgggc acgagcaccg gcgagccccc gtccgaggcc
      121 tgcgtcagcg acgacgactg cgcgctgcac aacgactgct gcgagtgcta cggcctgccg
      181 aagggtcagg tcgacccgag ctgccctgcc gactgcgagc agccgatctg cgacatgctg
      241 cagatcgatc aggctgtgtg ccgcttcggt gtgtgcatca ccgagaagct cgactgcgac
      301 ggcagcaagg tcgtctgcaa gtcgttgccg cccgagtgcc cggacggcca gatcccgggt
      361 gtgctcaaca actgctggtc cggcacctgc gtcccgatcg tcaactgcaa cgccgtgccg
      421 acctgcgacc tgtgccccga cggcaccatg tgcgtgcaga agatctcgaa gcagcagacc
      481 tggcccgcct gcgagccgat ccccgccgag tgcggcggcg agatcgactg cgagtgcgcc
      541 ggcgctgccg tgtgcaccgg cgccttcaac atctgcaacg acctcgaggg caacgagctc
      601 agctgcggct gcccggcctg ctgagccgtt tttttaaagc ctgcgaaaga tcagcgagaa
      661 gttgttggcc ggcatcgcca gcgtcttctc gagctcgagg ccgtggtccc gccccgcggc
      721 ggcgacggcc tcgagatctc gcacacccca ggcggggtcg cgcgcccgca gcgaggcgtc
      781 gaagtccgcg ttgctcggcg ccgtgaattc gccgcctatt ttcatcgcgc catagatgaa
      841 aagcggcgag ccggccgcga gcaacgcccc tgccccgcgc atcagcccca ggcacgccgc
      901 ccacggcgcg atgtggatca tgttgatgca cacgatcgct tccgcctcgc gcaccggcca
      961 cggccaccgc ctcgcgtcga gctccactgc cgcccgaaca ttgctcgggc cctcggccgc
     1021 ccacgcgtcg atgctggcca gcgcctccgg gtccgcgtcg ctcggctgcc aggtcacccc
     1081 cggaaacttc gctgcgaggc ccaccgcgtg ctccccggac ccacttgcga tttcgagcac
     1141 gagccctcgc ggcggcagca cctccgcgag cacctcggta atcgccccga ggttacgccg
     1201 tgccgccggt gacgtccgcc tgtcgctcat gccgggcatg gtaccggccg gtgttgcagt
     1261 tgtcgcgttc gtcaactgtc gcagtttcgt caggtttcgt ttcgccggtg cacggctcac
     1321 gacttgtcac ttgtggccgg cttgcggccg gtctcgcgcc gcggtgatat agtggcgacg
     1381 atggcacggt cgatgagcca caccgcgaag ctgctcgcgg cgctgatggt gacgctcgcg
     1441 ggggcgctcg ccggctgtct gctcgacctc ccggcggtgt gcggcgacgg cgagctcgat
     1501 gtcgaggccg gcgaggagtg tgaccctgcg atcgtcggcg agggacagcc gccgtgcgac
     1561 gccaactgca agcagatgac cccggcgagc tgcggcgacg gcaagctcga cctcggcgag
     1621 cagtgcgacg gcaacgactt cggcaacaag gactgcccga gcggcaaggg cttcctcagc
     1681 tgcaccgccg actgccagct cgacgagagc acctgtgacc actgcggcaa cggccgcgtc
     1741 gacgtcgagg tcggcgagga gtgtgacccc aaggccccga tgttcctgct gcagcccaag
     1801 aactgctccg agctgacggg gccgttcaag ccgtacacct cggggcaggt caacgcctgc
     1861 atccccgacg agtgcaagtg gttccgcggg ccctgcggct actgcggcga cgacgaggtc
     1921 gacccgccgc acatcgtcga cctcaacttc cccgagcagc agtcggtccg cgaggtctgc
     1981 gacggcctcg cgttccagcc gagcgcgctc aaggactact gccgcgcgcg ctgccccggc
     2041 gaggagccgc tgtgcgcgtt cgactgcgcc gacggctgcg aacaattcat ggccccaccg
     2101 gccgacccga agtgctgcgc gcaaaccaac gccccctgcc cgagcgaagg cgaccctctc
     2161 ccctgctgcg ccgcctacga cctcggcctc gcggacctct actcacagga ggcctgcacc
     2221 gataatttct cgggcgggct gctcagccgc gtgtgcaagt agccggtggc gagcggctcg
     2281 gcccctggtc tatgctccgc gccccgtgac cacctccgcc actccctcgt ccgcccgctc
     2341 gtccctcgtc actggcggct gcggcttcct cggccgcgcc gtcgtcgacg cgctcgtcgc
     2401 tcgtggtgac cgcgtcaccg tgctcgacca ccgcatcgac cgctggcgca gcgacgtgca
     2461 attcgtgtcc gccgacatct gcgacccccg ggccgtgcag gacgcgatcg ccggccacga
     2521 caccgtgttt catagcgcct cgctcgtgca caccaagcac aatcgcctgg aaatggtgcg
     2581 cgccgtcaac atcgacggca cccgcaacat ccttcgcgcc tgccagcagt ccggcgtgcc
     2641 gcggctcgtg tacgtcagct cggcgagcgt cgtctacgac ggccacgaca tcaagaacgg
     2701 cgacgagtcg ctgccctacg cgccctcgtt cccggccccc tacgccgaga ccaagcgcat
     2761 cgccgaggag gaggtcttgc aggccaacgg ccaccaggga accctgacct gcgcgatccg
     2821 tccgcacgtc gtcttcggcc cgggcgacac ccgcctcgtc ccggcgatcc tcgcccgcgc
     2881 gcgcgagggc aagctcaagt tccgcgtcgg cctcggcgag aagaagctgt ccgacttcac
     2941 ctacgtcacc aacgtcgtcg acggcatgct cgccgccgac gagcgcctca cgcccggctc
     3001 gcccgtcgcg ggtcaggcct acttcatcac caacggcgag ccgatgtcgt tctgggactt
     3061 cgtcgcgcag gtgctgccgc cgctgggctt cacgccgccc cgcctggcga tcccccgcag
     3121 catcgcctac agcgccgccg cggtgcgcga ggcctacgac acctacgtcc gcggcggcac
     3181 gctcaacgcc gaggagggcc tgtcgcgctt cgcgatccgc tacatcacca gccaccacta
     3241 cttcagccac gccaaggcga cccgcgacct cggctacgtg ccgcgcgtgc gcatgcccga
     3301 gggaattcag aaggcgatcg cctactgccg cgagctgggc gccgcgaagc gctagcgcgt
     3361 cgaccgccag cgtccccctg tcagatggcg catccgacca ggcaccatgt cggcgtggcc
     3421 gacaattcgg acgagcccgg cctgcgccgc ttcctcggcg tgttccgcta cagccggcgc
     3481 gcgctcgagc tggtgtggtc gacgagtcac acgctgacga tcctgttcgc ggtcctgacg
     3541 ctgatcgccg gcatcttgcc ggccggcgtc gcctacctcg gacagctgat cgtcgacgcc
     3601 gtcgtgaccg cctcgcggca ggccggctcg gaggccgcgc cggtgctgcg cctggtgctc
     3661 gccgaggccg tgctcgtcat catcctgctc ggggttcagc gcggcatcag cgtcgtgcag
     3721 gcgctgctgc gcgcccagct cggccaccgc gtcaacgtca tgatcctcga gaaggcgctg
     3781 acgctcgacc tgacgcactt cgaggacgcc gagttctacg acaagctgac ccgcgcccgc
     3841 cgcgaggcct cgagccgccc gctgagcctc gtcggccgca ccttctcgct ggtccagcac
     3901 ggcatctcgc tgatcagctt cgccgcct
//
LOCUS       DASWNJ010000553         5392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_645464, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000553 DASWNJ010000000
VERSION     DASWNJ010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645464"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1820)
                     /locus_tag="VGB85_09335"
     CDS             complement(<1..1820)
                     /locus_tag="VGB85_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134272.1"
                     /translation="MASESGAVVRAGLVIAGAGLFLSLPAIVLGRLERLGREVQAADP
                     KTAAELLQSLPSRPVVRYFAPFAWRTLQDGILQLKVGDGRAAAANFAETARLVHQPEA
                     VMLISAQAHALVLAGERTEAYTLLQRLAKENLLGSRDQLDLGIVLLIESQKKNKQALA
                     YIEAARKTIGDHPRVLAALALALQKTERIDEASELLEQVQISLQGGEADPIVEDLVKR
                     ARKGLQDFIEAQLRRERRSRSRRTTIVVSSDLAASEIVSGEIGAGDHQDAAQLDNPTT
                     SFRPAETGAPSVISSKSVDGQLTNEERTMLNLPNSPTRRAADARASAFSIEEDGPGDV
                     EEVEEEAAPAKPEPSKPPPKPAAKAEPVAASPIAASPVAASPEPAARPAAVEPAKPAA
                     PSEPAAPEATASDRPARPGTGVGGGKAPTEKHPTLKAAASKPEPPKPATASKPEPVSS
                     KPPVAATATPPKPAPVTPPVARAEAVPTPTIAESSGPFTSSLFDSLFNEPTAPVAPEN
                     ISLINPAVAVPSPPPSDLSKILPPLAPPLPPEPVPSKPAPKSADEVDVPVFRRKPAAP
                     PNQRGTDRSTQLGNLPTGGNSGSPLSSLPVAGGAAMPTRTP"
     gene            2077..3066
                     /locus_tag="VGB85_09340"
     CDS             2077..3066
                     /locus_tag="VGB85_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134273.1"
                     /translation="MTSPLDLGRFVQRRQFRSFEQDLARVTYAYGVDVRRFRWLNQSM
                     AAQTVMRALELRWSAVAEELMRGELQVPEGAATLPFLQEIVRIAELLRVTLPSVRLVT
                     PSARGRWPLVTALGNARNSDDWLVLDAHALAELSSPQRAFLLAQGLGHIQCGHAALFM
                     AHYVSFHDHRGHAGIRLALRPFSRLAAFSADRAGLLASGEFEPAAQMLVRSVEHLPPW
                     YPPLADLSDRHRALEEFDRSRVVARVRSSRRFAEEHPSVRALVDQLMQHSEPAALTDG
                     AATAPEITSASTADTRAAAAAGPQAFAAGYEVPAEAWSLARCDQRLTQRLGIF"
     gene            3066..4940
                     /locus_tag="VGB85_09345"
     CDS             3066..4940
                     /locus_tag="VGB85_09345"
                     /inference="COORDINATES: protein motif:HMM:NF012570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEY0134274.1"
                     /translation="MSNADLSTASDPAVLATTLRDRARELADIAGQLGLPGLGEHIVT
                     DTTRRLEHAQLRVLVIGEIKQGKSTLINALLGEALLPSGVTPTTGAVVQIVRDDQLGR
                     FLVSPDGLRSELPPERFAKLAKGKEEFLGTLLVTTTSEHLPPGVELIDTPGINDLERF
                     RSLMSRGELPRGDAIILVLDATQVLKLTEVAFLRDALVALGGTEGTGATLLLALNRID
                     LVPDAERGTLIDHIHRELTAVLPGPVEVFQTDARGASKQPDGEGIGTREVRRLRARLQ
                     TLAGRSTTVLPTRARASLQRSVDLLLHHTSIEARALRLESAALQAELKEVRETLVQQR
                     LDLDKIERTIAEGRTKIAQSCHQHLIDFRNELQTSILSQLERNDLRSTADLLPGAIQD
                     ALLHFTYHETERLRLELEQLSRDAIHTCGEQAQRRLAETMLNLGFRGPVVQLQPPSVG
                     VEAGTLAVGIAGTAIMYFGNIVAGMLVTIAAPLATMFLRERSIRELREAARKSVPHAL
                     SQSFSELEGTLLRAVDGHLAGLREYIVLAHNQLGAHLQALLERAAAVHSTAALPAADP
                     AATASTPAANLTEKSAAPDPRRSALVELRALEQRLEHMRGELAKLADEPAAGPATPKT
                     "
     gene            5007..>5392
                     /locus_tag="VGB85_09350"
     CDS             5007..>5392
                     /locus_tag="VGB85_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134275.1"
                     /translation="MTNGSDDRNDPANTLRSVGSTLGGWAKRVGKAITEATGPQHPEE
                     VVRALGQAHDLRRNGQYAAAYERLQTQLKARPGDPSLLLSLALTIVEQALVVPDRADV
                     LHLFAEGLGKRSERNPAGPLVEATLHL"
BASE COUNT          735 a         2137 c         1750 g          770 t
ORIGIN      
        1 ggcgtgcggg tgggcatcgc agcaccaccg gcgaccggca acgacgacag cggcgagccc
       61 gagtttccgc ccgtgggcag gttgccgagc tgcgtcgacc ggtctgtccc gcgctgattc
      121 ggcggcgccg ccggcttgcg acggaacacc ggcacatcga cctcgtccgc cgacttcggc
      181 gccggcttgc tcggcacggg ctcgggcggc agcggcgggg ccaacggtgg caggatcttg
      241 ctgaggtcgc tcggtggcgg cgagggcacg gccacggcgg gattgatcag cgagatgttc
      301 tccggggcga ccggcgccgt cggctcgttg aacagcgagt cgaacagtga gctggtgaac
      361 ggcccgctgc tctcggcgat cgtcggagtc ggcactgcct cggcgcgggc caccggcggg
      421 gtcacgggcg caggcttcgg cggcgttgca gtcgcggcga ccggcggctt gctgctcacg
      481 ggctccggct tgctcgcggt cgcgggcttc ggaggctcgg gcttgctcgc ggccgccttc
      541 aaggtcgggt gcttctcggt cggcgccttc cctcccccca cgcccgtccc gggccgcgca
      601 ggacgatcgc tcgccgtcgc ctcgggcgct gcaggctcgc tcggcgccgc aggcttcgcc
      661 ggctcgacgg cagccggcct cgccgcgggc tcggggctgg cagcgaccgg gctggcagcg
      721 atggggctgg cagcgacggg ctcggccttg gctgcgggct tgggcggggg cttgctcggc
      781 tccggcttcg cgggtgcggc ctcttcctcg acctcctcga cgtcacccgg cccgtcctcc
      841 tcgatcgaga aggcgctcgc gcgggcgtcc gcggcccggc gcgtcggcga gttcgggagg
      901 ttgagcatcg tccgctcttc gttcgtcagc tggccgtcga ccgacttgct cgagatgaca
      961 ctcggggctc cggtctcggc cggacggaag ctggtcgtcg ggttgtcgag ctgcgcggca
     1021 tcctggtgat cgccggcgcc gatctctccc gacacgatct cgctcgccgc gaggtcgctc
     1081 gacacgacga tcgtcgtgcg ccgcgatcgc gagcgacgct cgcgtcgcag ctgggcctcg
     1141 atgaagtctt gcaggccctt gcgcgcgcgc ttgaccaggt cctcgacgat cgggtcggcc
     1201 tcgccgcctt gcaggctgat ctggacctgc tcgagcagct ccgacgcctc gtcgatgcgc
     1261 tcggtctttt gcagcgcgag ggccagcgcc gcgagcacgc gcgggtggtc gccgatcgtc
     1321 ttgcgggcgg cctcgatata cgccagcgcc tgcttgttct tcttctgcga ctcgatcagc
     1381 agcacgatgc cgaggtcgag ctggtcgcgc gagccgagca ggttctcctt ggcgagtcgt
     1441 tgcagcagcg tgtacgcctc ggtccgctct cccgcgagca ccagagcgtg cgcctgggcg
     1501 ctgatcagca tgacggcctc gggctgatgg accaggcgcg ccgtctcggc gaagttggcc
     1561 gccgcggccc ggccgtcgcc gaccttgagc tgaaggatgc cgtcttgcag cgtgcgccag
     1621 gcgaagggcg cgaagtagcg gaccaccggc cggctgggca ggctctgcag cagctccgcc
     1681 gcggtcttcg ggtcggcggc ctggacctcg cgaccgaggc gctccagccg cccgagcacg
     1741 atcgccggca acgacaggaa cagccccgcg ccggcgatca ccaggccggc ccgcacgacg
     1801 gcgccggact cggacgccat gagcgcgacg aggatgagcg ccgccggtat cgcgatctgc
     1861 gcaatgcgtc cctgcaccgg tcggacgctg cgcatccaca ggtgcggcgg aaccgtgacg
     1921 gccgggagct caatgtgctt ggcagcagcc atgggaccgt tcgttgccgg gcatacctag
     1981 atcagcggtg agccgcaagc tggttcgcgt gcccctcggc acctttttgg atcttcgcgg
     2041 cgacagatcc ggcagctgcc tgctaaaatc actccagtga cctccccact cgatctcggt
     2101 cgcttcgtac agcgccggca gtttcgctcc ttcgagcagg acctggcccg tgtcacctac
     2161 gcctacggcg tcgatgtccg gcgctttcgc tggctcaacc agtcgatggc ggcgcagacc
     2221 gtcatgcgcg cgctcgagct gcgctggagc gccgtcgcgg aggagcttat gcgaggggag
     2281 ttgcaggtcc ccgaaggtgc cgccacgctg ccatttctgc aagagatcgt ccgaatcgcg
     2341 gagctcctgc gcgtcaccct cccctccgtc cgcctcgtca ccccctccgc acgcggacgc
     2401 tggcccctcg tcaccgcgct cggcaacgct cgcaacagcg acgactggct cgtcctcgac
     2461 gcccacgccc tcgccgagct ctcttcgccg caacgcgcct tcctcctcgc gcagggcctc
     2521 ggccacatcc agtgcggtca cgcggccctc ttcatggccc actacgtctc gttccacgac
     2581 caccgcgggc acgccggcat ccgcctcgcg ctgcgcccgt ttagtcggct cgccgcgttc
     2641 tcggccgacc gcgccggcct gctcgcctcg ggcgaatttg agcccgccgc gcagatgctc
     2701 gtccgctcgg tcgagcatct gccgccgtgg tatccgccgc tcgcggacct gtccgaccgc
     2761 caccgcgcgc tcgaggagtt cgaccgcagc cgcgtcgtcg cccgcgtccg ctcgagccgg
     2821 cgcttcgccg aggagcaccc ctccgtgcgc gcgctcgtcg accagctgat gcagcacagc
     2881 gagccagctg cgctgaccga cggcgccgcc accgcgccgg agatcacgag cgcctcgacg
     2941 gcggacaccc gcgccgccgc agccgccggt ccacaggcgt tcgccgcggg ctacgaggtc
     3001 cccgccgaag cctggtcact cgcgcgctgc gaccagcgcc tcacccagcg tctggggatc
     3061 ttctgatgtc gaacgccgat ctttccaccg ccagcgatcc cgccgtcctc gccaccaccc
     3121 tccgcgaccg cgcccgcgag ctcgccgaca tcgccggcca gctcggcctg cccgggctcg
     3181 gcgagcacat cgtcaccgac accacccgcc gcctcgagca cgcccagctg cgcgtgctcg
     3241 tgatcggcga gatcaaacag ggcaagagca ccctgatcaa cgccctgctc ggcgaggccc
     3301 tgctgcccag cggcgtcacc ccgaccaccg gcgccgtcgt ccagatcgtc cgcgacgacc
     3361 agctcggccg cttcctcgtg tcgcccgacg gcctgcgcag cgagctcccg ccggagcgct
     3421 tcgccaagct cgccaagggc aaggaggagt tcctcggcac gttgctggtc accaccacca
     3481 gcgagcacct cccgcccggc gtcgagctga tcgacacccc cggcatcaac gacctcgagc
     3541 gcttccgcag cctgatgagc cgcggcgagc tcccgcgcgg cgacgcgatc atcctcgtgc
     3601 tcgacgccac ccaggtgctc aagctgaccg aggtcgcgtt cctgcgcgac gcgctcgtcg
     3661 ccctcggcgg caccgagggc accggcgcca ctctcctgct cgcgctcaac cgcatcgacc
     3721 tcgtccccga cgccgagcgc ggcaccctca tcgaccacat ccaccgcgag ctgaccgccg
     3781 tgctgcccgg ccccgtcgag gtgttccaga ccgacgcccg cggcgccagc aaacagcccg
     3841 acggcgaggg catcggcacc cgcgaggtcc gccgcctgcg tgcgcgtcta cagaccctcg
     3901 caggccgcag caccaccgtc ttgccgaccc gcgcccgcgc gagcctgcag cgcagcgtcg
     3961 atctcttgct gcatcacaca tcgatcgagg ccagggcgct gcgcctcgag tccgccgctt
     4021 tgcaggccga gctcaaggag gtccgcgaga ccctcgtgca gcagcgcctc gacctcgaca
     4081 agatcgagcg cacgatcgcc gagggccgca cgaagatcgc ccagagctgc caccagcacc
     4141 tgatcgattt ccgtaacgag ctccagacct cgatcctcag ccagctcgag cgcaatgacc
     4201 tgcgctcgac cgccgacctg ctgcccggcg cgatccagga cgccctgctg cacttcacct
     4261 accacgagac cgagcgcctg cgtctcgaac tcgagcagct cagccgcgac gcgatccaca
     4321 cctgcggcga gcaggcccag cgacgcctcg ccgagaccat gctcaacctc ggctttcgcg
     4381 ggccagtggt acagctgcaa ccaccgtccg tcggcgtcga ggccggcacc ctcgcggtcg
     4441 gcatcgccgg caccgcgatc atgtactttg gcaacatcgt cgccggcatg ctggtgacga
     4501 tcgccgcccc gctggcgacg atgttcctgc gcgagcgatc gatccgcgag ctgcgagagg
     4561 ccgcccgcaa gtccgtcccg cacgcgctca gccagagctt ctccgagctc gagggcaccc
     4621 tcctgcgcgc cgtcgacggc cacctcgccg gcctgcgcga gtacatcgtg ctcgcccaca
     4681 accagctcgg cgcccacctc caggccctgc tcgagcgcgc cgccgccgtg cactcgaccg
     4741 ccgcgctgcc cgccgccgac ccggccgcga ccgcgagcac gcccgccgcc aacctgaccg
     4801 aaaagtcagc cgccccggac ccgcgccgca gcgcgctcgt cgagctccgc gcgctcgagc
     4861 agcgcctcga gcacatgcgc ggcgagctcg ccaaactcgc cgacgagccc gccgccgggc
     4921 ccgccacccc caagacgtga gccagcccgc gcatcccggc agcgcacccg cgccctgacc
     4981 tccgctaccc tcccgcacgg cccctcatga ccaacggcag cgacgaccgc aacgatcccg
     5041 ccaacaccct gcgatcggtc ggcagcacgc tcggcggctg ggccaagcgc gtcggcaagg
     5101 cgatcaccga agccaccggg ccgcagcacc ccgaggaggt cgtgcgcgcg ctcggtcagg
     5161 cccacgacct gcgccgcaac ggccagtacg ccgccgccta cgagcgcctg cagacccagc
     5221 tcaaggcccg ccccggcgat ccgagcctgc tgctcagcct cgccctgacg atcgtcgagc
     5281 aggcgctcgt cgtgcccgac cgcgccgacg tcctgcacct gttcgccgag ggcctcggca
     5341 agcgcagcga gcgtaacccc gccggcccgc tcgtcgaggc cacgctgcac ct
//
LOCUS       DASWNJ010000554         5134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_80953, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000554 DASWNJ010000000
VERSION     DASWNJ010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80953"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VGB85_09355"
     CDS             complement(<1..842)
                     /locus_tag="VGB85_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134276.1"
                     /translation="MLMACCAGCPPDEGEATANDPATSDPSSGPGGPSSGDIDDPTGE
                     TGEGPTSTSQDTAPDSTTTATATTATDTTTTGEPGDGTSGDTTTDDATGGTTTGGATP
                     PGCMQREFTAGTLTMLDFTVKDAEYSKQLDRIVAVSHDPPALHLYDPWTGEAEGVELP
                     LAATSVSVGPGGLFAAVGHDGWVSYVDLVTRAVVATHPVTADVLDVVLADNGYIYAFP
                     RQDQWEQIYTIEIATGTQTQSGDWAIYAGTVAKLHPDGDALYGANNGLSPSDIEKYDI
                     QGGTA"
     gene            1334..2962
                     /locus_tag="VGB85_09360"
     CDS             1334..2962
                     /locus_tag="VGB85_09360"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY0134277.1"
                     /translation="MNPNLPVFTILSFVGGGIRGLMSATILQRLNDATGLFVQYTDLL
                     AGCSTGSIITSELLANTPVEKLIALFKGKEVKFYNKMHTDSSRPAYSIDEVLLSQRAL
                     HQDKKVADAGRNVLFVSFNVGGLEKQDDGLIVPKPWDTRMFTNMLNTEQDRRDDLDGN
                     ADTPIAVAATSSGAMPGQLGSLEGNVDGAFFNHDPTVAAIALAVRNGHPLENIVAITV
                     GTGLMPDWIASDTHDWGAKQWMKGAGNPFDNTPPFLMNQSGPSPVLDMCLNGTSAEMM
                     PRLAKMLLGDRYVNINPRLPCFIPENSTNPQALALLEHHGATVNIDDAVALIKKYWPD
                     AMVTQRAPAPRRASAAAPASAAAPASAAAAKRIADAPTATPGHGEFFYIQRHGSNDVL
                     DVSGGNIAAGTSVIAFPRRTTGLNQQWQFVPAEGEPGWWYLQTAMATKMVLTLQEPTQ
                     PIPQIVMEKIDPALKQRQLWNLLSTHKLGWWFIQSKSHSQRVEIDTTNPNAFTPTVIG
                     AAEEGDIKDAVGVALNYLASELMAWGFYAVSDEQ"
     gene            3313..>5134
                     /locus_tag="VGB85_09365"
     CDS             3313..>5134
                     /locus_tag="VGB85_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134278.1"
                     /translation="MVDSICKLLATPTRLATMASLLTLGCDAGPSARPPAAVQAPAPT
                     QREGELPEEPAEALVTTPVTPVQYLRQLAFDLRGGPPTVAELARVAEEGSVSQGLVDE
                     MLLSQSFLEQVRNWHAEMLWPNLQRYRLQAESVGALRSPSNDPKEIGSADYDSVGADP
                     LLVVDAEARAKYVVGIESEAVAGSLRGGAHAHNTHNCDFRDEAEYPDPSVVGTPQNEY
                     TVPAERSGTGQEYKAKYYSEDPASLGAVLPIRDHLHCANWCRSEACEPDQGFVDANAD
                     NAHDCYSFMDTPGDDPNGRHELDVPGMRCKDGFVRELNACNFWEVLGPDGWMHRAQVW
                     GPNGRLTSAQQQREGWRWTTQYWTKGEKIKMCAVEAQDREFGLYNKNPDGTPLACSKV
                     MATGNWWYLDPSCGCGPEGAYCQPSHIEYRTNAETRSEYRLRRALEEEPLQIIGSVVA
                     RDEDYLSILTTARSFVNGPLAFAFLHQADVLRGEGPLRISPPGPEDPVWSQVPFESDA
                     WVETTRPSHHSGILTTLGYLIRFPTYRARIAQYRRKFLCSAEFDYAPQPDPADHNPDI
                     AERSGCSNCHARLEFDGMYFGRYPDRTGLYLDPEKFPIVNK"
BASE COUNT          851 a         1748 c         1752 g          783 t
ORIGIN      
        1 gcggtgccgc cctgaatgtc gtacttctcg atgtccgagg gcgacaggcc gttgttggcg
       61 ccgtacaggg cgtcgccgtc cgggtgcagc ttggcgaccg tgccggcgta gatcgcccag
      121 tcgccgctct gcgtctgcgt cccggtggcg atctcgatcg tgtagatctg ctcccactgg
      181 tcctgccggg ggaacgcgta gatgtaacca ttgtcggcga gcacgacgtc gagcacatcg
      241 gccgtgaccg ggtgggtggc caccaccgcg cgcgtgacga ggtcgacgta cgagacccag
      301 ccgtcgtgcc cgacggcggc gaacaggccg cccgggccga ccgagacgct cgtcgccgcc
      361 agcggcagct cgacgccctc ggcctcgccc gtccacgggt cgtacaggtg cagcgcgggc
      421 ggatcgtggc tcaccgcgac gatgcggtcg agctgcttgc tgtactcggc gtccttgacc
      481 gtgaagtcga gcatcgtcag cgtcccggcc gtaaactcgc gctgcatgca gcccggtggg
      541 gtggcgccgc cggtggtcgt gccgccggtg gcatcgtcgg tggtcgtatc accggaggtg
      601 ccgtcgccgg gctcgccggt cgtcgtggtg tcggtcgccg tggtggcggt cgccgtggtc
      661 gtcgagtccg gggcggtgtc ctgcgaggtc gaggtcgggc cctcgccggt ctcgccggtc
      721 gggtcgtcga tgtcgccgct cgacggcccg cccgggccgc tcgacgggtc ggacgtggcg
      781 ggatcgttcg ccgtggcctc gccctcgtcg ggcggacagc cggcgcagca cgccatcagc
      841 acggccagcg cggccggaag tcgcgttcgc ggggcggggg aggggaggga tggatttgcg
      901 tgtacctgga agatccgcat gcaagctccg ggacgctgtg gtccgcatcg accacgtgtc
      961 cgtgctggcg tccatcgatc acagcgatgg ttgtttgcgg gtcgccgacg gcgagggccg
     1021 acgccacggg cgcgaccctg aacgacgagg gcgtggccat cggatgatcg atcccgtcgt
     1081 cgcatgggag gtagtgacgg aacaggcgag gtcgatgagc gatagtttcg atcgccctga
     1141 acgatcggta tgcgcgataa acagcgttaa accgcgccga ccgcgaggcg accatatttt
     1201 tgtgatcgca gacacacgtg tgcacgctcg catttgcagg cccctctgct ttttgcgatg
     1261 agtggcagga gggaatgaac gcctgtcttt cctccgccgg ctcggcgcat cgagcattcg
     1321 ccaggagaat tcaatgaacc cgaatctgcc cgtattcacc atcctatctt tcgtcggcgg
     1381 cggcatccgc gggctcatgt ccgcgacgat cttgcagcgc ctgaacgacg ccacggggtt
     1441 attcgtgcag tacaccgact tgctcgccgg ctgctcgacc ggctcgatca ttaccagcga
     1501 gctgctggcc aacacgccgg tggagaagct gatcgcgctg ttcaagggca aagaggtcaa
     1561 gttctacaac aagatgcaca ccgattcctc caggccggcg tactcgatcg acgaggtcct
     1621 cctttcccag cgggccctgc accaggacaa gaaggtggcc gacgccggac gcaacgtgct
     1681 cttcgtgtcg ttcaacgtcg gcggcctcga gaagcaagac gatgggctca tcgtgccgaa
     1741 gccgtgggac acgcgcatgt tcaccaacat gctcaacacg gagcaggacc ggcgcgatga
     1801 tctggacggg aacgccgaca cgccgatcgc cgtggccgcg acgtccagcg gcgcgatgcc
     1861 cggtcagctc ggttcgctgg agggcaacgt cgacggcgcc tttttcaacc acgatccgac
     1921 ggtcgccgcg atcgcgctgg ccgtgcgcaa cggccacccg ctcgagaaca tcgtggcgat
     1981 caccgtcgga accggcctca tgcccgactg gatcgccagc gatacgcacg actggggcgc
     2041 caaacagtgg atgaagggcg cgggcaaccc gttcgacaac acgccgccgt tcctcatgaa
     2101 ccagtcgggg ccctcgcccg tgctcgacat gtgcctaaac ggtacgtcag ccgagatgat
     2161 gccgcggctg gccaaaatgc tgctcggaga tcggtacgtg aacatcaacc cgcggctgcc
     2221 gtgcttcatt ccggagaact cgaccaaccc gcaggcgctc gcgctgctcg agcatcacgg
     2281 ggccacggtg aacatcgacg atgcggtcgc gctgatcaaa aaatactggc ccgacgctat
     2341 ggtcacccag cgcgcacctg ctcccaggag agccagcgcg gcagcccccg cgagcgcggc
     2401 agcccccgcg agcgcggcag cggccaagcg catcgcggac gccccgacgg ccacgccggg
     2461 tcacggcgag ttcttctaca tccagcgcca cggctccaac gacgtgctgg acgtgtccgg
     2521 ggggaatatt gcggcgggca ccagtgtgat cgcatttccg cgcagaacga ccggcctcaa
     2581 ccagcagtgg caattcgtcc ccgccgaagg cgagccgggc tggtggtatc tacagacagc
     2641 gatggcgacg aagatggtcc tgacgctgca agaacccacg cagccgatcc cgcagatcgt
     2701 catggagaag atcgacccgg ccctcaagca gcgccagctg tggaacctcc tatcgacaca
     2761 caagctcggc tggtggttca tccagagcaa gagccacagc cagagggtcg agatcgacac
     2821 caccaacccc aacgcgttca ccccgaccgt gatcggcgcc gccgaggagg gcgatatcaa
     2881 ggacgcggtc ggcgtcgcgc tgaactacct ggcgtccgag ctgatggcct ggggcttcta
     2941 tgcggtgagc gacgagcagt gacgtgagac gcggccgcga gcccgactgc cagctgctca
     3001 ggatgcagtc gtcgtcggtc ttgcaccggc catcgccggc gacctcttcc gtcggagcgc
     3061 gaacgacatc ggccggcggc gccagaccat ggtcgggcga ctatcaggtg catacggcgc
     3121 gtcggcgcgg cgagcgagct gatagcgcgc ctgcgtagca cggtcacccc ggcggcgccg
     3181 aggcctggcc gatgttgcgc atgcgggcgg cgacgtcctg cccgcggctg tcgcctgtga
     3241 agctccacgg cgggtttttc agggggcgct ggcccgcttc aggtcggcga tggcttcgac
     3301 tatgttgtcg gcgtggtgga ctcgatctgc aagctcctgg cgacgccgac ccggctcgcg
     3361 acgatggcgt ccctgctgac cctcggctgc gacgccggtc cgtcggcgcg gccgcccgcc
     3421 gccgtgcagg ccccggcgcc gacgcaacgc gagggtgagc tgcccgagga gcccgcagag
     3481 gcgctggtga cgacgccggt cacgccggta cagtatctgc gccagctggc cttcgacctg
     3541 cgcggcgggc cgccgaccgt cgcggagctc gcccgggtcg ccgaggaggg cagcgtctcg
     3601 cagggcctgg tcgacgagat gctgctgtcg cagagcttcc tcgagcaggt ccgcaactgg
     3661 cacgcggaga tgttgtggcc caacctgcag cgctatcgcc tgcaggcgga gtcggtcggg
     3721 gccctgcggt cgcccagcaa cgaccccaag gagatcggct cggccgacta cgacagcgtc
     3781 ggcgccgacc cgctgctcgt cgtcgacgcc gaggcgcgcg cgaagtacgt cgtcggcatc
     3841 gagagcgagg cggtcgccgg ctcgctgcgc gggggcgcgc acgcgcacaa cacccacaac
     3901 tgcgacttcc gcgacgaggc cgagtatccc gacccgtcgg tcgtcggcac gccgcagaac
     3961 gagtacacgg tgccggccga gcgcagcggc accgggcagg agtacaaggc caagtattac
     4021 agcgaggatc cggcgtcgct cggcgcggtg ttgccgatcc gcgatcacct gcactgcgcc
     4081 aactggtgtc gcagcgaggc gtgcgagccg gatcagggct tcgtcgacgc caacgcggac
     4141 aacgcccacg actgttacag cttcatggac acccccggcg acgacccgaa cggtcgccac
     4201 gagctcgacg tcccgggcat gcgctgcaag gacggctttg tccgcgagct caacgcctgc
     4261 aacttctggg aggtgctcgg cccggacggg tggatgcatc gcgcccaggt ctgggggccc
     4321 aacggccgcc tgaccagcgc tcagcagcag cgcgagggct ggcggtggac cacgcagtac
     4381 tggaccaagg gcgagaagat caagatgtgc gcggtcgagg cccaggaccg cgaattcggg
     4441 ctctacaaca agaaccccga cggcacgccc ctggcctgct cgaaggtgat ggcgaccggc
     4501 aattggtggt acctcgatcc gtcgtgcggc tgcggaccgg agggggcgta ttgccagccg
     4561 tcgcacatcg agtaccggac caacgccgag acgcgctccg agtaccggct gcgtcgcgcg
     4621 ctcgaggagg agccgctgca gatcatcggc tcggtggtcg cgcgcgacga ggattatctg
     4681 tcgatcctca cgacggcgcg cagcttcgtc aacggtccgc tggcgttcgc gttcctgcac
     4741 caggccgacg tgctgcgcgg cgagggcccg ctgcggatct ccccgcccgg gcccgaggac
     4801 ccggtgtgga gtcaggtgcc cttcgagagc gatgcatggg tcgagacgac gcgcccgtcg
     4861 caccactccg gcatcctgac gaccctcggc tacctgatcc ggttcccgac ctaccgcgcc
     4921 cgcatcgccc agtatcgccg caaattcctg tgcagcgcgg aattcgacta cgcgccccag
     4981 cccgaccccg cggaccacaa ccccgacatc gccgagcgca gcgggtgcag caactgccac
     5041 gcgcggctcg aattcgacgg catgtacttc ggccgctacc ccgaccgcac cggcctgtac
     5101 ctcgaccccg agaagttccc gatcgtcaac aagg
//
LOCUS       DASWNJ010000555         4214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_45212, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000555 DASWNJ010000000
VERSION     DASWNJ010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45212"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VGB85_09370"
     CDS             <1..822
                     /locus_tag="VGB85_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HEY0134279.1"
                     /translation="TTSAGAWAKDVVVPLEQPHNDIHLALGGFNAPNLDMGGFPDGSG
                     QVAGANGDMGENNTAALDPIFYFHHCNIDRLFWLWQKRNGHTDKLDVIKGYPGTNSSD
                     SQGPTPGVAPGTALDVDSPLSPFSNPKTNVPLTSNDVVNIETQLGYTYGPGSFDEPRA
                     RVAAAVKRGGSTKRLTVRGIDRALFQGSFVVSAFATIRENGASTTRFLGHHSVLSRWS
                     VIKCANCRTHLEVIAQFSLATLTDDEVKDATFSVKLQHRGVTPPRNLKIETSVGD"
     gene            981..2117
                     /locus_tag="VGB85_09375"
     CDS             981..2117
                     /locus_tag="VGB85_09375"
                     /inference="COORDINATES: protein motif:HMM:NF040195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134280.1"
                     /translation="MATPSPLFSQPSAQVSLDSPRIVSAQEHQRSGRRALPSFGLRFP
                     TRWRPAPLQNPHADAVERETLRWLAAHGIGVDADEREKLRQFNCAMYGGYSLPTSDYE
                     ETLLVTQFISLWLFWDDMQVEEHQDWDIDEVVRALDGEAGSSSRYVAAWADIGRRLQA
                     KRSRRWLRHLACTMRQWLENAKFETGLAKANQRGVCPDFQVLFECRTISIGMFPTFHL
                     IEHAEGFELPDAFHQHETTIAIKRLASRLVGLGNDLGGLAKDLENRWLNLVLALTDGS
                     GLPLAAAFQRVVDIHNADVAEMDRAAARLPSFGAAVDALAQRWVQAVRYNVHGFTLWE
                     ATAERYQARKVHIGDTALVAPVVYVVDPRRGEVSLVPYDRRQVA"
     gene            complement(2143..3543)
                     /locus_tag="VGB85_09380"
     CDS             complement(2143..3543)
                     /locus_tag="VGB85_09380"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY0134281.1"
                     /translation="MRHDETLDESKGARRRTAGPPLPGLVLIFSVDAPVISAIPLDTD
                     VLEIGRDTDALASLRDPLLSRRHARVERSDGRFVIHDVGSRNGTVVDGERLSGAATLV
                     PGQVIRTGGTIFLLCADITPYLRGVQVTADAVIGPLLRDTYDAIARAARHSRTLHVSG
                     ESGAGKEHAARVFHAAGSPAHGPFVAVNCAAIPEGVAERLLFGAKKGAYSGASADTDG
                     YVQTADGGTLFLDEVGDLQHEVQAKLLRVLETREVLALGAPRPHRVDVRFCTATHRDL
                     RLEVAAGRMREDFYFRIGRPEVAVPPLRARLEEIPWLLDRELRRVDPNLELSASLVET
                     CLLRPWPGNIRELLSEARAAALEALDGGSREVTSAHLSRLAGRALARAQPAATAPTEA
                     ASAPQAAPDRGVERPAPAVERRPRRHAPPTKAELSALMEEHGGVLQDVASALGCSRRQ
                     VGRWLDAHRIERGRAR"
     gene            complement(3616..>4214)
                     /locus_tag="VGB85_09385"
     CDS             complement(3616..>4214)
                     /locus_tag="VGB85_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134282.1"
                     /translation="EATLRASLALGERLGEVLTTTNSRLYLALLLSRDAGRTREAFGL
                     ATEVMGRNSLADGVALGIRAQVLLDEGDPCGAEALARRSCEMLAAMPTLRQHPLAVSL
                     RALVVAGRADDACRLADEALADIDAAPGGYNEVALRIAVAEARVAAGDIAGARAAAGE
                     ALRRVELRASKISDIATREAYLGRLADNVRARALATEG"
BASE COUNT          612 a         1487 c         1524 g          591 t
ORIGIN      
        1 acgacctcgg ccggtgcctg ggccaaggac gtcgtggtgc ccctggagca gcctcacaac
       61 gatatccacc tcgcgctcgg cgggttcaac gcgccgaacc tggacatggg cgggttcccc
      121 gacgggtcgg ggcaggtcgc cggcgcgaac ggcgacatgg gcgagaacaa cacggccgct
      181 ctcgacccga tcttctattt ccaccactgc aacatcgatc gcctgttctg gttgtggcaa
      241 aagcggaacg gccacaccga caagctcgac gtcatcaagg gctaccctgg cacgaactcg
      301 agcgacagcc agggtcccac ccctggcgtc gcgcccggca cagcgctgga tgtcgactcg
      361 cccctcagcc cgttctcgaa tccgaagacg aacgtgcccc tcacctcgaa cgacgtcgtc
      421 aatatcgaga cgcagctcgg ctacacgtac ggaccgggct cgtttgacga gccgcgcgct
      481 cgcgtggccg ccgcggtgaa gcggggtggc agcacgaaga ggctcacggt gaggggcatc
      541 gaccgcgcgc tgttccaggg gtccttcgtg gtgagcgcgt tcgctaccat ccgcgagaat
      601 ggcgcgtcca cgacgcgctt cctcggtcac cactccgtcc tcagccggtg gagcgtgatc
      661 aagtgcgcga actgccgcac gcacctcgag gtcatcgcgc agttctccct cgcgaccttg
      721 acggacgacg aggtcaagga cgcgacgttt agcgtgaagc tgcagcaccg cggggtgacg
      781 ccccccagga acttgaagat cgagacctcg gtcggcgact gacgacgacg cgaggtcctc
      841 cggtccgcga ctgctcggcg gcgtgggccc gcgagtcgtg tgggcgcccg cgggcgcccg
      901 cgggcgggcg cgggcgggcg aacgtaactc gctgaaaaca agagaagatt gtctggcacc
      961 gcccctgcac aagcggcgac atggcgaccc cgagcccact tttttctcaa ccatcggccc
     1021 aggtttccct cgacagtccg cggatcgtct ccgcgcagga gcaccagcgt tctggccggc
     1081 gggcgctgcc gtcttttggc ctgcgcttcc cgacgcggtg gcgtccggcg ccgctgcaga
     1141 atccgcacgc ggacgcggtc gagcgggaga cgctgcgatg gctcgccgcg cacggcatcg
     1201 gcgtcgacgc cgacgagcgg gagaagctgc gccagttcaa ttgtgcgatg tatggcggct
     1261 actcgctgcc gacctcggat tacgaggaga cgctgctcgt cacgcagttc atctcgctgt
     1321 ggctgttttg ggacgacatg caggtcgagg agcatcagga ctgggacatc gacgaggtcg
     1381 tgcgcgcgct cgacggggag gccgggtcgt cgtcgcggta tgtcgccgcg tgggccgaca
     1441 tcggccgccg actccaggcg aagcgcagtc ggcgatggct ccgccatctc gcgtgcacga
     1501 tgcggcagtg gctcgaaaac gccaagttcg agaccgggct cgccaaggcg aatcagcgcg
     1561 gcgtctgccc cgacttccag gtcctgttcg agtgcaggac gatcagcatc ggcatgtttc
     1621 cgaccttcca tctcatcgag catgccgagg gcttcgagct gccggacgcg tttcatcagc
     1681 acgagacgac gatcgccatc aagcgcctgg cgtcgcgcct cgtcgggctc ggcaacgacc
     1741 tcggcgggct ggccaaggac ctcgagaacc ggtggttgaa cctcgtgttg gcgctgacgg
     1801 acggctcggg gctcccgctg gcggccgcgt tccagcgcgt cgtcgacatc cataacgccg
     1861 acgtcgccga gatggaccgg gcggcggcgc ggctgccgag cttcggcgcc gcggtcgacg
     1921 cgctcgcgca gcgctgggtg caggcggtcc gctacaatgt gcacggcttc acgctgtggg
     1981 aggcgacggc ggagcgctat caggcccgca aggtgcacat cggcgacacc gcgctcgtcg
     2041 ctcccgtcgt ctacgtcgtc gacccgcggc gcggcgaggt gtcgctcgtc ccgtacgacc
     2101 ggcgacaggt cgcttgagag agcgagcccg gcgcctgtgg gctcaccgcg ctcgcccgcg
     2161 ctcgatccga tgcgcgtcga gccagcgacc gacctgccga cgcgagcagc cgagcgcgct
     2221 cgccacgtcc tgcaagacgc cgccgtgctc ctccatcagc gccgacagct ccgccttggt
     2281 gggcggtgcg tgacgcctgg ggcggcgctc gacggcgggg gccgggcgct cgacgccccg
     2341 gtcgggggcc gcctggggcg cgctcgcggc ctccgtcggc gcggtcgcgg cgggctgcgc
     2401 tcgcgcgagc gcgcggcctg cgagccggga gaggtgggcg ctcgtcacct cccggctccc
     2461 gccgtcgagc gcctcgaggg cggccgcacg cgcctcgctc aggagctcgc ggatgttgcc
     2521 gggccacggg cgcagcaggc acgtctcgac cagcgaggcg gacagctcga ggttcgggtc
     2581 gacgcgtcgg agctcgcggt cgagtaacca tgggatctcc tcgagtcgcg cgcgcaatgg
     2641 cgggacggcg acctcgggcc tgccgatgcg aaaatagaag tcctcgcgca tgcggccagc
     2701 ggcgacctcg aggcgcaggt cgcggtgcgt ggcggtgcag aagcgtacgt cgacgcgatg
     2761 cgggcgcggg gcgccgagcg cgagcacctc gcgcgtctcg agcacacgaa gcagcttggc
     2821 ctgcacctcg tgctggaggt cgccgacctc gtcgaggaag agcgtcccgc catcggcggt
     2881 ctggacatag ccatccgtgt ccgcggacgc gccggagtag gcgccctttt tggccccgaa
     2941 gagcagccgc tcggccacgc cctcggggat cgcggcgcag ttgaccgcga cgaacggccc
     3001 gtgtgccggc gatcccgcgg catggaacac gcgcgccgcg tgctccttgc cggcgccgct
     3061 ctcaccggag acatgaagcg tccggctgtg acgcgccgcg cgggcgatcg cgtcgtaggt
     3121 gtcgcgcagc agcgggccga tgacggcgtc cgcggtcacc tggacgccac gaagatacgg
     3181 cgtgatgtcg gcgcacagga ggaagatggt cccgccggtg cggatgacct gcccgggcac
     3241 cagggtggcg gcgcccgaga gccgctctcc gtcgaccacc gtcccgttgc ggctgccgac
     3301 gtcgtggatg acgaagcggc cgtccgagcg ctcgacgcgg gcgtgtcgac gcgagagcag
     3361 cgggtcgcgc agagacgcga gggcgtcggt gtcgcggccg atctccagca cgtcggtgtc
     3421 gagcgggatc gccgagatca ccggcgcatc gaccgagaag atcagcacga ggccgggcag
     3481 aggcgggccc gcggttcgac gcctggcgcc cttcgactcg tcgagggtct cgtcgtgcct
     3541 catgcgctca cccggccgga agcgccgcgg cgtccggcgc acttccgcgg agttgcgtgc
     3601 gctcgcgggg tcacgtcagc cctccgtcgc cagcgcgcgc gcgcggacgt tgtcggcgag
     3661 acggccgagg tacgcctcgc gcgtggcgat gtccgagatc ttgctcgccc gcagctcgac
     3721 gcggcgcagg gcctcgcccg cggccgcgcg cgcgcccgcg atgtcaccgg cggcgacgcg
     3781 ggcctcggcg acggcgatcc gcagcgccac ctcgttgtag ccgccggggg cggcgtcgat
     3841 gtccgccagc gcctcgtccg cgagccggca cgcatcgtcc gcgcgcccgg caacgacgag
     3901 cgcccgcagc gagaccgcga gcgggtgctg gcgcagcgtc ggcatcgcgg ccagcatctc
     3961 gcaggaccgg cgcgcgaggg cctcggcgcc gcacgggtca ccctcatcga ggaggacctg
     4021 cgcgcgaatg ccgagcgcga cgccgtcggc gagcgagttc ctgcccatga cctcggtggc
     4081 gagcccgaaa gcctcgcgcg tccggcccgc gtcgcgcgac agcagcagcg ccaggtagag
     4141 cctggagttc gtggtcgtca gcacctcgcc caggcgctcc ccgagcgcga ggctcgcgcg
     4201 aagcgtcgcc tcgc
//
LOCUS       DASWNJ010000556         1918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_396506, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000556 DASWNJ010000000
VERSION     DASWNJ010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396506"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="VGB85_09390"
     CDS             <1..873
                     /locus_tag="VGB85_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134283.1"
                     /translation="NGQEVRGQCGELIINDPISVDTDALLKVYPGCVPLDVTLAQLQS
                     DGIIYGGDGTPITCELKPGRGNGSDNYDNDPETPSTHPPGLTLTDACAVTGTISPSLP
                     YGVYAFITTFTQSGQFAYVPYCAAQMVQPGSAYDIIREDTGTDATFKPGVVVLGEGEM
                     LSYGTNVPDPKVTVTDDVGACANNTCFYSFVFGYNTLSSMFSSVSASPNSKFPAQGFD
                     GFTHALIVKDNALDIFRGRAYVTNIRFDYCIADNADDCGNDPKYTAAQRGELVRQNGG
                     GSNYYFSLVALPAN"
     gene            955..>1918
                     /locus_tag="VGB85_09395"
     CDS             955..>1918
                     /locus_tag="VGB85_09395"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0134284.1"
                     /translation="MRDRRVARVIDREIAPIWHDRFARLIIRNLPGASDVIALDIHCG
                     VGRTTAELLGRLGSAARVLAIEPDDTLIEFAKTRMRPEWKNRVYLKPGNFDDVTAMAD
                     DSYNLVVANLVLGDAGDIGSAIAEMIRVNRIGGRILATLPMAGSWHEVEDIFAEVLRD
                     AGLTAAVRRLERLRELRPTGAQLANIVRGASVAEDDFVIEQERFQLLFPSGREFLFAP
                     VVEHGPLRLWKAVIGKDGSPQELFWRLKEAIDTYYTGHVLAVNVMAGLMNIHVAKGTE
                     SPGPRLAARHWRHYPTLDALWGGLAAGYVRVSEPNLPSFSDGDFD"
BASE COUNT          326 a          724 c          591 g          277 t
ORIGIN      
        1 aacggccagg aagtccgcgg gcagtgcggt gagctgatca tcaacgaccc gatctctgtc
       61 gacaccgacg cgctgctcaa ggtctacccg ggctgcgtcc cgctcgacgt caccctcgcc
      121 cagctccagt ccgacggcat catctacggc ggcgacggca ccccgatcac ctgcgagctc
      181 aagcccggtc gcggcaacgg cagcgacaac tacgacaacg acccggagac cccgagcacg
      241 cacccgccgg gcctgacgct gaccgacgcc tgcgccgtca ccggcacgat cagcccgtcg
      301 ctgccctacg gcgtctacgc gttcatcacc accttcacgc agagcggcca gttcgcgtac
      361 gtcccctact gcgccgcgca gatggtccag cccggcagcg cctacgacat catccgcgag
      421 gacaccggca cggacgccac cttcaagccc ggcgtcgtcg tgctcggcga aggcgagatg
      481 ctgtcctacg gcaccaacgt gcccgacccc aaggtcaccg tcaccgatga cgtcggcgcc
      541 tgcgccaaca acacctgctt ctacagcttc gtgttcggct acaacacgct gtcctcgatg
      601 ttctcgagcg tgtccgccag cccgaactcg aagttcccgg cccagggctt cgacggcttc
      661 acccacgccc tgatcgtcaa ggacaacgcg ctcgacatct tccgcggtcg cgcctacgtc
      721 accaacatcc gcttcgacta ctgcatcgcc gacaacgcgg acgactgcgg caacgacccg
      781 aagtacaccg cggcccagcg cggcgagctg gtccgccaga acggcggcgg cagcaactac
      841 tacttcagcc tggtcgcgct gcccgccaac tgaggcgcgg cgagcaccac aggcccatcc
      901 ccggcgaaat atcgtttgag cagtcaggac agtcgcggag gcggggccgc cagcttgcga
      961 gatcgccggg tcgcccgggt catcgatcgc gagatcgcac cgatctggca cgaccgcttc
     1021 gcccggctga tcatccgtaa cttgccgggc gcgagcgatg tcatcgcgct cgacattcac
     1081 tgcggggtcg ggcgcaccac cgccgagctg ctcggccggc tcggcagcgc cgcgcgcgtg
     1141 ctcgcgatcg agcccgacga caccctgatc gagttcgcca agacgcgcat gcgtccggag
     1201 tggaagaacc gggtctacct caagcccggc aacttcgacg acgtcacggc gatggccgac
     1261 gacagctaca acctcgtcgt cgccaacctc gtgctcggcg acgccggtga catcggctcg
     1321 gcgatcgccg agatgatccg cgtcaatcgc atcggcgggc gcatcctcgc taccctgccg
     1381 atggccggct cgtggcacga ggtcgaggac atcttcgccg aggtcctgcg cgacgccggc
     1441 ctgaccgccg cggtccgccg cctcgagcgc ctgcgcgagc tgcgacccac cggcgcccag
     1501 ctggccaaca tcgtgcgcgg cgccagcgtc gccgaggacg acttcgtcat cgagcaggag
     1561 cgcttccagc tgctgttccc gagcggccgc gagttcctgt tcgcgccggt ggtcgagcat
     1621 ggaccactac gcctgtggaa ggccgtcatc ggcaaggacg gcagcccgca ggagctgttc
     1681 tggcggctca aggaggcgat cgacacgtac tacaccggcc acgtgctcgc ggtgaacgtg
     1741 atggccggcc tgatgaacat ccacgtcgcc aagggcaccg agagcccggg cccgcggctc
     1801 gccgcgcggc actggcggca ctacccgacc ctcgacgcgc tatggggcgg gctcgcggcc
     1861 ggctacgtgc gggtctccga gccgaacctg ccgtcctttt ccgacggcga cttcgact
//
LOCUS       DASWNJ010000557         5292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_115933, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000557 DASWNJ010000000
VERSION     DASWNJ010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115933"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VGB85_09400"
     CDS             complement(<1..704)
                     /locus_tag="VGB85_09400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0134285.1"
                     /translation="MQSRLQDRVCIITGATGGIGAAMARRLAAEGARLMLVDIQGTPL
                     AALAAEFAADWAHADVADDAAVKAYTEQTLARYGRIDAVLANAGTEGRVAPIVDSRPE
                     DVARLFAVNVFGPFSAIRHAAPHMQRGGTIIVTASVASFVGSPGLAPYCASKHALLGL
                     VRSAAQELGPRGIRVNAIHPGPVDNRMMRSIEEQAAPGHAETVKDDFAARVPLGRYAS
                     NEEIAAMASFLVSDDSA"
     gene            993..1412
                     /locus_tag="VGB85_09405"
     CDS             993..1412
                     /locus_tag="VGB85_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0134286.1"
                     /translation="MTAVESPRAPVVVVDDEPRTAQVLAELLAMDGLDVEVCAAARLS
                     DRLAGGPTPGGLVMNVSMARADDVRVVRQVRAEHPALPVFIVTEYPHLATRLGPQLAA
                     QAAVFTKPVDYPALLGVLRMATAPLHLQMAQAQESPW"
     gene            1429..3039
                     /locus_tag="VGB85_09410"
     CDS             1429..3039
                     /locus_tag="VGB85_09410"
                     /inference="COORDINATES: protein motif:HMM:NF022995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HEY0134287.1"
                     /translation="MKHLPPLETQAEELKRYVDFSAEDAALLRSFHPVAAPHFERIAQ
                     EFYDRIRRHEEAHAVFTGEAQIARLQRSLVAWMHRLFGGQYDEQYFADIAKIGRIHVK
                     VGLPQRYMFTAMALIRTSLTQIAERDRPDGAAVSATLSRLLDVELAVMLESYRDDFVA
                     RLQHIERLERDALGASLARTEHRYVNAVELAQVLIVGLDGAGTIRLFNREAERVTGFA
                     RDEVLGTRIVEQLGIDMFVPDAWTADPHGEPKFAAPLETAVRTRSGNTRIVLWQLSHA
                     PSQGDEVCMFALGRDITDEKALHARTQQQEKLAAIGTLAAGLAHEIRNPLNGAQLHVT
                     YLSRSLKKANANPELVDTVAVVADEIARLGTLVTEFLAFARPRPLQIRPVTVQALLGR
                     VSQLAPAGEHATAIVLDVPGAAIELQADAAKLEQVLLNLTQNAVDAIAGAAGGQVVLR
                     ARRQPRTVTIEVEDNGPGIAAGTPVFDAFFSTKPQGTGLGLSISHRIVTDHGGTLDVD
                     SRGGRTIFRVVLPIDGPPAMDRMPSSGA"
     gene            3049..4407
                     /locus_tag="VGB85_09415"
     CDS             3049..4407
                     /locus_tag="VGB85_09415"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0134288.1"
                     /translation="MTQHARGRILVVDDEASARNGLEKLLRQEGFDVRAEADGADGLR
                     AAEEFAPDVVVTDLRMPKMDGMQLLARLREQDSGMPVIVATAFGEVASAVAAMRAGAD
                     DYLMKPIDIDGLVLAIERAIERRSLRVEAENLRRQLRERDGSGLQGLLGTSPAMQKVY
                     RVARQVAGSRATVLMTGESGTGKGELARAIHALSPRAKEPFITLHCAALAESLLESEL
                     FGHEKGSFTGADRKRLGRFEQADRGTLFLDEVGEIPPAMQVKLLRVLQERTFERVGGG
                     ESIKVDVRLVAATNRDLAKEVREGRFREDLYYRLNVVHVELPPLRLRGRDSLLLAEHF
                     LQRFTEENHASIKGFTEAARAKLTRHTWPGNVRELENAVERAVVLCDGDEIDAEHLPF
                     EGAPDVQGGVRIPGSTMAEIERHAILTTLDAVDGSTARAAEVLDISVRTIQYRLHAYG
                     RR"
     gene            4531..5283
                     /locus_tag="VGB85_09420"
     CDS             4531..5283
                     /locus_tag="VGB85_09420"
                     /inference="COORDINATES: protein motif:HMM:NF042511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6544 family protein"
                     /protein_id="HEY0134289.1"
                     /translation="MTHFARDRVFDELWAQDGAGATFDPGSVAGLPDAAQRYLTHAIA
                     PGTPRASAVRLQMHGEIKLGKWRPFVAEEVLRWDRGFVWRARTRIAGLPVGGSDRWVD
                     GRGAMQWKMLGLIPVMVGAGPDISRSALGRAQLEVVWLPSALLAPEVAWQPGDDGRLD
                     ATRTFHGEPGRVSLTTDAQGRLRTVAGLRWGDPDRVGHRLVPFGGHVEEERSFDGYTI
                     PSRLRLGWFFGTDRFEPEGEFFRATISRAEFR"
BASE COUNT          765 a         1868 c         1900 g          759 t
ORIGIN      
        1 gcgctgtcgt cgctgacgag gaagctcgcc atcgccgcga tctcctcgtt gctcgcgtag
       61 cgaccgagcg ggacgcgggc ggcgaagtca tccttgaccg tctcggcgtg gccgggcgcg
      121 gcctgctcct cgatcgaccg catcatgcga ttatcgaccg ggccagggtg gatcgcgttg
      181 acgcggatcc cccgcgggcc gagctcctgc gcggcgctgc gcaccagccc gagcagcgcg
      241 tgcttgctgg cgcagtacgg cgcgagcccc ggagacccga cgaacgaggc gaccgacgcg
      301 gtgacgatga tcgtgccgcc gcgctgcata tgcggcgcgg cgtggcggat cgccgagaag
      361 gggccgaaca cgttgaccgc gaacagccgc gcgacgtcct cgggccggga atcgacgatc
      421 ggcgcgacgc gaccctcggt cccggcgttc gccagcacgg cgtcgatccg cccgtaacgt
      481 gcgagcgtct gctcggtgta ggccttgacc gcggcgtcgt cggcgacgtc cgcgtgcgcc
      541 cagtcggccg cgaattcggc cgccagcgcc gcgagcggcg tgccctggat gtcgacgagc
      601 atcaggcgag cgccctccgc cgcgagccga cgcgccatgg cggcgccgat gccaccggtg
      661 gcgcccgtga tgatgcagac cctgtcctgt agtctcgatt gcataaagtc ctggacccgt
      721 ctgagcaagc ggcgtgccag cgctgccccg gtgttcaaca gccaccggcc ggcactccgc
      781 gcggctcgtc gaggcaaggc ttgcacctcg acgcaagaac tgcatctcgc gccgatcggg
      841 cccatccagg gcctgcgtgg cgtcgctgcg accctcgcgg ccacgcaacg cgcatgatga
      901 aagaactatg cgcattgaaa tcacaccgtt tctccgccgg cacgccggat gctcgtcgca
      961 gcgtcgtcgc catgaccagc ccgctcccag tcgtgaccgc cgtcgagtcg ccgcgtgcgc
     1021 cggtcgtcgt cgtcgatgac gagccgagga ccgcccaggt gctcgcggag ctgctggcga
     1081 tggacggcct cgacgtcgag gtgtgcgcgg ccgcgcggct gagcgaccgg ctcgcgggcg
     1141 gcccgacccc gggtgggctg gtcatgaacg tctcgatggc gcgcgcggac gacgtgcggg
     1201 tggtgcgtca ggtccgcgcc gaacaccccg cgctgccggt cttcatcgtc accgaatacc
     1261 cgcacctcgc tacgcgcctc ggaccgcagc ttgccgcgca ggcggcggtg ttcaccaaac
     1321 ccgtggatta cccggcgctg ctcggcgtgc tgcgcatggc cactgcaccc cttcatttgc
     1381 agatggcgca ggctcaggaa tcaccatggt agcgcagcgg cgggcccgat gaagcacctg
     1441 ccgccactcg agacgcaggc cgaggagctc aagcgttacg tcgacttctc ggccgaggac
     1501 gccgcgctgc tgcgttcgtt tcatcccgtc gcggcgccgc acttcgagcg catcgcccag
     1561 gaattttatg atcggatccg ccggcacgag gaggcccacg cggtgttcac cggcgaggcc
     1621 cagatcgcca ggttgcagcg ctcgctggtc gcgtggatgc accggctgtt cggcgggcag
     1681 tacgacgagc agtacttcgc ggacatcgcc aagatcggcc ggatccacgt caaggtcggc
     1741 ctgccgcagc gctacatgtt caccgcgatg gccctgatcc gcacgtcgct gacgcagatc
     1801 gccgagcgcg accggcccga cggcgcggcc gtgtcggcga cgctctcgcg gctgctggac
     1861 gtcgagctcg cggtcatgct cgagagctac cgcgacgact tcgtcgcccg cctgcagcac
     1921 atcgagcgcc tcgagcgtga cgccctcggc gcctcgctcg cgcgcaccga gcatcgctac
     1981 gtcaacgccg tcgagctcgc gcaggtgctg atcgtcgggc tcgacggcgc cggcacgatc
     2041 cgcctgttca accgcgaggc cgagcgcgtc accggcttcg cgcgcgacga ggtgctcggc
     2101 acccgcatcg tcgagcagct ggggatcgac atgttcgtcc ccgacgcctg gaccgccgac
     2161 ccgcacggcg agccgaagtt cgcggccccg ctcgagacgg ccgtgcgcac gcgatccggc
     2221 aacacgcgga tcgtgctgtg gcagctctcg catgcgccgt cgcagggcga cgaggtgtgc
     2281 atgttcgcgc tcggccgcga catcaccgac gagaaggcgc tgcacgcgag gacccagcag
     2341 caggagaagc tggcggcgat cggcacgctc gcggccggcc tcgcccacga gatccgtaac
     2401 ccgctcaacg gggcccagct gcacgtcacc tacctgtcgc gctcgctcaa gaaggccaac
     2461 gccaaccccg agctcgtcga cacggtcgcg gtcgtcgccg acgagatcgc gcggctcggc
     2521 acgctggtga ccgagttcct cgcgttcgcg cggccgaggc cgctgcagat ccgtccggtg
     2581 acggtgcagg cgctgctcgg gcgggtctcg cagctcgcgc ccgcgggtga gcacgcgacg
     2641 gcgatcgtgc tcgatgttcc aggcgccgcg atcgagctcc aggccgacgc cgccaagctc
     2701 gagcaggtcc tgctcaatct tacacagaac gcggtcgacg cgatcgccgg cgcggccggt
     2761 ggacaggtcg tgctgcgcgc gcgccgacag ccgcgcaccg tcacgatcga ggtcgaggac
     2821 aatggccccg ggatcgcggc cgggaccccg gtgttcgacg cgttcttctc gaccaaacca
     2881 cagggcaccg gcctcggcct gtcgatctcg catcgcatcg tcaccgatca cggcggcacc
     2941 ctcgacgtcg acagccgcgg cggccgcacg atctttcgcg tcgtgctgcc gatcgacggc
     3001 ccgccggcga tggaccgcat gccctcgtcg ggcgcataaa gggagcccat gacccaacac
     3061 gcgagaggcc ggatcctcgt ggtcgacgac gaagccagcg cccgcaacgg cctggagaag
     3121 ctgctgcgcc aggagggctt tgatgtccgc gccgaggccg acggcgccga cggcctgcgc
     3181 gccgcggagg agttcgcgcc ggacgtggtg gtcaccgacc tgcgcatgcc gaagatggac
     3241 ggcatgcaat tgctggcgcg cctgcgcgag caggacagcg gtatgccggt gatcgtggcg
     3301 accgcgttcg gcgaggtcgc ctcggcggtc gcggcgatgc gcgcgggcgc cgacgactac
     3361 ctgatgaagc cgatcgacat cgacggcctg gtgctggcga tcgagcgggc gatcgagcgt
     3421 cgctcgctgc gcgtcgaggc cgagaacctg cgccggcaat tgcgtgagcg cgacggcagc
     3481 ggcctgcaag ggctgctcgg gaccagcccg gcgatgcaaa aggtgtatcg cgtggcgcga
     3541 caggtcgcag gttcgcgggc gaccgtgctg atgaccggcg agagcggcac cggcaagggc
     3601 gagctggcgc gcgcgatcca tgcattgagc ccgcgggcaa aggagccgtt catcaccctg
     3661 cactgcgccg cgctcgcgga gtcgctgctc gagagcgagc tgttcggcca cgagaagggg
     3721 tcgttcaccg gcgccgaccg caagcggctc gggcgcttcg agcaggccga ccgcggcacc
     3781 ctgttcctcg acgaggtcgg cgagatccca ccggcgatgc aggtcaagct gctgcgcgtg
     3841 ctgcaggagc ggaccttcga gcgcgtcggc ggcggcgagt cgatcaaggt cgatgtccgc
     3901 ctcgtcgccg cgaccaaccg cgatctcgcg aaggaggtcc gcgagggccg gttccgcgag
     3961 gacctgtatt atcgcctcaa cgtcgtgcac gtcgagctgc cgccgctgcg cctgcgcggg
     4021 cgcgactcgc tgctgctcgc cgagcatttt ttgcagcgct tcaccgagga gaaccacgcg
     4081 agcatcaagg gcttcacgga ggcggcgcgg gccaagctga cgcgccacac ctggccgggc
     4141 aacgtgcgcg agctcgagaa cgcggtcgag cgcgcggtgg tgctgtgcga cggcgacgag
     4201 atcgacgcgg agcacctgcc cttcgagggc gcgccggacg tgcagggcgg ggtgcggatc
     4261 cccggctcga cgatggcgga gatcgagcgc cacgcgatcc tgacgacgct cgacgcggtc
     4321 gacggctcga ccgcgcgggc cgccgaggtc ctcgacatca gcgtgcgcac gatccagtac
     4381 cgcctgcacg cctacgggcg gcggtgaacg ccgcgggcgg ccctgacgca ggttctgcac
     4441 cacgatgcat gcgctgcgcc gtgtgagcgc cgcgtgggcc gtgtgggccg cgtcgtcacg
     4501 cctggtccga tgtttgctac cacgcccgcg atgacgcact ttgcgaggga tcgagtgttc
     4561 gacgagctat gggcgcagga cggtgcgggc gcgacgttcg accccgggag cgtcgcgggt
     4621 ctgcccgacg cggcgcagcg ctatctgacc cacgcgatcg ccccgggcac gccgcgcgcc
     4681 tcggccgtgc gcctgcagat gcacggagag atcaagctcg gcaagtggcg cccgttcgtc
     4741 gccgaggagg tgctgcgctg ggatcgcggc tttgtctggc gcgcgcgcac acgaatcgcc
     4801 ggtctgccgg tcggtggctc ggatcgctgg gtcgacggcc gcggcgcgat gcagtggaag
     4861 atgctcggcc tgatcccggt gatggtgggg gcgggcccgg acatctcgcg ctccgcgctc
     4921 gggcgggccc agctggaggt cgtgtggcta ccctcggccc tgctcgcgcc ggaggtggcc
     4981 tggcagcccg gggacgacgg ccggctcgac gcgacgcgca cgttccacgg cgagcccggg
     5041 cgcgtctcgc tgacgaccga cgcgcagggc cgcctgcgca cggtcgccgg gctgcgctgg
     5101 ggtgaccccg accgcgtggg tcatcgcctc gtgccgttcg gcgggcacgt cgaggaggag
     5161 cgcagcttcg atggttacac gatcccctcg cgcctgcggc tcgggtggtt cttcggcacc
     5221 gaccgcttcg agcccgaggg cgagttcttc cgcgcgacga tctcccgggc cgagtttcgc
     5281 tgagcgatca gg
//
LOCUS       DASWNJ010000558         2205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_115952, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000558 DASWNJ010000000
VERSION     DASWNJ010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115952"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VGB85_09425"
     CDS             <1..314
                     /locus_tag="VGB85_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134290.1"
                     /translation="HPARIQRNMQAEGAYVSLYWYGSPVSRSKLRAARRIVAVDGKPT
                     PDLDAFLAAVADKPNHASVRLKTVDLDDKIELITLRLDLDYFPTYELRHTPAGWIRRE
                     L"
     gene            370..858
                     /locus_tag="VGB85_09430"
     CDS             370..858
                     /locus_tag="VGB85_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134291.1"
                     /translation="MGITRICPIARILAACGVMACGAAPLGNDGAQGGGSKGGDFSDP
                     AIEDRCHESAELSVFAAERHCECLVAAGEHPDQATCVAERSPAATDVDCVCSTYASYP
                     ESSAFIDCFIPIQQAAVDCAAEALCNTAKLDACNAKFADYTQCPLPTEAADEVSEQCE
                     LP"
     gene            964..>2205
                     /locus_tag="VGB85_09435"
     CDS             964..>2205
                     /locus_tag="VGB85_09435"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0134292.1"
                     /translation="MQNGRDMFGAGPQGLDRTLAGGPATSRVAPSPTTLPGDLGGTLS
                     EAGPEPVDDDDIHIGGLQRGAAIGRFVMLELLGSGGMGEVFLCYDPELDRRVAVKLLR
                     PIRGGRAGEAPARLLREARAIARLSHPNVVTVHDVALWEGRVFLAMEYVAGPTLTAWA
                     RDHARDPAAIVAVYRQAGEGLAAAHRAGLVHRDFKPDNVLVAEGCRPRVLDFGIARTA
                     ESDDDAPALNSASTLAVIDPSIDLTTTGMLLGTPAYMAPEQLSGLPVDARTDQFSFCV
                     SLWEALHGQRPFAGDDLATLTSNVLQGRIRPPPREARVPERIEKALRRGLSLDAAARF
                     PDMPALLAALAPPSAGSGRWFVALAVIGLAAGATAWSIGGDDPAAACTGDAGLLASAW
                     DPPLRDAGAAAFASSGAPRADE"
BASE COUNT          288 a          870 c          744 g          303 t
ORIGIN      
        1 accacccggc gaggatccag cgcaacatgc aggcggaggg cgcctacgtc tcgctgtact
       61 ggtacggctc gccggtcagc cgctcgaagc tgcgggccgc ccggcggatc gtcgccgtcg
      121 acggcaagcc gacccccgac ctcgacgcct tcctcgccgc cgtcgccgac aagcccaacc
      181 atgcctcggt gcgcctcaag accgtcgacc tcgacgacaa gatcgagctg atcacgctcc
      241 gcctcgacct cgactacttc ccgacctacg agctgcgcca cacccccgcc gggtggatcc
      301 gccgcgagct gtgaccgctt ggcacgccgg ccgcggcgtg cgaccctcgc ggggcacggg
      361 gagtggagca tggggatcac gagaatttgc ccgatcgcgc gcatcctggc cgcctgcggt
      421 gtgatggcct gcggcgccgc tccgctcggc aacgacgggg cccagggcgg cgggtcgaag
      481 ggcggcgact tcagcgaccc ggcgatcgaa gatcgctgcc acgagtccgc cgagctgtcg
      541 gtgttcgcgg ccgagcgcca ctgcgagtgc ctcgtcgccg ccggtgagca ccccgaccag
      601 gcgacctgcg tcgccgagcg ctcgcccgcg gcgaccgacg tcgactgcgt gtgctcgacc
      661 tacgcgagct accccgagtc gagcgcgttc atcgactgct tcatcccgat ccagcaggcc
      721 gccgtcgact gcgcggcaga ggccctgtgc aacacggcca agctggacgc gtgcaacgcg
      781 aagttcgccg actacacgca atgcccgctg cccaccgagg cagcggacga ggtgtcggag
      841 cagtgcgagc tgccctgatg tagtggtcgt gcgcccccgg tccgggcctg ctcaccattc
      901 gtgctgatgt gcgcatgtgc gcaccttact tttattcgtt tcggtatcat cgccgtcgcg
      961 cgtatgcaga acggtcgaga catgttcggc gccggaccgc agggcctcga tcgcaccctc
     1021 gccggcggcc ccgcgacatc acgggtggcg ccgagcccga ccacgctgcc cggcgacctc
     1081 ggcggcacgc tcagcgaggc cgggcccgag cccgtcgacg acgacgacat acacatcggc
     1141 gggctgcaac gcggcgccgc gatcggccgc ttcgtcatgc tcgagctgct cggcagcggc
     1201 ggcatgggcg aggtcttcct ctgctacgac cccgagctcg accgccgcgt cgccgtgaag
     1261 ctgctgcggc cgatccgggg cggtcgcgcc ggcgaggcgc ccgcgcggct gttgcgtgag
     1321 gcgcgggcga tcgcccggct ctcgcacccc aacgtcgtca ccgtgcacga cgtcgccctg
     1381 tgggagggcc gcgtattcct ggcgatggag tacgtcgccg gcccgacgct caccgcctgg
     1441 gcccgcgatc acgcccgcga tcccgcggcg atcgtcgcgg tgtatcgcca ggccggcgag
     1501 ggcctcgccg cggcccaccg cgccggcctc gtccatcgcg acttcaagcc cgacaacgtg
     1561 ctcgtcgccg agggttgccg gccgcgcgtg ctcgacttcg ggatcgcacg cacggccgag
     1621 tccgacgacg acgcgccggc cctgaactcg gcctcgaccc tcgccgtcat cgacccctcg
     1681 atcgacctga ccaccaccgg catgctgctc ggcaccccgg cctacatggc cccggagcag
     1741 ctgagcggct tgcccgtcga cgcgcgcacc gatcagttca gcttctgcgt ctcgctgtgg
     1801 gaggccctgc acggccagcg acccttcgcc ggcgacgacc tcgcgacgct gaccagcaac
     1861 gtcctgcagg gccgcattcg gccgccgccc cgcgaggccc gcgtgcccga gcgcatcgag
     1921 aaggcgctgc gccgcggcct ctcgctcgac gccgccgccc gcttccccga catgcccgcg
     1981 ctgctcgccg ccctcgcgcc gccgagcgcc ggctcgggcc gctggttcgt cgccctcgcg
     2041 gtgatcggcc tcgccgccgg cgcgaccgcg tggtcgatcg gtggtgatga cccggccgca
     2101 gcgtgcaccg gcgacgccgg cctgctcgcc agcgcgtggg accctcccct gcgcgacgcc
     2161 ggcgcggccg ccttcgccag cagcggcgcg ccgcgggccg acgag
//
LOCUS       DASWNJ010000559         1822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_540720, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000559 DASWNJ010000000
VERSION     DASWNJ010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540720"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1246)
                     /locus_tag="VGB85_09440"
     CDS             complement(128..1246)
                     /locus_tag="VGB85_09440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0134293.1"
                     /translation="MQLAGDQAPIHRQFRAIYRDEFAFVWAVARRFGVPVAALDDAVQ
                     DVFLTAFRRIDRLGYQVSSRAWLYAVTRKVASHHRRSAMRLDRRIEALRAVAPELPAA
                     PQERHHAADLLERLLAPLPRGLREVWEMTELLGMTGPEIAAELEVPLNTVYSRLRLAR
                     AQLSAGAKTIAACVAEATRHTAPPPEAAQRNWVVILPMLRPGSFTGIAAAWTTTRAAL
                     ATTLIASAAVVTVVASPGPRPAPRPVIAEPAPSIVAPAPAIVIPQRLAAAPAALPTPR
                     RPAPSPTDRLAAEVALLDAARDHLAAHAPAAALALLTEHARQFPDGALYDAREAARVE
                     AACQLGDAAAAEVTARALVARHTDSLVARKYQHYVCAP"
     gene            complement(1285..>1822)
                     /locus_tag="VGB85_09445"
     CDS             complement(1285..>1822)
                     /locus_tag="VGB85_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134294.1"
                     /translation="APPQRRSPGAMLRVHGGPEYGAVPGVTGAIGLATGLLWPRARLE
                     VHGLYLAPRTTTAGANALRLSLFSGAVLGCGRLGRGAFELPLCGGLELGGVRSTATGP
                     GARSGFDIWVAGALTAGIAWHVARRLSLTAALHGLVHLYGPRYELGDPGEGEALFRAA
                     PVSGRLLFGLELRLGDRW"
BASE COUNT          257 a          630 c          727 g          208 t
ORIGIN      
        1 cgcgagcgac agggcgaata atgaatgctg caacgtggtc gagatggcca agagatgtct
       61 gcctccatgg gtgtcatcgc cggctcgccg cgatcccgtc acgacgggcg aacaattatc
      121 gtcgcgatca cggggcgcac acgtaatgtt gatactttcg cgcgacgagt gaatcggtgt
      181 gtcgggcgac cagcgcgcgg gcggtcactt cggccgctgc cgcgtcgccg agctggcacg
      241 ccgcctcgac gcgcgcggcc tcgcgggcgt cgtacaaagc gccgtccggg aactgtcggg
      301 catgctcggt cagcagggcg agcgcggcgg cgggcgcgtg tgcggcgagg tgatcgcggg
      361 ccgcgtcgag taacgcgacc tcggcggcga ggcggtcggt cggcgacggg gcggggcgac
      421 gcggcgtcgg gagtgcagcc ggcgccgcgg caagccgctg cgggatgacg atcgcagggg
      481 ccggcgcgac gatcgagggc gcgggctcgg cgatgaccgg ccgcggcgcg ggcctggggc
      541 cgggtgaggc gacgaccgtg acgacggccg cgctggcgat gagggtcgtg gcgagcgcgg
      601 cgcgggtggt cgtccaggcg gcggcgatgc cggtgaagct gcccggtcgc agcatcggca
      661 ggatcacgac ccagttgcgc tgcgcggcct cggggggcgg ggcggtgtgc cgggtcgcct
      721 cggcgacgca ggcggcgatc gtcttggcgc cggcgctcag ctgggcccgc gcgaggcgca
      781 gccgcgagta gacggtgttg agcgggacct cgagctcggc ggcgatctcc ggcccggtca
      841 tgccgagcag ctcggtcatc tcccacacct cgcgtaggcc gcgaggcagc ggcgcgagca
      901 ggcgctcgag cagatcggcg gcgtggtgac gctcctgcgg ggccgcgggg agctcgggcg
      961 cgacggcccg gagcgcctcg atccgccggt cgaggcgcat ggcgctacgg cgatggtgcg
     1021 aggcgacctt gcgggtgacc gcgtagagcc acgcacgcga cgagacctga taaccgaggc
     1081 gatcgatccg gcgaaacgcg gtcaggaaga cgtcctggac cgcgtcgtcg agcgcggcga
     1141 ccggcacccc gaagcggcgc gcgacggccc acacgaacgc gaactcgtcg cggtagatcg
     1201 cgcgaaattg gcggtggatc ggcgcctggt ccccggcgag ttgcatggcc tcccggaccg
     1261 tatgcccggc tcgccccggt tccgtcacca tcggtccccg agccgtaact cgagcccgaa
     1321 cagcagcctc ccggacaccg gggccgcgcg aaacagcgcc tcgccctcgc ccgggtcgcc
     1381 cagctcgtag cgcgggccgt agagatgaac gaggccgtgc agcgcggccg tcaggctcag
     1441 cctgcgggcg acgtgccagg cgatgcccgc agtcagcgcg ccggccaccc agatgtcgaa
     1501 gcccgagcgc gcgcccggac ccgtcgcggt cgaccgcacg ccgccgagct cgaggccgcc
     1561 gcacagcggg agctcgaagg cgccgcggcc gagtcgcccg cagccgagca cggcgccgga
     1621 gaacagcgac aggcgcagcg cgttggcgcc cgccgtcgtg gtccgcggcg cgaggtagag
     1681 gccgtgtacc tcgaggcgcg cgcgcggcca cagcagcccg gtggcgagcc cgatcgctcc
     1741 ggtgacgccc ggcacggcgc catactcggg cccgccgtgg acccgcagca tcgcgcccgg
     1801 agagcgtcgc tgtggcggcg ca
//
LOCUS       DASWNJ010000560         3074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_293198, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000560 DASWNJ010000000
VERSION     DASWNJ010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293198"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..1188
                     /locus_tag="VGB85_09450"
     CDS             325..1188
                     /locus_tag="VGB85_09450"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HEY0134295.1"
                     /translation="MVGDACDTCPLEAGDACTMLDADDIDDDGVPNGSDNCPRVANAN
                     QADAESDGHGDECDSCAEYNPGPGVCPTTIPAIRNPMNPGHPAPGASVSITGAYVTAL
                     RPDAGGTRGFYVQNDTSEPWNGIFVFTASKPAPVKLGNRVALTGIYEEFNGLAEITNP
                     TVTIEDPGLVLPFDPILLDPATLSTGKANAEPYESMLVHIGPVAITDDNADDPMDFDE
                     FVVTGALRVDDQISDNLKDMVLNNKCAIGTKFDDIIGVLGYSFANSKLQPRFKADIVL
                     GAMNMCDPFAP"
     gene            complement(1345..>3074)
                     /locus_tag="VGB85_09455"
     CDS             complement(1345..>3074)
                     /locus_tag="VGB85_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134296.1"
                     /translation="DSSSGGATPTTGSPEPTGTGGVTVDPTSDSGGGGQLVIDPAKAT
                     ITLEDGVAPPLQLTAKLDGTPVTAVWTSEASFLGGVDDSGLVTVSGNYGGQIQIEASH
                     QGQQAEAEIDVLFKAVVAPPEMTEADKLQLDGAINPDAEAVLAYPYDTMVYPKGLPAP
                     ELMWNGSAPGDKYLVHFSHALLDVKIYTLAEPPSRVTIDPALWKSIAGTVNGEQVNVR
                     IHRLKAGEVAATVVADDTWTITAEPLIGSVYYWANSLGRVLRINPGALAPEDFLLAGG
                     QNGCSTCHSVSANGKTLIVGGDIAVSTWDLVKNAPALDITTVGKPVRNWAMAAISPDG
                     TVVVENGETMLPGPPGGGDGMWDALTGQKLANTGLEGITLNMPAFSPDGTKLAYVDHT
                     SLALAVYNYDATLKQVSSPLELVIPGNDPTLNGITFPSMSPDGKWIVYHRGKYPESLD
                     TRFSTGDLYLASSEMPGVEIRLSNANGDLYPFAAGPRDLGWNYEPTFAPRTSGGYAWV
                     VFTSRRTYGNRLQGTKDEAKQLWMMAIDENPTPGVDPSHPAFWMPGQDVTTLNMRGFW
                     ALQKDDPGG"
BASE COUNT          460 a         1127 c         1003 g          484 t
ORIGIN      
        1 gtcaagaccc ccgagctcgg cggccaggcc tgcgagaccc tcgacgtctg cggcgtcacc
       61 aagcgcgcct gcgtcgccca ggacgtcggc ggcggcgtca cgctcgccag cgtcaccgcg
      121 gcgctcagcc cgacctatcc cctgttcttc tgcggtaccc ccgacctcga gccgagctgc
      181 gtgccgtccc gccccggcga gtacaccggc ctcgccaccg ccgacgaccc cgacggcgac
      241 ggcatgaccg ccgacgacaa ctgccccgac gtcttcaacc cgccgctgct gatcggcaac
      301 ggccagcagg acgccgacgg cgacatggtc ggcgacgcct gcgacacctg cccgctcgag
      361 gccggcgacg cctgcacgat gctcgacgcc gacgacatcg acgacgacgg cgtccccaac
      421 ggcagcgaca actgcccccg cgtcgccaac gccaaccagg ccgacgccga gagcgacggt
      481 cacggcgacg agtgtgacag ctgtgccgag tacaaccccg gccccggcgt gtgcccgacc
      541 acgatccccg cgatccgcaa cccgatgaac cccggccacc cggcccccgg cgcctcggtc
      601 tcgatcaccg gcgcctacgt cactgccctg cgccccgacg ccggtggcac gcgcggcttc
      661 tacgtccaga acgacacgag cgagccgtgg aacggcatct tcgtgttcac cgccagcaag
      721 ccggcgccgg tcaagctcgg caaccgcgtc gcgctcaccg gcatctacga ggagttcaac
      781 ggcctcgccg agatcaccaa cccgaccgtc acgatcgaag atccgggcct cgtgctcccc
      841 ttcgatccga tcctgctcga ccccgcgacc ctctcgaccg gcaaggccaa cgccgagccc
      901 tacgagtcga tgctcgtgca catcggcccg gtcgccatca ccgacgacaa cgccgacgac
      961 ccgatggact tcgatgagtt cgtcgtcacc ggcgccctgc gcgtcgacga ccagatcagc
     1021 gacaacctca aggacatggt cctcaacaac aagtgcgcga tcgggaccaa gttcgacgac
     1081 atcatcggcg tcctcggcta ctccttcgcc aactcgaagc tgcagccgcg cttcaaggcc
     1141 gacatcgtcc tcggcgcgat gaacatgtgc gaccccttcg ccccctgagg ggcccctcgc
     1201 gtgcccggtc cgccgggcac gcgcctacct gccgccgccg tccggcccag ggcagcctcg
     1261 ccccgcgtgc cggccgaagc gatcggcccc cgcacacgcg agtgcctgcc cgaccgcgca
     1321 cacgcgagcg cccggaccga cgactcagcc gcccggatcg tccttctgaa gcgcccagaa
     1381 cccacgcatg ttcagcgtcg tgacatcttg ccccggcatc cagaacgcgg ggtgcgaggg
     1441 atcgaccccg ggtgtcgggt tctcgtcgat cgccatcatc cacagctgtt tggcctcatc
     1501 cttggtccct tgcaggcgat tgccgtacgt acggcgcgac gtgaacacca cccacgcgta
     1561 gccgcccgac gtacgcggcg cgaaggtcgg ctcgtagttc cagccgaggt cacgcggtcc
     1621 ggcggcgaac gggtagaggt cgccgttcgc gttgctgagc cggatctcga cccctggcat
     1681 ctcgctgctg gcgaggtaga ggtcgccggt gctaaagcgg gtgtcgagcg actccgggta
     1741 tttgccgcgg tggtagacga tccacttgcc gtcgggggac atgctcggga aggtgatgcc
     1801 attgagcgtg gggtcgttgc cggggatgac cagctcgagc ggcgagctca cctgtttgag
     1861 cgtcgcgtca tagttgtaga ccgcgagcgc gaggctggtg tggtcgacgt atgcgagctt
     1921 ggtgccgtcg ggcgagaacg ccggcatgtt gagcgtgatg ccctcgaggc cggtgttggc
     1981 gagcttttgg ccggtgagcg cgtcccacat gccgtcgccg ccgccgggcg ggccgggcag
     2041 catcgtctcg ccgttctcga cgaccaccgt gccgtccggc gagatcgcgg ccatcgccca
     2101 gttgcggacc ggtttgccga ctgtagtgat atcgagggcc ggggcgttct tgaccaggtc
     2161 ccaggtcgac accgcgatgt cgccaccgac gatcagcgtc ttgccgttgg ccgacaccga
     2221 gtggcacgtg ctgcagccat tttgtccgcc ggcgagcagg aagtcctcgg gggcgagggc
     2281 gccggggttg atgcggagca cgcggccgag gctgttcgcc cagtagtata ccgagccgat
     2341 cagcggctcc gcggtgatcg tccaggtgtc gtcggcgacg accgtcgccg cgacctcgcc
     2401 ggccttgagg cgatggatcc ggacattgac ctgctcaccg ttgacggtcc ccgcgatcga
     2461 cttccacagg gccgggtcga tcgtcacgcg cgagggtggt tcggcgagcg tgtagatctt
     2521 gacatcgagc agcgcgtgcg agaagtgcac gaggtacttg tcgccggggg cgctgccgtt
     2581 ccacatcagc tccggcgcgg gcaggccctt cgggtacacc atcgtgtcgt acggataggc
     2641 gagcaccgcc tccgcgtcgg ggttgatcgc cccgtcgagc tgtagcttgt cggcctcggt
     2701 catctccggc ggggcgacga ccgccttgaa caacacgtcg atctcggcct cggcttgctg
     2761 tccctggtgc gacgcttcga tctggatctg gccgccgtag ttgcctgaca ccgtcaccag
     2821 gccgctgtcg tcgacgccgc cgaggaagct cgcctcgctg gtccacaccg cggtcaccgg
     2881 cgtgccgtcg agcttcgccg tcagttgcag cggcggggcc acgccgtcct cgagcgtgat
     2941 cgtcgccttc gcggggtcga tgacgagctg tccgccgccg ccgctgtccg acgtcgggtc
     3001 gaccgtgacg ccgccggtgc cggttggttc ggggctcccg gtcgtcggtg tcgcgccacc
     3061 cgagctcgag tccg
//
LOCUS       DASWNJ010000561         2759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_362410, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000561 DASWNJ010000000
VERSION     DASWNJ010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362410"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VGB85_09460"
     CDS             complement(<1..477)
                     /locus_tag="VGB85_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134297.1"
                     /translation="MSLRPISSLLLAVLGTVLAVAPACKSGGARSPEALRDAYAQALR
                     RDDPAAAYALLSPELQAATSRAAFTARWKAQKREREAALTAIRTLPPTHRAAVLGGRT
                     VHGDRVLTWARVAGHYQIVHGLPGVPDSSTPAQAIRAFLVAVRSADLHALEALVSDD"
     gene            complement(506..2140)
                     /gene="nadB"
                     /locus_tag="VGB85_09465"
     CDS             complement(506..2140)
                     /gene="nadB"
                     /locus_tag="VGB85_09465"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF006567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEY0134298.1"
                     /translation="MRASTPIHRSRYLVLGSGLAGLYFALEVADRGPVTILTKHNAES
                     SNTRWAQGGISAVFAGDDSSAEHVEDTLVVGAGLCDRDMVMHAVQEGPALVRRLAERY
                     GVRFDRVGGDESAPFELGREGGHSRRRVVHHRDMTGAEVERALLAAVHAHPNITVHEH
                     HTVIDLLSWSKVDGSRGCFGVYALNNRDDQVEAFVAPVTVLATGGAGRVYRYTSNPAV
                     ATGDGIAMAYRIGASVANLEFMQFHPTCLYHPDANTFLISEALRGEGGILRRGDGSDL
                     MAGRHAMGSLAPRDIVAREIDAELKRAGVRCVFLDMTHLDREYVVQRFPAIHARCMSV
                     GIDMRKDPIPVVPAAHYMCGGVVVDRFGRTDVPGLLAIGEVAMSGLHGACRLASNSLL
                     EAAVLASGAAQIADDFARGAPDQVSPWNEGAAQNSDEAVMVSANWEEARALMWNFVGI
                     VRSDKRLERGRRRIELLREEIAEYYWKFRVTRDVLELRNIALVGHLILECARLRKETR
                     GLHYNIDYPDTLPEYARNTVLDKRTGPRASDQKNPT"
     gene            2178..>2759
                     /locus_tag="VGB85_09470"
     CDS             2178..>2759
                     /locus_tag="VGB85_09470"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY0134299.1"
                     /translation="MARIGGAVWLWGVAGTATAATPSYRHYTDPQGVVHVTPATRAAE
                     PTSGAPELQGSGEGREHEVRPEGPGGPTDALPDPAATADMKIAGKARSVAAEVAEALA
                     DERSRRYETTIRAAARLYRLPESLLRAVIHTESNYNPNAVSRTNAMGLTQLMPSTARY
                     LGVTQPFEPRQNIYGGAKFLRLLANRFGGDMVLV"
BASE COUNT          408 a          880 c         1093 g          378 t
ORIGIN      
        1 gtcgtcgctg acgagggcct cgagggcgtg gaggtcggcg ctgcggactg cgacgaggaa
       61 tgcacggatc gcctgggccg gggtcgagct gtcgggcacg ccgggcaggc catggacgat
      121 ctggtagtgg ccggcgacgc gggcccaggt gagcacgcgg tcgccgtgga ccgtccggcc
      181 gcccaggacg gcggcccggt gggtcggcgg gagggtgcga atggcggtca gggccgcctc
      241 gcgctcgcgt ttctgggcct tccagcgggc cgtgaaggcc gcccggctgg tcgcggcctg
      301 gagctcgggg gacagcaggg cgtaggcggc ggccgggtcg tcgcggcgca gggcctgggc
      361 gtaggcgtcg cgcagggcct cggggctgcg ggccccaccc gatttgcagg caggggcgac
      421 agcgaggacc gtcccgagga cagcgaggag cagcgaggaa atggggcgaa gtgacactgg
      481 agccgcggta cccaagtacc ggagtttatg tggggttctt ttgatcggag gcgcgcggac
      541 cggtgcgttt gtcgagcacc gtgttgcgag catattcggg gagagtatct gggtagtcta
      601 tgttgtaatg caggccacgg gtctccttgc ggagacgggc gcactcgagg atgaggtggc
      661 cgaccagggc gatgttgcgg agctcgagca cgtcgcgggt gacgcggaac ttccagtagt
      721 actcggcgat ctcctcgcgc agcagctcga tgcggcgacg gcctcgctcg agccgcttgt
      781 cggagcggac gatgccgacg aagttccaca tgagggcgcg ggcctcctcc cagttggccg
      841 agaccatgac ggcctcgtcg gagttctggg cggccccctc gttccacggg ctgacctggt
      901 ccggggcccc gcgggcgaag tcgtcggcga tctgggcggc gcccgaggcg agcacggcgg
      961 cctcgagcag cgagttggag gcgaggcggc aggcgccgtg caggcccgac atggcgacct
     1021 cgccgatcgc cagcaggccg gggacgtcgg tccggccgaa gcggtcgacg acgacgccgc
     1081 cgcacatgta atgggcggcc gggacgaccg ggatcgggtc cttgcgcatg tcgatgccga
     1141 cggacatgca gcgggcgtgg atcgccggga agcgctggac cacgtattcg cggtcgaggt
     1201 gggtcatgtc gaggaatacg cagcgcacac cggcccgctt gagctcggcg tcgatctcgc
     1261 gggcgacgat gtcgcggggg gccagactac ccatcgcgtg acgcccggcc atcaggtccg
     1321 agccgtcgcc gcgacggagg atcccgccct caccacgcag cgcctcgctg atcaaaaacg
     1381 tattggcgtc gggatgatac aggcaggtcg ggtgaaactg catgaactcg aggttggcca
     1441 ccgaggcgcc gatccggtag gccatggcga tgccgtcccc ggtcgcgacc gcggggttgc
     1501 tggtgtagcg atagacgcgc cctgcgcctc cggtggcgag cacggtgacc ggggcgacga
     1561 aggcctcgac ctggtcgtcg cggttgttga gcgcgtacac cccgaagcag ccgcggctgc
     1621 cgtcgacctt ggaccagctg agcaggtcga tgacggtgtg gtgctcgtgg accgtgatgt
     1681 tggggtgggc atggacggcg gcgagcagcg cgcgctcgac ctcggcgccg gtcatgtccc
     1741 ggtgatgcac gacccgccgg cgcgagtgac caccctcgcg accgagctcg aagggcgcgc
     1801 tctcgtcgcc gccgacgcgg tcgaagcgga ccccgtagcg ctcggcgagc cggcggacca
     1861 gggccgggcc ctcctgcacg gcgtgcatga ccatgtcgcg gtcgcataat ccggcgccga
     1921 cgacgagggt gtcctcgacg tgctcggcgg aggagtcgtc acccgcgaac accgccgaga
     1981 tcccaccctg ggcccagcgg gtgttcgagg actcggcgtt gtgcttggtc aggatcgtga
     2041 ccggtccgcg gtcggcgacc tcgagcgcga agtagaggcc cgcgaggccg ctgccgagga
     2101 cgaggtagcg gctgcggtgg atcggggtcg aggcacgcat ggtcgcgcca ggatgctagc
     2161 atgcggccgt gcgggcgatg gcgcggatcg gcggggcggt gtggctgtgg ggggtcgccg
     2221 ggacagccac ggcggcgacc ccgagctacc ggcactacac cgacccgcag ggggtggtcc
     2281 acgtgacccc ggcgacccgg gccgccgaac cgacgagcgg ggcgccggag ctgcaaggca
     2341 gcggcgaggg tcgcgagcac gaggtccggc cggaggggcc cggcgggccg accgacgcct
     2401 tgccggatcc ggcggccacg gccgacatga agatcgccgg caaggcgcgc agcgtcgcgg
     2461 ccgaggttgc cgaggcgctg gccgacgagc gctcgcgtcg ctacgagacg accatccggg
     2521 cggccgcgcg gctgtaccgg ttgccggagt cgctgctgcg cgcggtgatc cataccgaga
     2581 gcaactacaa ccccaacgcg gtcagccgga ccaacgcgat ggggctgacg cagctgatgc
     2641 cgtcgaccgc gcgctacctc ggggtgacgc agcccttcga gccgcgccaa aacatctacg
     2701 gcggggccaa gttcctgcgc ctgctggcca atcgcttcgg cggcgacatg gtgctggtg
//
LOCUS       DASWNJ010000562         2031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_293255, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000562 DASWNJ010000000
VERSION     DASWNJ010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293255"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /locus_tag="VGB85_09475"
     CDS             complement(<1..1362)
                     /locus_tag="VGB85_09475"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY0134300.1"
                     /translation="MRARTYDPALTKAHLSNRFALSLATFFSERFGEAVTARIVEDAG
                     LSLEYLRDSERWTSVEFDRRFCDAAAHCLYGLEASPGYEHPLWQNWRDSASSMFKRQG
                     MGPLWLLLWAMQGPGGFFADIEKMYSKGNRITKMQLVDHGPGFASVRAVMTGEVADRP
                     GACWSRRGLFEAIPTIWNLPPARVEHTHCIHQDARVEHCRYDIFHEDSPAVADPYAEL
                     QRIREYVRSSIPVLLRQIDDAYLEHREAELAQRKTASYLPAHLLAEIAINPEEEVVLG
                     GRHTQGAVLFADIKGFTRRCNSVGAGEIVRQLNIYFEHMDAVILAHGGIIDKRMGDGV
                     MVVFISPDGQQDLAALGHVAVRCGLAMQRALDRCNAQLAEDGGRPLRIRVGVAAGPLV
                     QGNMGSRARLEYTVIGETVNLAARLEAAASPGHVLTLPTCVRDSDLGTARRLRVVAAK
                     GFGE"
     gene            complement(1444..>2031)
                     /locus_tag="VGB85_09480"
     CDS             complement(1444..>2031)
                     /locus_tag="VGB85_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134301.1"
                     /translation="HHAINPRYLDRNYAGILITWDRIFGSFEPEGERPVYGTTKPLRS
                     LNPLWANFHYFGEIAGLVAAAPRWRDRLYALFAHPGWRPGTSPSATPQDHDLGHHLGP
                     EALRLRASQRYDAPFRRGLGLYIAAQTLLAIPATMLLLIHAASLSAFQVLAGVALVSA
                     GAVVWAGLFERRRWAWPVEVLRVAASVALLWSIAG"
BASE COUNT          299 a          688 c          755 g          289 t
ORIGIN      
        1 ctcaccaaag cccttggccg cgaccacgcg caggcgccgg gcggtgccga ggtcgctgtc
       61 gcggacgcac gtcggcaagg tgagcacgtg gcccggcgag gcggcggcct cgaggcgcgc
      121 ggcgaggttc accgtctcgc cgatgacggt gtactcgagg cgcgcgcgcg agcccatgtt
      181 gccctgcacg agcggccccg cggcgacccc gacgcggatc cgcagcgggc ggccgccgtc
      241 ctcggcgagc tgggcgttgc agcggtcgag cgcgcgctgc atcgccaggc cgcagcgcac
      301 cgcgacgtgg ccgagcgcgg cgaggtcctg ctgcccgtcg ggcgagatga acacgaccat
      361 cacgccgtcg cccatgcgct tgtcgatgat gccgccgtgc gcgaggatca ccgcgtccat
      421 gtgctcgaag tagatgttga gctgacggac gatctcgccg gcgccgacgc tgttgcagcg
      481 gcgcgtgaag cccttgatgt cggcgaacag cacggcgccc tgggtgtgac gcccgccgag
      541 cacgacctcc tcctcggggt tgatcgcgat ctcggcgagc aggtgcgccg gcagatagct
      601 ggccgtcttg cgctgggcga gctcggcctc gcggtgctcg aggtaggcat cgtcgatctg
      661 gcgcagcagc acggggatgc tgctgcgcac gtactcgcgg atccgctgca gctcggcgta
      721 ggggtcggcg acggcgggtg agtcctcgtg aaaaatgtcg tagcggcagt gctcgacccg
      781 cgcgtcctga tgaatgcagt gcgtgtgctc gacgcgcgcc ggcggcaggt tccagatcgt
      841 cgggatcgcc tcgaacagcc cccggcggct ccagcacgcg cccggacggt cggcgacctc
      901 gccggtcatc acggcgcgca cgctggcgaa gccgggcccg tggtcgacca gctgcatctt
      961 cgtgatccgg ttgcccttgc tgtacatctt ctcgatgtcg gcgaagaacc cgccgggccc
     1021 ctgcatcgcc cacagcagca gccacagcgg gcccatgccc tgacgcttga acatgctgct
     1081 cgccgagtcg cgccaattct gccacagcgg gtgctcgtag cccggggagg cctcgaggcc
     1141 gtagaggcag tgcgcggcgg cgtcgcagaa gcggcggtcg aactcgacgg aggtccagcg
     1201 ctcggagtct cgcaggtact cgaggctcag gccggcgtcc tcgacgatgc gcgcggtgac
     1261 ggcctcgccg aagcgctcgc tgaagaaggt cgccaggctc agcgcgaagc ggttgctcag
     1321 gtgcgccttg gtgagcgctg gatcgtaggt gcgcgcgcgc atggccgacg acgtcacccc
     1381 agcgtatctg ctttgacaga agtccgcgtg ggggcctgcg cccctgggag agcggtcccg
     1441 gtgctatccg gcgatcgacc acagcagcgc gacgctggcg gcgacccgca gcacctcgac
     1501 cggccaggcc cagcgacgcc gctcgaacag gccggcccac accaccgcac cggcgctgac
     1561 cagagcgacg cccgcgagca cctggaaggc cgacagcgag gccgcgtgga tcagcaggag
     1621 catcgtcgcg gggatcgcca ggagggtctg tgcggcgatg tagaggccga gcccacgccg
     1681 gaagggcgcg tcgtaccgct ggctcgcgcg caggcgcagc gcctccggac cgaggtggtg
     1741 gccgaggtca tggtcctgcg gggtggcgct cggcgacgtg cccggccgcc acccggggtg
     1801 agcgaacagg gcgtacaggc ggtcgcgcca gcgcggcgcc gcggcgacga ggccggctat
     1861 ttcgccgaag taatgaaagt tggcccacag cgggttgagg ctgcgcagtg gtttcgtggt
     1921 cccgtacacc ggtcgctcgc cctcgggctc gaagctgccg aagatgcggt cccacgtgat
     1981 caggatgccg gcatagttgc ggtcgaggta acgcgggttg atcgcgtgat g
//
LOCUS       DASWNJ010000563         1795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_115998, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000563 DASWNJ010000000
VERSION     DASWNJ010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115998"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="VGB85_09485"
     CDS             <1..958
                     /locus_tag="VGB85_09485"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY0134302.1"
                     /translation="HDMWVGAPMQPVGPIWWAEAEVPAPDGSLYKFHDLDAEAWIADP
                     LGRRYGYDDNGQYSLVRASAAHLERWYDVAGLGLEPRDLQVYVPDGGSFTHALYVHDG
                     QNLFDPGAPFGYWKLQESVPPEMLLVGIDNTSGRIDEYTHVVDDIGDGDIGGQAGTYA
                     ELVDTIIRPRMEAAYGEAPMVGTMGSSLGGLVALVIADLYPDRYDMAISLSGTMGWGS
                     IGLNNETIIERYAAAGKRPFAIYLDSGGDGPCSDSDMDGIDDDGPSSDNYCENAQLLG
                     VLEDAGWMLGTDLWYVHSPGAPHNEGAWALRVGGAMGDFAGL"
BASE COUNT          302 a          575 c          635 g          283 t
ORIGIN      
        1 ccacgacatg tgggtcggcg cgccgatgca gccggtcggc ccgatctggt gggccgaggc
       61 cgaggtcccc gcgcccgacg gcagcctgta caagttccac gacctggacg ccgaggcctg
      121 gatcgccgac ccgctcggcc gccgctacgg ctacgacgac aacggccagt actcgctggt
      181 ccgcgcgagc gcggcccacc tcgagcgctg gtacgacgtc gcgggcctcg gcctcgagcc
      241 gcgcgacctc caggtgtacg tgccggacgg cggcagcttc acgcacgcgc tgtacgtcca
      301 cgacggccag aacctgttcg accccggggc ccccttcggc tactggaaac tgcaggagag
      361 cgtgccaccc gagatgctgc tggtcggcat tgataacact tcgggccgca tcgacgaata
      421 tacccacgtg gtcgacgaca tcggcgacgg cgacatcggc ggccaggccg ggacctacgc
      481 ggagctcgtc gataccatca ttcgcccgcg catggaggca gcctatggcg aggcccccat
      541 ggtcggcacg atgggatcgt cgctcggcgg cctggtcgcc ctcgtaattg cggaccttta
      601 ccccgaccgc tacgacatgg cgatcagcct gtcgggcacg atgggctggg ggtcgatcgg
      661 gctcaacaac gagacgatca tcgagcgcta tgcggcggcc ggaaagcggc cctttgcgat
      721 ctacctcgat tccggcggtg acgggccgtg tagcgacagc gacatggacg ggatcgacga
      781 cgacgggccc tcgagcgaca attattgtga aaatgcccag ttgctgggag tcctggagga
      841 cgccgggtgg atgctgggga ccgacctctg gtatgtgcac agtccggggg cgccgcataa
      901 cgagggggcg tgggcgctgc gggtcggcgg ggccatgggt gacttcgccg ggctgtagtg
      961 ttggtgcgcg gactggagaa cgcggggctc gggggttgtg ggcggacgac gggagggccg
     1021 cggctgcggg tccctgcgac ggcggcgcct ccccatggca cgtgctcgcc gctcggtacg
     1081 cgtcaagacc gtcactggcg gatgccccct gccgagggat tctgaccgcg acgattggtt
     1141 cgggcggctg cgcgcgagcc atgggtcccc tccacctgct tcccccgccg tcggccctct
     1201 cgtcgcccgt gtggcaccgt gggcgccccg cttgtcgccg ggcgagcact tcgtcaccga
     1261 cgcaaatcgt ggtccacgac caatcgccag tgccaacggg gccgtggcta ctcaggacgt
     1321 atatcgaggg gctctgctcg cggtatgtga ggtgaaagcc gcgtcaggcg agcacgcaga
     1381 atccgatgta actaactcgg gcggtagttg aagaccggca gggtcctgaa atggcacgca
     1441 ggtttcggaa cccttacatg gggtaggccc acgattcttg cgggcgcaag gggcgcgaat
     1501 ggccgtcggt cgtatactcg tgttcgaata tcttctccca ggagcgagtc ctttgcgatc
     1561 catgacacat cgtgaacggc cgataagcag tcgttagcga cagcatcggc gagtccaggg
     1621 gatcaaagcg cggacgataa gctgtccttc gggacagcct ctcggcacgg cgaaaagcgg
     1681 ggcgctgacg atgccacacg ggcgacgaga gggccgacgg cgggggacac aggtggaggg
     1741 gacccatggc tcgcgcgcag ccgctcgaac caatcgtcgc ggtcagaacc cctcg
//
LOCUS       DASWNJ010000564         2914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_151314, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000564 DASWNJ010000000
VERSION     DASWNJ010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151314"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VGB85_09490"
     CDS             <1..404
                     /locus_tag="VGB85_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134303.1"
                     /translation="AAPTALTLIEARYSRDDNGCCGHGDDIEIDLEGLDGAYLQEGGY
                     IEATLASGQRFVFDSDPIVVPAEADHVTLTPVSARGVRGESIEIDVSEIGGDLVYIPC
                     SIGTRTPPAALWLLAPFAWIFAHGQRRRRAV"
     gene            401..1033
                     /locus_tag="VGB85_09495"
     CDS             401..1033
                     /locus_tag="VGB85_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134304.1"
                     /translation="MIALALHLTFGTALFQEGPASEAPPAPVEPAPAPGVPEPAPAPE
                     AADPLAPAPVAEPFAVSATPDVPPKPRRFVFAFLPGVTAGVSRLPSLDFAFFFGGRLP
                     RSPWALGYQFTLSSGLAERYPEGLMTHRHHLTAMRSFGARDRGFVSIGGGVAVLAVFP
                     VVEAETRLGFRFGARRRGVVAGLARIGWNVAYREQAPMPQLGVVLGVALF"
     gene            1151..2191
                     /locus_tag="VGB85_09500"
     CDS             1151..2191
                     /locus_tag="VGB85_09500"
                     /inference="COORDINATES: protein motif:HMM:NF045039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134305.1"
                     /translation="MRDLLPIAGCLLLFTACTDSGEGQTDPAGTTTTATTTGVTTGLS
                     STGDGSTTAPTTEPTTTPTTTTAPETGTTGEPPVTTEPDTSTTVPDTSTSDTGDTSTG
                     EPVTCPPGFFCEDFEGHPEGAPPGAPWTTDIGDGSAVVESGKSVSGTRAVHFNTGNAY
                     GGRALLGLAVPEVFPTKHLYGRLRMWLTQASPDGVHWTMIEANGPTQAAGVWNDQPFA
                     ATIRYGGQHDKRLMANYDTPGFYNNNMGPGSDCWHHSQQTIPEGVWACMEWEFDSEGR
                     TMRFWLDGQEVADLTVGEMGQGCINHGTMDQWYYPDMERMSVGWVDYQDGGARELWID
                     DVTVGPARVGCE"
     gene            complement(2198..>2914)
                     /locus_tag="VGB85_09505"
     CDS             complement(2198..>2914)
                     /locus_tag="VGB85_09505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HEY0134306.1"
                     /translation="SPKTLACLPELDGVRRLIADPEVLAAWKFSSFIDTVTLDDAALA
                     DLGQTIALPSTPLLAQADDGSEDLWLIDGPAHNLRRLVPDTAVAAAWHLDLTTVLVLP
                     AVELFALAEAPPLSPRPLLVKGSGPELYVLDAELEPPEGGESSSGDSGATSVASFGND
                     DGEGDGDSGSGDGGTSSTGAGEAPPTTGAVTTSGSSSDAGPGLPEDYGVDEGCGCRSS
                     GASGAWALALLLLVPRRRRR"
BASE COUNT          419 a         1128 c          952 g          415 t
ORIGIN      
        1 ccgccgctcc gaccgcgctc acgctgatcg aggctcgcta cagccgcgac gacaacggct
       61 gctgcggcca cggtgacgac atcgagatcg atctcgaggg tctcgacggc gcatacctgc
      121 aggagggcgg ttacatcgag gccacgctcg cgagcggcca gcgcttcgtg ttcgactccg
      181 acccgatcgt cgtgcccgcg gaggctgacc acgtcacgct gacccccgtg tccgccagag
      241 gcgtacgcgg cgagtcgatc gagatcgatg tcagcgagat cgggggcgac ctggtctaca
      301 tcccgtgcag catcggcacc cgcaccccgc ccgccgcgct gtggctgctc gcgcccttcg
      361 cctggatctt cgcgcacggc cagcgccgcc ggagggccgt atgatcgccc tcgccctgca
      421 cctgaccttc gggacagctc tgtttcaaga aggccccgcc agcgaggccc cgcccgcccc
      481 cgtggagccc gcgccggccc ccggagtgcc cgagcctgcg ccggcacccg aagccgcgga
      541 cccactcgca cccgcgccag tagccgagcc gttcgccgtg tccgccacac ccgacgtacc
      601 gccgaagccc cgccgcttcg tgttcgcctt cctgcccggc gtcaccgcgg gcgtcagccg
      661 cctgccctcc ctcgacttcg cgttcttctt cggcggccgc ctgccacgca gcccgtgggc
      721 gctcggctat cagttcaccc tgtcgagcgg cctcgccgag cgctaccccg agggcctgat
      781 gacccaccgg catcacctca cggcgatgcg cagcttcggc gcccgcgacc gcggcttcgt
      841 cagcatcggc ggcggcgtgg cggtgctcgc cgtgttcccg gtcgtcgagg ccgagacgcg
      901 cctcggcttt cgcttcggcg cgcgtcgccg cggcgtcgtc gctggcctcg cgcgcatcgg
      961 ctggaacgtc gcctaccgcg agcaggcccc gatgccgcag ctcggcgtcg tgctcggcgt
     1021 cgcgctgttc tagctcgaac gccggcaccc ccgagatcga cgcgcgcata tataccgtgg
     1081 ctgcgaggcc ccgcctcgcg ccacacggcc gtcgagtccg gccgacactt gcttcgcgta
     1141 acctccggcg atgcgtgacc tcctaccgat cgccggttgc ctgctcctgt tcacggcctg
     1201 caccgacagt ggcgagggcc agaccgaccc cgcgggcacc acgaccaccg cgacgaccac
     1261 cggtgtgacc accggtctct cctcgaccgg cgacggctcg accaccgcgc cgaccaccga
     1321 accgaccacc acgccgacca ccacgactgc cccggagacc ggcacgaccg gtgagccgcc
     1381 cgtcaccacc gagcccgaca ccagcacgac cgtacccgac accagcacca gcgataccgg
     1441 cgacacctcg accggcgagc cggtgacctg tcctccgggc ttcttttgcg aggacttcga
     1501 gggtcacccc gagggcgcgc cgcccggggc tccgtggacc accgatatcg gggacggcag
     1561 cgccgtggtc gagtccggca agtccgtcag cggcacgcgt gccgtgcatt ttaatactgg
     1621 caacgcctat ggtggtcgcg ccttgctcgg gctcgcggtg cccgaggtgt tcccgaccaa
     1681 gcacctgtac gggcgcctgc gcatgtggct gacgcaggcc tcgccggacg gcgtgcactg
     1741 gacgatgatc gaggccaatg gtccgacgca ggcggccggc gtgtggaacg accagccctt
     1801 cgccgcgacg atccgctacg gcgggcagca cgacaagcgc ctgatggcca actatgatac
     1861 gcccggcttt tataacaaca acatgggccc cgggtcggat tgctggcacc attcgcagca
     1921 gacgatcccc gagggcgtgt gggcctgcat ggagtgggag ttcgacagcg agggccggac
     1981 gatgcgcttc tggctcgacg gccaggaggt cgccgacctc accgtcggcg agatgggcca
     2041 gggttgcatc aatcacggta ccatggacca gtggtattac cctgacatgg agcgcatgag
     2101 cgtcggctgg gtcgactacc aggacggcgg cgcgcgcgag ctgtggatcg acgatgtcac
     2161 cgtcggcccc gcgcgcgtcg gctgcgaata gccgcgctca gcgacgccgg cgccgtggca
     2221 ccagcagcaa cagcgccagc gcccacgccc ccgatgcccc actgctgcgg cagccgcagc
     2281 cctcgtcgac cccgtaatcc tcgggcaggc ccggacccgc gtcactgctc gacccggagg
     2341 tcgtgacggc accggtggtc ggcggcgcct cgccggcccc ggtgctgctt gtaccgccgt
     2401 ccccactccc gctgtcgcca tccccctcgc catcgtcgtt gccgaacgac gcgacgctgg
     2461 tcgctccgct gtcgcccgag ctgctctcgc cgccctcggg cggctcgagc tcggcgtcga
     2521 gcacgtacaa ctcgggcccc gagcccttca ccagcagcgg tcgcgggctc aacggcggcg
     2581 cctccgcgag cgcgaacagc tcgaccgccg gcagcacgag caccgtcgtc aggtcgaggt
     2641 gccaggccgc cgcgaccgcc gtgtccggca ccaggcgacg gagattatgc gccggtccgt
     2701 cgatcaacca aagatcctcg ctgccgtcgt cggcctgggc cagcagcggc gtggacggca
     2761 gcgcgatcgt ctgcccgagg tcggcgagcg ccgcgtcgtc gagcgtcacg gtgtcgatga
     2821 agctcgagaa cttccacgcc gcgagcacct cggggtcggc gatcaggcgc cgcacgccgt
     2881 cgagctcggg caggcacgcg agggtcttcg ggct
//
LOCUS       DASWNJ010000565         1536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_293279, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000565 DASWNJ010000000
VERSION     DASWNJ010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293279"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /gene="tsf"
                     /locus_tag="VGB85_09510"
     CDS             <1..754
                     /gene="tsf"
                     /locus_tag="VGB85_09510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEY0134307.1"
                     /translation="DCKKALTEAEGDYDKAVELLRIKGAKDVGKRLERSTTNGVVVSR
                     GGALLELDCETDFVAKNAEFVALAERLLDVVLAQKPADVDALVATEVDGQTVAALVEG
                     ESARIGEKLVLSRFAIFEGTTATYLHKRATDLPPAIGVAVQYSGGTEEAARSLAMHIA
                     AARPRFLTREEVPAEAVETERRVAEQTAKEEGKPEQAITKIVEGRVNAYYKDFVLLEQ
                     PSITEPKRTVKQIVDEAGLTVERFARFEVGQA"
     gene            854..>1536
                     /gene="pyrH"
                     /locus_tag="VGB85_09515"
     CDS             854..>1536
                     /gene="pyrH"
                     /locus_tag="VGB85_09515"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEY0134308.1"
                     /translation="MTETTAPLSAPTAFQPADGAGYKRVLLKLSGEAFGGGKVGIDSG
                     IVHSIAQQLADVVAGGTQVAVVVGGGNFFRGAELAQAGMDRRHADYMGMLGTVMNALA
                     LQAFCEQVGLETRVQTAITMGQVAEPYIPRKAVRHLEKGRLVIFGAGAGMPYFSTDTV
                     AAQRAMEIGCQVLLMAKNGVDGVYDDDPRTNPAATMYDDLDYATVLAKQLKVADATAI
                     SLSMDNRMP"
BASE COUNT          259 a          541 c          534 g          202 t
ORIGIN      
        1 ggactgcaag aaggcgctca ccgaggccga gggcgactac gacaaggccg tcgagctgct
       61 gcgcatcaag ggcgccaagg acgtcggcaa gcgcctggag cgctcgacga ccaacggcgt
      121 cgtcgtcagc cggggcggcg cgctgctcga gctcgactgc gagaccgact tcgtcgccaa
      181 gaacgccgag ttcgtcgctc tcgccgagcg cctgctcgac gtcgtcctgg cgcagaagcc
      241 ggccgacgtc gacgcgctgg tggccaccga ggtcgacggc cagaccgtcg ccgctctggt
      301 cgagggcgag tcggcccgca tcggcgagaa gctggtgctg agccggttcg cgatcttcga
      361 gggcaccacc gccacctacc tgcacaagcg cgccaccgac ctgcccccgg ccatcggtgt
      421 cgccgtgcag tacagcggtg gtaccgagga ggcggcccgc agcctggcga tgcacatcgc
      481 cgccgcgcgc ccccgcttcc tcacccgcga ggaggtcccg gccgaggcgg tcgagaccga
      541 gcgtcgcgtc gccgagcaga cggccaagga ggagggcaag cccgagcagg cgatcaccaa
      601 gatcgtcgag ggtcgggtca acgcctacta caaggacttc gtcctgctcg agcagccgtc
      661 gatcaccgag cccaagcgca cggtgaagca gatcgtcgac gaggccggcc tgaccgtgga
      721 gcgcttcgcg cgcttcgagg tcggccaggc gtaatcacgc agcatgtgaa cggctccgct
      781 cccggctccg ggagcggagc cgttcgcatg tgcggcagga tcaacccact gatgtcgaac
      841 gagggatgac gcgttgaccg agacgaccgc accactgtcc gcgcccaccg cgttccagcc
      901 cgccgacggc gccggctaca agcgggtgct gctcaagctc tcgggcgagg cgttcggcgg
      961 cgggaaggtc ggcatcgact ccggcatcgt gcacagcatc gcccagcagc tggccgacgt
     1021 cgtggccggc ggcacgcagg tcgccgtcgt cgtcgggggt ggcaacttct tccgcggcgc
     1081 ggagctcgcc caggcgggca tggaccgccg gcacgcggac tacatgggca tgctcggcac
     1141 ggtcatgaac gccctggccc tccaggcctt ctgcgagcag gtgggtctgg agacccgggt
     1201 gcagacggcg atcacgatgg gccaggtcgc cgagccctac atcccgcgca aggccgtgcg
     1261 gcacctggag aagggccggc tggtcatctt cggtgccggc gcgggcatgc cgtacttctc
     1321 gaccgacacc gtcgccgccc agcgcgcgat ggagatcggt tgccaggtgc tcctcatggc
     1381 caagaacggc gtcgacggcg tctacgacga cgacccgcgc accaaccccg cggcgacgat
     1441 gtacgacgac ctcgactacg ccaccgtgct ggccaagcag ctcaaggtcg ccgatgccac
     1501 ggcgatcagc ctgtcgatgg acaaccggat gccgat
//
LOCUS       DASWNJ010000566         3768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_645874, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000566 DASWNJ010000000
VERSION     DASWNJ010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645874"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..275)
                     /locus_tag="VGB85_09520"
     CDS             complement(84..275)
                     /locus_tag="VGB85_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134309.1"
                     /translation="MHVMRRVGGVMLASALACGGDGKGASEGSSSGDGTTGDASTTTT
                     GEPTSDTTSSPTTSTSTGK"
     gene            complement(342..914)
                     /locus_tag="VGB85_09525"
     CDS             complement(342..914)
                     /locus_tag="VGB85_09525"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0134310.1"
                     /translation="MTTAAVAGATVLTSARLLLRRMTADDLELLVRMHGDPRVMRYTG
                     GVRDRAGSEAMLRDRILDYYADHPGLGVWATIERSTGACVGIHNLNHLYGEPDIQVGY
                     LLYPEYWGRGYATEMCVALLRHGFGTVGLAKIVAITDPDNEASQHVLRKSGLVYIGER
                     SLPHPNYADGPLRWFERAAADWLAEHPDRP"
     gene            1141..3534
                     /locus_tag="VGB85_09530"
     CDS             1141..3534
                     /locus_tag="VGB85_09530"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY0134311.1"
                     /translation="MSRSIDGAADDEVHRLAADLAGKVRDLEAFARRASMLQRVTAAL
                     TRALTVREVADVVTMHSHELFGSTAALLFLFTEDGSALELHSFAGVEAGRVAPYLRMA
                     VDSDEPMSQAVRADRPVWIGTREALLAEFPHLAAPELDGRPLSGLVALPLRDTREIIG
                     GLAFSFYSDAELDPVQRDFFLTVGAQCGLAIERARSFEAERRANAQLQRQQERLALLA
                     RAAETLSSSLDSRRALVELTRLVVPTLVDWCAIDEVGPQGQIRRIAVEHRDPAKVALA
                     QQLAERYPVRPDQLHGVPAVLRTGETEWVPEIPDSILVATTHDAEHLALVRSLGLSSY
                     AVVPLIARGRVLGALSLVTEGEHRLTDEDLRFAEELARRAALALENARLYETTREHAE
                     TLTTINELGRVIAAELDQQKVVQAVTDAATTLTDAQFGAFFYNVTDERGGRYMLYTIS
                     GVARENFSKFPMPRNTAVFAPTFNGEGVVRSDDITKDPRYGHNAPRRGMPEGHLPVRS
                     YLAVPVTSRSGEVIGGIFLGHAEVAVFGERAETLAQGLAAQAAVAMDNARLYGDAQRL
                     IKALEATNRELDQFAYVTSHDLKAPLRGIGSLAEWIEEDLGDALTGDVRRKMGLLRGR
                     VARMEALIQGILDFSRAARATGKREDVDVHKLVLEIAELLAAPPPARVEVEGRLPVLN
                     TTRVSLQQVLMNLIGNALKHAGRADAQVVVSATETADFWEFRVRDNGPGIAAQYHERV
                     WGIFQTLEARDKVESTGIGLAIVRKIVEGRGGVVGIDSAAGQGATFRFTWPKRDDAR"
     gene            3547..>3768
                     /locus_tag="VGB85_09535"
     CDS             3547..>3768
                     /locus_tag="VGB85_09535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0134312.1"
                     /translation="MDPRELHILLVDDDEVDVMNVQRAFQRNRMTNPMHVASNGLEAL
                     EKLRDGSIPPRRLILLDLNMPRMNGIEFLR"
BASE COUNT          557 a         1357 c         1304 g          550 t
ORIGIN      
        1 ggcgaatatg tcatcccggc gcccggcagc gaggcggtga tcggggcgat gccggaagtc
       61 gcggccggca cggcggtgaa tttctacttc ccggtggagg tcgaggtcgt cggcgagctc
      121 gtcgtgtcgc tcgtcggttc gccggtcgtc gtcgtcgacg cgtcaccggt cgtcccgtcg
      181 ccgctcgacg acccctcgct cgcacccttg ccgtcgccac cgcaggcgag cgcgctcgcc
      241 aacatcactc caccgacccg acgcatgacg tgcatggcag gagcatcgac gccgcggcgc
      301 gccccgtcaa acggtgttca gcgggggcgt caagcctcaa gtcagggccg atccgggtgc
      361 tcggcgagcc agtccgcggc ggcgcgctcg aaccaccgca ggggaccatc cgcgtagttc
      421 ggatgcggca gcgagcgctc gccgatgtac accagcccgg acttgcgcag cacgtgctgc
      481 gaggcctcgt tgtccgggtc ggtgatcgcc acgatcttcg ccagcccgac ggtgccaaag
      541 ccatggcgca gcagcgcgac gcacatctcg gtcgcgtagc cgcggcccca gtactcgggg
      601 tagagcaggt aaccgacctg gatgtcgggc tcgccgtaca ggtgattgag gttgtggatc
      661 ccgacgcacg cgccggtcga ccgctcgatc gtcgcccaca ccccgagccc gggatgatcg
      721 gcgtagtagt cgaggatccg gtcgcgcagc atcgcctccg agcccgcgcg gtcgcgcacc
      781 ccgcccgtgt atcgcatcac ccggggatcg ccgtgcatgc gcaccagcag ctcgaggtcg
      841 tctgccgtca tgcgccgcag cagcagccgc gcgctcgtca acaccgtcgc tcctgccacc
      901 gccgcggtcg tcacggccgc agtgtatcgg gtcatcctcg cccgcggccc gcgattggtc
      961 accaggcgcg tgcgcgatcg aggccagccg ggacggtcgt ttcagccaaa ataccgcgat
     1021 cattacgggc tagtaaaatc cctgacgaca cccccctcgt cagctcgcca cgccgccacg
     1081 ctggccggcc cggtcgtttc gcgttgtgac ccgggtccat gtgagcgacc ctgtgcggcg
     1141 ttgtccagat cgatcgacgg agcggccgat gacgaggttc atcgcctcgc ggcagacctc
     1201 gcgggcaagg tccgcgacct cgaggccttc gcgcggcgag cctcgatgtt gcagcgggtg
     1261 accgcggcgc tgacccgggc gctgaccgtg cgggaggtcg ccgacgtcgt cacgatgcac
     1321 agccacgagc tgttcggttc gaccgcggcg ctgctgttcc tgttcaccga ggatggcagc
     1381 gcgctcgagc tgcactcctt tgccggcgtc gaggccggcc gcgtcgcgcc ctatctccgg
     1441 atggccgtcg actccgacga gccgatgtca caggccgtgc gcgccgatcg gccggtctgg
     1501 atcggcacgc gcgaggcgtt gctggccgag ttcccccacc tcgccgcgcc cgagctcgac
     1561 ggtcggccgc tgtcgggcct ggtggcgctg ccgctgcgag acacgcggga gatcatcggc
     1621 gggctggcct tcagcttcta cagcgacgcc gagctcgacc cggtccagcg cgacttcttc
     1681 ctgacggtcg gcgcgcagtg tggcctggcg atcgagcgcg cgcgctcgtt cgaggccgag
     1741 cgccgggcca acgcgcagct gcagcgccag caggagcgcc tggccctgct cgcgcgcgcg
     1801 gccgagacgc tgtcctcgtc gctcgactcg cgccgcgcgc tggtcgagct gacgcgcctg
     1861 gtcgtcccga cgctcgtcga ctggtgtgcg atcgacgagg tcggcccgca ggggcagatc
     1921 cgtcgcatcg ccgtcgagca ccgcgacccg gccaaggtcg ccctcgcgca gcagctcgcc
     1981 gagcgctacc cggtgcggcc cgaccagctc cacggcgtgc ccgcggtgct gcgcaccggc
     2041 gagaccgagt gggtgcccga gatcccggac tcgatcctcg tggcgaccac ccacgacgcc
     2101 gagcacctgg cgctggtgcg gtcgctcggc ctcagctcgt acgcggtcgt gccgctgatc
     2161 gcccgtggtc gggtgctcgg cgcgctgagc ctggtgaccg agggggagca ccgcctgacc
     2221 gacgaggacc tgcgcttcgc cgaggagctg gcgcgccggg ccgcgctcgc cctcgagaac
     2281 gcccgcctat acgagacgac ccgcgagcac gccgagaccc tgacgacgat caacgagctc
     2341 gggcgggtga tcgccgccga gctcgaccag caaaaggtcg tgcaggccgt caccgacgcc
     2401 gccaccacgc tgacggacgc gcagttcggg gcgttcttct acaacgtcac cgacgagcgc
     2461 ggcggacgtt acatgctcta cacgatctcg ggcgtcgctc gcgagaactt ctcgaagttc
     2521 ccgatgccgc gcaacacggc ggtgttcgcc ccgaccttca acggcgaggg tgtggtgcgc
     2581 tccgacgaca tcaccaagga cccccgctac ggccacaacg cgccgcgccg cggcatgccc
     2641 gaggggcacc tgccggtgcg cagctacctc gcggtgccgg tgacctcgcg ctcgggcgaa
     2701 gtgatcggcg gcatctttct cggtcacgcc gaggtcgccg tgttcggcga gcgggccgag
     2761 acgctcgcgc agggcctcgc ggcccaggcc gcggtggcga tggacaacgc gcgcctgtac
     2821 ggcgacgcgc agcggctgat caaggcgctc gaggcgacca accgcgagct cgaccagttc
     2881 gcgtacgtca cctcgcacga cctcaaggcc ccgctgcgcg ggatcggcag cctcgccgag
     2941 tggatcgagg aggacctcgg cgacgcgctc accggcgacg tgcgccgcaa gatgggcctg
     3001 ctgcgcggtc gagtcgcgcg catggaggcg ctgatccagg gcatcctcga cttttcgcgc
     3061 gctgcccggg cgaccggcaa gcgcgaggat gtcgatgtcc acaagctcgt cctcgagatc
     3121 gccgagctgc tcgctgcgcc gccgccggcg cgcgtcgagg tcgagggccg cttgccggtg
     3181 ctcaacacca cgcgcgtgtc gctgcagcag gtgctgatga acctgatcgg caacgcgctc
     3241 aagcacgccg ggcgggcgga cgcgcaggtc gtcgtcagcg cgacggagac cgccgatttt
     3301 tgggagttcc gggtccgcga caacggcccc ggcatcgcag cgcagtacca cgagcgcgtc
     3361 tggggcatct ttcagacgct cgaggcgcgc gacaaggtcg agagcaccgg catcggtctg
     3421 gcgatcgtca ggaagatcgt cgagggacgg ggcggcgtcg tcggcatcga ctcggcggcg
     3481 gggcaggggg ccaccttccg cttcacctgg cccaagcgcg acgacgcgag atgagcaatg
     3541 tctaagatgg acccacgcga gctacacatc ctgctggtcg acgacgacga ggtcgacgtg
     3601 atgaacgtgc aacgtgcgtt ccagcgcaac cgcatgacca acccgatgca cgtcgccagc
     3661 aacggcctcg aggcgctcga gaagctgcgc gacgggtcga tcccgccgcg caggttgatc
     3721 ctgctcgacc tcaacatgcc gcgcatgaac ggcatcgagt tcctgcgc
//
LOCUS       DASWNJ010000567         1852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_469239, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000567 DASWNJ010000000
VERSION     DASWNJ010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469239"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /gene="rplK"
                     /locus_tag="VGB85_09540"
     CDS             complement(<1..339)
                     /gene="rplK"
                     /locus_tag="VGB85_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEY0134313.1"
                     /translation="MPPKKKLAGVIKLQIKAGAATPAPPVGPALGQHGVNIMEFCKQY
                     NAATEAQRGDIVPVEISVFEDRSFTFVTKTPPAAELIKKAAGLAKGSSVPHKDKVGRL
                     TRDQVREIAEG"
     gene            complement(375..914)
                     /gene="nusG"
                     /locus_tag="VGB85_09545"
     CDS             complement(375..914)
                     /gene="nusG"
                     /locus_tag="VGB85_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEY0134314.1"
                     /translation="MSGQQGDWYVVHSYAGYENKVKTNLESRISSLNMEDYIYAIEVP
                     TEEVTEIKGGKRQQVVRKKLPGYLFVRMDLTDESWSVVRNTPGVTGFVGQTSRPSPLS
                     IDEVVKMIAPPKQKVVVAQKAVDFEVGESITVTDGPFATLPATINEINLDSQKLKVLV
                     SIFGRETPVELSFNQVAKL"
     gene            complement(1301..1570)
                     /gene="secE"
                     /locus_tag="VGB85_09550"
     CDS             complement(1301..1570)
                     /gene="secE"
                     /locus_tag="VGB85_09550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEY0134315.1"
                     /translation="MSDSAGTDTGLATPDREPSRSRRQKRPSRLRLATTFYKQVVAEL
                     RKVIWPTRRELLTYTSVVLVFVVFMVAIVSLFDFAFSQGVLAVFG"
     gene            complement(1645..1718)
                     /locus_tag="VGB85_09555"
     tRNA            complement(1645..1718)
                     /locus_tag="VGB85_09555"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1682..1684),aa:Trp,seq:cca)
BASE COUNT          242 a          616 c          681 g          313 t
ORIGIN      
        1 gccctcggcg atctcccgga cctggtcgcg cgtgagacgt ccgaccttgt ccttgtgcgg
       61 cacgctcgag cccttggcga gcccggctgc cttcttgatg agctcggcgg ccggcggggt
      121 cttggtgacg aaggtgaacg agcggtcctc gaacaccgag atctcgaccg gcacgatgtc
      181 gccgcgctgg gcctcggtgg ccgcgttgta ctgcttgcag aactccatga tgttgacgcc
      241 gtgctggccg agggcggggc ccacgggggg cgcgggcgtc gcggcgccgg ccttgatctg
      301 gagcttgatg acgccggcga gcttcttctt gggaggcatg agtcggtttc ctgggaggtg
      361 atcggcgcgg agccctagag cttcgcgacc tggttgaagg acagctcgac cggcgtctcg
      421 cggccgaaga tcgacaccag caccttgagc ttctggctgt cgaggttgat ctcgttgatc
      481 gtcgccggga gggtcgcgaa cgggccgtcg gtgacggtga tcgactcgcc gacctcgaag
      541 tcgaccgcct tctgagcgac gacgaccttc tgcttcggcg gcgcgatcat cttgacgacc
      601 tcgtcgatcg acagcggcga cgggcgcgag gtctggccga cgaagccggt gacgccgggg
      661 gtgttgcgca cgaccgacca cgactcgtcg gtgaggtcca tgcggacgaa caggtagccg
      721 ggcagcttct tgcggacgac ctgctggcgc ttgccgccct tgatctcggt gacctcctcg
      781 gtcggcacct cgatcgcgta gatgtagtcc tccatgttga gcgaggagat gcggctctcg
      841 aggttggtct tcaccttgtt ctcgtagccg gcgtaggagt gcacgacgta ccagtcgccc
      901 tgctggccgg acagctcgcg ccgcagccgc tcggacgggt cgaggtcctc gtcgtcgacc
      961 tggtcggccg cagccgcggg cagccctcgc gcgtcggcgt tcgcgtcgtg ctcggcgtcg
     1021 gccgcggcgg tgccgacccc ctcgggggcc gcgtcgtcga ggtagccgct cggggcgtcg
     1081 tcgacgccac cggcgccgag ctccgccagg tcggccgcac ccgtgcctgc ctcggtcgag
     1141 gcggcgaagc cgaccggtgc ctgcgcgacc gcctcggcct gctcggactg cacctcgtcg
     1201 cggggcagct cgaagggcgg ggtggactcg gacacgggca cgtcctgttc tgtcgtgggg
     1261 cctgtcgtac ggcggggcgg ggcgggagtg ccgggaccgg tcagccgaac accgcgagca
     1321 cgccctggct gaacgcgaag tcgaacaggc tgacgatcgc caccatgaag accacgaaca
     1381 ccagcaccac gctcgtgtag gtcagcagct cccggcgcgt gggccagatg accttgcgga
     1441 gctcggcgac gacctgcttg tagaaggtcg tcgccaggcg gagccggctc ggccgcttct
     1501 gcctgcggct gcgtgacggc tcgcggtcag gcgtggcgag accggtgtcg gtccccgcgc
     1561 tgtcgctcat cgccgtcctc tcgggagcat ctggcgccgc gaggacgtgg gcgtcccgcg
     1621 acggtcgtgc gtcggtctga cctgcagggg tgacaggagt cgaacctgcg acatccggtt
     1681 ttggagaccg gcgctctggc caactgagct acacccctcc gagggcccgt gcgacgcgag
     1741 agctccccgg cgcgatgggc gcgcctgagc agaggcgtcg agcccccaga ccgtcgagtg
     1801 tacggcttgc cgtcgtccgc tccaaccgtg ctggcgcgac ggggctcggc gg
//
LOCUS       DASWNJ010000568         2052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_151492, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000568 DASWNJ010000000
VERSION     DASWNJ010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151492"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..768
                     /locus_tag="VGB85_09560"
     CDS             82..768
                     /locus_tag="VGB85_09560"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0134316.1"
                     /translation="METTSDDALARAGLFQGVAPEAREALASALQYHDYTRGESVFTE
                     GEQGDSLFIVLTGKVKVGRRAADGRENMLSVMGPSDMFGELSLFDPGPRTATATVLTD
                     AKLASLAHAALRPWINDRPDIAEQLLRVLARRLRRTNDALADLIFTDVPGRVAKALLE
                     LAERFGTQEGEGVRVHHDLTQEELAQLVGASRETVNKALADFASRGWMRVDSRAVTIL
                     DADRLARRAR"
     gene            complement(792..>2052)
                     /locus_tag="VGB85_09565"
     CDS             complement(792..>2052)
                     /locus_tag="VGB85_09565"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0134317.1"
                     /translation="EASEHGVRAAALLVLGEVRRKRGATDAAREALVSALSAASDAGA
                     DRLTAEALRELGLLDHVDGRLRDAEERFRQAYAVAVQVDDVRGAGWALQHLAWSATTR
                     GDYALATTVLEQAAETFTALEDTGGLSWIAGTEGYVRLLQGQLVQARELAQSVLPLGE
                     AAGERWGVAVLLTIDAMAAAELGDVSTAADQAEQARQRFAELEDCWGQALALSAGGAA
                     ARGADRPDRAVELLEEAVRLADEGAYPLVGSLALVSSGYACLDRGDLDGAERAASRAT
                     ALLSGLDLEPHALLGAQVLLAQVLRARGRLEAALETVERALAGAPTPALLFPRRQALA
                     HRAGILLELGRTEQALAAAREAVAMPAQDVRAQVLAHRALGSALRAVGDQQGARSALT
                     EALRTARSTGQRSEVATTEGLLAALPG"
BASE COUNT          252 a          804 c          763 g          233 t
ORIGIN      
        1 ccagtgcgct ccgggtgacg tggaacactg tggccggaca ccgcgcctcg acgaggccag
       61 gacagcaggg gagacccggt cgtggagacc acgagcgacg acgcactggc ccgcgcgggc
      121 ctgttccagg gcgtcgcccc ggaggcccgg gaggcgctcg cctcggccct gcagtaccac
      181 gactacaccc gcggggagtc cgtcttcacg gagggtgagc agggtgactc gctgttcatc
      241 gtcctgaccg gcaaggtcaa ggtcggccgc cgcgccgcgg acggccggga gaacatgctg
      301 tcggtcatgg gcccgagcga catgttcggc gagctgtcgc tgttcgaccc cggtccgcgc
      361 acggcgacag cgacggtgct caccgacgcc aagctcgcct cgctcgccca cgcggcgctg
      421 cgaccgtgga tcaacgaccg ccccgacatc gccgagcagc tgctgcgggt gctcgcccgc
      481 aggctgcgtc ggacgaacga cgcgctcgcc gacctcatct tcaccgacgt gccgggccgc
      541 gtcgccaaag cgctgctcga gctggccgag cgcttcggca cgcaggaggg cgagggcgtg
      601 cgggtgcacc acgacctgac gcaggaggag ctcgcgcagc tcgtcggcgc ctcgcgcgag
      661 acggtcaaca aggcgctggc cgacttcgcc tcccgcggct ggatgcgcgt cgactcgcgc
      721 gccgtgacca tcctggacgc cgaccgcctg gcccgccgcg cccgctagga cgtcccggcg
      781 ctcgtctcag ctcagcccgg cagggccgcg agcagcccct cggtggtggc gacctcggag
      841 cgctgcccgg tcgatcgggc ggtgcgcagc gcctcggtga gcgcggagcg ggcgccctgc
      901 tggtcgccga cggcacgcag ggccgacccg agcgcccggt gcgccagcac ctgcgcccgc
      961 acgtcctgcg cgggcatcgc caccgcctcg cgcgctgcgg cgagggcctg ctcggtccgg
     1021 ccgagctcga gcaggatgcc ggcgcggtgg gccagcgcct ggcggcgcgg gaacagcagc
     1081 gccggcgtcg gggcgcctgc gagggcccgc tcgaccgtct ccagcgccgc ttcgagccgg
     1141 ccgcgcgccc gcaggacctg cgcgagcagc acctgggccc cgagcagggc gtgcggctcg
     1201 aggtcgagcc cggacagcag cgccgtggcg cgcgaggccg cccgctcggc gccgtcgagg
     1261 tcgccccggt cgaggcacgc gtagccgctg gacaccagcg ccagcgagcc gaccagcggg
     1321 taggcgccct cgtcggcgag gcggaccgcc tcctcgagca gctccaccgc gcggtcgggc
     1381 cggtcggcgc cgcgggctgc ggcgccgccg gcgctgagcg ccagcgcctg gccccagcag
     1441 tcctccagct cggcgaagcg ctgccgtgcc tgctcggctt ggtccgccgc cgtcgacacg
     1501 tcaccgagct cggcggcggc catcgcgtcg atggtgagca gcacggcgac gccccagcgc
     1561 tcgccagcag cctccccgag cggcagcacc gactgcgcga gctcgcgcgc ctgaacgagc
     1621 tggccctgca gcagccgcac gtagccctcc gtgcccgcga tccaggacag cccgccggtg
     1681 tcctcgagcg cggtgaacgt ctcggcggcc tgttcgagca ccgtcgtcgc gagcgcgtag
     1741 tcgccacggg tcgtggcgct ccaggcgagg tgctgcagcg cccagccggc gccgcggacg
     1801 tcgtcgacct gcaccgcgac cgcgtacgcc tgccggaacc gctcctcggc gtcgcgcagg
     1861 cgtccgtcga cgtggtcgag cagccccagc tcgcgcagcg cctcggcggt gagccggtcg
     1921 gcgcccgcgt cgctcgccgc ggacagcgcc gagacgagcg cctcccgcgc ggcgtcggtc
     1981 gccccgcgct tgcggcgcac ctcgccgagc acgaggagag cggcggcgcg tacgccgtgc
     2041 tcgctcgcct cg
//
LOCUS       DASWNJ010000569         4441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_81172, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000569 DASWNJ010000000
VERSION     DASWNJ010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81172"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..1838)
                     /locus_tag="VGB85_09570"
     CDS             complement(171..1838)
                     /locus_tag="VGB85_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134318.1"
                     /translation="MRTIALGLILCSTCADLELHADLEGPRVFASSLPRPRNVEVATM
                     PEIFVDFSEAIDPASLRVALVAWEERGSCSFTPVCAAEGTTCERGRCMTSPLTTAAVA
                     KLEEAPPEHAIPLAAPVLTDSPVGPGTRVTITPRRALQAYARHSLLVFARDLSGAPLV
                     DDDGVASVWRRDLVTAGAGSGGPEALLVTPPSGSGEVPPNLAHVATQFARPVEPDPQA
                     TLALEAEDGSRVMLVDPTPCAGWVPGLCLRWTAASPVLPDMAYRPAGGTLRDVLGLAA
                     VSPAESTWFVTASAPDLDPPQLADATLITRGPCTYAHLTASEPLQLRLTIGEGSDEAV
                     AGPGSVALALRSRQASATLRAEDLAGNASERLLQQAVPGDSLVEIPALGLAEILANPR
                     GPEPAQEFVEIVDLRGDGPALTHTDLFLADLSAADVATALAASDRPGDALPPFTTRPG
                     ERALIVAAAYDPGEGSDVAPIPGTALIRVDASLGAGGLKNAGEPVTLYHAPALGPAVI
                     VASHGNYIATEAPGHAGRSVVADPRACDLARAWASHPDGAASPGAAP"
     gene            complement(1892..2923)
                     /locus_tag="VGB85_09575"
     CDS             complement(1892..2923)
                     /locus_tag="VGB85_09575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0134319.1"
                     /translation="MTMRLGYWFPVFGGWLRNVEDEAMDTGWAYVRRLARRSEELGWD
                     LTLIAELFLNDIKGPGAPALDAWTTAAMVAAVTERLELMVAVRPTFHNPAIFAKQAAC
                     LDQLSGGRLALNVVSSWWADEARRYGVRFDAHDDRYARTAEWLAVVAGAWQEQSFSFT
                     GNHYTVEELIVAPKPARRPRPTLYAGGESEAAKRLISAACDAYVMHGDPPERVAPKIA
                     DMRRRRDALGLPDMQYGLAAYVIVRDSEAEAQRELARITDFSPGSPGYQNYQDWIAHT
                     QLEQRPSIEDYAVSNRGLRAALVGTPEVVAERIRGLAAAGVDLLLLQCSPQLEEMERI
                     AAQVFPLLR"
     gene            complement(2920..3879)
                     /locus_tag="VGB85_09580"
     CDS             complement(2920..3879)
                     /locus_tag="VGB85_09580"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134320.1"
                     /translation="MLPIAIYYEHPDWFRPLLAELARRGVPYVELDAAVHRFDPQDPA
                     PLSYAAVFNRASPSAYLRGRPQVIHYTQAWLRHLERRGVPVINGSATYALEISKADQI
                     GLLQTLGLRAPRTRVINHASQALAAAEGLRYPVLVKANVGGSGAGITRFDTARDLEDA
                     VASGLDLGIDHTALVQESAPLRDGKIHRVELLGRRFLYAIEVHPPGGSFNLCPADACQ
                     TADGRELQRSACALDAPKNGMRVAAFAPPASIIRACEAIAAAAELDVGGIEYLIDDRD
                     GEPYFYDINALSNFVADGPNVVGFDPFARLVDYLQARMDRSNR"
     gene            complement(3887..>4441)
                     /locus_tag="VGB85_09585"
     CDS             complement(3887..>4441)
                     /locus_tag="VGB85_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134321.1"
                     /translation="KTGDAHAVRYRFEQSGGRTWLILAGDPREEAAEVRYFRPEGPNH
                     LGLDLDTACALGPLTLADLADSNPPTGALGNRLWGEEVRYATGGMGFSIYQLQPQAID
                     GRGVGWHHRGDYDEWTTEALTYRQQGDRLALNFPLRHEVASTSLALWTGSDGVRRLRI
                     DDDPRDFWRPHTYRDMGPSFACTP"
BASE COUNT          588 a         1528 c         1708 g          617 t
ORIGIN      
        1 tagacggtgc tgacgacctt gccctccagg cgctgctcgg cagggtcgcc tgtgcgtggc
       61 tcggagggtt tcatctcttc ggggcggtcc cacaagacat atcgccgatc tggtggtggc
      121 gctcaagggc ccgcgagatc gcgggcagat catgtacagg cgagcatgcg tcacggcgcc
      181 gcgcccggcg aggccgcgcc gtccgggtgc gaggcccagg cgcgcgccag atcgcaggcc
      241 cttggatcgg cgacgacgct gcggccggcg tggccgggcg cctcggtggc gatgtaattt
      301 ccgtggctgg cgacgatcac cgccgggccg agcgcgggcg cgtgatacag cgtgaccggc
      361 tcgcccgcgt tcttgagccc gcccgcgccg agcgaggcgt cgacgcggat cagcgcggtg
      421 ccggggatcg gggcgacatc gctgccctcg ccggggtcgt acgcggccgc gacgatcagc
      481 gcgcgctcgc cggggcgggt cgtgaacggc ggtaatgcat cgcccgggcg atcgctcgcc
      541 gcgagcgccg tcgcgacatc ggccgcggac aggtccgcga ggaacaggtc ggtgtgcgtg
      601 agcgcggggc cgtcgccgcg caggtcgacg atctcgacga attcctgcgc cggctccggg
      661 ccgcgtgggt tggcgaggat ctcggcgagg ccgagcgccg gtatctcgac caggctgtcc
      721 cccgggacag cttgttgcaa aaggcgctcg ctggcgttgc cggcgaggtc ctcggcgcgc
      781 aacgtcgccg atgcttgtcg gctgcgcagc gcgagcgcga ccgagcccgg gccggcgacg
      841 gcctcgtcgc tgccctcgcc gatcgtcagg cgcagctgca acggctcgct ggcggtgaga
      901 tgcgcgtagg tgcacgggcc gcgcgtgatg agggtcgcgt cggcgagctg cggcgggtcg
      961 aggtccggcg cggacgcggt gacgaaccat gtcgactccg cgggggacac cgcggcgagc
     1021 ccgaggacgt cgcgcagcgt gccgccggca ggccggtagg ccatgtccgg cagcacgggg
     1081 ctcgccgcgg tccagcgcag gcacaggccc gggacccagc cggcgcacgg tgtcgggtcg
     1141 acgagcatca cgcggctgcc gtcctcggcc tcgagcgcga gcgtggcctg cgggtcgggc
     1201 tcgacggggc gggcgaactg cgtggcgacg tgcgcgaggt tcggcggcac ctcaccggag
     1261 ccggacggcg gcgtgaccag cagcgcctcc gggccgcccg agcccgcgcc cgcggtcacc
     1321 aggtcgcgcc gccacacgct cgccacgcca tcatcgtcga ccaacggcgc gccgctgagg
     1381 tcgcgggcga acaccagcag gctgtgccgc gcgtacgcct gcagggcccg ccgcggcgtg
     1441 atcgtcactc gtgtgcccgg gccgaccggg ctgtccgtga ggacaggtgc tgcgagcggg
     1501 atcgcgtgct ccggcggcgc ctcctcgagc tttgcgaccg ccgccgtggt cagcgggctc
     1561 gtcatgcacc ggccgcgctc gcaggtcgtg ccctcggccg cgcacacggg ggtgaagctg
     1621 cacgacccgc gctcctccca ggcgaccagc gcgacccgca gcgaggccgg gtcgatcgcc
     1681 tcggaaaaat cgacgaagat ctccggcatc gtcgcgacct cgacgttgcg cggccgcggc
     1741 agcgagctgg cgaacacgcg gggaccctcg aggtcggcgt gcagctcgag gtcggcgcag
     1801 gtggagcaca ggatcaggcc cagggcgatc gtacgcatgg gagcgagctc ggcccctgcc
     1861 gcgccgtcgt tgcgcctgca acgtgctttt ttcagcgcag cagcgggaac acctgggccg
     1921 cgatgcgctc catctcctcg agctgcgggc tgcactgcag cagcagcagg tcgacgccgg
     1981 cggccgcgag cccgcggatg cgctccgcga cgacctccgg ggttcccacc agcgccgccc
     2041 gcagaccgcg gttgctgacc gcgtagtcct cgatgctcgg gcgctgctcg agctgcgtgt
     2101 gggcgatcca gtcctggtag ttctggtagc cgggcgagcc gggcgagaag tcggtgatgc
     2161 gggccagctc gcgctgcgcc tcggcctcgc tgtcgcgcac gatcacgtag gccgcgaggc
     2221 cgtattgcat gtcgggcagg ccgagcgcgt cccggcgacg gcgcatgtcg gcgatcttcg
     2281 gggcgacgcg ctccggtgga tcgccatgca tgacgtaggc gtcgcaggcg gcggagatca
     2341 ggcgcttggc ggcctccgac tcgccgccgg cgtagagcgt cggccggggc cggcgcgccg
     2401 gcttcggggc gacgatcagc tcctcgaccg tgtagtgatt cccggtgaac gagaagctct
     2461 gctcctgcca ggcgccagcg acgaccgcga gccattcggc ggtgcgggcg tagcggtcgt
     2521 catgggcgtc gaagcgcacg ccgtagcggc gcgcctcgtc ggcccaccac gaggacacga
     2581 cgttgagcgc gagccgcccg ccgctgagct ggtcgaggca cgcggcctgc ttggcgaaga
     2641 tcgccgggtt atgaaaagtc gggcgcaccg cgaccatcag ttcgaggcgc tcggtgaccg
     2701 cggcgaccat cgccgcggtc gtccaggcgt cgagcgcggg cgccccggga cccttgatgt
     2761 cgttgaggaa cagctcggcg atcagcgtca ggtcccagcc gagctcctcg ctgcggcggg
     2821 ccagccggcg cacgtacgcc cagccggtgt ccatggcctc gtcctcgacg ttgcgtaacc
     2881 agccgccgaa caccgggaac cagtagccga ggcgcatcgt catcggttgc tgcgatccat
     2941 gcgcgcctgg agatagtcga cgaggcgcgc gaaggggtcg aagccgacca cgttcgggcc
     3001 gtcggcgacg aagttcgaca gcgcgttgat gtcgtagaag tacggctcgc cgtcgcggtc
     3061 gtcgatcagg tactcgatgc cgccgacatc gagctcggcc gccgccgcga tcgcctcgca
     3121 ggcgcggatg atgctcgcgg gcggggcgaa ggcggccacg cgcatgccgt tcttcggggc
     3181 gtcgagggca cacgcggacc gttgcagctc gcggccgtcc gcggtctggc aggcgtccgc
     3241 cgggcacagg ttgaagctcc cgccgggcgg gtggacctcg atcgcgtaca ggaagcgccg
     3301 gccgagcagc tcgacgcggt gaatttttcc atcgcgcagc ggcgcgctct cctggaccag
     3361 cgcggtgtga tcgatgccga gatcgaggcc gctggcgacc gcgtcctcga ggtcccgcgc
     3421 cgtgtcgaag cgggtgatgc cggccccgct gccgccgacg ttggccttga cgagcaccgg
     3481 gtagcgcagg ccctcggcgg cggcgagcgc ctggctggcg tggttgatca cgcgggtccg
     3541 cggggcccgc agccccaggg tctgcagcag gccgatctgg tcggccttcg agatctcgag
     3601 ggcataggtg gcgctgccgt tgatcaccgg cacgccgcgg cgctcgaggt gacgcagcca
     3661 cgcctgcgtg tagtggatca cctgcgggcg gccgcgcagg tacgccgagg ggctcgcgcg
     3721 gttgaacacg gccgcgtagg acagcggcgc cgggtcctgc gggtcgaagc ggtggaccgc
     3781 ggcgtcgagc tcgacgtacg ggaccccgcg gcgcgcgagc tcggccagca gcggacgaaa
     3841 ccagtccggg tgctcgtagt agatggcgat cggcagcatg gctttatcag ggcgtgcagg
     3901 cgaacgaggg gcccatgtcg cggtaggtgt gcgggcgcca gaagtcgcgc gggtcgtcgt
     3961 cgatgcgcag gcgccggacc ccgtcgctcc cggtccacag cgcgaggctg gtgctcgcga
     4021 cctcgtgacg cagcgggaag ttgagcgcga ggcggtcgcc ctgctggcgg taggtcagcg
     4081 cctcggtggt ccactcgtcg tagtcgccgc ggtgatgcca gccgaccccg cgcccgtcga
     4141 tcgcctgcgg ctgcaattga tagatggaga agcccatgcc gccggtcgcg tagcggacct
     4201 cctcgcccca caggcgattg ccgagcgcgc cggtcggcgg gttgctgtcc gcgagatcgg
     4261 cgagcgtcag cgggccgaga gcgcaggcgg tgtcgaggtc aaggccgagg tggttcggcc
     4321 cctcgggcct gaagtagcgc acctccgcgg cctcctcgcg cgggtcaccg gcgaggatca
     4381 gccacgtgcg gccgcccgac tgctcgaagc ggtagcggac cgcgtgcgcg tcgccggtct
     4441 t
//
LOCUS       DASWNJ010000570         2524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_469284, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000570 DASWNJ010000000
VERSION     DASWNJ010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469284"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VGB85_09590"
     CDS             <1..381
                     /locus_tag="VGB85_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134322.1"
                     /translation="TLGERDFLAFVHHGEERARRQFEAYSTWFKGQARDLDHKLFETI
                     LVDERRHGAYTRDYLVALVGEPEARRAIRRVARWELGRRWMRAGRFVAERVYIATMLL
                     LYLLAAPLALLVRLARPIHRGWQR"
     gene            388..2097
                     /locus_tag="VGB85_09595"
     CDS             388..2097
                     /locus_tag="VGB85_09595"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0134323.1"
                     /translation="MACVLGISAYYHDAAAALVVDGEVVAAIQEERLSRIKNDPGLPI
                     SAALACLAYAGIDPAAVDALAFYEEPFIKLERVMVHLLRHLPRTWKMFPRAIAGQLGA
                     KIWVLDQLAESLGVARDRVVSFEHHRSHAASAYLVSPYDSAAVLVVDGVGEVHSTSIW
                     DGHQGELRMLASIDFPHSLGLLYAGLTAWLGFAVNEGEYKVMGLAAWGRPTLRAAFDR
                     VVHLHADGSYELELACFAHMSDAELGFGPALERVLGPRRPPGKPWDLTDPADLRYADA
                     AATLQSVLEDALLGLARRARELTGRRDLCLAGGVALNALANARLRREAGFDRVFVQPA
                     AGDAGGALGAALLASHRLGDRRPTSMSSAALGLPIDVARTHAVAGELGLRVRRVDDPA
                     ALTAELIADDRIVAHARGRFEWGPRALGQRSILASPRDRATRDRLNRVIKQREPFRPF
                     APAILSERTADFFTAGADDMSPFMTTVAPVRPEARARLAAVTHEDGTARLQTVEAARA
                     PELHAVLAALAAAGHDPVVLNTSLNGAGEPIVASGEDALAFLLRHAVDALIVEDLVIE
                     RPA"
     gene            2094..2303
                     /locus_tag="VGB85_09600"
     CDS             2094..2303
                     /locus_tag="VGB85_09600"
                     /inference="COORDINATES: protein motif:HMM:NF039953.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HEY0134324.1"
                     /translation="MTPAPRPTRPGLARRLAARARTLARLIGHFLHPARFILAPLLVV
                     LLLAGILLVLTGGLAYVAPFVYTMF"
BASE COUNT          301 a         1054 c          772 g          397 t
ORIGIN      
        1 accctcggcg agcgcgactt cctcgccttc gtgcaccacg gggaggagcg cgcccgccgg
       61 cagttcgagg cctactcgac ctggttcaag ggccaggccc gcgacctcga ccacaagctg
      121 ttcgagacga tcctcgtcga cgagcgccgc cacggcgcct acacccgcga ttatctcgtc
      181 gcgctcgtcg gcgagccgga ggcccgcagg gcgatccgcc gcgtcgctcg ctgggagctc
      241 gggcgccggt ggatgcgggc cggtcgcttc gtcgccgagc gtgtctacat cgccaccatg
      301 ctgctgctgt acctgctcgc cgccccgctg gcgctgctcg tccgcctggc gcgaccgatc
      361 caccgcggct ggcagaggtg accacgcatg gcctgcgtgc tcggcatctc cgcctattat
      421 cacgacgccg ccgccgcgct cgtcgtcgac ggtgaggtcg tcgccgcgat ccaggaggag
      481 cggctgtcac ggatcaagaa cgaccccggc ctgccgatct ccgccgcgct cgcctgcctc
      541 gcgtacgccg gcatcgaccc cgccgccgtc gacgccctcg ccttctacga ggaaccattt
      601 attaaactcg agcgcgtcat ggtgcacctg ctgcgccact tgccccggac ctggaagatg
      661 ttcccgcggg cgatcgccgg ccagctcggc gccaagatct gggtcctgga tcagctcgcc
      721 gagagcctcg gtgttgcgcg cgaccgcgtc gtcagcttcg agcatcaccg cagccacgcc
      781 gccagcgcgt acctcgtctc cccgtatgac agcgccgccg tgctcgtcgt cgacggcgtc
      841 ggcgaggtcc actcgacctc gatctgggac ggccatcagg gtgagttacg catgctcgcc
      901 agcatcgact tcccgcactc gctcggcctg ctctacgccg gcctgaccgc ctggctcggc
      961 ttcgccgtca acgagggcga gtacaaggtc atgggcctcg ccgcctgggg ccgaccgacc
     1021 ctgcgcgccg cgttcgaccg cgtcgtccac ctccacgccg atggctccta cgagctcgag
     1081 ctcgcctgct tcgcccacat gagcgacgcc gagctcggct ttggccccgc gctcgagcgc
     1141 gtgctcggcc cgcgccgccc gcccggcaaa ccgtgggacc tcaccgaccc cgccgacctg
     1201 cgctacgccg acgccgccgc gacgctccag agcgtgctgg aggacgccct gctcggcctt
     1261 gcccgccgcg cccgcgagct gaccggccgt cgcgacctct gcctcgccgg cggcgtcgcc
     1321 ctcaacgccc tcgccaacgc ccgcttgcgc cgcgaggccg gcttcgaccg cgtcttcgtg
     1381 caacccgctg cgggtgacgc cggaggcgct ctcggggccg ccctgctcgc ctcgcaccgc
     1441 ctcggcgatc gccggccgac ctcgatgtcg agcgccgcgc tcggtctccc gatcgacgtc
     1501 gcccgcaccc acgccgtcgc cggcgagctc ggcctccgcg tgcgccgcgt cgacgacccc
     1561 gccgcgctga cggccgagct gatcgccgac gaccgcatcg tcgcgcacgc ccgcggccgc
     1621 ttcgagtggg gcccccgcgc gctcggccag agatcgatcc tcgcctcccc gcgcgaccgg
     1681 gccacgcgcg accggctcaa ccgcgtcatc aaacagcgcg agccgtttcg tcccttcgcc
     1741 cctgcgatcc tgtccgaacg gacagccgac ttttttaccg ccggggccga cgacatgagc
     1801 ccgttcatga ccaccgtcgc ccctgtgcgc cccgaggccc gggcccgcct cgcggcagtc
     1861 acccacgagg acggcaccgc gcgcctccag accgtcgagg ccgcccgcgc ccccgagctg
     1921 cacgccgtgc ttgcggcgct ggccgccgcg ggccacgatc cggtcgtgct caacacctcg
     1981 ctcaacggcg ccggcgagcc gatcgtcgcc agcggcgagg acgccctggc cttcttgctg
     2041 cgccacgccg tcgacgcttt gatcgtcgag gacctcgtga tcgagcggcc cgcatgaccc
     2101 cagccccacg ccccacgcgc cccggccttg cgcgccgcct cgccgcccgc gcccgcaccc
     2161 tcgcgcgcct gatcggccac ttcctgcacc ccgcgcgttt catcctcgcg ccgctgttgg
     2221 tcgtgctcct gctcgccggg atcctgctcg tgctgaccgg cggcctcgcc tacgtcgcgc
     2281 ccttcgtcta tacgatgttc tgatcgccgt ggcctgcggc ccccgatctt gcattgtgta
     2341 catatcgcgg ccgcgcatcg catcgctcgt tcgccctgcg cgacgatctc cgcgcgacag
     2401 cctccggccg ccgtgatatc gcatttcatc acctgtttta tgcagcgaca tcacctcgtc
     2461 gtccccgccc tcctgttgtt cgcatgctcc gaccgccccg cggtcgagac cgcggactcg
     2521 accg
//
LOCUS       DASWNJ010000571         1715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_646112, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000571 DASWNJ010000000
VERSION     DASWNJ010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646112"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VGB85_09605"
     CDS             <1..747
                     /locus_tag="VGB85_09605"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEY0134325.1"
                     /translation="AVRGGLLSRRALLATAGPLLLVLAATPSWPAFAAPGGARRQSLR
                     YQIEVAGVLVGGVDFTAEAGDEAVDARVRWRVDGLLGLLDREKGRMEARGRVDGDGRV
                     VPASFASLFEKPDRKREVSIRYGRDGDIRELELTRDGRSRESEVPERLRDATVDTITA
                     FWRLREWLDASGGRDEDGDGGAEERVELTLVPRSGFDDGDKVFGSRLDPDEPWAELLV
                     AVGERDGAVPISLAGRGRMKWRITLDGEGE"
     gene            complement(866..1207)
                     /gene="grxD"
                     /locus_tag="VGB85_09610"
     CDS             complement(866..1207)
                     /gene="grxD"
                     /locus_tag="VGB85_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEY0134326.1"
                     /translation="MSDQQVNDRIKQQIGGDDVVLYMKGTPVFPMCGFSAAVVQVLSR
                     EGVKFQSYNILEDQELRQGLKAFSDWPTFPQLYVKGELVGGCDIVREMHESGELEGFL
                     KEKGVKAGVAA"
     gene            complement(1279..1512)
                     /locus_tag="VGB85_09615"
     CDS             complement(1279..1512)
                     /locus_tag="VGB85_09615"
                     /inference="COORDINATES: protein motif:HMM:NF013851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEY0134327.1"
                     /translation="MPMSAVEIERLILEFLPDARVAIEDLAGDGDHYRATIVAEAFRG
                     KSRLQQHQLVYKALGERMGGELHALALSTSAPG"
     gene            complement(1526..>1715)
                     /locus_tag="VGB85_09620"
     CDS             complement(1526..>1715)
                     /locus_tag="VGB85_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134328.1"
                     /translation="AVEAGRADALIAAASAAGVTAREVGRTGGDALHIPGEAPISLAE
                     MGRRREAWLPAYMAGGPG"
BASE COUNT          233 a          599 c          638 g          245 t
ORIGIN      
        1 gccgtgcgcg gcggcctcct ctcgcgccga gccctgctgg cgactgcggg cccgttactc
       61 ctggtactcg cggccacccc gtcgtggccc gcgttcgcgg cgcccggcgg cgcgcggcga
      121 cagagcctgc gctatcagat cgaggtcgcg ggcgtcctgg tcggaggggt ggatttcacg
      181 gccgaagccg gcgacgaggc ggtcgacgcc cgggtccggt ggcgggtgga cgggctgctc
      241 gggctgctcg accgcgagaa gggcaggatg gaggcccgag gccgggtcga cggcgacggg
      301 cgggtggtgc cggccagctt cgccagcctg ttcgagaagc ccgaccgcaa gcgcgaggtc
      361 agcatccgct acgggcgcga cggcgacatc cgcgagctgg agctgacccg cgacggccgc
      421 agccgcgaga gcgaggtgcc cgaacggctc cgggacgcca cggtcgacac gatcaccgct
      481 ttctggcggc tgcgcgagtg gctggatgct agtggcggtc gcgacgagga cggcgacggc
      541 ggcgccgagg agcgggtcga gctgacgctg gtgccgcgct ccggcttcga cgacggcgac
      601 aaggtgttcg gcagccgcct cgacccggac gagccctggg ccgagctgct ggtcgcggtc
      661 ggcgagaggg acggcgcggt gccgatcagc ctcgccgggc gcgggcggat gaagtggcgg
      721 atcaccctgg acggggaagg ggagtagccg aacgccggca cgacctcgcc ctctcaccga
      781 ggctacggcc cctggtcctg tcaccccggg tcgagcccgg ggtgacgata ggggaggttg
      841 aaggggcttt agccgctggc gccgctcagg ccgcgacccc cgccttgacg cccttctcct
      901 tcaggaagcc ctcgagctcg cccgactcgt gcatctcgcg gacgatgtcg cagccgccga
      961 ccagctcgcc cttgacgtag agctggggga aggtcggcca gtcgctgaac gccttcaggc
     1021 cctggcgcag ctcctggtcc tcgaggatgt tgtaggactg gaacttgacg ccctcgcgcg
     1081 agagcacctg gacgacggcg gcggagaagc cgcacatcgg gaacaccggg gtgcccttca
     1141 tgtagaggac gacgtcgtcg cccccgatct gctgcttgat gcggtcgttg acctgctggt
     1201 cgctcattgg cctcgctcct actactagcc ttgacctact tcttgccgac tcacggtcgc
     1261 gccgggcgac gccgagtatt atcccggggc cgacgtggac aatgccagcg cgtgcagctc
     1321 gccgcccatg cgctcgccga gcgctttgta gaccagctgg tgctgctgga ggcgtgactt
     1381 gccccggaac gcctcggcca cgatcgtggc ccggtagtgg tcgccgtcgc cggcgagatc
     1441 ctcgatcgcg acccgcgcgt cggggaggaa ctccaggatc agccgctcga tctcgacggc
     1501 gctcatcggc atcgacgaaa gtctcctagc ccggcccgcc cgccatgtag gcgggcagcc
     1561 acgcctcgcg gcggcgcccc atctcggcca gggatatggg cgcctcgcca gggatgtgca
     1621 acgcgtcgcc cccggtgcgg ccgacctcgc gcgcggtcac gcccgccgcg gaggccgcgg
     1681 cgatcagggc gtcggcgcgg ccggcctcga ccgcg
//
LOCUS       DASWNJ010000572         6212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_434905, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000572 DASWNJ010000000
VERSION     DASWNJ010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6212
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434905"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VGB85_09625"
     CDS             <1..604
                     /locus_tag="VGB85_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134329.1"
                     /translation="MAGVAYLVKRSEQRDLQALEQEIRERRAAIEQENSARQRLERFI
                     EDTVETAAARHFGQARDRIEAGERGRDLRARSREQERDTRATDAERRRDAKSVERDRQ
                     AAEAERERDRRAVEAGAERDRRSAAAEASRAGSAASAWAQQQRELQQRADAQLAAYDA
                     KMQEFKEQLMALARPPTPAPSPGAAPGSVTDRLRKLYDDD"
     gene            591..917
                     /locus_tag="VGB85_09630"
     CDS             591..917
                     /locus_tag="VGB85_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134330.1"
                     /translation="MTTTDEKIDELARQVEAMTVALERLYALLSERPAGGGKRRAKPS
                     PAQLAELQKTTAYKARVARAAKRYGVPLAEWLERFGPVDKLPPGVPRPAPSLPVTPYR
                     RTVTRP"
     gene            1035..1427
                     /locus_tag="VGB85_09635"
     CDS             1035..1427
                     /locus_tag="VGB85_09635"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEY0134331.1"
                     /translation="MRASPLGALEAAVMEHLWEQGPGDVKRVHAALKARRITLNTVQS
                     TLRRLFDKQLLRREKISHAHVYSPRLTRPEFQRQVLADVVGDVLRGEASAALSAFVDL
                     AAEVDEAHLRTLEQLIATRLQARSGGAT"
     gene            1424..2413
                     /locus_tag="VGB85_09640"
     CDS             1424..2413
                     /locus_tag="VGB85_09640"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEY0134332.1"
                     /translation="MTSGSPLVLWLGFAGVLGAATMVVAIALGLPRLGPALERLAPGR
                     RARVLTALCLAPLGTGLALALACLLPSLYAAVWPGLDHCTRHADEHLHLCLVHPPSAV
                     VGTAAVLVGATCLLFVLLRAAPGVVDLLRARRVLARLREVSAETVLAGVRSVDSDAPF
                     SFTGGLLAPDIYLSRGLRERLEAPQLAAVLAHERAHVQRRDVLRRHVAQLGALLHAPG
                     TARSLVSALDLACEEACDAEAAAIIGDRARVAEAIVAVARLGPRPPPALAASVVGFTA
                     DALTHRVNALLSTPPPESKAPWWTLAGGFLALVVLAPALHHATETVLSFLAHP"
     gene            2419..3696
                     /locus_tag="VGB85_09645"
     CDS             2419..3696
                     /locus_tag="VGB85_09645"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY0134333.1"
                     /translation="MPLRLHSITDKKPRAYRCSALLGFVLTLLIRTGAQAAPTTAVDE
                     PTVVRRALARNAVSDIIEGDIEAARATAVELRTWQNPELAYTREQTYGTAGTGEDYLW
                     LAQTFDIGGRRSLRGRAAMLRSEGAAARGESLKVLLAAEVRLRYHEVLYRRLRVEALT
                     LWSQRLSTALTAIAAREKAGDVARYDRRRLDRELALARAREEVEATALVKAWARLAAL
                     AELDAAAPQSVPGALLPATRPEAVDQYLARAATRPDIRALERAEAATELDYKAAARAR
                     VPELTLGAGWKGVQYTGLRSDGFVVNASMTLPVVDRGQGPRRRAEAEGLALRGRASLA
                     RQELAGEILGLRAEADRLHVLADQFTRERRRDADDLMRIADAAYRGGELGILELLDAF
                     RGAVDDELQGLELALMARRARIELERAAGGGLP"
     gene            3693..5243
                     /locus_tag="VGB85_09650"
     CDS             3693..5243
                     /locus_tag="VGB85_09650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0134334.1"
                     /translation="MKHALALLLCLLIPGCTRDVAEEHGDHAHPHAEEDPRPGLVFTK
                     WDAHSELFVEFPALVVGQESRFAAHLTRLSDFGAVDSGTMTAELGDGAEALRAVADAP
                     SRPGIFRPTLTPTRPGTFRLVLRYESPGLAGAHDLGEITVYPDTNAAVAAAPPGADLG
                     GVPFLKEQQWQIPFATEPAAARSLRPSMAVYATLRARPDGEVSIHAPVAGRLGAGTGR
                     FPQMGARITKDEVLAAIVPQLGQDSDRATLDLAVQQARIDVDNARIERERLESLLKAG
                     AIPERRVIEARHSEREALALQTAASRRRGTFGRVQRTGGGAGDIAVRAPLSGTVVDVQ
                     TTPGSFVAAGQALLVAADLGRLWLEAHVPEHQASGLGKPAGAWFRVPGIDEVVEVGAE
                     GLVTVAGRIDPETRTLPVVFAIDNPGERLPIGTFVEAHLVTGPPREALAVPTAAVLTE
                     AGQAVVYVQPEGERFERRPVELGVQDGAWIEVVRGVAAGERVVSRGAYLVRLAGASGT
                     VPTHGHAH"
     gene            5257..>6212
                     /locus_tag="VGB85_09655"
     CDS             5257..>6212
                     /locus_tag="VGB85_09655"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0134335.1"
                     /translation="MIDAVIRWSLQNRLLVLLAALALVVWGSTTVRRMPVDVFPDLTA
                     PTVTVLAEAHGMAPQELEVQVTFPIEAALNGAAGVRRVRSSTAVGIAVIWVEFEWGQD
                     PYRARQIVSERLQLVRDALPPEVEPPILAPMSSIMGEIMFVALTGEQHDPMDLRTQAD
                     WTLRRRLLAVPGVSQVIVLGGDVRQYQVRVDPARLVAHGLGLEDVTAAVGATNANSSA
                     GFLIEGGQESIIHGIGRVSRPEDIADTVLVAHQTAPVRVGDVATVALGPALKRGDGSY
                     NARPAVVIGVQKQPGVNSLELTARIDELLTDLQPTLPSGMQI"
BASE COUNT          842 a         2312 c         2184 g          874 t
ORIGIN      
        1 aatggcgggc gtcgcctacc tcgtgaaacg ttcggagcag cgcgacctgc aggccctcga
       61 gcaagagatt cgcgagcgga gggcggccat cgagcaagag aacagcgcgc ggcagcgact
      121 cgagcgcttc atcgaggaca ccgtcgagac cgcggcagcg cgccacttcg ggcaggcacg
      181 tgaccgcatc gaggccggcg aacgaggccg cgacctgcgg gccaggtcgc gcgagcagga
      241 gcgggacacg cgggcgaccg acgccgaacg gcgacgcgac gcaaaatccg tcgagcgcga
      301 ccgccaggcc gcagaagcgg agcgcgagcg ggaccgccgg gccgtcgagg ccggggcgga
      361 gcgcgatcga agatctgccg ccgccgaggc gtcgcgcgca ggttcggccg cgagcgcgtg
      421 ggcgcagcag cagcgcgagc tccaacaacg ggcggacgcc cagctcgccg cgtacgacgc
      481 gaagatgcag gagttcaagg agcagctgat ggccctggcg cggccgccga cgccggctcc
      541 atcgcccggg gcggcgcccg gcagcgtcac cgaccggctt cggaaactgt atgacgacga
      601 ctgacgaaaa aatcgacgag ctcgcccgac aggtcgaggc gatgaccgtc gccctggaac
      661 gcctttacgc cctgctcagc gagcggcctg ccggcggcgg caaacgccgg gcgaagccgt
      721 cgcccgccca actcgccgag ctacaaaaga ccacggcgta caaggcccgc gtggcacggg
      781 ccgcgaagcg ctacggagtg cccctcgccg agtggctcga gcgcttcggg cccgtcgaca
      841 agctcccgcc cggtgtgcca cggcccgccc cgagcttgcc ggtgacgccc taccgacgga
      901 cagttacccg accttaaaac gccatacctg ccgcccttgc gcacgaggat ctgggtgtat
      961 ctggacaggc tcgggtccga gagcgtccct aaaaccgatg gacgctcgtt ttttgcgcgt
     1021 ggtagcgtct cgccgtgcga gcaagcccgc tcggagccct cgaggccgcc gtcatggagc
     1081 acctgtggga gcaggggccc ggcgacgtca agcgcgtcca cgcggcgctc aaggcccgca
     1141 ggatcacgct caacacggtc cagtccaccc tgcggcggct gttcgacaag caactcctgc
     1201 ggcgcgagaa gatcagccac gcgcacgtct acagcccgcg gctgacgcgg ccggaattcc
     1261 agcgccaggt gctcgccgac gtcgtcggtg acgtgctgcg gggagaagca tcggcggcgc
     1321 tgtcggcctt cgttgacctc gccgccgagg tcgacgaggc ccacctgcgc acgctcgagc
     1381 agttgatcgc gacccggctg caagctcgct ccggaggtgc cacgtgacgt ccggctcgcc
     1441 gttggtcctc tggcttggat tcgcgggggt gcttggcgcg gcgaccatgg tcgtggcgat
     1501 cgcacttggt ttgccccggc tcgggccggc gctcgagcgt ctcgctccgg gtcggcgcgc
     1561 tcgagtcttg acggcgctgt gtctcgcgcc gctggggacc ggtctcgcgc tcgcgctcgc
     1621 gtgcttgctg ccgtcgctct acgcggcggt gtggccaggg ctcgatcact gcacgcgcca
     1681 cgccgacgaa cacttgcatc tgtgtctggt gcatccgccg tcggctgtgg tcgggacagc
     1741 tgccgtcctc gtcggtgcga cctgtctgct gttcgtgctg ctgcgcgccg caccgggcgt
     1801 cgtcgatctg ttgcgggcgc gtcgggtgct cgcgcggctg cgggaggtaa gcgcggagac
     1861 cgtcctggcg ggggtgcgta gcgtcgacag cgacgccccg ttctcgttca ccggtggcct
     1921 gcttgcgccc gatatctatc tgtcccgcgg cttgcgcgag cgccttgagg cgccacagct
     1981 cgccgccgtg ctcgcgcacg agcgcgcgca tgttcagcgc cgcgatgtct tgcgtcgtca
     2041 tgtggcgcag ctcggggctc tgctgcacgc gccgggcacg gcgcgctcgc tggtgtcagc
     2101 gctcgatttg gcctgtgagg aggcatgcga cgccgaagct gcggctatca tcggcgatcg
     2161 cgcgcgggtc gccgaggcga tcgtcgcggt cgcgcgtctc gggccacgac caccgcccgc
     2221 actcgcggcg agcgtcgtcg gcttcaccgc ggacgccctg acgcaccgcg tgaacgctct
     2281 gctgtcaacg ccgcccccgg agtccaaggc gccatggtgg acgcttgcgg gaggcttttt
     2341 ggccctcgtg gtgctcgcac cggcgctgca ccatgcgacc gagacggtcc tgtcgttcct
     2401 ggcgcacccg tgaacctcat gccgcttcgt ctgcactcca tcaccgacaa aaaaccgagg
     2461 gcctatcggt gttcagcgct cctcggcttt gtcctcacgc tgctgatccg cacgggagcc
     2521 caggccgcgc cgacgactgc ggtcgacgag ccgaccgtgg tccgccgcgc gctcgctcgc
     2581 aatgcggtct cggacatcat cgagggcgac atcgaagcgg cgcgcgcgac cgcggtcgag
     2641 cttcgcacct ggcaaaaccc cgagctggct tacacccgcg agcagaccta cgggaccgcc
     2701 ggcaccggag aggactacct ctggctcgcg cagaccttcg acatcggcgg gcgccgcagc
     2761 ctccgcggcc gcgcggcgat gctgcgcagc gagggcgcgg cggcccgcgg cgagtcgctc
     2821 aaggtgctgc tcgcggccga ggtccgcctg cgctaccacg aggtcctcta tcggcgcctt
     2881 cgggtcgagg cgctcaccct ctggtcgcag cgtctgagca ccgcgctgac ggcgatcgcg
     2941 gcccgcgaga aggccggcga cgtggcgcgc tacgaccggc gccgactcga tcgcgagctg
     3001 gcgctcgccc gtgcgcggga ggaggtcgag gccaccgcgc tcgtcaaggc gtgggcccgg
     3061 ctcgccgcgc tggccgagct cgacgccgcc gccccgcagt ccgtgcccgg cgcgctgctg
     3121 ccggcgacgc gccccgaggc ggtcgaccag tacctcgccc gcgccgcgac gcgcccggac
     3181 atccgcgccc tcgagcgcgc cgaggcggcg accgagctcg actacaaggc ggcggcccgc
     3241 gcccgcgtcc cggagttgac gctcggcgct ggctggaagg gcgtgcaata caccggcctg
     3301 cgcagcgacg gcttcgtcgt caacgcctcc atgacgctgc cggtggtcga ccgtgggcag
     3361 ggtccgcggc gccgggcgga ggccgagggc ctggccctgc ggggtcgcgc cagcctggcc
     3421 cgccaggagc tcgccgggga gatcctcggg ctgcgcgccg aggccgaccg cttgcacgtc
     3481 ctggccgacc agttcacccg cgagcgccgc cgcgatgccg acgacctgat gcggatcgcc
     3541 gacgcggcgt accgcggcgg tgagctcggt attctcgagc tgctcgacgc cttccgcggc
     3601 gctgtcgacg atgagttgca gggcctcgag ctggccctga tggcccgccg cgcccgtatc
     3661 gagctcgagc gcgccgccgg aggcggcctc ccatgaaaca cgccctcgcc ctgctcctgt
     3721 gtctgctcat tcccgggtgc acccgcgacg tggccgaaga acacggagac catgcccacc
     3781 cccacgccga ggaggacccg cgccccggcc tcgtgttcac gaagtgggac gcgcacagcg
     3841 agctgttcgt cgagttcccg gcgctggtcg tcggtcaaga gtcgcgcttc gccgctcacc
     3901 tgacgcgcct gtccgacttc ggcgcggtcg acagcgggac gatgaccgcg gagctcggcg
     3961 acggcgcgga ggcgctgcgc gccgtcgcgg acgctccgag ccgccccggt attttccggc
     4021 cgacgctgac gccgacgcgc cccggcacgt ttcgtctcgt gctgcgctac gaaagtccgg
     4081 gcctggcagg cgcgcacgac ctcggcgaga tcacggtgta cccggacacc aacgcggccg
     4141 tcgcggcggc gcctccgggg gcggatctcg gcggcgttcc gttcctcaag gagcagcagt
     4201 ggcagatccc attcgccaca gagcctgcgg ccgcccggtc gcttcgtccg tcgatggccg
     4261 tctacgcgac cctgcgagcc cgccccgacg gcgaggtctc gatccacgcg ccggtggccg
     4321 gccgccttgg cgcgggcacc ggccgcttcc cgcagatggg cgcgcggatc accaaggacg
     4381 aggtgctcgc tgccatcgtg ccgcagctcg gccaggactc cgaccgcgcg accctcgacc
     4441 tcgccgtcca acaggcgcgt attgatgtcg acaacgcccg catcgagcgc gagcgcctcg
     4501 agagtctcct gaaagctggt gcgatccccg aacgccgcgt gatcgaggcc cgtcacagcg
     4561 agcgtgaggc cctcgcgctg cagaccgccg cttcgcgccg ccggggcacc ttcggccgcg
     4621 tacagcggac cggtggtggc gccggcgaca tcgccgtgcg cgccccgctg agcggcaccg
     4681 tcgtcgacgt gcagaccacg cccgggtcgt tcgtcgccgc gggccaggcc ctgctcgtcg
     4741 ccgccgatct cggccgcctg tggctggagg cccacgtgcc cgagcaccag gcgtccggcc
     4801 tcggcaaacc cgctggcgcg tggttccgcg tgccgggtat cgacgaggtc gtcgaggtcg
     4861 gcgccgaggg cctcgtcacc gtcgccggcc gcatcgaccc cgagacgcgc accctgccgg
     4921 tcgtgtttgc gatcgacaac ccgggtgagc gcctcccgat cggcaccttc gtggaggcgc
     4981 acctcgtcac cggtccgcca cgcgaggcgc tcgccgtgcc gaccgccgcg gtgctgaccg
     5041 aggcgggcca ggcagtcgtc tacgtgcagc ccgagggcga gcgcttcgag cggcgcccgg
     5101 tcgagctcgg cgtccaggac ggcgcctgga tcgaggtcgt tcgcggcgtc gcagccggcg
     5161 agcgggtcgt ctcgcgcggc gcttacctgg tccggctcgc aggcgccagc ggtaccgtcc
     5221 cgacccacgg gcacgcccat tgagccccgg agcccgatga tcgacgccgt catccgctgg
     5281 tcgctgcaaa atcgcctgct ggtgctgctc gcagccctcg cgctcgtcgt gtggggcagc
     5341 acgaccgtgc gccgcatgcc ggtcgacgtc ttccccgacc tcaccgcgcc gaccgtcacg
     5401 gtgctcgccg aggcccatgg catggccccg caagagctcg aggtccaggt cacctttccg
     5461 atcgaggccg cgctcaacgg cgccgccggg gtccgccgcg tgcgctcgtc gaccgcggtc
     5521 ggcatcgccg tgatctgggt cgagttcgag tggggccagg acccgtaccg tgcccgccag
     5581 atcgtcagcg agcgcctgca gctggtccgc gacgcgctgc cccccgaggt cgagccgccg
     5641 atcctggcgc cgatgtcctc gatcatgggc gagatcatgt tcgtcgccct gaccggcgag
     5701 cagcacgacc caatggatct gcgcacccag gccgactgga ccctgcgccg gcgcctgctc
     5761 gcggtgcccg gcgtttccca ggtgatcgtg ctcggcggcg acgtacgcca gtaccaggtc
     5821 cgcgtcgatc cggcgcgcct cgtcgcccac ggcctcggcc tcgaagacgt cacggcggcg
     5881 gtcggtgcga ccaacgccaa cagctccgcc ggcttcctga tcgagggcgg ccaggagtcg
     5941 atcatccacg gcatcggccg cgtgagccgc cccgaggaca tcgccgacac cgtgctggtc
     6001 gcccaccaga ccgcgcccgt acgggtcggc gacgtcgcca ccgtcgccct cggcccggcg
     6061 ctcaagcgtg gggacggctc gtacaacgct cgcccggcgg tcgtgatcgg cgtgcaaaag
     6121 cagccgggcg tcaacagcct cgagctgacc gcccgcatcg acgagctgct gaccgatctt
     6181 cagcccacgc tgccgtccgg catgcagatc ga
//
LOCUS       DASWNJ010000573         2587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_575613, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000573 DASWNJ010000000
VERSION     DASWNJ010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575613"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2005
                     /locus_tag="VGB85_09660"
     CDS             <1..2005
                     /locus_tag="VGB85_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEY0134336.1"
                     /translation="NSIIVETYIPGHDHRILVIGGRVIAVAKRVPGHVVGDGERTITQ
                     LVELVNSDPRRGIGHEKVLTRIELDHQARRLLELRGYTPESIPAPGEEVYLRSTGNLS
                     TGGTAIDLTDETHPDNREMAIRAANAIGLDIAGIDFITPDISRSYREVGGAICEVNAA
                     PGFRMHVAPTEGTPRDPAGPVMDMLFPPGTPSQIPIAAVTGTNGKTTVARMVAHIHKM
                     TGATVGLCTTDGVYIDAERTVVGDMTGPVAAQMVLRDPSVDLAVLETARGGLLRAGMG
                     FRSCNVGAVLNISPDHLGLGGIETLEQLAELKRIVVEVARDCAILNADDDYCLRMADY
                     TEAARIGYVTMNPRHDLVRRHIRAGGLAAVLEEGIAGDMITIYDRGTHLPLLWTHAIP
                     ATLDGKAIHNVSNAMFAALMTYAMGVKVENIRQGLRTFDTTFFQAPGRLNVYDELPFK
                     VILDYGHNPAAVRAMAALARRLDIKGKRTCVVAAPGDRRDEDIREIARAAAGAFNHFI
                     LREDDGLRGRAPGEVAKLIADELRQQGIERDRITGPYSEPESVEVGLSSCSPGDLLVI
                     FGDNVTRCWQQIVAFRERTAPGPARPKGPSQVGSLAPATRTPQSAVPANPLPRPPSAP
                     LLRADPEPEPEPVESHDPAALAAEGFVHDARGVRLARPQETSD"
     gene            2017..>2587
                     /locus_tag="VGB85_09665"
     CDS             2017..>2587
                     /locus_tag="VGB85_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134337.1"
                     /translation="MRRIDSRRLTGPNFHLGGPAAIAEVAFEPGEDPEAQIAAWRTAA
                     SAALTAIGWPAKFAVRRYTGADGQDHADLVFAGPEEALLLATDINDWAIDAASRSVES
                     GSPPDPADELEGWQEDLARVRDPALDALREAAAARGLPVLLDEERVSVGHGRHGLTWP
                     IDQIPDIESVPWDSLKRIPVALITGTNGKT"
BASE COUNT          397 a         1067 c          800 g          323 t
ORIGIN      
        1 caactcgatc atcgtcgaga cttacatccc cggccacgac caccgcatcc tcgtgatcgg
       61 cggccgcgtg atcgccgtcg ccaagcgcgt gcccggccac gtcgtcggcg acggcgagcg
      121 gacgatcacg cagctcgtcg agctcgtgaa cagcgacccg cgccgcggca tcggccacga
      181 gaaggtcctc acccgcatcg agctcgacca ccaggcgcgc cggctgctcg agctgcgcgg
      241 ctacaccccc gagagcatcc cggccccggg cgaggaggtg tacctgcgca gcaccggcaa
      301 cctgagcacc ggcggcaccg cgatcgacct caccgacgag acccaccccg acaaccgcga
      361 gatggcgatc cgcgccgcga acgcgatcgg cctcgacatc gccggcatcg acttcatcac
      421 ccccgacatc tcgcgctcgt atcgcgaggt cggcggcgcc atctgcgagg tcaacgccgc
      481 gcccggcttt cgcatgcacg tcgccccgac cgagggcacc ccgcgcgacc ccgccggccc
      541 ggtcatggac atgctgttcc cgcccggcac gccctcgcag atcccgatcg ccgcggtcac
      601 cggcaccaac ggcaagacca ctgtcgcccg catggtcgcg cacatccaca agatgaccgg
      661 cgcgaccgtc ggcctgtgca ccaccgatgg cgtctacatc gacgccgagc ggaccgtcgt
      721 cggcgacatg accggcccgg tcgccgcgca gatggtcctg cgcgacccct ccgtcgacct
      781 cgcggtgctc gagacggcgc gcggcggcct gctgcgcgcc ggcatgggct ttcgctcctg
      841 caacgtcggc gcggtgctca acatcagccc ggaccacctc ggcctcggcg gcatcgagac
      901 cctcgagcag ctcgcggagc tcaagcgcat cgtcgtcgag gtcgcccgcg actgcgcgat
      961 cctcaacgcc gacgatgatt attgcctgcg catggccgac tacaccgagg cggcccgcat
     1021 cggctacgtg acgatgaacc cgcggcacga cctggtgcgc cggcacatcc gcgccggcgg
     1081 cctcgcggcg gtgctcgagg agggcatcgc cggcgacatg atcacgatct acgaccgcgg
     1141 cacccacctg ccgctgctgt ggacccacgc gatcccggcg accctcgacg gcaaggcgat
     1201 ccacaacgtc tccaacgcga tgttcgccgc cctgatgacc tacgcgatgg gcgtcaaggt
     1261 cgagaacatc cgccagggcc tgcgcacctt cgacaccacc ttcttccagg cccccggccg
     1321 cctcaacgtc tacgacgagc tgcccttcaa ggtgatcctc gactacggcc acaaccccgc
     1381 cgccgtgcga gcgatggccg cgctggcgcg tcgcctcgac atcaagggca agcgcacctg
     1441 cgtggtcgcg gcccccggcg accgccgcga cgaggacatc cgggagatcg cccgcgccgc
     1501 cgccggtgcc ttcaaccact tcatcctgcg cgaggacgac ggcctgcgcg gccgggcccc
     1561 cggcgaggtc gccaagctga tcgccgacga gctgcgccag cagggcatcg agcgcgaccg
     1621 catcaccggc ccctacagcg agcccgagag cgtcgaggtc ggcctctcca gctgctcccc
     1681 cggcgacctg ctggtgatct tcggcgacaa cgtcacccgc tgctggcagc agatcgtcgc
     1741 cttccgcgag cggaccgccc ccggcccggc gcgccccaag ggaccctcac aggtcggcag
     1801 cctcgccccc gcgacgcgca cgccgcagtc tgcggtcccc gcgaacccgc tgccgcgccc
     1861 gccgtccgcg ccgctgctgc gcgccgaccc cgagcccgag cccgagcccg tcgaatctca
     1921 cgaccccgct gcgctcgccg ccgagggttt cgtgcacgat gcccgcggcg tgcgcctcgc
     1981 tcgcccgcaa gagacctccg actgacccga ctgaccatgc gccgcatcga ctcccgccga
     2041 ctcaccggtc cgaactttca cctcggtggc ccggccgcga tcgccgaggt cgccttcgag
     2101 cccggcgagg accccgaggc ccagatcgcc gcgtggcgca ccgccgcgtc cgcggccctc
     2161 accgcgatcg gctggcccgc caagttcgcc gtccgccgct acaccggggc cgacggccag
     2221 gaccacgccg acctcgtgtt cgccggcccc gaggaggccc tgctgctcgc caccgatatc
     2281 aatgactggg ccatcgacgc cgcctcgcgc agcgtcgaga gcggctctcc gcccgacccc
     2341 gccgacgagc tcgagggctg gcaagaggac ctcgcccgcg tgcgcgaccc ggccctcgac
     2401 gccctgcgtg aagccgccgc cgcccgcggt ctgccggtcc tgctcgacga ggagcgcgtc
     2461 tcggtcggcc acggccgcca cggcctgacc tggccgatcg accagatccc ggatatcgaa
     2521 tctgtcccct gggacagttt aaaaaggatc cccgtcgcgc tgatcaccgg caccaacggc
     2581 aagacca
//
LOCUS       DASWNJ010000574         2246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_10819, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000574 DASWNJ010000000
VERSION     DASWNJ010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10819"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VGB85_09670"
     CDS             <1..448
                     /locus_tag="VGB85_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY0134338.1"
                     /translation="GRRLDESDYAPMAALPTLASAIQIGAPVSLPKAVRALVECDGLV
                     EQANEQELAEAAAMADRTGMFNCPHTGVALACLRKLVAAGTIARNDRVVVVSTAHGLK
                     FTEFKARYHEDALGFTGSNVNRPHDVPVEPGQAVEAIHRLLDAVET"
     gene            complement(470..1453)
                     /locus_tag="VGB85_09675"
     CDS             complement(470..1453)
                     /locus_tag="VGB85_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134339.1"
                     /translation="MRTLILALIPGLLLACNNDITASAGTDDSTAGDSTGTSDPSSPS
                     DPSNPTDPTSPGTDPTTGASDPTNPTGQTTTTAGTATSEPGTTSPPPTTEPGTATSEA
                     TTAGTTDDTTAGDTDTDTGSGVCGGEGTVIDATLVHVGQDPGCGALEFTGSNYDLAPG
                     PIYALDGCPCGANCLKPDPWTFTLDVPQEWLPKAVPACPRIVVERQMSKAGCELVGVA
                     IWDMQEPEGAPAIYHAGSLLGPIAAAADQITIESVVAEECECADCCSVPTRWDLGFGG
                     LKSAVTIAEGQTGMLGDQDLGFEIINFQSHISGICDDSPAIDWVARRVGGL"
     gene            complement(1498..>2246)
                     /gene="tyrS"
                     /locus_tag="VGB85_09680"
     CDS             complement(1498..>2246)
                     /gene="tyrS"
                     /locus_tag="VGB85_09680"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEY0134340.1"
                     /translation="EFNYQLLQSYDFLHLYRNHGCTLQFGGSDQWGNMVAGIELIRRM
                     TPEDQAATPHCLTLPLLTTADGRKMGKTERGAVWLDAERLSPFDYYQFWVGCDDRDVA
                     KYLRVFTELPIAEIAELTQHEGAALNAAKARLAHEATRLLHGKAAADQAEAAARQAFG
                     GGDDWSAVPEVRAEVSELRLVELVTLPGVDAFKSRREAKQRIEQGAVKINGEPILDPA
                     RLLRSAEFEGGCLRLQAGKRTRFRVYLAGT"
BASE COUNT          280 a          738 c          877 g          351 t
ORIGIN      
        1 gggccgtcgt ctcgacgagt ccgattacgc gccgatggcg gccctgccga cgctggcctc
       61 ggcgatccag atcggcgcgc cggtgtcgtt gcccaaggcc gttcgcgcgc tcgtcgagtg
      121 cgacggcctc gtcgagcagg cgaacgagca ggagctggcc gaggcggcgg cgatggcgga
      181 ccgcacgggc atgttcaact gcccgcacac gggggtcgcg ctggcctgcc tgcgcaagct
      241 ggtcgccgcc ggcacgatcg cccgcaacga ccgcgtggtc gtggtctcga cggcgcacgg
      301 gctcaagttc accgagttca aggcgcgcta ccacgaggac gcgctcggct tcaccgggag
      361 caacgtcaac cggccgcacg acgtgccggt cgagcccggg caggcggtgg aggcgatcca
      421 ccgcctgctc gacgcggttg agacctgacc gtaaggacag gtccgacgat cacaggccgc
      481 cgacgcggcg ggcgacccag tcgatcgcgg gcgagtcgtc gcagatgccg ctgatgtgcg
      541 actggaagtt gatgatctcg aagccgaggt cctggtcgcc gagcatgccg gtctggccct
      601 cggcgatcgt gacggcggac ttgaggccgc cgaagccgag gtcccagcgg gtcggcacgc
      661 tgcagcagtc ggcgcactcg cactcctcgg cgacgacgct ctcgatcgtg atctggtcgg
      721 cggcggcggc gatcgggccg agcaggctgc cggcgtggta gatggcgggc gcgccctcgg
      781 gctcctgcat gtcccagatc gcgacgccga ccagctcgca gccggccttg ctcatctgcc
      841 gctcgacgac gatccgcggg caggcgggga ccgccttggg cagccactcc tgcggcacgt
      901 cgagggtgaa ggtccacggg tcgggcttga ggcagttggc gccgcagggg cagccgtcga
      961 gcgcgtagat cgggccgggg gcgaggtcgt agttgctgcc ggtgaactcg agcgcgccgc
     1021 agcccgggtc ctggccgacg tgcacgaggg tcgcgtcgat cacggtgccc tcgccgccgc
     1081 acacgccgct gccggtgtcg gtgtcggtgt cgcccgcggt ggtgtcgtcg gtcgtgccgg
     1141 cggtggtggc ctcgctcgtc gcggtgccgg gctcggtcgt cggcgggggc gacgtggtgc
     1201 ccggctccga ggtggccgtg ccggcggtgg tggtcgtctg gcccgtcggg ttggtcgggt
     1261 cggaggcgcc cgtggtcggg tcggtgccgg ggctggtcgg gtcggtcggg ttgctcgggt
     1321 cgctggggct gctcgggtcc gacgtgcccg tgctgtcgcc ggcggtgctg tcgtcggtgc
     1381 cggcgctggc ggtgatgtcg ttgttacagg cgagcagcag gccggggatc aaggcaagga
     1441 taagggttcg catctcgcca gagtaaccgc gcgcgcgccg gccgtcgagg tcggcggtca
     1501 ggtgccggcg agatacacgc gaaagcgcgt tcgcttgccg gcctgcaggc gaagacagcc
     1561 gccctcgaac tcggcgctgc gcaggagccg ggccggatcg aggatcggct cgccgttgat
     1621 cttcacggcg ccctgctcga tccgctgctt ggcctcgcgg cgcgacttga aggcgtcgac
     1681 gcccggcagc gtgacgagct cgacgagccg cagctcgctg acttccgcgc ggacctcggg
     1741 cacggcgctc cagtcgtcgc cgcccccgaa ggcctggcgc gcggcggcct cggcctggtc
     1801 ggcggcggcc ttgccgtgca gcaggcgcgt ggcctcgtgt gcgaggcggg ccttggcggc
     1861 gttgagggcc gcgccctcgt gctgcgtcag ctcggcgatc tcggcgatcg gcagctcggt
     1921 gaacacgcgc aggtacttgg cgacgtcgcg gtcatcgcag ccgacccaga actggtagta
     1981 gtcgaagggc gacaggcgct cggcgtcgag ccacacggcg ccgcgctccg tcttgcccat
     2041 tttgcgcccg tcggcggtgg tcagcagcgg cagcgtgaga cagtgcggcg tcgcggcctg
     2101 gtcctccggg gtcatgcggc ggatcagctc gatgccggcg accatgttgc cccattgatc
     2161 gctgccgccg aactgcaagg tgcagccgtg gttgcggtag aggtgcagga agtcgtacga
     2221 ctggagcagt tgataattga actcga
//
LOCUS       DASWNJ010000575         1890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_680739, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000575 DASWNJ010000000
VERSION     DASWNJ010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680739"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VGB85_09685"
     CDS             complement(<1..546)
                     /locus_tag="VGB85_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134341.1"
                     /translation="MTRPSRSTALLAMFFLASLACGPEKPPAPDRPASARSSARSPAG
                     SPAQPADAGSEAPILPAEPTDVIFSFAGERGAFKDANKVADVPEDARGLVRVTLLAGP
                     EPPPGRVWVANLRTPEPDGGWKLTTVERALFEELALGQGRASAIDKLAELPPGLEAPP
                     VAPAGADVIVYKTDWCGVCKQL"
     gene            579..>1890
                     /locus_tag="VGB85_09690"
     CDS             579..>1890
                     /locus_tag="VGB85_09690"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEY0134342.1"
                     /translation="MARFAPGGPPEGRSLLSLLRAVLALVLTLSVLTAILYTGYLTYT
                     DLSAPEVQAPELTPEVDRSGRRVTYGRSSLTRERALWLLTVEGSPEERGDAHGQLTSR
                     LFASLDAHVSDRLGQRYGGWLEAWGATMVMRWDFRDADDALPDAARRELAALAQAMPE
                     AAGGRLGSYHRLFLHQNFVELSRRLDDVLLEGSMFAASTRPKGGGERSNLVVGRNFSV
                     DLGAEVEPDRLVTFAHPDGKYPYVSIGWAGLLGVVTGVNARGVVVALNPSRTDDPLEN
                     GAPLPLVLRQVLEEADTLEQALEILQAADLRTSGAVLVGDGRQRKAAIVELAPRDKDE
                     RKPRGEDEAAIYATNHLVREPFERDAQNDRIRRYTSSGYRYERLGELLAEPGPVDPAR
                     AVAILRDRRGLGGEELGLGNRNALDNLAGRNLVIPLFNNLVISGF"
BASE COUNT          254 a          654 c          718 g          264 t
ORIGIN      
        1 cagctgcttg cagacgccgc accagtcggt cttgtagacg atgacgtcgg cgccggccgg
       61 cgcgacgggc ggggcctcga ggccgggcgg cagctcggcg agcttgtcga tcgcgctggc
      121 gcgaccctgt ccgagcgcga gctcttcaaa caatgcgcgc tcgacggtgg tcagcttcca
      181 gccgccgtcc ggctcggggg tgcggaggtt ggcgacccag acgcggccgg gcgggggctc
      241 ggggcccgca agcaaggtca cgcgcacgag gccgcgggcg tcctccggga cgtcggcgac
      301 cttgttggcg tccttgaagg cgccgcgctc gccggcgaag ctgaagatca cgtcggtcgg
      361 ctcggcgggc aggatcggcg cctcggatcc ggcgtccgcg ggttgtgcgg gggatcctgc
      421 gggggatcgg gcggaagatc gggcgctggc aggccggtcc ggggccggcg gcttctccgg
      481 gccgcacgcg agcgaggcga ggaaaaacat ggcgaggagg gcggtcgatc gggacggtcg
      541 tgtcatcgcg ggcgagggag tataacggct ggaccccgat ggcgcgattc gctcctggag
      601 gcccccccga gggccgtagc ctgctgagtc tgctccgcgc cgttttggcg ctggtgctga
      661 cgctgtcggt gctgacggcg atcctgtaca cgggctacct cacctacacg gatctctcgg
      721 cccccgaggt ccaggcgccg gagctcaccc cggaggtcga ccgcagcggc cggcgcgtca
      781 cgtacgggcg ctcgagcctg acgcgtgagc gggccctgtg gctgctgacg gtcgagggct
      841 cgccggagga gcggggcgac gcgcatggac aattgacctc gcggctgttc gccagcctcg
      901 acgcgcacgt gtcggaccgc ctcggccagc gctacggcgg ctggctcgag gcctggggcg
      961 cgacgatggt gatgcgctgg gacttccgcg acgccgacga cgcgctgccc gacgcggccc
     1021 gccgcgagct ggccgcgctc gcacaggcga tgcccgaggc cgcgggcggg cggctgggga
     1081 gctatcaccg actgttcctg caccagaatt tcgtcgagct gagccgccgc ctcgacgacg
     1141 tgctgctcga gggcagcatg ttcgcggcgt cgaccaggcc caagggcggc ggcgagcgca
     1201 gcaacctcgt cgtcggtcgc aacttctcgg tcgacctcgg cgcggaggtc gagcccgacc
     1261 gcctcgtcac cttcgcgcac ccggacggca agtaccccta cgtgtcgatc ggctgggccg
     1321 ggctgctcgg cgtggtcacc ggggtcaacg cgcgtggcgt ggtcgtcgcg ctcaacccct
     1381 cgcgcaccga cgacccgctc gagaacggcg ccccgctgcc gctggtgctc cgccaggtgc
     1441 tcgaggaggc cgacacgctc gagcaggcgc tcgagatcct gcaggccgcc gacctgcgca
     1501 cctcgggcgc ggtgctcgtc ggcgacggcc gccagcgcaa ggcggcgatc gtcgagctcg
     1561 cgccgcgcga caaggacgag cgcaagccgc gaggcgagga cgaggctgct atttacgcga
     1621 ccaatcacct cgtgcgcgag cccttcgagc gcgacgcgca gaacgaccgg atccgccgct
     1681 acacctcgtc ggggtaccgc tacgagcggc tcggggagct gctggccgag ccgggcccgg
     1741 tcgacccggc gcgcgccgtg gcgatcctgc gcgaccgtcg tggcctcggc ggcgaggagc
     1801 tcgggctcgg caaccgcaac gcgctcgaca acttggcagg ccgcaacttg gtcattcctc
     1861 tgttcaacaa cttggtgatc agcgggtttg
//
LOCUS       DASWNJ010000576         2005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_293512, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000576 DASWNJ010000000
VERSION     DASWNJ010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293512"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1464
                     /locus_tag="VGB85_09695"
     CDS             <1..1464
                     /locus_tag="VGB85_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0134343.1"
                     /translation="TGLGLSISREIARLLGGEIRVKSAQGAGSTFTFYVPQTHAPALT
                     SSATASSMNSTPAAMRPQIALAAAEAKFAEATPALLFPTEIADDREDIQPGDRVLLVI
                     EDDIPFAQLLLEMAREKGFKGLIALRGDTGLSLAREFKPDAITLDLRLPVLDGWTVLD
                     RLKHDARTRHIPVHLISGSDIDARGRALKQGALAFLQKPASREALAEALGTVKGFVER
                     RVRNLLIVEDNEIQRNALVELIGNGDVKTTAVGSAEAALDLLRTQHFDCMVLDLGLPD
                     MTGFDLINMIKSDEKLRELPIIVYTGKELSQEEETRLKRVTDAIIVKSVRSMEHLLDE
                     TALFLHRVEANLPQAKQQMLKQVHHADPVFSGKKVLIIDDDVRNIFAITSVLERYKMV
                     VVYAENGKRGIETLRENPDVNVVLMDVMMPEMDGYETTREIRKEQRFANLPIIALTAK
                     AMKGDREKCLEAGASDYITKPVEPEQLLSLLRVWLYG"
     gene            1457..>2005
                     /locus_tag="VGB85_09700"
     CDS             1457..>2005
                     /locus_tag="VGB85_09700"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEY0134344.1"
                     /translation="MAEKPPRRVTGELVGGVAVKDDPLLDPAVAEELEHGEVPGPALR
                     PASDLERVEIDLMLEGIYRHYGFDFRNYARASLRRRLWNQVRAEKLTTIASLLDKVLH
                     DVDCMERLLLMLSVNVTAMYRDPTFFRAFREQVVPLLRSHPFVRIWHAGCSTGEEVYS
                     MAILLEEEGLYDRCRLYATDMNE"
BASE COUNT          347 a          693 c          670 g          295 t
ORIGIN      
        1 accggcctcg gcctgtcgat ctcccgcgag atcgcccgcc tgctcggcgg cgagatccgg
       61 gtcaagtcgg cccagggcgc gggcagcacg ttcaccttct acgtgccgca gacccacgcg
      121 ccggcgctga ccagcagcgc gaccgcgtcg tcgatgaact cgacgcccgc ggcgatgcgc
      181 ccgcagatcg cgctggccgc cgccgaggcg aagttcgccg aggcgacccc ggcgctgctg
      241 ttcccgaccg agatcgccga cgaccgcgag gacatccagc ccggcgaccg cgtgctgctc
      301 gtcatcgagg acgacatccc cttcgcccag ctgctgctgg agatggcgcg cgagaagggc
      361 ttcaagggcc tgatcgcgct gcgcggcgac accggcctgt cactcgcccg cgagttcaag
      421 cccgacgcga tcaccctcga cctgcgcctg ccggtgctcg acggctggac cgtgctcgac
      481 cgcctcaagc acgacgcccg gacccgccac atccccgtgc acctgatctc gggcagcgac
      541 atcgacgccc gcggccgcgc gctcaagcag ggggcgctgg ccttcctgca aaagcccgcc
      601 tcgcgggagg cgctcgcgga ggcgctcggc acggtcaagg gcttcgtcga gcggcgggtg
      661 cgtaacctgc tgatcgtcga ggacaacgag atccagcgca acgcgctggt cgagctgatc
      721 ggcaacggcg acgtcaagac gaccgccgtc ggcagcgccg aggcggcgct cgacctgctg
      781 cgcacccagc acttcgactg catggtgctc gacctcggct tgccggacat gaccggcttc
      841 gacctgatca acatgatcaa gagcgacgag aagctgcgcg agctgccgat catcgtctac
      901 accggcaagg agctgtcgca ggaggaggag acccggctca agcgcgtcac cgacgcgatc
      961 atcgtcaaga gcgtgcgctc gatggagcac ctgctcgacg agaccgcgct gttcctgcac
     1021 cgcgtcgagg ccaatctccc ccaggccaag cagcagatgc tcaagcaggt ccaccacgcg
     1081 gacccggtgt ttagcggcaa gaaggtgttg atcatcgacg atgacgttcg caacatcttt
     1141 gccatcacca gcgtgctaga gcgctacaag atggtcgtcg tgtacgccga gaacgggaag
     1201 cggggcatcg agaccctgcg cgagaaccct gacgtgaacg tggtactcat ggacgtcatg
     1261 atgccggaga tggacggcta cgagaccacg cgggagatcc ggaaggagca gcgcttcgcc
     1321 aacctcccga tcatcgcgct gacggccaag gcgatgaagg gcgaccgcga gaagtgcctc
     1381 gaggccggcg cgtcggacta catcaccaag ccggtcgagc cggaacagct gctctcgttg
     1441 ctgcgcgtgt ggctgtatgg ctgagaagcc gccgcgccgc gtcacgggag agctggtcgg
     1501 gggcgtcgcc gtaaaggacg accccctgct cgacccggcc gtcgccgagg agctcgagca
     1561 cggcgaggtg ccgggcccgg cgctgcgtcc ggcctcggac ctcgagcggg tcgagatcga
     1621 cctgatgctc gagggcatct atcgtcacta cggcttcgac tttcgcaact acgcgcgggc
     1681 ctcgctgcgg cgccgcctgt ggaaccaggt gcgcgccgaa aagttgacga cgatcgcgtc
     1741 gctgctcgac aaggtcctgc acgacgtcga ctgcatggag cggctgctgt tgatgctctc
     1801 ggtcaacgtc acggcgatgt accgcgaccc gaccttcttc cgggcgttcc gcgagcaggt
     1861 ggtgccgctg ctgcgctcgc accccttcgt gcgcatctgg cacgccggct gctcgacggg
     1921 cgaggaggtc tactcgatgg cgatcctgct cgaggaggag ggcctctacg accgctgccg
     1981 gctgtacgcg accgacatga acgag
//
LOCUS       DASWNJ010000577         4178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_469533, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000577 DASWNJ010000000
VERSION     DASWNJ010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469533"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VGB85_09705"
     CDS             <1..187
                     /locus_tag="VGB85_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134345.1"
                     /translation="TPDEAGLARPTTIRALRERPSPDLPIRPEPSQLGLMLPMDALLA
                     AHPMADIIETLPLCPSA"
     gene            228..1076
                     /locus_tag="VGB85_09710"
     CDS             228..1076
                     /locus_tag="VGB85_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134346.1"
                     /translation="MRCLGLNQGYLQFGLSAFTMHDQWLPPPIALKYIPGLIEGPAFM
                     GWHPGRYLTHKVGKTTYFDGNCAVQQGHDVGYLIPHFAIPLNVMCLINTAFSKHKIMI
                     PVMSVLIEGKPMGTYLIAYLGLICASPVSLPTGVLIPLSCTVLSELTLKDIMIGLLFI
                     AVDIIIDAIWSLVVKGDKWGKFGLKGPHPAFYKAALDRIFNKAMTGPVAALITRQALN
                     KLADHLVKSWLVSPLVGGVIFQTGQPLLGVPRGSLPTVSVGRGSNLKHTFFPGSGSGH
                     AGNANN"
     gene            1092..2267
                     /locus_tag="VGB85_09715"
     CDS             1092..2267
                     /locus_tag="VGB85_09715"
                     /inference="COORDINATES: protein motif:HMM:NF021456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HEY0134347.1"
                     /translation="MSNKTETVMLAADAQQRQIVSVHAKRTYRLLASGRCVPAEVQQP
                     FLNMGGDEPEEGAPPELDIIPYKTTTDLIVMAEAHAPRGQTVTTMTASIELGKLKREY
                     LIHGDRRCIYRGPGSVRFDRPTPFESLPMRHELAFGGCDPTVPVPPPRTLLEAMSPHP
                     GIYPRNPSGRGYVVCDNRELIDGLLLPNIEHPVDRITPERLISGSVERWWRQPIPWSC
                     DWFDPTWYPRSTFLGGLPDFLPDDDSVVPEVMYGYVPRGQSQRIRDAEVHQLHDPRWA
                     DAAAPGLIVPFMRGDEAIRFRGMTPTGELVVRLPGERPRMTVTFEGETHELTPVPHRV
                     LISLEEMGVYIVWAGAWYPPRELPEHPVTAESAPGADLHGVVTKIDQRPVDPTGGAT"
     gene            complement(2312..2962)
                     /locus_tag="VGB85_09720"
     CDS             complement(2312..2962)
                     /locus_tag="VGB85_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134348.1"
                     /translation="MEGNHWQTLVIVLAVLVMAGGAVAMVLVGRSQRAGREQAARDVN
                     GVLRELATALHGELVPGKFLYQHPTLGELREYGVARVTSHKLAVEVTVNYPADDTHDH
                     RTVVRVPTPPHRAWRVTGLTMKRAPAADADLSSAATFERCIAGARAGDLPDPARVRLL
                     QLTRQALALELGAEGLRVTVAVDKPMFGGEWHETDQGRLRALVDEAALTAAELLSH"
     gene            complement(2976..3515)
                     /locus_tag="VGB85_09725"
     CDS             complement(2976..3515)
                     /locus_tag="VGB85_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134349.1"
                     /translation="MSGEDDRAGEGGEKASPTLAAVQRLALSSGYSVESNESLSEGVL
                     SIKAPDGRVFLKITLGADGPMVELQSASLSVAARGELTLDCQKLAINAAEGVAIRSGG
                     DIVQVAQGDVRVVAGGVIDSEGHAQNLRARRGDVQLTANDDVALDGERVRLNSPKPGV
                     HPREQMHAAAGPALPGPKP"
     gene            complement(3814..>4178)
                     /locus_tag="VGB85_09730"
     CDS             complement(3814..>4178)
                     /locus_tag="VGB85_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="RCC1 domain-containing protein"
                     /protein_id="HEY0134350.1"
                     /translation="QLGDGKGGTFESTFTPVDVSGLGSGVTAISAGTGFSTAVVSGGV
                     KYWGAYLDGTLTPLDVQGVTADMTAVATGADFACALVNDGAVKCWGNNNGGQLGIYVN
                     RDIGYHEEQTPVGVDSLP"
BASE COUNT          629 a         1583 c         1346 g          620 t
ORIGIN      
        1 gacgcccgac gaggccgggc tcgcccgacc gaccacgatc cgtgcgctgc gcgagcgtcc
       61 ctcccccgat ctgccgatcc gcccggagcc ctcgcaactc gggctcatgc taccgatgga
      121 cgcgctgctc gcggcccacc cgatggcgga tatcatcgag accctgccgc tgtgcccctc
      181 ggcctgaaac cccgaagccc gaaaaccccg aaagagaacc accgatcatg cgctgcctgg
      241 ggctcaatca aggatatcta cagttcgggc tgtcggcctt cacgatgcac gaccagtggt
      301 tgccgccgcc gatcgcgctc aaatacatcc ccggcctgat cgagggcccg gcgttcatgg
      361 gctggcaccc gggtcgatat ctgacccaca aggtcggcaa gaccacctac ttcgacggca
      421 actgcgccgt gcagcagggc cacgacgtcg gctacctgat cccgcacttc gcgatcccgc
      481 tcaacgtgat gtgcctgatc aacacggcgt tctcgaagca caagatcatg atccccgtca
      541 tgagcgtgct gatcgagggc aagccgatgg ggacctacct gatcgcctac ctcggcctga
      601 tctgcgccag tccggtgtcg ctgccgaccg gcgtgctgat cccgctgtcg tgcaccgtgc
      661 tctcggagct gaccctcaag gacatcatga tcggcctgct gttcatcgcc gtcgacatca
      721 tcatcgacgc gatctggtcg ctggtcgtca agggcgacaa gtggggcaag ttcggcctca
      781 aggggccaca cccggcgttt tacaaggcgg ccctcgacag gatcttcaac aaggcgatga
      841 ccggcccggt cgccgccctg atcacccgtc aggcgctcaa caagctcgcc gatcacctgg
      901 tcaagagctg gctcgtcagc ccgctggtcg gcggcgtcat cttccagacc gggcagccgc
      961 tgctcggcgt gccgcgcggc tcgctgccga cggtgtcggt cggccgcggc agcaacctca
     1021 agcacacctt cttccccggc agcggctcgg gccacgccgg caacgccaac aactgagcgc
     1081 aggtagccgg catgagcaac aagaccgaga ccgtgatgct cgccgcggac gcccagcagc
     1141 ggcagatcgt gtccgtgcac gccaaacgga cctaccgcct gctcgcgtcc ggccgctgcg
     1201 tgcccgccga ggtgcagcag ccgttcttga acatgggcgg cgacgagccc gaggagggcg
     1261 cgccgcccga gctcgacatc attccttaca agaccacgac cgacctgatc gtcatggccg
     1321 aggcccacgc gccgcgcggg cagacggtga cgacgatgac cgccagcatc gagctcggca
     1381 agctcaagcg cgagtacctg atccacggag accgtcgctg catctatcgc ggccccgggt
     1441 cggtgcgatt cgaccgcccg acgcccttcg agtcgctgcc gatgcgccac gagctcgcgt
     1501 tcggcggctg cgacccgacc gtgccggtgc cgccgccgag gacgctgctc gaggcgatgt
     1561 caccccaccc cggaatttat ccgcgcaacc cgtccggccg cggatatgtg gtgtgtgaca
     1621 atcgcgagct gatcgacggg ctgttgctgc cgaacatcga gcacccggtc gaccggatca
     1681 ccccggagcg cctgatctcc ggcagcgtcg agcgctggtg gcgacagccg atcccgtgga
     1741 gctgcgactg gttcgacccg acctggtacc cgcgctcgac cttcctcggc ggcctgcccg
     1801 acttcctccc cgacgacgac agcgtggtcc cggaggtgat gtacggatac gtgccgcggg
     1861 gccaaagtca gcgcattcgc gacgccgagg tccaccagct gcacgacccc cgctgggccg
     1921 acgccgcggc gccgggcctg atcgtcccgt tcatgcgcgg cgacgaggcg atccgctttc
     1981 gtggcatgac ccccaccggc gagctcgtcg tacgcctgcc cggcgagcgt ccgcgcatga
     2041 ccgtcacctt cgagggcgag acccacgagc tgaccccggt cccgcaccgc gtcctgatca
     2101 gcctcgagga gatgggcgta tatatcgtgt gggccggcgc ctggtacccg ccccgcgaat
     2161 tgcccgagca cccggtcacc gccgaatctg cgcccggcgc cgacctccac ggcgtcgtca
     2221 cgaagatcga tcagcgcccg gtcgatccca cgggcggcgc cacgtagcag cgcggggcac
     2281 gtagcagcgc gccgagcagc gccaccgggc ctcagtgaga cagcagctcg gccgccgtca
     2341 gcgcggcctc gtcgacgagc gcccgcaggc gcccctggtc cgtctcgtgc cactcgccgc
     2401 cgaacatcgg cttgtcgacc gcgacggtca cgcgcagccc ctcggccccg agctcgagcg
     2461 ccagcgcctg acgcgtcagc tgcaacagcc gcacccgcgc cgggtccggc aggtcgcccg
     2521 cgcgggcgcc ggcgatgcag cgctcgaagg tcgccgccga gctcaggtcg gcgtccgccg
     2581 ccggcgctcg cttcatcgtc aggcccgtca ctcgccaggc gcgatgcggc ggcgtcggca
     2641 cgcgcaccac cgtgcggtgg tcgtgggtgt cgtcggcggg atagttgacg gtgacctcga
     2701 ccgcaagctt gtggctcgtc acccgcgcga ccccgtactc gcgcagctcg ccgagggtcg
     2761 ggtgctgata caggaacttg ccgggcacga gctcaccgtg cagcgccgtc gccagctccc
     2821 tgagcacgcc gttgacgtcc ctggcggcct gctcgcgccc cgcccgctgc gagcgcccga
     2881 cgagcaccat cgccaccgcg ccgccggcca tcaccagcac cgccagcacg atgaccaggg
     2941 tctgccagtg gttgccttcc atcgcagcgc tccgactatg gtttgggccc ggggagagcg
     3001 gggccggcgg ccgcgtgcat ctgctcgcgg ggatgcacgc cgggcttcgg tgagttgagg
     3061 cgcactcgct ccccgtcgag cgcgacgtcg tcgttggcgg tcaactgcac gtcaccgcgg
     3121 cgcgcccgca ggttctgggc gtgcccctcg ctgtcgatca ccccgcccgc gaccacccgc
     3181 acgtcaccct gcgcgacctg gacgatgtca ccgccgctgc gaatggccac cccctcggcc
     3241 gcgttgatcg ccagcttctg gcagtcgagc gtcagctcgc cgcgcgccgc caccgacagc
     3301 gaggcgctct gcagctcgac catcggcccg tccgcgccga gcgtaatctt caggaagacc
     3361 ctgccgtcgg gggccttgat gctgagcacg ccctcggaca aagattcgtt ggactcgacc
     3421 gaatagccgg agctcagggc cagccgctgc acggcggcga gcgtcggcga agctttctcg
     3481 ccgccttcgc ccgccctgtc gtcctcgccg ctcaagcatc ctgcgatacc acgatttcgc
     3541 ggaccggcca agccgcgccg acgcgggccc gcgatcgcgc ccgcgtgccc ccgggtccgg
     3601 gcacggcgcc gcgaggcgcg gcggcggccc tcgctccgtc ggcgacggcg gtgtcccggt
     3661 gagagtgcgg cgcgcgtgcg cacccgcctg cccgggtcgg ccaacgtcgc tcggcgatat
     3721 gctcgtcgtg tccgcgatcc gaatgatcgc cctggacttc aaccgcttga tcgcgccgca
     3781 tcgcgccggc agggaaatcg cgtgcgccgc gattcagggc aggctgtcga ccccgaccgg
     3841 ggtctgctcc tcgtgatatc cgatgtcccg attgacgtag atgccgagct gacccccgtt
     3901 gttgttgccc cagcacttca ccgcgccgtc gttcaccagc gcgcacgcga agtccgcgcc
     3961 cgtcgccacg gcggtcatgt ccgcggtcac gccctgcacg tcgagcggcg tgagggtgcc
     4021 atccaggtac gcgccccagt atttcacgcc gcccgacacc acggcggtgg agaacccggt
     4081 gcccgcggag atcgcggtca cgccggatcc gaggcccgac acgtcgacgg gggtgaaggt
     4141 cgactcgaac gtacctccct tgccgtcgcc gagctggc
//
LOCUS       DASWNJ010000578         3079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_540879, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000578 DASWNJ010000000
VERSION     DASWNJ010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540879"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VGB85_09735"
     CDS             <1..922
                     /locus_tag="VGB85_09735"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0134351.1"
                     /translation="YLHHTPDGRPYADPNELSRQVIRAAESVGIRLVLLSVFYARAGA
                     GKAPLPEQRRFCDASLDAYLTRLDTLRDEGVQVGVAPHSVRAVPREALRELAAYAHRH
                     SLPLHMHVSEQPQENAECQAEHGCTPTALLAATGCLERAGGFTAVHAIHLEPADFVAL
                     AGHNVCACPTTEADLGDGIIPAGDHVRHGVGLALGSDSNAVIDLVQEARLLEMHERLR
                     TRARLCLAAADRPVGQTLLDIATTGGARALGRPELGQIRVGAPFDACTVDLAHPTLAH
                     LDPGDALDALLLSGTAAAIDRVFVGGVRRV"
     gene            999..1634
                     /locus_tag="VGB85_09740"
     CDS             999..1634
                     /locus_tag="VGB85_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134352.1"
                     /translation="MCSPRRLALVALIGLGGCAARDTAASRAAWQNAVRGASPGGAGA
                     GGSQGGRSDGGLADPGVWRDLATFTADTAELLALGVSTAPLATLVDKLCAEPPEEETG
                     MVEPEAVRCPPKPAMDPMGHPLTLELGRHGSVGLVATDLSDGDSAALLTQALKQMAGA
                     CAGEWERTAGPHHEEFHTCTSPSGSMLVLGRFPGEGAGERWQFSLAVLGPG"
     gene            1716..2909
                     /locus_tag="VGB85_09745"
     CDS             1716..2909
                     /locus_tag="VGB85_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134353.1"
                     /translation="MFRPTMLAAALAEVLIGKVRNIVDATVEISRHPSLPLALAQIYG
                     TVVGEDPAEFWRENLDLALVVSQIVPRQVFVYYARTGEDAREGFLVAQRGQAIAADDS
                     RREDIPPGKAGSHLPVTRLCEQMRISIDDLAGGFPGGPRIELSLMEPRGNDQTLLMTL
                     VGQQAAGAAEAELGEEEGYEDEPPPPPPPSRGPSLTQGFGAPPSGPAAPAAAPARQPP
                     GARPGAAQAKAPAMTAADDAKRRAADRAAEKQEIQQRSSELARSLKFAEDDRGIIVAL
                     PAELSETEVLRPFLVPHVERNAPEGLPADVRGRLEGKSIDFAVNVEFLSEVLIGKQPI
                     TKQKFEELATTRDLDGLEVKILEVLAPRLGAGTLLRSNGKNVFVSRKPDAPLPAKFLR
                     ELLAG"
BASE COUNT          413 a         1199 c         1084 g          383 t
ORIGIN      
        1 ctacctccac cacacgcccg acggccgccc ctacgcggac cccaacgagc tgtcgcgcca
       61 ggtgatccgc gcggccgagt cggtcggcat ccgcctggtg ttgctgtccg tgttctacgc
      121 gcgcgccggc gccggcaagg cgccgctgcc cgagcagcgc cgcttctgcg acgccagcct
      181 cgacgcctac ctgacccgcc tcgacaccct gcgcgacgag ggcgtgcagg tcggcgtcgc
      241 cccgcacagc gtgcgcgccg tcccgcgcga ggccctgcgc gagctcgccg cgtacgcgca
      301 ccggcattcg ttgccgctgc acatgcacgt gtcggagcag ccgcaggaga acgcagagtg
      361 tcaggcggag cacggctgca cgccgaccgc cttgctcgcg gcgaccggtt gcctcgagcg
      421 cgcgggcggc ttcacggcgg tgcacgcgat ccacctcgag ccggcggact tcgtcgcgct
      481 cgcgggtcac aacgtgtgcg cgtgcccgac caccgaggcc gacctcggcg acggcatcat
      541 cccggccggc gaccacgtgc gccacggcgt cggcctcgcg ctcggcagcg acagcaacgc
      601 ggtgatcgac ctggtccagg aggctcgcct gctcgagatg cacgagcgcc tgcggacgcg
      661 cgcgcgcctg tgcctcgccg ccgcggaccg gccagtggga cagaccctcc tcgacatcgc
      721 cacgaccggc ggggcgcggg ccctcggccg tccggagctg gggcagatcc gcgtcggcgc
      781 gcccttcgac gcgtgcaccg tcgacctcgc ccacccgacc ctcgcccacc tcgacccggg
      841 cgacgccctc gacgccctgc tgctcagcgg cacggcggcg gcgatcgacc gcgtgttcgt
      901 cggcggggtc cgccgcgtgt gaccgcgtgt gaccgggggg ggcgcgcgat cgcagcccgc
      961 tggccctgcg cgcccggcga tgctacgtcc cggggggcat gtgctcgccg cgtcgcctcg
     1021 ccctcgtggc cctgatcggg ctcggcggct gtgcggcgcg agacaccgcc gcctcgcgcg
     1081 ctgcgtggca aaacgcggtc cgtggcgcca gcccgggcgg ggccggtgcg ggtggctcgc
     1141 agggcggccg cagcgacggc ggcctcgccg accccggcgt ctggcgtgac ctcgccacct
     1201 tcaccgccga caccgccgag ctgctcgcgc tcggcgtctc gaccgccccg ctcgccaccc
     1261 tggtcgacaa gctgtgcgcc gagccgcccg aggaggagac cggcatggtc gagcccgagg
     1321 ccgtgcgctg cccgcccaag ccggcgatgg acccgatggg tcaccccctg acgctcgagc
     1381 tcggccgcca cggcagcgtc ggtctggtcg ccacggacct ctcggacggc gacagtgccg
     1441 cgttgctgac gcaggcgctc aagcagatgg cgggggcctg cgcgggcgag tgggagcgga
     1501 cagccgggcc gcaccacgag gaatttcaca catgcacctc gccatcgggg tccatgctgg
     1561 tgctggggag gtttcccggc gagggtgccg gggagcgctg gcagttctcg ctcgcagtat
     1621 tgggtccggg ctaatatgct cccgcgagct tcgcaatgga ccccacgacc tcgacagcgc
     1681 ccgtgagcca cggcgggatc ttgttcccgc tcgacatgtt ccgcccgaca atgctggcgg
     1741 ctgccctcgc cgaggtgttg atcggcaagg tccgcaacat cgtcgacgcc accgtcgaga
     1801 tctcgcgcca cccgagcctg cccctggcgc tcgcgcagat ctacggcacc gtcgtcggcg
     1861 aggacccggc cgagttctgg cgcgagaacc tcgacctcgc gctcgtcgtc agccagatcg
     1921 tcccgcgcca ggtgtttgtg tactacgcgc gcaccggcga ggacgcgcgc gagggcttcc
     1981 tcgtcgccca gcgcggtcag gcgatcgccg ccgacgacag ccgccgcgag gacatcccgc
     2041 ccggcaaggc cggctcccac ctgcccgtca cccgcctgtg cgagcagatg cggatctcga
     2101 tcgacgacct cgccggcggc ttccccggcg gcccgcgcat cgagctctcg ctgatggagc
     2161 cgcgcggcaa cgaccagacc ctcctgatga cgctcgtcgg acagcaagct gcgggcgccg
     2221 ccgaggccga gctcggcgag gaggagggct acgaggacga gcccccgcca cccccgccgc
     2281 cgtcgcgcgg cccgagcctg acgcagggct tcggcgcccc gccctcgggc ccggcagcac
     2341 ccgccgcggc gccggcacgc cagcctccgg gcgcgcgtcc gggcgcggcg caggccaagg
     2401 ccccggcgat gaccgccgcc gacgacgcca agcgccgcgc ggccgaccgt gccgccgaga
     2461 agcaggagat ccagcagcgc tcgagcgagc tggcgcgctc gctcaagttc gccgaggacg
     2521 accgcggcat catcgtcgcc ctgccggccg agctgtccga gacggaggtc ctgcggccgt
     2581 tcctggtccc acacgtcgag cgcaacgccc ccgagggcct gcccgccgac gtgcgcggcc
     2641 gcctcgaggg caagtcgatc gacttcgcgg tcaacgtcga gttcctcagc gaggtcctga
     2701 tcggcaagca gccgatcacc aagcagaagt tcgaggagct ggcgaccacc cgcgacctcg
     2761 acggcctcga ggtcaagatc ctcgaagtcc tcgccccgcg cctcggcgcc ggcacgctgc
     2821 tgcgcagcaa cggcaagaac gtgttcgtgt cgcgcaaacc ggacgcgccg ctgccggcca
     2881 agttcctgcg cgagctgctc gccggctaaa aggcccggag cgcctgccgt ctcgtctcac
     2941 aaagaggcgg caggcgcggt cagagctgga agcgcggtga ccctcgcccc tgccgccggt
     3001 ctcacgagga ggcgcgacgc aggggcggag ggatgcgcgg tggatggccc tcgccacggt
     3061 ctcgcaacga ggcgcaggg
//
LOCUS       DASWNJ010000579         1608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_116310, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000579 DASWNJ010000000
VERSION     DASWNJ010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116310"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..>1608)
                     /locus_tag="VGB85_09750"
     CDS             complement(37..>1608)
                     /locus_tag="VGB85_09750"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY0134354.1"
                     /translation="VCDLAITLAGAVSVPIYPSITAEQAAYILRDSGAVVAIVGDAGE
                     AERLRWVGPALQYVVIEGAGGEGSLDALQAAGEAVVAGQRETLDAVAADTTLSEPFTF
                     VYTSGTTGVPKGVVLTHKNLVYEAWAIKNVVAADRSDEQLMVLPLAHVFARHLLWGAV
                     EQGAVTAFAPPVERGASQTAALATAFQEVGPTYMCAVPRVYEKFYNQILREIQAQGAV
                     VGLGFEQAMEVGRKVSMCRQRGQALPGTLALKANLADRLFFTKIKQKFGGRLRFFVSG
                     GAPLRREVSEFFHAVGVLILEGYGLTETTGATNVNRPDRFRFGTVGPAMPGCELRIAD
                     DGEVLVRGAGVMQGYHGMPEETAAVLDEQGWLHTGDIGELRDGFLAITDRKKDLLVTS
                     NGKNIAPLPLEGRLELCEGIAHALVVGDARPYLAVLIALDVPAMLALARREGLNCRST
                     PEMARHPRIRQVIQGHIDAMNADLANFETIRRYAIADHEFSEATGELTPTQKLRRREV
                     LAKYAGVIEALYPVP"
BASE COUNT          230 a          582 c          557 g          239 t
ORIGIN      
        1 cgcagcatgc agcgcccgac cctatcatga tgcccgtcag gggaccgggt acagcgcctc
       61 gatgaccccg gcgtacttcg ccagcacctc gcggcgccgc agcttctgcg tcggggtcag
      121 ctcgccggtc gcctcgctga actcgtggtc ggcgatcgcg tagcgccgga tcgtctcgaa
      181 gttggccaga tcggcgttca tcgcgtcgat gtggccctgg atgacctggc ggatccgcgg
      241 gtgtcgggcc atctcggggg tgctgcggca gttgaggccc tcgcgccgcg ccagcgccag
      301 catggccggc acgtcgaggg cgatcagcac ggcgaggtac ggccgggcgt cgccgacgac
      361 cagcgcgtgg gcgatcccct cgcacagctc gaggcggccc tcgagcggca gcggggcgat
      421 gttcttgccg ttggaggtga cgagcaggtc ctttttgcgg tccgtgatcg ccaaaaagcc
      481 gtcgcgcagc tcgccgatgt cgccggtgtg cagccagccc tgctcgtcga gcaccgcggc
      541 cgtctcctcc ggcatgccgt ggtagccctg catgaccccg gcgccgcgga ccaggacctc
      601 gccgtcgtcg gcgatccgca gctcgcagcc gggcatcgcc ggaccgacgg tcccgaagcg
      661 gaaccggtcc gggcgattga cgttggtcgc gccggtcgtc tcggtcaggc cgtagccctc
      721 gaggatcagc acgccgaccg cgtgaaagaa ctcggacacc tcgcgccgca gcggcgcccc
      781 gccggacacg aagaagcgca gacggccgcc gaatttctgt ttgatcttcg tgaagaacag
      841 gcggtccgcg aggttcgcct tcaaggccag ggtgcctggc agggcctgcc cgcgctggcg
      901 gcacatgctg accttgcgac cgacctccat cgcctgctcg aagccgagtc cgacgaccgc
      961 cccctgggcc tggatctcgc gcaggatctg gttgtaaaac ttctcgtaaa cccgcgggac
     1021 ggcgcacata tacgtcggtc cgacctcctg aaaagcggtc gccagcgcgg cggtctgcga
     1081 ggccccccgc tccaccggcg gcgcgaaggc ggtgaccgcc ccctgctcga ccgcgcccca
     1141 cagcaggtgg cgggcgaaca cgtgggcgag cggcaggacc atcagctgct cgtcggagcg
     1201 gtcggccgcg accacgttct tgatcgccca ggcctcgtag acgaggttct tgtgggtcag
     1261 cacgaccccc ttgggcacgc cggtggtccc cgaggtgtag acgaaggtga agggctcgct
     1321 cagcgtcgtg tcggccgcga ccgcgtcgag ggtctcgcgc tgcccggcca ccaccgcctc
     1381 gcccgcggcc tgcaaagcgt cgaggctgcc ctcgccgccg gcgccctcga tcacgacgta
     1441 ttgcagggcc gggccgaccc aacgcaggcg ctcggcctcg ccggcgtcgc cgacgatcgc
     1501 gaccaccgcc ccgctgtcgc gcaggatgta ggccgcctgc tcggccgtga tcgacgggta
     1561 gatcgggacg ctgaccgctc cggccagcgt gatcgccagg tcgcagac
//
LOCUS       DASWNJ010000580         2032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_151911, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000580 DASWNJ010000000
VERSION     DASWNJ010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151911"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1963)
                     /locus_tag="VGB85_09755"
     CDS             complement(<1..1963)
                     /locus_tag="VGB85_09755"
                     /inference="COORDINATES: protein motif:HMM:NF037202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="HEY0134355.1"
                     /translation="MTPQALARSLAAVARGLRRALQRRLLAERRAGAGAVLEIQHEVA
                     RELVTLDDREFADAWAQTLVYLLLAARWLAPGQEPAEHLPQRLARCDARLGALVARLL
                     KVTEVQALQRALAGVTLVHAEVDDPVMHFYEAFLAAYDPRLRAARGVYFTPAPVVDYL
                     VETTDAVLRDRLGLSEGLADATSWGEYARRRGISVPPGVEETCPFVQVVDPAAGTGTF
                     VIAVIRRAHATMLARWGEDPAAWSEYVHTRLLPRLRGVERMIAPWAIAVLRLSMLLAE
                     TGVEGTLATPVFILGDTLAGPDLLPGPISVVLGNPPYERERPQAPGGGWIREGTGRAL
                     LADFVEPARAAGAGAHVKNLYNLYVYFWRWACWQVFERRPQAPGVVAMVTGASFLKGP
                     GFVGMRAHMRRWAPKIHVLDLEGDRRGANLSDNVFAVSVPVAATTLLRDLSDETGVEI
                     HRAQGSSADKLELCARTRRLDALAWMPVRGELAWIADEVSAGFHAWPKLTDLFPWQHS
                     GAQWKRTWPIAADPEVLARRWATLMRSEARGELFHETRDRSTDGQYPPLRGPGLGLAE
                     RTGEAISECEGVMPPVRRYGFRALDRRYCLADARLGDFLRPALWWIAGGRQVFLTSLL
                     TGALGPGPAAMVSVEVPDLHYFCGRGGKDV"
BASE COUNT          317 a          832 c          616 g          267 t
ORIGIN      
        1 tgacatcctt gcccccgcgc ccgcagaagt agtgcaggtc gggcacctcg acgctgacca
       61 tcgccgccgg tccgggcccc agcgccccgg tcagcaatga agtcaagaac acctgccgcc
      121 cgcccgcgat ccaccataac gccggacgta aaaaatcccc gagccgcgcg tccgcgaggc
      181 agtagcgccg gtcgagcgcc cggaacccgt agcgtcgcac cggcggcatc acgccctcgc
      241 actccgagat cgcctcacct gtcctttcag ccagccccag ccccggccca cgcaggggcg
      301 ggtactgccc gtcggtgctg cgatcgcggg tctcatgaaa cagctcgccg cgcgcctcgc
      361 tgcgcatcaa cgtcgcccag cgccgcgcca gcacctccgg gtccgcggcg atcggccagg
      421 tccgcttcca ctgcgccccc gagtgttgcc acgggaacag gtcggtcagc ttcggccagg
      481 catgaaagcc cgcggacacc tcgtctgcga tccacgccag ctccccgcgc accggcatcc
      541 aggccagcgc gtcgagcctc cgcgtccgcg cgcacagctc cagcttgtcc gccgagctcc
      601 cctgcgcgcg atggatctcg acacccgtct cgtctgacag atcgcgcaac aatgtcgtcg
      661 ccgcgaccgg caccgacacc gcgaacacgt tgtcactgag gttcgccccg cgtcggtcgc
      721 cctcgaggtc gagcacatgg attttcgggg cccatcgccg catatgcgcc cgcatgccga
      781 caaagcccgg cccctttaaa aagctcgccc cggtcaccat cgccaccacg ccgggtgcct
      841 gcggccggcg ctcgaacacc tgccagcagg cccagcgcca gaagtacaca tataaattat
      901 atagattttt gacgtgggcc ccggccccgg ccgcgcgcgc cggttcaacg aagtccgcga
      961 gcagcgcgcg tcccgtcccc tcgcggatcc acccgccgcc gggcgcttgc ggccgttcgc
     1021 gctcgtaagg aggattcccg aggaccacgc tgatcggccc cggcagcagg tcgggccccg
     1081 cgagcgtgtc gccgaggatg aacaccggcg tcgccagcgt cccctccacg ccggtctccg
     1141 ccagcagcat cgacagtcgc aacaccgcga tcgcccacgg ggcgatcatt cgttcgaccc
     1201 cacgcagccg cggcagcagc cgcgtatgaa catactccga ccaggccgcc ggatcctcgc
     1261 cccagcgcgc cagcatcgtc gcgtgcgcgc ggcggatcac cgcgattaca aacgtccccg
     1321 tgcccgcggc cgggtcgacc acctgaacga agggacaggt ctcctccacc cccggcggca
     1381 ccgagatccc gcggcgccgc gcgtactctc cccagctggt cgcgtccgcc agcccctcgc
     1441 tcaatcccag cctgtcgcgc aggacagcgt cggtcgtctc gaccaggtag tcgaccaccg
     1501 gcgccggcgt gaagtacacc ccacgggccg cgcgcagccg cgggtcgtag gccgccagga
     1561 acgcctcata aaagtgcatc accgggtcgt cgacctccgc gtgcaccagc gtcaccccgg
     1621 cgagcgcccg ctgcagcgcc tgcacctccg tcactttcag caacctcgcg accaacgcgc
     1681 ccaatctcgc gtcacaacgc gcgagccgct gcggcaggtg ctccgccggc tcctgtcccg
     1741 gcgccagcca gcgggctgcg agcagcaggt acaccagcgt ctgcgcccat gcgtcggcga
     1801 actcgcggtc gtcgagggtc accagctccc gggcgacctc gtgctggatc tcgagcacgg
     1861 ctccggcgcc cgcgcggcgc tccgccagca gccgccgttg cagcgcgcgt cgcagcccgc
     1921 gggccacggc cgcgagcgag cgggccagcg cttgcggggt catcggcccg cacgctagca
     1981 cccgcccgcg cgcctgtgca gctacactgg ccggcgatgt ctcgtcgcct cg
//
LOCUS       DASWNJ010000581         2454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_11046, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000581 DASWNJ010000000
VERSION     DASWNJ010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11046"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..>2454)
                     /locus_tag="VGB85_09760"
     CDS             complement(201..>2454)
                     /locus_tag="VGB85_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134356.1"
                     /translation="VAIAEALAAKLLRALPEHVASLRILAGSGVELYAPQLEPDGPDV
                     AFYLGAAASPPPRPRLVQPIAAPALTLAQLRPEPVAATLEASLGAAAELRMRVYTPAS
                     SEHHGAVEVVLHSSGRELGRFVLPSVHAGHVLVVELPAVAPSQLGARTAEEVHAAGEA
                     LATVCLRHASATLAEAADRLLAGLVDPSAPLGPVERQRALLALTRSAVLRLRRDRSGA
                     ATLCFALVEPGPPGVDLLGLPLFTAATGAALGARDLAARLVAGRGWLRVHDGSEFGVP
                     VDLEDLVLIDATTEPVLLRIIGESALLRVKSGWRGEGVAAVDADGRGYTIAAVEQALR
                     AGQRIVAHHAREIPRDWTPGEVVPDELWLPPWGWLRLARAGALLPALDFHLGDAEARE
                     HEDGQAFIASAPVTGAVEVVVCVPLVEPALPGVMVIDDEQRHVHRLAEIGHDFGVVGV
                     LRLRDPATEPEQVLAAIGAAVEKVYETLLVRVPAMDPQSPAFVRATATMLAHAGRRVS
                     LISDAHGRVRVPEVGELAGRILALPLFPGRRGLPLGAWQLIRCFAASGGELAAVEAML
                     DLAATPPVLRAWISETFARERVFHEPAASATVASPGLLELDKDGVVRPYPDPDLAAPL
                     DEITLATTAEYWLHHLRPDLAAKRPWDRRGRVYIDLEGGAGDELGEVTGEAKHWRLAL
                     HREHWLVRWAAKTGRDDREAIAWLLLACYARINEVFDDVTNNHEGMFQRAVADALVQG
                     RLAVVVPRIG"
BASE COUNT          330 a          982 c          880 g          262 t
ORIGIN      
        1 agcgcgcgac gccggcgtcg atctcgacgt cgaccgtgtg cgagacctcg accagcggct
       61 ggtcgcgcgt cgccgtcagg cggtcggcct gcgcggtccc ggccggggcc agggtggcga
      121 gcgccgagag gacggcgagg aggacgctgc tgcgcttcat cccgcgacag acaccggccg
      181 cggccgcagg ttccctgcgg tcaaccgatc cgcggcacca ccaccgcgag gcggccctgc
      241 accagcgcgt cggcgacggc gcgctggaac atgccctcgt ggttgttggt gacgtcgtcg
      301 aacacctcgt tgatgcgcgc gtagcaggcc agcagcagcc acgcgatcgc ctcacggtcg
      361 tcgcggccgg tcttcgcggc ccagcggacc agccagtgct cgcggtgcag cgccaggcgc
      421 cagtgcttgg cctcgccggt cacctcgccg agctcgtcgc ccgccccgcc ctcgaggtcg
      481 atgtacaccc gcccgcgccg gtcccacggg cgcttcgccg cgaggtccgg gcgcaggtga
      541 tgcagccagt actcggccgt cgtcgccagc gtgatctcgt cgagcggcgc ggcgaggtcg
      601 gggtccgggt acgggcgcac gacgccgtcc ttgtcgagct cgagcagccc gggcgacgcg
      661 accgtggcgg acgccgccgg ctcgtggaac acgcgctcgc gcgcgaacgt ctcggagatc
      721 cacgcgcgca gcaccggcgg cgtcgcggcg aggtcgagca tcgcctcgac cgccgcgagc
      781 tccccgcccg aggccgcgaa gcagcggatc agctgccacg cgccgagcgg caggccgcgg
      841 cggcccggga acagcggcag cgcgaggatg cggcccgcga gctcgccgac ctcgggcacg
      901 cgcacgcgac cgtgcgcgtc ggagatcagg ctgacgcgcc gcccggcgtg ggcgagcatc
      961 gtcgccgtcg cgcggacgaa ggccgggctc tgcgggtcca tcgccggcac gcggaccagc
     1021 agcgtctcgt ataccttctc caccgccgcg ccgatcgccg cgagcacctg ctccggctca
     1081 gtcgcggggt cgcgcaggcg cagcaccccg accacgccga agtcgtggcc gatctcggcg
     1141 aggcgatgca cgtgccgctg ctcgtcgtcg atcaccatca caccgggcag cgccggctcg
     1201 accagcggca cacagaccac gacctcgacc gcgccggtga ccggcgccga ggcgatgaac
     1261 gcctgcccgt cctcgtgctc gcgcgcctcc gcgtcgccga ggtggaagtc gagcgcgggc
     1321 aacagcgccc ccgcccgcgc cagccgcaac cagccccacg gcggcagcca cagctcgtcc
     1381 ggcacgacct cgcccggggt ccagtcgcgc gggatctcgc gcgcgtggtg agcgacgatc
     1441 cgctggccgg cgcgcagcgc ctgctcgacc gccgcgatcg tgtagccgcg accgtccgcg
     1501 tcgacggccg cgacgccctc tccccgccac ccgctcttga cgcgcagcag cgccgactcg
     1561 ccgatgatcc gcaagagcac tggctcggtc gtcgcgtcga tcaacacgag gtcctcgagg
     1621 tcgaccggga cgccgaactc gctgccgtcg tgcacacgca accaaccccg gcctgccacc
     1681 aaccgcgccg cgaggtcacg cgcgccgagg gccgcgccgg tcgccgcggt gaacagcggc
     1741 aggccgagca ggtcgacccc cggcggcccc ggctcgacga gcgcgaagca cagcgtcgcc
     1801 gccccgctgc gatccctgcg caggcgcagc accgccgagc gcgtcagcgc cagcagggcc
     1861 cgctggcgct cgaccggccc gagcggcgcc gagggatcga cgagacccgc gagcaggcgg
     1921 tcggccgcct cggccagcgt cgcgctcgcg tgccgcagac acaccgtcgc caaagcctcg
     1981 cccgccgcgt gcacctcctc ggccgtgcgc gccccgagct gcgacggcgc caccgccggc
     2041 agctcgacca ccagcacgtg ccccgcgtgc accgaaggca gcacgaagcg gccgagctcg
     2101 cggccgctgc tgtgcagcac gacctcgacg gccccgtggt gctcgctgct cgccggcgta
     2161 tacacgcgca tgcgcagctc cgcggccgcg ccgagcgacg cctcgagcgt cgcggcgacc
     2221 ggctcgggcc gcagctgcgc gagcgtcagc gccggcgcgg cgatcggctg gaccagccgc
     2281 ggtcgcggtg gcgggctcgc agcggcgccg agatagaacg cgacatcggg cccgtcgggc
     2341 tcgagctgcg gcgcgtacaa ttcgaccccg ctgccggcga ggatccgcag cgaggcgacg
     2401 tgctccggca gggcgcgcag cagcttggcc gccagcgcct ccgcgatcgc cacc
//
LOCUS       DASWNJ010000582         4314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_258842, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000582 DASWNJ010000000
VERSION     DASWNJ010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258842"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..1786
                     /locus_tag="VGB85_09765"
     CDS             263..1786
                     /locus_tag="VGB85_09765"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEY0134357.1"
                     /translation="MALELHFDHGTLVAPGVLPPDERLAQLLVPDSRTGSHRAPAHRY
                     REIVGRLHRSGVPYQDLAKNYERIELPLAAPLAPFPHQQASLDAWVAGGCTGIVELPT
                     GAGKTLLAVLAIQHVGRPALIVVPTIDLMLQWQQVLQKWFDRPIGMLGGGVHDRRELT
                     VTTYDSAAAQTEFHGNRFGLLICDECHHLPAPAYRFIAAGSLAPYRLGLTATLDRTDG
                     GEAIAMDLLGPVRFRASIDELEGSYLAPYEIHSIEVALSEEEQLRYDECRAIYVDALR
                     SSGVSLNHPSGWAQFIAHCYRTEEGRLAFQAYRDQRRIAFTAQSKVETLWRVLARHRS
                     DRILVFTEDNDTVYRLSRLLMLPVITHQTKPAERVELLRGFASGDLPVLLTSKVLNEG
                     VDVPDANVGVILSGNGSVREHVQRLGRILRKREGKRATLYEICTAVAAEGRISERRRQ
                     HRAYQRGEPVPHHHLPEVDPDFETGVEPGDYSGNFASESDPSASWGPPDDAPESGAC"
     gene            1780..3006
                     /locus_tag="VGB85_09770"
     CDS             1780..3006
                     /locus_tag="VGB85_09770"
                     /inference="COORDINATES: protein motif:HMM:NF017442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF790 family protein"
                     /protein_id="HEY0134358.1"
                     /translation="MLTRELLIYRRVKGKLRPTFVDIKHEDHLALAAGLVQVLEHGVG
                     ETRGLLEEALGVLVGESKKPKVARGLVKLLLDKCSFEEPGDAAAALRKQAFQASATAL
                     RGLPDDAPLALYLERLSAGLPQPLELCRAGLYDDLGEHRRMIRCALPTPPALLERYNL
                     AQAQGLVLYAQRLCVRTGTASQLELRKLLRWLKFSRLVAEVRREGDDWLLTVEGPGAM
                     FDMQKKYGLQLATFLAAVPLLSQFTLEAEVDIPRGAKGTLVLDHKDPLRALDRSALGH
                     IPPEIEVGLASLADERWSIDPLPELRHTGALGMCVPDFGLRDAVSGAAVAVELFHRWH
                     QRALLRRLDELAARPDPALLLGVDLSLVKDADLAARVRDHLQVFTFNKFPARKRIQPL
                     LDRAAAAGTARSAART"
     gene            3143..>4314
                     /locus_tag="VGB85_09775"
     CDS             3143..>4314
                     /locus_tag="VGB85_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134359.1"
                     /translation="MHRRPSQLPFAAPIATPISVALLGVSLGILLAPTLAHAQAPMPM
                     GPTAPPADAFDIRYYTTDAEGNRSRPLDQVDFTRFINKARCECGHQISTRIVLKPAMG
                     TTYNLSTQLYTFVGTMCGAAESAPIGGQFRLCAQLKSGNPPQFQAEYNTSFHPIWLSS
                     GVALASSADRNPSTAIAANSCENAEGQSGIWMCANTNGVSGCQSDEFFITGSQNNNVP
                     AGMASGIAFDYLPPQTVPTDLTALPGDSAVIVSWGVLSGDIAGFRVLCEEADTGKPPP
                     GKGYIGKDITAKQDGTIYYTKDNLCPGAPFSTFKAGNENPLDPNAPTDDTGATTDDSA
                     GTTTDASGTTTGDSGTTDDTAGSSGSSGGVSLTCGDGVLDAGEDCDDGENDGGYGE"
BASE COUNT          654 a         1732 c         1303 g          625 t
ORIGIN      
        1 gtcgaccccg tcgactcgct cgtgctggtg cccgggccgc agcccgccga catcacgctc
       61 gccatcacca gcgcaccgcg cagccatcgc ccggaagtca cgtccatcgt ctcggtcctc
      121 cgatgtgcac cctatcaaga acccggctgg cgcccacgcg ccatcttcgc cgtcggtttc
      181 atggcgcgcg cgcgcggccc cgtgcgcatt cgcgccgtga tggtgctagt ctccgccgcc
      241 cgacccccgt cgttccgcgc ccatggccct cgagctccac ttcgatcacg gcaccctcgt
      301 ggcccccggc gtcctgccgc cggacgagcg tctggcccag ctgctcgtgc ccgacagccg
      361 caccggcagc catcgcgcgc cggcccaccg ctaccgcgag atcgtcggtc gcctgcaccg
      421 cagcggcgtc ccgtatcagg acctcgccaa gaattacgag cgcatcgagc tcccgctcgc
      481 cgccccgctc gccccgttcc cacaccagca agcctcgctc gacgcgtggg tcgccggcgg
      541 ctgcacgggc atcgtcgagc tgccgaccgg cgccggcaag accctgctcg cggtgctggc
      601 gatccagcac gtcggtcgcc ccgcgctgat cgtcgtgccg acgatcgacc tgatgctgca
      661 atggcagcag gtcctgcaaa aatggttcga ccgcccgatc ggcatgctcg gcggcggcgt
      721 gcacgaccgc cgcgagctga ccgtcacgac ctacgactct gcggccgcgc agaccgagtt
      781 ccacggcaac cgcttcggcc tgctgatctg cgacgagtgc catcatttgc ccgcgccggc
      841 gtatcgcttc atcgccgccg gctcgctcgc gccgtaccgc ctcggcctga ccgccaccct
      901 cgatcgcacc gacggcggcg aggcgatcgc catggacctg ctcggcccgg tccgcttccg
      961 cgccagcatc gacgagctcg agggcagtta cctcgcccct tatgaaattc actcgatcga
     1021 ggtcgcgctg tccgaggagg agcagctgcg ctacgacgag tgccgcgcga tctacgtcga
     1081 tgccctgcgc agcagcggcg tctcgctcaa ccatcccagc ggctgggcgc agttcatcgc
     1141 ccactgttat cgcaccgagg agggccgcct cgccttccag gcctaccgcg accagcgacg
     1201 gatcgccttc accgcgcagt ccaaggtcga gaccctgtgg cgcgtgctcg cccgccaccg
     1261 cagcgaccgc atcctcgtgt tcaccgagga caacgacacc gtctatcgcc tctcgcgcct
     1321 gctgatgctc ccggtgatca cccaccagac caagccggcc gagcgcgtcg agctcctgcg
     1381 cggctttgcc tcgggcgacc tgccggtcct gctgaccagc aaggtgctca acgagggcgt
     1441 cgacgtcccg gacgccaacg tcggcgtgat cctgtccggc aacggcagcg tccgcgagca
     1501 cgtgcagcgc ctcggacgca tcctgcgcaa gcgcgagggc aagcgcgcca ccctctacga
     1561 gatctgcacc gccgtcgccg ccgagggccg catcagcgag cgccgccgac agcaccgcgc
     1621 ctaccagcgt ggcgagcccg tgcctcacca ccacctcccc gaggtcgacc ccgacttcga
     1681 gaccggcgtc gagcccggtg actactctgg caatttcgcc agcgagtccg acccgtccgc
     1741 gagctggggt ccgcccgacg acgccccgga gtccggcgca tgctgacccg cgagctgctg
     1801 atctatcgcc gcgtcaaggg caagctgcgc cccaccttcg tcgacatcaa gcacgaggac
     1861 cacctcgccc tcgccgccgg cctcgtgcag gtgctcgagc acggcgtcgg cgagacccgc
     1921 ggcctccttg aagaggccct cggcgtgctc gtcggcgagt ccaaaaagcc caaggtcgcg
     1981 cgcggcctcg tcaagctgtt gctcgacaag tgcagcttcg aggagcccgg cgacgccgcg
     2041 gccgcgctgc gcaaacaggc cttccaggcc agcgccacgg ccctgcgcgg cctcccggac
     2101 gacgcaccgc tcgcgctcta cctcgagcgc ctctcggccg gcctcccgca gccgctcgag
     2161 ctgtgccgcg ccggcctcta cgacgacctc ggtgagcacc ggaggatgat ccgctgcgcc
     2221 ctgccgaccc cgccggccct gctcgagcgc tacaacctcg cccaggccca gggcctcgtg
     2281 ctctacgccc agcgcctgtg cgtgcgcacc ggcaccgcca gccagctcga gctgcgcaag
     2341 ctcctgcgct ggctcaagtt cagccgcctc gtcgccgagg tccgccgcga gggcgacgac
     2401 tggctgctga ccgtcgaggg ccccggcgcc atgttcgaca tgcagaagaa gtacggcctg
     2461 cagctcgcga ccttcctcgc cgccgtgccc ttgctgagcc agttcacgct cgaggccgag
     2521 gtcgacatcc cacgcggcgc caagggcacc ctcgtgctcg accacaaaga tcccctgcgc
     2581 gcgctcgacc gcagcgcgct cggccacatt ccgccagaga tcgaggtcgg cctcgcctcg
     2641 ctcgcggatg agcgctggag catcgacccg ctgccggagc tgcgccacac cggcgcgctc
     2701 ggcatgtgtg tccccgactt cggcctgcgc gacgccgtct ccggggccgc cgtcgccgtc
     2761 gagctgttcc atcgctggca ccagcgcgcc ctgctgcgcc gcctcgacga gctcgccgcg
     2821 cgccccgacc ccgccctgct cctcggcgtc gacctcagcc tcgtcaagga cgccgacctc
     2881 gccgcgcggg tccgcgacca cctccaggtc ttcaccttca acaagttccc cgcccgcaag
     2941 cggatccagc cgctgctcga ccgcgccgca gccgccggaa cggcacgcag cgccgcccgg
     3001 acctgacatt ttgtcatccg gccacacgtg acccggcgct cgcatggccc gtagctggcc
     3061 gccggccggt gatgcgggtc tgtggcatcc tggttgcacg ggggtgttcc gcttgccgag
     3121 ccaccctccc ccaccggtct ttatgcatcg acggccctca caacttccgt tcgccgcccc
     3181 gatcgccacc ccgatctcag tcgcgctgct cggcgtctcg ctgggcattt tgctggcgcc
     3241 gactctggcc cacgcacagg ccccgatgcc gatgggcccg acggcgccgc cggccgacgc
     3301 cttcgacatt cgctactaca ccaccgacgc cgagggcaac cgcagccggc cgctcgacca
     3361 ggtcgacttc acgcgcttca tcaacaaggc ccgctgcgag tgcggccacc agatctcgac
     3421 ccgcatcgtg ctcaagccgg cgatggggac cacgtacaac ctctcgacgc agctttacac
     3481 cttcgtcggg acgatgtgcg gtgccgcaga gtcggccccg atcggtggtc agttccgtct
     3541 ctgcgcccag ctcaagtccg gcaaccctcc gcagttccag gccgaataca acaccagctt
     3601 ccacccgatc tggctcagca gcggcgtggc ccttgcgtcc tcggcagacc gtaatccaag
     3661 caccgccatc gccgctaaca gctgcgagaa cgccgagggc cagtccggca tatggatgtg
     3721 cgccaacacc aacggtgtct cgggctgcca gagcgacgaa ttcttcatca ccggctcgca
     3781 gaacaacaac gtcccggccg gcatggcctc gggcatcgcc ttcgactatt tgccgccgca
     3841 gaccgtcccg acggacctca cggccctgcc cggtgactcc gccgtgatcg tcagctgggg
     3901 agtcctgtcg ggcgacatcg ccggctttcg cgtgctgtgc gaggaggccg acaccggcaa
     3961 gccgcccccc ggcaagggct acatcggcaa ggacatcacc gccaagcagg acggcacgat
     4021 ctactacacc aaggataacc tgtgccccgg cgccccgttc tcgaccttca aggccggcaa
     4081 cgagaatccg ctcgacccga acgcgcccac cgatgacacc ggcgcgacca ctgatgacag
     4141 cgctggcacg accacggacg ccagcggcac gaccacgggt gatagcggca ccaccgatga
     4201 caccgccggc agcagcggct ccagcggcgg cgtctcgctg acctgcggcg acggcgtcct
     4261 cgatgccggc gaggactgcg acgacggcga gaacgacggc ggctacggcg agtg
//
LOCUS       DASWNJ010000583         1836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_435516, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000583 DASWNJ010000000
VERSION     DASWNJ010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435516"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /locus_tag="VGB85_09780"
     CDS             <1..1242
                     /locus_tag="VGB85_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY0134360.1"
                     /translation="SLPDGLPEEPLVTQLHARRLTPYYQDVEDCRLPRIHRELLQQAD
                     YTSRRAEIDAFNARSPHVKRGLGFMPVKFGISFTNSILNQAGALVLVYTDGSVQLNHG
                     GTEMGQGLHSKMLAVCAHELGVRQSQIRVMPTATDKVPNTSATAASSGSDLNGAAVQR
                     ACVELRERLRPLAAELLGLTDPQQVSFADGQVFAPGGRSTSFAELTRAAWARRISLSA
                     AGYYATPGITYDRELGRGKPFHYFAYGACLTEVEVSGLTGEHRVLRVDILHDAGRSLL
                     PSIDRGQVEGGYIQGLGWLTCEELVSDSRGRLLTHGPSTYKIPAVGDAPEDFRVTLLT
                     DAAQDNVIHGSKAVGEPPFMLAISVVAALRHAVTGFGAAGQGPRLAIPCTPEAILRAI
                     EDMRARAGMARTDAEMISAAE"
     gene            1278..>1836
                     /locus_tag="VGB85_09785"
     CDS             1278..>1836
                     /locus_tag="VGB85_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134361.1"
                     /translation="MTADSFVFNPYGPAFDADPAPVYRHLHEHVPVYWWERGRAFIVS
                     RHADVVALMKDPRFSRSPADGRDYQPIPDVPELHDFRVASDNSLFFAAPADHLRQRRL
                     MNSAFSPRAIQWLREHTAELTRQALDSLPAAEVVNLAPVADYIPLRVIGRLLDIPAER
                     EDSFLAFAQARIALLSPGLPPAYREQ"
BASE COUNT          272 a          680 c          609 g          275 t
ORIGIN      
        1 agcttgcccg acggactgcc cgaggagccg ctggtcaccc agctgcacgc gcgccgcctg
       61 acgccttatt atcaagacgt cgaggactgc cgcctgccgc ggatccatag ggagttgctg
      121 cagcaggccg actacacgag ccggcgcgcc gagatcgacg cgttcaacgc ccgctcgccg
      181 cacgtcaagc gcgggctcgg cttcatgccg gtcaagttcg gcatctcctt tacaaattcg
      241 atcctcaacc aggccggcgc gctcgtgctc gtgtacacgg acggcagcgt gcagctcaac
      301 cacggcggca ccgagatggg ccagggcctg cacagcaaga tgctggcggt ctgcgcccac
      361 gagctcggcg tgcgacagtc gcagatccgc gtgatgccga ccgcgaccga caaggtcccg
      421 aacacctcgg cgaccgcggc ctcgagcggc tcggacctca acggcgccgc ggtgcagcgc
      481 gcctgcgtcg agctgcgcga gcgcctgcgt ccgctcgcgg ccgagctgct cggcctgacg
      541 gacccgcagc aggtcagctt cgccgacggc caggtgttcg cgcccggcgg ccgcagcacc
      601 agcttcgccg agctgacccg cgccgcctgg gcccgaagga tctcgctgtc ggccgcgggc
      661 tactacgcga ccccgggcat cacgtacgac cgcgagctcg ggcgcggcaa gcccttccac
      721 tacttcgcgt acggcgcgtg cttgaccgag gtcgaggtct cgggtctcac cggcgagcac
      781 cgggtgctgc gcgtcgacat cctgcacgac gccggccgct cgctgctgcc gagcatcgac
      841 cgcgggcagg tcgagggtgg ctacattcag ggcctgggct ggctgacctg cgaggagctg
      901 gtctcggaca gccgcggtcg cctgctgacc cacggcccct cgacatacaa gatccccgcc
      961 gtcggcgacg cgccggagga ctttcgcgtc acgctgttga ccgatgcggc gcaggacaac
     1021 gtcatccacg gcagcaaggc cgtcggcgag ccgcccttca tgctggcgat ctcggtggtc
     1081 gcggcgctgc gccacgcggt caccggcttc ggtgccgcgg gccaggggcc acgcctggcg
     1141 atcccgtgca ccccggaggc gatcctgcgc gcgatcgagg acatgcgcgc acgcgcagga
     1201 atggcgcgca cggacgcgga gatgatcagc gccgccgagt agccaccccg cgagggtttt
     1261 gcgaagctcg tggtccgatg accgccgaca gcttcgtgtt caacccctac ggcccggcgt
     1321 tcgacgccga tccggcgccc gtctatcgtc acttgcacga gcatgtgccg gtgtactggt
     1381 gggagcgcgg tcgggctttc atcgtcagca gacacgccga tgtcgttgca ttgatgaagg
     1441 acccgcgctt ctcgcggtcg ccggccgatg gtcgtgacta ccagccgatc cccgacgtgc
     1501 ccgaactcca cgacttccgc gtcgcctccg acaacagcct gttcttcgcg gcgccggctg
     1561 accacctgcg ccagcgccgg ctgatgaact ccgccttctc gccgcgcgcg atccagtggc
     1621 tgcgcgagca caccgccgag ctgacgcggc aggcgctcga cagcttgccc gcggccgagg
     1681 tcgtcaacct cgcgccggtc gctgactaca tcccgctacg cgtcatcgga cgcctgctcg
     1741 acatccccgc ggagcgcgag gacagcttcc tggcgttcgc gcaggcccgc atcgcgctgc
     1801 tcagtccggg actgccgccg gcctatcgtg agcaga
//
LOCUS       DASWNJ010000584         4571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_187653, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000584 DASWNJ010000000
VERSION     DASWNJ010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187653"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="VGB85_09790"
     CDS             <1..1085
                     /locus_tag="VGB85_09790"
                     /inference="COORDINATES: protein motif:HMM:NF020071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0134362.1"
                     /translation="PARRVLQVRDQLLRLCEGHARKDRSQNNASLQAREDSLRHALLV
                     AFPDRVAQARDDATGGRALALAGGGAALLSPASVVRTAPWLLALAVEQRREGERGSQA
                     VVRSACAIDAEWLVDHFPDEIDAIDLLTFNAARERVEGRSELRWHGLLIEASPHKKLP
                     PAAATVLRDAALAAGPARFVGDPDALAGLMARTTFVASHVPEFPTLDDAAVRDALAEL
                     CHGRSSFAELRAADLYCTLLARVAGLHGNALERMAPTHILLPGGRRLAVHYEPGKPPW
                     VASRLQDFFGLARGPSVAAGRVPVVLHLLAPNGRAEQVTTDLAGFWDRHYPDLRRSLM
                     RRYPRHAWPDDPRTATPTAPRPRSSR"
     gene            1266..2105
                     /locus_tag="VGB85_09795"
     CDS             1266..2105
                     /locus_tag="VGB85_09795"
                     /inference="COORDINATES: protein motif:HMM:NF025158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HEY0134363.1"
                     /translation="MLTLASLLAAMTIAGAFWVGILAARRLRDWGDGRRQLAEGEGHA
                     PLALAAASSGSASSSGIMHGGLGNSLGYSGGNSLGNNGGGSGGMLHDPVSKRIRDRVA
                     QRLQGRIGPTVPRTVDVDPEAADLGLTGLRQGDVVIIETSDPALDGDYLIDGVINLRE
                     GANLTVVVVMTDADRTRWLVGSPDQDRYALCEPIRGHGLSGEPPRHIHHADQDYALER
                     RGQSSAAGVGMHGRPALPRVATYLYRAGPDQTLWLERWGEQVLMGAATSVSAHDVHFL
                     PGS"
     gene            2264..3001
                     /locus_tag="VGB85_09800"
     CDS             2264..3001
                     /locus_tag="VGB85_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134364.1"
                     /translation="MRVPAGATLTTFTVFHYPRVSWLWALQQMGSARRPLAATPGLGF
                     HHLLGSGAGLGFSRIPDLSRYALLATWSSEQAADEFFAASQLLADIRRHASDVWTVKL
                     LARRSRGSWKRRTPFTPAPDELPTGLPAVVITRASLRLRTMLRFWARVPAIERDLVAA
                     PGLRYALGVGELPWIRPITFSVWDDEAAIDRFAYAGSCHHAAARAAHTRGWFREDLFA
                     RFAAIATQGQLAGRDPCGLGPGPIIAS"
     gene            complement(2988..>4571)
                     /locus_tag="VGB85_09805"
     CDS             complement(2988..>4571)
                     /locus_tag="VGB85_09805"
                     /inference="COORDINATES: protein motif:HMM:NF045061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134365.1"
                     /translation="RIGVLGASVLLASACGDGSDGSGDSGVAATGSPITSVSAPAGET
                     GQPTTGGADGGDTTGPESTGDTSSGTTGPAAMPGPWDHGVFIPPEPQAPGDPAKGYEA
                     LLTKGYITCGIPWLLWGLAKPVLGSYANGEPMPGRPGKNAEVPHNWTVHKVDGAEIVS
                     QNCLQCHAGHFNGQLVLGLGTAEADFTQDMSAMLKLFPIPNIPIPGLEQMTRLMNRYK
                     VLGPQTVMRTVGTNPAEAVAITLVSHRDRHTLEWHDEPLLDLPNFILPSDPPPWWRAH
                     KKNALFYNGMARGDHRGTMMLATALCTDSVEEAKEIDTYFADIQAFIRSVRAPKYPFT
                     IDEALATEGEDIFNANCAGCHGSYGGPDEGDEADTYPNLLFPLEIVGTDPVVAEGAKY
                     VPELVEWYNESFYGSITRMTPDEPFRGYMAPPLDGVWATAPFLHNGSVPTIAQVLDSS
                     KRPRYWKREDFDSTNFDEDALGWPHVSLPYGHAQASDDERKYIYDTMQFAHSNVGHTF
                     GDHLTDSERGAVLEYLKTL"
BASE COUNT          587 a         1849 c         1457 g          678 t
ORIGIN      
        1 gccccgcgcg ccgcgtgttg caggtccgcg accagctgct gcgtctgtgc gagggacatg
       61 cccgaaagga caggtcacaa aacaatgcct cgttgcaggc ccgcgaggac agcctgcgtc
      121 acgccttgct ggtcgccttc ccggaccgcg tcgcccaggc ccgcgacgac gcgaccggcg
      181 gccgcgcgct cgcgctcgcg ggcggcggcg cggcgctgct gagcccggcc tcggtcgtac
      241 gcacggcccc gtggctgctc gccctcgccg tcgagcagcg ccgcgagggc gagcgcggca
      301 gccaggccgt cgtccgcagc gcctgcgcga tcgatgccga gtggctcgtc gaccacttcc
      361 ccgacgagat cgacgccatc gacctgctga ccttcaacgc cgcccgcgag cgcgtcgagg
      421 gccgctccga gctgcgctgg cacggcctgc tgatcgaggc ctcgccgcac aaaaagctcc
      481 cgcccgccgc cgcgaccgtc ctgcgagacg ccgccctcgc ggccggaccc gcgcgcttcg
      541 tcggcgaccc cgacgccctc gccggcctca tggccaggac caccttcgtc gccagccatg
      601 tccccgaatt ccccaccctc gacgacgccg ccgtccgcga cgccctcgcc gagctgtgcc
      661 acggccgcag cagcttcgcc gagctgcgcg ccgccgacct gtactgcacc ctgctcgccc
      721 gcgtcgccgg cctccacggc aacgcgctcg agcgcatggc cccgacccac atcctgctcc
      781 ccggcggccg ccgcctcgcc gtccactacg agcccggcaa gccgccgtgg gtcgcctcgc
      841 gcctgcagga cttcttcggc ctcgcgcgcg gcccgagcgt cgcggccggc cgcgtcccgg
      901 tcgtgctgca cctgctggcc cccaacggcc gcgccgagca ggtgaccacc gacctcgccg
      961 gcttctggga tcgccactac ccggacctgc ggcgcagcct catgcgtcgc tacccccggc
     1021 acgcctggcc cgacgacccg cgcaccgcga ccccgaccgc gccccgcccg aggtccagcc
     1081 gctgaccggg gccagcgtga gccgagcgcc gccgttgtcc gttgcagccg gctgttgcag
     1141 tccgcgaccc ctgtcttgca tttgcgcgag ccgtgccggc tccgcccgcg cccgggaccc
     1201 gcgcttttgc gcccggtgcc gcccctcccg cggccgcgtg gcgcgtgcta tgaaggtcct
     1261 gacgcatgtt gacgctcgcc tctctgctgg ccgccatgac catcgccggc gcgttctggg
     1321 tcgggatcct cgcagcccgg cgcctgcgcg actggggcga cggccgccgc cagctcgccg
     1381 agggcgaggg tcacgccccg ctcgcgctcg cggccgccag ctccggcagc gcgagcagct
     1441 cggggatcat gcacggcggc ctcggcaact cgctcggtta ctcggggggc aactcgctcg
     1501 gtaacaacgg cggcggctcg ggcggcatgc tgcacgaccc ggtcagcaag cggatccggg
     1561 accgcgtcgc ccagcgcctg cagggccgga tcgggccgac cgtgccgcgc accgtcgatg
     1621 tcgatcccga ggccgccgac ctcggcctca ccggcctgcg tcagggtgat gtcgtcatca
     1681 tcgagacctc cgaccccgcc ctcgacggcg actacctcat cgacggcgtc atcaacctcc
     1741 gcgagggcgc caacctgacc gtcgtcgtcg tcatgaccga cgccgaccgc acgcgctggc
     1801 tggtcggatc gcccgaccag gaccgctacg ccctgtgcga gccgatccgc ggccacgggc
     1861 tcagcggcga gcccccgcgc cacatccacc acgccgacca ggactacgcg ctcgagcgcc
     1921 gcggccagtc cagcgccgcc ggcgtcggca tgcacggccg ccccgccctc ccgcgcgtcg
     1981 ccacctacct gtaccgcgcc ggccccgacc agaccctctg gctcgagcgc tggggcgagc
     2041 aggtgctcat gggcgcggcg acctccgtct cggcccacga cgtccacttc ctccccggct
     2101 cgtgagcgac ccctccgagc caggcgcagc gcaccctccc gatgcgcccc gtccgatcgg
     2161 gcccggcatc gcaccgccgc ccgatgcgcc ccgtccgatc gcgcccgcca tcgcaccaac
     2221 gcccgacgcg ccccgtccga tcgcgcccga attcgtcacc gggatgcgcg tcccggccgg
     2281 cgccacgctg acgaccttca ccgtcttcca ctacccccgc gtctcctggt tgtgggcact
     2341 gcagcagatg ggcagcgccc ggcgcccgct cgcggccacc ccggggctcg gctttcacca
     2401 cctgctcggc tccggcgccg gcctcggctt ctcgcgcata cccgacctct cgcgctacgc
     2461 cctgctggcg acctggtcct cggagcaggc ggccgacgag ttcttcgccg cctcgcagct
     2521 cctcgcggac atccgtcgac acgcgagcga cgtctggacc gtcaagctgc tggctcgccg
     2581 cagccgcggc agctggaagc gccggacccc cttcacgccg gcccccgacg agctcccgac
     2641 cggccttccc gccgtcgtga tcacgcgcgc cagcctgcgc ctgcgcacga tgctgcggtt
     2701 ctgggcgcgc gtcccggcga tcgagcgcga cctggtcgcc gcgcccggcc tgcgctacgc
     2761 gctcggcgtc ggcgagctgc cgtggatccg cccgatcacc ttcagcgtgt gggacgacga
     2821 ggccgcgatc gaccgcttcg cctacgccgg tagctgccac cacgcggccg cccgcgccgc
     2881 gcacacccgg gggtggttcc gcgaggacct gttcgcccgc ttcgccgcga tcgccaccca
     2941 gggccagctc gcagggcgcg acccctgcgg cctcggccct ggcccgatca tagcgtcttg
     3001 agatactcga gcaccgcgcc gcgctcactg tccgtcaggt gatcgccaaa cgtgtgcccg
     3061 acgttcgagt gcgcgaactg catcgtgtca taaatatact tgcgctcgtc gtcgctcgcc
     3121 tgggcgtgcc cgtacggcaa cgacacatgc ggccagccga gggcatcctc atcgaagttg
     3181 gtgctgtcaa aatcctcgcg cttccagtag cggggccgct tgctgctgtc gagcacctgc
     3241 gcgatcgtcg gcaccgaacc gttgtgcaag aacggcgcgg tcgcccacac gccgtcgagc
     3301 ggcggcgcca tgtagccgcg aaacggctcg tccggggtca tccgcgtgat cgacccgtag
     3361 aacgactcgt tgtaccactc gaccagctcg ggcacgtatt tggcgccctc cgcgaccacg
     3421 gggtcggtgc cgacgatctc gagcgggaac aggagattgg ggtacgtgtc ggcctcgtcg
     3481 ccctcgtccg ggcctccgta acttccgtgg catccggcgc aattggcatt aaaaatatcc
     3541 tcgccctcgg tcgccagcgc ctcgtcgatc gtgaacggat acttcggcgc gcgcaccgaa
     3601 cgaatgaacg cctggatgtc ggcgaagtat gtgtcgatct ccttcgcctc ctcgacgcta
     3661 tcggtgcaca gcgccgtcgc cagcatcatc gtgccgcggt ggtcgccgcg ggccatccca
     3721 ttataaaaca gcgcgttctt cttgtgcgcg cgccaccacg gcggcgggtc ggacggcaag
     3781 atgaagttgg gcaggtcgag cagtggttca tcgtgccact cgagcgtgtg gcggtcgcgg
     3841 tgagatacca gcgtgatcgc caccgcctcg gccgggttgg tgccgaccgt gcgcatcacc
     3901 gtctgcggcc cgagcacctt gtagcggttc atcagccgcg tcatctgctc gagcccgggg
     3961 atcgggatgt tggggatcgg gaacagcttg agcatcgcgc tcatgtcctg cgtgaagtcg
     4021 gcctcggccg tccccaggcc gagcaccagc tggccattaa aatgccccgc gtggcactgc
     4081 aagcagtttt gcgaaacgat ctccgcaccg tcgaccttgt gcaccgtcca gttgtgcggc
     4141 acctcggcgt tcttgcccgg ccgccccggc atcggctcac cgttcgcata actaccgagc
     4201 accggcttcg ccagccccca cagcaaccag gggatcccac aggtgatata ccccttggtc
     4261 agcagcgcct catagccctt cgccgggtca cccggggcct gcggctccgg cgggatgaac
     4321 acgccgtggt cccacgggcc gggcatcgcc gcggggccgg tggtcccact gctcgtgtcg
     4381 cccgtgctct ccgggccggt cgtgtcacca ccgtccgcgc cgcccgtggt cggctgcccg
     4441 gtctcccccg ccggcgccga tacgctcgtg atcgggctgc ccgtggccgc gacaccgctg
     4501 tcgccgctgc cgtcgctccc gtctccgcac gcgcttgcga gcagcacgct cgcgccgagc
     4561 accccgatcc t
//
LOCUS       DASWNJ010000585         2161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_681063, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000585 DASWNJ010000000
VERSION     DASWNJ010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681063"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="VGB85_09810"
     CDS             <1..995
                     /locus_tag="VGB85_09810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0134366.1"
                     /translation="ALLVCGTLMTAIVGHLDRGGAALRSTLGRLEREISERQRIEQQL
                     RDTRDELELRVEERTIALTKINRRLEKEVRDRRGAETHALEANRIKSAFLANMSHELR
                     TPLNAIIGYCEILLEDTPKADDAALDLQKIRNSADHLLAIISDVLDLSKIEAGKMDIN
                     VEQFPVSEVVDSVMTAVSPQAEKNHNTLKLRCPRELGTIKTDRTKLHQILSNLLSNAC
                     KFTQEGRVELTVMLTFADTRRWFEFSVVDTGIGIAPETIERLFAPFTQADSSTTRKYG
                     GTGLGLAISRHYARMLGGDIHVRSTPGEGSTFTLKLPVEAVDPRSAGLLLVSHF"
     gene            complement(1014..1889)
                     /locus_tag="VGB85_09815"
     CDS             complement(1014..1889)
                     /locus_tag="VGB85_09815"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0134367.1"
                     /translation="MHRLDARLMLALLTLMSGCAGEREEGNTRDVPGSDTGAETSNGM
                     EDMGSTTAASADGGEVGHDAGGDAGMTSTSTTGDPGTTDEETTDEPQPVCGNGVLEKF
                     GRVPEECDDGNLIPDDGCDDTCAADRVMFVSSKTYQPGDFKSLYLADALCAQAANDVG
                     WANWLKFRAWLSDSKTDARDRFKLSRGRIVLVNGLVVADSWIDLLAGKLINPIEVTEK
                     GETYHGGVWTGTDPQGVGIPGATHCEDWSSFSADDVAYYGYSDRITSEWTLSELWDNP
                     APCLGDFAVYCIEDR"
     gene            complement(1923..>2161)
                     /locus_tag="VGB85_09820"
     CDS             complement(1923..>2161)
                     /locus_tag="VGB85_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134368.1"
                     /translation="EVTEKGETYHGAVWTGTNPQGVGVPGATHCEDWTSFSFDDTAYR
                     GYSDQITAEWTLSANWDNPFPCGADFAVYCIEER"
BASE COUNT          372 a          818 c          622 g          349 t
ORIGIN      
        1 cggccctgct cgtgtgcggc accctgatga ccgcgatcgt cggtcacctc gaccgcggcg
       61 gcgctgccct gcgcagcacc ctcggccgcc tcgagcgcga gatcagcgag cgccagcgca
      121 tcgagcaaca gctccgcgac acccgcgacg agctcgagct gcgcgtcgag gagcggacga
      181 tcgccctcac caagatcaat cgccgcctcg agaaggaggt ccgcgaccgt cgcggcgccg
      241 agacccacgc gctcgaggcc aaccggatca agagcgcctt cctggccaac atgagccacg
      301 agctgcgcac cccgctcaac gcgatcatcg gctactgcga gatcctgctc gaggacaccc
      361 cgaaggccga cgacgccgcc ctcgacctgc agaagatccg caactccgcc gaccacctgc
      421 tggcgatcat cagcgacgtg ctcgacctct ccaagatcga ggccggcaag atggacatca
      481 acgtcgagca gttcccggtc tccgaggtcg tcgacagcgt catgaccgcc gtctcgcccc
      541 aggccgagaa gaaccacaac accctcaagc tgcgctgccc tcgcgagctc ggcacgatca
      601 agaccgaccg caccaagctg catcagatcc tctccaacct gctctccaac gcctgcaagt
      661 tcacgcagga gggccgcgtc gagctcaccg tcatgctgac cttcgccgac acccggcgct
      721 ggttcgagtt ctccgtcgtc gacaccggca tcggcatcgc ccccgagacg atcgagcgcc
      781 tgttcgcccc gttcacccag gccgacagct cgaccacccg caagtacggc ggcaccggcc
      841 tcggcctcgc catctcgcgc cactacgcgc gcatgctcgg cggcgacatc cacgtccgca
      901 gcaccccggg cgaaggctcc accttcaccc tcaagctccc cgtcgaggcc gtcgacccaa
      961 gatccgccgg cctgctgctc gtctcacatt tctaaaaaaa gaacatcgcc gagtcagcgg
     1021 tcctcgatgc agtacacggc gaaatcaccc aggcaggggg cagggttgtc ccacagctca
     1081 gacagcgtcc actcggacgt gatgcggtcg ctgtagccat aataagcgac gtcgtcggcc
     1141 gagaacgacg accaatcctc gcagtgggtc gcgcccggta tcccgacgcc ctgcgggtcg
     1201 gtgcccgtcc acaccccacc gtgatacgtc tcccccttct cagtcacctc gatcgggttg
     1261 atcagcttcc cggcgagcag gtcgatccac gagtccgcga ccaccagccc gttgaccagc
     1321 acgatgcgac ccctgcttaa cttgaagcgg tcgcgcgcgt ccgtcttgct gtcgctgagc
     1381 cacgcgcgaa acttcagcca gttcgcccaa cccacgtcgt tcgccgcctg ggcgcacagc
     1441 gcgtccgcga ggtacaggct cttgaagtcg ccgggctggt aggtcttgct cgacacgaac
     1501 atgacgcggt ccgccgcgca ggtgtcatcg caaccatcgt ccggtatcag gttcccgtcg
     1561 tcacactcct cgggcacgcg gccgaacttc tcgagcacgc cattgccaca caccggctgc
     1621 ggttcatcgg tcgtctcctc gtccgtcgtc cccgggtcgc ccgtcgtgct cgtcgaggtc
     1681 atcccggcgt cacccccggc atcatgacca acctccccgc catcggcgct cgccgccgtg
     1741 gtcgagccca tgtcctccat gccgttggat gtctccgccc ccgtatcgct gccgggcacg
     1801 tccctcgtgt tgccctcctc gcgctcaccg gcgcagcctg acatcagcgt gagcagggcg
     1861 agcatgagtc gggcgtccaa gcggtgcatg cgtcgatctc cggtgacggg ggagggggca
     1921 tgtcagcgct cctcgatgca gtacacggcg aagtcggcac cacaggggaa ggggttgtcc
     1981 cagtttgcgg acagtgtcca ctcggcggtg atttgatcgc tgtaaccgcg gtaagcggtg
     2041 tcgtcgaacg agaacgatgt ccaatcctcg cagtgggtcg cgcccgggac tccgacaccc
     2101 tgcgggttgg tgcccgtcca cactgcgccg tggtacgtct cccccttctc cgtgacctca
     2161 g
//
LOCUS       DASWNJ010000586         1864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_435539, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000586 DASWNJ010000000
VERSION     DASWNJ010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435539"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1064
                     /locus_tag="VGB85_09825"
     CDS             75..1064
                     /locus_tag="VGB85_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134369.1"
                     /translation="MQAILLRRPLLGCLLILAACGKSTAETETAGSTGDTSTGLTSAS
                     STTANETTTPTTSTTVTTGTPETTTTDTSGVTGTSATATTTGESTSTGPDSTSEPLTS
                     SSSSSTTEPPDTEGPACITKMCNGKLWGCGDCIDNDNDGKVDAEDVECVNPCDDDEGS
                     FATGIPGDGMDACKQDCFFDGNSGAGDDKCQWNLKCDPANPGAPKCSYDANFNCPAQA
                     NMCLQVCKPPTGCDCFGCCSVYVEGELHDILLGDADCSIDNFDQCAPCTKAEDCSPEE
                     CKPELCKPCINQPLPPECMEAMCQVGEAACVTNADCPGDTEYCAAGCCKPTPQ"
     gene            complement(1074..1493)
                     /locus_tag="VGB85_09830"
     CDS             complement(1074..1493)
                     /locus_tag="VGB85_09830"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY0134370.1"
                     /translation="MTTTEPTKHTGTCFCGAIKFEVEVDASKGSRCNCRPCTKHGATT
                     SIVKPAAFTLLSDESLLFSHGNEYGRRNFCKTCGVFCFSRGHLEMIGGDYVSVNLNCL
                     DDIDPADLKLEYWDGRHDNWEAGTRDTPWPVFTGTAA"
     gene            1638..>1864
                     /locus_tag="VGB85_09835"
     CDS             1638..>1864
                     /locus_tag="VGB85_09835"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0134371.1"
                     /translation="MQAGGDRLDALVVGAGPTGLTLACELLRRGLRIRVIDSAPEASR
                     HSKALVLHVRSQEVLEQMGLAGAVQARGAAPG"
BASE COUNT          325 a          593 c          680 g          266 t
ORIGIN      
        1 tgtccggcgt gcggcgggtc cgtgttccac ggcgggcacc gggcccgtcg ccgggtccgg
       61 tgtataacga tcgcatgcaa gcgatcttac ttcggcgtcc tctcctcggt tgtctcctga
      121 tcctcgcggc ctgcggcaag agcaccgcgg agaccgagac cgccggtagc accggcgata
      181 cgtcgacggg gttgaccagc gcctcgagca ccacggcgaa cgagacgacg acgccgacga
      241 cgagcacgac ggtgaccacc gggacaccgg agaccacgac caccgacacg agcggcgtca
      301 ctggcacgag cgcgacggcg acgacgaccg gcgagagcac ctcgaccggc ccggactcga
      361 cgagcgagcc gctgacgagc tcgagcagca gcagcacgac cgagccgccg gacaccgagg
      421 gtccggcgtg cattacaaaa atgtgcaacg gcaagctgtg gggctgcgga gattgcatcg
      481 acaacgacaa cgacggcaag gtcgacgcgg aggatgtcga gtgcgtcaac ccgtgcgacg
      541 acgacgaggg ttcgttcgcg acgggcatcc cgggcgacgg catggacgcg tgcaagcagg
      601 actgcttctt cgacggcaac agcggcgcgg gcgacgacaa gtgccagtgg aacctcaagt
      661 gcgacccggc gaaccccggc gcgccgaagt gcagctacga cgcgaacttc aactgcccgg
      721 cgcaggccaa catgtgcctg caggtgtgca agccgccgac cggctgcgac tgcttcggct
      781 gctgcagcgt gtacgtcgag ggcgagctgc acgatatctt gctcggcgac gcggactgct
      841 cgatcgacaa tttcgatcag tgcgccccgt gcaccaaggc ggaagattgc agcccggagg
      901 agtgcaagcc ggagctttgc aagccgtgca tcaaccaacc gctgccgccc gagtgcatgg
      961 aggcgatgtg ccaggtcggc gaggcggcct gcgtcaccaa cgcggattgc ccgggcgaca
     1021 ccgagtactg cgcggccggc tgctgcaagc cgaccccgca gtgagcgcga agctcaggcg
     1081 gcggtgcccg tgaacaccgg ccacggcgtg tcgcgggtgc cggcctccca gttgtcgtgg
     1141 cggccgtccc agtactcgag cttgaggtcg gcggggtcga tgtcgtcgag gcagttgagg
     1201 ttgaccgaga catagtcgcc gccgatcatc tccaggtggc cgcgcgagaa gcagaacacg
     1261 ccgcaggtct tgcaaaaatt gcggcgcccg tactcgttgc cgtggctaaa cagcagcgac
     1321 tcgtcggaca gcagggtgaa ggcggccggc ttgacgatgc tggtggtcgc gccgtgcttg
     1381 gtgcacgggc ggcagttgca gcggctgccc ttgctcgcgt cgacctcgac ctcgaacttg
     1441 atcgcgccgc aaaaacaggt cccggtgtgc ttcgtgggct cggtggtggt catgcgcgag
     1501 atgatgccac acgcggatga caacatgatg tcagctattg gagtgcggct gaatttacgg
     1561 gcctcgcccg gcgatcggtg agcgcggcgc ttgcaagctg cgcccgggcc cgatacgcct
     1621 ggtgtggggg ccgagacatg caggcagggg gcgatcgact ggacgcgctg gtggtcgggg
     1681 ccgggccgac gggtctgacg ctcgcgtgcg agctgctgcg ccgcgggctg cggatccgcg
     1741 tcatcgacag cgcgccggag gcgagccggc actcgaaggc gctggtgctg cacgtgcgct
     1801 cgcaggaggt gttggagcag atgggcctcg ccggcgcggt gcaggctcgc ggcgcggcgc
     1861 ccgg
//
LOCUS       DASWNJ010000587         4734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_541128, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000587 DASWNJ010000000
VERSION     DASWNJ010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541128"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1901
                     /locus_tag="VGB85_09840"
     CDS             <1..1901
                     /locus_tag="VGB85_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0134372.1"
                     /translation="HEIERYVAAEGKPPKLDKMGGTTFLVKAGKVKQEVRQMAEELLQ
                     IYAQRESQPGVAYPVLREEVEAFADTFPYAETADQAEVISAVQGDLSRTRPMDRLVCG
                     DVGFGKTEVALRAAFRVAMAGKQVAVLAPTTVLVQQHYLTFSDRMRPFPIKVAVLNRF
                     ASDRQQKQVVEAVREGKVDIVIGTHRMLGRDVRFKDLGLLIIDEEQRFGVAQKERFKK
                     FKSQVDVLTLSATPIPRTLHMSLLGLREISMIRTPPVDRLAVRTYITRISDAVIEEGV
                     TREMARGGQVFYVVPRILGIEEHANRIRQLVPSARVLVAHGQVPDEMLERTMVDFVEH
                     RADVLVSTAIIESGLDIPRANTMFIAHAEQFGLAQIYQLRGRVGRSKQRAHCYLMVQA
                     IERLSPEARRRLEAVQRHSELGSGFGVAVHDLEIRGAGELLGARQSGQIQAIGFDAYA
                     RILQEAVAELRGQPITRETDPEMVFDAPAYLPESYVSEVGQRLDFYRRLSAARDRDQV
                     DDVIAELVDRFGDPPLEAEHLGLLMACKTYGRRLGALALELRGTKLGVRLGPETPLTG
                     EVAAGLNKRTSGKFRLESGERVTSTVPEPTGPDRRNQLRMCLAALVELTAIAEAAVHK
                     GLGARKAG"
     gene            2000..2971
                     /locus_tag="VGB85_09845"
     CDS             2000..2971
                     /locus_tag="VGB85_09845"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0134373.1"
                     /translation="MRRLSLALSLFAVAAAAAWPGVASAELLDRVVAVVNDEVVLQSE
                     LEQALRGSPELQERLSQLGPSATPQQAEQVMREERAKTLNDLIKNVLVKKEAQRFQIE
                     VNEADVDRYLQQLAQQNQFASIAELRKAVEESGEYGAWSDYRQDIRDQLIVFKTTQSL
                     ANFAVTDAAVREQYRKMTRGEESKVEVERFLFGADEQTAAARDAAFTKAQVTARRLRS
                     GEAVEAVATEYGLAGKTHKEYARGEVAPAVEDAIFAAGPGAVVGPLETGQGYLVLKIV
                     QHVASDVLSFEQAKDKIRDGLEQEAFAKAAEDFRQGLLAKAHIDIRL"
     gene            3071..4438
                     /locus_tag="VGB85_09850"
     CDS             3071..4438
                     /locus_tag="VGB85_09850"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0134374.1"
                     /translation="MTGPVQEALDDWSLLLAVTGRLAAAETIESTLVALVSPAPAATE
                     AGACLWTVDCEADGTPSWLTLVGSLPMAARPARAEVGARERVPETTLAGLCLTHPGVP
                     VLIPSVDGDARLDAHVRELGRALGITAAIMLSLALRGRIVGLLTIQWARPVALGAREQ
                     RVYQALACHVALALDNLVMTDRMGCLLAESQRERRLLSTVLEHVPVGILCIAGPTRRP
                     LLTNRMGRLMLTGDPGPVSEALPLAHMLYPDTDEPICAEDLAGVRAARTGQRQERELD
                     LIPPGGSRISVVTIGVPVLDADGKVDQVVVVMNDITGRKRAAQERAQLQEAVIQAQAA
                     ALVERSTPLIPITDDILVMPLIGLIDDARGQGILDVALQGARERRARVTILDVTGVPS
                     IDAQAAAVLTRTAQALRLIGVVPVLSGVCPAVSRALVELDVPLTGIVTCGSLQAGIKY
                     AMRRR"
     gene            complement(4466..>4734)
                     /locus_tag="VGB85_09855"
     CDS             complement(4466..>4734)
                     /locus_tag="VGB85_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134375.1"
                     /translation="TRPLVLNESEQYGVIVWPHKLLVRPGDNLTELYDLSEDFAEARN
                     LAADAPRRVGELMQYYHAAPSVNLDRTSRGRRLRERAAVNDGGE"
BASE COUNT          692 a         1589 c         1776 g          677 t
ORIGIN      
        1 tgcacgagat cgagcgctac gtcgcggccg agggcaagcc gcccaagctc gacaagatgg
       61 gcggcacgac ctttttggtc aaggccggca aggtcaagca ggaggtccgc cagatggcgg
      121 aggagctgct gcagatctac gcccagcgcg agagccagcc cggcgtcgcg tatccggtgc
      181 tgcgcgagga ggtcgaggcc ttcgccgaca ccttcccgta cgcggagacg gccgaccagg
      241 cggaggtcat ctcggcggtg cagggcgatc tttcacgcac gcggccgatg gaccgcctcg
      301 tgtgcggcga cgtcggcttc ggcaagacgg aggtggcgct gcgcgccgcg ttccgcgtgg
      361 cgatggcggg caagcaggtc gcggtgctcg ccccgacgac ggtgctcgtg cagcagcact
      421 acctgacgtt tagcgaccgc atgaggccgt tcccgatcaa ggtcgcggtg ctcaaccgct
      481 tcgccagcga ccgacagcag aagcaggtcg tcgaggcggt gcgcgagggc aaggtcgaca
      541 tcgtcatcgg cacgcaccgg atgctcggca gggacgtccg cttcaaggat ctcggactgc
      601 tgatcatcga cgaggagcag cgcttcggcg tcgcccagaa ggaacgcttt aaaaagttca
      661 agtcgcaggt cgacgtgctg acgctgtcgg cgacgccgat cccacgcacg ctgcacatgt
      721 cactgctcgg cctgcgcgag atcagcatga tccgcacgcc gccggtcgat cgcctggcgg
      781 tgcgaacgta catcacgcgg atctccgacg cggtgatcga ggagggcgtc acgcgcgaga
      841 tggcccgcgg cggccaggtg ttctacgtgg tgccacggat cctcggcatc gaggagcacg
      901 ccaaccggat ccgccaattg gtgccgagcg cccgcgtact ggtcgcccat ggccaggtgc
      961 ccgacgagat gctcgagcgc acgatggtcg acttcgtcga gcaccgcgcc gatgtcctgg
     1021 tgtcgacggc gatcatcgag agcggcctcg atattccccg cgccaacacg atgttcatcg
     1081 cccacgccga gcagttcggc ctggcccaga tctaccagct gcgcggccgc gtcggccgga
     1141 gcaagcaacg cgcgcactgc tacctgatgg tccaggcgat cgagcgcctg tcgcccgagg
     1201 cgcgacggag gttggaggcg gtgcagcgcc acagcgagct cggctcgggc ttcggcgtcg
     1261 cggtgcacga cctcgagatc cgcggcgccg gtgagttgtt gggagcgcgc cagagcgggc
     1321 agatccaggc gatcggcttc gacgcgtacg cgcggatcct gcaggaggcg gtcgccgaat
     1381 tgcgcgggca gccgatcacc cgcgagaccg accccgagat ggtgttcgac gcgccggcgt
     1441 acctgcccga gagctacgtg tcggaggtcg gccagcgcct cgatttttat cgcaggctgt
     1501 cggccgcccg cgaccgcgac caggtcgacg acgtgatcgc cgagctcgtc gaccgcttcg
     1561 gcgacccgcc gctcgaggcg gagcacctcg gcctgctgat ggcctgcaag acctacggcc
     1621 ggcggctggg cgcgctcgcg ctcgagctgc ggggcaccaa gctcggcgtg cgcctcgggc
     1681 ccgagactcc gctgactggc gaggtcgcgg cggggctcaa caagcgcact tcgggcaagt
     1741 tcaggctcga gagcggggag cgggtgacga gcacggttcc ggagccgacc gggccggacc
     1801 ggcgtaacca gctgcgcatg tgcctggcgg cgctcgtcga gctgactgcg atcgccgagg
     1861 cggcggtgca caaggggctc ggggcgcgca aggcgggctg aaagaggccg cggcggatct
     1921 gtcgccgacg gcgcagcgcg cccatttgtg gcccggtcgc ttgcctgcgc gggtgcgccg
     1981 ctggtatgcc tacggggcca tgcgtcgcct gtctctcgcc ctcagcctgt tcgccgtcgc
     2041 cgctgccgcc gcatggccgg gcgtggcctc cgcggagctg ctggaccggg tggtcgcggt
     2101 ggtcaacgac gaggtcgtgc tgcagagcga gctcgagcag gcgctgcgcg gctcgccgga
     2161 gctgcaggag cgcctgagcc agctcgggcc gagcgcgacg ccgcagcagg ccgagcaggt
     2221 catgcgcgag gagcgggcca agacgctcaa cgacctgatc aaaaatgtgc tggtcaagaa
     2281 ggaggcgcag cgcttccaga tcgaggtcaa cgaggcggac gtcgatcgct acctgcagca
     2341 gctcgcgcag cagaatcagt tcgcgagcat cgccgagctg cgcaaggcgg tcgaggagtc
     2401 gggtgagtac ggggcctggt cggactaccg ccaggacatc cgcgaccagc tgatcgtctt
     2461 caagaccacg cagtcgctcg ccaacttcgc cgtcaccgac gcggcggtgc gcgagcagta
     2521 ccgcaagatg acgcgcggcg aggagtcgaa ggtcgaggtc gagcgcttcc tgttcggcgc
     2581 cgacgagcag accgctgccg cccgcgacgc ggcgtttacc aaggcccagg tgaccgcgcg
     2641 acgcctgcgg tccggcgagg cggtcgaggc ggtcgccacc gagtacggcc tcgccggcaa
     2701 gacccacaag gagtacgcgc gcggcgaggt cgccccggcc gtcgaggacg cgatcttcgc
     2761 ggccggcccc ggcgcggtgg tcggcccgct cgagaccggc cagggctacc tggtcctcaa
     2821 gatcgtgcag cacgtcgcct ccgacgtcct cagcttcgag caggccaagg acaagatccg
     2881 cgacgggctc gagcaggagg cgttcgccaa ggccgccgag gactttcgtc aggggctgct
     2941 ggcgaaggcg cacatcgata ttcgcctgtg agtcggcccg tccgggccca tccgtgcccg
     3001 cgggcccggg cggccgcgcg ccgacctctg tgcgcagaga gacggcgccg atgttagcgt
     3061 gcagcctggt atgacgggcc cggtgcagga ggcgctcgac gactggtcgc tgctgctggc
     3121 ggtcaccggg cggctcgccg cggcagagac gatcgagtcg acgctggtgg ccctcgtcag
     3181 cccggcgccc gcggcgacgg aggccggggc gtgcctgtgg acggtcgact gcgaggccga
     3241 cgggaccccg tcgtggctga cgctggtggg ctcgctgccg atggcggccc gaccggcgcg
     3301 cgcggaggtc ggcgcgcgtg agcgcgtgcc ggagaccacg ctcgccggcc tgtgcctgac
     3361 tcacccgggt gtgccggtgt tgatcccgtc ggtcgacggt gacgcgcgcc tcgacgcgca
     3421 cgtgcgcgag ctggggcggg cgctcggcat cacggcggcg atcatgctgt cgctggcgct
     3481 gcgcgggcgg atcgtcgggc tgctgacgat ccagtgggcg cggccggtcg cgctcggggc
     3541 tcgtgagcag cgggtgtatc aggcgctggc ctgtcatgtg gcgctcgcgc tcgacaacct
     3601 cgtgatgacg gatcgcatgg gctgcctgct ggccgagtcg cagcgggagc gccgactgct
     3661 gtcgaccgtg ctcgagcacg tgccggtcgg catcctgtgc atcgcggggc cgacgcggcg
     3721 gccgctgctg accaaccgca tgggtcgcct gatgctcacc ggcgacccgg ggccggtctc
     3781 cgaggcgctg ccgctggcgc acatgctgta cccggacacc gacgagccga tctgcgcgga
     3841 ggacctggcc ggcgtgcggg cggcgcggac tggccagcgg caggagcgcg agctcgacct
     3901 catcccgccg ggcgggtcac ggatctcggt cgtgacgatc ggcgtgccgg tgctcgacgc
     3961 cgacggcaag gtcgatcagg tggtcgtcgt catgaacgac atcaccggcc gcaagcgggc
     4021 ggcccaggag cgagcgcagc tgcaagaggc ggtgatccag gcgcaggcgg cggcgctggt
     4081 cgagcgcagc acgccgctga tcccgatcac ggatgacatc ctggtgatgc cgctgatcgg
     4141 gctgatcgac gatgcgcgtg gccaggggat cctcgacgtc gccttgcaag gcgcgcgcga
     4201 gcgccgggcc cgtgtgacga tcctcgacgt caccggcgtg ccgtcgatcg acgcgcaggc
     4261 ggccgccgtg ctgacccgca ccgcgcaggc gctgcggctg atcggggtcg tgccggtgct
     4321 cagcggagtc tgcccggccg tgtcccgggc gctggtcgag ctcgacgtgc cgctgacggg
     4381 gatcgtgacc tgcgggtcgc tccaggccgg catcaagtac gcgatgcgtc ggcgctagcg
     4441 atgctggcga tgccgaagcg gctcgctact cgccgccatc gttcaccgcg gcgcgctcgc
     4501 gtagccggcg gccgcggctc gtacggtcga ggttcacgct cggcgccgcg tggtagtact
     4561 gcatcagctc gccgacgcgg cggggcgcgt ccgcggcgag gttgcgggcc tccgcgaagt
     4621 cctccgagag gtcgtacagc tcggtgaggt tgtcgcccgg tcgcacgagc agcttgtgcg
     4681 gccacacgat gacgccgtac tgctcgctct cgttgagcac cagcggacgg gtgc
//
LOCUS       DASWNJ010000588         2248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_646553, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000588 DASWNJ010000000
VERSION     DASWNJ010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646553"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="VGB85_09860"
     CDS             complement(<1..1048)
                     /locus_tag="VGB85_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134376.1"
                     /translation="MSSSVAVGSRLLWIPLLVCGCGLFGDGNDGEDGDGNDGGRDEGG
                     VEDGQLTDSTPTEGGWDAGGWDVGGEGGPDGGWEGGSDSQPTTTGIEPTGADNELPEG
                     DQCPCADDNEDIYVLSDIGTIWKFDPQNNQFDFITDVECGGMTDTFSMGVSRKARAWV
                     QYFGGDLYTIDLKDPGSACKDPGFENDEPLFPNFGMAFVANSATDPCDRLYAHSGIDP
                     ALIGEDVGALGVIDPITLELSEIAKIDFAWGELTGTGTGRLYAFQGSSPPFISEYDKT
                     TGAILGSWPLPGLDSPDAFAFAAWGGDFYLFMTSGDDFLGNSRVVHVDFDESDGDGMA
                     ITTVVEQAPIRIVGA"
     gene            1445..1837
                     /locus_tag="VGB85_09865"
     CDS             1445..1837
                     /locus_tag="VGB85_09865"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0134377.1"
                     /translation="MTENEVAQEIGRRIRHWRTSTPPPKTRRKRQNETGWRPHLTIEE
                     LAKATGLTVTTVNKLELGYKSPRIESLLLLAQAFGCEPSELLPRNKHRNSGKAELLSE
                     LGTAAAQLGAKQLRELIKAARALEPTSG"
BASE COUNT          344 a          759 c          840 g          305 t
ORIGIN      
        1 cggcgccgac gatgcggatc ggcgcctgct cgacgaccgt cgtgatcgcc atgccgtcgc
       61 cgtcgctctc gtcgaagtcg acgtggacga cgcggctgtt gccgaggaag tcgtcgccgc
      121 tggtcatgaa caggtagaag tcgccgcccc aggccgcgaa ggcgaaggcg tccgggctgt
      181 cgaggccggg caggggccag ctgccgagga tggcgccggt cgtcttgtcg tactcgctga
      241 tgaacggggg gctggagccc tggaacgcgt acagccggcc ggtgccggtg ccggtcagct
      301 cgccccacgc gaagtcgatc ttggcgatct cgctcagctc gagggtgatc gggtcgatga
      361 caccgagcgc gccgacatcc tcgccgatca gcgccgggtc gatgccggag tgcgcgtaga
      421 gccggtcgca cgggtcggtg gcgctgttgg cgacgaaggc catgccgaag ttggggaaca
      481 gcggctcgtc gttctcgaag ccggggtcct tgcaggccga gccggggtcc ttgaggtcga
      541 tggtgtacag gtcgccgccg aagtactgca cccacgcgcg ggccttgcgg ctgacgccca
      601 tcgagaaggt gtcggtcatg ccgccgcact cgacatcggt gatgaagtcg aactgattgt
      661 tctgcggatc gaacttccag atcgtgccga tgtccgagag cacatagatg tcctcgttgt
      721 cgtcggcgca cggacactgg tcgccctcgg gcagctcgtt gtcggcgccg gtcggctcga
      781 tgccggtggt cgtcggctgg ctgtcgctgc cgccctccca gccgccgtcg ggcccgccct
      841 cgccgccgac gtcccagccg cccgcgtccc agccgccctc ggtcggggtg ctgtcggtga
      901 gctggccgtc ctcgacgccg ccctcgtcgc gaccgccgtc gttgccgtcg ccgtcctcgc
      961 cgtcgttgcc gtcgccgaac aagccgcagc cacagacgag cagcgggatc cagagcagac
     1021 gcgacccgac ggcgacggac gaggacatct cgtcagcgta gcgcaggcac ccgggcgtgt
     1081 gcgcccaccg gcgccggatc gtccacacgc gcagcagcgg cccgcaaaac gctgaaccgg
     1141 cgcggccctc ggccgggccg accgcgtggc cgggtccggg ggcggtcgct tcgcgcggac
     1201 gatcgggacg tcgtcgacgc gacggcggtg cggagcgtgc ggatggtgcg gatggtgcgg
     1261 ggccgaggag gcacgcgggg tccggtgcgc gcctgcccca aaggccatct cgggcgacca
     1321 cgaagcgacc ggacccaagg gccaggtcgc cccgcctttg ggacaggtcc cgagagcaag
     1381 ataggcgctt gccggggggc gggctcgcct gtagagttcg ggcctccacc tccagcgacg
     1441 gaccatgaca gaaaacgaag tagcccagga aatcggacga cggatcaggc actggcgcac
     1501 gtcgactccg ccgccgaaga cccgccgcaa gcgccagaac gagaccggct ggcggccgca
     1561 cctcacgatc gaggagctgg cgaaggcgac cggactgacg gtgacgaccg tcaacaagct
     1621 cgagctcggc tacaagtcgc cgcgcatcga gtcgctgctg ctgctggccc aggcgttcgg
     1681 ctgcgagccg agcgagctgc tgccgcgcaa caagcaccgc aactcgggca aggcggagct
     1741 gctctcggag ctcggcaccg ccgccgcgca gctcggcgcg aagcagctgc gcgagctgat
     1801 caaggccgca cgcgcgctcg agccgacgtc cggctaaaca cgatcgcggg gcgcagacgt
     1861 cccgcggtgc gcgtctcgcc gtcgatcaca ttcgcggatc ggcggcggtc gatgaaggcg
     1921 tgagggcgtg aaggcgttgc ggccgccgct tgtcaggccc gggggctcgc attagtgttc
     1981 gatcgtggca aaacgacgat ggggcggagc ggcgtattgg ctggtcgcgc tggtgaccgc
     2041 tggctgtgcg gacgatcctg cgggtaccgc tggtgcgacg atgggcggca cggggacgac
     2101 cggcacgacc gggacgggtg acgcgacggc gcagggctcg acgccgacca ccggctcggg
     2161 cgcggagacc ggcagcgccg tcacgagcga gggcgcgagc gcgaccgagt cgacgccgac
     2221 caccggtcca ggcacgggcg cgacgagc
//
LOCUS       DASWNJ010000589         6471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_116770, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000589 DASWNJ010000000
VERSION     DASWNJ010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6471
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116770"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1272)
                     /locus_tag="VGB85_09870"
     CDS             complement(<1..1272)
                     /locus_tag="VGB85_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134378.1"
                     /translation="MDNKRKGSDPQQSPSESDRFEWDKPQPNRSRDSFNFADDDEDGP
                     VPAPPEFDGERGSVPDLLDGEEVVEEPIEDSDLLESAPEDDDDARHSHHHHHHHGPPP
                     IPVGASQRGFIDDGEIALPPRMSHLVKSFPFRHVRAEDDPSAEDNLDDEPEEMEPEEL
                     DPDDLESVTDGAIAGRSIAGQYIAGQSIAGRSRDPFADDDTDDVARTSDPATGTGARW
                     SFADLDRQSAASTPAPAPAKSWSDDEDTVAEPLSAASTLTSAGRARMFSDDSERTVAD
                     SATDDDTGIFTDVPDNTPTGLFARNDLRAAAVDPPRPAKNPAFASLPTDDELELPIDD
                     DAPPSRRPLETAPVDDDESTAMFTARRLRDAADSVSRAAELQGEDDWVHPRGPQPSAA
                     IPVADIDDDDDASWDLPPRPPEPVAAPEPAAV"
     gene            complement(1444..2364)
                     /locus_tag="VGB85_09875"
     CDS             complement(1444..2364)
                     /locus_tag="VGB85_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0134379.1"
                     /translation="MLRLAPAAGWFLAPTSLVTSSGLACLITFLVACSGPEAPPASKL
                     SETKNLIQQPTPARKDIPSPFVNPNKEPPPPRSEPQVPPAELASALARSEAARKIGDD
                     LEASLALRACANKVPQSVQCEGELAIVLAKMPRHKYEAEYYLKQAIAADDPALTADYY
                     RRLGEALYKKGMFLESITAYERMVTRTPKPTADDYNQLAVALQAAPERVLDAAEALRK
                     AYELDGTRHEWLRDQAILLGQVPNKIAQAIVLFQELRGKVTDPVLVADTDRRIADLQA
                     VLAAGTGTAAPAKQPAGPQKPLKKPAPPTR"
     gene            2387..2917
                     /gene="coaD"
                     /locus_tag="VGB85_09880"
     CDS             2387..2917
                     /gene="coaD"
                     /locus_tag="VGB85_09880"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEY0134380.1"
                     /translation="MSASAIHDPSGPPHPLTAVYPGSFDPITRGHVEIVERALRIFDR
                     VVVAVGRHPSKPGFFPVEARCELISASIAHLPRASAEHFEGLVIDFCRRRGASAILRG
                     LRAHGDFEPEFQMALANRDLAPEIETVFMIPQPAQQFVSSSLIREIAHHGGDFERYVP
                     PAVAEAMHRRLGRGEA"
     gene            2914..3537
                     /locus_tag="VGB85_09885"
     CDS             2914..3537
                     /locus_tag="VGB85_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134381.1"
                     /translation="MSDDAKKDGPLVRTGEPWRGDEHKLQVLAAQEAGLAPTQVAVDL
                     YTEGFKAGLQERDPADGAIVLRGRTVLKADELERSLRPMLRFLRVGPDAARVRVFDGQ
                     FELRIAAAAAEAGAAVQGAARAVLLMWMAGALLGWWMLSVSRVATFLIWGVTLLTGAW
                     SLRRGLVSGRTMLAARLAVGLGMLAHEEQLILPPADGSSRGGAGGTS"
     gene            3763..5001
                     /locus_tag="VGB85_09890"
     CDS             3763..5001
                     /locus_tag="VGB85_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134382.1"
                     /translation="MIPTTSSRFMLPGFALVAGLVVMSGTARAGNGIHPRTPVKWEPE
                     PACMTIVDRSVDAKLTFNYSIPYEDTRPDNAVDEVEDSRRHQFIAFCHGHSVQQPLPV
                     WLSMADVDAAKGVGIIDMGGVAAEDILELNPEWKDCFTRITADDARREILFAEANKPV
                     VWDTTGLAAGAYIVSGYTWEPVFNIWSVRPGVVKVVDDPDPAKNGPALAITNRNEVKY
                     GDEVLMLTGCVDAMDGSTITGYWSLTDNADVLDWKPFAAGTPVSGDSFELPFMAPPEA
                     VGGPVTIKIEITDPMDRKFTAHMLTLADFLQGSAGESGDCSDTGNFIGMPGCEGSDDG
                     GGTTGGDSSGPGVTSTGTPDTTPTTAATGTTGEGGSSEETGTTPQTGEGSCGGCTLGG
                     GGVPAVLLGPWLLWATRRRR"
     gene            5118..5771
                     /gene="mtnB"
                     /locus_tag="VGB85_09895"
     CDS             5118..5771
                     /gene="mtnB"
                     /locus_tag="VGB85_09895"
                     /EC_number="4.2.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylthioribulose 1-phosphate dehydratase"
                     /protein_id="HEY0134383.1"
                     /translation="MSAGRQESIPELICELCRQFYDLGWVSGTGGGISIRGAEGIYIA
                     PSGVQKERMRPEDIYLLDEKDLDDAKVVCAPADGSLKISECRPLFYNAYRLRNAGAVL
                     HSHSIWSVLASRMFSNEFVCQGLEMQKGLRGVGCFDTLRVPIIANTAREAELTASMAA
                     AIEGYPQVDAVLVAGHGVYVWGATWAQAKTQAECYDFLFRAVVEAHRLGIRLTTTPA"
     gene            5784..6239
                     /locus_tag="VGB85_09900"
     CDS             5784..6239
                     /locus_tag="VGB85_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0134384.1"
                     /translation="MHLSWLESDASTLSSAPIDTADLNAAGVLYERLGLEDYQASLER
                     LKAERGYVTQDIVELHPEMPNLQAVCDKFKDEHLHRDDEVRFVLAGEGIFDIRAKDGR
                     WMRVQVEPGDLIVVPADLYHRFFLTERQQIRCVRLFKDAAGWVPEYRVA"
     gene            6291..>6471
                     /locus_tag="VGB85_09905"
     CDS             6291..>6471
                     /locus_tag="VGB85_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY0134385.1"
                     /translation="MSASTVTKIHPEQTQDFKVADMSLAEWGRKEIELAEKEMPGLMS
                     LRSKYGAEKPLKGAKI"
BASE COUNT          924 a         2025 c         2501 g         1021 t
ORIGIN      
        1 gacagcggcg ggctcgggcg cggcgaccgg ctcgggcggg cgcggcggga ggtcccagct
       61 ggcgtcgtcg tcgtcgtcga tgtcggcgac ggggatcgca gcggagggct gcgggccgcg
      121 ggggtgcacc cagtcgtcct cgccctgaag ttccgccgcg cgcgacacgg agtcggcggc
      181 gtcgcgcagg cgccgggcag tgaacatcgc ggtcgactcg tcgtcgtcga ccggcgcggt
      241 ctccagcgga cggcgcgagg gcggagcgtc gtcgtcgatc gggagctcga gctcgtcgtc
      301 ggtcggcagc gaggcgaagg ccgggttctt cgcggggcgc ggagggtcga ccgcggcggc
      361 gcggaggtcg ttgcgcgcga acaggccggt cggcgtgttg tcggggacgt cggtgaagat
      421 gccggtgtcg tcgtcggtcg cgctgtcggc gacggtgcgc tcgctgtcgt cgctgaacat
      481 gcgcgcgcgg ccggccgagg tcagcgtgct cgcggcgctg aggggctcgg cgacggtgtc
      541 ttcgtcgtcg ctccacgact tcgcgggcgc gggcgcgggg gtcgacgcgg cgctctggcg
      601 gtcgaggtcg gcgaagctcc agcgcgcgcc ggtgccggtg gcggggtcgc tggtgcgcgc
      661 gacatcgtcg gtgtcatcat ccgcgaaggg gtcacgggat cggccggcga tggactggcc
      721 ggcgatgtac tggccggcga tggatcggcc ggcgatcgca ccatcggtga cggactcgag
      781 gtcgtcgggg tcgagctcct cgggctccat ctcctcgggc tcgtcgtcga ggttgtcctc
      841 ggcgctgggg tcgtcctcgg cgcggacgtg ccggaacggg aagctcttga cgaggtggga
      901 catccgcggc ggcagggcga tctcgccgtc gtcgatgaag ccgcgctgac tcgcgccgac
      961 cgggatcggc ggcgggccat gatgatgatg atgatgatgg gaatgacgcg cgtcgtcgtc
     1021 gtcttccggg gcggactcga gcaggtcgga gtcttcgatc ggctcctcga cgacctcctc
     1081 gccgtcgagc agatcgggga cgctgccgcg ctcgccatcg aactcgggag gcgcgggcac
     1141 ggggccgtcc tcgtcgtcgt cggcgaagtt gaacgaatcg cgagacctgt tcggctgggg
     1201 cttgtcccac tcgaagcggt cgctttcgga gggcgactgc tgcggatccg atcccttgcg
     1261 cttgttgtcc acgggcggcc cgagagtcgc atagcgcccg tggcaggtca aaccggcgca
     1321 ggtcggcgat tggcggtcat ttcgaggtcg gggggtcccg gagggcgccg gagggggtgc
     1381 ggcgtgcgtg tgcgccgggg caccgggcgg ggttcgccgg gcatgcgcgg gtaccgtggg
     1441 gggtcagcgg gtcggcggag ccggcttctt gagcggcttt tggggccccg cgggctgctt
     1501 tgccggggcc gccgtgccgg tccccgcggc gagcaccgcc tgaagatcgg cgatccgccg
     1561 gtcggtgtcc gcgacgagca cggggtcggt gaccttgccg cggagctcct ggaacaggac
     1621 gatcgcctgc gcgatcttgt tcggtacctg gccgagcagg atcgcctggt cgcgcaacca
     1681 ctcgtggcgg gtgccgtcga gctcgtaggc cttgcgcagg gcctcggcgg cgtcgagcac
     1741 ccgctcgggc gcggcctgga gggcgaccgc gagctggttg tagtcgtccg cggtcggctt
     1801 cggggtgcgg gtgaccatgc gctcgtaggc ggtgatcgac tcgaggaaca tgcccttttt
     1861 gtagagcgcc tcgccgaggc ggcggtagta gtcggcggtc agcgcggggt cgtctgcggc
     1921 gatcgcctgc ttcaaatagt actcggcctc gtacttgtga cgcggcatct tggcgagcac
     1981 gatcgccagc tcgccctcgc actggacgct ctgcgggacc ttgttggcgc aggcgcgcag
     2041 cgccagggag gcctcgaggt cgtcgccgat cttgcgcgcg gcctccgagc gcgcgagcgc
     2101 cgaggcgagc tcggcgggcg gcacttgcgg ctcgctgcgc ggcggcggcg gctccttgtt
     2161 ggggttgacg aagggcgagg ggatgtcctt gcgggccggg gtgggctgct ggatgaggtt
     2221 cttcgtctcg ctcagcttgg aggcgggcgg cgcctccggg ccggagcagg cgacgaggaa
     2281 ggtgatgagg caggccaggc cgctggatgt cacgagcgag gtgggcgcga gaaaccagcc
     2341 ggcggcgggt gcaaggcgga gcatggtccg gattatactc ccgtccgtga gcgcgtccgc
     2401 catccacgac ccgtccggtc cgccgcatcc gctgaccgcg gtgtatccgg gctccttcga
     2461 cccgatcaca cgcggtcacg tggagatcgt cgagcgagcg ctacggatct tcgaccgcgt
     2521 ggtggtcgcg gtcggccggc acccgagcaa gccgggcttt ttcccggtgg aggcccgctg
     2581 cgagctgatc tcggcgagca tcgcccactt gccccgggcc agcgcggagc acttcgaggg
     2641 cctggtgatc gacttttgtc gccggcgcgg ggcgtcggcg atcttgcggg gattacgagc
     2701 ccacggggac ttcgagccgg aattccagat ggccctcgcc aaccgcgacc tcgcgccgga
     2761 gatcgagact gtcttcatga tcccgcagcc ggcccagcag ttcgtgtcga gctcgctcat
     2821 ccgtgagatc gcccaccatg gcggtgactt cgagcgctac gtgccgcccg cggtcgccga
     2881 ggcgatgcac cgccgactcg gcaggggcga ggcgtgagcg acgacgcaaa gaaggatgga
     2941 ccgctggtcc gcaccggcga gccgtggcga ggcgatgagc acaagttgca ggtgctcgcg
     3001 gcccaggagg ccgggctggc gccgacgcag gtggcggtcg acctttatac ggagggcttc
     3061 aaggccggct tgcaggagcg tgacccggcc gacggggcga tcgtgcttcg cggtcgcacg
     3121 gtgctcaagg ccgacgagct cgagcggagc ctgcgaccga tgctgcggtt tcttcgggtc
     3181 ggcccggacg cggcgcgcgt gcgcgtgttc gacgggcagt tcgagctgag gatcgcggcg
     3241 gcggcggccg aggccggggc ggcggtgcag ggggcggcgc gcgcggtgct gctgatgtgg
     3301 atggccgggg cgctgctcgg ctggtggatg ctgtcggtgt cgcgggtggc gacgttcctg
     3361 atctggggcg tgaccttgct gaccggggca tggagcttgc ggcgcgggct ggtcagcggg
     3421 cggacgatgc tggcggcgcg cctcgcggtc ggcctcggca tgctcgcgca cgaggagcag
     3481 ctgatcttgc cgccggcgga tggctccagc cgtggcggcg cgggcggcac gtcgtgaatt
     3541 cacccgatcc agggctgtgt cgcgggtgcc ggcacgcgca cgtggtcgtc agcgggcgcg
     3601 ggtcgagctt ttggcgctgc aaggtgcacg acgtggagcc gaggtggccg aagtatccgc
     3661 cgctgccggt gctgcgctgc gctcgcttcg cggcgaacgc ggagggcggg ggcgacggag
     3721 gacttacgcc gccgccgaac cggatgtaga tttccggtga tgatgatccc gacgacgtcg
     3781 agccgcttca tgctccccgg gttcgcgctg gtcgcgggtc tcgtcgtgat gtccgggacg
     3841 gcgcgggcgg gcaacggcat tcatccgcgc acgccggtca agtgggagcc ggagccggcg
     3901 tgcatgacga tcgtcgatcg ttcggtcgac gcgaagctga ccttcaatta ttcgatcccg
     3961 tacgaggaca cgcggccgga caacgcggtc gacgaggtcg aggacagccg gcgccaccag
     4021 ttcatcgcgt tttgccacgg tcacagcgtg cagcagccgc tgccggtgtg gttgtcgatg
     4081 gccgacgtcg acgcggccaa gggggtcggg atcatcgaca tggggggcgt cgcggcggag
     4141 gacatcctcg agctcaatcc ggagtggaaa gattgcttca cgcggatcac ggccgacgac
     4201 gcgcggcgcg agatcctgtt cgccgaggcg aacaagccgg tggtgtggga caccacgggg
     4261 ctggccgcgg gcgcgtacat cgtgtccggg tacacgtggg agccggtgtt caacatttgg
     4321 agcgtccgtc ccggggtggt caaggtggtc gacgatccgg atccggccaa gaatggaccg
     4381 gcgctggcga tcaccaaccg caacgaggtc aagtacgggg acgaggtcct gatgttgacc
     4441 ggctgcgtcg acgcgatgga cggctcgacg atcacgggct actggtcgct gaccgacaac
     4501 gccgacgtgc tcgactggaa gccgttcgcc gccggcacgc cggtcagcgg cgacagcttc
     4561 gagctgccgt tcatggcgcc gcccgaggcc gtcggcgggc cggtcacgat caagatcgag
     4621 atcaccgatc cgatggaccg caagttcacc gcgcacatgt tgacgctcgc ggacttcctg
     4681 cagggcagcg cgggcgagag cggcgattgc tcggacaccg ggaacttcat cggcatgccc
     4741 ggctgtgagg gcagtgacga cgggggcggc acgaccggcg gcgacagcag cggcccgggt
     4801 gtcacgagca cgggcacgcc ggacacgacg ccgacgaccg ccgcgacggg gaccaccggc
     4861 gagggcggca gcagcgagga gaccgggacc acgccgcaaa ccggcgaggg cagctgcggc
     4921 ggttgtacgc tcgggggggg cggtgtgccc gcggtgttgc tcggtccgtg gctgctgtgg
     4981 gcgacgcgac gacgccgcta gacgagacga agaagaagcg agcgcgcagg tgcgaggatc
     5041 cgcactgctg gggcctaccc ccggttctga ggtacgggag tacactcccg tgccccgcac
     5101 gccggggtga acgtcgcatg tccgcaggcc gccaagaatc gatccccgag ctcatctgtg
     5161 agctgtgccg acagttctac gacctcggct gggtcagcgg caccggcggt gggatcagca
     5221 tccgcggcgc ggagggcatc tacatcgcgc cctccggcgt gcaaaaggag cgcatgcgac
     5281 ccgaggacat ctaccttctg gacgagaagg acctcgacga cgccaaggtc gtgtgcgccc
     5341 cggccgacgg ctcgctcaag atcagcgagt gtcggccgct gttctacaac gcctatcgcc
     5401 tgcgcaacgc cggggcggtg ctgcacagcc actcgatatg gtcggtgctc gcctcgcgga
     5461 tgttcagcaa cgagttcgtg tgtcagggcc tcgagatgca aaagggtctg cgcggggtcg
     5521 gctgcttcga cacgctgcgc gtcccgatca tcgccaacac ggcgcgagag gccgagctga
     5581 cggcgagcat ggcggcggcg atcgagggct acccgcaggt cgacgcggtg ctggtcgccg
     5641 ggcacggcgt gtacgtgtgg ggcgcgacct gggcccaggc caagacgcag gccgagtgct
     5701 acgatttcct gttccgcgcg gtggtcgagg cgcatcgcct cggcattcgc ctgactacca
     5761 ctcctgcctg aggcccgccg accatgcacc tgtcctggct tgaatccgac gcgagcacct
     5821 tgagctccgc accgatcgac accgcggatc tcaacgcagc gggcgtgttg tacgagcgcc
     5881 tcgggctcga ggactatcag gcctcgctcg agcggctcaa ggcggagcgc gggtacgtga
     5941 cgcaggacat cgtcgagctg cacccggaga tgccgaatct ccaggcggtg tgcgacaagt
     6001 tcaaggacga gcacctgcac cgcgacgacg aggtccgctt cgtgctcgcg ggcgagggca
     6061 tcttcgacat ccgcgcgaag gatggtcgat ggatgcgcgt gcaggtcgag ccgggcgacc
     6121 tgatcgtcgt gccggcggac ctctaccacc gattcttttt gaccgagcgg cagcagatcc
     6181 gctgcgtgcg cttatttaaa gacgccgccg gctgggtccc cgagtaccgg gtcgcctgaa
     6241 tttcaccttt gatcagtcct gatcatcacc aacgagaggc agtttaagag atgtccgcaa
     6301 gcaccgtgac gaagatccac cccgagcaga cccaagactt caaggtcgcc gacatgtccc
     6361 tggccgagtg gggccgcaag gagatcgagc tggccgagaa ggagatgccg ggcctgatgt
     6421 cgctgcgctc caagtacggc gccgagaagc cgctcaaggg cgccaagatc g
//
LOCUS       DASWNJ010000590         2195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_469950, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000590 DASWNJ010000000
VERSION     DASWNJ010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469950"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1512
                     /locus_tag="VGB85_09910"
     CDS             <1..1512
                     /locus_tag="VGB85_09910"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0134386.1"
                     /translation="KPIVHPEPIPLDEETDFGDLGSDDDETDFSELMHTRRPNGEPVP
                     TPAPRQSAHDSGKSVLPQPPLKADSGRSPAPQPAASKPDSGRSPLPQPAATSKPDTGS
                     SPAPQDDAARPSQPRPDTGKSPVTQPAPSLRPILGGPAPTAPNPAPVLGAPVLGAPVL
                     GGPRPTPTLTPVPTPAPVTLAEPEPEPEPDALAEPDPIAEPDALAEPDPLDDLGSLDD
                     DVDLGLGDDLDDLELDADAVLDDDLLGDGPLDDDALGDDPLGDADDGLAAGGLDDTDH
                     EDEPEPLGDSSLTDASMSDRSVAIDLTDMSGVSALSGLNEGNEGDEALGDLDEDLELE
                     DLDLDAAEEIVEDGEGFGEDEGDHDGDHGYRRSDEDTDGGYKQHAVETAGALAATRYA
                     QAEEAMREGEFDQAVALFEDAYANGHDVADLHAHLAFCRFRASGNDPSTAAGALELLD
                     WAEQADPGLPMIHAYRGAILVGLGRQEEADECFDRALYLDPENELAIAYTRGE"
     gene            complement(1532..>2195)
                     /locus_tag="VGB85_09915"
     CDS             complement(1532..>2195)
                     /locus_tag="VGB85_09915"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HEY0134387.1"
                     /translation="DAGGGSWDSDSMPPPMSPSKGSPDPMGFTSCDGTNDCSNTIEDQ
                     WDLGGGDAEDKMWFSFELKAPSLANGDIADANGYTFDFAYFSAEFPEYVDSPFNDIFV
                     VWQDSEEYTGNVTFINGQPLTVTALWPVDYQGECDFFDPNCQGDDPHLAGTGHSEDGG
                     ATGWYKATGGVTPGETFTLAFAIFDMGDSDYDTTAILDNWQWDCEGCVPNEVNSCGIE
                     PQ"
BASE COUNT          325 a          860 c          737 g          273 t
ORIGIN      
        1 aagccgatcg tccatcccga gccgatcccg ctcgacgagg agaccgactt cggcgacctc
       61 ggcagcgacg acgacgagac cgacttcagc gagctgatgc acacgcgccg gcccaacggc
      121 gagccggtcc cgaccccggc gccgcgccag agcgcccacg actcgggcaa gagcgtgctg
      181 ccgcagccgc cgctcaaggc cgactccggg cgctcgcccg cgccgcagcc cgccgcgagc
      241 aagcccgact ccggtcgctc gcccctgccc cagcccgccg cgacgagcaa gcccgacacc
      301 ggcagctcgc ctgccccgca ggacgacgcg gctcgcccgt cgcagccgcg gcccgacacc
      361 ggcaagagcc cggtgaccca gccggccccg tcgctgcgac cgatcctcgg cggaccggcc
      421 ccgaccgcgc cgaatcccgc gccggtcctc ggcgccccgg tcctcggcgc gcccgtcctc
      481 ggcggcccgc gtccgacccc gacgctcacg cccgtgccga cccccgcccc cgtcacgctc
      541 gccgagcccg agcccgagcc cgagcccgac gccctcgccg agcctgaccc gatcgccgag
      601 cccgacgcgc tcgccgagcc cgacccgctc gatgacctcg gcagcctcga cgacgacgtc
      661 gacctcggcc tcggcgacga cctcgacgac ctcgagctcg acgccgacgc ggtgctggac
      721 gacgacctgc tcggcgacgg cccgctcgac gacgacgctc tcggcgacga cccgctcggc
      781 gacgcggacg acggcctcgc ggccggcggc ctcgacgaca ccgatcacga ggacgagccc
      841 gagccgctcg gcgactcctc gctgaccgac gcctcgatga gcgaccgcag cgtggcgatc
      901 gacctcaccg atatgtcggg ggtcagcgcc ctgtcgggcc tcaacgaggg caacgagggc
      961 gacgaggcgc tcggcgacct cgacgaggac ctcgagctcg aggacctcga cctcgacgcc
     1021 gccgaggaga tcgtcgagga cggcgagggc ttcggcgagg acgagggcga ccacgacggc
     1081 gatcacggct atcgccgcag cgacgaggac accgacggcg gctacaagca gcatgcggtc
     1141 gagaccgccg gggcgctcgc ggcgacccgc tacgcgcagg ccgaggaggc gatgcgcgag
     1201 ggggagttcg accaggcggt cgcgctgttc gaggacgcct acgccaacgg ccacgacgtc
     1261 gccgacctgc acgcgcacct cgcgttttgc cgcttccggg ccagcggcaa cgacccctcg
     1321 accgccgcgg gcgcgctcga gctgctcgac tgggccgagc aggccgaccc cggcctgccg
     1381 atgatccacg cctatcgcgg cgcgatcctc gtcggcctcg gacgtcagga ggaggccgac
     1441 gagtgcttcg accgcgcgct ctacctcgac cccgagaacg agctggcgat cgcctacacc
     1501 cgcggcgagt gacgccgcgg gtgcccgtgc ctcactgcgg ctcgatgccg cagctgttga
     1561 cctcgttggg cacgcagccc tcgcagtccc actgccagtt gtcgaggatc gccgtggtgt
     1621 cgtagtccga gtcgcccatg tcgaagatcg caaacgcgag cgtaaaggtc tcgcccgggg
     1681 tcacgccgcc ggtcgccttg taccagccgg tcgcgccgcc gtcctcgctg tggccggtgc
     1741 ccgcgaggtg cgggtcgtcg ccctggcagt tcgggtcgaa gaagtcgcac tcaccctggt
     1801 agtcgaccgg ccacagcgcc gtgaccgtca gcggctggcc gttgatgaag gtgacgttgc
     1861 ccgtgtactc ctccgagtcc tgccacacga cgaagatgtc gttgaacggg ctgtcgacgt
     1921 actccgggaa ctcggccgag aagtacgcga agtcgaaggt gtagccgttg gcgtcggcga
     1981 tgtcgccgtt ggccagcgac ggcgccttga gctcgaagct aaaccacatc ttgtcctcgg
     2041 cgtccccgcc cccgaggtcc cactgatcct cgatcgtgtt cgagcagtcg ttggtgccgt
     2101 cgcagctggt gaagcccatc gggtccgggc tgcccttgct cgggctcatc ggcgggggca
     2161 tcgagtcgct gtcccagctg ccgccgccgg cgtcg
//
LOCUS       DASWNJ010000591         2531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_435630, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000591 DASWNJ010000000
VERSION     DASWNJ010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435630"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..>2531
                     /locus_tag="VGB85_09920"
     CDS             284..>2531
                     /locus_tag="VGB85_09920"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEY0134388.1"
                     /translation="MQTSLLSLVPPARDLQEVHTSPVRGSHSLWADDEPPADAPSIWD
                     DEPFAPASNTTYDYISEDRPRLADAPSRGSSEPISNPAPAPPSKARRASVSAGDLTIP
                     TSKARRARKSEDGPAPPGKASRAPGDEPVSKPRRARASAVEAVPSKAPRARTSGSELA
                     TSPSTAPRAHVSADDPPPSKPRRARASEPPAAASDAPRTLGSADEPPSKPRRVRASPD
                     DAPLAAPTRFHRTAADDLRTLLRSRFNFPQFRPYQEAVCRAATEGRDLLLVMPTGAGK
                     SLCYQLPGVARGGTTLVISPLIALMEDQSTRLSAQGFRSERIHSGRGRAESRQACADY
                     LAGKLDFLFIAPERLRVPGFPEMLARRLPALIAVDEAHCISQWGHDFRPDYRMLGQRL
                     PALRPAPIVALTATATPTVQDDIVAQLGLKGAARFIHGFRRTNIAIEVLERNPGERAA
                     TIAELLSDRARRPAIVYAATRKSAEDLAGTLGPRLAAAYHAGMSSEERDRVQTAFLAG
                     QREVIVATTAFGMGIDKADVRTIIHAALPGSLEGYYQEIGRAGRDGKPSTAILMHAYV
                     DRKTHEFFHERDYPEPGQLAAIFSRLPKTPAARDTLNKRRGRAAREAFEKALEKLVIH
                     GGALEAGDGEISKGSASWQAGYAAQREHKQTQLEVMARFAGSQGCRMVHLIRHFGDQE
                     DGGEVCGICDVCAPDRCIAREFRAPSASEQTHAARILAALADGDGLATGRLHLETFPK
                     GDVDRNSFE"
BASE COUNT          416 a          969 c          804 g          342 t
ORIGIN      
        1 ccgcgacggc aagtcgctcg cggcgaccga caagggcatt cacctgatct cggtggtgca
       61 cccggacgtc aagagtccgt cgatgaccgg cgagtgggag gccaagctca aacgcatggc
      121 ccgcggcgag ggcgagctgt cgaccttcat ggccggcatc gaggcgttcg tcagcggcgt
      181 gatcggccgg gtcacgaacg ccagcaattc aaacgagccc cctcccgacg gagcacgcgc
      241 tgcgcccccg cagacgccgc gcccgcgggc cgcgaacgcg ccgatgcaga cgagcctgct
      301 ctcgctcgtg ccccccgcgc gggacctcca ggaagtccat acatcgcccg tgcgtggatc
      361 tcactcgctg tgggcggacg acgagccccc ggctgacgcc ccgagcattt gggacgacga
      421 gcctttcgcg cccgcgagca acaccaccta tgattatatt tcggaggacc ggccccgtct
      481 cgcagacgcg ccctcccgcg gatcgtccga acccatatca aatccggcgc ccgccccacc
      541 gagcaaggcc cgtcgcgcct ccgtatctgc cggcgatctc accataccga cgagtaaggc
      601 ccgcagagcc cgcaaatccg aggacgggcc ggccccaccg ggcaaagctt cgcgcgcccc
      661 cggggatgag ccggtcagca agccccgccg cgcccgcgca tccgccgtcg aagctgtgcc
      721 gagcaaagct ccccgcgccc gcacatccgg gagcgagctt gcgacctcgc ccagcacagc
      781 cccccgcgct cacgtctctg cagacgaccc accgccgagc aaaccccgcc gcgcccgcgc
      841 gtccgaacct cctgccgcag cgagcgacgc tccccgaacc ctcggatccg cggacgaacc
      901 tccgagcaaa ccccgccgcg tccgcgcatc cccggacgac gccccgctcg ccgcccccac
      961 caggtttcat cgcaccgccg cggacgacct gcgcacgctg ctgcggtccc gcttcaattt
     1021 tccacagttt cgcccgtacc aggaggcagt gtgtcgcgcc gcgaccgagg ggcgcgacct
     1081 gttgctggtc atgccgaccg gcgccggcaa atcgctatgt tatcagttgc ccggcgtcgc
     1141 gcgcggcggc accacgctcg tcatcagccc gctgatcgcc ctgatggaag atcagtcgac
     1201 gcggctctcg gcccagggct ttcgctccga gcgcattcac tccggccgcg ggcgcgccga
     1261 gtcgcgccag gcctgcgccg actatctcgc gggcaagctc gacttcctgt tcatcgcccc
     1321 cgagcgcctg cgcgtgcccg gcttcccgga gatgctcgcc cgccgcttac ccgcgctgat
     1381 cgccgtcgac gaggcgcact gcatctcgca gtggggccac gactttcgcc cggactatcg
     1441 catgctcggc cagcggctgc ccgcgctgcg cccggccccg atcgtcgcgc tcaccgcgac
     1501 cgcgacaccg acggtccagg acgacatcgt cgcgcagctg ggcctcaaag gcgccgcgcg
     1561 cttcatccac gggtttcggc gcaccaacat tgcgatcgag gtgctcgagc gcaacccggg
     1621 cgagcgcgcc gcgacgatcg cggagctgct gtcggatcgc gcgcgccggc ccgcgatcgt
     1681 atatgcggcg actcgcaaga gcgccgagga cctcgccggc acgctgggtc cgcgcctcgc
     1741 cgcggcctac cacgccggca tgagcagcga ggagcgcgac cgggtccaga ccgccttcct
     1801 cgccggccag cgcgaggtca tcgtggcgac gaccgcgttc ggcatgggca tcgacaaggc
     1861 cgacgtgcgc acgatcatcc acgcggcgct gccgggcagc ctcgagggtt attatcagga
     1921 gatcggccgc gccggccgcg acggcaagcc ctcgacggcg atcctgatgc acgcatacgt
     1981 cgatcgcaag acccacgaat tcttccacga gcgcgactac cccgagcccg gccagctcgc
     2041 cgcgattttt tcaaggttgc ccaagacacc cgccgcccgc gacactctca ataaaagacg
     2101 tgggcgcgcg gcgcgggagg cctttgaaaa ggccctcgaa aagctcgtca ttcatggcgg
     2161 cgcgctcgag gccggcgacg gcgagatctc aaagggctcg gcgagctggc aggccggtta
     2221 cgccgcccag cgcgagcaca agcagacgca gctggaggtc atggcgcgat tcgccggatc
     2281 ccagggctgc cggatggtgc atttgataag acacttcggg gatcaggagg atggcggcga
     2341 ggtctgtggg atctgcgacg tgtgcgcccc ggatcgctgt atcgcccgcg agtttcgggc
     2401 cccgagcgcg agcgagcaga cccacgcggc gcggatcctc gcggccctgg ccgacggcga
     2461 cggcctggcg acgggccgcc tccacctcga gacatttcca aaaggagacg tcgaccgcaa
     2521 ttcattcgaa c
//
LOCUS       DASWNJ010000592         4232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_576233, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000592 DASWNJ010000000
VERSION     DASWNJ010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576233"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1930
                     /locus_tag="VGB85_09925"
     CDS             <1..1930
                     /locus_tag="VGB85_09925"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HEY0134389.1"
                     /translation="CGANVADSAIDVYWRADSPNPDQAEANSAVTLADARSSAVLKLP
                     QNAVVSHAFLYWAAALDAPGTDGTVTFDRPGSFSTPVDGSANCVSIMNDASNGYQCSA
                     DVTATIQTYGAGSYRVGGVDIAKLGGVNNEGHFAGWWLVVLYQDPGQPLRNLAVFDGF
                     ESVSQGMSLDILLDGFLVPDGGIEGKLGVVAFEGDAALTGDQFFFGGGPALGNAQNPV
                     NNFFNSTRTRFGSALSVVGDLPQLTGAHNSMSGIDLDIVDITTKLTAGQTQATVTAST
                     NGDGFVLSGFITSITDLRPDFTTSVKTAVDVNGGALVVGDEVEYTIEVKNTGSDAAID
                     LVLTDPLPAGVTYAPGSLEITAGANAGAKTDGPGDDQAEYDMNAHTLVVRLGMDADDT
                     AGGTLDIGANTAVKFRVTVDAAGQIDNQAIVDAAGMLGAGSTKTPTGDGNFPGAPTSL
                     LAESCETDAQCMDPKLSHCDAASEPSACVECLDDGHCTAGWTCDAAAHTCACTPAEAE
                     LCDDGLDNDCDSEIDEDCEPDTTSGDPSTSGDPSTSSDPSTSGDTPTGGGTSGGDSIS
                     GGDTSTSGDTSTSGGGEVPTGGPDDGSSGNSGTSDATGTDPGIDGEGGCGCDTRGPDA
                     GGLLLLAVAGFAARRRRR"
     gene            2546..3781
                     /locus_tag="VGB85_09930"
     CDS             2546..3781
                     /locus_tag="VGB85_09930"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134390.1"
                     /translation="MQLFHKLRGELGEFLVGQDHGVLLVHAPLDQIPLLYKILAELEQ
                     RSPDVHLLFPHAFTSACAYAELVGECIAASIREVHGPDAALPWPADEVTTPRPTLPPF
                     GPCAAGSPARQIRAALCAARDLLPRRGTPVRLVAGLCPLSADAGPYRTLARELLAQEA
                     PVAPWFHGMRVLLYSPPGEPLLPHPRRFVRVLHLDASPAALQESLRQEAHDPRQPARR
                     RAQALLQLAALDHGHRRLAEARARYLEVLAHAQDTDDPLLAALAMNGLGDVEAREGRP
                     VEARAWYERALVPAGRTRAAMVLLLVARNLGHLHFEARRLADAEAYFDAVQQLATRTH
                     DSETRLLALEWRGLCQPDRPADRAESFTAAAVLARDLARSGAASDHHDRLLGRLRDCP
                     LHRCPAELRARIQHELTIA"
     gene            3794..>4232
                     /locus_tag="VGB85_09935"
     CDS             3794..>4232
                     /locus_tag="VGB85_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HEY0134391.1"
                     /translation="MPQTAAPAHTPPEVPAAPLDVLQAGIDSALGVVELITEAPMDAV
                     NLAVGRATDAISQALPSFPAATMGMMAVALPHAHGHPPSLIPPAPPVPLPVIGTITLG
                     CCVSVLINGVPAARAGDLGFSPTCCGFVPMFEVYTGSSKVFIGG"
BASE COUNT          623 a         1679 c         1362 g          568 t
ORIGIN      
        1 gtgtggcgcc aacgttgccg acagcgcgat cgacgtgtac tggcgcgccg attcgccgaa
       61 ccccgaccag gccgaggcca acagcgcggt cacgctggcg gacgctcgca gctccgcggt
      121 cctcaagttg ccgcagaacg ccgtcgtgtc ccacgcattc ctctactggg ccgcggccct
      181 cgacgcgccc gggaccgacg gcaccgtcac cttcgaccgc ccgggcagct tctccacccc
      241 ggtcgacggc tcggcgaact gcgtgtcgat catgaacgac gcctccaacg gctaccagtg
      301 cagcgccgac gtcacggcga cgatccagac gtacggcgcc gggtcctacc gcgtcggcgg
      361 ggtcgacatc gcgaagctcg gcggcgtcaa caacgagggg cacttcgccg gctggtggct
      421 ggtcgtcctc taccaggacc cgggccagcc gctgcgtaac ctcgcggtat tcgacgggtt
      481 cgagagcgtg tcacagggca tgtcgctcga catcctcctc gacgggttcc tggtccccga
      541 cggcggcatc gagggcaagc tgggcgtcgt cgcgttcgag ggcgacgccg ccctcacggg
      601 cgaccaattt ttcttcggcg gcggcccggc cctcggcaac gcgcaaaacc cggtcaacaa
      661 cttcttcaac agcacgcgca cgcgcttcgg cagcgcgctg tcggtcgtcg gcgacctccc
      721 gcagctgacg ggcgcccaca actcgatgtc cggcatcgac ctcgacatcg tcgacatcac
      781 caccaagctc acggccgggc agactcaggc gacggtgacc gcgagcacca acggcgacgg
      841 atttgttctg tccggcttca tcacctcgat caccgacctt cggcccgact tcacgacctc
      901 ggtcaagacg gccgtcgacg tgaacggcgg cgcgctcgtg gtcggcgacg aggtcgagta
      961 cacgatcgag gtcaagaaca ccggcagcga cgccgcgatc gacctcgtgc tcaccgaccc
     1021 gttgccggcc ggggtgacct acgcgccggg ttcgctcgag atcaccgcgg gcgcgaacgc
     1081 cggcgccaag accgacggcc cgggcgacga ccaggccgag tacgacatga acgcgcacac
     1141 gctcgtcgtc cgcctcggca tggacgccga cgacaccgct ggcgggacgc tcgacatcgg
     1201 cgcgaacacg gcggtgaagt tccgcgtgac cgtcgacgcc gccgggcaga tcgacaacca
     1261 ggcgatcgtc gacgccgccg gcatgctcgg cgccgggtcg accaagaccc cgaccggcga
     1321 cggcaacttc ccgggcgcgc cgacgtccct gctcgccgaa tcctgcgaga ccgacgcgca
     1381 gtgcatggac cccaagctgt cgcactgcga cgccgcgagc gagccgagcg cctgcgtcga
     1441 gtgcctcgac gacggccact gcaccgccgg ctggacctgc gacgccgcgg cgcacacctg
     1501 cgcgtgcacg cccgccgagg ccgagctgtg cgacgacggc ctcgacaacg actgcgacag
     1561 cgagatcgac gaggactgcg agcccgacac gacgagcggc gacccgtcga cgagcggcga
     1621 cccgtcgacg agcagcgacc cgtcgacgag cggcgacacg ccgaccggcg gagggacctc
     1681 cggcggcgac tcgatctccg gcggcgacac gtcgaccagc ggcgacacgt cgaccagcgg
     1741 cggcggtgaa gtcccgaccg gcggccctga cgacggttcg agcggtaact ctggcacctc
     1801 cgacgcgacc ggcacggacc cggggatcga cggcgaaggc ggctgcggct gcgatacccg
     1861 cggccccgac gcgggcggcc tgctgctgct cgccgtcgcc ggattcgcgg cgcggcgccg
     1921 tcgccgttga gcagaccgcg ggcgcagctc agcggtcgag cggttcgatc tcgatgcgcg
     1981 acaccccgat gcgcagctgg tcgtgaccac caaccccagt ggcccgccaa cctgctacac
     2041 ccgttccgcg cggcatccac aagaccaagc acgcttgcac cggcaacgcg caatcgccaa
     2101 atctgcttgc atggtgaccg cttgcgcatg caagcgcagt ggcatggcat ccagcctcac
     2161 ccgatcccca gtacggttgg ggcttgcacg aacgccgcga tggcggcaga tcgagagcgt
     2221 agctgtcttt tgaagcgagg tgtgacgagt catggcgatc gcccttatca agcgtgacgt
     2281 gccacatccc gagatcgccg gcgcggcgac gcgcgtggga ggtcgccgcc gtcgagacgc
     2341 cgctgccgcg caagccgact ccaggcccat tgcgaccggc cgacggaccg gaatgcggcg
     2401 atcgctcgcg tcgagcgtcg gcatcggcgc cccggacagc gtcgacctcg acggccgcgc
     2461 cttgctcacc gcgaccgcga agacagcacc gcaatcggca tgcacgagct ctcgggtgcg
     2521 ctgatcaaga tcgactgagg cccgcatgca gctgtttcac aagctccgcg gcgagctcgg
     2581 cgaattcctc gtcggccagg atcacggcgt gctgctcgtg cacgcgccgc tcgaccagat
     2641 cccgctcctc tacaagatcc tcgccgagct cgagcagcgc tcgcccgacg tccacctgct
     2701 gttcccccac gccttcacct ccgcctgcgc ctacgcggag ctcgtcggcg agtgcatcgc
     2761 cgccagcatc cgcgaggtcc acggccccga cgccgcgctg ccatggcccg ccgacgaggt
     2821 gacgaccccg cgtcccacgc tcccgccctt cggtccctgc gctgcgggtt cgccggctcg
     2881 ccagatccgc gcggcgctgt gcgccgcgcg cgacctcctg cctcgccgcg gcacccctgt
     2941 gcgcctcgtc gccgggctct gcccgctgtc cgccgacgcc ggaccctacc gtaccctcgc
     3001 ccgcgagctg ctcgcccaag aggcccccgt cgccccctgg ttccacggca tgcgcgtgct
     3061 gctctacagc cccccgggcg agccgctcct gccccacccg cgtcgcttcg ttcgcgtcct
     3121 ccacctcgac gcctcgcccg ccgcgctgca agagagcctg cgccaggagg cccacgaccc
     3181 gcgtcagccg gcccgtcgac gcgcccaggc gctcctgcaa cttgccgccc tcgaccacgg
     3241 ccaccgccgg ctcgccgagg cccgcgcccg ttacctcgag gtcctcgccc acgcccagga
     3301 cacggacgac ccgctgctcg ccgccctcgc gatgaacggc cttggagacg tcgaggcccg
     3361 tgagggccgc ccggtcgagg cccgggcctg gtacgagcgc gccctcgtcc ccgccgggcg
     3421 tacgcgcgcc gcgatggtcc tgctgctggt cgcgcgcaac ctcggtcatc tgcacttcga
     3481 ggcgcgtcgc ctcgctgacg ccgaggccta cttcgacgcc gtccagcagc tggcgacccg
     3541 cacccacgac tccgagaccc ggctgctcgc gctcgagtgg cgcggcctgt gtcagcccga
     3601 ccgcccggcc gaccgcgccg agagcttcac ggcggcggcc gtgctcgccc gcgacctcgc
     3661 ccgcagcggg gccgccagcg atcaccacga tcgcctgctc ggtcgcctgc gcgactgccc
     3721 cctgcaccgc tgccccgccg agctgcgcgc ccgcatccag cacgaactga cgatcgcctg
     3781 accgaggtct gccatgcccc aaaccgccgc tcccgcgcat accccccccg aggtcccggc
     3841 cgctccgctc gatgtcttgc aagccggcat cgactccgct ctcggcgtgg tcgagctgat
     3901 caccgaggcg ccgatggacg cggtcaacct cgccgtcggc agggccaccg acgccatctc
     3961 gcaggcgctg ccctcgttcc cggcggcgac gatggggatg atggccgtgg cgctcccgca
     4021 cgcgcacggg catccgccga gcctgatccc gcccgcgccg ccggtccccc tcccggtgat
     4081 cggcaccatc acgctcggct gctgtgtctc cgtgctgatc aacggcgtac cggcggctcg
     4141 cgccggcgac ctcggcttca gcccgacctg ctgcggcttc gtgccgatgt tcgaggtcta
     4201 caccggcagc tccaaggtgt tcatcggcgg gg
//
LOCUS       DASWNJ010000593         3313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_541214, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000593 DASWNJ010000000
VERSION     DASWNJ010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541214"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2197)
                     /locus_tag="VGB85_09940"
     CDS             complement(<1..2197)
                     /locus_tag="VGB85_09940"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEY0134392.1"
                     /translation="MIVTHPLSTLSRTDLAVVGGKAANLGELTRLGLDVPDGFVVTTA
                     AYAEQMRVWGLAERLAPLLAVGDWEATAAEASALCQRGELLPEIDRAVREAHRALAAP
                     AVAVRSSATAEDLADASFAGQHETFLDVAGEAAVLLAIRGCWASLWSPRALGYRAARG
                     VDHLGIDIAVVVQRMVPAEFAGVLFTVDPVAQRADRMLLEVAPGLGEAVVSGHTTGDV
                     YRLRRPPFVSAASPEPADVAIEDRDRRDPERPAPNDTLVLELGRLALAIEAHFGTPQD
                     VEFALVGGRIHILQSRPITTLVDAEIEPLPELPELRGLQRRMAEANDAGRFPVAPRPL
                     DQWGFRVVIPALAQTLRFIGMAVDPADERATVDVLWRERMIPPKARPTLRLLRLPGKL
                     AAALRRDWLAWWERDIFPEITEVCAPVDLRALDDAALLRHADRIATTYTEVLRRRFEG
                     IIGQMAALLLRVPVRLAVGKQRAPAVLGDLVSGLHTRTSEVNAALYQLARRAASAGPE
                     VTTAIREDRLDALGDSQAGRTFLAEVDAFLAAHGHRETTGFYLSAPTWQTDPAPMWGL
                     LRGLLDASEPPSEAAGVRRYQAALAEVTGKLRRVPGLARAFTRLVEALRRSTVYRERS
                     HYDLTRFFSALQAVIGEAARRLHERGDLPAPADVFYLTEGELRTWLEGQVPARAEVER
                     LLRRRRATYGVVNGRWQKWAFRPGAADGSANLLRGIGASSGVVRGKARII"
     gene            complement(2260..>3313)
                     /locus_tag="VGB85_09945"
     CDS             complement(2260..>3313)
                     /locus_tag="VGB85_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134393.1"
                     /translation="LLHTLNTSPRDATSGLWLTLELQHPRAVSAVVSPMSLQYRALDI
                     PGGQESRFTGVCDLQAAHGFVSPEPLDLRVHYILPHYHYLGNYFDVTVIGGERDGESI
                     YNLTGFNGEGNGKTFDPPLALPGATGLRFTCGYDNWRNQSVQWGNGDGEMCVLLALVE
                     SDAVMGATVDALNHLVGAREGIHYYEGLCLAGAVAKQPSQGPPSADERAGPLYVPPVD
                     PADADLPPVPACVDVDPAIAADEPATLRSIRDTIFAPSCTFSACHGEAAVAGLDLQAD
                     DLRARLLAHAVAGTPLITPGDPERSWLYQVLSRCEPTTPDGLGARHMPLNAPFLLAPA
                     LVNKVRAWIVAGAPDD"
BASE COUNT          452 a         1120 c         1330 g          411 t
ORIGIN      
        1 ggatgatgcg cgccttgccg cggacgaccc cggaggacgc gccgattccc cggagcaggt
       61 tcgccgatcc gtccgcggcg ccggggcgaa acgcccactt ttgccagcgg ccgttgacga
      121 cgccgtaggt cgcgcgccgg cgacggagca ggcgttcgac ctctgcgcgc gccggtacct
      181 gtccttcgag ccaggtccgc agctcgccct cggtcaggta gaacacgtcc gcgggcgccg
      241 gcagatcgcc gcgctcgtgc aggcggcgcg cggcctcgcc gatcaccgcc tgcagcgcgg
      301 agaagaagcg ggtcagatcg tagtgcgagc gctcgcggta cacggtcgac cggcgcagcg
      361 cctcgacgag ccgcgtgaac gcgcgggcga ggcccgggac gcggcgcagc ttgcccgtga
      421 cctcggcgag cgcggcctgg tagcggcgca cgccggcggc ctccgagggc ggctcggagg
      481 cgtcgagcag gccgcgcagc aggccccaca tcggcgcggg gtcggtctgc caggtcggcg
      541 ccgacaggta gaagccggtg gtctcgcgat ggccgtgcgc ggcgaggaag gcgtcgacct
      601 cggcgaggaa ggtccggccg gcctgcgagt cgcccagcgc gtcgagccga tcctcgcgga
      661 tcgccgtcgt gacctcgggc ccggcgctcg cggcccggcg cgcgagctgg tacagcgccg
      721 cgttgacctc cgaggtgcgg gtgtgcaggc cggacacgag gtcgccgagc acggcagggg
      781 cgcgttgctt gccgaccgcg aggcggaccg ggacgcgtag caagagcgcg gccatctggc
      841 cgatgatccc ctcgaagcgg cggcgcagga cctccgtgta ggtggtggcg atccggtcgg
      901 cgtggcgcag cagcgcggcg tcgtcgagcg cgcgcaggtc gaccggcgcg cagacctccg
      961 tgatctcggg aaaaatgtcg cgctcccacc acgccagcca gtcgcggcgc agggcggccg
     1021 cgagcttgcc cggcagccgc agcaggcgca gggtcggacg cgccttcggc gggatcatgc
     1081 gctcgcgcca cagcacgtcg accgtcgcgc gctcgtcggc cgggtcgacg gccatgccga
     1141 tgaagcgcag cgtctgggcg agcgccggga tcacgacgcg aaagccccac tggtcgagcg
     1201 gccgcggggc gaccgggaag cggccggcgt cgttggcctc ggccatgcgc cgctgcagcc
     1261 cgcgcagctc cgggagctcc ggcagcggct cgatctccgc gtcgacgagc gtggtgatcg
     1321 ggcgcgactg caggatgtgg atccggccgc cgacgagcgc gaactcgacg tcctgcgggg
     1381 tcccgaagtg cgcctcgatc gcgagggcga ggcggccgag ctcgagcacg agggtgtcgt
     1441 tcggcgccgg acgctcgggg tcgcgacgat cacggtcctc gatcgcgacg tcggccggct
     1501 ctgggctcgc ggcgctgacg aagggcgggc ggcgcaggcg gtacacgtcg ccggtcgtgt
     1561 gcccggagac gacggcctcg ccgaggcccg gcgcgacctc gagcagcatg cggtcggccc
     1621 gttgcgcgac cgggtcgacg gtgaacagca cgccggcgaa ctcggcgggg accatgcgtt
     1681 gcacgacgac ggcgatgtcg atcccgaggt ggtcgacgcc ccgcgcggcg cgatagccga
     1741 gcgcgcgcgg gctccacagg gacgcccagc acccgcggat cgccagcagc accgcggcct
     1801 cgcccgcgac gtcgaggaag gtctcgtgct ggccggcgaa cgaggcgtcg gcgaggtcct
     1861 cggcggtcgc cgacgagcgg accgcgacgg ccggggcggc gagcgcacga tgggcctcac
     1921 gcacggcgcg gtcgatctcc gggagcagct cgccgcgctg gcacagcgcg ctggcctcgg
     1981 cggcggtggc ctcccagtcg ccgaccgcga gcagcggcgc gaggcgctcg gcgaggcccc
     2041 acacgcgcat ctgttcggcg taggccgcgg tggtgacgac gaagccatcc ggtacatcga
     2101 ggccgaggcg ggtcagctcg ccgaggttgg ccgccttgcc gccgacgacg gcgaggtcgg
     2161 tgcgggacag tgtgctcaat ggatgggtga cgatcatcgc ggcctcctcg gtcgactgtg
     2221 cgccggtgcg tgcggggttt gcaagcgagg gcgtggcggt caatcgtcgg gcgcgccggc
     2281 gacgatccac gcgcggacct tgttgacgag cgccggcgcg agcaaaaacg gggcgttgag
     2341 cggcatgtgg cgcgcgccga ggccgtccgg cgtcgtcggc tcgcagcgcg acagcacctg
     2401 atacagccag ctgcgctcgg ggtcgccggg ggtgatcagc ggggtgccgg cgacggcgtg
     2461 cgcgagcagc cgggcgcgca ggtcgtcggc ctggaggtcg aggccggcga ccgcggcctc
     2521 gccgtggcag gccgagaagg tgcacgaggg ggcgaagatc gtgtcgcgga tgctccgcag
     2581 ggtcgcgggc tcgtcggccg cgatcgccgg gtcgacgtcg acgcaggccg gcaccggcgg
     2641 caggtcggcg tcggcggggt cgacgggcgg gacgtacagc gggcccgcgc gctcgtccgc
     2701 gctcggcggg ccctggctcg gctgcttggc gaccgcgccg gcgaggcaca ggccctcgta
     2761 ataatgaatg ccctcgcgcg cgccgacgag gtgattgagg gcgtcgacgg tcgcgcccat
     2821 caccgcgtcg gactcgacga gcgcgagcag gacgcacatc tcgccgtcgc cgttgcccca
     2881 ctgcaccgat tgattacgcc agttgtcgta gccgcaggtg aagcgcagcc cggtcgcgcc
     2941 gggcagcgcc agcggcgggt cgaaggtctt gccgttgccc tcgccgttga agccggtgag
     3001 gttgtagatc gattcgccgt cacgctcgcc gccgatgacg gtgacgtcga agtagttccc
     3061 gaggtagtga taatgcggca ggatataatg gacgcgcagg tcgagtggtt cggggctgac
     3121 gaagccgtgg gcggcctgga ggtcgcacac gccggtgaag cgcgactcct gcccgcccgg
     3181 gatgtcgagc gcccggtatt gcagggacat cggcgagacg accgcggaga cggcccgcgg
     3241 gtgctgcaat tcgagggtca gccacaggcc ggaggtggcg tcgcgcgggg aggtgttgag
     3301 ggtgtgcagc agg
//
LOCUS       DASWNJ010000594         2229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_576260, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000594 DASWNJ010000000
VERSION     DASWNJ010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576260"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VGB85_09950"
     CDS             <1..717
                     /locus_tag="VGB85_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134394.1"
                     /translation="DLLGKAIEVGRIYDPVVPLLARALQETGDRTLLDLCSGGGGPVP
                     SLRRLLARDHGIDVEARLSDFYPNTAAFERIAAHEQGRVTYEATPIDATRVPRELAGF
                     RTMFTCMHHFRPAQAEAILRDAWTADRGIAVFEFTERSVAGMAQMFLSPISAMVLTPL
                     IRPFRWSRLALTYVVPVLPAVFMFDGIVSQLRTYTPDELRAMTRPLQRPGYRWEIGTV
                     KHPLLPGRVSYLLGLPTAPR"
     gene            860..1558
                     /locus_tag="VGB85_09955"
     CDS             860..1558
                     /locus_tag="VGB85_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134395.1"
                     /translation="MANFRVHVLGAAIPSLIAAGLAVAGGLATPVVGGACFGLGVLGG
                     MLPDIDADSSVPTRIIFTALAVGAAVAVALALRDGTAPGLVALASLAAFLAIRVGLRG
                     FTAWLCVHRGLVHSLPFAALVGVLVVLAASRGFHLAPTPAWLTGVFLTGGFIIHLALD
                     ELYSVDLLGARMKRSFGTALKLGSRRQLFGTAVLYASVIGLVLVAPPADRFLRDLTSS
                     RTRAAVAATFSGLR"
     gene            1635..>2229
                     /locus_tag="VGB85_09960"
     CDS             1635..>2229
                     /locus_tag="VGB85_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PNGase F N-terminal domain-containing protein"
                     /protein_id="HEY0134396.1"
                     /translation="MRLSSFSCVCISTLLACGTSGGEGEGSDTSTGTTTNEPTTSEPT
                     TGGPPTTDAATTDASTTTASTTDASTGTTGEPEPPEPQVLTVLDGVVFYDGYAATVDE
                     PVPEGVIRHNNALLAIKATDEQLAAIQTTLQIGVIVGALCDNYDRIGSVNLALVPRGA
                     TTYVPAEVQRIELARFITPFMNMNKSPKSVPYQWQADN"
BASE COUNT          291 a          853 c          737 g          348 t
ORIGIN      
        1 gacctgctcg gcaaggcgat cgaggtcggc cggatctacg atccggtcgt gccgttgctg
       61 gcgcgggccc tgcaagagac cggcgatcgg acattgctcg acctgtgctc gggcggcggc
      121 ggtccggtgc cgtcactgcg ccggctgctg gcgcgcgacc acgggatcga cgtcgaggcc
      181 cggctcagcg acttctatcc caacaccgcg gccttcgagc ggatcgccgc gcatgaacag
      241 gggcgcgtca cctacgaggc gaccccgatc gacgcgaccc gcgtcccccg cgagctcgcc
      301 ggctttcgca cgatgttcac gtgcatgcac cacttccggc ccgcccaggc cgaggcgatc
      361 ctgcgcgacg cctggaccgc cgaccgcggg atcgcggtgt tcgaattcac cgagcgcagc
      421 gtcgccggca tggcgcagat gttcctgtcg ccgatctcgg cgatggtgct gacgcccttg
      481 atccgaccgt ttcgctggtc acggctggcg ttgacatatg tggtgcccgt cctgcccgcc
      541 gtgttcatgt tcgacggcat cgtctcgcag ctgcggacat acacgcctga cgagctgcgc
      601 gccatgaccc gcccgctgca gcgccccggc taccgctggg agatcgggac cgtgaagcat
      661 ccgctgcttc cgggcagggt gtcgtacctg ctcggtctgc cgacggcgcc gcgctgacgc
      721 gagcgcatcg atcgcgcgcc gccggggcgc gccatgtcga gccggcgcct cccccgggtc
      781 gcgatagcga gcgtggaccc ggccgcgccg acccgagtta ggcgcgccgg ctgagccgcc
      841 gtaagctctg cgccgagcca tggccaactt tcgcgttcac gtcctcggtg cggccatccc
      901 ctcgctgatc gccgcgggcc tcgcggtcgc cggtgggctc gccacgcccg tggtcggcgg
      961 cgcctgcttc ggcctcggcg tgctcggcgg catgctgccc gacatcgacg ccgacagctc
     1021 cgtgccgacc cgcatcatct tcaccgcgct cgccgtcggc gcggcggtgg ccgtcgccct
     1081 cgcgctgcgc gacggcacgg cgccggggct ggtcgcgctc gcgtccctcg ccgccttcct
     1141 ggcgatccgc gtcggcctgc gcggcttcac ggcgtggttg tgtgtgcatc gcggcctcgt
     1201 ccactcgctg ccgttcgccg cgctggtcgg tgtgctcgtc gtgctcgccg cctcgcgtgg
     1261 attccacctc gcccccaccc ccgcgtggct gacgggcgtg ttcctcaccg gcggctttat
     1321 cattcacctc gcgctcgacg agctctacag cgtcgacctg ctcggcgccc gcatgaagcg
     1381 gtccttcggc accgcgctca agctcggcag ccggcgccag ctgttcggca cggccgtgct
     1441 ctacgccagc gtgatcggcc tggtgctcgt cgccccgccc gcggaccggt tcctgcgtga
     1501 cctgacctcc agccgcacgc gggccgcggt cgcggcgacc ttctccggcc tgcgctgatc
     1561 gccacccgcg aggacgtgta ccgcctcacc ccgggccgct cggcgccgcg cccgccagct
     1621 gataccctcg tctcatgagg ctctcgtcat tctcctgtgt atgtatctcg acactgctcg
     1681 cctgcgggac ctccggcggc gagggcgagg gcagcgacac ctcgaccggc acgacgacga
     1741 acgagccgac gacgagcgag ccgaccaccg gcggcccacc gaccacggac gcggccacca
     1801 ccgacgcgag caccacgacc gcgagcacca ccgacgcgag cactgggacc accggcgagc
     1861 ccgagccgcc cgagccgcag gtgctcaccg tgctcgacgg ggtcgtattt tatgatggat
     1921 acgccgcgac ggtcgacgag ccggtgcccg agggcgtgat ccgccacaac aacgcattgc
     1981 tcgcgatcaa ggcgaccgac gagcagctgg cggcgatcca gaccacgcta cagatcggcg
     2041 tgatcgtcgg ggcgctgtgc gacaactacg accggatcgg ctcggtgaac ctggccctgg
     2101 tccccagggg cgcgaccacc tacgtgcccg ccgaggtgca gcgcatcgag ctcgcgcggt
     2161 tcatcacgcc ctttatgaac atgaacaagt ccccgaagtc ggtcccgtac cagtggcagg
     2221 ccgacaacg
//
LOCUS       DASWNJ010000595         1786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_397010, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000595 DASWNJ010000000
VERSION     DASWNJ010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397010"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..386)
                     /locus_tag="VGB85_09965"
     CDS             complement(54..386)
                     /locus_tag="VGB85_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134397.1"
                     /translation="MKAETGEVGATAEPRAARLLQAGIGCLALLGALLFLPRLLEAVG
                     ADRAASVAGGLVMHGVRFAAIAASALGFWAGAVAPLRSPVRGTGRVLGLVGLALALLL
                     GFFLRGGP"
     gene            complement(408..782)
                     /locus_tag="VGB85_09970"
     CDS             complement(408..782)
                     /locus_tag="VGB85_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134398.1"
                     /translation="MDEGSEGADMRVSDGEPLVGTWPQAVVKLGVWLASLTSVAVLGC
                     FLAMSIVTYAAACNLALATGLAGLVAVVGGGIGVRSEPDGPRQIDHRAIRHGLLLMLA
                     AFVLGVGIGFAIMSVINAHLPS"
     gene            complement(917..1249)
                     /locus_tag="VGB85_09975"
     CDS             complement(917..1249)
                     /locus_tag="VGB85_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134399.1"
                     /translation="MNDRSGELAAASELRATRLLLAGIICVALMVLLLFLPRIIDAVS
                     PDGSSFAAAVQSVHGAWFAAVAACVLGFWARAAAPARSPSRGTGLVLGIVGLVLTLLL
                     GFFIRGGP"
     gene            complement(1268..1642)
                     /locus_tag="VGB85_09980"
     CDS             complement(1268..1642)
                     /locus_tag="VGB85_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134400.1"
                     /translation="MNEDTGRADVRASDEAPLSGTGPQQLVKLGVWLACLSLVAVIGC
                     FATTIVTTLYVAGGMALATGLVGLLAAVGGGVGVRSEPGGPRRLDHRAIRHGLLLVLA
                     GFVLGTATAIAMVMAIAANWHS"
BASE COUNT          313 a          695 c          573 g          205 t
ORIGIN      
        1 ccgggtacag gggggatcac gcgaccgcga tcccgcgccc tcgccgcgga tcgctacgga
       61 ccgccacgaa gaaagaaccc gagcaggagc gcgagggcca gcccgacgag ccccagcacc
      121 cgcccggtcc ctcgcacagg cgagcggagc ggtgccactg cgccggccca gaatccgagc
      181 gcgctcgctg cgatcgcggc gaaccgtact ccgtgcatga ccaggccgcc cgcgacggac
      241 gctgctcggt cggcgccgac cgcctcgagc aggcgcggca agaacagcag cgcgccgagc
      301 agggccagac atccgatgcc ggcctgcagc agccgcgccg cccgcggctc ggccgtcgcc
      361 ccgacctcgc cagtttcagc cttcatatcg gggttcctgt gtacgctcta cgagggcaag
      421 tgcgcattga tgacgctcat gatggcgaag ccgataccga cgccgaggac gaacgctgcc
      481 agcatgagca acagcccgtg gcggatcgcg cggtgatcga tctgccgcgg cccgtccggc
      541 tcgctgcgta caccgatccc gccgccgacc acggcgacga ggccggcgag gcccgtggcg
      601 agcgccaggt tacaggcggc agcatacgtc acgatcgaca tggccaggaa acagccgagc
      661 accgcgacgc tcgtcaggct agcgagccac acgccgagct tcacgaccgc ctgcggccac
      721 gtccccacga gcggctcccc gtcactcacg cgcatatccg cgccctcgct gccctcgtcc
      781 atcgcgcgac cctaccacgg agaggctctc agctcggcat gacggacgct cggacgacga
      841 gatcgccgcg accgtacggg cgttgcaaca ccaatttggc gaccggcgag cccggcccgg
      901 cgagtcctcg gggccgctac ggaccgccgc gaataaagaa cccgagcagg agcgtcagga
      961 ccagcccgac gatgcccagc accagtcccg tccctcgtga gggcgagcgg gcgggcgccg
     1021 cggcgcgggc ccagaacccg agcacgcacg ccgccaccgc ggcgaaccac gctccgtgca
     1081 cgctctgcac ggccgcggcg aacgagctac cgtccgggct gaccgcatcg atgatgcgcg
     1141 gcaagaacaa cagcagcacc atcagcgcca cacatatgat gccggcgagc agcagccgcg
     1201 tcgcccgcaa ctcgctcgcc gccgcgagct cgccagatcg atcgttcatg ttgtccatac
     1261 ctgttcgcta agaatgccag ttcgccgcga tggccatcac catggcgatg gccgtggcag
     1321 tcccgagcac gaagcctgcg agcacgagca acagcccatg ccggatcgcg cggtgatcga
     1381 gccgccgtgg cccacccggc tcgctgcgca cgccgacccc gccgccgacc gccgcgagca
     1441 ggcccacgag gccggtggcg agcgccatgc caccggccac atacaacgtc gtcacgatcg
     1501 tggtggcgaa gcagccgatc accgcgacga gcgaaaggca cgcgagccac acgccgagct
     1561 tcacgagctg ctgcggcccc gtccccgaga gcggcgcctc gtcactcgcg cgcacatccg
     1621 cccgtccggt gtcctcgttc atcgcgcacg agcctatcat gaaccggcgt ccgggcgagc
     1681 gagccgcgtg ctcgcacgca accgccctcc gtacgttcat cgggtcgcgc agcgagcccc
     1741 tggcccgccc ctcgtggccc gggcggcgct gatcgtcacg ctcttt
//
LOCUS       DASWNJ010000596         2005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_435729, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000596 DASWNJ010000000
VERSION     DASWNJ010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435729"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /locus_tag="VGB85_09985"
     CDS             <1..1242
                     /locus_tag="VGB85_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134401.1"
                     /translation="AFGTAGLALGAGVMITPAYAYCPCYTLSSSSNDQACGIEAVEGD
                     NPSPEQWNEIFALVAQGAAAWGDQGPSVPDIGQGCGKPNPLVSVPARFPCELLKGIAM
                     QETGWRQFCAPDTPNDQAGKSSRTIISFDCGYGIGQVTSGMHKGEAPDFDRQRVAAEP
                     IYNMATGTRILAEKWKATECVGDNQPTVIEHWYSATWAYNGLAYVNNPNNPNYDAFRG
                     VWDPQQGGAAPYQEKVFGWIEHPNAGWYEAVAPAYPNLGEIGNDGSPGELSEPSCASP
                     TDCANQRPTHPTACDGAEPETTGEPGSTGEPGSTGEPGTTGATTTTTTTTGSPTTDAT
                     TLPETGTHETDSEITESYVPTEQGTAGGSSTGSPGGSTDGARGDGDDGCGCSGAPVPS
                     DMWLLLLPLLRRRRRASHARG"
     gene            1229..>2005
                     /locus_tag="VGB85_09990"
     CDS             1229..>2005
                     /locus_tag="VGB85_09990"
                     /inference="COORDINATES: protein motif:HMM:NF018533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEY0134402.1"
                     /translation="MPEGDTLFRIARTLTQALVGRPFTAVESTVPAIAPAQLVGRTTI
                     AVEAHGKNLMIRLDDGRTIYSHLRMTGSWHVYRPGERWQKPRSGARLVLETADIHAVC
                     FYAPVIELLAPGGEQRHAVLTQLGPDVLAPDFNIEAAARRLDDPRPIGEVVMDQRVIA
                     GIGNVYKSELLFLERIDPWRPAATLGLAERTALMQHTVKLMLPNVRPGAGMRTTRASL
                     GGPRQWVYGRAGEECMVCGMIIAMRRQGAAGRSTYYCSRCQ"
BASE COUNT          339 a          698 c          707 g          261 t
ORIGIN      
        1 gccttcggga cagccggcct ggctctcggg gcaggtgtga tgataacgcc ggcatacgcc
       61 tattgcccgt gttatacgct gtcgagctcg agcaacgatc aggcctgcgg gatcgaggcg
      121 gtcgagggcg acaatccgag cccggagcag tggaacgaga tcttcgcgct ggtcgcccag
      181 ggcgcggcgg cgtggggcga tcaggggccg agcgtgcccg acatcggtca gggctgcgga
      241 aagccgaacc cgctcgtatc ggtgcccgcg cgcttcccgt gcgagctgct caagggcatc
      301 gcgatgcagg agaccggctg gcggcagttc tgcgcgcccg atacccccaa tgatcaggcg
      361 ggcaagtcct cgcgcacgat catctcgttc gactgcggct acggcatcgg ccaggtgacg
      421 agcggcatgc acaagggcga ggcccccgac ttcgaccgcc agcgcgtcgc cgccgagccg
      481 atctacaaca tggcgaccgg cacgcggatc ctcgcggaga agtggaaggc gaccgagtgc
      541 gtcggcgaca accagccgac cgtgatcgag cactggtaca gcgcgacctg ggcctacaac
      601 ggcctcgcct acgtcaacaa cccgaacaac cccaactacg acgcgtttcg cggcgtgtgg
      661 gacccgcagc agggcggcgc cgcgccgtat caggagaagg tgttcggctg gatcgagcac
      721 ccgaacgccg gctggtacga ggccgtcgcg cccgcgtacc cgaacctcgg cgagatcggc
      781 aatgacggca gcccgggcga gctgtccgag cccagctgcg cctcgccgac cgactgcgcc
      841 aatcagcgtc cgacacaccc gaccgcgtgc gacggcgccg agcccgagac gaccggcgag
      901 cccgggagca ccggcgaacc gggcagcacc ggcgagcccg gcacgaccgg cgcgacgact
      961 acaaccacca cgacgaccgg ctcgccgacg accgacgcga cgacgctgcc cgagaccggc
     1021 acccacgaga ccgattcgga gatcacggag agctacgtgc cgaccgagca aggaactgcc
     1081 gggggcagca gcacgggcag ccccggcggg agcacggacg gcgcgcgcgg cgacggcgac
     1141 gacggctgcg gctgcagtgg cgcgcctgtc ccgtcggaca tgtggctatt gctgttgccg
     1201 ctgctgcgtc ggcggcgacg agcgagccat gcccgagggt gacacactct tccggatcgc
     1261 ccgcacgttg acgcaggcgc tcgtcggccg gccgttcacc gcggtcgagt cgaccgtgcc
     1321 cgcgatcgcg ccggcgcagc tggtcggccg cacgacgatc gcggtcgagg cgcacggaaa
     1381 gaacctgatg atcaggctcg acgacggccg gacgatctac agccatttgc gcatgaccgg
     1441 atcgtggcac gtctaccgcc cgggcgagcg ctggcaaaag ccgcggtccg gcgcgcgcct
     1501 ggtgctcgag accgccgata tccacgcggt ctgcttttac gccccggtga tcgagctgct
     1561 cgccccgggc ggtgagcagc gccacgcggt cctgacccag ctcggccccg acgtgctcgc
     1621 gcccgatttt aatatcgagg ccgcggcccg ccgcctcgac gacccgcgcc cgatcggcga
     1681 ggtcgtgatg gatcagcgcg tcatcgccgg gatcggcaac gtctacaagt ccgagctgct
     1741 gttcctcgag cgcatcgacc cctggcgccc ggccgcgacc ctcggcctgg ccgaaaggac
     1801 agccctgatg cagcacaccg tcaagctcat gctccccaac gtgcgcccgg gggccggcat
     1861 gcgcacgaca cgggcctcgc tcgggggtcc gcgccagtgg gtgtatggca gggcgggcga
     1921 ggaatgcatg gtgtgtggga tgatcattgc gatgcgcagg cagggggctg cggggcggtc
     1981 gacgtattat tgttcgcggt gtcag
//
LOCUS       DASWNJ010000597         4151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_223029, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000597 DASWNJ010000000
VERSION     DASWNJ010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223029"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VGB85_09995"
     CDS             <1..682
                     /locus_tag="VGB85_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134403.1"
                     /translation="DATAPPGYRRAVARRGRAAAEALFTTTTDDSRLAHLCTALELTR
                     TYQAELVESEEDRRAVPVDLARLEAQAITANAPCARPPPPPPLHTEPPPAASPRPAPE
                     PLPTLPIAARRSRLQLAAGAGLVTTGVGLVAGFAGCFAARVPEAQRVVVLDQQATAAG
                     RDLTDDELHELLDAEARYVRLSNTGKALGVVGGLALLAGVLVLALPARASRRVQARAV
                     GAGFHIKF"
     gene            complement(672..1112)
                     /locus_tag="VGB85_10000"
     CDS             complement(672..1112)
                     /locus_tag="VGB85_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134404.1"
                     /translation="MAISVSPYQTSTSTTSALAFNSRLTAPGSWIAAGSTENAKIWGA
                     AFSGPPVELEATSSWPVLVMAGSVVAVLVVTPPVVTASVVVTMPGDPVLVCSAPVGSG
                     ASLVMSKPGECSVQPARDTRARARVGRNIDIEDTPDGADTRVKT"
     gene            1114..2421
                     /locus_tag="VGB85_10005"
     CDS             1114..2421
                     /locus_tag="VGB85_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134405.1"
                     /translation="MPAAEFPYRFDVTSQSMCDGSQAFTATVRDAQGLTATSLPAELF
                     CQLPAPGSQVYTRTLEGSASATGTAIALLPDGGVLAGGSLDGCMMLWRLDAAGDPVAG
                     WPKTVADWTLVADLDAKESRATAVAVDPTGALIVAGYFKNGLAFRRYVAKLGDKGTLL
                     WEDPGQVDGEEIMGLAVSPAGDIVTAGSLRTSPLDQPPLYDAMTWGYPDGYPYKAKRW
                     EKLFTKPAADPQLDPDNQDSERMRGVLALPGGRFLAFGERDYSDDIKNLYTRTTMFQV
                     TGDGELVGETWTSLGSQYANDAALAGTLTDTGFALTGWCRFEGPGALQQLCIEIFDVN
                     GNLDQIYVEPWPTQTAGLGVAQDRMQRLVIAGYKSKPGDLDAWVFASLGAGYPRAWEQ
                     PPLEQGAWDFATGIVCEAWGKCTWVGTTTINGHAAVVVSQRYP"
     gene            complement(2425..3285)
                     /locus_tag="VGB85_10010"
     CDS             complement(2425..3285)
                     /locus_tag="VGB85_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134406.1"
                     /translation="MELPLDFGSPGPAGCLGKIDFLFVISNSNTMAPRQAQVLTIFPA
                     FLDSLTQEFADFDSHIMVVETDGGWFMGDCAHCGPGCDPNGELPWCGADLDACDSTMG
                     AGVTFPAGKESSAQRCPLAEGRYITSKDPDPKAAFKCIAKVGSGGGVPFPADAMVAAL
                     SWPLLGTHGYPPGCNQGFLRDDALLVVTIISDVYDAKSSGPAWAWRKALMEAKNQDAA
                     AFQVLVITTDVDTPNGLCGEYTPDVNRLRTFVELTDGLIGSICADDYGQFFKDAAAAI
                     LERCKTYVPQ"
     gene            complement(3713..>4151)
                     /locus_tag="VGB85_10015"
     CDS             complement(3713..>4151)
                     /locus_tag="VGB85_10015"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0134407.1"
                     /translation="SARLMRGALLVMLTLLACEASTPTTVARPASVTALAQAEHRRKG
                     DAADIHVLARGENAFLGKLEMAPGGEVPEHRDPTEEYIHILEGGGVFKIDGQAHQVGP
                     GSTIYMPPGALVSFKNGDARLVAIQVFAGPGPAAKYEAWTPVQ"
BASE COUNT          672 a         1472 c         1385 g          622 t
ORIGIN      
        1 ggatgcgacg gcgccgcccg ggtaccgccg cgccgtcgcc cggcgaggtc gggcggcggc
       61 cgaggccctg ttcaccacga ccacggacga ctcacgcctg gcccatctgt gcaccgccct
      121 tgaactgacg cggacttacc aggcggagct ggtcgagtcc gaggaagacc gccgcgccgt
      181 ccccgtggac ctggcgcggc tcgaggcgca ggcgatcact gcgaatgctc catgcgcccg
      241 gccgccaccg ccgccgccgc tccacaccga gcctcccccg gcagcgagcc cacgcccggc
      301 gccggagcca ttgccgacgc tgccgatcgc cgctcgccgg tcgcgacttc agctcgccgc
      361 gggagccggc ctggtgacga ccggcgtcgg cctcgtcgcc ggcttcgcag gctgtttcgc
      421 ggctcgcgtg cccgaggcac agcgcgtcgt cgtgttggac cagcaggcga ccgccgcggg
      481 ccgcgatctc actgacgacg agctccacga gctcctcgac gccgaggccc gttacgtccg
      541 gctgtcgaac acgggcaagg cgctcggcgt ggtgggcggc ctggccctgc tcgccggtgt
      601 gctcgtgctg gcactgcccg ctcgcgcgtc ccgccgggtc caggcgcgtg cagtgggggc
      661 tggcttccac atcaagtttt gacgcgcgta tccgctccat caggagtatc ttcgatgtcg
      721 atgttccgcc cgaccctcgc cctggccctc gtgtccctcg cgggctgcac cgagcactcc
      781 ccgggttttg acatcaccaa cgaggcgccg gacccgacag gcgcggagca aacgagcacc
      841 gggtctcccg gcatggtcac caccaccgat gcggtgacga cgggcggcgt gacgacgagc
      901 accgcgacga cggacccggc catgaccagc accggccacg acgaggtcgc ctcgagctcg
      961 acgggcgggc ccgagaatgc agcgccccag atcttcgcgt tctcggtcga accggcggcg
     1021 atccacgagc ccggcgcggt aagtcttgag ttgaacgcga gcgccgacgt cgtcgacgtc
     1081 gatgtttggt acggcgagac gctgatcgcc accgtgcccg ccgccgagtt cccctaccgc
     1141 ttcgacgtga cgtcgcagtc gatgtgcgac ggctcgcagg cgttcaccgc caccgtccgc
     1201 gacgcgcagg ggctcacggc gacatccctc cccgcggagc tgttctgcca gctccccgct
     1261 ccgggcagcc aggtctacac ccgcacgctc gagggcagcg cctcggccac cggaaccgcg
     1321 atcgcgttgc taccggacgg gggtgtgctg gccggcggct ccctcgacgg atgcatgatg
     1381 ctgtggcggc tcgacgccgc aggcgaccct gtggcgggct ggcccaaaac ggtcgcggac
     1441 tggaccctgg tggcggacct cgacgccaag gaatcccgcg cgaccgcggt cgcggtcgac
     1501 cccacgggcg cgctcatcgt cgcgggttat tttaaaaatg ggctcgcgtt tcgccgctac
     1561 gtcgccaagc tcggcgacaa gggcacgctg ctgtgggagg accccggcca ggtggacggc
     1621 gaggagatca tggggttggc tgtgtctccg gccggcgaca tcgtgaccgc agggtcgctg
     1681 cgaacgagcc cgctggacca gccgcctctt tatgacgcga tgacgtgggg ctacccggac
     1741 gggtacccct acaaggcgaa gcgctgggaa aagctcttca cgaagcccgc cgcggacccg
     1801 caactggacc cggataacca ggacagcgaa cggatgcgcg gggtcctcgc gcttccaggc
     1861 ggccgctttc tcgccttcgg cgagcgcgac tattcggacg atatcaagaa cctgtacacg
     1921 cgcacgacga tgtttcaggt gaccggcgac ggcgagctgg tcggcgagac gtggacgtcg
     1981 ctcggcagcc agtacgcaaa cgacgccgca ctcgcaggga cgctcaccga cacaggcttc
     2041 gcactgacgg ggtggtgtcg cttcgagggt ccaggcgctc tacagcagct gtgcatcgaa
     2101 atctttgatg tcaatggcaa cctggaccag atctatgtcg agccatggcc gacgcagacc
     2161 gccggcctgg gcgtcgcgca ggaccgcatg cagcggctcg tgatcgccgg gtacaaatcg
     2221 aagccgggcg atctcgatgc ctgggtcttt gcgtccctcg gcgccggata cccgcgcgcc
     2281 tgggagcagc cgccgctcga gcagggcgcg tgggacttcg caaccgggat cgtctgcgag
     2341 gcgtggggca aatgcacctg ggtcggcacg acaacgatca acggccacgc cgcggtcgtg
     2401 gtcagtcagc gctacccgtg acactcactg tggcacgtag gtcttgcagc gctccaggat
     2461 cgcggctgcg gcgtccttga agaactgccc atagtcgtcg gcgcagatgc tcccgatcaa
     2521 gccatccgtg agctccacga aggtgcgaag ccgattgacg tcgggggtgt actcaccgca
     2581 caggccgttg ggagtgtcga cgtccgtcgt gatcacgagg acctggaaag ccgcggcgtc
     2641 ctgatttttg gcctccatca gcgctttgcg ccaggcccac gcgggaccgg acgacttggc
     2701 gtcgtacaca tccgagatga tggtgacgac aagtagcgcg tcgtcccgca aaaacccctg
     2761 gttgcagccc ggcggatagc cgtgcgtgcc gagcagcggc cagctgagcg ccgcgaccat
     2821 cgcgtcggca ggaaaaggga caccaccgcc gctcccgacc tttgcgatac acttgaaggc
     2881 cgccttcgga tcgggatcct tgctggtgat gtaccggccc tcagccagag ggcagcgctg
     2941 ggcgctcgac tccttgcctg caggaaatgt aacccccgcg cccatcgtcg agtcgcacgc
     3001 gtccaggtcg gctccacacc acggcaactc cccgttcggg tcacagcctg gcccgcagtg
     3061 cgcacaatcc cccataaacc aaccgccgtc cgtctcgacg accatgatat gcgagtcgaa
     3121 atccgcgaat tcctgcgtga gcgagtcgag aaaggccgga aaaatcgtca acacctgggc
     3181 ctggcgcggc gccatcgtat tggaattgct gatgacgaac aggaaatcga tcttgccgag
     3241 gcagccggct ggccccgggg acccgaagtc caggggcagc tccatgccag tgctcgtgct
     3301 accgccagcc tcggacgcgc caccggacgc cgagcttgtg gaactggtgg agctggccgc
     3361 ctcgctggaa gcgctggagc tcccggagga agtgcccggg tccggcgccg aggtcggccc
     3421 cacgccgaag ccggagaccc ccggcggccc ctggttacac gccagcgaga ggcacagcgc
     3481 gagggaaggc agtagcccgc gcatgcgcgc gagcgacgcc tgtgatgacg ggcctggcct
     3541 catgaggcgc attttagcgc ttccccgatt cacgcgccac aacagctacc ttgggcgtct
     3601 caagcgtcac tcgggaaggg agacgcccgg cgaggcagac tggcacccgc agcacgccgg
     3661 ccacaggaca tcggtcgtgc gagctgctgc tcgccaagct gggggagccg actcactgca
     3721 ccggcgtcca cgcctcgtac ttggccgccg gtccgggccc cgcgaacacc tggatcgcca
     3781 ccagccgcgc gtccccattt ttaaaagaaa ccagcgcgcc gggtggcatg tagatcgtcg
     3841 agcccggccc gacctggtgg gcctgcccgt ctatcttaaa caccccgccg ccctcgagga
     3901 tatggatgta ctcctcggtc gggtcgcggt gctccggcac ctcgccgccg ggcgccatct
     3961 cgagcttgcc gaggaacgcg ttctcgccgc gcgcgagcac gtggatgtcg gccgcgtcgc
     4021 ccttgcgccg gtgctcggcc tgcgcgagcg cggtcaccga cgccggtcgt gcgaccgtcg
     4081 tcggcgtgct cgcctcgcag gcgagcagcg tcagcatcac cagcagcgcc ccgcgcatca
     4141 gccgagcact t
//
LOCUS       DASWNJ010000598         6011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_541379, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000598 DASWNJ010000000
VERSION     DASWNJ010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541379"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VGB85_10020"
     CDS             complement(<1..710)
                     /locus_tag="VGB85_10020"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY0134408.1"
                     /translation="MDKIVIHGGQRLSGEIRILGAKNAALPIQCAALLTDTPTTLRHV
                     PRLSDVKTMGELLVGLGCTVEGDRTLVIDPAGVAAGPLEAPYELVKTMRASVTVMGPL
                     LARYGKCRVSLPGGCAIGARPIDQHLRGLEALGAKIQMAHGYVELKARRLKGTQFTFD
                     VITVGGTQNVMMAAALARGTTRLENVAREPEVQELAVVLNKMGAKIHGAGTSAMEIEG
                     VDTLHGIDHAILPDRIEAG"
     gene            complement(717..1712)
                     /locus_tag="VGB85_10025"
     CDS             complement(717..1712)
                     /locus_tag="VGB85_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134409.1"
                     /translation="MTTRPASLALLVLLATTACAPGEPSGVAPEVLQTDFDLSPTPAT
                     TRVATLAWQPPVCPQVYRVRIDETYPPGLEKMLRTQAEHSESLMAVGAEPDSRADTWP
                     PGPIPQDRVFAGRVVFRGPKTQQKPLHREFALSAALAGPASPDAPCYERTWDPVEDAV
                     ALAWPQLPARLAAVGETWRGARVEARCNRSACVDTVTRGGGRENHFRPCTTMSWRERL
                     DGLFELGDDRVAVISSFWSDGHPLDEGLWSERTAVVSVEHGRLLHSQTFIHHTYTGIE
                     REVRVDAIDSCPGGLVAAGWSPDAAASDGRETLLSALANSGDKPKPADKTRDGAR"
     gene            complement(1716..2009)
                     /locus_tag="VGB85_10030"
     CDS             complement(1716..2009)
                     /locus_tag="VGB85_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134410.1"
                     /translation="MPPVDHKTMFEIYREQDYNRAFRYVFFTDLDEHNRGKEIARAAA
                     GETVFSGFLSDERKADARVAVEAIVEELNAMDEDAASLSDAEFRRRLGPYLVT"
     gene            2383..3237
                     /locus_tag="VGB85_10035"
     CDS             2383..3237
                     /locus_tag="VGB85_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0134411.1"
                     /translation="MLSRLSPYLLAFGVVSLGLSTTACKAPKYPACKKDKHCNVDLDE
                     KCVDGQCQNCKVDTDCNGKGPNGANWKCAEFRCQDPAAIVGGGGADGIGGPCTQTIDC
                     SGGTVCKAGACAACNDNIDCQPGTCDIGTGRCSSPGDGPAGTQCQTDDQCAMDEICDN
                     GNCVFSGVSQGGGSNPCPETETLFFGSDSPNLEPADQEKLKNLANCMKTNATRAVILE
                     AHADSRGTEDYNIMLTDKRGNNVKEFLKELGVDPTRMNVVSKGDLEATGTDDASMSKD
                     RRVQFVWQ"
     gene            3255..4229
                     /gene="bamD"
                     /locus_tag="VGB85_10040"
     CDS             3255..4229
                     /gene="bamD"
                     /locus_tag="VGB85_10040"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEY0134412.1"
                     /translation="MSHTTSIPGLPTRNALRGAILVLAAVLGPAAATGCLTLKSEHND
                     LAARVTKIDQSSGDRGAQLDEKLAEADRKLAELQAKLDQAETLLRGSQAGIGARMDRV
                     EEEVAQLRGAAENAELVSSSSQHSMQELRTEVDQRLTKLEEKLNEATNIPEGKDELWS
                     EAERQLQKKNYKLARQLWRTFISRYPGDPKLPQANFQVGLTFFSERDYKSALGQFYNI
                     IQQYSEDPIVSDALYYSGLGFAKLGQCKNATAYFEALLRPKSGASPQYQKAARDQIEI
                     LKKDNGDLCFDREDANAGAAAKRGVQETQKDPAKASTKATTPAKGKAK"
     gene            4536..>6011
                     /locus_tag="VGB85_10045"
     CDS             4536..>6011
                     /locus_tag="VGB85_10045"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0134413.1"
                     /translation="MNAVGPQSVAGQATVPGLAGMTLPARIGRFAVLEPLGSGGMGLV
                     CAAYDSELDRKVAIKLLRPQTATAGKIALAQARLLREAQAMARLAHPNVVTIHEVGTH
                     GDQVFIAMEFLFGLTLKRWLRRARRPWQEVLAVFLQAGRGLAAAHAAGLIHRDFKPDN
                     VMVTEDTRTGKLDRVRVFDFGVVQETREAAVRQSSHSLRTPSPDGRRVTTPEPERRIE
                     RSSSEDLPSEPLRRILRSSLPLLGEVTPMDSQEWVPPSIDSSLPGDEIYATGLTAAGA
                     LVGTPLYMAPEQYDGTADARSDQFSFCAALYEALYRRRPFGGDTAARLQQAKLMGAIR
                     RPPRNHEVPAWLHRIVIRGLASNPARRFPSMDALLAELARDHGHRTPRALIGLAVALG
                     LVGLGAALAWSLARAPGDPCISAAASLAPAWNPDRREQIQAAFRGSGRPYAHSTWSTI
                     EPRMDSYVKAWVEQRVGLCRAAGDPHQGHVLALRHLCLEQRR"
BASE COUNT          925 a         2171 c         2071 g          844 t
ORIGIN      
        1 ccggcctcga tgcggtcggg caggatcgcg tggtcgatgc cgtgcagggt gtcgacgccc
       61 tcgatctcca tcgccgaggt gcccgcgccg tggatcttcg cgcccatctt gttgagcacg
      121 accgcgagct cctggacctc gggctcgcgc gcgacgttct cgaggcgcgt cgtgcccctg
      181 gccagcgcgg cggccatcat cacgttctgc gtgccgccga cggtgatcac gtcgaaggtg
      241 aactgggtgc ccttgaggcg tcgggccttg agctcgacgt agccgtgggc catctggatc
      301 ttcgcgccga gggcctcgag gccgcgcagg tgctggtcga tcgggcgcgc gccgatggcg
      361 cagccgcccg gcagcgacac gcgacacttg ccgtagcggg ccagcagcgg gcccatgacg
      421 gtcaccgagg cgcgcatggt cttgaccagc tcgtagggcg cctcgagcgg ccccgcggcg
      481 acgccggccg ggtcgatgac gagggtgcgg tcgccctcga cggtgcagcc gaggccgacg
      541 agcagctcgc ccatcgtctt gacgtcgctg agcctgggca cgtggcgcag ggtggtcggc
      601 gtgtcggtca gcagcgcggc gcactggatc ggcagggccg cgttcttggc cccgaggatg
      661 cggatctctc cgctcagtcg ctggcccccg tgtatgacga tcttgtccat ccgctcctaa
      721 cgcgccccgt ccctggtttt atccgccggc ttcggcttat ccccggagtt ggcgagcgcg
      781 gacagcagcg tctcgcgccc gtccgacgcg gccgcgtcgg ggctccagcc ggcagccacc
      841 aggcccccgg ggcagctatc gatggcgtcg acgcggacct cgcgttcgat gccggtgtac
      901 gtgtgatgaa tgaaggtctg actgtgtagc agacgcccgt gctcgacgga gacgaccgcg
      961 gtgcgctcgc tccacaggcc ctcgtcgagc gggtgcccgt cgctccagaa gctcgagatc
     1021 acggcgactc ggtcgtcgcc gagctcgaac aggccgtcga ggcgctcgcg ccaggacatc
     1081 gtcgtgcacg gccggaagtg attctcccgg ccaccgccgc gggtcacggt gtcgacgcag
     1141 gcgctgcggt tgcagcgggc ctcgactctc gcgccgcgcc aggtctcgcc gacggcggcg
     1201 aggcgggctg gcagctgcgg ccaggcgagc gcgacggcgt cctcgaccgg gtcccaggtc
     1261 cgctcgtagc acggggcgtc cggcgaggcc ggcccggcga gcgcggccga cagcgcgaac
     1321 tcgcggtgca gcggcttttg ctgggtcttc gggccgcgaa acaccacgcg cccggcgaac
     1381 acccggtcct gcgggatcgg gccggggggc caggtgtcgg cgcggctgtc cggttcggcg
     1441 ccgacggcca tcaagctctc gctgtgctcc gcctgcgtgc gcaacatctt ctccaggccg
     1501 ggcgggtagg tctcgtcgat gcgcacgcga tacacctgcg ggcacaccgg cggctgccag
     1561 gcgagggtgg cgacgcgggt ggtcgccggc gtcggtgaca ggtcgaagtc ggtttgtaac
     1621 acctcgggcg cgaccccgga cggctcgccc ggggcgcagg cggtcgtcgc cagcaaaacc
     1681 agcagcgcaa gcgaggcggg gcgcgtggtc atcgttcacg tcaccaggta cggcccgagg
     1741 cggcgccgga attcggcgtc gctgaggctc gcggcgtcct cgtccatcgc gttgagctcc
     1801 tcgacgatcg cctcgacggc gacgcgggcg tcggccttgc gctcgtcgga gaggaacccg
     1861 gaaaataccg tctcgccggc ggccgcccgg gcgatctcct tgccgcggtt gtgctcgtcg
     1921 aggtcggtga agaacacgta gcgaaacgcg cgattatagt cctgctcgcg gtagatctcg
     1981 aacatcgtct tgtggtcgac cgggggcatg ggcgcgaacc atgccagagc tggcgacgaa
     2041 gcaaaagccg gcggcacttc gcggccgcct cctcgttccc ccgaccgatc gcgatcgcgg
     2101 tccgccttcg tgtttgcatg cgtatgcagg tcgttcgcgc cggctgcgca ggaccgccgg
     2161 ctccacgtgt cttccgcgct cgccagcggc ccaagtttcc cggcccggac ccacgagccc
     2221 tccagggggc cgccgtcgcg ggcgcgcaga cgggccctgt ggttgacaca gcgcgccggg
     2281 cgaggctatg ccttggcgca cctcggcgcc ggcttttggt cggcgtctac cgtctgcgtc
     2341 agaaacttcc ctgcactccc ggagccgcct aacatgcgcg ccatgctgtc ccgcctgtcc
     2401 ccctatctgc tcgccttcgg ggtcgtttcc ctgggcctgt cgaccacggc gtgcaaggcg
     2461 cccaagtacc ccgcgtgcaa gaaggacaag cactgcaacg tcgacctcga cgagaagtgc
     2521 gtcgacggtc agtgccagaa ctgcaaggtc gacaccgact gcaacggcaa ggggcccaac
     2581 ggcgccaact ggaagtgcgc cgagttccgc tgccaggacc cggctgccat cgtcggcggc
     2641 ggcggtgccg acggcatcgg cggcccgtgc acccagacga tcgactgctc cggcggcacc
     2701 gtgtgcaagg ccggcgcctg cgccgcttgt aacgacaaca tcgactgcca gcccggcacc
     2761 tgcgacatcg gcaccggtcg ctgctcctcc cccggcgacg gcccggccgg cacccagtgc
     2821 cagaccgacg accagtgcgc gatggacgag atctgcgaca atgggaactg cgtgttctcg
     2881 ggcgtgtccc agggcggcgg ctcgaacccc tgcccggaga ccgagaccct gttcttcggc
     2941 tccgacagcc cgaacctcga gcccgccgac caggagaagc tcaagaacct ggccaactgc
     3001 atgaagacca acgccacccg cgcggtcatc ctcgaagccc acgccgactc gcgtggcacc
     3061 gaggactaca acatcatgct gaccgacaag cgcggcaaca acgtcaagga gttcctcaag
     3121 gagctcggcg tcgacccgac gcgcatgaac gtcgtgtcga agggcgacct cgaggcgacc
     3181 ggcaccgacg acgccagcat gtccaaggac cgccgcgtcc agttcgtgtg gcagtgagcc
     3241 gattcgaccg gatcatgtct cacacgactt cgatccctgg cctcccgacg cgcaacgcgc
     3301 tgcggggggc catcctcgtg ctcgccgccg tgctcgggcc ggcggccgcg accggctgcc
     3361 tgacgctcaa gtcggagcac aacgacctcg ccgcccgcgt cacgaagatc gaccagtcga
     3421 gcggtgaccg cggcgcccag ctcgacgaga agctcgccga ggccgaccgc aagctcgccg
     3481 agctgcaagc caagctcgac caggccgaga ccctgctgcg cggcagccag gccggcatcg
     3541 gcgcccgcat ggaccgcgtc gaggaggagg tcgcgcagct gcgcggcgcc gccgagaacg
     3601 ccgagctggt ctcgtcctcg agccagcact ccatgcagga gctgcgcacc gaggtcgacc
     3661 agcgcctgac caagctcgag gagaagctca acgaggcgac caacatcccc gagggcaagg
     3721 acgagctgtg gagcgaggcg gagcgccagc tccagaagaa gaactacaag ctggccaggc
     3781 agctgtggcg caccttcatc tcgcgctatc cgggcgaccc gaagcttccg caggccaact
     3841 ttcaggtcgg cctgaccttc ttcagcgagc gcgactacaa gagcgctctc gggcagttct
     3901 acaacatcat ccagcagtac agcgaggacc cgatcgtgtc ggacgcgctg tactactcgg
     3961 gcctcggctt cgccaagctc ggccagtgca agaacgcgac ggcctacttc gaggcgctgc
     4021 tgcgaccgaa gtccggcgcg agcccgcagt accagaaggc cgcccgcgac cagatcgaga
     4081 tcctcaaaaa ggacaacggc gacctctgct tcgaccgcga ggacgccaac gccggcgcgg
     4141 ccgccaagcg cggcgtgcaa gagacacaga aggatccggc caaagcgtcc acgaaggcga
     4201 cgacgccggc caagggcaag gccaagtaat cgcgcactgc atgcgcactt gtgcgcatgc
     4261 agtgcgctcg cgcgcctgca caggtgcagg cgcggggatc accggcacca gagtgatttg
     4321 cgacggctgc gggatgtgag tactcggggt tatcctggtg ggccgtggca ttccccaccc
     4381 agcgcgaccc cggcggatcg acgagcgatt cgccgatcgc gtccgtgacc gcgtcgagct
     4441 cgatgagcac gtcgagtgcg tcaggttcgt cgggctcgtc gagcacagcc gggttggtcg
     4501 gcttcgcaag ctctgcgtca tcgtccttga acgccgtgaa cgcggtcgga ccgcagagcg
     4561 tggcggggca ggcgacggtc ccggggctcg ccggcatgac cctgccggcc cgcatcggcc
     4621 gcttcgccgt gctcgagccg ctcggctcgg gcggcatggg cctggtctgc gccgcctacg
     4681 acagcgagct cgaccgcaag gtggcgatca agctgctgcg accgcagacg gcgaccgccg
     4741 ggaagatcgc gctggcgcag gcccgattgc tgcgcgaggc ccaggcgatg gcccgcctcg
     4801 cccaccccaa cgtcgtcacg atccacgagg tcggcaccca cggcgatcag gtcttcatcg
     4861 ccatggagtt cctgttcggc ctgaccctca agcgctggct gcgacgcgcg cgacgaccct
     4921 ggcaagaggt cctcgccgtg ttcttgcagg ccggccgcgg cctcgctgcc gcccacgctg
     4981 cgggcctgat ccaccgcgac ttcaagcccg acaacgtgat ggtcaccgag gacacgcgca
     5041 ccggcaagct cgaccgcgtc cgcgtgttcg acttcggcgt ggtccaggag acgcgcgagg
     5101 cggcggtccg ccagagctcg cacagcctgc gcacgccgag cccggacggc cgccgcgtca
     5161 ccacacccga accagagcgg aggatcgagc gctcgtcgag cgaggatctg ccgagcgaac
     5221 cactgcgacg gatcctccgc tcgtcgctgc cgctgctcgg cgaggtgacg ccgatggact
     5281 cgcaggagtg ggtcccgccg agcatcgaca gcagcctgcc cggcgacgag atctacgcga
     5341 ccgggctgac cgccgccggc gctctggtcg gcacgccgct gtacatggcg cccgagcagt
     5401 acgacggcac cgccgacgcg cgcagcgacc agtttagctt ctgcgccgcg ctgtacgagg
     5461 cgctgtaccg gcggcggccg ttcggcggcg acacggcggc gcgcctgcag caggccaagc
     5521 tgatgggcgc gatccgccgg ccgcccagga accacgaggt cccggcctgg ctgcatcgca
     5581 tcgtgatccg cggcctcgcc agcaaccccg cgcgccggtt cccgtcgatg gacgcgctgc
     5641 tcgccgagct cgcgcgcgac cacggacacc ggaccccacg cgcgctgatc ggcctcgccg
     5701 tcgcgctggg cctcgtcggc ctcggcgctg cgctggcgtg gagcctcgcg cgcgcgccgg
     5761 gtgacccgtg catcagcgcc gccgccagcc tcgccccggc ctggaacccc gaccggcgcg
     5821 agcagatcca ggcggcgttt cgcggctcgg gccgacccta cgcgcacagc acctggtcca
     5881 cgatcgagcc gaggatggac tcgtacgtga aagcctgggt cgagcagcgc gtcgggctct
     5941 gccgcgccgc cggggacccg caccagggcc acgtgctcgc gctgcgacac ctctgcctcg
     6001 agcagcgccg c
//
LOCUS       DASWNJ010000599         1610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_46498, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000599 DASWNJ010000000
VERSION     DASWNJ010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46498"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..>1610
                     /locus_tag="VGB85_10050"
     CDS             98..>1610
                     /locus_tag="VGB85_10050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEY0134414.1"
                     /translation="MPWHDEYLAAVHDVQRGLGEQVELSTLISAILWHASQYFGDLSS
                     AVYLYNGNDLVALAETGGLAQRLGPRLPVDDPSLLGEAWRSNPPWPEAHAGPPGPGHG
                     PIAAVPIATDEQRLGAMIVAMAPGPDARRFSDDDLLAMRQFAGLVAVSAVNARMYFAA
                     RQELAERRRAEEALRESEERFRTLFEYAPEAVVVVDADTGKFVDPNGNAARLFGLSRE
                     ELLAVGPGDMSPPVQPDGSPSAVLALGRVNEALAGRPPVFEWTHRNATGQDIPCEVRL
                     VRMPAAGRNLVRASVTDITERKRSQEALATAKEAAEAANRAKSAFLANMSHEIRTPMN
                     AIIGMTSLLLDTPLARDQRDFVETLRSSCDSLLTLINDILDFSKIEANKLELENNAFE
                     LRGFVESVIDLVAASKLGDKALNLATILDPSVPPRLISDSTRLRQILLNLLGNAVKFT
                     PHGDVTVTVSARQLTEGHGEVPSRYELQFAVRDTGIGIPRDRMDRLFQSFSQID"
BASE COUNT          250 a          631 c          518 g          211 t
ORIGIN      
        1 cccggatcgc ctatcatgtc gccccgtgac ggcctggcag cgatgatccc tcggcgagca
       61 aacaacacgc gccccggcgc cgacgcgaaa aactccgatg ccctggcatg acgagtacct
      121 cgccgccgtg cacgacgtgc agcgcggcct cggcgagcag gtcgagctgt cgacgctgat
      181 cagcgccatc ttgtggcacg cctcgcagta cttcggcgac ctcagctcgg ccgtctatct
      241 ctacaacggc aacgacctcg tcgcgctcgc ggagaccggc ggcctggccc agcggctcgg
      301 gccgcgcttg ccggtcgacg acccgagcct gctcggcgag gcctggcgct cgaacccgcc
      361 gtggcccgag gcgcacgccg ggccgccggg ccccggtcac ggcccgatcg ccgccgtccc
      421 gatcgccacc gatgagcagc gcctcggcgc gatgatcgtc gcgatggcgc ccgggcccga
      481 cgcccgccgc ttcagcgacg acgacctgct ggcgatgcgc cagttcgccg gcctcgtcgc
      541 cgtctccgcc gtcaacgccc gcatgtactt cgccgcccgc caggagctcg ccgagcgccg
      601 ccgggccgag gaggccctgc gcgagagcga ggagcggttt aggaccctct tcgagtacgc
      661 gcccgaggcc gtcgtcgtcg tcgacgccga caccggcaag ttcgtcgacc ccaacggcaa
      721 cgccgcgcgg ctgttcggcc tctcgcgcga ggagctgctc gcggtcggcc ccggcgacat
      781 gagcccgccg gtccagcccg acggcagccc ctcggccgtg ctcgccctcg ggcgcgtcaa
      841 cgaggcgctc gccggccgcc cgccggtgtt cgagtggacg caccgcaacg cgaccggcca
      901 ggacatcccg tgcgaggtgc ggctcgtgcg gatgccggcg gccggtcgca acctcgtgcg
      961 cgcgagcgtc accgacatca ccgagcgcaa gcgcagccag gaggcgctgg cgaccgcgaa
     1021 ggaggccgcg gaggcggcca accgagccaa gagcgccttc ctggccaaca tgagccacga
     1081 gatccgcacg ccgatgaacg cgatcatcgg catgacgagc ctgctgctcg acaccccgct
     1141 cgcgcgggac cagcgcgact tcgtcgagac cctgcgcagc tcgtgcgact cgctgctgac
     1201 gctgatcaac gacatcctcg acttctcgaa gatcgaggcc aacaagctcg agctcgagaa
     1261 caacgccttc gagctgcgcg gcttcgtcga gtcggtgatc gacctcgtcg ccgccagcaa
     1321 gctcggcgac aaggcgctca acctcgccac gatcctcgac cccagcgtgc cgccgcggct
     1381 gatcagcgac tcgacgcgcc tgcgccagat cctgctcaat ttgctcggca acgccgtcaa
     1441 gttcaccccg cacggcgacg tcaccgtcac cgtcagcgcc cgccagctca ccgagggcca
     1501 cggcgaggtc ccgtcgcgct acgagctgca gttcgcggtc cgcgacaccg gcatcggcat
     1561 cccgcgcgac cgcatggacc gcctgttcca gtcgtttagc cagatcgaca
//
LOCUS       DASWNJ010000600         2386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_470070, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000600 DASWNJ010000000
VERSION     DASWNJ010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470070"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1037)
                     /locus_tag="VGB85_10055"
     CDS             complement(72..1037)
                     /locus_tag="VGB85_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEY0134415.1"
                     /translation="MIMAAGFGTRLGELSAQRPKPMLPVCGAPLVRWAALWLRSQGIR
                     EIVINLHHRGEQIEEELGDGSALGLAIAYSREAEILGTGGGLRNARALLDDGHGTPIV
                     AMNGKVILDLDLAPVLALHEARQAEATMVLRADPEAERWGSLRTDADGHVTHLLGKPG
                     PGGKEGGPALMFTGVQVLTPRFLDRIPGEGQQCVVRTAYSQLHAEGGAYAGYVTDEYW
                     WEHSTVERYLQGVANVIEGRARVRYAPGFVRGVDPTAEVDPSARVDPAVWIGPGVRVG
                     ANAEVGPRVQLGAGAIVEPGRRLRDAIVWDGAHVTRDLEREVLTA"
     gene            complement(1088..>2386)
                     /locus_tag="VGB85_10060"
     CDS             complement(1088..>2386)
                     /locus_tag="VGB85_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134416.1"
                     /translation="VRVTDPEGVPHVGPVEVRLLDGEFGAVKANSDAPPVVFQGSTDA
                     QGLLRFDGVLSTDVVRFEVRVAAPVQAPCAKCMDPNTAVGRTMLALAAEAAAKSGATL
                     PGATATAGTGATGTGEGTTGAGATGSTGVTGPATTVTDPATPPGPLGTRKFRLVSFAG
                     AVRVTAEPLALRVGPTLESALEIKARALRAKKAVFVDLHGPDGAWIDTLTPVTGPEPP
                     RAWSAKGLAPGFLQIEAYQFTTSPGESAALARVQLTQGEPDSDAALAPLFVEQRALLA
                     IGRVEKGFDRELERKYLDVIEKAEVAASDVPLARAWLLGTLPVEVLGPPLALSSLPRE
                     QADVAARKQRWATGLRWFLLGGGGVFLLVTLLLIVISHRRAAAKLTGEIHGEGATSAI
                     AHEIRQAQRAVLLRAVGLVLTMAMGLVLTVVVLDKLLWNT"
BASE COUNT          318 a          960 c          781 g          327 t
ORIGIN      
        1 cgcttcagag gcacgatatc acggtgcaaa cggcgttccg cgcgccttat gccagcgcgg
       61 aacgacgcgg ctcaggcggt gagaacttca cgttcgaggt ctcgcgtcac gtgggccccg
      121 tcccacacga tcgcgtcgcg cagacgtcgt cccggctcga cgatcgcacc ggcaccgagc
      181 tgtacgcgtg ggccgacctc tgcgttcgcg ccgacgcgca cgccgggccc gatccacacc
      241 gccgggtcga cgcgtgcgga cgggtcgacc tccgccgtcg gatcgacgcc acgcacgaag
      301 cccggcgcat aacggactcg cgcccgaccc tcgatcacgt tggcgacgcc ttgcaggtag
      361 cgctcgaccg tcgagtgctc ccaccagtac tcgtcggtca cgtaacccgc gtatgccccg
      421 ccctccgcgt gcagctggct gtatgcggtc cgcaccacgc actgctggcc ctcgcccggg
      481 atccggtcga ggaagcgcgg cgtcagcacc tgcacgccgg tgaacatcag cgcgggtccg
      541 ccttccttgc cgccgggacc tggctttccg agcaggtgcg tcacgtgacc gtcggcgtcg
      601 gtgcgcaggc tgccccagcg ctcggcctcg ggatcggcgc gcagcaccat cgtcgcctcg
      661 gcctgccgcg cctcgtgcaa cgcgagcacc ggcgcgagat cgagatcgag gatcaccttg
      721 ccgttcatcg ccacgatcgg cgtgccgtgg ccgtcgtcga gcagggcccg ggcgttgcgc
      781 aggccgccgc cggtgccgag gatctccgcc tcgcgcgagt atgcgatcgc cagcccgagc
      841 gccgagccgt cgccgagctc ctcctcgatc tgctcgccgc ggtggtgcag gttgatcacg
      901 atctcgcgga tcccctggga ccgcagccac agcgccgccc agcggaccag cggcgccccg
      961 cagaccggca gcatcggctt gggtcgctgc gcgctgagct caccgagccg cgtgccgaag
     1021 cccgcggcca tgatcatgcc cctcatccct cgcatcactc gtggggtcgc catggccccg
     1081 cagcttatca ggtgttccat agcagcttgt cgagcacgac gaccgtcagc accagcccca
     1141 tcgccatcgt cagcaccagc ccgaccgccc ggagcagcac ggcgcgctgg gcctggcgga
     1201 tctcgtgggc gatcgccgac gtcgcgccct cgccgtggat ctcgccggtc agcttcgccg
     1261 cggcgcggcg gtggctgatc acgatcagca gcagcgtcac cagcagaaac acgccgccgc
     1321 cgccgagcag gaaccaccgc aggccggtcg cccagcgctg cttgcgggcc gcgacgtcgg
     1381 cctgctcgcg cggcaggctc gacagcgcca gcggcggccc gagcacctcg accggcagcg
     1441 tcccgagcag ccacgcgcgc gccagcggca cgtcgctcgc ggcgacctcc gccttctcga
     1501 tcacgtcgag gtacttgcgc tcgagctcgc ggtcgaagcc cttctcgacg cgaccgatcg
     1561 ccagcaaggc ccgctgctcg acgaacaacg gcgccagcgc ggcgtcgctg tcgggctcgc
     1621 cctgcgtcag ctgcacgcgc gccagcgcgg ccgactcgcc cggcgaggtc gtgaactgat
     1681 aggcctcgat ctgcaggaag cccggcgcga gccccttcgc cgaccacgcg cgcggcggct
     1741 cgggcccggt caccggcgtc agcgtgtcga tccacgcccc gtccggcccg tgcaggtcga
     1801 cgaacaccgc cttcttggcc cgcagcgccc gcgccttgat ctcgagcgcg ctctccagcg
     1861 tcggcccgac gcgtagcgcc agcggctcgg ccgtcacccg caccgcgccc gcgaagctga
     1921 cgagccgaaa cttgcgcgtg cccagcggcc ccggcggcgt cgcggggtcc gtgacggtcg
     1981 tggccgggcc cgtgaccccg gtcgagcccg ttgcacctgc acccgtcgta ccctcgcccg
     2041 tgcccgtcgc accggtgccc gccgtcgccg tcgcgcccgg cagcgtcgcc ccactctttg
     2101 ccgcggcctc ggccgccagc gcgagcatcg tccgaccgac ggccgtgttc gggtccatgc
     2161 acttcgcgca cggcgcctgc accggcgcgg cgactcgcac ctcgaagcgc acgacatccg
     2221 tgctcagcac cccgtcgaag cgcagcagcc cctgcgcgtc ggtcgagccc tggaacacca
     2281 ccggcggcgc gtcgctgttc gcttttaccg ccccgaactc tccgtcgagc agtcgcacct
     2341 cgaccggccc gacgtgcggc acgccctcgg ggtcggtcac gcggac
//
LOCUS       DASWNJ010000601         3842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_46503, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000601 DASWNJ010000000
VERSION     DASWNJ010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46503"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(618..1736)
                     /locus_tag="VGB85_10065"
     CDS             complement(618..1736)
                     /locus_tag="VGB85_10065"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEY0134417.1"
                     /translation="MSAHRPLSLGTATASPGELTYGWYTLADLPTGHVERLPVLLARG
                     REPGPTLWLTANIHGDELTGMGVIHDVVTPALLDDLRGTIVAIPSLNPAGLHTRQREP
                     YLDARDPNRLFPGIDPPGGDPAERARLAETPGVFEAGYAALFAELRASADLYVDLHCF
                     GLQAASFIIRDRILYSDEAELPALGSLLTRLDALCRASGLPVVLEHPAGRYVEKLLHR
                     STSGAALNLARIPAITVELGLIGGIDPAALAAGTTAVLNILKGSGMLPGEPEPVRSVP
                     QPAVDFPVMRESTPRARASGVLRYHVQPGDVFRAGDLLATLTDLHGRKLAEHGELRAE
                     ADGWVMGLARGAICYQGQTVTHVAVRDDGPLLERYPAA"
     gene            complement(1774..>3842)
                     /locus_tag="VGB85_10070"
     CDS             complement(1774..>3842)
                     /locus_tag="VGB85_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY0134418.1"
                     /translation="WIRVGTREIRGLMKSRDDARRTYEAARDAGQTAALLEQNRPNVF
                     AQAVANIQPGETVEVEMHVVQPLRREAGRYGLTLPITVGPRYAPGTPVDAAGNTTQVP
                     DAAKINSPQLGKDVVSCSPVDIQVTIEAGFPVSALRSKYHSLTVAQAGDITRLELARG
                     PARPNRDFSISWQLAGLEPRARLMAQKTADGQGYFTLAVEPPQGFSQEKARPRELVFA
                     LDTSGSMQGEPLDTSVAAVQKALDGMGADDTFQILRFAGDTGQLAPAPLRNTAENRRR
                     GIEYMRNFSGDGGTEMLAGITAALAFKKDPERLRMVMFLTDGYIGNETEIFRRIGEVR
                     GDARLFGLGVGSSVNHYLLEGISRMGRGSVTYVGPGEKVDAVVERFYSYIDRPVLTDI
                     EIDWGGLPVADVVPAALPDLFSGQPVVVYGRFTGEAKGQAKLKGKLGGAPVEMPLRLD
                     FADGAEHTGLRSMWARQKVDALLNYPDSQQSSADEKTVAAVTKLAIEHRVMTQYTSFV
                     AVETKQEKQSDGTLKTVHVPLELPLGVEEIGESYGTIGLGNTGLIGRGGGGGSGSGYG
                     RGYGAGFGGRGTRVPTVRQAKAEVQGSLDKEIIRRIVRAHINEVRYCYNQALARDPNA
                     KGRVAITFTVAADGKVLKAEVSESTMADAAVGTCVATAVKTWTFPKPDGGASVIITYP
                     FVLEPG"
BASE COUNT          541 a         1274 c         1474 g          553 t
ORIGIN      
        1 cgcgggagcg tggttcacga gtgacgacac gaatgaggga acgatgagca aaaaccccgc
       61 gcggtcgcgc ggcgccgacg tccagctgcg ggcgcgacaa aggctcacgc ggggcgatct
      121 ccgcggtgaa cgaagcggcg agctcaattg ttggagccgg ctccccgttg tccggggatc
      181 acggctcaga ggcagcacac cagaagcggc gcggccagca gggggaggtt cttcagcggc
      241 ggggagactg ggaggttcca aggcaagggt ccagcgccgc aaccatagcg agatcgtttc
      301 caagtgaaag tgttttgttt gccggacctc gagatcgtcg cggcgaccct cgccgtgccc
      361 ccgtgtcgag accgaccggc gaccgggggc agccgacgac gctgcccgca gtcggcgccg
      421 agttttccac aggcgacgac gacgggagcc ggcgacgagc gtcggcgcga gccggcgaga
      481 gccggcggga gccggcgacg acagccggcg acgacagccg gcgggagccg gcgacgacag
      541 ccggcgacga cagccggcga cgacagtcgg cgacaacagc cggagacgat cgctaacacg
      601 cgttccggcg cgaggcgtca ggcggcgggg taacgctcga gcaagggccc gtcgtcacgt
      661 acggccacgt gggtgaccgt ctggccctgg tagcagatcg caccgcgggc gaggcccatc
      721 acccagccgt cggcctcggc ccgcagctcg ccgtgctcgg cgagcttgcg accgtgcagg
      781 tcggtcagcg tcgccagcaa gtcgcccgcc cgaaacacgt cgcccggttg cacgtgataa
      841 cgcaggaccc cgctggcccg cgcgcgaggg gtgctctcgc gcatgacggg gaagtcgacc
      901 gccggttgcg gcaccgagcg taccggctcc ggttcgccgg gcagcatgcc cgagcccttg
      961 aggatgttga ggacggcggt cgtgccagcc gcgagggcgg cggggtcgat cccgccgatc
     1021 agccccagct cgaccgtgat cgccgggatc cgggcgaggt tgagggcggc tccgctggtc
     1081 gagcggtgca ggagcttctc gacgtagcgc cccgccgggt gctcgaggac caccggcagg
     1141 ccgctggcgc ggcacagggc gtccagccga gtcagcagcg agccgagcgc gggcagctcg
     1201 gcctcgtcgc tataaaggat gcggtcgcgg atgatgaagc tggcggcctg gagcccgaag
     1261 cagtgcaggt cgacgtagag gtcggcgctg gcgcggagct cggcgaacag cgcggcgtag
     1321 ccggcctcga acacgccggg ggtctcggcc aggcgggcgc gttcggccgg gtcgccgccg
     1381 ggcgggtcga tgcccgggaa caggcggttg gggtcgcgcg cgtcgaggta gggctcgcgc
     1441 tggcgcgtgt gcaggccggc ggggttgagg ctcgggatgg cgacgatcgt gccgcggaga
     1501 tcgtcgagca gcgcgggcgt gacgacgtcg tggatgacgc ccatgccggt cagctcgtcg
     1561 ccgtggatgt tcgcggtcag ccacagcgtc ggccccggct cgcggccgcg ggcgagcagg
     1621 accgggaggc gctcgacgtg accggtcgga agatcggcga gcgtgtacca gccataagtg
     1681 agctcgccgg gggacgcggt ggctgttccg agggacaggg gccgatgcgc cgacatgcgg
     1741 cgcagtgtgc ccgatcctgc gcgccgcgac cactcacccg ggctcgagca cgaaggggta
     1801 ggtgatgatg acgctggcgc cgccgtccgg cttggggaag gtccaggtct tcacggccgt
     1861 ggcgacgcag gtgccgaccg cggcgtcggc catcgtgctc tcactgacct cggccttgag
     1921 gaccttgccg tcggcggcga ccgtgaaggt gatagcgacg cgccccttgg cgttggggtc
     1981 gcgggcgagc gcctgattgt agcaatagcg gacctcgttg atgtgggcgc gcacgatccg
     2041 gcggatgatc tccttgtcga gcgagccctg cacctcggcc ttggcctggc gcacggtcgg
     2101 gacccgcgtg ccacgcccgc cgaagccggc gccgtagccc cgaccataac cgctgccgct
     2161 gccaccaccg ccgcccctgc cgatcaggcc ggtgttgccg aggccgatcg tgccgtacga
     2221 ctcaccgatc tcctcgacgc cgagcggcag ctcgagcggg acatgcaccg tcttgagcgt
     2281 gccgtcgctc tgcttctcct gtttggtctc gaccgcgacg aagctggtgt actgcgtcat
     2341 cacgcggtgc tcgatcgcca gcttcgtcac cgcggcgacc gtcttctcgt cggcgctcga
     2401 ctgctggctg tcggggtagt tgagcagcgc gtcgaccttc tgccgggccc acatcgagcg
     2461 caggccggtg tgctcggcgc cgtcggcgaa gtcgagcctc agcggcatct cgaccggcgc
     2521 gccgccgagc ttgcccttga gcttggcctg gcccttcgcc tcgccggtga agcggccgta
     2581 gacgaccacc ggctggccgc taaacaggtc gggcagcgcc gcgggcacga cgtcggcgac
     2641 gggcaggccg ccccagtcga tctcgatgtc ggtcagcacg gggcggtcga tgtagctgta
     2701 gaagcgctcg acgacggcgt cgaccttctc gccggggccg acgtacgtga cgctgccgcg
     2761 gcccatgcgg ctgatgccct cgagcaggta gtgattgacc gagctgccga cgccgagccc
     2821 gaacaggcgc gcgtcgccgc ggacctcgcc gatcctgcgg aagatctcgg tctcgttgcc
     2881 gatgtaaccg tcggtcagga acatgaccat gcgcaggcgc tcggggtcct tcttgaaggc
     2941 gagcgcggcg gtgatgcccg cgagcatctc ggtgccgccg tcgccgctga agttgcgcat
     3001 gtactcgatg ccgcgccggc ggttctcggc ggtgttccgc agcggcgccg gggcgagctg
     3061 accggtgtcg ccggcgaagc gcaggatctg gaaggtgtcg tccgcgccca tgccgtcgag
     3121 cgccttttgc accgcggcga cggaggtgtc gagcggctcg ccctgcatcg agcccgaggt
     3181 gtcgagcgcg aacaccagct cgcgcgggcg cgccttctcc tgcgagaagc cctgcggcgg
     3241 ctcgacggcg agggtgaagt agccctgacc gtcggctgtc ttttgggcca tgaggcgcgc
     3301 gcgcggctcg aggccggcga gctgccacga gatcgagaag tcgcggttgg gccgcgcggg
     3361 cccgcgagcg agctcgaggc gggtgatgtc gccggcctgc gcgacggtca gcgagtggta
     3421 cttcgagcgc agcgcggaga ccgggaagcc ggcctcgatc gtgacctgga tgtcgaccgg
     3481 cgagcagctg acgacgtcct tgccgagctg cggcgagttg atcttcgcgg cgtcggggac
     3541 ctgcgtggtg ttgccggcgg cgtcgaccgg ggtgccgggg gcgtagcgcg ggccgacggt
     3601 gatcggcagc gtcaggccgt agcggccggc ctcgcggcgc agcggctgga cgacgtgcat
     3661 ctcgacctcg acggtctcgc cgggctggat gttggcgacc gcctgggcga acacgttggg
     3721 ccggttctgc tcgagcagcg cggcggtctg gccggcgtcg cgggccgcct cgtaggtcct
     3781 ccgcgcgtcg tcgcggctct tcatcaggcc gcggatctcg cgggtgccga cgcggatcca
     3841 gt
//
LOCUS       DASWNJ010000602         3115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_81522, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000602 DASWNJ010000000
VERSION     DASWNJ010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81522"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="VGB85_10075"
     CDS             complement(<1..1034)
                     /locus_tag="VGB85_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134419.1"
                     /translation="MSSLPTADERVWLVDQLVDLMASAGWEPLVNAPLVEPDARFFPD
                     RWSADAGGVRRMARRLLRYVGLDTLDVQVDIFDGGRTQESDGHGRSSERHEGAAAWFA
                     GIEDGVCLFGAEAGGFEDPAVLAGTMAHEVAHVYRRVRGLEVDDRDLEERLTDLTTIF
                     LGFGVLTTNASLKHRSHGDLNYHAWSRKSVGYLPPQAMSFLLALQVCARELDAAGRRH
                     VAGLLETNQRAYFAAACTWIAAQLPTLGERLGLPDPSSWPDPPALGPLWAPIEEGPEA
                     LVAAPVKPVKPVGENAGRAVFRVHHTQAATFGKAAAVLGAIVGVFGSTGAAAVWWWGG
                     AAAFVGLAVLA"
     gene            1078..1890
                     /locus_tag="VGB85_10080"
     CDS             1078..1890
                     /locus_tag="VGB85_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY0134420.1"
                     /translation="MRSLLLLTALAACRPSAAPEPAAPASPAASPVPSDSPAAAPAGA
                     SIAEILADPARPEEERALDAGRKPAELLAFLDLRPGMKAADIGAGHGYTTFLLARSVA
                     PDGRVYAQNPRFARERFTEPGFTERMKTPAFANVVRVDQEFDAPIPADVRDLDLVINF
                     LFYHDTYWMKVDNAAMNRAIFAALRPGGAYVIVDHSAAPGTGTSASQTLHRVEESVVR
                     AEVEAAGFRLAASADFLRNPADPRDWNAMPTAAAKAGRRGQADRFVLKFVKP"
     gene            1918..2553
                     /locus_tag="VGB85_10085"
     CDS             1918..2553
                     /locus_tag="VGB85_10085"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0134421.1"
                     /translation="MPAPAWIISQLGHPRGLFGRVLGRILNRANLPLNLHMVGALDLP
                     PGAEVLEVGFGGGVALELILRHAPDARVTGVERAADMLALVRRRFADALRGGRLELVD
                     ASVEQLPLADDRFDAACSANCVYFWKDVDAGLRELARVLRPGGVLVLAINSPEVLIAV
                     GFAAQGLNTLSAEELATRMRGAGFGDAYARRMPDPGRHGTALVIGHRDAAD"
     gene            complement(2767..>3115)
                     /locus_tag="VGB85_10090"
     CDS             complement(2767..>3115)
                     /locus_tag="VGB85_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134422.1"
                     /translation="ALLRLFRARQPGGDASFMTPTLLAALVEAPVAFDEHEDILRWAF
                     ATLPPAERARWAASLAAASENIGLETTGGWLAEASMCVADDQEFRRLVERAYVDFGLD
                     AEDATRAVEALGR"
BASE COUNT          435 a         1279 c          994 g          407 t
ORIGIN      
        1 gcgagcaccg cgagaccgac gaacgccgcc gcgccgcccc accaccacac cgccgccgcg
       61 ccggtcgacc cgaacacgcc gacgatcgca ccgagcaccg ccgcggcctt cccgaaggtc
      121 gcggcctgcg tgtgatgcac gcgaaacacc gcccgccccg cgttctcccc gaccggcttc
      181 accggcttca ccggcgccgc gacgagcgcc tccggcccct cctcgatcgg cgcccacaac
      241 ggccccagcg ccggaggatc cggccacgac gacggatccg gcagaccgag ccgctcgccg
      301 agcgtcggca gctgcgccgc gatccacgta caggccgccg cgaaatacgc gcgttgattg
      361 gtctcgagca gccccgccac gtggcgccgt cccgccgcgt cgagctcacg cgcgcagacc
      421 tgcagcgcca gcaggaagct catcgcctgc ggcggcaggt agccgaccga cttgcgcgac
      481 cacgcgtgat aattgagatc gccgtgggac cggtgcttga gcgacgcgtt ggtcgtcagc
      541 accccgaagc cgaggaagat cgtcgtcagg tcggtcagcc gctcctcgag gtcgcggtcg
      601 tcgacctcga gcccgcgcac gcggcgatac acgtgcgcga cctcgtgggc catcgtgccc
      661 gccagcaccg ccggatcctc gaagccgccg gcctccgccc cgaacaggca caccccgtcc
      721 tcgatccccg cgaaccacgc cgccgcgccc tcgtgccgct ccgagctgcg accgtggccg
      781 tcggactcct gcgtgcgccc cccgtcgaag atgtcgacct ggacatcgag cgtatcgagc
      841 ccgacgtagc gcagcagtcg acgcgccatg cgacgcaccc cgcccgcgtc ggcgctccag
      901 cggtcgggga agaagcgggc gtccggctcg accagcggcg cgttcaccag cggctcccag
      961 cccgccgacg ccatcaggtc cacgagctgg tcgaccagcc acacccgctc gtccgccgtc
     1021 ggcaacgagg acacgcccga tgatagcgcg accccccggg agtgatagtg tccgggcatg
     1081 cgcagcctcc tgctcctcac cgcgctcgcc gcctgccgtc cgtccgccgc cccggagccg
     1141 gccgcccccg cgtcgccggc cgcctcacct gtcccttcgg acagccccgc agcggccccg
     1201 gcgggcgctt cgatcgcgga gatcctcgcc gaccccgcgc gtcccgagga ggagcgggcc
     1261 ctcgacgccg gccgcaagcc cgccgagctg ctcgccttcc tcgacctgcg acccggcatg
     1321 aaggccgccg acatcggcgc cggccatggt tacaccacct tcctgctcgc gcgttcggtc
     1381 gcgcccgacg gccgcgtcta cgcgcagaac cctcgcttcg cgcgcgagcg cttcaccgag
     1441 ccgggcttca ccgagcgcat gaagaccccg gcgttcgcca acgtcgtccg cgtcgatcag
     1501 gagttcgacg ccccgatccc cgcggacgtg cgcgacctcg acctggtgat caactttcta
     1561 ttttatcacg acacgtactg gatgaaggtc gacaacgccg cgatgaaccg ggccatcttc
     1621 gcggcgctgc ggcccggcgg cgcctatgtc atcgtcgatc acagcgctgc gccgggcacg
     1681 ggcaccagcg cctcgcagac gctgcatcgc gtcgaggagt cggtcgtgcg cgccgaggtc
     1741 gaggcggcgg gctttcgtct ggccgccagc gccgacttcc tgcgcaaccc ggccgaccca
     1801 cgcgactgga acgccatgcc gaccgccgct gccaaggcgg gacgtcgcgg ccaggccgac
     1861 cgcttcgtcc tcaaattcgt gaagccctga tctcctcccc tgatctagac tccgcgcgtg
     1921 cccgcccccg cctggatcat cagtcaactc ggccatccgc gcggcctgtt cggtcgcgtg
     1981 ctcggccgga tcctcaaccg cgccaacctc ccgctcaacc ttcacatggt cggcgcgctc
     2041 gacctgccgc ccggcgccga ggtgctcgag gtcggcttcg gtggtggcgt cgcgctcgag
     2101 ctgatcctcc gccacgcgcc cgacgcccgc gtcaccggcg tcgagcgggc cgccgacatg
     2161 ctcgccctcg tgcgccgccg cttcgccgac gccctgcgcg gcggccggct cgagctggtc
     2221 gacgcctcgg tcgagcagct cccgctcgcc gacgaccgct tcgacgcggc ctgctcggcc
     2281 aactgcgtct acttctggaa ggacgtcgac gccggcctgc gcgagctggc ccgcgtgctg
     2341 cgccccggcg gcgtcctggt cctcgccatc aactcgcccg aggtcctgat cgccgtcggc
     2401 ttcgcggccc agggcctcaa tactctcagc gccgaggagc tcgccacgcg catgcgcggc
     2461 gccggcttcg gcgacgcgta cgcccgccgc atgcccgacc ccggccgtca cggcaccgcg
     2521 ctggtgatcg gccaccgcga cgccgccgac tgagccgcgc aactgactcg aggtcggacc
     2581 gcacggccgt ccgaccgagg cgcacgcgca gaacctgagc gacctcgcgg cgctcgctca
     2641 tcgcgacccc gcccatctcg cggaagatcg ctcattgcaa cgcggccgac cctgcgcgac
     2701 tactcaagga tgtcgtttgg cgcgacgcgg ccgaccgaga cacaattcgg ccctgagcga
     2761 gacgcgctaa cgcccgagcg cctcgaccgc tcgcgtcgcg tcctccgcgt cgagcccgaa
     2821 gtcgacgtag gcgcgctcga ccagccgcct gaattcttgg tcgtcggcga cgcacatcga
     2881 ggcctccgcg agccagcccc cggtggtctc gagcccgatg ttctcgctgg ccgccgcgag
     2941 cgacgcggcc cagcgggcgc gctcggccgg gggcagcgtg gcgaacgccc agcgcaggat
     3001 gtcctcgtgc tcgtcgaacg cgacgggcgc ctctacgagc gcggccagca gcgtcggcgt
     3061 catgaagctc gcgtccccgc ccggctgccg cgcgcgaaac agccgcagca gggca
//
LOCUS       DASWNJ010000603        14030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_541494, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000603 DASWNJ010000000
VERSION     DASWNJ010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 14030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14030
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541494"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VGB85_10095"
     CDS             complement(<1..513)
                     /locus_tag="VGB85_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134423.1"
                     /translation="MHLTSRRTLAALLFTAAAATSTSAHADEDIKLRTTWTGNLNFFS
                     TGASLAADSDDDGEVDEIAQPGTVTVTALDVPLGSTLDTAYLYWGATQNTAKCEGDAH
                     LDKEVVFGAPGVNPIAVTAEVCYCSASTTYDMQLCRADVTALVSEMQGEYSVGELSAL
                     IENGDTNNASF"
     gene            complement(626..1558)
                     /gene="coaA"
                     /locus_tag="VGB85_10100"
     CDS             complement(626..1558)
                     /gene="coaA"
                     /locus_tag="VGB85_10100"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEY0134424.1"
                     /translation="MRRSLFLDFDHEQWGRLRADTPMTLTEADLGRIRGLNESVSMHE
                     VVQVYLPLSRLLNLHVAASQELYGATSTFLGHPESVRVPYIIGVAGSVAVGKSTTARL
                     LRELLARWPNSPRVDLVTTDGFLLPNATLEARGLMHRKGFPESYDARSLLAFVADIKA
                     GRVGVQSPVYSHESYDILPDQMITLGASDIVIIEGLNVLQTRTDPDAPQTFVSDYFDF
                     SIYVDADPDDLERWYLERFLTLQRTAFRRPRAYFNRYAHLSEHEARTIAAGIWREINA
                     RNLRENILPTRERADLILEKSDDHSIRRVRLRKR"
     gene            complement(1566..3122)
                     /locus_tag="VGB85_10105"
     CDS             complement(1566..3122)
                     /locus_tag="VGB85_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134425.1"
                     /translation="MKTSLSRAVLLGSALVLTPACRKREPPKVEIFDEAATPAQTEAL
                     GSLAPPWQAPERVTLDNGLLVHWLHEAETPAVHLRLILPTHGLKGAQQGAPALVAARA
                     LVSELEYLASRATGPEGTPRLASYVELHAGVDRLEVVLHGVAADTTTALAVFAEALAA
                     DPTRALGSARKALLGELRPVDTEVVATSELVAELRGGDAGRERIDAAVLQAIEESTLQ
                     DAWKALTDPRQALLVVHAKARLADATMTAATTDLATRWRQKVSLLPGVDGRSEPAALK
                     RLRPNKQPGSTGKHVTKAPLAPLRRIDTPGRGRAQLVIGRAIPTGSARDRSLARLAQR
                     RLQEQFDARLTIAGSWAALTLHIPLAAAGKPKPATDPLEGTDPPKDVDEAPEDPLTRR
                     LRRELTLVRGYVRLRPTTQDLFQVAQLWLGARMVAASVDGEDWTALWSDSLDLAADDG
                     EIAAALARDAQAMLAATPEQVVAWQQRWLDFSTSEPGWAWVLVGQEKDLQGVHEFLKK
                     GMSFDPPPEQ"
     gene            complement(3119..4603)
                     /locus_tag="VGB85_10110"
     CDS             complement(3119..4603)
                     /locus_tag="VGB85_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134426.1"
                     /translation="MLVALLTLLAAAPAVAPAAPAAPAAPAGGTAAGGGTAAGGGAAA
                     AGGASPSPAPTAAAPGDTTDIVVDALLPCGLRVLTARDMTLPVASVVLAIETGSEDDP
                     PDAPGLVHALAYQVLQGNRELRPGEVIAAAQDAGGLHGMATGFAQVRYESLVPATRLD
                     AVLWAESQRLRFPTTAPQRWEQTLGWAAGDERTGSRLRPEALAYVHGAPGLGHDGRAV
                     GKSLAAMVEGVLAQQMSQKFNYTRATLVVVAPESPERMLERITPFFAGLPQQRRTAPL
                     RRLGPRPAAAASPASSPASSPAPGAPAMSPGTGATVPLSRQRGHTFVWAVPPTPAANA
                     WAAAICRAINRQKKPPEAEEPRKARIGCDLESDPRRGVLIMRPSGTDDPWAMVRGRLA
                     RLAPGGPDAALLQAQAATVTQALRLTQRTPQGLAQQLAMSPAEESTSPQPAFTRQHRV
                     DDLTGVTALADPASLQREAPDLLRVEAAVVLTYPETAEAPKAAP"
     gene            complement(4673..5953)
                     /gene="eno"
                     /locus_tag="VGB85_10115"
     CDS             complement(4673..5953)
                     /gene="eno"
                     /locus_tag="VGB85_10115"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEY0134427.1"
                     /translation="MSIIVHVQAREILDSRGNPTIEVEVGSESGNVGRAAVPSGASTG
                     EHEAVELRDGDKARYHGKGVSKAVEHVNDTLAEVVLGFDVRDQLAVDQALIDADGTAN
                     KGKLGANAILGVSLAVARAAALDTDLSLYRYLGGPLANLLPVPLMNVINGGAHADNNL
                     DFQETMLVPHGFDTFSDALRAGVEIFHTLKGILRKQKKTTAVGDEGGFAPDMANNAEA
                     LATLLQAIEQAGYQPGKQVSLALDVAASEFFDKQKGYVLAGEGGRVLDSAALVDLYAE
                     LASKFPLVSIEDGMAEDDWDGWKALTDRLGATHQLVGDDLFVTNPERLRRGIERGIAN
                     SILVKVNQIGSLSETLEAVRMAQHRGYRAVISHRSGETADTFIADLAVATAAGQIKTG
                     SASRSDRVEKYNQLLRIEEALGEHARFAGKELFK"
     gene            complement(6029..7549)
                     /locus_tag="VGB85_10120"
     CDS             complement(6029..7549)
                     /locus_tag="VGB85_10120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY0134428.1"
                     /translation="MTPGPTPAALERRALALISVLPLVVHLLVGEGYGYFRDELYYLA
                     CAQHLDLGYVDHPPLIVWIVAVVRATLGDSLLALRLVPALAHAGTVWTTAAIAAQLGG
                     RRFAQILAALAALLAPVYLSLASVLSMNALDVLLWAVAGLVAIRALRGDSRQWLRFGV
                     VAGLGLLNKLSFVFLGVGLVVGLVLARRDVFKDRWLWRGAAIAAAIALPHLIWQVMHG
                     WPTLEFMANARAHKNVALGPLAFLQQQVLQIGPAALLLWVPGLVWLLAAGAARPYRPL
                     GWAYLAILAMMLGTAAKPYYLAPAYTPLLAAGAVVVSAKVSRRGLRALLVLVLVVCGL
                     VAMPLARPVLAVESFVAYADALGMTPKSDERQPLGRLPQFFADMHGWPEFAATVAIAF
                     KNLPGEEQAVACVVGRNYGEAGAIDFFGKALGLPRAISTHNNYFLWGPRDCSGEVVLM
                     PAKDPDDLREVFSRVEPGAVVDCRDCMPYEDDLTIWVAREPRRPIAGLWPQLKHYE"
     gene            7621..8676
                     /locus_tag="VGB85_10125"
     CDS             7621..8676
                     /locus_tag="VGB85_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEY0134429.1"
                     /translation="MIKYLGSKRTLVPRLCELVQALGPRSVLDLFSGTSRVGHALKRA
                     GLQVHANDHNAYAATLARCYVQADRDELLADATRLITELNALPPAAGYFTETFCERSR
                     FFQPKNGARVDAIREAIAAKGLEPELEAILLVSLMEAADRVDSTTGVQMAYLKQWAAR
                     AHNILELRLPDVLPRAPHGKGHAHQLDAMLAATQLQADLAYLDPPYNQHKYLGNYHIW
                     ESLVLWDKPETYGTACKRVDCRVRTSPFNSRVDSAAAMRTLISSVQARHLVVSFSDEG
                     HIGRDEMEAMLRARGEVLVLAHDFKRYVGAQIGIYNPRGEKVGSAGHLRNTEYIYIVS
                     DSPEALRRIPQDVAATC"
     gene            8670..9299
                     /locus_tag="VGB85_10130"
     CDS             8670..9299
                     /locus_tag="VGB85_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134430.1"
                     /translation="MLTIDPHPLLDCCAFVTTFPRPLGELPSPLRLLAGLALDYADAP
                     IHSDDSIRAAVRDLLRHGGLFKPTGRSKPASEYLLKAASEGMDSINLAVDACNVASLH
                     SGLPISVVDLDLVTPPLRVAVAPAGTSYVFNKSGQEIAIGGLLSLWDAEGPCAGPVKD
                     SQRSKTHAGTLRTLSIVWGTIGAPGRAWQTTAWYRELLHTAGATTEAAP"
     gene            complement(9305..10510)
                     /locus_tag="VGB85_10135"
     CDS             complement(9305..10510)
                     /locus_tag="VGB85_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134431.1"
                     /translation="MQNNRSHDSYARLTCLALLLGACNSGGSTTEGTDSATGTSGSTT
                     GAPTTSTSETATTDTATDSPTGPGSESATGSTTGSTTDESTTSTTSTTSSTSSTTDTT
                     QGVDSTTDEPGTTGTGTTGAGTTGDPSSSGGESDTTDSSSTTGNADCVPTEQVEVSCD
                     LIDNDCDDSIDNVDAGMDGICDCLTIGILGDSGFAPNANFEAWLEEQGSAVTRTKLAN
                     NPGVVTPQFLAQYDLVLVDRIERQLSADEAAALSTFVKNDGRGMITLIGYNFDNGNPA
                     PERDRANTALAPFGLAYQGGYFGDGVIPTFDQGHPISKGIFDVNFAGGIMPVDNGKQG
                     ISEVFATWNAMTAGLAHQTAMEGGRVIVWGDEWLTFDSDWQGYADVQDLWVNMVSWAK
                     PKDFCGVPQ"
     gene            complement(10539..12458)
                     /locus_tag="VGB85_10140"
     CDS             complement(10539..12458)
                     /locus_tag="VGB85_10140"
                     /inference="COORDINATES: protein motif:HMM:NF036933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HEY0134432.1"
                     /translation="MSTLSPRLPLVLLLAAPLACSDDGNTSTTTAGTTGTSSATVDPT
                     AGVTTSGTVTEGTTPTTGEMTTQSSMGGTNSTTSEPTTDPATDTAVSGTTTTGEGTTT
                     TTATTETTTGDTTTTAAESSSGKVCVCDPGSTNGCDMGDLLTCTDDCEGFAPVPCPPG
                     QACFNGACQAKLCEPNMVVCEGTNQTKQCNAQGDGFDPPVMCGMTQECSLGACTELCE
                     LVKSNPSSVGCVFRGNKMQNFQEEPSAVTVGNVSKTLTASVTMYFFEGGQEKVVTGPL
                     SVAPGKSAQFDLLNPPQPGIGSVLRPDSTFKIVSTVPLVAYLHSPIKAMAHNDSSMLL
                     PDHAQQKNYIIPAYTPNVGGNPAYFNVIGLKDGTVVSWTPPVATQAGNGVPAVAANQT
                     GMVTVGDYDLLQISSGQSDLSGTIVSLSEPAWVVGAVQCVNVPANVTFCDHIEEQAIP
                     LEYWGKVYVGAHAPKRGTENYWWRVYSGEDNTTIETIPPQPGFPITLNKGKFHEFKTK
                     DSFIFSGSGPIMPVQYLEGQNGGAGTGDPASYQMVPVEQFLDRYVFVTGIGYNLHFVQ
                     VVRDKGGPDILVDGVPVTGYYAVGEYEVADWKISEGTHLAESDASFGIYQVGYTGVTS
                     YAYPGGLRLKVINPQ"
     gene            12595..13464
                     /locus_tag="VGB85_10145"
     CDS             12595..13464
                     /locus_tag="VGB85_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134433.1"
                     /translation="MRPGQLSWTLLVMACAAAPAPTVVPVAVKEVVPVVTPAAEPEPV
                     PAGLDIAVACADMDPAAPVRVRIEDAVLECPEGRLESCRGETRFFFANCGDEPVTLRG
                     VELIDHGSHGRVMYDFDNREIAASGIWPWTRKVYFDGASQLKVDVVDSHGAPITVAEK
                     PVRTSHPQRDAAIAACVACNGVWGVHGMLPREGCNCQARDAFKNCHDGDECEGACLFD
                     QWQISQPAQRGGCTGTGKQTVCTARVRAIGRPVGRCSGFVGVRSCHNLLQRGIASEPD
                     REIPWGTRRVCSD"
     gene            13561..>14030
                     /locus_tag="VGB85_10150"
     CDS             13561..>14030
                     /locus_tag="VGB85_10150"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY0134434.1"
                     /translation="MSGHETILGHLATWGLSQRYEDTATEGLAFLLRRDPTLRKQFVA
                     LLQKAQPGLPDNLRFTTQETFDGCRIDMCGRTDSGAPVFIENKFWAALTGNQPVAYLE
                     RLARQQVPSLLLFIVPERRRASLWPELLSRLTAGGVAYQAAGAHRVEIVGTQHS"
BASE COUNT         2017 a         4867 c         4967 g         2179 t
ORIGIN      
        1 gaacgaggca ttgttggtat caccattctc gatcagcgcc gagagctcgc cgaccgagta
       61 ttcgccctgc atctcgctga cgagcgcggt gacgtcggcg cgacacagct gcatgtcgta
      121 ggtcgtcgac gccgagcagt agcagacctc ggcggtgacc gcgatcgggt tgacgcccgg
      181 cgccccgaac acgacctcct tgtcgaggtg cgcatccccc tcacacttcg cggtgttctg
      241 ggtggccccc cagtacaggt acgcggtgtc gagggtcgag ccgagcggca cgtccagcgc
      301 ggtgaccgtc accgtgccgg gctgcgcgat ctcgtcgacc tcgccgtcgt cgtcggagtc
      361 ggccgccagc gaggcgcccg tgctgaagaa gttcaggttg cccgtccacg tggtccgcag
      421 cttgatgtcc tcgtcagcgt gggcgctggt cgaggtcgcg gcggcggcgg tgaacaggag
      481 ggcagcgagg gttcggcggc ttgtcaggtg cataagcatt ggtccagttc gcccaagcat
      541 ggcctgcctg ggcggatctg gcccgtcgcc gggtgatcgg gcgcaccgcc ccgtgctcgg
      601 agatccggac cggggtttcg agacctcacc gcttgcgcag ccggaccctg cggatcgaat
      661 ggtcgtccga cttctcgagg atcaggtcgg cgcgctcgcg ggtggggagg atattttccc
      721 gcaggttcct ggcgttgatc tcgcgccaga tcccggcggc gatcgtgcgg gcctcgtgct
      781 ccgagaggtg cgcatagcga ttgaagtatg cgcgcggccg gcgaaacgcg gtccgctgta
      841 acgtcaggaa gcgctcgaga taccagcgct cgaggtcgtc cgggtcggcg tcgacgtaga
      901 tcgaaaagtc gaagtagtcc gagacaaagg tctgcggcgc gtcggggtcg gtgcgcgtct
      961 gcaggacgtt gaggccctcg atgatgacga tgtccgaggc cccgagcgtg atcatctggt
     1021 ccgggaggat gtcgtagctc tcgtgcgaat ataccgggct ctgcacgccg acccggcccg
     1081 ccttgatgtc ggcgacgaag gccaggagac ttcgcgcgtc gtagctctcc gggaagccct
     1141 tgcggtgcat gaggccccgc gcctcgagcg tcgcgttggg cagcaagaag ccgtcggtgg
     1201 tgacgaggtc gacgcgcggg ctgttgggcc agcgggccag cagctcgcgc agcaggcggg
     1261 cggtggtgct cttgccgacc gcgacgctgc cggcgacgcc gatgatgtac ggcacgcgca
     1321 cggactcggg gtggccgagg aaggtggagg tcgcgccata cagctcctgg ctggcggcga
     1381 cgtggaggtt gagcaggcgc gagagcggga ggtagacctg cacgacctcg tgcatcgaca
     1441 ccgactcgtt gaggccgcgg atccgcccga ggtcggcctc ggtgagcgtc atcggagtgt
     1501 cggcgcgcag ccgtccccat tgctcgtggt cgaagtcgag aaatagggag cggcgcatcg
     1561 cgctttcact gctcgggcgg cggatcgaag ctcatgccct tcttgaggaa ctcgtgcacg
     1621 ccctgcaggt ccttctcctg gccgacgagc acccaggccc acccgggctc gctggtgctg
     1681 aagtcgagcc agcgctgctg ccaggcgacg acctgctccg gggtcgcggc gagcatcgcc
     1741 tgcgcgtcac gagccagcgc cgctgcgatc tcgccgtcgt cggcggcgag gtcgagcgag
     1801 tcgctccaca gcgcggtcca gtcctcgccg tcgacgctcg cggcgaccat gcgcgcgccg
     1861 agccacagct gcgcgacctg gaacaggtcc tgcgtggtcg gccgcaggcg gacataaccg
     1921 cgcaccagcg tcagctcgcg tcgcagcctg cgggtcagcg ggtcctccgg cgcctcgtcg
     1981 acatccttgg gcgggtcggt gccctcgagc gggtcggtcg cgggcttggg cttgccggcc
     2041 gcggcgagcg ggatgtgcag cgtcagcgcg gcccacgacc ctgcgatcgt caggcgggcg
     2101 tcgaactgct cctgcagccg gcgctgggcc aggcgcgcga ggctgcggtc cctggcgctg
     2161 ccggtcggga tcgcccggcc gatcaccagc tgcgcgcgcc cgcggcccgg cgtgtcgatc
     2221 cggcgcagcg gcgcgagcgg ggccttggtg acgtgcttgc cggtgctgcc cggttgcttg
     2281 ttcggccgca gacgcttgag cgccgccggc tccgagcgac cgtcgacgcc cggcaacagc
     2341 gagaccttct gccgccagcg cgtcgccagg tcggtggtcg cggcggtcat cgtggcgtcc
     2401 gcgaggcgag ccttcgcgtg cacgaccaac agcgcctggc gcgggtcggt cagggccttc
     2461 cacgcgtcct gtagcgtgct ctcttcgata gcctgcaaca ccgcggcgtc gatgcgctcg
     2521 cgcccggcgt cgccgcctcg cagctcggcg acgagctcgc tggtcgcgac gacctcggtg
     2581 tcgaccggac gcagctcgcc gagcagggcc ttgcgcgcgc tgcccagcgc gcgggtcggg
     2641 tcggcggcga gcgcctcggc gaacacggcg agcgcggtgg tcgtgtcggc cgcgacgccg
     2701 tgcagcacga cctcgaggcg gtcgacgccg gcgtgcagct cgacgtagct ggcgagccgc
     2761 ggcgtgccct cggggccggt cgcgcgcgag gcgaggtact cgagctcgga gaccagcgcg
     2821 cgcgctgcga ccagcgccgg tgcgccctgc tgcgccccct tgagcccgtg ggtcggcaag
     2881 atcaggcgca ggtgcaccgc gggggtctcg gcctcgtgca gccagtggac cagcaggccg
     2941 ttgtcgagcg tcacgcgctc gggcgcctgc cacggcggcg cgagcgaccc gagcgcctcg
     3001 gtctgggccg gcgtcgccgc ctcgtcgaag atctcgacct tgggcggctc gcgcttgcga
     3061 cacgccggcg tgagcaccag cgccgaaccg agcagcaccg cgcgtgagag cgatgtcttc
     3121 atggggccgc cttcggcgcc tcggccgtct cggggtacgt caacacgacc gccgcctcga
     3181 cgcgcagcag gtccggcgcc tcgcgttgca gcgacgcggg gtcggcgagc gcggtgacgc
     3241 cggtgaggtc gtcgacgcgg tgctggcggg tgaacgccgg ctgcgggctg gtgctctcct
     3301 cggcggggct catcgccagc tgctgcgcga ggccctgcgg ggtgcgctgg gtcaggcgca
     3361 gcgcctgggt gacggtggcg gcctgggcct gcagcagcgc ggcgtcgggg cctccggggg
     3421 cgagccgcgc gagccggccg cggaccatcg cccacggatc gtcggtgccg ctcgggcgca
     3481 tgatcagcac gccgcgccgc gggtcggact cgaggtcgca gccgatcctc gccttgcgcg
     3541 gctcctcggc ctcgggcggc ttcttctggc ggttgatcgc gcggcagatc gctgcggccc
     3601 acgcgttcgc ggccggggtc ggcggcaccg cccacacgaa ggtgtgcccg cgctggcgcg
     3661 acagcggcac cgtcgcacct gtccctgggg acatggccgg agcgccaggt gcgggcgatg
     3721 atgcaggtga tgaagcaggc gacgcagcag cggcggggcg cgggccgagg cggcgtagcg
     3781 gggcggtgcg gcgctgctgc ggcaggccgg cgaagaacgg ggtgatgcgc tcgagcatgc
     3841 gctcgggcga ctcgggcgcg acgaccacca gcgtcgcccg cgtataattg aacttctggg
     3901 acatctgctg ggcgagcacg ccctcgacca tcgccgccag cgacttgccg accgcgcggc
     3961 cgtcgtggcc gaggccgggc gcgccgtgca cgtaggcgag cgcctcgggt cgcagccgcg
     4021 agcccgtgcg ctcgtcgccc gcggcccagc cgagcgtctg ttcccagcgc tgcggcgcgg
     4081 tcgtcgggaa gcgcaggcgc tgcgactcgg cccacagcac ggcgtcgagg cgggtcgccg
     4141 ggaccaggct ctcgtagcgg acctgcgcga agccggtcgc catcccgtgc agcccgccgg
     4201 cgtcctgcgc cgctgcgatc acctcgcccg ggcgcagctc gcggttgcct tgcagcacct
     4261 gatacgcgag cgcgtggacc agcccgggcg cgtcgggcgg gtcgtcctcg ctgccggtct
     4321 cgatcgcgag caccaccgac gcgaccggca gggtcatgtc gcgcgcggtc aacacccgca
     4381 gcccgcaggg cagcagcgcg tctacgacga tgtcggtcgt gtcaccggga gcggccgccg
     4441 tcggagccgg gctcggcgag gcgccgccag cagcagcagc tccaccgccc gcagcagttc
     4501 cgccgcccgc agcagttccg ccagcgggag cagccggcgc tgcgggagcg gccggtgcga
     4561 ccgcgggagc ggcggcgagg agggtgagga gggcgacgag catggcagct ggtgcgtggc
     4621 aggtgcttag ccgcaagcgg gcgccgcggc aagcgacgcg cccgcggtcc gactacttga
     4681 acagctcctt gccggcgaag cgggcgtgct cgccgagggc ctcctcgatg cgcagcagct
     4741 gattatactt ctcgacccga tccgagcgcg aagccgagcc ggtcttgatc tggccggccg
     4801 cggtggcgac cgcgaggtcg gcgatgaagg tgtcggccgt ctcgccgctg cggtgcgaga
     4861 tgaccgcacg gtaaccgcgg tgctgggcca tgcgcacggc ctcgagcgtc tcgctgagcg
     4921 agccgatctg gttgaccttc accaaaatcg agttggcgat gccgcgctcg atgccgcgac
     4981 gcaggcgctc cgggttggtg acgaacaggt cgtcgccgac gagctgatgc gtcgcgccga
     5041 ggcggtccgt cagcgccttc cagccgtccc agtcgtcctc ggccatgccg tcctcgatcg
     5101 agaccagcgg gaacttgctc gccagctcgg cgtacaggtc gaccagcgcc gccgagtcga
     5161 gcacgcgccc gccctccccc gcgagcacgt agcccttttg cttgtcaaaa aactcggagg
     5221 ccgcgacgtc gagcgccagg ctgacctgct tgcccggctg atacccggcc tgctcgatcg
     5281 cctgcagcaa ggtcgccagc gcctcggcgt tgttcgccat gtccggcgcg aagccaccct
     5341 cgtcgccgac cgcggttgtc ttcttctgct tgcgcaggat gcccttgagc gtatgaaaga
     5401 tctcgacccc ggcgcgcagc gcatcgctga acgtgtcgaa gccgtgcggc accaacatcg
     5461 tctcctggaa gtcgaggttg ttgtccgcgt gcgctccacc gttgatgacg ttcatcagcg
     5521 gcaccggcag caggttcgcc agcggcccgc cgaggtagcg atacagcgag aggtccgtgt
     5581 cgagcgccgc cgcgcgggcg accgccagcg acacgccgag gatcgcgttg gccccgagct
     5641 tgcccttgtt cgccgtgccg tccgcgtcga tcaacgcctg gtcgaccgcc agctggtcac
     5701 gcacgtcgaa gccgagcacg acctcggcga gcgtgtcgtt gacgtgctcg accgccttgg
     5761 acacgccctt gccgtggtag cgcgccttgt cgccgtcgcg cagctcgacg gcctcgtgct
     5821 cgccggtgct ggcgcccgag ggcaccgcgg cccgcccgac attgccggat tcgctgccga
     5881 cctcgacctc gatcgtcggg ttgccccgcg agtcgaggat ctcccgcgcc tgcacgtgaa
     5941 cgatgatgct catggcccgg gatggtaacg cggacgcccc gctccgcacg tccacaattg
     6001 cgtgcgtttt cgcgccgcga ggctgccgtt actcataatg cttgagctgc ggccacagcc
     6061 cggcgatcgg cctccggggc tcacgggcga cccagatcgt caggtcgtcc tcgtagggca
     6121 tgcagtcgcg gcagtcgacc acagcccccg gctcgacgcg ggagaacacc tcccgcagat
     6181 cgtccgggtc cttcgcgggc atcagcacga cctccccgct gcaatctcgc ggcccccaca
     6241 ggaagtaatt gttgtgcgtg gagatcgccc gcggcagccc gagcgctttt ccaaaaaaat
     6301 cgatcgcgcc ggcctccccg tagttgcgac cgaccacgca ggcgaccgcc tgctcctcgc
     6361 ccggcaaatt tttaaaagct atcgccacgg tcgccgcgaa ttcgggccag ccgtgcatgt
     6421 cggcgaagaa ctgcggcaac cgaccgagag gctgccgctc gtcgctcttc ggcgtcatgc
     6481 cgagcgcgtc cgcgtaggcg acgaagctct cgaccgcgag cacgggccgc gcgagcggca
     6541 tggcgacgag cccacacacc acgagcacca gcacgagcag cgcgcgcaga ccacgacgcg
     6601 agaccttcgc gctgacgacc accgcgccgg ccgcgagcag cggggtgtat gcgggcgcga
     6661 ggtaatacgg cttggccgcc gtgccgagca tcatcgccag gatcgccagg taggcccagc
     6721 cgagcgggcg atacggccgc gccgcgccgg cggcgagcag ccacaccagc cccggcaccc
     6781 acagcagcag cgcggccggc ccgatctgca gcacctgctg ttgtaagaaa gccagcggcc
     6841 cgagcgcgac gttcttgtgc gcgcgcgcgt tggccatgaa ctcgagcgtc ggccagccgt
     6901 gcatcacctg ccagatcagg tgcggcagcg cgatcgctgc cgcgatcgcg gcgccgcgcc
     6961 acagccacct gtctttaaag acatcccgac gcgcgagcac gaggccgacg accaggccga
     7021 ccccgaggaa cacgaagctc aatttattca gcaggccgag gcctgcgacc acgccgaagc
     7081 gcagccattg tcgcgagtca ccgcgcagcg cccggatcgc cacgaggccg gcgacggccc
     7141 acagcagcac gtcgagggcg ttcatcgaca gcacgcttgc gagcgagagg tacaccgggg
     7201 ccagcagggc cgccagcgcc gcgaggatct gggcgaagcg ccggccgccc agctgcgccg
     7261 cgatcgcagc ggtcgtccac accgtgcccg cgtgggcgag cgccggcacc aggcgcagcg
     7321 ccagcagcga gtcgccgagg gtcgcgcgca cgacggcgac gatccacacg atcaacggcg
     7381 ggtggtcgac gtagccgagg tcgaggtgct gcgcgcaggc gaggtagtac agctcgtcgc
     7441 ggaagtagcc gtagccctcg ccgaccagca ggtgcacgac cagcggcagg acggagatca
     7501 gggcgagggc gcggcgctcc agcgccgccg gtgtgggacc gggggtcatg ccgcgtttgt
     7561 acaccgcccc cgccgtcttg acgcgcgcga cacgaacctc caccctgggc ccgcgccgcg
     7621 atgatcaagt acctcggctc caagcggacg ctggtcccgc gcctgtgtga gctcgtccag
     7681 gccctcggcc cgcgctcggt gctcgacctg ttctccggca cctcccgcgt cggccacgcg
     7741 ctcaagcggg ccggcctgca ggtccacgcc aacgaccaca acgcctacgc ggcgacgctc
     7801 gcccgctgct acgtgcaggc cgaccgcgac gagctgctcg ccgacgcgac acgtctaata
     7861 accgagctca acgccctccc gcccgcggcc ggctacttca ccgagacctt ttgcgagcgc
     7921 agccgcttct tccagccgaa aaacggcgcc cgcgtcgacg cgatccgcga ggcgatcgcc
     7981 gccaagggcc tcgagcccga gctcgaggcc attttattag tatcgctgat ggaggccgcc
     8041 gaccgcgtcg attcgaccac cggcgtgcag atggcatatc tcaagcagtg ggccgcccgc
     8101 gcccacaaca tcctcgagct gcgcctgccc gatgtcttac cgcgcgcgcc ccacggcaag
     8161 ggccacgcgc atcagctcga cgcgatgctc gccgccacgc aattacaggc cgacctcgcc
     8221 tacctcgacc ctccctataa tcagcacaag tacctcggca actatcacat ctgggagtcg
     8281 ctggtcctgt gggacaagcc cgagacctac gggaccgcct gcaagcgggt cgactgccgg
     8341 gtgcgcacga gcccgttcaa ttcgcgggtc gacagcgccg cggcgatgcg cacgttgatc
     8401 tcgagcgtgc aggcgcgcca cctggtcgtc tcgttcagcg acgagggtca catcggccgc
     8461 gacgagatgg aggcgatgct gcgcgcgcgc ggcgaggtgc tggtgctcgc ccacgacttc
     8521 aagcgctacg tcggcgccca gatcggaatc tataatccgc gcggcgagaa ggtcggcagc
     8581 gccggccacc tgcgcaacac cgaatacatc tacatcgtct ccgactcccc cgaggccctg
     8641 cgccggatcc cacaggatgt cgctgccaca tgctgaccat cgacccgcac ccgctgctcg
     8701 actgctgcgc cttcgtcacc accttcccgc gcccgctcgg cgagctgccc tcacccctcc
     8761 ggctgctcgc cggcctcgcg ctcgactatg cagatgcgcc gatccacagc gacgacagca
     8821 tccgcgccgc cgtgcgcgac ctcctgcgcc acggcggcct ttttaagccg accggccgca
     8881 gcaagccggc ctcggagtat ttattaaagg ccgcgagcga ggggatggac agcatcaacc
     8941 tcgcggtgga cgcgtgcaac gtcgcgtcgc tgcacagcgg gctgccgatc agcgtcgtcg
     9001 acctcgacct cgtgaccccg ccgctgcgcg tcgccgtcgc gcccgcgggc acgagctacg
     9061 tttttaataa gtcagggcag gagatcgcga tcggcgggct gctgtcgctg tgggacgccg
     9121 aggggccgtg cgcggggccg gtcaaggact cgcagcgcag caagacccac gcggggaccc
     9181 tgcgcacatt gtcgatcgtg tggggcacga tcggcgcgcc ggggcgggcg tggcagacga
     9241 cggcgtggta ccgcgagctg ctacacacgg ccggcgcgac gaccgaggcc gctccctgag
     9301 cgactcactg cgggacgccg cagaagtcct tgggcttggc ccagctgacc atgttgaccc
     9361 acaggtcctg cacgtcggcg tagccctgcc agtcactatc gaaagtcagc cactcgtcgc
     9421 cccacacgat cacgcggccg ccctccatcg cggtctggtg ggcgaggccc gcggtcatcg
     9481 cgttccaggt cgcgaacacc tcagagatgc cctgcttgcc gttgtcgacc ggcatgatgc
     9541 cgccggcgaa gttgacgtcg aagatgccct tcgagatcgg gtggccctgg tcgaaggtcg
     9601 ggatcacgcc gtcgccgaag tagccgccct gatacgcgag gccgaacggc gcgagcgcgg
     9661 tgttggcccg gtcgcgctcg ggggcggggt taccattatc gaagttgtag ccgatcagcg
     9721 tgatcatgcc gcggccgtcg tttttgacga aggtcgacag cgcggcggcc tcgtcggcgg
     9781 acagctggcg ctcgatgcgg tcgacgagca cgaggtcgta ctgcgccagg aactgcggcg
     9841 tgaccacgcc ggggttgttg gccagcttgg tgcgcgtgac cgcggagccc tgctcctcga
     9901 gccacgcctc gaagttggcg ttcggcgcga agccggagtc gccgaggatg ccgatcgtca
     9961 ggcagtcgca gatcccgtcc atgccggcgt cgacgttgtc gatgctgtcg tcgcagtcgt
    10021 tgtcgatcag gtcgcagctg acctcgacct gctcggtcgg cacgcagtcg gcgttgccgg
    10081 tcgtgctcga gctgtccgtc gtgtccgact cgccgcccga gctgctcggg tcgcccgtcg
    10141 tgcccgctcc cgtcgtgccc gtgccggtcg tgcccggctc gtccgtcgtc gagtcgaccc
    10201 cctgcgtcgt gtcggtcgtg gatgatgtgg atgatgtggt cgaggtcgtc gaggtcgtcg
    10261 actcatcggt cgtcgaaccg gtggtcgagc cggtcgccga ctcgctgccg gggccggtcg
    10321 gtgagtccgt cgccgtgtct gtggtggcag tctcgctggt ggaggtcgtc ggcgcgccgg
    10381 tcgtcgagcc ggacgtgccg gtcgcgctgt cggtcccctc ggtggtcgag ccgccggagt
    10441 tgcaggcgcc gagtagcagc gcgaggcaag tcagacgcgc gtaggaatca tggctgcgat
    10501 tattttgcat atgtcctgtc gaattaatca ataaagcgtc actgggggtt gatgaccttg
    10561 aggcgcaggc caccggggta tgcgtacgag gtcacgccgg tgtagccgac ctgatagatc
    10621 ccgaacgacg cgtcgctctc ggcgaggtgg gtgccctcgg agatcttcca gtcggcgacc
    10681 tcgtactcgc cgaccgcgta gtagccggtc acgggcacgc cgtcgacgag gatgtcgggt
    10741 ccgcccttgt cgcggacgac ctgcacgaag tgcaggttgt agccgatgcc ggtgacgaac
    10801 acataacggt cgaggaactg ctcgaccggg accatctggt acgaggccgg gtcgccggtg
    10861 ccggcgccgc cgttttggcc ctcgaggtac tgcaccggca tgatcggtcc gctgccgctg
    10921 aagatgaagc tgtccttggt cttgaactcg tggaacttgc ctttattaag agtgatcggg
    10981 aagccgggct gcggcgggat cgtctcgatc gtcgtgttgt cctcgccgga gtacacgcgc
    11041 caccagtagt tctcggtgcc gcgcttgggc gcgtgggcgc cgacgtagac cttgccccaa
    11101 tactcgagcg ggatcgcctg ctcctcgatg tggtcgcaga aggtcacgtt cgcgggcacg
    11161 ttgacgcact gcacggcgcc gaccacccag gccggctcgg acaggctgac gatcgtgccg
    11221 gagaggtcgc tctggcccga gctgatctgc agcaggtcgt agtcgccgac cgtcaccatg
    11281 ccggtctggt tggcggcgac cgccggcacg ccgttgccgg cctgggtcgc caccggcggg
    11341 gtccaggaca ccaccgtgcc gtccttgagg ccgatgacgt tgaagtacgc cgggttgccg
    11401 ccgacgttgg gcgtgtacgc cgggatgatg tagttcttct gctgcgcgtg gtcgggcagc
    11461 agcatcgacg agtcgttgtg cgccatcgcc ttgatcgggc tgtgcaggta cgcgaccagc
    11521 ggcacggtgc tgacgatctt gaaggtcgag tcggggcgca gcacgctgcc gatgcccggc
    11581 tgcggcggat tgagcaggtc gaactgcgcg ctcttgccgg gcgcgacgct gagcgggccc
    11641 gtgacgacct tctcctgccc gccctcgaag aagtacatcg tcacgctcgc ggtcagcgtc
    11701 ttcgagacgt tgccgaccgt caccgcgctc ggctcctcct ggaagttctg catcttgttg
    11761 ccgcgaaaca cgcagccgac gctgctcggg ttgctcttga ccagctcgca cagctccgtg
    11821 caagccccga ggctgcactc ctgcgtcatg ccgcacatga ccggcgggtc gaagccgtcg
    11881 ccctgcgcgt tgcactgctt ggtctggttg gtcccctcgc acacgaccat gttcggctcg
    11941 cacagcttgg cctggcaggc gccgttgaag caggcctggc ccggcggaca gggcaccggc
    12001 gcgaagccct cgcagtcatc ggtgcaggtc agcaggtcgc ccatgtcgca gccgttggtg
    12061 ctgccggggt cgcacacaca gaccttgccg ctcgagctct cggcggcggt cgtcgtcgtg
    12121 tcgccggtgg tcgtctcggt ggtggcggtc gtcgtcgtcg tgccctcgcc ggtcgtcgtc
    12181 gtgccgctga ccgccgtgtc ggtcgcgggg tccgtcgtcg gctcggaggt cgtcgagttg
    12241 gtgccaccca tcgagctctg ggtcgtcatc tcgccggtcg tcggtgtcgt gccctcggtg
    12301 accgtgccgg aggtcgtgac gcccgcggtc gggtcgaccg tcgccgagct cgtgcccgtc
    12361 gtcccggcgg tggtcgtcga cgtgttcccg tcgtcactgc aggccagcgg cgcggcgagg
    12421 aggagcacga gggggaggcg cggagagagt gtcgacatca ggctcagact cccgccagag
    12481 cgcccctcct ggccaccccc gccgagtgcg gatgctctcg gcgcggcttc ggccagcagg
    12541 ccaccacggc ggcgaaatac cggaactccg ggccgcacgc gatgtcggac cgcggtgcgc
    12601 cccggtcagc tgtcctggac cctgctcgtg atggcgtgcg cagctgcgcc ggcgcccacg
    12661 gtcgtgccgg tggccgtgaa ggaggtcgtg ccggtggtca cgccggccgc cgaaccagag
    12721 ccggtcccgg ccgggctcga catcgcggtc gcgtgcgcag acatggaccc ggcggcgccg
    12781 gtgcgcgtgc gcatcgagga cgccgtgctc gagtgccccg agggtcgcct cgagagctgt
    12841 cggggcgaga cgcggttctt cttcgccaac tgcggcgacg agcccgtcac gctgcggggc
    12901 gtcgagctga tcgaccacgg cagccacggc cgcgtgatgt acgacttcga taaccgcgag
    12961 atcgcggcga gcgggatctg gccgtggacg cgcaaggtgt attttgatgg agcttcgcag
    13021 ctcaaggtcg acgtcgtcga cagccacggc gcaccgatca ccgtcgcgga gaagcccgtg
    13081 cgcacctcgc atccgcagcg cgacgcagcc atcgccgcgt gcgtcgcctg caacggcgtg
    13141 tggggcgtgc acgggatgct cccgcgcgag ggttgcaact gccaggcgcg cgacgccttt
    13201 aaaaattgtc acgacggcga cgaatgcgag ggcgcctgtc tgttcgacca atggcagatc
    13261 tcgcagcccg cccagcgcgg cggctgcacg ggtaccggca aacaaactgt ctgcacggcc
    13321 cgcgtgcgcg cgatcggtcg ccccgtcggc cgctgctccg gcttcgtcgg cgtgcgcagc
    13381 tgccataatt tattgcaacg cgggatcgcc tcggagccgg accgggagat accgtggggg
    13441 acgaggcggg tgtgtagcga ttgattcgcg agctgccaat aaggctggac tctcgccgat
    13501 gcccgcgaga tgatgcgtgc cggcttttgc gacgcgtcca tcgggcgcca ctggagatcc
    13561 atgagcggac atgaaacgat cctcggccac ctggcgacgt gggggctctc gcagcgttat
    13621 gaggacacgg ccacggaagg gctcgcgttc ctcctccgca gagatccgac gctgcgcaag
    13681 cagttcgtcg cgctcctcca gaaggcgcag cccggcctcc cggacaatct gcgcttcacc
    13741 acgcaggaga cattcgacgg atgtcgcatc gacatgtgcg gtcgtacgga cagcggcgcg
    13801 ccggtgttca tcgagaacaa gttctgggcg gcgctcaccg ggaaccagcc cgtcgcgtat
    13861 ctcgaacggc tggccagaca gcaggtcccc tcgctgctgc tgttcatcgt gcccgagcga
    13921 cgacgggcct cgctgtggcc cgagctactg tcccgcctga cggcaggcgg ggtcgcgtac
    13981 caggcggctg gcgcacatcg cgtcgagatc gtcgggacgc agcacagcca
//
LOCUS       DASWNJ010000604         2445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_576582, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000604 DASWNJ010000000
VERSION     DASWNJ010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576582"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VGB85_10155"
     CDS             complement(<1..619)
                     /locus_tag="VGB85_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134435.1"
                     /translation="MARSKLDTLIYATILLAPMVTAVGYIASVSGPDDVVVVVEAPTP
                     GPTPVIEPVLEASSPVAEPAPAPVPEASPVPPTIDPEPEVRTFDPVRDPDPLPAVPGA
                     GMLMFEGQVILATNPDTAWSKGRLRAPDLEEGVTATKLADAAKLPAELAPLIEGRVVV
                     YAGDGSACTATTGAMTIYARQDGMLNYDGDGGPLDAAELLRLRKHV"
     gene            complement(689..2290)
                     /locus_tag="VGB85_10160"
     CDS             complement(689..2290)
                     /locus_tag="VGB85_10160"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HEY0134436.1"
                     /translation="MNKIRIVCVVAGIAACGEDGDRDDASASASASITASATVTLTTP
                     GGEPGTTGDASGTDPTGGVVTGGTSMGGSSTVDPEATSATGTTSDVAGCSDASDCPDG
                     QTCQGGECVAAPCTADNVGTGDCVCAPGASEPCYSGPGGTQDVGLCVGGTHVCDVGGT
                     AWGVCEGEVTPANELCGNAIDDNCNGTVDEDVDADGDGFTVCGGDCCDQDGQGCEKGK
                     LVNPGAFEVAGNELDDDCDGMEDNILPDCDGALDVASNDAMDGARAIDICKVSMGAKD
                     WGVVSAKYVQVDGSGPPNVLFDVGHGLVGGFGPNVTPKQGARMLGLSSGAARQPTDPG
                     YQSPGGYGKGFTSAHPQGFPKESPACPGVTTGQPHDSAALELQIRVPTNAYGFAFDFT
                     FYTYEWPSYVCSQFNDFFVAMLDPIPMGQADGNIAFDMMGNPISVNNAFLDVCGCNGG
                     PPCNAGGKQFACPLGTAELTGTGFENHAATSWLTTTAPVVPGSVITIRWGTYDSGDGA
                     LDSTAILDRWRWEIDPNTKVETIPQ"
BASE COUNT          308 a          824 c          930 g          383 t
ORIGIN      
        1 acacgtgctt gcgcaggcgc agcagttcgg ccgcgtcgag cggcccgccg tcgccgtcgt
       61 agttgagcat gccgtcctgg cgcgcataga tcgtcatcgc gccggtggtc gccgtacacg
      121 cgctgccgtc accggcgtac acgacgacgc ggccttcgat cagcggcgcg agctcggcgg
      181 gcagcttcgc ggcgtcggcg agcttggtcg cggtgacgcc ctcctcgagg tcgggggcgc
      241 gcagccggcc cttcgaccac gcggtgtcgg ggttggtcgc gaggatgacc tggccctcga
      301 acatcagcat gcccgcgccg gggaccgccg gcagcgggtc cgggtcgcgc acggggtcga
      361 acgtgcgcac ctccggctcc ggatcgatcg tcggggggac ggggctggcc tcggggacag
      421 gcgcgggcgc gggctcggcg accggtgagc tggcctcgag gacaggttcg atcacggggg
      481 tgggcccggg ggtgggggcc tcgacgacga cgacgacgtc gtccggcccg gacaccgacg
      541 cgatgtagcc gacggcggtc accatcgggg cgaggaggat cgtcgcatag atcagggtgt
      601 cgagcttcga gcgggccatc gcagaaccta cgttcgtcgg ggggagaaga ttcctgcggc
      661 cgggcccgtg cgggaccggc cggcgggctc actgcgggat cgtctcgacc ttggtgttcg
      721 gatcgatctc ccagcgccag cgatcgagga tcgccgtcga gtcgagcgcg ccgtcacccg
      781 agtcgtaggt gccccagcgg atcgtgatca cgctgcccgg gacgaccggc gcggtggtcg
      841 tcagccacga ggtcgcggcg tggttctcga agccggtgcc ggtcagctcc gcggtgccga
      901 gcgggcaggc gaactgcttg ccgccggcgt tgcacggcgg gccgccgttg cagccgcaca
      961 cgtcgaggaa cgcgttgttg acgctgatcg ggttgcccat catgtcgaag gcgatgttgc
     1021 cgtcggcctg gcccatcggg atcgggtcga gcatggcgac gaagaagtcg ttgaactggc
     1081 tgcacacgta gctcggccac tcgtaggtgt agaacgtgaa gtcgaaggcg aagccgtagg
     1141 cgttggtcgg cacgcggatc tgcagctcga gcgcggcgct gtcgtgcggc tggccggtgg
     1201 tgacgcccgg gcaagccggc gactccttcg ggaagccctg cgggtgagcg ctggtgaagc
     1261 ccttgccgta gccgccgggg gactggtagc cggggtcggt cggctggcgc gcggcgccgc
     1321 tcgacaggcc gagcatgcgc gcgccctgct tgggcgtgac gttggggccg aagccgccga
     1381 cgaggccgtg gccgacgtcg aacaggacat tgggcgggcc actgccgtcg acctgcacgt
     1441 acttggccga gacgacgccc cagtccttgg cgcccatcga gaccttgcag atgtcgatcg
     1501 cgcgggcgcc gtccatcgcg tcgttgctgg cgacgtcgag cgcgccgtcg cagtcgggca
     1561 ggatgttgtc ctccatcccg tcgcagtcgt cgtcgagctc gttgccggcg acctcgaagg
     1621 cgccggggtt gacgagcttg cccttctcgc agccctggcc gtcctggtcg cagcagtcgc
     1681 cgccgcacac ggtgaagccg tcgccgtcgg cgtcgacgtc ctcgtcgacg gtgccgttgc
     1741 agttgtcgtc gatcgcgttg ccgcacagct cgttggcggg ggtgacctcg ccctcgcaca
     1801 cgccccacgc ggtgccgccg acgtcgcaga cgtgcgtgcc gccgacgcac aggccgacgt
     1861 cctgcgtgcc gccggggccg ctgtaacacg gctcgcttgc cccgggcgcg cacacgcagt
     1921 cgccggtgcc gacgttgtcg gcggtgcacg gcgccgcgac gcactcgccg ccctggcagg
     1981 tctgtccatc gggacagtcg ctggcgtcgc tgcagccggc gacgtcgctg gtggtgccgg
     2041 tggcggaggt cgcctcgggg tcgacggtgc tgctgccgcc catcgaggtg ccgccggtga
     2101 cgacgccgcc ggtcgggtcg gtgccgctgg cgtcgccggt ggtgccgggc tcgccgccgg
     2161 gcgtcgtcag cgtcacggtg gcgctcgcgg tgatgcttgc gcttgcgctg gcgctcgcgt
     2221 cgtcacgatc gccgtcctca ccacacgcgg cgatacccgc gaccacgcac acgatccgaa
     2281 ttttattcat ggtcaagatc ccccgatctg agcgtagagg cacagcgcgg gccagcgcaa
     2341 cggcgcaggt gtcgcgcgca cagcgtcgtg tgcgcgcttg cgcgcaggct cgaatctagc
     2401 cgggactcta gccggggatc ggcggcatgc acagggcgcc gcctt
//
LOCUS       DASWNJ010000605         2128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_188187, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000605 DASWNJ010000000
VERSION     DASWNJ010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188187"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..1227)
                     /locus_tag="VGB85_10165"
     CDS             complement(190..1227)
                     /locus_tag="VGB85_10165"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY0134437.1"
                     /translation="MQAFTDLRAEIRALGLDQRRATPILRVVLLHLVVFAVSVAAYIA
                     IDALWYRACCLIVMSAAGIGVGTHTHTSAHHATSRRRWLNQTLTYLGYPLFAGFSACW
                     WAHKHHVVHHAAPNVLGVDKDIDLAPYFALTQDEVARSRGLRRLYYRVQWLVFPFALA
                     LNYLNFVAHSWIFVVGRLARGQGRRPALLLDLALMTLHVVVSIGVPAIWLGLSGALWL
                     HLLRYVGLGLGMFLVFAPGHFPAEAVCLGRRAAPGEFAALQISASTNFRAGLVGRLIC
                     GGLEHQIEHHLFPNVSPVHYPRLRALVRQFCARNGLPYHEASWDRAVWRSLAVLRRPK
                     AVADDLGAPAM"
     gene            complement(1246..1971)
                     /locus_tag="VGB85_10170"
     CDS             complement(1246..1971)
                     /locus_tag="VGB85_10170"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY0134438.1"
                     /translation="MSLRAARQHLLGWIFTAVMGTLMMATTLLSLGTLSHPVGRPLLR
                     LWGRGMLAILGVRLVVRGREHLAGRAGRIVPFNHASTLDIFIVQAILPDGGVPVAKRE
                     FIRVPIVGWVVYLLDFILLDRTNRERAAASLRRAADRIGRERLSVIIAPEGTRSRDGS
                     LGPFKLGPMHLAVASHAPLIPMVIAGSARAMPHGRWSSRGGTIVVEFLPPIATDDYTG
                     ENLRERTESLRHTFVAALARLDP"
BASE COUNT          352 a          660 c          864 g          252 t
ORIGIN      
        1 atccgccccg aggtcgcgca gacgctggtg cacatcgacg ccaacctctc gaacatcacg
       61 acgcgcggga ccttgcagag cggcatcgcc tacgcgctcg gccgccgcgg caagaaggag
      121 ctgtagaagc ggcggctgtc cccggggaca gcttcacagg cttcactagc ttcactggct
      181 ttactggctt cacatggcgg gcgcgccgag gtcgtcggcg acggccttgg ggcggcgcag
      241 gacggcgagg gaccgccaca cggcgcggtc ccacgaggcc tcgtggtaag gcaggccgtt
      301 gcgggcgcag aactggcgga cgagcgcgcg cagccgcggg tagtgcaccg gcgagacgtt
      361 ggggaacagg tggtgctcga tctggtgctc gaggccgccg cagatcagcc ggccgacgag
      421 gccggcgcgg aagttggtcg aggccgagat ctggagcgcc gcgaactcgc cgggggcggc
      481 gcgacggccg aggcacacgg cctcggccgg gaagtggccg ggcgcgaaca cgaggaacat
      541 gccgaggccg aggccgacgt agcgcagcag gtgcagccac agcgcgcccg acagcccgag
      601 ccagatcgca ggcacgccga tcgagacgac cacgtgcagg gtcatcagcg cgaggtcgag
      661 gagcagcgcg ggccggcggc cttgacccct ggcgagccgg ccgacgacga agatccagct
      721 gtgcgcgacg aagttgaggt agttgagcgc gagcgcgaac gggaacacca gccactgcac
      781 gcggtagtac agacgcctca ggccgcggct gcgggccacc tcgtcctgcg tgagcgcgaa
      841 gtagggcgcg aggtcgatgt ccttgtcgac gccgagcacg ttgggcgcgg cgtggtggac
      901 gacgtggtgc ttgtgggccc accagcaggc cgagaagccg gcgaacagcg gatagccgag
      961 gtaggtcagg gtctggttga gccagcggcg cctggaggtg gcgtggtgcg cggaggtgtg
     1021 ggtgtgcgtg ccgacgccga tgccggcggc gctcatgacg atcaggcagc aggcgcggta
     1081 ccacagggcg tcgatcgcga tgtacgcggc gacggagacg gcgaacacga cgaggtgcaa
     1141 gagcaccacg cgcaggatcg gcgtggcccg gcgctggtcg aggccgaggg cgcggatctc
     1201 ggcgcggagg tcggtgaagg cttgcatgcg aggatgaggg tgccttcagg ggtcgaggcg
     1261 ggccagcgcg gcgacgaaag tgtgacgaag cgattcggtg cgctcgcgca gattttcgcc
     1321 ggtgtagtcg tcggtggcga tcgggggcag gaattcgacg acgatcgtgc ccccgcgcga
     1381 cgaccagcgc ccgtggggca tcgcgcgggc cgagccggcg atcaccatcg ggatcagcgg
     1441 tgcgtggctg gcgacggcga ggtgcatcgg gccgagcttg aaggggccga ggctgccgtc
     1501 gcgcgagcgg gtgccctcgg gggcgatgat cacggagagg cgctcgcgcc cgatgcggtc
     1561 ggcggcgcgg cgcagcgagg ccgcggcgcg ctcccggttg gtccggtcga gcaggatgaa
     1621 gtcgagcaga tagacgaccc agccgacgat cggcacgcgg atgaattcac gcttggcgac
     1681 cgggacgccg ccgtcgggga ggatcgcctg gacgatgaag atgtcgaggg tcgaggcgtg
     1741 gttgaagggc acgatccggc cggcccgacc cgcgaggtgc tcgcggccgc ggacgaccag
     1801 gcgcacgccg aggatcgcca gcatgccgcg accccacagg cgcagcagcg ggcggccgac
     1861 cgggtgcgag agggtgccga gcgacagcag ggtggtcgcc atcatcaggg tgcccatgac
     1921 ggcggtgaag atccagccga ggaggtgctg gcgagctgct cgaagggaca tgtcctggcg
     1981 gtcactccag cccgggcgcg aacacgcggg cgatcgcggc cgggtcgcgg acggtctgcg
     2041 cggccatcca cgactcggcg tcggcgcgca ggcgggtgcc ctcgtcgcct ccgaccaaca
     2101 ggccgagctg gcgctcggcg acggcggc
//
LOCUS       DASWNJ010000606         1584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_541529, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000606 DASWNJ010000000
VERSION     DASWNJ010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541529"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VGB85_10175"
     CDS             <1..251
                     /locus_tag="VGB85_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134439.1"
                     /translation="TEGSLPAGPSVLLWPGGFGPVAVEKRPDPETQGTFGYESGVSGS
                     DPTCYCDTSPPAPAALLTLTVFTLATLRRPRRAAATRA"
     gene            complement(255..1385)
                     /locus_tag="VGB85_10180"
     CDS             complement(255..1385)
                     /locus_tag="VGB85_10180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0134440.1"
                     /translation="MSHVQAPGPRRTLLAAALFAACTAPAAEVPPPERAPSATIEEGG
                     NIRLDARARAFVVVEAIGNTPTDVTIRAPARVAFRDTAISRVGSPIPARVMKIHVQVG
                     DFVRVGDKLATLASPDASSYHAELAHSRLELTAAKDTLERQTQMVAKGVGREYERVMA
                     EMRVRDVEERVRAAKRHVSLLGKSSGGTVIVNAEIEGTVLARHASVGAQVDPSGEPLF
                     EIGNPSDLWVVADVFQDDLPLVRKGSEVKIELASESDTVPGMVSSVGVLLDTAVRRAP
                     VYIELRDKTTEDLKAGMFALALIRANDIRGITVPVGAVLIKDNNKSIVYVETGEGSFA
                     RRDVNVGHNFEDRVEIVSGLQAGERVAVQGALLIDGAANQLL"
BASE COUNT          209 a          580 c          555 g          240 t
ORIGIN      
        1 agaccgaggg ctctctgccg gccggcccgt ccgtgctgct gtggcccggc ggcttcggac
       61 cggtcgccgt cgagaagcgc cccgacccgg agacgcaggg caccttcggc tacgagagcg
      121 gcgtctcggg ctccgacccg acctgctact gtgacaccag cccgccagca cccgccgcgc
      181 tgctcaccct gacggtgttc acgctggcca ccctccggcg cccgcggcga gccgcggcga
      241 cccgcgcgta ggcctcacag cagctggttg gcggcgccgt cgatcagcag ggcgccctgg
      301 accgcgacgc gctcgccggc ttgcagcccg gacacgatct cgacgcggtc ctcgaagttg
      361 tggccgacgt tgacgtcgcg gcgggcgaag ctgccctcgc cggtctcgac gtagacgatc
      421 gacttgttgt tgtccttgat cagcacggcg ccgaccggca ccgtgatccc gcggatgtcg
      481 ttggcgcgga tcaacgcgag cgcgaacatc ccggccttga ggtcctcggt cgtcttgtcg
      541 cgcagctcga tgtagaccgg ggcgcggcgc accgcggtgt cgagcagcac gccgaccgac
      601 gacaccatgc cgggcacggt gtcgctctcg gaggcgagct cgatcttcac ctccgaaccc
      661 ttgcggacca gcggcaggtc gtcctggaac acgtcggcga cgacccacag gtcgctcggg
      721 ttcccgatct cgaacagcgg ctcgccgctc gggtcgacct gcgcgccgac gctggcgtgc
      781 cgcgcgagca cggtgccttc gatctccgcg ttgacgatca ccgtgccgcc cgagctcttg
      841 ccgagcaggc tgacgtggcg cttggccgcc cgcacccgct cctcgacgtc gcggacccgc
      901 atctcggcca tcacccgctc gtactcgcgg ccgacgccct tggcgaccat ctgcgtctgg
      961 cgttcgagcg tgtccttggc ggcggtcagc tcgagccgcg agtgggccag ctcggcgtgg
     1021 tagctcgacg cgtcggggct ggcgagggtc gccagcttgt cgccgacgcg cacgaagtcg
     1081 ccgacctgca cgtggatctt catcacacgc gcggggatcg gcgagccgac ccgcgagatc
     1141 gccgtgtcgc ggaacgcgac gcgcgcgggg gcccggatcg tcacgtccgt cggcgtgttg
     1201 ccgatcgcct cgaccaccac gaaggcccgc gcacgcgcgt cgaggcggat gttgccgccc
     1261 tcctcgatcg tcgcgctcgg cgcacgctcc ggcggcggca cctcggccgc cggcgcggtg
     1321 caggcggcga acaacgccgc agcgagcagc gtccgtcgcg gaccgggtgc ttgtacgtgg
     1381 gacatggacg gagccttccg gtgcatcagt ggtgggcccc tggtcggggg ctgaagtcga
     1441 gggcgtcgtc ctcgacctgc ttgaggttct tggccgccag caggcggtag agcatgggta
     1501 cgaccagcag ggcgaccagc agggtggtcg ccatgccgca cacgatcacc accgcgaacg
     1561 gcttctgcgt ctcggagccc atcg
//
LOCUS       DASWNJ010000607         1986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_610171, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000607 DASWNJ010000000
VERSION     DASWNJ010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610171"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VGB85_10185"
     CDS             complement(<1..564)
                     /locus_tag="VGB85_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134441.1"
                     /translation="MNSGTLRFLMLLTLAAPACGGDDETSDTSSGSSTSGGGDTTDSS
                     GTTEPTGGTTTDVVTSDTSDTSDTADTTTGTTGSTTGSTDPTGDSTTDDTTDTTDTTT
                     GAIADCGFDPTVAFTRDALVFQLESEDGATCVWLERRDDSDPDVIYKAIPYTLLEWKS
                     GHAGVLTHVTDPAALTWESTHHNWTDVA"
     gene            complement(761..1105)
                     /locus_tag="VGB85_10190"
     CDS             complement(761..1105)
                     /locus_tag="VGB85_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134442.1"
                     /translation="MTTRCLSLMTMIGLLMTGCANKSAGDSQGDAVPPAAGEQPASEQ
                     PAGSTDAERPPLTTAECEAKSGRVVGDIGDGAIHRPDYTCEGGQKPLGTIKPGEGEPV
                     AIEGSVCCPNPA"
     gene            1213..>1986
                     /locus_tag="VGB85_10195"
     CDS             1213..>1986
                     /locus_tag="VGB85_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134443.1"
                     /translation="MVARFSRSQYSPALFDVAQDIVGSLPIKLVERWLHSEQSHEDAC
                     RLLAPMRVVGFNVVSDSAGLTRLTLQRGLLEILALIDQPKEIIHDIGTAIGGEAIGIW
                     AADNTQMFYPQAIAATTLLSALLAVQDAVAQRCQIRIGIAAHLGEYYRLSGGLYGAES
                     DAIEAIAEHDTVGGEIVITQAIVARLPAGHGFTLAPREEQRAPLGPTWRVLDGPRLAA
                     PARARGHYPIPYSAEFHADLLAFERHIEDAGFAQAMTAKY"
BASE COUNT          272 a          700 c          691 g          323 t
ORIGIN      
        1 cgcgacgtcg gtccagttgt ggtgcgtgct ctcccacgtc agcgccgccg ggtcggtgac
       61 gtgcgtgagg acgccggcgt ggccggactt ccactcgagc agggtgtacg ggatcgcctt
      121 gtagatgacg tccggatcgg agtcgtcgcg gcgctcgagc cacacgcagg tcgccccgtc
      181 ctcgctctcc agctgaaata cgagcgcgtc acgggtaaag gcgacagtcg gatcgaagcc
      241 gcaatccgcg atcgcgccgg tcgtcgtatc agtcgtatca gtcgtatcat cggtggtcga
      301 gtcgccggtc gggtcggtac tgccggtcgt actgccggtc gtgcccgtgg tcgtatccgc
      361 ggtatccgaa gtatcggagg tatccgaggt gaccacgtcc gtggtcgtgc cgccggtggg
      421 ctcggtggtg ccgctgctgt cggtggtatc accaccaccg gacgtcgacg accccgagga
      481 ggtgtcgctg gtttcatcgt cgccgccgca ggcgggcgcg gcgagggtga ggagcatcaa
      541 gaagcgaagc gtgccggaat tcatgggagc ctcattcatg ggtagtgcca gcgcgtcgtc
      601 ctcacgagga cggcgcgcgg tgccccgttt gtagcagccc gccggatcgc ctgccacgcg
      661 cgcacctgcg cgaacgcgtg agctactccc ctgacaccgc cgtcacaccg gacgcccgac
      721 tacatcgggc taccggtcga cgcggggaag gccgccgact tcaggccggg ttcgggcaac
      781 agaccgagcc ttcgatcgcc acgggctcac cctcgccggg cttgatcgtg ccgagcggct
      841 tctgtccgcc ctcgcaggtg tagtcggggc gatggatcgc gccgtcgccg atgtcaccga
      901 ccacgcggcc gctcttggcc tcgcactcgg cggtcgtcag cggcggtcgc tcggcgtcgg
      961 tgctgccggc gggctgctcg ctcgcgggct gctcgcccgc ggccggcggc acggcgtcgc
     1021 cctgggagtc accggcggac ttgttcgcgc accccgtcat cagcaggccg atcatggtca
     1081 tcaggctcag gcatcgagtc gtcatggcca tcgctgtagc ccaggcggcc gcgatcgaca
     1141 aggcccggac actggtccgg gcgccgggct ccagcttgac gggcgcgtgc cttcttgctg
     1201 tcctcccgcg cgatggtcgc gaggttcagc cgcagtcagt attcgcctgc gctcttcgac
     1261 gtcgcccagg acatcgtcgg ctcgctgccg atcaagctcg tcgagcggtg gttgcacagc
     1321 gagcagagcc acgaggacgc gtgtcgcctg ctcgcgccga tgcgggtcgt cggcttcaac
     1381 gtcgtgtcgg actccgcggg cctgacgcgg ctgacgctgc agcgcgggct gctcgagatc
     1441 ctcgccctca tcgaccagcc caaggagatc atccacgaca tcggcaccgc gatcggcggc
     1501 gaggcgatcg ggatctgggc cgccgacaac acgcagatgt tctatccgca ggccatcgcc
     1561 gcgacgaccc tgctgtcggc cctgctcgcg gtccaggacg cggtcgcgca gcgctgccag
     1621 atccggatcg gcatcgcggc ccacctcggc gagtactatc gcctcagcgg cggcctgtac
     1681 ggcgccgagt cggacgcgat cgaggcgatc gccgagcacg acaccgtcgg cggcgagatc
     1741 gtgatcacgc aggcgatcgt cgcgcgcctg ccggccgggc acggcttcac gctcgcgccg
     1801 cgcgaggagc agcgcgcgcc gctcggcccg acctggcgcg tgctcgacgg cccgcgcctg
     1861 gccgcgcctg cgcgcgcgcg tggtcactac ccgatcccct actccgccga gtttcacgcc
     1921 gacctgctgg ccttcgagcg ccacatcgag gacgccgggt tcgcgcaggc gatgaccgcg
     1981 aagtac
//
LOCUS       DASWNJ010000608         1932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_681472, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000608 DASWNJ010000000
VERSION     DASWNJ010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681472"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1711)
                     /locus_tag="VGB85_10200"
     CDS             complement(<1..1711)
                     /locus_tag="VGB85_10200"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY0134444.1"
                     /translation="MTADAPETGRDAPAIDPLHRIGVALTELDPDRLIQRLTDEATAM
                     CRAQFGAFFYNVINQRGECYMLYTLSGVPREAFAGFPMPRATEIFRPTFDGTEVLRLD
                     DVLTDPRFGHNAPYKGMPPGHLPVRSYLAVPVFSRTGEVLGGLFFGHAEPGRFTADDE
                     RMMVAVANQTGLALDNARLYAAVEAREQQYRFLADAMPQIVYTSRPNGQLDYVNARWT
                     LYTGLTLAQTQGDGWDLVIHPEDIGGMQASWRSALASGEIFERLYRIRRITDGAYRWH
                     LGRSVPRRNDDGTIVQWVGTSTDVDDQQRSSDALRFLAAASAELSASLDHRNTLANVA
                     KLAIPQVADFCIIAISGTDGALQRLAAAHVEPARLASLQALGDQDLREPDAPGSACQV
                     MRSGRASLIDEIGEAGAASDDPRGSLLHAVGARSWLCVPVLARGRAIGAITFGAAGSG
                     RRFAAADLRVAEDLAVRVSVAVENARLYHDAQEANRAKDQFLATVSHELRTPLTAILG
                     WTRMLRSPQLDDDRRRRGLETIERSARAQTQLIEDLLDISRIISGKMRLEPRSIDPSD
                     PIEA"
BASE COUNT          248 a          621 c          783 g          280 t
ORIGIN      
        1 cggcctcgat cgggtccgac gggtcgatcg accgcggctc gaggcgcatc ttgccgctga
       61 tgatccgcga gatgtcgagc aggtcctcga tcagctgggt ctgggcgcgg gcgctgcgtt
      121 cgatcgtctc gaggccgcgg cggcggcggt cgtcgtcgag ctgggggctg cgcagcatcc
      181 gggtccagcc gaggatcgcc gtcagcgggg tgcgcagctc gtgcgagacg gtggcgagga
      241 attgatcctt ggcgcggttg gcctcctggg cgtcgtggta gaggcgggcg ttctcgacgg
      301 cgacgctgac gcggacggcg aggtcctcgg cgacgcgcag gtcggcggcg gcgaagcggc
      361 ggcccgagcc ggcggccccg aaggtgatcg cgccgatcgc ccggccgcgc gcgaggaccg
      421 ggacacacag ccacgagcgg gcgccgacgg cgtgcaggag gctgccgcgc gggtcgtcgc
      481 tcgctgcgcc ggcctcgccg atctcgtcga tcaggctggc acggccgctg cgcatcacct
      541 ggcacgcgct gccgggggcg tcgggctcgc gcaggtcctg gtcgccgagc gcctgcagcg
      601 acgcgagcct ggccggctcg acgtgggcgg cggcgagccg ctggagcgcg ccgtcggtgc
      661 cggagatcgc gatgatgcag aagtcggcga cctgcgggat cgccagcttg gcgacgttgg
      721 cgagggtgtt gcggtggtcg agcgaggccg agagctccgc gctggcggcg gcgaggaagc
      781 gcagggcgtc gctgctgcgc tgctggtcgt cgacgtcggt gctggtgccg acccactgca
      841 cgatcgtgcc gtcgtcgttg cgccgcggca ccgagcgtcc gaggtgccag cggtaggcgc
      901 cgtcggtgat gcgacggatc cggtacagcc gctcgaagat ctcgcccgag gcgagcgcgc
      961 tgcgccagct ggcctgcatg ccgccgatgt cctcggggtg gatcacgagg tcccagccgt
     1021 cgccctgggt ctgtgccagc gtcagcccgg tgtagagcgt ccagcgggcg ttgacgtagt
     1081 cgagctgccc gttggggcgc gaggtgtaga cgatctgcgg catcgcgtcg gcgaggaagc
     1141 gatactgctg ctcgcgcgcc tcgacggcgg cgtagaggcg cgcgttgtcg agggcgaggc
     1201 cggtctggtt ggcgacggcg accatcatgc gctcgtcgtc ggcggtgaac ctgcccggct
     1261 cggcgtggcc gaagaacagc ccgccgagca cctcgccggt gcgcgagaac acgggcacgg
     1321 cgaggtagct gcgcaccggg aggtggccgg gcggcatgcc cttgtagggg gcgttgtggc
     1381 cgaagcgcgg gtcggtcagg acgtcgtcga ggcgcaggac ctcggtgccg tcgaaggtcg
     1441 ggcggaagat ctcggtcgcc cgcggcatcg ggaagccggc gaaggcctcg cgtggcacgc
     1501 cggacagcgt gtagagcatg taacactcgc cgcgctggtt gatcacgtta tagaagaagg
     1561 cgccgaactg ggcccggcac atcgcggtcg cctcgtcggt caaccgctgg atcaggcgat
     1621 ccggatcgag ctcggtgagc gcgacgccga tacgatgtaa cgggtcgatc gccggcgcgt
     1681 cgcgtccggt ctcgggggcg tcggcggtca tgggtgcagg ccactaggct gggagcggcc
     1741 gtcgatgccg gcaatcgcga gaagcggctg tcgcatgagg gcgccggacg gcgccacggt
     1801 gaccgaccag cgcacctggc gtcgccgccg tcgctcggtg cgcagtcgac gcaatgccgc
     1861 gcgcggccgg catcgccgcg cttgcgcttt gcagtggact gccggacaac tgggtcccgc
     1921 ggtgctcccg gc
//
LOCUS       DASWNJ010000609         2521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_576718, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000609 DASWNJ010000000
VERSION     DASWNJ010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576718"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VGB85_10205"
     CDS             complement(<1..248)
                     /locus_tag="VGB85_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotA"
                     /protein_id="HEY0134445.1"
                     /translation="MEFSTLLANTGGTDFSLAGMWAQMGIIAKFVMVLLLLMFLLSLA
                     LMIERSRAYARAKRQSISYIMLLRNFLQERKLQEAVTAA"
     gene            complement(499..1299)
                     /locus_tag="VGB85_10210"
     CDS             complement(499..1299)
                     /locus_tag="VGB85_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134446.1"
                     /translation="MFDHYLHYQKPDPRRQKRIAIATIISGTMTFAGILFVWAANKMN
                     IVKVDAPTVSFIMVQMSLEEAPPPPPPPPPPAAAEEVEPEKEDIPEEDVPEEEIVQPK
                     DTPDKVPDAKKASGGAKGPAIPGGVPGGVPGGVPGGVLGGVLGGQLGGQLGGVATKRE
                     PTQAAAPKPFSVVVGNAIYKPDPDKNALSATKASMFDKRPGTNETSFCVDVDGKTVDV
                     KTKKKFPNDPKVDEICRDTIKKWRFKPFIVGGKPVKTCSVAEFNFKFN"
     gene            complement(1303..2364)
                     /locus_tag="VGB85_10215"
     CDS             complement(1303..2364)
                     /locus_tag="VGB85_10215"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0134447.1"
                     /translation="MRPILAVVAACALTLMSGCTKIAARDLIREGNTLYHDGKYIDAI
                     AAYDKAAQLEPDGVTLFWNRACAAESIVLKMRDPNQVVDRKLYTDMALSDFKTWIDRL
                     ENKTEADESVYLEHRLALLDADERCDDLLSHWLEKHNAEPKEEGWYSVIARQYDKCGR
                     TKDADQWFVKRTVDFPQSVRAYLSLAIRRLEQLYPEPDSGLQYNANYSDEERIALANE
                     VIALLDKATQIDPKFREAYTYRSIAFTQRQLARRYGDDPTQNTAQENLNRILAREDTM
                     AAWRQQKAICDIDQQPECPADKPPEGPCCMLPARPLTAEEEAADVEQKKLIESEIAAM
                     AAGVEDPAKTKGKGKKGGK"
BASE COUNT          360 a          786 c          904 g          471 t
ORIGIN      
        1 gcggccgtga cggcctcctg cagcttgcgc tcctgcagga agttgcgaag cagcatgatg
       61 tacgagatcg actgtcgctt ggcccgcgcg taggcccggg agcgctcgat catgagggcc
      121 aagctgagca ggaacatcag gagcagcagc accatcacga acttggcgat gatgcccatc
      181 tgcgcccaca tgcccgcgag agagaagtcc gtgcccccgg tgttggcgag cagtgtcgaa
      241 aattccatga gctccatgac ttgctgtctt ctttcgggtc gggtcgggtc aggggtcggg
      301 tcgggccgcg gggggccgga tcaggccttg ggaggggatc gggcccacaa tgggccgtga
      361 ggcaggggtc cggcccggag ggccagatca cggcccggag gggccgcgta ggaggaagga
      421 ggtcaggccg ggagggccgg atcaggcccc ggaggggccg tgaaggaggg aggagatcag
      481 ggccggaggc ccgtgaagtc aattgaactt gaagttgaac tcggcgacgg agcaggtctt
      541 gaccggcttg ccaccgacga tgaacggctt gaagcgccac ttcttgatcg tgtcgcggca
      601 gatctcgtcg accttggggt cgttcgggaa cttcttcttg gtcttcacgt cgaccgtctt
      661 gccgtcgacg tcgacgcaga acgaggtctc gttggtgccg ggacgcttgt cgaacatcga
      721 cgccttggtc gccgacagcg cgttcttgtc gggatccggc ttgtagatcg cgttgccgac
      781 gacgaccgag aagggcttgg gcgccgcggc ctgggtcggc tcgcgcttgg tcgccacgcc
      841 gccgagctgg ccgccgagtt ggccaccgag cacgccgccg agcacaccac ccgggacacc
      901 gccgggcacg ccgccgggca caccgccggg gatcgctggg cccttggcgc cgccggacgc
      961 cttcttggcg tcggggacct tgtcgggcgt gtccttgggc tggacgatct cttcctcggg
     1021 gacgtcctcc tccgggatgt cctccttctc gggctcgacc tcctcggcgg ccgcgggcgg
     1081 cggcggaggc ggcggaggcg gtggcgcctc ctcgagcgac atctggacca tgatgaagct
     1141 gaccgtcggt gcatccacct tgacgatgtt catcttgttg gccgcccaca cgaacaggat
     1201 gccggcgaag gtcatcgtgc ccgagatgat cgtggcgatg gcgatgcgct tttgacgccg
     1261 cggatcgggt ttctgataat gcaggtaatg gtcgaacatc gcctacttgc cgcctttctt
     1321 gcccttgccc ttggtcttgg ccggatcctc aacgccggca gccatcgcgg cgatctctga
     1381 ttcgatcagc ttcttctgct cgacatccgc ggcctcctcc tcggcggtca gcgggcgggc
     1441 cggcagcatg cagcacgggc cctcgggcgg cttgtccgct ggacattcgg gctgctggtc
     1501 gatgtcgcag atcgcctttt gctggcgcca ggcggccatc gtgtcctcgc gcgcgaggat
     1561 gcggttgagg ttctcctggg ccgtgttctg ggtcgggtcg tcgccgtagc ggcgcgcgag
     1621 ctggcgctgc gtgaacgcga tcgatcggta ggtgtacgcc tcgcggaact tcgggtcgat
     1681 ctgggtcgcc ttgtcgagca gggcgatgac ctcgttggcg agggcgatgc gctcctcgtc
     1741 gctgtagttc gcgttgtact gcaggccgct gtcgggctcc gggtagagct gctcgaggcg
     1801 acggatcgcc aggctgaggt acgcgcgcac gctctgcggg aagtcgaccg tgcgcttgac
     1861 gaaccactgg tcggcgtcct tggtgcgccc gcacttgtcg tactggcggg cgatgacgct
     1921 gtaccagccc tcttccttgg gctccgcgtt gtgcttctcg agccagtggc tcagcaggtc
     1981 gtcgcagcgc tcgtcggcgt cgagcagcgc gaggcggtgc tcgaggtaga cgctctcgtc
     2041 ggcctcggtc ttgttctcga ggcggtcgat ccaggtcttg aagtcggaca gcgccatgtc
     2101 cgtgtagagc ttgcgatcga cgacctggtt cggatcgcgc atcttgagga cgatgctctc
     2161 ggcggcgcac gctcggttcc agaacagcgt gacgccgtcc ggctcgagct gcgcggcctt
     2221 gtcgtaggcc gcgatggcgt cgatgtattt gccatcgtgg tagagggtgt tgccctcacg
     2281 gatgagatcg cgcgcagcga tcttggtgca gcccgacatc aaggtcaggg cacacgcggc
     2341 gacgacggcg agaatcggac gcatggagag cgccagtgta cagagatagc gggcgagttc
     2401 aagacgcacg tgcgggggcg tcccactccc gcctgcgcct gtgcggcccg cccaccccat
     2461 gtcgtcgggc ccggcccggc ggacccggcg accaccgcga ccaccgcgag ccggcgacgc
     2521 c
//
LOCUS       DASWNJ010000610         1643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_46758, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000610 DASWNJ010000000
VERSION     DASWNJ010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46758"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..948
                     /locus_tag="VGB85_10220"
     CDS             124..948
                     /locus_tag="VGB85_10220"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEY0134448.1"
                     /translation="MADPTHVPTAAVLLIGNELLSGKIRDQNGHNLAKMMRRRGIRLL
                     EVCTVEDELAAIGAALHRLLERSPLLFTSGGVGPTHDDVTMEAIALALGRPLRRDPAM
                     ERILRRHFGEGITPAALKMADLPEGTELTAEHGWPVMRLSLAGGRRIYILPGIPELCR
                     AKIEALEAIPGELPDAGGWALELLHTAIDEADLAEPLNRIVADYKAVEIGSYPRWTPD
                     EHGNLRCVVRLTFEAIGADRHLAREARDALARALGPAAVIPEATSAGPEQVSESGA"
     gene            1059..>1643
                     /locus_tag="VGB85_10225"
     CDS             1059..>1643
                     /locus_tag="VGB85_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134449.1"
                     /translation="MRRNPPALSFTRTLALGLTVAALGVTATACSQEKKDLLALPAAY
                     DPAGAELTFNKKNLEAFNLMSKDARRQHVKQLKETPGSYSGQAIFQSGNGLAPTVEDS
                     QHGAYELFAHVPDPVLYEITIDYQIFTTPELGRPLAPNHPISFRGTLIDLRFEDETKP
                     RKLTMRVKADSVETITDKTVAGAAAPAPAAPAAPA"
BASE COUNT          249 a          656 c          527 g          211 t
ORIGIN      
        1 atgtctcctc ccccgctgtc gcagccgggt tactccgcgc ccgccgcccg gtcaagcgcg
       61 gcccgccgca gatcgctcac aggcggcccg ggtgctgcta ccctggcgcc gaggagctcg
      121 cgcatggccg accccaccca cgtcccgacc gcggcggtgc tgctgatcgg caacgagctc
      181 ctgagcggca agatccgcga ccagaacggc cacaacctgg cgaagatgat gcgccgtcgc
      241 ggcatccgcc tgctcgaggt gtgcaccgtc gaggacgagc tcgcggcgat cggcgccgcg
      301 ctgcaccgcc tgctcgagcg cagcccgctg ctgttcacct ccggcggcgt cggtccgacc
      361 cacgacgacg tcacgatgga ggcgatcgcc ctcgccctcg gccggcccct gcgccgcgat
      421 ccggcgatgg agcggatcct ccgcagacac ttcggcgagg gcatcacgcc cgccgccctc
      481 aagatggccg acctgcccga gggcaccgag ctgaccgccg agcacggatg gccggtgatg
      541 cgcctgtcgc tcgccggcgg gcgccggatc tacatcctcc cggggatccc ggagctgtgc
      601 cgggccaaga tcgaggcgct cgaggcgatc cccggcgagc tgcccgacgc cggcggctgg
      661 gcgctcgagc tgctgcacac cgcgatcgac gaggccgacc tcgccgagcc cctcaatcgc
      721 atcgtcgccg actacaaggc cgtcgagatc gggtcctacc cgcgctggac ccccgatgag
      781 cacggcaact tgcgctgtgt agttcgtctg accttcgagg cgatcggcgc cgaccgacac
      841 ctcgcgcgcg aggcccgtga cgccctcgca cgcgccctcg gacctgccgc cgtcatcccg
      901 gaggcgacgt ccgcgggacc cgagcaggtg tccgagtccg gtgcctgaca gccccggacc
      961 atgcgcccga gcgcgcgctc acgcgatcgc tcgtcccggg gcagcccatg cgcggcccct
     1021 ggtgcacggg cccggccgga cgtgctagct tcccggccat gcgccgcaac ccccctgcgc
     1081 tctcgttcac ccgcacgctg gccctcgggc tgacggtcgc ggccctcggc gtgaccgcca
     1141 cggcgtgtag tcaggagaag aaggatctgc tggcgctgcc cgcggcctac gaccccgccg
     1201 gcgccgagct gaccttcaac aagaagaacc tcgaggcgtt caacctgatg agcaaggacg
     1261 cgcggcgcca gcacgtcaag cagctcaagg agaccccggg gagctacagc ggccaggcga
     1321 tcttccagag cggcaacggc ctcgcgccga cggtcgagga ctcgcagcac ggcgcctacg
     1381 agctgttcgc ccacgtgccc gacccggtcc tctacgagat cacgatcgac tatcagatct
     1441 tcaccacgcc cgagctcggc cgcccgctcg cgcccaatca tccgatcagc ttccgcggca
     1501 ccctgatcga cctgcgcttc gaggacgaga ccaagccccg caagctgacg atgcgcgtca
     1561 aggccgacag cgtcgagacg atcaccgaca agaccgtcgc tggcgcggcc gctcccgctc
     1621 ccgccgctcc cgccgctccc gcc
//
LOCUS       DASWNJ010000611         3070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_11925, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000611 DASWNJ010000000
VERSION     DASWNJ010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11925"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..537
                     /locus_tag="VGB85_10230"
     CDS             127..537
                     /locus_tag="VGB85_10230"
                     /inference="COORDINATES: protein motif:HMM:NF018738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HEY0134450.1"
                     /translation="MEILDKLKSMVGVGAPTITLVPAAPARAGELLRGTIALRGGEYD
                     TPVTDIAIQLDEMRVVYNALARPEQQFWRRVAEVVIVLEGRTLAAGELLELPFELVMP
                     VDVQPSGAAVSYLLIANTEVPGLNPRTELTLEVA"
     gene            534..1007
                     /locus_tag="VGB85_10235"
     CDS             534..1007
                     /locus_tag="VGB85_10235"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY0134451.1"
                     /translation="MTDDQPGRLEQLSDLNRRFEQIVPHNRALGLRVLALDDARVTFL
                     LPYDLRLVGNPETGVLHGGAISAAMDAACGMAVFQALPRPMIIATLDLRIDYHKPATP
                     HRDVTIRCHCYKVTRSVAFVRGVAYHDDEADPIASGTGSFMLGTRARLKAPGAAP"
     gene            1004..1534
                     /locus_tag="VGB85_10240"
     CDS             1004..1534
                     /locus_tag="VGB85_10240"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY0134452.1"
                     /translation="MSEASGPVDMIDLITAARRAGEISQLVEAVPYASFLGFRVIRVD
                     GELLGHLRFAEHLIGNPYLPALHGGVLGALLESTAIFQLMWETEAIVLPKTITLTVDY
                     LRPGRPVDTFARGILTRQGRRVANVRVEAWQEDRSRPIAVASAHFLIDAAGSPEPPDL
                     PEVSDLPGAAEPPADD"
     gene            1642..>3070
                     /locus_tag="VGB85_10245"
     CDS             1642..>3070
                     /locus_tag="VGB85_10245"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0134453.1"
                     /translation="MGSAAPEDFRARARAEADRYGPDPWVFVRELLQNARDAGARSVV
                     FTTEASDGVWRLRCRDDGEGMSYEHARRYLFALYASSKEARRDQVGRFGVGFWSILRF
                     EPRRIVIRSRPRLASGTGPSGQAEDGAWELELTGDLESAVRRSLTMEPGTELLLERDG
                     PDDELGRRVFEAAWQNARFLGTRDRPDEPLRITVDGKPVNARFELPAPSSAFRRGAVR
                     GVVGLGTAARVELFSRGLRVRAAASLADFTAGGGRHTGRSRVQFAELPGRMAPQALLE
                     SDALELLLSRSDARENKALTRLVRLAERELGRLIARQLDAVRPMSWWRRLLFHARMQL
                     RDSLALRTALAAGIGAVVALIVAWSLWGDHAFTRLRTDLAFLSGGTGETVLVGAAGDL
                     SPRYTDLGRLYRGPQVDVLSGSGVPVELTYEPASARHYFAALVLERFDAEGQAAASDP
                     AKFSTYVGVDASDCPAAAGCTEVALV"
BASE COUNT          411 a         1172 c         1062 g          425 t
ORIGIN      
        1 ggtgcagggg cgcatacagc cgggctttta tcaccggaag cgtgggatcg ccaggaagcg
       61 ccgtgatcgg tcggcgatcg gggatcgggt atagtggtcg cggcgtacac gcaaaggggc
      121 acagcgatgg agatcctcga caaactcaag agcatggtcg gcgtcggcgc gcccacgatc
      181 accctggtac ccgctgctcc cgcccgggcc ggcgagctcc tgcgcggcac catcgccctg
      241 cgcggcgggg agtacgacac ccccgtcacc gacatcgcca tccagctcga cgagatgcgc
      301 gtcgtgtaca acgccctcgc gcggcccgag cagcagttct ggcggcgcgt cgccgaggtc
      361 gtcatcgtgc tcgaggggcg cacgctggcg gccggcgagc tgctggagct gcccttcgag
      421 ctggtgatgc cggtcgacgt ccagccgagc ggcgccgcgg tcagctacct gctgatcgcc
      481 aacaccgagg tcccggggct caatccccgg accgagctga cgctcgaggt cgcgtgacgg
      541 acgaccagcc cgggcgtctc gagcagctca gcgacctcaa ccgccgcttc gagcagatcg
      601 tcccgcataa ccgggctctg ggtcttcggg tgctcgcgct ggacgacgcg cgggtgacct
      661 tcctcctgcc ttacgacctc cgcctcgtcg gcaacccgga gaccggcgtg ctgcacggcg
      721 gggcgatcag cgcggcgatg gacgccgcgt gcggcatggc ggtattccag gccctgccca
      781 ggccgatgat catcgccacg ctcgacctgc gcatcgacta tcacaagccg gcgaccccgc
      841 accgggacgt cacgatccgt tgccactgct acaaggtcac gcgcagcgtc gccttcgtgc
      901 gcggcgtcgc ctaccacgac gacgaggccg acccgatcgc cagcggcacc ggctccttca
      961 tgctcggcac gcgcgccagg ctcaaagcgc cgggggccgc gccatgagcg aagcttcggg
     1021 cccggtcgac atgatcgacc tgatcaccgc ggccaggcgc gccggtgaga tctcgcagct
     1081 cgtcgaggcc gtgccctacg ccagcttcct cggcttccgc gtcatccgcg tcgacggcga
     1141 gctgctcggc cacctgcgct tcgcggagca cctgatcggc aacccgtacc tgcccgcgct
     1201 gcacggcggg gtgctcggcg cgctgctcga gtcgacggcg atcttccagc tcatgtggga
     1261 gaccgaggcg atcgtcttac caaaaacgat caccctgacg gtcgactatc tgcgcccggg
     1321 gcggccggtc gacacctttg cccgcgggat cttgacgcgg cagggccggc gggtcgccaa
     1381 cgtccgcgtc gaggcctggc aggaggaccg cagccggccg atcgcggtcg ccagcgcgca
     1441 cttcctgatc gatgctgccg ggtccccgga gcccccggac cttcccgagg tgtcggacct
     1501 gcccggcgcc gccgaaccgc ccgcggacga ctgatccccc acccacggcg cgcatggcga
     1561 cgcggcatcc ggccgtgcgg cgccgtcgcg agccggtaca ctggaaccgc gtgccgcggg
     1621 cttgccagac aggacgaacc ggtgggtagc gccgcgcccg aggacttccg ggcccgggcc
     1681 cgggccgagg ccgaccgcta cgggcccgac ccgtgggtgt tcgtgcgcga gctgctgcag
     1741 aacgcccgcg acgccggggc gcgcagcgtc gtgttcacga ccgaggccag cgacggcgtg
     1801 tggcgcctgc gctgccgcga cgacggcgag ggcatgagct acgagcacgc ccgccgctac
     1861 ctgttcgccc tctacgcctc gagcaaggag gcgcgcagag atcaagtcgg ccgcttcggc
     1921 gtcggcttct ggtcgattct gcgcttcgag ccgcgcagga tcgtcatccg ctcgcggccg
     1981 aggctggcct ccgggacagg tccttcggga caggccgagg acggcgcgtg ggagctcgag
     2041 ctcaccggcg acctcgagtc cgccgtgcgc cgcagcctga cgatggagcc cggcaccgag
     2101 ctgttgctgg aacgcgacgg acccgacgac gagctcggcc gccgcgtatt cgaggccgcc
     2161 tggcaaaacg cccgcttcct cggcacccgc gaccgacccg acgagccgct gcgcatcacc
     2221 gtcgacggca agccggtcaa cgcccgcttc gagctccccg ccccgagctc cgcgtttcgc
     2281 cgcggggccg tgcgcggcgt cgtcggcctc ggcaccgccg cgcgcgtcga gctgttctcg
     2341 cgcgggctcc gggtccgcgc cgccgcctcg ctcgccgact tcaccgccgg cggcgggcgt
     2401 cacaccggtc gcagccgcgt gcagttcgcc gagctgcccg gccgcatggc cccgcaggcg
     2461 ctgctcgaga gcgacgccct cgagctgctg ctgtcgcgct ccgacgcgcg cgagaacaag
     2521 gccctgacgc ggctcgtacg cctcgccgag cgcgagctcg gccgattgat cgcgcggcag
     2581 ctcgacgccg tgcggccgat gtcgtggtgg cgccgcctgc tgttccacgc gcgcatgcag
     2641 ctgcgcgact cgctcgccct acgtactgcg ctcgccgcgg gcatcggggc ggtcgtcgcc
     2701 ttgatcgtcg cgtggagcct gtggggcgac cacgcgttca cccgcctgcg caccgacctc
     2761 gccttcctgt ccggagggac aggcgagacc gtcctcgtcg gcgccgccgg cgacctctcg
     2821 ccgcgctaca ccgacctcgg ccggctctac cgcggcccgc aggtcgacgt gctcagcggc
     2881 agcggcgtgc ccgtcgagct gacctacgag ccggcctccg cgcgccacta cttcgccgcc
     2941 ctcgtgctcg agcgcttcga cgccgagggc caggccgccg ccagcgaccc cgcgaagttc
     3001 tcgacctacg tcggcgtcga cgcgagcgac tgcccggccg ccgccggctg caccgaggtc
     3061 gcgctggtgg
//
LOCUS       DASWNJ010000612         3110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_223427, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000612 DASWNJ010000000
VERSION     DASWNJ010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223427"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1863)
                     /locus_tag="VGB85_10250"
     CDS             complement(70..1863)
                     /locus_tag="VGB85_10250"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEY0134454.1"
                     /translation="MPSPRRLPLQARPWLVGVSMWLALGCGPGGCHAPQQGSLDSREP
                     AATPVDSSAQGSGGSGRDAARTIVAAPPGPTPMLNDEARAARLFAQRVALGERAPLLE
                     DPEGKPFPSLAPLASLDAQVQPAGFGAPVVASSNLDALPGSRGTGLDGAAAAPAVNGN
                     LLGNFILLGGGGAESLQGFHEALRRLKQGADPDGKVRVLVYGASHTAADVYPGYLRAY
                     LQSRFGAGGQGYVPLVRPSKFYRPTALTLESSRGWKVEHAQTREGRDDGYYGLMGASA
                     SSKPKKKKEYTRVIPAITTTAATGEAGAEVQTSYDVYYLRQPGGGRFKLRIDGKDIAT
                     ISTKAKEVAPGYHVITRPGGPHTVEVLPQGDGEVRLFGMVVENDRPGVVVDTLGIGGT
                     RAANQLKWNEHIWSDNLRRRAPDLVVLAYGTNESVDEDQPIETYRKQLREVLARIHRA
                     APSASCMLIGPGDFPFRAADGTFTPRPRDALIVAAQREVAAEAGCAFWDAMQFMGGEL
                     SMVTWAAAQPAMANSDHLHLSRRGYVRMGMALTDALMFDYDAATVAPPVADTGTQVAR
                     SVESAAPAPGPEDSSARTLDDPPAPEPASRP"
     gene            2424..>3110
                     /locus_tag="VGB85_10255"
     CDS             2424..>3110
                     /locus_tag="VGB85_10255"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0134455.1"
                     /translation="MNWLLRALETLGAVAVYLFAVLRSLFTGPRYLSETVRVADVLVR
                     RCLIPVGLAVAPVGAVISLQGVHIFRSFGAEELLSSLLATAVFREYSPALASVMVAAQ
                     AGSSIAARIGTMKVRGQIDALAVMSVDPIRYVVVPGLIACAVVTPLLNVLTTILGLVS
                     GWFFAVVLTGCDHGSFMANLYSQVSLVDLYVGMGKCLVFGTAVGLIAGYLGLQTSGGA
                     AGVGKAANNTV"
BASE COUNT          391 a         1156 c         1076 g          487 t
ORIGIN      
        1 accaacgagg acgacctgat catgcgcctg caggcctgca acaccgccgg gcagtagtcg
       61 cgaccgcgct cacggcctcg acgccggctc gggcgcgggc gggtcgtcga gcgtgcgcgc
      121 cgagctgtcc tccgggccag gcgcgggcgc ggccgactcg accgagcggg cgacctgcgt
      181 cccggtgtcg gcgaccggcg gcgcgacggt ggcggcgtcg tagtcgaaca tcagggcgtc
      241 ggtgagggcc atgcccatgc gcacgtagcc gcggcgcgac aggtgcaggt ggtccgagtt
      301 ggccatcgcc ggctgggccg cggcccacgt gaccatcgac aactctccgc ccatgaattg
      361 catcgcgtcc cagaacgcgc agccggcctc ggccgcgacc tcgcgctggg ccgcgacgat
      421 cagcgcgtcg cgggggcgcg gcgtgaaggt cccgtcggcg gcgcggaacg ggaagtcacc
      481 gggcccgatc aacatgcagc tcgcggacgg cgccgcgcgg tggatcctcg ccagtacttc
      541 gcgcagttgc ttgcggtagg tctcgatcgg ctggtcctcg tcgaccgact cgttggtccc
      601 gtacgccagc acgacgaggt ccggcgcgcg gcggcgcagg ttgtccgacc agatgtgctc
      661 gttccacttg agctggttgg ccgcgcgggt cccaccgatc ccgagcgtgt cgacgacgac
      721 gcccggccgg tcgttctcga cgaccatgcc gaacaggcgg acctcgccgt cgccctgcgg
      781 cagcacctcg accgtgtgcg ggccgccggg gcgggtgatc acgtggtagc ccggcgcgac
      841 ttccttcgcc ttggtactga tggtagcgat gtccttgccg tcgatcctca gcttgaagcg
      901 gccgccgccg ggctggcgca ggtaatagac gtcgtacgag gtctgcacct ccgcgcccgc
      961 ctcgcccgtc gccgcggtcg tcgtgatcgc ggggatcacc cgcgtgtact ccttcttctt
     1021 cttgggcttg ctcgacgcgc tcgcgcccat cagcccgtag tagccgtcgt cgcggccctc
     1081 gcgggtctgc gcgtgctcca ccttccagcc gcgcgagctc tcgagcgtca gcgcggtcgg
     1141 gcggtagaac ttgctcgggc gcaccagcgg cacgtaaccc tgaccacccg cgccgaagcg
     1201 gctctgcaga taggcccgca ggtaacccgg atacacgtcg gccgccgtgt gcgacgcccc
     1261 gtagaccagc acccggacct tgccgtccgg gtcggcgccc tgcttgagcc ggcgcagcgc
     1321 ctcgtgaaaa ccctgcaagc tctcggcccc gccacccccg agcaggatga agttgccgag
     1381 caggttgccg ttgacggccg gggccgcggc cgcgccgtcg aggccggtgc cccgcgagcc
     1441 gggcagcgcg tcgaggttgc tgctggcgac caccggggcc ccgaagccgg ccggctgaac
     1501 ctgcgcgtcg aggctggcca gcggcgccag gctcgggaac ggcttacctt ccgggtcctc
     1561 gagcagcggc gcccgctccc cgagcgcgac ccgctgcgcg aacaacctgg ccgcccgcgc
     1621 ctcgtcgttg agcatcggcg tcggtcccgg cggtgccgcg acgatcgtgc gcgccgcgtc
     1681 cctgcccgac ccgccggagc cctgcgccga cgagtcgacc ggcgtcgcgg ccggctcgcg
     1741 cgaatcgagg ctgccctgct gcggcgcgtg acaacccccc gggccgcagc cgagcgcgag
     1801 ccacatgctg acgccgacca gccacggccg ggcctgaagg gggagtcggc ggggcgaggg
     1861 cattggggaa accttggctg ttccgggcgc ctcaagtcaa attgttgccc ggcgccggca
     1921 aacctgctgt caccgcggtc ctgcgatcgc caccgacgcg agtcccgcct ctggagcctc
     1981 gccgcgtgcg aatgccggtg cacgcccgct gcaccgccgg tcgctcgcca cgccgccgcg
     2041 agcccccggt cgcctcgccc cggacacgtg tcgccttgtg ccagtgatca cctccggcgg
     2101 ccgctcctgc cgatcgcggc aagggcggca gccgcgggca aaaccgccct ccggccgcct
     2161 cggcccctcg cggccgtgcg agcggcgatt cgcggtaaat tcgtaggtgc atcttcgcca
     2221 agctgttaca ttgaccatgt agtgctgcgc gggaacaaag ccaggtccgg cggagttgag
     2281 gcaggccccc tcggggtcgg caattcggcc aggcgcggag ccgaccacgg cgacgcccac
     2341 gccactcgcc acgccactcg gactacaaca tgttctcccc tctgtagcgc gctcgcctgt
     2401 tgcgctgcgg gaggcgagcg cgggtgaact ggctcctccg agcgcttgag accctgggcg
     2461 ctgtcgccgt ttacctgttt gctgtccttc gttcgctgtt caccggtccg cgctacctca
     2521 gcgagaccgt gcgggtcgcg gacgtgctgg tccgtcgctg tcttatcccg gtcggcctcg
     2581 cggtcgcgcc ggtcggcgcc gtgatctcgc tgcagggtgt gcacatcttc cgcagcttcg
     2641 gcgcggagga gctgctgtcc tcgctgctgg cgaccgcggt gttccgcgag tacagcccgg
     2701 cgctcgcctc ggtgatggtc gcggcgcagg ccggctcctc gatcgcggcg aggatcggca
     2761 cgatgaaggt ccgcggccag atcgacgccc tcgcggtgat gtcggtcgac ccgatccgct
     2821 acgtcgtcgt gcctggcctg atcgcctgcg ccgtcgtcac cccgctgctc aatgtactta
     2881 cgacgatcct cggcctcgtc agcgggtggt tcttcgcggt cgtgctgacc ggctgcgacc
     2941 acggctcctt catggccaac ctctatagcc aggtctcgct cgtcgacctc tacgtcggca
     3001 tgggcaagtg cctggtgttc ggcaccgcgg tcggcctgat cgccggctac ctcggcctgc
     3061 agacctccgg cggcgccgcc ggcgtcggca aggccgccaa caacaccgtc
//
LOCUS       DASWNJ010000613         4082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_294648, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000613 DASWNJ010000000
VERSION     DASWNJ010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294648"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VGB85_10260"
     CDS             <1..688
                     /locus_tag="VGB85_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134456.1"
                     /translation="TFVYQPRLREAMQCGRVRLAVGDRSSLLQFVDLPHGFGHAGDLD
                     LALLLATRREDGDARTTQSLLALEVARSRGGRSSALRVIAEVVDAELCARLRRRARLA
                     GDDRVQVYALESLRAAFLFQSVLVPAFNLVYGELMGPWGQSFARKQVTSPGQGTCSFQ
                     QLARRCLADGELLLGVERRRADGEVVVDLAGGDGNGGRIDLATLVAIWVLAADPSEAK
                     RGSCVDGPVA"
     gene            929..1768
                     /locus_tag="VGB85_10265"
     CDS             929..1768
                     /locus_tag="VGB85_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134457.1"
                     /translation="MANIDRFLDAVRKMREVWAKRPEDEDEDEPTRPSPLGAEYQTLT
                     SSLGLPCPPELVLLGDALADGRVFGDRWELNTPEGAVYTRKELQEWAIDSPCLRPRAQ
                     MLPLFGSDGDHLLLDRDGSIWPWSHEIPTDDEGCVARSLDEALGMMLDGPEIEFQRGY
                     RWGFWSVDGIEYKYTVGHSCETDASSKKSRCETGVSIDLAPHVIRPPGDHHLRFGFPE
                     PPPERPRTAPIGLVTTPRGVVDLRRLEVLTALLRIGRARGWTGRAGPLEMGDATPLLD
                     EIP"
     gene            2104..2721
                     /locus_tag="VGB85_10270"
     CDS             2104..2721
                     /locus_tag="VGB85_10270"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEY0134458.1"
                     /translation="MFKVLLIPALLLLACKSDGASSTPPDAATQPETPPADDVTPQTS
                     EDNGDDASKPEESADPPAKPAAAASKDVLATVEAQANGKTFAELIVLTDLAKQLRSMD
                     GAGFTVLVPTDDAFKSLPKGQIEKWKKNPAELERVLKFHIVAGTHDVGKLGNFRTAPT
                     TLGKDVDVKVEESDVRIGGAKLTETDIKASNGYVHIIDKVLVPKK"
     gene            2718..3641
                     /locus_tag="VGB85_10275"
     CDS             2718..3641
                     /locus_tag="VGB85_10275"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0134459.1"
                     /translation="MSEASLLAVIDHLRARLAAGDDEVRWTALDPDRGRGRYAGEQVE
                     VGGQVYVHRPYRVWVDLAERLGMRMLTPRTVGDGLVELRFERLDESARWQARDVEASE
                     RYGRDSGFQRISKLEDPDLVIDFAEALARTRLPEAPRILELGVNRGDMFALIHALEPA
                     MAGRASFVGVDHSASALALARERFPGENFSFVVADVNDLPALGSFDLVIAMGVLQSPG
                     VDDPALLRRLVQVELGWTGALILGVPNCRYLDGERVHGARQKNFRQPELSLVIKDIAY
                     FKRYLQQHRRQVYVTGRDYLLVTAVAEAPSR"
     gene            complement(3599..>4082)
                     /locus_tag="VGB85_10280"
     CDS             complement(3599..>4082)
                     /locus_tag="VGB85_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0134460.1"
                     /translation="GATFRTCVETGADVARLVFEVTEAHAFPDLKLLRRILDRYRTQG
                     ARVALDDMGSGHTSLLYLAALQPDYVKLDRGLLSGLSPDDPRVPLLGAMIRYAHDLGI
                     RVIAEGVESRTELEIVHGLGADFAQGYFLARPAPLPPGLTPDARSYLDGASATAVTSR
                     "
BASE COUNT          631 a         1269 c         1529 g          653 t
ORIGIN      
        1 gacgttcgtg tatcagccgc gtctgcgcga ggcgatgcag tgcgggcgcg tgcggctggc
       61 ggtcggggac cgcagctcgt tgctgcaatt cgtcgacttg ccgcacggct tcgggcacgc
      121 cggcgacctc gacctggcgc tgttgctggc gacgcgccgc gaggatggag atgcgcggac
      181 gacacagtcg ctgctggcgc tcgaggtggc gcgctcgcgc ggcggccggt cgtcggcgct
      241 gcgggtgatc gcggaggtgg tcgacgcgga gctgtgcgcg cggctgcggc gacgcgcgcg
      301 gctggccggg gatgacaggg tgcaggtgta cgcgctcgag agcctgcgcg cggcgttcct
      361 gttccagtcg gtgctggtgc cggcgttcaa tcttgtttac ggcgagctga tgggcccgtg
      421 ggggcagagc ttcgcgcgca agcaggtgac ctcgccgggg cagggcacgt gtagctttca
      481 gcagctggcg cgccgctgcc tggcggacgg cgagctgctg ctcggggtcg aacgccggcg
      541 tgcggacggg gaggtcgtgg tcgacctggc cggcggggat ggcaatggcg ggcgcatcga
      601 tctggcgacg ctcgtggcga tctgggtgct ggcagcggac ccgtcggagg cgaagcgggg
      661 gagctgtgtc gatgggccgg tcgcgtagtg tgcgtggttt ttagaggatc tactcgactg
      721 agggagccag gatgcccgca tcaaagattg ccatcttcgg ccccccgctc gcggggaaac
      781 cactgccttg gcagcctacg cgaattcgcg ggggttgaag gcgtggaccc gctcacttta
      841 ctgctaacag tatggcccgc ccctgcctgg ctggcacatg caactgtcgc tggtctccca
      901 gtcgaggacg tgtgatacga acgtgcacat ggcgaacatc gaccggttcc tcgacgctgt
      961 gcgcaagatg cgcgaggtgt gggcaaagag gccggaggat gaggatgagg atgagcccac
     1021 caggccgagc ccgctcggcg cggaatacca gacgttgacg tcgagccttg gcctgccctg
     1081 tccgcccgag ctcgtcctgc tcggcgatgc gcttgcagac gggcgcgtct tcggcgaccg
     1141 ctgggagctg aacacaccgg aaggcgcagt ctacacgcgc aaagaacttc aagagtgggc
     1201 gatcgactcg ccgtgcctgc gacccagggc ccaaatgctg ccgctgttcg gcagcgatgg
     1261 tgatcatctg ctgctcgatc gcgacggctc catctggcct tggagccacg agatcccaac
     1321 ggacgatgag ggctgcgtgg cgcgctcgct tgacgaggcg ctcggcatga tgctggatgg
     1381 ccccgagatt gaattccagc gtggataccg ctggggcttt tggtcggtag acggtatcga
     1441 atacaagtat accgtcggac acagctgcga gaccgatgcg tcgtcgaaga agagtcgctg
     1501 cgagaccgga gtatctatcg atctcgcgcc gcacgtgatt cggccgcccg gcgatcacca
     1561 cctgaggttc ggttttcccg agccgccgcc cgagcggccg cgcacggcgc ccatcggtct
     1621 cgtcaccaca ccgcgcggtg tcgtcgatct tcggcgattg gaggtgctga ccgctctcct
     1681 caggatcggg cgagcccgcg gctggaccgg acgcgccggt ccactcgaga tgggcgatgc
     1741 cacgccgttg ctcgacgaga ttccctgacc gccaagcgcc atcgcgttcg tgggcacgat
     1801 agacggcggc caggtgcccg agctcggcgc ggcccaggtc ggcgcgctgg gccgcttcgg
     1861 tctcccgctg gctccggggg acactacccg gaggggctgt agctccccgt ctgcggcact
     1921 ttccttctcc gggatttctc ccagacgggg gttgaagccg gcggacaggc gagaccggag
     1981 gagacaggaa gaaggcgaag agttacgtag ctttggctgt gttccggaag gaagtcgcgg
     2041 ggttgcacaa ttgccccagc cgtaaacttg ccggtttcgc gcgcgctggc tcatagtatc
     2101 cgcatgttca aggtcctcct gatccccgcc ctgttgctgc tggcttgcaa gtccgatggt
     2161 gcctcgagca cgccgcccga cgcggcgacg cagcccgaga cgccgccggc cgacgatgtc
     2221 acgccgcaga cctccgagga caacggtgat gatgcatcga agccggagga gagcgccgat
     2281 ccgccggcga agccggcggc cgcggcgagc aaggacgtgc tggcgaccgt cgaggcccag
     2341 gcgaacggca agacctttgc cgagctgatc gtgctgaccg acctcgccaa gcagctgcgc
     2401 agcatggacg gcgccggctt cacggtgctg gtgccgaccg acgacgcgtt caagagcttg
     2461 cccaagggcc agatcgagaa gtggaagaag aacccggccg agctcgagcg cgtgctcaag
     2521 ttccacatcg tggccggcac ccatgatgtc ggcaagctcg gcaactttcg caccgccccg
     2581 acgacgctcg gcaaggacgt cgacgtcaag gtcgaggagt cggacgtgcg catcggcggg
     2641 gccaagttga ccgagaccga catcaaggcg agcaacggct acgtgcacat catcgacaag
     2701 gtgctggtgc cgaagaagtg agcgaggcgt cgctgctggc ggtgatcgat cacctgcggg
     2761 ctcgcctcgc ggcgggtgac gacgaggtcc gctggaccgc gctcgacccg gaccgcgggc
     2821 gcggccgcta cgcgggcgag caggtcgagg tcggcgggca ggtgtacgtg caccggccgt
     2881 atcgcgtgtg ggtcgacctc gcggagcggc tcggcatgcg catgctgacg ccgcggacgg
     2941 tcggcgacgg gctggtcgag ctgcgcttcg agcggctcga cgagtcggcg cgctggcagg
     3001 cgagggacgt cgaggcgagc gagcgctacg ggcgtgacag cggctttcag cggatctcga
     3061 agctcgagga cccggacctc gtgatcgact tcgcggaggc gctggcgcgc acgcggctgc
     3121 cggaggcgcc gcggatcctc gagctcgggg tcaaccgcgg cgacatgttc gcgctgatcc
     3181 acgcgctgga gccggcgatg gcggggcggg cgagcttcgt cggcgtcgac cacagcgcct
     3241 cggcgctggc attggcgcgt gagcgctttc ccggggagaa cttctcgttc gtcgtcgcgg
     3301 acgtcaacga cctgccggcg ctcggcagct tcgacctcgt gatcgcgatg ggcgtgctgc
     3361 agagccccgg cgtcgacgat ccggcgctgc tgcggcggct cgtgcaggtc gagctcggct
     3421 ggactggagc gctgatcctc ggggtgccga actgtcgcta tctcgacggc gagcgggtcc
     3481 atggcgcgcg acagaagaac tttcgccagc ccgagctttc actggtgatc aaggacatcg
     3541 cgtacttcaa gcgctacctg cagcagcatc gccgacaggt gtacgtcacc ggtcgtgatt
     3601 atctactggt gaccgcggtc gccgaggcgc cgtcgaggta gctgcgagcg tcgggggtca
     3661 ggccgggggg cagcggggcg gggcgggcca ggaagtagcc ctgggcgaag tcggcgccga
     3721 ggccgtgcac gatctcgagc tcggtgcggg actcgacgcc ctcggcgatc acgcggatcc
     3781 cgaggtcgtg ggcgtagcgg atcatcgcgc cgagcagcgg gacgcgcggg tcgtcgggcg
     3841 agaggccgct cagtaaaccc cggtcgagct tgacgtagtc gggctgcagc gcggcgaggt
     3901 acagcaggct ggtgtggccg ctgcccatgt cgtcgagcgc gacgcgggcg ccctgggtcc
     3961 ggtagcgatc gaggatcctt cgcaggagct tgaggtcggg gaaggcgtgg gcctcggtga
     4021 cctcgaagac caggcgcgcc acgtcggcgc cggtctcgac gcaggtgcga aaggtggcgc
     4081 cg
//
LOCUS       DASWNJ010000614         2499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_223466, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000614 DASWNJ010000000
VERSION     DASWNJ010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223466"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VGB85_10285"
     CDS             complement(<1..526)
                     /locus_tag="VGB85_10285"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY0134461.1"
                     /translation="MQLIAWSIPFFVALMALEAWLARRRGLRLYRLPVAVSDLSCGIT
                     SQLFNLATFGASLAIYAWVYQARVFEMDARSAWVWLFAMLAVDLLYYFWHRASHEVNI
                     LWAAHIVHHHSEDYNLAVALRQAFLTGFTSLPFFLPLALLGVPPLVYAAAKALNTLYQ
                     FWIHTELVGRLGPLE"
     gene            1068..1925
                     /locus_tag="VGB85_10290"
     CDS             1068..1925
                     /locus_tag="VGB85_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134462.1"
                     /translation="MRGLMMMICAQALISACGGDEGGGTKTSIDPSDSSGDPTQATSA
                     GSSDTETGGDPTGGVDAHRACDLYLDCLAVVTPTELPNAQQGFGHDGTCWQGDEKSAQ
                     QCLAACQTGLETLHGAHPDEPACSTCQAPSDCPEGTTCRDGECRALPGQGCGNGVIDP
                     DEVCDGNEGCDADCLGPAECSPWTLAGCDAEEVCLAIPAAGDVSCEPSDAGLPTLGES
                     CDSEAYELCAEGLICVPGFFGNCLSSGCCVAFCNFDGDKVCPGDLYCKYTDAGGVGTP
                     GLGYLGYCV"
     gene            complement(1979..>2499)
                     /locus_tag="VGB85_10295"
     CDS             complement(1979..>2499)
                     /locus_tag="VGB85_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY0134463.1"
                     /translation="DRPLEDLQKGWAAAQQRYGFLDGDNACALGASYGGYMVNWIASQ
                     WPDAFKCLVNHDGVFDNRMMYYATEELWFPEWEHKGPYWEQKARHELHNPVNHVDQWK
                     LPMLVIHGGLDYRIPETQGLATFTALQRRGVPSQLLFFPDENHWVLKPANSLKWHETV
                     EGWLDTWLKSGA"
BASE COUNT          409 a          815 c          880 g          395 t
ORIGIN      
        1 gctcgagcgg accgaggcga ccgaccagct cggtgtggat ccaaaattga tagagggtgt
       61 tgagcgcctt cgcggcggcg tacaccagcg gcggcacgcc gagcagcgcg agcgggagga
      121 agaacggcag cgaggtgaag ccggtcagga aggcctgccg cagcgcgacc gcgaggttat
      181 aatcctcgct gtggtgatgc acgatgtgcg cggcccacag gatgtttact tcgtggctgg
      241 cgcggtgcca aaagtaatac aaaaggtcga ccgcgagcat cgcgaacagc cacacccagg
      301 ccgagcgcgc gtccatctcg aacacccgcg cctgatacac ccacgcgtag atcgccaggc
      361 tcgcgccgaa ggtcgccagg ttgaacagct gcgaggtgat gccgcagctg aggtcgctga
      421 ccgccaccgg caagcggtac agccgcagac cgcgacgacg cgcgagccag gcctcgaggg
      481 ccatcagggc gacgaagaag gggatcgacc aggcgatgag ctgcatgact cccctctcct
      541 gtttgagcgt gcgctggtgt cgtgtggctt gcaagcttcg catgcgcgcg ctcgcggcgc
      601 ttgcggcggc tcggggccgc gccggggccc gcgccgggca ttgggctccg ggcggacgat
      661 gcgggcgatg cgggcgatgc gggcgatgcg ggcgacgcgg gcgacgcggg cgacgctacc
      721 cgggtcgacg gcagcggccg ccgctctggg ccctcacaga cgtccgcgag gcgtgcccct
      781 ccatcgcacg tgctcggagg ccgggcctcg atggcacacc tgatgacggg ccggttggcc
      841 ctccagtcgc acacggcgcc cgcgcccggc ccaaaaagcg gagcgctcat gatgccggcc
      901 ggcgggttgg acgacaatat cgtcgcgtgt attgaacggc cgtcgccgcc ggggtctgga
      961 ccgtgctgca cgtcatcggc ccgatcatcg tgcttcgcca gtgaagacga ttacacatcc
     1021 aatgtgcctg gtggctcttc gctgagattg ctgttagaac ggccaccatg cgcggactca
     1081 tgatgatgat ttgtgcgcag gcgctgatca gcgcctgcgg tggcgacgag ggtggcggca
     1141 ccaagacgag catcgacccg agcgactcga gcggcgatcc gacccaggcg accagcgccg
     1201 ggagctcgga caccgagact ggcggtgacc ctactggcgg cgtcgacgcg caccgtgcct
     1261 gtgacctcta cctcgactgc ctggccgtgg tcacgccgac cgagctcccg aacgcccagc
     1321 agggcttcgg gcatgacggc acctgctggc aaggggacga gaagtcggcg cagcaatgcc
     1381 tcgcggcctg ccaaacgggg ctcgagacct tgcatggcgc ccatccggac gagcctgcgt
     1441 gttcgacctg tcaggcgccc agcgactgtc ccgaagggac tacatgcagg gatggcgagt
     1501 gcagggctct ccccgggcaa gggtgcggta acggagtcat cgaccccgac gaggtctgcg
     1561 acggcaacga gggctgcgac gcagattgcc tcggaccggc cgagtgcagc ccgtggactc
     1621 tggccggttg cgacgccgag gaggtgtgcc tggcgatccc ggccgccggc gacgtgagct
     1681 gcgagcccag cgacgccggc ctcccgacgc tcggcgagtc ctgcgactcg gaggcatacg
     1741 agctgtgcgc cgagggcctg atctgcgtgc ccggcttctt cgggaattgc ctctcgtcgg
     1801 gctgctgtgt cgccttctgc aacttcgatg gcgacaaggt gtgccccggc gacctgtact
     1861 gcaagtatac cgacgccggt ggggtcggca ctccggggct cggctacctc ggatattgcg
     1921 tctaaaccgg cgctgagggc acggaggctt cgcctgccgc gccctcgctg actcgtgctc
     1981 acgcgcccga cttgagccag gtgtcgagcc agccctcgac ggtctcgtgc cacttcaggc
     2041 tgttggccgg cttgagcacc cagtgattct cgtccgggaa gaacaggagc tggctcggca
     2101 cgccgcggcg ctgcagggcc gtgaaggtcg cgaggccctg ggtctccggg atgcgatagt
     2161 cgaggccgcc gtggatgacg agcatcggca gcttccactg atcgacgtga ttgaccgggt
     2221 tgtgcagctc gtgcctggcc ttctgctccc agtacgggcc tttatgctcc cactcgggga
     2281 accatagctc ctcggtggcg tagtacatca tacgattatc gaacacgccg tcgtgattga
     2341 cgaggcactt gaaggcgtcg ggccactggc tggcgatcca gttgaccata tagccgccgt
     2401 aggacgcgcc cagcgcgcag gcgttgtcgc cgtcgaggaa gccgtagcgt tgctgcgctg
     2461 cagcccagcc cttctgcagg tcctccagcg gccggtcgc
//
LOCUS       DASWNJ010000615         2566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_46830, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000615 DASWNJ010000000
VERSION     DASWNJ010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46830"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VGB85_10300"
     CDS             complement(<1..592)
                     /locus_tag="VGB85_10300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0134464.1"
                     /translation="MRPTTVSQDRARPEAEADADELLRLAVAGDPRMGTILFDRCEAQ
                     VNRLVWRVLGADEDHDDLVQQVFVALLAGLRRVRDPAALHGWVAAVTINTVRSEIRRR
                     RVRRLLFGESRAIDLAEAPAASHEERELLRRTYAALERLPADERIAFALRFIDERPLA
                     WIAAACDCSLATTKRRIAAAQARFARIAGRDPVLAER"
     gene            715..2307
                     /locus_tag="VGB85_10305"
     CDS             715..2307
                     /locus_tag="VGB85_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134465.1"
                     /translation="MHLAPPSGRHAALATLLICACPALTLDPIVTDGTTGTPPDPATG
                     QVTDGDGTTTAPAITDTSPSTGTPETATGLMPETTTGLATTATTSEAGETTGTTGTDE
                     TTGTTGPSGCLGDIVGNPACGGVTPYCVDGACVSCTELDCIAAEPDKPICAQDLGTCV
                     QCQQNADCAADPPICNQATGTCVQCTEHEQCPATACNLETGLCFPEGPVLYVDNSPDA
                     PTFCSDLKPDQGLSPEKPLCTLQNALGRVVEGQPTTIKIKTGTKPQNGIAELPIGSYT
                     VAMRHYGNTVPSLIISFADPALTINEGNLVFMHRIAVWNTSPSSDPLIACAGGELGAR
                     LWLDQQSIFGGRTAIRANNCRVHVRRSTITGNTIGGIDLAGPDGDLAKLWVENSYITD
                     NNGTKFAAIRMGDVTSADILFSTIALNKSPLAAIECIGNWSGQLTIRNSAIVNPGPLY
                     GPACDANVTTSFQLPDSPDKAGLMSVFSTFADGVFRAKLDGELKDIAVWHIGDPIIDH
                     DGDPRPTVDSSPDYAGADRPSR"
BASE COUNT          442 a         1002 c          791 g          331 t
ORIGIN      
        1 ggcgttcggc cagcaccgga tccctgccag cgatccgcgc gaaccgggcc tgcgccgccg
       61 cgatccggcg cttggtcgtc gccagcgagc agtcgcaagc cgccgcgatc caggccagcg
      121 gccgctcgtc gatgaagcgc agcgcgaacg cgatccgctc gtcggcgggc aggcgctcga
      181 gcgccgcgta ggtgcgccgc agcagctcac gctcctcgtg agacgcggcc ggggcctcgg
      241 cgaggtcgat cgcccgcgac tccccgaaca gcagccgccg cacccgtcgc cgccggatct
      301 cgctgcgcac cgtgttgatc gtcaccgccg cgacccagcc gtgcagcgcc gccgggtcgc
      361 gcacgcgtcg caggcccgcc agcagcgcga cgaacacctg ctggacgagg tcgtcgtgat
      421 cctcgtccgc gccgagcacc cgccacacca gccggttgac ctgcgcctcg cagcggtcga
      481 aaaggatcgt gcccatcctt ggatccccgg cgaccgccag gcgcagcagt tcgtccgcgt
      541 cggcctccgc ctccgggcgc gcgcgatcct gtgagacggt cgtcggtcgc atccgggaag
      601 aggtcgccgc gggcttcgcc gatggctcaa aaaatttcaa gcggcgcccg gtcgccccga
      661 tagcccgcct cgaaaaaaat tgagccgtcc gcgagccgat cacgactccc cggcatgcac
      721 ctcgctcctc cgtccggccg acatgccgcg ctcgctaccc tcctgatctg cgcctgtccc
      781 gcgctgacgc tcgacccgat cgtcaccgac ggcacgaccg ggacccctcc cgaccctgcc
      841 acgggtcaag tgacagacgg cgacggcacc acgaccgcgc cggcgatcac cgacacctcg
      901 ccgagcaccg gcacaccgga gacggccacc ggcctcatgc ccgagacgac caccggactc
      961 gccacgaccg cgacgaccag cgaggccggc gagaccaccg ggactaccgg gaccgacgag
     1021 accaccggca ccaccggccc ctccggctgc ctcggcgaca tcgtcggcaa cccggcgtgc
     1081 ggaggtgtga ccccctactg tgtcgacggc gcctgcgtca gctgcacgga gctcgattgt
     1141 atcgcggccg agcccgacaa gccgatctgt gcgcaggacc tcggtacctg cgtgcagtgc
     1201 caacaaaacg ccgactgcgc cgccgacccg ccgatatgca accaggccac gggcacctgc
     1261 gtgcagtgca ccgagcacga gcaatgcccc gcgaccgcgt gcaacctcga gactggcttg
     1321 tgcttcccgg agggcccggt cctttacgtg gacaacagcc ccgacgcccc cactttttgc
     1381 agcgacctga agcccgacca gggcctgtca cccgaaaagc cgctgtgcac cttgcaaaat
     1441 gcgctcgggc gagtcgtcga gggccagccg acgaccatca agatcaagac cggcaccaag
     1501 ccacaaaacg gcatcgccga gctcccgatc ggcagctaca ccgtcgcgat gcgccactac
     1561 ggcaacacgg tgccgtcgct gatcatctct ttcgcggatc cggcgctgac gatcaacgag
     1621 gggaacctcg tgttcatgca ccggatcgcc gtctggaata cctcgccgag cagcgacccg
     1681 ctgatcgcct gcgccggcgg cgagttgggt gcccgcctgt ggctcgacca acagagcatt
     1741 ttcggcggcc gcaccgcgat ccgcgccaac aattgccgcg tgcacgtgcg tcgctccacg
     1801 atcacgggga acaccatcgg cggcatcgat ctcgccggcc ccgacggcga cctcgccaag
     1861 ctgtgggtcg agaacagcta catcaccgac aacaacggca cgaagttcgc cgcgatccgc
     1921 atgggcgacg tcaccagcgc cgatatcctg ttctccacga tcgcgctcaa caagagcccg
     1981 ctggcggcga tcgagtgcat cggcaactgg tcgggccagc tgacgatccg caattcggcg
     2041 atcgtgaacc cgggcccgct ttacggcccc gcctgcgacg cgaatgtcac gacgagcttc
     2101 cagctccccg attcccccga caaggccggg ctcatgagcg tgttctcgac cttcgccgac
     2161 ggcgtgttta gggccaagct cgacggcgag ctcaaggaca tcgcggtatg gcacatcggc
     2221 gatcccatca tcgaccacga cggcgatccg cggcccaccg tcgacagctc cccggactac
     2281 gcgggcgccg accggccctc gcgctgagtc acagccacac ctcgatcggc acccacgccg
     2341 cgccgtcgcg ggacaccagc cgccgctcga ggtcgaagac atcgaccccg tccgcgtcgg
     2401 ccgcactcag cgtgcgcccg tgctcgtcga gcgacaggcg ccgccacgca ccgaccgcct
     2461 cgaactggag cgccagcccg agctcgccgt cgcgggtcgg atagaggtcg ggctacaccg
     2521 gcccgcgcgg ggccgcgagg acctgcacga acagggcccc tccacg
//
LOCUS       DASWNJ010000616         2236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_505013, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000616 DASWNJ010000000
VERSION     DASWNJ010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505013"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..575
                     /gene="ybeY"
                     /locus_tag="VGB85_10310"
     CDS             66..575
                     /gene="ybeY"
                     /locus_tag="VGB85_10310"
                     /inference="COORDINATES: protein motif:HMM:NF014216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEY0134466.1"
                     /translation="MIHIELTREDPWLDHDWDALAKRAATAAIERTTHGELLTTTAAV
                     EISVRLTTDNEVRHLNHQYRQKDKPTNVLSFPMVQRDLLDTVSQNSDDGELLLGDIVL
                     AHGVCVAEAAERGISVEDHATHLLVHGTLHLLGYDHLVDDEAEAMEEIERDALAALGI
                     GDPYLIRED"
     gene            585..1490
                     /locus_tag="VGB85_10315"
     CDS             585..1490
                     /locus_tag="VGB85_10315"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY0134467.1"
                     /translation="MSEDRSTAGNGDPHENSIWRGLRGLIFGERVDDTLRDQLEEAID
                     RHEDDPAPDAKGDLSPLERQMVRNLLHFGERDAGDVGVPRADIVAVEEQTSFDHLVRL
                     FAEAGHSRLPVYRGELDTIIGMIHIKDVFAILATGAEHPPTLAGLIREPLYVPMSRGA
                     LDLLADMRASHVHLAVVLDEYSGTEGLVTIEDLIEEIVGEIEDEHDEAPVALLVPLDG
                     GAWDADARVELEDVGETIDPRLAEAERDVDTLGGLTALLAGRVPDRGDCLDHPSGWRI
                     EVTEADDRRVHRLRLHPPVPVPEEA"
     gene            1541..>2236
                     /locus_tag="VGB85_10320"
     CDS             1541..>2236
                     /locus_tag="VGB85_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134468.1"
                     /translation="MRKHVLIVLGIFVLLAAAALYWLSRPDVARTPIAQVMGWRPTIG
                     APRQQTFPTIRIAEPVGWPGGATPVAAAGLQVTPFADKLDHPRWLYRLPNGDILVAES
                     NSPPREARGITGWVAGWLMGKAGAGVPSANRITLLRDTNGDGVADQRSAFLTGLNSPS
                     GMVLVGDRLYVANTDALVRFPYKAGDTRITAGAELVRKLPGGGNHWARNVIASPDGSK
                     LYVAIGSASNIAEN"
BASE COUNT          364 a          783 c          755 g          334 t
ORIGIN      
        1 tgcgccaagg cgaacggcgc gaccttgctc tacaagggcg atgatttcgc caggacggac
       61 ctcgcatgat ccacatcgaa ctcacccgcg aagacccgtg gcttgatcac gattgggacg
      121 ctctcgccaa gcgcgccgcg accgccgcga tcgagcggac gacgcatggc gagctgctga
      181 ccacgaccgc cgcggtcgag atctcggtcc ggctgaccac cgacaacgag gtccgccacc
      241 tcaaccacca gtatcggcag aaggacaagc cgaccaacgt cctgtccttc ccgatggtcc
      301 agcgcgacct gctcgacacg gtcagccaga attcggacga cggcgaattg ctgctcggcg
      361 acatcgtcct cgcgcacggc gtgtgcgtcg ccgaggcggc ggagcgcggg atctcggtgg
      421 aggatcacgc cacacacctg ctggtgcacg gcacgttgca tctgctaggc tacgaccatc
      481 tggtggatga cgaggccgag gcgatggagg agatcgagcg cgatgcgctt gctgcgctcg
      541 ggatcggcga cccttatctg atacgcgagg actgacccaa ctcaatgtcc gaggaccgaa
      601 gtaccgccgg caacggcgac cctcatgaaa actcgatctg gcgcggcctg cgcggcctga
      661 tcttcggcga acgcgtcgac gacactctgc gcgaccagct cgaggaggcg atcgatcgcc
      721 acgaggacga tcccgccccc gatgccaagg gcgacctgtc gccgctcgaa cgccagatgg
      781 tgcgcaacct cctccacttc ggcgagcgcg acgcgggcga tgtcggggtg ccccgcgccg
      841 atatcgtcgc ggtcgaggaa cagaccagct tcgatcatct cgtccggctc ttcgcggagg
      901 cggggcatag ccggctgccg gtctatcgcg gcgagctcga tacgatcatc gggatgatcc
      961 atataaagga cgtgttcgcg atcctcgcga ccggcgcgga acatccgccg acgctcgccg
     1021 gcctgatccg cgaaccgctc tacgtgccga tgtcgcgcgg tgcgctcgac ctgctcgccg
     1081 acatgcgcgc gagccatgtc catctcgcgg tggtgctcga cgaatattcg ggcaccgaag
     1141 ggctggtcac gatcgaggac ctgatcgagg aaatcgtcgg cgagatcgag gacgagcatg
     1201 acgaggcgcc cgtcgcgctg ctcgtgccgc tcgacggcgg cgcctgggac gccgatgcgc
     1261 gcgtcgagtt ggaggatgtg ggcgaaacga tcgacccgcg actcgccgag gccgagcgcg
     1321 atgtcgacac gctgggcggg ctcaccgcgc tgctcgccgg ccgggtaccc gaccgcggcg
     1381 actgcctcga tcacccgagc ggctggcgga tcgaggtgac cgaggccgac gatcgccgcg
     1441 tccaccgcct gcggctgcac ccgcccgtgc cggtgcccga agaggcctga cgccaagcgg
     1501 cggattgctc cggcgcggcc cccgtcctat ctccgccagc atgcgcaaac acgtcctcat
     1561 cgtcctgggt atcttcgtcc tgctggcggc ggcggcgctt tactggctgt cgcggcccga
     1621 tgtcgcgcgg acgccgatcg cgcaggtcat gggctggcgc ccgacgatcg gcgcgccgcg
     1681 gcagcagacc tttccgacga tcaggatcgc cgagccggtc ggctggcccg ggggtgcgac
     1741 gccggttgcg gccgctgggc tgcaggtgac gcccttcgcc gacaagctcg atcacccgcg
     1801 ctggctctat cgcctgccca acggcgacat cctggtggcc gaaagcaatt cgccgccgcg
     1861 cgaggcgcgc gggatcaccg gctgggtcgc cggctggctg atgggcaagg cgggcgcggg
     1921 cgtgccctcc gccaatcgca tcacgctgtt gcgcgacacc aatggcgacg gcgtggccga
     1981 tcagcgctcg gcgttcctga ccgggctcaa ctcgccctcg ggcatggtgc tggtcggcga
     2041 ccggctctac gtcgccaaca ccgacgcgct ggtgcgcttc ccgtacaagg ccggcgacac
     2101 gcggatcacc gccggtgccg agctggtgcg gaagctgccc ggcggcggca atcactgggc
     2161 gcgcaacgtg atcgcctcgc ccgacggctc caagctctac gtcgcgattg gctcggcctc
     2221 caacatcgcc gagaac
//
LOCUS       DASWNJ010000617         1782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_259631, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000617 DASWNJ010000000
VERSION     DASWNJ010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259631"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..1487
                     /locus_tag="VGB85_10325"
     CDS             180..1487
                     /locus_tag="VGB85_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134469.1"
                     /translation="MVSPEEPRVVAAKPVGRWATTWQIVETFGFPRAMAFCAWTLLVV
                     STYGSLAGVIGEGELTLFGLVTLRTVPLLLAVLVALPNVALFIIARMILYRFSRRNFS
                     LVFRGEAGEVGPVPATARSQAEWMWIYERSLAQTRYEAGLLPQVAVFGLLSSATLAFC
                     VLGIHLPASQLFPNIADVPTWHIWVAWTVLATATTSFLMTIGRILVRIAGNDATTRTF
                     ANAARSYGLCIVSGGLLACLTVSSTDASTAQASIAMGIAVGLLGDRAFLAVSNRAAAL
                     LGAEPEVVDRSVDLRVIEGLGADDLQRLQEENIGSLHALAFVPVPRLFFNTSFNLQRI
                     CDWQDQALLLVYVGVSRARALREFFQIRGIIDAQRLASVAETLPPEVWQQLVACMSFP
                     AEAHLRQLFARLLTDARIDELRVYRGAALRLDPISASITRLDG"
     gene            complement(1484..>1782)
                     /locus_tag="VGB85_10330"
     CDS             complement(1484..>1782)
                     /locus_tag="VGB85_10330"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0134470.1"
                     /translation="DTALAELGHGKRRIALTVPHFFLAPALIAASDLVITLPERVARI
                     ITANYPLRIVPLPIKVPTFDISLAWHERVHHDPANAWMRDQIVKSLRPASAQSA"
BASE COUNT          228 a          550 c          703 g          301 t
ORIGIN      
        1 gctcgtgcac gcccctcgtt gcagcgggcc atcgtggggc cgccggtgac gcgcggtaca
       61 ggcggcggcg ggggtttgct gcggccctcg gcgatcgtgg ccaccgcgtg cgacgttgat
      121 cgtcgctcgc gcgctggcgc acattggacc gccatcgcct gacaggcgac actggcatcg
      181 tggtgagccc cgaggagccg agggtcgtgg ccgcaaagcc ggtcggtcgc tgggccacga
      241 cgtggcagat cgtcgagacc ttcgggttcc cgcgggcgat ggcgttttgc gcgtggacgc
      301 tgctggtggt ctcgacctac gggtcgctcg cgggcgtgat cggcgagggc gagctgaccc
      361 tgttcgggct ggtgacgctg cggacggtgc cgctgttgct ggcggtgctc gtcgcgctgc
      421 ccaatgtggc gctctttatc atcgcgcgca tgatcctgta tagattctcg cggcgaaact
      481 tcagcctggt gtttcgcggc gaggcgggtg aagtggggcc ggtgccggcg acggcgcgct
      541 cgcaggccga gtggatgtgg atctacgagc gcagcctggc gcagacgcgc tacgaggcgg
      601 ggctgctgcc gcaggtggcg gtgttcgggc tgctgtcgtc ggcgacgctg gcgttctgcg
      661 tgctcgggat ccacctgccg gcgagccagc tgttccccaa catcgcggac gtgccgacgt
      721 ggcacatctg ggtcgcgtgg acggtcctgg cgaccgcgac gacctcgttc ctgatgacga
      781 tcgggcggat cctcgtgcgg atcgccggaa acgacgcgac gacgcggacc ttcgccaacg
      841 cggcgcgcag ctacgggcta tgcatcgtca gcggcgggct gctcgcgtgc ctgacggtgt
      901 cgagcaccga cgcctcgacg gcgcaggcga gcatcgcgat gggcatcgcg gtcgggctgc
      961 tcggggaccg ggcgttcctg gcggtgtcga accgggcggc ggcgctgctc ggcgcggagc
     1021 cggaggtggt cgaccgcagc gtcgacctgc gcgtgatcga ggggctcggg gcggacgacc
     1081 tgcagcgcct gcaggaggag aacatcggct cgctgcacgc gctggcgttc gtgccggtgc
     1141 cgcggctgtt cttcaacacg tcgttcaatc tccagcggat ctgcgactgg caggatcagg
     1201 cgctgttgct ggtgtacgtc ggcgtgagcc gggcccgggc gctgcgcgag ttctttcaga
     1261 tccgcgggat catcgacgcc cagcgcctgg cgagcgtcgc ggagacgctg ccgccggagg
     1321 tgtggcaaca gctcgtcgcg tgcatgagct tccccgcgga ggcgcacctg cgccagctgt
     1381 tcgcgcggct gttgacggac gcgaggatcg acgagctgcg ggtatatcgc ggcgcggcgc
     1441 tgcggctcga cccgatctcc gcctcgatca cccggctcga cggttaggcc gactgggcgg
     1501 aagccggacg cagggacttg acgatctggt cgcgcatcca cgcgttggcg gggtcatggt
     1561 ggacgcgctc gtgccacgcg aggctgatgt cgaatgtggg cactttgatc gggagcggga
     1621 cgatgcgcag cggatagttg gcggtgatga tgcgggcgac ccgctccggc aaggtgatga
     1681 cgaggtcgct ggcggcgatc agcgccgggg cgaggaagaa gtgggggacc gtcagggcga
     1741 tccgccgctt gccgtggccg agctcggcga gcgcggtgtc ga
//
LOCUS       DASWNJ010000618         5111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_46920, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000618 DASWNJ010000000
VERSION     DASWNJ010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5111
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46920"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VGB85_10335"
     CDS             <1..393
                     /locus_tag="VGB85_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134471.1"
                     /translation="MVCARVDHTWPIDLCVPRCDPLAYDCPDVALGSSIMVCAPAAAG
                     FGCVLRGNLDGQPLGQPCADHRDCIAAAHCAPADTVPGCTGQSCCAAYCDRTAPTCPL
                     EPQSCRPWYAPGTAPAGLDDVGRCAATG"
     gene            468..1544
                     /locus_tag="VGB85_10340"
     CDS             468..1544
                     /locus_tag="VGB85_10340"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0134472.1"
                     /translation="MLDNREPTLELHPEWLRVPLGDGEHADFHHRWLRHNCDCDRHPQ
                     TRERTLCSSELPDDLAPRTATLHGDVLEIVWAPDDHRSAYPRAWLRANAYALGREAPP
                     PPTADLSRIVLLGRGRDLAAQVQESLHRVQLHGAAVVRHDPDDPTPPEDATEPLISAF
                     EARGLRVIGTHFGRIEDLRTDNSTNQNTDQLGYTDAAIGLHTDQPFLDHPPRYQLLQG
                     IRTAEHGGENLLADALAAFRHLEAIDAHAAELLTTVPVRFHRKQQSFEREVIAPMIAH
                     DGDHFQIRCSYFTLAPHVLPFARMLDFYRAHDSFVRLVRDPRHHRRVRLEPGDWLLYD
                     NHRTLHARTRFTGARWVRGIYFDV"
     gene            complement(1529..2992)
                     /locus_tag="VGB85_10345"
     CDS             complement(1529..2992)
                     /locus_tag="VGB85_10345"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY0134473.1"
                     /translation="MNLPETGWSRDQILAELATMRTQDLATEGGAAFAYIYDSGEREA
                     LALAVEAYRMFLSANGLDPTAFPSLLRMENEILGFCAAHLGGGPEAAGNFTSGGTESL
                     ILAVKTARDWARAHRPEVTSPEMIVPVSAHAALHKAAHYLGVTVVPTAIDPETFKADV
                     AAIRAAITDNTILIAASAASYAHGVIDPIAEIGAIAQERGLLFHVDACIGGFLLPYLR
                     RLGEDVPAFDLSVPGVSSISMDLHKYGYAPKGASVVLYRDKALRRHQIYACAGWTGYA
                     VVNATVQSTKSGGPIAAAWTLLHAIGDSGYLELARKSLEATRKIVAGVREIAGLRLLG
                     EPAMSLVAFTAEGAQVFTIADAMKARGWLVQPQLQGLGAPASLHLTISAGHLAVVDRF
                     LADLRAAVAEAREAGEDLGLTMLLQAASGMDFTALDDEGFGQALALAGVQNGRLPDRM
                     AGINALLNALPIAAREAALSAFVNDLYVAHAGAQTSK"
     gene            complement(2989..4320)
                     /locus_tag="VGB85_10350"
     CDS             complement(2989..4320)
                     /locus_tag="VGB85_10350"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0134474.1"
                     /translation="MSQPPLRRALCLAYGAGSIGAGIFSTIPGLLLLFYLTDTLAVPA
                     SIAGLAILIPKIWDIITDPIVGAWSDRTQTRWGRRRPFLLAGALTLPPCFMALFMAPV
                     GDPTQSFWWVLIAFCLAAGAFTLFQVPYATMPTEMTSDYHERTTLVAYRMAFLTVGIL
                     AGGALAPALVAAGGGGTAGYAQMSVVLSGVTLVAMLVAFLGTRSAPQTSAVAHTLGLR
                     EQLRVALGYKEYRTLLFAYAIQCVALGAVLAAVPYFVRYTLGGDAGAISVLFVCLVGP
                     AIVTMPLWTAVSRRYGKRVGYLAATVIFAAMAGSLLLAGPGPLWRVGVQIGLLGVGFA
                     GLQVFPFAMLPELIDADARATGLRREGVFTALWFVGEKAGFALGAWLVSGILAATGFL
                     ERRAGELVVQSDSALLGVHLATALAPAILAALSLPLLLHYTEPDRHETRPP"
     gene            4384..>5111
                     /locus_tag="VGB85_10355"
     CDS             4384..>5111
                     /locus_tag="VGB85_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134475.1"
                     /translation="MQQLNRSIATRCLVMLVFACACLLGADAHAKKKKVDGPPKPNRT
                     EFGVLPAVNYDSDLGFGFGAIASVARFAPGFRPYKWRLELLLQATVKRAPGKGTEFPF
                     HDDYLNMDFPGLLANRLRITARVGFRKFANTGYYGFGNDSRSETPWEDIDPEADLAGY
                     QSARRFHKFDHIYPLLQANARIRLWDRSTPEQRRRIEALVGFNATYNIVRPYDDSKLA
                     QDIATLDDGTKDARTLGNLLRGTDP"
BASE COUNT          817 a         2051 c         1574 g          669 t
ORIGIN      
        1 atggtctgcg cccgcgtcga ccacacgtgg ccgatcgacc tgtgcgtgcc gcgctgtgac
       61 ccgctcgcct acgactgccc cgacgtcgcc ctcggctcct ccatcatggt ctgcgcgccc
      121 gcggccgccg gcttcggctg cgtgctgcgc ggcaacctcg acggccagcc gctcggccaa
      181 ccctgcgccg accaccgcga ctgcatcgcc gccgcccact gcgccccggc cgacaccgtg
      241 cccggctgca ccggccaaag ctgctgcgcc gcctattgcg accgcaccgc cccgacctgc
      301 ccgctcgaac cacagagctg ccggccctgg tacgcccccg gcaccgcccc ggccggcctc
      361 gacgacgtcg gccgctgcgc ggccaccggc tgacggccga cggccgaatt cggccgccgc
      421 ccttcgggct tgacgcggct ttccattgtt ctgataactc gtccactatg ttggataacc
      481 gcgagcccac gctcgagctg cacccggagt ggctgcgcgt gccgctcggc gacggcgagc
      541 acgccgactt ccaccatcgc tggttgcggc acaactgcga ctgcgaccgc cacccgcaga
      601 cgcgcgagcg caccctgtgc tcctcggagc tgcccgacga cctcgccccg cgcaccgcga
      661 cgctccacgg cgacgtcctc gagatcgtgt gggcgcccga cgatcaccgc agcgcgtacc
      721 cgcgggcctg gctgcgcgcc aacgcctacg cgctcggccg cgaggccccg ccgccgccga
      781 ccgccgacct ctcgcgcatc gtcctgctcg gccgcggccg cgacctcgcc gcgcaggtgc
      841 aggagtcgct gcatcgcgtg cagctgcacg gcgccgccgt cgtccgccac gaccccgacg
      901 acccgacccc gcccgaggac gccaccgagc ccttgatcag cgccttcgag gcccgcggcc
      961 tgcgcgtgat cggcacgcac ttcggccgca tcgaggacct gcgcaccgac aacagcacca
     1021 accagaacac cgaccagctc ggctacaccg acgccgcgat cggcctgcac accgatcaac
     1081 cgttcctcga tcacccgccg cgctaccagc tgttacaggg gatccgcacc gccgagcacg
     1141 gcggcgagaa cctgctcgcc gacgccctcg ccgcgtttcg ccacctcgag gcgatcgacg
     1201 cccacgcggc cgagctgctg accaccgtcc cggtccgctt ccatcgcaag cagcagagct
     1261 tcgagcgcga ggtcatcgcg ccgatgatcg cccacgacgg cgaccacttc cagatccgct
     1321 gctcctactt caccctcgcg ccgcacgtcc tcccgttcgc gcgcatgctc gacttctatc
     1381 gcgcccacga cagcttcgtc cgcctggtcc gcgacccccg gcaccaccgc cgcgtccgcc
     1441 tcgagcccgg cgactggcta ctctacgaca accatcgcac cctgcacgcg cgcacccgct
     1501 tcaccggcgc gcgctgggtg cgaggcatct acttcgacgt ctgagccccc gcgtgcgcga
     1561 cgtacagatc gttcacgaag gccgacagcg ccgcctcgcg cgcggcgatc ggcagcgcgt
     1621 tgagcagcgc gttgatcccc gccatgcgat ccggcagccg accgttttgc acgcccgcga
     1681 gcgcgagcgc ctggccgaac ccctcgtcgt cgagcgccgt gaagtccatg cccgaggccg
     1741 cctgcaacag catcgtcagg cccaggtcct cccccgcctc gcgcgcctcg gcgaccgccg
     1801 cccgcaggtc cgcgaggaac cggtcgacga ccgccaggtg ccccgccgag atcgtcaggt
     1861 gcaggctcgc cggggcgccg agcccctgca gctgcggctg caccagccag ccccgcgcct
     1921 tcatcgcgtc ggcgatcgtg aacacctgcg cgccctcggc cgtgaacgcg acgaggctca
     1981 tcgccggctc gccgagcagc cgcagccccg cgatctcccg cacgccggcg acgatctttc
     2041 gcgtcgcctc gaggctcttg cgcgccagct cgaggtaacc gctgtcaccg atcgcgtgca
     2101 gcagcgtcca ggccgccgcg atcggcccgc cggacttcgt gctctgcacc gtcgcgttga
     2161 ccaccgcgta tcccgtccag ccggcgcacg cgtagatctg gtgccggcga agtgccttgt
     2221 cgcggtacag caccaccgac gcccccttgg gcgcgtagcc gtacttgtgc aggtccatcg
     2281 agatcgagct gacccccggc accgacagat cgaaggccgg cacgtcctcg ccgagccgcc
     2341 gcaggtacgg caacagaaac ccgccgatgc acgcgtcgac gtggaacagc aagccacgct
     2401 cctgcgcaat ggcgccgatc tccgcgatgg gatcgatgac cccgtgggcg tagctcgccg
     2461 ccgaggccgc gatcaagatc gtgttgtccg tgatcgccgc gcggatcgcc gcgacatcgg
     2521 ccttgaacgt ctccgggtcg atcgccgtcg gcaccaccgt cacgccgagg tagtgcgccg
     2581 ccttgtgcaa cgccgcgtgc gcgctgaccg gcacgatcat ctccgggctc gtgacctccg
     2641 gcctgtgcgc ccgggcccag tcacgcgccg tcttgaccgc gaggatcagg ctctccgtgc
     2701 cgccgctcgt aaaattgccg gccgcctcgg gcccgccgcc gaggtgcgcc gcgcagaacc
     2761 cgaggatctc gttctccatg cgcagcaggc tcgggaaggc cgtcgggtcg agcccgttcg
     2821 ccgacaggaa catgcgatac gcctcgaccg ccagcgcgag cgcctcgcgc tccccggagt
     2881 cgtaaatgta cgcgaacgcg gccccgccct cggtggcgag gtcctgcgtg cgcatcgtgg
     2941 caagctcggc gaggatctgg tcgcgggacc agccggtctc tggcaagttc atggtggcct
     3001 tgtctcgtgt cgatcgggtt cggtgtaatg caacagcagc ggcaagctca gcgccgccag
     3061 gatcgccggc gccagcgcgg tggcgaggtg caccccgagc agggcgctgt cgctctgtac
     3121 caccaactcg cccgcgcggc gctcgaggaa tcccgtcgcc gcgaggatgc cgctgaccag
     3181 ccaggcgccg agcgcgaagc cggccttctc gccgacgaac cacagcgccg tgaacacgcc
     3241 ctcgcggcgc agcccggtcg cccgcgcgtc ggcgtcgatc agctccggca gcatcgcaaa
     3301 ggggaacacc tgcagccccg cgaagccgac cccgagcagc ccgatctgca cgccgacgcg
     3361 ccacagcggc ccgggaccgg cgagcaacaa actccccgcc atcgcggcga agatcaccgt
     3421 cgccgccaga tagccgacgc gcttgccgta ccgccgcgac accgccgtcc acagcggcat
     3481 cgtcacgatc gccggcccga ccaggcatac aaacaacacc gagatcgcgc ccgcgtcccc
     3541 gccgagggtg tagcgcacga agtacggcac cgccgcgagc accgccccga gcgcgacaca
     3601 ctggatcgcg tacgcgaaca gcagcgtgcg atactccttg tacccgagcg ccactcgcag
     3661 ctgctcgcgc aggccgagcg tgtgcgcgac ggccgaggtc tgcggcgcgc tgcgggtccc
     3721 gaggaacgcg acgagcatcg ccacgagcgt gacgcccgag agcaccacgg acatctgcgc
     3781 gtagccggcc gtgcccccgc caccggcagc gacgagcgcc ggcgcgagcg ccccaccggc
     3841 gaggatcccg accgtcagga acgccatgcg atacgcgacg agcgtcgtgc gctcgtggta
     3901 gtcgctcgtc atctccgtcg gcatcgtcgc gtacggcacc tggaacaggg tgaacgcgcc
     3961 ggccgccagg caaaacgcga tcagcaccca ccaaaagctc tgcgtcgggt caccgaccgg
     4021 ggccatgaac agggccatga agcacggcgg cagggtcagc gccccggcca gcaggaacgg
     4081 cctgcgccgg ccccagcgcg tctgcgtgcg atccgaccag gcgccgacga tcgggtcggt
     4141 gatgatgtcc cagatcttcg ggatcaggat cgccagaccg gcgatcgacg ccggcaccgc
     4201 gagcgtgtcg gtgagataga acagcagcaa cagcccgggg atcgtgctga agatcccggc
     4261 cccgatggag cccgcgccgt atgccagaca cagggcgcga cgaagcggcg gttgcgacac
     4321 ctcgccaccc taccacgacc tccaccgatg tcaccccgat cgtcgccgat gcgaccaccc
     4381 ccgatgcaac aactcaacag atcgatcgca acgcgctgcc tcgtcatgct ggtgttcgcc
     4441 tgcgcgtgcc tgctcggcgc cgacgcgcac gccaaaaaga agaaggtcga cggcccaccg
     4501 aagcccaacc gcaccgagtt cggcgtgctg ccggccgtca actacgacag cgacctcggc
     4561 ttcggcttcg gcgcgatcgc cagcgtcgcc cgcttcgcgc ccggctttcg cccctacaag
     4621 tggcgcctcg aattgctgct ccaggcgacc gtcaagcgcg cccccggcaa gggcaccgag
     4681 ttcccgttcc acgacgatta cttgaatatg gacttcccgg gcctgttggc aaaccgcctg
     4741 cggatcaccg cgcgggtcgg ctttcgcaag ttcgccaaca ccggctacta cggcttcggc
     4801 aacgactcgc ggtccgagac cccgtgggaa gacatcgacc ccgaagcgga cctcgccggc
     4861 tatcaaagcg cccggcgctt ccacaagttc gaccacatct acccgctgct gcaagccaac
     4921 gcccgcatcc gcctgtggga ccgctccacc cccgagcagc gccgccgcat cgaggcgctc
     4981 gtcggcttca acgccaccta caacatcgtc cgcccctacg atgacagcaa gctcgcgcag
     5041 gacatcgcca ccctcgacga cggcaccaag gatgcacgca cgctcggcaa cctgctgcgc
     5101 ggcaccgacc c
//
LOCUS       DASWNJ010000619         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_541830, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000619 DASWNJ010000000
VERSION     DASWNJ010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541830"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1988)
                     /locus_tag="VGB85_10360"
     CDS             complement(<1..1988)
                     /locus_tag="VGB85_10360"
                     /inference="COORDINATES: protein motif:HMM:NF016944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEY0134476.1"
                     /translation="MAFKFDGQKIPFGPEPKPLREIWIYHPDFEGVHLRGGKVARGGI
                     RFSDRPDDFRTEIHGLMATQMVKNVLIVPVGAKGGFVLRQAPTDRNELRAAGDKYYQL
                     FVEALLSVTDNVVEGKLVTPSGILHAEGPDPYLVVAADKGTAHLSDTANSVSMRNKFW
                     LDDAFASGGSNGFDHKETGITARGAWETTKRCFRELGIDPETDVITAVGVGDMSGDVF
                     GNGLLRSRTVKLLAAFNHAHVFVDPDPDPERSYVERERLFKLPRSQWTDYDGSVLSKG
                     GGVFMRNAKSLDLSPEARAMLGVGPEQVVSGEDAIRAILMMKVDLCWMGGIGTYVKSR
                     EETHADVGDKANDAVRINSNQLRCRVMTEGANLAITDRGRVAYARAGGQNYTSFLDNS
                     GGVDTSDHEVNIKILFAPLLASGTTTRERRNELLRAAEEAVCDAVLANNRSQSRMVSF
                     DVRRSRKDVFRYSRTLSYLVTNVPFNPDSFGLPTEDELAIRARKGGGRFKADASTLCA
                     YSKMLAYRTLLEEKPLSEGLVDRMVREYFPAVIVEGAGEAIKSHLLRREIATTMVVNH
                     IVDNAGATFFPETLTSTRRSASDVAEAYMSAAQAGDVETIRRELYACEDKVRQEAVYR
                     AMTLIEGALEEATYDLLDRQVALPFDQKVVERARDLLQ"
BASE COUNT          278 a          671 c          709 g          348 t
ORIGIN      
        1 tgctgcagca ggtcccgggc ccgctcgacg accttctggt cgaagggcag cgcgacctgg
       61 cggtcgagca ggtcgtaggt cgcctcctcg agcgcgccct cgatcaacgt catcgcgcgg
      121 tacacggcct cctggcggac cttgtcctcg catgcgtaca gctcgcggcg gatcgtctcg
      181 acgtcgccgg cctgcgccgc ggacatgtac gcctcggcga catcgctggc gctgcgccgc
      241 gtgctggtca gcgtctccgg gaagaaggtc gcgccggcgt tgtcgacgat gtggttgacg
      301 accatcgtcg tcgcgatctc gcggcgcagc aggtggctct tgatcgcctc acccgcgccc
      361 tcgacgatca ccgccgggaa gtactcgcgg accatgcggt cgacgaggcc ctcggacaat
      421 ggcttttcct cgagcagcgt ccggtacgcg agcatcttcg agtacgcgca cagcgtcgag
      481 gcgtcggcct tgaaccggcc gccgcccttg cgcgcgcgga tcgccagctc gtcctcggtc
      541 ggcaggccga agctgtcggg gttgaacggc acgttggtga ccaggtagct cagcgttcgg
      601 ctgtagcgga acacgtcctt gcggctgcgc cggacgtcga agctgaccat gcggctctgc
      661 gagcggttgt tggcgagcac cgcgtcgcac acggcctcct ccgcggcccg caacagctcg
      721 ttgcggcgct cgcgggtcgt cgtgccgctg gcgagcagcg gggcgaacag gatcttgatg
      781 ttgacctcgt ggtccgaggt gtcgacgccg ccggagttgt cgaggaacga ggtgtagttc
      841 tggccgccgg cccgcgcgta ggcgacgcgg ccgcggtcgg tgatcgccag gttggcgccc
      901 tcggtcatca cgcggcagcg cagctgattg gagttgatcc gcacggcgtc gttggccttg
      961 tcgccgacgt ccgcgtgggt ctcctccctg ctcttgacgt aggtgccgat gccgcccatc
     1021 cagcacaggt cgaccttcat catcaggatc gcgcggatcg catcctcgcc gctgacgacc
     1081 tgctcgggcc cgacgccgag catcgcgcgc gcctcgggcg agaggtcgag cgacttggcg
     1141 ttgcgcatga acacgccgcc gcccttcgac agcacgctgc cgtcgtagtc ggtccactgc
     1201 gagcgcggga gtttaaacag gcgctcgcgc tcgacgtagc tgcgctcggg atccggatcc
     1261 ggatcgacga acacgtgcgc gtggttgaag gcggccagca gcttgacggt gcggctgcgc
     1321 agcagcccgt tgccgaacac gtcgccgctc atgtcgccga cgccgaccgc cgtgatcacg
     1381 tcggtctcgg gatcgatgcc gagctcgcgg aagcagcgct tggtcgtctc ccacgcgccg
     1441 cgggcggtga tgccggtctc cttgtgatcg aagccgttcg agcccccgga cgcgaaggcg
     1501 tcgtcgagcc agaacttgtt gcgcatgctg accgagttgg cggtgtcgct gaggtgcgcg
     1561 gtgcccttgt cggcggcgac gacgaggtac gggtccgggc cctcggcgtg caggatgccg
     1621 ctcggcgtga cgagcttgcc ctcgacgacg ttgtcggtga ccgacagcag cgcctcgacg
     1681 aacagctggt agtacttgtc accggccgcg cgcagctcgt tgcggtcggt cggggcctgg
     1741 cgcagcacga agccgccctt ggcgccgacc ggcacgatca gcacgttctt gaccatctgc
     1801 gtggccatca ggccgtggat ctcggtgcga aagtcgtccg gacgatcgct gaagcggatg
     1861 ccgccacgag cgaccttgcc gccgcgaagg tgcacgccct cgaagtcggg gtgatagatc
     1921 cagatctcgc gcaggggctt gggctcgggg ccgaagggga tcttctggcc gtcgaacttg
     1981 aaggccatcg ccggggcctg gtcgcg
//
LOCUS       DASWNJ010000620         3411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_681704, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000620 DASWNJ010000000
VERSION     DASWNJ010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681704"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1741)
                     /locus_tag="VGB85_10365"
     CDS             complement(<1..1741)
                     /locus_tag="VGB85_10365"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HEY0134477.1"
                     /translation="MARAPSADESPAASSKSASTGRAAAKPKATSAPETKTAAKPAKA
                     AAKSKVSAKKETKAPAAKADSKAETKAATKAPAATKAPATKETKAPAAKKVAKKTAAK
                     KKTAAAKKTAAAKKTSANKEAKDVKEPKEAKASKKMGRTLPSVEAVGEPEDDDVEDGE
                     TPAARGKGRQLVIVESPAKAKTINKYLGPGFVVQASVGHIRDLPTKSAKGDKQPVPGV
                     DLEHGFKPTYEILASKSKTVADLKKAARGASDVWFATDLDREGEAIAWHLAEVLGIKP
                     AQAKRVVFNAITQQAVHKAFANPRPIDVDKVNAQQARRILDRIVGYQASPLLWKKVSR
                     GLSAGRVQSVAVRLIVEREREIAAFVPDERWEVTARLSLDPAAGAALGTAWAQFLARV
                     DEKGKGPTIKLQNGWLADHQALEAELVELGGQKWSLGCSSTDPKDLSAEITRVAAAAG
                     LIDVAVTSRENPEGKGPAKTLRTVTGAVDPKVRYRVRSMETKRTSSRAPGPFITSSLQ
                     IAAANTLGFTTQRTMRTAQSLYEGVTIPGEGSVGLITYMRTDSTNLSQEALDQIRGYV
                     QRTYGASYLPEKPN"
     gene            complement(2098..2838)
                     /locus_tag="VGB85_10370"
     CDS             complement(2098..2838)
                     /locus_tag="VGB85_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134478.1"
                     /translation="MNLVKTALTYVAIFSAGALTSVSCFEAEFGNPSFRCSPARSPDC
                     PDGEVCCSDDPATTQGRLPNYFNGKNDDKYGVPIFSGANNPLSSSGMCVATDGIDSPF
                     TTGCPVPCNPTWDAGKRDAICGVGVMCCQMQELDPIKDCVLDPMTNKWRAVTGKDIGA
                     PPKLTTWGQAHTTNQDPLGEQCTVFATSGGTMPVDKTALADCYAQLSVADQRGFCRLS
                     CPCIEDVCDLKNPGTPMKCGGVAPQTTM"
     gene            3123..>3411
                     /locus_tag="VGB85_10375"
     CDS             3123..>3411
                     /locus_tag="VGB85_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134479.1"
                     /translation="MSISSPSFSPSPSSGPRRVLLPALFVLLAACSGAQQTGHHDAPT
                     PDTPATPEVDKAPPASEPGAPPAPVLPKVSHREHPAGQAWVFEAGAEPRKQE"
BASE COUNT          429 a         1163 c         1235 g          584 t
ORIGIN      
        1 cgttcggctt ctccggcagg tacgaggcgc cgtaggtgcg ttgcacgtag ccgcggatct
       61 ggtcgagcgc ctcctgcgag aggttggtcg agtcggtacg catgtacgtg atgagaccga
      121 ccgagccctc gcccggaatc gtcacgccct cgtacagcga ctgggcggtc cgcatcgtcc
      181 gctgggtggt gaagccgagc gtgttggcgg cggcgatctg cagcgacgag gtgatgaagg
      241 ggcccggcgc gcgcgagctg gtgcgcttgg tctccatgct gcgcacgcgg taccggacct
      301 tggggtcgac ggcgccggtg acggtgcgca gggtcttcgc ggggcccttg ccctccgggt
      361 tctcgcggct ggtgacggcg acgtcgatca ggccggccgc cgcggcgacg cgggtgatct
      421 cggcgctgag gtccttcggg tcggtcgagg agcagccgag cgaccacttt tgcccgccga
      481 gctcgacgag ctcggcctcg agggcctggt ggtcggcgag ccagccgttt tgcagcttga
      541 tcgtcgggcc tttgcccttc tcgtcgacgc gcgccaaaaa ctgcgcccac gcggtcccga
      601 gcgcggcgcc ggccgccggg tcgaggctga ggcgggcggt gacctcccag cgctcgtcgg
      661 gcacgaaggc cgcgatctcg cgctcgcgct cgacgatcag gcgcacggcg accgactgca
      721 cgcggccggc cgacaggccg cgcgagacct tcttccacag cagcggcgac gcctggtagc
      781 cgacgatgcg gtcgaggatg cgccgggcct gctgcgcgtt gaccttgtcg acgtcgatcg
      841 ggcgcgggtt ggcgaacgcc ttgtgcacgg cctgctgggt gatcgcgttg aacacgacgc
      901 gcttggcctg cgcgggcttg atcccgagca cctccgcgag gtgccaggcg atcgcctccc
      961 cctcgcggtc aaggtctgtc gcgaaccaca cgtccgacgc gccgcgtgcg gccttcttga
     1021 ggtcggcgac ggtcttgctc ttgctggcga ggatctcgta ggtcggcttg aagccgtgct
     1081 cgaggtcgac gccggggacc ggctgcttgt cgcccttggc cgacttcgtc ggcaggtcgc
     1141 ggatgtggcc gaccgacgcc tgcacgacga agcccgggcc gaggtactta ttaattgtct
     1201 tggccttcgc ggggctctcg acgatcacca gctggcggcc cttgccacgc gcggcggggg
     1261 tctcgccgtc ctcgacgtcg tcgtcctccg gctcgccgac cgcctcgacc gacgggagcg
     1321 tgcggcccat cttcttcgac gccttggcct ccttgggctc cttgacgtcc ttggcttcct
     1381 tgttggccga cgtcttcttc gcggcggccg tcttcttcgc ggcggccgtc ttcttcttcg
     1441 cggcggtctt cttggcgacc ttcttcgcgg ccggcgcctt ggtttccttg gtagccggcg
     1501 ccttggtggc agccggcgcc ttggtggccg ccttggtctc ggccttgctg tccgccttgg
     1561 ccgcgggggc ctttgtttcc ttcttcgcgc tcaccttcga cttcgcggcg gccttggccg
     1621 gcttcgcggc ggtcttcgtc tccggtgcag acgtcgcctt gggcttggcg gccgcccgtc
     1681 cagtcgatgc cgacttggag gaggcggccg gggattcgtc tgcagagggg gctcgagcca
     1741 tggaggtcac gtgacggaga gggggatacc gaaaggcccg ggtttataaa aggcccgggt
     1801 ttataaaggc ccggggtcgg ggcgcgccag ggtcgggcga ccccgccaca tacaagaaca
     1861 ctcacggcgc ggctttgtgt cggcgaacgc cgacgcccgc gcaccgaggt cggtcgcggg
     1921 cccgtccgac ccgtgcgggt catgaccgat cgaggatgct cgaagaggtt cgggaaggga
     1981 tcgtgaagga ggagggcgac cgcgccgggc ggacagggtg actccgccga gtgcccggcg
     2041 caagtccccc tggggtaacc gcgtccgaac cccatcagga ggccgggcgg gcggcgctca
     2101 catcgtcgtc tggggcgcga cgccgccgca cttcatcggg gtgcccgggt tcttcaggtc
     2161 gcacacgtcc tcgatgcagg ggcagctcag gcggcaaaag ccgcgctggt cggcgacgct
     2221 gagctgcgcg tagcagtccg cgagggcggt cttgtcgacc ggcatcgtgc cgccgctggt
     2281 ggcgaagacg gtgcactgct cgccgagcgg gtcctggttg gtcgtgtggg cctggcccca
     2341 ggtggtcagc ttcggcggcg cgccgatgtc cttgccggtg acggcgcgcc acttgttggt
     2401 catcggatcg aggacgcagt ccttgatcgg gtcgagctcc tgcatctggc agcacatcac
     2461 gccgacgccg cagatcgcgt cgcgcttgcc ggcgtcccac gtcgggttgc acggcacggg
     2521 gcagccggtg gtgaacgggc tgtcgatgcc gtcggtcgcc acgcacatgc ccgacgagct
     2581 gagcgggttg ttcgcgcccg agaagatcgg gacgccgtac ttgtcgtcgt tcttgccgtt
     2641 gaagtagttc gggaggcgac cttgcgtggt ggcagggtca tccgaacagc aaacctcgcc
     2701 gtccggacag tccggcgacc tcgcggggct gcagcggaac gaggggttgc caaactcggc
     2761 ctcgaagcac gagaccgagg tcagggcgcc ggcgctgaag atcgcgacat aggtgagggc
     2821 agtcttgacg aggttcaacg acggtctcct tgcggcgagc gtcaacgtgg catagaccgc
     2881 cgcggcttgt aaagctctgc gctttctccc gcggccggac ccaggatcga ccggaccccg
     2941 ctccacgcgc tcgcgcagcg cagatccacc gccctgtccc cggccgggcg tgcgccagcg
     3001 actgtgccgg ctgggatcgc gcgcgcgccc gcggcgcgcg aacgccaggg aactacgtga
     3061 gccttgcgga cgttctttgc cagcccgacg aaccccgggc taatctcgaa atggcctccc
     3121 cgatgtcgat ctcgtccccc tcgtttagcc cgtcaccctc gtcagggccg cgccgcgtgc
     3181 tgctccccgc cctgttcgtg ctgctcgcgg cgtgctcggg ggcccagcag acggggcacc
     3241 acgacgcgcc gacgcccgac acgccggcga cccccgaggt cgacaaggcc ccgcccgcga
     3301 gcgagccggg cgcgccgccg gccccggtgc tgcccaaggt cagccaccgc gagcaccccg
     3361 ccggccaggc ctgggtgttc gaggccggcg ccgagccgcg caagcaggag a
//
LOCUS       DASWNJ010000621        12701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_325293, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000621 DASWNJ010000000
VERSION     DASWNJ010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 12701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12701
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325293"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..1209
                     /locus_tag="VGB85_10380"
     CDS             181..1209
                     /locus_tag="VGB85_10380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134480.1"
                     /translation="MKRFSAHFLVSALVLTACPDGGKDTETTSDTSSGSTTATTGGTT
                     GATDTSTTDTPTSTTNPMTTETATEPTTDPTVTSTTDPTATTTTTDPTATSTTTTTET
                     TDPGTTTGGAGVCGDGVMDEGEACDDSNLMTEISESTMAPLMYDAAACIDDCSLVLSL
                     CGNGTVDAGEACDDGNQDSYDDCTTSCTINDKNYHAPCKRMCNQNCDTDVSSGTFTGC
                     DNVEVPVGAEGVCYVSTKFQLAPRHFAEGECAATAQTCSGGILCPPNIGDYNMLTECP
                     AGTTLVDRTTMAAGITVKTKVCQKTCETDADCRWNAFDSVWAASGEFRCQVTANSNDV
                     KICADAQN"
     gene            1278..2711
                     /locus_tag="VGB85_10385"
     CDS             1278..2711
                     /locus_tag="VGB85_10385"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY0134481.1"
                     /translation="MATSLIPSTRRLRTLAAYAAMLAVAAGVYLFIRGRGEQLVAPAV
                     DVVREAHVAHSSTLATVLLALAVIIVMARGLGHLFQRWFGQPPVIGEIVAGLVLGPSV
                     LGAISPEAAQMLLPAAAAPFLGILSKVGVVLFMFLVGLELDPKMLRGNGHATLAISHA
                     SIVAPFLLGATLALGLYPLYSNSSVSFTVFSLFLGVSLSVTAFPVLARILTDRRVQST
                     PLGVTALTCAAIDDVSAWSLLALVVSVASAQAAGVAWTVGAVVVYLLVMFVAVRPLLV
                     RFAASEDAKTGPLAQSAMAVVFVALLLSAVATEAAGVHALFGAFLLGTFLPHEGRLAE
                     QVRLRLEDAVVVLFLPAFFAFTGMRTQINLVDGATDWLICGLIILMATLGKFGGSFAA
                     ARLTGLGWRESAALGVLMNTRGLMELIVLNVGLDMGVLSPTLFAMLVIMALVTTFATT
                     PVLNMVMGERGFADAPARAPVDVGHRA"
     gene            2752..3087
                     /locus_tag="VGB85_10390"
     CDS             2752..3087
                     /locus_tag="VGB85_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134482.1"
                     /translation="MDALRVRFFPEALARTPGLDAAALTAAIQAHVRAQHARPEQEPN
                     EGPPEEEAVIRLVTPQPDGSFQARFGYWRNRFPSSRYDKSESAEGEVLLDGDGTVRRA
                     ALGPLRDRF"
     gene            complement(3380..4564)
                     /locus_tag="VGB85_10395"
     CDS             complement(3380..4564)
                     /locus_tag="VGB85_10395"
                     /inference="COORDINATES: protein motif:HMM:NF025059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY0134483.1"
                     /translation="MLREVNLQRLERTISPHLRRGWTRAVFAAPQGDGTTPPPSAPAE
                     QVVPGPGAGVPPTPEQLAALFTAARSAANAGLVKHTYVIDETRRVQIDARHGRPSVRT
                     LDEAAARKVMGGKDRVLRPDASADLLRAIGIMNADGSISATHARKYKQVNHLAELCRP
                     TWERLAAARPRSAETPLRALDLACGNAYLSFVLAEVLRRESVPLRLHGVDVREDVVAR
                     SRERAATLGMDGLSFARASIAEAEASAAGALGGPPDLVLALHACDTATDEALALAIRL
                     GVPAVLCVPCCQHELAAQLEKRDPGTAPVPAMLEHGLLRRAYADLLTDGLRVEILAAC
                     GYEVTVVEFVSSEHTAKNILLRAHRRHPYAPVDRDRWRLAPIAERCAALGVSPDLLRR
                     LG"
     gene            4945..6174
                     /gene="serA"
                     /locus_tag="VGB85_10400"
     CDS             4945..6174
                     /gene="serA"
                     /locus_tag="VGB85_10400"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEY0134484.1"
                     /translation="MSSPNILLLESIHATAHEEFARHGVVVHTATGAMNEKQLVAALQ
                     ALPQDGPVMLGIRSKTQVTPAVLEAVPQLLAIGAFCIGTDQIALADARRRGVAVFNAP
                     FSNTRSVAELVLGEIIMLSRQVFARSAACHRGLWHKSADGSHEVRGKTLGIVGYGHIG
                     SQLSVLAEALGMQVRYYDILSKLPLGNAQPCNSLHELLGIADFVTLHVPDTELTRGMF
                     GAAEIAVMRPGAYLINASRGMVVDLAALRDGLERGRLAGAAIDVFPSEPAKVGDVFDS
                     PLRGLDSVILTPHIGGSTQEAQANIGREVATSLAAYLTSGSTTGCVTLPNVDAAYIRG
                     GARILNIHRNVPGVLSAVNQLLADSGVNITGQSLATIEAIGLLHVDVPLRPDDPKAHQ
                     LGAAIAALPTSLRTRVI"
     gene            6429..7052
                     /locus_tag="VGB85_10405"
     CDS             6429..7052
                     /locus_tag="VGB85_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134485.1"
                     /translation="MALSPTACKRSGDSNNPGGECGGMCGKGTRCDGNMCVVDYSQDI
                     CSQPEEVNETPVVPMKPPITSWGECYQDRSELPKKFKAVDDKSIPQFDPDKPRPVDMN
                     GNSGDEQLNEALLNANMRDIEYAINECLATAACYQGSSLPDGRIDFVFRLLGTGKIDA
                     ITVTAPTGLSVFGIVPCARKAIADHTFPSYDGPPMTVRYNIQIGGGD"
     gene            complement(7066..7245)
                     /locus_tag="VGB85_10410"
     CDS             complement(7066..7245)
                     /locus_tag="VGB85_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134486.1"
                     /translation="MSKTEGSSTLLPEPFEVRKDAVEKAAPAARAAAARISQVYRRHA
                     EAPVEPDPSDETPAR"
     gene            complement(7327..7740)
                     /locus_tag="VGB85_10415"
     CDS             complement(7327..7740)
                     /locus_tag="VGB85_10415"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY0134487.1"
                     /translation="MPRRVKPSSRAHRLLLWCAATDRSFDLAEQQGAAVLTGKCIHCG
                     ARHVIDLDGHPRTRASVEHIIPRHHGGTDDLRNLAVACERCNYAKGTRLDARRRDDPT
                     LQRVIETLQTRRAERMRPPLSGLDLPPLAAPTDDE"
     gene            complement(7724..8638)
                     /locus_tag="VGB85_10420"
     CDS             complement(7724..8638)
                     /locus_tag="VGB85_10420"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY0134488.1"
                     /translation="MPDDPGLDPGLHSGLLEFALAAARAAGDVLLEHFEGELTVSTKS
                     SDIDLVTSADRAAEATLTERITRAHPSHWILAEESGRLTGDADVADPRPPPGVLQWVI
                     DPLDGTTNFTHRFPHFSVSLALVDHEGPLLGVVHDPTREETFYAVRGRGAFLDSARAR
                     ARNGRPQPLRVTPTRALRLALVATGFAYTRATTPTNNLLEFNRVVPQVRCIRRAGSAA
                     LDLAYVAAGRLDAYWEYHLQPWDVAAGALLVQEAGGELTRIDGGRWDLRHGDLVAAGP
                     TLAPVLQAELAGARTTSGTCPQGHAPAR"
     gene            complement(8785..9285)
                     /locus_tag="VGB85_10425"
     CDS             complement(8785..9285)
                     /locus_tag="VGB85_10425"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY0134489.1"
                     /translation="MSGPSPGQRAPEIDAVSSTGQRFVLSQQAGLCTVVYFFPKAFTP
                     GCTAETKLFRDNYVELLLAGANLVGISTDDEKTQCDFATSLKAPFPIVPDADHKICRS
                     YDVLWPVMNVARRVTVIVRPDMIVDAVFHHEVQVTRHRDDVLRHVNNMFKQARGSSDA
                     PLGARG"
     gene            9373..10860
                     /locus_tag="VGB85_10430"
     CDS             9373..10860
                     /locus_tag="VGB85_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0134490.1"
                     /translation="MSALARVTPGSAGAGRPTRAHAVAAAIAVWVVLSLGLPLWQLGQ
                     EALAVAGPAAPTWVFVLAGVRALALALAVAGIAVVCAYPVARAVPSPVLLMLLLVSPL
                     ARALGVLGLGFVPGALAVILAQSSGAIPLAALVLQVRLRGRPVALLEAAADLGAGPWR
                     RFRAIELPLLGPAFALAAGWSALVSIGDVTTLELAGGGKLYTLALLLRDAVLHEANPR
                     RALAIVAVMLAVAVPCALAMARALAGLAGESSGGDRRPGRNLTVLGWCGVALSATPML
                     GLLASVTRGTGEGTGAMMLRGMLARSLVLTLACGAIAASLGFALALARRPSQAGRDPV
                     AALLLLPLALPPVVYGALMLPAGRLLGLGPGPALTLLGLLPMRVALAYAGAVLAVAAV
                     QREVIDGARDLGAGPMARLLRIWWPLLAPAGVALALVGFAHALADGSVPAFTAGPGGS
                     TLAVGMAIVARGGEVDVVPRWALGLSLAPVGAVWLAVWTGARAAR"
     gene            10947..>12701
                     /locus_tag="VGB85_10435"
     CDS             10947..>12701
                     /locus_tag="VGB85_10435"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0134491.1"
                     /translation="MVKELDPLDVTLMGDTEAPATREVTPVSGLYAGRYQIEREVGRG
                     GMGTVYRALDVMVGDVVALKVLDKSVTGASDQMEWFRREVRLARRITHPNVARTHDMG
                     EQGGAHYITMEYVEGTTLQDLLRARDDEGSEGRRRRQALEPARAARIALAVGEGLAAA
                     HAAGVVHRDLKPANVLIDRSGRVVLTDFGIARALSDEGAGARTMGVVGTPLYMAPEQL
                     AGTPVDVRADIYALGLITFEMLTGALPFAGETPLASALARLTQPPPDPRGASPGLPAP
                     LCELVLQCLARTAAERPDSALAVAERLRAWLIAAGEPLVGLPTATMFGAGAMPEAASA
                     ISYSNLTPTQPRAVTTAVPGAYTQAGLTQNGTRGGPGMTGMTGMTGMTQRPQRPLAPV
                     PTAERALAVLPFRYIGPPDQSYLGDALTDELIDVLSRTRGLRVLGSGATAKYRNERDP
                     RAVGKDLGVDAVVDATLQASPKQVRVAVRLVEVATGTQLWSERFESGLEDLFELQDRI
                     SKRIAEALRVELSTAAGSMDAPAEALSLYLRGRRQLLAFHVTGPDGAIELLEAAIAMA
                     PQLDPAIACHAIACLRAWF"
BASE COUNT         1708 a         4133 c         5013 g         1847 t
ORIGIN      
        1 cacgtcgacc atcggggtga cgttgatctc gttgatcggg tcgccctcat ctccgccgac
       61 gctgactgcc atggggccca agctaccggg gcgcggcgcg ggtgtccagt gtaaactgcg
      121 cgactggcgc tgtgatccgc agcacagttt gtacagatgc ggggcgccta aagtaacgcg
      181 atgaagcgct tttctgccca ttttttggtt tcagccctgg tcctgaccgc ctgccccgac
      241 ggcggcaagg acaccgagac cacgagcgac acgagcagcg gctcgaccac cgcgaccacg
      301 ggaggcacca cgggcgcgac ggacacgtcg acgacggaca cgccgacctc gaccaccaac
      361 ccgatgacga cggagacggc gaccgagccg acgacggacc cgacggtgac ctcgacgacg
      421 gacccgacgg cgacgacgac gacgacggac ccgacggcga cctcgacgac gacgacgacc
      481 gagacgacgg acccggggac gacgaccggc ggcgcgggtg tgtgtggtga cggcgtgatg
      541 gacgagggcg aggcctgcga cgacagcaac ctgatgacgg agatctcgga gtcgacgatg
      601 gcgccgctga tgtacgacgc ggccgcgtgc atcgacgatt gcagcctggt gctctcgctg
      661 tgcggcaacg gcacggtcga cgccggcgag gcctgcgacg acggcaacca ggacagctac
      721 gacgactgca cgacctcgtg cacgatcaac gacaagaact accacgcgcc gtgcaagcgc
      781 atgtgcaacc agaactgcga cacggacgtc agctcgggca cgttcaccgg ctgcgacaac
      841 gtcgaggtgc cggtcggggc ggagggcgtg tgctacgtga gcaccaagtt ccagctggcg
      901 ccgcggcact tcgccgaggg tgagtgcgcc gcgacggccc agacctgctc gggcggcatc
      961 ttgtgcccgc cgaacatcgg cgactacaac atgttgaccg agtgcccggc gggtacgacg
     1021 ctggtcgacc gcacgacgat ggcggcgggg atcacggtca agaccaaggt gtgccaaaag
     1081 acctgcgaga ccgacgccga ctgccgctgg aacgccttcg acagcgtgtg ggccgcctcg
     1141 ggcgagttcc gctgccaggt gacggcgaac tccaacgacg tcaagatctg cgccgacgcg
     1201 cagaactgag gtggggttga aaggacacac gccgccgcgg cttgacggct cgcggcggcg
     1261 ctggtgttct ggaggccatg gcgacctcgc tgatcccgag cacccggcgg ctgcgcaccc
     1321 tggcggccta cgcggcgatg ctggcggtcg cggcgggggt ctacctgttc atccgcgggc
     1381 gcggcgagca gctcgtcgcc ccggccgtgg acgtggtgcg cgaggctcac gtcgcgcact
     1441 cgagcacgct ggcgacggtg ttgctggcgc tggcggtgat catcgtgatg gcgcgcgggc
     1501 tcggccacct gtttcagcgg tggttcgggc aaccgccggt gatcggtgag atcgtcgcgg
     1561 gcctggtgct cgggccctcg gtgctcggcg cgatctcccc ggaggcggcc cagatgctgc
     1621 taccggccgc ggcggcgccg ttcctcggca tcctctccaa ggtcggcgtg gtgctgttca
     1681 tgttcctggt cggcctcgag ctcgacccga agatgctgcg cggcaacggc cacgcgacgc
     1741 tggcgatctc gcacgcgagc atcgtcgcgc cgttcctgct cggggcgacg ctggccctcg
     1801 gcctgtaccc gctgtactcg aactcgtcgg tcagcttcac cgtgttctcg ctgttcctcg
     1861 gggtgtcgct gtcggtgacg gcgttcccgg tgctggcgag gatcctgacc gaccgcaggg
     1921 tccagagcac gccgctcggg gtcaccgcgc tgacctgcgc ggcgatcgac gacgtcagcg
     1981 cgtggagcct gctggcgctg gtcgtcagcg tggcgagcgc gcaggcggcc ggcgtggcct
     2041 ggacggtcgg ggcggtcgtc gtctacctgc tggtcatgtt cgtcgcggtg cgcccgctgc
     2101 tggtccgctt cgccgcgagc gaggacgcca agaccgggcc gctggcgcag tcggcgatgg
     2161 cggtcgtgtt cgtggcgctg ctgctgtcgg cggtcgcgac cgaggcggcg ggcgtgcatg
     2221 cgctgttcgg cgccttcctg ctcgggacct tcttgccgca cgagggccgg ctcgccgagc
     2281 aggtgcgcct gcgactcgag gacgcggtgg tcgtgctgtt cctgccggcc ttcttcgcgt
     2341 tcaccggcat gcgcacgcag atcaacctcg tcgacggcgc gaccgactgg ctgatctgcg
     2401 ggcttatcat cctcatggcg acgctgggca agttcggcgg gtcgttcgcg gcggcgcggc
     2461 tgacggggct cggctggcgc gagagcgcgg cgctcggggt gctgatgaac acccgcggcc
     2521 tgatggagct gatcgtgctc aacgtcggcc tcgacatggg cgtgctgtcg ccgacgctgt
     2581 tcgcgatgct ggtgatcatg gccctggtga cgaccttcgc gacgacaccg gtcctcaaca
     2641 tggtgatggg cgagcgcggc ttcgcggacg cgcccgcgcg cgcgccggtc gatgtcggcc
     2701 accgcgcctg agggcggctt gcggcggccc acgcctggag tacaagagcg tatggacgcg
     2761 ctgcgagtcc gcttcttccc cgaggcactc gcccggaccc ccgggctcga cgcggcggcc
     2821 ctgaccgccg ccatccaggc ccacgtccgc gcccagcacg cgcgaccgga gcaggagccc
     2881 aacgagggcc cgcccgagga ggaggcggtc atccgcctcg tcaccccgca gcccgatggc
     2941 agcttccagg cccgcttcgg ctactggcgc aaccgcttcc cgagctcgcg ctacgacaag
     3001 tccgagtcgg ccgagggcga ggtgctgctc gacggagacg gcaccgtgcg ccgtgcggcg
     3061 ctcgggccgc tgcgcgaccg gttctgaagg ggccgcgagg gtctggagag gtcgggggtc
     3121 gggtcgaggc cggacgggcc gcgggtgctc gtcgcggcct tttcgtgtcg gactgtaaaa
     3181 cttcgcgcga agcggtaaaa ttggccgaat cctgcttccc gactgaattt ggtcccgtag
     3241 atgtgttctg tgtaattaaa taaactttgg gtcaaaagaa ttcatgtgca gaacagagct
     3301 gaccggaggt cgataaaaag gcgcaggaca tgcctaggcg accgggcaat gtcatcactc
     3361 gcaaaaagtg cggaagcgct cagccgaggc gacgcagcag gtccggggaa acgccgagcg
     3421 cggcgcagcg ttcggcgatc ggtgcgaggc gccagcggtc acggtcgacc ggggcgtagg
     3481 ggtggcggcg gtgggcccgg agcaggatgt tcttggcggt gtgctcgctg ctgacgaatt
     3541 cgacgaccgt gacctcgtag ccgcaggcgg cgaggatctc gacccgcaga ccgtcggtca
     3601 gcaaatcggc gtaggcgcga cgcaacaggc cgtgctcgag catcgccggg accggagcgg
     3661 tgcccgggtc gcgcttctcg agctgggcgg cgagctcgtg ctggcagcat gggacgcaca
     3721 gcaccgcggg cacgccgaga cggatcgcca gggccagggc ctcgtcggtc gcggtgtcgc
     3781 aggcgtgcag ggcgaggacg aggtcgggcg ggccgccgag cgccccggcg gcgctcgcct
     3841 cggcctcggc gatgctcgcg cgggcgaagc tcaggccgtc catgccgagg gtcgcggcgc
     3901 gctcgcggct gcgggcgacg acgtcctcgc ggacgtcgac gccgtgcagg cgcagcggga
     3961 ccgactcgcg gcgcaggacc tcggcgagca cgaagctgag gtaggcgttg ccgcaggcga
     4021 ggtcgagcgc gcgcaggggg gtctcggcgc tgcgcgggcg ggcggcggcg aggcgctccc
     4081 aggtcgggcg gcacagctcg gcgaggtggt tgacctgctt gtacttgcgg gcgtgggtcg
     4141 cggagatgct gccgtccgcg ttcatgatcc cgatcgcgcg cagcaggtcg gcgctggcgt
     4201 ccgggcgcag gacccggtcc ttgccgccca tgaccttgcg cgcggcggcc tcgtcgaggg
     4261 tgcgcacgct cgggcgcccg tgacgggcgt cgatctggac gcggcgcgtc tcgtcgatca
     4321 cgtaggtgtg tttgaccagg ccggcgttgg cggcgctgcg ggccgcggtg aacagcgccg
     4381 ccagctgctc gggcgtcggc ggcacaccgg cgcccgggcc cggcacgacc tgctcggcag
     4441 gcgcagaggg cggcggtgtc gtgccgtcgc cctgcggcgc ggcgaacacg gcccgcgtcc
     4501 agccgcggcg caggtgcggg gagatcgtgc gctctaggcg ttgcaggttg acttcacgga
     4561 gcacgcggcg gcagtagcaa agccgcgcgc acgcgacaag gtgcgaggcc acacgccgtg
     4621 cgccggcgtg cacggccgcg atctcgcggt cgtgatgcga tgcgcagcgc accgggcttg
     4681 cgagccggag cggtgctcca gtcaagcagc tgggcgcgaa gcggggcgtt cgtggacgct
     4741 tcatcggtgc aggtcggcga gcagcggggc gcgggcgtgg gcgaggtggg tctctggcca
     4801 gcgtcgcgga tgcgggaggc gaactcggtc gggcatcgag cggaccacgc cgcgcgggcc
     4861 ccgggtgact cgcggccctg ggcgatctcg cggcgcccgg gcggagggtt gcgagggcgg
     4921 ggggctttgg gcatcgtgcg cgccgtgtcc agcccgaaca tcctgctgct cgagagcatc
     4981 catgcgaccg cccacgagga gttcgcccgc cacggcgtcg tggtgcacac cgcgaccggg
     5041 gcgatgaacg agaagcagct ggtcgcggcg ctgcaggccc tgccgcagga cggcccggtg
     5101 atgctgggga tccgcagcaa gacgcaggtg acgccggcgg tgctcgaggc ggtgccgcag
     5161 ctgctggcga tcggcgcctt ttgtatcggc acggaccaga tcgcgctggc ggacgcgcgg
     5221 cggcgcggtg tggcggtctt caacgcgccg tttagcaaca cgcgctcggt cgccgagctg
     5281 gtgctcgggg agatcatcat gctgtcgcgg caggtgttcg cccgcagcgc cgcgtgccac
     5341 cgcggtctgt ggcacaagag cgccgacggc agccacgagg tccgcggcaa gacgctcggc
     5401 atcgtcggct acggacacat cggctcgcag ctgagcgtgc tcgccgaggc gctcggcatg
     5461 caggtgcgtt actacgacat cctgtccaag ctgccgctgg gcaacgcgca gccgtgcaac
     5521 agcctgcatg agctgctcgg gatcgcggac ttcgtgacgc tgcacgtgcc ggacacggag
     5581 ctgacgcgcg ggatgttcgg ggcggccgag atcgcggtga tgcgaccggg ggcgtacctg
     5641 atcaacgcgt cgcgcgggat ggtcgtcgac ctcgcggcgc tgcgcgacgg gctcgagcgc
     5701 ggtcgcctcg cgggcgcggc gatcgacgtg ttcccgagcg agccggccaa ggtcggcgac
     5761 gtgttcgaca gcccgctgcg cggcctggac tcggtgatcc tgacgccgca catcggcggg
     5821 tcgacgcagg aggcgcaggc caacatcggc cgcgaggtgg cgacctcgct ggcggcgtac
     5881 ctgacgagcg gctcgacgac gggctgcgtg acgctgccca acgtcgacgc ggcgtacatc
     5941 cgcggcggcg cgcggatcct gaacatccat cgtaacgtgc cgggcgtgct gtcggcggtg
     6001 aaccagctgc tggcggactc gggggtcaac atcaccgggc agagcctggc gacgatcgag
     6061 gcgatcgggc tcctgcacgt cgacgtgccg ctgcgcccgg acgacccgaa ggcgcatcag
     6121 ctcggcgccg cgatcgcggc cttgccgacc agcctgcgca ctcgcgtgat ctgacttcgg
     6181 cgatctctcg cagacggctg cactcgctgc gcgcccgacc gcgccgcggg cgctggaact
     6241 tgtcggcatc cggtggtcga cgcaattgcg tggacagagc cgaggctgcg agttccagga
     6301 tggcgacgat gtgggtgcgt gacgtggcgc gaaaatgtgt gggggcacgc atctacccca
     6361 cggcgctgcg gtgctgataa cctggagccg cgatgcgctt cacctggttt gcggcgggat
     6421 ttttggtcgt ggctctgagt cctacggcgt gcaagcggag cggggactcg aacaacccgg
     6481 gcggcgagtg cggcggtatg tgtggcaagg gcacgcgctg cgacgggaac atgtgcgtgg
     6541 tcgactactc gcaggacatc tgttcccagc cggaggaggt caacgagacg ccggtggtgc
     6601 cgatgaagcc gccgatcacg agctggggcg agtgttacca ggaccgcagc gagctgccga
     6661 agaagttcaa ggcggtcgac gacaagtcga tcccgcagtt tgaccccgac aagccgcggc
     6721 cggtcgacat gaacggcaac agcggcgacg agcagctcaa cgaggcgctg ctcaacgcga
     6781 acatgcggga catcgagtac gcgatcaacg agtgcctggc gaccgcggcc tgttatcagg
     6841 gctcgagctt gccggacggt cgcatcgact tcgtgttccg cctgctcggc accggcaaga
     6901 tcgacgcgat caccgtcacg gccccgaccg ggctgtcggt gttcggcatc gtcccgtgcg
     6961 cgcgcaaggc gatcgccgac cacaccttcc cgagctacga cgggccgccg atgacggtcc
     7021 gctacaacat ccagatcggc ggcggcgact agcttagccg cgggctcagc gggccggggt
     7081 ctcgtcgctg gggtccggct cgacgggggc ctcggcgtgg cgccggtaga cctgcgagat
     7141 gcgggccgcc gcggcgcggg cggccggcgc ggccttctcg acggcgtcct tgcggacctc
     7201 gaagggctcg ggcagcaagg tggaggatcc ctcggtcttg gacatgacga ctcgggacca
     7261 ctagcgtcgc agatcgccgc ggtggatcac aggaattgtc ggtgcggccc gcgggccttc
     7321 gcacgatcat tcgtcgtcgg tcggtgcggc gagcggcggc aggtcgaggc cggacagcgg
     7381 cgggcgcatg cgctcggccc ggcgcgtctg caaggtctcg atcacgcgct gcagggtcgg
     7441 gtcgtcgcgc cggcgcgcgt cgagcctggt gcccttggcg tagttgcagc gctcgcacgc
     7501 gaccgcgagg ttgcgtaggt catcggtgcc gccgtggtgg cgcgggatga tgtgctcgac
     7561 gctggcgcgg gtgcgcgggt gaccgtcgag gtcgatcacg tggcgcgcac cgcagtggat
     7621 gcacttgccg gtcaacaccg cggcgccctg ctgctcggcg aggtcgaagc tgcggtcggt
     7681 cgcggcgcac cacagcagca ggcggtgcgc gcggctcgat ggcttaacgc gccggggcat
     7741 gtccctgggg acaggtcccg gaggtggtcc gcgcgccggc cagctcggcc tgcaacaccg
     7801 gcgcgagggt cggaccggcg gcgacgaggt cgccgtggcg caggtcccag cggccgccgt
     7861 cgatgcgcgt cagctcgccg ccggcctcct ggacgagcag cgcgccggcg gcgacgtccc
     7921 acggctggag gtgatactcc cagtaggcgt cgaggcggcc ggcggcgacg taggcgaggt
     7981 cgagcgcggc gctgccggcc cggcggatgc agcggacctg cggcacgacg cggttgaact
     8041 cgagcaggtt gttggtcggg gtggtcgcgc gggtgtaggc gaagccggtg gcgacgagcg
     8101 cgaggcgcag cgcccgcgtg ggggtgacgc gcaggggctg ggggcggccg ttgcgggcgc
     8161 gggcgcgggc gctgtcgagg aaggcgccgc gtccgcggac ggcataaaag gtctcctcgc
     8221 gggtcgggtc gtggacgacg ccgagcagcg ggccctcgtg gtcgacgagc gcgagcgaga
     8281 cgctgaagtg ggggaagcgg tgggtgaagt tggtggtgcc gtcgagcggg tcgatgaccc
     8341 actggaggac gccgggcggg ggccgcgggt cggcgacgtc ggcgtcgccg gtgagccgcc
     8401 cggactcctc ggcgaggatc cagtgcgagg ggtgggcgcg agtgatgcgc tcggtcagcg
     8461 tcgcctcggc ggcgcggtcg gccgacgtga cgaggtcgat gtccgacgac ttggtcgaga
     8521 ccgtcagctc gccctcgaag tgttcgagca ggacgtcacc cgcagcgcgc gcggcggcga
     8581 gggcgaactc caaaagaccg gagtggagac cggggtcgag gccggggtcg tcgggcatac
     8641 ccgagcatag cgcaggtcga ggtcgcgcaa gctgtcccga gagtcaggcc agcagatcgg
     8701 acgctcaccg cgccggctca cacgtgctgg tgccaggacg gcgcggcccg gcgagatccc
     8761 ggcgcgcagg agcggcggat cgtttcagcc gcgggcgccg agcggggcgt cgctgctgcc
     8821 gcgcgcctgc ttgaacatgt tgttgacgtg gcgcagcacg tcgtcgcggt gacgggtcac
     8881 ctggacctcg tggtgaaaca cggcgtcgac gatcatatcc gggcggacga tgacggtgac
     8941 ccggcgggcg acgttcatca ccggccacag cacgtcgtac gagcggcaga tcttgtggtc
     9001 ggcgtccggc acgatcggga acggagcctt gagcgaggtc gcgaagtcac actgggtctt
     9061 ctcgtcgtcg gtgctgatgc cgacgaggtt ggccccggcg agcagcagct cgacgtagtt
     9121 gtcgcgaaac agcttggtct ccgcggtgca gcccggcgtg aaggccttgg ggaagaagta
     9181 gacgaccgtg cacagccccg cctgctgcga aagcacaaag cgctgcccgg tgctgctgac
     9241 ggcatcgatc tctggggcgc gctggcccgg gctcggcccg ctcatgacct cgttataacc
     9301 catgcgggcc ggcgcaggcg agggcgtgga ttggtttgcg gcggcagggc agcgcgaagt
     9361 agcctgcgag gcatgtccgc gctcgccagg gtcacacccg gatccgcagg cgcaggtcgg
     9421 ccgacgcgcg cccacgcggt cgcggcggcg atcgccgtgt gggtggtgct gagcctcggg
     9481 ctgccgctgt ggcagctcgg tcaggaggcg ctggcggtcg cgggcccggc ggcgccgacg
     9541 tgggtgttcg tgctcgcggg cgtgcgggcg ctggcgctgg cgctggcggt cgccggcatc
     9601 gcggtggtgt gcgcgtaccc ggtcgcccgc gcggtgccgt cgccggtgct gctgatgctg
     9661 ctgctggtct cgccgctggc gcgtgcgctc ggcgtgctcg gcctcggctt cgtgcccggg
     9721 gcgctcgcgg tgatcctggc ccaaagttca ggtgcgatcc cgctggcggc gctggtgttg
     9781 caagtgcggc tgcgcggacg tccggtcgcg ctgctcgagg cggcggcgga cctcggcgcg
     9841 gggccgtggc ggcggttccg ggcgatcgag cttccgctgc tcggcccggc gttcgcgctg
     9901 gcggcggggt ggtcggcgct ggtgtcgatc ggcgacgtga cgacgctcga gctcgccggt
     9961 ggcggcaagc tctacacgct cgcgctgctg ctgcgcgacg cggtgctgca cgaggccaac
    10021 ccgcggcggg ccctggcgat cgtcgcggtg atgctggcgg tcgcggtccc gtgcgcgctg
    10081 gcgatggctc gcgcgctggc cgggctcgcg ggcgagagca gcggcggaga ccgccggcca
    10141 ggacgcaacc tcaccgtgct cgggtggtgc ggcgtggcgc tgtcggccac gccgatgctc
    10201 gggctgctcg cgagtgtgac gcgagggacg ggcgagggga ccggggcgat gatgctgcgc
    10261 gggatgctgg cgcgcagcct ggtgttgacg ctcgcctgcg gggcgatcgc ggcgagcctg
    10321 ggcttcgcgc tggcgctggc gcggcggccg tcgcaggccg ggcgcgaccc ggtcgcggcg
    10381 ttgttgttgc tgccgctcgc gctgccgccg gtggtctacg gggcgttgat gttgccggcg
    10441 gggcggctgc tcgggctcgg gccggggccc gcgctgacgc tgctgggatt gctgccgatg
    10501 cgggtcgcgc tcgcgtatgc gggggcggtg ctcgcggtcg cggcggtgca acgcgaggtg
    10561 atcgacggcg cgcgcgatct cggggctgga cccatggcga ggctgctgcg gatctggtgg
    10621 cctctgctcg cgcccgcggg cgtcgctctc gcgctcgtcg gcttcgccca cgcgctcgcc
    10681 gacggcagcg tgcccgcgtt cacggcgggt cccggcggca gcacgctcgc ggtcgggatg
    10741 gcgatcgtcg cgcgtggtgg tgaggtcgac gtcgtgccgc gctgggcgct ggggctgtcg
    10801 ctggcgccag tgggggcggt gtggctcgcg gtctggacgg gagcccgggc ggcccgttga
    10861 tgcgacccac gagcgtcaca cggcgcacgc gccgaccgtc gcgcaggctg ccgggcgcag
    10921 gcgcactctg ttagtctacg cccgccgtgg tcaaagagct cgaccccctg gacgtgaccc
    10981 tgatgggcga caccgaggcg ccggcgaccc gcgaggtgac gccggtatcg gggctgtacg
    11041 cgggtcgcta tcagatcgag cgcgaggtcg ggcgcggcgg gatgggcacg gtgtatcgcg
    11101 cgctcgacgt gatggtcggc gatgtcgtgg cgctcaaggt gctcgacaag tcggtgaccg
    11161 gggcgtcgga tcagatggag tggtttcgcc gcgaggtccg cctggcgcga cggatcacgc
    11221 acccgaacgt cgcgcgcacg cacgacatgg gcgagcaggg cggcgcgcac tacatcacga
    11281 tggagtacgt cgagggcacg acgctgcagg acctgctgcg cgcgcgcgac gacgagggaa
    11341 gcgagggcag gcggcgccgg caggcgctcg agccggcccg ggcggcgcgc atcgctttgg
    11401 cggtcggtga ggggttggcg gccgcgcacg cggcgggcgt ggtgcatcgc gacctcaagc
    11461 cggcgaacgt gctgatcgat cgcagcggcc gcgtggtgtt gaccgacttc gggatcgccc
    11521 gggcgctcag cgacgagggg gccggggcgc gcacgatggg ggtggtcggc acgccgctgt
    11581 acatggcgcc ggagcagctc gcgggcacgc cggtcgacgt gcgcgccgac atctacgcgc
    11641 tcggcctgat tactttcgag atgctgacgg gggcgctgcc gttcgccggc gagacgccgc
    11701 tggcctcggc gctggcgcgc ctgacccagc cgccgcccga tcctcgcgga gcgagtccgg
    11761 gcctgccggc gccgctgtgc gagctggtgt tgcagtgcct ggcgcggacg gccgccgagc
    11821 ggcccgacag cgcgctcgcg gtcgccgagc gcctgcgggc ctggctgatc gccgcgggtg
    11881 agccgctggt cggcctgccg acggcgacga tgttcggcgc gggcgcgatg cccgaggccg
    11941 cgagcgccat ttcgtacagc aacctgacgc cgacgcagcc gcgggcggtg acgaccgcgg
    12001 tgccgggtgc atatacgcag gccgggctga cgcagaacgg cacacgcggc gggccgggca
    12061 tgactggcat gacggggatg acgggcatga cgcagcggcc gcagcggccg ctcgcgccgg
    12121 tgccgaccgc ggagcgggcc ctggcggtgc tgccgtttcg ctacatcggc ccgccggacc
    12181 agagctacct cggcgacgcg ttgacggacg agctgatcga cgtgctgtcg cggacgcgcg
    12241 ggctgcgggt gctcggcagc ggggcgacgg ccaagtatcg caacgagcgc gacccgcggg
    12301 cggtcggcaa ggacctcggg gtcgacgcgg tggtcgacgc gaccttgcag gcgagcccga
    12361 agcaggtgcg ggtggcggtg cgtttggtgg aggtcgccac cggcacgcag ctgtggagcg
    12421 agcgcttcga gagcggcctc gaggatctgt tcgagctgca ggaccggatc agcaagcgga
    12481 tcgcggaggc gctgcgggtc gagctgagca ccgcggcggg ctcgatggac gcgccggcgg
    12541 aggcgctctc gctgtatctg cgcggccgcc ggcagctgct ggcatttcac gtgaccgggc
    12601 ccgacggcgc gatcgagctg ctcgaggcgg cgatcgcgat ggcgccgcag ctcgacccgg
    12661 cgatcgcgtg tcacgcgatc gcgtgcctgc gggcgtggtt c
//
LOCUS       DASWNJ010000622         3491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_436400, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000622 DASWNJ010000000
VERSION     DASWNJ010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436400"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="VGB85_10440"
     CDS             <1..1013
                     /locus_tag="VGB85_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134492.1"
                     /translation="QGSALVPQARDFPGLGGERFTAIIATTKAKIPRSPSALAFTIAE
                     RDLIPEGITLDPAHRTFFVGSMYKRKIVAIGPDGAARDLVASAADDLHSPLGMRFDAT
                     RGSLWVASSALPTMQGFDEATDTGRAALHEYDAASGKLRARYPRDTQRPHLLNDLVLG
                     PRGELYLTDSEAGEVLQLTPGPGAEFEVLVPGGELYYPNGIALSDDASTLFVADFVHG
                     LTAVRLADRRRITLPHPRGASTRGIDGLYFRRGGLIGVQNGAGAGRIVRFDLAPALDR
                     VLGFEVLESGHPSFEIPTTGTLDGDTLVYIANSQMRSFDDGEIFPADRLRPVELLRLP
                     LK"
     gene            complement(1015..1584)
                     /locus_tag="VGB85_10445"
     CDS             complement(1015..1584)
                     /locus_tag="VGB85_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134493.1"
                     /translation="MAPVQYEVRTPPAYWAVVVLSCVVAAGSAALIVWMLAQGHMSWI
                     VLVFGGILTVLPAVYLGTTGEYRVRGVVRLGVGFIEVPGGPGEVLRFAVPGTVVATTR
                     VVVRYQMLGVPMADVKRGVVLELYEGTKRRRLSTLTLVDREYTEAMLADIAAVLRGAA
                     PRGPVAAEPRRPPADRLEQQLERELAALD"
     gene            complement(1637..>3491)
                     /locus_tag="VGB85_10450"
     CDS             complement(1637..>3491)
                     /locus_tag="VGB85_10450"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0134494.1"
                     /translation="AVKSPGPCPFCGADHDEATLRCPATDMVMPLAGRLLAGKFRLLS
                     EVGRGGMGAVWLARNIHVERDVALKLILPEVLKNPEIVARFRDEARVAARIGHPGICD
                     ILDFELTPIGPVIVMELLRGENLAQRIEREGRLPVPVALGIASQALVALEAAHRAGII
                     HRDLKPENLFLHQPGGGPMVVKLMDFGISKFTDRASGLTASGVIMGTPEYMAPEQIHG
                     AVNADARTDIWAIGAVLYRALTGEQPFTGQTVPEILIEIASGSPRPIGPEVPPALAGV
                     VLRCLRRAPAERWQSARELGEALQPFVTRSQDGAQLGSLPTLHSGVAPVVAELPPALR
                     INAATSSTRKGSRWPQRLVGLALLGACCYGAWWYAEQQRPQVARQGSGATTVAAAPGI
                     VPAKAEPPAATGAVAREVSTRRPGLLRLWGVLDDEPSGGTPGLDWVNAGLGHFAAALA
                     QPLPVEPVEPVPTSEASTGEDTDASGSDTGAPAGDGGAPVGTAGAPAGAAGEPAGAAG
                     EPVAEPPRLVSAGGFVTPSVVQELGSQHYAKKVCEAFNANKFLGIRGWKLANPAQAKL
                     FTGVAGLKPGSYWTTANYRGAGKVVSLPGGRDRSVSARKNAARALCVAPQG"
BASE COUNT          484 a         1422 c         1122 g          463 t
ORIGIN      
        1 cgcagggcag cgcgctcgtg ccccaggcac gcgacttccc cggcctcggc ggcgagcgct
       61 tcaccgcgat cattgcgacc acaaaggcga agatcccccg cagcccgagc gccctcgcct
      121 tcacgatcgc cgagcgcgac ctgatccccg agggcatcac cctcgacccc gcacaccgca
      181 ccttcttcgt cggcagcatg tacaaacgaa agatcgtggc gatcggcccc gacggcgccg
      241 cgcgggacct cgtcgccagc gccgcggacg acctccacag cccgctcggc atgcgcttcg
      301 acgccacccg cggctcgctg tgggtcgcca gctccgcgct gccgaccatg cagggttttg
      361 atgaggccac cgacaccggc cgcgccgccc tgcacgagta cgacgccgcc tccggcaagt
      421 tacgcgcccg ctacccgcgt gacacgcagc ggccgcacct gctcaacgac ctcgtgctcg
      481 gcccgcgcgg cgagctgtac ctgaccgaca gcgaggccgg cgaggtcctg cagctcacgc
      541 ccgggcccgg cgccgaattc gaggtgctcg tgccgggtgg cgagctctat tatcccaacg
      601 gcatcgccct gtccgacgac gcgagcaccc tcttcgtcgc cgacttcgtg cacggcctga
      661 ccgcggtccg cctcgccgac cgccggcgaa ttaccttgcc gcatccgcgc ggcgcctcga
      721 cccgcggcat cgacggcctg tacttccggc gcggcggcct gatcggcgtg caaaacggcg
      781 ccggtgccgg tcgcatcgtc cgcttcgacc tcgcccccgc gctcgaccgc gtgctcggct
      841 tcgaggtcct cgagtccggc caccccagct tcgagatccc gaccaccggc accctcgacg
      901 gcgatacgct ggtctacatc gccaacagtc aaatgcgcag cttcgacgac ggcgagatct
      961 tccccgccga ccgcctgcgc ccggtcgagc tgctgcgcct gcccctgaag tagatcagtc
     1021 cagcgcggcc agctcgcgct cgagctgctg ctccagccgg tccgccggcg gccttcgtgg
     1081 ctcggccgcg accggcccgc gcggcgccgc accgcgcagc accgccgcga tgtcggcgag
     1141 catcgcctcg gtatattcgc gatcgacgag cgtcagcgtc gacagcctgc gccgctttgt
     1201 cccctcgtac agctcgagca cgacgccccg cttcacgtcg gccatcggga ccccgagcat
     1261 ttgatagcgg acgaccaccc gcgtcgtcgc taccactgtc cccggcaccg cgaatcgcag
     1321 cacttccccg ggccccccgg gcacctcgat aaagccgacc ccgaggcgca ccactccgcg
     1381 cacccggtat tcgccggtgg tcccgagata cacggcaggc agcaccgtca ggatcccgcc
     1441 gaacaccagc acgatccacg acatgtgtcc ctgcgccagc atccacacga tcaacgcggc
     1501 gctacccgcc gcgacgacac acgacaatac gaccaccgcc cagtacgcgg gcggcgtgcg
     1561 gacctcgtac tggaccgggg ccatgccgcg agcgtacccg cgcccgcgcc gcaaagatac
     1621 cgcggcagcg atgcactcag ccctgcggcg cgacacacag cgcgcgcgcg gcgttcttgc
     1681 gcgccgacac cgaacgatct cggccgcccg gcagcgacac caccttgccc gcgccacgat
     1741 agttcgccgt cgtccagtag ctgcccggct tgaggcccgc gacgccggtg aacagcttcg
     1801 cctgcgccgg gttggcgagc ttccagccgc ggatcccgag gaacttgttg gcgttgaacg
     1861 cctcgcagac cttcttggcg tagtgctgac tgccgagctc ctgcacgacc gagggcgtga
     1921 cgaacccccc cgcggacacc aggcgcggcg gctcggccac cggctccccg gccgcgcccg
     1981 ccggctcccc ggcagcgccc gccggcgccc cggcagtgcc caccggcgcc ccgccgtccc
     2041 cggccggcgc ccccgtgtcg gagccactcg cgtccgtatc ctcgccggtg ctcgcctcgc
     2101 tcgtcggcac cggctcgacc ggctcgaccg gcagcggctg cgccagcgcg gccgcgaagt
     2161 ggccgaggcc cgcgttcacc cagtcgaggc ccggcgtgcc accggacggt tcatcgtcga
     2221 gcacgcccca caatctcagc agccccggtc tgcgcgtgct cacctcgcgg gcgaccgcgc
     2281 cggtggccgc cggtggctcc gccttcgccg gcacgatccc cggtgccgcg gcgaccgtcg
     2341 tcgcgccgct gccttgccgc gcgacctgcg gccgctgctg ctccgcgtac caccacgcgc
     2401 cgtagcaaca cgccccgagc agcgcgagcc cgaccagccg ctgtggccag cgcgagccct
     2461 tgcgggtcga gctggtcgcc gcgttgatgc gcagcgccgg cggcagctcg gcgacgaccg
     2521 gcgcgacccc gctgtgcagc gtcggcagcg agccgagctg cgccccgtcc tggctgcggg
     2581 tcacgaacgg ttgcagcgcc tcgccgagct cacgcgccga ctgccagcgc tcggcgggcg
     2641 cgcgtcgcag gcagcgcagg accaccccgg cgagcgccgg aggcacctcg ggcccgatcg
     2701 gccgcggcga gccgctggcg atctcgatca ggatctccgg caccgtctgg ccggtgaatg
     2761 gttgttcgcc ggtcagcgcc cggtagagca ccgccccgat cgcccagatg tccgtgcgcg
     2821 cgtcggcgtt gaccgccccg tggatctgct ccggggccat gtactcgggc gtgcccatga
     2881 tgacgccgct ggcggtcagg ccgctcgcgc ggtcggtgaa cttcgagatc ccgaagtcca
     2941 tcagcttgac gaccatcggc ccgccgccgg gctggtgcag gaacaggttc tccggcttga
     3001 ggtcgcggtg gatgatcccc gcgcggtggg ccgcctcgag ggcgacgagc gcctggctgg
     3061 cgatgccgag cgcgaccggc accggcagtc ggccctcgcg ttcgatccgc tgggccaggt
     3121 tctcgccgcg cagcagctcc atcacgatca ccgggccgat cggcgtcagc tcgaagtcga
     3181 ggatgtcgca gatccccggg tggccgatcc gggcggccac ccgcgcctcg tcccggaagc
     3241 gcgccacgat ctccgggttc ttgagcacct ccggcaagat cagcttgagc gccacgtcgc
     3301 gctcgacgtg gatgttgcgc gccagccaca ccgcccccat gccgccgcga ccgacctccg
     3361 agagcaggcg aaacttcccc gccagcaggc gccccgccag cggcatcacc atgtccgtcg
     3421 ccgggcaccg cagggtggcc tcgtcgtggt ccgccccgca aaacgggcac ggccccgggg
     3481 acttcactgc c
//
LOCUS       DASWNJ010000623         1580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_46947, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000623 DASWNJ010000000
VERSION     DASWNJ010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46947"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..965)
                     /locus_tag="VGB85_10455"
     CDS             complement(108..965)
                     /locus_tag="VGB85_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0134495.1"
                     /translation="MSLAPFRVISVREAGVALTALQPLAEAVFGAQGRSPGWFARKLA
                     REAIDPGRSFLALGPGDVRIGYLLISDDPLDRVAHSAGLGVLAEHRRRGVGSALIDRA
                     SAELVGAAPALQFLAEPPLRGFYERLGFTAQRTCHTLRSTGTGAVGLDLREHPPAAWT
                     LPGHAVAGWRPGTWSRTPSADAATVSLLDGGAWAHLSREGRAIVVQRLCVAGDPATTL
                     VADTHAALDELRGRLSRGTALLVVGCEAVSCVTASLLRHEHWQVAQTAVEMRRVIGEG
                     VDKHAGPAA"
     gene            complement(986..>1580)
                     /locus_tag="VGB85_10460"
     CDS             complement(986..>1580)
                     /locus_tag="VGB85_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY0134496.1"
                     /translation="ADILAYDGTLVPVGKDQLQHVEMAQDMATHFNEAFGPALVRPEP
                     LPAKAGVVPGVDGRKMSKSYGNTLPLFESGKKLRKLVGQVVTDSTPLGEPLPFETCNV
                     VALLRLFCDEAELTQIAAWYRDGRRDGAPFGYGHAKMLLADKIEQHFAPARIRRERLL
                     TPEGRGEVENILRRSAARARALAQLTLERCRKACGLR"
BASE COUNT          238 a          543 c          592 g          207 t
ORIGIN      
        1 tgccggcatc ctcgccgcga cagccagtga cagtgaagac ggagatcaac gctacgagag
       61 caggattaag cttgaacatg gggcacgctc cgggaaaggt cgcggcatca tgcggcgggt
      121 cccgcgtgct tgtccactcc ctcaccgatg acgcgccgca tctccacggc ggtctgtgcc
      181 acttgccagt gttcatgtcg cagcaatgaa gcggtgacgc aactcacggc ctcgcatcca
      241 accacgagca aggccgtgcc acgcgacaga cggccgcgaa gctcgtcgag ggcggcgtgc
      301 gtgtctgcga cgagcgtggt cgctggatcc ccggcgacgc acagtcgctg cacgacgatc
      361 gcacgaccct cacgcgagag atgagcccac gcgccaccat cgaggagtga gacggtcgcc
      421 gcgtcggcgc tgggggtgcg tgaccacgtg cccggtcgcc agccggcgac ggcgtgaccg
      481 ggcagcgtcc acgcggccgg cggatgctcg cgcaggtcga gcccgacggc gccggtgccc
      541 gtgctccgca aggtgtggca ggtgcgctgg gcggtgaagc cgaggcgctc gtagaagccg
      601 cgcagcggcg gctctgcgag gaattgcaag gccggcgccg cgccgacgag ctcggccgag
      661 gcgcggtcga tcagcgccga gccgacgccg cgccggcggt gctcggcgag cacgccgagg
      721 ccggcgctgt gggccacgcg gtcgagcggg tcgtcgctga tcagcagata gccgatccgg
      781 acatcacctg gcccgagggc caggaatgat cggcccgggt cgatcgcctc gcgcgcgagc
      841 ttgcgggcga accaccccgg cgaccggccc tgcgccccga acacggcctc ggccagcggc
      901 tgcagggcgg tcagcgccac gccggcctcg cggaccgaga tcacacgaaa gggcgcgagg
      961 gacatggccg ccgggacagg tcgcgtcagc gcaggccgca ggccttgcgg cagcgctcga
     1021 gggtcagctg ggcgagcgcg cgggcgcggg cggccgagcg gcgcaggatg ttctcgacct
     1081 cgccgcggcc ctcgggggtc agcaggcgct cgcggcggat cctcgcgggg gcgaagtgtt
     1141 gctcgatctt gtcggcgagc agcatcttgg cgtggccgta gccgaagggc gcgccgtcgc
     1201 ggcgaccgtc gcggtaccag gcggcgatct gcgtcagctc ggcctcgtcg cagaacagcc
     1261 gcagcagcgc gacgacgttg caggtctcga agggcagcgg ctcgccgagc ggggtcgagt
     1321 cggtgacgac ctggccgacc agcttgcgta gcttcttgcc ggactcgaac agcggcaagg
     1381 tgttgccgta gctcttgctc atcttgcgcc cgtcgacgcc cggcaccacg ccggccttgg
     1441 ccgggagcgg ctcggggcgc acgagcgccg gcccgaacgc ctcgttgaag tgcgtcgcca
     1501 tgtcctgcgc catctcgacg tgctgcagct ggtccttgcc gaccggcacg agcgtgccat
     1561 cgtacgcgag gatgtcggcg
//
LOCUS       DASWNJ010000624         2535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_152652, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000624 DASWNJ010000000
VERSION     DASWNJ010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152652"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VGB85_10465"
     CDS             <1..552
                     /locus_tag="VGB85_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134497.1"
                     /translation="LTPAIEDGADELARRGLSGLSGLSGLGIGIGAVIADPRLPARRL
                     MAHAHDLERSAKRICRPGHAKAVRSAFDFAILTAGTASIHDPTARGEADDRPIAVTQL
                     GPLKTAAQHLARIPTSQRVLLSEAAEYDAAEFANLLRYQVARSTEWQRWYDAGGHDWR
                     DPDIVVQRRPRPIHLDLARLLGV"
     gene            554..2353
                     /locus_tag="VGB85_10470"
     CDS             554..2353
                     /locus_tag="VGB85_10470"
                     /inference="COORDINATES: protein motif:HMM:NF015728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAMP superfamily CRISPR-associated protein"
                     /protein_id="HEY0134498.1"
                     /translation="MQFDLLVRVPAGSLLIGGHNSVPVGLHGVHASDRDGVPIVPSTA
                     LRGALRETLEGLVRGAAERSDPACAAGTGLPPGADATQRPQPCRLGDDGGPCLPCRLF
                     GGQRDGLGGDRHFSALVLGEARPTGEKPESMVRPGVAIRREARAAHEHRLFLRRVPMP
                     APDLEFRASGRLLDGQLRDALEAAVRATHHVGSGRSRGLARVELRLDWRPDDPVATPP
                     LPVGDVRVRVTLRTPAALGAPIADDNLRDTRREVPGSALRGAIGFALAERRNGEDPTM
                     ERLLAETDGADFGFLYPVDVSGADGISAPLPITATTCKYEGRAHILQDTLLDALLVAH
                     IDAPAAADRVHELTSVRCPHRDCGAPLRMCSGNRRRTQPLPTRTLTRVNIERTSGTAR
                     DGHLFTQVLLERGSTFEGTIRNVPEGTREHLAQALAAPLSLGRGRAHGWGLVDITTSA
                     APQIPTVSERGREFDAALKAALTRAQLSTERVGRLVPVTLLAPLIPETDDGGGALCAA
                     LAPARVFLRIRRFTRDGGWDQRGGKMQPAHAVTAGSVFVLELTDRAWQRDLARLADLE
                     RQGIGDRRCQGYGQVLCFDPFILQTAASRSIAR"
     gene            2350..>2535
                     /locus_tag="VGB85_10475"
     CDS             2350..>2535
                     /locus_tag="VGB85_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134499.1"
                     /translation="MTHHQELWALRGPLVRAAEASIKQIFNGPKQPDKAQLSHLVGVC
                     NAAACGEEIQLYLRYQAS"
BASE COUNT          364 a          979 c          807 g          385 t
ORIGIN      
        1 ctcacgcccg ccatcgagga tggtgcggat gaactcgcca ggcgcggcct ctccggcctc
       61 tccggcctct ccgggctcgg catcggtatt ggcgccgtga tcgctgaccc gcgcctaccc
      121 gctcgccgat tgatggctca cgcccacgac ctcgagcgct ccgccaaacg gatctgccgt
      181 ccgggccacg cgaaagcggt ccgttccgcc ttcgacttcg cgattctcac cgctggtacc
      241 gcatcgattc acgaccccac tgcccgcgga gaggcggatg accgaccaat cgcggtgaca
      301 cagctcgggc cgctcaagac cgccgcgcag cacctcgccc ggatccccac cagtcagcgc
      361 gtgctcctca gcgaggccgc agaatacgat gcagccgagt tcgccaactt gctgcgctac
      421 caggtcgccc gctcgaccga atggcagcgc tggtacgacg ctggcggcca cgactggcgc
      481 gaccctgaca tcgtcgtcca gcggcgccct cgtcccattc acctcgacct cgctcgtctg
      541 ctcggagtct aggatgcagt tcgacctcct cgtgcgcgta cctgccggca gcctgctcat
      601 cggcggccac aacagcgtcc ctgtgggcct tcatggcgtc cacgccagcg accgtgacgg
      661 cgtcccgatc gtcccctcca ccgccttgcg cggcgcgcta cgcgagacac tcgagggcct
      721 cgtccgcggc gccgccgagc gctccgaccc ggcgtgcgcc gcaggtacgg ggctgccgcc
      781 gggtgccgat gcgacgcaac ggccacagcc gtgtcgactc ggtgatgatg gcgggccgtg
      841 cctcccgtgc cgcctgttcg gcggccagcg cgacggcctc ggcggcgacc gtcacttctc
      901 cgctctggtc ctcggcgagg cgcggccgac aggtgagaag cccgagtcga tggtgcgtcc
      961 gggcgtcgcc atccgtcgcg aggctcgcgc cgcccacgag caccggctgt tcctgcggcg
     1021 cgtgcccatg ccggcgccgg acctcgagtt ccgcgccagt gggcgcctcc tcgacgggca
     1081 actccgcgac gccctcgagg ccgctgtgcg ggcgacccac catgtcggta gcggtcgcag
     1141 ccgcggtctc gcccgcgtcg agctacgcct cgactggcgc ccggacgatc cggtagcgac
     1201 cccgcccttg cccgtcggcg atgtccgcgt gcgggtcacg ctgcggaccc cggccgctct
     1261 cggtgcaccg atcgcagatg acaacttgcg agatactcgc cgcgaggtcc cgggcagcgc
     1321 cctgcgcggg gcgatcggct ttgctctggc agagcgccgc aacggtgagg atccgacgat
     1381 ggagcgccta ctcgccgaga ccgacggcgc agacttcggc ttcctgtatc cggtcgacgt
     1441 atcaggcgca gacggcatct ctgcgccgct gccgatcacc gctaccacgt gtaagtacga
     1501 ggggcgggcc cacatcctcc aggacacgct gctcgacgcg ttgctggtcg cgcatatcga
     1561 cgcgccagcc gcagccgacc gggtgcacga gctgacctcc gtccgctgtc cccatcgtga
     1621 ctgcggcgcc cccctgcgta tgtgcagcgg taaccggcgg cgcacgcagc cgttgccgac
     1681 ccgcacgctc acccgcgtca atatcgagcg tacgtcgggc acggcgcgcg acggtcactt
     1741 gttcacgcag gtgctcctcg agcgcggcag caccttcgag gggacgatcc gaaacgtccc
     1801 cgagggtacc cgtgaacacc tggcgcaggc cctcgccgcg ccgctctcgc tcggtcgcgg
     1861 gcgagcacac gggtggggtc tcgtagacat caccacctcc gccgcgccgc agatacccac
     1921 ggtcagcgag cgcggccggg aattcgacgc ggccctgaaa gcagcgctga cccgggcgca
     1981 gctctcgacc gagcgcgtcg gtcgcctcgt tccggtgacc ttgctcgctc cgctcatccc
     2041 ggagaccgac gacggcggcg gcgccctctg cgcggccctc gccccggccc gggtcttcct
     2101 acggatccgc cgtttcaccc gcgacggcgg ctgggaccaa cgtggcggca agatgcagcc
     2161 cgcgcacgca gtcactgcgg gcagtgtctt cgtgctcgag ctgacggatc gcgcgtggca
     2221 gcgagacctc gcccgcctcg ccgacctcga gcgccagggc atcggcgacc gccgctgtca
     2281 gggctacggc caggtcctct gcttcgaccc cttcattctc cagacggccg cttcacggag
     2341 tatcgctcga tgacgcatca ccaagaactc tgggctctgc ggggcccgct cgtccgcgcc
     2401 gccgaggcgt ccatcaaaca gatcttcaat ggaccgaaac aaccggacaa ggctcagctc
     2461 agccacctcg tcggcgtatg caacgctgcc gcctgcggcg aggagatcca actatatctc
     2521 cgctaccagg cctca
//
LOCUS       DASWNJ010000625         1662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_397247, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000625 DASWNJ010000000
VERSION     DASWNJ010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397247"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VGB85_10480"
     CDS             <1..505
                     /locus_tag="VGB85_10480"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY0134500.1"
                     /translation="VDHRAGVPDEDLHRHAAANIERADALILGRVTYEMMESAWRAPT
                     SNGTTPDSMDPFARTIDAARKYVVSSTLDRVDWNAELLRGDLGTAVLQLKQEPGKGLA
                     VGGVKLPLALAELGLIDEYEFVVHPTLAGHGPTLFAGLSKRIDLKLVSRLELRSGAMV
                     MRYEPRR"
     gene            complement(557..1027)
                     /locus_tag="VGB85_10485"
     CDS             complement(557..1027)
                     /locus_tag="VGB85_10485"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0134501.1"
                     /translation="MTIRSLLQDVSGELDGGAALRFVVADFRQVVGLDCSAAATLARL
                     QRRLQARGVALVCTGLTPAVERLLTIAGCELHGASAQVHADLDHGLEFCEERLLADAG
                     ASREEVRLADEFAALAGDASLLPRLLAFAAHVNLPGRARPPGERRCGPESAPRA"
     gene            1208..>1662
                     /locus_tag="VGB85_10490"
     CDS             1208..>1662
                     /locus_tag="VGB85_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134502.1"
                     /translation="MSACPGDETATLGTETGGTTLAELGTTTAGATGAAATTTTMPTT
                     GEPGTGTVASTGTDTHATTMIDTTGPVPACTDGDMCPDGQFCDLATHTCAVGCAHDDD
                     CAGGDDVHCDPETHACVGCLQDGDCAPGSLCVAHACAPGCSPTQGCPEGL"
BASE COUNT          265 a          538 c          647 g          212 t
ORIGIN      
        1 cgtcgatcat cgcgcgggcg tcccggacga ggacttgcat cggcacgccg ccgcgaacat
       61 cgagcgggcc gatgcgctca ttctgggccg ggtgacctac gaaatgatgg agtccgcgtg
      121 gcgggccccg acctcgaacg gaacgacgcc tgacagcatg gaccccttcg cccggacgat
      181 cgatgccgcc aggaagtacg tcgtgtcgag caccctggac cgggtagatt ggaacgcgga
      241 gctcctgcgg ggggacctgg ggacggcggt tttgcagctc aagcaggagc cgggcaaggg
      301 cctggccgtg ggcggcgtga agctcccgct ggcgctggca gagctgggat tgatcgacga
      361 gtacgaattc gtggtgcatc cgacgctggc gggccacggg ccgacgctgt tcgcggggct
      421 atcgaagcgt atcgacttaa aactcgtgag ccggctggag ctccgctcgg gggcgatggt
      481 gatgcggtac gagccgagga ggtagccatc gcgcccgcct ggcgcccaga aaggatgggt
      541 tcgacggacc gccgggtcat gcgcgcggcg ctgattcagg gccacatcgg cgctcaccag
      601 gcgggcgagc gcgcccgggc aggttgacgt gggcggcgaa cgccagcagg cgcggcagca
      661 gcgacgcgtc gccggcgagc gcggcgaact cgtcggcgag gcggacctcc tcgcgcgacg
      721 cgccggcgtc ggcgagcagc cgctcctcgc agaactcgag gccgtggtcg aggtcggcgt
      781 ggacctgcgc cgacgcgccg tggagctcgc agccggcgat cgtcagcagg cgctcgaccg
      841 cgggggtcag gccggtgcac acgagggcga cgccgcgggc ctggaggcgg cgctgcaggc
      901 gagccagcgt cgccgcggcc gagcagtcga ggccgacgac ctggcggaag tcggcgacga
      961 cgaagcgcag cgcggcgccg ccgtcgagct cgccgctgac gtcctggagc aggctccgaa
     1021 tcgtcaccca tgggctatca cggggcgggg gcccgcgaat ttcacgacaa tttcacggct
     1081 ccgcggcctc gggcggcgtc gtcaaatcgc gcgcatgagc ccgcgtcacc gcgtgatagt
     1141 ctgcgagacg aggcggagtg acatgcgaag tgatcgatgg atgatactgg tggcgtcgct
     1201 cgcgtggatg agcgcgtgtc ccggcgacga gactgcgacg ctggggacgg agacgggtgg
     1261 gacgaccctc gcggagctgg gcaccacgac cgcaggagcg acgggggcgg cggcgacgac
     1321 caccacgatg ccgacgacgg gagagcccgg gaccgggacg gtcgcctcga ccggtacgga
     1381 tacgcatgcg acgacgatga tcgacacgac tgggcccgtg ccggcttgca ccgacggcga
     1441 catgtgcccg gacggccagt tttgtgacct ggcgacgcac acctgcgcgg tcggatgcgc
     1501 ccacgacgac gactgcgcgg gaggagacga cgtccactgc gacccagaga cgcacgcctg
     1561 cgtcggttgt ctgcaggacg gcgactgcgc gccgggctcg ctgtgcgtcg cgcacgcgtg
     1621 cgctcccggc tgctcgccga cgcagggttg cccggagggc ct
//
LOCUS       DASWNJ010000626         7197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_47000, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000626 DASWNJ010000000
VERSION     DASWNJ010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7197
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47000"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VGB85_10495"
     CDS             complement(<1..332)
                     /locus_tag="VGB85_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134503.1"
                     /translation="MRTNHALKTFVIGALVLTGCPKDGEDTGATTKTTNQSGGAEAGE
                     SESTTGASAGTTTGATAPVTTTDPNPTTDVSVTEAQTSAPMTTASDDSTGCSFLGCDS
                     SGGGPVDN"
     gene            complement(431..1516)
                     /locus_tag="VGB85_10500"
     CDS             complement(431..1516)
                     /locus_tag="VGB85_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134504.1"
                     /translation="MSIVALHTQALVSPEAEVYLELLGRALQAARLNNSFPDRRQLQN
                     TLDTLRDALRHGVYGELYVDARAGLPNLASLTRVRTDQEVATASLARMGARASLEARR
                     DEAEVFARLAAKHAYFERLQKLELAPVDHHRVLLRRHDPATGTAAFRIELTKLDASGV
                     YLRLLIELTQVSSLWRRKVIDLDEDGETAAANEAFRSTVYRCADLDAETLFVRLHGIE
                     GVRVERVARGVVGPALFSLPAGEGVMVRRTAEAPDDALGRAWHQWSLGAGAAETERPE
                     LLVAFATDCAAGDIREEKSNDPLSPLLALRLAPAESARYRALRERYPFKVFKDRKFVA
                     TPGLKAMAQGVCDAAGTRNILYDLRLA"
     gene            complement(1537..2601)
                     /locus_tag="VGB85_10505"
     CDS             complement(1537..2601)
                     /locus_tag="VGB85_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134505.1"
                     /translation="MSSKVNLDVLNRQVAYSGEGISVKSLETEIAKEREVQDTKVDEL
                     KGLRERNVQIQGAMADELKKLRKFSDYLNGTSTKGGFLAGFKEVLSYIPGLKGLALSQ
                     RSIEELLKAQYHLSARRVKDAAEYVDTLKQSEQDLYKEIDRINVKIVEMAKNEREALG
                     YVLELKQVQEQLKAEEQAVEAGSVEQRDIQASADKVHAKLAEHSTNVLLYGSAEERYA
                     RLKDNTRKLAETIRNLANDIQQYTTAASIKLDMASAQIQAIGRAADASAVMLEMKRSL
                     DVMTESMNVTTQFVSDTQIFFRTNLDTLIKDLDTFDETTTTVLDENLKKSREVEEARI
                     KAAIEKATQRQQGAAPAPVG"
     gene            2829..5651
                     /locus_tag="VGB85_10510"
     CDS             2829..5651
                     /locus_tag="VGB85_10510"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEY0134506.1"
                     /translation="MDIESTLSGHNLAFLEALYEAYQRDPSSVDPQWVPVLRDLGLDR
                     GGSPGSGDAGAAATPTPTPTPVASPQSAQEVEQFTLQSRVNKLIESYRLHGHMAATVD
                     PLGRPRKAATPGLDPAYFGLGEEHMDRNFDPGLLMPGGNATLRQIWDRLKNTYCRNVG
                     VEYWHIQDLEQRTWLQARMEGCENKLTPPVAEQHRLLNMMAGIESVDKFLHSKFLGAK
                     RFSISGAESMIALLDCMIEEGAELGIGEIIFGMAHRGRLNVLMNIMGKSVADVFSEFE
                     KGDPWESLGSGDVKYHMGYFRQYTTEKGHEMYLALAFNPSHLEAISPVIQGRVRGKQD
                     AIGDNGGERVMGITIHGDAAFAGQGVVQETLNMARLRGYRVGGTIRLVINNQVGFTTD
                     PQDSRSTVYSSDAAHLLQVPVFHVNGDDVEANAYVARLAMAFRQRFKSDVVVDLVCYR
                     KFGHNEGDDPTFTQPLMYELIKNHPSVRTIYQRELIARGTITEPEANALDERWTKQYD
                     DALAQARSKSRKPRRAPMHGVWQAYRGGPDVETPDVPTAVDRATWDQISGRLTRWPED
                     FNLHPKLQRLVTEAKEMLAGNQPLNWQMGEMTAYASLLSAGINVRFSGQDSQRGTFSH
                     RHAVFTDIKTGRAWSPLYELNEGQGKFEIYNSPLSEYAVLAFEFGYSLIAPGSLVIWE
                     AQFGDFVNGAQIIIDNFLVSGEDKWNRLNGLVLMLPHGYEGQGPEHSSARLERFLQLA
                     AEDNIQVCNLTTPAQVFHVLRRQVLRPWRKPLILMSPKSLLRFRPSFSPVSDFIDGGF
                     QRAIDDVHADPEKVTRVMLSAGKVYYDLHEEREHLGADKVALVRVEQLYPFPTIQLAA
                     ILGRYPHARDLLWVQEEPKNMGGWTFVRPLLEDLIAQLHQVHGDRFSVPRYVGRAASA
                     SPATGSPDSHALERKLILEEAFANLA"
     gene            5687..7144
                     /gene="odhB"
                     /locus_tag="VGB85_10515"
     CDS             5687..7144
                     /gene="odhB"
                     /locus_tag="VGB85_10515"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF004309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEY0134507.1"
                     /translation="MPSSIKVPALGESITEAIIANWLQPVGARVEVDQPIVELETDKV
                     TVEVPSPVAGILLRHAADKGATVRVGDVIGEVDEAGTGAGAPAAGKTEGNATEANTTE
                     GSKPGANDAAGKDTGGISGSPASNTQKEETAAKQDAANAQAPKPETSAIPAPAMPSAR
                     AEAARSGVDLESVEGTGRGGRILKEDVQRAAQPQQQAQPAQAQQPQPAQAQQAQPAQA
                     QQAAPAKQAPAQQAQPAKPAAAPAQAKPATTSTPAGQEEIVAMSPLRRRVAERLVQAQ
                     STAAILTTFNEVDMSGIYKLRAAYKQSFVDKHGVKLGFMSFFVKAAVSALKAFPAVNA
                     EIRGDNIVYKHFWDIGVAVGGGKGLVVPVVRGADKLSFAEVEKEIGRLGTRARDNKLT
                     LDELSGGTFTISNGGVYGSMLSTPILNPPQSGILGLHNVVERPVAVNGAVEIRPIMYL
                     ALSYDHRLVDGREAVQFLVHIKERIEAPERLMLEV"
BASE COUNT         1122 a         2679 c         2302 g         1094 t
ORIGIN      
        1 tcgttgtcga ccgggccgcc accggaggaa tcgcagccga ggaagctgca gccggtgctg
       61 tcgtcgctgg ccgtcgtcat cggcgcgctg gtctgcgcct ccgtgacgct gacgtcggtc
      121 gtcgggttcg ggtcggtcgt cgtgaccggc gccgtcgccc ccgtggtggt gccagcgctg
      181 gcgccggtcg tcgactcgct ctcgcccgcc tcggctcccc cggattgatt ggtcgtcttc
      241 gtggtggcgc cggtgtcctc gccgtccttg gggcagcccg tcagcacgag ggcgccgatg
      301 acaaaagtct tcaaggcatg attagttcgc atcgcttcgt ctcctgctcg acggcggccg
      361 gcatgaccac cctactcgcg catcttcgcg caaagcgcga cgggacgcaa acaccagcgc
      421 aagggacgac tcaggcgagc cgcaggtcgt agagaatgtt tcgcgtcccc gcggcatcac
      481 agaccccctg ggccatcgcc ttgaggccgg gcgtcgccac gaatttgcgg tccttgaaca
      541 ccttgaaggg gtagcgctcg cgcagggcgc ggtaccgcgc ggactccgcg ggggccaggc
      601 gcagggccag cagcggcgac agcgggtcgt tgctcttctc ctcgcggatg tccccggccg
      661 cgcagtcggt cgcgaaggcg acgagcaatt cgggccgctc cgtctcggcc gcgccggcgc
      721 cgagcgacca ctggtgccag gcgcggccga gcgcgtcgtc gggggcctcg gcggtgcgcc
      781 gcaccatcac gccctcgccg gcgggcagcg agaacagggc cgggccgacg accccccgcg
      841 cgacccgctc gacccgcacg ccttcgatcc cgtgcagccg gacgaacaac gtctccgcgt
      901 cgaggtccgc gcagcggtac accgtgctgc gaaacgcctc gttggcggcc gccgtctcgc
      961 cgtcctcgtc gaggtcgatc accttgcgcc gccacagcga cgacacctgc gtcagctcga
     1021 tcagcaggcg cagatacacc cccgacgcgt cgagcttcgt cagctcgatg cgaaacgccg
     1081 ccgtgccggt cgccgggtcg tggcggcgca gcagcacccg gtggtggtcg accggcgcca
     1141 gctcgagctt ttgcaggcgc tcgaagtacg cgtgcttcgc cgccagcctg gcgaacacct
     1201 ccgcctcgtc gcgccgggcc tccagcgagg cgcgcgcgcc catacgggcc agcgacgccg
     1261 tcgcgacctc ctggtccgtg cgcacgcgcg tcaggctcgc caggttcggc agcccggcgc
     1321 gcgcgtcgac gtagagctcg ccgtagaccc cgtggcgcag cgcgtcgcgc agcgtgtcga
     1381 gggtgttctg cagctggcgg cggtcgggga agctgttgtt gagccgggcc gcctgcaagg
     1441 cccggccgag cagctcgagg tagacctcgg cctcgggcga gacgagggcc tgcgtgtgca
     1501 gggcgacgat cgacacgggg ctccctccgg ggtcgctcag ccgaccggcg ccggcgcggc
     1561 gccttgctgg cgctgggtcg ccttctcgat cgccgccttg atgcgcgcct cctcgacctc
     1621 gcgcgacttc ttgaggttct cgtcgagcac ggtggtcgtc gtctcgtcga aggtgtcgag
     1681 gtccttgatc agcgtgtcga ggttggtgcg aaagaagatc tgcgtgtcgc tgacgaactg
     1741 cgtcgtcacg ttcatcgact cggtcatcac gtcgaggctg cgcttcatct cgagcatcac
     1801 ggccgaggcg tcggctgcgc gcccgatcgc ctggatctgc gccgaggcca tgtcgagctt
     1861 gatcgacgcc gccgtcgtgt actgctggat gtcgttggcg aggttgcgga tcgtctccgc
     1921 cagcttgcgc gtgttgtcct tgaggcgggc gtagcgctcc tcggccgagc cgtacagcag
     1981 cacgttggtc gagtgctccg cgagcttggc gtggaccttg tcggcgctgg cctggatgtc
     2041 gcgctgctcg acgctgccgg cctcgaccgc ctgctcctcc gccttcagct gctcctggac
     2101 ctgcttcagc tcgagcacgt agccgagcgc ctcgcgctcg ttcttcgcca tctcgacgat
     2161 cttgacgttg attcggtcga tctccttgta gagatcttgc tcgctctgct tgagggtgtc
     2221 gacgtactcg gcggcgtcct tcacgcggcg ggcgctgagg tgatactgcg ccttgagcag
     2281 ctcctcgatc gagcgctggc tcagcgccag gcccttgagg cccgggatgt agctcaggac
     2341 ctccttgaag cccgccaaaa acccgccctt ggtgctcgtg ccgttgagat aatcgctgaa
     2401 cttgcgcagc ttcttgagct cgtcggccat cgccccctgg atctgcacgt tgcgctcgcg
     2461 cagacccttg agctcgtcga ccttggtgtc ctggacctcc cgctccttgg cgatctcggt
     2521 ctccagcgac ttgacggaga ttccctcccc ggagtaggcg acctggcggt tgagaacatc
     2581 gaggttgacc ttggagctca tcgtggggcg aaggttgaca caattccgcc gcggcttctc
     2641 ccctcttttt gcccgctggc accggccagt cccgccccgc gcgcccctgc acgccctccg
     2701 tccgagggcg ccgtagggcc cgacgctcgg cctttcacgg gtggcgcaga tcccgcgagc
     2761 gagcctgtcg gtgacgtgcc gcgcgaacgt tgtcaccccc cggtaaacga gtagactgcg
     2821 gcccgaccat ggatatcgag tccacgctca gcgggcacaa cctcgcgttc ctcgaagccc
     2881 tctacgaggc ctaccagcgc gatccgtcgt ccgtcgaccc gcagtgggtc cccgtgctcc
     2941 gcgacctcgg cctcgaccgc ggtggttcgc ctggttccgg cgacgcaggc gccgcggcaa
     3001 ccccgacgcc gacaccgacg ccggtcgcca gcccgcagag cgcccaggag gtcgagcagt
     3061 tcacgctgca gtcgcgcgtc aacaagctga tcgagagcta tcgcctgcac ggtcacatgg
     3121 cggcgacggt cgacccgctc ggtcgcccgc gcaaggccgc gacgccgggc ctcgaccccg
     3181 cgtacttcgg cctcggcgag gagcacatgg atcgcaactt cgatccgggc ctgctgatgc
     3241 cgggcggcaa cgcgacgctg cgtcagatct gggaccgcct caagaacacc tactgccgca
     3301 acgtcggcgt cgagtactgg cacatccagg acctcgagca gcgcacgtgg ctgcaggcgc
     3361 gcatggaggg ctgcgagaac aagctgaccc cgccggtcgc cgagcagcac cgcctgctca
     3421 acatgatggc gggcatcgag agcgtcgata aattcctgca cagcaagttc ctcggcgcca
     3481 agcgcttctc gatctcgggc gccgagtcga tgatcgccct gctcgactgc atgatcgagg
     3541 agggcgccga gctcggcatc ggcgagatca tcttcggcat ggcccaccgc ggccgcctca
     3601 acgtgctcat gaacatcatg ggcaagtcgg tcgccgacgt gttctccgag ttcgagaagg
     3661 gcgacccgtg ggagagcctc ggctcgggcg acgtgaagta ccacatgggc tacttccgcc
     3721 agtacacgac cgagaagggc cacgagatgt acctggcgct ggccttcaac cccagccacc
     3781 tcgaggcgat ctcgccggtc atccagggcc gcgtgcgcgg caaacaggac gcgatcggcg
     3841 acaacggcgg cgagcgggtc atgggcatca cgatccacgg cgacgccgcg ttcgccggcc
     3901 agggcgtcgt gcaagagacc ctcaacatgg cgcgcctgcg cggctatcgc gtcggcggca
     3961 cgatccgcct cgtcatcaac aatcaggtcg gcttcaccac cgacccgcag gactcgcgca
     4021 gcaccgtcta ctcgagcgac gccgcccacc tgctgcaggt ccccgtgttc cacgtcaacg
     4081 gcgacgacgt cgaggccaac gcctacgtcg cgcgcctggc gatggcgttt cgccagcgct
     4141 tcaagagcga cgtcgtcgtc gacctcgttt gttaccgcaa gttcggccac aacgagggcg
     4201 acgacccgac cttcacccag ccgttgatgt acgagctgat caagaaccac ccctcggtgc
     4261 gcacgattta tcaacgcgag ctgatcgccc gcggcacgat caccgagccg gaggccaacg
     4321 ccctcgacga gcgctggacc aagcaatacg acgacgcgct cgcgcaggcg cgcagtaaat
     4381 cgcgtaagcc ccggcgcgcc ccgatgcacg gcgtgtggca ggcctaccgc ggcggccccg
     4441 acgtcgagac ccccgacgtg ccgaccgccg tcgaccgcgc cacctgggac cagatctccg
     4501 gccgtctgac ccgctggccc gaggacttca acctccatcc caagctccag cgcctcgtca
     4561 ccgaggccaa ggagatgctc gccggcaatc agccgctcaa ctggcagatg ggcgagatga
     4621 ccgcctacgc gtcgctgctg agcgccggca tcaacgtgcg ctttagcggc caggacagcc
     4681 agcgcggcac cttcagccac cgccacgccg tcttcaccga catcaagacc ggccgcgcct
     4741 ggtcgccgct gtacgagctc aacgagggcc agggcaagtt cgagatctac aacagcccgc
     4801 tcagcgagta cgccgtgctc gccttcgagt tcggctacag cctgatcgcc cccggctcgc
     4861 tcgtgatctg ggaggcccag ttcggcgact tcgtcaacgg cgcgcagatc atcatcgaca
     4921 acttcctcgt gtccggcgaa gacaagtgga accgcctcaa cggcctcgtg ctgatgctcc
     4981 cgcacggcta cgagggccag ggccccgagc actcgagcgc ccgcctcgag cgcttcctcc
     5041 agctcgccgc cgaggacaac atccaggtct gcaacctgac gacgccggcc caggtgttcc
     5101 acgtgctgcg ccgtcaggtc ctgcgcccgt ggcgtaagcc gctgatcctg atgagcccca
     5161 agagcctgct gcgctttcgc ccgtcgttct cgccggtctc cgacttcatc gacggcgggt
     5221 tccagcgcgc catcgacgac gtgcacgccg accccgagaa ggtcacccgc gtcatgctca
     5281 gcgccggcaa ggtctattac gacctccatg aggagcgcga gcacctcggc gccgacaagg
     5341 tcgcgctcgt ccgcgtcgag cagctctacc ccttccccac gatccagctc gccgcgatcc
     5401 tcgggcgcta cccccacgcg cgcgacctgc tgtgggtgca agaggagccc aagaacatgg
     5461 gcggctggac cttcgtgcgg ccgctgctcg aggacctgat cgcccagctg catcaggtcc
     5521 acggcgaccg cttctcggtc ccccgctacg tcggccgggc cgccagcgcc agcccggcca
     5581 ccggctcccc cgacagccac gccctcgagc gcaagctgat cctcgaggaa gccttcgcca
     5641 acctcgcctg accgccctcc ctccgcaccc cgaccgcgag accaccatgc ccagctcgat
     5701 caaagtccct gccctcggcg agtccatcac cgaagccatc atcgccaact ggctgcaacc
     5761 cgtcggcgcc cgcgtcgagg tcgaccagcc gatcgtcgag ctcgagaccg acaaggtcac
     5821 cgtcgaggtc ccctcgcccg tcgccggcat cctcctccgg cacgccgccg acaagggcgc
     5881 caccgtgcgc gtcggcgacg tcatcggcga ggtcgacgag gccggtaccg gcgctggcgc
     5941 gcccgcggcc ggcaagaccg agggcaatgc gaccgaggcc aacacgaccg agggcagcaa
     6001 gcccggcgcg aacgatgccg ccggcaagga caccggcggc atctccggct cgcccgcctc
     6061 gaacacgcaa aaagaggaga ccgccgccaa gcaggacgcc gcgaacgccc aggccccgaa
     6121 gcccgagact tccgcgatcc ccgcccccgc gatgccctcg gcccgcgccg aggcggcccg
     6181 cagcggcgtc gacctcgaga gcgtcgaggg caccggccgc ggcggtcgca tcctgaagga
     6241 ggacgtccag cgcgccgccc agccgcagca gcaggcccag cccgcgcagg cccagcagcc
     6301 ccagcccgcg caggcccagc aggcccagcc cgcgcaggcc cagcaggccg cgcccgccaa
     6361 gcaagcgccc gcgcagcagg cccagcccgc caagccggcc gccgcccccg cgcaggccaa
     6421 gcccgcgacc acctcgacgc ccgccggcca ggaggagatc gtggcgatga gcccgctgcg
     6481 ccgccgcgtc gccgagcgtc tggtccaggc gcagtcgacc gccgcgatcc tgacgacctt
     6541 caacgaggtc gacatgtccg gcatttataa gttgcgcgcc gcctacaagc agagcttcgt
     6601 cgacaagcac ggcgtcaagc tcggcttcat gtcgttcttc gtgaaggccg ccgtctcggc
     6661 gctcaaggcc ttcccggcgg tcaacgccga gatccgcggc gacaacatcg tctacaagca
     6721 cttctgggac atcggcgtcg ccgtcggcgg cggcaagggc ctcgtcgtcc ccgtcgtgcg
     6781 cggcgcggac aagctcagct tcgccgaggt cgagaaggag atcggtcgcc tcggcacccg
     6841 cgcccgcgac aacaagctca ccctcgacga gctcagcggc ggcaccttca cgatcagcaa
     6901 cggcggcgtg tacggctcga tgctctcgac gccgatcctc aatccgccgc agtccggcat
     6961 cctcggcctg cacaacgtcg tcgagcgccc ggtcgcggtc aacggcgccg tcgagatccg
     7021 cccgatcatg tacctcgcgc tcagctacga ccaccgcctg gtcgacggcc gcgaggccgt
     7081 gcagttcctg gtccacatca aggagcgcat cgaggcccct gagcgcctca tgctggaggt
     7141 ttaaagagcg atgcccgaga acgctgacag cttcgatctc gtcgtcatcg gcgccgg
//
LOCUS       DASWNJ010000627         3879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_541978, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000627 DASWNJ010000000
VERSION     DASWNJ010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541978"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..900
                     /locus_tag="VGB85_10520"
     CDS             55..900
                     /locus_tag="VGB85_10520"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY0134508.1"
                     /translation="MTIADPDTWILGLDLGARGRGAVRVADWLAGPDRTIGVHVLEHW
                     TRPYIRPNAISAVHEAVRRIGDEFHVTPPARISVLEAASAEEGLAEAARGAAGLVIGR
                     VAGSRQDTPFGLGRVARRLLRSLPGPVVVVPRDLEAVAPGPVLLATDLDESSDAAAAF
                     ARALAGKHGRELVVVHVGERRHSDLIDELEPSWLAAREAYRDELAAALARWMARHDLA
                     QAPRRVVYGSTVDELVTLAATIDAAMLVVGSRRLGAAGRVFLGSTASALAGRAACPVA
                     VIPPA"
     gene            complement(905..1966)
                     /locus_tag="VGB85_10525"
     CDS             complement(905..1966)
                     /locus_tag="VGB85_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134509.1"
                     /translation="MAVRGRAAAALVQRTLARRCPGLAPVRFEPSDGTDEYGATWLGA
                     QPLGTTALILHKVTSGNGVYAEYDVDATRYDLVACTATTLRIDYADEALTGSVDEMPP
                     LSLLRQVVGPAHDRGDPAHAYATLLATTTGACVPEDPPDFTDDAPQAGAPGLEVLPLP
                     FGEQPWFPGPPRPQARVWREVQDGAQVIVPPKRLRRLRVVDGVEVFGADLPGRNGGLA
                     LALYDPRADRHRWLLFTRACLYGTSVYWLAEGSGLAVGYAASGHPVYWQEGRDGLFAI
                     DLIHARAYRLLLDGAAMLWEPPGSPEGGDHSEPAVQPDARQIGRLRKDATVLRSRCGT
                     EISLSSIRAAIDAGVATIP"
     gene            complement(2280..3608)
                     /locus_tag="VGB85_10530"
     CDS             complement(2280..3608)
                     /locus_tag="VGB85_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134510.1"
                     /translation="MLLAGRLLLLSLAVVTVACAGRKPAVSDQATPAVVSLDACAERY
                     VKLGLALGQHDANYVDAYYGPPAWAAQAKATSVSLAELESQAAALARELAAVPVPADP
                     LVALRRGYLLRQSQALAARVRLLRGEVLAFDDESEALYDARAPSHDDAHFMALQAELE
                     RLLPGPGPLADRVERFRLEFVVPPDRLDVVMRTALAEARRRTLLRVDLPANERVDLEL
                     VTGKTWGGYNWYRGDARSLVQINTDLPIFIGRAIDLAAHEGYPGHHVYNVLLEQHLMR
                     GRGWLEFSLYALYSPQSLIAEGSATYGVEVAFPDAPEYLRDVLFPLAGLDPRRAELYA
                     SVEELQKRLGYAQNEAARGFIDGRLTRAEASAWLARHGLVSLERADKLMSNVEVTRTY
                     VINYNLGRDLVADWIERQGGTAAAPDRRWQLFLELLSSPRLPGDLRRPGA"
     gene            complement(3663..3800)
                     /locus_tag="VGB85_10535"
     CDS             complement(3663..3800)
                     /locus_tag="VGB85_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134511.1"
                     /translation="MYLTVFMVPPSTLLTLLACWMTGAAIILGLFMLNRRRLLREHAS
                     E"
BASE COUNT          545 a         1407 c         1424 g          503 t
ORIGIN      
        1 cagcgcatat tgcaatggtg cctcgttcgt cgtcgacgga ggcatgttga ccgcatgacg
       61 atcgccgacc ccgacacctg gatcctcggg ctcgacctcg gcgcgcgcgg ccgcggggcg
      121 gtccgcgtcg ccgactggct cgccggcccc gaccgtacga tcggcgtgca cgtgctcgag
      181 cactggaccc gcccctatat ccgacccaac gcgatctcgg cggtgcacga ggccgtacgg
      241 cggatcggcg acgagtttca tgtcaccccg cccgcccgga tatcggtgct cgaggccgcc
      301 tcggccgagg agggcctcgc ggaggcggcc cgcggcgccg ccgggctggt gatcgggcgc
      361 gtcgccgggt cccgccagga caccccgttc gggctcggcc gcgtcgctcg ccgcctcctg
      421 cgatcgctgc ccggcccggt cgtcgtggtc ccgcgcgacc tcgaggcggt cgccccgggg
      481 ccggtcctgc tcgccaccga cctcgacgag tcgagcgacg ccgccgccgc gttcgcccgc
      541 gcgctggccg gcaagcacgg ccgcgagctc gtggtcgtgc acgtcggcga gcgacgccac
      601 agcgacctca tcgacgagct cgagccgagc tggctcgccg cccgcgaggc ctaccgcgac
      661 gagctcgccg ccgcgctcgc gcgctggatg gcccgccatg acctcgcgca ggcgccccgg
      721 cgcgtcgtgt acgggagcac cgtcgacgag ctcgtcaccc tcgccgccac catcgacgcg
      781 gccatgctcg tcgttggttc gcggcgcctc ggcgcggcag gccgcgtatt cctcggcagc
      841 accgcctcgg ccctcgccgg ccgggccgcc tgtccggtcg ccgtcattcc cccggcctga
      901 ggggtcacgg tatggtggcg acaccggcgt cgatcgccgc gcggatcgag gacagcgata
      961 tctcggtccc gcagcgactg cgcaggaccg tcgcgtcctt gcgtaggcga ccgatctggc
     1021 gcgcgtccgg ctgcaccgcc ggttcactgt gatcaccgcc ctccggcgac ccggggggct
     1081 cccacagcat cgcggcgccg tcgagcagca gccggtacgc gcgggcgtgg atgaggtcga
     1141 tcgcgaacag gccgtcgcgg ccctcctgcc agtacaccgg gtggcccgac gcggcgtagc
     1201 cgacggcgag gcccgagccc tccgcgagcc agtacaccga ggtgccgtac aggcaggcgc
     1261 gcgtgaacag cagccagcgg tggcgatcgg cgcgcgggtc atacagcgcg agcgcgagac
     1321 caccattgcg accgggcagg tcggcgccga agacctcgac gccgtcgacc acgcgcaatc
     1381 gtcggaggcg cttcggcggc acgatcacct gcgcgccgtc ctgcacctcg cgccacaccc
     1441 gggcctgcgg gcgcggcggg ccggggaacc acggctgctc gccgaagggc aatggcagca
     1501 cctcgagacc gggggcgccg gcttgcgggg catcgtcggt gaagtcaggc ggatcctcgg
     1561 gcacacacgc cccggtcgtc gtcgccagca gggtcgcata ggcatgcgcc ggatcgccgc
     1621 ggtcgtgggc gggcccgacc acctgccgca gcaaggacag cggtggcatc tcgtccacgc
     1681 tcccggtcag ggcctcgtcg gcataatcga tccgcagcgt cgtcgcggtg caggcgacga
     1741 gatcgtatcg agtcgcgtcg acgtcgtact cggcgtatac gccgttgccg ctggtgacct
     1801 tgtgaaggat caacgcggtg gtcccgagcg gctgcgcgcc gagccaggtc gcgccgtact
     1861 cgtcggtgcc gtccgagggt tcgaatcgca ccggcgcgag cccggggcag cgccgggcga
     1921 gcgtgcgctg cacgagcgcg gccgcggccc gcccgcgcac cgccacccgc ggctcctcga
     1981 ccacgccccg cggccggctg cgcgataccg gaggctgcca ggtcgacgcg atcgctatcg
     2041 tcggacatgc cggtgatatc ggtgacaccg gtgatactgg tgctatcggt gctatcggcg
     2101 atatcggtga cactggcgtc gcgggcggct cgggggtgac gagggcggcg tcgatcacag
     2161 ccggcgtcga cggcggtagc ggggtcgcgg ggaccggcgt gcacgcgagc aggaccgccg
     2221 gccagagcag ccggcgcggc gaggtgacga tggcccgcat caccaacagt atcgcacggt
     2281 cacgcgccgg gccggcgcag gtcgccgggc aggcgcggcg aggacagcag ttccaggaac
     2341 agctgccagc gccgatcggg cgccgccgcg gtgccgccct gccgctcgat ccagtcggcg
     2401 acgaggtcac ggccgaggtt gtagttgatc acgtaggtgc gggtgacctc gacgttgctc
     2461 atcagcttgt cggcgcgctc gagcgatacc agcccgtggc gcgcgagcca ggcgctcgcc
     2521 tcggcgcggg tgaggcggcc gtcgatgaag ccgcgcgcgg cctcgttctg cgcgtagccg
     2581 aggcgctttt gcagctcctc gaccgaggca tacagctcgg cgcgccgcgg gtcgaggccc
     2641 gccagcggga acagcacgtc gcgcagatat tcgggggcgt cggggaacgc gacctcgacc
     2701 ccataggtcg cgctgccctc ggcgatcagc gactgcggtg aatacagggc atacaatgaa
     2761 aactcgagcc agccgcgccc tcgcatcagg tgctgctcga gcagcacatt atagacgtgg
     2821 tggccggggt agccctcgtg ggccgcgagg tcgatcgccc ggccgatgaa gatcggcagg
     2881 tcggtgttga tctgcacgag gctgcgcgcg tcgccccggt accagttgta gccgccccag
     2941 gtcttgccgg tgacgagctc gaggtcgacc cgctcgttcg ccggcagatc gacccgcaaa
     3001 agcgtgcggc ggcgggcctc cgcgagcgcg gtgcgcatca cgacgtcgag ccggtccggt
     3061 ggcaccacga actcgaggcg aaagcgctcg acccggtccg ccagcgggcc cggacccggc
     3121 agcaggcgct ccagctcggc ctgcagggcc atgaagtgcg cgtcgtcgtg gctcggggcg
     3181 cgcgcgtcgt acagcgcctc gctctcgtcg tcgaacgcca gcacctcgcc gcgcagcagc
     3241 cggacccgcg ccgcgagggc ctgcgactga cgcagcagat aaccacggcg cagcgccacc
     3301 agcgggtcgg ccggcaccgg cacggccgcg agctcgcgcg ccagcgcggc cgcctgcgac
     3361 tcgagctcgg cgagcgatac gctcgtcgcc ttcgcctgcg cggcccaggc cggcggcccg
     3421 tagtaggcgt cgacgtagtt cgcgtcgtgt tgcccgagcg cgagcccgag cttcacgtag
     3481 cgctcggcgc aggcgtcgag cgacacgacc gcgggcgtcg cctggtcgct gaccgccggc
     3541 ttgcgcccag cgcaggcgac ggtcacgacc gcgagcgaga ggagcaggag acgcccggcg
     3601 agcagcatgc ccgcgagcgt cggtcagcct cgcgcgccgg tcaagcggcg gcgaccaggc
     3661 gatcactccg acgcgtgctc gcggaggagg cggcggcggt tgagcatgaa cagcccgagg
     3721 atgatcgccg cgccggtcat ccagcaggcg aggagcgtga gcagggtgga gggcgggacc
     3781 atgaagacag tgaggtacat gacgattctc cgtacgcgcg tcaggacctc tgccgcgctc
     3841 acggctatat gagcaagccg cgtgccagcg gccgatacg
//
LOCUS       DASWNJ010000628         2984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_223778, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000628 DASWNJ010000000
VERSION     DASWNJ010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223778"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1630
                     /locus_tag="VGB85_10540"
     CDS             170..1630
                     /locus_tag="VGB85_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134512.1"
                     /translation="MAKSEKPAAKAGGKTATKTASKATSKAPAKAGAKATAKTSTKAP
                     AAKATAKKVAKAEPKTSKAAAKAESKAEPKTESKAEPKASKAAAKVESKAEAKPAKAA
                     AKTTAKATRAEPQADKQDKPVAAARPEKAADKPAAAAPKAAASKAPAPKAPVRKPAKP
                     ADDGDDDDDGDFREAPTQASRNTIDMADVIRHEPEAPPAPRKKSVLEVAEEDEDFEED
                     PPLPTADDIHGLLDRLRKAAQYGGDALEDLPRLIEQLEQIDDPAVMPRLLGLLEDNDP
                     YGIYWNVLYVLEKFDDAYLKALLGSIEALHARAPQWAYTCIIRILNTRGDEDDCTAAF
                     ERLVNDGSGETRQLVLKVLRELSADPDTDHDQKQNIARTISAVGGNSSGGNSSGGTSG
                     GGNSGGGNSAGNTGSNPGSTGSTPGGTPGNNTGSTPSGAAGGTSSGAAGSNAGGNSAE
                     NAGGEPGESTGSAEGTVGDTAVGDTAVGDTTPSTAD"
     gene            1635..>2984
                     /locus_tag="VGB85_10545"
     CDS             1635..>2984
                     /locus_tag="VGB85_10545"
                     /inference="COORDINATES: protein motif:HMM:NF025038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0134513.1"
                     /translation="MAVPKPLQAERATTRVGAQDIPEIALAQASAESEINARIAPQGR
                     AVEALRRGLAERAQGFNVAVVGGRGTGRTFTAIALARAEAARRPAPRDLVLLTNPRWP
                     LEPVPAFLPPATGPGFVRAMTELHARLEAAIHEVFEGRVRSRLQLEVHREQSAAERKL
                     HDALGKIAAVHGMGLVANDDGFDFVPLEDQAGEGESVEETSAELESGEGITDDGGEDR
                     PTGISRQYLDAIEALRPHVEETQRQLALLEAESNAQLLHRQREALRRDIEASFVAIPT
                     RALPTPQLRQFTTDLHNHLREIYHLEEGHHLPLTAAPLPPGLVVPTLLITGATGEPAP
                     IVHAQNVTLSGLFGRVVAGSGEGRYPEPGTILAGDIHRANGGFLIIDAESLVKRERVY
                     EHLKACLLARSIQPYEDGEGPPLLRVQPVELDIKVVLVADPDLIIQLQELDPEFGRLF
                     "
BASE COUNT          529 a         1151 c          997 g          307 t
ORIGIN      
        1 cgtgacgcca tggccccgca gcatagcggt ggtgccgtgg ggcttgccac aggtgcggcc
       61 gatcgcgtag aaaaaccccg tggcagcatc gtcgacgcga tccaaaccag ccgccaagaa
      121 aaccgccagc aaggctgcga gcaagccggc ggccaagcgc agcagcgccg tggccaagtc
      181 ggagaagccc gcggccaagg ccggcggcaa gacggccacc aagacggcaa gcaaggcgac
      241 ctccaaggct ccggccaagg cgggggccaa ggcgaccgcc aagacctcta ccaaggcccc
      301 cgcggccaag gcgaccgcca agaaggtcgc caaggcggag cccaagacca gcaaggccgc
      361 cgccaaggcc gagtccaagg ccgagcccaa gaccgagtcc aaggccgagc ccaaggccag
      421 caaggccgcc gccaaggtcg agtccaaggc cgaggccaag cccgccaagg ccgccgccaa
      481 gacgaccgcc aaggcgaccc gcgccgagcc gcaggccgac aagcaggaca agccggtcgc
      541 cgctgcgcgg ccggagaagg ccgccgacaa gcccgccgcc gcggctccca aggccgccgc
      601 gagcaaggcg cccgcgccca aggctcccgt gcgcaagccg gccaagcccg cggacgacgg
      661 tgacgacgac gacgacggcg acttccgcga ggcgccgacc caggcgagcc gcaacacgat
      721 cgacatggcg gacgtgatcc gccacgagcc cgaggcgcct cccgcgccgc gcaagaagag
      781 cgtgctcgag gtcgccgagg aggacgagga cttcgaggaa gatccgccgc tcccgaccgc
      841 cgacgacatc cacggcctgc tcgaccgcct gcgcaaggcg gcccagtacg gcggcgacgc
      901 cctcgaggac ctgccgcgcc tgatcgagca gctcgagcag atcgacgacc ccgctgtcat
      961 gccgcgcctg ctcggcctgc tcgaggacaa cgacccctac gggatctact ggaacgtcct
     1021 ctacgtgctc gagaagttcg acgacgccta cctgaaggcg ctgctcggca gcatcgaggc
     1081 cctgcacgcc cgcgcgccgc agtgggccta cacctgcatc atccggatcc tcaacacccg
     1141 cggcgacgag gacgactgca ccgcggcgtt cgagcgcctc gtcaacgacg gcagcggcga
     1201 gacccgccag ctggtgctca aggtgctccg cgaactctcg gccgacccgg acaccgacca
     1261 cgaccagaag cagaacatcg cccgcacgat ctcggctgtc ggcggtaatt ccagcggcgg
     1321 taattccagc ggcggtacct ccggcggcgg taactccggt ggcggtaatt cggccgggaa
     1381 caccgggagc aatcccggga gcaccggcag cacccccggc ggcacccccg gtaacaacac
     1441 gggcagcacc ccgtccggag ctgccggtgg cacctccagc ggggctgccg gtagcaacgc
     1501 gggcggtaat tcggcggaga acgctggtgg cgagcccggt gagagcaccg gcagcgccga
     1561 gggcaccgtc ggcgacaccg ccgtcggcga caccgccgtc ggcgacacaa cgcccagcac
     1621 cgccgactga agtcatggct gtgcccaagc cgctccaggc ggagcgcgcc accaccaggg
     1681 tgggcgctca ggacatcccc gagatcgccc tcgctcaggc ctccgccgag agcgagatca
     1741 acgcgcggat cgccccgcag ggtcgcgccg tcgaggccct gcgacgcggc ctcgccgagc
     1801 gcgcccaggg cttcaacgtc gcggtcgtcg gcggtcgcgg caccggtcgc accttcacgg
     1861 cgatcgccct ggctcgcgcc gaggcggcgc gccggcccgc gccgcgggac ctcgtcctgc
     1921 tgacgaaccc gcgctggccg ctcgagccgg tgcccgcgtt cctgcccccg gcgacgggtc
     1981 cgggcttcgt gcgcgcgatg accgagctgc acgcccgcct cgaggccgcg atccacgagg
     2041 tcttcgaggg tcgcgtgcgc agccgactcc agctcgaggt ccaccgcgag cagagcgcgg
     2101 cggagcgcaa gctgcacgac gcgctcggca agatcgccgc cgtgcacggc atgggtctcg
     2161 tcgccaacga cgacggcttc gacttcgtcc cgctcgagga ccaggccggc gagggcgaga
     2221 gcgtcgagga gaccagcgcc gagctcgagt ccggcgaggg catcaccgat gacggcggcg
     2281 aggatcgccc gacgggcatc agccgccagt acctcgacgc gatcgaagca ctgcggccgc
     2341 acgtcgagga gacccagcgc cagctcgccc tgttggaggc cgagagcaac gcgcagctgc
     2401 tgcatcgcca gcgcgaggcg ctgcgccgcg acatcgaggc cagcttcgtg gcgatcccga
     2461 cgcgcgccct gccgaccccg cagctgcgcc agttcaccac cgacctccac aaccacctcc
     2521 gcgagatcta ccacctcgag gagggccacc acctgccgtt gacggccgcg ccgctgcccc
     2581 cgggcctcgt cgtgccgacc ctgctgatca caggagccac cggtgagccg gcgccgatcg
     2641 tgcacgcgca aaacgtcacg ctctcgggcc tgttcggccg cgtcgtcgcc ggatccggcg
     2701 agggccgcta ccccgagccc ggcacgatcc tcgcgggcga catccaccgc gccaacggcg
     2761 gctttttgat catcgacgcc gagtcgctgg tcaagcgcga gcgggtctac gagcacctca
     2821 aggcctgcct gctcgcgcgc tcgatccagc cctacgagga cggcgagggc ccgcccctgc
     2881 tgcgcgtgca gccggtcgag ctcgacatca aggtcgtgct cgtcgccgac cccgacctca
     2941 tcatccagct ccaggagctc gaccccgagt tcggccgcct cttc
//
LOCUS       DASWNJ010000629         3190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_470609, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000629 DASWNJ010000000
VERSION     DASWNJ010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470609"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1747
                     /locus_tag="VGB85_10550"
     CDS             <1..1747
                     /locus_tag="VGB85_10550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEY0134514.1"
                     /translation="MTVSASIPAAVMTVAVLRKRGVTLLEANMSQTIGSASTSLATGT
                     IFTIPALFLWGVAPPYWQIVALCFLGGLLGLAAMIPLRRLLIVDSHHELPYPEGTACA
                     EVLRATTTGTGGSAWIFRGIAVGALIKLAGALLFLMPTELRVALPVLPRGELAIEVAP
                     ALLAVGFILGYRQAAVVVAGAVVSALALIPLIAWIGAGLPGPLYPETRVLVAEMSASD
                     IWSRYIRYIGAGAVATAGILTVARNLPTMAGAFVAVLRGMRKGTGPHDERDPKDQDLP
                     GSFIGSAIAIVVLVVAFVPGVLAGDMGLAARLICAAGVAVFGLLFVTVAARIVGIVGV
                     SSQPTSGITLVTLLGTASLFAAFGWIDTGARAAVLTVGTIVAVAASKAGDISQDLKTG
                     FLVGATPARQQLGQLIGAACACWAVAATVMLLSSYGFGSKELPAPQATLMKTIIEGVL
                     SGELPWGLVMTGGGVTIAAMLCGVSGLAFAIGVYLPLASMAPIFVGGCVRAFSERGRP
                     KVHGPEGEARAGILAASGVVAGEGLAGVLVAGLVAAGVIAKSGKPVLDGASGEVVSLL
                     AIAGVCLFLGRAARA"
     gene            1780..2994
                     /locus_tag="VGB85_10555"
     CDS             1780..2994
                     /locus_tag="VGB85_10555"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEY0134515.1"
                     /translation="MRRLVILGCCLVACGSEPATTDVGTSGTTSTGTTSETSGEVDTT
                     AVPTSMPEETGTTGEVVTTGDAGTTGTTGTTGTSTTGDTSTDEATTGEELPPVKPRGL
                     VNYVTGEPADAVVRTSGPALIVMGGGTDVDAAFTWQLQYMQGGDVVVLRTSGADGYND
                     YLFTDIGGVDSVETLMVTSEALAQDPYVAWTIAHAEAVFMAGGDQATYLNNWKDTAVE
                     DALHEVWARGGVVGGTSAGTAVLSEFVYAAYNDSVIEDEALLDPYNQYMTFERDFLAL
                     APLQGVITDTHFTQRRRLCRLIGFVARIVADGWGEPVLGIGVDEQTALVVGPDGVGSV
                     LGAGGVHLLHSAGTPQQCAPGLTLEYADLDHYTLKAGDSVQLPAGVPEGVQAEKLSAS
                     MGALDPAYNCVK"
BASE COUNT          407 a         1003 c         1277 g          503 t
ORIGIN      
        1 catgacggtg tcggcttcga tcccggcggc ggtgatgacg gtcgcggtgc tgcgcaagcg
       61 cggcgtcacg ttgctcgagg ccaacatgtc gcagacgatc ggctcggcct cgacctcgct
      121 ggcgaccggc acgatcttca ccattccggc tctcttcctc tggggggtcg cgccgccgta
      181 ctggcaaatt gtcgcgctgt gcttcctcgg cgggctgctg ggcctggcgg cgatgatccc
      241 cctgcggcgc ctgctgatcg tcgacagtca tcacgagctg ccctacccgg agggcacggc
      301 ctgcgcggag gtgctgcgcg cgacgacgac cggcacgggc ggcagcgcgt ggatcttccg
      361 tgggatcgcc gtcggggcgt taattaaact cgcgggggcg ctgctgttcc tgatgccgac
      421 cgagctgcgc gtggcgttgc cggtgctgcc gcgcggcgag ctggcgatcg aggtcgcacc
      481 ggcgctgctg gccgtcggct tcatcctcgg ctatcgccag gcggcggtgg tcgtcgccgg
      541 cgcggtggtc tcggcgctgg ccttgatccc gctgatcgcg tggatcggcg ccgggctgcc
      601 cgggccgctg tacccggaga ccagggtgct ggtcgcggag atgagcgcga gcgacatctg
      661 gtcgcgctac atccgttaca tcggcgccgg tgcggtcgcc accgcgggga tcctgacggt
      721 cgcgcgcaac ctgccgacga tggccggcgc gttcgtggct gtccttcggg gcatgcgcaa
      781 ggggacaggt ccgcacgacg agcgcgaccc gaaggaccag gatctgccgg gcagcttcat
      841 cggctcggcg atcgcgatcg tggtgctggt ggtcgcgttc gtgcccgggg tgctcgcggg
      901 cgacatgggg ctcgcggcgc ggctgatctg cgccgccggg gtggcggtgt tcggcctgct
      961 gttcgtgacg gtcgcggcgc ggatcgtcgg catcgtcggg gtgtcgtcgc agccgacctc
     1021 ggggatcacg ctggtgacgc tgctcggcac ggcgtcgctg ttcgccgcgt tcgggtggat
     1081 cgacaccgga gcgcgcgcgg cggtgctgac ggtcggcacg atcgtcgcgg tcgcggcctc
     1141 gaaggcgggc gacatctcgc aggacctcaa gaccggcttc ctcgtcggcg cgacgccggc
     1201 ccgccagcag ctcgggcagc tgatcggggc cgcgtgcgcg tgctgggcgg tcgcggcgac
     1261 ggtgatgctg ctgtcgagct acggcttcgg cagcaaggag ctgccggcgc cgcaggcgac
     1321 cctgatgaag acaattattg aaggcgtgct cagcggggag ctgccgtggg gcctggtgat
     1381 gaccggcggc ggggtgacga tcgcggcgat gctgtgcggg gtctcggggc tggcgtttgc
     1441 gatcggggtg tacctgccgc tcgcctcgat ggcgccgatc ttcgtcggcg gctgcgtgcg
     1501 cgcgttctcg gagcgcggcc ggccgaaggt gcacgggccc gagggggagg cgcgggcggg
     1561 gatcctcgcg gcgtcgggcg tggtcgcggg cgaggggctc gcgggtgtat tggtcgccgg
     1621 tctggtcgcg gcgggcgtga tcgccaagtc gggcaagccg gtgctcgacg gcgcgtccgg
     1681 cgaggtcgtg tcgctgctgg cgatcgcggg ggtgtgtctg ttcctcgggc gagccgcgcg
     1741 cgcttgacgg gcccaggaga ccgggagata ccggcgagca tgcgacggct ggtgatcctc
     1801 gggtgctgtc tggtggcgtg tgggtcggag ccggccacga ccgacgtggg cacgtccggg
     1861 accacgagca cgggaacaac gagcgagacg agcggcgagg tcgacaccac ggctgtcccg
     1921 acgagcatgc ccgaagagac aggtacgacg ggcgaggtcg tgacgacggg agacgccggc
     1981 acgacgggca cgaccggtac gaccggtacg agcacgacgg gcgacacgag caccgacgag
     2041 gcgacgaccg gcgaggagct accgccggtc aagccgcgcg ggctggtcaa ctacgtcacc
     2101 ggggagccag cggacgcggt ggtgcgcacc agcggtccgg cgctgatcgt gatgggtggg
     2161 ggcacggacg tcgatgcggc gttcacctgg cagttgcagt acatgcaggg cggcgacgtc
     2221 gtggtgctgc ggacctccgg cgccgatggt tacaacgact atttgttcac ggacatcggc
     2281 ggggtcgact cggtcgagac gttgatggtc accagcgagg cgctcgcgca ggacccctac
     2341 gtcgcgtgga cgatcgcgca cgccgaggcg gtgttcatgg ccggcggcga tcaggcgacc
     2401 tatctcaata actggaagga cacggcggtc gaggacgcgc tgcacgaggt gtgggcgcgc
     2461 ggcggggtgg tcgggggcac gagcgcgggc acggcggtgc tctcggagtt cgtgtacgcg
     2521 gcatacaacg actcggtgat cgaggacgag gcgctgctcg acccgtacaa ccagtacatg
     2581 accttcgagc gcgacttcct ggcgctcgcg cctctgcaag gggtcatcac ggacacgcac
     2641 ttcacgcagc ggcggcggct gtgccggttg atcggcttcg tcgcgcggat cgtcgccgac
     2701 ggctggggtg agccggtgct cgggatcggg gtcgatgaac agacggccct ggtggtcggg
     2761 cccgacggcg tcggcagcgt gctcggggcc ggcggcgtgc acctgctgca cagcgccggc
     2821 acgccgcagc agtgcgcgcc ggggctgacg ctcgagtacg cggacctcga ccactacacc
     2881 ctgaaggccg gcgacagcgt ccagctgccc gcgggggtcc ccgaaggggt ccaggccgag
     2941 aagctgtcgg cgagcatggg ggcgctcgat ccggcctata actgtgtaaa gtgaccgcga
     3001 cgccgttgca gacgtcggcg cgtcgggtac ggtgtcgcca tgatccgtgc gtcgctcgtc
     3061 gtcgtggtcc ttgcgagcgg ctgcgccgct tactcgccgc cggtgcgcag cctcggttac
     3121 ggcgcgccgg ggcggctgcg gcagggcgag ctcgaggtcg gtggtggctt cgcggcggcg
     3181 ccggtgcccg
//
LOCUS       DASWNJ010000630         1986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_152850, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000630 DASWNJ010000000
VERSION     DASWNJ010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152850"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /locus_tag="VGB85_10560"
     CDS             complement(<1..1225)
                     /locus_tag="VGB85_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134516.1"
                     /translation="MSYRPRPALWLCLALPACTPETGTETGDATTTTATTSADASSGT
                     PTGTPTTGATSDTGDSSESGTAEPLMPLPPLADPVPLTDTQLRVPTHVRDEEHLIDPR
                     IPADVTQALADGYGEITLEPGEPVLPRTMDDTPAPAPGAAPKLLVRFVHLADTQLSDD
                     ESPARLASYDEAADGAFRPQEGHLCRMLNAAVRTINRIHVDSPLDFVLLGGDNTDNAQ
                     SNELEWFMSILDGAAQVECDSAVDNDIVPGPDNDPKDPFGPVALDVPWRWVTGNHDLL
                     RQGTWPTPAYVGEPIGVTAMGGTRDYSLPGSPIVTGTVVADPARAFVTEGEQLARVAA
                     AADGHGITPEALALGRAYYTFDIEGTPLRIFVMSTAAATGSSKGLIRQADIDTIIEPT
                     LQAAAADDKFVILTSH"
     gene            complement(1259..>1986)
                     /locus_tag="VGB85_10565"
     CDS             complement(1259..>1986)
                     /locus_tag="VGB85_10565"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEY0134517.1"
                     /translation="RAAELTTVSVRLACEAREQYARTHPEGAYVRIAGAVSPLEDCFR
                     PADSPGHDAGPEHREIAEHLLRAGADLLLIETMGRIDEARAAVAAAQGLGRPVWLAVV
                     ARADGRMLAGESLDDLVAALADLELHALLLNCTELRHLDVALPALCAAAAVRPALWLG
                     AYPHTGHADPERGWQTHATTAEEFADTLASWSVRLPQLRVLGGCCGSTPAWISALASR
                     LQPDAASRTAAFAALADAARPGT"
BASE COUNT          251 a          660 c          798 g          277 t
ORIGIN      
        1 ggtgcgaggt gaggatcacg aacttgtcgt cggcggccgc ggcctgcagc gtcggctcga
       61 tgatcgtgtc gatgtcggcc tggcggatca ggcccttgga ggagccggtc gccgccgcgg
      121 tgctcatcac gaagatccgc agcggcgtgc cctcgatgtc gaaggtgtag taggcgcggc
      181 cgagcgcgag cgcctccggc gtgatgccgt gaccgtccgc ggccgcggcg acgcgcgcca
      241 gctgctcgcc ctcggtgacg aaggcgcgcg cgggatcggc gaccacggtg ccggtgacga
      301 tcggcgagcc gggcaggctg tagtcgcggg tgccgcccat cgcggtcacg ccgatcggct
      361 cgccgacgta ggccggggtc ggccaggtgc cctggcgcag caggtcatgg ttgccggtga
      421 cccagcgcca gggaacatcg agcgcgaccg ggccgaacgg gtccttgggg tcgttgtcgg
      481 ggccaggcac gatgtcgttg tcgacggccg agtcacactc gacctgcgcc gcgccgtcga
      541 ggatcgacat gaaccactcg agctcgttgc tctgcgcgtt gtcggtgttg tcgccgccga
      601 gcaggacgaa gtccagcggg ctgtcgacgt ggatgcggtt gatcgtgcgc accgcggcgt
      661 tgagcatgcg acacaggtgg ccctcctgcg ggcgaaacgc gccgtcggcg gcctcgtcgt
      721 agctggccag gcgcgccggg gactcgtcgt cggacagctg cgtgtccgcc agatggacga
      781 agcgcaccag cagcttcggc gcggcgccgg gggccggggc gggcgtgtcg tccatggtcc
      841 gcgggagcac cggctcgccg ggctcgagcg tgatctcgcc gtaaccgtcg gcgagcgcct
      901 gggtgacgtc ggcggggatc cgcgggtcga tcaggtgctc ctcgtcgcgc acgtgcgtcg
      961 gcacgcgcag ctgcgtgtcg gtcaacggca ccgggtccgc gagcggaggc aagggcatca
     1021 gcggctccgc agtgccgctc tcgctgctgt cgccggtgtc gctggtcgcc ccggtggtcg
     1081 gggtgccagt cggggtcccg ctgctggcgt cggcgctcgt ggtcgccgtc gtggtggtcg
     1141 cgtcgcccgt ctcggtgccg gtctcggggg tgcaggcggg cagcgcgagg cagagccaca
     1201 gggcggggcg agggcggtat gacatcacgg caggctatca gggatgtccg cgccccggtc
     1261 atgttcctgg ccgcgccgca tcggcgagcg cggcgaaggc cgcggtccgc gacgcggcgt
     1321 cgggctgcag gcgcgaggcg agcgcggaga tccacgccgg ggtcgatcca caacagccgc
     1381 cgagcacgcg cagctgcggc aggcggaccg accacgaggc gagcgtatcg gcgaattcct
     1441 ccgcggtggt cgcgtgggtc tgccagccgc gctcggggtc cgcgtggccg gtgtgcgggt
     1501 aagcgccgag ccacagggca gggcgcaccg cggccgcggc acacagggcc ggcagggcga
     1561 cgtcgaggtg gcgcagctcg gtgcagttga gcagcagcgc gtgcagctcg aggtcggcga
     1621 gcgcggcgac gaggtcgtcg agagactcac cggcgagcat gcggccgtcg gcgcgggcga
     1681 ccacggcgag ccacaccggg cgcccgaggc cctgcgcggc ggcgacggcg gcgcgggcct
     1741 cgtcgatgcg acccatggtc tcgatcaaca gcaggtcggc cccggccctg agtaaatgct
     1801 cggcgatctc gcggtgctcc gggccggcgt cgtgaccggg cgagtccgcg gggcgaaagc
     1861 agtcctcgag cgggctgacc gctcccgcga tccgcacata tgcgccctcg ggatgggtgc
     1921 gtgcatattg ctcgcgggcc tcgcacgcga ggcgcaccga cactgtcgtc agttcggccg
     1981 cgcgac
//
LOCUS       DASWNJ010000631         6156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_542090, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000631 DASWNJ010000000
VERSION     DASWNJ010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6156
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542090"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VGB85_10570"
     CDS             complement(<1..666)
                     /locus_tag="VGB85_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134518.1"
                     /translation="MLPAIRRLAAPVALASLLPACPAPATGEDGSSGTTAEPTSTTEV
                     DPTTPATGSTSDAPTTTGDPSTGAPPDTTDTTDTTGAPVEPTWLQVFDAPDGQHPGGL
                     GLAADGSLWVAGDFYGSIDLGLGALAGEGTGLYLARFTATGEPTHAQAIFPADGAPTL
                     TQIAGLGVGSDGSVVVSGWLEGTYQLGGDTLVADEVDVHVAKWDVDGAPLWGQRFGQA
                     DWQV"
     gene            745..1494
                     /locus_tag="VGB85_10575"
     CDS             745..1494
                     /locus_tag="VGB85_10575"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY0134519.1"
                     /translation="MASEASYELYYWPGLPGRGEFIRLVLEDAGATYVDVARLPEDQG
                     GGAGAITRLLRGEHTGQLPLAPPILRHGELTIAQVANICMYLGPRHGLAPADEPGRLA
                     CNQLQLTVADLVGEVHDTHHPIAVGRYYEQQKVAARERAAFFLDQRLPRFLGHFEKVL
                     GRNREGGGRFLVGTSLSYADLSVFHVLEGLAYAFPRAFARNEATIPGLLGLRERVRER
                     PGIAAYLASDRRLPFNEKGIFRHYPELDLPG"
     gene            1502..2059
                     /locus_tag="VGB85_10580"
     CDS             1502..2059
                     /locus_tag="VGB85_10580"
                     /inference="COORDINATES: protein motif:HMM:NF017747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HEY0134520.1"
                     /translation="MPTHLRPEDYRRMRWKNGGGWTSEVAVRAPSHSGQGEDFAWRIS
                     IAEIETDGDFSPFPGVARGILVLDGAGMTLEVAGAPVELRPDGPALAFAGEDAVYCRL
                     SGGPTRDFNVMTRRGRYVHTLERHALAGSLGLGGECLVYVHSGAAQAGEYMLGTGDSL
                     RVEAGDEPVTLRGTAALVIARLRAV"
     gene            2148..3071
                     /locus_tag="VGB85_10585"
     CDS             2148..3071
                     /locus_tag="VGB85_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134521.1"
                     /translation="MRPLLLLTIVALTGCHSPEYQKYIDQQEQWASTGSESTGTAPPD
                     PGHDTSEAEASAGAEASAEASTDESTEGTGSGGGDETTGDGTSNEPGETTASATTTEP
                     DPVCGDGVVDPKSEECDDGLADPEGACSLFCQRSRLIFVTSIRINGKMSGLQGADAYC
                     KSQALKAQMADPASPIVDANNFKALLPSSTETIFGRHFHGKGPYRLVNGLKVSDSFIE
                     LFTEPLKNPINVDEFSDTQITSVWTASLANGSAYPGVDFCGNWTGTKGSASKGRSEYT
                     DAWWIEVSTEVAGQPTTDCANDWALYCVEQQ"
     gene            3074..4249
                     /locus_tag="VGB85_10590"
     CDS             3074..4249
                     /locus_tag="VGB85_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134522.1"
                     /translation="MNRIITGTFTLFLAGCPAHPGSPEAADTGTGSSSSGEDGPPDDT
                     PTGSPTNDPTEPSTAGESPTTGDSTSTGAIGACGDGFVDAGEGCDEGAANGPYAACTD
                     GCQVNVCGDGKVLVGVEGCDEGPANVDDGYCRSNCQLGVCGDGFVFAGVEECDAAVLG
                     LAYGQCDEQCTINRCGDGELDVGFEECDEGELNGSGGGGEMGLAGCDIDCGFAGRRIF
                     LSSQTFTGDMGTRAGADLACETMAYTAGLRHSERYRALLADASGGPNDFVQADPQDDR
                     PFILPTGLVLAASYPDLLASGPGEGVTTTEQGEVLLETRVWTNVNPAGDAYLEEPAST
                     CASWSSADGLKAARVGYNAVAPGDAAALAEWKTKKQWLSFSLEACSESFRIYCIEAS"
     gene            4376..5950
                     /locus_tag="VGB85_10595"
     CDS             4376..5950
                     /locus_tag="VGB85_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY0134523.1"
                     /translation="MRDDDEPRESGAGERLRLDHRTEALIRAALPGMAGRLSFGDELA
                     RGGMGAVRVAFDAALQRRMAAKVMHEASYERLMLVHGFLREAQVTGQLDHPNIVPIYE
                     LGRDERGGLYFTMKLIEGQSLRALIGRRPPANYEALYNRLQIFIKVCDALAFAHSRGV
                     IHCDIKSANVMVGEYGEVLLMDWGGARLLEPPPGRDTSGWVHDSLPPLSKYDTDGLVL
                     GSPSYMSPEQAGGVGVPLDERSDVFSMGALLYEMMTGRPPYASSSAWESLALARAAEI
                     VPPDQLPGMPVHVYLGELLRIVMKALERRPEDRYASVDGFKQDLFRLFRGASNFETVQ
                     FAPGTYVIREGEHGDAAYMVVSGKLEVSKQVDGTRRVLRELGPGEVFGETAIFAASRR
                     TASVRVVEQASLIAVNSQVMEQELLSMKPWMSAFIRTLASRFGGLDGAASIVAPPSQP
                     EQPGEMTVVADVMSDLEDEVESEAIEEIDPELDDDRTGPIEKTARDPPAPQVEDEDDA
                     LTHALELTSQVANKPA"
BASE COUNT          918 a         1971 c         2381 g          886 t
ORIGIN      
        1 gacctgccag tcggcctggc cgaagcgctg gccccacagc ggcgcgccgt cgacatccca
       61 cttggcgacg tgcacgtcga cctcgtcggc gacgagcgtg tcgccgccga gctggtaggt
      121 gccctcgagc cagccagaga cgacgacgga gccgtcgctg ccgacgccga gacctgcgat
      181 ctgcgtcagc gtcggcgcgc cgtcggcggg gaagatcgcc tgggcgtgcg tgggctcgcc
      241 ggtggcggtg aagcgcgcga ggtacaggcc ggtgccctcg ccggcgagcg cgccgaggcc
      301 gaggtcgatg ctgccgtaga agtcgccggc gacccacagc gagccgtcgg cggcgaggcc
      361 gaggccgccg gggtgctggc cgtcgggcgc gtcgaagacc tgtaaccagg tcggctcgac
      421 cggggcgccg gtggtgtcgg tggtgtcggt ggtgtcgggc ggcgcgccgg tgctggggtc
      481 gccggtcgtg gtcggggcgt cgctcgtcga gccggtggcg ggcgtggtcg ggtcgacctc
      541 ggtggtgctg gtcggctcgg cggtggtgcc ggagctgccg tcctcgccgg tcgccggggc
      601 cgggcaggcg ggcaggagcg aggcgagggc cacgggggcg gcgaggcggc ggatcgcggg
      661 gagcatggtc ggcttgtaac acgcggacgc ggcgggtcgc ggcgcttgcg atcgcggcgg
      721 gcccggccta tgacggtgct cgccatggca agcgaagcgt cctacgagct gtattactgg
      781 ccgggtctgc cgggtcgcgg cgagttcatt cgcctcgtgc tcgaggacgc gggggcgacg
      841 tacgtcgatg tcgctcgcct gccggaggat cagggcggcg gggccggtgc gatcacgcgg
      901 ctgctgcgcg gcgaacatac cgggcaattg cccctggcgc cgccgatcct gcgccacggc
      961 gagctgacga tcgcgcaggt cgccaacatt tgtatgtacc tcgggccgcg ccacgggctc
     1021 gcgcccgcgg acgagcccgg ccgtctggcg tgtaatcaat tgcaattgac ggtcgcggac
     1081 ctcgtcggcg aggtccacga tacgcaccac ccgatcgcgg tcggccgcta ttacgagcag
     1141 caaaaggtgg cggcccgcga gcgcgcggcg ttcttcctcg accagcgcct gccgcggttc
     1201 ctcggccatt ttgaaaaggt gctcgggcgc aaccgcgagg gggggggacg gttcctggtc
     1261 ggcacgagcc tgagctacgc ggatctgtcg gtgtttcatg tgctcgaggg gctcgcgtac
     1321 gcgttcccga gggcgttcgc gcgcaacgag gcgacgatcc cggggctgct cgggctgcgc
     1381 gagcgggtcc gcgagcggcc gggcatcgcc gcgtacctgg cgtcggaccg ccgcctgccg
     1441 ttcaacgaga agggcatctt tcgccactac cccgagctcg acctgccggg ctgatcgacc
     1501 gatgccgacg catctacgac cggaggatta ccggcgcatg cgctggaaga acggcggcgg
     1561 ctggacctcg gaggtcgccg tgcgcgcccc ctcgcattcg ggccaggggg aggatttcgc
     1621 gtggcggatc tcgatcgcgg agatcgagac cgacggcgat ttctcgccgt ttcccggggt
     1681 ggctcgcggc atcctcgtgc tcgacggggc ggggatgacc ctcgaggtcg cgggggcgcc
     1741 ggtcgagctg cggccggacg ggccggcgct cgcgttcgcg ggcgaggacg cggtatattg
     1801 tcgattgagc ggcgggccga cgcgcgactt caatgtgatg acccggcgcg ggcgatatgt
     1861 gcatactctg gagcgccacg cgctcgcggg gtcgctgggg ctcggcggcg aatgtctggt
     1921 gtacgtgcat tcgggggcgg cgcaagccgg cgaatacatg ctggggacag gggatagcct
     1981 gcgggtcgag gccggggacg agccggtgac gctgcgcggg acggcggcgc tggtgatcgc
     2041 gcggctgcgc gcggtctgag gggtgcaaat ggcaagtgcg gacagcgtta ttccccggcg
     2101 agcgcgatgg gcttgacgcg cgatttcata cgtgcgacgc tcgcgcggtg cggcctctgc
     2161 tcttgctgac tatcgtggcg ctgacgggct gccactcgcc cgagtaccag aaatatatcg
     2221 accagcagga gcagtgggcg agtacgggct ccgagtcgac cggcacggcc ccacccgacc
     2281 cgggtcatga caccagcgag gccgaggcga gcgcgggcgc ggaggcgagc gcggaggcca
     2341 gcaccgatga gagcaccgaa gggaccggga gcggcggcgg tgacgagacc accggcgacg
     2401 gcacgtcgaa cgagcccggg gagacgaccg cgagcgccac gacgacggag cccgacccgg
     2461 tgtgcggcga cggtgtggtc gacccgaagt ccgaggagtg cgacgacggg ctcgccgacc
     2521 cggagggcgc gtgctcgctg ttttgccagc gttcgcgcct gatcttcgtc accagcatcc
     2581 gcatcaacgg caagatgagc gggctccagg gggcggacgc gtattgtaaa tcgcaggcgc
     2641 tcaaggccca gatggccgac cccgcctcgc cgatcgtcga cgccaacaac ttcaaggcgc
     2701 tgctgccgag ctcgacggag acgatcttcg gccgccattt ccacggcaag gggccatacc
     2761 ggctcgtcaa cgggctcaag gtcagcgaca gcttcatcga gctgttcacc gagccgctca
     2821 agaacccgat caacgtcgac gagttcagcg acacgcagat caccagcgtg tggaccgcct
     2881 cgctcgccaa cggctcggcc taccccggcg tggacttctg tggcaattgg accggcacca
     2941 agggctccgc gagcaagggc aggagcgaat acaccgacgc gtggtggatc gaggtctcga
     3001 ccgaggtcgc agggcagccg acgaccgact gcgccaatga ctgggcgctt tattgcgtgg
     3061 agcagcagta gtcatgaacc gtatcatcac aggcacgttc accttgttcc tcgcgggctg
     3121 ccccgcgcat cccggttcgc ccgaggccgc ggacaccggg accggctcgt cgtcgagtgg
     3181 cgaagacggc ccaccggacg acaccccgac cggcagcccg acgaacgatc cgacggagcc
     3241 gagcacggcc ggcgagagcc cgacgaccgg ggacagcacg agcaccggcg cgatcggtgc
     3301 gtgtggtgat ggtttcgtcg acgccggcga gggctgcgac gagggcgcgg cgaacggccc
     3361 ttacgcggcg tgcaccgatg gttgccaggt caacgtgtgc ggcgacggaa aggtgctggt
     3421 cggcgtcgag ggctgcgacg agggacccgc caacgtcgac gacggctact gtcgatcgaa
     3481 ctgtcagctc ggcgtctgtg gcgacggctt cgtgttcgcc ggggtcgagg agtgcgatgc
     3541 ggcggtcctc ggactcgcgt atggccagtg tgacgagcag tgcacgatca atcgttgcgg
     3601 cgacggcgag ctcgacgtcg gcttcgagga gtgcgacgag ggtgagctca acggctccgg
     3661 cggcggcggt gagatgggtc tggccggctg cgacatcgac tgcgggttcg cggggcggcg
     3721 gatcttcctg tcgtcgcaga cctttaccgg cgacatgggc acccgcgcag gcgcggacct
     3781 ggcctgcgag accatggcct acacagccgg tcttcggcac agcgagcgct acagggccct
     3841 gctggccgat gccagcggcg ggcccaacga cttcgtgcag gccgacccgc aggacgaccg
     3901 gcccttcatc ctgcccaccg gcctcgtgct cgccgccagc tatcccgacc tgctcgcgtc
     3961 ggggcccggc gagggcgtca cgaccaccga gcagggcgag gtgctgctcg agacgagggt
     4021 ctggaccaac gtcaacccgg cgggcgacgc ttacctcgag gagcccgcct cgacctgcgc
     4081 gagctggagc tcggcggacg ggctcaaggc ggcgcgcgtc ggctataacg cggtcgcgcc
     4141 gggcgatgcg gccgcgctgg ccgagtggaa aaccaagaaa cagtggctca gtttttcgct
     4201 ggaagcctgc agcgagtcgt ttcgcatcta ctgcatcgag gcctcgtagc gcgtcgccga
     4261 cgggatgcgc aacatcgggc ggacggggtc cgacaccgcg cgctggtggc gcgtgacgcg
     4321 gtcgggttcg tgcgcgggag cggtgtacga tgggcctcct cgcggaggag tgtggatgcg
     4381 cgacgatgat gaacctcgag agtccggtgc gggtgagcgg ctcaggctcg accaccgaac
     4441 ggaggcgctg atccgggccg cgctgccggg gatggccggg cgcctgagct tcggtgacga
     4501 gctggcgcgc ggcgggatgg gggccgtgcg ggtcgccttc gacgcggctc tgcagcgccg
     4561 catggcggcg aaggtgatgc acgaggcgag ctacgagcgc ctgatgctcg tgcacgggtt
     4621 cctgcgcgag gcgcaggtga cgggtcagct cgaccacccg aacatcgtgc cgatctacga
     4681 gctcggccgc gacgagcgcg gcgggctcta cttcacgatg aagctgatcg aggggcagag
     4741 cctgcgcgcg ctgatcggtc ggcgtccgcc ggccaactac gaggcgctgt acaaccggct
     4801 gcagatcttc atcaaggtgt gcgacgcgct ggccttcgcg cacagccgcg gggtgatcca
     4861 ctgcgacatc aagtcggcca acgtgatggt gggcgagtac ggcgaggtcc tgctgatgga
     4921 ctggggcggc gcgcgcctgc tcgagccgcc gccggggcgg gacacctcgg gctgggtgca
     4981 cgactcgctg ccgccgctgt cgaagtacga caccgatggc ctggtgctcg gctcgccgtc
     5041 gtacatgtcg ccggagcagg cgggcggggt cggggtgccg ctcgacgagc gcagcgacgt
     5101 gttctcgatg ggcgcgctgc tctacgagat gatgaccggg cgcccgccgt acgcgagcag
     5161 cagcgcctgg gagtcgctgg cgctggcgcg cgcggccgag atcgtgccgc cggatcagct
     5221 gccggggatg ccggtgcacg tgtatctcgg cgagctgctg cgcatcgtca tgaaggcgct
     5281 cgagcggcgc ccggaggacc gctatgccag cgtcgacggg ttcaagcagg acctgttccg
     5341 gctgttccgc ggcgcgagca acttcgagac ggtccagttc gcgccgggca cgtatgtcat
     5401 tcgggagggc gagcacggcg acgccgcgta catggtggtc tccggcaagc tcgaggtgtc
     5461 gaagcaggtc gacggcacgc gccgcgtgct gcgcgagctc gggcccggcg aggtgttcgg
     5521 cgagaccgcg atcttcgcgg cctcgcggcg caccgcgagc gtgcgcgtgg tcgagcaggc
     5581 gtccctgatc gcggtcaaca gccaggtgat ggagcaggag ctgctgtcga tgaagccgtg
     5641 gatgagcgcg ttcatccgca cgctggcgag ccgcttcggc gggctcgacg gcgccgcttc
     5701 gatcgtcgcg ccgccgtcgc agccggagca gcccggggag atgacggtgg tcgccgacgt
     5761 gatgagcgac ctcgaggatg aggtcgagtc cgaggcgatc gaggagatcg atccggagct
     5821 cgacgacgat cgaaccgggc cgatcgagaa aacggcccgc gatccgccgg ccccgcaggt
     5881 cgaggatgag gacgacgcgc tgacccacgc gctcgagctg acctcgcagg ttgcgaacaa
     5941 gcccgcctag acgggcgagc gagccctgcg aggggagaag ctgtcccgtg ggacatcccg
     6001 ggagcgtcac gcggcgctga gccagtgggc gagggtgctg cgggggatcg ccagctgggc
     6061 ggcggccttg cggatcgacc cggcctcgtc gacgacctga tgcaggaggg cgcggtgcat
     6121 ctgcgcccag gtgccgcgcg gcacggcgag cgccgg
//
LOCUS       DASWNJ010000632         2216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_47135, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000632 DASWNJ010000000
VERSION     DASWNJ010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47135"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1464
                     /locus_tag="VGB85_10600"
     CDS             <1..1464
                     /locus_tag="VGB85_10600"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0134524.1"
                     /translation="ESGMGAVFRARHLKLGREVAIKRLNPEYSACTLLVERFENEAKA
                     VIQIEDPHICDVNDVGFDPQIGHYIVMEWLAGGSLRGLLRMLGRVPYQSAVQLIRHLL
                     LGLDATHRAGIVHRDIKPENAFVVTRQPWTASLDLSAVDVKLLDFGIARMAGTPGFMT
                     MMGPMTVMYAAPEQAIQPTAVTSTADLWAVGVVLYELIVGKLPFADTDPGSAPWQAVA
                     SRGGLVSSRPSPMHKFVPEVPPRLSEVVDRCLLVDASRRYLSAQELSAALAPFETVDP
                     IAPTELGGALQMVAARSSLRAFGSYAGGIILVVGASLALVWVLDPSRVADPEVVDVPQ
                     PTQRVDPPVAGPPGPAPELAKPPVIEAPPVVEASVAPVTPTSAPRPDKQAPKPRPRPV
                     KDPPAKAGPPLFDVAVAKSKLEQFMRDHAPACWAANPDAPNLQVTVQVSPVGEVTIKQ
                     AANSAIRECLFERASKLTLGPSVDGGEFNYRFVRPGG"
     gene            complement(1467..>2216)
                     /locus_tag="VGB85_10605"
     CDS             complement(1467..>2216)
                     /locus_tag="VGB85_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134525.1"
                     /translation="AEAVGKFEEAYRCMPRDRKHDARWDRIAAYLDDVSQVPTASPEE
                     LHTQHCRTRALIEDYLASIPTDPRPKSAQYAEKELQKIEQTLATRSCPEPAAPARPER
                     PKAIELLDGPGAPRTDTPRSDPAATTPDRAVVHAPAAANPARPLWIAAGVTGGLTLAS
                     AVLLGAGVSRGTRAEQAADQHHDAGTLLQLRDEVYARGASGNRLAIAGASLTAVLLVT
                     TVALLVVGSRRSPPQRPLGLRVGAAGISVRF"
BASE COUNT          301 a          753 c          832 g          330 t
ORIGIN      
        1 gagagcggga tgggggcggt gttccgcgcg cgtcacctca agctcggccg cgaggtcgcg
       61 atcaagcggc tgaatccgga gtacagcgcg tgcacgctgc tggtcgagcg gttcgagaac
      121 gaggcgaagg cggtgatcca gatcgaggac ccgcatatct gcgatgtcaa cgatgtcggg
      181 ttcgacccgc agatcggcca ttacatcgtg atggagtggc tggcgggcgg ctcgctgcgc
      241 gggctgctgc ggatgctcgg gcgggtgccg taccagtcgg cggtgcagct gatccgccat
      301 ctgctcctcg gcctcgacgc gacgcaccgg gccggcatcg tgcaccgcga catcaagccc
      361 gagaacgcat tcgtggtgac ccgccagccg tggacggcga gcctcgatct gtcggcggtc
      421 gacgtcaagc tcctcgactt cggtatcgcg cggatggccg ggaccccggg cttcatgacg
      481 atgatgggcc cgatgacggt gatgtacgcc gccccggagc aggcgatcca gccgacggcg
      541 gtgacctcga ccgccgacct gtgggcggtc ggcgtcgtcc tgtatgaatt gatcgtcggc
      601 aagctgccgt tcgccgacac cgacccgggc agcgcgccgt ggcaggcggt cgcgtcgcgc
      661 ggcggcctgg tcagcagccg gccgtcgccg atgcacaagt tcgtcccgga agttccgccc
      721 aggctgtccg aggtcgtcga ccgctgcctg ctggtcgacg cgagccggcg ctacctgtcc
      781 gcgcaggagc tgtcggcggc gctggcaccg ttcgagacgg tcgatccgat cgcgccgacc
      841 gagctgggcg gggcacttca aatggttgcg gcacgctcgt cgctgcgggc gttcgggagc
      901 tacgctggcg gcatcatcct ggtggtcggc gcgtcgctgg cgctggtgtg ggtgctcgat
      961 ccgtcgcggg tcgccgatcc cgaggttgtc gacgtcccgc agccgacgca gcgcgtcgat
     1021 ccgccggtcg cgggaccgcc cgggcccgcg ccggagctcg cgaaaccgcc ggtgatcgag
     1081 gcgccgccgg tggtcgaggc gtcggtcgca ccggtcacac cgacctcggc gccgcgtccg
     1141 gacaaacagg cgccgaagcc ccgaccgagg ccggtgaaag atcctccggc gaaggccggc
     1201 ccgccgctgt tcgacgtggc ggtggcgaag agcaagctcg agcagttcat gcgggatcac
     1261 gcgccggcgt gctgggccgc caaccccgac gcaccgaacc tgcaggtcac cgtccaggtc
     1321 tcgccggtcg gcgaggtcac gatcaaacag gcggccaatt cggcgatccg cgagtgcctg
     1381 ttcgagcgcg cgagcaagct gacgctcggc ccgagcgtcg acggcggcga attcaattat
     1441 cgattcgtcc gccccggcgg ttagcgtcag aaccggacgg agatccccgc agcgccgacg
     1501 cgcaggccga gcgggcgctg cggcggcgat ctgcggctcc cgacgaccag cagggcgacg
     1561 gtcgtcacga gcaacacggc ggtgaggctc gcgccggcga tcgccagccg gttgccgctg
     1621 gcgccccgcg cgtacacctc gtcgcggagc tgcagcagcg tgcctgcgtc gtgatgttga
     1681 tcggccgcct gttcggccct ggtcccgcgg gacacgccgg cgccgagcag caccgccgag
     1741 gccagcgtga gcccgccggt gaccccggct gcgatccaca gcggacgcgc cggattcgcc
     1801 gcggcggggg catggaccac ggcccgatcg ggcgtcgtcg cggccgggtc ggatcgcggc
     1861 gtgtcggttc gcggagcgcc cgggccgtcg agcagctcga tggctttcgg gcgctcgggt
     1921 cgcgccggcg cggccggctc ggggcaactg cgagtagcga gcgtctgctc gatcttctgc
     1981 agctccttct cggcgtactg cgccgacttc gggcgcgggt cggtggggat cgaggcgagg
     2041 tagtcctcga tcagcgcccg ggtccgacaa tgctgcgtgt gcagctcctc cggggaggcg
     2101 gtcgggacct gcgagacgtc gtcgagatag gcggcgatgc ggtcccagcg ggcgtcgtgc
     2161 ttgcggtcgc ggggcatgca gcgataggcc tcctcgaatt tgccgaccgc ctcggc
//
LOCUS       DASWNJ010000633         4747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_325440, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000633 DASWNJ010000000
VERSION     DASWNJ010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325440"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="VGB85_10610"
     CDS             <1..1049
                     /locus_tag="VGB85_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HEY0134526.1"
                     /translation="NVSIRVTLAGVPVPGALVQLAGLDQLLIWRPDELLAPDAKYELE
                     VAVDNEGLPADESFEGPCAPSLVVDRFTLTTSSELSEVPPLAPPTASIETYTVDQDFR
                     GVACCPGVVPQYGDPGGCHVSVFWEYSGAPNDCAYLYERTSLRYAAAAHAVAPAVGDQ
                     FVYQLVIDGEVVARSLAAPGLDVLHDMTACAHVEALHLGTGALVASPERCPSAEQAAI
                     IGPHPLDLAGAFGCAEPQVCGEDDGWGEACVPLDPQNLPPLSPPGPSPLEFGCPAAEV
                     ALDSSGDTEGPEIPEQGCKCTGGGLDSSVLGLALGFLVRRRRRRVSPRVTNVIIRSDR
                     GGPGASRFAGAADP"
     gene            1085..1555
                     /locus_tag="VGB85_10615"
     CDS             1085..1555
                     /locus_tag="VGB85_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134527.1"
                     /translation="MLLAALSACGPQPPAGTGSSTGEAETQGATPTSGEEPTTSGAHA
                     STGAVGMTSGTTSTSGPVASTGCDGACGESTGVAPDMGAPPACGVVLQDCPRGQECVP
                     DSFGTTCGRRVGVDIRPQAGDCGFERETIESRTGRSWTGASVAGTPDLKVEKRA"
     gene            1552..3042
                     /locus_tag="VGB85_10620"
     CDS             1552..3042
                     /locus_tag="VGB85_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134528.1"
                     /translation="MNFLRSHLSPTDPARWLSRRMALLAVGFAAACGGSGDADTECAT
                     TGQCTVTSVSSVSSVSNVSNVTNNTPTEDPTGAATGTGTGGPPTCEACGAEQVCVTGQ
                     CVDVPDQCPCPLETYCDLGLGKCVIGCTRDEECDEGRICDPIKRECAPGCREDADCGG
                     GQICEGLVCIAGCRTDATCGPMEICDAMSCRQGCNTDGECPAGQICDATVCRAGCTAD
                     ADCKTAGDICDPVKKVCRAGCYVDAECPLEKVCDAQQSCVPGCDSDAKCSAGKICTNG
                     QCVAGCNDDSGCSMGQICTNKLCVAGCKDNSGCSKGQICENMLCVAGCAVQEDCPLRQ
                     YCHEKKCIEGCGPPGGHKNEAVVDRCAVGEACYPQFCDVGYDCSKFECTPYCFGFPCK
                     SSANQTFDCISDFTPGYCMTQCDVNSDCSGGKVCALHAIPPEANHEDVGYCRSPCNSD
                     LNCKEMFWKGAYQASCTCSLAGALKGKCVKTENNVASECQHKDGGK"
     gene            3260..4180
                     /locus_tag="VGB85_10625"
     CDS             3260..4180
                     /locus_tag="VGB85_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134529.1"
                     /translation="MSRCLAFVPVALVLACATASPPPTPPEPQPVAERPPPSEPASPP
                     AGPGPVWPADATGLEVSDLGGGYGGLGRCHPNVARLDVATKQVSWEACDVVGFKGAQR
                     ATGARTLSDAEFAEVNAALQAVSRVEEPRCGVPDADRVHLRITTAAGVRTHASSLPDS
                     CPVEGIDSYVGDLAPIVKMLHRLGHHAQVEADCAAGKAESCTVAASHRLHLFSDIEGA
                     LAALDRGCKAGDLAACAQHATHDSGGAPVERLKAIERACDAKIAGACVTLADAYTEGK
                     YGAKKNGARAKSARARACAAGSQEECSAKP"
     gene            4480..>4747
                     /locus_tag="VGB85_10630"
     CDS             4480..>4747
                     /locus_tag="VGB85_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134530.1"
                     /translation="MQIKPGERTRPTPVVAAFPAVVVKVLTRELRDGACRAHAIEHAT
                     IWIARGDDSPSDGGVCNARGRVGHGARAVLHLDVLRRAGTVAARS"
BASE COUNT          728 a         1621 c         1690 g          708 t
ORIGIN      
        1 cgaacgtgtc gatccgcgtg acgctcgccg gggtgccggt cccgggcgcg ctggtgcagc
       61 tcgcggggct cgaccagctg ttgatctggc gcccggacga gctgctggcg cccgacgcga
      121 aatatgagct cgaggtcgcc gtggacaacg aagggctccc ggccgacgag agcttcgagg
      181 ggccgtgcgc cccgtccctg gtggtcgatc gcttcaccct gacgaccagc agcgagctga
      241 gcgaggtccc gcccctggcc ccgccgacag cgtcgatcga gacttacacg gtggaccagg
      301 attttcgcgg cgtcgcctgc tgccccggcg tcgtgcccca gtacggagat cccggtggct
      361 gtcatgtttc cgtcttctgg gagtacagcg gggcccccaa cgattgcgcg tacctgtatg
      421 agcggacctc gctgaggtac gccgccgccg cgcatgcggt cgcgccggcg gtcggtgacc
      481 agttcgtgta tcagctcgtc atcgatggcg aggtcgtggc ccgcagcctc gctgcgcccg
      541 gcctcgatgt gctgcacgac atgaccgcct gcgcccacgt cgaggcgctg cacctcggga
      601 ccggggcgct cgtcgcatcg cccgagcgct gcccgtccgc cgagcaggcc gccatcatcg
      661 ggccgcatcc gctcgacctc gccggggcgt tcggctgcgc cgagccgcag gtgtgcgggg
      721 aggacgacgg ctggggcgag gcatgcgtgc ccctcgaccc gcagaacctg ccgccgctct
      781 caccaccggg gccatcgccc ctcgagttcg gctgccccgc cgccgaggtc gcccttgatt
      841 cctcgggaga caccgagggg cccgagatcc ccgagcaagg ctgcaagtgt acgggcggag
      901 gcctcgacag cagtgtgctg ggcctcgcgc tcggcttcct cgtgcgccgt cgccgacggc
      961 gagtctcgcc gcgcgtgacg aacgtgatca tccgatccga tcgcggcggt cccggggctt
     1021 cccgctttgc gggcgccgcg gatccgtgat gtcatcgacc ctgaccatgc gatctatcgc
     1081 gtacgtgctg ctcgcggcgc tgtccgcctg cggcccacag ccgccggccg ggaccggcag
     1141 ctcgaccggc gaggcggaga cgcaaggagc gacgccgacg tcgggggagg agccgacgac
     1201 gagcggtgcc catgcgtcga ccggcgcggt ggggatgacg tccgggacga cgtcgacgag
     1261 cgggcccgtg gcctcgaccg gatgcgacgg cgcgtgcggc gagagcaccg gcgtggcgcc
     1321 ggacatgggc gcgccgccgg cctgtggcgt cgtgctgcaa gactgcccgc gcggccagga
     1381 gtgcgtcccg gatagcttcg gcaccacctg cggccgacgg gtcggcgtgg atatccgtcc
     1441 ccaggcgggc gattgtggat ttgagcgcga gacgatagag tcgcgaactg gccgaagttg
     1501 gacgggggct tcggttgccg gcacccctga cctgaaagtg gagaagcgag catgaatttt
     1561 ctgcgaagcc atctgtcacc gaccgacccc gcccgctggc tctcacgtcg gatggcgctg
     1621 ctcgccgtcg gtttcgcggc cgcgtgcggc ggctcgggtg acgccgatac cgagtgcgcg
     1681 acgacggggc agtgcacggt gacgagcgtc tcgagcgtct cgagcgtctc gaacgtctcg
     1741 aacgtcacga acaacacccc gaccgaggat ccgactggcg cggccacggg caccggtacc
     1801 ggtgggccgc ccacgtgcga ggcctgcggc gccgaacagg tgtgcgtcac cggtcaatgt
     1861 gtcgacgtgc ccgaccagtg cccgtgtccg ctagagacct actgcgacct cgggctcggc
     1921 aagtgcgtga tcggttgcac cagggacgag gagtgcgacg agggccgtat ttgcgacccg
     1981 atcaagcgcg agtgcgcgcc cggctgtcgc gaggacgccg attgcggggg tggccagatc
     2041 tgcgaggggc tggtgtgcat cgccggttgt cgcaccgacg ccacctgcgg cccgatggag
     2101 atctgcgacg ccatgtcctg tcgacagggc tgtaacaccg acggtgaatg tcccgccggc
     2161 cagatctgcg acgccaccgt gtgtcgcgcc ggttgcaccg ccgacgcgga ttgcaagact
     2221 gccggcgaca tctgcgatcc ggtcaagaag gtctgccggg ccggctgtta cgtcgacgcc
     2281 gaatgcccgc tggagaaggt ctgcgacgcc cagcagtcgt gcgtgcccgg ctgtgacagc
     2341 gacgccaagt gtagcgcagg caagatctgc accaatggac aatgcgtcgc cggctgcaac
     2401 gatgacagcg gctgctcgat ggggcagatc tgcaccaaca agctgtgcgt cgccggctgc
     2461 aaggacaaca gcggctgctc caaggggcag atctgcgaga atatgttgtg cgtcgccggc
     2521 tgcgccgtgc aggaggactg cccgctcagg cagtactgcc acgagaagaa gtgcatcgaa
     2581 ggttgtggcc cgcccggggg ccacaagaac gaagccgtgg tcgaccgctg cgcggtcggc
     2641 gaggcatgtt acccgcaatt ttgcgatgtc ggttacgact gctctaaatt cgagtgcacg
     2701 ccctattgtt tcggctttcc gtgcaagtcg agcgccaacc agaccttcga ctgcatcagc
     2761 gacttcactc ccgggtattg catgacgcag tgcgacgtca acagcgattg ctcgggcggc
     2821 aaggtgtgcg cgctgcatgc gatcccgccc gaggcgaacc acgaggatgt cggctactgc
     2881 cgctccccgt gcaacagcga tttgaattgc aaggagatgt tctggaaggg ggcatatcag
     2941 gcgtcctgca cctgctcgct cgcgggcgcg ctcaagggca agtgcgtcaa gacggagaac
     3001 aacgtcgcgt ccgaatgtca gcacaaggac ggaggaaagt gagccgtggc ccaccacggc
     3061 aggtacaagg ccaccaccag cagcgccggg gcccgcgagg tggccggtcg cgtcagtgtg
     3121 ggtacgctgc cgcaccttag aaaataggag gcgtcgagct cggcccgctc tggtctggcg
     3181 cggatcaggg gccctgctcg cccgcgcgca ggtctcctgg atctggcgaa ggcgcgggga
     3241 tcgccggtaa gatcggcgaa tgtctcgttg tctcgccttc gtgccggtcg cgctggtcct
     3301 tgcctgcgcg acggcctctc ctcccccgac gccgcccgaa ccccaacctg tcgccgaacg
     3361 gccgcccccg tcggagccgg catcgcctcc cgcaggcccc gggccggtct ggccggcgga
     3421 cgcgaccggg ctcgaggtct cggacctcgg cggcggctac ggcgggctgg gccggtgcca
     3481 cccgaacgtc gcccggctcg acgtcgcgac gaagcaggtg tcgtgggagg cctgcgacgt
     3541 cgtcgggttc aagggggccc agcgagcgac cggcgcgcgc acgctgtcgg acgccgagtt
     3601 cgccgaggtg aacgccgccc tgcaggccgt atcacgcgtc gaggagcctc gctgcggcgt
     3661 gcccgacgcc gatcgcgtgc atctgcgcat cacgacggcc gccggcgtcc gcacccacgc
     3721 cagctcgctg ccggatagct gtcccgtcga gggcatcgac agctacgtcg gcgacctcgc
     3781 gccgatcgta aagatgctgc accggctcgg ccaccacgca caggtcgagg ccgactgcgc
     3841 cgccggcaag gccgagagct gcaccgtcgc ggcctcgcac cggctccacc tgttctccga
     3901 catcgagggg gccctcgccg cactcgaccg gggctgcaag gcgggagacc tcgcggcctg
     3961 tgcccagcac gcgacgcacg acagcggcgg cgccccggtg gagcgactca aggcgatcga
     4021 gcgcgcctgc gacgcgaaga tcgccggcgc ctgcgtcacg ctcgcggacg cgtacaccga
     4081 gggcaagtac ggcgccaaga agaatggggc gcgggccaag tcggcgaggg ccagggcgtg
     4141 cgccgcgggc tcgcaggagg agtgctcggc gaagccgtga tcgggcccta tcggcccgcg
     4201 gacgcgacga tccggccgcg gccacgctat tcgacgagca cggcggtggc gtgccgcggc
     4261 ccgcatggcc gctgctcgcg tagatcacga cgtcgatcgg gccgggctcg ggtgcggtga
     4321 attgcgcctg ggcggcgtga ccgtgccgct ggcctcggtc accaccgcac cacatcacca
     4381 atattcggct ctccgaacgc cgccagggac gccaaggagg atcacagggc cggttcaatg
     4441 tgaaaccgtt ccacatcccc tgtaccgcag cgctgccagg tgcagatcaa gcccggggag
     4501 cggaccaggc cgacgccggt cgtcgctgcg tttcccgcgg tggtcgtcaa ggtcctgaca
     4561 cgcgagttac gcgacggcgc ctgccgtgcc catgcgatcg agcacgcgac aatatggatt
     4621 gccagggggg acgattcgcc ctctgacggc ggcgtgtgca acgcccgcgg tcgagtcggc
     4681 cacggagcac gcgctgttct gcatctggat gtcctccggc gtgcgggaac ggtcgccgct
     4741 cgttcgt
//
LOCUS       DASWNJ010000634         2438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_117530, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000634 DASWNJ010000000
VERSION     DASWNJ010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117530"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1470)
                     /locus_tag="VGB85_10635"
     CDS             complement(115..1470)
                     /locus_tag="VGB85_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134531.1"
                     /translation="MSRRLSSPASSPALLVSAASPVRGWFVRLVPLAVVALLGCADDK
                     LALGIDESSSSGDDTAADDTAADDTAADDTVTGDTAEPVVPDGEPIAELTSECELRFS
                     CDCAYDRFADIAACEEYYMVEWAGIVGQAQAAGLTADLECYVAALPHENYACDSYSEY
                     TEHHASDEPQACSYCQVAYGDRQVGESCQDFGEGVGDCARGLLCLNLEPGVSQCMDPC
                     SRAGVGESCAFVACEDGLACNWADGLCGVTGGDGGLCADGLCDDGLACHWGTNTCKQP
                     VGLGESCQDQPCGVGLLCDAEQVCIAPPGLGEACESFGCADGLVCQWDDLTCATPGQE
                     GEGCGNKDCASGLFCDVALMTCTPPGALGEPCDGRPCGDELACDWQTFTCAEIPELGE
                     PCSDVCATGLLCNPNASPAVCTPMPAEGEPCIWDTCAPNFVCDYNTQQCVEEPAIICG
                     M"
     gene            complement(1756..>2438)
                     /locus_tag="VGB85_10640"
     CDS             complement(1756..>2438)
                     /locus_tag="VGB85_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134532.1"
                     /translation="RRCRLTHRYVNYEAEPPNGRIFEADEKLVDKLVTTHTILATGAR
                     EGDTTITGDPAQAASPAGQALKDVHRFFCLRFPDEPIGVGAKWKDVCHLRTGGVVDTR
                     EVIWELTSMSKDPEGRARAEFTYLGRYTAPSASGERSGTVQGVLYYLLDAGEPHLLKE
                     QISTRRDPASNFYTTTTISYQFARWKKDKKGKPITVRTDGEPFPEAAPAPAAPAPAGK
                     AGDMTPAP"
BASE COUNT          343 a          808 c          934 g          353 t
ORIGIN      
        1 cgagggggcg ggacatggtc ggggctccgg gtgaccggcg ctggccggta cacctgatga
       61 gcgtcgcgat ccgcggtccc ggaccattac acgggcatta cggccgcgag cggttcacat
      121 gccgcaaatg atcgccggct cctcgacgca ctgctgcgtg ttgtagtcgc agacgaagtt
      181 gggggcgcag gtgtcccaga tgcacggctc gccctcggcg ggcatggggg tgcacacggc
      241 ggggctggcg ttcgggttgc acagcaggcc ggtcgcgcac acgtcggagc agggctcccc
      301 gagctccggg atctcggcgc aggtgaaggt ctgccagtcg cacgcgagct cgtcgccgca
      361 ggggcgaccg tcgcacggct cgccgagcgc ccccggaggg gtgcaggtca tgagcgcgac
      421 gtcgcagaag aggccggacg cgcagtcctt gttgccgcag ccctcgccct cctggcccgg
      481 cgtggcgcag gtcaggtcgt cccactggca cacgagcccg tccgcgcacc cgaaggactc
      541 gcaggcctcg cccaggcccg gcggcgcgat acagacctgc tcagcgtcgc agagcagccc
      601 gaccccgcac ggctggtcct ggcaagactc gccgagcccg acgggctgct tgcaggtgtt
      661 ggtcccccag tggcaggcga ggccgtcgtc gcacaggccg tcggcacaca gcccgccgtc
      721 accaccggtg acgccgcaga ggccgtcggc ccagttgcag gcgaggccgt cctcgcacgc
      781 gacgaaggcg cagctctcgc cgacgccggc gcgcgagcac gggtccatgc actgcgagac
      841 gcccggctcg aggttgaggc acaggaggcc ccgggcgcag tctccgacgc cctcgccgaa
      901 gtcctggcac gactcgccga cctgccggtc gccgtacgcg acctggcagt acgagcacgc
      961 ctgcggctca tccgaggcgt ggtgctcggt gtactcgctg tacgagtcgc aggcgtagtt
     1021 ctcgtggggc agcgccgcga cgtaacactc gaggtcggcg gtcagcccgg cggcctgcgc
     1081 ctggccgacg atgcccgccc actcgaccat gtagtactcc tcgcaggcgg cgatgtcggc
     1141 gaagcggtcg taggcgcagt cgcacgagaa gcgcagctcg cactcgctcg tcagctcggc
     1201 gatcggctcg ccgtcgggca cgaccggctc ggcggtgtcg ccggtgacgg tgtcgtcggc
     1261 ggcagtgtcg tcggcggcag tgtcgtcggc ggccgtgtcg tcgccggagc tcgaggactc
     1321 gtcgatgccg agcgcgagct tgtcgtctgc acagccgagc agggcgacga cggcgagcgg
     1381 gacgagacga acgaaccagc cgcgcacggg cgaggcggcg gagacgagga gagcggggga
     1441 cgaggcgggg gacgagagac ggcgagacat gacgggggct cctggtgacc ggcgaatgcg
     1501 gtacatccca tgagcagcgc gaccgtcggc ttcagatcat tacaggcgca ttacagcggc
     1561 gcgggcacac atgtgcgtgg tcgacagacg cgacgttcac tcgcggcgct gtcgagacgc
     1621 aggaccggat gaacgcgccg tcgtccccgc gaggcgcagc gccagcgcga gctcgctgcc
     1681 ggagctgcgc cggtcgatcg cgtgcgccgg ggtgtagagg tcggcgaagg cgtgggaccc
     1741 cgagtcggtc gtatctcagg gcgcaggcgt catgtcgccg gctttgccgg caggcgcggg
     1801 agcggcgggc gcaggcgcgg cctcggggaa cggctcgccg tcggtgcgca ccgtgatcgg
     1861 cttgcccttc ttgtccttct tccacctggc gaactgatac gagatcgtgg tcgtcgtgta
     1921 gaagttgctc gccggatcgc ggcgggtgct gatctgctcc ttgagcaggt gcggctcgcc
     1981 ggcgtcgagc aggtagtaca gcacgccctg cacggtgccg ctgcgctcgc cgctggcgct
     2041 cggggcggtg tagcggccca ggtaggtgaa ctcggcgcgg gcgcggccct cggggtcctt
     2101 gctcatgctg gtcagctccc agatgacctc gcgcgtgtcg acgacgccgc cggtgcgcag
     2161 gtgacagacg tccttccact tggcgccgac gccgatcggc tcgtcgggga agcgcaggca
     2221 aaagaagcgg tgcacgtcct tgagggcctg accggcaggc gaggcggcct gggcggggtc
     2281 gccggtgatc gtggtgtcgc cctcgcgcgc gccggtggcg aggatcgtgt gcgtggtgac
     2341 gagcttgtcg acgagcttct cgtcggcctc gaagatccgg ccgttcggcg gctcggcctc
     2401 gtagttgacg tagcggtggg tcaggcggca gcggcggg
//
LOCUS       DASWNJ010000635         1602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_153007, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000635 DASWNJ010000000
VERSION     DASWNJ010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153007"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..1254
                     /locus_tag="VGB85_10645"
     CDS             70..1254
                     /locus_tag="VGB85_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0134533.1"
                     /translation="MSNSILTPRILDRALGTVVGDLAIWRALEDSLEAFTTRLRGEPV
                     DEADEAGATRIYVQKLAEAGLLRWMVPAGAGGAAEKLSSTAICLIRQWLARESGALDN
                     AFVMQGLGSNPVTMAGPAALREKLLPRVARGAAICAFALTEPEAGSDIKNMRTTATPD
                     GDGVRLRGGKCFISNAGVADSYVVFAREADSPPERPRFGAFWLPGDAPGLTVTPTKVI
                     APHPIGVVDLADVRVPAEHRIGAYGDGLKIALANLEVFRVTVGAAALGLADRALSETV
                     AHLKTRVQFGQPLATQQGLQFALADVATDHVAAQLLVYRAAGARDEGRSAPEMAGMAK
                     MFATESAQRTIDRAVQSFGGRGVTVGEVPERLYREIRALRIYEGTTEVQKLVIARALL
                     GA"
     gene            complement(1255..>1602)
                     /locus_tag="VGB85_10650"
     CDS             complement(1255..>1602)
                     /locus_tag="VGB85_10650"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEY0134534.1"
                     /translation="DGRHFDELTTSVADLGATPSVLVDKRQQLQVLYAALQALPLDLQ
                     ILVELFYWEELPAGEIGTVLGVPEGTVRSRLRRARELLGERLQTLSPSLAEPTLADLE
                     RWARELRDRIGRS"
BASE COUNT          229 a          512 c          633 g          228 t
ORIGIN      
        1 cggatttcca ggaggccgac cgcgcgcggc aggacaagcg gccgccgcgc tggcgctaaa
       61 gggggttgta tgagcaattc gatcctcacg ccgcggatcc tcgatcgtgc gctcggtacg
      121 gtggtcggcg acctcgcgat ctggcgcgcg ctcgaggatt ctctggaagc gttcacgacg
      181 aggctgcgtg gcgagccggt cgacgaggcc gacgaggccg gggcgacgcg gatctacgtg
      241 cagaagctgg ccgaggccgg gctgttgcgc tggatggttc cggccggagc tggtggcgcg
      301 gccgagaagc tgtcgtcgac tgcgatctgt ctgataaggc agtggctcgc gcgcgagagc
      361 ggggcgctgg acaacgcgtt cgtgatgcag ggcctcggct ccaacccggt gacgatggcc
      421 ggacccgcgg cgctgcgtga aaaattactg ccacgcgtcg cccgcggtgc ggcgatctgc
      481 gcgttcgcgt tgaccgagcc cgaggccggc tccgacatca agaacatgcg cacgacggcg
      541 acgcccgacg gcgacggcgt gcgcctgcgc ggcggcaagt gcttcatcag caacgccggg
      601 gtcgccgaca gctacgtggt gttcgcccgc gaggccgaca gcccgcccga gcgcccgcgc
      661 ttcggcgcgt tctggttgcc cggtgatgca cccggcctga cggtgacgcc gacgaaggtc
      721 atcgcgccgc acccgatcgg cgtggtcgac ctcgcggatg tccgggtgcc cgcggagcac
      781 cgcatcggcg cgtacggtga tggcctgaag atcgcgctgg cgaacctcga ggtgttccgc
      841 gtgacggtcg gcgccgcggc gctcggcctg gccgaccgcg cgctgtcgga gacggtcgcg
      901 cacctcaaga cgcgcgtgca gttcgggcaa ccgctggcga cccagcaggg gctgcagttc
      961 gcgctcgcgg atgtcgcgac ggaccacgtc gcggcgcagc tgctggtgta tcgggccgcg
     1021 ggtgcgcgtg acgagggccg cagcgcgccc gagatggccg ggatggccaa gatgttcgcg
     1081 accgagtcgg cgcagcgcac gatcgaccgc gcggtgcaga gcttcggcgg ccgcggtgtg
     1141 accgtcggcg aggtgcccga gcgactttat agagagatcc gggcgctgcg aatttatgag
     1201 ggcacgaccg aggtgcagaa gctggtcatc gcgcgcgcgc tgctcggggc atagtcagct
     1261 gcggccgatg cggtcgcgca gctcccgggc ccagcgctcg aggtcggcga gggtcggctc
     1321 ggcgagcgac gggctgaggg tctggaggcg ctcgccgagc agctcgcggg cgcggcggag
     1381 gcggctgcgg acggtgccct cgggcacgcc gagcacggtg ccgatctctc cggcgggcag
     1441 ctcctcccag tagaacagct cgacgaggat ctggaggtcg agcggaaggg cctgcaaggc
     1501 ggcgtagagg acctgcagct gctggcgctt gtcgaccagg acgctgggcg tggcgccgag
     1561 gtcggcgacg gaggtggtca gctcgtcgaa gtggcggccg tc
//
LOCUS       DASWNJ010000636         1832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_260026, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000636 DASWNJ010000000
VERSION     DASWNJ010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260026"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="VGB85_10655"
     CDS             <1..952
                     /locus_tag="VGB85_10655"
                     /inference="COORDINATES: protein motif:HMM:NF028299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system domain-containing
                     protein"
                     /protein_id="HEY0134535.1"
                     /translation="VSQVAAPASAAAAPTGDVANSAAVDRFLRSTGEELVKLANALRR
                     ARSADPLAYRLLRSGLWIYIAAPPPNQPDGSTGIPGLGEKDRETLAAMHANKRWPELL
                     ETSESLLSRARFALDLHRYSFDALQNLGESHEAGRLALTAEVGALLRRLPRVLALKDN
                     EGVPLADDATRAWIEASVLPSGGPASSSTTVVTVAAGPPGAEAAELRALFTANKREEA
                     LRLGAAQIQQAGSGREKFLRRLDLAEACDPILARTLFSGLVAEVEAQRLDTWEPALAV
                     RCYEGLARTIPRGQASDKPALDAALVRLAGLDPARAAAIK"
     gene            complement(1241..>1832)
                     /gene="tssD"
                     /locus_tag="VGB85_10660"
     CDS             complement(1241..>1832)
                     /gene="tssD"
                     /locus_tag="VGB85_10660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HEY0134536.1"
                     /translation="RVFATGTIERREKTSNCGDHTVYSARGPSLMVHGMAESIHLYLK
                     ANGQDIQGESTQHSNGRENSIECVEFKDNVRTAREQGSRAAVGRRVHEPIAIIKRIDK
                     STPLLAKALCNNERIEGEFKFFRPNPAGDGTTEQFFTVKIEDARIDSISRHSPNVTDP
                     AESKAPPLETVTFVYAKITWTYVPTGAEHVDTWANS"
BASE COUNT          249 a          660 c          634 g          289 t
ORIGIN      
        1 cgtctcgcaa gtcgcggcgc cggcgtccgc cgccgcggcc ccgaccggcg acgtcgccaa
       61 cagcgcggcc gtcgatcgct tcctgcggag caccggcgag gagctggtca agctggccaa
      121 cgccctgcgt cgcgctcgct cggccgaccc gctcgcgtat cgcctgctgc gctcgggcct
      181 gtggatctac atcgcagcgc cgccgccgaa ccaacccgac ggcagcaccg ggatccccgg
      241 gctcggcgag aaagatcgcg agaccctcgc ggccatgcac gccaacaagc gctggcccga
      301 gctgctcgag accagcgagt cgctgctgtc cagagcgcgc ttcgccctcg acctccaccg
      361 ctacagcttc gacgcgctgc agaacctcgg cgagtcgcac gaggccggtc gcctggcgct
      421 cacggcggag gtcggcgcgc tgctccggcg cctgccgcgg gtgctcgccc tcaaggacaa
      481 cgagggcgtg ccgctcgccg acgacgccac ccgcgcgtgg atcgaggcgt cggtgctgcc
      541 gagcggcggg ccggcgagca gcagcacgac ggtggtgacc gtcgccgcgg ggccgcccgg
      601 ggccgaggcc gcggagctgc gggcgctgtt caccgccaac aagcgcgagg aggcgttgcg
      661 cctcggcgcc gcccagatcc agcaggccgg cagcggacgc gagaagttcc tgcggcgcct
      721 cgacctcgcc gaggcctgcg acccgatcct cgcgcgcact ctattttcgg ggctcgtcgc
      781 cgaggtcgag gcgcagcgcc tcgacacctg ggagcccgcg ctcgcggtgc gctgctacga
      841 gggcctcgcg cgcacgatcc cccgcggaca ggcgagcgac aagccggccc tcgacgcggc
      901 gctcgtgcgt ctcgcgggcc tcgacccggc acgcgcggcg gccatcaagt gacccgcgga
      961 tcggcgccgc gttgcctatg catcgcggtc gccgattaaa cgccgcgggc cacggtgggc
     1021 gagcgcgcgt acgcgtccgt cccagcgcgg cccaggtcgc tcgtcgagcg tatcctcgcc
     1081 atttgacacg gccaggtcat tgatcgcggc cgacataaag gtcggccgct gtctcatcat
     1141 gctcctcgcc tggtggccag gccccctgct gcgcccgggc attccgtggc gtggcggggg
     1201 tcctgcgcgt ccggcgggcg cgcagcgctc gtgcggacga tcagctgttg gcccacgtat
     1261 cgacgtgctc ggcgcccgtc ggcacgtacg tccacgtgat cttcgcgtac acgaaggtca
     1321 ccgtctcgag cggcggggcc ttcgactccg cggggtccgt gacgttgggc gagtgacgcg
     1381 agatggagtc gatgcgggcg tcctcgatct tgaccgtgaa gaactgctcg gtggtgccat
     1441 caccagccgg gttcggacgg aagaacttga actccccctc gatcctctcg ttgttgcaca
     1501 acgccttcgc cagcagcggc gtgctcttgt cgatgcgctt gatgatcgcg atcggctcgt
     1561 gcacgcggcg accgacagcg gcgcggctgc cctgctcacg ggcggtgcgg acgttgtcct
     1621 tgaactctac gcattcgata gagttctcgc gaccgttgct gtgctgggtg ctctcaccct
     1681 ggatgtcttg gccattggct ttcaggtaca ggtggattga ctctgccatg ccgtgaacca
     1741 taagcgacgg tccgcgggcc gaatagaccg tgtgatcgcc gcagttcgag gtcttttcgc
     1801 gccgctcgat tgtgccagtg gcgaacacgc gc
//
LOCUS       DASWNJ010000637         8091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_436640, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000637 DASWNJ010000000
VERSION     DASWNJ010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 8091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8091
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436640"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VGB85_10665"
     CDS             complement(<1..831)
                     /locus_tag="VGB85_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY0134537.1"
                     /translation="MHSAWSDADAAAAITRYTTAYPGTCNEDVALRVYTSQLLGRSAG
                     LVLHGGGNTSVKTTLPDDLGEATAVACIKGSGWDLANIEPPGLPAVRLAPLLALRRLP
                     SLSDEAMVTALRARLLDPAAPNPSVEALLHAFLPHKYIDHSHADAVLAVADQPRALEL
                     CAEVFGDDYVAVPYVMPGFTLALRAAEAYEQAPECHGLVLLGHGLFSFGASARESYER
                     HIAAVTRAEEFLARGRKSFVAGASADEAAFLRLAPVLRGRLKSARVLHWRPVPEALSF
                     A"
     gene            complement(888..1580)
                     /locus_tag="VGB85_10670"
     CDS             complement(888..1580)
                     /locus_tag="VGB85_10670"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0134538.1"
                     /translation="MSRLLDVSTSFVSTLLRPGAGMIVRGVGARPEARIRLYEFEGCP
                     YCRKVREMLTTLDLDAAIFPCPKGGTRFRSEVEKHGKQQFPFMIDPNTNTELFESDDI
                     IKYLAERYGDGKVPLSLRMGPLTVLSGSAGSLIRFPRGTRARPSRMPEGPLELYSYEA
                     SPYSRLVREALSELELPYVLHNLGRGSRKREEFVKLSGKMQVPFLVDPNTGVAMFESA
                     EIVKYLDQTYAL"
     gene            1931..4105
                     /locus_tag="VGB85_10675"
     CDS             1931..4105
                     /locus_tag="VGB85_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0134539.1"
                     /translation="MLSALRVILEYLRPYRTEVWLLLFGLLIDLAWAVLFGMSFQYVV
                     DEAIAKKDEALLWTVLIGLVAAALLAAMAAIGRDYLYARLGTSVMNDLRDRMFAHLQR
                     LSAGYYSKVQIGDIMSRFSTDLAAVQNAVILAIPETILGTLGLITISTLLFIFSWQLA
                     LVTVLGLPLAMLGPRFLGPRAERDSYLVRHEEAKIASTVQENVSAQPVIRAFGLAPLQ
                     LGAFRSQLKGFYKISLRFNVLAYLVERTPNLSFLLLQIAVFGFGAVKCFEGELSLGSL
                     IAFNVLIGYLSAYVTSLTRVMPPLLLAAGGVQRIQELLGTQPQVAEVAGAAPLPVFAD
                     ALAFADVSFSYTGEHKNLDSVSFTVRRGAQVAIVGGSGSGKSTALGLIARFYDPDTGA
                     VTIDGRDLRTASLTALRGQMGVVFQESFLFNTTIRENIRYGRPDASDAEVEAAARAAE
                     IHELVVSLPDGYNTIVGERGGRLSGGQRQRVAIARAIVRDPAILLLDEATSALDPTTE
                     AAVNGTLERLGRERTVISVTHRLASTVSADQILVFERGRLVEQGTSSELLAQGGMYKR
                     LWDKQQGLVISEDGARAGIEPERLRVVPVLSKLEDAMLEKLAKMFLSERVSEDRKVIR
                     EGDTTVDKFYIIARGKVAVLKRQPDGTDVQVATLQDGDHFGEVALLKDAPRNATIRTL
                     TPCIFLTLQRRQFMELIQSDPRLRSQFTTVSELRGTVPQTLV"
     gene            4165..4713
                     /locus_tag="VGB85_10680"
     CDS             4165..4713
                     /locus_tag="VGB85_10680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HEY0134540.1"
                     /translation="MDAVISPLLRARTWATPKIELRQRCLLSADFGVIACEAIPAGSV
                     LMSLAHVFVDRPGRHTIQIDARRHQAGTGEIDDYLNHSCDPNSALDFTRLELVSLRKI
                     APGEELSFNYLTSEWDMAAPFACQCGSCDRLGGRPGPRLIRGFRHLTSVEQDLLAPLA
                     SPYLRQRLAELRTTTPRRRLAG"
     gene            4838..5797
                     /locus_tag="VGB85_10685"
     CDS             4838..5797
                     /locus_tag="VGB85_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0134541.1"
                     /translation="MTIRDSAALLLSLGIALAPLRVEAAPATGSASTGSAGTTSPTGT
                     TNSTGSAGASTPAPAATSPTAGDDKAANLKRAEELFRNGERLYTEGSYESAILAFQEC
                     YELSKEPQLLYNIGNAYERLGDFANSRRYLDQYRAFAPEKEREALSRRIQALDQRQRE
                     KEARDRENAEKNAASSGGTTGAQPATGPQPVSPPADTAPPKKDRMFGPAAIALTAGVA
                     LGLGLGIGFGATATKQKQSALDGCSDVDGDLYCSADSKDFLAKRKLNALVADIGFAVA
                     GAAAIGLVTVLVLKARKNKTQGRAQLAPYAGPRGAGMTFNLRF"
     gene            complement(5854..6267)
                     /locus_tag="VGB85_10690"
     CDS             complement(5854..6267)
                     /locus_tag="VGB85_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134542.1"
                     /translation="MVAVERDGIKYLSLPALIELKLASGMTSSERIKDLADVQELIKI
                     CKLPRDFKAQLNPFVHEKFDALWDAAMGHARRFMMILHGASPDAAAALEAMLADGVTV
                     DPDGRNGDRIRLTTTDPDVAARYGMHDESEFFDEP"
     gene            complement(6327..6611)
                     /locus_tag="VGB85_10695"
     CDS             complement(6327..6611)
                     /locus_tag="VGB85_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134543.1"
                     /translation="MNEGSRHFEERGGVAQALRKIVARLEELGIAYVIVGGMSLFAHG
                     LRRFTEVVDILVKMNDLRTIHSKLVGLGYRRVFEDRRIYATRSSGSGSSS"
     gene            complement(6720..>8091)
                     /locus_tag="VGB85_10700"
     CDS             complement(6720..>8091)
                     /locus_tag="VGB85_10700"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0134544.1"
                     /translation="KVLSPELSVRPDHVDRFMREARSASMINHPNVVEITDFGVTPDG
                     QPFFVMEYLQGKDLSQVIGESGSLPWKRVRPIALQICHALQAAHDQGVIHRDMKPGNV
                     VLVKRGNTAEHVKVLDFGIAKVLGEAEAKGLTQSGTVVGTPEYMSPEQGWGQPVDHRG
                     DIYALGVMIYELLTGKIPFSGTTMMEILNRHMFEVPDIKMPNIPEEVGAIILKAMQKD
                     RALRFQSMNELGAAIEAVGTGASPVTVVDEEIKTPWGPVTARFSTLPPQAAAPPRSWV
                     WLSVLSAVIAVGALVFALLPDAPPAPVAPVPQVVVAPEPVEPVAPEPKPVVLPAEVEK
                     VMFTITTPGVDAQILDARDEGIYGMTNDPKGVLVRKSEDPLELILRAKGHEDERFTII
                     PDQDKRFEKPLTPAKAVAPKDKPRPKHDTRPKTDPKPATDTKTKPPENDDTGLGTDLK
                     NPFGKK"
BASE COUNT         1241 a         2916 c         2657 g         1277 t
ORIGIN      
        1 cgcaaaggac agggcctccg ggacaggtcg ccagtgcagc acccgcgcgc tcttcaaacg
       61 cccgcgcagc accggcgcca ggcgcaggaa cgccgcctcg tcggcgctcg cacccgctac
      121 aaaactcttt cgcccgcgcg ccaggaattc ctcggcccgc gtcaccgcgg cgatgtgtcg
      181 ctcgtagctc tcgcgcgcgc tggccccgaa gctgaacagt ccgtgtccca gcagcaccag
      241 cccgtgacac tccggcgcct gctcgtacgc ctctgcggct ctcagcgcga gcgtgaagcc
      301 cggcatcacg tatggcaccg cgacatagtc atctccaaac acctcggcgc acagctcgag
      361 cgcccgcggc tgatctgcca ccgcgagcac cgcgtcggcg tgcgagtggt cgatgtactt
      421 gtgcggcaaa aacgcgtgca gcagcgcctc caccgagggg ttgggcgcgg cggggtccag
      481 caggcgcgcg cgcagagccg tcaccatcgc ctcgtcactc aacgacggca gccggcgcag
      541 ggccagcagc ggggccaggc gcacggccgg cagcccgggc ggctcgatgt tcgccaggtc
      601 ccagccgctg cccttgatgc acgcgaccgc ggtcgcctcg ccgaggtcgt cgggcagcgt
      661 cgtcttgacc gaggtattac cgccgccgtg cagcaccagc ccggcagagc gcccgagcaa
      721 ctgcgacgtg tacacgcgca gcgcgacgtc ctcgttgcag gtgcccgggt aggcggtggt
      781 atagcgggtg atggcggccg cggcgtcggc gtcggaccag gcgctgtgca tggcgcccac
      841 cttactggcg gcgggcgccg gaggccagcg ccctcacctc gctgtaatca cagcgcgtag
      901 gtctggtcga ggtacttgac gatctcggcc gactcgaaca tcgcgacccc ggtgttcggg
      961 tcgacgagga acggcacctg catcttgccg gacagcttca cgaattcctc gcgcttgcgg
     1021 ctgccgcggc cgaggttgtg gagcacgtag ggcagctcga gctcgctgag cgcctcgcgg
     1081 accaggcgcg agtagggcga ggcctcgtag ctgtacaact cgagcggccc ctcgggcatg
     1141 cgcgacggcc gggcccgggt cccgcgcggg aaccggatca gcgagccggc ggagcccgac
     1201 agcaccgtca gcggacccat gcgcagcgac agcggcacct tgccgtcgcc gtagcgctcg
     1261 gccaggtact tgatgatgtc gtcggactcg aacagctccg tgttggtgtt cgggtcgatc
     1321 atgaagggga actgctgctt gccgtgcttc tcgacctcgg accggaagcg ggtgccgccc
     1381 ttggggcacg ggaagatcgc cgcgtcgagg tcgagggtcg tcagcatctc gcggaccttg
     1441 cggcaatacg ggcagccctc gaactcgtag agacggatcc gcgcctcggg gcgggctccc
     1501 acgccgcgga cgatcatgcc ggcgccgggg cgcaggaggg tggacacgaa cgaggtggag
     1561 acatcgagca ggcgcgacat gggccaccct gtagcgcgag ccagccgccc cggccaccac
     1621 gagccggacg atcgcccgcg tcgcggccgt gaacccgcag gaacgccgca cgcccggccc
     1681 gcgtcgaccg cagaggccgg ctgcggtggc ctcacgtacc gcgcccgcgg ctcgactgcg
     1741 cggactccga gcgctcgccc acgttccctg tattaccaac ctgacaaaag ccatgcgggc
     1801 cgccagaggc ccgtggagcg acctgtgacg gtcagcgact ccccgggcgt ccacggcttg
     1861 tgtcaccatg cggagcctga ccgcgggcgc cgcctgcgaa accgcgcgaa gcgccgctct
     1921 cgacccgctc atgctcagcg ccctccgagt catcctcgag tatctccggc cgtaccggac
     1981 cgaggtgtgg ctgctgctgt tcggcctgct gatcgacctc gcgtgggcgg tgctgttcgg
     2041 gatgagcttt cagtacgtcg tcgacgaggc gatcgccaag aaggacgagg cgctgctgtg
     2101 gaccgtgctg atcggcctgg tcgccgcggc gctgctcgcc gcgatggcgg cgatcggccg
     2161 cgactacctc tacgcgcggc tcggcaccag cgtgatgaac gacctgcgcg accgcatgtt
     2221 cgcgcacctt cagcgcctgt cggccggcta ctactccaag gtccagatcg gcgacatcat
     2281 gtcgcgcttc tcgaccgacc tcgccgccgt ccagaacgcg gtgatcctgg ccattcccga
     2341 gacgatcctc ggcaccctcg gcctcatcac gatctcgacc ctgctgttca tcttctcctg
     2401 gcagctcgcg ctcgtgaccg tgctcggcct gccgctggcg atgctcggcc cgcgattttt
     2461 gggcccgcgc gccgagcggg acagctacct cgtacgccac gaggaggcca agatcgccag
     2521 caccgtccag gagaacgtca gcgcccagcc ggtcatccgc gcctttggcc tcgcgccgct
     2581 ccagctcggc gcctttcgct cccagctcaa gggtttctac aagatcagcc tgcgcttcaa
     2641 cgtgctcgcc tacctcgtcg agcgcacccc gaacctgtcc tttctgctcc tgcagatcgc
     2701 cgtgttcggc ttcggcgcgg tcaagtgctt cgagggcgag ctgtcgctcg gctcgctgat
     2761 cgccttcaac gtgctgatcg gctacctcag cgcctacgtc acctcgctga cccgcgtcat
     2821 gccgccgctg ctgctcgccg ccggcggcgt gcagcggatc caggagctgc tcggtaccca
     2881 gccgcaggtc gccgaggtcg ccggcgccgc gccgctgccg gtcttcgccg acgcgctcgc
     2941 gttcgccgac gtgtcgttct cctacaccgg cgagcacaag aacctcgaca gcgtcagctt
     3001 caccgtccgc cgcggcgcgc aggtggcgat cgtcggcggc agcggctccg gcaagagcac
     3061 cgcgctcggc ctgatcgccc gcttctacga ccccgacacc ggcgcggtca cgatcgacgg
     3121 ccgcgacctg cggaccgcct cgctcaccgc gctgcgcggg cagatgggcg tcgtgttcca
     3181 ggagagcttc ctgttcaaca cgacgatccg cgagaacatc cgctacggcc gccccgacgc
     3241 gagcgacgcg gaggtcgagg ccgccgcccg ggccgccgag atccacgagc tcgtcgtctc
     3301 gctgcccgac ggctacaaca cgatcgtcgg cgagcgaggt gggcgactgt cgggtggtca
     3361 gcgccagcgc gtggcgatcg cccgcgcgat cgtgcgtgac ccggcgatct tgctgctcga
     3421 cgaggcgacc tccgccctcg acccgaccac cgaggccgcc gtcaatggca cgctcgagcg
     3481 cctcggtcgc gagcggaccg tcatttcggt cacccaccgc ctcgcctcga ccgtcagcgc
     3541 cgaccagatc ctcgtgttcg agcgcgggcg gctggtcgaa caggggacga gcagcgagct
     3601 gctcgcacaa ggtggcatgt acaagcgcct gtgggacaag cagcagggcc tcgtcatcag
     3661 cgaggacggt gcccgcgcag gtatcgagcc agagcggcta cgtgtcgtac ctgtgctgtc
     3721 caagctcgag gacgccatgc tcgagaagct ggcaaagatg ttcctgagtg agcgggtatc
     3781 ggaggatcgc aaggtcattc gcgagggcga taccaccgtt gacaagttct acatcatcgc
     3841 acgtggcaag gtcgccgtgc tcaagcgcca acccgatggt acggatgtac aagtggccac
     3901 gctgcaagac ggtgatcact tcggagaggt ggcgttgctc aaggacgcgc cgcgtaacgc
     3961 gacgatccgc acgctgacgc catgcatctt cctgaccctg caacgccgcc aattcatgga
     4021 gctcatccag agtgacccgc ggctgcgcag tcagttcacc accgtctccg agctgcgcgg
     4081 caccgtccca caaaccctcg tttagccctc gtgtagccct cgtttagccc tcgtgtagct
     4141 ctcgcccaga gcaggaccac caccatggac gctgtcatat ctccgctgct ccgcgcccgc
     4201 acctgggcga ccccgaagat cgagttacgt cagcgctgcc tgttgtccgc cgacttcggc
     4261 gtgatcgcct gcgaggcgat ccccgccggg agcgtgctga tgtcgctcgc acacgtgttc
     4321 gtcgaccgcc cgggacggca caccatccag atcgacgccc gccgccatca ggccggcacc
     4381 ggcgagatcg acgactacct caaccacagc tgcgacccca acagcgccct cgacttcacc
     4441 cgcctcgagc tggtctccct gcgaaagatc gccccgggtg aggagctcag cttcaactac
     4501 ctcacgtccg agtgggacat ggcggccccc ttcgcctgtc agtgcggctc ctgcgaccgc
     4561 ctcggcggca gaccagggcc ccgcctcatc cgcggctttc gtcacctgac ctcggtcgag
     4621 caggacctgc tggcccccct cgcctcgcct tacctgcgcc agcgcctcgc cgagctgcga
     4681 accaccaccc cgcgtcgccg cctggccggc tgagcccggt cgggtggcgc ctgaccgaag
     4741 ggccagccga ggtcgcgcgc caggcttgcc tccgcgcctc ccgggcccct atactgccgc
     4801 cattcgcggc attcccaggg atcccgccca ggttcgcatg accattcgag acagcgcagc
     4861 cctcctcctg agcctcggca tcgcccttgc gccgctccgc gtggaggctg ctccggccac
     4921 cggctccgcg tccactggtt cggccggcac gaccagcccg accggcacga ccaactcgac
     4981 cggttcggcc ggcgcgagca cgcccgcccc ggcggcgacg agcccgaccg cgggcgacga
     5041 caaggcggcg aacctcaagc gagccgagga gctgttccgc aacggcgagc gcctgtacac
     5101 cgagggctcc tacgagtcgg cgatcctcgc gttccaggag tgctatgagc tgtcgaagga
     5161 gccgcagctg ctctacaaca tcggcaacgc gtacgagcgc ctcggcgact tcgccaactc
     5221 gcgccgctac ctcgatcagt accgggcgtt cgccccggag aaggagcgcg aggccctgtc
     5281 gcggcggatc caggcgctcg accagcggca gcgggagaaa gaggccaggg accgcgagaa
     5341 cgcggagaag aacgccgcca gctcgggcgg gaccacgggc gcgcagccgg ccacgggccc
     5401 gcagccggtc tcgccgccgg ccgacacggc cccgcccaag aaggaccgca tgttcgggcc
     5461 cgccgcgatc gcgctgaccg ccggcgtcgc tctcgggctc ggactcggca tcggcttcgg
     5521 ggcgaccgcg accaagcaga agcagagcgc cctcgacggc tgctcggacg tcgacggcga
     5581 cctgtactgc tcggccgaca gcaaggactt cctcgccaag cgcaagctca acgccctcgt
     5641 cgccgacatc ggcttcgcgg tcgccggcgc ggccgcgatc ggcctcgtca ccgtgctcgt
     5701 gctcaaggcc cgcaagaaca agacccaggg ccgcgcccag ctcgccccct acgccggccc
     5761 acggggcgcc ggcatgacct tcaaccttcg cttctagttc gcttttcggc gaagccgtcg
     5821 ccgctcacag cggcgcgagc acccgcgcga gcgtcagggt tcgtcgaaga actcgctctc
     5881 gtcgtgcata ccgtacctgg ctgccacgtc ggggtccgtc gtggtcaggc ggatgcggtc
     5941 cccgtttcga ccgtcgggat ccaccgtaac gccgtcggct agcatcgctt ccagcgccgc
     6001 ggcggcgtcc ggagaggcac cgtgcaggat catcatgaag cgtcttgcgt ggcccatggc
     6061 cgcgtcccaa agcgcatcga acttctcatg gacaaagggg ttgagctggg ccttgaagtc
     6121 tcgcgggagc ttgcagatct tgatcagctc ctggacatcc gcgagatctt tgatccgctc
     6181 ggagctggtc atgcctgacg cgagcttgag ctcgatcaaa gccggcaatg agagatattt
     6241 gatcccgtca cgctcgacgg cgaccatgct cggatcagga aactctaccg gcttgggctt
     6301 gccgtccccg ggatactgac cagcgatcag gaactcgatc cggaccccga gctccgcgtc
     6361 gcgtagattc ttcgatcctc gaacacgcga cggtaaccca ggccgaccag cttgctgtgg
     6421 atcgtccgca ggtcgttcat cttgacgagg atatcgacaa cctcggtgaa gcggcgcagc
     6481 ccatgggcga acagcgacat gccaccgacg atcacgtacg cgatcccgag ctcctcgagc
     6541 cgcgcaacga tcttgcgcag cgcctgcgcg accccgccac gctcctcgaa gtgcctactg
     6601 ccctcgttca acgcccacct cgggtctgcg gcgagttggc cctcataact cagcccgggg
     6661 ttgtccgagg catgccgatc aggcccgcgg tccagtgagg acgacatgac cccgcgcgct
     6721 cacttcttgc cgaaggggtt cttgaggtcg gtgccgaggc cggtgtcgtc gttctcgggc
     6781 ggcttggtct tggtgtcggt ggccggcttg gggtcggtct tcggcctggt gtcgtgcttg
     6841 gggcgcggct tgtccttcgg cgcgacggcc ttggccggcg tcagcggctt ctcgaagcgc
     6901 ttgtcctggt cggggatgat cgtgaagcgc tcgtcctcgt ggcccttggc gcgcaggatc
     6961 agctcgagcg ggtcctcgga cttgcggacc agcacgccct tggggtcgtt ggtcatgccg
     7021 tagatgccct cgtcgcgggc gtcgaggatc tgcgcgtcga cgccgggcgt ggtgatcgtg
     7081 aacatcacct tctcgacctc ggccggcagc accaccggct tgggctcggg cgccaccggc
     7141 tcgaccggct cgggcgcgac gaccacctgt ggcacggggg cgaccggggc cgggggcgcg
     7201 tcgggcagca gcgcgaacac cagcgcgccg acggcgatca ccgccgagag cacgctgagc
     7261 cacacccagc tgcgaggcgg cgccgcggcc tggggaggca gggtcgagaa gcgggcggtg
     7321 acggggcccc agggggtctt gatctcctcg tcgacgacgg tgaccgggct ggcgccggtg
     7381 ccgacggctt cgatcgcggc gccgagctcg ttcatcgact ggaagcgcag cgcccggtcc
     7441 ttttgcatcg ccttgaggat gatcgcgccg acctcctcgg ggatgttcgg catcttgatg
     7501 tcgggcacct cgaacatgtg ccggttcaag atctccatca tcgtcgtgcc ggagaacggg
     7561 atcttgccgg tcagcagctc gtagatcatc acgccgaggg catagatgtc gccgcggtgg
     7621 tcgaccggct ggccccagcc ctgctcgggc gacatgtact cgggggtgcc gacgacggtg
     7681 ccgctctgcg tcaggccctt ggcctcggcc tcgccgagca ccttggcgat gccgaagtcg
     7741 agcaccttga cgtgctccgc agtgttgccg cgcttgacca gcacgacgtt gcccggcttc
     7801 atgtcgcggt gaatgacgcc ctggtcgtgc gcggcctgca gggcgtggca gatctgcaag
     7861 gcgatcgggc gcacgcgctt ccatggcagc gagcccgact cgccgatcac ctgcgacagg
     7921 tccttgccct gcaggtactc catcacgaaa aatggctggc cgtcgggggt cacgccgaag
     7981 tcggtgatct cgaccacatt cgggtggttg atcatcgagg ccgacctcgc ctcgcgcatg
     8041 aagcggtcga cgtggtccgg gcgcaccgac agctccgggc tcaggacctt g
//
LOCUS       DASWNJ010000638         2642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_223914, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000638 DASWNJ010000000
VERSION     DASWNJ010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223914"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VGB85_10705"
     CDS             <1..253
                     /locus_tag="VGB85_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134545.1"
                     /translation="LAVAIAPAAVAGWSGIVAAKVGLLVISLALFIYVSWVLWPLRVF
                     ASTDELPAVRRRFWWVGVVMIACNALNMGLGILAHALRG"
     gene            complement(269..>2642)
                     /locus_tag="VGB85_10710"
     CDS             complement(269..>2642)
                     /locus_tag="VGB85_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134546.1"
                     /translation="RPAQATSPRVNTDNEPTRPLPAARAPEEPTRIRPLRGRRQVIAL
                     VNPIRRDTGDTTSDAHRARRDAPDELPPATAWLAKPDAAPSRVEPVLEDSTPPASREQ
                     VSDAASEPRRTSDEAAAGSTASEQPVPRDTHEPRAAESAVPTAAQDSATSDGSVPADT
                     HDSADAATDAPVAAEPVPADTRDARDAAAAEPVPEDTRDTPDPADASEATREPLAAEP
                     APGDTHDAAAEPAPQDSSSSELTPADPADASAVLHLPNLVAPPGARRAVGKKGDRLRT
                     GDAALVMPDLRRPGASASTGGASESGTTLSIPASLIAPTPLPGARRRGSKTGQNPVVP
                     GADEITLNMPRLVREDGAATTPDASADLSPRTGETAETAATVTDAEASGLLAVAHAEP
                     ATQATAADTGPDATAPDAAPTVTDGEADSLSAGTGEAPAESMTTLTVDLAATGTSAGL
                     GDSPASTHTSTRIPDADTTPASPTPAPLTPAASDLSDRTSEPSAAPEPPAAAARDGQP
                     TVDPDAAIEEYLRDPAAADAIARLRAAGPAGLTRIAARFPGPIELGAQGDARSFPPPS
                     AHGPLLRACIELGGVITPHVLDLVDHPRPQIRFYAAFLFQELRDPRSLRPLAAHAFDP
                     DPDVRLIATRVLESYSRAPGFVEATEVVRAELKSRDRERVLLAAEAAGTLRDTHAVPV
                     LIDLLSVRDKQVRETALESLCSITAKHHGYRPAKWKAWYAEHGQQTRLEWVIDALHHR
                     DTAVRRWAADELVRITGHRIAAPPDGEKPGAKYVLAQWQAWFEANGANYS"
BASE COUNT          239 a          829 c         1191 g          383 t
ORIGIN      
        1 gctcgcggtg gcgatcgcac cggcggcggt cgccgggtgg tcggggatcg tggcggccaa
       61 ggtcgggctg ctcgtgatct cgctggcgct gttcatctac gtgtcgtggg tgctgtggcc
      121 gctgcgagtg ttcgccagca cggacgagct gccggcggtg cgccggcgct tctggtgggt
      181 cggcgtcgtg atgatcgcct gcaacgcgct caacatgggg ctcgggatcc tcgcccacgc
      241 gctgcgcggg tgaggcggtc cacgagcctc aggagtagtt cgcgccgttg gcctcgaacc
      301 aggcctgcca ctgcgcgagc acgtacttgg cgcccggctt ctcgccgtcg ggcggcgcgg
      361 cgatccggtg gccggtgatg cgcaccagct cgtcggcggc ccagcggcgc acggcggtgt
      421 cgcggtggtg cagggcgtcg atgacccact cgaggcgcgt ctgctggccg tgctcggcgt
      481 accaggcctt ccacttggcg gggcggtaac cgtggtgctt ggcggtgatc gagcacaggg
      541 actcgagggc ggtctcgcgg acctgcttgt cgcggaccga cagcaggtcg atcaggacgg
      601 gcacggcgtg ggtgtcgcgc agggtgccgg cggcctcggc agcgagcaac acgcgctcgc
      661 ggtcgcggga cttgagctcg gcgcgcacga cctcggtcgc ctcgacgaag ccgggcgcgc
      721 ggctgtacga ctcgagcacg cgcgtggcga tcaggcggac gtcggggtcg gggtcgaagg
      781 cgtgggcggc gagcgggcgc aggctccggg ggtcacgcag ctcctggaac aggaaggcgg
      841 cgtagaagcg gatctgcggg cgcgggtgat cgacgaggtc gaggacgtgc ggcgtgatga
      901 cgccgccgag ctcgatgcag gcgcgcagca gcgggccgtg ggcgctcggc ggcgggaacg
      961 agcgcgcgtc gccctgggcg ccgagctcga tcgggccggg gaagcgcgcg gcgatccggg
     1021 tcaggcccgc ggggcccgcg gcgcgcaacc tggcgatcgc gtcggcggcg gccgggtcgc
     1081 gcaggtactc ctcgatcgcg gcgtcggggt cgaccgtggg ctgaccgtcg cgcgcagccg
     1141 cggccggcgg ctcgggggcg gccgagggct cggatgtccg atcggacagg tcgctcgcgg
     1201 cgggggtgag cggcgcgggg gtggggctcg cgggggtggt gtccgcgtcg gggatccgcg
     1261 tcgaggtgtg cgtgctcgcc ggcgagtcgc cgagacccgc cgaggtgccg gtggccgcga
     1321 ggtcgaccgt cagggtcgtc atgctctcgg ccggcgcctc gcctgtccct gcggacaggc
     1381 tgtcggcctc gccatcggtg accgtcgggg cggcgtcggg tgcggtggcg tccgggccgg
     1441 tgtcggcggc cgtcgcctgg gtcgcgggct cggcgtgggc gaccgcgagc aggccggagg
     1501 cctcggcgtc ggtgaccgtg gcggcggtct cggcggtctc acctgtccgt ggggacaggt
     1561 cggccgaggc gtcgggcgtc gtggccgcgc cgtcctcgcg gaccaggcgc ggcatgttga
     1621 gcgtgatctc gtcggccccc gggacgaccg ggttctggcc ggtcttgctg ccgcgccggc
     1681 gcgcgccggg cagcggcgtc ggggcgatca gcgaggccgg gatcgagagg gtggtcccgg
     1741 actcgctcgc gccgccggtg gacgcagagg cgccgggacg ccgtagatcg ggcatcacga
     1801 gggcggcgtc gccggtgcgc aggcggtcgc cctttttgcc gacggcccgc cgcgcgccgg
     1861 gcggggcgac caggttcggg aggtgcaaga cggcgctcgc gtccgcggga tcggccgggg
     1921 tgagctccga ggaggagctg tcctgggggg caggttcggc ggcggcgtcg tgggtgtccc
     1981 cgggggcagg ttcggcggcg aggggctcgc gggtggcttc gctggcgtcg gcggggtcgg
     2041 gggtgtcgcg ggtgtcctcg gggacaggtt cggcggcggc ggcgtcgcgg gcgtcgcggg
     2101 tgtctgcggg gacaggttcg gcggcgacgg gggcgtccgt ggcggcgtcg gcggagtcgt
     2161 gggtgtctgc ggggacggag ccgtcggatg tggcggagtc ctgcgcggcc gtcgggacag
     2221 cgctctcggc ggcgcgcggc tcgtgggtgt cccgggggac aggttgctcg ctggcggtgg
     2281 acccggcggc ggcctcgtcg gaggttcgac gcggctcgct cgcggcgtcg gacacctgct
     2341 cgcgactggc cggcggggtg ctgtcctcca ggacaggttc gacgcggctc ggcgcggcgt
     2401 cgggtttggc gagccacgcg gtcgcggggg gcagctcgtc gggggcgtcg cggcgggcgc
     2461 ggtgcgcgtc gctcgtggtg tcgccggtgt cgcgacggat cgggttgacg agggcgatca
     2521 cctgacggcg gccgcgtagc ggccggatgc gggtcggctc ctcgggggcg cgggcggcgg
     2581 gcagcgggcg ggtcggctcg ttgtccgtgt tgacgcgcgg cgaggtcgcc tgggccggtc
     2641 gc
//
LOCUS       DASWNJ010000639         2817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_153039, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000639 DASWNJ010000000
VERSION     DASWNJ010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153039"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VGB85_10715"
     CDS             <1..295
                     /locus_tag="VGB85_10715"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEY0134547.1"
                     /translation="EASTMLPGFPGMIAVRLVEGSPAVGRTLGQLSLRLRAGVTVMAI
                     NREPAGTVVPAPSERLQVGDVLALAGTQEATETAREILLRGSGGIELAYLSGG"
     gene            complement(277..1062)
                     /locus_tag="VGB85_10720"
     CDS             complement(277..1062)
                     /locus_tag="VGB85_10720"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0134548.1"
                     /translation="MTAVLRAQGLSHRVPGRTLFTGLSCELRPGRVTAVVGPNGAGKS
                     TLLRLLAGLDAPQAGEVHLGGEDLRTIPPRLRARRLAYLPQRTTLAYDLGVRELVLLG
                     RSPYLPAFGAPAASDLAAVDAALARVDLAGLADRGARGLSGGELQRVMLARMLASEAG
                     VLVLDEPTAALDIGHALAFLGHLRRLAGEGAAVVLALHDLDLARRFTDDAVLLAPDGA
                     LTGPVGHVLSASNLGSAFGVAVRELGNHLVFEPKQPPPQPPLR"
     gene            1198..>2817
                     /locus_tag="VGB85_10725"
     CDS             1198..>2817
                     /locus_tag="VGB85_10725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HEY0134549.1"
                     /translation="MSLVQLRSSSTGQARDLHAFLPMTREEMDARGWDELDVLIINGD
                     AYVDHPAFGGALIGRFLQGQGLRVGMIAQPRWDDPSDVQRLGRPRLFVGITAGNLDSM
                     LNKLTAQKKIRSEDQYSPDGAVDRRPDRASIAYSGLARRAFPGLPVVLGGIEASLRRI
                     AHYDYWSDQVRRSVLIDSKADLLVFGMGERPIMEIAARLKAGETIDQLTDVRGTGRML
                     NPGQWEALPRSRFVRDGKTLWLPSYEEVKADKVAFMEMSRAFQHETNPGNGRPLLQPH
                     GERAVYLNPPAQPLKTEDMDRLYDLPFVRRAHWSYGSQKIPAFETVKHSIVTMRGCFG
                     GCSFCSITEHEGRIIQSRSAESVLREVRSLRRMDDFRGVISDVGGPTANMYQMKCKSE
                     AIESSCRRLSCVHPGVCENLQTDHGPLIDLLRRVRNEDGIKRVFIASGVRYDLAERSP
                     AFIKELARHHTGGQLSVAPEHVKDDVLAKMKKPGVASYERFAEQFNCASQEAGKDQHL
                     VPYYISGHPGSTLQDMIDLALYLKARNMRPRQ"
BASE COUNT          421 a          933 c         1106 g          357 t
ORIGIN      
        1 cgaggcctcg acgatgttgc cgggcttccc gggcatgatc gcggtgcgcc tggtcgaggg
       61 cagcccggcc gtcggtcgca cgctcggcca gctcagcctg cgcctgcgcg cgggcgtgac
      121 ggtgatggcg atcaaccgcg agccggccgg cacggtggtt ccggcgccga gcgagcgcct
      181 gcaggtcggc gatgtgctgg ccctcgccgg cacgcaggag gcgaccgaga cggcgcgcga
      241 gatcctgctg cgggggtccg gcgggatcga gctggcctac ctcagcggcg gctgaggcgg
      301 cggctgcttc ggctcgaata cgaggtgatt gccgagctcg cgcacggcga cgccgaaggc
      361 cgagccgagg ttgctggccg agaggacatg tcccacggga ccggtcaagg cgccgtcggg
      421 cgcgagcagc acggcgtcgt cggtgaagcg gcgcgcgagg tcgaggtcgt gcagcgcgag
      481 cacgacggcg gcgccctcgc cggcgaggcg gcgcaggtgg ccgaggaagg ccagcgcgtg
      541 gccgatgtcg agggcggcgg tcggctcgtc gagcacgagc acgccagcct cgctggcgag
      601 catgcgcgcg agcatgacgc gctgcagctc gccgccgctg agcccgcgcg cgccgcggtc
      661 ggcgaggccc gcgaggtcga cccgggcgag cgcggcgtcg acggcggcga ggtcgctggc
      721 cgccggggcg ccaaaagccg gcaggtaggg cgagcgcccg agcagcacga gctcgcggac
      781 gccgaggtcg taggcgaggg tggtgcgctg cggcaggtag gccaggcggc gcgcccgcag
      841 gcgcggtggg atcgtgcgca ggtcctcgcc gccgaggtgg acctcgccgg cctgcggcgc
      901 gtcgaggccg gcgagcaggc gcagcagggt cgacttgcct gccccgttgg ggccgacgac
      961 ggcggtgacg cgaccgggcc gcagctcgca ggagaggccg gtgaagaggg tgcgccccgg
     1021 gacccggtgg ctcaggccct gcgcgcggag gacggcggtc acgcgcgcga gggtgccagg
     1081 ggccggggcg caggagcaag cgtgtcggga ggcgccggcg ccggcgaatt ggtccgcgtg
     1141 gcctcgcgca cgcgcgcggg ggtgtcggcg ggggtcggat ggacttacgg tgcccgcgtg
     1201 tcgctcgtcc agctgcgatc gtcgagtacg ggccaggccc gcgacctgca tgcgttcctc
     1261 ccgatgacgc gggaggagat ggacgcgcgc ggctgggacg agctcgacgt cctgatcatc
     1321 aacggcgacg cgtacgtcga tcacccggcg ttcggcgggg cgctgatcgg gcggtttttg
     1381 caggggcaag ggctgcgcgt cggcatgatc gcgcagccgc gctgggacga cccgtccgac
     1441 gtgcagcgcc tcggccgacc gcggctgttc gtcgggatca cggcgggcaa cctcgactcg
     1501 atgctcaaca agctgacggc gcagaagaag atccgcagcg aggatcaata ctcgccggac
     1561 ggcgccgtcg accgccgccc cgaccgcgcc tcgatcgcgt actcgggcct cgcgcgtcgg
     1621 gcgttcccgg ggctgccggt ggtgctcggc gggatcgagg cgagcctgcg gaggatcgcc
     1681 cactacgact actggagcga ccaggtccgt cgcagcgtgt tgatcgacag caaggccgac
     1741 ctgctggtgt tcggcatggg cgagcggccg atcatggaga tcgcggcgcg gctcaaggcc
     1801 ggcgagacga tcgaccagct gaccgacgtc cgcggcaccg ggcgcatgct caacccgggc
     1861 cagtgggagg cgctgcccag gagccgcttc gtgcgcgacg gcaagaccct gtggctgccg
     1921 agctacgagg aggtcaaggc cgacaaggtc gcgttcatgg agatgtcgcg cgccttccag
     1981 cacgagacca acccgggcaa cgggcgcccg ctgctgcagc cgcacggcga gcgcgcggtg
     2041 tacttgaacc cgccggcgca gccgctcaag accgaggaca tggaccggct gtacgacctg
     2101 ccgttcgtgc gccgcgccca ctggtcgtac gggagccaga agatcccggc gttcgagacg
     2161 gtcaagcact cgatcgtgac gatgcgcggc tgcttcggcg gctgcagctt ctgctcgatc
     2221 accgagcacg agggccggat cattcagtcg cgctcggccg agagcgtgct gcgcgaggtc
     2281 cggtcgctgc ggcgcatgga cgactttcgc ggggtgatca gcgacgtcgg cgggccgacg
     2341 gcgaacatgt accagatgaa gtgcaagagc gaggcgatcg agtcgtcgtg ccggcgcctg
     2401 tcctgcgtgc atccgggcgt gtgcgagaac ctgcagaccg accacgggcc gctgatcgac
     2461 ctgctgaggc gcgtacgcaa cgaggacggg atcaagcggg tgttcatcgc ctcgggggtc
     2521 cgctacgacc tcgcggagcg ctcgccggcg ttcatcaagg agctcgcgcg gcatcacacc
     2581 ggcgggcaat tgtcggtggc gccggagcac gtcaaggacg acgtgctcgc caagatgaag
     2641 aagccgggcg tcgccagcta cgagcgcttc gccgagcagt tcaactgcgc gagccaggag
     2701 gccggcaagg accagcacct ggtgccgtat tacatctccg ggcacccggg cagcaccctc
     2761 caggacatga tcgacctcgc gctgtacctc aaggcccgca acatgcggcc gcgccag
//
LOCUS       DASWNJ010000640         7488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_470714, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000640 DASWNJ010000000
VERSION     DASWNJ010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7488
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470714"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2046
                     /gene="mrdA"
                     /locus_tag="VGB85_10730"
     CDS             <1..2046
                     /gene="mrdA"
                     /locus_tag="VGB85_10730"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY0134550.1"
                     /translation="EGEHYVRRAERNFISTIEVEAPRGRIFDAERRPLASNRPAYTLF
                     VTPRPRVVLETEDPAKKSQTGSRVPLTEAQIDSVADLIDFVDAEDRAEFEAKIRDRRD
                     GENGFYPVAVRQNLSWSEFARIQTRKSALGDWVEIRESARRYYPAGELTSFVTGYVGE
                     ITPDGLARSPGSYRPGDRVGKTGIERQWENYLRGRPGKKSHVVNVRSRAVKSPPPEAL
                     AALPPDETPIPGQDIYLTLDLDLQQVAAEAFADKPAGALVAMDPRTGKILAMLSVPAI
                     DPNRWQQPIGREQYTAWAKSPLKPFIDRTVQEHYFPGSTYKVVSALASLNDPTFDPEE
                     EITCDGYVNYGGRKFKCTHKHGPVNLEQAIVQSCNVYFYTLAIDKNLSLERMEQFARR
                     LGLGEKTGLGLNSEAKGRIPTEAFEAREGTYQRGVRLNSAIGQGNVTATVLQVAVLYS
                     AIANGGSVMTPYLVDQIRTADGKLVFQSVPKKRPDLAPVLGPDRERIHQGLVGVVNNE
                     LGTAYSERLDQIVVAGKTGTAQVGASDRKGPRAATLEGFDVTQDHAWFAGYAPAENPQ
                     IVVVAFVEHGGVGADAAAPIVMKVIEYYLGKAQAGAEGPQPRRTGIGPPLPGQRAREV
                     AAREDSTTPSPDRSVALTGARLPGERPLVGDLPSEPAVPGGPKPTKPPPAPARKEAP"
     gene            2043..3158
                     /gene="rodA"
                     /locus_tag="VGB85_10735"
     CDS             2043..3158
                     /gene="rodA"
                     /locus_tag="VGB85_10735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEY0134551.1"
                     /translation="MSTLGRSRSLPHRIFHSIDWVIVTLVAGIVTLALINLNSAGSGD
                     WTGKIQTQIRWIGLGTIATIITASLDYRVLHRLAYAAYFFGLALLGAVPVFGVMRNEA
                     RRWLEFGEYQFQPSELMKILLIMALARYISDLPPKEHKRLRHLVIPFAMFLVPVVLIV
                     NQPDLSTGIISTLVAMTILALTELTLKALLTLLSAGVGLFMLGWGFFMHKYQRSRIDV
                     WLNPEAYADNEGYQTIQAMIGVGNGGFFGRGVGKGTQNVLDFLPYGDTDFPFAVYAEE
                     WGFVGAAMVLAMFLCLVLWSINLASQARDRFGALLCIGVASVFFWHVVINIGMVLQLL
                     PVTGITIPLFSLGGSNALAMMLGIGILMSVSRSRIRP"
     gene            3404..3724
                     /locus_tag="VGB85_10740"
     CDS             3404..3724
                     /locus_tag="VGB85_10740"
                     /inference="COORDINATES: protein motif:HMM:NF024232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HEY0134552.1"
                     /translation="MLNNPIGRLRLIGLLEGSSFLILLLIAMPLKYAAGLPLAVKYVG
                     WAHGALFLLFILALGQAAAETDWGFKKIAGAFIASVVPLGTFVLDRQWKQDEAALLAA
                     PHAS"
     gene            4004..5308
                     /locus_tag="VGB85_10745"
     CDS             4004..5308
                     /locus_tag="VGB85_10745"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0134553.1"
                     /translation="MVTRLLRLSPLLLLAAGCLRGNGGLGDDSSTGLPADTSSTGEPD
                     STSSSSTSTTDASTTAASTGSSTGQPPDTTGTLDTTGEPPLCGNGELDDGELCDDGPD
                     NSDKAACKLDCTPQTCGDGELGPGELCDDGNLDDTDACTAACVPATCGDGLVHTGVEA
                     CDDGDDDDLDACTVACVHNVCGDGRLHTGVEVCDDGEATATCDADCTPPDCGDGLVNA
                     PAGELCDDGNAIDSDACTTQCQPAACGDGFVQAGETCDDGGLIPGDGCDPFCKKEAIK
                     CQNQATVVSVAPGNRAVLCQRPEICEQDFALLCPKDWHLCSADEFNARNTAWNYTPTK
                     LTLGSIRCRQGTGAGQYGFKSTMSLDHVDNCLYSSSHPQCAGNFGCDEKNNYALCCAP
                     LAKCGDGVLDPEEQCDDGNLSDSDQCLRNCLKKHAPGVQGCG"
     gene            complement(5312..5800)
                     /locus_tag="VGB85_10750"
     CDS             complement(5312..5800)
                     /locus_tag="VGB85_10750"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEY0134554.1"
                     /translation="MQRLRTWIRGLLTLHGTPRGIAGGFALGVGLSLIPVPFLGMVLA
                     LAAAPVVRANVPATYLGTAVINPFTGVVFYFGELWLGTLLLGRPLPSWATLSMLDAGG
                     WWALFKELLGPFLLGAAAAIPIVAGLSYVLVFWVVQRWQRRNATPVTPVTPVTPDAPV
                     GE"
     gene            complement(5800..6171)
                     /locus_tag="VGB85_10755"
     CDS             complement(5800..6171)
                     /locus_tag="VGB85_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134555.1"
                     /translation="MRKTIAMFVVLGLFGCGDSPEATRARDEAGKAADQAGKAARAAG
                     DAAVEAAKTGAAEAEVAADKMADKAAELGAAGKVHAERAAVEGKAALEVAGERSKEAM
                     ERSKAEMNEAIEKARSKAKED"
     gene            6313..6918
                     /locus_tag="VGB85_10760"
     CDS             6313..6918
                     /locus_tag="VGB85_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEY0134556.1"
                     /translation="MGRPLASADALATPERLLDAAEQSFARAGFGAARLEDIAAAAGI
                     RRPSLLYHYPSKGSLYAAVVRRTFTRLGAALMQAMARPVDFVARLEGVVTTYAGFLAE
                     HPAHAQILVRELIDPDGPGREILLGEVVPLLNVLERFVGVEGHGVVRPDLPVRAAILQ
                     CASGIVLRSAAGALQGPLWGPVDHAGVLARVLFFGPSRSEV"
     gene            6920..>7488
                     /locus_tag="VGB85_10765"
     CDS             6920..>7488
                     /locus_tag="VGB85_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134557.1"
                     /translation="MSSLIERANEYSPSHMLQKVLRTGNVRVALNLLGPALRGLVMHS
                     GKQPSSDMQANHTAHFDWTYQRDQPELRKLYEAAKVSQWNATTDLDWSIDVDPYNERV
                     DLFPEVLSPLPDIPQYRGLSTREKQTHRHGLVSWMLSQFLHGEQGALFAACQVTEAIT
                     WLDGARAGRGPRGRRAGGPAGLGAPGSRAT"
BASE COUNT         1128 a         2951 c         2329 g         1080 t
ORIGIN      
        1 gagggtgagc attacgtgcg tcgcgccgag cgcaacttca tcagcacgat cgaggtcgag
       61 gccccgcgcg gccgcatctt cgacgccgag cgccgcccgc tcgccagcaa ccggcccgcg
      121 tacaccctct tcgtcacccc gcgcccgcgc gtcgtgctcg agaccgagga ccccgccaag
      181 aagtcgcaga ccggctcgcg cgtccccctg accgaggcgc agatcgacag cgtcgccgac
      241 ctgatcgact tcgtcgacgc cgaggaccgc gccgagttcg aggccaagat ccgcgatcgt
      301 cgtgacggcg agaacggctt ttatccggtc gccgtgcgcc agaacctgag ctggtccgag
      361 ttcgccagga tccagacccg caagtcggcg ctcggcgact gggtcgagat ccgcgagtcg
      421 gcgcggcgct actatcctgc cggcgagctg acctccttcg tcaccggcta cgtcggcgag
      481 atcacgcctg acggcctcgc ccgatcgccc ggcagctacc gccccggcga ccgcgtcggc
      541 aagaccggca tcgagcgcca gtgggagaac tacctccgcg gccggcccgg caagaagtcg
      601 cacgtcgtca acgtgcgcag ccgggccgtc aagtcgccgc cgcccgaggc cctcgcggcg
      661 cttccacccg acgagacccc gatccccggt caggacatct acctcaccct cgatctcgat
      721 ctacaacagg tcgccgccga ggccttcgcc gacaagccgg ccggcgcgct ggtggcgatg
      781 gacccacgca ccggcaagat cctggcgatg ctctcggtcc ccgcgatcga ccccaaccgc
      841 tggcagcagc cgatcggccg cgagcagtac accgcgtggg ccaagagccc gctcaagccg
      901 ttcatcgacc gcacggtgca ggagcactac ttccccggct cgacctacaa ggtcgtctcg
      961 gcgctcgcct cgctcaacga cccgaccttc gaccccgagg aggagatcac ctgcgacggc
     1021 tacgtcaact acggcggccg caagttcaag tgcacgcaca agcacggccc cgtcaacctc
     1081 gagcaggcga tcgtgcagtc gtgcaacgtg tacttctaca cgctggcgat cgacaagaac
     1141 ctcagcctcg agcgcatgga gcagttcgcg cggcgcctcg gtctcggcga gaagaccggc
     1201 ctcggcctca actcggaggc caagggccgg atcccgaccg aggccttcga ggcgcgtgag
     1261 ggcacctacc agcgcggcgt ccggctcaac agcgcgatcg gccagggcaa cgtcaccgcg
     1321 accgtcttgc aggtcgccgt gctctactcg gcgatcgcca acggcggcag cgtcatgacg
     1381 ccttacctcg tcgaccagat ccgcaccgcc gacggcaagc tcgtgttcca gagcgtgccc
     1441 aagaagcgcc ccgacctcgc gccggtcctc ggccccgacc gcgagcggat ccaccagggc
     1501 ctcgtcggcg tcgtcaacaa cgagctcggc accgcctaca gcgagcgcct cgaccagatc
     1561 gtcgtcgccg gcaagaccgg caccgcgcag gtcggcgcca gcgaccgcaa gggcccgcgc
     1621 gccgccaccc tcgagggctt cgacgtcacc caggaccacg cctggttcgc cggctacgcc
     1681 cccgccgaga acccgcagat cgtcgtcgtc gccttcgtcg agcacggcgg cgtcggtgcc
     1741 gatgcggccg cgccgatcgt catgaaggtg atcgagtact acctcggcaa ggcccaggcc
     1801 ggcgccgagg gcccgcagcc gcgacgcacc ggcatcggcc cgcctttgcc cggccagcgc
     1861 gcccgcgagg tcgccgcgcg cgaggacagc accaccccga gccccgaccg ctccgtcgcg
     1921 ctgaccggcg cgcgcctgcc tggcgagcgg ccgctcgtcg gtgacctgcc gagcgagccc
     1981 gccgtccccg gcggccccaa gccgaccaag cccccgcccg cgccggcgcg caaggaggcc
     2041 ccatgagcac gctcggccgc tcgcgctcgt tgccccaccg gatctttcac tcgatcgact
     2101 gggtgatcgt caccctcgtc gccggcatcg tcaccctcgc gctgatcaac ctcaacagcg
     2161 ccggctcggg cgactggacc ggaaagatcc agacccagat ccggtggatc ggcctgggca
     2221 cgatcgccac gatcatcacg gcctcgctcg actatcgcgt gctgcaccgg ctcgcctacg
     2281 ccgcctactt cttcggcctc gcgctgctcg gcgccgtgcc ggtgttcggc gtcatgcgca
     2341 acgaggcccg ccgctggctc gagttcggcg agtatcagtt tcagccctcc gagctgatga
     2401 agatcctgct gatcatggcg ctggcccgct acatcagcga cctcccgccc aaggagcaca
     2461 agcgcctgcg ccacctggtg atccccttcg ccatgttcct ggtcccggtc gtgctgatcg
     2521 tcaaccagcc cgacctgagc acggggatca tctcgacgct cgtggcgatg acgatcctcg
     2581 cgctgaccga gctgacgctc aaggccctgc tgaccctgct gagcgccggc gtcggcctgt
     2641 tcatgctcgg ctggggcttc ttcatgcaca agtaccagcg ctcgcgcatc gacgtgtggc
     2701 tcaaccccga ggcctacgcc gacaacgagg gctaccagac gatccaggcg atgatcggcg
     2761 tcggcaacgg cggcttcttc ggccgcggcg tcggcaaggg cacccagaac gtcctcgact
     2821 tcctgcctta cggcgacacc gacttcccgt tcgccgtcta cgccgaggag tggggcttcg
     2881 tcggcgccgc gatggtcctg gcgatgttcc tgtgcctggt cctgtggtcg atcaacctcg
     2941 cctcgcaggc ccgcgaccgc ttcggcgcgc tgctgtgtat cggcgtcgcc tcggtgttct
     3001 tctggcacgt cgtgatcaac atcggcatgg tcctccagct gctcccggtc accggcatca
     3061 cgatcccgct gtttagcctc ggtggctcca acgccctggc gatgatgctc ggcatcggga
     3121 tcctgatgag cgtcagccgc tcgcggatcc gaccctgacc cccgcgccgg cacgcgccac
     3181 gagaccagga cccgctcatg gtccccggca tgatcttcgc ggtccttcgg cgggtgcctc
     3241 gcggccggct gccgcgccct cggcatgctg atcctcgcgg tgctcggcgg cctgctggcg
     3301 ctcgacgctc tcgcggccgc cgtccacaac gcacgccggc tgtgacccgt gacccgctgc
     3361 cgcggcgctt ggcgggccgc gcggcgcttg ctagggtcgc cccgtgctca acaaccccat
     3421 cggccgcctg cgcctgatcg gcctgctcga gggcagctcc ttcctcatcc tgctcctgat
     3481 cgccatgccg ctcaagtacg ccgccggcct gccgctcgca gtcaagtacg tcggctgggc
     3541 ccacggcgcc ctgttcctgc tgttcatcct cgccctcggc caggccgccg ccgagaccga
     3601 ctggggtttt aagaagatcg ccggagcctt catcgcctcg gtcgtccccc tcggcacctt
     3661 cgtgctcgac cggcaatgga agcaggacga agcagccctg ctcgcggctc ctcacgcgag
     3721 ttagccgcca ccgcacgctc gccgagcgct ggccgtccgg atcctccatc cgggcggcct
     3781 ttgtgcgtcc ggtccactgc ccgaactgct ccgtcgcggc gccgggccta tccgagcgcc
     3841 cgcgccgtca cgcgcggctt cgctcagtgc gcgcgcatgc gagcatgcgc gcacttggtc
     3901 tcttggagag caagcgtcca cgaattatca tgcaagactg cgttttgaag cctcgcggtg
     3961 gcgctgcccc gcggccgccg ttcgtcgtct cttttggtcc gtcatggtca cccgtctgct
     4021 gcgcctgtct cccctgttgc tgctcgcggc gggctgcctg cgtggcaacg gcggcctcgg
     4081 tgacgactcg agcaccggcc tgcccgcgga cacctcctcc acgggcgagc ccgactccac
     4141 ctcgtcgagc agcaccagca ccaccgacgc cagcaccacc gccgccagca ccggcagctc
     4201 gaccggccag ccccccgaca ccaccggcac gctcgacacc accggcgagc cgccgctgtg
     4261 cggcaacggt gagctcgatg atggcgagct gtgtgacgac ggccccgaca acagcgacaa
     4321 ggccgcctgc aagctcgact gcaccccgca gacctgcggc gacggcgagc tcggcccggg
     4381 cgagctgtgc gacgacggca acctcgacga caccgacgcc tgcaccgcgg cctgcgtccc
     4441 tgccacttgc ggcgacggcc tcgtgcacac cggcgtcgag gcctgcgacg acggcgacga
     4501 cgacgacctc gacgcctgca ccgtcgcgtg tgtgcacaac gtctgcggcg acggccggct
     4561 gcacaccggc gtcgaggtct gcgacgacgg cgaagcgacc gccacctgcg acgcggactg
     4621 caccccgccc gactgcggcg acggcctcgt caacgcgccc gcgggcgagc tgtgcgacga
     4681 cggcaacgcg atcgacagcg acgcctgcac cacgcagtgc cagcccgcgg cctgcggtga
     4741 cggcttcgta caggccggcg agacctgcga cgacggcggc ctgatccccg gcgacggctg
     4801 cgatcccttt tgcaagaaag aggcgatcaa gtgccagaac caggccaccg tcgtcagcgt
     4861 cgcgccgggc aaccgcgccg tgctgtgcca gcggcccgag atctgcgagc aggacttcgc
     4921 gctgctgtgc cccaaggact ggcacctgtg cagcgccgac gagttcaacg cgcgcaacac
     4981 ggcctggaac tacaccccga ccaagctgac cctcggctcg atccgctgcc gccagggcac
     5041 cggcgccggc cagtacggct tcaagtcgac catgtccctc gatcacgtcg acaactgcct
     5101 gtacagcagc tcccacccgc agtgcgccgg caacttcggc tgcgacgaga agaacaacta
     5161 cgccctgtgc tgcgccccgc tggcgaagtg cggcgacggt gtgctcgacc ccgaggagca
     5221 gtgcgacgac ggcaacctct cggacagcga ccagtgcctg cgcaactgcc tcaagaagca
     5281 cgcccccggc gtacagggct gcggctgacg cctactcgcc cacgggtgca tccggcgtca
     5341 ccggcgtcac cggcgtcacc ggcgttgcat tgcgccgctg ccaccgctgc acgacccaga
     5401 acaccagcac gtagctgagc cccgccacga tcgggatcgc agccgcggcc ccgagcagga
     5461 acggcccgag cagctccttg aacagcgccc accagccgcc cgcgtcgagc atcgacagcg
     5521 tcgcccagct cggcagcggg cgcccgagca gcagcgtccc gagccacagc tcgccgaagt
     5581 agaacaccac ccccgtgaac gggttgatga ccgccgtgcc gaggtaggtc gccggcacgt
     5641 tggcgcggac gaccggcgcg gccgccagcg ccagcaccat gccgaggaac ggcacgggga
     5701 tcagcgacag gccgaccccg agcgcgaagc ccccggcgat cccacgcggc gtcccgtgca
     5761 acgtcaggag cccgcggatc catgtccgca ggcgctgcat caatcctcct tcgccttgct
     5821 ccgggccttc tcgatcgcct cgttcatctc cgccttgctg cgctccatcg cctccttgct
     5881 gcgctcgccc gcaacctcga gcgccgcctt gccctcgacc gccgcacgct cggcgtgcac
     5941 cttgccggcc gcgcccagct ccgccgcctt gtccgccatc ttgtcggccg cgacctccgc
     6001 ctccgcggcc ccggtcttcg ccgcctcgac cgccgcgtcg cccgcggccc tcgccgcctt
     6061 gcccgcctgg tcggccgcct tcccggcctc gtcgcgggcc cgcgtcgcct ccggcgagtc
     6121 accgcagccg aacaacccga gaaccacgaa catcgcgatg gtcttgcgca tggccgcaag
     6181 cgtggggccc gagcgccgcg atcgcaagcc accgccccgc gcacatccgc gccacccccc
     6241 accaaaccct cgtattcacg ccccaaacac cccacttgac aaatctactc acgagtagat
     6301 atcgtcctgg acatgggtcg cccgctcgcc agcgccgatg cactcgccac gcccgagcgc
     6361 ctgctcgacg cggccgagca gagcttcgcg cgcgccggct tcggcgcggc gcggctcgag
     6421 gacatcgccg cggccgcggg catccgccgg ccctcgctgc tctaccacta cccgtccaag
     6481 ggcagtctct acgccgcggt cgtgcgtcgc acctttacgc gcctcggcgc cgccctgatg
     6541 caggcgatgg cgcgcccggt cgacttcgtc gcgcgcctcg agggtgtcgt caccacctac
     6601 gccggcttcc tcgccgagca ccccgcgcac gcgcagatcc tcgtgcgcga gctgatcgac
     6661 cccgacggtc ccggccgcga gatcctgctc ggcgaggtcg tccctctatt gaatgtgctc
     6721 gagcgcttcg tcggcgtcga gggtcacggc gtggtccgcc ccgacctgcc ggtgcgcgcc
     6781 gcgatcttgc aatgtgccag cggcatcgtc ctgcggtccg ccgccggcgc gctgcaggga
     6841 cccctgtggg gtcccgtcga tcacgcgggc gtgctcgccc gggtgttgtt ctttggtccg
     6901 tcacgatcgg aggtgtgagg tgtccagtct catcgagcgc gcgaacgaat acagcccgtc
     6961 ccacatgctg caaaaggtgc tgcgcaccgg caacgtccgc gtcgcactca acctgctcgg
     7021 cccggccctg cgcggcctcg tgatgcactc cggcaagcag ccgagcagcg acatgcaggc
     7081 caaccacacc gcccacttcg actggaccta ccagcgcgac cagcccgagc tgcgcaagct
     7141 ctacgaggcc gccaaggtct cgcagtggaa cgcgaccacc gacctcgact ggtcgatcga
     7201 cgtcgaccct tacaacgaga gggtcgacct gttccccgag gtcctcagcc cgctcccgga
     7261 catcccgcag tatcgcggcc tgtcaacgcg cgagaagcag acccaccgcc acggcctcgt
     7321 cagctggatg ctgtcgcagt tcctgcacgg cgagcagggc gcgctgtttg ccgcctgcca
     7381 ggtcaccgag gcgatcacct ggctcgacgg cgcgcgagcc gggcgagggc cccgaggacg
     7441 gcgagcgggt ggtcctgcgg gtctcggagc gccgggcagt cgagcgac
//
LOCUS       DASWNJ010000641         5170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_397339, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000641 DASWNJ010000000
VERSION     DASWNJ010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5170
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397339"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1147
                     /locus_tag="VGB85_10770"
     CDS             185..1147
                     /locus_tag="VGB85_10770"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY0134558.1"
                     /translation="MSWSSCPDPTPGDRTSVEAIELLIVPRAVDLGEMTVRRALPSIK
                     RQMVGPFIFFDQMGPAEFLGGQGIDVRPHPHINLATVTYLFEGQILHRDSLGTEKTIE
                     PGAVNWMRAGRGIVHSERTSPERKRDGQRLFGIQTWMALPVDQEESDPAFVHHPATEL
                     PMVDADGVRARLIAGAAFGLRSPLETPSETLYADVQLAAGAILEIDAGYDERAIYTVG
                     GDLEVAGDRFEPGQLLVLRSGEKIVVRARGEARFMICGGAPMEGPRYIWWNFVSSRLE
                     RIEQAREDWAQGRFDTVPGDTEEFIPLPQTQDKPRRATGGVFYP"
     gene            complement(1138..2238)
                     /locus_tag="VGB85_10775"
     CDS             complement(1138..2238)
                     /locus_tag="VGB85_10775"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0134559.1"
                     /translation="MATEDPRIEIDGVVFVWDRARDLFLINGMPAVGLLVESTVAQLL
                     VGVERMVGLERFNLALQAAGRDSVAGEWEGFISKMPSVREGLEMIGQVAPLGGLGRWQ
                     LLELDVPGKIARFRVTAGWEPMYQQAAGVAFGSSFLAGKFAGYCTRAFGTYCWAEQTA
                     SVVHGDAYDEFVVRPSDSRLEARLEDLQSEGKATASDLASALKRLQHEAQERSRAEAG
                     LREQLAVVERQAEAIRTMSSPILRVWSGVLAMPVVGRVDATRAARMMEDLLSAVTSTR
                     ARWTILDLTGVDTIDAAALHEIMGLVRAVALLGARCLLSGMSPTVATTAVELGVDLAG
                     LASHATLESALQVAMQAMQKTQMMQKTRRPQG"
     gene            complement(2320..4146)
                     /locus_tag="VGB85_10780"
     CDS             complement(2320..4146)
                     /locus_tag="VGB85_10780"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HEY0134560.1"
                     /translation="MRARTIVFLGGLTMSCGEPGEGSGSSPGASTGTSGGSSAGVDPT
                     GTGEATGGAPTATGSDTGEPTGVATHTIGGSVRGLQGAGLELALAGEVLAIAGDGPFD
                     FVTPAGEGASYSVTVATQPGAPDQTCMIAHGEGLVAGADVGDIVVTCVTPIRHVVVLG
                     IDGLGGAYVPEVATPVLDGMMQAGVSTLAMQNTLPTMSAPNWMSMIVGSTADQHGVDS
                     NEWEPGDSQPTPTIFAVLRAQRPTARIGVFHDWDGFGALVEPGVADVITSPGDEVETV
                     SAALAWMQGNAPDLLFIHLDLVDHAGHFNGWGSDAYVEAAGSADALVGEVLAGIDAAG
                     MRPYTAVLVSADHGGDGLSHGADTSLERPIPLIVTGPQVPAGVQLQRETRIFDIAATV
                     AALHGLAAPASWLGSPVHEALAFVDPPDEPARPLELLPVSDYVWVYDDSGSGALTDGS
                     IWRPVVPAGFVSVGDVAQASHDAPGFATLVIRDDPGAVREPVGYEQIWTDEGSLGDHD
                     VTIWNPIPPAHFVCLGSVAVAAYDTPPATGLIRCVHRRYLVRGAMTLTWTDTGSFAFQ
                     DAGLWTCTDGVEGGQAARSFITRRHHSDPGLPRCWSLVTGRG"
     gene            4278..>5170
                     /locus_tag="VGB85_10785"
     CDS             4278..>5170
                     /locus_tag="VGB85_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134561.1"
                     /translation="MSTRLRLSFALCAPLLASCGGEPSTTDTGDASTSASADTSTGDI
                     PVDPTGAPTTTGASTTDTPDTTDTSGGEVHVVPQFGGTFTVIGTAADGLNVVRDLEFH
                     PGAPDQLWTYNMLSHGVVIFFAPGSAEQTSEVRIDAYGQHFMAYVSSAAFGVDGKFAT
                     CQESRNEWNVGPQKPDDFMGPALWSADLDIFAKVHQEVPPNPLEGSHLDMLHQSPLCM
                     GIAHDTANVYWIFDGKNSDIVRYDFQTDHGPGGGDHSDGVITRYYDAEVTRIANIPGH
                     LEIDHATGMLYVADTGGGRIMR"
BASE COUNT          775 a         1934 c         1738 g          723 t
ORIGIN      
        1 tcctgctacg tcggcgagga cgcgtgcggg ccgaagtcgg attgcgacaa cctcggcttt
       61 gcacccgacg ccgagggcaa ggtcgtcggc gtctgcagcc agccgccgac ctgaccggac
      121 ccgaagggcg cgtccgggca aaatccgtgg cggccgtcgg gttcgctctg gtacatgacc
      181 tgtcatgagc tggagctcct gccccgatcc cacccctggc gaccgcacga gcgtggaggc
      241 gatcgagctg ttgatcgtgc cgcgtgcggt cgacctcggc gagatgaccg tgcgccgcgc
      301 gctgccgtcg atcaagcggc agatggtcgg gcccttcatc ttcttcgatc agatgggccc
      361 ggccgagttc ctcggcggac agggcatcga cgtgcggccg cacccgcaca tcaacctcgc
      421 caccgtcacc tacctgttcg agggtcagat cctgcaccgc gacagcctcg gcaccgagaa
      481 gacgatcgag cccggcgccg tcaactggat gcgcgccggt cgtggcatcg tgcacagcga
      541 gcgcaccagc cccgagcgca agcgcgacgg ccagcgcctg ttcgggatcc agacctggat
      601 ggcgctgccg gtcgatcagg aggagagcga ccccgccttc gtgcatcacc ccgcgaccga
      661 gctgccgatg gtcgacgccg acggcgtgcg ggcgcgcctg atcgccggcg ccgcgttcgg
      721 cctgcgctcg ccgctcgaga ccccctcgga gacgctctac gccgatgtcc agctcgccgc
      781 cggtgcgatc ctcgagatcg atgcgggcta cgacgagcgg gcgatctaca ccgtcggcgg
      841 cgacctcgag gtcgcgggcg accgcttcga gcccggccag ctgctggtgc tgcggtccgg
      901 cgagaagatc gtcgtgcgcg cgcgcggcga ggcccgcttc atgatctgcg gcggcgcgcc
      961 gatggagggg ccgcgctaca tctggtggaa cttcgtctcg tcgcggctcg agcggatcga
     1021 gcaggcgcgc gaggactggg cgcagggtcg cttcgacacc gtgcccggcg acacggagga
     1081 gttcatcccg ctgccgcaga cgcaggacaa gccgcgccgc gcgaccggcg gggtgttcta
     1141 cccctgaggc cggcgcgtct tctgcatcat ctgcgtcttc tgcatcgcct gcatggccac
     1201 ctgcaacgcc gactcgagcg tcgcgtggct ggcgagcccg gcgaggtcga caccgagctc
     1261 gacggcggtc gtcgccacgg tcggtgacat gccggacagc aggcagcgcg cgccgagcaa
     1321 ggcgaccgcg cgcacgaggc ccatgatctc gtgcagcgcc gcggcgtcga tcgtgtcgac
     1381 gccggtgagg tcgaggatcg tccagcgcgc gcgcgtcgag gtcaccgccg acagcaggtc
     1441 ctccatcatc cgcgccgccc gggtggcgtc gacgcggccg accaccggca tcgccagcac
     1501 gcccgaccac acgcgcagga tcggcgagct catcgtccgg atcgcctcgg cctggcgctc
     1561 gaccaccgcg agctgctcgc gcaggcccgc ctccgcccgc gaccgctcct gcgcctcgtg
     1621 ttgcagccgc ttgagcgcgc tcgccaggtc gctcgccgtc gccttgccct cgctctgcag
     1681 gtcctcgagg cgtgcctcga gccggctgtc cgagggccgc acgacgaact cgtcatacgc
     1741 gtcgccgtgc acgaccgacg cggtctgctc ggcccagcag taggtcccga acgcgcgcgt
     1801 gcagtagccg gcgaacttgc cggcgaggaa gctcgacccg aaggcgacgc cggccgcttg
     1861 ctgatacatc ggctcccagc cggcggtgac gcggaagcgg gcgatcttcc ccgggacatc
     1921 gagctcgagc agctgccagc gcccgagccc gccgagcggc gcgacctgcc cgatcatctc
     1981 gaggccctcg cgtacgctcg gcatcttcga gataaagccc tcccactcgc ccgcgacgct
     2041 gtcgcggccg gccgcctgca acgcgaggtt gaagcgctcg aggccgacca tgcgctcgac
     2101 gccgacgagc agctgggcca ccgtgctctc gaccagcagg ccgaccgccg gcatcccgtt
     2161 gatcaggaac aggtcgcgcg cgcggtccca gacgaatacg acgccgtcga tctcgatgcg
     2221 ggggtcttcg gtggccatcg cgaccgacaa gctagcaaaa ctccgtatga acgcggtgta
     2281 tcgcgtctgc actcgacccg tcgcatcgcg tgtgggtgct tacccccggc cggtcacgag
     2341 cgaccagcag cgcggcaacc cggggtcgct gtgatgtcgt cgcgtgatga agctgcgcgc
     2401 cgcctggccg ccttccacgc catcggtgca tgtccacaga cccgcgtcct ggaacgcgaa
     2461 ggatccagtg tcggtccatg tcagggtcat ggcaccgcgc acgaggtacc gccggtgcac
     2521 gcagcggatc agcccggtcg cgggcggcgt gtcgtacgcg gcgacggcga cgctgccgag
     2581 gcacacgaag tgggccggtg ggatcgggtt ccagatcgtg acgtcgtgat cgccgaggct
     2641 gccctcgtcg gtccagatct gctcgtaacc gaccggctcg cgcacagccc cgggatcgtc
     2701 gcggatcacc agcgtggcga agcccggcgc gtcgtggctc gcctgcgcga cgtcaccgac
     2761 cgacacaaaa cccgcgggca cgaccggacg ccagatcgat ccatcggtca gcgcaccgct
     2821 gccgctgtcg tcataaaccc agacgtagtc gctgaccggc agcagctcga gcggcctggc
     2881 cggctcgtcg ggcgggtcga cgaacgcgag cgcctcgtgc accgggctgc cgagccagct
     2941 cgcgggcgcc gcgagaccgt gcagcgccgc gaccgtcgcc gcgatgtcga agatccgcgt
     3001 ctcacgctgg agctgcacgc cagcgggtac ctgcggcccg gtgacgatca acgggatcgg
     3061 ccgctcgagc gaggtgtcgg ccccgtgcga caggccgtcg ccgccgtgat cggcgctgac
     3121 cagcaccgcg gtgtacgggc gcatgccggc ggcgtcgatg cccgcgagca cctcgccgac
     3181 cagcgcatcg gcgcttccgg ccgcctcgac gtacgcgtcg gagccccagc cgttgaagtg
     3241 cccggcgtga tcgacgaggt cgaggtggat gaacagcagg tcgggcgcgt tgccctgcat
     3301 ccacgccagc gcggcgctca cggtctcgac ctcgtcgccg gggctcgtga tcacgtcggc
     3361 gacgccgggc tcgacgagcg cgccgaagcc gtcccagtcg tggaacaccc cgatcctggc
     3421 ggtcggtcgt tgcgcgcgca gcacggcgaa gatcgtcggg gtcggctgcg agtcgccggg
     3481 ctcccactcg ttcgagtcga cgccgtgctg atcggcggtc gagccaacga tcatcgacat
     3541 ccagttcggc gcgctcatcg tcggcagcgt gttctgcatc gccagcgtcg acacgcccgc
     3601 ctgcatcatg ccatcgagca ccggggtcgc cacctcgggc acgtaggccc caccgaggcc
     3661 gtcgatgccg aggacgacca cgtgccggat cggcgtcacg caggtgacca cgatgtcgcc
     3721 gacgtcggcg ccggcgacca agccctcacc gtgagcgatc atgcaggtct gatcgggcgc
     3781 gccgggctgc gtggcgacgg tgacgctgta gctcgcgccc tcgcccgcgg gcgtgacgaa
     3841 gtcgaaggga ccgtcgccag cgatcgccag aacctcgccc gcgagcgcca gctcgagccc
     3901 cgcgccttgc aggccgcgca cgctgccgcc gatcgtgtgc gtcgcgacgc cggtcggctc
     3961 gcccgtgtcg ctgccggtcg cggtcggcgc cccgccagtg gcctcgccgg tcccggtcgg
     4021 atcgacgccc gctgacgatc cgccgctcgt gcccgtcgat gcccccggcg agctcccgct
     4081 gccctcacca ggctcaccgc aggacatcgt cagcccgccc aggaacacga tcgtacgtgc
     4141 acgcatgcgc ctgcacggtc ccacggacgg ccggcccgca ccagttcctg cccgacagcc
     4201 gacacgcgcg accgcgttcg aaatttcgcg cctgcgcgcg agggcggcgc ggctccggct
     4261 cgcgtatgct cgccgccatg tcgacgcgcc tccggctctc gttcgccctg tgtgctccgc
     4321 tgctcgcctc gtgtggcggt gaaccatcga ccaccgatac gggggacgcc tccaccagcg
     4381 ccagcgccga cacctccacg ggcgacatcc ccgtcgaccc gaccggcgcc ccgaccacca
     4441 ccggcgccag caccaccgac acccccgaca ccaccgacac cagcggcggc gaggtccacg
     4501 tcgtcccgca gttcggcggc acctttacgg tgatcggcac cgcggcggac ggcctcaacg
     4561 tcgtgcgcga cctcgagttc cacccgggcg cgcccgacca gctgtggacc tacaacatgc
     4621 tcagccacgg cgtcgtgatc ttcttcgcgc ccggcagcgc ggagcagacc tccgaggtcc
     4681 gcatcgacgc ctacggccag cacttcatgg cctacgtgtc gtcggcggcc ttcggcgtcg
     4741 acggcaagtt cgcgacctgt caggagtcgc gcaacgagtg gaacgtcggg ccgcaaaagc
     4801 cggacgactt catgggtccg gccctgtggt cggccgacct ggacatcttc gccaaggtcc
     4861 accaggaggt cccgcccaac ccgctcgagg gcagccacct cgacatgctg caccagagcc
     4921 cgctgtgcat gggcatcgcc cacgacaccg ccaacgtgta ctggatcttc gacggcaaga
     4981 acagtgacat cgtgcgctac gacttccaga ccgatcacgg acccggcggc ggcgaccact
     5041 cggacggtgt catcacccgc tattacgacg ccgaggtgac gcggatcgcc aacatcccgg
     5101 gccacctgga gatcgaccac gcaaccggca tgctctacgt cgccgacacc ggcggcggac
     5161 ggatcatgcg
//
LOCUS       DASWNJ010000642         2921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_12286, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000642 DASWNJ010000000
VERSION     DASWNJ010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12286"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1370)
                     /locus_tag="VGB85_10790"
     CDS             complement(<1..1370)
                     /locus_tag="VGB85_10790"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0134562.1"
                     /translation="MGSPDVIALPAALTSGLGGSSGWPSGPSSGAGATEDEHNTAAGS
                     NSAAGHAAPDELAPGTAVGRYMILSRLGAGAMGVVYAAYDPELDRKVALKLLQPRGGS
                     SLDSRIRLMREAKALARLSHPNVVAVHDVGTFAERVFLAMEFVDGKTLGAWLKERPQR
                     WPDVLAIMRKAGEGLAAAHAAGLVHRDFKPDNVLIARDGRVRVLDFGLARSAGEVLPE
                     ERDPAAAAILASVSSERRGQSQMEAAMMTRTGAVVGTPAYMSPEQHLGRSADARSDQF
                     SFCVAMYQALYGARPFVGERMSSLAFPVLQGKIAPAPGGSAVPMWLRKVVLRGLQVDP
                     DKRYPGMPALLADLNRDDGRGRRRGWFVASSAIATLGLGLGLRASAPAVEAPCQGARD
                     RLAGIWDEPTIADVERAFRSSGLPYAAETWTGVRASLDAHAAAWTGMHTDSCAATRIR
                     GEQSDT"
     gene            1454..2218
                     /locus_tag="VGB85_10795"
     CDS             1454..2218
                     /locus_tag="VGB85_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134563.1"
                     /translation="MRDEHLTPVLEACTEAELALLIEFLGRPPSGLMWLDRRVREPGH
                     SHEERVAAVVAEILRFGNHSVAGRIGAGTPGWLQVVCDVLQNLELSEAPAPGILELEM
                     RVVRFVLDTEFDRLSPELQVHLMRGFYAGEFFVGGLRGYEPLHPFLTRVDADHRALGG
                     AKMARAMRTVGLRELRKRASSFARKTALKVVLRRFAGPVQWVLTAWEWLGPAYRITVP
                     VVCYVAYLRHAQEARRAEQAEKTSGEGDADQALAAS"
     gene            complement(2197..>2921)
                     /locus_tag="VGB85_10800"
     CDS             complement(2197..>2921)
                     /locus_tag="VGB85_10800"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0134564.1"
                     /translation="GCALACAFCATGRMGLRRNLEAWEIVEQVRLVRERLPPDGRVRG
                     VVFQGMGEPLANLDRVLAAISVLSDPSALAIDARAITVCTSGLPAGIRRLARESPMVR
                     LGLSIASARRDLRRHLMPIDAAHPLPEVITAAVEHAQLTGLAPMFAVTLLRGHNDGEA
                     DALALADLVHDFTARAGLRPRLTILAYNTIATEDDPFTRTDEAVESRFRDILAGRGVF
                     THRRYSGGGDVDAACGQLAARA"
BASE COUNT          398 a          981 c         1164 g          378 t
ORIGIN      
        1 aaggtgtcgg actgctcgcc gcggatccgg gtcgcggcgc acgagtcggt gtgcatgccg
       61 gtccaggccg cggcgtgggc gtcgaggctg gcgcgcacgc cggtccaggt ctcggccgcg
      121 taggggaggc ccgaggaccg gaatgcgcgc tcgacgtcgg cgatcgtcgg ctcgtcccag
      181 atgccggcga ggcggtcgcg cgcgccctgg cacggagcct cgacggccgg ggccgaggcg
      241 cgcaggccca ggccgaggcc gagcgtggcg atcgcgctgc tggcgacgaa ccagccgcgc
      301 cggcgcccgc ggccgtcgtc gcggttgagg tcggcgagca gcgcgggcat gccggggtag
      361 cgcttgtcgg ggtcgacctg taagccgcgc agcacgacct tgcgcagcca catcggcacg
      421 gcgctgccgc ccggggccgg cgctatcttg ccctgcagga ccgggaacgc gaggctcgac
      481 atgcgctcgc cgacgaaggg gcgcgccccg tagagcgcct gatacatggc gacgcagaac
      541 gagaactgat cggagcgggc gtcggcgctg cggccgaggt gctgctccgg ggacatgtac
      601 gcgggcgtgc cgacgacggc gccggtgcgg gtcatcatcg cggcttccat ctgcgactgc
      661 ccgcgccgct cgctgctgac ggaggcgagg atcgcggccg cggccgggtc acgctcctcg
      721 gggagcacct cgccggcgct gcgggccagg ccgaagtcga ggacgcggac gcggccgtcg
      781 cgggcgatca gcacgttgtc gggcttgaag tcgcggtgga ccaggccggc ggcgtgggcc
      841 gccgcgaggc cctcaccggc cttgcgcatg atcgccagga cgtcgggcca acgctgcggg
      901 cgctccttga gccaggcgcc gagggtcttg ccgtcgacga actccatcgc caaaaacacg
      961 cgctcggcga aggtgccgac gtcgtgcacg gcgacgacgt tggggtgcga gaggcgggcg
     1021 agcgccttgg cctcgcgcat caggcggatc cggctgtcga ggctgctgcc gccgcgcggc
     1081 tgcaacagct tgagggcgac cttgcggtcg agctcggggt cgtaggcggc gtacacgacc
     1141 cccatcgcgc cggcgccgag tcgactgagg atcatgtagc gaccgacggc ggtgccgggc
     1201 gccagctcgt cgggcgccgc gtgcccggcg gcggagttcg agcccgcggc cgtgttgtgc
     1261 tcgtcctcgg tggcgccggc tccgctgctc ggcccgctcg gccagcccga cgagccgccg
     1321 agaccggagg tcagggcggc aggcagggcg atgacgtcgg gcgagcccac gagaccgccg
     1381 atcttgcccg agcgccgcgg ggtactcaat ggctgcgagc gtgggatgga tcgcgtaagc
     1441 tcgcggcgct ctcatgcgcg atgaacacct gacgccagtg ctggaggcct gcaccgaggc
     1501 cgagctcgcc ctcctgatcg agttcctcgg ccggccgccc tcggggctga tgtggctcga
     1561 ccgccgcgtg cgcgagccgg gacacagcca cgaggagcgc gtcgcggcgg tggtcgcgga
     1621 gattctgcgc tttggcaacc acagcgtcgc cggccggatc ggcgcgggca ccccgggctg
     1681 gttgcaggtg gtgtgcgacg tgttgcagaa cctcgagctg tcggaggcgc cagcccccgg
     1741 gatcctcgag ctcgagatgc gagtggtgcg gttcgtgctc gacaccgagt tcgatcggct
     1801 gtcgcccgag ctgcaggtgc acctgatgcg gggcttctac gccggagagt tcttcgtcgg
     1861 cggactgcgc gggtacgagc cgctgcaccc gttcctgacc agggtcgacg cggaccaccg
     1921 ggccctcggc ggcgccaaga tggcgcgcgc gatgcgcacg gtcggccttc gcgagctgcg
     1981 caagcgggcc agcagcttcg cccgcaagac ggcgctcaag gtcgtgctgc gccgcttcgc
     2041 cggaccggtg cagtgggtgc tgaccgcgtg ggagtggctc ggcccggcct atcgcatcac
     2101 ggtgccggtc gtctgttacg tcgcgtacct gcggcacgcg caggaggcca ggcgcgccga
     2161 gcaggcggag aagacctcgg gcgagggcga cgcggatcag gccctcgcgg cgagctgacc
     2221 gcaggcggcg tcgacgtccc caccgccgct gtagcgccgg tgggtgaaca cgccgcgccc
     2281 ggcgaggatg tcgcgaaagc gcgactcgac ggcctcgtcg gtgcgcgtga aggggtcgtc
     2341 ctcggtggcg atcgtgttgt aggcgaggat ggtcaggcgg gggcgcaggc cggcgcgggc
     2401 cgtgaagtcg tgcacgaggt cggcgagcgc gagcgcgtcg gcttcgccgt cgttgtggcc
     2461 gcgaaggagg gtaacggcga acatcggcgc gaggccggtc agctgcgcgt gctcgacggc
     2521 ggcggtgatg acctcgggca gcggatgcgc cgcgtcgatc ggcatcaggt gacgtcgcag
     2581 gtcgcggcgg gcgctggcga tcgagaggcc gaggcgcacc atcggcgact cgcgggcgag
     2641 gcgacggatc ccggcgggca agcccgaggt gcacacggtg atcgcccgcg cgtcgatagc
     2701 gagcgcggag ggatcggaga gcaccgagat cgccgcgagc acgcggtcga ggttggcgag
     2761 cggctcaccc atgccctgaa acacgacgcc gcggacgcga ccgtcgggcg gcaggcgctc
     2821 gcggacgagg cggacctgct cgacgatctc ccacgcctcg aggttgcgcc gcaggcccat
     2881 gcgaccggtg gcacaaaagg cgcacgcgag cgcgcaacca a
//
LOCUS       DASWNJ010000643         4801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_81783, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000643 DASWNJ010000000
VERSION     DASWNJ010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81783"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VGB85_10805"
     CDS             <1..339
                     /locus_tag="VGB85_10805"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEY0134565.1"
                     /translation="ETVHRAGRKVLWMCDPMHGNTHSTASGLKTRDFDDILREIERSF
                     AVHRELGTVLGGIHFELTGEDVTECVGGGITEADLSRNYASVCDPRLNYRQSLEMAFR
                     VGRWIGKSSR"
     gene            complement(353..2161)
                     /locus_tag="VGB85_10810"
     CDS             complement(353..2161)
                     /locus_tag="VGB85_10810"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HEY0134566.1"
                     /translation="MPCSRPPLQLLAALALTTACGDAAQTTGTTDITAGTTDAGTTSG
                     TTPGGTTGDATSEPTTTPTTSPTGTTDITAGTDGESSSGMSCPANAPPTTPIVVEPLA
                     GRIDVIPATLKIQGSPFIDPDAGDVQGGAEVEIWRVKDGIVDERVWHAELTGEGPVDV
                     TLADGEFDDASDDTLADWKDHVVRLRYRDEHGPCSAYGEWSPDIAFRTDDGSTALFDD
                     AKILDFYIDIPPASWDKINDEAIPPGCVPYARNYYTGTLRHGDQTFPGVGVKVKGGCG
                     SSRDLNGKASFKINLEWDDPDVAGCPGERRLMGEKSFTFNNGVQDQSASNERIGYPIF
                     RELGIPAPRAASVRIFVNDDLWGLYTHVETIDRRFLSRWHASNDGMLYEGTYWCDLIP
                     DNVPPTDDDDSYCITREFSPDACSTPEPGADPEDYTLIRDLVEKIDALPPGGFYPAVE
                     TFFDYDKFLTTWAIEAVIDHWDNYAFAIKNNYRVYHDPISDRWSMITTGIDQTFEKDQ
                     DAWGAQGVLAARCLEEPACEAAFAARLDEVNEAIAMMDLAGRAEAIHDQISAAVMADP
                     RKEYNMKTFDQAHQAVLAFIAGRADEIRDQLMNHGF"
     gene            complement(2287..3543)
                     /gene="hmpA"
                     /locus_tag="VGB85_10815"
     CDS             complement(2287..3543)
                     /gene="hmpA"
                     /locus_tag="VGB85_10815"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: protein motif:HMM:NF009805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HEY0134567.1"
                     /translation="MLSANTIEIVKATAPAVAAHAEEITRCFYKKMFAGDPQVKAFFN
                     QAHQHSGGQQRALAGAICAYAANIDNLGVLGPAVEVIAQKHGSLLIQPEHYPIVGKHL
                     LVAIKEVLGDAATDEVIAAWGEAYGFLAQILIDRERAIYAAQAAAPGGWVGRRPFVVA
                     RKVVESDIVTSFYLKPQDGGALPPFKPGQYITVHVDHPTTPTSPRNYSLSDRPGQDHY
                     RISVKREPGTPPGLISNYLHEQVNEGDVLQLGPPCGEFTLDVDAVHKPIVLISGGVGL
                     TPMLSMLKTLVHRGTDAPIYFVHGTRNSSTHALAGEVEEIAAAHPNVHVHVRYSDPLP
                     TDDTSGHAHGTGIIDVALLEKLLPSSDAEYYLCGPKPFMVCVHEGLQRRGVPEAQIHF
                     EFFGPKQDLVISTTDAADAAEAAQAV"
     gene            complement(3646..>4801)
                     /locus_tag="VGB85_10820"
     CDS             complement(3646..>4801)
                     /locus_tag="VGB85_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134568.1"
                     /translation="GTVYAPEGTLPLYNVVVYVPNGPLDPIPDGVTCDTCQTGLSGQP
                     ITATLSDTKGEFILEGVPAGANIPLVIQIGKWRREVVVPNVEPCAETVVAPDLTRLPR
                     NQGEGHIPRIAMVTGSADALECLLRKIGLEDQEFTPEASQGRINMFAGDGGGDQYVND
                     LNNGENFPNGKSLWDDLPKLKNYDLVLMACEGGTDEGNKSDQARQNIVDYAGVGGRLF
                     FSHWHNIWIEGGADPFPSAATFDHQDDLESPFTAKIDMTFPKGMALAQWLVNVAGSTQ
                     LGEIAITAGQNTVAAINDAVSTRWIYGEDPTSIQYFTFNAPIGAPAEQQCGRVVDTDI
                     HVSSGDDPGNSFPSGCTTNDLSPQEKVLIFMLFELSSCLTPDDEPPPIPG"
BASE COUNT          662 a         1492 c         1820 g          827 t
ORIGIN      
        1 gagacggtcc accgcgcggg ccgcaaggtg ctgtggatgt gcgacccgat gcacggcaac
       61 acccactcga ccgcgagcgg cctcaagacc cgcgacttcg acgacatctt gcgggagatc
      121 gagcggtcgt tcgcggtcca ccgcgagctc ggcacggtgc tcggcggcat ccacttcgag
      181 ctgaccggcg aggacgtcac cgagtgcgtc ggcggcggca tcaccgaggc cgacctctcg
      241 cgcaactacg cgagcgtctg cgacccccgc ctcaactatc gccagtcgct cgagatggcg
      301 tttcgcgtcg ggcggtggat cgggaagtcg agccgctgag cggcccggcg gctcagaagc
      361 cgtggttcat cagctggtcg cggatctcgt cggcgcgacc ggcgatgaac gcgagcacgg
      421 cctggtgggc ctgatcgaag gtcttcatgt tgtactcctt gcgcgggtcc gccatgaccg
      481 cggcggagat ctggtcatga atggcctcgg cgcggccggc gaggtccatc atggcgatcg
      541 cctcgttgac ctcgtcgagg cgggcggcga aggcggcctc gcaggcgggc tcctcgaggc
      601 agcgggccgc gagcacgccc tgggcgcccc aggcgtcctg atccttttca aaggtctggt
      661 cgatgccggt cgtgatcatc gaccagcggt ccgagatcgg gtcgtgatag acgcggtagt
      721 tgttcttgat cgcaaacgcg tagttgtccc agtgatcgat gacggcctcg atcgcccagg
      781 tggtcaggaa cttgtcgtag tcgaagaacg tctcgacggc ggggtagaag ccgccgggcg
      841 gcagcgcgtc gatcttctcg acgaggtcac ggatcagggt gtagtcctcc gggtcggcgc
      901 cgggctcggg ggtcgagcag gcgtcgggcg agaactcgcg cgtgatgcag tacgagtcgt
      961 cgtcgtcggt cggcggcacg ttgtccggga tgaggtcgca ccagtaggtg ccctcgtaga
     1021 gcatgccgtc gttgctggcg tgccagcgcg acaggaaccg gcggtcgatc gtctcgacgt
     1081 gggtgtagag gccccagagg tcgtcgttga cgaagatgcg gacgctcgcg gcccgcggcg
     1141 ccgggatccc gagctcgcgg aagatcgggt agccgatccg ctcgttgctc gccgactggt
     1201 cctggacgcc attattgaag gtgaagctct tctcacccat caggcggcgc tcgcccgggc
     1261 agccggcgac gtcggggtcg tcccactcga ggttgatctt gaacgaggcc ttgccgttga
     1321 ggtcgcgcga cgagccgcag ccgcccttga ccttgacgcc gacgccggga aacgtctggt
     1381 cgccgtggcg cagggtgccg gtgtagtagt tgcgcgcgta ggggacgcag ccgggcggga
     1441 tcgcctcgtc gttgatcttg tcccagctcg cgggcgggat gtcgatgtag aagtcgagga
     1501 tcttcgcgtc gtcgaacagc gcggtgctgc cgtcgtcggt gcgaaacgcg atgtccgggc
     1561 tccactcgcc gtacgcgctg cacgggccgt gctcgtcgcg gtagcgcagg cggacgacgt
     1621 ggtccttcca gtcggcgagc gtgtcgtcgg aggcgtcgtc gaactcgccg tcggcgaggg
     1681 tgacgtcgac cggaccctcc ccggtcagct cggcgtgcca cacccgctcg tcgacgatac
     1741 cgtccttgac gcgccagatc tcgacctccg cgccgccctg cacgtcaccc gcgtcggggt
     1801 cgatgaaggg cgagccctgg atcttcaggg tcgcggggat gacgtcgatg cggccggcca
     1861 gcggctcgac gacgatcggc gtcgtcggcg gagcgttggc cgggcagctc atgccgctgc
     1921 tgctctcgcc gtcagtgccg gcggtgatgt cggtcgtgcc cgtcgggctc gtggtcggcg
     1981 tggtcgtcgg ctcgctggtc gcgtcgcccg tggtgccacc cggggtcgta ccggaggtgg
     2041 tgcctgcgtc ggtggtgccg gcggtgatgt cggtggtgcc cgtggtctgg gccgcgtcgc
     2101 cgcaggcggt ggtgagcgcg agggcggcga gcagttgcaa gggcgggcgg gagcagggca
     2161 tcgggatcct cgcgtgaacg ggtcgtggcg cgccgctgcg cagcggcgcg ggcagacatg
     2221 ctaccccgcc gggccgcgcg tgtgcgagcc cgggcgggga cgttcacgag cggcgccgcg
     2281 gcctgctcag acggcctgcg cggcctcggc tgcgtcggcg gcgtcggtcg tcgagatcac
     2341 gaggtcctgc ttcggtccga agaactcgaa gtggatctgc gcctcgggca cgccccggcg
     2401 ctgcaggccc tcgtgcacgc acaccatgaa gggcttgggg ccgcacaggt agtactcggc
     2461 gtcgctgctc ggcagcagct tctcgagcaa cgcgacgtcg atgatgccgg tgccgtgggc
     2521 gtggccgctg gtgtcgtcgg tcggcagcgg gtcgctgtag cggacgtgca cgtggacgtt
     2581 cgggtgcgcg gccgcgatct cctcgacctc gccggcgagc gcgtgcgtgc tgctgttgcg
     2641 ggtgccgtgg acgaagtaga tcggggcgtc ggtgccgcgg tgcacgaggg tcttgagcat
     2701 cgacagcatc ggggtcaaac cgacgccgcc gctgatcaac acgatcggct tgtgcacggc
     2761 gtcgacgtcg agcgtgaact cgccgcacgg cgggccgagc tggagcacgt cgccctcgtt
     2821 gacctgctcg tgcaggtagt tcgagatcag gccgggcggg gtgccgggct cgcgcttgac
     2881 gctgatgcga tagtggtcct ggccgggccg gtccgacagg ctgtagttgc gcggcgaggt
     2941 cggcgtggtc gggtggtcga cgtgcaccgt gatgtactgc cccggcttga acggcggcag
     3001 cgcgccgccg tcttgcggct tgaggtagaa cgaggtgacg atgtcgctct cgacgacctt
     3061 gcgcgcgacg acgaacggcc ggcggccgac ccagccgccg ggagccgcgg cctgcgcggc
     3121 gtagatcgcc cgctcgcggt cgatgaggat ctgcgcgaga aagccgtagg cctcgcccca
     3181 cgcggcgatg acctcgtcgg tggcggcgtc gccgagcacc tccttgatcg cgacgagcaa
     3241 gtgcttgccg acgatcgggt agtgctcggg ctggatcagc agcgagccgt gcttctgggc
     3301 gatgacctcg acggcggggc cgagcacgcc gaggttgtcg atgttggccg cgtaggcgca
     3361 aatggcaccg gcgagggcgc gctgctgacc gcccgagtgc tggtgggcct ggttgaagaa
     3421 cgctttgacc tgcgggtcac ccgcgaacat cttcttgtag aagcaccggg tgatctcctc
     3481 ggcgtgggct gcgaccgccg gagcggtcgc ctttacgatc tcgatcgtgt ttgcgctcaa
     3541 catgggcaga ggcttaaccc aggccgaaga tctgggcacg cgcatcgtgg gcacgggccg
     3601 gcgccaaaat tgccgagtga gtaagccgag tgagccaact agcggtcagc cggggatcgg
     3661 cggcggctcg tcgtcggggg tcaaacacga gctgagctcg aacagcatga agatgaggac
     3721 cttttcctgc ggggacaggt cgttggtcgt gcaaccggac gggaacgagt tgccggggtc
     3781 gtcgccggag gagacgtgga tgtcggtgtc gacgacgcgg ccgcactgct gctccgcggg
     3841 cgcgccgatc ggggcgttga aggtgaagta ctggatcgag gtcgggtcct cgccgtagat
     3901 ccagcgcgtc gagacggcgt cgttgatcgc ggcgacggtg ttctggccgg ccgtgatcgc
     3961 gatctcgccg agctgcgtcg agccggcgac gttgacgagc cactgcgcga gcgccatgcc
     4021 cttggggaag gtcatgtcga tcttggcggt gaaggggctc tcgaggtcgt cctggtggtc
     4081 gaaggtggcc gcgctcggga acgggtcggc gccaccttcg atccagatgt tgtgccagtg
     4141 cgagaagaac aggcgcccgc cgacgcccgc gtagtcgacg atgttctggc gggcctggtc
     4201 cgacttgttg ccctcgtcgg tgccgccctc gcaggccatc agcacgaggt cgtagttctt
     4261 gagcttgggc aggtcgtccc acaggctctt gccgttgggg aagttctcgc cgttgttgag
     4321 gtcgttgacg tactggtcgc cgccgccgtc gccggcgaac atgttgatcc ggccctggct
     4381 ggcctccggg gtgaactcct ggtcctcgag gccgatcttg cgcagcaggc actcgagcgc
     4441 gtcggcgcta ccggtgacca tggcgatccg cgggatgtga ccctcgccct ggttccgcgg
     4501 caggcgggtg aggtcggggg cgacgacggt ctcggcgcag ggctcgacgt tcggcacgac
     4561 gacctcgcgg cgccacttgc cgatctggat cacgagcggg atgttggcgc cggccggcac
     4621 accctcgagg ataaactcac ccttggtgtc gctcagcgtc gcggtgatcg gctggcccga
     4681 gaggccggtc tgacaggtgt cgcaggtgac accgtcgggg atcgggtcga gcgggccgtt
     4741 cgggacgtag acgacgacgt tgtagagcgg cagcgtgccc tcgggcgcgt acacggtgcc
     4801 c
//
LOCUS       DASWNJ010000644         3542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_647388, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000644 DASWNJ010000000
VERSION     DASWNJ010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647388"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VGB85_10825"
     CDS             complement(<1..679)
                     /locus_tag="VGB85_10825"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY0134569.1"
                     /translation="MKLGPAILLASALAGCGRVAPPVSITETRKIERGQTTCEGVSFA
                     VGCGDRKCDARGGETTTTCPSDCVVAKLVSYNEQTLCDSVHEVVEPTDTAGVAAVIRR
                     ARAEGRQVRVGGRQHTSNGQLCTSGIIVSTARMKHALHIERFEGFETVVTEPGIRMGD
                     LTDWLDARGRSLGFAVLGTRDPTIAGAIATGSHGSSPQHNSVVSNLVQSIELVDANGD
                     VREYTRGT"
     gene            867..1379
                     /locus_tag="VGB85_10830"
     CDS             867..1379
                     /locus_tag="VGB85_10830"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0134570.1"
                     /translation="MTAFEIFVTKEAMDAASLYLVGKKLAVLAEQAMATPEGLPAEPT
                     TERLVLRALAERLVLRAVAERPGLTVGELARQLSIAQSRISQVVAVLEREGFVRRYTD
                     AHDRRRQRIEATAQYKRMVEQRMMRQAEEALGPLLTHATAREKARVFAALDLIHELIL
                     RTEGREDVGG"
     gene            1424..2452
                     /locus_tag="VGB85_10835"
     CDS             1424..2452
                     /locus_tag="VGB85_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0134571.1"
                     /translation="MRYNLLGKTGIYVSEICLGTMTFGGKGFWEVVGSLGTQEVEAIV
                     GTALDAGVNFIDTANVYSEGESERLLGAALKSLGRPRDQVVVATKVRGRMGPGPNQVG
                     LSRAHILASIDHSLARLGLDHVDLYQIHGVDRTTPIEETVRALDDVVRAGKARYVGFS
                     NLPAWLAMKAIGFAEANGLARFESAQVYYSIAGRDIEREIVPLAQSERVAILPWSPLA
                     GGLLSGKFDPNKVGPEGARRTQFDFPPVDPDRLPRVLAALHAVAAATGVSVARIALAW
                     QLTRPFVTSVLIGAKNREQLTDNLQATSVELSPEHVQQLDDASALPAEYPGWMVDWQN
                     REPRVPNS"
     gene            complement(2475..3461)
                     /locus_tag="VGB85_10840"
     CDS             complement(2475..3461)
                     /locus_tag="VGB85_10840"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0134572.1"
                     /translation="MTTTLRRLGRQGPQVFPLALGCMGMSPMYGATDERESVATIHAA
                     IDRGINLLDTGDFYASGHNEMLVGRALADRRDRAMLSVKFGALRTPGGGWGGVDGRPA
                     AVKNFAAYSLQRLGVDHIDIYRLARLDPTVPIEDTIGAIAELVTSGHVRHIGLSEMGA
                     DTIRRAAAVHPIVDLQIEYSLVSRKPEVEILPTLAELGVGMTAYGVLSRGLLAGSLPK
                     GPRDFRSHLPRFTGDNLEKNQRLVETLAGLASARGVTSSQLAIAWVLARGEHIVPVIG
                     ARTRAQLDDALAALDLQLGADDLAEIEAAIPADAIAGDRYDAHQMQALDSER"
BASE COUNT          518 a         1127 c         1361 g          536 t
ORIGIN      
        1 cggtgccacg ggtgtactcg cggacgtcac cgttggcgtc gacgagctcg atcgactgca
       61 cgaggttgga cacgacgctg ttgtgctgcg gcgagctgcc gtgcgaaccg gtggcgatcg
      121 cgccggcgat cgtcgggtcg cgtgtgccga gcacggcgaa gccgagcgag cgcccgcgtg
      181 cgtcgagcca gtcggtcagg tcgcccatgc ggatcccggg ctcggtcacg accgtctcga
      241 agccctcgaa gcgctcgatg tgcagcgcat gcttcatgcg cgcggtcgag acgatgatcc
      301 cggaggtgca cagctggccg ttcgaggtgt gctgtcggcc gccgacgcgg acctggcggc
      361 cctcggcgcg tgcgcggcgg atcacggcgg ccaccccggc ggtgtcggtc ggctcgacga
      421 cctcgtgcac gctgtcacac agcgtctgct cgttgtagga gaccagcttg gcgaccacgc
      481 agtcggacgg gcaggtcgtc gtggtctcgc cgccccgcgc gtcgcatttg cgatcgccgc
      541 agccgacggc gaacgatacg ccctcgcagg tggtttgtcc gcgctctatc ttgcgtgtct
      601 cggtgatcga gacgggtggg gcgacgcgcc cgcagcccgc gagggccgat gcgagtaaga
      661 ttgccgggcc gagtttcatc gtgaattcgt gcgggtgaac ggggacacgt ggatgtgagg
      721 cgagcggagc ggacagcgcc gggatgctca catatctcta aaaatacatc tcaaaagata
      781 tatcgtcaag ttcggggtcg tggcgaccgt cgctgcgggc gccggcatcg ctggagtgcg
      841 tggcgcgcgc ggcggggagg cccgccttga cagcgttcga gatcttcgtg actaaggaag
      901 cgatggacgc ggcttcgctt tatctcgtcg gtaaaaagct ggccgtgctc gccgagcagg
      961 cgatggcgac cccggagggg ctgccggccg agccgacgac ggagcggctg gtgttgcgcg
     1021 ccctggccga gcggctggtg ctgcgcgccg tggccgagcg ccccgggctg acggtcggtg
     1081 agctcgccag gcagctatcg atcgcgcaga gccggatctc ccaggttgtg gccgtgctcg
     1141 agcgcgaggg cttcgtccgc cgctatacgg acgcgcacga ccggcgcagg cagcgtatcg
     1201 aggcgaccgc gcagtacaag cgaatggtcg agcaacggat gatgcgccag gcggaggagg
     1261 cgctggggcc tctgctgacc cacgccaccg cgcgggagaa ggcccgcgtg ttcgcggcgc
     1321 tggatctgat ccacgagttg atcctgcgga ccgaggggcg cgaggacgtg ggcggctgag
     1381 gccacgctcg ccgggggctc ccgcggcggt gtaccatgcc gacatgcgct acaacctgct
     1441 cggcaagacc ggtatctacg tctcggagat ctgcctgggg acgatgacct tcggcggcaa
     1501 ggggttctgg gaggtggtcg ggagcctcgg gacccaggag gtcgaggcga tcgtcggcac
     1561 cgcgctcgac gcgggcgtca acttcatcga cacggcgaac gtgtactcgg agggcgagtc
     1621 ggagcggctg ctcggcgcgg ccctcaagtc gctcgggcgc ccgcgcgatc aagtggtggt
     1681 ggcgaccaag gtccgcggtc gcatgggccc ggggccgaac caggtcgggc tgtcgcgcgc
     1741 gcacatcctg gcgagcatcg accacagcct ggcgcgcctc gggctcgatc acgtcgacct
     1801 gtatcagatc cacggggtcg accggacgac gccgatcgag gagacggtgc gcgcgctcga
     1861 cgacgtcgtc cgcgcgggca aggcccgcta cgtcggcttc agcaacctcc cggcgtggct
     1921 ggcgatgaag gcgatcggct tcgccgaggc caacggcctg gcccgcttcg agagcgcgca
     1981 ggtgtattac tcgatcgccg gtcgcgacat cgaacgcgag atcgtgccgc tggcacagtc
     2041 cgagcgcgtg gcgatcctgc cgtggagccc gctcgcgggc gggctgctgt ccggcaagtt
     2101 cgacccgaac aaggtcgggc ccgagggggc gcggcgcacc cagttcgact tcccgccggt
     2161 cgaccccgac cgtctgccgc gcgtgctcgc ggcgctgcac gccgtcgccg cggcgacggg
     2221 ggtgtcggtc gcccgcatcg ccctcgcgtg gcagctgacg cggccgttcg tcaccagcgt
     2281 gctgatcggc gcgaagaacc gcgagcagct gaccgacaat cttcaggcga cgagcgtcga
     2341 gctctcgccc gagcacgtgc agcagctcga tgatgccagc gcgctgccgg ccgagtaccc
     2401 gggctggatg gtcgactggc aaaatcgcga gccgcgggtg ccgaacagct gagcgctgat
     2461 cagcggatca tttgtcagcg ctcgctgtcg agggcctgca tctggtgggc gtcgtagcgg
     2521 tcgccggcga tcgcgtcggc cgggatggcg gcctcgatct cggcgaggtc gtcggcgccg
     2581 agctggaggt cgagcgcggc gagggcgtcg tcgagctgcg cccgggtgcg ggcgccgatc
     2641 accgggacga tgtgctcgcc gcgcgcgagc acccaggcga tcgccagctg cgagctggtc
     2701 acaccgcggg cgctggcgag gccggcgagg gtctcgacga ggcgctggtt cttctcgagg
     2761 ttgtccccgg tgaagcgcgg caggtgcgag cgaaagtcgc ggggaccctt cggcagcgag
     2821 ccggcgagca ggccgcggga cagcacgccg taggcggtca tgccgacgcc gagctcggcg
     2881 agggtcggaa ggatctcgac ctcgggcttg cggctgacca gcgagtactc gatctgcaga
     2941 tcgacgatcg ggtgcacggc cgcggcgcgc cggatcgtgt cggcgcccat ctcggagagg
     3001 ccgatgtggc gcacgtggcc ggaagtgacg agctcggcga tcgcaccgat cgtgtcctcg
     3061 atcgggacgg tcgggtcgag gcgcgcgagg cgatagatgt cgatgtgatc gacgccgagg
     3121 cgctggaggc tgtaggcggc gaagttcttg acggcggccg ggcgaccgtc gacgccgccc
     3181 cagccgcctc cgggcgtgcg cagggcgccg aacttgaccg agagcattgc ccggtcgcgg
     3241 cggtcggcga gcgctcgccc gacgagcatc tcgttgtgac ccgatgcata aaagtcgccg
     3301 gtgtcgagga ggttgatgcc gcggtcgatc gcggcgtgga tcgtggcgac ggactcccgc
     3361 tcgtcggtgg cgccgtacat cggcgacatg cccatgcaac cgagcgcgag cgggaagact
     3421 tgcgggccct ggcggccgag gcggcgaagt gtggtggtca tgacgcgtct cctgggaggc
     3481 accttactgt tcgccgggga ggcgataaag cgggggatcg tggacgcgct gttcgggcgg
     3541 cg
//
LOCUS       DASWNJ010000645         3259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_81797, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000645 DASWNJ010000000
VERSION     DASWNJ010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81797"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(344..1387)
                     /locus_tag="VGB85_10845"
     CDS             complement(344..1387)
                     /locus_tag="VGB85_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEY0134573.1"
                     /translation="MRRRLACSLVLSACTSLSACGGAGSSETTGQDSTTTGVTSGGIV
                     IPTTEPATSDGATTGPAPKLDLPPSADLPPAEGCGAVDILFVIDNSASMGQFQMALAD
                     AFPQFVDAMYANLPADISLHVGITTTDFYCTMPGEACCPSSCSIGNTGCQIGSTPEEV
                     AQLQTYYNPPTGGDNGVNGSQGRLFVHEDQPFFVSNTSDDSAPLKAWFTGAAIAAGEQ
                     GSSIEMPVAAAAFAVDPVNAATNAGFVRDNDSILLIVFLTNDPDASLQPLADYEAMVL
                     GAKQNCPECVLTAGLLKSCVPAEKQRLWQFMTAFGDQPIWRDIEAKSEYAEVVGGALA
                     SALVEACENIPVG"
     gene            complement(1494..2639)
                     /locus_tag="VGB85_10850"
     CDS             complement(1494..2639)
                     /locus_tag="VGB85_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134574.1"
                     /translation="MRITTPLLAACVVLAACNNKTDTKPNDGKVAETRTDAKPGTPTD
                     TATPATPTAPTTATAGTGDKFIPADAALLAHIDMKGLSGSPLWASNKAMMDADPEVKK
                     QMEAFSACNMPFDGFRTVDLGVGAEAKNIAVVAQGAGIGKPENLRCLSEKMKDGDWKL
                     EEVAGGKPRLVFDNGEAYGYLVDDDTLAIATKAWDASVTSLMTGKGTSVRDGALKDVL
                     AQADQTKHVWFAGNLPPQLAAMATAQPGMSGMAGLKSVAGSLDLTAGLGLVLAFGMEN
                     ADRAKTSLAEVQKQFASVKPMAGMLGIPATAVDKVTFEAKDAAVTMTASLTMDEINAM
                     SSAMKGAPGGAGAGAAGSTPTGTAPTDGSKIGTTNDKGAKPTPSTDM"
     gene            complement(2759..>3259)
                     /locus_tag="VGB85_10855"
     CDS             complement(2759..>3259)
                     /locus_tag="VGB85_10855"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0134575.1"
                     /translation="ILYQQGQYDDSVSAYRRALELLTRSLGGGHEDTLAVLLDLGRAL
                     HRLGDPAGALAAFQQNLDGVRATAGSEHPRVAGTLNAIGQVHYSQGDFKAALDHYHQA
                     LEIRQRTLGPDHWQTATSRFNCGTAMRELGDPWGQTEMQSAADTLERLLGPQHPHVLA
                     TRSWLR"
BASE COUNT          427 a         1015 c         1291 g          526 t
ORIGIN      
        1 ggccctgctc gggcgctgcg atctgcggct cgtcactctc cgcgggctcg tcgccgccgg
       61 gcggctgctg cacggggttg ttcctggcgg ggcagctggt caacaacagg ccgaggccgg
      121 agacgacgat cgggggggta cgcgtggtca tcgcagaagt ggctccatgc tcgcgggcgc
      181 gggcggtaag cacaagcccc gcggccgtac agcaaggaac gcgcgtcgat cgacgccgct
      241 tacaggcggg cgagcaactc gtcgagctcg gcccggactc gtcgcgggtg gcttgcagcg
      301 atcgaccgcg acggatcttc gcgcgcgatc gcccggcgcg tgatcagccg acggggatgt
      361 tctcgcaggc ctcgacgagg gccgaggcca gcgcgccacc gacgacctcg gcgtactcgg
      421 acttggcctc gatgtcgcgc cagatcggct ggtcgccgaa ggcggtcatg aactgccaca
      481 ggcgctgctt ctcggccggc acgcaggact tgagcaggcc ggccgtcagc acgcactccg
      541 ggcagttctg cttggcgccg aggaccatcg cctcatagtc ggcgagcggc tgcagcgagg
      601 cgtcggggtc gttggtgagg aagacgatca ggaggatcga gtcgttgtcg cggacgaagc
      661 cggcgttggt ggcggcgttg accgggtcga cggcgaaggc ggcggcggcg acgggcatct
      721 cgatcgacga gccctgctca ccggccgcga tcgcggcgcc ggtgaaccat gccttgagcg
      781 gggcggagtc gtcgctggtg ttgctgacga agaagggctg gtcctcgtgg acgaacagcc
      841 gaccctggct gccgttgacg ccgttgtcgc cgccggtcgg cgggttgtaa taggtctgga
      901 gctgggcgac ctcttcgggc gtgctgccga tctggcagcc ggtgttgccg atcgagcagc
      961 tcgacgggca acaggcctcg ccgggcatgg tgcagtagaa gtcggtggtg gtgatgccga
     1021 cgtgcaggct gatgtccgcc ggcaagttgg cgtacatggc gtcgacgaac tgcgggaagg
     1081 cgtcggcgag cgccatttgg aactggccca tcgaggcgga gttgtcgatc acgaacagga
     1141 tgtcgaccgc gccgcagccc tcggccggcg ggaggtcggc cgagggcggg aggtcgagct
     1201 tgggggccgg accggtggtc gcaccatcgc tggtcgcggg ctcggtggtc gggatgacga
     1261 tgccgccgct cgtcacaccg gtggtcgtgc tgtcttgacc ggtggtctcg ctcgagccgg
     1321 cgcccccgca ggcggacagc gaggtgcagg cggacaggac gagagagcag gcgaggcggc
     1381 gacgcacgcg ggcagtatgg cgcacttgcg ggcgcacgaa aacggccggc gcgcgggtgc
     1441 gcgaccggcc gttttgctgt caggcgaggg tccggtcgtg cgaccgggac cgctcacatg
     1501 tccgtggacg gggtcggctt ggcgcccttg tcgttggtcg tgccgatctt gctgccgtcc
     1561 gtgggggcgg tgccggtcgg ggtggagccc gcagcacccg caccggcgcc accgggggcg
     1621 cccttcatcg cggacgacat ggcgttgatc tcgtccatgg tcagcgaggc ggtcatcgtg
     1681 acggcggcgt ccttggcctc gaaggtgacc ttgtcgacgg cggtcgcggg gatgccgagc
     1741 atgccggcca tcggcttgac gctggcgaac tgcttctgca cctcggcgag gctggtcttg
     1801 gcgcggtcgg cgttctccat gccgaacgcg agcacgaggc cgaggccggc ggtcaggtcg
     1861 agcgagccgg cgacgctctt gaggccggcc atgccggaca tgccgggctg ggcggtcgcc
     1921 atcgcggcga gctgcggcgg caggttgccg gcgaaccaca cgtgcttggt ctggtcggcc
     1981 tgggcgagca cgtccttgag cgcaccgtcg cggacggagg tgcccttgcc ggtcatcagg
     2041 ctggtgaccg aggcgtccca cgccttggtg gcgatcgcga gggtgtcgtc gtcgacgagg
     2101 taaccgtagg cctcaccgtt gtcgaacacg aggcgcggct tgccgccggc gacctcctcg
     2161 agcttccagt cgccgtcctt catcttctcc gagagacagc ggaggttctc gggcttgccg
     2221 atgccggcgc cctgggcgac gaccgcgatg ttcttggcct cggcgccgac gccgaggtcg
     2281 accgtgcgaa agccgtcgaa gggcatgttg caggccgaga aggcctccat ctgcttcttg
     2341 acctcggggt cggcgtccat catggccttg ttggaggccc acagcggcga gccggacagg
     2401 cccttcatgt cgatgtgcgc gagcagcgcg gcatcggcgg ggatgaactt gtcgccggtg
     2461 ccggcggtcg cggtggtcgg cgcggtcggg gtggccggag tggcggtgtc cgtgggcgta
     2521 ccgggcttcg cgtcggttcg cgtctcggcg accttgccgt cgttcggctt ggtgtccgtc
     2581 ttgttgttgc aagcggcaag gacgacgcag gcggcgagca ggggggtggt gatgcgcatg
     2641 ggcggcggta tacggtggat cgcccggcgg cgcaatccgc ggacaggcgg cccgggaccc
     2701 cgcgcatgcg actttacgca gtacgcgtcg gctgccggcg gggccggcgg cggggcgcct
     2761 agcgcagcca ggagcgggtc gccaacacgt gtggatgctg cggcccgagc aggcgctcga
     2821 gcgtgtccgc ggcgctctgc atctcggtct gaccccaagg atcgccgagc tcgcgcatgg
     2881 cggtgccgca gttgaagcgt gaggtggcgg tctgccagtg atccgggccg agcgtgcgct
     2941 ggcggatctc cagggcctga tgatagtggt cgagggcggc cttgaagtcg ccctggctgt
     3001 agtggacctg gccgatcgcg ttgagggttc cggcgacccg cgggtgctcg ctgccggcgg
     3061 tcgcccgcac gccgtcgagg ttctgctgga acgccgcgag ggcgccggcc gggtcgccga
     3121 ggcgatgcag cgcgcgaccg aggtcgagca agacggcgag cgtgtcctcg tgcccgccgc
     3181 cgaggctgcg cgtcagcagc tcgagcgcgc ggcggtaggc gctgacggag tcgtcgtact
     3241 ggccctgctg gtagaggat
//
LOCUS       DASWNJ010000646         1944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_224066, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000646 DASWNJ010000000
VERSION     DASWNJ010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224066"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..629
                     /locus_tag="VGB85_10860"
     CDS             141..629
                     /locus_tag="VGB85_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134576.1"
                     /translation="MRCLPALIITALLVQIPAATAMALDLDRLREILKRIVDFITLGG
                     VALALLAGLVFLVTWVLSHLRRPSDRSHRYLSATAMTTAWLPVLLTTVAAAGMFVPTL
                     APFVLRAFALAVALAAVAWCVSVTSLIAGGTRRDLRRTRKALILAGTPWYCLVVYLAT
                     YL"
     gene            complement(646..1065)
                     /locus_tag="VGB85_10865"
     CDS             complement(646..1065)
                     /locus_tag="VGB85_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134577.1"
                     /translation="MSRRSRMGKLLLLASLLAGCRHGGPNPRTLHYVGKDLVATRSAD
                     PRAYAHYVQCQLALSLDPPDLAHAHEQILLAIDHDPRDAHLWTVRGQIELQRGDQAQA
                     RRASERALYLRPDYPPAKELAARIAGQDGPAVAQRRP"
     gene            complement(1062..>1944)
                     /locus_tag="VGB85_10870"
     CDS             complement(1062..>1944)
                     /locus_tag="VGB85_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY0134578.1"
                     /translation="GVAHFSGASEPAVIAMTRELLGFFPSNNMEEAPRRPTADEPGRM
                     CPELADFVPVDASKPYDIRTIITAVADDGHFVEVQEHFARNIVIGFIRIDGESVGVVA
                     NQPLVLAGCLDINASVKAARFVRMCDAFNIPLWTLVDVPGFLPGTEQEFGGIIRHGAK
                     LLYAFVEATVPKVTLVTRKAYGGAYDVMASKHIRADINLAYPTAEIAVMGAEGAVNII
                     YRRELADAEDPAAMRAELLANYRGLFANPYKAAELGFIDQVIAPEETRRHVARSLKLL
                     RNKRQENPRKKHGNIPL"
BASE COUNT          248 a          689 c          690 g          317 t
ORIGIN      
        1 tgagctacac ccgcgtgggt cgaagggtgt acctcagggc gaacccgctt ggcaagcgca
       61 gcggcgcgag aggtgcgccc acgcgcgacc gcccggccgg gggcattgcg cgccgcaagc
      121 cggtgttata ctggccgacg atgcgctgtc tgcccgctct catcatcacg gccctgctgg
      181 tccagatccc ggcggcgacc gccatggccc tcgacctcga ccggctccgg gagatcctca
      241 agcggatcgt cgactttatc accctcggcg gcgtcgccct cgcgctgctc gccgggctgg
      301 tgttcctggt gacctgggtg ctctcgcacc tgcgccgccc gagcgaccgg tcacaccgct
      361 acctctccgc gaccgcgatg acgaccgcct ggctgcccgt gctgctgacc accgtcgccg
      421 cggccggcat gttcgtgccg accctggcgc ccttcgtgct ccgcgccttc gcgctggccg
      481 tcgcgctcgc cgcggtcgcc tggtgcgtct cggtcacctc gctgatcgcc ggcggcaccc
      541 gccgcgacct gcgtcgcacc cgcaaggcgc tgatcctcgc gggcacgccc tggtactgcc
      601 tcgtcgtcta cctggcgacc tacctctgag ccccgtcgtt cttgctcatg gccggcgctg
      661 cgcgaccgcg gggccgtcct gaccggcgat ccgcgcggcc agttccttgg ctggcgggta
      721 gtccgggcgc aggtacagcg cccgctcgct ggcccgccgc gcctgggcct ggtcgccgcg
      781 ctgcagctcg atctggccgc gaacggtcca caggtgggcg tcgcgcgggt catggtcgat
      841 cgccagcaag atctgctcgt gcgcgtgggc caggtccggc gggtcgagcg acagcgccag
      901 ctggcactgc acgtagtgcg cgtaggctcg cgggtccgcc gagcgcgtcg ccaccaggtc
      961 cttgccgacg tagtgcagcg tccgcgggtt cggaccgccg tggcgacagc ccgccagcag
     1021 cgacgcgagc agcagcagct tgcccatgcg agaccgacgg ctcacagcgg gatattgcca
     1081 tgcttcttgc gcgggttctc ctgtcgcttg ttgcgcagca gcttgaggct gcgcgcgacg
     1141 tggcggcggg tctcctccgg cgcgatcacc tggtcgataa agccgagctc ggcggccttg
     1201 tacgggttgg cgaacagacc ccggtagttg gcgagcagct ccgcccgcat cgcggccggg
     1261 tcctcggcgt cggcgagctc gcggcgatag atgatgttga ccgccccctc cgcgcccatc
     1321 actgcgatct ccgcggtcgg gtacgcgagg ttgatgtcgg cgcggatgtg cttgctcgcc
     1381 atgacgtcgt aggcgccccc gtaggccttg cgggtcacca gcgtcacctt cggcaccgtc
     1441 gcctcgacga aggcgtacag cagcttggcg ccgtggcgga tgatgccgcc gaactcctgc
     1501 tcggtgcccg gcaagaagcc cggcacgtcg acgagggtcc acagagggat gttgaacgcg
     1561 tcgcacatgc gcacgaagcg ggcggccttg acggaggcgt tgatgtcgag gcagccggcg
     1621 agcacgagcg gctggttggc gacgacgccg accgactcgc cgtcgatgcg gatgaagccg
     1681 atgacgatgt tgcgggcgaa gtgctcctgg acctcgacga agtggccgtc gtcggcgacc
     1741 gcggtgatga tcgtgcggat gtcgtagggc ttgctggcgt cgaccggcac gaagtcggcg
     1801 agctccgggc acatacggcc gggctcgtcg gcggtcgggc ggcgcggcgc ctcctccatg
     1861 ttgttcgacg ggaagaagcc gagcagctcg cgcgtcatgg cgatcaccgc cggctccgag
     1921 gcgccagaga agtgggcgac gccg
//
LOCUS       DASWNJ010000647         2084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_364163, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000647 DASWNJ010000000
VERSION     DASWNJ010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364163"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="VGB85_10875"
     CDS             <1..903
                     /locus_tag="VGB85_10875"
                     /inference="COORDINATES: protein motif:HMM:NF020032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134579.1"
                     /translation="DDAHAAAIAGGLAARGVRAEHLDFADFPAAAELSWDPLARRGVL
                     ARASGPALALESVRSVYWRTATRTRAATGLRRDLHGFAEDESRRALECILRALPCPVI
                     NTPAAVTAHRLKPLQSAQVAALGIPTPATRISSSPAEVRSFFAAHPQGVVVKPIGGGA
                     YVRKLVPADLEREHGIRACPMQYQAYIPGEDLRVYVIGTRVFAARIVTRDPDHVDFRT
                     DPTHHSVACELDRDSALRSLRIARALDLAFTGIDFRRTPAGELVFLEANPSPMFLRFA
                     ADTGHPLAQALIDLLLVPGAERAA"
     gene            936..1988
                     /locus_tag="VGB85_10880"
     CDS             936..1988
                     /locus_tag="VGB85_10880"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY0134580.1"
                     /translation="MRRVLHVIVTTGLFSGACGGARDTDDIGFKVTGFEVRDIEIPGR
                     LHLEGLTVPAKLFVPILEEGARPMPAVVVLHGSGGLFAMPDETVVGRPCSPDLEPQFA
                     RWGERLARLGYVAIMPASHDARGFCDYYDDRDRIPDDFDDPQERLLGRLYDTDAASRH
                     LCDMPEVDCDRLGLLGFSHGASTAMLALHWQLRRALGELGDSLDLDLPVAPLPPGAPS
                     FQVGIAYYPGCGLESVVHMGSDADDDPMDMYFPDADLYVEHGSDDDLINDCSVDFGEG
                     RRQLQSATVARVDDLPDPYHIRVHRDARHGFDNAGSEGTDEGSGGNRPADIRARDAAL
                     TATLQHLSDYLGGARR"
BASE COUNT          277 a          847 c          650 g          310 t
ORIGIN      
        1 gacgacgcgc acgccgcagc gatcgccggt gggctcgcgg cgcgcggggt ccgggcggag
       61 cacctcgact tcgccgactt ccccgccgcc gccgagctga gctgggaccc actcgcgcgt
      121 cgcggggtgc tcgcgcgcgc cagcgggccc gcgctcgcgc tcgagtccgt acgctcggtc
      181 tactggcgca ccgccacgcg cacgcgcgcc gcgaccggcc tgcgtcgcga tctccacggc
      241 ttcgccgagg acgagtcacg gcgcgcgctc gagtgcatcc tgcgcgccct gccctgcccc
      301 gtcatcaaca cccccgccgc cgtcactgcg catcgcctca agcccttgca gtcggcccag
      361 gtcgccgcgc tcggcatccc gacaccggcc acgcggatct ccagctcgcc cgccgaggtg
      421 cgcagcttct tcgccgcgca tccgcagggc gtcgtcgtca agccgatcgg cggcggcgcc
      481 tatgtccgca agctcgtccc cgcggatctc gagcgcgagc acggcatccg cgcgtgtccg
      541 atgcagtatc aggcctacat ccccggcgag gatctccgcg tgtacgtgat cggtacccgg
      601 gtgttcgccg cgcgcatcgt cacccgcgat cccgaccatg tcgactttcg caccgacccc
      661 acgcatcaca gcgtcgcctg cgagctcgat cgtgacagtg ccctgcgcag cctgcggatc
      721 gcccgggccc tcgacctcgc gttcaccggc atcgacttcc ggcgcacgcc ggccggtgag
      781 ctggtgttcc tcgaggccaa tcccagcccg atgttcctgc gtttcgcggc agataccggc
      841 cacccgctcg cgcaggcgct gatcgacctc ctgcttgtgc ccggtgccga gcgcgcagct
      901 tgacgctccc ccccgaccct ccgtacgctg tcgatgtgcg cagggtcctt cacgtcatcg
      961 tcactaccgg tctgttctcc ggtgcgtgcg gcggcgcgag agacacggac gacatcggct
     1021 tcaaggtcac cggcttcgag gttcgcgata tcgagatccc cggacgcctg cacctcgagg
     1081 gcctgaccgt ccccgccaag ctgttcgtcc cgatcctcga ggagggcgcg cgaccgatgc
     1141 ccgccgtcgt cgtgctgcac ggctccggtg ggctgttcgc gatgcccgac gagaccgtcg
     1201 tcggtcgccc gtgcagcccc gatctcgagc cgcagttcgc ccgctggggc gagcgcctcg
     1261 cgcggctcgg ctacgtcgcg atcatgcccg ccagccacga cgcccgcggc ttctgcgact
     1321 actacgacga ccgcgaccgc atccccgacg acttcgacga cccgcaggag cgcctgctcg
     1381 gccgcctcta cgacaccgac gccgccagtc gccacctgtg cgacatgccc gaggtcgact
     1441 gcgaccgcct cggcctgctc ggcttctcgc acggcgcctc gaccgcgatg ctcgcgctgc
     1501 actggcagct gcgccgcgct ctcggtgagc tcggcgacag cctcgacctc gacctcccgg
     1561 tcgcgccgct gcccccgggc gcgccgagct tccaggtcgg catcgcgtac taccccggct
     1621 gcggcctcga gagcgtcgtc cacatgggct cggacgccga cgacgacccg atggacatgt
     1681 acttcccgga cgccgacctc tacgtcgagc acggcagcga cgacgacctg atcaacgact
     1741 gcagcgtcga cttcggcgag ggccgccgcc agctccagtc ggccaccgtc gcgcgcgtcg
     1801 acgacctgcc ggacccctat cacatccgcg tgcatcgcga cgcccgccac ggcttcgaca
     1861 acgccggcag cgagggcacc gacgagggca gcggcggcaa ccgaccggcc gacatccggg
     1921 cgcgcgacgc cgccctcacg gccacgctgc agcacctctc ggattacctc ggcggcgctc
     1981 gccgctgacc gtccgcaagt tctttcgctc gcgccggaac ctccgcgaac cgtcgctgtc
     2041 tcgctcgacc acggagccag gcatgcaacg acgcaagttt ctcg
//
LOCUS       DASWNJ010000648         2387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_542286, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000648 DASWNJ010000000
VERSION     DASWNJ010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542286"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VGB85_10885"
     CDS             <1..383
                     /locus_tag="VGB85_10885"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEY0134581.1"
                     /translation="AAEAEGKFWEFHDRLYATRAQFDEASLLTIARDIGLNAKKFKQR
                     LHSDEFDAKIIADQELGHTLGIRGTPAYFVNGRALDGAVPELEFRLTIQEELERAEDL
                     LREGVPAAGLYERLLTPKRPDASR"
     gene            508..1641
                     /locus_tag="VGB85_10890"
     CDS             508..1641
                     /locus_tag="VGB85_10890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134582.1"
                     /translation="MILSIVGCGGNVVEGTTTDTGSSSTSTMATTGELPTTGDTTTTD
                     PTSGTTEGVSVGDTTTVDTTGETTTGDTTGDTTGDTTTGDTTTGDTTTGDTTGTGDTT
                     AGDSTAGESTGTGGSTDTGSESSSTGEPLVCGDGQPGPGELCDDGNADDSDECTSECL
                     MAMCGDGFVQTDVDACDDANAVNNDACSNKCVLTPTAVTLQMGANTPQAGNVNGGVAY
                     NDPCPAGKLLIGFQGFVGTYHGRIQGVCGSPGLVPAMEGFAVAVTEPVLLPQRGLQGN
                     MQWMRMCPIDQAIVGFSGRSGGLVDQLTFTCAPLIVSEAIDGTWSIATGPTNNLPPIG
                     GNGGQPFAQASCPMGQVGAMQRVRAGDGIDAFGLGCSTVGLAF"
     gene            1906..>2387
                     /locus_tag="VGB85_10895"
     CDS             1906..>2387
                     /locus_tag="VGB85_10895"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0134583.1"
                     /translation="MPDRPAAQIPGLQVLGELGRGARSIAYRARRGDFVVALKLPVAG
                     RSDPGAARQFRREAVALASVAHPCLPQVFEVGEVDGLPYVIRELAEGRSLASELAAGP
                     LPEAEVAALGRQLAQALAAIHRRRLVHREVAPHNVLRIDADLVKLIDFGTPRGGEGSQ
                     A"
BASE COUNT          357 a          804 c          909 g          317 t
ORIGIN      
        1 tggcggccga ggccgagggc aagttctggg agttccacga ccggctgtac gcgacgaggg
       61 cccagttcga cgaggcctcg ctgctgacga tcgccaggga catcgggctc aacgcgaaga
      121 agttcaagca gcgcctgcac agcgacgagt tcgacgcgaa gatcatcgcc gaccaggagc
      181 tcgggcacac gctggggatc cgcgggacgc cggcctactt cgtcaacggg cgcgcgctcg
      241 acggggcggt gcccgagctc gagttccgcc tgacgatcca ggaggagctc gagcgcgccg
      301 aggacctgct gcgcgagggc gtgccggcgg cggggctgta cgagcggctg ctgacgccga
      361 agcggcccga cgcgtcgcgc tgaggcgcgc gccgcgaggg tgcgccgtcc ggtgttagca
      421 tggcgcatgc ttgccagcct gcccagttca tcgccctgtc gctcgacccc caccgtatcc
      481 accggcgtac atgcgagcgc cgccctgatg atcctgtcga tcgtcggctg cggcggaaac
      541 gtggtcgagg gcaccaccac ggacacgggc tcctcgtcga cctcgacgat ggccacgacc
      601 ggcgagctgc cgaccaccgg tgacacgacg acgacggatc cgacgagcgg cacgacggag
      661 ggcgtgtccg tcggcgatac gacgacggtc gacacgacag gcgagacgac gacaggcgac
      721 acgacgggcg acacgacggg cgacacgacg acgggcgata cgacgacggg cgacacgacg
      781 accggtgaca cgacgggcac cggcgatacg accgctggcg attcgacggc cggcgagtcg
      841 accggcacgg gcggctcgac cgataccggg tcggagagca gctccactgg cgagccgctg
      901 gtctgcggcg acggccagcc cggccccggc gagctgtgtg acgacggcaa cgccgacgac
      961 agcgacgagt gcaccagcga gtgtctgatg gcgatgtgcg gtgatggttt cgttcagacc
     1021 gacgtcgacg cctgcgacga cgcgaacgcg gtcaacaacg acgcgtgcag caacaagtgt
     1081 gtgctgacgc cgacggcggt gaccttgcag atgggggcca acaccccgca ggccggcaac
     1141 gtcaacggcg gcgtggccta caacgacccg tgcccggccg gcaagctgct gatcggcttc
     1201 caggggtttg tcggcaccta ccacgggcgg atccagggcg tgtgcgggtc acccgggctg
     1261 gtcccggcga tggagggctt cgcggtcgcc gtcaccgagc cggtgctgct gccccagcgc
     1321 ggccttcagg gcaatatgca gtggatgcgg atgtgcccga tcgaccaggc gatcgtcggc
     1381 ttcagcgggc gctcgggcgg gctcgtcgat cagctgacgt tcacctgcgc cccgctgatc
     1441 gtcagcgagg cgatcgacgg cacgtggtcg atcgccaccg gcccgacgaa caacctcccg
     1501 ccgatcggtg gcaacggcgg gcagccgttc gcgcaggcga gctgtcccat gggtcaggtc
     1561 ggcgcgatgc agagggtccg cgcgggtgat ggcatcgacg cgttcggcct gggctgcagc
     1621 accgtcggcc tggcgttcta gcgatgtgca gaagagtggc cggtgaccag gtcgctcgcg
     1681 ccgacccggc cgcgagctcg atgcgatctt gctcgccacc tcgtgacgag gccccggtgt
     1741 cgcgtcagcg cgcgggcccg ggaggggcgg actgtggtcg ggcgcacagg gcgggcgccc
     1801 gtggctgctc tcgcgggcac ccacgggcgt gtgcgcgcag gtgcgcggct tcgcgcgtgt
     1861 cgcctgcggc ggcgatgacg agaaaaccag cgatactcgg gcgccgtgcc cgaccgcccc
     1921 gccgcgcaga tccccggctt gcaggtgctc ggcgagctgg gtcgtggggc tcgcagcatc
     1981 gcctatcggg cgcgccgcgg tgacttcgtg gtggcgctca agctcccggt cgcgggtcgc
     2041 agcgaccccg gcgcggcgcg gcagtttcgc cgcgaggcgg tcgcgctggc cagcgtggcg
     2101 cacccgtgtc tgccccaggt cttcgaggtc ggcgaggtcg acggtctgcc gtatgtgatc
     2161 cgcgagctgg cggaggggcg cagcctggcg agcgagctgg cggcggggcc gctgccggag
     2221 gccgaggtcg cggcgctcgg tcgccagctc gcgcaggcgc tggcggcgat ccaccggcgg
     2281 cggctcgttc atcgcgaggt ggcgccgcac aacgtgctgc ggatcgacgc cgacctggtc
     2341 aagctgatcg acttcggtac gccacgcggc ggcgagggct cgcaggc
//
LOCUS       DASWNJ010000649         2084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_188837, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000649 DASWNJ010000000
VERSION     DASWNJ010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188837"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VGB85_10900"
     CDS             <1..387
                     /locus_tag="VGB85_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134584.1"
                     /translation="RGTTMSLRSRVLRGVALAAVVLVPAVAYADVDGTKGTVVSVTIN
                     DTAADDYNAERGNIVLNEGSATRKYQWGGTVCSAKNLSDANIALLIDALSAKDRLEIV
                     PSYKTGAGQARCLVGFRIQLIQSEPR"
     gene            497..1081
                     /locus_tag="VGB85_10905"
     CDS             497..1081
                     /locus_tag="VGB85_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134585.1"
                     /translation="MADDGSIAPDPRRLARAQRAGLRPGSPWLTPAVMCLALAAGLRW
                     GGPAALGRWRAVWMQGTVEGVGAVVVPLMIGAAVVAVAVAALAGSLGWVDARAREGLA
                     VGELRSAGRGLLALLVPVLVVLVLAGVVAGAARAVDASEAALIGLWWAWAQRLLVGVG
                     GLLLVAGVVDRALGRRRLWLALHRDPDELRRGEG"
     gene            1078..>2084
                     /locus_tag="VGB85_10910"
     CDS             1078..>2084
                     /locus_tag="VGB85_10910"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134586.1"
                     /translation="MSERLISVALPVSIDRAFTYAVPPDWPSVPEPGARVLVPFASRG
                     LVGVVREAPGEPTAKVRRVEETLDPPGAPSLTPGLLRLCEWVQDYYVAPVGEVYRLAL
                     PGLLTGSDVRKARLTAAGEQALASSRDFVLTSQGGGPYIPSVAEQRVLAALATAPKHE
                     LAVDRLTSLKPRISGVLRILGELAARDLCTIHWDDGGDEGARVETHYRRTDYLRGGGG
                     NEEAIQTIVGRSKQKRALLDFMDTAPQGVWIPAGEVRGPFPRVRELMPGLLEAGLVVA
                     EERLRALDPFAAGAPESSTPQAPTEDQRRSLAALITDMDAGAFAVSLLHGVTGSGKTE
                     V"
BASE COUNT          247 a          663 c          861 g          313 t
ORIGIN      
        1 agaggaacaa caatgtcgct tcgttcacga gttctcaggg gggtcgcgct ggcggccgtg
       61 gtgttggtgc cggccgtcgc gtacgccgat gtcgatggga ccaagggtac ggtcgtctcg
      121 gtgacgatca acgacaccgc cgccgacgat tacaacgccg agcgcggcaa catcgtcctc
      181 aacgagggat ccgcgacccg caagtaccag tggggcggga cggtgtgtag cgccaagaac
      241 ctgtccgacg cgaacatcgc cctgttgatc gacgcgctga gcgccaagga ccgcctggag
      301 atcgtgccga gctacaagac cggcgcgggc caggcgcgct gtctcgtcgg ctttcgcatc
      361 cagctgatcc agtccgagcc gcgctgagcc cggcccggtg atgtgcgcga gctggcccgg
      421 gtgcggccgg ctcgccgcgc ttggggggtg gaagatccct tggccgtcgg gcgcgggcgc
      481 gatacacctc gctgccttgg cggacgacgg cagcatcgca cccgacccgc ggcgcctcgc
      541 ccgggcgcag cgcgccggac ttcgcccggg ctcgccgtgg ctgacgccgg ctgtgatgtg
      601 cctggccctc gccgccggcc tgcgctgggg cggacccgcg gcgctggggc gctggcgagc
      661 agtgtggatg caagggaccg tcgagggggt cggcgccgtg gtggttcccc tgatgatcgg
      721 ggcggcggtg gtcgcggtcg cggtcgcggc gctcgccggc tcgctcgggt gggtcgacgc
      781 gcgggcgcgt gaggggctcg cggtcggcga gctgcgcagc gccgggcgcg ggctgctcgc
      841 gttgctcgtg ccggtgctgg tggtgctcgt gctcgcgggg gtggtcgcag gcgcggcgcg
      901 ggcggtcgat gccagcgagg ccgcgctgat tggtttgtgg tgggcgtggg cgcagcggct
      961 gctcgtcggg gtcggcgggc tgttgttggt cgccggcgtc gtcgacaggg cgctcgggcg
     1021 gcggcggctg tggctcgcgc tgcatcgtga tccggacgag ctgcggcgag gcgaagggtg
     1081 agcgagcgac tgatctcggt ggcgctgccg gtcagcatcg accgcgcctt cacctacgcg
     1141 gtgccgcccg actggcccag cgtgcccgag cccggcgcgc gcgtgctggt gccgttcgcc
     1201 tcgcgcgggc tggtcggcgt cgtgcgcgag gcgcccggcg agccgacggc caaggtgcgg
     1261 cgcgtcgagg agacgctcga cccacccggc gcgccgtcac tgacgccggg gctgctgcgt
     1321 ctgtgcgagt gggtgcagga ctattatgtc gcgccggtcg gtgaggtcta tcgcctggcg
     1381 ctgccggggc tgctgacggg atcggatgtc cgaaaggcca ggttgacggc ggcgggcgag
     1441 caggcgctgg cgtcgtcgcg ggacttcgtg ctgacctcgc agggcggcgg gccgtacatc
     1501 ccgtcggtgg ccgagcagcg ggtgctcgcg gcgctggcga ccgcgccgaa gcacgagctc
     1561 gcggtcgatc gcctgacctc gctcaagccc aggatcagtg gcgtgctgcg gatcttgggt
     1621 gagctggcgg cgcgcgacct gtgcacgatc cactgggacg acggcggcga cgagggggcg
     1681 cgggtcgaga cgcactatcg gcgcaccgat tacctgcgcg ggggcggtgg gaacgaggag
     1741 gcgatccaga cgatcgtcgg ccgtagtaaa cagaagcggg cgctgctcga cttcatggac
     1801 acggcgccgc aaggggtgtg gatcccggcg ggcgaggtgc gcgggccgtt ccctcgcgtg
     1861 cgtgagctga tgccggggct gctcgaggcg gggctggtgg tcgcggagga gcggctgcgg
     1921 gccctcgatc ccttcgcggc aggggccccg gagagctcga cgccgcaggc gccgacggag
     1981 gaccagcgac gctcgctcgc ggcgttgatc accgacatgg acgcgggggc cttcgcggtg
     2041 tcgctgctgc acggggtcac cggctcgggc aagacggagg tgta
//
LOCUS       DASWNJ010000650         1619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_260146, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000650 DASWNJ010000000
VERSION     DASWNJ010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260146"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(399..>1619)
                     /locus_tag="VGB85_10915"
     CDS             complement(399..>1619)
                     /locus_tag="VGB85_10915"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY0134587.1"
                     /translation="VSAQSVVSHGRRFGDRDLTAVGLAALGRLTLYAGRVPDGLALFD
                     EAMASVTAGEVTSPIFAGNVYCVMIEGCQEVSDLGRAAAWTAALTRRCSEQPGLKAFT
                     GQCAVHRGQIMRLHGAFAAAIAEFDAAVRRYLASPTTAPAGVALAERGDVHRLLGDLD
                     AAEASYEQATALGYEPQPGLALLWLARGRTPAALAAVRRLLAEIPDPVRRSRILPAAV
                     DILIAGEDLAGAHVAAAELDAIARDFGCDALKAVAAYASGRLELECGDASGALPYLRK
                     ASTLWAAMAAPYDAARARVLIAHALGALGDAESATRELDGAHRTFVELGAVPAADEIT
                     RHQAPVTLPDGLTDREAEVVRLVATGRSNAQIAADLVLSEKTVARHLSNIFTKIGVGS
                     RTAAAAYAFEHGLA"
BASE COUNT          233 a          557 c          597 g          232 t
ORIGIN      
        1 gaggcacatt tgttcgagga cgagggtcac ctctcgctct tcgcgaagct ggaggtgatc
       61 ctggctgacc tcaagcggct gggtggggtc tgaggcgcac cctggtgtct cctgagcccg
      121 acgtggccag ggggcctcgg ccgccccgcg tcgtaccggc ttgaggcggt tggcttcagc
      181 cctcgagggc tcggcgggtc cgcgcatgtg accagacgcc gcggacacgt cggcgaggcg
      241 tccgagtcct gcgcagctca gagtcgcgct cgcgcgcgct catcaagatg gtggcggggt
      301 tggcggccat agggttcaca acgagctccc aggtgagtag gtctttccca gactggccct
      361 aggacagcct tttgccatcc ggggatctac ctatctcgtc aggccaggcc gtgctcgaag
      421 gcgtacgccg ccgcggcggt gcgcgagccg acgccgatct tcgtgaagat gttgctgagg
      481 tggcgcgcga cggtcttctc gctgaggacg aggtcggcgg cgatctgggc gttgctccgg
      541 ccggtggcaa ccaggcgcac gacctcagcc tcgcgatcgg tcagcccgtc ggggagcgtg
      601 accggagcct ggtgccgggt gatctcgtcg gcggccggga cggccccgag ctccacgaag
      661 gtgcgatgtg caccgtcgag ctcccgggtg gccgactccg catccccaag ggcacccaga
      721 gcgtgggcga tcaggacgcg tgctcgtgcc gcgtcgtagg gagcagccat ggccgcccag
      781 agggtcgacg ccttcctcag gtaggggagt gcgccggacg cgtcgccgca ctcgagctcg
      841 agccgcccgg aggcgtacgc cgcgaccgcc ttgagggcgt cgcacccgaa gtcgcgcgcg
      901 atcgcgtcga gctcggccgc ggcgacgtgg gcacccgcca ggtcctcccc ggcgatcagg
      961 atgtcgacgg cggccggcag gatgcgagat cgccgcaccg gatcggggat ctcggcgagc
     1021 agccgccgca ctgccgccag ggccgctggg gtacgtccgc gcgcgagcca cagcagggcg
     1081 agccccggct gtggttcgta gccgagagcg gtggcctgct cgtagctcgc ctcggccgcg
     1141 tccaagtccc cgaggaggcg atggacgtcc ccgcgctccg cgagggcgac tcccgcgggc
     1201 gccgtcgtgg gtgaggcgag atagcgacgc accgctgcgt cgaactccgc gatcgccgcg
     1261 gcgaacgccc cgtggagccg catgatctga ccgcggtgca cggcgcactg gccggtgaac
     1321 gctttgagtc cgggctgctc actgcacctg cgcgtgagcg cggccgtcca cgcggccgca
     1381 cggccgagat cggagacctc ctggcagccc tcgatcatga cgcagtagac gttgccggcg
     1441 aagatcggcg acgtcacctc gccggcggtc acggacgcca tcgcctcgtc gaagagcgcc
     1501 aacccgtccg gcacacggcc ggcgtacaac gtcaggcgcc cgagtgcggc caggcccacc
     1561 gcggtgagat ctcgatcacc gaagcgacgg ccgtggctga ccacggactg ggcggaaac
//
LOCUS       DASWNJ010000651         1690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_505624, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000651 DASWNJ010000000
VERSION     DASWNJ010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505624"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VGB85_10920"
     CDS             complement(<1..780)
                     /locus_tag="VGB85_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134588.1"
                     /translation="MTLRRGDLAVTRSLTASSCAALAEATAVIAATACPALDDADADV
                     ASEPVVEPPPAAAEVLVPEVEVEVPDEPAPAVAPIPAPPQPDEPAPAVTPGRKRAGLE
                     LGARLASAWGPTPAPALVAGASLRVQWPAFELLFALDGGPRRRVRGAAGPGIDMRSAT
                     ATIAGCPTAVFGSGRRRTRLSLCAGLEAGVLVGTGVGTPVTRTAAQPWLAALVGPSLT
                     WSPRGRLNLGAAVDLAASLTRPAFSLAGYDTNFRAAATSVRL"
     gene            1263..>1690
                     /locus_tag="VGB85_10925"
     CDS             1263..>1690
                     /locus_tag="VGB85_10925"
                     /inference="COORDINATES: protein motif:HMM:NF017739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HEY0134589.1"
                     /translation="MAAPAKNRACFLFEIDGIADELRVVSFDLVESISDLFELNVELA
                     CSNRDLDVASILRKPALLTILGVHEDDGPRWVHGFVHSTSQGRQDDLLALFHVRVVPQ
                     VWPLLHRQDCRIFQDMSTTEIAEDVLKRAGIAKSGFRFALA"
BASE COUNT          246 a          613 c          601 g          230 t
ORIGIN      
        1 caagcgcacg ctcgtcgcgg ccgcgcgaaa gttggtgtcg tagcccgcca gcgagaacgc
       61 cgggcgcgtg aggctcgccg cgaggtcgac cgcggcgccg aggttgaggc ggccgcgcgg
      121 cgaccaggtc aggctcggcc cgaccagcgc ggccagccac ggctgcgccg ccgtgcgcgt
      181 gaccggcgtg ccgacgccgg tgccgacgag cacgcccgcc tcgaggccgg cgcacaggga
      241 cagccgggtc cgtcggcgcc ccgacccgaa cacggccgtc gggcagccgg cgatcgtggc
      301 cgtggcggag cgcatgtcga tgcccggccc ggcggcgccg cggacccgtc ggcgcggccc
      361 accgtcgagc gcgaacaaca gctcgaacgc gggccactgc acccgcaacg aagcgccggc
      421 gaccagcgcc ggggcagggg tcggccccca cgcgctcgcc aggcgggcgc ccagctcgag
      481 gcccgcccgc ttgcggcccg gggtgacggc cggcgcgggc tcgtcaggct gcggcggcgc
      541 gggtatcggg gcgaccgcag gggccggctc gtccgggacc tcgacctcga cttcgggcac
      601 caggacctcg gccgcggcgg gcggaggctc cacgaccggc tcactcgcca cgtccgcgtc
      661 cgcatcatcc agcgcggggc acgcggtcgc cgcgatcacg gccgtcgcct cggccagcgc
      721 cgcgcagctg ctcgccgtca ggctgcgcgt gaccgccagg tcgccccggc gcagcgtcaa
      781 ttccatgtcc cagcggcgat tcgcccgcgt tgcgaccgcc gtgacccgca acggctcggt
      841 gccgccctgc tggcaccgct gcggggcgag ggcacgaaaa cggcgctcga cggcggcccc
      901 atcggggcat tccgctgggg cttgccaccc ccattcgacc ggacgagggt cgggctccgc
      961 ggcgctcagg aggaggaaaa gtagcaaagg atgcctcgga cgggtcctga acatagcagc
     1021 catccgaaaa atgcgccccc cgtcgacgac gcctcgtcga cgcgcccgcg gtgaccgtcg
     1081 gcggcggctt gacagcgcgc ggtcatgcag ccgtccgacg cgctgctccg tggcgacatc
     1141 gatcgcccgt caccgatgca tcgatcgtgc gaatatgtcc atatcaatgg gcggtttcgg
     1201 caatacggcc gctcgcattg ccatggtttt acacgctcga tacactcctg ctctggggtt
     1261 ttatggcggc gccagcgaag aatcgtgcgt gttttctatt cgagattgac ggcatcgcgg
     1321 acgagctccg cgtcgtcagc ttcgacctcg tcgagtcgat atccgatctg ttcgagctca
     1381 acgtcgaatt ggcgtgctcc aaccgggacc tcgacgtggc gagcatactc cgcaagcccg
     1441 cgctgctgac gatcctcggt gttcacgagg acgacgggcc gcgatgggtc cacgggttcg
     1501 tccacagcac cagccagggc cgccaggacg acctgctcgc gctgttccac gtgcgcgtgg
     1561 tcccgcaggt gtggccgctc ctgcaccgcc aggattgccg catctttcag gacatgagca
     1621 cgacggagat cgcggaggac gtgctcaagc gggcggggat cgcaaagagc gggtttcgct
     1681 tcgcgctggc
//
LOCUS       DASWNJ010000652         1504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_47457, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000652 DASWNJ010000000
VERSION     DASWNJ010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47457"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(274..1026)
                     /locus_tag="VGB85_10930"
     CDS             complement(274..1026)
                     /locus_tag="VGB85_10930"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY0134590.1"
                     /translation="MLTRLEWLAAAPYTLALGAGFFGFFAHTGVLLALEQSGAARPRR
                     IVGVSAGALAGGLWASGLTAREIETSLVELRRIDFWDPGLPIGGLLRGDKFAGRLHTL
                     LDARGVARIEDCPTAFATVVYELRTRRTRVLDRGRIAPAIQASCTVPLMFRPLRHEGR
                     LLVDGGVADRDGETALAPDERVMQHRLLSRSPWSAITRRTQAISKVSERTGRQVLVLD
                     TLPRVSPFRLEFGPHALATAREATLRWLEERV"
     gene            complement(1047..>1504)
                     /locus_tag="VGB85_10935"
     CDS             complement(1047..>1504)
                     /locus_tag="VGB85_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134591.1"
                     /translation="EAGEGGLIADILSRRPDQDGPRLGTKLAASLQRHPLQRLPPPGP
                     QRFESMLRQALARPLAIGTAMLSWQVASLERAADAAVGWSLVLGKRNEHVQRALAEAP
                     GPEDAEVATALAIAALFSAARKTHGRDSAVVDEWIAEYRLLALVYAHAP"
BASE COUNT          230 a          631 c          464 g          179 t
ORIGIN      
        1 gcgcagctcc agcgcggcgc gcccgagccc gagcccgagc ccgacccctg atcctcaaac
       61 gcgttcctca agccagcaca gctccagctc agcgcacccg agcccgagcc cgagcccccc
      121 tgaccttcaa acgcgttcct caagccagcg cagctccagc gcggcgcgcc cgagcccgag
      181 cccgagcccg acccctgatc ctcaaacgcg ttcctcaagc cagcgcagct ccagctcagc
      241 gcacccgagc ccgagcccga gcccccctga ccttcaaacg cgttcctcaa gccagcgcag
      301 cgtcgcctcg cgcgcggtcg ccagcgcgtg cggcccgaac tcgaggcgaa acggcgacac
      361 ccgcggcagc gtatcgagca ccagcacctg ccgacctgtc ctctcggaca cctttgaaat
      421 cgcctgcgtc cgccgcgtga tcgcgctcca cgggctgcgc gacagcagtc ggtgctgcat
      481 cacgcgctca tccggcgcga gcgccgtctc gccgtcgcgg tccgcgaccc cgccgtcgac
      541 cagcagccgc ccctcgtgcc gcagcgggcg gaacatcagc ggcaccgtgc acgacgcctg
      601 gatcgccggc gcgatccgcc cgcgatcgag cacccgtgtc cgtcgcgtcc gcaattcata
      661 aacaaccgtg gcgaacgccg tcggacagtc ctcgatccgc gccaccccgc gcgcgtcgag
      721 cagcgtgtgc aagcgccccg cgaatttatc cccgcgcagc agcccgccga tcggcagccc
      781 gggatcccag aagtcgatcc tgcgcagctc gaccagcgat gtctcgatct cccgcgccgt
      841 cagcccgctc gcccacagcc caccggccag cgcccccgcg ctgaccccga cgatccgccg
      901 cggccgcgcg gcccccgact gctcgagcgc cagcagcacg ccggtgtggg cgaagaagcc
      961 gaagaaacct gcgccgagcg ccagggtgta cggcgccgcg gcgagccatt cgagacgggt
     1021 cagcattgcg gcgcgcagtg tacacgtcac ggcgcgtgcg catagaccag cgcgagcagg
     1081 cgatactccg cgatccactc gtcgaccacg gcgctgtcgc ggccgtgggt cttgcgcgcc
     1141 gccgagaaca gcgccgcgat cgccagcgcc gtcgcaacct ccgcgtcttc cgggcctggc
     1201 gcctccgcca gcgcccgctg cacgtgctcg ttgcgcttgc cgagcaccag gctccacccg
     1261 accgccgcgt ccgcggcccg ctcgaggctc gccacttgcc aggagagcat cgccgtcccg
     1321 atcgccagcg gtcgcgccag cgcctggcgc agcatcgact cgaagcgctg cggacccggc
     1381 ggcggcaggc gctgcagcgg gtggcgttgc agcgacgccg ccaacttcgt gccgagccgc
     1441 gggccgtcct gatcgggcct tcgagacagg atgtccgcga tcagcccgcc ctcgccggcc
     1501 tcga
//
LOCUS       DASWNJ010000653         3377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_682236, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000653 DASWNJ010000000
VERSION     DASWNJ010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682236"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="VGB85_10940"
     CDS             complement(<1..856)
                     /locus_tag="VGB85_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134592.1"
                     /translation="MIGTPRPGTIDTATIFRTIDTGTMPGTLRTPGALRERMLRTLGV
                     LVLLVVAVPGDSSAAPPPAVRGDSFSPTSGPAPAPAVSGDSSSPTPAAPRFTALVLRL
                     EGPVLADLGPALALRLPDLPIVAEAPPGAAPFVFVAVRSDPRAPSRHQLGVITSDGAA
                     YFREVDTGADAPARVLASVLANLLFSIAEGTVQPDRTDVEIPPQDAPLPDPEAPPAEP
                     TTTPTPPPAPAVEQPVPKDSPAPAPPRWDLGLVTSGVLGVPVGPPGFGPALAGGGGSL
                     GLDARGPRG"
     gene            complement(934..2016)
                     /locus_tag="VGB85_10945"
     CDS             complement(934..2016)
                     /locus_tag="VGB85_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134593.1"
                     /translation="MNDFNDLGELGDFLKDTRDHLDRALAESGAMPDLAASVARAREL
                     DPSAVSERAVDETAALAPVAQLRPKARRNTPVPENMSDRAVIDPFTAALRDQLEADLA
                     RQIHRGAIASASGPAVAPPAPIDLGRRRRTIVATVLALAAAVLITLGLGGAFEAQPAR
                     PGALPGAAALAGQRNAEAAAEAARPPRAVVVQAPPVPEDSPAAPVPEDSPEIGPAPDA
                     QDDLAPARPRPRRPVTPEPAAAPAEDPLAALDREAQLLWQQGKLREAADKFAEIVKRA
                     PGSRAAEAAYGDLFALEHRLGGDAVTLWRAYLRQFPRGRYADDARAGVCRRQPAAERG
                     ECWAEYLAAHPDGAHRREAEQATPAP"
     gene            complement(2013..2630)
                     /locus_tag="VGB85_10950"
     CDS             complement(2013..2630)
                     /locus_tag="VGB85_10950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY0134594.1"
                     /translation="MVTFAPEHPPADAGADALLAALVERARTGDMHAWARLYQDHWAH
                     LLRHVGYLTGDVTVAEDLVQETFAQALASLARYDARAPFFAWLRGIALNVVRKHWRTR
                     GRRTRAYTKLEALGDLASASDPEGGHLSKLRAQTLLAVLDDLPEHLREAFVLCDLRDM
                     STDAAAAELGISPGNLRVRATRARTRIREALTELGWLSPAEGGGK"
     gene            2810..>3377
                     /locus_tag="VGB85_10955"
     CDS             2810..>3377
                     /locus_tag="VGB85_10955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0134595.1"
                     /translation="MDSAQHSESPLNERDVFSYRRLFEAGETLICELDMEGRYLRLNT
                     PMSRVLGAEVAALSQRNYLEFVHPEDRAATEAAGREMASRPGDVVSFRNRYVRHDGQV
                     IWLAWQALADAESGRIYAVAQDVTARVQAEQAVVREHAAAAQAIAESESRLAEMSDHT
                     PALVYIKDPEGRYLFVNRYYEAVLGRRRA"
BASE COUNT          428 a         1106 c         1427 g          416 t
ORIGIN      
        1 ggccgcgagg accgcgagcg tcgaggccga gcgatccacc gccgcccgcg agcgcggggc
       61 cgaagcccgg cgggccgacg gggacgccga gcacgccgga ggtgacgagg ccgaggtccc
      121 agcgcggggg cgcgggtgcg gggctgtcct tcgggactgg ctgctcgacg gcgggcgcgg
      181 gaggtggggt gggcgtggtg gtcggctcgg ccggcggcgc ctcggggtcg ggcaaggggg
      241 cgtcctgcgg ggggatctcg acgtcggtgc ggtcgggctg gacggtgccc tcggcgatcg
      301 agaacaggag gttggcgagc acgctggcga gcacgcgcgc gggcgcgtcg gcgccggtgt
      361 cgacctcgcg gaagtaggcg gcgccgtccg aggtgatgac gccgagctga tgccgcgacg
      421 gcgcgcgcgg gtcggagcgc acggccacga acacgaaggg cgcggcgccg ggcggggcct
      481 cggcgacgat cgggaggtcg ggcaggcgca gcgcgagcgc ggggccgagg tcggcgagca
      541 ccggaccctc gaggcgcagc accagcgcgg tgaaacgcgg ggcggccggg gtgggggacg
      601 agctgtcccc ggagacagcg ggcgcggggg cgggcccgga cgtgggagag aagctgtccc
      661 cgcggacagc tggcggcggg gcggcggagc tgtcccccgg gacagccacg acgagcagca
      721 cgagcacgcc gagcgtccgc agcatgcgct cacggagcgc gccgggggta cgaagcgtgc
      781 cgggcatggt ccccgtgtcg atcgtccgga agatggtcgc cgtgtcgatc gttccgggcc
      841 tgggtgtgcc gatcacggcg gtggggtcgg ggggagccac gggcctggtg cgggcagacc
      901 ttgtcatgga tctccggggc tcgcacgcgg gcttcacggg gccggggtcg cctgttcggc
      961 ctcgcggcgg tgggcgccgt ccgggtgcgc ggcgaggtac tccgcccagc actcgccgcg
     1021 ctcggccgcg ggctggcggc ggcacacgcc ggcgcgggcg tcgtcggcgt agcggccgcg
     1081 tgggaactgg cgcaggtagg cgcgccacag ggtgaccgcg tcgccgccga ggcggtgctc
     1141 gagcgcgaac aggtcgccgt acgcggcctc ggcggcgcgg ctgcccggcg cgcgcttcac
     1201 gatctctgcg aacttgtcgg cggcctcgcg cagcttgccc tgctgccaca ggagctgggc
     1261 ctcgcggtcg agcgcggcca gcgggtcctc ggcgggggcg gccgcgggct cgggcgtgac
     1321 cggccgcctc gggcgcgggc gcgccggggc caggtcgtcc tgcgcgtccg gtgcgggccc
     1381 gatctccggg ctgtcctcgg ggacaggcgc ggcggggctg tcctcgggga caggtggcgc
     1441 ctggacgacg accgctcgcg gggggcgagc ggcctcggcg gcggcctcgg cgttgcgctg
     1501 gccggcgagg gcggccgcgc ccggcagcgc gcccgggcgg gcgggctggg cctcgaaggc
     1561 gccgccgagg ccgagggtga tcagcacggc ggcggccagg gcgaggaccg tggcgacgat
     1621 cgtgcgccgg cgccggccga ggtcgatcgg cgccggcgga gcgacggcgg gtcccgaggc
     1681 gctggcgatc gcgccgcggt ggatctggcg cgcgaggtcg gcctcgagct ggtcgcgcag
     1741 cgccgcggtg aatggatcga ttactgcccg atccgacatg ttctcgggga caggtgtgtt
     1801 gcgccgcgcc ttcggtcgca gctgggcgac cggggcgagc gcggccgtct cgtcgacggc
     1861 tcgctccgag accgcggacg ggtcgagctc gcgggcgcgg gcgaccgagg cggcgaggtc
     1921 gggcatcgca ccgctctcgg cgagggcgcg gtcgaggtgg tcgcgggtgt ccttgagaaa
     1981 gtcgccgagc tcaccgaggt cgttgaagtc gttcacttgc cacctccctc ggccgggctc
     2041 agccagccca gctcggtcaa cgcctcacgg atgcgagtcc gggcccgtgt ggctcgcacg
     2101 cgcaggtttc cgggggagat ccccagctcg gcggcggccg cgtcggtcga catgtctcgc
     2161 agatcacaga ggacaaacgc ctctcgcagg tgctcgggga gatcgtcgag gaccgcgagc
     2221 aatgtctgcg cgcgcagctt ggacaggtgg ccgccctcgg ggtcgctcgc gctagcgagg
     2281 tcgccgagcg cctcgagctt cgtgtacgcg cgggtacgac ggccgcgggt gcgccagtgt
     2341 ttacggacta cattgagagc gatgccgcgg agccacgcga agaagggggc gcgggcgtcg
     2401 taacgggcca gactggcgag cgcctgggca aaggtctcct gcacgaggtc ctcggcgacg
     2461 gtcacgtcgc cggtcaggta accgacgtgg cgcaggaggt gggcccagtg gtcctggtag
     2521 agccgcgccc atgcgtgcat gtcgccggtc ctggcgcgct cgacgagcgc ggcgaggagc
     2581 gcgtcggcgc ccgcatcggc ggggggatgt tcaggagcga aggtcaccat ggagactgcc
     2641 cgagttcccg caggtcgggc aatgttaccg ggtgccggac gaattcaggc caggggagcc
     2701 gggggggccg cagatgacgg cgtgggtcgg gccgatgcgc gacaatggcc ggcaatttat
     2761 tgggcctatt gggcgccggg tcggtcaaga tagcgacgac cgacagggaa tggacagcgc
     2821 ccagcacagc gagtcgccgc tcaacgagcg cgacgtgttc agttaccgcc gcctgttcga
     2881 ggccggcgag acgctgatct gcgagctcga catggagggc cggtacctgc ggctcaacac
     2941 gccgatgtcc cgcgtgctcg gggccgaggt cgcggcgctg agccagcgca actacctcga
     3001 gttcgtgcac ccggaggacc gggcggccac cgaggcggcg ggccgcgaga tggcgagccg
     3061 ccccggcgac gtcgtcagct tccgcaaccg ctacgtgcgt cacgacggtc aggtgatctg
     3121 gctggcctgg caggcgctcg ccgacgccga gtcgggccgg atctacgcgg tcgcgcagga
     3181 cgtcacggcg cgcgtgcagg ccgagcaggc ggtggtgcgt gagcacgccg cggccgccca
     3241 ggcgatcgcc gagagcgagt cgcggctggc cgagatgtcc gaccacacgc cggcgctcgt
     3301 ctacatcaag gaccccgagg gccgctacct gttcgtcaat cgctactacg aggcggtgct
     3361 cggccggcgg cgcgccg
//
LOCUS       DASWNJ010000654         2043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_542391, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000654 DASWNJ010000000
VERSION     DASWNJ010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542391"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VGB85_10960"
     CDS             <1..927
                     /locus_tag="VGB85_10960"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEY0134596.1"
                     /translation="TCDGSLGTMEAPFCKIGDAFAKIAGDDVTLGWTVKIKGGNYLEE
                     PLFVPDGATVVLTRWGDTSPKIRALDDSGATLTIQNGSKGYIDRLAFNSNDSNHGVVC
                     ANATVWIDDTRIAGNKGEGYDSTDCTSRIDRSVIFDNDGGGIASYGAGTTHIANSYIS
                     GNGTQNGGDFGGIRSAQGNELHLIYSTVVNNLSAAGPRTLQCVDAGTVEVRNSVLIGF
                     GLPSVDCPTATFTTSAVDEGTVEGDGNLIAVQMDIMNFFNAPNAGVYTALADTAISML
                     AVWKEGDPKTDFNGTPRANMDGAMDYAGADRP"
     gene            complement(1022..>2043)
                     /locus_tag="VGB85_10965"
     CDS             complement(1022..>2043)
                     /locus_tag="VGB85_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134597.1"
                     /translation="AHALHHFEAYLRAPDLSADDRALAESRARAAAAGTTLLTVVVRS
                     ADAPVPGTRVTASFTDAPDGQDRPPLAVEAPPVTSAEGLSYALRLDPGRWQITVAAPG
                     RPAAKAIAEVTAGVPARAYVTLAPPGTTVATPATPVEATNATSSPSSPSSPSSSSRSS
                     TPVVAGLAAGAVVGVGAGVALTTIGSRRFAAAFVPCPGEPGCSESEIFADVRMTGAGT
                     ALLGAGLGLAVSATAARFMRTPTPWYAQLGLGGALLLTGTAWTIASNLRHRDIDDITR
                     HAGSIDAWLAGRSVGTAVLGAGAGLVVGSGVALLGLRRARARHLALAPVAGGGQLGLR
                     LLGRF"
BASE COUNT          315 a          745 c          743 g          240 t
ORIGIN      
        1 acctgcgacg gcagcctcgg cacgatggag gccccgttct gcaagatcgg cgacgccttc
       61 gccaagatcg ccggggacga tgtcacgctc ggctggaccg tcaagatcaa gggcggcaac
      121 tacctcgagg agcccctctt cgtgccggac ggcgcgaccg tggtgctgac gcgctggggc
      181 gacacctcgc cgaagatccg cgcgctcgac gacagcggcg cgaccctgac gatccagaac
      241 ggctccaagg ggtacatcga ccgcctcgcg ttcaacagca acgacagcaa ccatggtgtc
      301 gtctgcgcca acgcgaccgt gtggatcgac gacacccgca tcgccggcaa caagggcgag
      361 ggctacgact cgaccgactg cacctcgcgg atcgaccgct cggtgatctt cgacaacgac
      421 ggcggcggca tcgcctcgta cggcgcgggc acgacccaca tcgccaacag ctacatcagc
      481 ggcaatggca cccagaacgg cggcgacttc ggcggcatcc gcagcgccca gggcaacgag
      541 ctgcacctga tctactcgac cgtcgtcaac aacctcagcg ccgcgggtcc gcggaccttg
      601 cagtgcgtcg acgccggcac cgtcgaggtc cgcaactcgg tgctgatcgg cttcggcctg
      661 ccgtcggtcg actgcccgac cgccacgttc acgacctccg cggtcgacga gggcaccgtc
      721 gagggcgacg gcaacctcat cgccgtgcag atggacatca tgaacttctt caacgcgccg
      781 aacgccggcg tctacacggc cctcgccgac accgcgatct cgatgctcgc cgtgtggaag
      841 gaaggcgacc ccaagaccga cttcaacggc acgccgcgcg ccaacatgga cggcgcgatg
      901 gactacgccg gcgccgacag gccctgacat ctcgcggccc cgtgccgacc cgggcgcctc
      961 gccgttcgtc caccggacgc acgggaggcg ccttcgctgc gtccgctgac ctcgcggtcg
     1021 cctagaagcg gccgagcagg cgaaggccga gctgtccgcc gccggcgacc ggcgcgagcg
     1081 cgaggtggcg ggcgcgggcc cgacggagcc cgagcagcgc gaccccggag ccgaccacga
     1141 ggccagcgcc ggcgccgagc accgcggtcc cgacgctgcg tccggccagc cacgcgtcga
     1201 tgctgcccgc gtggcgggtg atgtcgtcga tgtcgcggtg gcgcaggttg ctggcgatcg
     1261 tccacgccgt gccggtcagc agcagcgcgc cgccgaggcc gagctgggcg taccacggcg
     1321 tcggcgtgcg catgaagcgg gccgcggtcg cagagacggc gagcccgagc ccggccccga
     1381 gcaacgcggt gcccgctccg gtcatgcgca cgtcggcgaa gatctcggac tcgctgcagc
     1441 cgggctcgcc agggcagggc acgaacgcgg cggcgaagcg acgcgagccg atcgtcgtca
     1501 gcgcgacgcc ggcaccgacg ccgacgaccg ccccggcagc gaggccggcg accacgggcg
     1561 tcgacgatct cgatgaggat gatggcgatg atggcgatga tggcgatgac gtcgcgttcg
     1621 tcgcctcgac cggcgtcgcg ggtgtggcga cggtggtccc cgggggcgcg agcgtgacgt
     1681 atgcgcgggc gggcacaccg gcggtcacct cggcgatcgc cttcgcggcc ggccgcccgg
     1741 gggcggcgac ggtgatctgc cagcggcccg gatcgaggcg cagcgcgtag gacaggccct
     1801 ccgcgctggt gaccggcggc gcctcgacgg ccagcggggg acggtcctgc ccgtccggcg
     1861 cgtcggtgaa gctggccgtg acgcgggtgc cggggacggg cgcgtccgcg gagcgcacga
     1921 cgacggtcag cagcgtcgtc cccgccgcgg cggcccgggc ccgactctcg gccagcgcgc
     1981 ggtcgtcggc gctcaggtcc ggggcgcgca ggtaggcctc gaagtgatgc agcgcgtggg
     2041 ccc
//
LOCUS       DASWNJ010000655         1601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_81860, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000655 DASWNJ010000000
VERSION     DASWNJ010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81860"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VGB85_10970"
     CDS             <1..602
                     /locus_tag="VGB85_10970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004412.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEY0134598.1"
                     /translation="HRPGRRLHLHLMSVASLLLAVEEGGEGHNPLIPNANELFWGTIC
                     FAIFVGFMVKFVLPKAREALEQRTAGIEGKLEQAERDRDEAQQLLAQYRQQLAEARSE
                     AGRIRTEAQAQRAQIVEEARVEARTEAARITEAATAQIASERQQVLAELRRDVGGLAV
                     TLASKVVGESLEDEARQRRTVERFLDGLEATPTTPAAVR"
     gene            602..1408
                     /locus_tag="VGB85_10975"
     CDS             602..1408
                     /locus_tag="VGB85_10975"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEY0134599.1"
                     /translation="MQGASRAALAHSIAAFEASPATSSAEVSEGLYAVAALLDREPSL
                     RRAFTDPASSPDARRGLAQALLGRQLSEAPLGVLSELVSQGWSSPADLRAAVEVLAAD
                     AALRAAEHDGVLDDVEDELFRFARLLEREPALRAALTDPGLPDDRKSGLLRELLGERA
                     RPQTLRLVEIAVTRSRGRSVETALDELADLAAQRRQRFVAQVRVARPLDLQQEARLSA
                     SLERLYGRRVQLQVDVDPAVLGGISVRVGDEVLDGTVQRNLEAVRRNLAG"
BASE COUNT          211 a          605 c          587 g          198 t
ORIGIN      
        1 ctcatcggcc tggtcgccgg cttcatcttc acctgatgtc cgtcgccagc ctgctgctcg
       61 ccgtcgagga gggcggtgag ggccacaacc cgctcatccc gaacgccaac gagctgttct
      121 ggggcacgat ctgcttcgcg atcttcgtgg ggttcatggt caagttcgtg ctgccgaagg
      181 cccgtgaggc gctggagcag cggacggcgg gcatcgaggg caagctcgag caggccgagc
      241 gcgaccgcga cgaggcccag cagctgctgg cgcagtaccg ccagcagctg gccgaggccc
      301 gcagcgaggc cggccggatc cgcaccgagg cgcaggccca gcgcgcgcag atcgtcgagg
      361 aggcgcgggt cgaggcccgc accgaggcgg cgcgcatcac tgaggcggcg accgcccaga
      421 tcgccagcga gcgccagcag gtgctcgccg agctgcgtcg cgacgtcggc ggtctggccg
      481 tcacgctcgc gagcaaggtc gtcggcgagt cgctcgagga cgaggctcgc cagcgccgca
      541 cggtcgagcg cttcctcgac gggctcgagg ccaccccgac cacgcccgcg gcggtccgct
      601 gatgcagggc gccagccgcg cggcgctcgc gcactcgatc gcggcgttcg aggcgtcccc
      661 cgcgacgagc tcggcggagg tcagcgaggg cctgtacgcc gtggcggccc tgctcgaccg
      721 ggagccgtcg ctgcgccgtg ccttcaccga cccggcgagc tcgccggacg cgcggcgcgg
      781 gctggcgcag gccctgctgg gtcgtcagct cagcgaggcg ccgctggggg tgctgtccga
      841 gctcgtctcg cagggctgga gctcgccggc cgacctgcgc gctgcggtgg aggtcctcgc
      901 ggccgacgcg gccctgcggg cggctgagca cgacggggtc ctcgacgacg tcgaggacga
      961 gctgttccgc ttcgcgcgtc tgctcgagcg cgagccggcg ctgcgcgccg ccctcaccga
     1021 cccggggctg cccgacgacc gcaagtccgg gctgctgcgc gagctgctcg gcgagcgggc
     1081 ccggccgcag accctgcgcc tggtcgagat cgccgtcacg cgctcgcgcg gccgctccgt
     1141 cgagaccgcc ctcgacgagc tggccgacct cgccgcccag cgccgtcagc gcttcgtcgc
     1201 ccaggtgcgg gtcgcgcggc ccctggacct gcagcaggag gcgcggctgt ccgcctcgct
     1261 cgagcgcctg tacggccggc gcgtccagct ccaggtcgac gtcgaccccg ccgtcctcgg
     1321 cggcatctcg gtccgcgtcg gggacgaggt cctcgacggc accgtgcagc gcaacctcga
     1381 ggccgtccgg cgcaacctcg ccggctgacc tcgaccgctc gacctgaagt ccccgacagc
     1441 cccacgcgac accacagacc cagagagcag ggatccacag atgacggagc tcagcatccg
     1501 cccggaggac gtccggtccg cgatcgagca gtacgtcacg tcgtacagcg ccgagaccac
     1561 ccgcgaggag gtcggcaccg tcgccgaggt cggtgacggc a
//
LOCUS       DASWNJ010000656         2482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_364263, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000656 DASWNJ010000000
VERSION     DASWNJ010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364263"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2235)
                     /locus_tag="VGB85_10980"
     CDS             complement(<1..2235)
                     /locus_tag="VGB85_10980"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HEY0134600.1"
                     /translation="MPRHVLCHRPSARLSARLSARLCARLVPVLLTALVVAATGGKVR
                     ADEGQWMPSQIAELDQQRLREMGLELTPEQLWGAEAGQHGGLMRAAVNLSGCSAAFIS
                     ADGLVATNHHCAYGAIQANSSVEHDYLKDGFLARSRDQELHGKDLNVRVLERIDDVTA
                     EVKAAADAATDDRARAQAIERTRKQLIEACEKSGPSLRCDVTSFYSGSLYQRFTYREL
                     RDVRLVYAPPAAVGEFGGEVDNWMWPRHTGDFTLLRVYAGADNAPAEHAASNVPYKPQ
                     QWLKPAHEGVAAGDFVAVLGYPGLTQRYLPVVEAERHVEQVFPARIDLYGEWISIYEE
                     LGGRDPAVGIKVAASKKQLANRHKNAQGMIAGLRAMKLVERRRDEEVQLEAWAAQPGN
                     PGFGDVLPEIRAISQDRREAFPAEFLLENLSNAGNLLPIAIDLVRRAREAKKPDLERL
                     AGYMDRDAKRLRDRLERRLRDYDAEVDVQVLAALLARVAVLPDNAGIPALAALTAGAT
                     GKRDAFVPAARKLIAASKLADEATLALLAADEETLARSTDPLLVLARELVPALEALDA
                     RNEARTGRGFKVGPRYFTMLRTIRKGPVYPDANGTLRFSYARIEGYSPREGLIATPQT
                     TLAGQMAKVTGVAPFDMPARIVDIAGAAAQTYWSDPVLGDLPINFLANGDTTGGNSGS
                     PVINGRGELVGLNFDRVWENIAGDFGYNPARSRNVIVDLRYLLWLLDRAEDAGDLLAE
                     LGMSQ"
     gene            complement(2269..>2482)
                     /locus_tag="VGB85_10985"
     CDS             complement(2269..>2482)
                     /locus_tag="VGB85_10985"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HEY0134601.1"
                     /translation="WQHNWPLFIGGQVVGWAFQLAGHRIEGNRPALLKRPISFLMGPL
                     MVLVEAAESLGLHFGFARRAREALGL"
BASE COUNT          354 a          872 c          884 g          372 t
ORIGIN      
        1 ctgcgacatg ccgagctcgg cgagcaggtc accggcgtcc tcggcccggt cgagcagcca
       61 caacaggtag cgcaggtcga cgatgacgtt gcgcgagcgc gcggggttgt agccgaagtc
      121 gccggcgatg ttctcccaca cgcgatcgaa gttgaggccg acgagctcgc cgcgcccgtt
      181 gatcaccggg ctgcccgagt tgccgccggt cgtgtcaccg ttggcgagga agttgatcgg
      241 caggtcgccg agcaccgggt cggaccagta ggtctgcgcg gccgcaccgg cgatgtcgac
      301 gatgcgcgcg ggcatgtcga agggcgcgac gccggtgacc ttggccatct gaccggcgag
      361 ggtcgtctgc ggggtggcga tcaacccctc gcgcgggctg tagccttcga tcctggcgta
      421 cgagaagcgc agggtgccgt tggcgtccgg atacaccggt cccttgcgga tcgttcgcag
      481 catcgtgaaa tagcgtggac cgaccttgaa gccgcggccc gtgcgggcct cgttgcgcgc
      541 gtccagggcc tcgagcgcgg gcaccagctc gcgcgcgagc accagcagcg ggtccgtgct
      601 gcgcgccagt gtctcctcgt cggcggcgag cagcgcgagc gtcgcctcgt cggcgagctt
      661 gctcgcggcg atcagcttgc gcgccgcggg cacgaaggcg tcgcgcttgc cagtcgcgcc
      721 cgcggtcagg gccgcgagcg ccgggatgcc ggcgttgtcc ggcaacaccg cgacgcgggc
      781 cagcagcgcg gcgagcacct gcacgtcgac ctcggcgtcg taatctcgca ggcgccgctc
      841 gaggcggtcg cgcaggcgct tggcgtcgcg gtccatgtag ccggcgaggc gctcgaggtc
      901 cggctttttg gcctcgcgcg cgcgccgaac caggtcgatc gcgatcggca gcaggttgcc
      961 ggcgttggaa agattctcga gcaggaactc ggccgggaac gcctcgcggc ggtcctggga
     1021 gatcgcgcgg atctccggga ggacgtcgcc aaagccgggg ttgccgggct gcgcggccca
     1081 ggcctcgagc tgcacctcct cgtcgcgccg gcgctcgacc agcttcatcg cccgcagccc
     1141 cgcgatcatc ccctgggcgt tcttgtggcg gttggcgagc tgcttcttgc tggccgcgac
     1201 cttgatcccg accgcggggt cgcgcccgcc gagctcctcg tagatcgaga tccactcgcc
     1261 atacagatcg atgcgcgccg ggaacacctg ctcgacgtgc cgctcggcct cgacgaccgg
     1321 caagtagcgc tgcgtcagcc ccggatagcc gagcaccgcc acgaagtcgc ccgccgcgac
     1381 gccctcgtgc gccggcttca accactgttg aggtttatac ggcacgttgc tcgcggcgtg
     1441 ctcggccggc gcgttgtcgg ccccggcgta cacgcgcagc agcgtgaagt cgccggtgtg
     1501 ccgcggccac atccagttgt cgacctcgcc gccgaactcg ccgaccgcag ccggcggcgc
     1561 gtagaccagc cgaacgtcgc gcagctcgcg atacgtgaag cgctggtaca ggctgccgct
     1621 ataaaaactg gtgacgtcgc agcgcaggct cgggcccgac ttctcgcacg cttcgatcag
     1681 ctgcttgcgc gtgcgctcga tcgcctgggc ccgggcgcgg tcgtcggtcg ccgcgtcggc
     1741 ggccgccttg acctcggcgg tgacgtcgtc gatgcgctcg agcacccgca cgttgaggtc
     1801 cttgccgtgc agctcctggt cccggctgcg cgcgaggaag ccgtccttga ggtagtcgtg
     1861 ctcgacgctg ctgttggcct ggatcgcacc gtaggcgcag tgatggttgg tggcgacgag
     1921 gccgtcggcg gagatgaagg cggccgagca gccgctcagg ttgaccgcgg cgcgcatcag
     1981 cccgccatgt tggccggcct cggcgcccca cagctgctcg ggcgtcagct cgagccccat
     2041 ctcgcgcaac cgctgctgat cgagctcggc gatctgcgag ggcatccact ggccctcgtc
     2101 cgcacggacc ttgccacctg tcgccgccac cacgagggcg gtcagcagga ccgggacgag
     2161 gcgcgcacag agtcgcgcag agagccgcgc agagagccgc gcagaggggc ggtggcagag
     2221 gacgtgccgg ggcatgttcg ccaccttaca ccagaatccc gccccttctt acagccccag
     2281 cgcctcgcgg gcccggcgcg caaagccgaa gtgcaggccg agggactcgg cggcctcgac
     2341 cagaaccatc agggggccca tcaaaaagga gatcggccgc ttgagcagcg ccgggcggtt
     2401 gccctcgatg cgatgtccgg caagctggaa cgcccagccg accacctgcc cgccgatgaa
     2461 cagcggccag ttgtgctgcc ac
//
LOCUS       DASWNJ010000657         2738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_260251, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000657 DASWNJ010000000
VERSION     DASWNJ010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260251"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VGB85_10990"
     CDS             <1..696
                     /locus_tag="VGB85_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134602.1"
                     /translation="TLTTGGDPTGAACLLNDEAAVLVRMGHPGRALQLLRESRKVFDA
                     HASDDPVALRELAETELLFARLPLHAQMRPGREHEGFTMGLDHALAAERAYHELGEAR
                     ELGRVWETMGRLELRRGRIEAARQRLEAAAEVQAQLGDLTGLASTTEALSEVLSQCGR
                     DAEAVTLLRDSIVFNRDKGSPLGLMGNRRAYTALAGRLAGQRQHAAGLQEAAILLTAG
                     ERELGVQPAQAAR"
     gene            764..1960
                     /locus_tag="VGB85_10995"
     CDS             764..1960
                     /locus_tag="VGB85_10995"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEY0134603.1"
                     /translation="MTDPDIARDLAAIARIEAVPTILRTIRESTGLRFTLIARVLPDR
                     WVACAVHDELDFGLRPGSELEVATTLCSQVRDTHEPVVIDHVSHDTGYCDHPTPKMFG
                     FESYIAVPIFRQSGEYFGNICGLDPEPRTLSDAKTLNMLRLFSQLISLQLAAEDRHQR
                     SQAELGQQRETSELREQFIAVLGHDLRNPLSSIGMGTELLLRRSTDAGDRRVLERIRS
                     SARRIGALVDDVMDLARGRFGGGIKLRTGAVTDLATRLRHVIDEVQAAHPNRPIELST
                     TLAGPVTCDEQRVEQLLSNLLTNAIAHGDADTPITVALRGEADAIELAVTNLGAAISE
                     EARPRLFQPYFRGGRSNPRDGLGLGLYIVSEIARAHGGRVDVDSADSRTTFTLRMPRV
                     AAAASA"
     gene            2019..>2738
                     /locus_tag="VGB85_11000"
     CDS             2019..>2738
                     /locus_tag="VGB85_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134604.1"
                     /translation="MSSSQHRWALAVTLLVHCGGAGTCPDCGEPARRGVLARAELDEV
                     SGIVASSRLPDVLYVHNDAGDSSRFFAISPAGADLGVFAVKGAQNVDWEDMGRGPCPA
                     GSCLYIGDIGDNDRDRLAYALYRIVEPTTISPGEHSVDADRLVFTYPDGAHDAETLLV
                     HPVTGEVTIVTKVDSGTAQIFVLPRDLTTTKTLMATAAGELVSPSGDDRFTGGAIHPD
                     GVAVLLRTSGGLFWYPMQPAQT"
BASE COUNT          393 a         1061 c          916 g          368 t
ORIGIN      
        1 accctcacca ccggcggcga cccgaccggc gccgcctgcc tgctcaacga cgaggccgcc
       61 gtgctcgtcc gcatgggcca cccggggcgc gccctgcagc tgctccgcga gtcgcgcaag
      121 gtgttcgacg cccacgccag cgacgacccg gtcgcgctgc gcgagctcgc cgagaccgag
      181 ctgctgttcg cccgcctgcc gctgcacgcc cagatgcgcc ccggccgcga gcacgagggc
      241 ttcacgatgg gcctcgacca cgccctcgcg gccgagcgcg cctaccacga gctcggcgag
      301 gcccgcgagc tcgggcgcgt atgggagacg atgggccggc tcgagctgcg ccgcggtcgc
      361 atcgaggcgg cgcgccagcg gctcgaggcc gcggccgagg tgcaggcgca gctcggcgac
      421 ctgaccggcc tggcgagcac cacggaggcc ctgtcggagg tgctctcgca gtgcggacgc
      481 gacgccgagg ccgtgaccct gctgcgcgac tcgatcgtgt tcaaccgcga caagggctcg
      541 ccgctgggcc tgatgggcaa ccgccgggca tataccgcgc tcgccgggcg cctcgccggc
      601 cagcgccagc acgccgccgg ccttcaggag gccgcgatcc tgctgaccgc cggggagcgc
      661 gagctcggcg tacagcccgc gcaggcagcc cgctgaccgc ccggaccgga ccgcgtgaaa
      721 gccccgctgc gccgggccga cagccgtggt atccggatct cggatgaccg atccggacat
      781 cgcccgggac ctcgccgcga tcgcgcggat cgaagccgta ccgacgatcc tgcgcacgat
      841 ccgtgagtcg accggcctgc gcttcacctt gatcgcgcgg gtgctgcccg accgctgggt
      901 cgcctgcgcc gtgcacgacg agctcgactt cgggctgcgg ccaggcagcg agctcgaggt
      961 cgcgacgacc ctgtgcagcc aggtccgcga cacccacgag ccggtggtga tcgaccacgt
     1021 cagccatgac accggctatt gcgaccaccc gacgccgaag atgtttggct tcgagagcta
     1081 catcgccgtg ccgatcttcc gccagtccgg cgagtacttc ggcaacatct gcggcctcga
     1141 tcccgagccg cgcacgctga gcgacgccaa gaccctcaac atgttgcgcc tgttcagcca
     1201 gctgatctcg ctgcagctcg ccgccgagga ccgccaccag cgcagccagg ccgagctcgg
     1261 ccagcaacgc gagacctccg agctgcgcga gcaattcatc gccgtgctcg gccacgacct
     1321 gcgaaacccg ctgtcgtcga tcggcatggg caccgagctg ctgctgcgca ggagcaccga
     1381 cgccggcgac cgccgggtgc tcgagcgcat tcgttcgagc gcccgccgga tcggcgcgct
     1441 cgtcgacgat gtcatggacc tcgcgcgcgg ccgcttcggc ggcggcatca agctccggac
     1501 cggcgcggtc acggacctgg cgacgcggct gcggcacgtg atcgacgagg tccaggccgc
     1561 gcacccgaac cgcccgatcg agctgtcgac gacgctcgcg gggcccgtca cctgcgacga
     1621 gcagcgcgtc gaacaattgc tgtccaacct gctcaccaac gcgatcgccc acggcgacgc
     1681 cgacaccccg atcaccgtcg cgctgcgcgg ggaagcggac gccatcgagc tggcggtcac
     1741 caacctcggc gcggcgatct ccgaggaggc ccgcccgcgc ctgttccagc cctactttcg
     1801 cggcggacgc agcaaccccc gcgacggcct cggcctcggc ctctacatcg tctcggagat
     1861 cgcacgcgcc cacggcggtc gcgtcgacgt cgactcggcc gacagccgca ccaccttcac
     1921 attgcgcatg cctcgcgtcg ccgccgccgc ttccgcctga ggcccgcgat cgcgccgcct
     1981 cccacggcgg gtgcccctcg tgataatcat ggcgtggcat gtcctcctcg cagcaccgct
     2041 gggccctcgc cgtgacgctg ctcgtgcact gtggcggcgc cgggacctgc ccggactgcg
     2101 gcgagccggc gcgccggggc gtgctggccc gggccgagct ggacgaggtc tccggcatcg
     2161 tcgccagctc gcggctgccg gacgtgctct acgtgcacaa cgacgccggc gactcctcgc
     2221 gcttctttgc gatctccccg gcgggcgcgg acctcggtgt gttcgcggtg aagggcgcgc
     2281 aaaacgtcga ctgggaggac atgggtcgcg gcccgtgtcc ggcgggcagt tgcctgtaca
     2341 tcggcgacat cggcgacaac gaccgcgatc gcctggcata cgccctgtat cgcatcgtcg
     2401 agccgaccac gatctcgccc ggcgagcact cggtcgacgc cgaccggctg gtcttcacct
     2461 accccgacgg tgcccacgac gccgagacgc tgctcgtcca cccggtcacc ggcgaggtga
     2521 cgatcgtgac caaggtcgac tccggcacgg cgcagatctt cgtattgccg cgcgacctga
     2581 cgaccacgaa gacgctgatg gccaccgcgg ccggcgagct cgtatcaccc tccggcgatg
     2641 accgcttcac cggcggagcg atccaccccg atggtgtcgc ggtcctgctg cgcacctccg
     2701 gcggcctgtt ctggtacccg atgcagccgg cgcagacc
//
LOCUS       DASWNJ010000658         3792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_577414, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000658 DASWNJ010000000
VERSION     DASWNJ010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577414"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3208)
                     /locus_tag="VGB85_11005"
     CDS             complement(<1..3208)
                     /locus_tag="VGB85_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134605.1"
                     /translation="MATDDLIAALQGVGRVDSEGSFSFDREKAREKLRTFQVAEPQRY
                     VLHFIAVAALKGATKIEVLCDSDDLIVRFDGAAITSEDLDDLYNASFAAAHSDEQRAR
                     QQLAVGIHAALALNPKHVRIVSGADGTGVSLLASHDVADVVAPAKDARGTQIHVKQRF
                     RPGLMIRFVRHLRGGLAEATWLRARGCHATVPITLNGELVSAGLTLREAQHCQSGSDG
                     HRRGVAGLLRHHEPGVIRLVRHGVWICDETRAWLPPGIVVVIADDRLLTDLSGDRVVL
                     DAEHERCMVLARQLAAEVVDAAIGPGPAADPEPALRERLHGVWRTWDGALNPWTTIGA
                     AMTRILAWHDIWGRRHTFAALLAEVERVGLLMTTTGDFTGRVPPTDEIILRTGDEFTD
                     AVLALGLGGRMRDFTAQLEARAHAEANRRRWRAQPCEPTLTAANYLMIADFSVRVDER
                     WVRGQAGLRRVPAAHCRLRVIVDGCALAELDLLAPIAGVDVVLAGPLEILADYSGPTR
                     DALFAAALAGWTATARVLVEAAMRRGVLDWKPSPAAQALVRGFVRAHQPGDALRSVLV
                     AAGYEATAADQHAQVLAARIPAPAVPQGLAQQPWLRDSFHFTTASGLTLSVGAVAVAL
                     AHGMPVACVSDVVEPRPALKEVVLRVAPWEEELLGWLFGDGVTMLSPAEYELRCAEAD
                     FMGRPTRPLALDPETHTDSVVVEQDGLRVAMAFPRAADGWDANHALSPCEIVRAGRTL
                     TQLKIWSPVPGASFAIVGDSLTPRPDWEGVRLDDAFRAAIVKTTAAVPALVRAAIEGA
                     SEAAEDDNDDIYEAWRRGVLAALVADFPSAQMRSAYAHLLTALGRKQAEEQYLELLAL
                     GASRSMHVLSRAIEKYTAEVGVLIDVGELAARISAPHPTSEQVGHVADVLRDIRAELG
                     QAGGSSWIDRVVNFSPEVGALPLFRRVDRSAMTLAEAAAEAKTDLLHVYVATGGKPGR
                     EYQRSLRVDPIVFEQLRCLFGPTRVTTAPPREEPEQRPVQRAETRRSKPERPRPKQEI
                     EVDPPPTPTDLAALFAELEREADARPAPTLPVSSSKPDA"
     gene            complement(3190..>3792)
                     /locus_tag="VGB85_11010"
     CDS             complement(3190..>3792)
                     /locus_tag="VGB85_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134606.1"
                     /translation="KAAKARRVYWAAAASPLTRALHDRGDLVIAAEGDDSTAVTVEAS
                     AAWVCALPRLLAGRPAPPLHTALCTSLPVADPPHWSRLRPVLATLLSEVGPRPRAITA
                     GDLRYPGSAVAGRVAIAQDDLGAVTALDRLELVAGQRLIVHVAHPAVASAVALAEREP
                     ELAAYVLAKLVVLGHGRGLSIEADHTLAAKALELRWQRMT"
BASE COUNT          506 a         1452 c         1352 g          482 t
ORIGIN      
        1 cggcgtcggg cttcgacgac gacaccggca gcgtcggggc cggcctcgcg tcggcctcgc
       61 gctcgagctc ggcgaacagc gcggcgaggt cggtgggggt cggcggtggg tcgacctcga
      121 tctcctgctt cggccgcggc cgctcgggct tgctgcgcct cgtctcggct cgctggaccg
      181 gccgctgctc gggctcctcg cgcggcgggg cggtcgtgac ccgcgtcggg ccgaacaggc
      241 agcgcagctg ttcgaacacg atcggatcga cacgcagcga tcgctggtac tcgcggcccg
      301 gcttgccgcc ggtggcgaca tacacgtgca gcaggtcggt cttcgcctcg gcggccgcct
      361 ccgcgagcgt catcgccgag cggtcgacgc ggcgaaacag cggcagcgcg ccgacctcgg
      421 gcgagaagtt gaccacgcga tcgatccacg agctgccgcc ggcctgcccg agctccgccc
      481 ggatgtcgcg cagcacatcg gcgacgtgcc cgacctgctc gctcgtcgga tgcggcgcgg
      541 agatccgggc ggccagctcg cccacgtcga tcagcacgcc gacctccgcg gtgtacttct
      601 cgatcgcgcg gctgaggacg tgcatgctgc gcgacgcccc gagcgcgagc agctcgaggt
      661 actgctcctc cgcctgcttg cggccgagcg ccgtcagcag gtgcgcgtac gcggaccgca
      721 tctgcgcgga ggggaagtcg gcgaccagcg cggccagcac accgcgccgc caggcctcgt
      781 aaatgtcgtc attgtcgtcc tcggcggcct cgctggcccc ctcgatcgcc gcgcgcacca
      841 gcgccggcac ggccgcggtc gtcttcacga tcgccgcccg gaacgcgtcg tcgagccgca
      901 cgccctccca gtccgggcgc ggcgtcagcg agtcgccgac gatcgcaaag ctggccccgg
      961 ggacaggtga ccagatcttg agctgcgtca gggtccggcc ggcgcgcacg atctcgcagg
     1021 gcgacagggc atggttggcg tcccagccgt cggccgcgcg cgggaaggcc atcgccacgc
     1081 gcagcccgtc ctgctcgacg accacgctgt cggtgtgggt ctccgggtcg agcgcgagcg
     1141 gccgcgtcgg tcgacccatg aagtcggcct cggcgcagcg cagctcgtac tccgcgggcg
     1201 acagcatcgt gacaccgtcg ccgaacagcc acccgagcag ctcctcttcc cacggagcga
     1261 cgcgcagcac cacctccttg agcgccggac gcggctcgac cacgtcggac acgcaggcga
     1321 ccggcatccc gtgcgccagc gccaccgcga ccgcgccgac gctgagcgtc aggccggacg
     1381 cggtcgtgaa gtgaaagctg tcgcggagcc acggctgttg cgcgaggccc tgcgggaccg
     1441 ctggcgccgg aatgcgagct gccagcacct gcgcgtgctg gtcggccgcc gtcgcctcgt
     1501 aacccgcggc cacgagcacg ctgcgcagcg cgtcgcccgg ctgatgcgcg cggacgaagc
     1561 cacggaccag cgcctgcgct gccggcgagg gcttccagtc gagcacgccg cggcgcatcg
     1621 cggcctcgac gagcacgcgc gccgtcgcgg tccagcccgc gagcgccgcg gcgaacaggg
     1681 cgtcgcgcgt cggcccggag taatccgcga ggatctccaa tggacccgcg agcacgacgt
     1741 cgacgccggc gatcggcgcg agcaggtcga gctccgcgag cgcgcaacca tcgacgatca
     1801 cgcgcaggcg gcaatgcgcg gccggcaccc ggcgcaggcc cgcctgcccg cggacccatc
     1861 gctcgtcgac ccgcacgctg aagtcggcga tcatcagata gttcgcggcg gtcagcgtcg
     1921 gctcgcaggg ctgcgcccgc cagcgccgac ggttggcctc cgcgtgcgcg cgtgcctcga
     1981 gctgcgcggt gaagtcgcgc atgcgaccgc cgagcccgag cgccagcacg gcatccgtga
     2041 attcgtcgcc ggtccgcagg atgatctcgt cggtcggcgg cacgcgaccc gtgaagtcgc
     2101 cggtcgtcgt catcagcagg ccgacgcgct cgacctcggc gagcagcgcg gcaaaggtgt
     2161 ggcgccggcc ccagatgtcg tgccacgcga ggatccgtgt catcgccgcg ccgatcgtcg
     2221 tccacgggtt cagcgcgccg tcccaggtcc gccacacgcc gtgcaggcgc tcgcgcagcg
     2281 ccggctccgg gtcggccgcc gggccgggcc cgatcgccgc gtcgacgacc tccgccgcca
     2341 gctgccgcgc cagcaccatg cagcgctcgt gctccgcgtc gagcacgacc ctgtcgcccg
     2401 agaggtcggt cagcaggcgg tcgtcggcga tcacgacgac gatcccgggc ggcagccagg
     2461 cgcgcgtctc gtcgcagatc cacacgccgt gccggaccag gcggatcacc ccgggttcat
     2521 ggtgccgcag caagccggcg accccgcggc ggtgcccgtc cgagcccgac tggcagtgct
     2581 gcgcctcgcg cagcgtcagc ccggcggaca cgagctcgcc gttgagcgtg atcggcaccg
     2641 tcgcgtggca gccccgcgcg cgcagccagg tcgcctccgc gagcccgccg cgcaggtggc
     2701 ggacgaagcg gatcatcagc cccggacgaa accgctgctt gacgtggatc tgcgtgccgc
     2761 gggcgtcctt ggccggcgcg acgacatcgg cgacgtcgtg actcgccagc aggctgaccc
     2821 cggtcccgtc cgcgccgctg acgatgcgca cgtgtttggg gttgagcgcc agcgcggcgt
     2881 ggatcccgac cgcgagctgc tggcgtgcgc gttgctcgtc gctatgggcc gccgcgaagc
     2941 tcgcattata aagatcgtcg aggtcctcgc tcgtgatcgc cgcgccgtcg aagcggacga
     3001 tcaggtcgtc gctgtcgcac agcacctcga tcttcgtcgc gcccttgagc gccgcgacgg
     3061 cgatgaagtg caacacgtag cgctgcggct ccgcgacctg gaaggtccgc aacttctcgc
     3121 gcgccttctc gcggtcgaag ctgaagctgc cctcggagtc gacgcgaccg accccctgca
     3181 gcgcggcgat caggtcatcc gttgccatcg cagctccagc gccttcgcgg cgagtgtgtg
     3241 gtcggcctcg atcgagagcc cgcgcccgtg cccgagcacg accagcttgg cgagcacata
     3301 cgccgcgagt tccggctcgc gctccgccag cgcgaccgcc gaggcgaccg ccgggtgcgc
     3361 gacgtggacg atgaggcgct ggccggcgac gagctcgagc cggtcgaggg ccgtcaccgc
     3421 gccgaggtcg tcctgggcga tcgccacgcg cccggccacc gccgagccgg gatagcgcag
     3481 gtcccccgcc gtgatcgccc gcggccgcgg accgacctcg gacagcagcg tcgccagcac
     3541 cggccgcagg cgcgaccagt gcggcgggtc ggcgaccggc agcgacgtgc acagcgccgt
     3601 gtgcagcggc ggcgccggtc ggcccgcgag cagccgcggc agcgcgcaca cccacgcggc
     3661 cgatgcctcg accgtcaccg ccgtgctgtc gtcgccctcc gccgcgatca ccaggtcgcc
     3721 gcgatcgtgc agcgcccgcg tcagcggcga ggcggccgcc gcccagtata cgcgcctcgc
     3781 cttcgccgcc tt
//
LOCUS       DASWNJ010000659         3146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_364349, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000659 DASWNJ010000000
VERSION     DASWNJ010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364349"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VGB85_11015"
     CDS             complement(<1..376)
                     /locus_tag="VGB85_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134607.1"
                     /translation="MRRPLPHARLLALLATLPLLRPGSVLAAPVAAPQELAAFEAGFT
                     EGQAQYDRGEFLEAARTWVRAAERLRETTANRDNRSAVYEYAADAFVRGLAGATDPTA
                     LREAVTALRAYADGFTRAYGTET"
     gene            931..2463
                     /locus_tag="VGB85_11020"
     CDS             931..2463
                     /locus_tag="VGB85_11020"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0134608.1"
                     /translation="MPQEAKADALIGTVLEGRFHVEQLLGEGGMGRVYVADEKRLQRR
                     CALKVLLPELAGDKDCVERFLREAQAIAQIHHENVVDIYHLGDDSGDGVVFFAMELLQ
                     GEDLETRLLDRERRPLSWEQICQWIGQAAGAVAAVHAAGMIHRDLKPSNIFIAKRRDG
                     RQRIKLLDFGIVKTTNRAALTSTGAAIGTPYYMSPEQVLALPLDPRADIYSLGAVLFE
                     ALAGRPVFLGEALQVAMQHCNVPAPRLSAVAPNLTIPPELEALVAQMLAKDPAERPQS
                     MAEIEEFLNSFTPAASPLLSSGSIARPVTRPHSFGSIPSQSLPTVPVAQPTAPTVEAV
                     FDFATPPPARRRLVIPALVGTIVVAAGVVAVLLRGEPETSDPVTPQGPVATVQPVVTP
                     PPEPVVTPVEPGVPSVPVVPTPTPTETDPRPPPDTKLRKPDPKPLDPMKQIAKAAVAC
                     RKQHKAPAKPKMTIDYATGSDGRVTRAVPAINDALGKCFAAAVKATRFPPQLRLGLKT
                     EM"
     gene            complement(2472..>3146)
                     /locus_tag="VGB85_11025"
     CDS             complement(2472..>3146)
                     /locus_tag="VGB85_11025"
                     /inference="COORDINATES: protein motif:HMM:NF025448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shedu anti-phage system protein SduA
                     domain-containing protein"
                     /protein_id="HEY0134609.1"
                     /translation="EVLQRNRGGQFVFKDAENLETGLLTAVAVALADPTYTMSRSVRQ
                     FRAMLDEDRSEDDYRKFIAAHWKVLLGPEYKDCRSPYEVSRGAEVDLFAVRHDDFPDL
                     WELKRPQDPIFRKYNDWYFHSEATAKAFGQMIDYCDLARRDPQVRRGYESRKGLGAVE
                     LSRPRGFVVIGRYTGSAEDRKRQRDRLRLENSYYAGLTLMTYDDLVERAEQFLGFLHG
                     YRPDRD"
BASE COUNT          455 a         1099 c         1146 g          446 t
ORIGIN      
        1 gtgtctcggt gccgtaggcg cgggtgaagc cgtcggcgta cgcacgcagc gcggtcacgg
       61 cctcgcgcag ggcggtcggg tcggtcgcgc cggcgaggcc gcgcacgaag gcgtccgcgg
      121 cgtactcgta gacggccgag cggttgtcgc ggttggcggt cgtctcgcgc aggcgctcgg
      181 cggcgcggac ccaggtgcgc gcggcctcga ggaactcgcc gcggtcgtac tgcgcctgac
      241 cctcggtgaa gccggcctcg aaggcggcga gctcctgggg ggctgccacc ggcgccgcga
      301 ggacagagcc cggccgcagc agcgggagcg ttgcgagcag cgcgaggaga cgggcgtggg
      361 gcaggggacg gcgcatgtcg tgggagggcc ttgggctacg cgggttcgag ggtcgccgca
      421 acgcgatgga tgtgcaactg ccagggacgg tcggtgatgc gagtgtattg cgccgcgatg
      481 tcgggatcgc gtcgcatggg gtaccgcatg aggagggcgc cgcggccgtc gcccggggac
      541 cggccgtgat ctcctcggtg ggtccggtga tcgtcccggc cgcggtggtt tcgctcgcgc
      601 cagtgacatc cccggtcgag gtatccgaat gcagtccgtg gccgtgcccg cgggcccgcc
      661 aggggcggat cgtcccggcg cgcgtcgtct gccagcgccc gcgccagggg cacggccagc
      721 tcgaacgggc gtcgcgtggg ggggcgcatc ggtttgcgga ggcctgcccg cggcaggcga
      781 gcgagtgtat accgccgctg ctggccgcgc agcgcgccgg ggggcagaac cgcccgcccc
      841 tgggcctgga tcgtcacatc gcgggggcgc caggcgacac acttctcgcg tgcaggacat
      901 cggcgcgtgg tgtaggtagc ctgatcgcag atgccacagg aggccaaggc ggacgcgctc
      961 atcgggacag tgctcgaggg ccgcttccac gtcgagcagc tcctgggcga gggcggcatg
     1021 ggtcgcgtct atgtcgcgga cgagaagcga ctgcagcgac gctgcgcgct caaggtcctg
     1081 ctgccggagc tcgcgggcga caaggactgc gtcgagcgct tcctgcgtga ggcccaggcg
     1141 atcgcgcaga tccaccacga gaacgtggtc gacatctatc acctcggcga cgacagcggc
     1201 gacggggtgg tgttcttcgc gatggagctg ctgcaaggcg aggacctcga gacccgcctc
     1261 ctcgaccgcg agcgccggcc gctgtcctgg gagcaaatct gccagtggat cgggcaagcc
     1321 gcgggggccg tcgcggccgt gcacgcggcg ggcatgatcc accgcgacct caagccgtcg
     1381 aacatcttca tcgccaagcg tcgcgacggc cgccagcgga tcaagctgct cgacttcggc
     1441 atcgtcaaga cgaccaaccg cgccgcgctc accagcaccg gcgcggcgat cgggacgccg
     1501 tattacatgt cgccggagca ggtcctcgcg ctgcccctcg acccgcgcgc cgacatctac
     1561 tcgctcggcg cggtcctctt cgaggcgctc gccggccgtc cggtgttcct cggcgaggcg
     1621 ttacaagtcg cgatgcagca ctgcaacgtg ccggcgccgc ggctgtccgc ggtcgccccg
     1681 aacctgacga tcccgccgga gctcgaggcg ctggtcgccc agatgctcgc caaggacccc
     1741 gccgagcgcc cgcagtcgat ggcggagatc gaagagtttc tcaacagctt cacgccggcc
     1801 gccagcccgc tgctcagctc cgggtcgatc gcccgcccgg tgacgcggcc gcactcgttc
     1861 gggtcgatcc cgtcgcagag cctgccgacc gtgcccgtcg cgcagccgac ggcgccgacg
     1921 gtcgaggcgg tgttcgactt cgcgacgcca ccgccggccc gccgccgcct ggtgatcccc
     1981 gcgctggtcg gcacgatcgt cgtcgccgcc ggggtcgtcg cggtcctgct gcgcggtgag
     2041 cccgagacca gcgatcccgt gaccccgcag ggccccgtcg ccaccgtgca gccggtggtc
     2101 acgccgcccc cggagccggt cgtcacgccg gtcgagccgg gtgtgccgag tgtgccagtc
     2161 gtgccgacgc cgacgccgac cgagaccgac ccgcgcccgc ccccggacac caagctgcgc
     2221 aagccggacc ccaagccgct cgacccgatg aagcagatcg ccaaggcggc ggtggcgtgt
     2281 cgcaagcagc acaaggcgcc ggccaagccg aagatgacga tcgactacgc gaccggcagc
     2341 gacggccgcg tgacgcgggc ggtcccggcg atcaacgacg cgctcggcaa gtgcttcgcc
     2401 gccgcggtca aggcgacccg cttccccccg cagctgcggc tcggcctcaa gaccgagatg
     2461 tagccgggct tttagtcgcg gtccgggcgg tacccgtgca aaaagccgag gaactgctcg
     2521 gcgcgctcga cgaggtcgtc gtaggtcatc agcgtgagcc cggcgtagta gctgttctcg
     2581 aggcgcaggc ggtcccgctg gcgcttgcgg tcctcggcgc tgccagtata ccggccgatg
     2641 acgacgaagc cacgaggtcg gctgagctcc acggccccga gccccttgcg cgattcataa
     2701 ccacgccgga cctgggggtc gcgccgcgcg agatcacagt agtcgatcat ctggccgaag
     2761 gccttcgcgg tcgcctcgct gtgaaaatac cagtcgttgt acttgcggaa gatcgggtcc
     2821 tgggggcgct tgagctccca gaggtcagga aagtcgtcat ggcgcacggc gaagaggtcg
     2881 acctcggccc cgcgactgac ctcgtacggc gaccggcaat ccttgtattc gggtccgagc
     2941 aggaccttcc agtgcgcggc gatgaacttg cggtagtcgt cctcgctgcg gtcctcgtcg
     3001 agcatcgcgc gaaactgccg gaccgaccgg ctcatggtgt acgtcggatc ggcgagcgcc
     3061 acggcgaccg cggtcagcag gccggtctcg aggttctcgg cgtccttgaa cacgaactga
     3121 ccgccacggt tgcgctgcaa cacctc
//
LOCUS       DASWNJ010000660         2011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_224413, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000660 DASWNJ010000000
VERSION     DASWNJ010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224413"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..837
                     /locus_tag="VGB85_11030"
     CDS             94..837
                     /locus_tag="VGB85_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134610.1"
                     /translation="MTFIDPHLFITEEGADTDTDTDTDTGGSEPNCVNDVTALVNGLL
                     NSDLAEGKFNLLIRFEDFGVIDEVRLIDAACDPPATEGAQWVCRPDNSPSVNLNTEVI
                     DKSGCRNLEPEVYAPPDLLMINDPGPPCLRTTRGSISLPVSDLVGSLDLREAQVVAML
                     DDAVAPSKLLDGVVYGFLPMATAQDLQINAPLFGVVDLWSVIDAPACMSAYPEYLPNV
                     DEMLINNILVKGAWLAINFTAERVDYRPK"
     gene            complement(868..1086)
                     /locus_tag="VGB85_11035"
     CDS             complement(868..1086)
                     /locus_tag="VGB85_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134611.1"
                     /translation="MDELVKQIVSKTGISETQARSAAETVVGFLKGKLPDAIGGHLDS
                     ALGSAAGALGGVDLGSVTAGLGGLFGKK"
     gene            complement(1280..>2011)
                     /locus_tag="VGB85_11040"
     CDS             complement(1280..>2011)
                     /locus_tag="VGB85_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134612.1"
                     /translation="CSYPDVATSRALAWKNLLFIRHSPTFGGMTGLLYLLVGWNILGS
                     LRGFQIGGFGATLEETLLAALSSASAFTTIVVALLGFFIFTDTHKTWYRFAGGLAHAC
                     AHLGAAIAIGWLMSQVAIALAWPFGGLRHLAATLIALFLGGWIFGALIMGCYLLISIN
                     LFGRHYNEAFSGLCLQDYKQFLRIVVGPDGDLTIYPIGIDRVPRRWREVPDAPRDQAR
                     FVPADDDAQSRPRLIEAPIVVKASA"
BASE COUNT          344 a          662 c          718 g          287 t
ORIGIN      
        1 acgaccggca cgtcgacgac cccgacgacg agcagcgcgt cggagacgac ggccgcgccg
       61 gtcgacacct cgatcacgct gcggatctcg tcgatgacct tcatcgaccc gcacctcttc
      121 atcaccgagg agggcgcgga cacggacacc gatacagaca cggacaccgg gggcagcgag
      181 ccgaactgcg tcaacgacgt cacggcgctg gtcaatggcc tgctcaacag cgacctcgcc
      241 gagggcaagt tcaacctgtt gatccgcttc gaggacttcg gcgtgatcga cgaggtgcgc
      301 ctgatcgacg ccgcatgcga cccgccggcg accgaggggg cgcagtgggt gtgcaggccg
      361 gacaactcgc cctcggtcaa cctcaacacg gaggtgatcg acaagtccgg ctgccgcaac
      421 ctcgagcccg aagtctacgc gccgcccgac ctgctgatga tcaacgaccc cgggccgccg
      481 tgtctgcgga cgacgcgggg atcgatctcc ctgccggtca gcgacctcgt cggatcgctc
      541 gacctgcgcg aggcacaggt ggtcgccatg ctcgacgacg cggtcgcgcc gagcaagctg
      601 ctcgacggcg tggtctacgg gttcctgccg atggcgacgg cgcaggacct ccagatcaac
      661 gcgccgctgt tcggcgtcgt cgacctctgg tcggtgatcg acgccccggc ctgcatgagc
      721 gcctatccgg agtacctgcc gaacgtcgac gagatgctga tcaacaacat cctggtgaag
      781 ggcgcctggc tggcgatcaa cttcaccgcg gagcgagtcg actaccggcc gaagtagtga
      841 gaccgcggcc ggtacccggg gatggggtca cttcttgccg aacagaccgc cgaggccggc
      901 ggtgacgctg ccgaggtcga cgccgccgag cgcgcccgcg gccgagccga gagccgagtc
      961 gaggtggccg ccgatggcgt cggggagctt gcccttgagg aagccgacga cggtctccgc
     1021 ggcgctgcgc gcctgggtct cggagatgcc ggtcttgctt acgatctgct tgacgagttc
     1081 atccatggtg ttgactcctg agcgcggcag tatcggggtg acaggcggcg cgcgtcaatc
     1141 gtggcccggg cgcgcggcca ggccagggtc ggcgcgccgc cgggacatgg ccggcatcgg
     1201 cgcgaatttc cgtcgaattt ccgtcgatct tccggcgcga ggccgaagcg ctgcgccgac
     1261 ctgcgcaggc aacgcggggc tacgcgctgg ctttgacgac gatcggggcc tcgatcaggc
     1321 gcggtctgct ttgggcgtcg tcgtctgcgg gcacgaagcg ggcctggtcg cgcggggcat
     1381 cgggcacctc gcgccagcgc cggggcacgc ggtcgatgcc gatcgggtag atcgtcaggt
     1441 cgccgtcggg gccgacgacg atccgcagga actgcttgta gtcctgcagg cacaggccgc
     1501 taaacgcctc gttgtagtgg cggccgaaca ggttgatcga gatcagcaga taacagccca
     1561 tgatcagcgc gccgaagatc cagccgccga ggaacagggc gatcaaggtc gcggcgaggt
     1621 gacgcaggcc gccaaacggc cacgccagtg cgatcgccac ctggctcatc agccagccga
     1681 tcgcgatcgc ggcgccgagg tgagcgcacg cgtgggccag gccgccggcg aagcggtacc
     1741 aggtcttgtg cgtgtcggtg aagatgaaga agccgagcag cgcgacgacg atcgtggtga
     1801 aggcggaggc cgagctcagg gccgcgagca gggtctcctc gagggtcgcc ccgaagccgc
     1861 cgatctggaa cccgcgcagg ctgccgagga tgttccagcc gaccagcaga tacagcaggc
     1921 cggtcatgcc gccgaaggtc gggctgtggc ggatgaacag caggttcttc cacgcgagcg
     1981 cccgcgaggt cgcgacgtcg gggtagctgc a
//
LOCUS       DASWNJ010000661         1985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_47676, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000661 DASWNJ010000000
VERSION     DASWNJ010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47676"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..1504
                     /locus_tag="VGB85_11045"
     CDS             206..1504
                     /locus_tag="VGB85_11045"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEY0134613.1"
                     /translation="MTKGRRFFKLAGMTAQVAGSYATTRIKSMFQSEENAETDLKAAH
                     ERNGGRIAKTLGELKGAVMKVGQMASIAGDILPKELAEALRGLQREAPPVDFEVIAGQ
                     IERELGAPPESLFKRFDRKPFASASIGQVHRATTDDGREVVVKVQYPGVDTAVSSDLS
                     QLKLVLKASGLIKIRKQALDEVFKEVKARLAEELDYCNEADNVRKFREYHARHPFVVI
                     PEVVGERSAQRVLTLTYEPGDHVSDLDKLGYDQPTRDLLGQHLFKLLIDQLFEFRNLH
                     ADPNPGNLAFRRDGSVVLYDFGCVKQIPDAIIEAYGKTIRCGIEEDYVGVEEGLRGLG
                     VLNPDGPPVEHAYYKIFRDIFALPFLANEVFDYGRSTIHDEVLKQVPGAIKRMESFQP
                     PSELVFIDRAIAGHYGNLRKIGARGRFLAMLWPYLYPESA"
     gene            complement(1506..>1985)
                     /locus_tag="VGB85_11050"
     CDS             complement(1506..>1985)
                     /locus_tag="VGB85_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134614.1"
                     /translation="QLGFDKEALQYLGEDGVWLKIPEASAAEVRLEIAKAVRGLDFVA
                     DAFTSDELAGPTDPARPFHAEYQRSYYAGRSPDIQMRFKQWHLVDAQTRGTSHGSPYE
                     YDTHVPVLFFGAGVQPGIQKQRVGTTDVAPTLAELLGLTAPAGTDGRSLARVVRGPR"
BASE COUNT          338 a          696 c          651 g          300 t
ORIGIN      
        1 cgcgggagat cgtgaagcaa cgcgcagcaa cgccggactg atcaccccgg agtgatcacc
       61 cacgactgat cgcccacgac aatcgcgcca gagcgtcaca gcgcgccaca tcgtcattac
      121 gcggccccgg cgctgccttg cgccgcgagg gccccggggg ccatagtgag cccgccaggc
      181 ggggggcgtg atgaccgaag gaccggtgac gaaggggagg cgcttcttta aactcgcggg
      241 catgaccgcc caggtcgcgg gcagctacgc gacgacgcgg atcaagtcga tgttccagtc
      301 ggaggagaac gccgagaccg acctcaaagc cgcccacgag cgcaacggcg gccggatcgc
      361 aaagaccctc ggcgagctca agggcgccgt gatgaaggtc ggccagatgg cctcgatcgc
      421 cggcgacatc ttgccgaagg agctggccga ggcgctgcgc ggtctgcaac gcgaggcccc
      481 gccggtcgac ttcgaggtga tcgccggcca gatcgagcgc gagctcggcg cgccgccgga
      541 gtcacttttt aaacgcttcg accgcaagcc gttcgcgtcc gcgtcgatcg gccaggtcca
      601 ccgcgcgacc accgacgacg gccgcgaggt cgtggtcaag gtccagtacc ccggggtcga
      661 cacggcggtc agctcggatc tctcgcagct caagctcgtg ctcaaggcga gtggcctgat
      721 caagatccgc aagcaagcgc tcgacgaagt ttttaaagag gtcaaggctc gcctcgcgga
      781 ggagctcgac tactgcaacg aggccgacaa cgtccgcaag ttccgcgagt accacgcgcg
      841 ccaccccttc gtcgtcatcc cggaggtcgt cggcgagcgc tctgcccagc gcgtgctgac
      901 cctgacctac gagcccggcg accacgtctc cgacctcgac aagctcggct acgaccagcc
      961 gacgcgcgac ctgctcggcc agcacctctt caaacttctc atcgaccagc tgttcgagtt
     1021 tcgcaacctc cacgccgacc cgaacccggg caacctcgcg tttcgccgcg acggcagcgt
     1081 cgttctctac gacttcggct gcgtcaagca gatccccgac gcgatcatcg aggcttacgg
     1141 caagacgatc cgctgcggca tcgaggagga ctacgtcggc gtcgaggagg gcctgcgcgg
     1201 cctcggcgtg ctcaaccccg acggcccgcc cgtcgagcac gcctattata agatcttccg
     1261 cgacatcttc gccctcccgt tcctcgcgaa cgaggtcttc gactacggcc gctcgacgat
     1321 ccacgacgag gtcctcaagc aggtccccgg cgccatcaag cgcatggagt cgttccagcc
     1381 cccgtccgag ctggtgttca tcgaccgcgc gatcgccggg cactacggaa atttgcggaa
     1441 gatcggcgcg cgcgggcgct tcctcgcgat gctctggccg tatctctatc cggagtccgc
     1501 ctgagctacc gcggtccacg gacgacccgc gccaggctgc gcccatcggt gccggccggc
     1561 gcggtgagcc cgagcagctc ggcgagcgtc ggcgcgacgt cggtcgtgcc cacccgctgc
     1621 ttctgaatac cgggctgcac gccggccccg aagaacagca ccggcacgtg cgtgtcgtac
     1681 tcgtagggcg agccgtggct ggtgccgcgg gtctgcgcgt cgacgaggtg ccattgtttg
     1741 aagcgcatct ggatgtcggg gctgcggccg gcgtagtaac tgcgctggta ctcggcgtgg
     1801 aacgggcgcg cggggtcggt ggggccggcg agctcgtcgg aggtgaaggc gtcggcgacg
     1861 aagtcgaggc cgcgcactgc cttcgcgatc tccaggcgca cctcggcggc gctggcctcg
     1921 gggatcttca gccacacgcc atcctcgccg aggtactgca gggcctcctt atcaaacccg
     1981 agctg
//
LOCUS       DASWNJ010000662         2048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_471187, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000662 DASWNJ010000000
VERSION     DASWNJ010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471187"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VGB85_11055"
     CDS             <1..641
                     /locus_tag="VGB85_11055"
                     /inference="COORDINATES: protein motif:HMM:NF024731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0134615.1"
                     /translation="LFLDELPEFSRGCLEGLREPLEDKLVHIVRVRGALRLPANFQLM
                     AAMNPCPCGYLGHPERSCVDPASAVQRYQQRLSGPLLDRIDLVVPVMPTTAEELGRRK
                     PGERSAQIRERVATARAVARRRLAGTPWRSNADIPATDGVIERLCELTPAAERLLLSL
                     AKARALSPRALHRLRRVARTIADLGGGDPSAPVGELQVAEAAQLRRLPELCE"
     gene            complement(705..1244)
                     /locus_tag="VGB85_11060"
     CDS             complement(705..1244)
                     /locus_tag="VGB85_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134616.1"
                     /translation="MAFKVGEDIDAWCTKCRTDTLHVVVALKPGVPEPKRVECKECHG
                     QHMYHAPKIGPAQPAATGPAVTNRTAAARTPKAPKVPVAPKVAAAAKAARALKSAAAT
                     AHTLGAREWIKRMDEFALQHDLVMRSYSMRETFSPGEPVQHPKFGKGVVVEIVDKNKV
                     AILFEEGRKVLAMGAIPSA"
     gene            1522..>2048
                     /locus_tag="VGB85_11065"
     CDS             1522..>2048
                     /locus_tag="VGB85_11065"
                     /inference="COORDINATES: protein motif:HMM:NF019626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine/phenylalanine carboxypeptidase
                     domain-containing protein"
                     /protein_id="HEY0134617.1"
                     /translation="MDAENLERIRVADGRLVAAAKDVKVLSQLAWPARLLDEFLRHWK
                     ARNPRLPDPPRGGAQFTENCDELRAVMADCDLDEPIGRYLYRTAHSYVTAARMVERIG
                     TPEFTQRSIALYGQPHDRIGPNAVTNLEAAEQFLSLTDDFRQVADPAEEVCLRPEYVA
                     ERLSERLAPIFVAHP"
BASE COUNT          299 a          682 c          783 g          284 t
ORIGIN      
        1 tgctgttcct ggacgagctg cccgaattct cgcgcggctg cctcgagggt ctgcgtgagc
       61 cgctcgagga caagctggtc cacatcgtgc gggtccgcgg ggcgctgcgg ctgccggcga
      121 attttcagct gatggcggcg atgaacccgt gcccctgcgg ctacctcggg cacccggagc
      181 gcagctgcgt cgacccggcg agcgcggtgc agcgctatca gcagcggctg tcggggccgc
      241 tgctcgaccg catcgacctc gtcgtgccgg tgatgccgac gaccgcggag gagctcgggc
      301 gccgcaagcc cggggagcgc agcgcacaga tccgcgagcg cgtggcgacg gcgcgggcgg
      361 tcgcgcggcg gcgcctcgcg ggcacgccgt ggcgcagcaa cgccgacatc cctgcgaccg
      421 acggcgtgat cgagcggctg tgcgagctga cgcccgcggc cgagcgcctg ctgctgtcgc
      481 tggcgaaggc gcgggcgttg agcccacgcg cgttgcaccg cctgcgccgg gtcgcgcgga
      541 cgatcgccga cctcggtggc ggagacccga gcgcgccggt cggcgagctc caggtcgccg
      601 aggcggccca gctgcgccgg ctaccggagc tgtgcgagtg aggcctcagg cccggagggc
      661 cgtgaagcca gtcaggcccg tcagggccgg ggaggccacc cgcgtcaggc gctggggatg
      721 gcacccatgg cgaggacctt gcggccctcc tcgaacaaga tggcgacctt gttcttgtcg
      781 acgatctcga cgacgacgcc cttgccgaac ttcgggtgct ggacgggctc accgggggag
      841 aaggtctcgc gcatcgagta cgagcgcatg acgaggtcat gctgcaaggc gaactcgtcc
      901 atgcgcttga tccactcgcg ggcgccgagg gtgtgcgcgg tcgcggcggc gctcttgagg
      961 gcgcgggcgg ccttggcagc ggcggcgacc ttcggagcga ccgggacctt gggcgccttg
     1021 ggggtgcgcg cagcggcggt ccggttggtc acggcagggc cggtggcggc gggctgggcc
     1081 ggaccgatct tcggcgcatg gtacatgtgc tgaccgtggc actccttgca ctcgacgcgc
     1141 ttgggttcgg gcacgccggg cttgagcgcg acgacgacgt gaagggtatc ggtgcgacac
     1201 ttggtgcacc acgcgtcgat gtcctcgcca actttgaagg ccatgagcaa ggggcagaca
     1261 atacggcaaa catatcgccg ctgccaacag gcgcgacgcg cggacagctg tcctgtgaag
     1321 ggccataaaa cgcacgattc gcgggcgaag cggcgccggg acggagcact tcgaggccgg
     1381 tgaaacggcc gtgtacgtgc cacagcgcgc caacctcggc gcgtgcaggc catcggcggc
     1441 cgggcgcgcg cagccacggg gccgcgggcg cgcgtcgggg tttttttacg cgatggccgt
     1501 cgacgcgtaa gatggtggag aatggacgcc gaaaacctcg agcggatcag ggtggccgac
     1561 gggcgtctgg tcgcggcggc caaggacgtc aaggtgctca gccagctcgc gtggccggcg
     1621 cggctgctcg acgagttcct gcgccactgg aaggcccgca acccgcggct gccggatcct
     1681 ccgcggggag gggcccagtt caccgagaac tgcgacgagc tgcgggccgt gatggccgac
     1741 tgcgacctcg acgagccgat cgggcgctac ctctaccgga cggcgcacag ctacgtgacc
     1801 gcggcgcgga tggtcgagcg gatcgggacc cccgagttca cgcagcggtc gatcgcgctg
     1861 tacgggcagc cgcacgaccg catcgggccc aacgcggtga ccaacctcga ggcggccgag
     1921 cagttcctgt cgctcaccga tgacttccgc caggtcgcgg acccggccga ggaggtctgc
     1981 ctgcgcccgg agtacgtggc ggagcggctg tcggagcgac tggcccccat cttcgtggcc
     2041 cacccgat
//
LOCUS       DASWNJ010000663        10533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_505790, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000663 DASWNJ010000000
VERSION     DASWNJ010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 10533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10533
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505790"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VGB85_11070"
     CDS             <1..682
                     /locus_tag="VGB85_11070"
                     /inference="COORDINATES: protein motif:HMM:NF025670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134618.1"
                     /translation="VINGDKESNTASSAGAVYVFERTGKDWSNPAYIKPTNTGAGDWF
                     GRSVAMSADGGTLAVGAPNERSDATGVGAQQSDNSIEEAGAVYMFVRTDKWSQQEYIK
                     ASNTEAYDNFGQSVALSADGDMLAVGAAGEDSSAAGIAGSQTDNFAAAAGAVYMFTRA
                     LDVWSQKAYVKAPNAAQTDYFGHSVAVSGDGDVLAVGAFGEESTAIRIGGDQKNNTAP
                     DAGAVYFY"
     gene            complement(730..1530)
                     /locus_tag="VGB85_11075"
     CDS             complement(730..1530)
                     /locus_tag="VGB85_11075"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0134619.1"
                     /translation="MLRSLALSLSVLLACAAEAPKPEAAQAEPAKPEPVEAEPEKPAK
                     PAPDAKEACAKIVVVAWAGAEGAEATVTRDEAAAKTTAEALREKLVGGAAFAEVAAQS
                     DEPKTRAKGGAMGTFERDKWPEKYAAIADAVFGLQVGETSPAIRTPLGHVVAQRCAVE
                     KIHSRHILVRYKGAKNAEASIKRDAKAARALAEKVHAEATAAGADFAVIARKYSEDGS
                     ADRGGDLGSVGRGMFAPAFETAAFALKPGETSAVVETDFGFHVIQRVE"
     gene            complement(1550..2323)
                     /locus_tag="VGB85_11080"
     CDS             complement(1550..2323)
                     /locus_tag="VGB85_11080"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEY0134620.1"
                     /translation="MTHPDLALAHCTYPEVAARMADARPKVAIIPLGSTEAHGPHLPL
                     NTDSLIGAAVAERAAAALHARGLVALHFPPIHYAVTDWAGEFAGSVSLAMGTAIDLVL
                     GTCRAARAMGFDEVVIFTAHLEPGHIASLREVVRRYEGEAGKPLVFPDTTRRALATRL
                     TPEYQSGSCHAGQYETSLILALRPDLVRWEVARALPVHDVPLAERIKDGARDFVECGM
                     DRAYCGAPAAATAEEGHQSLAVLTELVVEAVLAAVAVKR"
     gene            complement(2320..3903)
                     /locus_tag="VGB85_11085"
     CDS             complement(2320..3903)
                     /locus_tag="VGB85_11085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEY0134621.1"
                     /translation="MSAEPIEIPDRLNMAEWFLDARLAEGLGDKTAIYYGEAQVTYRE
                     LVADSCRVTNLLRSLGLRIEDRVQLLLLDTPEFVAAYFGILRAGCVAVPTNTWLLDKD
                     YAYYLEYARPRAVIIDAEVYPQFAEVWRDAAYPPKVIVVDRSGGRAPEGTIDWHAEMA
                     RQSPEAETERTYRDDFSTWLSSSGSTGRPKCVVHMHHDFVWNTIAYAQRTLGIKRDDI
                     TMSAPKLFFGYALASNMLFPFSVGASCVLLPERTPAEQYYALIERYKVTQLVTVPTTI
                     AKMADHSELAAKHDLGSLHSLVSAGEALPARIYRTWKQATGVEIYDGIGSAEMFHVYI
                     TNRPGDVKEGSLGRIVEGYTHRLIDDDGRDVMPGEIGTLVITGPSAGQGYWRMRDLSR
                     ATFWGDSVRGADKFHVDTDGYFWFCGRGDDMLKCSGVYVSPVEVENCLLGHPAVHEAG
                     VVGYRDGAGLEKAIAYVELAPGHQPSDSLATEILAHARGNIAAFKAPRRIEFIAALPR
                     TETGKIRRGELRRLAEELT"
     gene            4037..4534
                     /locus_tag="VGB85_11090"
     CDS             4037..4534
                     /locus_tag="VGB85_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134622.1"
                     /translation="MQLVYPRLGVELLRDSFVVWVRWVSNEQPPSPGEPPAQGVRVEL
                     LLNRNSVLGPTIVYRRELDAPVFLRANRLRVGEVLRSAGSGTGRLDIQLVIHGSIAHA
                     PWAALYLVRDDEGDAIGGGELPGTPLLQLQPSTPADRWHVAAQANLRVQLELEGERRH
                     LTVLR"
     gene            4596..5435
                     /locus_tag="VGB85_11095"
     CDS             4596..5435
                     /locus_tag="VGB85_11095"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0134623.1"
                     /translation="MQRVTGAFKERLASNVVASAQRIRAAGVPFYMALPEPVIVGLLT
                     RAFEAAARDLEAGGTSNLVRLMAGLGAERSKQGIAVIDVLNGLNIGFQVVSDDFAALF
                     ADDLEARIAWESSRSQLAFAGAASLATEYLTAREAVVRAQAEEIAELSLRVLPLYPGV
                     LVLPLVGRISGERAGQITQVLLAEIARHRCRFALLDLSGVATFDAEVAANLLRAARAV
                     QLLGATPILVGLTAVAARTIAGLGGEAGAALGPLLTLADLESGLRHAMTHLGITWGPQ
                     ASR"
     gene            5555..6781
                     /locus_tag="VGB85_11100"
     CDS             5555..6781
                     /locus_tag="VGB85_11100"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0134624.1"
                     /translation="MRSSSLLSLCLFASACSVEDDATSDPPGTATQGPTGAAGSTSDA
                     PGTTSSTDATTTTGEATSTGLSTTEPTTEPTTAMTATTATSEPGYCGNGVLDEYESCD
                     DGEYNADDAACTSSCRFAVCGDGLVHAGFEACDDGNTYNTDACLNGCIVASCGDGFRG
                     PGEACDDGNAVDDDECPNSCSGGGCGDGFVQVGEECDDGNVEYDDACLNTCELATCGD
                     GFTQVGVEECDDANQTDSDTCSNSCKKPTCSDGKRNGFETDVDCGGVYCDGCLLGEKC
                     NGNLDCGDGICKANECVPPLPLDPPNCAGATVGVDQAYGAIKQSCGCHGGGAGGLTFT
                     NASDFRNNMVGVNSQTAAMKIVTAGDINQSYLIYKILNQHSKVVGGRGNQMPLGKSLS
                     DNQKCLLINWIKSGAK"
     gene            complement(6866..7684)
                     /locus_tag="VGB85_11105"
     CDS             complement(6866..7684)
                     /locus_tag="VGB85_11105"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0134625.1"
                     /translation="MRSIVVALLLSTLALSGCKRKELEAALAEANKKLEATNGELGAE
                     RGKTKQLTEENQTLQQKIASLEADITRLSGEIKVLEEQNADLAKKAGATAEELKALRA
                     EKQKRNAELQVYKDLFGKLKALVDAGTIQVEFRKGRLVVKLASSILFDSGKTELKPEG
                     QTALANLASALKSLNDREFLVAGHTDNVPIKTARFKNNWDLSTARAVEVVQYLISQGM
                     TSKNIGAAGFGEFDPITDNKTEDDKAKNRRIEVILMPLLGDIPGMQEMLTGGKS"
     gene            7875..>10533
                     /locus_tag="VGB85_11110"
     CDS             7875..>10533
                     /locus_tag="VGB85_11110"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEY0134626.1"
                     /translation="MPRPWGEFLGIVLLAIGVLMAGGLCSYQFGTGYLMGPVGHLAAS
                     ALYATLGMGAYLLVLGVLGLGVKALLGETMEIRAGEGLGFAGATIAGCVLLHVTFPEY
                     RTHGLTSGGLTGELLGEVGLGLFDRAGTYLIAFALLFVGLFASTPLSVEHLVIGVRLA
                     VRGTIAVGAYLWGGLVGLYDALRGRPDEHSEQDEEEEYEEDAEEEDELDEEAEEEEEE
                     EEEPAPPPKPRRSRKAAAPAEPVALPAPAVAPAAMTEPAAEPRKNRKLPRRPEDMPEI
                     VVAGPTRPEEAAKPEPKKKPKAEPVVEEAEDESADAVEPAANSGAPKKRRKAPAPEIV
                     EPEDEDDASVADEREAPAEEPAPVSRTGKKPPPRPAEIMPPAAVTPAPVQLPLAEAAK
                     PPERTPAIPGIVRLTAGPFLLPPSKLFELPEQAVTEVDKGFVLEQAERIEHALSTFKV
                     TGKVTKIHPGPVITRYEFKPDPGMKLSRIESLENDLAMALEAISIRILAPIPGKATVG
                     FEVPNVVRETVAIKEILDSEGFGPERTFKLPMALGKNITGKPITIDLAKAPHLLVAGA
                     TGSGKSVGVNAMICSLLYSCTPEDVRMIMIDPKMLELSIYKDIPHLLLPVITDAEAAN
                     LALRWAVDEMERRYSVLSEAQVRDIGGYNKKLPILLEQFESEKKMLEMEADRANLDSE
                     EEGEDAVATEFLGGVAFDADGHPMRQLGSDMHGAKLGDRPEKMPYIVVIIDEFADLMM
                     VASKEVETSVARIAAKARACGIHLILATQRPSVDVITGTIKNNFPSRIAFQVTSDIDS
                     RTILDGKGAKQLLGMGDMLYMDRGAQPQRVHGCYVSEEEIFKVVAFIKKQARPVYNHA
                     IAAKREEDLEEEPSDRRADPLYDKAVQIVA"
BASE COUNT         1604 a         3685 c         3679 g         1565 t
ORIGIN      
        1 tgtcatcaac ggcgacaagg agtccaacac ggcgtcctcc gctggcgccg tatatgtctt
       61 cgagcgcacg ggcaaggact ggtcgaatcc agcatatatc aagcccacga acaccggggc
      121 aggggactgg ttcggccgca gcgtggccat gtcggcggat ggtggcaccc tcgctgtcgg
      181 cgcccccaac gagaggagcg acgccactgg tgtcggcgcc cagcagagcg acaattcaat
      241 cgaggaggcc ggcgccgtat atatgttcgt gcgcacggac aagtggtcac agcaggagta
      301 catcaaggcc tccaatacgg aggcgtacga caacttcgga cagagcgtcg cgctgtcggc
      361 cgacggggat atgctcgccg tcggtgcggc cggtgaagac agctccgcgg ccggtatcgc
      421 cggcagccag accgacaact tcgccgcggc cgccggcgcg gtctacatgt tcacgcgtgc
      481 gctcgatgtc tggtcgcaga aggcctacgt caaggctccc aacgccgcgc agaccgacta
      541 ctttgggcac agcgtcgcgg tgtcgggcga cggtgatgtg ctcgccgtcg gagcattcgg
      601 cgaggagagc acggcgatcc ggatcggcgg cgaccagaag aacaacacgg ccccggacgc
      661 cggtgcggtt tatttttatt gaccgcgagc cccgcgtctg acctccgctg agctgcccca
      721 cgggacagct cactcgacgc gctggatgac gtgaaagccg aagtcggtct cgacgacggc
      781 gctcgtctca cccggcttga gcgcaaaggc ggcagtttcg aaggccggcg cgaacatgcc
      841 gcgaccgacg ctgccgaggt caccgccgcg atcggccgag ccgtcctcgc tgtacttcct
      901 ggcgatcacc gcgaagtcgg ccccggccgc ggtcgcctcc gcgtggacct tctcggccag
      961 agcgcgcgcc gccttcgcgt cgcgcttgat gctcgcctcg gcgttcttgg cccccttgta
     1021 gcggaccagg atgtgccgcg agtggatctt ctcgaccgcg caacgctgcg cgacgacatg
     1081 gccgagcggc gtgcggatcg ccgggctcgt ctcgccgacc tgcaggccga acaccgcgtc
     1141 ggcgatcgcc gcgtacttct ccggccactt gtcgcgctcg aaggtcccca tcgccccgcc
     1201 cttggcccgc gtcttcggct cgtcgctctg cgccgcgact tcggcgaagg cggccccgcc
     1261 gacgagcttc tcgcgcagcg cctcggcggt ggtcttcgcg gccgcctcgt cccgcgtcac
     1321 cgtcgcctcg gccccctcgg cgccggccca cgcgaccacc acgatcttcg cgcacgcctc
     1381 cttcgcgtcc ggggccggct tggcgggctt ctccggctcg gcctcgaccg gctcgggctt
     1441 cgccggctcg gcctgcgccg cttcgggctt cggcgcctcg gcggcgcagg cgagcagcac
     1501 ggacagggac agcgcgagcg agcggagcat gcccgcaggg taccgcaggt cagcgcttga
     1561 ccgcgaccgc ggcgagcacg gcctcgacga cgagctccgt cagcaccgcg agcgactgat
     1621 ggccctcctc ggcggtcgcg gccgccggag cgccgcagta ggcccggtcc atgccgcact
     1681 cgacgaagtc gcgggcgccg tccttgatcc gctcggcgag cggcacgtcg tgcacgggca
     1741 gcgcgcgggc gacctcccag cggacgagat ccggccgcag cgcgaggatc aggctcgtct
     1801 cgtactgacc ggcgtgacac gagccgctct ggtactccgg cgtcagccgg gtcgccagcg
     1861 cgcgccgggt cgtgtccggg aacaccagcg gcttgccggc ctcgccctcg tagcgcctca
     1921 ccacctcgcg caagctggcg atgtgcccgg gctcgaggtg cgccgtgaag atcacgacct
     1981 catcaaaacc catcgcccgc gccgcgcgac atgtcccgag gaccaggtcg atcgccgtgc
     2041 ccatcgccag cgacaccgag cccgcgaact cgccggccca gtcggtcacc gcgtagtgga
     2101 tcggcgggaa gtgcagggcg accaggccgc gggcgtgcag cgccgccgcg gcccgctcgg
     2161 ccaccgcggc gccgatcagg ctatcggtgt tgagcggcag gtgcgggccg tgggcctccg
     2221 tgctgccgag cgggatgatc gccaccttcg ggcgcgcgtc ggccatgcgc gcggcgacct
     2281 cgggataagt gcagtgcgcg agcgcgaggt cggggtgcgt caagtgagct cctcggcgag
     2341 cctgcgcagc tcgccgcggc ggatcttgcc ggtttctgtg cgcggcaggg ccgcgatgaa
     2401 ctcgatgcgg cgcggcgcct tgaacgccgc gatgttcccg cgcgcgtgcg ccaggatctc
     2461 cgtcgccagc gagtcgctcg gctggtggcc cggcgccagc tcgacgtagg cgatcgcctt
     2521 ctcgagcccg gccccgtcgc ggtaaccgac caccccggcc tcgtgcacgg ccgggtggcc
     2581 gagcaggcag ttctcgacct cgaccggcga cacgtacacg cccgagcact tgagcatgtc
     2641 gtcgccgcgc ccgcagaacc agaagtagcc gtcggtgtcg acgtggaact tgtcggcgcc
     2701 gcgcaccgag tcgccccaga aggtcgcgcg gctgaggtcg cgcatgcgcc agtagccctg
     2761 gcccgccgac ggcccggtga tcaccagcgt gccgatctcg ccgggcatga cgtcgcggcc
     2821 gtcgtcgtcg atcaggcggt gcgtgtagcc ctcgacgatc ctgccgaggc tgccctcctt
     2881 gacgtcgccg gggcggttgg tgatgtacac gtggaacatc tccgccgagc cgatgccgtc
     2941 gtagatctcg acgccggtcg cctgcttcca ggtccggtag atgcgggccg gcagcgcctc
     3001 gcccgcgctg accagcgagt gcaggctgcc gaggtcgtgc ttcgccgcca gctcactgtg
     3061 atcggccatc ttggcgatcg tcgtcggcac ggtgaccagc tgcgtcacct tgtagcgttc
     3121 aatcaaggcg taatactgct ccgccggcgt gcgctcgggc agcagcacgc agctggcccc
     3181 gacgctgaac gggaacagca tgttgctcgc cagcgcgtag ccgaagaaca gcttgggcgc
     3241 cgacatcgtg atgtcgtcgc gcttgatgcc gagcgtgcgc tgggcgtacg cgatcgtgtt
     3301 ccacacgaag tcatggtgca tgtgcacgac gcacttcggt cgcccggtcg agccgctcga
     3361 gctcagccag gtcgagaagt cgtcgcggta ggtccgctcg gtctccgcct cggggctctg
     3421 gcgcgccatc tcggcgtgcc agtcgatcgt gccctcgggc gcccggccgc cgctgcggtc
     3481 gacgacgatc accttcgggg ggtacgccgc gtcgcgccag acctccgcga attgcgggta
     3541 cacctcggcg tcgatgatca ccgcgcgcgg gcgcgcgtac tcgagataat acgcgtagtc
     3601 cttgtcgagc agccaggtgt tggtcggcac cgccacgcag ccggcgcgaa ggatcccgaa
     3661 gtaggcggcg acgaactccg gcgtgtcgag cagcaacagc tgcacgcggt cctcgatccg
     3721 caggccgagg ctgcgcagca agttcgtcac ccggcacgag tccgcgacga gctcgcggta
     3781 ggtcacctgg gcctcgccgt agtagatcgc ggtcttgtcg ccgagcccct ccgcgaggcg
     3841 ggcgtccaaa aaccactcgg ccatgttgag gcggtcgggg atctcgatcg gttcggccga
     3901 catcgtcgtc acggtcgcac gcctgcgagc gcgcatcgcg tcatggcaca ggtgcgggcg
     3961 ctcacacgct cccggccggc accacgggcc ctggacgggg gtgacgggcc tcggcccgcg
     4021 cgcgtacagt cggcgcgtgc agctcgtgta cccgcggctc ggcgtggagc tcctgcgaga
     4081 cagcttcgtc gtgtgggtcc gctgggtctc gaacgagcag ccgccctcgc ccggcgagcc
     4141 gcccgcgcag ggcgtccgcg tcgagctgct gctcaaccgc aacagcgtgc tcggtccgac
     4201 gatcgtgtat cgccgcgagc tcgacgcccc ggtgttcctg cgggccaacc gcctgcgcgt
     4261 cggcgaggtc ctgcgcagcg ccggcagcgg gaccggtcgc ctcgacatcc agctcgtcat
     4321 ccacgggtcg atcgcccacg cgccgtgggc cgcgctgtac ctcgtgcgcg acgacgaggg
     4381 cgacgcgatc ggcggcggcg agctgcccgg caccccgctc ctgcaactgc aaccttcgac
     4441 ccccgccgac cgctggcacg tcgccgccca ggccaacctt cgcgtgcagc tcgagctcga
     4501 gggcgagcgc agacacctga ccgtgctgcg ctgacgcgcg aggtcgcccg cgcggccgcg
     4561 gcgttcggcg tggcgatggg tgttagtctc gcggcatgca gcgcgtgacc ggggccttca
     4621 aggagcgcct cgccagcaac gtggtcgcct cggcgcagcg catccgcgcc gcgggggtac
     4681 cgttttacat ggccttgccg gagcctgtga tcgtcggtct gctgacgcgg gccttcgagg
     4741 ccgcggcgcg ggacctcgag gccggcggga ccagcaacct cgtgcgcttg atggccggac
     4801 tcggggccga gcggtcgaag cagggcatcg ccgtcatcga cgtgctcaac ggcctcaaca
     4861 tcggctttca ggtcgtcagc gacgacttcg cggcgctgtt cgccgacgac ctcgaggccc
     4921 ggatcgcgtg ggagtcgtcg cgcagccagc tcgcgttcgc gggcgcggcc tcgctggcga
     4981 ccgagtacct gacggcgcgt gaggccgtgg tccgcgcgca ggccgaggag atcgccgagc
     5041 tgtcgctgcg ggtgttgccg ctgtaccccg gcgtgctcgt gctgccgttg gtgggccgga
     5101 tctccggcga gcgcgccggc cagatcaccc aggtgttgct cgcggagatc gcacgccatc
     5161 gctgtcgctt cgcgctgctc gacctctccg gcgtcgccac cttcgacgcc gaggtcgcgg
     5221 ccaacttgct ccgcgccgcg cgggcggtgc agctgctcgg cgcgaccccg atcctcgtcg
     5281 gcctgaccgc ggtcgcggcg cggacgatcg cgggtctcgg cggtgaggcg ggcgcggctc
     5341 tcggaccgct gctgacgctc gccgacctcg agagcgggct gcgccacgcg atgacgcacc
     5401 tcggcatcac gtgggggccg caggcgtcgc gctgattcac cgatctcgcg gtccgtcgtc
     5461 gtgtcgcgct ccgcgtgcgc gcgcgcgatc gggcggccat ccacccttgg cggcccgggc
     5521 gtcggcaggc actctcgctg tcccgaaaga tctcatgcgc tcgtcctccc tcctgtccct
     5581 gtgtctgttc gcgtcggcct gcagcgtcga ggacgacgcg acgagcgacc cgcccggcac
     5641 cgccacgcag gggccgaccg gcgccgccgg ctcgacgagc gacgcgcccg gcacgacctc
     5701 gagcaccgac gcgacgacga cgacgggcga ggcgacgagc accgggctga gcaccaccga
     5761 gcctaccacc gagccgacca cggcgatgac cgcgacgacc gcgacgagcg agcccggcta
     5821 ctgcggcaac ggtgtgctcg acgagtacga gtcctgcgac gacggcgagt acaacgccga
     5881 cgacgccgcc tgcacgagct cgtgtcgctt cgccgtgtgc ggtgacggcc tggtccacgc
     5941 cggcttcgag gcctgcgacg acggcaacac gtataatacg gacgcgtgcc tcaacggctg
     6001 catcgtcgcc agctgcggcg acggctttcg cgggccgggc gaggcgtgcg acgacggcaa
     6061 cgcggtcgac gacgacgagt gccccaacag ctgcagcggc ggcggctgcg gcgacggctt
     6121 cgtgcaggtc ggcgaggagt gcgacgacgg caacgtcgag tacgacgacg cctgcctcaa
     6181 cacctgcgag ctggcgacct gcggcgacgg cttcacgcag gtcggcgtcg aggagtgcga
     6241 cgacgccaac cagaccgaca gcgacacgtg cagcaacagc tgcaaaaagc cgacctgcag
     6301 cgacggcaag cgcaacggct tcgagaccga cgtcgactgc ggcggcgtct actgcgacgg
     6361 ctgtctgctc ggcgagaagt gcaacggcaa cctcgactgc ggcgacggga tctgcaaggc
     6421 caacgagtgc gtgccgccgc tgccgctgga cccgccgaac tgcgcgggcg cgaccgtcgg
     6481 cgtcgaccag gcctacggcg ccatcaagca gagctgcggc tgccatggcg gcggcgcggg
     6541 cggcctgact tttacgaacg cgtcggattt tcgcaacaac atggtcggcg tcaactcgca
     6601 gaccgcggcg atgaagatcg tcaccgccgg cgacatcaat cagagctacc tgatctacaa
     6661 gatcctcaat cagcacagca aggtcgtcgg cgggcggggg aaccaaatgc cgctcggcaa
     6721 gtcgctgagc gacaaccaga agtgcctgct gatcaattgg atcaagagcg gcgcgaagtg
     6781 atcgcgtagc ggacgagcga agaggtcggc agcctcgcgg ccgccgacct cttttcttca
     6841 aagctcggcg ccaggcgccg gacgctcagg acttgccgcc ggtgagcatc tcctgcatgc
     6901 cggggatgtc gccgagcagc ggcatgagga tgacctcgat gcggcggttc ttggccttgt
     6961 cgtcctcggt cttgttgtcg gtgatcgggt cgaactcgcc gaagccggcg gcgccgatgt
     7021 tcttcgaggt catgccctgg ctgatgaggt actgcacgac ctcgacggcg cgcgcggtcg
     7081 agaggtccca gttgttcttg aagcgggccg tcttgatcgg gacgttgtcg gtgtggccgg
     7141 cgaccaggaa ctcgcggtcg ttgagcgact tgagcgcgct ggcgaggttg gcgagcgcgg
     7201 tctgaccctc gggcttgagc tcggtcttgc cggagtcgaa caggatgctg ctcgccagct
     7261 tgacgaccaa gcgacccttg cgaaactcga cctggatcgt gccggcgtcg acgagcgcct
     7321 tgagcttgcc gaacaggtcc ttgtagacct gcagctcggc gttacgcttc tgcttctcgg
     7381 cgcgcagggc cttgagctcc tcggcggtcg cgccggcctt cttggcgagg tcggcgtttt
     7441 gctcctcgag caccttgatc tcgccgctga gccgggtgat gtcggcctcg agcgaagcga
     7501 tcttctgctg cagcgtctgg ttctcctcgg tcagctgctt ggtcttgccg cgctcggcgc
     7561 cgagctcacc gttggtagcc tcgagcttct tgttggcctc cgcgagggcg gcctcgagtt
     7621 ctttgcgttt gcaaccgctg agggccagcg tggagagaag gagagcgacg acgatgctgc
     7681 gcatgcgcca cagggtaacg aaacccggcc ggttttttga tcccaaactg cgggctttgt
     7741 aggatgcgac gaccaagatc tggccatcct cgccaagatc tcggcaagct cttgagatcc
     7801 aaggaaaaat gcccaaagcc cgagagcgtt ctgagtccgc gcgtggcgcg cgaacgcgcg
     7861 acactgtgtc tggtttgccg cgcccgtggg gcgagttcct cggcatcgtg ctgctcgcca
     7921 tcggcgtgct gatggccggc ggcctgtgct cgtaccagtt cggcacgggc tacctgatgg
     7981 gccccgtcgg tcatctggcg gccagcgccc tgtacgcgac gctcggcatg ggcgcgtacc
     8041 tgctggtgct cggcgtgctc ggcctcggcg tcaaggcgct gctcggcgag acgatggaga
     8101 tccgcgcagg tgagggcctc ggcttcgccg gggcgacgat cgccggctgc gtgctgctgc
     8161 acgtcacctt ccccgagtac cgcacccacg gcctgacctc cggtggcctc accggcgagc
     8221 tgctcggcga ggtcggcctc ggcctgttcg accgcgccgg cacctacctg atcgcattcg
     8281 cgctgctgtt cgtcggcctg ttcgcctcga cgccgctgtc ggtcgagcac ctcgtcatcg
     8341 gcgtgcggct cgccgtgcgc ggcacgatcg ccgtcggcgc atacctgtgg ggcggcctcg
     8401 tcggcctcta cgacgcgctg cgcggccgac ccgacgagca cagcgagcag gacgaggaag
     8461 aagagtacga ggaggatgcg gaggaagagg acgagctcga cgaggaggcc gaggaggaag
     8521 aagaagagga ggaggagccc gctccgccgc cgaagccgcg acgcagccgc aaggccgccg
     8581 cgccggccga gcccgtcgcc ttgccggccc cggcagtcgc accggccgcc atgaccgaac
     8641 ccgcggccga gccgcgcaag aatcgcaagc tcccgcgccg cccggaggac atgccggaga
     8701 tcgtcgtcgc cggaccgacg cggccggagg aggccgccaa gcccgagccg aagaagaagc
     8761 cgaaggccga gccggtcgtc gaggaggccg aggacgagtc ggccgacgcc gtcgagccgg
     8821 ccgccaacag cggcgcgccg aagaagcgcc gcaaggcgcc cgcgcccgag atcgtcgagc
     8881 ccgaggacga ggacgacgcg tcggtggccg acgagcgcga ggcgccggcc gaggagcccg
     8941 cgccggtgtc ccgcaccggc aagaaaccgc cgccgcgccc ggccgagatc atgccgcccg
     9001 cggccgtcac accggcgccg gtgcagctgc cgctggccga ggccgccaag ccgcccgagc
     9061 gcaccccggc gatccccggc atcgtgcgtc tcactgcggg gccgttcctg ttgccgccgt
     9121 cgaagctgtt cgagctgccc gagcaggcgg tgaccgaggt cgacaagggc ttcgtgctcg
     9181 agcaggccga gcgcatcgag cacgcgctgt cgaccttcaa ggtcaccggc aaggtcacca
     9241 agatccaccc gggtccggtg atcacgcgct acgagttcaa gcccgacccg ggcatgaagc
     9301 tctcgcgcat cgagagcctc gagaatgacc tggcgatggc gctcgaagcg atctcgatcc
     9361 gcatcctcgc gccgatcccc ggcaaggcga cggtcggctt cgaggtcccg aacgtggtcc
     9421 gcgagacggt ggcgatcaag gagatcctcg acagcgaggg cttcggcccc gagcggacct
     9481 ttaaactgcc gatggcgctc ggcaagaaca tcaccggcaa gccgatcacg atcgacctgg
     9541 cgaaggcgcc gcacctgctg gtcgccggcg cgaccggctc cggcaagtcg gtcggcgtca
     9601 acgcgatgat ctgctcgctg ctgtactcgt gcacgcccga ggacgtgcgc atgatcatga
     9661 tcgacccaaa gatgctcgag ctgagcatct acaaggacat cccgcacctg ctgttgccgg
     9721 tgatcaccga cgccgaggcg gcgaacctgg cgctgcgctg ggccgtcgac gagatggagc
     9781 gccggtactc cgtgctcagc gaggcgcagg tgcgcgacat cggcggctac aacaagaagc
     9841 tcccgatctt gctcgagcag ttcgagtccg agaagaagat gctcgagatg gaggccgacc
     9901 gcgccaacct cgactccgag gaggagggcg aggacgcggt ggcgaccgag ttcctcgggg
     9961 gcgtcgcgtt cgacgcggac ggccatccga tgcgccagct cggcagcgac atgcacggcg
    10021 cgaagctcgg cgaccggccc gagaagatgc cgtacatcgt cgtgatcatc gacgagttcg
    10081 cggacctgat gatggtcgcg agcaaggagg tcgagacctc ggtcgcgcgg atcgccgcca
    10141 aggcgcgcgc gtgcggcatc cacctgatcc tcgccacgca gcggccgagc gtcgatgtca
    10201 tcacgggaac gatcaagaac aacttcccgt cgcgcatcgc gttccaggtg accagcgaca
    10261 tcgactcgcg cacgatcctc gacggcaagg gcgccaagca gctgctcggc atgggcgaca
    10321 tgctgtacat ggaccgcggc gcgcagccgc agcgcgtgca cggctgctac gtctcggagg
    10381 aggagatctt caaggtcgtc gccttcatca agaagcaggc gcggccggtg tacaaccacg
    10441 cgatcgcggc gaagcgcgag gaggacctcg aggaggagcc gagcgaccgc cgcgccgacc
    10501 ccctctacga caaggcggtg cagatcgtcg ccg
//
LOCUS       DASWNJ010000664         1580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_295727, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000664 DASWNJ010000000
VERSION     DASWNJ010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295727"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1469)
                     /locus_tag="VGB85_11115"
     CDS             complement(<1..1469)
                     /locus_tag="VGB85_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0134627.1"
                     /translation="MPPQQAASYLIARLPLVHRPHYPREQPKKSPMEREHDLAAVVTS
                     AQRHLVRFVPARHRSSPGPRTGTFRATRQAASPRLGAGTRFTNVHRRKQRTRRSSAED
                     SSRIHHRVRRRFAATSSLSARQRPRDASRDRARAFSRCSRGASRRSCPAMEVIGKDGR
                     YELRGELGKGTRGVVYAAIDRQRRQPVVIKRFDTTIAAGSLAKYAEIIKRIAQSGVVG
                     VVSPHEVVTAGPAPFAVFPALDGSSLESMLAGGPLPWARAMEIVRDCAETLAAVHAAT
                     GVTHGNLKPGNVWIAEKGKALVLDLGTAGLGPPGPVRRGAELVEYRAPEQLDGAPGDA
                     RADVFALGVLLVEMTTGIHPFAGATAFKAAHKLLQTPPDPATVTRGLSVSGAQEVAKF
                     MARALAGNPDERHADPRAFAAALAYVRKVVGAPAPLRTERAPEASSPPPAAASTPDPT
                     TMLRIPGLSELFKRTPATPPAPATIAKEPAPAEPTALAP"
BASE COUNT          228 a          520 c          575 g          257 t
ORIGIN      
        1 tggggcgcca gcgccgtggg ttcggcgggc gcaggctctt tggcgattgt cgccggtgct
       61 ggcggagtcg ccggcgtgcg cttgaagagc tcgctcaggc ccgggatccg cagcatggtc
      121 gtcgggtccg gggtcgacgc ggccgcagga ggtgggctgg acgcttcggg cgctcgctcc
      181 gtccgcaatg gggccggcgc gccgacgacc ttgcgcacat atgccagcgc cgccgcaaag
      241 gcccggggat ccgcgtggcg ctcgtcgggg tttcccgcga gcgcgcgagc catgaacttg
      301 gcgacctcct gggcgccgct gaccgacagg ccccgcgtga ccgtggcggg gtccgggggc
      361 gtctgcagga gcttgtgtgc ggccttgaac gcggtcgcgc cggcgaacgg gtggatgccg
      421 gtggtcattt cgacgagcaa tacgccgagg gcgaacacgt ccgcgcgcgc gtcaccggga
      481 gcgccgtcga gctgctccgg cgcgcggtat tcgacgagct ccgcaccgcg tcgcaccggt
      541 ccgggcggcc cgagtccggc ggttccgaga tccagcacca gcgctttgcc cttctccgcg
      601 atccacacgt ttccgggctt gaggtttccg tgggtcacgc cggtggccgc atgcacagcc
      661 gccagggtct ccgcgcaatc tctcacgatc tccatcgcgc gcgcccacgg gagggggccg
      721 ccggccagca tcgactcgag cgagctgccg tcgagcgcgg ggaacaccgc gaacggggca
      781 gggcccgccg tcacgacctc atgcgggctc acgaccccga cgaccccaga ctgcgcgatc
      841 ctcttgataa tctccgcata ctttgcgagc gagccggcgg cgatcgtcgt gtcgaaacgc
      901 ttgatgacga ccggctgccg cctctgccga tcgatggcgg catacaccac tccgcgcgta
      961 cccttgccga gctcgccacg gagctcgtag cgcccgtcct tgccgatgac ctccatcgcc
     1021 gggcaggatc tccgcgaagc gccgcgggag caacgtgaaa atgcgcgtgc gcggtcgcgc
     1081 gaagcatcac gtggtctctg tcgagcggac aaagacgatg tggcagcaaa ccggcggcgg
     1141 acgcggtggt ggatgcgcga gctgtcctcc gcgcttgacc gacgagtacg ctgtttccgg
     1201 cgatgcacgt tcgtgaagcg tgtacccgcg ccgaggcgag gcgaggcggc ctgccgggtc
     1261 gcgcgaaatg tgccggtccg tgggccgggc gagcttcgat gtctcgcggg tacgaagcgc
     1321 acgagatggc gctgcgcgct ggtgaccacc gcggcgagat cgtgttctcg ctccatgggg
     1381 ctttttttcg gttgttctct gggatagtgt ggtctgtgaa ccagtggtaa acgagcgatc
     1441 agatatgaag ctgcctgctg ggggggcaag atcggacgat cgtgggggcc gtacgcatgg
     1501 agctacgccc ataccggccg gccgccccga tccgctcata gggaccgtga tcgaggggcg
     1561 ctacatgatc gagcgcctga
//
LOCUS       DASWNJ010000665         1623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_471301, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000665 DASWNJ010000000
VERSION     DASWNJ010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471301"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VGB85_11120"
     CDS             <1..763
                     /locus_tag="VGB85_11120"
                     /inference="COORDINATES: protein motif:HMM:NF026760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta-hydrolase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0134628.1"
                     /translation="PGVWNLVAGAPGHHEAQRVWTVAPASPDTLTLVLSPVPPAPAPE
                     PVAVAVVQPVPPPVPVRPPPAREPRLELGLGLGLAGAVALGIGTGLLIQHRRGYAHFE
                     AAPNNAGFVAALSASTTGAALVGVGTGLAAAAATAGLAPARRLDRWLWSEVAAGGAVT
                     LIGTAWYASEWQRVQRDLYDSRSAAAHDAGAQRRETAAAAVLGAGAGLMVGAGAALLT
                     RTLIRLHGRRGAARHALGGGPGPLGGPGFQIEGRF"
     gene            836..>1623
                     /locus_tag="VGB85_11125"
     CDS             836..>1623
                     /locus_tag="VGB85_11125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-like YCDxxxxGGGW domain-containing
                     protein"
                     /protein_id="HEY0134629.1"
                     /translation="MARISLRPIRLAGLIATTAALAATAATAAVGGCHVSGCREGDQA
                     CTMLAETTSTADTSGGQTTGAPTSSIDTSSSGASEVTLTEADTSTTPGVCGDAVLDDG
                     EACDDGNAALDDGCLPTCELARCGDGHTFIGVEQCDDGDEDDTDECTSLCKLPRCGDG
                     FVHPPELCDAGEANSDKVYGGCGSNCQPGPGCGDGKLNGPEDCDDENTDPNDGCLVGC
                     IEATSCKQILEVTPGAPSGTYRIWPTALGGGIDLAAYCDMESDGG"
BASE COUNT          211 a          601 c          598 g          213 t
ORIGIN      
        1 cccgggggtg tggaacctcg tcgcgggtgc gccgggccac catgaagcgc agcgcgtgtg
       61 gacggtggcg cccgcgagcc ccgacacgct cacgctcgtg ctctcgcccg tgccacccgc
      121 gcctgcgccc gagccggtcg ccgtcgccgt cgtgcagccg gtgccgccgc cggtgccggt
      181 gcgcccgcca cccgcccgtg aaccgcggct ggagctcggg ctcgggctcg ggctcgcggg
      241 ggcggtcgcg ctcggcatcg gcaccggcct gcttatccag catcgtcgcg gctacgcgca
      301 cttcgaggcg gccccgaaca acgccggctt cgtcgccgcg ctgtccgcga gcacgaccgg
      361 ggcggcgctg gtcggcgtcg gcaccgggct ggccgcggcc gcggccacgg ccggcctcgc
      421 acccgcgcgg cgcctcgacc ggtggctgtg gagcgaggtc gcggccgggg gcgcagtgac
      481 gctcatcggg accgcgtggt acgcgagcga gtggcagcgc gtgcagcggg acctgtacga
      541 cagccgctcc gctgcggcgc acgacgcggg cgcgcagcgg cgcgagaccg cggcggcggc
      601 ggtgctcggg gccggcgcgg ggctgatggt cggggccggc gcggccctgt tgacgcggac
      661 cctgatccgc ctgcacggtc gtcgcggggc cgctcgtcat gccctgggcg gcggacccgg
      721 cccgctcggt ggccccggct tccagatcga gggccgcttc tgatccaccc cactttgctt
      781 ccctcgccgc gtttttaatg tagcttcacc ttttggcttg tcggtccgca tccccatggc
      841 gcgcatctcc ctcagaccca tccgcctcgc cggcctgatc gccacgaccg ccgcgctcgc
      901 cgcgaccgcc gcgaccgccg cggtcggggg ctgtcacgtg tccggttgtc gtgaaggcga
      961 ccaggcctgc acgatgctcg ccgagaccac ctcgaccgcg gacacgagcg gcgggcagac
     1021 gaccggcgcg ccgacctcga gcatcgacac cagctcgtcg ggcgcctcgg aggtcacgct
     1081 gacggaggcg gacacgagca cgacgcccgg ggtctgcggc gacgccgtgc tcgacgacgg
     1141 tgaggcgtgc gacgacggca acgccgcgct cgacgacgga tgcctgccga cctgcgagct
     1201 cgcgcgctgc ggcgacggtc atacgttcat cggcgtcgaa cagtgcgacg acggcgacga
     1261 ggacgacacc gacgagtgca ccagcctgtg caaattgccc cgctgcggcg acggcttcgt
     1321 ccacccgcca gagttgtgcg atgcaggcga agccaacagc gacaaggtct atggcgggtg
     1381 cggtagcaac tgccagccgg gacctggctg cggcgatggt aagctcaacg gtcccgagga
     1441 ctgtgacgac gagaacaccg acccgaacga cggctgcctg gtcggctgca tcgaggcgac
     1501 cagctgcaag cagatcctcg aggtgacgcc cggggccccg tccggcacct atcgcatctg
     1561 gccgacggcg ctcggcggcg gcatcgacct cgcggcctat tgcgacatgg agagcgacgg
     1621 cgg
//
LOCUS       DASWNJ010000666         1664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_82182, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000666 DASWNJ010000000
VERSION     DASWNJ010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82182"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            890..>1664
                     /locus_tag="VGB85_11130"
     CDS             890..>1664
                     /locus_tag="VGB85_11130"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0134630.1"
                     /translation="MELLSAIVAWSLRNRALVLVATLIFVALGIRSATQLPMDAVPDI
                     TSVQVQIATAAPALAPVEVEQYISIPVERAMSGLPRVLEIRSLSKYGISLVTVVFEDG
                     TDIYRARQLVGERMSQARSAISPRYGTPELGPISTGLGEIYQFTLRAEDPEKNDLMAL
                     EELLAWYIGPQLRTVPGVVDVNSFGGFEREYQVVLRPERLQALGLAIGDVVHALEQSN
                     ANAGGGYIEHNQEHFVIGTDGLVKSREDLERVVLGATPQG"
BASE COUNT          259 a          617 c          565 g          223 t
ORIGIN      
        1 gacgcgggtc ctcccagagc acgtgctcgc cgccccatct cgcgcgctta tcataacact
       61 ctcaccgacc ctgggccgcg tgaccggagg gacgaggggg cgcctgcgcg cgagccctgg
      121 gtcctcccgc tgcttccccc gccagccggc ccctaccccc cgggcgaaca ccatcggcgc
      181 cccgcttgtc gcgagcgagc aagatgtcgc gcgaccgcct tcatcctcgc ggaggcctga
      241 accgcgtcgc ggcgcggatc ggcgagaggg cgcgtatctc gatctcgtcg ggctgcgtga
      301 cgcggccgtc ggggtccgcg acgatatcag cacacatcga catgaacgcc gaatgcgggc
      361 actttctctc gcgccccgag agctcgcgta cctcgatatc gtcggttcgc gcgacgagga
      421 cgttcggccc cgcgacgaaa ttgttccggc gacaagcggg gcgcagacga tgccgttccg
      481 gggtagaggg gccggcggcg ggggaagcag cgggaggacc cagggctcgc gcgcagacgc
      541 cccaccgcca gtcgagagcg gcgagagagc agcggtgaaa gaagcatgtc cttccggaca
      601 ggtggggcgg cgagcacgtg ccctgggagg acccgcgtcg cagggccccg cagtccgtcg
      661 gcccctcccc ggcccatgta gcctcgcccc gcgtaccgcg atcacgccct gctccggcgc
      721 actacacggc ccgagcacgc cccgccccgg cgcatcacac caaacgagca cgccccaccc
      781 cgtcgcatca caccaaacga gcacgccccg ccccgccgca tcgcaccaaa cgagcacgcc
      841 ccgccccgcc gcagagcggg cgcgcccaca aacagggggg cggtgagccg tggagctgct
      901 ctcggcgatc gtcgcgtggt ccctgcgcaa tcgggccctg gtgctggttg cgaccttgat
      961 cttcgtcgcg ctgggcatcc gttcggcgac ccagctgccg atggacgcgg tcccggacat
     1021 cacgagcgtg caggtgcaga tcgccacggc agcgcccgcg ctggcgccgg tcgaggtcga
     1081 gcagtacatc agcattccgg tcgagcgcgc gatgtccggc ctgccgcgcg tgctcgagat
     1141 ccggtcgctc tcgaagtatg gcatctcgct ggtgacggtc gtcttcgagg acggcaccga
     1201 tatctaccgg gcgcgccagc tcgtcggcga gcgcatgtcg caggcgcgct cggcgatctc
     1261 gccgcgctac ggcacgccgg agctcggccc gatctcgacc gggctcggcg aaatttatca
     1321 gttcacgctg cgcgccgagg accccgagaa aaacgacctg atggcgctcg aggagctgct
     1381 cgcctggtac atcgggcccc agctgcgcac ggtccccggc gtggtcgacg tcaacagctt
     1441 cggcggcttc gagcgcgagt accaggtcgt gctccggccc gagcggctgc aggcgctcgg
     1501 cctggcgatc ggcgacgtcg tgcacgcgct cgagcagtcg aacgccaacg ccggcggcgg
     1561 ctacatcgaa cacaaccagg agcacttcgt catcggcacc gatggcctgg tcaagagccg
     1621 cgaggacctc gagcgcgtgg tcctcggcgc gacgccgcag ggcg
//
LOCUS       DASWNJ010000667         1998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_577687, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000667 DASWNJ010000000
VERSION     DASWNJ010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577687"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VGB85_11135"
     CDS             <1..564
                     /locus_tag="VGB85_11135"
                     /inference="COORDINATES: protein motif:HMM:NF014907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEY0134631.1"
                     /translation="LPATVGNHLAGKLGKSVPGTDHPAFAEAENGVRDRMRHYLSING
                     KHSVDEFHRELGLVMWDHCGMARNEAGLKKALQVIPEIRERFWKQVRVPGVGAEFNQN
                     LERAGRVADFLEFGELLCLDALTRDESCGGHFREEHQMDDGEAKRNDERFAHAAVWEY
                     KGADAAPARHEEPLEFEHVHLATRVYT"
     gene            578..1423
                     /locus_tag="VGB85_11140"
     CDS             578..1423
                     /locus_tag="VGB85_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEY0134632.1"
                     /translation="MSSTSAPNIKLTLRIWRQKDTKTPGKLETYKLDAVSPDMSFLEM
                     LDVLNDTLTREGGDPIAFDHDCREGICGSCAMMINGRAHGPRKGTTVCQLHMRSFKDG
                     DTITVEPWRAQPFPVIKDLSVSRASLDRIIAAGGYVSINTGSTVDGNAIPIGKKDADE
                     AMDYASCIGCGACVAACPNAAAMLFTGSKVAHLAKLPQGQVERAGRVRSMVAAMDAEG
                     FGSCTNHGECSEACPKEIPFDAIVMLNREYMRATVLGKESLPARSEPATSVGDRGRCS
                     SVPSS"
     gene            complement(1464..>1998)
                     /locus_tag="VGB85_11145"
     CDS             complement(1464..>1998)
                     /locus_tag="VGB85_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134633.1"
                     /translation="TCGNTTQAGGGGGGGGAGGCGGTEGLSGSAGGGSFGLVVISSSI
                     QVASSIILPAKAGNGGKGGTGGNGGVGKAGGAGGKGINGGDQSDGGGDGAAGAAGSDG
                     GGAGGGAGGCGGISVAIVRVNGSFVGKTNTDIQMGVAGDPGVGGDGGFEGNGGNKPQA
                     PTGAQGCKGVAVDEIDL"
BASE COUNT          319 a          767 c          642 g          270 t
ORIGIN      
        1 ctcccggcga cggtcggcaa ccacctcgcc ggcaagctcg gcaagtcggt gcctgggacc
       61 gaccacccgg ccttcgccga ggcggagaac ggggtgcgcg accggatgcg ccactacctg
      121 tccatcaacg gcaagcacag cgtcgacgag ttccaccgcg agctcggcct cgtcatgtgg
      181 gaccactgcg gcatggcccg caacgaggcc ggcctcaaga aggccctgca ggtcatcccc
      241 gagatccgcg agcgcttctg gaagcaggtc cgcgtgcccg gcgtcggcgc cgagttcaac
      301 cagaacctcg agagggccgg ccgtgtcgcc gacttcctcg agttcggcga gctgctgtgc
      361 ctcgacgcgc tgacccgcga cgagtcctgc ggcggccact tccgcgagga gcaccagatg
      421 gacgacggcg aggccaagcg caacgacgag cgcttcgccc acgcggcggt gtgggagtac
      481 aagggcgccg acgcggcccc cgctcgccac gaggagccgc tggagttcga acacgtccac
      541 ctcgccaccc gcgtgtacac ctgaggagac cacgaccatg tcgtccacgt ccgctcccaa
      601 tatcaagctg acccttcgca tctggcgcca gaaggacacc aagaccccgg gcaagctgga
      661 gacctacaag ctcgacgcgg tctcgccgga catgtcgttc ctcgagatgc tcgacgtcct
      721 caacgacacg ttgaccaggg aaggcggcga cccgatcgcc ttcgaccacg actgccgcga
      781 gggcatctgc ggctcgtgtg cgatgatgat caatggccgc gcccacggcc cgcgcaaggg
      841 caccacggtg tgccagttgc acatgcgcag cttcaaggac ggcgacacga tcacggtcga
      901 gccgtggcgc gcccagccct tcccggtgat caaggacctc agcgtcagcc gggccagcct
      961 cgaccgcatc atcgccgccg gcggctacgt gtcgatcaac accggctcga ccgtcgacgg
     1021 caacgcgatc ccgatcggca agaaggacgc cgacgaggcg atggactacg cctcgtgcat
     1081 cggctgcggg gcctgcgtcg ccgcctgccc gaacgccgcc gcgatgctgt tcaccggctc
     1141 caaggtggcc cacctcgcca agctcccgca gggccaggtc gagcgcgccg gtcgtgtgcg
     1201 cagcatggtc gcagccatgg acgccgaggg cttcggcagc tgcaccaacc acggcgagtg
     1261 ctccgaggcg tgcccgaagg agatcccctt cgacgcgatc gtgatgctca accgcgagta
     1321 catgcgcgcg accgtgctcg gcaaggaatc gctccccgca cgcagcgagc ccgcgacctc
     1381 cgtcggagat cgcgggcgtt gttcgtcggt gccctcgagc tgagcacctg gccccgcgga
     1441 caggtccgcg gggccgtcac cgcttacagg tcgatctcgt cgaccgcgac gcccttgcag
     1501 ccctgcgcgc ccgtgggagc ctggggcttg ttgccgccgt tgccctcgaa gccgccgtct
     1561 ccgccgacgc cgggatcacc cgcgacgccc atctggatgt cggtgttggt cttgccgacg
     1621 aacgacccgt tgacgcggac gatcgccacc gagatgccgc cgcaaccacc tgcaccacca
     1681 cccgcgccgc cgccgtcgct gcccgcggcc ccggccgcac cgtcgccacc gccgtcgctc
     1741 tggtcgccgc cgttgatgcc cttgccaccg gccccgccgg ccttgccgac cccgccgttt
     1801 ccgccggtcc cgcccttgcc gccgttgccg gccttcgcgg gcaggatgat cgagctcgca
     1861 acctggatcg acgaggagat cacgacgagg ccgaaggaac caccgccggc gctgccggac
     1921 aggccctcgg tgccgccgca gccgcccgcg ccaccaccac caccgccgcc gccggcctgg
     1981 gtcgtgttac cgcaggtg
//
LOCUS       DASWNJ010000668         2215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_647868, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000668 DASWNJ010000000
VERSION     DASWNJ010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647868"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="VGB85_11150"
     CDS             complement(<1..856)
                     /locus_tag="VGB85_11150"
                     /inference="COORDINATES: protein motif:HMM:NF014416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134634.1"
                     /translation="MKFFEIIPPGTKFPFIRAAKYFILVSVVLLLGSIAAMVYNVSAH
                     GSALNFGIDFAGGSSIRVALAAEKNVQIQDVREALEAAGYEGVSAVTVPDAEGQVLIH
                     VKDVASIDKAQGDACKAAVEAYPGAKLKRFSYADGASKMFMLFETQPTYSELETLLAQ
                     AGCEGKIDKGTGKMGEFPVELSLIGIGAKIQDDLNKAFGAGAVTDIVSSETVGAKVGS
                     QLKNDGIKSLLYAIGFIFLYVMLRFDLRFAPGGIIALAHDAAITIGAFALTWKEFNLQ
                     TIAALLTII"
     gene            complement(877..>2215)
                     /gene="secD"
                     /locus_tag="VGB85_11155"
     CDS             complement(877..>2215)
                     /gene="secD"
                     /locus_tag="VGB85_11155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY0134635.1"
                     /translation="LELEVVADDAGAGSTPDVVNLRISEAARDKVLEESSDFRRLLKI
                     IERAAVLEMRMVDDETPFGDTGKPYLRALYDAGLVTEGMGIAVNDMGDYGNEKGANVK
                     ESYAFLARDKATLEQFFDNLPPQWRLPATHMIAYGVQPIVTRRGAEPEAIWRTYVVKS
                     RAEVTGESIVTANVGFDQQTGTPEVNLKLDRVGADRFEAMSGENIGRKMAIMLDDEVM
                     SDPVFNDRIPGGNVRITMGQTPGQSVREAANDLVKVLKSGSLPARMVKEFEIRVGADL
                     GKEAVESGFWAFVFGLSAVVLFMGFYYRIAGWISVFALVLNVLLMLAAMAFFQATLTL
                     PGIAGIVLTLGMAVDANVIIYERIRELLRDGYTARAAIEAGYDRAFTTILDSQLTTAI
                     AGIVLWQYGSGPIRGFAITLLIGITTSVFTAVFCTRLFFDFQANRRGFDRVSI"
BASE COUNT          360 a          702 c          788 g          365 t
ORIGIN      
        1 cgatgatcgt cagcagcgcg gcgatcgtct ggaggttgaa ctccttccag gtcagcgcga
       61 aggcgccgat cgtgatcgcg gcgtcgtgcg cgagggcgat gatgccgccc ggcgcgaagc
      121 gcaggtcgaa gcgcagcatc acgtagagga agatgaagcc gatcgcgtag agcagcgact
      181 tgatgccgtc gttcttgagc tggctgccga ccttggcgcc gacggtctcc gacgagacga
      241 tgtcggtcac ggcgccggcg ccgaaggcct tgttgaggtc gtcctggatc ttcgcgccga
      301 tgccgatcag gctcagctcg accgggaact cgcccatctt gccggtaccc ttgtcgatct
      361 tgccctcgca gccggcctgg gcgagcagcg tctcgagctc gctgtaggtc ggctgggtct
      421 cgaacagcat gaacatcttg ctcgcgccgt cggcgtacga gaagcgcttg agcttggcgc
      481 cggggtaggc ctcgaccgcg gccttgcagg cgtcgccctg ggccttgtcg atgctggcga
      541 cgtccttgac gtggatcagc acctggccct cggcgtccgg cacggtgacg gcggagacgc
      601 cctcgtagcc ggcggcctcg agcgcctcgc ggacgtcctg gatctggacg ttcttctcgg
      661 ccgcgagagc gacacggatc gacgatccgc ccgcgaagtc gatgccgaag ttgagcgccg
      721 agccgtgcgc ggagacgttg tagaccatcg cagcgatcga gccgagcaac agcacgaccg
      781 agacgaggat gaaatacttc gccgcgcgga tgaacgggaa cttggtcccg gggggaatga
      841 tctcgaagaa cttcatggga tgcggtcccg ggggtgtcag atcgacacgc ggtcgaagcc
      901 gcggcggttg gcctggaagt cgaagaacag gcgggtgcag aacaccgccg tgaacaccga
      961 ggtggtgatg ccgatcagca gcgtgatcgc gaacccgcgg atcgggcccg aaccgtactg
     1021 ccagagcacg atgccggcga tcgcggtcgt cagctgcgag tcgaggatcg tcgtgaaggc
     1081 gcggtcgtag ccggcctcga tcgccgcgcg cgcggtgtaa ccgtcgcgca gcagctcgcg
     1141 gatgcgctcg tagatgatca cgttggcgtc gaccgccatg ccgagcgtca gcacgatgcc
     1201 ggcgatgccg ggcagcgtca gggtcgcctg gaagaaggcc atcgccgcga gcatcagcag
     1261 cacgttgagg accagcgcga acaccgagat ccagccggcg atgcggtagt agaagcccat
     1321 gaacaggacg accgcgctca ggccgaacac gaaagcccaa aagccgctct cgacggcctc
     1381 cttgccgagg tcggcgccga cgcggatctc gaactccttg accatgcggg ccgggaggct
     1441 gccgctcttg agcaccttga cgaggtcgtt ggcggcctcg cggacgctct gacccggggt
     1501 ctgacccatc gtgatgcgga cgttgccgcc ggggatgcgg tcgttgaaga cggggtcgga
     1561 catgacctcg tcgtcgagca tgatcgccat cttgcgaccg atgttctcgc ccgacatcgc
     1621 ctcgaagcgg tcggcgccga cgcggtcgag cttgaggttg acctcgggcg tgccggtctg
     1681 ctggtcgaag ccgacgttgg cggtcacgat gctctcgccg gtgacctccg cgcgcgactt
     1741 gacgacgtag gtgcgccaga tcgcctccgg ctcggcgccg cggcgggtca cgatcggctg
     1801 cacgccgtac gcgatcatgt gcgtggccgg caggcgccac tgcggcggga ggttgtcgaa
     1861 gaactgctcg agggtcgcct tgtcgcgcgc gaggaaggcg tacgactcct tgacgttggc
     1921 gcccttctcg ttgccgtagt cgcccatgtc gttgacggcg atgcccatgc cctcggtgac
     1981 gaggccggcg tcgtacaggg cccgcaggta gggcttgccg gtgtcgccga acggggtctc
     2041 gtcgtcgacc atgcgcatct cgagcacggc ggcgcgctcg atgatcttga gcaggcgacg
     2101 aaagtcgctc gactcctcga gcaccttgtc gcgcgcggcc tccgagatgc gaaggttgac
     2161 gacgtccggc gtgctgccgg cgcccgcgtc gtcggcgacg acctcgagct cgagg
//
LOCUS       DASWNJ010000669         2779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_224497, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000669 DASWNJ010000000
VERSION     DASWNJ010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224497"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..543
                     /locus_tag="VGB85_11160"
     CDS             67..543
                     /locus_tag="VGB85_11160"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY0134636.1"
                     /translation="MHSTIGLKINGAVVRLEVAPWVTLLDLLRERLHLTGTKKGCDHG
                     QCGACTVLVDGERINSCLTLAVMRDGAEVTTIEGLADGEALHPVQQAFIDQDAYQCGY
                     CTPGQICSVVGLIREGKAHTESEIRELMSGNLCRCGAYVNIVAAVQQAMRAGGGQP"
     gene            540..1535
                     /locus_tag="VGB85_11165"
     CDS             540..1535
                     /locus_tag="VGB85_11165"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEY0134637.1"
                     /translation="MNNFGYTRASDVAGAVREHAGDDAASFIAGGTNLIDLMKERITT
                     PGRLIDINRLPLREVSGLPDGGLRIGALVSNSALAYHPEVEARYPVLSRAILSGASAQ
                     LRNMATTGGNLLQRTRCYYFYDAALPCNKRRPGSGCPAREGVNRIHAILGTSEHCIAT
                     HPSDMCVALAVLAAKVQVTGPAGDRTIDLADFHRLPGDTPEVDTNLQSGELITAVDLP
                     AEGFAENYTYLKIRDRHSYAFALVSVAVGLKMQGEKIAQARLALGGVAHRPWRDVAVE
                     AMLRGEVPSEALLTEVGRAIVQDARGFGHNDFKIELARRAVVRALRQAIGTEART"
     gene            1532..>2779
                     /locus_tag="VGB85_11170"
     CDS             1532..>2779
                     /locus_tag="VGB85_11170"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY0134638.1"
                     /translation="MSTRHIGRPTSRVDGRAKVTGTAKYVAEFHVPELVYGVVVSGSI
                     ARGTITKIDSSAALAVRGVIEVLTHENAPRLARRDRDHRDMVAPPGSPFRPLQGPEIV
                     YSGQPVALVLAESFELARHAAGLVRVEYAEVAHETRLEAVRGEAYGPPKNKAGYKPPP
                     RPRGNADKAFAAAAVKQDATYTHAMTHHNPMETHATTVVVEHDGTLTIHDKTQGAQNS
                     RDYVAKVFDLPPEQVRVIAPFIGGAFGSGLRPQYQLPLAVMAAKALRRSVRVVLTRQQ
                     MFTFGYRPRVEQRLALGSSQEGRLEAITHDTLSNTSRFEHYCENVVPWSGVMYQCDNV
                     RLDYKIAQLDLYTPIDMRGPGAVSGVYALECAIDELADKLGEDPLALRLRNYAEHDQN
                     KDQPFSSKALRECYQKGAEAFGWA"
BASE COUNT          456 a          869 c         1068 g          386 t
ORIGIN      
        1 cgggcggcgc gcggacgacc ccggggtcgg cggcgaggtc tctcgatgcg ggcatcttcg
       61 gtgcgcatgc acagcacgat cgggctgaag atcaacggcg ccgtggtacg gctcgaggtg
      121 gcgccgtggg tgacgctcct cgatctgctg cgcgagcggc tgcacctgac cggcaccaag
      181 aagggctgtg accacgggca gtgcggcgcc tgcacggtgc tggtcgacgg cgagcggatc
      241 aacagctgcc tgacgctggc ggtgatgcgc gacggcgccg aggtgacgac gatcgagggg
      301 ctcgccgacg gcgaggcgtt gcacccggtg cagcaggcgt tcatcgacca ggacgcgtat
      361 cagtgcggct attgcacgcc gggtcagatc tgctcggtgg tcgggctgat ccgcgagggc
      421 aaggcgcata cggagagcga gatccgtgag ctgatgagcg gcaacctgtg ccgctgcggc
      481 gcgtacgtca acatcgtcgc ggcggtgcag caggcgatgc gcgccggcgg agggcagccg
      541 tgaacaactt cggatatacg cgggccagcg acgtcgccgg ggcggtgcgc gagcacgcgg
      601 gcgacgacgc ggcgagcttc atcgccggcg gcaccaacct gatcgacctg atgaaggagc
      661 ggatcacgac gccggggcgg ctgatcgaca tcaatcgcct gccgctgcgc gaggtcagcg
      721 gcttgccgga cggcgggctg aggatcggcg cgctggtcag caattcggcg ctggcgtatc
      781 accccgaggt cgaggcgcgg tatcccgtgc tgtcgcgggc gatcctctcc ggggcctcgg
      841 cgcagctgcg caacatggcg accaccggcg gtaatctgct gcagcggacc cgctgctatt
      901 atttttatga cgcggcgctg ccgtgcaaca agcgcaggcc cggcagcggg tgtccggcgc
      961 gcgagggcgt caaccgcatt catgcgatcc tcgggacgag cgagcattgc atcgccaccc
     1021 acccgtcgga catgtgcgtc gcgctcgcgg tgctggcggc gaaggtgcaa gtgacggggc
     1081 cggcgggcga tcgcacgatc gacctcgcgg actttcatcg cttgccgggg gacacgcccg
     1141 aggtcgacac gaatctccag agcggggagc tgatcaccgc ggtcgatctg ccggcggagg
     1201 gcttcgccga gaattatacg tacctcaaga tccgcgaccg gcactcgtat gcgttcgcgc
     1261 tggtgtcggt ggcggtcggt ttaaaaatgc agggggagaa gatcgcgcag gcccggctcg
     1321 cgctcggcgg ggtcgcgcac aggccgtggc gggatgtcgc cgtggaggcg atgctgcgcg
     1381 gcgaggtgcc gagcgaggcg ctgttgaccg aggtcggtcg ggcgatcgtg caggacgcgc
     1441 ggggctttgg ccacaatgac ttcaagatcg agctggcacg ccgcgcggtg gtccgcgcgc
     1501 tgcgtcaggc gatcgggacg gaggcccgga catgagcacg aggcacatcg gaaggccgac
     1561 cagccgcgtc gacgggcgcg cgaaggtgac ggggacggcg aagtacgtcg cggagtttca
     1621 cgtcccggag ctcgtgtacg gggtcgtggt gtcgggctcg atcgcccgcg gaacaatcac
     1681 gaagatcgac agcagcgcgg cgctggcggt ccgcggcgtg atcgaggtgc tgacccacga
     1741 gaacgcgccg aggctcgcgc gacgcgaccg cgaccaccgc gacatggtcg cgcccccggg
     1801 ctcgccgttc cggccgctgc aggggccgga gatcgtgtac agcgggcagc cggtcgcgct
     1861 cgtgctcgcg gagagcttcg agctggcgcg ccacgccgcg gggctggtgc gggtcgagta
     1921 cgccgaggtg gcgcacgaga cgaggctcga ggcggtgcgc ggcgaggcgt acgggccgcc
     1981 gaagaacaag gccggctaca agccgccgcc gaggccgcgc ggcaacgcgg acaaggcgtt
     2041 cgcggcggcg gcggtcaagc aggacgcgac ctacacccac gcgatgacgc accacaaccc
     2101 gatggagacg cacgcgacga cggtggtggt cgagcacgac ggcacgctga cgatccacga
     2161 caagacgcag ggggcgcaga acagccgcga ttatgtcgcc aaggtgttcg acctgccgcc
     2221 ggagcaggtc cgggtgatcg cgccgttcat cggcggggcg ttcggctcgg ggctgcggcc
     2281 gcagtatcag ctgccgctcg cggtgatggc cgcgaaggcg ctgcggcggt cggtgcgggt
     2341 ggtgctgacg cgccagcaaa tgttcacctt cggctatcgc ccgcgggtgg aacaaaggct
     2401 cgcgctcggg tcgagccagg aaggtcggct cgaggcgatc acgcacgaca cgctgtcgaa
     2461 cacctcgcgc ttcgagcact actgcgagaa cgtggtcccg tggtcgggcg tgatgtacca
     2521 gtgcgacaat gttcggctcg actacaagat cgcccagctc gacctgtaca cgccgatcga
     2581 catgcgcggg ccgggggcgg tgtcgggggt gtacgcgctc gagtgtgcga tcgacgagct
     2641 ggccgacaag ctcggcgagg acccgctggc gctgcgcctg cgcaactacg ccgagcacga
     2701 ccagaacaag gaccagccgt tttccagcaa ggcgctgcgc gagtgctatc aaaagggcgc
     2761 cgaggcgttc ggctgggcg
//
LOCUS       DASWNJ010000670         2615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_647958, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000670 DASWNJ010000000
VERSION     DASWNJ010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647958"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VGB85_11175"
     CDS             complement(<1..383)
                     /locus_tag="VGB85_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134639.1"
                     /translation="MPSTPRRHALLAALLAAGCYTSNDGSPDAASTGAASSSSSTGEG
                     TTDASTPTSEQTGTSVTGQVTGDDSSGAVSGTTGEATTGPGPTTGLDTTATTTATTTG
                     EPPAEPAIHYVGRYDDSDPTHVRMG"
     gene            complement(435..917)
                     /locus_tag="VGB85_11180"
     CDS             complement(435..917)
                     /locus_tag="VGB85_11180"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY0134640.1"
                     /translation="MPLTLTCALVTALFSGCTKRGSKDPADPEEAPGPAEQKIAGATL
                     FEKHCAKCHGALGKGKDDVPAVVGEGALSRFNTAQEVFDFVSKEMPDDKPGSLKEGEY
                     WAILAFDLKANGIDVEEALGPQNASSFVLHSEGDKADDKQDAAKGDNTDDKVSSDVNE
                     "
     gene            1185..1757
                     /locus_tag="VGB85_11185"
     CDS             1185..1757
                     /locus_tag="VGB85_11185"
                     /inference="COORDINATES: protein motif:HMM:NF019494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HEY0134641.1"
                     /translation="MEVKKYGNRRLYDTEGSRYVTLEEVAERVRLGEDLRVVDAKTGR
                     DLTQVTLAQLILESRGAARLLPVPLLKQLLRMQDDALAEFFNRYVTWALEVYLRIKQG
                     TRIWNPLAGLGFAAPDLLARLLRQDPAGDDLPPPPFPGEPDVADESDEASDADELAQL
                     RRELDELKRVVHEGREPPPAEPPPRARRSK"
     gene            1870..>2615
                     /locus_tag="VGB85_11190"
     CDS             1870..>2615
                     /locus_tag="VGB85_11190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEY0134642.1"
                     /translation="MRTALLLNPSAGTGTTATQNDLGERLAAALGVPVEAYPTETGEA
                     MLAAAEAVGRAGFERVLVAGGDGSVNLCINGLARAGALARTVVGIIPAGTGNDLATFL
                     GMPTDLDAAIAALAEARPTPLDLGEMNGLYFANASCGGYFGEVSEATSSELKSVARRL
                     AYVLAGATVFLQHTPTSTRLTADTEFGPLEWAGPLSMFAVCNGMTVGGGRPLAPEASV
                     SDGLFDVFFVKEVSGTGLASVLLQMGRGLHL"
BASE COUNT          313 a          961 c          936 g          405 t
ORIGIN      
        1 cagcccatgc gcacgtgcgt cgggtcgctg tcgtcgtatc ggccgacata gtggatcgcg
       61 ggctcggccg gcggctcgcc ggtcgtcgtt gccgtggtcg tggcggtcgt gtcgaggccg
      121 gtcgtcggcc ccgggccggt cgtcgcctcg ccggtcgtgc cactgaccgc gccgctgctg
      181 tcgtcgccgg tgacctggcc ggtcacgctc gtcccggttt gttcgctggt cggcgtgctc
      241 gcgtcggtcg ttccctcgcc ggtcgagctc gacgaactcg cggcgccggt gctcgccgcg
      301 tcgggtgagc cgtcgttgct ggtgtagcag ccggcggcga gcagggcggc gagcagggcg
      361 tgacggcgcg gcgtcgaggg catcggcgca ctatatcagc cggccctggc cggccaagac
      421 cgcgttatcg cccgctactc gttgacgtcc gacgagacct tgtcgtcggt gttgtcgccc
      481 ttggccgcgt cctgcttgtc gtcggccttg tcgccctccg agtgcagcac gaagctcgac
      541 gcgttctgcg ggccgagggc ctcctcgacg tcgatgccgt tggccttgag gtcgaacgcg
      601 aggatcgccc agtactcgcc ctccttgagg ctgccgggct tgtcgtcggg catctccttg
      661 ctgacgaagt cgaacacctc ctgcgcggtg ttgaagcggg acagcgcgcc ctcgccgacg
      721 accgccggga cgtcgtcctt gcccttgccg agcgcgccgt ggcacttcgc gcagtgcttc
      781 tcaaacaacg tcgcgccggc gatcttctgc tcggcggggc cgggcgcctc ctcggggtcg
      841 gccgggtcct tcgagccacg cttggtgcag ccggagaaca gcgccgtgac gagcgcgcag
      901 gtcagcgtga ggggcatgat ggagttggtt cgggtcgagc gcatggggtg cctccgtcgt
      961 ggtgcgtgac tgtcttgggg caagtcggcg cgcggggcaa agcgccggta cggggcctgc
     1021 gtggggcggc gcaggcgtcc gcgtggcccc cgtggatgcc gccgcgggcc ggcgccgggt
     1081 catgcgcgag cgcttcgcgc agtcagcgca actcgcctca cggggtctct tcgcacctgc
     1141 acaacgccgc cgctgcgccg gtgctttcgt gataggtcag cgtcgtggag gtcaagaagt
     1201 acggcaaccg tcggctctac gacaccgagg gcagccgcta cgtgacgctc gaggaggtcg
     1261 ccgagcgcgt gcgcctcggc gaggacctgc gggtcgtcga cgccaagacc ggccgcgacc
     1321 tcacccaggt gaccctcgcc cagctgatcc tcgagagccg cggggccgcg cgcttgctgc
     1381 cggtgccgct gctcaagcag ctgctgcgca tgcaggatga cgcgctcgcc gagttcttca
     1441 atcgctacgt cacctgggcg ctcgaggtgt acctgcggat caagcagggc acgcgcatat
     1501 ggaacccgct cgcgggcctc ggcttcgccg cgccggacct gctcgcgcgt ctgctgcgtc
     1561 aggacccggc cggcgacgac ctgccaccgc cgcccttccc cggcgagccc gacgtcgccg
     1621 acgagtccga cgaggcgagc gacgccgacg agctcgcgca gctgcggcgc gagctcgacg
     1681 agctcaagcg cgtggtgcac gagggccgcg agccaccgcc ggccgagccg cccccgcggg
     1741 cccggcgcag caagtagcgg acggcggacc ccggcttcgt cgatcgccga cgtccggcgt
     1801 tcccggcgat ccaccgcgcg tccgcgatgc gacgcgagcc acggcgatcg accggatgca
     1861 agctggtgcg tgcgcactgc cctgctcctc aatcccagcg ccggcaccgg cacgaccgcg
     1921 acccagaacg acctcggcga gcgcctcgcg gcggccctcg gcgtgcccgt ggaggcttac
     1981 ccgactgaaa ccggcgaggc gatgctcgcg gccgccgagg cggtcgggcg cgcgggcttc
     2041 gagcgggtgc tcgtcgccgg cggcgatggt tcggtcaacc tgtgcatcaa cggcctggcc
     2101 cgcgccgggg cgctcgcgcg gaccgtcgtc ggcatcatcc cggccggcac cggcaacgac
     2161 ctcgccacct tcctcggcat gccgaccgac ctcgacgcgg cgatcgccgc gctcgcggag
     2221 gcgcgcccga ccccgctcga cctcggcgaa atgaacggcc tgtacttcgc caacgcctcg
     2281 tgcggcggct acttcggcga ggtctccgag gcgaccagca gcgagctcaa gtcggtcgcc
     2341 cgccgcctcg cctacgtgct cgccggcgcg accgtgttcc tgcaacacac cccgacctcg
     2401 acccgcctga ccgccgacac cgagttcggc ccgctcgagt gggccggccc gctgtcgatg
     2461 ttcgcggtgt gcaacggcat gaccgtcggc ggcggccgcc cgctcgcgcc cgaggccagc
     2521 gtctccgacg gcctgttcga cgtgttcttc gtcaaggagg tcagcggcac cggcctcgcc
     2581 tcggtgctgc tgcagatggg ccgcggcctg cacct
//
LOCUS       DASWNJ010000671         1658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_118251, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000671 DASWNJ010000000
VERSION     DASWNJ010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118251"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..1114
                     /locus_tag="VGB85_11195"
     CDS             53..1114
                     /locus_tag="VGB85_11195"
                     /inference="COORDINATES: protein motif:HMM:NF021613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEY0134643.1"
                     /translation="MQAPAPEAVASLSEVVADPNRLFIAHACVRAVQEGRTRQGRPFL
                     DLSVADATRVLAGKIWDDAPEALEAGRAIVRGSIVKIMFRAELYQGALQLGVRRIRAM
                     QPGEVDVAAILGEGHGPVADVLCRSLVFDIETVPAQPRHELPAGVGKALDRFSGGDDG
                     AADLACSLSPLLARVVSLAFADGESPPDEAAVTVLAVPPEGRSSEQSDYPPWLRIVDE
                     RTLLQAFWILASQAEVVVSYNGRNFDVPFLVTRSLLHGVPARVDLLGKPYDLRPHLDL
                     YRVLQPGRALGPAGLDVLCWALGVESPKGTLDGSQVAPTYARGDIEAIAAYNRGDVKA
                     TVAVYQRVRDTILRFRPEW"
     gene            1160..>1658
                     /locus_tag="VGB85_11200"
     CDS             1160..>1658
                     /locus_tag="VGB85_11200"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY0134644.1"
                     /translation="MLVPRLIALLALFSTPLACGGGGAGTDSTDDGGDSDTEEPIEDP
                     EVFPGLRGEVEILIDKRGIPHIYAANDRDLFYAAGYQMATDRLFQIDLMRRRAHGRGA
                     EVLGESKIDEDKISRLFDFKRWGALDRDRFKAEAPADYALFTAWVAGVNKRIDEIAAG
                     TAPTPY"
BASE COUNT          216 a          630 c          564 g          248 t
ORIGIN      
        1 gtctgcctgt tcaggtgcat ccgcggcggg tgcggatatg ctcggccccc gcatgcaagc
       61 ccccgcgccc gaggccgtcg cgtcgctgtc ggaggtcgtc gccgatccga accggctgtt
      121 catcgcccac gcgtgcgtgc gtgcggtgca ggagggccgc acgcgccagg gccggccgtt
      181 cctcgacctc agcgtcgccg acgcgacgcg cgtgctcgcc ggcaagatct gggacgacgc
      241 ccccgaggcg ctcgaggccg gtcgcgcgat cgtccgcgga tcgatcgtca agatcatgtt
      301 ccgcgccgag ctgtatcagg gcgccctcca gctcggggtc cgcagaatcc gtgcgatgca
      361 gcccggcgag gtcgacgtcg ccgcgatcct cggtgagggc cacggcccgg tcgccgacgt
      421 gctgtgccga tcgctggtgt tcgacatcga gacggtgccg gcccagccgc gccacgagct
      481 gcccgcgggc gtcggcaagg ccctcgaccg cttctccggc ggcgacgacg gtgccgccga
      541 cctcgcgtgc agcctctcgc cgctgctcgc ccgcgtcgtc tcgctcgcgt tcgccgacgg
      601 tgaatcgccg cccgacgagg ccgccgtcac cgtgctcgcg gtgccgcccg agggtcgctc
      661 gagcgagcaa agcgactacc cgccgtggct gcggatcgtc gacgagcgca cgctcttgca
      721 ggcgttctgg atcctcgcct cgcaggccga ggtcgtcgtc agctacaacg gccgcaactt
      781 cgacgtgccc ttcctggtca cgcgcagcct cctccacggc gtgccggcgc gcgtcgacct
      841 gctcggcaag ccctacgacc tgcgcccgca cctcgacctc taccgcgtgc tccagccggg
      901 tcgcgcgctc gggccggccg gcctggacgt cctgtgctgg gcgctcggcg tcgagtcgcc
      961 caagggcacg ctcgacggca gccaggtcgc gccgacctac gcgcgcggcg acatcgaggc
     1021 gatcgccgcc tacaaccgcg gcgacgtcaa ggccacggtc gccgtgtacc agcgcgtgcg
     1081 cgacacgatc ctgcgctttc gtcccgagtg gtagcggatt tcttgtgctc ggccgggcac
     1141 ctgtgcttgg gtaccgggca tgctggtccc ccgcctgatc gccctcctcg cgctgttctc
     1201 gaccccgctc gcctgcggtg gcggcggagc cggcaccgat tcgaccgacg acggcggtga
     1261 cagcgacacc gaggagccga tcgaggaccc cgaggtgttc ccgggcctgc gcggtgaggt
     1321 cgagatcctg atcgacaagc gcggcatccc gcacatctac gccgccaacg accgcgacct
     1381 gttttacgcc gcgggctacc agatggcgac cgaccgcctg ttccagatcg acctcatgcg
     1441 ccgccgggcc cacgggcgcg gtgccgaggt gctcggcgag tcgaagatcg acgaggacaa
     1501 gatctcgcgg ctgttcgact tcaagcgctg gggcgcgctc gaccgcgacc gcttcaaggc
     1561 cgaggcgccc gccgactacg cgcttttcac cgcgtgggtc gccggcgtca acaagcgcat
     1621 cgacgagatc gccgccggca ccgcgccgac gccctacg
//
LOCUS       DASWNJ010000672         1532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_648049, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000672 DASWNJ010000000
VERSION     DASWNJ010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648049"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="VGB85_11205"
     CDS             <1..1136
                     /locus_tag="VGB85_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY0134645.1"
                     /translation="VQLPIQYAAGGEAIAAYLECMGEGRLVQSFKTHLTTASLGRTQI
                     AHHAIGLEDMISLYLKRLRQRAEEQLGAPIRAATIGRPVRFAGASTPEDDAVAAGRLM
                     TAAQQAGFEDARLELEPVAAAHHYERTLARPELALVADFGAGTTDFCLMRMGPTGQGS
                     TRAREIVGTGGVGAAGDDLDAALMENLVCPALGKGDTYVEMGRERAIPGSYFYKLSRW
                     HHLPFLGGSRVRAELERLHRGARHPERIAALMHIIATNSAFHLHKAVERTKVELSSRP
                     AARFDFSDGEVEIHAAVKRRDFERWIAPVTAAIAACLDDTLARAGVSAGEVDCVFMTG
                     GTAFVPVVRALFEQRFGAAKLRSGDELMSITSGLALRAAADPT"
     gene            1191..>1532
                     /locus_tag="VGB85_11210"
     CDS             1191..>1532
                     /locus_tag="VGB85_11210"
                     /inference="COORDINATES: protein motif:HMM:NF040011.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6151 family protein"
                     /protein_id="HEY0134646.1"
                     /translation="MPSEIPLRCACGTIRGTALQASGSTGSRIFCYCDDCQAFARFVD
                     HPGVMNEQGGTDIYQMAPGRLKITAGVDALRCVRLSEKGLYRWYCGECRTPIGNTKPP
                     VPFVGLIHSFMD"
BASE COUNT          215 a          510 c          575 g          232 t
ORIGIN      
        1 aggtgcagct gccgatccag tacgcggccg gtggtgaggc gatcgcggcg tacctcgagt
       61 gcatgggcga gggtcgcctc gtgcagtcgt tcaagaccca cctgacgacg gcctcgctcg
      121 ggcgcacgca gatcgctcac cacgcgatcg gcctcgagga catgatctcg ctgtatttga
      181 agcggctgcg ccagcgggcg gaggagcagc tgggcgcgcc gatccgggcg gcgacgatcg
      241 gcaggccggt gcgcttcgcc ggcgcgtcga cgccggagga cgacgcggtc gcggccgggc
      301 ggctgatgac ggccgcgcag caggccggct tcgaggacgc gcgcctcgag ctcgagccgg
      361 tcgccgcggc gcatcactac gagcgcacgc tggcgcgccc cgagctggcg ctcgtcgccg
      421 acttcggggc gggcacgacc gacttttgtc tcatgcgcat gggtccgacc gggcagggca
      481 gcacacgcgc gcgggagatc gtcgggaccg gcggcgtcgg tgcggcgggc gacgacctcg
      541 acgcggcgct gatggagaac ctcgtgtgcc cggcgctcgg caagggcgac acctacgtgg
      601 agatgggccg cgagcgggcg atccccgggt cgtattttta taagctgtcg aggtggcacc
      661 atttgccgtt cctcggcgga tcgcgcgtgc gcgccgagct cgagcggctg catcgcgggg
      721 cgcggcatcc ggagcggatc gcggcgttga tgcatatcat cgccacgaac tccgcgtttc
      781 acctgcacaa ggcggtcgag cgcaccaagg tcgagctgtc gagccgaccg gcggcgcgct
      841 tcgacttcag cgacggcgag gtcgagatcc acgccgccgt caagcgccgg gacttcgagc
      901 ggtggatcgc gccggtgacc gccgcgatcg cggcctgcct cgacgatacg ctggcgcggg
      961 ccggcgtgag cgcgggggag gtcgactgcg tgttcatgac cggggggacg gcgttcgtgc
     1021 cggtcgtgcg cgccctgttc gagcagcgct tcggcgccgc gaagctgcgc agcggcgacg
     1081 agctgatgtc gatcacgtcc gggctggcgt tgcgggcggc cgccgacccg acctgatccg
     1141 agcaaagacc cactcgacga gcgtgggctg cgctgttagg ttccgggccc atgccatccg
     1201 aaatcccgct ccgctgcgcc tgtgggacga tccgggggac cgcgctccag gcctcgggga
     1261 gcaccggttc gcggatcttc tgttactgcg acgactgcca ggccttcgcc cgcttcgtcg
     1321 accaccccgg ggtgatgaac gagcagggcg ggaccgacat ctatcagatg gcgccgggcc
     1381 gcctcaagat caccgccggc gtcgacgcgc tgcgctgcgt gcgtctgtcg gagaagggcc
     1441 tgtatcgctg gtactgcggc gagtgccgga cgccgatcgg caacaccaag ccgccggtgc
     1501 cgtttgtcgg gctgatccac tcgttcatgg at
//
LOCUS       DASWNJ010000673         6182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_577954, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000673 DASWNJ010000000
VERSION     DASWNJ010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6182
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577954"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..179)
                     /locus_tag="VGB85_11215"
     tRNA            complement(111..179)
                     /locus_tag="VGB85_11215"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(148..150),aa:Phe,seq:gaa)
     gene            complement(269..979)
                     /locus_tag="VGB85_11220"
     CDS             complement(269..979)
                     /locus_tag="VGB85_11220"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEY0134647.1"
                     /translation="MSSEPNRSLVSLLEELDRLPGVRGSLVATVDGAYQASERGAFDN
                     ATATDIAKTVRRMVVASATVGSAIEELLITFGSARMMIVPVREEATLTIMLERDSATP
                     LVRSTLQNRIGGLLQQLGSGSGSGESSVSNNVVAEPEDEVDRLMSGELGPVLRDVQAV
                     FTTYVVRGGRDSEEAGVMMREQMREWLLCCNPSPYTFPLLIDGLSQVLSDRQGERTTF
                     MNDVQAAMRNSKLWAGKR"
     gene            complement(976..2880)
                     /locus_tag="VGB85_11225"
     CDS             complement(976..2880)
                     /locus_tag="VGB85_11225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEY0134648.1"
                     /translation="MSPNPKSSTATVIHTLVVVGDQLLQAAIERQLQQVGHASASAAG
                     VREAIDRLRREPFDVVLCELHPDGSEGTELLQYMTSYYPSTPVVVICEALSPEFRGQY
                     EGGGAVRIVEKPINLGKLVEIIEACGPHKGFFGNQIEVEFFDYVQMVAVSARDKQLVV
                     TTRQGNGIIWFEHGDIVHAQFHDFRGEQAFYKMLAVGRGTYREVFFRPPPQRTIVRPS
                     MHLLMEAARQADEGILGTTETVEAEEEKKPKKKPPAPEPSESLSMMREEDMGEPQRAP
                     EPARTRAPMPKPERPTTPPRAAPATVAPSASTTAGRRPSESRTDGRLDARNDSSRSDG
                     RLDNRGDNRGAGDSQIDGGRPGDGRPGDIDMAGVADSAIARFDELTMPSDDDEVEADR
                     GRGGRGPADAQDGVPQRFRRVQTLVERVPDRGTPTSLPAVGSRITPVSMPAVGRATPT
                     SVPATPASSSRSTPTSMPATPASSSRATPTSMPATRTTPTSTPVPRPTGGGARPPLRP
                     DARPIKKAASVDLLDDPETRDLMLGQFWQFEGVNGVAIISSTGKVIAEDMRNNSTVVT
                     LAGFYMRGAARIARTLGYNVFDGVIARAANGQQMIMVGMGATSAVLSVAPGHDPEVIR
                     DAIMGVDTKR"
     gene            3267..3500
                     /locus_tag="VGB85_11230"
     CDS             3267..3500
                     /locus_tag="VGB85_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0134649.1"
                     /translation="MRLVLEDWNLNAAERLLCVEALATAGSIVEAAQLLGITRHALKR
                     RIIKHRIEWPRSSASAGDSATANTSASASITAE"
     gene            complement(4316..4558)
                     /locus_tag="VGB85_11235"
     CDS             complement(4316..4558)
                     /locus_tag="VGB85_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134650.1"
                     /translation="MTASIPLHIVVQQLLLGRTEGLAPQQLAAFISGWTSLLDLLKRP
                     ELCLPEASEEVQAALTALAGAIERAQDTVLGDDDDA"
     gene            4670..5572
                     /gene="epsC"
                     /locus_tag="VGB85_11240"
     CDS             4670..5572
                     /gene="epsC"
                     /locus_tag="VGB85_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HEY0134651.1"
                     /translation="MNEHERRLAQVVEGILDSYREHGNINHLDGSNLPARSEVSELLD
                     DLISVVFPGYFNGEQLDELTSRYFVGARCARILRRMEQVMARALAVDCARREAGTSDA
                     TSAHEHALELLQAIPTIREQVASDVQAALGGDPAACSGAEIIMSYPGVAAIAVHRIAH
                     YLYSRRVPLLPRMMSELVHSRTGIDIHPGATIGESFFIDHGTGVVIGETCRIGNRVKL
                     YQGVTLGAHSVKAREARGQRHPTIEDDVTVYAGATILGGDTVIGRGSTIGGNVWLTRS
                     VPPGTTVVLDTPSLRFFSLGDRQP"
     gene            complement(5656..>6182)
                     /locus_tag="VGB85_11245"
     CDS             complement(5656..>6182)
                     /locus_tag="VGB85_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134652.1"
                     /translation="QTEFMRCGKLFDLSGVRIIDAQVIYHQKEPRSLTAAMRFYRDKE
                     FKDAHGAEADTVATVEVFAGQLERYGELALEVDALHEVSNAVNNGYVDLSRRFSWRDN
                     EPVFNFGKLKGKSLRWAASEPTERTYMRWILDGNFEEDTKLIIREALAGRIRQRVRTA
                     RPAPESSDAAAEAS"
BASE COUNT          906 a         2028 c         2216 g         1032 t
ORIGIN      
        1 actgccgcgg cccgaagacc ctctacagtt cgatgtgcac tccctctgcg caaaacggcg
       61 ccccgcttta gcggagcgcc gtttgaattg ctctgggatc tgcaacgcag tggctaggga
      121 cggaatcgaa ccgccgacac gcggattttc agtccactgc tctaccgact gagctacctg
      181 ggcgcgggga cgaagtggta cgagattccc gggccggcgt caagcgccgc gtgatcgcgg
      241 actccacagg ctctgtgggc atttgatctc agcgcttgcc ggcccacagc ttcgagttgc
      301 gcatggcggc ctggacgtcg ttcatgaagg tcgtgcgctc gccctggcgg tcgctgagga
      361 cctgcgagag gccatcgatc aacagcggga aggtgtaggg cgaggggttg cagcacagca
      421 gccactcgcg catctgctcc cgcatcatca cgccggcctc ttcgctgtcg cggccgccgc
      481 ggaccacgta ggtggtgaac acggcctgga cgtcgcgcag gaccgggccg agctcgccgg
      541 acatcaggcg gtcgacctcg tcctcgggct cggcgacgac gttgttggag acgctgctct
      601 cgccggagcc ggagccggag ccgagctgct gcagcaggcc gccgatgcgg ttttgcaggg
      661 tcgaccggac cagcggggtc gccgagtcgc gctcgagcat gatcgtgagg gtcgcctcct
      721 cgcggaccgg cacgatcatc atgcgggcgc tgccgaaggt gatcagcagc tcttcgatcg
      781 ccgagccgac ggtcgccgag gcgacgacca tgcggcgcac ggtcttggcg atgtcggtcg
      841 cggtggcgtt gtcgaaggcg ccgcgctcgc tggcctgata ggcgccatcg acggtggcga
      901 ccagcgagcc gcgcacgccc gggagccggt cgagctcctc cagcagcgag actagcgaac
      961 ggttcggctc ggagctcatc gcttggtgtc gactcccatg atcgcgtcgc ggatgacctc
     1021 ggggtcgtgg cccggtgcga cggagaggac cgccgaggtc gcccccatcc ctaccatgat
     1081 catctgctgg ccgttggccg cgcgggcgat caccccgtcg aagacgttgt aaccgagggt
     1141 gcgggcgatg cgggccgcgc cgcgcatgta aaagccggcc agagtcacga cggtgctgtt
     1201 attgcgcatg tcctcggcga tcaccttgcc ggtgctcgag atgatcgcca cgccgttgac
     1261 gccctcgaac tgccaaaact ggccgagcat gaggtcgcgc gtctcggggt cgtcgagcag
     1321 atcgacgctg gcggccttct tgatcgggcg ggcgtcgggg cgcagcggcg ggcgggcgcc
     1381 gccgccggtc ggccgcggca ccggggtcga ggtcggggtc gtccgcgtcg ccggcatcga
     1441 ggtcggggtc gcgcgcgatg atgaagcggg cgtcgccggc atcgaggtcg gggtgctgcg
     1501 cgatgatgaa gcgggcgtcg ccggcacgga ggtcggggtg gcgcggccga cggcgggcat
     1561 cgacaccggg gtgatgcggc tgccgacggc cggcagcgag gtcggggtgc cgcggtcggg
     1621 cacgcgctcg acgagggtct ggacccggcg gaagcgctgg ggcacgccgt cctgggcgtc
     1681 ggcgggaccg cgtccgccgc gaccgcggtc ggcctcgacc tcgtcgtcgt ccgacggcat
     1741 cgtcagctcg tcgaagcggg cgatcgccga gtcggcgacg ccggccatgt cgatgtcgcc
     1801 ggggcgcccg tcaccggggc ggccaccgtc gatctggctg tccccggcgc cgcggttgtc
     1861 accacggttg tcgaggcggc cgtccgagcg gctgctgtcg ttgcgagcgt cgaggcggcc
     1921 gtcggtgcgg ctctcgctcg ggcggcggcc ggcggtcgtc gaggccgagg gcgcgacggt
     1981 ggccggcgct gcacgcggcg gcgtggtcgg gcgctcgggc ttgggcatcg gggcccgggt
     2041 gcgcgccggc tccggggcgc gctgcggctc gcccatgtct tcctcgcgca tcatcgacag
     2101 cgactcgctc ggctcgggcg cgggcggctt cttcttcggc ttcttctctt cctcggcctc
     2161 gacggtctcg gtggtgccga ggatgccctc gtcggcctga cgcgcggcct ccatcagcag
     2221 gtgcatgctc ggccgaacga tcgttcgctg cggcgggggc cggaagaaca cctcgcggta
     2281 ggtgccgcgg ccgacggcga gcatcttgta aaaggcctgc tcgccgcgaa agtcgtggaa
     2341 ctgggcgtgc acgatgtcgc cgtgctcgaa ccagatgatg ccgttgccct ggcgggtcgt
     2401 gacgaccagc tgcttgtcgc gcgccgagac ggcgaccatc tgcacgtagt cgaagaactc
     2461 gacctcgatc tggttgccga agaacccctt gtgcggtccg caggcctcga tgatctcgac
     2521 gagcttgccg aggttgatcg gcttctcgac gatgcgcacc gcgccgccgc cctcgtactg
     2581 gccgcggaac tccgggctca gcgcctcgca gatgaccacc accggcgtcg acgggtagta
     2641 gctggtcatg tactgcagca gctcggtgcc ctcgctgccg tcggggtgca gctcgcacag
     2701 cacgacgtcg aagggctcgc ggcgtaagcg atcgatcgcc tcacgcacgc ccgcggccga
     2761 cgccgaggcg tgaccgacct gttgcagctg tcgctcgatc gccgcctgca acagctgatc
     2821 gcccaccacg acgagcgtgt gaatcacggt ggcggtgctg gacttcgggt ttggcgacac
     2881 gcggcttcct atcttatctc gcgctcaggt gcgacgggcc ggattagaac ctaggagtga
     2941 cacccgcggg gtctcgagca ctctggaggt gcatggacga tccaccatga aggggtccgg
     3001 acgctctcgc agacgtgagc tcgttttgtt ggattgggtt tgaaggtcac gatgcgcaca
     3061 tgggcgcgaa tgtatgacgt actaccgcca tcgtggtgtg gcttcaaggg cgtctacgcg
     3121 caggtacaat ggtttttgta cccatgtgaa cgaagttact gaacaaaact actaaatctt
     3181 ctttcgcgtt acaatcgagt tcttttggcg aggcggctta cagtttcgtc ctttcatgtc
     3241 tccgaccttt ctcctattct gcccccatgc gcctcgtcct cgaagactgg aacctcaacg
     3301 ccgccgaacg actgctctgc gtggaagccc ttgcgaccgc cggcagcatc gtcgaagccg
     3361 ctcaactcct cggcatcacg cgtcacgcgc tcaagcgccg catcatcaag catcgcatcg
     3421 agtggccccg ttcgagcgcc tccgcgggtg actcggccac ggccaacacc agcgcttccg
     3481 cctcgatcac cgccgagtaa catccaccac tgacgagccc cgacggggcc gcaaacaccg
     3541 ccagcgcgcg tccgggcaac ctctcccggg tgcgcgctgt tgtttttggt tgatcacggc
     3601 ctcgggccca atcgaacatt gttctcttcg gccggtcgcg ggcccgtccg cccgtgcggg
     3661 cgaccggggc gcggtggccg ggtcggccgg cgcgcggtcg aggctccgga gctgcgcgac
     3721 caggcgctcg ccgcgaacgg gccggcgggc ggcgcggtcg cggcagttcg ccgacagacg
     3781 ggtcgtggca cccggctgcc gatcgccgga cgaacggccg gacagcgccg cggacacgct
     3841 ccgagatctc tccggagatc tcgtactttc gcacgtgctc gcaagcgcgc gctccgtcct
     3901 gaaccgtgtt tgtctgtttc cgccatccgc gcccttgacc gcacggctcg gatctgcacg
     3961 aaatagatga tagttttgat aggttttcag cgagcgcgag gtcgcacaat ttaacgcgaa
     4021 tttagccgtg gaacgcgcat ctacgcgccg ggcgaaaaca gtttcagtag tattttgtgc
     4081 actaaaattt aagtaagcca gcgtacacat agtacgcatg atgaagctgg ccaaggtacc
     4141 gtgcgaggcg gtgcgggccg ccgtctctgg tgggagtggg cgttttgcgc caagatcacg
     4201 gccctggatc tggcgatcca gggccgaact tggtgcgacc ggtggtgcgc tggaagcgcg
     4261 agagcgcgca ccaggctggg ggatctctca gaccccggga gcccccggat cggggtcagg
     4321 cgtcgtcgtc gtcgccgagg acggtgtcct gggcgcgctc gatggccccg gcgagggcgg
     4381 tcaaggcggc ctggacctcc tcgctggcct cggggaggca cagctcgggg cgcttgagca
     4441 ggtcgagcag gctggtccat ccgctgatga aggcggcgag ctgttgcggc gccagcccct
     4501 cggtgcgccc gaggagtagc tgctggacca cgatgtggag ggggatggac gccgtcacgc
     4561 cgaaagaagc ttagcccggg cgcgcgaggc ggccaaaaaa ccccgatctg gcgctcgccg
     4621 cggccggggc ggatggtgga tcgcgcgggc gatgttctat ggttgctcgg tgaacgagca
     4681 cgaacgacgg ctggcgcagg tggtggaggg gatcctcgac tcgtaccggg agcacggcaa
     4741 catcaaccac ctcgacggca gcaacttgcc ggcgcgcagc gaggtttcgg agctgctcga
     4801 cgacctgatc tcggtggtgt tccccggcta cttcaacggc gagcagctcg acgagctgac
     4861 gtcgcggtac ttcgtcgggg cccgctgcgc ccgcatcctg cggcggatgg agcaggtgat
     4921 ggcgcgcgcg ctggccgtcg actgcgcgcg ccgcgaggcc ggcacgtcgg acgcgacctc
     4981 ggcccacgag cacgcgctcg agctgctgca ggcgatcccg acgatccgcg agcaggtcgc
     5041 gtcggacgtg caggcggcgc tcggcgggga cccagcggcg tgcagcggcg cggagatcat
     5101 catgagctac cccggcgtcg ccgcgatcgc ggtgcatcgc atcgcgcatt acctgtactc
     5161 gcggcgcgtg ccgctgctgc cgcgcatgat gtcggagctg gtccacagcc gcaccggcat
     5221 cgacatccac cccggcgcga cgatcggcga gagcttcttc atcgaccacg ggaccggcgt
     5281 cgtcatcggc gagacgtgca ggatcgggaa ccgcgtcaag ctgtatcagg gcgtcaccct
     5341 cggcgcgcac tcggtgaagg cgcgcgaggc ccggggccag cgtcatccca cgatcgagga
     5401 cgatgtcacg gtgtacgccg gcgcgacgat cctcggcggc gacacggtga tcggccgcgg
     5461 ctcgacgatc ggcggcaacg tgtggctgac gcgcagcgtg ccgccgggca ccacggtggt
     5521 gctcgacacg ccgtcgctgc gcttcttcag cctcggcgac cgccagccct gacgatcagg
     5581 acgcctcggc cgtcaggacg cctccgccgc ggctgtcagg acgcctcagc cggggccgtc
     5641 aggacgccgc ggccgtcagg acgcctcggc cgccgcgtcg gacgactcgg gggcggggcg
     5701 ggcggtgcgg acgcgctggc ggatgcggcc tgcgagcgct tcgcggatga tcagcttggt
     5761 gtcctcctcg aagttgccgt cgaggatcca ccgcatgtac gtgcgctcgg tcggctcgct
     5821 ggcggcccag cgcaggctct tgcccttgag cttgccgaag ttgaacaccg gctcgttgtc
     5881 ccgccacgag aagcggcggc tgaggtcgac gtagccgttg ttgacggcgt tggagacctc
     5941 gtgcagggcg tcgacctcga gcgcgagctc gccgtagcgc tcgagctggc cggcgaacac
     6001 ctcgacggtg gcgacggtgt cggcctcggc gccgtgggcg tccttgaatt ccttgtcacg
     6061 atagaagcgc atcgcggcgg tgaggctgcg cggctccttt tggtggtaga tgacctgggc
     6121 gtcgatgatg cggacgccgc tgaggtcgaa cagcttgccg cagcgcatga actcggtctg
     6181 ga
//
LOCUS       DASWNJ010000674         1655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_364885, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000674 DASWNJ010000000
VERSION     DASWNJ010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364885"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1654)
                     /locus_tag="VGB85_11250"
     CDS             complement(167..1654)
                     /locus_tag="VGB85_11250"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019691833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEY0134653.1"
                     /translation="MAGTLQDTFAAVGVREIAVHESAGEAVDRVTDLYEAATARLVEA
                     FQRFTRDGRDPGPIDARYPFVGVRVEAGDLNLDGRLAYGALHDPGTYGTTLTRPRLFG
                     DYYRTQLGILLGNHPVRLVTGVSTRPIPLPFIVEAMTSETPKERLHEVQHLFPMPDLS
                     FTDDSIANGTWRAGRAEPWPLALFTAERVDYSLHRLHHYTGTRPEFFQRFVLLTNYQR
                     YVDHFVAYGRREVAEGEEYDRFVEPGDVVVPSPRLRPTDQPAGTPPAHLPQMPAYHLT
                     RPDGRGVTMINIGVGPSNARTITDHVAVLRPHCWLMLGHCAGLRRSQMLGDYVLAHGY
                     VREDGVLDQEVPLFVPVPPIAEVQVALQEAVVNVTGLKGHDVKTRMRTGTVATTGNRN
                     WELRYDELFERLNQSRAIAVDMESATIAANGFRFRVPYGTLLCVSDKPLHGELKLRGM
                     ANRFYRERIDQHLQIGLEAVRLLREQGPDRLHSRKLRSFDEPAFR"
BASE COUNT          222 a          561 c          626 g          246 t
ORIGIN      
        1 ccgcggacga gcgcggcacc acctcgatcg agtacgcgct gctggccgcg gtcgccgcga
       61 tcacggcgat cggcggcctg caagccttcg cgggcgagac cggcgcgctg tggggcgtgg
      121 tcgatggcgc caaccaagcg atcgcggccg tgttgggccg ctgatgtcac cggaacgcgg
      181 gctcgtcgaa gctcctgagc ttgcgcgagt ggaggcggtc cgggccctgc tcgcggagca
      241 ggcggacggc ctcgagcccg atctggaggt gctggtcgat ccgctcgcgg tagaaccggt
      301 tggccatgcc gcgcagcttc agctcgccgt gcagcggctt gtcggagacg cacagcaagg
      361 tgccgtaggg gacgcggaag cggaagccgt tggcggcgat ggtggcgctc tccatgtcga
      421 ccgcgatggc gcggctctgg ttcagccgct cgaacagctc gtcgtagcgc agctcccagt
      481 tgcggttgcc ggtggtggcc accgtgccgg tgcgcatccg ggtcttcacg tcgtggccct
      541 tgaggccggt gacgttcacg accgcctcct ggagcgccac ctgcacctcc gcgatcggcg
      601 ggacggggac gaacagcggc acctcctggt cgagcacgcc gtcctcgcgc acgtagccgt
      661 gggccaggac gtagtcgccc agcatctgcg agcgccgcag cccggcgcag tggccgagca
      721 tcagccagca gtgcgggcgg agcacggcca cgtggtcggt gatcgtgcgc gcgttgctgg
      781 gcccgacgcc gatgttgatc atggtcacgc cgcgcccgtc cggccgggtc aggtgatagg
      841 caggcatctg cggcaggtgg gcgggcggcg tcccggccgg ctggtcggtc gggcgcagac
      901 gcgggctcgg caccaccacg tcgccgggct cgacgaagcg gtcgtactcc tccccttccg
      961 cgacctcgcg ccggccgtag gccacgaagt ggtcgacgta gcgctggtag ttcgtgagca
     1021 ggacgaagcg ctggaagaac tcgggccgtg tgccggtata atggtgcagc cggtgcaggc
     1081 tgtagtcgac gcgctcggcc gtgaacaacg ccagcggcca cggctcggcg cggccggcgc
     1141 gccaggtgcc gttcgcgatc gagtcgtcgg tgaagctgag atccggcatc gggaacaggt
     1201 gctggacctc gtgcagccgc tccttcgggg tctcgctggt catcgcctcg acgatgaacg
     1261 gcagcgggat cgggcgggtg ctcaccccgg tcacgagccg gaccgggtgg ttgccgagca
     1321 ggatgccgag ctgggtgcgg tagtagtcgc cgaacagccg cggccgggtg agcgtcgtgc
     1381 cgtaggtgcc ggggtcgtgc agcgcgccgt aggccagccg cccgtcgagg ttcaggtcgc
     1441 cggcctcgac ccgcaccccc acgaacgggt agcgcgcatc gatcggcccg ggatcgcggc
     1501 cgtcgcgcgt gaaccgctgg aacgcctcga ccagtcgcgc cgtcgccgcc tcgtagaggt
     1561 ccgtgacccg gtcgacggcc tcgcccgcgc tctcgtgcac cgcgatctcg cgcacgccga
     1621 ccgcggcgaa ggtgtcctgc aacgttccgg ccatg
//
LOCUS       DASWNJ010000675         2135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_260633, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000675 DASWNJ010000000
VERSION     DASWNJ010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260633"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VGB85_11255"
     CDS             complement(<1..744)
                     /locus_tag="VGB85_11255"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEY0134654.1"
                     /translation="MAQAQQDPPRAEVDEADRAMEQVMRFVGILRRRWLVVAVCTVVA
                     VAGSFVAISVLQPRWVASATVVLHLSGPQVLDKMKGVSDDNEGRLFAYKEYYQTQREI
                     IGSRVVAERALGALGLAQDPVFLGVDGIGSEPERLARMQKIDPVDRLREMVFVEEVRG
                     SRLLRIAAEYPDPQVAADIANAISDAYLAHIQQSRTRTGVAAKDNMGVERVKSLAALQ
                     AAEKALADFKQAHGISSISLADRQNVITAA"
     gene            complement(861..1166)
                     /locus_tag="VGB85_11260"
     CDS             complement(861..1166)
                     /locus_tag="VGB85_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134655.1"
                     /translation="MDDTYLIGFATAIAVAGAIFVASRRAGKLGPKLRDALEQAGGEL
                     TLPALTAAVGFKDSFINRGKVIQAIAPLVRSGAVVETDDPAATAKTRLELKRFRLQR"
     gene            complement(1203..1949)
                     /locus_tag="VGB85_11265"
     CDS             complement(1203..1949)
                     /locus_tag="VGB85_11265"
                     /inference="COORDINATES: protein motif:HMM:NF028481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0134656.1"
                     /translation="MPTATALPTVPSLVLCELDRTDLPGLESYSPFCLKVHRALKSAG
                     LPYTSRHGAHPGAHGDVSKNGQVPVLLLDGEAIDDSTVILARLAALQPTPAWAAVDPQ
                     LHAEALLWEELADVSLGGFVMAARWADDRNWPAVQGALFGAAPWPVRRFIAPATRRRI
                     TATLVARDVWRAGPDACWRRFDTLLDSLERLAPAAGFWLGDHLTVADLGLFAHLHSLR
                     TPLTPWQRARVEARPALLAWLDRVDLATRG"
     gene            2000..>2135
                     /locus_tag="VGB85_11270"
     CDS             2000..>2135
                     /locus_tag="VGB85_11270"
                     /inference="COORDINATES: protein motif:HMM:NF019884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEY0134657.1"
                     /translation="MRRSERLFAIAEHLRGRRSGVTAAALAERFGVTLRTMYRDLESL
                     R"
BASE COUNT          301 a          735 c          807 g          292 t
ORIGIN      
        1 cgccgcggtg atgacgttct ggcggtcggc gagggagatc gagctgatgc cgtgggcctg
       61 cttgaagtcg gcgagcgcct tctcggccgc ctgcagggcc gcgagcgact tgacgcgctc
      121 gacgcccatg ttgtccttgg cggcgacgcc ggtgcgcgtg cggctctgct ggatgtgcgc
      181 gagataggcg tccgagatcg cgttggcgat gtccgcggcg acctgcggat cggggtactc
      241 ggcggcgatc cgcagcagcc gcgagccgcg gacctcctcg acgaacacca tctcgcgcag
      301 gcgatcgacc gggtcgatct tctgcatgcg agcgaggcgc tccggctccg agccgatgcc
      361 gtcgacgccg aggaacacgg ggtcctgcgc gagcccgagt gccccgagcg cacgctcggc
      421 gaccacccga ctgccgatga tctcgcgctg ggtctggtag tactccttgt aggcgaacag
      481 ccggccctcg ttgtcgtccg agacgccctt catcttgtcg agcacctgcg ggcccgagag
      541 gtgcaacacg accgtcgcgc tcgccaccca gcgcggctgc aacaccgaga tcgcgacgaa
      601 gctgcccgcc accgccacca ccgtgcacac cgcgacgacg agccagcgcc ggcgcaggat
      661 gccgacgaag cgcatgacct gctccatcgc cctgtcggcc tcgtccacct ccgcccgggg
      721 cgggtcttgc tgcgcctgcg ccatcgccga ggggtcagct taggccgacc ggcgacaaac
      781 gccaagcaac cggcgcgcgc ccgccccggg ccgcgcgtgc gctatttgca tcatccgcgg
      841 tcgccctggc cgccgggtgc tcagcgttgc aggcggaagc gcttgagctc caggcgtgtc
      901 ttcgcggtcg cggcggggtc gtcggtctcg acgacagcgc cgctgcggac cagcggcgcg
      961 atcgcctgga tgaccttgcc gcggttgatg aacgagtcct tgaagccgac cgcggcggtc
     1021 agggccggca gcgtcagctc gccgccggcc tgctcgagcg cgtcgcgcag cttgggcccc
     1081 agcttcccgg cgcgccggct ggcgacgaag attgcgccgg cgacagcgat cgcggtggcg
     1141 aagccgatca ggtaggtgtc gtccatcgag tggcctcctt gcgcggccga ggatgacgca
     1201 ggtcagccgc gggtcgcaag atcgacgcgg tcgagccagg cgagcagggc cgggcgagcc
     1261 tcgacacggg cgcgctgcca cggggtcagc ggggtgcgca ggctgtgcag gtgggcgaac
     1321 aggccgaggt cggcgacggt caggtggtcg ccgagccaga agcctgcggc cggggcgagg
     1381 cgctcgagcg agtcgagcaa ggtgtcgaag cgccgccagc aggcgtcggg cccggcgcgc
     1441 cagacgtcgc gggcgaccag ggtcgcggtg atccggcggc gggtcgccgg ggcgatgaag
     1501 cggcgcaccg gccacggggc ggcgccgaac agggcgccct gcaccgcagg ccagttgcgg
     1561 tcgtcggccc agcgcgcggc catgacgaag ccgccgaggc tgacgtcggc cagctcctcc
     1621 cacagcaggg cctcggcgtg cagctgcggg tcgacggcgg cccacgcggg ggtcggctgc
     1681 aaggcggcga gccgcgcgag gatcacggtc gagtcgtcga tcgcctcgcc gtcgagcaac
     1741 aacaccggga cctggccgtt cttgctgacg tcgccgtgcg cccccgggtg cgcgccgtga
     1801 cgcgaggtgt acggcagacc cgcagacttc aacgcgcggt gcaccttgag gcaaaacggc
     1861 gagtaactct cgaggcccgg gaggtcggtg cggtcgagct cgcacaggac gaggctgggg
     1921 acggtgggca gagcggtcgc ggtgggcatg cccgacgaga tatcctggcg ctctgtcagc
     1981 attgtgtcat gtttgagcga tgcgtcgcag cgagcgcctg ttcgccatcg ccgagcacct
     2041 gcgggggcgt cggtccgggg tcactgccgc ggcgctggcc gagcgcttcg gcgtcacgct
     2101 gcgcacgatg taccgcgacc tcgagagcct gcgga
//
LOCUS       DASWNJ010000676        17856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_153671, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000676 DASWNJ010000000
VERSION     DASWNJ010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 17856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17856
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153671"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2615
                     /locus_tag="VGB85_11275"
     CDS             <1..2615
                     /locus_tag="VGB85_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134658.1"
                     /translation="SPPLVLALDDLHCADPAALHLIELILSDPAGGHLLVLASYRDAE
                     LGDAHPLRLTQDRLRRLGVPVYALPLHPLADDEVTQLVADALGCEVDEAAPLAARLDA
                     HAHGNAAMLRHLLLGLRREGQLTFDSASGRWQWNTSTAIGSGNPGNASSPGNVAAFVA
                     ARLRRLPSGVQRVLAIAALAGRAIDLPLLSAVDRRGPLATTDALRDALREGLLVPSDS
                     AVHSLQENPGTSPGTPRVTYQPLHDGIALAAADLLSADERAEIHLQLGRNLLRQQSPD
                     DDDLFALVDHLDRGAACMTELAERERLARLHLEACRRARIATAYAAAVRHARAGLVTL
                     AAIVAEPDEHVMLREALPSASDLKIVPDALWQRYPDHTFKLMREWMHAEALRGDLDAA
                     DALFDPLVRRSTHELARADLYRLKAQLDTYHGRPHDAMAAGLAGLYGLGVELLPRPEP
                     AEIEAEHLALTALLADVEVIKLIAGPVCTDPRVSATAELLSATGPAAMFADTRLAYHV
                     FIRLVRLFIEHGPTRDTAYGLAGYGLYLAGARRDTARAFEFGQYALKMRDRFADPSLG
                     ARIFHIVGGLITPWTQPFAVAAAILEQGYAIGVQTGDFAAATYNTTTLVLVLAARRHS
                     LHAVRATAEQLLVEARPMLEVYGSSIIRCSIRMSRCLAGELTAPTPGNGPRWLAEVFL
                     RDFERDGSPLTLLYLHIFAISVLYHFGLHDPADLPRLPPSAAERLGVLVTPMAADYFF
                     YNVLHACAAPTPATIHPKIEADIAELRKLASSCPANFEARAHLATAEYERVRGNDDAA
                     ESAFKLAIRSARKQGEAGTEALACELAGRHAASIGDDIVGTMYLRAAAEAYQRWGAPA
                     IARRLAHAVEARPD"
     gene            2737..4659
                     /locus_tag="VGB85_11280"
     CDS             2737..4659
                     /locus_tag="VGB85_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0134659.1"
                     /translation="MPSPSHLTDEKHVLHTLLAALFDADELRVFLHGFRAPEDLGRSL
                     PGGIASHDQLIGAAVQALQRRGLLDQEFFGALVRARPRRAREICTAARTLGVELPDAV
                     MAAAQTGDELDALLRDQADSHFGERLLSMIGHLPRTHPLVHRAHLMLAELRQQRRDSA
                     DLDVHERVAASVFDLALEVASTRTPSPRQTEDDDSGTSAQMLHAAVIAAVQENDGERA
                     VKRLLDLTRLAGQRSAMANRATLLAATIHDLRRRFRYISMPQEVRNEWLNTLSQILEL
                     ADDTLASSADADRSSVAPKPFVETDSGSLLHARQAFSLARQGARDAALAPITVHLGGL
                     SKVHGGRHKPFVLRDVSLDLRPGEITGVVGPNGSGKTTLLRIIAGETSHDSGTLAYPR
                     LDPRARAGRPNWSQVRPLIGYVAQRPPRWPGRLAENLHRWAALHGIYGDANREEVEFY
                     LHRLDLERFRDATWTEISGGYQMRFELARVLCAAPQLLVLDEPLAPLDLATQDLYLQD
                     LRDIASAPSRPIAVIVSSQHIYEIESIADHMLSLGEDGSVQYSGPARAILHPEDDRIF
                     EIEADLHPHHLDALRGAKLVRDSRRRGRRLLLSASATVSGADILDWLRHHGIPLVLFQ
                     DISDSCARLAKWNFAD"
     gene            5334..5987
                     /locus_tag="VGB85_11285"
     CDS             5334..5987
                     /locus_tag="VGB85_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134660.1"
                     /translation="MEVLQEGIHNVLGQKCIDEDCLASAERIGRFQTGPWTATSFLLP
                     GRREHLYGLLICLLLTVLGSALKNVHWRAITALSLILFFLWLLLPLGAFLGNLPANNR
                     TYSGTALLIFSLATFTVLHTVFFRKRRGAFSIDISLAASYITLPILPALMIGTLIRPE
                     GTSDEDILSIISNVIVMDVDFAELSAFTWVMLISPAWTLAFAAFLEPLARRWAHLPR"
     gene            6174..6971
                     /locus_tag="VGB85_11290"
     CDS             6174..6971
                     /locus_tag="VGB85_11290"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0134661.1"
                     /translation="MMIAGYNHDELLQEVLVRPREAHVPYADLREVVRPGWHQIVTPS
                     FFGGGLNGVDLAVLDHLDHATADAVIDATIAEYDALGIRFRWEIGPDSRPRDLAARLE
                     ARGLTSKRVLLLAAAIADLDIEAPPDVVVEPVGEHNLASFVSVVAAGWGMVPDPLIAY
                     ERAVVAHRSTNSTTLHQSFLATIDGEPAGAANAVCFPRSLYLMGAVVLPAHRGRGVYR
                     SLIAARLHLARARGVGLATSQALADTSAPILARMGFVTVAEQLSFAR"
     gene            7051..9318
                     /locus_tag="VGB85_11295"
     CDS             7051..9318
                     /locus_tag="VGB85_11295"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline ceramidase"
                     /protein_id="HEY0134662.1"
                     /translation="MRRALVPLLSAAALHCGDATSTSDSAGATTTATTTATTTTTAPT
                     EPTPTTGGTASASAPTTSETTSGASDSASDDSGPPADECATSPHYRVGAGIADVTGPS
                     AELVMMGYSQPTQTAEGISTRLWSRAFVVESPCSGGRIVFVSADLGQVFQSVHLEVLE
                     RLEARFGPGLYTRDNVALSATHTHSGLAGFSHYTLYNLAALGFDPDNFEAVVVGIVES
                     IARAHDNLGEGVVKIAQGPLQDATWNRSPQAYANNPDPERAAYEAEHARDVNTRMTVL
                     RLERTAAGATDGDPIAAIAWFSSHATSVSNANHLVSSDHKGLASLDFERSMGADYMSP
                     APFVAAFAQSDAGDVTPNEVIVGEPKNTVKRPCDGSPDPECTDLANAAIHADIQYARA
                     RQLFDEATELLRGPVDGRLAFFDMENVTVADEFTGAGEKHTCSAAIGASLAAGAEDGV
                     GVEFIQEGISLGTIPLFSLVPEDQVCHAEKTILLPTGRMQPHPWTPPIVPAMVLRLGN
                     LAIIPTPFEVTTISGDRLRATVLDRLAGTGVDRAVIAGLSNGYASYVTTREEYKAQHY
                     EGASTLFGPWTLAAWQQNFAGVAEALRDGTDAVHTGVPVDLSDAQMVETLPVVADSPC
                     AFPDPALCIGEKFGDVVQAPPATVEAGAHVVVRFRGAHPRNDLRTGTGYLEIQRKLGD
                     QWQTIARDWDPETTFRWQRTGGGLSPTSEVTIDWRVPLDAVPGEHRVVYHGDARTLLG
                     VITPISGTSPSFTVQ"
     gene            9362..10321
                     /locus_tag="VGB85_11300"
     CDS             9362..10321
                     /locus_tag="VGB85_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134663.1"
                     /translation="MRLPGLALLLPLAACFSPNPAFVPTTDGTGGTSTGTTATTAVDS
                     TDSDPTTTTTTGPDTTGSVTIEPLTSTSEPLTSTSEPSTSEPSTGEPLTSSSSSTGQP
                     DPPNCELDKADDDPVRVVRDLANNAKEITQCDEDTPTTWKGALTVQDGKLTMYLNDQC
                     NGGGPGLLVGEAWPASNNWTYPCVQTRVYWKKVQGQCRISALRVVELKEPMMDPAPPI
                     ILASLSHPSEPAEFQMWPALIASGMPCGCPPDLPDCCDPDPGQYELDFDDNVVIDSTL
                     EKTFPRFGSVYRLKNIQSYIGAGCQNDPGSDVHIDWFLERLPP"
     gene            complement(10322..11566)
                     /locus_tag="VGB85_11305"
     CDS             complement(10322..11566)
                     /locus_tag="VGB85_11305"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY0134664.1"
                     /translation="MQIDPKHFQVRREQQLRDEVVGDFGVRKALAKIREVRGGKGYGY
                     RRGLLSSTLRLTRSMSPEIADTVAQCKALIGHEGPVEVYVTPEHQFNATCYREVRGPT
                     VLTLSSRLLEAFTPAELRFVIGHELGHAAFDHYGLPMPVTAMVEELGIPFVSRHVSLK
                     LYAWCRSAELSADRIGLLCAQDPQAAASGFFKLASGMSSPRVVPDLETFARQVESLAA
                     APEAREKEYDEDTLDCFSTHPYNPVRVRAVWAFSRSKPFLDAMGKSSLDAWTLDEVET
                     MVDRDMSLMEPTYLQEKSQSSDTLRRLLYTAGVAVAAASGGVEASELDAIGRLLGSDH
                     ADAPTDVDKVLRDLDGRIRDALEVPLLYRAQLVQHLTIVAAADGHVSDDELHVMEDIA
                     RRLEVGPGVVHETIRGAMHPID"
     gene            complement(11644..12084)
                     /locus_tag="VGB85_11310"
     CDS             complement(11644..12084)
                     /locus_tag="VGB85_11310"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEY0134665.1"
                     /translation="MGLLSKLTGKVTPQKKPADDSLLLHAMLLMCGADGSFDEQEIPT
                     VEAYFNSLPEFEGKDFGTVYQEAAKVLKRFPNLKDSVKALGDLSTQTVKNKCYLRAAD
                     IAMASGDVDEAEDAMLDAMQRVLQVEDALATKILEVLTLKYARS"
     gene            12508..13578
                     /locus_tag="VGB85_11315"
     CDS             12508..13578
                     /locus_tag="VGB85_11315"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEY0134666.1"
                     /translation="MPLPTEPIGSIPRPPALIAAVAAMRPGETPDAHLDGLYDAAVRD
                     TIARFEATGSPVVTDGEQRKYHNFWTYCVHGTTNTAPDGFKIPFAAGHTRRMPRLTSG
                     PFKYMRHADSYLDLALRHALRPVKQAVISPSALSLMYPPEELAGYPRAQFIDDLLDEH
                     VTEVRACLQKGAHAVQIDFTEGRFAVKLDPSGDLLHRYIDLNNLALARFSADDRGRLG
                     VHTCPGGDRDSTHSADVDYAELLPALFELEVGNFYVALAGEPDRVRVLQLLREHLRPH
                     QRVFVGVVAPIDPRVETPEEVRDRVLEAARFIPVEQLGTTDDCGFAPFCDDTSTSRDT
                     AFAKIRARVLGTALASRALGVD"
     gene            13578..17339
                     /locus_tag="VGB85_11320"
     CDS             13578..17339
                     /locus_tag="VGB85_11320"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0134667.1"
                     /translation="MADDEEKQLRSVALQNANAILHTRARAERELAKERERLRITLAS
                     IGDAVISTDVDGRVTFLNRVAEHLTGWTQADAAGRPLAEIFQIVHETTRLPAENPALR
                     AIREGVVVGLANQTLLIARDGVERPIDDSAAPMHDESGAAIGAVLVFRDVSERKRAEE
                     AQARLAAIVESSDDAIISKSLDGIIRTWNTGAQRLFGHTPGQAIGQPITIIIPPERLD
                     EEKAIIARLLRGERVESFETVRVARDGRLLDISLTVSPLRDSEGHIIGASKVARAIGQ
                     RKQAEAALRASEGRHRFLAELAVATQRLADADEIMAVSARMLAEHLEVDRCAYAEIED
                     EAIYVITGDYTRGVASIVGRWPVVAFGEVHLRTMLANEPYVIDDVDTDPRAGSELAAY
                     RATNIQAVICVPLHKEGKFTAAMAVHQKTARHWTPDEVTLVHTVVGRCWEALERTRVA
                     RTLRESEARYRAIVEATPECVKVVDLDGTLLQMNPAGLKMLETDAATSLGQCVYDVIA
                     PEHREAFRAFNERVCRGHGGTLEFEIVGLRNTRRHMETTAVPLPAPGGGFVHLGVGRD
                     ITERAAAARALADSRARLDYAVRLSGIGFWHCDLPFDELLWDARVKEHFWLPPDARVT
                     IDTFYERIHPDDRAPTRAAIDRSIAGRVPYDIDYRTVNPTTGAIKWVRALGGTDYAPD
                     GAPQRFDGVTVDVTARKLDEQRLARALEREREQGRLLRQVADASLAIHSAGSVESVLR
                     VVAEEARRNLGAGLAFSSLGTGDDHVQTIIAAATADEHADPTQLRPPAALQPLATAAC
                     RTNRPMRRTHAELASHPRTPDEAIPRGWIAAPFVGPDGENLGLIQLCDKHHGEFGESD
                     EVVLVQLAHITSVAIENTRLYAALRDQDRRKDEFIALLAHELRNPLAPIRYGLQVMRL
                     SEDRAVRMRSQDIMDRQLAHMVRLIDDLLDISRISRNKTELRRSRVTLADAVNSAVET
                     ARPLIDAAGHTLEVVLPDQPVVLDADLTRLAQVFSNLLTNSAKYTEPGGKISLIAERH
                     ARSVSIAVRDTGIGIPAAALPTIFDMFSQVDRSIERSRGGLGIGLALVKGLVELHGGT
                     VTAESPGPGRGSTFTIRLPVDAPPAQGPADTTSGETTTTRPRRKILVVDDNRDSASTM
                     EIMLELLGDEVRTAHDGLDAITVAEAFRPELILMDVGMPRLNGYDATRRIRERPWGEQ
                     ITIVALTGWGQEADRARSKAAGCDGHLVKPVSLPDLEQLLADLTRPERR"
     gene            complement(17336..>17856)
                     /locus_tag="VGB85_11325"
     CDS             complement(17336..>17856)
                     /locus_tag="VGB85_11325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0134668.1"
                     /translation="CERFDVPALVDEVASTVGTLVRRGGNQLIVDCPPEVGSAVADRT
                     KLGQALLNLLGNAAKFTRHGTIHVVARREVEAGTPWLGIAARSWLSFAVLDTGIGIPN
                     ERMGELFLPFSQVGDAGQRQGGTGLGLTISREYCRMMGGDVSVTSEVGRGSCFTIRVP
                     ADVPAAIAQSTG"
BASE COUNT         2789 a         7098 c         5318 g         2651 t
ORIGIN      
        1 gctcgccgcc gctggtgctc gccctcgacg acctccactg cgccgacccg gccgccctcc
       61 acctcatcga gctgatcctc agcgaccccg ccgggggcca cctcctcgtg ctcgccagct
      121 accgcgacgc cgagctcggc gacgcccacc cgctgcggct cacccaggac cgcctgcgcc
      181 gcctcggcgt gcccgtctac gcgctcccgc tgcacccgct cgccgacgac gaggtcacgc
      241 agctggtcgc cgacgccctc ggctgcgagg tcgacgaggc cgccccgctc gccgcccgcc
      301 tcgacgccca cgcccacggc aacgcggcca tgctgcgcca cctgctgctc ggcctgcgcc
      361 gcgagggcca gctgaccttc gacagcgcct cgggtcgctg gcagtggaac acgtcgaccg
      421 cgatcggcag cggcaacccg ggcaacgcga gcagcccggg taacgtcgcc gccttcgtcg
      481 ccgcccgcct gcgacggctg ccgagcggcg tgcagcgggt gctggcgatc gccgccctcg
      541 ccggccgcgc gatcgacctc ccgctgctga gcgccgtcga ccgccgcggc ccgctcgcca
      601 ccaccgatgc cctgcgcgac gcattgcgag agggcttgct cgtgccctcc gacagcgccg
      661 tgcactcgct gcaagagaac cccggcacct cgcccggcac cccgcgcgtc acctatcagc
      721 cgctgcacga cggcatcgcg ctggccgcgg ccgacctgct gtccgccgac gagcgcgccg
      781 agatccacct gcaactcggg cgcaatttac tgcgccagca gagccccgac gacgacgacc
      841 tgttcgcgct cgtcgatcac ctcgaccgcg gcgccgcgtg catgaccgag ctcgccgagc
      901 gcgagcgcct cgcccggctg cacctcgagg cctgccgtcg cgcccgcatc gccaccgcct
      961 acgcggccgc cgtccgccac gcccgcgccg gcctcgtcac cctcgcggcg atcgtcgccg
     1021 agcccgacga gcacgtgatg ctacgcgagg ccctgccctc cgcctccgac ctgaagatcg
     1081 tccccgacgc gctgtggcag cgctatcccg accacacctt caagctgatg cgcgagtgga
     1141 tgcacgccga ggccctgcgc ggcgacctcg acgccgccga cgccctgttc gacccgctcg
     1201 tgcgccgctc gacccacgag ctcgcgcgcg ccgacctcta ccgcctcaag gcccagctcg
     1261 acacctacca cggccgcccg cacgacgcga tggcggccgg cctcgccggc ctctacggcc
     1321 tcggcgtcga gctgctcccg cggcccgagc ccgccgagat cgaagccgag cacctcgcgc
     1381 tgaccgccct gctcgccgac gtcgaggtca taaaattgat cgccggcccc gtatgcaccg
     1441 accccagggt ctccgccacc gccgagctgc tgtccgcgac cgggcccgcg gcgatgttcg
     1501 ccgacacgcg cctcgcctat cacgtcttta tccgcctcgt gcgcctgttc atcgagcatg
     1561 gaccgacacg cgacaccgcc tacggcctcg ccggctacgg cctgtacctc gccggtgcgc
     1621 ggcgtgacac cgcccgcgcc ttcgagttcg gccagtacgc cctcaagatg cgcgaccgct
     1681 tcgccgaccc gagcctcggc gctcgcatct ttcacatcgt cggcggcctg atcaccccct
     1741 ggacccagcc cttcgccgtc gccgccgcga tcctcgagca gggctacgcg atcggcgtgc
     1801 agaccggcga cttcgcggcc gcgacctaca acaccaccac cctcgtgctc gtgctcgccg
     1861 cccgccgcca cagcctccac gccgtgcgcg cgaccgccga gcagctgctc gtcgaggccc
     1921 gcccgatgct cgaggtctac ggctcgagca tcattcgctg ctcgatccgc atgtcgcgct
     1981 gcctcgctgg cgagctcacc gcgccgacgc ccggcaacgg cccgcgctgg ctcgccgagg
     2041 tgttcctccg cgacttcgag cgcgacggct caccgctgac cctgctctac ctccacatct
     2101 tcgccatctc ggtgctctac cacttcggcc tgcacgaccc cgccgacctc ccgcgcctgc
     2161 ccccctccgc cgccgagcgc ctcggcgtgc tcgtcacgcc gatggccgcg gattatttct
     2221 tttacaacgt cctgcacgcc tgcgccgcgc ccacgcccgc gacgatccac cccaagatcg
     2281 aggccgacat cgccgagctg cgcaagctcg cgtcgagctg ccccgccaac ttcgaggccc
     2341 gcgcccacct cgcgaccgcc gagtacgagc gcgtccgcgg caacgacgac gcggcagagt
     2401 ccgccttcaa gctagcgatc cgctcggccc gcaagcaagg cgaggccggc accgaggccc
     2461 tcgcctgcga gctcgccggc cgccacgccg ccagcatcgg cgacgacatc gtcggcacca
     2521 tgtacctgcg cgccgccgcc gaggcctacc agcgctgggg cgcccccgcg atcgcccgcc
     2581 gcctcgccca cgccgtcgaa gccaggcccg actgacgcgc catccccagc gtcaccagcg
     2641 ctgtacgagt agttgcactg ccaaggactc agccggacgg acgtgtccgg cgcgttgtca
     2701 acttgcatcg ccatggctat cctgcggctc cgagacatgc cgtctccctc ccacctcacg
     2761 gacgagaagc acgtcctgca tacgttgctc gccgcgctgt tcgacgctga cgagctgcgc
     2821 gtctttctcc acggctttcg tgctcccgaa gacctcggtc ggtcgctccc cggcgggatc
     2881 gccagtcatg accagctgat cggcgctgcc gtgcaggcgt tgcagcgtcg tggtctgttg
     2941 gatcaagaat tcttcggcgc gctggtgcgt gcccgcccgc gacgagcccg cgagatctgc
     3001 acggcagccc gtacgctcgg cgtcgagctg cccgacgcgg tcatggctgc cgcacaaaca
     3061 ggcgacgagc tggacgcact gctccgcgac caggcagaca gccacttcgg cgaacgcctg
     3121 ctctcgatga tcgggcacct cccccgtacg caccctctcg tccaccgcgc ccacctgatg
     3181 ctcgccgagc tccgacagca acgacgcgac tcggcggacc tcgatgtgca cgaacgcgtg
     3241 gccgcgtccg tattcgacct cgccctcgag gtggcgtcga cccgcacgcc aagcccgaga
     3301 caaaccgagg acgacgacag cggaaccagc gcgcagatgc tccacgccgc cgtgatcgcg
     3361 gcggtccagg aaaacgacgg cgagagggcc gtcaagcgcc tcctcgacct gacacgcctc
     3421 gccggccagc gcagcgcgat ggcaaatcgc gcgaccttgc tcgccgcgac catccacgac
     3481 ctccggcggc gatttcgtta catctcgatg ccgcaggagg ttcgcaacga gtggttgaat
     3541 accctcagcc agatcctgga gctcgcagat gatacgctcg cctcgtccgc cgacgcggac
     3601 cgttcatcgg tcgcgccgaa gccgttcgtg gagacggaca gcggcagcct gctccatgcc
     3661 cgccaggctt tctccctcgc ccgccaaggc gcccgcgatg cagcactcgc cccgatcacc
     3721 gtgcacctcg gcgggctcag caaggtccac ggaggtcggc acaagccatt cgtgctgcgg
     3781 gacgtctcgc tggacctccg ccctggcgag atcacaggcg tcgtgggccc caacgggagc
     3841 ggtaaaacca cgctcctgcg gatcatcgcc ggcgagacct cgcatgacag cggcaccctg
     3901 gcatatcccc gcctcgaccc ccgcgcccga gcaggccgtc cgaattggtc gcaagtccgt
     3961 cccttgatcg gatacgtggc gcagcgcccg ccacgctggc caggacggct cgccgagaat
     4021 ctgcaccgct gggccgccct ccacggcatc tacggcgacg ccaaccgcga agaggtcgag
     4081 ttctatctcc atcgtctaga cctcgaacgc ttccgcgacg cgacctggac ggagatatca
     4141 ggcggctacc agatgcgctt cgagctggcc cgcgtgctat gtgccgcccc ccagctgctc
     4201 gttctcgatg aacccctcgc ccccctggac ctcgctaccc aggatctcta cctccaggac
     4261 ctccgcgaca tcgcatccgc cccgtccagg ccgatcgccg tgatcgtgag ctcccagcac
     4321 atctacgaga tcgagtcgat cgcagaccac atgctcagcc tgggtgaaga tggttcggtc
     4381 cagtacagcg gccccgcgcg cgctatcctg catcccgagg acgaccgcat cttcgagata
     4441 gaggccgacc tccatcctca ccacctcgat gccttgcgcg gtgccaaatt agtacgcgat
     4501 agccgtcgga gaggtcggcg cctactccta agcgcatcag caaccgtatc cggcgccgac
     4561 atcctcgatt ggctccgcca tcacggtata cccctcgtct tattccaaga catcagtgac
     4621 tcctgcgcca gattggcaaa atggaacttc gcagattgac ataccgctct ctttgtcacc
     4681 aatggtcaac cctggaccaa tatctgctct accggcaccc tatcgcatgg tcgctgcgct
     4741 tccattacgt ggtcgcaatt ttcgccccgg ccctcatcct ttccgttctg tacgcatgta
     4801 tgttacctct gaccctctta agttcgccgg atgtagaggc gatcttcgtt tggtgggcca
     4861 ccctctccgc cacctcagcg gcagtatggt ttctgaagtg ccagagttcc ctgaagtgta
     4921 caaccgctcc gcttcgcggg gggtggtttg ccgcgcgact aatgtgtgtc gggctcatcg
     4981 ctctgcccgg attcgcaacg gcagacattt tgatctttcg cctccagaat acaacggtta
     5041 gcacatcggc aaccgccgtc aaaaatggat attccgcact ctgcggctgc acccagcaac
     5101 agatcaatga cagaagcccc gtccctgatt tcaagagatt ttacagcatc agcggagaaa
     5161 gcctattcat cgaatctccc cgatgcccaa agatcccgct atcggttgcc tcagccgaat
     5221 gctggaatga catagtttct tcagagacgt cgtccgacgc cttcatagaa cactataacc
     5281 tgatgcaatt ttgccaaaat ccttggtggc cacattacag ggagagctgc accttggaag
     5341 ttctacagga aggaatacat aacgtacttg gtcaaaaatg cattgatgag gattgccttg
     5401 catctgccga aaggattggc cgtttccaaa ctgggccgtg gacggcgact tcatttttgc
     5461 tacccggacg acgtgagcat ctctacggtc ttttgatctg tctactgctg acggttctcg
     5521 ggagcgccct taagaatgtc cactggagag caattacagc cctctctctg atactattct
     5581 tcctctggct cctactccct ttaggagcat tccttggaaa tctgcctgcc aacaatagaa
     5641 catactccgg caccgcactt ttaatatttt ctctagctac atttaccgta ttgcacactg
     5701 tgtttttccg caagcggcgt ggcgccttct caattgacat aagccttgca gccagctaca
     5761 tcactctccc catactcccc gcattgatga ttggcacgct tatccgtcca gagggcacga
     5821 gtgatgaaga catactgagc atcatcagca atgtaatcgt gatggacgtg gattttgccg
     5881 agttatcggc ctttacttgg gtgatgctga tatcacctgc ttggacgctc gcgtttgcag
     5941 cgtttttgga gcccttggct cgtcgatggg cccatctccc gcgttaatcg actactacta
     6001 gttgaatcat gcttgatctc tgagtgcggg cccggtcggc ttcgccgccc ccgcgtccaa
     6061 ctctatctcg agttgctacc tcatggtccg cacgcgattg taccatggta gcgtcggcgc
     6121 tagcgccgtg accgggctcg cccgacggac gctaagcggc taccctctcg ccgatgatga
     6181 tcgccggcta caaccacgac gagctcctgc aagaagtcct cgtgcgcccg cgcgaggccc
     6241 acgtccctta cgccgacctg cgtgaggtcg tgcggcccgg gtggcatcag atcgtcaccc
     6301 cgtcgttttt tggcggcggc ctcaacggcg tcgacctcgc cgtgctcgac catcttgacc
     6361 atgccaccgc cgacgccgtc atcgacgcga cgatcgccga gtacgacgcg ctcggcatcc
     6421 gctttcgctg ggagatcggg cccgactcgc gcccgcgcga cctcgccgcg cgcctcgagg
     6481 cccgcggtct gacctcgaag cgcgtgctgc tgctcgccgc cgcgatcgcc gacctcgaca
     6541 tcgaggcacc ccccgacgtc gtcgtcgagc cggtcggcga gcacaacctc gccagcttcg
     6601 tctcggtcgt cgccgccggc tggggcatgg tccccgaccc tctgatcgcc tacgagcgag
     6661 ccgtggtcgc gcaccgcagc acgaactcga ccacgctcca ccagagcttc ctcgccacga
     6721 tcgacggcga gccggccggc gccgccaatg cggtctgctt cccgcgctcg ctctatctga
     6781 tgggggccgt cgtcctgccc gcgcaccgcg gccgcggggt ctaccgctcg ctgatcgcgg
     6841 cgcgcttgca cctggcgcgg gcccgcggcg tcggcctcgc cacttcccag gccctcgccg
     6901 acacctccgc accgatcctc gcgcgcatgg gcttcgtcac cgtcgccgaa cagctgtcgt
     6961 tcgcgcgctg agcgggtgcg gcccccctca ccctcgcccg ctcacgcccc ctcgcccttg
     7021 cccccgcagc gcccgcgcta tgctgccagg atgcgacgcg ccctcgtccc cctgctgagc
     7081 gccgcggccc tgcactgcgg cgacgccacc tcgaccagcg acagcgccgg cgcgaccacc
     7141 accgcgacca ccaccgcgac caccacgacc accgccccca ccgagccgac cccgaccacc
     7201 ggcggcaccg cctccgccag cgccccgacc acttcagaaa ccacctcggg cgcgagcgac
     7261 agcgccagcg acgacagcgg gcccccggcc gacgagtgtg ccaccagccc gcactaccgc
     7321 gtcggtgccg gcatcgccga cgtcaccggc ccctccgccg agctggtgat gatgggctac
     7381 tcgcagccga cgcagaccgc cgagggcatc tcgacgcgcc tgtggtcacg cgccttcgtc
     7441 gtcgagagcc cctgctccgg cggcaggatc gtgttcgtca gcgccgacct cggccaggtg
     7501 ttccagtccg tgcacctcga ggtcctcgag cgcctcgagg cccgcttcgg ccccggtcta
     7561 tacacccgcg acaacgtcgc gctgtcggcc acgcacaccc acagcggcct ggccggcttc
     7621 tcgcattaca ccctctacaa cctcgccgcc ctcggcttcg accccgacaa cttcgaggcc
     7681 gtcgtcgtcg gcatcgtcga gtcgatcgcc cgcgcgcacg acaacctcgg cgagggcgtc
     7741 gtcaagatcg cccagggccc gctgcaggac gccacctgga accgttcgcc gcaggcctac
     7801 gccaacaacc ccgaccccga gcgcgccgct tacgaggccg agcacgcgcg cgacgtcaac
     7861 acccgcatga ccgtgctccg cctcgagcgc accgccgcgg gcgcgaccga cggcgacccg
     7921 atcgccgcga tcgcgtggtt ctcgagccac gcgacctcgg tcagcaacgc caaccacctc
     7981 gtcagcagcg accacaaggg cctcgcctcc ctcgacttcg agcgctcgat gggcgccgac
     8041 tacatgagcc ccgcgccatt tgtcgccgcc ttcgcgcaga gcgacgccgg cgacgtcacg
     8101 cccaacgagg tgatcgtcgg cgagcccaag aacacggtca agcgcccgtg cgacggcagc
     8161 cccgaccccg agtgcaccga cctcgccaac gctgcgatcc acgccgacat ccaatacgcg
     8221 cgcgcgcgcc agctgttcga cgaggcgacg gagctcctgc gcggcccggt cgacggccgc
     8281 ctcgccttct tcgacatgga gaacgtcacc gtcgccgacg agttcaccgg cgccggcgag
     8341 aagcacacct gctccgctgc gatcggcgcc tcgctggctg cgggcgccga ggacggcgtc
     8401 ggcgtcgagt tcatccagga gggcatctcg ctcggcacga tcccgctgtt tagcctcgtg
     8461 cccgaggacc aggtctgcca cgccgaaaag acgatcctgc tgccgaccgg ccgcatgcag
     8521 ccccacccgt ggaccccgcc gatcgtgccc gcgatggtcc tgcgcctcgg caacctcgcc
     8581 atcattccca cccccttcga ggtcaccacg atctccggcg accgcctgcg cgcgaccgtg
     8641 ctcgaccgcc tcgccggcac cggcgtcgac cgggccgtca tcgccggcct cagcaacggc
     8701 tacgccagct acgtcaccac ccgcgaggag tacaaggccc agcactacga gggcgcctcg
     8761 accctgttcg gaccgtggac cctcgccgcc tggcagcaga acttcgccgg cgtcgccgag
     8821 gccctgcgcg acggcaccga cgccgtgcac accggcgtgc ccgtcgacct ctcggacgcg
     8881 cagatggtcg agaccctgcc cgtcgtcgcc gactcgccct gcgccttccc ggacccggcc
     8941 ctgtgcatcg gcgagaagtt cggcgacgtc gtccaggccc cgcccgcgac cgtcgaggcg
     9001 ggcgcgcacg tggtggtgcg ttttcgcggc gcgcacccgc gcaacgacct ccgcactggc
     9061 accggctacc tggagatcca gcgcaagctc ggcgaccagt ggcagacgat cgcgcgcgac
     9121 tgggaccccg agaccacgtt ccgctggcag cgcaccggcg gcggactcag cccgacctcc
     9181 gaggtcacga tcgactggcg ggtccccctg gacgccgtcc ccggcgagca ccgcgtcgtc
     9241 tatcacggcg acgccaggac cctgctcggc gtcatcaccc cgatctcggg cacgtctccg
     9301 agcttcaccg tgcaatgacg agctcgttcc ctcgcccccg ctcccctgtt agcgtcggcg
     9361 catgcgcctc cccggcctgg ccctgctcct cccgctcgcg gcctgcttca gccccaaccc
     9421 cgcgttcgtc ccgaccaccg atggcaccgg cggcacgagc accggcacca ccgccaccac
     9481 cgccgtcgac agcaccgaca gcgatccaac gaccaccacc accactggcc cggacacgac
     9541 cgggagcgtg acgatcgagc cgctgacctc gaccagcgag ccgctgacct cgaccagcga
     9601 gccctcgacc agcgagccct cgaccggcga gccgctgacc agcagctcga gcagcaccgg
     9661 ccagcccgac cccccgaact gcgagctcga caaggccgac gacgatcccg ttcgtgtcgt
     9721 ccgcgacctc gccaacaacg ccaaggagat cacgcagtgt gacgaggaca cccccacgac
     9781 ctggaagggc gcgctgaccg tccaggacgg caagctgacg atgtacctca atgaccagtg
     9841 caacgggggc ggcccgggcc tgctcgtcgg cgaggcctgg ccggcgtcca acaactggac
     9901 ctatccctgc gtgcagacgc gggtctactg gaaaaaggtc caggggcagt gccggatcag
     9961 cgccctgcgg gtggtggagc tcaaggagcc catgatggac cccgccccgc cgatcatcct
    10021 cgcctcgctg tcgcacccga gtgaaccggc ggagttccag atgtggcccg cgctgatcgc
    10081 gtcggggatg ccgtgcggct gcccgccaga cctgcccgac tgctgcgacc ccgaccccgg
    10141 gcagtacgag ctcgacttcg acgacaacgt cgtcatcgac tcgaccctcg agaagacctt
    10201 ccctcgcttc gggtccgtct accgcctcaa gaacatccag tcgtacatcg gcgctggctg
    10261 ccagaacgac cccggctccg acgtccacat cgactggttc ctcgagcgcc tgccgccgta
    10321 gtcagtcgat cgggtgcatc gcgccgcgga tcgtctcgtg cacgaccccg ggcccgacct
    10381 cgagccggcg cgcgatgtcc tccatcacgt gcagctcgtc gtcgctgacg tgcccgtccg
    10441 ccgccgcgac gatcgtcagg tgctggacca gctgcgcgcg atacagcagc ggtacctcga
    10501 gcgcgtcgcg gatcctgccg tcgaggtcgc gcagcacctt gtcgacgtcc gtcggcgcgt
    10561 ccgcgtggtc cgaacccagc agccgcccga tcgcgtcgag ctcgctcgcc tcgaccccgc
    10621 ccgaggccgc cgccaccgcc acccccgcgg tgtacaaaag ccgccgcagc gtgtccgagc
    10681 tctggctctt ctcctgcaga tacgtcggct ccatcagcga catgtcgcgg tcgaccatcg
    10741 tctcgacctc gtcgagcgtc cacgcgtcca ggctcgactt gcccatcgcg tcgaggaacg
    10801 gcttgctgcg gctaaacgcc cacaccgcgc gcacgcgcac cgggttgtac gggtgcgtgc
    10861 tgaagcagtc gagcgtgtcc tcgtcgtact ccttctcgcg cgcctccggg gccgccgcca
    10921 ggctctcgac ctggcgggca aaggtctcca ggtccggcac cacccgcggg ctcgacatcc
    10981 cgctggcgag cttgaaaaag ccgctggcgg ccgcctgcgg gtcctgggcg cacagcaggc
    11041 cgatgcggtc cgccgacagc tccgcgctgc gacaccacgc ataaagcttg agcgacacgt
    11101 ggcgcgacac gaacgggatc ccgagctcct cgaccatcgc cgtgaccggc atcggcaggc
    11161 cgtagtggtc gaaggccgcg tggccgagct cgtgaccgat cacgaagcgc agctcggccg
    11221 gcgtgaacgc ctccaacaac cggctcgaca gcgtcagcac cgtcggcccg cggacctcgc
    11281 ggtaacacgt cgcgttgaac tggtgctcgg gggtgacata gacctcgacc ggcccctcgt
    11341 gaccgatcaa cgccttgcac tgcgcgaccg tgtcggcgat ctccggcgac atcgagcggg
    11401 tcagtcgcag cgtcgagctg agcagcccgc ggcgataacc gtagcccttg ccgccgcgga
    11461 cctcgcggat cttcgccagc gccttgcgca ccccgaagtc gccgacgacc tcgtcacgca
    11521 gctgttgctc gcgccgcacc tgaaaatgct tcggatcgat ctgcattcgc gctcacccct
    11581 cgccgtcccc aaacatctcg aacggcctgc accggggatc cgcggtgcag gcaccgtcgc
    11641 gcactacgag cgcgcgtact tcagcgtcaa gacctcgagg atcttggtcg ccagcgcgtc
    11701 ttcgacttgc agcacccgct gcatcgcgtc gagcatcgcg tcctcggcct cgtcgacgtc
    11761 gccgctcgcc atcgcgatgt cggccgcccg caggtagcac ttgttcttga ccgtctgggt
    11821 cgagaggtcg ccgagcgcct tcaccgagtc cttgaggttc gggaagcgct tgagcacctt
    11881 cgccgcctcc tgatacaccg tgccgaagtc cttgccctcg aactcgggca acgaattgaa
    11941 gtacgcctcg acggtcggga tctcctgctc gtcgaagctg ccgtcggcgc cgcacatcag
    12001 cagcatcgcg tgcaagagca gggagtcgtc cgccggcttc ttctgcgggg tgaccttacc
    12061 ggtgagtttg gagagtagtc ccatgacgtt gtgctgttcc tcgtgagatg tgcgagagca
    12121 ggggcccccg cgatccgcag cagtgtcgac cacacgcccg cgggtgacaa gcccaccgcc
    12181 cccacgcccg cttccgcgcc cctcccgcac cgcacccgcc atgccaatgg cgcccgcaaa
    12241 acaccgccca ccgccaagca ttgcccgcca ttgcccgccc attgcccggt gacgggcggc
    12301 cgccgatgat cgggcacact cacctgtccc cgaggacatg tccgcgaaca cccgcggcga
    12361 cggcaggtat cggcgccgat ccggcgccga ccctgtgcac cgcaccaccg ggccagagaa
    12421 ccggtgtttt ccggcacctc ggcggctgtc cgggatcgca taaactcggg tacgatcgcc
    12481 cgtccgcccg cggaccgtga gcccgctatg cccctcccga ccgagccgat cggcagcatc
    12541 ccccgacccc cggcgttgat cgccgccgtc gcggcgatgc gccccgggga gaccccggac
    12601 gctcacctgg acggcctcta cgacgccgcg gtgcgcgaca ccatcgcgcg cttcgaggcc
    12661 accggttcgc ccgtcgtcac cgatggcgag cagcgcaagt atcacaactt ctggacctac
    12721 tgcgtgcacg gcacgacgaa caccgccccc gacgggttca agatcccctt cgccgccggc
    12781 cacacgcggc ggatgccgcg gctgacctcg ggcccgttca agtacatgcg ccacgccgac
    12841 agctacctcg acctcgcgct gcgccacgcg ctgcgcccgg tcaagcaggc cgtcatctcg
    12901 ccctcggccc tgagcctgat gtacccgccc gaggagctcg ccggctaccc gcgcgcgcag
    12961 ttcatcgacg acctgctcga cgagcacgtg accgaggtcc gggcctgcct gcaaaagggc
    13021 gcgcacgcgg tccagatcga ctttaccgag ggtcgcttcg ccgtcaagct cgacccctcg
    13081 ggcgacctgc tgcaccgcta catcgatcta aacaacctcg ccctcgctcg cttctccgcg
    13141 gacgaccgcg gcaggctcgg cgtgcacacc tgccccgggg gcgaccgcga ctcgacccac
    13201 agcgccgacg tcgactacgc cgagctgctg cccgccctct tcgagctcga ggtcggcaac
    13261 ttctacgtcg ccctcgccgg cgagcccgac cgcgtccgcg tgttgcagct cctgcgcgag
    13321 cacctgcggc cgcaccagcg cgtgttcgtc ggcgtcgtcg ccccgatcga cccgcgcgtc
    13381 gagacccccg aggaggtccg tgaccgcgtg ctggaggccg cgcgcttcat ccccgtcgag
    13441 cagctcggca ccaccgacga ctgcggcttc gcgccgtttt gcgacgacac ctcgaccagc
    13501 cgtgacaccg ccttcgccaa gatccgggcc cgcgtgctcg gcacggcgct cgcatcgcgg
    13561 gcgctcgggg tcgactgatg gccgacgacg aggagaagca gctgcgctcg gtcgcgctgc
    13621 aaaacgccaa cgcgatcctc cacacgcgcg cgcgcgccga gcgtgagctc gccaaggagc
    13681 gcgagcgcct gcggatcacc ctcgccagca tcggcgacgc cgtcatcagc accgacgtcg
    13741 acggccgcgt caccttcctc aaccgcgtcg ccgagcacct gaccggctgg acgcaggccg
    13801 acgccgccgg ccgtccgctc gccgagatct tccagatcgt ccacgagacc acccgcctgc
    13861 cggccgagaa ccccgcgctg cgtgcgatcc gcgagggcgt cgtcgtcggc ctcgccaacc
    13921 aaaccttgct gatcgcccgc gacggcgtcg agcgcccgat cgacgacagc gccgcgccga
    13981 tgcacgacga gtccggcgcg gcgatcggcg cggtgctggt gttccgcgac gtctcggagc
    14041 gcaagcgcgc cgaggaggcg caggcgcggc tcgccgcgat cgtcgagtcc tccgatgacg
    14101 cgatcatcag caagagcctc gacggcatca tccgcacctg gaacaccggc gcgcagcgcc
    14161 tgttcggcca cacccccggc caggcgatcg gccagccgat cacgatcatc atcccgcccg
    14221 agcgcctcga cgaggagaag gcgatcatcg cccgcctgct ccgtggcgag cgcgtcgagt
    14281 cgttcgagac cgtccgcgtc gcccgcgacg gtcgcctcct cgacatctcg ctcaccgtct
    14341 cgccgctgcg cgacagcgag ggccacatca tcggcgcgtc caaggtcgcc cgcgcgatcg
    14401 gccagcgcaa gcaggccgag gccgccctgc gcgccagcga gggccgtcac cgcttcctcg
    14461 ccgagctcgc ggtcgccacc cagcgcctcg ccgacgccga cgagatcatg gcggtctccg
    14521 cccgcatgct cgccgagcac ctcgaggtcg accgctgcgc ctacgccgag atcgaagacg
    14581 aggcgatcta cgtgatcacc ggcgactaca cccgcggcgt cgccagcatc gtcggccgct
    14641 ggccggtcgt ggcgttcggc gaggtccacc tgcgcacgat gctcgccaac gagccctacg
    14701 tgatcgacga cgtcgacacc gacccccgcg ccggctccga gctcgcggct taccgcgcga
    14761 ccaacattca ggccgtcatc tgcgtccccc tgcacaagga gggcaagttc accgccgcga
    14821 tggccgtcca ccagaagacc gcgcgccact ggacgcccga cgaggtcacg ctcgtccaca
    14881 ccgtcgtcgg ccgctgctgg gaggcgctcg agcggacccg cgtcgcccgc acgctgcgcg
    14941 agagcgaggc ccgctaccgc gcgatcgtcg aggccactcc ggagtgcgtc aaggtcgtcg
    15001 acctcgacgg caccctgctg cagatgaacc ctgccggcct caagatgctc gagaccgacg
    15061 ccgcgacctc gctcggccag tgcgtctacg acgtcatcgc ccccgagcac cgcgaggcgt
    15121 ttcgcgcctt caacgagcga gtgtgccgcg gccacggcgg caccctcgag ttcgagatcg
    15181 tcggcctgcg taacacccgg cgccacatgg agaccaccgc cgtccccctg cccgcgccgg
    15241 gcggcggctt cgtgcacctc ggggtcggcc gcgacatcac cgagcgggcc gcggccgcgc
    15301 gcgccctcgc cgacagccgc gcgcgcctcg actacgccgt gcgcctgtcg ggcatcggct
    15361 tttggcactg cgacctgccc ttcgacgagc tgctgtggga cgcccgcgtc aaggagcact
    15421 tttggctccc cccggacgcc cgcgtcacga tcgacacctt ctacgagcgc atccaccccg
    15481 acgaccgcgc accgacgcga gcggcgatcg acaggtcgat cgccgggcgc gtcccctacg
    15541 acatcgacta tcgcacggtg aacccgacca ccggcgcgat caagtgggtg cgggctctcg
    15601 gcggcaccga ctacgcgccc gacggcgccc cgcagcgctt cgacggcgtc accgtcgacg
    15661 tcaccgcccg caagctcgac gagcagcggc tcgcccgcgc cctcgaacgc gagcgcgagc
    15721 agggccgact gctgcgccag gtcgccgacg cctcgctggc gatccactcc gccgggtcgg
    15781 tcgagagcgt cctgcgcgtc gtcgccgagg aggcccgccg caacctcggc gccggcctgg
    15841 cgttctccag cctcggcacc ggcgacgacc acgtccagac gatcatcgcc gccgcgaccg
    15901 ccgacgagca cgccgacccc acccagctgc gcccgccggc cgccctccag ccgctcgcga
    15961 ccgccgcgtg ccggaccaac cgcccgatgc ggcgcaccca cgccgagctc gccagccatc
    16021 cccggacccc cgacgaggcg atcccacgcg gctggatcgc ggctcccttc gtcggccccg
    16081 acggcgagaa cctcggcctc atccagctgt gcgacaagca ccacggcgag ttcggcgaga
    16141 gcgacgaggt cgtgctcgtc cagctcgcgc acatcacctc ggtcgcgatc gagaacaccc
    16201 gcctgtacgc cgcgctgcgc gaccaggacc gccgcaagga cgagttcatc gccttgctcg
    16261 cccacgagct gcgcaacccg ctggcgccga tccgctacgg cctgcaagtg atgcgtctgt
    16321 ccgaggaccg cgccgtccgc atgcgctcgc aggacatcat ggaccgccag ctcgcgcaca
    16381 tggtccgcct gatcgacgac ctgctcgaca tctcgcggat cagccgcaac aagaccgagc
    16441 tgcgccgctc gcgggtcacc ctcgccgacg cggtcaacag cgccgtcgag accgccaggc
    16501 cgctgatcga cgccgccggc cacaccctcg aggtcgtgct ccccgatcag cccgtcgtgc
    16561 tcgacgccga cctgacgcgc ctcgcccagg tgttcagcaa cctgctgacc aacagcgcca
    16621 agtacaccga gcccggcggc aagatctcgc tgatcgcgga gcggcacgcc cggtccgtct
    16681 cgatcgcggt gcgcgacacc gggatcggga tcccggccgc cgcgctgccg acgatcttcg
    16741 acatgttctc gcaggtcgac cgctcgatcg aacgctcgcg cggcggcctc ggcatcggcc
    16801 tcgcgctcgt caagggcctc gtcgagctgc acggcggcac cgtcaccgcc gagagccccg
    16861 gacccggccg cggcagcacc ttcacgatcc gcctccccgt cgacgccccg cccgcccagg
    16921 gccccgccga cacgacatcc ggcgagacca cgaccacccg accgcgccgc aagatcctcg
    16981 tcgtcgacga caaccgcgac tccgccagca cgatggagat catgctcgag ctgctcggcg
    17041 acgaggtccg caccgcccac gacggcctcg acgcgatcac cgtcgccgag gcctttcgcc
    17101 ccgagctgat cctgatggac gtcggcatgc cccgcctcaa cggctacgac gccacccgca
    17161 ggatccgcga acgaccctgg ggcgagcaga tcacgatcgt cgccctcacc ggctggggtc
    17221 aggaggccga ccgcgcccgc tcgaaggccg ccggctgcga cggccacctc gtcaaacccg
    17281 tcagcctgcc cgacctcgag cagctgctcg ccgacctcac ccgccctgag cgccgctaac
    17341 cagtgctctg ggcgatcgcc gccggcacat ccgccggcac gcggatcgtg aagcacgagc
    17401 cccgtccgac ctcgctggtc acgctgacgt ccccgcccat catgcggcag tactcgcgcg
    17461 agatcgtcag cccgagcccg gtgccgccct ggcgctgacc cgcgtcgccg acctgcgaga
    17521 acggcaggaa cagctcgccc atgcgctcgt tggggatgcc gatgccggtg tcgagcaccg
    17581 cgaagctcag ccagctgcgc gcggcgatcc ccagccaggg ggtgcccgcc tcgacctcgc
    17641 ggcgcgcgac cacgtggatc gtcccgtggc gcgtgaactt ggccgcgttg ccgagcaggt
    17701 tgagcagcgc ctgcccgagc ttggtgcggt cggcgaccgc ggagccgacc tcgggcgggc
    17761 agtcgacgat cagctggttg ccgccgcggc gcaccagggt cccgaccgtg ctcgccacct
    17821 cgtcgaccag cgccggcacg tcgaagcgct cgcact
//
LOCUS       DASWNJ010000677         7546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_682630, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000677 DASWNJ010000000
VERSION     DASWNJ010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7546
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682630"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VGB85_11330"
     CDS             complement(<1..542)
                     /locus_tag="VGB85_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134669.1"
                     /translation="MTVVVVQRDRRAFGLGLLGGLLGWASAATVACDVRGSKDPGRAA
                     DCPACVCKCETGDVAAAESKPPGPGLDAQELIESATRKMNHDDGTGCISDLDAYDRLA
                     PRRKSSDPQNTLAITRAQCMMLAGQCDSGKGLLRGAYENTAAAKWGPEQLERSVEALA
                     SMHCRGKMSPRDELLQGMMSL"
     gene            complement(644..2158)
                     /locus_tag="VGB85_11335"
     CDS             complement(644..2158)
                     /locus_tag="VGB85_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134670.1"
                     /translation="MQLRAVLPLSLLSLSLGLASCGPKIPRTTATRESGQSGLMNESF
                     AAQNACNPKNHLRPFIIEWDATDKSSFEAYAASDIVVVRYEGCDMTVLEECRNDSIRG
                     SQGAYKPVEWTSGALEKLDIVNQGELHAKLPLSSATLGGRIAGGEKFHMEYYVSGTRN
                     ATRDAVYREDIQSNPGCEGATHFVYGYNLGAFALGSLNELNASAEASIYGFGAGGSTT
                     KSRKADKQGGNLGTCKSDTATEVEGCKAPIRLTLRPIRDGANPQKEAMKAEDTDAALN
                     AAGAVNMKVEMSDMARSHYETAMTKIAARDGKGCLSELDAYDRLEPTRKTTDGKVQMS
                     MFRAQCLMLAGKCDPGKLLLRKNYENGPMASYGPDHIDKAVEGTASQYCQGKMSPRDE
                     LLKGLLALNMASVKKMTVAECTEAYEAVRRNKDKVKPKDEDDHQINSLNETFYYAATN
                     CFARAGDCKQARVVFDAHMPPERYAHVTDPKTKAETLTNVFETTASKCKSKPAQ"
     gene            complement(2309..3811)
                     /locus_tag="VGB85_11340"
     CDS             complement(2309..3811)
                     /locus_tag="VGB85_11340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HEY0134671.1"
                     /translation="MARLHTRLTSLLAPLLVPVTAALLFAPEASAGSEPRTLFGKPVN
                     DPPAMTPEEIEARAAGFRELLERQGQVLKGNQVLPKSALEPTLTQPSDPVEAWDEPVH
                     RATTFLNFFGGPLTSGGNASKGESPCVQGKVDYPGFGGSEQTALAIIQVFKDAAKPFG
                     LRIVFDEPPPKHLPYSQVMMGGSPQIIGLPNGVLGVACNLDCGDAWWRDTTFAFTEET
                     NNVGILGTTALQEAAHAWGLDHIDGENNIMYPYATPGNKVWADTCTVYNDATGGIGCQ
                     YVHKQFCPEGAQNDVAELMAYFGPDSPDTVPPTVTMFSPTDGQQYKAGDTVHIEVEVG
                     DDYEGYGWRLMVPELDQEQPVYNGQKVWDLPAPPKGVYTIRVEAVDHDRNIGFAEAKI
                     FVDTVPGEENTTGDTPTTGDSDDGSGSDTSEPTGGESSGDGGEASSGNGASSDADTAQ
                     DSDDSGMETGDDKGCSCVTPASSSSSPSSWAWLLLFGGLGLRRRRSRMVG"
     gene            complement(3933..4439)
                     /locus_tag="VGB85_11345"
     CDS             complement(3933..4439)
                     /locus_tag="VGB85_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134672.1"
                     /translation="MRSTLIALAFMTGGCLVEYVLPEGDSSGGDTDDSMGHVVTGDPA
                     PTSTAGTTGDIQTTGDPCDEGLTRCGDACVDLSRDDAHCGSCDEYCKSDERCIASECR
                     DILPVDCAACPCDACPEASGIVESTTSGGGSDAEKHLCCPPPDGAEAVLCVVGDAEDP
                     LVCPTGGD"
     gene            4668..6494
                     /locus_tag="VGB85_11350"
     CDS             4668..6494
                     /locus_tag="VGB85_11350"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEY0134673.1"
                     /translation="MVEAPDKLRRTGGLGGLGNLGSLACSMGLLLATGCYTGADLDGD
                     DDTVTGTASNPSGADAGEDSTPTTGATAGTAGTAGTADTGDTADTGDTAGDADGLPQT
                     SRYPRLSHTQWENSVQDLFFLPEPTGFSELFIGDPISGGFDNNSEALKVTATLWTDYQ
                     RGAEQVADLVAGNPEILAKIAPPTEGDSDAQARAFIIAFGKRAYRRPLTPEEVERHLQ
                     IFQSGVSKPGDLEPFAAGVRLVLQAMLQSPFFVYRVEASDVDDGSGKIKLGDYELASK
                     LSYMLWNTMPDDELFAAADAKQLQDATGVAAQATRMLEDPRARTMVATFHHQLLHVDH
                     YSDVYKDPTKFPNYDPAMNAMMATETMMFVEDVVFGQNGDLATLLTAPYSFVNDKLAA
                     LYGVEAPAGSEFAKVDLDPAQRAGILTQIGFLSANAGAFENDIIHRGVFMNLQLICSP
                     LPPPPNMVPPLPPPVEGETLRERVDRHTGEGTCGAGCHGGMINPLGFAFENYGPMGQW
                     QTMDAGQPVDAAAAFQFKDGLKPYDGAVELAQLMAESPDAHRCYVAHLLEYGYARGPQ
                     KADDALIQKVAEASQAGTLSIKQMILELTQSEAFLYRAPVEG"
     gene            6497..>7546
                     /locus_tag="VGB85_11355"
     CDS             6497..>7546
                     /locus_tag="VGB85_11355"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEY0134674.1"
                     /translation="MPRKNNFSRRAFLYGAGGVTVALPFLDALRPRKVVAAGPDRPRY
                     AVFVRQANGCAQAEGMEPEQFWPHELGPVTKESLSTTDADRAVGELAEYADKLLMVRG
                     TRFPFPGNGCGHSGGGNQVLTAQKVSIDPMGNKSLAMGESVDNRIARELNPLSNPEPL
                     AVYAGAMGGYINEVLSYRGEKDLRAAERNPWNVYQKMVGITMLPEQQIMDIKARRTSV
                     NDLLKEQMTALMSSPKLSSDDHDRLQLHFDAIRDIEIELACSLPPQEELDAMAAQMDY
                     SGQDDRMQIIVQMHCNLIALAFACDYTRAATLQIGDGNDGTEYLIDGNKFPRYHQISH
                     RIYSDGAEGDPIPDAL"
BASE COUNT         1138 a         2691 c         2518 g         1199 t
ORIGIN      
        1 agcgacatca tcccctgcaa cagctcgtcg cgcgggctca tcttgccccg gcagtgcatg
       61 ctggcgagcg cctcgaccga gcgctcgagc tgctccggcc cccacttcgc cgccgccgtg
      121 ttctcatagg cgccacgcag cagccccttg ccgctgtcac actggcccgc gagcatcatg
      181 cactgcgccc gcgtgatcgc cagcgtgttc tgcgggtcgc tcgacttgcg ccgcggcgcg
      241 agccggtcgt acgcgtcgag gtccgagatg cagccggtcc cgtcgtcgtg gttcatcttg
      301 cgcgtcgccg actcgatcaa ctcctgcgcg tcgagtcccg ggccgggcgg cttcgattca
      361 gctgccgcga cgtccccggt ctcgcactta cacacgcacg ccgggcagtc cgcggcgcgc
      421 ccggggtcct tgctcccgcg cacgtcgcag gcgaccgtcg ccgcgctggc ccaaccgagc
      481 agcccaccga gcagcccgag gccgaacgct cgccgatctc gctgaaccac cacgaccgtc
      541 atcccgccag tgtaccggtt ttcactcgcg cttcgaggct gtcggccgcg aagcgcggga
      601 gcatccgccg cgcgcgtacg agcgcggcgg cgtcacgacc tcatcattgc gccggcttgc
      661 tcttgcactt cgaggcggtg gtctcgaaca cgttggtcag ggtctcggcc ttggtctttg
      721 gatccgtgac gtgcgcatag cgctcgggcg gcatatgggc gtcgaacacg acccgcgcct
      781 gcttgcagtc gcccgcccgg gcgaagcagt tggtggcggc gtaataaaac gtctcgttga
      841 gcgagttgat ctggtggtcg tcctcgtcct tgggcttgac cttgtccttg ttcctgcgga
      901 cggcctcgta cgcctcggtg cactcggcca ccgtcatctt cttgacgctc gccatgttga
      961 gcgcgaggag gcccttgagc agctcgtcgc gcgggctcat cttgccctgg caatactggc
     1021 tcgcggtccc ctcgacggcc ttgtcgatgt ggtccggccc gtagctcgcc atcgggccgt
     1081 tctcgtagtt cttgcgcagc agcagcttgc cggggtcgca cttgcccgcg agcatcaggc
     1141 actgcgcgcg gaacatgctc atctgcacct tgccgtcggt ggtcttgcgc gtcggctcga
     1201 gccggtcgta cgcgtcgagc tcgctgaggc agcccttgcc gtcgcgcgcg gcgatcttgg
     1261 tcatcgccgt ctcgtagtgt gagcgcgcca tgtcgctcat ctccaccttc atgttgaccg
     1321 cgccggcggc gttgagcgcc gcgtcggtgt cctcggcctt catcgcctcc ttctgcgggt
     1381 tcgcgccgtc gcggatcggc cgcagggtca ggcggatcgg ggccttgcag ccctcgacct
     1441 cggtcgcggt gtccgacttg caggtgccga ggttgccgcc ctgcttgtcc gccttgcggc
     1501 tcttggtcgt cgaaccgccg gcgccgaacc cgtagatgct cgcctcggcc gacgcgttga
     1561 gctcgttgag cgagccgagc gcgaaggcgc cgaggttgta gccgtacacg aagtgcgtcg
     1621 cgccctcgca gccggggttg ctctggatgt cctcgcggta caccgcgtcg cgggtcgcgt
     1681 tgcgcgtgcc cgacacgtag tactccatgt ggaacttctc gccgccggcg atgcggccgc
     1741 cgagggtggc ggagctgagc gggagcttgg cgtgcagctc gccctggttg acgatgtcga
     1801 gcttctcgag cgcgccggag gtccactcga ccggcttgta ggcgccctgc gagccgcgga
     1861 tgctgtcgtt gcggcactcc tcgagcaccg tcatgtcaca gccctcgtag cggacgacga
     1921 cgatgtcgct cgcggcgtag gcctcgaagc tcgacttgtc ggtcgcgtcc cactcgatga
     1981 tgaaggggcg caggtggttc ttcgggttgc aggcgttctg cgcggcgaag ctctcgttca
     2041 tcaggccgct ttgcccggac tcgcgggtcg cggtcgtccg cgggatcttc gggccgcagg
     2101 acgcgagacc cagagagaga gagagaaggg acagcgggag cacggcacga agctgcatcg
     2161 atcgattcct gagagagcca gcgcgacccc aatgcgggcc gcaggcgcgg gagattgccc
     2221 gaacataaac gccgcgtcga gccccctcgc ccgcccgacg gacagcgatg gggctcgccg
     2281 gatcggcgcg gcgaaacgat gttcgagctc agccaaccat gcgcgaacga cggcgacgca
     2341 gcccgagccc gccgaacaac agcagccacg cccatgaaga aggagaagaa gacgacgacg
     2401 ccggtgtcac gcacgagcag cccttgtcgt cgccggtctc catgccggag tcgtcgctgt
     2461 cctgtgcggt gtcggcatcg gacgatgcac cgttgccgct ggacgcctcg ccgccgtcgc
     2521 cggacgactc gccgccggtc ggctcgctgg tgtcgctccc ggagccgtcg tcgctgtcgc
     2581 cggtggtcgg cgtgtcgccg gtcgtattct cctcgccggg cacggtgtcg acgaagatct
     2641 tggcctcggc gaagccgatg ttgcggtcgt ggtcgaccgc ctcgacgcgg atcgtgtaca
     2701 cgcccttggg cggcgcgggc aggtcccaca ccttctggcc gttgtagacc ggctgctcct
     2761 ggtcgagctc ggggaccatc aggcgccagc cgtagccctc gtagtcgtcg ccgacctcga
     2821 cctcgatgtg caccgtgtcg ccggccttgt actgctggcc gtcggtcggc gagaacatcg
     2881 tgacggtcgg cggcaccgtg tcggggctgt cggggccgaa gtaggccatc agctcggcga
     2941 cgtcgttctg cgcgccctcc gggcagaact gcttgtgtac atactggcag ccgatgccgc
     3001 cggtcgcgtc gttgtagacc gtgcaggtgt cggcccacac cttgttaccc ggcgtcgcgt
     3061 acgggtacat gatgttgttc tcgccgtcga tgtggtcgag gccccacgcg tgcgcggcct
     3121 cctgcagcgc ggtcgtcccg aggatgccga cgttgttggt ctcctcggtg aaggcgaagg
     3181 tcgtgtcgcg ccaccaggcg tcgccgcagt cgaggttgca cgcgaccccg agcacgccgt
     3241 tgggcagccc gatgatctgc gggctgccgc ccatcatcac ctggctgtac ggcaggtgct
     3301 tcggcggcgg ctcgtcgaag acgatgcgca ggccgaacgg cttggccgcg tccttgaaga
     3361 cctgaatgat cgccagcgcc gtctgctcgc tgccaccgaa gccggggtag tcgaccttgc
     3421 cctgcacgca cggcgactcg cccttggacg cgttgccgcc gctggtcagc ggaccgccga
     3481 agaagttgag gaaggtcgtc gcgcggtgga ccggctcgtc ccacgcctcg accggatcgc
     3541 tcggctgcgt cagcgtcggc tcgagcgccg acttgggcag cacctgattc cccttgagca
     3601 cctggccctg ccgctcgagc agctcgcgaa agcccgccgc gcgcgcctcg atctcctccg
     3661 gcgtcatcgc cggcgggtcg ttcaccggct tgccgaacag cgtgcgcggc tccgatccag
     3721 cgctggcctc cggcgcgaaa agcagcgcgg cggtcaccgg gacgagcaat ggggcgagca
     3781 gtgaggtgag acgggtgtgc aggcgagcca tgggggtccg atcgcaccgt aacacagacc
     3841 cccacccggg accgcgggac ttggcgcaca aaaacgaacg gcgactgacg acctctgacg
     3901 acctctggcg acctctggcg gggcattggc cgtcagtcgc cgccggtcgg gcagaccagc
     3961 ggatcctcgg cgtctccgac cacgcacagg accgcctcgg cgccgtccgg gggcgggcag
     4021 cacaggtgct tctcggcgtc cgaacctccg ccgctcgtgg tcgactcgac gatcccgctg
     4081 gcctccgggc atgcgtcgca ggggcacgcc gcgcagtcga cgggcaggat gtcgcggcac
     4141 tccgaggcga tgcagcgctc gtcggacttg cagtactcgt cgcagctccc gcagtgcgcg
     4201 tcgtcccgcg agaggtcgac gcacgcgtca ccgcagcgcg tcagaccctc gtcgcaggga
     4261 tcgccggtcg tttgtatgtc gccggtcgta cccgcggtgc tggtcggcgc tggatcgccc
     4321 gtcaccacgt gacccatgct gtcgtctgtg tcgccgcccg agctgtcgcc ctcgggcaac
     4381 acatactcga cgaggcagcc gccggtcatg aacgcgagcg cgatgagagt cgaacgcacg
     4441 tactgtcttt cccgcggccc ggcgactgtt acagaccgtt acacccgccc gtcgctcgtg
     4501 acaccaggct ccaccgggct ggcgtgacgg ccaggtcacg gcggggccgg ctccgcctgc
     4561 ttgggggcga gccccctcgc gcgggccacg agcgacaccc gggggcgcgg gaaagcgggc
     4621 gaaacgcgac gttgacagtc tttttgccac gtccgtagag tgcagtcatg gtggaagctc
     4681 cagacaaact ccggcggacc ggcggcctcg gcggcctcgg caacctcggc agcctcgcat
     4741 gttcgatggg actgctgctc gcgaccggct gttataccgg cgccgacctc gacggcgacg
     4801 acgacaccgt gaccggcacc gcgagcaacc ccagcggcgc cgacgccggt gaggacagca
     4861 cgccgacgac gggcgccacg gcgggcacgg cgggcactgc cggcacggcc gacaccggcg
     4921 acacggccga caccggcgac accgcgggtg acgcggacgg cctgccgcag acctcgcggt
     4981 atccgcgtct gagccacacg cagtgggaga acagcgtcca ggacctgttc tttttgccgg
     5041 agccgaccgg cttcagcgag ctcttcatcg gcgatccgat cagtggtggc ttcgacaaca
     5101 acagcgaggc gctcaaggtc accgcgacgc tgtggaccga ctaccagcgc ggcgcggagc
     5161 aggtcgccga cctcgtcgcc ggcaacccgg agatcctcgc caagatcgcg ccaccgaccg
     5221 agggtgactc ggacgcgcag gcgcgggcct tcatcatcgc cttcggcaag cgcgcctacc
     5281 ggcgcccgct gacgcccgag gaggtcgagc gccacctcca gatcttccag tccggcgtct
     5341 cgaagcccgg ggatctcgag cccttcgccg ccggcgtgcg gctcgtgctg caggcgatgt
     5401 tgcagtcgcc cttcttcgtc tatcgcgtcg aggcgagcga cgtcgacgac ggctccggca
     5461 agatcaagct cggtgactac gagctcgcca gcaagctgtc ctacatgctg tggaacacca
     5521 tgccggacga cgagctgttc gccgccgccg acgccaagca gctgcaggac gcgaccggcg
     5581 tcgccgccca ggcgacgcgc atgctcgagg acccccgcgc gcgcacgatg gtcgccacct
     5641 tccatcacca gctgctgcac gtcgaccact acagcgacgt gtacaaggac ccgaccaagt
     5701 tcccgaacta cgacccggcg atgaacgcga tgatggcgac cgagacgatg atgttcgtcg
     5761 aggacgtcgt cttcgggcaa aacggcgacc tggcgaccct gttgaccgcc ccttacagct
     5821 tcgtcaacga caagctcgcc gcgctctacg gcgtcgaggc gcccgccggc tccgagttcg
     5881 ccaaggtcga cctcgacccg gcgcagcgcg ccggcatctt gacgcagatc ggcttcctct
     5941 cggccaacgc gggcgccttc gagaacgaca tcatccaccg cggcgtgttc atgaacctgc
     6001 agctgatctg ctcgccgctg ccgccgccgc cgaacatggt cccgccgctg ccgcccccgg
     6061 tcgagggcga gaccctgcgc gagcgcgtcg accggcacac cggcgagggc acctgcggcg
     6121 cgggctgcca cggcggcatg atcaacccgc tcggcttcgc cttcgagaac tacggcccga
     6181 tgggacagtg gcagacgatg gacgccggcc agccggtcga cgccgccgcc gcgttccagt
     6241 tcaaggacgg cctcaagccc tacgacggcg ccgtcgagct cgcgcagctg atggccgaga
     6301 gccccgacgc gcaccgctgt tacgtcgcgc acctgctcga gtacggctac gcccgcggcc
     6361 cgcagaaggc cgacgacgcg ctgatccaga aggtcgccga ggcctcgcag gccggcaccc
     6421 tctcgatcaa acagatgatc ctcgagctga cccagagcga agccttcctg tacagggctc
     6481 cggtggaggg atgaccatgc ctcgcaagaa caacttctcc cgtcgcgcat tcctttacgg
     6541 cgccggtggc gtcaccgtcg cgctgccctt cctcgacgcc ctgcgcccgc gcaaggtcgt
     6601 cgccgccggg cccgaccgcc cgcgctacgc cgtgttcgtg cgccaggcca acggctgcgc
     6661 ccaggccgag ggcatggagc ccgagcagtt ctggccgcac gagctcggcc cggtgaccaa
     6721 ggagtcgctg agcaccaccg acgccgaccg tgcggtcggc gagctggccg agtacgccga
     6781 caagctgctg atggtccgcg gcacccgctt cccgttcccg ggcaacggct gcggccactc
     6841 gggcggcggc aaccaggtgt tgacggcgca gaaggtctcg atcgacccga tgggcaacaa
     6901 gtcgctggcg atgggcgagt cggtcgacaa ccgcatcgcg cgcgagctca acccgctgag
     6961 caaccccgag ccgctggcgg tctacgccgg ggcgatgggt ggttacatca acgaggtgct
     7021 gtcgtaccgc ggcgagaagg acctgcgggc ggccgagcgc aacccgtgga acgtctacca
     7081 gaagatggtc ggcatcacga tgttgcccga gcagcagatc atggacatca aggcgcggcg
     7141 caccagcgtc aacgacctgc tcaaggagca gatgaccgcg ctgatgtcct cgcccaagct
     7201 cagcagcgac gaccacgacc gcctgcagct gcacttcgac gcgatccgcg acatcgagat
     7261 cgagctcgcc tgctcgttgc ccccgcagga ggagctcgac gcgatggcgg cgcagatgga
     7321 ctacagcggc caggacgacc gcatgcagat catcgtgcag atgcactgca acctgatcgc
     7381 gctcgccttc gcctgcgact acacgcgcgc cgcgaccctg cagatcggcg acggcaacga
     7441 cggcaccgag tacctgatcg acggcaacaa gttcccgcgt taccaccaga tctcgcaccg
     7501 gatctacagc gacggcgccg agggcgaccc gatccccgac gcgctc
//
LOCUS       DASWNJ010000678         2592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_578021, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000678 DASWNJ010000000
VERSION     DASWNJ010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578021"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..127)
                     /locus_tag="VGB85_11360"
     CDS             complement(<1..127)
                     /locus_tag="VGB85_11360"
                     /inference="COORDINATES: protein motif:HMM:NF020118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HEY0134675.1"
                     /translation="MEEAVNLNTVLEQVCAEKNISREVLIETVEQAVATAAKKVFE"
     gene            complement(184..990)
                     /gene="rimP"
                     /locus_tag="VGB85_11365"
     CDS             complement(184..990)
                     /gene="rimP"
                     /locus_tag="VGB85_11365"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEY0134676.1"
                     /translation="MVAKTPSNRSKRPAAAPRDAVQQDAASEVSDSDTSVDTSEGFEE
                     QDDPADDTSETSESGPFHAASVRLLEDAVEPVARAMGYEVVLLEWVGGRGGRIARIYL
                     DHPNGITLDDCTRMSRIFSNALDAAEADPELPALAALLAQPYTLEVSSPGVDRPLVRL
                     SHFARQVGGRAVVRTHHPVVAGSDQKTFHGHIVGTEVEPAAPEDDRRGTVLLRALDGD
                     IIHSIALTDIKRANLVFELAGPQPRPSNGGTPGGKPAKAAKTPKHRDPAV"
     gene            1156..2049
                     /locus_tag="VGB85_11370"
     CDS             1156..2049
                     /locus_tag="VGB85_11370"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY0134677.1"
                     /translation="MRDIDAAVVQMCAGPDPEASLARAAELTAEALERGAGLVALPEN
                     YWGVAPPGFDRLAWAADLRGLGASGEIAGEPPAVLAAQLALSRGSDALIVLGGLPERP
                     GPDDGATEGTIFNTLAVLRRGKVVATYRKIHRFDATLQDGSKLQESASVSAGSHPTVL
                     DTPDACLGLSVCYDLRFPELYRALAAAGADILLVPSAFTLFTGLAHWEPLLRARAIEN
                     QAYVLAPAQHGPHGHGRHSYGHSMIVDPWGTVIAEASRGDGVAIARLRGEVLAQVRAD
                     LPALTHRRLSGETSVTRRSLP"
     gene            2046..>2592
                     /locus_tag="VGB85_11375"
     CDS             2046..>2592
                     /locus_tag="VGB85_11375"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY0134678.1"
                     /translation="MNAIDLTYPGSTGLAVLMDRYLDGDQKAFTALHAALTPRLRGLL
                     HKLVRDNATAEDLLQATLLKAHLAREGFVVRGSHADAAVQAWYSTIARNLALDFLRAQ
                     VRDRRRRADGSDERDPVEEIAADAPSIEEWQVDRDTAEEIAGRVHAALAGLPPAQREI
                     VEMHKLAGLSMAEIATRLQIRE"
BASE COUNT          350 a          890 c          958 g          394 t
ORIGIN      
        1 cttcgaacac cttcttggct gccgtcgcaa ccgcctgttc tacggtctcg atcaggacct
       61 cgcgcgagat gttcttctcg gcgcagacct gctcgaggac agtattgagg ttgactgctt
      121 cttccatggg gtctctttag cctctgccgt ggtcttctgg agcgggtccg cgtctactgc
      181 ggatcaaacc gccggatcgc ggtgcttggg ggtcttggct gcctttgcgg gcttgccccc
      241 gggggtcccc ccgttcgacg gtcggggttg aggcccggcc agctcgaaga cgaggttggc
      301 gcgcttgatg tcggtcagcg cgatcgagtg gatgatatca ccgtcgagcg cccgcaggag
      361 cacggtgcca cggcgatcgt cttcgggggc ggccggttcg acctcggtgc cgacgatgtg
      421 gccgtgaaaa gtcttttgat cggagccggc gacgacgggg tggtgggtcc ggacgacggc
      481 gcggccgccg acctggcggg cgaagtgact gaggcgcacg agcgggcggt cgacgcccgg
      541 cgaggacacc tcgagcgtgt atggctgggc cagcagcgcc gcgagggcgg gcagctccgg
      601 atcggcctcg gcggcgtcca gggcgttgct gaagatccgg ctcatgcgcg tgcagtcgtc
      661 gagggtgatg ccgttggggt ggtcgaggta gatccgggcg atgcgtccgc cgcggccgcc
      721 gacccactcg agcagcacga cctcgtagcc catcgcccgt gccaccggct cgacggcgtc
      781 ctcgaggagg cgcaccgagg cggcgtggaa cggcccggat tcgctggtct cggaggtgtc
      841 gtccgcgggg tcgtcctgct cctcgaagcc ctcgctcgtg tcgacgctcg tgtcggagtc
      901 actgacctcg gaggcggcgt cttgttggac ggcgtccctg ggggctgcgg cgggccgctt
      961 gctgcggttt gagggggtct ttgcgaccat agatggaaca gaaaaagcga gccgggcttg
     1021 atgcctgctc gctcgcgaaa ggcaaaatgc aggcgcttgg tagcacagcg cccaaacccg
     1081 agcaaccccc ctgacgcgcc ggccgccgtc ggtgtccgtg gccgagggcg agagtttggc
     1141 gctatgctgg cacccatgcg ggacatcgac gccgcggtgg tccagatgtg cgctggcccc
     1201 gatcccgagg ccagcctggc ccgtgccgcc gagctgaccg cggaggccct cgaacgcggc
     1261 gccgggctgg tggcgctgcc ggagaactac tggggggtcg ccccgccggg cttcgaccgc
     1321 ctggcctggg cggcggatct gcgggggctc ggggcctcgg gtgagatcgc gggtgagccg
     1381 cccgcggtgc tcgcggccca gctggcgctg tctcgcggca gcgacgcgct gatcgtgctc
     1441 ggcgggctgc cggagcggcc cgggcccgac gacggcgcga cggaagggac gatcttcaac
     1501 acgctggcgg tgctgcgccg cggcaaagtg gtcgcgacct atcgcaagat ccaccgcttc
     1561 gacgcgacgc tgcaggacgg gagtaaattg caggagtcgg ccagcgtgtc ggccggcagc
     1621 cacccgacgg tgctcgacac cccggacgcg tgcctgggcc tgagcgtctg ttacgacctg
     1681 cgcttcccgg agctgtaccg cgcgctcgcc gcggccggcg cggacatcct gctggtgccc
     1741 tcggccttca cgctgttcac cggcctggcc cactgggagc cgctgctgcg cgcgcgggcg
     1801 atcgaaaatc aggcctacgt gctcgcgccg gcccagcacg ggccgcacgg gcacggccgg
     1861 cacagctacg ggcacagcat gatcgtcgac ccgtggggca cggtgatcgc cgaggcctca
     1921 cgcggcgacg gggtggcgat cgctcgcctg cgcggcgagg tcctcgccca ggtccgggcc
     1981 gatctgccgg cgctgaccca ccggcgcctg tccggcgaaa catcggtgac ccggcggtcg
     2041 ttgccgtgaa cgccatcgac ctcacgtacc cggggtccac cgggctcgcg gtgttgatgg
     2101 accgctacct cgacggcgac caaaaggcct tcacggccct gcacgccgcc ctcaccccgc
     2161 gcctgcgagg cctcttgcac aagctcgtcc gcgacaacgc gaccgccgag gacctcctgc
     2221 aggcgaccct gctcaaggcc cacctcgcgc gcgagggctt cgtggtccgc ggcagccacg
     2281 cggacgcggc ggtccaggcc tggtactcga cgatcgcccg caacctcgcg ctcgacttcc
     2341 tgcgcgcgca ggtccgggac cgccggcgcc gcgcggacgg cagcgacgag cgcgacccgg
     2401 tcgaggagat cgccgccgac gcgccgtcga tcgaggagtg gcaggtcgac cgcgataccg
     2461 cggaggagat cgccggacgg gtccacgcgg cgctcgccgg gctgccgccg gcccagcggg
     2521 agatcgtcga gatgcacaag ctcgcggggc tcagtatggc ggagatcgcc acccgcctgc
     2581 agatccgtga gg
//
LOCUS       DASWNJ010000679         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_260673, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000679 DASWNJ010000000
VERSION     DASWNJ010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260673"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VGB85_11380"
     CDS             complement(<1..205)
                     /locus_tag="VGB85_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0134679.1"
                     /translation="MFSALIIDADGKTPATLKSLLAPYGFEFTVTENGPEAVNVARQA
                     APDIILLRAELPLTTGFSVCNRLR"
     gene            complement(657..>1509)
                     /locus_tag="VGB85_11385"
     CDS             complement(657..>1509)
                     /locus_tag="VGB85_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134680.1"
                     /translation="ARHRVISDPTDPRDPMPMASDSLIALIQPFLDDELPASERARVE
                     AVLAQDPVLRDMVDEQRSVRQALRDLPPEAAPQALQARVLLELDAVDREQAADPAPGW
                     RDRFGGRLRSFFRGAAVMVPAGATALALFLVTRTGAERHVPEASPASAPVTAAAPTLL
                     DPSRSGLVLGAAPGASPGVRLVGAALPTGDRPPSSVIVERQVGPRRVLDRHEPAGGPP
                     PSEPHAYRGRSYWLGHVGGQPAVAFESDGVRHTLTADTRESAGFRQDREYAFLLSLGD
                     ALQEAER"
BASE COUNT          201 a          495 c          617 g          196 t
ORIGIN      
        1 gccgcaggcg gttgcacacc gagaagccgg tcgtcagcgg cagctcggcg cgcaggagga
       61 tgatgtctgg cgcggcctgt cgcgcgacgt tgacggcctc gggcccgttc tcggtgaccg
      121 tgaactcgaa gccgtacggc gcgaggaggc tcttgagagt ggcgggggtc ttcccgtcag
      181 cgtcgatgat cagcgcagag aacacggacg gcaggttatc cgacctcgca ctgccatggc
      241 cagagacgcg ccgaaatcgc ggccggtcgt gtcggcgacc cgggccccgc gaggccgcca
      301 ccgggtgtgt cgcgggtggt caatcaccgg ggaatcaccg tcgatgtgag gccgtcgggg
      361 ccgcagcagg cccgggggcg attttgcgcc tggtgccggc ggggctgcgg gccgggccag
      421 ctgtacgagt tcccaattaa tcgtcatgta ccgggacgaa ggaggatgcg ccgcggggaa
      481 tgacccggcg cggcgccaag gagtcatgcg tcctgtcctc accggtcgcg accaggcgga
      541 tgcacgccgg ccgatggggg cggcggaccg gaccccgtga ctggcgtggc tgactcaccc
      601 cacgtcgtgt cgacacgaga tcggcgggaa cgtgggactt cgtggcgcgc gagcgcctag
      661 cgctctgctt cctgcagggc gtcgccgagc gacagcagga aggcatactc gcggtcctgg
      721 cgaaagccgg ccgactcgcg ggtgtcggcg gtcagggtgt ggcgcacgcc gtcggactcg
      781 aaggcgaccg ccggctgccc gccgacgtgg ccgagccagt agctgcggcc gcggtaggcg
      841 tgcggctcgc tcggaggcgg gccgccggcg ggctcgtggc ggtcgagcac gcgacgcggg
      901 ccgacctgac gctcgacgat gacggagctc ggcgggcggt ccccggtcgg cagcgcggcg
      961 ccgacgaggc gcacgccggg gctggccccg ggggcggcgc cgaggaccag gccgctgcgc
     1021 gacgggtcga gcagcgtcgg cgcggcggcg gtgaccgggg cgctggccgg gctggcctct
     1081 gggacatgtc tctcggcacc tgtcctcgtg accaggaaca gcgcgagcgc ggtggcgccg
     1141 gcggggacca tgacggcggc gccgcggaag aacgatcgca ggcggccgcc gaagcggtcg
     1201 cgccagccgg gcgccgggtc ggcggcctgc tcgcggtcga cggcgtcgag ctcgagcagc
     1261 acgcgggcct gcagcgcctg cggggcggcc tcgggcggca ggtcgcgcag cgcctggcgc
     1321 acgctgcgtt gctcgtcgac catgtcccga aggacaggat cttgcgcgag cacggcctcg
     1381 acgcgcgcgc gctcgctggc cggcagctcg tcgtcgagga agggctggat cagggcgatg
     1441 aggctgtcgc tggccatggg catcggatct cgagggtcgg tgggatcgct gatcacgcgg
     1501 tgcctcgct
//
LOCUS       DASWNJ010000680         2230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_224681, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000680 DASWNJ010000000
VERSION     DASWNJ010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224681"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /locus_tag="VGB85_11390"
     CDS             complement(<1..1142)
                     /locus_tag="VGB85_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134681.1"
                     /translation="MSVPFSPSSLPALALALALAGCGGGGGGGGGEEGGGSSTGGDTE
                     AAPDNDAEVPSEDCAGAPSVAQGSFAGNLRGRTPDPVIGGVCGGGGPDVFLRVEVPLR
                     ADLRIEARGNGFTPRVSLAPVGCQPAPMLTCGANGVVELADLEAGTMVNLAVGVDAET
                     LATLGELPLPDDGVDPLGFVVDVGMTRVLARGEPCRPAARGRCAAGSLCLQQVLVADT
                     DDTDDTDDIDDEADEVWSCTPLEGDHCIDPEWAAVQLVDGEGTLLVDPDLPQSDAHHH
                     SCTGEGTRERVIRLRLPGGLGVHSSLQIRTERPEIGLALRAPGCLASEETACARPSPA
                     GSQVTIVAPDELQREGIEPYLFVEMPEPGVLAEPVILHLRRVLHAPP"
     gene            1229..>2230
                     /gene="hisD"
                     /locus_tag="VGB85_11395"
     CDS             1229..>2230
                     /gene="hisD"
                     /locus_tag="VGB85_11395"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY0134682.1"
                     /translation="MAGPSPDVLDLRPPADGAGHDRWRRLCARGAGLEGEADVAAREV
                     IAAVRERGDAAVSELTERYEGRHIADFELSPQARRAAAAEIPADLRDALGLAASNIRA
                     FHETQRSGDTGLHRGDLRLTSRVRPLARVAVYAPGGTAAYPSSVLMTAIPAKVAGVPE
                     VILFTPRPSSVVLLAAELAQVDRVFAVGGAQAIAAAALGTAQIPRVDKIVGPGNAYVT
                     AAKRQVFGLVDIDGIAGPSEILVLADANADPVLIAADLLAQAEHDTLACAVVVTDAPA
                     LVPKIHAAIAAQLEDLPRREIAARALADHGAIVLVADRDAMVAAANAYAPEHLELLVD
                     "
BASE COUNT          290 a          933 c          730 g          277 t
ORIGIN      
        1 gggggtgcgt gcagcacccg tcgcaggtgc aagatcaccg gctcggcgag cacgccaggc
       61 tcgggcatct cgacgaacag gtacggctcg atgccctcgc gttgcagctc gtcgggcgcc
      121 acgatcgtca cctgggagcc cgcgggcgag ggccgggcac aggccgtctc ctcgctcgcc
      181 aggcagcccg gcgcgcgcaa cgcgaggccg atctccggcc gctccgtccg gatctgcagg
      241 ctgctgtgca cccccaagcc gccgggcagg cgcagccgga tcacccgctc acgcgtgccc
      301 tcgccggtgc agctgtggtg atgcgcgtcg gactggggca ggtcggggtc gacgagcaac
      361 gtgccctcgc cgtcgaccag ctgcaccgcc gcccactcgg ggtcgatgca gtggtcaccc
      421 tccagcggcg tgcacgacca cacctcgtcc gcctcgtcat cgatgtcgtc tgtatcgtcc
      481 gtgtcgtccg tgtccgcgac gagcacctgt tgcaggcaca gcgagcccgc cgcgcagcga
      541 ccccgcgccg ccggccgaca cggctcgccg cgcgcgagca cccgcgtcat gccgacgtcg
      601 accacgaagc cgagcgggtc gacgccgtcg tctggcagcg gcagctcgcc gagcgtcgct
      661 aaggtctccg cgtcgacccc gaccgcgagg ttgaccatcg tgcccgcctc gaggtcggcc
      721 agctcgacca cgccgttcgc cccgcaggtc aacatcggcg ccggctggca gccgaccggc
      781 gccaggctga cccgcggcgt gaagccgttg ccgcgcgcct cgatccgcag gtcggcgcgc
      841 agcgggacct cgacgcgcag gaacacgtcc ggtccgccgc ccccacagac cccaccgatc
      901 accgggtcag gcgtgcgacc gcgcaggttg ccggcgaagc tgccctgggc gaccgacggc
      961 gcgcccgcgc agtcctcgct cggcacctcc gcgtcattgt ccggcgcagc ctccgtatcg
     1021 ccgccggtcg acgatccgcc gccctcctcg ccgcctccgc ccccgccgcc cccgccacag
     1081 cccgccagcg ccagcgccag cgccagcgca ggcaaggagg acggtgagaa gggcacggac
     1141 atgggcgatg ttacggacag cccatcgtag cggatcctcc cgccggtttc acttccgccg
     1201 aaacggccca cgctgtagct ttccctccat ggccggccca agccccgatg tcctcgacct
     1261 ccgccccccc gcggatggcg ccggtcacga caggtggcgt cggctctgtg cccgcggggc
     1321 cggcctcgag ggtgaggccg acgtcgccgc ccgcgaggtg atcgccgccg tacgcgagcg
     1381 cggcgacgcg gccgtcagcg agctgaccga gcgctacgag ggccgccaca tcgccgactt
     1441 cgagctctcg ccgcaggccc gccgagccgc cgcggccgag atccctgcgg acctccgcga
     1501 cgccctgggg ctcgccgcga gcaacatccg cgccttccac gagacccagc gcagcggcga
     1561 caccggcctg caccgcggcg acctgcgcct gaccagccgc gtgcgcccgc tcgcgcgcgt
     1621 cgccgtctac gccccgggtg gcaccgccgc ctacccgtcc tcggtcctga tgacggcgat
     1681 cccggccaag gtcgccggtg tgcccgaggt gatcctcttc accccgcgcc cctcgagcgt
     1741 cgtcctgctc gccgcggagc tggcccaggt cgaccgcgtg ttcgccgtcg gcggcgccca
     1801 ggcgatcgcc gccgccgctc tcggcaccgc ccagatcccc cgcgtcgaca agatcgtcgg
     1861 ccccggcaac gcctacgtca ccgccgccaa gcgccaggtc ttcggtctcg tcgacatcga
     1921 cggcatcgcc ggcccgagcg agatcctcgt gctcgccgac gcgaacgccg acccggtcct
     1981 gatcgccgcc gacctgctcg cccaggccga gcacgacacc ctcgcctgcg ccgtcgtcgt
     2041 caccgacgcg cccgcgctcg tcccgaagat ccacgccgcg atcgcggccc agctggagga
     2101 cctcccgcgg cgcgagatcg ccgcgcgggc cctcgccgat cacggcgcga tcgtcctcgt
     2161 cgccgaccgt gacgccatgg tcgccgccgc caacgcctac gcgcccgagc acctcgagct
     2221 gctcgtcgac
//
LOCUS       DASWNJ010000681         1776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_189449, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000681 DASWNJ010000000
VERSION     DASWNJ010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189449"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VGB85_11400"
     CDS             complement(<1..534)
                     /locus_tag="VGB85_11400"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HEY0134683.1"
                     /translation="MTTSRRIPWLLSLTVLTACPPDTKDTPTEGSSETSTGAATEPTT
                     GVPTGSDSSSAAETDPGDTQPDETDTDAAVCGEPALDQVIATGKPRARAGHAVAGAGD
                     VNGDGVPDVIIGAPGGPSAADPNEPGRAFVVFGPAQQAELALGETQGLTLVGEAAHQI
                     AGEVVEGIGDVNGDGRDD"
     gene            complement(633..1094)
                     /locus_tag="VGB85_11405"
     CDS             complement(633..1094)
                     /locus_tag="VGB85_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134684.1"
                     /translation="MTLWAAVLALGCSAAPRDTDPTTEGETSGGTTDGSVTTGGPAVT
                     TGQASTGPDEDGTTSGTDSDTGGEPSPECVLYCDEFMPNCSEIANVEVYDDLADCQST
                     CAAFTHGPVGEFMGDTVECRIAHLTFDPAKPPGYYETHCFHAQEHPTANCV"
     gene            complement(1150..>1776)
                     /locus_tag="VGB85_11410"
     CDS             complement(1150..>1776)
                     /locus_tag="VGB85_11410"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0134685.1"
                     /translation="DFDYEGRTTLPLASADERGAVLYVGSLSKVLAPGLRIGYAVAPR
                     PVLELMSERRAFIDRQGDHVLDYAVAELIEEGQLRRHVQRMRAIYAARRAALAEALTG
                     MLGAVVTFRLPERGTAIWATVRDDIDVQAWHDRARQRGVTFSTGRSFRFDDAPTAHAR
                     FGFAGLDEPQLREAVKRLRAALPDPRVRGRPVRATIREPDLLRTDADT"
BASE COUNT          231 a          661 c          620 g          264 t
ORIGIN      
        1 gtcgtcgcgg ccgtcgccgt tgacgtcgcc gatgccctcg accacctcgc cggcgatctg
       61 gtgcgccgcc tcgccgacga gcgtcagccc ctgcgtctca ccgagcgcga gctcggcctg
      121 ctgcgccgga ccgaacacca cgaacgcgcg cccgggctcg ttcgggtcgg ccgcggacgg
      181 cccgccgggc gcgccgatga tgacatcagg cacaccgtca ccgttgacgt cacccgcgcc
      241 cgcgacggcg tggccggccc gggctctcgg cttgccggtg gcgatcacct ggtcgagcgc
      301 cggctctccg cacaccgccg cgtcggtgtc tgtctcgtcc ggctgcgtgt cgccgggatc
      361 ggtctccgcc gcgctcgatg aatcgctgcc cgtgggcacg ccggtggtcg gctcggtcgc
      421 ggccccggtc gacgtctcac tggagccctc ggtcggggtg tccttcgtgt ccggcggaca
      481 ggcggtcagc accgtcagcg aaagcagcca tgggatgcgg cgagaagtcg tcatgcgccg
      541 cgcctgagca agtcgcgtgc cagcgacatt cactgccgtt tcggcgcctc gacccacccc
      601 tccatgacgg caatgtcgcg ctcgccggac gactacacgc agttggccgt cggatgctcc
      661 tgcgcatgga agcagtgcgt ctcgtagtac ccgggcggct tcgccggatc gaaggtgaga
      721 tgcgcgatgc ggcactccac cgtgtcgccc atgaactcgc cgacgggacc gtgggtgaac
      781 gcggcgcacg tgctctgaca gtcggcgagg tcgtcgtaga cctcgacgtt ggcgatctcg
      841 ctgcagttgg gcatgaactc gtcgcagtag agcacgcact cgggcgacgg ctccccgccg
      901 gtgtcgctgt ccgtgcccga cgtcgtgccg tcctcgtccg ggcccgtgct cgcctggccc
      961 gtcgtcacgg ccgggccgcc ggtcgtgacc gacccgtccg tcgtcccccc ggacgtctcg
     1021 ccctcggtcg tcgggtccgt gtccctgggc gcggcggagc acccgagcgc gagcacggcg
     1081 gcccacagag tcattcgcaa gtccatcatg accatccttc gccggacctg cgcggtccgg
     1141 acgtgtggtc tacgtgtcgg cgtcggtccg gagaagatcc ggttcgcgaa tagtcgcgcg
     1201 taccggccgg ccccggaccc gcggatccgg cagcgctgcg cgcaggcgct tgacggcctc
     1261 gcgcagctgc ggctcgtcga gcccggcgaa gccgaagcgg gcgtgggccg tcggcgcgtc
     1321 gtcgaagcgg aacgaacgcc cggtgctgaa ggtgaccccg cgctgccgcg cccggtcgtg
     1381 ccaggcctgc acgtcgatgt cgtcgcgcac ggtcgcccag atcgcggtgc cccgctcggg
     1441 cagacggaac gtgaccacgg cgccgagcat cccggtcagc gcctcggcca gggccgcgcg
     1501 gcgggcggcg tagatcgccc gcatgcgctg cacgtggcga cgcagctgac cctcctcgat
     1561 cagctcggcc accgcatagt cgagcacatg atcgccctga cgatcgatga aggcgcgacg
     1621 ctcggacatc agctccagca ccggccgcgg cgcaaccgca tagccgatgc gcaggccggg
     1681 cgcgagcacc ttggacagcg agccgacgta gagcaccgcg ccacgctcgt cggcgctcgc
     1741 cagcggcagc gtggtccggc cctcgtagtc gaagtc
//
LOCUS       DASWNJ010000682         3304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_648235, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000682 DASWNJ010000000
VERSION     DASWNJ010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648235"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1994
                     /locus_tag="VGB85_11415"
     CDS             <1..1994
                     /locus_tag="VGB85_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134686.1"
                     /translation="QGMTLDVERVLEIQTAASPSLVPGSGEIVFEQVDAHDLRYSWNE
                     LYLWRPSEPGATDVRQSPSEVVQLTSGMRARDPHVASDGRSVVFVRNDNAQSRLAFID
                     LHTREVVELAPTDRVQQIYSPRFAPDGRKVAYSAHRQGYRDIYLYDRDSGKTTRITAD
                     RFLDMNPAFSADGRYLLFSSDRDDIFNIYAHDLETGVLSQVTNVIGGAFDPVVSHDGT
                     RLAYVGFSSTGYDLWAMKLDPAEFFAPLPVQDDLPQADDPTPELPEDHGRPPALRSRR
                     YQPLRTFYPRVLLPASFEFGNNGQGADLGIKLDITDVVGFHRLSASFRDYLRYDVPVG
                     SVGYTYSQLLPSFSIGFSRDFRAYDNGRIRYDYDNEGAGGAFEPYRVTGYRERQTIVR
                     AGVSVPVLRHPIHQANAGVTYEFARLRDLDEGREVVDPNAPTSVPPVVGDTGRVTLGL
                     TYASLRGVRYGYGEETGRSAGLRLDIIDRHLGGQYGDIQVSANYGERIRMPWRGHQVL
                     ALRLAGGASAGGLGGLGAFRLYGTPQPNSVFQDFLQRNAWGEAGTLRGFTPAGFAGTY
                     YLVLNSEYRIPLADVERGMSSLPLFLRRVTAIPFLDYGGAWSGKLTRDLLKFGAGASL
                     VFSFRIGYRETIDLFVTYAHGFDPQRGADVLRVLVARSF"
     gene            2036..>3304
                     /locus_tag="VGB85_11420"
     CDS             2036..>3304
                     /locus_tag="VGB85_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134687.1"
                     /translation="MPALRLASALPARRRRGPAAVSLALLAWILLAPQDARANSRDLK
                     WRTLQTTHFVIHYHQGEEDAAERASRLLERAHARLSVGLGHEPFLRTQVVIVDNTDEA
                     NGVANAVPFPRLTANLTAPNSMSVLESYDDWLDILLTHEYTHVVHLDTVHGIPRLVNA
                     VLGFGVLGKVWSPNIIQPRWMVEGLASYEESRLGSQGRRRSAQFDMMLRMAVLEHGFL
                     PIDRVSSGANVFPHGTSAYLYGLHFMSYIGTRYGHDKLRELSHVFGGQAIPFGINRAM
                     SKVLGVDFEQLWEEFELDTTRRFQAQARTIRGRGIREGRRLTYSTTSAASGQFNRHPF
                     WSPDDEYLYFYQDDGHSNPGIRKLPAIGGRIREGVGIGRQGMNLDIERVVEVQDAASG
                     SFVLSTSDLVFEVTQVHDFRYSWSDLVLWHG"
BASE COUNT          473 a         1262 c         1058 g          511 t
ORIGIN      
        1 gccagggcat gaccctcgac gtcgagcgcg tgctcgagat ccagaccgcg gccagcccga
       61 gcctcgtgcc cggctccggt gagatcgtgt tcgagcaggt cgacgcccac gacctgcgct
      121 acagctggaa cgagctgtac ctgtggcgcc cctccgagcc cggcgccacc gacgtgcgcc
      181 agagcccgtc ggaggtcgtg cagctgacct ccggcatgcg cgcccgcgac ccccacgtcg
      241 ccagcgacgg ccgctccgtc gtgttcgtgc gcaacgacaa cgcccagtcg cgcctcgcct
      301 tcatcgacct gcacacccgc gaggtcgtcg agctggcccc gaccgaccgc gtgcagcaga
      361 tctacagccc gcgcttcgcc ccggacggcc gcaaggtcgc ctactcggcg caccgccagg
      421 gctatcgcga catttatctg tacgaccgcg acagcggcaa gacgacgcgc atcaccgccg
      481 accgcttcct cgacatgaac ccggcgttct cggccgacgg acgctacctg ctgttctcga
      541 gcgaccgcga cgacatcttc aacatctacg cccacgacct cgagacgggc gtgctctcgc
      601 aggtgaccaa cgtgatcggc ggcgccttcg atccggtcgt cagccacgac ggcacgcgcc
      661 tcgcctacgt cggcttctcg tcgaccggct acgacctgtg ggcgatgaag ctcgaccccg
      721 ccgagttctt cgcgcccttg ccggtgcagg acgatctccc gcaggccgac gacccgaccc
      781 ccgagctgcc ggaggatcac ggccggccgc ccgcgctgcg ttcgcggcgc taccagccgc
      841 tgcgcacctt ctatccgcgg gtgctgctgc cggcctcgtt cgagttcggc aacaacgggc
      901 agggcgccga cctcgggatc aagctcgaca tcaccgacgt cgtcggcttt caccgcctga
      961 gcgcgagctt tcgcgactac ctgcgctacg acgtgccggt cggctcggtc ggctacacct
     1021 acagccagct gctgccgagc ttcagcatcg gcttttcgcg cgactttcgg gcctacgaca
     1081 acggccggat ccgctacgac tacgacaacg agggcgccgg cggggccttc gagccgtacc
     1141 gcgtcaccgg ctaccgcgag cgccagacga tcgtgcgcgc cggcgtgtcg gtgccggtcc
     1201 tgcgccaccc gatccaccag gccaacgccg gcgtcaccta cgagttcgcg cgcctgcgcg
     1261 acctcgacga gggccgcgag gtcgtcgacc ccaacgcgcc cacctcggtg ccgccggtcg
     1321 tcggcgacac cggccgcgtc accctcggcc tcacctacgc gagcctgcgc ggcgtccgct
     1381 acggctacgg ggaggagacc gggcgcagcg ctggcctgcg cctcgacatc atcgaccgcc
     1441 acctcggcgg gcagtatggc gacattcagg tctcggccaa ctacggcgag cgcatccgca
     1501 tgccgtggcg cgggcaccag gtgctcgcgc tgcggctcgc cggcggggcc tcggccggcg
     1561 gcctcggcgg cctcggtgcg tttcgcctgt acggcacgcc gcagcccaac agcgtgttcc
     1621 aggacttcct ccagcgcaac gcctggggcg aggccggcac gctccgcggc ttcacgccgg
     1681 ccggcttcgc cggcacctac tacctcgtgc tcaacagcga gtaccggatc ccgctcgcgg
     1741 acgtcgagcg cggcatgagc tcgctgccgc tgttcctgcg ccgcgtcacc gcgatcccct
     1801 tcctcgacta cggcggggcg tggtccggca agctgacccg cgacctgctc aagttcggcg
     1861 ccggcgcctc gctggtgttc tcgtttcgca tcggctaccg cgagacgatc gacctgtttg
     1921 tcacctacgc ccacggcttc gacccgcagc ggggcgccga tgtgctccgg gtcctggtcg
     1981 cccgcagctt ttgagcttca cgggcccggg gcccccctgt taagctgacg gtctcgtgcc
     2041 cgccctccgc ctcgcctccg cgctcccggc ccgccgccgg cgcggccccg cggcggtgtc
     2101 gctggcgctg ctcgcctgga tcctgctcgc cccgcaggac gcgcgcgcga acagccgcga
     2161 cctcaagtgg cgcacgctgc agacgacaca cttcgtcatt cactaccacc agggcgagga
     2221 ggacgccgcc gaacgggcct cgcgcctgct cgagcgggcc cacgcgcgcc tgagcgtcgg
     2281 cctcgggcac gagccgtttt tgcgcacgca ggtcgtcatc gtcgacaaca ccgacgaggc
     2341 gaacggcgtc gccaacgcgg tgccgttccc gcggctgacc gccaacctga ccgcgccgaa
     2401 ctcgatgtcg gtgctcgaga gctacgacga ctggctcgac atcctgctga cccacgagta
     2461 cacgcacgtc gtgcacctcg acaccgtgca cggcatccca cggctggtga acgcggtgct
     2521 cggcttcggc gtgctcggca aggtgtggtc gcccaacatc atccagccgc gctggatggt
     2581 cgagggcctc gcgtcctacg aggagtcgcg gctcggctcg cagggccgca ggcgctcggc
     2641 gcagttcgac atgatgctgc gcatggcggt gctcgagcac ggatttttgc cgatcgaccg
     2701 cgtctccagc ggcgccaacg tgttcccgca cggcacctcg gcctatctgt acggcctgca
     2761 cttcatgagc tacatcggca cgcgttacgg ccacgacaaa ttgcgcgagc tgagccacgt
     2821 gttcggcggt caggcgatcc ccttcgggat caaccgcgcg atgtcgaagg tgctcggcgt
     2881 cgacttcgag cagctgtggg aggagttcga gctcgacacc acgcggcgct tccaggccca
     2941 ggcccgtacg atccgcggac gggggatccg cgagggccgg cgcctgacgt actcgaccac
     3001 ctcggccgcc tcggggcagt tcaaccgcca cccgttctgg tcgcccgacg acgagtacct
     3061 gtacttttat caggacgacg gccacagcaa ccccggcatc cgcaagctcc cggcgatcgg
     3121 cgggcggatc cgcgagggcg tcggcatcgg ccgccagggc atgaacctcg acatcgagcg
     3181 cgtcgtcgag gtccaggacg ccgcctccgg gtcgttcgtg ctgtcgacct ccgacctggt
     3241 cttcgaggtc acgcaggtcc acgactttcg ctactcgtgg agcgacctgg tgctgtggca
     3301 cggc
//
LOCUS       DASWNJ010000683         2510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_437505, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000683 DASWNJ010000000
VERSION     DASWNJ010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437505"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VGB85_11425"
     CDS             complement(<1..902)
                     /locus_tag="VGB85_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0134688.1"
                     /translation="MKSDLFNPSDSHGPHRFHSDELESLVALAQAQPLPRLRTDADRL
                     HAGFLAARQARTRRLSLVGGVALAAGLAGLMVVRADLGADHATSPIGHVAQDMSPEPA
                     SSAVVEAPRLATGVRVAARGEATPPTVRGPWEVELAPGDYEAEVEPHPGPEVLRARAA
                     GGTVELHHGRVEIAVAASEAHATLRDGVATWVAPDGKRSELKTAAAAAKPTVEPADAG
                     TDVSDVRALARRAEALLTAGQRDRAIAVLTQITRVHPHEPAARAALLDLAPLLKAAGR
                     VDEARCAYRLYLERYPGKAQLADDV"
     gene            complement(899..1465)
                     /locus_tag="VGB85_11430"
     CDS             complement(899..1465)
                     /locus_tag="VGB85_11430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0134689.1"
                     /translation="MATQDEYSDESQVLQAAQRGDKAAQSQIFTRYRDRVARQLLRMV
                     GDPMAVDDLLQEVFIAAFTALPRFRGDAQLATWLYAIATNRTRNWWDSQRRRRRREDI
                     AANQPPEETSTPEADLESQQQRARFYAALGQLPDKLREAFVARAIEGMNLIEASAALE
                     VPISTVSYRTRRAEQLLCAALGIPWRES"
     gene            complement(1560..>2510)
                     /locus_tag="VGB85_11435"
     CDS             complement(1560..>2510)
                     /locus_tag="VGB85_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134690.1"
                     /translation="PRRLVKGRDSWHSLRPVPPPLAVLAVLVSLAAPQLDLRATSIDP
                     VATATGLHARVGPLDDWTIRVEDGAAPASVQMTLRSPDGRQQQRSVALTGTTNEDRSR
                     ELAASLALVIEQWDDPPPPDVEVLPPANEPPQKDPPQKDPPQKDPGPPPPAAAEPAAP
                     VRGWLGLGPRLGVGRALVEGGLDLQGGAWLAREHVQPLASLGWSATARDGLSLHTLRI
                     GAGLAIGAPVLTGRLWIGGHALTHAAWTRVHDAHTASVWSNSTEVGGLLQVRGGRWIA
                     GVRTGVELYLPQLRAHGSLARIDRGPALWFIGLNFGFMFG"
BASE COUNT          315 a          846 c         1008 g          341 t
ORIGIN      
        1 acatcgtcgg cgagctgggc cttgcccggg tagcgctcga ggtacagccg ataggcgcag
       61 cgggcctcgt cgacgcgacc ggcggccttg agcagcgggg cgaggtcgag gagggcggcc
      121 cgggccgcgg gctcatgggg atgcacgcgg gtgatctggg tcagcacggc gatcgcacgg
      181 tcgcgctggc cggcggtcag cagcgcctcg gcccggcggg cgagggcgcg gacatccgac
      241 acgtccgtcc cggcgtcggc cggctcgacc gtgggcttcg cggcggccgc ggcggtcttc
      301 agctcgctgc gcttgccgtc gggggcgacc caggtggcga cgccatctcg cagggtcgcg
      361 tgcgcctcgc tggcggcgac ggcgatctcg acgcggccgt ggtgtaactc gacggtgccg
      421 ccggcggcgc gggctcgcag gacctcgggg ccggggtgcg gctcgacctc ggcctcgtag
      481 tcaccgggcg cgagctcgac ctcccacggg ccgcgcacgg tcggcggggt cgcctcgccc
      541 cgggccgcca cgcgcacgcc ggtcgccagg cgcggggcct cgacgacggc gctgctggct
      601 ggctccgggg acatgtcctg tgcgacatgt ccgatcgggc tggtcgcgtg atctgctccg
      661 aggtcggcgc ggacgaccat cagcccggcg aggccggccg cgagggcgac gccgccgacg
      721 agcgacagcc ggcgggtccg ggcctggcgg gcggcgagga agccggcgtg caggcggtcg
      781 gcgtcggtgc gcagccgcgg caggggttgg gcctgcgcga gggcgacgag gctctcgagc
      841 tcgtcgctgt ggaagcgatg cggaccgtgc gagtcggacg ggttgaacag gtcggacttc
      901 atgactccct ccaggggatg ccgagggccg cgcagagcag ctgttcggcg cggcgagtgc
      961 ggtacgagac cgtgctgatg gggacctcga gggcggccga cgcctcgatc aggttcatgc
     1021 cttcgatcgc gcgggcgacg aaggcttcgc gcagcttgtc ggggagctgc ccgagcgccg
     1081 cgtagaagcg ggcgcgctgc tgctgggact cgaggtcggc ctcgggggtc gaggtctcct
     1141 cgggaggctg gttggcggcg atgtcctcac ggcgccggcg acgacgctgc gagtcccacc
     1201 agttacgggt gcggttggtg gcgatcgcgt acagccaggt cgcgagctgg gcgtcgccgc
     1261 gaaaccgcgg cagggcggtg aaggccgcga tgaagacctc ttgcaggaga tcgtcgacgg
     1321 ccatcgggtc gccgaccatg cgcagcagct ggcgggcgac ccggtcgcga tagcgggtga
     1381 agatctgcga ctgcgccgcc ttgtcgccgc gctgagccgc ctggagcacc tgcgactcgt
     1441 ccgagtactc gtcctgggtc gccatcgccc gcgagagtgt aacggaggag ctcactgcat
     1501 catcgaccgg gccgcggctc cggattacca atgtcgatgc gacccccccg cacctttctt
     1561 catccgaaca tgaatccgaa gttgaggcca atgaaccaca gggccggccc ccgatcgatg
     1621 cgcgcgaggc ttccgtgggc gcgtagttgt ggcaggtaaa gctcgacgcc ggtgcgtacg
     1681 ccggcgatcc agcgcccgcc gcggacctgc aacaggccgc cgacctcggt gctgttcgac
     1741 cacacgctcg cggtgtgcgc gtcgtggaca cgcgtccagg ccgcgtgcgt cagcgcatgg
     1801 ccgccgatcc acagccgccc ggtcaacacc ggggcgccga tcgcgaggcc ggcgccgatc
     1861 cggagggtgt gcaggctcag gccgtcgcgc gcggtcgccg accagccgag gctggcgagc
     1921 ggttgcacgt gttcgcgggc cagccacgcg ccgccctgga ggtcgagtcc gccctcgacg
     1981 agggcccggc cgaccccgag ccggggaccg aggccgagcc agccgcggac cggcgcagcg
     2041 ggctccgcgg cggcgggcgg gggcgggccg ggatccttct gtggcggatc cttttgtggc
     2101 ggatccttct gtggcggctc gttcgctggc ggcaggacct cgacatcggg cggaggtgga
     2161 tcgtcccact gctcgatgac gagggcgagc gacgcggcca gctcgcggct gcggtcctcg
     2221 ttggtcgtgc cggtcaaggc gacggagcgc tgctgctggc ggccgtcggg gctgcgcaag
     2281 gtcatctgca cgctggccgg cgcggcgccg tcttcgacgc ggatcgtcca gtcgtcgagc
     2341 gggccgacgc gcgcgtgcag gccggtggcg gtggcgaccg ggtcgatgct cgtcgcgcgc
     2401 aggtcgagct ggggcgccgc cagcgagacg aggacggcga ggacggcgag aggcggcggc
     2461 accggccgca aggagtgcca cgagtcccgg cccttgacca gcctccgtgg
//
LOCUS       DASWNJ010000684         2827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_118421, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000684 DASWNJ010000000
VERSION     DASWNJ010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118421"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VGB85_11440"
     CDS             <1..215
                     /locus_tag="VGB85_11440"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0134691.1"
                     /translation="EMMAKGTYGWILLATPDLDGTFEKLQAGDAEVVQEPTEQPYGVR
                     DCAVRDPAGNLIRIQEQRRTARRSQA"
     gene            231..2786
                     /locus_tag="VGB85_11445"
     CDS             231..2786
                     /locus_tag="VGB85_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY0134692.1"
                     /translation="MATKTDTKSPELHPADSHARIRVHGARVNNLKDISLDIPKRRLT
                     VFTGVSGSGKSSLVFGTIAAESQRLINETYSAFVQGFMPTLARPDVDVLDGLTTAILV
                     DQERMGANARSTVGTATDANAMLRIVFSRLGKPHIGSPQAFSFNVASISGAGAVTLER
                     GGKTVKERRSFSITGGMCPRCEGRGAVTDIDVSALYDDSKSLNEGAITIPGYTMEGWY
                     GRIFRGCGYFDPDKPIRKFNKKELHDLLHREPTKIKIDGINLTYEGLIPKIQSSFLAK
                     DVDAMQPHVRAFVERAVTFTTCPECEGTRLSKEARSSKIKGVNIADVCAMQISDLAEW
                     VRGLREPSVAPLLANLQQVLDSFVEIGLGYLSLERPSGTLSGGEAQRVKMIRHLGSSL
                     TDVTYVFDEPTAGLHPHDIQRMNRLLLRLRDKGNTVLVVEHKPQTIAIADHIVDLGPG
                     AGTAGGTVCFEGTLEGLRASNTLTGRHLDDRAALRASVRKPTGKLKIRGASSHNLKKV
                     NVDIPLGVLVVVTGVAGSGKSSLVHGSLPAGADVVSIDQSPVHGSRRSNPATYTGLLE
                     PIRKAFAKANGVKPALFSPNSEGACPTCNGAGVIYTDLGMMAGVAIPCESCEGKRFQA
                     EVLTYLLGGKNISEVLAMPVSGATKFFGAGPAKIPAAHAILARLADVGLGYLGLGQPL
                     TTLSGGERQRLKLATHMAEEGGTYVLDEPTTGLHLADVAQLLGLLDRLVDAGKSVIVI
                     EHHPAVMAHADWIIDLGPGAGHEGGRVVFEGTPAELVAARSTLTGEPTPTEGGGDTAT
                     QNGDADSGGDGGQDDGGGETISCTCRGDVEHTPWGGLVLSALGLAFIRRRRAA"
BASE COUNT          466 a         1056 c          938 g          367 t
ORIGIN      
        1 ccgagatgat ggccaagggg acctacggct ggatcctgct ggccacgccc gacctcgacg
       61 gcacgttcga gaagctgcag gccggggacg ccgaggtcgt gcaggagccg accgagcagc
      121 cctacggcgt ccgcgactgc gccgtacgcg accccgccgg caacctgatc cgcatccagg
      181 agcagcgccg gaccgcccgc cgctcgcagg cctgaccgac gagacacgcc atggccacca
      241 agaccgacac gaagtcgccc gagctgcacc ccgccgacag tcacgcccgc atccgggtgc
      301 acggcgcgcg cgtcaacaac ctcaaggaca tcagcctcga catcccgaag cgccggctga
      361 cggtgttcac cggcgtgtcg ggctcgggca agagctcgct ggtgttcggc acgatcgccg
      421 cggagtcgca gcgcctgatc aacgagacct acagcgcctt cgtgcagggc ttcatgccga
      481 cgctcgcgcg cccggacgtc gacgtgctcg acggcctgac gaccgcgatc ctcgtcgacc
      541 aggagcgcat gggcgccaac gcccgctcga ccgtcggcac cgcgaccgac gccaacgcga
      601 tgctgcggat cgtgttcagt cggctcggaa agccgcacat cggctcgccg caggcgttct
      661 ccttcaacgt cgcttccatc agtggcgctg gcgccgtcac gctggaacgt ggcggaaaga
      721 ccgtcaagga gcggcgcagc ttcagcatca ccggcggcat gtgcccgcgc tgcgagggcc
      781 gcggcgcggt caccgacatc gacgtgtcgg cgctgtacga cgacagcaag tcgctcaacg
      841 agggcgcgat cacgatcccc ggctacacga tggagggctg gtacggccgg atcttccgcg
      901 gctgcggcta cttcgacccg gacaagccga tccgcaaatt caataaaaag gagctgcacg
      961 acctgctcca ccgggagccg accaagatca agatcgacgg catcaacctg acctacgagg
     1021 gcctgatccc gaagatccag tcctcgttcc tggccaagga cgtcgacgcg atgcagccgc
     1081 acgtgcgcgc cttcgtcgag cgcgcggtca ccttcaccac ctgccccgag tgcgagggta
     1141 cccggctcag caaggaggcc cgctcgtcga agatcaaggg ggtcaacatc gccgacgtgt
     1201 gcgcgatgca gatcagcgac ctcgcggagt gggtccgcgg cctgcgggag ccctcggtcg
     1261 cgccgctcct cgccaacctt caacaggtgc tcgactcgtt cgtcgagatc ggcctcggat
     1321 acctctcgct cgagcggccc tcgggcaccc tgtcgggcgg cgaggcccag cgcgtcaaga
     1381 tgatccgcca cctcggatcg tcgctgaccg acgtcaccta cgtcttcgac gagccgaccg
     1441 ccggtctgca cccgcacgac atccagcgga tgaaccggct gctgctgcgc ctgcgcgaca
     1501 agggcaacac ggtgctcgtc gtcgagcaca agccgcagac gatcgcgatc gccgaccaca
     1561 tcgtcgacct cggccccggc gccggcacgg cgggcggcac cgtctgcttc gagggcaccc
     1621 tcgagggcct gcgcgccagc aacaccctca ccggccgcca cctcgacgac cgcgccgcgc
     1681 tccgggcgag cgttcgcaag ccgaccggca agctcaagat ccgcggcgcc agctcgcaca
     1741 acctcaaaaa ggtcaacgtc gacatcccgc tcggcgtgct cgtcgtcgtc accggcgtcg
     1801 ccggctcggg caagagctcg ctcgtgcacg gctcgctgcc ggccggcgcc gacgtcgtgt
     1861 cgatcgacca gagccccgtg cacggctcgc ggcgcagcaa ccctgccacc tacaccggcc
     1921 tgctcgagcc gatccgcaag gcttttgcca aggccaacgg cgtcaagccg gcgctgttca
     1981 gccccaactc cgagggcgcc tgtccgacct gcaacggcgc cggcgtgatc tacaccgacc
     2041 tcggcatgat ggccggcgtg gcgatcccct gcgagtcgtg cgagggcaag cgcttccagg
     2101 ccgaggtgct gacctacttg ctcggcggca agaacatcag tgaggtgctg gcgatgccgg
     2161 tcagcggggc gacgaagttc ttcggcgccg gtccggcgaa gatccccgcc gcgcacgcga
     2221 tcctcgcgcg cctcgccgat gtcgggctcg gctacctcgg gctcggccag ccgctgacga
     2281 cgctgtccgg cggcgagcgc cagcggctca agctggcgac gcacatggcc gaggagggag
     2341 gcacctacgt gctcgacgag ccgaccaccg gcctgcacct cgccgacgtc gcgcagctgc
     2401 tcggcctgct cgaccgcctc gtcgacgccg gcaagtcggt gatcgtgatc gagcaccacc
     2461 cggccgtgat ggcccacgcc gactggatca tcgacctcgg ccccggcgcc ggtcacgagg
     2521 gcggccgggt cgtcttcgag ggcacgcccg cggagctcgt cgccgcccgc tcgaccctca
     2581 ccggcgagcc gaccccgacc gagggcggcg gcgacacggc gacgcagaac ggcgacgcgg
     2641 acagcggcgg cgacggcggc caggacgacg gcggcggcga gacgatcagc tgcacgtgcc
     2701 gcggcgacgt cgagcatacc ccgtggggcg ggctggtgtt gagcgcgctc ggcctcgcgt
     2761 tcatccgccg gcgccgcgcg gcgtgaacgc ggcccgcgtg acctgtccca gggggcaggt
     2821 cacgcgc
//
LOCUS       DASWNJ010000685         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_153907, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000685 DASWNJ010000000
VERSION     DASWNJ010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153907"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..>2008
                     /locus_tag="VGB85_11450"
     CDS             217..>2008
                     /locus_tag="VGB85_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134693.1"
                     /translation="MELRETIDLEVERLGLPGLRTELAALEQEMFSLGRREAELRRTI
                     SLWDRWMIFHDTAAEKQVKGGRRRRAQVAKSLAYVKTQVAAANATLGEMCPPFAVAVG
                     LAECLPLAYTGLAVEGIIFRRVGRGDELREALAGLIGLLRRTYFPGLDFAALRARLAD
                     ASACQTLAGLAEVPLPMHTRLGHAPLEGDAPLGKVAERLLDNGFFAARYREDELARRC
                     ADLQSAATAANAAVGFWDRINVLSLSPDELVRDEARAAIKAAQEELRQIVDRQQRLLH
                     DAMRAYPPLDFYHGLVEALGIVELLKADREPVLSAKGTIERRAVIVPRALLLAAIRRL
                     QDSFAATFPGVPAPRELANRSAGESPRLRGYASATAFMERAAASKRLQALRDEALVHA
                     GMIGQLGRELAEVEGQISLVDRLVFWSDTDAEKAAKMLARRAEANVAWTWELWEKMLA
                     EARAIGAGIGPIVTRDWALVVIARIAAIHTDSGSSSFPKDCQVYKREEAIESINGLRG
                     VFHKIYGVVGGVSELMVAALDARPADPAVLEDPIHGYAPLGAAELHGLLARRLEGTAF
                     RGLYQEVSQRLGTLHQASEERASVEGEISMW"
BASE COUNT          260 a          626 c          826 g          296 t
ORIGIN      
        1 cggtcgcgcc gaccagcggc gacccgagcg acattccggc gttcctgcgc cggcgcgacg
       61 acagcggctt tacccgctga gtcggtgagc gtgaagacgc cgcgacgcct ccgggcgcgc
      121 ggcgtctggc aattgtgcgc gtgaacgagc gaggccgggc gatccgccgc acgggcggag
      181 ggccgcggtt cgggtcgctt gtggcacgct gcggtgatgg agctgcggga gacgatcgac
      241 ctcgaggtgg agcgcctcgg tttgccgggt ttgcggaccg agctggcggc gctcgagcag
      301 gagatgttct cgctggggcg gcgcgaggcg gagctgcggc ggacgatctc gctgtgggac
      361 cgctggatga tctttcatga cacggcggcc gagaagcagg tcaagggcgg gcggcggcgg
      421 cgggcgcagg tggcgaagtc gctggcgtac gtgaagacgc aggtcgccgc cgcgaacgcg
      481 acgctgggcg agatgtgtcc gccgttcgcg gtcgcggtcg ggctcgcgga gtgcttgccg
      541 ctggcgtaca ccgggctcgc ggtcgagggg atcatctttc gtcgcgtcgg tcgcggggac
      601 gagctgcgcg aggcgctggc cgggctgatc gggttgctgc ggcggaccta cttcccgggg
      661 ctcgactttg cggcgctgcg ggcccggctg gccgacgcct ccgcgtgtca gacgctcgcg
      721 gggctcgccg aggtgccgtt gccgatgcac acgcggctcg gccacgcgcc gctcgagggt
      781 gatgcgccgc tgggcaaggt tgcggagcgg ctgctcgaca acggcttctt cgcggcccgc
      841 tatcgtgagg acgagctggc gcgccgctgc gccgatctcc agagcgccgc gacggcggcg
      901 aacgccgcgg tcggcttctg ggatcggatc aacgtgctgt cgctgtcgcc cgacgagctg
      961 gtgcgcgacg aggcgagggc ggcgatcaag gcggcgcagg aggagctgcg gcagatcgtc
     1021 gatcgccagc agcggctgtt gcacgacgcg atgcgggcct acccgccgct cgacttttat
     1081 cacgggctcg tcgaggcgct cggcatcgtc gagctgctca aggccgaccg ggagccggtg
     1141 ctgtcggcga agggcacgat cgagaggcgg gcggtgatcg tgccgcgggc gctgctgctg
     1201 gcggcgatcc ggcggttgca ggactcattc gcggcgacct tccccggcgt gccggcgccg
     1261 cgcgagctgg ccaaccgcag cgcgggcgag tcgccgcgcc tgcgcgggta tgcgtcggcg
     1321 acggcgttca tggagcgcgc ggcggcctcg aagcggctgc aggcgctgcg cgacgaggcg
     1381 ctggtgcacg cgggtatgat cggccagctc gggcgcgagc tcgccgaggt cgagggccag
     1441 atctcgctcg tcgaccggct ggtgttctgg tcggacaccg acgccgagaa ggccgcgaag
     1501 atgctggccc ggcgggcgga ggccaacgtc gcgtggacct gggagctgtg ggagaagatg
     1561 ctcgccgagg cgcgggcgat cggcgcgggg atcgggccga tcgtcacgcg cgactgggcg
     1621 ctggtggtga tcgcccgcat cgcggcgatc cacaccgaca gcgggtcgag cagctttccc
     1681 aaggactgtc aggtgtacaa gcgcgaggag gcgatcgagt cgatcaacgg cctgcgcggc
     1741 gtctttcata agatctacgg ggtcgtcggc ggcgtgtcgg agctgatggt cgcggcgctc
     1801 gacgcccggc cggccgatcc cgcggtgctc gaggacccga tccatgggta cgcgccgctg
     1861 ggggcggccg agctgcacgg actactggcg cggcggctcg agggcacggc gtttcgtggg
     1921 ctgtatcagg aggtctcgca gcggctcggc acgctgcatc aggcgagcga ggagcgggcc
     1981 agcgtcgagg gcgagatctc gatgtggg
//
LOCUS       DASWNJ010000686         2255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_260755, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000686 DASWNJ010000000
VERSION     DASWNJ010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260755"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="VGB85_11455"
     CDS             <1..1265
                     /locus_tag="VGB85_11455"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY0134694.1"
                     /translation="DTPKQIDDAMCWGYGWQLGPFALMDAAGVAWCAEQLQGMGITPA
                     PALTQLIAEQGQGARWYAGSVSAPTVYVPGTGAKPIPTPVGVLVLAAEKDRRGELHSN
                     ATAGLIDLGDGIACLEFRGKMNVLDDGAVTMMQEANATLARIGGFRGLVIGNQGENFC
                     AGADLRTILKAAEAQDFATLERAVATLQNALMDLRHGDLPVVVAPHGMTLGGGVEVTL
                     HGDAVVASSELYMGLVEIGVGLLPAGGGLKEMCRRASEWAAQSPEPDPYPLIRRAFEN
                     AAAGKVSTSAHEARALGFLRADDSVVFHRARVLAEAKRRAIGLAEAGYVPPDRNAPIT
                     LVQPGRGASLLMGAQMFFWGGYASAHDQKIAQKIAHVLSGGMTGQTTATAQQLLDLER
                     EAFCSLAGEPATVARIKNMLDTGKPLRN"
     gene            1287..>2255
                     /locus_tag="VGB85_11460"
     CDS             1287..>2255
                     /locus_tag="VGB85_11460"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY0134695.1"
                     /translation="MTTLQDAVIVASRRSAVGRALKGTLRNTRPDDFAAQVIRGLLAD
                     LPGLPLDLIDDVALGCAMPEAEQGLNVARIIALRAGLPNTSSAQTINRFCASGLQAIA
                     TCAERIRVGAIEAAIAGGVESMSMVPMGGNKAVLNPTIVSEQPEIYISMGLTAENVAR
                     KFEVSRADQDAFALASHIKAVAAIKAGKFTGEIVPVTTAEGTTFAVDEGPRADTTQDA
                     LGQLRPVFHAQGSVTPGNSSPINDGAAASVVMSAARAEALGLRPLARFVGFAVAGVAP
                     EIMGIGPIAAIPKVLAQTGLTLADIDLIELNEAFASQSLAVIRKLGL"
BASE COUNT          336 a          888 c          733 g          298 t
ORIGIN      
        1 ccgacacccc gaagcagatc gacgacgcga tgtgctgggg ctacggctgg cagctcgggc
       61 cgttcgcgct catggacgcc gccggtgtcg cctggtgcgc cgagcagctc cagggcatgg
      121 gcatcacgcc cgcgccggcc ctcacgcagc tgatcgccga gcagggccag ggcgcgcgct
      181 ggtacgccgg ctccgtctcc gcgccgaccg tctacgtgcc cggcaccggc gcgaagccga
      241 tcccgacgcc cgtcggcgtg ctcgtgctcg ccgccgagaa ggaccgccgc ggcgagctgc
      301 acagcaacgc caccgccggc ctgatcgacc tcggcgacgg catcgcctgc ctcgagttcc
      361 gcggcaagat gaacgtgctc gacgacggcg cggtcacgat gatgcaggag gccaacgcga
      421 cgctcgcgcg catcggcggc tttcgtggcc tcgtcatcgg caatcagggc gaaaacttct
      481 gcgccggcgc cgacctgcgc acgatcctca aggccgccga ggcccaggac ttcgcgacgc
      541 tcgagcgcgc cgtcgccacc ctgcaaaacg ccctgatgga cctgcgccac ggtgacctcc
      601 cggtcgtcgt cgcgccgcac ggcatgaccc tcggcggcgg cgtcgaggtc accttgcacg
      661 gcgacgccgt cgtcgccagc agcgagctgt acatgggcct ggtcgagatc ggcgtcgggc
      721 tcctgccggc cggcggcggc ctcaaggaga tgtgccgccg cgcctccgag tgggccgcgc
      781 agtcacccga gcccgacccc tacccgctga tccgtcgcgc gttcgagaac gccgccgcgg
      841 gcaaggtcag cacctccgcc cacgaggcgc gcgcgctcgg ctttttgcgc gccgacgaca
      901 gcgtcgtgtt ccaccgcgcg cgcgtgctcg ccgaggccaa gcgccgcgcg atcggcctcg
      961 ccgaggccgg ctacgtcccg cccgatcgca acgccccgat caccctcgtg caacctggcc
     1021 gcggggccag cttgctcatg ggcgcgcaga tgttcttctg gggcggctac gcctccgccc
     1081 acgaccaaaa gatcgcccag aagatcgccc acgtgctgtc cggcggcatg accggccaga
     1141 ccaccgcgac cgcgcagcag ctcctcgacc tcgagcgcga ggcgttttgc agcctcgctg
     1201 gcgagccggc caccgtcgct cgcatcaaga acatgctcga caccggcaag cccctccgca
     1261 actaaccccg gaatcacgga ctcccaatga ccacgcttca agacgccgta atcgtcgcat
     1321 cccgccgcag cgcggtcggg cgcgccctca agggcacgct ccgcaacact cgccccgatg
     1381 acttcgccgc gcaggtcatc cgcgggctgc tcgccgacct ccccggtctg ccgctcgatc
     1441 tgatcgacga cgtcgcgctc ggctgcgcga tgccggaggc cgagcagggc ctcaacgtcg
     1501 cccgcatcat cgcgctgcgg gccggcctgc cgaacaccag ctccgcgcag acgatcaacc
     1561 gcttctgcgc ctcgggcctg caggcgatcg ccacctgcgc cgagcgcatc cgcgtcggcg
     1621 cgatcgaggc cgcgatcgcg ggcggcgtcg agtcgatgag catggtgccg atgggcggca
     1681 acaaggccgt gctcaacccg accatcgtct ccgagcagcc cgagatctac atctcgatgg
     1741 gcctgaccgc cgagaacgtc gcgcgcaagt tcgaggtctc gcgcgccgac caggacgcct
     1801 tcgccctcgc ctcgcacatc aaggccgtcg ccgcgatcaa ggccggcaag ttcacgggcg
     1861 agatcgtgcc agtgaccacc gccgagggca ccaccttcgc cgtcgacgag ggtccgcgcg
     1921 ccgacaccac gcaggacgct ctcgggcagc tgcgcccggt gttccacgcc cagggcagcg
     1981 tgaccccggg caactcgtcc ccgatcaacg acggggccgc ggcgagcgtc gtgatgtcgg
     2041 ccgcccgcgc cgaggcgctc ggcctgaggc cgctcgcgcg cttcgtcggc ttcgcggtcg
     2101 ccggggtcgc cccggagatc atgggcatcg gcccgatcgc cgcgatcccc aaggtcctcg
     2161 cgcagaccgg cctgacgctc gccgacatcg acctgatcga gctcaacgag gcgttcgcca
     2221 gccagtcgct cgccgtcatc cgcaagctcg gcctc
//
LOCUS       DASWNJ010000687         2378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_189490, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000687 DASWNJ010000000
VERSION     DASWNJ010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189490"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VGB85_11465"
     CDS             complement(<1..272)
                     /locus_tag="VGB85_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134696.1"
                     /translation="MRLAQDAGMTTSFTRALSLSVRLLAATCGAALASTVLLGVSGCE
                     TAICDDFGIELSFTHEDSTTTATLRAIAMETTGRAVAVGDGGVLLTR"
     gene            complement(448..990)
                     /locus_tag="VGB85_11470"
     CDS             complement(448..990)
                     /locus_tag="VGB85_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134697.1"
                     /translation="MMRSLGLLVVVSTLLAGCTPPLDEDQAAQLARALDTVNPEQQPV
                     VAARGLEELERGRLGDALADSLDALVSAAPDERSQKVAMGLQGSEARRGWSQVCRTDY
                     DEQVARFASAAPADGVRVLMEACDLGRIGLQPADPAKVDGMYLIVALIVYGAIERRGS
                     VHPVEKRALALMAARAPETP"
     gene            complement(1189..1929)
                     /locus_tag="VGB85_11475"
     CDS             complement(1189..1929)
                     /locus_tag="VGB85_11475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0134698.1"
                     /translation="MTRSTSMGDVDADANTSSISDTAGDASGGASMGEDAASTTDTST
                     GAGLTTGEPGPLCGDGVKEGEEECDDANQSEGDGCLSNCTREWFVFVTSEPFTQGDIT
                     GSVGADYQCRHRAAKMFLPNGERYMAWISTSEVQPVDRMYHARGPYKLVNGLRVAAGW
                     DALIAGPLENPIMVSELGETVDALVFTGTQPSGERVLDSTHCDDWSDSDGTNFGWYGV
                     SSEVGVAWTLAIEEFCGGNAAIYCFEQP"
     gene            complement(2046..>2378)
                     /locus_tag="VGB85_11480"
     CDS             complement(2046..>2378)
                     /locus_tag="VGB85_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134699.1"
                     /translation="PVDRMYHARGPYKLVLGLRVAASWDALIAGPLENPIMVSEFGET
                     VDAPVFTGTQPNGERMLDSTRCDDWTDSSGDNFTWIGVSSEVGAEWTLAAESFCGGPA
                     AIYCFEQP"
BASE COUNT          387 a          864 c          753 g          374 t
ORIGIN      
        1 cgggtcagca gcacgccgcc gtccccgacc gcgacggcgc gcccggtcgt ctccatcgcg
       61 atcgcgcgca gcgtcgcggt cgtcgtgctg tcctcgtgcg tgaaggacag ctcgatccca
      121 aagtcgtcgc agatcgccgt ctcgcagccg gagaccccca ataacacggt cgaggcgagg
      181 gccgccccgc aggtggcggc gagcaggcga acggagagcg agagggcacg cgtgaacgag
      241 gtcgtcatgc cggcgtcttg agcaaggcgc atgccgccga gttcgccaat gatctccgga
      301 gccatggcag gcgtgtcacg gacacggatg tcaggccgtc gtcgacatac ccgggaatgc
      361 ggccagcggc atgatcgatg tcggccgctc gcaagcgatg acggcgaggc tacccatgca
      421 gcgactgccg atcgaccggc gcgaagctca gggggtctcg ggggccctcg ccgccatcaa
      481 cgcgagcgct cgcttctcca ccgggtgcac gctgccccgg cgttcgatcg cgccgtacac
      541 gatcagggcg acgatgagat acatcccgtc gaccttggcc gggtcggcag gttgcaggcc
      601 gatccgcccg agatcacagg cttccatgag cacgcggacc ccgtccgccg gggcggccga
      661 cgcgaaccgg gccacctgct cgtcgtagtc cgtgcggcac acctgcgacc agccgcgcct
      721 cgcctcggac ccctggagcc ccatggcgac cttctggctc cgttcgtcag gtgcggcgct
      781 gacgagggcg tcgaggctgt ccgccagcgc gtcgccgagc cggccgcgct cgagctcctc
      841 gagaccgcgt gcggcgacca ccggctgctg ctccgggttg acggtgtcga gcgcgcgagc
      901 cagctgcgcg gcctgatcct cgtcgagcgg cggggtacag ccggcgagca gagtcgatac
      961 gacgacgagc aaccccagcg agcgcatcat cacgcgacgg tcccatcgcc ggcccacccc
     1021 gtcaatctct cgcggcgcgc cagcacgtgc atacgtggca cccaccacag gtacacccgg
     1081 cgagcagaat tggcgcgggc ggacgcgccc gtgcgaggag gcgcaggcct ccgcccgggc
     1141 gcgcatgcag acctcggagg tgtgatgacg ccccgtgaac tggcgaaatc acggctgctc
     1201 gaagcaatag atcgccgcat ttcctccgca gaattcctcg atcgccagag tccatgcgac
     1261 gccgacctcc gaactgacgc cataccatcc gaagttggtg ccatcgctgt cgctccaatc
     1321 gtcgcaatga gtcgagtcga gcacccgctc cccgctcggc tgagtccctg tgaacaccag
     1381 ggcatcgacg gtctccccga gctcgctgac catgatcgga ttctccagcg gccctgcgat
     1441 caatgcgtcc cagcccgccg cgacacgcag accgttgacg agcttgtacg gaccacgggc
     1501 gtgatacatg cggtcgaccg gctggacctc gctcgtgctg atccacgcca tataacgctc
     1561 cccattgggc agaaacatct tggccgcgcg gtgccggcac tggtagtccg cgccgacgct
     1621 ccccgtgatg tcgccctgcg tgaacggctc gctggtcacg aacacgaacc attcacgcgt
     1681 gcaattgctc aggcaaccat caccctcgct ctggttcgcg tcgtcacact cctcctcgcc
     1741 ctccttcaca ccgtcgccgc acaacggccc cggctccccg gtcgtcagcc ccgcgccagt
     1801 gctcgtgtcg gtcgtgctcg cggcgtcttc tcccatcgag gccccaccgc tggcgtcgcc
     1861 cgccgtatcg ctgatgctcg aggtgttcgc gtcggcgtcg acatctccca tgctcgtcga
     1921 cctggtcata tcggtggacc cgtgcatccc ttcgctcacg tcccctgagc aggccggtgc
     1981 gagcaggagc agcaagacag gcgctacacg atggtcaccg ggctgcatga tggctttcgt
     2041 cgccgtcacg gctgctcgaa gcaatatatc gccgcggggc cgccgcagaa gctctcggct
     2101 gccagggtcc attcggcgcc gacctccgag ctcacgccga tccaagtgaa attatcgccg
     2161 ctgctgtcgg tccagtcgtc gcagcgcgtc gagtccagca tgcgttcgcc gttcggctgg
     2221 gtcccggtaa agaccggcgc gtcgacggtc tcgccgaact cgctgaccat gatcggattc
     2281 tccagcggac ctgcgatcaa ggcgtcccag ctggccgcga cacgcagacc gaggaccagc
     2341 ttgtacggac cacgggcgtg atacatgcgg tcgaccgg
//
LOCUS       DASWNJ010000688         1636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_296031, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000688 DASWNJ010000000
VERSION     DASWNJ010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296031"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VGB85_11485"
     CDS             complement(<1..782)
                     /locus_tag="VGB85_11485"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY0134700.1"
                     /translation="MAAVGLSVSCRKAAPEPEVPAEGGAVASEDAARLPAFDMSDDSP
                     AGDGVPFERFFVELGDAPVRGSSAAPVTIVAFSDFECPYCEKGHETILQVEREYGEKV
                     RIAYKAYPLDFHSHAMIAALAARSAQDQGKFWQFHDLLFSQRGLDMPTLDAYARQAGL
                     DMDRLHADLEALKWGPSVSRDMRQARKLGVTGTPAYFVNGRFLSGAKPIEVMRALIDQ
                     EIDLAVKWQAQGVAPDKVYEHAIAKGYREVQYAKPRKGLKPDV"
     gene            975..1463
                     /locus_tag="VGB85_11490"
     CDS             975..1463
                     /locus_tag="VGB85_11490"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY0134701.1"
                     /translation="MQRQREMTMRFLAEPSAVNFGGKVHGGSVMTWIDQAGYACAVGW
                     SGRYCVTRYVGGIRFQRPIVIGHVVEVRARVIYTGTTSMHIAVDVSSADPRDGVYSQT
                     THCVVVFVAVDDAGKPAAVTPWEPQTDDDRAQVAYALRLMELRKGIEQEMDAASHRAD
                     AS"
BASE COUNT          247 a          631 c          526 g          232 t
ORIGIN      
        1 acatccggct tgagcccctt gcgcggcttg gcgtactgca cctcgcggta gccctttgcg
       61 atcgcgtgct cgtacacctt gtcgggcgcg accccctggg cctgccactt caccgcgaga
      121 tcgatctcct ggtcgatgag cgcgcgcatc acctcgatcg gcttggcccc gctgaggaac
      181 cggccgttca cgaaatacgc cggcgtgccc gtcaccccga gcttgcgggc ctgtcgcatg
      241 tcacgcgaga cgctcggccc ccacttgagc gcctcgaggt cggcgtgcag gcggtccatg
      301 tcgaggccgg cctggcgcgc gtacgcgtcg agcgtcggca tgtcgagccc gcgctgactg
      361 aacagcaggt cgtggaactg ccagaacttg ccctggtcct gcgccgagcg ggccgcgagc
      421 gcggcgatca tcgcgtggct gtggaagtcg agcgggtacg ccttgtacgc gatgcggacc
      481 ttctcgccgt actcgcgctc gacctgaagg atcgtctcgt ggcccttttc gcagtacggg
      541 cactcgaagt ccgagaacgc cacgatcgtc accggcgccg ccgacgagcc gcgcaccggc
      601 gcgtcgccca gctcgacgaa gaagcgctcg aacggcaccc cgtcgcccgc cggcgagtcg
      661 tcgctcatgt cgaacgccgg caacctcgcc gcatcctcgg aggccaccgc cccgccctcc
      721 gcgggcacct ccggctccgg cgccgccttg cgacacgaga ccgacaggcc cacagcggcc
      781 accacagcgc agatccaggg gagacgagcg aacgacgggc gcagcatggt cgaccgcatt
      841 gtacgcccca ccgcccccga acgatctcac ccgcgaggtg cgcttgccca tacccgcgcg
      901 cggccgcagc gcccgcgctg gacacgcccg ccgccggttt gacgcccact tgcgccgggc
      961 agtaccgtcg cccgatgcag cgccagcgcg agatgacgat gcgcttcctc gccgagccct
     1021 cggcggtcaa cttcggcggc aaggtccacg gcggctccgt gatgacgtgg atcgaccagg
     1081 ccggttatgc ctgcgcggtc ggctggagcg ggcgctactg cgtgacccgc tatgtcggcg
     1141 ggatccggtt ccagcgcccg atcgtcatcg gccacgtcgt cgaggtgcgc gcccgcgtga
     1201 tctacaccgg caccaccagc atgcacatcg ccgtcgatgt ctcctcggcc gacccgcgcg
     1261 acggcgtcta ttcgcagacc acccactgcg tcgtcgtctt cgtcgccgtc gacgacgccg
     1321 gcaagccggc cgccgtcacg ccctgggagc cgcagaccga cgacgaccgc gcccaggtcg
     1381 cctacgccct gcgcctgatg gagctgcgca agggcatcga gcaggagatg gacgccgcca
     1441 gccaccgcgc cgacgcctcc tgaccccgcg cgcacactcg cccacggacc gccgggaagc
     1501 cggccgacac tttcgcggta ccatcgccga cccgcgggaa cttcgacccc cggcgaggcg
     1561 tcctgacgac gcggaggcag cgcatgcagc aggtccagtg cgacgatgtc catcactctt
     1621 acgacggcgt acgcga
//
LOCUS       DASWNJ010000689         1963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_471689, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000689 DASWNJ010000000
VERSION     DASWNJ010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471689"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VGB85_11495"
     CDS             complement(<1..264)
                     /locus_tag="VGB85_11495"
                     /inference="COORDINATES: protein motif:HMM:NF015699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEY0134702.1"
                     /translation="MPLRSMTGFGASTRVIELGGAPHVLGVELRAVNQRFLEIKLRQP
                     FGAAAEHELRRAVEARLGRGRVDLMVHLQAAREGAAREGAAREG"
     gene            360..1052
                     /locus_tag="VGB85_11500"
     CDS             360..1052
                     /locus_tag="VGB85_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134703.1"
                     /translation="MNRLVRMLAGPAVGFGLLAALAAGLAPTVAAAGKPPRERKFLLG
                     VEGMGLQVPALRPKIAALDSKYLGPTVTLGGIGVIGRWQVVPIVALELAVRTGSLRYH
                     SRGDVISQDMVLAEAGALLYLLRNTIGHLAIDGGAGGLVHAIRYELHDRPRGSQRVGA
                     ALIHVGVDLEIRLRRIAFTASLRSYGVITDVARTAARGALFDGVPESQRRAPVAVLST
                     YLLGSVGIAYRF"
     gene            complement(1059..1223)
                     /locus_tag="VGB85_11505"
     CDS             complement(1059..1223)
                     /locus_tag="VGB85_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134704.1"
                     /translation="MATWIFILHMVFPIHSDLPAPGVVTPTADATDEVVRYRGLEQMF
                     ARFEAEAEPA"
     gene            complement(1331..>1963)
                     /locus_tag="VGB85_11510"
     CDS             complement(1331..>1963)
                     /locus_tag="VGB85_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134705.1"
                     /translation="RSAADLALTELGWIDTHGQRRRGTSPLRVPITWDGSPGLATGTV
                     RALRAAWKQIGIAAPSVTAGWAYVLSLLRGGKFNVALARLAGSSDMDLSPWFHSRGEH
                     NLTGVADHELDAALEHYRQAGSRAARDAAKQAIATRLAALHPVSVLYAPLQVLLASRG
                     LTGLEFIDDLPRLDSLGLGAQPGPGGLILPPPPAPPTDTPPTTPRHGDGG"
BASE COUNT          259 a          685 c          742 g          277 t
ORIGIN      
        1 accctcgcgc gcagcaccct cccgcgcagc accctcccgc gcagcttgga gatggaccat
       61 caggtcgacg cggccgcgac cgaggcgcgc ctcgacggcg cggcgcagct cgtgctccgc
      121 ggcggcgccg aacggctggc gcagcttgat ctcgaggaag cgctggttga ccgcccgcag
      181 ctcgacgccg aggacgtgcg gcgctccgcc gagctcgatg acacgggtcg aggcgccgaa
      241 accagtcatg cttcgcaggg gcatccatgc ttgctagccc gcatgacccc gcggccgcaa
      301 gcgccatgca gggccaccgg gccggggccg ccgcgctcat gatgatatgc tgcgggctcg
      361 tgaaccgcct cgttcgtatg ctcgccgggc cggccgtcgg ttttggcctg ctggccgcgc
      421 tcgcggcggg gctcgccccg actgtggccg ccgccggaaa gccgccgcgc gagcgaaaat
      481 tcctgctcgg ggtcgagggc atgggcctgc aagtgccggc cctgcgcccg aagatcgccg
      541 cgctcgacag caaatatctc ggaccgacgg tcaccctcgg cgggatcggc gtgatcgggc
      601 gctggcaggt ggtgccgatc gtcgccctcg agctcgcggt gcgcacgggc tcgctgcgct
      661 accacagccg cggcgacgtg atctcgcagg acatggtgct cgcggaggcc ggcgccctgc
      721 tctacctgct gcgcaacacg atcgggcacc tggcgatcga cggcggcgcc ggcgggctgg
      781 tccacgcgat ccgctacgag ctgcacgacc ggccgcgggg atcgcagcgg gtcggtgcgg
      841 cgctgatcca cgtcggcgtc gacctcgaga tccgtctgcg ccggatcgcc ttcacggcct
      901 cgctgcgcag ctacggggtg atcaccgatg tcgcccgcac ggccgctcgg ggggccctgt
      961 tcgacggcgt cccggagtcg cagcggcgcg ccccggtcgc ggtgctctcg acctacctgc
     1021 tcggctcggt cgggatcgcc taccgctttt agggtcgatc aggcgggctc ggcctcggcc
     1081 tcgaagcgcg cgaacatctg ctcgagaccg cggtaacgga cgacctcgtc ggtcgcgtcg
     1141 gcggtggggg tgacgacgcc gggggccggc aggtcgctgt ggatcgggaa aaccatgtgg
     1201 aggatgaaga tccaggtcgc catcgtgtgc tcctcgaaaa ccggggtaca cggggcttgt
     1261 agcagcggtg cctacatgct cccaaaaaac tggatcatcg cgacgcggtg tgccgaccgc
     1321 gaaaactgga tcagccgccg tcgccgtgcc gcggggtcgt gggcggtgtg tcggtgggtg
     1381 gggccggcgg cgggggcagg atcaggccgc caggacccgg ctgggcgccg aggccgaggc
     1441 tgtcgaggcg cggcaggtcg tcgatgaact cgaggccggt caacccgcgc gaggccagca
     1501 gcacctgcaa cggggcgtac agcacgctga ccgggtgcag cgcggcgagg cgggtggcga
     1561 tcgcctgctt ggccgcgtca cgggccgcgc ggctgccggc ctggcggtag tgctcgagcg
     1621 ccgcgtcgag ctcgtggtcg gccacgccgg tcaggttgtg ctcgccgcgg ctgtgaaacc
     1681 atggggagag atccatgtcg ctgctgccgg ccaggcgcgc gagggcgacg ttgaacttgc
     1741 cgccgcgcag cagactgagc acgtaggccc agccggccgt gacgctcggc gccgcgatcc
     1801 cgatctgctt ccaggcggcc cgcagcgccc gtacggtgcc ggtggcgagc ccggggctgc
     1861 cgtcccaggt gatcggcacc cgcagcggcg aggtcccgcg ccggcgctgg ccgtgggtgt
     1921 cgatccagcc gagctcggtc agcgccaggt cggccgcgct gcg
//
LOCUS       DASWNJ010000690         2504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_189511, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000690 DASWNJ010000000
VERSION     DASWNJ010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189511"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VGB85_11515"
     CDS             complement(<1..951)
                     /locus_tag="VGB85_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134706.1"
                     /translation="MQVYSGALYFRYTGAMRGRLRLFALSLALGACATGRQAHERLVE
                     ATPYATAPLPPRAKIFLVAGGIEVANFAAEVVAQRRLWLARGYAPDEIACYWAHPFPA
                     ALRADRAQYQALADELRPCYRASAELLRAHLLQASAAGLPFLYLYVTSHGNVDIMPEG
                     VPEDRLLPAEQALFDQYVIQLGGGIGAGVDPGTLALAIRRGADPAGLVFSPRLLRDVL
                     RAFPADTPKIVVLQACHSGGFLDEARPERRAETIASVPRLTAIAAARFDRTSFGCDSG
                     AEMTYFGEVYTRLLATRLVSDPTAIDWGALYRALFADIARI"
     gene            1108..1458
                     /locus_tag="VGB85_11520"
     CDS             1108..1458
                     /locus_tag="VGB85_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134707.1"
                     /translation="MRITSACTLALVAVFTLACAPRPAEQPAPSPSPAPATTATTAAP
                     GTAGDAGGASECAVDADCVPAGCCHPSTCAPASAKPDCAGVMCTMNCQPGTLDCGGRC
                     ACEAGSCKAILGQP"
     gene            1540..2127
                     /locus_tag="VGB85_11525"
     CDS             1540..2127
                     /locus_tag="VGB85_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134708.1"
                     /translation="MTDAHSFFGSQPTFHVDGREDPALAAQLIGLILSETLGEPPRCE
                     ATFHNWVEGALVYPDDRTLALTRPLEIRLSGELLFAGQIVTLEGRFPAARPPELRAIA
                     VGPVGAHDAHAGKLQLTHGAALRELYVCVERDASQPTARGVAVPTSGLRVGVHIELEG
                     VGTSFGGEFLVVETRHLFDQSHGLRTEFVATRGGR"
     gene            complement(2169..>2504)
                     /locus_tag="VGB85_11530"
     CDS             complement(2169..>2504)
                     /locus_tag="VGB85_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134709.1"
                     /translation="ATMGTATATATMGTATATMGSATMDTATATMGTETSPTTTAGTM
                     TATATATAGETEGTGGTGTATATGTATATAGTDTTDTAGTGGTGGTGGTDTAGTGGTD
                     TAGTGGTGP"
BASE COUNT          314 a          914 c          932 g          344 t
ORIGIN      
        1 gatccgggcg atgtcggcga acagcgcccg gtacagcgcg ccccagtcga tcgcggtcgg
       61 gtcgctcacc agccgcgtcg cgagcaggcg cgtgtacacc tcgccgaagt aggtcatctc
      121 ggcgcccgag tcgcagccga agctggtgcg gtcgaagcga gccgcggcga tcgccgtcag
      181 ccgcggcacg ctcgctatcg tctcggcgcg ccgctccggc cgggcctcgt cgaggaagcc
      241 gccgctgtga cacgcctgca gcacgacgat cttcggggtg tcggcaggga acgcgcgcag
      301 gacatcacgc agcagccgcg gcgagaacac caggcccgcc gggtcggccc cgcggcggat
      361 cgccagcgcg agcgtgcccg ggtcgacgcc ggccccgatg ccaccgccga gctggatgac
      421 gtactgatcg aagagggcct gctcggccgg cagcaacctg tcctcgggga caccctcggg
      481 catgatgtcg acgttgccgt ggctggtgac gtagaggtag agaaagggca gcccggccgc
      541 gctggcttgc agcaggtggg cgcgcagcag ctcggcggag gcgcggtaac acggccgcag
      601 ctcgtcggcg agcgcctggt actgggcccg gtcggcgcgc agggccgcgg ggaacgggtg
      661 cgcccagtaa cacgcgatct cgtcgggggc gtagccgcgg gcgagccaca ggcgccgctg
      721 cgcgacgacc tcggcggcga agttcgcgac ctcgatcccg ccggccacga ggaagatctt
      781 ggcgcgtggc ggcagcgggg cggtcgcgta gggcgtcgcc tcgaccagcc gctcgtgcgc
      841 ctgccggccc gtcgcgcagg cgccgagcgc gagcgacagg gcgaacagac gcaggcgacc
      901 ccgcatcgcc ccggtatagc ggaagtagag cgcgccggaa tacacttgca tcggctgggc
      961 gccaggatca ggtcgcctgc ggatcgccgc ccgcgctcgc caggagcccc aggacggcga
     1021 acgcgcgcat tggtagatcc tacccttccc ttgagtggag aaccggcagg acccccgttc
     1081 aaaccatgcg tcgcgtataa gctccgcatg cgcatcacct ccgcttgcac cctcgccctc
     1141 gtcgccgtgt tcacgctggc ctgcgcgccg cgacccgccg agcagccggc cccgtcgccg
     1201 agcccggccc cggcgaccac cgcgaccacc gccgcgccgg gcaccgcggg cgatgcgggt
     1261 ggtgcgtccg agtgtgctgt cgacgccgac tgcgtgcccg cgggctgctg tcacccgagc
     1321 acctgcgcgc cggcgagcgc gaagccggac tgcgccgggg tgatgtgcac gatgaactgc
     1381 cagccgggca cgctcgactg cgggggccgc tgcgcgtgcg aggcgggcag ctgcaaggcg
     1441 atcctcggcc agccctgagc gcggtcgagc gggtgacgtc cgccccggtt caccagtaag
     1501 aaccccggcg ccgctcctgt cgtcctatca tcgcggggca tgacggacgc ccacagcttc
     1561 ttcgggagtc agcccacctt tcacgtcgac gggcgcgagg atccggcgct cgcggcccag
     1621 ttgatcgggc tgatcctcag cgagacgctc ggcgagccgc cgcgctgcga ggcgactttt
     1681 cataattggg tcgagggggc gctcgtgtat cccgacgacc gcaccctcgc gctcacgcgt
     1741 ccgctcgaga tccggctgtc cggggagctc ctgttcgccg ggcagatcgt cacgctcgag
     1801 gggcgcttcc ccgcggcccg gcccccggag ctgcgggcga tcgccgtcgg tccggtcggg
     1861 gcgcacgatg cccacgcggg gaagttgcag ctgacccacg gcgccgcgct gcgcgagctc
     1921 tacgtctgcg tcgagcgcga cgcgtcgcag ccgacggcgc gcggcgtcgc ggtgccgacc
     1981 agcggcctgc gggtcggcgt gcacatcgag ctcgagggcg tcgggaccag cttcggcggc
     2041 gagttcctcg tcgtcgagac caggcacctg ttcgaccagt cgcacggcct gcgcaccgaa
     2101 ttcgtggcga cccgcggcgg ccgatgatcg gctgagccag ggtcgccgcg gcgtcgggtt
     2161 aagctcgctc aggggcccgt gccgccggtg cccgcggtgt ccgtgccacc ggtgcccgcg
     2221 gtgtccgtgc cgccggtccc gccggtgcca cccgtgcccg cggtgtccgt ggtgtccgtg
     2281 cccgcggtgg cggtcgcggt gcccgtcgcg gtggcggtgc ccgttccgcc ggtaccctcg
     2341 gtctcacccg ccgtcgcggt ggccgtcgcc gtcatggtgc cggccgtggt ggtgggcgag
     2401 gtctcggtgc ccatcgtcgc ggtggcggtg tccatcgtcg ccgagcccat cgtcgcggtc
     2461 gccgtgccca tcgtcgcggt ggcggtggcg gtgcccatcg tcgc
//
LOCUS       DASWNJ010000691         7167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_118482, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000691 DASWNJ010000000
VERSION     DASWNJ010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7167
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118482"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /gene="tuf"
                     /locus_tag="VGB85_11535"
     CDS             <1..211
                     /gene="tuf"
                     /locus_tag="VGB85_11535"
                     /inference="COORDINATES: protein motif:HMM:NF015125.4"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEY0134710.1"
                     /translation="GTGYSPQFFFGACDATAVLDVGEVGTVTPGDRATVQFKLGKPIA
                     FEPGMKFAIREGGKTVGAGVVLETT"
     gene            complement(468..1304)
                     /locus_tag="VGB85_11540"
     CDS             complement(468..1304)
                     /locus_tag="VGB85_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134711.1"
                     /translation="MREHAGVHADDAVQGAEIGAPVDVPQRDASRLQAQRQDLAGGVV
                     GGRAGRFDVPARRDVADLHVGAVGPLVDRIEPNDLEVLGAATGRVVLRGDGDLGRHRR
                     ERDRGHLLGQRRRLRRVPPRLPLRARAQREATVPAGDHEVAGGAPRGRRHHRLAGRLE
                     LLVRLTPQVEHPARPVGVAGEHPPAVGRDRDTGDHARLVEVPVVAVVVVARGHEVRTM
                     HSPLPTVPIELADDQRAVELPDPQASRQRHAGANVGHHDALDRLTGGEGKAAQLRRPG
                     RP"
     gene            1435..2691
                     /locus_tag="VGB85_11545"
     CDS             1435..2691
                     /locus_tag="VGB85_11545"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0134712.1"
                     /translation="MQSGPGGLWFAELDAPAGELRYQVVGHVLGHSTNGTRADAFEPD
                     DAGDYTSVLRTTGGKVRIELDPAAFAPPGRSPDVVFADRDSPTARVAALALRRDALDH
                     EAIVTLITTMLADKTPDPNARPTGWPAFQAELAALADSDAPTPVRRAALALWFSFKPL
                     AQTPESAALARRALTELGATDPLWTLDPDALPNAIAATGATQEFVALSDAAIDGHPDP
                     ELAASLLLARLGEVAETGDEMAIRREYTRMQAPRFAGTGPRDLAGQYDPDRPLRAGRP
                     MPALDLELLDPPAGAPTKLDLAALRGHPVLIDIWATWCTPCIAAMPELHRLHKLRPQG
                     LEIVSIATDFDVEAVRAFRREKHKMPWRNVVVPEATSPDFHKRLGIVGIPLAVLLDEQ
                     GTIIASSPDLEVAQIPALLAKRAPAP"
     gene            complement(2706..4664)
                     /locus_tag="VGB85_11550"
     CDS             complement(2706..4664)
                     /locus_tag="VGB85_11550"
                     /inference="COORDINATES: protein motif:HMM:NF023149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HEY0134713.1"
                     /translation="MRTRIDLVPCALAFLIACQGDGGSSTDAASTGPGTTGTSATPTT
                     GDDPDPTTGSGPSTSTSTGTGTGTGGMDSSGAPDPTTGGGDGSSSGGESETGDVALGP
                     PVVRINIGGPAVEDFLADDGPESPYRASPDSQIFTTQDVAPDASVPAELPLAVLATGR
                     VEPDELSGGTGQLRWSIPVEPGEYQLRLHFAGPSQGPGSRVVDVRVDDVVALAALDIS
                     ALTAGDAGATVTLAVTADEWLDLELVRVPGSAMPLLAALELFGEGGLRDAPAGMVRHI
                     TVAGGGSGASLADAAGIGQLGALVGASAPGDEVWIHAGPEDYAIGGEISISAGGEAGA
                     PVVIRGVGADWHDAGRPRLVGTRADPWVADGAEGGVVFRLGAGADHLRFVNLGFANQG
                     NGCWRVTKPVAGLQLENIVADNVHRLLEDFVSGEGEAAITGLRLKDVSVRGYARAVAR
                     LQYDTSDVLFEDVFGDSEAQQYESFSTGITLAGPAHDVVFRRAVMLNNRQLDVEGYWN
                     ADGFSTERDNHAIVFEDTFAAGNTDGGYDLKSTDTVMTRATAVDNKRNFRLWGEISMN
                     ACVGLDPFKRGGSGTQAQLHINPQAQVKANDCRFIDDLPNTIVFDADDDGAAVVVGGC
                     VELNAAATYQTVEPAASLMLMDVADGCG"
     gene            complement(4797..6326)
                     /locus_tag="VGB85_11555"
     CDS             complement(4797..6326)
                     /locus_tag="VGB85_11555"
                     /inference="COORDINATES: protein motif:HMM:NF017396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070008 - serine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S28 family serine protease"
                     /protein_id="HEY0134714.1"
                     /translation="MSATLRLVVCAALLTACNLIQGTGSDTSAHDTGATSTGSTGTTD
                     DAVPTSGDASTAFGSDTASEPTSGGTSTDTTSEPTTGEPVDILERLLEIDGLEVEERE
                     SPVAGYRYFVMTFMQPADHADPDGLKFAQRLVLLHRDSSAPLVLATAGYFIDPDKPGL
                     GELATLLAANQLLVEHRFFLPSRPEPADWATLTIEQAAADLHAITGAFKPVYPAKWIS
                     TGASKGGMTSVYFRRFYPEDVDGTVAYVAPQSYGSSDPRYLDFVAGLGPADCRQALAD
                     VQRELLLRRPAMITRMQAQAVDGYTYDLLGLDRALETAVVELPFTFWQYGDEVDCLAV
                     PGPGASDDELWTFISDYNSPAYWSDYQTEMFEPYFWQAAVQLGYPALDESNVADLLLY
                     PGGDVAASYVLPGKVPVFDAAAMPDIADWLTAEGRAMLFIYGENDPYTAAAFEIEGAQ
                     DTYRFIAPGENHGARILDLVPAERDQALAALADWSGVTPKIPRSRPPAQDRHDLRRQP
                     R"
     gene            complement(6430..7077)
                     /locus_tag="VGB85_11560"
     CDS             complement(6430..7077)
                     /locus_tag="VGB85_11560"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0134715.1"
                     /translation="MSDSYDAFIRRAVPRYEEMSARLVDYLPPEASRILELGCGTGNL
                     SLRLAARYPAARLTLVDASPEMIAATRERMASAHPDVAAEYHTERFEALDLPPGGFDL
                     VTSSISLHHVLDKAPLYARIFAALAPGGSLRFADQLGGSARNHGHNWRRWLEHCREPG
                     NCDEAEVESLLDHAAAHDHYTPLAEHFAMLSACGFVDLDCVWRNWIWGIVTAERA"
BASE COUNT          994 a         2582 c         2580 g         1011 t
ORIGIN      
        1 cggcaccggc tactcgccgc agttcttctt cggggcctgc gacgcgacgg cggtgctcga
       61 cgtcggtgag gtcggcacgg tgacccccgg tgaccgggcc acggtccagt tcaagctcgg
      121 caagccgatc gccttcgagc cgggcatgaa gttcgcgatc cgcgagggcg gcaagacggt
      181 cggcgcgggc gtcgtgctcg agacgacctg acaaacgacg agggctccgc ccgccgacac
      241 accggcgggc ggagcctttg tattctttaa gaaaagagac ctgctatcct cgaccgatgt
      301 cgtcgcggct cgccgtcgct cagcaatgcg tatggagtga cgacggcaca tcgctggcgc
      361 tcgccaccga cacgggcgcc atcgtctggc atctcggagc cgacctcgcg ttgcaggtcg
      421 tcggcccggc ggagcgcctc cacgcggtcg cgttcgcggg cgacacgtta tggacggccg
      481 ggtcgacgga gctgcgccgc ttttccctcg ccaccggtga gccggtcaag cgcgtcgtga
      541 tggccgacgt tcgcaccggc gtgtcgctgg cgggacgcct gtgggtcggg caattcaaca
      601 gcacgctgat cgtcggcgag ctcgatggga acggtgggga gcggcgaatg catcgtccgg
      661 acctcatggc cgcgcgcgac gacgacgacc gcgacgactg ggacttcgac gagacgcgcg
      721 tggtcacctg tatcgcggtc gcgcccgacg gccggcggat gctcgccggc tacgccgacg
      781 gggcgtgctg gatgttcgac ctgcggggtg aggcgcacca gcagctccag gcgcccggcg
      841 agccgatggt gtcggcggcc gcgtggagcc ccgccggcga cctcgtggtc gccggcgggg
      901 acggtcgctt cgcgctgcgc acgggcccgc agtggcagcc gcggcggtac ccgccggagg
      961 cgacggcgct gtccgagcag gtgaccgcga tcgcgttctc gccggtgacg gccgagatcg
     1021 ccgtcacctc gcaatacgac tcgcccggtg gcggctccga ggacctcgag atcattcggc
     1081 tcgatacgat cgacgagcgg gccaaccgca cccacgtgca gatccgcgac atcccgacgc
     1141 gccggcacat caaatcggcc cgcgcggccg cctacgaccc ctccggcaag gtcctggcgc
     1201 tgtgcctgca ggcgggaggc gtcgcgctgt gggacgtcga caggcgcgcc tatctcggca
     1261 ccctgcactg cgtcgtcggc atggacgccg gcatgctcac gcatgcgccg cggaccgtcg
     1321 gcgaggcggg gcgcatcgag ctggtcggcg acacggccgc gctgacgcgc ctcggggacg
     1381 acctgtacgc gccgtggacc gacagctgcg tggtcaccag cggccgaggt gccgatgcag
     1441 tccggccccg ggggcctgtg gttcgcggag ctcgacgcgc cggcgggcga gctgcgctat
     1501 caggtcgtcg gccacgtcct cgggcactcg accaacggca cccgcgccga cgcgttcgag
     1561 cccgacgacg cgggcgacta cacctcggtg ctgcgcacca ccggcggcaa ggtccgcatc
     1621 gagctcgacc cggccgcgtt tgcgccgccc ggtcgctcgc ccgacgtcgt gttcgccgac
     1681 cgcgacagtc cgaccgcccg cgtcgccgcg ctcgcgctgc gccgcgacgc gctcgaccac
     1741 gaggcgatcg tcacgctgat cacgacgatg ctcgccgaca agaccccgga cccgaacgcg
     1801 cgccccaccg gatggcccgc cttccaggcc gagctcgccg cgctcgccga cagcgacgcg
     1861 ccgacccccg tgcgtcgcgc cgcgctcgcg ctgtggttta gcttcaagcc gctggcgcag
     1921 acccccgagt ccgcggcgct ggcccgccgg gcgctgaccg agctcggcgc gaccgacccg
     1981 ctgtggacgc tcgacccgga cgcgctgccg aacgcgatcg cggcgaccgg cgccacgcag
     2041 gaattcgtcg cgctcagcga cgcggcgatc gacggccacc ccgacccgga gctcgccgcc
     2101 tcgctgttgc tggcccggct cggcgaggtg gccgagaccg gcgacgagat ggcgattcgc
     2161 cgcgaataca cccgcatgca ggcgccgcgc ttcgcgggca cgggtccgcg ggacctcgcc
     2221 gggcaatacg accccgatcg tccgctgcgc gcgggccggc cgatgccggc gctcgacctc
     2281 gagctgctcg acccaccggc cggcgccccg acgaagctcg acctcgcggc cctgcgcggt
     2341 cacccggtat tgatcgacat ctgggcgacg tggtgcacac cgtgcatcgc cgcgatgccc
     2401 gagctgcacc gcctccacaa gctgcgcccg cagggcctcg agatcgtcag catcgccacc
     2461 gacttcgacg tcgaggccgt gcgcgcgttt cgccgcgaaa aacacaagat gccgtggcgc
     2521 aacgtcgtcg tgcccgaggc cacctcgccg gacttccaca agcggctcgg catcgtcggc
     2581 atcccgctgg cggtattgct cgacgagcag ggcacgatca tcgccagctc gcccgacctc
     2641 gaggtcgcgc agatccccgc gttgctggcg aagcgggccc ccgccccctg aggcgccggc
     2701 ctcgctcagc cgcagccgtc ggcgacgtcc atgagcatca agctcgcggc cggctcgacc
     2761 gtctgatacg tggccgcggc gttgagctcg acgcagccgc cgacgaccac ggcggcgccg
     2821 tcgtcgtcgg cgtcgaacac gatcgtgttc ggcaggtcgt cgatgaagcg gcagtcgttg
     2881 gccttcacct gcgcctgggg gttgatgtgc agctgggcct gcgtgcccga gccgccgcgc
     2941 ttgaacgggt cgaggccgac gcaggcgttc atcgagatct cgccccacag gcgaaagttg
     3001 cgcttgttgt cgaccgcggt cgcgcgggtc atcacggtgt cggtcgactt gaggtcgtag
     3061 ccgccatcgg tgttgccggc cgcgaaggtg tcctcgaaca cgatcgcgtg gttgtcgcgc
     3121 tcggtcgaga agccgtcggc gttccagtag ccctcgacgt cgagctggcg gttgttgagc
     3181 atcaccgcgc ggcgaaacac gacgtcgtgc gcgggcccgg cgagcgtgat ccccgtcgag
     3241 aacgactcgt attgctgggc ctccgagtcg ccgaacacgt cctcgaacag cacgtcggag
     3301 gtgtcgtatt gcaggcgcgc gaccgcgcgg gcgtagccgc ggaccgacac gtccttgagc
     3361 cgcagcccgg tgatcgccgc ctcgccctcg ccgctgacga agtcctcgag caagcgatgc
     3421 acgttgtcgg cgacgatgtt ctcgagctgc agcccggcga ccggcttggt cacgcgccag
     3481 cagccgttgc cctggttggc gaagccgagg ttgacgaagc gcaggtggtc ggcgccggcg
     3541 ccgaggcgga acacgacgcc accctcggcc ccgtcggcga cccacgggtc ggcgcgcgtg
     3601 ccgacgaggc gcgggcgacc ggcgtcgtgc cagtcggcgc cgacgccacg aatgacgacc
     3661 ggcgcgccgg cctcaccacc ggccgagatc gagatctcgc cgccgatcgc gtagtcctcg
     3721 gggccggcgt ggatccacac ctcgtccccc ggcgccgagg cgcccaccag cgcgcccagc
     3781 tggccgatcc ccgcggcgtc ggcgaggctc gcaccgctgc caccgccggc gaccgtgatg
     3841 tgccggacca tgcccgcggg cgcgtcgcgc aggccaccct cgccgaacag ctcgagcgcg
     3901 gcgagcagcg gcatcgcgct gccgggcacg cgcaccagct cgaggtccag ccactcgtcc
     3961 gccgtcaccg cgagcgtcac cgtcgcgccc gcgtcaccgg ccgtcagcgc cgagatgtcg
     4021 agggccgcga gcgcgacgac atcatcgacg cgcacgtcga cgacgcggct gccgggcccc
     4081 tgcgagggcc ccgcgaagtg caggcgcagc tgatactcgc cgggctcgac cgggatgctc
     4141 cagcgcagct gacctgtccc tccggacagc tcgtcgggct cgacgcgccc ggtcgccagc
     4201 acggccagcg gcagctccgc gggcacgctg gcgtcggggg cgacgtcctg cgtcgtgaag
     4261 atctggctgt ccgggctcgc gcggtacggc gactcggggc cgtcgtcggc gaggaagtcc
     4321 tcgaccgccg gcccgccgat gttgatgcgc accaccggtg gcccgagcgc gacgtcgccg
     4381 gtctcgctct cgccgccgct gctcgacccg tcgccgccgc ccgtcgtcgg atccggcgcg
     4441 ccgctgctgt ccatgccgcc ggtgccggtg cccgtgcccg tgctcgtgct cgtgctcggg
     4501 ccggagcccg tggtcgggtc ggggtcgtcg ccggtcgtcg gggtcgcgct ggtgccggtg
     4561 gtgcccgggc cggtgctcgc cgcgtcggtg ctgctgccgc cgtcgccctg acacgcgatc
     4621 agaaatgcca gcgcacaggg gacgaggtcg attcgggttc gcatgcgtcg cgcagcttgc
     4681 gatgcatcga ccgcgaatgt ccgcgcattc gctggttcgt cacgcggcgt cggatcacag
     4741 ggcgctttcg cgggtgtcga ccgaccccga cgacgagggc ccgacacccc cgcgattcac
     4801 cgcggctggc gacgcaggtc gtggcgatcc tgcgcgggcg gccgactgcg cgggatcttc
     4861 ggcgtgacgc ccgaccagtc tgccagcgcc gcgagggcct ggtcgcgctc cgccggcacg
     4921 aggtcgagga tgcgggcgcc gtggttctcg ccgggcgcga tgaagcgata cgtgtcctgc
     4981 gcgccttcga tctcgaaggc ggcggccgtg taggggtcgt tctcgccgta gatgaacagc
     5041 atggcgcgcc cctcggccgt cagccagtcg gcgatgtccg gcatcgccgc ggcgtcgaac
     5101 accgggacct tgcccggcag cacatagctg gcagcgacgt ccccgccggg atagaggagc
     5161 agatcggcga cgttgctctc gtcgagcgcg ggatatccga gctgcaccgc ggcctgccag
     5221 aagtagggct cgaacatctc ggtttgatag tcggaccagt aggccggcga attgtagtcc
     5281 gagatgaatg tccacagctc gtcgtcgctc gcgccggggc cgggcaccgc caggcagtcg
     5341 acctcgtcgc cgtattgcca gaacgtgaac ggcagctcga ccaccgcggt ctcgagcgcc
     5401 cggtccaggc cgagcaggtc gtaggtgtat ccatcgaccg cctgggcctg catgcgcgtg
     5461 atcatcgcgg ggcggcgcag cagcagctcg cgctggacgt cggcgagcgc ctgccggcaa
     5521 tccgcggggc cgagcccggc gacaaagtcg aggtagcgcg ggtccgagct accataactc
     5581 tgcggcgcga cgtaggcgac ggtgccgtcg acgtcctcgg gataaaaccg gcgaaaatac
     5641 acggacgtca tgccgccctt gctggccccg gtcgagatcc acttcgccgg atagaccggc
     5701 ttgaaggcgc ccgtgatcgc atgcaggtcg gcggccgcct gctcgatcgt cagggtcgcc
     5761 cagtcggccg gctcgggccg cgagggcaag aagaagcggt gctcgacgag cagctgattg
     5821 gccgccagga gcgtcgccag ctcgccgagg ccgggcttgt ccgggtcgat gaaatatccg
     5881 gcggtcgcca gcaccagcgg cgcgctgctg tcacggtgca gcagcaccag ccgctgcgcg
     5941 aacttcaggc cgtccgggtc ggcgtggtcg gccggctgca tgaaggtcat cacgaagtag
     6001 cgatatcccg cgaccggcga ctcgcgctcc tcgacctcga ggccgtcgat ctccagcaac
     6061 cgctcgagga tgtcgaccgg ctcgccggtc gtcggctcgc tcgtggtgtc cgtgctcgtc
     6121 ccgccgctcg tcggctcgct ggcggtatcg ctgccgaagg ccgtgcttgc gtcgccgctc
     6181 gtcggcaccg cgtcgtccgt ggtccccgtc gacccggtgc tcgtggcccc ggtgtcgtgc
     6241 gcgctcgtat ccgagccggt cccctggatg agattgcatg ctgtcaaaag cgcggcgcac
     6301 acgacgagcc ggagggtggc ggacattggc gcagcatagc ccggcggcgc gagcccggta
     6361 tagcccggcg gcgcgagccc ggtatagccc ggggtggcag gcaccgcgag cgcagaccac
     6421 cgcgcgctct cacgctcgct cggccgtcac gatgccccag atccagttgc gccacacgca
     6481 gtcgaggtcg acaaagccac acgcggacag catcgcgaag tgctcggcga gcggcgtgta
     6541 gtggtcgtgc gcggcggcgt ggtcgagcag gctctcaacc tcggcctcgt cgcagttgcc
     6601 gggctcgcgg cagtgctcga gccagcgccg ccagttgtgc ccgtggttgc gcgcgctgcc
     6661 gccgagctgg tcggcgaagc gcaggctgcc gccgggcgcg agcgcggcga agatgcgcgc
     6721 atacagcggg gccttgtcga ggacgtgatg caggctgatc gacgaggtca cgaggtcgaa
     6781 gccgccgggc ggcaggtcga gcgcctcgaa gcgctccgtg tggtactcgg cggcgacatc
     6841 ggggtgggcg ctcgccatgc gctcgcgcgt cgcggcgatc atctccggcg aggcgtcgac
     6901 cagcgtcagg cgagccgcgg gatagcgggc cgcgaggcgc agcgagaggt tgccggtgcc
     6961 gcagccgagc tcgaggatgc gggaggcctc gggtggcaga taatcgacga gacgcgcgct
     7021 catctcctcg taacgcggca cggcgcggcg gataaaggcg tcataggagt ccgacatggc
     7081 gccgaagtag ccggctgcgg tggtgtccct ggggtcgcgc acccgggcag catccaccct
     7141 tcgccgcggc gaggcaagcg cgcaacg
//
LOCUS       DASWNJ010000692         2677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_471774, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000692 DASWNJ010000000
VERSION     DASWNJ010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471774"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VGB85_11565"
     CDS             complement(<1..260)
                     /locus_tag="VGB85_11565"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEY0134716.1"
                     /translation="MTDTIVVYTSASDSDAAARELCERIAAEMPGAPPDALILFASPQ
                     YDQPALLRAMASRCNPKVMVGASSAGEFTSETNGTGLACALAL"
     gene            complement(376..1743)
                     /locus_tag="VGB85_11570"
     CDS             complement(376..1743)
                     /locus_tag="VGB85_11570"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY0134717.1"
                     /translation="MRWPATGIVLLTLACETAAPPQPVPAPALAPSPAPEPWSDAWLL
                     AQAPRYLDDPGARRRALERSLQNPANTYSRTRLGSYGRPDVGWDLLPEWNPRVRAVTA
                     DTAVHLTAHGEPEAADAPLWDGQRPTTMPAWVELGRRVFFGYPLRPEVFAEHALAHPE
                     VARAVGLHSAADGSVPGTVAFIDIDGRPRVGITCALCHSTVEAGAVVPGRARRDFDYG
                     AMRLAYHRDTGAPLDADMARRMTGWGPGRADITEDDDEDPVAIPDLWGLRSLGTLTQA
                     ATLRHATLGDDAPLALAIRQETQILHANRERTRPPRELAYALAMYLYALEPPPRTAVA
                     AAASERGAAIFRRDCAACHADPGGSGPPVPFADIGTDPALAGGTSRGTGLYRPAPLLR
                     VAENAPYLHHGVVPSLADLFDPARLRDDYTRGAHGPGPIAGHLPGTELAPDERDALLA
                     YLGTR"
     gene            complement(1746..2462)
                     /locus_tag="VGB85_11575"
     CDS             complement(1746..2462)
                     /locus_tag="VGB85_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134718.1"
                     /translation="MLARLTVTLLLSLAACASHEGPTPTPTPEPEPRAEVQVAIASVQ
                     LAQDCPDPASDATATPSTAGQAYAPPPPAMPADEPAAGYQSQQEPAARATAKQRMAPG
                     AAIGGTGWNPPCTQSTMQLSLANTGGRSGKLEIKAVRLLDGETKRALGPIASRKPGEW
                     NAEGTYRPWNEQIAARSTTKIAYRLAEPDWGQVQQQLGATVNLYGRPFILEVEVAVDG
                     RSQTIRSSEFMREEIHMVVT"
BASE COUNT          317 a          866 c         1137 g          357 t
ORIGIN      
        1 agcgcgaggg cgcaggccag gccggtgccg ttggtctcgg aggtgaactc gccggccgac
       61 gaggcgccga ccatcacctt cgggttgcag cggctggcca tcgcccgcag cagcgccggc
      121 tggtcgtact gcggcgaggc gaacaggatc agcgcgtcgg gcggggcccc gggcatctcc
      181 gcggcgatgc gctcgcacag ctcgcgggcg gcggcgtcgc tgtccgacgc gctggtataa
      241 acgacgatgg tgtccgtcac aggcgcctct gcgatcggtt ttactcccgt ggcgcaagaa
      301 aaggcgagcg gaacgcagtc gtttcgcttg acggagacgg attcgctgtg cgccgcggca
      361 catgcgcgcc gcgactcagc gcgttcctag ataggcgagg agagcgtcgc gctcgtcggg
      421 cgcgagctcg gtgcccggca ggtggcccgc gatcgggccg gggccgtggg cgccgcgggt
      481 gtaatcgtcg cgcaggcgcg ccgggtcgaa caggtcggcg agcgagggca cgacgccgtg
      541 gtgcaggtag ggcgcgttct cggcgacgcg caggagcggg gcggggcgat acaggccggt
      601 accgcgcgag gtgccgccgg cgagcgcggg gtcggtgccg atgtcggcga agggcacggg
      661 cggcccgctg ccgccggggt cggcgtggca ggcggcgcag tcgcggcgaa agatcgccgc
      721 gccgcgctcg ctggcggcgg cggcgacggc ggtgcgcggc ggcggctcga gcgcgtagag
      781 gtacatggcg agcgcgtacg ccagctcgcg cggcgggcgg gtgcgctcgc ggttggcgtg
      841 caggatctgg gtctcctggc ggatcgccag ggcgagcggg gcgtcgtcgc cgagcgtggc
      901 gtggcgcagc gtggcggcct gcgtcagcgt gccgagcgag cgcaggcccc agaggtcggg
      961 gatcgcgacc gggtcctcgt cgtcgtcctc ggtgatgtcg gcgcggccgg gaccccagcc
     1021 ggtcatgcgg cgcgccatgt cggcgtcgag cggggcgccg gtgtcacgat gataggcgag
     1081 gcgcatcgcc ccgtagtcga agtcgcgacg cgcgcggccc gggacgaccg cgcccgcctc
     1141 gacggtgctg tgacacagcg cgcaggtgat gccgacgcgc gggcggccgt cgatgtcgat
     1201 gaaggcgacg gtgccgggca cgctgccgtc ggcggcgctg tgcaggccga ccgcgcgggc
     1261 gacctcgggg tgggcgagcg cgtgctcggc gaagacctcg gggcgcagcg ggtaaccgaa
     1321 gaacacgcgg cgtccgagct cgacccacgc gggcatcgtc gtcggacgct ggccatccca
     1381 cagcggcgcg tcggcggcct cgggctcgcc gtgggccgtg aggtgcacgg cggtgtcggc
     1441 cgtcaccgcg cgcacgcgcg ggttccactc cggcagcagg tcccagccga cgtccgggcg
     1501 gccgtaggag ccgaggcggg tgcgggagta ggtgttggcg gggttctgca gcgagcgctc
     1561 gagggcgcgg cggcgggcgc cggggtcgtc gaggtagcgg ggcgcctgcg cgagtagcca
     1621 ggcgtcgctc cacggctcgg gtgcgggcga gggggcgagc gcgggcgcag gcacgggctg
     1681 cgggggcgcg gccgtctcgc aggcgagggt caggaggacg atgccggtcg cgggccagcg
     1741 catgctcagg tgacgaccat gtgaatctct tcgcgcatga actcggacga gcggatcgtc
     1801 tggctgcggc cgtcgacggc gacctcgacc tcgaggatga aggggcggcc gtagagattg
     1861 acggtcgcgc cgagctgctg ctggacctga ccccagtcgg gctcggcgag gcggtacgcg
     1921 atcttggtgg tcgagcgcgc ggcgatctgc tcgttccacg ggcggtacgt gccctccgcg
     1981 ttccactcgc ccggcttgcg gctggcgatc gggccgagcg cgcgtttggt ctcgccgtcg
     2041 agcaggcgga ccgccttgat ctcgagcttg ccggaacgtc cgccggtgtt ggcgagcgag
     2101 agctgcatgg tcgactgcgt gcacggcggg ttccagccgg tgccgccgat cgccgcgccg
     2161 ggggccatcc gctgcttcgc ggtggcccgc gccgcgggct cctgctggct ctggtaaccg
     2221 gcggcgggct cgtcggcggg catcgccggg ggtggcggcg cgtaggcctg gccggcggtc
     2281 gagggcgtcg cggtcgcgtc gctcgcgggg tcggggcagt cctgggcgag ctgcaccgac
     2341 gcgatcgcga cctggacctc ggcgcgtggc tcgggctcgg gggtcggggt cggcgtgggc
     2401 ccctcgtgcg aggcgcaggc ggcgagcgag agcaggagcg tgacggtcag gcgtgcgagc
     2461 atcggggacc cctcgatgcc tgcgagcatg ccaccgtgcc cgcgccggtg gaaccagcga
     2521 gatcgcgcgg gcgggtcagc gcatcgcggc gtagaacgcc agcaggccgc cctcctcgtt
     2581 gccgcacgcg ctggtcgcgg tccaggagcc gagcgcggtg ttgatgtcgt agccggcgtc
     2641 gccgtcgagc acgacggcgg agtagctgta cagatat
//
LOCUS       DASWNJ010000693         4025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_224830, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000693 DASWNJ010000000
VERSION     DASWNJ010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224830"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="VGB85_11580"
     CDS             complement(<1..885)
                     /locus_tag="VGB85_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134719.1"
                     /translation="MSATFALLLACGGGGPKSDTDTDSTTGEDTFGMGETSANPTDPT
                     SPTTPTTSASSASSATVTSASTITSASDVTTDPTITTTTTDPSDVTTDPTITISASDT
                     VTTTPPETTDPTMPVECMDPPAQEQDATCLDMSGCGCASGKCFSMPILGGWCGECLGD
                     ADCPGGGCTTPNPVASVGSVCNLGKPGDGCETDAACNDPAFAKCSPVLKVDGILTVAT
                     CGACKTNIDCAQGVKNCTPQYDLENFAGMFECVPDASVPNNEGCNLQLAGDQPIGNKA
                     CQSGFCGEANIMGLVKLGI"
     gene            complement(980..3541)
                     /locus_tag="VGB85_11585"
     CDS             complement(980..3541)
                     /locus_tag="VGB85_11585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0051731 - polynucleotide
                     5'-hydroxyl-kinase activity [Evidence IEA];
                     GO_process: GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide kinase-phosphatase"
                     /protein_id="HEY0134720.1"
                     /translation="MPVTMQLNVPDLAVVALVGATGSGKSTFARQHFKATEILNSDHC
                     RGLVSDDDNDQSATNDAFELLYGIARKRLARRLLTVIDATNVQSDSRRHILALAREYH
                     AIPVAIVLDVGAKLCLERSKTREDRKVGAHVIRQQCSLLRQSLRSLKREGFRYVYVLR
                     SPDELDSVTIERVRLWTDRRDDHGPFDIIGDIHGCFDELHELLTRLGYQIGFGDDGNY
                     YVAHPEGRRVVFLGDLVDRGPKVAEVLRLVMDMVAGGAALCVPGNHETKLLKYLRGHK
                     VKPTHGLAESIAQLEAAPPELSRRAETFIDGLVSHYMLDDGKLVVAHAGLKETLQGRG
                     SGAVREFALYGETTGETDEFGLPVRYNWAAEYRGRAVVVYGHTPVPEPEWLNRTICID
                     TGCVYGGSLTALRYPELEQVSVQAKAVYCEPSRPLRAPTERSAQQEHDDLLDLSDLLG
                     RRSIQTRLRPNLSIAAEYSSAALEVMSRFAVDPRWLIYLPPTMSPSETSERPGLLEHA
                     AEALSYFQGADIGTVVCEQKHMGSRAVIIVGQTPAALRRRFGIPTDGLGVIYTRTGRP
                     FFNDPALEQAMLARISEAATLAGLWTELATDWLCLDAELLPWSVKAQSLQRTQYATTG
                     AAGEAALAASVAVLQAATARGADVAPLLAEQQARLERVHKYRAAYRGYCWPVSSLAEH
                     RVAPFHLLASEGRAHTDQNHVWHMETLARLCAVDPGLLLATPYRVVDLEDEASRTAAI
                     HWWEELTAAGGEGMVVKPFDYIARGPKGLMQPAIKCRGREYLRIIYGPEYTAPEHLER
                     LRQRGLGGKRSLAVREFALGVEALERFVRGEPLRRVHECVFAVLALESEPVDPRL"
     gene            complement(3532..>4025)
                     /locus_tag="VGB85_11590"
     CDS             complement(3532..>4025)
                     /locus_tag="VGB85_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134721.1"
                     /translation="KHKQFTRIVGVEVAHAVLERAEQRLHLAEMPERQRARVELLHGS
                     LVYRDRRLEGFDAAVAVEVVEHIDPSRLPAFERALFASARPGLVVVTTPNAEYNVKFE
                     NLPIGAVRHHDHRFEWTRAEFAAWAEGVATRNAYTFELREIGPTDPELGAPTQMGVFR
                     RCP"
BASE COUNT          571 a         1269 c         1553 g          632 t
ORIGIN      
        1 gatgccgagc ttgacgaggc ccatgatgtt ggcctcgccg caaaagccgg actggcaggc
       61 cttgttgccg atcggctggt cgccggcgag ctggaggttg cagccctcgt tgttgggtac
      121 gctcgcgtcg ggcacacact cgaacatgcc ggcgaagttc tcgaggtcgt actgcggcgt
      181 gcagttcttg acgccctgcg cgcagtcgat gttggtcttg cacgcgccgc aggtggcgac
      241 ggtcaggatg ccgtcgacct tgagcaccgg gctgcatttt gcaaaggccg ggtcgttaca
      301 cgcggcgtcg gtctcgcagc cgtcgccagg cttgccgagg ttgcacaccg agccgacgct
      361 ggcgaccggg ttgggcgtcg tgcagccgcc gccggggcag tcggcgtcgc cgaggcactc
      421 gccacaccag ccgccgagga tcggcatcga gaagcacttg ccggaggcgc agccgcagcc
      481 gctcatgtcg aggcaggtgg cgtcctgctc ctgggcggga ggatccatac actcgaccgg
      541 catcgtcgga tcggtggtct ccgggggggt ggtcgtgacg gtgtcgctgg cgctgatcgt
      601 gatcgtcggg tcggtggtga cgtcgctcgg gtcggtggtc gtcgtcgtga tcgtcgggtc
      661 ggtggtgacg tcgctcgcgg aggtgatcgt gctcgcggag gtgaccgtcg ccgatgaagc
      721 ggaggaagcg gaggtggtcg gcgtggtcgg cgaggtcggg tcggtcgggt tcgccgaggt
      781 ctcgcccatg ccgaaggtgt cctcgcccgt ggtgctgtcg gtgtcggtgt cggacttcgg
      841 gccgccaccg ccacaggcga gcaacaatgc gaacgtcgcg ctcacgacgc ggagagaggt
      901 cggagcggtc tgcatcgcac gagagtccca cgcgcgccgc aggcccgcaa gctgcgcgcg
      961 gcgatcggtg ttttgcgtgc tacaggcgcg ggtccacggg ctcgctctcg agggcgagca
     1021 cggcgaacac gcactcgtgg acgcgacgca gtggttcgcc acggacgaag cgctcgaggg
     1081 cctcgacgcc gagggcgaac tcgcggacgg cgagcgagcg cttgccgccg agcccgcgct
     1141 ggcgcaggcg ctcgaggtgc tccggggcgg tgtattcggg gccgtagatg atgcgcagat
     1201 attcgcggcc gcggcacttg atcgccggct gcatcaggcc cttggggccg cgcgcgatgt
     1261 aatcgaaggg cttgacgacc atgccctcgc cgccggccgc ggtcagctcc tcccaccagt
     1321 ggatcgcagc ggtgcggctg gcctcgtcct cgaggtcgac gacgcggtac ggcgtggcga
     1381 gcagcaggcc ggggtcgacg gcgcacaggc gcgcgagcgt ctccatgtgc cacacgtgat
     1441 tctgatcggt gtgcgcccga ccctcgctgg cgagcaggtg aaacggcgcg acccggtgct
     1501 ccgccagcga gctgaccggc cagcaataac cgcgataggc ggcgcggtac ttgtggacgc
     1561 gctcgagccg ggcctgctgt tcggcgagca agggcgcgac gtcggccccg cgcgcggtgg
     1621 cggcctgcaa cacggcgacg gaggcggcga gggcggcctc gccggcggcg ccggtggtgg
     1681 catattgcgt gcgctgcagc gactgggcct tgaccgacca cggcagcagc tcggcgtcga
     1741 ggcacagcca gtcggtggcg agctcggtcc acagcccggc gagggtggcg gcctcgctga
     1801 tgcgcgcgag catggcctgc tcgagcgcgg ggtcattgaa gaacggccgc ccggtgcggg
     1861 tgtagatgac gccgaggcca tcggtgggga tgccgaagcg gcggcgcagc gcggcggggg
     1921 tctggccgac gatgatgacg gcgcgagatc ccatgtgctt ttgttcgcac acgacggtgc
     1981 cgatgtcggc gccctggaag taggagaggg cctcggcggc gtgctcgagc aggcccgggc
     2041 gctcgctggt ctcgctcggc gacatcgtcg gcgggaggta gatcagccag cgcgggtcga
     2101 cggcgaagcg gctcatcacc tcgagcgcgg cgctgctgta ctcggcggcg atcgagaggt
     2161 tggggcgcag gcgggtttga atgctacgcc ggccgagcag gtccgagagg tcgagaaggt
     2221 cgtcgtgctc ctgctgggcg gatctctcgg tcggggcccg cagcgggcgc gagggctcgc
     2281 aatacacggc cttcgcctgc accgatacct gctcgagctc gggatatcgc agggcggtca
     2341 gcgacccacc atacacacac ccggtgtcga tgcagatcgt gcgattgagc cactccggct
     2401 cggggaccgg ggtgtggcca taaacgacga cggcgcgccc gcggtactcg gcggcccagt
     2461 tgtagcggac cggcaggccg aactcgtcgg tctcgccggt cgtctcgccg tacagcgcga
     2521 actcccgcac cgccccggag ccgcggccct gcagcgtctc cttgaggccg gcgtgggcga
     2581 cgacgagctt gccgtcgtcg agcatgtagt ggctgacgag gccgtcgata aaggtctcgg
     2641 cgcggcggct gagctccggc ggcgcggcct cgagctgcgc gatcgactcg gcgaggccgt
     2701 gggtcggctt gaccttgtga ccgcgcaggt acttgagcag cttggtctcg tggttgccgg
     2761 gcacgcacag cgcggcgccg ccggcgacca tgtccatgac gaggcgcagg acctcggcga
     2821 ccttcgggcc gcgatcgacg aggtcgccga ggaacacgac gcggcggccc tcggggtgcg
     2881 cgacgtaata attgccgtcg tcgccgaagc cgatctggta gccgaggcgg gtcagcagct
     2941 catgcagctc gtcgaagcag ccgtggatgt cgccgatgat gtcgaacggg ccgtggtcgt
     3001 cgcggcggtc ggtccacagg cgcacgcgct cgatggtgac cgagtcgagc tcgtcgggcg
     3061 accgcaggac gtagacgtag cgaaagccct cgcgcttgag gctgcgcagc gattgacgca
     3121 ggaggctgca ttgctggcgg atgacgtgcg cgccgacctt gcggtcctcg cgggtcttgc
     3181 tgcgctcgag gcacagcttg gcgccgacat cgagcacgat cgcgaccggg atcgcgtggt
     3241 actcgcgggc gagcgcgagg atgtggcgcc ggctgtcgct ctgtacattg gtcgcgtcga
     3301 tgacggtcag caggcgccgg gcgaggcgct tgcgggcgat gccgtagagc agctcgaagg
     3361 cgtcgttggt cgcgctttga tcgttgtcgt cgtcggagac gaggccgcga caatgatcgg
     3421 agttgaggat ctccgtcgcc ttgaagtgct ggcgggcgaa ggtggacttg cccgagccgg
     3481 tcgcgccgac gagggcgacg accgcgaggt cgggcacgtt gagctgcatc gtcacgggca
     3541 tcgccgaaac actcccatct gcgtcggggc gccgagctcg gggtcggtcg ggccgatctc
     3601 ccgcagctcg aaggtataag cattgcgggt cgccacgccc tcggcccacg cggcgaactc
     3661 ggcgcgggtc cactcgaagc ggtggtcgtg gtgacgcacc gcgccgatcg gcaggttctc
     3721 gaacttgacg ttgtactcgg cgttgggggt cgtcacgacg acgaggcccg gacgcgcgct
     3781 tgcgaacagc gcccgctcga acgcaggcag ccgcgagggg tcgatgtgct cgacgacctc
     3841 gacggcgacc gcggcgtcga agccctcgag ccggcggtcg cggtagacga gcgagccgtg
     3901 cagcagctcg acgcgggccc gctggcgctc gggcatctcg gcgaggtgca ggcgctgctc
     3961 ggcgcgctcg agcacggcgt gcgcgacctc gacgccgacg atcctcgtga actgcttgtg
     4021 tttga
//
LOCUS       DASWNJ010000694         4028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_578273, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000694 DASWNJ010000000
VERSION     DASWNJ010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578273"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..171)
                     /locus_tag="VGB85_11595"
     CDS             complement(1..171)
                     /locus_tag="VGB85_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004153593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEY0134722.1"
                     /translation="MLRWALIFFIVSIVAAVFGFGGISEATADIAQILFYIFLAVFVL
                     MLVLGLVSGKRG"
     gene            complement(505..1284)
                     /locus_tag="VGB85_11600"
     CDS             complement(505..1284)
                     /locus_tag="VGB85_11600"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY0134723.1"
                     /translation="MSANNFSRDSSREGQPYITQIPARGGQSDVDQDVNYRHTRDLGQ
                     GSHVQREDPIHVDGHADASYQGYGAYATLDGSRVDESEFADPDWAGVKLEPERPVTPG
                     PGRHGGRGPRSYRRPDPRIHDQICQMLTDDSHIDASEIEVSVENGEVLLSGTVESRRL
                     KHLAEDIAYRVSGVLDVTNRLKVGALNATTRTPAPASDTQDDGPRLTNSALQARREDA
                     ELRRQGDGEDQAERARERRNLRDYNYQGTRDTSFGERLGKF"
     gene            complement(1310..1594)
                     /locus_tag="VGB85_11605"
     CDS             complement(1310..1594)
                     /locus_tag="VGB85_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134724.1"
                     /translation="MDPKKNFKPSHDVYDKDRDGPDSGVNQVNPHNTENQGGDPVKPQ
                     GGDPAEIRPQVLQVNRDAQGDEPRDIGFDGKNDAEQIKPVPGDDIDKPRP"
     gene            1924..2694
                     /locus_tag="VGB85_11610"
     CDS             1924..2694
                     /locus_tag="VGB85_11610"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEY0134725.1"
                     /translation="MPRLWVFTVLGALAIAACRDRMPASPGVVQVGATEAMVPGGLAL
                     ATARTIAVSGDAEILTAPDRFVVTVGFDLQATTLEEARDGSQKRAAALLAVTQRIPTC
                     EVQTEQLSLQPRYEGYGEPGGHRLVGYTASRSLTITLHALAEVEGLLFEMLAAGANRV
                     DRVSFQSSVLLEKRGEARLLAVEAARAKAEAMAAALGQKIGEPLKIEEAGPAPGWQTQ
                     TTNYMLDNDTTPQQSETVATGKIRVHAGVSVVFALHPG"
     gene            complement(2713..3585)
                     /locus_tag="VGB85_11615"
     CDS             complement(2713..3585)
                     /locus_tag="VGB85_11615"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0134726.1"
                     /translation="MRIHHLNCGTMCPYGGKLMGRPEPGLGPAPLACHCLLLETDRGL
                     VLVDTGLGLRDCEAPVPRLSKFFMALLRPRLDPAETAIRQIERLGFDPRDVRDIVLTH
                     LDFDHAGGLCDFPEARVHLLGAELDGARKRVSPIDRRRYRPPQWPPQANWQPYSAGGE
                     PWFGFDAVRGLAGLPPEILLVPLVGHTLGHSGIAIDTGAGWLLHAGDAYFFRDEMDPD
                     NPRCPAGLRMYQTMMQVDAKARHTNQARLRQLARAQKGQVRIHCAHDPVEFAAWSGQA
                     TPSQHSHPGESASA"
     gene            3793..>4028
                     /locus_tag="VGB85_11620"
     CDS             3793..>4028
                     /locus_tag="VGB85_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134727.1"
                     /translation="MNFLSPRIHGILDYLTAGVFALAPMFLQAEGSQVAILSCYVVAG
                     TLLVLSLLTRYPLGALRVVPFPVHGGIEVFTAAGL"
BASE COUNT          554 a         1279 c         1564 g          631 t
ORIGIN      
        1 ctagccgcgt ttgccggaca cgaggcccag caccagcatc agcacgaaga cggcgaggaa
       61 gatgtagaac aggatctgcg cgatgtcggc ggtcgcctcg gagatgcctc cgaagccgaa
      121 cacagccgcg acgatcgaga cgatgaagaa gatgagtgcc cagcgaagca tggtggtttt
      181 ctcctgactc gatctctaag ccatcttgtt cggcgcgcca gtgccaccgc gtgaaagaat
      241 gcgacgatcg ccgtcaaccg gccgccagcg gcccatggac gggcaaacga ggacagcgca
      301 gcggggccgc gggctccggg gccggggcgg ggcgcccatg acgcgaacgc gtaatgtcgg
      361 caacggcggc aatcgtatct actggcacat tcgtctccaa aagacaggca taactgcgat
      421 aagtgaccga aagaagacga ccggccgcgc ggctgcgcgg tcggtcgtcg gggaagatcg
      481 ccagagtggt tcgccgggcg atcgtcagaa cttgccgagg cgctcgccga acgaggtgtc
      541 gcgggtgccc tggtagttgt agtcgcgcag gttcctgcgc tcgcgggccc gctcggcctg
      601 gtcctcgccg tcgccctggc ggcgcagctc ggcgtcctcg cggcgggcct gcagcgcgct
      661 gttggtcagg cgggggccgt cgtcctgcgt gtcgctggcg ggggccggcg tacgcgtggt
      721 ggcattgagc gcgccgacct tgaggcggtt ggtcacgtcg agcacgccgg acacgcggta
      781 ggcgatgtcc tcggcgaggt gcttgaggcg gcggctctcg acggtgcccg agagcaggac
      841 ctcgccgttc tcgacggaga cctcgatctc gctggcgtcg atgtgcgagt cgtcggtcag
      901 catctggcag atctggtcgt ggatccgcgg gtccgggcgg cggtagctcc ggggcccacg
      961 accgccgtga cggccggggc ccggggtgac cgggcgctcg ggctcgagct tgacgccggc
     1021 ccagtcgggg tcggcaaact cgctctcgtc gacgcggctg ccgtcgagcg tcgcgtaggc
     1081 gccgtagccc tgataggagg cgtccgcgtg gccatcgacg tggatcgggt cctcgcgctg
     1141 gacgtgcgag ccctggccga ggtcgcgggt gtggcggtag ttgacgtcct gatcgacgtc
     1201 gctctgcccg ccgcgcgcgg ggatctgtgt gatgtacggc tgaccctcgc gggagctgtc
     1261 cctggagaag ttgttggctg acatggagac ctcgggggtg aggggagcgc tacggacggg
     1321 gcttgtcgat gtcgtcgccg gggacgggct tgatctgctc ggcgtcgttc ttgccgtcga
     1381 agccgatgtc ccggggctcg tctccctgcg cgtcacggtt gacctgcagc acctgcggcc
     1441 ggatctcggc cgggtcgccg ccctgcggct tcacggggtc gccaccctgg ttctccgtgt
     1501 tgtgcgggtt gacctgattg accccgctgt ccggcccgtc gcggtccttg tcgtaaacgt
     1561 cgtgcgaggg cttgaagttc ttcttcgggt ccatggctcg tggtcctttg cagtgcgcga
     1621 tcggcccagc acgtgctgca atgcggaccg acaggggccc tcgcagctgc acgcgtgtgc
     1681 tcgatggtgt gtgttgacct tagaaaaggt cctggtgttg tcaaggcgag gacgcgcgac
     1741 cgtgtgccgc ggcgccggag ccgtccacga ggcccctgta ccggaggtta tgcggccggc
     1801 accgatcgag gcgcgcaggc tgcgaggtcg cgtcgaggac cggagcgcag gcggcgcccg
     1861 gtcctgcgat cattttacgt ggaccttacc gccgaccccg gggcgccgcg gtagcttgct
     1921 cgtatgccga ggttatgggt gttcacggtt ttgggggccc tggcgatcgc tgcgtgtcgc
     1981 gaccgcatgc cggcgtcgcc gggggtggtg caggtcggcg cgacggaggc gatggtcccc
     2041 ggtgggctcg cgctggcgac ggctcgcacg atcgccgtca gcggcgacgc ggagatcctg
     2101 acggcgccgg accgcttcgt cgtgacggtg ggcttcgacc tgcaggcgac cacgctcgag
     2161 gaggcgcgcg acggcagcca gaagcgggcc gcggcgctgc tggcagtgac gcagcggatc
     2221 cccacctgcg aggtgcagac cgagcagctg tcgttgcagc cgcgctacga ggggtacggt
     2281 gagccgggcg ggcatcgcct cgtcggctac acggcgtcgc gcagcctgac gatcacgttg
     2341 cacgcgctcg cggaggtcga ggggctgctg ttcgagatgc tggcggcggg ggccaaccgg
     2401 gtcgaccggg tcagctttca gagcagcgta ttgctcgaga agcgcggcga ggcgcggctg
     2461 ctcgcggtcg aggcggcgcg cgccaaggcc gaggcgatgg cggcggcgct cggacagaag
     2521 atcggcgagc cgctcaagat cgaggaggcc gggccggcgc caggctggca gacgcagacg
     2581 acgaattaca tgctcgacaa cgacacgacg ccgcagcaaa gcgagacggt cgcgaccggc
     2641 aagatccgcg tgcacgcggg cgtgtcggtg gtgttcgccc tccaccccgg gtgagtcgcc
     2701 ggggtgaggc ggtcaggcgc tggcggactc gccggggtgg ctgtgctgcg agggggtggc
     2761 ctggcccgac caggccgcga actcgaccgg gtcgtgggcg cagtggatcc ggacctggcc
     2821 cttctgggcg cgggcgagct ggcgcaggcg ggcctggttg gtgtgccggg ccttcgcgtc
     2881 gacctgcatc atcgtctggt acatgcgcag accggcgggg cagcgcgggt tgtcggggtc
     2941 catctcgtcg cggaagaagt aggcgtcgcc ggcgtgcagc aaccagccgg cgccggtatc
     3001 gatggcgatg ccgctgtgcc cgagcgtgtg accgacgagc ggcaccagca ggatctcggg
     3061 cgggaggccg gcgaggccgc gcacggcgtc gaagccgaac cacggctcgc cgccggcgct
     3121 gtagggctgc cagttggcct gcgggggcca ttgcgggggc cggtagcggc ggcggtcgat
     3181 cggggagacg cgcttgcggg cgccgtcgag ctcggcgccg agcaggtgca cgcgggcctc
     3241 ggggaagtcg cacaggccgc cggcgtggtc gaagtcgagg tgggtgagga cgatgtcccg
     3301 gacgtcgcgc gggtcgaagc cgaggcgctc gatctggcgg atcgcggtct cggcggggtc
     3361 gagccggggg cgcagcaggg ccatgaagaa cttgctcagg cggggcaccg gcgcctcgca
     3421 gtcgcgcagg ccgagaccag tgtcgacgag cacgaggccg cggtcggtct ccagcaggag
     3481 acagtgacag gcgagcgggg ccgggccgag gccgggctcg gggcgaccca tgagcttgcc
     3541 gccgtagggg cacatggtgc cgcaattgag gtgatggatc cgcatgcgtt cctcccccgg
     3601 gtctgtggtc gatcgccgcg agcttgcaag cgcatcgggc ggagacgacc agcggaggcc
     3661 gcgcaggacc gccaggatcc gccggacagc acgggttcgg ccgccggggc gcccgtgtcg
     3721 gtgcaggtcg cgttgccggg cgcgatgctg cccccataag acctgcaccc cgccacccaa
     3781 ggacgctccg ccatgaattt cctgtcccca cgcatccacg gaatcctcga ctacctcacc
     3841 gccggcgtgt tcgcgctggc cccgatgttc ctgcaggccg agggctcgca ggtcgcgatc
     3901 ctcagctgtt acgtggtcgc cgggacgctg ctcgtgctct cgctgctgac gcgctatccg
     3961 ctgggcgcgc tgcgggtcgt cccgttcccg gtgcacggcg gcatcgaggt cttcacggcc
     4021 gccggcct
//
LOCUS       DASWNJ010000695         4689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_578288, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000695 DASWNJ010000000
VERSION     DASWNJ010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578288"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VGB85_11625"
     CDS             <1..195
                     /locus_tag="VGB85_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134728.1"
                     /translation="LPSVDEAVRQAEAALAAGALDPVTAREVAARRVAARRLHLAAMY
                     ERREAIIALEAAVGGPLARP"
     gene            216..1535
                     /locus_tag="VGB85_11630"
     CDS             216..1535
                     /locus_tag="VGB85_11630"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEY0134729.1"
                     /translation="MSLRFAPLSLSLLLLTIACGDAGSQTTADTPATGSDSSGDTPTT
                     DATTPTTGSSGEQGECGDGKLDAGEVCDGADLGGKQCADLDFLAGTLACAGDCGSFDV
                     SGCEQDPSAALVVLNEVSAKGASEGPYADKGDAIELYNAGGATAELAGWKLSDDPTFP
                     IEKTYVFPPGSSLAPEAYLVLVVYDEVTMTGDLPFGISTTKEETITLTNAADEMADQL
                     IVQGADATVSYCRVPDGTGAWQGCDLTLGGPNLSASQTCGDGKIDGTEVCDGAALDGA
                     TCQSLGFTGGTLACAGTCTLDASMCDSGSEVAINELEATDDQIEIHNAGGAAVDISGW
                     ILTDDEVDANYDPAADPERLVFPDASSLAAKEFLVVAKGMLAGQHPFGLGATGDTVTL
                     LDADLKVISQVTYADGQAATSFCRTPDGPTGMWAADCVPTLGSANMK"
     gene            1618..3138
                     /locus_tag="VGB85_11635"
     CDS             1618..3138
                     /locus_tag="VGB85_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134730.1"
                     /translation="MSRSSRRFITLPLVSWVVSCGPIDPEDAVAIRFDPETYALKVGE
                     TANAKILAVDDGRGSLDVTAEASLEVVSGALSIDATGVITATAPSFGTVRATWMDFEA
                     TAPVRIGGGWMPAEFPDIELPSTSTDALVSVAPNDHVAILLNDQQIPRITLLDPATGF
                     TTPEPVGDATGFGEGVALRSDGGVLVVVREGGDAPTLGSLERAPDGTWTRLGRIEPGT
                     SHPWRVTGFGANDSGEAAVVLEGYNAPGLGFARRAAGASEWEVVDVGVEDYAWTSSVV
                     VNGEGVVRFAHAPYNLEPPVLNLYRAARGEALQVALTITSDLSIDCPLLAVDAEGDGW
                     LAWFDSTPESTTRMRVAELRGDAVVGGPYDLPGDFYIAGSCEPQLVLDAEGRGLLLAV
                     PRVGDPAAAAPFRVLEPGKPLPAGLAVSDDAGHLQSSHMAVTGAGDVWFEWYTDAGRR
                     VRRLRRDGTFEPSSVWPVPVYVTLTQVFAGLRTGAIGVATTYENDSHGALVMTWLE"
     gene            complement(3140..3790)
                     /locus_tag="VGB85_11640"
     CDS             complement(3140..3790)
                     /locus_tag="VGB85_11640"
                     /inference="COORDINATES: protein motif:HMM:NF012409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004568 - chitinase activity [Evidence
                     IEA];
                     GO_process: GO:0006032 - chitin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HEY0134731.1"
                     /translation="MRRLGPASVMCLSLACSDPGGGETGGGATGSGETGGGAASTSDA
                     GATTGTNATSPPGTATDPGDTSADPSDPNNAQYPCAPGKTYHGRGPIQLSWNYNYGPA
                     GEAIGEPLLAQPELVTSDGAIAFRTGLWFWMTAQAPKPSAHDVMVGAWQPSAEDQAKG
                     RTPGFGMTINIINGGLECGQPTSAKVEDRVGFYDRFTGMLAVDMGPALYCDTMQPY"
     gene            3886..>4689
                     /locus_tag="VGB85_11645"
     CDS             3886..>4689
                     /locus_tag="VGB85_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134732.1"
                     /translation="MSKPDHARLWQRVREGQPTALIGRLPDSPQDLHLLRVSCEVAPA
                     ALRPLLEARRLTEAVLGDVRRPEKAKSVRRRTLFGDTPERATEAVVVELWNQLAKQKG
                     VRWVVVFDAVEQADEATLAALAQVVRRPGWMLLPLVLVFHGEPTGPAAELLAAVRTRG
                     EDAVLSGEAPPRDAGATIEWRALPPEVLRVLRAGALIGPGFETRIVADQLAVEPLVVL
                     EQLQQASDLGVPLEDRGEGRFSLPTSALTALTASLMPSMAQAWHRKLGRL"
BASE COUNT          624 a         1728 c         1687 g          650 t
ORIGIN      
        1 ctgccgagcg tcgacgaggc ggtgcgtcag gccgaggcgg cgctggccgc cggcgcgctc
       61 gaccccgtga ccgccagaga ggtcgcggcg cggcgcgtag cggcgcgccg gctgcacctc
      121 gcggcgatgt acgagcgccg cgaggcgatc atcgcgctcg aggcggcggt cgggggcccg
      181 ctcgcccggc cgtgacctta tactccggcc cgcttatgtc gcttcgcttc gcccctctgt
      241 ccctctcgct cctcctcctc accatcgctt gcggcgacgc cggctcgcag accaccgcgg
      301 acacgccggc gaccggcagt gacagcagcg gcgacacgcc gaccacggac gcgaccacgc
      361 cgacgaccgg atcgagcggc gagcagggcg agtgcgggga cggcaagctc gacgccggcg
      421 aggtgtgcga cggggccgac ctcggcggca agcagtgcgc cgacctcgac ttcctcgccg
      481 gcacgctcgc gtgcgcgggc gactgcggct cgttcgacgt ctccggctgc gagcaggacc
      541 cgtcggccgc gctggtggtg ctcaacgagg tcagcgcgaa gggggcgagc gagggcccct
      601 acgccgacaa gggcgacgcg atcgagctgt acaacgccgg cggcgcgacg gccgagctcg
      661 cgggctggaa gctgtccgac gacccgacgt tcccgatcga gaagacctac gtgttcccgc
      721 cgggcagctc gctcgcgccc gaggcctacc tcgtgctcgt cgtgtacgac gaggtcacga
      781 tgaccggcga tctgccgttc ggcatcagca cgaccaagga ggagacgatc accctgacga
      841 acgcggccga cgagatggcc gaccagctga tcgtccaggg cgccgacgcg acggtgtcgt
      901 actgccgcgt gcccgacggc accggcgcgt ggcagggctg cgacctgacg ctcggcgggc
      961 ccaacctctc ggccagccag acctgcggcg acggcaagat cgacggcacc gaggtctgcg
     1021 acggcgcggc cctcgacggc gcgacctgcc aatctctcgg cttcaccggc ggcaccctgg
     1081 cctgtgcggg cacctgcacg ctcgacgcgt cgatgtgcga ctcgggcagc gaggtcgcga
     1141 tcaacgagct cgaggcgacc gacgaccaga tcgagatcca caacgccggt ggcgccgcgg
     1201 tcgacatctc gggctggatc ctgaccgacg acgaggtcga cgccaactac gaccccgccg
     1261 ccgaccccga gcggctggtg ttcccggacg cgagcagcct cgccgccaag gagttcctgg
     1321 tcgtcgccaa gggcatgctc gccggccagc accccttcgg cctcggggcg accggcgaca
     1381 cggtcacgct gctcgacgcc gacctcaagg tcatcagcca ggtcacctac gccgacgggc
     1441 aggcggcgac gtcgttctgc cgcacgcccg acggcccgac cggcatgtgg gcggcggact
     1501 gcgtcccgac gctcggcagc gcgaacatga agtgatgcct gcgagtgcag gcatgctcgc
     1561 gagtgcgcac atacgcctcg cggcgcgtga ctcgccggcg ccgtgttagc ctgcgggatg
     1621 tccaggtcct cgcgccggtt catcaccctg ccgctcgtct cgtgggtcgt gtcatgcggc
     1681 ccgatcgacc ccgaggacgc ggtcgcgatc cgcttcgatc ccgagaccta cgccttgaag
     1741 gtcggcgaga ccgcgaacgc gaagatcctc gcggtcgacg acggccgcgg cagcctcgac
     1801 gtcaccgccg aggcgagcct cgaggtcgtc agcggcgcgc tgtcgatcga cgcgaccggc
     1861 gtcatcacgg cgacggcgcc gagcttcggg accgtgcgcg cgacgtggat ggacttcgag
     1921 gcgaccgccc cggtgcggat cggcggcggc tggatgccgg ccgagttccc cgacatcgag
     1981 ctgccgagca cctcgaccga cgcgctggtc tcggtggcgc cgaatgacca cgtggcgatc
     2041 ctcctcaacg atcaacagat cccgcggatc accctgctcg acccggcgac cggcttcacg
     2101 acgccggagc ccgtcggcga cgcgaccggc ttcggcgagg gcgtcgcgct gcgcagcgac
     2161 ggcggcgtgc tggtggtcgt ccgcgagggc ggtgacgcac cgaccctcgg ctcgctcgag
     2221 cgcgcaccgg acggcacctg gacccgcctc gggcgcatcg agcccggcac ctcgcatccg
     2281 tggcgcgtca ccgggttcgg cgcgaacgac tccggcgagg ccgcggtcgt gctcgagggc
     2341 tacaacgcgc cgggcctcgg cttcgcccgg cgcgcggccg gggcgagcga gtgggaggtc
     2401 gtcgacgtcg gcgtcgagga ctatgcatgg acatcgtcgg tcgtggtcaa cggcgagggc
     2461 gtggtccgct tcgcccatgc gccatacaac ctcgagcctc cggtgctcaa cctctaccgc
     2521 gcggcgcgtg gcgaggccct gcaggtcgcg ctgacgatca cgtccgacct ctcgatcgac
     2581 tgcccgctgc tggcggtcga cgcggagggg gacggctggc tcgcgtggtt cgattcgacg
     2641 ccggagtcca cgacgaggat gcgggtcgcc gagctgcgcg gcgacgcggt cgtcggcggg
     2701 ccgtacgacc tgcccggcga cttttacatc gccggctcgt gcgagcccca gctcgtgctc
     2761 gacgccgagg gccgtggtct cttgctcgcg gtcccgcggg tcggagatcc cgccgcggcc
     2821 gccccgttcc gcgtgctcga gcccggcaag ccgctgcccg ccgggctcgc cgtcagcgac
     2881 gacgccgggc acctgcagtc gtcgcacatg gcggtgaccg gcgcgggcga cgtgtggttc
     2941 gagtggtaca ccgatgccgg acggcgcgtg cgtcggctcc ggcgcgacgg caccttcgag
     3001 ccgtcgagcg tgtggccggt gcccgtgtac gtcacgctga cgcaggtgtt cgccggcctg
     3061 cgcacgggcg cgatcggtgt cgccaccacc tacgagaacg actcccacgg cgcgctggtg
     3121 atgacctggc tcgagtgact cagtacggct gcatcgtgtc gcagtacagg gccgggccca
     3181 tgtcgaccgc gagcatcccg gtgaagcgat cgtaaaaacc gacgcggtcc tcgaccttgg
     3241 cgctggtcgg ctgcccgcac tcgagcccgc cgttgatgat gttgatcgtc atgccgaagc
     3301 ccggcgtgcg acccttggcc tggtcctccg cgctcggctg ccacgcgccg accatgacgt
     3361 cgtgcgcgga gggcttgggc gcctgcgcgg tcatccagaa ccatagcccg gtgcgaaacg
     3421 cgatcgcgcc gtcgctggtg accagctccg gctgcgcgag caagggctcg ccgatcgcct
     3481 cgccggccgg cccataattg taattccagc tgagctggat cggcccgcga ccgtgatagg
     3541 tcttgcccgg cgcgcacgga tactgcgcgt tgttcgggtc gctcgggtcc gcgctcgtat
     3601 cgcccgggtc ggtcgcggtc cccggagggc tggtcgcgtt cgtgcccgtg gtggcgcccg
     3661 cgtcgctggt gctcgccgct ccaccgcccg tctcaccact gcccgtcgca ccgccacccg
     3721 tctcaccgcc acccggatcg ctgcacgcca ggctcaggca catcacgctc gcgggtccca
     3781 gtcgtcgcac gtgaggacgg tacccgctct cgccgagatc gtcgaccgcg ggtgggtgcc
     3841 ggaagccacg cgacgcgccg cggcggcttc gttaccatcg ctgccatgtc caagcccgac
     3901 catgcccgtc tgtggcagcg cgtgcgcgag ggccagccga ccgcgctgat cggccggctg
     3961 cccgactcgc cccaggacct gcacctgctg cgcgtcagct gcgaggtcgc acccgcggcc
     4021 ctgcggccgc tgctcgaggc ccgacgcctg accgaggccg tgctcggcga cgtgcgaagg
     4081 cccgagaagg ccaagtcggt gcgccgccgg accctgttcg gcgacacccc cgagcgcgcg
     4141 accgaggccg tcgtcgtcga gctgtggaac cagctcgcca aacaaaaggg cgtgcgctgg
     4201 gtggtcgtgt tcgacgcggt cgagcaggcc gacgaggcga cgctcgccgc gctggcgcag
     4261 gtcgtacgcc ggccggggtg gatgctgttg ccgctggtgc tggtgttcca cggcgagccg
     4321 accggcccgg cggcggagct gctggcggcc gtgcgcacgc gcggcgagga cgcggtgctg
     4381 tccggcgagg cgcccccgcg cgacgccggc gcgacgatcg agtggcgcgc gctgccgccg
     4441 gaggtgctgc gggtgctgcg agccggcgcc ctgatcggcc cgggcttcga gacgcggatc
     4501 gtcgccgacc agctggcggt cgagccgctg gtcgtgctcg agcagctgca acaggccagc
     4561 gacctcggcg tcccgctcga ggaccgcggc gagggccgct tctcgctccc gacctccgcg
     4621 ctgacggccc tgaccgcgtc gctgatgccc tcgatggccc aggcctggca ccgcaagctc
     4681 ggccgcctg
//
LOCUS       DASWNJ010000696         2896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_260858, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000696 DASWNJ010000000
VERSION     DASWNJ010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260858"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..1268
                     /locus_tag="VGB85_11650"
     CDS             261..1268
                     /locus_tag="VGB85_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0134733.1"
                     /translation="MQTLIIGDIHGCHAELLDLLAAAGIGDEDLVVSVGDLVDRGPDP
                     GAVLDFFRARNNSVVLCGNHERKHIRGVLSYSQQVTKAQLGDRYEDHVRWMAGLPYHW
                     EREDVRVVHWGLYPGVPLDEVPEDVRAGTTSGDTKLREKFGDRPWYDFYTDAKPVVFG
                     HAVVGAQPLVIDDRVFGLDTGACHGMRLTGLLLPALRLVSVPARADHWETVRKQWQAP
                     VLRTLPWTGMTFEQIDKKLRSLRDPELGDEVLDAIAAWATTLRAAIPGLRDRLDAEVA
                     RLAAESEDFGRAASQLPAGSWLIRRRTGKLSPEHLGCAGPQQLITLAAALGVELPAAP
                     L"
     gene            1406..2122
                     /locus_tag="VGB85_11655"
     CDS             1406..2122
                     /locus_tag="VGB85_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134734.1"
                     /translation="MCSKEERLWRLLTTLFHDDQQLRRFIALGQDSREITTLLPGAGA
                     SLTDLSLKVVELLSARGTVPAMLEHLATWHLDCTHGEAIAELQRSWNGDPRAEGAMQH
                     EKRHTRLRLPAGADLDVLPYIKGWSQLRRPHTWLTLRLTGSTLEIDAFPYLLRRGAVV
                     HGPVSYAVGERASDDTSDPPAPDPCEQRRRSTYSYYPLGRGHGPAFDLTLGDQVLLLD
                     SPGTGETDASVQTLVIEYDL"
     gene            2234..>2896
                     /locus_tag="VGB85_11660"
     CDS             2234..>2896
                     /locus_tag="VGB85_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134735.1"
                     /translation="MQPQKLFINREWYLEVTSEGYVHGPCFEGAFGCVIPIHAESGSG
                     DGPDRLALKIPRLMADTVRENEFIRHIVETEAEFVRKANREMNYNTGLVPIERLQFGL
                     LRGVRELRGNADDPRTRQDGNIILISFPKDRPPRIVSCRIDAATGELHVFPGGARSDL
                     SFVKRELWERMRDPNPRAGVAQNAYGTDFGEPFFVEVTGQSHTSERTTQGPLRTTLIG
                     EAE"
BASE COUNT          445 a         1100 c          929 g          422 t
ORIGIN      
        1 cccgcaggcg ccgcagcgcg cgggtgatgc tctcgacgtc ctcgcgccac ccgtgggtcg
       61 ccgtcaatcg ctcgctgacg gcgacgagcg agccgtgctc ggcgatcagg ccgtcgaggt
      121 acacccccca ggtcggctgt cctgcatttt gtccgggccc cggcatcgcc aggtcggtat
      181 atcaaccctg cgtggcgacg gggccgggcg tggcgacgat cgccacctcc gggcctggat
      241 ccgcgcatgg aggcactgtc atgcaaactc taattattgg cgacatccac ggctgtcacg
      301 ccgagctcct cgacctgctc gccgcggcgg ggatcggcga cgaggacctc gtcgtcagcg
      361 tcggcgacct cgtcgaccgc ggccccgacc ccggggccgt gctcgacttc ttccgcgcgc
      421 ggaacaacag cgtcgtcctg tgcggcaacc acgagcgcaa gcacatccgc ggcgtgctct
      481 cgtactcgca gcaggtcacc aaggcccagc tcggcgatcg ctacgaggac cacgtgcgct
      541 ggatggccgg actgccgtac cactgggagc gtgaggacgt gcgcgtggtc cactggggcc
      601 tgtaccccgg cgtgccgctc gacgaggtcc ccgaagacgt gcgcgccggc acgacctccg
      661 gcgacaccaa gctgcgcgag aagttcggtg accgcccctg gtacgacttc tacaccgacg
      721 ccaagccggt cgtgttcggt cacgccgtcg tcggcgcgca gcccctcgtg atcgacgacc
      781 gcgtgttcgg cctcgacacc ggggcctgcc acggcatgcg cctgaccggc ctgctgttgc
      841 ccgccctgcg cctcgtctcg gtgccggccc gcgccgacca ctgggagacg gtgcgcaagc
      901 aatggcaggc gccggtgctg cgcacgcttc cgtggaccgg catgaccttc gagcagatcg
      961 acaagaagct gcggtcgcta cgcgacccgg agctcggcga cgaggtgctc gatgcgatcg
     1021 ccgcgtgggc gacgaccctg cgcgctgcga tccccgggtt gcgcgaccgc ctcgacgccg
     1081 aggtcgcgcg cctcgccgcc gagtccgagg acttcggccg cgccgccagc cagctgcccg
     1141 cgggctcgtg gctgatccgc cggcgcaccg gcaagctctc gccggagcac ctcggctgcg
     1201 ccggcccgca gcagctgatc accctcgccg ccgccctcgg cgtcgagctc ccggccgcgc
     1261 cactgtgaag atttgatcac accccggcgg acggggcgcg gccaggccgc gccccgtccg
     1321 cttatattta tattgagaat tgtgctgaca ttgtgatcga agcaagcacc ctggaacact
     1381 cttatgcaaa gcccgggagg ggtccatgtg ctcaaaagaa gagaggctgt ggcggctgct
     1441 gaccacgctg tttcatgacg accagcagct gcgtcgtttc atcgcgctcg gccaggacag
     1501 ccgggagatc acgacgctgc tccccggcgc cggcgcctcc ctgaccgacc tcagcctcaa
     1561 ggtcgtcgag ctgctctcgg cgcgaggcac cgtgcccgcg atgctcgagc acctggccac
     1621 ctggcacctg gattgcacac acggcgaggc gatcgccgag ctccagcgat cctggaacgg
     1681 cgacccacgc gccgaagggg ccatgcaaca cgagaagcgc catacccgtc tgcgcctgcc
     1741 ggccggggcc gacctcgacg tcttgccata tatcaagggc tggtcgcagc tgcgccggcc
     1801 gcatacctgg ctgaccctgc gcttgaccgg gtccaccctc gagatcgacg cgttcccgta
     1861 cctgcttcgc aggggcgcgg tcgttcatgg cccggtctcg tacgccgtcg gtgagcgcgc
     1921 gtcggacgac acgagcgacc cgcccgcgcc cgacccatgc gaacagcggc gccgctccac
     1981 ctactcctat taccccctcg gccgcgggca cgggccggcc ttcgatctca cgctcggcga
     2041 tcaggtgttg ctgctcgaca gccccggcac cggcgagacc gacgcgtccg tccagaccct
     2101 cgtgatcgag tacgacctct gaccggcaag gagacccatg acgatcccac cgcctccccg
     2161 ctccgtccgc ccgcccccga ccccgactcc ggaccctccg accgccccgc ggctacggtc
     2221 ctccgcggtc cgcatgcagc cccagaagct gttcatcaac cgcgagtggt acctcgaggt
     2281 gaccagcgag ggttacgtgc acggcccgtg cttcgagggc gcgttcgggt gcgtcatccc
     2341 gatccacgcc gagtccggca gcggcgacgg ccccgaccgc ctcgcgctga agatcccgcg
     2401 cctgatggcg gatacggtcc gcgagaacga gttcatccgc cacatcgtcg agaccgaggc
     2461 ggagttcgtg cgcaaggcca accgggagat gaactacaac accggcctgg tcccgatcga
     2521 gcgcctgcaa ttcggcctgc tccgcggcgt ccgcgagctg cgaggcaacg ccgacgaccc
     2581 gcgcacgagg caggacggca acatcatcct gatctccttc cccaaggacc gcccgccgcg
     2641 gatcgtcagc tgccggatcg acgcggccac cggcgagctg cacgtcttcc ccggcggggc
     2701 ccgcagcgac ctctccttcg tcaaacgcga gctctgggag cgcatgcgcg accccaaccc
     2761 gcgcgccggt gtcgcccaga acgcctacgg cacggacttc ggcgagccct tcttcgtcga
     2821 ggtcaccggg cagagtcata cgtccgagcg gacgacgcag ggccccctgc gaaccaccct
     2881 catcggcgag gccgag
//
LOCUS       DASWNJ010000697         1873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_82456, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000697 DASWNJ010000000
VERSION     DASWNJ010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82456"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VGB85_11665"
     CDS             complement(<1..236)
                     /locus_tag="VGB85_11665"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134736.1"
                     /translation="MLGARETSVERWADLGVLRCVPATGERRRFARDEVERFAEAVRA
                     GHPDMTLLDPPAPLEASLPLEPAVERWTDRLLTL"
     gene            complement(546..>1873)
                     /locus_tag="VGB85_11670"
     CDS             complement(546..>1873)
                     /locus_tag="VGB85_11670"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEY0134737.1"
                     /translation="AGPFNPITDNADPLYYTGIMQREGKNGDLYVQTFGWPVHARRWL
                     PSHDHPRDIATFSADVGIDNDYMVLANGVPVRSGEADGLQRTAFVLQQPVPTYAMHVI
                     ASDFEVVRLGVVDGVAIDAVVHAAEKPLVHELWSATVTALGYYNEVLGAFPFSRYAMI
                     SVPGAFGGMEHATIVSLADTGVVANGNSTRRAAIHELSHHWFGDSAHQGEWEAFWLNE
                     SLAAFMELEGIRILGGGAAYRMRLDALRTALFGNADTYNDDALHYKVPQEFPGQVTAA
                     SFNAPYVKGPWLFHMLRVQLGDQKMADFLRAVYEQHRFAPYTTESVLAVLNATSGEDY
                     SQFFDEWVYMAGWPQVQVDWVYNDVSKQVEISVEQVQDIIKFGTYTLEGGLDLDLVFD
                     DGDAMTPPCEVSVAFATDETLVESTLDCAQAPTGFTLPPQSNVLVELVP"
BASE COUNT          302 a          571 c          696 g          304 t
ORIGIN      
        1 tcgagcgtca gcagccgatc ggtccagcgc tcgacggcgg gctcgagggg cagtgacgct
       61 tcgagcgggg ccggcgggtc gagcagggtc atgtccggat gccccgcgcg cacagcctcg
      121 gcgaagcgct cgacctcgtc acgcgcgaag cggcggcgct cgccggtcgc aggtacgcag
      181 cgcaggaccc cgaggtcggc ccagcgctcg accgaggtct cgcgcgcgcc cagcatgcgc
      241 gccacctcgc ccgccgacag cagccgctcg cggcgagagc gacgccgaag ttgaaccagg
      301 gggcgggcca gatggcgacg cagtggggaa tgcgtacgca tccgtcgaca gccacaatag
      361 tcgatcgggg gaattactag tgtgatacgg agcaagatta cacgattatt ttttcatagg
      421 ttatcgctgg tcggttgatg agacttatcg ctgatccgcc gttcaacgta cgaaaggatg
      481 atcgccgcgc cggccagcgc gaatttgttg gtcgcggtgg ccggcgcgaa agcgcgaggg
      541 ccgggtcagg gcaccagctc gacgagcacg ttgctctggg ggggcagcgt gaagccggtc
      601 ggggcctggg cgcaatcgag ggtgctctcg acgagcgtct cgtcggtggc gaaggcgacc
      661 gagacctcgc acggcggggt catcgcgtcg ccgtcgtcga acacgaggtc gaggtcgagg
      721 ccgccctcga gggtataggt gccgaacttg atgatgtcct ggacctgctc gacgctgatc
      781 tcgacctgct tgctgacgtc gttgtagacc cagtcgacct ggacctgcgg ccagccggcc
      841 atgtagaccc actcgtcgaa gaactggctg taatcctcgc cgctggtcgc gttgaggacc
      901 gcgagcacgg actcggtggt gtacggggcg aagcggtgct gctcgtagac ggcgcgcagg
      961 aagtcggcca ttttttgatc gccgagctgt acgcgcaaca tgtggaacag ccacgggccc
     1021 ttgacgtagg gcgcattgaa ggacgccgcg gtgacctggc cggggaactc ctgcgggacc
     1081 ttgtagtgca gcgcgtcgtc gttgtaggtg tcggcgttgc cgaacagggc ggtgcgcagg
     1141 gcgtcgaggc gcatgcggta ggccgccccg ccgccgagga tccggatgcc ctcgagctcc
     1201 atgaaggcgg ccaggctctc gttgagccag aacgcctccc attcgccctg atgcgcgctg
     1261 tcaccgaacc agtggtggct cagctcgtgg atcgcggcgc ggcgggtgct gttgccgttg
     1321 gcgaccacgc cggtgtcggc gagcgagacg atcgtcgcgt gctccatccc gccgaaggcg
     1381 ccgggcacgg agatcatcgc gtaacgcgag aaggggaacg cgccgaggac ctcgttgtaa
     1441 tagccgagcg cggtgacggt ggcgctccac agctcgtgga cgagcggctt ctcggcggcg
     1501 tgcacgacgg cgtcgatagc gacgccgtcg acgacgccga ggcgcacgac ctcgaagtcg
     1561 gaggcgatca cgtgcatcgc gtaggtcggg accggctgct gcaggacgaa ggcggtgcgc
     1621 tgcaggccgt cggcctcgcc gctgcgcacc ggcacgccgt tggcgaggac catgtagtcg
     1681 ttgtcgatcc cgacgtcggc gctaaacgtg gcgatgtcgc gggggtgatc gtggctcggc
     1741 aaccaccggc gggcgtgcac cggccagccg aaggtctgga cgtagaggtc gccgtttttg
     1801 ccctcgcgct gcatgatgcc ggtgtaatag agcgggtcgg cgttgtcggt gatcgggttg
     1861 aaggggcccg cgt
//
LOCUS       DASWNJ010000698         2869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_578302, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000698 DASWNJ010000000
VERSION     DASWNJ010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578302"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1542
                     /locus_tag="VGB85_11675"
     CDS             <1..1542
                     /locus_tag="VGB85_11675"
                     /inference="COORDINATES: protein motif:HMM:NF016190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEY0134738.1"
                     /translation="ARPLAILAAISIVALVALDAAARPGGGHGYSGGGGGGHSGGGGY
                     SGGGGYSGGSHSSSGGGGDAGVLIYWLIRFVFEFPVIGVPLLLLVLYFWFFRMRHVAD
                     VQRDRWSSDDTFALAPRPTRRPGLEKIRARDPEFSAVLFEDFVYALYARVHTARSDPA
                     AMTALTPYVAEGSRTALLARDPSGVPISGVVVGAMQVLSVKVPGAADSNGKIRVDLKF
                     EANYTATIDGEPHGYYIEERWGLQRSISVQSKPPGEALEFRCPSCGAPFESSGGERCR
                     YCKQEVGGGRFDWTLTHVNLLQQDTRPPALTADVVERGTDAPTVFDPRVQAEHAALIG
                     EDPGAAPELIERRLRAIYSELNIAWSTLDLQRIRPLVSDSMFNYLEYWILAYRVQGLR
                     NRLEEMAISRVQIVKLTRDRHYDAITLRFWASGRDYTVRADDGETVSGSKSRKRSYSE
                     YWTLIRGARVRGAPREPGNCPSCGAGTERVNMAGTCEYCGSHLTRGEFDWVLSKIEQD
                     DSYAG"
     gene            1557..2156
                     /locus_tag="VGB85_11680"
     CDS             1557..2156
                     /locus_tag="VGB85_11680"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEY0134739.1"
                     /translation="MQHSFKRHLRNAFITGIFSAIPIVITAVVVWYVEKLTREPITAL
                     FGVDTPFIGIAAAIVGIYLLGVLVNSLLGRYILRGLDALLRRVPILRDVYEAWRQVIL
                     TPGGKEGIYANVVLVPTSGDGLAMGFTSGEPVEGNPDLCCVFVPGTPNPTAGRLVFAR
                     RDKCINLGISAEEAFKFALSGGNYVPAPVGPLSAARLEP"
     gene            complement(2204..>2869)
                     /locus_tag="VGB85_11685"
     CDS             complement(2204..>2869)
                     /locus_tag="VGB85_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134740.1"
                     /translation="EDNALFGLAVAARLQSGKTARDSWKAWSAGFTPELVLGSEFSKH
                     VTWSFKTDAAMTDGVAVDRWTIEPTPAARKEMEGKMDAEGKAFVDKALGGLVLNIDRA
                     EVAGNVVFTIAPKAEASYMKRTIAAFQGKGNVAAEPGLAKVLARDPSTAGILAIDLKE
                     SMAWIRGLSAYGAKTEHVPQNIGTDLGDFYLTARYTSDGATAMEYVIAQQLIEQLKTL
                     IPN"
BASE COUNT          392 a         1114 c          913 g          450 t
ORIGIN      
        1 gcccgcccgc tcgcgatcct cgcggccatc tcgatcgtcg ccctcgtcgc cctcgacgcc
       61 gcggcgcgcc ccggcggcgg tcacggttac tcgggcggtg gcggtggtgg tcacagcggc
      121 ggcggtggtt acagcggcgg cggtggttac agcggcggca gtcacagcag cagcggcgga
      181 ggcggcgacg ccggcgtcct gatctactgg ctgatccgct tcgtgttcga gttcccggtc
      241 atcggcgtcc cgctgctgct gctggtcctc tacttctggt tcttccgcat gcgtcacgtc
      301 gccgacgtgc agcgcgaccg ctggtcgagc gacgacacct tcgcgctcgc gcctcgcccg
      361 acccgccggc ccggcctcga gaagatccgc gcccgcgacc ccgagttctc cgccgtcctg
      421 ttcgaggact tcgtgtacgc gctctacgcc cgcgttcaca ccgcgcgcag cgaccccgcg
      481 gcgatgaccg cgttgacgcc ctacgtcgcc gagggctcgc gcaccgccct gctcgcgcgc
      541 gacccctccg gcgtgccgat ctccggcgtc gtcgtcggcg cgatgcaggt gctcagcgtc
      601 aaggtccccg gcgcggccga ctccaacgga aagatccgcg tcgacctcaa gttcgaggcc
      661 aactacaccg ccaccatcga cggcgagccg cacggctatt acatcgagga gcgctggggc
      721 ctccagaggt cgatctccgt ccagagcaag ccccccggcg aggcgctcga gttccgctgc
      781 cccagctgcg gcgccccctt cgagtcgagc ggcggcgagc gctgccgcta ctgcaagcag
      841 gaggtcggcg gcggccgctt cgactggacg ctgacccacg tcaatctcct gcagcaggac
      901 acgcgcccgc cggccctgac cgccgatgtc gtcgagcgcg gcaccgacgc gccgaccgtg
      961 ttcgacccgc gcgtgcaggc cgagcacgcc gccctgatcg gcgaggaccc gggcgccgcg
     1021 ccggagctga tcgaacgccg cctgcgcgcg atctacagcg agctcaacat cgcctggagc
     1081 accctcgacc tccagcggat ccgcccgctc gtctccgact cgatgttcaa ctacctcgag
     1141 tactggatcc tcgcctaccg ggtccagggc ctgcgcaacc ggctcgagga gatggcgatc
     1201 agccgcgtgc agatcgtcaa gctgacccgc gaccgccact acgacgcgat caccctgcgc
     1261 ttttgggcct cgggccgcga ctacaccgtg cgcgccgacg acggcgagac ggtgtccggc
     1321 agcaagagcc gcaagcgcag ctacagcgag tactggacgc tgatccgcgg cgcccgggtc
     1381 cgcggcgccc cgcgtgagcc cggcaactgc ccgagctgcg gcgccggcac cgagcgcgtc
     1441 aacatggccg gcacctgcga gtactgcggc tcgcacctga cccgcggcga gttcgactgg
     1501 gtcctcagca agatcgagca agacgactcc tacgccggct agactcgcgg gccccgatgc
     1561 agcacagctt caagcgtcac ctgcgaaacg ccttcatcac cggcatcttc tcggcgatcc
     1621 cgatcgtcat caccgccgtc gtcgtctggt acgtcgagaa gctgacccgc gagccgatca
     1681 ccgccctgtt cggcgtcgac acccccttca tcggcatcgc cgccgcgatc gtcgggatct
     1741 acctgctcgg cgtgctcgtc aacagcctgc tcggccgcta catcctgcgc ggcctcgacg
     1801 ccctgctgcg ccgcgtgccg atcctgcgcg acgtctacga ggcctggcgt caggtcatcc
     1861 tcacccccgg cggcaaggag ggcatctacg ccaacgtcgt gctcgtgccg accagcggcg
     1921 atggcctcgc catgggcttc accagcggcg agcccgtcga gggcaacccc gacctgtgct
     1981 gcgtgttcgt gcccggcacc ccgaacccga ccgccggccg cctcgtgttc gcccgccgcg
     2041 acaaatgcat caacctcggc atcagcgccg aggaggcctt caagttcgcg ctgtcgggcg
     2101 gcaactacgt gccggcgccg gtcggcccgc tgtcggccgc gcgcctggag ccctgacgct
     2161 cgcgggcgcg gacacctgcg tgcccgcgcc ggcgtcctga tcctcagttc gggatcagcg
     2221 tcttgagctg ctcgatcagc tgctgcgcga tcacgtactc catcgccgtc gcgccgtcgc
     2281 tggtgtagcg ggccgtcagg tagaagtcgc cgaggtcggt gccgatgttc tgcggcacgt
     2341 gctcggtctt ggcgccgtac gccgacagcc cgcggatcca cgccatcgac tccttgaggt
     2401 cgatcgccag gatgcccgcc gtgctcgggt cgcgcgcgag caccttggcc agccccggct
     2461 cggccgcgac gttgcccttg ccctggaacg ccgcgatcgt gcgcttcatg tagctcgcct
     2521 cggccttcgg ggcgatcgtg aagaccacgt tgcccgcgac ctcggcgcgg tcgatgttga
     2581 ggaccagacc gccgagcgcc ttgtcgacga aggccttgcc ctccgcgtcc atcttgccct
     2641 ccatctcctt cctcgccgcc ggcgtcggct cgatcgtcca gcggtcgacc gccacgccgt
     2701 cggtcatcgc cgcgtcggtc ttgaagctcc acgtcacgtg cttcgagaac tccgagccga
     2761 gcaccagctc cggcgtgaac ccggccgacc acgccttcca cgagtcgcgc gccgtcttgc
     2821 cgctctgcaa ccgcgccgcg accgcgaggc cgaacagcgc gttgtcctc
//
LOCUS       DASWNJ010000699         1797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_82465, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000699 DASWNJ010000000
VERSION     DASWNJ010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82465"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1797
                     /locus_tag="VGB85_11690"
     CDS             <1..>1797
                     /locus_tag="VGB85_11690"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY0134741.1"
                     /translation="KTGNVNLILKAIKSGQIECLYNTKTERVEPGVLHLKTPEGKRAY
                     PCDRVIARLGGILPTAFLKDCGIKVAEGSGPTFPEVSEQYETNVPGLYAIGAIVGYPL
                     IKQCMNQGYEVVQAIAGQPVAPADEPLLQEKLLGLPGRPTVSVSLDQIKSQVRLFRSL
                     TTLQLREFLLDSDTHVMLAGETVYRHHEFGDTLFLVVAGTVEIEQQASAAGAATRTIL
                     RREGDFFGEAGLIANRPRAGTARAKTDCVVIELVRRAALKLLASVPSAREEFERITIV
                     RQLQDDLLSDLTEADLAEVVAKAEIRSFKPGDRLIEEGDEKDLAAFLIRSGSVTISRK
                     VDGKESVLAYEPPGRLVGEMALLRQGPRAASVTAAIQTDAIRIDGDVFRRLVDGRPDL
                     RAKIDELVRNRMLGGATGGAAAAGNFAPGLLEKGRGGLGEATDVLLIDESLCIRCDNC
                     EKACAESHDGISRLDREAGPTFAMLHVPTSCRHCQHPHCMKDCPPAAIHRGAGGEVWI
                     DDTCIGCGNCVENCPYGVIQLAEPPPPKPGLLQWLLFGRGPGPGEDKALKAKKKAKPP
                     AEGAKKLAVKCDMCAGIRGGAACVRACPTGAAI"
BASE COUNT          290 a          631 c          647 g          229 t
ORIGIN      
        1 aagacgggca acgtcaacct gatcttgaag gccatcaaga gcggccagat cgagtgcctc
       61 tacaacacca agaccgagcg ggtcgagccg ggcgtgctcc atctcaagac gcctgagggc
      121 aagcgcgcct acccctgcga ccgggtgatc gcccggctgg gcggcatcct gccgacggcc
      181 ttcctcaagg attgcgggat caaggtcgcc gagggcagcg gcccgacctt ccccgaggtc
      241 agcgagcagt acgagacgaa cgtgccgggc ctctacgcga tcggcgccat cgtgggatac
      301 ccgctgatca agcagtgcat gaaccagggc tacgaggtgg tccaggcgat cgccggccag
      361 cccgtggcgc ccgccgacga gccgctgctc caggagaagc tgctgggtct gccgggccgg
      421 ccgacggtga gcgtctcgct cgaccagatc aagtcgcagg tgcgcctgtt ccggagcctg
      481 accacgctcc agctgcgcga gttcctgctg gatagcgaca cgcacgtgat gctcgcgggc
      541 gagacggtct accggcacca cgagttcggg gacacgctgt tcctcgtcgt cgcgggtacg
      601 gtcgagatcg aacagcaggc gtcggccgcg ggcgccgcga cccgcacgat cctgcgccgc
      661 gagggcgact tcttcggcga ggccgggctg atcgcgaacc ggccgcgcgc cggcaccgcc
      721 cgggcgaaga ccgactgcgt ggtgatcgag ctggtgcggc gcgcggcgct caagctgctg
      781 gcctcggtgc cgagcgcccg cgaggagttc gagaggatca ccatcgtccg gcagctccag
      841 gacgacctgc tgtcagacct caccgaggcg gacctcgcgg aggtggtcgc gaaggcggag
      901 atcaggagct tcaagccggg cgaccggctc atcgaggagg gcgacgagaa ggacctcgcc
      961 gcgttcctga tccgctccgg ctcggtcacc atctcccgca aggtcgacgg caaggagagc
     1021 gtgctcgcct acgagccccc gggccggctc gtgggcgaga tggcgctgct gcgccagggg
     1081 ccgcgcgccg ccagcgtcac cgcggcgatc cagaccgacg ccatccgcat cgacggcgac
     1141 gtcttccgcc ggctcgtcga cggccggccg gatctcaggg ccaagatcga cgaactcgtc
     1201 cggaaccgga tgctcggcgg cgccacgggg ggtgcggccg ccgccggcaa cttcgccccg
     1261 ggcctgctgg agaagggccg cggcggcctg ggcgaagcca ccgacgtcct gctgatcgac
     1321 gagtcgctct gcatccgctg cgacaactgc gagaaggcct gcgccgagag ccacgacggg
     1381 atctcgcggc tcgaccgcga ggccggcccg accttcgcga tgctgcacgt gcccacctcc
     1441 tgccggcact gccagcaccc gcactgcatg aaggactgcc cgcccgccgc gatccaccgc
     1501 ggtgctggcg gcgaggtctg gatcgacgac acctgcatcg gctgcggcaa ctgcgtcgag
     1561 aactgcccct acggcgtgat ccagctcgcc gagccgccgc cgccgaagcc cgggctgctc
     1621 cagtggctct tgttcggccg cgggcccggg ccgggcgagg acaaggcgct caaggccaag
     1681 aagaaggcga agccgccggc cgagggggcg aagaagctcg cggtgaagtg cgacatgtgc
     1741 gcggggatca ggggcggggc cgcctgcgtg cgcgcctgcc cgaccggggc ggcgatc
//
LOCUS       DASWNJ010000700         1789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_189658, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000700 DASWNJ010000000
VERSION     DASWNJ010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189658"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..>1789
                     /locus_tag="VGB85_11695"
     CDS             157..>1789
                     /locus_tag="VGB85_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134742.1"
                     /translation="MHTSRSSVRVVTGLALALSGCGDTPAGTAETGTGSTGTSSSTGT
                     TDVPTTDVPTGTTTGEPTTTTTTTTATMTTVDTTGEPTTTEPGTTGDVCPMPLCGDPP
                     VCCLEGEKCMAGACVPGCGSSGGLEFLDVEIEWSWTTNEVTVMPLVADIQGDAVPEVV
                     VNAMRVDNITRELGEIVVLDGKTGTEIWRISEDPQNDKFGSLSLSTPVLADVSGDGRP
                     DIIYPGRGPGTPSTIQSLVHAVDGDGKLLWTGHTADDMPVKVRWVHGAAVATNFDDDP
                     EAEIAIGGAIFDHDGLLVWNQDNKSGLLGTPSDNKNPPSPIYVGGLATAVDLTGDGKP
                     ELLTGREAWTVDWTAGAPPTVSLTMLWQNTSGKGNDGWPAVADLDKNGTPEVVLVAWP
                     DIKVLDGATGKLWCGVDPTGVMCDADDTLRTQPIAIEGGNLGGPATIADLDGDGRPEV
                     GIAGGTAFAVYDFNRMGEMIVKPDNDPLPAAGAMYVRWSQPTQDNSSAATGSSVFDFQ
                     GDGAAEVAYQDECRVHVYDGKSGDPKLAINNSSGTV"
BASE COUNT          312 a          683 c          565 g          229 t
ORIGIN      
        1 cacacctatc atgtcaccga cgccgactcg accggcagcc ccccgctgtt cgagcaggcc
       61 aactggctcg accccggcct caacaacttt cgtcagaacg tgcagggcga gtgcaagtag
      121 gtgacaaacc accggccccg cgtctaaggt acccccatgc atacttcgcg ctcctccgtt
      181 cgggtcgtca ccggcctcgc gctggcactc tctggctgcg gtgacacccc cgccggcacc
      241 gccgagaccg gcaccggcag caccggcacg tcgtcctcca cgggcaccac ggatgtcccg
      301 accacggacg tcccgaccgg cacgacgacc ggcgagccga cgaccaccac caccacgacc
      361 accgccacga tgacgaccgt cgacaccacc ggcgagccga cgaccaccga gcccggcacc
      421 accggcgatg tgtgtccgat gccgctgtgc ggcgatcctc ccgtgtgctg cctcgagggc
      481 gagaagtgca tggcgggcgc ctgcgtgccc ggctgcggct cctcgggcgg gctcgagttc
      541 ctcgacgtcg agatcgagtg gtcgtggacg accaacgagg tcaccgtcat gccgctcgtc
      601 gccgacatcc agggcgacgc cgtgccggag gtcgtcgtca acgccatgcg ggtcgacaac
      661 atcacccgcg agctcggcga gatcgtcgtg ctcgacggca agaccgggac cgagatctgg
      721 cggatctccg aggacccgca gaacgacaag ttcggctcgc tcagtctatc gacgcccgtg
      781 ctcgccgacg tcagcggcga cgggcgcccc gacatcatct accccggtcg cggccccggc
      841 acccccagca cgatccagag cctcgtgcac gccgtcgacg gggacggcaa gctgctgtgg
      901 accggccaca ccgccgacga tatgccggtc aaggtccgct gggtgcatgg cgccgcggtt
      961 gcgaccaact tcgacgacga ccccgaggcc gagatcgcga tcggcggcgc gatcttcgac
     1021 cacgatggcc tgctcgtgtg gaaccaggac aacaagagcg gcctgctcgg cacgccgagc
     1081 gacaacaaga accctccgtc ccccatctac gtcggcggcc tcgccaccgc cgtcgacctc
     1141 accggcgacg gcaagcccga gctgctgacc ggccgcgagg cgtggaccgt cgactggacc
     1201 gccggtgcgc cgccgaccgt gtcgctgacc atgttgtggc agaacaccag cggcaagggc
     1261 aacgacggct ggccggccgt cgccgacctc gacaagaacg gcacccccga ggtcgtgttg
     1321 gtcgcctggc ccgacatcaa ggtgctcgac ggcgcgaccg gcaagctatg gtgcggcgtc
     1381 gacccgaccg gcgtgatgtg cgacgccgac gacaccctgc gcacccagcc gatcgcgatc
     1441 gaaggcggca acctcggcgg tccggcgacg atcgccgacc tcgacggcga cggccggccc
     1501 gaggtcggca tcgccggcgg caccgccttc gccgtctacg acttcaatcg catgggcgaa
     1561 atgatcgtca agcccgacaa cgacccgctg cccgccgccg gcgcgatgta tgtgcgatgg
     1621 tcgcagccga cccaggacaa ctcctcggcc gcgaccggat cctcggtgtt cgacttccag
     1681 ggcgacggcg ccgccgaggt cgcctaccag gacgagtgcc gcgtccacgt ctacgacggc
     1741 aagtccggcg accccaagct cgcgatcaac aactcgagcg gcaccgtcc
//
LOCUS       DASWNJ010000701         3974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_648577, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000701 DASWNJ010000000
VERSION     DASWNJ010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648577"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            298..1248
                     /gene="panB"
                     /locus_tag="VGB85_11700"
     CDS             298..1248
                     /gene="panB"
                     /locus_tag="VGB85_11700"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEY0134743.1"
                     /translation="MSTSPNHRGPGEIPGAAAATAVQVAAAPAPQPRRITVPQLGRFK
                     RDGQAITMVTAYDATFARLVDEAGVDVILVGDSLGMVMQGHPHTLEVTLEHMAYHARC
                     VRRAVARAHLVVDMPFMSYQVSPEQALVSAGRLVQEGGAEAVKLEGGARCVDAVRKIV
                     SAGIPVMGHVGLTPQSVHEFGGFRVQARGQDAAERLRQDSLALQEAGAYALVLEGIPA
                     ALARQVSESLAIPTIGIGAGAGCDGQVLVMQDLLGLDDRFKPKFVKRYAELAGLVREA
                     FSEFARDVRERRFPGDEHSFAGEARVEPVVEPGSGAYGPT"
     gene            1262..2104
                     /gene="panC"
                     /locus_tag="VGB85_11705"
     CDS             1262..2104
                     /gene="panC"
                     /locus_tag="VGB85_11705"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY0134744.1"
                     /translation="MKVLRTREELLAWRASLPAEMPVAFVPTMGYLHEGHLSLLREGR
                     RRADGGALVLSIFVNPTQFGPKEDLDKYPRDEAGDLAKAQTCGVDVAFCPTDARALYP
                     NGTSTWVEVEGLDKHLCGASRPGHFRGVCTVVAKLWGLVRPSLGLFGEKDYQQLAILR
                     RMHADLNFVGEVVGFPIVREPDGLAMSSRNANLGPQARAEALAISRFLNGVRERFAGG
                     VRDSAKLLADFATQLAPGRVDYVSVVDADDLQPIEVVTRPALVAVAAFFGGVRLIDNT
                     VLRP"
     gene            complement(2111..2725)
                     /locus_tag="VGB85_11710"
     CDS             complement(2111..2725)
                     /locus_tag="VGB85_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134745.1"
                     /translation="MIALRGLYRGLEALYGADSGVDPLDYLVPFRNRGEHSELVLVRE
                     SEDGVEIGLALAEGALESLAGRPHDSVLGDAALADTLPVVEGLSHLLYLVEAVRRERP
                     VSGLELETQAEVDKLALCLLDRWPARRQEFARLVDRIFCQWSLLPGLDADMRERYQQA
                     NRVALGFVKRLERTVRAGQLGGLRRALRRFWAADMSGKQALAGA"
     gene            complement(2722..3294)
                     /locus_tag="VGB85_11715"
     CDS             complement(2722..3294)
                     /locus_tag="VGB85_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134746.1"
                     /translation="MTAKLAFDGSLEAFFFDQVGEAQTRAGATLPREVEAYVVHLLAA
                     YVQKPAVAGRRTGPLALDYLAARAQEGSARAQALRQVGDRALFIAGVVPHSLDRTPVK
                     LSYVRSLGSTAYRQVSDGHGALAVFHELANHFEVAAEVISEATDGRGDADRDLLGLYD
                     RWRRYGEDRDARRLLAAGIVLGREGSDTLQ"
     gene            3653..3949
                     /gene="groES"
                     /locus_tag="VGB85_11720"
     CDS             3653..3949
                     /gene="groES"
                     /locus_tag="VGB85_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020055847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY0134747.1"
                     /translation="MSTKVRPLNDRVLIKRMAEEEKTAGGLFIPDAAKEKPARGIVIA
                     VGQGKSDDTGKRTPLDVKAGDKVLFGKYSGTEIKLDGEDHMILREEEILAVLEG"
BASE COUNT          610 a         1304 c         1436 g          624 t
ORIGIN      
        1 agcgtgacgc agacgcgcgg cgcggcggag cgcaggtgcg tggctgttcg acgttccgcg
       61 agatctgcgg ccctcatggc aaagctgaca attcggaccc atcgttgtcc acaatgccgg
      121 gcgcggtgtc ggaagccaac atacaaagct ttcttgcgcc gcaaacgcgg tgatttttcg
      181 caccggttcg cgatcggacg tcggggtgag cgggatcgat tgtccggatc tcgaagggcg
      241 attcgggctt ttgcgggcgc gcttcggtgc ctttgagata tgctccgcgc cgcccgcatg
      301 tcgacttcgc ccaaccatcg agggcccgga gagatccccg gggctgctgc cgcgaccgct
      361 gtccaggtgg ccgccgcccc ggcgcctcag ccgcgccgga tcaccgttcc gcagctcggg
      421 cgcttcaagc gcgatgggca ggcgatcacg atggtcaccg cctacgacgc gaccttcgca
      481 aggctggtcg acgaggccgg cgtcgacgtg atcctcgtcg gcgacagcct cgggatggtg
      541 atgcagggcc acccgcacac gctcgaggtg acgctcgagc acatggccta tcacgcccgc
      601 tgcgtgcgcc gggcggtcgc gcgggcccac ctcgtcgtcg acatgccgtt catgagctac
      661 caggtcagcc cggagcaggc gctggtgtcc gcggggcggc tggtgcagga gggcggcgcc
      721 gaggcggtca agctcgaggg cggggcccgc tgcgtcgacg cggtgcgaaa gatcgtgtcc
      781 gcggggatcc cggtgatggg gcacgtcggt ctgaccccgc agagcgtgca cgagtttggt
      841 ggctttcgtg tgcaggcccg ggggcaagac gccgcggagc ggctgcgcca ggactccctc
      901 gcgttgcaag aggccggcgc ctacgccctc gtgctcgagg ggatccccgc cgcgctcgcc
      961 cgccaggtca gtgagtcgct agcgatcccg acgatcggga tcggtgccgg cgccggctgt
     1021 gacggccagg tgctcgtcat gcaggacctg ctcggcctcg acgaccgctt caagcccaag
     1081 ttcgtaaaac gctacgcgga gctcgccggg ctggtccgcg aggctttttc agagttcgcc
     1141 cgcgatgttc gcgagcgccg cttccccggc gacgagcaca gcttcgccgg cgaggcccgc
     1201 gtcgagccgg tcgtcgagcc aggctccggc gcgtacgggc cgacctgaag gacctggccc
     1261 gatgaaggtg ctgcgcacgc gtgaggagct gctggcctgg cgagcgtcgc tgcccgccga
     1321 gatgccggtc gcgttcgtgc cgacgatggg ctacctgcac gagggtcacc tgtcgctgct
     1381 gcgtgagggc cggcgacgcg ccgacggtgg cgcgctggtg ctgtcgatct tcgtcaaccc
     1441 gacgcagttc gggccgaagg aggatctcga taaatacccg cgcgacgagg ctggtgatct
     1501 cgcgaaggca cagacatgcg gggtcgatgt cgcgttctgc ccgactgacg cgcgcgcgct
     1561 gtatcccaac ggcacgagca cctgggtcga ggtcgagggg cttgataagc atttatgtgg
     1621 tgcgtcgcgc ccgggccact ttcgcggggt gtgcacggtg gtcgcgaagc tgtggggatt
     1681 ggtccgaccg agcctgggtt tgttcggcga gaaggactat caacagctgg cgatccttcg
     1741 ccgcatgcac gccgacctca attttgtcgg cgaggtcgtc ggtttcccga tcgtgcgcga
     1801 gccggacggt ctggcgatgt cgagccgcaa cgccaacctc ggcccgcagg cgcgcgccga
     1861 ggcgctggcg atctcgcggt ttttgaatgg ggttcgggag cgcttcgcgg gtggggtgcg
     1921 ggactcggct aaattgctcg cggacttcgc cacgcagctc gcgccgggtc gcgtcgacta
     1981 cgtgtcggtc gtcgacgccg acgacctgca gccgatcgag gtggtgacga ggccggcgct
     2041 cgtcgccgtc gcggccttct tcggcggcgt gcggctgatc gacaacaccg tgctgcggcc
     2101 gtgaaaaata tcaggcgccg gcgagcgctt gcttgccgga catgtcggcg gcccagaacc
     2161 ggcgcagggc ccggcgcagg ccgccgagct ggccggcgcg gacggtccgc tcgaggcgct
     2221 tcacgaaacc cagcgcgacg cggttggcct gctggtatcg ctcgcgcatg tcggcgtcga
     2281 ggccgggcag caggctccac tgacagaaga tgcggtcgac caggcgggcg aactcctggc
     2341 ggcgcgcggg ccagcggtcg agcaggcaca gcgcgagctt gtcgacctcg gcctgggtct
     2401 cgagctcgag gccgctgacg ggtcgttcgc ggcggacggc ctcgacgaga tagaggaggt
     2461 ggctgaggcc ctctacgacc gggagggtgt cggcgagggc ggcgtcgccg agcacgctgt
     2521 cgtggggtcg gccggcgagg ctctcgagcg cgccctcggc gagggcgagc ccgatctcga
     2581 cgccgtcctc ggactcgcgg accaggacca gctcgctgtg ctcaccccgg tttctgaagg
     2641 ggacgaggta gtcgagcggg tcgacgccgg agtcggcgcc atagagggcc tcgaggccgc
     2701 gatagaggcc gcgcagggcg atcattgcag ggtgtccgag ccctcgcggc cgagcacgat
     2761 gcccgcggcg agcagtctgc gggcgtcgcg gtcctccccg tagcgacgcc agcgatcgta
     2821 gaggccgagc aggtcgcggt ccgcgtcacc ccggccgtcg gtcgcctcgg agatcacctc
     2881 ggcggcgacc tcgaagtggt tcgccagctc gtggaacacc gcgagcgcgc cgtggccgtc
     2941 cgagacctgg cggtaggcgg tcgaccccag cgagcgcacg tagctgagct tcaccggcgt
     3001 gcggtcgagg ctgtgcggca cgaccccggc gatgaacagt gcgcggtcgc cgacctggcg
     3061 cagcgcctgg gcccgcgcgc tgccctcctg ggcgcgggcc gccaaatagt cgagcgccag
     3121 cggacccgtg cgccggccgg cgacggccgg cttctgcacg taggcggcga gcaggtggac
     3181 gacgtaggcc tcgacctcgc gggggagtgt ggcccccgcc cgggtctggg cctcgccgac
     3241 ctggtcaaag aagaaggctt cgagcgaacc atcgaacgcg agcttggccg tcatcagcga
     3301 gtccccctgc ttgccaagct agcaggggtc ggcgaagcga caacgaagtt atgtgagcaa
     3361 accgggcaga gctgcgccaa agcgcccgga caaaattgtc agtaccgggg gttgaccccc
     3421 cgcggggtgt cactacactc gcgccacatt agcagtcacc ccagggcgac tgctagcaat
     3481 gtcccccctc ggcccgctaa accggctccg acgggtttcc cccatttacg cgagcgtcgc
     3541 gcctaccgcg cggttgcctc gttccgggcg aatgcgccca aaaacttcat ggcctccatc
     3601 actcatgggg ccctgatttc gctgttccaa cgagtacaca ggagactgct acatgtcgac
     3661 gaaggttcgt ccgctgaacg atcgcgttct tatcaagcgt atggccgagg aagagaagac
     3721 cgctggcggt cttttcatcc ccgatgccgc caaagagaag ccggcacgcg gcatcgtcat
     3781 cgcggtgggt cagggcaagt cggatgacac cggcaagcgc accccgctcg acgtcaaggc
     3841 cggggacaag gtcctgttcg gcaagtactc cggcaccgag atcaagctcg acggagagga
     3901 tcacatgatc ctgcgcgagg aagagatcct cgcggtgctc gaaggctagt tcctgcgccc
     3961 gcaggacgcg gatc
//
LOCUS       DASWNJ010000702         1670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_682955, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000702 DASWNJ010000000
VERSION     DASWNJ010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682955"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VGB85_11725"
     CDS             <1..272
                     /locus_tag="VGB85_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134748.1"
                     /translation="SVSSCGGYDDWFSRKSYRAQVGYGLTVFSQMLGVTPQFLRFDVA
                     VPLIRRETLCLGHIHPDYLGEIQGLAPGEYTLPPVGLNLTFLQPF"
     gene            351..1091
                     /locus_tag="VGB85_11730"
     CDS             351..1091
                     /locus_tag="VGB85_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134749.1"
                     /translation="MSTTSIAADAPGDLDRLYTYAAYMLGDRAAALAAVRSMLAGTSP
                     GPLLTRLQAVRTTILAHAARRKQSPVDVRTRLDDTLRLGTSLSMKMGPTALRSGVRRL
                     PVLLTGFMQTCLIAAVQALPPMQREAFVLLVVLGLPEADVIALQGKTVHGFSSVKTKM
                     FRSMNAYLAPRCAHIHPNNPCKCPNRLQGALDQNFVQLPEHEVASEAYPTGVYPDVRQ
                     LYGAVPPLRLGAEVLGSLRGGAVVHMVD"
BASE COUNT          240 a          577 c          579 g          274 t
ORIGIN      
        1 ccagcgtgtc gtcgtgcggc ggctacgacg actggttctc gcgcaagagc taccgggcgc
       61 aggtcggcta cggcctgacg gtgttctcgc agatgctcgg cgtgaccccc cagttcctgc
      121 gcttcgacgt cgcggtgccg ctgatccgcc gcgagacgct gtgcctcggc cacatccacc
      181 cggactacct cggcgagatc cagggcctcg cgcccggcga gtacacgctg ccgccggtcg
      241 gcctcaacct gacgttcctg cagccgttct aaaggtcgcg ctcgactccg cgcctgcgag
      301 gtttcggccc gcggctggtc cgcttcgtgc gtcactgcta cgttgccgcc atgtcgacga
      361 cgtcgatcgc cgcggacgcg cccggggacc tcgatcgcct gtacacctac gccgcctata
      421 tgctcggtga ccgcgcggcc gccctggccg ccgtgcgcag catgctcgcc ggcaccagcc
      481 ccggccccct gctgacccgc ctgcaggcgg tgcgcaccac gatcctcgcg cacgcggccc
      541 gccgcaagca gagcccggtc gacgtgcgca cccgcctcga cgacacgctg cgcctcggca
      601 ccagcctgtc gatgaagatg ggcccgacgg cgctgcgcag cggcgtgcgc cgcctcccgg
      661 tgctgctgac cggcttcatg caaacctgcc tgatcgcggc cgtgcaggcg ctgccgccga
      721 tgcagcgcga ggccttcgtg ctgctcgtcg tgctcggctt gcccgaggcg gacgtgatcg
      781 cgctgcaggg caagacggtg cacggcttct cgagcgtcaa gacgaagatg ttccggtcga
      841 tgaacgccta cctcgcgccg cgctgcgcgc atatccaccc gaacaacccc tgtaaatgcc
      901 ccaaccggct gcagggggcg ctcgaccaga acttcgtgca attgcccgaa catgaggtgg
      961 ccagcgaggc gtatccgacc ggggtgtacc cggacgtgcg ccagctgtat ggcgcggtgc
     1021 ctccgctgcg gcttggggcg gaggtacttg gctcgctgcg tggtggggcc gttgtgcata
     1081 tggtggatta gggggcgctc cggctgcggc gcggaggacg tcgtacggcc ggtatcgggc
     1141 gggcgcggag gacgtcgcac ggccggggcg ggtccggctg ggggcgcgcg cattgtctag
     1201 agccggatgt ccgccggggg tttcgccctc aaacacgacg cgcgtttcga ggcgtgtccg
     1261 aagaccaggg gcccgtcgga ctcggtcgaa acccccgtcg gcccaccggc acccacactg
     1321 ctctaaaggc cggtcggtcg gcgcagccga gagccggacc cgccccggcc tgagcatacg
     1381 atatggccgc gcggacatgt ccacagggac atgttctccc ggacagacac acgggatacg
     1441 cggaggcctc tgtgccctcg acggtggatt gtcgaggtcg cgttatgagg cgcatcgcgc
     1501 ggtcgcgtcc gatgattcat cagccaagcc atggcggacc tcggcgcggg cggggaatat
     1561 gtggagttga tggagacttc ctattgcggg cgagagcctt cattatcaaa agtatgtccc
     1621 gggagacatg tccgcgggac caggttgttt ggtcagggcc gacgcgggtc
//
LOCUS       DASWNJ010000703         2139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_325908, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000703 DASWNJ010000000
VERSION     DASWNJ010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325908"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VGB85_11735"
     CDS             complement(<1..692)
                     /locus_tag="VGB85_11735"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0134750.1"
                     /translation="MSSTEWSAARGEKWRAGLCAMEAMLLPTQEPLLRALQLDAPLRI
                     ADVGCGGGGTTLEISRRAPAGSVVDGLDISPALIELARARPRPDGRAVAFEVADLATA
                     TPRRPYDRLASRFGVMFFAEPDAAFANLARWLAPGGRLAFAVWGALSDNPWMSSVREV
                     VAHFIDLPAIDPGAPGPFRYADPDELLAALARAGFASLKVDDWRGALPVGSSPAEAAS
                     FALSSFASFGELL"
     gene            complement(700..1206)
                     /locus_tag="VGB85_11740"
     CDS             complement(700..1206)
                     /locus_tag="VGB85_11740"
                     /inference="COORDINATES: protein motif:HMM:NF022606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2938 domain-containing protein"
                     /protein_id="HEY0134751.1"
                     /translation="MIQPVEFMLRTLAIGAGATLLMDAWALLLRQFGVPSLDFALLGR
                     WVGHLRHGLWMHEKIARAAPVRGELVLGWCTHYAIGIGFAGLLLATIGLKWARSPSLW
                     PALVIGIVTVVAPLFILQPALGAGIASANTPTPLFNCVKSVVTHTCYGVGLYLAALVT
                     AAIFPAGE"
     gene            1340..>2139
                     /locus_tag="VGB85_11745"
     CDS             1340..>2139
                     /locus_tag="VGB85_11745"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0134752.1"
                     /translation="MRVGVIGTHWGRMHIGGFRGAGAEIAALCGRDLEATRRACAEEG
                     VALATTDVGALCRAVDLVVVASPDRVHAAHVRAAIAAGCHVLCEKPLTRGVADAAALV
                     ELTAASDRTHAVNFPYRMLPPLRTLADWLRTRGPARATQVRVRNSFLSAGTGETDDDD
                     AMLRASGDLGGLSHVIDAALWLLDARPRDVQATLRGRPVGDAGLLLRLDGGGTLAIAH
                     VASVVPGIWGDWHIVGDDYEVRLAGGYVPALAGWQLGPVEVAGRDGTW"
BASE COUNT          315 a          744 c          814 g          266 t
ORIGIN      
        1 agcagctcgc cgaacgacgc gaacgacgat aacgcgaagc tcgcggcctc ggccggcgag
       61 ctgccgaccg gcagtgctcc tcgccagtcg tcaactttga ggctggcaaa gccggctcgc
      121 gcgagcgcgg cgagcagctc gtcggggtcc gcgtagcgaa acggcccggg cgctccggga
      181 tcgatcgccg gcagatcgat gaaatgcgcg accacctcgc gcacggatga catccagggg
      241 ttgtcggaca gcgcgcccca gaccgcgaac gcgaggcgac cgcccggcgc gagccagcgc
      301 gccaggttgg cgaaggcggc gtcgggctcg gcgaagaaca tgacgccgaa gcgcgaggcc
      361 agccggtcgt acggccgccg cggcgtcgcc gtggcgaggt cggcgacctc gaaggcgacc
      421 gcgcgcccgt cgggccgcgg gcgcgcgcgg gcgagctcga tgagggccgg cgagatatcg
      481 aggccatcaa cgacgctgcc ggccggcgcc cgccgcgaga tctcgagcgt cgtcccgccg
      541 ccgccgcagc cgacatcggc gatcctgagc ggcgcgtcga gctgcagcgc gcgcagcagc
      601 ggctcctgtg tcggtaataa catcgcttcc atggcacaga ggccggcgcg ccacttctcg
      661 ccgcgcgcgg ccgaccattc ggtggagctc atgttcatat cactctccgg cggggaagat
      721 cgcggccgtg acgagcgccg cgagatacag accgacgcca taacaggtgt gcgtgaccac
      781 gctcttgacg cagttgaaca gcggcgtcgg cgtattcgcc gaggcgatgc cggcccccag
      841 cgcgggttgg aggatgaaca ggggcgcgac gaccgtgacg atcccgatca ccagcgcggg
      901 ccacagggaa ggcgagcgcg cccacttcag gccgatggtg gcgagcagga ggccggcgaa
      961 gccgatcccg atcgcatagt gtgtacacca gccgagcacc agctcgccgc ggaccggggc
     1021 cgcacgggcg atcttctcgt gcatccaaag tccgtggcgc aggtggccga cccaccgccc
     1081 cagcagcgcg aagtcgaggg agggcacgcc gaactggcgc agcagcagcg cccaggcgtc
     1141 catcagcaag gtggcgccgg cgccgatcgc cagcgtgcgc agcatgaatt cgacgggttg
     1201 gatcacggtg agctccgatc tccggggaga ctgtccccgg gccgcgcgcg cgtccaatcg
     1261 tagttccgga gggcagacct cggcggcgcc gagggtcgag gtgttcgcgg ccgcacggct
     1321 ctggtaccgt gcgccggcca tgcgcgtggg ggtcatcggg acgcactggg gccggatgca
     1381 tatcggcggg tttcgtggcg cgggcgccga gatcgccgcg ctgtgcgggc gcgacctcga
     1441 ggcgacgcgc cgcgcgtgcg ccgaggaggg cgtggcgctc gcaacgaccg atgtcggcgc
     1501 gctgtgccgg gcggtcgatc tcgtcgtcgt ggcgagcccc gatcgcgtcc acgcggcgca
     1561 cgtgcgggcc gcgatcgcgg ccggctgcca cgtgctgtgc gagaagccgc tgacgcgcgg
     1621 cgtggccgat gcagcggcgc tggtcgagct caccgcggcg agcgatcgca cccacgcggt
     1681 caactttccc tatcgcatgt tgccgccgct gcgcacgctg gccgactggc tgcgcacccg
     1741 cgggcccgcg cgcgcgaccc aggtccgcgt gcgcaacagc ttcctgtccg cggggacagg
     1801 tgagaccgac gacgacgacg cgatgctcag ggcatcgggc gacctcggcg ggctgtcaca
     1861 cgtgatcgac gccgcgctgt ggctgctcga cgctcgcccg cgcgacgtcc aggcgacctt
     1921 gcgcggccgg cccgtcggcg acgccggcct gctgctgcgg ctcgacggcg gcggcacgct
     1981 ggcgatcgca cacgtcgcca gcgtggtccc ggggatctgg ggcgactggc acatcgtcgg
     2041 cgacgattac gaggtgcgcc tggcgggcgg gtacgtgccg gcgctcgcgg gctggcagct
     2101 cggcccggtc gaggtcgcgg gtcgggacgg cacgtggca
//
LOCUS       DASWNJ010000704         2761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_118609, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000704 DASWNJ010000000
VERSION     DASWNJ010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118609"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VGB85_11750"
     CDS             complement(<1..923)
                     /locus_tag="VGB85_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY0134753.1"
                     /translation="MDDATPAPRKLSRLAIASCVVGGLGLAAVALSPGADQPGDSGKY
                     LMVVGGLAILLGIAGRLEIYAKEPAYGGGLWALAGTLVGLGALVVGFMMHFAVGATHG
                     RPLRLRGRVAAAPTTTNEAWIEGAAPDLEGLTPALRERLGKEWLQDAREEHASIPAFS
                     LLSLHLTRLAAPPELVVASHHAALDEVTHARRCFALASAYLGRPCGPAGWAALATASQ
                     EVDLATLACEALADGCVGEGTAAEVAAAAAAQARDPVVRAVLVDIARDEARHAALAWD
                     VLAWALAAGGEAVAQAVSARARALPVAAVDAG"
     gene            1117..>2761
                     /locus_tag="VGB85_11755"
     CDS             1117..>2761
                     /locus_tag="VGB85_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134754.1"
                     /translation="MILDPQALRLAQWFAGLQERLGQLVEDRLHGACALQLEQVSHTL
                     EGAAEVVDQPAPPQSPITLPPGTTTSLLGPLDDDASTTSPDADPSDEDRFLAFLGPAP
                     LRSMTPTLNPPERSEGRGWKSALEHAIRPALHRARGTLRAAAASFTDGTALDSLRAEV
                     EATLVRQPEQLSFDIHPEDSSVVAQDVPPTRVEVPMRRWLLETLKPPLLATISEFGAR
                     IAAAVKGATTELERIETVLDYHLFIVEDGRVAEERDESARAGLGHGVRLVQQLAARLN
                     HEVARTYRWFIAESSMHMDDALAPLRAHRPEEITQTLTALAQRRTTPWWKHTGLGRLR
                     RGAIDVYGKAAPLVREVVRDVRGLLRGDQPRLGYWDLAIASDLAPDDRLPALYRRLFG
                     EIPLGLADLYQPRPALEAICRRVFETWLSGQPRRAEQRGALLVTGDRGAGKRTLVNRV
                     RAELHGELPVRWLPLGPHVAGEARLSAAISEATGAPPASNFGELARTLRLLDQRQAIV
                     LENAEQLFFRTPEGLAQVQSFLDLVRATDDAILWIVLMVQ"
BASE COUNT          373 a         1132 c          922 g          334 t
ORIGIN      
        1 cccgcgtcga cggccgcgac cggcagggcc cgcgcgcggg ccgacaccgc ctgcgccacg
       61 gcctctccac cggccgcgag ggcccacgcg agcacgtccc acgcgagcgc cgcgtgccgg
      121 gcctcgtcgc gggcgatgtc gacgagcacc gcgcggacca ccgggtcacg cgcctgcgca
      181 gccgcggccg cggcgacctc ggcggccgta ccctcgccga cgcagccgtc cgccagcgcc
      241 tcgcacgcga gcgtcgccag gtcgacctcc tggctcgcgg ttgcgagggc ggcccacccg
      301 gcgggcccgc acgggcggcc gaggtacgcg ctggccagcg cgaagcagcg gcgcgcgtgc
      361 gtgacctcgt cgagcgccgc gtggtggctc gccacgacca gctcgggcgg ggccgccagc
      421 cgcgtcaagt gcagcgacag cagcgagaac gccgggatcg acgcgtgctc ctcgcgcgcg
      481 tcctgcaacc actctttacc gagccgctcg cgcagcgcgg gcgtcagtcc ctcgaggtcc
      541 ggcgcggcac cttcgatcca cgcctcgttc gtggtcgtcg gcgccgcggc gacgcgccca
      601 cgcaggcgca acggacggcc gtgcgtcgcc ccgaccgcga agtgcatcat gaacccgaca
      661 accagggcgc cgaggccgac gagcgtgccc gcgagggccc acagcccgcc tccgtacgcg
      721 ggctccttcg cgtagatctc gaggcggccg gcgatcccga gcaggatcgc gaggccaccg
      781 accaccatca ggtatttccc cgagtcaccc ggctggtccg ccccgggaga gagggcgacc
      841 gcggcgagcc cgaggccccc cacgacgcat gaggcgatcg ccagacgaga gagcttgcgg
      901 ggtgcgggcg ttgcgtcatc catcgccggc catcgtagcc ccgggggcgg cacctggctg
      961 cgcgtgagaa cgcgcgcgca acttcgactg tccgaatggc caggtgcaag cgccagagcc
     1021 cgtccacgac cgaacctgtc atgtcgctca tcgcctcggc cccctcgtcg acatgtacgg
     1081 ttcttgcaca tctgtggctc ctgttatgct tcgccggtga tcctcgaccc gcaggccctc
     1141 cgactggccc agtggttcgc tggcctccag gagcggctcg gccagctcgt cgaggaccgc
     1201 ctgcatggcg cctgcgcgct gcaactcgag caggtcagcc acaccctcga gggcgccgcc
     1261 gaggtcgtcg accagccggc gccgccgcag agcccgatca ccctgccccc gggcacgacc
     1321 accagcctgc tcggcccgct cgacgatgac gcgagcacca cgagcccgga cgccgacccc
     1381 tccgacgagg accgcttcct cgccttcctc ggccccgcgc cgctgcgcag catgacgccg
     1441 accctcaacc cgccggagcg cagcgagggg cgcggctgga agtcggcgct cgagcacgcc
     1501 atccgcccgg ccctgcaccg cgcccgcggc accctgcgcg ccgccgcggc cagcttcacc
     1561 gatggcaccg cgctcgactc gctgcgcgcc gaggtcgagg cgacgctggt ccgccagccc
     1621 gagcagctga gcttcgacat tcaccccgag gactcgagcg tcgtcgccca ggacgtcccc
     1681 ccgacccgcg tcgaggtgcc gatgcgccgc tggctgctgg agaccctcaa gccgccgctg
     1741 ctggcgacga tctccgagtt tggcgcccgc atcgccgccg ccgtcaaggg cgccaccacc
     1801 gagctcgagc gcatcgagac cgtgctcgac tatcacctct tcatcgtcga ggacggccgc
     1861 gtcgccgagg agcgcgacga gtcggcgcgc gccggcctcg gccacggcgt caggctcgtg
     1921 caacagctcg ccgcgcgcct caaccacgag gtcgcgcgga cctatcgctg gttcatcgcc
     1981 gagagctcga tgcacatgga cgacgccctc gccccgctgc gagcccaccg tcccgaggag
     2041 atcacccaga cgctgaccgc cctcgcgcag cgccgcacca cgccgtggtg gaagcacacc
     2101 ggcctcggcc ggctgcgccg cggcgcgatc gacgtctacg gcaaggccgc cccgctggtc
     2161 cgcgaggtcg tccgcgacgt gcgcggcctc ctgcgcggcg accagccgcg cctcggctat
     2221 tgggacctgg cgatcgccag cgacctcgcc cccgacgacc gcctgccggc cctctatcgc
     2281 cgcctgttcg gcgagatccc gctcggcctc gccgacctct atcaaccacg acccgcgctc
     2341 gaggcgatct gtcgccgggt cttcgagaca tggctctcgg gacagccccg gcgcgccgag
     2401 cagcgcggcg ccctgctcgt caccggcgac cgcggcgcgg gcaagcgcac cctcgtcaac
     2461 cgcgtgcgcg ccgagctgca cggcgagctg ccggtccgct ggctcccgct cggcccgcac
     2521 gtcgccggcg aggcccgcct gagcgccgcg atcagcgagg ccaccggcgc gccgccggcg
     2581 agcaacttcg gcgagctcgc ccggaccctg cgcctgctcg accagcgcca ggcgatcgtg
     2641 ctcgagaacg ccgagcagct gttctttcgc acccccgagg gcctcgcgca ggtccagtcc
     2701 ttcctcgacc tcgtgcgcgc caccgacgac gcgatcctgt ggatcgtgct gatggtccag
     2761 c
//
LOCUS       DASWNJ010000705         3279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_683030, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000705 DASWNJ010000000
VERSION     DASWNJ010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683030"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1428)
                     /locus_tag="VGB85_11760"
     CDS             complement(<1..1428)
                     /locus_tag="VGB85_11760"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0134755.1"
                     /translation="MPREDRVEDAGQPFRIGPYTVLRRIGRGAMGVVYSAYDEQLDRK
                     IALKHLRGAPHEPGTLGQSRLLREAQALARVSHPNVVQVYEVGAHEREVYIAMEFVRG
                     RTLREWVRGEVRAWREILTVYIQAGRGLAAAHAAGLVHRDFKPDNVMLGDDGRVRVMD
                     FGLARALDEPGAALQAPSPHSSQDLSLSLTAAGRIVGTPAYMAPEQHIGGVTDARTDQ
                     FAFCVALYEGLYGQRPFAGDDLRALSASVTHGRLREPSRRLVPRWLRRIVLRGLAVDP
                     ARRWPSMQALLVEVERGQARARRELVAATLAGLGVLALGAWGWHEHTRAREAETCAAA
                     GASIDEVWHDAARERVRGAFAATGVGHAVTTAEKVLPWIDRQATAWADARAAVCMEAT
                     RDIGDPALRDRSTWCLDERRMAIESLVAELSRPDVAMVQRAVAVAAALEPVSQCRDPL
                     LLAHLTVPRRPTAPRCGPSRPSCCAP"
     gene            complement(1561..1797)
                     /locus_tag="VGB85_11765"
     CDS             complement(1561..1797)
                     /locus_tag="VGB85_11765"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0134756.1"
                     /translation="MKTPLPSITLDSLDLEAAERLLCVKALETSGSIVEAAKILGVTR
                     HALKRRIIKHKIDWPPPHSLRKGAQGPGASDTEG"
     gene            complement(2030..2182)
                     /locus_tag="VGB85_11770"
     CDS             complement(2030..2182)
                     /locus_tag="VGB85_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134757.1"
                     /translation="MAQGAPPRSRPVWLGAMNVGFHLYPVLLQRSQRARLRAIMRRMQ
                     TQGRGA"
     gene            2444..>3279
                     /locus_tag="VGB85_11775"
     CDS             2444..>3279
                     /locus_tag="VGB85_11775"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF036305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HEY0134758.1"
                     /translation="MSTAQAPQPPTDNQNLVKWVTEMAALTQPDRIVWCDGSEEEKAR
                     LTELAVSEGVLTELDQSKRPGCYYSRSNPNDVARVENLTFICTPTKEDAGPTNNWMDP
                     AEAYKKLGGLFEGAMRGRTMYVIPYVMGPLGSAMAKIGVELTDSVYVVLNMRIMTRMG
                     KGALDMLGSSNDFNRGLHCTLDINPERRFICHFPQDNTIWSVGSGYGGNVLLGKKCLA
                     LRIGSYLGKKEGWLAEHMLILGVESPEGEKHYVAAAFPSACGTTNFAMLIPPQHYKGW
                     KVT"
BASE COUNT          480 a         1211 c         1145 g          443 t
ORIGIN      
        1 cggcgcgcag cagctcggcc tggacggccc gcagcgcggc gcggtcggcc tccggggcac
       61 cgtcaggtgg gcgagcagca gcgggtcgcg gcattgtgac accggctcga gcgcggcggc
      121 gacggcgacg gcgcgctgga ccatcgcgac atcggggcgc gacagctcgg cgacgagcga
      181 ctcgatcgcc atgcgccgct cgtcgaggca ccacgtcgat cgatctcgca gcgccgggtc
      241 gccgatatcc ctcgtggcct ccatgcacac cgccgcgcgg gcgtccgccc acgccgtcgc
      301 ctggcggtcg atccacggca gcaccttctc ggcggtcgtc accgcatggc cgacgccggt
      361 cgccgcgaac gccccacgca cgcgctcgcg ggccgcgtcg tgccacacct cgtcgatgct
      421 ggccccggcg gcggcgcagg tctcggcctc gcgcgcgcgc gtgtgctcgt gccagcccca
      481 cgcgccgagc gcgagcacgc cgaggccggc cagcgtcgcg gcgaccagct cgcgccgggc
      541 tcgcgcctgt ccgcgctcga cctcgaccag cagcgcctgc atgctcggcc agcggcgcgc
      601 cgggtcgacc gcgagcccgc gcagcacgat cctccgcagc cagcgcggca ccagccggcg
      661 cgagggctcg cgcaggcggc cgtgggtgac gctggcgctc agcgcacgca ggtcgtcgcc
      721 ggcgaacggg cgctggccgt acaggccctc gtacagcgcg acgcagaacg cgaactggtc
      781 ggtgcgcgcg tcggtcaccc cgccgatgtg ctgctcgggc gccatgtatg ccggcgtgcc
      841 gacgatccgc cccgcggccg tcagcgacag cgacaggtcc tgcgagctgt gcggcgacgg
      901 ggcctgcagc gccgcgccgg gctcgtcgag cgcgcgcgcg aggccgaagt ccatcacgcg
      961 cacgcggccg tcgtcgccga gcatcacgtt gtccggcttg aagtcacgat gcacgagccc
     1021 ggccgcgtgg gcggccgcga gcccgcgccc ggcctggatg tacacggtca ggatctcgcg
     1081 ccacgcccgg acctccccgc ggacccactc gcgcagggtc cggccgcgga cgaactccat
     1141 tgcgatgtac acctcgcgct cgtgcgcccc gacctcgtag acctgcacga cgttgggatg
     1201 cgacacgcgg gcgagcgcct gggcctcgcg cagcagcctc gactgcccga gcgtgccggg
     1261 ctcgtgcggg gcgccccgca ggtgcttgag cgcgatcttg cgatccagct gctcgtcgta
     1321 ggcgctgtag accacgccca tcgccccgcg accgatgcgc cgcagaaccg tgtacggccc
     1381 gatgcgaaac ggctgcccgg cgtcctctac cctgtcctcc cgcggcaccg tcatccctcg
     1441 accgcgccac tataggcgcc gggagaaaca cggttcaagc cgtctgcatg gcgacgcggc
     1501 ccggacggcc gatgctcgtg ggcgcgcaca tcgacgcaaa ccgccgctgc gccggtctgc
     1561 tcagccttcc gtgtccgacg cgccggggcc ttgcgccccc ttgcgcagcg aatgcggggg
     1621 cggccagtcg atcttgtgct tgatgatccg gcgcttgagg gcgtgccggg tgacgcccag
     1681 gatcttggcc gcctcgacga tgctgcccga ggtctcgagc gccttgacgc aaagcaagcg
     1741 ctctgcggcc tccaggtcga gggagtcgag ggtgatgctg ggcagcggcg tcttcatgga
     1801 tgcatgtatg cccggccacc ggcttggctt caaaaacgcg aatgtgtggc ttctcgctca
     1861 agatgcgcgc gagtacgccc ttgcgcgcat tgctttttcg cggcctcgcg agggtccagg
     1921 ggcgcgccgg cgccggtgcc cgccccgcgc ccgatgcggg cacttgtgcc cacttcgatc
     1981 gccgcggcgg atgaaacggc tcagggccga catcacgctc gaatcgccgt cacgcgccgc
     2041 ggccctgcgt ctgcatccgc cgcatgatcg cccgcaggcg cgcgcgctgg ctgcgttgca
     2101 gcagcaccgg atacaggtga aagccgacgt tcatcgcgcc gagccacacc ggacgcgagc
     2161 gaggcggtgc gccctgggcc atcgggacgc accctggcac gtcgtggtcc acgacccacg
     2221 gatcgccgca cgggctggtc cggcgcggct caggaagtga gatcacgccc gtctgtgcac
     2281 agcgggcccg cgaccgtgcg ccgcgggtct gcggcccggg aagtcccttg cgagcgccgg
     2341 ccgcagcgaa cggcgttgcg cggccgggtt cgtgcgctgg gtcgcgtgca tacggcctgg
     2401 cgcccgcgcc tgcgcaggtc cggcgaagct aaggttgccg cgcatgtcga ccgcccaagc
     2461 tccccagccc ccgaccgaca accagaacct cgtgaagtgg gtcaccgaga tggccgcgct
     2521 gacgcagccc gatcgcatcg tgtggtgtga cggcagcgag gaggagaagg cgcggctgac
     2581 cgagctcgcg gtcagcgagg gcgtcctgac cgagctcgac cagagcaagc ggcccggctg
     2641 ctactactcg cgcagcaacc ccaacgacgt cgcgcgggtc gagaacctga ccttcatctg
     2701 cacgccgacc aaggaggacg ccggtccgac caacaactgg atggacccgg cggaggccta
     2761 caagaagctc ggcgggctgt tcgagggcgc gatgcgcggc cgcacgatgt acgtgatccc
     2821 gtacgtgatg ggcccgctcg gctcggcgat ggcgaagatc ggcgtcgagc tgaccgacag
     2881 cgtgtacgtc gtgctcaaca tgcgcatcat gacgcgcatg gggaagggcg ccctcgacat
     2941 gctgggcagc tccaacgact tcaaccgcgg cctgcactgc acgctcgaca tcaaccccga
     3001 gcgccgcttc atctgccatt tcccgcagga caacacgatc tggtcggtcg gctcgggcta
     3061 cggcggcaac gtgttgctcg gcaagaagtg cctggccctg cgcatcggaa gctacctcgg
     3121 caagaaggaa ggctggctcg ccgagcacat gctcatcctc ggcgtcgaga gcccggaggg
     3181 cgagaagcac tacgtcgcgg cggcgttccc cagcgcgtgc ggcacgacca acttcgccat
     3241 gctcatcccg ccccagcact acaagggctg gaaggtcac
//
LOCUS       DASWNJ010000706         3923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_648630, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000706 DASWNJ010000000
VERSION     DASWNJ010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648630"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..2389)
                     /locus_tag="VGB85_11780"
     CDS             complement(161..2389)
                     /locus_tag="VGB85_11780"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEY0134759.1"
                     /translation="MRRHLLAAALLTSLACAPGPAPAVAPVPGGATPDAHLQARLDLA
                     GEFLRLPELVQDSLVAPRWLRDGDRLVFWSAQTTGGGTWMLAEARTGAIRPLLPAEAL
                     REQVARALGRTVEPLQFYDFAVAADQRSIVFEVEGTALALGLEDARITALAADDPAAR
                     SLSPLHFVSPDGRAVAGQQADGFVVRAAGGASIERAGEAQHGWRIPDDPWSPDGRFLV
                     VWREDLRDVHQLPIVDTGAVLERVTWVPYAKVGTPLPRPQLHVVEPATGRVTPIAGIE
                     GETHDWFVGWLPDGREALCLHLSRDGKRLDLTAVDPRSGKRRLVLREERRDTFVAGLD
                     LAVGGAARQVKPLPDGKGLLWLSERDGWRHVHHYDLDGRHIRQVTRGAFPVHEVVAVD
                     PDGDTLYVLASAEPGAPYEQLLYRGSLRGGELTRMSAASGMHRIGFAPSGDYYVDAWS
                     SRTQPRLRELVATRGDARVRLTTADPAALVKLGHRPPEALTVLAADGVTPLHGALYKP
                     RNFDPGKRYPVIAYIYAGPFTTVVPWSFLGSGESIEAAALAQMGFVVLLLDPRGGPGR
                     SKAFQDAHYGRIGQTEIPDHVAALRQAAADRPWMDLERVGIHGHSWGGYFALRGMLTA
                     PELFKAGYAGAPGALEEEALINEPYLGLPASNPAGYKAGSNEALAARLRGHLKLMHGT
                     ADVNAPVSTTMRMAAALIAAGKRFELLIMPGEPHSPGPPAYRYYRDDVHTFFARTLGG
                     PR"
     gene            2503..>3923
                     /locus_tag="VGB85_11785"
     CDS             2503..>3923
                     /locus_tag="VGB85_11785"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HEY0134760.1"
                     /translation="MKSRLAPICIVAAFCLTACSDDTSLIDAPPAGDSSTTTTAAPET
                     GTSTPTTGDAGPTTTTGPGLTTTGPDETSTGKEDDSSSGPPPDPTTGAPEECPDRPSG
                     FYKDCVNGAVCDGAVDDATCLVDVPDAESFGICSLLCQDDCDCFADPGSSGAVSACVP
                     LLEGGEKACVLDCSGGKTCPSGSYCYSDLDICVYGVKVDKEADLDLDAVSLDDHTLAP
                     GEMTALNFEVDNLGTVTVDAGFIVRAVLSKNDIFGDDDDIIVIEGSFPEPVEPSTPTG
                     WYIDIEIPADVFDGVYHVAMGLDTEDDVAESDETNNVAFDPVLLIISGNPMPGNADLG
                     LENALAAAHQVLQGAQTSFGFTVKNLAAADVPAYSVGLYYSKDPQVTTADTLICTHTD
                     ADGLAGMAQEAKQVSCAVPKLVGAHYFGAIVDPADMLDELDEANNADVDADPVTITAP
                     VVDLVMGAVTSDDNTVDTGQLVTL"
BASE COUNT          585 a         1411 c         1398 g          529 t
ORIGIN      
        1 gtgttcggct tcgacgaccg cggtcgcatc gcgccggggc tgcgcgccga cctggtgctc
       61 gtgcacggcg atcccggcgt cgacatcaag gcgacgcgcg cgatcgagag ggtgtggaag
      121 ctcggcgtcg aggtcgagcg cccgctgccc tgagcccgct ttagcgcggg ccgccgagcg
      181 tgcgcgcgaa gaatgtgtgc acgtcgtcgc gataatagcg gtatgcgggc gggccggggc
      241 tgtgcggctc gcccggcatg atcagcagct cgaagcgctt gccggcggcg atcagcgccg
      301 cggccatgcg catcgtcgtc gacacgggcg cgttgacgtc ggcggtgccg tgcatcagct
      361 tgaggtggcc gcgcaggcgg gccgcgagcg cctcgttgga tcccgctttg taacccgcgg
      421 ggttgctcgc cggcaggccg aggtagggct cgttgatcag cgcctcctcc tcgagcgcgc
      481 ccggggcacc ggcgtagccg gccttgaaca gctccggcgc ggtcagcatg ccgcgcagcg
      541 cgaagtagcc gccccacgaa tggccgtgga tgccgacgcg ctcgaggtcc atccacgggc
      601 ggtcggcggc cgcctggcgg agcgcggcga cgtgatcggg gatctccgtc tggccgatcc
      661 gcccgtaatg cgcgtcctgg aacgccttgc tgcggcccgg tccgccgcgc gggtcgagca
      721 gcagcacgac aaagcccatc tgcgccagcg cggcggcctc gatcgactcg ccgctgccga
      781 ggaagctcca tggcacgacg gtggtgaacg gccccgcgta gatatacgcg atcaccgggt
      841 agcgcttgcc gggatcgaag ttgcgcggct tgtagagcgc gccgtgcagc ggcgtcacgc
      901 cgtcggcggc gagcacggtc agcgcctccg gcgggcgatg gccgagcttc accagcgcgg
      961 ccgggtcggc ggtggtcagg cgcacgcggg catcgccgcg ggtggcgacc agctcgcgca
     1021 gccgcggctg cgtgcgtgac gaccaggcgt cgacataata atcgcccgac ggcgcgaagc
     1081 cgatccggtg catgcccgac gccgcggaca tgcgcgtcag ctcgccgccg cgcaggctcc
     1141 cgcgatagag cagctgctcg tacggggcgc ccggctcggc ggacgcgagc acgtacaacg
     1201 tgtcgccgtc ggggtcgacc gcgacgacct cgtgcaccgg gaaggcgccg cgcgtgacct
     1261 gacgaatgtg ccggccgtcg aggtcatagt gatggacgtg tcgccagccg tcgcgctcgg
     1321 acagccacag cagccccttg ccgtccggta atggtttgac ctgccgcgcc gcgccgccga
     1381 ccgcgaggtc gaggccggcg acgaaggtgt cgcgccgctc ctctcgcagc acgagccggc
     1441 gcttgccgga ccgcggatcg accgcggtga ggtcgaggcg cttgccgtcg cgcgagaggt
     1501 gcaggcacag cgcctcgcgg ccgtccggga gccagccgac gaaccagtcg tgggtctcgc
     1561 cctcgatgcc ggcgatcggc gtcacgcggc cagtggcggg ctcgaccacg tggagctgcg
     1621 ggcggggcag gggcgtgccc accttggcat agggcaccca ggtcacgcgc tcgagcaccg
     1681 cgccggtgtc gacgatcggc agctgatgaa cgtcgcgcag gtcctcgcgc cagacgacga
     1741 ggaagcggcc gtcgggcgac catggatcgt cggggatccg ccagccgtgc tgcgcctcgc
     1801 cggcgcgctc gatgctcgcc ccgccggccg cgcgcacgac aaagccgtcc gcctgctgcc
     1861 cggcgaccgc gcggccgtcc gggctcacga agtgaagcgg ggacagcgag cgcgcggccg
     1921 ggtcgtccgc cgcgagcgcg gtgatccggg cgtcctccaa tccgagcgcg agcgcggtgc
     1981 cctcgacctc gaagacgatg ctgcgctgat cggccgcgac ggcgaagtca taaaattgca
     2041 gcggctcgac cgtccggccg agcgcgcgcg cgacctgctc gcgcagggcc tccgcgggca
     2101 gcagcggacg gatcgcgccg gtgcgggcct ccgcgagcat ccatgtgccg ccgcccgttg
     2161 tctgtgccga ccagaacacc aggcggtcgc cgtctcgcag ccaccgcggc gcgaccaggc
     2221 tgtcctgcac gagctccggc agccgcagga attcgccggc gaggtcgagg cgcgcttgca
     2281 agtgcgcgtc cggggtcgcg ccgccgggca ccggcgcgac cgcgggcgcc gggccgggcg
     2341 cgcaggcgag gctcgtcaac agggcggcgg cgaggaggtg gcggcgcatg tccggagggt
     2401 agggagccgg gccgtgtatg accagcatat ctttcgatcg caaacaggcg gccgaggtgc
     2461 cgccacattg acctcccgtc cgcgatccgg caccctcgtg tggtgaaatc tcggctcgct
     2521 cctatctgta ttgtcgcggc cttttgcctg accgcctgct cggacgacac ctcgctcatc
     2581 gacgccccgc ctgccggtga cagctcgacg accacgacgg ccgcgccgga gacgggcacg
     2641 tcgacgccga cgacgggtga cgccggcccg acgaccacga cggggcccgg cctgaccacc
     2701 acggggcccg acgagacctc gaccggcaag gaggacgaca gctcgagcgg accgccgccg
     2761 gacccgacga ccggcgcgcc cgaggaatgc cccgaccgac cgtccgggtt ttacaaggac
     2821 tgcgtcaacg gggcggtgtg cgacggcgcc gtcgacgacg cgacctgcct cgtcgacgtc
     2881 ccggacgccg agagcttcgg gatctgctcg ctgttgtgcc aggacgactg cgactgcttc
     2941 gccgacccgg gcagctccgg cgcggtctcg gcctgcgtgc ctctgctcga aggcggggag
     3001 aaggcctgcg tgctcgactg ctcgggcggc aagacatgcc cgtccgggag ctactgctac
     3061 tccgacctcg acatctgcgt gtacggcgtc aaggtcgaca aggaggcgga cctcgatctc
     3121 gacgccgttt ctctcgacga ccacacgctc gcgcccgggg agatgacggc cctcaacttc
     3181 gaggtcgaca acctcgggac ggtcaccgtc gacgccggtt tcatcgtccg cgccgtgctc
     3241 tcgaagaacg acatcttcgg cgacgacgac gacatcatcg tgatcgaggg gtccttcccc
     3301 gagcctgtcg agccctcgac gccgaccgga tggtatatcg acatcgagat ccccgcggac
     3361 gtcttcgacg gcgtgtacca cgtcgccatg gggctcgaca ccgaagacga cgtcgccgag
     3421 tcggacgaga ccaacaacgt cgcgttcgac cccgtcctgc tgatcatctc cggcaacccg
     3481 atgcccggca acgccgacct cggcctcgag aatgcgctcg cggcggcgca ccaggtgctt
     3541 cagggcgccc agacgagctt tggcttcacc gtcaagaacc tcgccgccgc ggacgtgccg
     3601 gcgtacagcg tcggcctgta ctattcgaag gacccgcagg tcacgaccgc cgacaccctg
     3661 atctgcaccc acaccgacgc cgatggcctc gccggcatgg cccaggaggc gaagcaggtc
     3721 agctgcgccg tgccgaagct cgtcggcgcc cactacttcg gcgcgatcgt cgacccggcg
     3781 gacatgctcg acgagctcga cgaggccaac aacgccgacg tcgacgccga cccggtcacg
     3841 atcaccgccc cggtcgtcga cctcgtgatg ggggccgtca ccagcgacga caacaccgtc
     3901 gacaccggcc agctcgtcac gct
//
LOCUS       DASWNJ010000707         4050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_154177, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000707 DASWNJ010000000
VERSION     DASWNJ010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154177"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VGB85_11790"
     CDS             complement(<1..274)
                     /locus_tag="VGB85_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134761.1"
                     /translation="MRPGVLRVALVAASLAAPRAAASAAPALNLEWSAPAACPRGDEV
                     REMASALLGTPVTARGSASTQVRARVRASAGAFVLTIETRSSSGTDR"
     gene            complement(335..757)
                     /locus_tag="VGB85_11795"
     CDS             complement(335..757)
                     /locus_tag="VGB85_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134762.1"
                     /translation="MRAVIGAGLVGLLGCGRAPAGTAAPVEPAPASEPEGDRGAVPRV
                     HVEPVTVGPKWSPAMVKRVVRTRMPEIERCQAGALDAPPVTLRFAIGADGLVSSAEAL
                     VAGGADSSHPAHACVLAEIRALRFPADMTGLIITYPLR"
     gene            complement(761..2446)
                     /locus_tag="VGB85_11800"
     CDS             complement(761..2446)
                     /locus_tag="VGB85_11800"
                     /inference="COORDINATES: protein motif:HMM:NF036933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HEY0134763.1"
                     /translation="MATKFGGGATLVMLAALTGCGDSGREDSQGTGPVTVGTLTTLTG
                     QTAGTGSSGEPTGTSDPGVTESGTSQTATSEAMTSGLPKFDLGTPPGIDQGEVEGPII
                     PTNCTEAASGKSTVGCKFYAVDMDAHDQVETSQYAVGVANVQLDGMATVTIEAKENGV
                     WQTVGGPQAIAPLQLFTFNLADRHQDDTGVRVGGAYRVTSDVPVVAYQFNPVDGASSF
                     LSDAAMLYPAAALDTVNHVTAWTSMMDNSNTFQHSYATVIATVDGTMVTVDPKTTTVG
                     GAGVPAGGPGAPFMVPLQEGDVLNIAVDTLGTSMTGTKINSNADHPIAVFAGQECALI
                     PESVCCCDHMEEQLAGVRLWGKNFIAARMPVRNPGSPEQTLWQIYAAENGTNVTVNYD
                     PGVTGIPSQNFTLNQGEMNQMYVSGPPGEEGDFEVIADKPIAVYGYMVGAQNFGSDVG
                     DPAMVAMAPSEQFLPRYVILVPGTWVNDVAVVTRPTGAQILLDGVAISDAMFNPVAAS
                     GFEVARVPVPDGVHVFDGGDEPFSVIIVGYDQYDSYAYLGGTGTGKINPNPPG"
     gene            2590..3870
                     /locus_tag="VGB85_11805"
     CDS             2590..3870
                     /locus_tag="VGB85_11805"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0134764.1"
                     /translation="MTRLSLTGLAHLACLTSLSCLAPLALGACGDDDAGGSVSAASTG
                     NETGGSTTNTNATPGTAAASTGDEPTAGSASGSASATTTGDTTPTTGVTTEEPTTTPQ
                     TSTTGDPTTATTSTDTTDTAVTASSESGDTTTGEMAAGTVRFVAFGDTGEGNEAQYAV
                     GQAMAALCADKGCDFGLLLGDNFYDSGVDGVDDPQWQEKFEMPYEDIDFPIYATLGNH
                     DYGGNGIGVDFDGKKAQYQIDYTKMSQIWKLPAKYHSFKLEHAEFWDLDTNQIMTDPI
                     NGDAGPQQAWLANGLAASKATWKIAFGHHPYISNGDHGNAGDYENLEGIPLPFVTGDS
                     VKEFMEDTVCGKVDVYLCGHDHNLQWLAPKCGTEFIVSGAGSKAEGLPGSNPAAFGLP
                     ETEGFLWVEIKDNTFTGVFYDQTGKELFTKTIMK"
     gene            complement(3871..>4050)
                     /locus_tag="VGB85_11810"
     CDS             complement(3871..>4050)
                     /locus_tag="VGB85_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134765.1"
                     /translation="STCAGWTSAELTKAARIGLNAPPPGDAAALAEWKAGKQWLSLKN
                     RPCQTANRLYCIEAT"
BASE COUNT          636 a         1534 c         1289 g          591 t
ORIGIN      
        1 gtcggtcggt gccgctgctc gatcgggtct cgatcgtcag cacgaaggcc ccggcgctcg
       61 cgcgcacgcg cgcgcgcacc tgtgtgctcg cggagccgcg ggcggtcacc ggtgtgccga
      121 gcagcgcgct cgccatctcg cggacctcgt cgccgcgcgg gcaagcggcg ggcgccgacc
      181 actcgaggtt gagcgccggc gctgcgctgg cggcggcccg cggcgccgcc agcgaggcgg
      241 ccacgagcgc gacgcgtagg accccgggac gcacggcccg cagggtatca ggcttgcgcc
      301 ccgaacccgc accgactcgg cgcccacgcg cggatcatcg cagcggatac gtgatgatca
      361 gcccggtcat gtccgcggga aagcgcagcg cccggatctc ggcgagcacg cacgcgtgcg
      421 ccgggtggct gctgtcggcc ccgcccgcca cgagcgcctc ggcgctgctc accagcccgt
      481 ccgcgccgat cgcaaagcgc agcgtcaccg gcggcgcgtc gagggctccc gcctggcagc
      541 gctcgatctc cggcatacgc gtgcgaacga cccgcttgac catcgcgggt gaccacttcg
      601 ggccgaccgt caccggctcg acatgcaccc gcggcaccgc cccgcgatcg ccctccggct
      661 cgctcgcagg ggccggttcg accggcgcgg ccgtacccgc gggtgcccgc ccgcagccga
      721 gcagccccac gagtcccgcg ccgatcaccg cacgcatggc tcagccgggc gggttcgggt
      781 tgatcttccc ggtgccggtg ccgccgaggt acgcgtagct gtcgtactgg tcgtagccga
      841 cgatgatcac gctgaagggc tcgtcgccgc cgtcgaacac gtgcacgccg tcgggcacgg
      901 gcacgcgggc gacctcgaag ccgctcgccg cgaccgggtt gaacatcgcg tcggagatcg
      961 ccaccccgtc gagcaggatc tgtgcgccgg tcgggcgcgt gaccaccgcg acgtcgttga
     1021 cccacgtgcc cggcaccagg atcacgtagc gcggcaggaa ctgctcgctc ggggccatcg
     1081 ccaccatcgc cgggtcgccg acgtcgctgc cgaagttctg cgcgccgacc atgtatccgt
     1141 acaccgcgat cggcttgtcg gcgatgacct cgaagtcacc ctcctccccg ggcggcccgg
     1201 acacgtacat ctggttcatc tcgccctgat tgagcgtgaa gttctgcgac gggatcccgg
     1261 tgacacccgg gtcgtagttg accgtcacgt tggtgccgtt ctccgccgcg tagatctgcc
     1321 acagcgtctg ctccgggctg ccggggttgc gcaccggcat gcgggccgcg atgaagttct
     1381 tgccccacag gcgcacgccc gcgagctgct cctccatgtg gtcgcagcag cacaccgact
     1441 cggggatcag cgcgcactcc tggccggcga acaccgcgat cggatgatcg gcgttgctgt
     1501 tgatcttcgt gcccgtcatc gacgtcccga gcgtgtcgac cgcgatgttg agcacgtcgc
     1561 cctcctgcag cggcaccatg aacggcgccc ccgggccgcc cgccggcacg cccgcgccgc
     1621 cgaccgtcgt cgtcttcggg tcgaccgtca ccatcgtccc gtccaccgtc gcgatcaccg
     1681 tcgcgtagct gtgctggaag gtgttgctgt tgtccatcat cgacgtccac gccgtcacgt
     1741 gattgaccgt gtcgagcgcc gccgccgggt acagcatcgc cgcgtcggac aggaacgagc
     1801 tcgcgccgtc gaccgggttg aactgatacg cgaccaccgg cacgtccgag gtcacgcgat
     1861 aggccccgcc gacgcgcacg cccgtgtcgt cctggtggcg gtcggcgagg ttgaaggtga
     1921 acagctgcag cggcgcgatc gcctgcggtc cgccgaccgt ctgccacacg ccgttctcct
     1981 tggcctcgat cgtcaccgtc gccatcccgt cgagctgcac gttcgccacc ccgaccgcat
     2041 actgcgaggt ctcgacctgg tcgtgcgcgt ccatgtcgac cgcgtagaac ttgcagccga
     2101 ccgtgctctt gcccgacgcc gcctcggtgc agttcgtcgg gatgatcggc ccctccacct
     2161 cgccctgatc gatgccgggc ggggtcccga ggtcgaactt cggcaggccc gaggtcatcg
     2221 cctccgaggt cgccgtctgg ctcgtaccgc tctccgtcac ccccgggtcg ctcgtccccg
     2281 tcggctcgcc cgagctgccc gtgcccgccg tctgcccggt cagcgtcgtc agcgtgccca
     2341 ccgtcaccgg cccggtgccc tgcgagtcct cgcgccccga atctccgcat cccgtgagcg
     2401 ccgccagcat caccagcgtc gcacccccac cgaacttcgt ggccatcatc cccgcaccat
     2461 acggaaaacc gccgcgcccg aaaagctggg gtcaccatgc aggcaccgcg gagagcagcc
     2521 cccggatcct ccgcgagccg cgagttctcg cacgactgcc gacacggggc ccaggtatac
     2581 aatcgcgcca tgacgcgcct ctcgcttacc ggtctcgctc acctcgcctg cctcaccagc
     2641 ctgtcctgtc tcgccccgct cgcgctcggc gcgtgtggcg acgacgacgc gggaggcagt
     2701 gtcagcgccg cgagcaccgg caacgagacc ggcggctcga ccaccaacac caacgcgacg
     2761 ccgggcaccg ccgccgcgag cacgggggac gagccgaccg ccggcagcgc gagcggcagc
     2821 gccagcgcga cgacgactgg cgataccacg ccgaccaccg gcgtcaccac cgaggagccg
     2881 accacgactc cgcagacctc gaccacggga gatccgacga ccgcgaccac gagcaccgac
     2941 accaccgaca ccgccgtcac tgccagctcg gagagcggcg acaccacgac cggcgagatg
     3001 gctgcgggca ccgtccgctt cgtcgctttc ggcgacaccg gcgagggcaa cgaggcccag
     3061 tacgcggtcg gtcaggcgat ggcggcgctg tgcgccgaca agggctgcga cttcggcctg
     3121 ctgctcggcg acaacttcta tgatagcggc gtcgacggcg tcgacgaccc gcaatggcag
     3181 gagaagttcg agatgcccta cgaggacatc gacttcccga tctacgccac gctcggcaat
     3241 cacgactatg gtggcaacgg catcggcgtc gacttcgacg gcaagaaggc gcaataccag
     3301 atcgactaca ccaagatgag ccagatctgg aagctgccgg ccaagtatca cagcttcaag
     3361 ctcgagcacg ccgagttctg ggacctcgac accaaccaga tcatgaccga cccgatcaac
     3421 ggcgacgccg gcccgcagca ggcgtggctc gccaacggcc tcgccgccag caaggcgacc
     3481 tggaagatcg cgttcggtca ccacccctac atctccaacg gcgaccacgg caacgccggc
     3541 gactacgaga acctcgaggg catccccctg cccttcgtca ccggcgactc cgtcaaggag
     3601 ttcatggagg ataccgtctg cggcaaggtc gacgtgtacc tctgcggcca cgaccacaac
     3661 ctccagtggc tcgcacccaa gtgcggcacc gagttcatcg tctccggcgc cggctccaag
     3721 gccgagggcc tccccggcag caacccggcc gccttcggct tacccgagac cgagggcttc
     3781 ctgtgggtcg agatcaaaga caacaccttt acgggtgtct tctatgatca gaccggcaag
     3841 gagctgttca cgaagacgat tatgaaatga tcaggtcgcc tcgatgcagt agaggcgatt
     3901 cgcggtttgg cagggcctgt tttttaaact gagccactgc ttcccggcct tccactcggc
     3961 gagcgccgcg gcgtcccctg gcggcggcgc gttcaacccg atacgcgccg ccttggtcag
     4021 ctcggccgag gtccagccgg cacacgtcga
//
LOCUS       DASWNJ010000708         1825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_578391, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000708 DASWNJ010000000
VERSION     DASWNJ010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578391"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VGB85_11815"
     CDS             <1..793
                     /locus_tag="VGB85_11815"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEY0134766.1"
                     /translation="NYLWFDSYHARLLPRHERGQTTGLLPANTFVRTVEPDSPAARAG
                     IAPGDRVLAVGDRPVTRWETVAVLLDRQGEQSLELTVQSPGQEPRAVTVTQQIRSYRG
                     IYKQERSYLWFGAEPYHSRDIPAAEPIRGRFTYAVRSAVVETGAMLKMMWTSLRQMVT
                     LERGVEELSSVVGIFNVAGTAAEQGPGQFLLLMALLSINLGFVNLLPIPILDGGHLMF
                     FTLEALRRRPLSQRAREIASAIGLIIILLLLLIALRNDVMRLWMD"
     gene            795..1523
                     /gene="tsaB"
                     /locus_tag="VGB85_11820"
     CDS             795..1523
                     /gene="tsaB"
                     /locus_tag="VGB85_11820"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEY0134767.1"
                     /translation="MATIWLLALDTSTPCTALALGRVDRDTGAHELVAHATHDDRKAP
                     ASSLLAPRISALLGEAGISARQLGAVGCGVGPGMFTGTRVALATAKGLAFALPCPLFV
                     VSTLAAVAGGAARDGEVLALLDARRGEVYAGRFTLGPAGPQMQGEARCCPIAQVLLDM
                     AGGPDLQVIGPGAAAQAEALVEYTTLPTIGATGAGLWRAAARGVLQEAPADLDNVDAV
                     YLRASYAELGTNTPRRTPYRSPFA"
     gene            complement(1628..>1825)
                     /locus_tag="VGB85_11825"
     CDS             complement(1628..>1825)
                     /locus_tag="VGB85_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134768.1"
                     /translation="LDVRPGLYTRFQGRPSLPNTTGGAVFDIDEVASGKLGFAAAVSG
                     AIKSGVCTRYPLSPFGSEPPQ"
BASE COUNT          238 a          636 c          697 g          254 t
ORIGIN      
        1 caactatctc tggttcgact cgtaccacgc gcgcctgctg ccgcgacacg agcgcgggca
       61 gacgaccggg ctgctgccgg ccaacacctt cgtgcgcacg gtggagccgg actcgccggc
      121 ggcgcgcgcc gggatcgcgc cgggggaccg cgtgctcgcg gtcggcgacc ggccggtgac
      181 gcgctgggag accgtcgcgg tgctgctgga tcgtcagggc gagcagtcgc tcgagctgac
      241 ggtgcagtcg cccgggcagg agccgcgggc ggtcacggtg acccagcaga tccgcagcta
      301 tcgcgggatc tacaagcagg agcggagcta cctgtggttc ggcgccgagc cgtatcacag
      361 ccgcgacatc ccggcggccg agccgatccg cgggcgcttc acctacgcgg tgcgatcggc
      421 ggtcgtcgag accggcgcga tgctcaagat gatgtggacc agcctgcgcc agatggtcac
      481 gctcgagcgc ggcgtcgagg agctgagctc ggtggtcggt atcttcaacg tcgccggcac
      541 cgcggccgag cagggtcccg ggcagttcct gctgctgatg gcgctgctga gcatcaacct
      601 cggcttcgtc aacctgctgc cgatcccgat cctcgacggc ggacacctga tgttcttcac
      661 gctcgaggcg ctacgacgcc ggccgctgag ccagcgcgcg cgggagatcg ccagcgcgat
      721 cggcctgatc atcatcctgt tgctgctcct catcgccctg cgcaacgacg tcatgcgcct
      781 gtggatggac tgacatggcg acgatctggc tgctcgcgct cgacacctcg acgccgtgca
      841 cggcgctggc gctcgggcgc gtcgaccggg acaccggcgc gcacgagctc gtcgcacacg
      901 cgacccacga cgaccgcaag gcgccggcgt cgagcctgct cgcgccgcgg atctcggcct
      961 tgctcggtga ggcggggatc tcggcgcggc agctcggcgc ggtcggctgt ggcgtcggac
     1021 cgggcatgtt caccggcacg cgcgtggcgc tggcgaccgc gaaggggctg gccttcgccc
     1081 tgccctgccc gctgttcgtc gtgtcgacgc tggcggccgt cgccggcggg gccgcgcgtg
     1141 acggcgaggt gctggcgctg ctcgatgcgc ggcgcggcga ggtctacgcg ggccgcttca
     1201 cgctcgggcc cgccgggccg cagatgcagg gcgaggcgcg ctgctgtccg atcgcgcagg
     1261 tccttttgga catggccggg ggaccagact tgcaggtgat cggcccgggc gcggcggccc
     1321 aggcggaggc gctggtcgag tacacgacgc tgccgacgat cggcgcgacc ggtgcggggc
     1381 tgtggcgcgc cgcggcccgc ggtgtgctgc aagaggcgcc cgcggacctc gacaacgtcg
     1441 acgcggtgta cctgcgggcc agctacgcgg agctcggcac taacacgccg cgccggaccc
     1501 cgtatcgctc gcccttcgcg tgagcgcggc ggcggcgcac gagcaacgac aggggcaccg
     1561 gggacgcgag cggcacgagg cgcagtgcgc caccgtggcg tgagatgctc gcccggggcg
     1621 cccgggctca ctgcggcggt tcgctgccga agggcgagag cggatagcgg gtgcacacgc
     1681 cgctcttgat cgccccgctg accgcggccg cgaagccgag cttgccgctg gcgacctcgt
     1741 cgatgtcgaa cacggccccg ccggtggtgt tgggcagcga gggccggccc tggaagcggg
     1801 tgtacaggcc ggggcgcacg tcgag
//
LOCUS       DASWNJ010000709         4269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_610896, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000709 DASWNJ010000000
VERSION     DASWNJ010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610896"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VGB85_11830"
     CDS             complement(<1..507)
                     /locus_tag="VGB85_11830"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY0134769.1"
                     /translation="MQLVDIGVNLGHRSFAGDRDAVLERAVKAGVRTMICTGTSVRGS
                     QDALALARSRPGTLYATAGVHPHDAARCDAGTLDALRALADRPEVVAIGECGLDFNRD
                     FSPRPVQIEWFARQVVLAKELGLPLFLHERDAAEAFLDVLDAEQPAPETVVVHCFTGD
                     GPTLKQYLR"
     gene            complement(843..2414)
                     /locus_tag="VGB85_11835"
     CDS             complement(843..2414)
                     /locus_tag="VGB85_11835"
                     /inference="COORDINATES: protein motif:HMM:NF017541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TROVE domain-containing protein"
                     /protein_id="HEY0134770.1"
                     /translation="MANLDLFSSLIKRASKQSKTAPADAVNAAGGAAYQRSDKQALAQ
                     YVTTGCLNGTFYASAQEQLAEVTRLSAQVEPAFLARAAVYAREQGFMKDMPALLLAVL
                     ASRDGALFEAVFHRICDNGKMVRNFVQILRSGATGRKSLGTRPKRAVQRWLKNRNDAQ
                     LLAASVGASPSLADIIKMVHPQPDTPSRRALYGWLLGRKHEASDLPALVREFEDFKAG
                     RTKQPPDVPFQMLTAQALTKHQWRAIADNGSWQMTRMNLNTFMRHEVFDDAALTRRIA
                     HRLGSAEQVKRARAFPYQLLMAYKQADSNLPRLVREALQDAMEAAIANVPAIDGKVYL
                     LPDVSGSMHSPVTGHRDGATTKVRCIDVAALVAAAFLRRNRDAEVLPFHDKVLPRSLS
                     ASDGVLKNAEILARLPSGGTDCSVPLRHLNERKAKGELVIFISDNESWIDRARKGRGT
                     GVLHEWDQFRKRNPRAKLVCIDVQPSATTQAPDRPDILNVGGFSDAVFELVAAFASGG
                     LGPDHWVGVIDAVTI"
     gene            2634..3260
                     /locus_tag="VGB85_11840"
     CDS             2634..3260
                     /locus_tag="VGB85_11840"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEY0134771.1"
                     /translation="MQTTALTRDFPGGRRLCDEPLIHVLDDLLTPAECAHIVEVAEPK
                     MRRSQVSGATGGKISQGRTNALTWVRHDTDAIVLAAATRIAAAVGLPLSHAESLQVIH
                     YEPGQEYRTHFDAYDLSTEKGQRYTERGGQRLVTALAYLGAVEGGTTDFPRLGISIAP
                     RPGSVLVFHNCHPGTNVCDRRTEHQGRPPLRGDKWAFNLWFHERPYWP"
     gene            complement(3261..>4269)
                     /locus_tag="VGB85_11845"
     CDS             complement(3261..>4269)
                     /locus_tag="VGB85_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134772.1"
                     /translation="ELLGTCEPAGLCSFPDAMCPSGKKYGELAGDQSGQCVGDGGGST
                     SSGTSSPTSTTSVSVTGSGTFDPTTDPGSGTTFDPTTGLTGASDTTLTTDPSTTSITT
                     DPVTSDPGTTTGPTCGQVGEGCIDGTCCGACAVCQDNVCQAGGLDVAASVCGSVCSAC
                     TAEGQCGSQPGGTTCKSNCSDIVWQQVGEVTKTTCYAYADLAVDSVCDAGGHCIPPAL
                     ENCPDPSMVPGSEVPLASCDNACLQGADLCTQGQPASTITPGSYCALNVETAGCKSAC
                     TDGNLSVVPASCNSEGVCAYADMSSCAPYLCNPDNNSCFAKCSDSTQCATGKCQGSKC
                     M"
BASE COUNT          652 a         1441 c         1503 g          673 t
ORIGIN      
        1 gcgcaggtac tgcttcagcg tcgggccgtc gccggtgaag cagtgcacga cgaccgtctc
       61 cggcgccggc tgctccgcgt cgagcacgtc caaaaatgcc tcggcggcgt cacgctcgtg
      121 caggaacagc ggcaggccga gctccttcgc cagcacgacc tggcgggcga accactcgat
      181 ctgcacgggc cgcggcgaga agtcgcggtt gaagtcgagg ccacactcgc cgatcgccac
      241 cacctcgggg cggtccgcga gcgcgcgcag ggcgtcgagc gtgccggcgt cgcagcgggc
      301 cgcgtcgtgc gggtgcacgc cggcggtcgc gtacagcgtc cccggtcggc tgcgcgccag
      361 cgcgagcgcg tcctggctgc cgcgcacgct ggtgccggtg cagatcatcg tgcgcacacc
      421 ggccttgacg gcgcgctcga ggaccgcgtc ccggtcgccc gcgaacgagc gatgaccgag
      481 gttgactccg atgtcgacga gctgcatggc gcccgcagtc tatgcgaaaa aaggcgaccg
      541 cgacgagggt gggcgaaacg ttcgtgctct accgttgagc tatcgccccg cacgagaagt
      601 ggagcgaccc ggagtcgaac cgagatcccg tcatccaaaa tgtagtttcg cccgcattcg
      661 cggtcaaagt cgtctcaggg ccggggccca ggagggaaat aggtgccacg acgagaattt
      721 cacgtgacgg tttcagatgt agtcacgcga gcattcgtgg cgggccgcga cgagatccgg
      781 gggacgtggg atcggaaaag atgtagtccc gtcgggcatt cgcggcccaa gctcattcgc
      841 cgtcagatcg tgacggcgtc gatgacaccg acccagtggt cggggccgag gccgccgctt
      901 gcgaaggcgg cgaccagctc gaagacggcg tcggagaagc cgccgacgtt gaggatgtcc
      961 gggcggtccg gcgcctgcgt ggtggcggac ggctggacat cgatgcacac cagcttggcc
     1021 cgcgggttgc gcttgcgaaa ctggtcccac tcgtgcagca caccggtacc ccggcccttg
     1081 cgcgcgcggt cgatccacga ctcgttgtcc gagatgaaga tcaccagctc gcccttcgcc
     1141 ttgcgctcgt tgaggtggcg cagcggcacc gagcagtccg tgccgcccga gggcagcctg
     1201 gcgaggatct ccgcgttctt gagcacgccg tcggacgcgc tcaggctgcg cgggagcacc
     1261 ttgtcgtgga agggcaggac ctccgcgtcg cggttgcgcc gcaaaaacgc ggctgccacg
     1321 agcgcggcga catcgatgca acggaccttc gtggtcgcgc cgtcgcggtg gcccgtcacg
     1381 ggcgagtgca tcgagcccga gacatcgggc agcaggtaga ccttgccgtc gatcgccggc
     1441 acgttggcga tcgccgcctc catggcgtcc tgcagcgcct cgcggacgag ccgaggcagg
     1501 ttcgagtccg cttgcttgta ggccatcagc agctggtagg ggaaggcgcg ggcgcgcttg
     1561 acctgctcgg ccgagccgag acgatgcgcg atccggcggg tcagcgccgc gtcgtcgaag
     1621 acctcgtgac gcatgaaagt gttgaggttc atgcgcgtca tctgccacga gccgttgtcg
     1681 gcgatcgcac gccactggtg cttcgtcagc gcctgggcgg tcaacatctg gaacggcacg
     1741 tcgggcggct gcttggtgcg gccggccttg aagtcctcga attcgcggac gagcgccggc
     1801 aggtcgctcg cctcgtgctt gcgaccgagc agccagccgt acagcgcccg ccgcgacggc
     1861 gtgtcgggct gcgggtgcac catcttgatg atgtcggcca gcgagggcga ggcgccgacg
     1921 ctcgcggcga gcagctgcgc gtcgttgcgg ttcttgagcc agcgctgcac cgcccgcttg
     1981 ggccgcgtgc cgagcgactt gcggcccgtc gcgccgcttc gcaagatctg cacgaagttg
     2041 cggaccattt tgccgttgtc gcagatgcga tgaaacaccg cctcgaacag cgcgccgtcg
     2101 cgggacgcga gcaccgcgag caacagcgcc ggcatgtcct tcatgaagcc ctgctcgcgg
     2161 gcgtataccg ccgcgcgggc gaggaacgct ggctcgacct gcgcgctcag ccgcgtgacc
     2221 tccgcgagct gctcctgcgc cgaggcgtag aaggtgccgt tgaggcagcc ggtcgtcacg
     2281 tactgcgcga gcgcctgctt gtcgctgcgc tggtacgccg cgccgccggc cgcattgacg
     2341 gcgtcggccg gcgcggtctt gctctgcttc gacgcgcgct tgatcaggga ggagaagagg
     2401 tcgaggttgg ccatgacggc gctccggtgc cggcccgcga ccgtcgcggc gccgttacgg
     2461 agctacatga gcacgccgcg gaccacgccg ccttgacgcg cttgatatca atgcttacgc
     2521 ctacttatcg actccgacga ccggtgccgg ccgcaaaagt acgcgtccgc cgtatcggga
     2581 tttatccgaa tttctaaagg gccgcagagt cggaaccgcg tatcctgggc ctcatgcaga
     2641 cgacggcgct gacgcgcgac ttcccgggcg gccggcggct gtgcgacgag ccgctgatcc
     2701 acgtcctcga cgatctgctg acgccggcag agtgtgcaca tatcgtcgag gtcgcggaac
     2761 cgaagatgcg gcgctcgcag gtcagcgggg cgaccggcgg aaagatcagt cagggccgca
     2821 ccaacgcgct gacgtgggtc cgccacgaca ccgatgccat cgtgctcgcc gcggcgacgc
     2881 ggatcgccgc ggccgtcggg ctgccgctgt cccacgccga atctctacag gtcatccact
     2941 acgagcccgg ccaggagtat cgcacccact tcgacgcgta cgacctctcg accgagaagg
     3001 gccagcgtta caccgagcgc ggcgggcagc gcctggtgac cgcgctcgcg tacctcggcg
     3061 cggtcgaggg cggcacgacc gacttcccgc gcctcggcat ctcgatcgcg ccgcgccccg
     3121 gcagcgtgct cgtgtttcac aactgccacc cgggcaccaa cgtctgcgac cggcgcaccg
     3181 agcatcaggg ccggccaccg ctgcgcggcg acaagtgggc gtttaatttg tggttccacg
     3241 agcggccgta ctggccgtga ctacatgcac ttcgaaccct ggcacttgcc cgtcgcgcac
     3301 tgggtgctgt cggagcactt cgcgaagcat gaattgttgt cggggttaca gagatacggg
     3361 gcgcacgagg acatgtccgc gtaggcgcag acgccctcgc tgttgcagct cgcgggcacg
     3421 acgctgaggt tgccgtcggt gcaggccgac ttgcaccctg cggtctcgac gttgagcgcg
     3481 caatacgagc caggggtgat cgtgctcgcc ggctggccct gcgtgcacag atcggcgccc
     3541 tgcaggcacg cgttgtcgca gctggcgagg gggacctcgc tgcccgggac catcgagggg
     3601 tcggggcaat tctcgagggc cggcgggatg cagtggccgc ccgcgtcaca cacgctgtcg
     3661 accgcgagat cggcgtaggc gtaacaggtg gtcttggtga cttcgcccac ctgctgccac
     3721 acgatgtcgc tgcagttcga cttgcaggtg gttccacccg gctgcgaccc gcactgacct
     3781 tccgcggtgc aggccgagca caccgagccg cagacggacg cggcgacgtc gaggccgccg
     3841 gcctgacaga cgttgtcctg acacaccgcg cacgccccgc agcaggtccc gtcgatgcag
     3901 ccctcgccga cctggccgca ggtcggtccg gtcgtcgtgc ccgggtcgct ggtcaccggg
     3961 tcggtggtga tcgacgtcgt cgacgggtcg gtcgtcagcg tcgtgtcgga ggcgccggtt
     4021 agcccggtcg tcggatcgaa ggtcgtgccg gagccggggt cggtcgtcgg atcgaaggtg
     4081 ccgctgccgg tgaccgacac cgatgtcgtc gaggtcggcg agctcgtccc gctgctcgtg
     4141 ctcccgccgc cgtcaccgac gcactggccg gactgatctc cggcgagctc gccatacttc
     4201 ttgccggacg ggcacatcgc atcggggaag ctgcacaggc cggcgggctc gcaggttccc
     4261 agcaattcg
//
LOCUS       DASWNJ010000710         3679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_506409, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000710 DASWNJ010000000
VERSION     DASWNJ010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506409"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /gene="modA"
                     /locus_tag="VGB85_11850"
     CDS             <1..549
                     /gene="modA"
                     /locus_tag="VGB85_11850"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY0134773.1"
                     /translation="VGQIAEGAPVDVFAAADEPQMQRAIATGRITRAEVFARNRLVLI
                     SPVDGAVAGPADLGRPGLRLVLAGPGVPAGAYARQALQELGVAEAALANLVSNEENVR
                     GVVGKIVAGEADAGVVYASDVTPAVADKLRVLPLTVTVQPRYEVAVLADSPEASLAAA
                     FVAELSGPGGQTLLVHHGFLLP"
     gene            546..1358
                     /locus_tag="VGB85_11855"
     CDS             546..1358
                     /locus_tag="VGB85_11855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0134774.1"
                     /translation="MTDPSDMSLRPGRPGQAHAVLAALGGAALVFFALPLIGLVSRAT
                     PAGLWRQLGSSTVQAALWLSLEVSLVSLALCLVFGAPLAFLLARVEFPGRSLARGLVS
                     LPMVLPPVVGGIALLSAFGRRGLLGEALGTIGVFLPFTTAGAVVAVTFVSMPFLVMTL
                     EAALASVDPRLEQAAATLGASRWRILRTVTLPAIRPALAAGLALCWARGLGEFGATIT
                     FAGNLEGRTQTMPLAVYDALQTDPEAAITLGLVLLAVSLTLLMALRGRISRR"
     gene            1355..2071
                     /locus_tag="VGB85_11860"
     CDS             1355..2071
                     /locus_tag="VGB85_11860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0134775.1"
                     /translation="MSGLEIDAEVVRGNFKMALRCALPEGCTAALLGPNGSGKSTMVE
                     LLAGLVPASRGSVTLAGRVLERAPGLRMPPQARGLGVVFQGLALFPALTVIDNVAYGL
                     RSRGVGQAQARAQAQAWLERFEVGALADRRAGELSGGQAQRVALARAMIVRPPLLLLD
                     EPLSALDVEARDAARSRLRGWLAEVPGVKLLVTHDLEDARAVADRLLVLDRGRLVAQG
                     PVEELLAAPPPFLAAMMRRA"
     gene            complement(2083..2877)
                     /locus_tag="VGB85_11865"
     CDS             complement(2083..2877)
                     /locus_tag="VGB85_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134776.1"
                     /translation="MRRLSSTISLLPLWWALLPACSQTNTDHCGNREGHPTCYQQDKA
                     TPFCSICVATNNGCVDKRPDDGCFATTAPATSTAADSSSSTVDPTTGGSESNTSTTGS
                     TSTGPTTDPTTGTTTTTGQTDTSTTTPGTTTTGDTSTTSTDTSTSTGDTVDTVDTLGT
                     STSTTGDTDTTAGTTMEPDPCGNNMIDEGEVCDGTNNNKETCKSVYPDKWGGGMLACN
                     EGCQSFNDAKCCIGLGQKCGGLNPDGACCPGLTCKVDMLLGTVCKN"
     gene            complement(2905..>3679)
                     /locus_tag="VGB85_11870"
     CDS             complement(2905..>3679)
                     /locus_tag="VGB85_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134777.1"
                     /translation="ASSQPAPAEADEVGASDGASNDDVAVIARAGLEIDTTAAGPSGP
                     VMLSRLEEVGNLELRRAEILPRRAGDDPVIRVKVEVRGQEGDTYAIFSEVTVRGQVVE
                     GSAREVVCSLCTEGEAVDRARSELLRLVPFVRARFRAAAKPADNKPPPVVTSAAEDKK
                     KPLGTYGKAGIGLLAGGAVLLGTGIGLSVREPRPDPDDPLREINTRPVGYAVLGVGAA
                     AIVTGAVLLVLDRRKARRVAHLAPMIGRGSAGLLLVGRF"
BASE COUNT          405 a         1130 c         1542 g          602 t
ORIGIN      
        1 gtcgggcaga tcgccgaggg ggcgccggtc gacgtgttcg cggcggccga cgagccgcag
       61 atgcaacgag cgatcgccac cggacgtatt acccgtgcgg aggtgttcgc gcgtaaccgg
      121 ctggtgctga tctcgccggt cgatggcgcg gtcgcagggc ccgcggacct cgggcggccc
      181 gggctgcggc tggtgctcgc ggggccaggc gtgccggccg gggcgtacgc gcggcaagcc
      241 ctgcaagagc tcggggtcgc cgaggcggcg ctcgccaacc tggtgtccaa cgaggagaac
      301 gtgcgcgggg tggtcggaaa gatcgtcgcg ggagaggccg acgcgggggt ggtatacgcg
      361 agcgacgtga cgccggcggt cgccgataaa ttgcgcgtgc tgccgctgac ggtgacggtg
      421 cagccgcgct acgaggtcgc ggtgctggcc gacagcccgg aggcgtcgct cgcggcggcg
      481 ttcgtggccg agctgtccgg gccgggcggg cagaccttgc tggtgcacca cgggttcctg
      541 ctgccgtgac tgacccttcg gacatgtcgc tacggccagg tcgtccgggc caggcgcacg
      601 cggtgctggc ggcgctcggc ggggcggcgc tggtgttctt cgcgctgccg ctgatcgggc
      661 tggtcagcag agcgacgcca gcggggctgt ggcggcagct ggggtcgtcg acggtgcagg
      721 cggcgctgtg gctgtcgctc gaggtgtcgc tggtgtcgct ggcgctgtgc ctggtgttcg
      781 gggcgccgct ggcgttcctg ctggcgcggg tcgagttccc ggggcggtcg ctggcgcgcg
      841 ggctggtcag cctgccgatg gtgctgccgc cggtggtcgg ggggatcgcg ctgctgtcgg
      901 cgttcgggcg gcgcgggctg ctcggggagg cgctgggcac gatcggcgtg ttcctgccgt
      961 tcacgacggc gggcgcggtg gtggcggtga ccttcgtgtc gatgccattt ttggtgatga
     1021 cgctcgaggc ggcgctggcg agcgtcgacc cgcggctcga gcaggccgcg gcgacgctgg
     1081 gggcgagccg ctggcggatc ctccggacag tgacgctgcc ggcgatccgg ccggcgctgg
     1141 cggccgggct ggcgctgtgc tgggcgcgtg ggctcggtga attcggggcg acgatcacgt
     1201 tcgcgggcaa cctggagggg cgcacgcaga cgatgccgct ggcggtctac gacgcgctgc
     1261 agaccgaccc ggaggcggcg atcaccctcg ggctggtgct gctggcggtg tcgctgacgc
     1321 tgctgatggc gctgcgtgga aggatctccc ggcgatgagc gggctcgaga tcgacgcgga
     1381 ggtcgtgcgc gggaatttta agatggcgct gcgctgtgcg ttgccggagg ggtgcacggc
     1441 ggcgctgctg gggcccaatg ggtcgggcaa gtcgacgatg gtcgagctgc tcgcagggct
     1501 ggtgccggcg agtcgcggca gcgtgacgct ggcaggacgg gtgctcgagc gggcgccagg
     1561 gctgcgaatg ccgccgcagg cgcgtgggct cggggtggtg tttcaggggc tggcgctgtt
     1621 ccccgcgctg acggtgatcg acaacgtggc gtacgggctg cggtcgcgtg gggtcgggca
     1681 ggcgcaggcg cgagcgcagg cgcaggcgtg gctcgagcgc ttcgaggtcg gtgcgctggc
     1741 ggatcggcgc gcgggggagc tgtcgggcgg gcaggcgcag cgggtcgcgc tggcgcgagc
     1801 gatgatcgtg cggccgccgt tgctgctgct cgacgagccg ctgtcggcgc tggacgtcga
     1861 ggcccgcgac gcggcacgct cacgactgcg tggctggctg gccgaggtgc cgggggtcaa
     1921 attgctggtg acgcatgacc tcgaggacgc gcgggcggtc gcggatcggc tgctggtgct
     1981 cgaccgcggg cggctggtcg cgcaggggcc ggtcgaggag ctgctggccg cgccgccgcc
     2041 gttcctggcg gcgatgatgc ggcgagcttg aggcgggggc tgtcagttct tgcagacggt
     2101 gccgagcagc atgtcgacct tgcaggtgag accgggacag caggcgccgt cggggttgag
     2161 gccgccgcac ttctggccga gtccgatgca gcacttggcg tcgttgaagc tctggcaacc
     2221 ttcgttgcaa gcgagcatac cgccgcccca cttgtcgggg tacacgctct tgcaggtctc
     2281 tttgttgttg ttggtgccgt cgcagacctc tccctcgtcg atcatgttgt tgccgcacgg
     2341 atcgggctcc atcgtggtgc cggcggtcgt gtcggtgtca cccgtcgtgc tcgtgctggt
     2401 gccgagggtg tcgaccgtat cgacggtgtc accggtgctc gtgctcgtgt cggtggacgt
     2461 cgtgctcgtg tcgccggtgg tcgtcgtgcc cggggtggtc gtgctggtgt cggtctggcc
     2521 ggtcgtggtc gtggtgccgg tggtcgggtc cgtggtcggg ccggtggagg tcgagccagt
     2581 ggtcgaggtg ttggactcgg agccgccggt ggtcgggtcg acggtgctcg agctcgagtc
     2641 ggccgcggtc gaggtggcgg gggcggtggt ggcgaagcag ccgtcgtcgg ggcgcttgtc
     2701 gacgcagccg ttgttggtcg cgacgcagat gctgcaaaag ggcgtcgcct tgtcctgctg
     2761 atagcaggtc gggtggccct cacggtttcc gcagtgatcg gtgttggtct ggctgcaggc
     2821 cgggagcagg gcccaccaca gcgggagcag ggagatcgtc gaggacaggc ggcgcatggg
     2881 tccgggctcc gggaacagtg ttgcctagaa gcgtcccacg agcagcaggc cggcggatcc
     2941 tcgaccgatc atcggagcga ggtgggccac acggcgtgcc ttgcgccgat cgaggaccag
     3001 caagaccgcg cccgtgacga tcgcggccgc gccgacgccg agcacggcgt agccgacagg
     3061 ccgcgtgttg atctcgcgca gcgggtcgtc cgggtcgggg cgtggctcgc ggaccgagag
     3121 gccgatgccg gtgccgagca gcaccgcgcc gccggcgagc aggccgatgc cggccttgcc
     3181 gtaggtgccg agcggcttct tcttgtcctc ggcggcggac gtgacgaccg gcggcggctt
     3241 gttgtcggcc ggcttcgcgg cggcccggaa gcgggcgcgt acgaacggca cgaggcgcag
     3301 cagctcgctg cgcgcgcggt cgacggcctc accctcggtg cacagcgagc agacgacctc
     3361 gcgcgcgctg ccctcgacga cctggccgcg cacggtgacc tcgctgaaga tcgcgtaggt
     3421 gtcgccctcc tggccgcgga cctcgacctt gacgcggatg acggggtcgt ccccggcgcg
     3481 acgcgggagg atctccgcgc ggcgcagctc gaggttgccg acctcctcga ggcgcgacag
     3541 catcaccgga ccggaggggc ccgcggcggt cgtgtcgatc tcgaggcctg cgcgggcgat
     3601 gaccgcgacg tcgtcgttgc tcgcgccgtc gctggcaccg acctcgtcgg cctcggccgg
     3661 ggccggctgc gacgaggcg
//
LOCUS       DASWNJ010000711         2402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_326023, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000711 DASWNJ010000000
VERSION     DASWNJ010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326023"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2402)
                     /locus_tag="VGB85_11875"
     CDS             complement(<1..>2402)
                     /locus_tag="VGB85_11875"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0134778.1"
                     /translation="GLAHAHGRRDDFGAPIGIVHRDISPHNIMLSLAGTVKILDFGIA
                     RTAARARLQAEAQPSFAVARGDGTEVTIQGKIAYMSPEQAVGGPLDQRSDIYSLGVVL
                     YELLSGELVFRHRDRLVALEQVRTRPLAPLRSIAPEIPEELAAVVDRALARDPEARWD
                     TARALQSALAGFLHRADPVVDDEVLSAFVARYAGPAPVAPPALDDATREIEGSDPSQG
                     GPVARPQLQRVVVLKAALVPALPGEKPAAPTRFLALARDIAYKRDAQVRQLDATGVSL
                     VFGAVLDTGDTDETAVRVALALREAIGEAAPGYGIGVALAGLALPLLRGPGGLEQAEV
                     PHEQARRLQAIASAAIDGPVMLTGDLAERLQRGWRIGPARGLDLGSEPDLSREPADAD
                     PLERASPLLGPVREVDERPLAPGRVVLVGRELEQKALRDSFAEAIRARSSRAVLLVGS
                     AGVGKRALIERFVASLPRGACVILRGAGQWRRRNVPLGVFLEVLREFFEVDRTTTAAD
                     IEARLISEGVAGAGPLAHSLVVALGLGLHAPALPGTEIDPLERRDRLWQLMRRLVRAL
                     AQRRPVLIVLENLHFVDEHSHNLLQAWMHRPHGLPLLGLCTVRPGPRAESTRALPGVH
                     TIELRELDEQARRELIVRRFEDPEEAEPIAAAILARTGGNPLFIEETLADLLRRGILG
                     WNASGRQLQVRQRGATIELHPDIEAALHARIDALDPRDRALVDAAAVLGQSFRVDELA
                     ALAQQSTSEVEHSLGRLAEHGMFTAMTTGARFTTARFATVSLHEVVKLQLGAAKTEAL
                     HR"
BASE COUNT          297 a          862 c          960 g          283 t
ORIGIN      
        1 gcggtgcagc gcctcggtct tggccgcgcc gagctggagc ttgacgacct cgtgcaggct
       61 gacggtcgcg aagcgggcgg tcgtgaagcg ggcgccggtg gtcatcgcgg tgaacatgcc
      121 gtgctcggcg agccggccga gcgagtgctc gacctccgag gtgctctgct gcgcgagcgc
      181 ggcgagctcg tcgacgcgaa agctctggcc gagcaccgcg gcggcgtcga cgagggcccg
      241 gtcgcgcggg tcgagggcgt cgatgcgggc gtgcagcgcg gcctcgatgt cggggtgcag
      301 ctcgatcgtc gcgccgcgct ggcggacctg cagctggcgc ccgctggcgt tccacccgag
      361 gatcccgcgg cgcagcaggt cggcgagggt ctcctcgatg aacagcgggt tgccgccggt
      421 gcgcgcgagg atcgccgcgg cgatcggctc ggcctcctcg gggtcctcga agcggcgcac
      481 gatcagctcg cggcgggcct gctcgtcgag ctcgcgcagc tcgatcgtgt gcacgccggg
      541 cagggcgcgc gtcgactcgg cgcgcggacc gggacgcacg gtgcacagac cgagcagcgg
      601 cagcccgtgc gggcgatgca tccacgcctg cagcaggttg tggctgtgct cgtcgacgaa
      661 gtgcaggttc tcgagcacga tcagcaccgg ccggcgctgg gccagcgcgc gcaccagccg
      721 gcgcatcagc tgccacaggc gatcgcggcg ctcgagcggg tcgatctcgg tgcccggcag
      781 cgcgggcgcg tgcaggccga gcccgagcgc gacgacgagg ctgtgcgcca gcgggcccgc
      841 gcccgcgacc ccctcgctga tcaggcgcgc ctcgatgtcg gcggcggtgg tcgtgcggtc
      901 gacctcgaag aactcgcgca gcacctcgag gaacacgccg agcggcacgt tgcgccggcg
      961 ccactgaccc gcgccgcgca ggatgacgca cgctccgcgg gggaggctcg cgacgaaccg
     1021 ctcgatcaac gcccgcttgc cgaccccggc cgagccgacg agcagcacgg cccgcgagct
     1081 gcgcgcgcgg atcgcctcgg cgaacgagtc gcgcagcgcc ttttgctcga gctcgcggcc
     1141 gacgagcacg acgcggccgg gggcgagcgg gcgctcgtcg acctcgcgca ccgggccgag
     1201 cagcggcgag gcgcgctcga gcgggtcggc gtcggctggc tcgcgggaca ggtcgggctc
     1261 ggagccgagg tcgagcccgc gcgcgggccc gatccgccag ccgcgttgca ggcgctcggc
     1321 gaggtcgccg gtcagcatca ccgggccgtc gatcgccgcg ctggcgatcg cttgcaggcg
     1381 ccgcgcctgc tcgtgcggga cctcggcctg ctcgagcccg ccgggcccgc gcagcagcgg
     1441 caacgcgagg cccgcgaggg cgacgccgat cccgtagccg ggggccgcct cgccgatagc
     1501 ctcgcgcagc gcgagcgcga cgcgcacggc ggtctcgtcg gtgtcgccgg tgtcgagcac
     1561 cgcgccgaac accagcgaca cgccggtcgc gtcgagctgg cggacctggg cgtcgcgctt
     1621 gtaggcgatg tcgcgggcga gcgcgaggaa gcgggtcggc gcggccggct tctcgcccgg
     1681 cagcgcgggc accagcgccg ccttgagcac gacgacgcgc tgcagctggg gccgcgcgac
     1741 cggcccgccc tgcgaggggt cgctgccctc gatctcgcgg gtcgcgtcgt cgagcgcggg
     1801 cggggcgacc ggcgccggcc cggcgtagcg cgcgacgaag gcgctgagca cctcgtcgtc
     1861 gacgacgggg tcggcgcggt gcaggaagcc ggcgagcgcc gactgcaagg cgcgcgcggt
     1921 gtcccagcgc gcctcggggt cgcgggcgag cgcgcggtcg accaccgcgg cgagctcctc
     1981 ggggatctcc ggggcgatcg atcgcagcgg cgccagcggc cgcgtgcgca cctgctcgag
     2041 cgcgacgagc cggtcgcggt ggcgaaacac cagctcgccc gagagcagct cgtagagcac
     2101 gacgccgagc gagtagatgt cgctgcgctg atcgagcggc ccgccgacgg cctgctcggg
     2161 cgacatgtac gcgatcttgc cctgaatggt aacttcggtg ccgtcgccgc gggcgaccgc
     2221 gaagctcggc tgggcctcgg cctgcaggcg agcgcgggcc gccgtgcgcg cgatgccgaa
     2281 gtcgaggatc ttcaccgtgc ccgcgagcga cagcatgatg ttgtgcgggc tgatgtcgcg
     2341 gtgcacgatg ccgatcggcg cgccgaagtc gtcgcggcgg ccgtgcgcgt gggccagccc
     2401 gg
//
LOCUS       DASWNJ010000712         1679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_189795, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000712 DASWNJ010000000
VERSION     DASWNJ010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189795"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1679
                     /gene="rpoB"
                     /locus_tag="VGB85_11880"
     CDS             <1..>1679
                     /gene="rpoB"
                     /locus_tag="VGB85_11880"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014525059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEY0134779.1"
                     /translation="DTYSCKVIPSRGAWLEFEVDKRDAVGVRIDRKRKQPVTVLLKAL
                     GWDDAKILERYGDYASMRGTLEKDSTTGQDDALIDIYRKLRPGEPPTRESAQTLLENL
                     FFNAKRYDLAKVGRYKVNKKLGLELPVDQGVLTEDDILATIDYIVKLHAGEAGFEVDD
                     IDHFGNRRLRTVGELIQNQIRLGLARMERVVRERMTTQDVEAITPQTLINIRPVVASI
                     KEFFGTSQLSQFMDQTNPLSGLTHKRRLSALGPGGLSRERAGFEVRDVHPSHYGRMCP
                     IETPEGPNIGLIGSLATFGRVNAFGFVETPYRKVVNGEVTDQVDYLTADEEDKHVIAQ
                     ANALDSSGSFAEKRVLVRKKGGEVDYIAPAGVDYIDVSPRQICSVATAMIPFLEHDDA
                     NRALMGSNMMRQAVPLLRSEAPLVGTGMEHRAAVDAGDVVVAEQAGVVEDLSADYVTV
                     MHDDGTRRTYRLSKFRRSNQGTCINQKPIVAEGQRVEAGQVIADGPCTDDAEMALGKN
                     LLVAFMPWEGHNYEDAIILSQRLVQDDVLTSIHIDEHEVDARDTKLGAEEIT"
BASE COUNT          304 a          609 c          543 g          223 t
ORIGIN      
        1 gacacctact cgtgcaaggt gatcccgtcg cgcggcgcct ggctcgagtt cgaggtcgac
       61 aagcgcgacg ccgtcggcgt gcgcatcgac cgcaagcgca agcagcctgt cacggtgctg
      121 ctcaaggcgc tcggctggga cgacgcgaag atcctcgagc gctacggcga ctacgcctcc
      181 atgcgcggca ccctggagaa ggacagcacc accggccagg acgacgcgct gatcgacatc
      241 taccgcaagc tgcgcccggg cgagccgccg acgcgcgagt cggcccagac gctgctcgag
      301 aacctgttct tcaacgccaa gcgctacgac ctcgccaagg tcggccgcta caaggtgaac
      361 aagaagctcg ggctcgagct cccggtcgac cagggcgtcc tcaccgagga cgacatcctc
      421 gcgacgatcg actacatcgt gaagctgcac gccggcgagg cgggcttcga ggtcgacgac
      481 atcgaccact tcggcaaccg gcgcctgcgc acggtcggcg agctcatcca gaaccagatc
      541 cgcctgggcc tggcccgcat ggagcgcgtc gtccgcgagc gcatgacgac ccaggacgtc
      601 gaggcgatca cgccgcagac cctgatcaac atccgccccg tcgtggcgag catcaaggag
      661 ttcttcggga cctcccagct cagccagttc atggaccaga ccaacccgct gtcgggcctg
      721 acccacaagc gccgcctgtc ggcgctgggc ccgggtggtc tgtcccgcga gcgcgccggc
      781 ttcgaggtcc gcgacgtgca cccgagccac tacggccgca tgtgcccgat cgagacccct
      841 gagggcccga acatcggcct gatcggctcc ctggcgacct tcgggcgcgt gaacgccttc
      901 ggcttcgtcg agacccccta ccgcaaggtg gtcaacggag aggtgaccga ccaggtcgac
      961 tacctcaccg ccgacgagga ggacaagcac gtcatcgccc aggcgaacgc gctcgactcc
     1021 tccggctcgt tcgccgagaa gcgcgtgctc gtccgcaaga agggcggcga ggtcgactac
     1081 atcgcgccgg ccggcgtcga ctacatcgac gtcagcccgc ggcagatctg ctcggtcgcg
     1141 accgcgatga tcccgttcct cgagcacgac gacgccaacc gcgcgctcat ggggtccaac
     1201 atgatgcgcc aggccgtgcc gctgctgcgc agcgaggcgc cgctggtcgg caccggcatg
     1261 gagcaccgcg cggctgtcga cgccggcgac gtcgtcgtcg ccgagcaggc gggtgtggtc
     1321 gaggacctct cggccgacta cgtcacggtg atgcacgacg acggcacgcg gcgcacgtac
     1381 cgcctgtcga agttccgtcg cagcaaccag ggcacctgca tcaaccagaa gcccatcgtg
     1441 gccgagggcc agcgcgtcga ggccggccag gtcatcgccg acggcccctg caccgacgac
     1501 gccgagatgg cgctcggcaa gaacctgctg gtcgccttca tgccgtggga gggccacaac
     1561 tacgaggacg cgatcatcct gtcgcagcgc ctcgtgcagg acgacgtgct cacctcgatc
     1621 cacatcgacg agcacgaggt cgacgcccgc gacaccaagc tcggtgccga ggagatcac
//
LOCUS       DASWNJ010000713         2766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_82668, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000713 DASWNJ010000000
VERSION     DASWNJ010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82668"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="VGB85_11885"
     CDS             complement(<1..913)
                     /locus_tag="VGB85_11885"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0134780.1"
                     /translation="MSAFVSASRDRESSTQMERTRVETASDTGSEQVSGDSMPSAPRK
                     ALLGRYSLLRKLGEGGMGVVYSAYDEELDRRVAIKLLRVSSGGDTEAASRMLREAQLM
                     AKLSHPNVVQVYDVGVVSRRVFLAMEFVQGVTLREWLHGTPTAPRPVPRPWPEVLAIY
                     IQAGQGLAAAHAAGLVHRDFKPDNVLVGTDGRARVLDFGLARSDAHADDLGVSTERND
                     DSLESTRSRQSRRSSEQPLTVAGTMIGTPAYMSPEQHLRVATDPRSDQFSFCVSLYEG
                     LYGERPFAGHSANELRGAVLTGRMRDPP"
     gene            1229..2293
                     /locus_tag="VGB85_11890"
     CDS             1229..2293
                     /locus_tag="VGB85_11890"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0134781.1"
                     /translation="MPVSGGATDVSESSGEPGTGPGTTGAPDTGEDSTSEATSGVSTT
                     GDTSTGGDSESGMSTGVGEGGCADGTQGCPCYGNMTCNDGLACEGDVCVPMQAAVCGD
                     GMLGGAEQCDAGVENGDTKLCKSDCTAQKCGDGFVGPGEACDDGNAVDDDACSNACVP
                     AACGDQVVQVGEACDDGNADPNDGCITCKAAACGDTFVQAGVEDCDDGNVNDGDGCSA
                     ACKKEAPKCGGNFTTDWCPQAGTKEQFTRCESVSNGGKTCNNPFVKYGSIDNGVPASH
                     DSNDFNAWCTQLGFAGFSGQVSYGSRPCDAPQGKLFGCTGWDENVWHWCDWQDGYWYS
                     QILDWHSCNDGQEITSITCQ"
     gene            2453..2668
                     /locus_tag="VGB85_11895"
                     /pseudo
     CDS             2453..2668
                     /locus_tag="VGB85_11895"
                     /inference="COORDINATES: protein motif:HMM:NF025602.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
BASE COUNT          411 a          867 c         1113 g          375 t
ORIGIN      
        1 gcggcgggtc gcgcatgcgg ccggtcagca cggcgccgcg cagctcgttg gcgctgtggc
       61 cggcgaacgg gcgctcgccg tagaggccct cgtagagcga gacgcagaag ctgaactgat
      121 cggagcgcgg gtcggtggcg acgcgcaggt gctgctccgg ggacatgtac gcgggggtgc
      181 cgatcatcgt gccggcgacg gtcagcggct gctcgctcga ccgccggctc tggcggctgc
      241 gcgtcgactc gagcgagtcg tcgttgcgct cggtcgagac gccgaggtcg tcggcgtggg
      301 cgtcgctgcg cgcgaggccg aagtcgagga cgcgggcgcg gccgtcggtg ccgacgagca
      361 cgttgtcggg cttgaagtcg cggtgcacga ggccggcggc gtgggcggcc gcgaggccct
      421 gtccggcctg gatgtagatc gccaggacct cgggccacgg gcgcggcacc gggcgcgggg
      481 ccgtcggggt gccgtgtagc cactcgcgca gggtgacgcc ctgcacgaac tccatcgcca
      541 agaacacgcg gcgcgagacg acgccgacgt cgtagacctg gacgacgttg ggatgcgaga
      601 gcttggccat cagctgggcc tcgcgcagca tgcgggacgc ggcctcggtg tcgccgccgg
      661 agctgacgcg cagcagcttg atcgcgacgc ggcggtcgag ctcctcgtcg tatgcggagt
      721 agacgacgcc catgccgccc tcgccgagct tgcgcagcag ggagtagcgg ccgagcagcg
      781 ccttgcgcgg ggcggacggc atgctgtcgc cggacacctg ttcgctgccg gtgtcgctgg
      841 ccgtctcgac gcgcgtgcgc tccatctgcg tcgaggactc gcggtcacgc gacgcggaca
      901 cgaacgcgga cacggacgat cgttcgtcgg gcccggcccg tccgctcgtg tccgaatcct
      961 cgccgcgcgc tgtcacgatc gccagcgtgg cagatgcccg tgggcccgac aagcgcgagg
     1021 gcgggcgctg tcgcgcggtg cgaatcgatt actgtctctc gggacaggtc gttcttgctc
     1081 gcgttgaccg gccggcgctt tgcttctacc gtgccattgc gtgctgctcg aacgacgatc
     1141 cctctccatc ctcgtctgcg catttgcgct cgccgcttgc gcgagcggtg aagagacgtc
     1201 gacaggcttc tcctcgctgc cgaacacgat gccggtgtcc ggcggcgcga cggacgtgag
     1261 cgagagcagc ggtgagccgg gcaccgggcc gggcacgacc ggcgcgccgg acacgggcga
     1321 ggactcgacg agcgaggcga ccagcggcgt gagcacgacc ggcgacacga gcacgggcgg
     1381 ggacagcgag tcggggatgt cgacgggggt cggcgagggc ggctgcgccg acgggacgca
     1441 gggctgcccg tgctacggca acatgacgtg caacgacggg ctcgcgtgcg agggcgacgt
     1501 gtgcgtgccg atgcaggcgg cggtgtgtgg cgacggcatg ctcggcgggg ccgagcagtg
     1561 cgacgccggg gtggagaacg gcgacaccaa gctgtgcaag tcggactgca ccgcgcaaaa
     1621 gtgcggcgat gggttcgtcg gccccggcga ggcgtgcgac gacggcaacg cggtcgacga
     1681 cgacgcgtgc tcgaacgcct gcgtgccggc ggcgtgcggc gaccaggtgg tgcaggtggg
     1741 cgaggcgtgc gacgacggca acgcggaccc caacgacggg tgcatcacgt gcaaggcggc
     1801 ggcgtgcggc gacaccttcg tgcaggcggg ggtcgaggac tgcgacgacg gcaacgtcaa
     1861 cgacggtgat ggttgcagcg cggcgtgcaa gaaggaggcg cccaagtgcg gcggcaactt
     1921 cacgaccgac tggtgcccgc aggcggggac gaaggagcag ttcacgcgct gcgagagcgt
     1981 cagcaacggc ggtaagacct gcaacaaccc gttcgtgaag tacggatcga tcgacaacgg
     2041 ggtgccggcg agccacgaca gcaacgactt caacgcgtgg tgcacacagc tgggcttcgc
     2101 cggcttcagc ggccaggtca gctacggcag ccgcccgtgc gacgcgccgc agggcaagct
     2161 gttcggctgc accggctggg acgagaacgt gtggcactgg tgcgactggc aggacgggta
     2221 ctggtacagc cagatcctcg actggcatag ttgcaacgac ggccaggaga tcacgtcgat
     2281 cacctgtcag tgagacgatg atcgtcgtca gtcaggcgcc gatgccgttg gcggaggggg
     2341 gcgacgacat cgtcgcctcg acgcgcgccg cggtggcggc gggcttcgag ctggtgacgc
     2401 cgcggtgtgg gtcggcacga tggcgcacga ctacgactga ccgtatcgcg gcgagtgggc
     2461 gacgctcgac gaggccgagg cggcggcgat gctggcggtc gcgagcgcgg cggcgcggcg
     2521 gctgcgggcg cggctggtgt cggtcgacat cggacagctc gaggacgggg actgggtcgt
     2581 gatcgaggtc ggagatccgc agttctcggg gctgagcttc atccgcccgc ggacgctgtg
     2641 ggaggcgctg gcggaccggc tgggttgagc gcgtgcagct gcaactgcgc ggcggcgagg
     2701 cgcaggtcct cgcgcaggcc ggcgagcgcg cgctcgcggt ccgcgggggt gagcgatcgc
     2761 agggcg
//
LOCUS       DASWNJ010000714         1785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_296490, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000714 DASWNJ010000000
VERSION     DASWNJ010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296490"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="VGB85_11900"
     CDS             complement(<1..1127)
                     /locus_tag="VGB85_11900"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY0134782.1"
                     /translation="MEPETSTSTTDLPTTGDDTVGESSSTGDEDTTTGDDLPHPELYP
                     TDRVQSPITAFVAAKIREIAAAPAGADKDPGVFAKIGGGSTASANFMNCLADEMMIQD
                     LPMDEAALKTTIDHFRAVALVDMTTSFNRASVAAMNGFTSDALVTMPTPIAMELEAIK
                     PRFAQVLVGTHDLDKDQPAQLWVFAEKMLDMVDALTINGVVPILTTIPQRTDIPAKDL
                     FVPRYNAVLRAVAQGRQIPLVDLNLALAGLPMSGVAAEGDLSVFVSAMVDRPCHFGEA
                     ALMYGYNVRNLEALRSLDRARQVVVEEVPELDTPGPRLAGAGTMADPYQIPSLPFVDL
                     RSTADSTSDIIDNYLGACDGMKDESGPEVFYKLEVEAPIDVR"
BASE COUNT          240 a          615 c          640 g          290 t
ORIGIN      
        1 cggacgtcga tcggggcctc gacctcgagc ttgtaaaaca cctcggggcc ggactcgtcc
       61 ttcatgccgt cgcaggcgcc gaggtagttg tcgatgatgt cgctggtgct gtcggcggtg
      121 ctgcgcaggt cgacgaaggg cagcgagggg atctggtacg ggtcggccat cgtgccggcg
      181 cccgcgaggc gcggaccggg cgtatcgagc tcgggcacct cctcgacgac gacctgacgc
      241 gcgcggtcga gggaccgcag ggcctcgagg ttgcgcacgt tgtagccgta catcagggcg
      301 gcctcgccga agtggcacgg gcggtcgacc atcgccgaca cgaacaccga cagatcgccc
      361 tcggcggcca cgccggacat cggcaggccg gccagcgcga ggttgaggtc gacgagcggg
      421 atctggcggc cctgggcgac cgcgcgcagc accgcgttgt agcgcggcac gaacaggtcc
      481 ttggccggga tgtcggtgcg ctgcgggatc gtcgtcagga tcgggaccac gccgttgatc
      541 gtcagcgcgt cgaccatgtc gagcatcttc tcggcgaaca cccacagctg cgcgggctgg
      601 tccttgtcga ggtcgtgcgt gccgacgagc acctgcgcga agcggggctt gatcgcctcg
      661 agctccatgg cgatcggcgt cggcatcgtg accagcgcgt ccgacgtgaa gccgttcatc
      721 gccgcgacgc tcgcgcggtt gaaggaggtc gtcatgtcga cgagcgcgac cgcgcggaag
      781 tggtcgatcg tcgtcttcag cgccgcctcg tccatgggga ggtcctggat catcatctcg
      841 tccgcgaggc agttcatgaa gttggcgctg gccgtcgagc cgccgccgat cttcgcgaac
      901 accccgggat ccttgtccgc gcccgcgggt gccgccgcga tctcgcggat cttggcggcg
      961 acgaacgccg tgatcggcga ctgcacgcgg tcggtcgggt acagctccgg gtgcggcagg
     1021 tcatcgccgg tggtcgtgtc ctcgtcgcca gtgctgctgc tctcgccgac cgtgtcgtcg
     1081 ccggtggtcg gcagatcggt cgtcgaggtc gaggtctcgg gctccacggt cgtctcggtc
     1141 gcgggtggcg cttcggtcgt ctctgtcgcg ccggagctgc cccccgagct gccgctgttg
     1201 ccgtccgtcg aaccctcgcc gaagccgact ccactggcgc aagcgggcgc gcacgccagc
     1261 gcgaacaaca gggacgcggc gcgccctttc gtgccacaaa acataggcgc acaaggtaca
     1321 cgatccaccc gcgcggccgc ccccgggaag ttcgcgcagg cgggcgtctc cgcccgcgtg
     1381 taggttgatg tggcgggcaa aggcgcgccg tgacggcctt gttctccggt gccgcgtggg
     1441 ccggtgcggc cgcgcccgcg ccccgggacc cgcacgagga tcaccgcgca aagatcaccg
     1501 gcgcgggcgc ggcggtcccg gctcatcggc ggtccgcccc gggcccgatg acctgtcctt
     1561 ggggacatgt cctcgcggcc tgaccagatg tccttcggta catgtctctg acgccatgcg
     1621 agtttgcggg ccacgttcgg ctgcccgcgc gatgacgcag acctaaccat gttcacctcg
     1681 cgacctcgcg caccgtcgcg cctgcgtcga ggggtcgcgt cgagcatgcg ccgacgctgg
     1741 cgcgtggcat actcgcggcc gtgaaacgca cctcgctgtc tcgcc
//
LOCUS       DASWNJ010000715         1617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_472056, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000715 DASWNJ010000000
VERSION     DASWNJ010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472056"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /gene="ftsZ"
                     /locus_tag="VGB85_11905"
     CDS             complement(<1..1153)
                     /gene="ftsZ"
                     /locus_tag="VGB85_11905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY0134783.1"
                     /translation="MRLKPPAGGPVNRVFFPAPHTQQRHLPALVRARGRRATERYIAV
                     AAAQNYLAIIKVVGIGGGGVNAVNRMIEVGLRGVEFIAINTDAQALLMSDADVKLDIG
                     RELTRGLGAGANPDVGARAAEDHAEEIEEVIKGADMVFVTAGEGGGTGTGGAPVVARI
                     ARSLGALTIGVVTRPFAFEGRRRANAAEEGIAGLREEVDTLIVIPNDRLLSISDRSVS
                     VLDAFKQADQVLLQGVSGITDLITTPGLINLDFADVKSVMANAGSALMGIGSARGEDR
                     AVAAAEMAVSSPLLEASIEGAMGVLLSIAGGSDLGLFEINEAAALVAQSAHGEANIIF
                     GATIDDALGDEVRVTVIAAGFENGMPKRRDDGTVLRREPRPQVTQAEVRE"
     gene            complement(1266..>1617)
                     /locus_tag="VGB85_11910"
     CDS             complement(1266..>1617)
                     /locus_tag="VGB85_11910"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEY0134784.1"
                     /translation="DAAGVVFRDYPRPPRTLPLVRTTGRTGGEALSEAARVISALPGA
                     LARRVEYLAVLTVDQITLVLRDGRQVVWGSAEESPAKAEVLAALLGQPAKVYDVSVPG
                     QPTTSQQAPSTPLP"
BASE COUNT          213 a          567 c          588 g          249 t
ORIGIN      
        1 cctcccggac ctcggcctgg gtgacctggg gccgaggctc gcggcgcagc accgtgccgt
       61 cgtcgcgccg cttcggcatg ccgttctcga acccggcagc gatgacggtg acccggacct
      121 cgtcgccgag cgcgtcgtcg atggtcgccc cgaagatgat gttggcctca ccgtgggcgg
      181 actgcgcgac gagtgcggca gcctcgttga tctcgaagag tccgaggtcg gacccgccgg
      241 cgatcgagag caggacgccc atggcgcctt cgatggatgc ctcgagcagc gggctggaga
      301 cggccatctc ggcggcggcc accgcgcggt cctcaccgcg cgcggagccg atccccatca
      361 gcgcggagcc ggcgttggcc atcaccgact tcacgtcggc gaagtcgagg ttgatcagcc
      421 ccggcgtggt gatgaggtcg gtgattcccg agacaccctg gagcagcacc tggtcggcct
      481 gcttgaaggc gtcgagcacc gagacgctcc ggtcgctgat ggagagcagc cggtcgttcg
      541 ggatgacgat cagggtgtcg acctcctcgc ggagcccggc gatgccctcc tcggcggcgt
      601 tggcacgccg gcggccctcg aaggcgaacg gccgggtcac gaccccgatg gtcagcgcgc
      661 ccagcgagcg ggcgatgcgg gcgacgaccg gcgcgccgcc ggtgccggtg ccgccgccct
      721 cgcccgccgt cacgaagacc atgtcggccc ccttgatgac ctcctcgatc tcctcggcgt
      781 ggtcctcggc ggcgcgggcc cccacgtcgg ggttggcccc cgcgccgagt ccgcgcgtga
      841 gctcgcggcc gatgtcgagc ttgacgtcgg cgtcgctcat caggagcgcc tgtgcatcgg
      901 tgttgatcgc gatgaactcg acgccccgga ggccgacctc gatcatccgg ttgacggcgt
      961 tcacgccgcc gccaccgatc cccacgacct tgatgatggc caggtagttc tgggctgctg
     1021 ccacggcgat gtacctttcg gttgctcggc gcccgcgggc gcggacgagg gctgggaggt
     1081 gtctctgctg ggtgtgcggt gcggggaaga acacccggtt gaccggccct ccggctggcg
     1141 gtttcagtct caccgtcaac ctgagggtta acgttatgtc aacctgatgt tggtcacgac
     1201 agtaggcagg ggcctacccc gagtccagca ccggccgggc gcgtcgcgcc tttcttcgca
     1261 ggagctcagg gcagcggcgt ggacggcgcc tgctgcgacg tcgtgggctg tcccgggacg
     1321 ctcacgtcgt agaccttcgc gggctggccg aggagcgccg ccagcacctc ggccttcgcc
     1381 ggggactcct ccgcgctgcc ccacaccacc tggcgcccgt ctcgcagcac cagggtgatc
     1441 tggtcgacgg tgagcacggc gaggtactcc acccttctcg ccagggcgcc cggcagtgcg
     1501 gagatgacgc gggcggcctc ggagagcgcc tccccgcccg tgcggccggt ggtccgcacg
     1561 agcggcaggg tgcgaggagg ccgggggtag tcgcggaaga cgacgccggc ggcgtcc
//
LOCUS       DASWNJ010000716         1748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_438120, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000716 DASWNJ010000000
VERSION     DASWNJ010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438120"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VGB85_11915"
     CDS             <1..861
                     /locus_tag="VGB85_11915"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY0134785.1"
                     /translation="VGYRDEASWHALLDEEIAEARTRVQAGTARADLHASFQAEAVHA
                     PIELDAAAEAARQELAGRFAEDLSQLPSDFVEAEPGRRYPVPEDPGAPTLGPASAPVT
                     LVAFMDFECPYCRRAAEALKDLPRRYPADLRIVFRHLPLPIHRAAEGAARASIAAGLQ
                     GQFWPYSERLLAPEVRGLGRSTFLDVARALGLDEARFLADLDGPVAAARVRDDMLLAR
                     RLGLDGTPAFLLNGRYVSGYRGVDALAADIDAELATAKTLLAEGIPPAALVDTLRTRE
                     GSPLPNPDSP"
     gene            879..>1748
                     /locus_tag="VGB85_11920"
     CDS             879..>1748
                     /locus_tag="VGB85_11920"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY0134786.1"
                     /translation="MKTISSIFLVTALALTTTSLAGCTGNRGGQKADATAEAAPTGAS
                     KIITMEQVDDSLNAKIQGERFRVTYGDQDPYTGAEAPLVTIVEFSDFQCPFCSKFGDM
                     LHEVAKDPAYANDLRIVFKQFPLPMHKDAQMGAEAALAANEQGKFWEMHDLLFKNQKA
                     MTRADVEKYAEELKLDMAKFKEALDKGTYKTKVTADLDMGKQFGVRGTPSFFINGKWQ
                     RGAPRAIEGLKKLIDEEKAAAEQLIKDGSKREEIYARIMKAAKDKRTEPPAENKPRPG
                     APDPAVNYAVPADG"
BASE COUNT          294 a          676 c          554 g          224 t
ORIGIN      
        1 gtcggttacc gcgacgaggc cagctggcac gccctgctcg acgaggagat cgccgaggca
       61 cgcacccgcg tgcaggccgg tacggcgcgg gcagatctcc acgcctcgtt ccaggccgag
      121 gccgtgcacg ccccgatcga gctcgacgcg gcggccgagg ccgcgcgcca ggagctcgcc
      181 ggtcgcttcg ccgaggacct gagccagctc ccctcggact tcgtcgaggc cgagcccggc
      241 cgccgctacc cggtccccga ggacccgggc gcccccacgc tcggcccggc gagcgccccg
      301 gtgacgctcg tcgccttcat ggacttcgag tgtccttact gccgccgcgc cgccgaggcc
      361 ctcaaggatc tgccgcgcag gtaccccgcc gatctccgga tcgtgttccg tcacctcccg
      421 ctgccgatcc accgcgccgc cgaaggagcc gcgcgggcct cgatcgccgc cggtctccag
      481 ggccagttct ggccgtacag cgagcgcctg ctcgccccgg aggtgcgagg cctcggccgc
      541 agcaccttcc tcgatgtcgc ccgcgcgctc ggcctcgacg aggcccgctt cctcgccgac
      601 ctcgacggcc ccgtcgccgc ggcgcgcgtg cgtgacgaca tgttgctcgc tcgccggctc
      661 ggcctcgacg gcacgccggc gttcctcctc aacggccgct atgtcagcgg ctaccgcggc
      721 gtcgacgctc tggccgccga catcgacgcc gagctcgcga cggccaaaac cctgctggcc
      781 gagggcatcc cgcccgccgc gctggtcgac accctgcgca cgcgcgaggg cagcccgctc
      841 ccgaaccctg attcgccttg aaccgagaag acaccatcat gaagaccatc agctctattt
      901 tcctcgtcac ggcattggcc ctgaccacca cctctctggc cggctgcacc ggcaaccgcg
      961 gcggccagaa ggccgacgcg acggcggagg ccgctccgac cggcgccagc aagatcatca
     1021 ccatggaaca ggtcgatgac tcgctcaacg ccaagatcca gggtgagcgc ttccgcgtca
     1081 cctacggcga ccaggacccg tacaccggcg ccgaggcccc gctcgtcacg atcgtcgagt
     1141 tctccgactt ccagtgcccg ttctgctcca agttcggcga catgctgcac gaggtcgcca
     1201 aggacccggc ctacgccaac gacctgcgca tcgtcttcaa gcagttcccg ctgccgatgc
     1261 acaaggacgc ccagatgggc gccgaggcgg cgctggccgc caacgagcag ggcaagttct
     1321 gggagatgca cgacctgctc ttcaagaacc agaaggcgat gacccgcgcc gacgtcgaga
     1381 agtacgccga ggagctcaag ctcgacatgg ccaagttcaa ggaggcgctc gacaagggca
     1441 cctacaagac caaggtcacc gccgacctcg acatgggcaa gcagttcggc gtgcgcggca
     1501 ccccgagctt cttcatcaac ggcaagtggc agcgcggcgc gccgcgtgcg atcgaggggc
     1561 tcaagaagct gatcgacgag gagaaggccg ccgccgagca gctgatcaag gacggctcca
     1621 agcgcgagga gatctacgcg cgcatcatga aggccgccaa ggacaagcgc accgagccgc
     1681 cggcggagaa caagccgcgc cccggcgcgc ccgacccggc cgtcaactac gccgtgcccg
     1741 ccgacggc
//
LOCUS       DASWNJ010000717         3193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_365522, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000717 DASWNJ010000000
VERSION     DASWNJ010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365522"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VGB85_11925"
     CDS             complement(<1..816)
                     /locus_tag="VGB85_11925"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0134787.1"
                     /translation="MVERLHEGPATIVYRGRRDADGSTVALKILRGDPPSPADVEQLR
                     REHALLAGLAVPGLPRVFGLETLDGRLCLVMEALPGRPLDEVLATGRLPLATVLRLGV
                     VLAGVIGRLHLAGVIHKDIKPANIVVDLDAGEAMLVDLGVAALRSEAPLREGEIEGTL
                     AYLSPEQTGRMNRRVDHRADLYSFGVTLYEMVTGALPFTSTDPLEIVHSHVARAPTPP
                     HARDAPQVVSDIIVKLLAKAAEDRYQSAFGLQADLESCLESLHRTGRVEPFVLG"
     gene            1134..2075
                     /locus_tag="VGB85_11930"
     CDS             1134..2075
                     /locus_tag="VGB85_11930"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0134788.1"
                     /translation="MRATDFLFLPLLLTSLVGCVESPEAEPRNLSVASGPLTPTRYLT
                     IDGLQIAVYEGPGNKGPGVLLLHGNTSSANAFARILKASALTKRRVVAIDLPGYGNSD
                     DAEYDIATFTSVIAQAAVELGVDDGVMVGWSLGGDFLLQTAHLLPDVRGYFLFGTAPV
                     GGGPPISPFLGPDESYAGAATQYGFVADLSEAQIDEYVHAFFRPGWWNIPQFFFDDGY
                     RTDPGTRQAVLEAASGLDPDFLAEVPVAQALTVPIALVHAEDDAFVRLDYLKAIAPSI
                     PNLWQDKIIVVPSSGHALQWERPITFGILLEAFLADL"
     gene            complement(2097..2732)
                     /locus_tag="VGB85_11935"
     CDS             complement(2097..2732)
                     /locus_tag="VGB85_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134789.1"
                     /translation="MRDLEKLRRAAEALDRDTSITLLTSHDQAPEGVSRVGGPGFDLG
                     DRQPQGEEQPMTHLWTLATADVPALANAFPGAAAVALYMADPEENEAYEAFNGLTALV
                     PVSAEDIARGEAEPTGQDLGPRHVTAEHLEVASSAFVYDSEDEDASPRRVLRNAIYGA
                     SARAGGGPIWLQGDEHEGDFLLQFDESFASVNLGDCGIMYVFTDTQFWQCH"
     gene            complement(2753..>3193)
                     /locus_tag="VGB85_11940"
     CDS             complement(2753..>3193)
                     /locus_tag="VGB85_11940"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEY0134790.1"
                     /translation="LLGDELWTETAILLQKIADLAPAAGLAPPRTDPRRARFDERLHF
                     IATELHKGFAPFTLMPNVGEDSKRWAAERLAQRVGLLAAELGDRPYLDGDRFTVADAY
                     AFWALRTYSRLLRAELPSPLTAYLDRVGARPAVREALAAEGITP"
BASE COUNT          445 a         1147 c         1109 g          492 t
ORIGIN      
        1 accgagcacg aagggctcga cgcggccggt cctgtgcagg ctctccaggc acgactcgag
       61 gtcggcctgc agcccgaacg cgctctggta gcggtcctcg gccgccttcg ccagcagctt
      121 gacgatgatg tccgagacga cctgcggggc atcacgggcg tgcggcggcg tcggtgcgcg
      181 cgcgacgtgg ctgtgcacga tctccagggg atccgtcgag gtgaacggca gcgcgccggt
      241 caccatctcg tagagcgtca cgccgaacga gtagaggtcc gcgcggtggt cgacgcggcg
      301 gttcatgcga ccggtctgct cgggcgacag gtacgcgagc gtgccctcga tctcgccctc
      361 gcgcagcggc gcctcggacc gcagcgcggc gaccccgagg tcgacgagca tcgcctcgcc
      421 ggcgtcgagg tcgacgacga tgttggccgg cttgatgtcc ttgtggatca cgcccgcgag
      481 gtgcagccgg ccgatcacgc ccgcgagcac gaccccgagc cgcagcacgg tcgccagcgg
      541 cagccgcccg gtcgcgagca cctcgtcgag cggccgtccg ggcagcgcct ccatcaccag
      601 acagagccgg ccgtcgagcg tctcgaggcc gaagacccgc ggcagccccg gcaccgcgag
      661 gcccgcgagc agcgcgtgct cgcgacgcag ctgctcgaca tccgcggggc tcggcgggtc
      721 gccgcgcagg atcttgagcg ccaccgtcga gccgtccgcg tcgcgtcgac cgcgatagac
      781 gatcgtcgcg ggaccctcgt ggagcctctc caccatcgtg tatcccggca gcttcaacat
      841 tacaggacga ggcaatcgga catcgcgacg cccgtcaacc ctcgccgtcg aaagttttgg
      901 ccgaaccgtc cctgtccgct gcggacatgt atccggacgc ggttgtcctc gcgggatcgg
      961 tctcgagccc gcgcatgcgg agcgccgcgt cggccggaca tacacaggcc caccactgcg
     1021 ccgcggcgcg ctggtggaag tcctcttggc ggcgcctggc gacttgagca atattcgctg
     1081 aaagccgctg cacgggcggg cttcgtccct cttgcacccg aggtcgccga cacatgcgag
     1141 ccactgactt cctgttcttg ccgctcctcc tgaccagcct cgtcggctgt gtcgagtcgc
     1201 ccgaggccga gcctcgcaat ttgagcgtcg cctcgggccc gctgacgccg acgcgttacc
     1261 tgacgatcga cggcctgcaa attgcggtct acgagggccc gggcaacaag ggtcccggcg
     1321 tgttgctgct gcacggcaac acctcctctg cgaatgcgtt cgcgcggatc ttgaaggcga
     1381 gcgcgctgac caagcggcgc gtcgtggcga tcgacctccc ggggtacggc aactcggacg
     1441 acgccgagta cgacatcgcg actttcacca gcgtcatcgc ccaggcggcg gtcgagctcg
     1501 gggtcgacga cggcgtgatg gtcggctgga gcctcggcgg cgacttcctg ctgcagaccg
     1561 cgcacctgct gccggacgtg cgcggctact tcctgttcgg tacggcgccg gtcggcggtg
     1621 gcccgccgat ctctccgttc ctcggtcccg acgagagcta cgccggcgcg gcgacccagt
     1681 atggttttgt cgccgacctc agcgaggcgc agatcgacga gtatgtgcac gccttcttcc
     1741 ggcctggctg gtggaacatc ccgcagttct tcttcgacga cggctaccgg accgacccgg
     1801 gcacgcgcca ggcggtgctc gaggccgcct cggggctcga cccggacttc ctcgccgagg
     1861 tgccggtcgc ccaggcgttg acggtcccga tcgcgctcgt gcacgcggag gacgacgcgt
     1921 tcgtgcgcct cgattatctg aaggcgatcg ccccgagcat cccgaacctg tggcaggaca
     1981 agatcatcgt cgtgccctcg tcgggccacg cgctccagtg ggagcgcccg atcaccttcg
     2041 gcatcctgct cgaggccttc ctggccgacc tgtgaggcgg ccaggctggc ggtgcgtcag
     2101 tggcactgcc agaattgcgt gtccgtgaac acgtacatga tcccgcagtc gccgaggttg
     2161 acgctggcga acgactcgtc gaactgcagc aggaagtcgc cctcgtgctc gtcgccctgc
     2221 agccagatcg gcccgccgcc ggcccgcgcc gacgccccgt agatcgcgtt gcgcaggacc
     2281 cggcgtggcg acgcgtcctc gtcctcggag tcgtagacga aggccgacga cgcgacctcg
     2341 aggtgttcgg ccgtgacgtg gcgcgggccg aggtcctgcc cggtcggctc ggcctcgccg
     2401 cgcgcgatgt cctccgccga caccggcacc agcgcggtca gcccgttgaa cgcctcgtag
     2461 gcctcgttct cctccgggtc cgccatgtac agcgcgaccg cggccgcccc cgggaacgcg
     2521 ttcgccagcg caggcacgtc ggcggtcgcc agcgtccaca gatgggtcat cggctgctcc
     2581 tcgccttgtg gctggcggtc gccgaggtcg aagcctggcc cgccgacccg cgacacgccc
     2641 tcgggcgcct ggtcgtgtga ggtgagcagc gtgatgctcg tgtcccggtc cagcgcctct
     2701 gcggcgcgac gaagcttctc gaggtctctc atgcggcgga cgatacaccg atttacgggg
     2761 tgatgccctc ggccgcgagc gcctcacgca cggccggccg ggcgccgacg cggtcgaggt
     2821 acgccgtgag cggcgacggc agctcggcac gcaacaggcg gctatatgtc cgcagcgccc
     2881 agaaggcgta cgcgtcggcg acggtgaagc ggtccccgtc gaggtagggc cggtcgccga
     2941 gctccgcggc gagcaggccg acgcgctgcg cgagccgctc ggcggcccat cgcttgctgt
     3001 cctcgccgac gttgggcatc agggtgaacg gcgcgaagcc cttgtgcagc tcggtggcga
     3061 tgaagtgcag gcgctcgtcg aagcgggccc gtcgcgggtc ggtccgcggc ggcgccagcc
     3121 cggccgccgg cgcgaggtcg gcgatcttct gcagcaagat cgccgtctcg gtccacagct
     3181 cgtcgccgag cag
//
LOCUS       DASWNJ010000718         1726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_154377, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000718 DASWNJ010000000
VERSION     DASWNJ010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154377"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VGB85_11945"
     CDS             <1..436
                     /locus_tag="VGB85_11945"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HEY0134791.1"
                     /translation="YFDRATVERERPLVARAGDLNVSAHYLIDRDGTIVRLMPDTFMA
                     RHVVGLNHLAIGVENVGDGERFPLTPAQLAANVALIRDLKARFPGITHVIGHHEYRAL
                     AGHPYFKERQRGHRTRRDDPGRKFMNAVRSALADLDLEGPPP"
     gene            503..1438
                     /locus_tag="VGB85_11950"
     CDS             503..1438
                     /locus_tag="VGB85_11950"
                     /inference="COORDINATES: protein motif:HMM:NF021631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY0134792.1"
                     /translation="MQSPGTLIYETVHGSQAYGLATPTSDVDLKGVLVGPRAWYHGYL
                     GGPEQVELTGDHVRYEIRKYFRLAAAANPTVLELLWTPVECHVHMTPAGERLLAARDG
                     FLSLRVKDSFSGYAMSQLGRIKTHRKWVMHPPTREPSRSDFGLSDRAQISRDQMGAVE
                     AMLADGRLEQVDLSTNFLDLLEKERRYRTARKEWESYQSWQKTRNPRRSELEAKFGYD
                     TKHALHLIRLLRMGLEIVATGRVIVRRDDREELLGIKAGALAYDELMERAEALGKQVH
                     AAAAASPLPPAPDDAALDRLCAELVDDVLRAPESP"
     gene            1438..>1726
                     /locus_tag="VGB85_11955"
     CDS             1438..>1726
                     /locus_tag="VGB85_11955"
                     /inference="COORDINATES: protein motif:HMM:NF021631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY0134793.1"
                     /translation="MTATDLTVLPPNALPDLELGRRFIAASPVPGAVLQCGVTGSHDY
                     GFSSPDSDLDLKGLFVAPTRRLLGMHRPPEAFDRLIDFEGVECDLTLTEVGK"
BASE COUNT          254 a          692 c          545 g          235 t
ORIGIN      
        1 ctacttcgac cgcgccaccg tcgagcgcga gcgcccgctc gtcgcccgcg cgggcgacct
       61 caacgtctcg gcgcactacc tgatcgaccg tgacggcacg atcgtgcggc tgatgcccga
      121 caccttcatg gcgcgccacg tcgtcggcct caaccacctc gcgatcggcg tcgagaacgt
      181 cggcgacggc gagcgcttcc cgctgacgcc cgcgcagctc gccgcgaacg tcgcgctgat
      241 ccgcgacctc aaggcccgct tccccggcat cacccacgtg atcggccacc acgagtaccg
      301 cgccctcgcg ggccacccgt acttcaagga gcgccagcgc ggccaccgga cccgccgcga
      361 cgaccccggt cgcaagttca tgaacgccgt acgctccgcg ctcgccgacc tcgatctcga
      421 gggtccgccg ccatgacccg ctgacacgcg cgcgcccgca cgtgcacatg cacggcgacg
      481 ccgtgctacc gtggcgcccg ccatgcagtc tcccggcacc ctgatctacg agaccgtgca
      541 cggctcgcag gcctacggcc tggcgacgcc gacctcggac gtcgacctca agggcgtgct
      601 cgtgggcccg cgcgcctggt accacggcta tctcggcggc ccggagcagg tcgagctgac
      661 cggcgatcac gtgcgctacg agatccgcaa gtacttccgc ctcgccgccg ccgccaaccc
      721 gaccgtgctc gagctgctgt ggaccccggt cgagtgccac gtgcacatga cccccgccgg
      781 cgagcggttg ctcgccgcgc gcgacggctt tttgtcgctg cgggtcaagg acagcttctc
      841 cggctacgcg atgtcgcagc tcggccggat caagacccac cgcaagtggg tgatgcaccc
      901 gccgacgcgc gagccctcgc gctccgactt cggcctgtcc gaccgcgccc agatctcgcg
      961 cgaccagatg ggcgcggtcg aggcgatgct cgccgacggt cggctcgagc aggtcgacct
     1021 gagcaccaac ttcctcgacc tgctcgagaa ggagcgccgc tatcgcaccg cgcgcaagga
     1081 gtgggagtcg taccagtcgt ggcaaaagac gcggaacccg cggcgctccg agctcgaggc
     1141 caaattcggc tacgacacca agcacgcgct gcacctgatc cgcctgctgc gcatgggcct
     1201 cgagatcgtc gccaccggcc gcgtgatcgt ccgtcgtgac gaccgcgagg agctgctcgg
     1261 catcaaggcc ggcgcgctcg cctacgacga gctgatggag agggccgagg cgctcggcaa
     1321 gcaggtccac gccgccgctg ccgcctcgcc gctgcccccc gcccccgacg acgccgccct
     1381 cgatcgcctg tgcgccgagc tcgtcgacga cgtgctgcgc gccccggaga gcccgtgatg
     1441 accgccaccg acctgaccgt gttgcccccg aacgccctgc ccgacctcga gctcgggcgc
     1501 cgcttcatcg ccgccagccc ggtgcccggc gccgtcctcc agtgcggcgt caccggcagc
     1561 cacgactacg gcttctcctc gcccgacagc gacctcgacc tcaagggcct gttcgtcgcg
     1621 ccgacgcgcc ggctgctcgg catgcaccgc ccgcccgagg ccttcgatcg cctgatcgac
     1681 ttcgagggcg tcgagtgcga cctgacgctg acggaggtcg gcaagg
//
LOCUS       DASWNJ010000719         3971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_543505, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000719 DASWNJ010000000
VERSION     DASWNJ010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543505"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..927)
                     /locus_tag="VGB85_11960"
     CDS             complement(235..927)
                     /locus_tag="VGB85_11960"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0134794.1"
                     /translation="MTNTLITGANKGLGFETARRLIALGHTVFLGARDRGRGEAAAQR
                     LGARFVQLDVTDDASVTAAAAWIEQQGDLDVLINNAGISGAFIAAPDVTADAMREVYE
                     TNVFGIVRVTHAMLPLLRASSNPVIVNVASGLGSFGVVHDPDRIESTVTALAYCSSKS
                     AVTMLTVQYARALPELRINVVDPGYTATDLNHHSGPQTVEQGTDAIVRLATIGKDGPT
                     GSFQDAAGLLPW"
     gene            complement(924..1145)
                     /locus_tag="VGB85_11965"
     CDS             complement(924..1145)
                     /locus_tag="VGB85_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134795.1"
                     /translation="MACKALINGSPAHASLHAVSTLASHRPVTAANVHALASEKVTTF
                     THAQRIATDRRPFIYSLVHASRSPYPGLP"
     gene            complement(1356..1802)
                     /locus_tag="VGB85_11970"
     CDS             complement(1356..1802)
                     /locus_tag="VGB85_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134796.1"
                     /translation="MLQRSQVLARLTWVVLALVLIGVLGGLHPSDRTRDLVVAAAAIL
                     VALLNAPELIDGLRTRGTTVVLRAASRLVAAACVLLFVRSISHEQRWDAKDAYRRCAG
                     PDARWPDDPAQRCRVLHMCINEAAPTPGEHVRLRDLIAATPGCPPP"
     gene            complement(1804..2040)
                     /locus_tag="VGB85_11975"
     CDS             complement(1804..2040)
                     /locus_tag="VGB85_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134797.1"
                     /translation="MNAIEASEIFALEPGASKTMSTWIAGPSYGPGRYEVRLRYINDP
                     GKRESANTSPEVTAMLAKTSACDVTSAPLAIKVP"
     gene            complement(2040..2456)
                     /locus_tag="VGB85_11980"
     CDS             complement(2040..2456)
                     /locus_tag="VGB85_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134798.1"
                     /translation="MRPTASLATALAVLLPIACAAPVPVDPPSPAVTLASPPAPPAVA
                     QASEPAASIPVAPRPVEPAGEQLDDCGLRATIASKRGPDGDTQYVLTLKNEGTTARTL
                     VTPGDGSEHGRRTPLLSWSGTRDDKPAPQLERRAAA"
     gene            2903..3706
                     /locus_tag="VGB85_11985"
     CDS             2903..3706
                     /locus_tag="VGB85_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134799.1"
                     /translation="MKNLVILSLPLCLLTGLVVGCDAEPESTADLAPSSSAAAQGMWP
                     LPLPAVAAQPRSSGQKSVKAKHLIAKDAQATAWTRWVMALPYSTGPINDPTGAACASS
                     QAGDLWFLAGTAGGSAERTCDVPAGKQLVFPLLNWFCAFFPEIYPDEQAVAQGVSEMV
                     AAVASLPDGVCELTLKIDGVDVLADLDAANELFGFTDEAFDVEANDDHFASSEGFAGG
                     TIPAITAGYYVRLKPLAPGDHVLEFGGALCTDGKVDFSTHTYYNLHVGM"
BASE COUNT          597 a         1331 c         1438 g          605 t
ORIGIN      
        1 gaggcggact tatcgatcat cgggtcgaca ggatcctgca tcgtcgtgaa gctcgccgcc
       61 atctccggca ccccagcagg tggcgtgcca gccagtttcc cttggagatc cggggcgccg
      121 atcacgaacc tttgtcacaa tggcgcgccc cggcgccgac gcatgatgtc ccgtcatcac
      181 gacccggtgc gggcgccctg gcgcgacgcc gacgacaccg ggtcggatgc gcagtcacca
      241 cggcagcagt ccggccgcgt cctggaagct gccggtcggg ccgtccttgc cgatggtcgc
      301 caggcggacg atcgcgtcgg tgccctgctc gacggtctgc ggcccggagt gatgattgag
      361 gtcggtcgcg gtgtacccgg ggtcgacgac gttgatgcgc aactcgggca acgccctcgc
      421 gtactggacg gtcaacatcg tgacggcgga cttcgacgag cagtacgcga gcgcggtgac
      481 agtggactcg atccggtcgg ggtcgtgcac caccccgaac gagccgagcc cgctggcgac
      541 gttgacgatg accgggttgc tcgaggcgcg cagcagcggc agcatggcgt gggtcacgcg
      601 gacgatgccg aagacgttgg tctcgtagac ctcgcgcatc gcgtcggcgg tgacgtccgg
      661 cgcggcgatg aaggcgcccg agatcccggc gttgttgatc aggacgtcca ggtcgccctg
      721 ctgctcgatc cacgcggccg cggccgtgac cgaggcgtcg tcggtgacgt cgagctgcac
      781 gaagcgcgcg cccaggcgct gggccgcggc ctcgccccgg ccccggtcgc gcgcgccgag
      841 gaagacggta tgcccgaggg caatgagacg acgcgccgtt tcgaagccga ggcccttgtt
      901 ggcgccggtg atgagggtgt tggtcatggg aggccagggt aaggggagcg cgatgcatga
      961 accagcgaat agataaatgg ccgccggtcg gtggcaatcc gctgcgcatg ggtgaaggtc
     1021 gtgaccttct ccgacgccag cgcatgtacg ttggccgcgg tcacgggtcg atgcgaggcc
     1081 agcgtcgaca ccgcgtgcag cgacgcgtgc gccggcgagc cgttgatcag cgccttgcag
     1141 gccatcccca gcgagctcga gatcgtaaaa atgtgcggcg gcccgatcgg tccgtgggcc
     1201 gcggtgggca gcgaacccgg cgcatacgtg ggcgcctgcg ccagccacct cacaccgcct
     1261 gcgccgccgc tgtgcgactg tgccgcggtc gcctgcgccg ccgaatgagc cgtcaaagcg
     1321 gcattgtggt cagaccggcg agggggcgcg acgcttcacg gcggcgggca gcccggcgtc
     1381 gctgcgatca ggtcgcgcag gcggacgtgc tcacccgggg tcggcgcggc ctcgttgatg
     1441 cacatatgca gcacgcggca gcgctgcgcc ggatcgtccg gccagcgcgc gtccgggccg
     1501 gcgcaccgac gatatgcatc cttggcgtcc cagcgctgct cgtgcgagat gctgcggacg
     1561 aacagcagga cgcacgcggc cgcgaccagc cgcgaggccg cgcgcagcac gacggtcgtg
     1621 ccgcgcgtgc gcagcccgtc gatcagctcc ggcgcgttga gcagggcgac gaggatcgcc
     1681 gccgcggcga ccacgaggtc gcgcgtgcgg tcggacgggt gcaggccgcc gagcaccccg
     1741 atcaacacca gcgcgagcac cacccacgtg agccgggcca ggacctgtga acgttggagc
     1801 atttcagggc accttgatcg cgagcggcgc gctggtgacg tcgcacgccg aggtcttcgc
     1861 gagcatcgcg gtgacctcgg ggctggtatt ggcgctctcg cgtttgccgg ggtcgttgat
     1921 ataacgcagc cggacctcgt agcgtccggg gccgtacgac gggccggcga tccacgtcga
     1981 catcgtcttg ctggccccgg gctcgagcgc gaagatctcg ctggcctcga tcgcgttcat
     2041 catgccgcag cccggcgctc gagctgcggc gcgggcttgt cgtcgcgggt gcccgaccag
     2101 gacagcagcg gcgtgcgccg gccgtgctcg ctgccgtcac ccggggtgac gagcgtgcgc
     2161 gccgtggtgc cctcattctt gagggtcagc acatattggg tatcgccgtc ggggccgcgc
     2221 ttgctggcga tcgtcgcccg cagcccgcaa tcgtcgagtt gttcgccggc cggctcgacc
     2281 ggacgcggcg cgacggggat cgacgcggcc ggctcgctgg cctgcgcgac cgcgggtggt
     2341 gccggcgggc tcgccagcgt gaccgcgggg gagggcgggt cgacgggcac cggggcagcg
     2401 cacgcgatcg gcagcaggac ggcgagggcc gtggcgaggg acgcggtcgg tcgcatgcag
     2461 accagcataa cctgctcgcg gacggcagcg cacctggccc catgccctcc cacccgggcc
     2521 atccttcgtc ccccgaggac gccgcccctg gcacgctccg cgatgtcccg gacctcctgc
     2581 ggcaaacggg aagacctgca tggcgaccgg cgcgagccgc ttcgcgcgtg gtggcgcgcg
     2641 atcgcatgcg gcctcaccat gtgtgacgcc gacatccgca gatcgaatgc gagcgggcgg
     2701 ccaccgcgga atatcgatgc atatggaatg tcttgcgggc aattcctcgg acggttgtcg
     2761 ctctcggggt cgcggctcgc gtcgacatga cgcgtgctag ctctgcgggg agcccggagt
     2821 ggcgcaggag gcagatacct gcagactcgc acgagcttgt gaatgcaagc tttgtctctt
     2881 tacccgcaaa cgagcaaacg tcatgaagaa cctcgtgatc ctctcattgc cgctgtgcct
     2941 gctgaccggt ctcgtggtcg gatgcgacgc cgagcccgag tcgaccgcag acctcgcgcc
     3001 ctcgtcgtcc gcggccgcac agggcatgtg gccgctcccg ctgccggccg tcgcggcgca
     3061 gccgaggtct tcgggtcaga agtcggtcaa ggcgaagcac ctgatcgcca aggacgccca
     3121 ggcgacggcc tggacgcgat gggtgatggc gctgccgtac agcaccggcc cgatcaacga
     3181 tccgaccggg gccgcgtgtg cgtcgagcca ggccggcgat ctgtggttcc tcgccgggac
     3241 cgccggtgga tcggccgagc gcacgtgtga cgtgccggcc ggcaagcagc tcgtgtttcc
     3301 gctgctcaac tggttctgcg cgttcttccc cgagatctac cccgacgagc aggccgtcgc
     3361 gcagggggtg tcggagatgg tcgccgcggt cgcctcgttg ccggacggcg tctgcgagct
     3421 gaccctcaag atcgacggcg tcgacgtgct cgccgacctc gacgcggcca acgagctgtt
     3481 cgggttcacc gacgaggcgt tcgacgtcga ggcgaacgac gaccacttcg ccagctcgga
     3541 gggcttcgcg ggcggcacga tcccggcgat caccgcgggc tactacgtgc gcctcaagcc
     3601 gctcgcgccc ggcgaccatg tcctggaatt cggcggagcg ctgtgcaccg atggcaaggt
     3661 ggatttctcg acccacacgt actacaacct gcacgtcggc atgtgatcgg cgtggtcgag
     3721 cgattccccg cactcgagtg cgcttgaaac tgagccggcc cgcgccacgg aaggggcgcg
     3781 gacaggtgtc ccagtaaaac gacccgcaaa gatctgccag cgctgcgcgt atgccggcgt
     3841 tggcagacga gcttgtgagc acggtcgtta tgggatatcc gtggcatctc gatggctggc
     3901 agcgagggcg tacaggcgat gaagagcttc gacggcaagt actcgggcgc cgccttgcag
     3961 gcgttcgtgc a
//
LOCUS       DASWNJ010000720         3191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_649034, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000720 DASWNJ010000000
VERSION     DASWNJ010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649034"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VGB85_11990"
     CDS             complement(<1..238)
                     /locus_tag="VGB85_11990"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY0134800.1"
                     /translation="MPDTTPATSDIAFTPAVKAVQARRGSRTAYARMEARGGFETAIT
                     PDLRELLAVVDTAYLATTNAAGQPYAQHRGGPRGF"
     gene            338..1186
                     /locus_tag="VGB85_11995"
     CDS             338..1186
                     /locus_tag="VGB85_11995"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0134801.1"
                     /translation="MTVFVAVCEAQGFAPAARRLGLSPSVVTRQVAGLEAHLGVRLLQ
                     RTTRSLRLTEAGTRFLERARRIVAEVEEAEQSAQAERASPVGRLVIAAPLIYGRMHVA
                     PAVSRYLERYPGVSAELQLSDRLVGLVEDGIDVAVRIGHLADSGLVARRIGETRRVLV
                     ASPKYLARRGAPQHPRELAEHDLIAFTNLAPGPTWRFVVAGKDLRVAVQPRYATNSGD
                     AAIARARAGGGLCLALAYQVREAIDRGTLVELLPTFAPPALPIHAVFPTSRLLSGKVR
                     AFLELL"
     gene            complement(1223..2263)
                     /locus_tag="VGB85_12000"
     CDS             complement(1223..2263)
                     /locus_tag="VGB85_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134802.1"
                     /translation="MLAHQFMRLAPRSRPGSLAVAALVLGAACSPRPSAVAPPSEPAA
                     PEVQAPCATAEACMTEAVRANEAGELVRAREYFGFACDRGDANGCNESGVMLERDSPL
                     RAARFRSACDLDDAFGCVNLGSVTTDPREAIGASVKGCDLDVKVCDVAARVVIDAEDW
                     TRARALAERGCTDAVDVACGTLGSLLLKGLGGPQDSPRGAVALERGCKAGDTNACENW
                     EFYRSVVSADGAAAAGDLTVPNASLRMGSVSADGYTIRDLACKLDDGGLNVLMVGPML
                     AAAIGSRRAALKKCAPKGGEARVLFNMSGGKTEARAKAVTPAVVACVVKVMTTVEAVS
                     EGLCAVTIDLRQ"
     gene            complement(2393..>3191)
                     /locus_tag="VGB85_12005"
     CDS             complement(2393..>3191)
                     /locus_tag="VGB85_12005"
                     /inference="COORDINATES: protein motif:HMM:NF036933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HEY0134803.1"
                     /translation="LSDKPVALYAGTRFLRLQPTPAPGGEAAHQQNLAVSALGHDYVA
                     APYETRRKDLAPEDVEYRFVGVVDGTELTYDPPLMNAPATLGKGQVVEISTKEAFRVR
                     AQDDQHPFALAQQMDTANLPGGSRPGATAPGFGLALGDEEFVIVLPPAQFLRRYAFFT
                     DPTYATTNLVVTRVRTNNVFEPVTVDCLGELGGWKPVGGDGLFEFTTVDLVRADIGVN
                     GCTNGHHIAESEGAFGVVVWGLDSYSSYAYPAGGSAAVLTDVVVKPQ"
BASE COUNT          414 a         1198 c         1120 g          459 t
ORIGIN      
        1 tgaagccgcg tgggcccccg cggtgctgcg cgtagggctg gcccgccgcg ttcgtcgtcg
       61 ccaggtaggc ggtgtcgacc acggccagga gctcgcgcag gtcgggcgtg atcgccgtct
      121 cgaagccgcc gcgcgcctcc atgcgcgcgt aggcggtccg cgagccgcgg cgggcctgca
      181 ctgccttgac cgcgggcgtg aaggcgatgt cgctggtcgc aggcgtcgtg tcgggcatgg
      241 cgacagcatg cacgcattcc cggcgcggcg taatcttcgc gattgacaag acattgttgc
      301 cggttcagca atagtggact tggaccgcct cgaggccatg accgtgttcg tcgccgtgtg
      361 cgaggcccag ggcttcgcgc ccgcggcgcg caggctcggg ctgtcgccct ccgtcgtcac
      421 ccgccaggtc gccggcctcg aggcgcacct cggcgtgcgc ctgttgcagc ggaccacgcg
      481 atcgctgcgg ttgacggagg ccggtacccg cttcctcgag cgcgcgcggc ggatcgtcgc
      541 cgaggtcgag gaggccgagc agtccgcgca ggccgagcgc gccagcccgg tcggccgcct
      601 cgtgatcgca gccccgctga tctacggccg catgcacgtc gccccggcgg tgtcgcggta
      661 cctcgagcgt taccccggcg tcagcgccga gctccagctc tcggaccgcc tcgtcggcct
      721 cgtcgaggac ggcatcgatg tcgcggtccg catcggccac ctcgccgact ccgggctcgt
      781 cgcacgccgc atcggtgaga cccggcgcgt gctcgtcgcc agcccgaagt acctcgcgcg
      841 ccgtggggcc ccgcagcacc cgcgcgagct cgccgaacac gacctgatcg ccttcacaaa
      901 cctcgcgccc gggccgacct ggcgcttcgt ggtcgccggc aaggacctgc gcgtcgcggt
      961 gcagccgcgc tacgcgacca actcgggcga cgcggcgatc gcccgcgcgc gcgctggcgg
     1021 cggcctgtgc ctcgcgctcg cctaccaggt ccgcgaggcg atcgaccgcg gcacgttggt
     1081 agagctgctg ccgaccttcg ccccgcccgc gctgccgatc cacgcggtgt tcccgacctc
     1141 gcgcctgctc tcgggcaaag tacgcgcatt cctcgagctg ctgtgaggcg ccggccgccg
     1201 cggcccgtcg ccgccgcgcg cctcactggc gcagatcgat cgtcacggcg cagagcccct
     1261 cgctcacggc ctccaccgtc gtcatgacct tgacgacgca ggccacgacg gccggcgtga
     1321 cagccttcgc tctcgcctcc gtcttgccgc cactcatgtt gaacagcacg cgcgcctctc
     1381 cccccttggg ggcgcacttc ttcagcgccg ctctgcgcga gccgatcgcc gccgcgagca
     1441 tcgggccgac catcagcacg ttgagcccgc cgtcgtcgag tttgcaggcg aggtcgcgga
     1501 tcgtgtagcc atcggcggag acggagccca tcctgagcga ggcgttcggg accgtgaggt
     1561 cgcccgccgc ggccgccccg tccgcgctga ccaccgagcg atagaactcc cagttctcgc
     1621 acgcgttcgt gtcgcccgcc ttgcagccgc gctcgagcgc gacggcccct cgcgggctgt
     1681 cctggggccc gccgaggccc ttgaggagca gcgacccgag ggtgccgcaa gcgacgtcga
     1741 cggcgtcggt gcagccgcgc tcggccagcg cgcgcgcgcg cgtccagtcc tcggcgtcga
     1801 tgacgacccg cgccgcgacg tcgcagacct tcacgtcgag gtcgcagcct ttcaccgatg
     1861 caccgatcgc ctcgcgcggg tcggtggtga cgctgccgag gttcacgcac ccgaacgcgt
     1921 cgtccagatc acacgcgctg cggaagcggg ccgctcgcag cggcgagtcg cgctcgagca
     1981 tgacgcccga ctcgttgcag ccgttggcgt cgccgcggtc gcaggcgaag ccgaagtatt
     2041 cgcgcgcgcg cacgagctcc ccggcctcgt tggcgcggac cgcctcggtc atgcacgcct
     2101 ctgcggtcgc acagggggcc tgcacctcgg gcgccgcggg ctcggacggc ggcgcgacgg
     2161 cggacgggcg aggcgagcac gcggcgccca gcacgagggc agcgacggcg aggctgcccg
     2221 gccgggacct gggagcgagc cgcatgaact ggtgcgcgag catgccggaa cctcgcacgc
     2281 ccggcgccgg taatctatcg gtttttgcga cgcgaagccg cacgatcgac gcgccggcgc
     2341 gaagatgcgg atccggtaaa ccctcacctc gctcgccggc tgtcgacgcc gatcactgcg
     2401 gcttgacgac gacgtccgtc agcaccgccg cgctgccgcc tgccgggtat gcgtacgacg
     2461 aatagctgtc gagcccccac accaccacac cgaacgcgcc ctcgctctcg gcgatgtgat
     2521 gaccgttggt gcagccgttg acgccgatgt cggcgcgcac gaggtcgacg gtggtgaact
     2581 cgaacagccc gtcgccgccg accggcttcc acccgccgag ctcgccgagg cagtcgaccg
     2641 tcaccggctc gaacacgttg ttcgtgcgca cgcgcgtgac cacgaggttg gtcgtcgcgt
     2701 aggtcgggtc ggtgaagaac gcgtagcgcc ggaggaactg cgccggcggc agcacgatga
     2761 cgaactcctc gtcgccgagc gccagcccga agcccggcgc cgtcgcccct ggccgcgacc
     2821 cgccgggcag gttcgcggtg tccatctgct gcgccagcgc gaacgggtgc tggtcgtcct
     2881 gcgcgcggac ccgaaacgcc tccttggtcg agatctcgac gacctggccc ttgccgaggg
     2941 tcgcgggcgc gttcatcagc ggtgggtcgt aggtcagctc ggtgccgtcg accacgccga
     3001 cgaagcgata ctcgacatcc tcgggcgcga ggtccttgcg ccgggtctcg tagggcgcgg
     3061 cgacataatc gtggccgagc gccgacacgg cgaggttctg ctggtgcgcc gcctcgccgc
     3121 cgggcgccgg cgtcggttgc agccgcagga agcgggtgcc cgcgtacagc gcgaccggct
     3181 tgtcgctgag c
//
LOCUS       DASWNJ010000721         1902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_398008, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000721 DASWNJ010000000
VERSION     DASWNJ010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398008"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="VGB85_12010"
     CDS             <1..1285
                     /locus_tag="VGB85_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY0134804.1"
                     /translation="DVTQDVVHLGKSRQCDIVLPDESVSRVHAEIRRQGDAYRVLDQQ
                     STNGMFIGGVRVTDGFLKPGDVLALGKVQIRFMPRDARVELLPSTAENFGPVRGRSLG
                     MRKIFGVLERVAGADATILLEGETGTGKDLLARAIHDASPRREGPYVVVDCGGLTAAQ
                     LEIELFGAETGPDVPRERKGAFELAHGGTLLLDDIGELPLDLQPKLTRALENRKVRRG
                     GGSEEQEVDVRVLATANRGLQQQVAASEFREDLYFLIAVVSIQIPALRDRRDDIPMLI
                     DLFSRRLPPGMWRAPGPEAMARLVAYDWPGNVRELRNVVERSAYLSPDGVIDLITAGS
                     LPRSGRDQPVVFDPTLTFREQKERAVELFEETYLNWLLQRSEGNISRAAREADMDRKY
                     LHKLLRRYGIDAKQFTAAALGNRANAEAAGGDSSA"
     gene            1395..>1902
                     /locus_tag="VGB85_12015"
     CDS             1395..>1902
                     /locus_tag="VGB85_12015"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY0134805.1"
                     /translation="MSLSAEPEPGAWVHARGALTLERARVMAVVNLTPDSFSDGGALI
                     DRDREDPNAETVVRVCRELVRQGADILDLGGESTRPGAAPVDAARELRRVLPALRLLR
                     ADPTLADVPISIDTRHAATAAAAMDAGAAIINDVSGLADPEMAAVAARTGAGLVLGHL
                     RGEPATMQE"
BASE COUNT          270 a          695 c          676 g          261 t
ORIGIN      
        1 ggacgtgacc caggacgtcg tgcacctagg aaagtcccgc cagtgtgaca tcgttttgcc
       61 cgatgaatcg gtgtcccgcg tgcacgccga gatccggcgc cagggggatg cgtaccgggt
      121 gctcgaccag cagtcgacca acggcatgtt catcggcggc gtgcgcgtca ccgacggctt
      181 cctcaagccc ggcgacgtcc tggccctggg caaggtccag atccggttca tgccgcgcga
      241 cgcccgggtc gagctgctgc cgagcaccgc cgagaacttc ggtccggtcc gtggtcgcag
      301 cctggggatg cgcaagatct tcggcgtgct cgagcgggtc gccggcgccg acgcgacgat
      361 cctcctcgaa ggcgagaccg gcaccggcaa ggacctgctc gcgcgggcga tccatgacgc
      421 ctcgccgcga cgcgaaggtc cctacgtcgt cgtcgactgc ggcgggctga cggcggccca
      481 gctggagatc gagctgttcg gcgccgagac cgggcccgac gtgccgcgcg agcgcaaggg
      541 ggcgttcgag ctggcccacg gcggcacgct gctgctcgac gacatcggcg agctgcccct
      601 cgatctccag cccaagctga cccgcgcgct cgagaaccgc aaggtccggc gtggtggtgg
      661 cagcgaggag caggaggtcg acgtccgcgt gctcgccacg gccaaccgcg gcctgcagca
      721 gcaggtcgcc gccagcgagt tccgcgagga cctgtacttc ctgatcgcgg tcgtctcgat
      781 ccagatcccc gcgctgcgcg accgccggga cgacatcccg atgttgatcg acctgttctc
      841 gcggcgcctg ccgccgggca tgtggcgcgc cccggggccc gaggcgatgg cgcggctggt
      901 cgcctacgac tggccgggca acgtccgtga gctgcgcaat gtcgtcgagc gcagcgccta
      961 tctctcgccc gacggcgtga tcgacctgat caccgccggc agcctgcctc gcagcgggcg
     1021 cgaccagccg gtggtcttcg acccgacgct gacctttcgc gagcagaagg agcgggcggt
     1081 cgagctgttc gaggagacct acctcaactg gctgctgcag cgctccgagg gcaacatctc
     1141 gcgggcggcc cgcgaggcgg acatggaccg caagtacctg cacaagctcc tgcgccgcta
     1201 cggcatcgac gccaagcagt tcaccgcggc ggccctcggc aaccgcgcga acgccgaggc
     1261 ggccggcggc gattcgagcg cctgaggcgt tcggcccgca cgcgatcacg gcgtcggcgg
     1321 tccgtaaggc cgccgcgtcg cggatgcgcg tgaaattggt ccacgacgcg ccggctcgcg
     1381 ttaccatccc ggcgatgtcg ctgtccgcgg agcccgagcc cggggcgtgg gtccacgccc
     1441 gcggggcgct cacgctcgag cgagcgcgcg tgatggcggt cgtcaatctc acgcccgaca
     1501 gcttctccga cggcggcgcg ctgatcgatc gcgaccgcga ggatccgaac gccgagacgg
     1561 tcgttcgagt gtgccgcgag ctcgtgcgac agggcgccga catcctcgat ctgggcggcg
     1621 agtcgacgcg gccgggcgct gccccggtcg acgcggcccg cgagcttcgc cgcgtgctcc
     1681 cggcgctgcg tctcctgcga gccgacccga cgctcgccga cgtgccgatc agcatcgaca
     1741 ctcgccacgc cgcgaccgcg gccgcggcga tggacgccgg cgccgcgatc atcaacgacg
     1801 tcagcggcct cgccgacccc gagatggccg ccgtcgccgc gcgcaccggc gctggcctcg
     1861 tgctcggtca tcttcgcggc gagccggcga ccatgcagga gg
//
LOCUS       DASWNJ010000722         1952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_261344, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000722 DASWNJ010000000
VERSION     DASWNJ010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261344"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VGB85_12020"
     CDS             <1..268
                     /locus_tag="VGB85_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134806.1"
                     /translation="DAGDNLMQSAGGSVAVSSGERMTMSAGDNLTLVGEKKGLIVIED
                     QLTIRVGEAAIVMKKNGDITITGAKISVLGTGAVKVKGAKLTTN"
     gene            308..784
                     /locus_tag="VGB85_12025"
     CDS             308..784
                     /locus_tag="VGB85_12025"
                     /inference="COORDINATES: protein motif:HMM:NF040117.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6484 domain-containing protein"
                     /protein_id="HEY0134807.1"
                     /translation="MAARKNSAAKVIPMTTPPAPPKARIGHVVGVQDATLLVELAGHG
                     VLRARVAIPLTTAQADAAVAGRTEAIVVFAEGDVERPIVLGLVQSEFSEPVVPTNEAR
                     IDGKRVVLEGKEEVVLKCGEASLTLRANGKVVIRGAYVETRARGTNRIKGGSVQIN"
     gene            complement(796..>1952)
                     /locus_tag="VGB85_12030"
     CDS             complement(796..>1952)
                     /locus_tag="VGB85_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134808.1"
                     /translation="ERALDSPAVDLDGLASGLERRLCAHLDALLVAGPRVAAKLLLPA
                     LADDDPERAFAAALALLAAPEPAHGDAVLRAWSTATPRRRKPLTRALTVAPAAPRRPE
                     LRAALTAADASLRAAAIEVLGFRRALQPRDLDPLRLDPDPAVRHALVRAAGRDPTLDL
                     RSLVRDALHAQQPEHLAAALASAAILGLPELPAARQQARARDDEAGALALLLTAFTDP
                     AALLAELDRPRAVFALGFTGSFAAAEACVRCIRDGKHPALAFEAFTALTSADLRHAAT
                     PDDDALPELADEDLTADIASGPEDDLPLPDPDLVQAWWRANADRLPRPRLPTLADDRR
                     GLLLALRHAPMRRRHALALRLAIVTAGAAQLETRAPALRQRTALQRLAGT"
BASE COUNT          278 a          559 c          890 g          225 t
ORIGIN      
        1 cgacgccggt gacaacctga tgcagtccgc gggcggcagc gtggcggtgt cgtcgggcga
       61 gcgcatgacg atgtcggccg gcgacaacct gacgctggtc ggcgagaaga agggcctgat
      121 cgtgatcgag gaccagctga cgatccgcgt cggcgaggcg gcgatcgtga tgaagaagaa
      181 cggcgacatc acgatcacgg gcgcgaagat ctcggtgctg gggaccggcg ccgtcaaggt
      241 caagggcgcc aagctgacga ccaactgagc gacgagagag acacacacag aggactggag
      301 gagacttatg gctgcccgga agaacagcgc tgcgaaggtg atcccgatga cgacgccgcc
      361 ggctccgccg aaggcgcgga tcggtcatgt ggtgggagtg caggacgcga ccttgctggt
      421 cgagctcgcc gggcacgggg tgctgcgcgc gcgcgtggcg atcccgctga cgacggcgca
      481 ggcggacgcg gcggtggccg ggcggaccga ggcgatcgtg gtgttcgcgg agggggacgt
      541 cgagcggccg atcgtgctcg ggctggtgca gtcggagttc tccgagccgg tggtgcccac
      601 gaacgaggcg cgcatcgacg gcaagcgggt cgtgctcgag ggcaaggaag aggtggtgct
      661 caagtgcggc gaggcgtcgc tgacattgcg cgccaacggc aaggtcgtga tccgcggggc
      721 ttacgtcgag acgcgcgcgc gcgggacgaa ccgcatcaag ggcgggtcgg tgcagatcaa
      781 ctgacgatca ctcgatcagg tgccagcgag ccgctgcagg gcggtgcgct ggcgcagcgc
      841 gggggcgcgg gtctcgagct gcgctgcgcc ggcggtgacg atcgcgaggc gcagcgcgag
      901 ggcgtgacgg cggcgcatcg gggcgtggcg cagggcgagc agcaggccgc ggcggtcgtc
      961 ggcgagggtc ggcaggcgcg ggcgcgggag ccggtcggcg ttcgctcgcc accacgcttg
     1021 cacgaggtcg gggtcgggga gagggagatc gtcctcgggg ccgctggcga tgtcggcggt
     1081 gaggtcctcg tcggcgagct cgggcagggc gtcgtcgtcg ggcgtggcgg cgtggcgcag
     1141 gtcggcggag gtcagcgcgg tgaaggcctc gaaggcgagg gcggggtgct tgccgtcgcg
     1201 gatgcaacga acgcaggcct cggcggcggc gaagctgccg gtgaagccga gggcgaacac
     1261 ggcgcgcggg cgatcgagct cggcgagcag cgcggccgga tcggtgaagg cggtcagcag
     1321 gagggcgagc gcgccggcct cgtcgtcgcg ggcacgcgcc tgctggcgtg cggccggcag
     1381 ctcgggcagg ccgaggatcg cggcgctggc gagcgcggcg gcgaggtgct ccggttgttg
     1441 agcatgcaac gcgtcgcgga cgagagatcg caggtcgagg gtcgggtcgc ggccggcggc
     1501 gcgcacgagg gcgtggcgga cggcggggtc ggggtcgagg cgcagggggt cgaggtcgcg
     1561 tggttgcagg gcgcggcgga agccgaggac ctcgatcgcg gcggcgcgca gcgaggcgtc
     1621 ggcagcggtc agggcggcgc gcagctcggg gcggcgaggc gcggcggggg cgacggtgag
     1681 ggcgcgggtc agtggtttac ggcggcgggg cgtggcggtc gaccaggcgc gtagtactgc
     1741 gtcgccgtgg gccggttcgg gcgcggcgag cagggcgagg gcggcggcga aggcccgctc
     1801 ggggtcgtcg tcggcgaggg cggggagcag gagcttggcg gcgacgcgcg ggccggcgac
     1861 cagcaacgcg tcgaggtggg cgcacaggcg gcgctcgagg ccgctggcga ggccgtcgag
     1921 gtcgacggcg gggctgtcga gggcgcgctc cc
//
LOCUS       DASWNJ010000723        11427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_118954, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000723 DASWNJ010000000
VERSION     DASWNJ010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 11427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11427
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118954"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1293)
                     /locus_tag="VGB85_12035"
     CDS             complement(<1..1293)
                     /locus_tag="VGB85_12035"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0134809.1"
                     /translation="MQAQPRAPGHSAEEAGLDSEGPTREDRAAGGPRFAAQAATDDVR
                     KASLLTGTGARIGRFHVLRLIGEGGMGMVFAAYDEELDRKVAIKLLRNLPGEGSIGRT
                     RLLREAQALARLSHPNVVTVYEVGEFEQQVFIAMEFVLGDTLYKWLRDGARPWREVVR
                     VFIEAGRGLAVAHAAGILHRDFKPANVLLGTDGRVRVLDFGLALAQGRRDGDAELVGA
                     HSGSSSSMRQEALTEAGAVLGTPAYMAPERYIAGRTVDARVDQFSFCVALHEALYGAR
                     PFPGREYAELRKTILRGTIPEAPRGSKVPKWLRRVVLRGLALDPANRFPDMEALLVAL
                     QRDPDRTRRLVFAGLGVGAVLIAGGYGLAQATGGPALCPDARERLAWDSRRAEVERAF
                     AGTQVSYAADTWTRISGELDTYADTWTAGHSDACESHAR"
     gene            complement(1381..2148)
                     /locus_tag="VGB85_12040"
     CDS             complement(1381..2148)
                     /locus_tag="VGB85_12040"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0134810.1"
                     /translation="MSRVSGPARAAMMAIAGMLVMACAGRSVSPRVAGGTEVVRIERA
                     YINSYLLIADRVVVVDTGVPGSAKRVLRALRRRGLSPQDVALIVLTHAHADHAGAAAE
                     LRARTGAAIVAGAGDEAMTTRGHNDAMKPSARLGRAVRPFIGKDYPRFTPDLLLTQPL
                     DLHPFGVPGRVLPMPGHTPGSLAVVLDDGQAVAGDLVRGRLLAPRRPVLHFFHDDPTR
                     AHGQLAALLDEHGVLRLHPGHGHALTAAALRAFLAGR"
     gene            2293..2514
                     /locus_tag="VGB85_12045"
     CDS             2293..2514
                     /locus_tag="VGB85_12045"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEY0134811.1"
                     /translation="MNTSSATVAKVIEISSQSPTSFQDAIDAGVARAAQTLRNVEGVW
                     IKEQSLSVADGKVKAYRVVMKVTFVFDGT"
     gene            2518..2712
                     /locus_tag="VGB85_12050"
     CDS             2518..2712
                     /locus_tag="VGB85_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134812.1"
                     /translation="MTSATEPSTSRVRAIALVALGALLVAFAVLYFRARSSEGGTQRD
                     DSPAAEAGVGTPNGGGGNQR"
     gene            complement(2726..4378)
                     /locus_tag="VGB85_12055"
     CDS             complement(2726..4378)
                     /locus_tag="VGB85_12055"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY0134813.1"
                     /translation="MRRFAALYAALDETNKTGDKVAAMQRYFAEAPPEDAAWAVFFLT
                     GRKPRQVISTRNLCAWAIAAAQIPDWMFEQSYEAVGDLAETVALLLPQAPADSKRPES
                     MPLHRWVETRLLTLPQLPEEAQRQMMLAAGEEFNPTERLVWNKLITGAFRVGVSQTLV
                     IRALSEAGGVPVATVAHRLMGTWTPDAAMFRGLMAAESGDAVLSRPYPFMLAHALEDP
                     PASLGAPETWQAEWKWDGIRAQIVRRGDEVFVWSRGEELIGERFPEIALAAKKLPEGT
                     VLDGEVLPWKNGEVLPFAELQRRIGRKTLTRKILEEVPAIFLAFDVLEHEGKDIRETA
                     MQARRALLEMIVETLGSSTITTSHVVPVASWEEAAKLREQSRSRRVEGFMLKRLDSRY
                     GLGRVRGDWWKWKIDPYVVDAVLIYAQGGQGRRASLFTDYTFAVWRNGELVPFCKAYS
                     GLTDDEIRQVDAFVRAHTREQFGPVRVVDPELVFEIGFEGIARSTRHKSGVAVRFPRM
                     LRWRQDKRADEADAIERLIALLPGGDKDADTMEAGAGPASGD"
     gene            complement(4375..5442)
                     /locus_tag="VGB85_12060"
     CDS             complement(4375..5442)
                     /locus_tag="VGB85_12060"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HEY0134814.1"
                     /translation="MPAPLVELTEDGLLCRAGGFYVDPWRPVERAIITHAHGDHAQAD
                     SEHYLAATPGLGVLRLRVGASANIHGLAYGEAIKLGEVTVSLHPAGHILGSAQVRIER
                     AGEVWVVSGDYKLGLDPTCTPFEPVRCHTFISESTFGLPVYRWAPQSEIFEDINNWWA
                     GNKAAGKASLLLCYALGKAQRILAGIDGSIGPVYLHGAVERVTQAYRAAGVLMVPTKL
                     VGPAKRGDDWKGSLILAPPSALHTPWGRRFRPLVSGFASGWMRVRGARRRRAVDRGFV
                     LSDHADWPGLLAAISATGASRVLVTHGHAAPLTRFLQEQGLEAEVLATQFVGEGEEAS
                     DAGAENKEPARETEASEEVAS"
     gene            complement(5497..6582)
                     /locus_tag="VGB85_12065"
     CDS             complement(5497..6582)
                     /locus_tag="VGB85_12065"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEY0134815.1"
                     /translation="MLLSRQRTTVLAAVERLVGMQAQLARPPHVGLWSRLHGFAREDL
                     NGALQRRELVRATLMRCTLHLMSARDYVDLRAVIQPVLDRAMKSVLRERTDGLGLPTL
                     MAAARRHLQEKPRTFEVLRDLLVEGDPEGDARAMGYAVRCLLPLVQVPTDTPWGFPAA
                     ADFALADEWLGQPIPTAEASPLPLISRYLAAFGPATVGDMQTWSGLQGLRESFEALRP
                     TLVTFRDERGRELFDLPDAPRPPEDVEAPVRFIPDFDNLILGHDDRTRLIADEHRAAI
                     FLPNLRILPTFLVDGRVAGTWKVEAKKASATLVIEPFAAISRPARAALTEEGDGLLRF
                     LEPEAASFAVRVAGASIAKPRRVAKPK"
     gene            6758..7333
                     /locus_tag="VGB85_12070"
     CDS             6758..7333
                     /locus_tag="VGB85_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134816.1"
                     /translation="MPPPPPPLYTGTSISVFELDGLIGIRPRPGHAFTSPAGTSVLYL
                     PFATMVSLTVDPIDGDSAALEFHGDFRRTAAVSRTRLAPIAAGDGEPVAEFLRARHGL
                     PRTPNVRTPAELRALEDAEYVTVVATYRPGHFETANFEGLMLEHEDRKTLAPGDRVEA
                     TGFYYPGHSGDYPRRVGYGGPRLHALVMRRL"
     gene            complement(7352..8809)
                     /locus_tag="VGB85_12075"
     CDS             complement(7352..8809)
                     /locus_tag="VGB85_12075"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0134817.1"
                     /translation="MAWYKWAAARSSVVMMVVAVGCSSGAREDDTGGSTGATMPTMPT
                     SSTGATSTTDPATSTTTTGETPTTGTPDPSTTQTCVDGQEGCPCLPLEACAGELQCLE
                     GLCVAVSGSTSTTETGSTTGGDESSTGVVMAVCGDGEKAAGELCFAMATPLGMGAGPV
                     AVAVADFNKDGDLDIVSANNTSNNLTVRTGKGDGGFNSLSALATGMGPYALATPDINN
                     DTAPDIVAANYGSSTLTIYLNNGAGMMVGQPAIGAGTNPRSLATANVNGDEYDDIFVA
                     NYANSTLGVYIGDGIGYPNPTTVAAGVNPTWVALADVNGDGKLDLASANYGASTIGVS
                     LGNGLGNFGPATPSPVGGMGYGLALADFNSDMKPDAVVTVPAANSIYLMQGNGLGGFG
                     GAIPYPSGTEPLGIMVEDMDSDGHADVIVANPGANMVSVLRGDGNGGLGLPESFMVGI
                     MPYGVGIGDFNGDGVLDMVTPNNGDSTVSVLLSNP"
     gene            complement(8987..10321)
                     /locus_tag="VGB85_12080"
     CDS             complement(8987..10321)
                     /locus_tag="VGB85_12080"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HEY0134818.1"
                     /translation="MHDASHVIFGLDPLWVSTALLLMTYAVIMSEKVNRAVVALLGAG
                     LMICLGVINQEAAIRGVDFNTIALLTGMMIIVNITRKSGVFQYVAFWAARRAEGVPWK
                     LMIMLSVVTAVLSALLDNVTTVLLVTPVTLMVARELEVDPYPLLFAEVFASNIGGTST
                     LIGDPPNILIGSATGLSFNDFVVNLAPIVCVIMGLQLLATHLLWGRKMQTSEEARRRI
                     LAFRPADAITDPALLRRSLTVLALVIGGFVSARATGIEPGTVAMFGAASLMYLFIKGH
                     HAEEQSSHVHEVFGEVEWITIFFFIGLFMVVAGVEHAGLLGNLAELLLSATDGDRTVT
                     ALVILWSSAILSAIIDNIPFVATMIPMIKNMAPTMGGEQALMPLWWALSLGACLGGNG
                     TLVGASANLTVAGLAERGGVSFKFMTYTKVAFPLMLGSIVVAHIYLYFAFLR"
     gene            10557..>11427
                     /locus_tag="VGB85_12085"
     CDS             10557..>11427
                     /locus_tag="VGB85_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134819.1"
                     /translation="MDGDPHGLWILGISVAALLLGPAIHLLARRAPPTMAALDNFVLV
                     AVVGLGALEILPGALHLAGLPAFIVLLLGALGPALADGPLHRLSRGTHGAALALGVVG
                     MAIHSFTDGLALASAHAAGNHSHALEAAVIAHQLPVAVAVWWLIVPIRRLGWIGATVA
                     MVGLGLATILGFELADASLGALTPAWLGLLQALFAGFLLHVIAHRSHPVGEDTGASRT
                     IHVAAALGGLVGLGLLALIGVDGMDAGHAGHLDSGHAGEAHGVLPDIFHLARAAAPPI
                     VAALALALLIGRPL"
BASE COUNT         1656 a         4396 c         3704 g         1671 t
ORIGIN      
        1 gcgcgcgtgg ctctcacagg cgtcgctatg ccccgcggtc catgtatcgg catatgtatc
       61 gagctcgccg gagatcctcg tccaggtgtc ggccgcatag ctgacctgcg tgccggcgaa
      121 ggcgcgctcg acctccgcac gacgcgagtc ccaggcgagc cgctcgcgcg cgtcggggca
      181 cagcgccggt cctccggtcg cctgcgccag cccgtagccg ccggcgatca gcaccgcgcc
      241 gacgccgagc cccgcgaaca ccagccgccg cgtgcggtcg gggtcgcgtt gcagggcgac
      301 cagcagcgcc tccatgtcgg ggaagcggtt cgccgggtcg agcgcgagcc cccgcagcac
      361 caccctgcgt agccacttcg gcaccttcga cccgcgcggc gcctcgggga tcgtgccgcg
      421 caggatcgtc tttcgcagct cggcgtattc gcggccgggg aaagggcgcg ccccgtacag
      481 cgcctcgtgc agcgcgacgc agaagctgaa ctgatcgacc cgcgcgtcga ccgtgcgccc
      541 ggcgatatac cgctccgggg ccatgtacgc cggcgtgccg agcaccgccc ccgcctccgt
      601 caacgcctcc tgacgcatgc tcgacgacga ccccgagtgc gcgcccacca gctccgcgtc
      661 gccgtcgcga cggccctgcg ccagcgcgag cccgaagtcg agcacacgca cccggccgtc
      721 ggtgccgagc agcacgttgg ccggcttgaa gtcgcggtgc aagatgcccg ccgcgtgcgc
      781 gaccgccagc ccgcgcccgg cctcgatgaa cacgcggacc acctcgcgcc acggccgggc
      841 cccgtcccgc agccatttat aaagcgtgtc accgagcacg aactccatgg cgatgaacac
      901 ctgctgctcg aactcaccga cctcgtagac ggtgaccacg ttcgggtgcg acagccgggc
      961 cagcgcctgg gcctcgcgca gcagtcgagt gcgtccgatg ctgccctcgc ccgggaggtt
     1021 acgcagcagc ttgatcgcca ccttgcggtc gagctcctcg tcgtaggcgg cgaacaccat
     1081 gcccatgccg ccctcgccga tcaggcgcag cacatgaaac cggccgatcc ttgcgccggt
     1141 cccggtgagc aggctggcct ttcggacatc atccgtcgcg gcctgcgcgg cgaacctcgg
     1201 tccgcccgcg gccctgtcct cgcgcgtcgg cccctcggag tcgaggcccg cctcctccgc
     1261 cgaatggcca ggggctcgcg gctgagcttg cacgcctcat aggagccggt gccagcgccc
     1321 cacgcaagcc cgctgtgcac gcatgaggtc gcacgcacgc gcacgatcgc gcggactctt
     1381 tcagcggccc gcgaggaacg cccgcagcgc ggcggccgtc agcgcgtgac catgcccggg
     1441 atgcaggcgc agcaccccgt gctcgtcgag cagcgccgcg agctgcccgt gcgcccgcgt
     1501 cgggtcgtcg tgaaagaagt gcagcaccgg ccggcgcggc gccagcaagc gcccgcgcac
     1561 caggtcgccc gcgaccgcct gcccgtcgtc gagcaccacc gccagcgagc cgggcgtgtg
     1621 gcccggcatc ggcagcacgc gtccgggcac gccgaagggg tgcaggtcga gcggctgcgt
     1681 cagcagcagg tccggtgtga agcgcgggta gtccttgccg atgaacggcc ggaccgcgcg
     1741 accgagccgc gcactcggct tcatcgcgtc attgtgcccg cgcgtcgtca tcgcctcgtc
     1801 gcccgcgccc gcgacgatcg ccgcgccggt gcgcgcgcgc agctccgccg cagcgccagc
     1861 gtggtccgcg tgagcatgcg tcagcacgat cagcgcgacg tcttgcggcg agagcccgcg
     1921 ccgccgcagc gctcgcagca cccgcttcgc gctgcccggc acgcccgtgt cgaccacgac
     1981 cacgcggtcg gcgatcagca ggtagctgtt gatgtacgcc cgctcgatgc ggaccacctc
     2041 cgtaccgccc gcgacccgcg gtgacacgct gcgcccggcg cacgccatca ccagcattcc
     2101 tgcgatcgcc atcattgctg ctctcgcggg gcccgacaca cgcgacatct gccgagcgta
     2161 gcgtatccgg ccgctggcgt cttctgcgca ggatgccccg tgaaaccgtg ccggcgtcga
     2221 caaacgaccg ggatacgtcc gtaccggcgc atgagtgctt gccctgtcac gcaggcgagg
     2281 catgtcacgc gcatgaacac ctcatccgcc accgtcgcca aggtcatcga gatcagctcg
     2341 cagtctccca ccagctttca ggacgcgatc gacgccggcg tcgcccgggc cgcgcagacc
     2401 ctgcgtaacg tcgagggcgt gtggatcaag gagcagtcgc tctcggtcgc cgacggcaag
     2461 gtcaaggcct atcgcgtggt catgaaggtc accttcgtgt tcgacgggac ctgatcgatg
     2521 acctccgcca ccgaaccctc gacctcccgc gtccgcgcga tcgccctcgt ggcgctcggc
     2581 gccctgctcg tcgccttcgc cgtgctctac ttccgggccc ggtcgagcga gggcggcacc
     2641 cagcgtgacg actcacccgc cgcggaagcc ggcgtcggca ccccgaacgg cggcggcgga
     2701 aatcagcgct gatccacgga tcgcttcaat ccccggaggc cggccccgcg ccggcctcca
     2761 tcgtatctgc gtccttgtcc ccgccgggca gcagcgcgat caatcgctcg atcgcgtcgg
     2821 cctcgtcggc gcgcttgtcc tgccgccagc gcagcatacg agggaatcgg accgcgacgc
     2881 cggacttgtg gcgggtcgag cgggcgatgc cctcgaagcc gatctcgaac accagctcgg
     2941 ggtcgacgac gcggaccggc ccgaattgct cgcgggtgtg cgcgcggacg aacgcgtcca
     3001 cttgccggat ctcgtcgtcg gtcagccctg aataggcctt acaaaacggc accagctcgc
     3061 cgtttcgcca caccgcgaat gtatagtcgg taaacaggct cgcgcgccgc ccctggccgc
     3121 cctgcgcgta gatcagcacc gcgtcgacga cgtacgggtc gatcttccac ttccaccagt
     3181 cgccgcgcac gcgcccgagg ccgtagcgcg agtcgaggcg cttgagcatg aagccctcga
     3241 cgcggcggct gcggctctgc tcgcgcagct tcgccgcctc ctcccacgac gcgaccggca
     3301 ccacgtgcga ggtcgtgatc gtcgacgacc cgagcgtctc gacgatcatt tccaaaagcg
     3361 cccgccgggc ctgcatcgct gtctcgcgga tatccttgcc ttcgtgctcg agcacgtcga
     3421 acgcgaggaa gatcgccggt acttcctcga gaatcttccg cgtcagggtc ttgcgcccga
     3481 tccttcgctg cagctcggcg aatggcagca cctcgccgtt tttccagggc aacacctcgc
     3541 cgtcgagcac ggtgccctcg ggtagcttct tcgccgcgag cgcgatctcc ggaaaacgtt
     3601 cgccgatcag ctcctcgccg cgcgaccaca cgaacacctc gtccccgcgg cggacgatct
     3661 gcgcgcggat gccgtcccac ttccactcgg cctgccacgt ctccggcgcg ccgaggctcg
     3721 cgggtgggtc ctcgagcgcg tgcgcgagca tgaaggggta cggccgcgag aggaccgcgt
     3781 cgcccgactc ggcggccatc agcccgcgga acatcgccgc gtccggcgtc cacgtcccca
     3841 tcagtctatg ggcgaccgtc gccaccggca cgccgccggc ctccgacagc gcgcggatca
     3901 ccagtgtctg cgacacgccg acgcgaaacg cccccgtgat cagcttgttc cacaccaggc
     3961 gctcggtcgg attgaactcc tcccccgccg ccagcatcat ctggcgctgc gcctcctccg
     4021 gcaattgtgg caacgtgagc agacgggtct cgacccagcg gtgcagcggc atcgactcgg
     4081 gccgtttgct atccgcgggc gcctgtggca gcagcagcgc gaccgtctcc gcgaggtcac
     4141 cgaccgcctc gtagctctgc tcgaacatcc agtcggggat ctgcgccgcc gcgatcgccc
     4201 acgcgcacag gttccgcgtc gagatcacct gccgcggctt tctccccgtc aaaaagaaca
     4261 ccgcccacgc cgcgtcctcc ggcggcgcct cggcgaagta gcgctgcatc gccgcgacct
     4321 tgtcgccggt cttgttggtc tcgtcgagcg cggcgtacaa agcagcaaat cgcctcatga
     4381 cgcgacctcc tcgctcgcct cggtctcgcg cgcgggctcc ttgttctccg cgccggcgtc
     4441 gctggcctcc tcgccctcac cgacgaattg cgtcgccagc acctccgcct cgagcccctg
     4501 ctcctgcaaa aatcgggtca gcggcgccgc gtgtccgtgg gtcacgagca cgcggctcgc
     4561 cccggtcgcc gagatcgccg cgagcagccc cggccagtcg gcgtggtcgg agagcacgaa
     4621 gccgcggtcg accgcgcgcc ggcgcctggc cccgcgcacg cgcatccagc ccgaggcgaa
     4681 gccgctgacc agcggccgga agcgccgccc ccacggtgta tgcagcgccg agggcggcgc
     4741 gaggatcagc gagcctttcc agtcgtcgcc gcgcttcgcc ggcccgacga gcttcgtcgg
     4801 caccatcaat acgcccgcgg cgcgatacgc ctgcgtgacc cgctcgaccg ccccgtgcag
     4861 gtacaccggc ccgatgctgc cgtcgatccc cgcgaggatc cgctgggcct tgccgagcgc
     4921 gtaacacagc aacagcgacg ccttcccggc cgccttgtta ccggcccacc aattattgat
     4981 atcctcgaaa atttcggact gcggtgccca gcgatagacc ggcaggccga aggtggattc
     5041 acttataaat gtgtggcagc ggaccggctc gaagggcgtg caggtcgggt cgagcccgag
     5101 cttgtaatct cccgacacga cccacacctc tcctgcccgc tcgatccgca cctgcgccga
     5161 gccgaggatg tgcccggccg ggtgcagcga caccgtcacc tcaccgagct tgatcgcctc
     5221 gccgtacgcg agcccgtgaa tgttcgccga ggccccgacg cgcaggcgca gcaccccgag
     5281 ccccggcgtc gccgcgaggt aatgctcgct gtccgcctgc gcgtggtcgc cgtgcgcgtg
     5341 ggtgatgatc gcccgctcga ccggccgcca ggggtcgaca taaaacccgc ccgcccggca
     5401 gagcaggccg tcctcggtga gctcgacgag cggcgcgggc atgggcagcc cttccatagc
     5461 acccgatctc gcgctcgcca gcgcgcctcc accgtatcac ttcggcttgg ccacgcgccg
     5521 cggcttggcg atcgatgcac ctgcaacgcg gaccgcgaag ctcgcggcct cgggctcgag
     5581 gaaccgcagc aagccatcac cctcctcggt gagggccgcg cgcgccggcc gcgagatcgc
     5641 ggcgaatggt tcgatgacga gggtcgcgga cgccttcttg gcctcgacct tccacgtccc
     5701 ggcgacgcgc ccgtcgacga ggaaggtcgg gaggatgcgc aggttcggca ggaagatcgc
     5761 cgcgcggtgc tcgtcggcga tcagccgcgt gcggtcgtcg tggccgagga tcaggttgtc
     5821 gaagtcgggg atgaagcgca ccggcgcctc gacatcctcc ggggggcgcg gcgcgtcggg
     5881 caggtcgaac agctcgcgcc cacgctcgtc gcggaacgtc accagcgtcg gccgcaacgc
     5941 ttcgaagctc tcgcgcagcc cctgcaggcc cgaccaggtc tgcatgtcgc cgacggtcgc
     6001 cggcccgaac gcggccaggt aacgcgagat cagcggcagc gggctcgcct ccgcggtcgg
     6061 gatcggctgg ccgagccact cgtccgcgag cgcgaagtct gccgccgccg ggaagcccca
     6121 cggcgtgtcg gtcggcacct gcaccagcgg taacaaacag cgcaccgcgt agcccatcgc
     6181 ccgcgcgtcg ccctcggggt cgccctcgac cagcaggtcg cgcagcacct cgaaggtgcg
     6241 cggcttctcc tgcaggtgcc tgcgcgcggc cgccatcagg gtcggcagcc cgagcccgtc
     6301 ggtccgctcg cgcagcaccg acttcatcgc ccggtcgagc accggctgga tcaccgcgcg
     6361 tagatcgacg tagtcgcgcg cgctcatcag gtgcaaggtg cagcgcatca gcgtcgcgcg
     6421 caccagctcg cgccgctgca gcgcgccgtt gaggtcctcg cgggcaaacc catgcagccg
     6481 cgaccacagc ccgacgtggg gcgggcgcgc cagctgggcc tgcatgccga cgaggcgctc
     6541 gaccgccgcg agcacggtcg tgcgctggcg actcagcaac atctgtcgcg ccagcgtcgc
     6601 gcgcccgagc gcacgcgggc cgagggtcgg tgttgcccct gcgctcgccg tcttcgctgc
     6661 tgctgccttc ggtgccttcg ctgctgccac catgacagag taccccagag tacccctgac
     6721 gacggcggcc gtgcgctgca actgatattc tcggcggatg ccgccgccgc caccaccgct
     6781 gtacaccggc accagcatct ccgtgttcga gctcgacggc ctgataggga tccgcccgcg
     6841 ccccggccac gcgttcacct cgcccgcggg caccagcgtc ctttatctcc cgttcgccac
     6901 catggtctcg ctcacggtcg acccgatcga cggcgacagc gcggcgctcg agttccacgg
     6961 tgacttccgg cgcaccgccg ccgtgagccg cacccggctc gccccgatcg ccgccgggga
     7021 cggcgagccg gtcgccgaat tcctgcgcgc ccgccacggc ctgccgcgca ctccgaacgt
     7081 ccgcaccccg gccgagctgc gagccctcga ggacgctgaa tacgtcaccg tcgtcgccac
     7141 ctacaggccc ggccacttcg agaccgccaa cttcgagggc ctcatgctcg agcacgagga
     7201 ccgcaagacc ctcgcccccg gcgaccgcgt cgaggcgacc ggcttttatt atcccggcca
     7261 ctccggcgac tacccgcgcc gcgtcggtta cggcgggccg cgcctgcacg cgctggtcat
     7321 gcgccgcctc tgagccccgc ggcgccgacc ctcaggggtt cgacagcagc acgctgaccg
     7381 tgctgtcgcc attgttcggc gtgaccatgt cgagcacgcc gtcgccgttg aagtcgccga
     7441 tcccgacccc gtacggcatt atgccgacca tgaacgactc gggcaggccg aggccgccgt
     7501 tgccgtcgcc gcggagcacg ctgaccatgt tggccccggg gttggcgacg atgacgtcgg
     7561 cgtggccatc gctgtccatg tcctcgacca tgatgcccag cggctcggtc ccgctcgggt
     7621 aggggatcgc gccgccgaag ccgccgaggc cgttgccctg catgaggtag atcgagttcg
     7681 cggccggcac cgtcacgacc gcgtccggct tcatgtcgct gttgaagtcc gccagcgcca
     7741 ggccgtagcc catcccgccg accggcgacg gcgtcgcggg gccgaagttg ccgaggccgt
     7801 tgccgagcga cacgccgatc gtgctcgccc cgtagttcgc gctggcgagg tcgagcttgc
     7861 cgtcgccgtt gacgtccgcg agcgcgaccc acgtcgggtt gacgcctgcc gcgaccgtcg
     7921 tcgggttcgg gtagccgatg ccgtcgccga tgtacacgcc gagcgtcgag ttcgcgtagt
     7981 tggcgacgaa gatgtcgtcg tactcgtcgc cgttgacgtt ggccgtggcg agcgagcgcg
     8041 ggttcgtccc cgcgccgatc gccggctggc cgaccatcat gccggcgccg ttgttgaggt
     8101 agatcgtcag cgtcgagctg ccgtagttgg ccgcgacgat gtccggcgcg gtgtcgttgt
     8161 tgatgtccgg cgtggcgagc gcgtacgggc ccatgccggt cgccagggcc gacagcgaat
     8221 tgaagccgcc gtcgcccttg cccgtgcgca ccgtcaggtt gttcgaggtg ttgttcgccg
     8281 acacgatgtc gaggtcgccg tccttgttga agtccgccac cgccaccgcc accggccccg
     8341 cgcccatccc cagcggcgtc gccatcgcaa agcacagctc gcccgccgcc ttctcgccgt
     8401 cgccgcagac cgccatcacg acgcccgtgc tcgactcgtc gccgccggtc gtgctgccgg
     8461 tctcggtcgt cgaggtcgag cccgacaccg ccacgcacag cccctccagg cactgcagct
     8521 cgcccgcgca cgcctcgagc ggcagacacg gacagccctc ctggccgtcg acgcaggtct
     8581 gcgtcgtcga cgggtcgggc gtcccggtcg tcggcgtctc gccggtggtg gtcgtgctcg
     8641 tcgcggggtc cgtcgtcgac gtcgcgccgg tcgagctcgt cggcatcgtc ggcatcgtcg
     8701 cgcccgtcga gccgccggtg tcgtcctcac gcgcaccgct cgaacacccg accgccacca
     8761 ccatcatcac cacggaagaa cgcgccgctg cccacttgta ccaagccatg gccgcaagct
     8821 tggctgcccg tccgggggcc gtcaaccggc cgcgcgccgc gcaatctcgg cccagcgaag
     8881 gcgagaaggc agcgaaggcg agaaggcagc gaaggcgaga agggaatggc cggcgcgagg
     8941 ggccccacgg tgtgaagccc ctcgcacgcc gggggcgcgc ctgcgctcag cgcaggaacg
     9001 cgaagtacag gtagatgtgg gcgacgacga tgctgccgag catcagcggg aacgcgacct
     9061 tggtgtaggt catgaacttg aacgacacgc cgccgcgctc ggcgaggccg gcgacggtga
     9121 ggttggcgct cgcgccgacc agggtcccgt taccgccgag acatgccccg agggacaggg
     9181 cccaccacag cggcatcagg gcctgctcgc cgcccatcgt cggcgccatg ttcttgatca
     9241 tcgggatcat ggtcgccacg aacgggatgt tgtcgatgat cgccgacagg atcgccgagg
     9301 accacaggat caccagcgcc gtcaccgtgc ggtcgccgtc ggtcgcgctg agcagcagct
     9361 ccgccaggtt gccgagcagc ccggcgtgct cgaccccggc gacgaccatg aacaggccga
     9421 tgaagaagaa gatcgtgatc cactcgacct cgccgaacac ctcgtgcacg tgcgacgact
     9481 gctcctccgc gtggtgcccc ttgatgaaca ggtacatcag cgaggccgcc ccgaacatcg
     9541 ccaccgtgcc cggctcgatg ccggtcgccc gcgcgctgac gaagccgccg atcaccagcg
     9601 ccagcaccgt caaggagcgc cgcagcagcg ccgggtcggt gatcgcgtcc gccggacgaa
     9661 aggcgaggat ccgccggcgc gcctcctcgc tggtttgcat cttccggccc cacagcaggt
     9721 gggtcgccag cagctgcaga cccatgatga cgcacacgat cggcgccagg ttcaccacga
     9781 agtcgttgaa gctcagcccc gtcgccgagc cgatcaggat gttcggcggg tcaccgatca
     9841 gcgtcgaggt gccgccgatg ttcgaggcga acacctcggc gaacagcagc gggtacgggt
     9901 cgacctcgag ctcgcgcgcg accatcagcg tcaccggcgt caccagcagc accgtcgtca
     9961 cgttgtcgag cagcgccgac agcaccgccg tcaccacgga cagcatgatc atcagcttcc
    10021 acggcacgcc ctcggcccgc cgcgcggccc agaacgccac gtactggaac accccgctct
    10081 tgcgcgtgat gttgacgatg atcatcatcc ccgtcaaaag cgcgatcgtg ttgaagtcga
    10141 ccccgcggat cgccgcctct tgattgatga cgccgaggca gatcatcaac ccggcgccga
    10201 gcaacgcgac gaccgcccgg ttcaccttct cggacatgat gaccgcgtag gtcatcaaca
    10261 acagggccgt cgagacccac agtggatcga ggccgaagat cacatgagat gcgtcatgca
    10321 tgagctgtct ctgagggcag gatgtaaagg cgagatcgcg gcctgtcctc ggcgcggggc
    10381 ttagcgcagc ctctccaggt tgccaagtgc atgtatgtga gttttgaacg cggttccgtg
    10441 gctcgcccgc gcagcccgac gagtcctcgg cgcccgcgta tttcgcgggc aaatcctcgg
    10501 cgtcaccgcg ccgacatagc gcccgggccg ccgcgatcgc gtatcatccc gcggcgatgg
    10561 acggcgaccc gcacggccta tggatcctcg gcatcagcgt cgccgcgctg ctgctcgggc
    10621 cggcgatcca cctgctcgcg cgccgcgccc cgccgacgat ggccgcgctc gacaacttcg
    10681 tgctcgtcgc cgtcgtcggc ctcggcgcgc tggagatcct ccccggcgcg ctccacctcg
    10741 ccggcctgcc cgcgttcatc gtcctcctgc tcggcgccct cggccccgcc ctcgccgacg
    10801 gcccgctgca ccggctctcg cgcggcaccc acggcgcagc cctggccctc ggcgtcgtcg
    10861 gcatggcgat ccactcgttc accgacggcc tcgccctcgc ctccgcccac gccgccggca
    10921 accatagcca cgccctcgag gccgccgtca tcgcccacca gctcccggtc gccgtcgccg
    10981 tgtggtggct gatcgtgccg atccgacgcc tggggtggat cggcgcgacc gtcgcgatgg
    11041 tcggcctcgg gctcgccacg atcctcggct tcgagctcgc cgacgcctcc ctcggcgctc
    11101 tcacccccgc ctggctcggc ctgctccagg ccctgttcgc cggcttcctc ctccacgtca
    11161 tcgcccaccg ctcgcacccc gtcggcgagg acaccggcgc cagccgtacg atccacgtcg
    11221 ccgccgcgct cggcggcctg gtcggcctcg gcctgctcgc cctgatcggg gtcgacggca
    11281 tggacgccgg ccacgccggc cacctcgaca gcggccacgc cggcgaggcg cacggcgtgc
    11341 tgcccgacat ctttcacctc gcccgcgccg ccgccccgcc gatcgtcgcg gccctcgccc
    11401 tcgccctgtt gatcggccgg cccttgc
//
LOCUS       DASWNJ010000724         1572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_13897, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000724 DASWNJ010000000
VERSION     DASWNJ010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13897"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VGB85_12090"
     CDS             <1..791
                     /locus_tag="VGB85_12090"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0134820.1"
                     /translation="RALLGLEEAISVSVVDPLMGDDGWAFGERDGCTPDPIFGARFLR
                     SIYLRADINYSGRVTVPVLWDRERATIVSNESREILRMLDVEFAAYHRVPISLYPKSM
                     RASVDKALDAIYTPINNGVYRAGFATEQAAHEEAVGELFAALDHWDKVLEKQRFLCGE
                     VVTEADIAMFTTLLRFDPVYFGHFKCNVRRIADYPQLSNYLRDMFQLPGVAAVCRIDH
                     IKEHYYRSHPHINPTRIVPVGPALDLEAPHDRSRHGGELFATAQ"
     gene            915..>1572
                     /locus_tag="VGB85_12095"
     CDS             915..>1572
                     /locus_tag="VGB85_12095"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEY0134821.1"
                     /translation="MTATMHSASSSIVRPRMRGVFDLYAAALAIPAVAVLIAYARPGA
                     ATAAAAVYGVAMVVLLVGSALYHRPSWPVRTFLWLRKIDLANIYLMIAGTCTPLAYAL
                     TPGPGEWLMPAIWVAAAIGIAKALLWSRGHRAINAMLYVLMGTLTLPCLPALKQTVGP
                     DDFLLLATGGILYFIGAGVYALRWPNPVPVVFGYHEIFHCFVFAAAGVHYWAIWTIVT
                     R"
BASE COUNT          219 a          519 c          562 g          272 t
ORIGIN      
        1 cgcgggcgct gctcgggctc gaggaggcga tctcggtgtc ggtggtcgac ccgctgatgg
       61 gcgacgacgg ctgggcgttc ggcgagcgcg acggctgcac gccggacccg atcttcgggg
      121 cccggttcct gcgatcgatc tacctccggg ccgacatcaa ctacagcggt cgcgtgacgg
      181 tgccggtgct gtgggaccgc gagcgcgcga cgatcgtgtc caacgagtcg cgcgagatcc
      241 tgcgcatgct cgacgtcgaa ttcgcggcct accaccgcgt cccgatctcg ctgtacccga
      301 agtcgatgcg cgcgagcgtc gacaaggcgc tcgacgcgat ttacacgccg atcaacaacg
      361 gggtgtaccg ggccggcttc gccacggagc aggcggccca cgaggaggcg gtcggcgagc
      421 tgttcgccgc gctcgaccac tgggacaagg tgctcgagaa gcagcggttt ttgtgcggcg
      481 aggtcgtcac cgaggccgac atcgcgatgt tcacgacgct gctgcgcttc gacccggtgt
      541 acttcgggca cttcaagtgc aatgtccgca ggatcgcgga ctatccgcag ctgtcgaatt
      601 acctccgcga catgttccag ctgcccggag tcgcggcggt gtgccggatc gaccacatca
      661 aggagcatta ttaccgcagc cacccgcaca tcaacccgac gcgcatcgtg ccggtcgggc
      721 cggcgctgga cctcgaggcc ccgcacgacc gaagccgcca cggcggggag ctgttcgcca
      781 cggcccagtg acggtgacca caatttcccc ggcggccctc gtcattgttt ggtcatggtc
      841 ggcgggccgg gcggggtctg acgtgagatg gccccccttc acctcggcgc ggggaacgac
      901 gtaccatcgc gcccgtgact gcgaccatgc actcggccag ctcgtcgatc gtgcgcccgc
      961 ggatgcgcgg cgtgttcgac ctctatgccg ccgcgctggc gatccccgcg gtggccgtgc
     1021 tgatcgcgta cgcgcgaccg ggggccgcga ccgcggcggc ggcggtgtac ggggtggcga
     1081 tggtggtgct gctcgtcggc agcgcgctgt accaccggcc gtcgtggccg gtgcgcacgt
     1141 ttttgtggct gcgaaagatc gacctggcga acatctacct gatgatcgcc ggcacgtgca
     1201 cgccgctggc ctacgcgctg acgccggggc cgggcgagtg gctgatgccg gcgatctggg
     1261 tggcggcggc gatcgggatc gccaaggcgc tgctgtggtc gcgcggtcac cgggcgatca
     1321 acgcgatgct gtacgtgctg atgggtacgc tgaccctgcc gtgcctgccg gccttgaagc
     1381 agaccgtcgg gcccgacgac ttcctgctgc tggcgacggg cggcatcttg tacttcatcg
     1441 gcgccggggt gtacgcgcta cgctggccga acccggtgcc ggtggtgttc ggttaccacg
     1501 agatcttcca ttgcttcgtg ttcgcggccg cgggcgtgca ctactgggcg atctggacga
     1561 tcgtgacgcg gt
//
LOCUS       DASWNJ010000725         3136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_438529, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000725 DASWNJ010000000
VERSION     DASWNJ010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438529"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(645..1613)
                     /locus_tag="VGB85_12100"
     CDS             complement(645..1613)
                     /locus_tag="VGB85_12100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0134822.1"
                     /translation="MAVVAWVAWAAWTCSPARAGSLGIWYTRPARFADAGRRRFKKAG
                     KTRYVRGMDLRDHVAIVTGASSGIGLAVARMLIASGAKVAMVARGAERLEHACTTLGP
                     RATAFALDVGDLAALTALPGQVVERLGRLDIVVNNAGLHYRGDMLRHSATQLAEMFMV
                     NLVAPVVLTRVAADLMTAGGVFVNVASLAGKLGVPGSAVYSGTKAGLRFWALAAAEDL
                     GRRGLRIANVNPGPVDTGFFDDDIENVSNLTFSQPMSSAEQVAAAVMDCVRGGATGLD
                     VDVPYLSGKLSTLGYLSPGLGRLLRPLLEMRGAAAKRQYMARKGQG"
     gene            complement(1570..>3136)
                     /gene="groL"
                     /locus_tag="VGB85_12105"
     CDS             complement(1570..>3136)
                     /gene="groL"
                     /locus_tag="VGB85_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969504.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY0134823.1"
                     /translation="KVTLGPRGRNVVIEKSWGSPTVTKDGVTVAKEIELEDKFENMGA
                     QMVKEVASKTSDLAGDGTTTATVLAQAIYREGLKMVAAGHDPMELKRGMDQAVEAVIA
                     HIRDNQSKATSSDDEVAQVGTISANGDEEIGKLIAQAMSKVGKEGVITVEENKANTTD
                     LDVVEGMQFDRGYLSPYFITDQERMVVSLSDAYVLTYEKKVSTMKDLLPILEQVAKQG
                     RSLLIIAEDVDGEALATLVVNKIRGTLQIAAVKAPGFGDRRKEMLKDIAVLTGGKALT
                     EDLGEKLENLSLKDLGTAKQITIDKDNCTIVDGGGKKDDIKARVKQIRAQIEDTTSDY
                     DREKLQERLAKLVGGVAVIKVGAASEVEMKEKKARVEDALHATRAAVEEGIVPGGGVA
                     LIRAQKTLEDLKVSTHEQQVGVSIIKRAIEEPLRQIVTNAGGEPSVVMNAVRAGEAGY
                     GFNARTGKYVDLIKDGVIDPTKVVRTALQNAASVAGMMLTTEAMIAEKPKKESAAPAG
                     GGGGMGGMGGMDM"
BASE COUNT          446 a         1121 c         1021 g          548 t
ORIGIN      
        1 gcctggctcg agcggcttcc gcagaccatc agcgagccga caggacgtgc tcacgtccgc
       61 cctcgggcag ccttcgcttc acttcgcttc gcgcagacgc tgctgttggt ggcccacgcc
      121 gatcatcaat cgtttgcggt gttctcttca cccgcatgtt ggaccgccgc tgcatcttgg
      181 cggcgagctc gggctcggtc tccgctcgtg gctcattgtc cgctcggtgc tcgagctggc
      241 cgccttgcgt cacgagcacg cctgccactg gcgagcctgg ccctcggcca acaagcgtcg
      301 tgcactgcgg cgggatcacg agctggcctt cgggacagga tctcgaagcg gtgacaagcg
      361 gggcgcagac gatgccaccc gggcgacgag agggccgacg gcgggggaca caggtggagg
      421 ggacccatgg ctcgcgcgca gccgctcgaa ccaatcgtcg cggtcagaac ccctcggcag
      481 gtggcttgca gcggtgcggg gcttgatgcg tacctggcgg cgagcacgtg ccatggggag
      541 gcgccgccgt cgtgcgggac ccgcagccgt cggccctccc gtcgtccgcc cacagccccc
      601 gagccccgcg atgcccggac ccgccagccg cagccccgcg cggcctagcc ctgcccctta
      661 cgagccatgt attgccgctt ggccgccgcc ccccgcatct cgagcaacgg ccgcagcagc
      721 cgcccgagcc ccggcgacag atatcccaat gtcgacagct ttccagacaa atacggcaca
      781 tcgacatcga gcccggtcgc cccgccgcgc acgcaatcca tcaccgccgc ggcgacctgc
      841 tccgcgctcg acatcggctg cgagaacgtc agattcgaca cattttcaat gtcgtcgtca
      901 aagaacccgg tatcgacggg cccgggattg acgttcgcga tccgcagccc ccgacgcccg
      961 aggtcctccg cggccgccag cgcccagaag cgcagcccag ccttggtccc cgagtacacc
     1021 gccgagcccg gcaccccgag cttgccggcc agactggcga cattgacgaa cacgcccccg
     1081 gcggtcatca ggtcggccgc gacgcgcgtc agcaccacgg gtgcgacgag gttgaccatg
     1141 aacatctcgg ccagctgcgt cgccgagtga cgcagcatgt cgccgcgata atgcagcccc
     1201 gcgttgttga cgacgatgtc gagccgaccg aggcgctcga ccacctggcc cggcagggcc
     1261 gtcagcgccg cgagatcgcc gacatcgagc gcaaacgcgg tcgcgcgcgg cccgagggtc
     1321 gtgcacgcgt gctccagccg ctccgcgccg cgggcgacca tcgcgacctt ggcccctgaa
     1381 gcgatcagca tgcgggcgac ggccagaccg atgcccgagg aggcgcccgt gacgatggcg
     1441 acgtggtctc gcaggtccat gccgcgaaca tagcgcgttt tgcccgcctt tttaaaacga
     1501 cgacgccccg cgtcggcgaa ccgagcgggg cgcgtgtacc agatcccaag agatccagcg
     1561 cgggccggac tacatgtcca tgccgcccat gccacccatg ccaccaccgc caccggccgg
     1621 cgcagccgac tccttcttgg gcttctcggc gatcatcgcc tcggtggtga gcatcatgcc
     1681 ggccacggag gcggcgttct gcagcgcggt gcggacgacc ttcgtcgggt cgatgacgcc
     1741 gtccttgatc aggtcgacgt acttgccggt gcgggcgttg aagccgtaac cggcctcacc
     1801 cgcgcggaca gcgttcatca ccacgctcgg ctcgccgccg gcgttggtga cgatctggcg
     1861 caggggctcc tcgatggcgc gcttgatgat gctgacgccg acctgctgct cgtgggtcga
     1921 gaccttgagg tcctcgaggg tcttctgggc gcggatgagg gccacaccgc cgccggggac
     1981 gatgccctcc tcgacagccg cgcgggtcgc gtgaagggcg tcctcgacgc gggccttctt
     2041 ctccttcatc tcgacctcgg acgccgcgcc gaccttgatc acggcgacgc cgccgaccag
     2101 cttggcgagg cgctcttgca gcttctcacg gtcgtagtcc gaggtggtgt cctcgatctg
     2161 cgcgcggatc tgcttgacgc gggccttgat gtcgtccttc ttgccgccgc cgtcgacgat
     2221 cgtgcagttg tccttgtcga tcgtgatctg cttggcagtg ccgaggtcct tgaggctcag
     2281 gttctcgagc ttctcgccga ggtcctcggt gagcgccttg ccgccggtca gcacggcgat
     2341 gtccttgagc atctccttgc gacggtcgcc gaagcccggg gccttgacgg ccgcgatctg
     2401 cagcgtgccg cggatcttgt tgaccacgag cgtcgcaagc gcctcgccgt cgacgtcctc
     2461 ggcgatgatc aggaggctgc ggccctgctt ggcgacctgc tcgaggatcg gcaggaggtc
     2521 cttcatcgtc gagaccttct tctcgtaggt caggacgtag gcgtcgctca gcgacacgac
     2581 catgcgctct tgatcggtga tgaagtacgg gctcaggtag ccgcggtcga actgcatgcc
     2641 ctcgacgacg tcgaggtcgg tcgtgttggc cttgttctcc tcgaccgtga tcacgccctc
     2701 cttgccgacc ttgctcatcg cctgcgcgat cagcttgccg atctcctcgt cgccgttggc
     2761 ggagatcgtg ccgacctgcg cgacctcgtc gtcgctgctg gtcgccttcg actggttgtc
     2821 gcggatgtgg gcgatgacgg cctcgacggc ctggtccatg ccgcgcttga gctccatcgg
     2881 gtcgtgaccc gcggcgacca tcttcaggcc ctcacggtag atcgcctggg cgagcaccgt
     2941 cgcggtcgtc gtgccgtcac cggcgaggtc ggaggtcttc gaggcgacct ccttgaccat
     3001 ctgggcgccc atgttctcga acttgtcctc gagctcgatc tccttggcga cggtgacgcc
     3061 gtccttggtg accgtggggg acccccagga cttctcgatc acgacgttgc ggccgcgcgg
     3121 cccgagggtg accttg
//
LOCUS       DASWNJ010000726         1705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_119052, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000726 DASWNJ010000000
VERSION     DASWNJ010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119052"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VGB85_12110"
     CDS             <1..465
                     /locus_tag="VGB85_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134824.1"
                     /translation="KPRVVEIRCPHQPVARRVATAVAPFPEVLYAQLIELPAAIGDGL
                     DGELAAWHRTLLAEGALPPELWNTVQGLVNRAVRRELGPEPARAYMRAVDLRAGAYRE
                     PWSMGESRFIANANAHKTYQHRRAYLEPHTGGHPNGRLVKRWPDGPALEDHD"
     gene            complement(499..1554)
                     /locus_tag="VGB85_12115"
     CDS             complement(499..1554)
                     /locus_tag="VGB85_12115"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEY0134825.1"
                     /translation="MHPDGICIVGLGARTPVGRSWPGSAAAVRASVVRFEEHPLYTDP
                     NGRRLVVSRADWLDPARDASPHLSALAVPAAREALLDLPRSNVRTWLALPSLRPGRPD
                     DLEQVLSRRLRDKLGETGHTLDIARVIAGDHSAGVAALALACEDLREGRVELALVGGV
                     DSLLEFDTLDWLISEQRVRTINNPRGFIPGEGAGFVLLATPEAAARRGLPVLGRVLAV
                     SLAHEAMHARDAVNTGVGLTRAALASLAALPPGTKVATRYCDLNGEPHRASEYGFATV
                     RISDRLERADSFVAPAECWGDVGAATAPLLLGLAVAAARRGHAAGTHSLLTTSAGGGL
                     CGAVLVHAQVEPGPEFL"
BASE COUNT          243 a          590 c          659 g          213 t
ORIGIN      
        1 aagccgcgcg tggtcgagat ccgttgcccg catcagccgg tcgcgcgccg ggtcgccacc
       61 gcggtcgcgc cgttccccga ggtgttgtat gcgcagctga tcgagttgcc ggctgcgatc
      121 ggggacgggc tcgacggcga gctcgcggcc tggcaccgga cgctgctcgc cgagggtgcc
      181 ctgccgccgg agctgtggaa caccgtgcag gggctcgtca atcgcgccgt gcggcgcgag
      241 ctcgggcccg agccggcgcg tgcgtacatg cgcgcggtcg acctgcgcgc cggggcctac
      301 cgcgagccgt ggagcatggg cgagagccgc ttcatcgcca acgcgaacgc ccacaagaca
      361 tatcaacatc ggcgcgcgta cctcgagcca cacaccggcg gccaccccaa cggccggctg
      421 gtcaagcgct ggcccgacgg accagcgctc gaggatcacg attgagggta cctgcccctt
      481 cgggcaggtt cgatattttt atagaaattc ggggccgggc tcgacctgcg cgtgcacgag
      541 cacggcgccg cacaggccgc cgccggcgga ggtcgtcagc aggctgtggg tgccggccgc
      601 gtggccgcgc cgggccgccg cgacggcgag cccgagcagc agcggggcgg tcgcggcccc
      661 gacatccccc cagcactcgg ccggcgcgac gaacgagtcg gcccgctcga gccggtcgga
      721 gatccgcacg gtggcaaagc cgtattcgct ggctcggtgc ggctcgccgt tgaggtcgca
      781 gtatcgcgtc gccaccttgg tgccgggcgg cagcgctgcg aggctggcga gcgcggcgcg
      841 ggtcaggccg acgccggtgt tgacggcgtc gcgcgcgtgc atcgcctcgt gcgcgagcga
      901 gacggcgagc acgcgcccga gcaccggtag accccgccgc gcggccgcct cgggcgtggc
      961 gagcaggacg aagcccgcgc cttcaccggg gatgaagccg cggggattat tgatggtacg
     1021 cacgcgctgc tcggagatca gccagtcgag ggtgtcgaac tcgagcaggc tgtcgacgcc
     1081 gccgacgagc gcgagctcga cacggccctc gcgcaggtct tcacaggcga gggcgagcgc
     1141 cgccacgccg gcgctgtggt ccccggcgat cacgcgcgca atatcgaggg tgtggccggt
     1201 ctcgccgagc ttgtcgcgca ggcggcggga cagcacttgc tcgaggtcgt cgggacgtcc
     1261 ggggcgcagc gagggcaggg cgagccaggt gcggacattg ctgcgcggca ggtcgagcag
     1321 cgcctcgcgg gcggccggta cggcgagcgc cgacaggtgc gggctcgcgt cgcgggccgg
     1381 gtcgagccag tcggcgcgcg agacgacgag gcgccggccg ttggggtcgg tgtagagcgg
     1441 gtgctcctcg aagcggacca ccgaggcgcg caccgccgcg gccgagcccg gccacgagcg
     1501 accgaccggc gtgcgcgcgc cgaggccgac gatacaaata ccatcaggat gcacgcaggg
     1561 tctccagggc gacgtccgca gatatcaccg cgatctcggt gctgcgcagg gcatacagcg
     1621 tcttgtggca ctcgagcgcg ctgtgccaca cgacgacgag ccggccggcg tcggcgtcga
     1681 tctcgacggt gcggatctcc gggcg
//
LOCUS       DASWNJ010000727         3140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_119061, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000727 DASWNJ010000000
VERSION     DASWNJ010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119061"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..334
                     /locus_tag="VGB85_12120"
     CDS             35..334
                     /locus_tag="VGB85_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134826.1"
                     /translation="MPIDDLRTRTPAARPADEADEAHDIDDDLEDIDHGGEPNPTPEQ
                     LAEIEAAWIAEAQEAMKALDEGHEVAIPWEEARVLIFAPLTPEELAAVAPRRARR"
     gene            354..671
                     /locus_tag="VGB85_12125"
     CDS             354..671
                     /locus_tag="VGB85_12125"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY0134827.1"
                     /translation="MPAAQSPDKYRIIVRERAWLALRRATHWYEQRNTVAAARFVMAI
                     DNAVASLAEAPLRWALWMPDVRRCLLPGFPYSPLYRVVGTQVQVLAVVHHARHPDTWR
                     AGT"
     gene            701..1840
                     /locus_tag="VGB85_12130"
     CDS             701..1840
                     /locus_tag="VGB85_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0134828.1"
                     /translation="MTPVAMTDDERVGFRTTLRGFVERELMPHIEAWEEAGEFPRDLY
                     LRAGELGLLGMGFPEEYGGSAGDSTSVIALCEELARAGSGGLIAGLFSHGIGLPPVLN
                     LGDDEQKWRFVPDVLAGRKISALAITEPSGGSDVANLRTVARRDGDHYIVHGSKMFIT
                     SGMRADLVTVAVRTGPPGLGGISLLVVERDTPGFTQTPLKKMGWWCSDTAALYFDECR
                     VPVANRLGEENAGFLGIMQNFNRERLMLAAMAVAFSQVCYDDAVAYARMRETFGKPLI
                     SRQVIRHKLVDMATRIEPVRCWLYDLARRVDGGEWPIAQICMLKNAATACVEHCAREG
                     VQILGGAGYLRGARVERIFRETKVLAIGGGAEEIMKDLAARQLGW"
     gene            1877..>3140
                     /locus_tag="VGB85_12135"
     CDS             1877..>3140
                     /locus_tag="VGB85_12135"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0134829.1"
                     /translation="MAADTIPSRFFSQGDAHPDTPAAHTRTDGAWRTLTWREYAGLTR
                     RVARALIALGVEAGHATCILGFNRVEWVTFAIGTQAVGAAPAGIYTTCSPTEVQYIVH
                     HAEAAVVLVENAAQFAKLAQERERLPYLRHVVLMQGARVDDPLALTWDEFLARGDATT
                     DAAVDARLRDIPEDATATYIYTSGTTGPPKAVMLSHRNVLWTADAIASLMAFTPADTM
                     LSYLPLSHIAEQLISMYGPVVLGNQVYFAESLEKLPDNLKEVQPTIFFGVPRIWEKFH
                     AAVRGKLAQVTGPKKHLLAWAMGVAKRVHAHGNRGEAPPALLGLQYQLAQRLVFRKLK
                     TALGLGRVKVCSSGAAPIAKEVLEFFSGLDIVVYEVYGQSEDTGPTSTNYPGATRFGS
                     VGRPLVGVEVRLGDDGEILVRGANVFKGY"
BASE COUNT          476 a         1145 c         1024 g          495 t
ORIGIN      
        1 ggcccctccc acgcccgcgt ggtagcgtcg agccatgccc atcgacgacc tccgcacccg
       61 cacgcccgcg gcgcgccccg ccgatgaagc cgatgaagcc cacgacatcg acgacgatct
      121 cgaggacatc gaccatggtg gcgagccgaa cccgacgccg gagcagctcg cagagatcga
      181 agccgcctgg atcgcggagg cccaggaggc catgaaggcg ctcgacgagg gccacgaggt
      241 cgctatcccg tgggaggagg cgcgcgtcct catctttgct ccgctcacgc ccgaggagct
      301 cgcagcagtg gctccgcgac gggcgcgacg atgatcgtcg agtaatctgc tcggtgcccg
      361 ccgctcaatc tccggacaag tatcggatta tcgtccgcga gcgtgcctgg ctcgccctcc
      421 gtcgcgcgac gcactggtac gagcagcgca acaccgtcgc cgccgccagg ttcgtcatgg
      481 cgatcgataa cgccgttgca tcgctggccg aggcgccgct gcgatgggcc ctctggatgc
      541 ctgacgtgcg tcgctgcttg ctgcccggct tcccgtattc gcccctttat cgcgtcgtgg
      601 gcacgcaggt ccaggtcctc gccgtcgtcc accacgcccg tcatcctgac acctggcgcg
      661 cgggcacatg agccctcatc cctgatattc tccccgccag atgacccccg tggcgatgac
      721 ggacgacgag cgtgtcgggt ttcggaccac gctgcggggc ttcgtcgagc gcgagctgat
      781 gccgcacatc gaggcctggg aggaggccgg cgagttcccg cgcgacctgt accttcgggc
      841 aggtgagctc ggcctgctcg gcatgggctt cccggaggag tatggcggca gcgccggcga
      901 cagcacctcc gtgatcgcgc tgtgcgagga gctcgcgcgc gccggctcgg gcgggttgat
      961 cgcaggcctg ttcagccacg gcatcggtct gcctccggtc ctcaacctcg gcgacgacga
     1021 gcagaagtgg cgctttgtcc cggacgtgct cgccggtcgc aagatctccg cgctcgccat
     1081 caccgagccc tcgggcggct ccgatgtcgc taacctcagg accgtcgccc ggcgcgacgg
     1141 tgatcattac attgtccatg gctcgaagat gttcatcacc tcgggcatgc gcgccgacct
     1201 ggtgaccgtc gccgtgcgca ccggcccgcc ggggctcggg ggcatctcgc tgctcgtcgt
     1261 cgagcgcgac acccccggct tcacccagac gccgctgaag aagatgggct ggtggtgctc
     1321 cgacaccgcc gcgctgtact tcgacgagtg tcgcgtcccc gtcgccaacc ggctcggcga
     1381 ggagaacgcc ggcttccttg gtatcatgca aaacttcaac cgcgagcgcc tgatgctcgc
     1441 ggcgatggcg gtcgcgttct cgcaggtctg ttacgacgac gcggtcgcct acgcgcgcat
     1501 gcgcgagacc ttcggcaagc cgctgatcag ccgccaggtg atccgtcaca agctggtcga
     1561 catggcgacg cgcatcgagc cggtgcgctg ctggttatac gacctggcgc gccgcgtcga
     1621 cggcggtgag tggccgatcg cgcagatctg catgctcaag aacgccgcga ccgcctgcgt
     1681 cgagcactgc gcccgcgagg gcgtgcagat cctcggtggc gccggttacc tgcgcggcgc
     1741 ccgggtcgag cggatcttcc gcgagaccaa ggtgctggcg atcggcggcg gcgccgagga
     1801 gatcatgaag gacctcgccg cgcgtcagct cggctggtga acattcgtcg tgcatcacaa
     1861 ccccggagag cagcccgtgg ccgcagatac catcccatct cgcttcttct cgcagggtga
     1921 cgcccacccc gacactcccg ccgcgcacac gcgcacggac ggcgcctggc gcacgctgac
     1981 gtggcgcgag tacgcgggtc tgacgcgcag ggtcgcccgc gccctgatcg cgctcggcgt
     2041 cgaggccggc cacgccacct gcatcctcgg cttcaaccgc gtcgagtggg tcacctttgc
     2101 gatcggcacc caggccgtcg gcgccgcccc ggccggcatc tacacgacct gctcgccgac
     2161 ggaggtgcaa tacatcgtac accacgccga ggccgccgtc gtgctcgtcg agaacgccgc
     2221 gcagttcgcc aagctcgcgc aggagcgcga gcgcctgccg tacctgcgcc acgtcgtgtt
     2281 gatgcagggc gcccgcgtcg acgacccgct cgcgctcacc tgggacgagt tcctggcccg
     2341 cggcgacgcg accaccgacg ccgccgtcga tgcccgcctg cgcgacatcc ccgaggacgc
     2401 gaccgccacc tatatctaca cctccggcac cacgggtccg cccaaggcgg tgatgctcag
     2461 ccaccgcaac gtgctgtgga ccgccgacgc gatcgcgtcg ctgatggcgt tcacgccggc
     2521 ggacacgatg ctctcctatc tgccgctgtc acacatcgcc gagcagctga tctcgatgta
     2581 cggcccggtc gtgctcggca atcaggtcta cttcgccgag tcgctcgaga agctcccgga
     2641 taacctcaag gaagttcagc cgacgatctt cttcggcgtg ccgcggatct gggagaagtt
     2701 ccacgccgcc gtgcgcggca agctggccca ggtcaccgga ccgaagaagc acctgctcgc
     2761 gtgggccatg ggcgtcgcaa aacgcgtgca cgcccacggc aaccgcggcg aggccccgcc
     2821 cgccctgctc ggcctccagt atcagctcgc gcagcgcctg gtgtttcgca agctcaagac
     2881 cgccctcggg ctcggccgcg tcaaggtgtg cagctccggc gccgccccga tcgcaaagga
     2941 ggtgctcgag ttcttcagcg gcctcgacat cgtcgtgtac gaggtctacg gccagtccga
     3001 ggacaccggt ccgacctcga ccaactatcc cggtgcgacg cgcttcggca gcgtcggccg
     3061 gccgctcgtc ggcgtcgagg tccggctcgg cgacgacggc gagatcctcg tgcgcggcgc
     3121 gaacgtcttc aagggttatt
//
LOCUS       DASWNJ010000728         6489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_398070, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000728 DASWNJ010000000
VERSION     DASWNJ010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6489
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398070"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1727)
                     /locus_tag="VGB85_12140"
     CDS             complement(<1..1727)
                     /locus_tag="VGB85_12140"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEY0134830.1"
                     /translation="MPSGLTPWRARQRGSCLAGAILLAGCYGGDETLDAGDETLDATA
                     ASSTPTTTPTGEDSTADGGSDDGGPILPDFTPGPPVLPRLTQQQYFNSIEALLGPGLP
                     TPQLEPDTNPYLFFNIGAASTTLSENGVQQYETAADLFTTIIFNDPARREDLVGCLPA
                     APGDACVAQFLGKFGRRAFRRPLSPEETTRWLGMATELASGDPWQGLRHAVAGMLQSP
                     NFLYRVELGEPDVGDPTRLRYTTFEMASRLSFLLWNTTPDDALLDAAAVGDLLTEAGL
                     AAETERLLKDPRAHQAVRDFFTQFLDLGRLDGIARDPERYPLFSPTMIDSMRTEVRLL
                     VDDLIYRQNTDIRGIFSTRHTFVNSELAALYGVPAEGATPITFVPIDLPEDGPRAGLL
                     TLGAFLTMNAHEIDTSPTARGKYVRTRVLCQDVPAPPDDADFNLDPVEGQGPQTLRQR
                     LEQHRSDPTCAGCHAFMDPPGFLFENFDSIGAFRTLDNGLPIDATGDLDGIPLQNARG
                     LAAMLEDDARVGRCIVTQLYRHAIGRRDILSEKPALDEIDARFADEGYRFTELLKILV
                     TSDLFRTVA"
     gene            complement(1884..2252)
                     /locus_tag="VGB85_12145"
     CDS             complement(1884..2252)
                     /locus_tag="VGB85_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134831.1"
                     /translation="MRNGPLSRCIFLAGACMGAVRCGQSELSEQCAVICQSFACDDGV
                     VPDCKSDCIGRMDDAEAIGDACAKTYDVMLDCLAEVECGIISHWELRRGGSLDYPCKA
                     ETDLFNNECPGLWFDRNVKE"
     gene            complement(2258..3319)
                     /locus_tag="VGB85_12150"
     CDS             complement(2258..3319)
                     /locus_tag="VGB85_12150"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEY0134832.1"
                     /translation="MAVKLRRVRTPLRARLRFAVYAARLLGGRLLVLAALLGLGGLLL
                     QRRGDPAVVDHLDFIGACFLVYTQLFFEHLTALPDDWALRIMYFTVPIAGVFILAEGL
                     LKVGTSLLDFNNHRQAWVRIMAETYRDHIVLIGLGHVGFRVLDELLHRGRQVIAVESE
                     DSGQFVEEARARGVPLIIGDARRESLLRDLGIEHAACVIACTDNDLVNLEVALDARHL
                     NPKIRLVMRFFDQSMAQKLGKAFTVDSTFSTSALSAPVFAAAALDEQVLGAYRMGDTV
                     MASVQIDVAAGTTLVGKTLRDAEEVLDAAVVGVRRQGQLPSHKFSRSEALRPGDALVC
                     HVAVEEIAGVKTRACVRVA"
     gene            complement(3428..5575)
                     /locus_tag="VGB85_12155"
     CDS             complement(3428..5575)
                     /locus_tag="VGB85_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134833.1"
                     /translation="MTTRLSLLLTGVCVFATACSDGGRDSGTDNNTDSGTAGVSISGT
                     TSGDTDDTPTTATPTSQPSDPSGGTPGTASEGSATEVTGDTTATDPTTVSTTMTSDDT
                     TTGDDPIVEVQIDPLDAIVTVVDGQIPGAMQYTAKGITEGGLEVPITGTWAYDRLDLA
                     SLGAQSGQFAVTGFAGGKGTVTFDPAGPLGPVSTSASVKLQFSSEPMPVDPGVKDGFG
                     FATLPDPAMQLVYPYDNTVFPRGLTGPVVQWNGGGAADVYYIHVYSDYFELKAYTTVP
                     PPSRYSFPAMPVDVWLKLTASTEGPVKFDIQRFDGVTHYTAKQQTWTIAPANLTGAVY
                     YWEVNNGNVVRLNIGDTGPQQFIQKPPGVQCVACHSVSKDGSRIAAAFHGDYSPWNTI
                     DAATGVVEYFPDIASGFQAISPNGSHVIWGQSDQTGALKLTTFNDKTVLSTLTTPGGM
                     PVHPVWSGDGAKVAFARRTDGNWLDFNNSELWLAEIDLMTNTFSNVTQIVNKTPPLGT
                     TTFPTFAPDSQWIAFNRLNQARTRAAVTEVWMTNLDGSAQMRLDNANGLGIIEPGQDQ
                     TTYEPTFLPVSVGGYYWLIIGSERKYGNTLTDTNPGSRRKQLWVTAIDANIEPGKDPS
                     HPAFWLPGQELNNSNMRGEWALAPCKQIGEGCNAGFDCCGGFCYPPAEGEMPTCNDEP
                     DTCSHLGDSCEVDAACCEGEGTCIGGFCSIMPG"
     gene            complement(5778..>6489)
                     /locus_tag="VGB85_12160"
     CDS             complement(5778..>6489)
                     /locus_tag="VGB85_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134834.1"
                     /translation="RAAVPDVLAPPRSRRTKLLDAFHDVRARRGALPAIAGGLALLLT
                     CGLLFSDDGEDRVLDDEVASVAAEGAEPPDPTPAATPVTTPDTTAKPPARADAKPPQP
                     PPAVPAPVGDEPPLPPTLAQPLATLLDGEEKRARKRAAEAVLEHKPKEAVPAYALNLA
                     WLEKASSCGNKRGVLEKMEIAADPRVLPALKRLAHTRRKGCGFFGSQDCLDCLRETLA
                     RTIGRLEAEASAAAILAP"
BASE COUNT          937 a         2038 c         2509 g         1005 t
ORIGIN      
        1 tcggcgacgg tgcgaaacag gtcgctggtc acgaggatct tcagcagttc ggtgaaacgg
       61 tagccctcgt cggcgaagcg cgcgtcgatc tcgtcgagcg cgggcttctc ggacaggatg
      121 tcgcggcggc cgatcgcgtg gcggtacagc tgggtgacga tgcaacgacc gacgcgcgcg
      181 tcgtcctcga gcatcgccgc gaggccgcgg gcgttttgca gcgggatgcc gtcgaggtcg
      241 ccggtggcgt cgatcgggag cccgttgtcg agcgtacgaa aggcgccgat cgagtcgaag
      301 ttctcgaaca aaaagccggg cgggtccatg aaggcgtggc agccggcgca ggtcgggtcg
      361 gagcggtgct gctcgaggcg ctggcgcagc gtctgtggac cctggccctc gacggggtcg
      421 aggttgaagt cggcgtcgtc gggcggggcg gggacgtcct gacacagcac gcgcgtgcgc
      481 acgtacttgc cgcgcgcggt cggggaggtg tcgatctcgt gggcgttcat cgtcaggaag
      541 gcgccgaggg tcagcaggcc ggcgcgcggg ccgtcctcgg gcaggtcgat cggcacgaag
      601 gtgatcggcg tggcgccctc ggccgggacg ccgtagaggg ccgccagctc gctgttcacg
      661 aaggtgtggc gggtgctgaa gatcccgcgg atgtcggtgt tctggcggta gatgaggtcg
      721 tcgacgagca gccgcacctc ggtgcgcatc gagtcgatca tcgtcggcga gaacagcggg
      781 tagcgctcgg ggtcgcgggc gatgccgtcg aggcggccga ggtcgaggaa ctgcgtgaag
      841 aagtcgcgca ccgcctggtg tgcgcgcggg tccttgagca ggcgctcggt ctccgcggcg
      901 aggccggcct cggtcagcag atcgccgacg gcggcggcgt cgagcaacgc gtcgtccggg
      961 gtcgtgttcc acagcaggaa cgacagccgg ctcgccatct cgaaggtggt gtagcgcagg
     1021 cgggtcgggt caccgacgtc gggctcgccg agctcgacgc gatacaggaa gttgggcgac
     1081 tgcagcatgc cggcgacggc gtggcgcagg ccctgccagg ggtcgccgct ggcgagctcg
     1141 gtcgccatgc cgagccagcg ggtcgtctcc tcggggctca acggccggcg aaacgcgcgg
     1201 cgtccgaact tgccgaggaa ctgcgcgacg caggcgtcgc cgggggcggc gggcaggcag
     1261 ccgacgaggt cctcacgccg cgcggggtcg ttgaagatga tcgtcgtgaa caggtcggcg
     1321 gcggtctcgt actgctggac cccgttctcg gacagcgtcg tgctcgcggc gccgatgttg
     1381 aagaacaggt aggggttggt gtcgggctcg agctgcgggg tcggcaagcc ggggccgagc
     1441 agcgcctcga tactattaaa atattgctgc tgggtcaggc gcggcagcac cggcggaccg
     1501 ggcgtgaagt ccggcaggat cgggccgccg tcgtcactgc caccgtcggc cgtcgagtcc
     1561 tcgcccgtcg gcgtggtcgt cggggtcgag gacgcggcgg tggcgtcgag cgtctcgtcg
     1621 ccggcgtcga gcgtctcgtc gccgccgtaa cagccggcga gcaggatggc ccccgcgagg
     1681 caactaccgc gctgccgagc gcgccaagga gtcagaccag atggcatccg cacgatgaca
     1741 ctcatcgcat ggtttgcagc gcctgtggag agggcgcgcg gccgtgggct cacacccccg
     1801 gggcgcgggc tgggtggcag acaccggacg caaagttccc tgaaagcgcg gacggccccg
     1861 tcgtccgggc ggcgtccgct gcgtcactcc ttgacgttgc ggtcgaacca cagacccggg
     1921 cactcgttat tgaacagatc ggtctcggcc ttgcacggat aatcgagcga cccgccgcgc
     1981 cgcaactccc agtggctgat gatcccacac tccacctcgg ccaggcaatc gagcatgaca
     2041 tcgtaggtct tggcgcacgc gtccccgatt gcctccgcgt cgtccatccg gccgatgcag
     2101 tcgctcttgc aatccggcac gacaccgtca tcgcaggcaa acgactggca gatgaccgcg
     2161 cattgctccg agagctcgct ctgcccgcat ctcaccgcgc ccatgcaggc gccggcgagg
     2221 aagatgcatc ttgataatgg cccgttgcgc atgggtctca cgcgacgcgc acgcaggcgc
     2281 gggtcttcac gccggcgatc tcctcgacgg cgacgtggca gacgagcgcg tcgccagggc
     2341 gcagggcctc gctgcgcgag aacttgtggc tcggcagctg gccctggcgg cgcacgccga
     2401 cgacggcggc gtcgagcact tcttcggcgt ctcgcaaggt cttgccgacg agggtggtgc
     2461 cggcggcgac gtcgatctgc acgctggcca tgacggtgtc gcccatgcgg taggcgccga
     2521 gcacctgctc gtcgagggcg gcggcggcga acacgggggc ggagagggcg gaggtcgaga
     2581 aggtcgagtc gacggtgaac gccttgccga gcttttgggc catgctctgg tcgaagaagc
     2641 gcatcacgag gcggatcttc gggttgaggt ggcgggcgtc gagggcgacc tcgaggttga
     2701 cgaggtcgtt gtcggtgcag gcgatcacgc acgcggcgtg ctcgatgccg aggtcgcgca
     2761 gcagggactc gcggcgggcg tcgccgatga tcagcgggac gccgcgggcg cgcgcctcct
     2821 cgacgaattg cccgctgtcc tcggactcga cggcgatcac ctggcggccg cggtggagca
     2881 gctcgtcgag cacgcgaaag ccgacgtggc cgaggccgat caggacgatg tggtcgcggt
     2941 aagtctcggc catgatgcgc acccatgcct ggcgatggtt gttgaagtcg agcagcgagg
     3001 tgccgacctt gagcaggccc tcggcgagga tgaagacgcc ggcgatcggg accgtgaagt
     3061 acatgatccg cagggcccag tcgtcgggca acgcggtcaa gtgctcgaag aacagctgcg
     3121 tgtagaccaa aaagcaggcg ccgatgaagt cgaggtggtc gacgaccgcc gggtcgccgc
     3181 gtcgctgcaa cagcaggccg ccgagaccca gcaaggcggc gaggaccagc agccgaccgc
     3241 cgagcagccg cgccgcatag acggcgaagc gcaggcgcgc gcgcagcggt gtccggaccc
     3301 ggcgcagctt gacggccatc gccggcacgc taccgcggcg cggcggggct gccaagaccg
     3361 acgcgcaaag cagtaccccc gggccagcct ggcccgaggg tcatgttcgc gtcacgcggc
     3421 gcgggcgtca gcctggcatg attgagcaga agccgccgat gcaggtgccc tcgccctcgc
     3481 agcaggccgc gtcgacctcg caggagtcgc cgaggtgcga gcaggtgtcg ggctcgtcgt
     3541 tgcaggtcgg catctcgccc tcggcgggcg ggtagcagaa gccgccgcag cagtcgaagc
     3601 cggcgttgca gccctcaccg atctgcttgc acggcgcgag cgcccactcg ccgcgcatgt
     3661 tgctgttgtt gagctcctgg ccgggcagcc aaaacgccgg gtggctcggg tccttgccgg
     3721 gctcgatgtt ggcgtcgatc gcggtgaccc acagctgctt gcggcggctg ccggggttgg
     3781 tgtcggtcag cgtgttgccg tacttgcgct cggagccgat gatcagccag tagtagccgc
     3841 cgacggagac cggcaagaag gtcggctcgt aggtggtctg gtcctggccg ggctcgatga
     3901 tgccgaggcc gttggcgttg tcgaggcgca tctgcgcgga gccgtcgagg ttggtcatcc
     3961 agacctcggt gacggcggcg cgggtgcggg cctgattgag ccggttgaac gcgatccact
     4021 gcgagtccgg cgcgaaggtc gggaaggtgg tcgtgccgag cggcggggtc ttgttgacga
     4081 tctgcgtgac gttcgagaag gtgttcgtca tcaggtcgat ctcggccagc cacagctcgc
     4141 tgttgttgaa gtcgagccag ttgccgtcgg tgcgccgcgc gaacgccacc ttggcgccgt
     4201 cgcccgacca cacgggatgc acgggcatgc cgccgggggt ggtcagcgtc gacagcacgg
     4261 tcttgtcgtt gaaggtcgtc agcttgagcg cgccggtctg gtcggactga ccccagatga
     4321 cgtgggagcc gttgggcgag atcgcctgga agccggaggc gatgtccggg aagtactcga
     4381 cgacgccggt cgccgcgtcg atcgtgttcc acgggctgta gtcgccgtgg aaggcggcgg
     4441 cgatccgcga gccgtccttg gagacgctgt gacaggcgac gcactggacg ccggggggct
     4501 tctggatgaa ctgctgcggg ccggtgtcgc cgatgttgag gcgcacgacg ttgccgttgt
     4561 tgacctccca gtagtagacg gcgccggtca ggttggcggg cgcgatcgtc caggtctgct
     4621 gtttggcggt gtagtgcgtg acgccgtcga agcgctggat gtcgaacttg accgggccct
     4681 cggtcgaggc ggtcagcttg agccacacgt cgaccggcat cgccgggaag ctgtaacgcg
     4741 agggcggcgg gacggtggtg tacgccttga gctcgaagta gtcgctgtag acgtggatgt
     4801 aatagacgtc ggccgcgccg ccgccgttcc actgcacgac cggcccggtg agcccgcgcg
     4861 ggaacacggt gttgtcgtag gggtagacga gctgcatcgc cgggtcgggc agggtggcga
     4921 agccgaagcc gtccttgacg ccggggtcga ccggcatcgg ctcgctcgag aactgcagct
     4981 tgacgctcgc ggacgtcgag accggcccga gcgggccggc ggggtcgaag gtgacggtgc
     5041 ccttgccgcc ggcgaagccg gtgaccgcga actgcccgga ctgcgcgccg aggctggcga
     5101 ggtcgaggcg gtcgtaggcc caggtgccgg tgatcgggac ctcgaggccg ccctcggtga
     5161 tgcccttggc ggtatattgc atggcgccgg ggatctggcc gtcgacgacc gtgacgatcg
     5221 cgtcgagcgg gtcgatctgc acctcgacga tcgggtcgtc gccggtggtc gtgtcgtcgc
     5281 tcgtcatcgt cgtcgacacg gtggtcgggt cggtggccgt ggtgtcaccg gtgacctcgg
     5341 tcgccgagcc ctcgctcgcg gtgcccggcg tgccgccgct cgggtccgag ggctgcgagg
     5401 tcggcgtcgc cgtcgtcggc gtgtcgtcgg tgtcgccgct ggtcgtaccg ctgatcgaca
     5461 cgccggcggt gccgctgtcg gtgttgttgt cggtcccact gtcgcgaccg ccatcactac
     5521 atgccgtcgc gaacacacac acaccggtga ggagcagaga aagtctggtt gtcatggcca
     5581 ccctcgcgta aaactggaag cgggccccag tagagccgag tcgtcgcggg ctgaccagca
     5641 tggatctgtc ggcgaaacgt gcgctgtcac aggtaggagc cgacgcgctg cgcgagcatg
     5701 aacgcgggcg ggcgagacgc cgcgcgtgtc cgcgcgggat gtccttttgg acatgtccga
     5761 tgagaccggc gcggccttca gggcgcgagg atggccgcgg cggaggcctc ggcctcgagc
     5821 cggccgatcg tccgcgcgag ggtctcgcgc aggcaatcca ggcagtcctg cgagccgaag
     5881 aagccgcagc cctttcgccg ggtgtgcgcc aggcgcttga gcgcaggcag cacgcgcggg
     5941 tcggcggcga tctccatctt ctcgagcacg ccgcgcttgt tgccgcagct gctcgccttc
     6001 tcgagccagg cgaggttgag cgcgtaggcc ggcaccgcct ccttgggctt gtgctcgagc
     6061 acggcctcgg cggcgcgctt gcgggcgcgc ttctcctcgc cgtcgagcag ggtcgccagc
     6121 ggctgcgcga gcgtcggcgg cagcggcggc tcatcgccga cgggcgcagg gacggcgggc
     6181 gggggctgtg ggggcttcgc gtcggccctg gccggcggct tcgcggtggt gtccggggtg
     6241 gtgaccgggg tggcagccgg ggtggggtcg ggcggctcgg cgccctcggc ggcgaccgac
     6301 gcgacctcgt cgtcgagcac gcggtcttca ccatcgtcgg agaacagcag accgcaggtg
     6361 agcagcagcg cgaggccgcc ggcgatcgcc ggcagggcgc cgcggcgggc gcggacgtcg
     6421 tggaaggcgt cgagcagctt cgtgcgacgc gagcgcggcg gagcgagcac gtccggcacg
     6481 gcggcgcgc
//
LOCUS       DASWNJ010000729        10606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_506726, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000729 DASWNJ010000000
VERSION     DASWNJ010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 10606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10606
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506726"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1854
                     /locus_tag="VGB85_12165"
     CDS             <1..1854
                     /locus_tag="VGB85_12165"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY0134835.1"
                     /translation="NELMERAGDQVRSWFGGDDDGQQGGPSDGRGGPNQDNRSRNFGQ
                     DGDESERVRNNRDHNVGYSGQGYSGGMENYGHGSQGNYQGGSQGGSYSSGQGSHGGYG
                     GQSHQSNYGGQGSQYGGQSSFGSQSGAGYGSQGGYGQGGSQSYGGQGNFGAGGGQGGS
                     GQGGHGAQGGFGQGGSGQGNFGSQGGGYSGAGSQGGYGSQSGSQGNFSSQGGYGGYGN
                     IGGGQGGRGSQGGSQYGQGNFGSQGGHGGGSHGHGGHGGYGGGQQGGSQYGGGSQGGH
                     GGYGGYGNIGGGQQSGGQSGRGSQGGSQYGGGSQGGSQYGSGSQGGGSYGGSQYGGGS
                     QGGSQYGQGSQGGQYGGSQYGQGSQGGRSSQGGQGGFGQQSTQGDSGSNRGGYGQSQS
                     QDRGRHFGRGPKNYKRSDERLTEDVNERLTHHPDIDASEIDVSVQDGQVTLTGTVDDR
                     QTKRMIEDVVEEIHGVREVVNNLRVNRGSQGSQGSQGSQGSQSQGSQGGSQDFSGSST
                     GQRGGQSQQGSHSSSSGGSSVGTTSGGSSGTGGTGTTSGTGTSGTSSGTAAGTSGTSS
                     GTATGSGNTSAGGGAGSGNSGTPSSGPGSMGTGSSSDTSTDKGRGSNKSAT"
     gene            complement(2027..2659)
                     /locus_tag="VGB85_12170"
     CDS             complement(2027..2659)
                     /locus_tag="VGB85_12170"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HEY0134836.1"
                     /translation="MKERRTVAGTRLIWRRVRMVPLRTLAAMGTVTGMTARADRAISA
                     VTVGCLFEHVSEVMPCEVAIVPGAYVFPDGTPSDMLADRLMAALALYTAGKVTRVLVS
                     GGPDEVHGMQAWLAQRGVTAVISDPLGLRTWRTMQRAAALGVTRAVVCTQRFHLPRSL
                     FLAHAAGIAAVGLVADAGHYRGGAYNTSREACARLRALLDVRRARAKPQE"
     gene            complement(2656..3753)
                     /locus_tag="VGB85_12175"
     CDS             complement(2656..3753)
                     /locus_tag="VGB85_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134837.1"
                     /translation="MRIATLSIAAALLLGGCTSEIAFTDSVSSKYLQATPRAEFGDEL
                     STPYVLGSEFTVHVEDRQRDENMVGWTVRVTDPAVLTLRDPEPRWLDKHTLALDVKAS
                     GVGSAELLLVDASGEIKGSAAIEVRMPTRVKLYATAIAALENVDFRGETPHPRVLTGG
                     TASFAVQYLADDLLLQGSTDLKLAHEPTIVAEVVHSQLADNRDILQFTVGDPGRDTIG
                     LWLGEWSLGTVDVEVVGPSEVAAIDLIEHKGSVHADEEGDWLVVAQAYNEMRQPIYGV
                     GFEWNFPGRSFEQVGDILIYEYDPDPSKRKDVVARAAGREATISLPAADGEVYTSNDE
                     AFNCSVGDGGGAPLWAMVLLACAGLRRRRHR"
     gene            3947..5551
                     /locus_tag="VGB85_12180"
     CDS             3947..5551
                     /locus_tag="VGB85_12180"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0134838.1"
                     /translation="MDTPHSSPLTLAPPLTERSTIRCTDPATGELLGDVPAMDRAEVL
                     ARLGRARVAQASWKETTFAQRRRVVRRILDHVIDHSDELCRMISRDAGKTLENATIEL
                     WPVCEAMRWNLAHAEKALRPQKVSSGLLIHKSARIEYHPLGVIGVICPWNFPLQNVLA
                     PAIPAIMAGNAALVKVSEWTSWSAARIQQIFDEALRAEGHNIDLVQLLTGYAETGAAL
                     VSSGVDKIIFTGSMANGKRVLQASAETLTPVILELGGKDPMIVCDDADLEQAVHNALV
                     GTLTAAGQLCMAAERIYVFDAVYDDFVAMAARIVGQLRQGSPLGAIKVDVGAMTMPGQ
                     IDIVERLVDDAVARGARALVGGKRGEGGQYFQPTLLVDVDHSMAIMREETFGPVMTVM
                     RVRDEHEAIALANDTAYGLSSSVFTRDHARGERIASQIVAGSTVINDFAMHYMAQDLP
                     FGGVRGSGFGRLNGPEGLRAVCNVKAVVSDRLPFHNALKMYPVNPGAYDTARGVFKVL
                     YSGTLAGRVRGLVDVGRALLRKRPKD"
     gene            5597..5812
                     /locus_tag="VGB85_12185"
     CDS             5597..5812
                     /locus_tag="VGB85_12185"
                     /inference="COORDINATES: protein motif:HMM:NF012392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0134839.1"
                     /translation="MAAEDLDLALVQAHLRDPKLSISDVADRVGVHEAVFSKQFALQV
                     GMSPEDWRAQHCLPPESADEASDTDAA"
     gene            5881..7008
                     /locus_tag="VGB85_12190"
     CDS             5881..7008
                     /locus_tag="VGB85_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134840.1"
                     /translation="MHPGTDASPTPATDSPAVHASSSTPAAAIASAPVIAIATREDIA
                     RAPADMASLSRGRAWLALVVLAAGYAALRLEMNHEFEGAAWDELLAFTAAKPFGLRVL
                     VPLLAWPLHTLLRMPTTWVFGLFETLAAIALVLATGAALRPFVPPRWPTALGVALFLL
                     LPVPFLLQHRWPIFYPYDTPAMAFTAGGVALLVRGRWTLAAALTFVAALNRESAILLP
                     FAALALFFKNKPAPTDMPLATWPMRQLLPRVLAMFAAFALARGLVSAVLRDNPGAPLQ
                     FLVDGSPRLLQNFAWLEDPRHWLYLPAYFGLLPLAWLLLHAWIPAPLRRLGWVGLVYI
                     GALMWVANIDEPRVYGEAIVLLYIPVAVALVRWLRSGPTPV"
     gene            7022..7792
                     /locus_tag="VGB85_12195"
     CDS             7022..7792
                     /locus_tag="VGB85_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134841.1"
                     /translation="MIDVPPLSAAHKQLLFEEYQRRMRSCVPWSPAMPYTPEMNIIGD
                     TYHTLNVFPELAPSDAPVPQLNPAEHADSPNKQFRYCIRLHRAGHQLMTWFGDRTGSV
                     IFDSDVHIPAIYQCLPGQPGVWNETPWMSLTPAEILTLREGSRMARGRVVIAGLGLGH
                     QLIEVSKRLQVKEILLVELSQELVDFYLPRVRPLVKKRLTVLVGDAYQIVPTLTADVA
                     LIDIFPAYGGNEAATRELARRSPKIKKMWGWGTATLRR"
     gene            complement(7823..8422)
                     /locus_tag="VGB85_12200"
     CDS             complement(7823..8422)
                     /locus_tag="VGB85_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134842.1"
                     /translation="MQFRRSLLRLPVLALALSLVHCGGGKEGAVDDPVSAELSYIGLD
                     RAIDRAIDLGFDGFNAADSANIPEQTDAGELTGRMIVNGKVDQGASTNKNMSLNVTLV
                     DDYSDAVLEGEREVVYNGGPALLDLSFKGLPTATFTGSLHGSFAMTGDLAGEVTLDLQ
                     LTGETEAGAGDIIVRKPGTIRVIGTASSDYGVFDVDVSL"
     gene            complement(8522..8899)
                     /locus_tag="VGB85_12205"
     CDS             complement(8522..8899)
                     /locus_tag="VGB85_12205"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY0134843.1"
                     /translation="MSREYWGGRIVESDWQPGSSVFRRADGELDIVRAQVIEVEPERQ
                     LKMLWTYAKDPTPPASRVTYTIDRAGPEDLELTVIHEEFEPGSTVDEGLHNGWPAILS
                     SLKSFLETGRPLEVTKRWAREGR"
     gene            8975..10306
                     /locus_tag="VGB85_12210"
     CDS             8975..10306
                     /locus_tag="VGB85_12210"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0134844.1"
                     /translation="MSRLALVVAALAACTPATTSAPPGEPEPLCPEPTRPAKITADPS
                     AQIATVTTAAPPLLLRNARVIDGNGGPPIANTDVLIVAGRISRVGPKLAAPPSPGRTL
                     DLAGRTLLPGLIDTHVHMASDPQASHAEGIARDVASTDADAALRGAANARATLLAGFT
                     TVRNVGGGLADRSLRDAIAGGLVPGPRMLVANHSIGITGGHCDDTNGLRPDLFGGPPD
                     FTVGIADGPDELRKAVRHQIKLGADVIKICATGGVLSQGDAVGAPQLTLPEMQAVVDE
                     AVRAGRKVAAHAHGTVGIKEAVLAGVHSIEHGSVLDSETIALMKRRGTYLVPTVSVGE
                     YVEHAAASGKLSPESAAKAREIAPRMRESVVKAHKAGVKLAFGTDADAIVAATRDAAD
                     LLGLVSLGRIEVGYIADLVVVEGDPLADITALGRPSLVIQGGQIVRPPTWP"
     gene            10373..>10606
                     /locus_tag="VGB85_12215"
     CDS             10373..>10606
                     /locus_tag="VGB85_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134845.1"
                     /translation="MQIPRDLGLRLEAVDAPVLDPAPFRHAAVLAALLPGETPDASAR
                     LLLIERPGTMRAHAGQLAFPGGKPEPADRDLLDT"
BASE COUNT         1569 a         4115 c         3477 g         1445 t
ORIGIN      
        1 aacgagctga tggagcgagc cggcgatcag gtgcgctcgt ggttcggcgg cgatgatgac
       61 ggtcagcaag gtgggccgag cgacggccgc ggtggcccca accaggacaa ccgctcgcgt
      121 aacttcggcc aggacggcga cgagagcgag cgcgtacgca acaaccgcga ccacaacgtc
      181 ggctacagcg gccagggcta cagcggcggc atggagaact acggccacgg gagccagggc
      241 aactatcagg gcggctcgca gggcggcagc tacagcagcg gccagggcag ccacggcggc
      301 tacggcggcc agagccacca gagcaactac ggcggccagg gcagccagta cggcggccag
      361 agcagcttcg gctcgcagag cggcgccggc tacggctcgc agggcggtta cggacaaggc
      421 ggcagccaga gttacggcgg ccagggcaac ttcggagccg gcggcggcca ggggggcagc
      481 ggccaggggg gccacggcgc acagggtggc tttggtcagg gtggcagtgg ccagggtaac
      541 ttcggttccc agggcggtgg ctacagcggc gccggctcgc aaggcggcta cggctcgcag
      601 agcggcagtc agggcaactt cagctcccag ggcggctatg gcggctatgg caacatcggc
      661 ggcggccagg gtggtcgcgg ctcgcagggc ggcagccagt acggtcaggg caacttcggt
      721 tcccagggcg gtcacggcgg cggcagccac ggtcacggcg gtcacggcgg ctacggcggc
      781 ggccagcagg gcggcagcca gtacggcggc ggctcccagg gcggtcacgg cggctacggc
      841 ggctacggca atatcggcgg cggccagcag agcggcggtc agtccgggcg cggctcgcag
      901 ggcggcagcc agtacggcgg cggctcccag ggcggcagcc agtacggcag cggctcacag
      961 ggcggcggtt cgtacggcgg cagccagtac ggcggcggct cgcaaggcgg cagccagtac
     1021 ggccagggct cccagggcgg tcagtacggc ggcagtcagt acggccaggg ctcgcagggc
     1081 ggccgcagct cgcagggtgg tcagggcggc ttcggccagc agagcactca gggcgactcc
     1141 ggcagcaacc gcggcggcta cggccagagc cagagccagg atcgtggtcg tcacttcggg
     1201 cgcggtccga agaactacaa gcgctccgac gagcgcctga ccgaggacgt caacgagagg
     1261 ctgacgcacc acccggacat cgacgccagc gagatcgacg tctcggtgca ggacggccag
     1321 gtcacgctca ccggcaccgt cgacgatcgt cagaccaagc gcatgatcga ggacgtggtc
     1381 gaggagatcc acggcgtccg cgaggtcgtc aacaacctcc gcgtcaaccg cggctcgcag
     1441 ggctcgcagg gctcgcaggg ctcgcagggc tcgcagtccc agggctcgca ggggggctcg
     1501 caggattttt ccgggagctc gactggccag cgtgggggcc agtcgcagca gggcagccac
     1561 agcagcagct cgggtggcag cagcgtgggc accacgagtg gcggcagcag cgggaccggt
     1621 gggaccggca cgaccagtgg cacgggcacg agcggcacca gctcgggcac ggcggcgggc
     1681 acgagcggca ccagctcggg cacggcgacg ggctcgggca acacgagcgc gggcggcggc
     1741 gcgggttcgg ggaacagcgg cacgccgagc tcgggcccgg gcagcatggg aacgggcagc
     1801 tccagcgaca ccagcaccga caagggccgg ggatcgaaca agtcggccac ctgatcggcg
     1861 cgatcgcagg gacgctggcg gcgaggttcg tccagcgtct cgaccacctc ctcggcaagt
     1921 accgccagga gccatgaacg ccccccgcgg gtgacagagg cctcatcgca gcgcggtggg
     1981 gccgcttcgc gttggttcac cgccgcgaac cgccgcggac cgaccatcac tcctgcggct
     2041 tggcccgcgc ccgccgcaca tcgagcagcg ctcgcaagcg cgcgcacgcc tcacggctcg
     2101 tattataggc cccgccgcgg tagtgcccgg cgtcggcgac cagcccgacc gccgcgatgc
     2161 cggccgcatg cgcgaggaac agcgagcgcg gcaggtgaaa ccgctgggtg cacacgaccg
     2221 cccgcgtcac gccgagcgcc gcggcccgct gcatcgtccg ccacgtacgc agcccgagcg
     2281 ggtcggagat cacggccgtc acgccgcgct gcgccagcca ggcctgcatc ccgtgcacct
     2341 cgtcgggccc gcccgagacc agcacgcgcg tcaccttacc ggccgtgtac aacgccagcg
     2401 ccgccatcaa gcggtcggcg agcatgtcgg agggcgtgcc gtcggggaac acgtacgccc
     2461 cgggcacgat cgcgacctcg caaggcatga cctccgagac atgttcgaac agacagccga
     2521 ccgtcaccgc cgagatcgca cggtcggcgc gcgcggtcat cccggtcacc gtgcccatcg
     2581 ccgccagcgt gcgcagcggc accatccgga cgcgccgcca gatcaggcgc gtcccggcga
     2641 cggtcctgcg ctccttcacc gatggcgccg gcgtcgcagg ccggcacacg cgagcagcac
     2701 catcgcccac agcggcgcgc cgccgccgtc gccgacgcta cagttgaagg cctcgtcgtt
     2761 cgacgtgtag acctcgccgt cggccgccgg cagcgagatc gtcgcctcgc gacccgcggc
     2821 gcgcgcgacc acgtccttgc gcttcgaggg atcggggtcg tactcgtaga tcaggatgtc
     2881 gccgacctgc tcgaagctgc ggccggggaa gttccactcg aagccgacgc cgtagatcgg
     2941 ctggcgcatc tcgttataag cctgcgcgac gaccagccag tcgccctcct cgtccgcgtg
     3001 caccgagccc ttgtgctcga tgaggtcgat cgccgcgacc tcggacggtc cgacgacctc
     3061 gacgtcgacg gtgcccagcg accactcgcc gagccacagg ccgatcgtgt cgcgcccggg
     3121 gtcgccgacg gtgaactgca ggatgtcgcg gttgtccgcc agctgcgagt gcacgacctc
     3181 cgcgacgatc gtcggctcgt gcgccagctt gaggtccgtc gagccctgca gcagcaggtc
     3241 gtccgccagg tactgcaccg cgaacgaggc ggtgccgccg gtcagcacgc gcgggtgcgg
     3301 cgtctcgccg cggaagtcga cgttctcgag cgccgcgatc gccgtcgcgt agagcttcac
     3361 gcgcgtcggc atgcgcactt cgatcgccgc gctgcccttg atctcgccgc tcgcgtcgac
     3421 gagcagcagc tcggcgctgc cgaccccgct cgccttcacg tcgagcgcca gcgtgtgctt
     3481 gtcgagccag cgcggctccg ggtcgcgcag cgtcagcacc gccgggtcgg tgacgcggac
     3541 cgtccagccg accatgttct cgtcgcgctg gcggtcctcg acgtggaccg tgaactcgct
     3601 gccgagcaca tagggcgtcg acagctcgtc gccgaactcc gcccgcggcg tggcctgcaa
     3661 gtatttgctg ctgaccgagt cggtgaacgc gatctcactg gtacacccgc cgagcagcaa
     3721 tgccgcggcg atcgaaagcg tggcgatccg catgcattca ccctacgccc gcgccgtggc
     3781 agactcaagg gcgcccgcga ccggcttgcc gcatgaccgg cgccctccag cgactggagc
     3841 gtcatggccg ccggtctgcg cgatcgcgcc gtgggtccgg tcacctggcc ggggaccggg
     3901 ccccccggcg aacgtcggtc caaactgcgg tagggtgcgc gccgccatgg acacgccgca
     3961 cagctcgccc ctcacgctcg ccccgcccct gaccgagcgc tcgacgatcc gctgcaccga
     4021 ccccgcgact ggcgagctcc tcggcgatgt cccggcgatg gaccgggcgg aggtcctggc
     4081 ccgcctcggg cgcgcccgcg tcgcccaggc gtcctggaaa gagaccacct tcgcccagcg
     4141 ccgccgcgtc gtccggcgga tcctcgacca cgtgatcgat cacagcgacg agctttgtcg
     4201 catgatctcg cgcgacgccg gcaagaccct cgagaacgcc acgatcgagc tgtggccggt
     4261 gtgcgaggcg atgcgctgga acctcgccca cgccgagaag gccctgcgcc cgcaaaaagt
     4321 gtcctcgggc ctgctcatcc acaagtccgc gcgcatcgag taccacccgc tcggcgtcat
     4381 cggcgtcatt tgtccgtgga acttcccgct acagaacgtg ctcgcccccg cgatccccgc
     4441 gatcatggcc ggcaacgccg cgctggtgaa ggtcagcgag tggacctcgt ggtcggccgc
     4501 ccgcatccag cagatcttcg acgaggccct gcgcgccgag ggtcacaaca tcgacctcgt
     4561 gcagctgctg accggctacg ccgagaccgg cgccgcgctc gtctcctccg gcgtcgacaa
     4621 gatcatcttc accggctcga tggcgaacgg caagcgcgtc ctccaggcca gcgccgagac
     4681 cctcaccccc gtcatcctcg agctcggcgg caaggacccg atgatcgtgt gtgacgacgc
     4741 cgacctcgag caggccgtcc acaacgcgct cgtcggcacc ctgaccgccg ccggccagct
     4801 gtgcatggcc gccgagcgca tctacgtgtt cgacgccgtc tacgacgact tcgtggcgat
     4861 ggccgcccgc atcgtcggcc agctgcgcca gggctcgccg ctcggcgcca tcaaggtcga
     4921 cgtcggcgcg atgaccatgc ccggccagat cgacatcgtc gagcggctcg tcgacgacgc
     4981 cgtcgctcgc ggcgcgcggg cgctcgtcgg cggcaagcgt ggcgagggcg gccagtactt
     5041 ccagccgacg ctgctggtcg acgtcgacca ctcgatggcg atcatgcgcg aggagacctt
     5101 tggccccgtc atgaccgtca tgcgcgtgcg cgacgagcac gaggcgatcg cgctcgccaa
     5161 cgacaccgcc tacggcctca gctcctcggt gttcacccgc gaccacgccc gcggcgagcg
     5221 catcgccagc cagatcgtcg ccggctcgac cgtcatcaac gacttcgcca tgcactacat
     5281 ggcccaggac ctgcccttcg gcggcgtgcg gggctcgggc ttcggccgcc tcaacggccc
     5341 cgagggcctg cgcgccgtct gcaacgtcaa ggccgtcgtc tccgaccgcc tgccgttcca
     5401 caacgccctc aagatgtacc ccgtcaaccc cggcgcctac gacaccgctc gcggcgtgtt
     5461 caaggtgctg tacagcggca ccctcgccgg ccgcgtccgc ggcctcgtcg acgtcggccg
     5521 cgccctcctg cgcaagcgcc cgaaagactg acgggtcgca ctggcctgtg cgcccgaacc
     5581 ggcgcataag ccagccatgg ctgccgaaga cctcgacctc gccctcgtcc aggcccacct
     5641 ccgcgacccc aagctgtcga tctccgacgt cgccgaccgc gtcggcgtcc acgaggccgt
     5701 gttcagcaag cagttcgccc tccaggtcgg catgagcccg gaggactggc gcgcacagca
     5761 ctgcctgccg cccgagagcg ccgacgaggc cagcgacacc gacgccgcct gacctgccct
     5821 ggccaacccg acgacccgct tcccccgcgc ggccgccgtc gttcttgcat ataacgaggc
     5881 atgcaccccg ggaccgacgc gagccccacc cccgcgacgg acagcccggc cgtgcacgcg
     5941 agctcgtcga ccccggcagc ggcgatcgcg tcggcgcccg tgatcgccat tgcgacgcgc
     6001 gaggacatcg cccgggcgcc cgcggacatg gcctccctct cgcgcggccg ggcctggctc
     6061 gcgctcgtcg tcctcgccgc cggctacgcg gcgctgcgcc tcgagatgaa ccacgagttc
     6121 gagggtgcgg cctgggacga gctgctcgcc ttcaccgccg ccaagccctt cggcctgcgc
     6181 gtgctcgtcc ccttgctcgc gtggccgctg cacaccctgc tgcgcatgcc gacgacctgg
     6241 gtgttcggcc tgttcgagac cctcgctgcg atcgcgctgg tgctcgccac tggcgccgcg
     6301 ctgcgcccct tcgtcccgcc gcgctggccg accgccctcg gcgtcgcgct gttcctgctg
     6361 ctcccggtcc ccttcctgct gcagcaccgc tggccgatct tctaccccta cgacaccccc
     6421 gcgatggcct tcaccgccgg cggcgtcgcc ctgctcgtac gcggtcgctg gaccctcgcc
     6481 gccgcgctga ccttcgtcgc cgccctcaac cgcgagagcg cgatcctcct ccccttcgcc
     6541 gccctcgcgc tgttttttaa aaacaaacct gccccgacgg acatgcccct tgcgacatgg
     6601 ccgatgcgcc agctgctgcc gcgcgtgctc gccatgttcg ccgccttcgc gctcgcgcgc
     6661 ggcctcgtct cggcggtcct gcgcgacaac cccggcgcgc cgctgcagtt cctcgtcgac
     6721 ggctcgccgc gcctcctgca aaacttcgcc tggctcgagg acccgcgcca ctggctgtac
     6781 ctgcccgcct acttcggcct gctgccgctc gcctggctgc tgctgcacgc gtggatcccc
     6841 gcgccgctgc gccggctcgg ctgggtcggg ctcgtctata tcggcgccct gatgtgggtc
     6901 gccaacatcg acgagccccg cgtctacggc gaggcgatcg tgctgctcta catcccggtc
     6961 gccgtcgcgc tcgtccgctg gctgcgaagc gggcccaccc cggtgtagga tcggtgctgc
     7021 catgatcgac gtcccgccgc tgtcggccgc gcacaagcag ctcctcttcg aggagtacca
     7081 gcggcgcatg cgaagctgcg tgccctggtc gcccgcgatg ccctacacgc cggagatgaa
     7141 catcatcggc gacacctacc acaccctgaa cgtgttcccc gagctcgccc cctccgacgc
     7201 cccggtgccg cagctcaacc cggccgagca cgccgactcg cccaacaaac agtttcgcta
     7261 ctgcatccgc ctgcaccgcg ctggccacca gctgatgaca tggttcggcg accgcaccgg
     7321 cagcgtcatt tttgatagtg acgtacatat cccggccatc taccaatgcc tcccgggtca
     7381 gcctggcgtg tggaacgaga ccccgtggat gtcgctgacc cccgccgaga tcctcaccct
     7441 gcgcgagggc tcacgcatgg ccaggggccg cgtcgtcatc gccggcctcg gcctcggcca
     7501 ccagctgatc gaggtcagca agcgcctcca ggtcaaggag atcttgctcg tcgagctcag
     7561 ccaggagctc gtcgacttct acctgccccg cgtgcgcccc ctcgtcaaaa aacgcctcac
     7621 cgtgctcgtc ggcgacgcct atcaaattgt ccccaccctc accgccgacg tcgccctcat
     7681 cgacatcttc cccgcctacg gcggcaacga agccgccacc cgcgagctcg cccgccgtag
     7741 cccgaagatc aaaaaaatgt ggggctgggg caccgcgacc ctgcggcgct gatgctcccc
     7801 cgtcgccttc gctcgcccgc gatcacaggc tgacgtcgac gtcgaacacg ccgtaatcgg
     7861 agctcgccgt accgatcacg cggatcgtgc caggcttgcg gacgatgatg tcgccggcgc
     7921 ccgcctccgt ctccccggtg agctggaggt cgagcgtcac ctcgccggcg aggtcgccgg
     7981 tcatcgcgaa gctcccgtgc agcgagcccg tgaaggtcgc ggtgggcagc cccttgaacg
     8041 acaggtcgag cagcgcaggg ccgccgttgt acacgacctc gcgctcgccc tcgagcacgg
     8101 cgtccgagta gtcgtcgacc agggtgacgt tgagcgacat gttcttgttg gtggacgcgc
     8161 cctgatcgac cttgccgttg acgatcattc ggcccgtgag ctcgccggcg tccgtctgct
     8221 ccgggatgtt ggcgctgtcg gccgcgttga agccgtcgaa gccgaggtcg atcgcgcggt
     8281 cgatcgcgcg gtcgaggccg atgtacgaca gctcggccga caccgggtcg tcgaccgccc
     8341 cctccttccc gcccccgcag tgaaccagcg agagcgcgag cgcgaggacc gggagacgaa
     8401 ggaggctgcg gcgaaactgc atccccgcac catacctcag gcccgccccc gcggcaggtc
     8461 cacgtgcgac cacggcgcgc cagcgagccc cggctccctt ctgcgaatgc tgctgccggc
     8521 gctaccggcc ctcgcgcgcc cagcgtttgg tcacctcgag cggccgcccg gtctcgagga
     8581 agctcttgag gctcgacagg atcgccggcc agccattatg tagaccctcg tcgaccgtgc
     8641 tgcccggctc gaattcctcg tggatcacag tcagctcgag gtcttccggc ccggcgcggt
     8701 cgatcgtgta cgtcacccgc gaggccggcg gcgtcgggtc cttcgcgtag gtccacagca
     8761 tcttgagctg tcgctccggc tcgacctcga tcacctgcgc gcgcacgatg tccagctcgc
     8821 cgtcggcgcg acgaaacacc gacgagcccg gctgccagtc ggactcgacg atccgcccgc
     8881 cccaatactc gcggctcagt gcctgtcaaa caccccacga attccggccc tcaccgccgt
     8941 ttacgggcgc ttgcgtgcca ggcatgctcg cgccatgtcc cgcctcgccc tcgtcgtcgc
     9001 cgcgctcgct gcatgtacac cggcgaccac ctcggccccg ccgggcgagc ctgagccgct
     9061 gtgtcccgag ccgacgcgcc cggccaagat caccgccgac ccctccgccc agatcgccac
     9121 cgtcaccacc gctgccccgc cgctgctgct ccgcaacgcg cgcgtgatcg acggcaacgg
     9181 cggcccaccg atcgccaaca ccgacgtcct gatcgtcgcc ggccgcatca gccgagtcgg
     9241 tccgaagctc gccgccccgc cgagccccgg ccgcaccctc gacctcgccg gtcgcacgct
     9301 gctccccggc ctgatcgaca cccacgtgca catggcctcc gacccgcagg ccagccacgc
     9361 cgagggcatc gcccgcgacg tcgcctcgac cgacgccgac gccgccctgc gcggggccgc
     9421 caacgcccgc gccaccttgc tcgccggctt caccaccgtg cgcaacgtcg gcggcggcct
     9481 ggccgaccgc agcctgcgtg acgcgatcgc cggcggcctc gtgcctggcc cgcgcatgct
     9541 cgtcgccaac cattcgatcg gcatcaccgg cggccactgc gacgacacca acggcctgcg
     9601 ccccgacctg ttcggcggac cacccgactt caccgtcggc atcgccgacg gccccgacga
     9661 gctgcgcaag gccgtccgcc accagatcaa gctcggcgcc gacgtcatca aaatctgcgc
     9721 caccggcggc gtgctctcgc agggcgacgc cgtcggcgcc ccgcagctga ccctccccga
     9781 gatgcaggcc gtcgtcgacg aggccgtgcg cgccggtcgc aaggtcgccg cgcacgcgca
     9841 cggcaccgtc ggcatcaagg aggccgtgct cgccggcgtg cacagcatcg agcacggcag
     9901 cgtgctcgac agcgagacga tcgccctgat gaagcgccgc ggcacctacc tcgtgccgac
     9961 cgtctccgta ggcgagtacg tcgagcacgc cgccgcctcc ggcaagctgt cccccgagtc
    10021 cgccgccaag gcgcgggaga tcgcgccgcg catgcgcgag agcgtcgtca aggcccacaa
    10081 ggccggcgtc aagctggcct tcggcaccga cgccgacgcg atcgtcgccg ccacgcgtga
    10141 cgccgccgac ctgctcggcc tcgtatcact cggccggatc gaggtcggct acatcgccga
    10201 cctcgtggtc gtcgagggcg acccgctcgc cgacatcacc gcgctcggcc gcccatctct
    10261 ggtgatccag ggcggccaga tcgtccgccc gccgacctgg ccgtgacccc gcgctcgtga
    10321 ccgccagcgc ccgcgcctgg cccgcgcgcg ccccttttgc catcatcccc gcgtgcagat
    10381 cccacgcgac ctcggccttc gcctcgaagc cgtcgacgct cccgtcctcg accccgcgcc
    10441 gtttcgccac gccgccgtgc tcgccgccct gctgcccggc gagacgcccg acgcgtccgc
    10501 ccgcctgctg ttgatcgagc gccccggcac catgcgcgcg cacgctggtc agctcgcctt
    10561 ccccggcggc aagcccgagc ccgccgaccg cgacctgctg gacacc
//
LOCUS       DASWNJ010000730         2606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_365803, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000730 DASWNJ010000000
VERSION     DASWNJ010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365803"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2425)
                     /locus_tag="VGB85_12220"
     CDS             complement(<1..2425)
                     /locus_tag="VGB85_12220"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY0134846.1"
                     /translation="MARTPPSRVGEPVAQPELPLPPAGLSDRSDEPSQQRGLPLPSVL
                     SDRSDEPGPKARSRPRVKSEAKVEAPARARASAKQVAPADGPASAWDGLAGLPGIGPK
                     TADKLRAQGIHDCWDLVRWIPTGYRDHRRRDRVDLVADGALAVIEATIRRFRQSFFRG
                     RYNAAMELVQDTPDGPRVFVARWFHRVGGLAERSAAGKRVRLIGKAQRKGETVALVHP
                     EVHDAEVEGAAIAVEYPDIEGLGDGSVARLCLAALARIEARAPADPLPDSLRERLGLP
                     ALLPALRMLHAPPESLPDAALREIEAGKSPAHRRVLFDDLLHVQLALLRQRAAYRAAA
                     APVGLLSGDVDRRERLRACVPFEPTGAQWRAVDEIETDLGSPRPMLRLVQGEVGAGKT
                     LVAFAAALGVIDAGGQAAIMAPTEILAAQHLQSLAPWCEAAGVRIGLLTGSTPRPQRA
                     GLLARAQAGTLDLLIGTHALLTEGVEFRRLGLVVIDEQHRFGVRQRALLRDKGSAPHL
                     LVMTATPIPRTLALTLFGELDVSVLDELPPGRTPPVTRLFAGPRSLASARTALAGLVE
                     RGRSAFVVCPQVEAGTREVSDVEEAAADLRVRLPGREIGVLHGRMAARDKEAAVAAFR
                     AGEIDVLVATTVIEVGVDIPAAQAMLVEHADCFGLAQLHQLRGRIGRGSPVLSDRPAR
                     GQLKLDAPAPPRPAPIRKVDPGPPVCLLHTANSPASEAAARLQVLVDTCDGFQVAERD
                     LAERGPGELFGLRQAGAPRLRIAGLAGEVTRLLEAARTAAREILARDPDLRAHPELRA
                     ELERRAIAGV"
BASE COUNT          336 a         1000 c          952 g          318 t
ORIGIN      
        1 cgacccctgc gatcgcgcga cgctcgagct cggcgcgtag ctccgggtgc gcgcgaagat
       61 cggggtcgcg cgccaggatc tcccgcgcgg cggtccgcgc ggcctccagc agccgcgtga
      121 cctcgccggc gagcccggcg atccgcagcc gcggcgcgcc ggcctggcgc agcccgaaca
      181 gctcgccagg cccgcgttcg gcgaggtcgc gctcggcgac ctggaagccg tcgcaggtgt
      241 cgacgagcac ctgcagccgc gccgccgcct ccgaggccgg gctgttcgcg gtatgcagca
      301 ggcacaccgg cggccccggg tcgaccttgc ggatcggggc ggggcggggc ggggcagggg
      361 cgtcgagctt gagctgcccg cgcgcgggcc tgtccgacag gacaggtgat ccgcgaccga
      421 tccgcccgcg cagctggtgc agctgggcga gcccgaagca gtcggcgtgc tcgacgagca
      481 tcgcctgcgc cgccgggatg tcgacgccga cctcgatcac cgtcgtcgcg accaatacgt
      541 cgatctcacc cgcgcgaaac gccgcgaccg cggcctcctt gtcgcgcgcg gccatgcggc
      601 cgtgcagcac cccgatctcg cggcccggca ggcgcacgcg caggtcggcg gccgcctcct
      661 cgacgtcgct cacttcgcgg gtgccggcct cgacctgcgg gcagaccacg aacgccgagc
      721 gcccgcgctc gaccagcccc gcgagggccg tgcgcgcgct ggcgaggctg cgcggccccg
      781 cgaacagccg cgtgaccggc ggcgtacgac cgggcggcaa ctcatcgagc accgacacgt
      841 cgagctcacc gaacagcgtc agcgcgagcg tgcgagggat cggcgtcgcc gtcatcacca
      901 gcaggtgcgg cgcgctgccc ttgtcgcgca gcagggcccg ctggcgcacg ccgaagcggt
      961 gctgctcgtc gatcaccacc aggccgaggc ggcgaaactc gaccccctcg gtcagcagcg
     1021 cgtgcgtgcc gatcagcagg tcgagcgtgc ccgcctgcgc gcgcgccagc aggcccgcgc
     1081 gttgcggccg cggcgtcgag cctgtcaaca ggccgatccg cacccccgcc gcctcgcacc
     1141 acggggcgag gctctgcagg tgctgggccg cgaggatctc cgtcggggcc atgatcgccg
     1201 cctgaccgcc cgcgtcgatc accccgagcg ccgccgcgaa ggcgacgagc gtcttgccgg
     1261 cgccgacttc cccctggacc agccgcagca tcggtcgcgg gctgccgagg tccgtctcga
     1321 tctcgtcgac cgcgcgccac tgcgcccccg tcggctcgaa cggcacgcag gcccgcagcc
     1381 gctcgcggcg gtcgacatca ccgctgagca gcccgaccgg cgccgccgcg gcccggtaag
     1441 ccgcgcgctg gcgcagcagc gcgagctgta cgtgcaacag gtcatcgaac agcacgcgcc
     1501 ggtgcgccgg cgacttgccg gcctcgatct cgcgcagcgc cgcgtccggc agcgactcgg
     1561 gcggcgcgtg cagcatcctc agcgcgggca gcagggccgg cagaccgagc cgctcgcgca
     1621 gcgagtcggg cagggggtcg gcgggcgcgc gcgcctcgat gcgcgcgagc gcggcgaggc
     1681 acaggcgcgc gacgctgccg tcgccgagcc cctcgatgtc ggggtactcg acggcgatcg
     1741 ctgcgccctc gacctccgcg tcgtgcacct ccgggtgcac gagcgcgacc gtctcgccct
     1801 tgcgctgggc cttgccgatc aggcgcacgc gcttgccggc cgccgaccgc tccgcgagcc
     1861 cgccgacgcg atgaaaccac cgcgcgacga acacccgcgg cccgtccggc gtgtcctgga
     1921 ccagctccat cgccgcgttg tagcgcccgc gaaagaagct ctggcggaac ctgcgtatgg
     1981 tcgcctcgat caccgcgagc gccccgtcgg cgacgaggtc gacgcgatcg cggcggcgat
     2041 gatcgcggta gcccgtcggg atccagcgca ccagatccca gcagtcgtgg atcccctgcg
     2101 cgcgcagctt gtcggccgtc ttcggcccga tcccgggcag cccggcgagc ccgtcccacg
     2161 cgctcgccgg gccgtccgcg ggcgcgacct gcttcgcgga ggcgcgcgca cgggcagggg
     2221 cctcgacctt cgcctcgctc ttgacgcgcg gccgcgacct ggccttcggg ccaggttcgt
     2281 cggacctgtc tgaaaggaca gacggcagcg gtagcccgcg ctgctgcgag ggctcgtcgg
     2341 acctgtcgga gaggccagct ggcgggagcg gcagctccgg ctgtgcgacg ggctcgccga
     2401 cccggctcgg gggagtgcgg gccacggggc tcgtgactac gtgacgctgc ggggcgtcag
     2461 cccatcgtgc tgggcggcgt gaacgagaag cgcagctcgt ggtcgcagag gaagtacacg
     2521 ctgccctcga cgatcttgtg gttgtcgacg cgcgtgccct cgaactcgac gccgttggtg
     2581 ctgccgagat ccttgatgaa gtaatc
//
LOCUS       DASWNJ010000731         3383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_365857, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000731 DASWNJ010000000
VERSION     DASWNJ010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365857"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VGB85_12225"
     CDS             <1..612
                     /locus_tag="VGB85_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134847.1"
                     /translation="DLGLDNLEADAPDVRQSAAPPPAPQRADSGPHGHPASGPYGHPA
                     SGPHRADSGPQAAGPRWEGPQHVPPPPPPIPGTGPNRTLSVRSVPPPPPREVKGRGRG
                     KPPPAPGSGPAKAIVVTKKTGSNATVGGREKTGSNEAVPAGDKRGAKRKKVVDLSQPV
                     VRPGSGPMVGAAPLAERPLPADDEVLPNPRHTAVPDYLRDDDE"
     gene            complement(668..2113)
                     /locus_tag="VGB85_12230"
     CDS             complement(668..2113)
                     /locus_tag="VGB85_12230"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HEY0134848.1"
                     /translation="MSSTPTHSASSEPLRPEDREPLLELFRRAERPEQRSHLVGTEQE
                     KFGLVLDPAQPHMNPRPVEYEAHVLPVLTAFIEEFGWKPAADRGPGGEIIALERDGAS
                     ITLEPGGQFELSGKPLPHVHATCAEFTQHYHELHGVSQRLGLAWLATGFHPFATREEI
                     HWMPKGRYRVMRDFLPTRGTRGIDMMLRTCTVQANFDFASEQQAGERFRVAMGISGLS
                     TALFANSPYREGEDRGFASLRSENWTDVDNARCGMLPLAFEPGFSYERYAEWALDVPM
                     FFVRREGQYIPCHVPFRVYMRDGFTAPDGVHHRALRGDWELHLSTLFPEIRLKPYLEV
                     RGCDSVSSKFLCALPALWKGILYDEDATQAAWELVADLDFPARQALWHECREHAMRSP
                     RVQQLCARLLAIARTGLDRQDVRDSKGRTEARFLDGLEALVQSGKSPADLAREALGKS
                     PGRGAAARLAFARHFHFAGAGLDEVTADPDA"
     gene            2340..2705
                     /locus_tag="VGB85_12235"
     CDS             2340..2705
                     /locus_tag="VGB85_12235"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY0134849.1"
                     /translation="MSNPAGAPCNIGPRIAIRGNVSGEEDLVIEGRVEGSIALTGHLT
                     IAAGAVVEADLEVDSVDVHGQLDGDIVAATTITLHEGCRVAGNLRAPRVVLTDGAQFR
                     GAVEMDVQLPGSVQRQQRR"
     gene            2722..3036
                     /locus_tag="VGB85_12240"
     CDS             2722..3036
                     /locus_tag="VGB85_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY0134850.1"
                     /translation="MATTVIGSSIVIDGEISGEEQLVVHGTIKGRISVRDSLVVENGG
                     VVEATVESASITINGTVNGDISASERAELRPNCTVVGDIRAPRILIADGASFKGSVDM
                     GG"
     gene            3094..>3383
                     /locus_tag="VGB85_12245"
     CDS             3094..>3383
                     /locus_tag="VGB85_12245"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY0134851.1"
                     /translation="MATSIGAGTRVAGRLSGQEDVRVHGTIEGSVRLAQTLFIEPEGV
                     ALAEIYARDVVVSGTVVGNIHAENAVVLAATARVVGDIRSPRVSIEPGAAFRG"
BASE COUNT          510 a         1275 c         1166 g          432 t
ORIGIN      
        1 gatctcggcc tcgacaacct cgaggccgac gcccccgacg tccgccagtc cgccgcgccc
       61 ccgcccgctc cgcagcgcgc cgattcgggc ccgcacgggc acccggcctc gggtccgtac
      121 gggcacccgg cctcgggccc gcatcgcgcc gactcgggcc cgcaggccgc gggtccgcgc
      181 tgggagggcc cgcagcacgt cccgccgcca cccccaccga tcccggggac cgggcccaac
      241 aggaccctgt cggtgcgcag cgtcccgccg ccgccaccac gcgaggtaaa gggccgtggc
      301 cgcggcaagc ccccgcccgc acccgggtcg ggcccggcca aggcgatcgt cgtcaccaag
      361 aagaccggct cgaacgcgac tgtcggcggc cgcgagaaga ccggctcgaa cgaggcggtc
      421 ccggcgggag acaagcgcgg cgccaagcgc aagaaggtcg tcgatctcag ccagccggtc
      481 gtgcgcccgg gctcggggcc gatggtcggc gcagcgccgc tcgccgagcg gccgctcccc
      541 gccgacgacg aggtgctccc caacccgcgc cacaccgccg tgccggacta cctgcgcgac
      601 gatgacgagt agcgagtagc gagtagcgag cagcgagccg ccgcggagac ctgtccccgc
      661 ggacagctca ggcgtccggg tcggcggtga cctcgtcgag ccccgcgccg gcgaagtgga
      721 agtgccgcgc gaacgccagg cgggccgccg cgccgcggcc tggcgacttg ccgagggcct
      781 cgcgggcgag gtcggccggc gacttgccgg actgcaccag cgcctcgagc ccgtcgagga
      841 agcgggcctc ggtgcggccc ttgctgtcgc ggacgtcctg ccgatcgagc ccggtgcgcg
      901 cgatcgccag cagcctcgcg cacagctgtt gcacccgcgg cgagcgcatc gcgtgctcgc
      961 ggcactcgtg ccacagcgcc tggcgggccg ggaagtcgag gtccgcgacg agctcccacg
     1021 ccgcctgcgt cgcgtcctcg tcgtagagga tgcccttcca cagcgccggc agggcgcaca
     1081 ggaacttcga gctcaccgag tcgcagccgc ggacctcgag gtacggcttg agccggatct
     1141 ccgggaacag ggtgctcagg tgcagctccc agtcgccgcg cagggcccgg tggtgcacgc
     1201 cgtccggcgc cgtgaagccg tcgcgcatgt acacgcgaaa cggcacgtgg cacgggatgt
     1261 actggccctc gcggcggacg aagaacatcg gcacgtcgag cgcccactcc gcgtagcgct
     1321 cgtagctgaa gcccggctcg aaggccagcg gcagcatgcc gcagcgggcg ttgtcgacgt
     1381 cggtccagtt ctcgctgcgc agcgacgcga acccgcggtc ctcgccctcg cggtacggcg
     1441 agttggcgaa cagcgccgtc gacaacccgc tgatccccat cgccacccgg aagcgctcgc
     1501 cggcttgctg ctccgacgcg aagtcgaagt tcgcctgcac ggtgcacgtg cgcagcatca
     1561 tgtcgatgcc ccgcgtgccg cgggtcggca ggaagtcccg catcacgcgg tacctgccct
     1621 tgggcatcca gtggatctcc tcgcgcgtgg cgaaggggtg aaatccggtc gccagccagg
     1681 ccaggccgag ccgctgcgag accccgtgca gctcgtggta gtgctgggtg aactcggcgc
     1741 aggtcgcgtg cacgtggggc aggggcttgc cgctgagctc gaactgcccg cccggctcga
     1801 gggtgatcga cgccccgtcg cgctcgagcg cgatgatctc cccgccgggc ccgcggtcgg
     1861 ccgccggctt ccagccgaac tcctcgatga aggccgtcag caccgggagc acgtgggcct
     1921 cgtactcgac cggccgcggg ttcatgtggg gttgagctgg atccaacacc agcccgaact
     1981 tctcctgctc ggtgcccacc aggtgcgacc gctgctcggg gcgttcagcc cgccggaaca
     2041 gttccaggag cggctcgcga tcttcgggcc ggagcggctc ggacgaggcg gagtgggtcg
     2101 gggtgctgct cacgggggct ccagggctaa agcgggacga cgggatcgcc gatgaagatc
     2161 ggcgtcacgg cggccgagtg tagccgcagc cccggccgac ttgaccgacg ttcccgagaa
     2221 acttgcccgc cgcgcctgca agcctcgctt gacccggtcc ggcccgcacc gctaatctgc
     2281 gcccgcgtgc cgcgctcgcc gcgttgcgca ggaaaccaga tcgtacgatg gccacacccg
     2341 tgagcaaccc ggcaggcgcc ccctgtaaca tcggtccgcg gatcgccatc cgcggcaacg
     2401 tctccggcga ggaagacctc gtcatcgagg gccgcgtcga gggcagcatc gccttgaccg
     2461 gccacctgac gatcgccgcc ggcgcggtcg tcgaggccga cctcgaggtc gacagcgtcg
     2521 atgtccacgg ccaactcgac ggcgacatcg tcgccgccac cacgattact ctccacgaag
     2581 gctgccgcgt cgccggcaac ctccgcgcgc cgcgcgtggt cctcaccgat ggcgcccaat
     2641 tccgcggcgc cgtcgagatg gacgtccagc tgcccggcag cgtccagcgt cagcaacgcc
     2701 gctaagctcg aaggaacaat catggccacc accgtcatcg gcagcagcat cgtcatcgac
     2761 ggggagatct ccggcgagga gcaactcgtc gtgcatggca ccatcaaggg ccgcatcagc
     2821 gtgcgtgact ccctcgtcgt cgagaacggc ggggtcgtcg aggccaccgt cgagtcggcc
     2881 tcgatcacga tcaacggcac cgtcaacggc gacatctcgg ccagcgaacg cgccgagctg
     2941 cggccgaatt gcaccgtcgt cggggacatc cgcgcgccgc ggatcctgat cgccgatggc
     3001 gcgtcgttca agggcagcgt cgacatgggg ggctaacggg gcatggcaac gaagaccagg
     3061 aacaatctcc cttctccgga agccagcgcc gaggtggcca cctccatcgg cgccggcacg
     3121 cgcgtcgccg gtcgcctctc gggccaggag gacgtgcgcg tccacggcac gatcgagggc
     3181 agcgtccgcc tcgcccagac cctcttcatc gagcccgagg gcgtcgccct cgcggagatc
     3241 tacgcccgcg acgtcgtcgt ctccggcacg gtcgtcggca acatccacgc cgagaacgcc
     3301 gtcgtcctgg ccgccaccgc ccgcgtcgtc ggtgacatcc gttcgccgcg cgtcagcatc
     3361 gagcccggcg ccgccttccg cgg
//
LOCUS       DASWNJ010000732         1727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_49231, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000732 DASWNJ010000000
VERSION     DASWNJ010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49231"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /gene="sppA"
                     /locus_tag="VGB85_12250"
     CDS             <1..985
                     /gene="sppA"
                     /locus_tag="VGB85_12250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEY0134852.1"
                     /translation="TFNETDPEYPTWSTAPYLAAVVIEGTIIDGESRMIPFLGIQFTG
                     GDTIVKQLRTLRADPMCKGIILRVNSPGGSALASDVVWREVARTHEAFEKNPKKSPPI
                     VVSMGDVAASGGYYVAMGARTIFASSMTLTGSIGVVSLHFDLSGLFAKLGISTTTFQR
                     GKNADIASIYKPYTDDQRARMDASMRRVYDLFRGRVAEGRDMKIEDVDKLGRGHVYSG
                     TDAVALKLVDQLGGLHEALFAVRSKAGLPARRDIDLRIFPRQRRLVDIVLEQLIPQSE
                     KGLRTRIADRREARQQLPLALDAALSRLPLALLFLPQDQALALLPGLVEVE"
     gene            993..>1727
                     /locus_tag="VGB85_12255"
     CDS             993..>1727
                     /locus_tag="VGB85_12255"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0134853.1"
                     /translation="MVKLKRLKINQYRNVRPGTELHFDDGVNLVLGKNGAGKTTLLAL
                     LSHVAGSDFSSLQGESFNLEYDFSGGDFTARVSIWASAQSGAPDDPRYAERSNLHLPA
                     QQTFEYRILISLDGSAETSEITGTPEETCLIVGSERQEHRLAPLNPLQHGTSFLDTLF
                     HIDVESPFNRAWSHLYVPLAARFDESLDAFLAMTGRASVTASAGTPPIARLSVTHFTL
                     RNGHAWTRDFAPRAIGFLIPAAHERPA"
BASE COUNT          317 a          635 c          480 g          295 t
ORIGIN      
        1 caccttcaac gagaccgacc ccgagtaccc gacctggtcg accgcgccct acctggccgc
       61 cgtcgtcatc gagggcacga tcatcgacgg cgagagccgc atgatcccct tcctcggcat
      121 ccagttcacc ggcggcgaca cgatcgtcaa gcagctgcgg accctgcggg ccgacccgat
      181 gtgcaagggc atcatcttgc gcgtcaacag ccccggcggc tccgcgctcg cctcggacgt
      241 cgtctggcgc gaggtcgccc gcacccacga ggcctttgaa aagaacccga agaagagccc
      301 gccgatcgtc gtgtcgatgg gcgacgtcgc cgcctccggc ggctactacg tcgcgatggg
      361 cgcgcggacg atcttcgcct cgtcgatgac cctcaccgga tcgatcggcg tcgtcagcct
      421 gcacttcgac ctctcgggcc tgttcgccaa gctcggcatc tcgacgacca ccttccagcg
      481 cggcaagaac gccgacatcg ccagcatcta caagccgtac accgacgacc agcgcgcgcg
      541 catggacgcc tcgatgcgcc gcgtctacga cctgttccgc ggccgcgtcg ccgagggtcg
      601 cgacatgaag atcgaggacg tcgacaagct cggccgcggc cacgtctact ccggcaccga
      661 cgccgtggcg ctcaagcttg tggaccagct cggtgggctg cacgaggccc tgttcgccgt
      721 gcgcagcaag gccggcctgc ccgcgcgccg cgacatcgac ctgcgcatct tcccccgcca
      781 gcgccgcctc gtcgacatcg tcctcgagca gctgatcccg cagtccgaaa agggcctgcg
      841 cacccgcatc gccgaccgcc gcgaggcccg ccaacagctc cccctcgcgc tcgacgccgc
      901 cctctcgcgc ctcccgctcg ccctcctgtt cctcccacaa gaccaggccc tcgccctgct
      961 cccgggcctc gtcgaggtcg agtgagcccg ccatggtcaa gctcaagcgc ctgaagatca
     1021 accagtaccg caacgtgcgc cccggcacgg agctgcactt cgacgacggc gtgaacctgg
     1081 tgctgggaaa gaacggcgcc ggaaagacca ctctcctcgc gctcctgagc cacgtggcag
     1141 ggagcgactt ctcatcccta caaggcgagt cttttaatct cgaatacgat ttctccggcg
     1201 gtgacttcac cgcccgcgtc agcatttggg cgagcgctca gagcggtgcg ccagacgacc
     1261 ccaggtatgc cgagcgtagc aaccttcatt tgccggccca acaaaccttc gagtaccgca
     1321 tactaatatc gctcgacgga tccgcagaaa cctccgagat tacagggaca ccggaagaga
     1381 catgtttaat cgtcggcagc gaacgacaag agcatcgttt agcaccccta aaccctctac
     1441 aacacgggac ttcttttctc gacacgctat ttcatattga tgtcgagtcc ccatttaaca
     1501 gggcttggag tcacctttac gtccccctcg cggcgcgctt cgatgagtct ctggatgcgt
     1561 ttctcgccat gactggacga gcatccgtga cggcgagcgc tggaactcct cctatcgcgc
     1621 gattgtcggt tactcacttt acacttcgaa acgggcacgc atggacgcgc gattttgcgc
     1681 ccagagcaat cggatttcta attccagcgg cccacgagcg tcccgcg
//
LOCUS       DASWNJ010000733         6869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_296848, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000733 DASWNJ010000000
VERSION     DASWNJ010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6869
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296848"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VGB85_12260"
     CDS             <1..278
                     /locus_tag="VGB85_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134854.1"
                     /translation="DVLIVTDESGILVSSSTTELDLAMLAAVTPLVARGSAKANIKRD
                     GAMREYSVRSIKVLGETLYVGALGGKFGPRERELATTASAARRILLS"
     gene            complement(296..1510)
                     /locus_tag="VGB85_12265"
     CDS             complement(296..1510)
                     /locus_tag="VGB85_12265"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0134855.1"
                     /translation="MARMIQRGSGLAFALGALFMWAVQQGWPERQAEAGSHTLSRAGF
                     DAALRTVLERYVDPVESSPVLTETLKRIVSGLDTYSHFLTADERAEIRQRSRGGSTGM
                     ETLLHRAEPGARKPASLEISAVLPGSPAEAAGLHPGDQILKIRGQDVGFLLSQAEAAA
                     LLMGADGDTLEMSVQRRSDPAPIAVRLVLTGQPKNLVEGRVIDHDGRKFAHLRVRAFR
                     GGTAELVKRSLKDMRRAAGSTLAGLVLDLRGNPGGDVPEAVMIADMFVADGVLVRTRG
                     RGGQILREERAHPDGSDSALPLVVLQDQRSASASELLAVALQDHGRARVVGERSFGKG
                     TVQDVIGLHDGSLLTLTIARYFSPKDRLIDGNGVDPDLHVALPPVPPTQPDQPDVGLQ
                     AALADLARSTAG"
     gene            complement(1557..1760)
                     /locus_tag="VGB85_12270"
     CDS             complement(1557..1760)
                     /locus_tag="VGB85_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134856.1"
                     /translation="MHTSTVMLRNRPDAFIHSMVTAATVRPFWTTLMEMKQARLRIAR
                     LVSAPLPVPAPRETPVIATPQAA"
     gene            complement(2097..2525)
                     /locus_tag="VGB85_12275"
     CDS             complement(2097..2525)
                     /locus_tag="VGB85_12275"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0134857.1"
                     /translation="MRCVIMPRQASQRELIMNEVAQEIGRRIRLWRTATPPPKTRRKN
                     QGETGWRPHMTIEELAKQTGLTVTTVNKLELGYKAPRIESLLLLATAFGCDPTELLPR
                     AKQKGGAKAELLDELNAVAAQLSPKQIRELVKSARGMEPG"
     gene            complement(2632..5034)
                     /locus_tag="VGB85_12280"
     CDS             complement(2632..5034)
                     /locus_tag="VGB85_12280"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0134858.1"
                     /translation="MSGLAALVLAGLAVMAAAPTSGPVPEARSAPAFAAPVTGRARLS
                     GQVLVYGSRDVVFGARILGTTADIATATDAEGRFELWLPPGEHALTIRAAGFRELPIK
                     VKLVDGQDLVYEYRLAPDLDGNPYRTVVRQQREVAISSTNLRDDEIHALPGTFGDPFR
                     VVKSLPGASQLAGFLPYVVVRGAAPGNTGYFLDGVRVPILFHVAIGPSVIHPYFIDQV
                     DFYPGGAPVRLGRYVSGMIEGRTKAARRDRVFGEFDIRITDAGGIVEVPLNRRRDAAC
                     RRQKKHGKMDAKARAKLDCRRGEARGSLTLAGRYSYTAGVLTLVQSTAKINFWDYQAR
                     FDHKLGQRAQYTAFAYGSFDSLGDKLSPDPLVRFEFYRIQQRVRQSLPRGGTAVYSVA
                     LGLDRSGVTPSQVAASPIRTAEWQISPRIDVRLPLGERSNGEFGFGVDQEFQIFRSDT
                     NVSSPSVTDYLSVLLGDRFVSATGLYAELLWKKGPIEVRPGVRTDIYAQIGPSPVLPT
                     ATSVTHAIGVDPRILFREKINDRWTLRQNFGVYHQPPSFPVPIPGVESFGFERGLQRN
                     IQGSLGYEFSIADRAILTQDAYLGRLDNLQDYDLAAAAQGNISEFDDLVIRAKGLAYG
                     LETMLRLAPRQRVYGWAAYTLSRSTRTFENGTESASNWDQRHILNIVLGYRIGEKWNL
                     GGRLHFNSGRPYTTPSAGQSVSEALTYNRNNSRLPSFFQLDLRAERTWRFRTWNLQLI
                     VDVLNSTYVREVFACVLSDTSGMFSGSGANGNGVMGMVAAGCNQQGLRYILPSIGLRG
                     VF"
     gene            complement(5104..5496)
                     /locus_tag="VGB85_12285"
     CDS             complement(5104..5496)
                     /locus_tag="VGB85_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134859.1"
                     /translation="MLGPLRTLISVAFLVVFLWAAFTVKLGRLTFAEHVDRIGQTPEA
                     TELLDGARSRVAPTLDEVKQRVLGEYVEAPTHPAMPPAPRSTPAPKPREPAAALVSTS
                     RAASDPEPRRSRASTSGAAKLPGRRKQP"
     gene            complement(5637..6464)
                     /gene="proC"
                     /locus_tag="VGB85_12290"
     CDS             complement(5637..6464)
                     /gene="proC"
                     /locus_tag="VGB85_12290"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEY0134860.1"
                     /translation="MSDTRLAIIGCGIMGEAIVGGLLRAGLFSPGEVLATTRRQDAAD
                     DVQARHGVAVTLDNLAACRSAHTVLLTLKPQRIAKVVHEPGIREALAGKLVISIAAGV
                     TLAQLAEWLPESSVIRAMPNTPCLIGEGMTVLARGKGVADAEVAIAARLFETVGRCIE
                     VEDKLMDAVTSLGGSGPAFVYIMIEAMADGGVMMGLPRPVALEIAAQVFQGAARMVLQ
                     TSRHPAALKDQVTTPAGCTIAGVLTMEDGRIRSVLARTIQEATRVAGELGKPTVADR"
     gene            complement(6486..>6869)
                     /locus_tag="VGB85_12295"
     CDS             complement(6486..>6869)
                     /locus_tag="VGB85_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134861.1"
                     /translation="DACEDSQYLVLCLACSRCEQCFACVGLQGESYCILNQRYSRKDY
                     FIIVQGLRKRLEEQRASLLSELAAASRGLWTGTGAPTGKFAVTGGGVRAVLDGNVPPP
                     EELAPVDPADEDPRQDGVVPARFDG"
BASE COUNT         1039 a         2302 c         2468 g         1060 t
ORIGIN      
        1 tggacgtcct catcgtcacc gacgagtccg gcatcctcgt gtcgagcagc accaccgagc
       61 tcgacctcgc catgctcgcc gcggtcaccc cgctggtcgc tcgtggcagc gccaaggcca
      121 acatcaagcg cgacggtgcc atgcgcgagt acagcgtccg cagcatcaag gtgctcggcg
      181 agacgctcta cgtcggcgcc ctcggcggga agttcggccc gcgtgagcgc gagctggcga
      241 ccaccgcctc cgcggcgcgc cgcatcctcc tcagctagcc tgtccttgcg gacagctacc
      301 ccgcggtcga gcgcgccagg tcggcgagcg cggcctgcag gcccacgtcc ggctggtcgg
      361 gctgcgtcgg cgggaccggt ggcaaggcga catggaggtc cgggtcgacg ccgttgccat
      421 cgatcagccg gtccttcggc gagaagtagc gcgcgatcgt cagcgtcagc agcgagccgt
      481 cgtgcagccc gatgacatcc tgcaccgtcc ccttgccgaa gctgcgctcg ccgacgacgc
      541 gggcgcgccc gtggtcttgc aaggcgacgg cgagcagctc cgaggccgac gccgatcgct
      601 ggtcctgcaa caccaccagg ggcaacgcgc tgtcgctgcc gtccgggtgg gcccgttctt
      661 ccctcaagat ctgcccgccg cggccgcggg tccgcaccag cacaccgtcg gcgacgaaca
      721 tgtcggcgat catcaccgcc tccgggacat caccacccgg gttaccgcga aggtcgagca
      781 ccaggccggc gagcgtcgag ccggccgcgc ggcgcatgtc cttgaggctg cgcttgacca
      841 gctccgcggt gccgccacga aacgcccgca cgcgcaggtg ggcgaacttg cggccgtcgt
      901 gatcgatcac cctgccctcg acgaggttct tcggctgccc ggtgagcacc aggcgaacgg
      961 cgatcggcgc ggggtcacta cgccgctgca ccgacatctc gagcgtgtcg ccgtcggcgc
     1021 ccatcaacag cgccgcagcc tcggcctgcg acagcaaaaa accaacgtcc tgcccgcgaa
     1081 tctttaagat ttgatcgccg gggtgtaggc ccgcggcctc ggcgggtgag ccgggaagca
     1141 ccgcgctgat ctcgagcgac gccggcttgc gagcgccggg ctcggcgcga tgcagcagcg
     1201 tctccatgcc cgtgctgccg ccacggctgc gctgacggat ctcggcccgc tcgtcggcgg
     1261 tcaaaaagtg gctgtaggtg tcgaggccgc tgacgatccg cttgagggtc tcggtcagca
     1321 ccggcgacga ctcgaccggg tcaacgtatc gctcgagtac ggtgcgcagc gccgcgtcga
     1381 agcctgcacg actgagggtg tggctgccgg cctcggcctg gcgctcgggc catccttgtt
     1441 gcaccgccca catgaagagg gctcccagcg cgaacgcgag tccgctgcca cgttggatca
     1501 ttcgggccat ggtggcccag gaacgcgtta cgatccagcg gcggtcttcg ctcgcttcac
     1561 gcggcctggg gagtggcgat gacgggggtc tcgcgcggag cggggaccgg cagcggcgcg
     1621 gagacgagac gggcgatgcg aagacgcgcc tgcttcatct ccatcagggt cgtccagaac
     1681 ggacggacgg tggcggcggt gaccatggag tggatgaaag cgtcgggacg gttgcggagc
     1741 atgaccgtac tcgtgtgcat ggtctgttcc aggtgtcgcg acgggattgc gagaatttat
     1801 gaaaatcgag gcgcggcgcc agcttgcttc caggatcagg gttctcccgg cgccgccagc
     1861 gcgaggcttg tcagcttggg ccggccagcc cgtgggacac gacgacatcg actgtcacat
     1921 gtggatgacg cgatgccatc gatagcgtcg tgttttggat acgcgggttt tcgtccggcg
     1981 ggcgggccgg gtgtcccgcg ggtgggacat ccctccccat tttaaaaaat gaaagagagc
     2041 agcgagcctc gcggctggct gctctctttt taaaaaggca cgcggctggg gagcggtcag
     2101 ccgggttcca tcccgcgggc ggacttcacg agctcgcgga tctgcttcgg ggacagctgg
     2161 gcagcgacgg cgttgagctc gtcgagcagc tcggccttgg cgccgccctt ttgcttggcg
     2221 cgcgggagca gctcggtcgg gtcgcaaccg aaggcggtcg ccagcagcag gagggactcg
     2281 atgcgtgggg ccttgtaacc gagctcgagc ttgttgacgg tcgtgaccgt caagccggtc
     2341 tgcttggcga gctcctcgat cgtcatgtgg ggtcgccagc cggtctcacc ctgattcttg
     2401 cggcgggtct tcgggggagg agtcgcggtc cgccacagtc tgatccttcg tccgatttcc
     2461 tgcgctactt cgttcatgat gagctcccgt tgagatgcct ggcgcggcat gatgacacat
     2521 ctcatttcgt cacctgaacg gtccgcaaat gcggatgctc acgtgaacag tcctttcgca
     2581 ccggtacctt gggtcagctt cacctgtccc ttggaacagg cggcttaatt ttcaaaacac
     2641 cccgcgcagg ccgatcgacg gcaagatgta gcgcagccct tgctggttgc aacccgctgc
     2701 gaccatgccc atcacgccgt tcccgttcgc gcccgacccg gagaacatgc cacttgtgtc
     2761 cgagagcacg caggcgaaca cctcgcggac gtaggtcgag ttgagcacgt cgacgatcaa
     2821 ctgcaagttc caggtccgga accgccaggt ccgttcggcg cgcaggtcga gctgaaagaa
     2881 cgagggtaag cggctgttgt tgcggttgta ggtcagcgcc tcggagacgc tctggccggc
     2941 cgagggcgtc gtgtacgggc gaccgctgtt gaagtgcagg cgcccgccga ggttccactt
     3001 ctcgccgatg cgatagccga gcacgatgtt gaggatgtgc cgctggtccc agttcgacgc
     3061 cgactcggtg ccgttctcga aggtgcgagt cgagcgagac agcgtgtacg cggcccagcc
     3121 gtacacgcgc tggcgcgggg cgaggcgcag catggtctcg aggccgtaag cgaggccctt
     3181 cgcgcggatg acgaggtcgt cgaactcgga gatgttgccc tgcgcggcgg cggcgaggtc
     3241 gtagtcctgc aggttgtcga ggcgcccgag gtaggcgtcc tgggtgagga tcgcacggtc
     3301 ggcgatcgag aactcgtagc cgaggctgcc ctggatgttg cgctgcaggc cgcgctcgaa
     3361 gccgaagctc tcgacgccgg ggatcgggac ggggaagctc ggcggctggt ggtagacgcc
     3421 gaagttctgg cgcagggtcc agcggtcgtt gatcttctcg cggaacagaa ttcgcgggtc
     3481 gacgccgatc gcgtgcgtga cgctcgtcgc ggtcggcagc accggcgagg gtccgatctg
     3541 cgcgtagatg tccgtgcgca cgccgggccg gacctcgatc gggccctttt tccacagcag
     3601 ctcggcgtag aggccggtcg ccgagacgaa gcggtcgccg agcagcaccg acaggtaatc
     3661 ggtgacgctc gggctgctga cgttggtgtc ggagcgaaag atctggaact cttgatcgac
     3721 gccgaagccg aactcgccgt tgctgcgctc gcccagcggc aggcggacgt cgatgcgcgg
     3781 ggagatctgc cactccgccg tgcggatcgg tgaggcggcg acctggctcg gcgtgacacc
     3841 gctgcggtcg agcccgagcg cgacgctgta caccgccgtg ccgccgcgcg gcagcgactg
     3901 acgcacgcgc tgctggatcc ggtagaactc gaagcgcacc agcgggtcgg ggctgagctt
     3961 gtcaccgagc gagtcgaagc tgccgtacgc gaaggcggtg tactgcgcgc gctggccgag
     4021 cttgtgatcg aagcgggcct gatagtccca gaagttgatc ttcgcggtcg actgcacgag
     4081 cgtcaacacg cccgcggtgt agctgtagcg gcccgccagc gtcagcgagc cgcgcgcctc
     4141 accgcgccgg cagtcgagct tggctcgcgc cttcgcgtcc atctttccgt gcttcttctg
     4201 ccggcgacac gccgcgtcgc gcctgcggtt gagcgggacc tcgacgatgc cgccggcgtc
     4261 ggtgatgcgg atgtcgaact cgccgaacac gcggtcacgc cgggccgcct tggtgcgccc
     4321 ctcgatcatg ccgctgacgt aacgaccgag ccgcaccggg gcgccgccgg gataaaagtc
     4381 gacctggtcg atgaagtacg ggtggatgac cgacgggccg atcgccacgt ggaacaggat
     4441 cggcacgcgc acgccgtcga ggaagtagcc ggtgttgccc ggcgccgcgc cgcgcacgac
     4501 cacgtacggg agaaagccag ccagctgcga ggcgcccggc aggctcttga cgacgcggaa
     4561 cgggtcgcca aacgtgccgg gcagcgcgtg gatctcgtcg tcgcgcaggt tcgtgctcga
     4621 gatcgcgacc tcgcgctgct ggcgaaccac cgtgcggtac gggttgccgt cgaggtccgg
     4681 cgcgaggcga tactcgtaga cgaggtcctg accgtcgacg agcttgacct tgatcggcag
     4741 ctcacgaaac ccggcggccc ggatcgtcag ggcgtgctcg ccaggcggca accacagctc
     4801 gaagcgaccc tcggcgtcgg tcgccgtggc gatgtcggcg gtggtcccga ggatccgcgc
     4861 gccgaacacg acgtcgcggc tgccgtacac cagcacctga ccggacagcc gcgcgcgacc
     4921 cgtcaccggc gccgcgaagg ccggcgcgga cctggcctcc gggacaggtc cgctcgtcgg
     4981 cgccgcggcc atcacggcga ggccggcgag gacgagggcg gcgaggccgg acaccggcgc
     5041 aggatagcgg acctggccgc gtccgcgggc gaccggttgc tccgaaccag cgcaggcggg
     5101 cgctcagggc tgcttgcggc gtccgggcag cttcgcggcc ccggaggtcg aggctcgcga
     5161 gcggcgcggc tccgggtcgc tcgcggcccg ggaggtcgag acgagggccg cggccggctc
     5221 ccgcggcttc ggcgctgggg tgctgcgcgg cgcaggtggc atggcggggt gggtgggcgc
     5281 ctcgacgtac tcgccgagca cccgctgctt gacctcgtcg agggtcggag cgacgcggga
     5341 gcgggcgccg tcgaggagct cggtggcctc gggggtctgg ccgatgcgat cgacgtgctc
     5401 ggcgaaggtc aggcggccga gcttcaccgt gaacgcagcc cagaggaaca cgaccagaaa
     5461 ggccacgctg atcagggtgc gcaggggacc gagcacggcc gccgcagcct gccgcagccg
     5521 gggcagcccg gcaaattacg cgcgcgatcg gccgtcctgg tcgcccgcag tctcgcctcg
     5581 acggtcggcg acggtcggct tgccgagctc gccagcggtg gcccgggcgt ctcgactcaa
     5641 cgatcggcga cggtcggctt gccgagctcg ccagcgacgc gggtggcctc ctggatcgtc
     5701 cgcgcgagca cggaacggat ccgcccgtcc tccatcgtca gcacgccggc gatcgtgcag
     5761 cccgcggggg tggtcacctg gtccttgagc gctgccgggt gcctgctggt ctgcaggacc
     5821 atgcgcgcgg ccccttggaa cacctgcgct gcgatctcga gggcgaccgg gcgcggcaga
     5881 cccatcatca cgccgccgtc ggccatcgcc tcgatcatga tgtacacgaa cgcggggccg
     5941 gagccgccga ggctggtgac ggcgtccatc agcttgtcct cgacctcgat gcagcggccg
     6001 acggtctcga acagccgcgc cgcgatcgcg acctcggcgt cggcgacccc cttgccgcgc
     6061 gcgagcacgg tcatgccctc gccgatcagg cacggcgtgt tcggcatggc gcggatcacc
     6121 gaggactccg gcagccactc ggcgagctgc gcgagggtga cgccggcggc gatgctgatc
     6181 acgagcttgc cggcgagcgc ctcgcggatg ccgggctcgt gcacgacctt ggcgatccgc
     6241 tggggcttga gcgtcagcag cacggtgtgc gccgaccggc acgcggcgag gttgtcgagc
     6301 gtgacggcga cgccgtgacg cgcctgcaca tcgtcggcgg cgtcctggcg gcgcgtggtc
     6361 gcgagcacct cgccggggga gaacagcccg gcgcgcagca gcccgccgac gatcgcctcg
     6421 cccatgatcc cgcagccgat gatggcgagt cgcgtgtctg acatgcgccg gatgctaatg
     6481 ctttctcagc cgtcgaagcg cgccgggacg acgccgtcct gcctgggatc ctcgtcggct
     6541 gggtcgaccg gcgcgagctc ctcgggcggc gggacgttgc cgtcgagcac cgcccgcaca
     6601 ccaccaccgg tgaccgcgaa cttgcccgtg ggcgccccgg tgccggtcca caacccccgt
     6661 gaggcggccg cgagctcgct cagcaggctg gcgcgctgct cctcgagccg cttgcgcagg
     6721 ccctggacga tgatgaagta gtccttgcgc gagtagcgct gattgaggat gcagtacgac
     6781 tcgccctgga ggccgacgca ggcgaagcac tgctcgcagc gggagcaggc caggcacagc
     6841 acgagatatt gggaatcctc acaagcgtc
//
LOCUS       DASWNJ010000734         1589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_190284, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000734 DASWNJ010000000
VERSION     DASWNJ010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190284"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VGB85_12300"
     CDS             complement(<1..209)
                     /locus_tag="VGB85_12300"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEY0134862.1"
                     /translation="MSVKVQGPGTGHDLRVGIIGAGCAGLAAAEELRALGYRRVTILE
                     ARARAGGQCVSETTEDADGRPVIYD"
     gene            complement(248..1468)
                     /locus_tag="VGB85_12305"
     CDS             complement(248..1468)
                     /locus_tag="VGB85_12305"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEY0134863.1"
                     /translation="MTIDASLSVVRSGLDRLARGDGPDLRGRPVALLCHPASVTADLA
                     HATDVLAGPVGATLVSLLGPEHGLDAAAQDMETVTQGTGTSGHVPTYSLYGDDFASLS
                     PTPAMLHGAQWLVIDLQDVGARYYTYVWTAVLAAEVALAAGVRVLLLDRPNPLGGLET
                     AIEGGAIDPGEESFVGLHDVAVRHGMTLGELARMAIRERGRVAHDGLAVLECAGWRRS
                     MLAPATGLPWVLPSPNMPTLDTALVYPGQCLLEGTNLSEGRGTTRPFEIFGAPWLDGH
                     ALADALKAEPEAIPGLAVRPLGFKPMFQKFAGQRCGGVQLHVREPAAVRSLQTTWAIL
                     KAAWRLGQGAMRWRTERYEFVDDRPALDLLAGGPWLRAAVEAQASLADMLATQEPGRR
                     AFAVRRRPFLLYPD"
BASE COUNT          220 a          599 c          556 g          214 t
ORIGIN      
        1 ctgtcataga tcacgggtcg cccgtcggcg tcctcggtgg tctccgagac gcactgacca
       61 cccgcgcgcg cacgtgcctc caggatcgtc acccggcgat agccgagcgc ccgcagctcc
      121 tccgcggcgg ccaatccagc gcaaccggcc ccgatgatcc cgacgcgcag atcatggccc
      181 gtcccgggcc cctgcacttt tacgctcacg gccggggatg ctaccggagc gcccatgatg
      241 cgcggtttca atcgggatag agcaagaacg ggcgtctacg cacggcgaac gctcgccggc
      301 cgggctcctg cgtcgcgagc atgtctgcga gtgatgcctg ggcctcgacc gcggcgcgca
      361 gccacggtcc gccagcgagc aggtcgagcg ccggccggtc gtcgacgaat tcgtagcgtt
      421 cggtgcgcca gcgcatcgcc ccctgcccca gccgccacgc cgccttgagg atcgcccagg
      481 tcgtttgcag gctgcgcacg gcggccggct cgcggacatg caactgcacg ccgccgcagc
      541 gctggccggc gaacttctgg aacatcggct tgaacccgag gggccgcacc gcgagccccg
      601 ggatcgcctc gggctcggct ttgagggcgt ctgcgagggc atgaccgtcg agccacggcg
      661 ccccgaagat ctcgaacggc cgggtcgtcc cgcgaccctc gctgaggttg gtgccctcga
      721 gcaggcactg ccccgggtac accagcgcgg tgtcgagggt cggcatgttc ggcgagggca
      781 gcacccacgg cagcccggtc gccggcgcca gcatgctccg gcgccagccg gcgcactcga
      841 gcaccgcgag cccgtcgtgc gcgacccggc cgcgctcgcg gatcgccatg cgcgcgagct
      901 cgccgagcgt catgccgtgg cgcaccgcga cgtcgtgcag gccgacgaag ctctcctcgc
      961 cgggatcgat cgcgccgccc tcgatcgccg tctcgaggcc gccgagcggg ttcgggcggt
     1021 cgagcagcag cactcgcacg ccggccgcga gcgcgacctc ggcggcgagc actgccgtcc
     1081 acacgtaggt gtagtagcgg gcgccgacgt cctggaggtc gatcaccagc cactgcgccc
     1141 cgtgcagcat cgccggggtc gggctcaggc tggcgaagtc gtccccataa aggctgtacg
     1201 tcgggacatg tccgctcgta cctgtcccct gggtcacggt ctccatgtcc tgcgccgccg
     1261 cgtcgaggcc gtgctcgggg ccgagcaggc tgaccagcgt cgcgccgacg ggcccggcga
     1321 gcacgtcggt cgcgtgcgcg agatcggccg tgaccgaggc ggggtgacac agcagcgcga
     1381 ccggtcgtcc gcgcaagtcc gggccgtcgc cgcgggccag gcggtcgagg ccggagcgca
     1441 caacagagag cgaggcgtcg atcgtcaccc ggcgaggctt acgcgacctc cccgatcccg
     1501 cgaagttttt ggccggtccg cgaacgcgtg cgaccttcgc tggcatgcgc gacccgctgg
     1561 caccgatccc ctcgcctgcc cgcgatccg
//
LOCUS       DASWNJ010000735         2576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_578831, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000735 DASWNJ010000000
VERSION     DASWNJ010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578831"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VGB85_12310"
     CDS             <1..1033
                     /locus_tag="VGB85_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134864.1"
                     /translation="AGARPRCNPWPCSEPPIGNTSWVGMNPHDTVTQESWNEPTLLSN
                     GHYMRFIGATCSGSSMFKFNATVHGELVFYKSSTGGNNPSNLIRDEAVEGCKFYAQYA
                     LSASFVNPQTVPILIGDAVKFKSADGSTDNFKYMMLVANLNTDLPVFNEIYHPTCYDN
                     ADAQGNYYLQIRPGLALDRNNWRFIGDATKQSWVCTAGAFGHFGLLGVDPEDISDPLL
                     GTTEGRAWGLYHHGASHTFVGNPIRIHHPTIPGYETAPNSCNPATKDWYREALWSGGN
                     LICRGSDANSWSDEINRNAWSKNLDWDHDSSVASVSVCSGSPAHDFETYAQCYMHGTT
                     RVCPINNEC"
     gene            complement(1053..1589)
                     /locus_tag="VGB85_12315"
     CDS             complement(1053..1589)
                     /locus_tag="VGB85_12315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEY0134865.1"
                     /translation="MSTPLHVLAISGSLRAASSNTALLRAAARLAPQHVTVTLYGGLA
                     ELPPFNPDHDEAPGPAVTALRAQLKAADGVLISTPEYAHGVPGSLKNLLDWVVGSGEL
                     VDKPVALLHASRSPWALASIRETLSVMSARLVEGSLTVELGSNRIDEAAVLAEPTLAA
                     PIAAALAAFAAAIAAPRA"
     gene            1651..>2576
                     /locus_tag="VGB85_12320"
     CDS             1651..>2576
                     /locus_tag="VGB85_12320"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY0134866.1"
                     /translation="MSTIRPDSSSSRACGAVALVLAALPAPALASPYARTVQEAPART
                     LTAAEPIAEPILSPEPDEDAIRTTGLPGADAATRGDASAAVASGATANAEQPFRPWEV
                     GGFIDTNYSFNSNLPDSHVNRMRSAVPRTGEFSLNLVVAYIRREAIRGQFSPTFELAL
                     QAGPAADALMYYEPKPGGDASRFAGAEVFKHIGRANVGFKTKRGTELSVGMHLSPIGI
                     GIHWTPFNWNYTVSWELDAVPYYLAGLKLVHTIDDRHGLQLWVVNGWQTLADANKVPS
                     LMLGYTFTPSPRFNLATFAYAGPDQTDVRMAA"
BASE COUNT          433 a          946 c          844 g          353 t
ORIGIN      
        1 cgccggcgcc cggccccgct gcaacccgtg gccctgctcg gaaccgccga tcggcaacac
       61 cagctgggtc ggtatgaacc cgcacgacac ggtcacgcag gaatcctgga acgagccgac
      121 tctgctgtcc aacggtcact atatgcgctt catcggcgcg acctgctcgg gcagcagcat
      181 gttcaagttc aacgccaccg tgcacggcga gctggtgttc tacaagtcga gcaccggcgg
      241 caacaacccc tcgaatctga tccgcgacga ggcggtcgag ggctgcaagt tctacgcaca
      301 gtacgcgttg agcgcgtcct ttgttaaccc gcagaccgtc ccgatcctca tcggcgacgc
      361 ggtcaagttc aagagcgccg acggatcgac cgacaacttc aagtacatga tgctggtggc
      421 caacctcaac accgacctgc cggtcttcaa cgagatctat cacccgacct gctacgacaa
      481 cgcggacgcc cagggcaact attacctgca gatccgtccc ggcctcgcgc tcgaccgcaa
      541 caactggcgg ttcatcggcg acgcgaccaa gcagtcgtgg gtgtgcaccg cgggcgcctt
      601 cggccacttc gggctgctcg gcgtcgatcc cgaggacatc agcgacccgc tgctcggcac
      661 gaccgagggc cgcgcctggg gcctctatca tcacggcgcc tcgcacacct tcgtgggcaa
      721 cccgatccgc atccatcacc cgacgatccc gggctacgaa accgccccca acagctgcaa
      781 cccggcgacc aaggactggt accgcgaggc cctgtggtcc ggcggcaacc tgatctgccg
      841 cggctcggac gccaactcgt ggtcggacga gatcaaccgc aacgcgtggt ccaagaacct
      901 cgactgggac cacgactcct ccgtcgccag cgtcagcgtg tgcagcggct cgcccgccca
      961 cgacttcgag acctacgcgc agtgctacat gcacggcacc accagggtct gcccgatcaa
     1021 caacgagtgc tgagccgcgc cgcggacgac cgtcaagcgc gtggagccgc gatcgcggcc
     1081 gcgaacgcgg cgagggccgc cgcgatcggt gccgcgaggg tcggctcggc gagcacggcc
     1141 gcctcgtcga tgcggttgct gccgagctcg acggtcagcg agccctcgac caggcgcgcg
     1201 ctcatcaccg acagcgtctc gcggatcgac gccagcgccc acggcgagcg cgaggcgtgc
     1261 agcagcgcga ccggcttgtc gacgagctca ccgctgccga ccacccagtc gagcaggttc
     1321 ttgagcgagc ccggcacccc gtgcgcgtac tccggggtcg agatcaggac gccgtcggcc
     1381 gccttcagct gcgcgcgcag ggccgtgacc gcggggccag gggcctcgtc gtggtccggg
     1441 ttgaacggcg gcaactccgc gagcccgccg tagagggtca ccgtgacgtg ctgcggggcc
     1501 aggcgggcgg ccgcccgcag cagggcggtg ttcgacgagg cggcgcgcag gctgcccgag
     1561 atggcgagga cgtggagggg cgtggacatg gacggcgcct gtgcgagcac gaccgggaga
     1621 ggtatacagg tcgccgagcg acctggctgc gtgagcacga tccgccccga ctcctcctca
     1681 tcacgcgcct gcggggccgt cgcgctcgtg ctcgccgcgc tgccggcccc ggcgctggcg
     1741 agcccgtacg cccgcacggt gcaggaggca ccggcgcgga cgctcacggc cgcggagccg
     1801 atcgcggagc cgatcctctc gccggagccc gacgaggacg cgatccgcac gacgggcctg
     1861 ccgggcgccg acgcggcgac gcggggggac gccagcgcgg ccgtcgcgtc cggggcgacc
     1921 gcgaacgcgg agcagccgtt tcgaccgtgg gaggtcggcg gcttcatcga caccaactac
     1981 agcttcaaca gcaacctgcc cgacagccac gtcaaccgga tgcgctcggc ggtgccgcgc
     2041 acgggcgagt tctcgctcaa cctcgtggtc gcgtacatcc gccgcgaggc gatccgcggg
     2101 cagttctcgc cgaccttcga gctcgcgttg caggccggcc cggccgccga cgcgctgatg
     2161 tattacgaac cgaagcccgg cggcgacgcc tcgcgcttcg ccggcgccga ggtgttcaag
     2221 cacatcggcc gggccaacgt cggcttcaag accaaacgtg gcaccgagct gagcgtcggc
     2281 atgcacctgt cgccgatcgg catcgggatc cactggacgc ccttcaactg gaattacacc
     2341 gtcagctggg agctcgacgc ggtcccgtat tacctcgcgg gcctcaagct cgtgcacacg
     2401 atcgacgacc gccatggtct gcagctgtgg gtcgtcaacg gctggcagac gctcgccgac
     2461 gccaacaagg tcccgagcct catgctcggc tatacattta caccgagccc gcgcttcaac
     2521 ctcgcgacct tcgcctacgc cggccccgac cagaccgacg tgcgcatggc cgcctg
//
LOCUS       DASWNJ010000736         3591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_296867, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000736 DASWNJ010000000
VERSION     DASWNJ010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296867"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VGB85_12325"
     CDS             complement(<1..406)
                     /locus_tag="VGB85_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134867.1"
                     /translation="MSVWHRDQVLDAARADNRALQAEDAWYGERLAEHHADLAALDAR
                     VAEAWEHLGAVLVPELAPGLLDALARQIGLPAIRAAAVESGTMQEVARQQALLASVEA
                     EPEYQNREGIKLECEMVLAECEDVLAPLRDVYA"
     gene            complement(403..744)
                     /locus_tag="VGB85_12330"
     CDS             complement(403..744)
                     /locus_tag="VGB85_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134868.1"
                     /translation="MLGEAESCELLHALFRARGYQTRADVAFAEQGVEFDADGWDPDA
                     RVGFEFLCSAAADHDDLSPDELARLAARMERGELFFFVIDESEVPDAQTLQWAAGRFL
                     DEVERRRGGAT"
     gene            complement(867..1907)
                     /locus_tag="VGB85_12335"
     CDS             complement(867..1907)
                     /locus_tag="VGB85_12335"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0134869.1"
                     /translation="MGTSGIVGSATAGLLVERGWTVHGLARRPTAQAGVVPVAADLQD
                     PASLASVLRDIRPEVVFIATWSRQATEAENIRVNAAMVRNLLAVLGPAGSLRHVALVT
                     GLKHYLGPFEAYGQGVLPQTPFREEQGRLDVANFYYAQEDELFAAADRDGFGWSVHRP
                     HTIIGQAVGNAMNMGTTLAVHATLCRETGRPFRFPGSAAQWNGLTDMTDARLLARHLL
                     WAVETPAARDEAFNVVNGDVFRWSWMWGRIAGWFGLESAPFDGTIQPLEEQMRGDGPT
                     WARIAERERLAEPDLGRLASAWHTDADLGRPIEVVTDMSKSRRLGFTAYQPTDDAFFE
                     LFRGLRAQRLIP"
     gene            complement(2345..3157)
                     /locus_tag="VGB85_12340"
     CDS             complement(2345..3157)
                     /locus_tag="VGB85_12340"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY0134870.1"
                     /translation="MLVNSKVIAARFAGEQEVDRDVAALAYHGLLLGSFALLCQGLLP
                     PAVFMIVGLCAYIRNFNAIHEGSHARKAAWNPLRPLRQAAMIVHGPLQLGRRELSKNH
                     RLHHAFPGDPQRDPDAAVNSGPWWRAFAAAFFQPELTTVDFVRRAGEIDPALRGALIY
                     NAAVSAGLLALAGVDIVWWIAVTRLGSTACWFIFDWALHHPAVYDRGELPLPRPVQLL
                     WRVMFSRDNLNAVQHHALHHRYSFVADRELPALARLLADERRTAADAAVPVA"
BASE COUNT          534 a         1338 c         1227 g          492 t
ORIGIN      
        1 ccgcgtacac gtcgcgtagc ggcgcgagca cgtcctcgca ctcggcgagc accatctcgc
       61 actcgagctt gatcccctcg cggttctggt actcgggctc ggcctcgacc gaggcgagca
      121 gggcctgctg gcgcgcgacc tcctgcatgg tgcccgactc gaccgcggcg gcgcggatcg
      181 ccggcaggcc gatctgccgc gccagcgcgt cgagcagccc cggcgccagc tcggggacca
      241 gcaccgcgcc gaggtgctcc cacgcctcgg cgacgcgggc gtcgagcgcc gcgaggtcgg
      301 cgtggtgctc cgcgaggcgc tcgccgtacc acgcgtcctc cgcctgcagc gcccggttgt
      361 cggcccgcgc cgcgtcgagc acctggtccc tgtgccagac gctcacgtcg cccccccgcg
      421 gcgccgctcg acctcgtcga ggaagcgccc ggccgcccac tgcagcgtct gcgcgtcggg
      481 cacctcggac tcgtcgatga cgaagaagaa cagctcgccc cgctccatcc gcgcggccag
      541 gcgcgccagc tcgtcggggc tcaggtcgtc gtggtcggcc gccgccgagc acaggaactc
      601 gaagccgacg cgggcgtccg ggtcccagcc gtcggcgtcg aattcgaccc cctgctcggc
      661 gaacgcgaca tccgcccggg tctggtaacc gcgggcccga aacagcgcat gcaacagctc
      721 gcagctctcc gcctcgccaa gcatcacggg ccaggatggc aggcacgcgc accgctgtaa
      781 agccgacgaa aagccgacga aaagccgagg ccaacgcgcg cgacggcgtg cgggcacgat
      841 aacgtcacac gcccggtgag ccgtcgctac gggatcagcc gctgcgctcg caggccccgg
      901 aacagctcga agaacgcgtc gtcggtcggc tggtacgccg tgaagccgag ccgacggctc
      961 ttgctcatgt cggtgacgac ctcgatcggg cggcccaggt cggcgtcggt gtgccaggcc
     1021 gaggcgagac gcccgaggtc gggctcggcg agccgctcgc gctcggcgat gcgtgcccac
     1081 gtcgggccgt cgccccgcat ctgctcctcg aggggctgga tcgtcccgtc gaagggcgcg
     1141 ctctcgaggc cgaaccaccc cgcgatccgc ccccacatcc agctccagcg aaacacgtcg
     1201 ccgttcacga cgttgaacgc ctcgtcgcgg gcggcgggcg tctccaccgc ccacagcagg
     1261 tgcctggcga gcagccgcgc gtcggtcatg tcggtcaagc cgttccactg cgcagcagag
     1321 ccggggaagc ggaacggccg ccccgtctcg cggcacagcg tcgcgtggac cgcgagggtc
     1381 gtgcccatgt tcatcgcgtt gccgaccgcc tgtccgatga tcgtgtgcgg tcgatggacg
     1441 ctccagccga agccgtcgcg gtcggccgcc gcgaacagct cgtcctcctg cgcgtagtag
     1501 aagttcgcga cgtcgaggcg cccctgctcc tcgcgaaacg gcgtctgcgg caatacgccc
     1561 tgaccatagg cctcgaacgg ccccaggtag tgcttgagcc ccgtgacgag cgcgacgtgg
     1621 cgcagactcc ccgccggccc gagcaccgcc aggaggtttc ttaccatggc cgcgttgacg
     1681 cggatgttct ccgcctccgt cgcctgccgg gaccacgtgg cgatgaagac cacctcgggt
     1741 cgaatgtctc gaagcacgct cgcgagggac gcagggtcct ggagatcggc ggccaccggg
     1801 acgaccccgg cctgcgccgt cggacgccga gccaggccat gcaccgtcca cccgcgctcc
     1861 acgagcagcc ccgccgtcgc gctgccgacg atcccgctcg ttcccacgac caaggccttg
     1921 cccgacattt tccaaggaat acgaggcggg cctgcgtgtc gtcaaggggc gccccacgcg
     1981 acggcgccag ccgcccgcca tccgcggcgt ggggcatcgg ccggctgata aaatgcccgg
     2041 atcgagttgc catatatttg tacccctcgc ctctcgatca tcgtcgccgc cgtccaggac
     2101 acgagacctc ctcgtatatg tgccccgacg agggcggtca ttgcacccgg cgacgccccc
     2161 aagaacgtgc gagacgggac ccccggcacc gtcagcgccg cgcacgatct ggatatgaag
     2221 gacatctctg cggcccgcac ccggtgcgta tcgagcagtc ggttcacccc gacgagcctg
     2281 gcgaagatgg cggggaggat ctcggcggcc atcccccgct tctcgtcggc ttcctgcggc
     2341 ccgctcaggc caccggcacc gcagcatccg ccgccgtgcg gcgctcgtcc gcgagcaggc
     2401 gagcgagcgc gggcagctcg cggtcggcga cgaacgagta gcggtggtgc aacgcgtggt
     2461 gctggaccgc gttgaggttg tcgcgcgaga acatcacgcg ccacagcagc tgcaccgggc
     2521 gcggcagcgg cagctcgccg cggtcgtaca cggcggggtg gtgcagcgcc cagtcgaaga
     2581 tgaaccagca cgcggtcgag ccgagccgcg tcacggcgat ccaccacacg atgtcgacgc
     2641 cggcgagcgc gagcagaccc gcgctgacgg ccgcgttgta gatcagcgcg ccgcgcaggg
     2701 ccgggtcgat ctcgccggcg cggcgcacga agtcgacggt ggtcagctcc ggctggaaga
     2761 acgcggccgc gaacgcccgc caccacggcc cgctgttgac cgcggcgtcg gggtcgcgct
     2821 gcgggtcgcc ggggaaagca tggtgcaggc ggtggttctt gctcagctcg cgacggccga
     2881 gctgcagcgg cccgtgcacg atcatcgctg cctgccgcag cgggcgcagc ggattccacg
     2941 cggccttgcg ggcgtgcgag ccctcatgga tcgcgttgaa gttgcggata taggcgcaca
     3001 gcccgacgat catgaacacg gccggcggca gcaggccctg acacagcagc gcgaagctcc
     3061 cgagcagcag cccgtggtag gcgagcgccg cgacgtcccg gtcgacctcc tgctcgcctg
     3121 cgaaccgggc cgcgatcacc ttcgagttga ccaacatcgg ggccgccatt ggaagatccg
     3181 tgccagcaaa aaaccgtgcg ccggcgcgga ccgccgtgcg gtcgtacgtc cacttccgtc
     3241 ctccctgctg gacgcctgtc ggaccgcctg gacaccgtgg tgccgccttg atgacctgtc
     3301 cttcaggaca ggtcatcgcc gccctcgcgg gtgagcgctt tccgcgtccc gacacgaccg
     3361 gcgccggcgc cacacgaagc gatcgttcct ggccaggctc gggcgcaccg cgcggcatat
     3421 cctggaccga ctccaccgcg cgcgcccgcg accgcgcgat cgcacgggcg gcgtgtaacc
     3481 tcgctcttcg cgctgtggta gcgtcgcggg atggattcgg ttcgcacgac tcttgggttc
     3541 ttgacggcca cgctcgcgct cctcggctgc ggcgacgacg ggcgggcgat g
//
LOCUS       DASWNJ010000737         4375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_154811, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000737 DASWNJ010000000
VERSION     DASWNJ010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154811"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="VGB85_12345"
     CDS             <1..998
                     /locus_tag="VGB85_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibrinogen-like YCDxxxxGGGW domain-containing
                     protein"
                     /protein_id="HEY0134871.1"
                     /translation="CDDGNADDTDDCLSTCLLAACGDGAVQANVEVCDDGDADDTDDC
                     TTLCEPPSCTDAIVSGDESDVDCGGGCMDCEAGAACSEGKDCATNVCKAGTCTLGASC
                     LEIHVSSPMAPSGPYMVDFDGDGPEPATGVECEMSTDGGGWTLVQRTVWDPAKTAALF
                     TGYADWYAKTVGTPTLGEGYRLAGRLWSGLNVKKRHMLVHRMRKAADGSSCAPLYYVG
                     SEGTYAIDNAGATLTGLLATVNMINNTLLSTKDSGPSTSCVNANGGAPWFYSGCCSTC
                     PTFQGSYWAQPHPMASYTAGAPDQYAAVQNDVCEGAPVMASQGYYGINDMAYYLR"
     gene            complement(986..2914)
                     /locus_tag="VGB85_12350"
     CDS             complement(986..2914)
                     /locus_tag="VGB85_12350"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0134872.1"
                     /translation="MRTSLRASAGLLALCVLCCDGPPKPPPPVAATLVIRDVRVFDGQ
                     RIVGPTTVSVVGDTITAIGATPADLSHAEVVDGAGQTLLPGLIDAHTHVHGPDALRQA
                     LVFGVTTELDMFMMPTVLRGLRRITGRQADLADLRSSSVLATAPGGHGTEYGLAIPTL
                     TGPDEAAAFVDARLSEGADYIKIVLDDGGGYGRRIPTLDDPTVRALITATHARGKLAV
                     VHISSRREAAAAIEAGADGLAHLFIDAPADPAIVALARERGVFILDTLPVIHSLCAPQ
                     HGAALARDPALAPALRPIDVQALARRFPVSASAGSVSASVSASAVSASAPVSASAPGC
                     ALALANARAFHGAGVPLLASTDAPNVGTVHGASLHEGLALLVAAGLTPVEALGSATAL
                     PAKIFGLQDRGVIAPGKRADLLLVRGDPTQDITATRAIVAVWRGGQRLDRQAWLAPVP
                     RLPAARAALRTAAPPPGSAAGKISDFEAGQLASEFGAGWQPATDERIGGSSTVALAVT
                     RPGAGGSAHALRMTGTVVESPAPARWGGAIFFPGATPLAPANLAGFARVSFWARSDAD
                     AQLTVMAFASQPGAQPGEQEVAVGRTWTRHSLALADFVGIEPFDLAGLFFGATAAGPF
                     TLDLDDVRLESPQADQRK"
     gene            complement(2916..>4375)
                     /locus_tag="VGB85_12355"
     CDS             complement(2916..>4375)
                     /locus_tag="VGB85_12355"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0134873.1"
                     /translation="SSGTPTCSPTACARAGWRSERKIRRFVAERLLTPDGARSQALRG
                     PKATAGLANKLVDALERERLIRSETRGGRTWVELAHDRLVPAIHLSNRTWSESQARRR
                     RAWRFVFFMLMFAGLVVGLAYLGKLAYGKWQELNNEKAGLEQKLATLGEGEEALTKQL
                     ARAQGELAGEQRRAQLARLGEELRTLARDAQLLQTVIFEMGRYRPSPRDVDVEAETLI
                     NYVASGQELARVGLQVQALGASQQQLLADIKATATTHKIDELEQLFDDLGDATEELTS
                     ELERLQKSVTTMTTDFGRYSARLSANLDGWESPPRPGASLEERVRETSRGQWRAGFRA
                     WLANDIPGARSRFQKAAERDPSNPAAHEALARLSWSEGQFAAAEKDFRAALAQGVEYG
                     PALAGMSAVYLHEQALADADRCVRRALAVQPGFAAAHFILGEVKHRLAQPDVKAGKVS
                     GDNPCKKRAAPAAPTDPAAAPADPAAAPADPAPAE"
BASE COUNT          593 a         1528 c         1696 g          558 t
ORIGIN      
        1 agtgcgacga cggcaacgcc gacgacaccg acgattgcct gagcacctgc ctgctggcgg
       61 cctgcggcga cggtgcggtc caggccaacg tcgaggtgtg cgacgacggc gacgccgacg
      121 acaccgacga ttgcacgacg ctgtgcgagc cgccgagctg caccgacgcg atcgtcagcg
      181 gcgacgagag cgacgtcgac tgcggcggtg gatgcatgga ctgcgaggcc ggcgcggcgt
      241 gcagcgaggg caaggactgc gcgacgaacg tgtgcaaggc ggggacttgc acgctcggcg
      301 cgagctgcct ggagatccac gtgtcgtcgc cgatggcccc gagcggcccg tacatggtcg
      361 acttcgacgg cgacggcccc gagcccgcga ccggcgtcga gtgcgagatg tcgaccgacg
      421 gcggcggctg gacgctggtg cagcgcaccg tgtgggaccc ggcgaagacc gccgcgctgt
      481 tcaccggata tgccgactgg tacgccaaga cggtcggcac gccgacgctc ggcgagggct
      541 atcgcctcgc gggccggctg tggagcggcc tcaacgtcaa gaagcggcac atgctggtcc
      601 accgcatgcg caaggcggcg gacggcagct cgtgcgcgcc gctgtactac gtcggcagcg
      661 agggcacgta cgcgatcgac aacgccggcg cgacgctgac cggcctgctc gcgaccgtca
      721 acatgatcaa caacacgctg ctgtcgacca aggacagcgg gccgagcacc agctgcgtca
      781 acgcgaacgg cggggcgccg tggttctact ccggctgctg ctcgacctgc ccgacctttc
      841 agggcagcta ctgggcgcag ccgcacccga tggccagcta caccgccggc gcgccggacc
      901 agtacgcggc cgtgcaaaac gacgtctgcg agggcgcgcc ggtgatggcg tcgcaggggt
      961 attacgggat caacgacatg gcctattact tgcgctgatc ggcctgtgga gattcgaggc
     1021 ggacatcgtc caggtcgagc gtgaagggcc cggcggcggt cgccccgaag aacagacccg
     1081 cgaggtcgaa gggctcgatg ccgacgaagt cggcgagcgc gaggctgtgg cgggtccatg
     1141 tccggccgac cgccacctcc tgctcgcccg gctgcgcgcc gggctggctg gcgaaggcca
     1201 tcaccgtcag ctgcgcgtcg gcgtcgctgc gcgcccagaa gctgacgcgc gcgaagccgg
     1261 cgaggttggc cggggccagc ggcgtcgcac cggggaagaa gatcgcgccg ccccagcgcg
     1321 ccggcgccgg ggactcgacg accgtgcccg tcatgcgcag cgcgtgcgcg ctgccgccgg
     1381 cgccgggcct ggtgacggcg agggcgacgg tcgagctgcc gccgatgcgc tcgtcggtcg
     1441 cgggctgcca gccggccccg aactcggacg cgagctgccc ggcctcgaag tcgctgatct
     1501 tgcccgcggc ggacccgggc ggcggcgcgg cggtgcgcag cgccgcgcgc gcggcgggca
     1561 gccgggggac gggggcgagc caggcctggc gatcgaggcg ctggcctccg cgccacaccg
     1621 cgacgatcgc ccgcgtcgcg gtgatgtcct gcgtcgggtc gccgcggacc agcagcaggt
     1681 cggcgcgctt gcccggggcg atcacgccgc ggtcctgcag gccgaagatc ttcgccggca
     1741 acgcggtcgc cgagcccagc gcctcgaccg gcgtcagccc ggccgcgacc agcaaggcga
     1801 ggccctcgtg cagcgaggcg ccgtgcacgg tgccgacgtt cggcgcgtcg gtgctggcga
     1861 gcagcggcac gccggcgccg tgaaacgccc gcgcgttggc cagcgcgagc gcgcagccgg
     1921 gcgcgctcgc agagaccggc gcgctcgcag agaccgcgct cgcagagacc gacgcgctca
     1981 cagagccggc gctcgcggac accgggaacc tgcgcgcgag cgcctgcacg tcgatcggtc
     2041 gcagcgcggg cgccagcgcc gggtcacggg ccagcgcggc gccgtgctgc ggggcgcaca
     2101 ggctgtggat cacgggcaac gtgtcgagga tgaagacgcc gcgctcgcgc gcgagcgcga
     2161 cgatcgcggg gtcggcgggc gcgtcgatga acaggtgggc gaggccgtcg gcgccggcct
     2221 cgatcgccgc ggcggcctcg cggcggctgc tgatatggac gaccgcgagc ttgccgcgcg
     2281 cgtgcgtggc ggtgatcagg gcgcgcacgg tcggatcgtc gagcgtcggg atccgtcggc
     2341 cataaccgcc gccgtcgtcg agtacgatct tgatgtaatc tgccccttcg gacaggcgcg
     2401 catcgacgaa ggccgcggcc tcgtcggggc ccgtcagcgt cgggatcgcc aggccgtact
     2461 cggtgccgtg gccgccgggc gcggtcgcca ggacgctcga gctgcggagg tcggcgagat
     2521 cggcctggcg cccggtgatc cgccggagcc cgcgcaggac cgtcggcatc atgaacatgt
     2581 cgagctcggt ggtcaccccg aacaccagcg cctggcgcag cgcgtcgggg ccgtgcacgt
     2641 gcgtgtgcgc gtcgatcagg cccggcagca gggtctggcc ggcgccgtcg acgacctccg
     2701 cgtggctcag atccgccggt gtcgcgccga tcgccgtgat ggtgtcaccg acgacgctga
     2761 cggtcgtcgg tccgacgatc cgctggccgt cgaacacgcg cacgtcgcgg atcacgaggg
     2821 tcgcggcgac cggcggcggc ggtttcgggg ggccgtcgca gcacagcacg cacagcgcca
     2881 ggagcccggc gctcgctcgc agagacgtgc gcacgctact ccgcgggggc agggtcagcc
     2941 ggggcggcgg cagggtcagc cggggcggcg gccgggtccg tcggggcggc cggggcggcg
     3001 cgcttcttac acgggttgtc gccgctgacc ttgcccgcct tcacgtcggg ctgcgcgagg
     3061 cgatgcttga cctcgccgag gatgaagtgc gccgcggcga agcccggctg cacggcgagg
     3121 gcccggcgga cgcagcggtc ggcgtcggcc agcgcctgct cgtgcaggta cacggccgac
     3181 atgccggcga gcgccggacc gtactcgacg ccctgcgcga gcgcggcgcg aaagtccttt
     3241 tcggcggccg cgaactggcc ctccgaccac gacagccgcg cgagggcctc gtgcgccgcc
     3301 gggttgctgg ggtcgcgctc ggcggccttc tggaaccggc tgcgcgcgcc ggggatgtcg
     3361 ttggcgagcc aggcacgaaa gccggcgcgc cactgcccgc gcgaggtctc gcgcacgcgc
     3421 tcctcgagcg acgcgccggg ccgcggcggc gactcccagc cgtcgaggtt ggccgacagg
     3481 cgggcgctgt agcggccgaa gtcggtcgtc atcgtggtga cggacttttg caggcgctcg
     3541 agctcggatg tcagctcctc ggtcgcgtca ccgaggtcgt cgaacagctg ctcgagctcg
     3601 tcgatcttgt gggtggtcgc ggtcgccttg atgtcggcga gcagctgttg ctggctggcg
     3661 ccgagcgcct gcacctgcaa gccgacgcgc gcgagctcct gcccggacgc gacatagtta
     3721 ataagggtct cggcctcgac gtcgacgtcg cgcgggctcg ggcggtagcg ccccatctcg
     3781 aagatcaccg tctgcagcag ctgggcgtcg cgggccagcg tgcgcagctc ctcgccgagg
     3841 cgggccagct gggcgcgccg ctgctcgccg gcgagctcac cctgggcccg cgcgagctgc
     3901 ttggtgagcg cctcctcgcc ctcgccgagc gtcgccagct tctgctcgag gccggccttc
     3961 tcattattaa gctcctgcca cttgccgtac gcgagcttgc cgaggtaggc gagcccgacg
     4021 acgaggccgg cgaacattaa cataaagaac acgaagcgcc acgcgcggcg gcggcgggcc
     4081 tggctctcgg accatgtgcg attggacagg tggatcgcgg ggacgaggcg gtcgtgggcg
     4141 agctcgaccc aggtgcggcc gccgcgggtc tcgctgcgga tcaggcgctc gcgctcgagg
     4201 gcgtcgacga gcttgttggc gaggccggcg gtggccttcg ggccgcgcag ggcctggctg
     4261 cgcgcgccgt ccggcgtcag caggcgctcg gcgacgaagc ggcggatctt ccgctcgctg
     4321 cgccagccgg cgcgcgcgca ggctgtcggg gaacaggtcg gcgtgccgct cgagg
//
LOCUS       DASWNJ010000738         3046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_49315, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000738 DASWNJ010000000
VERSION     DASWNJ010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49315"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VGB85_12360"
     CDS             <1..835
                     /locus_tag="VGB85_12360"
                     /inference="COORDINATES: protein motif:HMM:NF039902.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEY0134874.1"
                     /translation="GITDVIRGDDHLTNAFRQTQLYKALGAEPPRFAHIPLIHGPDGA
                     KLSKRHGALSVTEYREMGFLPEAVRNYLLRLGWGHGDEEVIPLERAVEWFDLDAVGRA
                     PSRFDMAKLLSLNAHWLRERPDGELAELVLPRLAGLVGAEPDAAGRERVAAGMGGLKQ
                     RARTLVELAAAAAFYVRPRPIPLDEKAAKALDDAGVRAAIGGLAGALGTVEPWEEAAL
                     EAACRAAAAERGLGFGKLAQGLRAALTGAAVSPGLFEVMRALGRAETVGRLEDAAEGA
                     Q"
     gene            957..3017
                     /gene="gltA"
                     /locus_tag="VGB85_12365"
     CDS             957..3017
                     /gene="gltA"
                     /locus_tag="VGB85_12365"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEY0134875.1"
                     /translation="MSDGAGPQVVTLSTGGDGASAELPVLKGTIGPDVIDIRKLYGQT
                     GHFTYDPGFTATAACRSKITYIDGDEGILLHRGYSIEELATKSDYLEVCHLILNGELP
                     NAAEKAEFVHNITYHTMLNEQILQLTRGFRRDAHPMAVMIAIVGALSAFYYEDLDVED
                     PHKRMIATHRLIAKVPTIAAMAYKYSVGQPIQYPRNDLDYASNFLHMMFAVPCEPYKI
                     NPVMAKALDRIFILHADHEQNASTSTVRLVGSTGASPYAAVAAGIASLWGPAHGGANE
                     AVLSMLREIGSPSRVGDYIAKVKDKDSGVRLMGFGHRVYKNYDPRAGVLKESADEVLQ
                     DLGKRDNPLLDIARELEKIALSDDYFVQRKLFPNVDFYSGLILEAMGIPTRMFTAIFA
                     LARTVGWIAQWNEMIEDPEQKIGRPRQLYTGAPARPFVTMDLGGCAAGPGRAGRDDSA
                     AHHLRGEQGLGGGLAADPGLALELPDAELAALHRYLEPELVAGSDRAAEARGLDAHEV
                     DAAAVIVGAERGQDQHGRGLGHGFDQEHARHDRVVRKVALEVGLVDRDLLDAHRAVVG
                     LHLDHAVDQQHGEAVRYPPHHALRVEILQARPYRERPRRRGRRDHLSRLPLDRPGTPD
                     STPGRRMSRGKRRAAIAMVPHRRCRRDDPTDDYCVTLSGTLTLGSFASTRLMTSSVRS
                     IWGE"
BASE COUNT          485 a         1036 c         1104 g          421 t
ORIGIN      
        1 gggcatcacc gacgtcatcc gcggcgacga ccacctcacc aacgcgttcc ggcagacgca
       61 gctctacaaa gcgctcggcg cggagccgcc gcggttcgcg cacatcccgc tgatccacgg
      121 gccggacggg gcgaagctca gcaagcggca cggggcgctg agcgtgaccg agtaccggga
      181 gatgggcttc ctgcccgagg cggtgcgcaa ctacctgctc aggctcggct ggggccacgg
      241 cgacgaggag gtcatcccgc tcgagcgcgc ggtcgagtgg ttcgacctcg acgctgtggg
      301 cagggcgccg tcccgcttcg acatggcgaa gctgctgtcg ctcaacgcgc actggctgcg
      361 cgagcggccc gacggcgagc tggcggagct ggtcctgccg cgcttggcgg ggctggtggg
      421 cgcggagccg gacgcggccg gccgggagcg cgtggccgcc ggcatgggcg gcctgaagca
      481 gcgggcgcgc acgttggtcg agctggccgc ggcggcagcg ttctacgtcc gcccgcggcc
      541 gatcccgctg gacgagaagg cggccaaggc gctcgacgac gcgggcgtac gcgcggcgat
      601 cggcgggctc gccggagcgc tagggacggt cgagccctgg gaggaggcgg cgttggaggc
      661 ggcgtgccgc gccgcggcgg cggagcgggg gctcgggttc ggcaagctgg cgcaggggct
      721 gagggcggcg ctcaccgggg cggcggtgtc gccgggactg ttcgaggtga tgcgggcgct
      781 gggccgggcc gagacggtgg gcaggctcga ggacgcggcg gaaggggcgc aataaggccg
      841 cgacgcccgc ccgttgaccg ggacttgccc cggcatagag tgtggtgcag agggtgctgc
      901 atcgcagcag cggggcgcgt gaccggggct gcccggtcga cggaaggaag agcgcgatga
      961 gcgacggtgc tgggccacag gtggtgacgc tctcgaccgg cggcgacggg gcctcggccg
     1021 aactgccggt gctcaagggg acgatcggcc cggacgtcat cgacatccgc aagctctacg
     1081 gccagaccgg gcacttcacc tacgaccccg gcttcaccgc caccgcggcc tgccgcagca
     1141 agatcaccta catcgacggc gacgagggga tcctgctgca ccgcggctac tcgatcgagg
     1201 agctggcgac caagtcggac tacctcgagg tctgccacct gatcctcaac ggcgagctgc
     1261 cgaacgcggc cgagaaggcc gagttcgtcc acaacatcac ctaccacacg atgctcaacg
     1321 agcagatcct ccagctcacc cgggggttcc gccgcgacgc gcacccgatg gcggtgatga
     1381 tcgcgatcgt gggcgcgctc tccgccttct actacgagga tctcgacgtc gaggacccgc
     1441 acaagcggat gatcgcgacg caccggctga tcgccaaggt gccgaccatc gccgcgatgg
     1501 cctacaagta ttcggtgggc cagccgatcc agtacccgcg caacgacctg gattacgcct
     1561 cgaatttcct gcacatgatg ttcgcggtgc cgtgcgagcc ctacaagatc aatccggtga
     1621 tggccaaggc cctggaccgg atcttcatcc tccacgccga ccacgagcag aacgcctcga
     1681 cctcgaccgt gcggctcgtc ggctccaccg gtgccagccc ctacgccgcg gtcgcggccg
     1741 gcatcgcctc gctgtggggc ccggcgcacg gcggggccaa cgaggccgtg ctcagcatgc
     1801 tgcgcgagat cggcagcccg tcgcgcgtcg gcgattacat cgcgaaggtc aaggacaaag
     1861 attccggcgt ccgcctgatg ggcttcgggc accgggtcta caagaactac gacccgcgcg
     1921 ccggcgtgct caaggagagc gcggacgagg tgctccagga tctcggcaag cgcgacaacc
     1981 cgctgctcga catcgcccgg gagctggaga agatcgcgct cagcgacgac tacttcgtcc
     2041 agcgcaagct gttcccgaac gtcgacttct actcgggcct gatcctcgag gcgatgggca
     2101 tcccgacgcg gatgttcacg gccatcttcg cgctggcgcg gacggtgggc tggatcgcgc
     2161 agtggaacga gatgatcgag gacccggagc agaagatcgg ccgcccgcgt cagctctaca
     2221 ccggagcccc ggcgcggccg ttcgtgacga tggacctggg gggctgtgcg gcggggccgg
     2281 gccgggccgg ccgagacgac tcagcggccc atcatcttcg cggtgaacag ggcctcggcg
     2341 gcggcctcgc cgccgaccct ggccttgccc tcgaacttcc agatgccgag cttgccgcgc
     2401 tgcaccgcta cctcgagccg gagctcgtcg ccgggtcgga ccgggcggcg gaagcgcgcg
     2461 gcctcgacgc ccatgaagta gacgcggctg ccgtcatagt cggggccgag cgaggccagg
     2521 atcagcacgg ccgcggtctg ggccatggat tcgatcagga acacgcccgg catgatcggg
     2581 tcgttcggaa agtggccctg gaagtagggc tcgttgatcg tgacctgctt gatgcccacc
     2641 gcgcggtggt aggcctccat ctcgaccacg cggtcgatca gcagcatggg gaagcggtgc
     2701 ggtatccgcc gcatcacgcc ttgcgtgtcg agatcctcca ggcgcgtccc taccgcgagc
     2761 gcccccggcg gcgtggacgc cgcgatcatc taagccgtct ccccctcgat cgacccggca
     2821 ccccggatag caccccgggc cggagaatgt cgcgcggcaa gcgccgggcc gccatcgcta
     2881 tggtgccgca ccggcggtgt cgccgagacg atcccactga cgactactgc gtgaccttgt
     2941 cgggaacctt gacgctcggc agcttcgcgt cgacgcgcct gatgacctcg tcggtcaggt
     3001 cgatctgggg cgagtagagc agcacggtcg agctcggcag gacgac
//
LOCUS       DASWNJ010000739         7302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_296922, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000739 DASWNJ010000000
VERSION     DASWNJ010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7302
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296922"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="VGB85_12370"
     CDS             complement(<1..1024)
                     /locus_tag="VGB85_12370"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0134876.1"
                     /translation="MPTTMPHEAAPETVPWREVVRGNVLAIGLVSLLTDLASEMMNPL
                     LPLFVAGLVPVGLAPVVLGLMEGVAEATASLLKLVSGRLSDRLGRRKGLVLLGYGLST
                     LVRPLMGLATGGAQVVGVKFVDRIGKGLRTSPRDALLGDAAPPSRRALAYSFHRAMDH
                     TGAVLGPLVAAGLLYTLLGYGFWRGASVVPTAEEMAALRVVFLLGLIPGLAAVAVIVL
                     GVRDKPLVATAAPGTSAPVPGDIPALPRRFYGFVGVSVVFALGNSSDLFLLLYAHERF
                     GLGMPQILGLWIALHVAKVLGSIPGGLFADRVGRRPAILAGWTLYALCYLGFGVAATL
                     SQLVLML"
     gene            1192..1551
                     /locus_tag="VGB85_12375"
     CDS             1192..1551
                     /locus_tag="VGB85_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134877.1"
                     /translation="MFPQRSLRIDLPRILVLLVLGAFLGVACGPPPGSSCRLNPLCGS
                     GDLGSTCDGNGNCRSNHCCKNDDCDGGTCTLPCGKKDPCPGGMTCHGDQCYFSCAFDG
                     DCAAGQRCKDDNFCSWD"
     gene            complement(1606..2169)
                     /locus_tag="VGB85_12380"
     CDS             complement(1606..2169)
                     /locus_tag="VGB85_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134878.1"
                     /translation="MTAPTLHPDGDRYTRRIADTSAAHQRLVASHRDFLALLQARVSS
                     HALAEDILQDAFVRCGDGTDSPSDDDSAVSWFLRNLHVAVINYQERHEVTSRGLSALA
                     ADLDGNSRAAAGLRRVVYRSLVRLVENLDPVYAVALKRTEVESVGAEEYASKAPVKNR
                     NTLVVRLFLAREALKKQVTLSCMTAIE"
     gene            2448..3014
                     /locus_tag="VGB85_12385"
     CDS             2448..3014
                     /locus_tag="VGB85_12385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY0134879.1"
                     /translation="MAAGSEETNDAALLAAAQRGERAAQTQLYNRHRERVARQVLRMI
                     GNSAAVDDLVQEVFIRAFTALPRFRGDAQLSTWLYTIATNRVRNWWDSQRRQQRRETM
                     ASGDPPDECEDPMAQIEAKEHLARFYMALGALPDRLREAFVARAIEGMSLLEASAALG
                     RPVSTVSYHTLRAEEALCTALGIVYVEK"
     gene            complement(3034..4275)
                     /locus_tag="VGB85_12390"
     CDS             complement(3034..4275)
                     /locus_tag="VGB85_12390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0134880.1"
                     /translation="MRGAGGTVGLVLAALGGIVSCAIDNPVFALAPTVVCGDGILQSG
                     EVCDDGEADETYCTAICTLPPPAPSCGDGVITAEEQCDDGNIAGGDACTERCLHAFCG
                     DSHLFADVEQCDDGNFVNDDGCLENCTVARCGDGHVQVGVETCDDGNRAAADGCDGDC
                     QFEMLESCGDGMITSGEECDDGNLDAEDGCSPACRWEIDPACGNGVVDPDEACDPTAQ
                     PYSTVVPLCLEDCSFNACLVVENTDGIDLGNNEWFSKCAFAAGDRVHVAVVSGADVIY
                     YGRGTKPRPWDPPDRLTNDGPPVQQYRLKSHQPILLDSPLGGPVGDLLYVFGRASKPP
                     IAGCEESLGDGYGLAIYGADLAPRLLLMNFLGGQSGAPRGFVGWTPAAELSFAGEDSL
                     ALCAEPGPEPLAARFVVAVTD"
     gene            complement(4418..5209)
                     /locus_tag="VGB85_12395"
     CDS             complement(4418..5209)
                     /locus_tag="VGB85_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134881.1"
                     /translation="MHVKALFPFLALSLLAGACDAAIVSQANGDAPAPPAVEAEAVVD
                     AEVAVVVAEEKAPPAGKTKVDVEIKPEALDLESITFMVKKSKVKDAAALEKKINNPKE
                     KLNNVDIDGDGKVDKIVIVEVKKDDGVIVFELHAIPSKSKDKDAAIVLAYINFTPDKT
                     TNVLIVKATYAPVVIGYDVIVYDYTVPIVVKNDVIVVSGGGGFYGWLYAVHRPAFYGV
                     VVYDVAPPPVIIVEYGYHDHGCWPPGHCKHGKWKGGKHGKHGKWH"
     gene            5497..6483
                     /locus_tag="VGB85_12400"
     CDS             5497..6483
                     /locus_tag="VGB85_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134882.1"
                     /translation="MRSWISLLAALSGGLALNCKSPEPTDTDTGSSGSGSTSTSASTS
                     APTSLDPTTTSATTGAPTTGDTSGEPVTGTGSGSTSSTSTSDTNDATTTTATTGGSSG
                     DATTMDGTTGPVGPCKPIACDGKTYACGDCEDNDSDGKVDLADSECISPCDDREDTFA
                     TGLPGDNMDPCKQDCFFDGNSGNDACDWNLACDPKNPGGDQCPYNPDKVCEPMQPAEC
                     AETCGVPNGCDCFGCCTVVVEGVSHDIFIGDKDCSVANIESCQQCTKNDDCDDECKPE
                     ECEVCFGGELPPGCEEPTCDRANPCQLDNEGNSDCPEGTFCQTGCCEPIAPG"
     gene            6551..>7302
                     /locus_tag="VGB85_12405"
     CDS             6551..>7302
                     /locus_tag="VGB85_12405"
                     /inference="COORDINATES: protein motif:HMM:NF024807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEY0134883.1"
                     /translation="MPAKSLPLRRCLLALSCLAIGGCLFTDPGPAANEGNAEASGATF
                     DAGGAEAQPTTGPQATTEPQATDGTGEPMLDCKWEQVVTSPRPAKRIDAALALGPGGA
                     QVLLYGGRVGLLGPDLADTWTFDGERWQLVNIGEEGGGGWKEQPGPRRGHAMAYDAAR
                     DQVLLFGGEFGGESGGLDVKFADLTWIHDGVQWSERKNGPFSRAFAAMADLPSSKVVV
                     LFGGRTDKGASAETWIWNGQVWAKRDFDVAPSP"
BASE COUNT         1297 a         2460 c         2377 g         1168 t
ORIGIN      
        1 cgagcatcag gacgagctgc gacaacgtcg cggccacccc gaagccgagg taacagagcg
       61 catagagcgt ccacccggcg aggatcgccg gccgccgacc cacacgatcg gcgaacagac
      121 ctccgggtat cgagcccagc accttcgcca cgtggagggc gatccacagg ccaaggatct
      181 gcggcatgcc gaggccgaac cgctcatggg cgtacagcag taaaaacagg tcgctgctgt
      241 tcccgagggc gaacacgacg gagaccccga cgaagccgta gaagcggcgc ggcagcgctg
      301 gtatgtcgcc gggcactggc gccgacgttc ccggagcggc cgtcgccacc aggggtttgt
      361 cccgcacgcc gaggacgatg accgcgaccg cggccaggcc cgggatgagt cccagcagga
      421 acacgacccg cagcgccgcc atctcctccg cagtcgggac gacgctggcg ccccgccaaa
      481 agccgtagcc gagcagcgtg tacaacaggc cggcagcgac caacgggccc agcacggcgc
      541 ccgtgtggtc catggcgcga tgaaagctgt aggcgagcgc ccgacgggac ggtggtgcgg
      601 cgtcgccgag tagggcgtcg cgtgggctgg tccgcaagcc cttgccgata cggtcgacga
      661 acttcacgcc gacgacctgc gctccgccgg ttgccagacc catcagcggg cggaccagcg
      721 tggacagtcc gtagccgagc agcacgagcc ccttgcgtcg ccccagtcgg tcactgagcc
      781 gtcccgagac cagcttgagc aggctcgccg tcgcctcggc cacgccttcc atcagcccga
      841 ggaccaccgg tgccaggccg acaggaacga ggcccgcgac gaacaacgga aggagagggt
      901 tcatcatctc gctcgccagg tcggtgagca ggctgacgag cccgatcgcc agcacgttgc
      961 cccgtaccac ctcacgccag gggacggtct cgggtgcagc ctcgtgcggc atcgtggtcg
     1021 gcatcatcac acgggaaggc cgacttatgc tcctcggacc atcgtcctgg cgttctcgcc
     1081 gccgaagtga gccgctccgg cctctcgagc ggcgatgtga cgaggccggc gggccttctc
     1141 ctggcccact cctgctttac gcgggcacca tccctgccta tcctggcgtg gatgttcccg
     1201 caacgctccc ttcgcatcga tctccccagg atactggtgt tgctcgtcct cggcgcgttt
     1261 ctcggggtcg cctgtggtcc gccgcccggt tcctcgtgcc gcctcaaccc cctgtgcggc
     1321 tcgggcgatc tgggctcgac ctgcgacggc aatggcaact gccgctcgaa ccactgctgc
     1381 aagaacgacg actgcgacgg cggcacgtgc acgttgccgt gcggcaagaa ggacccctgt
     1441 cccggcggca tgacctgcca cggcgaccag tgctacttca gctgcgcctt cgacggcgac
     1501 tgcgccgccg gccagcggtg caaggacgac aacttctgct cctgggactg agttcggcta
     1561 tcgcggccgc ggccgcgacg agtccgaggt tgtcgacgct tcggcctact cgatcgcggt
     1621 catacaggag agcgtgacct gcttcttcag cgcctcgcgt gccaggaaca accgcacgac
     1681 cagggtgttg cggttcttga cgggagcctt ggacgcatac tcctcggcgc ccacgctctc
     1741 gacctcggtg cgcttgagag ccacggcgta aacgggatcg aggttctcca cgagccggac
     1801 gaggctgcga tagacgacac gtcggagccc cgccgccgcc cgagaattcc cgtcgagatc
     1861 ggccgcgagc gccgacaatc cgcgactcgt gacctcatgt cgctcctgat aattgatcac
     1921 ggcaacgtgc aggtttcgca ggaaccacga gaccgcggag tcgtcgtcgc tcgggctgtc
     1981 cgtcccatcg ccgcagcgga cgaaggcgtc ttgcaggatg tcctcggcga gagcatggct
     2041 gctgacgcga gcttgcaaga gagccagaaa atcccggtga ctggcgacca ggcgttgatg
     2101 ggctgcagac gtgtcggcaa tccgccgcgt gtacctgtcg ccgtcggggt ggagcgtggg
     2161 tgccgtcatt tgaccccagc ctcgatcgcg gcaacgagga gcagaactac gcaggcacga
     2221 cgaacgagca tggaaggtct cccgcgctgg aggcgcacag gtcagacagg tcccgtccat
     2281 cccgttccaa tcgtgcggcg cgggtttgcg atgcgcagcc gtggcgactc gcgcgtgagg
     2341 gcgtgccagg cgatccagat cacgggccga gcggtgggtg ccgccggccc cagcagtatg
     2401 atgtcccctg ggcgtgcatc gggcgggttc gttatactgc cacggcgatg gcggccggga
     2461 gcgaggagac gaacgacgcc gcactccttg cggcggccca gcgaggcgag cgggctgcac
     2521 agacgcagct atataatcgc caccgcgagc gagtcgcccg gcaagtgctg cggatgatcg
     2581 ggaacagcgc cgccgtggat gacctcgtgc aggaggtgtt catccgcgcc ttcacggcgc
     2641 ttccccgatt tcggggtgat gcccagctga gcacctggct ctacacgatc gccaccaatc
     2701 gggtgcgcaa ctggtgggat tcacagcgcc gccagcagcg gcgcgaaacg atggcttctg
     2761 gcgatcctcc cgacgagtgc gaggatccga tggcccagat cgaagccaag gagcatctgg
     2821 cccgcttcta tatggcgctt ggggctttac cggacagatt gcgcgaggcg ttcgttgccc
     2881 gcgctatcga agggatgagc ctcctcgagg cctcggcagc tctgggacgg ccggtcagca
     2941 ccgtctcgta tcacacgctc cgggccgagg aggcgctctg cacggcgctc ggcatcgtct
     3001 acgtggagaa gtgacggtct ggcgcgccgc gcctcagtcc gtcacggcga cgacgaacct
     3061 tgccgccagc ggctcgggtc caggctcggc gcacagagcg agcgagtcct ctccagcgaa
     3121 cgacagctcg gctgctggtg tccaccccac aaacccgcgg ggagctccgc tctgaccacc
     3181 cagaaagttc atgagcagca gcctcggtgc gaggtcagcc ccgtagatag cgaggccata
     3241 tccatcgccc aggctttctt cgcagccggc gatcggtggt ttcgacgccc gaccgaaaac
     3301 atacaacaag tcaccaacgg ggcccccgag cgggctgtcg agcaggatcg gctggtggct
     3361 cttgagccgg tactgttgga ctggcgggcc atcgttcgtc agtctgtccg gcggatccca
     3421 cggacgcggc ttggtaccgc ggccataata gatgacatcg gctccggaga cgaccgcgac
     3481 gtgaacccgg tctcccgctg cgaacgcgca cttgctgaac cattcattat tgccgagatc
     3541 gatgccgtcg gtattttcga cgacgagaca ggcgttgaag gaacagtcct ccaggcacag
     3601 cggcactacc gtgctgtacg gctgggctgt gggatcgcag gcttcgtctg gatcgaccac
     3661 gccattaccg caagccgggt cgatctccca gcgacaggct gggctgcagc cgtcctcggc
     3721 gtcgaggttg ccatcgtcgc actcctcgcc gctggtgatc attccgtccc cgcagctctc
     3781 cagcatctcg aactggcagt ccccgtcaca gccgtccgcg gccgctcgat taccatcgtc
     3841 acaggtctct acaccaacct ggacgtgccc gtcaccgcag cgcgcgacgg tgcagttctc
     3901 gaggcaaccg tcgtcgttga cgaaattgcc gtcatcacat tgttcgacat cggcgaacaa
     3961 atggctgtca ccacagaacg cgtgcaggca gcgctccgta caggcatccc cgcccgcgat
     4021 attgccgtcg tcgcactgct cctccgccgt gataaccccg tcgccacagc tcggcgccgg
     4081 cggcgggagg gtgcaaatcg ctgtacagta cgtttcgtcc gcctcaccgt cgtcgcagac
     4141 ctcgccgctt tggaggatcc cgtccccgca caccacggtc ggtgccaggg cgaagaccgg
     4201 attatcgatc gcgcatgaga ctatcccgcc cagagccgcc agaacaaggc cgaccgtgcc
     4261 ccccgctccc cgcatgaacc gacggtaaca tgcccgtcgg cgcgattggg agcggagtga
     4321 accgctgagc cgcacgtccg cagagaaagt gatgcacagc acccgaggag tcacgcgcac
     4381 cagtccccag cgtgtccgcc tcagccggca ttgcaggtca gtgccacttg ccgtgcttgc
     4441 catgcttgcc gcccttccac ttgccgtgct tgcaatgacc gggcggccaa caaccatggt
     4501 catggtagcc atactcgacg ataatcaccg gcggcggcgc gacgtcatag acgacgactc
     4561 cataaaacgc cgggcggtgg acagcgtaga gccagccgta aaagccgccg cccccgctga
     4621 cgacgatgac gtcgtttttc acgacgatcg gcaccgtata gtcgtagacg atgacatcgt
     4681 agccgatcac caccggagcg taggtggcct tgacgatgag gacgttggtc gtcttgtccg
     4741 gcgtgaagtt gatgtaggcc aggacgatag cagcgtcctt gtccttggac ttcgagggga
     4801 tggcgtggag ctcgaagacg atgactccgt catctttctt gacctcgacg atgacgatct
     4861 tgtcgacctt gccgtcgccg tcgatatcta cgttgttgag cttctccttg gggttgttga
     4921 tcttcttctc gagcgcggcc gcgtccttga ccttgctctt cttgaccatg aaggtgatgc
     4981 tctcgagatc gagcgcctcc ggcttgatct cgacgtcgac cttggtcttg ccggcgggcg
     5041 gcgccttctc ctcggcgacc acgacggcga cctcggcgtc cacgacggct tcggcctcga
     5101 cggctggtgg cgccggggca tctccattgg cctgagaaac gatggccgcg tcacacgcgc
     5161 cggcaagcag ggagagggcg aggaagggga acagggcttt gacgtgcatg accgagcgtg
     5221 cattggacaa cctccgggcg ggagtttgca aggcatggca ttcgacgttt cagggggagc
     5281 acgccccctt tcggaccttc agcggggcgg tcgaggcgcc gttctcgagc ccatctgtga
     5341 cacgcggggc gaacccgtgc cgcacgttcc acggaaaatc gcgctgcgat gctcgcgcaa
     5401 ggtcctcaat gccgaccagc atggcgctct ccggccgccg aagcaccaga cgagccggtc
     5461 cctggatctt cggtcgatcc gaggcaacag aagaccatgc gctcctggat ttccctgctc
     5521 gccgcgctct ccggaggcct ggccctcaac tgcaagtcgc cggaacccac agataccgac
     5581 accggtagta gcggctccgg cagcacgtcg acctcggcgt cgacctctgc gccgacctcg
     5641 ctcgatccaa cgacgaccag cgccacgacc ggcgcgccga cgaccgggga cacgagcggc
     5701 gagcccgtga ctggcacggg cagcggctcg acgagttcga ccagcacctc ggacactaac
     5761 gacgcgacca cgaccaccgc gactaccggc ggttccagcg gcgacgcgac cacgatggac
     5821 ggcacgaccg gacccgtcgg gccgtgcaag ccgatcgcct gcgacggcaa gacctacgcc
     5881 tgcggtgact gcgaggacaa cgattcggac ggcaaggtcg acctggctga ctccgaatgc
     5941 atctcgccgt gcgacgaccg cgaggacacc ttcgccaccg gcctgccggg tgacaacatg
     6001 gacccctgca agcaggactg cttcttcgac ggtaactccg gcaacgacgc ctgcgactgg
     6061 aacctcgcct gcgacccgaa gaatcccggt ggcgatcagt gcccttacaa cccggacaag
     6121 gtgtgcgagc cgatgcagcc ggccgagtgc gccgagacct gtggcgtgcc caatggctgc
     6181 gactgcttcg gctgctgcac cgtcgtcgtc gagggcgtga gccacgacat ctttatcggt
     6241 gacaaggact gctctgttgc gaacatcgaa agctgccagc aatgcaccaa gaacgacgac
     6301 tgcgacgacg agtgcaagcc cgaagagtgc gaggtctgct tcggcggtga gttgccaccg
     6361 ggatgtgagg agccgacctg cgatcgcgcg aacccatgcc aactcgacaa tgagggcaat
     6421 tcggactgcc ctgaaggcac gttctgtcag accggctgct gcgagccgat tgccccgggc
     6481 tgacccaggt cttcgcgcca cgcgcgatcc cgtatccggc gtgccggcca cggagcgatc
     6541 gatgaatgtc atgcctgcga aatcactacc gctgcgacgt tgcctcctcg ccctgtcgtg
     6601 cctggcgatc ggcggttgcc tgttcaccga cccgggcccg gccgcaaacg agggcaacgc
     6661 ggaggcgagc ggggcgacct tcgacgctgg cggcgcagag gcgcagccca ccacggggcc
     6721 acaagcgacc accgaacccc aggccaccga cggcacgggc gagccgatgc tcgactgcaa
     6781 gtgggaacag gtcgtgacgt cgccgcgtcc cgcgaagcgc atcgacgctg ccctggcact
     6841 cgggcctgga ggggcgcagg tgctgctgta tggcgggcgc gtcgggttgc tcgggcccga
     6901 cctcgcggac acgtggacct tcgacggcga gcgctggcag ctcgtgaaca tcggcgaaga
     6961 gggcggcgga gggtggaagg agcagcccgg accgcgacgc ggacacgcga tggcctatga
     7021 cgcggcgcgc gaccaggtac tgctgttcgg cggcgagttc ggcggcgagt ccggcggcct
     7081 cgacgtcaag ttcgcggatc tcacttggat ccacgacggg gtgcaatgga gcgagcgcaa
     7141 gaatgggccg ttctctcgcg cctttgctgc aatggccgat ctgccgagta gcaaggtggt
     7201 ggtgctgttc ggcggtcgca cggacaaggg cgcgagcgcc gagacctgga tctggaacgg
     7261 tcaggtctgg gctaagcgag acttcgacgt cgctccatcg cc
//
LOCUS       DASWNJ010000740         2198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_296925, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000740 DASWNJ010000000
VERSION     DASWNJ010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296925"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VGB85_12410"
     CDS             <1..444
                     /locus_tag="VGB85_12410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0134884.1"
                     /translation="YVLTIARNKIADYFRKRKTSHTDEVLATSVRDLGAGPSSLLFKH
                     ENDRLLLEALRSIHLDDQLILELHYWDDMSGPELCQVFECTEPAIRSRIRRAKERLTS
                     ELQSLSRDPRELADTVTDLHSWARKLRDSLEPYLLQLKRNRPARN"
     gene            complement(494..>2198)
                     /locus_tag="VGB85_12415"
     CDS             complement(494..>2198)
                     /locus_tag="VGB85_12415"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEY0134885.1"
                     /translation="PKVHASRLRSERAALAASYPGMRELPAEVGAPTFEGKIAVDSSE
                     YWLRLTLPPGYPSVPPELREIDGPGGAVRIRPGTLFRFEDGSMCLFPHGRDPQAWHRD
                     RLAVEALDKFSALARDDQARAEGRGALLFRDSRTVYVAEAIAGWLRWPGGRGTLSLRV
                     APTGAGDLFADAVDIFGESAGATNVLGAAWSIALPLLCHVPWVRVPLAGQRWEDVAAT
                     RSHLDALLAAALPTAVFEQLRREESVVLVRAEEDADDPRLDAVLIGRPSAHLQALHLA
                     SVKVAHPQQRLFHRADGVLRSRTSLQGARVVIVGLGSLGGAVALALARAGIQRFVLID
                     PDRLSVDNICRHVGSISDLGRLKSEVIEEMLATINPEVEVTSIPKWLAWDLPWIGAGR
                     ELEQMLTETGRTVVIMTCAAHQAERQLNALAVESGVTVIYASALGAAEHGRIFRVIPQ
                     ETACYECILAAQEAEPGSFPRFIRGAPGGERVAYLQPGLPGLGIDITQIAMITSRFAL
                     QTIARLERMDLGFADEPGDHLLWTNRGGWIFDRPLQLMVEHIPRSPSCPVCGQGGHQA
                     E"
BASE COUNT          395 a          774 c          711 g          318 t
ORIGIN      
        1 tacgtgttga cgatcgcccg caacaagatc gcggactact ttcgcaagcg caagaccagc
       61 cacacggacg aggtcctcgc cacctcggtc cgcgatcttg gcgccggccc gtccagcctg
      121 ctcttcaagc acgagaacga ccgcctgctc ctcgaggcgc tccgcagcat tcacctcgac
      181 gatcagctga tcctcgagct gcattactgg gatgacatgt ccggccccga gctgtgccag
      241 gtgttcgagt gcacggagcc ggcgatccgg agccggatcc gtcgggccaa ggagcgcctc
      301 accagcgaat tgcagtccct ctcccgggat ccccgcgagc tcgccgacac cgtcaccgac
      361 ctccactcgt gggcgaggaa gctccgcgac agcctggagc cgtacctgct ccagctcaag
      421 cgtaaccggc cggcaagaaa ttgacggcgt cgacgccggc gagttcgacc cgtccccgcc
      481 tcgtcgacga cgctcactcc gcctggtgac cgccctgtcc gcagacgggg caggacggcg
      541 acctcggtat gtgctcgacc atgagctgga gcggccgatc gaagatccag ccgccgcggt
      601 tcgtccacag caggtgatcg ccgggctcgt ccgcgaaccc gaggtccatc cgttccaggc
      661 gagcgatcgt ctgcagggcg aagcggctgg tgatcatggc gatctgtgta atgtcgatgc
      721 cgagccccgg gaggcccggc tgcagatagg cgacacgctc acccccggga gcaccacgga
      781 taaaccgcgg aaagctcccc ggttcggcct cctgtgcggc caggatgcac tcataacagg
      841 cggtctcctg cgggatcacc cggaagatgc ggccgtgctc cgcggcgccc agggccgatg
      901 cgtagatcac cgtcaccccg ctctccaccg ccagcgcgtt gagctggcgc tccgcctgat
      961 gggcggcgca cgtcatgatg acgaccgtac gcccggtctc ggtgagcatc tgttccagct
     1021 cgcgaccggc gccgatccag gggagatccc aggcgagcca cttcgggatc gaagtcacct
     1081 cgacctcggg gttgatcgtg gcgagcatct cctcgatcac ctcgctcttg agccggccga
     1141 gatcgctgat cgagccgaca tgacggcaga tgttgtcgac cgagaggcgg tcggggtcga
     1201 tcaaaacgaa gcgctggatc ccggcccgtg cgagggcgag ggcgaccgcg cccccgagag
     1261 agcccagccc gacgatgacg acccgggccc cctgaaggct ggtgcgtgag cgcagcacgc
     1321 catcagcgcg gtgaaacaga cgctgctgcg gatgcgcgac cttcactgac gcgaggtgta
     1381 gcgcctgaag atgcgcggac ggccgaccga tgagcaccgc gtcgagccgc gggtcatccg
     1441 cgtcctcttc ggcgcgcacc aggaccacac tctcctcgcg ccgaagttgc tcgaagacag
     1501 ccgtcggcaa tgcagcggcg agtaatgcat cgaggtgcga gcgggtcgcg gcgacgtcct
     1561 cccagcgctg accggcgagc gggactcgca cccaggggac gtggcacaac agcggtagcg
     1621 cgatcgacca ggcggccccg aggacattcg tggcacctgc agactctccg aatatgtcca
     1681 ccgcgtccgc aaacagatcg cccgcccctg taggggcgac acggagactc aaagtcccgc
     1741 gcccgccggg ccatcgaagc cagcccgcga tcgcctccgc gacgtagacc gtccgtgagt
     1801 cgcggaacaa cagtgctccc cgcccctcgg cgcgtgcctg gtcatcacga gcgagcgcgg
     1861 agaacttgtc caacgcctcg actgccaaac gatctcggtg ccacgcctgc gggtcgcggc
     1921 cgtggggaaa caggcacatc gatccgtcct cgaagcggaa cagcgtgcca ggtcgtatac
     1981 gaaccgcgcc gccaggtcca tcgatctctc gcagctcggg agggaccgac gggtagcccg
     2041 gaggcaaggt caatcgcagc caatactcgg acgagtcgac ggcgatcttg ccctcgaagg
     2101 tcggcgcgcc gacttcggcc ggaagctctc tcatccccgg gtaactggcc gccagagcgg
     2161 ctcgctcgct gcggaggcgg gacgcgtgaa ccttcgga
//
LOCUS       DASWNJ010000741         2452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_578919, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000741 DASWNJ010000000
VERSION     DASWNJ010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578919"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VGB85_12420"
     CDS             <1..488
                     /locus_tag="VGB85_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134886.1"
                     /translation="LGDDDSGETPKQRAAATAAGGDAPALASAPTTGLPSRRELVAEV
                     PEPYAKHVQVLLLSTDPDERQRAGEAIAGAPEADKAAIPEYLRNIAWLDKVSACDTKR
                     SILLKIDAADDLRALWALRILDATPRDGCRRIFGIRDDCLGCLREDLARMLPRFEARA
                     E"
     gene            complement(515..1006)
                     /locus_tag="VGB85_12425"
     CDS             complement(515..1006)
                     /locus_tag="VGB85_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134887.1"
                     /translation="MFRRAWLSFVLAACGAEPAVTGAPQTVFEERAGPSSAAPTCAEG
                     DGSGVCPQVNASPAAKQPDFMQAASVCRQAVLDEQSKCAVHWTQACDRALLAAAHRKS
                     EKPARTGACYYHDRKTCPDCACEYYLKVGKIGFDGGTGCLGSLDDVTRELRRACAEQR
                     CEP"
     gene            complement(1073..>2452)
                     /gene="lnt"
                     /locus_tag="VGB85_12430"
     CDS             complement(1073..>2452)
                     /gene="lnt"
                     /locus_tag="VGB85_12430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEY0134888.1"
                     /translation="VMGAVGFPWMIGMMQTFAELPLWIALPLFALFCLWTAVPLGIWG
                     ALCGRGGAAPWVAVCFTGIWWSWPAVFPFTVMLGLAAQPVWIQSAELGGVALVEVLVL
                     LCNVLIADGLARRRPGHLALALAIPALCFVLGTMRMRALAGETTRVVRVGIVQPNIPL
                     LWSGPEAKQAKLARLREPSARAQAEGAELIVWPENMYPWPLDRPIRRDFTDDDRILAL
                     HSLPTIFGAGSIADEDPFGYNTVFNMAADGEVLATFDKVLLVPLGEHIPIVDPVWATS
                     QVAGMAHNFAGAGPVRFVVTPGPVGSTAPPIAVGPLVCYEDVAAGFAREVAAQPGGIS
                     LFVNLTNDTWFGAGAEPWGHLALAQFRSVEHRIPMVRSVNSGPSSAIDRAGRVTATTT
                     VRPAEVAALVPPEYVVAEVAIGRDTEGAPTVFARGGWLLAHLCQCIAAVSLVLVVLRR
                     HRARRGERA"
BASE COUNT          376 a          947 c          844 g          285 t
ORIGIN      
        1 cgctcggcga cgacgactcc ggcgagaccc cgaagcagcg cgccgcggcg acggcggccg
       61 gtggtgacgc gccggccctc gcgtccgcgc cgacgaccgg cctgccctcg cgccgcgagc
      121 tggtcgccga ggtcccggag ccctacgcca agcacgtgca ggtgctgctg ctgagcaccg
      181 accccgacga gcgccagcgc gccggcgagg cgatcgccgg ggcgcccgag gccgacaagg
      241 ccgcgatccc ggagtacctg cgcaacatcg cctggctcga caaggtctcc gcgtgtgaca
      301 ccaagcgatc gatcttgctc aagatcgacg cggccgacga cctgcgcgcc ctgtgggccc
      361 tgcggatcct cgacgccacc ccgcgcgacg gctgccgccg gatcttcggc atccgcgacg
      421 actgcctcgg gtgcctgcgc gaggacctcg cgcgcatgct gccgcgcttc gaggcgcgcg
      481 ccgagtgacc tgaccttccg gacaggtcac tcgctcacgg ctcgcagcgc tgctcggcgc
      541 aggcgcgtcg cagctcgcgg gtgacatcgt cgaggctgcc gaggcagccg gtgccgccgt
      601 cgaagccgat cttgccgacc ttcaaataat actcgcaggc gcagtccggg caggtcttgc
      661 ggtcgtggta gtagcaggcg ccggtgcgcg ccggcttctc cgacttgcga tgggccgcgg
      721 cgagcaaggc ccggtcacac gcctgcgtcc agtgcacggc gcacttcgac tgctcgtcga
      781 gcaccgcctg acggcacacg ctcgccgcct gcatgaagtc gggctgcttc gccgccggtg
      841 acgcgttcac ctgcgggcac accccggagc catcgccctc cgcgcaggtg ggggccgcgg
      901 acgacggccc ggctcgctcc tcaaacaccg tttgcggggc gcctgtcacc gccggctcgg
      961 cgccacaggc ggcgagcacg aacgacagcc acgcgcgacg aaacacccgc gcagcatctc
     1021 cgggtccgtc cgcccgcgca agccggcgat cgacgggcgc gtggacgcac gctcacgcac
     1081 gctcgccgcg gcgggcccgg tggcggcgca gcaccacgag gacgagcgac accgcggcga
     1141 tacactggca caggtgcgcc aggagccagc cgccgcgcgc aaacacggtc ggcgcgcctt
     1201 ccgtgtcgcg accgatcgcc acctcggcca ccacgtactc cgggggcacg agcgcggcga
     1261 cctcggccgg gcgcacggtc gtggtggcgg tgactcgacc ggcgcggtcg atcgccgagc
     1321 tcggccccga gttgacgctg cgcaccatcg ggatccggtg ctcgacgctg cgaaactgcg
     1381 cgagcgcgag gtgcccccac ggctccgcgc cggcgccgaa ccacgtgtcg ttggtcaggt
     1441 tgacgaacag cgagatgccg ccgggctgcg ccgccacttc gcgggcgaag cccgccgcga
     1501 cgtcctcgta acagaccagc ggcccgaccg cgatcggcgg cgccgtgcta ccgaccggcc
     1561 ccggcgtgac gacgaagcgc accggccctg ccccggcgaa gttgtgggcc atgcccgcga
     1621 cctgcgaggt cgcccacacc gggtcgacga tcgggatgtg ctcgccgagc ggcaccagca
     1681 gcaccttatc aaaagtcgcc agcacctcgc cgtcggcggc catgttgaac acggtgttgt
     1741 agccgaaggg gtcctcgtcg gcgatgctgc ccgcgccgaa gatcgtcggc agcgagtgca
     1801 gcgcgaggat ccggtcgtcg tcggtgaagt cgcgccggat cggccggtcg agcggccacg
     1861 ggtacatgtt ctccggccac acgatgagct cggccccctc ggcctgggcc cgcgccgagg
     1921 gctcacgcag gcgcgcgagc ttggcctgct tggcctcggg gccgctccac agcagcggga
     1981 tgttcggctg cacgatcccg acacggacca cgcgcgtcgt ctcgccggcg agcgcgcgca
     2041 tgcgcatggt cccgaggaca aagcacagcg ccgggatcgc cagcgccagc gccaggtgtc
     2101 ccggcctgcg ccgcgccagc ccgtcggcga tcagcacatt gcacagcagc acgagcacct
     2161 cgacgagcgc gaccccgccg agctcggccg actggatcca caccggctgg gccgcgagcc
     2221 cgagcatcac cgtgaacggg aacaccgccg gccacgacca ccatatgccg gtgaaacaca
     2281 ccgcgaccca cggggccgcc ccgccgcgcc cgcacagcgc cccccagatc ccgagcggca
     2341 cggccgtcca caggcagaac agggcgaaca gcggcagcgc gatccacagc ggcagctcgg
     2401 cgaaggtctg catcatcccg atcatccacg ggaagccgac cgcgcccatc ac
//
LOCUS       DASWNJ010000742         2244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_683589, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000742 DASWNJ010000000
VERSION     DASWNJ010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683589"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VGB85_12435"
     CDS             complement(<1..506)
                     /locus_tag="VGB85_12435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0134889.1"
                     /translation="MSTIAAFGSALSRIFDTSEAAADSGRAQALVLDLFDRHVRDARL
                     GLQIGGRSFTVGQGPGEPLATLRLRDASVFTRILAAGNLGLGEAYMDGAVECVGGELH
                     EFLTALLRNRVDQALQSDPRLLARAAWVRLQTLARSKAENVRRHYDAGDDLFAAFLDP
                     TMTYSCGY"
     gene            660..1793
                     /locus_tag="VGB85_12440"
     CDS             660..1793
                     /locus_tag="VGB85_12440"
                     /inference="COORDINATES: protein motif:HMM:NF015254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectin acetylesterase-family hydrolase"
                     /protein_id="HEY0134890.1"
                     /translation="MPAASPRVLIWLLPCPLVLATACGDGGEASTNSTSTTDDDGGDP
                     PTTGSACGGDVIDAPPGQWTWVPVEGARCGRGSPTGMAINPGGDRLLIYMLFGGACST
                     AEECAPDCDPQAQRCALHLDGFDETTFKDTLGLFGAGSIFDREDPGNPYRDHSFAFIP
                     YCTGDFHSGDREAEYGISHVGYRNMGLYLERLVPSFCGATDVVLVGSSAGGFGAVFNY
                     DRVREAFAPRRVDLVDDAGPPLGPEWMPIQTQMRAAWGSDLTAPAGCPACASRWDAFL
                     PYLGATAPEARLALISSLADTSIGPFFGGPIALPADYTAAMNEYADLTLGPVDRVEVF
                     YLDEPHHVYLVDDLAQTGPPAVSLQTWLTQMHSDDPAWASVRP"
     gene            complement(1807..>2244)
                     /locus_tag="VGB85_12445"
     CDS             complement(1807..>2244)
                     /locus_tag="VGB85_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134891.1"
                     /translation="RDSALVQVDTIEAYASSLENSATAPHDAATALQAKATELRANAS
                     PAGETEEKAAAELLRAAQRWRAAADAWALAAQQLSPYWDPDGRGFKAFARELRALDQA
                     RSRGARIVNSLTLAHKDYDALGGTDHRERIAAAIVLGRQLAAG"
BASE COUNT          293 a          789 c          832 g          330 t
ORIGIN      
        1 atgtagccgc aggagtaggt catcgtcggg tcgaggaacg ccgcgaacag gtcgtcgccg
       61 gcgtcgtagt ggcggcgcac gttctcggcc ttgctgcgcg ccagcgtctg cagccgcacc
      121 caggcggcgc gggcgagcag acgcgggtcg ctctgcaacg cctggtcgac gcggttgcgc
      181 agcagggcgg tcaggaattc gtgcagctcg cccccgacgc actcgacggc gccgtccatg
      241 tacgcctcgc cgagcccgag gttgccggcg gcgaggatgc gggtgaacac gctggcgtcg
      301 cgcaggcgca gggtcgccag cggctcgccc ggcccctgcc cgaccgtgaa gctgcgcccg
      361 ccgatctgga gaccgaggcg agcgtcgcgg acgtggcggt cgaacaggtc gagcacgagc
      421 gcctgggccc ggccggagtc cgcggcggcc tcgctcgtgt cgaagatgcg cgagagcgcg
      481 gaaccaaacg cggcgatcgt actcatgtcg ctgtccaggg tgtcgggccg tcggtgcggg
      541 gtccgagagt aagccgctcg cgggctcgcg gcggatcact cgccggctgc gaccgacgcg
      601 cgacgagcga accagcggcg cgatttcgct ggttcgctgg tttgcgggta tcaaggtgca
      661 tgcccgccgc gtcgccccgc gtcctgatct ggctcctccc gtgcccgctg gtcctggcga
      721 ccgcgtgcgg cgacgggggc gaggcatcga cgaacagcac gtcgaccacg gacgacgacg
      781 gtggtgatcc gcccaccacc ggatcggcgt gcggcggtga tgtgatcgac gcaccgccgg
      841 ggcagtggac gtgggtgccg gtcgaggggg cgcgctgcgg ccgtggcagc ccgacaggca
      901 tggcgatcaa cccgggcggc gatcgcctgc tcatctacat gctgttcggc ggcgcctgct
      961 ccaccgcgga ggagtgcgcg cccgactgcg atccgcaggc acagcgctgc gccctccacc
     1021 tcgacggctt cgacgagaca acttttaaag atacgctcgg gctgtttggg gcgggcagca
     1081 tcttcgaccg cgaggacccg ggcaacccgt accgcgacca cagcttcgcg ttcattccct
     1141 actgcaccgg cgacttccac agcggcgacc gcgaggccga gtatggcatc agccacgtcg
     1201 gttatcgcaa catgggtctg tacctcgagc gcctggtgcc gagcttttgt ggggcgaccg
     1261 acgtggtgct cgtcggctcg agcgcgggcg gcttcggtgc ggtcttcaat tatgatcgcg
     1321 tgcgcgaggc cttcgcgccg cgtcgcgtcg acctcgtcga cgacgccgga ccgccactgg
     1381 ggcccgagtg gatgccgatc cagacgcaga tgcgcgcggc gtggggctcc gacctgaccg
     1441 cgccggccgg ctgccccgcg tgcgcgagcc gctgggacgc cttcctgccg tacctcggcg
     1501 cgacggcccc ggaggcgcgt ttggccctga tcagcagcct cgccgacacc tcgatcgggc
     1561 cgttcttcgg cgggccgatc gcgctgccgg ccgactacac cgcggcgatg aacgagtacg
     1621 ccgatctcac gctggggccg gtcgaccgcg tcgaggtgtt ctacctcgac gagccgcacc
     1681 acgtgtatct cgtcgacgac ctcgcgcaga ccggaccgcc ggcggtgtcg ctgcagacct
     1741 ggctgacgca gatgcacagc gacgaccccg cgtgggccag cgtgcggccc tgaggacgac
     1801 gcggcctcag ccggcggcga gctgacggcc gaggacgatc gccgcggcga tgcgctcgcg
     1861 gtggtcggtg ccgccgagcg cgtcgtaatc cttgtgggcg agcgtcagcg agttgacgat
     1921 ccgcgcgccg cggctgcgcg cctgatcgag ggcccgcagc tcgcgggcga aggccttgaa
     1981 gccgcggccg tccgggtccc agtacggcga gagctgctga gccgcgagcg cccacgcgtc
     2041 ggcggccgcc cgccaccgct gggccgcgcg cagcagctcg gcggcggcct tctcctcggt
     2101 ctcgccggcc ggcgaggcgt tggcccgcag ctcggtcgcc ttcgcctgca gcgcggtggc
     2161 ggcgtcgtgc ggggcggtcg cgctgttctc gaggctgctt gcataggcct cgatcgtgtc
     2221 gacctggacc agcgcgctgt cgcg
//
LOCUS       DASWNJ010000743         1557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_398158, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000743 DASWNJ010000000
VERSION     DASWNJ010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398158"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VGB85_12450"
     CDS             complement(<1..612)
                     /locus_tag="VGB85_12450"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY0134892.1"
                     /translation="MRAPLPANENERLEALYRCGILDTEPEPSFDEIASLAGQLCATP
                     MALVSLVDADRQWFKARVGLPATETPRDVSFCAHAVASNEPLVVDDTLQDTRFAGNPL
                     VTSDPNIRFYAGVPLTLEDGLTVGTLCVLDRVPRRLSPAQMAALGMLARQVTTELGLR
                     RALSSAQLQRQAEAQLASSRATPSYAGVPVPRVGEPELPRQIRN"
     gene            764..1510
                     /locus_tag="VGB85_12455"
     CDS             764..1510
                     /locus_tag="VGB85_12455"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY0134893.1"
                     /translation="MHSRHVLLESPALGRQVHLWCHGQFGPPVVVFPSAAGMAHEWDR
                     HGMLEVLAPLINGGRVKLYCPESNVSETWTAKDTPLAHRMRRHAAYERFVLETLVPFV
                     REDCRWPTAPITAMGCSLGGTYSALFALKFPETFKQALCMSGRYLTTELTGGENSQDL
                     YFNNPLAFVTGMHGEALERVRRNTHLTLVCGRGKWEEGCIEETIALGKLLRDKQIPSE
                     TDIWGPESRHDWDYWQKQVVMHLPRVLARL"
BASE COUNT          242 a          550 c          537 g          228 t
ORIGIN      
        1 gttgcggatc tgtcgcggca gctcgggctc gccgacccgc ggcaccggca cgcctgcata
       61 cgacggcgtc gcccgcgacg acgccagctg ggcctccgcc tggcgctgga gctgggccga
      121 ggacagcgcg cgacgcaggc cgagctcggt cgtcacctgg cgcgccagca tgccgagcgc
      181 ggccatctgc gcggggctca gccgccgcgg cacccggtcg agcacacaca gcgtcccgac
      241 cgtcaggccg tcctcgagcg tcagcggcac gccggcataa aagcgaatgt tcgggtcgga
      301 ggtgacgagc ggattcccgg cgaagcgggt gtcctgcagc gtgtcgtcga cgaccagcgg
      361 ctcgttgctg gcgaccgcgt gggcgcagaa cgacacgtcg cgcggggtct cggtcgccgg
      421 caggccgacc ctcgccttga accactggcg gtcggcgtcg accaggctga cgagcgccat
      481 cggggtggcg cacagctgcc ccgcgaggct ggcgatctcg tcgaagctcg gctcgggctc
      541 ggtgtcgagg atgccacagc gataaagcgc ctcgaggcgc tcgttttcgt tcgcggggag
      601 cggtgcacgc atgggggacg cctggcctcc cgcctgcatc ggggaaccgt ccagttaatg
      661 cgcaacgtgg ttcggttcgt caacccgcgg gagcgccgcg gtcccctcgc gggtacacga
      721 cttgttggac acaggggcgg ggtcgagcgc atattgcggc ggcatgcatt cgcgccacgt
      781 cttgctcgag agccccgccc tggggcgcca ggtccacctg tggtgtcacg gccaattcgg
      841 gccgccggtg gtcgtatttc cctccgccgc cggcatggcc cacgagtggg accgccacgg
      901 catgctcgag gtgctcgcgc cgctgatcaa tggcggccgc gtcaagctgt actgccccga
      961 gagcaacgtc tccgagacct ggaccgccaa ggacaccccg ctcgcccacc gcatgcggcg
     1021 ccacgccgct tacgagcgct tcgtgctcga gacgctggtg cccttcgtgc gcgaggactg
     1081 ccgctggccg accgcgccga tcaccgcgat gggttgcagc ctcggcggca cctactcggc
     1141 cctgttcgcc ctgaagttcc ccgagacctt caagcaagcg ctgtgcatga gcggccgcta
     1201 cctgacgacc gagctgaccg gcggcgagaa cagccaggac ctgtacttca ataatccgct
     1261 cgccttcgtc accggcatgc acggcgaggc gctggagaga gtccgtcgca acacccactt
     1321 gacgctggta tgcggccgcg gcaaatggga agagggctgc atcgaggaga cgatcgccct
     1381 cggcaagctc ctgcgcgaca agcagatccc cagcgagacc gacatctggg gcccggagtc
     1441 ccgccacgac tgggactact ggcaaaagca ggtcgtcatg cacctgcccc gcgtgctcgc
     1501 ccgcctgtga cccatgaccg aagatagccg cgcgcggtgg ctcgcctgga agacggc
//
LOCUS       DASWNJ010000744         2927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_154979, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000744 DASWNJ010000000
VERSION     DASWNJ010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154979"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VGB85_12460"
     CDS             <1..454
                     /locus_tag="VGB85_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134894.1"
                     /translation="EAGFSMMAPIQRLTKWLEVFRRIDDPEFVTTFLAMEAWASDNVP
                     FPGELYRQYIKDCYQENNFCNGRMIVGGRKVDLSQITCPLLNLIADNDSIAPPPSSTI
                     LNEKVSSTDKTTLRFPVGHIGLSTSSKGPKQIWPKVASWLAQHSEPVK"
     gene            complement(464..1507)
                     /locus_tag="VGB85_12465"
     CDS             complement(464..1507)
                     /locus_tag="VGB85_12465"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0134895.1"
                     /translation="MPTSQLVPTRRLAIHTLTAGAERGPAVVLIHGNVSTSAFWEPLV
                     DAIDGRFRVIAPDLRGYGATEAAPTDATRGVRDFSDDLHALLETLEVTQPVHLVGWSA
                     GAGVAMQFAIDHPTRVAGLVLESPVSPFGFGGTRDAAGRPCHADFAGSGGGTANPDFV
                     KLLRAGERGSEGPVAPRNVMNTCYYRPPFRTDAALEERYLDAMLTTRIGDDQYPGDLT
                     TSPNWPGVAPGTRGMNNALSPKYLDLSSFAAIDPRPPVLWIRGADEAIGSDTSLFDLG
                     FLGKLGLVPGWPGDEAYPAQPMVAQMRGVLETYRARGGRYDELVLGACGHSPHIERQA
                     AFASALNNFLNGA"
     gene            complement(1593..>2927)
                     /locus_tag="VGB85_12470"
     CDS             complement(1593..>2927)
                     /locus_tag="VGB85_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134896.1"
                     /translation="DDPSMLKAFQAIDTIMTQRKDWKALERAYRKMINRLPADADPAL
                     RMMLWTNLAEIYRSRLVDFRAAAKAFEVAAALDPNNLDRYLLLAELYEMLVRNDPREF
                     TDHLVRTHFTLLRLEPSRYASYHRLFDIYLARRELDKAFCVARTLTFLKQASAQQTTL
                     VNRYPQPEFRRIKQKLGEDVLRRHVFHPDEDPTIGAILGLVAPVLSAWRVKQLPYPLR
                     PNDRVDPDDDPSLVGKMLLYVIGVLGLGQPDLYFRPDEPGDLHVLNVQREGRVRPTMI
                     ALANAIRDKSENNLVFAVGRHALDLYPPHYGFMVIDRSPENLKDVIYACMLVSGVKPL
                     EPSPGVAACARELRQHLTPPIIDQLAGLLRRLVQAERIDVKGWARAAELAGYRVGFLM
                     CNDLATAAQAISREQRELGSFLTPKDALRELVVYSVSEDYFQARRALGLNVA"
BASE COUNT          445 a          931 c         1104 g          447 t
ORIGIN      
        1 ggaggccggc ttctcgatga tggccccgat ccagcgcctc accaagtggc tggaggtgtt
       61 ccgccgcatc gacgaccccg agttcgtgac gaccttcctg gcgatggagg cgtgggcgtc
      121 ggacaacgtg cccttccccg gcgagctgta ccggcaatat attaaggatt gttatcagga
      181 gaacaatttt tgtaacggcc gcatgatcgt cggcgggcgc aaagtcgacc tgtcgcagat
      241 cacgtgcccg ttgttgaacc tgatcgccga caacgactcg atcgccccgc caccctcgag
      301 cacgatcttg aacgagaagg tcagctcgac cgacaagacc accctgcgct tcccggtcgg
      361 ccacatcggc ctgtcgacgt cgagcaaggg accgaagcag atctggccga aagtggcctc
      421 gtggctcgcg cagcactccg agccggtcaa gtaggcgcct ggctcaggcg ccgttgagaa
      481 aattgttgag cgcgctggcg aaggcggcct ggcgctcgat gtgcggcgag tggccgcacg
      541 cgccgaggac cagctcgtcg tagcggccgc cgcgggcgcg gtaggtctcg agcacgccgc
      601 gcatctgggc gaccatcggc tgggccgggt aggcctcgtc accgggccag ccgggcacga
      661 ggccgagctt gccgaggaag ccgaggtcga acaggctggt atcgctgccg atcgcctcgt
      721 cggcgccgcg gatccacagc accggcgggc gcgggtcgat cgcggcgaag ctcgacaggt
      781 cgaggtactt cggcgacagc gcgttgttca tgccgcgcgt gccaggagcg acgccgggcc
      841 aattcggcga ggtcgtcagg tcgccggggt actggtcgtc gccgatgcgg gtcgtcagca
      901 tcgcgtcgag gtagcgctcc tcgagcgcgg cgtcggtgcg aaacggcggc cggtagtagc
      961 aggtgttcat cacgttgcgc ggggcgaccg ggccctcgct gccgcgctcg ccggccctga
     1021 gcagcttgac gaagtcgggg ttggccgtgc cgccgccaga gccggcgaag tcggcatggc
     1081 agggccgacc ggcggcgtcg cgcgtgccgc cgaagccgaa gggcgacacg gggctctcga
     1141 gcacgagtcc ggcgacacgc gtcgggtggt cgatcgcaaa ctgcatcgcg acgccggcgc
     1201 cggccgacca tccgaccagg tgcaccggct gcgtcacctc gagcgtctcc agcaacgcgt
     1261 gcaggtcgtc gctgaagtcg cggaccccgc gggtcgcgtc ggtcggggcg gcctcggtgg
     1321 cgccgtagcc tcgcaggtcg ggggcgatca cgcgaaagcg gccgtcgatc gcgtcgacga
     1381 gcggctccca gaacgcgctg gtcgagacgt tgccgtggat cagcacgacg gccggcccgc
     1441 gctcggcacc ggcggtgagg gtgtggatcg cgaggcgcct ggtcggaacg agctgcgagg
     1501 tgggcatgag agaccggcga gcgtcgcaca ggcacgcggc gctcgccagg gtcacggcgc
     1561 gcagacgctg cgcgtgcgac cgtcagggcc gatcaggcga cgttgaggcc gagcgcgcgg
     1621 cgggcctgga agtagtcctc gctgacgctg tagacgacga gctcgcgcag ggcgtccttg
     1681 ggggtcagga acgagccgag ctcgcgctgc tccctcgaga tcgcctgcgc cgcggtggcg
     1741 aggtcgttgc acatcaggaa gccgacgcgg taaccggcca gctcggcggc gcgggcccag
     1801 cccttgacgt cgatgcgctc ggcctggacc agcctgcgca acaaccccgc cagctggtcg
     1861 atgatcggcg gcgtcaggtg ctggcgcagc tcacgggcgc acgcggcgac gccgggcgat
     1921 ggttcgagcg gcttgacccc cgagaccagc atgcacgcgt agatgacgtc cttgaggttc
     1981 tccggcgagc ggtcgatcac catgaagccg tagtgcggcg ggtagaggtc gagcgcgtgg
     2041 cggccgaccg cgaacacgag gttgttctcg ctcttgtcgc ggatcgcgtt ggcgagcgcg
     2101 atcatcgtcg ggcgcacgcg cccctcgcgt tgcacgttga gcacgtgcag gtcgccgggc
     2161 tcgtctggcc gaaagtacag gtcgggctgg ccgaggccga gcacgccgat gacgtagagc
     2221 agcatcttgc cgaccagcga ggggtcgtcg tcggggtcga cgcggtcgtt cgggcgcagc
     2281 gggtacggca gttgtttcac gcgccaggcc gacagcaccg gcgcgaccag cccgaggatc
     2341 gcgccgatcg tcgggtcctc gtcggggtgg aacacgtgcc tgcgcaggac gtcctcgccg
     2401 agcttctgct tgatccggcg gaactccggc tgcgggtagc ggttgaccag cgtggtctgc
     2461 tgcgcgctcg cctgcttgag gaaggtcagc gtgcgggcga cgcagaacgc cttgtcgagc
     2521 tcgcggcgcg cgaggtagat gtcgaacagg cggtggtagc tggcgtagcg cgagggctcg
     2581 aggcgcagca gggtgaagtg cgtgcggacg aggtggtcgg tgaactcgcg cgggtcgttg
     2641 cgtaccagca tctcgtacag ctcggccagc aacaggtagc ggtccaggtt gttggggtcg
     2701 agcgcggcgg cgacctcgaa ggccttggcg gcggcgcgga agtcgaccag gcgcgagcga
     2761 tagatctcgg cgaggttggt ccacagcatc atgcgcagcg ccgggtcggc gtcggccggc
     2821 aggcggttga tcatcttgcg gtaggcccgc tcgagcgcct tccagtcctt gcgctgggtc
     2881 atgatcgtgt cgatcgcctg gaacgccttg agcatgctcg ggtcgtc
//
LOCUS       DASWNJ010000745         3539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_439033, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000745 DASWNJ010000000
VERSION     DASWNJ010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439033"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1116
                     /locus_tag="VGB85_12475"
     CDS             169..1116
                     /locus_tag="VGB85_12475"
                     /inference="COORDINATES: protein motif:HMM:NF020968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HEY0134897.1"
                     /translation="MMRHRGFPKIAGPGESPGAAPPGGPWVALEKIHGAQMVVAIAGD
                     ELQFGKRKAWLRHDDPFFGWQLLRAPLTLAAQAAKAHLTVHTVVLYGELYGGAYPHPD
                     VPAVPGLAPVQTGIWYAPDLRYAVFDVLVADGEHDEGEFLAHREVTALARAVGLDSPP
                     VLHSGPRSAALMVPVRQPTRVPVALGLPELPGNFAEGVVLKADARARPSSRIVYKQKI
                     AEFDEQRFGESEAWDADQRLDLAALLAHARRLVNPPRIASAASKWGLDDRPALLDEVA
                     LDVMIDLAAAFPTAVAALPPADEDRLRAAILAAAEAALT"
     gene            1329..2432
                     /locus_tag="VGB85_12480"
     CDS             1329..2432
                     /locus_tag="VGB85_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134898.1"
                     /translation="MRLRLLIIFMFLLACQHDGGAPGGVVDLGTTSGTSSSSGDPPPT
                     TDAHPTGTTGTPTSTTTGSTSAGDETTAPASTSSETSEPSPTCGDGHVDPTEQCDLGP
                     DNSDHGACTVTCKAATCGDGLVWEGTETCDHAGNNNDTLYGACTTQCQWGARCQDGII
                     QGPEECDLGDANNTGESPPNGVPCDGCRFQARIAFLSSVAYKGGELGGVEGAHLRCQA
                     LAQQSGLDNSASFMAWLSDAQHSPLQDFKNGPDTAGLPYVRPDGIRIADDWNDLLLNG
                     PGDGITVTETGELQLIKGVWTGTAPSGGVFDPAATCKAWSSSKAIDQSRIGTSGVDEQ
                     QVDAWMQWVSFKQWSSFKSIACQNTYQIYCFEQ"
     gene            2447..3334
                     /locus_tag="VGB85_12485"
     CDS             2447..3334
                     /locus_tag="VGB85_12485"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0134899.1"
                     /translation="MKHRTRLLDRVSRVCAVVAGSVLTSACFVSDIEAIIADYEVTEA
                     PGSSDEGSSSQDASTGTSTGMTADSTGGPGTSTSGVDETTSTGSGSGDSSSTGTTGPV
                     CGDGLVEGDETCDDGNDIPGDGCQSCAADSIVFITSERYQGFKLGGLYGADQRCRNLA
                     AKAGLARPLTFMAWLSTSTMSVADRMLHSRGRYVLVNGLVVAQDWDALTSGTLETPIA
                     VTEYSETREDLVWTGTLPGGQPSLGSDFCGDWDDPSAFNEDGGSGYSLATDAWWSFFD
                     HNGCISELPLYCVEQREAP"
     gene            3331..>3539
                     /locus_tag="VGB85_12490"
     CDS             3331..>3539
                     /locus_tag="VGB85_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134900.1"
                     /translation="MIGPLPSVLSRVALASLLVTPACNGPTSEASSTGTTQETSSTAS
                     DATPGTSTTTAPASTSTQTGGLDTST"
BASE COUNT          584 a         1362 c         1094 g          499 t
ORIGIN      
        1 cgcgcgctgg cgatgaccgc gatccagtcc gacccggagc tgtccgccga catcgcccgt
       61 cacaattttg aaaacgtcgg cgccggcccg ggccgcgaga ccctcgagtc ccgcatcgcc
      121 gagctgctgc tcgcgcgcgc cgccctttaa ttattaaaat accctgacat gatgcgccac
      181 cgaggcttcc cgaagatcgc cggacccggc gagagccccg gcgccgcccc gcccggcgga
      241 ccatgggtcg cgctcgagaa gatccacggc gcccagatgg tcgtcgccat cgccggcgac
      301 gagctgcagt tcggcaagcg caaggcctgg ctgcgccacg acgacccctt cttcggctgg
      361 cagctgctgc gcgccccgct gacgctcgcc gcacaggccg cgaaggcgca cctcaccgtg
      421 cataccgtgg tcctctacgg cgagctgtat ggcggcgcct atccccaccc cgacgtgccc
      481 gcggtgcccg gcctcgcgcc ggtgcagacc ggcatctggt acgcgccgga cctgcgctac
      541 gccgtgttcg acgtgctcgt cgccgacggt gagcacgacg agggcgagtt cctcgcgcac
      601 cgcgaggtga ccgcgctggc ccgcgcggtc ggtctcgaca gcccgccggt gctgcacagc
      661 ggcccgcgct ccgcggcgtt gatggtgccg gtgcgccagc cgacgcgcgt cccggtcgcg
      721 ctcggcctgc ccgagctgcc cggcaacttc gccgagggcg tggtgctcaa ggccgacgcc
      781 cgcgcgcgcc cgtcgagccg gatcgtttac aagcagaaga tcgccgagtt cgacgagcag
      841 cgcttcggcg agagcgaggc ctgggacgcc gatcagcggc tcgacctcgc cgccttgctg
      901 gcccacgcgc gccgcctcgt caacccgccg cggatcgcca gcgccgcctc gaagtggggc
      961 cttgatgatc gccccgcgct gctcgacgag gtcgccctcg acgtcatgat cgacctcgcc
     1021 gccgcgttcc cgaccgccgt cgcagccctg ccgccggccg acgaggaccg cctgcgcgcg
     1081 gcgatcctcg ccgccgccga ggcagcgttg acctgagaaa caccggtccg cgccgccccc
     1141 cgccggccct tgacacagcc cgtgaccccg ggcaacgtgg cgacgcgcga tttcacgccc
     1201 ccggccatgc tgccgatcct ggcgccgatc ctggcgctcg cgccccagac gatcgtcctg
     1261 tcccggaggc cgccgaacag ctcggtcctc gggcttgaaa agacttcctc acgctcccct
     1321 gagaccccat gagacttcgc ctgctcatca tcttcatgtt tctcctcgcc tgccagcacg
     1381 acggtggagc gcccggcggc gtcgtcgacc tcggcaccac gagcgggacc tcatcgtcct
     1441 cgggcgaccc acccccgacg accgatgccc acccgaccgg cacgaccggc accccgacga
     1501 gcacgaccac cggttcgacc tcggccggcg acgagaccac cgcccccgcg agcacctcga
     1561 gcgagacctc cgagccgtcc cccacctgcg gcgacggtca cgtcgacccc accgagcagt
     1621 gcgacctcgg ccccgacaac agcgaccacg gcgcctgcac cgtgacctgc aaagcagcga
     1681 cctgcggcga cggcctggtg tgggagggca ccgaaacctg tgaccatgcc ggcaacaata
     1741 acgacacgct ttacggtgca tgcaccaccc agtgccagtg gggcgccaga tgccaggacg
     1801 gcatcattca gggccccgag gagtgcgacc tcggtgacgc caacaacacc ggcgagtccc
     1861 caccgaacgg cgtgccttgt gacggctgcc gcttccaggc ccgcatcgcc ttcctcagca
     1921 gcgtcgccta caagggcggc gagctcggcg gagtcgaggg cgcccacctc cggtgtcagg
     1981 cgctcgccca gcaaagcggc ctcgacaact cggccagctt catggcctgg ctcagcgacg
     2041 cccagcacag cccgctccaa gatttcaaaa atggccccga caccgccggc ctcccctacg
     2101 tccgccccga cggcatccgc atcgccgacg actggaacga cctcctgctg aacggcccgg
     2161 gcgacggcat caccgtcacc gagaccggcg agctacagtt gatcaagggc gtgtggaccg
     2221 gcaccgcccc gagcgggggg gtcttcgatc ccgcggcgac ttgcaaggca tggagcagct
     2281 ccaaggcgat cgaccagagc cgcatcggca ccagcggcgt cgacgagcag caggtcgacg
     2341 cgtggatgca gtgggtctct ttcaaacagt ggtccagctt taagagcatt gcctgccaaa
     2401 acacttacca aatttactgt tttgagcagt gattagaggt cgaaccatga aacaccggac
     2461 acgtcttctc gaccgcgttt cgcgtgtgtg cgccgtggtc gccggcagcg tgctgaccag
     2521 cgcttgcttc gtctccgaca tcgaggcgat catcgccgat tacgaggtca ccgaagcacc
     2581 gggcagctcc gacgagggca gctcgagtca ggatgcatcg acggggacga gtacgggcat
     2641 gaccgctgac agcaccggcg gcccgggcac ctcgacctct ggcgtcgacg agaccacgag
     2701 cacaggctcg ggctccggcg acagcagcag cacgggcacg acaggtcccg tgtgtggcga
     2761 cggactcgtg gaaggcgacg agacctgcga cgacggcaac gacatccccg gcgacgggtg
     2821 tcagtcctgc gccgccgact cgatcgtctt catcaccagc gagcgttacc agggcttcaa
     2881 gctcggcggc ctgtacggcg ccgatcaacg ctgtcgtaat ctcgcagcca aggccggtct
     2941 ggcccgcccc ctgaccttca tggcctggct gagcacctcg acgatgtccg tcgccgatcg
     3001 catgctgcac tcgcgcggcc gctatgtgct cgtcaacggc ctcgtcgtcg cccaggactg
     3061 ggatgctctc acctcgggca ccctcgagac cccgatcgcc gtgaccgaat actccgaaac
     3121 ccgggaagac ctcgtctgga ccggcacctt gccgggtggg cagccgtccc tgggctccga
     3181 cttctgcggc gactgggacg acccctcggc cttcaacgaa gacggcggta gcggctacag
     3241 cctggccacc gacgcctggt ggtccttctt cgaccataac ggctgtatct ccgagctccc
     3301 cctctactgc gtcgagcagc gcgaggcgcc atgatcggcc ccctgccgtc cgtcctctcg
     3361 cgcgttgccc tggcctcact gctcgtcacc cccgcttgca acggcccgac gagcgaagcc
     3421 agcagcaccg ggaccaccca ggagacgtcg agcaccgcca gcgacgcgac cccgggcacc
     3481 tcgacgacca ccgcccccgc ctcgacgagc acgcagacag gtggcctcga tacatcaac
//
LOCUS       DASWNJ010000746         4774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_578949, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000746 DASWNJ010000000
VERSION     DASWNJ010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578949"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VGB85_12495"
     CDS             complement(<1..210)
                     /locus_tag="VGB85_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEY0134901.1"
                     /translation="MSATELVIRGRRVVTPEGVRAASVHVSEGRIVAVAEHDAVPAGA
                     KLVDVGDAVVMPGVVDTHVHVNEPGR"
     gene            complement(236..1444)
                     /locus_tag="VGB85_12500"
     CDS             complement(236..1444)
                     /locus_tag="VGB85_12500"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEY0134902.1"
                     /translation="MLDHLYELLHLIARWVHLIAGVMWIGNSLLFNWLDRNLERAKDA
                     KAGVVGTIWMIHSGGFYEVEKKALEPGQMPATLHWFKWQNGVTWLSGISLLILVFYLA
                     ASSTMVDPDISNITSQTAVLISAVSLPLSYLAYDLVWRTRIGKRPGLATFLTIVVVVA
                     TSAVYFACFSGRAAYIHVGVLLGTLMTGNVWLVIMPSQRELVAATIAGREQDPAPGYQ
                     AKSRSIHNNYFTFPLLFIMLSGHFPSTFSHPQSWLILAVLALGSAGIRHLMNVRFTNP
                     LWLFPAVSLFIITFLFTLVMMSDRDILGSGLPARTTPVAFGEVADIVHKRCTNCHSAT
                     PSDAVYRSAPNGVMFDTPDQILRKAEDIKLRAVTLKNMPLANITGITLEERAALGAWV
                     DQGAKGPEAP"
     gene            1627..4158
                     /gene="uvrA"
                     /locus_tag="VGB85_12505"
     CDS             1627..4158
                     /gene="uvrA"
                     /locus_tag="VGB85_12505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY0134903.1"
                     /translation="MDASQGSGDRTGFVQVRGARENNLKGVDLEVPRDALVVFTGVSG
                     SGKSSLAFGTLFAEAQRRYLESVAPYARRLFEQMRAPQVDSIDGLPPAVALQQQRGGA
                     TSRSSVGSVTTLSNLLRMLYSRAGDYPKGQGILYAESFSTNTPEGACPGCHGLGRIYE
                     VTERSLVPDPALSIRERAIAAWPTAWHGQNLRDICVTLGFDIDRPWKELAKKDRDWLL
                     FTDEQPVVPVYAGFTTEEVRRAIKRKAEPSYQGTFTSARRYVLHAFANTQSASIKRRV
                     SQYMLSSECPQCHGKRLRPEPLSVTFAGLDIAEISRLSLAELMEVMRPHAEGRAPGMA
                     RLKHDHPERAEVAQRIAADLLARLGVLLDLGLGYLTLERTTPTLSPGELQRLRLATQV
                     RSNLFGVVYVLDEPSAGLHPADTEALLVALGRLKASGNSLFVVEHDLDVIRRADWIVD
                     VGPAAGEQGGEILYSGPPAGLDRVPASQTRLHLFGHVRPPARTPRTPRGWLRLAGVTR
                     NNLEGLDVAFPLGVFTAVTGVSGSGKSSLVSQALVELVAAQLGHELAEEEEEGEALER
                     TPQAPTGGKITGGMETIKRLMRVDQRPIGRTPRSNLATYTGLFDHVRKLFSATKMARA
                     RHYDAGRFSFNVAKGRCETCEGEGFVSVELLFMPSVYAPCSTCHGARYNAKTLEVLYA
                     GKNIAEVLAMTVHEAHAFFAGEPAVQRSLHVLREVGLGYLRLGQPATELSGGEAQRIK
                     LATELQRMSRGSTLYVLDEPTTGLHPSDVDRLMTQLDSLVAAGNTVIVVEHDMRVVAG
                     SDHVIDVGPGAGTAGGRVVASGSPAEVAKARDSRTAPYLARYLAG"
     gene            4348..>4774
                     /locus_tag="VGB85_12510"
     CDS             4348..>4774
                     /locus_tag="VGB85_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134904.1"
                     /translation="MRNLTLSLITTSILALSACGGEPDESTTLATGTTGGTTGGTETT
                     ASTNPTSVSATEPNDTTSGGTTTGNSSNPTTGPTTGEPGTTTGSSTTIEPGTAGSSDA
                     TTGEGTTGEGTTGEGTTGGGTTGAVDCAGFGPMECMAQDA"
BASE COUNT          749 a         1566 c         1765 g          694 t
ORIGIN      
        1 gcggccgggc tcgttgacgt gcacgtgcgt gtcgacgacg ccgggcatga cgacggcgtc
       61 gccgacgtcg acgagcttcg cgcccgcggg cacggcgtca tgctcggcga cggcgacgat
      121 gcgcccctcg gatacgtgca ccgacgcggc gcgcacgccc tcgggcgtga cgacgcgtcg
      181 cccgcggatc accagctcgg tggcgctcat ggtctcaccc cacaattacg atcgttcatg
      241 gtgcctccgg tcccttcgcg ccctggtcga cccaggcgcc gagcgccgcg cgctcctcga
      301 gcgtgatgcc cgtgatgttg gcgagcggca tattttttaa agtgacggcg cgcagcttga
      361 tgtcttcggc cttgcgcagg atctggtcgg gggtgtcgaa catcaccccg ttcggcgcgg
      421 agcggtagac cgcgtcgctc ggggtcgccg agtggcagtt ggtgcagcgc ttgtgcacga
      481 tgtcggcgac ctcgccgaac gcgacggggg tggtccgcgc gggcagcccg gagccgagga
      541 tgtcgcggtc cgacatcatc acgagggtga acaggaacgt gatgatgaac agcgacaccg
      601 ccgggaacag ccacagcggg ttggtgaagc gcacgttcat caggtggcgg atgccggcgc
      661 tgccgagcgc gagcacggcg aggatcagcc agctctgtgg gtgactgaag gtcgagggga
      721 agtgcccgct gagcatgatg aacagcagcg ggaaggtgaa gtagttgttg tggatcgacc
      781 tcgacttggc ctgatagccg ggcgcggggt cttgctctct gcccgcgatc gtcgcggcga
      841 cgagctcgcg ctgcgagggc atgatgacga gccagacgtt gccggtcatc agggtgccga
      901 gcagcacgcc gacgtggatg tacgcggcgc gcccgctgaa acaggcgaag tagaccgcgg
      961 aggtggcgac gacgacgacg atcgtcaaaa aggtcgccag cccgggccgc ttgccgatcc
     1021 gcgtgcgcca tacgaggtcg taggcgaggt aggacagcgg cagcgagacc gcgctgatca
     1081 gcaccgcggt ctgcgaggtg atgttcgaga tgtccgggtc gaccatcgtc gacgaggcgg
     1141 cgaggtagaa cacgaggatc agcagggaga tgcccgacag ccaagtgacg ccgttttgcc
     1201 acttgaacca gtgcagggtc gccggcatct ggcccggctc gagcgccttc ttctcgacct
     1261 catagaaccc gccggagtgg atcatccaga tcgtgccgac gacgcccgcc ttggcatcct
     1321 tcgcgcgctc gaggttgcgg tcgagccagt tgaacagcag cgagttgccg atccacatga
     1381 cccccgcgat cagatgcacc cagcgggcga tcaggtggag gagctcgtag aggtggtcga
     1441 gcatgcgccg ccggatgata cagacagcgc gacggcgggg catcgtcttc tccgccgcct
     1501 gcaagacgtg tgggcgcgtc cggcgcacgc ggtcgtggcg ggatatttgt cgcggccgac
     1561 gcggccctcc cggcccgcat accggcccgc gcggcccgcc gccgcggctt tgctatgcgg
     1621 ggccgcatgg acgcatcgca gggcagcggc gaccggaccg gcttcgtgca ggtccgcggg
     1681 gcccgcgaga acaacctcaa gggcgtcgac ctcgaggtcc cgcgcgacgc gctggtggta
     1741 ttcacggggg tctcgggatc cggcaagtcg tcgctggcct tcggcacgct gttcgcggag
     1801 gcgcagcggc gctacctcga gtcggtcgcc ccctacgcgc gccgactgtt cgagcagatg
     1861 cgggcgccgc aggtcgacag catcgacggc ctgccgccag cggtcgcctt gcagcagcag
     1921 cgcggcgggg cgacctcgcg gtcctcggtc ggcagcgtga cgacgctgtc gaacctgctg
     1981 cgcatgctct actcgcgggc cggggattac ccgaagggcc agggcatcct gtacgcggag
     2041 tcgttctcga ccaacacgcc ggagggcgcg tgccccggat gccacgggct cggaaggatc
     2101 tacgaggtca cggagcggtc gctcgtgccg gacccggcgc tgtcgatccg cgagcgggcg
     2161 atcgccgcgt ggccgaccgc gtggcacgga cagaatctgc gcgacatctg cgtgaccctc
     2221 ggcttcgaca tcgaccggcc gtggaaggag ctggcaaaaa aggaccgcga ttggctgctg
     2281 ttcaccgacg agcagccggt cgtgccggtg tacgccggct tcacgaccga ggaggtgcgc
     2341 cgcgcgatca agcgcaaagc ggagccgtcg tatcagggca cgtttacctc ggcgcggcgc
     2401 tatgtactgc atgcattcgc aaatacccag agcgcgtcga tcaagcgccg cgtatcacag
     2461 tacatgctca gcagcgagtg cccgcagtgc cacggcaagc gcctgcgacc ggagccgctg
     2521 tcggtcacct tcgccgggct cgacatcgcc gagatctcgc ggctgtcgct cgcggaattg
     2581 atggaggtca tgcgaccgca cgccgagggc cgcgcgccgg gcatggcgcg cctcaagcac
     2641 gaccacccgg agcgcgccga ggtggcgcag cgcatcgccg ccgacctgct cgcgcggctc
     2701 ggggtgctgc tcgacctcgg gctcgggtat ctgacgctcg agcggaccac gccgacgctg
     2761 tcgcccggcg agctgcagcg cctgcgcctg gcgacgcagg tgcgctcgaa cctgttcggc
     2821 gtcgtctacg tgctcgacga gccctcggcc ggcctgcacc cggcggacac cgaggcgttg
     2881 ctggtcgcgc tcgggcggct caaggcgtcc gggaattcat tgttcgtggt cgagcacgac
     2941 ctcgatgtca tccgccgcgc cgactggatc gtcgatgtcg ggcccgcggc gggcgagcag
     3001 ggcggcgaga ttctatatag tgggccgccg gcgggcctcg atcgggtgcc ggcctcgcag
     3061 acgcggctgc acctgttcgg ccacgtccgg ccgccagcgc gcacaccacg gacgccgcgg
     3121 gggtggttac ggctcgccgg ggtgacgcgc aacaacctcg aggggctcga cgtcgcgttc
     3181 ccgctgggcg tgttcacggc ggtgacgggg gtgtcgggct cgggcaagtc gagcctcgtc
     3241 agccaggcac tggtggagct ggtcgccgcg cagctcgggc acgagctcgc cgaggaggag
     3301 gaggagggtg aggcgctcga gcgcacgccg caggcgccga ccggcggcaa gatcaccggc
     3361 ggcatggaga cgatcaagcg gctgatgcgg gtcgaccagc ggccgatcgg gcgcacgccg
     3421 cggtcgaacc tcgcgaccta cacggggctg ttcgaccacg tgcgcaagct gttctcggcg
     3481 accaagatgg cgcgggcccg ccactacgac gccggccgct tctcgttcaa cgtcgccaag
     3541 ggccgctgcg agacctgcga gggcgagggc ttcgtgtcgg tggaattgtt gttcatgccg
     3601 agcgtgtacg cgccgtgctc gacctgccac ggcgcccgct acaacgcgaa gacgctcgag
     3661 gtgctgtatg cgggcaagaa catcgccgag gtgctggcga tgacggtgca cgaggcgcac
     3721 gcgttcttcg ccggggagcc ggcggtgcag cgctcgttgc atgtgctgcg cgaggtcggc
     3781 ctcgggtatc tccggctcgg tcagccggcg acggagctgt cgggcggcga ggcccagcgg
     3841 atcaagctgg cgaccgagct gcaacggatg tcccgcggct cgacgctgta cgtgctcgac
     3901 gagccgacca ccggcctgca cccgtcggac gtcgatcgac tgatgactca gctggattcg
     3961 ctggtcgccg cgggcaacac cgtgatcgtg gtcgagcacg acatgcgcgt cgtcgccggc
     4021 agtgatcacg tgatcgacgt cgggcccggc gcgggcacgg caggcggccg cgtggtcgcc
     4081 agcggatcgc cggccgaggt ggcaaaagcg agggacagcc gcacggcgcc gtacctcgcg
     4141 cgctacctgg cgggatgagg cgcccgatcg gtggattcgc cgggcgtgtc tgcatatgtg
     4201 atcgggtgag attgtcggtt cgatacgggg acagggctgc ccggagcaat tccagcgtcg
     4261 caggaaatcc tggtcgtgcg tcgtcgccgg gcgtgggccg gcctctgcgg actgtcgggg
     4321 gcgcgacgcg gccgatagcg tgatggcatg cgaaacttga ccttgtctct gatcacgacc
     4381 tcgatcctcg cgctgtcggc ctgtggcggc gaaccggacg agagcaccac cctcgcgacc
     4441 ggtacgaccg gcggcacgac cggcggcacc gagaccacgg cgtcgaccaa cccgacctcc
     4501 gtctcggcga ccgagccgaa cgacacgacg agcggcggca cgaccacggg taattccagc
     4561 aacccgacga cgggcccgac gaccggcgag cccggcacga cgaccgggtc gagcacgacg
     4621 atcgagccgg gcaccgcggg cagctccgac gccacgacgg gcgagggcac gacgggcgag
     4681 ggcacgacgg gcgagggcac gacgggcggt ggcacgaccg gtgcggtcga ctgcgcgggg
     4741 ttcgggccga tggagtgcat ggcgcaggac gcct
//
LOCUS       DASWNJ010000747         2308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_506992, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000747 DASWNJ010000000
VERSION     DASWNJ010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506992"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="VGB85_12515"
     CDS             complement(<1..848)
                     /locus_tag="VGB85_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134905.1"
                     /translation="MRRRPRSPLCLLACLSCLPLAGLACTPKATSTPPIAHTPALPTA
                     APSADSGKDDKPKWDVTDPPGDETDVAIDVREGTWIALDISPDGRELVFDLLGDLYLV
                     PLAGGEARALTSGMAWDMQPRFSPDGQWIAFTSDRGGGDNVWVLPRSGDGKAAEPRQI
                     TKEDFRLVNSPVWSPDGNYIAVRKHFTKHRSLGSGEIWLYHSSGGKGVQMTEKPNDQK
                     DVGEPAFSPDGKYVYFSQDITPGPTFEYNKDPYGEIYAITRLDRGDGRIEKLVGGPGG
                     AARPTPS"
     gene            947..2299
                     /locus_tag="VGB85_12520"
     CDS             947..2299
                     /locus_tag="VGB85_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134906.1"
                     /translation="MAVQERDDDGLMLPPLDDRRWLQDQLKALVAARGVAPLATALLV
                     EPSARYFPDPWGGGEPSVRRLLSRVCAYAGMAGLRVAVRIYDAEPSRRGEVVGKPPVR
                     NGQELAVWLAKVSPAPGGRPDHGEIEVGVESSALVDPASLVAACARVISHAYRVVHGL
                     ATTDAYDPRIDVTAVYLGFGMLTADAALRFVTRTSTGVMQTRRTPLRLGSLPPQGVCF
                     LLAAQIHVRGEGRAERQRLAKNLQANQAAFFRHAFAAIERMDPPIAVQLGVPARDHWP
                     TPPSLAELTAPLPDAGSSEPETRLDEDRGVAGMNRGKPVFMVDRSMAPRLGRVLFMGA
                     LMLGGVLSRMSPGSGGGMSNIAVIAAGLGLAGLGIGRLFRDRRCSEPKCGAPLRPALL
                     VCPRCSGNIVGTIGHPKERLAAEEAHLKALSATPAESLTDGTAPVAVPAAPDTNTPPA
                     "
BASE COUNT          296 a          824 c          858 g          330 t
ORIGIN      
        1 gagggcgtcg ggcgggccgc gccgccgggt ccaccgacga gcttctcgat ccgtccgtcg
       61 ccgcgatcga ggcgcgtgat cgcgtagatc tcgccgtagg ggtccttgtt gtactcgaag
      121 gtcggcccgg gcgtgatgtc ctggctgaag tacacgtact tgccgtcggg cgagaaggcc
      181 ggctcgccga catccttctg gtcgttcggc ttctcggtca tctgcacgcc cttgccgccc
      241 gacgagtggt acagccagat ctcgccgctg ccgaggctgc ggtgcttggt gaagtgcttg
      301 cgcaccgcga tgtagttgcc gtcgggcgac cagaccgggc tgttgaccag gcgaaagtcc
      361 tccttcgtga tctggcgcgg ctcggccgct ttcccatcgc cagagcgcgg cagcacccac
      421 acgttgtcgc cgccgccgcg gtcgctggtg aacgcgatcc actggccgtc ggggctaaag
      481 cgcggctgca tgtcccaggc catgcccgag gtcagcgcgc gcgcctcacc gccggcgagc
      541 ggcacgaggt agaggtcgcc gagcaggtcg aacaccagct cgcggccgtc ggggctgatg
      601 tcgagggcga tccaggtgcc ctcgcggacg tcgatcgcga catcggtctc gtcgcccggc
      661 ggatcggtga cgtcccactt cggcttgtca tccttgccgc tgtccgcgga cggcgccgcg
      721 gtcggtagtg cgggcgtgtg ggcgatcggc ggggtgctgg tcgccttcgg cgtgcaggcg
      781 aggccggcga gcgggaggca agacagacag gcgaggaggc acaggggcga gcgaggccgg
      841 cggcgcatgg gcgcgcatag tacccgcgag ccagggtttc atgcagctcc gggcgacagc
      901 tcacgcgccc ggcagacggc gggcgagtcg tggtagacag cgcgtcatgg cggtccagga
      961 acgcgacgac gacgggctca tgctgccgcc gctcgacgac cggcgctggc tgcaggacca
     1021 gctcaaggcc ctcgtcgcgg cccgcggcgt ggcgccgctg gcgaccgcgc tgctggtcga
     1081 gccgagcgcc cgctacttcc ccgacccgtg gggcggcggc gagccgagcg tgcgccgctt
     1141 gctgtcgagg gtctgcgcat atgcagggat ggccgggctg cgcgtcgcgg tgcgcatcta
     1201 cgacgccgag ccgagccgcc gcggcgaggt cgtcggcaag ccgccggtgc gcaacggtca
     1261 ggagctggcg gtgtggctgg ccaaggtgtc gccggccccg ggcggtcggc ccgaccacgg
     1321 cgagatcgag gtcggcgtcg agtcgagcgc gctcgtcgat ccggcgagcc tcgtcgccgc
     1381 gtgcgcgcgc gtgatcagcc acgcgtatcg tgtcgtgcac gggctcgcca ccaccgatgc
     1441 atacgacccg cggatcgacg tcaccgcggt gtacctcggc ttcggcatgc tgaccgccga
     1501 cgccgcgctg cgcttcgtca cccgcacgag caccggcgtg atgcagaccc ggcgcacgcc
     1561 gctgcgcctc ggcagcctgc cgccgcaggg cgtgtgcttc ctgctcgccg cgcagatcca
     1621 cgtgcgcggc gagggccggg ccgagcgcca gcgcctcgcc aagaacctcc aggccaacca
     1681 ggccgcgttc tttcgccacg ccttcgcggc gatcgagcgc atggacccgc cgatcgcggt
     1741 gcagctcggc gtgcccgcga gggaccactg gccgacccca ccctcgctcg ccgagctgac
     1801 cgcgccgttg cccgacgcgg gttccagcga gcccgagacg cggctcgacg aggaccgcgg
     1861 cgtcgccggc atgaaccgcg gcaagccggt gttcatggtc gaccgcagca tggcgccgcg
     1921 cctcggccgc gtgctgttca tgggcgcgct gatgctcggc ggcgtgctgt cgcggatgtc
     1981 gccgggcagc ggcggtggca tgagcaacat cgccgtgatc gcggccggcc tcggcctcgc
     2041 gggcctcggc atcgggcgat tgtttcgcga ccgccgctgc tcggagccca agtgcggcgc
     2101 cccgctgcgg ccggcgctgc tggtgtgccc gcgctgcagc ggcaacatcg tcggcacgat
     2161 cggccacccg aaggagcgcc tcgccgccga ggaggcgcac ctcaaggcgt tgtcggcgac
     2221 gccggccgag tcgttgaccg acgggaccgc gcccgtggcc gtaccggcgg ccccggacac
     2281 gaacacgccg ccggcctgat atgacacc
//
LOCUS       DASWNJ010000748         4790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_649854, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000748 DASWNJ010000000
VERSION     DASWNJ010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649854"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..1923
                     /locus_tag="VGB85_12525"
     CDS             262..1923
                     /locus_tag="VGB85_12525"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEY0134907.1"
                     /translation="MCRRDGWISLNGAWDFALDADARWSQPGEVAWAGPIEVPFSPET
                     PRSGVAATGYFQACWYRRSVRPPPLGPDERLLLHFGAVDHLATVWVDGAQVATHEGGY
                     TPFTVDLTPLLRGQPLEVVVRAADDPHDLAKPRGKQDWLREPHSIWYPRTSGIWQTVW
                     LERVPPVSIAKLCWASNLMRWEIDASVILQGRFRPGLSLRLRLSVGDLLLADDLCQLT
                     HPELYRRIALSDPGIDDARNALLWSPETPTLIDARIELRDADGRVIDAVDSYTAIRTI
                     GIEGDRIILNGRPIKLRMVLDQGYWPDTGLSAPDDEALRRDVELAKAMGFNGVRKHQK
                     VEDPRYMYWADRLGLMVWGEMPSAYRFTQVSVERLTREWMAVIRRDESHPCIIAWVPF
                     NESWGVPNLPDNPSERHYVQALYHLTKTLDPTLLDARYQSPEIQQNLKTQRFGGRILV
                     IQGHRCDHPLILSEFGGVKLSDEEGTWGYARSATPRQLERRLRALFEVVRQQELFAGY
                     CYTQFADTYQEANGLLHADRTPKFPLERIAALNGVGPREPSHEGS"
     gene            1926..3005
                     /gene="galT"
                     /locus_tag="VGB85_12530"
     CDS             1926..3005
                     /gene="galT"
                     /locus_tag="VGB85_12530"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEY0134908.1"
                     /translation="MHRLDLRKPDGRALHLYAREPLPPDIVATGPQGGKPGSHLRWHP
                     LRGEWVVYATHRQTRTFLPPPEFNPLRPGTDPAFPTEVPAGPWEVAVFENLFPALSPH
                     AGAAPVCDVETRPGVGACEVVVFTQDPNASLGGLPLDRIELIMQVWGDRYTEIGGRDE
                     IAYVFPFENRGVEVGVTLHHPHGQIYAYPFVPPIPARELEQQRAYYQKHGRGLLADMI
                     ARERADGRRVLYAGEHAIAYVPVCARYAYEVWVSPIEPVPSFAALDDATRADLARALK
                     TVLCKYDALWQRPFPYLMACKQAPTDGADHPEAHLQIELYPPYRMPGRLKYLAGTEIG
                     AGVFTADTLPEDKAAELRAVEVHID"
     gene            2998..4107
                     /gene="galK"
                     /locus_tag="VGB85_12535"
     CDS             2998..4107
                     /gene="galK"
                     /locus_tag="VGB85_12535"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEY0134909.1"
                     /translation="MTESPEFRALFGRDPEVFASAPGRVNLIGEHTDYNDGLVLPAAI
                     PRRTHLELARRDDGRVQVSSADVRGGTLGYRLGEETRQGRWIDYVQGITHVLADRGLS
                     GFDLRIHSQVPLGSGLSSSAALEVGLLRGLRELFSLAIEDVALALLAHQAETQFVGAP
                     VGIMDPFACHFADETHALLLDTRSRAIEQVPLPTGLGLVVIDSGVHHQLAAQGGYRTR
                     RGECEQAAARLGLTSLRALEDLDRVATLPEPLARRVRHVFSENDRVLATVRALRAGDL
                     EAIGALFCASHRSMRDDYEVSVAEVDALVEIADAEPAVYGARLTGGGFGGSVVIAVRP
                     DDIAGVATRIARTHAQATGRPSRVLVPALDLAPGP"
     gene            4138..>4790
                     /gene="galE"
                     /locus_tag="VGB85_12540"
     CDS             4138..>4790
                     /gene="galE"
                     /locus_tag="VGB85_12540"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEY0134910.1"
                     /translation="MRVLVTGGAGYIGSVVVEELVRDGHEVVVLDNLSKGHPEAVAPG
                     VPLVRADLRDRATVEATLRERGIEAVVHMAADSLVGESVQKPGKYYDNNVVAGLALLD
                     AMVACDVKRLVFSSTAAVYGEPAKQPIEEHDPTVPTNPYGETKLAFERALPWYEGAHG
                     LRSASLRYFNAAGASDRCGEMHDPETHLIPIVLQAAAGLRDIVTIYGEDYPTPDGTC"
BASE COUNT          678 a         1805 c         1623 g          684 t
ORIGIN      
        1 gatcctccgg cggtcgccca cgcgaaccaa tcgactccgc gcaggcggat cgccgcgagc
       61 cccgcagcgg tgagggctgt acggatgatc cccagtttct cggcaagctc ggtcatgttg
      121 tgtcgcgtct gacttggcag ccttgcaagg tcggtgcaag gaggaacgat gccgagtccc
      181 gagccgcccc cccaggagcc tgcaggcccc accggttccc gcgccgacga cctcgccctc
      241 ggtcgcggcc atccgcgccc gatgtgccgc cgtgacggct ggatcagcct caatggcgcg
      301 tgggacttcg cgctcgacgc cgacgcccgc tggtcgcagc ccggcgaggt cgcgtgggcc
      361 ggcccgatcg aggtcccgtt ctcgcccgag accccgcgca gcggcgtcgc tgcgaccggc
      421 tactttcagg cctgctggta tcgccgcagc gtccgcccgc cgccgctcgg ccccgacgag
      481 cggctcctgc tgcacttcgg cgcggtcgac cacctcgcca cggtgtgggt cgacggtgcg
      541 caggtggcga cccacgaggg cggctacacc cccttcacgg tcgacctgac cccgctgttg
      601 cgtggccagc cgctcgaggt cgtcgtgcgc gcggccgacg atccgcacga cctggccaag
      661 ccgcgcggca aacaggactg gctgcgcgag ccgcactcga tctggtaccc gcgcacctcg
      721 gggatctggc agaccgtgtg gctcgagcgc gtgccgccgg tgtcgatcgc caagctgtgc
      781 tgggcgtcga acctgatgcg ctgggagatc gacgccagcg tgatactgca gggccgcttt
      841 cggcccggcc tgtcgctgcg gctgcgcctg agcgtcggcg atctgctgct cgccgacgac
      901 ctgtgccagc tgacccaccc cgagctgtac cgccggatcg cgctctccga tcccggcatc
      961 gacgacgccc gcaacgcgct gctgtggagc ccggagacgc cgacgctgat cgacgcgcgc
     1021 atcgagctcc gcgacgcgga cggccgggtg atcgacgcgg tcgacagcta cacggcgatc
     1081 cgcacgatcg ggatcgaggg cgatcgcatc atcctcaacg gccggccgat caagctgcgc
     1141 atggtgctcg accagggcta ctggcccgac accggcctga gcgcgcccga cgacgaggcc
     1201 ctgcgccgcg acgtcgagct cgccaaggcg atgggcttca acggcgtgcg caagcaccag
     1261 aaggtcgagg acccgcgcta catgtactgg gccgaccgcc tcggcctgat ggtctggggc
     1321 gagatgccga gcgcctaccg cttcacgcag gtctcggtcg agcggctgac ccgcgagtgg
     1381 atggcggtga tccggcgcga cgagagccac ccgtgcatca tcgcctgggt gcccttcaac
     1441 gagtcctggg gcgtgcccaa cctgccggac aacccgtccg agcggcacta cgtgcaggcg
     1501 ctctatcacc tcacgaagac gctcgacccg acgctgctcg acgctcgcta tcaatcgccg
     1561 gagatccagc agaacctcaa gacccagcgc ttcggcgggc ggatcctcgt catccagggc
     1621 caccgctgcg atcacccgct gatcctgagc gagttcggcg gggtcaagct gtcggacgaa
     1681 gagggcacct ggggctacgc gcgctcggcc acgccgcgcc agctcgagcg gcgcctgcgg
     1741 gccctgttcg aggtcgtgcg ccagcaggag ctgttcgccg gctactgcta cacgcagttc
     1801 gccgacacct atcaggaggc caacggcctg ctgcacgccg accgcacccc gaagttcccg
     1861 ctcgagcgga tcgccgcgct caacggcgtc ggtccccgcg agcccagcca cgagggctca
     1921 tgagcatgca ccgactcgat ctgcgcaagc ccgacggccg cgcgctgcac ctgtacgccc
     1981 gcgagccgct gccgcccgac atcgtcgcca ccgggccgca gggcggcaag ccgggctcgc
     2041 acctgcgctg gcacccgctg cgcggcgagt gggtcgtcta cgcgacgcac cgccagaccc
     2101 gcaccttctt gccgccgccg gagttcaacc cgttgcgccc cggcaccgac cccgcgttcc
     2161 cgaccgaggt ccccgcgggg ccgtgggagg tcgcggtgtt cgagaacctg ttccctgcgc
     2221 tcagccctca cgccggcgcg gcgccggtct gcgacgtcga gacgcgcccg ggcgttggcg
     2281 cctgcgaggt cgtcgtgttc acgcaggacc cgaacgccag cctcggcggc ctgccgctcg
     2341 atcgcatcga gctgatcatg caggtctggg gcgaccgcta caccgagatc ggcgggcgcg
     2401 acgagatcgc ctacgtgttc cccttcgaga accgcggcgt cgaggtcggc gtcacgctgc
     2461 atcaccccca cggccagatc tacgcctatc ccttcgtgcc gccgatcccc gcgcgcgagc
     2521 tcgagcagca gcgcgcgtac tatcaaaagc acggccgcgg cctgctcgcc gacatgatcg
     2581 cgcgcgagcg cgccgacggc cgccgggtgc tctacgccgg cgagcacgcg atcgcctacg
     2641 tgccggtgtg tgcccgctac gcgtacgagg tctgggtctc gccgatcgag cccgtgccct
     2701 cgttcgcggc gctcgacgac gcgacgcggg ccgacctcgc gcgcgcgctc aagacggtcc
     2761 tgtgcaagta cgacgcgctg tggcagcgac ccttcccgta cctgatggcc tgcaagcagg
     2821 ccccgaccga cggcgccgac caccccgagg cgcatttaca aatcgagctg tatccgccgt
     2881 accgcatgcc cggccgcctc aagtacctcg cgggcaccga gatcggcgcg ggcgtgttca
     2941 cggccgacac gctgcccgag gacaaggccg cggagctgcg cgcggtcgag gtgcacattg
     3001 actgagtcac ccgagtttcg cgcgctgttc ggccgcgacc ccgaggtctt tgcgagcgcg
     3061 cccggccgcg tcaacctgat cggcgagcac accgactaca acgacggcct cgtgctgccc
     3121 gccgcgatcc cgcgacgcac gcacctcgag ctggcgcggc gtgacgacgg ccgcgtgcag
     3181 gtgtcgagcg ccgatgtccg cggcggcacg ctcggctacc gcctcggcga ggagacccgc
     3241 cagggccgct ggatcgacta cgtgcagggc atcacccacg tgctcgcgga ccgcgggctg
     3301 tcgggcttcg acctgcggat ccactcgcag gtgccgctcg gcagtggttt gtcgtcgagc
     3361 gcggcgctcg aggtcggcct gctccgcggc ctgcgcgagc tgttctcgct ggcgatcgag
     3421 gatgtcgcgc tcgcgctgct cgcccaccag gccgagacgc aattcgtcgg cgccccggtc
     3481 ggcatcatgg accccttcgc gtgtcatttc gccgacgaga cccacgcgct cctgctcgac
     3541 acccgcagcc gcgcgatcga gcaggtgccg ttgccgacgg gcctcgggct cgtcgtgatc
     3601 gactccggcg tgcaccacca gctcgccgcg cagggcggct accggacccg tcgcggcgag
     3661 tgcgagcagg cggccgcgcg gctcggcctg acgtccctgc gcgcgctgga ggacctcgac
     3721 cgcgtcgcca cgctgccgga gccgctcgcc cggcgcgtcc gccacgtatt ctcggagaac
     3781 gaccgcgtgc tcgccacggt gcgcgccttg cgggccggcg acctcgaggc gatcggggcg
     3841 ctgttttgtg cctcgcatcg atcgatgcgc gacgactatg aagtgtccgt ggccgaggtc
     3901 gacgccctcg tagagatcgc ggacgccgag cccgctgtgt acggcgcgcg cctcacaggc
     3961 ggcggcttcg ggggatccgt ggtgatcgcc gtccggccgg acgacatcgc cggggtcgcg
     4021 acccgcatcg cccgcaccca cgcgcaggcg accggcaggc cctcgcgcgt gctggtcccc
     4081 gcgctcgatc ttgcgccggg cccgtgatcg gctcataagc cgtgccacgg agtccccatg
     4141 cgagttctcg tgacaggcgg cgcaggttat atcggcagcg tggtggtcga ggagctggtc
     4201 cgcgacggcc acgaggtcgt cgtgctcgac aacctctcga agggccaccc cgaggccgtc
     4261 gcgcccggcg tgccgctggt gcgcgccgac ctgcgcgacc gcgccaccgt cgaggcgact
     4321 ctgcgcgagc gcggcatcga ggccgtcgtg cacatggccg ccgactcgct cgtcggcgag
     4381 tcggtgcaaa agcccggcaa gtactacgac aacaacgtcg tcgccggcct cgcgttgctc
     4441 gacgcgatgg tcgcctgcga cgtcaagcgc ctggtgttct cgtcgaccgc ggcggtctac
     4501 ggcgagccgg cgaagcagcc gatcgaggag cacgatccca ccgtcccgac caacccctac
     4561 ggcgagacca agctggcctt cgagcgcgcc ctgccgtggt acgagggcgc ccatggcctg
     4621 cgcagcgcca gcctgcgcta cttcaacgcc gctggcgcga gcgatcgctg cggcgagatg
     4681 cacgaccccg agacgcacct gatcccgatc gtgctgcagg ccgcggccgg cctgcgcgac
     4741 atcgtgacga tctacggcga ggattacccg acccccgacg gcacctgcat
//
LOCUS       DASWNJ010000749         4228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_326339, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000749 DASWNJ010000000
VERSION     DASWNJ010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326339"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VGB85_12545"
     CDS             complement(<1..626)
                     /locus_tag="VGB85_12545"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0134911.1"
                     /translation="MQQERSIDPVGKLSLTSLGLRLHGLGPIVALILLCIVGALLNSD
                     FASIDNAMNVLTRTAFIGIIAVGMCFVIISGGIDLSVGSMAALIAGLVILLMNALGEW
                     GAPSVLVVAIGIGFAVVLGALFGLTHGLLITRGRIEPFIVTLGTLGIYRAYLTYSSDG
                     GAITLENALSDTYSPVYYTSLLGVPIPVWVFLLVAVAGGLILNRTAYGR"
     gene            complement(604..2094)
                     /locus_tag="VGB85_12550"
     CDS             complement(604..2094)
                     /locus_tag="VGB85_12550"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEY0134912.1"
                     /translation="MSIAVRFENVVKEFGPVRVLHGVSFDLTPGRVYGLLGENGAGKS
                     TLMKILGGYERLTGGELLVSGEARNFESSREAEAAGIVLIHQEFNLAEDLTIGQNIFL
                     GHERKRGWWLDDAAMRAEAARVLAQVGLNRSPDTPVRQLIVAEKQLVEIAKALARDAR
                     LLIMDEPTATLTPGETEALFRLMGKLKADGVTIIYISHKLDEVERTTDEILVMRDGRF
                     VTREATRDVTRHQMANLMVGRELSDLYPPKDPAPTGGVPRLKVQGLAVPGWAADVSFE
                     VQPGEIFGFAGLVGAGRTELVEGLLGLRPHSVERVELDGREIRIGSPREGADQGLTYL
                     SEDRKGKGLHVSFGLAENLTLMALERYAKPWLQPQAERRALRTAVQDFGIRTGSLDVR
                     ASSLSGGNQQKLALAKVLHPHPKVIVLDEPTRGVDVGAKRDIYYLVQRLAREGHAVIV
                     ISSELMELVGLCHRVAVMRAGRLQATVSAQHLTEEELIAHATGTKH"
     gene            complement(2219..3385)
                     /locus_tag="VGB85_12555"
     CDS             complement(2219..3385)
                     /locus_tag="VGB85_12555"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0134913.1"
                     /translation="MTAESSVEAGHRIRLGMVGGGQGAFIGAVHRFAARLDGEFELVA
                     GALSSDPDRALASGRALGIPVDRTYGSFVEMAGREAKRPDGIEAVAIVTPNHMHFAPA
                     KLFLEAGVHVICDKPLTATLDEAEELARIARRSGKLFVLTHNYTGYPMVRQARAMIAS
                     GEVGEVRVVQAEYAQDWLTERAEDGGSKQAEWRTDPRRSGAGGCIGDIGTHAYNLVSF
                     VTGLELESLCADLSALVPARALDDNAHVLLRFGGGARGMLWASQVAVGNENRLVLRVY
                     GTKGGLEWSQEQPDQLWYTPYGRPRQMITRNGSGADDASRRVSRIPAGHPEGYLEAFA
                     NIYAEAARAVRAARNGTARDPDVVFPTVEDGVKGVAFVDACVRSSAGGASWISV"
     gene            3586..3924
                     /locus_tag="VGB85_12560"
     CDS             3586..3924
                     /locus_tag="VGB85_12560"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEY0134914.1"
                     /translation="MDASRGLRSLTEAIDTATAGGRLVFHVFAALAEFERSVIRERTM
                     AGLEAARARGRKGGRPRSLGERDLAAKAMLRDSGITVEEVARRLGVGPATLYRHLPSG
                     RGSLAEGGAS"
     gene            complement(4048..>4228)
                     /locus_tag="VGB85_12565"
     CDS             complement(4048..>4228)
                     /locus_tag="VGB85_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134915.1"
                     /translation="VGTGPEGAATPARFVNHLSPGQSAEVSVAGAAGTRLDVLELAYD
                     GGLLTVRSVTAQPEG"
BASE COUNT          655 a         1517 c         1400 g          656 t
ORIGIN      
        1 cgtccgtaag cggttcggtt aaggatcagc cctcccgcga ccgccactag taagaatacc
       61 cacaccggaa tggggacacc aagcaggctg gtataataca ccgggctgta ggtgtccgac
      121 agtgcattct ccagagtgat cgcgccacca tccgaagaat acgtaaggta ggcacgatag
      181 atgccaagcg tgcccagcgt gacgatgaac ggctcgatac gcccgcgcgt gatgagcagg
      241 ccgtgcgtca gcccgaacag cgcgcccagc acgaccgcga agccgatgcc gatcgcgacc
      301 accagcaccg acggcgctcc ccactcgccc agcgcgttca tcagcagtat gaccaggccg
      361 gcgatcagcg ccgccatcga gccgaccgac aggtcgatgc cgcccgagat gatcacgaag
      421 cacatgccca ccgcgatgat gccgatgaag gcggtgcggg tgagcacgtt catcgcgttg
      481 tcgatggacg cgaagtcgct gttgagcaac gcgccgacaa tgcacagcag gatcaaggcg
      541 acgatcgggc cgaggccatg cagccgtaga cctaatgaag ttaaggaaag cttgcctacc
      601 ggatcaatgc ttcgttcctg ttgcatgggc gatcagctcc tcttcggtca aatgctgcgc
      661 gctgacggtg gcctgcaagc ggccggcgcg catcaccgcg acgcggtggc atagaccgac
      721 cagctccatc agttcggacg agatcacgat cacggcgtgc ccttcgcggg ccagccgttg
      781 caccaagtag tagatgtcgc gcttggcacc tacgtccact ccacgcgtag gctcgtcgag
      841 cactatcacc ttcggatgcg gatgcagtac tttcgcgagg gccagcttct gctggttgcc
      901 tcccgacagc gaggaggcgc ggacatcgag cgagccggtg cggatcccga aatcctgcac
      961 cgccgtccgc agcgcgcgtc gctcggcctg aggctgcagc cacggcttcg catagcgctc
     1021 cagcgccatc agggtcaggt tctcggccaa gccgaagctg acgtgcaggc ccttgccctt
     1081 gcggtcctcg ctcagatagg tgaggccctg gtcggcccct tcccgcgggc tgccgatgcg
     1141 gatctcgcgg ccgtcgagtt cgactcgttc gacgctgtgc gggcgcaggc cgagaaggcc
     1201 ctcgacgagc tccgtccgcc cggcaccgac caagccggcg aagccgaaga tctcaccggg
     1261 ctggacctcg aagctcacat cggccgccca gcccggcacc gccaatcctt gcaccttcag
     1321 ccgcggaacc ccgcccgtcg gcgccgggtc cttcggcgga tacaggtccg acagctcgcg
     1381 cccgaccatc aggttggcca tctgatgacg cgtgacatcg cgcgtggctt cgcgcgtgac
     1441 gaagcggccg tcgcgcatca cgagtatctc gtcggtggtg cgttccacct cgtcgagctt
     1501 gtgcgagatg tagatgatgg tcacgccgtc cgccttgagc ttgcccatca gcctgaacag
     1561 ggcctcggtt tcgccgggcg tcagggtggc ggtgggctcg tccatgatca gcaaccgcgc
     1621 gtcgcgcgcg agcgccttcg cgatctcgac gagctgcttc tcggccacga tcagctgccg
     1681 caccggcgtg tcggggctcc tgttcaaccc cacctgcgcc agcacgcgcg ccgcttcggc
     1741 gcgcatcgcc gcgtcgtcga gccaccaacc gcgcttcctc tcgtggccga ggaagatgtt
     1801 ctggccgatg gtcaggtcct cggcgaggtt gaactcctgg tggatcagca cgatgcccgc
     1861 ggcctcggcc tcgcgcgagc tctcgaagtt ccgtgcctcg ccactcacga gcagttcgcc
     1921 gccggtgaga cgctcgtagc ccccgaggat cttcatcaac gtggacttgc cggcgccgtt
     1981 ctcgccgagc agcccgtaca cgcggccagg cgtcaaatcg aagctcacgc cgtgcagcac
     2041 ccgcactgga ccgaactctt tgacgacatt ctcgaagcgg accgcgatgc tcatcgaacg
     2101 gctccccgta gcttatctct tgcactgcca gcacgcccgc atcgatcagc cccgctcgct
     2161 tcccgagcga cgtatacgag ccgacatccc cctagacggg ctcggtcggt ggattcggtc
     2221 aaacgctgat ccaactcgcg ccgccggcgc tcgagcgcac gcaggcgtcg acgaaggcga
     2281 cccctttgac accgtcctcg accgtgggga acacgacgtc gggatcgcgc gccgtcccgt
     2341 tccgcgcggc gcggacagca cgcgcggcct cggcgtagat gttggcgaac gcctcgaggt
     2401 agccctcggg atgcccggcc gggatgcgcg acacgcgccg cgacgcgtcg tccgcgcccg
     2461 agccgttgcg cgtgatcatc tgcctgggtc ggccgtaggg ggtgtaccag agctggtcgg
     2521 gttgctcctg ggaccattcg agcccgcctt tggtgccgta gaccctgagc accaggcggt
     2581 tctcgttgcc gacggccacc tggctcgccc agagcatgcc ccgcgcgccg cccccgaagc
     2641 ggaggaggac gtgcgcgttg tcgtcgagcg cgcgggccgg caccaaggcg ctcaggtcgg
     2701 cgcacaggct ctccagctcg agcccggtca cgaagctcac gagattgtag gcgtgggtgc
     2761 cgatgtcgcc gatgcagccg ccggcgccgg agcgcctcgg atcggtacgc cactcggcct
     2821 gcttggatcc gccgtcctcc gccctctcgg tcagccagtc ctgcgcgtac tccgcctgca
     2881 cgacccggac ctcgccgacc tcgccgctcg cgatcatggc gcgggcctgc cggaccatcg
     2941 ggtagccggt gtagttgtgg gtgagcacga agagcttgcc gctgcgtcgc gcgatccggg
     3001 cgagctcctc ggcctcgtcc agcgtcgcgg tcagcggctt gtcgcagatg acgtgcacgc
     3061 cggcctcgag gaacagcttg gcgggcgcga aatgcatgtg gttcggggtg acgatggcga
     3121 ccgcctcgat gccgtccggt cgcttcgcct cccgccccgc catctcgacg aacgagccgt
     3181 acgtgcggtc cacggggatg ccgagcgcgc gccccgaggc caaggcgcga tccgggtccg
     3241 acgagagcgc gcccgcgacc agctcgaact cgccgtcgag acgggcggcg aaccggtgca
     3301 cggcgccgat gaaggcgcct tgcccgccgc ccaccatgcc gagcctgatg cgatgccccg
     3361 cctcgaccga gctttcggcc gtcatcgcac gccttccccc tgttttaccc ggctcagcga
     3421 gcccgctaag agggcttgcc agatcggctc aattggcact cgcagagctg ctcttccgcc
     3481 gacctttagt caatcgcaag tgcacgctgc cggagcggct cggccgcgac gtggcggccg
     3541 gtggtccggc agagaccggc gaagttgttg acccgaactt tcggaatgga cgcctcccgc
     3601 ggcctgcgct cgctcaccga ggcgatcgac accgccaccg ccggcggccg gctggtgttc
     3661 cacgtcttcg ctgcgctcgc cgagttcgag cgctcggtca tccgcgagcg gacgatggcg
     3721 gggctcgagg ccgcacgcgc gcgggggcgc aagggcggcc ggccgcgctc cctcggcgag
     3781 cgcgacctgg cggccaaagc gatgctccgg gactcgggca tcaccgtcga ggaagtggcc
     3841 cggcgccttg gcgtggggcc ggccacgctc taccgccacc tgccgagcgg gcggggctcg
     3901 ctcgcggagg gcggcgcctc gtagcccggg gccgcctcga gcgccctccc cgcccgtccc
     3961 tcgtacggca agccggggat cgtcggccgg aaccgtggca gggacgatgc gcgccggcgg
     4021 acgacgcgtg tcgtccccgc gaccggatca accctccggc tgggccgtga cggaccggac
     4081 cgttagcagg ccaccgtcgt aggcgagctc gagcacgtcc agcctggtgc cggccgcgcc
     4141 cgcgaccgag acctcggcgc tctggcccgg cgagaggtgg ttcacgaacc gggccggggt
     4201 ggccgcgccc tcggggccgg tcccgacg
//
LOCUS       DASWNJ010000750         1905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_507015, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000750 DASWNJ010000000
VERSION     DASWNJ010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507015"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VGB85_12570"
     CDS             <1..233
                     /locus_tag="VGB85_12570"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEY0134916.1"
                     /translation="TERLWGLLVPLLPRYEAEGKSYLTIALGCTGGRHRSVYLAELLA
                     GRLRDAGRDVAVRHRELAEPPPPPPRPAGDAR"
     gene            230..637
                     /locus_tag="VGB85_12575"
     CDS             230..637
                     /locus_tag="VGB85_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEY0134917.1"
                     /translation="MIGLVLVTHGRLAAEFVAALEHVVGPQERVLAVCIGADDDIEAR
                     RAEIVRAIRDVDDGAGVVVLTDMFGGTPSNLAISTMDRKGVEVIAGVNLPMLIKLASV
                     RAAEPLAQAVVAAQEAGRKYINVASRLLAPDKG"
     gene            644..922
                     /locus_tag="VGB85_12580"
     CDS             644..922
                     /locus_tag="VGB85_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEY0134918.1"
                     /translation="MSTGPVTREVTIVNQRGLHARAAAKFVRAAGEFEAAVAVTRDDT
                     TVAGTSILGLMLLGAGIGSTLTLKAEGTDAEAAIAAIADLVERGFDEG"
     gene            1149..>1905
                     /locus_tag="VGB85_12585"
     CDS             1149..>1905
                     /locus_tag="VGB85_12585"
                     /inference="COORDINATES: protein motif:HMM:NF017071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY0134919.1"
                     /translation="MTSQHNDYAVADIGLADWGRREIAIAETEMPGLMAVRAEYGPSQ
                     PLKGARVAGSLHMTIQTAVLIETLVHLGADVRWASCNIFSTQDHAAAAIAKAGVPVFA
                     YKGETLDEYWDYAHRIFDWHDGGVPNMILDDGGDATLLAHLGRKAEQDASVLDDPHNE
                     EEEALYKSMKKRLKSHPGWYTSLGDKIRGVTEETTTGVHRLYDMERKGELLFAAINVN
                     DSVTKSKFDNLYGCRESLVDGIRRGTDVMMAGKV"
BASE COUNT          299 a          640 c          715 g          251 t
ORIGIN      
        1 tcaccgagcg tctatggggc ctgctggtgc cgctgctgcc gcgctacgag gccgagggca
       61 agagctacct aacgatcgcg ctcggctgca cgggcggtcg ccaccggtcc gtctacctcg
      121 ccgagctgct ggccggccgg ctgcgcgacg cggggcgcga cgtggcggtg cggcaccgcg
      181 agctggccga gccgccgccg ccgccgccgc ggcccgccgg agatgcgcga tgatcggtct
      241 cgtcctcgtc acgcacgggc ggctcgccgc cgagttcgtg gcggcgctgg agcacgtggt
      301 gggcccgcag gagcgcgtgc tggcggtctg catcggcgct gacgacgata tcgaggcgcg
      361 ccgggcggag atcgtccgcg cgatccgcga tgtcgacgac ggcgccgggg tcgtggtgct
      421 gaccgacatg ttcggcggca cgccctcgaa cctcgccatc tcgaccatgg accgcaaggg
      481 cgtggaggtc atcgcgggcg tgaacctgcc gatgctgatc aagctggcca gcgtgcgcgc
      541 ggccgaaccc ctggcccagg ccgtggtggc ggcccaggag gcgggccgca agtacatcaa
      601 cgtcgcgagc cgcctgctcg cgcccgacaa gggctgagcg cgggtgtcga ccgggccggt
      661 cacccgcgag gtcacgatcg tgaaccagcg cggcctgcac gcccgagccg cggcgaagtt
      721 cgtgcgcgcc gccggggagt tcgaggccgc ggtcgcggtc acccgcgacg acaccaccgt
      781 cgccggcacc tcgatcctgg gcctgatgct gctcggcgcc ggcatcggca gcacgctcac
      841 gctgaaggcc gagggcacgg acgccgaggc cgcgatcgcg gcgatcgcgg acctggtcga
      901 gcgggggttc gacgagggct gacacgggcg gtcgagggtc gaggcgagag cgccgcctag
      961 ctggccagcc gctagcgaca cgacggctcg atctcgtcca agaagcttcc gacctcgccg
     1021 cgagccagaa cgctcgtcgc ccgctcggct tgtcgcattg cagcacctgt gcttaacgcg
     1081 tgccagcaga gccggctgag cgcctcggcg cgtcgtcgct cgaccctttc accgcggacc
     1141 tgacgaccat gacgagccag cataacgact acgccgtcgc cgacatcggg ctggcggact
     1201 gggggcggcg ggagatcgcg atcgccgaga ccgagatgcc ggggctgatg gcggtgcggg
     1261 cggagtatgg gccgtcgcag ccgctcaagg gggcgcgggt cgcgggctcc ctgcacatga
     1321 cgatccagac cgcggtgctg atcgagacgc tggttcatct cggtgccgac gtgcgctggg
     1381 cgtcgtgcaa catcttctcg acccaggacc acgcggcggc ggcgatcgcc aaggcgggcg
     1441 tgccggtgtt cgcctacaag ggtgagacgc tggacgagta ctgggactac gcgcaccgga
     1501 tcttcgactg gcacgacggc ggcgtgccga acatgatcct cgacgacggc ggcgacgcga
     1561 ccttgctggc gcacctcggg cggaaggcgg agcaggacgc ctcggtcctc gacgacccgc
     1621 acaacgagga ggaggaggcg ctctacaagt cgatgaagaa gcggctgaag agccatccgg
     1681 gctggtacac gagcctcggc gacaagatcc gcggggtgac cgaggagacg acgaccggcg
     1741 tgcaccggct ctacgacatg gagcgcaagg gcgagctgct gttcgcggcg atcaacgtca
     1801 acgacagcgt gacgaagtcg aagttcgaca acctgtacgg ctgccgcgag agcctggtcg
     1861 acggcatccg gcggggcacc gacgtgatga tggccggcaa ggtcg
//
LOCUS       DASWNJ010000751         2670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_296998, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000751 DASWNJ010000000
VERSION     DASWNJ010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296998"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="VGB85_12590"
     CDS             complement(<1..595)
                     /locus_tag="VGB85_12590"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0134920.1"
                     /translation="MSGPVHIDDRRPPHSVFVRHVDRPQWSAPPHAVAHGHAALAFYV
                     GGSAELEQRGRLRIGAGDVHIVPAGEAHRMTRAREVEVWCLGFDVVGLGPELAGLLAP
                     FERVRDGAAAVVAIPAGRQEHLRSLLAELRREAAAPGVAAEPVQRSLLTLALAEVARA
                     AGDHQPAAAPGLVARALRMIEQRCLGPLSLCDVAAALD"
     gene            complement(683..1096)
                     /locus_tag="VGB85_12595"
     CDS             complement(683..1096)
                     /locus_tag="VGB85_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134921.1"
                     /translation="MRPGLLAPLLLSLSLACTGRSAGPPGSGSGVSGAVDDAGNGSGN
                     NSGNGGGSGQESTPPADSSTPADPGTPPADPGAAGPACGDKTCAAGEQCISYYGIAGP
                     RGPKFQECGIPCPKGDCPEGKRCQTIADGPGPVCR"
     gene            complement(1131..>2670)
                     /locus_tag="VGB85_12600"
     CDS             complement(1131..>2670)
                     /locus_tag="VGB85_12600"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY0134922.1"
                     /translation="VVGDDDQAIYGWRGADVAQILGFPDEHPGCRMIRLEQNYRSTGH
                     ILTCADAIIRRNAGRLGKTLWSELGDGQQVKVRQLTDEHHEARVVVEQVLDALTEDIP
                     AHEIAVFYRTHAQSRVIEEALAGNAIRYAVYGGLSFFERKEIRDLIAYLRLLTNPGSD
                     VDFERIVNVPPRKIGDTTVGQLKGHAMERGISLMAALAELDQTGLSRAACSRLAGFAE
                     VMRLLVVAHAANMPLGELASEVLRVTGYREMLAAEQTEEATTRLENLQEFVGALAEFS
                     EDYPDQTLADYLEQVSLASDSDKKTDEARSVRLMTIHSAKGLEFTRVILTGMEEGVFP
                     HGRSLQDPSQMEEERRLAYVAVTRAKRDLLITHAARRTLYGQTQVNEPSRFLRDLPAA
                     ATDRGKRPGSSNYGSPYGAPARQEPSWNSDIVYDGDVDHVQPNSRAPQRRRPAPASAS
                     RAVHAAPADDDVALFVGMYFRHAKFGAGELIGWDGHGADLKLHLRFREVGTKVVLARF
                     CEPT"
BASE COUNT          349 a          938 c         1008 g          375 t
ORIGIN      
        1 ggtcgagcgc cgcggcgaca tcgcacagcg acagtgggcc gaggcagcgc tgctcgatca
       61 tccgcagcgc ccgggcgacc aggccgggcg ccgctgcggg ctggtggtcc ccggccgcgc
      121 gcgcgacctc ggcgagcgcg agcgtcagca ggctgcgctg cactggctcg gcggcgacgc
      181 cgggcgcggc ggcctcgcgc cgtaactcgg ccaacaggct ccgcaggtgc tcctggcgac
      241 ccgcggggat cgccaccacc gcggcggcgc cgtcacgcac gcgctcgaag ggcgcgagca
      301 gaccggcgag ctcggggccg aggccgacga cgtcgaagcc gaggcaccac acctcgacct
      361 cgcgcgcccg cgtcatgcga tgcgcctcac cggccggcac gatgtgcacg tcgccggcgc
      421 cgatgcgcag ccgcccgcgc tgctcgagct ccgcgctgcc gccgacgtag aacgcgaggg
      481 cggcgtggcc gtgcgcgacc gcgtggggcg gtgcggacca ctgcggccgg tcgacgtgcc
      541 gcacgaacac cgagtgtggc ggccggcggt cgtcgatgtg tacgggcccg gacatcgccg
      601 caagttaccg ccgcgaaccc cagggcgcga ctcctgcgtc cgcgcgtctc accacggccg
      661 tgtccgatcg ttcgccgaca gatcagcgac agaccgggcc ggggccgtcg gcgatcgtct
      721 ggcagcgctt gccctccggg cagtcgccct tcgggcacgg gatcccgcac tcctggaact
      781 tcgggccccg gggaccggcg atgccgtagt acgagatgca ctgctcgccc gcggcgcagg
      841 tcttgtcgcc gcaggcgggg ccggccgcgc cggggtcggc cggcggtgtg ccggggtcgg
      901 ccggtgtgct gctgtcggcc ggcggcgtgg actcctgacc ggagccaccg ccattgccgg
      961 agttgttgcc ggagccgttg cccgcgtcgt ccaccgcccc gctgacaccc gagcccgagc
     1021 ccggcgggcc cgcgctgcgg ccggtgcagg cgagcgagag agacaggagg aggggggcga
     1081 gcagaccggg acgcatcgcg cgcgaggcta ccacaggcgc gcctcggctc tcaggtcggc
     1141 tcgcagaagc gcgcgaggac caccttggtg ccgacctcgc gaaagcgcag gtgcagcttg
     1201 aggtccgcgc cgtgcccgtc ccagccgatc agctcgcccg cgccgaactt cgcgtggcga
     1261 aagtacatgc cgacgaacag cgcgacgtcg tcgtcggccg gcgcggcatg aacagcgcgg
     1321 gacgccgacg ccggcgcggg tcgccgacgc tgcggcgcgc gtgaattggg ctgcacgtgg
     1381 tcgacgtcgc cgtcgtacac gatgtcgctg ttccagctcg gctcctggcg cgccggggcg
     1441 ccgtagggcg agccgtagtt ggaggacccg ggccgcttgc cgcggtcggt cgccgcggcc
     1501 ggcaggtccc gcaggaagcg cgagggctcg ttgacctggg tctggccgta cagcgtgcgc
     1561 cgggcggcgt gggtgatcag caggtcgcgc ttggcccggg tcacggcgac gtacgcgagc
     1621 cggcgctcct cctccatctg cgacgggtct tgtagagagc ggccgtgggg gaacacgccc
     1681 tcctccatgc cggtgaggat cacgcgcgtg aactcgaggc ccttggcgct gtggatcgtc
     1741 atcagacgca cgctgcgggc ctcgtcggtc ttcttgtcgc tgtcgctcgc gaggctgacc
     1801 tgctcgaggt aatccgccag cgtctggtcg gggtagtcct cggaaaattc cgcgagcgcg
     1861 ccgacgaact cctgcaagtt ctcgaggcgg gtcgtcgcct cctcggtctg ctcggcggcc
     1921 agcatctcgc ggtagccggt gacgcgcagc acctcggagg cgagctcgcc gagcggcatg
     1981 ttggcggcgt gggcgacgac gagcaggcgc atcacctcgg cgaagccggc gaggcgcgag
     2041 caggccgcgc gcgacagccc cgtctggtcg agctcggcga gcgcggccat cagcgagatg
     2101 ccgcgctcca tcgcgtggcc cttgagctgg ccgacggtcg tatcgccgat ctttcgcggc
     2161 ggcacgttga cgatccgctc gaagtcgacg tcgctgccgg ggttggtcag caggcgcagg
     2221 taggcgatca ggtcgcggat ctccttgcgc tcgaagaagc tgaggccacc gtagacggcg
     2281 tagcggatcg cgttgccggc gagggcctcc tcgatcacgc gcgactgggc gtgcgtgcga
     2341 taaaaaacgg cgatctcgtg cgccgggatg tcctcggtca gggcgtcgag cacctgctcg
     2401 acgacgacgc gcgcctcgtg gtgctcgtcg gtcagctggc ggaccttgac ctgctggccg
     2461 tccccgagct cactccacag cgttttcccc aaacgcccgg cgttgcggcg gatgatcgcg
     2521 tcggcgcagg tgaggatgtg gccggtgctg cgatagttct gctcgaggcg gatcatcctg
     2581 cacccggggt gctcgtcggg gaagccgagg atctgcgcga cgtcggcgcc gcgccagccg
     2641 tagatcgcct ggtcgtcgtc gccgacgacg
//
LOCUS       DASWNJ010000752         5801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_579148, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000752 DASWNJ010000000
VERSION     DASWNJ010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579148"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="VGB85_12605"
     CDS             <1..1187
                     /locus_tag="VGB85_12605"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0134923.1"
                     /translation="FCRHTAAIYRQVSGPFIASAAPTLPGLVGSLGFSGLRGIFKIDF
                     HRSMWKALGDFFKDPRLRQLFGRYATYYGSDPFTAPATLNLIAHVEQAGVWGVEGGMI
                     EVARALERVARGLGVDVRCGAEVAEVMTRSGKAVGVLLAGGEVVAADAVIVNADVNAV
                     AQGRFGPAVAAAVKAPAVDRRSLSAVTWAAVAEARGFPLLRHNVCFSRDYAAEFADLR
                     AGRLPAEPTVYICAQDRGDTEDPGAGGAQRMLILVNAPPRGGEGRFASSEIDACQRTT
                     LQLLRRCGLDVTLDPSASSVTTPNEFAAMFPGTGGALYGAATRGWTDSLTRHAARTKL
                     SGLYLAGGSVHPGAGVPMATLSGRYAAQSVCEDLASTAGWRPVVTAGGTSTRSATTVA
                     SG"
     gene            1094..2032
                     /locus_tag="VGB85_12610"
     CDS             1094..2032
                     /locus_tag="VGB85_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134924.1"
                     /translation="MRGPRFDRGVAPGGYCWWYLDALSDDGRFGLTIIAFVGSVFSPY
                     YARARARSRWGIADPLEHCAINVALYGPGVDTWVLSEYGPDEVERGADHFHLGRSSVG
                     WEGDCLVVRFDERTALLGQRVAGVVRLYPSALPAAPVHIDHRARHTWWGVAPHAKVEA
                     TLTHPDLKLHGTGYHDSNFGDEPLEAAFLDWNWSRASLPDRAAVLYDARRVDGSVLRL
                     GRVFHADGRVEPLAAPRPVELGRTRWLLPRTTRTDGADAKVVRTLEDTPFYARTALQT
                     SLGGQRMAAVHESLSLERFCSPMVQTMLPFRIRRGG"
     gene            complement(2207..5524)
                     /locus_tag="VGB85_12615"
     CDS             complement(2207..5524)
                     /locus_tag="VGB85_12615"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0134925.1"
                     /translation="MASLHEPQTAVRPFGAESTSRPFGSEAADTTGWKPRSALVGTLL
                     ANRYRIVAPVGQGSTGAVFRADDIQLNKPVAVRVLVPTLVAQPGLIPRIRARIERNLA
                     IEQREPGALINLVDIFDVGMTIEGELFVVTDFIDGDHLSTMLTRGGRLPWSRARHLLV
                     RLCQILHGFHGHGIVLGALEARHCYAVRNKNKHEAIKVVSTAIFEQLASTIGPHAGHG
                     ATVLARYVAPEQACGEPIDARSDIYSFGVIAYELLTGSVPFTDANPVRLVAMHIQQPP
                     RPLRVAAPDAGIPAEVESILLRCLAKSPADRQESMDALANALLAIPESASNSAHANVT
                     ASLAASEIPAAPADLAAAATLAVSNRTSPHAEDTAHELPAAGVSAATLAVTNRTSPPA
                     DDTATAGVSPATLAVTNRTSPPADDTARELALAPGAAAATLAVTNLTSPPAEDSTVPV
                     AAPPPVAPEHHVPSTHTPPIEEPPASSPGSAGVAPATLAVTNKTSPPAEDSTVPVDAP
                     PRELDAPAAADAPQSVAHRPSAGVPRTQVTYPPGSLSADMIAGKAASPGFGPLRLPPR
                     PTLGGRPATLAPGSLGAPPTLGAPPPTGGVPISLPRPPTLGMAAATPAEPTPAPGPRL
                     PSAALPAGSVPAPEASRITPADSTSRGTAVAAGAALGSLGSLSAAVAAATADLAPAQP
                     VTAQPVPTDSSPQPVPTASSPVPADSLVAADTLARAPVADAPAAVRSVVHATDIRRAE
                     PASASGTYLRSALADAVESARPGPQASAQQPRVEPRVEPRVEARAEPRVEPRAEPQPV
                     VSEQALRGAALAAAPVPVGVTPAASLSQVTGTTDSLVGEVPRSRAGLYVVLGLAAAAG
                     IAALVFTSPSFNGPPTAPTGPQAAISQPAAPTKLAQATPPPGPTVAVDGATPERGAAV
                     IADPPETPEPDPIPEVQPTPPEPEPTADPEPTPGKKPRGEKPGTPRKKRPPVAEEPEE
                     PDVFDQLREHMAKKKAQEEEYAANLARSNAAAKPTPTPTPTPAPKEGQTDAEKAKETL
                     ERAKQAAGSGNPQLGYTLAKQSYGLARSQEALEVMGTCACRLKNEANARAAHVALSGS
                     PRRASVIEACTKTGITL"
BASE COUNT          641 a         1840 c         2493 g          827 t
ORIGIN      
        1 agttttgccg ccacaccgcg gccatctaca ggcaggtgtc gggtccgttc atcgcctcgg
       61 cggcgccgac cttgccgggc ctggtcgggt cgctcggctt ctccgggctg cgtgggatct
      121 tcaagatcga ctttcatcgc agcatgtgga aggcgctcgg cgattttttc aaagatccgc
      181 ggctgcggca gctgttcggg cgctacgcga cctactacgg cagcgatcct tttacggcgc
      241 ctgcgacctt gaacctgatc gcccacgtcg agcaggcggg ggtgtggggg gtcgagggcg
      301 ggatgatcga ggtggcgcgg gcgctcgagc gggtcgcccg cgggctcggc gtcgacgtgc
      361 gctgcggggc cgaggtcgcc gaggtgatga cgcgcagcgg caaggcggtc ggcgtgctgc
      421 tcgctggcgg cgaggtcgtc gcggccgacg cggtgatcgt caacgcggac gtcaacgcgg
      481 tcgcgcaggg gaggttcggc ccggcggtcg cggcggcggt gaaggccccg gcggtcgacc
      541 ggcggtcgct gtcggcggtc acgtgggcgg cggtcgccga ggcgcggggg ttcccgctgc
      601 tgcgccacaa cgtgtgtttt tcgcgggact acgcggccga gttcgcggac ctgcgggcgg
      661 gccggttgcc ggcggagccg acggtgtaca tctgcgccca ggaccgcggg gacacggagg
      721 accccggggc cgggggcgcg caacgtatgt taattctggt caacgcgccg ccacgcggcg
      781 gcgagggccg ctttgcttct tcggagatcg acgcatgtca acgcacgacc ctgcaactgc
      841 tgaggcgctg tggcctggac gtgacgctgg atccgtcggc gagctcggtg acgacgccga
      901 acgaattcgc ggcgatgttc ccgggcacgg gcggggcgct gtacggggcg gcgacgcggg
      961 gctggacgga ctcgctcacg cggcacgcgg cgcgcacgaa gctctcgggg ctctatctgg
     1021 cgggcggcag cgtgcacccc ggggcggggg tgccgatggc gaccttgagc gggcgatacg
     1081 cggcgcagag cgtatgcgag gacctcgctt cgaccgcggg gtggcgcccg gtggttactg
     1141 ctggtggtac ctcgacgcgc tcagcgacga cggtcgcttc gggctgacga tcatcgcgtt
     1201 cgtcggcagc gtgttctcgc cgtactacgc gcgggcgcgg gcccggtcgc gctgggggat
     1261 cgccgacccg ctcgagcact gtgcgatcaa cgtggcgctg tacgggcccg gagtggacac
     1321 gtgggtgctc tcggagtatg ggcccgacga ggtcgagcgc ggggccgatc actttcacct
     1381 cgggcgcagc agcgtggggt gggagggcga ctgcctggtc gttcgcttcg acgagcgcac
     1441 ggcgctgctc gggcagcggg tcgcgggggt ggtccggctg tatccctcgg ccttgcccgc
     1501 ggcgccggtg cacatcgatc accgcgcgcg gcacacctgg tggggcgtcg cgccgcacgc
     1561 caaggtcgag gcgacgctga cacacccgga cctcaagctg cacgggaccg gctaccacga
     1621 ctccaacttc ggcgacgagc cgctcgaggc ggcgttcctc gactggaact ggtcgcgcgc
     1681 gtcgctgccg gaccgcgcgg cggtgcttta tgacgcgcgg cgggtcgatg ggagcgtgct
     1741 gcggctcggg cgggtgtttc acgcggatgg tcgcgtcgag ccgctggcgg cgccgcgtcc
     1801 ggtcgagctc gggcgcacgc ggtggttgtt gccgcggacg acccggaccg acggcgccga
     1861 cgcgaaggtg gtgcgcacgc tcgaggacac gccgttttac gcgcgcaccg cgttgcagac
     1921 gagcctcggc gggcagcgca tggcggcggt gcacgagtcg ctgtcgctcg agcggttctg
     1981 ttcgccgatg gtgcagacga tgctgccgtt tcgcatccgc cgcggcgggt gagggggcgt
     2041 cagagcggcg ggcgcccggc gagcagcgag cgtgtcggcc ggcgccagcg ctgcgggtcg
     2101 agggtcaggc gcacgcgatc gcctcgaccg atgccgacac gcgacggacc tggtccgtcg
     2161 cgcgcgttat ttttaaaaaa taacctgtcc ctggggacag gtgggtctac agcgtgatgc
     2221 cggtcttggt gcaggcttcg atgacgctgg cgcggcgcgg ggagcccgag agggcgacgt
     2281 gggcggcgcg ggcgttggcc tcgttcttga ggcggcaggc gcaggtgccc atcacctcga
     2341 gggcctcctg cgagcgggcg aggccgtagc tctgcttggc gagcgtgtag ccgagctgcg
     2401 ggttgccgga tccggcggcc tgcttggcgc gctcgagcgt ctccttggcc ttctcggcgt
     2461 cggtctggcc ctccttgggc gcgggggtcg gcgtcggggt gggggtcggc ttggcggcgg
     2521 cgttggagcg ggcgaggttg gccgcgtact cctcctcctg ggccttcttc ttggccatgt
     2581 gctcgcgcag ctggtcgaac acgtccggct cctcgggctc ctccgcgacg gggggtcgct
     2641 tcttgcgcgg cgtgccgggc ttctctccgc ggggcttctt gccgggcgtc ggctcggggt
     2701 cggcggtcgg ctcgggctcg ggcggggtcg gctggacctc ggggatcggg tcgggctcgg
     2761 gggtctcggg cgggtcggcg atcacggcgg cgccgcgctc gggcgtcgcg ccgtcgacgg
     2821 cgacggtcgg gccgggcggg ggtgtcgcct gggcgagctt ggtcggggcg gccggctggg
     2881 agatcgcagc ctggggaccc gtgggggcgg tcggcggacc gttgaagctc gggctggtga
     2941 acacgagcgc ggcgatgccg gccgcggccg cgaggccgag cacgacgtac aggccggcgc
     3001 ggctgcgcgg gacctcgccg accagcgagt ccgtggtgcc ggtgacctgc gagagcgagg
     3061 cggccggggt gacgccgacc gggaccgggg cggcggccag cgccgcgccc cgcagggcct
     3121 gctccgacac gaccggctgc ggctcggcgc gtggctcgac cctgggctcg gcccgcgcct
     3181 cgaccctggg ctcgacccgt ggttcgaccc gtggctgctg cgccgatgcc tgcgggccgg
     3241 ggcgggccga ctcgacggcg tcggcgaggg cgctgcgcag gtaggtgccc gaggccgagg
     3301 cgggctcggc cctgcggatg tcggtcgcgt ggacgaccga gcgcactgcc gccggggcgt
     3361 cggcgaccgg ggcccgggcg agggtgtcgg cggcgaccag gctgtccgcc gggacaggcg
     3421 agctggccgt cgggacaggt tgcggcgagc tgtccgtcgg gacaggttgt gcggtgacag
     3481 gttgtgcggg ggcgaggtcc gcggtggccg cggcgacggc ggcggagagc gagccgagcg
     3541 agccgagcgc ggcgcctgca gcgaccgcgg tgccccggct cgtgctgtcc gcgggggtga
     3601 tgcgggaggc ctcgggggcc gggacgctgc cggcgggcag cgccgcggag ggcaggcgcg
     3661 ggccgggggc gggggtcggc tcggccgggg tcgcggcggc catgccgagg gtcggcggac
     3721 gcggcagcga gatcgggacg ccaccggtgg gaggcggggc gccgagggtc ggcggggcgc
     3781 cgaggctgcc cggggcgagc gtggccgggc ggccgccgag ggtcggtcgc gggggcaggc
     3841 gcagcggccc aaagccgggg ctcgccgcct tgccggcgat catgtccgcg gacagcgagc
     3901 ccggcggata ggtgacctgc gtgcgcggga cgccggcgga ggggcggtgg gcgaccgact
     3961 gcggggcgtc ggcggccgcg ggcgcgtcga gctcgcgcgg gggcgcgtcg accgggacgg
     4021 tcgagtcctc ggcgggcggg ctggtcttgt tggtgaccgc gagggtcgcc ggggcgacgc
     4081 cggcgctgcc gggcgagctc gcgggcggct cctcgatcgg tggggtgtgg gtgctgggga
     4141 cgtggtgttc gggggcgacg ggcgggggcg cggcgaccgg gacggtcgag tcctcggcgg
     4201 gcgggctggt gaggttggtg acggcgaggg tcgccgcggc ggcgccgggg gccagggcga
     4261 gctcgcgggc ggtgtcgtcg gcgggcgggc tggtgcggtt ggtgaccgcg agggtcgcgg
     4321 gcgagacgcc ggcggtcgcg gtgtcgtcgg cgggcgggct ggtgcggttg gtgacggcga
     4381 gggtcgcggc cgagacgccg gcggccggga gctcgtgggc ggtgtcctcg gcgtgcgggc
     4441 tggtgcggtt gctgaccgcc agcgtggccg cggcggcgag gtcggcgggc gcggccggga
     4501 tctcgctggc ggcgaggctc gcggtcacgt tggcgtgcgc gctgttcgag gccgactcgg
     4561 ggatcgccag cagcgcgttg gccagcgcgt ccatcgactc ctggcggtcg gcgggcgact
     4621 tggcgaggca gcgcagcagg atcgactcga cctccgcggg gatgccggcg tccggggccg
     4681 cgacgcgcag cggccggggc ggctgctgga tgtgcatcgc caccaggcgc accgggttgg
     4741 cgtcggtgaa gggcacgctg ccggtcagca gctcgtaggc gatcacgccg aacgagtaga
     4801 tgtcgctgcg cgcgtcgatc ggctcgccgc aggcctgctc gggcgcgacg tagcgggcga
     4861 gcacggtcgc gccgtgaccc gcgtgcgggc cgatcgtgct ggcgagctgc tcgaagatcg
     4921 cggtgctgac gaccttgatc gcctcgtgct tgttcttgtt gcggaccgcg tagcagtggc
     4981 gggcctcgag cgcgccgagc acgatgccgt ggccgtgaaa gccgtgcagg atctggcaca
     5041 ggcggaccag caggtggcgg gcgcgcgacc acggcaggcg cccgccgcgg gtcagcatcg
     5101 tcgagaggtg gtcgccgtcg atgaagtcgg tgacgacgaa cagctcgccc tcgatcgtca
     5161 tgccgacgtc gaagatgtcg acgaggttga tcagggcgcc cggctcgcgc tgctcgatcg
     5221 ccaggttgcg ctcgatgcgg gcgcggatgc ggggaatgag tcccggctgg gcgacgagcg
     5281 tcggcaccag cacgcggacg gcgaccggct tgttgagctg gatgtcgtcg gcccggaaca
     5341 ccgcgccggt cgagccctgc ccgacagggg cgacgatgcg ataacggttg gccaggagcg
     5401 tgccgaccag cgcggacctc ggcttccaac ccgtcgtatc cgcggcctcg gacccaaagg
     5461 gccgggaggt cgactctgcc ccgaagggcc gtacagccgt ttgcggctcg tggagcgacg
     5521 ccatttcgtg aacataccgc aatacgcgac agcccgcgac cgcgcgcgga cgcgcgacga
     5581 tccgggggcg cctgcccgca cacgtgggcg gtgtgccgca ggtgggccac aggagcgtcg
     5641 ccagcggtgc gctggcgcct gcgcacctgc gagtgaggag cgggcaggcg gagatcaggg
     5701 ggtcggcgag gcgagctcga cgtcggctgc cggcagcatc cgcgggtgcc acaggccctc
     5761 ggagcgggcg atgaaggcgg cggccgtgtg gtcgccgagc a
//
LOCUS       DASWNJ010000753         3171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_297023, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000753 DASWNJ010000000
VERSION     DASWNJ010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297023"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1501
                     /locus_tag="VGB85_12620"
     CDS             <1..1501
                     /locus_tag="VGB85_12620"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134926.1"
                     /translation="EQFPIASVKDLAARKILATEGLARMLSRQNYDGGFRFWDTPDRS
                     WPYLSAWATFALLEGKKAGFAVDEQALVRATGYLENFVRNGESTPWGRYYDHTSRAFA
                     LWLLSREDRGQELFDRVYAKRDEIPLYARAHLMAAAHKYGRASEREALLKDFRARVTE
                     TARTAHYAERTSEAEADGLQLLMHSDVQTDATAMMAMLDVTPDDPVLPKVMAGIMDDR
                     DPQKGGRWGTTHANAWALLAASRYFTTVEKEVPDYVARIWLDTTFAGEQAFKGRSMAV
                     VEQHVPMARLMQDDPKSVLLAKDGPGKLYYRLGLRYAPADFNMKAEEQGFTVYRSYEP
                     LGQGVDKPDPESVKRLPDGTYEVKAGALVRVNLTLVAKDRANFVVVDDPLPAGFEGQN
                     ARFATTLQEVNGGVVKQSVDHGGGLPWAALERGWWWWRPWWSFSHTEMRDDRMLLFAN
                     HLPAGVYTYSYTARATTIGEFQLPPVHAEAMYMPELFGHSASSKVRVVE"
     gene            complement(1657..3096)
                     /locus_tag="VGB85_12625"
     CDS             complement(1657..3096)
                     /locus_tag="VGB85_12625"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEY0134927.1"
                     /translation="MMMRTWAQDEFGDTALGDSRRTKRLVTMMSRLEVNPGGPVARVY
                     PKGPERQAAYKWLENPFVRVAALERARNVACMRRMAGLCGTFAVPVDKTSIHLSDRLG
                     ERDFGSVGNRRLGSRGVQVITGLALDATGAPLGVAHQMLWARSETPSPPRRPGHKSRK
                     RERDRRPPAERESMRWVEVLAEIHGLGQEHAPDARLWFQLDREGDFWGVHKLAAELGA
                     WVTVRMNKSHVVRDARGVPAKLMDWVRKQPIEHWIELTLPAHDGRPQRRARLSLRFAK
                     AQLRLPIAGEHRWVTKTFVYVDEPNRASAEDRIQWLLSTTRPVDSITDALAVIDNYKR
                     RWRIEDFHRTWKSGCCNIEASQLQSTSVFHRWAILTSSMAARAEYIKHYAREEPDGPA
                     TLVFTQEEIDTVVTYRRIESPKARIQYNPGEVPKLSEMTRWVAEFGGFMKSSKVAPGA
                     VTIARGLAYLEPLVLGRRLALQQMDDNSG"
BASE COUNT          514 a         1126 c         1045 g          486 t
ORIGIN      
        1 cgagcagttc ccgatcgcgt cggtcaagga cctcgcggcg cgcaagatcc tcgcgaccga
       61 gggcctcgcg cgcatgctct cgcgccagaa ctacgacggc gggttccgct tctgggacac
      121 accggatcgc agctggccgt acctctcggc gtgggcgacc ttcgccctgc tcgagggcaa
      181 aaaggccggc ttcgccgtcg acgagcaggc gctcgtgcgc gcgaccggct acctcgagaa
      241 cttcgtccgc aacggcgagt cgaccccgtg gggtcgctac tacgaccaca ccagccgggc
      301 cttcgcgctg tggctgctca gccgcgagga ccgcgggcag gagctgttcg accgcgtgta
      361 cgccaagcgc gacgagatcc cgctgtacgc ccgcgcgcac ttgatggccg ccgcgcacaa
      421 gtacggccgc gccagcgagc gtgaggcgct gctcaaggac ttccgcgcgc gcgtcaccga
      481 gaccgcgcgc accgcccact acgccgagcg caccagcgag gccgaggccg acggcctgca
      541 gctgctcatg cactccgacg tgcagaccga cgcgaccgcg atgatggcga tgctcgacgt
      601 gacgcccgac gaccccgtgc tgccgaaggt gatggccggg atcatggacg accgcgaccc
      661 gcaaaagggt ggccgctggg gcacgaccca cgccaacgcc tgggccctgc tcgccgccag
      721 ccgctacttc acgaccgtcg agaaggaggt gcccgactac gtcgcccgca tctggctcga
      781 cacgaccttc gccggcgaac aggcgttcaa gggccgcagc atggcggtcg tcgaacagca
      841 cgtgccgatg gcccgcctga tgcaggacga cccgaagtcc gtgctgctcg ccaaggacgg
      901 ccccggcaag ctctactacc gcctcggcct gcgctacgcg cccgccgact tcaacatgaa
      961 ggcggaggag cagggcttca ccgtctatcg cagctacgaa ccactcggcc agggcgtcga
     1021 caagcccgac cccgagtcgg tcaagcgcct ccccgacggc acctacgagg tcaaggccgg
     1081 cgcgctggtc cgcgtgaacc tgacgctggt cgccaaggac cgcgccaact tcgtcgtcgt
     1141 cgacgacccg ctgcccgccg gcttcgaggg gcaaaacgcc cgcttcgcca cgaccctgca
     1201 ggaggtcaac ggcggcgtgg tcaaacagtc ggtcgaccac ggcggcggcc tgccctgggc
     1261 cgccctcgag cgcggctggt ggtggtggcg cccctggtgg agcttctccc acaccgagat
     1321 gcgtgacgac cgcatgctcc tgttcgccaa ccacctgccc gcgggcgtct acacgtacag
     1381 ctacacggcg cgggcgacga cgatcggcga gttccagctg ccgccggtgc acgcggaggc
     1441 gatgtatatg ccagagctgt tcgggcacag cgcgagcagc aaggtgcgcg tggtcgaata
     1501 gggggccggg ctggccgggc tcgctcgctc gcgagtccgg ggacgcccgc tcgcgagtcc
     1561 ggggacgccc gctcgcgtgt ccggggacgc ggggcgaggc tacatgggcc ggggagcggg
     1621 ggccgccgga cggcgggtcc ctgcgacgcc tggccgttag ccggaattat cgtccatttg
     1681 ctgcaaggcg agtcgacggc cgagcacgag tggttcgagg taagcgaggc cgcgggcgat
     1741 ggtgacggcg ccgggtgcga ccttgctgga cttcatgaag ccgccgaact cggcgaccca
     1801 tcgggtcatc tcggagagct tggggacctc gccggggttg tactggatcc tcgctttggg
     1861 cgactcgatg cggcgatagg tcaccacggt gtcgatctct tcctgcgtga agacgagggt
     1921 cgcgggaccg tcgggctcct cgcgcgcgta gtgcttgatg tattcggcgc gggcggccat
     1981 ggaggacgtg aggatagccc agcggtgaaa gacggaagtc gactggagct gcgacgcctc
     2041 gatgttgcag cagccggact tccaggtgcg atggaagtcc tcgatgcgcc agcgccgctt
     2101 gtagttgtca atgaccgcga gggcatcggt gatgctgtcg accggacgtg tcgtgctcag
     2161 cagccactgg atgcggtcct cggcgctggc ccgattgggc tcgtcgacat agacgaacgt
     2221 cttggtgacc caccgatgct ctccagcgat cggcagtcga agctgcgcct tggcgaagcg
     2281 caggctcagg cgtgcccttc gctgcggtcg gccgtcgtgt gccggcaagg tgagctcgat
     2341 ccagtgctcg atcggctgct tgcggaccca gtccatcagc ttcgccggaa cgcctcttgc
     2401 gtcgcggacg acatggctct tgttcatccg cacggtcacc caagcgccga gctcggcggc
     2461 cagcttgtgc acgccccaaa agtcgccctc gcggtcgagc tgaaaccaca accgggcgtc
     2521 gggcgcatgc tcctggccca gcccgtggat ctcggcgagc acctcgaccc atcgcatcga
     2581 ctcacgctcg gcgggcggac gccggtcgcg ctcgcgcttg cggctcttgt gtcccggacg
     2641 ccgcggcggg ctcggcgtct cacttcgagc ccacaacatc tggtgcgcca cccccagtgg
     2701 agcgcccgta gcgtccagcg ccagacccgt gatcacctgc acgccacgcg acccaaggcg
     2761 tcggttgccc accgacccga agtctcgctc gcccaaccga tccgacagat ggatgctcgt
     2821 cttgtccacc ggaaccgcga acgtcccgca cagccccgcc atccgtcgca tgcacgccac
     2881 attgcgtgcc cgctccagcg ccgccacgcg tacaaagggg ttctccagcc acttatacgc
     2941 tgcctgacgc tccggtccct tcggatacac ccgtgcgacc gggccgccgg gattcacttc
     3001 gagacgcgac atcatcgtaa ccaggcgctt ggtccgccga gagtctccca gcgctgtgtc
     3061 accgaactca tcctgagccc atgtacgcat catcatgatc caactacctt gatgacgcgc
     3121 tcgcgccgcc caaccccctt gtcggcgtca aaatttcaga tcaacggcca g
//
LOCUS       DASWNJ010000754         2134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_297030, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000754 DASWNJ010000000
VERSION     DASWNJ010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297030"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(257..715)
                     /locus_tag="VGB85_12630"
     CDS             complement(257..715)
                     /locus_tag="VGB85_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134928.1"
                     /translation="MTTDSLTHPLPFFHHAATARVLYIASGPCPLVFSISLKDIGRHA
                     PVVNHGPVLHKTTHPFGESTTVASLAGDAVRRELCDATPLAAGALDETLSLWANFCDD
                     VADRLNDGDPLSEALDDTWVLGDGHDVELDDEPAVPPSTRGDSCVVGSRQ"
     gene            complement(723..1052)
                     /locus_tag="VGB85_12635"
     CDS             complement(723..1052)
                     /locus_tag="VGB85_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134929.1"
                     /translation="MDDTDANKPRHREAAHRPRSPRATGRATTTPRIIPASALAPGLA
                     TIIVSLPGRTIRSRVFMVQVQPHGSVQVAFASGDMITLSPRTMVGSIERPAPPRRPRP
                     PRLSNAS"
     gene            1203..1541
                     /locus_tag="VGB85_12640"
     CDS             1203..1541
                     /locus_tag="VGB85_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY0134930.1"
                     /translation="MTSNRSLDPAFVSTDDIMAAAGVARRTVSVWVEMGFLPPPIKVS
                     LGIRGVFNRFPAHAAERARFIAAKRAGGFTYDEVRAMLAEMDAQEARKAKAAARSQPA
                     PAAPKSGRRR"
     gene            1578..1928
                     /locus_tag="VGB85_12645"
     CDS             1578..1928
                     /locus_tag="VGB85_12645"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY0134931.1"
                     /translation="MTDRRRLDPAFATMADIMEAAGMTRRTIRVWIERGLLTKPVTIS
                     QGYPGGMFNRFPAHVLDQARFIVAMREQGLSLDEIAGLIAARDWTRAGRPSRAGVGSR
                     DDAPAPDRRAKRRP"
     gene            1885..>2134
                     /locus_tag="VGB85_12650"
     CDS             1885..>2134
                     /locus_tag="VGB85_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134932.1"
                     /translation="MMRLRQIAVLSAALELMPGCGGDVASSGFTSGPHPITSAAGSSG
                     SSDSTTSTSSSGSTSGEDSGSAAASGTTTGNMSTLDMGP"
BASE COUNT          301 a          716 c          790 g          327 t
ORIGIN      
        1 ggccagggcg cgctcgagag tgttgttgag agctgccagt gcgatcatcg tgtctcctcg
       61 tgtgggtggt gttgcttctg gaattcagcg gcaggctgcg ggcgggcgct ggctcggcgg
      121 tgcgagcgag cgccgccgcg aggccaacga gaaaagcgcg cgaggccggc ctcgacgtcg
      181 tactccgtgc gatggctggc ggccccgagg tcagctcgca gcgtggcccc gatcgtcggg
      241 tcataaagca gccgagtcac tgccggctcc cgacgacgca cgagtcgccg cgcgtgctcg
      301 gtggcaccgc cggctcgtcg tccagctcga cgtcgtggcc atcgcctagc acccacgtgt
      361 cgtccagcgc ttccgagagc gggtcgccgt cgttgagccg atcagcgacg tcgtcgcaga
      421 agttcgccca cagcgacagc gtctcgtcca gcgcgccggc tgcaagcggc gtcgcgtcgc
      481 acagctcgcg gcgcacggcg tcgccagcga gcgaggcaac tgtggtcgac tcgccgaacg
      541 ggtgggtggt cttgtgaagc accggcccgt ggttcaccac gggcgcatgc ctgccgatgt
      601 ccttgagaga gatcgagaac accagcgggc acgggccgga ggcgatgtac aggacgcggg
      661 cggtcgccgc gtggtgaaag aatgggagcg ggtgagtgag ggagtccgtg gtcatggtgc
      721 cctcagctcg cgttgctcaa gcgcggcggt cgtggccggc gcggtggggc cggacgttcg
      781 atgctgccga ccatggtccg gggcgagagt gtgatcatgt cgcccgaggc gaacgcgacc
      841 tgcacggagc cgtgcggctg gacctgcacc atgaacacgc ggctgcggat cgtgcggcca
      901 ggcagcgaga cgatgatcgt cgccagccct ggcgcgagcg ccgatgcggg gatgatgcgc
      961 ggggtcgtcg tggcgcgccc cgttgctctc ggcgagcgtg ggcggtgagc ggcctcgcgg
     1021 tgtcgcggtt tgttggcgtc ggtgtcgtcc atttgcggat actccttttt tattgttctc
     1081 tcactggcca cctcgacgct acactcgagg ctgacagcgg cgcaaggggg tgtttgagcg
     1141 ccgtttcggc gtctggctgg cgctggcgcg cgagcgcgcg ggtcccttat actcggcggg
     1201 cagtgaccag taaccgcagc ctcgaccccg cgttcgtgtc gaccgacgac atcatggccg
     1261 ccgccggcgt cgccaggcgg accgtgtcgg tgtgggtcga gatggggttc ctaccgccgc
     1321 cgatcaaggt gtcgctcggc atccgcgggg tgttcaaccg cttcccggcc cacgccgcag
     1381 aacgggcgcg cttcatcgcg gcgaagcgcg cgggcggctt cacctacgac gaggtccggg
     1441 cgatgctcgc ggagatggac gcgcaggagg cccgcaaggc caaagccgcg gcccgctcgc
     1501 agccggcgcc ggccgcccct aagagcgggc gccgccggta gcgcgccccc ctcggggccg
     1561 cgtaccatgc tgcagccatg accgaccgcc gccggctcga ccccgcgttc gccacgatgg
     1621 cggacatcat ggaggccgcg ggcatgaccc gccggacgat ccgcgtgtgg atcgagcggg
     1681 gactgctgac caagccggtc acgatctcgc agggctaccc cggcgggatg ttcaaccgct
     1741 tcccggcgca cgtgctggac caggctcgct tcatcgtcgc tatgcgtgag cagggcctgt
     1801 ccctcgacga gatcgccggc ctcatcgccg cccgggactg gacccgggct ggccgtcctt
     1861 cccgggctgg agtaggatcg cgcgatgatg cgcctgcgcc agatcgccgt gctaagcgcc
     1921 gcccttgagc tgatgccggg ctgcggcggc gacgtggcct cgtccggctt cacgagcggg
     1981 ccgcacccga tcacgtctgc cgccggcagc tcgggcagct ccgacagcac gacgagcacc
     2041 agcagctccg gtagcaccag cggcgaggac agcggcagcg cggccgcgag cggtacgacc
     2101 accggcaaca tgtcgacgct ggacatgggg ccga
//
LOCUS       DASWNJ010000755         1965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_439359, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000755 DASWNJ010000000
VERSION     DASWNJ010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439359"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1566)
                     /locus_tag="VGB85_12655"
     CDS             complement(<1..1566)
                     /locus_tag="VGB85_12655"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0134933.1"
                     /translation="MTRSKPIVTCTPVLQRSQVALRCITALAAVTAIPTARADAPASP
                     SSGAAHAPSDMSRGTGPSAPAAGPAAQPAARPVPSNMSQGTGPSAQPAAQPVPSNTTP
                     VPATDAPARCQDRRDVAWIRRCLPARNTWELGVFAGVTFPASHSHELFNAYAQRDARR
                     MGEEFWRPYRAAAPAFGLRAAYFPLSFLGGELEGGVMPTRVLEDGAPGARATLFSLRA
                     HLVGQVPLWRVTPFVVWGSGGIGTTGALGSDFDPMTHFGAGAKVFLTQRTLLRIDVRD
                     NVAAKKEVDAGATHYPEVLLGFSAVLGRKSPARTPAKDSDGDGFLDRSDACPFEPGRA
                     PDGCPERDRDDDGFLDSQDACPDVPGIAPDGCPEKDRDGDGFLDSLDQCPDVPGVAPD
                     GCPIPDTDGDGYLDNADSCVDKPETWNGYLDADGCPDDIPRQIPRGVIRGIYFDLDRD
                     TIKPRSRPVLDRAVAIFTEFPDVRVSISGHTDSTGSAAYNRDLSRRRAAAVKRYLVEH
                     GIDASRIETRGAGP"
     gene            complement(1645..>1965)
                     /locus_tag="VGB85_12660"
     CDS             complement(1645..>1965)
                     /locus_tag="VGB85_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEY0134934.1"
                     /translation="LLLTTSSDGTARLWRPRGDGHISLVLPQRGTATPGEPDHTLWTG
                     SFSPDGRHVLTAGADGLGRVFPVDLAAQVEEACARAGRNFDPATWKQLVGERPYQPTC
                     PDPE"
BASE COUNT          251 a          621 c          824 g          269 t
ORIGIN      
        1 ggggccggcg ccgcgggtct cgatgcggga cgcgtcgatg ccgtgctcga cgaggtagcg
       61 cttgaccgcg gcggcgcggc gccgcgacag gtcgcggttg taggccgcgg agccggtgct
      121 gtcggtgtga ccggagatcg agacgcgcac gtcggggaac tcggtgaaga tcgccacggc
      181 gcggtcgagc accgggcgcg agcgcggctt gatcgtgtcg cggtcgaggt cgaagtagat
      241 gccacggatg acgccacggg ggatctggcg cgggatgtcg tcggggcagc cgtcggcgtc
      301 gaggtagccg ttccaggtct cgggcttgtc gacgcaacta tcggcgttgt cgaggtaacc
      361 atcaccgtcg gtgtcgggga tcgggcagcc gtcgggcgcg acgccgggca cgtcggggca
      421 ttgatcgagc gagtcgagga agccgtcgcc gtcgcggtcc ttctcggggc agccgtcagg
      481 ggcgatgccg ggcacatcgg ggcaggcgtc ctggctgtcg aggaagccgt cgtcgtcgcg
      541 gtcgcgctcg ggacaaccat cgggcgcgcg gccgggctcg aaggggcacg cgtcgctgcg
      601 gtcgaggaag ccgtcgccgt cgctgtcctt tgcgggcgta cgcgccggtg acttgcggcc
      661 gagcacggcc gagaagccga gcaggacctc cgggtagtgc gtggccccgg cgtcgacctc
      721 cttcttcgcg gcgacgttgt cgcggacgtc gatccgcagc agggtgcgct gggtgaggaa
      781 caccttggcg ccggcgccga agtgggtcat cgggtcgaag tcgctgccga gcgcgccggt
      841 cgtgccgatg ccgccgctac cccacacgac aaacggcgtg acgcgccaca acgggacctg
      901 gccgacgagg tgcgcgcgca ggctgaagag ggtcgcgcgg gccccggggg cgccgtcctc
      961 gagcacgcgc gtcggcatca cgccgccctc gagctcgccg ccgaggaagc tcagcgggaa
     1021 gtaggcggcg cgcaggccaa aggcgggggc ggccgcgcga tacggccgcc agaactcctc
     1081 gcccatgcgc cgggcgtcgc gctgcgcgta ggcgttgaac agctcgtgcg agtgcgacgc
     1141 cgggaaggtg acgcccgcga acacgccgag ctcccaggtg ttgcgcgccg gcaggcagcg
     1201 acggatccac gcgacgtcgc ggcggtcctg gcagcgggcg ggggcgtcgg tcgccgggac
     1261 tggcgtggta ttcgaaggga ccggttgcgc ggcgggttgg gctgaaggac cggtcccctg
     1321 agacatgttc gaagggaccg gtcgcgcggc gggctgggcc gcggggccgg cggccggcgc
     1381 cgaaggaccg gtccctcggg acatgtccga cggggcatgt gccgcgcctg acgaggggct
     1441 cgcgggtgcg tccgcgcggg cggtggggat cgccgtcacg gcggcgaggg cggtgatgca
     1501 gcgcagcgcg acctggctgc gctgcaggac gggcgtgcag gtgacgatgg gcttgcttcg
     1561 ggtcattgcg cccggtgaag taccgacccc cctgcggtcg atcggtcgtc gatcgatcaa
     1621 tttttcggcg cgcggccgag cgcgtcattc ggggtcgggg caggtcggct ggtacgggcg
     1681 ctcgccgacg agctgcttcc aggtcgcggg gtcgaagttt cggccggcgc gggcgcaggc
     1741 ctcctccacc tgcgcggcga ggtcgaccgg gaacacgcgg ccgaggccgt cggcgccggc
     1801 ggtgagcacg tggcggccgt cggggctgaa gctcccggtc cacagcgtgt ggtcgggctc
     1861 gccaggcgtg gcggtgccgc gctgtgggag gacgaggctg atgtggccgt cgccgcgggg
     1921 gcgccacagg cgggcggtgc cgtcggagga agtcgtcagc agcag
//
LOCUS       DASWNJ010000756         2560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_507128, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000756 DASWNJ010000000
VERSION     DASWNJ010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507128"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2147)
                     /locus_tag="VGB85_12665"
     CDS             complement(<1..2147)
                     /locus_tag="VGB85_12665"
                     /inference="COORDINATES: protein motif:HMM:NF025689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein IcmF/TssM N-terminal
                     domain-containing protein"
                     /protein_id="HEY0134935.1"
                     /translation="MRNFVAFAALIAVIVGLVVGAVSMLGLPQIYATVAIGIGALLIA
                     IRAVVEALQRFGPQGPAGASPEADARRAARLLMLQRRQVLRRRLRGFVRALRAAQEPP
                     RLRYRERRGLPWWLVLGPQAHGKSALLAAAPGTREVEPGEPGEPRFFITADAAFVEVP
                     PDYHLRWSETALALAELLRGIRRARPRQPICGVLITARADLLLTNTEATLALLDAVRQ
                     QYNLLALSLAAQVPVDLVVTQIDRIAGFAELTAGLPSTGGVLGVTLPPREGKVSVQAA
                     VRERLAAAEGVLDWIRQRCHALVTRAEPGEGKQARLYGLWQQVDRLAEQVAVAAGRLA
                     GLPLPGGDPLRVRGVYFTCAQPDVVAPADEWMAALAQRVGGSLPLADAGVTVAPAFTT
                     ALFTDELPRDGQYADRLRALHRRRFTHAAAVALTAVALTAVSVQGMTLSAQANLDLMQ
                     RTLDSAYAVAVPTDTPQVAALSSLDDLRLAAVTWGEAAPEGYGWGLFRGDELAAATSD
                     AFRGAVCRGVFAPAAERSAQPLREFVARYAGGGLAVGREYQKKLEHLRQYLLLSDPPE
                     SIEPSLWSADTPSWREQLITTWGDLDGGAGDGRREKILQAQLALVGAAAGAPIAGADV
                     CARTGGGRAVARDPALVAAVREILLRTPQNRAMVDKLVAEIHRRKDIPSVHIHQLTTA
                     THIHDDVEVAPGFTRPGWLAFQAALDDLRERGGG"
     gene            complement(2154..>2560)
                     /locus_tag="VGB85_12670"
     CDS             complement(2154..>2560)
                     /locus_tag="VGB85_12670"
                     /inference="COORDINATES: protein motif:HMM:NF021377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HEY0134936.1"
                     /translation="GTTIAGHTFFAELAQTIDSRVASEPEAAVLEAYAVCLAFGFRGK
                     YEAHDIEGYEAMRARLNDKLRRSLAVPVELPAAAAVPWPRLRSLHAPLLWIAGLAVLF
                     AVAMLLTYHFGLAVDASLLRQRLIDLLGTRPT"
BASE COUNT          367 a          863 c         1038 g          292 t
ORIGIN      
        1 ccgccgccgc gctcgcgcag gtcgtcgagc gcggcctgga aggcgagcca gcccggacgg
       61 gtgaagccgg gggcgacctc gacgtcgtcg tggatgtggg tcgcggtcgt cagctggtgg
      121 atgtgcaccg aagggatgtc cttgcggcga tggatctcgg cgacgagctt gtcgaccatg
      181 gcgcggttct gcggggtgcg cagcaagatc tcgcggaccg cggcgacgag cgccggatcg
      241 cgggcgaccg cccggccacc gccggtgcgg gcgcacacgt cggcgccggc gatcggcgcc
      301 cccgcggcag cgccgacgag cgcgagctgt gcctgcaaga tcttctcgcg gcgaccgtcg
      361 ccggcaccac cgtcgaggtc cccccaggtg gtgatcagct gctcacgcca cgacggcgtg
      421 tccgcgctcc acaggctcgg ctcgatcgac tccggcgggt ccgacagcag gaggtactgg
      481 cggagatgct cgagcttctt ttggtactca cgaccgacgg cgaggccacc cccggcgtag
      541 cgcgcgacga actcacgcag cggctgggcg ctgcgctcgg cggccggggc aaacacgccg
      601 cgacagacgg cgccccgaaa cgcgtcggag gtggcggccg cgagctcgtc gccgcgaaac
      661 aggccccagc cgtagccctc cggggcggcc tcgccccagg tgacggcggc gaggcgcagg
      721 tcgtcgaggc tggagagggc cgccacctgc ggggtgtcgg tggggaccgc gacggcgtac
      781 gcgctgtcga gggtgcgctg catgaggtcg aggttggcct gcgccgagag ggtcatgccc
      841 tgcacgctga cggccgtcag cgcgaccgcc gtcagcgcga cggcggcggc gtgggtgaac
      901 cggcgccgat ggagggcgcg caggcggtcg gcgtactggc cgtcgcgcgg gagctcgtcg
      961 gtgaagaggg cggtggtgaa cgcgggggcc acggtgacgc cggcgtcggc gagcggcagg
     1021 ctgccaccga cgcgctgggc gagcgcggcc atccactcat cggcgggggc gacgacatcg
     1081 ggctgggcgc aggtgaagta gacgccgcgc acacgcagcg ggtctccgcc gggcaagggc
     1141 agccccgcga ggcggcccgc cgcgacggcg acctgctcgg cgaggcggtc gacctgctgc
     1201 cagaggccgt agaggcgcgc ctgtttgccc tcaccgggtt cggcgcgggt gaccagggca
     1261 tgacagcgct ggcggatcca gtcgagcacg ccctcggcgg cggcgaggcg ctcacgcacg
     1321 gcggcctgca ccgagacctt gccctcgcgc ggcggcaggg tgacaccgag gaccccgccg
     1381 gtcgacggca gcccggcggt cagctccgcg aagccggcga tgcggtcgat ctgggtgacg
     1441 acgaggtcga cgggtacctg cgccgcgagc gagagggcga gcaggttgta ttgctggcgg
     1501 accgcgtcga gcagcgcgag ggtggcctcg gtgttggtca gcaggaggtc ggcccgcgcc
     1561 gtgatcaaca cgccgcagat cggctggcgc gggcgggcgc gacggatgcc gcgcagcagc
     1621 tcggccagcg cgagggccgt ctcggaccac cggaggtgat aatccggcgg gacctcgacg
     1681 aacgcagcgt cggcggtgat gaaaaagcgc ggctccccgg gctcgccggg ctcgacctcg
     1741 cgggtgcccg gcgcggctgc gagcagcgcc gacttgccgt gcgcctgcgg tccgagcacc
     1801 agccaccacg gcaggccgcg acgctcgcgg tagcgcagcc gcggcggctc ctgggcggcc
     1861 ctgagcgcgc gcacgaagcc gcggagccgt cgacgcagca cctgccggcg ctggagcatc
     1921 aggagccggg cggcccgcct cgcgtcggcc tcgggggagg cgcccgccgg gccctggggg
     1981 ccgaagcgct gcagcgcctc gacgaccgcc cggatcgcga tcagcagggc gccgatgccg
     2041 atggcgaccg tggcgtagat ctgcggcagc cccagcatgg agacggcgcc gacgacgagg
     2101 ccgacgatga cagcgatgag ggcggcgaag gcgacgaagt tacgcatggt ggctcaggtg
     2161 gggcgcgtgc cgagtagatc gatgagacgc tggcgcagca gcgatgcgtc gaccgcgagc
     2221 ccgaagtggt aggtcaggag catcgcgacc gcgaacagca cggcgaggcc ggcgatccac
     2281 agcagcgggg cgtgcaggga ccgcagccgt ggccagggga ccgcagccgc ggcgggcagc
     2341 tcgacgggga ccgcgagcga gcgacgcagc ttgtcgttga ggcgcgcgcg catggcctcg
     2401 tagccctcga tgtcgtgggc ctcgtacttg ccgcggaatc cgaacgcgag acacacggcg
     2461 taggcctcga gcacggcggc ctcgggctcg ctggcgacgc ggctgtcgat cgtctgggcg
     2521 agctcggcga agaaggtatg gccggcgatc gtggtgccgt
//
LOCUS       DASWNJ010000757         1962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_14443, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000757 DASWNJ010000000
VERSION     DASWNJ010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14443"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VGB85_12675"
     CDS             <1..693
                     /locus_tag="VGB85_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134937.1"
                     /translation="LSADMRELLVLTLRACRRVLVCASGGVNLDRFMRALMGEIPDSL
                     RVACISDSGRLGSSRKGWVPVRRLIEPGDKIELQHVLNVLLRGGLDLVVSQQCNHSDA
                     AAVIDALAGASRGVVVSLWGINTAHALSRLAALGTASGGAVQGLALSLAHAVDIVVRL
                     NSGVGHESMQIVEIVEPDTRADNTIEYRPLFVAARAEDGTTEFRSTPNVAGFLRRLAD
                     QGADVPQRLLNL"
     gene            850..1659
                     /locus_tag="VGB85_12680"
     CDS             850..1659
                     /locus_tag="VGB85_12680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0134938.1"
                     /translation="MRLLLLITLATLACGAPAPAPTPTPTPAQKPAEHASGPKEHAHD
                     ALPPGFHAQEHEIAEEMAQPAADHRPRGHHKFHRFDRAEDWVAQFDAADRAEWQKPDA
                     VIAALALAPDASVADLGAGTGYFAVRLARAVAKGRVYAEDLEPDMVRYLGERAAKEGL
                     GNITAVQGVADDPKLPAPVDVAIMVDSYHHIAEPTAFFARVRDSLRPGGLLVIVDFKK
                     DAPDDAPGPPAAMRVADEIVTAHLRKLGYVHERTDRDLLPYQYLVFMRRPK"
     gene            1764..>1962
                     /locus_tag="VGB85_12685"
     CDS             1764..>1962
                     /locus_tag="VGB85_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134939.1"
                     /translation="MNFDVPRHIGAVTRVVDNRQRDGRPTRVVVATRTYDSDIADVWD
                     AITSAERIPRWFLPVSGELRLG"
BASE COUNT          277 a          758 c          646 g          281 t
ORIGIN      
        1 ctcagcgccg acatgcgcga gctgctggtc ctgaccctgc gcgcctgccg gcgcgtgctc
       61 gtctgcgcct cgggcggcgt caacctcgac cgcttcatgc gcgccctgat gggcgagatc
      121 cccgattcgc tgcgcgtcgc ctgcatctcc gactccggcc gcctcggcag cagccgaaag
      181 ggctgggtcc cggtccgccg cctgatcgag cccggcgaca agatcgagct gcagcacgtc
      241 ctcaacgtgc tcctgcgcgg cggcctcgac ctcgtcgtct cgcagcagtg caaccacagc
      301 gacgcggccg cggtgatcga cgcgctcgcc ggggccagcc gcggcgtcgt cgtctcgctg
      361 tggggcatca acacggccca cgccctctcg cgcctcgcgg cgctcggcac cgcctcgggc
      421 ggcgcggtcc agggcctcgc cctctcgctc gcgcacgcgg tcgacatcgt cgtccgcctc
      481 aacagcggcg tcggccacga gtcgatgcag atcgtcgaga tcgtcgagcc cgacacccgc
      541 gccgacaaca cgatcgagta ccgcccgctg ttcgtcgcgg cccgggccga ggacggcacc
      601 accgagtttc gcagcacccc gaacgtcgcc ggcttcctgc gccgtctcgc cgatcagggc
      661 gccgacgtgc cccagcggct gctcaacctg tgacggcctg acccggcccg ccccatgttc
      721 gtggcgcgag gccgggtgtc gcatgactcc cggacatcgc atcgcgcgat cgggcggcga
      781 gcaccgtctc cgggcctcgc ctggccacgt ctccctgcct tgccagccat gactcgatcg
      841 gtacccctca tgcgactgct gctcctgatc acactggcga ccctcgcgtg tggcgccccg
      901 gcgccggcac ccacgcccac gcctacgccc gcgcagaagc cggcggagca cgcgtccggc
      961 ccgaaggagc acgcgcacga cgccttgccc cccggctttc acgcgcagga gcacgagatc
     1021 gccgaggaga tggcgcagcc ggccgccgac caccgcccgc gcgggcatca caagtttcac
     1081 cgcttcgacc gcgcggagga ctgggtcgcg cagttcgacg ccgccgatcg tgctgagtgg
     1141 caaaagccgg acgcggtgat cgccgcgctc gcgctggccc ctgacgcgag cgtcgcggac
     1201 ctcggggccg gcaccggcta cttcgcagtg cgcctcgcgc gggccgtcgc gaagggccgc
     1261 gtgtacgccg aggacctcga gcccgacatg gtccgctacc tcggcgagcg ggccgcgaag
     1321 gagggcctcg gcaacatcac ggccgtgcag ggcgtcgccg acgaccccaa gctgccggcg
     1381 ccggtcgacg tcgcgatcat ggtcgacagt tatcaccaca tcgccgagcc gaccgccttc
     1441 ttcgcccgcg tgcgcgactc gctgcggccg ggcgggctgc tggtgatcgt cgacttcaag
     1501 aaagacgcgc cggacgacgc cccggggcca ccggctgcca tgcgggtcgc cgacgagatc
     1561 gtgacggccc acctgcgcaa gctcggctac gtgcacgagc ggaccgaccg cgacctgctg
     1621 ccgtaccagt acctcgtgtt catgcgtcgc ccgaaatgaa cagcaagggc ccgtggcggc
     1681 gcgatctctt tatataagtg ttgacttata tgatgcccgt ctggcatcac cggggccggg
     1741 ccacagcgcc cgaggagacc cccatgaatt tcgatgttcc tcgccacatc ggcgcggtca
     1801 cccgtgtcgt cgacaatcgc cagcgcgacg gtcggccgac gcgggtcgtc gtcgccacgc
     1861 gcacctatga cagcgacatc gcggacgtct gggacgcgat caccagcgcc gagcggatcc
     1921 cgcggtggtt cttgccggtc tccggcgagc tgcggctcgg cg
//
LOCUS       DASWNJ010000758         3303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_297134, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000758 DASWNJ010000000
VERSION     DASWNJ010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297134"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VGB85_12690"
     CDS             <1..339
                     /locus_tag="VGB85_12690"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEY0134940.1"
                     /translation="HVFIIRDGVLISPPSTEDNLDGITRRSLITLASEDLGLPVLERT
                     IGRTELYVSDEMFLCGTGAEITPVRSVDRRTVGEGAVGPITARLQKHFADVVRGRVEH
                     RLSWLTQVWT"
     gene            359..3079
                     /locus_tag="VGB85_12695"
     CDS             359..3079
                     /locus_tag="VGB85_12695"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY0134941.1"
                     /translation="MRDHFVVRARWYILAVLVALCAWILPGVRDVVEDDDVLAFLPND
                     HADVIAFREIAERFGVLEIGLVGLHKTDGDMLSVERVDEVRALSQALGELPGVRMVLS
                     FTDLPDPQVTEDGLEVAALVPEDLRDPEKIRAKVLGSTDAVGNFISADGKAAALMIFL
                     LHDKDRTGPPRGQILQKVRDVVAERWQGESHVGGAPFVEDAAARSSRSDVNRLSPIVI
                     GVLVLVSAVLLGSITAAGLNLILTGLGVGLVMGAHGRFGIPLTIVSSSLPVMMVALGG
                     AFGVHILAGFQRQQGTSHERASATLRELWLPVVLSGVTTAVAFYALVVMPQVPMKDFG
                     IIAGTGVLALLLLALGVLPALLAVLPGGLLKPRAERPMPMRLRPPLWLMLVLAVAGAA
                     MATRLHADPDMTAVFSEESEPVQSNNFFNKHFGGSNFLQVALEAEHVEVDPNNPDRKP
                     LSEPAVLREIRDIGERVRAIDGVVDLRSVVEPVMLLNEALGGRRGIPETAGRGGRVLT
                     YLQGHPAVAQLITPDAAGALIHIKLAPMSGERQIEVTQQVREILTRNAAGRMRVAKTS
                     DPAVRAVQLAEVGEHLTRLTGKPVDAGTLAVAAPKDLSPAMLAAITEVRDRALDSEDS
                     PVSDVPRVEIDAIDPASLVTPRGAELEALLRDKLPTLAAKDPEGIGFVAKHLGPWTDE
                     VLARFRVEGRCGALGLPLVATEPGGPSCAPVQAALSELDDEEWRVTTGEGRELTLRTR
                     LTGQPVIGAAFAESETRSLFESTLVSLIGLALTLLVARQLRALIPALWTLCVTMGLLA
                     LLGLPISVGTSMISCIAIGAGVDFAIHLGFRARTFSGPDSGQRAVDEIGVVTLISALQ
                     LACAFAVLMASELAPLREFGIGLAIGLVGAALGACWLVPWLYRGAASKQA"
BASE COUNT          432 a         1172 c         1196 g          503 t
ORIGIN      
        1 cacgtgttca tcatccgcga cggcgtgctg atcagcccgc cgagcaccga ggacaacctc
       61 gacggcatca cccgtcgcag cctgatcacg ctggcgagcg aggacctcgg cctgccggtg
      121 ctcgagcgca cgatcggccg caccgagctg tacgtgtccg acgagatgtt cctgtgcggc
      181 accggtgccg agatcacccc ggtccgctcg gtcgaccggc gcacggtcgg cgagggtgcg
      241 gtcgggccga tcaccgcgcg cctgcagaag cacttcgccg acgtcgtccg cggccgcgtc
      301 gagcaccgcc tgtcgtggct gacgcaggtc tggacctgac cgcagggcac ccgccgtcat
      361 gcgtgatcac ttcgtcgtcc gcgcgcgctg gtacatcctc gcggtcctcg tcgccctgtg
      421 cgcgtggatc ttgccggggg tgcgtgacgt cgtggaggac gacgatgtgc tcgcgttctt
      481 gccgaacgac cacgccgacg tgatcgcgtt ccgcgagatc gcggagcgct tcggggtgct
      541 cgagatcggg ctcgtgggcc tgcacaagac cgacggcgac atgctctcgg tcgagcgcgt
      601 cgacgaggtg cgcgcgctgt cgcaggcgct cggcgagctg ccgggcgtgc gcatggtgct
      661 gtcgttcacc gacctgccgg acccgcaggt gacagaggac ggcctcgagg tcgccgcgct
      721 ggtcccggag gacctgcgcg acccggagaa gatccgcgcc aaggtgctcg gatcgaccga
      781 cgcggtcggc aacttcatct cggccgacgg caaggccgcg gcgctgatga tcttcctgct
      841 gcacgacaag gaccgcaccg gtccgccgcg cgggcagatc ctgcaaaagg tccgcgatgt
      901 cgtcgcggag cgctggcagg gcgagagcca cgtcggcggg gcacccttcg tcgaggacgc
      961 ggcggcccgc tcgagccgca gcgacgtcaa ccggctctcg ccgatcgtga tcggcgtgct
     1021 cgtgctggtg tcggcggtgc tgctcggcag catcacggcg gccggcctca acctgatcct
     1081 gaccggcctc ggcgtcggcc tggtgatggg cgcgcacggc cgcttcggca tcccgctgac
     1141 gatcgtcagc agctcgttgc cggtgatgat ggtcgcgctc ggcggcgcct tcggcgtgca
     1201 catcctcgcc ggcttccagc gccagcaggg caccagccac gagcgcgcca gcgcgacgct
     1261 gcgcgagctg tggctgccgg tggtgctcag cggcgtgacg acggcggtcg ccttctacgc
     1321 gctcgtcgtg atgccgcagg tgccgatgaa ggacttcggc atcatcgccg gcaccggcgt
     1381 gctcgcgctg ctgctgctcg cgctcggggt gctgccggcg ctgctcgccg tgctgcccgg
     1441 cggcctgctc aagcccaggg ccgagcgccc gatgccgatg cgcctgcgcc cgccgctgtg
     1501 gctgatgctg gtgctcgcgg tcgccggcgc ggcgatggcg acgcggctgc acgccgaccc
     1561 cgacatgacg gcggtgttct ccgaggagag cgagccggtc cagtcgaaca acttttttaa
     1621 taagcacttc ggcggcagca acttccttca ggtcgcgctc gaggcggagc acgtcgaggt
     1681 cgacccgaac aaccccgacc gcaagccgct gtcggagccg gcggtgctgc gcgagatccg
     1741 tgacatcggc gagcgcgtgc gggcgatcga cggcgtcgtc gacctgcgat ccgtcgtcga
     1801 gccggtgatg ctgctcaacg aggcgctcgg cgggcgccgt gggatccccg agaccgccgg
     1861 ccgtggcggc cgcgtgctga cgtacttgca gggccacccc gcggtcgccc agctgatcac
     1921 ccccgacgcc gccggcgcgc tgatccacat caagctcgcg ccgatgtccg gcgagcggca
     1981 gatcgaggtc acgcagcagg tccgcgagat cttgacgcgc aacgcggcgg gccgcatgcg
     2041 cgtggcgaag acctccgatc cggccgtgcg cgcggtgcag ctggccgagg tcggcgagca
     2101 cctgacgcgc ctgaccggca agcccgtcga cgccgggacg ctcgccgtcg ccgcgccgaa
     2161 ggatctgtcg ccggcgatgc tggccgcgat caccgaggtg cgcgaccgcg cgctcgactc
     2221 cgaggacagc ccggtcagcg acgtgcctcg cgtcgagatc gacgcgatcg accccgccag
     2281 cctggtcaca ccgcgcggcg cggagctcga ggcgctgctg cgcgacaagt tgccgaccct
     2341 cgccgccaag gaccccgagg gcatcggctt cgtcgccaag cacctcggcc catggaccga
     2401 cgaggtgctc gcgcgcttcc gcgtcgaggg ccgctgcggc gcgctcgggc tgccgctggt
     2461 tgccacggag ccgggcggcc cgagctgcgc gccggtccag gccgcgctgt cggagctcga
     2521 cgatgaagag tggcgcgtca ccaccggcga gggccgcgag ctgacgctgc gcacgcgcct
     2581 caccggccag ccggtgatcg gggccgcgtt cgcggagtcg gagacgcgca gcctgttcga
     2641 gtcgacgctc gtctcgctga tcggcctcgc gctgacgctg ttggtcgcac ggcagctgcg
     2701 cgcgctgatc cccgcgctgt ggaccctgtg cgtcacgatg ggcttgctcg cgctgctggg
     2761 tctccccatc agcgtcggga cgagcatgat ctcgtgcatc gcgatcggcg caggcgtcga
     2821 cttcgcgatc cacctcgggt ttcgtgcgcg gacgttcagc ggtcccgaca gcggtcagag
     2881 ggccgtggac gagatcggtg tcgtcaccct gatctcggcg ctgcagctcg cctgcgcctt
     2941 cgcggtgctg atggcctccg agctcgcgcc gctgcgagag ttcgggatcg gcctggcgat
     3001 cgggctcgtc ggcgcggccc tcggcgcctg ctggctggtc ccgtggctgt atcgcggcgc
     3061 tgcgtccaag caggcgtgaa gctcgttgcg caccggatcg ccgccgacgc cgcatcccga
     3121 agtggcacga tcaccgcgcg gccgggcgtg tgatgcctgt caccaaacgc cgcaaaaccc
     3181 cctccagggg ccgttgtgcg gctgtttcgc gggtggcgcc ggagcgtaca agaagtttgg
     3241 ggctcgcaga atagtcgggg gcgagcggcg tccaaggact gggcgcgtac ctgcgcccgc
     3301 gct
//
LOCUS       DASWNJ010000759         4499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_366617, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000759 DASWNJ010000000
VERSION     DASWNJ010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366617"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VGB85_12700"
     CDS             <1..186
                     /locus_tag="VGB85_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134942.1"
                     /translation="PEHHYVTWSRRQGDHMRDSIARHRDLAPAAAADLHRLLNQSVLL
                     VPIPCALPEAEANDGQP"
     gene            191..1099
                     /locus_tag="VGB85_12705"
     CDS             191..1099
                     /locus_tag="VGB85_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0134943.1"
                     /translation="MTDPPEPSSRPFAIACGAIALPLLAALTWSVLDDPGGPVTTAKI
                     ASAPAPTSAPTPSAPAIRPQPPAVAGSLTVDPPRKLTRLGQLGLGALHGDGRLVPSSL
                     GPALGRRVSVVNVWATYCAPCMRELPRLLDLFAANAWGNDLRFVPVLLEAPDRQNAAQ
                     QAALTSLRQAPAVRQVLVDLTPAGALQTLLHDADLLPERATLPLTLVLDCEQRLRWLH
                     RGEITDTAALAAVLEQLRAELPRCAPPPQEVVAADGCGDDYCDPERAEDCATCQRDCA
                     CPENRECVPITGRRARCNFRTQGLKD"
     gene            1124..2593
                     /locus_tag="VGB85_12710"
     CDS             1124..2593
                     /locus_tag="VGB85_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134944.1"
                     /translation="MLYALAPLLLSAGLVAPSPDPPLLPASATSTHAVASPASSTPDA
                     VRTATTPAEESARSATARAHYAARRYREAALVYERLWHDTQAPKYRFNAGMARAAAEH
                     DAAAIVHWQAYLTLAPTIAAGERSMLEAEIAAAKRRTTPVQLRVQGPLAPATLTLRVP
                     DGAPQGPRDPIALVPAATIDLSLEPGTWIATLTRPGRPEATASFEVTAGAEPTITLGA
                     DAPAPTPAPTPEVIPPPPAPATLTLDLGPRRALARGITVTPIGPAPADARTLHTTSAT
                     WTLPAGAWTLRATAPDHRPTTTTITLGPADARRLALHLPRDRPANTRLGLGLGLGGAG
                     LVLLTTGTVLTARTSGALTCFDRESCTAAADRVLDRSIGASLMGAGLGVAAPALTAGL
                     GRRASTDRALMIEAGLGAVILAGGVGWYFSEVTRTTALDHMPRQHAAAALRGLGGGML
                     GAAVVGLIVRRLTRSRPSTVSLAPSFSRHTRGLALQAHF"
     gene            2606..3838
                     /locus_tag="VGB85_12715"
     CDS             2606..3838
                     /locus_tag="VGB85_12715"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0134945.1"
                     /translation="MSNAARLLPCALLTLAAACKPDCPQGATDRHGYNCTPVPGDTDD
                     ASTSTGDDPSTTSDDSSSTGNNTSTTIPHPCDDDPACGPDGDANTCPEQCSVCGDGLV
                     SGDEACDRGPNNQSYWLTTPPPDACSDTCTTLVQWCGDAILNGDEPCDNGTNADSDYS
                     LTTPPADACAPGCIIPGHCGDAILNGPELCDDAAQTPTCELSCLPPTCGDGAHNPLAG
                     EACDDANTLDGDGCSADCHSIERLVFVSSAQYEGDFVPMTNNPDALEGLALADARCQQ
                     LATAASLPGAYKAWLSTTTDSPSTRFDTRFTGLYRLTSDGAPIIAIGWQGLTSGALLH
                     PIDTDESGTTTQKNVWTNTLPDGTKASDGDCSGWTLNQLGMSTSVGYTLATDSTWTNA
                     ASGQLCSSTFRLYCFQDV"
     gene            3844..>4499
                     /locus_tag="VGB85_12720"
     CDS             3844..>4499
                     /locus_tag="VGB85_12720"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0134946.1"
                     /translation="MRSSSFLALILLPACLQSSTDLSDPKGTGTDPAGASSGGNEPIG
                     TTSDGAGTTTPDPCDDDPACGPDEANTCPAQCSICGDGLVSGDEACDHGPDNQTYWLT
                     TPPPDACSDTCTTQVQWCGDATVDASELCDNGTNLDPAYSIAPPPATACAPGCVLPGH
                     CGDAILNGPELCDDAAQTPTCELSCLPPTCGDGAHNPLAGEACDDANTLDGDGCSADC
                     "
BASE COUNT          654 a         2047 c         1256 g          542 t
ORIGIN      
        1 cccgagcacc actacgtcac ctggtcgagg cgccagggtg atcacatgcg cgactcgatc
       61 gcccgccacc gcgacctcgc gcccgccgcc gcggccgacc tccaccgcct gctcaatcaa
      121 tccgtgctgc tggtcccgat cccctgcgcg ctgcccgagg cggaggccaa tgatggccag
      181 ccctgagccc gtgaccgacc cgccggagcc ctcgtcgcgg cctttcgcga tcgcctgcgg
      241 cgcgatcgcc ttgccgctgc tcgccgcgct cacatggagc gtgctcgacg atcccggcgg
      301 accggtcacg accgcgaaga tcgccagcgc ccccgcgccg acctccgccc cgacgccgag
      361 cgcccccgcg atccgcccgc agccgcccgc ggtcgccggg tcgctgaccg tcgatccgcc
      421 gcgcaagctg acccgcctcg gccagctcgg cctcggtgcg ctgcacggcg acgggcgcct
      481 ggtcccgagc tcgctgggcc ccgcgctcgg ccggcgggtc agcgtcgtca acgtgtgggc
      541 cacctactgc gccccgtgca tgcgcgagct gccgcgcctg ctcgacctgt tcgccgcgaa
      601 cgcgtggggc aacgacctgc gcttcgtgcc cgtgttgctc gaggcccccg accgccagaa
      661 cgccgcgcaa caggccgcgc tgaccagctt gcggcaggcc ccggcggtcc ggcaggtgct
      721 cgtcgacctg acccccgccg gcgcgctcca gaccctgctg cacgacgccg acctcttgcc
      781 cgagcgcgcg accctcccgc tgaccctcgt cctcgactgc gagcagcgcc tgcgctggct
      841 gcatcgcggc gagatcaccg acaccgccgc gctcgcggcc gtgctcgagc agctgcgcgc
      901 cgagctgccg cgctgcgccc cgccgccgca ggaggtggtc gccgcggacg gctgcggcga
      961 cgactactgc gaccccgagc gcgccgagga ctgcgcgacc tgccagcgcg actgcgcctg
     1021 ccccgagaac cgcgagtgcg tgccgatcac gggccgccgc gcgcgctgta acttccgcac
     1081 gcagggcctc aaagactgac gccgaccgca ccccaggctc cccatgctct acgccctcgc
     1141 cccgctcctc ctctcggccg gcctcgtcgc cccgagcccc gacccgcccc tgctcccggc
     1201 gagcgccacg agcacccacg ccgtcgcgtc gccggcatcc tcgacgcccg acgccgtccg
     1261 caccgcgacc acccccgccg aggagtcggc ccgcagcgcg accgcgcgag cgcactacgc
     1321 cgcgcgacgc taccgcgagg ccgcgctggt ctacgagcgc ctgtggcacg acacccaggc
     1381 gcccaagtac cggttcaacg ccggcatggc gcgcgccgcc gcggagcacg acgccgccgc
     1441 gatcgtccac tggcaggctt acctgacgct cgcgccgacg atcgccgccg gcgagcgcag
     1501 catgctcgag gccgagatcg cggccgcgaa gcgccgcacc acgccggtcc agctgagggt
     1561 ccagggcccg ctcgcccccg cgaccctcac cctgcgcgtc cccgacggcg ccccccaggg
     1621 cccacgcgac ccgatcgccc tcgtccccgc cgccacgatt gacctgtccc tcgagccagg
     1681 tacgtggatc gccacgctca cgcgacccgg gcgacccgag gccaccgcca gcttcgaggt
     1741 caccgccggc gccgagccga cgatcactct cggcgccgac gcgcccgccc cgaccccggc
     1801 ccccacgccc gaagtcatcc ctccaccgcc cgcgcccgcg accctcaccc tcgacctcgg
     1861 cccacgccgc gccctcgccc gcggcatcac ggtcacgccc atcggccccg ccccggccga
     1921 cgcccgcacc ctgcacacca ccagcgcgac ctggaccttg cccgccggcg cctggaccct
     1981 gcgcgccacg gccccggacc accgcccgac caccaccacc atcaccctcg gccccgccga
     2041 cgcccgccgc ctcgccctgc acctcccacg cgaccgcccc gccaacaccc gcctcggcct
     2101 cggcctcggc ctcggcggcg ccggcctcgt cctcctgacc accggcaccg tcctgaccgc
     2161 ccgcaccagc ggcgcgctga cctgcttcga ccgcgagtcc tgcaccgccg ccgccgaccg
     2221 tgtgctcgac cgatcgatcg gcgcatccct gatgggcgcc ggcctcggcg tcgccgcccc
     2281 ggccctgacc gcaggcctcg gccgcagggc ctccaccgac cgcgcgctga tgatcgaggc
     2341 cggcctcggc gcggtgatcc tcgccggcgg cgtcggctgg tacttctcgg aggtcacgcg
     2401 aaccaccgcc ctcgatcaca tgccccggca gcacgccgcg gccgccctgc gcggcctcgg
     2461 cggtggcatg ctcggcgccg cggtcgtcgg cctgatcgtc cgccgcctga cccgcagccg
     2521 cccctccacg gtctcgctcg ccccgagctt ctctcgccac acccgcggcc tcgcgctcca
     2581 ggcccacttc tagcccgaag atcccatgtc gaacgccgcc cgcctcctcc cctgtgcgct
     2641 gctgaccctc gccgccgcct gcaagcccga ctgcccgcag ggtgccaccg accgccacgg
     2701 ctacaactgc acgccggtcc ccggcgatac cgacgacgcg agcacgagca ccggcgacga
     2761 ccccagcacc accagcgacg acagcagcag cactggcaac aacaccagca ccaccatccc
     2821 gcacccctgc gacgacgacc ccgcctgcgg ccccgacggg gacgccaaca cctgccccga
     2881 gcaatgcagc gtctgcggcg acggcctcgt ctccggcgac gaggcctgcg accgtggtcc
     2941 caacaaccaa agctactggc tcaccacccc gccccccgac gcctgcagcg acacctgcac
     3001 caccctcgtc cagtggtgcg gcgacgccat cctcaacggc gacgagccct gcgacaacgg
     3061 caccaacgcc gactccgact acagcctcac cacgcccccc gccgacgcct gtgcccccgg
     3121 ctgcatcatc cccggccact gcggcgacgc catcctcaac ggccccgaac tgtgcgacga
     3181 cgccgcccag acccccacct gcgagctctc ctgcctccct cccacctgcg gcgacggcgc
     3241 ccacaaccca ctcgccggcg aagcctgcga cgacgccaac accctcgacg gcgacggctg
     3301 ctccgcagac tgccactcca tcgagcgcct cgtcttcgtc tccagcgccc agtacgaggg
     3361 tgacttcgtc ccgatgacca acaaccccga cgccctcgaa ggcctcgccc tcgccgacgc
     3421 tcgctgccaa caactcgcca ccgcggcttc ccttcccggc gcctacaagg cctggctcag
     3481 caccaccacc gattccccct ccacccgctt cgacacccgc ttcaccggcc tctaccgcct
     3541 cacctccgac ggtgccccca tcatcgccat cggctggcaa ggcctcacct ccggcgctct
     3601 cctccatccc atcgacacgg atgagtccgg taccactact cagaagaatg tctggacaaa
     3661 caccctccct gacggcacca aggcttccga cggcgactgc tccggctgga ctcttaatca
     3721 gcttggtatg agcacttcag ttggttacac ccttgcaacc gatagcacct ggaccaacgc
     3781 cgccagcggc caactttgct cgagcacatt tcgtctttac tgcttccagg atgtttgact
     3841 ttcatgcgct cgtcttcctt cctcgcgctc atcctgctcc ccgcttgcct tcagagcagc
     3901 accgacctct ccgatcccaa aggaacgggc acggaccctg ccggcgcaag cagtggcggc
     3961 aacgaaccca tcggcaccac aagcgacggt gccggtacga ccacaccgga cccctgcgac
     4021 gacgaccccg cctgcggccc cgacgaggcc aacacctgcc ctgcgcagtg cagcatctgc
     4081 ggcgacggcc tcgtctccgg cgacgaggcc tgcgaccacg gccccgacaa ccagacctac
     4141 tggctcacca ccccgccccc cgacgcctgc agcgacacct gcaccaccca agtccagtgg
     4201 tgcggcgacg ccactgtcga cgccagcgag ctctgcgaca acggcaccaa cctcgacccc
     4261 gcctacagca ttgccccgcc ccccgccacc gcctgcgccc ccggctgcgt ccttcccggc
     4321 cactgcggcg acgccatcct caacggcccc gaactgtgcg acgacgccgc ccagaccccc
     4381 acctgcgagc tctcctgcct ccctcccacc tgtggcgacg gcgcccacaa cccactcgcc
     4441 ggcgaagcct gcgacgacgc caacaccctc gacggcgacg gctgttccgc agactgcaa
//
LOCUS       DASWNJ010000760         3111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_650149, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000760 DASWNJ010000000
VERSION     DASWNJ010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650149"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2101
                     /locus_tag="VGB85_12725"
     CDS             <1..2101
                     /locus_tag="VGB85_12725"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEY0134947.1"
                     /translation="GDPATGDDTGAASTSDAGTTDASTDTTTGEPSACAEAEAKLGAR
                     VCAHEVADDATWSALSLGVDRIDQVRTGKYLAPARDDARLPALVLDVNTYELHYQFLR
                     AAFPDRFPDLTPESYERMITDPAERELFAGSITEYRTATGERLFGYVAWDPQTDLATT
                     VTCSQFQALHASLAQVVTVGPLAAVPTGELQRQVLADCDVPSYDATAAVDYEPYTTAV
                     GFGTVRRYTPAELDAATQAAAYGFQDILVLQEAPLDIERVISGAVTGGRQAELSHLNV
                     RSAARGTPNCYLRDAHARMAPWDGELVRLECGKTGLEVRAASPEEAQSWWDQLRPDPV
                     EIPAPDLDWPEITPLLELPTATPDERLLGVRRYGAKGRNLATLYQRIDAALQIPGFLI
                     PFHYYDAYLASQQWTVDLGAGPETLSLAAAIDRLLMDPQFRTDPALRRQRLDALYEAI
                     RDGACDPALLADLHTRLLETFGADTVMVRFRSSSNAEDAPGFSGAGLYESTSACLADD
                     LDADKLGPSRCDPSEPDERGVCRAVKKVWASLWLVRAFEEREWYGIDHHEAAMGILVD
                     QRFGNEKANMVVFTGDPNLAGDQRILVNAQLGDLEVVSPEPGTWPEQTRLTMVDGAVT
                     KIDRVRGSSELPEGQHVLSDAQLEQLGAHMWQIREVFPIDEPPPPDTAVLLDSEWKVR
                     PDDTLVIKQVRPYLRRE"
     gene            complement(2109..>3111)
                     /locus_tag="VGB85_12730"
     CDS             complement(2109..>3111)
                     /locus_tag="VGB85_12730"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF009005.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEY0134948.1"
                     /translation="AGHDIERLGGRVTFEDVAALLWTGALPDAEGRAAQQRALAEGRV
                     AAFERLAATGDATALPDGMDALRATTAHLSAQAGFDAPARLTGALAVYAAAWGRARRG
                     ARPIAPDPTLSHAADYLRMLGGEPQDAARVAGLDAYLVTVADHGLNASTFAARVVTST
                     GSDGVSAIVAAIGTLKGPLHGGAPGPVLDMLDAVGEPDNASAWIDAELAAGRRIMGMG
                     HRIYRVRDPRAAVLERAIAQLEQAGISTGRLALARAVERAAEGALRLKYPGRPLAANV
                     EFYTAVLLDTVGIDRVAFAPTFAVGRVAGWYAHVLEQRATGRLIRPASRYVGPAPAP"
BASE COUNT          443 a         1170 c         1114 g          384 t
ORIGIN      
        1 gggtgatcct gccaccggcg acgacaccgg cgcggcgagc acgagcgacg ccgggaccac
       61 cgatgcgtcg accgacacga ccaccggaga gccgtcggcc tgcgccgagg ccgaggcgaa
      121 gctcggcgcc cgcgtgtgcg cgcacgaggt cgccgacgac gcgacctggt cggccctgtc
      181 gctgggagtg gaccggatcg atcaggtgcg caccggcaag tacctcgcgc cggcccgcga
      241 cgacgcccgg ctgcccgcgc tcgtgctcga cgtcaacacc tacgagctgc attatcagtt
      301 cctgcgcgcg gccttccccg atcgcttccc cgatctgacg cccgagtcct acgagcgcat
      361 gatcaccgac cccgcggagc gcgagctgtt cgccgggtcg atcaccgaat atcgcaccgc
      421 caccggcgag cgcctgttcg gctacgtcgc ctgggacccg cagaccgacc tcgccaccac
      481 ggtgacgtgc agccagttcc aggcgttgca cgcatcgctc gcgcaggtcg tcaccgtcgg
      541 cccgctcgcc gcggtgccga ccggcgagct gcagcgccag gtcctcgccg actgcgacgt
      601 gccgagctac gacgcgaccg ccgcggtcga ctacgagccc tacaccacgg cggtcggctt
      661 cggcaccgtg cgccgctaca cgcccgccga gctcgacgcc gccacgcagg cggccgcgta
      721 tggctttcag gacatcctcg tcttgcagga ggccccgctc gacatcgagc gcgtgatctc
      781 cggggcggtg accggcgggc ggcaggccga gctgtcgcac ctcaacgtcc gcagcgccgc
      841 gcgcgggacc ccgaactgct acctgcgcga cgcccacgcg cgcatggccc cgtgggacgg
      901 cgagctcgtg cgcctcgagt gcggcaagac cggcctcgag gtccgcgcgg ccagcccgga
      961 ggaggcgcag agctggtggg accagctgcg ccccgacccg gtcgagatcc ccgcgcccga
     1021 cctcgactgg ccggagatca cgccgctgct cgagctgccg accgcgaccc ccgacgagcg
     1081 cctgctcggc gtgcgccgct acggcgccaa gggccgcaac ctcgccacgc tgtatcagcg
     1141 catcgacgcc gcgttgcaga tccccgggtt cttgatcccg tttcattatt acgacgccta
     1201 cctcgcgtcg cagcagtgga ccgtcgatct cggcgccggc ccggagaccc tgagcctcgc
     1261 cgcggcgatc gatcggctgt tgatggaccc gcagtttcgc accgaccccg cgctgcggcg
     1321 ccagcggctc gacgcgctct acgaggcgat ccgcgacggc gcgtgcgatc ccgcgttgct
     1381 cgcggacctg cacacgcggc tgctcgagac cttcggggcc gacaccgtca tggtccgctt
     1441 tcgcagcagc agcaacgccg aggacgcgcc cggcttcagc ggcgcgggcc tctacgagtc
     1501 gacgagcgcg tgcctggccg atgacctcga cgccgacaag ctcggcccga gtcgctgcga
     1561 tcccagcgag cccgacgagc gcggcgtgtg ccgggccgtc aagaaggtgt gggcctcgct
     1621 gtggctggtg cgcgccttcg aggagcgcga gtggtacggc atcgaccacc acgaggccgc
     1681 gatggggatc ctcgtcgacc agcgttttgg caacgagaag gccaacatgg tcgtcttcac
     1741 cggcgatccg aacctcgccg gcgaccagcg gatcctggtc aacgcgcagc tcggcgacct
     1801 cgaggtcgtc agccccgagc ccggcacctg gccggagcag acgcggctga cgatggtcga
     1861 cggcgcggtg acgaagatcg accgcgtgcg tgggtcgtcc gagctgcccg agggtcagca
     1921 cgtactgagc gacgcgcaat tggagcagct cggcgcgcac atgtggcaga tccgcgaggt
     1981 gttcccgatc gacgagccgc cgccgccgga caccgccgtg ctgctcgaca gcgagtggaa
     2041 ggtccggccg gacgatacgc tggtcatcaa gcaggtccgg ccctacctgc gccgcgagtg
     2101 aggccgcctc acggggccgg cgcaggcccg acgtagcgcg aggccgggcg gatcagccgc
     2161 ccggtcgcgc gctgctcgag cacgtgggcg taccagcccg cgacgcgacc gacggcgaag
     2221 gtcggcgcga aggcgacgcg gtcgatgccg acggtgtcga gcagcaccgc ggtatagaac
     2281 tcgacgttgg cggcgagcgg gcggcccggg tacttgagcc gcagggcgcc ctcggccgcg
     2341 cgctcgacgg cgcgggcgag cgcgagacgg ccggtcgaga tgccggcctg ctcgagctgc
     2401 gcgatcgccc gctcgagcac cgcggcgcgc gggtcacgga cgcgatagat gcgatgaccc
     2461 atgcccatga tccggcggcc ggcggccagc tcggcgtcga tccaggcgct ggcgttgtcg
     2521 ggctcgccga ccgcgtcgag catgtcgagc accggccccg gggcgccgcc gtgcagggga
     2581 cccttgagcg tgccgatcgc cgcgacgatc gccgagaccc cgtcggagcc ggtcgaggtg
     2641 acgacccgcg ccgcgaaggt cgaggcgttg aggccatgat ccgcgaccgt cacgagatag
     2701 gcgtcgagcc ccgcgacgcg ggcggcgtcc tgcggctcgc cgccgagcat gcgcagatag
     2761 tcggccgcgt gcgagagggt cgggtccggc gcgatcggcc gcgcaccccg acgcgcgcgg
     2821 ccccacgcgg cggcataaac cgcgagcgcg ccggtcagcc tggccggcgc gtcgaagccg
     2881 gcctgcgccg acaggtgcgc cgtggtggcc cgtagcgcgt ccataccgtc ggggagggca
     2941 gtcgcgtcgc cggtcgccgc gagccgctcg aaggccgcca cgcgcccctc ggcgagcgcc
     3001 cgctgctgcg ccgcgcgccc ctccgcgtcg ggcagggcgc cggtccacag cagcgcggcg
     3061 acatcctcga aggtcacccg cccgccgagc cgctcgatgt cgtgccccgc g
//
LOCUS       DASWNJ010000761         3232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_262104, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000761 DASWNJ010000000
VERSION     DASWNJ010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262104"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VGB85_12735"
     CDS             complement(<1..842)
                     /locus_tag="VGB85_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134949.1"
                     /translation="MRVSPLWTLWPALLLALAFVLSPRTGAANSRDLKWKTLQTEHFW
                     IHYYAGSEHAAERAAMVLERAHARLSVGLGHSPRIRTHVVMNDATDAANGSATVFSFP
                     RIDANITAPDSLSVLESYDDWLDALLTHEYTHIVHNDTIHGLPRVINSILGFGVLGRV
                     WPPNGLQPRWMIEGLATYEESRLTSQGRHRSTQFDMMLRMAILEQGFPTLDRVSAPAT
                     IFPHTTSVYLYGLHLVHYMGTRYGHDKLREFSHRYGGRTIPYSINRTVQEVYGVDFEQ
                     LWKE"
     gene            complement(994..1917)
                     /locus_tag="VGB85_12740"
     CDS             complement(994..1917)
                     /locus_tag="VGB85_12740"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0134950.1"
                     /translation="MRDPGFTLSTPEASRLVSLSFGRVFLRDHGRHQDPTRPPVLLLH
                     GMLVTSHSFTRLIPALAGDRRVLAPDLPGCGDSDRPPPVLCADYSPRWQAAALLELLD
                     HLGLARIDLVGHSWGGAIAVCLIDAAPERVRRLVLVDPTLMPMVIPIEGRLAQVPRLG
                     PYVFKNMYRRNELRRYLGRAFSSEHLVSEADVDLYWDRLARAGGREAAHAMLMQLMQP
                     DTMAAMLPRLRAIAAPTVVVFGERDAIVPHDHAERITAAIPGATLRWLPGCGHTPAEE
                     QPEALAAVLHEHLDAAQLGPAAPGPSPRLEA"
     gene            complement(1914..>3232)
                     /gene="ptsP"
                     /locus_tag="VGB85_12745"
     CDS             complement(1914..>3232)
                     /gene="ptsP"
                     /locus_tag="VGB85_12745"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEY0134951.1"
                     /translation="GAVVVAHDLSPADTARLHRCHVVGIITGVGGRTSHSAIMARSLQ
                     IPAVVGIEAILEIVDSGDLVVVDGVHGHVIVRPEPEEVTHHAGQRERYNAFEQRVRKE
                     HAFPAISSDGLHVTVRANLAISEELDLALRYGAEGVGLYRSEYMFMNRDVLPNEEEHY
                     RMAKLVLRRCAPYPVVFRTFDLGSDKSSELLDFDEENNPAMGMRSLRLALRERPMFLE
                     QIRGFLRAGVHGPLAIMLPLVGSIEELEQGLEAIEEAREQLASAGMAHAEDLAIGIMV
                     ETPAAAMIADVLAQRVDFISIGTNDLIQYTLAIDRENEAVAYLYQPLHPAILRLIKIV
                     AEAGQRNKVPVSLCGEMAADPRYTWVLIGLGITELSMHPSAIPVIKNIIRSSTSTEMV
                     ALAERALLSQSGREAEELVFTAMHARFPEHLLHGGGQGTGGESTTP"
BASE COUNT          461 a         1086 c         1210 g          475 t
ORIGIN      
        1 aactccttcc acagctgctc gaagtcgacg ccgtacacct cctgcaccgt gcggttgatg
       61 ctgtacggga tcgtgcggcc gccgtagcgg tggctaaact cgcgcaattt gtcgtgcccg
      121 tagcgcgtgc ccatgtagtg cacgaggtgc aggccgtaca ggtacacgga ggtcgtgtgc
      181 gggaagatcg tcgccggcgc cgacacgcga tcgagggtcg ggaagccctg ctcgaggatc
      241 gccatgcgca gcatcatgtc gaactgcgtc gaccggtggc ggccctgcga ggtcaggcgc
      301 gactcctcgt aggtggcgag gccctcgatc atccagcgcg gctggaggcc gttgggcggc
      361 cacacgcggc cgagcacgcc gaagccgagg atcgagttga tgacccgcgg cagcccgtgg
      421 atcgtgtcgt tgtggacgat gtgggtgtac tcgtgggtca gcagcgcgtc gagccaatcg
      481 tcgtagctct cgagcacgga cagcgagtcg ggcgcggtga tgttggcgtc gatgcgcggg
      541 aagctgaaca ccgtcgcgct gccgttggcg gcgtcggtgg cgtcgttcat caccacgtgg
      601 gtgcggatcc gcgggctatg gccgagcccg acgctcaggc gcgcgtgggc ccgctcgagc
      661 accatcgcgg cccgctcggc ggcgtgctcg ctgcccgcgt agtagtggat ccagaagtgc
      721 tcggtctgca gcgtcttcca cttgaggtcg cgcgagttgg ccgcgcctgt cctcggggac
      781 aggacgaagg ccagcgccag cagcagcgcc ggccacagcg tccacagggg ggagacccgc
      841 accgggcgcc agcgtagcag gccggggcgg cgcgctccag cgccctgccc cgccccggcg
      901 cgcgctccgg cggccggcga cgcttgtacg cgcaaatgcc cgatgcgatc gcggcggtcg
      961 ccgtgctcgc ggtgacctgg ctcttggtac aggtcaggct tcgagtcgcg gcgagggccc
     1021 gggcgccgcc gggccgagct gcgcggcgtc gaggtgctcg tgcagcaccg cggcgagcgc
     1081 ctcgggctgc tcctcggcgg gcgtatggcc gcatccgggc agccagcgca gcgtcgcgcc
     1141 ggggatcgcg gcggtgatcc gctcggcgtg gtcgtgcggc acgatcgcgt cgcgctcgcc
     1201 gaacacgacg acggtcgggg ccgcgatcgc gcgcaggcgc ggcagcatcg ccgccatcgt
     1261 gtcgggttgc atcagctgca tcagcatcgc gtgggccgcc tcgcggccgc cggcgcgggc
     1321 gaggcggtcc cagtacaggt cgacgtcggc ctcgctgacc aagtgctcgc tcgagaaggc
     1381 gcgcccgagg tagcggcgca gctcgttgcg cctgtacatg tttttgaaga catagggccc
     1441 gaggcgcggg acctgggcga gccggccctc gatcgggatc accatcggca tcagcgtcgg
     1501 gtcgacgagc acgagccggc ggacgcgctc gggcgcggcg tcgatcaggc acacggcgat
     1561 cgcaccaccc cacgagtgac cgacgaggtc gatccgcgcg agaccgaggt ggtcgagcag
     1621 ctcgagcagc gccgccgcct gccagcgcgg gctgtagtcc gcgcacagca ccggcggcgg
     1681 ccgatcgctg tcgccgcagc cgggcaggtc cggcgcgagc acgcggcggt cgccggcgag
     1741 cgcggggatc aggcgcgtga agctgtggct ggtcaccagc atgccgtgca gcagcaacac
     1801 cggcggccgc gtcgggtcct gatggcgacc gtgatcgcgg aggaacacgc ggccgaacga
     1861 cagcgagacc aggcgtgagg cctcgggcgt gctcagggtg aagccggggt ccctcacggc
     1921 gtcgtcgact ctccgccggt cccctgcccg ccgccgtgca gcaggtgctc ggggaagcgg
     1981 gcgtgcatcg cggtgaacac caattcttcg gcctcacggc ccgactgcga cagcagggcg
     2041 cgctcggcca gcgcgaccat ctccgtcgag gtcgagctgc ggatgatgtt cttgatcacc
     2101 gggatcgcgg acgggtgcat cgacagctcg gtgatgccga ggccgatcag cacccaggtg
     2161 tagcgcgggt ccgcggccat ctcgccgcac aggctgaccg ggaccttgtt gcgctggccg
     2221 gcctcggcga cgatcttgat caggcgcagg atcgccgggt gcagcggctg gtacaggtag
     2281 gcgaccgcct cgttctcgcg gtcgatcgcc agggtgtact ggatgaggtc gttggtgccg
     2341 atgctgataa aatcgacgcg ctgcgcgagc acgtcggcga tcatcgcggc ggccggggtc
     2401 tcgaccatga tgccgatcgc caggtcctcg gcgtgggcca tgccggcgct ggcgagctgc
     2461 tcgcgcgcct cctcgatcgc ctcgaggccc tgctcgagct cctcgatcga tccgaccagc
     2521 ggcagcatga tcgccagcgg accgtgcacg ccggcgcgca aaaacccgcg gatctgctcg
     2581 aggaacatcg gccgctcgcg cagcgccaga cgcagcgagc gcatgcccat cgccgggttg
     2641 ttctcctcgt cgaagtcgag cagctcgctc gacttgtcgg acccgaggtc gaaggtgcga
     2701 aacaccaccg ggtacggcgc gcagcggcgc agcaccagct tggccatgcg gtagtgctcc
     2761 tcctcgttgg gcagcacgtc gcggttcatg aacatgtact cgctgcggta caggccgacg
     2821 ccctcggccc cgtagcgcag cgcgaggtcg agctcctcgc tgatcgcgag gttggcgcgc
     2881 acggtgacat gcaggccgtc gctcgagatc gccgggaacg cgtgctcctt gcgcacgcgc
     2941 tgctcgaacg cgttgtagcg ctcgcgctgg ccggcgtggt gcgtgacctc ctcgggctcg
     3001 gggcggacga tcacgtggcc gtgcacgccg tcgacgacga cgaggtcgcc cgagtcgacg
     3061 atctcgagga tcgcctcgat gccgacgacc gcggggatct gcagcgagcg cgccatgatc
     3121 gcgctgtgcg aggtgcggcc gccgacgccg gtgatgatgc cgacgacgtg gcagcggtgc
     3181 aggcgagcgg tgtccgcggg gctgaggtcg tgggcgacga cgacggcgcc gg
//
LOCUS       DASWNJ010000762         4096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_155495, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000762 DASWNJ010000000
VERSION     DASWNJ010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155495"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VGB85_12750"
     CDS             complement(<1..510)
                     /locus_tag="VGB85_12750"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0134952.1"
                     /translation="MEQLARIGRFVVLRKLGEGGMGVVYAAYDEELERKLAIKLVLPG
                     LRSERASIGETRLLREAQALARVSHPNVVQVYEVGSVGEAVYIAMELVRGHTLGEWLA
                     AKPRRWRDVLGVFQQAGAGLAAAHAAGVVHRDVKPDNILVGDDGRVRVLDFSLARPVE
                     GQPAPLTADP"
     gene            598..1104
                     /locus_tag="VGB85_12755"
     CDS             598..1104
                     /locus_tag="VGB85_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HEY0134953.1"
                     /translation="MTTLPLLACVDVDYRGAGAVAAAILFLDWSTPHALAEHVVAVDR
                     VAPYRPGAFYERELPCIMAVLARSTWPIATIVVDGHVWLGPEEPGLGAHLYLALGGHI
                     PVVGVAKSPYRGAPAAELLRGDSQRPLYVTAAGVDPEIAADNIRRMHGPHRLPTLLKA
                     VDSLCRRG"
     gene            complement(1105..1581)
                     /locus_tag="VGB85_12760"
     CDS             complement(1105..1581)
                     /locus_tag="VGB85_12760"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY0134954.1"
                     /translation="MPVIRLTTEIDAPVDRCFDLARSVDLHMHSTAATGETIVGGVRS
                     GLMGPGDEVTWRARHLGVWQRLTSTISAYDRPHHFRDSMVRGAFARFDHDHYFEELAG
                     RTVMRDVFDFSSPLGPLGRLADAVFLTAYLRRFLERRNHVLQTVAESDEWRALIRA"
     gene            complement(1581..3365)
                     /locus_tag="VGB85_12765"
     CDS             complement(1581..3365)
                     /locus_tag="VGB85_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134955.1"
                     /translation="MPVPWFALVLALAAPAAAAASAPTSKLELAVTAADTTLRAAKLG
                     PRTFDYNGAIARTAGLDPDLLAAQLDALTSACPGPGPCAPLQPHTDLVDRLARALGRL
                     GGDRHAGSLLRASRRGSFAASQALDDLRTRAMVEALPRARCDPPSPAELGRAAAGLAG
                     FLVVRVRAGVLTAEPLQPRERDDLAYFLAALGDAGREVGAPAEASTSIPGQPGKPGAQ
                     DQLRTDALAQLQLATHAGDLAGAIAAGRRYLASFGYPGALDGAIDPDQAWGGARHSFV
                     MRDLAELAELTGELTLAYDLNRRADPGGGMCGTSYWSFWKTQVGGLIRSAERLGDCRP
                     VIAERLLDIDGEDSSDASAPDPKGLGTGRLAAAGFDVPRLYRGAMLTLGRDDEPALRR
                     ALEAAPAPLRTHALARLAHRGREDWARRVYAIEGLAATGDLKTLTTLAGMLDSLTPAD
                     QARVARTIGEAAQRPPFDPCGPDQTFGGGGGSNMWTREVPMLGRRCETSLTVAQSNTL
                     ASALLPLVEHREEQVQSAAIEALGKLAVQSTRPTLRKLARPRPPACADDRTCAAQRRH
                     QAAVEAVATLDRAPTDPVWRKYDGKRGQ"
     gene            3416..4018
                     /locus_tag="VGB85_12770"
     CDS             3416..4018
                     /locus_tag="VGB85_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134956.1"
                     /translation="MTEPPTTPHWRQIHALGLPSGSVRALLALGVVGIVCGLLLRAPE
                     QPLPEHLESLLFVVLGHYFAARGQAAKDPAGGPPPLFLPRGSVRLLLVAALITVGVLV
                     VRQGGLQLHRGLFTLLLVFGFSLGLLVTRVVTFWQRRRPGHNRWFEDLRAVIGIGAVA
                     ALGVHTFVGVLPGPEVIGFGNLGPAQALAGLVGFYFGSRS"
BASE COUNT          518 a         1371 c         1644 g          563 t
ORIGIN      
        1 cgggtcggcg gtgaggggcg cgggctggcc ctcgaccggg cgcgcgaggc tgaagtcgag
       61 gacgcggacg cggccgtcgt cgccgacgag gatgttgtcg ggcttgacgt cgcggtggac
      121 gacgcccgcg gcgtgcgcgg cggccaggcc ggcgccggcc tgctggaaca cgccaaggac
      181 gtcccgccag cgccgtggtt tggcggcgag ccactcgccg agggtgtggc cgcggaccaa
      241 ctccatggcg atgtacacgg cctcgccgac ggagccgacc tcgtagacct ggacgacgtt
      301 ggggtgggag acgcgggcga gggcctgcgc ctcgcgcagc aggcgggtct cgccgatcga
      361 ggctcgctcg ctgcgcagcc cgggcagcac gagcttgatc gccagcttgc gctcgagctc
      421 ctcgtcgtag gcggcgtaca cgacgcccat gccgccctca ccgagcttgc gcagcacgac
      481 gaagcgcccg atccgcgcga gttgctccaa agtccccgca atatggaccg tcggcgcgtc
      541 aggtttcaag gtgcgcggcc gcgcgaggcg gatcggacgt acactgtcca catcacgatg
      601 acgacgctgc cgctgctcgc ctgcgtggat gtcgactacc gcggagccgg ggcggtggcg
      661 gcggcgatcc tgttcctcga ctggagcacc ccgcacgcgc tcgcggagca cgtggtcgcg
      721 gtcgaccgcg tcgcgccgta ccggcccggg gcgttctacg agcgcgagct gccgtgcatc
      781 atggccgtgc tggcgcgcag cacctggccc attgccacga tcgtggtcga tggccacgtg
      841 tggctggggc ccgaggagcc cggcctcggc gcccacctgt acctggcact cggtggccac
      901 atcccggtcg tcggtgtggc caagagcccc taccgcgggg cgccggcggc cgagctgctg
      961 cgcggcgaca gccagcgacc gctgtatgtc acggccgccg gcgtcgaccc ggagatcgcg
     1021 gccgacaaca tccgccgcat gcacgggccg catcgcctgc cgacgctgct caaggcggtc
     1081 gacagcctgt gtcgtcgcgg gtgatcaggc gcggatcagc gcgcgccatt cgtcggactc
     1141 ggcgacggtc tgcagcacgt gattgcgacg ctcgaggaag cggcgcaggt aggcggtcag
     1201 gaacacggcg tcggcgaggc ggccgagcgg gccgagcggc gagctgaagt cgaagacgtc
     1261 gcgcatcacc gtgcggccgg cgagctcctc gaagtaatga tcgtgatcga agcgggcgaa
     1321 cgcgccgcgc accatcgagt cgcggaagtg atgcgggcga tcgtaggcgg agatcgtgct
     1381 ggtcaggcgc tgccacacgc cgaggtgacg cgcgcgccag gtgacctcgt cgccagggcc
     1441 catcaggccg gagcgcacgc cgccgacgat cgtctcgccg gtcgcggcgg tcgagtgcat
     1501 gtgcaagtcg acgctgcgcg cgaggtcgaa gcagcggtcg accggcgcgt cgatctcggt
     1561 ggtgagacgg atgaccggca tcactgtccg cgcttgccgt cgtacttgcg ccacacgggg
     1621 tcggtcgggg cgcggtcgag ggtggcgacg gcctcgacgg ccgcctgatg gcggcgctgc
     1681 gcggcgcagg tgcggtcgtc agcgcaggcg gggggcctgg gtctggcgag cttacgtaga
     1741 gtcgggcgtg tggactgtac ggcgagcttg ccgagcgcct cgatcgccgc gctttggacc
     1801 tgctcctcgc ggtgctcgac gagcggcagc agggcgcttg cgagggtgtt cgactgcgcg
     1861 accgtcagcg aggtctcgca gcggcggccg agcatcggca cctcgcgggt ccacatgttc
     1921 gagccgccgc ctccgccgaa ggtttggtcg gggccgcagg ggtcgaaggg ggggcgctgc
     1981 gcggcctcgc cgatcgtccg ggcgacgcgc gcctggtcgg ccggggtcaa gctgtcgagc
     2041 atgccggcga gcgtggtcag ggtttttaga tcgccggtcg cggcgaggcc ttcgatcgcg
     2101 tacacgcggc gggcccagtc ctcgcgaccg cgatgcgcga ggcgggcgag ggcgtgggtt
     2161 cgcagcggcg caggggctgc ctcgagggcg cgacgcaggg cgggctcgtc gtcgcggccg
     2221 agggtcagca tggcgccgcg atacagccgc gggacgtcga agccggcggc ggcgaggcgc
     2281 ccggtgccga ggcctttcgg gtccggcgcg gacgcgtcgc tcgagtcctc gccgtcgatg
     2341 tcgagcaggc gctcggcgat cacggggcgg caatctccga gccgctcggc gctgcggatc
     2401 aggccgccga cctgggtctt ccagaacgac cagtagctcg tgccacacat gccgccgccc
     2461 gggtcggcgc gacggttgag gtcgtacgcg agggtgagct cgccggtcag ctcggcgagc
     2521 tcggcgaggt cgcgcatgac gaagctgtga cgcgcgccgc cccacgcctg atcggggtcg
     2581 atggcgccgt cgagcgcgcc gggataaccg aaggaggcga ggtagcgccg gccagccgcg
     2641 atcgcaccgg cgaggtcgcc cgcgtgcgtg gcgagctgga gctgcgcgag cgcgtcggtg
     2701 cgaagctggt cctgcgcgcc gggtttgccg ggttgtccag ggatgctggt gctcgcctcg
     2761 gccggcgcac cgacctcgcg gccggcgtcg ccgagtgcgg cgaggaagta ggcgaggtcg
     2821 tcgcgctcgc gcggctggag gggctccgcc gtgagcacgc cggcccgcac gcgcacgacc
     2881 aggaaaccgg cgaggccggc ggcggcgcgg ccgagctcgg cgggcgaggg cgggtcgcag
     2941 cgggcgcgcg ggagggcctc gaccatcgca cgtgtgcgca ggtcgtcgag cgcctgcgag
     3001 gccgcgaagc tgccgcgccg tgaggcacgc agcaggctgc cggcgtggcg atcaccgccg
     3061 agtcggccga gcgcccgggc gaggcggtcg accaggtcgg tgtggggttg cagcggcgcg
     3121 cacgggccag gcccggggca ggccgaggtc agggcgtcga gctgcgcggc cagcaggtcg
     3181 gggtcgaggc ccgcggtgcg ggcgatcgcg ccgttgtaat cgaaggtgcg ggggccgagc
     3241 ttcgcggcgc gcagggtggt gtcggcggcg gtgacggcga gctcgagctt gctcgtcggg
     3301 gcgctcgcgg ccgcagcagc cggcgcagcg agggcgagca cgagggcgaa ccaggggacg
     3361 ggcatggcgg cgagggacag cgacgaaccg cgaaggttcc aggcatactg ccacgatgac
     3421 cgagccgccg acgaccccgc actggcggca gatccacgcg ctcgggctgc cgtccggcag
     3481 cgtgcgagcg ctgctggccc tcggagtcgt cgggatcgtg tgcgggctgc tgctgcgcgc
     3541 gcccgagcag ccgctgcccg agcacctcga gagcctgctg ttcgtggtgc tcgggcacta
     3601 cttcgcggcg cgcgggcagg ccgcaaagga cccggcaggc gggccgccgc cgctgttcct
     3661 cccgcgcggc agcgtgcgcc tgctgctggt cgccgcgctg atcacggtcg gcgtgctggt
     3721 ggtccggcaa ggcggcctgc agctgcaccg cgggctgttc acgctgctgc tggtgttcgg
     3781 cttctcgctc ggcctgctgg tgacgcgggt ggtcacgttt tggcaacgcc ggcgcccggg
     3841 ccacaaccgc tggttcgagg acctgcgcgc ggtgatcggg atcggcgcgg tcgctgcgct
     3901 gggggtgcat acctttgtcg gggtgctgcc ggggccggag gtgatcggct tcggcaacct
     3961 cggccccgct caggcgctcg ccgggctggt cggcttctac ttcggctcgc gttcgtgatg
     4021 accgctggcg cgcccgcgcg atcgggccca cgcttggtgg atgcagatcg acatccacta
     4081 ctgcacgcgc tgaaac
//
LOCUS       DASWNJ010000763         3403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_579407, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000763 DASWNJ010000000
VERSION     DASWNJ010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579407"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /locus_tag="VGB85_12775"
     CDS             complement(<1..1290)
                     /locus_tag="VGB85_12775"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0134957.1"
                     /translation="MGAVWRGVHLKLERVVAVKILDETLQLRQDGRERFIREARALAL
                     LAHRNVVAIYDCDELPDGRLYLCMELLAGKTLRDIIKRRERLDPLEVIDLGMQVCDAV
                     QAAHDHGILHRDLTPSNIIRLDDAAKTIKVIDWGLCKYLDLFYVRAPLKYAAPPGSRL
                     VTPLGCRFGTPEYMAPEMILRDKPGPPSFCTDVYALGVVLYELLTGRHPFVPGDRKLP
                     RPIRDLLPGFDNHDLEAALRAALRYLPDDRTPTMAELREGLDLARECLLAQREDVAAA
                     GIGPVLDPAPARSEQAAAPVVTPAQVSTSAQQEHVEAVASMTSGEIATAKAGPDAAAV
                     ATKEVPAPPAELARVVPLPPRGHSARTVLAAVLGLAAGVVGTLASQPDPGVALAPERT
                     VLTARLELARASAAEQRADRCEAEAARHTPAEVLTTPP"
     gene            1213..1602
                     /locus_tag="VGB85_12780"
     CDS             1213..1602
                     /locus_tag="VGB85_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134958.1"
                     /translation="MERLIKDLHRDDALELEVDPAPHGPHAALADLFEDLDPTGEDAL
                     WVLLGYEVRGVLVFVEEETVRVVGLLVVDLVLVVPVDLLRLALVGRVVGVSLARGGAR
                     VVQPFCAHVALRPGDTTTHRLARSRVA"
     gene            2158..3237
                     /locus_tag="VGB85_12785"
     CDS             2158..3237
                     /locus_tag="VGB85_12785"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEY0134959.1"
                     /translation="MPRRLVILLGLTGGCLFADPGPPASGDGDAQTSTASEVNGSTEP
                     PMSTDAPTTEDTGSPTGDCTWEQEMTEPRPGKRSDAALALAPGGTEALLYGGRVGLLG
                     PDLEDTWRFDGERWRLVLADVQGGGKKEQPGPRRGHAMAHDAARDRVLLFGGEYGGPN
                     VKFADSTWIHDGSRWNKGHDGPSPRAFTALADLPHSAVVVLFGGRTDEGVNAETWTWN
                     GQDWLQRDLEEAPSPRYGHTMILDGSGDVLLYGGCSELLCGDPLDDTWRFDGERWERL
                     DTGAPEGLRSGGMVQDTARALTLRLGEGASWRWTGTTWSPADAAEPAVGHFALADLPG
                     TGVVLFGGLTAQTIESDETWVLRCS"
BASE COUNT          506 a         1158 c         1188 g          551 t
ORIGIN      
        1 cggcggcgtg gtcaggactt cggccggcgt atgcctcgcc gcctccgcct cgcagcggtc
       61 ggcgcgttgc tccgccgcgc tcgcccgcgc cagctccagc cgggccgtca ggacggtccg
      121 ctccggtgcg agagcaacgc ccgggtccgg ttgcgaggcg agcgtgccga cgacacccgc
      181 cgcgagcccg aggacagccg cgagcacggt ccgcgcggaa tgccctcgcg gcggcagcgg
      241 gacgacgcgc gccagctcag ccggtggcgc ggggacctcc ttcgtggcga ccgctgccgc
      301 gtccgggcca gccttggccg tcgcaatctc ccccgaggtc atgctagcca ctgcctcgac
      361 gtgctcctgc tgggccgacg tggagacctg cgccggggtg acgaccggcg cggccgcctg
      421 ctcggaccgg gcgggcgcgg ggtccagtac gggccctata cctgccgccg cgacgtcctc
      481 gcgctgggcc agcaagcact cgcgcgccag atcgagcccc tcgcgtagct cggccatcgt
      541 cggcgtccgg tcgtcgggta gatagcgaag ggcggcccgg agagcggcct caaggtcgtg
      601 gttgtcgaag cccgggagca agtctcggat cggccgcggt agtttgcggt caccgggtac
      661 gaacgggtgc cgcccggtaa gcagctcgta gagcaccacc ccgagcgcgt acacgtcggt
      721 gcagaagctc ggcgggcccg gcttgtcgcg caggatcatc tccggcgcca tgtactcggg
      781 cgtgccgaac cgacagccga gcggcgtcac gaggcgggag cctggcggag cggcgtactt
      841 caggggggct cggacgtaga aaaggtcgag gtacttgcag agtccccagt cgataacctt
      901 gatcgtcttc gcggcatcat caaggcggat gatgttcgac ggcgtgaggt cgcggtgcag
      961 gatgccgtgg tcgtgcgccg cctgaacggc gtcgcagacc tgcatgccca ggtcgatcac
     1021 ttcgagcgga tcgagacgct cacggcgctt gatgatgtcg cgcagcgttt tgcccgcgag
     1081 cagctccatg cacaggtaca gcctgccatc cggtagctcg tcgcagtcgt agatcgcgac
     1141 gacgttgcgg tgggcgagca gggccagcgc acgggcctcg cggatgaacc gctctcggcc
     1201 gtcctggcgg agttggagcg tctcatcaag gatcttcacc gcgacgacgc gctcgagctt
     1261 gaggtggacc ccgcgccaca cggcccccat gccgccctcg ccgatctttt cgaggatctc
     1321 gatccgaccg gcgaggacgc gctttgggtg ctcctcgggt acgaggttcg gggcgttctc
     1381 gtcttcgtcg aggaagagac tgtccgcgtc gtcggcctcc tcgtcgtcga cctcgtcctc
     1441 gtcgtccccg tcgacctcct ccgcctcgcc ctcgtcggtc gcgtcgtcgg cgtcagtctg
     1501 gctcggggtg gtgctcgcgt cgtccagccg ttctgtgcac atgtcgccct ccgacctggt
     1561 gacactacca cgcatcggct tgcccgctcg cgggtagcct aacgcgcata accgcgcaga
     1621 tggcctgaac aggcgcctag atcgtgtagg agcgtaccta caagcgtatg taaccgtcga
     1681 ccaagactat gcgaagccgt ctcctgcaag cgacctctgt cgggtcaggg tgtgcggggc
     1741 tcaagcttcg cctcgaccac cttggtctca gcttttcttc tcgtcggctg gatctgctcg
     1801 cggcggtgcg actcttcgat cagggcgagg atccgatcga gagtctcgcc gcacgcacca
     1861 acccgtcgct tcgcctggtc ctcggcggcc ctccggtagc ggtcaacttc ggcggcccca
     1921 tccgatcgcg cggcggatcc tgcgccctgt cgcgtagggt caatcgccag ccgctctcgg
     1981 ttatgaccta cgttcccatg cctgcgacag cccgaggacg aggcagccag cgcgacgacc
     2041 gcgcgccgtc ctcacccatc agtcacggct atgttggtgt cgggatgcga cgcgaagatg
     2101 tctgagtgcg aggcggggcc accgcagaag tctggcgagt gccggctgtt ccatcccatg
     2161 cctcgccgcc ttgtcatcct tctcggcctg accggcggat gtctgttcgc cgacccgggc
     2221 ccgcctgcga gcggtgacgg cgatgcccag acgagcacag cgagtgaggt caacggcagc
     2281 accgagccgc ccatgagcac ggacgcgccg acgacggagg acactggatc gccgactggc
     2341 gactgcacct gggagcagga gatgaccgag ccgcgccccg ggaaacgcag cgacgccgcc
     2401 ctggcgctcg cgcccggggg cacggaggcg ctgctgtacg gcgggcgggt cggcctcttg
     2461 ggacccgacc tcgaggacac gtggcgcttt gacggcgaac gctggcggct cgtgctcgcc
     2521 gacgttcagg ggggcggcaa gaaggagcag cccgggccgc ggcgcggcca cgcgatggcg
     2581 cacgatgcgg cgcgcgatcg ggtgctgctt tttggcggcg agtacggcgg ccccaacgtg
     2641 aagttcgcgg acagtacctg gattcatgac gggtctcgct ggaacaaggg ccacgatggg
     2701 ccctccccgc gcgccttcac agcgctggca gacctcccgc acagcgcggt ggtcgtgctg
     2761 ttcggcggtc ggactgatga gggcgtgaac gccgagacct ggacgtggaa tggccaggac
     2821 tggttgcagc gcgatctcga agaagctccg tcgccgcgtt acggccacac gatgattctc
     2881 gatggctccg gggacgtgct gctatacggg ggatgcagcg aactgctgtg tggggaccca
     2941 ctcgacgaca cctggcgttt cgacggcgag cgctgggaac ggctcgacac cggcgcaccc
     3001 gagggcctgc gctctggcgg aatggtccaa gacaccgccc gtgccctgac cttgcgcctc
     3061 ggcgaaggcg cgagttggcg atggaccgga acgacgtggt cacccgcaga cgcagcagag
     3121 cccgccgtcg gccatttcgc gctcgcagac ctgcccggca ccggagtggt gctattcggc
     3181 gggttgacgg cacagacgat cgaaagcgac gaaacctggg tccttcgctg ctcgtaagat
     3241 acgcgcgtga tgcccccgga aggcgggcat ccatggtctg gcatggccac ccgctcagcc
     3301 cggcgtgatc ggctcgcagc agccggtctg gcagaacgtg ccctccgggc agtcggagtt
     3361 gccctcctga tcgagcttgc aaggattcgc gcgatcgcag gtc
//
LOCUS       DASWNJ010000764         1549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_544727, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000764 DASWNJ010000000
VERSION     DASWNJ010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544727"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /gene="dnaK"
                     /locus_tag="VGB85_12790"
     CDS             <1..1308
                     /gene="dnaK"
                     /locus_tag="VGB85_12790"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEY0134960.1"
                     /translation="GTFDVSLLEIGDGVIEVKATSGDNRLGGDDWDQRVVEHLVKQFA
                     LSNNGVDLSGDKMAMQRLREAAEKAKIELSASQSTSINLPYITASPEGPLHLDVTLTR
                     AEFQRLTQDLLDRCKTPFNQAVKDAGISVGQIDHVVLVGGSTRMPAVSDLVRELTGKE
                     PNKGVNPDEVVAVGAALQAGVLKGEVKDVLLLDVTPLSLGIETKGGIMTKLIERNTTI
                     PTKRSEVFTTADDNQPSVQIQVFQGEREMAAYNKKLGMFELTGLPPAPRGVPQIEVTF
                     DIDANGIVHVSAKDLGTGKEQRMTISGGSALPKDEVERMMREAEEYAEEDRKRREEVE
                     TRNQAEALVFQTEKFLGENGDKLPAEGKAEAEEALTELRSALGGSDTAAVKAAAEKLA
                     TKSQELGGALYAQQAADAPTAPGAAGAADDGVVDAEVVEDEGK"
     gene            1305..>1549
                     /locus_tag="VGB85_12795"
     CDS             1305..>1549
                     /locus_tag="VGB85_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134961.1"
                     /translation="MTAPDRDERIVDDPDQVVVRDKRRLDPESGAVRTPTQAAADEHP
                     DAEAPVGEQPDRVAELTADLQRLSAEYANYRKRVERDR"
BASE COUNT          281 a          563 c          532 g          173 t
ORIGIN      
        1 ggcaccttcg acgtgtccct gctcgagatc ggcgacggcg tcatcgaggt caaggcgacc
       61 tccggcgaca accgcctcgg cggggacgac tgggaccagc gcgtcgtcga gcacctggtc
      121 aagcagttcg cgctgtccaa caacggcgtg gacctgtccg gcgacaagat ggccatgcag
      181 cgcctgcgcg aggcggccga gaaggccaag atcgagctgt cggcgtcgca gtcgacgagc
      241 atcaacctgc cgtacatcac ggcgagcccc gagggcccgc tgcacctgga cgtcacgctg
      301 acgcgcgccg agttccagcg cctcacccag gacctgctcg accgctgcaa gaccccgttc
      361 aaccaggcgg tcaaggacgc cggcatctcc gtcggccaga tcgaccacgt cgtgctcgtc
      421 ggcggctcga cccgcatgcc cgccgtgtcc gacctggtcc gcgagctcac cggcaaggag
      481 cccaacaagg gcgtcaaccc ggacgaggtc gtggccgtcg gcgccgccct gcaggccggt
      541 gtcctcaagg gcgaggtcaa ggacgtcctg ctgctcgacg tcaccccgct gtccctcggc
      601 atcgagacca agggcggcat catgaccaag ctcatcgagc gcaacaccac catcccgacc
      661 aagcgctccg aggtcttcac gaccgccgac gacaaccagc cgtcggtgca gatccaggtc
      721 ttccagggcg agcgcgagat ggccgcctac aacaagaagc tcggcatgtt cgagctgacc
      781 ggcctgccgc cggcgccgcg cggcgtgccg cagatcgagg tcaccttcga catcgacgcc
      841 aacggcatcg tgcacgtctc cgcgaaggac ctcggcaccg gcaaggagca gcgcatgacg
      901 atctccggcg gcagcgcgct gccgaaggac gaggtcgagc gcatgatgcg cgaggccgag
      961 gagtacgccg aggaggaccg caagcgccgc gaggaggtcg agacccgcaa ccaggccgag
     1021 gcgctcgtct tccagaccga gaagttcctc ggggagaacg gcgacaagct gcccgccgag
     1081 ggcaaggccg aggccgagga ggcgctcacc gagctgcgct ccgccctggg tggcagcgac
     1141 accgccgccg tcaaggccgc ggcggagaag ctcgcgacca agagccagga gctcggcggc
     1201 gccctctacg cccagcaggc ggcggacgcc cccaccgcgc ccggcgcagc cggcgctgct
     1261 gacgacggtg tcgtcgacgc cgaggtcgtg gaggacgagg gcaagtgacg gctcctgacc
     1321 gcgacgagcg gatcgtcgac gaccccgacc aggtcgtcgt ccgcgacaag cgacggctcg
     1381 acccggagtc cggtgccgtc cggacgccga cgcaggctgc ggcggacgag caccccgacg
     1441 ccgaggcacc tgtcggcgag cagccggacc gggtggcgga gctgacggcc gacctccagc
     1501 ggctgtccgc cgagtacgcc aactaccgca agcgcgtcga gcgcgaccg
//
LOCUS       DASWNJ010000765         5238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_650234, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000765 DASWNJ010000000
VERSION     DASWNJ010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650234"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="VGB85_12800"
     CDS             complement(<1..1176)
                     /locus_tag="VGB85_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134962.1"
                     /translation="MFRRARLAPLLLCLLVPGAGACKRGGKARPAEAGSAQESAAMGS
                     WDAWLDLAPLLEVARSHAQPKTVAAIEQASKLLRDGKARTADKGLAMVADSEGRHWIS
                     VARADLAALYFTRCIRGVVWRLEDLTSKSTPTRKSDFSEDTKIEPGDVSVEALLTNLD
                     GAVAAKEPALQIQARIARARVTAYSARCAANEEVARMSEGILKSDLATLAAEGHLTPD
                     LAYLWAGVQMAEYSGAAAKPFLLLAKDGGYTDPTVTYMLGVIALEQRDLGPADAYAVE
                     AAKTYAKLGDTDQQAQSLFLRGEVARARKDVPAARKHYEAAQKLAPGHPAALLGVARL
                     VLEAEGSTRAIGYIHKALPQIVLTGPLQGEKLRIASSNLEALSVLATESEIASVCRDA
                     "
     gene            1329..2336
                     /locus_tag="VGB85_12805"
     CDS             1329..2336
                     /locus_tag="VGB85_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134963.1"
                     /translation="MRHPVKTTTLGLLSTLLVGAAVFACNPAVAEAHRPWHNTEHVHP
                     QPAQDVSVTLESPGGGAVQTFWHGGSLYAAGHNGARYNLRLQNNTGERVEAVVTVDGR
                     DVVSGQLGNYKSQRGYVMDPYGSVVIEGFRQSLDQVAAFRFTDIGNSYSARRGSGQHV
                     GVIGVAVFKEYQPRRRPPPTPVPIATRPYYEPGDGDAGGYDYKRESSAPAAKRAEAPA
                     PSSAPPHGGGQVTTAYDRDDAVYEEAEARTRSTKLGTEYGESTYSSVREVSFTRKHKR
                     RPDALLTIYYDSLEGLRARGVPVDPPVYHHPPPLYPPQPFPNDGERHNQYAPPPPPRR
                     Y"
     gene            complement(2557..3453)
                     /locus_tag="VGB85_12810"
     CDS             complement(2557..3453)
                     /locus_tag="VGB85_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEY0134964.1"
                     /translation="MSTQFIPSGLAFPKRSTPAAAPSPVVEDDESTYKLHRRFDRMGR
                     LVGDGGMAKLFGAHVMVIGLGGVGSWAAESLARSGVGKLTIVDFDLICVTNANRQLHA
                     LRGTTGKQKAAVMAERLRLINPRCEVVAEPIFYNSESSAALLAHAPDLVIDAIDNLAA
                     KTHLIATCRTRGIPLVVCGGASGRMDPTQIRIADLNAVGHDPFLAATRKLLRRDYEFP
                     SEKTAWGISAVHSLELPADPVELHYDNGEGFKCVCPHGGNDLHGCMHRNVIYGTASFV
                     TGAFGLACASAAVRTLLGQPVG"
     gene            complement(3540..3836)
                     /locus_tag="VGB85_12815"
     CDS             complement(3540..3836)
                     /locus_tag="VGB85_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134965.1"
                     /translation="MKIAGLVLLLLALVLGGLWIAYVRAPEPELVCEHKIAITLAEVG
                     DQHGQAVDNLIDQLKLQCVKEKRKLLQLRGKIAYARQAKCILAATTLSAAERCG"
     gene            complement(3859..4458)
                     /locus_tag="VGB85_12820"
     CDS             complement(3859..4458)
                     /locus_tag="VGB85_12820"
                     /inference="COORDINATES: protein motif:HMM:NF016286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HEY0134966.1"
                     /translation="MVHPVQSVIESLYAAGRCPRLHVNATHASVVCPEEIRHQWKERL
                     IIDLDSSYPLDLTFGQTGLAANLSFGGLVTRCSFPWEAIYVVMDRATGRGIVLDRHVP
                     ESVRRQSGAAAPAARLDSDGESTPERPELRAVSTEPDTIEVSETPASAAASAASAAAA
                     VPGTAGAEPAEADKTATPATDETQVKKRRAAFRVIDGGG"
     gene            4726..4902
                     /locus_tag="VGB85_12825"
     CDS             4726..4902
                     /locus_tag="VGB85_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134967.1"
                     /translation="MKLHDHCDCTCHGAASGGPSLGPGLTAPPSTGFFVVSLSLTLLL
                     CGLISTGVIPLRFW"
     gene            complement(4924..>5238)
                     /locus_tag="VGB85_12830"
     CDS             complement(4924..>5238)
                     /locus_tag="VGB85_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0134968.1"
                     /translation="TQQKLVEMHNEIGKGLLLAYHFGRLKQDGQLSPVQVSYLKRDNV
                     RMALEAARTARSMFGGNGIMGEFHVMRHMCNLETVYTYEGTHEIHTLALGRALTGLDA
                     FS"
BASE COUNT          747 a         1767 c         1948 g          776 t
ORIGIN      
        1 cgcgtcacga cacaccgacg cgatctcgct ctcggtcgcc aggaccgaca gcgcctcgag
       61 gttgctcgac gcgatgcgca gcttctcgcc ctgcagcggc ccggtcagta cgatctgagg
      121 cagcgccttg tggatgtagc cgatcgcgcg ggtgctgccc tcggcctcga gcacgagacg
      181 ggccacgccg agcagcgcgg cggggtggcc gggcgcgagc ttctgcgcgg cctcgtagtg
      241 cttgcgtgcg gccgggacgt ccttgcgggc gcgcgcgacc tcgccgcgca ggaacagcga
      301 ctgggcctgc tggtcggtgt cgccgagctt ggcgtaggtc ttggcggcct cgacggcgta
      361 ggcgtcggcc gggccgaggt cgcgctgctc gagggcgatg acgccgagca tgtacgtgac
      421 ggtcgggtcg gtgtagccgc cgtccttggc gagcagcagg aatggtttgg cggcggcgcc
      481 ggagtactcg gccatctgca cgccggccca cagataggcg aggtcggggg tgaggtggcc
      541 ctcggcggcg agggtggcga ggtcgctctt gaggatgccc tcggacatgc gcgcgacctc
      601 ctcgttggcg gcgcagcggg ccgagtaggc ggtgacgcgg gcccgcgcga tgcgggcctg
      661 gatctgcagc gcgggctcct ttgcggcgac ggccccgtcg aggttggtca gcagggcctc
      721 gacggagacg tcgccgggct cgatcttcgt gtcctcgctg aagtcgctct tgcgggtcgg
      781 cgtggacttc gaggtcaggt cctctaggcg ccaaacgacg ccgcggatgc atcgcgtgaa
      841 gtagagggcg gcgaggtcgg cccgcgcgac ggagatccag tggcgaccct cgctgtcggc
      901 gaccatcgcc aggcccttgt cggcggtcct ggccttgccg tcgcgcagca gcttcgaggc
      961 ctgctcgatc gctgcgaccg tcttcggctg ggcgtggctg cgcgcgacct cgagcaaggg
     1021 cgccaggtcc agccaggcgt cccagctgcc catcgccgcg ctctcctgcg ccgagccggc
     1081 ctccgcgggc cgagccttgc ctccgcgctt gcaggccccg gccccgggga cgagcaggca
     1141 caggagcagc ggagcgaggc gggcgcggcg aaacatggcg ccggaggcta cgccagcgcg
     1201 cgcccggggg ccaccggcaa gcggggcacg aggccgcttt ttcgcggtgt aaggcgcccg
     1261 cgggggccag cgttcgggca gatcgaagcc cccggacgcc cgttccggcc ggccccggag
     1321 acccgaccat gcgccacccc gtgaagacca ccaccctcgg cctgctgtcg accctgctcg
     1381 tgggcgccgc cgtgttcgcg tgcaaccccg cggtcgccga ggcccatcgc ccgtggcaca
     1441 acaccgagca cgtccacccg cagccggccc aggacgtgtc agtgacgctc gagagccccg
     1501 gcggcggcgc ggtccagacg ttctggcacg gcggctcgct gtacgcggcc ggccacaacg
     1561 gcgcccgcta caacctgcgc ctgcagaaca acaccggcga gcgcgtcgag gcggtggtga
     1621 cggtcgacgg ccgcgacgtg gtctccggcc agctcggcaa ctacaagtcg cagcgcggct
     1681 acgtgatgga cccctacggc tcggtcgtga tcgaaggctt tcgccagtcg ctcgaccagg
     1741 tcgccgcgtt tcgctttacg gacatcggca acagctactc ggcgcgtcga ggcagcgggc
     1801 aacacgtcgg cgtgatcggt gtggcggtct tcaaagaata ccagccgcgc cgccggcccc
     1861 cgccgacgcc ggtcccgatc gccacgcggc cgtactacga gcccggtgac ggcgatgccg
     1921 gtgggtatga ctacaagcgc gagtcgagcg cgcccgcggc caagcgcgcc gaggcgccgg
     1981 ctccgagctc ggcgcctccc cacggcggcg gccaggtgac gaccgcgtac gaccgcgacg
     2041 acgctgtgta cgaggaggcc gaggcccgca cgcgcagcac caagctcggc acggagtacg
     2101 gcgagtcgac ctacagctcg gtgcgcgagg tcagcttcac ccgcaagcac aagcgccgcc
     2161 ccgacgcgct gctgacgatc tattatgaca gcctcgaggg cctgcgcgcg cgcggggtgc
     2221 cggtcgaccc gcccgtctat caccacccgc cgccgctcta cccgccgcag ccgttcccga
     2281 acgacggcga gcggcataac cagtacgcgc ccccgccccc gccgcggcgg tactgagaag
     2341 ttgaaaacgg gacaggcgcc gcccgcgagg gtgagcgcct gtcggcactt tcaccggcag
     2401 aggagttcct gccggcatgg cgggctcacc ggcgcgggcg gaggcgcggc tggcctgtcc
     2461 tcggggacat gtgccttgca gctgctggcg agcgcgagca gcagcgcgaa gcgacacgag
     2521 ccagccggca tgcacggtag cacggcgccc gcgcgctcag ccgactggct ggccgagcag
     2581 cgtgcgcacg gccgcggacg cgcaggcgag gccgaacgcg ccggtcacga agctcgccgt
     2641 tccgtagatg acgttgcgat gcatgcaccc gtggaggtcg ttaccgccgt gcgggcacac
     2701 gcatttaaaa ccctcgccat tatcgtagtg cagctcgacg gggtcggccg gcagctcgag
     2761 cgagtgcacc gcggagatgc cccacgcggt cttctcggag gggaactcgt agtcgcggcg
     2821 cagcagcttg cgggtcgcgg cgaggaaggg gtcgtggccg acggcgttga ggtcggcgat
     2881 gcggatctgg gtcgggtcca tgcggcccga ggcgccgcca cagacgacga gggggatccc
     2941 tcgcgtgcgg caggtggcga tcaggtgggt cttggcggcg aggttgtcga tcgcgtcgat
     3001 gacgaggtcg ggggcgtgcg cgagcagggc cgcgctcgac tcgctgttat aaaaaatcgg
     3061 ctcggcgacg acctcgcagc gagggttaat taaacgcagg cgctcggcca tcacggcggc
     3121 cttctgcttg ccggtggtgc cgcgcagcgc gtgcagctgg cggttcgcgt tggtgacgca
     3181 gatgaggtcg aagtcgacga tcgtcagctt gccgacgccg gagcgcgcga gcgactcggc
     3241 cgcccaactg ccgacgccgc cgaggccgat caccatcacg tgcgcgccga acagcttcgc
     3301 catgccgccg tcgccgacga gccgacccat gcggtcgaag cggcggtgca gcttgtaggt
     3361 gctctcgtcg tcctcgacca ccggggacgg ggcggcagca ggagtgctgc gcttggggaa
     3421 cgcgaggccg ctcgggatga actgggtcga catgacggcg cgcagtatag ccatgcggcc
     3481 tgccgaaacc gcggagcttc tcgcgggaaa ggtcttgcgt gcgggcacac cgccactggt
     3541 cagccgcagc gctcggcggc ggacagcgtg gtggcggcga ggatgcactt ggcctggcgg
     3601 gcgtaggcga tcttgccgcg aagttggagc agcttgcgct tctccttgac gcactggagc
     3661 ttgagctggt cgatcaggtt gtcgacggcc tggccgtgct ggtcgccgac ctcggcgagg
     3721 gtgatcgcga tcttgtgctc gcacacgagc tcgggctccg gggcgcgcac gtaggcgatc
     3781 cacaggccgc cgagcaccag cgcgagcagc agcaacacga ggcccgcgat cttcatgatc
     3841 atttaaaaag aaaagagctc agccgccgcc gtcgatgacg cggaaggcgg cgcgccgctt
     3901 cttgacctgg gtctcgtcgg tcgccggcgt cgcggtcttg tcggcctcgg ccggctcggc
     3961 gcccgcggtg cccgggacag cggccgcggc ggaagccgcg gaggccgctg cggaggcggg
     4021 tgtctcgctg acctcgatgg tgtcgggctc ggtcgagacg gcgcgcagct cggggcgctc
     4081 gggggtggat tcaccgtcgg agtcgaggcg cgcggccggg gcggccgcgc cggactggcg
     4141 gcggacggac tcgggcacgt ggcggtcgag cacgatgccg cggccggtgg cgcggtccat
     4201 gacgacgtag atggcctccc acgggaagga gcagcgcgtc accaggccgc cgaaggacag
     4261 gttggcggcg aggccggtct gaccgaaggt gaggtcgagc gggtagctcg agtccagatc
     4321 gatgatcagg cgctccttcc actggtgccg gatctcctcc gggcaaacca cggaggcgtg
     4381 cgtcgcgttg acgtgcagcc gagggcagcg gcctgccgcg tacagcgact cgatgacact
     4441 ctggaccggg tggaccatcg cccccgcaac ctagccgaag tcccgggccg tggcgaggcc
     4501 gttgtccgcg atggacagcg tcccgggcac gaaatcgtcg gcatacttcg acccgcaaag
     4561 ccgcgtggat gtgccgcgct gcgccgcgcc gatgcccagg gaatgccctc gtggtgccgc
     4621 tgccgggcgg atgagtcgcc tgcgagccct gactcacatg cccgggatcg ccggggtcgc
     4681 ttgcttatgc ggcctgcccg cgtgctaaag tccggttgcg tccgtatgaa gcttcatgat
     4741 cattgtgact gcacgtgcca cggcgcggcc tccggcgggc cgagcctcgg gccgggattg
     4801 accgcgccgc cgtcgaccgg cttcttcgtg gtgtcgctgt cgctgacgct gctgctgtgc
     4861 ggcctgatct cgaccggcgt gatcccgctg cgcttctggt gaccgcttcg gtctgcgacg
     4921 ccctcagcta aacgcgtcga ggccggtgag cgcgcgtccg agcgcgagcg tgtggatctc
     4981 gtgggtgccc tcgtaggtgt agaccgtctc gaggttgcac atgtgccgca tgacgtgaaa
     5041 ctcgcccatg atgccgttgc cgccgaacat cgagcgggcg gtgcgggcgg cctcgagcgc
     5101 catgcggacg ttgtcacgct tgaggtacga gacctgcacc gggctgagct ggccgtcctg
     5161 cttgagccgg ccgaagtggt aggcgagcag caggcccttg ccgatctcgt tgtgcatctc
     5221 gaccagcttt tgctgggt
//
LOCUS       DASWNJ010000766         2292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_507351, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000766 DASWNJ010000000
VERSION     DASWNJ010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507351"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1499)
                     /locus_tag="VGB85_12835"
     CDS             complement(<1..1499)
                     /locus_tag="VGB85_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134969.1"
                     /translation="MRRSLVYVGIFAVALATLMLEILLTRITSVIAWYHLAFFVIALA
                     MLGMTAGAVIVFLWPRVFADVPRRLFDSALGFAALAPPAFAWTMSGPLLPVTDLMGFV
                     ALLGLGLVLALPFTVVGVTLTLALTRAGLPPGRAYGVDLLGAAAGAVLVIPVLDAIDA
                     ASAVLVASAIAAVGSLAFALSMSGGTGRKGQVVAGMLAVVWAGAAWFNAAAEVPPLRP
                     AWVKGVHEDYGAHAWVGWNTHSRVTVSPTVTAPPMLWAAGRNTPPEQLRPIAQRIVKI
                     DGAAATAMAQAGGDIGYLEWDVATFAHQLRPSGPAAVIGVGGGRDVLAAARAGHWPIV
                     GVELNAKIVSLHREAMAEFSGVAAIAGTELVVDEARSFFTRDARQYEVVTMSLIDTWA
                     STGAGAYSLSENGLYTVEGWLAFMRRLTPTGIFTVSRWYKSDSPGETGRMLALAMETL
                     WQLGASDPRAHMILLQQGSVATLLVSPSPFSAQDLDTMQREAVRLGFNMV"
     gene            complement(1537..>2292)
                     /locus_tag="VGB85_12840"
     CDS             complement(1537..>2292)
                     /locus_tag="VGB85_12840"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY0134970.1"
                     /translation="LLLEQVVARCEGVGSVLVLDEVVDIGAACGVLAGWDGRYDVDSV
                     GAVVWREFLGTYGIGSLTDYGPLLAVGFDPEDPIETPNTLVDPPAPGEEDKVLVSLGR
                     GVLTLAQAGVALDSPLGAVQKAPRADKQYGVPGGQGRDGTANVVTYDVLKTTVDPSTP
                     RGEVLSDTTGLTKDGYVVNYGSSFVMALELGQDGPRGQAFLTYGESDDPASKHYADQT
                     VLFSEQKWRPIVFSEADIAADPELATLEVAGGG"
BASE COUNT          367 a          903 c          735 g          287 t
ORIGIN      
        1 accatgttga agccgaggcg caccgcctcg cgctgcatcg tgtcgaggtc ctgcgccgag
       61 aacggcgagg gcgagaccag cagcgtcgcc acgctgccct gctgcagcag gatcatgtgc
      121 gcccgtggat cgctcgcgcc gagctgccac agcgtctcca tcgccagcgc cagcatgcgg
      181 ccggtctcgc cgggcgagtc cgacttgtac catcgcgaca ccgtgaagat gcccgtcggc
      241 gtcagccggc gcatgaacgc cagccagccc tcgaccgtgt acagcccgtt ctccgacagc
      301 gagtacgccc cggccccggt cgacgcccac gtgtcgatca gggacatcgt cacgacctcg
      361 tactgccggg catcacgcgt aaaaaaactg cgcgcctcgt cgacgacgag ctcggtcccg
      421 gcgatcgccg cgacccccga gaactccgcc atcgcctcgc ggtgcaacga aacgatcttg
      481 gcgttgagct cgaccccgac gatcggccag tgtcccgcgc gcgccgccgc gagcacgtcc
      541 cgaccgccac cgaccccgat caccgccgcc gggccggacg gccgcagctg gtgggcaaaa
      601 gtcgcgacat cccactcgag atatccaata tctccgccgg cctgcgccat cgccgtcgcg
      661 gcggccccgt cgatcttgac gatccgctgc gcgatcggcc gcagctgctc cggcggcgtg
      721 ttgcgcccgg ccgcccacag catcggcggc gccgtcaccg tcggcgacac cgtcacccgc
      781 gagtgcgtgt tccagccgac ccacgcgtgc gccccgtagt cctcgtgcac ccccttgacc
      841 cacgccggcc gcaggggcgg cacctcggca gccgcgttga accacgccgc cccggcccac
      901 acgaccgcga gcatcccggc gacgacctgg cccttacgac ctgtccctcc ggacatgctc
      961 agcgcgaacg ccagggaacc gactgcggcg atcgccgagg cgacgagcac cgcgctcgcg
     1021 gcgtcgatcg cgtcgagcac cgggatcacg agcacggccc ccgccgccgc gccgagcagg
     1081 tcgaccccgt acgcgcgccc cggcggcagt ccggcccttg tcagcgccag cgtcagcgtc
     1141 accccgacga ccgtgaacgg cagcgcgagc accagcccga gcccgagcag cgcgacgaag
     1201 cccatcaggt cggtcaccgg cagcagcggt ccggacatcg tccaagcgaa ggccgggggc
     1261 gcgagcgcgg cgaagccgag cgccgagtcg aacagtcgcc gcggcacgtc ggcgaacacg
     1321 cgcggccaca ggaacacgat caccgcgccc gcggtcatgc cgagcatcgc cagggcgatg
     1381 acgaagaacg cgaggtggta ccacgcgatc acgctggtga tccgcgtcag caggatctcg
     1441 agcatcaggg tcgccagcgc gaccgcgaag atgccgacgt acacgaggga ccgccgcatg
     1501 cgcgcaccgt atcaggatcg cgcgccgacg tccgcgtcaa ccgccgcccg cgacctcgag
     1561 cgtcgccagc tccgggtcgg cggcgatgtc cgcctccgag aacacgatcg gccgccactt
     1621 ctgctcgcta aacagcaccg tctggtcggc gtagtgcttc gaggccgggt cgtccgactc
     1681 accataggtc aggaacgcct gcccgcgcgg gccgtcctgg ccgagctcga gcgccatgac
     1741 gaacgacgac ccgtagttga ccacgtaacc atccttcgtc agtcccgtcg tgtcgctgag
     1801 cacctcgccg cgcggcgtcg acgggtcgac cgtcgtcttg agcacgtcgt aggtcacgac
     1861 gttggcggtg ccgtcgcggc cctgcccgcc gggcaccccg tactgcttgt ctgccctggg
     1921 cgccttttgc accgcgccga gcgggctgtc gagcgccacg ccggcctgcg cgagcgtcag
     1981 cacgccgcgc ccgaggctga cgagcacctt gtcctcctcg cccggcgccg gcgggtcgac
     2041 gagcgtgttc ggcgtctcga tcgggtcctc gggatcgaag ccgaccgcaa gcaacggccc
     2101 gtagtcggtc aggctgccga tgccgtaggt gccgagaaat tcgcgccaca ccaccgcgcc
     2161 gacgctgtcg acgtcgtagc gcccgtccca gccggccagg accccacacg ccgcgccgat
     2221 gtcgaccacc tcgtcgagca ccagcaccga gccgacgccc tcgcagcgcg cgaccacctg
     2281 ctcgagcagc ag
//
LOCUS       DASWNJ010000767         3391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_14618, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000767 DASWNJ010000000
VERSION     DASWNJ010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14618"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1080)
                     /locus_tag="VGB85_12845"
     CDS             complement(82..1080)
                     /locus_tag="VGB85_12845"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEY0134971.1"
                     /translation="MGASAGGLAALSEVLRGLPGSFRAAIFIVVHTSPDNPGLLPQIL
                     ARSSALPVAFARDHEPIQPGRVYVAPPDHHLLLKRGYVRVVRGPKENGFRPAVDPMFR
                     TAAHTHGARTIGVVLSGGLNDGTHGLAQIVESGGVALAQDPQDALIPSMPLSAIQNVE
                     VRRVLRAVDIPAALIDLVGEAPSRRPPLPDETPDIAEAGDSLQIHTPPGTPAPYTCPE
                     CGGALWETSDTGKLLRYRCHVGHAYTAEILAAEQEHNLEMALWTALRALEENAALCRR
                     MAERTASSGFAELARRYHTDADHSEERAALLRKVLQSGAVIAMPRPIDAVAELSPP"
     gene            complement(1255..2643)
                     /locus_tag="VGB85_12850"
     CDS             complement(1255..2643)
                     /locus_tag="VGB85_12850"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEY0134972.1"
                     /translation="MAATQSRPRPDPRAPSPEAPLELWGGVECTHNRVGDTYLCQLAR
                     SGHDRRLADLDAIAALGLKTLRYPLLWERTAPRGLDTADWRWTDERFNRLRELGISPI
                     AGLVHHGSGPVDTHLLDPAFPRRLAAYAGAVARRYPWISAYTPINEPLTTARFSGLYG
                     HWYPHQRRDAAFVRALLHQCQATAAAMRAIRVVNPEARLVVTEDLGMTLSTPRLAYQA
                     NFENERRWLSFDLLCGRVDRNHPLHGYLLRHGARPDELAALADEPPPDLLGVNHYVTS
                     VRFLDQRVRRYPAAAIGGNGYQRYADVEAVRVCADFIEPEALFTEVWRRYGRPFAITE
                     AHLGCTTDEQQRWLKEIWDAAQRARLAGADLRAVTAWSLLGAYDWHCLVTRDEGRYEP
                     GAFDLREGPPRPTELARMIDALARGATPDLPALAEPGWWRRPERLVYPPVRVAPRPRV
                     RRVDRPRLAEPA"
     gene            complement(2591..>3391)
                     /locus_tag="VGB85_12855"
     CDS             complement(2591..>3391)
                     /locus_tag="VGB85_12855"
                     /inference="COORDINATES: protein motif:HMM:NF015247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HEY0134973.1"
                     /translation="EIKTAEDVVVSAVGRELYEKFFQGYTRKQWGRDPSQLDKSVTSR
                     VPTRTNTDDRYFTDTFQAMPADGYTKMFEKMLDHPNIKVMTNTCYREVQDMIPFREMI
                     YTGPIDEFFQYRYGKLPYRSLQFKHETHDRAVFQTAPVVNYPNEHAYTRCTEFKYLTG
                     QEHTKTSVVYEYPCAEGDPYYPVPRPENNELYQKYKALADATPGVHFAGRLATYRYYN
                     MDQVVAQALTLYAKLSGVHLLKRAKPSEERAHGRDAVPTTTRPASSVA"
BASE COUNT          462 a         1040 c         1361 g          528 t
ORIGIN      
        1 cggagggctc cccggcgtcg gtggagctgt ctctggggtc ggtcgggtcc tggcggtggt
       61 cgtccggggt agtgggcgag ttcatggggg gctgagctcg gcgacggcgt cgatcggtcg
      121 gggcatcgct atgacggcgc cactttgcag gaccttgcgc agcaaggcgg cgcgctcttc
      181 actgtgatcg gcatcggtgt ggtagcggcg ggcgagctcg gcgaagccgg agctggcggt
      241 gcgctcggcc atgcgacggc acagggcggc gttctcctcc agcgcgcgca gggcggtcca
      301 cagggccatc tcgaggttgt gctcctgctc cgcggcgagg atctccgcgg tgtaggcgtg
      361 gccgacgtgg cagcgatagc gcagcagctt gccggtgtca ctggtctccc acagggcgcc
      421 gccgcactcg gggcaggtgt agggggcagg cgtgccgggg ggcgtgtgta tttgcaggct
      481 gtcgccggcc tcggcgatgt cgggggtctc gtcgggcaag gggggcctcc tggagggggc
      541 ctcgccgacg aggtctatca gcgctgcggg gatgtcgacc gctcgcagga cgcggcggac
      601 ctcgacgttc tgaatggcgc tgagcggcat gctcgggatc agcgcgtctt gcgggtcctg
      661 ggcgagcgcg acgccgccgg actcgacgat ctgcgccagg ccgtgggtgc cgtcgttgag
      721 gccgcccgag agcacgacgc cgatcgtccg cgcgccgtgg gtgtgcgcgg cggtgcggaa
      781 catcgggtcg acggccgggc gaaagccgtt ctccttcggg ccgcggacga cgcggacata
      841 gccccgcttg agcagcaggt ggtgatcggg cggcgcgacg tagacgcggc cgggctggat
      901 cggctcgtgg tcgcgggcga acgcgacggg tagcgcgctg gagcgggcga ggatctgcgg
      961 cagcagcccc gggttgtcgg gcgaggtgtg gaccacgatg aagatcgccg cccggaagct
     1021 gcccgggagc ccgcgcaaca cttcgctcag ggcggcgaga ccaccggccg aagctcccat
     1081 gacgatgata tcgcgatgca ctgccggatc ttgttgctgc aatgaaaccc ggcccgagac
     1141 tgccacccgt ccgggtgcgc cgcaaggcgg taaacgctgg tcctgcggtc cgtcggccga
     1201 ggagcgcgag caccagcgtg gcggtgggac ccggcgagcg aggcctcgcc gggctcacgc
     1261 cggttcggcg aggcggggcc ggtcgacgcg ccgcacgcgc ggccggggag cgacgcgtac
     1321 cggcggataa acgaggcgct cgggccgacg ccaccagccg ggttcggcga gcgcggggag
     1381 gtccggcgtc gcaccgcggg cgagcgcgtc gatcatgcgt gcgagctcgg tcgggcgcgg
     1441 cgggccctcg cgcaggtcga aggcgccggg ctcgtagcgg ccttcgtcgc gggtgacgag
     1501 gcagtgccag tcgtaggcgc cgagcagcga ccacgcggtg acggcccgaa gatcggcgcc
     1561 ggcgaggcga gcccgctgcg cggcgtccca gatctccttg agccagcgct gctgctcgtc
     1621 ggtcgtgcag ccgaggtgcg cctcggtgat cgcaaacggt cgcccgtagc ggcgccacac
     1681 ctcggtgaac agggcctcgg gctcgatgaa gtcggcgcac acgcgcacgg cctcgacgtc
     1741 ggcgtagcgc tggtagccgt tgccgccgat cgcggcggcc ggataacggc ggacgcgctg
     1801 gtcgaggaag cggacgctgg tgacgtagtg attgacgccg agcaggtcgg gcggcggctc
     1861 gtcggcgagg gcggcgagct cgtcggggcg ggcgccgtga cgcaggaggt agccgtggag
     1921 cgggtggttg cggtcgacgc ggccgcacag caggtcgaag gacagccagc ggcgctcgtt
     1981 ctcgaagttc gcctgatagg cgagccgcgg ggtgctcagc gtcatgccga ggtcctcggt
     2041 gacgacgagc ctggcctcgg ggttgacgac gcggatcgcc cgcatcgccg cggcggtcgc
     2101 ctgacactga tgcagcaggg cgcgcacgaa ggcggcgtcg cggcgctgat gcgggtacca
     2161 gtggccgtag aggccggaga agcgggcggt cgtcagcggc tcgttgatcg gcgtgtaggc
     2221 ggagatccac gggtaacgcc gggcgacggc gccggcgtag gcggcgagcc tgcgcgggaa
     2281 ggccgggtcg agcaggtggg tgtcgaccgg gccgctgccg tgatgcacga gcccggcgat
     2341 cggcgagatg ccgagctcgc gcaggcggtt gaagcgctcg tcggtccagc gccagtcggc
     2401 ggtgtcgagg ccccgcgggg cggtgcgctc ccacagcagc gggtagcgca gggtcttcag
     2461 gccgagcgcc gcgatcgcgt cgaggtcggc gaggcggcgg tcatggccgc tgcgcgcgag
     2521 ctgacagaga taggtgtcgc cgacgcggtt gtgtgtgcac tcgacgccgc cccacagctc
     2581 gagcggcgcc tcaggcgacg gagctcgcgg gtcgggtcgt ggtcgggact gcgtcgcggc
     2641 catgggccct ctcctcgctg ggtttggcgc gcttgagcag gtggaccccg ctgagcttgg
     2701 cgtagagcgt cagggcctgg gcgaccacct gatccatgtt gtagtagcga taggtcgcga
     2761 gccggccggc gaagtggacc ccgggcgtag cgtccgcgag ggccttgtac ttctggtaca
     2821 gctcgttgtt ctcggggcgc ggcaccggat agtacgggtc gccctcggcg caggggtact
     2881 cgtagaccac gctggtcttg gtgtgctcct ggccggtgag gtacttgaac tcggtgcagc
     2941 gcgtgtacgc gtgctcgttc gggtagttga cgaccggcgc ggtctggaac acggcgcggt
     3001 cgtgggtctc gtgcttgaac tgcaacgagc gatacggcag cttgccgtag cggtactgga
     3061 agaactcgtc gatcggcccg gtgtagatca tctcccggaa cgggatcatg tcctggacct
     3121 cgcggtaaca ggtgttggtc atcaccttga tgttcggatg gtcgagcatc ttctcgaaca
     3181 tcttcgtata gccatcggcc ggcatggcct ggaaggtatc cgtgaaatag cggtcatcag
     3241 tgttggtgcg cgtggggacg cgcgacgtca ccgacttgtc gagctgcgaa gggtcgcggc
     3301 cccattgctt gcgggtgtag ccctggaaaa acttctcgta gagctcgcgc cccaccgcgg
     3361 agacgaccac gtcctcggcg gtcttgatct c
//
LOCUS       DASWNJ010000768         2488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_579519, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000768 DASWNJ010000000
VERSION     DASWNJ010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579519"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VGB85_12860"
     CDS             <1..876
                     /locus_tag="VGB85_12860"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEY0134974.1"
                     /translation="MRGWMNEGKSYIAPVAIGEVMRAGGVGVVTESRHPGFAAGDHVA
                     GIFGIQEHCLVDLREGSRASLQKIDLRAATLSQWLNPLGIPGLTAYFGLLDVGQAKAG
                     ETVVVSGAAGAVGQTVGQVAKIHGCRVVGIAGGAAKCEFVVSELGFDACIDYKAGDVA
                     GGLKQHCPGGVDVYFDNVGGPILDTVLTRINRRARIVVCGAVSQYNNTTPIKGPSNYL
                     MLLIQRARMEGMVVFDYADRYPEAITAIAGYMQRGQLRSREDIVDGIERFPEALNRLF
                     SGENFGKLVLRVAGE"
     gene            complement(886..1647)
                     /locus_tag="VGB85_12865"
     CDS             complement(886..1647)
                     /locus_tag="VGB85_12865"
                     /inference="COORDINATES: protein motif:HMM:NF004818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0134975.1"
                     /translation="MTRTFADRVVLVTGGAAGIGRATALAFARAGARLAIVDIAEEAG
                     REVVEAITADGGEALFIRADVAVAADVEAMVARTVAAYGKLDCAFNNAGIEEEHMRLA
                     DCSEATFDRIMAINVKGVWLALKYELAAMLRGGGGAIVNTASVAGLVAAPKMAAYSAS
                     KHAVLGLTKTAAIEYARRNIRVNAVCPGVIRTPMYERALLTDPKGTAQLAQVHPIGRV
                     GEVDEVAAVVLWLCSDAASFVTGHAHTVDGGLTAV"
     gene            1752..2444
                     /gene="def"
                     /locus_tag="VGB85_12870"
     CDS             1752..2444
                     /gene="def"
                     /locus_tag="VGB85_12870"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY0134976.1"
                     /translation="MASSRLAVTVAALVLAGAGCAPAPTMSTPPAPAPAPTHPIVQVG
                     APVLRGRAAEVPPEQIASDEIQQLITRMIAAMREAPGVGLAAPQLGVPLRILVLEDRP
                     ELMTRLGPEELRERERVEVPLRVFINPTLQSVGSETRTFFEGCLSVQGYTALVERSAE
                     VEVRGLDERGQPHTMHVRGWPARILQHEVDHLDGTLYIDRMLSRSFSTIDQAKALYGG
                     LPIAEIRRRLGL"
BASE COUNT          355 a          917 c          850 g          366 t
ORIGIN      
        1 atgcgcgggt ggatgaacga gggcaagagt tacatcgccc cggtcgccat cggcgaggtg
       61 atgcgcgccg gtggtgtcgg cgtggtgacg gagtcgcggc acccgggctt cgccgccggt
      121 gatcacgtcg ccggcatctt cgggatccag gagcactgcc tcgtcgacct gcgcgagggc
      181 tcgcgcgcga gcttgcagaa gatcgacctc cgcgcggcga cgctgtcaca gtggctcaat
      241 ccgctcggca tccccggcct gaccgcctac ttcggcctgc tcgacgtcgg ccaggcgaag
      301 gccggcgaga ccgtcgtcgt ctcgggcgcc gccggggcgg tcggccagac cgtcggccag
      361 gtggcgaaga tccacggctg ccgcgtggtc ggcatcgccg gcggcgcggc caagtgcgag
      421 ttcgtggtca gcgagctcgg attcgacgcc tgcatcgact acaaggccgg cgacgtcgcc
      481 ggcggtctca agcagcactg ccccggcggc gtcgacgtgt attttgataa cgtcggcggc
      541 ccgatcctcg acaccgtgct gacgcgcatc aatcggcggg cgcgcatcgt cgtctgcggc
      601 gcggtctcgc agtacaataa tacgaccccg atcaagggcc ccagcaatta tctcatgctg
      661 ctgatccagc gcgcgcgcat ggagggcatg gtcgtgttcg actacgccga tcgctacccc
      721 gaggcgatca cggcgatcgc cggatacatg cagcgcgggc agctgcgctc gcgcgaggac
      781 atcgtcgatg gtatcgagcg gtttccggag gcgctcaacc ggctgtttag cggcgagaac
      841 ttcggcaagc tggtgctccg cgtcgccggc gagtagccgc cggcatcaca ccgccgtcag
      901 cccgccgtcg accgtgtgcg cgtgcccggt gacgaacgag gccgcgtccg agcacagcca
      961 cagtaccacc gccgcgacct cgtcgacctc accgacgcgg ccgatcgggt ggacctgcgc
     1021 gagctgggcg gtgcccttcg ggtcggtcag cagcgcccgc tcgtacatcg gcgtgcggat
     1081 gacgccgggg cacaccgcgt tgacgcggat gttcctgcgc gcgtactcga tcgcggccgt
     1141 ctttgtcagg ccgagcacgg cgtgcttgct cgcgctgtag gccgccatct tcggcgcggc
     1201 gaccagcccg gcgaccgagg cggtgttgac gatcgcaccg cccccgccgc gcagcatcgc
     1261 cgccagctcg tacttgaggg ccagccacac gcccttgacg ttgatcgcca tgatgcgatc
     1321 aaaggtcgcc tcggagcagt cggctaggcg catgtgctcc tcctcgatgc cggcgttgtt
     1381 gaaggcgcag tcgagcttgc cgtaggccgc gacggtccgg gcgaccatcg cctcgacgtc
     1441 ggccgcgacc gcgacgtcgg cgcggatgaa cagggcctcg ccgccgtccg ccgtgatcgc
     1501 ctcgacgacc tcgcgcccgg cctcctcggc gatgtcgacg atcgccagcc gcgcgccggc
     1561 tcgcgcgaac gccagcgccg tcgcgcggcc gatccccgcc gcgccgccgg tcaccagcac
     1621 caccctgtcc gcgaatgtcc tggtcatgtc gcgcgagcat acacgcccgc ggccggcggc
     1681 cgtcaggatg ccctgacggg ccgccacaaa actggcgctg gtgaggcgcg gtcgtgctac
     1741 ccggagggcc catggcatcc tctcgcctcg ccgtgaccgt cgccgcgctc gtgctcgcgg
     1801 gcgccggctg tgcgcccgcc ccgaccatgt ccaccccgcc cgcccccgct cccgccccga
     1861 cccacccgat cgtccaggtc ggcgccccgg tgctgcgcgg ccgcgccgcg gaggtccccc
     1921 ccgaacagat cgccagcgac gagatccagc agctgatcac gcggatgatc gccgcgatgc
     1981 gtgaggcccc cggcgtcggc ctcgcggccc cgcagctcgg cgtcccgctg cgtatcctcg
     2041 tgctcgagga ccgccccgag ctgatgacga ggctcgggcc ggaggagctc cgcgagcgcg
     2101 agcgcgtcga ggttcccctg cgcgtcttca tcaatcccac gctgcagtcc gtcggcagcg
     2161 agacgcgcac cttcttcgag ggctgcctca gcgtgcaggg ttataccgcg ctggtcgagc
     2221 gcagcgcgga ggtcgaggtc cgcgggctcg acgagcgcgg ccagccgcat accatgcacg
     2281 tgcgcggctg gccggcgagg atcctgcaac acgaggtcga ccacctcgac ggcacgctct
     2341 acatcgaccg catgctgagc cgatcgttct cgacgatcga ccaggcgaag gcgctgtacg
     2401 gcggcctgcc gatcgccgag atccggcgcc gcctcggcct gtgagcggcg ccggacattt
     2461 cagcgcagca cgcgcaacaa aatcgaga
//
LOCUS       DASWNJ010000769         1688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_326562, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000769 DASWNJ010000000
VERSION     DASWNJ010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326562"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..786
                     /locus_tag="VGB85_12875"
     CDS             238..786
                     /locus_tag="VGB85_12875"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003950 - NAD+ ADP-ribosyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HEY0134977.1"
                     /translation="MARDLVRVSKFLSKHLRHDPAGLGLTLGPGGWVEVDALLAACAR
                     KGTAISRSQLAEIVAGSDKQRFAFSEDGRQIRANQGHSVEVDLQLEPASEVPAALYHG
                     TGEQTVPVILREGLRRMARHHVHLSPDVETARRVGGRHGRPVVLVVDTAAMQAAGAVF
                     YCSANGVWLTEAVAPAYLRVLA"
     gene            complement(791..1303)
                     /locus_tag="VGB85_12880"
     CDS             complement(791..1303)
                     /locus_tag="VGB85_12880"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0134978.1"
                     /translation="MSEHHRPTARISRLALGLLLSLAACAGKTDAASTKPPPPELEAV
                     YYDSEATEPAFKGKPAVAQAASELEKNPDLHLLLIGRADSHGSADGNMKLGLERAHEL
                     RQVVLEQAAGKVDASRVHVGSRGQAEPTGSNDTESGRAANRRVEFYFYYPDGTTLKSR
                     FAPPIVIEGE"
     gene            complement(1322..>1688)
                     /locus_tag="VGB85_12885"
     CDS             complement(1322..>1688)
                     /locus_tag="VGB85_12885"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEY0134979.1"
                     /translation="NVAAEMLWAQMDGKVVFKHAVGTMLGALMRACTHQKIGVEDIDL
                     FLFHQANMRINQFIAQQLGVGDEKIPHNIQRYGNTTAATIPILLAECVREGKLRPGMK
                     VAMAAFGSGFTWGAAIIDW"
BASE COUNT          230 a          525 c          665 g          268 t
ORIGIN      
        1 gtcaagggag cgggcaaata agccaaggaa agccgcgagc gtggcgcggg gccgcgttca
       61 cagccagcct ggtttgcacg cgccggacca cgggcgtcgg tgtcgagatt attgatgaaa
      121 ttgtgacggg cgcgtgaacg ggcggtggac ccccggacgt cggtgtccga gcgcgcgcgc
      181 ccgggtctga cgtcgctgtc agggtgcgcg tgtcgtcggg atcggggatc ctccggcatg
      241 gcccgcgacc tggtacgtgt cagcaagttc ctcagcaagc acctgcgcca tgacccggcg
      301 ggcctcggcc tgacgctggg gccgggcggc tgggtcgagg tcgacgcgtt gctggcggcc
      361 tgcgcccgca agggcacggc gatctcgcgg tcgcagctgg cggagatcgt cgcgggcagc
      421 gacaagcaac gcttcgcgtt cagtgaggac gggcggcaga tccgggccaa ccagggccac
      481 agcgtcgagg tcgatctcca gctcgagccg gcgagcgagg tgccggcggc gctgtaccac
      541 gggaccggcg agcagacggt gccggtgatc ctgcgcgagg gtctgcgacg gatggcgcgg
      601 caccacgtgc acctgtcgcc ggacgtcgag acggcgcggc gcgtcggcgg ccggcacgga
      661 cggccggtcg tgctggtggt cgacaccgcg gcgatgcagg ccgcgggggc ggtgttctac
      721 tgcagcgcca acggggtgtg gctgacggag gcggtcgcgc cggcgtacct gcgcgtgctc
      781 gcctgagcgg tcactcgccc tcgatcacga tcggcggggc gaagcggctc ttgagggtgg
      841 tgccgtccgg gtaataaaaa tagaactcga cgcggcggtt ggcggcccgg ccggactcgg
      901 tgtcgttgct gccggtcggc tcggcctggc cacgcgagcc gacgtggacc cggctggcgt
      961 cgaccttgcc ggcggcctgc tcgagcacga cctggcgcag ctcgtgggcg cgctcgaggc
     1021 cgagcttcat gttgccgtcg gcgctgccgt gcgagtcggc gcggccgatc agcagcaggt
     1081 gcaggtcggg gttcttctcg agctcgctgg cggcctgggc gaccgcgggc ttgcccttga
     1141 acgccggttc ggtggcctcg gagtcgtaat agacggcctc gagctcgggc ggcgggggct
     1201 tggtgctcgc ggcgtccgtc ttgccggcgc aggcggccag cgagagcagc aggccgagcg
     1261 cgaggcgcga gatccgggca gtgggacggt ggtgttcgct caaggtgctc cctgtggaaa
     1321 tctaccagtc gatgatggct gcgccccagg tgaagccgga gccgaaggcg gccatggcga
     1381 ccttcatgcc ggggcgcagc ttgccctcgc gcacgcactc ggccagcagg atcgggatcg
     1441 tggcggccgt ggtgttgccg tagcgctgga tgttgtgcgg gatcttctcg tcaccgacgc
     1501 cgagctgctg ggcgatgaac tggttgatcc gcatgttggc ctggtggaac aggaacaggt
     1561 cgatgtcctc gacgccgatc ttctggtggg tgcaggcgcg catcagggcg ccgagcatgg
     1621 tgccgacggc gtgcttgaac acgaccttgc cgtccatctg ggcccacagc atctccgccg
     1681 cgacgttg
//
LOCUS       DASWNJ010000770         1839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_262317, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000770 DASWNJ010000000
VERSION     DASWNJ010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262317"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..595
                     /gene="ruvC"
                     /locus_tag="VGB85_12890"
     CDS             83..595
                     /gene="ruvC"
                     /locus_tag="VGB85_12890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY0134980.1"
                     /translation="MRILGIDPGTRLVGYGLVDCDSGASGVRGAGFRYVECGVIRLDE
                     ADSMPQRLFSLAGTLGEIIDEYAPAVVSLESAFYGVNASSALKLGQARGAIMLVAAQH
                     GLPVAEYAPALVKRSVVGHGRATKQDVQSRIKLLCRLAREPLSDAADALALALCHAQL
                     DMSAGTGRPQ"
     gene            592..1221
                     /gene="ruvA"
                     /locus_tag="VGB85_12895"
     CDS             592..1221
                     /gene="ruvA"
                     /locus_tag="VGB85_12895"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEY0134981.1"
                     /translation="MIGWLSGVVVARDPAQGIVVVNVAGVGYEVRVSLQTLAEVGDLD
                     ELCTLWIHTYAREDQLVLYGFATPAEKRLFLLLNGVPKVGPKHALAALGGFPFNELVE
                     HLRQGRHDQLQKIPGIGKKTAEQILLSIGDKVNDLGTAGEAVTTPTTPRTDGGSAQQD
                     EARAVLVGLGFRLKEVDTALAKVVAAGWQDDDGALDDLVRRTLAQLMER"
     gene            1562..>1839
                     /locus_tag="VGB85_12900"
     CDS             1562..>1839
                     /locus_tag="VGB85_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134982.1"
                     /translation="MRGEYAGRVDASIAPDVRWLAYMAFDDIVSQVSGGLPGLASGEP
                     VFKLRVADAELRVVRFAGHEAISEPFEFRVEVAGGELDPEALVGQPALL"
BASE COUNT          264 a          615 c          659 g          301 t
ORIGIN      
        1 ctcgacgagc acgacgacgt ccagaagtgc tacctcaacg cctcgctccc cgacgaagtg
       61 ctggaggagt acggcccctg aggtgcggat cctcggcatc gaccccggga cgcggttggt
      121 tggctacgga ctcgtcgact gcgatagcgg cgcgtccggg gtccgcggcg ccggctttcg
      181 ctatgtggag tgcggcgtga tccgcctcga cgaggccgac agcatgccgc agcgcctgtt
      241 ctcgctcgcc ggcacgctcg gcgagatcat cgacgagtac gcgcccgcgg tcgtctcgct
      301 cgagtcggcg ttttacggcg tcaacgccag ctcggcgctc aagctcggcc aggcccgcgg
      361 cgcgatcatg ctggtcgccg cgcagcacgg cctgccggtc gcagagtacg ccccggcgct
      421 ggtcaagcgg tcggtggtcg gccacggccg cgcgaccaag caggacgtgc agtcgcggat
      481 caagctgctg tgccgcctcg cccgcgagcc gctcagcgac gccgccgacg cgctggcact
      541 cgcgctgtgc cacgcgcagc tcgacatgtc cgcagggaca gggaggccac aatgatcggc
      601 tggctgtccg gcgtcgtcgt cgcccgcgac ccggcgcagg ggatcgtcgt cgtcaacgtc
      661 gccggcgtcg gctacgaggt ccgcgtgtcg ctgcagacgc tggcggaggt cggggacctc
      721 gacgagctgt gcacattgtg gatccacacc tatgcgcgcg aggaccagct cgtgctgtat
      781 ggatttgcca cgccggccga gaagcggctg tttcttttat taaatggggt gcccaaggtc
      841 gggccgaagc acgcgctggc ggccctcggc gggttcccct tcaacgagct cgtcgagcac
      901 ctgcggcagg ggcggcatga ccagctgcag aaaattcccg ggatcgggaa aaagaccgcc
      961 gagcagattt tgttgtcgat cggcgacaag gtgaacgacc tcgggacggc aggtgaggcc
     1021 gtgacgacgc cgacgacgcc gcgcacggac ggcggatcgg cgcagcagga cgaggcgcgg
     1081 gccgtgttgg tcgggctcgg gtttcgcctc aaggaggtcg acactgccct cgccaaggtg
     1141 gtcgccgccg ggtggcagga tgatgacggg gcgctcgacg acctggtgcg ccgcacgctc
     1201 gcccagctga tggagcgctg acgcggagcc ccgccgggga cgcgctcgac gtttcatcgg
     1261 cgcggcgaag ctcggctgat cgatccactg tcacgatcgg cccgcgtgcg tcgactcggc
     1321 tgtgggcgtc gatatcgggg cgacctgtac accgatatcg catgacgtac tttcatcgct
     1381 gtttcggcgc atccacggcc cggccggccc gcgggtcggg ccgtccgatc cgcgcgcgag
     1441 cctgcctgcg ccgccgcgct cggggcctcg cagccccagc ttgcgatatc gcggtccgcc
     1501 gcaccgcgca tcgacaccgg gtcgtgttcg tccgcgctcg gacgatcacg cggaatcgtt
     1561 catgcgcggc gaatatgccg gacgagttga cgcttcgatc gcgccggacg tacgctggct
     1621 cgcttatatg gcattcgacg acatcgtctc tcaagtatcg ggggggttgc cgggcctcgc
     1681 gtccggcgag ccggtgttca agctgcgtgt cgccgatgcc gaattgcgcg tggtccgatt
     1741 cgccgggcac gaggcgatct cggagccgtt cgaatttcgc gtcgaggtcg cgggcggcga
     1801 gctcgatccg gaggcgctgg tcgggcagcc ggcgctcct
//
LOCUS       DASWNJ010000771         5338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_262319, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000771 DASWNJ010000000
VERSION     DASWNJ010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262319"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VGB85_12905"
     CDS             <1..258
                     /locus_tag="VGB85_12905"
                     /inference="COORDINATES: protein motif:HMM:NF025619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4351 domain-containing protein"
                     /protein_id="HEY0134983.1"
                     /translation="ITADNGEEVMQTIAQMLEARGMERGLQQGRQEGRRELVQQQLQR
                     RFGELPAAVIQRLAAAEADELERWADRLLFAGNLDEVFREP"
     gene            complement(294..869)
                     /gene="def"
                     /locus_tag="VGB85_12910"
     CDS             complement(294..869)
                     /gene="def"
                     /locus_tag="VGB85_12910"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY0134984.1"
                     /translation="MAILKVAQIGHPVLRQQARPVTREELRTPAFQAFIDSMIDTMHD
                     AAGAGLAANQVHEPVQVCVIEVKDNPRYPYKPNIPLTVLVNPVLTPLSETKFENFEGC
                     LSVPDLRGIVRRYVEIRVQAWDRHGEPIDAVIRGITAGTYQHECDHLAGSLFIDHVED
                     PRTLCTWKEFSRQHEARFREHVKEIVEHYGS"
     gene            complement(968..2641)
                     /locus_tag="VGB85_12915"
     CDS             complement(968..2641)
                     /locus_tag="VGB85_12915"
                     /inference="COORDINATES: protein motif:HMM:NF041620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 family protein"
                     /protein_id="HEY0134985.1"
                     /translation="MLLTKIWAAILACLATAFLAGMFLLSFGTAGGFTEADRAALQAT
                     TEAGVAALAAEIQASPVSRAPAMLQDPSLKLALFPPPVPPKDGTLMDVPSALSNVANT
                     GLLQDHPQMTVALFDSRGAVIASHGAETEKIVEVSRLPAYANVKQDQEAQFSATLGGS
                     LEVVKVSEALTNGTRLVAIHPLDLGATSFFRQILGTRSPAALIRGNAPLENGTINAQN
                     IGDAFTKFATEHGKEAPPVGASVVHRVGEGTGARIGSVGRVPGPAGTGDDGTVLVVLS
                     DKTAAAGQKELLPALREATNNGMTGEHWLALVFVLALSLALALYLPNMEITGPLQRLT
                     AEFNAMRNGTQQQLFYDTYGNGPVGELAKAAHQYHEALRSSLQANEGVADEGDDKNRV
                     SRRMQAVNQTRSNRRVPPGRTGAAQVPDAPIEEAPTTAHPSVPVPAAGAQGGRGPGFF
                     TMPATKPLRSRETVIGNLMTLDNDPSQPEPAKDDAVREAYYQEVFEEFVKFKVENNES
                     IDNFTYDKFATKLRANTAELMKRPDVKDVQFSVYIKDGKVALKARVVRG"
     gene            3153..4676
                     /locus_tag="VGB85_12920"
     CDS             3153..4676
                     /locus_tag="VGB85_12920"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEY0134986.1"
                     /translation="MLWVSAGDLSSPAAPRWPGDGQGRSPGPSFHAEQLWLASGAPDG
                     YWAAVARLRDAGEAAARGEPARGFVLAAVLGLPAALPPTTRSELRRAGLGHLVAVSGM
                     NVAVAALLLRAPLLRLGLLLGGSPALGSALAWLPVAAYVGLTGAAAPAVRAAVMFTLV
                     QLGVLCGRPAHGMTTLALAAAGLLAWSPAWAGDPGFHLSMVAMAVLLRPVAPGEAPPG
                     LLAQSWQITWATCPVGLLHFGHAAVWGVLANLVAVPVFTLWVAPLGTIGCLLWPWIGD
                     RVLAPAAWGGQLILDLAAVLARAPEIPIDGLLVAAAVMLALWWLWPRCRRGLPGPWVC
                     AATIAAVIVARPDPDVTPPAWFAAGGGREHALVVPATSPGLACIRDPNLFPEAWPSLL
                     RALGYRGVAGLAVKKGVEPPHLVELREQLMREGMWAPGECSFAEAALVEAAVVRLALR
                     ACLARTGQKTAAVRAGPDCHFGGAWGSLAEDGPPARVLRALGTAWQDLTERIDSRPP"
     gene            4673..5146
                     /locus_tag="VGB85_12925"
     CDS             4673..5146
                     /locus_tag="VGB85_12925"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0134987.1"
                     /translation="MTDLTIAVREQTGIDAGRVHHLRLGRHVIGRDPSASIQLRSTDV
                     SRQHAILEVSPEAVTVTDLGSKNGVLLKRKHGKTAITGPTLLADGAIVEVGGVDLLVA
                     HPGAQVDRALARVGEATITRIEGSGAAAGAGPGPLVPLLLTATFAAVVAALLWLG"
     gene            5158..>5338
                     /locus_tag="VGB85_12930"
     CDS             5158..>5338
                     /locus_tag="VGB85_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134988.1"
                     /translation="MRKLPRVDAILAEPVLVEHPASHAVKKRAVQQALASRRAALRAG
                     DPGDMSEETADEVGVV"
BASE COUNT          642 a         1690 c         2140 g          866 t
ORIGIN      
        1 atcaccgccg acaacggaga agaagtcatg cagaccatcg cgcagatgct ggaggcgaga
       61 ggaatggagc gggggttgca gcaggggcgc caggagggcc ggcgggagct ggtgcagcag
      121 cagctgcagc ggcgcttcgg cgagttgccg gcggcggtca tccagcgcct cgccgcggcc
      181 gaggccgacg agctcgagcg ctgggccgac cgtttgctgt tcgccggcaa cctcgacgag
      241 gtcttccgcg agccctgagc cgggtctcgc gctgcgcggc gtcgcggcgg gcctcaggac
      301 ccgtagtgct cgacgatctc cttcacgtgc tcgcggaagc gggcctcgtg ctggcgggag
      361 aactctttcc aggtgcacag ggtgcgcggg tcctcgacgt ggtcgatgaa gagggagccg
      421 gcgaggtggt cgcactcgtg ctggtaggtg ccggccgtga tgccgcggat gacggcgtcg
      481 atcggctcgc cgtgacggtc ccacgcctgc acgcggatct cgacgtagcg gcggacgatg
      541 ccgcgcaggt cgggcaccga caggcagccc tcgaagttct cgaacttggt ctccgacagc
      601 ggcgtgagca cggggttcac gagcaccgtc agcgggatgt tcggcttgta cgggtagcgc
      661 gggttgtcct tgacctcgat gacgcacacc tggaccggct cgtggacctg gttggccgcg
      721 aggccggcgc cggcggcgtc gtgcatggtg tcgatcatgc tgtcgatgaa cgcctggaag
      781 gccggcgtgc gcagctcctc gcgggtgacg gggcgggcct gctggcgcag gacggggtga
      841 ccgatctggg cgaccttgag gatggccatg gtgctctctt taaaaatcga aaacggccgg
      901 accttgcggg tccggccgtc actttggggc cgctctcggg cccggtcaac cgggcgtcac
      961 gaagtcgtca gccgcggacg acgcgggcct tgagcgcgac cttgccgtcc ttgatgtaga
     1021 ccgagaactg cacgtccttg acgtcggggc gcttcatcag ctcggccgtg ttggcgcgca
     1081 gcttggtggc gaacttgtcg taggtgaagt tgtcgatgct ctcgttgttc tcgaccttga
     1141 acttcacgaa ctcctcgaag acctcctggt agtaggcctc gcgcaccgcg tcgtccttgg
     1201 ccggctcggg ctgcgacggg tcgttgtcca gcgtcatcag gttgccgatg acggtctcgc
     1261 gggagcgcag cggcttggtc gccggcatcg tgaagaagcc ggggccgcgg ccgccttggg
     1321 cgccagcggc ggggaccggc acggagggat gcgcggtggt cggcgcctct tcgatcggcg
     1381 cgtcggggac ctgcgcagcg ccggtgcggc cgggcggcac gcgacggttg ctgcgggtct
     1441 ggttgacggc ctgcatgcgc cgcgagacgc ggttcttgtc gtcgccctcg tcggcgacgc
     1501 cctcgttggc ctgcaggctc gagcgcagcg cctcgtggta ctggtgcgcg gccttggcga
     1561 gctcaccgac cggaccgttg ccataggtgt cgtagaacag ctgctgctgg gtgccgttgc
     1621 gcatcgcgtt gaactcggcg gtcaggcgct gcagcgggcc ggtgatctcc atgttcggca
     1681 ggtagagcgc gagcgcgagg ctgagggcca gcacgaacac gagcgcgagc cagtgctcgc
     1741 cggtcatgcc gttgttggtc gcctcgcgca gcgcgggcag cagctccttc tggccggcgg
     1801 cggcggtctt gtcggagagc acgacgagca cggtgccgtc gtcgccggtg ccggcggggc
     1861 cgggcacgcg accgacggag ccgatgcggg cgccggtgcc ctcgccgacg cggtgcacga
     1921 cggaagcgcc gaccgggggc gcctccttgc cgtgctccgt ggcgaacttg gtgaacgcgt
     1981 cgccgatgtt ctgcgcgttg atcgtgccgt tctccagcgg cgcgttgccg cggatcagcg
     2041 cggcggggct acgcgtgccg aggatctggc ggaagaacga cgtggcgccg aggtcgagcg
     2101 gatggatcgc gaccaggcgc gtgccgttgg tcagcgcctc cgagaccttg acgacctcga
     2161 gcgagccgcc gagcgtcgcc gagaactgcg cctcttgatc ctgcttgacg ttggcgtacg
     2221 cgggcagccg cgagacctcg acgatcttct cggtctcggc gccatgcgag gcgatcaccg
     2281 cgccgcggct gtcgaacagc gcgacggtca tctgcgggtg gtcctgcagc aggccggtgt
     2341 tggcgacgtt gctgagggcc gagggtacgt ccatcaaggt gccgtccttc ggcggcaccg
     2401 gtggtgggaa caaggccagc ttcagcgagg ggtcttgcag catcgccggc gcgcgagaca
     2461 caggcgatgc ctggatctct gccgcgagcg cggcgacgcc ggcttcggtc gtcgcctgca
     2521 gcgccgcacg atcggcctcg gtgaacccac ccgcagtgcc aaaggacaac aggaacatcc
     2581 ccgcgaggaa ggccgtcgcg aggcatgcaa ggattgcagc ccagatcttc gtcagcagca
     2641 cgattgggcc tagtgtatgg ccttgcggcc ggcgctggca atttcgtgat gtgctccgcg
     2701 tgctctgggc gggatcaccc actcgaagcg tgctgtcgcg caacgggtcg gggacgcctg
     2761 ccgaatcccg gggcgtgacg accctggctc tggcgctcat ttttgccgca ggtacggcgc
     2821 tcgggctcgc gctcgggccc tgtgcctgcg cgctggcgat cgtcggcgcc ggggccgtgc
     2881 tggccgccgt ggcggtgctc cccggggtgg atgggcggtt ccgagcatgt ctcgccgccc
     2941 tcgcgctcgg tctgtgtgga cgggtccatg gcgggttggc ggcacgagcg caggggccct
     3001 ttccggcggg cccggggctc gtaaagctgg aggtaagcgg ggccagcatg ccgaggggtg
     3061 ctcgctgcga ggtggacgtt cggccggtgg ggagcaccct gcgatggcgg ttgcaggtcg
     3121 atcccgcgcg ctgtccgctg tcgcaagggc agttgctgtg ggtttcggcc ggagatctct
     3181 cctcgcccgc ggccccgcgc tggccgggtg atggccaggg tcggtcgccc gggccgagct
     3241 ttcatgcgga gcagctgtgg ctcgcgagtg gcgcgcccga cggctactgg gcggcggtcg
     3301 ctcgtctgcg tgacgccggc gaggcggcgg cgcgtggtga accggcccgc ggcttcgtgc
     3361 tcgcggcggt gctcggcttg ccggccgcgt tgccgccgac gacgcgcagc gagctacgca
     3421 gggccgggct cggacatctg gtggcggtca gcgggatgaa cgtggcggtc gcggccctgc
     3481 tgctgcgggc gccgctgctg cgcctcggcc tgctgctcgg aggatctccg gcgctgggca
     3541 gcgcgctggc atggctgccg gtggcggcgt acgtcggcct gacgggggcc gcggcgccgg
     3601 cagtccgcgc ggcggtgatg ttcacgctcg tgcagctcgg tgtgctgtgc ggacgcccgg
     3661 cgcacggcat gacgacgctg gcgctggcgg ccgcgggtct gctggcgtgg tctccggcgt
     3721 gggcaggaga ccccggcttt catctctcga tggtggcgat ggcggtgttg ttgcggccgg
     3781 tggcgcccgg tgaggcgccg ccggggttgc tggcgcagag ctggcagatc acgtgggcga
     3841 cctgcccggt cgggctgctg cactttgggc acgcggcggt gtggggcgtg ctcgccaacc
     3901 tcgtcgcggt gccggtgttc acgctgtggg tcgcgccgct cgggacgatc ggctgcctgc
     3961 tgtggccgtg gatcggtgac cgggtgctcg cgcccgcggc gtggggcggt cagctgatcc
     4021 tcgatctcgc ggcggtgctg gcccgcgcgc cggagatccc gatcgacggg ttgctggtcg
     4081 cggcggcggt gatgctggcg ctgtggtggc tctggccgcg ctgccgtcga gggctgccag
     4141 ggccgtgggt gtgcgcggcg acgatcgcgg cggtgatcgt cgcgcggccg gatccggacg
     4201 tgacgccgcc ggcgtggttc gcggccggtg gcggccgcga gcacgcgctc gtggtgcccg
     4261 cgacgagccc gggactggcg tgcatccgtg acccgaattt gttccccgag gcgtggccgt
     4321 cgctgctgcg tgcgctcggg tatcgcgggg tcgcggggct cgcggtgaaa aaaggggtcg
     4381 agccgccgca cctggtcgaa ctgcgcgagc agctgatgcg cgaggggatg tgggccccgg
     4441 gcgagtgctc gttcgcggag gcggcgctcg tggaggcggc ggtggtgcgg ctggcgctgc
     4501 gcgcctgcct ggcgcgcacg ggccagaaga ccgcggcggt gcgagccggg ccggactgcc
     4561 acttcggtgg ggcgtggggg tcgctcgccg aggatgggcc gccggcgcgg gtgttgcggg
     4621 cgctggggac cgcctggcaa gacctgaccg aaaggataga ctcgcgcccg ccatgacgga
     4681 cctgacgatc gcggtgcgcg agcagacggg gatcgacgcc ggccgtgtcc atcacctgcg
     4741 cctcggcagg cacgtgatcg ggcgcgatcc ctcggcgtcg atccagctgc gtagcaccga
     4801 cgtgtcgcgc cagcacgcga tcctcgaggt ctcgccggag gcggtgaccg tcaccgacct
     4861 cggcagcaag aacggggtgc tgctcaagcg caagcacggc aagacggcga tcacggggcc
     4921 gacgctgctc gccgatgggg cgatcgtcga ggtcggtggg gtcgatctgc tggtcgcgca
     4981 tcccggcgcg caggtcgacc gcgcgctggc ccgcgtcggc gaggctacga tcacgcgcat
     5041 cgagggatcc ggggcggcgg cgggcgcggg gcccgggccg ctggtgccgc tgttgttgac
     5101 ggcgacgttc gcggcggtcg tcgcggcgtt gctgtggctg gggtgacgat gaggacgatg
     5161 aggaagttgc cgcgcgtcga tgcgatcctc gcggagccgg tgctcgtgga gcatccggcc
     5221 agccacgcgg tcaagaagcg cgcggtgcag caggcgctgg cgagccggcg cgcggcgctg
     5281 cgggcgggtg accccgggga catgtccgaa gagacagcag acgaggtcgg ggtggtcg
//
LOCUS       DASWNJ010000772         3427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_507435, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000772 DASWNJ010000000
VERSION     DASWNJ010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507435"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..2163)
                     /locus_tag="VGB85_12935"
     CDS             complement(94..2163)
                     /locus_tag="VGB85_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEY0134989.1"
                     /translation="MSLVVFEGVSLGFGQKTIVADLNLRLGASDRIGLIGPNGSGKTS
                     LLRMLAGQQSFDKGSLRFARGVRVGYLAQDLVVEGGQTLSNFVVNSVPGRAEVDQLLA
                     DAEADLAAAQAAADANPGDAEHERLMEVAERLAEVHERIAHFDAHFTEHEALRILAGL
                     GFLATDATRDLGEFSGGWKMRAVLAALLFQQPDLLLLDEPTNHLDMPSVAWFAAYLKR
                     YQRAFVLVSHDREFLNEQIGRVVSFEPEGVRTFTGNYEGYLKQRVEEEVLLENRAKNL
                     AREREKAEQFIERFRAQASKASGVQSRVKALEKMEEVKTFVRRQVMRFEFPPTDRAGD
                     TVLKVDGLRKAYGDHVVLDKVELTVRRGEKIGIIGVNGAGKTTLLRAIAGEVPIEAGS
                     INFGNKVKVGYYAQHHADTLRPDRTLFEEVSAQAPSAGQTRVRSVLGAFLFSGDDVDK
                     KIRVLSGGERARVALARLLISPGNVMLMDEPTNHLDLDSSESLAESLRSYDGTLLFVS
                     HNRSFVRKLATRIWDVSGGKVETYGGTLDEYMDSMRRRRDGEPEVRGEKALQAARPTH
                     VTPAKQPQKGGQKPAVVAAPEPARGKQDRRREAELRNERNKVLGPLKKKVAEMETRIA
                     ALEVEQKQRSAALADPATYADDKLRAKLLSGYQVDADKLEELTRRWEISVAELEQLEA
                     ELKSEAS"
     gene            2248..2727
                     /locus_tag="VGB85_12940"
     CDS             2248..2727
                     /locus_tag="VGB85_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134990.1"
                     /translation="MTEALDPETLERLRRGIPLRLDRTGQFHLGDEPVIHPRVRAAFL
                     AGLDHNDAGEPTLHVGNQWCYLTVDDCPLRATGILPDPDGALTMRLDDGRTVPLDPTT
                     VRVDDEGLRCLAPSQPTSRPIPVRLGNTAAMDLSAFIEGDGDTATLVVGSQRIALRR"
     gene            3040..>3427
                     /locus_tag="VGB85_12945"
     CDS             3040..>3427
                     /locus_tag="VGB85_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134991.1"
                     /translation="MAALCLASACGDDGNATTGFTTAPTTVATNTSSPATDPNATSAP
                     NDATTDTPTTGLSTTTTDATDAMDTTVDPTAHTTTGMDTTGSDPMCGDGMIDPGETCD
                     DGPANGPGQPCNAMCAANACGDGDQGP"
BASE COUNT          517 a         1358 c         1038 g          514 t
ORIGIN      
        1 gagcggaccg accggaccga gcggaccgac cggaccgagc ggaccgaccg ggccaaggcg
       61 ggccgccggc gtgcccctcg gctcgccggc gcgctagctc gcctcgctct tgagctccgc
      121 ttcgagctgc tcgagctccg ccacgctgat ctcccagcgc cgcgtcagct cctcgagctt
      181 gtccgcgtcg acctgatacc cgctgagcag cttggcccgc agtttgtcgt ccgcgtaggt
      241 cgccgggtcg gccagcgccg cgctgcgctg cttctgctcc acctcgagcg ccgcgatccg
      301 cgtctccatc tcggcgacct ttttcttcaa cggcccgagc accttgttgc gctcgttgcg
      361 gagctccgcc tcgcggcgac ggtcctgctt gccgcgcgcc ggctccggcg cggcgaccac
      421 ggccggcttc tgcccgccct tttgcggctg cttcgccggc gtcacgtgcg tcggccgcgc
      481 ggcttgcagc gccttctcgc cgcggacctc cggctcaccg tcacgccggc gccgcatgct
      541 gtccatgtac tcgtcgagcg tgccgccgta ggtctcgacc ttgccgccgc tgacgtccca
      601 gatcctcgtc gccagcttgc gcacgaagct gcggttgtgg ctgacgaaca gcagcgtgcc
      661 gtcgtagctg cgcagcgact ccgccaggct ctcgctcgag tcgaggtcga ggtggttggt
      721 cggctcgtcc atcagcatca cgttgccggg gctgatcagc agccgcgcca gcgcgacgcg
      781 ggcccgctcg ccgccggaca gcacgcggat cttcttgtcg acgtcgtccc cgctgaacaa
      841 aaacgccccg agcacgctgc gcacgcgcgt ttgtcccgcg ctcggcgcct gcgccgaaac
      901 ctcttcaaag agagtcctgt cgggtcgcag cgtgtccgcg tggtgctgcg cgtagtagcc
      961 gaccttgacc ttgttgccga agttgatcga cccggcctcg atcgggacct cgccggcgat
     1021 cgcccgcagc agcgtcgtct tgccggcccc gttgacgccg atgatcccga tcttctcgcc
     1081 gcgacggacc gtcagctcga ccttgtcgag caccacgtgg tcaccgtagg ccttgcgcag
     1141 cccgtcgacc ttgagcaccg tgtcgcccgc gcggtccgtc ggcgggaact cgaagcgcat
     1201 cacctggcgg cggacgaagg tcttgacctc ctccatcttc tcgagcgcct tgacccggct
     1261 ctgcacgccc gacgccttgc tcgcctgggc ccggaagcgc tcgatgaact gctcggcctt
     1321 ctcgcgctcg cgcgcgaggt tcttggcccg gttctcgagc agcacctcct cctcgacgcg
     1381 ctgcttgagg taaccctcgt agttgccggt gaacgtccgc accccctcgg gctcgaagct
     1441 gacgacgcga ccgatctgct cgttgaggaa ctcgcggtcg tgcgacacca gcacgaacgc
     1501 ccgctggtag cgcttgaggt acgccgcgaa ccacgctacc gacggcatgt cgaggtggtt
     1561 ggtcggctca tccaacaaca gcaggtccgg ctgctgaaac agcagcgccg ccagcaccgc
     1621 gcgcatcttc cagccgcccg agaactcgcc gaggtcccgc gtcgcgtcgg tcgccaggaa
     1681 cccgaggccc gcgaggatcc gcagcgcctc gtgctcggtg aagtgcgcgt cgaagtgggc
     1741 gatccgctcg tgcacctcgg ccagccgctc cgcgacctcc atcaagcgct cgtgctccgc
     1801 gtccccgggg ttggcgtcgg ccgccgcctg cgcagccgcg aggtcggcct ccgcgtccgc
     1861 gagcagctgg tcgacctccg cccgccccgg cacgctgttg accacgaagt tgctcagcgt
     1921 ctgcccgccc tcgaccacca ggtcctgcgc gaggtacccg acccgcaccc cgcgagcgaa
     1981 gcgcagcgac cctttgtcaa agctctgctg cccggccagc atccgcagca gcgaggtctt
     2041 gcccgacccg ttgggcccga tcaggccgat gcgatcgctc gcgccgagcc gcaggttgag
     2101 atcggcgacg atcgtctttt gcccgaagcc gagcgacact ccctcgaaga cgaccagact
     2161 cacgggcgcg ggttataaca gcgtgtcgcc ccggcacaag ggcccccgtc gcgcgcgcgg
     2221 gccgcaaacc cggcttacac tgcgaccatg accgaggccc tcgaccccga aaccctcgaa
     2281 cgcctgcggc gcgggatccc gctccgcctc gaccgcaccg gccagttcca cctgggtgac
     2341 gagcccgtca tccacccccg cgtccgcgcc gccttcctcg ccggactcga ccacaacgac
     2401 gccggcgagc cgaccctcca cgtcggcaac cagtggtgtt acctcacggt cgacgactgc
     2461 ccgctgcgcg ccaccggcat cctccccgac cccgacggcg ccctgaccat gcgcctcgac
     2521 gacggccgca ccgtccccct cgatccgacc accgtccgcg tcgacgacga gggcctgcgc
     2581 tgcctcgccc cctcccagcc gacctcgcgc ccgatccccg tccgcctcgg caacaccgcc
     2641 gcgatggatc tcagcgcctt catcgagggc gacggcgaca ccgccaccct cgtcgtcggc
     2701 tcccagcgca tcgccctgcg ccgctgatcc tcgcctccgt cgcccgcaca cgagcgcgcg
     2761 cttgcgaacc cacccggagc accgcagcaa actacctaat ccatccacgc cacgagatac
     2821 caacaaagcc actccgcgac gcagacatca gcaaataatt aactaccagc caatcgctgc
     2881 caaccggtcc gccagcgaag gcccaccatt gatgaagtct tcacaggggc tgcgtcgcat
     2941 atatcgacag cacaccgacg ccgggtccat tctgaaaaaa tggaaatgca acgtcttgtg
     3001 aacacgcggt gggtctcggg tctcggttcc ttgcggctga tggccgcgct gtgtctggcg
     3061 tcggcgtgcg gcgacgacgg caacgccacg accggcttca cgacggcgcc gacgacggtg
     3121 gcgacgaaca catcgtcgcc ggccaccgac ccgaacgcga cgagcgcccc gaacgacgcg
     3181 acgaccgaca ccccgacgac cggcctctcg acgaccacca ccgacgcgac cgacgcgatg
     3241 gacaccacgg tcgacccgac cgcgcacacg accaccggta tggacacgac cggcagcgac
     3301 ccgatgtgcg gcgacggcat gatcgacccg ggcgagacct gcgacgacgg gcccgccaac
     3361 ggccccggcc agccctgcaa cgcgatgtgt gccgccaacg cctgcggcga cggcgaccag
     3421 ggtcccg
//
LOCUS       DASWNJ010000773         3314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_439723, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000773 DASWNJ010000000
VERSION     DASWNJ010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439723"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..2265)
                     /locus_tag="VGB85_12950"
     CDS             complement(22..2265)
                     /locus_tag="VGB85_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134992.1"
                     /translation="MRRSYDLTRSSYLFLGSALALTLAACGDDGGQATETESGTSSTG
                     DATDTDVTPTTQPTEPNPTEPNTTEPLTTSDTSTTDPLTTDPTTDTTTDGTTTDATTT
                     GDTTTGDTTTTDTTTGDTGDAEVAGVWASANPTGLMTDALVGLTPTLDMQTAKVAVLG
                     DLISVQSVGIDADGDAVISFDEPGGTGGIIIDINLADNPMNSAIGLGDRVIRGPATGL
                     IAPKGVEAVGPDFLFLVADTGAMDIKAFDADDEGDVAPIFVVSDLGTSTVVWDIHYVV
                     TADVLYAAGTNGEVQVYENFSNDMGQTGPDRTIVPTMNNQKISINLHGIAVENNQLFL
                     SDVGDAMKTDDGQLFRIANASGLDGNENVTQRIQGGDLGNPVDIDVRPGPGPTDVLFV
                     AEKSNDLVLVYRENVMGNFAIGGELAVEKPESVAVAPAMNRVIVAKNPAGLDTDAVLA
                     LNAPAIGNPTLTATLDRLGSVTSVQSLVLADNGDAYVGFDGPSISGGGGVFLVPVLTN
                     AAADSETNAATAMRLWGENTGIVTPKGLLLTSLEDRLIVADTGGPDIKVFDTAALGDT
                     APLFVVDELGGGPVWDIAYDNLADRLYAAGTDGVVRVFDDFMANEGQGGPDRLITPTD
                     DQDEKISVNLHGIEYDAASDTLILSDVGDAMSATDGAIFVITAASTADDNVEVAATIR
                     GDATKLGNPVDISFDGANLYVAEKSNSSVLRYDAILGLTGNNNTAENAMIEVANPESV
                     ALAFTQP"
     gene            complement(2419..3234)
                     /locus_tag="VGB85_12955"
     CDS             complement(2419..3234)
                     /locus_tag="VGB85_12955"
                     /inference="COORDINATES: protein motif:HMM:NF039689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6209 family protein"
                     /protein_id="HEY0134993.1"
                     /translation="MSRLLPVTLPLSLVSLLLAACEATAPEIEVREGNAPPAGLQPAT
                     LSFTDNWQIVRGGGPLVAGNPVEVAYDADRLTACRGEQYGKPAWSITGYHQLEDGPVG
                     SFEAGGFSPSQGTEAPIFTPASSGDLALWFHNTSVWGCSAHDSNFGANWHAQVGGSLS
                     FKTGWTIEGFGAPRAGADLVIAYDPARLPDCRGSKYGFEAWNIRVHYRFDGGPVQVAM
                     LTEAAGNVQVPVPAVLAAPADAEVVEMWFENQSYSGCRAWDSRFGENYSFALE"
BASE COUNT          438 a         1026 c         1283 g          567 t
ORIGIN      
        1 ccgggcgacg agtcgtcgcg gtcagggctg ggtgaaggcg agggccacgg actcggggtt
       61 ggcgacctcg atcatcgcgt tctcggccgt gttgttattg ccggtgaggc cgaggatcgc
      121 gtcgtagcgc aggaccgagg agttggactt ctcggcgacg tagaggttgg cgccgtcgaa
      181 ggagatgtcg acggggttgc cgagcttggt cgcgtcgccg cggatcgtgg cggcgacctc
      241 gacgttgtcg tcggcggtcg aggcggcggt gatcacgaag atcgcgccgt ccgtcgcgga
      301 catcgcgtcg ccgacgtcgc tgaggatcag cgtgtcgctc gcggcgtcgt actcgatgcc
      361 gtggaggttg acgctgatct tctcgtcctg atcgtcggtc ggcgtgatca ggcggtcggg
      421 gccgccctgg ccctcgttgg ccatgaagtc gtcgaacacg cgcacgacgc cgtcggtgcc
      481 ggcggcgtac aggcggtcgg cgaggttgtc ataggcgatg tcccacaccg ggccgccgcc
      541 gagctcgtcg acgacgaaca gcggggcggt gtcgccgagc gcggcggtgt cgaacacctt
      601 gatgtccggg ccgccggtgt cggcgacgat caggcggtct tccaggctgg tgaggagcag
      661 gcccttgggc gtgacgatgc cggtgttctc gccccacagg cgcatggcgg tcgcggcgtt
      721 ggtctcgctg tcggccgcgg cgttggtgag gacggggacg aggaacacgc cgccgccgcc
      781 ggagatgctc gggccgtcga agccgacgta ggcgtcgccg ttgtcggcga gcacgaggct
      841 ctgcacggag gtgacgctgc cgaggcggtc gagggtggcg gtgagggtcg ggttgccgat
      901 cgcgggggcg ttgagggcga gcacggcgtc ggtgtcgagg ccggcggggt tcttggcgac
      961 gatgacgcgg ttcatcgcgg gggcgaccgc cacggactcc ggcttctcga ccgcgagctc
     1021 gccgccgata gcgaagttgc ccatgacgtt ctcgcggtag acgagcacga ggtcgttgga
     1081 cttctcggcg acgaagagga cgtcggtcgg gccggggccg gggcggacgt cgatgtcgac
     1141 cgggttgccg aggtcgccgc cctggatccg ctgggtgacg ttctcgttgc cgtcgaggcc
     1201 gctggcgttg gcgatccgga acagctggcc gtcgtcggtc ttcatcgcgt cgccgacgtc
     1261 gctcaggaac agctggttgt tctcgacggc gatgccgtgg agattgatcg agatcttctg
     1321 gttgttcatc gtcgggacga tcgtgcggtc cgggccggtc tggcccatgt cgttcgagaa
     1381 gttctcgtag acctggacct cgccgttggt gcccgcggcg tacagtacgt cggcggtgac
     1441 gacgtagtgg atgtcccaca cgaccgtcga ggtgccgagg tcggagacga cgaagatcgg
     1501 ggcgacgtca ccctcgtcgt cggcgtcgaa ggccttgatg tccatcgcgc cggtgtcggc
     1561 gacgaggaac aggaagtcgg gcccgacggc ctcgacgccc ttgggggcga tcaggccggt
     1621 cgccggccca cgaatgacgc ggtcgccgag gccgatcgcg ctgttcatcg ggttgtcggc
     1681 gaggttgatg tcgatgatga tgccgccggt cccgccgggc tcgtcgaagc tgatcacggc
     1741 gtcgccgtcg gcgtcgatgc cgacgctctg gacgctgatc aggtcgccga gcaccgcgac
     1801 cttggcggtc tgcatgtcga gcgtgggggt caggccgacc agcgcgtccg tcatcaggcc
     1861 cgtcgggttg gcgctcgccc acacgccagc gacctcggcg tcgccggtgt cgccggtggt
     1921 cgtgtcggtc gtggtggtgt cgccggtggt cgtgtcaccg gtggtcgtcg cgtccgtggt
     1981 ggttccgtcg gtggtcgtgt cggtcgtcgg gtcggtggtc agcggatcgg tcgtcgacgt
     2041 gtcgctggtc gtcagcggct cggtggtgtt gggctcggtg gggttgggct cggtcggctg
     2101 ggtcgtcggc gtgacgtcgg tgtcggtggc gtcgccggtc gagctggtcc cggactcggt
     2161 ctcggtcgcc tggcccccgt cgtcaccaca cgccgcgagg gtcagcgcga gcgcggagcc
     2221 gaggaagagg taggagcttc gggtaaggtc gtatgatcta cgcataggca tcggatccag
     2281 acatcccata cgtgacgact tccagcggag gatcaggagg ctccgcgggc ctgtgtcatc
     2341 gatcaccgcc gcaggcggcc cgagctgttg tttgcgggcg tcctccgcag cgagggcagt
     2401 ccgcaactcg cgccaggctc actcgagcgc gaagctgtag ttctcgccga agcgcgagtc
     2461 ccaggcccga caaccgctgt agctctggtt ctcgaaccac atttcgacga cctcggcgtc
     2521 tgcgggcgcg gcgagcaccg cgggcacggg cacctgcacg ttgcccgccg cctcggtcaa
     2581 catcgcgacc tgcaccgggc cgccgtcgaa gcggtagtgg acgcggatgt tccaggcctc
     2641 gaagccgtac ttgctgccgc ggcagtcggg caggcgcgcg gggtcgtagg cgatgacgag
     2701 gtccgcaccg gcgcgtgggg ccccgaagcc ctcgatcgtc cagcctgtct tgaaggacag
     2761 gctgccaccg acctgcgcgt gccagttcgc gccgaagttg gagtcgtggg ccgagcaacc
     2821 ccacacgctg gtgttgtgga accacaacgc gaggtcgccc gagctcgcgg gggtgaagat
     2881 cggcgcctcg gtgccctgcg agggcgagaa gccgccggcc tcgaagctgc cgaccgggcc
     2941 gtcctcgagc tggtggtagc cggtgatcga ccacgcgggc ttgccgtact gctcgccgcg
     3001 gcaggcggtc aggcggtcgg cgtcgtaggc gacctcgacc gggttgccgg cgacgagcgg
     3061 accgccgccg cggacgatct gccagttatc ggtgaagctc agcgtcgcgg gctgcaagcc
     3121 ggccggcggc gcgttgccct cgcgcacctc gatctccgga gcggtcgcct cgcaggcggc
     3181 cagcaagagc gagacaagcg agaggggcag ggtgaccggg agcaggcgcg acatgcaggc
     3241 ggcgttttaa caaaccgcca ctgcgccggc aatcgccggg aagaccccgg cgtggggctg
     3301 cgcgcctccc gtgt
//
LOCUS       DASWNJ010000774         1947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_119706, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000774 DASWNJ010000000
VERSION     DASWNJ010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119706"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1947)
                     /locus_tag="VGB85_12960"
     CDS             complement(<1..>1947)
                     /locus_tag="VGB85_12960"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0134994.1"
                     /translation="RGRVIPMTRLLMDMAVSARLRADLKAARDWPIYEELGADMIDVV
                     LDARYRIVRRIAKGGMADVFLAKDLTRKCPVAIKILHSRSPEAARRFRMEGIILSNLQ
                     HPGIVRAVDVGETPDGPPYIALEYLDGESLSVRVARGPLPWRDVATFGIQLAGAVHEL
                     HAAGIIHRDLKPANIMLTTGATGLSAAKLIDLGLARVGVPFLDAQDALFTPDPLERHQ
                     TQLGYPIGTPPYLPPEAGTCPADPQLDVYSLGATLYQLCTQQLPQATGGRPIREVCPG
                     SDAPDDLSRLLQAAFAPDPLERLATADHLQRGLEAILAAHPRAQGARRLFGGSYDLLE
                     VLGVGASAVAYRASDRRLSREVAVKVLRDAAPSEDDAIRFRRAAKVLSALRHPNIPQI
                     LHFGTHDHQTFAVTELCPGSPATIFVRPDNYLRPDEVIAVGLQLAGALAAGHAAGVVY
                     RDLHPGNVLIDRGDPIRAWIFDFDQAQVSAEFYAALTERWATPPEERAEPKRGKPLQR
                     MDYAAPEVRGGAAFSVASDVYALGLLLYRLLTGRRPFPVSGGEPTPPRELCPACPLEL
                     ERMLLTMLSPSPDNRPTLDVFQAALNEDVEDSDDAGAEAEAERPATGEHATAPAVATD
                     TVSAEPAAAFVSATDGAVTAVTDT"
BASE COUNT          270 a          657 c          726 g          294 t
ORIGIN      
        1 ctgtgtctgt cacggcggtc acggccccgt ctgttgcgct gacgaaagcc gccgcgggtt
       61 ccgccgacac ggtgtctgtc gctacggcgg gggccgtcgc gtgctcgcct gtcgcgggcc
      121 gctccgcttc cgcttccgcc ccggcatcgt cgctgtcctc gacatcttcg ttgagcgcgg
      181 cctggaacac gtcaagcgtc gggcggttgt cggggctcgg gctgagcatc gtcaggagca
      241 tgcgctcgag ctcaagcgga cacgccgggc acagctcgcg cggcggcgtc ggctcgcccc
      301 ccgacacggg gaagggccgc cgccccgtga gcaggcggta gagcaggagg ccgagcgcgt
      361 agacgtcgct ggcgaccgag aacgccgcgc ccccgcgtac ctcgggcgcg gcgtagtcca
      421 tgcgctgcaa gggcttgccg cgcttcggct cggcccgttc ctctggcggc gtcgcccacc
      481 gctcggtcag ggccgcgtaa aactcggccg acacctgcgc ctgatcgaag tcgaagatcc
      541 acgcgcgtat cgggtccccg cgatcgatca gcacgttgcc cgggtgcagg tcgcggtaca
      601 cgacgcccgc ggcatgcccg gcggcgagcg ccccggcgag ctgtaggccg accgcgataa
      661 cctcgtcggg gcgcaggtag ttgtcgggcc gaacgaagat cgtcgcgggc gagccggggc
      721 acagctccgt gaccgcgaag gtctgatggt cgtgagtccc gaagtgtagg atctgcggga
      781 tgttggggtg gcgtagcgcg gacagcacct tggccgcgcg ccggaagcgg atcgcgtcgt
      841 cctcgctcgg cgccgcgtcg cgtaggacct tgacggcgac ctcgcgggac agacggcgat
      901 cggaggcgcg gtaggcaacc gcgctcgcgc cgaccccgag aacctcgagt aggtcgtagc
      961 taccgccgaa caggcggcgt gcgccctgag cccgcgggtg cgcggcgagg atcgcctcga
     1021 gtccgcgttg caggtgatcg gcggtggcca ggcgctcgag tgggtccggc gcaaacgcgg
     1081 cctgaagcag ccgcgacagg tcgtccggcg catcacttcc ggggcagacc tcgcggatcg
     1141 gtcggccgcc cgttgcttgc gggagctgct gcgtgcagag ctggtagagc gtcgcgccga
     1201 gcgaatacac gtccagttgc gggtcggcgg ggcaagtccc ggcctcgggc ggcaggtagg
     1261 gcggcgtgcc gatcgggtac ccgagctgcg tctggtgccg ctcgagcgga tccggggtga
     1321 agagtgcatc ctgggcgtcc aggaacggca cgccgacccg ggcgaggccg aggtcaatca
     1381 gcttcgcggc ggacagcccg gtcgcgcctg tcgtcagcat gatgttggcg ggcttcaggt
     1441 cgcggtgaat gatgccggcg gcgtggagtt catggacggc gccggcgagc tggattccga
     1501 acgtcgccac gtcgcgccat ggcagcggtc cgcgcgcgac gcggacggac agcgactcgc
     1561 cgtccaggta ctcgagcgcg atgtacggcg gcccgtccgg cgtctcgccg acgtccaccg
     1621 cacggacgat cccggggtgt tgcaggttgc tgaggatgat cccttccatg cggaagcgcc
     1681 gcgcggcctc ggggctgcgc gagtggagga tcttgatcgc cacggggcac ttgcgcgtca
     1741 ggtccttggc caagaacacg tccgccatgc cgcccttcgc gatccggcgg acgatccggt
     1801 agcgagcgtc gagcaccacg tcgatcatgt cggcgcccaa ttcctcgtag atgggccagt
     1861 ccctcgcggc cttgaggtcg gctcgcaacc tggcactgac cgccatgtcc atgagcagcc
     1921 gggtcatggg gatcacccgg cccctcg
//
LOCUS       DASWNJ010000775         1823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_684239, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000775 DASWNJ010000000
VERSION     DASWNJ010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684239"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1591)
                     /locus_tag="VGB85_12965"
     CDS             complement(<1..1591)
                     /locus_tag="VGB85_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0134995.1"
                     /translation="MTRLTRLPLTALLGLLHACGDSSGGSDSQTSATTQTSVTTGVTV
                     TPTTGGPATASATGTDTGTDAGSATESASMTGEPTTGTATSTSSTTTGPTDPTATTDG
                     TTGPKFDVGETTDTTGDTGMPVDKCKATDDDMGNPGDCTETAPPNAFEPDVQWSWNGP
                     GAEKESLVTPLVANLTDDNGDGVIDLCDTPDIIVTVTAFPAPGHIYVLDGATGNLHFM
                     IPTAVTWSINPAIGDIDNDGIPEIVAATGEGFGGPSAAIAFEHDGTVKWQSQGQVSHS
                     QGGAITLADLDNDGDVEIILDRLILDHMGNTVATLAEVQVADNFTTVAADLDGNGDLE
                     VVIGPNAYHHDGSVLFQNPGFDRGFAQVANLDADPEPEIMINSYTGLTLLEHTGAVKY
                     QNIQPGGGQSWFRPGTVHDFDGDMVSEVATSAANSYVMFEGDGTVNWTAVVQDGSGWA
                     AGTAFDFDGNGIAEAMYADEINLYVFDGQGKPLLTVPRSAKTLSEYPVVADVDNDGSA
                     EIVVVSDAGFNNNQTAPTVQVI"
BASE COUNT          209 a          543 c          727 g          344 t
ORIGIN      
        1 ggatcacctg gacggtcggg gcggtctggt tgttgttgaa gccggcgtcg gagacgacga
       61 cgatctcggc ggagccgtcg ttgtctacgt cggcgacgac cgggtactcc gagagggtct
      121 tggcgctgcg cgggacggtc agcagcggct tgccctggcc gtcgaacacg tagaggttga
      181 tctcgtcggc gtacatcgcc tcggcgatgc cgttgccgtc gaagtcgaag gcggtgccgg
      241 cggcccagcc gctgccgtcc tgcacgacgg cggtccagtt gacggtgccg tcgccctcga
      301 acatcacgta gctgttggcg gcgctggtgg cgacctcgga gaccatgtcg ccgtcgaagt
      361 cgtgcacggt gccggggcgg aaccatgact ggccgccgcc gggctggatg ttctggtatt
      421 tgacggcgcc ggtgtgctcg agcagcgtca ggccggtgta gctgttgatc atgatctccg
      481 gctcggggtc ggcgtcgagg ttggcgacct gcgcgaagcc gcgatcgaag ccggggttct
      541 ggaacagcac gctgccgtcg tggtggtagg cgttggggcc gatgacgacc tcgaggtcgc
      601 cgttgccgtc gaggtcggcg gcgacggtgg tgaagttgtc ggcgacctgg acctcggcga
      661 gggtggcgac ggtgttgccc atgtggtcga ggatcaggcg gtcgaggatg atctcgacgt
      721 cgccgtcgtt gtcgaggtcg gcgagggtga tcgcgccgcc ctggctgtgc gagacctgac
      781 cctgactttg ccacttgacg gtgccgtcgt gctcgaaggc gatcgcagcc gacggcccgc
      841 cgaagccctc gccggtggcc gcgacgatct ccgggatccc gtcgttgtcg atgtcgccga
      901 tcgcagggtt gatcgaccag gtgacggcgg tcgggatcat gaagtgcagg ttgccggtcg
      961 cgccgtcgag cacgtagatg tgacccggcg ccgggaaggc ggtcaccgtg acgatgatgt
     1021 cgggggtgtc gcagaggtcg atcacgccgt cgccgttgtc gtcggtcagg ttggcgacca
     1081 gcggcgtgac gagcgactcc ttctcggcgc cggggccgtt ccagctccat tgcacgtcgg
     1141 gctcgaaggc gttcggcggg gccgtttcgg tgcagtcgcc ggggttgccc atgtcgtcgt
     1201 cggtcgcctt gcacttgtcg accggcatgc cggtgtcgcc ggtggtgtcg gtcgtctcgc
     1261 cgacgtcgaa cttggggccg gtggtcccgt ccgtggtcgc ggtcgggtcg gtggggcccg
     1321 tggtggtcga gctcgtcgag gtcgcggtgc cggtggtcgg ctcgccggtc atgctcgcgc
     1381 tctcggtcgc gctgccggca tctgtgccgg tgtcggtgcc ggtcgcgctc gcggtggcag
     1441 ggccgccggt cgtcggcgtc acggtgacgc cggtcgtcac ggacgtctgc gtggtcgcgc
     1501 tggtctggct gtcggagccg ccgctgctgt cgccacaggc gtgcaggagg ccgaggaggg
     1561 cggtgagagg gaggcgggtg aggcgggtca tgatggcgac ggtatgccgg ttgtctcgcg
     1621 gagaacatgc gcaaggccgc gcgcgcacgg acgttgacgc tggggcgggc agcggccagc
     1681 atcatggctt gccgatgtcg gacgcacgct tccccggaat gctccccgag ctggtgcagc
     1741 gggtgtgcgc cgtcgcggtg ccgccgtatc tgcgggcgct gtttcgccgc ctcggtgtgc
     1801 cggtcgagct gatcgtcgag gcg
//
LOCUS       DASWNJ010000776         3641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_14713, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000776 DASWNJ010000000
VERSION     DASWNJ010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14713"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VGB85_12970"
     CDS             complement(<1..727)
                     /locus_tag="VGB85_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134996.1"
                     /translation="MITASSRPITMLGTSAAIAGFDRDMRHERTVFAGGGRGLELAAS
                     REEAVARGRMLVSEFHARTHGGVLVTETVPYDATAVRGSLRWLHGALDLAKDAAPPPG
                     LGPLAAPRSKAEECAHCKTYVSTQVSRRKDAPDERVCRRCFELAQAGRAQQGDLAERI
                     QSLVNLDPEGRWIAAVSIDGNNLGDFFQGLDSLERMREASEALSSLFKKADTDARGRL
                     KHDVISLATGGDDLRLFMAGADLL"
     gene            complement(1081..2559)
                     /gene="csb2"
                     /locus_tag="VGB85_12975"
     CDS             complement(1081..2559)
                     /gene="csb2"
                     /locus_tag="VGB85_12975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Csb2"
                     /protein_id="HEY0134997.1"
                     /translation="MIALAFTLLAGRYHATAWDHHVNEGTVEWPPSPWRLLRTLVAAS
                     YRVGPEFDRRRLEAVLERLTVPPVYYLPPIAPGHLRHYVPIEGSNPTRVFDAFLAVGG
                     GAATPGEIVVAWPGLEFDAADRALLAQVAAQVSYLGRAESWAEARVCAELPGRPPDAR
                     PRDPGEDLPAQTRLLAPLSASEYQLWRDGFLAAQGPRQRGKSSLPETLWDRLNLDTAT
                     LQRDGWSAVPGTRWIPYAITDAPVLARHRPAPARSDTPNFVRLVLDGPVHPHIERSLW
                     IGEKLRAALLSHVGDRPCPVLTGKDADGRPLQDHGHAYFLPQANERGEVHHVLIHARD
                     GFDAHALHALRSLRVVHGLTSHPVYTTVLALGRSEAFEQPPLRAATRWESLTPFVAPR
                     CPKQRHGEWLDTPEAQLRRLCRLVLGVEPTAIDPFTPTETRERRLYAYRRERRRGGPV
                     PGHATILGFRLEFATPVRGPIALGYGAHFGLGQFTPLNRPLD"
     gene            complement(2556..3038)
                     /locus_tag="VGB85_12980"
     CDS             complement(2556..3038)
                     /locus_tag="VGB85_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134998.1"
                     /translation="MNFASLFREITGQYPYPYQALLATSGDLPDLFSIPTGAGKTAAI
                     VLGWLWGRRFADVETRGVDEDRVVVRGQLLALVDGVAEQGVDSHRRFKARPAVAEALP
                     GLLNAAARTGVFADPPLRRAVFQAPKRGKSRDADGVTASDDEPAPTGKKPASRKKAGQ
                     "
BASE COUNT          576 a         1173 c         1357 g          535 t
ORIGIN      
        1 tgagcaggtc cgcccctgcc atgaacaggc gcaggtcatc gccgccggtc gccagcgaga
       61 tcacgtcgtg cttgagccga ccacgagcgt cagtgtcggc cttcttgaac aggctcgaca
      121 gcgcctcgct ggcttctcgc atccgttcga gtgaatcgag gccctgaaag aaatcaccga
      181 ggttattgcc gtcgatcgag acggcggcga tccagcgccc ctcgggatca aggttgacga
      241 gcgactggat ccgctccgcg aggtccccct gctgcgcgcg cccggcctgc gccagttcga
      301 agcagcgccg gcacacccgc tcatcggggg catccttgcg tcgtgacact tgcgtgctca
      361 cgtacgtctt gcaatgggcg cactcttcgg ccttgctacg cggggcggcg aggggcccca
      421 ggccgggagg cggagcggcg tccttcgcca agtcgagggc gccgtgcaac cagcgtaggc
      481 tgccccgtac ggcggtggcg tcgtaaggca ccgtttcagt caccaggacg ccaccgtggg
      541 tacgtgcgtg gaactcgctg accagcatac gtccacgcgc aacggcctcc tctcggctgg
      601 ccgccagctc aaggccgcgg ccacctcccg cgaagactgt ccgctcgtgc cgcatgtcgc
      661 ggtcgaaacc ggcgatcgcc gcgctggtgc caagcatggt gatggggcgc gagctcgcgg
      721 tgatcaactc ctggatacca ttggcgtcgt atccgaggag ccacggccgc ggcccggcac
      781 cccgcagggc gcgagcaaga gaagttgagt cgggtttatc catcgatatt ctcgggagcc
      841 gtgggtcacg tggggaggat tctctggagt cgtcgctcgc tgccgatcga tcctggcgac
      901 ggcgaaatct aattcgcagg cggggatgag tggtccgagc cggagcatcg ccctggcgtt
      961 gacgaaggca gctctgtagc tgcacgcggc tcgtccgggc gaaaggctcg accgcgtcgg
     1021 ctcgacgctg tcatcaggcg ccagatgtgg cgcatcgggt cgataaggta tggcagtcgc
     1081 tcaatccagc ggtcggttga ggggggtgaa ctggccgagg ccgaagtggg cgccgtaacc
     1141 gagggcgatc gggccgcgca cgggggttgc gaactcgagg cgaaagccga ggatcgtcgc
     1201 atgcccgggg acgggtccgc ccctccgacg ctcgcggcga taggcgtaaa ggcggcgctc
     1261 gcgggtctcc gtgggggtga aggggtcgat cgcggtcggc tcgacgccga ggacgaggcg
     1321 gcagaggcgg cgtagctggg cctcgggggt gtccagccac tcgccgtggc gctgcttggg
     1381 gcagcggggc gcgacgaacg gcgtgaggct ctcccaccgg gtcgcggcgc gcagcggcgg
     1441 ctgctcgaag gcctcggagc gaccgagcgc gaggacggtc gtgtacaccg gatgactggt
     1501 cagaccatga acgacgcgca gggagcggag cgcgtgcagg gcgtgggcgt cgaagccgtc
     1561 gcgggcgtgg ataaggacgt ggtgcacctc gccgcgctcg ttggcctgcg ggaggaagta
     1621 ggcgtggccg tggtcctgca gcgggcggcc gtcggcgtcc ttgccggtga gcacggggca
     1681 aggccgatcg ccgacgtggg agagcagcgc ggcgcgcagt ttttcgccga tccagagcga
     1741 cctctcgatg tgggggtgga cgggaccgtc gaggacaagg cgaacgaagt tcggggtgtc
     1801 ggagcgggcg ggcgcggggc ggtggcgggc gagcacgggc gcatcggtga tggcgtaggg
     1861 gatccagcgg gtgccgggga cggcggacca gccgtcgcgc tggagcgtcg cggtgtcgag
     1921 gttgaggcgg tcccagaggg tctcggggag cgacgatttg ccgcgctgcc gcggaccctg
     1981 ggcggcgagg aagccgtcgc gccacagctg gtattcggac gcggagagcg gcgcgagcag
     2041 gcgcgtctgg gccggcagat cttcgccggg gtcgcgggga cgggcgtccg gcgggcgccc
     2101 ggggagctcg gcgcagacgc gggcctcggc ccagctctcg gcgcgaccga ggtagctgac
     2161 ctgcgcggcg acctgggcga ggagcgcccg atctgcggcg tcgaactcga gcccgggcca
     2221 ggccacgacg atctccccag gcgtcgcggc gccgccacca acggcgagga acgcgtcgaa
     2281 cacccgggtc gggttgctgc cctcgatcgg cacgtagtga cgcaggtggc cgggcgcgat
     2341 cggcgggagg taatagactg gtgggacagt caagcgctcg agcacggcct ccaggcggcg
     2401 acgatcaaac tccgggccga cacggtagct ggcggcgacg agcgtccgga gcagacgcca
     2461 cggtgaaggc ggccactcga cggtgccctc gttgacgtgg tgatcccagg cggtggcgtg
     2521 gtagcggccg gccaggaggg tgaatgcgag ggcgatcact gcccggcctt cttccggctg
     2581 gcgggcttct tgccggtcgg cgcgggctcg tcgtcgctgg cggtcacacc gtccgcgtcc
     2641 ctggacttgc cgcgcttggg cgcctggaac accgcgcgac ggagcggcgg gtcggcgaac
     2701 acgccagttc gggcggcggc gttgagcagg ccggggagcg cctctgcaac cgccgggcgc
     2761 gccttgaatc ggcggtggct gtccacgccc tgctcggcga cgccgtcgac gagcgctaga
     2821 agctggcccc ggaccacaac gcgatcctca tcgacgccgc gagtctccac atccgcgaag
     2881 cggcgccccc atagccaccc gaggacgatg gccgcggtct tgccagcgcc cgtcgggatc
     2941 gagaacaggt caggaagatc accgctcgtg gcgagcaggg cctgatacgg atacggatat
     3001 tggccggtaa tctcccggaa caaggacgcg aaattcacat aacctccgcg gatacggcat
     3061 ccctcgggac gcatggcagc gcattcaaat cgtcggttac gacgctctgg atcgatgctt
     3121 cagggaagca tcggtccact gccctgggga tgagtcgcgc gcgcgagaag ttcgatcccc
     3181 aaaatcgccc ggggcgggaa acttcggtcg cggtcgaggg tgcaacatgt tcctgtggac
     3241 ccgcccgcgc gacacgtccg aggatgtcga gcctcagcga gacgtgcggc agcctctccc
     3301 tcaccccccg atgcgcctgt cgaaggctcc acgggcagtg accaggcggc acccggttca
     3361 ctgagcaact ctcacaagtg ttgacgacat gacccatgca ccccgcccga caaaatcgca
     3421 cagcgcggta acgctcgcaa gtttcggccg caaacccaaa ataggtgccg tcagagcacg
     3481 tcgcgccatc ggctggtttc gctacgacgt cgcccgggcc tcagccctcg ccgtcatccg
     3541 cgggcttcgg cttgtcggct ggcggatggg gcccccggct ctcgagcagt ctgcaccgcc
     3601 gctccggacc cctccggcaa ccccatggtg cagagattag g
//
LOCUS       DASWNJ010000777         3052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_650380, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000777 DASWNJ010000000
VERSION     DASWNJ010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650380"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VGB85_12985"
     CDS             <1..377
                     /locus_tag="VGB85_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0134999.1"
                     /translation="TFAGSVPAIVAQSAYTFAGPQATVTTTALQRLTGAATTSLGAAA
                     NIPNVRVDLCYQPNGGGVLTNFSGFAYVEHPFTVARVPYTAVGSVVPGAGTWKVGLCV
                     YNPTATSINQTNYMNGWVQVTN"
     gene            408..866
                     /locus_tag="VGB85_12990"
     CDS             408..866
                     /locus_tag="VGB85_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135000.1"
                     /translation="MGSKTSMRTAILEGTPGVAVCSFVAAMLVGCGAPTPEQPVQPEK
                     SSMPVSPGTPVSRETATASAIEALSYHRPALRARCYEPAVAGLGDRPVYRFMLDVTFD
                     AAGAPISWGITEDRETSPGAVTQCVIDHLPALYIPPPGQVTRVELPLQLP"
     gene            895..1515
                     /locus_tag="VGB85_12995"
     CDS             895..1515
                     /locus_tag="VGB85_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135001.1"
                     /translation="MAERRTWILISLLMASAGVMVLALTIFGRREPDPVVATTKEPGP
                     QSLGVRAAGADSARRVEAGEDVEQPLPALPAVAPVEVVDESSRPGPPPEPAVPIVPSA
                     EDVAASDAAMEVMPRVKAEVEEAIESRRKSLRNACWNSVKLASATFPMEATFAADGGL
                     LAFSIGEDRGAPEVGACIRSQPRSLVPLKIAAPGVPITVKVALTLP"
     gene            1673..2251
                     /locus_tag="VGB85_13000"
     CDS             1673..2251
                     /locus_tag="VGB85_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135002.1"
                     /translation="MRRAAHMSMWWGPVVMVGALLSGACGDDGIAMTEGVTESGTSSG
                     TTDTPATETTPTTTDPTETTETPTTTPTTSDTSTTSPVTSSTTDETTGTESDTDTTGT
                     TTTGNTTGDTTDGTTGTTTGDTTTGDTDTDTGNGVMGHSVSQLVNGGTVASSANFRMV
                     FTIGQPTQNQGTYKSDNFRLQGGLIGANGNPP"
     gene            2248..>3052
                     /locus_tag="VGB85_13005"
     CDS             2248..>3052
                     /locus_tag="VGB85_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135003.1"
                     /translation="MKKNYSGMRKWLMGSAVAAATLFTAMDARADVPQVVTHQGRLFD
                     TNNMPVDGTQDLKFTIYDAEDAGNELWSETITVDFDDGFFSVQLGDLVPLDEVVFDGS
                     IRFLGVTVGADAEMTPRAPVASVPYAMFAGDVRGDIHPVSVDIQGFGQVIDANGQWVG
                     DPSGLVGPAGPAGPAGPAGAAGAAGPAGPKCDPGAAGPAGPAGPAGPVGPAGPAGPAG
                     AVGPAGPAGPAGAVGPAGPAGPAGAMGASGPAGPAGPMGPAGAMGATGPA"
BASE COUNT          483 a          991 c         1139 g          439 t
ORIGIN      
        1 gcacctttgc gggcagcgtg ccggcgatcg tcgcccagag cgcgtacacc ttcgcggggc
       61 cgcaggcgac cgtcaccacg acggcgctgc agcgcctgac gggcgcggcg acgacctcgc
      121 tcggcgcggc ggccaacatc ccgaacgtgc gcgtcgacct gtgttaccag cccaacggcg
      181 gcggcgtgct gaccaacttc agcggcttcg cgtacgtcga gcacccgttc acggtcgccc
      241 gcgtgccgta cacggcggtc ggcagcgtcg tccccggcgc cggcacctgg aaggtcggcc
      301 tgtgcgtcta caaccccacc gcgacctcga tcaaccagac gaactacatg aacggctggg
      361 ttcaggtcac gaactgacgt gataggcttg cgacgagggc gctcgtcgtg ggctcgaaga
      421 catcgatgcg gaccgcgatc ctcgagggta ccccgggggt cgcggtctgt tcgttcgtcg
      481 ccgcgatgct cgtgggttgc ggcgcgccga caccggagca gccggtgcag ccggagaagt
      541 cgtcgatgcc ggtgtcgccg ggcacgccag tatcgcggga gacggcgacg gcgagcgcga
      601 tcgaggcgct gtcgtatcac cgtccggccc tacgcgcgcg ctgctacgag cccgcggtcg
      661 ccggcctcgg cgaccgcccg gtgtaccgct tcatgctcga cgtgaccttc gacgccgcgg
      721 gcgccccgat ctcgtggggg atcacggagg accgcgagac ctcgcccggc gcggtcacgc
      781 agtgcgtgat cgatcatctt ccggcgctgt acatcccgcc gcccgggcag gtgacgcgcg
      841 tcgagttacc gcttcagctc ccttgatcat gcgggggaca gcgaaggagt cggtgtggcc
      901 gagcgacgaa cctggatcct gatctctctg ctgatggcga gcgccggcgt catggtgctc
      961 gcattgacga tcttcggacg acgcgagccg gacccggtcg tggcgacgac aaaggagccc
     1021 ggaccgcagt cgctcggcgt gcgagccgca ggcgccgact ccgcgaggcg ggtcgaggcc
     1081 ggtgaggatg tcgagcagcc gttgccggcg ctaccggcgg tcgctccggt cgaggtcgtc
     1141 gacgagtcga gccgtcccgg gccgccgccc gagccggccg tgccgatcgt cccgagcgcg
     1201 gaggacgtgg ccgcctccga cgcagcgatg gaggtcatgc cccgcgtcaa ggcggaggtc
     1261 gaggaggcga tcgagtcgcg tcgcaagtca ctccgcaacg cgtgttggaa cagcgtcaaa
     1321 ctggcctccg cgacctttcc gatggaggcg accttcgccg cggacggcgg tctgctggcg
     1381 ttcagcatcg gtgaagaccg cggagcaccc gaggtcggcg cgtgcattcg gagccagccg
     1441 cgatcgctcg ttcctctcaa gatcgcggcg ccgggtgtac cgatcacggt gaaggtcgcg
     1501 ttgaccttgc cgtaatgagg acgtcgctcg cggacggcga gcggaggcgc gcggtcgatc
     1561 tccacctgtc ttgaaggaca ggcagcgagt tgtcgcgtcg ctcgcttcct gttacggtcg
     1621 gcgcgttagt tgggttgtga tgccgagggg acaacaaaca ggagtggata acatgcgccg
     1681 agccgcacac atgagcatgt ggtggggtcc ggtcgtgatg gtgggagcgc tgctcagcgg
     1741 ggcgtgtggc gacgacggga tcgccatgac ggagggtgtg acggagagtg ggacatcttc
     1801 ggggacgacg gacacgccgg ccacggagac cacgccgacg acgacggatc cgacggagac
     1861 gacggagacg ccgacgacca cgccgacgac gagcgacacc tcaacgacct cgccggtcac
     1921 gtcctcgacg acggacgaga cgacggggac ggagagcgac accgacacga cgggaacgac
     1981 gacgacagga aacacgacgg gtgacacgac ggacggaacg accgggacga cgaccgggga
     2041 cacgacgacg ggtgacacgg acacggatac gggtaacggg gtgatgggtc actcggtgag
     2101 ccagctggtg aacggcggga cggtggcgag cagcgccaac ttccgcatgg tcttcacgat
     2161 cggccagccg acgcagaacc aggggactta caaatctgac aacttccgac ttcagggcgg
     2221 cctgatcgga gccaacggga atcctccatg aagaagaatt acagtggaat gcggaagtgg
     2281 ttgatgggct ccgcggtggc ggcggcgacc ttgttcacgg cgatggacgc gcgcgccgac
     2341 gtgccgcagg tggtcaccca ccagggccgt ctgttcgaca cgaacaatat gccggtggac
     2401 ggtacccagg acctcaagtt caccatctac gacgcggagg acgccggcaa cgagctgtgg
     2461 tcggagacga tcacggtcga cttcgacgac ggcttcttct cggtgcagct cggcgatctg
     2521 gtgccgctcg acgaggtggt gttcgacggg tcgatccggt tcctcggcgt caccgtcggg
     2581 gccgacgcgg agatgacccc gcgagccccg gtcgcgtccg tcccctacgc gatgttcgcg
     2641 ggtgatgtcc gcggcgacat ccatcccgtc agcgtcgaca tccagggctt cggccaggtc
     2701 atcgacgcga acggccagtg ggtcggcgac ccgagcggcc tcgtcggtcc ggcgggtccg
     2761 gcgggtcccg cgggtcccgc cggtgcggcc ggtgcggccg gtcctgccgg tccgaagtgc
     2821 gacccgggcg cggcgggtcc agcgggtcca gcgggtccag cgggtccggt cggcccggcg
     2881 ggtccagcgg gccctgcggg tgcggtcggc ccggcgggtc cagcgggccc tgcgggtgcg
     2941 gtcggcccgg cgggcccggc gggtccagcg ggtgcgatgg gcgcgtcggg tccggcgggt
     3001 ccagcgggcc cgatgggtcc tgcgggtgcg atgggcgcga cgggccctgc ag
//
LOCUS       DASWNJ010000778         4066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_49955, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000778 DASWNJ010000000
VERSION     DASWNJ010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49955"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VGB85_13010"
     CDS             <1..695
                     /locus_tag="VGB85_13010"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0135004.1"
                     /translation="TAEEFPQVAASWWNIIHPEDMDKSTGAIVAHLADPRTPMYVQYR
                     VRTRQGGYRWWEIRGSTMPDTIPGRIRAMGVARDITTTKQAEETLREQLGVITEQQTA
                     IRSMSTPIIQVWDGILTLPLIGTFDTDRATEIMERLLSEVTRLRARHAILDLTGIETV
                     DTTTAEQLYRIIRAVGLLGARAVLSGLNPAIAQTLTELGVDTSRFVTYRNMQGALQAC
                     IEQQARRNRTSR"
     gene            692..1324
                     /locus_tag="VGB85_13015"
     CDS             692..1324
                     /locus_tag="VGB85_13015"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0135005.1"
                     /translation="MNQRAELDERTIVAVLGKYVSPARAQTIVTRARREPVTPFGRSG
                     ADTAGLMLRIAEGLRIFLGETDTRAAIADLQRHASGKKAEPVELDLRVESDIARARSA
                     ARTICEALGASPLLLQKLVTIVSELARNSVIYAGGGKLTLRPDPSTRRVYIEAKDQGP
                     GIPNLEEILAGRYKSRSGLGLGILGTRRLADGFSIQSSSAGTHVKVEVAY"
     gene            1347..1796
                     /locus_tag="VGB85_13020"
     CDS             1347..1796
                     /locus_tag="VGB85_13020"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0135006.1"
                     /translation="MSNYENRIPVIQLWQRIVVPLQGDIADETAAILVDDVLRTIRDT
                     SAAGLIIDVTGMWTMDSHLCHLIASLAAAARLMGTPTVICGMSAPIAMTLQTMGADFR
                     AAQTALVLEDALERLGVTATVTEIDPDAEFEDSQRLDSAFGGDAFQG"
     gene            complement(1816..2595)
                     /locus_tag="VGB85_13025"
     CDS             complement(1816..2595)
                     /locus_tag="VGB85_13025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0135007.1"
                     /translation="MKDRVIVITGGSAGIGAALAEEVARRGARPVIAARRAGPLAEVA
                     ARIEGAIAIVTDVTRREEVEHLMAEAVARAGRVDVWVNNAGRGISRAVSELTDEDLDE
                     MFLVNVKSALYGMQAVLPHFKARGTGHIINVSSMLGRVPLAVVRSAYSAAKHALNSLT
                     TNLRMELRDTHPGIHVTTVLPGVVATDFGTHARHGGMDSRSLPGAQDVEEVSRVIADA
                     IESPRAEVYTRPEYHKMSAAYYAAEDVAVLEGQPPFVAPRR"
     gene            2672..3616
                     /locus_tag="VGB85_13030"
     CDS             2672..3616
                     /locus_tag="VGB85_13030"
                     /inference="COORDINATES: protein motif:HMM:NF015500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HEY0135008.1"
                     /translation="MQHRWRAPWLLTVCLGCATSPADTTDADTTGPQPTSDTTDTTST
                     TEPTSASTSEPTSSSTTGEDPPGTTGTTGDPPPVMLPPADAGLDYQLGGAYPPPAGVG
                     IVSRDRNAPPAGGLYNICYINGFQTQPDEESFWLDEHPELVLRDASDAPVIDEDWGEM
                     LLDTRTPESRAALAQIVGGWITDCGAAGYQAIEIDNLDSYARSGGLLDQDQAVAYMAM
                     LSQAAHAGGMAIAQKNSSELLPRRAEMATDFVVSEECNQYDECDDYIATYGDAVVIIE
                     YDDAAFAEGCAAYPQLSIVRRDLQLTTPGDADYVFDAC"
     gene            3647..>4066
                     /locus_tag="VGB85_13035"
     CDS             3647..>4066
                     /locus_tag="VGB85_13035"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0135009.1"
                     /translation="MPRCLALALLFLACARPEPAAHVEPVEPVQPAQPVQPAAPTART
                     PAPAMSYLGADWLTRSERVAEEQPDAMLDALRITPGMRVADIGAGVGYHALRIAPRVR
                     PGGTVYATDLQPEMLEMLRAAVRDAGAGDIEAVLSSDR"
BASE COUNT          643 a         1602 c         1255 g          566 t
ORIGIN      
        1 acacagcgga ggagttcccg caggtcgccg cgagctggtg gaatatcatc catcccgaag
       61 atatggataa gtcgaccggg gcgatcgtcg cccacctcgc cgatccccgg accccgatgt
      121 atgtccaata ccgggtccgc actcgacagg gtggttatcg ctggtgggag atccgcggca
      181 gcaccatgcc ggacaccatc cccggccgga tccgtgcgat gggcgtcgcc cgcgacatca
      241 ccaccacgaa gcaggccgag gagaccctgc gcgagcagct cggcgtgatc accgagcagc
      301 agaccgcgat ccgctcgatg tccaccccga tcattcaggt ctgggacggc atcctaacgt
      361 tgcctttgat cggcaccttc gacaccgacc gcgcgaccga gatcatggag cgcctgctct
      421 cggaggtcac ccgcctgcgg gcccgccacg cgatcctcga cctcaccggc atcgagaccg
      481 tcgacacgac caccgccgag cagctctatc gcatcatccg cgcggtcggc ctgctcggcg
      541 cccgcgcggt gctcagcggc ctcaacccgg cgatcgccca gaccctgacc gagctcggcg
      601 tcgacacctc gcgcttcgtc acctatcgca acatgcaggg cgccctccag gcctgcatcg
      661 aacagcaagc ccgccgcaat cggacgtccc gttgaaccaa cgcgcagagc tggatgagcg
      721 gacgatcgtc gccgtgctcg gcaagtacgt cagtcctgcg cgcgcgcaga cgatcgtcac
      781 gcgcgcccgc cgggagcccg tcacgccgtt tggccgcagc ggcgccgaca cggccggcct
      841 gatgctgcgc atcgccgagg gcctgcgcat cttcctcggc gagaccgaca cccgcgccgc
      901 gatcgccgat ctgcagcgtc acgccagcgg caagaaggcc gagcccgtcg agctcgacct
      961 gcgcgtcgag tccgacatcg cccgcgcccg ctccgccgct cgcacgatct gcgaggcgct
     1021 cggcgcctcc ccgctgctgc tgcagaagct ggtcaccatc gtcagcgagc tggcccgcaa
     1081 cagcgtcatc tacgccggtg gcggcaagct gaccctgcgc cccgacccca gcacccgtcg
     1141 cgtgtacatc gaggccaagg accagggtcc cggcatcccc aacctcgagg agatcctcgc
     1201 aggtcgttac aagagccgca gcggcctcgg gctcggcatc ctcgggaccc gacgcctcgc
     1261 cgacggtttt tccattcagt cgagctccgc cgggacccac gtcaaggtcg aggtcgcgta
     1321 ttgatgtccc ccacccccat aaagccatga gcaactacga gaaccgcatc cccgtcatcc
     1381 agctgtggca gcgcatcgtc gtcccgcttc agggcgacat cgccgacgag acggccgcga
     1441 tcctcgtcga cgatgtgttg cgcacgatcc gtgacacctc cgcggctggc ctgatcatcg
     1501 atgtcaccgg catgtggacg atggacagcc acctgtgcca cctcatcgcc agcctggccg
     1561 ccgccgcccg actcatgggc accccgaccg tcatctgtgg catgagcgcg ccgatcgcca
     1621 tgaccctgca gacgatgggc gccgacttcc gcgccgccca gaccgccctc gtcctcgagg
     1681 acgccctcga gcgactcggc gtcaccgcga ccgtcaccga gatcgatccg gacgccgaat
     1741 tcgaggactc ccagcgcctc gacagcgcct tcggcggcga cgccttccag ggctgaaaga
     1801 ggccccgcat cgtcgtcacc ggcgcggcgc cacgaacggc ggctgaccct cgagcaccgc
     1861 gacatcctcg gccgcataat aggcggccga catcttgtga tactccggcc gcgtatagac
     1921 ctccgcgcgc ggcgactcga tcgcgtcggc gatcacgcgc gagacctcct cgacgtcctg
     1981 cgcgccgggc aagctgcgcg agtccatccc gccgtgccgc gcgtgcgtgc cgaagtcggt
     2041 ggcgaccacg cccggcagga ccgtcgtcac gtggatgccg ggatgggtgt cgcgcagctc
     2101 catccggaga ttggtcgtca gggaattgag cgcgtgcttg gccgcgctgt acgccgagcg
     2161 gaccaccgcg agcggcacgc ggccgagcat cgagctgacg ttgatgatgt gccccgtccc
     2221 gcgcgccttg aagtgcggca acacggcctg catcccgtac agcgcggact tcacgttgac
     2281 gaggaacatc tcgtcgaggt cctcgtcggt caactccgag acagcccgcg agatcccccg
     2341 acccgcgttg ttgacccaca cgtcgacacg cccggcgcgg gcgaccgcct ccgccatcag
     2401 gtgctcgacc tcctcgcgcc gcgtgacatc ggtgacgatc gcgatcgcac cctcgatgcg
     2461 cgcggcgacc tcggcgagcg gcccggccct gcgcgccgcg atcaccggcc gcgctccacg
     2521 acgcgcgacc tcctccgcga gcgccgcacc gatgcccgcg ctgccgcccg tgatgacgat
     2581 gaccctgtcc ttcatggcgc gactgtaccg caggacaggc ccgccggccc gcattgcccc
     2641 gccccgccgg cccgtgcgac cctgccgcat catgcagcat cgctggcgcg ccccctggtt
     2701 gttgacggtc tgcctcggtt gcgcgacctc ccccgcggac accaccgacg ccgacacgac
     2761 cggcccgcag ccgacctcgg acaccaccga caccacctcg accaccgagc cgacgagcgc
     2821 ctcgacctcc gagccgacga gctcctcgac cacgggcgag gacccgcccg gcaccaccgg
     2881 caccaccgga gatcctccgc ccgtcatgct cccgccggcc gacgccggcc tcgattacca
     2941 gctcggcggc gcctacccgc cgccggccgg cgtcggcatc gtctcgcgcg accgcaacgc
     3001 cccgcccgcc ggcggtcttt ataatatctg ttacatcaac ggcttccaga cccagcccga
     3061 cgaggagtcc ttttggctcg acgagcaccc cgagctggtc ctgcgtgacg caagcgacgc
     3121 cccggtgatc gacgaggact ggggcgagat gctgctcgac acgcgcacgc cggagtcacg
     3181 cgccgccctc gcgcagatcg tcggcggatg gatcaccgac tgcggcgccg cgggctatca
     3241 ggcgatcgag atcgacaacc tcgactccta cgcgcgctcc ggcgggctgc tcgatcagga
     3301 ccaggccgtc gcctacatgg cgatgctctc ccaggcggcg cacgccggcg gcatggcgat
     3361 cgcccagaag aattccagcg agctgctgcc ccgccgcgcc gagatggcga ccgacttcgt
     3421 cgtctccgag gagtgtaatc aatacgacga gtgcgacgat tacatcgcga cctacggcga
     3481 cgccgtcgtg atcatcgaat acgacgacgc cgccttcgcc gagggctgcg ccgcctatcc
     3541 gcagctctcg atcgtccggc gcgacctcca gctgaccacc cccggcgacg ccgactacgt
     3601 gttcgacgcc tgctgatccc gcacacccga cctatactga ccggcgatgc cccgctgcct
     3661 cgccctcgcg ctgctgttcc tcgcctgcgc ccgccccgag cccgccgccc atgtcgagcc
     3721 ggtcgagccg gtccagccgg cccagccggt ccagccggcc gcgcccacgg cacgcacgcc
     3781 ggcgccggcg atgagctacc tcggcgccga ctggttgacc cgcagcgagc gcgtcgccga
     3841 ggagcagccg gacgcgatgc tcgacgcgct gcgcatcacg ccgggcatgc gggtcgccga
     3901 catcggcgcc ggcgtcggct atcacgcgct gcgcatcgcc ccgcgcgtgc gcccgggcgg
     3961 caccgtgtac gcgaccgacc ttcagccgga gatgctcgag atgttgcggg ccgcggtgcg
     4021 tgacgccggc gccggcgaca tcgaggcggt gctctccagc gaccgc
//
LOCUS       DASWNJ010000779         2850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_366931, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000779 DASWNJ010000000
VERSION     DASWNJ010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366931"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VGB85_13040"
     CDS             complement(<1..714)
                     /locus_tag="VGB85_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HEY0135010.1"
                     /translation="MKPATSPLGIFERYLSVWVALAITAGVGLGNLFPGLFTVISRLE
                     YANVNFVVAILIWVMIYPMMIQIDFGAVRDVRRRPKGLIITLVVNWLIKPFTMAALAS
                     LFFLHVFDAWIPAAEAREYIAGAILLGVAPCTAMVFVWSQLTRGDANYTLVQVAVNDL
                     VMVVAFAPLTALLLGVSDIVVPWTTLLLSVVLYVVIPLLAGVATRRALTRRISDPEAL
                     GRFVGHLKPAGILGLLATVV"
     gene            complement(780..1115)
                     /locus_tag="VGB85_13045"
     CDS             complement(780..1115)
                     /locus_tag="VGB85_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY0135011.1"
                     /translation="MKTPAPCCPPAADEPDLRPVQGDAADEELAALAKALGHPIRVQI
                     VRLLTRRESCICGDIVKELPLAQSTVSQHLKVLKEAGLIRGEIDGPRTCYCLEPRTLR
                     RLKALVGGL"
     gene            complement(1431..1856)
                     /locus_tag="VGB85_13050"
     CDS             complement(1431..1856)
                     /locus_tag="VGB85_13050"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY0135012.1"
                     /translation="MRLTGLRPLALSLLLACACSDTGGGVGGVEVAVAGSARTWTHAE
                     LVAMPTASVAYHGKHYTGVPLPALVSALEASADRPLTAVASDGYTQTLAFEILAHTDA
                     LLAHTVDGAPLPASEGPLRLVVPGAEGLSVKQLVRLEQP"
     gene            2026..2694
                     /locus_tag="VGB85_13055"
     CDS             2026..2694
                     /locus_tag="VGB85_13055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0135013.1"
                     /translation="MANDQDPLGAKPQTCDDLGRDGLRRAPDFLASLARDVRGPLVSL
                     ATALAVFERYAPRDSNEERAREVARRATAALGHIANLIDPWSHVEVLPPVVDESPRSR
                     PTRVLIIEDNEDLRTILTDLCRLLGHDVCAAGDGVDGVATALRQVPDIALIDIGLPDI
                     DGCEVARRIREDPRGAGIQLVAMTAYSSAGQRASAAAAGFRLYVLKSASIEELVAALA
                     ACTS"
BASE COUNT          494 a          941 c          959 g          456 t
ORIGIN      
        1 cacgaccgtg gccaggagac cgaggatgcc cgccggcttc agatggccca cgaagcgccc
       61 gagggcctcg gggtcgctga ttcggcgagt caacgctcgt cgggtggcga cgcccgcgag
      121 cagcgggatc acgacgtaga gaaccacgga gagcagcagc gtcgtccacg gcacgacgat
      181 gtccgagacg ccgagcagca gcgccgtgag cggggcgaag gccaccacca tcacgaggtc
      241 gttcaccgcg acctgcacca gcgtgtagtt ggcgtcgccg cgggtgagtt ggctccacac
      301 gaacaccatc gcggtgcaag gcgcgacgcc cagcaggatc gctccggcga tgtactcgcg
      361 ggcctcggcg gcggggatcc aggcgtcgaa cacgtgcagg aagaacaggc tcgccagcgc
      421 tgccatcgtg aacggcttga tcagccagtt gacgacgagg gtgatgatca ggcccttcgg
      481 tcggcgtctg acgtcacgga cggcgccgaa gtcgatctgg atcatcatcg ggtagatcat
      541 cacccagatc aggatcgcta cgacgaagtt gacgttggcg tactccagcc ggctgatcac
      601 ggtgaacaga ccggggaaca agttgccgag gccgacgccg gcggtgatgg ccagggccac
      661 ccacacgctg aggtagcgct cgaagatgcc gaggggactg gttgcgggct tcatcggtcg
      721 gtgctccagg gtagccacgg ggcttcgcag ggtctcaagt tcgtattttt acgatggtgc
      781 tagaggccgc cgacaagcgc cttgaggcgc cgcagcgtgc ggggttcaag gcaatagcaa
      841 gtgcgcgggc cgtcgatctc gccacggatg aggcccgcct ctttaagcac cttcaggtgc
      901 tgcgagaccg tagactgggc caggggcagc tctttgacga tgtcgccgca aatgcaggac
      961 tctcgccgcg tcagcagccg gacgatctgc acgcggatcg ggtgtccgag cgcctttgct
     1021 agcgcggcca gctcctcgtc ggccgcgtca ccctggaccg ggcggaggtc cggctcgtcg
     1081 gcggccgggg ggcagcatgg cgctggtgtc ttcatcgtta attaacgata tagaacgctt
     1141 tagccggcac agcaagctcc gagtgatgcc ccgagcatgc gcccgccgag cactccggcg
     1201 gggtgtgcag tacctgccgt ccgcggcgat gcggtgacga accgggtacg ctcctcttct
     1261 cgaacgccga tgcgccaggc gtcgcccatg ctgatctgcg ccagatacgt cgctacgacg
     1321 ctcggcatcc tcgtcatgct gcccctcctg gcgatgaccg gcggcaccgt gctctacctg
     1381 cgcaaggtgg cacgggagac cggcaggctc gccaatcgct gtcctcgacc tcagggctgc
     1441 tcgaggcgga ccagttgctt cacagaaagg ccctcggcgc ctgggaccac cagacgcagc
     1501 ggtccttcgc tcgcgggcag cggcgccccg tcgacggtat gggccagcag cgcatctgtg
     1561 tgggcgagga tctcaaacgc cagggtctgc gtgtagccat cgctcgccac cgccgtcagc
     1621 gggcggtccg cggacgcctc gagcgcgctc accagcgccg gcagcgggac accggtgtaa
     1681 tgcttgccgt gatacgcgac actcgccgtc ggcatagcga cgagttcagc atgtgtccag
     1741 gtgcgcgccg atccggccac cgcaacctcg acgcccccca cgccgccgcc agtgtcggag
     1801 caggcgcacg cgagcagcag cgagagagcg agaggtcgta agccggtgag acgcatggcg
     1861 tcgccaccat acaccgtccg ccgcggtcag caagacgcca cgttgctcgc gtggggctcg
     1921 caaaggcgga ccatcttgcg ggaacggcaa gtttgctggc gcacccgact tcagggtgat
     1981 ctgagtaacg agcgcagccc ttcatccagg tcatcatcga agttcatggc gaacgaccag
     2041 gatcccctcg gcgcgaagcc tcaaacctgc gacgaccttg gtcgagatgg tttgcggcgc
     2101 gcgcctgatt tcctggcgtc gcttgcccgc gacgtgcgag gcccactggt cagcttggcg
     2161 acggccctgg cagtcttcga gcgctacgct cctcgggaca gcaacgagga acgcgcccgc
     2221 gaggttgctc gccgagcgac cgcagctctg ggccacatcg ctaatctcat cgatccgtgg
     2281 tctcacgtcg aggtgctacc gcctgttgtc gatgaaagcc cccgaagtcg cccgacccgc
     2341 gtgctgatca tcgaggacaa cgaggacctg cgcaccatcc tgaccgactt gtgccgtctg
     2401 ttgggccacg acgtatgcgc cgctggcgat ggtgttgacg gggtggccac ggcactccgt
     2461 caggtgcccg acattgcctt gatcgatatc ggcctccccg atatcgacgg ctgcgaagtg
     2521 gctcgccgca tacgcgagga cccgcgcggc gcgggcatcc aactcgtcgc catgaccgcc
     2581 tactcctccg ccgggcagcg cgcctcggca gcggccgctg gcttccgcct gtacgtcctg
     2641 aaatcggcca gtatcgagga actcgtcgcg gccctcgctg catgtacgtc atgaggttcg
     2701 aagctgcggc cgcaggcatg gtggcagacg aaagcgtcag ggcggcactg ccgcagtgag
     2761 ggccggcgcg cttcagccgc tgacttggcg tgcacccttc gaaagatggc aatcaaggac
     2821 ggctggtcgc gcgatgaccg cctgagcgcg
//
LOCUS       DASWNJ010000780         8451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_83861, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000780 DASWNJ010000000
VERSION     DASWNJ010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 8451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8451
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83861"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..5280
                     /locus_tag="VGB85_13060"
     CDS             160..5280
                     /locus_tag="VGB85_13060"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0135014.1"
                     /translation="MSKVGAIQFAGYDLPEVLHEEGEAVLLRCVRRDDGEPILAWMSL
                     EETPSRRLLERLQHEHAVLQHLAENIEERGEGRAAPLSLGLERDGAAVALMLEDPGGV
                     ALEQLLEGQALPVELAIELALAVTAALARVHAAGVQHRDLRPAHVLVDLARGQATLVG
                     FAAAVVADEAEAIQLPAASSAYMSPEMTGRTGRSSDMRSDYYSLGAMLYRMLTGRPPF
                     DESDPLRLIHAHIVRLPVAPEALRPTVPRGLSKVVLKLLAKTPEGRYQSAHGIKADLQ
                     RCRDDMRRGAPIDFEPGSADRSERLLLPDGLIGRGEQVAALLGVFERCGAGAPQVIVI
                     DGPTGVGKSSVLHELQTQIAARGGQIANGACRRLDQATPYAALAQGVQALVRQALAEP
                     GAPLQAWRERLLASLGGEGRLLFDVLPDLERVLGPQPPVVGLPPVERANRFRRVFRQL
                     LLGLARPQQPLVIVLEDVQWCDHATLQLLDDTLGGADTQHLMVFATMREGAPEAGSPL
                     AALLAAMAARKQPVHREKIRPLTREEVAAWLATALHPCLQDLSGLVDLVHASSAGNPF
                     LMRLLAESLHEQRALRFNGELGEWEWRPQQAAQARLPDDLGALVTDRLRELSPGAFSV
                     VTAAACVGARFDPRLLATLLQAPLAEVIAAVREAEARGLVIAVDGTYQFLHERVQQAA
                     SAALAPARAQALHLTIGRALASAGGGDFEVVHHLNLGAALLTDPNERRELAARNLAAA
                     QRALAATAVASAASYARAGAAALGPAAWEDAPGLTFALTRAEMEALYLLGEVEAGRAL
                     YQPLLRRAGDELERAALCELKVGLDIFHGRHHDAIASGVEGLARLGVELPAQPDAAAV
                     EARYLAVEAALAEHRLVRLIELPVARDPLAAAAMRILMAIGPAAMFVNGRLAFTISMA
                     IVDLTLRHGVTRATGYGFAGFGMYLAGARGQTARADALGQLALRLNARAGDDGLTAKI
                     HQLVGALIIGWVRPFAQARELLLQGYRLGVRTGDLMTACYNATSMVLVEVAHSASVGD
                     VRDSVAMLQRESGRVLAVYGSEVLVMASRWAGVLGGELPLPDASGNVGAWISSVFDVG
                     LENQSALIELYLNIYRTHVLYLFGCHAAVLPLPPRATERLSALATPMAVDALFFHALV
                     ACARIDAGKLGPDEREAALAEVTRAGERLLVWAIDCPENFSARHALLAAERARIVGRD
                     DEATGRYKDAIRAARRHGETGTEALACELASRHAAALGDDVLADMYLQTAATAYVRWG
                     ASAKAEQLARTHGLRTRRAVIERAAPDGDAFDLNSLIRAARALSGEIRLEQLLTTLLE
                     VAMENAGARRGIVVLAVDDGWRVEAEGDADPPAIRARMATPIEQHGGLPLSIIRYVAR
                     SQHDVLLARADREGEFRGDPYLAGGGVRSVLCTPVVHQGRCTGVLYLEHALSEGVFTA
                     QRLELIKQLAAQLAVSVENARLVEDLQRARNEAVAAERVKARFLLNMSHELRTPLNAV
                     IGYAELMQESLALGLHESLSSDLTRIRRAAYNLLRTLSGILELSKLASGASALESATV
                     EVAGLLREVAGEFHGLASSRGNVIELVCPPEIGSAYTDRSMLRYCLHSLVENACKFTR
                     DGRVIVKAERMQLDGRAHLLFSISDTGIGIPATHLETIFNAFTQVDDAANRVYEGTGV
                     GLTVTRQFGRMMGGEVSVVSEVGRGSTFTLRVPVSFVA"
     gene            5406..6017
                     /locus_tag="VGB85_13065"
     CDS             5406..6017
                     /locus_tag="VGB85_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135015.1"
                     /translation="MQYMTRNFILSLTAALPLFVTGACGDNGGGTEGASSSSTGGATS
                     TTGDGTTTAPTSSTTEPATTEPATSSTTEPATSSTTEPATTTTTGDSTTDVTATTGEP
                     DTSSGSTGDPLAECLMMVEPGDMCGECACKSCTAEYEDCQSDMGCAKIFKCVQDSGCS
                     GQDCALPCGQTIQENGGVMGEPLAKAITLGMCIGNNCMAECEG"
     gene            complement(6061..8271)
                     /locus_tag="VGB85_13070"
     CDS             complement(6061..8271)
                     /locus_tag="VGB85_13070"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY0135016.1"
                     /translation="MLRPSHYVLLALAAACSGEPAADATTDTGDVETDTSTTDAGTGD
                     PPEPTPPMLPPRPAEQSCRFDGQAPGVLPPLAEVPVFVDAALDEVVDVVPAGPDGRVL
                     VAERGGRIVAGDVDAGALAPVVDLKARGRIVALAPAPDFVTSGHLFVRLEASVPARAV
                     VLRLTVDLATWTADPASERVVIEVADAVGERSGGALTFDGGGMLIIGIGDLGEQVTAA
                     AQDPTDIRGKLLRIDVAPLEATNTYAHPADNPFVGEGGDADVVWAVGLRDPWRCSPAE
                     AGALWCVDVGESEHEVDHVSAGADLGWPYIEGGTCRLPGGDCSGLNADWPAANYRSGD
                     GDCGLAGALFGVSPGLETSLVYADRCSGRLRGIDTADPGVVVQEELLAAEPAGIVTLA
                     RDGRGAPIAVTGDDRLVRLEVAPPPGAFPTRLSTSGCFTDLAALAPVPGVVPYGVNAE
                     LWSDGAIKSRFIALPPTATITVADDGSFVFPTGTVLLKTFTFEFIAGDPGSRRPVETR
                     VMVKREHGWQFHSYQWDADGRDATLLLDDAEQTLVIAEQDGTIELDYAWPSRGTCKVC
                     HGFGESRALGPAIAQLNGDHDYGDGTANQLTILSGLGMFAAPLPGPPATLPRIPARDE
                     QEASLELRARGYLHTNCGHCHRPDGWTPPDLTMDLRWSTPLVDTRICDVPTQYDNPWT
                     TGELRVAAGDPESSVIWQRIGQRGPGQMPLLGTNRVDPGATVVRDWIAQLSACP"
BASE COUNT         1111 a         3103 c         3099 g         1138 t
ORIGIN      
        1 gtcctcgtcg ctcgacgcgc gcttcgcagc gaggtctgca cgtgcaacta cgaagattgc
       61 cggcggacgt cgcggacccg ggtcgcgacg cgcgagaaca cgggcctgcc gccgtcgagg
      121 ccatgctagg gtgcacctgc cctctgcgag cctgcccgca tgtctaaagt cggcgcgata
      181 caattcgcgg ggtacgacct gccggaggtg ctccatgagg agggcgaggc cgtgctgctg
      241 cgctgcgtcc ggcgcgacga cggcgagccg atcctcgcgt ggatgtcgct cgaggagacg
      301 ccgagccgcc gtctgctcga gcgtctgcaa cacgagcacg cggtgctgca gcacctggcc
      361 gagaacatcg aggagcgcgg cgagggccgg gcggctccgc tgtcgctcgg gctcgagcgc
      421 gacggcgccg cggtcgcgtt gatgctcgag gaccccggcg gggtcgcgct cgagcagctg
      481 ctcgagggtc aggccttgcc ggtcgagctg gcgatcgagc tcgcgctggc ggtcacggcc
      541 gcgctggccc gcgtgcacgc ggccggggtc cagcaccgcg acctgcggcc ggcgcacgtg
      601 ctcgtcgatc tcgcgcgcgg acaggcgacg ctggtcggct tcgcggcggc ggtggtcgcc
      661 gacgaggcgg aggcgatcca attgccagcc gcatcgtcgg cttacatgtc acccgagatg
      721 accggacgga ccggccgcag cagcgacatg cgcagcgact actactcgct cggcgcgatg
      781 ctgtatcgca tgttgaccgg tcgcccgccg ttcgacgaga gcgacccgct acggctgatc
      841 cacgcgcaca tcgtgcgatt gccggtcgcc ccggaggcgc tgcgcccgac ggtcccgcgc
      901 gggctctcga aggtcgtgct caagctgctc gccaagaccc ccgaggggcg ctaccagagc
      961 gcgcacggga tcaaggccga cctgcaacgc tgccgcgacg acatgcgacg cggcgcgccg
     1021 atcgacttcg aacctggctc tgcggacagg tcggagcggc tgctcctgcc cgacgggctg
     1081 atcgggcgcg gcgagcaggt cgccgcgttg ctcggggtgt tcgagcgctg cggcgccggg
     1141 gccccgcagg tgatcgtgat cgacgggccg accggcgtcg gcaagtcgtc ggtgctgcac
     1201 gagctccaga cccagatcgc agcgcgtggc ggacagatcg ccaacggcgc gtgccggcgg
     1261 ctcgaccagg cgacccccta cgccgcgctc gcgcagggtg tgcaggcgct ggtgcgccag
     1321 gcgctcgccg agcccggcgc gccgctgcaa gcctggcgcg agcggctgct cgcgtcgctc
     1381 ggcggcgagg gccggctgct gttcgacgtg ctaccggacc tcgagcgcgt gctcgggccg
     1441 cagccgccgg tggtcggctt gccgccggtc gagcgggcca accgctttcg tcgcgtgttt
     1501 cgccagctgt tgctgggcct cgcgcgcccg cagcagccgc tggtgatcgt gctcgaggac
     1561 gtccagtggt gtgaccacgc gacgctgcaa ttgctggacg acacgctcgg cggggcggac
     1621 acgcagcacc tgatggtgtt cgcgacgatg cgcgaggggg cgcccgaggc cggcagcccg
     1681 ctcgcggcgc tgctcgccgc gatggcggcc cgcaaacagc cggtccaccg cgagaagatc
     1741 cggccgctga cccgcgagga ggtcgcggcc tggctggcca ccgcgctgca cccgtgcctg
     1801 caggacctct ccgggctcgt cgacctcgtc cacgccagca gcgccggcaa cccgttcctc
     1861 atgcgcctgc tcgccgagtc gttgcacgag caacgcgcgc tgcgcttcaa cggcgagctc
     1921 ggcgagtggg agtggcgacc ccagcaggcc gcgcaggccc gcctgcccga cgacctcggc
     1981 gcgctcgtca ccgaccgcct gcgcgagctg tcgccgggcg ccttctcggt cgtcaccgcg
     2041 gcggcctgcg tcggcgcgcg cttcgacccg cgcctgctgg cgacgctgct gcaagcgccg
     2101 ctcgccgagg tgatcgcggc ggtgcgcgag gccgaggccc gcggcctggt gatcgcggtc
     2161 gacggcacct atcagttcct gcacgagcgc gtgcagcagg ccgcgagcgc cgcgctggcg
     2221 cccgcgcggg cccaggcgct gcacctgacg atcgggcgcg cgctcgccag cgccggcggc
     2281 ggtgacttcg aggtcgtcca tcacctcaac ctcggcgccg cgctgctgac cgaccccaac
     2341 gagcgccgcg agctggccgc gcgcaacctc gccgcggccc agcgcgccct cgcggcgacc
     2401 gccgtcgcct cggccgccag ctacgcgcgg gccggcgccg ccgcgctcgg gcccgccgcg
     2461 tgggaggatg cgccgggcct gacttttgcg ctgacgcggg ccgagatgga ggcgttatac
     2521 ctgctcgggg aggtcgaggc cgggcgggcc ctgtatcagc cgctgctgcg gcgcgccggc
     2581 gacgagctcg agcgcgccgc gctgtgtgag ctcaaggtcg ggctcgacat cttccacggc
     2641 cgccaccacg acgcgatcgc ttccggcgtc gagggcctcg cgcgcctcgg cgtcgagctg
     2701 ccggcgcagc cggacgccgc cgcggtcgag gcgcgctacc tcgcggtcga ggcggcgctc
     2761 gccgagcatc gcctggtccg cctgatcgag ctgccggtcg cgcgcgaccc gctggccgcg
     2821 gcggcgatgc ggatcctgat ggcgatcggg ccggcggcga tgttcgtcaa cgggcgcctg
     2881 gcgttcacga tctcgatggc gatcgtcgac ctcacgctgc gtcacggcgt cacccgggcg
     2941 accggctacg gcttcgccgg cttcggcatg tacctcgcgg gcgcgcgcgg acagaccgcc
     3001 cgcgccgacg cgctcggcca gctcgcgctg cgcctcaacg cccgcgccgg cgacgacggc
     3061 ctgaccgcga agatccacca gctcgtcggc gcgctgatca tcggctgggt ccgacccttc
     3121 gcccaggcgc gcgagctgct gctgcaaggc tatcgcctcg gcgtgcgcac cggcgacctg
     3181 atgaccgcct gctacaacgc gaccagcatg gtgctcgtcg aggtcgccca cagcgccagc
     3241 gtcggcgacg tgcgcgacag cgtggcgatg ctgcagcgcg agtcgggccg cgtgctcgcg
     3301 gtctacggca gcgaggtgct ggtgatggcg agccgctggg cgggcgtgct cggcggcgag
     3361 ctgccgttac cggacgcgtc cgggaacgtc ggcgcgtgga tctcgagcgt gttcgacgtc
     3421 ggcctcgaga accagtcggc gctgatcgag ctgtacctca acatctatcg cacgcacgtg
     3481 ctctacctgt tcggctgtca cgcggccgtg ctgccgctgc cgccgcgggc gaccgagcgg
     3541 ctctcggccc tcgccacgcc gatggcggtc gacgccctgt tcttccacgc gctcgtcgcc
     3601 tgcgcgcgca tcgacgccgg caagctcggc cccgacgagc gcgaggcggc gctcgccgag
     3661 gtcacgcggg ccggcgagcg cctgctcgtc tgggcgatcg actgcccgga gaacttctcg
     3721 gcccgccacg ccctgctcgc ggccgagcgc gcgcgcatcg tcgggcgcga cgacgaggcg
     3781 accggccgct acaaggacgc gatccgggcc gcccgccgcc acggcgagac cggcaccgag
     3841 gcgctcgcct gcgagctcgc cagccgccac gcggcggccc tcggcgatga tgtcctcgcg
     3901 gacatgtact tgcagacagc cgcgaccgcg tacgtccgct ggggcgcgtc ggccaaggcg
     3961 gagcagctcg cgcggaccca cggcctgcgc acgcggcggg cggtgatcga gcgcgccgcg
     4021 cccgacggcg acgccttcga cctcaacagc ctgatccggg ccgcgcgcgc gctgtcgggc
     4081 gagatccggc tcgagcagct gttgacgacg ctgctcgagg tcgcgatgga gaacgccggg
     4141 gcgcggcgcg gcatcgtcgt gctcgcggtc gacgacggct ggcgcgtcga ggccgagggc
     4201 gacgccgacc cgccggcgat ccgcgcccgc atggcgacgc cgatcgagca gcacggcggg
     4261 ctgccgctgt cgatcattcg ctacgtcgcg cgcagccagc acgacgtgct gctggcccgc
     4321 gccgaccgtg agggggagtt ccgcggcgac ccatacctcg ccggcggcgg cgtgcgctcg
     4381 gtgctgtgta cgccggtcgt gcatcagggt cgctgcaccg gcgtgctgta cctcgagcac
     4441 gcgctgtcgg agggcgtgtt cacggcgcag cggctcgagc tgatcaagca gctcgcggcg
     4501 cagctggcgg tgtccgtcga gaacgcgcgg ctggtcgagg acctgcagcg ggcccgcaac
     4561 gaggccgtcg cggccgagcg ggtcaaggcc cgcttcctgc tcaacatgag ccacgagctg
     4621 cgcaccccgc tcaacgccgt gatcggctac gccgagctga tgcaggagag cctcgcgctc
     4681 ggcctccacg agtcgctcag ctccgacctg acgcggatcc gtcgcgccgc gtacaacttg
     4741 ttgcgcacgc tcagcggcat cctcgagctg tcgaagctgg cgagcggcgc ctcggcgctc
     4801 gagagcgcga cggtcgaggt cgccggcctg ctgcgcgagg tcgccggtga gttccacggg
     4861 ctcgccagct cgcgcggcaa cgtgatcgag ctcgtgtgtc cgccggagat cggcagcgcc
     4921 tacaccgacc gctcgatgct tcgctactgt ctacacagcc tggtcgagaa cgcctgcaag
     4981 ttcacgcgcg acggtcgcgt catcgtcaag gccgagcgca tgcagctcga cggccgcgcg
     5041 cacctgctgt tctcgatctc cgacaccggc atcggcatcc cggcgaccca cctcgagacg
     5101 atcttcaacg ccttcaccca ggtcgacgac gccgccaacc gcgtgtacga gggcaccggc
     5161 gtcggcctca ccgtcacgcg ccagttcggc cgcatgatgg gcggtgaggt cagcgtcgtc
     5221 agcgaggtcg gccgtggctc caccttcacg ctgcgcgtcc ccgtcagctt cgtcgcctga
     5281 gcgctcgcag cgttcaccgc gccgcgccgc ggtctccatt tgatttcgcg gcctcgcacc
     5341 cccagcggag ccccgagatt cgtctccggt ttgggcgcga gcggccgacc atgtaccgtt
     5401 gtttcatgca gtacatgacc cgtaacttca tcctctccct gaccgccgcg ctcccgctgt
     5461 tcgtcaccgg tgcctgcggc gacaacggcg gcggcaccga gggcgcctcg agcagcagca
     5521 ccggcggcgc cacctcgacg accggcgacg gcacgaccac cgcgccgacc tcgtcgacga
     5581 ccgagccggc gacgacggag ccggcgacct cgtcgacgac ggagccggcg acctcgtcga
     5641 cgacggaacc cgcgacgacg acgacgaccg gcgactcgac gaccgacgtc accgcgacga
     5701 ccggcgagcc cgacacgagc agcggcagca ccggcgaccc gctggcggag tgcctgatga
     5761 tggtcgagcc gggcgacatg tgcggcgagt gtgcctgcaa gagctgcacg gcggagtacg
     5821 aggactgcca gtcggacatg ggctgcgcga agatcttcaa gtgcgtccag gactccggct
     5881 gcagcggtca ggactgcgcc ctgccgtgcg gccagacgat ccaggagaac ggcggcgtca
     5941 tgggtgagcc gctcgccaag gcgatcacgc tcggcatgtg catcggcaac aactgcatgg
     6001 ccgagtgcga gggctgatct cgctgcctgg cgaggaacgg ctccgtgacg ccgttcctcg
     6061 tcacgggcac gcggacagct gcgcgatcca gtcgcgcacg acggtcgcgc cggggtcgac
     6121 gcggttggtg ccgagcaggg gcatctggcc gggcccgcgc tggccgatcc gctgccagat
     6181 gaccgaggac tcgggatcac cggcggcgac ccgcagctcg ccggtggtcc acgggttgtc
     6241 gtactgcgtc ggcacgtcgc agatgcgcgt gtccaccaaa ggcgtgctcc accgcaggtc
     6301 catcgtcagg tccggcggtg tccagccgtc cgggcggtgg cagtgcccgc agttggtgtg
     6361 caggtagccg cgggcgcgca gctcgaggct ggcctcctgc tcgtcgcggg cggggatccg
     6421 cggcagcgtc gcgggcgggc cgggcagcgg cgcggcgaac atgccgagcc cggagaggat
     6481 cgtcagctga ttcgcggtgc cgtcgccgta gtcgtgatca ccgttgagct gggcgatcgc
     6541 cgggccgagc gcgcgcgact cgccgaagcc gtggcacacc ttgcaggtgc cgcggctcgg
     6601 ccacgcgtag tcgagctcga tcgtcccgtc ctgctcggcg atcacgagcg tctgctcggc
     6661 gtcgtcgagc agcagcgtcg cgtcgcggcc gtcggcgtcc cactgatacg agtgaaactg
     6721 ccagccgtgc tcgcgcttga ccatcacgcg cgtctcgacc ggccggcgcg agccggggtc
     6781 gccggcgata aattcgaagg tgaaggtctt gagcagcacg gtgccggtcg ggaatacgaa
     6841 gctgccgtcg tcggcgacgg tgatcgtcgc cgtcgggggc agggcgatga acctcgactt
     6901 gatcgcgccg tccgaccaca gctcggcgtt gacgccgtag ggcaccacgc cgggcaccgg
     6961 cgcgagcgcg gcgaggtcgg tgaagcagcc gctggtggac agccgcgtcg ggaacgcgcc
     7021 cggcggcggc gcgacctcga ggcgcacgag tcggtcgtcg ccggtcacgg cgatcggtgc
     7081 gccccggccg tcgcgcgcga gggtgacgat gccggcgggc tccgccgcga gcagctcctc
     7141 ctgcacgacg acgccggggt ccgcggtgtc gatgccacgc aggcggcccg agcagcggtc
     7201 ggcgtacacg aggctggtct cgaggcccgg ggacacgccg aacagcgcgc ccgcgaggcc
     7261 gcagtcgccg tcgccgctgc gatagttggc ggccggccag tccgcgttga ggccgctgca
     7321 gtcgccgccg ggcagccggc aggtgccgcc ctcgatgtac ggccacccga ggtcggcccc
     7381 ggcgctgaca tggtcgacct cgtgctcgga ctcgccgaca tcgacacacc acagcgcgcc
     7441 ggcctcggcg ggcgagcacc gccaggggtc acgcagcccg acggcccaca ccacgtccgc
     7501 gtcgccgccc tcgccgacga aggggttgtc cgcggggtgc gcataggtgt tggtggcctc
     7561 gagcggggcg acgtcgatgc gcagcagctt gccgcggatg tcggtcgggt cctgcgcggc
     7621 cgcggtgacc tgctcgccga ggtcaccgat gccgatgatc agcatgccgc cgccgtcgaa
     7681 ggtcagcgcg ccgccgctgc gctcgccgac cgcgtcggcg acctcgatca cgacgcgctc
     7741 gctcgccgga tcggcggtcc aggtcgcgag gtcgacggtg aggcggagga ccacggcgcg
     7801 ggccggcacg ctcgcctcga ggcggacgaa caggtgcccg ctcgtcacga agtccggcgc
     7861 aggggcgagc gcgacgatcc gcccgcgcgc cttcagatcg acgaccggcg ccagcgcgcc
     7921 ggcatcgaca tcacccgcga cgatcctgcc cccgcgctcg gccaccagca cgcggccgtc
     7981 cgggcccgcg gggacgacgt cgacgacctc gtcgagcgcc gcgtcgacga agaccggcac
     8041 ctccgcgagc ggcggcagca cgcccggcgc ctgaccgtcg aagcggcagc tctgctcggc
     8101 gggccgcggc ggcaacatcg gcggcgtcgg ctccggcgga tctccggtgc ctgcatccgt
     8161 ggtgctcgtg tcggtctcga cgtcaccggt gtccgtggtc gcgtccgcgg ccggctcacc
     8221 gctgcacgcc gcggcgagcg cgaggagcac gtagtgcgag ggacgcagca tcgacgccgc
     8281 gagccaaccg cgaacttcgt cgcgtcgcaa gcacattcgc agatgcgccg cgtccgcccg
     8341 cgtccgggcg cgagtctgcc cgccggtcgc gctactgcgc ggcggccttg agcgcgtcgg
     8401 cgatcgcggc ctcctgggcg tccatcgtcg tcagcagcga gttgcagggc a
//
LOCUS       DASWNJ010000781         1576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_262489, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000781 DASWNJ010000000
VERSION     DASWNJ010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262489"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VGB85_13075"
     CDS             <1..312
                     /locus_tag="VGB85_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135017.1"
                     /translation="RLHQLATRMLWYNHALLPARGQGAPLRPEDEAQLATMVRGLART
                     ARLDRALRHAWLCVAGSVAVLTPTLGLVAVAMLLAVVVAMVAAVQLAGLFRPDVSAGR
                     G"
     gene            complement(331..>1576)
                     /locus_tag="VGB85_13080"
     CDS             complement(331..>1576)
                     /locus_tag="VGB85_13080"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY0135018.1"
                     /translation="PQRDVPKIYWAMLLMLLVVGGSLAILVFLLLPPHVLLEHSGDLL
                     GQLGRHVAATLTGSAGTGDIWHAVVVANAALMLIAATNTAFVGARGLWLAMARDDLLP
                     RVLLTPNQRGEFSRLHGMMLLACFLLAIQADWHVGTLERWYWAMFGLVLFSGMIGFVL
                     LRRFKAEDRRVYTAPWNITLFGTRLPVGALVGGAALTYVLLTMWTNSTAPMGELRTLF
                     TTVVVLVGLVVLVHNHRPLIRAGYRYFRRVLETVESTVIDPQQRTIVVAVGGVRVGRL
                     IQKAVELARQQSRATGIPYRQVVVFHMTKSVRSEFVYRVTRDAIRPEGIEGGVIRIFT
                     ELTEIAPTDMDLYLALVPNKHAHKSPLYAALDALVAFHERHSFKGHMIMIGTYGVKPA
                     DIEELQGRLKNTILVPVPLFDD"
BASE COUNT          239 a          516 c          600 g          221 t
ORIGIN      
        1 cgcctgcatc agctggcgac gcgcatgctc tggtacaacc acgcgctgct gccggcgcgc
       61 ggccaggggg cgccgctgcg gcccgaggac gaggcccagc tggcgacgat ggtccgcggg
      121 ctcgcccgca cggcccgcct cgatcgcgcg ctgcggcacg cctggctgtg cgtggccggc
      181 agcgtcgccg tgctgacgcc gacgctcggg ctcgtcgcgg tggcgatgct gctggcggtg
      241 gtcgtcgcca tggtcgcggc ggtgcagctc gccgggctgt ttcgcccgga tgtctcggcc
      301 gggcgcgggt gagccggatc gccgacgccc tcagtcgtcg aacagcggga ccggcacgag
      361 gatcgtgttc ttgagccgcc cctgcagctc ctcgatgtcg gccggcttca cgccgtaagt
      421 gccgatcatg atcatgtggc ccttgaagct gtgccgctcg tgaaacgcga cgagcgcgtc
      481 gagggcggcg tagagcgggc tcttgtgcgc gtgcttgttc ggcacgagcg cgagatacag
      541 atccatgtcg gtcggggcga tctcggtcag ctcggtgaag atgcggatca cgccgccctc
      601 gatgccctcc gggcggatcg cgtcgcgggt gacgcggtac acgaactcgc tgcgcacgct
      661 cttggtcatg tggaacacga cgacctggcg gtacgggatg ccggtcgcgc ggctctgctg
      721 gcgggcgagc tcgacggcct tctggatcag ccggccgacc cgcaccccgc cgacggcgac
      781 gacgatcgtg cgctgctggg gatcgatgac ggtcgactcg acggtctcga gcacgcgacg
      841 aaagtagcgg tagccggcgc ggatcagcgg gcggtggttg tgcacgagca cgacgaggcc
      901 gacgagcacg acgacggtgg tgaacagcgt ccgtagctcg cccatcggcg cggtgctgtt
      961 ggtccacatc gtcagcagca cgtaggtgag cgcggcgccg ccgacgagcg cgccgaccgg
     1021 gaggcgggtg ccgaacaggg tgatgttcca cggcgcggtg tagacgcggc ggtcctcggc
     1081 cttgaagcgg cgcagcagca cgaagccgat catccccgag aacaggacca ggccgaacat
     1141 cgcccagtac cagcgctcga gcgtgccgac gtgccagtcg gcctggatcg ccagcaggaa
     1201 gcaggcgagc agcatcatgc cgtgcaggcg cgagaactcg ccgcgctggt tcggggtcag
     1261 cagcacgcgc gggagcaggt cgtcgcgcgc catcgccagc cacagaccgc gcgcgccgac
     1321 gaacgcggtg ttggtcgcgg cgatcagcat cagcgccgcg ttggcgacga cgacggcgtg
     1381 ccagatgtcg ccggtgccgg ccgagccggt cagcgtggcg gcgacgtggc ggccgagctg
     1441 gccgagcagg tcgccggagt gctcgagcag cacgtgcggg ggcaggagta aaaatacgag
     1501 gatcgccagc gagccgccga cgacgagcag catcaggagc atcgcccagt agatcttggg
     1561 gacgtcgcgc tgcggg
//
LOCUS       DASWNJ010000782         2595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_262531, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000782 DASWNJ010000000
VERSION     DASWNJ010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262531"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /gene="argB"
                     /locus_tag="VGB85_13085"
     CDS             <1..461
                     /gene="argB"
                     /locus_tag="VGB85_13085"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEY0135019.1"
                     /translation="SAGPDPIDFGLVGDIVEIRTELLRHLWAGGYTPVVNTLGIDPAK
                     GEVYNINADTVASAIAAALKVDHLFLMTGVPGVLQNKDDPSTRIPRLTAAEARQAIAS
                     GVIVGGMIPKVEEALANIELGIGAVHILGATAGALQAEAQQPGSRGTVLV"
     gene            482..1717
                     /locus_tag="VGB85_13090"
     CDS             482..1717
                     /locus_tag="VGB85_13090"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0135020.1"
                     /translation="MHTPRNTELGTKLQAAVDEAFEATQVAWLRRVVEQPSHTYARAD
                     VEAAAAIVDELAAEVGLTRTLHPDPSGKFADHRVYATAATGADDRALALVGHIDTVFP
                     RSLGFLEFRREGDSVFGPGVLDMKSGVSSTLFALRALRHVAPEVHASLRARVVIVSDE
                     EVGSPSSHALYDHLAPRTTAGLVFESGRKQDLIVTARKGGGVYTLTVHGKAAHAGNDH
                     KSGVNAIHALALIVGRIEAMTNYGRGVTLNVGLIEGGTAKNTVPERASCQIDARFETV
                     SDAEHVGAALQAITREPFAGIDPALVPERLRQVRVELSGGVTRPPMEPIAASQRLRLA
                     YEPYAGALGLGVGEAKLQGGGSDANLLVAAGVPCIDGLGPYGEFFHQVSEWCSLTSLR
                     RRTAALAWFLAEQAANDYA"
     gene            1935..>2595
                     /locus_tag="VGB85_13095"
     CDS             1935..>2595
                     /locus_tag="VGB85_13095"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF21 domain-containing protein"
                     /protein_id="HEY0135021.1"
                     /translation="MFFSGSETAITSFDAHRAQRMVEEDGRSGKVMAFWVRDPVRVLS
                     TILVGNNIANTLLGAVITAMAIRHLEGSPWGDYAIGAAVFVATALLLVIGEITPKALG
                     KLFSDRFTVPALWVLKLVSRIIAPILWIVMPLTNRLIRRAAPDANASASLARVTSDDI
                     SYMIKVGHRDGSIAADQAALLSSMVRFEAKIARDIMIPENRVTAVNLAWDVRQVREVA
                     LR"
BASE COUNT          380 a          963 c          895 g          357 t
ORIGIN      
        1 ccagcgccgg cccggacccg atcgacttcg gcctcgtcgg cgacatcgtc gagatccgca
       61 cggagctgct gcgccacctg tgggccggcg ggtacacgcc ggtcgtcaac accctcggga
      121 tcgatcccgc gaagggcgag gtctacaaca tcaacgccga caccgtcgcc tcggcgatcg
      181 ccgcggcgct caaggtcgat catttgttcc tgatgaccgg cgtgcccggc gtgctgcaga
      241 acaaggacga cccgtcgacc cggatcccgc ggttgaccgc cgccgaggcg cgccaggcga
      301 tcgcctccgg ggtgatcgtc ggcggcatga tccccaaggt cgaggaggcg ctcgcgaaca
      361 tcgagctcgg catcggcgcg gtgcacatcc tcggggccac cgccggcgcg ttgcaggccg
      421 aggcgcagca gccgggcagc cgcggcaccg tgctcgtgta gggctgctat agtcgcgggc
      481 gatgcacacc ccgcgcaaca ccgagctcgg cacgaagctc caggccgccg tcgacgaggc
      541 cttcgaggcg acgcaggtcg cgtggctgcg ccgcgtcgtc gagcagccga gccacaccta
      601 cgcgcgcgcc gacgtcgagg ccgcggcggc gatcgtcgac gagctcgccg ccgaggtcgg
      661 cctgacccgc acgctgcacc ccgacccctc cggcaagttc gcggaccacc gcgtgtatgc
      721 caccgcggcg accggcgccg acgaccgggc cctcgcgctc gtcggccaca tcgacaccgt
      781 gttcccgcga tcgctcggct tcctcgagtt tcgccgcgag ggcgactcgg tgttcggccc
      841 cggcgtgctc gacatgaagt ccggcgtctc gtcgacgctg ttcgccctgc gcgcgctgcg
      901 acacgtcgcc ccggaggtgc acgcgagcct gcgcgcgcgc gtcgtgatcg tcagcgacga
      961 ggaggtcggc tcgccgagca gccacgcgct gtacgaccac ctcgcgccgc gcacgaccgc
     1021 cggcctggtg ttcgagtcgg ggcgcaagca ggacctgatc gtcacggcgc gcaagggcgg
     1081 cggcgtgtac acgctgacgg tccacggcaa ggccgcccac gccggcaacg accacaagtc
     1141 cggcgtcaac gcgatccacg cgctcgcgtt gatcgtcggg cgcatcgagg cgatgacgaa
     1201 ctacggccgc ggcgtcaccc tcaacgtcgg gttgattgaa ggcggcaccg ccaagaacac
     1261 cgtgcccgag cgcgcgtcgt gccagatcga cgcccgcttc gagaccgtca gcgacgccga
     1321 gcatgtcggc gcggcgctgc aggcgatcac acgcgagccc ttcgccggca tcgaccccgc
     1381 cctcgtgccc gagcgcctgc gtcaggtccg ggtcgagctg tccggcggcg tgacccgccc
     1441 gccgatggag ccgatcgccg cctcgcagcg cctgcgcctc gcctacgagc cctacgccgg
     1501 cgcgctcggg ctcggggtcg gcgaggccaa gctgcaaggc ggcggctccg acgccaacct
     1561 gctggtcgct gcgggagtac cctgcatcga cggcctcgga ccctacggcg agttctttca
     1621 tcaggtcagc gagtggtgca gcctgaccag cctgcgccgc cggaccgccg cgctcgcgtg
     1681 gttcctcgcc gagcaggccg ccaacgacta cgcgtgaacc gcggagggct gtccgcacgc
     1741 cggcgacacg gccgggtacg gacaccccca tggcggcgta acagcgcagc agcggccccc
     1801 gggagcccgt tgggccgtgg gggcgggcgg cggcttgcga gccccgggca cccgtgcgaa
     1861 aacggtcagg tccctcgatg agtgacgccg acgccctggg actcctgctt gtcgtgctgt
     1921 gcctgaccgc cagcatgttc ttttccggtt ccgagacggc gattaccagc ttcgacgccc
     1981 accgggcgca gcggatggtc gaggaggacg ggcgcagcgg caaggtgatg gccttctggg
     2041 tgcgcgaccc ggtgcgggtg ctgtcgacga tcctggtcgg caacaacatc gccaacacgc
     2101 tgctcggcgc cgtgatcacg gcgatggcga tccgccacct cgagggcagc ccgtggggtg
     2161 actacgcgat cggggcggcc gtgttcgtgg cgaccgcgct gctgctcgtc atcggcgaga
     2221 tcacgcccaa ggcgctcggc aagctgttca gcgaccgttt caccgtcccg gcgctgtggg
     2281 tgctcaagct cgtcagccgg atcatcgcgc cgatcctgtg gatcgtgatg ccgctgacca
     2341 accgcctgat ccggcgcgcc gccccggacg ccaacgcgag cgccagcctc gcgcgcgtca
     2401 cctccgacga catcagctac atgatcaagg tcggccaccg ggacggatcg atcgccgccg
     2461 accaggccgc gctgctgtcg agcatggtcc gcttcgaggc gaagatcgcc cgcgacatca
     2521 tgatccccga gaaccgcgtc accgcggtca acctcgcctg ggacgtccgc caggtccgcg
     2581 aggtcgcgct gcggt
//
LOCUS       DASWNJ010000783         3028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_50103, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000783 DASWNJ010000000
VERSION     DASWNJ010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50103"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..962)
                     /locus_tag="VGB85_13100"
     CDS             complement(159..962)
                     /locus_tag="VGB85_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135022.1"
                     /translation="MRIVEIGSSPAHEIEYLDAAPGGGSQVRRLPVLRGVVEGLPAEL
                     DALLIAADLQGRARARDGELRLLGEALAEDYAALAELEQVPAPAFTGVVLAGDLFAAP
                     GADRMGATGDVRSVWRAFAGAFRWVTGVAGNHDLFGGKQEHSRFTREAGVHLLDDESV
                     VLDGLRIAGVGGICGNPNKPNRRAPEEFLAAVRRGLGRKPHMLVLHEGPDGDDPRLRG
                     NSEIRAAVAASRAIVICGHVHWDRPLVTLPGGAQLLNVDARAVLLERAQ"
     gene            1068..1928
                     /locus_tag="VGB85_13105"
     CDS             1068..1928
                     /locus_tag="VGB85_13105"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0135023.1"
                     /translation="MSAPHARACPACGFDTARFAFETAGYAHVRCERCDTLFVAELPS
                     AERVEATYLAPDYHSSAKGQEQRMRAEADARAQVLRKFGCRDILEIGCGPGHFLDAVR
                     DLGMQIEGVDRAPSTEAARERGHLVHEVWLQDLGPVTPRFDAVAMWEVIEHVGDPAAV
                     LQRVRQWLRPGGFLALSTPSSSGIPARVLGSRFPMVCPPEHLTLFSRKGLAALLGAAH
                     FDPVRWTSFSGLGREQLRRGFQRFLLGGSLPGRAVASGLAAVAEVPVQLMDRAGLGSG
                     FEVYSVATPD"
     gene            complement(1938..>3028)
                     /locus_tag="VGB85_13110"
     CDS             complement(1938..>3028)
                     /locus_tag="VGB85_13110"
                     /inference="COORDINATES: protein motif:HMM:NF015254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pectin acetylesterase-family hydrolase"
                     /protein_id="HEY0135024.1"
                     /translation="SSGEGPALAEPEDPELPLPEFDGEPIPDAPVGLWQWVDFPDSRC
                     RDGSSTGIGVRRGSTDKLVIFFEGGGACFNGFTCLANLGSFGLPDFNDWQYGPGFGGI
                     FDSSHEDNPVRDWSFVYVPYCTGDVHAGSNSDIEIADVQGVQQFHGYTNVEQFLKRIV
                     PSFGDSSHVLVTGVSAGGFGAAFNYHRIARIFSGKVTLLDDSGPAMRDPYLAPCLQQM
                     WRDAWGLDDTIPLTCKDCWAEDGGSISELVRWIGTRHSQQRMGLISSERDRTISTFFS
                     FGLNECAGLGAIYPSNRFAEGIHDFVDAMLKPTGNWSTYIIPGVDHAWIGHWAFYNTE
                     VDGVRLVDWVSELLDGEVTHVAPNVTGA"
BASE COUNT          445 a         1111 c         1023 g          449 t
ORIGIN      
        1 ctcttacacc cgcagcagat cgcacagcgt gggctgttcg acccggccga ggtcgcccgc
       61 ctgctcgccg agcaccgcct cggccagcgc gaccacagcc accgcatctg gtcgctgctg
      121 gtgctcgagc agtggcagcg catgtacgac gcctgatact actgcgcgcg ctcgagcagc
      181 accgcccgcg cgtcgacgtt gagcagctgc gccccgccgg gcagcgtcac cagcggcctg
      241 tcccagtgga catgtccgca gattacaatt gccctgctcg cagccaccgc cgcgcggatc
      301 tcggagttgc cacgcagccg cgggtcgtcg ccgtcgggcc cctcgtgcaa caccagcatg
      361 tgcggcttgc gaccgaggcc gcgacgcacc gccgcgagga actcctccgg cgcgcgacgg
      421 ttcggcttgt tcgggttgcc gcagatccct ccgaccccgg cgatacgcag gccgtcgagc
      481 accacggact cgtcgtcgag caggtgcacg ccggcctcgc gggtgaagcg gctgtgctcc
      541 tgcttgccgc cgaacaggtc gtggttgccc gcgaccccgg tgacccagcg aaacgctccc
      601 gcgaaggcgc gccacaccga gcgcacgtcg ccggtcgcgc ccatgcggtc ggccccgggc
      661 gcggcgaaca ggtcgccggc gagcacgacc ccggtgaacg cgggcgcggg gacctgctcg
      721 agctcggcga gcgcggcata gtcctctgcg agcgcctcgc cgagcagccg cagctcgccg
      781 tcccgggccc gcgcgcggcc ctgcaggtcc gccgcgatca gcagcgcgtc gagctcggcc
      841 ggcaggccct cgacgacgcc gcgcaggacc ggcagccggc gcacctggga acctcccccg
      901 ggggcagcgt cgaggtactc gatctcgtgg gccggcgacg acccgatctc gacgatccgc
      961 atgaccgacc gagtatgcca cccgggtcgc gcggtggatc gcagtggaac gcgtgcatcc
     1021 gcgccgtgaa ctggcgcggc ccgccgtcga cgtggtaccc ctgtctcatg tcggcaccgc
     1081 acgcgcgcgc ttgtccggcc tgtggcttcg atacggcgcg cttcgccttc gagaccgccg
     1141 gctacgccca cgtccgttgc gagcgctgcg acacgttatt cgtcgccgag ctgccgtcgg
     1201 ccgagcgcgt cgaggcgacc tacctcgcgc ccgactacca cagctcggcg aagggtcagg
     1261 agcagcgcat gcgcgccgag gccgacgccc gcgcgcaggt gctacgcaag ttcggctgcc
     1321 gcgacatcct cgagatcggc tgcggccccg gccacttcct cgacgcggtg cgtgacctcg
     1381 gcatgcagat cgaaggcgtc gacagagccc cctcgacgga ggccgcccgc gagcgcggcc
     1441 acctcgtgca cgaggtctgg ctgcaagacc tggggcccgt cacaccacgc ttcgatgcgg
     1501 tcgccatgtg ggaggtcatc gagcacgtcg gtgaccccgc ggccgtgttg cagcgcgtgc
     1561 gccagtggct gcggccgggc ggctttttgg cgctgtcgac cccgagcagc agcgggatcc
     1621 ctgcacgcgt gctcggctcg cggttcccga tggtgtgccc gcccgagcac ctgacgctgt
     1681 tcagccgcaa gggcctcgcc gccctgctcg gcgcagccca cttcgatcct gtccgctgga
     1741 ccagcttctc cggcctcggg cgcgaacagc tgcgtcgcgg cttccagcgc ttcctgctcg
     1801 gcggcagctt gcccggacgc gcggtcgcca gcggcctcgc ggcggtcgcc gaggtgccgg
     1861 tacaactgat ggaccgggcc gggctcggca gcggcttcga ggtctactcg gtcgccacgc
     1921 ccgactgacc ggcgatgtca ggcgccggtg acgttcggcg cgacgtgggt gacctcgccg
     1981 tcgagcagct cgctgaccca gtcgaccagg cgcacgccgt cgacctcggt gttgtagaac
     2041 gcccagtgcc cgatccacgc gtggtcgacg ccggggatga tgtacgtgct ccaattgccg
     2101 gtcggcttga gcatcgcgtc gacgaagtcg tggatgccct cggcgaagcg attgctggga
     2161 tagatcgcgc cgaggccggc gcactcgttg aggccgaagc tgaagaacgt cgagatcgtc
     2221 cggtcgcgct cgctcgagat caggcccata cgctgctgcg agtgccgggt cccgatccac
     2281 cgcacgagct cggagatcga tccaccatcc tccgcccagc aatctttgca agtcaggggg
     2341 atcgtatcgt cgaggcccca cgcgtcgcgc cacatttgct gtaagcacgg cgcgaggtaa
     2401 ggatctcgca tcgccgggcc ggagtcgtcg agcagcgtca ccttgccgga gaagatgcgg
     2461 gcgatgcgat gataattgaa ggccgccccg aagccgcccg cgctgacccc cgtgaccagc
     2521 acgtgcgagg aatcgccgaa gctcggcacg atacgtttta aaaattgctc gacgttggtg
     2581 tagccgtgaa attgctggac gccctgcacg tcggcgatct cgatgtcgct gttggacccc
     2641 gcgtggacgt cccccgtgca atacggcacg tacacgaacg accagtcgcg caccgggttg
     2701 tcctcgtgcg agctgtcgaa gatgccgccg aagccgggcc cgtactgcca gtcgttgaag
     2761 tccggcagcc cgaagctgcc gaggttcgcc aggcaggtga acccattgaa gcaggcgccg
     2821 ccgccctcga agaagatcac cagcttgtcg gtcgagccgc gccgcacgcc gatgcccgtg
     2881 ctgctgccgt cgcggcagcg gctgtccggg aagtccaccc attgccacaa accaacgggc
     2941 gcgtccggga tcggctcccc gtcgaactcc ggcagcggca gctccgggtc ctccggctcc
     3001 gcgagcgccg ggccctcgcc cgacgacg
//
LOCUS       DASWNJ010000784         2223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_155856, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000784 DASWNJ010000000
VERSION     DASWNJ010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155856"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1355)
                     /locus_tag="VGB85_13115"
     CDS             complement(<1..1355)
                     /locus_tag="VGB85_13115"
                     /inference="COORDINATES: protein motif:HMM:NF015211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HEY0135025.1"
                     /translation="MQTPDQGRMLKAAKRLLKARKAGKHLIDFTEFTMPSLVDPEDSD
                     LSRYDAQYFHRALGATLEEVAEGRIKRLIITFPPRHGKSELTSKRFLAWLAGRFPDKS
                     WIFGTYNQDFADDFGRSVRGIMQHPAYGQVFPGVELRKGSAAADRLGIENGGDLVFVG
                     RGGSVTGRGGHGIVIDDPLKNSEEADSAGTREGLWAWLLNDIFSRLMDDTGFIILIMT
                     RWHEDDPVGRLTDPTNPCYNAEEAAQWQVFNIPAIAEDNDVLLRSPGEPLWPSRFGLD
                     YLLAFKRRNPRGFNSLYQQRPTPEDGELFKAEHIQTYLPHERPPLGELTIYGMSDHAV
                     ALKQENDRTCLLIVGVDQHENIYLLDCWWKRAPTDVVVEAMIDLIERWGPCKWWADND
                     HIKKSIGPFLKKRMRERKIYCPIYEQTAYADKVKKAQPIIARMSMGMIYFPAQAHWFQ
                     DA"
     gene            complement(1357..1779)
                     /locus_tag="VGB85_13120"
     CDS             complement(1357..1779)
                     /locus_tag="VGB85_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135026.1"
                     /translation="MSSVPHMPAEPTMADVANHLRALHDCVHDGQQEQTRRAEEVRLE
                     LREHRHESRNNAQKVQIQFGQLGERIAKNEGKLEIIGQTVGAPKSAATAGAVGLKFMN
                     LKWWQAVLALGGALAGATGGYKIIASAAKAFHVAMIGG"
     gene            1822..2166
                     /locus_tag="VGB85_13125"
     CDS             1822..2166
                     /locus_tag="VGB85_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135027.1"
                     /translation="MLNTFVRFVQQAKRWQVPSMLTPIIVLTASWAFLLWSVWGPGHS
                     RHKAGIAEIERDLAVLDTEPEHHREAASLRSLIDTMNKTQGTLRLFSLILPLLILLLI
                     AVEVMRATGRIG"
BASE COUNT          352 a          722 c          709 g          440 t
ORIGIN      
        1 gcgtcttgga accaatgggc ctgagccggg aagtagatca tgcccatgct catccgagct
       61 atgatcggct gggccttctt gaccttgtcg gcgtaggcgg tctgctcgta gatcgggcag
      121 taaatcttac gctcgcgcat ccgcttcttg aggaacgggc cgattgattt cttgatgtgg
      181 tcgttgtccg cccaccactt gcacgggccc cagcgttcga tgaggtcgat catggcctca
      241 accaccacgt cggtcggcgc gcgcttccac cagcagtcga gcaggtagat gttctcgtgc
      301 tgatcgacgc cgacgatgag cagacaggtc cggtcgttct cctgcttcaa cgcgacagcg
      361 tgatcgctca tcccgtagat cgtcagctcg cccagcggcg ggcgctcgtg cggcaggtag
      421 gtctggatat gctccgcctt gaacagctcc ccgtcctcgg gggtcgggcg ctgctgatag
      481 aggctgttga acccgcgcgg attgcggcgc ttgaaggcga gcaggtagtc caggccgaac
      541 cgtgacggcc acagcggctc gcccggtgag cgcaggagga cgtcgttgtc ctcggcgatg
      601 gccgggatat tgaacacctg ccactgagcg gcttcctcgg cgttgtagca ggggttggtc
      661 ggatcggtca gacggccgac cggatcgtcc tcgtgccagc gcgtcataat caggatgatg
      721 aagccggtat catccatgag gcggctgaaa atgtcgttga ggagccacgc ccacaggccc
      781 tcgcgcgtcc ctgcgctgtc ggcttcctcg ctgttcttga gcgggtcgtc gatgacgatg
      841 ccgtgcccgc ctcggccggt gacggaaccg ccccgcccta cgaacacgag gtcgccgccg
      901 ttctcgatcc cgaggcgatc cgcagcggcc gagcccttcc gcagctcgac gccggggaac
      961 acctgcccgt aggccgggtg ctgcatgatc ccgcgcacgc tacggccgaa gtcgtcggcg
     1021 aagtcctgat tgtacgtccc gaaaatccag ctcttgtcgg ggaagcgccc cgcgagccac
     1081 gcgaggaacc gcttggacgt cagctcggac tttccgtgcc gaggcgggaa ggtgattatc
     1141 aggcgcttga tccgcccctc ggccacctcc tccagcgtcg cgccgagcgc acggtggaag
     1201 tattgggcgt cgtagcggga gaggtcgctg tcctcggggt ccacgagcga cggcatcgtg
     1261 aactcggtaa agtcgatcag gtgctttcca gccttgcgcg ctttcagcag ccgcttggcc
     1321 gctttcagca tccggccctg atcgggggtc tgcatctcag ccgccgatca tggcgacgtg
     1381 gaacgccttg gccgcgctgg cgatgatttt gtagccgccg gtcgcaccgg ccagggcccc
     1441 gcccagcgcg aggacggcct gccaccattt caggttcata aacttgaggc ccacggcccc
     1501 ggcggtcgcg gcgcttttcg gtgcgccgac cgtctgcccg atgatttcga gcttgccctc
     1561 gttcttggcg atccgctcgc ccagctgccc aaactggatt tggaccttct gcgcgttgtt
     1621 ccggctctcg tgccgatgct ctcgcagctc cagccgcacc tcctcggcgc gtcgggtctg
     1681 ctcttgttgg ccgtcatgga cgcagtcgtg cagcgcccga aggtggttcg ctacgtcggc
     1741 catcgtcggc tcggctggca tgtggggcac gctgctcatg gcggaaggaa ccggatcggg
     1801 tgtgcaacag gactcccgcc gatgttgaac acttttgttc gatttgttca acaagcaaaa
     1861 cgctggcagg ttccgagcat gttgacaccc atcatcgtcc tcacggcctc ctgggccttc
     1921 cttctctggt ccgtttgggg gcccggccat agccgccaca aagccgggat cgccgagata
     1981 gagcgggacc tcgccgttct cgacaccgag cccgagcatc accgggaagc ggcgtcgctg
     2041 cgctcgctca tcgacaccat gaacaagacg cagggcacgc tgcgcctgtt cagcctgatc
     2101 ctgccgctgc tgatcctgct gctgatcgcg gtcgaggtca tgcgcgccac gggcaggatt
     2161 ggctgatgga cctgaaagcc ctgatccttc acgagatatt gaccgacgac agcgagacga
     2221 gct
//
LOCUS       DASWNJ010000785        20959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_507655, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000785 DASWNJ010000000
VERSION     DASWNJ010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 20959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20959
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507655"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1629)
                     /locus_tag="VGB85_13130"
     CDS             complement(<1..1629)
                     /locus_tag="VGB85_13130"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY0135028.1"
                     /translation="MRPDAWHIWIDRGGTFTDCVGRDPATGRLRAVKLLSSDRAPLVG
                     IRRLMGLSDDAPVPPCHVRMGTTIATNALLERRGAPCALVITRGFADLLAIGDQTRPD
                     LFALAIAPRELLHSRVLEVDARQDAEGRVLLRPEADAVDSALKQLRADGVRSVAVALL
                     HAHRDGALEQEVGEAARSAGFEHVTLSHEVAGAQGLLARAETAVVDAYLTPLLRDYVA
                     LLLSELPGSDLQIMQSSGDLTPARRFRGRDAVLSGPAGGVVAIAAVAQALGLQQVIGF
                     DMGGTSTDVTRFAGSFERRHEAEVAGVRVRTPMLAIHTVAAGGGSVCRVDGGRLTVGP
                     DSAGADPGPLCYGRAKAELLTITDVNLVLGRLGGARFPFALHAEPAHAALAEMAEQLA
                     ATGIERTPMQVAEGLRRVADEHMAAAIREVSLRRGHDVRDHAMIVFGGAGGQHACAVA
                     RLLGVRTLVFHPLAGVLSAYGMGAARVGWHGEADAGQVALSDAAIAALEDRFVALELR
                     GRATLADTAELVALRSVDLRYQGTHTAQTLALADA"
     gene            1700..3514
                     /locus_tag="VGB85_13135"
     CDS             1700..3514
                     /locus_tag="VGB85_13135"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEY0135029.1"
                     /translation="MDHEPKFLRHLRRRANQVFARWLPQPARFVYGPQYQFAWPGMPI
                     DEVRGERVLTALAAEGLLRREHIRQPPLASYHAILRVHADAYIESAQTAATMSQIVGM
                     PVSDRDTQRILDLQRSMTGGTMLATDIALSTGDLAINLGGGFHHARPALGRGFCVFND
                     VAVAIAEQRALGFHGKVLVIDLDMHDGDGTRAIFAADTSVHTLSIHSRDWDTAPALES
                     TRVALGDDVDDATYLDAVRTHVSVALRQFRPKLVYFLAGVDGAADDPLGDWSLTDETL
                     QARDEADFELLRHLAPDAAVVVTLAGGYGPGAWRHYARSFAWLQSGHHGFRPPATDEL
                     TLMRARHLAKLLREEELTGRSADDLGLTLEDVMPGHGSQESRLLGYYSRQGLELALER
                     HGFLDRLRGKGFKAPTLVCDFDDPSRQTITIFSDAVRSERLVELVLHRERRVVPGFEL
                     LSVDWMLLQNPRESFGEARPRLPGQQLPGLGLFEELVALLVVACERVGLAGLLVTPSQ
                     FHLALQWQRRLRFISPRDGARLRALREALGGISLSVMTTTLQARRVIDRRTGEPVEYQ
                     PAPMVLPLSPELQAALDSEGYTQAMDAVADEFQFELRP"
     gene            3708..5120
                     /locus_tag="VGB85_13140"
     CDS             3708..5120
                     /locus_tag="VGB85_13140"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEY0135030.1"
                     /translation="MKTDMKKLGLVLSMVVTAGLGLTACDTPDEAIDDEVSLRSDAPV
                     GLPQHVRGLITANKITPIAAPPTVSPAMFALGEALFYDKILSGTRDVACATCHAPQFA
                     SADARTLNAGINGSGVGPARTGLLGGRHTQPLFNLHLLPGNLTIDSKVELINGLVSPL
                     GLGTSFIPYPVQQLLGFDVVNTQAILPIATGQEMLGFPDPADHNEVGDCAPFDLGCVW
                     GAYMVRIKAIPQYQTLFTAAFPNRPWAQIGITEVGVAIGSYEYAAFSFDDSPWDRFVA
                     GDNAALSPAALRGAVTFFTPGKGNCVACHSGNAFTDLKFHKTLTPQFGPGGTVPGGDG
                     PGGLDDFGRERNSHNRADRYAWRTPPLRGVELTAPYGRLGQYTELEDHILHYINPTQS
                     LLNYDITQLDQTVLHGTLLNNTQAILAEGVDPLLSTVHITPADVPDLVAFLYSLTDDA
                     ERDLNHLIPATVPSGLPVDQ"
     gene            complement(5192..6976)
                     /locus_tag="VGB85_13145"
     CDS             complement(5192..6976)
                     /locus_tag="VGB85_13145"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HEY0135031.1"
                     /translation="MTTPGTLTAALAAVAHREHNGYSHLVHEKEPARFVSYAEMWGRA
                     QRCGAALQAMGVQRGDRVGLILPDSSEFIDCIFGAMLAGAIAVPIYPPMNLGQFDAYL
                     ANTTHILRQAGCSVVITDGKVRPILGKLMSDVPGLRVIETYAAALAGVAQAAEPAGVL
                     VKPDDVAFLQFTSGSTSRPKGVTLTHANLMANIAAIGGGAGIQISRESSGVSWLPLYH
                     DMGLIGFVFTPIVFDTKGILFISPLMFLKRPSVWLRQLSEQRASITFAPNFAYGLCTT
                     RVKDSELAGVDLSQLQVAGCGAEPIQYGTLRAFADRYAPYGFRSTAFLPCYGMAEHSL
                     AVTFVDLHADLKSDRVDAGALAGRSAVPARDGAPAVDVVCCGRAFPGHALQIVDDTGA
                     PVGDRVVGEIELRGPSVMQGYWNDPERTAEAFHDGWLRTGDLGYLVDGELHVCGRSKD
                     LIILHGRNYHPQDIEWQASQVEGVRRGNVIAFGLHHPDLGREYVVVAAEVRQVGEGDV
                     DDPMRRQIETRVLEALALKVDEVVLLPPGSLPKTSSGKLQRRKAAEMFGNGELGKGVG
                     PASKLGLLKHLAASRWGFIKASLGGREG"
     gene            complement(6973..7263)
                     /locus_tag="VGB85_13150"
     CDS             complement(6973..7263)
                     /locus_tag="VGB85_13150"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY0135032.1"
                     /translation="MSNNGEHSYDNVLAKIFEYLEEHASERPSTPVSASSRLIQDLGL
                     DSLQSFEMVSDLEDSFNITIPMDLFQGVETLEDVAHAVLRVIAGERSVGARA"
     gene            complement(7413..8561)
                     /locus_tag="VGB85_13155"
     CDS             complement(7413..8561)
                     /locus_tag="VGB85_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135033.1"
                     /translation="MTSRAVELIEVPLSDRKLVERFIRVPWYVNREHHPSEQWVPPLL
                     MDRRDYLNPAKNPFFEHVQAAFWIARVDGRDVGRVAAVEDADYIKFHGEQTGYLGMFE
                     CPDDHTVAQALVDKAKGWLRARGMTSMIGPFELSTNYISGVLVEGFEREPGINMPYNP
                     PYYDRLLTACGMQKAKDLLHWELDPQKPTPERIVRVAEKMAARSGVKLRKMSFDDWDA
                     EVLRTLEVYNDAWEKNWGFVPIGKKEYLHIAKDLKMVLHPDFALLAEVDGKPAAFVLT
                     IQNVNPVMKKLDGKLFPFGIFRLLWDLKVKKTIKAGRLILLGIKAQYRGQGIDSLLFL
                     ATHRAGAQQGWTSGEIGWTLEDNFKVNSAIKSMGGYQVATYRVYETAL"
     gene            8685..9650
                     /locus_tag="VGB85_13160"
     CDS             8685..9650
                     /locus_tag="VGB85_13160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibro-slime domain-containing protein"
                     /protein_id="HEY0135034.1"
                     /translation="MRTAPSIARARWLLATTSLLACGACGACGDSGKAADTGSSSPTQ
                     ITTVSEPGPTDTGDATQSATTSTSGQVPTEAGSTAGSGTGETVGSTSTTTGGTTGPGD
                     PSASETAFDTGFDCVQTLQATVRDFRAEHPDFEDFTSETGLKGIVEETLGPDMKPVYA
                     TDGATAQTSGPGNYAQWYNNTPDTNIAFAVDLPLTEVMPGLFSFESDAFFPLDAQGWG
                     NQGNPHNFHFTTEIHTTFNYQGGEVFTFIGDDDLWLFINGELAIDLGGVHPELTGTVD
                     LDAEAAALGIAIGGTYAMDIFHAERHTVDSNFRIDTSIQCFIIPG"
     gene            9775..10866
                     /locus_tag="VGB85_13165"
     CDS             9775..10866
                     /locus_tag="VGB85_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibro-slime domain-containing protein"
                     /protein_id="HEY0135035.1"
                     /translation="MVALALASACGGSSGTSASGSDTDASSSSTGTSNNSFPTTGDMT
                     AGSESNSGSTTDTPTSGNSESNSNSGVTDTSTSTSSVSASESGSDSSGATATETTDPN
                     TSTTTGDTTGGESTMGVSDSDSASTGGTTGGGESTTGADDTTTGTDDTTTTGGDADCD
                     PVLDVMMRDFKVEHPDFEKYGGNVAYKGLVKEDLGPDKKPVYAAAGPTPQTSGPEAFK
                     QWYNDTPNINQAIPIQLTLTEIMPGIFQYKSDAFFPLDNQGFGNQGFGHNFAFTTEIH
                     TQFIYNGGESFTFTGDDDMWMFINNKLAIDLGGLHPPDTQTINLDAEAAKLGITKGLV
                     YSMDIFHAERHSTGSNFRIDTTIGCFIPQ"
     gene            complement(10853..12991)
                     /locus_tag="VGB85_13170"
     CDS             complement(10853..12991)
                     /locus_tag="VGB85_13170"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY0135036.1"
                     /translation="MATAVPASVLADPVLAGFRGEIRPVPSTWLYRVAIVLVAVLMLL
                     LPLVYLAIIGALVWVMVWHATHNAGMIDGAGQKGLLVAFAYVAPFVIGGLGVFFLLKP
                     LLARRAPDPASRVLDPAEEPLLFAFVEKICNAVHAPRPARIVVTCAVNASASLRRGVL
                     SFFGNDLVLTIGLPLVACQTLPQFTGVLAHEFGHLSQSLGMRAVYVIRRVNHWFARVV
                     YERDAWDLRVERWIAASRKIHLFLSGLGYAVQGMIWLSRRVSWALMWVGHVMSSVLLR
                     EMELDADRHALRLVGAEAFGSALREVAVISVANDGAMHDLGAAWRSRRLPDDLPALVR
                     ANIAQLEPEVQQKIVEHGLTENTTMYASHPAIRDRLAAIREQDPPMFTGEGAATRLFR
                     DFAALAREVTLAEYRQLLADEIGPTNLVSTAQLVGEVERKMTGARAAQRVYGELVGWL
                     ILDVGALTGIVGVQGGSLRDQLRAAREALAAEMPAVAKAVPELVQCETLLTEMITLRL
                     YREAQVKVDFASFGLAADTQAAFDEVYARRLQQRSLLLATCRPAVEATLQRISAAGYA
                     LGEPADQAEYARLTDCLRTLLVVRPLVEAVRAHLVAYTNIDAKVREHAGLMPALSRAL
                     AHQHARLQQLRAGLAEHAYPYPHARGAATLADYALPELPPVDALAAILDASGACMAQL
                     DNLGQRVLAELASLTERAEEELNPVELTAG"
     gene            complement(13003..13518)
                     /locus_tag="VGB85_13175"
     CDS             complement(13003..13518)
                     /locus_tag="VGB85_13175"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEY0135037.1"
                     /translation="MLEGFRGKFPRVDPTAYVHPSATLIGDVEVGPGASIWPGAVLRA
                     DDGPIVIGANTSIQDGTVIHMTEGRSRTTVGARVTVGHRVILHGCTIGDDCLIGMGSC
                     LLDNAVVEDWAFVAAGTLVPPGKVVPTGQLMMGNPGKLVRPMTDADREWITYSWTAYF
                     NRSREYMAQTP"
     gene            complement(13520..15016)
                     /locus_tag="VGB85_13180"
     CDS             complement(13520..15016)
                     /locus_tag="VGB85_13180"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0135038.1"
                     /translation="MTSQRYRPLYKLDAGGMAEVYVAEAESMAGFKKKVAIKRILPGL
                     IKDQRFVRMFLDEARLSLRLNHANVVSVFDIGESENTYFIVMDFVHGTNLKALLEYQA
                     KRGGAMPVPLVTWILTEILKGLAYAHKLTDSETGRPIGIVHRDISPPNILVSWNGEVK
                     LTDFGLAKATTQLESTDPGVVKGKYSYLSPEAARGEEIDHRADVFSAGILAFEMLTGR
                     RLFRGKNDYQTIALVRACEVPSVRSYNPAVPPELEAVIMRALSKDINDRYADADDFAD
                     ALLQFLFSNRLKVGARDLIEYCKPIREEIEADKRAQEKKAQEERSPGGNNLIINLINE
                     EMLHFRSLGEGEKPAQEGLASEPVVPVAEATRGSSSGSSNPNASTSGRQDVDPSRPLD
                     VDVFTPVPGKVPLTPNKLGLSPTPVEPMPRRDMSIDEELGLGGSSAKPAPGGPATGKQ
                     QPQRTIPPDPRTARPPAPTSKEGGSSWIIVLVLLLVAGGVIAFLVFGM"
     gene            complement(15093..16301)
                     /locus_tag="VGB85_13185"
     CDS             complement(15093..16301)
                     /locus_tag="VGB85_13185"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEY0135039.1"
                     /translation="MTPWILAAKLLASAAGTYFVDGALAPATGDDASAPATSSSTPGS
                     TPAIAPKKAPRAAPAAATEEPRTPAPRGEPRAAATPEIEIGIWPDDASRLPRLEPPGV
                     VRGPAPGRSPLLGWSGALAEPEFVDRTHRVPWVPRPADFGATMRPGERFKFDVYFGGN
                     PTGIAEAAVVAREPGPNGTPDRIRLEGSARTSGVAALLTTIVYDMAAILDAETGAPVM
                     TGAITKREGMATTYKRRETVATFFGRGFVEIADDKDGKTSSARKRLPADTFDPLSVMA
                     WVRSLDLEPGAKAKAHALDGNTLLRVDITARGPTRTNDLPPLAAALGITQDQVVAYDG
                     VITRVDRFGAAIPGKKAYKMRVWVSSEGRRIPLMIESDLWLGVVRLILTQYDPPHDAA
                     GSRTPAPRAG"
     gene            16433..17365
                     /locus_tag="VGB85_13190"
     CDS             16433..17365
                     /locus_tag="VGB85_13190"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY0135040.1"
                     /translation="MVTQGDPAGIGPELLLRLGAADLLRKGDVVVADPAPLRRLADSL
                     GEWAVRGLTSLEPLLDPLPHPLTSPFGQVSALIRGVDLVLADPARRALVTAPIDKAGC
                     RAEGFEFPGHTEYLAARAGVEDFAMLMAGPRLRVVLATIHLALREVPERLSIAGIVRA
                     GRLLARAMPRWVRGRPAIVGVLGLNPHAGERGMLGDEERTIVGPAVELLRQWASEHAV
                     PVEFRGPLPADTAFPAHAEGRLDGVVAMYHDQGLGPFKLLHMHDGVNMTLGLPFVRTS
                     PDHGTARDIAGRGVADPSSMFAAVALARGGEPVP"
     gene            17362..20544
                     /gene="uvrA"
                     /locus_tag="VGB85_13195"
     CDS             17362..20544
                     /gene="uvrA"
                     /locus_tag="VGB85_13195"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY0135041.1"
                     /translation="MSKVAKTSKAATKTASSKLAPAPAKPTAVKTAAVVKTTKAPRST
                     AKAVPEAAEVVALPPEIAVAETPAPASTAPASTTPANVTPTSTPAPIAAASLTPEPGT
                     PEPPPVPALAPLAPQVPAPCIRIRGARTHNLQSIDLDLPRGKLVVITGPSGSGKSSLA
                     FHTIFAEGQRRYVESLSASARQFLAQLPKPDADLIEGLSPAIAIEQSPGGRNPRSTVG
                     TSTEVYDFLRLLYARVGEVYSYKTGKPIRRHTIEDMLAGTLALPPGTRFSVIAPVVRG
                     QPGDHAELLADLRRQGFSRVAIDDEVRDLADTITLDPGKRHTIEVYVDRLVMKEGVRG
                     RLADSFEVALRLTGGLVKILTIEGQELLFSEQYADFDHGLAYPEITPSLFSFNSPDGA
                     CPECDGLGSRRVFDPRRLVPNEHLSLKEGALHVWTRRGGRASTQALQALSEHYKFDLF
                     APWIKLPDEVKIVLLQGSGDEAIPALGKKPTPFEGVIPSLERRLREAETTAGESDEEG
                     GTLEEVEALMSEVVCTSCEGQRLRQEARMVKIGGHNISEISAWPIEQLLPFLERLELG
                     EERNEVAEAILGQSRQRLRFMLEVGLGYLTLDRTTMTLSGGEAQRIRLATQVGAALVG
                     VTYILDEPSIGLHQRDNDRLIAALIRLRDLGNTVLVVEHDEDTIRAADFLVDMGPGAG
                     VQGGRVVAAGPLQEVLANRRSLTAAYLTGRMSIPLPEKYRRSIGPALVISHATGHNLK
                     DVTVKIPLGTFTCVTGVSGSGKSSLIIDTLLPEAVRRLNGGSGFGLAHHGITGLENLD
                     KVIHVDQSPIGRSPRSNPATYTGLFAELRTLFANLSESKVRGYTPARFSFNVKGGRCE
                     SCQGDGVRRIEMHFLADVFVTCRACNGRRYNRETLAVTYRGKSIADVLAMSVSDACDF
                     FANYPALRQKLETLRDVGLGYVTLGQSALTLSGGEAQRIKLAKELAKKSTGKTLFILD
                     EPTTGLHFGDIKLLLKVLDRLVDEGNTVVVIEHNLDVIKCADHIIDIGPEGGSRGGEL
                     VASGTPPQVARVAASATGKYLARVLQAPGA"
     gene            20656..>20959
                     /locus_tag="VGB85_13200"
     CDS             20656..>20959
                     /locus_tag="VGB85_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135042.1"
                     /translation="MRLPYLLLTLAALTAAGRAAATSCGDLPWANEYSSPLENIGVAV
                     PFDAHPWRIDTCFSGPPRTPVDCSFIDDETKAAIAAEVTVTDTAACSLDLPNDAPAS"
BASE COUNT         3248 a         7835 c         6823 g         3053 t
ORIGIN      
        1 cgcgtccgcg agcgcgagcg tctgggccgt gtgcgtgccc tggtagcgca ggtcgacgct
       61 gcgcagggcc acgagctcgg cggtgtcggc cagcgtcgcg cggccgcgga gctcgagcgc
      121 gacgaagcgg tcctcgagcg ctgcgatcgc cgcgtcgctc aatgcgacct ggcccgcgtc
      181 ggcctcgccg tgccagccga cgcgcgcggc tcccatgccg taggccgaga gcaccccggc
      241 gagcgggtgg aacacgagcg tgcgcacgcc gagcagccgc gcgacggcgc aggcgtgctg
      301 gcccccggcc ccgccgaaca cgatcatcgc gtggtcgcgg acatcgtgcc cgcgacgcag
      361 cgagacctcg cggatcgcag cggccatgtg ctcgtcggcg acgcggcgca ggccctcggc
      421 gacctgcatc ggcgtgcgct cgatgcccgt cgcggccagc tgctcggcca tttcggcgag
      481 cgcggcgtgt gcgggctccg catgcaatgc aaacgggaag cgggccccgc cgaggcggcc
      541 gagcaccagg ttgacgtcgg tgatcgtcag cagctccgcc ttcgcgcgcc cgtagcacag
      601 cgggcctgga tcggcgccag cgctgtcggg tccgaccgtg aggcgcccgc cgtcgacgcg
      661 acacaccgag ccacccccgg ccgcgacggt gtggatcgcc agcatcggcg tccgcacgcg
      721 caccccggcg acctcggcct cgtgacggcg ctcgaagctg ccggcgaagc gcgtcacatc
      781 ggtcgacgtc ccgcccatgt cgaagccgat cacctgctgc aggccgagcg cctgggccac
      841 cgcggcgatc gccaccacac cgcccgcggg ccccgagagc accgcgtcgc ggccacgaaa
      901 cctgcgcgcg ggcgtcaggt cgccgctcga ctgcatgatc tggaggtcgc tgcccggcag
      961 ctcggacagc agcagcgcca cgtagtcgcg cagcagcggc gtcaggtagg cgtcgaccac
     1021 cgccgtctcg gcgcgcgcga gcaggccctg cgccccggcg acctcgtgcg acagcgtcac
     1081 gtgctcgaag cctgcgctgc gcgccgcctc gccgacctcc tgctcgagcg cgccgtcgcg
     1141 gtgtgcgtgc aacagcgcga ccgcgacgct gcgcaccccg tccgcgcgca gctgtttcaa
     1201 cgcgctgtcg accgcgtccg cttcgggccg cagcaacacc cgcccctcgg cgtcctggcg
     1261 ggcgtcgacc tcgagcaccc gcgaatgcag cagctcacgc ggcgcgatcg ccagggcgaa
     1321 caggtcgggt cgggtctgat ctccgatcgc cagcaggtcg gcgaagccgc gggtgatgac
     1381 gagcgcgcac ggggccccgc gccgctccag caacgcgttg gtggcgatcg tcgtgcccat
     1441 gcgcacgtgg cacggtggca ccggcgcgtc gtcggagagg cccatcaggc ggcgaatgcc
     1501 gaccagcggc gcgcggtcgg aggacagcag cttgaccgcc cgcaggcggc cggtggcggg
     1561 gtcgcggccg acgcagtcgg taaaagtccc gccgcggtcg atccagatgt gccaggcgtc
     1621 ggggcgcacg ggccgatcct agcacttccg tgcggggtct tggccgcggc gccgggacat
     1681 gcaaccactg aggctcgcga tggaccacga gcccaagttc ctccgccacc tgcgccggcg
     1741 cgccaaccag gtgttcgcgc gctggctgcc gcagccggcg cgcttcgtat acgggccgca
     1801 gtatcagttc gcgtggccgg gcatgccgat cgacgaggtc cgcggcgagc gcgtgctgac
     1861 ggcgctggcg gccgagggtc tgctgcgccg cgagcacatc cgtcagccgc cgctggcctc
     1921 gtaccacgcg atcttgcggg tccacgccga cgcgtacatc gagtcggcgc agaccgccgc
     1981 gacgatgtcg cagatcgtcg gcatgccggt cagcgaccgc gacacccagc ggatcctcga
     2041 ccttcagcgc agcatgaccg gcggcacgat gctcgccacc gacatcgcgc tgtcgaccgg
     2101 cgacctggcg atcaacctcg gcggcggctt tcaccacgcg cgtccggccc tcggccgcgg
     2161 cttctgtgtc ttcaacgacg tcgcggtggc gatcgccgag cagcgcgcgc tcggctttca
     2221 cggcaaggtc ctcgtcatcg acctcgacat gcacgacggc gacggcaccc gggcgatctt
     2281 cgccgccgac accagcgtgc acaccctctc gatccacagc cgcgactggg acaccgcgcc
     2341 cgcgctcgag tcgacccgcg tcgcgctcgg cgacgatgtc gacgacgcca cctacctcga
     2401 cgccgtgcgc acccacgtca gcgtggcgct gcggcagttt cgtccgaagc tcgtgtactt
     2461 cctcgccggc gtcgacgggg ccgccgacga cccgctcggc gactggtcgc tgaccgacga
     2521 gaccctgcag gcccgcgatg aggcggactt cgagctgctg cgccacctcg cgcccgacgc
     2581 cgccgtcgtc gtcacgctcg ccggcggcta cggccccggc gcctggcgtc actacgcgcg
     2641 cagcttcgcg tggctgcaga gcggccacca cggctttcgc ccgcccgcca ccgacgagct
     2701 gacgctgatg cgcgcgcggc acctcgccaa gctgctgcgc gaggaggagc tgacgggccg
     2761 cagcgccgac gacctcggcc tgacgctcga ggacgtcatg cccggccacg gctcgcagga
     2821 gtcgcggctg ctcggttatt actcgcgcca gggcctcgag ctggcgctcg agcgtcatgg
     2881 tttcctcgat cgcctgcgcg gcaagggctt caaggcgccg accctcgtct gtgacttcga
     2941 cgaccccagc cgccagacga tcaccatctt cagcgacgca gtgcgcagcg agcggctggt
     3001 cgagctcgtg ttgcaccgcg agcgtcgcgt cgtgcccggc ttcgagctgc tgtcggtcga
     3061 ctggatgctt ttgcaaaatc cgcgcgagag cttcggtgag gcgcgcccac gcctgcccgg
     3121 ccagcagctg ccgggcctcg gcctgttcga ggagctggtc gccctgctcg tggtcgcctg
     3181 cgagcgcgtc ggcctggccg gcctgctcgt caccccgtcg cagttccacc tcgcgctgca
     3241 gtggcagcga cgactccgct tcatctcacc ccgcgacggc gcccgcctgc gagcgctgcg
     3301 agaggcgctc ggcggcatat cgctgtcggt gatgacgacc acattacagg cgaggcgcgt
     3361 gatcgaccgt cgcaccggcg agcccgtcga ataccagccg gccccgatgg tcctgccgct
     3421 gtcgccggag ctgcaggcag cgctcgacag cgagggttat acgcaggcga tggacgccgt
     3481 cgccgacgag ttccagttcg agctgcgacc atgaacttgc cgcgaccgtc cgcggacagc
     3541 cgcgcacggc cgtgcagacg ctgtccgtct gccgcaaacg ttgccgctcc cgtggtcggg
     3601 cgcaccgggg cgcacatgaa ctcgcacctt gaccgagatc gctgtgatcc attaaggtgg
     3661 cgccggttgc tattggttgg gtttgttggc tgacaacagg agtgggtatg aagacggata
     3721 tgaagaagct cgggttggtt ctttccatgg tcgtgacggc agggctcggc ttaaccgcct
     3781 gtgatacgcc cgacgaggcg atcgacgacg aggtctcgct gcgctcggac gcccccgttg
     3841 gcctgccgca gcacgtccgc ggcctgatca cggcgaacaa gatcaccccg atcgcggcgc
     3901 cgccgaccgt atccccggcg atgttcgccc tcggcgaggc cctcttctac gacaagatcc
     3961 tcagcggcac ccgcgacgtc gcctgcgcga cctgtcatgc cccgcagttc gccagcgcgg
     4021 acgcccgcac gctcaacgcc ggcatcaacg gctccggcgt cggcccggcg cgcaccggcc
     4081 tgctcggcgg ccgccacacc cagcccctct tcaacctgca cctgctgccc ggcaacctga
     4141 cgatcgacag caaggtcgag ctgatcaacg gcctcgtcag cccgctcggc ctcggcacca
     4201 gcttcatccc gtatccggtc cagcagctgc tcggcttcga cgtcgtcaac acccaggcga
     4261 tcctcccgat cgccaccggc caggagatgc tcggcttccc cgaccccgcc gaccacaacg
     4321 aggtcggcga ctgcgcgccc ttcgacctcg gctgcgtgtg gggtgcctac atggtccgca
     4381 tcaaggcgat cccgcagtac cagaccctgt tcaccgccgc cttcccgaac aggccctggg
     4441 cccagatcgg catcaccgag gtcggcgtcg ccatcggctc ctacgagtac gccgccttct
     4501 cgttcgacga cagcccgtgg gaccgcttcg tcgccggtga caacgcggcc ctcagcccgg
     4561 ccgcgctgcg cggcgccgtc accttcttca cgcccggcaa gggcaactgc gtcgcctgtc
     4621 acagcggcaa cgccttcacc gacctcaagt tccacaagac cctgaccccg cagttcgggc
     4681 cgggcggcac ggtccccggc ggcgacggcc ccggcggcct cgacgacttc ggtcgcgagc
     4741 gcaacagcca caaccgtgcc gaccggtacg cctggcgcac cccgccgctt cgcggcgtcg
     4801 agctgaccgc gccgtacggc cgcctcggcc agtacaccga gctcgaggac cacatcctcc
     4861 actacatcaa cccgacccag tcgctgctca actacgacat cacgcagctc gatcagacgg
     4921 tcctgcacgg caccctgctc aacaacacgc aggcgatcct cgccgagggc gtcgacccgc
     4981 tgctgagcac ggtccacatc accccggcgg acgtgcccga cctcgtcgcg ttcctgtact
     5041 cgctcaccga cgacgccgag cgcgacctca accacctgat ccccgcgacg gtccccagcg
     5101 gtctgccggt cgatcaatag gccgcatccc ggcaaccggg tctccagctg tcacgcccga
     5161 cgggtcctcg acccgccggg cgtcggcaat ttcacccctc gcggccgccg agcgacgctt
     5221 tgataaatcc ccagcgcgac gccgccaggt gcttgagcag gccgagcttg ctcgccggtc
     5281 cgacgccctt gccgagctcg ccattgccaa acatctccgc cgccttgcgg cgctgcagct
     5341 tgccgctcga ggtcttgggc aggctgccgg gcggcagcag cacgacctcg tcgaccttga
     5401 gcgccagcgc ctcgagcaca cgcgtctcga tctgccggcg catcgggtcg tcgacgtcgc
     5461 cctcgccgac ctgacgcacc tcggccgcga ccaccacgta ctcgcgcccg aggtccgggt
     5521 gatgcagccc gaacgcgatc acgttgccgc gacgcacgcc ctcgacctgc gaggcctgcc
     5581 actcgatgtc ctgcgggtgg tagttgcggc cgtgcaggat gatgaggtcc ttgctgcggc
     5641 cgcacacgtg cagctcgccg tcgaccaggt agccgaggtc gccggtgcgc agccagccgt
     5701 cgtgaaacgc ctcggccgtg cgctcggggt cgttccagta gccctgcatc acgctcggcc
     5761 cgcgcagctc gatctcgccg acgacgcggt cgccgaccgg cgcgcccgtg tcgtcgacga
     5821 tctgcagcgc atggcccggg aaggcccgcc cgcagcagac gacgtcgacc gcgggcgccc
     5881 cgtcacgcgc cggcaccgcg ctgcgtcccg cgagcgcgcc ggcgtcgacg cggtcggact
     5941 tgaggtccgc gtgcaggtcg acgaaggtga ccgcgaggct gtgctcggcc atcccgtagc
     6001 acggcaagaa cgcggtgctg cgaaacccgt agggcgcgta gcggtcggcg aaggcgcgca
     6061 acgtgccgta ctggatcggc tcggcgccgc agcccgcgac ttgcagctgc gagaggtcga
     6121 cgccggccag ctcgctgtcc ttgacccgcg tcgtgcacag cccgtacgcg aagttcggcg
     6181 cgaaggtgat cgacgccctc tgctccgaca gctggcgcag ccacaccgac ggccgcttga
     6241 ggaacatcag cggcgagatg aacaggatgc ccttggtgtc gaacacgatc ggcgtgaaca
     6301 cgaagccgat caggcccatg tcgtgataca gcggcagcca gctgacgccg ctcgactcgc
     6361 gcgagatctg gatccccgcg ccgccgccga tcgccgcgat gttggccatc aggttcgcgt
     6421 gcgtcagcgt cacgcccttg ggccgcgagg tgctgccgga ggtaaattgg agaaaagcga
     6481 cgtcgtccgg cttgaccagc acaccggccg gctcggccgc ctgcgccacc ccggcgagcg
     6541 ccgccgcgta ggtctcgatc acgcgcagcc cgggcacgtc gctcatcagc ttgccgagga
     6601 tcggccgcac cttgccgtcg gtaatgacga cgctacagcc cgcctgccgc aagatatggg
     6661 tcgtgttggc gaggtacgcg tcgaactgcc cgaggttcat cggcgggtag atcggcaccg
     6721 cgatcgcgcc ggcgagcatc gccccgaaga tgcagtcgat gaactccgac gagtccggca
     6781 agatcagccc gaccctgtcc ccccgctgca cccccatcgc ctgcaacgcc gccccgcagc
     6841 gctgcgccct gccccacatc tccgcgtacg agacgaagcg cgccggctcc ttctcgtgca
     6901 cgaggtggct gtagccgttg tgctcgcggt gcgcgacggc ggcgagcgcc gcggtcaacg
     6961 tgccgggcgt cgtcatgccc gcgcccccac gctccgctcg cccgcgatca cccgcagcac
     7021 tgcatgcgcc acgtcctcga gggtctcgac gccctgaaac aggtccatcg ggatcgtgat
     7081 gttgaacgag tcctcgaggt ccgagaccat ctcgaagctc tgcaaggagt ccaacccgag
     7141 gtcctggatc agccgcgagc tggcgctcac cggcgtcgac ggccgctcgc tcgcgtgctc
     7201 ctcgagatac tcgaagatct tggccaggac gttatcatag ctgtgctcgc cgttattcga
     7261 catggtgctc cccgtctcgt cgcggcgtca taccagaact ggcgcgcgag gcaaccacgg
     7321 gtccccctga acagctccct ggcccgcgac gggagcccat catttcgctg gaatggagct
     7381 ccccgggcct ccgacgtgcc ggccggtcgc gatcagagcg ccgtctcgta cacgcgatac
     7441 gtggcgacct gatacccgcc catgctcttg atcgcgctgt tgaccttgaa gttgtcctcg
     7501 agcgtccacc cgatctcgcc cgacgtccag ccctgctgcg cccccgcgcg gtgggtcgcg
     7561 aggaacagca gcgagtcgat gccctggccg cggtactggg ccttgatgcc gagcaggatc
     7621 aggcggccgg ccttgatcgt ctttttgacc ttgaggtccc acagcaggcg aaagatgccg
     7681 aacgggaaca gcttgccgtc gagcttcttc atcaccggat tgacgttctg gatcgtcaac
     7741 acgaaggcgg ccggcttgcc gtcgacctcg gcgagcagcg cgaagtcggg gtgcaggacc
     7801 atcttgaggt ccttggcgat gtgcaggtac tccttcttgc cgatcggcac gaagccccaa
     7861 ttcttctccc acgcatcatt gtaaacctcg agcgtgcgca ggacctccgc gtcccagtcg
     7921 tcgaagctca tcttgcgcag cttgacgccg ctgcgcgccg ccatcttctc ggcgacccgg
     7981 acgatgcgct cgggcgtcgg cttttgcggg tcgagctccc agtgcagcag gtccttggcc
     8041 ttttgcatcc cgcacgccgt cagcaggcgg tcgtagtacg gcgggttgta cggcatgttg
     8101 atgccgggct cgcgctcgaa gccctccacc agcacgcccg agatgtaatt cgtcgagagc
     8161 tcgaagggcc cgatcatgct ggtcatgccg cgcgcgcgca gccagccctt cgccttgtcg
     8221 accagcgcct gcgcgaccgt gtggtcgtcc gggcactcga acatcccgag gtagccggtc
     8281 tgctcgccat ggaacttgat gtagtcggcg tcctcgaccg cggcgacgcg cccgacatca
     8341 cggccgtcga cgcgcgcgat ccaaaaggcc gcctgtacgt gctcgaagaa cgggttcttg
     8401 gccgggttga ggtagtcgcg gcggtccatc agcagcggcg gcacccactg ctcgctcggg
     8461 tgatgctcgc ggttcacgta ccagggcacg cgaatgaagc gctcgaccag cttccggtcg
     8521 ctcaggggca cctcgatcag ctcgaccgcg cggctcgtca ttcactaatc tccgggttcg
     8581 cggatagccg tgcacccgcc cgcgcgtcaa gtgcgcgccc ggcgctgttc gccgaacgtg
     8641 ccgcgagatt gacggccccg acgacgcggg ctaccgctcg agcgatgcgt actgccccct
     8701 cgatcgcccg cgcccggtgg ctcctcgcca ccacgtcgct cctcgcttgc ggtgcatgcg
     8761 gtgcatgcgg tgacagtggc aaggcggccg acacgggctc gagctcgccg acgcagatca
     8821 cgaccgtcag cgagcccggc cccaccgaca ccggcgacgc gacgcagagc gcgaccacca
     8881 gtacttcggg acaggtaccg accgaggccg gcagtaccgc gggtagcggc accggcgaga
     8941 ccgtcggcag cacctccacc acgaccggcg gcacgaccgg accgggcgac ccgagcgcga
     9001 gcgagaccgc cttcgacacc ggcttcgact gcgtgcagac cctccaggcg accgtccgcg
     9061 actttcgtgc cgagcacccg gacttcgagg acttcaccag cgagaccggt ctcaagggca
     9121 tcgtcgagga gacgctcggc cccgacatga agccggtgta cgcgaccgac ggtgcgaccg
     9181 cgcagacctc cgggccgggc aattacgcgc agtggtacaa caacacgccg gacaccaaca
     9241 tcgccttcgc ggtcgacctg ccgctgaccg aggtgatgcc tggtctgttc agcttcgaga
     9301 gcgacgcctt cttcccgctc gacgcccagg gctggggcaa ccagggcaac ccgcacaatt
     9361 tccacttcac caccgagatc cacacgacct tcaactatca gggtggtgag gtctttacct
     9421 tcatcggcga cgacgacctg tggctgttca tcaacggcga gctggcgatc gacctcggcg
     9481 gcgtgcaccc cgagctcacc ggcaccgtcg acctcgacgc ggaggctgcc gcgctcggca
     9541 tcgccatcgg cggcacctac gcgatggaca tctttcacgc cgagcgccac accgtcgact
     9601 ccaactttcg catcgacacc agcatccagt gcttcatcat ccccggatga ccgacacatg
     9661 acggacacac gtgcatgagc gccgccgcgc acccgcagat cctcggttga cggcccgtgg
     9721 tttcgcgtcg acgatgcgac catgaaacgt ggattacatg atcttggccc ggccatggtc
     9781 gccctggcct tggcctcggc gtgcggcggc tcgtccggca cgagcgccag cggcagcgac
     9841 accgacgcct cgagctcgag caccggcacc agcaacaaca gcttcccgac caccggtgac
     9901 atgaccgccg gcagcgagag caacagcggc tcgaccaccg acacgccgac ctccggcaac
     9961 agcgagagca acagcaacag cggcgtcacc gacacgtcga cgtcgacctc ctcggtctcc
    10021 gccagcgagt ccggcagcga ctcgagcggc gccacggcga ccgagacgac cgacccgaac
    10081 accagcacga ccaccggcga caccaccggc ggcgagagca cgatgggcgt cagcgacagc
    10141 gacagcgcca gcaccggcgg gaccaccggc ggcggcgagt cgacgaccgg cgccgacgac
    10201 acgacgaccg gcaccgacga cacgacgacc accggtggcg acgccgactg tgaccctgtg
    10261 ctcgacgtga tgatgcgcga cttcaaggtc gagcacccgg acttcgagaa gtacggtggc
    10321 aacgtcgcgt acaagggtct cgtcaaggag gacctcggac ccgacaagaa gccggtctac
    10381 gccgccgccg gcccaacccc gcagaccagc gggccggagg cgttcaagca gtggtacaac
    10441 gacacgccga acatcaacca ggcgatcccg atccagctga cgctgacgga gatcatgcct
    10501 ggcatcttcc agtacaagag cgacgccttc ttcccgctcg acaaccaggg cttcggcaac
    10561 cagggctttg gacacaactt cgccttcacg accgagatcc acacgcagtt catctacaac
    10621 ggcggcgagt cgttcacctt caccggcgac gacgacatgt ggatgtttat taacaacaag
    10681 ctggcgatcg acctcggggg cctgcacccg cccgacaccc agacgatcaa cctcgacgcc
    10741 gaggccgcca agctcggcat caccaagggc ctcgtctact cgatggacat cttccacgcc
    10801 gagcgccact cgaccggctc gaacttccgc atcgacacca cgatcggctg cttcatcccg
    10861 cagtgagctc gaccgggttc agctcctcct ccgcgcgctc cgtcagcgac gccagctcgg
    10921 ccagcacgcg ctggccgagg ttgtcgagct gcgccatgca ggccccgctc gcgtcgagga
    10981 tcgctgccag cgcgtcgacc ggcggcaact cgggtaacgc gtagtcggcg agcgtcgcgg
    11041 ccccacgcgc gtgcgggtac gggtacgcgt gctccgccag ccccgcgcgc aactgctgca
    11101 gacgcgcgtg ctggtgcgcg agcgcccgcg acagcgccgg catcaggccg gcatgctcgc
    11161 gcaccttcgc gtcgatgttg gtatacgcga ccaggtgtgc gcgtaccgcc tcgacgagcg
    11221 ggcgcaccac gagcaacgtc cgcagacagt cggtgagccg cgcgtactcg gcctggtccg
    11281 ccggctcgcc gagagcgtag cccgcggccg agatccgctg cagcgtcgcc tcgacggccg
    11341 gacgacaggt cgcgagcagc agcgagcgct gctgcaggcg ccgggcatag acctcgtcga
    11401 aggccgcctg cgtatcggcg gcgagcccga acgacgcgaa gtcgaccttg acctgcgcct
    11461 cacgatacag ccgcagcgtg atcatctcgg tcagcagggt ctcgcactgg accagctcgg
    11521 gcaccgcctt tgcgaccgcg ggcatctcgg ccgcgagcgc ctcccgggcc gcgcgcagct
    11581 gatcgcgcaa cgaaccacct tgcactccca cgatcccggt caacgcgccg acatcgagga
    11641 tcagccagcc gacgagctcg ccgtagacgc gctgggccgc gcgtgccccg gtcatcttgc
    11701 gctcgacctc accgacgagc tgcgccgtcg agaccaggtt cgtcgggccg atctcgtcgg
    11761 cgagcaactg gcggtactcg gcgagcgtca cctcgcgggc cagcgccgcg aagtcgcgga
    11821 acaggcgggt cgctgcgccc tccccggtga acatcggcgg gtcctgctcg cgtatcgccg
    11881 cgagccgatc gcggatcgcc gggtggctgg catacatcgt cgtgttctcg gtcagcccgt
    11941 gctcgacgat cttctgctgc acctccggct cgagctgggc gatgttggca cgcacgagcg
    12001 cgggcaggtc gtccggcagc cggcggctgc gccacgcggc gccgaggtcg tgcatcgccc
    12061 cgtcgttggc gacgctgatc accgcgacct cgcgcagcgc cgaaccaaag gcctccgcgc
    12121 cgaccaggcg cagcgcgtgg cggtccgcgt cgagctccat ctcgcgcagc agcacgctgc
    12181 tcatcacgtg cccgacccac atcagcgccc acgacacgcg ccggctgagc cagatcatgc
    12241 cctgcaccgc gtagccgagc cccgacagga acaggtggat cttgcggctc gcggcgatcc
    12301 accgctcgac ccgcaggtcc cacgcgtcgc gctcgtacac cacgcgggcg aaccagtggt
    12361 tgacccggcg aatgacgtac accgcgcgca tgccgaggct ctggctcagg tgcccgaact
    12421 cgtgggcgag cacgccggtg aactgcggca gggtctgaca cgcgaccagc ggcaggccga
    12481 tcgtcagcac gaggtcgttt ccgaagaacg acaggacgcc gcggcgcagc gaggcgctgg
    12541 cattgaccgc gcaggtgacg acgatgcgag ccggccgcgg cgcgtgcacg gcgttgcaga
    12601 tcttctcgac gaaggcgaac agcagcggct cctcggcggg gtcgagcacg cgcgaggccg
    12661 gatccggcgc acgccgggcg agcagcggct tgagcaggaa gaacaccccc agcccgccga
    12721 tcacgaacgg cgcgacgtag gcgaaggcga ccagcaggcc cttttgcccc gccccgtcga
    12781 tcatcccggc gttgtgggtg gcgtgccaga ccatcaccca gaccagcgcg ccgatgatcg
    12841 ccaggtacac cagcggcagc agcagcatca gcaccgcgac cagcacgatc gccacgcgat
    12901 acagccacgt cgaggggacc ggccggatct ccccgcgaaa gcccgcgagc accgggtctg
    12961 cgagcacgga tgccggcacg gccgtcgcca tgcccgacga tatcagggcg tttgggccat
    13021 gtattcgcgg gaccgattaa aataggcggt ccagctatag gtgatccact cgcggtcggc
    13081 gtccgtcatc gggcggacca gcttgccggg gttgcccatc atcagctgcc cggtcggcac
    13141 gaccttgccg ggcgggacca acgttccggc ggcgacgaac gcccagtcct cgacgaccgc
    13201 gttgtcgagc aggcacgagc ccatcccgat caggcagtcg tcgccgatcg tgcagccgtg
    13261 caggatcacc cggtggccga cggtcacgcg cgccccgacc gtcgtgcgcg agcgcccctc
    13321 ggtcatgtgg atcaccgtgc cgtcctggat cgacgtgttg gcgccgatga cgatcgggcc
    13381 gtcgtcggcg cgcagcaccg cccccggcca gatgctcgcg cccgggccga cctcgacgtc
    13441 accgatcagc gtcgcactcg ggtgcacgta cgccgtcggg tcgacccgcg ggaacttgcc
    13501 gcgaaagccc tcgagcatct cacatcccga atacgaggaa ggcgatcacg ccgccggcga
    13561 ccagcagcag cacgagcacg atgatccacg aggagccgcc ctccttgctc gtcggcgcgg
    13621 gcgggcgtgc cgttcgcggg tccggcggga tcgtgcgctg cggctgctgc ttgcccgtcg
    13681 ccggcccgcc cggtgcaggc ttcgcgctcg agccaccgag gccgagctcc tcgtcgatcg
    13741 acatgtcccg gcgcggcatc ggctcgactg gcgtcggcga caggccgagc ttgttcggcg
    13801 tcaacggcac cttgccaggc accggcgtga acacgtcgac gtcgagcgga cgcgacgggt
    13861 cgacatcctg ccggccgctg gtgctcgcat tggggttact cgagccgctg gagctgccgc
    13921 gcgtcgcctc ggcgaccggc accaccggct cggacgcgag gccctcctgg gccggcttct
    13981 cgccctcgcc gagcgaccgg aagtgcagca tctcctcgtt gatgaggttg atgatcaggt
    14041 tgttgccgcc gggggagcgc tcctcctggg ccttcttctc ctgcgcgcgc ttgtcggcct
    14101 cgatctcctc gcggatcggc ttgcagtact cgatcaggtc gcgcgcgccg accttgaggc
    14161 ggttcgagaa caggaactgc agcagcgcgt cggcgaagtc gtcggcgtcg gcgtagcggt
    14221 cgttgatgtc cttggacagc gcccgcatga tcaccgcctc gagctcgggc gggaccgcgg
    14281 ggttgtacga gcgcacgctc ggcacctcgc aggcgcggac gagggcgatc gtctggtagt
    14341 cgttcttgcc gcggaacagc cggcggccgg tcaacatctc gaacgcgagg atgccggccg
    14401 agaacacgtc ggcccggtga tcgatctcct cgccgcgggc ggcctccggc gagaggtagc
    14461 tgtacttgcc cttgaccacg cccgggtcgg tcgactcgag ctgcgtcgtc gccttggcga
    14521 ggccgaagtc cgtcagcttg acctcgccgt tccacgacac caggatgttc ggcggcgaga
    14581 tgtcgcggtg gacgatgccg atcgggcggc ccgtctccga gtcggtcagc ttgtgcgcgt
    14641 acgccaggcc cttgaggatc tccgtcaaga tccaggtgac cagcggcacc ggcatcgccc
    14701 cgccgcgctt ggcctggtac tcgagcagcg ccttgaggtt cgtgccatgg acgaagtcca
    14761 tgacgatgaa gtaggtgttc tcgctctcgc cgatgtcgaa gaccgacacc acgttggcgt
    14821 gattgagtcg aaggctcagc cgcgcctcgt cgaggaacat gcggacgaac cgctggtcct
    14881 tgatcagccc cggaaggatc cgcttgatgg cgaccttctt cttgaagccc gccatcgact
    14941 cggcctcggc cacatagacc tcggccatgc cgcccgcgtc gagcttgtag agtggacggt
    15001 agcgttgaga ggtcatgagc tggggacggg gcgcgagtgt aacaggatcg tcggcccgga
    15061 aagcatccgc ggggaaacgg ggttcgcagg gctcagccgg ccctgggggc gggcgtacgc
    15121 gaccccgcgg cgtcgtgcgg cgggtcgtac tgggtcaaga tcaaacgcac gacaccgagc
    15181 cacaggtcgc tctcgatcat gagcgggatc cgtcgccctt cgctgctcac ccacacacgc
    15241 attttgtacg ctttcttgcc gggaattgcg gcgccaaacc ggtctacacg ggtgattacg
    15301 ccgtcgtagg cgacgacctg gtcctgcgtg atgcccagcg ccgccgccag cggcgggagg
    15361 tcgttggtgc gcgtcggtcc gcgtgcggtg atgtcgacgc gcagcagcgt gttgccgtcg
    15421 agcgcgtggg ccttcgcctt cgcgccgggc tcgaggtcga gcgaccgcac ccacgccatc
    15481 accgacagag gatcgaaggt gtcggcgggc aagcgcttgc gcgcgctcga ggtcttgccg
    15541 tccttgtcgt cggcgatctc cacgaagccg cggccgaaga aggtcgccac cgtctcgcgc
    15601 cgcttgtatg tcgtcgccat gccctcgcgc ttggtgatcg cgccggtcat caccggcgcg
    15661 cccgtctccg cgtcgaggat cgccgccatg tcgtagacga tcgtcgtcag cagcgccgcg
    15721 accccgctcg tgcgcgccga accctcgagg cggatgcggt ccggcgtgcc gttgggaccc
    15781 ggctccctcg ccacgaccgc cgcctcggcg atgccggtcg ggttgccgcc gaagtacacg
    15841 tcgaacttga agcgctcgcc cggccgcatc gtcgcgccga agtccgccgg tcgcggcacc
    15901 cacggcacac gatgcgtgcg gtcgacgaac tccggctccg cgagcgcgcc gctccacccg
    15961 agcagcggcg agcgcccggg cgccggccca cgcacgaccc ctggcggctc gagccggggc
    16021 agcctcgacg cgtcgtccgg ccagatcccg atctcgatct cgggcgtcgc cgcggccctg
    16081 ggctcgccgc gcggcgccgg tgtacgcggc tcctcggtcg ccgcagcagg agccgcgcgc
    16141 ggagccttct tcggcgcgat cgcaggcgtg ctgccaggcg tcgaagaaga tgtggccgga
    16201 gcagacgcgt cgtcgcccgt cgccggcgcc aaagccccgt cgacgaagta cgtgcctgcg
    16261 gccgacgcca ggagttttgc ggcaaggatc cagggcgtca tggtgtctcg cggccctcgc
    16321 ggccacgaag catgttagaa tgcccggccc ttgacgccaa ctggcgatcg caatgttgtc
    16381 cgagccagga acacctcccg atcccgtcga ccggcgtccc acgtcacagc tcgtggtcac
    16441 gcagggggac cccgcgggca tcggcccgga actgctcctg cgcctcggtg ccgcggatct
    16501 gctacgcaaa ggtgacgtcg tggtggcgga tcctgcgccc ctgcgccggc tcgccgacag
    16561 cctcggtgag tgggccgtgc gcggtctgac atctctcgag ccattgctcg atcccctgcc
    16621 gcacccgctc accagtcctt tcggacaggt gtccgcgctg atccgcggcg tcgacctcgt
    16681 gctcgcagat cccgcccgtc gcgcgctggt caccgcgccg atcgacaagg ccggctgtcg
    16741 cgcggagggc tttgagttcc ccggccacac cgagtacctc gccgcgcgcg cgggcgtcga
    16801 ggacttcgcg atgttgatgg ccggcccgcg cctgcgtgtc gtgctcgcaa cgatccacct
    16861 cgcgctgcgc gaggtgccgg agcgcctgtc gatcgccggc atcgtccgcg ccggtcgact
    16921 gctcgcccgc gcgatgcccc gctgggtccg cggtcgtccc gcgatcgtcg gcgtcctcgg
    16981 cctcaacccc cacgccggcg agcgcggcat gctcggcgac gaggagcgca cgatcgtcgg
    17041 gcccgccgtc gagctgctgc gccagtgggc cagcgagcac gcggtccccg tcgagttccg
    17101 cggaccgctg cccgccgaca ccgccttccc cgcccacgcc gagggccgcc tcgacggcgt
    17161 cgtcgccatg tatcacgacc agggcctcgg ccccttcaag ctcctgcaca tgcacgacgg
    17221 cgtcaacatg accctcggcc tgcccttcgt ccgcacctcg cccgaccacg gcaccgcccg
    17281 cgacatcgcc ggccgcggcg tcgccgaccc gtcgagcatg ttcgccgccg tcgccctcgc
    17341 ccgcggagga gaacctgtcc catgagcaag gtcgcaaaga ccagcaaggc cgccaccaag
    17401 acggccagca gcaagctcgc gcccgccccg gccaagccca ccgcggtcaa gaccgccgcc
    17461 gtggtgaaga ccacgaaggc cccgcgcagc acagccaagg ccgtgcccga ggccgccgag
    17521 gtcgtcgcgc tcccccccga gatcgccgtc gccgagacac ccgcccctgc gagcaccgcc
    17581 cccgcgagca ccacccccgc gaacgtcacg cccacgagca caccggcccc gatcgctgcc
    17641 gcctcgctca cccccgagcc cggcaccccc gagcccccgc ccgtccccgc gcttgccccg
    17701 ctggccccgc aggtccccgc gccgtgcatc cggatccgcg gcgcgcggac ccacaactta
    17761 cagagcatcg acctcgacct gccgcgcggc aagctcgtcg tcatcaccgg gccctcgggt
    17821 tcgggcaaga gcagcctcgc ctttcacacg atcttcgccg agggccagcg ccgctacgtc
    17881 gagagcctga gcgcctcggc gcggcagttc ctcgcgcagt taccaaaacc cgacgccgac
    17941 ctgatcgagg gcctcagccc tgcgatcgcg atcgagcaga gccctggcgg tcgcaacccg
    18001 cgctcgaccg tcggcacctc gacggaggtc tacgacttcc tgcgcctgct ctacgcgcgc
    18061 gtcggcgagg tctacagcta caagaccggc aagccgatcc gccggcacac gatcgaggac
    18121 atgctcgccg gcaccctcgc gctgccgccc ggcacgcgct tcagcgtgat cgccccggtc
    18181 gtgcgcggac agccgggcga ccacgccgag ctgctcgccg acctgcgccg tcagggcttc
    18241 tcgcgcgtgg cgatcgacga cgaggtgcgc gacctcgccg acacgatcac cctcgacccc
    18301 ggcaagcgcc acacgatcga ggtctacgtc gaccgcctgg tgatgaagga gggcgtgcgc
    18361 ggccgcctcg ccgactcctt cgaggtcgcg ctgcgcctca ccggcggcct ggtcaagatc
    18421 ctcacgatcg agggccagga gctgctgttc agcgagcaat acgccgactt cgaccacggc
    18481 ctcgcctacc cggagatcac gccgagcctg ttctccttca acagccccga cggcgcctgc
    18541 ccggagtgcg acggcctcgg ctcgcgccgc gtcttcgacc cgcgccgcct cgtccccaac
    18601 gagcatcttt ctttaaaaga gggcgcgctg cacgtgtgga cgcgccgcgg cgggcgggcc
    18661 agcacgcagg cgctgcaggc cctctcggag cactacaagt tcgacctgtt cgccccgtgg
    18721 atcaagctcc ccgacgaggt caagatcgtg ctgctgcagg gcagcggcga cgaggcgatc
    18781 ccggcgctcg gcaagaagcc gacccccttc gagggcgtga tcccctcgct cgagcgacgg
    18841 ctgcgcgagg ccgagaccac cgccggcgag tccgacgagg agggtggcac gctcgaggag
    18901 gtcgaggcct tgatgagcga ggtcgtgtgc acgagctgcg agggccagcg gctgcgccag
    18961 gaggcgcgca tggtcaagat cggcggccac aacatcagcg agatctccgc ctggccgatc
    19021 gagcagctcc tgcccttcct cgagcgcctc gagctcggtg aggagcgcaa cgaggtcgcc
    19081 gaggcgatcc tcgggcagtc acgccagcgc ctgcgcttca tgctcgaggt cggcctcggc
    19141 tacctgaccc tcgaccgcac gacgatgacc ctgtccggcg gcgaggccca gcggatccgc
    19201 ctcgccacgc aggtcggcgc cgcgctcgtc ggcgtcacct acatcctcga cgagccgagc
    19261 atcggcctgc atcaacgcga caacgaccga ctgatcgcgg cgctgatccg cctgcgcgac
    19321 ctcgggaata cagtcctggt cgtcgagcac gacgaggaca cgatccgcgc cgccgacttc
    19381 ctggtcgaca tgggccccgg cgccggcgtg cagggcgggc gtgtcgtcgc cgccggcccc
    19441 ttgcaagagg tgctcgccaa ccgtcgctcg ttgacggccg cctacctgac cggccgcatg
    19501 agcatcccgc tccctgaaaa ataccggaga tcgatcggcc cggcgctggt gatcagccac
    19561 gcgaccggcc acaacctcaa ggatgtcacc gtcaagatcc cgctcggcac cttcacctgt
    19621 gtcaccggcg tctctggctc gggcaaatcg tcgctgatca tcgacaccct gctgccggag
    19681 gccgtgcgca ggctcaatgg cggcagcggc ttcggcctcg cgcatcatgg catcaccggc
    19741 ctcgagaacc tcgacaaggt catccacgtc gaccagtccc cgatcggtcg ctcgccgcgg
    19801 tccaaccccg cgacctacac cggcctgttc gccgagctgc gcaccctgtt cgccaacctc
    19861 tcggagtcca aggtccgcgg ttacacgccg gcccgcttct cgttcaacgt caagggcggc
    19921 cgctgcgagt cgtgtcaggg cgacggcgtg cgccgcatcg agatgcactt cctcgcggac
    19981 gtgttcgtca cctgccgcgc ctgcaacggc cgccgctaca accgcgagac cctcgccgtc
    20041 acctaccgcg gcaagtcgat cgccgacgtg ctcgccatgt cggtcagcga cgcgtgcgac
    20101 ttcttcgcca actaccccgc gctgcgccaa aagctcgaga ccctgcgcga cgtcggcctc
    20161 ggctacgtca ctctcggcca gagcgccctg acgctgtccg gcggcgaggc ccagcggatc
    20221 aagctcgcca aggaactggc caagaagtcg accggcaaga ccctcttcat cctcgacgag
    20281 ccgaccaccg gcctgcactt cggcgacatc aagctcctct taaaagtgct ggatcggctc
    20341 gtcgacgagg gcaacaccgt cgtcgtcatc gagcacaacc tcgacgtgat caagtgcgcc
    20401 gaccacatca tcgacatcgg ccccgagggt ggcagccgtg gcggtgagct ggtcgccagc
    20461 ggcacgccgc cgcaggtcgc cagggtggcc gcgagcgcga ccggcaagta cctcgcgcgg
    20521 gtgctgcaag cccccggcgc gtgaacggcc cacgtggcag cgtcgccacg ggagcgatcg
    20581 gatcacgagg atctcgggga cgatcgctcg catcgatcgc ttgctcgtcg ggtccgccgc
    20641 tgccatcctg tgcccatgcg cttgccttat ttactgctga cgctcgcggc cctgaccgcc
    20701 gcgggtcgag ccgccgcgac ctcgtgcgga gatctaccgt gggccaacga atactcgagc
    20761 ccgctggaga acatcggcgt cgcggtgccg ttcgacgccc acccgtggcg gatcgacacg
    20821 tgcttcagcg ggccgcccag gacgccggta gattgcagct tcatcgatga cgagaccaag
    20881 gctgcgatcg ccgccgaggt caccgtgacc gataccgcgg cctgcagcct cgacctgccc
    20941 aacgacgccc ctgccagcg
//
LOCUS       DASWNJ010000786         3646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_155897, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000786 DASWNJ010000000
VERSION     DASWNJ010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155897"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="VGB85_13205"
     CDS             complement(<1..1273)
                     /locus_tag="VGB85_13205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0135043.1"
                     /translation="MTSLFIVEDDLHLRSALIRELEAHGFEVRAASSVDEALISIGQK
                     PVDVVLTDLRLDGQDGIDLLRRLPAVSRRTRVVLMSAYATARDHQIATELGAVTVLVK
                     PFTWDELLRTIQKAIDCETGFVGSIHGLSLVDMAQMFHLAQRSISVRVSQPGGSPSAI
                     HFRGGEIVHAEHRGLVGTPALRAILATPFGTIDTAPFAEDTETTISSNFDHLLLESLS
                     QLDEEQHDLQRIALRATGGFAGVEELQVRDDEPGALALSSSPAFSSSPSATATLLPER
                     PVRPIELAATMLEVPPVEAERRTIPVDDPIYHASQPPSALQPALSEACRGLMAAIDAA
                     VACTIVALENGALIGHHQTAGLAEPVGPILAAATHALFAGPALTRLAAVEVGPTGVEV
                     TQEAQLTAARHYLFARVLPDGRSAVALLTRKT"
     gene            complement(1270..2304)
                     /locus_tag="VGB85_13210"
     CDS             complement(1270..2304)
                     /locus_tag="VGB85_13210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0135044.1"
                     /translation="MSASDTTPVDPADPSPAWDGQALRLAEVAAPLALCDSHGRLLTL
                     TASARALLARVDVAGDALPASLWQVLCAAATGEAALWRPEGGALCLGCTRYDVGEQHH
                     LLLMREVSDKQRELSQRMHQQRLEATGSLVTAITHDIRGALASVIFNADVLATRGGAM
                     DPAAVRPFLAEILGGAERLKQIVDGLLDFARLRDQPQPFAPLEPVLQRVSAALRSKFR
                     DRSAELSITHATPALRASGNPLIIEQILGGLLQNAIEAGARHVCVHCEPTPDGRVRVR
                     VHDDGPGVPAELHGRIFEPFFTTHGAHPGLGLSTGREAARHIGGELAIDASEHGASFS
                     LYLRGGGGTA"
     gene            complement(2301..2663)
                     /locus_tag="VGB85_13215"
     CDS             complement(2301..2663)
                     /locus_tag="VGB85_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135045.1"
                     /translation="MGNAVNQVKQALEIEGALAAALVDGDSGMMLASAGDNSFDLEIA
                     AAGHTELLRAEHKTMRALGVTDTVSDILVSLGQQYHLLRPLRSAPSIFFYLALDRSRA
                     NLAMARHLLSVLEASTTL"
     gene            3023..>3646
                     /locus_tag="VGB85_13220"
     CDS             3023..>3646
                     /locus_tag="VGB85_13220"
                     /inference="COORDINATES: protein motif:HMM:NF024931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HEY0135046.1"
                     /translation="MLTRTYAACIAGVDATVVTVEADVGLGLPGLTIVGRASGALIEA
                     RERVRSAISHCGHKIHPRKQVVNLAPADERKDSPGIDLAVACALLASHEVVPWQRLEG
                     LMLWGELALDGTLRPAAGTLVIADCARRGGFTAIAVARDSADEASLIPGLTVYALDSL
                     PQLVAHLRGEAALAGHVRSELDADEVPTGEGDMADIRGLALARRALEV"
BASE COUNT          512 a         1150 c         1484 g          500 t
ORIGIN      
        1 tcgtcttgcg ggtcagcagc gcgacggcgc tgcggccgtc gggcaggacg cgggcgaaca
       61 ggtagtggcg cgccgcggtc agctgggcct cctgcgtgac ctcgacgccg gtcggcccga
      121 cctcgacggc ggcgaggcgc gtcaacgcgg gcccggcgaa cagcgcgtgg gtggcggcgg
      181 cgaggatcgg cccgaccggt tcggcgagcc ccgcggtctg gtgatgaccg atcaaggcgc
      241 cgttctcgag cgcgacgatc gtgcaggcga cggcggcgtc gatcgccgcc atgagcccgc
      301 ggcacgcctc cgacagcgcc ggctgcaacg ccgagggcgg ctgcgaggcg tggtagatcg
      361 ggtcgtcgac ggggatcgtg cggcgctcgg cctcgaccgg cgggacctcg agcatcgtcg
      421 ctgccagctc gatcggccgc acggggcgct cgggcagcag cgtggccgtc gcggatgggg
      481 acgatgagaa agctggggaa gatgagagag cgagcgcgcc cggctcgtcg tcgcggacct
      541 gcagctcctc gacgccggcg aagccgccgg tggcgcgcag ggcgatgcgt tgcaggtcgt
      601 gctgctcctc gtcgagctgg ctgaggctct cgagcaggag gtgatcgaag ttgctcgaga
      661 tcgtggtctc ggtgtcctcg gcgaacgggg cggtgtcgat cgtgccgaag ggtgtggcga
      721 ggatcgcacg cagggcaggg gtgccgacga ggccgcggtg ctcggcgtgg acgatctcgc
      781 cgccgcggaa gtggatcgcg ctcggggagc cgccgggctg gctgacgcgc acggagatcg
      841 agcgctgggc gaggtggaac atctgcgcca tgtcgacgag cgacaggccg tggatgctgc
      901 cgacgaagcc ggtctcacag tcgatcgcct tctggatcgt gcgcagcagc tcgtcccagg
      961 tgaacggctt gacgagcacg gtgacggcgc cgagctcggt ggcgatctga tgatcacgcg
     1021 cggtcgcgta ggcgctcatc aacacgaccc gcgtgcgccg cgagaccgca ggcaggcggc
     1081 gcagcaggtc gatgccgtcc tggccgtcga ggcgtagatc cgtcagcacg acgtcgaccg
     1141 gcttctgccc gatcgagatc agcgcctcgt cgacggagct ggccgcgcgg acctcgaagc
     1201 cgtgcgcctc gagctcgcgg atcagggcgc tgcgcaggtg gaggtcgtcc tcgacgatga
     1261 acagggaggt cacgccgtac ctccgccgcc gcggagatac aggctaaagc tggcgccgtg
     1321 ctccgatgcg tcgatcgcca gctcgccgcc gatgtgacgg gccgcctcgc gcccggtcga
     1381 caggccgagg ccggggtggg cgccgtgggt ggtgaagaag ggctcgaaga tgcgaccgtg
     1441 cagctcggcg ggcacgccgg ggccgtcgtc gtggacgcgg acgcggacgc ggccgtcggg
     1501 ggtcggttcg cagtgcacac agacatgacg cgcgccggcc tcgatcgcgt tttgtaacaa
     1561 gccgccgagg atctgctcga tgatcagcgg gttgcccgag gcgcgcaggg ccggcgtggc
     1621 gtgcgtgatg ctgagctcgg cgcttcggtc gcggaacttg gaccgcaggg ccgccgagac
     1681 gcgctgcagc accggctcca gcggtgcgaa gggctggggt tggtcgcgca ggcgggcgaa
     1741 gtcgagcagg ccgtcgacga tctgcttgag acgctcggcg ccgccgagga tctccgcgag
     1801 gaaggggcgc acggcggcgg ggtccatcgc gcctccgcga gtggcgagca cgtcggcgtt
     1861 gaagatcacg ctggcgagcg cgccgcggat atcgtgggtg atcgcggtga cgaggctgcc
     1921 ggtcgcctcg aggcgctgct gatgcatgcg ctgcgagagc tcgcgctgct tgtcggagac
     1981 ctcgcgcatc agcagcaggt ggtgctgctc gccgacgtcg tagcgggtgc agccgaggca
     2041 cagcgcgccg ccctccgggc gccacagggc ggcctcgccg gtcgccgcgg cgcacaggac
     2101 ctgccacagg ctcgcgggca acgcgtcgcc ggcgacgtcg acgcgggcga gcagggcgcg
     2161 cgccgaggcg gtgagcgtca gcagccggcc atggctgtca cacagcgcga gcggggcggc
     2221 gacctcggcg aggcgcaggg cctgaccgtc ccacgcaggc gaggggtcgg ctgggtcgac
     2281 aggcgtggtg tcgctggcgc tcacagggtg gtgctggcct cgaggacaga caggagatgg
     2341 cgggccatcg ccaggttggc gcgcgagcgg tcgagggcga gatagaagaa gatcgagggc
     2401 gcggagcgca gcgggcggag caggtgatac tgctgcccga ggctgacgag gatgtcgctg
     2461 acggtgtcgg tgacgccgag ggcgcgcatg gtcttgtgct cggcgcgcag cagctcggtg
     2521 tgaccggcgg cggcgatctc gaggtcgaag gagttgtcac cggcggaggc gagcatcatg
     2581 ccgctgtcgc cgtcgacgag cgcggctgcc agcgcgccct cgatctccag ggcttgcttg
     2641 acctgattga ctgcatttcc catgaagtgg acttcctcgc tgaaaatggg tctgcgctgc
     2701 gggacaggtg agccggctcc ggggccaagg tggggctcat gttctggccc acttgcgggc
     2761 tcgccgtgcg ggtccccgag gcaggccttt gggacaggtc gcgcgcgaac gacctcgctg
     2821 gcacgataac ggatcggccc ggggctcgca acgcgcgcgg gcgcgcgggg ggttcgttgc
     2881 atgtcgacat ccgcgtatgg gtcgcgtgtc ccgcgggcgg gacgcctgtc tcgcgggcgg
     2941 gacggcggcg cggcgagcgt gcgcgcaagt gcgtgtaaca ccacgcgacg gcatcgcgca
     3001 cgcagcttgt aaggcgaggc gagtgctcac gagaacctat gcggcgtgca tcgccggcgt
     3061 cgacgcgacc gtggtgacgg tcgaggcgga cgtcgggctc gggctcccgg ggctgacgat
     3121 cgtcggtcgc gcgagcggtg cgttgatcga ggcgcgcgag cgggtgagga gcgcgatcag
     3181 ccactgcggg cacaagatcc acccgcgcaa gcaggtcgtg aacctggcgc cggccgacga
     3241 gcgcaaggac agcccgggga tcgacctcgc ggtcgcgtgc gcgctgcttg ccagccacga
     3301 ggtcgtgccg tggcagcgcc tcgagggcct gatgctgtgg ggcgagctgg cgctcgatgg
     3361 cacgctgcgg ccggccgccg gcactctggt gatcgccgac tgtgcgcggc gcggcgggtt
     3421 cacggcgatc gcggtggcga gagacagcgc cgacgaggcc tcgctgatcc ccgggctgac
     3481 ggtgtacgcg ctggactcgc tgccgcagct cgtcgcgcac ctgcgcggcg aggcggcgct
     3541 ggcgggccac gtgcgaagcg agctcgacgc ggacgaggtg ccgaccggcg agggcgacat
     3601 ggcggacatc cgcgggctgg cgctggcgag gcgggcgctc gaggtg
//
LOCUS       DASWNJ010000787         3255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_684617, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000787 DASWNJ010000000
VERSION     DASWNJ010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684617"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /locus_tag="VGB85_13225"
     CDS             <1..1262
                     /locus_tag="VGB85_13225"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY0135047.1"
                     /translation="TGLGDAPGLHHYSPLLHGLERRADLTPALWSALTTGLPQPCLLV
                     GLTTIAWREAWKYGERAFRYCQHDLGHAIAAVDIAAAMFGWRALELPRPGDAELATLL
                     GLRRDSGPEPEVPEALLVLVPASSDVSAIGEWTLAADALAELARLPLRGVANRLSEAH
                     QPWPVIDEVTRATHRTAAAVPDQRSPARPPVILPERETPPASTVIRGRRSAVAMDGRT
                     GITRAGFYDMCRRTAHDAPPLRALAGPARIDLAIFVHRVEGLDPGLYLLRRGTTRGER
                     WRLAGDPEFTWTRPEGCPEDLDLSLLRIGDGRQLAAAISCGQDIAADGCFALAMLADM
                     QVLAAAPWLYRRLYWEAGAIGQILYLEAEALGIAATGIGCFFDDRMHALLGLRDGEFQ
                     SLYHFTVGGRVDDTRLRTLDPYEHLDR"
     gene            complement(1265..1951)
                     /locus_tag="VGB85_13230"
     CDS             complement(1265..1951)
                     /locus_tag="VGB85_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135048.1"
                     /translation="MAFRAFQGPDFNFDSLTVEGGPLATQLAAWSARAVAAGHVPCVY
                     LTAGWCPPSVKLEKSLADPAMALALRDVAAATLDVDAWGDELTAAGFSARSIPVFYAL
                     DDEGRPSGQSITGAAWGENTPENMGPPLGRFFDALRDAQAPERFKQSRDPSPVTPDTS
                     VTPAGPPEPRSRAARTTMVIAALVLLGLIAFIKVYLDGEQQHEQDTTERDSRIQQDIQ
                     RSIQDSLKKQ"
     gene            2122..3099
                     /locus_tag="VGB85_13235"
     CDS             2122..3099
                     /locus_tag="VGB85_13235"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0135049.1"
                     /translation="MSLAALPACGGNDTAGTASATGSSSSGSGGVETTTGQPTTSATD
                     PSTTEAPPTTGMSEAMTSQGPTADASTSTTVEPTTDATTTSDTGDTGDTGDTTAADPF
                     CGDGQIDAGEQCDDGEANRPGQPCNAMCLANTCGDGDQGPTEECDDGADNADNASCKP
                     DCTNNICGDGAVGPGEGCDDGNQDDADECGNDCALLTCGDGKVAPTEQCDDGNKENSD
                     ACTVTCTNAVCSDGFVQAVLGEQCDDGVDNADNAACTTLCASNVCGDGALYNTGSGTE
                     ECDDGADNGPGKTCNAQCDLNTCGDGDQGPGEECVDANNSPADGCSATC"
BASE COUNT          485 a         1190 c         1158 g          422 t
ORIGIN      
        1 tcaccggcct cggcgacgca cccggcctgc atcactacag cccgctgctg cacggcctcg
       61 agcgccgcgc cgacctcacg cccgcgctgt ggtccgcgct gacgaccggc ctgccgcagc
      121 cgtgcctgct cgtcggcctc accacgatcg cctggcgcga ggcgtggaag tacggcgagc
      181 gggcgtttcg ctactgccag cacgacctcg gccacgcgat cgcagcggtc gacatcgcgg
      241 ccgcgatgtt cggctggcgc gcgctcgagc tgccgcgccc gggcgacgcc gagctggcga
      301 ccctgctcgg cctgcgccgc gacagcgggc ccgagcccga ggtccccgag gcgctgctcg
      361 tgctcgtgcc cgcgtcgtcc gatgtctccg cgatcggcga gtggacgctc gccgcggacg
      421 cgctcgccga gctcgcccgg ctcccgctgc gtggcgtggc gaaccgcctc agcgaggcgc
      481 atcagccgtg gcccgtgatc gacgaggtca cccgcgcgac gcatcgtact gcggcggccg
      541 tgcccgacca gcgcagcccc gcgcgtcctc ccgtcatcct ccccgagcgc gagaccccgc
      601 cggcctcgac cgtgatccgc ggccgtcgca gcgcggtcgc gatggacggt cgcaccggga
      661 tcacgcgggc tggcttttac gacatgtgtc gaaggacagc ccacgatgct ccgccgctgc
      721 gcgcgctggc cggcccggcg cggatcgacc tggcgatctt cgtgcaccgc gtcgagggcc
      781 tcgaccccgg gctgtacctg ctgcgccggg gcaccacccg cggcgagcgc tggcggctcg
      841 ctggcgatcc ggaattcacg tggacgcgcc cggagggctg tccggaggac ctcgacctct
      901 cgctgctgcg gatcggcgac ggccgccagc tcgccgctgc gatctcgtgc ggccaggaca
      961 tcgccgccga cggctgcttc gcgctggcga tgctcgccga catgcaggtc ctcgcggcgg
     1021 cgccgtggct gtatcgccgg ctgtactggg aggccggcgc gatcgggcag atcctctacc
     1081 tcgaggccga ggcgctcggg atcgccgcga ccggcatcgg ctgcttcttc gacgaccgca
     1141 tgcacgcgct gctcggcctg cgcgacggcg aattccagag cctttatcat ttcaccgtcg
     1201 gcggccgcgt cgacgacacc cgcctgcgca cgctcgaccc ctacgagcac ctcgaccgct
     1261 gagcctactg ctttttcaag ctgtcctgga tcgacctctg gatatcctgc tggatccggc
     1321 tgtcgcgctc cgtcgtgtcc tgctcgtgct gctgctcgcc gtcgaggtag accttgataa
     1381 aggcgatgag gccgagcagc accagcgcgg ctatcaccat cgtggtccgc gccgcgcgtg
     1441 accgcggctc cgggggaccg gcgggcgtca cggacgtgtc gggcgtgacg ggcgacgggt
     1501 cgcgcgactg cttgaaccgc tccggcgcct gcgcgtcgcg cagcgcgtcg aagaagcgcc
     1561 ccagcggcgg ccccatgttc tcgggcgtgt tctcccccca cgccgcgccg gtgatgctct
     1621 gcccgctcgg ccggccctcg tcgtcgagcg cgtagaacac cgggatgctg cgcgccgaga
     1681 agccggccgc cgtcagctcg tcgccccacg cgtcgacgtc gagggtcgcg gccgcgacgt
     1741 cacgcagcgc cagcgccatc gccgggtctg ccagcgactt ctcgagcttg acgctcggcg
     1801 ggcaccagcc cgcggtcagg tacacgcagg gcacgtggcc cgcggcgacg gcccgcgcgg
     1861 accaggcggc cagctgtgtg gcgagcggcc cgccctcgac ggtgagcgag tcgaagttga
     1921 agtccgggcc ctgaaacgcg cgaaatgcca tgctccggcc actctacagc aagcttcgct
     1981 cgccaacgca ccgcatgggg gccggcggcc accccgctgt tgtctgttgc ccccacctcc
     2041 gatacggtgc cagcgggcga ccgatgcggt cgaaggggtg gggttatgcg gggcgatcga
     2101 tggatgcgtg gtggtttgtt catgtcgctg gctgcgttgc cggcctgcgg tggcaatgac
     2161 acggcgggta cggcgagcgc gaccggcagc tcgagctcgg gcagcggcgg ggtcgagacg
     2221 acgacggggc agccgacgac gagcgcgacc gacccgtcga ccaccgaggc gccgccgacg
     2281 accggcatga gcgaggcgat gacctcgcag ggtccgacgg ccgacgcgag cacgagcacc
     2341 accgtcgagc cgacgaccga cgcgacgacg accagcgaca cgggcgacac cggtgacacc
     2401 ggcgacacga ccgcggccga cccgttctgc ggcgacggcc agatcgacgc tggcgagcag
     2461 tgtgacgacg gcgaggcgaa ccgccccggc cagccgtgca acgcgatgtg cctcgccaac
     2521 acctgcggcg acggtgacca gggtcccacc gaggagtgtg acgacggggc cgacaacgcc
     2581 gacaacgcca gctgcaagcc cgactgcacc aacaacatct gtggcgacgg cgccgtcggc
     2641 cctggcgagg gctgcgacga cggcaaccag gacgacgccg acgagtgcgg caacgactgc
     2701 gcgctgctga cctgcggcga cggcaaggtc gcgccgaccg agcagtgtga cgacggcaac
     2761 aaggagaaca gcgacgcctg cacggtgacc tgcaccaacg cggtgtgcag cgacggcttc
     2821 gtgcaggcgg tgctcggtga gcagtgcgac gacggggtcg acaacgccga caacgccgcg
     2881 tgcacgaccc tgtgtgcgag caacgtgtgc ggcgacggtg cgctgtacaa taccggcagt
     2941 ggcacggagg agtgtgacga cggcgcggac aacggcccgg gcaagacctg caacgcgcag
     3001 tgcgatctca acacctgtgg cgacggcgac cagggtccgg gcgaggagtg cgtcgacgcc
     3061 aacaacagcc cggccgacgg ctgctcggcg acctgctagc tcgagcagtg cggtaaccag
     3121 accgtcgatc ccggcgagga gtgcgacgac ggcaacaacg gcgacgacga cgacggctgc
     3181 accgacgcct gcaagggccc cgtgtgcggc gacggcctgt tgcagcccaa caacggcgag
     3241 cagtgcgacg ccggc
//
LOCUS       DASWNJ010000788         5758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_545307, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000788 DASWNJ010000000
VERSION     DASWNJ010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545307"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1609
                     /locus_tag="VGB85_13240"
     CDS             <1..1609
                     /locus_tag="VGB85_13240"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEY0135050.1"
                     /translation="HAVAGAWYRYVRETRKIRPGRIFLLRDAEATPAAIAKALERAHA
                     TVGKRGTVWFVFVGHVSGTLPGKYGALWLRAGDGTPATLQQHAFSAAQVLSRLGHGLH
                     PRAIAVFDGCLAGGAQALPSGTETPELPPWTNRGAIDDIPVIPEELLRGPSTIGTEMM
                     IAQIKTQIDFARGRREPADVAVFSSGHGSGCVEDLPGTRFPALSYLLLGGLRGWADRN
                     KDGNVSAVELITQTTEMLRAAHHGSPGPAPAPSSFSADIVLARDVREPPPDVRGLLPP
                     DIARTFPVATLLKEPVLWSRDLMIRFERGRFKMGCWWRGDTDCEKDEKPAHLVWVSRF
                     QLDPQEVTQADYAACVAGGSCDAVDPARCFVWTGNRFIKGAAMPEPLLRPQHPVVCVS
                     WFQAQRYCAAHGKRLATEAEWERAAAGLTRRRFPWGEKNPDCTRAHFDGCGEHTRPVG
                     QRPAGATPEGVHDMGGNAAEWVHDWYARDIYKTIRDRDPIGGQTGVVRVVRGGSYYDA
                     PVALRSSYRYGLNPMSSFSTVGFRCAR"
     gene            1731..2705
                     /locus_tag="VGB85_13245"
     CDS             1731..2705
                     /locus_tag="VGB85_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135051.1"
                     /translation="MVSISRKFVIAYVGLLLGCPQADKQNTAGEPDTDPGTVGSGDTT
                     AGTGTGIGATEPGTGTTEPGTTEPGTTTPDTGTTAPDTGTTAPGEDTGPDTDTDEDTG
                     PSNEDVPQECMIGAKNTAPFVTGDFGDLDETCVVLASAIVNEVLEMTLECPIFAMGNG
                     GKPIHMKVENVPLPAAPPQVGESLGVVYQIPDFEGFFDYAQPELLFLHRDGALLHAVI
                     HGYFLTDEDLALVDTHALPLAVALVQGACVHVGTGWSGGDGTGCVYEAVAQLKLTGDA
                     ELLLSPGQTGEVAAGGHTYVADVREAYHRTECVDQAENIYYTMAIARK"
     gene            complement(2721..3158)
                     /locus_tag="VGB85_13250"
     CDS             complement(2721..3158)
                     /locus_tag="VGB85_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135052.1"
                     /translation="MNVDVLAQACAREARSLVADRDASLAWAGAAIACYARTSRRRSA
                     GFDRQGSEVPLFGLICLMISRWGPETGSEARDPAPVIEWVQQGIDILVDPQIFRASLA
                     DAGSERREHAVLLEERLEIARSLHALGLLPSSMIPWLHSAGLF"
     gene            complement(3484..3735)
                     /locus_tag="VGB85_13255"
     CDS             complement(3484..3735)
                     /locus_tag="VGB85_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135053.1"
                     /translation="MPRPRSEIDLQQIAERLADGEPMAVVSREFELTQSMIHRRVRSF
                     RQELADHETSPARKLHWLEKATRVQVATAWLKLPREVED"
     gene            3914..4141
                     /locus_tag="VGB85_13260"
     CDS             3914..4141
                     /locus_tag="VGB85_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135054.1"
                     /translation="MTRPQQHDDITDSECLGDYPSLEVFVRATLEPLLPAELRWLLDC
                     LDLERVLRAMAADGRDRLRLERGRVWLDHLP"
     gene            complement(4531..>5758)
                     /locus_tag="VGB85_13265"
     CDS             complement(4531..>5758)
                     /locus_tag="VGB85_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135055.1"
                     /translation="FGARRALTMFALFALALPSWSCQRGPATLLVTPPEYAPAGQSKC
                     DIIASPTRPLVVEWPSADRASLEARLASGLVAVRAAGCEIEVLRQCVVPGSYLYRALT
                     RKNDQVLITDVDDLYVQMPLGAARLASKLARSGKLDVRMALVGMLEAPTGRIAADTLS
                     GDCDGATHVIAGVQLGAFQFFAGGSGEMGAGLVVGVAGLGGRSEASRELLSADGDPTR
                     CDGSASIDLRPPEGCGAVIRIELTPLKAAPPPVVAASCPSGSTMNDGRCVISQTYCPD
                     GTQPLDGRCMTMHRAWTEPPPRVEPPPPAPAASPAVAPTPTAGRAYGGGPSSVEEDPQ
                     PALCRDLCEQMLTCTAKWQGWTSPANELVTQQLQLCGKKCDTTPLTADELAKLDRCLG
                     RGCDVLEQCMPSRNRR"
BASE COUNT          928 a         2088 c         1879 g          863 t
ORIGIN      
        1 gcacgcggtc gccggcgcct ggtaccgcta cgtccgcgag acgcggaaga tccgcccggg
       61 gcgcatcttc ctgctgcgcg acgccgaggc gacgcccgcc gcgatcgcaa aggccctcga
      121 gcgtgcgcac gcgaccgtcg gcaagcgcgg caccgtgtgg ttcgtgttcg tcggccacgt
      181 cagcggcacc ctgcccggca agtacggcgc gctgtggcta cgagccggcg acggcacccc
      241 ggcgacgctg cagcagcacg ccttctcggc ggcgcaggtg ctgtcgcgcc tcgggcacgg
      301 cctgcacccg cgcgcgatcg cggtgttcga cggctgcctc gccggcggcg cgcaggccct
      361 gccctccggc accgagacgc ccgagctgcc gccgtggacc aacagaggcg ccatcgacga
      421 catccccgtg atccccgagg agctgctgcg cgggccctcg acgatcggca ccgagatgat
      481 gatcgcgcag atcaagacgc agatcgactt cgcccgcggg cgccgcgagc ccgccgacgt
      541 cgccgtattc tcgtcgggcc acggatccgg ctgcgtcgag gacctgccgg gcacccgctt
      601 tccggcgctc tcgtatctgc tgctcggcgg cctgcgcggg tgggccgatc gtaacaaaga
      661 cggcaacgtc agcgcggtcg agctgatcac gcagacgacc gaaatgctgc gcgccgccca
      721 tcacggcagc cccggccctg ccccggcccc gagctcgttt agcgccgaca tcgtgctcgc
      781 ccgcgacgtc cgcgagccgc cgcccgacgt gcgaggcctg ctcccgccgg acatcgcccg
      841 caccttcccg gtcgcgactt tattaaaaga gccggtgctg tggagccgtg acctgatgat
      901 ccggttcgag cgcgggcgtt ttaagatggg ctgctggtgg cgcggcgaca ccgactgcga
      961 gaaggacgag aagcccgcgc atctggtctg ggtctcgcgc ttccagctcg acccgcagga
     1021 ggtcacgcag gccgactacg cggcctgcgt cgccgggggc agctgtgacg cggtcgaccc
     1081 ggcccgctgc ttcgtgtgga ccggcaaccg ctttatcaaa ggcgcagcga tgccggagcc
     1141 gctgctgcgc ccgcagcacc cggtcgtgtg cgtcagctgg ttccaggccc agcgctactg
     1201 cgccgcgcac ggcaagcgtc tcgccaccga ggccgagtgg gagcgcgcgg ccgctggcct
     1261 gacgcggcgc aggttcccgt ggggcgagaa gaacccggac tgcacgcgcg cgcacttcga
     1321 cggctgcggc gagcacacgc gtccggtcgg ccagcgaccc gcgggtgcga ccccggaggg
     1381 cgtgcacgac atgggcggca acgcggccga gtgggtccac gactggtacg cccgcgacat
     1441 ttacaagacg atccgcgacc gcgacccgat cggcggccag accggcgtcg tccgcgtcgt
     1501 ccgcggcggc agttattatg atgccccggt cgccctgcga tcgtcgtatc gctacggcct
     1561 caacccgatg tcgagcttta gcaccgtcgg ctttcgctgc gcccgctgaa gatcgaccct
     1621 ggacccacga cggtcgtgtc gcgcccgggc ggcgaagtca tgtcgcgagc gggagatcgt
     1681 caatatgcac gtgagagcat cgctggcatg ctgcttgctt ttgaacaaac gtggtttcca
     1741 tttcgcgcaa attcgtaatt gcatatgtcg gtcttttact cggctgcccg caggccgaca
     1801 aacagaacac ggcaggtgag ccggacaccg accccggcac ggtcggcagc ggcgacacca
     1861 ccgctggcac cggcaccggt atcggcgcca cagagccggg caccggcacc accgagccgg
     1921 gcaccaccga gccgggcacc accacccctg acaccggcac caccgcccct gacaccggca
     1981 ccaccgcccc cggcgaggac accggccccg acaccgacac cgacgaggac accggcccca
     2041 gcaacgagga tgtgccgcag gaatgcatga tcggcgcgaa gaacacggcg cccttcgtga
     2101 ccggcgactt tggagacctc gacgagacct gcgtcgtgct cgccagcgcg atcgtgaacg
     2161 aggtcctcga gatgaccctc gagtgcccga tcttcgccat gggcaacggc ggcaagccga
     2221 tccacatgaa ggtggagaac gtgccgctgc cggccgcgcc gccgcaggtc ggtgagagcc
     2281 tcggcgtcgt gtaccagatc cccgacttcg agggtttctt cgactatgcc cagcccgagc
     2341 tgctgttcct gcaccgtgac ggcgccctgc tgcacgcggt gatccacggc tacttcctga
     2401 ccgacgagga cctcgcgctc gtcgacaccc acgcgctgcc cctggcagtc gcgctcgtcc
     2461 agggcgcctg cgtgcacgtc gggaccggct ggagcggcgg cgacggcacc gggtgtgtct
     2521 acgaggccgt cgcgcagctc aagctcaccg gcgacgccga gctgctgctg tcaccgggac
     2581 agaccggcga ggtcgcggcc ggcggccaca cctacgtcgc cgacgtccgc gaggcctatc
     2641 accgcacgga gtgcgtcgac caggccgaga acatttatta cacgatggcg atcgcccgca
     2701 agtgaccatc gcggcttgtt ttagaacagg cccgcgctgt gcagccatgg gatcatcgac
     2761 gacggcagga gcccgagcgc gtgcaacgag cgtgcgatct ccagacgttc ctcgagaagc
     2821 acggcatgtt ctcgccgctc ggagcccgcg tccgcgagcg acgcgcggaa gatctgcggg
     2881 tcgacgagaa tgtcgatccc ctgctggacc cattcgatga caggggccgg gtctcgggcc
     2941 tcgctgccgg tctcgggacc ccatcgcgag atcatcaggc agatcaagcc gaacagcggg
     3001 acctcgctgc cctggcggtc aaagccggcc gaacgacgcc ggctcgtccg tgcgtaacac
     3061 gcgatggccg cgccggccca ggcgagcgac gcatcccggt ccgccacgag cgagcgcgcc
     3121 tcgcgggcgc acgcctgggc gagcacgtcg acgttcaagg tgccatcatc gactgcgagc
     3181 gccgcacgaa ccgccgcgtg tcctcgttcg aacaattcat actcgtcgct catatcggca
     3241 tcctcccggg cacggctcgt cgagaccttc gtcagagtac gtcgagcaac gccacggctt
     3301 cggactggcg gcagagcttc gcggcgatgt caccgtgcct cgtgtccgtc gcaccagctg
     3361 acccgccgtc ttacctgcag cgtcgtccgc acctcgacga gtacgcggac cccgtgattc
     3421 cagtggtcga cggcctggcg tcggctcgat tgtcgagcac gccattttcg tcctttctcg
     3481 cggtcaatcc tcgacttcac gcggcagctt caaccaggcc gtagcgacct gaactcgggt
     3541 cgccttctcc agccagtgga gcttgcgagc cggggaggtc tcgtgatcgg ccaactcctg
     3601 gcggaagctc cggacccgtc gatggatcat cgattgggtc agctcgaact cgcgtgagac
     3661 gacagccatc ggctccccat cggcgagacg ctcagcgatc tgctggaggt cgatctcgct
     3721 ccggggacgc ggcaccggcg gactctagcg gcgtaaatcg gtgatatcaa tcgactttta
     3781 aattcgaatt gatccgtcga aaagagcctg ctagatcatt ttcgatgaat tccgtcgaaa
     3841 tattgaccgc tcctccatct ccccgggcca tgggtaacca gggcgatcgt cctgctcccc
     3901 gatggggaga tggatgaccc gcccgcagca gcacgatgac atcaccgaca gcgagtgctt
     3961 gggcgactac ccgagcctcg aggtcttcgt ccgcgccacg ctcgagcccc tgctcccggc
     4021 cgagctgcgc tggctgctcg actgcctcga cctcgagcgc gtcctccggg ccatggccgc
     4081 cgacggccgc gaccggctcc gcctcgagcg cggccgggtc tggctcgacc acctgccgta
     4141 aaggtcacca tcgcgccgtg ctatgcgtag ctgccagggc agcgcgcata caccgaccca
     4201 caaacggcaa gccaccgcga aaagctcgag cagaacgtgc cgtcgggaca gccaggagct
     4261 tcgagagccc ccgagcatag cgaggcgcga cgaagctcgc cacggccacc gtggtcacca
     4321 tcgcaacaaa tggcccctgc tgctgatctt gaggtatcga ccgagcatgt cagcgccgag
     4381 caacgcagta aaaaatggcg cagatgggcc gcctgtcgca tcacgccgat atagcgcgct
     4441 tctgtcgccg tgatcgccct gtgcccgtcg catcgccgcc gcgacgcaca ttgcctgcgg
     4501 cgctccgacg agctttgcgc ggcccgcttc tcagcgtcta ttcctggagg gcatgcattg
     4561 ctcgaggacg tcacatcccc ggccgagaca gcggtcgagc ttggcgagct cgtcggccgt
     4621 caaaggagtc gtgtcacatt tcttcccgca cagttgcagt tgctgtgtca cgagctcgtt
     4681 cgccgggctc gtccaacctt gccacttcgc cgtgcaggtc aacatctgct cgcagagatc
     4741 gcggcacaaa gcgggctgcg ggtcctcctc cacggaggac gggccgccgc catacgctcg
     4801 accggccgtc ggagtcggcg ccacggcggg gctcgctgcc ggagctggcg gcggcggctc
     4861 cacccgcggc ggcggctccg tccatgccct gtgcatcgtc atacatcgac cgtcgagggg
     4921 ctgcgtcccg tccgggcaat acgtctgcga gatcacgcag cgcccgtcat tcatggtcga
     4981 tccggacgga cagctcgccg cgaccaccgg cggcggcgcg gccttcaggg gcgtcagctc
     5041 gatgcggatc accgcgccgc agccctcggg cggccggagg tcgatggagg ccgatccgtc
     5101 gcagcgcgtc ggatcaccgt cggcgctcag cagctcccgg ctggcctcgc tgcgcccacc
     5161 gagccccgcc acgccgacca cgaggccggc acccatctcg ccgctgccgc cggcaaagaa
     5221 ctggaacgcg cccagctgga cccccgcgat cacgtgggtc gccccgtcac agtcgccgct
     5281 gagcgtgtcc gccgcgatac gcccggtcgg ggcctcgagc atgccgacga gcgccatacg
     5341 cacgtcgagc ttgccgctgc gcgccagctt gctcgcgagt cgcgccgcgc cgagtggcat
     5401 ctgcacataa aggtcgtcga cgtcggtaat gaggacctgg tcgtttttgc gcgtcagggc
     5461 gcggtacaaa tagctgcccg ggaccacgca ctggcgcagg acttcgatct cgcagcccgc
     5521 cgcgcgcacg gccaccagcc cactcgccag ccgggcctcg agtgaggcgc ggtcggccga
     5581 gggccactcg accaccagcg gccgcgtcgg gctggcgatg atgtcgcatt tactctgccc
     5641 cgccggcgcg tactcgggcg gcgtcaccag cagcgtcgcc gggccgcgct ggcagctcca
     5701 cgacggcagg gccagggcga acagcgcgaa cattgtcagg gcgcgccggg ctccgaac
//
LOCUS       DASWNJ010000789         2654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_50310, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000789 DASWNJ010000000
VERSION     DASWNJ010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50310"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1400)
                     /locus_tag="VGB85_13270"
     CDS             complement(<1..1400)
                     /locus_tag="VGB85_13270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0135056.1"
                     /translation="MTPAQEQTAELSERTGELLLSVRGLCVDRPDGSPLLVDVAIDLR
                     AREVVAVFGASGAGKSTLTTALHDPDSLRDRGFAVRTGPSVVATAVGIVPQRGALFDH
                     LSAADNIALALRNAEPPAPATVEAVAAWIERVDLPRAWGERGRAVAQVSGGEAQRLAV
                     ARTLAGGRRILLLDEPSVGLDPHRITLLAATLRRQIDEGEAGALVVTHDLGFAAALAD
                     RFIYMDPALRRPVELDVPAARSPAEVAKVEQALATAVRERLSRETASHGSSRAAQVGL
                     RRLVRDWLAPFAVAARVLAEIPAALRRPRDLAEVTRVTLSQALLRPAPFFIIVSLLVG
                     CTLLYIFHRALGGGGLPVRPDRVFSLIGSMHVLALAPPLSAILFAATSASAITAWLGG
                     MSLTRQTMALRGLGISEARYLWVPAWLVLAGAYVVLAAVFTAGMIAGGVAYLELGVPA
                     AGDALALVTADIVDPPA"
     gene            complement(1397..2224)
                     /locus_tag="VGB85_13275"
     CDS             complement(1397..2224)
                     /locus_tag="VGB85_13275"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY0135057.1"
                     /translation="MRVDLHCHSTHSDGSCSADEVATRAGRQGVELFCLTDHDSLAGF
                     AATQAALPGVTVLRGVELSCKHDSRTIHLLIYGVKEGPGADALEERLVGVQDDRRARL
                     RAIVGRLAELGIHLDVEQILRETAGRTPGRPDIARALVAVKAVRSMREAFERYLHDNG
                     PADVAFARITLAEGLALGRAAGARMSLAHPHTLRSAALVADIYRRHRADGLDGIEALY
                     GRYAHAEREVWLRMAKQMDLVVTGGSDFHGEAMPEVTQPGIELHEPHATRLRAWLGV"
     gene            complement(2277..>2654)
                     /locus_tag="VGB85_13280"
     CDS             complement(2277..>2654)
                     /locus_tag="VGB85_13280"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135058.1"
                     /translation="PPTAEMLAAVRAEALAAFQASMPVTPHPVLHALAGTATTTAALL
                     LGLPVYDRIKVDGSRFTVAQVQALRDALASETLAQRCERPGLERGRADVIVAGVTILL
                     AALEHCGADTLVVRDRGLRYALV"
BASE COUNT          370 a         1032 c          944 g          308 t
ORIGIN      
        1 gccggagggt cgacgatgtc cgccgtcacc agcgcgagcg cgtcgccggc cgcgggcacg
       61 ccgagctcga ggtacgcgac gccgcccgcg atcatgcccg ccgtgaacac cgccgcgagc
      121 accacgtacg cgcccgcgag caccagccac gcgggcaccc acaggtacct cgcctcggag
      181 atgccgagcc cgcgcagcgc catcgtctga cgggtcagcg acatcccgcc gagccacgcc
      241 gtgatcgcgc tcgccgacgt cgccgcgaac aggatcgccg acagcggcgg cgcgagggcc
      301 agcacgtgca tcgagccgat cagcgagaac acccggtcgg gtcgcaccgg caggccgccc
      361 ccgccgagcg ccctgtgaaa gatgtacagc agcgtgcagc cgaccagcag cgacacaatt
      421 ataaagaacg gcgccggccg cagcagcgcc tgcgacagcg tcacgcgcgt gacctcggcg
      481 aggtcgcgtg gccgtcgcag cgccgccgga atttcggcga gcacccgcgc cgcgacggcg
      541 aacggcgcca gccagtctcg cacgagtcgg cgcagaccga cctgcgccgc ccgggagctc
      601 ccgtgcgagg ctgtctctcg ggacaggcgc tcgcgcaccg ccgtggcgag cgcctgctcg
      661 accttcgcga cctccgcggg tgagcgcgcc gcgggcacgt cgagctcgac cggtcggcgc
      721 agggcggggt ccatgtagat gaaccggtcg gcgagcgccg ccgcgaagcc gaggtcgtgc
      781 gtgacgacca gcgcgcccgc ctcgccctcg tcgatctgcc ggcgcagggt cgcggccagc
      841 agggtgatgc gatgcgggtc gaggccgacc gagggttcgt cgagcagcaa gatccgccgc
      901 ccgcccgcga gcgtgcgcgc gaccgccagg cgctgcgcct cgccgccgct gacctgcgcg
      961 accgcccgcc cgcgctcgcc ccacgcccgc ggcaggtcga cccgctcgat ccacgccgcg
     1021 accgcctcca cggtcgccgg cgccggcggc tcggcgttac gcagcgcgag ggcgatgttg
     1081 tcggcggcgc tcaaatggtc aaacagcgcc ccgcgctgcg gcacgatgcc gaccgccgtc
     1141 gccaccacgc tcggccccgt gcgcaccgcg aagccgcggt cgcgcaggct gtccgggtcg
     1201 tgcagcgcgg tcgtcagcgt cgacttgccg gccccgctcg ccccgaacac cgcgaccacc
     1261 tcgcgcgcgc gcagatcgat cgcgacgtcg acgagcagcg ggctgccgtc cggacggtcg
     1321 acgcacaacc cgcgcaccga cagcagcaac tcacctgtcc tttcagacag ctccgccgtc
     1381 tgctcctgtg cgggcgtcac acgccgagcc acgcgcgcag gcgggtcgcg tgcggctcgt
     1441 ggagctcgat gccgggctgg gtgacctcgg gcatcgcctc gccgtggaag tcggagccgc
     1501 cggtgacgac gaggtccatc tgcttcgcca tgcgcagcca cacctcgcgc tcggcgtggg
     1561 cgtagcggcc atacagcgcc tcgatgccgt cgaggccgtc ggcgcggtgg cggcggtaga
     1621 tgtcggcgac gagcgccgcg ctgcgcaggg tgtgcgggtg ggccagcgac atgcgggctc
     1681 cggcggcgcg cccgagggcg aggccctcgg cgagggtgat gcgtgcgaac gcgacgtcgg
     1741 ccgggccgtt gtcgtgaagg tagcgctcga aggcctctcg catgctgcgg accgccttca
     1801 ccgccaccag cgcgcgggcg atgtcggggc gtccgggggt gcggcctgcc gtctcacgaa
     1861 ggatctgctc gacgtcgagg tggatcccga gctcggcgag gcggccgacg atcgcccgca
     1921 ggcgcgcgcg acgatcgtcc tgcacgccca ccaggcgctc ctcgagcgcg tcggcccccg
     1981 gcccctcctt caccccgtag atcagcaggt ggatcgtgcg gctgtcgtgc ttgcagctca
     2041 gctcgacgcc acgcagcacc gtcacccccg gcagcgccgc ctgggtggcc gcgaagccgg
     2101 cgaggctgtc gtgatcggtg aggcagaaca gctcgacgcc ctggcgcccc gcccgtgtcg
     2161 ccacctcgtc ggcgctacag ctgccgtccg agtgggtgga gtggcagtgc aggtcgacac
     2221 gcatcccggg cagggtaccc gatctgcgcg cccccgcccc atccgcgacc aaaaactcac
     2281 acgagcgcgt aacgcaggcc ccggtcgcgc accaccagcg tgtcggcccc gcagtgctcg
     2341 agcgccgcca gcaagatcgt gacaccggcg acgatgacgt cggcacgccc gcgctcgagg
     2401 cccggccgct cgcagcgctg ggccagcgtc tcgctcgcca gcgcgtcgcg cagcgcctgc
     2461 acctgcgcga ccgtgaagcg gctgccgtcg accttgatgc ggtcgtacac cggcagcccc
     2521 agcagcagcg ccgccgtcgt cgtcgccgtg cccgcgagcg cgtgcaacac ggggtgcggc
     2581 gtcaccggca tcgacgcctg gaacgccgcc agcgcctccg cgcgcaccgc cgcgagcatc
     2641 tccgccgtgg gcgg
//
LOCUS       DASWNJ010000790         2362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_15053, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000790 DASWNJ010000000
VERSION     DASWNJ010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15053"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="VGB85_13285"
     CDS             <1..842
                     /locus_tag="VGB85_13285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR02587 family membrane protein"
                     /protein_id="HEY0135059.1"
                     /translation="RGIAGGLLFSLPLLYTMEMWEMGLVAEPSRLLVGLGFGLLLLLG
                     YNRFAGLRCDASLTEVVIDSVEELGLGLLLAATILWLLGSIDGDTRMPEVMGKVVIEG
                     LAVAIGVSVGTAQLGGESPGVVGDDSKNQYIEHRPVSLTQTLHGPSWFAAQLTLATCG
                     AVLIAANVAPTEEIEDIARGISVFQQLGLTGLTLAIGALVLYLSDFRGSRTHVLYHVR
                     MDVLFGALATYLVSMSAAAALLWFFGRFDGNGPAFCLAETIVLGLPTAIGASAGRLLL
                     QPQ"
     gene            839..1237
                     /locus_tag="VGB85_13290"
     CDS             839..1237
                     /locus_tag="VGB85_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135060.1"
                     /translation="MIKPPRKNWLEWTVFTAGLLLLLATTGYLGYQALVYGHRPAALA
                     VSLGDPWTPDPDHPSSVVVPVTVRNEGGHTAAEVTVEISLVRKGEPIERRELTFAFVP
                     HMSSRAGALTFEHRPDSNELQARVLGYLEP"
     gene            complement(1248..1706)
                     /locus_tag="VGB85_13295"
     CDS             complement(1248..1706)
                     /locus_tag="VGB85_13295"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY0135061.1"
                     /translation="MRDESIYDLLKADHKTVREIFEQMESTGGQVTRERLVGRLKLEL
                     LAHAEAEDTVFYKPLRLANEARDIILEAEEEHRVVTRLLGELERMSAEDEKWKARVTV
                     LKELVEHHVDEEEGEIFKKAKGIFDAELEAELGAAFLTEKRRLQAEMRAA"
     gene            complement(1768..2169)
                     /locus_tag="VGB85_13300"
     CDS             complement(1768..2169)
                     /locus_tag="VGB85_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135062.1"
                     /translation="MDNPYNSYRNNVQGASDFCAPDLIPYDLDFDAGVCAEKLDLSVY
                     VANQGCLGVGPGVNVSFYEETMGLLGTVQTTGTLVAGAAEKVTLAIPGKFESVTVWAV
                     ADDDGTGGGVLNECDEVNNSLPMSQLCIPPG"
BASE COUNT          327 a          816 c          791 g          428 t
ORIGIN      
        1 cgcgtggcat cgctggcggg ctgctgttta gcctgccgct gctctacacg atggagatgt
       61 gggagatggg cctggtcgcc gagccgtcgc gcctgctcgt cgggctcggc ttcggcttgt
      121 tgctgctgct cggttacaac cgcttcgccg gcctgcgctg cgacgccagc ctcaccgagg
      181 tcgtgatcga ctcggtggag gagctcggcc tcggtctgct gctcgccgcg acgatcctgt
      241 ggttgctcgg cagcatcgac ggcgacacgc gcatgccgga ggtgatgggc aaggtggtga
      301 tcgagggcct cgcggtggcg atcggtgtgt cggtcggcac ggcccagctc ggcggcgagt
      361 cacccggcgt cgtcggggac gacagcaaga accagtacat tgagcatcgc ccggtcagcc
      421 tgacccagac cctgcacggc ccgtcatggt tcgccgcgca gctgaccctg gcgacctgcg
      481 gcgcggtgct gatcgccgcc aacgtcgcgc cgaccgagga gatcgaagac atcgcgcgcg
      541 ggatctcggt gttccagcag ctcggcctga ccggcctcac cctggcgatc ggcgcgctgg
      601 tgctgtacct gagcgacttt cgcggctcgc ggacgcacgt gctgtatcac gtgcgcatgg
      661 acgtgctgtt cggcgccctc gcgacctacc tggtctcgat gtccgccgcc gcggcgctgc
      721 tgtggttctt cggccgcttc gacggcaacg gtccggcgtt ttgcctcgcc gagacgatcg
      781 tgctcggcct gcccactgcg atcggcgcct ccgccggacg cttgctcttg caaccacaat
      841 gatcaaaccc ccgcgcaaga actggctcga gtggaccgtc ttcaccgccg gcctgctgct
      901 gttgctcgcc accaccgggt acctcggcta ccaggcgctc gtgtacgggc accggcccgc
      961 ggcgctcgcg gtcagcctcg gcgacccgtg gacccccgac cccgaccacc cctcgagcgt
     1021 cgtcgtgccg gtcacggtgc gcaacgaggg cggccacacg gccgccgagg tcacggtcga
     1081 gatctcgctg gtacgaaagg gcgagccgat cgagcgccgc gagctgacct ttgcgttcgt
     1141 gccgcacatg tcgtcacgcg ccggcgccct caccttcgag catcggccgg acagcaacga
     1201 gctacaggcg cgggtgctcg ggtacctcga gccatgagat aggcagctca ggcggcgcgc
     1261 atctcggcct gcaggcgccg cttctcggtg aggaaggcgg cgccgagctc ggcctcgagc
     1321 tccgcgtcga agatcccctt cgccttcttg aaaatttcgc cctcctcctc gtcgacgtgg
     1381 tgctcgacga gctccttgag caccgtcacg cgggccttcc acttctcgtc ctcggcgctc
     1441 atgcgctcga gctcgccgag caggcgggtg acgacgcggt gctcctcctc cgcctcgagg
     1501 atgatgtccc gcgcctcgtt ggcgagccgc agcggcttgt agaagacggt gtcctcggcc
     1561 tcggcgtggg cgagcagctc cagcttgagg cggccgacca ggcgctcgcg cgtcacctgg
     1621 ccgccggtgc tctccatctg ctcgaagatc tcgcggaccg tcttgtggtc cgccttgagg
     1681 aggtcgtaga tgctttcgtc gcgcatgagc ttgctatggc gcggactcgc ccgactggca
     1741 acctgccata gctttcatcg cgccacttca gcccggcggg atgcacagct gcgacatcgg
     1801 caggctgttg ttgacctcgt cgcactcgtt gagcactccg ccgcccgtgc cgtcgtcgtc
     1861 ggcgaccgcc cacaccgtca ccgactcgaa cttgccgggg atcgcgagcg tcaccttctc
     1921 ggccgcgccg gcgaccagcg tgccggtcgt ctgcaccgtg ccgagcaggc ccatcgtctc
     1981 ctcgtagaat gagacgttga cgcccggacc gacgccgagg cagccctggt tggcgacgta
     2041 gaccgagagg tcgagcttct cggcgcacac gcccgcgtcg aagtcgaggt cgtacgggat
     2101 caggtccggc gcgcagaagt cggaggcgcc ctggacgttg ttgcgatagc tgttgtacgg
     2161 gttgtccatc ggcgtggtcc agctgttgtc ctccttgacg gggatctcgc cgacgaggcc
     2221 gacattggta atgtggtacg tgtggtcgct taaggggccc tacgagcccg aattcgaggg
     2281 tttgtctcga gacagacccg aatcgcgaca aaatcgggac aggatcgggc cgagacccgg
     2341 ggatctcgcc gccgcgcatg tg
//
LOCUS       DASWNJ010000791         1616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_119930, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000791 DASWNJ010000000
VERSION     DASWNJ010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119930"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..129
                     /locus_tag="VGB85_13305"
     CDS             <1..129
                     /locus_tag="VGB85_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEY0135063.1"
                     /translation="RGGRVKDLPGVRYKIIRGSLDTQGVRNRKQARSRYGAKKEKS"
     gene            129..599
                     /gene="rpsG"
                     /locus_tag="VGB85_13310"
     CDS             129..599
                     /gene="rpsG"
                     /locus_tag="VGB85_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEY0135064.1"
                     /translation="MPRKGPAPKHPVVTDPVYGSPLVTSLINKVLIGGKRSVAERIVY
                     GALEGAREKSGADPVITLKRALDNVKPTLEVRSRRVGGATYQVPIEVRAGRSNTLALR
                     WLIQYSRARREKTMTERLMNELLDASNGLGAAVKRREDTHKMAESNKAFAHYRW"
     gene            689..>1616
                     /locus_tag="VGB85_13315"
     CDS             689..>1616
                     /locus_tag="VGB85_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0135065.1"
                     /translation="MASNAILAKTRNIGIMAHIDAGKTTTTERILYYTGRAYKIGEVH
                     DGAATMDWMEQEQERGITITSAATTCQWADHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VAVFDSVAGVEPQSETVWRQADKYSVPRICFVNKMDRTGANFYRTVDMIVSRLHATPL
                     VVQLPWGTEADFTGVIDLVQMKGLLWANDDKTSKGDHYDVVDIPAEYAEQAAEYRTKL
                     VETLADVDDAVMEKYLEGEEISNDEIKAGVRRAILSSTKERTVTAVLCGSAFKNKGVQ
                     PMLDAVVDSLPSPLDVGAVTGHSVKDETVLEQR"
BASE COUNT          303 a          596 c          515 g          202 t
ORIGIN      
        1 cgcggcggcc gcgtgaagga cctccccggc gtccgctaca agatcatccg tggctcgctc
       61 gacacccagg gcgtccgcaa ccgcaagcag gcgcgcagcc gctacggcgc gaagaaggag
      121 aagagctagt gcctcgcaag ggtcctgccc cgaagcaccc ggtcgtcacc gacccggtct
      181 acggctcgcc gctcgtcacg agcctcatca acaaggtcct catcggcggc aagcgctccg
      241 tcgccgagcg gatcgtctac ggcgcgctcg agggcgcccg cgagaagtcc ggcgccgacc
      301 cggtcatcac gctcaagcgc gcgctcgaca acgtgaagcc gaccctcgag gtccgcagcc
      361 gccgcgtcgg tggtgccacc taccaggtgc cgatcgaggt ccgcgccggc cgcagcaaca
      421 cgctcgcgct gcgctggctc atccagtact cccgcgcccg tcgcgagaag acgatgaccg
      481 agcggctcat gaacgagctg ctcgacgcca gcaacggcct cggtgccgcg gtcaagcgcc
      541 gcgaggacac ccacaagatg gccgagtcga acaaggcctt cgcgcactac cgctggtagt
      601 cgacgcaccc gcacgcaccg ccttcggcgg caggcccacc ccgccgaccg ccgtacgcac
      661 ccgagacccc ggaagaagag aagcagctgt ggccagcaac gcgatcctcg ccaagacccg
      721 caacatcggg atcatggcgc acatcgacgc gggcaagacg acgacgaccg agcggatcct
      781 gtactacacc ggtcgggcgt acaagatcgg cgaggtccac gacggcgccg ccacgatgga
      841 ctggatggag caggagcagg agcgcggcat caccatcacg tccgccgcga cgacctgcca
      901 gtgggccgac caccgcatca acatcatcga caccccgggc cacgtcgact tcaccgtcga
      961 ggtcgagcgc tcgctgcgcg tgctcgacgg cgccgtcgcg gtcttcgact cggtcgccgg
     1021 cgtcgagccg cagtccgaga cggtgtggcg gcaggccgac aagtactcgg tcccgcgcat
     1081 ctgcttcgtc aacaagatgg accgcacggg cgcgaacttc taccgcaccg tcgacatgat
     1141 cgtgtcccgg ctgcacgcca ccccgctggt cgtccagctg ccgtggggca ccgaggccga
     1201 cttcaccggt gtcatcgacc tggtgcagat gaagggcctg ctctgggcca acgacgacaa
     1261 gacgagcaag ggcgaccact acgacgtggt cgacatcccg gccgagtacg ccgagcaggc
     1321 tgcggagtac cgcaccaagc tcgtcgagac gctcgccgac gtcgacgacg ccgtcatgga
     1381 gaagtacctc gagggcgagg agatctccaa cgacgagatc aaggccggcg tccgccgggc
     1441 catcctgtcc tcgaccaagg agcgcaccgt cacggccgtg ctctgcggct cggcgttcaa
     1501 gaacaagggc gtccagccca tgctcgacgc cgtcgtcgac tccctgccct cgccgctcga
     1561 cgtcggcgcc gtgaccggcc actcggtcaa ggacgagacg gtcctcgagc agcgtg
//
LOCUS       DASWNJ010000792         3331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_119932, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000792 DASWNJ010000000
VERSION     DASWNJ010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119932"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1951
                     /locus_tag="VGB85_13320"
     CDS             170..1951
                     /locus_tag="VGB85_13320"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0135066.1"
                     /translation="MNATLDVRVLLHGEAVDNLRFTQPVIRIGRERDAQGAVNDIVLT
                     SKGVSRSHAQILVGDDGVTVIDRSRHGTFINGERLHGPRRLEARDVVEIDAYTIQCEL
                     AAGTPVTAPAVTPVNRPVTSPPLPDTTAAELNLVPLDDDLPSFDLGDLGDLRDSTPPR
                     VETLPVVAPRVADAPAVPRLPELPGPESLHAAPHAAPVTIAAVAPAVAPTSAPETSPL
                     ALAYRELAAQHGASRWGQPPALAHGDLPRATEAARRLLGSRMQALPAGVPWPERLARE
                     LCGLGPLDDLLDDVSVTALSVRGTAAIDVRRGDAREPAAARFSCVEAIHAMLERWTGQ
                     RLGERGQLEAGPVEGTSVLALDRNLAPGGPIVHVTRARRPGHTVEGSPAALAGLVGER
                     MLPQAAADLLAECVRRGRTLLLHGDASADLSTLTRALIAARPTDRSAAIVRRSGAWDP
                     RELFILAGERPGALAAARRLDPDWLVVEEIEATDAGELCGGVRHPGGGTIGTLRARSA
                     EAALARLTALFAAANGWSDLQAARAVVTTGFDVFVGLRRASDGRSVVHTISEARSGAR
                     AELAELFTWNADIAGILPTDVEIHLLR"
     gene            1960..2712
                     /gene="tssJ"
                     /locus_tag="VGB85_13325"
     CDS             1960..2712
                     /gene="tssJ"
                     /locus_tag="VGB85_13325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HEY0135067.1"
                     /translation="MLRHLLLVPVLIAATSACKSRGECTADPVGVQFLAVSHPVLNTD
                     DAGKSWPTNLRIYELKKDIDLEQLEFDTVLHESDKAFGDALVKSHEQVIYPDRRKPWQ
                     LVLDPSTRHVVTVGLFREPIADAWYQAYTLPADHAAQRCEAKRRGKTPADPCVFLAFE
                     RNEVSGGRFPPAGFDVKAFSATCAPVIAAPSKQPKKNKKRRSWRIPELPQTPRAPQTP
                     QAPQAPQAPQGPQMPQAPRGPTAPQAPRLPGR"
     gene            2714..>3331
                     /gene="tssK"
                     /locus_tag="VGB85_13330"
     CDS             2714..>3331
                     /gene="tssK"
                     /locus_tag="VGB85_13330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HEY0135068.1"
                     /translation="MTIATASRPLWSEGMLMCPQHMQQQDLYHEQHVHARLQALSATP
                     WGVTSVQFDTSALKAGTVQLNGFRGVLPDGTPAHFDALSPLRPPSRAVGPHFPAKQES
                     LGVYLGLPLMREGVANYALSSDASAAQRFRGVVRRVFDLTLARNERELQVSEPALVLL
                     FDGEQHKDHAILKIGEIRHDQAGGYALAESFIAPALAISAAPHLVA"
BASE COUNT          482 a         1322 c         1062 g          465 t
ORIGIN      
        1 ttctgccggc gggtgcaacc cgccgccgcc gccagagtcg tccacatgac catccacagc
       61 gccgtacgtc gcgtcatctc cgggaggtcg taaccgcagc gcgaagcgcc gtcaaaccca
      121 actggacttg catattgcaa ggtttacgat tccgcggttt gttgtcatca tgaacgcgac
      181 gctcgatgtc cgcgtgctcc tccacgggga ggcggtcgac aatcttcgct tcacgcagcc
      241 ggtgatccgc atcggtcgcg agcgcgacgc ccagggcgcg gtcaacgaca tcgtactgac
      301 cagcaagggc gtctcgcgca gtcacgccca gatcctcgtc ggcgacgacg gcgtgaccgt
      361 catcgaccgc tcgcgccacg gcaccttcat caatggcgag cgactccacg gcccgcggcg
      421 cctcgaggcc cgcgacgtcg tcgagatcga tgcatacacg atccagtgcg agctcgcggc
      481 gggtacgccc gtgaccgcgc cggctgtcac gcccgtgaac aggcccgtga ccagcccacc
      541 gctgcccgat acgaccgccg ccgagctcaa cctcgtgccg ctcgacgacg acctgccgag
      601 cttcgacctc ggcgacctcg gcgacctccg tgactcgacc ccccctcggg tcgagacgct
      661 ccccgtcgtc gcgccacgcg tcgccgatgc tcccgcggtc ccacgactcc ccgagcttcc
      721 aggtcccgag tcccttcacg cagcacctca cgcagcaccg gtgaccatcg ccgcggtcgc
      781 gcccgcggtc gcgcccacca gcgcaccgga gacctcgccg ctcgcgctcg cctaccgtga
      841 gctcgccgcc cagcacggcg cctcgcgctg gggtcagccg ccagcgctgg cgcacggcga
      901 cctcccgcgg gcgaccgagg cggcgcgcag gctcctgggc tcgcgcatgc aagcgctgcc
      961 cgccggcgtc ccctggcccg agcgactcgc ccgcgagctg tgcgggctcg gtccgctgga
     1021 cgacctgctg gacgacgtca gtgtgaccgc gctcagcgta cgcggcaccg cggcgatcga
     1081 cgtccgtcgc ggtgacgcgc gcgagcccgc ggcggcccgt ttctcgtgtg tcgaggccat
     1141 ccacgcgatg ctcgagcgct ggaccggcca gcgcctcggc gagcgtgggc agctcgaggc
     1201 cgggccggtc gagggcacgt cggtcctcgc gctcgaccgc aacctcgccc ctggcggacc
     1261 gatcgtccac gtcacccgcg cgcgccggcc cggacacacg gtcgagggca gcccagccgc
     1321 gctcgccggg ctggtgggcg agcgtatgct cccgcaagcc gcggccgacc tgctggccga
     1381 gtgcgtccgt cgcggtcgca cgcttctcct gcacggcgac gcctcggccg atctgtcgac
     1441 gctgacgcgt gccctgatcg cggcgcgtcc gaccgaccgc agcgcggcga tcgtccgtcg
     1501 aagcggcgca tgggatcccc gcgagctgtt catcctcgcg ggcgagcgcc cgggagccct
     1561 cgccgcagcg agacgcctcg accccgactg gctcgtcgtc gaagagatcg aggccaccga
     1621 cgccggtgag ctctgcggcg gcgtgcgtca ccctggtggc ggcacgatcg gcacgctgcg
     1681 cgcccgctcg gcggaggccg cgctggctcg cctcacggcc ctgttcgcgg ccgcgaacgg
     1741 atggtccgac ctccaggctg cccgcgccgt cgtcaccacc ggcttcgacg tgttcgtcgg
     1801 cctccgccgc gcatccgacg gcagatcggt ggtccatacg atctccgagg cgcgctccgg
     1861 tgcgcgcgcc gagctcgctg aactcttcac ctggaacgcc gacatcgccg ggatcctccc
     1921 gacggatgtc gagatccacc tgctgcgctg aggtcccccg tgctgcgtca cctgctcctc
     1981 gttccggtcc tgatcgctgc cacgtcggcc tgcaagtccc gcggtgaatg caccgccgac
     2041 cccgtcggcg tgcaatttct cgccgtctcg caccccgtcc tcaacacgga cgacgccggc
     2101 aagtcatggc cgaccaacct tcgcatctac gagctcaaaa aggacatcga cctcgagcag
     2161 ctcgagttcg acacggtcct gcacgagtcc gacaaggcgt tcggcgacgc cctcgtcaag
     2221 tcgcacgagc aggtcatcta ccccgaccgg cgcaagccct ggcagctggt cctcgacccg
     2281 agcaccaggc acgtcgtcac cgtcgggctg ttccgcgagc cgatcgccga cgcgtggtac
     2341 caggcctaca cgctgcccgc ggaccacgcc gcgcagcgct gtgaggccaa acgccgcggc
     2401 aagacgccgg ccgatccctg cgttttcctc gccttcgagc gcaacgaggt ctcgggcggc
     2461 cgcttcccgc ccgccggctt cgacgtcaag gcgttctcgg cgacctgcgc ccccgtgatc
     2521 gcggcgccga gcaagcagcc caagaaaaac aagaagcgcc gatcctggcg tatccccgag
     2581 ctaccgcaga ccccgcgggc tccgcaaacc ccacaggcac cgcaggcgcc gcaagccccg
     2641 cagggcccac agatgccgca ggcgccgcgc ggcccgaccg cgccccaggc tccgcgctta
     2701 cccgggagat gacatgacga tcgccactgc atcacgaccg ttgtggagcg agggcatgtt
     2761 gatgtgcccc cagcacatgc aacagcaaga tctctaccac gagcagcacg tgcacgcgcg
     2821 gctgcaggcg ctgtccgcca cgccctgggg ggtgacgagc gtgcagttcg acacctccgc
     2881 gctcaaggcc ggcaccgtgc agctcaacgg ctttcgcggc gtgctgcccg acggcacgcc
     2941 ggcccacttc gacgcgctca gcccgctgcg cccgccgagc cgcgccgtcg gcccccactt
     3001 tccggccaag caggagtcgc tcggcgtcta cctcggtctg ccgttgatgc gcgagggcgt
     3061 cgccaactac gcgctgtcga gcgacgcctc ggccgcccag cggtttcgcg gcgtcgtgcg
     3121 ccgggtcttc gacctcaccc tcgcccgcaa cgagcgcgag ctgcaggtca gcgagccggc
     3181 gctggtgctc ctgttcgacg gcgagcagca caaggaccac gcgatcctca agatcggcga
     3241 gatccggcac gaccaggccg gcggctacgc cctcgcggag agcttcatcg cgccggcgct
     3301 cgcgatctcg gcggcccccc acctcgtcgc c
//
LOCUS       DASWNJ010000793         2951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_440184, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000793 DASWNJ010000000
VERSION     DASWNJ010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440184"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VGB85_13335"
     CDS             <1..590
                     /locus_tag="VGB85_13335"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HEY0135069.1"
                     /translation="FPAGIFQPPYFDATWPSAINFGGVGLAMGHELTHAFDTWGSRRD
                     ARAQARNWWSAATADAFADKAKCIAAHLSTYEAAPGIPMEASRVLDEAIADIGGLNIA
                     HRAYRQLAAKHGEDPSARFSRRLTHEQLFFVASAQNFCVKQTPEFEQAQARSDNHALG
                     RVRVNATAAQSPAFCGAFTCEPGEPMHARIECDLW"
     gene            1140..1213
                     /locus_tag="VGB85_13340"
     tRNA            1140..1213
                     /locus_tag="VGB85_13340"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1174..1176,aa:Pro,seq:tgg)
     gene            1220..1292
                     /locus_tag="VGB85_13345"
     tRNA            1220..1292
                     /locus_tag="VGB85_13345"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1253..1255,aa:Arg,seq:acg)
     gene            1296..1370
                     /locus_tag="VGB85_13350"
     tRNA            1296..1370
                     /locus_tag="VGB85_13350"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1331..1333,aa:Pro,seq:cgg)
     gene            1432..1506
                     /locus_tag="VGB85_13355"
     tRNA            1432..1506
                     /locus_tag="VGB85_13355"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1467..1469,aa:Pro,seq:ggg)
     gene            1520..1603
                     /locus_tag="VGB85_13360"
     tRNA            1520..1603
                     /locus_tag="VGB85_13360"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1554..1556,aa:Leu,seq:gag)
     gene            1641..1724
                     /locus_tag="VGB85_13365"
     tRNA            1641..1724
                     /locus_tag="VGB85_13365"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1675..1677,aa:Leu,seq:cag)
     gene            1806..>2951
                     /locus_tag="VGB85_13370"
     CDS             1806..>2951
                     /locus_tag="VGB85_13370"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0135070.1"
                     /translation="MTDGLAPGTLLGGRYRIVRLLAEGGMGVVYEGEHLDLGRRVAIK
                     TLLSELQDQPRLIQRFIREARATCMISHPNVVSVLDIDQTPDGLVFFVMEYLEGEDVS
                     QVLEREGAIAWDRARRIALQVCEALGAAHDRGVVHRDLKPSNCFLVRDENGDERVKVL
                     DFGIAKVLESSAPRAGSRGPGTGRDLRWLRTSTGEVFGTLAYMAPEHLNGDLHDHHVD
                     IYAIGVILHEMLTGAPPFSPDVPGTFIQQVLFEPPTPPSQLAPHAGIPPDLDYVVMRA
                     LEKRPNDRFASMDELAATLTSVRSEAAPAIPVPWIATPVLAPVAVKPVPAAPARPRAA
                     PTRRSWVPGVAGLLGVGAVLVATVLAMLVMRTPPGTTQELATPPEAPA"
BASE COUNT          467 a         1026 c          996 g          462 t
ORIGIN      
        1 agttccccgc cgggatcttc cagccgcctt atttcgatgc gacgtggccg agcgcgatca
       61 actttggtgg cgtggggctg gcgatgggcc acgagctgac ccatgccttc gatacgtggg
      121 gcagtcggcg cgacgcgcga gcccaggccc gcaactggtg gagcgccgcg acggcggacg
      181 cgttcgccga caaggccaag tgcatcgccg cgcatctatc cacctacgag gccgccccgg
      241 gcatcccgat ggaggcgagc cgcgtcctcg acgaggcgat cgccgacatc gggggcttga
      301 acatcgccca ccgggcgtat cgccagctcg cggcgaagca cggcgaggat ccctcggccc
      361 ggtttagccg gcgtctcacc cacgagcagc tgttcttcgt ggcgtccgcc cagaacttct
      421 gcgtgaagca gaccccggag ttcgagcaag cccaggcgcg cagcgacaac cacgccctgg
      481 gccgcgttcg cgtcaacgcg actgccgctc agtcccccgc attttgcggc gcgttcacct
      541 gcgaaccggg cgagcccatg cacgccagga tcgagtgcga tctgtggtga gtgcacgccg
      601 cgggcgatgg cgacgccctg ctcgcgcagg tggacgggcc catgccgcgc cgggcgcgcg
      661 cccggcactc tgcggccgcg accgaccgcg ctcggaatca acacttcacg tccggcccgg
      721 ggaccaatgt cggtacttga aaaaaatcta cgatgaatgc ttgacgagcg tgccgccgcg
      781 gcgtagatag tgatcctcga aggcaacagt atgacgacgg caagcaacat ccatgggtcg
      841 acgctccagt tccagctccg cgctggtctg ccgctccccg tgcgtccgct tgctggcctg
      901 ctgccagcgc tacgccgccc gctgccgcgc cgcctcccca ggcggtgaac gccccggtcg
      961 gggcgtaggc acgcgctggt ggctgcgcgc agcaaaattg ccacgatgac gccccgtcgg
     1021 ggacgtcgtc cgcgagacgc ccgcacaggc gcagcgcccc cgtctgacac acggccgctg
     1081 acgccgaggt acgcacattg tgccgtgccg cggaaggccc gcgcaactga ataacacgtc
     1141 gaggtgtggc gcaacctggt agcgcgtctg gtttggaacc agggggatgc aggttcaaat
     1201 cctgccacct cgacacccac ggcacgtagc tcaatggata gagcaccaca ctacgaatgt
     1261 ggtggttgga ggttcgagcc ctcccgtgcc gactccggga tgtagcccag tctggtcagg
     1321 gcgcctgctt cggatgcagg aggtcgcagg ttcgaatcct gccatcccga ttctcgcgtt
     1381 ccttctcaag gaggagaaga aagacgaccc cattttgaag agacgtccat tcgggatgta
     1441 gctcagccag gccagagcgc gtgctcgggg tgcacgaggg cgcaggttcg aatcctgtca
     1501 tcccgtcttc attgttcgag cgcaggtggt ggaattggta gacacgcggc cttgaggtgg
     1561 ccgtgcccgc aagggcgtga gggttcgagt ccctccctgc gcatctcgca ttatctttat
     1621 aaagatcgac atcgaccgcc gcgcaggtgg tggaactggt agacacgcgg gcctcagaag
     1681 cccgtgcccg cgagggcgtg agggttcgag tccctccctg cgcattgctg ttctggatgg
     1741 gccggcgacg ttcgttccag gcccgaactt gcgcccgggg tgcgggtctg cgacccttcg
     1801 cgggcatgac cgatggcctc gcaccgggca cgctcctcgg gggtcgttac cggatcgttc
     1861 ggctgctcgc cgagggtggc atgggcgtcg tgtacgaggg cgaacacctc gacctcggcc
     1921 gacgcgtggc gatcaagacg ctgctgtcgg agctgcaaga tcagccgcgg ctgatccagc
     1981 gctttatccg ggaggcgcgc gccacatgca tgatctccca cccgaacgtc gtcagcgtcc
     2041 tcgacatcga ccagaccccc gacgggctgg tgttcttcgt gatggagtac ctcgagggcg
     2101 aggacgtgtc gcaggtgctc gagcgcgagg gggcgatcgc gtgggaccgg gcccggcgga
     2161 tcgcgctgca ggtgtgcgag gccctcggtg cagcgcacga ccggggggtg gtgcatcgcg
     2221 acctcaagcc ctccaactgc ttcctggtgc gcgacgagaa cggcgacgag cgcgtcaagg
     2281 tgctcgactt cgggatcgcc aaggtgctcg agtccagcgc gccacgggcc gggtcccgcg
     2341 gacccggaac cggccgcgac ctccgctggc tccgcaccag caccggcgag gtgttcggga
     2401 cgctcgcgta catggccccc gagcacctca acggcgacct gcacgaccac cacgtcgaca
     2461 tctacgcgat cggcgtgatc ctccacgaga tgctcacggg cgcgcctccg ttctcgccgg
     2521 atgtgccggg caccttcatc cagcaggtgc tgttcgaacc gccgacgccg ccgagccagc
     2581 tcgcgcccca cgccggcatc cccccggacc tcgactacgt cgtgatgcgc gcgctggaga
     2641 agcgcccgaa cgaccgcttc gccagcatgg acgagctcgc ggcgaccctg acgtccgtgc
     2701 gtagcgaggc cgccccggcg atccccgtgc cgtggatcgc aaccccggtc ctcgcacccg
     2761 tggcggtcaa acccgtcccc gccgcgccgg ctcgcccgcg cgcggcgccg acacgacgaa
     2821 gctgggtgcc aggggtcgcg ggcctgctcg gcgtcggggc ggtgctggtg gcgaccgtgc
     2881 tcgccatgct ggtcatgcgg acgcctcccg ggacgacgca ggagctcgcc acgccgcccg
     2941 aggcacctgc g
//
LOCUS       DASWNJ010000794         1714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_226273, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000794 DASWNJ010000000
VERSION     DASWNJ010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226273"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VGB85_13375"
     CDS             complement(<1..765)
                     /locus_tag="VGB85_13375"
                     /inference="COORDINATES: protein motif:HMM:NF039196.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEY0135071.1"
                     /translation="MPSATTTPRRSSGGESPLDTKLLLKTLMAMRRGDFSVRLPIDQT
                     GVAGKIYDTLNEIVDLNQRMSDEFERVSVVVGKDGKITQRAVLGGAVGAWADTIGSVN
                     SLIADLVQPTAEVGRVIGAVAKGDLSHSMAEEIEGRPLDGEFLRTARIVNTMVDQLRS
                     FASEVTRVAREVGTEGKLGGQAEVKGVSGTWKDLTDSVNSMASNLTGQVRNIAEVTKA
                     VANGDLSKKITVDVKGEILELKNTINTMVDQLNSFAY"
     gene            complement(809..>1714)
                     /locus_tag="VGB85_13380"
     CDS             complement(809..>1714)
                     /locus_tag="VGB85_13380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0135072.1"
                     /translation="ITVRDNGIGIDSELLPNVFEMFEQGKQSLDRSQGGLGVGLTLVK
                     RLVELHGGTVVARSAGEGTGSEFVVHLPGVGRQAHAPSLNGVDAPRRPSAWIPGQEEA
                     QEAAEEAAEAAAAAVAAASSASSASSASTASGTAATGILADPTEADRTLIHMPLPVLP
                     VPPLDTPRSQSEPAPAAVEDTPRRLRILVVDDNPDIRATLHDLLEMQGHHVDVAEDGP
                     QAIERVMALRPQVALVDIGLPGMDGCEVARTLRATPGVTTRLVAMTGYGQPEDRKRAL
                     DAGFDAHVVKPVELDELTRLLYEHS"
BASE COUNT          234 a          582 c          610 g          288 t
ORIGIN      
        1 gtaggcgaac gagttgagct ggtcgaccat cgtgttgatc gtgttcttga gctcgaggat
       61 ctcgcccttg acgtcgaccg tgatcttctt cgaaagatcg ccgttggcga ccgccttggt
      121 cacctcggcg atgttgcgca cctggccggt caggttgctc gccatcgagt tgacggagtc
      181 ggtcaggtcc ttccaggtgc ccgacacgcc cttgacctcg gcctggccgc cgagcttgcc
      241 ctcggtgccg acctcacggg cgacgcgcgt cacctcggag gcgaacgacc gcagctggtc
      301 gaccatcgtg ttgacgatcc gcgcggtgcg caggaactcg ccgtcgagcg gccggccctc
      361 gatctcctcg gccatcgagt gcgacaggtc gcccttggcg accgcaccga tcacgcgacc
      421 gacctcggca gtgggttgca cgaggtcggc gatcagcgag ttgaccgagc cgatcgtgtc
      481 cgcccacgca ccgacggcgc cgccgagcac ggcgcgctgc gtgatcttgc cgtccttgcc
      541 gacgacgacg ctgacgcgct cgaactcgtc cgacatgcgc tggttgagat cgacgatctc
      601 gttgagcgtg tcgtagatct tgccggcgac gccggtctga tcgatcggca aacgcaccga
      661 aaagtccccg cgacgcatcg ccatcagggt cttgagcagc aacttcgtgt ccaggggact
      721 ctccccgccg ctgctgcggc gaggcgtcgt ggtggcgctc ggcatgtcgg tctcctcgaa
      781 gggttctcga aaactcgcta actaaggctc aggagtgctc gtagagcagg cgggtcagct
      841 cgtcgagctc gacgggcttg accacgtggg cgtcgaagcc ggcgtcgagg gcccgcttgc
      901 ggtcctccgg ctggccatag ccggtcatcg ccaccaggcg cgtggtcacg cccggcgtcg
      961 cgcgcagcgt gcgggcgacc tcgcagccgt ccatgccggg caggccgatg tcgacgagcg
     1021 cgacctgggg tcgcagcgcc atcacgcgct cgatcgcctg cggaccgtcc tcggcgacgt
     1081 cgacgtggtg cccctgcatc tcgagcaggt cgtgcagggt cgcgcgaatg tcggggttgt
     1141 cgtcgacgac aaggatccgc aggcgtcgcg gcgtgtcctc gaccgccgcg ggtgccggct
     1201 ccgactgcga gcgcggcgtg tcgagcggag gcaccggcag gaccggcagc ggcatgtgga
     1261 tgagggtcct gtcggcctcg gtcgggtcgg ccaaaatccc tgtggccgct gtccccgatg
     1321 cagtggatgc ggaggatgcg gaggatgcgg aggatgcagc ggccacagcg gccgcggcgg
     1381 cctcggcggc ctcctcggcg gcctcctggg cctcctcctg accggggatc caggcgctcg
     1441 ggcgcctcgg cgcgtcgacg ccgttgaggc tcggcgcgtg ggcctggcgg ccgacgccgg
     1501 gcaggtgcac gacgaactcg ctgcctgtgc cctcgccggc gctgcgggcg acgacggtgc
     1561 ccccgtgcag ctcgacgagg cgcttgacga gcgtgaggcc gacgccgagg ccgccctggc
     1621 tgcggtcgag cgactgcttg ccctgctcga acatctcgaa cacgttcggc aggagctcgc
     1681 tgtcgatgcc gatgccgttg tcgcgcaccg tgat
//
LOCUS       DASWNJ010000795         4127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_579935, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000795 DASWNJ010000000
VERSION     DASWNJ010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579935"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VGB85_13385"
     CDS             complement(<1..923)
                     /locus_tag="VGB85_13385"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HEY0135073.1"
                     /translation="MTAPIPHNSSGKKVYFVSLGCPKNQVDTEIMLGVVRDQGHELVD
                     SAEDADTLVVNTCGFIEDAKQESIDTILELAQAKAAGKGKRLVVAGCLSQRYPEELAK
                     EMPEVDNFLGSADMLGLAQVLQGGAARMGVSPLGRRAYLYDHTTPRQVSGKRHSAYLK
                     IAEGCDRPCAFCIIPKLRGAQRSRTIASIVAEAEALVSNGARELCLVAQDLTTYGTDL
                     DDKPSFELLLDGLCQVRDLRWIRLHYAFPTTVTDGLLKQISSEPKIATYLDVPLQHVD
                     TEVLKRMRRGYTEKVVRNLVDRLRAQSNYIHL"
     gene            complement(997..2709)
                     /locus_tag="VGB85_13390"
     CDS             complement(997..2709)
                     /locus_tag="VGB85_13390"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEY0135074.1"
                     /translation="MGLCASATLACATCGAPDATNVATGPATTAEPTPAAAPRLTVDI
                     FAFARVLGTIAPCGCTTEPLGGLQYAFGYIEANSRPQTRLVLEPGSFLFADPAGPEAA
                     KDEPAWAQAEQRAAALQTRFAGLGAQLVSGLGPTDLSSPKADLSLETYPLPRVLANSR
                     KLDARGVQRHRLVRLGDDALAIDAGVTAVVEPGLAPSLAATAPAEALRGEIKAMREAG
                     AELTVVLLQGSRATAEQLAREVEGIDLIVVGIPVGLERTRLGGPPARLGNTWVVEPGE
                     QAQTLTHLRLEIDPAVAAKLPAVDSWTIVPTVETQRRELARLDARLEKFRADVSADKA
                     FVARLEQERAALAASIEKPAPVSGAVVVTVAQTKVSCHLAVDPDAAKALHSYDNWVAA
                     KNQQRFAGVRAPAPTKGQAGYVGSEQCEACHADEAAHWAKTRHGDAYATLVKANKQFD
                     LSCVGCHVTGFREPGGSEVVENAGLQNVQCEQCHGPGSAHVAMPEKRGKANAIRRDAS
                     VEVCQSCHTPEHSDTFEYAAYLRDVLSPEHGPERRAALGEGPTGRELRAAGLAAAGGG
                     CKKM"
     gene            3093..3359
                     /gene="rpsP"
                     /locus_tag="VGB85_13395"
     CDS             3093..3359
                     /gene="rpsP"
                     /locus_tag="VGB85_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEY0135075.1"
                     /translation="MAVKIRLARMGSKKRPYYRIVAADARSPRDGRFLERIGTYDPHA
                     ETVVVNEARVSYWQGVGAHPTITVARLLRKARKAAKGTTPAAKA"
     gene            3650..3904
                     /locus_tag="VGB85_13400"
     CDS             3650..3904
                     /locus_tag="VGB85_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEY0135076.1"
                     /translation="MSSSPNISLKELIEYLARALVDHPNEVSVSEVAGEQTIVLELKV
                     AKDDLGKVIGKQGRTVKAMRALLSAASAKLRKRADLEILE"
     gene            3908..>4127
                     /locus_tag="VGB85_13405"
     CDS             3908..>4127
                     /locus_tag="VGB85_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135077.1"
                     /translation="MSAFKPDARTPVVGSATRQTSGAPSPETLAVAVVVGAHGIRGWV
                     RVHLHDPKSRALRPGLRVTLHGPEGERPS"
BASE COUNT          571 a         1481 c         1453 g          622 t
ORIGIN      
        1 aggtggatgt agttcgactg ggcgcgcagg cggtcgacga ggttgcggac caccttctcc
       61 gtgtagcccc ggcgcatgcg cttgagcacc tcggtgtcga cgtgctgcag cggcacgtcg
      121 aggtacgtgg cgatcttcgg ctcgctcgag atctgcttga gcagcccatc ggtgacggtg
      181 gtcgggaacg cgtagtgcag acggatccac cgcaggtcgc ggacctggca cagcccatcg
      241 agcagcagct cgaagctcgg cttgtcgtcg aggtcggtgc cgtaggtcgt caggtcctgg
      301 gcgacgaggc acagctcgcg cgcgccgttg ctgaccagcg cctcggcctc cgcgacgatg
      361 ctggcgatcg tgcgactgcg ctgcgcgccg cgcagcttgg ggatgatgca gaacgcgcac
      421 ggccggtcgc agccctcggc gatcttcagg tacgcgctgt ggcgcttgcc gctgacctgg
      481 cgcggggtcg tgtggtcgta gaggtaggcg cggcggccga gcggcgacac gcccatgcgc
      541 gcagcgccgc cctgcagcac ctgcgcgagc cccagcatgt ccgcggagcc gaggaagttg
      601 tcgacctccg gcatctcctt ggcgagctcc tcggggtagc gctggctcag gcagccggcg
      661 acgaccagcc gcttgccttt accggccgcc ttggcctgcg cgagctcgag gatcgtgtcg
      721 atcgactcct gcttggcgtc ctcgatgaag ccgcaggtgt tgacgaccag cgtgtcggcg
      781 tcctccgccg agtcgaccag ctcgtggccc tggtcgcgca ccacacccag catgatctcc
      841 gtgtcgacct ggttcttggg acagccgagc gagacgaagt agaccttctt gccggacgaa
      901 ttatgcggga tgggggcggt catgcgggcc gggggaggga agcacgtcca ccgagagggg
      961 tcaacgccgc gacccccatg accttgcgca cgggtgtcac atcttcttgc aaccaccgcc
     1021 ggccgccgcg aggcccgccg ctcgcagctc acgtccggtc gggccctcgc cgagcgcggc
     1081 gcgccgctcc ggcccgtgct ccgggctcag cacgtcgcgc agatacgccg cgtactcgaa
     1141 ggtgtccgag tgttcgggcg tgtggcacga ctggcacacc tcgacgctcg cgtcgcggcg
     1201 gatcgcgttg gccttgccgc gtttctccgg catcgccacg tgcgcgctcc cggggccgtg
     1261 acactgctcg cactggacgt tctgcagtcc cgcgttctcc accacttcgg atcctcccgg
     1321 ctcgcgaaag ccggtgacgt ggcagccgac gcagctcagg tcgaactgct tgttggcctt
     1381 caccagggtc gcgtacgcgt caccgtggcg ggtcttcgcc cagtgcgccg cctcgtcggc
     1441 gtggcacgcc tcgcattgtt cactgccgac gtagccggcc tggcccttcg tcggggccgg
     1501 cgcgcgcacg ccggcgaagc gttgctggtt cttcgcggcg acccaattgt cgtagctgtg
     1561 cagggccttg gccgcgtcgg ggtcgacggc gaggtgacac gagaccttgg tctgcgcgac
     1621 ggtgacgacg accgcgccgc tgaccggggc cggcttctcg atgctggccg ccagcgcggc
     1681 gcgctcctgc tcgaggcggg cgacgaaggc cttgtcggcg ctgacgtcgg cgcggaactt
     1741 ctcgaggcgg gcgtcgaggc gcgcgagctc gcggcgctgc gtctccaccg tcggcacgat
     1801 cgtccagctg tcgaccgccg gcagcttggc ggcgaccgcc ggatcgatct ccaggcgcag
     1861 gtgcgtcagc gtctgcgcct gctcgccggg ctcgaccacc caggtgttgc cgagccgcgc
     1921 cggcggtccg ccgaggcgcg tgcgctcgag gccgaccgga atgccgacga cgatcaggtc
     1981 gatgccctcg acctcgcgcg ccagctgctc ggcggtcgcg cgcgagccct gcaacagcac
     2041 caccgtcagc tccgcgcccg cctcgcgcat cgccttgatc tcgccgcgca gggcctccgc
     2101 gggggccgtc gccgccagcg agggcgcgag ccccggctcg accaccgccg tcacgccggc
     2161 gtcgatcgcc agcgcgtcgt cgccgaggcg gaccagacgg tgcctctgca caccacgcgc
     2221 gtcgagcttg cggctgttgg cgagcacgcg gggcagggga tatgtttcaa gggacaggtc
     2281 ggctttcggc gagctgaggt cggtcggtcc gagccccgag accagctgcg cgccgaggcc
     2341 ggcgaagcgg gtctgcaggg ccgcggcgcg ctgctcggcc tgggcccacg ccggctcgtc
     2401 cttggcggcc tcgggcccgg ccgggtcggc gaacaggaac gagcccggct cgagcacgag
     2461 gcgggtctgt ggccgcgagt tggcctcgat gtagccgaac gcgtactgca gcccgccgag
     2521 cggctcggtc gtgcagccgc acggggcgat cgtcccgagc acgcgggcga acgcgaagat
     2581 gtcgacggtg aggcgcggcg cggcggccgg ggtcggctcg gccgtggtcg cgggtcccgt
     2641 cgcgacgttg gtcgcgtcgg gcgcgccgca ggtggcgcag gccagggtgg ccgaggcgca
     2701 cagacccatg acggcgagca ggggcgatct cccgtggggg cgaccaagaa gaaggttggg
     2761 gcgggacaaa agcatgatgg ggaccgaccg cgccggggcg cagggcgaag cgaggtgaca
     2821 gcttaatcgc cgcaagaacc cgcgccgggc tgtgatggtg cgacgaagcg cacggacccg
     2881 ccagtcgacg aatcgaccag gacgcgctgc caggctttat aaggaccgct cgcacggtgt
     2941 ggagccggcg ggtgcagcgg tcccatcgtc tcatggccgc ggctgccgcg ctccggatgc
     3001 atcggctccg acttgaccgc gggtaccgcg gcagttagcc ttcgcaccct ttcgcccccc
     3061 gctctttcga gatacgcagg acagcaagac tcatggccgt caagatcagg ctcgcccgga
     3121 tgggctccaa gaagcgcccc tactaccgca tcgtcgctgc cgacgctcgc agcccccgcg
     3181 atggccggtt cctcgagcgc atcgggacgt acgaccccca cgccgagacc gtcgtcgtca
     3241 acgaggcacg cgtcagctac tggcaaggcg tgggcgcgca cccgacgatc accgtcgctc
     3301 gcctcctccg caaggcccgc aaggccgcca aggggaccac ccccgccgcc aaggcctgag
     3361 tcggccgacc tcggtcggtc gcctcggtcg ttcttcgacc gtgcagccgc aactcgatgc
     3421 cgggagcccg gaccctcgtg gtccgctctc tggtgtcgaa ccacggcctc acatcactgg
     3481 gaccacagcc gcgtccgacc atcggggccg ccttcgcgct cgcgagagcg ctcgcaggcc
     3541 cattttgccg agccgcgccg ggtgtgaaat gctgtacggg tcgtgatgcc gcgacccgtt
     3601 cctctccgcg cccgttctcc cgcgcctgct cgcacctctg gaggcatcca tgtcgtcgtc
     3661 gcccaacatc tccctcaagg aactcatcga gtacctcgca cgtgctctcg tcgaccaccc
     3721 caatgaggtg agcgtctccg aggtcgccgg cgaacagacc atcgtgctcg agctcaaggt
     3781 cgccaaggac gacctcggca aggtcatcgg caagcaggga cgcacggtca aggcgatgcg
     3841 cgccctgctg agcgccgcct cggccaagct gcgcaagcgg gccgacctcg agatcctcga
     3901 gtaagccgtg tcggccttca aacccgacgc ccggacccct gtcgtcggct cggcgacccg
     3961 ccagacgtcc ggcgcaccgt cgccggagac cctcgcggtc gccgtcgtcg tcggtgccca
     4021 tggcatccgc ggctgggtcc gcgtccatct ccacgacccg aagtcgaggg ccctgcgccc
     4081 gggcctgcgg gtcaccctgc acggccccga gggcgagcgg ccgagca
//
LOCUS       DASWNJ010000796         3211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_262818, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000796 DASWNJ010000000
VERSION     DASWNJ010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262818"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1709
                     /locus_tag="VGB85_13410"
     CDS             <1..1709
                     /locus_tag="VGB85_13410"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0135078.1"
                     /translation="QAGPAAAPRSVATEAATGVPRRADETVDPLVGTILADRYRIDRR
                     LGAGGMGAVYAGFHVTLNKRIALKVVQPRLGGDATIAQRFVQEARAASAIGHEHIVQI
                     TDFGRTADDAAFLVMEYLEGEDLSETLRREGPLPWQRVVHIGEQIAWALAAAHRQGIV
                     HRDIKPANCYRVPREHDDDFIKVLDFGLAKVLAEQRDGESLTGTGMLLGTPGYIAPEL
                     YRGLKADHRVDIYALGALMHKLLTGELPPMASGSGAADFMRNANAPGAVVAVLKGALA
                     ESPDERYGTAQELANALRAIPRVADDAPTQLGQVDGSRTQITGGQPRVDPSSAQAITL
                     REPTPSTFPSLFTVERSGDRVRFYFGPPLIVLLIGLVGALVWYLTRPVPVLVWAQENF
                     KTRQAGADAGKLINNPGPGAAVAAVQAAPPPPEPAVTPATPPPPELPPEPPAPAEPTT
                     PTTPTTPVAPDPTPDPKDMPPGTATRAPVVPQKGTPEGLGRCLTRKCGGDTLAYTVEL
                     AWKADAEGKPVRKSITAKVTNPEVRDCAEKSLRQCPLLWEPDASYTETITIGMRATKK
                     TP"
     gene            1828..2280
                     /locus_tag="VGB85_13415"
     CDS             1828..2280
                     /locus_tag="VGB85_13415"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY0135079.1"
                     /translation="MTSAAVFDRPAFAASSVTAPTSIPAGPSRRAVLAGRILGAFVVL
                     FLGFDLTLKLASVPMAIESTTELGYPADVLFPIGLIQLACLALYLIPRTAPLGALLWT
                     GYLGGAIATHVRVGNPLFSHTLFPIYVAAMLWGSLWLRDRRVRGLLAR"
     gene            2340..>3211
                     /locus_tag="VGB85_13420"
     CDS             2340..>3211
                     /locus_tag="VGB85_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135080.1"
                     /translation="MKGVEIVEDQAYFSGTTAELQEALTRGLLNRCGSLVVERKLIAL
                     PPEIGRLKKLRELILDTDTLQTIDPGLFQCAGLVKLVVLSNQLKLLPAGGWAKLKSLE
                     QLVLTTSNALRELPADIGDAPRLGGDFDLRAQTKLAPLPASFARLAGVTLLRLPPGLP
                     APDPIAGMKGLRELGLSAVDTIPADLGALAELRFLDLTECPITTLPEDLGNAKLMHTL
                     ALARTKLTRLPESIGELSALRELDLSETPLEALPESIGRAPLQRLRLQQTAITRLPDS
                     LATPAGDLRIFLPR"
BASE COUNT          456 a         1340 c          982 g          433 t
ORIGIN      
        1 cgcaggcggg cccggctgcc gcgccgcgct cggtcgccac cgaggccgcc accggcgtcc
       61 cgcggcgcgc cgacgagacc gtcgacccgc tggtcggcac gatcctcgcg gaccgctacc
      121 gcatcgaccg tcgcctcggc gccggcggca tgggtgcggt gtacgccggc tttcacgtca
      181 ccctcaacaa gcggatcgcc ctcaaggtcg tccagccgcg gctcggcggc gacgcgacga
      241 tcgcccagcg cttcgtacag gaggcccgcg ctgcctcggc gatcggccac gagcacatcg
      301 tccagatcac cgacttcggc cgcaccgccg acgacgccgc cttcctggtg atggagtacc
      361 tcgagggcga ggacctctcc gagaccctgc gccgcgaggg cccgctcccg tggcagcgcg
      421 tcgtccacat cggcgagcag atcgcctggg ccctcgccgc cgcgcaccgc cagggcatcg
      481 tccatcgcga catcaagccc gccaactgct accgcgtccc ccgcgagcac gacgacgact
      541 ttatcaaggt cctcgacttc ggcctcgcca aggtcctcgc cgagcagcgc gacggcgagt
      601 cgctcaccgg caccggcatg ctcctgggca cccccggcta catcgccccc gagctgtacc
      661 gcggcctcaa ggccgaccac cgcgtcgaca tctacgcgct cggcgccctg atgcacaagc
      721 tgctgaccgg cgagctgccg ccgatggcga gcggcagcgg tgccgccgac ttcatgcgca
      781 acgccaacgc cccgggcgcc gtggtggcgg tcctcaaggg cgccctcgcc gagtcacccg
      841 acgagcgcta cggcaccgcc caggagctgg cgaacgccct gcgcgcgatc ccccgggtcg
      901 ccgacgacgc gccgacccag ctcggccagg tcgatggctc gcgcacgcag atcaccggcg
      961 gtcagccacg cgtcgacccc agcagcgccc aggcgatcac gctgcgcgag ccgaccccct
     1021 cgaccttccc ctccctcttc accgtcgagc gcagcggcga ccgtgtccgc ttctatttcg
     1081 gtcctccgct gatcgtcctc ctgatcgggc tcgtcggcgc cctcgtgtgg tacctgaccc
     1141 gccccgtccc ggtcctcgtg tgggcgcagg agaacttcaa gacccgccag gccggcgcag
     1201 acgccggaaa gctgatcaat aaccccggac ccggcgccgc cgtggccgcg gtccaggccg
     1261 ccccgcctcc gcccgagcct gccgtcacgc ccgccacccc gccgccgccc gagctcccgc
     1321 ccgagccccc ggcgcccgcc gagccgacca caccgaccac cccgaccacc cccgtcgcgc
     1381 ccgacccgac acctgacccg aaggacatgc ccccggggac agccacgcgc gccccggtcg
     1441 tcccgcaaaa gggcaccccc gagggcctcg gccgctgcct gacgcgcaag tgcggcggcg
     1501 acactctcgc ctacaccgtc gagctggcct ggaaggccga cgcggagggc aagccggtgc
     1561 gcaagtcgat caccgccaag gtgaccaacc cggaggtccg cgactgcgcc gagaagagcc
     1621 tgcgccagtg cccgctgctg tgggagcccg acgcgtcgta caccgagacg atcacgatcg
     1681 gcatgcgcgc caccaaaaag accccctgag ggccccggga aacggcactc gctttttccc
     1741 gaaagatcgc cgggtgaaaa aagtgctcgc cgaccgatga tcgacgccgg gcccggccgt
     1801 ccaaggggta cataccggag cccccccatg accagcgccg ccgtcttcga tcgtcctgcc
     1861 ttcgccgcga gctcagtcac cgcgcccaca tcgatccccg ccgggccctc gcgccgcgcc
     1921 gtcctggccg gtcggatcct cggcgcattc gtggtgctgt tcctcggctt cgacctgacg
     1981 ctcaagctcg cctccgtgcc gatggcgatc gagagcacca ccgagctcgg ttatccggcg
     2041 gacgtcttgt tcccgatcgg cctgatccag ctcgcctgtc tcgcgctcta cctgatcccc
     2101 cgcaccgcgc cgctcggcgc gctgctgtgg accggctacc tcggcggtgc gatcgccacc
     2161 catgtccgcg tcggcaaccc gctgttctca cacaccctgt tcccgatcta cgtcgcggcg
     2221 atgctgtggg gcagcctgtg gctgcgcgac cgccgggtcc gcggcttgct cgcccgctga
     2281 ggccccgtgc ccggcgatcg cgcttgcgcc cgggcgcctg cccgtgcttt gtgaccgcca
     2341 tgaagggcgt cgagatcgtc gaggaccagg cttacttctc cggcaccacc gcggagctgc
     2401 aggaggcgct cacccgcggc ctgctcaacc gctgcggctc gctcgtcgtc gagcgcaagc
     2461 tgatcgcgct gcccccggag atcgggcgcc tcaaaaaact gcgcgagctg atcctcgaca
     2521 ccgacaccct gcagacgatc gaccccggcc tgttccagtg cgccggcctc gtcaagctcg
     2581 tcgtgctctc caaccagctc aagctgctcc cggccggcgg ctgggccaag ctcaagtcgc
     2641 tcgagcagct cgtgctgacc acctcgaacg cgctgcgcga gctcccggcc gacatcggtg
     2701 atgcgccgcg gctcggcggc gacttcgacc tgcgcgcgca gaccaagctc gccccgctgc
     2761 ccgcctcgtt cgcgcgcctc gccggcgtca ccctgctgcg cctgccgccc ggtctgcccg
     2821 cgcccgaccc gatcgccggc atgaagggcc tgcgcgagct cggcctgagc gccgtcgata
     2881 cgatccccgc cgacctcggc gcgctcgcgg agctgcgctt cctcgacctc accgagtgcc
     2941 cgatcaccac gctgcccgaa gacctcggca acgcgaagct catgcacacc ctcgccctcg
     3001 cgcgcaccaa acttacgcgc ctgccggagt cgatcggcga gctgtccgcg ctgcgcgagc
     3061 tcgacctcag cgagaccccg ctggaggccc tcccggagtc gatcggccgc gccccgctgc
     3121 agcgcctgcg cctccagcag accgcgatta cccgcctgcc cgacagcctc gccaccccgg
     3181 ccggcgacct gcgaattttc ctcccacgcg a
//
LOCUS       DASWNJ010000797         2966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_398613, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000797 DASWNJ010000000
VERSION     DASWNJ010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398613"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VGB85_13425"
     CDS             <1..346
                     /locus_tag="VGB85_13425"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HEY0135081.1"
                     /translation="PTHLAWQGTAFTSTGRVKVSRELQADRDWPLDLTCGCPTCASFT
                     RAYIHHLFKCGEPLGPRLLSIHNLYHYQQIMSEARGHIERGTYAAYARAKIAAIDVHE
                     HSGRRIGAAKES"
     gene            343..1146
                     /locus_tag="VGB85_13430"
     CDS             343..1146
                     /locus_tag="VGB85_13430"
                     /inference="COORDINATES: protein motif:HMM:NF017266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MnmC family methyltransferase"
                     /protein_id="HEY0135082.1"
                     /translation="MSCEVVTTRDGARAILDRGTGEVMHPVVGPAIESMRLYCEPARL
                     GARLQTGAGPLVLLDVGLGAGSNAIAAWHISEDRVGEGRRLEIVSFDHSLEAMQLALQ
                     HPEDFGFEGGAGEAARAVLQQGCHETRRTRWRVVLGSLPAVLQAEPAGGADVVFWDPF
                     SPRANPELWTVGAFTALRRCCRAGATVHTYSGATAVRSGLLLAGFAVGVGAATGEHKA
                     ATCAALELADLEQPLDRRWLARLGRSTAGFPSDAPVDALARIAALPQFA"
     gene            complement(1151..1741)
                     /locus_tag="VGB85_13435"
     CDS             complement(1151..1741)
                     /locus_tag="VGB85_13435"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY0135083.1"
                     /translation="MSSPTHYLVIDLEATCSDDESIPREEMEIIEIGAVLVDATSLTP
                     QAEFQTFIKPMRHKILKPFCTELTTITQADVDRAPRFPQAILKLGEFIKGKDALFCSW
                     GGYDRNQLRRDAQHHRVKLPLGERHMNIKEAFSRRLGESRQYGTGQALRRLGLSFTGT
                     QHRAIDDARNIARLLPFVLGATEPARVAAGGREARR"
     gene            complement(1874..2098)
                     /locus_tag="VGB85_13440"
     CDS             complement(1874..2098)
                     /locus_tag="VGB85_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135084.1"
                     /translation="MSCSARTQTTGPAVVIDWQTAPLAAVFFTSQVGDEYEHEHDGRL
                     LTGVVLRRHLVELRDGALVAICSDAVTTSR"
     gene            2553..>2966
                     /locus_tag="VGB85_13445"
     CDS             2553..>2966
                     /locus_tag="VGB85_13445"
                     /inference="COORDINATES: protein motif:HMM:NF012286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEY0135085.1"
                     /translation="MNSETPESIDTIETIDHRIAVVGCGRVGATSAYALMLRGVAREL
                     VLVDLDHGLAEGEAADLQHAVPMGRPVRVWAGDYKDAARAAIVVIAAGVSSKPGESRL
                     ELLGRNVVVVRECMKQLLAEGFKGIVLMTTNPVDVL"
BASE COUNT          432 a          908 c         1169 g          457 t
ORIGIN      
        1 gccgacgcac ctcgcgtggc aaggcacggc gttcacgtcg accgggcggg tcaaggtgtc
       61 gcgggagctg caagcggacc gcgactggcc gctcgacctc acctgcgggt gcccgacctg
      121 cgcgagcttc acgcgggcat acattcacca tctttttaag tgcggagagc cgctcgggcc
      181 gcggctgctg tcgatccaca atttatatca ttatcagcag atcatgagcg aggcgcgcgg
      241 gcacatcgag cgcgggacct acgccgcgta cgcgcgggcg aagatcgcgg cgatcgacgt
      301 ccacgagcac agcgggcgca ggatcggggc ggcgaaggag tcatgagctg cgaggtcgtg
      361 acgacgcgcg acggggcgcg ggcgatcctc gatcgtggca ccggcgaggt gatgcacccc
      421 gtcgtcggac cggcgatcga gtcgatgcgg ctgtattgcg agccggcgcg gctcggggcg
      481 cggctgcaga cgggcgcggg gccgctggtg ctgctcgacg tcgggctcgg cgcggggtcc
      541 aacgcgatcg cggcgtggca catctcggag gatcgggtgg gcgaggggag gcggctcgag
      601 atcgtcagct tcgatcatag cctcgaggcg atgcagctcg cgttgcagca tccggaggac
      661 ttcggcttcg agggcggcgc gggggaggcg gcgcgggccg tgttgcagca gggctgccat
      721 gagacccggc gtacgcgctg gcgcgtagtt ctgggcagct tgcccgccgt attgcaggcg
      781 gagccggcgg gcggcgccga cgtcgtgttc tgggatccat tctcgccgcg ggcgaacccg
      841 gagctgtgga cggtgggggc gttcacggcg ctgcggcgct gctgtcgggc gggcgcgaca
      901 gtgcatacat acagcggggc gaccgcggta cgctcggggt tgttgttggc cggcttcgcg
      961 gtcggggtcg gcgcggcgac gggagagcac aaggctgcga cctgcgcggc gctcgagctc
     1021 gcggatctcg agcagccgct cgatcgccgg tggctcgcgc ggctcgggcg gtcgacggcc
     1081 gggtttccgt cggacgcgcc cgtggacgcg ctggcccgga tcgccgcgct gccgcagttc
     1141 gcctgagcgg tcatcgccgg gcctcgcgcc cgccggcggc cacccgcgca ggctcggtgg
     1201 cgccgagcac gaagggcagc aggcgggcga tgttgcgggc gtcgtcgatc gcgcggtgct
     1261 gggtccccgt aaacgagagc ccgagccggc gcagcgcctg gccggtgcca tactggcgcg
     1321 actcgccgag gcgccgcgag aacgcctcct tgatgttcat gtgacgctcg ccgagcggga
     1381 gcttgacgcg gtgatgctgg gcgtccctgc gcagctgatt acggtcgtag ccgccccacg
     1441 agcagaacag ggcgtccttg cccttgatga attctcccag cttgaggatc gcctggggga
     1501 agcgcggcgc gcggtcgacg tccgcctggg tgatcgtggt cagctcggtg caaaacggct
     1561 tgaggatctt gtgccgcatc ggtttaataa atgtttggaa ctcggcctgc ggggtgagcg
     1621 aggtggcgtc gacgagcacg gcgccgatct cgatgatctc catctcctcg cggggaatgg
     1681 actcatcgtc gctgcaggtg gcctcgaggt cgatgacgag ataatgggta ggtgaagaca
     1741 tgccggcagt atggtcgcga acggcgtcgg cgagccggac acgatcccgg acaccgcggg
     1801 cgcgatcatg gccaggcggg cagtgggctg cgcgggccga cgctcaccag ccacagttga
     1861 tgcagcgggc gcgtcagcgc gacgtagtga cggcatcgct gcatatcgcg acgagcgcgc
     1921 cgtcgcggag ctcgacgagg tggcgccgca gcaccacgcc ggtgagcagg cggccgtcgt
     1981 gctcgtgctc gtactcgtcc ccgacctgcg aggtgaagaa gaccgcggcg agcggggcag
     2041 tctgccagtc gatgacgacg gccggtccgg tcgtctgcgt gcgcgccgag caggacatcg
     2101 cgctcgcccg cggcggtgcg gaccgcaggc gcgtggtacg gggctgttcg ggtcgtgaag
     2161 gtgtcggcga tcgccagctc ctcggcgacg agacggcgct cctcgggggt cagcggacgt
     2221 tgcatgaagc gatgcatgac acggcgaggc agacctacca gggcccggcg gtgaccttcc
     2281 tcgcggtgag ggggcgccac gctggcacga tggcgtggta gggtcggatc gatgggaccg
     2341 aggttggcaa aaccatggtc cgtggggcgg tccgaatgcg gccacgcgcg tgcgcatggt
     2401 gtcggtggag atcatgcctg gcgcgatttc tctgccgcgc gcgtgcgttt gaggtgatcc
     2461 cggtcaccgc ggtcgtacga gcccgcgtgg cgtggagccg ggaacgggtg tatcgcagtg
     2521 tcgtcgggtg cagcccggca cgcggagaca ggatgaatag cgagaccccc gagagcatcg
     2581 acaccattga aacgatcgat catcgcatcg ccgtcgtcgg ctgcgggcgt gtcggggcga
     2641 ccagcgcgta tgccctgatg ctgcgggggg tcgcgcgcga gctggtgctc gtcgacctcg
     2701 atcacgggct cgccgagggc gaggcggcgg acctgcaaca cgcggtgccg atgggccgcc
     2761 cggtgcgcgt ctgggccgga gactacaaag acgcggcgcg ggctgcgatc gtggtcatcg
     2821 cggcgggcgt cagcagcaag ccgggcgagt cgcggctcga gctgctcgga cgcaacgtgg
     2881 tggtggtacg cgagtgcatg aagcagctgc tcgcggaggg tttcaagggc atcgtgctga
     2941 tgacgaccaa cccggtcgac gtgctc
//
LOCUS       DASWNJ010000798         2627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_579968, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000798 DASWNJ010000000
VERSION     DASWNJ010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579968"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VGB85_13450"
     CDS             complement(<1..195)
                     /locus_tag="VGB85_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135086.1"
                     /translation="MSTLVACGNEDEAGAEALWDQVHADDYASWPHPPGYTERAPSSS
                     HGNEMEMFINDVLVAAFAAGT"
     gene            455..1675
                     /locus_tag="VGB85_13455"
     CDS             455..1675
                     /locus_tag="VGB85_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135087.1"
                     /translation="MPLDIFHERGTPLDRQVFTWKDIAGEPISKLDDDAFTRVRVIFM
                     HGIEQDSCRNKHIIHRMNADLRQVLARVRRVEHHQATLVNWLLSADHSPLEITIGYEQ
                     VAIEVTAHIAMVEPDPYIAQCHRFGLLEDFDHLYRYAALMDRLEGKDANNILQSYTDI
                     RPGRPTIVEHRHPDDDLRRHYDARTAAPLTKLNAMLLVGSEYQTRDYYLTVGPTFSDP
                     VARQLYAEIASIEEQHVTHYEALLDPNESVLEKWLLQEATEVYAYWSCYQQETNPRIK
                     LIWERFLSYELEHLRIAREVFERTEKRDAASVLPATLPDPISFTSHRAFIRETLAAEV
                     DLRANGLDFVPMSQESEASLAYRAQINSAGSPSQIVAAGYQWKPGGEITDPMIRDIAP
                     IPSAHLESRKEDAL"
     gene            1672..2433
                     /locus_tag="VGB85_13460"
     CDS             1672..2433
                     /locus_tag="VGB85_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135088.1"
                     /translation="MTTSDRPTDMGINRTGIALSPIDGPATVEGANAGLPSSSGSARA
                     IAEVRVQYAREAGPVGHMPPPVTLRGVASTAVEMIKGNKPTVLLDKLGERLAFERAGV
                     RMFDGLIAKYEASGSWAGGPTLEELVQFRNEEAQHFDLIKDVLLGLGADPTAMTPSGD
                     LAGVESMGIFQAINDPHNKLPEALHAHLIAELADVASWELLITLTEGLGHEEMGARFR
                     GALAHEIHHKESVMRWLAWHAEAAAKGESTTKSAS"
BASE COUNT          464 a          978 c          824 g          361 t
ORIGIN      
        1 cgtaccggcg gcgaacgccg cgacgaggac gtcgttgatg aacatctcca tctcgttgcc
       61 atgcgacgag ctcggcgcgc gctcggtata gcccggcgga tgcggccagc tcgcgtagtc
      121 gtcggcgtgg acctggtccc acagcgcctc cgcgccggcc tcgtcctcgt tgccgcacgc
      181 gacgagcgtc gacatcgtga cagcgatcag cagacacgaa gttcgtctgt acatcgcgtc
      241 actatacctg ctggcatgca tcgatcccag cccccgccgt tggctccacg cggagatcca
      301 cgggcgtaca cctccgcgcg gatcttggcg tcccctgcgc aagaaacgtg ctgcggcggc
      361 gtccacagcc gcagagccac gggtccggcc tgcgcgtggg cgtcaccggc gggaccgacc
      421 atacacccgg ggtcccacaa acaaggagca gcgagtgcca ctcgatatct ttcatgagcg
      481 gggaacgccg ctcgaccgcc aggtgttcac ctggaaagac atcgccggcg agccgatcag
      541 caagctcgac gacgacgctt tcacgcgcgt ccgcgtgatc ttcatgcacg gcatcgagca
      601 ggactcgtgc cgcaacaagc acatcatcca ccgcatgaac gccgacctgc gccaggtgct
      661 cgcccgcgtg cgtcgcgtcg agcatcacca ggcgacgctc gtcaactggc tgctgtccgc
      721 cgaccactcg ccgctcgaga tcacgatcgg ctacgagcag gtcgcgatcg aggtcaccgc
      781 ccacatcgcc atggtcgagc ccgacccgta catcgcgcag tgccaccgct tcgggttgct
      841 cgaagacttc gaccacctgt accgctacgc ggccttgatg gaccggctcg agggcaagga
      901 cgccaacaac atcctgcaga gctacaccga catccgcccc gggcgtccga cgatcgtcga
      961 gcaccgccac ccggacgacg acctgcgccg ccactacgac gccaggaccg ccgccccgct
     1021 gacgaagctc aacgcgatgc tgctggtcgg ctcggagtac cagacccgcg attactacct
     1081 gaccgtcggc ccgaccttct cggacccggt cgcgcgccag ctctacgccg agatcgcgtc
     1141 gatcgaggag cagcacgtca cccactacga ggcgctgctc gaccccaacg agagcgtgct
     1201 cgagaagtgg ctgctgcagg aggcgacgga ggtctacgcc tactggagct gctaccagca
     1261 ggagaccaac ccgcggatca agctgatctg ggagcgcttc ctcagctacg agctcgagca
     1321 cctgcggatc gcccgcgagg tcttcgagcg caccgagaag cgcgacgccg ccagcgtgct
     1381 gccggcgacg ctgcccgacc cgatcagctt caccagccac cgcgccttca tccgcgagac
     1441 cctcgcggcc gaggtcgacc tgcgcgccaa cggcctcgac ttcgtgccga tgagccagga
     1501 gagcgaggcc tcgctcgcct atcgggcgca gatcaacagc gccggctcgc cgtcccagat
     1561 cgtcgccgcc ggctaccagt ggaagcccgg cggtgagatc accgacccca tgatccgcga
     1621 catcgcaccg atcccaagcg cgcatctcga gagccgcaag gaggacgccc tgtgaccacc
     1681 agcgaccgac ccaccgacat gggcatcaac cgcaccggca tcgccctctc accgatcgac
     1741 ggccccgcca cggtcgaggg cgccaatgcc ggcctgccga gctcgtccgg ctctgcccgc
     1801 gcgatcgccg aggtccgtgt gcagtacgcc cgcgaggccg gacccgtcgg ccacatgccg
     1861 ccgcccgtca ccctgcgcgg cgtggcgtcc accgccgtcg agatgatcaa gggcaacaag
     1921 ccgaccgtgc tgctcgacaa gctcggcgag cgcctcgcct tcgagcgcgc cggcgtgcgc
     1981 atgttcgacg gcctgatcgc caagtacgag gcgagcggca gctgggccgg cggacccacc
     2041 ctcgaggagc tggtccagtt ccgcaacgag gaggcccagc acttcgacct catcaaggac
     2101 gtgctgctcg gcctcggcgc cgatccgacc gcgatgacgc cctcgggcga cctcgctggc
     2161 gtcgagagca tgggcatctt ccaggcgatc aacgacccgc acaacaagct gcccgaggcg
     2221 ctgcacgccc acctgatcgc cgagctcgcc gacgtcgcca gctgggagct gctgatcacc
     2281 ctcaccgagg ggctcggcca cgaggagatg ggggcccgct tccgcggcgc gctcgcgcac
     2341 gagatccatc acaaggagtc cgtgatgcgc tggctggcgt ggcacgccga ggccgccgcg
     2401 aagggcgagt cgacgaccaa gagcgccagc tgagccttcg tggtcgaaca agagccccga
     2461 tgcgcgtcgc atcggggctc ttttgtcagc cgacgggatt ggaaagatcc tcgtcgttgc
     2521 cgagcacggg gagccgccgg ccgcccgtga tcggcgtcgc gccgtcctcg ctgaagaact
     2581 ggcgcgtcca cgaccggaag cgcacgatcg ggccgtcggc gtcgcac
//
LOCUS       DASWNJ010000799         1705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_650598, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000799 DASWNJ010000000
VERSION     DASWNJ010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650598"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VGB85_13465"
     CDS             <1..669
                     /locus_tag="VGB85_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135089.1"
                     /translation="RVSLSVYVSGSALQSLSLANLTSVGYDTYFDKNPALVSVDLPAL
                     AAVCHQVYFDHNDLLASVNLPGLVTTGDDAAFLRFKPPSLSLPALESVGGSLMFYQSS
                     LVTVDLAKLVTVDGDLYLDHNTSLTNVGPSELTSVGGIFYADHNDALVSFDLPALVTI
                     GDYMYVTNNGLSKLGLAGLETVAGYGYFGNNAKLPACSVTMLGAMFGGMVTNMGNNGV
                     GPCE"
     gene            1000..>1705
                     /locus_tag="VGB85_13470"
     CDS             1000..>1705
                     /locus_tag="VGB85_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135090.1"
                     /translation="MWMALGTGLCACGDSGTATETAASSTGASTGTSVATTDVNTNGP
                     PTTGASNTDTPTSGNSEGMTEAVTSTGTSGSTGATTTEGVTTGPPPDMGPGCAETCLE
                     GVCVGDVCCPINQACTDSCCGAGEVCSFQQCVVPGADCVDATDCPADAYCEYTLGEIE
                     APPMCMGGVSLGTGKCLPTPPECAEGAEPVEGEPITCLPKCEVKPPADDFGIVLKAAW
                     GGQVVQPFASDIMMSPI"
BASE COUNT          254 a          576 c          608 g          267 t
ORIGIN      
        1 cgcgtctcgt tatcggtgta cgtctccggg agcgctctgc agagcctctc gcttgcgaac
       61 ctgaccagcg tcgggtacga cacctacttt gacaagaacc ccgcgctggt ctccgtcgac
      121 ctcccggccc tcgccgcggt ctgccatcag gtctacttcg accataacga cctgctcgcg
      181 agcgtgaacc tgcccggcct ggtgacgacc ggcgacgacg cggccttctt gcggttcaag
      241 ccgccgtccc tgagcctgcc cgcgctggag agcgtcggtg gcagcctgat gttctatcag
      301 tcctcgctgg tgacggtgga tctggccaag ctcgtgacgg tcgacggcga tctctatctc
      361 gatcacaaca cgtcgctgac gaacgtcggg ccgtccgagc tcaccagcgt cggtgggatc
      421 ttctatgccg accacaatga cgcgctggtc agcttcgacc tgcccgcgct ggtgaccatc
      481 ggcgattaca tgtatgtgac caacaacggc ctgagcaagc tcggcctcgc cggcctcgag
      541 acggtcgcgg gctacgggta cttcggcaac aacgcgaagc tgccggcctg ctcggtcacg
      601 atgctcggcg cgatgttcgg cggcatggtg accaatatgg gtaacaacgg cgtggggccc
      661 tgcgaatagg gcccggccgg tcgcgcagcg ccgggcgtgt gccggcgccg gttcactcgc
      721 aggcggcgac gtagctcgcg gttaccgcct gtcagtgtgg ctccgacgac gccgagtcgg
      781 gcaccgcgag cgcctcctcg accagcgagt tcatggcgcg cagcctgcac atcgaccgcg
      841 aggttgtcga ggcgccaagc cgcgcgcggc cccgcggccg atgtcgacgc ggtctccacg
      901 cgcgggtgat gttcggcgcc gcttcccctg cgcgcggcgc tgtggtctgc tggtgccatg
      961 catcgtggta tggggtcggg gtggggcgcg cgcgtgccca tgtggatggc tctcgggaca
     1021 ggtctgtgcg cctgcggtga cagcgggacg gcgacggaga ccgccgcgtc aagcacgggc
     1081 gccagcacgg gcacgagcgt cgcgacgacc gatgtcaaca ccaacggacc gccgacgacc
     1141 ggcgcgtcga acaccgacac gccgacatcc ggcaactcgg agggcatgac cgaggcggtc
     1201 accagtaccg gcaccagcgg gagcaccggc gcgacgacga ccgagggcgt gacgaccggt
     1261 ccgccgcccg acatgggccc gggctgcgcg gagacctgcc tcgagggcgt ctgcgtcggc
     1321 gacgtgtgct gcccgatcaa tcaggcgtgc accgatagct gctgcggggc gggcgaggtg
     1381 tgctcgttcc agcagtgcgt ggtcccgggg gccgactgcg tcgacgcgac cgactgcccg
     1441 gcggacgcgt attgcgagta cacgctcggc gagatcgagg cgccgccgat gtgcatgggc
     1501 ggcgtgtcgc tcggcaccgg caagtgcctg ccgaccccgc cggagtgcgc cgagggcgcc
     1561 gagccggtcg agggcgagcc gatcacgtgt ttgcccaagt gcgaggtcaa gccgccggcc
     1621 gacgacttcg ggatcgtgct caaggcggcg tggggcgggc aggtggtcca gccgttcgcg
     1681 agcgacatca tgatgtcgcc gatcg
//
LOCUS       DASWNJ010000800        12171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_156203, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000800 DASWNJ010000000
VERSION     DASWNJ010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 12171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12171
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156203"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..586
                     /locus_tag="VGB85_13475"
     CDS             224..586
                     /locus_tag="VGB85_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135091.1"
                     /translation="MALENNDPTTPEADKKTSKRKTAKKKAAKKTAGKKASKKTAAKK
                     TAKAGGAAGAKKTAKKAGGKKASKKTGAKKAAKKAGGAKRGASKKAGGAKAGGAKKTA
                     KKAGKKAGKKTAKRGTAA"
     gene            complement(848..1654)
                     /locus_tag="VGB85_13480"
     CDS             complement(848..1654)
                     /locus_tag="VGB85_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135092.1"
                     /translation="MPTPKLGILASDEKAFHAARETDPLSLVVLRSTLINPPRAAGAR
                     SVAENLKKLHPYASLVPYVWHLVTHGPEDHLRELATRALPGEPRRFGHLQSTPEVQDA
                     WQTSLRCAQAFGARYMLLRTPASFTPSGANHARLAAFAEARAAEGIELVWEAEGLWEP
                     AQTLSVARKLGMQTLIPAFDGAGRPFNGSLERRWLRVDGAGPTTKLRGILAESLVHSM
                     VHADVAAPVLFAGPRAYANMRAFAIELAIVEEDDDDLDDDDDEPDDETAE"
     gene            1728..2381
                     /gene="fsa"
                     /locus_tag="VGB85_13485"
     CDS             1728..2381
                     /gene="fsa"
                     /locus_tag="VGB85_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEY0135093.1"
                     /translation="MKLFIDTADIAEIREAMSLGLLDGVTTNPTLVAMTGKPMRDVLA
                     EICDLVDGPVSGEVLSTNYDEMMEEAHDLAAIAPNIVVKLPLIADGLRAVKELTSEGI
                     RTNVTLCFSPIQAMLAAKAGATYISPFVGRLDDVGHDGMDTVAQIVEIYNNYDFETEV
                     LVASVRSPTHVLEALRLGADVATIPLKVIHQLIKHPLTDVGLQRFIADAAKIPKGKH"
     gene            2413..3036
                     /locus_tag="VGB85_13490"
     CDS             2413..3036
                     /locus_tag="VGB85_13490"
                     /inference="COORDINATES: protein motif:HMM:NF014447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0135094.1"
                     /translation="MRKIRQHVNPLGEHFMRVRARPIDRPPQLAEDCRVEVELGCADA
                     EFSFQLATQNPTWLVAGLEIREPCLVRNRVRAQELGLENLRFAYSNLNVDLDRVFPPA
                     SVDRFHLLFPDPWFKKSHHKRRVIEPALVEVIADQLRPGGELHVASDVYEVALEIMEV
                     VEACPRLRNMIEPWSFWRGNPFAAESRREATTIARGQRVWRIRYALV"
     gene            complement(3033..3545)
                     /locus_tag="VGB85_13495"
     CDS             complement(3033..3545)
                     /locus_tag="VGB85_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135095.1"
                     /translation="MPMLADLDHPPRSRTRDMLIGAVFGLPVVGLLAWFLLPSLIHSV
                     TGGAGEYDQRLRAEDAYMQAVCGQVLDLGRDEQLCECVLAVEFPSLDCRQPFLRWSLD
                     RHHARCAEPEVRKPSLTFCSCIDTLAGMITKAEAEGDTPSAEIQRVGKCLELPDAFPL
                     PDIESLAPAP"
     gene            3732..4580
                     /locus_tag="VGB85_13500"
     CDS             3732..4580
                     /locus_tag="VGB85_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135096.1"
                     /translation="MKASFTSRIHCSVLAASLAALPCTEAQAQAPATKPATTAPAARP
                     ATASPAPAAGTSPTTTTAPPAAGTTTPTTGTTTTTPTGTTTPTTTTGTTGTTPPTTGT
                     PTDPNDPNRGVDGTGAVTAPGETPTDVSGETPAEPAPTPEPPPPEVTTPPAPPPEPVG
                     PERPPEPTVANGKYKAKGTGLMIAGGTLFGLGVIGVVTSYFLTKCDEPANSFACKNKQ
                     NNTFSVPATAAAALLGAVLLSVGIGYRVRYKKWENWKPENNPPKTAVYPTLLRGGAGV
                     GYTLNF"
     gene            complement(4653..5045)
                     /locus_tag="VGB85_13505"
     CDS             complement(4653..5045)
                     /locus_tag="VGB85_13505"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0135097.1"
                     /translation="MVDERTPDAQAREAHERRYMVRLGQRIRAVREERGLTQQAVAKS
                     AGIATDMVSRLENGHYTSPGLRTLLRIAEGMDVSLAMLLPDAPGVPSSAPESALKSKL
                     GALAHRAQPHELDLLVDIASVIVGRSQQ"
     gene            complement(5067..7181)
                     /gene="uvrB"
                     /locus_tag="VGB85_13510"
     CDS             complement(5067..7181)
                     /gene="uvrB"
                     /locus_tag="VGB85_13510"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEY0135098.1"
                     /translation="MARSSTSRSTPVSMSDPAEEWDLSNAVRPTIAADKPFRLASPFP
                     PAGSQPQAIKELVEGLRRGDRYQHLLGITGSGKTYTIANVINQVQRPTLILAHNKTLA
                     AQLYGEFKALFPDNAVEYFVSYYDYYQPEAYIPSTDTFIEKDSNINEQIDRLRHAATY
                     SLLSRRDVIIIASVSCIYGIGAAEAYYGMVATVAVGQTLDRDMLLRRLVEMQYERNDI
                     DFHRGTFRVRGDVVEVFPAYEDETAVRIEFFGDDVEAIKEIDALRGVTRGTMQRVTIF
                     PASHYVTPASQMRKAIQGIKEELQARLFELKEKIRLVEAQRLEQRTMFDLEMLEEMGF
                     CSGVENYSRHLTGRLPGEPPPTLLDYFPDDYLTIVDESHQTIPQVGSMYAGDRSRKTT
                     LVEHGFRLPSAMDNRPLKFEEWEQRCHQTIFLSATPAAYELGKTQGVIVQQIIRPTGL
                     LDPEIFVRPITNQVDDLLGEIRDRTSRGERILVTTLTKRMAEDLTEYYSDLGIKVRYL
                     HSDVETLDRVELLRGLRRGEFDVLVGINLLREGLDLPEVSLVAILDADKEGFLRETKS
                     LIQTCGRAARNLHGKVIMYADRITKSMQACMEETARRRIAQAEYNEIHGIIPRSTHAP
                     INSLEADAKPDAPPKKTKASDLPMGLGPLTDADLLPEELNGRIAELKAQMTELAKALR
                     YEEAAKLRDRLRVLEARKLEFI"
     gene            complement(7262..8275)
                     /locus_tag="VGB85_13515"
     CDS             complement(7262..8275)
                     /locus_tag="VGB85_13515"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEY0135099.1"
                     /translation="MTPELAIAVVMVIALIVYTLSAGADFGGGAWHLLASGPRADDQR
                     DLIDDAIGPIWEANHVWLILVVVLLFVCFPGAFAAISTALHIPLTVMLIGVVLRGSAF
                     AFQHAYTHAEHHDPAPRRWGRVFAVASLVTPVTLGVTLGALSVGIDVDPVTHLVRTDF
                     ISEWFAPFPFATGLLTLCLFAFLAAVYLAVEAAQDVELADIFRRRALLSALAVAAAAL
                     LTFIVAPPRLQHGLVASAWALALHLGTAAAAVAAITLLWRRRLRAARLAAMIQVALIV
                     LGWALAQHPHLVFPQLTILGAAAPRSVLVAVLIALGLGAVVLVPSLAYLFVVFKARPR
                     PET"
     gene            complement(8272..9573)
                     /locus_tag="VGB85_13520"
     CDS             complement(8272..9573)
                     /locus_tag="VGB85_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY0135100.1"
                     /translation="MTDLLAARAQMAMSLAFHIIFAMVGMAMPLLMVLAERRWLRHKD
                     PLDRTLARRWARGTAIMFAVGAVSGTALSFELGLLWPKFMGFAGPIVGMPFSLEGFAF
                     FFEAIFLGIYLYGWDRVAPRVHLFAGVMVLCSGVASAAFVLCANAWMNTPTGFTLKPD
                     GTLLDVDVWAAMWNPAALSEVLHMTLAAFASVGFAVAGIHAFMLLRSPGDAFHRRALG
                     LALAVGGVFACLQPLSGDLAAKQVAHYQPVKLAAMEGHWETARDVPLQLGGWPDEARE
                     TTDYALEIPYALSFMAFGDPHAEVRGLKDFPVNERPPVAIVHVAFQIMVACGMAMIAV
                     AAIAGFLKVRGRDLAKPWFLRIVVASAPLGLIAVEAGWTVTEVGRQPWVIQGVLRTQD
                     AVTPMPHLVVPFVTFSLLYLVLGVTVIVLLTRKVFTAPEDA"
     gene            9658..10335
                     /locus_tag="VGB85_13525"
     CDS             9658..10335
                     /locus_tag="VGB85_13525"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEY0135101.1"
                     /translation="MAALYAILDWPHAGGLDVVAAAAGLLGERPDGPGPRYLQLRCKG
                     ADTCTRLELLEQVAPMCRTAGVPLIVNDDLEAALARPGLVAGVHLGQQDLHEFGPQVT
                     WPERLAALRARAGAGFIIGVSTHDVGQVRASAALPVDHIGFGPVQATRSKAQPDPVVG
                     FDGLASACAASLHPVVAIGGLDAAGAVRAASAGAAMVAVIGALVAASPAEIRGRVVGL
                     ARALAAT"
     gene            10452..10622
                     /locus_tag="VGB85_13530"
     CDS             10452..10622
                     /locus_tag="VGB85_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135102.1"
                     /translation="MSDDDIVVVRKRVRTAPLPMIPREDIEPAPGLEGRLPVLFVIGF
                     LLLLAIQVALAL"
     gene            complement(10644..11849)
                     /locus_tag="VGB85_13535"
     CDS             complement(10644..11849)
                     /locus_tag="VGB85_13535"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY0135103.1"
                     /translation="MSTKKPLSRAIYIVAAKRTPFGTFGGALKDLTATDLAVIASKAA
                     LAAGNVSPEIVDAVCVGNVSHTSADALYMARHVGLRSGVPVPVPALTVNRLCGSGFQS
                     IVSGAQDILLGDAEIVLVGGTENMSQAPHAVRGVRWGTKFGQDGKLEDTLWSSLFDPY
                     AGCAMAITAENLAEKYKITREDCDTYGLRSQQGWAAAQDAGRFAAELTTVELKGRKGV
                     VQFAADEHARRDASLESMAKLPTAFKKDGVVTAGNASGICDGAAALVIASEEAVQKHG
                     LKPLARVVQWHVAGVDPTLMGIGPVPAIRGALVRAGLELGDLDLVEINEAFAAQYIAC
                     ERELGLDREKTNVNGGAIALGHPLGASGARITTHLVHELARRGGRYAVGSACIGGGQG
                     IALVLENMS"
BASE COUNT         1931 a         3994 c         4493 g         1753 t
ORIGIN      
        1 gcgctgcaca ctcccggctc gccaacctcc gcaaagacct ttcgaccgcg caagaaaata
       61 tgagccagct caccaaaacc cttcaaaagc cctcagtgtc ctacaagagt cgttcacctt
      121 cgtataattg agtaaaaaag atgttaattg gcgttgacac tacgtgagac ttatatgtaa
      181 ctactggtca gaaccgttgc ccctgaagga gggaccacac gttatggcgc tcgaaaacaa
      241 cgacccgacc acgcccgagg ccgacaagaa gacctctaag cgcaagaccg cgaagaagaa
      301 ggccgcgaag aagactgctg gcaagaaggc cagcaagaag accgccgcca agaagaccgc
      361 caaggccggt ggagccgcag gcgccaagaa gaccgcgaag aaggctggtg gcaagaaggc
      421 cagcaagaag accggtgcga agaaggctgc caagaaggcc ggcggcgcca agcgtggcgc
      481 ctccaagaag gccggcggcg ccaaggccgg tggggccaag aagaccgcga agaaggctgg
      541 caagaaggcc ggcaagaaga ccgccaagcg cggtaccgcg gcctgagggc ccgagcggtc
      601 tctccggagc cgctggccac atggccaaaa aatcgccgat ggtcacccga cctcggcgat
      661 tttcttgctt aacgggctgg gcgcaaacgc gcggcgaggg cgccggtcgc ggctgacggg
      721 cggcgccgcg acacgccgac gctgataccg cgccacctca ggcggcgcta cgcgcagagc
      781 ggacgctgac cgcgccggct caggcaggcg ctacgagcag agaaggcgcc gcgagtcgct
      841 cacgacctca ctcggcggtc tcgtcgtcgg gctcgtcatc gtcgtcatcc aggtcgtcgt
      901 cgtcctcctc gacgatcgcc agctcgatgg cgaaggctcg catgttggcg taggcgcgag
      961 ggccggcgaa cagcaccggg gcggcgacgt cggcgtggac catcgagtgg accagggact
     1021 cggcgaggat gccccgcagc ttggtggtag ggccggcgcc gtcgacccgc agccagcggc
     1081 gctcgaggct gccgttgaac gggcgaccgg cgccgtcgaa ggcggggatc agggtctgca
     1141 tgccgagctt gcgggcgacc gacagcgtct gcgccggctc ccacaggccc tcggcctccc
     1201 acaccagctc gatgccctcg gccgcgcggg cctcggcgaa agcggcgagg cgggcatggt
     1261 tcgcgccgct cggcgtgaag ctcgccgggg tgcgcagcaa catgtagcgg gcgccgaacg
     1321 cctgggcgca gcgcaggctg gtctgccagg cgtcctggac ctcgggggtg ctctgcaggt
     1381 ggccgaagcg tcgcggctcg ccgggcagcg cgcgcgtggc gagctcgcgc aggtggtcct
     1441 ccgggccgtg cgtgaccagg tgccagacgt agggcacgag gctggcgtag gggtgcagct
     1501 ttttgagatt ttcggcgacc gagcgggcac cggccgcgcg cggcgggttg atcagggtgg
     1561 agcggaggac gacgagcgag agggggtcgg tctcgcgggc ggcgtggaag gccttctcgt
     1621 cgctggcgag gataccgagc ttgggcgtgg gcatgcggac gcgcgcaggc tagtgcgaat
     1681 ggccagcatt gacaagcgcg ggccgccgtg tgctaccacc gcggcccatg aagctcttca
     1741 tcgacaccgc cgacatcgcc gagatccggg aggccatgtc gctcggcctg ctcgacggcg
     1801 tcacgaccaa cccgaccctg gtcgccatga ccggcaagcc gatgcgcgac gtgctcgccg
     1861 agatctgtga cctcgtcgac ggcccggtct cgggcgaggt gctgtcgacg aattacgacg
     1921 agatgatgga ggaggcccac gacctcgcgg cgatcgcccc gaacatcgtc gtcaagctgc
     1981 cgctgatcgc cgacggcctg cgcgccgtca aggagctgac cagcgagggc atccgcacca
     2041 acgtgacgct gtgcttctcg ccgatccagg cgatgctggc ggccaaggcc ggcgcgacct
     2101 acatctcgcc gttcgtcggt cgcctcgacg acgtcggcca cgacggcatg gacacggtcg
     2161 cgcagatcgt cgagatctat aacaattacg acttcgagac cgaggtgctg gtcgcctccg
     2221 tgcgctcgcc gacccacgtg ctcgaggccc tgcgcctcgg cgccgacgtc gcgacgatcc
     2281 cgctcaaggt catccaccaa cttattaaac acccgctgac cgacgtcggc ctgcagcgct
     2341 tcatcgccga cgcggcaaag atcccgaagg gcaagcacta ggcggacctg tcccgaaggg
     2401 caggtaatct tcgtgcgcaa gatccgtcag cacgtcaacc cgctcggcga gcacttcatg
     2461 cgcgtgcggg cgcggccgat cgatcggccg ccgcagctcg ccgaggattg ccgtgtcgag
     2521 gtcgagctcg gctgcgccga cgccgagttc agctttcaac tggcgacgca gaacccgacg
     2581 tggctggtcg ccggcctcga gatccgcgag ccctgcctcg tccgcaaccg ggtgcgcgcg
     2641 caggagctcg ggctcgagaa cctgcgcttt gcgtacagca acctcaacgt cgacctcgac
     2701 cgcgtgttcc cgccggcgag cgtcgaccgg ttccacctgc tgttccccga cccgtggttc
     2761 aaaaagagcc accacaagcg gcgggtgatc gagccggcgc tcgtcgaggt gatcgccgac
     2821 cagctgcgcc ccggcggcga gctgcacgtc gccagcgacg tctacgaggt cgcgctcgag
     2881 atcatggagg tcgtcgaggc ctgtccgcgt ctgcgcaaca tgatcgagcc gtggagcttc
     2941 tggcgcggta acccgttcgc cgccgagagc cggcgcgagg cgacgacgat cgcgcgcggg
     3001 caacgggtct ggcggatccg ctacgcgctg gtttaggggg ccggggcgag tgactcgatg
     3061 tcgggcagcg ggaaggcgtc cggcagctcg aggcacttgc cgacgcgctg gatctcggcg
     3121 ctcggcgtgt cgccctcggc ctcggccttc gtgatcatgc cggcgagggt gtcgatgcag
     3181 ctgcaaaagg tcagcgaggg cttgcggacc tcgggctcgg cgcagcgggc gtggtgacgg
     3241 tcgaggctcc agcgcaggaa cggctggcgg cagtcgagcg aggggaactc gacggcgagc
     3301 acgcattcgc agagctgctc gtcgcggccg aggtcgagga cctgaccgca caccgcctgc
     3361 atgtacgcgt cctcggctcg caggcgctgg tcgtactcac cggcgccgcc ggtgacgctg
     3421 tggatcaggc tcggcagcaa gaaccacgcg agcagtccga cgacgggcag gccgaagacg
     3481 gcgccgatga gcatgtcgcg ggtccgggac cgcggagggt gatcgaggtc tgcgagcatc
     3541 ggcatgggcg tttgcccagt gtaacgcggt tttagcgccc ggccagatcg gtgcaagtca
     3601 tgaagaagtg actgcgaggt ccgatcgcgg ggggaatgtg atggccgcgt ggcgccggct
     3661 tggggcttgt gaagccgcgg cccacgtacg atactcggcc gaagtcggcg gtcccgaacc
     3721 ctggaagcag catgaaggcc tcgttcacct cacggatcca ctgttctgtc ctggccgctt
     3781 ctctggccgc gctgccctgt accgaggcgc aggcccaggc accggcgacc aagcccgcca
     3841 cgaccgcccc ggcggccagg cccgccacgg cgagcccggc accggccgcg ggtacgagcc
     3901 cgacgaccac gacggcgccg ccggccgcag gcacgacgac gccgaccacc ggcacgacga
     3961 cgacgacgcc gaccggcacg acgacgccta ccaccacaac gggcacgacc ggcacgaccc
     4021 cgccgacgac cggcacgccg accgacccga acgacccgaa ccgcggtgtc gacggcaccg
     4081 gggccgtgac ggcgccgggc gagacgccga ccgacgtgtc cggcgagacg ccggccgagc
     4141 ccgcgccgac ccccgagccg ccgccgcccg aggtgaccac cccgccggcg ccgccgccgg
     4201 agcccgtggg tcccgagcgt ccgcccgagc cgacggtcgc caacggcaag tacaaggcca
     4261 agggcaccgg cctgatgatc gcaggcggca cgctgttcgg cctcggcgtc atcggcgtcg
     4321 tcaccagcta cttcctgacc aagtgcgacg agccggcgaa ctccttcgcc tgcaagaaca
     4381 agcagaacaa caccttctcg gtgcccgcga ccgccgcagc ggcgctgctc ggcgcggtgc
     4441 tgctctcggt cggcatcggc taccgcgtcc gctacaagaa gtgggagaac tggaagcccg
     4501 agaacaaccc gccgaagacc gccgtgtacc cgacgctgct gcgcggcggc gcaggcgtcg
     4561 gctacacgct caacttctga gcgatcggcg tgcgacgagc gcccggggcc gagaggccac
     4621 cgggcgttcg caattctctt ttttaagaat aatcactgct gcgagcggcc gacgatcacg
     4681 ctggcgatgt cgacgagcag gtcgagctcg tgcggctggg cgcggtgggc gagggcgccg
     4741 agcttcgact tgagggcgct ctccggggcc gagctcggca cgcccggggc gtcgggcagc
     4801 aacatcgcca gcgacacgtc catgccctcg gcgatccgca gcagcgtgcg caggccgggg
     4861 ctcgtgtagt ggccgttctc gaggcgcgag accatgtcgg tggcgatgcc ggccgacttc
     4921 gccacggctt gctgcgtcag gccgcgctcc tcccggaccg cgcggatgcg ctggcccagc
     4981 cggaccatgt agcgacgctc gtgggcctca cgggcctgcg cgtcgggggt gcgctcgtcg
     5041 accatgggaa gacgcgaaag gagcgatcag atgaactcga gcttgcgggc ctcgaggacg
     5101 cgcaggcggt cacgcagctt ggcggcctcc tcgtagcgca gggccttggc gagctcggtc
     5161 atctgggcct tgagctcggc gatccggccg ttgagctcct cgggcagcag gtcggcgtcg
     5221 gtgagcgggc cgaggcccat cggcaggtcg gaggccttgg tcttcttcgg cggcgcgtcg
     5281 ggcttggcgt cggcctcgag gctgttgatc ggcgcgtggg tgctgcgcgg gatgatgccg
     5341 tggatctcgt tgtactcggc ctgagcgatg cggcggcggg cggtctcctc catacatgct
     5401 tgcatgctct tggtgatgcg gtcggcgtac atgatcacct tgccgtggag gttacgtgcc
     5461 gcgcggccgc aggtctggat caggctcttg gtctcgcgca aaaagccctc cttgtcggcg
     5521 tcgaggatcg ccaccaggct gacctccggg aggtcgaggc cctcgcggag caggttgatg
     5581 ccgacgagca cgtcgaactc gccgcgtcgc aggccgcgca gcagctcgac gcgatcgagg
     5641 gtctcgacgt ccgagtggag gtagcggacc ttgatgccga ggtcgctgta gtactcggtc
     5701 aggtcctcgg ccatgcgctt ggtcagcgtg gtgacgagga tgcgctcgcc cctggaagta
     5761 cgatcgcgga tctcgccgag caggtcgtcg acctggttgg tgatcgggcg gacgaagatc
     5821 tccgggtcga gcaggccggt cgggcggatg atctgctgga cgatgacgcc ctgcgtcttg
     5881 ccgagctcgt aggcggcagg cgtggcgctc aggaagatgg tctggtggca gcgctgctcc
     5941 cactcctcga acttgagcgg gcggttgtcc atcgccgagg gcaggcgaaa gccgtgctcg
     6001 acgagggtgg tcttgcggct gcggtcgccg gcgtacatcg agccgacctg cgggatcgtc
     6061 tggtgcgact cgtcgacgat cgtcaggtag tcgtccggga agtagtcgag cagggtcggg
     6121 ggcggctcgc caggcaggcg gccggtcagg tggcgcgagt agttctcgac gcccgagcaa
     6181 aagcccatct cttcgagcat ctcgaggtcg aacatcgtgc gctgctcgag gcgctgggcc
     6241 tcgacgaggc ggatcttctc cttgagctcg aacaggcggg cctgcagctc ttctttaatg
     6301 ccctggatcg ccttgcgcat ctgcgaggcg ggcgtcacgt agtggctcgc cgggaagatc
     6361 gtgacgcgct gcatcgtgcc gcgcgtgacg ccgcgcaggg cgtcgatctc cttgatcgcc
     6421 tcgacgtcgt cgccgaagaa ctcgatgcgc acggcggtct cgtcctcgta ggccgggaac
     6481 acctcgacga cgtcgccgcg cacccggaag gtcccgcggt ggaagtcgat gtcgttgcgc
     6541 tcgtactgca tctcgaccag gcgacgcagc agcatgtcgc ggtcgagcgt ttggccgacc
     6601 gcgacggtgg cgaccatgcc gtagtaggcc tccgccgcgc cgatgccgta gatgcacgag
     6661 acgctggcga tgatgatgac gtcgcgccgg ctcagcagcg agtaggtcgc cgcgtggcgc
     6721 aggcgatcga tctgctcgtt gatgttggag tccttctcga tgaaggtgtc cgtcgacggg
     6781 atgtacgcct cgggctggta gtagtcgtag taggagacga agtactcgac cgcgttgtcg
     6841 gggaacagcg ccttgaactc gccgtacagc tgggcggcga gggtcttgtt gtgcgcgagg
     6901 atcagcgtcg gccgctggac ctggttgatg acgttggcga tcgtgtaggt cttgccgctg
     6961 ccggtgatgc cgagcaggtg ctggtagcgg tcaccgcggc gcaggccctc gacgagctcc
     7021 ttgatcgcct gcggctggct gccggccggc gggaacgggc tcgccaggcg aaacggcttg
     7081 tccgccgcga tcgtcgggcg gacggcgttg ctgaggtccc actcctcggc cgggtcgctc
     7141 atcgacacgg gggtgctgcg actggtgctg ctgcgagcca tggcgggcaa agatagcagc
     7201 gaggcccgcg cgggcgaggt cgcacctgcc gcgacttctc acgtttgttg tcaggtgttt
     7261 gtcaggtttc aggccgcggc cgggccttga agacgacgaa gaggtacgcg agcgagggga
     7321 ccagcacgac ggcgccgagg ccaagggcga tcagcacggc gacgagcacc gagcgcgggg
     7381 cggcggcgcc gaggatcgtc agctgcggga acacgaggtg cgggtgctgg gccagcgccc
     7441 agccgagcac gatcagcgcg acctggatca tcgccgcgag gcgggccgcg cggagcctgc
     7501 ggcgccacaa caatgtgatc gcggcgacgg cggccgccgc ggtgccgagg tgcagcgcga
     7561 gcgcccaggc ggacgcgacg aggccgtgtt gcaggcgcgg cggggcgacg atgaaggtca
     7621 gcagcgcggc ggcggcgacc gccagcgcgg acagcagggc gcggcggcgg aagatgtccg
     7681 cgagctcgac atcctgcgcg gcctcgacgg cgaggtacac cgcggcgagg aaggcgaaca
     7741 ggcacagggt cagcaggccg gtggcgaacg ggaagggcgc gaaccactcc gagatgaagt
     7801 cggtgcgcac gaggtgggtg acggggtcga cgtcgatgcc gacactgagc gcgccgagcg
     7861 tgacgccgag ggtgacgggg gtgacgaggc tggcgaccgc gaacacgcga ccccagcggc
     7921 ggggagcggg gtcgtgatgc tcggcgtggg tgtatgcgtg ctggaacgcg aaggccgagc
     7981 cgcgcaggac cacgccgatc agcatcacgg tcagcgggat gtgcagggcg gtcgagatcg
     8041 ccgcgaaggc gcccgggaag cacacgaaca gcagcacgac gacgaggatc agccacacat
     8101 gattggcctc ccagatcggg ccgatcgcgt cgtcgatcag gtcgcgttga tcgtccgcgc
     8161 gcgggcccga ggcgagcagg tgccaggcgc cgccgccgaa gtcggcgcct gcgctgagcg
     8221 tgtagacgat cagcgcgatc accatgacga ccgcgatcgc cagctcgggt gtcacgcgtc
     8281 ctccggggcg gtgaacacct tgcgcgtcag cagcacgatc acggtgacgc cgagcacgag
     8341 gtagagcagc gagaaggtca cgaagggcac gaccaggtgg ggcatcggcg tgacggcgtc
     8401 ctgcgtgcgc agcacgccct ggatcaccca cggctggcgg ccgacctcgg tgacggtcca
     8461 gccggcctcg acggcgatca ggcccagcgg cgcgctggcg accacgatcc gcaggaacca
     8521 cggcttggcg aggtctctac ctcgcacctt caaaaacccg gcgatcgcgg cgacggcgat
     8581 catcgccata ccgcaggcga ccatgatctg aaacgcgacg tgaacgatcg ccaccggcgg
     8641 gcgctcgttt actggaaaat ccttgaggcc gcggacctcg gcgtgcgggt cgccgaaggc
     8701 catgaagctg agggcgtagg gaatctcgag cgcgtagtcg gtggtctcgc gggcctcgtc
     8761 gggccagccg ccgagttgca gcgggacgtc gcgggcggtc tcccagtggc cctccatcgc
     8821 ggcgagcttg accggttggt agtgcgcgac ctgcttggcg gcgaggtcac cgctgagtgg
     8881 ttgcaggcag gcgaacacgc cgccgaccgc gagcgcgaga ccgagcgcgc ggcggtggaa
     8941 ggcgtcgccg gggcttcgca ggagcatgaa ggcgtggatg ccggcgaccg cgaagccgac
     9001 cgaggcgaac gcggcgaggg tcatgtgcag gacctcgctg agcgcggcgg ggttccacat
     9061 cgcggcccag acgtcgacat ccaacagagt gccgtcgggc ttgagggtga agccggtcgg
     9121 ggtgttcatc caggcgttgg cgcacagcac gaaggccgcg gaggcgacgc cggagcaaag
     9181 gaccatgacg ccggcgaaca ggtgcacgcg cggggcgacg cggtcccagc cgtagaggta
     9241 gatgccgagg aagatcgcct cgaagaagaa ggcgaagccc tcgagcgaga agggcatgcc
     9301 gacgatcggg ccggcgaagc ccatgaactt cggccacagc agcccgagct cgaacgacag
     9361 cgcggtgccc gagacggcgc cgacggcgaa catgatcgcg gtgccgcggg cccagcgacg
     9421 cgcgagcgtg cggtcgagcg gatctttgtg gcgcagccag cgacgctcgg cgagcaccat
     9481 caacagcggc atcgccatgc ccaccatcgc aaagatgatg tggaaggcca gcgacatggc
     9541 catctgcgcg cgggcggcga ggaggtcggt cacgcggggc tccgtgaagc agtgtgccgc
     9601 gccgtggcgc gccggtcact gcggcggcgg gccgcggggg cgtggtacaa gccggcgatg
     9661 gccgcgttgt atgcgatcct cgattggccc cacgcgggcg ggctcgacgt cgtcgcggcc
     9721 gctgccggcc tgcttggcga gcgccccgac ggtccgggtc cgcgatatct acagctgcgc
     9781 tgcaagggtg cggacacgtg tacgcgcctc gagctgctcg agcaagtcgc gccgatgtgc
     9841 cggaccgcgg gggtgccgtt gatcgtcaac gacgatctcg aggctgcgct cgcccggccc
     9901 gggctggtcg ccggcgtgca cctcggacag caggatctgc acgagttcgg tccgcaggtg
     9961 acctggcccg aaagacttgc ggccctgcgc gcgcgtgcgg gtgcgggctt catcatcggc
    10021 gtctcgaccc acgacgttgg tcaggtgcgc gcgagcgcgg cgctgccggt cgaccacatc
    10081 ggctttgggc cggtgcaggc gacgcgatcg aaggcgcagc cggacccggt cgtcggcttc
    10141 gacgggctgg cgagcgcgtg tgcggcctcc ctgcatcccg tggtggcgat cgggggcctg
    10201 gacgccgccg gggcggtgcg ggcggcgagc gcgggcgcgg cgatggtcgc ggtgatcggt
    10261 gcgctggtcg ccgcgagccc ggccgagatc cgcggacgcg tcgtcgggct cgcacgggcg
    10321 ctggccgcga cctagaccgg tctggactgc gttcgccgcg cggcggaccc tggccgccgc
    10381 gttaccctcg tcacttcgcg ggcgcgacct ttgccgctgc tctggcccgg cgactccgct
    10441 atcgtgatcg agtgtcggac gacgatatcg tggtggttcg caagcgcgtg cgaactgcgc
    10501 cgctgcctat gatcccacgc gaggacatcg agccggcgcc cggcctcgag ggccggctgc
    10561 cggtgttgtt cgtgatcggg ttcctgttgc tgctcgcgat ccaggtcgcg ctggcgctgt
    10621 agagcgccag cgcgtgcggg ctctcaggac atgttctcga gcacgagggc gattccctgg
    10681 ccgccgccga tgcaggccga gccgacggca taacggccgc cgcgacgggc cagctcgtgg
    10741 acgaggtggg tggtgatgcg ggccccggag gcgccgagcg ggtggccgag ggcgatcgcg
    10801 ccgccgttga cgttggtctt ctcgcggtcg aggccgagct cgcgctcgca ggcgatgtac
    10861 tgcgccgcga aggcctcgtt gatctcgacg aggtcgaggt cgccgagctc gaggccggcg
    10921 cgcacgaggg cgccgcggat cgcaggcacc gggccgatgc ccatcagcgt cgggtcgacg
    10981 ccggcgacgt gccactggac cacgcgcgcg agcggcttga ggccgtgctt ttgcaccgcc
    11041 tcctcgctgg cgatcacgag ggcggccgcg ccgtcgcaga tgcccgaggc gttgcccgcc
    11101 gtgaccacgc cgtccttctt gaacgcggtc ggcagcttgg ccatcgactc gaggctggcg
    11161 tcgcggcgcg cgtgctcgtc ggcggcgaac tggacgacgc ccttgcggcc cttgagctcg
    11221 acggtcgtca gctcggcggc gaagcggccg gcgtcctgcg cggcggccca gccctgctgc
    11281 gagcgcaggc cgtaggtgtc gcagtcctcg cgggtgatct tgtacttctc ggccaggttc
    11341 tcggcggtga tcgccatcgc gcagccggcg taggggtcga agagggagga ccacagggtg
    11401 tcctcgagct tgccgtcctg gccgaacttg gtgccccagc ggacgcctcg gaccgcgtgc
    11461 ggggcctggc tcatgttctc ggtgccgccg acgagcacga tctccgcgtc gccgagcagg
    11521 atgtcctgcg cgccgctgac gatcgactgg aagcccgagc cgcacaggcg gttgacggtg
    11581 agggcgggca ccgggaccgg cacgccggag cgcaggccga cgtggcgggc catatacagg
    11641 gcgtccgcgg aggtatgaga gacgttgccc acgcacaccg cgtcgacgat ctccgggctc
    11701 acgttgccgg cggcgagggc ggccttggag gcgatcaccg cgaggtcggt cgccgtcagg
    11761 tccttgagcg ctccgccgaa ggtcccgaac ggggtccgct tggccgcgac gatgtagatg
    11821 gctcgagaga ggggcttttt cgtcgacatc gccgcccagt atgcgcaccg gcttcggcgc
    11881 gccacggtgc gattcggttg agcatcctca cggtttgcgg cattttggag gcggttgccg
    11941 accgctcctc tcatcgtgtc cctcgccgtc gccctcatca gcgagcctgc ggcgacctcg
    12001 agcgcgcctg cgtccgcgaa cgacccgcgt gaccgggacc tgctgcggcg acttgaggtt
    12061 ccgcaaggac ccgaggtgcg cacgcacgtg acgcgacgcg agcgactgcg gcgctatctc
    12121 acccgcgagg tcgctcgctc cgcgcgccac ctcgaccacg gcgtgctcgg c
//
LOCUS       DASWNJ010000801         2353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_120201, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000801 DASWNJ010000000
VERSION     DASWNJ010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120201"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1522
                     /locus_tag="VGB85_13540"
     CDS             <1..1522
                     /locus_tag="VGB85_13540"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0135104.1"
                     /translation="GTALGQFAFSGPGQLEGKLHVAAHAARAGTAGVLRQDDVAAGTV
                     GFYDRYDDPTASAQSASAARGQVALELARKASAGGRTHFAVHAALVDFHSRENFTGYL
                     KRGMVMPDWVGRGDLIEQQNRDLGGGFRLSHRTGWWRPVKLFAAAIELGSSARIHRIT
                     QAQNLLQAPSNETWDRRVDAEVLATSVGMFADLDLRLGKYVQVRGGARADVLHYDVDD
                     RLGNFIAPYQVASHLVGYRRTATGVAAGPRAAVEVTPLPWLRTFVAYGQGYRSPQARQ
                     LEEGERAPFTKVHSVEGGVRLAPKWRRPVHLTAAGYGTFLANDLAFDPEEARLERIGP
                     TRRSGLVASLLARPWPWLTAAASVTYVHAVLTAPPPATVEDPNPAYVAGQLLPYVPPV
                     VVRGDLGVDKELARLGKHPLALRVGSGFSYLSARPLPYGQTSDPLYLLDASAGLRWSY
                     VELGVEAYNLLARRYAASVYSFVSDWGTRPVPSRLPAPHFAAGPPLTILGTLTLSF"
     gene            1525..2262
                     /locus_tag="VGB85_13545"
     CDS             1525..2262
                     /locus_tag="VGB85_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135105.1"
                     /translation="MSRRTAISALALLACSITLLASCGAILLACGSTGAEPFRYPLVA
                     TGLAAEPFAVGDWQVTLTRADVAVGPVYLCATAAASPELCAVAIGEFTEVAVVDALDP
                     AAQPLGDIDARPGDVRSAMLDYGISWFTTATAPAPLSALDHSARISGTATLDDISLEF
                     DATIDVTPPMRGSPALVGLHAVTDAPAEDSRLELRIDVRGWLADVDFDALAAIGPSHL
                     IKPGDDAHAAIAFAMTTQPPVFRWTGP"
BASE COUNT          289 a         1019 c          720 g          325 t
ORIGIN      
        1 cggcaccgcg ctcgggcagt tcgcgttctc cggcccgggc cagctcgagg gcaagctgca
       61 cgtcgccgcc cacgccgcgc gcgcgggcac ggccggcgtg ctgcgccagg acgacgtcgc
      121 ggccggcacc gtcggcttct acgaccgcta cgacgacccg accgccagcg cgcagtcggc
      181 ctccgccgcg cgcggccagg tcgccctcga gctcgcccgc aaggccagcg ccggcggccg
      241 cacgcacttc gccgtgcacg ccgcgctcgt cgacttccac tcgcgcgaga acttcaccgg
      301 ctacctcaag cgcggcatgg tcatgccgga ctgggtcggc cgcggcgacc tgatcgagca
      361 gcagaaccgc gacctcggcg gcggctttcg cctgtcccat cgcaccggct ggtggcgccc
      421 ggtgaagctc ttcgccgccg cgatcgagct cggcagcagc gcacggatcc accgcatcac
      481 ccaggcccag aacctgctgc aggcgccgag caacgagacc tgggaccgcc gcgtcgacgc
      541 cgaggtcctc gccaccagcg tcggcatgtt cgccgacctc gacctgcgcc tcggcaagta
      601 cgtgcaggtc cgcgggggcg cccgcgccga tgtcctccac tacgacgtcg acgaccgcct
      661 cggcaacttc atcgccccct accaggtcgc ctcgcacctc gtcggctatc gccgcaccgc
      721 caccggcgtc gccgcgggcc cgcgcgccgc cgtcgaggtc accccgctgc cgtggctgcg
      781 cacctttgtc gcctacggtc agggctaccg ctcgccgcag gcccgccagc tcgaggaggg
      841 cgagcgcgcc ccgttcacga aagtgcacag cgtcgagggc ggcgtccgcc tcgcgccgaa
      901 gtggcgccgc cccgtgcacc tcaccgccgc cggctacggc accttcctcg ccaacgacct
      961 cgccttcgac cccgaggagg ctcgcctcga gcgcatcggg cccacgcgcc gcagcggcct
     1021 cgtcgcctcc ctgctggccc gcccgtggcc gtggctgacc gccgccgcgt ccgtcaccta
     1081 cgtgcacgcc gtgctgaccg caccgccgcc cgcgaccgtc gaggacccca accccgccta
     1141 cgtcgccggc cagttgctgc cctacgtgcc accggtggtc gtgcgcggcg acctcggcgt
     1201 cgacaaggag ctcgcgcggc tcggcaagca cccgctcgcc ctgcgcgtcg gctccggctt
     1261 tagttacctg tccgcgcgtc cgctccccta cggccagacc tccgacccgc tgtacctgct
     1321 cgacgcctcg gccggcctgc gctggtcgta cgtcgagctc ggcgtcgagg cctacaacct
     1381 gctcgcccgc cgctacgccg ccagcgtcta ctccttcgtg tccgactggg gcacccggcc
     1441 ggtgccctcg cgcctgcccg ccccgcactt cgccgccggc cccccgctga cgatcctcgg
     1501 caccctcacc ctgtcctttt gaacatgtcc cgacgtacag ccatatccgc gctcgccctg
     1561 ctcgcctgca gcatcacact gctcgccagc tgcggcgcca tcctgctcgc ctgcggctcc
     1621 accggcgcgg agccgtttcg ctacccgctc gtggccaccg gcctcgccgc ggagccgttc
     1681 gcggtcggcg actggcaggt gacgctgacc cgcgccgacg tcgcggtcgg ccccgtgtac
     1741 ctgtgcgcca ccgcggccgc ctcacccgag ctgtgcgccg tcgcgatcgg cgaattcacg
     1801 gaagtcgccg tcgtcgacgc gctcgacccc gccgcccagc cgctcggcga catcgacgcg
     1861 cgacccggcg acgtgcgctc ggcgatgctc gactacggca tctcgtggtt caccaccgcg
     1921 accgccccgg ccccgctgag cgcgctcgat cactccgcgc ggatctccgg caccgccacg
     1981 ctcgacgaca tctcgctcga gttcgacgcc acgatcgacg tcaccccgcc gatgcgcggc
     2041 agcccggcgc tcgtcggcct gcacgccgtc accgacgccc ccgccgagga cagccgcctc
     2101 gagcttcgca tcgacgtccg cggctggctc gccgacgtcg acttcgacgc gctcgcggcg
     2161 atcggcccga gccacctcat caaacccggc gacgacgccc acgccgcgat cgccttcgcc
     2221 atgaccaccc agccgccggt ctttcgctgg accggcccgt gatcggagca ccatgcaagc
     2281 acgcatccct ctcgcactcc tgaccctgct caccgcctgc gacgccggca ccgggaccgt
     2341 gcaggtcttc gtc
//
LOCUS       DASWNJ010000802         1763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_226657, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000802 DASWNJ010000000
VERSION     DASWNJ010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226657"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..651)
                     /locus_tag="VGB85_13550"
     CDS             complement(37..651)
                     /locus_tag="VGB85_13550"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HEY0135106.1"
                     /translation="MELLGQSVLAWSLFAYLVAAIPMGVLVGLARGVDIRKLGSGNIG
                     ATNAVRVLGARWGLVVFVLDVLKAFLPVRLAMAGWALGGSEGALAVVALAATLGHIFP
                     VYLGFRGGKGVACALGVFAALMPASGLFAGILYLQTLALTRVSAIASLTAVNAMTLHL
                     WLSGAPGPYRWLAVGMAGLIWVRHRGNLREIARMAAERKRQAAP"
     gene            complement(705..1388)
                     /gene="hisIE"
                     /locus_tag="VGB85_13555"
     CDS             complement(705..1388)
                     /gene="hisIE"
                     /locus_tag="VGB85_13555"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF002747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HEY0135107.1"
                     /translation="MEGFDPAALWATLKTDGDGLVTAVVQDHLDGQVLMVGHMNAEAL
                     AATLQSGRVTFWSRSRACLWEKGESSGHTLELRGVAVDCDRDALLVHAEARGPTCHTG
                     ARSCFFTPVLGASAATVLDRLYVEICERRAGRGVTSATGKSYVRGLLDGGVPAIAAKL
                     HEEAGELVEALAAEDRQHIAREAADLLFHALVGLAHREVDLGDVLAVLAARMGRSGVD
                     EKAARPPRA"
     gene            complement(1400..>1763)
                     /locus_tag="VGB85_13560"
     CDS             complement(1400..>1763)
                     /locus_tag="VGB85_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEY0135108.1"
                     /translation="STGLEALRWCDHVAALGAGEVLLTSMDKDGTRDGYDLWITREVS
                     HRLPIPVIASGGVGQLEHLREGLAVDQGGADAALAASIFHFGLYTISQAKQYLHEHGI
                     PVRPQEAPRGVTISGGFG"
BASE COUNT          265 a          634 c          644 g          220 t
ORIGIN      
        1 agagcgtgcg ggtgatgcgt ggcttcaccg cgggcctcag ggggcggctt gccgcttgcg
       61 ctcggcggcc atgcgggcga tctctcgcag gttgccgcgg tggcggaccc agatcaggcc
      121 ggccatgccg accgcgagcc agcggtaggg acccggcgcg cccgacagcc acaggtggag
      181 ggtcatcgcg ttgacggcgg tcagcgaggc gatcgccgag acgcgggtca gcgcgagggt
      241 ctgtaagtac aaaatgcccg cgaacaggcc gctggcgggc atcaacgccg cgaacacccc
      301 gagcgcgcag gcgaccccct tgccgccgcg aaagccgagg tacaccggga agatgtggcc
      361 gagcgtcgcc gcgagggcga cgaccgccag ggcgccctcg gagccgccga gcgcccagcc
      421 ggccatcgcc aggcgcaccg gcaaaaacgc cttgagcacg tcgagcacga acacgacgag
      481 gccccacctg gccccgagca cgcgcacggc gttggtggcc ccgatgttgc cggacccgag
      541 cttgcggatg tcgacgcccc gcgcgaggcc gacgagcacg cccatgggga tcgccgcgac
      601 caggtaggcg aacaggctcc aggcgaggac gctttggcca aggagttcca cgtcgcggat
      661 cataaccgct ccgcggctcg cctggccaca ttaagccggg ggcttcaggc gcggggcggt
      721 cgggcggcct tctcgtcgac gccgctgcgc cccatgcgtg ccgcgagcac cgccagcacg
      781 tcgccgagat cgacctcgcg gtgggccagg ccgaccagcg cgtggaacag caggtcggcg
      841 gcctcgcggg cgatgtgctg gcggtcctcc gctgccagcg cctcgacgag ctcgccggcc
      901 tcctcgtgca gcttggcggc gatcgccggg acgccgccgt ccaggagccc gcggacgtag
      961 ctcttgccgg tcgcgctggt gaccccacgc ccggcccggc gctcgcagat ctcgacgtac
     1021 aaccggtcga gcacggtcgc cgcggaggcg ccgagcaccg gcgtgaagaa gcagctgcgc
     1081 gcaccagtgt gacaggtcgg gccgcgcgcc tcggcgtgga ccagcagcgc gtcgcggtcg
     1141 cagtcgacgg cgacgccgcg cagctcgagc gtgtgccccg agctctcacc cttctcccac
     1201 aggcaggcgc ggctgcggct ccaaaaggtc acgcgcccgc tctgcagcgt ggcggcgagc
     1261 gcctcggcgt tcatgtgccc gaccatcaac acctggccgt cgaggtggtc ctgcacgacc
     1321 gcggtgacga ggccgtcgcc gtcggtcttg agcgtggccc acagggccgc ggggtcgaac
     1381 ccttccacag cgacagtcgt cagccgaagc cgcccgagat cgtcacgccg cggggagcct
     1441 cttgcgggcg taccgggatg ccgtgctcgt gcaggtactg cttggcctgc gagatcgtgt
     1501 agaggccgaa gtggaagatg gaagccgcga gggccgcgtc ggcgccgccc tggtcgacgg
     1561 cgagaccctc acgaagatgc tcgagctgac cgacgccgcc gctggcgatc accgggatcg
     1621 gcaggcgatg gctgacctcg cgggtgatcc acaggtcgta gccgtcgcgg gtgccgtcct
     1681 tgtccatgct tgtcagcagg acctcgccgg ccccgagcgc cgcgacgtgg tcgcaccagc
     1741 gcagcgcctc caggccggtc gac
//
LOCUS       DASWNJ010000803         2855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_650737, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000803 DASWNJ010000000
VERSION     DASWNJ010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650737"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VGB85_13565"
     CDS             <1..545
                     /locus_tag="VGB85_13565"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEY0135109.1"
                     /translation="IDRPTILSALDDLLRTELTISPGDHPQIQREPRRLHVLQWELKR
                     ACLFAVMRGVATGPRAVFILVHLLGYSPVDVATMLGVTPNSVRIHMTRATKILNDYLG
                     ARCEHLAPGNTCRCDTRLGIALERGFVRWPEHGDEMPQGPAFTGAHPDAGSLYRSLPG
                     FGLDPGATEILAAARRVSPG"
     gene            complement(588..2309)
                     /locus_tag="VGB85_13570"
     CDS             complement(588..2309)
                     /locus_tag="VGB85_13570"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0135110.1"
                     /translation="MPRRLAQIAALLTVTAGCLKIPTGEAGTAAPAATAPVAAKTLAW
                     PGKPPGQLYTVGEVRVFELIQGGKKIGTSWGRYDGPLAGDLHRFSTRVELQMPASAPN
                     TGPAGEPLRSAGEIVVDARGDLVRGYERSRAVELRFERRGDLLVFTSGREREELAFKP
                     GTAYMSFATMLHEELMFGLRRLEEGELAWRLVSLSGSLPTDWTAKLTIPAAQAPTTAV
                     VTTSLGETIHLHDGRIERIEVTSDDLEVIVPAKPPAWPTWAIDPPPTLAYAVPANATF
                     TRRELDLPGQPGEPRLAGELLIPAGKGPFPAVLFLSSTGQQDRYGFAGPPPVDLGSHV
                     ITDALAQAGIAVLRFDDRGFGDSADGPISYTGQLEDARRGLRTLLVQDEVDPDRIAII
                     GHGEGGWKALQLAGEGRNVRAVALLASPGRPYEALLKAQAEAAIQAVPPELRGEAQKT
                     QARTLAALRTGKDVPPELARTATWIREVLAIDPEALIERAEGALWIAQGEKDFEVDPG
                     KDASALARLARKYKKKHTVQLYPQLDHLFKVEPGQSSPARYLAADRAIDPKFLKDLTT
                     WLSGQLK"
     gene            2333..>2855
                     /locus_tag="VGB85_13575"
     CDS             2333..>2855
                     /locus_tag="VGB85_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEY0135111.1"
                     /translation="MPWTREDMAARVARELPAGSVVNLGIGIPTLISNYLRPEQNILL
                     HSENGLLGMGPFPYEDEVDARVINAGKQTVTVIAGGSTFDSSLSFAMIRGGHVDVAVL
                     GAMQVSATGDLANWMVPGQKVAGIGGAMDLATGARKVIALMAHSEKSGHSKIVPQCTL
                     PLTALRCVNLIVTE"
BASE COUNT          397 a          944 c         1071 g          443 t
ORIGIN      
        1 ggatcgaccg gcccacgatc ctcagcgccc tcgacgacct gttgcgcact gaattgacga
       61 tcagcccagg cgatcatccg cagatccagc gcgaacctcg gcgtctgcac gtgctgcaat
      121 gggagctcaa gcgagcttgc ctgttcgcgg tgatgcgcgg ggtcgcgacg gggccaagag
      181 cggtcttcat cctggtccat ctgctcggat actcgccggt ggatgtcgcg acgatgctgg
      241 gagtgacgcc caactcggtt cgtattcata tgactcgcgc gacgaagatc ttgaacgatt
      301 acctcggggc gcgttgcgag cacctggcgc cggggaacac ctgccgctgc gatacgcggc
      361 tgggcatcgc gctcgagcgc ggctttgtcc gctggcccga acacggcgac gagatgccgc
      421 aagggccggc cttcacggga gcccatccgg atgcaggttc cttgtatcgc tctctgccgg
      481 gcttcggact tgatccaggc gcgacggaga tcctcgcggc ggcgcgtcga gtcagcccgg
      541 gatgacctcg ccaggccacg gacggcgttc atgctttcgt gcgcgcctca cttgagctgt
      601 cccgagagcc aggtcgtcag gtcctttaag aacttcgggt cgatcgcgcg atcggccgcg
      661 aggtagcggg ccggtgagga ctgcccgggc tcgactttaa ataagtggtc gagctgggga
      721 tacagctgca cggtgtgctt cttcttgtat ttgcgggcca ggcgggccag cgcggaggcg
      781 tccttgccgg ggtcgacctc gaagtccttc tcgccctgcg cgatccacag ggcgccctcg
      841 gccctctcga tcagcgcctc ggggtcgatc gccaggacct cgcggatcca cgtcgcggtg
      901 cgggcgagct cgggcgggac gtccttgccg gtgcgcaggg cggccagcgt ccgcgcctgg
      961 gtcttttgcg cctcgccgcg cagctcgggc gggaccgcct ggatcgcggc ctcggcctgg
     1021 gccttgagca gggcctcgta ggggcggccg gggctggcga gcaacgcgac ggcgcggacg
     1081 ttgcggccct cgccggccag ctgcagggcc ttccagccgc cctcgccgtg gccgatgata
     1141 gcgatgcggt cggggtcgac ctcgtcctgc acgagcaagg tccgcaggcc gcggcgggca
     1201 tcctcgagct ggccggtgta gctgatcgga ccgtcggcgc tgtcgccgaa gccgcggtcg
     1261 tcgaagcgca gcacggcgat gccggcctgc gcgagcgcgt cggtgatcac atggctgccg
     1321 aggtcgaccg gcggcgggcc ggcgaagccg tagcgatcct gctggccggt gctcgacagg
     1381 aacagcaccg ccgggaacgg gcccttgccg gcggggatca gcagctcgcc ggcgagcctg
     1441 ggctcgccgg gctgaccggg caggtcgagc tcgcggcgcg tgaaggtggc gttcgcgggc
     1501 acggcgtagg cgagcgtcgg cggcgggtcg atcgcccacg tcggccacgc ggggggcttt
     1561 gcgggcacga tgacctcgag gtcgtcggag gtgacctcga tgcgctcgat gcggccgtcg
     1621 tgcaggtgga tcgtctcgcc gaggctcgtg gtcacgaccg cggtggtcgg ggcctgggcc
     1681 gcggggatcg tcagcttcgc ggtccagtcg gtcggcagcg agcccgacag gctgacgagg
     1741 cgccacgcca gctcgccctc ctcgagccgg cgcaggccga acatcagctc ctcgtggagc
     1801 atcgtcgcaa aggacatgta ggcggtgccg ggtttgaagg ccagctcctc gcgctcgcgg
     1861 ccggaggtga agaccagcag gtcgcccctg cgttcgaagc ggagctcgac ggcgcggctg
     1921 cgttcgtacc cgcggaccag gtcgccgcgc gcgtcgacga cgatctcgcc ggcgctgcgc
     1981 agaggctcgc cggcggggcc ggtgttcggg gcgctggcgg gcatctggag ctcgacgcgg
     2041 gtgctgaagc ggtgcaggtc gccggcgagg gggccgtcgt agcgacccca cgacgtgccg
     2101 atcttttttc cgccctggat gagctcgaac acgcgcacct cgccgacggt gtagagctgg
     2161 ccgggcggtt tgccgggcca ggcgagggtt ttggcggcca cgggggcggt cgcggccggc
     2221 gcggcggtcc cggcctcgcc ggtcgggatc ttgaggcagc cggcggtgac ggtcaggagc
     2281 gcagcaattt gggcgaggcg gcggggcacg gcgcgcacta taacctgggc ctatgccgtg
     2341 gacccgtgaa gacatggccg cccgggtggc gcgcgagctg ccggcgggca gcgtcgtcaa
     2401 cctcgggatc gggatcccga ccctgatcag caactacctg cgacccgagc agaacatcct
     2461 gctgcactcg gagaacggcc tgctcggcat ggggccgttc ccctacgagg acgaggtcga
     2521 cgcgcgggtg atcaacgccg gcaagcagac cgtcacggtg atcgccggcg gctcgacctt
     2581 cgactcgtcg ctgagcttcg cgatgatccg cggcgggcac gtcgatgtcg cggtgctcgg
     2641 ggcgatgcag gtctcggcga ccggcgacct cgccaactgg atggtcccgg gccaaaaggt
     2701 cgccggcatc ggcggcgcga tggacctcgc caccggggcg cgcaaggtga tcgcgctgat
     2761 ggcccacagc gaaaagagcg gccacagcaa gatcgtgccg cagtgcacgc tgccgctcac
     2821 ggccctgcgc tgcgtcaacc tgatcgtcac cgagc
//
LOCUS       DASWNJ010000804         2193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_15407, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000804 DASWNJ010000000
VERSION     DASWNJ010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15407"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1756)
                     /locus_tag="VGB85_13580"
     CDS             complement(<1..1756)
                     /locus_tag="VGB85_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY0135112.1"
                     /translation="MDPSTPANGPDPGYVPLWVKTNFSFLEGASHPEELVLQARAHGL
                     RALAVTDRDSVSGSVRAHVQALECGLPILHGAQLSIGEPDELLGLAELGGARKAAKKT
                     RAKKDAAWNLGPGKPLADLTPGTGGPRRGAALPPGRPLIALASDRAGYGELCRLISRG
                     RLACPKGHSRVRLAELAQAGPGLIALVVDPDMLPALRESFAGRLYALVTRHQNAAELP
                     AEARLRAVAGRLGVPVVAGAEVLYHDPARRPLQDIVTCVRMGLTLGTAGTHIRGNAEH
                     DLKSIAAMRALYRDDPAALARTLEVAERCSFSLAQLRYRYPAEAVPEGSSERDWLRHL
                     TMEGARGRYDGDPPLQVRRQIERELMLIAELDYGGYFLTMWDIVQFCRRAGILCQGRG
                     SAANSAVCYCLGITAIDPVRMDLLFERFISRERAEPPDIDLDIEHERREEVIQYVYQR
                     YGRRRAAMVAMLTRYRPRSALRDVGKALGLPQTTVDRVAKLQNMYGGEIDLAMVEAAG
                     VDATTPMHKHWLKLSAELENFPRHLATPPGGFLLGHEPLDTLVPLEPAAMAGRTIVQW
                     DKHDVEDLGLFKVDLLGLG"
BASE COUNT          297 a          838 c          735 g          323 t
ORIGIN      
        1 cgccgagccc gagcaggtcg accttgaaca gcccgaggtc ctcgacgtcg tgcttgtccc
       61 actgcacgat cgtgcggccc gccatcgccg ccggctcgag cggcaccagc gtgtcgagcg
      121 gctcgtgccc gagcaggaac ccgcccgggg gggtcgcgag gtgccgcgga aaattctcga
      181 gctcggccga gagcttcaac cagtgtttgt gcatcggtgt cgtcgcgtcg accccggccg
      241 cctcgaccat cgccaggtcg atctccccgc cgtacatgtt ctgcagcttc gccacgcggt
      301 cgacggtcgt ctgcggcagc ccgagcgcct tgccgacgtc gcgcagcgcc gagcgcggac
      361 ggtagcgcgt cagcatcgcg accatcgccg cgcgccgccg gccgtagcgc tgatacacgt
      421 actgaatgac ctcctcgcgc cgctcgtgct cgatgtcgag gtcgatgtcc ggcggctcgg
      481 cgcgctctcg gctgatgaag cgttcaaaca acaggtccat gcgcaccggg tcgatcgccg
      541 tgatccccag gcagtaacag accgccgagt tcgccgcgct gccgcgcccc tggcacagga
      601 tcccggcgcg gcgacagaac tgcacgatgt cccacatcgt caggaagtag ccgccgtagt
      661 cgagctcggc gatcaacatc agctcgcgtt cgatctgccg gcggacttgc aacggcggat
      721 cgccgtcgta gcggccgcgc gcgccctcca tcgtcaggtg acgtaaccag tcacgctcgc
      781 tcgatccctc cggcaccgcc tccgcggggt agcggtagcg cagctgcgcg agcgagaagc
      841 tgcaccgctc cgcgacctcg agcgtgcgcg ccagcgcggc cgggtcgtca cgatacagcg
      901 cccgcatcgc cgcgatcgac ttgaggtcgt gctcggcgtt gccacggatg tgcgtgccgg
      961 ccgtcccgag cgtcagcccc atccgcacgc aggtgacgat gtcctgcaac ggccggcgcg
     1021 cggggtcgtg atacagcacc tccgcgccgg cgaccaccgg caccccgagg cgccccgcca
     1081 cggcccgcag gcgcgcctcc gcgggcaatt ctgcggcgtt ctggtgccgg gtcaccagcg
     1141 cgtagagccg gccggcgaac gactcgcgca gcgccggcaa catgtccggg tcgacgacca
     1201 gcgcgatcag gcccggcccc gcctgcgcca gctcggcgag ccgcacccga ctatgtccct
     1261 tgggacaggc gagccgcccc cgcgagatca gccggcacag ctcgccgtac ccggcccggt
     1321 cgctcgccag cgcgatcagg ggccgccccg gcggcaacgc ggccccgcgc cgcgggccac
     1381 ctgtccccgg ggtcaggtcg gcgagcggct tgcccggccc gaggttccac gccgcgtctt
     1441 tcttcgcccg ggtcttcttc gcggccttcc tggccccgcc gagctcggcc agccccagca
     1501 gctcgtcggg ctcgccgatg ctcagctgcg ccccgtgaag gatcggcagg ccgcactcga
     1561 gcgcctgcac gtgcgcccgc acgctgcccg agacgctgtc gcggtcggtc accgccagcg
     1621 cgcgcagccc atgcgcccgc gcctgcaaca ccagctcctc cgggtgcgag gcaccctcga
     1681 ggaacgagaa attcgtcttg acccacaacg gcacatagcc agggtccggc ccattcgccg
     1741 gggtcgacgg atccatcact cccctccctg gggttttgga gatgtggagc acgtcgctcg
     1801 ttcgcgggac gtggagcttc gtggacacgt cgcgtgcttg agatacgtgg agcttcgcgg
     1861 acacgtcgcg cgctcgagat acgcggggcg aggcttcatg ggccggggga cgggggccga
     1921 caggcagcgg gtccctgcga cgcgggtcct cccagggcac gtgctcgccg ccccacctgt
     1981 ccggaaggac atgtcccttt caccgcccct gtctctccgc tctcgactgg cggtggggcg
     2041 tctgcgcgcg agccctgggt cctcccgctg cttcccccgc cagtcggccc ctgctccccg
     2101 ggcgaacacc gtctgcgccc cgcttgtcgt cggaagccgc ggtgtcgcgc gatggactcc
     2161 gcttggcgca agaggccgcg gtgtcggacg atg
//
LOCUS       DASWNJ010000805         2769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_580284, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000805 DASWNJ010000000
VERSION     DASWNJ010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580284"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2603)
                     /locus_tag="VGB85_13585"
     CDS             complement(<1..2603)
                     /locus_tag="VGB85_13585"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0135113.1"
                     /translation="MRAPGDITGVDPGDLRTRPLAPDDLPLPPKPASAVSPIAEPALV
                     VPPRIRHFTVLQKVGQGGMGVVYSAYDNELDRKVAIKLLQPSHDGSLGAARLHREAQA
                     MARLTHPNIVRVYEVGELEGAHHLVYLVMEFVQGETLRRWWRKARRSWREVLAVMSQA
                     GRGLAAAHAAGLVHRDFKPDNVIVADDGFVRVLDFGLARSFEAPEPAPASAPQAPMML
                     SSMSLSDQLTQAGTVIGTPAYMSPEQFSSRIVDAHSDQFSFCVVFYEALYGHRPFKAQ
                     TGAELHLAIGLLQHAEPPRKTGVPARLRKIVLRGLSAAPQDRWPSMQALLDELAREPG
                     KQARLWLAGGAIAAVAAGLSLTLAPRAAVSVCQDAPAKFAGLWDDARKAEVERNLLAT
                     GVADAADIAARVQRQLDDYTARWTAMYRESCEATQLRGEQSAQLMDLQMACLHQRRTD
                     VALLVDLLARADATVMQRVVPATLSLGRLDRCADNEALTAEVPPPEDPVQAVAVAAAR
                     EKLARVKVTFDLGQYDVSRAIIEEVVREAAVLGYPPLHAEALLRQGDLVDRSGDTVTA
                     ERLWTDAYFLAGVARDDRSQAAAATNLAFLAGAERGRYSEALLWDRHAQMLLSRLGDP
                     VDLQIARLNTMGAMMERWGKYAEGQKYLERALVLAEPRADVDPINLATVLLNSGGLYL
                     GEADYAAAERQYNRSLEIFTRLFGPNNSATADCLNNIGHIALLRADLPAAQAYFERAL
                     ATYEKSVGAGHPRLAYPLVNLGAVALALRNYDAARQHLTRALVLLEATRGVDDPQIAE
                     PTGALAELAAAQADWDEAERQAQRALLRAGGAEGEDRPDHIQPLTALGEAALGRKNFT
                     RAGEHFHRALEV"
BASE COUNT          379 a          909 c         1092 g          389 t
ORIGIN      
        1 acctccaggg cgcgatggaa gtgttcgccg gcgcgggtga agttcttgcg gccgagggca
       61 gcctcgccga gggcggttaa cggctggatg tggtcggggc ggtcctcgcc ctcggccccg
      121 ccggcgcgca ggagggcgcg ctgggcctgg cgctcggcct cgtcccagtc ggcctgcgcg
      181 gcggccagct cggcgagcgc gccggtcggc tcggcgatct gcgggtcgtc gacgccgcgg
      241 gtcgcctcga gcaacacgag ggcgcgagtg aggtgctgcc gcgcggcgtc gtagttgcgc
      301 agcgcgagcg cgacggcgcc gaggttgacc agcggatagg cgagccgcgg gtgaccggcg
      361 ccgacgctct tttcatacgt ggcgagcgcg cgctcgaagt aggcctgcgc ggccgggagg
      421 tcggcccgga ggagtgcgat gtggccgatg ttgttgaggc agtcggcggt cgccgagttg
      481 ttcgggccga acagccgcgt gaagatctcg agggaccggt tgtactggcg ctcggcggcg
      541 gcgtagtcgg cctcgccgag gtacaggccg cccgagttga gcagcacggt ggcgaggttg
      601 atcgggtcga catcggcccg cggctcggcg agcacgagcg cacgctcgag gtacttctga
      661 ccctcggcgt acttgcccca ccgctccatc atcgcgccca tcgtgttgag gcgggcgatc
      721 tgcaggtcga cggggtcgcc gaggcgagac agcagcatct gcgcgtgtcg gtcccacagc
      781 agggcctcgc tgtagcggcc ccgctccgca ccggcgagga acgcgaggtt ggtcgcggcc
      841 gcggcctggg agcgatcgtc gcgcgcgacg ccggccagga agtaggcgtc ggtccacagg
      901 cgctcggcgg tgacggtgtc gccggagcgg tcgacgaggt cgccctgacg cagcagggcc
      961 tcggcgtgga gaggcgggta gccgagcacg gcggcctcgc gcacgacctc ctcgatgatc
     1021 gcgcggctga cgtcgtactg cccgaggtcg aaggtgacct tgacgcgcgc gagcttctcg
     1081 cgcgccgcgg cgacggcgac cgcctgcacc gggtcctcgg gcggcgggac ctcggcggtc
     1141 agcgcctcgt tgtcggcgca gcgatcgagc cggcccaggc tcagcgtcgc cggcacgacg
     1201 cgctgcatca ccgtggcgtc ggcgcgcgcg agcaggtcga ccagcaacgc gacgtcggtg
     1261 cggcgctggt gcaggcaggc catctgcagg tccatcagct gcgccgactg ctcgccgcgc
     1321 agctgggtgg cctcgcagct ctcgcggtac atcgcggtcc agcgcgcggt gtagtcgtcg
     1381 agctgtcgct gcacccgcgc ggcgatgtcg gcggcgtcgg cgaccccggt ggcgagcagg
     1441 ttgcgctcga cctcggcctt gcgggcgtcg tcccagaggc cggcgaactt ggcgggggcg
     1501 tcctggcaca cgctgacggc cgcacgcggg gcgagggtga ggctgaggcc ggcggcgacg
     1561 gcagcgatcg cgccaccggc gagccacagc ctggcctgct tgccgggctc acgggcgagc
     1621 tcgtcgagca gggcctgcat cgacggccag cggtcctgcg gcgccgcgga caggccccgc
     1681 aggacgatct ttcgcaggcg cgccggtacg ccggtcttgc gcggcggctc ggcgtgttgc
     1741 agcaggccga tcgccaggtg cagctcggcg cccgtctggg ccttgaacgg gcggtggccg
     1801 tacagcgcct cgtagaacac gacacagaag ctgaactgat cgctgtgggc gtcgacgatg
     1861 cgcgacgaga actgctccgg ggacatgtac gcgggcgtgc cgatgacggt gccggcctgc
     1921 gtcagctgat cgctcagcga catggagctc agcatcatcg gcgcctgcgg agcggaggcg
     1981 ggcgcgggct ccggcgcctc gaacgagcgc gcgagcccga agtcgaggac gcgcacgaag
     2041 ccgtcgtcgg cgacgatgac gttgtcaggc ttgaagtcgc ggtggacgag gcccgcggcg
     2101 tgagcggcag cgaggcccct gccggcctgc gacatcaccg cgagcacctc gcgccacgaa
     2161 cgtctggctt tacgccacca gcgacgcaag gtctcgccct gtacgaactc catcacgagg
     2221 tagacgaggt ggtgggcgcc ctcgagctcg ccgacctcgt agacgcggac gatgttgggg
     2281 tgcgtcagtc gggccatcgc ctgggcctcg cgatgcaggc gcgcggcgcc gaggctgccg
     2341 tcgtgcgagg gctgcaggag cttgatcgcg accttgcggt cgagctcgtt gtcataggcg
     2401 gagtagacga cgcccatgcc gccctggccg accttctgca agacggtgaa gtggcggatg
     2461 cgcggcggga ccaccagcgc gggctcggcg atgggcgaga cagcgctggc aggcttcggc
     2521 ggcagcggga ggtcgtcagg cgccaggggc cgcgtgcgca ggtcgcccgg gtccacgccc
     2581 gtgatgtcgc caggagcgcg catgtgtggc ccgggcgcca gagtatcaca gggccgatgt
     2641 attgaccccg gcgggcgcgg gctcgtagca tgacacgatg cgcacctggg ctgagcagtt
     2701 gtgtctcccg tcaaggagaa agtgaccacg cggtgccggt ggcgaccatc gcgttgagga
     2761 tgacgagga
//
LOCUS       DASWNJ010000806         1835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_685246, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000806 DASWNJ010000000
VERSION     DASWNJ010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685246"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="VGB85_13590"
     CDS             <1..1265
                     /locus_tag="VGB85_13590"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY0135114.1"
                     /translation="KDDEPAPQFGAAQSNVFYLNYDGVTIQYTGQEDDSSQNISQFQE
                     FAMSYQPYGDGAKRAASSQAVKADWAKYNVVITETRPGNGSYTMCVNTPTNPYGGGVL
                     GIAPLDCNDSQARNIVFAYHSANDEYPAATQATTMSQEIAHAFGLEHVKEPNDIMNPY
                     NGGGDPAFLDQCFTLDAGGGQILCNAQHNMFCQGAQNSHQELIWLFGQSAPDLSPPSV
                     NISFPADGQIFDAGSDFSITADASDDVSVTTVEIVINGQSAVVDPSPPYSQAVQSIPA
                     GTYTVHAIATDPSANATQSATVTFTVVEGAATTDPTTDPTNVTTDPSGDPTNDPQTTG
                     FGSDSDGDSATGFETETPTTGGDEVGGGDGSASEGGIPPFETGPALPPDYGQDDEGDS
                     CSVAPRPVPTASLLVLLLALVGRRRRAA"
     gene            complement(1355..>1835)
                     /locus_tag="VGB85_13595"
     CDS             complement(1355..>1835)
                     /locus_tag="VGB85_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135115.1"
                     /translation="AGQDEAELTQKIAELLEFGTRLIWVVRLKGPLRVEVHEPGQPMR
                     IVDGDGELLAPGILQNPVPVRALIDRGAAHEAALRNLLARKGYASLDAVRDEGRDEAH
                     EQAITVTRDALHAQLHARGWTLSPALAARVQTCRELPTLLRWLTRAVTSDGPEAALD"
BASE COUNT          307 a          621 c          665 g          242 t
ORIGIN      
        1 tgaaagacga cgagccggcg ccgcagttcg gggcggccca gagcaacgtc ttttatctca
       61 actacgacgg cgtcacgatc cagtacacgg gtcaggagga cgattcgtcg cagaacatca
      121 gccagttcca ggagttcgcg atgagctatc agccctacgg cgatggcgcg aagcgggcgg
      181 cgagcagcca ggcggtcaag gccgactggg cgaaatacaa cgtggtgatc acggagacgc
      241 ggccgggcaa cggcagctat acgatgtgcg tcaatacgcc gaccaacccg tacggcggcg
      301 gcgtgctggg gatcgcgccg ctggactgca atgatagtca ggcgcgcaac atcgtgttcg
      361 cgtatcacag cgccaacgat gagtacccgg cggcgacgca ggcgacgacg atgagccagg
      421 agatcgccca cgcgttcggc ctcgagcacg tcaaggagcc gaacgacatc atgaacccgt
      481 acaacggggg cggcgatccg gcgttcctcg atcagtgctt cacgctcgac gcgggcggcg
      541 ggcagatcct gtgcaatgcg cagcacaaca tgttctgcca gggggcccag aactcgcacc
      601 aggagctgat ctggctgttc ggacagagcg cgccggacct ctcgccgccg tcggtcaaca
      661 tcagcttccc cgcggacggc cagatcttcg acgccggctc ggacttctcg atcaccgcgg
      721 acgccagtga cgacgtcagc gtgaccacgg tcgagatcgt catcaacggg caatccgcgg
      781 tcgtcgatcc gagcccgccg tactcgcagg cggtccagag catcccggct ggcacgtaca
      841 cggtccacgc gatcgccacc gacccgagcg ccaacgccac gcagagcgcg acggtgacgt
      901 tcaccgtcgt cgagggcgcg gcgacgaccg acccgacgac cgacccgacc aacgtgacga
      961 ccgacccgag cggcgacccg accaacgatc cgcagaccac cggcttcggc agcgacagcg
     1021 acggcgacag cgcgactggc ttcgagaccg agactccgac caccggcggc gacgaggtcg
     1081 gcggcggcga cggctcggcc agcgagggcg ggatcccgcc gttcgagacc ggcccggcgc
     1141 tgccgccgga ttacggccag gacgacgagg gcgacagctg cagcgtcgcg ccgcgacctg
     1201 tcccgacggc cagcttgctg gtgctgctgc tcgcgctcgt cggacgccgc cgccgcgcgg
     1261 catgaggcgg catgaggcgg catgaggcgg catgacgcgg catgaggcgg catgacgcga
     1321 gctcgcgggc tcgcgggctc gcgggctcgt gggctcagtc gagcgcggct tcgggcccgt
     1381 cgctggtgac ggcgcgcgtc agccagcgca gcagcgtcgg tagctctctg caggtctgta
     1441 cgcgggcggc gagcgccggg ctgagggtcc agccgcgggc gtgaagctgg gcgtgcagcg
     1501 cgtcgcgggt gaccgtgatc gcctgttcgt gggcctcgtc gcggccctcg tcgcggacgg
     1561 cgtcgaggct ggcgtagccc ttgcgcgcga gcaggttgcg cagcgcggcc tcgtgtgcgg
     1621 cgccgcggtc gatcagggcg cgcaccggca ccgggttctg caagatgccg ggcgcgagca
     1681 gctcgccgtc gccgtcgacg atgcgcatcg gctggccggg ctcgtggacc tcgacgcgca
     1741 gggggccctt gaggcggacg acccagatca ggcgggtgcc gaactcgagc agctcggcga
     1801 tcttctgggt gagctcggcc tcgtcctggc cggcg
//
LOCUS       DASWNJ010000807         3009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_226763, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000807 DASWNJ010000000
VERSION     DASWNJ010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226763"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2129)
                     /locus_tag="VGB85_13600"
     CDS             complement(<1..2129)
                     /locus_tag="VGB85_13600"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135116.1"
                     /translation="MARHAALFLLFFLSGISGLVYESIWSRYIRQFVGSASTAQVLVL
                     SLFMGGMALGALLASRWLGKVRRPVRAYGLIEGGIGLYALAFPWLCAGAMRLCYDQIF
                     PALGGGAVVNTIKWSMAGLLILPPCVLLGMTFPLMSAGILRSDRARTGEILSFLYFTN
                     SLGAALGAMLSGFVLVGRFGLPGTLACAAALNIVIAIVALAQRGEHAPIVAEPVAVSD
                     QSRQAGLTRLLLIVAFGTGLSSFMYEIGWIRLLSMIIGSATHSFEVMLSAFVFGLAVG
                     GLWVRKKMDRFRNPEMVLGFVQIFMGIAAIATLPLYSLAVQAMGWLMAGDIRTETTWM
                     AFNALRYLLCLVIMFPATFCAGMTLPLITHVLLRRGQGEAVIGRVYGCNTLGAIIGAT
                     LAGLLLMPLIGLQRVIVVGAVVDVLLGLVLLRVEARGAAAPRGIRRTTTLAALSSALV
                     VAFGLFVVRLDPMVLSATVYRHGRTRLGDGYELISYVDGRTASVTVIRLTNEREYRVI
                     YTNGKPDASLVLNRYPPGRDESMGPDIAGDEPNQILVGLIPLLARPDATDAALIGFGS
                     GVTCHVMLASPKLERLDTIEIEPEMVAGSRNFHPVNERAYTDPRNHFWFDDAKAYFAG
                     AAQQYDIIVSEPTNPWVSGVSSLFTVEFYREVKRYLKPRGVLAQWLQGYELSNELLLT
                     VLAALDQEFSDYQILRVGDSDWVILAVA"
     gene            complement(2146..>3009)
                     /locus_tag="VGB85_13605"
     CDS             complement(2146..>3009)
                     /locus_tag="VGB85_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135117.1"
                     /translation="EGDDRGTFGGAGSPTSASVSSTGAPGTTGDSTSGPTTSSTDPTT
                     ASTTDPALPDCGDGMCGAQEYCINCPADCGTCEPICGDMKCNGAEDCKSCEEDCVCLP
                     YCGDMACNGTENCFNCTGDCGSCEVVCGDAPCAASENCMTCAGDCGACPADCGNGTCA
                     GDETCATCSLDCGVCPPSCGDKACNGGETCETCAQDCGVCPPKCGDGQCNGNETCKSC
                     KADCAAQCTPCPCKEPPDPEKFNNVCHWPPDTASCPPTAKGGYCDPNGDGSYADADWP
                     KGYTEYNDKCL"
BASE COUNT          487 a         1034 c         1053 g          435 t
ORIGIN      
        1 gcgaccgcga ggatcaccca gtcgctgtcg ccgacgcgca ggatctggta gtccgagaac
       61 tcctggtcga gcgcggcgag gaccgtcagc agcagctcgt tgctgagctc gtagccctgc
      121 agccactgcg ccagcacgcc cctgggtttg aggtagcgct tgacctcgcg gtagaactcg
      181 accgtaaaca ggctcgacac cccggacacc caggggttgg tcggctccga cacgatgatg
      241 tcgtactgct gggccgcgcc ggcgaagtag gccttggcgt cgtcgaacca aaagtgattg
      301 cgcgggtcgg tgtaggcgcg ctcgttgacc gggtgaaagt tgcgcgagcc ggcgaccatc
      361 tcgggctcga tctcgatggt gtcgaggcgc tcgagcttcg gcgaggcgag catcacgtgg
      421 caggtcacgc ccgagccgaa gccgatcagc gcggcgtcgg tcgcgtcggg ccgggccagc
      481 agcgggatca ggccgacgag gatctggttc ggctcgtcgc cggcgatgtc ggggcccatg
      541 ctctcgtcgc ggcccggcgg gtagcggttg agcaccagcg aggcgtccgg cttgccgttg
      601 gtgtagatca cgcgatactc gcgctcgttg gtcaggcgga tgaccgtgac cgaggcggtg
      661 cgcccgtcga cgtaggagat cagctcgtag ccgtcgccga ggcgcgtgcg cccgtggcga
      721 tacaccgtcg ccgacagcac catcgggtcg aggcggacga cgaacaggcc gaacgcgacc
      781 accagcgcgc tgctcagcgc cgcgagcgtg gtcgtgcgtc ggatgccccg cggcgccgcg
      841 gcgccgcggg cctcgacgcg cagcaggacc aggccgagca ggacgtcgac gaccgcgccg
      901 acgacgatca cgcgctggag gccgatcagc ggcatcagca gcaggccggc gagcgtcgcc
      961 ccgatgatcg cgccgagcgt gttacagccg tagacgcggc cgatgactgc ctcgccttga
     1021 cctctgcgca gcagcacgtg ggtgatcagc ggcagggtca tgccggcgca gaaggtcgcc
     1081 gggaacatga tgacgaggca cagcaggtag cgcagcgcgt tgaaggccat ccaggtcgtt
     1141 tcggtgcgga tgtcgccggc catcagccag cccatcgcct gcaccgcgag cgagtacagc
     1201 ggcagcgtgg cgatcgccgc gatccccatg aagatctgca cgaagccgag gaccatctcc
     1261 gggttgcgga agcggtccat ctttttgcgc acccacaggc cgccgaccgc gaggccgaac
     1321 acgaaggccg agagcatgac ctcgaacgag tgcgtcgcgc tgccgatgat catcgacagc
     1381 aggcggatcc acccgatctc gtacatgaac gacgacagcc cggtgccgaa ggcgacgatc
     1441 agcagcaggc gggtcagccc ggcctgacgg ctctggtcac tgacagccac cggctcggcg
     1501 acgatcggag cgtgctcgcc gcgctgcgcg agcgcgacga tcgcgatcac gatgttgagc
     1561 gccgcggcgc aggcgagcgt gcccggcagg ccgaagcgac cgaccaacac gaagcccgag
     1621 agcatcgccc ccagggccgc gccgaggctg ttggtaaaat acaaaaagct caggatctcg
     1681 ccggtgcgcg cccggtcgct gcgcaggatc cccgcgctca tcagcgggaa ggtcatgccg
     1741 agcagcacgc agggcggcaa gatcagcagt ccggccatcg accacttgat cgtattgacc
     1801 acggcgccgc cgccgagcgc cgggaagatc tggtcgtaac acaggcgcat cgccccggcg
     1861 cacagccacg ggaacgccag cgcgtataga ccaataccgc cctcgatcag cccgtacgcg
     1921 cgtacgggcc ttcgaacctt gccgagccag cgcgaggcga gcagggcgcc gagcgccatg
     1981 ccgcccatga acaggctgag caccagcacc tgggccgtcg acgcgctgcc gacgaattgc
     2041 cggatgtagc gcgaccagat cgactcgtag acgaggccgc tgatgcccga gaggaagaac
     2101 aggagaaaga gggccgcgtg ccgcgccatg cggcgcgacc atacctcaga gacacttgtc
     2161 gttgtactcg gtgtagccct tgggccagtc ggcgtccgcg tagctgccgt cgccgttggg
     2221 gtcgcagtac ccgcccttgg ccgtcggcgg gcagctggcg gtgtctggcg gccagtggca
     2281 cacgttgttg aatttctccg ggtccggcgg ctccttgcac gggcacgggg tgcactgcgc
     2341 ggcgcagtcg gccttgcagc tcttgcacgt ctcgttgccg ttgcactgcc cgtcgccgca
     2401 cttgggcggg cacacgccgc agtcctgcgc gcaggtctcg caggtctcgc cgccgttgca
     2461 ggccttgtcg ccgcagctcg gcgggcacac gccgcagtcg aggctgcacg tcgcgcaggt
     2521 ctcgtcgccg gcgcaggtgc cgttgccgca gtcggccgga cacgccccgc agtcgcccgc
     2581 gcaggtcatg cagttctcgc tcgcggcgca gggcgcatcg ccgcacacga cctcgcacga
     2641 cccgcagtcg cccgtgcagt tgaagcagtt ctcggtcccg ttgcaggcca tgtcgccgca
     2701 gtacggcagg cacacgcagt cctcctcgca cgacttgcag tcctcggcgc cgttgcactt
     2761 catgtcgccg cagatcggct cgcaggtccc gcagtcggcc ggacagttga tgcagtactc
     2821 ctgcgccccg cacatgccgt cgccgcagtc gggcagggcg gggtccgtcg tgctcgcggt
     2881 cgtcgggtcg gtcgagctcg tcgtcggtcc gctcgtcgag tcgccggtgg tgccgggcgc
     2941 gccggtgctc gacaccgacg cgctggtcgg cgagccggcg ccaccaaagg tcccacggtc
     3001 gtcgccctc
//
LOCUS       DASWNJ010000808        16306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_611709, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000808 DASWNJ010000000
VERSION     DASWNJ010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 16306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16306
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611709"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..805)
                     /locus_tag="VGB85_13610"
     CDS             complement(152..805)
                     /locus_tag="VGB85_13610"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEY0135118.1"
                     /translation="MLALLLMLAQVAPVPKDSDLGAYVRDTPRCAATAKHCFGIHLHV
                     VVTPDGPAQDVAWVTAQVEQAHRHFASIDTSFEVVAADALPASELEIDSREERDALGH
                     DRFTRGVVHVFVVGRLADVDIAGNEIRGVHWRERADRTHRWVILSKIAGSLVLSHELG
                     HFFGLPHSTYDISLMNKTLRPTPLGELTFAEPEVVIMRKHRDRMLRSKMLLARKLGQ"
     gene            complement(810..1724)
                     /gene="metF"
                     /locus_tag="VGB85_13615"
     CDS             complement(810..1724)
                     /gene="metF"
                     /locus_tag="VGB85_13615"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEY0135119.1"
                     /translation="MRIRERLAHKRPVFSFEFFPPKTEKGEEVLWRSLEQLAPLGPDF
                     VSVTYGAGGSTRARTIELVGKIKQRLGVEPMAHLTCVGATRDELARVLDQLAEHDVFN
                     ILALRGDPPQGQERFEVAAGGLRYASELVAFIRERGPFCVGAACYPEVHPEAPDAEAD
                     LRNLKAKVDAGADFLISQLFFDNASFHAFERRAVEAGITIPIIPGIMPVTNFEQIRRF
                     TQMCGATLPAPLQRRLESVADDPQEVFWAGVSYAAHQCRMLLQPPVADPFSHPRPGVP
                     GIHFYTLNRSPATRAIFEILQLSRACYG"
     gene            complement(1780..4383)
                     /locus_tag="VGB85_13620"
     CDS             complement(1780..4383)
                     /locus_tag="VGB85_13620"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0135120.1"
                     /translation="MAHEGPEDEQESNKGRTVFGIGLPASVQAATKSGPAPMPRAPAR
                     APAKAPAKASAKPAPDPLAAQSRPGHAPTEDDLRAPAGHDDSAHTLHALPSQLIGAVT
                     GRTLPPARSPERGDDKELRRPQTGDYSATTPDLSQRSARVASQPGEAEGGPSFEKAHT
                     RLVAGKPIPGTRYRLLRWLGEGGMGVVYEAEHIDIERRVALKILRAELSERSDTAQVF
                     REEARAAARAGSKNNVEIFDFGELPDGRLFFCMELLNGIDLSTLTENPLDPGRTFAIL
                     RQICKGLGAAHKAGVIHRDIKPENIILVQSEGRRDMVKIVDFGVAAILDARREAGRTI
                     AGTPQYMPPEQISGADFDARLDIYAVGCVAYEMLVQHPPFTMENLADLLLAQINTVPP
                     RPSAVRPDAGPKALEEVVMRCLEKDPSKRYENMADLEASLCEAQIAAGVISEWDDLPL
                     PDVDPDRRANLLARMPSLHAEPPRRRWVGPVIAAVTAATLTGLALLILGSPEVPPEVA
                     EQIDTLSAEAREAAAVGHYVLAPEKEEAVAIKKVYALEGIEGPGEALADKTAGVLRKE
                     FSTQLLTVADKFWNQDATRPLAYDYYDWVLWFDPDNALALERTVRTAKERTDMLDRTI
                     HDQLRESDLAGMTIASAAVATGEKRNKWARQLAMNRMPLSKSIEAQEAMRGAGLAPPE
                     PEAAPAPKVDEKPATPPPPEPDPDAIIEVPDDPEPTPTEAGKKAGSKGKKRGDADADP
                     ADGEVKRDPAKASELASQGSAALSAGRRAEAEALFNQAISFDRRNGAALIGLSDIYFD
                     RGSSTKAQKYAEQAVAVAPTNSSYRIKLGDAYFNALRYRDALTHYEKAQQLGDAKAGP
                     RIARVKSKLGE"
     gene            4637..5959
                     /locus_tag="VGB85_13625"
     CDS             4637..5959
                     /locus_tag="VGB85_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY0135121.1"
                     /translation="MVANRGEVAVRISRACDLLGITPVLAVSAADRGAAYTHGRETVC
                     LGGARGAQSYLNMQAIVQAARQTECSALHPGWGFLSENPTFAALCEAHGVTFIGPPPA
                     VMHRMGKKTPAKDAMRIAGLNLIPGSDGVLRDVDEAQAVADRVGYPVLLKAESGGGGR
                     GMRIARSSSEVAGAYADAQAESTAAFGDGRLYLERLVEGGRHIEIQIMADRYGHVVHL
                     GERDCTIQRNHQKLIEESPSPVLPEAERTRTLAAAVKAAASIGYVGAGTMEFLLDQDG
                     VLRFMEMNTRLQVEHSVSEQRSGRDLVVEQIRVAAGHRLSFRQEEVALHGHVIECRIN
                     AEDATQNFRPAPGIIKRWQVPASEHLRVDTHVTDGYEVPPHYDSLLCKVIASGVTRDQ
                     ACDRMIEALEGLVCEGVPTTVPVHLAILRSPAFRANRYDTRSIPGWPP"
     gene            5978..7708
                     /locus_tag="VGB85_13630"
     CDS             5978..7708
                     /locus_tag="VGB85_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY0135122.1"
                     /translation="MAHVPLIPIGTAREQVADDRTFQDHRAALDELEANLRLRRADVQ
                     AGWGEKYAERVHAKGKLTARERLAMLIDPGTRPLEVGTFVNDGDVFGEGLRSPAAGVV
                     TAFARVEGRWCMVIANDNTVASGSWWPRTPEKIERAQTMALRLRLPTIYLVDCSGLFL
                     PEQSKAFPGGTGAGHIFKMNSLLSASGVPQLAGVFGDCIAGGGYMPIISDRVYMTEQA
                     YMVIAGAALIKGAKSQKMTSLDIGGPEVHVHLSGCADRRVPDDETLLECLRAEVRRLP
                     SSGADFYRGDAGSVEPVHATHELRGILPRDHREGYDALQVLARLCDQSLFWELMPEAG
                     EEMVCGVGRIGGLYAGFIINRQGLVGDPEHPGGMRPGGTLYRAGIAKISAFSRACDSD
                     GIPIVWLQDISGFDIGVEAERQGLLAYGSNLIYTNSTNTVPMFTVLLRKASGAGYYAM
                     AGLPYDPVVQLSTCITRLSVMEGRTLAIATYNTKLDDDFEIVSKDPAERAAIESGMAA
                     VEKRIEADMDPYVSARQMDTDEIVELGELREYLAALVEMSYQNTGYRRVKNPRIWTLH
                     DLAVLTGGIR"
     gene            7710..8330
                     /locus_tag="VGB85_13635"
     CDS             7710..8330
                     /locus_tag="VGB85_13635"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY0135123.1"
                     /translation="MPVFSSQQDSRLHPPTIQARLVRTAEGKLRLESPTVGLYRGEPR
                     PGSLVTPESSLGQLEILGVLHHLLAPAGASGLVVAPEKPGPRLTRRPVGHGDPLVTLD
                     PEAVTGAAAVGPGAAASTSTANLVFRAPTSGRWYTRPGPGKPPLIEVGAQVQQGQAVG
                     LIEVMKTFSRLHYGGEGLPSPARIRAILVKDEEDVAAGDALFELET"
     gene            8537..9592
                     /locus_tag="VGB85_13640"
     CDS             8537..9592
                     /locus_tag="VGB85_13640"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY0135124.1"
                     /translation="MATLAGAMYLCWLMLKPFIGVLLWAAVLTMSFEPVHRRLLERTK
                     SPNLSAGLACTLVVLAIGVPAGLLTWAIIHELTPALSGVEAGITDLLNPSSPITGPAI
                     EWLDDHVDIEQVRVQASEQLGNIGTALASRTLGIVGGVLGALIQFLFIIFVMYYLFRD
                     GKQVRSALSSAIPLRHRQTYAIFIRTREVVSASVYGVLVISVIQGALGGLAFWALGLP
                     SAVLWSVAMMFLSLIPLTGAFVVWIPAAIFLAASGAWVKAILLTLWGILVIGLVDNFL
                     RPKLVGERAKLHELFIFFAVLGGLQVFGLLGIVLGPVVLAIALALFDAFRHPDSTASS
                     TMLILPTGASMTPTMLP"
     gene            complement(9686..10687)
                     /locus_tag="VGB85_13645"
     CDS             complement(9686..10687)
                     /locus_tag="VGB85_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135125.1"
                     /translation="MATSKSNSKRNTSSTGSSKASGSKASGSKKASSKKASSKKASSK
                     KASSKKASSKKAASKKTASRVKTGAKKTAKKVTSAAKKTATKAGSAVKKAAKKATKAA
                     GQAGGTAKKAAKKAVSKAKSAARQANQVGSKIEAVARKVAKRVGRAAKVVNQVAGAIE
                     SLTGGGDNEGSGGQGGQGGSKRGSKSGSKSGGSKRGGSKRGGGSSGGQGGQGGGNESS
                     SNQQGGDEDSDSEDDLAPEGPPGLGQRIGVAVDTLIDVFQGDAEKPSREGETSKDDSS
                     EEAAPEAAASEEAAPEGDASEEAAPEAAAPEEAAPAEVAAPHVEYNDEKDIMDEILD"
     gene            complement(10844..12184)
                     /locus_tag="VGB85_13650"
     CDS             complement(10844..12184)
                     /locus_tag="VGB85_13650"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0135126.1"
                     /translation="MPRKSSTPLALHEVVRIVGAEHAYLFLGSERSERLTLRSAKDAE
                     GKSLPGVGGCSMAAVERVRMSREALAASGTADGTASLDGAALPSRHLLAAPLLLGHRL
                     FGVLYLDRALARGPFSEGDVEILVAIADHLALALETAHTERLARSQEAALDANRAKST
                     FIAHMSHELRTPLTAIIGYSELLEEEFQDRGDTPYIPDVQRIQSAAKHLLSLINNMLD
                     LSKIEAGKMELYLEEFQLHEVVEEAVLAVLPLLERGHNTFVRTYPADLGVVRLDKMKV
                     RQVLLNLLSNAAKFTSRGQIELEVLREPGEGPEDGERVVLRVSDTGIGMSQEQIKRIF
                     EEYAQGSAATTRRYGGTGLGLAISRSFCQRMRGDIEVASSLGEGSIFTIHLPAHLGPE
                     DAQEQDGADPTDPDGLGEPELSRTAEVPVDDPADDETTDEAASSLRSGIFPWPV"
     gene            complement(12355..12717)
                     /locus_tag="VGB85_13655"
     CDS             complement(12355..12717)
                     /locus_tag="VGB85_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135127.1"
                     /translation="MTPPGNLRPYTHLVNLVAKRSSLLLDGGPAGSQEYMRWVRSLEQ
                     DFGVRIEVRTIMGADNRPAAIGGTISSAASVADSSTGSPTPSACAEPPAGRDMMPSDC
                     QLAFTVDGDETRCALRYC"
     gene            13049..13936
                     /locus_tag="VGB85_13660"
     CDS             13049..13936
                     /locus_tag="VGB85_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY0135128.1"
                     /translation="MPPAPRLLRIASWNVNGLRACAAKGHFLPWLKGSGASILALQEV
                     RAKAEQLGPEVGRPRRWTTHFVAAEKPGYSGVGLFSRLRPEQVETSLDAPHFDAEGRV
                     QLAHFGQLVVANVYFPNGNGQERDNSRVPYKLEFYRHLFDRLAALRAQGKRVLVLGDF
                     NTAHTEIDLARPRQNLRTSGFLPEERAELSRWLAAGYVDTFRMFHADPGHYSWWSQRS
                     GARERNIGWRIDYVLACPAAAPFVREAQIHREVHGSDHCPVSVRVHPGICDRLETPAQ
                     PSGSPGSPADEDLAPVKTG"
     gene            13970..15889
                     /locus_tag="VGB85_13665"
     CDS             13970..15889
                     /locus_tag="VGB85_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HEY0135129.1"
                     /translation="MLRPQTLASLTALLLLVGCNSPAGDGKTKTEAKKVEVKTDAKTD
                     GTAGTAEKPAEKPAVVADGAAKEFVDQVDKELRELLVASSKAEWEKNTNITDATEKAA
                     AEANEKLMEYMGKTIKAATGIKTEGLDPETARKLHLIKTAASPPPAPTDAVKRKELAE
                     LGAKMEGIYGKGKYCKDPKKPKTCKDIGELSDIMTESRDYDALLDAWTGWHSIAVELR
                     PLYSRFVELANEGAREIGYTDLGELWRSGYDMSAADFEQETERLWTQVKPLYDQLHCH
                     VRAKLVEKYGKERVPEAGLIPAHLLGNMWAQEWGNIYPLVEPYPGTTSIDVTKALKAA
                     KYDELKMVKLGESFFTSLGLDPLPQTFWERSMFIQPKDREVVCHASAWDVDLNNDLRI
                     KMCIKVNQEDLITIHHELGHNYYYHYYYKLPVLFQNGAHDGFHEAIGDALALSVTPAY
                     LKQINLITEVPSDEKGLINLQMQDALDKIGFLPFGKLIDQWRWDVFSGKTKPEDYNAA
                     WWALRAKYQGIAAPGERPDPNFFDAGAKYHVPANVPYTRYFLARILQFQFHKALCQAS
                     GHTGPLYECSIYGKTAAGDKLRTMLAMGSSKPWPEALEAVTGTRQMDAQPMIEYFEPL
                     SKWLEEQNKGRTCGW"
     gene            complement(15909..>16306)
                     /locus_tag="VGB85_13670"
     CDS             complement(15909..>16306)
                     /locus_tag="VGB85_13670"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0135130.1"
                     /translation="AYEREHLAGSRAFARLSDLRFVDYHPTAPAVRGWFRHHFERVPA
                     RIGVALAVESVQAVIRAVEHGMGPGLVPAHTIAAALAEERLVAITTRRRPLTNRVTLV
                     RMLDRVPSRLEQAFVTFVVGALARPARAT"
BASE COUNT         2430 a         5767 c         5562 g         2547 t
ORIGIN      
        1 catccaaagt tgtcaaggcc ggctcccgtg aaggaatgtc agcctcgatt tactctggcg
       61 tcagattacg taattccaat tacaaaatgt acacacaacg aacgacgagc catcgccggg
      121 cctcgcacga cgccccgggc acaagcccaa actactgccc gagcttgcgc gcgagcagca
      181 tcttcgagcg cagcatgcgg tcgcgatgct tgcgcatgat gacgacctcc ggctccgcga
      241 aggtcagctc gccgagcggc gtgggccgca gggtcttgtt catcaggctg atgtcgtagg
      301 tcgagtgtgg caggccgaag aagtggccga gctcgtgcga gagcaccagc gagccggcaa
      361 ttttagaaag aatgacccag cggtgggtgc ggtcggctcg ctcgcgccag tgcacgccgc
      421 ggatctcgtt gccggcgatg tcgacgtcgg cgaggcggcc gacgacgaac acgtgcacga
      481 caccgcgggt gaagcggtcg tggccgagcg cgtcacgctc ctcgcggctg tcgatctcca
      541 gctcgctcgc gggcagcgcg tcggcggcga ccacctcgaa gctggtgtcg atcgacgcga
      601 agtggcggtg ggcctgctcg acctgcgcgg tgacccaggc gacgtcctgc gccgggccgt
      661 ccggcgtcac gaccacgtgc aggtggatcc cgaagcagtg cttcgcggtc gcggcgcagc
      721 gcggcgtgtc acgcacgtag gcgccgaggt cgctgtcctt tgggacaggc gcgacctggg
      781 cgagcatcag gagcagcgcg agcatctgtt caaccgtagc aggcgcgcga gagctggagg
      841 atctcgaaga tcgcgcgggt cgccggggag cggttgagcg tgtagaaatg gatgcccggc
      901 acgcccggcc gcgggtgcga gaaggggtcc gcgaccggcg gctggagcag catccggcac
      961 tggtgcgcgg cgtaggagac gccggcccag aacacctcct gcgggtcgtc ggcgacgctc
     1021 tcgagccggc gctgcagcgg cgccggcagc gtcgccccgc acatctgcgt gaaccggcgg
     1081 atctgctcga agttggtcac cggcatgatg cccgggatga tcgggatcgt gatgccggcc
     1141 tcgaccgcgc ggcgctcgaa ggcgtggaag ctggcgttgt cgaagaacag ctgcgagatc
     1201 aggaagtcgg cgccggcgtc gaccttggcc ttgaggttgc gcaggtccgc ctcggcgtcc
     1261 ggcgcctccg ggtgcacctc cgggtagcag gccgccccga cgcagaacgg cccgcgctcg
     1321 cggatgaagg ccaccagctc gctcgcgtag cgcaggccgc ccgccgcgac ctcgaagcgc
     1381 tcctggccct gcggaggatc gccgcgcagc gcgaggatgt tgaacacgtc gtgctcggcg
     1441 agctggtcga gcacgcgggc gagctcgtct ctcgtggcgc cgacgcaggt gaggtgggcc
     1501 atcggctcga cgccgagtcg ctgcttgatc ttgccgacca gctcgatcgt gcgggcgcgg
     1561 gtgctgccgc cggcgccgta ggtgaccgac acgaagtccg ggccgagcgg cgcgagctgc
     1621 tcgagcgatc gccacagcac ctcctcgccc ttttcggtct tcggcgggaa gaactcgaag
     1681 ctgaagaccg gccgcttgtg cgccagtcgc tcccggatcc gcatgccttg ctgcctacca
     1741 cggggcccgg gcccggtcca gagcccgggc ccggcccagc tactccccga gcttcgactt
     1801 gacccgggcg atgcgcgggc cagccttggc gtcgccgagc tgctgggcct tctcgtagtg
     1861 cgtcagcgcg tcgcggtagc gcagcgcgtt gaagtaggcg tcgccgagct tgatgcggta
     1921 gctgctgttg gtcggggcga ccgcgaccgc ctgctcggcg tacttctgag ccttggtcga
     1981 ggacccgcgg tcgaagtaga tgtcgctcag gccgatcagc gccgcgccgt tgcgacggtc
     2041 gaagctgatc gcctggttga acagcgcctc ggcctcggcg cgacgaccgg ccgacagcgc
     2101 cgcgctgccc tgactcgcca gctcgctcgc cttcgccggg tcgcgcttga cctcgccgtc
     2161 ggccgggtcg gcgtcggcat cgccgcgctt cttgcccttg ctgccggcct tcttgcccgc
     2221 ctcggtcggc gtcggctcgg ggtcgtcggg cacctcgatg atcgcgtccg gatcgggctc
     2281 cgggggcggc ggcgtggcgg gcttctcgtc gaccttcggc gccggcgcgg cctcgggctc
     2341 gggcggcgcg aggccggcgc cacgcatcgc ctcctgggcc tcgatcgact tgctcagcgg
     2401 catgcgattc atcgccagct gcctggccca cttgttgcgc ttctcgccgg tcgcgaccgc
     2461 ggcgcttgcg atcgtcatgc cggcgaggtc cgactcgcgc agctggtcgt ggatcgttcg
     2521 gtcgagcata tcggtgcgct ccttcgcggt gcggaccgtg cgctcgagcg cgagggcgtt
     2581 gtcggggtcg aaccacaaca cccagtcgta atagtcatag gccagcgggc gcgtcgcgtc
     2641 ctggttccag aacttgtcgg cgaccgtcag cagctgcgtg ctgaactcct tgcgcagcac
     2701 gccggcggtc ttgtcggcga gcgcctcgcc cgggccctcg atgccctcga gcgcgtagac
     2761 cttcttgatc gcgacggcct cctccttctc gggggcgagc acgtagtgac cgacggcggc
     2821 cgcttcgcgg gcctcggcgc tgagcgtgtc gatctgctcg gcgacctccg gtggcacctc
     2881 gggcgagccg aggatcagca gggcgaggcc ggtcagcgtc gccgcggtga cggccgcgat
     2941 caccggcccg acccagcggc ggcgcggcgg ctcggcgtgc aggctcggca tgcgcgcgag
     3001 caggttggcg cgccggtcgg ggtcgacgtc cggcagcggc aggtcgtccc actcgctgat
     3061 cacgccggcg gcgatctgcg cctcgcacag cgaggcctcg aggtcggcca tgttctcgta
     3121 gcgcttgctc gggtccttct cgaggcagcg catcacgacc tcttcgagcg ccttggggcc
     3181 ggcgtcgggg cggaccgcgc tcggccgcgg cggcacggtg ttgatctgcg cgagcagcag
     3241 gtccgcgagg ttctccatgg tgaacggcgg gtgctggacg agcatctcgt aggcgacgca
     3301 gccgacggcg tagatgtcga ggcgcgcgtc gaagtcggcg ccagagatct gctccggggg
     3361 catgtactgc ggggtgccgg cgatcgtgcg gcccgcctcg cgacgcgcgt cgaggatcgc
     3421 ggcgacgccg aagtcgacga tcttcaccat gtcgcgccgg ccctcgctct gcacgaggat
     3481 gatgttctcc ggcttgatgt cgcggtggat gacgccggcc ttgtgcgccg cgccgaggcc
     3541 cttgcagatc tggcgaagga tcgcaaaggt gcggccgggg tcgagcgggt tctcggtcag
     3601 cgtcgacagg tcgatgccgt tgagcagctc catgcagaag aacaggcgcc cgtcgggcag
     3661 ctcgccgaag tcgaagatct cgacgttgtt cttcgagccc gcacgcgcgg ccgctcgcgc
     3721 ctcctcgcgg aacacctggg cggtgtcgct gcgctcgctg agctcggccc gcaagatctt
     3781 gagcgcgacg cggcgctcga tgtcgatgtg ctcggcctcg tagacgacgc ccatgccgcc
     3841 ctcgccgagc cagcgcagca gccgataccg cgtcccgggg atcggcttgc ccgcgaccag
     3901 gcgcgtgtgc gccttctcga agctcggacc accctccgcc tcgccaggct gactcgccac
     3961 ccgcgccgaa cgctggctca ggtccggcgt cgtcgccgag tagtcccccg tctgcggccg
     4021 ccgcagctcc ttgtcgtccc cccgctcggg cgagcgcgcc ggcggcagcg tccggccggt
     4081 cacggcgccg atgagctggc tcggcagcgc gtgcaaggtg tgcgcgctgt catcatggcc
     4141 ggcgggggcc cgcaggtcgt cctcggtcgg cgcgtgcccg ggccggctct gggcggcgag
     4201 cgggtcgggt gcaggcttgg cggacgcctt ggccggcgcc ttggccggcg cgcgggccgg
     4261 tgcgcgcggc atcggagcgg ggccactctt ggtggcggcc tgcaccgagg ccggcaggcc
     4321 gatgccgaag acggttcggc ccttgttgct ctcctgctcg tcttctgggc cttcgtgcgc
     4381 catggaaacc tctttgaccg ggcgagtata cggtagcggc gccaggcgcg ttgggcggtc
     4441 gacggccgcc tgccgcgatc ttttggcgat gccagcgggg gcgaaacgtc cccgggagcg
     4501 tttcggcctg gggaggtccg ggggcgttcg cgcgcttcgc ggcgcaatcg agcgcgctgg
     4561 tgtgaaacag tgatagcctg cgcgcgccat gactgcgagg actgccgttg cccctccacc
     4621 gctgttccgt cggctgatgg tcgccaatcg cggcgaggtg gcggtccgca tctctcgcgc
     4681 ctgcgacctg ttggggatca cgccggtgct cgccgtgtcg gccgccgacc gcggcgcggc
     4741 ctacacgcac gggcgcgaaa ccgtgtgtct cggcggggcc cgcggagccc agagttactt
     4801 gaacatgcag gcgatcgtgc aggcggcccg gcagaccgag tgcagcgcgc tgcaccccgg
     4861 ctggggcttc ctgtcggaga acccgacctt tgccgcgctg tgcgaggcgc acggtgtcac
     4921 tttcatcggc ccgccgccgg cggtcatgca tcgcatgggc aagaagacgc cggcgaaaga
     4981 cgcaatgcgg atcgcaggtc tcaacctgat ccccggcagc gacggcgtgc tgcgcgacgt
     5041 cgacgaggcg caggcggtcg ccgaccgcgt cggctatccg gtgctgctca aggccgagag
     5101 cggcggcggt ggtcgcggca tgcgcatcgc ccgctcgtcg agcgaggtcg cgggcgccta
     5161 cgccgacgcg caggccgagt cgaccgcggc gttcggcgac ggccgcctct acctcgagcg
     5221 cctggtcgag ggcggccgcc acatcgagat ccagatcatg gccgaccgct acgggcacgt
     5281 cgtgcacctc ggcgagcgcg actgcacgat ccagcgcaat caccagaagc tgatcgagga
     5341 gtcgccctcg cccgtgctgc ccgaggccga gcgcacgcgg accctcgccg ccgcggtcaa
     5401 ggctgctgcg agcatcggct acgtcggcgc cggcacgatg gagttcctgc tcgaccagga
     5461 cggcgtgctg cgcttcatgg agatgaacac ccgcctgcag gtcgagcact ccgtcagcga
     5521 gcagcgctcc ggtcgcgacc tcgtcgtcga gcagatccgg gtcgccgccg ggcaccgcct
     5581 gtcgttccgc caggaggagg tcgcgctgca cggccacgtg atcgagtgcc ggatcaacgc
     5641 cgaggacgcg acccagaatt ttcgccccgc gcccggcatc atcaagcgct ggcaggtgcc
     5701 ggcgagcgag cacctgcgcg tcgacaccca cgtcaccgac ggctacgagg tcccgccgca
     5761 ctacgacagc ctgctgtgca aggtgatcgc cagcggcgtc acccgtgacc aggcctgcga
     5821 ccgcatgatc gaggccctcg agggcctcgt ctgcgagggc gtgccgacga ccgtccccgt
     5881 acacctggcg atcttgcggt cgccggcctt tcgcgccaac cgctacgaca cccggtcgat
     5941 cccgggctgg cctccctgag aggctcggta ctccaccatg gcacacgtcc cactgatccc
     6001 gatcggcacc gcccgcgaac aggtcgccga cgaccgcacc ttccaggacc accgcgccgc
     6061 cctcgacgag ctcgaggcca acctgcgcct gcgccgggcc gacgtgcagg ccggctgggg
     6121 tgagaagtac gccgagcgcg tgcacgccaa gggcaagctg accgcccgcg agcgcctcgc
     6181 catgctgatc gaccccggca cccggccgct cgaggtcggc accttcgtca acgacggcga
     6241 cgtgttcggc gagggcctgc gctcgcccgc ggccggcgtc gtgacggcct tcgcccgcgt
     6301 cgagggtcgg tggtgcatgg tgatcgccaa cgacaacacg gtcgcgtcgg gctcgtggtg
     6361 gccgcgcacg ccggagaaga tcgagcgcgc ccagacgatg gcgctgcgcc tgcgcctgcc
     6421 gacgatctac ctcgtcgact gctcgggcct gttcctgccc gagcagtcga aggccttccc
     6481 cggaggcacc ggcgccggcc acatcttcaa gatgaacagc ttgctgtccg cctcgggcgt
     6541 gccacagctg gccggggtgt tcggcgattg catcgccggc ggcggttaca tgccgatcat
     6601 ctcggatcgc gtgtacatga ccgagcaggc gtacatggtg atcgccggcg ccgcgctgat
     6661 caaaggcgcc aagtcgcaga agatgaccag cctcgacatc ggcgggcccg aggtgcatgt
     6721 gcacctcagc ggctgcgccg accgccgcgt gcccgacgac gagacgctgc tcgaatgtct
     6781 gcgcgccgag gtccggcgcc tgccgagcag cggcgccgac ttctatcgcg gtgacgccgg
     6841 cagcgtcgag ccggtgcacg cgacccacga gctgcgcggc atcttgcccc gcgaccaccg
     6901 cgagggctac gacgcgctgc aggtgctcgc gcgcctgtgc gaccagagcc tgttctggga
     6961 gctgatgccc gaggccggcg aggagatggt gtgcggggtc ggccgcatcg gtggcctgta
     7021 cgccggcttc atcatcaatc gtcagggttt ggtgggcgac cccgagcacc cgggtggcat
     7081 gcgcccgggt ggcaccctgt accgggccgg catcgccaag atctcggcgt ttagtcgcgc
     7141 ctgcgacagc gacggcatcc cgatcgtgtg gctgcaggac atcagcggct tcgacatcgg
     7201 cgtcgaggcc gagcgccagg gcctgctcgc gtacggctcc aacctcatct acaccaacag
     7261 caccaacacc gtgccgatgt tcacggtgct gctgcgcaag gccagcggcg ccggctacta
     7321 tgcaatggcc ggcctgcctt acgaccccgt cgtccagctg agcacctgca tcacgcgcct
     7381 gagcgtgatg gagggccgga ccctggcgat cgccacctac aacaccaagc tcgacgacga
     7441 cttcgagatc gtcagcaagg acccggccga gcgcgcggcg atcgagtccg gcatggccgc
     7501 ggtcgagaag cgcatcgagg ccgacatgga cccctacgtc tcggcccgcc agatggacac
     7561 cgacgagatc gtcgagctcg gggagctgcg cgagtacctc gccgcgctcg tcgagatgag
     7621 ctatcaaaac accgggtatc gccgggtcaa gaacccgcgc atctggaccc tgcacgacct
     7681 cgccgtgctc accgggggga tccgctgata tgcccgtctt cagctcgcag caagacagcc
     7741 gcctccaccc gccgacgatc caggcccgcc tcgtgcggac tgccgagggc aagctacgcc
     7801 tcgagtcccc aacggtcggc ctctatcgcg gcgaaccccg ccccggcagc ctcgtcaccc
     7861 ccgagtcctc cctcggccag ctcgagatcc tcggcgtcct gcaccacctg ctcgcccccg
     7921 ccggcgcctc cggcctggtg gtcgcgcccg agaagcccgg accccgcctg acccgccgcc
     7981 ccgtcggcca cggcgaccca ctcgtcaccc tcgaccccga ggccgtcacc ggggccgccg
     8041 ccgtcggccc gggcgccgcc gccagcacga gcaccgccaa cctggtgttc cgcgccccga
     8101 cctccggccg ctggtacacc cgccccggac ccggcaaacc gccgctgatc gaggtcggcg
     8161 cccaggtgca gcagggccag gccgtcggcc tgatcgaggt catgaagacc tttagccgcc
     8221 tccactatgg cggcgagggc ctgccctccc cggcgcgtat cagggcgatc ctcgtcaagg
     8281 acgaggaaga cgtcgcggcc ggcgacgccc tgttcgagct cgaaacctga gagcgccctg
     8341 cgcaagctgc ggcagcgctc gccggggctc gtcccggcga tgccgtcgca tctccaggcc
     8401 gacgcagcgt gacagcaacg ccgcgatgct cccggccgat taaaaaatcg acgattccgg
     8461 cgcaaactcg cggcgagcgc ccatggaaga cgacccgaat cgagaccgac gcgaccaggc
     8521 ccgctggatc atccttttgg cgacgctggc cggtgccatg tacctgtgct ggctgatgct
     8581 caagccgttc atcggcgtgc tgttatgggc cgccgtgctg acgatgagct tcgagccggt
     8641 ccaccggcgg ctgctcgagc ggaccaagag cccgaacttg agcgccgggc tggcatgtac
     8701 cctggtggtc ctggcgatcg gggtcccggc cggcctgctg acctgggcga tcattcacga
     8761 gctgacgccg gcgctcagcg gggtcgaggc cgggatcacc gacctgctca acccgagctc
     8821 gccgatcacc ggcccggcga tcgagtggct cgacgaccac gtcgacatcg agcaggtccg
     8881 ggtccaggcc tccgagcagc tcggcaacat cggcaccgcg ctcgccagcc ggacgctcgg
     8941 gatcgtcggc ggggtgctcg gcgcgctgat ccagttcctg ttcatcatct tcgtgatgta
     9001 ctacctgttc cgcgacggta aacaggtccg cagcgccctc tccagcgcga tcccgctgcg
     9061 ccaccgccag acctacgcga tcttcatacg cacccgcgag gtcgtcagcg cctcggtgta
     9121 cggcgtcctc gtgatctcgg tgatccaggg ggccctcggc ggcctcgcct tctgggccct
     9181 cggcctgccc tccgcggtgc tgtggagcgt cgccatgatg ttcctgtcgt tgatcccgct
     9241 gaccggcgcc ttcgtggtct ggatccccgc cgcgatcttt ctggccgcct ccggcgcgtg
     9301 ggtcaaggcg atcctgctga ccctgtgggg catcctcgtc atcggcctcg tcgacaactt
     9361 cctgcggccc aagctcgtcg gcgagcgcgc caagctgcac gagctgttca tcttcttcgc
     9421 cgtgctcggc ggcctccagg tgttcgggct gctcggcatc gtcctcgggc cggtcgtcct
     9481 cgcgatcgcc ctcgccctgt tcgacgcctt ccgccacccc gacagcaccg cctcgtcgac
     9541 gatgctcatc ctgccgaccg gcgcgagcat gaccccgacg atgctgccgt gaaaccccgg
     9601 gcgccccggg accccgggtc ccgcacgcag gaacgccgcg cctccgtcac gtacgacggg
     9661 agcgcggcgt tcctgtgaac agtcctcagt cgaggatctc gtccatgatg tccttctcgt
     9721 cgttgtactc cacgtggggt gcagcgactt cggcaggagc ggcctcctcg ggggcggccg
     9781 cttcaggggc ggcctcctcg gaagcatctc cttcaggagc ggcctcctcg gaggcggccg
     9841 cttcaggagc ggcctcctcg gaggagtcgt ccttggacgt ctcgccctcg cgggagggtt
     9901 tctcggcatc gccctggaac acgtcgatga gtgtgtcgac ggcgacgccg atgcgctggc
     9961 ccagaccggg tgggccttca ggggctaggt cgtcctccga atcggagtcc tcgtcaccac
    10021 cctgctggtt ggaggaggac tcgttgccgc ccccctggcc gccctggccg ccggacgagc
    10081 cgccgccgcg cttcgagccg ccgcgcttgg agccgccgga cttcgagccg gacttcgagc
    10141 cgcgcttcga gccgccctga ccgccctgac cgcccgagcc ctcgttgtcc ccaccgccgg
    10201 tgaggctctc gatggcgccc gcgacctggt tgacgacctt ggccgcgcga ccgacccgct
    10261 tggcgacctt gcgcgccacg gcttcgatct tgctgccgac ctggttggcc tgacgcgcag
    10321 cggacttggc cttgctgacg gccttcttgg ccgccttctt ggccgtgccc ccggcctgac
    10381 cggccgcctt ggtggccttc ttggccgcct tcttgaccgc gctgccggcc ttggtggccg
    10441 tcttcttggc ggcggaggtg accttcttgg cggtcttctt cgcgccggtc ttgaccctgc
    10501 tggcggtctt cttggaggcg gccttcttgg aggaggcctt cttggaagag gccttcttgg
    10561 aggaggcctt cttggaagaa gccttcttgg aagaggcctt cttgctgccg gaagccttgc
    10621 tgccggaagc cttcgatgag ccggtcgatg aggtgtttcg cttcgagttg gatttggaag
    10681 tcgccatcgc ttctccttgc tgaggcactc gttcactgag tggccatcgc gtcttgtcgt
    10741 agtctgccac ggagcggatg ctctcgggca gatcttccga agtaatatga caacgatcgt
    10801 tggttcgcgg tgctcgtcgg acgaaggcgc tacagcgcgc agttcacacc ggccacggaa
    10861 agatgccgga gcgcagggaa ctggccgctt cgtcggtggt ctcgtcgtcc gccggatcgt
    10921 cgacggggac ctcggcggtc cgcgaaagtt ccggctcgcc gaggccgtcc gggtcggtcg
    10981 ggtcggcccc gtcctgctcc tgcgcatcct ccggaccgag gtgcgccggc aggtggatcg
    11041 tgaagatgga cccctcgccg aggctgctgg cgacctcgat gtccccgcgc atgcgctggc
    11101 aaaagctccg ggaaatcgcc agcccgagcc cggtcccccc gtagcggcgg gtcgtggcgg
    11161 cgctgccctg ggcgtactcc tcgaagatgc gcttgatctg ctcctgcgac atgccgatgc
    11221 cggtgtccga gacccgcagg accacccgct cgccgtcctc ggggccctcg cccggctcgc
    11281 gcagcacctc gagctcgatc tggccgcggc tggtgaactt ggccgcgttg ctgagcaggt
    11341 tcagcaggac ctgccggacc ttcatcttgt cgaggcggac cacccccagg tcggcggggt
    11401 aggtgcggac aaaggtgttg tggccgcgtt cgagcagggg cagcaccgcc aggacggcct
    11461 cctcgacgac ctcgtggagc tggaactcct cgagatacag ctccatcttg ccggcctcga
    11521 tcttcgagag gtccagcatg ttgttgatca gcgacagcag gtgcttggcc gccgactgga
    11581 tccgctgtac gtccgggatg tacggggtat ctccccgatc ctgaaattcc tcctcgagca
    11641 gctcgctgta gccgatgatc gccgtcagcg gggtccgcag ctcgtggctc atgtgggcga
    11701 tgaaggtgct cttggcccgg ttggcgtcga gggccgcctc ctgcgaccgg gccagccgct
    11761 cggtgtgcgc cgtctcgagc gcgagcgcca ggtggtccgc gatcgcgacc aggatctcga
    11821 catcgccctc gctaaacggg ccccgggcca gcgcccggtc gaggtagagc accccgaaca
    11881 gccgatgccc caacaataaa ggagcagcga gcaggtgccg cgacggcagg gcggccccgt
    11941 cgaggctggc cgtaccgtcc gcggtgccgc tggcggccag cgcctcccgg ctcatccgca
    12001 cccgctcgac cgccgccatg ctgcacccgc cgacccccgg gagcgacttg ccctcggcgt
    12061 ccttggccga gcgcagcgtc agccgttcgc tgcgctccga cccgaggaac aggtaggcgt
    12121 gctcggcccc gacgatccgg acgacctcgt gcagcgcgag cggcgtggac gacttgcggg
    12181 gcatgagggg tcagcttgcg ggccgtgcgc gccggtccag cgcccgcatc cttgccaatc
    12241 gacgtcgcag cctagttcaa ttgaccagtg gcgatcaact gacggcccga acacgggcat
    12301 gcccgcgcgc gtccgcgcct gctggcccgg gccgtcggcc cccatccgcc cgcctcagca
    12361 gtagcgcagg gcgcagcggg tctcgtcgcc gtcgaccgtg aaggccagct ggcagtcgct
    12421 cggcatcatg tcgcggcccg cggggggctc ggcacacgcc gacggggtcg gcgacccggt
    12481 cgacgaatcg gccacggaag cggccgaaga aatcgtcccg ccgatcgccg cgggccggtt
    12541 gtcggcgccc atgatggtgc ggacctcgat gcgcacgccg aagtcctgct cgagcgaacg
    12601 gacccagcgc atgtactcct gcgagccggc cggcccgcca tcgagcagca gactgctgcg
    12661 cttcgctacc aggttgacca ggtgagtata gggacgaaga ttgccagggg gggtcacggc
    12721 accagctcca actgcccacg cacgaaaggc agctcatgct accagacggt cgccccgcgg
    12781 cgaggttttc ccccgttccc accaggcgga caccggacac cgatttgtaa ggaagatcgc
    12841 agtctgtgca gtgggtgcaa agccatgaca cgcaagatgg ccggcgaaga ctgccgaaca
    12901 tgacatcgaa tgggacaccg atcactgtca ggcggacagt cgcggacagt cgctgcaagc
    12961 ttttccggca tgtcagacac ctcgcctccc tccctcgctg ccttgctcct ggttgacacc
    13021 acggccgcgt gaggccagct ttcgccgggt gccgcctgcc ccccgcctgc tgcggatcgc
    13081 gtcgtggaac gtcaatggtc tgcgggcctg cgccgccaag ggccactttt tgccgtggct
    13141 caagggctcg ggggccagca tcctcgcctt acaagaggtc cgggccaagg ccgagcagct
    13201 ggggccggag gtcggcagac cccggcgctg gacgacccac ttcgtcgccg ccgagaagcc
    13261 gggctacagc ggcgtcggcc tgttctcgcg gctgcggccc gagcaggtcg agacctccct
    13321 cgacgccccc cacttcgacg ccgagggtcg ggtccagctg gcgcacttcg ggcagctggt
    13381 ggtcgccaac gtctacttcc cgaacggcaa cggccaggag cgcgacaaca gccgggtgcc
    13441 gtacaagctc gagttctacc gccacctgtt cgaccggctc gccgcgctgc gtgctcaggg
    13501 caagcgggtg ctggtgctcg gcgacttcaa cacggcgcac acggagatcg acctggcgcg
    13561 gccgcggcag aacctgagga ccagcgggtt cttgccggag gagcgggccg agctgtcgcg
    13621 ctggctggcg gccggatatg tcgacacctt tcgcatgttc cacgccgacc ccgggcatta
    13681 ctcgtggtgg tcgcagaggt ctggggcccg cgagcgcaac atcggctggc gcatcgacta
    13741 cgtgctcgcc tgcccggccg cggccccgtt cgtccgcgag gcccagatcc accgcgaggt
    13801 ccacggctcc gatcattgcc cggtcagcgt ccgcgttcat ccggggatct gcgatcgcct
    13861 tgaaaccccg gcccagccgt cggggtcgcc ggggtcgccg gcggacgagg atttggcgcc
    13921 cgtgaaaacc ggctgacaag ctccgcggct tcggcgtagc ttcgtcggca tgctccgccc
    13981 gcagaccctc gcatcgctga ccgcactgct gctcctggtc ggctgtaatt cgcccgccgg
    14041 tgacggtaag acaaaaaccg aggccaaaaa ggtcgaggtc aagaccgacg ccaagaccga
    14101 tgggaccgct ggcactgcgg aaaaacccgc agagaagccc gcagtggtcg ccgacggggc
    14161 cgcgaaggag ttcgtcgacc aggtcgacaa ggagctgcgc gagctgctgg tcgcctcgag
    14221 caaggcggag tgggagaaga acaccaacat caccgatgcg accgagaagg cggcggccga
    14281 ggccaacgag aagctcatgg agtacatggg caagacgatc aaggccgcga ccggcatcaa
    14341 gaccgagggc ctcgaccccg agaccgcccg caagctccac cttattaaga ccgccgccag
    14401 cccgccgccg gccccgaccg acgccgtcaa gcgcaaggag ctcgcggagc tcggcgccaa
    14461 gatggagggc atctacggca agggcaagta ctgcaaggat cccaagaagc cgaagacctg
    14521 caaggacatc ggcgagctct cggacatcat gaccgagagc cgcgactacg acgcgctgct
    14581 cgacgcctgg accggctggc acagcatcgc cgtcgagctg cggccgctgt acagccgctt
    14641 cgtcgagctc gccaacgagg gcgcgcgcga gatcggctac accgacctcg gcgagctgtg
    14701 gcgctcgggc tacgacatga gcgcggcgga cttcgagcag gagaccgagc gcctgtggac
    14761 gcaggtcaag ccgttgtacg atcagctgca ctgtcacgtg cgcgccaagc tggtcgagaa
    14821 gtacggcaag gagcgcgtgc ccgaggccgg cctgatcccc gcgcacctgc tcggcaacat
    14881 gtgggcgcag gagtggggca acatctaccc gctcgtcgag ccgtacccgg gcacgacgag
    14941 catcgacgtc accaaggcgc tcaaggcggc caagtatgac gagctcaaga tggtgaagct
    15001 cggcgagtcg ttcttcacct cgctcggcct cgacccgctg ccgcagacct tctgggagcg
    15061 ctcgatgttc atccagccca aggaccgcga ggtggtgtgc cacgcgagcg cctgggacgt
    15121 cgacctcaac aacgacctgc ggatcaagat gtgcatcaag gtcaaccagg aggacctgat
    15181 cacgatccac cacgagctcg gccacaacta ttattatcac tactactaca agctcccggt
    15241 gctgttccaa aacggcgccc acgacggctt tcacgaggcg atcggcgacg cgctggcgct
    15301 ctcggtcacg cccgcctacc tcaagcagat caacctgatc accgaggtcc cgtccgacga
    15361 gaagggcctg atcaacctgc agatgcagga cgcgctcgac aagatcgggt tcctgccgtt
    15421 cggcaagctg atcgaccaat ggcgctggga cgtgttcagc ggcaagacca agcccgagga
    15481 ctacaacgcc gcctggtggg ccctgcgcgc caagtaccag ggcatcgcag ctccgggcga
    15541 gcggcccgac ccgaacttct tcgacgccgg cgcgaagtac cacgtgcccg ccaacgtgcc
    15601 ctacacccgc tacttcctgg cccgcatcct ccagttccag ttccacaagg cgctgtgcca
    15661 ggcgtccggc cacaccggcc cgctctacga gtgctcgatc tacggaaaga ccgccgcggg
    15721 cgacaaactg cggacgatgc tcgctatggg cagcagcaag ccgtggcccg aggccctcga
    15781 ggccgtcacc ggcacccggc agatggacgc ccagccgatg atcgagtact tcgagccgct
    15841 ctcgaagtgg ctcgaggagc agaacaaggg ccgcacctgc ggctggtgat cctttaaaaa
    15901 aggagaattc acgtcgcgcg cgcgggtcgt gccagcgcgc cgacgacgaa ggtgacaaac
    15961 gcctgctcga gccggctcgg cacccggtcg agcatgcgta ccagcgtgac gcggttggtc
    16021 aatggccgcc gccgcgtcgt gatcgccacg aggcgctcct cggcgagcgc cgccgcgatc
    16081 gtgtgcgctg gcacgaggcc ggggcccatg ccgtgctcga ccgcgcggat caccgcctgc
    16141 acgctctcga ccgcgagcgc gacgccgatc ctcgctggca cgcgctcgaa gtggtgacga
    16201 aaccacccgc gcaccgcggg cgcggtcggg tgatagtcga cgaagcgcag atccgacagc
    16261 cgcgcgaacg cccggctgcc cgccagatgc tcgcgctcgt acgccg
//
LOCUS       DASWNJ010000809         2975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_120531, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000809 DASWNJ010000000
VERSION     DASWNJ010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120531"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2133
                     /locus_tag="VGB85_13675"
     CDS             <1..2133
                     /locus_tag="VGB85_13675"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0135131.1"
                     /translation="ALVIGVTVAGAMRARADAAEVCTGAAEQLTGAWDPARAAAVRTA
                     LEGTGLAYAPAAAGHVVASLDAYAQAWAAAHTQACEATAVRREQSQELLDRRMACLGE
                     RRVALRALVGVLAAADAAVVEKAVQAVAGLPRLEPCADPSYLQARVRPPEDAGAAAAV
                     ESTREQLSQAQALALAGKLTGAGQLVEAAVTQAQALAYGPLLAQALRLRGELRVAEGR
                     YIEAEQALRQAYVTARTAGDGEQAAAAATALVGVAGASLARFEAGALWRDVATAEVAG
                     SVDPLALSDLAFKTGEMAIREGEYARAEAETARALALREQVVGPNDSSLIVALNQLGG
                     ALEKQGKFAAAGQHYRRALAIGEAALGPDHPLVAAASDNLGSILQAEGRYEDALSEHL
                     RALQLRERVFGPDHMQIAGSLNNLGIVSESLGRARESEAYFRRELAIQEAHLGKTAPA
                     VALSHTNLGAILHIHGDEDGALTHLRRALEIQEEVLSPDHPEMVFVLANLGLVARAQG
                     RLQDALADLGRALRITEKTGGPEHINAAAIHQNLGSVLQQLGRTDEALAELRRARDIR
                     EQALGPTHPDVATLRANIGSILAEQGETTEAEAELRRSLLDLEAALGPTHPDLADPLV
                     GLAELLLRSGRGKEALAPAQRALDLQRAASAAPRERAAACFVLARALLASAGDRGQAV
                     ALAREAREATQPGEDREEIDRWLARHAR"
     gene            complement(2226..>2975)
                     /locus_tag="VGB85_13680"
     CDS             complement(2226..>2975)
                     /locus_tag="VGB85_13680"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY0135132.1"
                     /translation="GGRLYCAVRKAKGGPSLARAMAELLGEVDVVERDAGYFLYMSEK
                     TARFSPDAVRARLTVEYEVVDPMLGPVPLLAVPGVFCRKHLDDGTRALLTKLEALDVL
                     GDRSPRQVLDLGAGIGPLGLWAMRRWPDARLLAVESNYLAAALVRENAARLGCAPRVE
                     VLASDGLPATGAARGRVDLALVNPPTHAEPAAFAAMITPLRAWLRPGAPAWFVVNRAN
                     MLAQVLKKAGAEIEAHDVPGFTLVRATWPQV"
BASE COUNT          409 a         1115 c         1108 g          343 t
ORIGIN      
        1 gcgctggtga tcggcgtcac cgtggccggc gcgatgcgag cgcgcgccga cgcggccgag
       61 gtgtgcaccg gcgcggccga gcagctcacc ggggcgtggg atcccgcgcg cgcggccgcg
      121 gtcaggacgg cgctcgaggg caccggcctc gcctacgcgc ccgcggccgc cggtcacgtg
      181 gtcgccagcc tcgatgcata tgcgcaggcc tgggccgccg cgcacacgca ggcctgcgag
      241 gcgaccgcgg tgcgccgcga acagtcgcag gagctgctcg accgccgcat ggcctgcctc
      301 ggcgagcgtc gcgtcgccct gcgcgcgctc gtcggcgtgc tcgcggccgc cgacgcggcg
      361 gtcgtcgaga aggcggtgca ggcggtcgcc gggctgccga ggttggagcc gtgcgccgac
      421 ccgtcgtacc tgcaagcccg cgtgcgcccg ccggaggacg cgggcgcggc cgcggcggtc
      481 gagtcgacgc gcgagcagct gtcgcaggcg caggcgctcg cgctcgccgg caagctgacc
      541 ggcgcggggc agctggtcga ggccgcggtg acgcaggcgc aggcgctcgc ctacgggccg
      601 ctgctggcgc aggcgctgcg cctgcgcggt gaactccggg tcgccgaggg ccgctacatc
      661 gaggccgagc aggcgctacg tcaggcctac gtgaccgcgc ggaccgccgg tgacggcgag
      721 caggcggccg cggccgcgac ggcgctcgtc ggggtggcgg gggcctcgct ggcgcgcttc
      781 gaggcgggcg cgctgtggcg cgacgtcgcg acggccgagg tcgccggcag cgtcgacccg
      841 ctggcgctct cggacctggc cttcaagaca ggtgagatgg cgatccgcga gggcgagtac
      901 gcgcgggcag aggccgagac ggcgcgggcg ctggcgctgc gcgagcaggt cgtcgggccg
      961 aacgacagct cgctgatcgt cgcgttgaac cagctcggcg gggcgctgga gaagcagggc
     1021 aagttcgcgg cggccggcca gcactaccgc cgcgccctgg cgatcggcga ggccgcgctg
     1081 ggccccgatc atccgctcgt cgccgccgcg tcggacaacc tcggatcgat cttgcaggcc
     1141 gagggccgct acgaggacgc gctcagcgag cacctgcgcg cgctgcagct gcgcgagcga
     1201 gtgttcggcc cggaccacat gcagatcgcc ggatcgctca acaacctcgg gatcgtcagc
     1261 gagagcctcg gccgcgcgcg cgagagcgag gcctactttc gccgcgagct ggcgatccag
     1321 gaggcgcacc tcggcaagac cgcccccgcg gtcgccctct cgcacaccaa cctcggggcc
     1381 atcttgcaca tccacggcga cgaggacggc gcgctgacgc atctgcgccg tgcgctcgag
     1441 atccaggagg aggtgctgag ccccgatcac cccgagatgg tgttcgtgct cgccaacctc
     1501 gggctggtcg cccgcgccca gggcaggctg caagacgcgc tcgccgacct cggccgcgcc
     1561 ctgcggatca ccgagaagac cggcggcccg gagcacatca acgccgcggc gatccaccag
     1621 aacctcggct cggtgctgca acagctcggc cgcaccgacg aggcgctcgc cgagctgcgc
     1681 cgcgcccggg acatccgcga gcaggcgctc ggcccgaccc accccgacgt cgcgaccctg
     1741 cgcgccaaca tcggatcgat cctcgcggag cagggcgaga cgaccgaggc cgaggccgag
     1801 ctgcgacgca gcctgctcga cctcgaggcc gccctcggac ccacccaccc cgacctcgcc
     1861 gacccgctcg tcggcctcgc cgagctgctg ctgcgcagcg gtcgcggcaa agaagccctc
     1921 gcccccgccc agcgcgcgct cgacctgcaa cgcgccgcct cggccgcccc ccgcgagcgc
     1981 gccgcggcct gcttcgtgct cgcgcgggcg ctgctcgcca gcgccggcga ccgcgggcaa
     2041 gcggtcgcgc tggcccgcga ggcgcgtgag gcgacccagc ccggcgagga ccgcgaggag
     2101 atcgaccgct ggctggctcg ccacgcgcgc tgagggcggg ccgcaagaag gtttttcgag
     2161 attcttcgcg tcctgaaagg tttttccggc cgaaaaaacg ctttccaggg cgcaagctct
     2221 cgcggctaga cctgcggcca ggtcgcgcgc accagcgtga agccgggcac gtcgtgcgct
     2281 tcgatctccg cgccggcctt tttcaggacc tgcgcgagca tgttggcgcg gttgaccacg
     2341 aaccacgccg gggcgcccgg tcgcagccac gcgcgcagcg gggtgatcat cgcggcgaag
     2401 gccgccggct cggcgtgggt cggaggattg accagcgcga ggtcgacgcg gccgcgcgct
     2461 gcacccgtcg cgggcaggcc gtcgctggcg agcacctcga cgcgcggggc gcagccgagg
     2521 cgcgccgcgt tctcgcggac cagcgcggcg gcgagatagt tgctctcgac cgcgagcagc
     2581 cgcgcgtccg gccagcggcg catcgcccac aacccgagcg gcccgatgcc ggcgccgagg
     2641 tcgagcacct gccgcggcga cctgtccccg aggacatcca gcgcctccaa cttggtcaac
     2701 agcgcgcgcg tgccgtcgtc gaggtgcttg cggcagaaca cgcccggcac ggcgagcaag
     2761 ggcaccggac cgagcatggg atcgacgacc tcgtactcga ccgtcagccg ggcccggacc
     2821 gcgtcgggcg agaacctggc tgtcttttcg gacatgtaca aaaaatatcc ggcgtcgcgc
     2881 tcgacgacgt cgacctcgcc gagcagctcg gccatcgcgc gggcgagcga gggtccaccc
     2941 ttggccttgc gcacggcgca gtacaggcgt ccgcc
//
LOCUS       DASWNJ010000810         2205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_685285, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000810 DASWNJ010000000
VERSION     DASWNJ010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685285"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VGB85_13685"
     CDS             <1..668
                     /locus_tag="VGB85_13685"
                     /inference="COORDINATES: protein motif:HMM:NF014607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecO C-terminal
                     domain-containing protein"
                     /protein_id="HEY0135133.1"
                     /translation="SADHSPVGRDLDRFAYVAYLCELTDELVLARHADPLIFAALCTA
                     LEAVIAGAPRPGELRRYELALLHALGLLPALEHCCVCGAPAIASSQGVAFDAVRGGVL
                     CLSHDRGASHLPPEVLRAAGELAAGADPGPLEAAPVETRRGLRDLTQTALRAHMRRPL
                     HSLAFFAALPRGEAIQRPGTDEAPRASEPPAGDRGEPVWADEAGGEVPDQDLDVAASD
                     PDD"
     gene            829..>2205
                     /locus_tag="VGB85_13690"
     CDS             829..>2205
                     /locus_tag="VGB85_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135134.1"
                     /translation="MVGLTGCPDRNRGISPEDAGLFFPAGAVLDPRVPETTPARWMFV
                     LNANSDLVFNAGTLVPVDLERFYAAWMRDPAVCFAVDGAACTADTDCAGTQTCEADKC
                     VTDGTERAEACMAGRPDSALGEGPWTKWPIVGDVGVDLTPEVPCRHNRLKPQVIECED
                     TFFVEQDAQVFLGNFGTALRGWVRDPEDRKRDAKLLVAVRGDPSITLVDLSDDPDDPN
                     GAPILDCGQGPDSGRYDPRRCAGGHLLRFVRNEEDGAGLGAEPANILAIDGDPRLIVT
                     HATRAQVSVIDLEGQYSLRGRTDTCKGLGDDRVCKDGIPAIVHTPTVFDLNGQLGGGW
                     GLARRPCFPGTDNVPALTITRGPDGAPVECGRSLIYAGFRTNLIAARTFISEAEPLDP
                     TALGLLLSDLQARIADLQAQIEASSDDAEKAALQAELNQLRATSERYAAWLAAPQGSS
                     FNQRCLTAG"
BASE COUNT          286 a          857 c          785 g          277 t
ORIGIN      
        1 ccagcgccga tcattcgccg gtcggccgcg acctcgaccg cttcgcctac gtcgcgtacc
       61 tgtgcgagct gaccgacgag ctggtgctcg cgcgccacgc cgacccgctg atcttcgccg
      121 cgctgtgcac cgcgctcgag gcggtgatcg ccggggcgcc gcggccgggc gagctgcggc
      181 gctacgagct ggccctgctg cacgcgctgg gcctgctgcc ggcgctcgag cactgctgcg
      241 tgtgcggcgc gccggcgatc gcgtcgagcc agggcgtcgc cttcgacgcg gtgcgtggcg
      301 gggtgctgtg cctgtcgcac gaccgcggcg cctcacactt gcccccggag gtgctgcggg
      361 ccgccgggga gctggccgcc ggggccgacc cggggcccct ggaggccgcg ccggtcgaga
      421 cgcgccgcgg cctgcgagat ctgacccaga cggccctgcg agcgcacatg cggcggccct
      481 tgcactcgtt ggcgttcttc gcggcgctgc cgcggggcga ggcgatccag cggcccggga
      541 cagacgaggc cccgcgagcg tccgaacccc cggccggcga ccgtggcgag ccggtgtggg
      601 ccgacgaggc cggcggcgag gtcccggacc aggacctcga cgtcgcggcc tccgacccgg
      661 atgactgacg ccgcgcgtgc gcgggggtgg gcgggtgtga tagcttcccg ccgcatgtca
      721 cgtccgggag gcaacgcagg ccaggggtct gcgcgacgca ggcagcccgg gcgggcccga
      781 agcgccgccc tcgcgcggac atcgaccctg gccgtcgcgc ttgcgacggt ggtcggcctg
      841 accggctgtc ccgaccgcaa ccgcggcatc tcgcccgagg acgccggtct gttcttcccg
      901 gcgggcgccg tgctcgaccc gcgggtcccg gagaccacgc cggcgcgctg gatgttcgtg
      961 ctcaacgcca acagcgacct cgtgttcaac gcgggcacgc tggtgccagt cgacctcgag
     1021 cgcttctacg ccgcgtggat gcgcgacccg gcggtctgct tcgcggtcga tggtgccgcc
     1081 tgcacggccg acaccgactg cgccggcacc cagacctgcg aggccgacaa gtgcgtcacc
     1141 gatggcaccg aacgggctga ggcgtgcatg gccggtcgcc cggacagcgc cctcggcgag
     1201 ggtccatgga cgaagtggcc gatcgtcggc gacgtcggcg tcgacctgac ccccgaggtg
     1261 ccgtgccggc acaaccggct caagccgcag gtgatcgagt gcgaggacac cttcttcgtc
     1321 gagcaggacg cccaggtgtt cctcggcaac ttcggaaccg cgctgcgcgg ctgggtccgc
     1381 gatcccgagg accgcaagcg cgacgccaag ctgctggtcg ccgtgcgcgg cgacccgagc
     1441 atcactctcg tcgacctctc ggacgacccg gacgacccga acggcgcgcc gatcctcgac
     1501 tgcgggcagg ggccggactc cggccgctac gacccgcgcc ggtgcgccgg cgggcacctg
     1561 ctgcgcttcg tccgcaacga ggaggacggc gccggcctcg gcgccgagcc cgcgaacatc
     1621 ctcgcgatcg acggcgaccc gcgcctgatc gtcacccacg ccacccgcgc gcaggtgtcc
     1681 gtcatcgacc tcgagggcca gtacagcctg cgcggccgca ccgacacctg caagggcctc
     1741 ggcgacgacc gcgtgtgcaa ggacggcatt ccggcgatcg tccacacacc gaccgtcttc
     1801 gacctcaacg gtcagctcgg cggcggctgg ggcctcgcgc gccggccgtg cttccccggc
     1861 accgacaacg tgcccgcgct gacgatcacc cgcggccccg acggcgcgcc ggtcgagtgc
     1921 ggacgctcgt tgatctacgc cggctttcgc accaacctca tcgccgcgcg caccttcatc
     1981 agcgaggccg agccgctcga cccgaccgcg ctcggcctgc tgctctccga cctccaggcc
     2041 cgcatcgccg acctccaggc ccagatcgag gcgagcagcg acgacgccga gaaggccgcg
     2101 ctgcaggccg agctcaacca gctccgggcg accagcgagc gctacgccgc ctggctcgcc
     2161 gcgccgcagg gcagctcgtt caaccagcgc tgcctgaccg ccggc
//
LOCUS       DASWNJ010000811         8120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_226774, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000811 DASWNJ010000000
VERSION     DASWNJ010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 8120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8120
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226774"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..1150
                     /locus_tag="VGB85_13695"
     CDS             41..1150
                     /locus_tag="VGB85_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135135.1"
                     /translation="MPTRPPIRRFTRFPLLGIARAACLCLVLACSPDTSDLAVATDPE
                     PATGSTGSDTPTGPTTAPTGSTDAPEPELTSTTSTTDDSTGSSSTGAPAMCGDGHLDA
                     GEACDKGMENRDDGPCTLDCATAVCGDGKVYAGVESCDLGPDNSDAYGGCGDCQLNSY
                     CGDGVQDPTEQCDAGPDNGGEGSEATVPCTVGCRWDARLVFLSSAFYDGDFGGLDGAD
                     LRCRNLAKAAGITSWPTFRAWLSDAHTGPLERFVLIPAKPYGLPTGERIADSLADLVL
                     NGPRDGIRVDELGKPLPPGLVWTNTGVAGEPHSALDHCQHWDSPAPDQFARAGLSHVA
                     KAPQDAWQTWSDQRHWTSLFLWQCDKTARLYCFEN"
     gene            complement(1197..2303)
                     /locus_tag="VGB85_13700"
     CDS             complement(1197..2303)
                     /locus_tag="VGB85_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135136.1"
                     /translation="MRRALVLVLGCACTLPEIQQEGAHVRLAADPGLELCGDGVAHMD
                     RFVAALATEFGRPPPTGDDRITYHWLREYDFDVRTICVSERGGCAVGGEVFMTHAPED
                     HELVHALTWSDGVSALFFVEGLAVAYEFQGVDEQLPGAVFYGPDPVPVALADREGGWL
                     PSYRYNLAGAFTAFLIEKFGVDAYFRVYRQLQLPDTPGLVSRVLEAELGASLDALAAE
                     FDATRRDCPPMAFQRKLLECSAPEIAWDGVAWAGYRVLDCDQADVVGPFKDDSILTYQ
                     TITLPEDAYYDLTVLGDAEVGYSQIANAITLRRCGGCEDFVEAHATAGEGVVTTKLKA
                     GTYALRSRGPASAATGIGVRIERRWPAPEDEVEE"
     gene            complement(2300..5479)
                     /locus_tag="VGB85_13705"
     CDS             complement(2300..5479)
                     /locus_tag="VGB85_13705"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0135137.1"
                     /translation="MRCLAPCLGLAAALACEPASSRCGDGAPEVGVFCFPARKAVRVG
                     HGFDPEAMVVLDLDGDGALDLAAVSPARQTLSVSWGGPDGSFVRLTSWPIDGRVAGLA
                     HGDLDGDGDVDLATALPDADAVAVLEHREGREFVVRRYATGATPRGIVALDLDGGAAE
                     LVTANLGDGSLSVLRRGIADPPTVVGSAPYGLAAGDVDGDGALDLAVTLRELDAVQVM
                     RNVGGVLVPGARHEVGAVPGAIVAGDLDADGKLDLATADELGDTVSVVFGDGAGGARE
                     TERWPVMRQPGGLAIVRGGGIPPVLGVLSPETSELLRLDPRNGSTTPSGPTGVASAIA
                     AGDLDRDGREEIVYGAREEGIVGSLVPGEGVRLDSLWRAPAVHTLVALDVGGDGSEEL
                     AVVDEKGDLAIWRGPDDFLTAEPIASGFSSIVRLVAGDLDGDGLDDLVVFGSGEEEVP
                     LAVVTLLQREGGFEPGGASTAIAGTIIRVMLGDVAGDGTLDVVATIRDDAYEERGLFW
                     LPNDGFGSLGAPVALGDWPVDELERIDLDGDAALDLVTSQLGQPNVLMFEDLGPGREP
                     REVMLEDDVYGMAAGDLDGDGTDDLLICGRDGLMRLAAPTGDPAAMTRLAEERCFDAT
                     LHDLDGDGDLDVLSRPFDDSWNTRLRLSMLRNDGAGVVTAIGHAIVAGYIADFTTLAG
                     DPSGLRLSIDAADRTEVLAISIGAALVETPRAELGARLGVRFDDLDGDGQHDWWGATR
                     TELAVAFGHDGGVGPMQHVPVAGLLEPDVTDLLAVVSADLDGDDAGEIVTLGEVDLPG
                     VPLRSLEVVRVEPPGVFRGELITRIAGTATGLRVSDLDGDRHADILVSEASEEGIQLT
                     RLRGRGDGSFEGAVSQQLALPAVYYSVSLSELDGDDRLDLVILTADGVHVARGQDGGD
                     FDAPRMLWRSGGGYQSMIFGDFNADRRTDLVTAGSTGLMLLRGRGNGTFAAPRRLLSV
                     FTFSLAAGDLDGDGVDELLTTSVSSGIGSLAIGRNLGDGNFVFRSRSIGPIANSISPG
                     ISVLDLDGDGAQEIVLQDPDGLTIRRQTP"
     gene            complement(5517..6017)
                     /locus_tag="VGB85_13710"
     CDS             complement(5517..6017)
                     /locus_tag="VGB85_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135138.1"
                     /translation="MGFFDKAKQFMGGKGMANVAITVIERQPAESATFPVGDSVLKGT
                     MVIDAQKDCTLLATKYEVWLYITNDQGESPHLVCMEKDPDPNTSYSDSCLKMPCDLKA
                     GQVITQPWMVSEVDIPGILARNGFPNANQAVGDPRVRLVVKCIADVKGSPFDPSAEVT
                     VALAPS"
     gene            complement(6108..>8120)
                     /locus_tag="VGB85_13715"
     CDS             complement(6108..>8120)
                     /locus_tag="VGB85_13715"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY0135139.1"
                     /translation="AVQLFPRPLELNPFRTVVRAPGERARVPDVAARTLSREEIATMP
                     GTQGDPLRALQSLPGVARTPGGLGLLVLRGAAPNQSLVFVGEHPVPRAFHVLSLASVV
                     PGDIIDHISFVPGNFDSRYGNATGGVVVIEPRRGRRDGIHGFAKVDLAAAGTLVEGRL
                     GKKGGSFILAAQRAYIDGVLVAAQKLLGEQDFLLPRYYDYQAMVDHPLPGGGTISARV
                     IGSGDRIRLRTDQLGDGEKRTAFDFRNDYHRADIVVRKRVGPWRFMVSPAFRYDIGRR
                     AVPLQMSEQYRRDYVTSLRAEGVRELSDRFELVFGTDAQIDAFRARSMTADPNGVPDQ
                     EPKDTRGTETWLGVYTTAHLRLGPLLLTPGVRAAGFIVGADTAFAVDPRINARLELGE
                     RWNLRVGVGAYSQPRVRHYIGDARLIPGGARVGTDNLILPAFFANFEPTVSFEPLAGT
                     LRVARSVQASATIQHDLLAGYTMEATGFFREQENGVPPISAGQVLNGSTTRNLGLELL
                     IRKPLTRRLYGWVAYTLMRSEVTIQPRGDVVGGTFPADFDQRHNLAVVASYKLPHNWQ
                     IGGRFRLVSGSPYTPVLGALQYDGSFIALSGEFNSARFPPFHQLDLRVDRRWIRQRLS
                     VTAYLDVQNVYNRANIEAFIYNYDFASAAGGVGLPIFPSVGVRIDF"
BASE COUNT         1285 a         3016 c         2688 g         1131 t
ORIGIN      
        1 tactgcgtgg aacaaccctg accgtatcaa ggagatcctc atgccaacac gaccccccat
       61 ccgccgcttc acgcgcttcc ctctcctcgg catcgcccgc gccgcatgcc tgtgtctggt
      121 cctcgcgtgt agccccgaca cgagcgacct cgccgtcgcc accgatccag agcccgccac
      181 agggtcgacc ggcagcgaca cgccgaccgg gccgacgacg gccccaaccg gctcgacgga
      241 cgcgccggaa ccggagctca ccagcaccac gagcacgacc gacgactcga ccggcagcag
      301 cagcacgggc gcgcccgcga tgtgcggcga tggtcacctc gatgccggcg aggcctgcga
      361 caaaggtatg gagaaccgcg acgacgggcc ctgcacgctc gactgcgcca ccgccgtctg
      421 cggcgacggc aaggtgtacg cgggggtcga atcgtgcgac cttggccccg ataatagtga
      481 cgcctacggt ggctgcggcg actgccagct caattcctat tgcggtgacg gcgtgcagga
      541 ccccaccgag cagtgcgacg ccggccccga caacggcggc gagggctcgg aggcgacggt
      601 gccgtgcacc gtcggctgtc gctgggacgc ccgcctggtc tttttgtcga gcgcctttta
      661 cgacggcgac ttcggcgggc tcgacggcgc cgacctgcgc tgccgcaacc tcgccaaggc
      721 cgctggaatc acgtcctggc cgacctttcg cgcctggctc tccgacgcac acacgggccc
      781 gctcgagcgc ttcgtcctga tccccgccaa gccctatgga ttaccgaccg gcgagcgcat
      841 cgccgacagc ctcgccgacc tcgtgctcaa cggcccacgc gacggcatcc gcgtcgacga
      901 gctcggcaag ccgctcccac ccggactggt gtggaccaac accggcgtcg ccggcgagcc
      961 ccacagcgcg ctcgaccact gccagcactg ggacagcccg gcccccgatc agttcgcccg
     1021 tgccgggttg agtcacgtcg ccaaggcgcc gcaggacgcg tggcaaacgt ggagcgacca
     1081 gcgccactgg accagtttat ttctctggca atgcgataaa accgcccgcc tctattgctt
     1141 cgagaattga ccaacacccc gaaccaacac cccaactcgc ccgggtccgg cgccgctcac
     1201 tcctccacct cgtcctcggg cgcgggccag cggcgctcga tccggacacc gatcccagtg
     1261 gccgcgctcg ccgggccgcg cgatcgcagc gcgtaggtac cggccttgag cttcgtcgtg
     1321 accaccccct cgccggcggt agcgtgcgcc tcgacgaagt cctcgcagcc gccgcagcgg
     1381 cgcagcgtga tcgcgttggc gatctgtgaa tagccaacct ccgcgtcgcc gagcacggtc
     1441 agatcataat atgcatcctc cggcagcgtg atcgtctgat aggtgaggat cgagtcgtcc
     1501 ttgaaggggc cgaccacgtc cgcctgatcg cagtcgagca cgcgatatcc tgcccaggcg
     1561 acgccgtccc acgcgatctc gggggcgctg cactcgagca gcttgcgttg aaacgccatc
     1621 ggcggacagt cgcggcgggt cgcgtcgaac tcggccgcga gcgcgtcgag cgacgcgccg
     1681 agctcggcct ccagcacccg cgagaccagc cccggcgtgt ccgggagctg caattggcga
     1741 tacacgcgaa agtatgcatc gacgccgaac ttctcgatca aaaacgccgt gaacgcgccc
     1801 gcgaggttgt agcgataact cggcaaccac ccgccctcgc ggtcggcgag cgcgacgggg
     1861 accgggtcgg ggccataaaa caccgcgccg gggagctgct cgtcgacgcc ctgaaactca
     1921 taggcgaccg cgaggccctc gacgaagaac agcgccgaca ccccgtcgga ccacgtgagc
     1981 gcgtggacca gctcgtggtc ctcgggcgcg tgggtcatga acacttcccc cccgactgca
     2041 cagccgccgc gctcggacac gcagatcgtc cgcacgtcga agtcgtactc gcgtaaccag
     2101 tgatacgtga tgcggtcgtc gccggtcggc ggggggcggc cgaactcggt cgccagcgcc
     2161 gcgacgaagc ggtccatgtg cgcgaccccg tcgccgcaca gctcgagccc cggatccgcc
     2221 gccaggcgca catgggcgcc ctcctgctgg atctccggca gagtacacgc gcagccgagc
     2281 acgagcacga gcgcgcgtct catggggttt gccttcggat cgtcagcccg tcggggtcct
     2341 gcagcacgat ctcctgcgcg ccgtcgccgt cgaggtcgag caccgagatg ccgggggaga
     2401 tcgagttggc gatcgggccg atcgagcgcg agcgaaacac gaagttgccg tcccccaggt
     2461 tgcgaccgat cgccaggctc ccgatccccg agctgacgct ggtcgtcagc agctcgtcga
     2521 cgccatcgcc gtcgagatcg cccgccgcga gggagaacgt gaacaccgac agcaaccgtc
     2581 gcggcgcggc gaacgtgccg ttgccgcggc cgcgcagcaa catcagcccg gtggacccgg
     2641 cagtgacgag gtcggtccgc cggtccgcgt tgaagtcccc gaagatcatg ctctggtagc
     2701 cgccgcccga gcgccacagc attcgcggcg cgtcgaagtc gccgccgtcc tggccacgcg
     2761 cgacgtggac cccgtcggcc gtcaatatga ccagatcgag gcggtcgtcg ccgtcgagct
     2821 cactgagcga tacgctgtag tatacggcgg gtagcgccag ctgctgtgat acggcgccct
     2881 cgaagctgcc gtcgccgcgt ccgcgcaggc gcgtcagctg gatcccctcc tcggacgcct
     2941 cgctgacgag gatatcggcg tgacgatcgc catcgaggtc gctcacgcgc aatccggtgg
     3001 ccgtcccggc gatgcgcgtg atcagctcgc cgcgaaacac gcccggtggc tcgacacgaa
     3061 cgacctcgag cgaacgcagc ggcacaccgg gaagatcgac ctccccgagc gtgacgatct
     3121 cgccggcgtc gtcaccgtcg agatcggcgc tgacgaccgc cagcaggtcg gtgacgtccg
     3181 gctcgagcag gccagccacc ggcacgtgct gcatcggccc cacgccgccg tcgtgtccga
     3241 aggcgaccgc cagctcggtc cgcgtcgcgc cccaccagtc gtgctggccg tcgccgtcga
     3301 ggtcgtcgaa tcggactccc aggcgggcgc cgagctccgc gcgcggcgtc tcgaccagcg
     3361 cggccccgat cgagatggcg aggacctcgg tgcgatcggc ggcgtcgatc gagaggcgca
     3421 ggccggacgg gtcaccggcg agcgtcgtga agtcggcgat gtagccggcg acgatcgcgt
     3481 ggccgatcgc cgtcacgact ccggcgccgt cgttgcgcag catcgacagg cgcagccgcg
     3541 tattccagct gtcatcgaac gggcgcgaga gcacgtcgag gtcgccgtca ccgtcgaggt
     3601 cgtgcagcgt cgcgtcgaag caccgctcct ccgcgagtcg cgtcatcgct gcgggatccc
     3661 cggtcggcgc cgcgagccgc atcaggccgt cgcggccgca gatcagaagg tcgtccgtcc
     3721 catcgccgtc gaggtcaccc gcggccatac catacacgtc atcctcgagc atgacctccc
     3781 gcggctcccg cccgggcccg aggtcctcga acatcagcac attgggctgt ccgagctgtg
     3841 acgtcacgag atcgagcgcc gcatcaccgt cgaggtcgat gcgctcgagc tcgtcgaccg
     3901 gccagtcgcc gagcgcgacc ggggccccca gcgaaccgaa gccgtcattc ggcagccaga
     3961 acagcccgcg ttcctcgtat gcatcgtccc ggatcgtcgc cacgacgtcg agcgtaccgt
     4021 cacccgcgac gtcaccgagc atgacgcgaa tgatggtccc tgcgatcgcc gtgctcgctc
     4081 cgccgggctc gaagcccccc tctcgctgga gcagcgtcac caccgcgagc ggcacctcct
     4141 cctcgcccga cccgaagacg acgagatcgt cgaggccatc gccgtcgagg tcaccggcga
     4201 cgagcctcac gatcgacgag aacccgctgg cgatcggctc ggccgtcagg aagtcgtccg
     4261 gtccgcgcca gatcgccaga tcgcccttct cgtcgacgac cgcgagctcc tcgctgccat
     4321 cgccgccgac atcgagcgcg accagcgtgt gcaccgctgg cgcccgccac aacgagtcga
     4381 ggcgcacgcc ctcgccgggc acgaggctgc cgacgatccc ctcctcgcgc gccccgtata
     4441 cgatctcctc gcggccgtcg cgatcgaggt ccccggccgc gatcgcgctc gccacacccg
     4501 tcggcccgga cggcgtcgtc gagccgttgc gcgggtcgag ccgcagcagc tcggatgtct
     4561 ccggggacag gaccccgagg acaggtggta tcccgccgcc gcgcacgatc gccagcccgc
     4621 ccggctggcg catcactggc cagcgctcgg tctcccgcgc tccgccggcg ccgtcgccga
     4681 acaccacgct gaccgtgtcg ccgagctcgt cggcggtcgc caggtcgagc ttgccgtccg
     4741 cgtcgaggtc acccgcgacg atcgcccccg gcaccgcgcc gacctcgtgt cgcgcgcccg
     4801 gcacgagcac gccgccgacg ttgcgcatca cctgcaccgc gtcgagctcg cgcagcgtga
     4861 cggcgaggtc gagcgcgccg tcgccgtcga cgtccccggc cgcgagcccg tacggcgccg
     4921 acccgacgac cgtcggagga tcggcgatcc cgcggcgcaa gacgctgagg ctgccgtcgc
     4981 cgaggttggc ggtcaccagc tcggccgccc caccgtcgag gtcgagcgcg acgatcccac
     5041 gcggcgtggc gccggtcgca tatctgcgca ccacgaattc acgaccttcg cggtgttcga
     5101 gcaccgcgac cgcgtccgcg tccggcagcg cggtcgccag gtcgacatca ccgtctccgt
     5161 cgaggtcgcc gtgcgcgagc ccggcgacac gcccgtcgat cggccacgag gtcaggcgca
     5221 cgaagctgcc gtccgggccg ccccagctga ccgacagcgt ctgcctggcc gggctgaccg
     5281 ccgcgaggtc gagcgccccg tcgccgtcga ggtcgagcac caccatcgcc tcggggtcga
     5341 agccgtggcc gacgcgcacg gccttccggg ccgggaagca gaacacgccg acctcgggcg
     5401 ccccgtcgcc gcagcgcgac gacgccggct cacaagcgag cgcggcggcg aggcccaggc
     5461 acggcgcgag acaccgcatc gggcgagtgt agcccgcttt tgcgggccgt gctgcctcag
     5521 ctcggcgcga gcgcgaccgt gacctcggcg ctggggtcga agggcgagcc cttcacgtcg
     5581 gcgatgcact tgaccacgag tcgcacgcgc gggtcaccga ccgcctggtt ggcgttggga
     5641 aagccgttgc gcgcgaggat gccggggatg tcgacctcgc tgaccatcca cggctgcgtg
     5701 atcacctgcc cggccttgag gtcgcacggc atcttgaggc agctgtcgga gtagctggtg
     5761 ttggggtccg ggtccttctc catgcaaacc aggtgcggcg actccccctg gtcgttcgtg
     5821 atgtacagcc atacctcgta cttggtcgcc agcaacgtgc agtccttttg cgcgtcgatc
     5881 accatcgtgc cctttagcac cgagtcaccg accgggaacg tggccgactc ggccggctgg
     5941 cgctcgatca ccgtgatcgc cacgttggcc atgcccttgc cgcccatgaa ctgtttcgcc
     6001 ttgtcgaaga atcccatgac ggcgagctat accgcgccag ccgtcgcgac gccagccccc
     6061 tcactgcggc gcggtactgc cacggacgcc ggcgcgggcg tgcgcgctca aaaatcgatg
     6121 cgcacgccga ccgaggggaa gatcggcaac ccgaccccgc cggccgcgct ggcgaagtcg
     6181 taattataaa taaacgcctc gatgttggcc cgattataga cattctggac gtcgaggtac
     6241 gcggtgacgc tcaggcgctg gcggatccac cggcggtcga cccgcaggtc gagctgatga
     6301 aacggcggga agcgggcgct gttgaactca ccgctgagcg cgatgaagct gccgtcatat
     6361 tgcaacgcgc cgagcaccgg cgtgtacggc gagcccgaga ccaggcgaaa ccggccgccg
     6421 atctgccagt tgtgcggcag cttgtagctg gcgacgaccg cgaggttgtg gcgctgatcg
     6481 aagtccgcag gaaaggtccc gcccacgacg tcgccccggg gctggatcgt cacctcgctg
     6541 cgcatcaacg tgtacgcgac ccacccgtac agccggcgcg tcagcggctt gcggatcagc
     6601 agctcgaggc cgaggttgcg cgtcgtcgag ccgttgagca cctggccggc gctgatcggc
     6661 ggcaccccgt tctcctgctc gcgaaagaag ccggtcgcct ccatcgtgta gccggcgagc
     6721 aggtcgtgct ggatcgtcgc gctcgcctgc accgagcggg cgacccgcag cgtgcccgcg
     6781 agcggctcga agctgacggt cggctcgaag ttcgcgaaga acgccggtaa gatcaggtta
     6841 tcggtgccga cccgcgcccc gccggggatc agccgcgcgt cgccgatgta gtgccgcacg
     6901 cgcggctgcg agtacgcgcc gaccccgacg cgcaggttcc agcgctcgcc gagctcgagc
     6961 ctggcgttga tccgcgggtc gaccgcgaag gccgtgtcgg cgccgacgat gaagcccgcc
     7021 gcgcgcacgc ccggcgtcag cagcagcggg ccgagccgca gatgcgccgt cgtgtacacc
     7081 ccgagccagg tctcggtgcc gcgcgtgtcc ttgggctcct gatcaggcac gccgttcgga
     7141 tcggcggtca tcgagcgggc ccggaacgcg tctatttgcg cgtccgtgcc gaacaccagc
     7201 tcgaagcggt ccgacagctc acgtacgccc tcggcgcgca gcgaggtcac gtagtcgcgg
     7261 cgatactgct cggacatctg cagcggcacg gcgcggcggc cgatgtcgta gcgaaacgcc
     7321 gggctgacca tgaagcgcca ggggccgacg cgcttgcgca cgacgatgtc ggcgcgatga
     7381 taatcgttgc gaaagtcgaa ggccgtgcgc ttctcgccgt cgccgagttg atcggtgcgc
     7441 aggcgaatgc gatcgccgct gccgatcacg cgcgccgaga tcgttccgcc gccgggcagc
     7501 gggtggtcga ccatcgcctg ataatcgtag taacgcggca acaggaagtc ctgctcaccg
     7561 agcagcttct gcgcggcgac cagcaccccg tcgatgtagg cccgctgggc cgccaggatg
     7621 aacgacccgc ccttcttccc gagccgaccc tcgaccagcg tgccggcggc cgcgaggtcg
     7681 accttggcaa aaccatggat cccgtcgcgc cgcccgcgtc gcggctcgat caccaccacg
     7741 ccgccggtcg cgttgccgta gcgactgtcg aagttgccag gcacgaagct gatgtggtcg
     7801 atgatgtccc ccggcaccac gctcgccagc gacagcacgt gaaacgcccg cggcaccggg
     7861 tgctcaccga caaacacgag tgactggttc ggcgccgcgc cgcgcaggac cagcaggccg
     7921 agcccacccg gcgtgcgcgc gacccccggg aggctctgca aggcccgcag cgggtcgccc
     7981 tgcgtgcccg gcatcgtcgc tatctcctcg cggctgagcg tgcgcgccgc gacgtcgggc
     8041 acgcgcgccc gctcgcccgg cgcgcgcacg accgttcgaa acggattgag ctcgagcggc
     8101 cgcggaaaca gctgcaccgc
//
LOCUS       DASWNJ010000812         2464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_580376, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000812 DASWNJ010000000
VERSION     DASWNJ010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580376"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..998
                     /locus_tag="VGB85_13720"
     CDS             102..998
                     /locus_tag="VGB85_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135140.1"
                     /translation="MSTLDMGPIPDFGGQPIGCQGKIDFLFVISSWYSMKYNQTQLQE
                     AFPAFTAMLKDEFADFDYHIMVVESGGNALLNKCDACYDCTGCTKPGCAEYGGPEDYP
                     CKGPFEACDSVSGAGVTITGNFGATNKRCDLFGDNRYIIKGEPDPETMFKCIATVGEG
                     PKTPVPMEAMQAALQPDILSGGCNDGFLRKDALLAVVVLNRTQDNGSAGTPQDWYDAL
                     VAAKGGNEEAIVTLVLSNDQDLPEPKCPTNGDASAPNPLRLFAETAAHGRFESTCVES
                     YVPALKATAATIVEQCSLLIPG"
     gene            complement(1003..>2464)
                     /locus_tag="VGB85_13725"
     CDS             complement(1003..>2464)
                     /locus_tag="VGB85_13725"
                     /inference="COORDINATES: protein motif:HMM:NF028473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135141.1"
                     /translation="LPIDPPPTIADLACDPEVADEVGPTICTYTASADAVVADLLDDG
                     ELIASGPASGSVIFPVTSGPNNNPGSMITVTVRDEAGQTASASLYQASTVQEPGSEKW
                     TTIEPNDGAFSLAGAVALQGDLVLAAGVHYKNPQVIGTLRRYDQGGVWMPSDVGWSKS
                     HGDWTKLAWLKSGNFGPTGLAVDAEQNIILVGLAYHDGQPRSYVARFHPDGELDWEWA
                     GSLGTEARGVGVQPDGTIYVAGSKRTSVNPERWDMEITVLGSEKTAYGPISYSDPEDD
                     ENLRNERGRAVVVLKSGRVAIVGTREIVGPNDPNDQDNWITRGVALLFEGKGKRVGDD
                     WTSTGDKLKHDAILAGAATKDGFATCGYAHADPDEPGSKNQILIRWHGENLQEIKAPR
                     LETTQGAAVCNALGYNMEGATIVGAHVNVLGENDNQWIFAVRDATDLRVDYLKHNGAS
                     NGPDRVQGLDCEYLCAWVGVESVDGAMQWVAGLFRG"
BASE COUNT          391 a          851 c          808 g          414 t
ORIGIN      
        1 gcagctccgg cagcggggcg agcaccagca gctccggcag caccagcggc gaggacaaca
       61 gcagcgcagc ggtgagcgcg agcggtgcga ccactggcaa catgtcgacg ctggacatgg
      121 gaccgatccc cgacttcggt gggcaaccga tcggctgtca gggaaagatc gacttcctgt
      181 tcgtgatctc gtcctggtac tcgatgaagt acaaccagac gcagctccag gaggcgttcc
      241 cggccttcac agcgatgctc aaggatgagt tcgccgactt cgactaccac atcatggtcg
      301 tggaatccgg tggcaacgcg ctcctcaaca agtgcgacgc ctgctatgac tgcaccggct
      361 gcacgaagcc aggctgtgcc gaatacggcg gcccggagga ctacccctgc aaggggccgt
      421 tcgaagcatg cgactccgtg tctggcgcgg gcgtgacgat cacgggcaac ttcggcgcga
      481 cgaacaagcg ctgcgacctg ttcggcgaca accgctacat catcaagggc gagcccgatc
      541 ccgagacgat gtttaagtgc atcgccaccg tcggcgaagg ccccaagacg ccggtgccga
      601 tggaggcgat gcaggcggcg cttcagcccg acatattgag cggcggctgc aacgacggct
      661 ttctccgcaa ggacgcgctt ctcgcggtgg tggtcctcaa tcgtacgcag gacaacggca
      721 gcgcgggcac gccacaggac tggtacgacg cgctcgtcgc cgccaagggg ggcaatgagg
      781 aggcgatcgt cacgctcgtg ctgtccaacg accaggacct gccagagccc aagtgcccga
      841 cgaacggtga cgcttcagct ccgaacccgc tgcgcctgtt cgcggagacc gccgcgcacg
      901 ggcgcttcga gagcacctgc gtcgagagct acgtccccgc cctgaaagcg acggccgcga
      961 cgatcgtcga gcagtgctcg ctcctgatcc cggggtgacc tgctacccgc ggaacaggcc
     1021 ggcgacccac tgcatggcgc cgtcgaccga ctcgacgccg acccacgcac acaggtactc
     1081 gcagtcgagc ccctgcacgc ggtcgggtcc gttgctcgcg ccgttgtgtt tcaggtagtc
     1141 gacccgcaga tcggtcgcat cacgcacggc gaagatccac tggttgtcgt tctcgccgag
     1201 cacgttgacg tgcgcgccga cgatcgtcgc gccctccatg ttgtagccga gcgcgttgca
     1261 cacggcggcg ccctgtgtgg tctccaggcg cggggccttg atctcctgga ggttctcgcc
     1321 gtgccagcgg atgaggatct ggttcttgct ccccggttcg tccgggtccg catgcgcgta
     1381 gccgcaggtc gcaaagccgt ccttggtggc cgcacccgcc aggatcgcgt cgtgcttcaa
     1441 cttgtcgccg gtggacgtcc agtcgtcgcc gacgcgcttg cccttgccct cgaacagcag
     1501 cgccacaccc cgcgtgatcc aattgtcctg gtcgttcggg tcgttcggac cgacgatctc
     1561 acgggtgccg acgatggcga cgcggccgct cttgagcacg acgacggcgc ggccgcgctc
     1621 attgcgcagg ttctcatcgt cttccgggtc gctgtaggag atgggcccgt aggcggtctt
     1681 ctcgctaccg aggacggtga tctccatgtc ccaccgctcc gggttgacgc tcgtgcgctt
     1741 cgagcccgcg acgtagatcg tgccgtccgg ctggacgccc acgccgcggg cctcggtccc
     1801 gagcgaacca gcccactccc agtccagctc gccgtccggg tggaagcggg cgacgtagct
     1861 gcgtggctgg ccgtcatggt aggcgaggcc gacgaggatg atgttctgct cggcgtcgac
     1921 cgcgaggccc gtcggtccga agttcccaga cttgagccac gccagcttgg tccagtcgcc
     1981 gtgcgacttg ctccacccga cgtcgctcgg catccacacg ccgccctggt cgtagcggcg
     2041 cagggtcccg atcacctggg ggttcttgta atggacgccg gcggcgagaa cgaggtcccc
     2101 ttgcagggcg acagcccccg ccaagctaaa cgcgccgtcg tttggttcga tcgtggtcca
     2161 cttctcgctc cccggctcct ggaccgtgct cgcctggtac agcgacgcgg acgcggtctg
     2221 ccccgcctcg tcgcgcaccg tcaccgtaat catgctcccg gggttgttgt tcggcccgct
     2281 ggtgaccggg aagatcaccg agccgctcgc cgggccgctg gcgatcaatt cgccgtcgtc
     2341 caggagatcg gccaccaccg cgtccgcgct cgccgtgtag gtgcagatcg tggggccgac
     2401 ctcatccgcg acctcggggt cgcaggccag atcggcgatc gtcggcggcg gatcgatcgg
     2461 cagc
//
LOCUS       DASWNJ010000813         1625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_508129, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000813 DASWNJ010000000
VERSION     DASWNJ010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508129"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..764
                     /locus_tag="VGB85_13730"
     CDS             120..764
                     /locus_tag="VGB85_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HEY0135142.1"
                     /translation="MKVSPEAPIPDWSGLETAFEHNAPDHHAYLDLSNGQVVTINDSR
                     PEDEEKRQLISKSTGRFVHLDPASSREQYRWMERFVASVEDTSLRERLVLAIDGKGAF
                     RRFKDVLLSYPVERDRWFSYRANLLHIYIDGWLAGQDVLLGAYPPWGKPEEPTEPNVP
                     LEKPPGERGAGASETLRRTARDLIDRLPALELPAAIAYLQYLHERGPSVTEPPT"
     gene            797..>1625
                     /locus_tag="VGB85_13735"
     CDS             797..>1625
                     /locus_tag="VGB85_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135143.1"
                     /translation="MSHETGRNFSRFIGVNLGGGRGKTTAVARLERSGRGVRLVEARM
                     RHGHRGGGEDTAADLGDPLFRDEVLVGYLLRNLDDETAVAINAPLTLPPCIRCPLPCP
                     GVQACEVPVVAWMRRHAPALLTHRGRSDRGKPAVTPYTQRAADLLLAHAGLQPRESLG
                     QGMGPLAARAAYLRHALSPQLRLHENLIEVHPTATLIRLFGATTVHRLGRGPTDEVAE
                     QRRKIFVGLGEKLRFDYVWPEIVVRSPHIFEAVLSAFTAFLWATQGWEGPTDLLAPAS
                     "
BASE COUNT          236 a          602 c          563 g          224 t
ORIGIN      
        1 cgaccttccg cgccccgcct gcatttgttc cgggccacct ggcctgaaat agcgccgcgc
       61 ctgcccagcg cggccctgcg catgcggtcg ccggatccta actagcgaaa gcgactgcca
      121 tgaaagtctc ccccgaagcg ccgatccccg actggtcggg cctggaaacc gcgttcgagc
      181 acaacgctcc ggatcatcac gcgtacctcg acctgagcaa cggtcaggtc gtcacgatca
      241 acgacagccg ccccgaggac gaggagaagc gccagctcat cagcaagagc accggccgct
      301 tcgtgcacct cgatccggcg tcgtcacgcg agcagtatcg gtggatggag cgcttcgtcg
      361 cctccgtcga ggacaccagc ctgcgcgagc gcctggtgct cgcgatcgac ggcaagggcg
      421 cgtttcgccg gttcaaggac gtcctgctct cgtatccggt cgagcgcgac cggtggttca
      481 gttaccgcgc caacctgctg cacatctaca tcgacggctg gctcgccggg caggacgtcc
      541 tgctcggcgc ctacccgccg tggggcaagc cggaggagcc gaccgagccc aacgtgccgc
      601 tcgagaagcc gccgggcgag cgcggcgcgg gggcctcgga gacgctgcgc cgcaccgccc
      661 gcgacctgat cgaccgcctg ccggcgctcg agctgcccgc cgcgatcgcg tatctgcagt
      721 acctgcacga gcgcggccca tcggtgaccg agccgccgac gtgagcgcga ccggcgacgc
      781 aggtggctcc cgggacatgt cccacgagac aggccgaaac ttcagccgct tcatcggcgt
      841 gaacctcggg ggcgggcgcg gcaagaccac cgcggtcgcc cgcctcgagc gcagcggtcg
      901 cggcgtgcgg ctggtcgagg cgcgcatgcg tcacggccac cgcggcggcg gcgaggacac
      961 ggccgcggac ctcggcgacc cgctgttccg tgacgaggtc ctcgtcggct atctgctgcg
     1021 caacctcgac gacgagacgg cggtcgcgat caacgcgccg ctgaccctgc cgccgtgcat
     1081 ccgctgcccc ttgccgtgcc ccggcgtgca ggcctgcgag gtgccggtgg tcgcgtggat
     1141 gcgccgccac gcgcccgcgc tgctgaccca ccgcggtcgc agcgatcgcg gcaagccggc
     1201 ggtgacgccg tacacgcagc gcgcggccga cctgttgctc gcgcacgcgg gtctgcagcc
     1261 gcgggagtcg ctggggcagg ggatgggccc gctggcggcc cgcgcggcgt acctgcgcca
     1321 cgccctctcg ccgcagctgc gcctgcacga gaacctgatc gaggtccacc cgacggcgac
     1381 cctgatccgc ctgttcggcg cgacgaccgt gcaccgcctc gggcgcggac cgaccgacga
     1441 ggtcgccgag caacgccgca agatcttcgt cgggctcggc gagaagttac gcttcgatta
     1501 cgtgtggccg gagatcgtgg tccgaagccc ccacatcttc gaggcggtgc tgtcggcctt
     1561 cacggcgttc ctgtgggcga ctcaggggtg ggaggggccg actgatctgc tcgcgcccgc
     1621 gagcg
//
LOCUS       DASWNJ010000814         3975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_650929, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000814 DASWNJ010000000
VERSION     DASWNJ010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650929"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VGB85_13740"
     CDS             <1..545
                     /locus_tag="VGB85_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135144.1"
                     /translation="SVYKEAFTCATEDPADCDSAWAYYTGLEPMNSGKGLSRAVLDNS
                     KEAHERIWDGIRAVRCWRDLSKDEGGYPFLEMVDADSRALFEQAWEQLDQALHRGFAV
                     IVREHVEAYKQSACGEGDLYLPAAWAYLQIAGPVLQREAAERDATKAKVLADLWASDA
                     PTEQQLVDGIAALDAIFPCA"
     gene            complement(607..678)
                     /locus_tag="VGB85_13745"
     tRNA            complement(607..678)
                     /locus_tag="VGB85_13745"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(644..646),aa:Val,seq:tac)
     gene            complement(704..1294)
                     /gene="folK"
                     /locus_tag="VGB85_13750"
     CDS             complement(704..1294)
                     /gene="folK"
                     /locus_tag="VGB85_13750"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEY0135145.1"
                     /translation="MLARARRNLVTRLPHKGLADDLAAPAAVYVALGSNLGDRLATLQ
                     SVARELRDGAVPGTALVRSSPVYETRPLGPSTWAYLNAVIELRTVAEPHALLSALQDL
                     ELRHGRIRRERWEARTLDLDILVWLTAGGELECETPGLRLPHPELGRRDFVLAPLAAL
                     APDLRPGRGTRTTSELLAALAPADLTIVAQVAEQLC"
     gene            complement(1343..2194)
                     /locus_tag="VGB85_13755"
     CDS             complement(1343..2194)
                     /locus_tag="VGB85_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135146.1"
                     /translation="MLARWTCLAVAVQLAGCAQISETTRVHALAVPGPRLLGSERAVT
                     ERQLELRWVQRGDSLSVELLEHRRCQVVERVMVAREEHRTRRADAAIYWEYGLATVLL
                     GLAAFSFARPDLFAVPTPVPGGELRDPKTGYKLGGVFMGLGTVALGAGIYDSVRARDH
                     RRRSDVVELRPGPAVECDEPTLPATVRKLGLQLGEHRSQATTDLEGRARFVLPGPELW
                     PPVPPAPADEPPGMDADSGPAAPQRRRWSGALDVGLGRSVAIEIVLPYDQTMLAPSSG
                     AAMSVTR"
     gene            complement(2202..2519)
                     /gene="cyaY"
                     /locus_tag="VGB85_13760"
     CDS             complement(2202..2519)
                     /gene="cyaY"
                     /locus_tag="VGB85_13760"
                     /inference="COORDINATES: protein motif:HMM:NF013643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEY0135147.1"
                     /translation="MDVKEFHKLSAKALEHIDTALGEADLDGLDIQHAGDVLTISFAD
                     GARFVINAHSAAMQVWMAAGTTAWHFDHESDGRWVARRTGDELMQTVARVVSDKMGTR
                     VAV"
     gene            complement(2556..2876)
                     /locus_tag="VGB85_13765"
     CDS             complement(2556..2876)
                     /locus_tag="VGB85_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135148.1"
                     /translation="MTRRGLLIRLLIFVPLFAYFGYGAYQKWRSENEYAHAEAEAAAA
                     REVELKGATRSVTLHDGRSIDIVELTPEQAERLHGIKMPPAEDKKAAEAPTATPTPPA
                     EAAN"
     gene            complement(2954..3706)
                     /locus_tag="VGB85_13770"
     CDS             complement(2954..3706)
                     /locus_tag="VGB85_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135149.1"
                     /translation="MRRVLCVLGAVLVSAQGIGCGGSQACADLGDDVLLTAQVTDNGE
                     RARVEVELRRPDLEDGSIPVKLCEDHGLRVDDVDMTEVKRPSGAVIYEAELSAVSGQQ
                     ALARRFELRTDDARTEFTARIDAPGFAITSPMADSELSRAAPLTITWDPPRGGEAKIA
                     VKVADEIDGDACLGEPIELEEPDDGEVVVGASQVELTPKMPPSDGVCEAFVTLSRTHS
                     APLELVDGSGGLHPDSRVEATTSRTVAFVSVP"
     gene            complement(3759..>3975)
                     /locus_tag="VGB85_13775"
     CDS             complement(3759..>3975)
                     /locus_tag="VGB85_13775"
                     /inference="COORDINATES: protein motif:HMM:NF015608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEY0135150.1"
                     /translation="AGYLGQPWFQVKLFGLAILFSYHFYAGVVRKQLAAGTCRLTSRQ
                     CRLINEVPILPLLLIVIMAVVKPLLWG"
BASE COUNT          561 a         1478 c         1383 g          553 t
ORIGIN      
        1 tctcggtcta caaggaggcc ttcacctgcg ccaccgagga ccccgccgac tgtgactccg
       61 cctgggcgta ctacaccggc ctcgagccga tgaacagcgg caagggcctg tcacgcgcgg
      121 tgctcgacaa ctcgaaggag gcccacgagc gcatctggga cgggatccgc gcggtccgtt
      181 gctggcgcga cctctccaaa gacgagggcg gctacccctt cctcgagatg gtcgacgcgg
      241 acagcagggc gctgttcgag caggcgtggg agcagctcga ccaggcgctg caccgcggct
      301 tcgcggtgat cgtgcgcgag cacgtcgagg cgtacaagca gtccgcctgc ggcgagggcg
      361 acctgtacct gccggccgcg tgggcctacc tgcagatcgc cggcccggtc ctccagcgtg
      421 aggccgccga gcgtgacgcg acgaaggcca aggtgctcgc cgacctgtgg gccagcgacg
      481 cgccgaccga gcagcagctc gtcgatggca tcgcggcgct cgacgcgatc ttcccttgcg
      541 cctgacctgt ccctcgggac atcaacgcga gagaggtcgg gcgttcgccc gacctctctc
      601 gattcgtagg cggaacaggg atcgaacctg cgacccgcgc cgtgtaaagg cgccgctcta
      661 ccactgagct atccgcccgc tgtccccgga tggggccggc aagctaacac agttgctcgg
      721 cgacctgcgc gacgatcgtg aggtccgccg gggcgagcgc cgcgagcagc tcggaggtcg
      781 tccgcgtgcc acgaccgggc cgtagatccg gcgccagcgc ggccaggggg gccagcacga
      841 agtcccgccg ccccagctcc gggtgcggca gccgcagccc cggcgtctcg cactcgagct
      901 cgccgcccgc ggtcaaccac accaaaatat cgaggtccag cgtgcgcgcc tcccagcgct
      961 cgcgccggat ccgcccgtga cgcagctcga ggtcctgcaa ggcgctcaac agggcgtgcg
     1021 gctcggcgac cgtgcgcagc tcgatgaccg cgttgaggta cgcccaggtc gagggcccga
     1081 gcggccgcgt ctcgtagacc ggcgagctgc gcaccagcgc cgtgcccggc accgcgccgt
     1141 ctcgcagctc gcgcgcgacg ctctgcaagg tcgcgagccg gtcgccgagg ttgctgccga
     1201 gcgcgacgta caccgccgcc ggcgcggcga ggtcgtccgc gaggcccttg tgtgggagtc
     1261 gcgtcacgag gttcctgcgc gcccgggcga gcaccgcccc gtgctagccg tttgatccgc
     1321 ccgatgcaac ccattccccg ctttagcgcg tcaccgacat cgccgcgccg ctgctcggcg
     1381 ccagcatggt ctgatcgtat ggcaacacga tctcgatcgc cacgctgcgc ccgagcccga
     1441 cgtcgagcgc gccggaccag cggcgccgct gcggggccgc ggggccgctg tcggcgtcca
     1501 tgccgggcgg ctcgtccgcg ggcgctggcg gcacgggggg ccacagctcc ggccccggca
     1561 gcacgaagcg ggcgcggccc tcgaggtcgg tcgtcgcttg gctgcggtgc tcgccgagct
     1621 gcagcccgag cttgcgcacc gtcgccggca aggtcggctc gtcgcactcg accgccgggc
     1681 ccggtcgcag ctcgacgacg tcggaccggc ggcgatggtc gcgggcgcgg acgctgtcgt
     1741 agatgccggc cccgagcgcg accgtgccga gccccatgaa cacgccgccg agcttgtagc
     1801 cggtcttcgg gtcgcgcagc tcgccgccgg ggaccggcgt cggcacggcg aacaggtccg
     1861 gccgcgcgaa gctgaaggcc gccagcccga gcagcaccgt cgccagcccg tactcccagt
     1921 agatcgccgc gtcggcgcgg cgcgtgcggt gctcctcgcg ggccaccatc acccgctcga
     1981 ccacctgaca gcgccggtgc tcgagcagct cgaccgacag gctgtcgccg cgctgcaccc
     2041 accgcagctc gagctggcgc tcggtcaccg cgcgctcgct ccccagcagc cgcggcccgg
     2101 gcaccgccag cgcgtgcacc cgcgtcgtct ccgagatctg cgcgcaacct gcgagctgta
     2161 ccgcgacggc gaggcaggtc cagcgcgcca gcatcgagct gctacacggc gacccgtgtc
     2221 cccatcttgt cactgacgac gcgcgcgacc gtctgcatca gctcgtcgcc ggtgcgccgc
     2281 gcgacccacc gtccgtcgct ctcgtggtcg aagtgccacg cggtcgtgcc ggcggccatc
     2341 cacacctgca tcgccgcgct gtgggcgttg atgacgaagc gggcgccgtc ggcgaagctg
     2401 atcgtcagga cgtcgcctgc gtgctggatg tcgaggccgt cgaggtcggc ctcaccgagc
     2461 gccgtgtcga tgtgctcgag cgccttcgcc gagagcttgt gaaattcttt gacgtccatg
     2521 ctcgctcgca gccttacgcg atcaccccgg ggcggtcaat tcgcggcctc cgccggcggc
     2581 gtcggggtag cagtcggagc ctcggcggcc tttttgtcct cggccggcgg catcttgatc
     2641 ccgtgcaggc gctcggcctg ctccggggtc agctcgacga tgtcgatcga ccgcccgtcg
     2701 tgcagcgtca ccgagcgggt cgcccccttg agctcgacct cgcgggccgc ggccgcctcc
     2761 gcctccgcgt gcgcgtactc gttctcgctg cgccacttct ggtaggcccc gtagccgaag
     2821 taggcgaaca gcgggacgaa gatcagtagg cggatgagga gccccctgcg cgtcatgcct
     2881 cgactttgca ggaaccaccg ccgcgcagca aggcctgccc cgtccgcggc gcccccaccg
     2941 cgtgaaagcc cgctcagggc accgagacga acgcgacggt gcgcgaagtc gtcgcctcga
     3001 cgcggctgtc cgggtgcagg ccgccgctgc cgtcgacgag ctcgagcggg gcgctgtggg
     3061 tccgcgacag cgtcacgaac gcctcgcaca cgccgtccga gggcggcatc ttcggggtca
     3121 gctcgacctg tgaggcgccg acgacgacct cgccgtcgtc gggctcctcg agctcgatcg
     3181 gctcgccgag gcaggcatcg ccgtcgatct cgtcggcgac cttgacggcg atcttcgcct
     3241 cgccgccacg gggagggtcc caggtgatcg tcagcggcgc ggcccgggac agctcgctgt
     3301 cggccatcgg cgacgtgatc gcaaagcccg gcgcgtcgat cctggccgtg aactcggtgc
     3361 gcgcgtcgtc ggtgcgcagc tcgaagcggc gggcgagcgc ctgctgcccg ctgacggccg
     3421 agagctcggc ctcgtagatg accgcgccgc tcgggcgctt gacctccgtc atgtcgacgt
     3481 cgtcgacgcg caggccatgg tcctcacaca gcttgacggg gatcgatcca tcctcgaggt
     3541 cggggcggcg cagctcgacc tcgacgcgcg cgcgctcgcc gttgtcggtc acctgcgcgg
     3601 tcagcaggac gtcgtcgccg aggtcggcgc acgcctggga gccgccgcag ccgatcccct
     3661 gggccgacac gagcaccgca ccgagcacgc acaggacccg tcgcatgccg ccatcgtacc
     3721 agaaagcggg cgggcgccac cgcggctggg cgcgcctttt agccccacag caggggtttg
     3781 acgaccgcca tgatcacgat caacagcagc ggcaagatcg ggacctcgtt gatcaggcgg
     3841 cactgccgcg aggtcagccg acaggtgccc gcggccagct gcttgcgcac gacgccggca
     3901 taaaagtgat agctgaagag gatcgccagc ccgaacagct tgacctggaa ccacggctga
     3961 ccgaggtagc cggcg
//
LOCUS       DASWNJ010000815         2981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_367531, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000815 DASWNJ010000000
VERSION     DASWNJ010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367531"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1444
                     /locus_tag="VGB85_13780"
     CDS             <1..1444
                     /locus_tag="VGB85_13780"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY0135151.1"
                     /translation="ARADVLRRRAAELLTRVALHAPVAAKLPAWTLEDEVQALCRDTD
                     LAEERAVQLLRAALTYAPDLELAHDLLADRFHRLHVQAEQQRDPLSAARHEILLREHG
                     SSRYAEYLRGDGSLTLVTDPPGATAELHMFVREGRRLRPSLLRDLGATPVERLSLALG
                     SYLVVVRAPGRATVHYPVLIERLHHWDSVRPGDTREVPIYLPRVDELGPDECYVPAGW
                     FHSGGDPAARNSLPGRRLWLDGYVLRRFPVTIGEYLEFLNDLVATGRHGAAAQHAPPV
                     PAWEEGRSAEGHEPGVFRGADGRYVLDGPAHTLRWPVAMVNWEAANVYAAWFAARTGL
                     PWRLCAEMEHEKALRGVDARAFPWGEFLDPTFCCMRLSHQNSRALRAAVDEYPDDESV
                     YEVRGLAGNVHTWCADAMQMSGPTIVAGIPQLSDGSDIRGVGAGGTHRVVRGGSWRST
                     EDACRGAFREGPPAVYRDTVLGLRLARPLP"
     gene            complement(1476..2342)
                     /locus_tag="VGB85_13785"
     CDS             complement(1476..2342)
                     /locus_tag="VGB85_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135152.1"
                     /translation="MTWEYHLLKRQLGLVDDPLLHLFERFCERAYAAMDTMEWSWKFS
                     GSQVLANRVFWLGAGGHREATGRHLLWLTDALGLRAEGHPGGLAAWRELVGHGIERAL
                     VAYAGPNLATGRGPVVKFYLTLDPRRVAEQPELARPPGALVDDLSIPAATGVLRCHTV
                     ELGGAEASRMYYLFTGADHDDPATAALITRLAGPRGAELARLHPRSGVAFKADGTDML
                     GLALRATGAEGPHPAQILSPVLVPLLAAAGRSEALYERLDRVTWVTVPLDEDSLRLPR
                     VLREMNVYVRLR"
     gene            complement(2339..>2981)
                     /locus_tag="VGB85_13790"
     CDS             complement(2339..>2981)
                     /locus_tag="VGB85_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135153.1"
                     /translation="GLELVCGGSPMLRAALADQLGRVTPIPAIRIYLEYAQGPWWSAF
                     GWTNGYSVTDLPLHQVFYGMGVGADAQANARVLMASYADFNSAAFWSSLIDLNSFAAV
                     STYAQRQLQQLHQMQTTVPPNSWATRDWNHDPHGGAWHAWNPGVRVIDAIQQIRQPMP
                     GQPVFICGEAYSSMQGWIEGALMSTEAMLLDHFAMAWPSWLGADFDFGRERAL"
BASE COUNT          426 a         1076 c         1076 g          403 t
ORIGIN      
        1 cgcgcgcgcc gacgtgctgc gtcgccgcgc cgccgagctg ctgaccaggg tcgcgctgca
       61 cgccccggtc gcggccaagc tgccggcctg gacgctcgag gacgaggtgc aggcgctgtg
      121 tcgcgacacc gacctcgcgg aggagcgcgc ggtccagctg ctgcgcgccg ccctgaccta
      181 cgcgccggac ctcgagctgg cccacgacct gctggcggat cgctttcatc gcttgcatgt
      241 gcaggccgag cagcagcgcg acccgctgtc ggctgcccgc cacgagatcc tcctgcgcga
      301 gcacggcagc tcccgctacg ccgagtacct gcggggcgac ggctcgctga ccctcgtgac
      361 ggatccgccg ggcgcgaccg ccgagctgca catgttcgtg cgtgagggcc ggcgcctgcg
      421 cccgagcttg ctgcgcgacc tcggcgcgac gccggtcgag cgtctgtcgc tcgcgctggg
      481 cagctacctc gtcgtcgtgc gcgcgccggg ccgcgccacg gtccactatc cggtattgat
      541 agaacgcctg caccactggg acagcgtgcg ccccggcgac acccgggagg tgccgatcta
      601 tctcccgcgc gtcgacgagc tgggccccga cgaatgttac gtgccggcgg ggtggttcca
      661 cagcggcggc gatcctgccg cgcggaacag cctgcccggg cggcggctgt ggctcgacgg
      721 ttacgtgctg cgccgctttc cggtcacgat cggcgaatac ctcgagttcc tcaacgacct
      781 cgtcgccacc ggccgccacg gcgccgcggc ccagcacgcc ccgccggtgc ccgcgtggga
      841 ggagggccgc agcgccgagg gccacgagcc gggcgtgttt cgcggcgccg acggccgcta
      901 cgtgctcgac ggccccgccc acacgctgcg ctggccggtg gcgatggtca actgggaggc
      961 cgcgaacgtc tacgcggcgt ggttcgccgc gcgcaccggc ctgccgtggc ggctgtgcgc
     1021 cgagatggag cacgagaagg cgctgcgcgg cgtcgacgcg cgggcgttcc cgtggggcga
     1081 gttcctcgac ccgacgtttt gttgcatgcg cctcagccac cagaacagcc gcgcgctgcg
     1141 agccgcggtc gacgagtacc cggacgacga gagcgtatac gaggtgcggg gactcgccgg
     1201 aaatgtgcat acctggtgcg ccgacgcgat gcagatgtcc ggaccgacga tcgtcgccgg
     1261 catcccgcag ctctccgacg gctcggacat ccggggcgtc ggcgccggtg gtacgcacag
     1321 ggtcgtgcgc ggcggctcct ggcgcagcac cgaggacgcg tgtcgcgggg cgtttcgtga
     1381 ggggccgccc gcggtctatc gtgacaccgt gctcggcctg cgcctcgccc gaccgctgcc
     1441 ttgacgagct ccccggtcgc gcggccccgc gacgatcagc gcaggcgcac gtagacgttc
     1501 atctcgcgca atacccgggg caggcgcagc gaatcctcgt cgagcggcac ggtcacccag
     1561 gtgacgcggt ccaggcgctc gtacagcgcc tcgctgcggc cagcggcggc cagcagaggg
     1621 acgagcaccg gggacaggat ctgcgccggg tggggcccct cggcgccggt ggctcgcaag
     1681 gcgagaccga gcatatcggt gccgtccgcc ttgaacgcga cgcccgagcg gggatgcagc
     1741 ctcgccagct cggcgccccg cgggcccgcc agtcgggtga tcagggccgc ggtcgcggga
     1801 tcgtcgtggt cggccccggt gaacaggtaa tacatgcgcg acgcctcggc gccgccgagc
     1861 tcgacggtat ggcagcgcag cacgccggtc gccgcaggga tcgagagatc gtcgaccagc
     1921 gcgccgggcg ggcgggccag ttccggctgc tcggcgaccc ggcgcgggtc gagggtcaga
     1981 tagaacttca cgaccgggcc gcggcccgtc gcgaggttgg ggcccgcgta ggccaccagg
     2041 gcgcgctcga tgccatgacc gaccaattcc cgccacgccg cgaggccgcc ggggtggccc
     2101 tcggcgcgca gacccagcgc gtccgtgagc cacaacaggt gccggcccgt cgcctcgcgg
     2161 tggccgccgg cgccgagcca gaacacccga ttcgcgagca cctgcgagcc cgagaatttc
     2221 cacgaccatt ccatcgtgtc catggccgca tatgcgcgct cgcaaaagcg ctcgaacagg
     2281 tgcagcagcg ggtcgtccac caggccgagc tgccgcttca ggaggtggta ttcccacgtc
     2341 acagggcgcg ctcgcggccg aagtcgaagt cggcgccgag ccagctcggc caggccatgg
     2401 cgaagtgatc gagcagcatg gcctcggtgg acatcagcgc gccctcgatc cagccctgca
     2461 tcgacgaata ggcctcgccg cagatgaaca ccggctgtcc gggcatcggc tggcggatct
     2521 gctggatcgc atcgatgacc cgtacacccg ggttccacgc gtgccaggcc ccgccgtggg
     2581 ggtcgtgatt ccagtcgcgg gtcgcccagg agttcggcgg caccgtcgtc tgcatctggt
     2641 gcagctgctg gagctgccgc tgcgcgtagg tgctcacggc cgcgaagctg ttgaggtcga
     2701 tcagcgagga ccagaacgcc gcggagttga aatccgcgta ggaggccatc aacacccgcg
     2761 cgttggcctg cgcgtcggcg ccgacgccca ttccgtaaaa gacctggtgg agcgggaggt
     2821 cggtgaccga gtagccgttc gtccagccga acgcgctcca ccacggaccc tgcgcatact
     2881 cgagataaat gcggatcgcg gggatcgggg tcacgcggcc gagctgatcc gcgagcgccg
     2941 cgcgcagcat cggcgagccg ccgcacacca gctccagccc g
//
LOCUS       DASWNJ010000816         5687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_685401, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000816 DASWNJ010000000
VERSION     DASWNJ010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685401"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /locus_tag="VGB85_13795"
     CDS             complement(<1..1419)
                     /locus_tag="VGB85_13795"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0135154.1"
                     /translation="MDAVTPPPPGPPLAPLRLRHSLAARIIALVVLVLVATGGASYLI
                     VAQLRALQASFDLLTIVYVKFNKDLTVAYRQSTRVASRIEFMTERRGEADRPLSPAEE
                     ANFIEALEERLLQVQRARSVLDDAFANLDRVGGQESLGPLRNLHNALAKLEEQVVQTT
                     TAAPADVIGDVRSQNAINESFRRLDDQVSRDVVELGNKVRDTERDTERMIVLLTLVIA
                     LLGMIAALGVVWTVRPLRRLALGVRELGRGDWAQRVLVDSPTRGDEVSQLAREFNQMA
                     AALQERERRLIFGERLAAVGQLAAQVTHEIRNPLSSVALNVELLADEIAATSPEAHQL
                     LGKITQEIDRLTSITEDYLGFVRRKNPERVRLDLAAELQSLLDFMAEELAAAGIRVVR
                     QLRPAWVLGDAGQLRQAFMNLLRNAREALAEGPPPPQNPIGDLLDGEEDPEPAAAGDL
                     PPERARPADDPGARSPSVTVRVE"
     gene            1621..2625
                     /locus_tag="VGB85_13800"
     CDS             1621..2625
                     /locus_tag="VGB85_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135155.1"
                     /translation="MTALSLLAGWVNPAQAAGPPSGKASVLTLEGDDTARAQALSKAL
                     EAEFSARGVGGGREMSLAELKLTMGCDEPPALACLSSGGKTLGLDSMIYGSLSKTKTG
                     AFALKLSVMDVASSTVSQSVNTELSADAVSDANVRSTAKDIVTQVLGPEVKAAPEPEP
                     EPVVEAPEDNKKKLVWGRHDAAKWKKAGLAASAALTVVSLGVAIGTYMVIRPGGKLKK
                     DLVDAANRSQMDESSTNDINPYTKNDLCDEARSQVSGGVKNGEMTKICNKADGLATAA
                     TAGFIATGIFAASTIVFTTLLFVHRDKPGVAKLQKHGLTLGAMPLRSGGVMVGGVVRF
                     "
     gene            complement(2635..4047)
                     /locus_tag="VGB85_13805"
     CDS             complement(2635..4047)
                     /locus_tag="VGB85_13805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY0135156.1"
                     /translation="MTAACALSLAVGCGNMLGNNKAREAKTEVPGSFGALADDPGPSV
                     AVQQRWDTFFADPDLRLLIKAALENNQQLNIQLQELILNQNNIAALRGEYIPRLDAMV
                     GAGVAKDGEYTPEGVSHTAHGIREPIPDFRFGFVASWETDIWSRLRNARKAAVLHYEA
                     TIQERNFLITEIVAEIANSYYDLVALDVQIEILDRNIALQKNALEIVKLKKAAAHDTE
                     LGVQRFRAEVLKNQGRRFSLEQQRILVENRINFLVGRFPQHVARNSQNFMVANTDMVS
                     TGLPSDLLENRPDVKAAELELEASKLDTRSAKARFYPSLRLDAGVGFESFNLKHLINT
                     PQSLAYNLAGSLAAPLLNRAAIKADYRSANARQIQAVFEYERSILQAFTDVYNQMTAI
                     SNLRQRYQQLSEQVDAMHSAIEVSRALYQSAQADYSEVLLTTRDSLEAEMELVEAKKD
                     QLQALVAIYQALGGGWRQDP"
     gene            complement(4070..>5687)
                     /locus_tag="VGB85_13810"
     CDS             complement(4070..>5687)
                     /locus_tag="VGB85_13810"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0135157.1"
                     /translation="AGFVPNEDQGIIYAIIQTPPGTTLERTNAVARTLQDIALEIDDI
                     ASVSSLAGYEIITEGRASNAGTCLINLKDWSERDHSVNEVIEELEEKAKDIGAVVEFF
                     EPPAVPGYGAAGGVSFRMIDKTGSTDYQAFDRINQELMAGLRERPELSGLFTFFAANY
                     PQYEIIIDNEKAMQKGVSIGKAMENLDLMIGSSFETGFIRFGFFYRAYVQALPQFRAL
                     PSDVLKMYVKNEAGEMVPYSSFMTLKKRQGPNELTRYNMYNSAAIRGNNATGYTTGDA
                     VKAIKEVAAEVLPRGYDIAWEGLSYDEARRGNEALYIFGIVLVFVYLVLAAQYESFIL
                     PLAVITSLPAGAFGSFMFLQMMGLANDIYAQVGLVMLVGLLGKNAVLIVEFAVQTRRE
                     GASVFEAAVAGARARFRAILMTSFAFVAGLYPLVVAKGAGAVGNNTIGSSALGGMVIG
                     TVFGVILIPGLYFIFGHLAHYERFLRIGSPTRPQPLSEAMAPAPETWSPPVAVTHATV
                     ADDAPVAVRPVALSETPAHADARREEESGE"
BASE COUNT          862 a         1850 c         2061 g          914 t
ORIGIN      
        1 ctcgacgcgc acggtgacgc tcgggctgcg ggcgccaggg tcgtccgcgg gccgagcgcg
       61 ttccggtggc agatcgccgg ccgcggccgg ctccgggtcc tcttccccat cgagcaggtc
      121 gccgatcggg ttctgcgggg gcggtgggcc ctcggcgagc gcctcccggg cgttgcgcag
      181 caggttcatg aaggcttgcc gcagctggcc ggcgtcgccg aggacccagg ccgggcgcag
      241 ctggcggacg acgcggatgc cggccgcggc gagctcctcg gccatgaagt cgagcaggct
      301 ctggagctcg gcggcgaggt cgaggcggac gcgctcgggg ttcttgcggc ggacgaagcc
      361 gaggtagtcc tcggtgatcg aggtgaggcg gtcgatctcc tgggtgatct tgccgaggag
      421 ctggtgggcc tcgggggagg tggcggcgat ctcgtcggcg agcagctcga cgttaagggc
      481 gacgctcgac agcgggttgc ggatctcgtg ggtgacctgg gcggcgagct ggccgacggc
      541 ggcgaggcgc tcgccgaaga tcagccggcg ctcgcgctcc tgcagggcgg cggccatctg
      601 gttgaactcg cgggcgagct ggctgacctc gtcgccgcgg gtcgggctgt cgacgagcac
      661 gcgctgggcc cagtcgccgc ggccgagctc gcggacgccg agcgcgaggc gccgcagcgg
      721 ccgcacggtc cacacgacgc cgagcgcggc gatcatgccg agcagggcga tcaccagggt
      781 cagcaggacg atcatgcgct cggtgtcgcg ttcggtatcg cgcaccttgt tgccgagctc
      841 gacgacgtcg cggctgacct ggtcgtcgag gcggcgaaac gactcgttga tggcgttttg
      901 gctgcggacg tcgccgatca cgtcggccgg ggcggcggtg gtggtctgga cgacctgctc
      961 ctcgagcttg gcgagcgcgt tgtggaggtt gcgcagcggt cccagcgact cctggccgcc
     1021 gacgcggtcg aggttggcga aggcgtcgtc gagcacggag cgggcgcgct ggacctgcag
     1081 caggcgctcc tcgagggcct cgatgaagtt ggcctcctcg gcggggctca gcgggcggtc
     1141 ggcctcgccg cggcgctccg tcatgaattc gatgcgcgag gcgacgcggg tcgactggcg
     1201 ataggcgacg gtgagatcct tgttgaactt gacgtagacg atcgtcagca ggtcaaagct
     1261 ggcctgaagc gcgcgcagct gggcgacgat cagataggag gcgcccccgg tcgcgaccaa
     1321 aaccaggacc acgagcgcga tgatgcgggc cgcgaggctg tggcgcaggc gcagcggggc
     1381 cagcggcggg cccgggggcg gcggtgtgac ggcgtccacg cgccgcgatg gtacaggggc
     1441 ctgtccgcga acgcaccgcc gggggccagc ggcgacgcgc cccccggccc gttttcgggg
     1501 gagcgcgccg cgttttgtcc tgcccccttc cgcggggccc cggggcgtgc gaacgttgcg
     1561 tcgaccatgt cgaagctgtc gatgccgagc cgccgtccgt cctcgtccct gctcgctggc
     1621 gtgaccgcgc tgtccctgtt ggcgggctgg gtgaacccgg cccaggccgc gggtccgccg
     1681 tcggggaagg catcggtgtt gacgctcgag ggcgacgaca cggcgcgcgc acaggcgctg
     1741 agcaaggcgc tcgaggcgga gttctcggcg cgcggcgtcg gtggtggccg cgagatgtcg
     1801 ctggccgagc tcaagctgac gatgggctgc gacgagccgc ccgcgctggc gtgcctcagc
     1861 agcggcggca agaccctcgg cctcgacagc atgatctacg ggtcgctgtc gaagaccaag
     1921 acgggggcct tcgcgctcaa gctcagcgtg atggacgtcg cctcgtcgac ggtgtcgcag
     1981 tcggtcaaca ccgagctcag cgccgacgcc gtcagcgacg ccaacgtgcg ctcgaccgcc
     2041 aaggacatcg tcacgcaggt gctcggcccg gaggtcaagg cggcgcccga gcccgagccc
     2101 gagccggtgg tcgaggcgcc cgaggacaac aagaagaagc tggtgtgggg ccgccacgac
     2161 gccgccaagt ggaaaaaggc cggcctggcc gcctcggcag ccctgacggt cgtctcgctc
     2221 ggcgtggcga tcgggacgta catggtcatt cgcccgggcg gcaagctcaa gaaggacctc
     2281 gtcgacgccg ccaatcgcag ccagatggac gagagctcga ccaacgacat caacccctac
     2341 accaagaacg acctgtgcga cgaggcccgc tcgcaggtct ccggcggggt caagaacggg
     2401 gagatgacga agatctgcaa caaggccgac ggcctggcga ccgccgccac cgcgggcttc
     2461 atcgcgaccg gcatcttcgc cgcctcgacg atcgtgttca cgacgctgct gttcgtccac
     2521 cgcgacaagc ccggcgtcgc caagctgcaa aagcacgggc tcacgctcgg cgcgatgccg
     2581 ctgcgcagcg gcggcgtgat ggtcggcggg gtcgtccgct tttagtccgg gccctcacgg
     2641 gtcctgtcgc cagccgccgc cgagcgcctg gtagatcgca acgagcgcct gcagctggtc
     2701 cttcttggcc tcgacgagct ccatctcggc ctcgagcgag tcgcgtgtcg tcaggaggac
     2761 ctcggagtag tcggcctgcg ccgattgata gagggcccgc gagacctcga tcgccgagtg
     2821 catcgcgtcg acctgctcgg acaattgctg gtagcgctgc cgcaggttcg agatcgcggt
     2881 catttgatta tagacatcgg tgaacgcctg caggatcgat cgttcatact cgaagaccgc
     2941 ctggatctgc ctggcgttgg ccgacctgta gtccgccttg atcgcggcgc ggttgagcag
     3001 cggcgccgcc agactgccgg ccaggttgta ggcgagcgac tgcggcgtat tgatcagatg
     3061 cttcaaattg aaggactcga agccgacgcc ggcgtcgagg cgcagcgagg ggtagaaccg
     3121 ggccttcgcg ctccgggtgt cgagcttcga cgcctcgagc tcgagctcgg ccgccttgac
     3181 gtcggggcgg ttctccagca agtccgaggg caggccggtc gacaccatat cggtgttcgc
     3241 taccatgaag ttctgcgagt tgcgcgcgac gtgctgcggg aagcgcccga ccaggaagtt
     3301 gatgcggttc tcgacgagga tccgctgctg ctcgagggag aagcgccgcc cctggttctt
     3361 gagcacctcg gcgcggaacc tctgcacacc gagctcggtg tcgtgcgcgg cggccttctt
     3421 gagcttgacg atctcgaggg cgttcttctg cagggcgatg ttgcggtcga ggatctcgat
     3481 ctggacatcg agcgcgacga ggtcgtagta ggagttggcg atctccgcga cgatctcggt
     3541 gatcaggaag tttcgctcct ggatcgtcgc ctcgtagtga agcaccgcgg ccttgcgggc
     3601 gttgcggagc ctgctccaaa tgtcggtctc ccacgacgcc acgaagccga agcgaaagtc
     3661 ggggatcggc tcgcggatgc cgtgggcggt gtgactgacg ccctcggggg tgtactcgcc
     3721 gtccttggcg acgcccgcgc cgaccatcgc gtccagccgc ggtatgtact cgccgcgcag
     3781 cgccgcgatg ttgttctggt tgaggatgag ctcctgcaat tggatgttga gctgttggtt
     3841 attctccagc gccgccttga tcaacagccg gagatcgggg tcggcgaaga aggtgtccca
     3901 gcgctgctgc accgcgacgc tcgggccggg gtcgtcggcg agggcgccga acgacccggg
     3961 gacctccgtc ttcgcctcgc gcgccttgtt gttgccgagc atatttccgc agccgaccgc
     4021 gagcgacagg gcgcaggcgg ccgtcacggc gccgatcgta cgttgccacc tactcaccgg
     4081 actcctcctc gcgccgcgcg tcggcgtgcg ccggggtttc ggacagggcc acggggcgga
     4141 ccgccaccgg cgcatcgtcg gcgaccgtcg cgtgcgtcac cgcgaccggc gggctccacg
     4201 tctcgggcgc gggggccatc gcctcggaga gcggctgcgg tcgggtcggg ctgccgatcc
     4261 gcaggaagcg ctcgtagtgc gcgaggtgac cgaagatgaa gtaaagcccc gggatgagga
     4321 tcacgccgaa cacggtgccg atcaccatgc cgccgagcgc ggacgagccg atcgtgttgt
     4381 tgccgaccgc ccctgccccc ttcgccacga ccagcggata caggccggcg acgaacgcga
     4441 acgaggtcat caggatcgca cggaagcggg ccctcgcgcc cgcgacggcc gcctcgaaca
     4501 cgctggcgcc ctcacgccgg gtctggaccg cgaactcgac gatcagcacc gcgttcttgc
     4561 cgagcaggcc gaccaacatg acgaggccga cctgggcgta gatgtcgttc gccaggccca
     4621 tcatctgcag gaacatgaac gacccgaacg cgcccgccgg caacgacgtg atcacggcca
     4681 gcgggaggat gaagctctcg tactgcgccg cgagcacgag atacacgaac accagcacga
     4741 tcccgaagat gtacagcgcc tcgttgccgc gccgcgcctc gtcgtaggac agaccctccc
     4801 atgcgatgtc gtagccgcgc ggcaggacct ccgccgccac ctctttgatc gccttgacgg
     4861 cgtcaccggt cgtgtagccg gtcgcgttgt tgccgcggat cgccgccgag ttgtacatgt
     4921 tgtagcgggt gagctcgttg gggccctgcc gcttcttgag cgtcatgaac gacgagtacg
     4981 ggaccatctc gccggcctcg ttcttgacgt acatcttgag cacgtccgag ggcagcgcgc
     5041 gaaactgcgg caacgcctgc acgtaggcgc gatagaagaa gccgaagcgg ataaagccgg
     5101 tctcgaacga gctgccgatc atcaggtcga ggttctccat cgccttgccg atcgagacgc
     5161 ccttttgcat cgccttctcg ttgtcgatga tgatctcgta ttgcgggtag ttggcggcga
     5221 agaaggtgaa gaggccggac agctcgggcc gctcgcgcag gcccgccatg agctcctggt
     5281 tgatgcggtc gaaggcctgg tagtcggtgc tgccggtctt gtcgatcatg cggaaggaga
     5341 cgccgccggc cgcgccgtag cccgggaccg cgggcggctc gaagaactcg accaccgcgc
     5401 cgatgtcctt ggccttctcc tcgagctcct cgatgacctc gttgaccgaa tggtcgcgct
     5461 cggaccagtc cttgaggttg atgaggcagg tccccgcgtt ggaggcgcgc ccctcggtga
     5521 tgatctcgta gccggcgagg gacgagaccg acgcgatgtc gtcgatctct agggcgatgt
     5581 cctgcagggt ccgtgcgacc gcgttggtcc gctcgagcgt ggtgcccggc ggggtctgga
     5641 tgatcgcgta gatgatgccc tggtcctcgt tcggcacgaa gccggcc
//
LOCUS       DASWNJ010000817         2275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_120633, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000817 DASWNJ010000000
VERSION     DASWNJ010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120633"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VGB85_13815"
     CDS             complement(<1..260)
                     /locus_tag="VGB85_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135158.1"
                     /translation="MSAPEFARHLRAAGPDEHRCRRLVLAYVRRYQETYWFEDLEPAI
                     AALERVGGEPGRLGDEGEAVRADVELALHEYCLSLNEIAAAL"
     gene            complement(257..1237)
                     /locus_tag="VGB85_13820"
     CDS             complement(257..1237)
                     /locus_tag="VGB85_13820"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY0135159.1"
                     /translation="MPCSLKILATARALPPRVLTNAELEAMVETSDSWIRERTGIRER
                     RIAAPDVATSDLAAEAVLAACERAGIAASALDAIIVGTSTPDTLFPSTACWLQHKLGL
                     RGPAAFDVSAGCSGFLYGLEIASGLIHGGARHIAVVGAEVMSRVVNWQDRSTCVLFGD
                     GAGAAILGPGDGSSGILGSAWGADGNLAKVLYQPAGGTRAPASTETVAALAHTVHMEG
                     NTVFKHAVTAMSEAATQAMARAGITPDQVDLLIPHQANTRIMEAARARTGVPAERLFS
                     IVERYGNMSAATVPVALDEAREQGRIKDGDIVVMTAFGTGFTWAASVLRW"
     gene            1450..>2275
                     /locus_tag="VGB85_13825"
     CDS             1450..>2275
                     /locus_tag="VGB85_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135160.1"
                     /translation="MLRTRSVLPRSSWLVPAVGSVLALVLALGGCASSLERARVARDN
                     RDYPKAEELYRGAMAKDPEDKARAQMELGSLKVALAQNKLKKDPVAAEKLFREALELN
                     PRDDKAIDGLGRAQAEQGKQDAAISTLSAPGCTLCRRYLSVLLLDRAGKREQAADLEG
                     ARGDYIKAQELVPDVTTALAIARLYEAARDGEGLLKAVEAAVPLIRQDDGVAQAQFKA
                     LREKAVMAAAQTADVTTIDRWLNLFPPGAGGDEWYVLQLRVAQQLYRGGHTDLAVAR"
BASE COUNT          332 a          780 c          874 g          289 t
ORIGIN      
        1 cacagcgcgg cggcgatctc gttgaggctg aggcagtact cgtgcaacgc cagctcgacg
       61 tccgcgcgca ccgcctcgcc ttcgtcgccg aggcgcccgg gctcgccgcc gacccgctcg
      121 agggccgcga tcgcgggctc gagatcctcg aaccagtagg tctcctggta gcgacgcacg
      181 tacgcgagca cgagccgccg gcagcgatgc tcgtcgggcc ccgcggcgcg caggtgccgg
      241 gcgaattccg gcgcgctcac cagcgcagga ccgacgcggc ccaggtgaag ccggtgccga
      301 aggcggtcat cacgacgatg tcgccgtcct tgatccggcc ctgctcgcgg gcctcgtcga
      361 gggcgaccgg gacggtcgcg gcggacatgt tgccgtagcg ctcgacgatc gagaacaggc
      421 gctcggccgg gacgccggtg cgcgcgcggg cggcctccat gatccgcgtg ttggcctggt
      481 gcgggatcag gagatcgacc tggtcgggcg tgatgccggc gcgggccatc gcctgggtcg
      541 ccgcctcgct catcgcggtg acggcgtgct tgaacaccgt gttgccctcc atgtgcacgg
      601 tgtgggccag cgccgcgacg gtctcggtcg aggcgggcgc ccgcgtgccg cccgcgggct
      661 gatagaggac cttggcgagg ttgccgtcgg cgccccaggc cgagccgagg atccccgagc
      721 tgccgtcgcc ggggccgagg atcgccgcgc cggcgccgtc gccgaacagc acgcaggtgc
      781 tgcggtcctg ccagttgacg acccggctca tcacctcggc gccgacgacc gcgatgtgcc
      841 gggcaccacc gtggatcaga cccgaggcga tctcgagacc ataaaggaag cccgagcagc
      901 cggcgctgac atcgaaggcg gccgggccgc gcaggccgag cttgtgctgg agccagcacg
      961 cggtcgaggg gaacagcgta tcgggggtcg aggtgccgac gatgatcgcg tcgagggccg
     1021 aggccgcgat gccagcccgc tcgcaggcgg cgagcacggc ctcggcggcg aggtccgagg
     1081 tggcgacgtc cggcgcggcg attcggcgct cacggatccc cgtgcgctcg cggatccagg
     1141 agtccgaggt ctcgaccatc gcctcgagct cggcgttggt gagcacgcgc ggcgggaggg
     1201 cgcgggccgt ggcgaggatc ttgagcgagc agggcattgt taatgaagtt ccgcgacgac
     1261 cggtgctatc ccgtcgcgcg cgggccggca agcgggggcg gccgggacga ctaccataaa
     1321 aacgcgctgg ctcgatcctg gagccataaa aacggcctaa ccgtgacctg tgggccgcag
     1381 cgatccgccg ccggaagcgc cgcagctctg cgaaagcgcg cccgggcctg gtagagtgcg
     1441 gacggccgca tgctccgcac tcgatccgtc ctgccccgtt cgtcctggct cgtccccgcc
     1501 gtcggctccg tgctcgcgct ggtgctcgcg ctcggtggct gcgcctcgtc gctcgagcgg
     1561 gcccgcgtcg cgcgggacaa ccgggattac cccaaggcgg aggagctcta ccgcggcgcg
     1621 atggccaagg acccggagga caaggcccgg gcgcagatgg agctcgggtc gctcaaggtg
     1681 gcgctggcgc agaacaagct caagaaggac ccggtcgccg cggagaagct gttccgcgag
     1741 gcgctggagc tcaacccgcg cgacgacaag gcgatcgacg gcctcggccg ggcgcaggcg
     1801 gagcagggca agcaggacgc cgcgatcagc accctgagcg cgcccggctg cacgctgtgc
     1861 cggcgctacc tcagcgtgct gctgctcgac cgcgcgggca agcgcgagca ggcggccgac
     1921 ctcgaggggg cgcggggcga ctatatcaag gcgcaggagc tggtgcccga cgtcacgacg
     1981 gcgctggcga tcgcaaggct gtacgaggcg gcgcgtgacg gggagggcct gctcaaggcg
     2041 gtcgaggcgg cggtcccgct gatccgccag gacgacggcg tggcccaggc gcagttcaag
     2101 gcgctgcgcg agaaggcggt gatggccgcg gcgcagacgg ccgacgtcac gacgatcgat
     2161 cgctggctca acctgttccc gccgggcgcc ggcggcgacg agtggtacgt gctgcagctg
     2221 cgggtcgcgc agcagctgta tcgcggcggt cacacggacc tcgcggtcgc ccggg
//
LOCUS       DASWNJ010000818         3011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_651078, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000818 DASWNJ010000000
VERSION     DASWNJ010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651078"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VGB85_13830"
     CDS             complement(<1..485)
                     /locus_tag="VGB85_13830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0135161.1"
                     /translation="MIEKIDAIIAEHGVTYVPSSSGEDSGHADTSTSASTGTSTGGVV
                     GSESDGHGGTLESSTTAQAETQAASDGSSSGGGPVCGDGVVEGDETCDDGNATPDDGC
                     QECARDSIVFITSESYQGYALEGLYGADQRCRSLAAKAYLGRPESFRAWLSTPSMAAA
                     DR"
     gene            complement(574..1800)
                     /locus_tag="VGB85_13835"
     CDS             complement(574..1800)
                     /locus_tag="VGB85_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135162.1"
                     /translation="MSSSITLPSPIRSWSTSPLPVGLLSIWLAVACNHDVSAPTGDFE
                     VGASTSSSTAPDSPLTGAVPTTSGAESTTTSAGAGETTIGDTGTTGTTGTTGTTGEPG
                     TTGEPGTTGEPGDSTGDAPSPACGDGQLDPGEQCDPGLLQLSQTGACTLSCQTAKCGD
                     NLIWEGHEACDNGPGNNDELYGGCTSDCQLGPACGDSQVQGPEECDLGIDNGTGEFPP
                     TGVPCDSGCRFQARLVFLSSTVYQGGEIGGVEGAHIECRNRAKLAKYDNATKFMAWIS
                     DAQHSPHQDFAHGPETAGLAYVRPDGVRVADDWDALVLKGLIEGIAVTETGEVLLDKR
                     VWTGTAPSGKVLDPAAHCESWTSSSGNQKSRVGSSGVDKVKYPQAWAQWVADRQWTNL
                     FSWTCDEALHLYCVEQ"
     gene            complement(2019..2156)
                     /locus_tag="VGB85_13840"
     CDS             complement(2019..2156)
                     /locus_tag="VGB85_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135163.1"
                     /translation="MASEHMLVNAAKIPDAAARRRFLEAIPEHARIPALAGAWREAAD
                     D"
     gene            2260..2412
                     /locus_tag="VGB85_13845"
     CDS             2260..2412
                     /locus_tag="VGB85_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135164.1"
                     /translation="MRLTRQTLIELSLHPNNNVSGIFGPRCRKHVGLEDSVVFARTRR
                     PTPRWG"
     gene            2577..>3011
                     /locus_tag="VGB85_13850"
     CDS             2577..>3011
                     /locus_tag="VGB85_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135165.1"
                     /translation="MSTEDQELVRRVVQGDAAATRTLVSRLLPVVAREVGFSLLRRAA
                     ATRRDPRQEQHDLIQDVLILLLASSGRELARWDPGRGRSLDSFVQLITRRRVARTFAG
                     GRNNPWSDDPVAPEDLEHHDDDTRELAALEARNELGGVLERLH"
BASE COUNT          418 a         1146 c          953 g          494 t
ORIGIN      
        1 cgatcggccg ccgccatcga tggggtgctc aaccacgccc gaaagctctc gggccgcccc
       61 agatatgcct tcgccgccag actgcggcac cgctgatccg caccgtacag cccctccagc
      121 gcgtatccct ggtagctctc gctcgtgatg aacacgatcg agtcccgcgc gcactcctga
      181 cacccatcgt ccggcgtcgc gttcccgtca tcacaggtct cgtcaccctc gaccacccca
      241 tcgccgcaga ccggcccccc tccgctactg ctgccgtccg atgctgcttg cgtctccgcc
      301 tgagctgtcg tggacgactc caaagtaccg ccgtggccgt cgctctcgga gcccacgacc
      361 ccgcccgtgc tcgtacccgt gctggcgctc gtgctcgtgt cagcgtgacc gctgtcctca
      421 ccggagctcg atggcacgta ggtgacgccg tgctcggcga tgatcgcgtc gatcttctcg
      481 atcacacacc ctccgccgcc gagcgccccg caaagcatca gcgcacccgc ggcccgacga
      541 ctcgctcgat tcatcatggt ccggtgcatc ctctcactgc tccacacaat atagatgtaa
      601 cgcctcgtcg cacgtccaac tgaacaggtt cgtccactgc cggtcggcta cccactgcgc
      661 ccaggcctgc gggtatttca ccttgtcgac cccgctgctt ccgacgcggc tcttctgatt
      721 tccggacgag ctggtccagc tctcgcagtg cgcggccggg tcgagcacct tcccgctcgg
      781 ggcggtgccg gtccagaccc gcttgtcgag cagcacctca ccggtctcgg tcaccgcgat
      841 gccctcgatc agccctttca ggaccagcgc gtcccagtcg tcggccaccc ggacgccgtc
      901 cggccgcaca tacgccaggc cggcggtctc ggggccgtgg gcgaagtcct ggtgcgggct
      961 gtgctgcgcg tcgctgatcc aggccatgaa cttcgtcgcg ttgtcgtatt tggccagctt
     1021 ggcgcggttc cggcactcga tgtgcgcccc ctcgacaccg ccgatctcgc cgccctggta
     1081 gacggtgctg ctcaaaaata ccagcctggc ctggaagcgg cagccgctgt cacacggcac
     1141 ccccgtcggc gggaactcgc cggtcccgtt atcgatgccg aggtcgcact cctcgggccc
     1201 ctggacctgg ctgtctccgc acgcaggtcc aagctggcaa tcgctggtac acccgccata
     1261 gagctcgtca ttgttgcccg gcccattatc gcaggcctca tgcccctccc agatcagatt
     1321 gtcgccacac ttcgcggtct ggcacgagag cgtgcaggcg cctgtctgac tgagctgtaa
     1381 cagccccggg tcgcactgct cgccggggtc gagctggccg tcgccgcagg ccggggacgg
     1441 ggcatcgccc gtcgagtcgc cgggctcgcc cgtcgtgccc ggctcgcccg tcgtgcccgg
     1501 ctcgcccgtc gtgcccgtcg tgcccgtcgt gcccgtcgtg cctgtatcgc cgatcgtcgt
     1561 ctcgcccgcg cccgcggatg ttgtcgtgct ctccgcgccg ctcgtcgtcg gcacagcgcc
     1621 cgtcagaggc gaatccgggg ccgtgctgga cgacgtactc gcgccgacct cgaagtcccc
     1681 ggtgggcgcg ctgacgtcat gattgcaggc cacggcgagc cagatcgaga gcagcccgac
     1741 aggcagtggg gacgtcgacc acgaacggat cgggctcggc agagtgatgg agctggacat
     1801 gaatggacct ccctggtcgc acgcgcgatc tcgcgccgct gaccatagcg gtccgggccg
     1861 ccggtgtcca gcgtcatcgt ggccgtgcac acatcgagcg ccgcaacgcg tggggcgcag
     1921 gcaaaacatc gttaccgggg agccggcggt cgccggtcac ttcacggctt gcacgcgagc
     1981 gtccatcgcg gtcatgcaca ggcccgtggc tggacccatc aatcgtcggc cgcctcgcgc
     2041 cacgccccgg cgagggcggg gatccgcgcg tgctccggga tggcctcgag gaagcggcgg
     2101 cgcgccgcgg cgtcggggat cttggcggcg ttgacgagca tatgctcgct cgccaccgcg
     2161 accgcggccg tcgccgcggc acgatcacca gccggccaac atggaccgtt acatgtactt
     2221 tggcacgctg ctgaccctga gcgactcctt cggggagcca tgcgcctcac ccggcagacg
     2281 ctgatcgagc tgtctctcca cccgaacaac aacgtgtcgg gcatcttcgg tccgcgctgc
     2341 cggaagcacg tcggcctcga ggactccgtg gtgttcgcgc gcacaagacg cccaacgccg
     2401 cgctggggct gatcagcgtg catgacgggc tgttccagtg gctcgtggcc cccgccggca
     2461 acaatcacac gatcgacaac gggagcctcg ccgccgagct cagcgtcggc ccaggctgct
     2521 gacgagccga cgcggcgtct gctcgccctg cgcgaggtat ggtagcgtcc ggcgccttgt
     2581 cgaccgagga ccaggagctc gtacgccgcg tcgtccaggg ggacgccgcg gcgacgcgta
     2641 cgctggtctc ccgcctgctc ccggtcgtcg cccgcgaggt cggcttctcg ctgctgcggc
     2701 gcgcggcggc gactcgccgc gacccccgcc aggagcagca cgacctcatc caggacgtcc
     2761 tgatcttgct gctcgccagc agcgggcgcg agctggcccg gtgggacccc gggcgcggtc
     2821 gctcgctcga cagcttcgtc cagctgatca cccgccgccg ggtcgcccgc acgtttgccg
     2881 gcggtcgcaa caacccgtgg agcgacgatc ccgtcgcccc cgaggacctc gagcaccacg
     2941 acgacgacac ccgcgagctc gccgcgctcg aggctcgcaa cgagctcggc ggcgtgctcg
     3001 agcgcctgca c
//
LOCUS       DASWNJ010000819         4642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_651083, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000819 DASWNJ010000000
VERSION     DASWNJ010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651083"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VGB85_13855"
     CDS             complement(<1..518)
                     /locus_tag="VGB85_13855"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY0135166.1"
                     /translation="MQLTIAITIFVAGFLAGVVNAVAGGGTFITFGALTVIGVSPILA
                     NTTSSLTQFPGYIASALAYRAEIQQQWRAALALCVASIVGATLGSWLLLALDNASFRA
                     LVPWLLLAATLLFAAGPWLKPRPREGQAAPGPAVGLVVQGATAVYGGFFGAGVGVMML
                     ATLGLTRPGDYH"
     gene            complement(536..715)
                     /locus_tag="VGB85_13860"
     CDS             complement(536..715)
                     /locus_tag="VGB85_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135167.1"
                     /translation="MANPKTPDRADEDTKGGLSRAGQDGKDQDSNYAEDDSAGPGTRG
                     SELPEPGQGEKSGQR"
     gene            1004..1792
                     /locus_tag="VGB85_13865"
     CDS             1004..1792
                     /locus_tag="VGB85_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135168.1"
                     /translation="MQDARDEDLPDPRPNERDVMNPSPAPAPGPDLAAEGRLTARPGA
                     PGAPGDAASAGTYRLGLTTTRDGLIHVPAAIDAGKPAPLVILLHGAGGDAEHGISLLR
                     GHAEHAGLLLLAVASRRSTWDVIQGGFGPDVEQLDRALAWTFARHAVDATRLAIAGFS
                     DGATYALSIGLGNGDLFTDIVAFSPGYMAPSRLVGRPRVFITHGVQDTVLPIDRCGRR
                     VAAALRSTGHDVTYKEFPDGHTVPAELRRAAVAWFDGDRESPSR"
     gene            complement(1857..3776)
                     /locus_tag="VGB85_13870"
     CDS             complement(1857..3776)
                     /locus_tag="VGB85_13870"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEY0135169.1"
                     /translation="MYRAIVLSLCLVLASSCGDSTGVASETGGTTGTRGTSSGDAEPT
                     STSQSTGGATSSTGDEPTPTTGAPPDCETSVGPGCSCGRGEVEIDGECVHETCAADTC
                     HGHGICSVTLIGVECKCDEGFVGERCETIGPDAYARTLLAPGLADPDIYKEHDDLFFL
                     TGTGNTVSVPIYETHDLLSFTEKVVYTPAALDPNHDYCYVWAPDLTKHEGVYHLYFSA
                     HQVAKGAACPPGPGQDVTTFHATAPSLDLAFGPPELVHDQTNLPRSRVASGCVAEGCS
                     RTIRIDAAAFDDGAERWLFYVWFQGGNNIAGFRASAPEAVVINAGPAVFPIEAFEEKI
                     NEGPDVFARGGKYHLFFSTGFYDSQYAMFHIMADSVGELTRARAVRRHSTPLRAANNN
                     LVETHGHNSIVERRGEFFNIFHMGSFDGGGKLVDRSTHKQRIAFKPDGSIHALNYVDL
                     RWSAAPGHKYSLDVVRRDGTVVGPCIAAGLLGSATSTRYTGICPSAGDLRVPKSDIAE
                     FRLYYSNDDQWTQHVDEPYDGFRDQVFLAVPGGATQAVEVAWNERATGAEYSLDVLRE
                     DNTWVAPCAGENVLGSGIRYTFNGSCPTANTSVSLQDIKELRVCSAVGDDWANAVCGS
                     VPYDGVAGFIDVAIP"
     gene            complement(3798..>4642)
                     /locus_tag="VGB85_13875"
     CDS             complement(3798..>4642)
                     /locus_tag="VGB85_13875"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HEY0135170.1"
                     /translation="LILHGADFDLRLLRRTRGFIASEVYDTAIAAQLLGRPRHGLRDL
                     VRELLGVELQKGSQRADWARRPLSQQMLAYAVNDTRYLSPLRDQLDAELHSKGRASWH
                     RETCARLVVTCSVPTTPDLERAWRIKGSATLDPRGLAVLRELWRWREAQALRLDRPPY
                     FVLSHEVLLELAGRAARGDMTLPPVIKARDRRELEEALARALDLPGEALPAREAPGRP
                     PRIANEVLRRMEAIRARRDRVAAELEIDPSLIATKSDIAALAEDFERGAAALMTWQAA
                     LLAP"
BASE COUNT          691 a         1667 c         1582 g          702 t
ORIGIN      
        1 tggtgataat cgccggggcg cgtcaggccg agcgtcgcca gcatcatcac gccgacgccc
       61 gcgccgaaga agccgccata caccgcggtc gcgccctgca ccacgagccc gaccgccggg
      121 cctggcgccg cctgcccctc ccgcggcctc ggcttcaacc acggcccggc cgcgaacagc
      181 agcgtggcgg cgagcagcag ccacggcacc agcgcacgaa acgacgcgtt gtcgagcgcg
      241 agcagcagcc acgagccgag cgtcgcgccg acgatcgagg cgacacacag cgccagcgcg
      301 gcgcgccact gctgctggat ctccgcgcgg taggccagcg ccgaggcgat gtagcccggg
      361 aattgcgtca gcgacgaggt cgtattggcg aggatcggcg acaccccgat cacggtcagc
      421 gcgccgaagg ttatgaatgt tccgccgccc gcgaccgcgt tgacgacccc ggcgaggaac
      481 cccgcgacaa agatcgtgat ggcgatcgtg agctgcaccg gccgcgaaag accggttatc
      541 gctgtccaga cttctcgccc tgccccggtt cgggcagctc gctgccgcgg gtgcccgggc
      601 ccgcgctgtc gtcctctgcg taattgctgt cctgatcctt gccgtcctgg ccggcgcgcg
      661 acagcccgcc ctttgtatcc tcgtccgcgc gatcgggggt ctttgggttc gccatgctcc
      721 cagtctgcgc gaccccgggc gcctgtcgaa cgccaggggc gctcgcccgt cggcgccgac
      781 gcctgcgcgc tcaccactga cacaggttcc atgcccagcc ggcacgcgcc gcggccagcg
      841 ccggcgaatg gccacagttg caccggagtt ttcccaacta cgcgggcgcg cggagtcggt
      901 cgatactcgc gctctgcata gatccgtata ctcgcgtcgc ggactgctgt ggacggctgg
      961 cagcatcgga ctgtgggccg cgtgccgggg cccatcgcca cccttgcagg atgctcgcga
     1021 cgaggacctg cccgacccgc gcccgaacga gagagacgtg atgaaccctt cgcctgcacc
     1081 cgcaccagga cctgatctcg ccgcagaggg ccggctcacg gcccgccccg gggctccggg
     1141 cgcgcccggc gacgccgcat ccgcgggcac gtaccgcctc gggctcacga ccacgcgtga
     1201 cggcctgatc cacgtgcctg cggcgatcga cgccgggaag cctgccccgc tggtcatcct
     1261 gctgcacggg gccggcggcg acgcggagca cggcatctcg ctattgcgtg gccacgccga
     1321 gcacgccggc ctgctgctgc tcgccgtcgc gtcgcgtcgc tcgacctggg acgtcattca
     1381 gggcggcttc ggccccgacg tcgagcagct cgaccgggcg ctggcctgga ccttcgcgcg
     1441 ccacgcggtc gacgcgacgc gcctggcgat cgccggattc tcggacggtg ccacctatgc
     1501 gctgtcgatc ggcctcggca acggcgacct gttcaccgat atcgtcgcct tttcgcccgg
     1561 atacatggca ccttcacggc tggtcggtcg gccccgcgtg ttcatcaccc acggcgttca
     1621 ggacaccgtg ctgccgatcg atcgctgcgg gcggcgggtg gccgccgcgc tgcgcagcac
     1681 cggccatgat gtcacctata aagaatttcc cgacggccac accgtcccgg ccgagctgcg
     1741 cagggccgcg gtcgcctggt tcgacggcga ccgcgaatcg ccgtcacgct agaaaaccca
     1801 gcagctcgcc acaggcgccg cggcagcggg tcgcggatct ctctcgcgtg caatattcag
     1861 gggatcgcaa catcgatgaa gcccgcgacg ccgtcgtagg ggacgctgcc gcacaccgcg
     1921 ttggcccagt cgtcgccgac cgccgagcac acgcgcagct ccttgatatc ctgcaacgac
     1981 acgctggtgt tcgcggtcgg acagctgcca ttgaaggtgt agcgaatgcc gctgccgagc
     2041 acgttctccc cggcgcaggg ggcgacccag gtgttgtcct cgcgcaggac atcgagcgag
     2101 tactcggcgc cggtggcccg ctcgttccac gcgacctcga ccgcctgcgt cgcgccgccc
     2161 gggaccgcca ggaacacctg gtcgcgaaag ccgtcgtacg gctcgtcgac gtgctgggtc
     2221 cactggtcgt cattcgagta atacaggcga aactccgcga tgtcggactt tggcacccgc
     2281 aggtcgccgg cgctcgggca aatgcccgta tagcgcgtcg aggtcgcgct gccgagcagc
     2341 cccgcggcga tgcacggacc gacgacggtg ccgtcgcgcc gcacgacatc gagcgaatac
     2401 ttgtgccccg gcgccgcgct ccagcgcagg tcgacatagt tgagcgcgtg gatcgaccca
     2461 tccggcttga acgcgatccg ctgcttgtgc gtgctgcggt cgacgagctt gccgccgccg
     2521 tcgaacgagc ccatgtggaa gatattgaaa aattcaccgc gccgctcgac gatcgagttg
     2581 tggccgtggg tctccaccag gttattgttg gcggcgcgca gcggcgtcga gtggcggcgc
     2641 accgcgcggg cccgcgtcag ctcgccgacc gagtcggcca tgatgtggaa catcgcgtac
     2701 tggctgtcgt agaacccggt gctgaagaac aggtgatatt tgccgccgcg cgcgaatacg
     2761 tccggcccct cgttgatctt ctcctcgaag gcttcgatcg ggaacaccgc cgggccggcg
     2821 ttgatcacca ccgcctcggg cgcggacgcg cgaaacccgg cgatgttgtt gccgccctgg
     2881 aaccacacat aaaacagcca gcgctcggcg ccgtcgtcga acgccgcggc gtcgatccgg
     2941 atcgtgcggc tgcagccctc ggccacgcac ccggaggcga cgcggctgcg cggcaggttg
     3001 gtctgatcat ggacgagctc cggcggcccg aacgcgaggt cgaggctcgg cgcggtcgcg
     3061 tgaaaggtcg tcacgtcctg gccaggaccc ggcgggcagg cggccccctt tgcgacctga
     3121 tgcgccgaga agtacaaatg atacaccccc tcgtgctttg tcaggtccgg ggcccacacg
     3181 taacaataat cgtggttcgg gtcgagcgcg gcgggggtat agacgacctt ctccgtgaag
     3241 ctcaggaggt cgtgggtctc gtagatcggc acgctgaccg tgttgccggt gccggtcagg
     3301 aagaacaggt cgtcgtgctc tttataaatg tcggggtcgg cgaggccggg cgccaacaat
     3361 gtgcgcgcat atgcatcggg gccgatcgtc tcacagcgct cgcccacgaa gccctcgtcg
     3421 catttacact cgaccccgat gagcgtcacg gagcagatac cgtgcccgtg gcaggtgtcc
     3481 gcggcgcagg tctcgtgcac acactcgccg tcgatctcga cctcgccgcg gccgcacgaa
     3541 cagccgggcc cgacggaggt ctcgcagtcc ggcggcgccc ccgtggtcgg ggtcggctcg
     3601 tcaccggtcg agctcgtggc gccgccggtc gactgcgagg tgctcgtcgg ctccgcgtcg
     3661 ccgctggacg tgccgcgcgt ccccgtcgtg ccgccggtct cgctcgccac gccggtgctg
     3721 tcaccgcagc tggaagcgag cacgaggcac agggacagga cgatcgcgcg atacatcgcc
     3781 gcgatgatac cggcggctca cggcgcgagc agcgcggcct gccaggtcat tagcgcggcg
     3841 gcgccccttt caaagtcctc ggcgagcgcc gcgatgtccg acttggtggc gatcagcgag
     3901 gggtcgatct ccagctcggc agccacccgg tcccggcggg cgcggatcgc ctccatgcgg
     3961 cgcagcactt cgttggcgat ccgcggcggg cgaccggggg cctcgcgggc cggcagtgcc
     4021 tcgccaggca agtcgagcgc acgcgccagc gcctcctcga gctcgcggcg gtcgcgggct
     4081 ttgataacag gcggcaaggt catatcacct cgcgccgcgc gacccgccag ctcgagcagg
     4141 acctcgtggc tgagcacgaa gtacggcggc cggtcgaggc gcagcgcctg ggcctcgcgc
     4201 cagcgccaca gctcgcgcag caccgcgagc ccgcgcgggt cgagcgtggc gctgcctttg
     4261 atccgccagg ctcgttcgag gtccggcgtc gtcgggacgg agcacgtgac gaccagccgc
     4321 gcgcaggtct cacggtgcca tgatgcccgg cccttgctgt gcagctccgc gtcgagctgg
     4381 tcgcgcagcg ggctgaggta gcgggtgtcg ttgaccgcgt acgcgagcat ttgctggctc
     4441 agcggccgcc gggcccagtc ggcccgctgc gagccttttt gcaattcgac gccgagcagc
     4501 tcgcgcacca ggtcacgcag cccgtgccgc ggccgcccga gcaattgcgc cgcgatcgcg
     4561 gtgtcgtaga cctcgctggc gatgaagccg cgggtgcgcc gcagcagccg cagatcgaag
     4621 tcggccccgt gcaggatcaa ct
//
LOCUS       DASWNJ010000820         6878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_473951, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000820 DASWNJ010000000
VERSION     DASWNJ010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6878
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473951"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VGB85_13880"
     CDS             <1..795
                     /locus_tag="VGB85_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135171.1"
                     /translation="LLYADATRCFFLEGDLYRGFLAHLRATALFQAQGNRHWHAVAML
                     LLGQFQQALGRPTVAAATLREARALALACGTHFYATIHHLHLGMWLASETDATSHAEA
                     RAIAETYVGRTDLGPAIAGASESILAAVAAHDGDLDAAASAAARAVASFVVMLPYRLV
                     ATALLVDILLQLGRVAEAREAAEDALRALAVEGGAGYGEVELHTAVLAARLADGDRDG
                     ARLAAQTALAALQRRIDAIDDPELRADYEALPGHRRLVALAAASRT"
     gene            901..2400
                     /locus_tag="VGB85_13885"
     CDS             901..2400
                     /locus_tag="VGB85_13885"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0135172.1"
                     /translation="MSIPGENTHLKLVTVGDFDPQSDAAIEYDFIGRVIAGRYTVKEQ
                     IGGGGMADVFRATDEQLGIDVVIKLLKPRMASDDLRARMVQEARAAAQVRHDNLVKVF
                     GTGMLDSTAYIVMELLPGPNLEKYLQEYRDQRIPWREALDLLLPALDALHAVHERGYV
                     HRDIKPGNLLITYVPGRSTQAVVIDLGLVKPDRALRCATSPPTTEVGRILCTPGYTSP
                     EQAAGLPVDRRSDVYSMGVTLYRVLAGRLPFHDARGQPVAVVLAKHIYNAPTRLAKAA
                     LGAHIPPAIAAVVESALCKLPTERPRTMLAFAEALRAAAASVPPDTERRMGIRALLLP
                     GCLGVVFAVLARALLFSAEVDPDPTATPEDPVPASIAVLPETPDTPLVTIELSEQAAL
                     LPAPVAPASPEPVPRRPSPLAAWHRALDARTPDVQRCADEHTGSVTRLAVAVTIDATG
                     QIAAHVADEPDNPLSRCIARRLASRPLTAPRKPTSLVHVFELQVTFPEK"
     gene            2397..3254
                     /locus_tag="VGB85_13890"
     CDS             2397..3254
                     /locus_tag="VGB85_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135173.1"
                     /translation="MTTSMNLHAAFILLLAPTSSVLSPQAAEYNARAMVFYDAGQLAP
                     AVDEFYAAYRAMPDAHRDRAGREQLLGSMRMTLLALHRETGEAAPLCRLQNILKEHVD
                     ALTVAYPNELDRIETRSARARHKEVTAQLAAIGPDACRPPRVAPPVVAPATPPPVAAP
                     TPQVPLADPRTRNQLIAGGVMLPLGVVALAVTGVLAYHHRRHLAEFETTTAGTQPLTD
                     EDCTRLRTLRTATRREEGLMFALGITGGALVATGTALLIRGAMSPRRARLGLDLRRDR
                     VGLTITGEF"
     gene            3260..4144
                     /locus_tag="VGB85_13895"
     CDS             3260..4144
                     /locus_tag="VGB85_13895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135174.1"
                     /translation="MRSSSIICIVTMLAVLPACGSQRISDYLAEHCSLCSSTTDETGT
                     TAAPGEDTSSSGSDETTLESPETTGTGTTEASTSTPEETGTMQGSTGEPTGECGNGVL
                     EPFGLEPEECDDGNLDPLDGCSDTCALDRGAFVTSVLYNGAGIESLKIADALCFNRAD
                     DQGLSDALKYRAWLSNSTTDARDRFKRGRGRLVMMNGLVLAASWDALLSGALENNFEV
                     TEKSETYHGGVWTGTQPDGTAVPGSQHCDDWSNGWFMTTGHYGYSDRTTPEWTFAQDS
                     DQPSPCNSDYALYCIQSL"
     gene            4171..5271
                     /locus_tag="VGB85_13900"
     CDS             4171..5271
                     /locus_tag="VGB85_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135175.1"
                     /translation="MRLQRYIFSLLFPLSTPACSHSGGPDLETSVGTTTTGEPATSDV
                     PVPTEASGDEPTTTGATYTGATDDDTDTSGSPADTTTDGSSPCGDGIVDPGEQCDDGV
                     GNANHLFCTENCTINVCGDGKLLVNWEICDEGPANSDQWGSLCGTSCVPAPRCGDGKL
                     QPEFESCDLGPDNGGTKGDEQGILCDGTCKAQRLRGFVTSAAFTGDLGGLFGADLKCQ
                     DAAAAAGLPEPKRFYALLSTGDIDARTRFKAVAPSMPYVLISGKKFADNFTALIEAGP
                     LDEGISITETGAALFTKNVATNTAPGGDRFSPDQHCQGWTSADPAYQARAGVNAVPVD
                     APQWPEWQKTQWWISALGRQCSKEFHLYCLEI"
     gene            5465..6409
                     /locus_tag="VGB85_13905"
     CDS             5465..6409
                     /locus_tag="VGB85_13905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0135176.1"
                     /translation="MRVELRDLGKTYDGERWAVRGLTLTIPSGSIVGLIGPNGAGKST
                     TLRMLSTVLGPSEGSITFDGKPPGADKSALRRQIGFLGDGNPLYKHMSPAEYLRFFGQ
                     CFALPEATLEPAIEDMLTTFDLRDKRDTPSGALSKGMRQRLLIARCLLHGPRLLILDE
                     PADGLDPRGRSDLRTILSQTRARGVTVLISSHILRELDDLCDQMAILQQGKLVVAGDV
                     ADIIERYDVRRFVYQLRLLGGAPAAREVLARRRALIEAEGFEDGCETMSIQVQGGEEL
                     MADILADLVGAGVRVVTVSRVRSRLEDVYNRLSEDRVA"
     gene            6462..>6878
                     /locus_tag="VGB85_13910"
     CDS             6462..>6878
                     /locus_tag="VGB85_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135177.1"
                     /translation="MSFTRKLRAASLPFVTRALRGGRKLRYTGAAALLALLSIVIGCW
                     LALGSGSLEHDLRVGFKLERPMYMQEHQEFKIFASNPQELANLQAAVRKLTRSENLRA
                     IAESELYGSTEEEIRVYERVRQGLDLVTRVQHPHIFN"
BASE COUNT         1090 a         2531 c         2278 g          979 t
ORIGIN      
        1 ctgctgtacg ccgacgcgac gcgctgcttc ttcctggaag gcgacctgta ccgcggcttc
       61 ctcgcgcatc tgcgcgcgac cgcgctgttc caggcgcagg gaaatcgtca ctggcacgcc
      121 gtggcgatgc tgctgctcgg ccagtttcag caggcgctcg ggcgccccac ggtcgccgcc
      181 gcgacgctac gcgaggcccg ggcgctcgcg ctcgcgtgcg ggacacactt ctacgcgacg
      241 atccaccacc tgcacctcgg catgtggctc gcaagcgaga ccgacgccac cagccacgcc
      301 gaggcccgcg cgatcgccga gacatacgtc gggcggaccg acctcggccc cgcgatcgcg
      361 ggcgcgtcgg agtcgatcct cgcggccgtc gcggcgcatg acggtgacct cgacgccgcc
      421 gcctccgcgg ccgcacgcgc cgtcgcgtcg ttcgtggtga tgttgcccta ccggctcgtg
      481 gccaccgcgc tgctcgtcga catcttgctg cagctcgggc gcgtcgccga ggcccgcgag
      541 gccgcggagg acgccctgcg cgcgctcgcg gtcgagggcg gcgccggcta cggcgaggtc
      601 gagctccaca ccgccgtgct cgcggcccgc ctcgccgacg gcgaccgtga tggcgcccgc
      661 ctcgcggcgc agacggccct cgccgccctg caacgacgta tcgacgccat cgacgacccg
      721 gagctgcgcg ccgattacga ggcgttgccc gggcatcgcc ggctggtcgc gctcgcggcg
      781 gcctcgcgga cctgagcgag ccggcgcggg ttctccggcc aaaggcgcac tggacagccc
      841 gccacgggcc ccgatacagt gcctcgcgcg atctcgcgcg tgcccatgga ggggccaccg
      901 atgtcgatcc ccggcgagaa cacccacctc aagctcgtca ccgtcgggga cttcgacccg
      961 cagtccgacg cggcgatcga atacgacttc atcggccgcg tgatcgccgg gcgttatacc
     1021 gtcaaggagc agatcggcgg tggtggcatg gccgacgtgt ttcgcgccac cgatgagcag
     1081 ctcggcatcg acgtcgtcat caagctgcta aagcccagga tggcctccga cgacctgcgc
     1141 gcgcgcatgg tccaggaggc ccgcgccgcc gcccaggtcc gccacgacaa cctcgtgaag
     1201 gtgttcggca ccggcatgct cgacagcacc gcgtacatcg tgatggagct gctgccgggc
     1261 ccgaacctcg agaagtacct ccaggaatat cgcgatcagc ggatcccgtg gcgtgaggcg
     1321 ctcgacctgc tgctgcccgc gctcgacgcc ctgcacgccg tccacgagcg gggttatgtc
     1381 caccgcgaca tcaagcccgg caacctgctg atcacctatg tgcccgggcg ctcgacgcag
     1441 gcggtcgtga tcgacctcgg cctcgtcaag ccggaccggg cgctgcgctg cgcgaccagc
     1501 ccgccgacca ccgaggtcgg ccgcatcctg tgcacccccg gatatacctc gcccgagcag
     1561 gccgcgggcc tgcccgtcga ccgccggtcc gacgtctact cgatgggcgt cacgctctac
     1621 cgcgtgctcg ccgggcggct gccgttccac gacgcgcgcg gccagccggt cgcggtcgtg
     1681 ctcgccaaac atatctataa tgcgccgacc cggctggcga aggcggccct cggcgcacat
     1741 attccgccgg cgatcgcggc ggtggtcgag tccgcgttat gcaagctccc gaccgagcgg
     1801 ccgcggacga tgctcgcgtt cgcggaggcg ctgcgagccg cggccgccag cgtgcccccg
     1861 gacaccgagc gccgcatggg gatccgcgcg ctgctgctcc cgggctgtct cggcgtcgtc
     1921 tttgctgtgc tggcgagggc cctgctgttc tctgctgaag tcgatcccga cccgacggcg
     1981 acgcccgagg atccggtccc cgcgagcatc gcggtgctgc cggagacgcc ggatacccct
     2041 ctggtgacca tcgagctttc ggagcaggcc gcgctgctcc ccgcgccggt cgcacccgcg
     2101 tccccggagc cggtcccgcg gcgcccctcg ccgctcgccg cgtggcaccg ggccctcgat
     2161 gcgcgcacgc ccgatgtcca gcgctgcgcc gacgagcaca ccggcagcgt cacgcgcctc
     2221 gcggtcgccg tgaccatcga cgcgaccggg cagatcgccg cccacgtcgc ggacgagccc
     2281 gataatcccc tcagtcgctg catcgcccgg cgcctcgcgt cgaggcccct caccgcccca
     2341 cgcaagccca cgtccctcgt gcacgtcttc gagctgcagg tgacattccc cgagaaatga
     2401 cgacatcgat gaacctccac gccgccttta tcctgctcct cgcgccgacc tcgagcgtgc
     2461 tgtcaccgca ggccgccgag tacaacgccc gggcgatggt cttttatgac gccggccagc
     2521 tggcgcccgc ggtcgacgag ttttacgcgg cgtaccgcgc gatgccggac gcacatcgcg
     2581 accgcgccgg ccgcgagcag ctgctcggct cgatgcgcat gacgctgctc gcgctgcacc
     2641 gcgagaccgg cgaggccgcg cccctgtgcc ggttacagaa catcttgaaa gagcacgtcg
     2701 atgcgctgac ggtcgcgtat ccaaacgagc tcgacaggat cgagacccgc agcgcccgcg
     2761 cgcggcataa ggaggtgacg gcccagctcg ccgcgatcgg ccccgacgcg tgccgaccac
     2821 cgcgcgtggc cccacccgtg gtcgccccgg cgacgccccc gcccgtcgcg gccccgaccc
     2881 cgcaggtccc gctcgccgac ccgcgcacgc gcaaccagct gatcgccggc ggagtgatgc
     2941 tcccgctcgg cgtcgtcgcg ctggccgtca ccggagtgct cgcataccac caccggcgcc
     3001 acctcgccga gttcgagacg acgaccgcgg ggacgcaacc cctcaccgac gaggactgca
     3061 cccgcctgcg cacgctgagg accgcgacgc gacgcgagga ggggctgatg ttcgcgctcg
     3121 ggatcacggg cggggcgctg gtggccaccg gtactgcgct gctgatccgc ggcgcgatga
     3181 gccctcgacg ggcgcgactg ggcctcgacc tccggcgcga tcgcgtcggc ctcacgatca
     3241 caggagaatt ttaaaagtca tgcgcagctc atccatcatc tgcatcgtca ccatgctcgc
     3301 tgtcctcccc gcatgcggct cgcagcggat ctcggactac ctcgccgagc actgctcgct
     3361 gtgttccagc accaccgacg agaccggaac cacggcggcg cccggcgagg acaccagctc
     3421 gtcggggagc gacgagacga cgctcgagag cccggagacg accggcaccg gcacgacgga
     3481 ggccagcacg tccaccccgg aggagaccgg caccatgcag ggcagcaccg gcgagccgac
     3541 gggcgagtgc gggaacgggg tgctcgagcc cttcggcctg gagcccgagg agtgcgacga
     3601 cgggaacctc gacccgctcg acggctgcag cgacacgtgc gcgctcgacc ggggggcgtt
     3661 cgtgacgagc gtgctgtaca acggcgccgg gatcgagagc ctgaagatcg ccgatgcgct
     3721 ctgcttcaac cgggccgacg accagggcct gtccgacgcg ctcaagtatc gggcctggct
     3781 cagcaacagc acgaccgacg cccgcgaccg gttcaagcgc ggccgcgggc ggctggtaat
     3841 gatgaacggg ctggtgctcg ccgcctcgtg ggacgcgctg ctgagcggcg ccctggagaa
     3901 caacttcgag gtgacggaaa agagcgagac ttaccacggc ggggtctgga ccgggacaca
     3961 gccggacggg accgccgtgc ccgggtcgca acactgcgac gattggtcga acggctggtt
     4021 catgacgaca ggccactacg gctacagcga tcgcacgacg ccggagtgga ccttcgcgca
     4081 agactccgat cagcccagcc cgtgtaactc ggattatgcc ctctactgca tccaaagcct
     4141 ctgaccctcg aacaggagat cttcatgtcc atgcgcctgc aacggtatat tttcagcctg
     4201 ctcttcccgc tcagcacccc cgcgtgcagt cattcaggag gaccagacct cgagaccagc
     4261 gtcgggacca ccacgaccgg cgaaccggcg acctccgatg tcccggtgcc cacggaagcc
     4321 agcggtgacg aaccgaccac gactggcgcc acgtacacag gtgccaccga cgacgatacg
     4381 gatacgagcg gctcgccagc ggataccacg accgacggga gctcgccgtg cggagacggt
     4441 atcgtcgatc caggggagca gtgtgacgac ggcgtcggca atgccaacca tctgttttgc
     4501 accgaaaact gtaccatcaa cgtatgtgga gacggcaagc tgctcgtgaa ctgggaaatt
     4561 tgcgacgaag gcccggcgaa tagtgatcaa tggggctctc tctgcggcac gagctgtgtt
     4621 ccggctccgc gctgcggaga cggaaagttg caacccgagt tcgagagctg cgacctcggc
     4681 ccggacaacg gaggaaccaa gggagatgag caggggatcc tgtgtgacgg gacctgcaag
     4741 gcgcagcggc tgcgaggctt cgtcacgtcg gcggcgttca ccggcgatct cggcgggttg
     4801 ttcggggcgg acctcaagtg ccaggacgca gccgccgccg ccgggttgcc cgagccgaag
     4861 cgtttctacg cgctgctgag caccggagac atcgacgcga ggacgcgctt caaggcggtc
     4921 gctccctcga tgccctacgt gctgatctcg ggcaagaagt tcgcggacaa cttcacggcg
     4981 ttgatcgaag ccggaccgct cgacgagggc atctcgatca cagagacggg cgccgcgctg
     5041 ttcaccaaga acgtggcgac caacacggcg cccgggggcg atcgcttcag cccggatcaa
     5101 cactgccagg gctggaccag cgccgacccg gcgtaccagg cccgggccgg cgtcaacgcc
     5161 gtcccggtcg atgcgccgca gtggccagag tggcagaaaa cccagtggtg gatcagcgcc
     5221 cttggtcggc aatgcagcaa ggagttccac ctctactgcc tcgagatttg acgccgcggg
     5281 ccctcacgag cccgtgatcc cggtcggcgg tgccagtcgg cgaggccgcg ggcgtaccgc
     5341 cggtcgaagc gccggaggtg tcgccgccgc ccccgacgat caccaggata tgccccagga
     5401 gcgcgatccg gcgccttcca gccgctggcg gaataaccgc gaatcgcgct acgttgccag
     5461 gctgatgcgt gtcgagctgc gagacctcgg gaagacctac gacggcgagc gctgggccgt
     5521 ccgcgggctc acgctgacga tccccagcgg ctcgatcgtc ggcctgatcg ggcccaacgg
     5581 ggccggcaag tcgacgaccc tgcgcatgct gtcgaccgtg ctcggcccga gcgagggcag
     5641 catcaccttc gacggcaagc cgcccggggc cgacaagagc gcgctgcgcc ggcagatcgg
     5701 gtttttgggc gacggcaacc cgctctacaa gcacatgtcg ccggccgagt atctgcggtt
     5761 cttcggccag tgcttcgcgt tgcccgaggc gaccctcgag ccggcgatcg aggacatgct
     5821 gacgaccttc gacctgcgcg acaagcggga caccccgagc ggcgcgctgt cgaagggcat
     5881 gcgccagcgc ctgctgatcg cccggtgtct gttgcacggc ccgcgcctgc tgatcctcga
     5941 cgagccggcc gacgggctcg acccgcgcgg tcgcagcgac ctgcgaacga tcctctcgca
     6001 gacgcgcgcg cgcggcgtca ccgtgctgat ctcctcgcac atcctgcgcg agctcgacga
     6061 cctctgcgat cagatggcga tccttcagca gggcaagctc gtggtcgccg gcgacgtcgc
     6121 cgacatcatc gagcgctacg acgtccgccg cttcgtctac cagctgcgcc tgctcggcgg
     6181 cgcgcccgct gcccgcgagg tgctcgcccg ccgccgcgcc ttgatcgagg ccgagggctt
     6241 cgaggacggc tgcgagacca tgtccatcca ggtccagggc ggcgaggagc tgatggccga
     6301 catcctcgcc gacctcgtcg gggcgggcgt tcgcgtcgtc accgtcagcc gggtacgcag
     6361 ccgcctcgag gacgtctaca atcgcctctc cgaggaccgc gtggcctaag gtgccacact
     6421 ccgggaactc ctgtccgccg cctctgtccg aaggatctgc catgtcgttc actcgcaagc
     6481 ttcgtgccgc ctcgctgcct ttcgtcactc gcgccctgcg gggcggtcgc aagctccgct
     6541 acaccggagc cgcggcgctg ctcgccctgc tgtcgatcgt gatcggctgc tggctcgcgc
     6601 tcggctccgg atcgctcgag cacgacctgc gcgtcggctt caagctcgag cggccgatgt
     6661 acatgcagga gcatcaggag ttcaagatct tcgccagcaa cccccaggag ctcgcgaacc
     6721 tgcaggccgc cgtccgcaag ctgacccgca gcgagaacct gcgcgcgatc gccgagtccg
     6781 agctctacgg gtccaccgag gaggagatcc gcgtgtacga gcgcgtgcgg cagggcctcg
     6841 acctcgtgac ccgcgtccag cacccgcaca tcttcaac
//
LOCUS       DASWNJ010000821         9140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_156442, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000821 DASWNJ010000000
VERSION     DASWNJ010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 9140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9140
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156442"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..930
                     /locus_tag="VGB85_13915"
     CDS             139..930
                     /locus_tag="VGB85_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135178.1"
                     /translation="MNTKALVFALAAACFSAAVPAIAQDYGRDDQRRYEQRDGRHDGD
                     SRGQRGDERGRYAHDRNDRNDRDARDRDRDRASRSGTFNVSAFADKEDGLVALGRSRS
                     PSTPHTQAPSQPPARERGVFSGFNSTPVPDMPRSEPSRAEPRREPAAPETRPRASSSS
                     SSSSSTTSSSLLASFMALAEQAQQPQPPEAKRPEPKPPAPPKTIPPSPSPRPEAQARP
                     PVKQPPPPPAPAKPAPPAPLPEPTYKVEEPGNITPALSPDDPDYF"
     gene            940..1236
                     /locus_tag="VGB85_13920"
     CDS             940..1236
                     /locus_tag="VGB85_13920"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY0135179.1"
                     /translation="MPKIAPLTSTERSEGLARIPEWKPLADRDAIHRSIRFADFNAAW
                     GFMSRVALIAEQMEHHPEWFNVYNRVDITLTTHDAGGLSQRDLDLAAAIDRLTP"
     gene            1242..1763
                     /locus_tag="VGB85_13925"
     CDS             1242..1763
                     /locus_tag="VGB85_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135180.1"
                     /translation="MRSLTLALLLACACDGPTSPPPATPPAPAAQPAPAPAPATPPAA
                     PATPPATAPKTPPKTPSATPPKTPPDEGDDNPGFADPKTAAGDEPAPATPPKTAEDPA
                     LAAKITKQFGERCKLERSCGDLLGVDCQSAVDGPYYYVQRRDLKTVATCGGACRRTGC
                     TDCPPKAWTCPTY"
     gene            1794..2642
                     /locus_tag="VGB85_13930"
     CDS             1794..2642
                     /locus_tag="VGB85_13930"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0135181.1"
                     /translation="MLLPLAERLIRRRLHALGITSGQLTTASGDLHYYQAGGARDLPP
                     LVMLHGFGANATSFARVLAHLHTRIGPIIAPDAPAHGFSTTRHRPMTVDLAYAGVAEL
                     LLRRTDTPAILFGNSMGGAFALRFALEHPERVRGLFLSSPVGAALDAQDFSSLRDSFR
                     VRSLGHARRLVDSLTHRQAWFTPIFTPDTLWRFGRGPLRAVLESFQPAHMFTAEQLAD
                     LRAPTLLQWGRSERVLPPASLEFFRRALPPHVVFEEPDEFGHCPHMDQPRQVAARIAD
                     FVRSLA"
     gene            complement(2688..4034)
                     /locus_tag="VGB85_13935"
     CDS             complement(2688..4034)
                     /locus_tag="VGB85_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135182.1"
                     /translation="MSTFPYSLLLVPLLGACTIQKDLLATGAPDSTDDGAASEPAVTT
                     GEMTATDDPTATGDPTGAIDPMNTTGAELDTSTGDSFATTHDTEVDTEGCDTACLDEP
                     SPCVEALAWDDGLIGMTARRVTNCAECGLHETLETLLVRIDVGSNVMTVFAEAGIRSD
                     GNAMVIAGDGAIFVGGHRSAIVEGDVEWQQLHKFSPEGALLWTLEPGGSTISDLALRG
                     DELFIAGRFGELAGRAVTDGALLWDIPQELGVSLTGIEVDGDGAFYVVGDTDFGPDQN
                     PLHTMFIRKYAADHTLLWEDVHTPPLPTDYISAEGLALDEAGGVVIATREYVLGPGLD
                     VTTFRKYDAAGALLWAETYPEIGTGDALLQLRARPGGGVIAVGRGGSEGGGLGGFTVA
                     LGPDGDVSWSDRKAGPQLKDQRNNDVVAVDDTLLVTGCAYHEVTVEQSSWLLELTP"
     gene            4183..4368
                     /locus_tag="VGB85_13940"
     CDS             4183..4368
                     /locus_tag="VGB85_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135183.1"
                     /translation="MPRHVELFHAPVMSRTSRMATQRDVRSWPFRAAGRVYVTSFDGP
                     HHEEMRAHLAVDARAAW"
     gene            4411..5781
                     /locus_tag="VGB85_13945"
     CDS             4411..5781
                     /locus_tag="VGB85_13945"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0135184.1"
                     /translation="MAHRSLELTFLGGTGTITGARYLLDSGECGVLVDCGVYQGFKNL
                     RRRNRDPLPVRRLDAVVLTHAHPDHAGHLPRLVRDGYRGPIYCTPGTAALCGILLPDS
                     AATQLQDAAFANKKGYTKHHPAEPLYSAEDVARTLGMLRPIEFGERFALPDNLHGQFT
                     RSGHILGAASLRVDDGTRRVLFSGDLGREQDVMMAPPEPRPQADVVILESTYGGRQHD
                     GEDPGEVVAELIRRAVTRGGTVLIPAFCVGRTQALLVFLYQLRRAKKLPDVPIFVDSP
                     MASSAAEIHAAHPKDHRLSREMCEAAFGIAEYVRDVDDSHALDKRSEPAIIIASAGMA
                     TAGRILHHLKRYAPDPNSAIVLAGYQAAGTRGGTLATGADTLKIHGRHVQVKAEVAHL
                     DMLSAHADQEELLGWLEHGDAPRHTYLVHGEPAAADALRHTIEEQLRWPVSVPDYRDT
                     IDIHAL"
     gene            complement(5789..6994)
                     /locus_tag="VGB85_13950"
     CDS             complement(5789..6994)
                     /locus_tag="VGB85_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135185.1"
                     /translation="MHGLLIAASLLATPTAAAAGAPEAVVGQRPVARVWLRGEGLDMT
                     DLAAAVQARLGDKEILGPGASAWASAGLAALCLIRWDARAGVVRLEVILGDGRVYERS
                     IAAKAAGRERTAARLLASTLAAIEDETATPDRHDGVFDAPDVAREEVPEIDAPEPDDV
                     STVPAPPSAPSVPVQDELEVAEPSARQPVQAPVGRLEVAEPESPARKPVQAPVGRLEL
                     GVAIDPGVVLGLRSPGRGLAGAGGGLRVDLRLRRGVMFGAGFRGLAHTRDALVLGRFR
                     GALVAGYAYRRGALELAALGGPTLETWQVTQRGAPVAYEASGPGGASLLLGGLARLVA
                     GARVTRGKTAALRVGGYVELAGSSRTSGRAAQVARLDAMGTPVTVFVLGGAELSLGLE
                     LELWLALRR"
     gene            complement(7045..8301)
                     /locus_tag="VGB85_13955"
     CDS             complement(7045..8301)
                     /locus_tag="VGB85_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135186.1"
                     /translation="MTTTRILRLLSPFLFTTLGVVGTLGNAGCPLPKQIDEDDEGDCD
                     DSGAEQGQEAGDTCGPNDPDCLGATDEGGCDPNTPDCGDGICDPMNMADPDCPVCAPG
                     DPNCGCDPNTPNCSDGVCDPMNVGDPDCKCPGDDPGCPDNVCDPMQADDPDCCAPDDP
                     NCSDGVCSATPGDPDCVCQDPNDPSCGDGVCDPMLPGDPDCNCPDNDPNCGDGVCDPM
                     LPGDPDCADLCVADSDCQAGQVCVNGGCVCADPNDPTCGDGVCNPMLPEDVDCCAPDD
                     PMCSDGFCDANLPGDPDCACPPGDPTCPNACDAKFPLDPDCVLSCMADSDCAPNQVCS
                     DNLCTCIDSNDPTCSDTDCDPMAPNDPDCCDPNDANCSDDICDADAPADPDCAPLECA
                     ADSDCLMGQVCINGGCSCADPNDPDC"
     gene            complement(8298..8486)
                     /locus_tag="VGB85_13960"
     CDS             complement(8298..8486)
                     /locus_tag="VGB85_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135187.1"
                     /translation="MLLFGCDTDDFVIAGETDGCSSSGGAEEHALDEPVIDPPLAERQ
                     HDDLELSRPTRAPVQERR"
     gene            complement(8513..>9140)
                     /locus_tag="VGB85_13965"
     CDS             complement(8513..>9140)
                     /locus_tag="VGB85_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135188.1"
                     /translation="PGSLAGLLGAVADRRAAVEAARERPPAPAVTCPEGQVCTDGAAG
                     QTCPAGQACVAQCTCPAGQPGCAPGCGSRPEAPVVRRRPRAGEDLATRLRRLDDAAEA
                     LLGAGDLAGADERYVEIAAIGGTRPEVEHAFADRFGLARTRRDTASQRRLWRAYLERF
                     PRGGFADDAHAGLCRTAPDDEHGACWARYLENFPAGAHRREAGEQESP"
BASE COUNT         1287 a         3629 c         2995 g         1229 t
ORIGIN      
        1 aacactgtag gtcggccgtg cgattcgagg tctaatgtct ctcatggaag cacggtgttg
       61 tgccctgtcc ctggaaagag cggaactctt acaggactca acgagacggc agcgtctcac
      121 ttgattactg gagttgatat gaacacgaaa gccctggtgt ttgcccttgc cgcagcttgc
      181 tttagcgcgg ccgtccccgc gattgcccag gactatggac gtgacgacca gcgccgctac
      241 gagcagcgcg acggccggca tgacggcgac agccggggcc agcgcggtga tgagcgcggc
      301 cgctatgccc atgaccgcaa cgaccgcaac gaccgcgacg cccgcgaccg cgaccgcgac
      361 cgcgccagcc gcagcggcac cttcaacgtc agcgccttcg cggacaagga ggacggcctg
      421 gtcgccctcg gccgctcgcg cagcccgagc acgccgcaca cccaggcccc gtcgcagccg
      481 cccgcgcgcg agcgcggcgt ctttagcggc ttcaacagca cgccggtgcc ggacatgccg
      541 cgctccgagc cgtcgcgcgc cgagccgcgc cgcgagcccg ccgcccccga gacgcgcccg
      601 cgggcctcgt cgtcctcatc atcctcatcc tcgaccacct cgtcctcgct gctcgccagc
      661 ttcatggcgc tcgccgagca ggcccagcag ccccagccgc ccgaggccaa gcgcccggag
      721 ccgaagcccc ccgcgccgcc gaagacgatc ccgccgagcc cgagcccgcg cccggaggcc
      781 caggcccgcc cgccggtcaa gcagccgccg ccgccgcccg cgcccgcgaa gccggccccg
      841 cccgcgccgc tgcccgagcc gacctacaag gtggaggagc ccggcaacat cacgccggcg
      901 ctgtcccccg acgacccgga ttacttctag gacaccgaca tgccgaagat cgccccgctc
      961 acgagcaccg aacgcagcga gggcctcgcc cgcatcccgg agtggaagcc gctcgccgac
     1021 cgcgacgcga tccaccgcag catccgcttc gccgacttca acgccgcctg gggcttcatg
     1081 tcccgcgtcg ccctgatcgc cgaacagatg gagcaccacc ccgagtggtt caacgtctac
     1141 aaccgcgtcg acatcacgct gacgacccac gacgccggcg gcctgagcca gcgcgacctc
     1201 gacctcgccg ccgcgatcga ccgcctcacc ccctgaccac catgcgatcc ctgaccctcg
     1261 ccctcctcct cgcctgcgcc tgcgacggcc ccacctcgcc cccgcccgcg accccgccgg
     1321 cccccgccgc ccagcccgcg ccggcccccg cacccgcgac gccacccgcc gcgcccgcga
     1381 caccgcccgc gaccgcgccg aagaccccgc cgaagacccc gtccgcgacc ccgccgaaga
     1441 ccccgccgga cgagggcgac gacaaccccg gcttcgccga cccgaagacc gccgccggcg
     1501 acgaacccgc gcccgcgacc ccgccgaaga ccgccgagga ccccgccctc gccgccaaga
     1561 tcacgaagca gttcggcgag cgctgcaagc tcgagcgcag ctgcggcgac ctgctcggcg
     1621 tcgactgtca gtcggccgtc gacggtccgt actattacgt ccagcgccgc gacctcaaga
     1681 ccgtcgcgac ttgcggaggc gcctgccgcc gcaccggctg caccgactgc cccccgaaag
     1741 cctggacctg cccgacgtac tagcatcgtc acctgtccct tcagacatcc gccatgctgc
     1801 tccccctggc cgaacgcctg atccgccgcc gcctccacgc tctcggcatc acgagcggcc
     1861 agctgacgac cgcctcgggg gaccttcact actaccaggc cggcggcgcc cgggacctgc
     1921 cgccgctggt gatgctgcac ggcttcggcg ccaacgcgac gtcctttgcc cgcgtgctcg
     1981 cccacctgca cacgcggatc ggcccgatca tcgcccccga cgcgccggcg cacggcttta
     2041 gcaccacgcg ccaccgcccg atgaccgtcg acctcgccta cgccggcgtc gccgagctgc
     2101 tgctgcgccg caccgacacc ccggcgatcc tgttcggcaa ctcgatgggc ggcgcgttcg
     2161 ccctgcgctt cgcgctcgag caccccgagc gcgtgcgcgg cctgttcctc tcgtctcccg
     2221 tcggcgccgc cctcgacgcc caggacttct cgagcctgcg cgactccttc cgcgtcagat
     2281 ctctcgggca cgcccggcgc ctcgtcgaca gcctcaccca ccgccaggcg tggttcaccc
     2341 cgatcttcac ccccgacacc ctgtggcgct tcggtcgggg cccgctgcgc gccgtgctcg
     2401 agtcgttcca gcccgcgcac atgttcaccg cggagcagct cgccgacctg cgcgcgccga
     2461 cactcctgca atggggccgc tccgagcgcg tgctgccccc cgcctcgctc gagttcttcc
     2521 gccgcgccct gccgccgcac gtggtcttcg aggagcccga cgagttcggc cactgcccgc
     2581 acatggacca gccccgccag gtcgccgccc gcatcgccga cttcgtgcgc tcgctggcgt
     2641 gacccgccgc ggcggcgcct gccggaccgc cgccgccggc catccgctca gggcgtcagc
     2701 tcgagcagcc agctcgactg ctcgaccgtc acttcgtggt aggcgcagcc ggtgaccagg
     2761 agggtgtcat cgacggcgac cacgtcgttg ttgcgctgat ctttgagctg gggacccgcc
     2821 ttgcgatccg accatgacac gtcaccgtcg gggccgagcg cgacggtgaa gccgccgagt
     2881 ccgccgccct ccgacccgcc ccggccgacc gcgatcaccc ccccgcctgg ccgggcccgc
     2941 agctgcagca gggcgtcgcc cgtgccgatc tcggggtagg tctcggccca cagcagggcg
     3001 cccgcggcgt cgtacttgcg aaaggtcgtc acgtcgagac cggggccgag cacatactcc
     3061 cgcgtggcga tgacgacccc gccggcctcg tcgagcgcga gaccctcggc gctgatgtaa
     3121 tcggtcggca gcggcggcgt gtgcacgtcc tcccacagca gcgtgtggtc cgccgcgtac
     3181 ttgcggatga acatcgtgtg caggggattc tggtcggggc cgaagtcggt gtcaccgacg
     3241 acgtagaagg cgccgtcgcc gtcgacctcg atcccggtca gggacacgcc cagctcctgg
     3301 gggatgtccc agagcagcgc cccgtccgtc acggcccggc ccgccagctc gccgaaccgg
     3361 cccgcgatga acagctcgtc gccacgcagg gcgaggtcgc tgatcgtgga gccgcccggc
     3421 tcgagggtcc acagcagcgc gccctcgggc gagaatttat gcagctgctg ccactcgacg
     3481 tctccctcga cgatcgcgct cctgtgaccc ccgacgaaga tcgcgccgtc cccggcgatc
     3541 accatggcgt tgccgtcgct gcggatcccg gcctcggcga acacggtcat cacgttgctg
     3601 ccgacgtcga tgcgcacgag cagcgtctcg agggtctcgt gcaagccaca ttccgcgcag
     3661 ttcgtgaccc ggcgggcggt catgccgatc aggccgtcgt cccacgcgag ggcctcgacg
     3721 cacgggctcg gctcgtccag gcaggccgtg tcacaaccct cggtgtcgac ctccgtgtcg
     3781 tgggtggtcg cgaagctatc acccgtggac gtgtccagct cggccccggt cgtgttcatg
     3841 gggtcgatgg cgccggtggg atcgccggtc gcggtgggat cgtcggtcgc ggtcatctcc
     3901 ccggtcgtga ccgccggctc gctcgccgcg ccgtcgtcgg tcgaatcggg agcgccggtg
     3961 gcgagcagat ccttctggat cgtgcacgcg ccgagcaagg ggacgagcaa tagagaataa
     4021 ggaaaggtgg acatgccccc tgcttctccg gccgtttgga ttcctgtctc ccgcagaaaa
     4081 atatttttac cgcgcgagcg cgccgccgtc gcggaggcga gaccgagcag cacatgtgcg
     4141 gcgcaccatc atccatccat cgcttcgagg ctctggccga ccatgcctcg ccacgtcgag
     4201 ctgttccatg cgccggtgat gtcaaggaca tcccgcatgg cgacgcagcg cgacgtgaga
     4261 tcctggccgt ttcgtgctgc cggacgagtg tacgtgacga gcttcgacgg gccgcaccac
     4321 gaggagatgc gcgcgcacct cgccgtggat gcacgcgccg cgtggtgagc acaccccggg
     4381 cgcgcgcccc tgtgctatcg ccgtcccccc atggcgcacc gctccctcga gctgaccttc
     4441 ctcggcggca ccggcacgat caccggcgcg cgatacctcc tcgacagcgg cgagtgtggc
     4501 gtgctcgtcg actgcggcgt gtaccagggc tttaaaaatt tacgccggcg taaccgcgac
     4561 ccgctgccgg tgcgccggct cgacgccgtc gtgctgaccc acgcgcaccc cgatcacgcc
     4621 ggccacctgc cgcgcctggt ccgtgacggc taccgcggcc cgatctactg cacccccggg
     4681 accgccgcgc tgtgtgggat cctgctgccc gacagcgccg ccacccagct gcaggacgcc
     4741 gccttcgcca acaagaaggg ctacacgaag caccacccgg ccgagccgct gtacagcgcc
     4801 gaggacgtcg cgcgcaccct ggggatgtta cggccgatcg agttcggcga gcgcttcgcc
     4861 ctgccggaca acctccacgg ccagttcacg cgctcgggcc acatcctcgg cgccgccagc
     4921 ctgcgcgtcg acgacggcac gcggcgcgtg ctgtttagcg gcgacctcgg gcgcgagcag
     4981 gacgtcatga tggcgccccc cgagccgcgc ccgcaggccg acgtcgtgat cctcgagtcg
     5041 acctacggcg gccgccagca cgacggagag gacccgggcg aggtcgtcgc cgagctgatc
     5101 cggcgcgccg tgacgcgcgg cggcaccgtg ctgatccccg cgttctgcgt cggccgcacg
     5161 caggcgctgc tggtgttcct gtaccagctg cgacgcgcca aaaaactgcc cgacgtcccg
     5221 atcttcgtcg acagcccgat ggcgagctcc gccgcggaga tccacgcggc gcacccgaag
     5281 gatcatcgcc tgtcgcgcga gatgtgcgag gcggccttcg ggatcgccga gtacgtgcgc
     5341 gatgtcgacg actcgcacgc gctcgacaag cgcagcgagc ccgcgatcat catcgcctcc
     5401 gccggcatgg cgaccgccgg gcggatcctc caccacctca agcgctacgc cccggaccca
     5461 aacagcgcga tcgtcctcgc cggctatcag gccgcgggca cccgcggcgg gaccctggcg
     5521 accggcgccg acacacttaa aattcacggc cgccacgtgc aggtcaaagc cgaggtcgcc
     5581 cacctcgaca tgctgtccgc ccacgccgac caggaggagc tgctcggctg gctcgagcac
     5641 ggcgacgctc cgcgccacac ctacctggtc cacggtgagc ccgccgccgc cgacgcgctg
     5701 cgccacacga tcgaggagca gctgcgctgg cccgtcagcg tgcccgacta ccgcgacacg
     5761 atcgacatcc acgcgctctg acgcccggtc accgtcgtaa tgcgagccac agctcgagct
     5821 cgaggcccag cgacagctcg gcgccgccga gcacgaacac cgtcaccggc gtccccatcg
     5881 cgtcgaggcg agccacctgc gcggcgcgtc cgctggtgcg gctgctgccc gcgagctcga
     5941 cgtacccgcc gacccgcagc gccgcggtct ttccccgcgt cacacgagcg ccggcgacca
     6001 gccgcgcgag cccgccgagc agcagcgacg cgccccccgg cccgctcgcc tcgtaggcca
     6061 ccggcgcgcc gcgttgcgtg acctgccagg tctcgagcgt cggaccgccg agggccgcca
     6121 gctcgagcgc gccgcggcgg tacgcgtaac cggcgacgag cgccccgcga aagcgcccga
     6181 gcaccagcgc gtcgcgtgta tgtgcgaggc cacgaaaccc ggccccgaac atcacgccgc
     6241 ggcgcaggcg aagatcgacc cgcagcccgc cgcccgcgcc cgcgagccca cgccccggac
     6301 ttcgcagccc gagcaccacc cccggatcga tcgccacgcc gagctcgagc ctaccgaccg
     6361 gcgcctgcac cggcttacgc gcaggagact cgggctccgc gacctcgagc ctaccgaccg
     6421 gcgcctgcac cggctgacgc gcagaaggct ccgcgacctc gagctcatcc tgcaccggca
     6481 cggacggcgc gctcggtggc gccggcaccg tcgacacatc atccggctcc ggcgcatcga
     6541 tctccggtac ctcttcacgc gcgacatcgg gcgcatcgaa caccccgtcg tgtcggtcgg
     6601 gcgtcgccgt ctcgtcctcg atcgcggcca gcgtcgacgc gagcagccgc gccgccgtgc
     6661 gctcgcggcc ggccgccttc gcggcgatgc tgcgctcgta cacgcgacca tcgccgagga
     6721 tcacctcgag ccgcaccacg cccgcccgcg cgtcccagcg gatcaggcac agcgcggcca
     6781 ggcccgcgct ggcccacgcc gacgcgccgg gcccgaggat ctccttgtcg ccgaggcgcg
     6841 cctgcacggc cgcggcgagg tccgtcatgt cgagcccctc gccgcgcagc cacacgcgcg
     6901 cgaccggccg ctgcccgacg acggcctcgg gggcccccgc ggccgcggcc gtcggggtcg
     6961 cgagcaggct cgcggcgatc aggagcccgt gcaaggccgc gagctttcca caggtcgcgc
     7021 tcccggacca gcctcgcacg cgcctcagca gtcggggtcg ttcgggtcgg cgcagctgca
     7081 cccgccgttg atgcagacct gtcccatcag acagtcgctg tccgccgcgc actcgagcgg
     7141 cgcgcagtcc gggtcggccg gcgcgtccgc gtcgcagatg tcgtcgctgc aattcgcgtc
     7201 gttcggatcg cagcagtccg ggtcgttcgg cgccatcggg tcgcagtcgg tgtcgctgca
     7261 ggtcgggtcg ttggagtcga tgcaggtgca caggttgtcg ctgcacacct ggttcggggc
     7321 gcagtcgctg tccgccatgc acgacagcac gcagtcgggg tcgagcggga acttggcgtc
     7381 gcaggcgttc gggcaggtcg ggtcgccggg cgggcacgcg cagtccgggt cgcccggcag
     7441 gttcgcgtcg caaaagccgt cgctgcacat cgggtcgtcc ggcgcgcagc agtcgacgtc
     7501 ctcgggcagc atcgggttac agacgccgtc gccgcaggtc gggtcgttcg ggtcggcgca
     7561 gacgcagccg ccgttgacgc acacctggcc cgcctggcag tcgctgtcgg cgacgcacag
     7621 gtcggcgcag tccgggtcgc cgggcagcat cggatcgcag accccgtcgc cgcagttcgg
     7681 gtcgttatcc gggcagttgc agtccgggtc gccgggcagc atcgggtcgc agaccccgtc
     7741 gccgcagctc gggtcgttcg ggtcctggca cacgcagtcc gggtcgccgg gggtcgccga
     7801 gcacacgccg tcgctgcagt tcgggtcgtc cggggcgcag cagtcggggt cgtccgcctg
     7861 catcggatcg cagacgttgt cggggcagcc ggggtcgtcg cccgggcact tgcagtccgg
     7921 gtcgccgacg ttcatcgggt cgcagacccc gtcgctgcag ttcggcgtgt tcgggtcgca
     7981 gccgcagttc gggtcgcccg gcgcacaaac cgggcagtcc gggtcggcca tgttcatcgg
     8041 gtcgcagatc ccgtcgccgc agtccggcgt gttcgggtcg cagccgccct cgtcggtcgc
     8101 gccgaggcag tccgggtcgt tcggaccgca ggtgtcaccg gcctcctgac cctgctcggc
     8161 cccgctgtcg tcacagtcgc cctcgtcgtc ctcgtcgatc tgcttgggca agggacagcc
     8221 ggcgttgccg agcgtgccga ccacgccgag ggtcgtgaag aggaagggag agagcagtcg
     8281 tagaattcgt gtcgtggtca tcgtcgctcc tgcaccggcg cgcgcgtcgg tcgtgaaagt
     8341 tcaaggtcgt cgtgctgtct ttccgcgagc ggcggatcga ttacaggctc gtccagcgcg
     8401 tgctcctccg cgccaccgct cgacgagcaa ccatccgtct cgccggcgat gacgaagtca
     8461 tcggtgtcgc agccgaacag cagcaccagc gcacacaaac atccgatcgt tctcatgggc
     8521 tctcctgctc gccggcctcg cggcgatgcg cgccggccgg gaaattttcc aaatatcggg
     8581 cccagcaggc gccgtgctcg tcatccgggg ccgtgcggca cagcccggcg tgggcgtcgt
     8641 cggcgaagcc gccgcggggg aaacgctcga ggtaggcgcg ccacagccgg cgctgcgagg
     8701 cggtgtcccg ccgcgtgcgc gcgagaccga accggtcggc gaaggcgtgc tcgacctccg
     8761 gccgcgtgcc accgatcgcc gcgatctcga cgtaacgctc gtcggcaccc gcgaggtcgc
     8821 ccgcgccgag cagcgcctcg gccgcgtcgt cgagccggcg cagacgcgtc gccaggtcct
     8881 cgcccgcgcg cggccgccga cgcacgaccg gcgcctccgg ccgcgacccg cagcccggcg
     8941 cgcagcccgg ctgtcccgcg ggacaggtgc actgcgcgac gcaggcctgg cccgcgggac
     9001 aggtctgacc ggccgccccg tccgtgcaga cctgtccttc gggacatgtc accgcaggcg
     9061 caggcggccg ctcgcgcgcc gcctcgacgg ccgcacggcg gtccgccacg gccccgagca
     9121 gccccgcgag cgagcccggg
//
LOCUS       DASWNJ010000822         1707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_367624, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000822 DASWNJ010000000
VERSION     DASWNJ010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367624"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1707
                     /locus_tag="VGB85_13970"
     CDS             <1..>1707
                     /locus_tag="VGB85_13970"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEY0135189.1"
                     /translation="VGMATVGLGLLGAAVVVLLYRSDAPNVLEGFGFGAALLAMFMRV
                     GGGIFTKAADVGADLVGKVEQGIPEDDPRNAATIADNVGDNVGDCAGMAADLFESYAV
                     TLVAALILGQVAFGSEGLVFPLIVPAIGVLTALVGIYGTSPRDGDRNGMNAINRGFFL
                     SAVVSAVLVAIAAFLYLPGDFAELSGGTESASDPRLVAIGAVVIGIVLASLIQVLTGY
                     FTETNRKPVQEIGKSSLTGAATVVLAGISVGLESAIYSSLLIGGAVYAAFLLGGGSTI
                     VALFAVALAGTGLLTTVGVIVAMDTFGPISDNAQGIAEMSGEVTEEGALVLTSLDAVG
                     NTTKAITKGIAIATAVLAATALFGSFRAEVGRALETAGRSADDFNLSIEQPNLLFGLI
                     IGAAVVFLFSGLAIQAVSRAAGRVVFEVREQFRTKPGIMDYTEKPDYGRVVDICTKDS
                     LRELATPGLLAVLSPIAVGFALGYQPLGAFLAGAIASGVLMAVFLSNAGGAWDNAKKL
                     VEDGNHGGKGSAAHEATIIGDTVGDPFKDTAGPALNPLLKVMNLVALLIAPSVVQYAI
                     GDD"
BASE COUNT          224 a          635 c          569 g          279 t
ORIGIN      
        1 gtcggcatgg ccacggtggg cctcggcctg ctcggtgccg ctgtcgtcgt cctgctgtac
       61 cgctcggacg cccccaacgt gctcgagggc ttcggcttcg gtgccgcgct gctcgccatg
      121 ttcatgcgcg tcggcggcgg catcttcacc aaggccgcgg acgtcggcgc cgacctggtc
      181 ggcaaggtcg agcagggcat ccccgaggac gacccgcgca acgcggcgac catcgccgac
      241 aacgtgggcg acaacgtcgg tgactgcgcc ggcatggcgg ccgacctgtt cgagtcgtac
      301 gccgtcacgc tcgtggctgc gctcatcctc ggtcaggtcg ccttcggctc cgagggcctg
      361 gtcttcccgc tcatcgtccc ggcgatcggc gtgctcaccg cgctcgtcgg catctacggc
      421 acgtcgccgc gcgacggcga ccgcaacggc atgaacgcca tcaaccgcgg cttcttcctc
      481 tcggccgtcg tgtcggccgt gctcgtcgcg atcgctgcgt tcctctacct cccgggcgac
      541 ttcgccgagc tgtccggcgg caccgagtcc gccagtgacc cgcgcctggt cgccatcggc
      601 gccgtcgtca tcggcatcgt gctggcgagc ctgatccagg tgctcaccgg ctacttcacc
      661 gagaccaacc gcaagccggt ccaggagatc ggcaagagct cgctcacggg ggcagcgacc
      721 gtggtgctcg cgggcatctc ggtcggcctg gagtccgcga tctacagctc gctgctcatc
      781 ggtggcgccg tctacgcggc gttcctgctg ggcggcggct cgaccatcgt cgcgctgttc
      841 gccgtggcgc tcgccggcac cggcctgctc accaccgtcg gcgtcatcgt cgcgatggac
      901 accttcggcc cgatcagcga caacgcgcag ggcatcgccg agatgtccgg cgaggtcacc
      961 gaggagggcg ccctcgtcct caccagcctc gacgccgtcg gcaacaccac caaggcgatc
     1021 accaagggca tcgcgatcgc gacggcggtg ctggccgcga ccgcgctgtt cgggtcgttc
     1081 cgcgcggagg tcggccgtgc gctcgagacg gcgggtcgca gcgccgacga cttcaacctg
     1141 tcgatcgagc agcccaacct gctgttcggc ctgatcatcg gtgctgcggt ggtgttcctg
     1201 ttctccggcc tcgcgatcca ggcggtgtcc cgcgcggcgg gccgcgtggt gttcgaggtg
     1261 cgcgagcagt tccgcaccaa gcccgggatc atggactaca ccgagaagcc ggactacggc
     1321 cgcgtcgtcg acatctgcac caaggactcg ctgcgcgagc tcgccactcc gggcctgctc
     1381 gccgtcctgt ccccgatcgc ggtcgggttc gcgctcggct accagccgct cggcgccttt
     1441 ctcgccggcg ccatcgcgtc cggcgtcctc atggcggtgt tcctgtccaa cgcgggcggc
     1501 gcgtgggaca acgccaagaa gctcgtcgag gacggcaacc acggcggcaa ggggtccgcg
     1561 gcccacgagg ccacgatcat cggcgacacg gtcggcgacc cgttcaagga caccgccggc
     1621 ccggcgctca acccgctgct caaggtcatg aacctggtcg cgctgctcat cgccccgagc
     1681 gtcgtgcagt acgccatcgg cgacgac
//
LOCUS       DASWNJ010000823         1624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_440782, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000823 DASWNJ010000000
VERSION     DASWNJ010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440782"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="VGB85_13975"
     CDS             <1..1094
                     /locus_tag="VGB85_13975"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEY0135190.1"
                     /translation="ASFIDVKKDVLRSERTEDHAQFGVEDPADVNAKGRGQRVTIKDQ
                     AGTVLVDIILGKKLDDKEGFRYVRIPDQKRVYASKIEVDISTNFTDWIEKDLLKIEQD
                     AIVAFLSDSYKVDETQGKVTNRDPLLIRRQPDPSKPDDKEGTWVIGAETPGIPGKELD
                     TGKIRQVLGGIDRLQIVGVRPRPEQLTLQALQSKGFFVTPDGRRLFGNEGEVKAFTND
                     GVVYTLYFGEITLDVGLALTAGTDDPAKPAEAKPEGEAEKEANRFMFVDVAYDPASDQ
                     TVNPAGPPPEEPEGAKRAKQLQARFDKWFYVISDASFKQIRKDKAELFKDAAAGSGST
                     PATPGTPAKKPATPPAAPAKKPATPAPAK"
     gene            1251..>1624
                     /locus_tag="VGB85_13980"
     CDS             1251..>1624
                     /locus_tag="VGB85_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135191.1"
                     /translation="MSTEDGEATRPRLSWREGLYLVGPHPLTTPYYASGFLLVAGVGY
                     ATPLVQIGLYLLLLLLAPLYIEAVLLTLSNGGTYMMTRYGLSHLGRLALIAAALVGVI
                     ISISYVATAIASLLAYGEYVSAL"
BASE COUNT          299 a          579 c          523 g          223 t
ORIGIN      
        1 cggcctcctt catcgacgtc aaaaaggacg tcctgcgcag cgagcgtacc gaggaccacg
       61 cgcagttcgg cgtcgaggat ccggccgacg tgaacgccaa gggcaggggc cagcgcgtca
      121 cgatcaagga tcaggccggc acggtgctgg tcgacatcat cctcggcaag aagctcgacg
      181 acaaggaggg ctttcgctac gtccgcatcc ccgatcaaaa gcgcgtgtac gcgtcgaaga
      241 tcgaggtcga catctcgacc aacttcaccg actggatcga aaaggacctg ttgaaaattg
      301 agcaggacgc gatcgtcgcg ttcctcagcg acagctacaa ggtcgacgag acccagggca
      361 aggtcaccaa ccgcgacccg ctgctgatcc gtcgccagcc cgatcccagc aagcctgacg
      421 acaaggaggg cacgtgggtg atcggcgccg agacgccggg gatcccaggc aaggagctcg
      481 acaccggcaa gatccgccag gtgctcggcg gcatcgaccg cctgcagatc gtcggcgtgc
      541 gcccgcggcc cgagcagctg acgctgcagg ccctgcagag caagggcttc ttcgtcaccc
      601 ccgacggccg gcgcctgttc ggcaacgagg gtgaggtcaa ggccttcacc aacgacggcg
      661 tcgtctacac cctctacttc ggcgagatca cgctcgacgt cggcctcgcg ctgaccgccg
      721 gcaccgacga cccggccaag cccgccgagg ccaagcccga gggcgaggcc gagaaggagg
      781 ccaaccgctt catgttcgtc gacgtcgcct acgacccggc cagcgaccag acggtcaacc
      841 ccgccggtcc gccgcccgag gagcccgagg gcgccaagcg ggccaagcag ctgcaggctc
      901 gcttcgacaa gtggttctat gtaatctcgg acgcgagctt caaacagatc cgcaaggaca
      961 aggcggagct gttcaaggac gccgcggccg gctccggcag cacgccggcc acgcccggca
     1021 caccggcgaa gaagccggcc acgccccccg cagcgcccgc caaaaagccg gcgactccgg
     1081 cccccgcgaa gtaactcctg cgaagtaggg ccgcgaagcc cgcggcaacg acggcgaggt
     1141 tcgatgcaag cgcatcgtcc ctcgccgttt gtgtttggtt cggtatcgcg ccgggtccgg
     1201 cgccggttgt cagcgctcgc ggggcggggc ataagtcgca agagcagcgc atgagcaccg
     1261 aggacggcga agcgacgcga ccccgcctca gctggcgcga gggcctgtac ctggtcgggc
     1321 cccacccgct gacgaccccg tactacgcct ccggcttctt gctggtcgcg ggcgtcggct
     1381 atgcgacgcc gctggtccag atcggcctgt acctgctgct gctgctgctc gccccgctgt
     1441 acatcgaggc cgtgctgctg acgctcagca acggcggcac ctacatgatg acgcgctacg
     1501 gcctgagcca cctcggccgc ctcgcgttga tcgccgcggc gctggtcggc gtcatcatct
     1561 cgatctcgta cgtcgccacc gcgatcgcca gcctgctcgc ctacggcgag tacgtcagcg
     1621 cgct
//
LOCUS       DASWNJ010000824         2587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_263243, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000824 DASWNJ010000000
VERSION     DASWNJ010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263243"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VGB85_13985"
     CDS             complement(<1..182)
                     /locus_tag="VGB85_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135192.1"
                     /translation="MPPVRLSALALCLSFAACQSVGPRSLQYGRGQYNVAIQRTNGEQ
                     LLLNLVRLRYRDAPLFL"
     gene            complement(278..1663)
                     /locus_tag="VGB85_13990"
     CDS             complement(278..1663)
                     /locus_tag="VGB85_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135193.1"
                     /translation="MRIAKASLSTCLCLPLLACGDAGSGSASASESGTTQGPTNPTTL
                     TPTTGETPTTSAPATTTGESASASGTESTANLSATDTGPDTGGGPKFDVGGQPDIAGG
                     GCGDGMGGMPEFSYIWVANSAEGTISKIDTQTLQEQGRYIVRPDSAGSPSRTSVNLSG
                     DVAVANRMGGVTKVYADVADCQDTNGTPGIQTSTSATSLPWGVEECIAWHTPFAFTTQ
                     RPVAWSPGTINPVTCEYESQKLWTAGGQNSVADSLVIHRLNGETGLIEDTIAMPDVFH
                     GYFGAYGGAVNQAGDFWFITYDIPSTLVRVDAKTLAYEKITVPDGLCPYGFAVDSKGR
                     PWTGSFCQASARYTPETATWDQFPVLGYGLQQDAEGRMWIADYSLPGTRAIDPETLQT
                     LTSISLPSGSVKGISIDFYGYVWVVDMQASAFRIDPDTQEFQSYDGLVGPYTYSDMTG
                     WGLKNVANPQG"
     gene            complement(1713..2294)
                     /locus_tag="VGB85_13995"
     CDS             complement(1713..2294)
                     /locus_tag="VGB85_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135194.1"
                     /translation="MAARGTNKKSGKSATKTKSAAKATKSAAEKATKKTAAKKKTKTA
                     AKTKTAAKKTTKAAAKAKATKVVARHTGTGRTAKTAANSTYAVARLRGLLDDSASVGE
                     WFERIPDWKVLGGYLDAALRAIADSDEILELLPVKARRLARRGPAIFAIFNAILDDEE
                     IDHRYLLDKLIRLVAPHLPLDRATLGRVAALIA"
     gene            complement(2367..>2587)
                     /locus_tag="VGB85_14000"
     CDS             complement(2367..>2587)
                     /locus_tag="VGB85_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135195.1"
                     /translation="GAAVTLPSEDRERLLDTLRRVDPGEFRAQVRAVLAAYGDVTGSA
                     RALRIAAPTLARWIASDPSLAAGLDLEP"
BASE COUNT          341 a          816 c          988 g          442 t
ORIGIN      
        1 aggaacagcg gcgcgtcgcg gtaacgcagg cgcaccaggt tgagcagcag ctgctcgccg
       61 ttggtccgct ggatcgcgac gttgtactga ccgcggccat actgcagcga gcgcggcccg
      121 accgactggc aggcggcgaa cgagaggcac agggcgaggg cagacaggcg cacgggcggc
      181 atcgcgccgg gatgctaacg cgaggctccg ggcttaccaa caggaggaac ccggagcgcc
      241 cgcggcgcat tttagtccgc ggtggcggcg agggcgatca gccctgcggg ttggcgacgt
      301 tcttgaggcc ccagccggtc atgtcgctgt acgtgtaagg cccgacgagg ccgtcgtacg
      361 actggaactc ctgggtgtcg gggtcgatcc ggaaggcgct cgcctgcatg tcgacgaccc
      421 agacatagcc ataaaagtcg atgctgatcc ccttcacgga gcccgagggc agcgagatcg
      481 aggtcagcgt ctgcagcgtc tccgggtcga tcgcgcgggt gcccgggagg ctgtagtcgg
      541 cgatccacat gcggccctca gcgtcctgtt gcaggccgta gccgagcacc gggaactggt
      601 cccaggtggc ggtctcgggg gtgtagcggg cgctggcctg gcagaacgat ccggtccagg
      661 ggcggccctt gctgtcgacc gcgaagccgt aggggcacag gccgtcgggg acggtgatct
      721 tctcgtacgc gagggtcttg gcgtcgacgc gcacgagggt gctcgggatg tcgtaggtga
      781 tgaaccagaa gtcgccggcc tggttgacgg cgccgccgta ggcgccgaag tagccgtgga
      841 agacgtccgg catcgcgatc gtgtcctcga tcaggccggt ctcgccgttg aggcgatgga
      901 tgacgaggct gtcggcgacg ctgttctggc cgccggcggt ccacagcttc tgcgactcgt
      961 actcgcaggt gaccgggttg atcgtgccgg gcgaccaggc gaccgggcgc tgggtggtga
     1021 aggcgaaggg ggtgtgccag gcgatgcact cctcgacgcc ccacggcagc gaggtcgcgc
     1081 tggtcgaggt ttggatcccc ggcgtgccgt tggtgtcctg gcagtcggcg acgtcggcgt
     1141 agaccttggt gacgccgccc atgcggttgg cgaccgcgac atcgcccgag aggttgacgc
     1201 tggtgcgcga ggggctgccg gcggagtcgg ggcgcacgat gtagcggccc tgctcctgca
     1261 gcgtctgcgt gtcgatcttg ctgatcgtgc cctccgcgct gttggcgacc cagatgtacg
     1321 agaactcggg catgccgccc ataccatcac cacagccgcc gccggcgatg tcgggctgcc
     1381 cgccgacgtc gaacttcggg ccgccgccgg tgtccgggcc ggtgtcggtc gcgctgaggt
     1441 tggcggtcga ctccgtgccg gacgcgctcg cgctctcacc cgtcgtggtc gcgggggcgg
     1501 aggtggtcgg tgtctcgccg gtggtcggcg tgagcgtggt cgggttggtc gggccctgcg
     1561 tggtgccgga ctcgctggcg ctcgcgctgc cgctgccggc gtcgccacag gcgagcaggg
     1621 gcaggcacag gcaggtcgac agcgaggctt tggcgatgcg catgcaacga agctacacgc
     1681 tttcgtgcgc gctcgcaaag cgccggcgcg ggtcaagcta tcagggccgc gacgcggccc
     1741 agggtcgccc tgtcgagcgg gaggtggggc gcgacgaggc ggatcagctt gtcgagcagg
     1801 tagcggtgat cgatctcctc atcgtccaag atcgcgttga agatggcgaa gatcgccgga
     1861 ccgcgccttg cgaggcggcg ggccttcacg ggcaggagct cgaggatctc gtcgctgtcg
     1921 gcgatcgccc gcagcgcggc gtcgagatag ccgccgagca ccttccagtc ggggatgcgt
     1981 tcgaaccatt cgccgacgct ggcgctgtcg tcgaggaggc cgcgcaggcg ggcgaccgcg
     2041 taggtcgagt tcgcggcggt cttcgccgtc ctgccggtcc cagtgtgcct ggcgaccacc
     2101 ttcgtcgcct tcgccttcgc ggcggccttc gttgtcttct tcgcggcggt cttcgtcttc
     2161 gcggcggtct tcgtcttctt cttggcggct gtctttttag tcgccttctc tgcggcggac
     2221 ttcgtcgcct tggcggcgga cttcgtcttc gttgcgctct ttccggactt tttgttcgtg
     2281 ccgcgggccg ccatgcattg gcggtatcag gcgtgggcgc gcggtgtcaa acccgaagcg
     2341 ggcgcgatgg ctcgcgcgcg accctttcac ggctcgaggt cgaggccggc ggcgagcgag
     2401 gggtcgctgg cgatccaccg cgcgagggtg ggggcggcga tgcggagcgc gcgggcgctg
     2461 ccggtcacgt caccgtatgc ggcgagcacg gcccggacct gcgcacgaaa ctccccggga
     2521 tcgacgcggc gcagcgtgtc gagcaggcgc tcacggtcct cggagggcag tgtcacggcc
     2581 gcgccag
//
LOCUS       DASWNJ010000825         1989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_298467, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000825 DASWNJ010000000
VERSION     DASWNJ010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298467"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..>1989)
                     /locus_tag="VGB85_14005"
     CDS             complement(112..>1989)
                     /locus_tag="VGB85_14005"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEY0135196.1"
                     /translation="RRRLGPAAVRRLPALWSWAATFALGLGFADLAGLPPWRGGLAIS
                     ASAAALPALLVLAAPGRARPALAFLLAAATAALLFADLLYMQTLGSIVPVQALLASHQ
                     VGDIGASITALAEPAYLWLAAPVLAGLLLALAWPRVPRDSLPRTASLGLAALAPVPFA
                     VAMVAAMAGPLGNRVFSEQHNVGRFGVIGAHAFDVLRTVRERLFRAGLSPERRAAVEQ
                     WFADRPPAASDPAFGVARGNNLLLIQAEAMQGWVLGAEVDGQKITPFLDSLRPRALTY
                     TTLADETAQGMTSDAEYAVLNSQLPLGQGAVAFLRADNRFVTLAHALRDAGHTTLSAH
                     PYKKGFWNRAVLHPRYGFDRSLFAEELGPGPTIGWGLSDDAFFKRIVPELIALPRPWF
                     AFLVTLSLHHPYDHFPDALKQLELGALEGTALGNYLHGMHYLDRSLAELFASLERAGL
                     ADDTVVAIYGDHDSRLPHTPELLRLAGVSAWTPAVPLRLDRVAGLVVLPGGQVRGEVD
                     AVGGHIDLAPTLLHYLGVPTPRCFAGAALLPGRSERGFAVFSDGSAIAGGRAFTAAGR
                     GVPLTGACFEHPSWRPLPLRECDALAERAQEALYVSRAVVDHDLAHDLAGVSPATPRG
                     P"
BASE COUNT          298 a          619 c          845 g          227 t
ORIGIN      
        1 gatctcgtcg tcgaggtcgt cctcctccga gaagtcgaag ttggcgcggc ggtcgtccgc
       61 ttgtgatggt cgggcggctt cagccatcgt cgcggcttcg aaggtagcag cttatggccc
      121 ccggggcgta gccgggctca cccccgcgag gtcatgcgcg aggtcgtgat ccacgacggc
      181 gcgcgagacg tacagcgcct cctgggcgcg ctcggccagc gcgtcgcatt cgcggagcgg
      241 cagaggtcgc caggaggggt gctcgaagca ggcgccggtc agcggcaccc cgcgaccggc
      301 ggccgtaaac gcgcgcccgc ccgcgatcgc ggaaccatcg gagaacaccg cgaaacctcg
      361 ttcggatcgc ccgggcagga gggccgcacc ggcaaagcag cgtggcgtgg ggaccccgag
      421 gtagtgcagg agcgtggggg cgaggtcgat gtggccgccg accgcgtcga cctcgcctcg
      481 cacctgtccc ccgggcaaga cgacgaggcc ggcgacgcgg tcgagtcgca gcgggacggc
      541 gggcgtccac gcggagacgc cggcgaggcg cagcagctcc ggcgtatgcg ggaggcggct
      601 gtcgtggtcg ccgtagatcg caacgacggt gtcgtcggcg aggccggctc gctcgagcga
      661 ggcgaacagc tccgcgagcg agcggtcgag gtaatgcatg ccgtgcaagt agttgccgag
      721 ggcggtgccc tcgagcgcgc cgagctcgag ctgcttgagc gcgtccggga agtggtcgta
      781 cgggtggtgc aggctgagcg tgacgaggaa ggcgaaccag ggccgcggca gggcgatcag
      841 ctcggggacg atgcgtttga aaaacgcgtc gtccgagaga ccccagccga tcgtcgggcc
      901 ggggccgagc tcctcggcga acagcgagcg gtcgaagccg tagcgcgggt gcaggacggc
      961 gcggttccag aagccctttt tgtaagggtg agccgagagg gtggtgtggc cggcgtcgcg
     1021 gagcgcgtgg gcgagggtga cgaagcggtt gtcggcgcgc aggaaggcga cggccccctg
     1081 accgagcggg agctgcgagt tgagcaccgc gtactcggcg tcggaggtca tgccctgggc
     1141 ggtctcgtcg gcgagggtcg tgtaggtgag ggcgcggggg cgcagggagt cgaggaaggg
     1201 ggtgatcttc tggccgtcga cctcggcgcc gagcacccag ccctgcatcg cctcggcctg
     1261 gatcagcagg aggttgttgc cgcgggcgac gccgaaggcc gggtcgcttg ccgcgggcgg
     1321 ccggtcggcg aaccattgct cgacggcggc gcggcgctcg gggctcaggc cggcgcggaa
     1381 cagccgctcg cgcacggtcc gcaggacgtc aaaggcgtgc gcaccgatca cgccgaagcg
     1441 gccgacgttg tgctgctcgg agaacacgcg gttgccgagc gggccggcca tcgccgcgac
     1501 catggcgacc gcgaacggca cgggggcgag cgccgcgagg ccgaggctgg ccgtgcgcgg
     1561 caggctgtcc cgcgggacgc gtggccaggc gagggcgagc aggagcccgg cgagcacggg
     1621 ggctgcgagc cacaggtagg cgggctcggc gagggcggtg atgctggcgc cgatgtcacc
     1681 gacctggtgg ctggcgagca gggcctgcac gggcacgatg ctgccgagag tttgcatgta
     1741 caggaggtcg gcgaacagca gggctgcggt ggcggcggcg agcaggaagg cgagcgccgg
     1801 ccgggcgcgg ccgggcgcgg cgagcacgag cagcgccggc aaggccgcgg ccgaggcgga
     1861 gatcgccagg ccgccgcgcc aggggggcag gccggcgagg tcggcgaagc cgaggccgag
     1921 ggcgaaggtg gcggcccagc tccacagggc gggcagccgg cgcacggcgg cggggccgag
     1981 gcgacgacg
//
LOCUS       DASWNJ010000826         1658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_474020, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000826 DASWNJ010000000
VERSION     DASWNJ010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474020"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="VGB85_14010"
     CDS             complement(<1..998)
                     /locus_tag="VGB85_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135197.1"
                     /translation="MKSHPLVLLALAFACQKPAAKPEEDVKAAAEVAGAAAAQVSAAG
                     GAVVATGKVTACPNSLGGTEQLHRVISKECGVVPVTEDYHVDGGSLTLEAGSSLSFKD
                     GAGLHIGHQSASSLIVQGTAKEPVVLTSAGDKSPGAWRGVVLHGNADRSQLEGLVIEY
                     VGDDEGALVIDAEDVIIKGVKLRETKATALRIGDVGSLKEFTGNEIRKVGNKTAIDTP
                     ARAIAGLGGGNRFDAGAQVLVRGGAVETSARWQDVGAPLLIAGEVALDGKDNQRITVE
                     LAPGLELRFSDLGLLTVGAQGPATLIAKGTSEAPITFTGQERRPGGWRNLGVHVYG"
     gene            1221..>1658
                     /locus_tag="VGB85_14015"
     CDS             1221..>1658
                     /locus_tag="VGB85_14015"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEY0135198.1"
                     /translation="MDPRDPSSSVSTGLLRDVQIPELLWQLHESGRTGCLKVEHGDVI
                     KQLWLRNGDPVFARSNQHSDRLTDRLLARGLITRSQYDAAQQLIAGNTGKRIGELMID
                     AGLIRPRELEESLSEHLLRMLDSMFLWQEGRWEFAAGVTSSEPV"
BASE COUNT          231 a          678 c          500 g          249 t
ORIGIN      
        1 tcgccgtaca cgtgcacgcc gaggttgcgc cacccccccg gccggcgctc ctgccccgtg
       61 aacgtgatcg gcgcctcgct cgtgcccttt gcgatcagcg tcgccgggcc ctgggctccg
      121 accgtcagca gcccgagatc cgagaagcgc agctcgagcc ccggcgccag ctcgaccgtg
      181 atccgctggt tgtccttgcc gtcgagcgcg acctcccctg cgatcaacag cggcgcgccg
      241 acgtcctgcc agcgcgccga cgtctcgacc gcgccgccgc gcaccagcac ctgcgcgccg
      301 gcgtcgaagc ggttgccgcc gccgagcccc gcgatcgcgc gcgccggcgt gtcgatcgcc
      361 gtcttgttgc cgaccttgcg gatctcgttg cccgtgaact ccttgaggct gccgacatcg
      421 ccgatccgca gcgccgtcgc cttcgtctcg cggagcttca cgcccttgat gatgacgtcc
      481 tcggcgtcga tcaccagcgc gccctcgtcg tcgccgacgt actcgatcac cagcccctcg
      541 agctgcgagc gatcggcgtt gccgtgcaac accaccccgc gccacgcccc cggagactta
      601 tcgcccgccg acgtcaacac caccggctcc ttcgccgtgc cctgcacgat caagctcgac
      661 gccgactgat gcccgatgtg cagccccgcc ccgtccttga agctcagcga cgaccccgcc
      721 tcgagcgtca ggctcccgcc gtcgacgtgg tagtcctccg tcaccggcac gaccccgcac
      781 tccttcgaga tcacgcggtg cagctgctcc gtaccgccga gagaattcgg gcacgccgtc
      841 accttgcccg tcgccaccac cgcgccgccc gccgcgctga cctgggccgc cgccgcgccc
      901 gcgacctcgg ccgccgcctt gacgtcctcc tcgggcttcg ccgcgggctt ttgacaggcg
      961 aacgcgagcg cgagcaggac cagcgggtgc gacttcatgg tgccgcggtt ttagtacgcc
     1021 gaacgaaacc cccgcaagtc ggccacgccc gttcccgccc gcagacccgg cgcctgtcct
     1081 tcgagacagc ccggcgcgcc cgcgcgccaa ctccgcatat ctccaggacg cctgcaaacc
     1141 atgcgcgttg gcccacgcgg ccgcccgcgg cacaccgccc ggagtcgact atcctgcggc
     1201 ttgcagcgcc cgttttggcc atggacccac gcgacccctc ctcctccgtc agcaccggtc
     1261 tgctgcgcga cgtgcagatc cccgagctgc tgtggcagct ccacgagtcg ggccgcaccg
     1321 gctgcctcaa ggtcgagcac ggcgatgtca tcaagcagct gtggctgagg aacggcgacc
     1381 cggtgttcgc gcgcagcaac cagcacagcg accgcctgac cgaccgcctg ctcgcccgag
     1441 gtctgatcac ccgcagccag tacgacgccg cccagcagtt gatcgccggc aacaccggca
     1501 agcggatcgg cgagctgatg atcgacgccg gcctcatccg cccgcgcgag ctcgaggagt
     1561 cgctgtccga gcacctgctg cgcatgctcg actcgatgtt cttgtggcaa gagggccgct
     1621 gggagttcgc ggccggcgtc acctcctccg agccggtg
//
LOCUS       DASWNJ010000827         2995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_50938, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000827 DASWNJ010000000
VERSION     DASWNJ010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50938"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VGB85_14020"
     CDS             <1..452
                     /locus_tag="VGB85_14020"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0135199.1"
                     /translation="DYDGLTYTRMVLDEVMRLRPPAWAMTRMAQRDDRLRGHLVEAGT
                     TVMFGIYFAHRHPDFWDEPEVFRPERFTPERIKARHSHAYIPFIAGPRTCIGRRFSIY
                     EMLVTLPAILRRFTVDVLPGQQVGMKVGATIHPDRPILARLLPVAGA"
     gene            538..2010
                     /locus_tag="VGB85_14025"
     CDS             538..2010
                     /locus_tag="VGB85_14025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0135200.1"
                     /translation="MADRSMFSDISHDVLIRVDAERRILAVNPECTRTLAAASEVLVG
                     SSLLDLLPPEQRDQVAARLAGEVSPDIDLRVSLRVGADTYRGFDLRWAGDAAGELTLA
                     ARAHATPDDTKRSELLLRSISECTQDYVVVTDADNHIIYANREPEEVARAGGMIGKPM
                     LAFAAPGQHEAIRGYVERVMTSGELVVYDSRAQFPDGSIHDFRSRLGPVRDLERSDRI
                     IGVVLITRDITAEVALEEAKRNAEQMLREYMVQLERSNSELERFASIASHDLQEPLRK
                     IQAFSDRLRDRFMAELPDPGRDYLERIRGAAKRMQDLINDLLMFSRLSAKEQTYARVP
                     LTKVARSVLSDLEVRVEETGGQVNLGELPMIDADPIHMRQLLQNLIGNALKFSRPGVA
                     PVVDITGDVVPGPDGPMLRLVVADNGIGIEPRHHDRIFGIFERLHGRGKYEGTGVGLA
                     VCRKIVEQHHGSITVTSVIDEGTKFTILLPMKQPERSQRT"
     gene            2007..2453
                     /locus_tag="VGB85_14030"
     CDS             2007..2453
                     /locus_tag="VGB85_14030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0135201.1"
                     /translation="MKHRRSITILIADDDPEDRMLAEDALKESRLVNDIRFVEDGEEL
                     LEYLQHRGKYADPESSPRPGMILLDLNMPRMDGREALKIIKTDPELRRIPVVVLTTSQ
                     AEQDIYRSYDLGVNSFIIKPVTFESLVNIMRVLEAYWFEIVEIPAS"
     gene            2629..>2995
                     /locus_tag="VGB85_14035"
     CDS             2629..>2995
                     /locus_tag="VGB85_14035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0135202.1"
                     /translation="MRRRISVLLVEDDEDDEVLARALLAEIDDLECSLTWARSFEDGL
                     RQLTEGSFDVCLLDYALGDRTGMELLTRAVASGIEVPVIFLTGQVERSLDIEAMRSGA
                     ADYLVKGRINADLLERSIRY"
BASE COUNT          502 a         1067 c          975 g          451 t
ORIGIN      
        1 aggattacga cggcctcacg tacacacgca tggtcctcga cgaggtcatg cggctacgcc
       61 cgccggcgtg ggcgatgacc cgcatggccc agcgcgacga tcgcctgcgc gggcacctcg
      121 tcgaggccgg cacgaccgtg atgttcggga tctatttcgc gcatcggcac cccgatttct
      181 gggacgagcc cgaggtgttc cgccccgagc gcttcacccc ggagcggatc aaagcccgcc
      241 acagccacgc ctatattccg ttcatcgccg ggcctcgcac ctgcatcggc cgccgcttct
      301 cgatctacga gatgctcgtg accctgccgg cgatcttgcg gcgcttcacg gtcgatgtgc
      361 tcccgggtca gcaggtgggc atgaaggtgg gagcgacgat ccaccccgac cggcccatcc
      421 tcgcgcgcct cctgccggtc gccggcgctt gacccgtcgc ccgcggcgcc ccgggcgatt
      481 cgatacaacg ttgcgaaatc ttgctatcgt gccccggcct gagaggaggc ctcgcgcttg
      541 gccgatagat cgatgttctc cgatatctcc cacgacgtgc tgatccgcgt cgacgcggag
      601 cgtcgcatcc tcgccgtcaa ccccgaatgc acgcgcacgc tcgcggccgc gagcgaggtg
      661 ctcgtcggct cctcgctgct cgacctgctg ccgcccgagc agcgcgacca ggtcgcggcg
      721 cggctcgccg gtgaagtgag cccggacatc gacctgcgcg tcagcctgcg cgtcggcgcg
      781 gacacctatc gcggcttcga cctgcgctgg gccggggacg ccgcgggcga gctgaccctc
      841 gcagcccggg cccacgcgac gccggacgac acgaagcgca gcgagctgct gctgcgatcg
      901 atctccgagt gcacccagga ctacgtggtc gtcaccgacg ccgacaacca catcatctac
      961 gccaatcgcg agccggagga ggtcgcgcgc gcgggcggca tgatcggcaa gccgatgctc
     1021 gcgttcgccg cgcccggcca gcacgaggcg atccgcggct acgtcgagcg ggtgatgacc
     1081 agcggcgagc tcgtcgtgta cgacagtcgg gcccaattcc cggacggcag catccacgac
     1141 tttcgcagcc gcctcggtcc cgtccgcgac ctcgagcgca gcgaccggat catcggcgtc
     1201 gtgctgatca cgcgggacat caccgccgag gtcgcgctcg aggaggccaa gcgcaacgcc
     1261 gagcagatgc tgcgcgagta catggtccag ctcgagcgca gcaacagcga gctcgagcgc
     1321 ttcgcgtcga tcgcctcgca cgacctgcag gagccgctgc gcaagatcca ggcgtttagc
     1381 gaccgcctgc gcgaccgctt catggccgag ctgccggacc ccgggcgcga ctacctcgag
     1441 cgcatccgcg gcgccgccaa gcgcatgcag gacctcatca acgacctgct gatgttctcg
     1501 cgcctgtccg ccaaggagca gacctacgcg cgcgtcccgc tgaccaaggt cgcccgcagc
     1561 gtgctctccg acctcgaggt gcgcgtcgag gagaccggcg gccaggtcaa cctcggcgag
     1621 ctgccgatga tcgacgccga cccgatccac atgcggcagc tgctgcagaa cctgatcggc
     1681 aacgcgctca agttctcgcg ccccggcgtc gcgccggtgg tcgacatcac cggcgatgtc
     1741 gtgccaggcc ccgacgggcc gatgctgcgc ctcgtcgtcg ccgacaacgg catcggcatc
     1801 gagccgcgcc atcacgaccg catcttcggc atcttcgagc gcctgcacgg gcgcggcaag
     1861 tacgagggca ccggcgtcgg cctcgcggtg tgccgcaaga tcgtcgagca gcaccacggc
     1921 agcatcacgg tgaccagcgt gatcgacgag ggcaccaaat tcacgatcct gctgccgatg
     1981 aagcagccgg agaggagcca gaggacttga agcaccgcag atcgatcacc atcctgatcg
     2041 ccgacgacga ccccgaggat cggatgctcg cggaggacgc gctcaaggag agccgcctcg
     2101 tcaacgacat ccgcttcgtc gaggatggcg aggagctgct cgagtacttg cagcaccgcg
     2161 gcaagtacgc cgaccccgag agctcccccc gcccgggcat gatcctgctc gacctcaaca
     2221 tgccgcgcat ggacggccgc gaggcgctca agatcatcaa gaccgacccc gagctgcggc
     2281 ggatccccgt cgtcgtgctc accacctcgc aggccgagca ggacatctac cgcagctacg
     2341 acctcggcgt gaattctttc atcatcaagc cggtcacctt cgagtcgctc gtcaacatca
     2401 tgcgcgtact cgaggcctac tggttcgaga tcgtcgagat cccggcctcg tagaacggtg
     2461 tatgcggggg gaaacggccg ggtcgagcgc gctggcccgg ccgccggaat ggctgcacga
     2521 ccgcgtagga atttgtagct tcaggtcgct cgaaggaggg tagcctctac tccgacacgc
     2581 tatcgcggcg gccgcagggg gcggcgcgtt caagggaggc cggccgacat gaggcgacga
     2641 atttccgtat tgctcgtcga ggacgacgaa gacgacgagg tgctggcgcg ggccctgctc
     2701 gcggagatcg acgacctcga gtgcagcttg acgtgggcgc gctcgttcga ggatggcctg
     2761 cggcagctca ccgaggggag cttcgacgtg tgcctgctcg actacgcgct cggcgaccgc
     2821 accggcatgg agctgctgac ccgcgcggtc gcctccggca tcgaggtgcc ggtcatcttc
     2881 ctcaccgggc aggtcgagcg ctcgctcgac atcgaggcca tgcgctcggg cgccgccgac
     2941 tatctcgtca agggtcgtat caacgcggac ctgctcgagc gatcgatccg ctatg
//
LOCUS       DASWNJ010000828         4354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_440828, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000828 DASWNJ010000000
VERSION     DASWNJ010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440828"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1788)
                     /locus_tag="VGB85_14040"
     CDS             complement(<1..1788)
                     /locus_tag="VGB85_14040"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY0135203.1"
                     /translation="MSGADERSPGPGARAWRAGLGLAIVVLLAVVCWLRMAVTTDITH
                     FLADETDAKLAGISRQLAQSELTRTIVLSVHAEGEDPGPALAAAASMAEALREHPEVA
                     WLRTGPGEVDGEAIHALYFPRRHFLLDDPDRLSDERLRAAAQALKQELGRPIGQMVKR
                     VAPADPLLLYPAQLRRLEAARAGGLTLVDGQFIAEGKYALVLLATRHSPFDAASQGPL
                     QAAIEGAFAEVQAGNPGLRLERSAVARFALSAEHSMRADIQRISTVSLVGLLLLFLIM
                     FRSPRLIVLAMVPLVAGVVAALAACLLVFGSVHGLTLAFGATLIGVCIDYSVHLFNHH
                     LLDPPRGGPEASARRIGPGLWLGAWTTIAGFAGLAWTSFPGLREIAVFASVGVFAAVV
                     ATRYWLPALMPRSSQVGRVPRVVADGLARGVAALVRRRAALFGLPAAAVLAMVIGVPR
                     IVWNDDIKLLTTTDPAMLAEDEGVRGRVARMDSGRFIVAIGSGVDVAAAEEAALVVND
                     AVHASLVAARAAGELAEFRSLHSFVRSAAAQQASLAALGDPTLPARLDAAFVAEGFRA
                     GSFAQFFADMSSLPGPVTPAGLASTPLADL"
     gene            complement(1801..2508)
                     /locus_tag="VGB85_14045"
     CDS             complement(1801..2508)
                     /locus_tag="VGB85_14045"
                     /inference="COORDINATES: protein motif:HMM:NF039988.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEY0135204.1"
                     /translation="MRPERPFAWLLLCACLIGGQAQAQAPVAAPVSTPVSTPAFASSP
                     ALQELLARFAAMSGLSAKFREEKRMALLAAPLVNEGVLYFAPRGRLARHITAPAPATV
                     LIDEGSLRYADAGGSETMSLDQNPVLRLFVESFVKIFAGDRTALERMYTLSLTELPAS
                     ADGVARWSLNLKPRVAPMTQIIDRIEITGHDVTLETMRVIEVGGDETVTTFSEVDTKR
                     RFTPQELKSLFTLPARR"
     gene            complement(2498..2923)
                     /locus_tag="VGB85_14050"
     CDS             complement(2498..2923)
                     /locus_tag="VGB85_14050"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY0135205.1"
                     /translation="MSETATSGRLHEVHHEFAVPFHDIDALRIVWHGHYYKYMELGRT
                     ALLRARGLDVRDIEGLGHRLLVVDSHCRHTFPLHYGDGVRVSAWFVSVAPRLVVAYRI
                     RNLTHDRCSARGQTILITTDADGHMLPETPHALLQRLAP"
     gene            complement(2920..>4354)
                     /locus_tag="VGB85_14055"
     CDS             complement(2920..>4354)
                     /locus_tag="VGB85_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HEY0135206.1"
                     /translation="YRARLAAGADHLARRLAAGEVVYGVTTGFGDSCVTSVPAEYVAQ
                     MPVNLMRFHGCGTGRIFDEVETAAIVVVRLVALAGCWSGVRPVVLERLCALLERRVLP
                     CIPAEGSVGASGDLTPLSYVVATLVGERECWVRGDRIPAAVALRDASIEPLTLAPKES
                     LALMNGTSVMVGLGVLAYVRAQRLARLCAALTAMTSDVLGGNPGHFDDRLFAAKPHPG
                     QRACARWIREDIAEDGPRLKPLPRIQDRYSVRCAPHIIGVLLDALPWIGTHLETEANG
                     ANDNPLIDPDTGEVLHGGNFYGGHACFALDALKTAVANLADLVDRQIAQICNPDMSHG
                     LPANLVGRTGPDRAAHHGFKAMQISSSALAAEALKLTMPASVFSRSTECHNQDKVSMG
                     TIAARDCLRILELTETAAVIGLLCLCQAVDLRAGVGCHARARALHAAVRAQVSMVDAD
                     RRMDHDIAHVLGMYRAGELPIGEVDFP"
BASE COUNT          679 a         1682 c         1453 g          540 t
ORIGIN      
        1 gaggtccgcc agcggcgtgc tcgccagccc ggccggcgtc accggacctg gcagcgagga
       61 catgtccgca aagaactgcg cgaacgagcc cgcgcgaaag ccctcggcga cgaaggccgc
      121 gtcgagccgg gccggcagcg tcggatctcc gagcgccgcc aggctcgcct gctgcgccgc
      181 ggcgctgcgc acgaagctgt gcaggctgcg aaactccgcc agctcgcccg ccgcgcgcgc
      241 ggccaccagg gacgcgtgga ccgcgtcgtt gacgacgagc gccgcctcct cggccgccgc
      301 gacgtcgacc ccgctgccga tcgcgacgat gaagcgcccg ctgtccatgc gcgcgacccg
      361 accgcgcacc ccctcgtcct cggcgagcat cgccgggtcc gtggtcgtca gcagcttgat
      421 gtcgtcgttc cagacgatcc gcgggacccc gatcaccatc gccagcaccg ccgccgccgg
      481 taagccgaac agcgccgcac gacgccgcac gagcgccgcc acgccccgcg ccagcccgtc
      541 cgcgaccacc cgcggcaccc tgccgacctg cgagctgcgg ggcatcagcg ccggcagcca
      601 gtagcgcgtc gcgaccaccg ccgcgaacac cccgacgctg gcgaacaccg cgatctcccg
      661 cagccccgga aagctggtcc acgccagccc cgcgaacccg gcgatcgtcg tccatgcccc
      721 cagccacagg ccgggcccga tccgccgcgc gctcgcctcg ggtccgcccc ggggcggatc
      781 gagcaggtga tgattgaaca ggtgcaccga ataatcgatg cacacgccga tcagcgtcgc
      841 cccgaacgcg agcgtcagcc cgtgcaccga gccgaacacc agcaggcacg cagccagcgc
      901 cgcgaccacc ccggcgacca gcggcaccat cgccagcacg atcaagcgcg gcgaccggaa
      961 catgatcaaa aacagcagca acagcccgac gagcgacacc gtcgagatcc gctgaatatc
     1021 ggcgcgcatg ctgtgctcgg ccgacagcgc gaagcgggcc accgcgctgc gctcgagcct
     1081 cagccccggg ttcccggcct gcacctcggc gaaggcgccc tcgatcgccg cctgcaacgg
     1141 cccctgcgac gccgcgtcga agggcgagtg gcgggtcgcc agcagcacca gcgcatactt
     1201 gccctcggcg atgaactgcc cgtcgaccag cgtcagcccg cccgcccgcg cggcctcgag
     1261 gcgccgcagc tgcgccggat acagcagcag cggatcggcc ggcgccaccc gcttgaccat
     1321 ttgcccgatc ggccggccga gctcctgctt gagcgcctgc gcggcggccc tgagccgctc
     1381 gtccgacaac ctgtccgggt catcgagcag gaagtgccgc cgcggaaagt acagcgcgtg
     1441 gatcgcctcg ccgtcgacct cacccggccc cgtgcgcagc cacgcgacct ccgggtgctc
     1501 gcgcaacgcc tcggccatgc tcgcggcggc cgccagcgcc ggcccgggat cctcgccctc
     1561 ggcgtgcacg ctgagcacga tcgtgcgcgt cagctcggac tgggccagct ggcgcgagat
     1621 gccggccagc ttcgcgtcgg tctcgtcagc caggaagtgc gtgatgtcgg tcgtgaccgc
     1681 catccgcagc cagcacacca ccgcgagcag cacgacgatc gccagcccga gcccggcgcg
     1741 ccacgccctc gcacctggcc ccggagaccg ctcgtccgcg cccgacacgc tcactgcatc
     1801 ctagcgcctg gccggcagcg tgaacagcga cttgagctcc tgcggcgtga agcgtcgctt
     1861 ggtgtcgacc tcggagaagg tcgtcaccgt ctcgtcgccg ccgacctcga tcacccgcat
     1921 ggtctcgagg gtgacatcgt ggcccgtgat ctcgatgcgg tcgatgatct gcgtcatcgg
     1981 agcgacccgc ggcttgagat tgagcgacca gcgcgcgacc ccgtcggcgc tcgccggcag
     2041 ctcggtcagc gacaacgtgt acatgcgctc gagcgcggtg cggtccccgg cgaagatctt
     2101 gacgaagctc tcgacgaaca gccgcagcac cgggttctgg tcgagcgaca tcgtctcgct
     2161 gccaccggcg tccgcgtagc gcagcgagcc ctcgtcgatc agcaccgtcg ccggcgcggg
     2221 cgcggtgatg tgccgcgcca gccgacccct cggcgcgaag tacagcacgc cctcgttgac
     2281 cagcggcgcg gccaacaacg ccatgcgctt ctcctcgcgg aacttcgccg acagcccgga
     2341 catcgccgcg aagcgagcca gcagctcctg caacgccggg ctgctcgcaa aagccggggt
     2401 ggaaaccggg gtggacaccg gcgcggccac cggcgcctgc gcctgcgcct gcccgccgat
     2461 caggcacgcg cacagcagca accacgcgaa cggacgctca gggcgcaagg cgttgaagca
     2521 gggcatgggg ggtctccggg agcatgtggc cgtcggcgtc ggtcgtgatg aggatcgtct
     2581 ggccgcgcgc cgagcagcgg tcatgggtga ggttgcggat ccggtacgcg acgaccagcc
     2641 gcggcgccac gctgacgaac catgcactga cgcgcacgcc gtccccgtaa tgcaacggga
     2701 aggtgtgccg gcagtgactg tcgaccacca gcaggcggtg accgagcccc tcgatgtcgc
     2761 ggacatcgag gccgcgcgcg cgcagcagcg ccgtgcgccc gagctccatg tacttgtaat
     2821 agtgcccgtg ccagacgatg cgcagcgcgt cgatgtcgtg gaagggcacc gcgaactcgt
     2881 ggtggacctc gtgcaggcgc ccggaggtgg ccgtctcgct catgggaagt cgacctctcc
     2941 gatcggcagc tcgccggccc ggtacatgcc cagcacatgg gcgatgtcgt gatccatccg
     3001 gcggtcggcg tcgaccatcg acacctgcgc gcgcactgcc gcgtgcagcg cccgcgcccg
     3061 cgcgtgacag ccgacccccg cgcgcaggtc gaccgcctgg cacaggcaca gcagcccgat
     3121 caccgccgcg gtctccgtca gctcgaggat ccgcaggcag tcgcgcgccg cgatcgtgcc
     3181 catgctcacc ttgtcctggt tgtggcattc ggtcgagcgc gagaagacgc tggccggcat
     3241 cgtcagcttc aacgcctcgg ccgccagcgc cgagctcgag atctgcatcg ccttgaagcc
     3301 gtggtgcgcc gcccggtccg gacctgtccg cccgaccagg ttcgccggca gcccgtggct
     3361 catgtcagga ttgcagatct gggcgatctg gcggtcgacg aggtcggcga ggttggcgac
     3421 cgccgtcttg agcgcgtcga gcgcgaagca ggcgtgcccg ccataaaaat tgccgccgtg
     3481 cagcacctcg ccggtgtccg ggtcgatcaa cgggttgtcg ttggcgccgt tggcctccgt
     3541 ctccagatgc gtcccgatcc acggcagcgc atcgagcaac acgccgatga tatgcggggc
     3601 gcagcgcacg gagtacctgt cctggatccg cggcaggggc ttcagacgcg ggccgtcctc
     3661 ggcgatgtcc tcgcggatcc accgcgcgca cgcgcgctgg cccgggtgcg gcttggcggc
     3721 gaacaggcgg tcgtcgaagt ggccggggtt gccgccgagc acgtccgagg tcatcgccgt
     3781 cagcgcggcg cacaggcgag cgagccgctg cgcccgcacg tacgcgagca ccccgagccc
     3841 gaccatcacg ctggtgccgt tcatcagcgc caggctctcc ttcggcgcca gcgtcagcgg
     3901 ctcgatgctc gcgtcgcgca gcgccaccgc ggccgggatc cggtcgccgc ggacccagca
     3961 ctcgcgctcg ccgaccagcg tcgcgaccac gtacgacagc ggcgtcaggt ccccgctcgc
     4021 cccgaccgag ccctccgccg ggatgcatgg cagcacccgc cgctcgagca gcgcacacag
     4081 gcgctcgagc accaccggcc gcacgcccga ccagcagccc gcgagcgcga ccagccgcac
     4141 gaccacgatc gccgcggtct cgacctcgtc gaagatgcgc ccggtcccgc agccgtgaaa
     4201 gcgcatcagg ttgaccggca tctgcgcgac gtactcggcc ggcaccgagg tcacgcacga
     4261 gtcgccgaag ccggtcgtga ccccgtacac cacctcgccc gccgcgagcc tgcgcgccag
     4321 gtgatcggcg cccgcggcca agcgcgcgcg atag
//
LOCUS       DASWNJ010000829         3741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_580656, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000829 DASWNJ010000000
VERSION     DASWNJ010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580656"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VGB85_14060"
     CDS             <1..631
                     /locus_tag="VGB85_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135207.1"
                     /translation="FLVPNEPSIVALETLGQSQRLTISAGTDAHQNVLPNKASDGERI
                     DSYRRMLRWFNNRVRLAGPLTPATAKAALRAGNNHIVFESFGTPIGFDFFSRMEQDEV
                     TTEMGAEVTLAAGMQLVAALPRLDPRSPQGDVPPAIEGRLYRATADNRELLRTWSEGA
                     LKLAAPGPGVYRVEVWISPRHLAPYLGDVAANYTETPVPWIYSGALFVR"
     gene            complement(628..1395)
                     /locus_tag="VGB85_14065"
     CDS             complement(628..1395)
                     /locus_tag="VGB85_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135208.1"
                     /translation="MNRNVTRIAVVHGPNPVHGATDPHLELPATLAAIEVGVMNVIGA
                     DMRPIVADADYVTDYSGPAPDGSGHRVYRGLGDVLDDEALAQTRVCVIRLLEAMLADS
                     ESWFLFVLGELDSGLPRPAARFVGRVDSIDLGGSFTIFEVAPGEIGDYAALFGARDRV
                     SGLAAPAGSLSSLLARLSLAAAVPTGQRYRFASPFHQHDSSALARVLEHGRLFFEETD
                     NGTRLRMIMPAHASAAVHERLTLAIEGSTAGEVDTDF"
     gene            complement(1668..2444)
                     /locus_tag="VGB85_14070"
     CDS             complement(1668..2444)
                     /locus_tag="VGB85_14070"
                     /inference="COORDINATES: protein motif:HMM:NF019240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1571 domain-containing protein"
                     /protein_id="HEY0135209.1"
                     /translation="MFSSPLRSARWAALVLVSAFAGPVAAQAPVAQTSDAAGADASQL
                     RQHVYAMEQAYSQVQDYTATFYKQERVKGRLLPVETMQLKFRKPFSVYLRWTGALEGR
                     EALYVRGWNDDKIRAHQGSFPDVTVNLRPDASLAMKGNRHPITQLGFGEVIKLMVRDA
                     RLSEVRPQDGVQYIDHGESTVYGARSHCLEAVTPARNYSPYYATRAKICFNVKTRMPT
                     RITVWNDEDVMIEDYGFEDVRLNVGLGDADFDPENPAYNF"
     gene            complement(2968..3480)
                     /locus_tag="VGB85_14075"
     CDS             complement(2968..3480)
                     /locus_tag="VGB85_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135210.1"
                     /translation="MHSFIHTLLSSILPALVVPALLTVATAAQASLLDISNCFYTQKV
                     QTTYGFDFSFGHSGKFTRNGISHPTTLEFDCSVYTTAHDVHCEGFMTLDDDIGAVLWT
                     FDGGTDKAAWKIDEVDIDIDFDHVYNEWGWNVMPDIIPVAGGLVRREDGSYYSSDWAA
                     IRDRITNLPI"
BASE COUNT          590 a         1182 c         1386 g          583 t
ORIGIN      
        1 gttcctggtc ccgaacgagc cgtcgatcgt cgcgctcgag accctcgggc agagtcagcg
       61 cctgacgatc tccgccggca ccgacgcgca tcaaaacgtg ctgcccaaca aggcgtcgga
      121 cggcgagcgc atcgactcgt atcgccgcat gctgcggtgg ttcaataatc gtgtgcggct
      181 cgccgggccg ctgaccccgg cgaccgccaa ggcggcgctg cgggccggca ataatcacat
      241 cgtgttcgag tccttcggca cgccgatcgg cttcgacttt ttttcaagaa tggaacagga
      301 cgaggtgacc accgagatgg gcgcggaggt gacgctcgcc gcgggtatgc agctggtcgc
      361 cgcgctgccg cggctcgacc cgcgcagccc gcagggcgat gtgccaccag cgatcgaggg
      421 ccggctttat cgcgcgacgg ccgacaatcg tgaattatta agaacgtgga gcgagggggc
      481 gctcaagctc gcggcgccgg ggcccggggt gtatcgcgtc gaggtgtgga tctcgccgcg
      541 tcacctggcg ccgtacctcg gcgacgtcgc cgcgaactac accgagacgc cggtgccgtg
      601 gatctacagc ggggcgctgt tcgtgcgcta gaagtcggtg tcgacctcgc cggcggtcga
      661 gccttcgatc gccagcgtca ggcgctcgtg taccgcggcg ctcgcgtggg ccggcatgat
      721 catgcgcagg cgggtgccgt tgtcggtctc ctcgaagaac agccggccgt gctcgagcac
      781 gcgtgccagc gccgagctgt cgtgctggtg gaacggcgag gcgaagcggt agcgctggcc
      841 ggtcggcacg gcggccgcca gcgacagccg cgcgagcagc gacgacaggc tgccggccgg
      901 cgccgcgagc ccggacacgc ggtcacgggc cccgaacagc gccgcgtagt cgccgatctc
      961 gccgggcgcg acctcgaaga tcgtgaacga cccgccgagg tcgatcgagt cgacgcgacc
     1021 gacgaagcgc gcggcgggcc gcggcaggcc gctgtcgagc tcgccgagga cgaacaggaa
     1081 ccatgactcg ctgtccgcga gcatggcctc gagcaggcgg atcacgcaga cgcgggtctg
     1141 cgcgagcgcc tcgtcgtcga gcacgtcgcc gagcccgcgg tagaccctgt ggcccgagcc
     1201 gtcgggcgcc ggcccggagt agtcggtcac gtagtcggcg tcggcgacga tcggccgcat
     1261 gtcggcgccg atcacgttca tcacgccgac ttcgatcgcc gcgagcgtcg ccggcagctc
     1321 gaggtgcggg tcggtggcgc cgtgtaccgg gttcggaccg tgcacgaccg cgatgcgcgt
     1381 cacgttacga ttcatcacgg ccgacatcag gtcacgggcc ggccgggcgc gtcaaggctt
     1441 cgttgctcgc tcaccgtccg cgcgcgactt catcgcgcgg cgagcccccg gagggcgtgc
     1501 gcatggacgg cgccagcggc ggcgaagtgc ggacggtcca cgcgcaggac ggtgatggag
     1561 gaggcgggcg ggcgcgtccc cgactggacg caggtcggcg tcgaggcaca aaaaagcgcg
     1621 accccggggg ggccgcgctc gctgtcccga aggacaggtg caaattttta gaagttgtag
     1681 gccgggttct cggggtcgaa gtcggcgtca ccgaggccga cgttgaggcg cacgtcctcg
     1741 aagccatagt cctcgatcat gacgtcctcg tcgttccaga cggtgatgcg ggtcggcatc
     1801 ctggtcttga cgttgaagca gatcttggcg cgggtggcgt agtagggcga gtagttgcgg
     1861 gccggggtga ccgcctcgag gcagtggctg cgggcgccat agaccgtgga ctcgccgtgg
     1921 tcgatgtact gcacgccgtc ctgcgggcgc acctcgctga gccgcgcgtc gcggaccatc
     1981 agcttgatga cctcgccgaa gccgagctgg gtgatcgggt ggcgattgcc cttcatcgcc
     2041 aggctggcgt cggggcgcag gttgacggtc acgtcgggga acgacccctg atgcgcgcgg
     2101 atcttgtcat cgttccagcc gcgcacgtag agcgcctcgc ggccctcgag cgcgccggtc
     2161 cagcgcaggt acacgctgaa gggcttgcgg aacttgagct gcatggtctc gacgggcagc
     2221 aggcgaccct tgacgcgctc ctgcttgtaa aaggtcgcgg tgtagtcctg cacctgcgag
     2281 tacgcctgct ccatggcgta gacgtgctgg cgaagctgcg aggcgtcagc gcccgcggca
     2341 tcggaggttt gcgcgacggg ggcctgcgcg gccaccggcc cggcgaacgc cgagacgagg
     2401 acgagcgcag cccaccgcgc ggagcggaga ggagacgaaa acatggcgcg gagcttgtgc
     2461 ataaaccctc cgggagtcaa acggggtgcg acagcgaggc gcgtgacgca cgtcgggggg
     2521 cgacgtcgac acgcggcgat ctcgagccct ctggatacga gggcgcgagg ggtcccatcg
     2581 acgggacaag cgaggccgta ttcacgaccc cgctgcgctg cgtctcgctt caacgcagca
     2641 ggatgaaaat agctccgatg cttgttctga tgcgggacag gcgtgcgcac agggaggttt
     2701 gtcagtgtcg gtttgtgcga gtggtcgctg tcgcgccact gtcgctatcg tgcctccggg
     2761 cctcctcgcg gttggcgctg gtcgggtcct ggcgcgcgat gaaaacctcc cggcccgcgc
     2821 gatgaaccgg gggtggcgtg ctgcgcacgg ccgcgggtct cgcgtcggtc gggtgcggag
     2881 agcgaccgac gcgtcgaggc cggaggcggt gtcccgcccc cggcgcccgg agaacccggc
     2941 gtgatccggg cggtgctaga tcgggggcta gatcgggaga ttggtgatcc ggtcgcggat
     3001 ggccgcccag tcggaggagt aataggagcc gtcctcgcgc ctgaccagtc cgccagcgac
     3061 ggggatgatg tccggcatga cgttccatcc ccactcgttg tagacgtggt cgaagtcgat
     3121 gtcgatgtcg acctcgtcga tcttccacgc ggccttgtcg gtgccgccgt cgaaggtcca
     3181 caggacggcc ccgatgtcat cgtcgagggt catgaagccc tcgcagtgga cgtcgtgggc
     3241 ggtcgtgtac accgaacagt cgaactcgag ggtggtcggg tggctgatgc cgttgcgcgt
     3301 gaacttcccg ctgtgcccga aggagaagtc gaagccgtac gtcgtctgga ccttttgcgt
     3361 gtaaaagcag ttcgagatgt cgagcaacga ggcctgcgcc gcggtggcga ccgtcagcag
     3421 cgccggcacg acgagcgcgg gcaggatgga ggacaggagg gtgtgaatga atgaatgcat
     3481 ggtgtctcgc tggttcgacg atgagtgagc gcggcgttcc ccgcagcacc gtgcgggcgt
     3541 cgcggcgtcg agcgccgtcg acgatccatt gccggcgagc tcgacgacgc tgacatcggc
     3601 gaccgaaaat atctttgtgc ccggccgggc gcggtcgctg cgtcaacagg tggacgcgcg
     3661 ccatcacgcg tcgcagcgag aacgcgacgg caggaagaga gtcagtcgat cgccagcctg
     3721 gctccgaccg tgcggccgta g
//
LOCUS       DASWNJ010000830         1869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_192537, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000830 DASWNJ010000000
VERSION     DASWNJ010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192537"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="VGB85_14080"
     CDS             <1..887
                     /locus_tag="VGB85_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135211.1"
                     /translation="GHQEAAVGALSRAISLAPQSLATLETLARALEQAGAWERAAAAW
                     ERHEKLVPARRRRDSRIGRGHAVAGQALAALTEGEGRKAERLAERALELAPDSGHVWT
                     TRARVSSSLGDAAVANVAWQRAWELTPAAATQLIAEALEAAAEIGALEDLHERMLASL
                     RTSDEAALVVALGRRLGSAHPEAVCAALERVRGRSPAAALTLVRLRLTQGQREQALDA
                     AMMEPSPPIVVCRACAREMPRFRFRCDDCGAWDSAVTLEGHAHRQVGDRERVAAHGVH
                     EAREAAREVDGRVRRAEA"
     gene            884..>1869
                     /locus_tag="VGB85_14085"
     CDS             884..>1869
                     /locus_tag="VGB85_14085"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY0135212.1"
                     /translation="MNREQSAAYPRRRSELLSTTSRFMRQWGYALFILMIIVVFRDVL
                     IPFIFAFALAYLMAPVIHRLQPRLGRALSVILVYLTLFGVIAVFMVVFLPALVQDFAR
                     LRDTAPEALEYLDKTYLPQATQWFEESFSSLNAAAPEIAPPPPSELFATPGPGGTWSI
                     SLERVQLSMQDAGNGQWLIHTPSQHQEQNLTDTLRKLVMNKGSEYTGQIAGFLRALLS
                     GVTTFLTNLVITLMLAAFILINPGRVMNFARGLVPLHYRETYEELMLLLDVGLAGVIR
                     GQLLICLVNGILTYIGLVIFGIKYSFLLAVVAAAFSLVPIFGTIISSIPIIIV"
BASE COUNT          254 a          697 c          639 g          279 t
ORIGIN      
        1 agggtcacca ggaggcggcc gtcggcgcgc tgtcgcgggc gatcagcctc gccccgcagt
       61 cgctcgccac gctcgagacc ctcgcccgcg cgctcgagca ggccggcgcg tgggagaggg
      121 ccgccgccgc ctgggagcgc cacgagaagc tggtccccgc ccgccgccgc cgcgacagcc
      181 ggatcggtcg gggtcacgcg gtcgccggtc aggcactcgc ggcgctcacc gagggggagg
      241 gccgcaaggc cgagcgcctc gccgagcggg cgctcgagct cgccccggac tccgggcacg
      301 tgtggacgac gcgggcccgc gtcagctcga gcctcggcga cgccgcggtc gccaacgtgg
      361 cctggcagcg cgcctgggag ctcacgccgg cggccgccac gcagctgatc gcagaggccc
      421 tcgaggcggc ggccgagatc ggcgcgctgg aggatctgca cgagcggatg ctcgccagcc
      481 tgcgcaccag cgacgaggcc gcgctggtcg tcgcgctcgg ccggcgcctc gggtccgcgc
      541 accccgaggc cgtgtgcgcc gcgctcgagc gcgtgcgcgg ccggtcgccc gccgcggccc
      601 tgaccctggt acgcttgcgg ctcacccagg gccagcgcga gcaggccctc gacgccgcga
      661 tgatggaacc ctcgccaccg atcgtggtct gccgggcgtg cgcccgcgag atgccgcgct
      721 ttcgcttccg ctgcgacgac tgcggcgcgt gggacagcgc cgtcacgctc gagggccacg
      781 cgcaccgcca ggtcggcgac cgcgagcgag tcgcggcgca tggcgtgcac gaggcacgcg
      841 aggcggcgcg tgaagtcgac gggcgcgtac ggagggcaga ggcatgaacc gcgaacagtc
      901 ggccgcatat ccgcggcgcc gcagcgagct gctgagcacc accagccgct tcatgcgcca
      961 gtggggctac gcgctgttca tcctgatgat catcgtcgtc tttcgcgacg tcctgatccc
     1021 gttcatcttc gcgttcgcgc tcgcctacct gatggcgccg gtgatccacc gcctgcagcc
     1081 gcgcctcggc cgggccctgt cggtcatcct ggtctacctg accctgttcg gcgtgatcgc
     1141 ggtgttcatg gtcgtgttcc tgccggccct cgtgcaggac ttcgcccgcc tgcgcgacac
     1201 ggctccggag gcgctcgagt acctcgacaa gacctacctg ccgcaggcga cgcaatggtt
     1261 cgaggagagc tttagcagcc tcaacgcggc cgcccccgag atcgccccgc cgcccccctc
     1321 cgagctgttc gccaccccgg gccccggcgg cacctggtcg atcagcctcg agcgcgtcca
     1381 gctgtcgatg caggacgcgg gcaatggcca gtggctgatc cacacgccct cgcagcatca
     1441 ggagcagaac ctcaccgaca cgctgcgcaa gctggtgatg aacaagggca gcgagtacac
     1501 cggccagatc gccggctttt tgcgggcgct gctcagcggg gtcacgacct tcctgacgaa
     1561 cctcgtcatc acgctgatgc tcgcggcgtt catcctgatc aacccgggac gggtgatgaa
     1621 cttcgcccgc ggcctggtcc cgctgcacta ccgggagacc tacgaggagc tgatgctgct
     1681 gctcgacgtc ggcctcgccg gcgtcatccg cggccagctg ctgatctgcc tcgtcaacgg
     1741 catcctgacc tacatcggcc tggtcatctt cggcatcaag tacagcttcc tgctcgcggt
     1801 cgtcgcggcg gcgtttagtc tggtgccgat cttcggcacg atcatctcgt cgatcccgat
     1861 catcatcgt
//
LOCUS       DASWNJ010000831         5392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_192570, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000831 DASWNJ010000000
VERSION     DASWNJ010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192570"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="VGB85_14090"
     CDS             complement(<1..964)
                     /locus_tag="VGB85_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135213.1"
                     /translation="MRTRPLLVFLAAALGGCNNDPSTTSEGTSTSGASEPTTDTSTTD
                     TPTSDPSTSDTPTTQSSSSGDTTGDPGDTSEDTDTTGGAPLTRVEQILHALDVAMYQC
                     PERIWPDVEASYRSRQVLLASEIENKAWLWNAQQGGGVPPAVLEGPLDSLPPEWTSIF
                     NINQLAGALTLGISLDWTAEQNADIVANGGIVWPDYATSLTFHEGFHFLSDQNDWNTG
                     NGSRSSPYPEPWEPRYLRAQLERALHEEIDSPGAGLGHAAHWQARLLAEHTEEMKAIR
                     SYDCTEGSAEYVSLMMSALAELGCDAGDDELLALAISHLPDGIFL"
     gene            1139..1381
                     /locus_tag="VGB85_14095"
     CDS             1139..1381
                     /locus_tag="VGB85_14095"
                     /inference="COORDINATES: protein motif:HMM:NF022716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3072 domain-containing protein"
                     /protein_id="HEY0135214.1"
                     /translation="MTTPNPKTEDLEELPSNREKDPDDWVTGDERMTGAQASYLKTLC
                     EEAKQEFDPEMTKAEASKRIDELQKVTGRGVLASES"
     gene            1708..3891
                     /locus_tag="VGB85_14100"
     CDS             1708..3891
                     /locus_tag="VGB85_14100"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEY0135215.1"
                     /translation="MQDKNKDLEADRKDSTDGRLTTNQGIPISDNQNSLKAGPRGPTL
                     LEDFVLREKITHFDHERIPERVVHARGSAAHGYFQVYQPLPHLTKAKLLNDPSLKTPV
                     FVRFSTVVGSRGSGDTARDARGFATKFYTEDGIWDLVANNIPVFFIQDAMKFPDLVHA
                     VKPEPHHDMPQAGSAHDNFWDFAWLTPEATHMVMWVMSDRALPRSYRMMEGFGVNTFR
                     LINEAGKAVLVKFHWKPLLGVHGLVWDEAQKIMGKDADFHRRDLWENIEQGNFPEYEF
                     GIQVIEEADERRFDFDPLDATKFWPESLVPVQRVGKLVLNRNPDNFFAETEQVAFCPA
                     NVVPGIDFSDDPLLQGRLFSYLDTQLSRLGGPNFHEIPINRPTCPVANTQRDGHKRHT
                     VNVGPVNYQPNSRGNGCPMQNGGSGFVTYPSRTDGGKVRERSESFNDHFSQATLFWNS
                     MSDPEKEHIVDAFRFELGKVETKHIRERMIDILLHVDQSLAHRVAYGIGVPVDGPGND
                     LASARKRLQEGWAKFGTTGLPGAKIGASTPAMAPELSMVVNPNYPKDSIATRKIAFLI
                     ADGVNCDQVDKVKAALMKAGATVELISTKLGPVTSAAGKTLEPDKSFLTAASVFYDAV
                     FVPGGSQSVGTLRGEGDAVHFINEAFKHCKALAAAGEGVDLLVSSNIVDQSGGAQQTE
                     TTIVALPGVVADRAATDARALATRFIAEIAQHRHFTRTQKERVPA"
     gene            complement(4005..5051)
                     /locus_tag="VGB85_14105"
     CDS             complement(4005..5051)
                     /locus_tag="VGB85_14105"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0135216.1"
                     /translation="MTRFSTVRNEAGDALPVAHGEGGLDGLLGFVDEHRSWLQDQLHT
                     RGAALLRGFGLATPAQFEAAARAVEPELKNEYLGTSPRDARTPYVFSASELPPYYPIP
                     QHCEMTFVKQPPRRLMFACLVAPRGGGGETPLCDMRTVAAQIDPAVRERFERGGIRII
                     RNYGGPSGKGRFDLWQLKRWDELFQTQDRAAVEARCAAEGVYTDLRWGPGDRLRLISE
                     HDALRVHPETGVPVWFNHVQVFHLSTAASELRRIYRRTSDLRSGLLWRFAQVAVAGKR
                     RFTAVDEQALHCTYRDGREIEVADLEHVRDTIWRHMIAEPWQQGDMVVIDNFAIAHGR
                     LPYRGPRQVVVAWA"
     gene            complement(5085..>5392)
                     /locus_tag="VGB85_14110"
     CDS             complement(5085..>5392)
                     /locus_tag="VGB85_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135217.1"
                     /translation="PQEGAALWVDYLAIPAAAEQVALALEFIDFVLDPEIAALNANFL
                     HYATPNRVALERGLITDKDDPQVYPPADVMSRLHVSENWFGDTENLVDKLWLELRGS"
BASE COUNT          867 a         1954 c         1757 g          814 t
ORIGIN      
        1 cgaggaagat cccgtccggc aggtgcgaga tcgccagggc gagcagctcg tcgtcgccgg
       61 cgtcgcagcc gagctccgcg agcgcgctca tcatcaggct cacgtactcg gccgacccct
      121 cggtgcagtc atagctgcgg atcgccttca tctcctcggt gtgctcggcg agcaggcgag
      181 cctgccagtg cgccgcgtgg cccaggccgg cgccggggct gtcgatctcc tcgtgcagcg
      241 cccgctcgag ctgcgcccgc aggtagcgcg gctcccacgg ctcggggtac ggcgacgagc
      301 ggctgccgtt gccggtgttc cagtcgtttt gatcgctgag gaagtgaaag ccctcgtgga
      361 aggtcagcga ggtcgcgtaa tccggccaca cgatgccgcc gttcgccacg atatcggcgt
      421 tctgctcggc ggtccagtcg agcgagatcc cgagggtcag cgccccggcg agctggttga
      481 tgttgaagat cgacgtccac tccggcggca ggctgtcgag cggcccctcg agcaccgccg
      541 gcggcacgcc accgccctgc tgcgcgttcc agagccacgc cttgttctcg atctccgagg
      601 ccagcaacac ctgccggctg cgatagctcg cctcgacatc gggccagatg cgctcggggc
      661 actggtacat cgcgacgtcg agcgcgtgaa ggatctgctc gacgcgggtc agcggcgcgc
      721 cgccggtcgt gtcggtgtcc tcgctcgtgt cgccggggtc gccggtcgta tcgccgctgc
      781 tcgaagattg cgtcgtcggc gtgtcgctcg tcgaggggtc gctcgtcggc gtatccgtcg
      841 tcgaggtgtc ggtcgtcggc tcgctcgcgc ccgaggtcga ggtcccctcg ctggtcgtgg
      901 acgggtcatt gttacagccg ccgagcgcgg ctgcgaggaa tacgaggagc gggcgggttc
      961 gcatatgcgg ctttagggtc acatcgcgcc cggcgcctgt ccactggaca ttgtgctccg
     1021 cggcgccccg tccgcagctt gctgctgcaa cgaagcacgc cggccgatct cgccacccag
     1081 ggcgcccgtg cctcgctctg cgcgtgcctg ctgcgcgcgc gcgcgtacgc tcccgggtat
     1141 gacgaccccc aacccgaaga ccgaggacct cgaggagctg ccgtccaacc gcgagaagga
     1201 ccccgacgac tgggttaccg gcgacgagcg catgaccggc gcgcaggcgt cgtatctcaa
     1261 gaccctgtgc gaggaggcca agcaggagtt cgaccccgag atgaccaagg ccgaggcctc
     1321 caagcgcatc gacgagctgc aaaaggtcac cggtcgcggc gtcctggcga gcgagtcgtg
     1381 agtcgcgtcg ctcgggcccg gcgccgccgg tgctcgcggc ggcgcccggt gcggcctcgc
     1441 tcccacgcgc agtgatggac gacctcgtgc gaccacacgt cacgcggcgc tgacctgacc
     1501 tttgggacat ccggccacga gcggccggcg attcgcgcca cacgcgcgcc agccggggtg
     1561 aacgggcccc gcgacggcca tatggcgcgc cgggtgatgc caaacgctgc cccgcccaaa
     1621 tcgcccaccg ccgctgtgcg tcgcgcttgc cgacccgggg cgcggtgcta tcgacccccg
     1681 ggcttttctc ggcccggagg atcgaagatg caagacaaga acaaggacct ggaagccgac
     1741 cgcaaggaca gcacggacgg ccgcctgacg accaatcagg gcattccgat ctcggacaat
     1801 cagaactcgc tcaaggccgg cccacgcggt ccgaccctgc tcgaggactt cgtcctccgc
     1861 gagaagatca cccacttcga tcacgagcgc atcccggagc gcgtcgtgca tgcccgcggc
     1921 tccgcggcgc acggctactt ccaagtctac cagccgctcc cgcacctgac caaggccaag
     1981 ctgctcaacg acccctcgct caagacgccg gtgttcgtgc gcttctcgac ggtcgtcggc
     2041 tcgcgcggct cgggcgacac ggcccgcgac gcgcgtggct tcgcgaccaa gttctacacc
     2101 gaggacggca tctgggacct cgtcgccaac aacatcccgg tcttcttcat tcaggacgcg
     2161 atgaagttcc cggacctcgt gcacgcggtc aagcccgagc cgcaccacga catgccgcag
     2221 gccggctcgg cccacgacaa cttctgggac ttcgcgtggc tgacccccga agcgacccac
     2281 atggtgatgt gggtcatgtc ggaccgcgcc ttgccgcgca gctatcgcat gatggagggc
     2341 ttcggcgtca acacctttcg cctgatcaac gaggccggca aggccgtgct cgtcaagttc
     2401 cactggaagc cgctgctcgg cgtgcacggc ctggtgtggg acgaggcgca gaagatcatg
     2461 ggcaaggacg ccgacttcca ccgccgcgac ctgtgggaga acatcgagca gggcaacttc
     2521 cccgagtatg aattcggcat ccaggtgatc gaggaggccg acgagcgccg cttcgacttc
     2581 gacccgctcg acgcgaccaa gttctggccg gagagcctcg tgccggtgca gcgcgtcggc
     2641 aagctggtgc tcaaccgcaa ccccgacaac ttcttcgccg agaccgagca ggtcgcgttc
     2701 tgcccggcca acgtcgtgcc gggcatcgac ttctcggacg acccgctgct gcaaggccgc
     2761 ctgttctcgt acctcgacac ccagctgtcg cgcctcggcg gccccaactt ccacgagatc
     2821 ccgatcaatc gtccgacctg cccggtggcc aacacccagc gcgacggcca caagcgccac
     2881 accgtcaacg tcgggccggt caactaccag cccaactcgc gcggcaacgg ctgcccgatg
     2941 caaaacggcg gctccggctt cgtcacctat ccctcgcgca ccgacggggg caaggtccgc
     3001 gagcgcagcg agagcttcaa cgaccacttt agccaggcga cgctgttctg gaacagcatg
     3061 tccgacccgg agaaggagca catcgtcgac gccttccgct tcgagctcgg caaggtcgag
     3121 accaagcaca tccgcgagcg catgatcgac atcctgctgc acgtcgacca gagcctcgcg
     3181 caccgcgtcg cctacggcat cggcgtgccg gtcgacggcc ccggcaacga cctcgcaagc
     3241 gcccgcaagc gcctgcaaga gggctgggcc aagttcggca ccaccggcct gcccggcgcc
     3301 aagatcggcg ccagcacgcc cgccatggcc cccgagctga gcatggtcgt caaccccaac
     3361 taccccaagg acagcatcgc gacccgcaag atcgcgttcc tgatcgccga cggggtcaac
     3421 tgcgaccagg tcgacaaggt caaggccgcg ctgatgaagg ccggcgcgac cgtcgagctg
     3481 atctcgacca agctcggccc ggtcaccagc gccgccggca agaccctcga gcccgacaag
     3541 agcttcctga ccgccgcgtc ggtcttctac gacgcggtct tcgtgccggg cggctcgcag
     3601 agcgtcggca ccctgcgcgg tgagggcgac gccgtgcatt ttatcaatga ggcgttcaag
     3661 cactgcaagg cgctcgccgc cgccggtgag ggcgtcgacc tgctggtcag cagcaacatc
     3721 gtcgaccagt ccggcggcgc ccagcagacc gagaccacga tcgttgcgct gccgggcgtc
     3781 gtcgccgacc gcgccgcgac cgacgccagg gccctcgcca cccgcttcat cgccgagatc
     3841 gcccagcacc gccacttcac gcgcacccag aaggagcgcg tgccggcctg atgcgctgag
     3901 ccgtttcacg gccctccggg cctgagccgc ttcacggccc tccgggcctg agcaagtgcc
     3961 ccgaacacgc ctcgcgtgct cggggctctt gcttttttaa agtctcaggc ccaggcgacg
     4021 acgacctgcc gcggtccacg atacggcagc cgcccgtgcg cgatggcaaa attatcgatg
     4081 accaccatgt cgccctgttg ccacggctcg gcgatcatgt ggcgccagat cgtatctcgc
     4141 acgtgctcga ggtccgcgac ctcgatctcg cgtccgtcgc ggtacgtgca atgcagcgcc
     4201 tgctcgtcga ccgccgtgaa cctgcgcttg ccggccacgg cgacctgcgc gaagcgccac
     4261 agtaacccac tgcgcagatc cgacgtccgg cgatagatcc gtcgcagctc ggaggccgcc
     4321 gtcgacaggt ggaacacctg gacgtggttg aaccatacgg gcacgcccgt ctcggggtgc
     4381 acccgcaggg catcgtgttc gctgatcagt cgcaggcggt cgccgggtcc ccagcgcagg
     4441 tcggtatata cgccctcggc cgcgcagcgg gcctcgacgg ccgcgcggtc ctgcgtctgg
     4501 aacagctcgt cccagcgctt gagctgccac aggtcgaagc ggcccttgcc cgagggtccg
     4561 ccatagttgc gaatgatgcg gatcccgccg cgctcgaagc gttcgcgcac cgccggatcg
     4621 atctgcgccg ccaccgtccg catgtcacaa agcggcgtct cgccgccgcc gccgcgcggc
     4681 gcgaccaggc acgcgaacat cagccggcgc ggcggctgct tcacgaaggt catctcgcag
     4741 tgctgcggga tcgggtagta gggcggcagc tcgctcgcgg agaacacgta gggcgtccgc
     4801 gcgtcgcggg gcgacgtgcc gagatattcg ttcttgagct ccggctcgac cgcccgcgcc
     4861 gcggcctcga attgcgccgg cgtggcgagc ccgaagccgc gcagcagcgc cgcgccgcgg
     4921 gtgtgcagtt gatcttgcaa ccaactccgg tgctcgtcga caaagccgag caggccgtcg
     4981 aggccgccct cgccgtgcgc caccgggagc gcgtcgcctg cctcgttacg caccgtggag
     5041 aaccgggtca cgccggtatt atggcaccac tgcgcgcccc ggtcctagct cccgcgcagc
     5101 tcgagccaga gcttgtcgac caggttctcg gtgtcgccga accagttctc cgagacatgc
     5161 aggcgcgaca tcacgtccgc gggcggatag acctgcgggt cgtccttgtc ggtgatgagg
     5221 ccgcgctcga gcgcgacccg attgggtgtc gcgtagtgca ggaagttggc gttgagggcc
     5281 gcgatctccg gatctagcac gaagtcgatg aactcgagcg ccagcgcgac ctgctcggcc
     5341 gccgcgggga tcgccaggta gtcgacccac agcgccgcgc cctcctgcgg ga
//
LOCUS       DASWNJ010000832         3110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_298572, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000832 DASWNJ010000000
VERSION     DASWNJ010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298572"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VGB85_14115"
     CDS             complement(<1..222)
                     /locus_tag="VGB85_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135218.1"
                     /translation="MIAASGGSSSAALLRASALAMLVSAAACPSPGASDRQNTSIPVS
                     AVDVPQAPTVPTTGAGRPLAQLCPSALPNR"
     gene            370..975
                     /locus_tag="VGB85_14120"
     CDS             370..975
                     /locus_tag="VGB85_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135219.1"
                     /translation="MPSGPELCDGLDNDCDEQVDDECICSADADCGGADSGKVCDASD
                     KCTDGCHANGNGCPEGEVCTSESDEIGMCVPAGGTSDTGATEGDPSSGTGPVTGSGNS
                     DSATGDPDGLSTGDVPTSGATDVGASETTDADPGTGETGGQDSGEGGCGCDVRDPART
                     GCCSACSVCSDLAAAPSRVRPRPGPGPRRIVTSARRADAGS"
     gene            1426..1599
                     /locus_tag="VGB85_14125"
     CDS             1426..1599
                     /locus_tag="VGB85_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135220.1"
                     /translation="MRPVDQSAGLETSSDPDPRHTAPGFVYDLRVCLKKYARAGCIFN
                     YALRERPQIAGGR"
     gene            1702..3060
                     /locus_tag="VGB85_14130"
     CDS             1702..3060
                     /locus_tag="VGB85_14130"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEY0135221.1"
                     /translation="MNFEAIQETFLSLFVNSSDLRLYVLLKYPELDHDLPLEGPSTRQ
                     YSVAVLQLLSRRGYLDVAFFAGLISRFPRRAIDIRSAAKLLLGGEVEVLLGENLPEAR
                     AASLRPDQDPKTDSPLSQWKTLCQCIHQGRVLPIIGPDLDEGLFGGTGALATRLATKY
                     ALIKSDHERADLAKVAQFLSVERGRDFAHKAVQAELINALIEHQGASIGEQVHELPKL
                     LDSIVEYRRARLDDPYAAVAKLPASIFITASPETLLFKTVKSVGKNPEALVCRWRSSG
                     NNIPREPRPKQPPGPETPLVHHIFGVFGVPESLVFTEDDLYDFLIATSTYKLIPASVR
                     GSLTDSALLFLGFRLDDWTFRVLFRMIMNLEGSAAMRKYSHVGVQVTPGENSRADVER
                     ARRHLERYFGSDRGAGLSEPPITLFWGTPAEFLAELGRHLEMTRKEDEAPDAVEGSDD
                     WF"
BASE COUNT          545 a         1011 c         1009 g          545 t
ORIGIN      
        1 ccggttcggc aacgcgctcg ggcacaactg cgccaatggc ctgccggccc cggtcgtcgg
       61 cacggtcggt gcctgcggca cgtcgaccgc ggacaccggg atcgacgtat tttggcgatc
      121 cgacgcgccg ggcgacggac aggcggccgc cgacaccagc atcgccaacg cggacgcgcg
      181 cagcagcgcc gcgctcgagc tgccgcccga cgcggcgatc acccatgcgt acctgttctg
      241 ggggcgccag ccgggactcg gcgtctgcga cacctcgcag gaccccaacg tgtgcgtcgg
      301 ctgcctcgac gacggccatt gccaggcccc acgccgacct gcgacccggt cacgcacacc
      361 tgcacgtgcg tgccctccgg ccccgagctg tgcgacggcc tcgacaacga ctgcgacgag
      421 caggtcgacg acgagtgcat ctgcagcgcc gacgccgact gcggcggggc ggactcgggc
      481 aaggtctgcg acgccagcga caagtgcacc gatggctgcc atgcgaacgg caacggctgc
      541 cccgagggcg aggtctgcac ctcggagtcc gacgagatcg gcatgtgcgt gccggccggg
      601 ggcaccagcg atacgggcgc caccgagggc gacccgagct ccgggaccgg accggtgacg
      661 ggatcgggca acagtgacag cgccacgggc gaccccgacg gcctgtcgac cggcgacgtc
      721 ccgaccagcg gtgccaccga cgtcggcgcc agcgagacca cggacgcaga tccaggcacc
      781 ggcgagaccg gcggccagga cagcggcgag ggcggctgcg gctgcgacgt ccgcgacccc
      841 gcgcgaacgg gctgctgctc ggcctgctcg gtctgctcgg acctggcggc ggcgccgtcc
      901 cgggtgaggc cgcggccagg accgggccct cgccgaatcg tcacatcagc ccgacgagcc
      961 gacgccggat cgtgacgcgc ctgaacccgt ccgcgtcgat cttccggcca gaagaagcga
     1021 tgttttggca aaacatgtcg atccggtgga gcctgttgga tcgatctcct cactgacttt
     1081 acgttcgagg tcaccttcgc ggacgggcac gtcggagcgg tattacgggt cggtatccac
     1141 gagcactcgc acttcgcgga aatagtcccc gccggccgac tccggcacgt ccttccagac
     1201 aagatggcga ccttctcagc tcatacagca tcgccaccgc ccgtgagccg agctcccgcc
     1261 ggctcacgag cttcaagtcg atacgcttcg atagcctcgc gagcaacgtc ggcccgtggc
     1321 ccctggtatc ggggtcgcca cgcgggcgtc gttgcggagc ttccctgtcg gcggtatcgt
     1381 ccgaacgagg acttcgcatc agcgacgatg cgcatgattc cccggatgcg ccccgtcgat
     1441 cagtctgccg gcctcgagac gagcagcgac cccgaccctc gacacacggc gccaggcttc
     1501 gtgtacgacc tccgcgtctg tttgaagaag tacgcccggg ccggttgcat cttcaactat
     1561 gcccttcgtg aaagaccaca aatcgctggc gggcggtgac cctggggggt cggctgcaga
     1621 ttcgcgtatg ttcgttatcg cccttgaggt cgacgtatgg agagtcgctt tcttgtcagg
     1681 ggtttggtaa ttttcctcct gatgaacttc gaagctatcc aggagacgtt tctgagcttg
     1741 ttcgtcaatt caagtgatct ccggctgtac gtgctactga agtatccgga gctcgatcat
     1801 gatctgcccc tggaagggcc ctcgacccgt cagtattcgg ttgcggtcct ccagctgttg
     1861 agtcgacgcg gttatctcga tgtcgcgttc ttcgccggct tgatctcccg ctttccgcgg
     1921 cgcgccatcg acatacgttc cgctgcgaag ctgttgctcg gcggcgaggt cgaggtattg
     1981 ctgggcgaga acctcccgga ggcgcgggcc gcgagcctga ggccggatca ggacccgaag
     2041 accgatagtc cgctgtccca atggaagacc ctgtgccaat gtattcatca aggtcgcgtc
     2101 ctgccgatta ttggccctga cctcgatgag gggctctttg gagggacggg ggcactggcg
     2161 acgcgcttgg cgacgaagta tgcgcttata aagtcggatc atgagcgcgc tgacctcgca
     2221 aaggtcgccc agttcctcag cgtggagagg ggccgcgatt ttgcgcacaa ggccgtgcag
     2281 gcggagctca tcaacgcgct gatcgaacat cagggcgcga gcataggcga gcaagtccac
     2341 gagctgccga agctactgga cagcatcgtt gagtaccgtc gggctcgctt ggacgatcca
     2401 tacgccgctg tcgcgaaatt gcccgcgtca atatttatca ccgcgagccc cgaaacgctt
     2461 ctattcaaga cggtcaaatc cgtcgggaag aacccggaag cgctcgtttg ccggtggcgc
     2521 tcgagtggaa ataacatacc gagagagccg cgcccgaaac agccgccagg accagagaca
     2581 ccgctcgttc atcacatctt cggcgtcttc ggtgtccctg aatcactggt gttcaccgag
     2641 gatgatctct acgactttct gatcgccacc tctacgtaca agctgattcc cgcgagcgtg
     2701 cggggatcgt tgacagacag cgccctcctg ttcctcggat tccgcctcga cgactggaca
     2761 tttcgtgtgc tgttccgcat gatcatgaac ctggagggct cggcggcgat gcgcaaatat
     2821 tcccatgtcg gcgtacaggt cacgccaggg gagaatagcc gcgcggatgt ggagcgggcc
     2881 cggcgccatc tggagcggta ttttggcagc gatcggggtg cagggctgag tgagccgcca
     2941 atcacgctgt tttgggggac cccggcggaa ttcctcgcgg agctggggcg tcacttggag
     3001 atgacccgca aggaggacga ggcaccggat gccgtggaag gatccgatga ctggttctaa
     3061 ttcgtcgcag aacccgtatg tcggtccgcg tcccttcgag atcggtgaac
//
LOCUS       DASWNJ010000833         3456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_120816, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000833 DASWNJ010000000
VERSION     DASWNJ010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120816"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VGB85_14135"
     CDS             <1..389
                     /locus_tag="VGB85_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135222.1"
                     /translation="RGSGRSATALAALPATPIDAGPADVAALIGALIHVHFLRVLEFG
                     ARLGVAPSSRDRPVQMFLALPLLLPNLVGVLGTPLPSDPRAGFDGQVQRRWTEYVEHL
                     GELIPKTTVENLVATLVPRVVKAASA"
     gene            386..1570
                     /locus_tag="VGB85_14140"
     CDS             386..1570
                     /locus_tag="VGB85_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0135223.1"
                     /translation="MSVTTAPSIPDLARRLQDGARQLEQQFLGKEEIIRLLFISALAG
                     EHLVMVGPPGTAKSAIVRAFAQVIDAVYFDYLLTRFTEPNEIFGPVDIQAFRAGTYRR
                     RIEGMLPQAEVVFLDEIFKANSAILNSLLTLVNARRFTHGTNTVRVPLISLFAASNEV
                     PTDDALSAIFDRFLLRVRCDYLDSYHFRGLLQKGIQLETAQMADPVPAKKIATAAELL
                     SVQRRFGDLMRMSEEFLATFKGLVFQIRSEGIGLSDRRVVKLLKLFAASAVFDGRDAV
                     TDADFFILRHVWNTPEQEEILQEIVGPVLDRWYESHPEHSRIGATPTSLQDLLEELRV
                     IRETLTGSQVLSDMQLFSQLRNLGDIKAALTRMQDNPAAQRMVGEVDKLLETMFASSR
                     FV"
     gene            1652..3259
                     /locus_tag="VGB85_14145"
     CDS             1652..3259
                     /locus_tag="VGB85_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135224.1"
                     /translation="MADPRDGQGGPRSGGQGGQGGGLSPPGTRASRGAPRSTVNAAEL
                     ERQVGSLALSQIEGAGGLAMMRALGWQGILARLGYAVPLVVLHDLGCLIVGLGGPGAV
                     PRGIAEPELCESWRQLLVELAESDLVRAQAGWKHRDAMVGVVLARILAGVVPQLPDDI
                     RVLRPIELPLDAVHYARIEPSTAFTRYDQRQSLAWLHTLTSHRLLPLLAVEQIDVDAL
                     RLFGLFRGGPGEAAAGVDLADLYNVMISPALADVIDFSMELLPSILEVKRDAGHQVFS
                     IDGYASIERRGGIDDLELSQLALDEDVFEQKYVDDELFYFTHEKQMENEQRTHYVLVD
                     GSASMRGVREVFARGLALALCKRLALLGENVILRFFDSRLYEGVKVGAQGHAEVPYVL
                     QFRAEHGRNYARVIRQLNAELAAPRRGDGKVLVYLLTHGECQLPEHEVVALAARAPIY
                     GVFILPSGALDLGYLHHLYRVHVIDDHALSHGKRAQKARQIISDVEADYLASRRAGQQ
                     RKRAPAPGSKPGPTNMSQAPGRVRRPR"
     gene            3256..>3456
                     /locus_tag="VGB85_14150"
     CDS             3256..>3456
                     /locus_tag="VGB85_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135225.1"
                     /translation="MMDTNVLWIRAEGALRAGRHADALPWLRQLIQVVERIDFEYEEW
                     LRAFAESLRAGGQWGEAMACLAY"
BASE COUNT          511 a         1225 c         1173 g          547 t
ORIGIN      
        1 gccgcggttc gggacgttcg gccacggcgc tggcggccct gccggcgacg ccgatcgacg
       61 ccggtccggc cgacgtcgcg gccctgatcg gcgccctgat ccacgtccat ttcttgcggg
      121 tgctcgagtt cggggcccgg ctcggggtcg cgccgagctc gcgtgacagg cccgtgcaga
      181 tgttcctggc gctgcccctg ctgctgccca acctggtcgg cgtgctcggc acaccgctgc
      241 ccagcgaccc gcgcgccggc ttcgacgggc aggtgcagcg gcgctggacc gagtacgtcg
      301 agcacctcgg cgagctgatc ccaaaaacta cagtagaaaa tctagtggcc accttggtcc
      361 cccgcgtcgt aaaagcagca tcggcatgag cgtgaccacg gcgccttcga tccccgacct
      421 cgcccggcgc ttgcaggacg gcgcgcgcca gctggagcag cagttcctgg gcaaggagga
      481 gatcattcgg ctcctcttca tcagcgccct cgcgggcgag cacctggtga tggtcggccc
      541 gcccggcacc gccaagagcg cgatcgtccg cgccttcgcg caggtgatcg acgccgtgta
      601 cttcgattac ctgctgacgc gcttcaccga gcccaacgaa attttcgggc ccgtcgatat
      661 ccaggcgttt cgcgccggca cgtaccgccg ccggatcgag ggcatgctgc cgcaggccga
      721 ggtcgtcttc ctcgacgaga tcttcaaggc caacagcgcg atcctcaact cgctgctgac
      781 cctcgtcaac gcgcgccgct tcacccacgg caccaacacc gtgcgcgtgc ccctgatctc
      841 gctgttcgcg gcctccaacg aggtgcccac cgacgacgcg ctctcggcga tcttcgaccg
      901 cttcctgctg cgcgtccgct gcgactacct cgacagctat cactttcgcg gcctgctgca
      961 aaaaggcatc cagctcgaga cggcccagat ggccgatccg gtgcccgcca agaagatcgc
     1021 caccgccgcc gagttgttgt cggtgcagcg ccggttcggc gacctgatgc gcatgagcga
     1081 ggagttcctt gcgaccttca agggcctcgt gttccagatc cgcagcgagg gcatcggcct
     1141 cagcgaccgt cgcgtcgtca agctgctcaa gctgttcgcc gcctcggccg tgttcgacgg
     1201 ccgtgatgcg gttactgatg cggatttttt catcctgcgc cacgtgtgga acacgcccga
     1261 gcaggaggag atcctgcaag agatcgtcgg ccccgtgctc gatcgctggt acgagagcca
     1321 ccccgagcac agccggatcg gcgcgacccc gaccagcctg caagacctgc tcgaggagct
     1381 ccgcgtcatc cgcgagaccc tcacaggatc gcaggtgctc tcggacatgc agctgttctc
     1441 gcagctgcgc aacctcggcg acatcaaggc cgccctcaca cgcatgcagg acaacccggc
     1501 cgcccagcgc atggtcggcg aggtcgacaa gctgctcgag accatgttcg cctcgagccg
     1561 cttcgtctag tcgtcgaggt cgcatgggcg gtgacacgcg aatcgctcgg cgctctcccg
     1621 tccgcggtcc cgcgcggagg atcatttaaa gatggccgac ccacgtgatg ggcagggcgg
     1681 cccgcgatcg ggcggtcagg gcgggcaggg cggcgggctg agtccgccgg gcacccgtgc
     1741 gtcgcgtggg gcgccgcgca gcaccgtcaa cgcggcggag ctcgagcggc aggtcggtag
     1801 cctggcgctg tcgcagatcg aaggcgccgg cgggctggcg atgatgcgcg cgctcggctg
     1861 gcaggggatc ctcgcgcgcc tcgggtatgc cgtgccgctg gtggtcctgc acgacctcgg
     1921 gtgcctgatc gtcgggctcg gcgggccagg ggccgtgccg cgcgggatcg ccgagccgga
     1981 gctgtgcgag tcgtggcggc agctgctggt cgagctcgcg gagtcggacc tcgtgcgcgc
     2041 gcaggcgggc tggaagcacc gcgacgcgat ggtcggcgtc gtgctcgcgc ggatcctcgc
     2101 cggcgtggtg ccgcagctcc cggacgacat ccgcgtgctg cggccgatcg agctcccgct
     2161 cgacgccgtc cactacgcgc gcatcgagcc gagcaccgcc tttacgcgct acgaccagcg
     2221 gcagtcgctc gcgtggctgc acacgctgac cagccaccga ttactcccct tgctggccgt
     2281 cgagcagatc gacgtcgatg cattgcgcct gttcggtctg ttccgcggcg ggcccggcga
     2341 ggcggcggcc ggcgtcgatc tcgcggacct gtacaatgtc atgatcagcc ccgcgctggc
     2401 ggacgtgatc gacttctcga tggagctgct gccgtcgatc ctcgaggtca agcgcgacgc
     2461 cggccaccag gtgttctcga tcgatggata tgcgtcgatc gagcgccgcg gcggcatcga
     2521 cgacctcgag ctgtcgcagc tggccctcga cgaggacgtc ttcgagcaga agtacgtcga
     2581 cgacgagctg ttctacttca cgcacgagaa gcagatggag aacgagcagc gcacgcacta
     2641 cgtgctcgtc gacggctccg cctcgatgcg cggcgtgcga gaggtgttcg cgcgcggcct
     2701 ggcgctcgcg ctgtgcaagc ggctcgcgct gctcggcgag aacgtgatcc tgcgcttctt
     2761 cgacagccgg ctgtacgagg gcgtcaaggt cggcgcgcag ggccacgccg aggtgcccta
     2821 cgtgctgcag tttcgggccg agcacggtcg caactacgcc cgcgtgatcc gccagctcaa
     2881 cgccgagctc gccgcgccgc gccgcgggga cggcaaggtg ctcgtgtacc tgctgaccca
     2941 cggcgagtgc cagctgcccg agcacgaggt cgtcgcgctg gccgcacgcg cgccgatcta
     3001 cggcgtgttc atcctgccca gcggcgcgct cgacctcggc tacctgcacc acctttaccg
     3061 cgtgcacgtg atcgacgacc acgcgctcag ccacggcaag cgggcccaga aggcgcgcca
     3121 gatcatcagc gacgtcgagg ccgactacct cgcgtcgcgg cgcgccggac agcagcgcaa
     3181 gcgcgctcct gcacctggtt ccaagcctgg cccgacgaac atgtcgcaag cgccaggtcg
     3241 cgtccggagg ccgcgatgat ggacaccaac gtgctgtgga tccgcgccga gggggcgctg
     3301 cgggccggtc gccacgccga cgccctgccg tggctgcgcc agctgatcca ggtcgtcgag
     3361 cgcatcgact tcgagtacga ggagtggctg cgcgccttcg ccgagtcgct gcgcgcgggc
     3421 gggcagtggg gcgaggcgat ggcctgcctc gcctac
//
LOCUS       DASWNJ010000834        11791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_367829, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000834 DASWNJ010000000
VERSION     DASWNJ010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 11791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11791
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367829"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VGB85_14155"
     CDS             complement(<1..643)
                     /locus_tag="VGB85_14155"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0135226.1"
                     /translation="MSLAEVRRRLPVVASLPKPDNRWTSHESRKAAAAPRWAVWELTL
                     ACDQKCSHCGPRAGHRRSDELSTEECLKVVQELKELGAGEVVLIGGEAYLRNDFILII
                     RAIREAGMSCTMTTGGYNLNRERIDAMIEAGIGSITFSIDGLQETHDRLRGMPNSWAR
                     AFQAMRDVKAAGGKIACNSQINALTRGELLPLFELLADEGIHSWQLQITVPHGN"
     gene            complement(746..1522)
                     /locus_tag="VGB85_14160"
     CDS             complement(746..1522)
                     /locus_tag="VGB85_14160"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY0135227.1"
                     /translation="MNFSGASEITESDAGLRWQVPDGWQQGRGAFGGLVLATLLRAIE
                     RAEPDRVRTVRSLTGDLCGPVLPGPAALTVEVLRRGNNLSNIDARLVQDGQVQARASA
                     VLSAPRAHATLRRPPVAPEAPAWRDLPDLGLGPPVGPAFAPHFEYRSAIDFTTADGSE
                     TLGWLRLRRPPATLDAAAVVAMLDAWWPAIFSGGPRPMATVSFTAELLGDPAELAPDV
                     PLLYRGRVIALHEGFCVEFRELWDRHRLVAMNQQTFAILR"
     gene            complement(1530..2168)
                     /locus_tag="VGB85_14165"
     CDS             complement(1530..2168)
                     /locus_tag="VGB85_14165"
                     /inference="COORDINATES: protein motif:HMM:NF013298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY0135228.1"
                     /translation="MQSQPTIAILGAGNVGGNLGVRLAASGFAVKFGVRAGSDVKDLL
                     SRAGARAELLSPQAAAAAADIVFIATPSAVAVQVAQESELTGKIVVDCTNPLTWNDGP
                     VWSPPAAGSVAAAIAQAVPGARVIKGFNTFGAEIHLDPSLGGTVADVFLAGDDAEAKA
                     AVASLAEAAGFTPVDSGPLRNAAVLENLAILWIHLALREGHGRSWAFKKVTR"
     gene            2286..2879
                     /gene="wrbA"
                     /locus_tag="VGB85_14170"
     CDS             2286..2879
                     /gene="wrbA"
                     /locus_tag="VGB85_14170"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF002999.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEY0135229.1"
                     /translation="MTKVAVIYYSSTGTNHATAQAVAEGATGAGAEVRVRLVAETAPE
                     AAVASNPAWKAFTERSADEPRATLADLEWADAIVFGTPTRFGNVAAQLKSFLDGAGGL
                     WFTGKLANKVYAAFTSAQNPHGGQESTLLALYNSIYHFGGYIVTPGYTDPSVFASGGN
                     PYGVSVTATGSGPTDADLAHARYLGKRVAEVAQKLAR"
     gene            3096..3491
                     /locus_tag="VGB85_14175"
     CDS             3096..3491
                     /locus_tag="VGB85_14175"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0135230.1"
                     /translation="MRRVCEHLCPRFQLAMDVISRPWNGLIMASLEDGPVRFGELLER
                     IEHVGDRMLSARLKDLQARGLVERRVIPGPPVRVEYELTDAGRGFQAVFEAIARWGQT
                     LAEPPRVRARTAGGRVAKITKTTKLAKRR"
     gene            complement(3501..3692)
                     /locus_tag="VGB85_14180"
     CDS             complement(3501..3692)
                     /locus_tag="VGB85_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135231.1"
                     /translation="MALLTWLALWPIVTLMMCTVRPLLAELPIPLQTLIMTAIIVPLM
                     TWALMPMLTRVFRGWLVPR"
     gene            complement(3868..5295)
                     /locus_tag="VGB85_14185"
     CDS             complement(3868..5295)
                     /locus_tag="VGB85_14185"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0135232.1"
                     /translation="MTATVEGEWGSTFPGGQIDAGTTSPGPMAITNIGPIDALGEPRP
                     NTQPPGMITTAMGTLHEGGKSSRATALTPGTLIDGKYRLEALLGRGGMGIVWRAEHAH
                     LHGSIAIKFLLDRFRAKPQVIERFRQEATVMGELGHPNIVRVYDISSASSDMPYLTME
                     LLQQGSLREYLKRSGGKLPPDEAVELMDGVLSALIAAHKRGIVHRDIKPDNLMLAAVR
                     SFETDMNEVQLKILDFGASLLLADTSDINKGEGLMGTPYYMSPEQASGAALDQRTDLY
                     SAAVVLYEAISGKLPHMADDVHSLVYSIATEEPTPISEHAPNLARPYREFFDRALTRD
                     PLRRFQTAEDMRQAMRGLSPKLAGKNRNTALYLASGDTGPIQLAHAAASRPDTQPLRS
                     FRDVLEGPARSGQGAIPTPNPAPLPTLAPRLVAASMAAIVPALAIQWARFGSPAQAPA
                     LDTVLIWAVCAAAASVLAWRLGPRG"
     gene            complement(5557..6840)
                     /locus_tag="VGB85_14190"
     CDS             complement(5557..6840)
                     /locus_tag="VGB85_14190"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HEY0135233.1"
                     /translation="MPSILLLPIACGGDDDAGGSGETPTTKPTPDPSTTTDVPTTGGD
                     SETLVPTTGAPDLTTTTDEPDTTTTGEQPDPLTVDCGAPPAGAKAAQYNHQPSVMGGT
                     PAYTWNATGLPDGLTIDAGSGKISGQPQTPGDYTFELTVTDSGGMSAMTTCPSVNINE
                     QLGVDLGAMLGPCITEDESLLDYIVGGDGSPIECVAPKGIGDGFLPAGVTVDKAKCTQ
                     QGTITEDRYGTYAWIVRARQSGVEVFAPYCATQDVQAANAYTIVGNHSGGVDNELEPL
                     VLDHKAGQPLLLDGDADPSFIVDKGACGASCFFGFLYQVSPSPFGTGACTTDKDKCYG
                     LCPLIADPNEPDGDKQIGCSLVPNMGLKTGFAHEMWAKGDVTPAEFQTRPFILQWQID
                     YCITNVKGECTGKDNILANGADSNLAFAVIVRPQP"
     gene            complement(7090..7527)
                     /locus_tag="VGB85_14195"
     CDS             complement(7090..7527)
                     /locus_tag="VGB85_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEY0135234.1"
                     /translation="MTDEASEAAPPPQAAAPTVNGDMAALDSVRVDKWLWAARCFKTR
                     GLASEACGAGHVKLNGTSVKSAKLVRRGDELTVRLGDELKIFAITGLSDRRGPAPVAR
                     TLYEDKSPPKPEAADVPGMLVRDRGLGRPTKRELRDIRRVRGY"
     gene            complement(7524..8255)
                     /locus_tag="VGB85_14200"
     CDS             complement(7524..8255)
                     /locus_tag="VGB85_14200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0135235.1"
                     /translation="MLELCPRLAAIAELVLPGRPLADIGTDHGRLPAALVLRGVVPRA
                     FACDRAAGPVARARATVTRNGIADRVAVRSGEGLAPLAIGEADTVVVAGMGAETIVRI
                     LAGHPDRTRALRRLVLQPNFGHELLRRWLVAHHFMLVDERLTLDRGRFYTVIAAEPAA
                     QDATNWRDWDDAQWAIGPFVLRRGGPLLVQHLTAELRLREAEAAGLAQASAPDPARVA
                     AATARVALLADALAKATALAHPGSA"
     gene            8396..9739
                     /locus_tag="VGB85_14205"
     CDS             8396..9739
                     /locus_tag="VGB85_14205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0135236.1"
                     /translation="MTESDLAAASRTGEAAAAEQMAAAARGDIEAFLRLCESYTPMLM
                     GVALRIVGDRNDAEKAVHEVLVEAWRSCRVFDPARFSVRVWWTIGVRTMALRRSQGRR
                     VKRMASTHDTMIAQLGPAHESGDVPALRAKVRRVLDSLSSEQRSLLQLAYFDGLSPVE
                     IGDRSTMNANQVRIQVCEALRCLRTGLFWIDAPRVGPADELAAGYLLDEVDPGERSSL
                     ELGDPVRGLDAAAAAIVRDTVHALALYTMPGPAPPRIKARVNAAITGPDRMQPFAVDL
                     ARFFSVGLEAARDLIGRIDRPHGWLDDASGVRLLSVTPGEPEPAGVSDMSQKPEAYEA
                     TLVQLPAWPAACLVRVAPGQSLGARYPGTGASALVLQGAVLGDDGGNARAGELWTWPP
                     GAALGTASPGEVELICAVRAGAPARAHDGHGHDGHARDGHPRDGHANAGTVDSQA"
     gene            9799..10095
                     /locus_tag="VGB85_14210"
     CDS             9799..10095
                     /locus_tag="VGB85_14210"
                     /inference="COORDINATES: protein motif:HMM:NF016262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HEY0135237.1"
                     /translation="MSADTTRKAPARESLLAVHQFPGEYVIKAFGPGHDEFHVAVVAA
                     AHAELHPERVAVQTRVTNSGGRQCVTLTLHALTVEEVEAVYERLHLLETLQLIL"
     gene            10191..10838
                     /locus_tag="VGB85_14215"
     CDS             10191..10838
                     /locus_tag="VGB85_14215"
                     /inference="COORDINATES: protein motif:HMM:NF024436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135238.1"
                     /translation="MSTRPRFLPRLQSRPLALLLALALVPAAFTSAGCKPKAVRGGPG
                     TENPNLDQGAMSTTLDRRDLQELMQTNMAKMMESPWWAQMSGTPGQIVAIWPIKNETS
                     EHIDDQLNTLLADLETELVNSGVVSVVSRERQAEMANEVAVQQNRSIYDPAFANQISR
                     QLGARFYITGKVGSVDEFFNKERRVQYTLFIQVIEVETSLVRFQYKSERSKAIVR"
     gene            10860..>11791
                     /locus_tag="VGB85_14220"
     CDS             10860..>11791
                     /locus_tag="VGB85_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135239.1"
                     /translation="MSSPIQRTPRPRRRGLLLAVAGLMACATYSEKTQLARDAAQTGN
                     LQASEKVLNKFLGVRRSADLPTEWKKETIVALLERAMVLHGKGDWKLSARDLVAADKQ
                     LVLLDVARDAGQVGKWVFSDSSGKYHAPPSEKLALNAFNMLNYLLADDLSGARVEAKR
                     FTVTREYLGQLDPLDQAAHGAFGSYVAGFVFERLGETETALRYYDEALQEQPFPSLRA
                     PVARLAARSSYRTPRIRALLGEPEASASRPTNTLLAMQAPEPEAPAAAPAAPPPAGEL
                     LVVMNVGRVPYKVPERMPIGLAVGLGATYVTGDTS"
BASE COUNT         1670 a         4178 c         4242 g         1701 t
ORIGIN      
        1 cgttgccgtg cggcaccgtg atctgcagct gccaggagtg gatgccctcg tcggcgagca
       61 gctcgaacag cggcagcagc tcaccgcggg tcagcgcgtt gatctgcgag ttgcaggcga
      121 tcttgccgcc ggcggccttg acgtcgcgca tcgcctggaa cgcgcgcgcc cagctgttcg
      181 gcatgccgcg caggcggtcg tgcgtctcct gcaggccgtc gatcgagaag gtgatgctgc
      241 cgatgccggc ctcgatcatc gcgtcgatgc gctcgcggtt gaggttgtag ccgccggtcg
      301 tcatcgtgca gctcatgccc gcctcgcgga tcgcacgaat gatcaggatg aagtcgttgc
      361 gcaggtaggc ctcgccgccg atcagcacga cctcgccggc gcccagctcc ttgagctcct
      421 gcacgacctt gaggcactcc tcggtgctca gctcgtcgct gcgccggtgc cccgcccgcg
      481 gcccgcagtg cgaacacttc tgatcgcagg cgagcgtcag ctcccacacc gcccagcgcg
      541 gcgccgcggc ggccttgcgc gactcgtggc tggtccagcg attatcgggt ttgggcaagc
      601 tggcgaccac cggcaagcga cgtcgaactt cagccaagga catagcgaga cgctatcacg
      661 tccgcatcgg ccggcccagc cgcgcgtatc catgcccgat tccgtcacct gtcccatggg
      721 acagcatcat ggggcggtgg cgaggctagc gcaggatcgc aaaggtctgc tggttcatgg
      781 cgacgagcct gtggcggtcc cacagctcgc ggaactcgac gcaaaagccc tcgtggagcg
      841 cgatcacgcg gccgcgatac agcagcggca catccggcgc gagctcggcg gggtcgccga
      901 ggagctcggc ggtgaacgag acggtcgcca tcggtctcgg gccgccggag aagatcgcgg
      961 gccaccaggc gtcgagcatc gccaccacgg ccgcggcgtc gagcgtcgcg ggcggccgtc
     1021 gcaggcgcag ccaccccagc gtctcgctgc cgtcggcggt cgtgaagtcg atcgcggagc
     1081 gatactcgaa gtgtggagcg aaggccgggc cgaccggcgg gccgaggccg aggtccggga
     1141 ggtcgcgcca cgccggcgcc tcgggggcca cgggcgggcg acgcagggtc gcgtgggccc
     1201 tgggcgcgct gagcacggcg ctcgcgcgcg cctggacctg gccgtcctgc acgagccgcg
     1261 cgtcgatgtt gctgaggttg ttgccgcgcc gcaggacctc gacggtcagc gcggccgggc
     1321 ccggcagcac ggggccgcac aggtcgccgg tcagcgaccg cacggtccgc acgcggtccg
     1381 gctcggcgcg ttcgatcgcg cgcagcaggg tggcgagcac gaggccgccg aacgcccccc
     1441 tgccctgctg ccagccgtcg gggacctgcc agcgcaggcc cgcgtcgctc tccgtgatct
     1501 cgctggcccc ggaaaaattc acgggcgctc tagcgggtga cctttttgaa cgcccagctg
     1561 cggccgtggc cctcgcgcag cgcgaggtgg atccacagga tcgcgaggtt ctcgagcacg
     1621 gcggcgttgc gcagcgggcc gctgtcgacg ggcgtgaaac cggcggcctc ggccagcgag
     1681 gcgaccgcgg ccttggcctc ggcgtcgtcg cccgcgagga acacatcggc cacggtgccg
     1741 ccgagggagg ggtcgaggtg gatctcggcg ccgaaggtgt tgaagccctt gatgacgcgc
     1801 gcgcccggca cggcctgggc gatcgcggcg gcgaccgaac ccgcggccgg gggcgaccac
     1861 accgggccgt cgttccaggt cagcgggttg gtgcagtcga cgacgatctt gcccgtcagc
     1921 tcggactcct gggcgacctg cacggcgacc gcggagggcg tggcgatgaa cacgatatcg
     1981 gccgcggccg ccgcggcttg cggcgagagc agctcggcgc gcgcgcccgc gcgcgacagc
     2041 agatccttga cgtcgctgcc ggcacgcacg ccgaatttga cggcgaagcc ggacgccgcg
     2101 aggcgcacgc ccaggttacc gccgacgttg cccgccccga ggatcgcgat cgttggttgg
     2161 ctttgcatcg agccaatgtc taacacggtc gccgtccgcc ggaccacggg gcctgtggcg
     2221 gcgagcaaca ctgtgttgca ggtatggacc tgccgggccc cgcgacgccg cgttaccgtg
     2281 ccgccatgac gaaggtcgca gtcatttact actcatccac ggggaccaac catgcgacgg
     2341 cgcaggccgt cgccgagggc gccaccggcg ctggcgcgga ggtccgcgtc cgtctcgtcg
     2401 ccgagaccgc gccggaggcc gccgtcgcca gcaacccggc ctggaaggcg ttcaccgagc
     2461 gcagcgccga cgagccgcgg gccacgctgg ccgacctcga gtgggccgac gcgatcgtgt
     2521 tcggcacgcc gacccgcttc ggcaacgtcg ccgcccagct caagtcgttc ctcgacggcg
     2581 ccggtgggct gtggttcacg ggcaagctgg ccaacaaggt ctacgccgct tttacctccg
     2641 cgcagaaccc tcatggcggc caggagtcga ccctgctggc gctctacaac tcgatctacc
     2701 acttcggcgg gtacatcgtg acccccggct acaccgaccc gtccgtgttc gcctccggcg
     2761 gcaaccccta cggcgtctcg gtcaccgcga ccggcagcgg ccccaccgac gccgacctcg
     2821 cccacgcgcg ctacctgggc aagcgggtcg ccgaggtggc gcagaagctc gcgcgctaac
     2881 acggcgtaca gcggggcgcg ccggttttgc gccccgcgcc cgaactcgcg gccttcgtcg
     2941 tcccggtctc ttcgcttgcg cggcatgggc tgcctccgat cgagcgcctg cttccgaaaa
     3001 ggaagcgagc ggggaatgag aagaaccttg cgcggggcct ccaagtttgt caagccagtg
     3061 cgcccgcggg gccctgcggc tatactccac gggcgatgcg gcgcgtctgt gagcacctgt
     3121 gcccgaggtt ccagctggcg atggacgtca tctcccggcc gtggaacggg ctgatcatgg
     3181 cctccctcga ggacggcccg gtccgcttcg gcgagctgct ggagcgcatc gaacacgtcg
     3241 gcgaccgcat gctctcggcg cgcctgaagg acctgcaggc gcgcggcctg gtcgagcggc
     3301 gggtgatccc ggggccgccc gtgcgcgtcg agtacgagct gaccgacgcc gggcgcgggt
     3361 tccaggccgt gttcgaggcg atcgcccgct ggggtcagac gctcgccgag cctcctcgtg
     3421 tccgggcgcg gaccgccggt ggccgggtcg cgaagattac gaagaccacg aagctcgcga
     3481 agcggcgctg agatcagcgc tcagcgaggc accagccacc cgcggaacac acgggtgagc
     3541 atcggcatca gcgcccaggt catcagcggc acgatgatcg ccgtcatgat gagggtttgc
     3601 agcgggatcg gcagctccgc gagcaacggt cgcaccgtgc acatcatcag agtgacgatg
     3661 ggccacaggg cgagccaggt gagcagcgcc atcttccaga ccggcgaacg gtgcacggga
     3721 acctcgttct gaagatatct gggtcgcgtc cgcgacacca tctcattgct tgcagacggt
     3781 atgtatcgac ctacctcgcg cgtcggtcga atgtccggcg tgtcgtcatg ggtccatgcg
     3841 gatgcatggg gccgcatgac ggcaacctca gccgcgcggg ccgaggcgcc acgcgagcac
     3901 gctggctgcg gccgcgcaga cggcccagat caacacggta tcgagcgcgg gcgcctgcgc
     3961 gggcgagccg aagcgggccc actggatcgc cagcgcgggg acgatcgcgg ccatgctcgc
     4021 ggcgaccagg cgcggcgcga gggtcggcag cggcgcgggg ttcggcgtcg ggatggcgcc
     4081 ctgaccggag cgcgccgggc cctcgagcac gtcgcggaac gaccgcagcg gctgggtgtc
     4141 ggggcgcgag gcggcggcgt gggcgagctg gatcgggccg gtgtcacccg aggcgaggta
     4201 gagggcggtg ttgcggttct tgccggcgag cttgggcgac aggccgcgca tcgcctggcg
     4261 catgtcctcg gcggtctgga accggcgcag cgggtcgcgg gtcagcgcgc ggtcgaagaa
     4321 ctcgcggtac ggtcgcgcca ggttcggcgc gtgctccgag atcggcgtcg gctcctcggt
     4381 ggcgatgctg tagaccagcg agtgcacgtc gtcggccatg tgcggcagct tgccggagat
     4441 ggcctcgtag agcacgaccg cggcggagta gaggtcggtg cgctgatcga gggccgcgcc
     4501 ggaggcctgc tccggggaca tgtaataggg cgtgcccatc aggccctcgc ccttgttgat
     4561 gtcgctggtg tcggcgagca gcagcgaggc gccgaagtcg aggatcttga gctggacctc
     4621 gttcatgtcg gtctcgaacg agcggacggc cgccagcatc aggttatccg gcttgatgtc
     4681 gcggtgcacg atgccccgct tgtgcgcggc gatcagcgcc gacagtacgc cgtccatcag
     4741 ctcgaccgcc tcgtcgggcg gcagcttgcc gccgctgcgc ttgagatact cgcgcaggct
     4801 gccctgctgc agcagctcca tcgtcaggta cggcatgtcc gacgaggccg aggagatgtc
     4861 gtaaacgcgg acgatgttcg ggtggccgag ctcgcccatc accgtcgcct cctgacggaa
     4921 gcgctcgatc acctgcggct tggcccggaa ccggtcgagc aggaacttga tcgcgatgct
     4981 gccgtgcagg tgggcgtgct cggcccgcca gacgatgccc atgccgccgc ggccgagcag
     5041 cgcctcgagg cggtacttgc cgtcgatcag ggtgcccggc gtcagggcgg tggcccgcga
     5101 gctcttgccg ccctcgtgca gcgtgcccat cgccgtggtg atcatgccgg gcggctgcgt
     5161 gttcgggcgc ggctcgccga gcgcatcgat cggtccgatg ttggtgatcg ccatcgggcc
     5221 gggcgaggtg gtgccggcgt cgatctgccc gccggggaag gtcgagcccc actcgccctc
     5281 gacggtggcg gtcatctccg aatgggtgcg accggccgtc gatgtcgacg cgcccgcgga
     5341 cgccgaccgc tggtcgggcg cgctctgtct atcggtcgca ggggtcaatg gcgtggctac
     5401 cgggcggggt cggcgattgt atcgcggtcg tggcggcgag cggcgtcgcc tgggccacac
     5461 accgcgaagg cgagaaggca gtaaggcgag aaagcagtaa ggcgagaaag caggaaggcg
     5521 cgaagccgcg ggcgcacccg cggcggatga cggagtctac ggctgcgggc ggacgatgac
     5581 ggcgaacgcg aggttcgagt cggcgccgtt ggcgaggatg ttgtccttgc cggtgcactc
     5641 gcccttgacg ttcgtgatgc agtagtcgat ctgccactgc aggatgaagg ggcgggtctg
     5701 gaactcggcg ggggtcacgt cgcccttggc ccacatctcg tgcgcgaaac cggtcttgag
     5761 gcccatgttc gggaccagcg agcagccgat ctgcttgtcg ccgtcgggct cgttggggtc
     5821 ggcgatcagc gggcacaggc cgtagcactt gtccttgtcg gtcgtgcacg cgccggtgcc
     5881 gaagggcgac ggggagacct gatacaggaa gccgaagaag cacgaggctc cgcacgcgcc
     5941 cttgtcgacg atgaagctcg ggtcggcgtc gccgtcgagc agcagcggct ggccggcctt
     6001 gtgatcgagg acgagcggct cgagctcgtt gtcgacgccg ccgctgtgat tgccgacgat
     6061 cgtgtaggcg ttggcggcct ggacgtcctg ggtggcgcag tacggcgcga acacctcgac
     6121 gccggactgc cgggcgcgca cgatccacgc gtaggtgccg tagcggtcct cggtgatcgt
     6181 gccctgctgc gtgcacttgg ccttgtcgac cgtgacgccg gccggcaaaa agccgtcgcc
     6241 gatgcccttg ggcgcgacgc actcgatcgg cgatccgtcg ccgccgacga tgtagtcgag
     6301 cagcgactcg tcctcggtga tgcacggccc gagcatcgcg ccgaggtcga cgccgagctg
     6361 ctcgttgatg ttgacgctcg ggcaggtcgt catcgccgac atgccgccgc tgtccgtcac
     6421 cgtcagctcg aaggtgtagt cgcccggggt ctgcggctgg ccgctgatct tgcccgagcc
     6481 ggcgtcgatc gtcaggccgt ccggcaggcc cgtggcgttc cacgtgtagg ccggcgtgcc
     6541 gcccatgacg ctcggctggt ggttgtactg cgccgccttg gcgccggccg gcggggcccc
     6601 gcagtcgacc gtcagcgggt ccggctgctc gccggtggtc gtcgtgtccg gctcgtcggt
     6661 ggtggtcgtc aggtccggcg cgccggtggt cggcaccagc gtctcgctgt cgccgccggt
     6721 ggtcggcacg tcggtcgtgg tcgacgggtc gggcgtcggc ttggtggtcg gcgtctcgcc
     6781 gctgccgccc gcgtcgtcgt cgcccccgca ggcgatcggc agcagcagga tgctcggcat
     6841 caacagcgca cgaaggccgg acgccagcga accagggcct ccgcatgcga cggccgaatt
     6901 gtggccgcga ggggggtcgt gacggacacg catgttcaag tctcctttag ggcaacagca
     6961 gcgagttaga gggcgcaact gcgcacccga gcgcgaggat atcactccga ctcgcggcgc
     7021 tggccaacaa acttgtgacg gtcggcggcc cggtgcgccc cgacgccccc cggccggaag
     7081 gctcggggat cagtaacccc gcacgcggcg gatgtcccgc agctcgcgct tggtcggtcg
     7141 tccgaggccg cggtcgcgga ccagcatccc cgggacgtcc gcagcctcgg gcttgggcgg
     7201 ggacttgtcc tcgtagagcg tgcgggcgac cggcgccggg ccgcggcgat cgctgaggcc
     7261 cgtgatcgcg aagatcttga gctcgtcgcc gaggcgcacc gtcagctcgt cgccgcggcg
     7321 caccagcttg gccgacttca ccgaggtgcc gttgagcttg acgtggccgg cgccgcacgc
     7381 ctcgctggcg aggccccgcg tcttgaagca gcgcgccgcc cacaaccact tgtcgacccg
     7441 caccgagtcc agcgccgcca tgtctccgtt gaccgtcggc gccgccgcct ggggtggcgg
     7501 ggctgcttcg ctcgcctcgt ccgtcatgcc gacccaggat gcgccagcgc ggtcgccttg
     7561 gccagtgcat cggcgagcag ggcgacgcgg gcggtcgccg cggcgacgcg ggcggggtcc
     7621 ggggcgctgg cctgcgcgag cccggccgcc tcggcttccc gcagtcgcag ctcggccgtc
     7681 aaatgttgga ccagcagggg cccgccgcgc cggagcacga aggggccgat cgcccactgg
     7741 gcatcgtccc agtctcgcca gtttgtcgcg tcctgcgcgg ccggctcggc ggcgatcacg
     7801 gtgtagaagc ggccgcggtc gagggtcagg cgctcgtcga ccagcatgaa gtgatgggcg
     7861 acgagccagc ggcgcaggag ctcgtggccg aagttgggct ggagcaccag tcggcgcagc
     7921 gctcgcgtgc ggtcggggtg gccggcgagg atgcggacga tcgtctcggc gcccatgccg
     7981 gcgacgacga cggtgtcggc ctcgccgatc gccagcggcg cgagcccctc gccgctgcgc
     8041 acggcgacgc ggtcggcgat cccgttgcgg gtgaccgtcg cgcgcgcgcg ggcgacgggt
     8101 ccggccgcgc gatcgcacgc gaatgcgcgc ggcaccacgc cgcgcagcac cagcgccgcg
     8161 ggcagccgcc catggtcggt cccgatgtcg gcgagcggcc ggcccggcag gaccagctcg
     8221 gcgatcgcgg ccaggcgtgg gcacagctcc aacatttcgg cgcgatccct tagcacgcgc
     8281 tgggctcgcc cgcaccgctg cgcgcggcca tggacggcga tcctccgtca tcctcggccc
     8341 ggaggcaggg cggcgccttg acaagcccag gggatgcgag gaccctcatt gcaacatgac
     8401 ggagagtgac ctcgcagccg cctcacgcac gggcgaggcc gcggctgcgg agcagatggc
     8461 ggccgcggct cgcggcgaca tcgaggcgtt cctccgcctg tgcgagagct acacgccgat
     8521 gctgatgggc gtggccctgc gcatcgtcgg cgaccgcaac gacgccgaga aggccgtcca
     8581 cgaggtgctc gtcgaggcct ggcgcagttg cagggtgttc gaccccgccc gcttcagcgt
     8641 ccgcgtctgg tggacgatcg gcgtgcgcac gatggcgctg cggcgcagcc agggtcgtcg
     8701 cgtcaagcgg atggcctcga cccacgacac gatgatcgcc cagctcgggc cggcccacga
     8761 gtcgggcgac gtcccggccc tgcgcgccaa ggtccgtcgc gtgctcgact ccctcagcag
     8821 cgagcagcgc tcgctgctgc agctcgcgta cttcgacggc ctgtcgccgg tcgagatcgg
     8881 cgaccgctcg acgatgaacg ccaaccaggt gcgcatccag gtgtgcgagg cgctgcgctg
     8941 cctgcggacc ggcctgttct ggatcgacgc gccgagggtc gggcccgccg acgagctggc
     9001 cgccggttac ctcctcgacg aggtcgaccc cggcgagcgc agctcgctcg agctcggcga
     9061 cccggtccgt ggcctcgacg ccgccgccgc ggcgatcgtc cgcgacacgg tgcacgcgct
     9121 cgcgctgtac acgatgcctg gcccggcgcc gccgcggatc aaggcccgcg tcaacgcggc
     9181 gatcaccggg cccgatcgca tgcagccctt cgcggtcgac ctcgcccgct tcttctcggt
     9241 cggcctcgag gccgcgcgcg acctgatcgg tcgcatcgac cgcccgcacg gctggctcga
     9301 cgacgccagc ggcgtccgcc tgctctccgt cacgcccggg gagcccgagc cggctggcgt
     9361 ttcggacatg tcccaaaagc cagaggcgta tgaggcgacc ctcgtgcagc tgcccgcgtg
     9421 gccggccgcg tgcctcgtgc gcgtcgcccc cggccagagc ctcggcgcgc gttacccggg
     9481 caccggcgcg tccgcgctgg tgttgcaggg cgccgtgctc ggcgacgacg gcggcaacgc
     9541 ccgcgccggc gagctgtgga cgtggccgcc cggcgccgct ctcgggaccg cgagccccgg
     9601 cgaggtcgag ctgatctgcg cggtgcgagc tggcgcgccc gccagggccc acgacggtca
     9661 cgggcacgac ggccacgcga gggacggcca cccgagggac ggccacgcga acgccggcac
     9721 cgtcgactcg caagcttgac ggggttccgc gggcggtgct gcgtgctccg ggcgcgcagg
     9781 cgtgatagga ctgcccgcat gtccgccgac accacccgta aggccccggc acgggagtcc
     9841 ctgctggccg tgcatcagtt cccgggcgag tatgtgatca aggcgttcgg gcccggtcac
     9901 gacgagttcc acgtcgcggt cgtcgcggcc gcgcacgccg agctccaccc cgagcgcgtg
     9961 gccgtgcaga cccgcgtcac caactccggc ggccgccagt gcgtgaccct cacgctgcac
    10021 gccctgaccg tcgaggaggt cgaggccgtg tacgagcgac tccacctgct ggagacgctc
    10081 cagctgatcc tgtgagcgct gtcacggcca agatccgcag cacagccttc gagcgacaac
    10141 attcgccgtc ctgttcacgt ccacggggga ccgcgtcccc aggagccttc atgtcgaccc
    10201 gcccccgctt cctcccgcgc ctccagtctc gcccgcttgc cctcctcctc gccctcgccc
    10261 tcgtccccgc ggccttcacc tccgccggtt gcaagccgaa ggccgtccgc ggcggaccgg
    10321 gcaccgagaa cccgaacctg gaccagggcg cgatgagcac cacgctcgac cgccgcgacc
    10381 tccaggagct gatgcagacc aacatggcga agatgatgga gtcgccgtgg tgggcgcaga
    10441 tgagcggcac cccgggccag atcgtcgcca tctggccgat caagaacgag acctccgagc
    10501 acatcgacga ccagctcaac acgctgctcg ccgacctgga gaccgagctg gtcaacagcg
    10561 gcgtcgtcag cgtggtcagc cgcgagcgcc aggcggagat ggccaacgag gtcgccgtcc
    10621 agcaaaaccg gtcgatctac gaccccgcct tcgccaacca gatctcgcgc cagctcggcg
    10681 cccgcttcta catcaccggc aaggtcggct cggtcgacga gttctttaat aaggagcggc
    10741 gcgtccagta cacgctgttc atccaggtga tcgaggtcga gacctcgctg gtccgcttcc
    10801 agtacaagtc cgagcgcagc aaggcgatcg ttcgctaacc tgtccttcgg agcaggtcca
    10861 tgtcgagccc gatccaacga actccgcgcc cgcggcggcg gggcctcctg ctcgccgtcg
    10921 cgggcctgat ggcctgcgcg acctactcgg agaagaccca gctcgcccgc gacgccgccc
    10981 agaccggcaa ccttcaggcc agcgagaagg tgctcaacaa gttcctgggc gtgcgccgat
    11041 ccgccgacct tccgaccgag tggaagaaag agacgatcgt cgccctgctc gagcgggcga
    11101 tggtcctgca cggcaagggc gactggaagc tcagcgcccg cgacctcgtc gccgccgaca
    11161 aacagctggt gctgctcgac gtcgcccgcg acgccggcca ggtcggcaag tgggtcttta
    11221 gcgactcgtc gggcaagtac cacgcgccgc cgtcggagaa gctggcgctc aacgccttca
    11281 acatgctcaa ctacctcctc gcggacgacc tctccggcgc ccgcgtcgag gccaagcgct
    11341 tcaccgtcac ccgcgagtac ctcggccagc tcgatccgct tgatcaggcc gcccacggcg
    11401 cgttcggcag ctacgtcgcc ggcttcgtgt tcgagcgcct cggcgagacc gagaccgccc
    11461 tgcgttacta cgacgaggcg ctgcaggagc agcccttccc gagcctgcgc gccccggtcg
    11521 cccgcctggc cgcgcgcagc agctaccgca ccccgcggat ccgcgcgctg ctcggcgagc
    11581 ccgaggcgag cgcctcacgg ccgacgaata cactgctcgc gatgcaggcg cccgagcccg
    11641 aagctcccgc ggccgcaccc gccgcgccgc cgcccgcggg tgagctgctc gtcgtgatga
    11701 acgtcggccg cgtgccttac aaggtgccgg agcggatgcc gatcggcctc gcggtcggcc
    11761 tcggcgcgac ctacgtcacc ggcgacacct c
//
LOCUS       DASWNJ010000835         5251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_327032, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000835 DASWNJ010000000
VERSION     DASWNJ010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327032"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VGB85_14225"
     CDS             <1..366
                     /locus_tag="VGB85_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135240.1"
                     /translation="ARQQQQAVRLPPRRAQAFTAAPRAPSQDPRAWAPRAPSAAPRAL
                     PPTPTFAPRNERDDRDERDEDTIIVSHPAIRSSDDRPRRRSEALPAAAPSGEPPTDGK
                     KAARPRTLPRGKHKVERVD"
     gene            691..2424
                     /locus_tag="VGB85_14230"
     CDS             691..2424
                     /locus_tag="VGB85_14230"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY0135241.1"
                     /translation="MRKLVVSSMLALAMVSWSGCKEADPNAFETHIEGIRDLNSRAAS
                     ISQLEALVKTVTTAPGDNSERINEFVGKIVPVFDEVWDQVTPEQQVTMLNMLRDVGRV
                     EGSKLWIKSLALDGSDGARKRVLAALAGIQRARATETTDAVVALLQSLVADPSKDRSK
                     QNEGEIRLELAKTLGELRDKKGVPVLIDVMKQTKENQPVSVHREAAKALGMIKDPAAV
                     DALLTVSFRVPDAPSTTDISNRAKIALVSIGEPAVPRLKEMLAGKFEEVNKLAGEHDV
                     PVLVVQQTAAGILGAMGTRSATDELVAFMPQKDCVAPDPKKKPEKKKKKKDEDEEPSE
                     EEVDDASLRAFVANSLGLIGDPRGVDAMCKCINATHNPGDMFPITEALGRIGGDAAFK
                     CLADVTRTGEYDTEALENSDFKFQIRWEAARFAILAATAEQLPALKEALTGARKENDK
                     KVGEELPKWDAGLKLLETCQSDKACYMKTLQDQNADWFAREKAAVEVARLSPGDKSVA
                     LELSRAFKVRNPDARVTMALLVPRVLGDAKCPECAAALEDVMKGEKGSMDASMQLPVL
                     IARTTIARVAE"
     gene            2536..3735
                     /locus_tag="VGB85_14235"
     CDS             2536..3735
                     /locus_tag="VGB85_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135242.1"
                     /translation="MARRLICAALLLTACAGKPATATVTPGEPGVVTTSASSPAAAPA
                     GDLSAKPTLAEAEAALAAGEPARAVALFAGYLAGEHDPAGARQAYPGLARAHEQLGDC
                     GAAIRAYDEYLARFADGAPGDLYAARGACHAELEQWQASADDFAAAFARATLPSAQVE
                     ALTRQGYAYFQLGEFEKADPPLKQADEIFLAAQEARRERFTTYYFVGMARFYRAAIHH
                     RHFRDIAIRLPEKVMAEDFARKFALLEQAQDGYNYTVKAKHMFWVSAAGYQLGALFEE
                     FYDAMMHAPVPEWLDEAQRHAYYEELKNQLRPVVNKAIWVFEKNLETARKLGYDSEFV
                     EKTEAKLSELQAVLLAGDTTLGRPHPRLVPEARPELAAGQTGAEADGAEASPVDRKLF
                     VPEPTPL"
     gene            3913..3984
                     /locus_tag="VGB85_14240"
     tRNA            3913..3984
                     /locus_tag="VGB85_14240"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3945..3947,aa:Thr,seq:tgt)
     gene            4039..4122
                     /locus_tag="VGB85_14245"
     tRNA            4039..4122
                     /locus_tag="VGB85_14245"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4074..4076,aa:Tyr,seq:gta)
     gene            4140..4212
                     /locus_tag="VGB85_14250"
     tRNA            4140..4212
                     /locus_tag="VGB85_14250"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4173..4175,aa:Gly,seq:tcc)
     gene            4241..4313
                     /locus_tag="VGB85_14255"
     tRNA            4241..4313
                     /locus_tag="VGB85_14255"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4274..4276,aa:Thr,seq:ggt)
     gene            4370..>5251
                     /locus_tag="VGB85_14260"
     CDS             4370..>5251
                     /locus_tag="VGB85_14260"
                     /inference="COORDINATES: protein motif:HMM:NF000766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEY0135243.1"
                     /translation="MAKETFVRNKPHVNIGTIGHVDHGKTTLTAAITRVLSLKGWAEF
                     KGYDQIDKAPEERARGITISTAHVEYESEKRHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVVSAPDGPMPQTREHILLGRQVGIPAMVVFLNKIDMLPEGNDGAEMIELVEVELRD
                     LLSKYQFDGDNAPIVQGSALKALNAPSVDHPDAQPIFKLMEACDSYIPEPVRELDKPF
                     LMPVEDVFTISGRGTVSTGRIERGRVKVGEEVEIVGIKDTVKTTITGVEMFRKLLDQG
                     QAGDNVGLLLRGVKREDV"
BASE COUNT          922 a         1964 c         1627 g          738 t
ORIGIN      
        1 gcccgccagc agcagcaggc cgtccgcctc ccgccgcgcc gcgcccaggc cttcaccgcc
       61 gccccgcgcg cgccctcgca agacccgcgc gcctgggccc cgcgggcgcc ctcggccgct
      121 ccgcgggccc tgcctccgac ccccaccttc gcccctcgca acgagcgcga cgaccgggac
      181 gagcgcgacg aggacacgat catcgtctcg cacccggcca tccgcagcag cgacgaccgc
      241 ccccgccggc gcagcgaggc cctcccggcc gccgccccgt ccggcgagcc cccgaccgac
      301 ggcaagaagg ccgcccgccc gcgcaccctc ccgcgcggca aacacaaggt cgagcgggtc
      361 gactagccac ttcacggagg ccactccacg gccccgtccg gggcctgggt cggcctgcgg
      421 ccgatcgggc cagaaggccc ttcgcctccg aacctcgctg accactttcg gcttcgccgg
      481 ccggtctgcg gccgatcgaa cctgaaaacc cttcatcttc gaacctgggt cggcccgttt
      541 tgggccggcc ctttcgtcgt ttggtccctg gcggacccgc ctgctcgtcc gcgatctccc
      601 gcgccgggcc gggcgacaat ttggtcgccg cgcaggactg aggcaaacaa gcgcgtgagg
      661 ctgatgtacc atcctcacaa gagaggtttc atgcgcaagc tcgtcgtttc gtccatgttg
      721 gccctggcga tggtgtcctg gtctggttgt aaagaggccg accccaacgc cttcgagacg
      781 cacatcgagg gcatccgtga tctcaacagc cgcgcggcca gcatctcgca gctcgaggcg
      841 ctggtgaaga cggtgaccac cgcgcccggc gacaacagcg agcggatcaa cgagttcgtc
      901 ggcaagatcg tcccggtctt cgacgaggtg tgggaccagg tcacgcccga gcagcaggtg
      961 accatgctca acatgctgcg cgacgtcggc cgcgtcgagg gcagcaagct gtggatcaag
     1021 tcgctcgcgc tcgacggctc ggacggcgcc cgcaagcgcg tgctcgcggc cctcgccggc
     1081 atccagcgcg cccgcgccac cgagaccacc gacgccgtcg tcgcgctgct gcagagcctc
     1141 gtcgccgatc ccagcaagga tcgcagcaag cagaacgagg gtgagatccg cctcgagctc
     1201 gccaagactc tcggcgagct gcgcgacaag aagggcgtgc ccgtgctcat cgatgtgatg
     1261 aagcagacca aggagaacca gcccgtctcc gtccatcgcg aggccgccaa ggccctcggc
     1321 atgatcaagg accccgccgc cgtcgacgcc ctgctgaccg tcagcttccg cgtccccgac
     1381 gcgccctcga ccaccgacat cagcaaccgc gccaagatcg ccctcgtctc gatcggcgag
     1441 ccggcggttc cgcggctcaa ggagatgctc gcgggcaagt tcgaggaggt caacaagctc
     1501 gccggcgagc acgacgtccc ggtgctcgtc gtccagcaga cggccgccgg catcctcggc
     1561 gccatgggca ccaggagcgc gaccgacgag ctcgtcgcct tcatgccgca gaaggactgc
     1621 gtcgcgccgg acccgaagaa gaagcccgag aagaagaaaa agaagaagga cgaggacgag
     1681 gagccgagcg aggaggaggt cgacgacgcc agcctgcggg ccttcgtcgc caattcgctc
     1741 ggcctgatcg gcgaccccag gggcgtcgac gcgatgtgca agtgcatcaa cgccacgcac
     1801 aaccccggcg acatgttccc gatcaccgag gccctcggcc ggatcggcgg cgacgcggcc
     1861 ttcaagtgcc tcgccgacgt cacgaggacc ggcgagtacg acaccgaggc gctcgagaac
     1921 tcggacttca agttccagat ccgctgggaa gccgcccgct tcgccatcct cgccgccacc
     1981 gccgagcagc tgcccgccct caaggaggcc ctcaccggcg cccgcaagga gaacgacaag
     2041 aaggtcggcg aggagctccc caagtgggac gccggcctca agctgctcga gacctgtcag
     2101 tccgacaagg cctgctacat gaagaccctg caggaccaga acgccgactg gttcgcgcgt
     2161 gagaaggccg ccgtcgaggt cgcccgcctc tcgcccggcg acaagagcgt cgcgctcgag
     2221 ctctcgcgcg cgttcaaggt ccgcaacccc gatgcccgcg tcaccatggc gctgctcgtc
     2281 ccgcgcgtgc tcggcgacgc caagtgcccc gagtgcgccg ccgccctcga ggacgtcatg
     2341 aagggcgaga agggctcgat ggacgcctcg atgcagctcc ccgtcctgat cgcccgcacc
     2401 acgatcgccc gcgtcgccga gtgatccgcg gatcgctcgg gcccgctcca ggccctgcca
     2461 aaggccgtcc acgcgctccg cgtgggcggc cttcgtcgtc ggacacgcgc cgtctggatg
     2521 tgtcatcctc cggccatggc tcgccgcctg atctgcgccg ccctgctgct gaccgcgtgc
     2581 gccggaaagc ccgcgaccgc gaccgtcacc ccgggcgagc ccggcgtcgt cacgacctcg
     2641 gcctccagcc ccgctgccgc gcccgcggga gatctctccg cgaaaccaac cctcgccgag
     2701 gccgaggccg ccctcgccgc cggtgagccg gcccgcgccg tcgccctgtt cgccggctac
     2761 ctcgccggcg agcacgaccc cgccggcgcc cgccaggcct accccggcct cgcccgcgcc
     2821 cacgagcagc tcggcgactg cggcgctgcg atccgggcct acgacgagta cctcgcccgc
     2881 ttcgccgacg gcgccccggg agatctctat gccgcacgcg gcgcctgtca cgccgagctc
     2941 gagcagtggc aagcctccgc cgacgacttc gccgcggcct tcgcccgtgc gaccctcccg
     3001 agtgcccagg tcgaggccct gactcgccag ggctacgcct acttccagct cggcgagttc
     3061 gagaaggccg accctccgct caagcaagca gacgagatct tcctcgccgc ccaggaagcg
     3121 cggcgtgagc gttttaccac gtactacttc gtcggcatgg cccgcttcta ccgcgccgcc
     3181 attcaccacc gacacttccg ggacatcgcc atccgcctcc cggagaaggt catggccgag
     3241 gacttcgccc gcaagttcgc cctgctggag caggcccagg acggctacaa ctacacggtc
     3301 aaggccaaac acatgttctg ggtctcggcc gccggctacc agctgggcgc cctcttcgag
     3361 gagttctacg acgccatgat gcacgccccc gtgcccgaat ggctcgacga ggcccagcgc
     3421 cacgcctact acgaggagct caagaaccag ctgcgcccgg tcgtcaacaa ggcgatctgg
     3481 gtgttcgaga agaacctcga gaccgcccga aagctcggct acgacagcga attcgtcgaa
     3541 aagaccgagg ccaagctgtc cgagctgcag gccgtcctgc tcgccggcga caccaccctc
     3601 ggccgcccgc acccccgcct ggtccccgag gcccgccccg agctcgccgc cggccaaacc
     3661 ggcgccgagg ccgacggcgc cgaggccagc ccggtcgacc gcaagctctt cgtccccgag
     3721 ccgaccccgc tgtaaaagac gtgctgatga tcacgcgcgg accccggcgc agccagagat
     3781 ccaggtgcgc cgaagttcgg gcgcggcttg acactccccg cgatcggacg ctatacaccg
     3841 cctccgcttt tgggccctcc cgggttcaga cgcgggccat cttcaattcg gaggaatgaa
     3901 gcgctctccc aagctggcgt agctcaatgg tagagcacct gatttgtaat caggcggctg
     3961 cgggttcaag tcccatcgcc agctctctcg cgccagtcct tctctcgctt ttcatcaacc
     4021 cctcaccaca tatctcccgg atgggtgccc gagttggcca aagggagcgg actgtaaatc
     4081 cgccgcatca ctgcttcgaa ggttcgaatc cttccccatc cacttcgtac cgactccgcg
     4141 cgggagtagc tcagttggta gagcgtcagc cttccaagct gaatgccgcg agttcgaccc
     4201 tcgtctcccg ctctcgcttc tcgaaaactc caagcatttc gcccatgtag ctcagttggt
     4261 agagcacatc cttggtaagg atgaggtcag cggttcgaat ccgctcatgg gctccctcga
     4321 aaaacctccg gcccccacac cccgggccgt cttctggaga accactgcca tggcaaaaga
     4381 aacattcgtc cgtaacaagc cccacgtgaa catcggaacg atcgggcacg tggaccatgg
     4441 aaagaccacg ctgacggccg cgatcacccg cgtgctgagc ctcaagggct gggcggagtt
     4501 caagggttac gaccagatcg acaaggctcc ggaggagcgc gcgcgcggga tcacgatctc
     4561 gacggcgcac gtcgagtacg agtcggagaa gcgccactac gcgcacgtcg actgccccgg
     4621 gcacgccgac tacatcaaga acatgatcac cggcgccgcg cagatggacg gggcgatcct
     4681 cgtcgtgtcc gcgccggacg gcccgatgcc gcagacgcgc gagcacatcc tgctcgggcg
     4741 ccaggtcggc atcccggcga tggtggtgtt cctcaacaag atcgacatgc tccccgaagg
     4801 caacgacggc gcggagatga tcgagctggt cgaggtcgag ctgcgcgacc tcctgagcaa
     4861 gtaccagttc gacggcgaca acgccccgat cgtccagggt tcggccctca aggccctcaa
     4921 cgccccgagc gtcgaccacc cggacgccca gccgatcttc aagctcatgg aagcttgcga
     4981 cagctacatc ccggagccgg tgcgcgagct cgacaagccg ttcctgatgc cggtcgagga
     5041 cgtgttcacg atctcgggtc gtggcacggt gtcgacgggc cgcatcgagc gcggtcgggt
     5101 caaggtcggt gaggaagtcg agatcgtggg catcaaggac acggtcaaga cgacgatcac
     5161 cggcgtggag atgttccgca agctgctgga ccagggccag gcaggcgaca acgtcggcct
     5221 gctgctgcgc ggcgtgaagc gcgaggacgt g
//
LOCUS       DASWNJ010000836         1701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_440913, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000836 DASWNJ010000000
VERSION     DASWNJ010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440913"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1338)
                     /locus_tag="VGB85_14265"
     CDS             complement(<1..1338)
                     /locus_tag="VGB85_14265"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0135244.1"
                     /translation="MRRLRYLPLPSLLVASLVSTSALAAPWVENKEFFSENQNFNSSK
                     VDAADLNGDLTIDLVFANGAGYDKGDDTSLLPQQAFLNDGTKMMDVSQSIFGDTPRTG
                     RAVKLRDIDNDGDNDIILGTTWVTQSQLYLNDGGGSFSNVTDPNLPQILASVGDVEVG
                     DVDNDGDLDIVLANWGVDEGAVSQTNGGITMLWTQMGAADFAQPGSGMFDDATINQMP
                     NLPVRWSWDLEFVDVDNDFDLDILVSAFAGEKASLFLFLNDGAGKFADATAGNVPQGR
                     FALDVEPMDLDQNGTVDLLTLHDGLSGRNRLLFNDAGKYSDVTGLLWKLDNVPSFDFM
                     GAFYDFDSNSKPDMVLGALQTAQIKYPDRLVYENGGKYQSNVAAFQEIKPSAGTYAIV
                     LADFNKDTRLDVAMAQNENAAEKKVLLASEEVPLDIVAPFITNFDKLNVMGPGL"
BASE COUNT          279 a          528 c          591 g          303 t
ORIGIN      
        1 caggcccggg cccatgacgt tgagcttgtc gaagttcgtg ataaacggcg cgacgatgtc
       61 cagcgggacc tcctcgctgg cgagcaggac cttcttctcg gcggcgttct cgttctgcgc
      121 catcgcgacg tcgaggcggg tgtccttgtt gaagtcggcg agcacgatcg cgtaggtgcc
      181 ggcggagggc ttgatctcct ggaacgcggc gacgttcgac tggtacttgc cgccgttctc
      241 gtagacgagg cggtcggggt acttgatctg cgcggtctgc agggcgccga gcaccatgtc
      301 gggcttgctg ttggagtcga agtcgtagaa ggcgcccatg aagtcgaagc tcggcacgtt
      361 gtcgagcttc cacagcaggc cggtgacgtc cgagtacttg cctgcgtcgt tgaacagcag
      421 gcggttgcga ccgctgaggc cgtcgtgcag cgtcagcagg tcgacggtgc cgttctggtc
      481 gaggtccatc ggctcgacgt cgagggcgaa gcggccctgc gggacattgc cggcggtcgc
      541 gtccgcgaac ttgcccgcgc cgtcgttgag gaacaaaaac aggctcgcct tctcgcccgc
      601 aaaggcggag accaggatgt cgaggtcgaa gtcgttgtcg acgtcgacga actcgaggtc
      661 ccacgaccac cgcaccggga ggttcggcat ctggttgatc gtggcgtcgt cgaacatccc
      721 ggagcccggc tgggcgaagt ccgcggcgcc catctgcgtc cacagcatcg tgatgccgcc
      781 gttggtctgg gagaccgcgc cctcgtcgac gccccagttc gccaggacga tgtcgaggtc
      841 gccgtcgttg tcgacgtcgc ccacctcgac gtcgccgacg ctcgccagga tctgcggcag
      901 gttggggtcc gttacgttgc tgaagctgcc gccgccgtcg ttgagataca gctgactctg
      961 ggtcacccag gtggtgccga ggatgatgtc gttgtcgccg tcgttgtcga tgtcgcgcag
     1021 cttgaccgcc cgaccggtcc gcggcgtgtc gccgaagatg ctctggctga cgtccatcat
     1081 cttcgtgccg tcgttgagga acgcctgctg cgggaggagc gaggtgtcat cgcccttgtc
     1141 gtagccagcc ccgttagcga acacgaggtc gatcgtcagg tcgccgttga ggtccgcggc
     1201 gtcgaccttg ctcgaattga agttctgatt ctcgctgaag aattccttgt tctccaccca
     1261 cggcgcggca agagcagagg tggatacgag cgacgcaacc agcagcgagg ggagggggag
     1321 gtaacgtaga cggcgcatga cgcctcaata gcacggctca cacgggccgg ggcgaaccaa
     1381 accggcgagc gcgaccccgc ggcgtcggcc gtcggcggcg tctgcacggg ccatccttcg
     1441 gatgggtgac ggacgaccac ggccgcggac gcgcgcgagc tacgggcgcc tgcgatccca
     1501 cgatgtgatt tcctccggcg gcgcgagacc ccaacatcag cccgcgggcc gcaggactga
     1561 aagatgaagc ggcaatgtcc gagcgacggg ggcgaaatgg cctgcgcacc gcgagcctgc
     1621 gcgagatgac ctgtcccaag agacgggtat gccgatgtgg gagcgacgag cagctcctcg
     1681 atctccatcg accatcgcgt c
//
LOCUS       DASWNJ010000837         2011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_192685, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000837 DASWNJ010000000
VERSION     DASWNJ010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192685"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..>2011)
                     /locus_tag="VGB85_14270"
     CDS             complement(89..>2011)
                     /locus_tag="VGB85_14270"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY0135245.1"
                     /translation="SATIAAVTANCGGMMHNDSKARMLAGCLAGTLFGPAGCYTGALG
                     HEPGGAEEHDPTAGEDSEDDEAGSSGGPSDTGEWPEQRALEAGNLPPVIADPGPQVID
                     EDDLLDLQLALRDPDDDPLRVWATGLPPGAQWDEPARRLRFRPDFIQGDRLWPVTIHA
                     DDGHRRVTREFTIEVVDTIAPPEPEIVGWDEFEQYTRLTISQTTDEYLDSPGNAGRTY
                     VAVITVPTAATPEDPAPVRIGLHGFATNTPAAVGSGREFRVAPHDPMNTYWWGYSAAL
                     VDDEPDPDPTPLGASEVPDYTQRRVLHLLGWLLETYPEADPTRTYVAGSSMGGAGALQ
                     IGLWYARHFAHASGALAQAVPRNHRPGRISQLEGLWGTSDGPLWDALDLTRLLRDSAE
                     ARDQYLFTRHGKDDSTIHFGAAVLPSPLTSRNLYDTLQEYGVGHLSVWDEGAHGPADP
                     LLGDNWWDEDFSPIHDSTTFVRSDLAFPAFTLSSADGDPGDGGGNGKQDWNVNAGFAG
                     TLEVPGDTGWNGEIAGALNRFLRWDATATVDTLDRFALPLHVHDGDGQDPPKAGYPSR
                     GDRFDGELPVVVDVTPRRLQQFQVQPGERVTWSFGQQRGEVTADETGSLRIPGLELGL
                     EWQVLEISRVDAGESM"
BASE COUNT          249 a          686 c          763 g          313 t
ORIGIN      
        1 ctttgcatgg cccaaacagt acctcgaccg acgcctccac gtcatggcct cggcgggccc
       61 atgcaacctc gcccagcgta cctcaggttc acatcgactc gccggcgtcg acccgcgaga
      121 tctcgagcac ctgccactcg agcccgagct cgagccccgg gatccgcagc gagccggtct
      181 cgtcggccgt cacctcgccg cgctgctggc caaaagacca ggtgacgcgc tcgcccggct
      241 gcacttggaa ctgttgcagg cggcgggggg tgacgtcgac gacgacgggc agctcgccgt
      301 cgaagcggtc gccgcggctc gggtagccgg ccttgggggg atcctgaccg tcgccgtcgt
      361 ggacgtgcag cggcagtgcg aagcggtcga gggtgtcaac cgtggcggtc gcgtcccagc
      421 gcaggaagcg gttgagggcg ccggcgatct cgccgttcca gccggtgtcg ccggggacct
      481 cgagcgtgcc cgcgaagccg gcgttgacgt tccagtcctg cttgccgttg ccgccgccgt
      541 cgccgggatc gccgtcggcc gaggacagcg tgaacgccgg gaacgcgagg tcgctgcgca
      601 cgaaggtggt gctgtcgtgg atcggcgaga agtcctcgtc ccaccagttg tcgccgagca
      661 gcgggtcggc cgggccgtgg gcgccctcgt cccagaccga gaggtggccg acgccgtact
      721 cttggagcgt gtcgtagagg ttgcggctgg tcagcgggct cggcaggacg gcggcgccga
      781 agtggatcgt cgagtcgtcc ttgccgtggc gggtgaacag gtactggtcg cgggcctcgg
      841 ccgagtcgcg cagcaaccgg gtcaggtcga gcgcgtccca cagcgggccg tcgctcgtgc
      901 cccacaggcc ctcgagctgg ctgatgcgcc ccggacggtg gttgcggggg accgcctggg
      961 cgagcgcgcc gctggcgtgg gcgaagtgac gcgcgtacca caagccgatc tgcagggctc
     1021 cggcgccgcc catcgagctg ccggccacgt aggtgcgggt cgggtcggcc tcggggtagg
     1081 tctcgagcag ccagccgagc aggtgcagga cacgccgctg cgtgtagtcc gggacctcgc
     1141 tcgcgccgag gggtgtcggg tcggggtcgg gctcgtcgtc gacgagcgcc gcgctgtagc
     1201 cccaccagta ggtgttcatc gggtcatgcg gcgcgacgcg gaactcccgg ccggagccga
     1261 cggccgcggg cgtattggtg gcgaagccgt ggagaccgat ccgcaccggc gcggggtcct
     1321 ccggggtcgc ggcggtcggc accgtgatca cggcgacgta ggtgcggccg gcgttgccgg
     1381 ggctgtcgag gtactcgtcg gtcgtctgcg agatcgtcag gcgcgtgtac tgctcgaact
     1441 cgtcccatcc gacgatctcc ggctcgggcg gcgcgatggt gtcgacgacc tcgatcgtga
     1501 attcgcgggt gacgcggcgg tggccgtcat cggcgtggat cgtcaccggc cacagccgat
     1561 cgccctggat gaagtcgggt cgaaagcgca ggcgtcgcgc cggctcgtcc cactgggcgc
     1621 ccggcggcag gccggtcgcc cacacgcgca gcgggtcgtc gtccgggtcg cgcagcgcga
     1681 gctggaggtc gagcaggtcg tcctcgtcga tcacctgcgg gccggggtcg gcgatcacgg
     1741 gcgggaggtt gccggcctcg agcgcgcgct gctccggcca ctcgccggtg tcgctgggcc
     1801 cgccgctgct gccggcctcg tcgtcctcgc tgtcctcgcc agcggtgggg tcgtgctcct
     1861 cggccccacc gggctcgtga ccgagggcgc cggtgtaaca gcctgcgggc ccgaatagcg
     1921 tccctgcgag gcaaccagcg agcatccttg ctttgctgtc gttgtgcatc atgcctccgc
     1981 agttcgccgt caccgcggcg atcgttgcac t
//
LOCUS       DASWNJ010000838         2003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_298668, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000838 DASWNJ010000000
VERSION     DASWNJ010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298668"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VGB85_14275"
     CDS             <1..773
                     /locus_tag="VGB85_14275"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0135246.1"
                     /translation="AVLLVHGRTWSGLPDFDLQVAGAEVSSMDALVAAGVAAYAVDLR
                     GYGATPRDATGFATPKQSAADVAAVLRFVQADAGVAGPPWLLGWSMGALVSALAVQMH
                     PGVARGLVLYGYPCRAGAPAPAAPDPETPQRAVNTASAAASDFITPGSVSRGVVDGFV
                     AAALKADPVRADWRGGAAWSELRFEALAVPVLVLQGEHDPFVDRGCMADRFAELKGVD
                     RRWQLLAGADHAAHLETAGPRFVAAVLGFIREGAAWSE"
     gene            856..1533
                     /locus_tag="VGB85_14280"
     CDS             856..1533
                     /locus_tag="VGB85_14280"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEY0135247.1"
                     /translation="MTYPEAHEDFPWGEVAIKVKNKVFLFLHSNGETLSLSCKLPQSS
                     DMALMLPFAKPTGYGLGKSGWVSASFNNKNADEEPPVELLREWIDESYRAVAPKTLVA
                     EMRGGAAGERVAGAGGAKAGKVGGGAKVGKTGGAAKVGKTGGAAKVGKTGGAAKVGKT
                     GGAAKVGRTGGAAAKAGSKVTKAAKTGSKVTKAAKSAKAGSGAKSAKATTAGARSKKT
                     AAKAGAR"
     gene            1793..>2003
                     /locus_tag="VGB85_14285"
     CDS             1793..>2003
                     /locus_tag="VGB85_14285"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0135248.1"
                     /translation="MIEIKVESPGQAPRVQRFSSTVITVGRATDCELCVDDPSISRRH
                     CRISRAADGWFLEDLGSANGTGVAGE"
BASE COUNT          307 a          582 c          842 g          272 t
ORIGIN      
        1 ccgcggtgtt gctggtgcac gggcggacct ggagcggcct gccggacttc gatcttcagg
       61 tggccggcgc ggaggtgtcg tcgatggacg cgctggtcgc cgcgggggtc gctgcctatg
      121 cggtcgacct gcgcggctac ggagccacgc cgcgcgacgc gaccggcttt gccaccccga
      181 agcagtcggc cgcggacgtc gccgcggtgc tgcgcttcgt gcaggccgac gccggggtcg
      241 cggggccgcc gtggctgctc ggctggtcga tgggcgcgct ggtctcggcg ctcgcggtgc
      301 agatgcaccc cggggtcgcg cgcgggctgg tgttgtatgg atatccgtgc cgcgcgggcg
      361 cgccggcccc ggccgcgccc gacccggaga cgccgcagcg ggcggtgaac acggcgagcg
      421 cggcggcctc ggacttcatc acgccgggct cggtgtcgcg gggggtggtc gatggtttcg
      481 tcgcggcggc gttgaaggcc gacccggtgc gcgccgactg gcgcggcggt gcggcgtgga
      541 gcgagctgcg cttcgaggcg ctggcggtgc cggtgctcgt gctgcagggc gagcacgacc
      601 ccttcgtcga tcgcgggtgc atggcggacc gcttcgcgga gctcaagggt gtcgaccgcc
      661 gctggcagct cctcgccggg gccgatcacg cggcgcacct cgagaccgcc gggccccgct
      721 tcgtcgcggc ggtcctcggc tttatccgcg agggcgcagc gtggagcgaa tgatcgggca
      781 atatcgagcg acggagaccc accgatgccg ccgaacagcc tgcaacgaac cgccactgcc
      841 ctgcgcgacg ccgcgatgac ctaccccgag gcccacgagg acttcccgtg gggcgaggtc
      901 gcgatcaaag tgaagaacaa ggtgttcctg ttcctgcaca gcaacggcga gacgctgtcg
      961 ctgtcgtgca agctgccgca gtcgagcgac atggcgctga tgctgccgtt cgccaaacca
     1021 accggctacg gcctcggcaa gagcggctgg gtcagcgcga gttttaataa taaaaatgcg
     1081 gacgaggagc cgccggtgga gctgttgcgg gagtggatcg acgagagcta tcgcgcggtc
     1141 gcgccgaaga cgctggtcgc ggagatgaga ggcggagcgg cgggggaaag agtcgcgggg
     1201 gcaggtgggg cgaaggcggg gaaggttgga ggtggcgcga aggtggggaa gaccggaggc
     1261 gctgcgaagg tggggaagac cggaggtgcg gcgaaggtgg ggaagaccgg aggcgcggcg
     1321 aaggtgggga agaccggagg cgctgcgaag gtcgggagga ccggaggcgc ggcagcgaaa
     1381 gcggggagca aggtgacgaa ggcggcgaag acggggagca aggtgacgaa ggcggcgaag
     1441 tcggcgaagg ctgggagcgg ggcgaagtcg gcgaaggcaa cgaccgcagg tgcgaggagc
     1501 aagaagacgg ccgcgaaggc cggggcccgg taggtcggcg aaggcagcga ccgcaggggt
     1561 gaggggcagc cgcggaggcc ggggcgggtc gcggagatcc gggcccggtc gggcgaggtc
     1621 gtggcgagcg agcgccggtg aggggggccc cgggccgggt ctgcggcaga tcgtgtgaag
     1681 tgcattacat aatctgccac cggttttgaa atgcgaggct cgcgtccccg gaaattgtcg
     1741 atgcgcatgc actggagcgg ccggggctcg ctgctatgct ggtctccgct tcatgatcga
     1801 gatcaaagtg gaatcaccgg ggcaggcgcc ccgggtgcag cgtttctcgt cgacggtgat
     1861 cacggtgggt cgcgcgacgg actgcgagct gtgtgtcgac gatccgtcga tctcgcggcg
     1921 ccattgccgg atctcccggg ccgcggacgg ctggtttttg gaggacctcg gctcggcgaa
     1981 cgggacgggg gtcgcgggcg agc
//
LOCUS       DASWNJ010000839         4236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_367916, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000839 DASWNJ010000000
VERSION     DASWNJ010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367916"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VGB85_14290"
     CDS             <1..710
                     /locus_tag="VGB85_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135249.1"
                     /translation="ACALGDVTGCTGAGDLLSDPEGPADAKSKALAMFVKACVGHDGY
                     GCLRVGVAFHDGIGTPQDLAKAKAHFVRACEWSNKDGCHAAEQMAAANGGEIELELTT
                     RAPTIGEGGLEAREVSCRMAEQGLPALTEVLAALARNKGALDACAKEGAAVAVSWEFA
                     GGEIKAARVNGNPAKKVAKCVGWTMRKTKLRGSGSCDAVLLLGDAEGAAKALAARDPK
                     DDGRKHVRVTSEGDVVE"
     gene            1243..2937
                     /locus_tag="VGB85_14295"
     CDS             1243..2937
                     /locus_tag="VGB85_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135250.1"
                     /translation="MKLRSLALLLVIPLLGCGLGALDALQDVPVCDEQGGTDAKCPNS
                     GMSSSGGDVTPTTSGGDDGIATSTDATSLPDLPDAGESSSGEPPAPPEIVQVKLDPAS
                     PLTQAASMKVQVEALHSAGVTMALDGGAPVSLAATGANFYKGAIEVYGASMNGGHTIM
                     VVASSGELLDTLPVDFDVDAPPSGANMWLKTSEVPDSQTRAIAVDAAKNVYEVGIAGK
                     GAATRLVVNKRDEYGEPAWPGMWTTYHDGESWGEDVAIGPDGMIHVLGNYKDKDDHTR
                     WWLARLDPAVGIVVGKPQLGEIDEPARGLSIAENGDVAIVGHATVWGAEPQMDDVQTK
                     VWLRPKAGGGVITEWGYSPSDPGPNWFTEMPEDILIAGGRVFVTGSAEGVYKDAVKQD
                     IPVKRTFVIELDHKGTVVREYVAPGLPHEQSGGYALAIDGKGGIASAGWACNDPCVKV
                     GEVQWFAGDGDLVPYARQVEAGKDGPGYSLDIAYSPAGYNVVASAVSMGAGLNLALRV
                     TGRRSDNLELVFEYTFDTNSLEMGQAVAVGPNGYLWFAGLRVIDNALRAVVGRGNG"
     gene            complement(2934..>4236)
                     /locus_tag="VGB85_14300"
     CDS             complement(2934..>4236)
                     /locus_tag="VGB85_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135251.1"
                     /translation="RAVTEHSRRKGESLERSKDAVVRASAFSSITQAFAQQTAHFTQM
                     RDFAPAEVYPSRMRASFLAVCLAPAAILACGSGPGEGFASTMGPAITTASGGTNPAGS
                     TSGVDSSTSAAAGSTSGEAGSASTSSTSAQDSAASTGTIYDLGTGGDLGPLEPPGCKD
                     KIDFLFMMQRAGQSPEEQLKLIAAFPKFIDTIQDRFTGFDVHILVTDADWTWGRHDCD
                     PQGCLVTNNNGCLDDGYLIPDYPCGMYETVMKDGCNFKLGAGLTFNAGDGAANVPCAI
                     DDDRRYITAEQSNLKETFACMAQVGIGGGGRNAAAIVAAVGPELNDDGGCNAGFLRSD
                     ALLMITMAAGTYDEISEGGPQQWADAVFAAKGNNESSVIMLGIGGWDPDPYSSKGRLS
                     QFIKRFPYHVYGHDDAPDYGAHFAAAVELVDEACKGFTPPG"
BASE COUNT          718 a         1316 c         1535 g          667 t
ORIGIN      
        1 aggcgtgcgc gcttggggat gtcaccggct gcacgggggc cggcgacctg ctgtcggatc
       61 cggaggggcc ggcggacgcg aagtcgaagg cgctggcgat gttcgtcaag gcgtgcgtcg
      121 ggcacgacgg atacggctgc ctgcgcgtcg gggtcgcgtt ccacgatggc atcggcacgc
      181 cgcaggacct ggcgaaggcg aaggcgcact ttgtccgcgc gtgcgagtgg tccaacaagg
      241 acggctgtca cgcggcggag cagatggcgg cggccaacgg cggggagatc gagctcgagc
      301 tgacgacccg ggcgccgacg atcggcgagg gcgggctcga ggcgcgcgag gtgtcgtgcc
      361 ggatggccga gcaggggctc ccggcgctca cggaggtgct cgccgcgctc gcgcgcaaca
      421 agggcgcgct cgatgcatgt gcaaaggaag gcgccgcggt cgcggtcagc tgggagttcg
      481 cgggcggcga gatcaaagcg gcgcgcgtca atggcaaccc ggcgaagaag gtcgccaagt
      541 gcgtcggctg gacgatgcgc aagaccaagc tgcgcggcag cgggagctgc gacgcggtgc
      601 tgctgctggg cgacgccgag ggcgctgcca aggccctcgc cgcgcgcgac cccaaggacg
      661 acggccgcaa gcatgtgcgc gtcaccagcg agggcgatgt cgtggagtaa cgcgggccga
      721 gcgacggccg cggacccgcg cggcccatcg ctcgcggttg ccgacggtgc tcttgaggcg
      781 caaaaggttg gtctcttcgt ccgtcagatc cagggctgag cgggcgatct ccgtactttg
      841 aaagccgtcg gtctcggtgg caaaaagtcg ccgtcctatc agcagttcgt ggaggacaac
      901 ggccaccgag aagccgtcgc tttgaggctg gaccggatag acacccggga gtcaccggcc
      961 acgcggtggg catcaatcgg tgcatctggg gtcgctcgcc cccacagatg gcgattcgcc
     1021 ccgaccccgg cgtagcccgc aggggcgagt tcgagcgtat agttggtcca tgctcgcgca
     1081 tgcctgcatc gccaccgcgg ccctcgtcgc gcccgtgccc gcgacgtcgc gcgggacagc
     1141 ggcgaggagg ccctcgaact ccatgggaag aggagccacc ccacggtcgg ctgtggccgc
     1201 gtcgggttcg tcatggaagg acgtcgtgaa atgagaagcc acatgaaact ccgctcgctg
     1261 gcccttttgc tggttatccc actgctgggc tgcggcctgg gtgcgctgga tgcattacaa
     1321 gatgtcccgg tctgcgacga acagggtggc accgacgcca agtgcccgaa tagtggcatg
     1381 agtagcagcg gcggggacgt gacgccgacg acgtccggtg gggacgacgg gatcgcgacg
     1441 tccaccgacg cgacgtcgct ccccgatctc ccggatgcgg gcgagtcgtc gtccggggag
     1501 ccacccgcgc cgccggagat cgttcaagtc aagctcgacc cggcctcgcc cctgacccag
     1561 gcggcgtcga tgaaggtgca ggtcgaggcg ctccactccg ccggggtcac gatggcgctg
     1621 gacggcggag cgcccgtgtc gctggccgcg accggggcga atttttataa gggcgcgatc
     1681 gaggtgtacg gcgcttcgat gaatggcgga cacacgatca tggtggtcgc gtccagcggc
     1741 gagttgctcg acacgctgcc cgtagacttc gatgtcgacg ctcccccgtc aggggcaaac
     1801 atgtggctca agaccagtga ggtcccggac agccagacgc gcgcgatcgc cgtcgacgcc
     1861 gcgaagaatg tctacgaggt cggcatcgcg ggcaaggggg cggcgactcg gctcgtcgtc
     1921 aacaagcgcg acgagtacgg cgagccggcg tggccgggga tgtggaccac ctaccatgac
     1981 ggcgagtcgt ggggagagga cgtggcgatc ggcccggacg ggatgatcca cgtgctcggg
     2041 aattataagg acaaggacga ccacacgcgc tggtggctgg ccaggctcga tcccgccgtc
     2101 gggatcgtcg tcgggaagcc gcaactgggc gagatcgacg agcccgcgcg cgggctgagc
     2161 atcgccgaga acggagatgt ggcgatcgtc ggacatgcca cggtctgggg ggcggagccg
     2221 cagatggatg atgttcagac caaggtgtgg ctgcgcccga aagcgggggg cggcgtgatc
     2281 acggagtggg gctacagtcc gtcggacccc ggtccgaact ggttcaccga gatgcccgag
     2341 gacatcctga tcgctggcgg tcgggtcttc gtgaccggaa gcgccgaggg tgtttacaag
     2401 gatgctgtca aacaggatat tccggtgaag cgaaccttcg tgatcgagct cgatcacaag
     2461 ggtaccgtcg tccgggaata cgtcgctccc ggtctacccc atgagcaaag cggtggttat
     2521 gcgctggcga tcgacggcaa gggtggcatc gcctccgccg ggtgggcatg caacgatccc
     2581 tgtgtgaagg tcggtgaggt ccagtggttc gcgggcgacg gagacctcgt accttacgcg
     2641 cgccaggtgg aggccggcaa ggacggcccg gggtattccc tggatatcgc gtacagcccg
     2701 gcgggatata acgtcgtcgc ctcggcggtg agcatggggg caggcctcaa cctggccttg
     2761 cgtgtcacgg ggcggcgctc cgacaacctg gagctcgtct tcgagtacac gttcgacacg
     2821 aactcgctcg agatgggcca ggccgtcgcg gtcgggccga acggctactt gtggtttgcc
     2881 ggcctgaggg tgatcgacaa cgccctgcgg gcggtcgtcg gacgaggcaa cggctagccc
     2941 gggggggtga accccttgca cgcctcgtcc acgagctcca ccgccgccgc gaagtgcgcc
     3001 ccgtagtcgg gagcgtcatc gtgaccatac acgtgatagg gaaacctctt tataaactgc
     3061 gagaggcgtc ccttcgacga atacggatcc gggtcccatc cgccgatccc gagcatgatc
     3121 acggagcttt cgttgttgcc cttcgcggcg aacacggcgt ccgcccactg ctgaggaccg
     3181 ccctcggaga tctcatcgta ggtcccggcg gccatggtga tcatcaacag ggcgtcgctt
     3241 cggaggaacc cggcgttgca tccgccgtcg tcattgagct cggggccgac cgcggcgacg
     3301 atcgcggcgg cgttacggcc gccgccgccg atgcccacct gtgccatgca cgcgaacgtc
     3361 tccttgaggt tgctctgttc tgcagtgatg tagcggcggt catcgtcgat cgcgcaaggt
     3421 acgttcgccg cgccgtcgcc cgcgttgaag gtcagcccgg cgcccagctt gaaattgcag
     3481 ccgtccttca tgaccgtctc gtacatcccg caggggtaat ccgggatcaa gtaaccgtcg
     3541 tccaggcagc cgttgttgtt ggtgaccaaa cagccttgcg ggtcgcaatc gtgccgcccc
     3601 caggtccaat ccgcgtcggt gaccaggatg tgcacgtcga agccggtgaa cctgtcctgg
     3661 atcgtgtcga tgaacttcgg gaacgcggcg atcaacttca actgctcctc cggcgactgc
     3721 ccggcgcgct gcatcatgaa caaaaagtcg atcttgtcct tgcagccggg gggctccagc
     3781 gggccgaggt cgccgcctgt gccgaggtcg tagatcgtgc cggtgctggc ggcgctgtcc
     3841 tgcgcggagg tcgagctggt ggaggccgag ccggcctcgc ccgaggtcga gccggccgcc
     3901 gcggaggtcg agctgtccac acccgaggtc gagccggccg ggttcgtgcc gcccgacgcg
     3961 gtggtgatcg ccggccccat ggtggacgcg aagccctcgc cagggccact gccacacgcc
     4021 aggatagccg cgggagcgag acagaccgcc aggaaggatg cgcgcatccg tgaaggatag
     4081 acctcggcag gggcgaagtc acgcatttgc gtgaagtgcg ccgtctgctg ggcgaaagct
     4141 tgggtaatag aggagaaagc agaagccctg accacggcgt ctttggaccg ctccaagctc
     4201 tcacctttgc ggcgtgaatg ctcggtgaca gcgcgg
//
LOCUS       DASWNJ010000840         2899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_227196, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000840 DASWNJ010000000
VERSION     DASWNJ010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227196"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="VGB85_14305"
     CDS             <1..1015
                     /locus_tag="VGB85_14305"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0135252.1"
                     /translation="DAPERFRGVTRDAVYDLASLTKVLCTGTLAAIAVSEGRLALASE
                     VPAPWSAACPDATLADLLRHSAGLAAHREYFAELGRRGPAARVLERVCATPPAYIAGT
                     QTIYSDLGFMILGAWIERVFDAPLDRVFADRVAWPLGLDAGTVPRLAFHRLQGARGGT
                     TAEQALVVPTEVYDPALHPSGPPTWFACREPARVAHYEVHDDNAFVMNGVAGHAGLFG
                     DAEAVLEVARGWLLGGIPGLEDRWRRTFLEHRSPVPGSTRRLAWDGPNPDGSGSTGAA
                     LSSAAAGHLGYTGTSLWIDPSPPDGRGPVIAVLLSNRVHPTRDNPAIVQLRPRFHRAA
                     ASL"
     gene            complement(1163..2236)
                     /gene="recA"
                     /locus_tag="VGB85_14310"
     CDS             complement(1163..2236)
                     /gene="recA"
                     /locus_tag="VGB85_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEY0135253.1"
                     /translation="MAVLRSSKNTDNNNGTGEVRSIIGKAIDAIVQRFGKGAIMALGG
                     DPGERPSFEVVSTGSLNLDKALGIGGLPRGRIIEIYGPESSGKTTLTLHTIAEAQKAG
                     LVCAFIDAEHALDVKYAARLGVRLDEVLISQPDCGEQALEIVDTLARTGGVGLIVVDS
                     VAALIPRAELEGDMGDQHLGLQARLMSQAMRKISGVAHTTGTMIVFINQLRHKIGVTF
                     GSPETTTGGNALKYFASVRLDIRRIATVKDGEDAVATRARVKVVKNKCAPPFTEAQFE
                     ISFGTGINRDAEILDWALERGVIVKSGSWFSLGDIRIGQGRANSLEWLTQHPVERDAL
                     LARAMVDLGATGAAVPGIEAMAA"
BASE COUNT          378 a         1151 c          902 g          468 t
ORIGIN      
        1 cgacgccccc gagcgctttc gcggcgtcac ccgcgacgcc gtctacgacc tcgcctcgct
       61 gaccaaggtc ctgtgtaccg gcacgctcgc cgcgatcgcc gtgtccgagg gtcggctcgc
      121 gctcgccagc gaggtccccg ccccgtggtc cgccgcctgc cccgacgcga ccctcgccga
      181 cctgctgcgc cacagcgccg ggctcgccgc gcaccgcgag tacttcgccg agctcggccg
      241 ccgcggcccg gccgcgcgcg tactggagcg cgtgtgtgcc accccgccgg cctacatcgc
      301 cggcacgcag acgatctaca gcgacctcgg ctttatgatc ctcggcgcct ggatcgagcg
      361 ggtgttcgac gctcccctcg accgcgtgtt cgccgaccgc gtcgcctggc cgctcggcct
      421 cgacgccggc accgtccccc gcctcgcctt ccaccgcctc cagggcgccc gtggtggcac
      481 caccgccgag caggccctcg tcgtccccac cgaggtctac gaccccgccc ttcatccctc
      541 gggcccgccg acctggttcg cctgccgcga gccggccagg gtcgcccact acgaggtcca
      601 cgacgacaac gccttcgtca tgaacggcgt cgccggccac gccggcctgt tcggcgacgc
      661 cgaggccgtg ctcgaggtcg ccaggggttg gttgctcggc ggcatccctg gcctcgagga
      721 caggtggcgc aggaccttcc tcgagcaccg ctccccggtc cccggctcga cgcgccggct
      781 cgcctgggac ggcccgaacc cggacggcag cggctcgacg ggcgcggccc tctcgtccgc
      841 cgccgccggc cacctcggct acaccggcac cagcctgtgg atcgacccga gcccgccgga
      901 cggccgcggc ccggtgatcg ccgtgctgct gagcaaccgc gtccacccga cccgcgacaa
      961 cccggcgatc gtgcagctcc gccccaggtt ccaccgcgcc gccgcctctc tgtagcctgt
     1021 cacggcggcg ccgcaacgct gcggcgccat cactgcagtg ctttatcgcc gtgacaccct
     1081 tgtggcgcta ctcgctgcgg cgccgcatcg ctgcggcgcc gcatcgctgc ggcgcctgca
     1141 cgagcagcgc ccgcggccct cgtcaggccg ccatcgcctc gatgccgggc accgccgccc
     1201 cggtcgcgcc gaggtcgacc atcgcccgcg cgagcagagc gtcgcgctcg accgggtgct
     1261 gcgtcagcca ctcgagcgag ttggcccgcc cctgcccgat ccggatgtcg ccgagcgaga
     1321 accagctgcc cgacttgacg atcacgccgc gctcgagcgc ccaatcgagg atctccgcgt
     1381 cgcggttgat gccggtgccg aacgagatct cgaactgcgc ctcggtgaac ggcggcgcgc
     1441 acttgttctt gaccaccttc acccgcgccc gcgtcgccac cgcgtcctcg ccgtccttga
     1501 ccgtggcaat tcgccgaatg tcgagccgca cgctcgcaaa atacttcagc gcgttgccgc
     1561 cggtggtcgt ctccggcgac ccgaacgtca ccccgatctt gtgccgcaat tgattgatga
     1621 acacgatcat cgtccccgtg gtgtgggcga cccccgatat cttgcgcatc gcctggctca
     1681 tcagccgcgc ctgcaggccg aggtgctggt cacccatgtc gccctcgagc tccgccctcg
     1741 ggatcagcgc cgcgaccgag tcgacgacga tgagcccgac cccgccggtc cgcgccagcg
     1801 tatcgacgat ctcgagcgcc tgctccccgc agtccggctg cgagatcagc acctcgtcga
     1861 gcctgacccc cagcctcgcc gcatacttca cgtcgagcgc gtgctcggcg tcgatgaacg
     1921 cgcacacgag cccggccttc tgcgcctcgg cgatcgtatg cagcgtcagc gtcgtctttc
     1981 cagaggactc cggcccgtag atctcgatga tgcgcccccg cggcagaccg ccgatgccga
     2041 gcgccttgtc gagattgagc gagccggtcg acaccacctc gaagctgggc cgctcgccag
     2101 ggtcgccgcc cagcgccatg atcgcgccct tgccgaagcg ctggacaatt gcgtcgatcg
     2161 ccttgccgat gatcgaccgg acctcgccgg tgccattgtt gttgtcggta ttcttggatg
     2221 aacgaaggac tgccatgctc ccctctttgc tcgtcgcgtg cccggcccgc ccatgtgtcc
     2281 tttcgggcac ttggccgtct cgctgccctc tcgccggcca acctgcggga cacccgtccc
     2341 gcccgttggc cgctcgctct tgatggcgtg ctcgagacag caagggtcct cggactcgag
     2401 gtggcacgct cgagatagcc aggtccgcgg actcttgatg gcgcgctcga gatagccagg
     2461 tccgcggacc cttgatggcg cgctcgctaa acgcggggcg ggggatacct gggccgggga
     2521 gcgggggccg ccggacggcg ggtccctgcg acgcgggtcc tcccagggga cgtgctcgcc
     2581 gccccatccg tcacgattac cggatcgctc acgccgagcc ttattcccgt gaccgaccgt
     2641 tgacggggcg tctgcgcgcg acccctgggt cctcccgctg cttcccccgc tcgtcggccc
     2701 ccggtccccg ggccaacatt gtctgcgccc cgcttttcgc taaacgcgaa tcgccgctcg
     2761 agcgaaaaac gcatcgcggc gagttcgagt gccgacgggg ctctgctccg tgggtggtta
     2821 atttggtatc tcctaaaggt gccgtatacc cgcgcaaacg ctgttctgat cacaatgcag
     2881 atgctcgaca acggtcacc
//
LOCUS       DASWNJ010000841         4052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_120983, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000841 DASWNJ010000000
VERSION     DASWNJ010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120983"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="VGB85_14315"
     CDS             <1..1154
                     /locus_tag="VGB85_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135254.1"
                     /translation="SINTLVGGAGKTGGAARDWVNSTGQTVPKLTTDQSMAIFTYVYP
                     TYVGYTKDIIRKWGGNWDAYPQKMKEVLVDLRFRGDLSDRHKAHLLPSIKAADYNAFR
                     AAIQNHKYWQDHTNLKNDKKGNINQRITARSAWLTGEAVVAAPAATDWSFPFQNPDGK
                     EIHDAGHYLGSPTLATAALPKALDGYYPIGASGIWHGGIHFDTGSGTQLRQRDGVRCI
                     KDGEVVAYLVNKTYAEIEFKPSPNKALYSTGFTLVRHRLECPAPPPPPPPPAPPAKTA
                     PAKTAPAKPGPPVKPGPPAKTAPAKPAPAPAPTSNVLTFYSLYMHQLDYQGYTEDPKR
                     ARPAYWGEPREFVVGEKAKDTQDDAEVLALAAFVEGEPDLLDDCSEDAC"
     gene            1182..3392
                     /locus_tag="VGB85_14320"
     CDS             1182..3392
                     /locus_tag="VGB85_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135255.1"
                     /translation="MTNDKVIGLNIRRDPNGKSPRLGLLPRGAKIAIGARSPDGKWGQ
                     IKQVISGTIAPVQQGGVVAPTAATGWVFLGELDPGPAEPTTFDDVVVPPKPVPIRAGD
                     LIGYLGEYQQHVDALPTPVRGRRPMLHLEILTGESVPAFVNKCRAYARTLPAGSGSLF
                     VIDKGAKLVVQTNPSGEIAGTDQAVPVGDATGAWVKVRRATKTIAEKATLGAYNSANH
                     TYASGAVWTGWYVGPTNNLRTQSDAAAKKAGYTRREVMVPVGPPVWVEKAALDARGAT
                     ATPLKTWNAFPLHLANAKDPAVGLTRVMTRTELERTPQAQRATDPEGNHWWRVSARTA
                     AIDDKGHTAVVGWACEKGHDKVSWQSPWAWPGFEYVEEGAIETVDMWSTALLRTGMAK
                     ETEFTDFTARADKVDKSALLQKLYELIDTNKNGIFEPNELHAAMQQPLLSQALARVVT
                     RYESEWGGDDSKWVALDPLVLDGIPEWQAEKQRIKSLRWWPKVAGKVQGFPASPVAYH
                     IHPIGLIANFHGEPAAAPAESAPAARRTANGPPEKSGRHWVSRFMSSSSISDLKQPFQ
                     GNVAKFFAALKAGGVSYNINTTLRPPQRSYLMYYAREVASGRLAPDKVPPFVPQENDE
                     PVNIDWAHKDSAGKPDLVAAKKAARDMDSAYGAAGAIGKPYRSNHNGGRAIDVRFTPA
                     WGIGKSVVDANGKTVKIGSKRDILNIGGTYNVFHWNYHGAKAKEDDPHWSATGN"
     gene            complement(3398..3922)
                     /locus_tag="VGB85_14325"
     CDS             complement(3398..3922)
                     /locus_tag="VGB85_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135256.1"
                     /translation="MFKIPRQQMAEIARTAARNYEDRMVRQLGKQFPGGCFGVSRDDL
                     ADMVQQGIERAAMRKITSEKHVAKFIALATALGQEFDMDPKLPWAGELLDQITPENVP
                     EVLDALSHKAAEHLMTSAGADNVGSANASEAAGALTGPPQPGDEPLSPAADVGDPVAP
                     CPKLARRRLYSFSS"
BASE COUNT          729 a         1423 c         1360 g          540 t
ORIGIN      
        1 cgagcatcaa tacgctcgtg ggcggcgccg gcaagaccgg cggggccgcg cgcgactggg
       61 tgaacagcac cggccagacc gtccccaagc tgaccaccga tcagtcgatg gcgatcttca
      121 cctacgtgta cccgacctac gtcggatata cgaaggacat catcaggaag tggggcggca
      181 actgggacgc gtatccccag aagatgaagg aggtcctggt cgacctgagg tttcgcggtg
      241 acctgtccga ccggcacaag gcgcacctgc tgccgtcgat caaggccgcc gactacaacg
      301 cctttcgcgc ggcgatccag aaccataaat actggcagga ccacaccaac ctcaagaacg
      361 acaagaaggg caacatcaac cagcggatca ccgcccgcag cgcgtggctg acgggcgagg
      421 cggtggtggc cgcccccgcc gcgaccgact ggtcgttccc gtttcagaac cccgacggca
      481 aggagatcca cgacgccggg cactatctcg gctcgccgac gctggcgacc gccgcgctgc
      541 cgaaggcgct cgatggttat tacccgatcg gcgcgagcgg catctggcac ggcgggatcc
      601 acttcgacac cggcagcggc acccagctgc ggcagcgcga cggcgtgcgc tgcatcaagg
      661 acggcgaggt cgtcgcgtat ctcgtcaaca agacctacgc ggagatcgag ttcaagccga
      721 gccccaacaa ggcgctgtac tcgacgggct tcacgctggt ccgccatcgc ctcgagtgcc
      781 ctgccccgcc accaccgccg ccaccgccgg ccccgcccgc caagacggcc cccgccaaga
      841 ccgcaccggc caagcccggg ccgcccgtca aacctgggcc gcccgccaag accgcgcccg
      901 ccaagccggc gcccgcgccc gcgccgactt ccaatgtgct gactttctac agcctgtaca
      961 tgcatcagct cgattatcag ggttacaccg aggaccccaa gcgggcgcgc ccggcctact
     1021 ggggggagcc gcgagagttc gtcgtcggcg agaaggccaa ggacacccag gatgacgcgg
     1081 aggtgctcgc cctggcggcg ttcgtggagg gcgagcccga cctgctcgac gactgctccg
     1141 aggatgcctg ctgatcgcca cggcgacgta cgaggattcg catgaccaac gataaagtca
     1201 tcggattgaa catccgccgc gacccgaacg gcaagagccc ccggctcggc ttgctgccgc
     1261 gtggggcgaa gatcgcgatc ggggcgcgct caccggacgg caaatggggg cagatcaagc
     1321 aggtgatctc ggggacgatc gcccccgtgc agcagggcgg cgtggtcgcg ccgacggcag
     1381 cgaccggctg ggtgttcctc ggtgagctcg acccgggccc ggccgagccg acgaccttcg
     1441 acgacgtcgt cgtgccgccg aagccggtgc cgatccgcgc cggcgatctg atcggatatc
     1501 tcggcgaata tcagcagcac gtcgatgcct tgccgacgcc ggtgcgtggc cggcggccga
     1561 tgctccacct cgagatcctc accggcgaga gcgtcccggc gttcgtcaac aagtgccgcg
     1621 cgtacgccag gacgctgccc gccggcagcg gctcgctgtt cgtgatcgac aagggcgcga
     1681 agctggtcgt gcagaccaat cccagcggcg agatcgccgg caccgaccag gccgtgccgg
     1741 tcggggacgc gacaggcgcg tgggtcaagg tccgccgcgc gacgaagacg atcgccgaga
     1801 aggcgaccct cggcgcgtac aacagcgcca accacaccta cgccagcggc gcggtgtgga
     1861 ccggctggta cgtcgggccg accaacaatc tccgcaccca gagcgacgcg gcggcgaaaa
     1921 aggccggcta cacgcgccgc gaggtgatgg taccggtcgg cccgccggtg tgggtcgaga
     1981 aggcggcgct cgacgcccgg ggcgccaccg cgacgccgct caagacgtgg aacgcgttcc
     2041 ccctgcacct cgccaacgcc aaggatcctg cggtcgggct gacgcgggtg atgacgcgga
     2101 cggagctcga gcgcacgccg caggcccagc gcgcgaccga cccggagggc aatcactggt
     2161 ggagggtctc ggcgcgcacg gccgcgatcg acgacaaggg tcacaccgcc gtcgtcggct
     2221 gggcctgcga gaagggccac gacaaggtgt cctggcaatc tccgtgggcc tggccgggct
     2281 tcgaatacgt cgaggagggc gcgatcgaga cggtcgacat gtggtcgacg gcgctgctgc
     2341 gcacgggcat ggccaaggag acggagttca ccgacttcac ggcccgcgcg gacaaggtcg
     2401 acaagagcgc gctgctccaa aagctctacg agctgatcga caccaacaag aacggcatct
     2461 tcgagccgaa cgagctgcac gcggcgatgc agcagccgct gctgtcgcag gcgctggccc
     2521 gcgtcgtgac gcgctacgag agcgagtggg gcggcgacga ttcgaagtgg gtcgcgctcg
     2581 atccgctggt gctcgatggc atcccggagt ggcaggccga gaagcagcgg atcaagagcc
     2641 tgcgctggtg gcccaaggtc gccggcaagg tccagggctt ccccgcgagc ccggtcgcct
     2701 atcacatcca cccgatcggc ctgatcgcga acttccacgg tgagcccgcc gcggcccccg
     2761 ccgagtcggc ccccgccgcg cgcaggaccg cgaacggacc gccggagaag agcggcaggc
     2821 actgggtcag ccgattcatg tcgagcagct cgatctccga cctcaagcag cccttccagg
     2881 gcaacgtcgc caagttcttc gcggcgctca aggccggcgg cgtcagctac aatatcaaca
     2941 ccacgctgcg cccgccccag cgatcatacc tgatgtatta cgcgcgcgag gtggcctccg
     3001 gtcgcctggc gcccgacaag gtgcccccgt tcgtgccgca ggagaacgac gagccggtca
     3061 acatcgactg ggcccacaag gacagcgccg ggaagcccga cctggtcgcc gccaagaagg
     3121 ccgcccgcga catggacagc gcctacggtg cggcgggggc gatcggcaag ccctacaggt
     3181 cgaaccacaa tggtggcagg gcgatcgacg tcaggttcac gccggcgtgg ggcatcggta
     3241 agagcgtcgt cgatgccaac ggcaagaccg tcaagatcgg ctcgaagcgc gatatcctca
     3301 acatcggcgg cacgtataac gtctttcact ggaactacca cggcgccaag gccaaggagg
     3361 acgacccgca ctggtccgcg accggcaatt gagccggtca cgacgagaac gagtacagcc
     3421 gccgccgcgc gagcttcggg cacggcgcga ccggatcgcc gacatcagcg gccgggctca
     3481 ggggctcgtc gccgggctgg ggcgggccgg tcagggcccc ggccgcctcg ctcgcattgg
     3541 cactaccgac attgtccgcg ccggcgctcg tcatcaggtg ctcggcggcc ttgtggctca
     3601 gcgcgtcgag cacctcgggc acgttctccg gcgtgatctg gtcgagcagc tcgccggccc
     3661 acggcagctt cgggtccatg tcgaactcct ggccgagcgc ggtcgccagc gcgatgaatt
     3721 tggcgacgtg cttctccgag gtgatcttgc gcatcgccgc gcgctcgatg ccctgctgca
     3781 ccatatcggc gaggtcgtcc cgggacacgc cgaagcagcc gcccggaaat tgcttgccca
     3841 gctggcggac catccggtcc tcgtagttac gggccgccgt cctggcgatc tctgccatct
     3901 gctgcctggg gatcttgaac atcgcagcac atactcctag catatcccgc gcacacgcga
     3961 aaatgttcgg tgccgcgcga gcccatccgc gtcagatcca ccgcggcgcg gccggctggc
     4021 cggccgctct ttgccggcgg tcacaccgcg gc
//
LOCUS       DASWNJ010000842         1623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_15810, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000842 DASWNJ010000000
VERSION     DASWNJ010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15810"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1460
                     /gene="glmS"
                     /locus_tag="VGB85_14330"
     CDS             <1..1460
                     /gene="glmS"
                     /locus_tag="VGB85_14330"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEY0135257.1"
                     /translation="DTEVVPHLIAAAFAAGAPDLEHAVSQALRSVVGAYGIAVLDADH
                     PDRIVVARHGSPVLLGVGEREMFVASDVSALVGYTRQVVYLDDGELATITAGGYRTFT
                     LDDRSTAKSPATIDWDVIGAEIGDHAHFLQKEIGEQPKAIERALRGRLDERFATAHLG
                     GLNLSVREAREFRRVKILGCGSACYAGDLGAQLIEDLARMPATSESASEFRYRNPVVE
                     PDTLYVAVSQSGETVDTLAAVQELQRKGGRVIGIVNVVGSTIARQVDGGIYLHAGPEM
                     SVAATKSFTSTVTAFALLALHLGRMRDLSPSNGKRLIEALKALPGKVAEVLKLDEEIA
                     AIARELAPHPSMLFIGRRRGCPVAREGAQKLKEISYIHAEAYASSELKHGPLALVSPE
                     MPTVAVVPDDDLLDKNTSTLAEIKARRGPVIAIAHRELPTDRADRTIVVPKSEDELDP
                     ILLSVPLQILAYHAAVALERDVDKPRNLAKSVTVE"
BASE COUNT          243 a          577 c          588 g          215 t
ORIGIN      
        1 ccgacaccga ggtcgtcccg cacctcatcg ccgccgcgtt cgccgcgggc gcgccggacc
       61 tggagcacgc cgtcagccag gcgctgcgca gcgtggtcgg tgcgtacggg atcgcggtgc
      121 tggacgccga ccaccccgac cgcatcgtgg tcgcccggca cggcagcccg gtgctgctgg
      181 gcgtggggga gcgcgagatg ttcgtcgcgt ccgacgtctc ggcgctggtc ggctacacgc
      241 gccaggtcgt ctacctcgac gacggcgagc tggcgacgat caccgcgggc ggctaccgca
      301 cgttcaccct cgacgaccgg tcgacggcca agagccccgc gacgatcgac tgggacgtca
      361 tcggcgccga gatcggcgac cacgcccact tcctgcagaa ggagatcggc gagcagccga
      421 aggcgatcga gcgggccctg cgcgggcggc tcgacgagcg gttcgcgacc gcgcacctgg
      481 gcgggctgaa cctgtcggtg cgcgaggcgc gcgagttccg ccgcgtgaag atcctcggct
      541 gcggctcggc ctgctacgcg ggcgacctgg gagcccagct gatcgaggac ctggcgcgga
      601 tgccggcgac ctcggagtcg gcctcggagt tccgctaccg caacccggtg gtcgagccgg
      661 acacgctcta cgtcgcggtg agccagtccg gcgagaccgt cgacaccctc gccgcggtgc
      721 aggagctgca gcgcaagggc ggcagggtga tcggcatcgt caacgtggtc gggtcgacga
      781 tcgcccggca ggtcgacggc gggatctacc tgcacgccgg cccggagatg tcggtggccg
      841 cgaccaagtc gttcacctcg accgtcacgg cgttcgccct gctggcgctg cacctcgggc
      901 ggatgcgcga cctgtcgccg agcaacggca agcggctgat cgaggccctc aaggcgctgc
      961 ccgggaaggt cgcggaggtg ctgaagctgg acgaggagat cgccgcgatc gcccgcgagc
     1021 tggccccgca cccgagcatg ctgttcatcg gccgccggcg cggctgcccg gtggcgcgcg
     1081 agggggcgca gaagctcaag gagatctcct acatccacgc cgaggcctac gcctccagtg
     1141 agctcaagca cggcccgctg gcgctggtca gcccggagat gccgacggtc gcggtcgtcc
     1201 ccgacgacga cctgctcgac aagaacacct cgacgctggc cgagatcaag gcccgccgcg
     1261 gcccggtgat cgcgatcgcg caccgcgagc tgccgacgga ccgggccgac cggacgatcg
     1321 tggtgccgaa gtcggaggac gagctcgacc cgatcctgct gtccgtgccg ctgcagatcc
     1381 tggcctacca cgcggcggtg gcgctggagc gcgacgtcga caagccccgc aacctggcca
     1441 agagcgtcac ggtcgagtag ccgagcccgc cccggggtgt tcccgcgagt cggcacgttc
     1501 gtcaggccga gtgcgcacgt tcatcaggca tcgagggggc ctggcctgca cattcacgca
     1561 gtcgaacgtg ccgactcgcg ctgttgaacg tgccgactcg cgctgtcgaa ggtgccgact
     1621 cgc
//
LOCUS       DASWNJ010000843         3371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_298772, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000843 DASWNJ010000000
VERSION     DASWNJ010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298772"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VGB85_14335"
     CDS             complement(<1..358)
                     /locus_tag="VGB85_14335"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEY0135258.1"
                     /translation="MMDEHASELDLLQAWRAGVPAAGNALARRHQAGLRRVFAGRVAP
                     QDVEELIQQTWLAMTLATDRLEASGREPLHSFRAYLFGVARNILGAHYRRRQRDVDFD
                     PEVERLGAAEPSPSRQL"
     gene            472..1122
                     /locus_tag="VGB85_14340"
     CDS             472..1122
                     /locus_tag="VGB85_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4291 domain-containing protein"
                     /protein_id="HEY0135259.1"
                     /translation="MAPRLRLAPYLEQRAAWPAAGRHILAQADAETVVVYQAYRPEIG
                     RYTAEYGRFGGGWSRDRMSWIKPNFLWMMYRCAWATSPGQEVVLALWLQRAGFDEILA
                     SAVPSSFDPRRHDSEAAWKLAVKTSDVRLQWDPDHAPGGAPEARRAIQLGLRGEALRS
                     FADRWLLAVEDITPLVRAEHAHASPPFGELSTPAEQVYRPEDPAVAAALGLDPWPA"
     gene            complement(1123..2439)
                     /locus_tag="VGB85_14345"
     CDS             complement(1123..2439)
                     /locus_tag="VGB85_14345"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEY0135260.1"
                     /translation="MTTKEQLDAAASLRARMLAQESVVPTSSLRRLWRTGSSAVGLAR
                     QLVRGRDGEAIDLAAIAEIVGGLGNLKGVAMKVGQMLGHVDPNVPEELRGMLSLLQTA
                     APATAFAAVAAEVRASLGARAEALLAGMERAPVAVASIGQVHRARLPDGTEVAVKVRH
                     PGIEAAIAADFRSAGVGKLFAAVTGAAAIREVIDEAREAFLAECDFRREATNQRAFAR
                     LFDDDPVIVVPAVLEDWCSDRVLVTRWIAGRSLAQFLAAAPAQHERDAAGAALFRFWV
                     RTLYREGLFHADPHPGNFAFCDQGKLVIYDFGCVRRFDAPLRRAFAQLAAATRDDDPA
                     AIAAAITAVGGRVPNDPDGRAHTRALLRGFFAPLLTPGKRRITIDEGMHARELLRDKR
                     ALLGLQLPGRMLFLFRLRFGLYAVLAQLGAEVDWAGLEDSWARAGL"
     gene            2469..3068
                     /locus_tag="VGB85_14350"
     CDS             2469..3068
                     /locus_tag="VGB85_14350"
                     /inference="COORDINATES: protein motif:HMM:NF037625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEY0135261.1"
                     /translation="MRVTPESPGDGRTRRKQEGMARIQAVALDLFEAHGFAEVSIEAI
                     AAAAGAGPATIYRNFGTKERLVLWDEYDPRLLAALTTALAETDVLTAVQQALTRSLAD
                     VYRDERQRILRRARLVRATPALVAASAADQQALRAALASVLRATGHARDELAAQVFAG
                     AIVAAIVAGLDRWLDGDGSEPLTRCFRMALARLRALAGA"
     gene            3146..>3371
                     /locus_tag="VGB85_14355"
     CDS             3146..>3371
                     /locus_tag="VGB85_14355"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEY0135262.1"
                     /translation="MTDAQLSDLQLGLLRILWDRQEATALDVHAAVVAQRRELAPTTV
                     ATMLSRLEKKGLITHRVEGRQYIYRPLVSEP"
BASE COUNT          466 a         1201 c         1258 g          446 t
ORIGIN      
        1 acagctggcg gctcggggag ggctcggcgg cgccgaggcg ctcgacctcg gggtcgaagt
       61 cgacgtcgcg ctggcgccgg cggtagtgcg cgccgaggat gttgcgggcg acgccgaaca
      121 ggtaggcccg gaagctgtgc aacggttcgc ggccgctggc ctcgaggcgg tcggtcgcca
      181 gcgtcatcgc cagccaggtc tgctggatca gctcctcgac gtcctgcggg gcgacgcgac
      241 cggcgaacac gcggcgcagg ccggcctggt ggcggcgcgc gagcgcgttg cctgccgcgg
      301 gcacaccagc gcgccaggcc tgcaggagat cgagctccga cgcgtgttcg tccatcatgg
      361 ccggctcgcg gacaacgccg cgcacgatca ctttactccc cgcgcttgat ctcgcccgga
      421 gcaggcgtga cgtggtgttg actgggtgcc gatcggcgac gacattgctg catggccccg
      481 cgactgcgac tcgcccccta cctcgagcag cgcgccgcgt ggccggcggc ggggcgccac
      541 atcctcgccc aggcggacgc cgagacggtc gtcgtgtatc aggcgtatcg cccggagatc
      601 gggcgctaca cggccgagta cggccgcttc ggcggcggct ggagccgcga tcgcatgtcg
      661 tggatcaagc cgaattttct gtggatgatg taccgctgcg cgtgggcgac gagcccgggg
      721 caggaggtcg tgctcgcgct gtggctgcag cgggccggct tcgacgagat cctcgcgagc
      781 gccgtgccgt cctcgttcga cccgcggcgt cacgactcgg aggccgcgtg gaagctggcg
      841 gtgaagacct ccgacgtgcg cctgcagtgg gaccccgacc acgcgccggg cggcgcgccg
      901 gaggctcgtc gcgcgatcca gctcgggctg cgcggcgagg ccctgcgcag cttcgccgat
      961 cggtggctgc tcgcggtcga ggacatcacg ccgctggtcc gcgccgagca tgcgcacgcg
     1021 agcccgccgt ttggcgagct gagcacgccc gcggagcagg tctatcgtcc ggaggacccg
     1081 gcggtcgcgg cggcgctcgg cctcgatccc tggccggcgt gatcacagcc cggcgcgcgc
     1141 ccagctgtcc tcgaggccag cccagtcgac ctccgcgccg agctgcgcga gcacggcgta
     1201 gaggccaaag cgcaggcgaa acaggaacag catgcgcccg ggcaattgca ggccgagcag
     1261 ggcccgcttg tcgcgcagca gctcgcgggc gtgcatgccc tcgtcgatcg tgatccgccg
     1321 ctttccgggg gtcagcagcg gggcgaagaa gccgcgcagc agggcccgcg tgtgcgcgcg
     1381 gccgtcgggg tcgttcggga cgcgaccgcc cacggccgtg atcgcggcgg cgatcgccgc
     1441 cgggtcgtcg tcgcgggtcg ccgcggccag ctgcgcgaac gcccggcgca gcggcgcgtc
     1501 gaagcggcgc acgcagccga agtcgtagat gacgagcttg ccctgatcac aaaacgcgaa
     1561 gttgccgggg tgggggtcgg cgtggaacag gccctcgcgg tagagcgtgc gcacccagaa
     1621 gcggaacagg gccgcgccgg cggcgtcgcg ctcgtgctgc gccggcgcag cggcgaggaa
     1681 ctgcgcgagc gagcggccgg cgatccagcg ggtgacgagc acgcggtcgc tgcaccagtc
     1741 ctccagcacc gccggcacga cgatcaccgg gtcgtcgtcg aacagccgcg cgaacgcccg
     1801 ctggttggtc gcctcgcggc gaaagtcgca ctcggcgagg aaggcctcgc gcgcctcgtc
     1861 gatcacctcg cggatcgccg cggcgccggt gaccgcggcg aacagcttgc cgacgcccgc
     1921 gctgcgaaag tcggcggcga tcgccgcctc gatgccgggg tggcgcacct tgaccgcgac
     1981 ctcggtgccg tccggcaggc gcgcgcggtg gacctggccg atgctggcga ccgcgaccgg
     2041 ggcccgctcc atgccggcga gcagcgcctc cgcgcgcgcg ccgagggagg cccgcacctc
     2101 ggccgcgact gccgcaaagg cggtcgcggg cgcggcggtc tggagcagcg agagcatgcc
     2161 gcgcagctcc tccggcacgt tggggtcgac gtgaccgagc atctgcccga ccttcatcgc
     2221 cacgcccttg aggttgccga gcccgccgac gatctcggcg atcgcggcga ggtcgatcgc
     2281 ctcgccgtcg cgcccgcgga ccagctgccg ggccaggccg accgcgctgc tgccggtgcg
     2341 ccagagccgt cgcagcgacg aggtcggcac cacggactcc tgcgcgagca tccgcgcccg
     2401 caagctcgcc gcggcgtcaa gctgttcttt cgtcgtcatc gcttctccaa tgtgatagtg
     2461 actatcatgt gagagtcact cccgaaagtc caggggacgg ccggacccgg cgcaagcagg
     2521 agggcatggc gcggatccag gcggtcgcgc tcgacctctt cgaggcccac ggcttcgcgg
     2581 aggtctcgat cgaggcgatc gcggcggcgg ccggggccgg cccggcgacc atctatcgca
     2641 acttcggcac caaggagcgg ctggtgctgt gggacgagta cgaccccagg ctgctcgcgg
     2701 cgctgacgac ggcgctcgcc gagaccgacg tgttgacggc cgtgcagcag gcgctgacgc
     2761 gctcgctcgc cgacgtctac cgcgacgagc ggcagcggat ccttcgccgc gctcgcctgg
     2821 tccgcgcgac cccggcgctg gtcgccgcgt cggccgcgga tcaacaggcg ctgcgcgccg
     2881 cgctggcgag cgtgctgcgg gcgaccggcc acgcgcgcga cgagctcgcg gcgcaggtgt
     2941 tcgcgggggc gatcgtggcg gcgatcgtgg ccggcctcga ccgctggctc gacggcgacg
     3001 gcagcgaacc actgacccgc tgctttcgca tggcgctcgc ccgcctgcgc gcgctcgccg
     3061 gggcttgaaa aagcgcaggc gctgaaattc ggccgcaccg ggctcttgac gagcaaacta
     3121 cacgtgtagt attacacctg tagtcatgac cgacgcgcag ctaagcgact tacagctcgg
     3181 cctcttgcgg atcctctggg atcggcagga ggccaccgcc ctcgatgtcc acgcggccgt
     3241 ggtcgcccag cgccgggagc tggcgccgac gacggtcgca accatgctct cgcggctgga
     3301 gaagaagggc ctgatcacgc atcgcgtcga gggccggcag tacatctacc gcccgctggt
     3361 cagcgaaccg g
//
LOCUS       DASWNJ010000844         1522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_546445, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000844 DASWNJ010000000
VERSION     DASWNJ010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546445"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1449
                     /locus_tag="VGB85_14360"
     CDS             <1..1449
                     /locus_tag="VGB85_14360"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0135263.1"
                     /translation="RADDFDGAIKALGRVELDIPTRKLVEFLDSARGKQKTAGPLLTL
                     AAQFIPDDDEDSGRIPPSFITQGWGIVENLARRATERYPDDAFAHLLLARSFRQQGLV
                     DAAVVHLDRTVEIKKDIFDAWQDLAVLKQSSLERLAGVDADAAKARLPELEKFHAQAV
                     ELWSGRPIRPGLSEAYYTVAQGLYELGRVDEAKHLLGKSVAREPVAEALDLLATIEAK
                     SGQKTEAAGHYEAILGLPFADQRTRLRWETQARTALAQIRRRQGDEAEARTQFTTALR
                     QLNKLIGYPALGARERSDRLIERGKLLFYLGDPEQGADDFRQAMSVTPDRAEAYAEPM
                     LFVVNHGYYELALEIFRQALPRDEVRGSLKLYFSLWLHELALRQNKPQDPAVVEYLQG
                     FADNSWQGKLARHAQGKLSYEELLRGATDSGEQAEAHFYEALRRWRSSPGDAKQLLRK
                     VIDTHRMAFFEYDMAQNYLEWNELPAAARPPR"
BASE COUNT          256 a          604 c          467 g          195 t
ORIGIN      
        1 cgcgccgacg acttcgacgg ggcgatcaag gccctcggcc gcgtcgagct cgacatcccc
       61 acgcgcaagc tcgtcgagtt cctcgactcc gcccgcggca aacaaaagac cgccggcccg
      121 ctgctgaccc tcgccgccca gttcatcccc gacgacgacg aggacagcgg acggatcccg
      181 ccatcgttca tcacgcaggg ctggggcatc gtcgagaacc tcgcccgccg cgcgaccgag
      241 cgctaccccg acgacgcctt cgcccacctg ctgctcgccc gcagctttcg ccagcaaggc
      301 ctcgtcgacg ccgccgtcgt ccacctcgac cgcaccgtcg agatcaaaaa agacatcttc
      361 gacgcctggc aagacctcgc ggtcctcaag cagagcagcc tcgagcgcct cgccggcgtc
      421 gacgccgacg ccgcgaaggc caggctgccc gagctcgaga agttccacgc ccaggccgtc
      481 gagctgtggt ccggccggcc gatccgaccc gggctgtccg aggcctacta cacggtcgcc
      541 cagggcctct acgagctcgg ccgcgtcgac gaggccaagc acctgctcgg caagagcgtc
      601 gcccgtgagc ccgtcgccga ggccctcgac ctgctcgcca cgatcgaggc caagagcggg
      661 caaaaaaccg aggccgccgg gcactacgag gcgatcctcg gcctgccctt cgccgaccag
      721 cgcacccgcc tgcgctggga gacccaggcc cgcaccgcgc tggcccagat ccgccgccgc
      781 cagggcgacg aggccgaggc ccgcacgcaa ttcacgaccg ccctgcgcca gctcaacaag
      841 ctgatcggct acccggcgct cggcgcccgt gaacgcagcg accgcctgat cgagcgcggc
      901 aagctgctgt tctacctcgg cgacccggag cagggcgccg acgacttccg ccaggcgatg
      961 tcggtcaccc ccgaccgcgc cgaggcctac gccgagccga tgctgttcgt cgtcaaccac
     1021 ggctactacg agctcgccct cgagatcttc cgtcaggccc tcccgcgcga cgaggtccgc
     1081 ggctcgctca agctgtactt cagcctgtgg ctgcacgagc tcgccctgcg ccaaaacaag
     1141 ccgcaagacc ccgcggtcgt cgagtacctg caaggcttcg ccgacaactc ctggcaaggc
     1201 aagctcgccc gccacgcgca gggcaagctc tcgtacgagg agctgctgcg cggcgcgacc
     1261 gactcgggcg agcaggccga ggcccatttt tacgaggcgc tgcgccgctg gcgtagctcc
     1321 ccgggcgacg ccaagcagct cctgcgcaag gtgatcgaca cccaccgcat ggccttcttc
     1381 gagtacgaca tggcccagaa ctacctcgag tggaacgagc tgcccgcggc cgctcgcccg
     1441 ccgcggtgac gcgggggctc gggacgtgtt catcgactga tgctcggcac cggagcgtcc
     1501 tcccgcggcc tcgaacgtcg ct
//
LOCUS       DASWNJ010000845         2931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_367976, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000845 DASWNJ010000000
VERSION     DASWNJ010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367976"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1198)
                     /locus_tag="VGB85_14365"
     CDS             complement(<1..1198)
                     /locus_tag="VGB85_14365"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0135264.1"
                     /translation="MRRASLLLLFTACQGGIVASESEGGTSTSTSTSASPTTSAASFA
                     TSTTGAGTGGGTSGAGSTGAGEEATGAPPTTDTGDPTGEPPPLHTLHFAVIGDYGLDG
                     TATGQVAGRVASWNPEIVLTTGDNNYYSGLAKTIDANIGKYYRGFIAPYVGTYGPGAA
                     ENRFFPCPGNHDWNSGTLEPYLDYFTLPGNERYYAVRRGPVEFFMLDSDYHEPDGLTP
                     TQVQGQWLEAALAESTASFQVVVMHHAPYSSGYHGGTKAMRWPYAAWGADLVLAGHDH
                     NYERFVVDGLDYVVTGTGGAALRKFVTEEPGSQRGHANGFGALRGEANEERMTLDFIG
                     VNGGRIDRLTIAADPPKLWQVRVAANATWRWRETPPGAGWDEPDFADDDWAAGPAPLG
                     FGAGGEA"
     gene            complement(1334..>2931)
                     /locus_tag="VGB85_14370"
     CDS             complement(1334..>2931)
                     /locus_tag="VGB85_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135265.1"
                     /translation="ALYLTDQWPLLSRAPRPVEGELAARLRALLAARGASFFADLAAE
                     TGAFPADLLTALWDLVWAGEATNDTLAPLRKIVHGSVSDTRRRPVPQGLFRSRRLGPP
                     GSEGRWSLLPRPAAPVQKGMSEGTGAKGHGTLGMSEWTGAKGQGTSAMSSRTGGSDMA
                     AGTGLKGQGTSAMSVGTGLKGQGAPDMSLGTGWKGQGAKGMSVGTGAKGQGTPGMSLR
                     TGAKGQGASGMSVGTGVELDATGQPATVARGPATGGAGRGGVGLGGDVTGSDTTGVRE
                     SDASGLAGDVPGAEADIGPAIGVPSEPIAPELTPAEGAALLASLGPSGTARAAALARV
                     LLERYGVLTREAVHAEDIAGGFSAVYAVLGAMEQAGKIRRGYFVAGLGGAQFALAGAD
                     ERLRALREPGESPEICVLATTDPCNPYGAALPWPERPGTRLARAAHTRVVLVDGALAA
                     YLTRDARSVWTFLPEEEPERSQMTAALARALAGLVGGQRRTLVLGQIDGVDAQVSPLA
                     PALERAGFAMTSQGLFIRAHDEN"
BASE COUNT          368 a         1159 c          989 g          415 t
ORIGIN      
        1 tcgcctcgcc gccggccccg aagcccagcg gcgcgggccc ggccgcccag tcgtcgtcgg
       61 cgaagtcggg ctcgtcccag ccggcgcccg gcggggtctc gcgccagcgc cacgtcgcgt
      121 tcgcggcgac ccgcacctgc cacagcttcg gggggtcggc cgcgatcgtc aggcggtcga
      181 tgcgtccgcc gttgaccccg atgaagtcga gcgtcatgcg ctcctcgttc gcctcgccgc
      241 gcagcgcccc gaagccgttg gcgtggccgc gctgcgagcc gggctcctcg gtcacgaact
      301 tgcgcagcgc cgcgccgccg gtgccggtca cgacataatc gagaccatcg acgacgaagc
      361 gctcgtagtt gtggtcgtgc ccggcgagca ccaggtcggc gccccaggcc gcgtacggcc
      421 agcgcatcgc cttggtcccg ccgtgatagc ccgacgagta cggcgcgtgg tgcatcacga
      481 ccacctggaa gctcgcggtc gactcggcga gcgccgcctc gagccactgg ccctgcacct
      541 gcgtcggcgt caacccgtcg ggctcgtggt agtcgctgtc gagcatgaag aactcgaccg
      601 gcccgcggcg taccgcgtag tagcgctcgt tgcccggcag cgtgaaataa tcgaggtacg
      661 gctcgagggt cccgctgttc cagtcgtggt tcccggggca cggaaagaag cgattttcgg
      721 ccgcgccggg cccgtaggtg ccgacgtacg gcgcgatgaa gccgcggtag tacttgccga
      781 tgttggcgtc gatcgtcttc gccaggccgc tgtaataatt attgtcgccg gtcgtgagca
      841 cgatctccgg gttccagctg gcgacacgcc cggcgacctg gcccgtcgcc gtgccgtcga
      901 gcccgtagtc gccgatcacc gcgaagtgca gcgtgtgcag cggcggcggc tcgccggtcg
      961 gatcgccggt gtcggtcgtc ggcggagcgc cggtcgcctc ctcgcccgcg ccggtgctcc
     1021 cggccccgct cgtgccaccg cccgtgcccg cgcccgtcgt actcgtggcg aagctcgccg
     1081 cgctcgtcgt cgggctcgcg ctcgtgctcg tgctcgtgct cgtgccgccc tcggactcgc
     1141 tggcgacgat gcctccctga caggcggtga acagcagcag cagactggcc cggcgcatcg
     1201 ctcccacgat acacgcgacc gcgagggtgt aggcggtgcg gaccgccgcc gctgccgacc
     1261 tgcaggcgtc cccgcggcac ggcgcgcccc tcgtcagttc ccgtcagttc tcgtcaattc
     1321 tcgtcaattc tcatcaattt tcatcatggg cgcggatgaa gaggccctga ctcgtcatcg
     1381 caaagccggc ccgctcgagc gccggcgcga gcggcgacac ctgggcgtcg acgccgtcga
     1441 tctgcccgag cacgagggtg cgccgctgcc cgccgaccag ccccgcgagc gcgcgcgcca
     1501 gcgcggccgt catctgcgag cgctcgggtt cctcctccgg gaggaaggtc cacaccgagc
     1561 gcgcatcgcg ggtcaggtac gccgcgagcg ccccgtcgac cagcaccacg cgcgtatggg
     1621 ccgcgcgcgc gaggcgggtc cccgggcgct cgggccacgg caacgcggcc ccgtaggggt
     1681 tgcacgggtc ggtggtcgcc agcacgcaga tctcggggct ctcgcccggc tctcgcaggg
     1741 cccgcagccg ctcgtcggcg ccggcgagcg cgaactgggc cccgccgagc cccgcgacga
     1801 agtagccgcg ccggatcttg cccgcctgct ccatcgcccc gagcaccgcg tagaccgccg
     1861 agaacccgcc cgcgatgtcc tccgcgtgca ccgcctcgcg cgtcagcacg ccgtagcgct
     1921 cgagcagcac ccgcgcgagc gcggcggccc gggccgtgcc cgacggcccg aggctggcca
     1981 gcagcgcggc gccctcggcc ggcgtcagct cgggcgcgat cggctcggag ggcacaccga
     2041 tcgccggccc gatgtccgcc tcggccccgg ggacgtcgcc ggcgagcccc gacgcatcgc
     2101 tctccctcac gcccgtcgta tcgctccccg tcacgtcccc gccgagaccc accccgcccc
     2161 gccccgcccc gcccgtcgcc gggccccgtg cgacggtcgc gggctggccc gtggcgtcca
     2221 gctcgacgcc tgtcccgacg gacatgcccg aagcgccctg gcccttcgcg cctgttcgga
     2281 gcgacatgcc cggagtaccc tgtcccttcg cgcctgtccc gacggacatg cccttcgcgc
     2341 cctgcccctt ccagcctgtc cctagcgaca tgtccggagc gccctgtccc ttcagacctg
     2401 tcccgacgga catggccgaa gtaccctgtc ccttcagtcc tgtcccggca gccatgtccg
     2461 aaccacctgt cctcgaggac atggccgaag taccctgtcc cttcgcacct gtccattcag
     2521 acatgcccaa agtaccatgt cccttcgcac ctgtcccttc agacatgccc ttttgcacag
     2581 gcgcggccgg ccgcggcagc agcgaccagc gcccctcgct gccgggcggg ccgagccggc
     2641 gcgagcggaa cagcccctgc gggaccggcc ggcgccgcgt gtcggacacg ctgccgtgga
     2701 cgattttacg cagcggcgcg agcgtgtcgt tggtcgcctc gccggcccac acgaggtccc
     2761 acagggccgt cagcaggtcg gccgggaacg cgccggtctc ggcggcgagg tcggcgaaga
     2821 agctcgcgcc gcgggccgcc agcagcgccc gcaggcgggc cgccagctcg ccctcgaccg
     2881 gcctcggcgc gcgcgacagc agcggccact gatcggtcag gtacagcgcg a
//
LOCUS       DASWNJ010000846         3816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_192749, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000846 DASWNJ010000000
VERSION     DASWNJ010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192749"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VGB85_14375"
     CDS             <1..328
                     /locus_tag="VGB85_14375"
                     /inference="COORDINATES: protein motif:HMM:NF024584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HEY0135266.1"
                     /translation="FHVELCNEWGSAGVDDRHYRDQPFLCGAGRISCVIGASGEVLPC
                     TTTDLGESEGNLRERPLREIWTQGFQRFRQGGQGVCSDGKECWLQSRNGNACREQAFG
                     CGRKAG"
     gene            332..1516
                     /locus_tag="VGB85_14380"
     CDS             332..1516
                     /locus_tag="VGB85_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135267.1"
                     /translation="MGRVRSLWVWAGATWVALTIVMRVARGEVVVLSAHAAPVVRLVA
                     TLLVFLAGCAEKLQPQGSAEDAPKDMSQRPAVPAPAPVFELPAGLDDAGLERARAWAG
                     RRVLWSQFSGEVAPDVAPPEHAAPDPAITGAVEAFVAAAAGHVARARSNVPETAASLT
                     ALLDAAEAFHLYDAWLAAHLWAHTRALRPAPGPLYARIDRHLRVCDAISQAEFTTGPI
                     EFSAWRSKAGPPPGWRPEVRVPAGLVAAARAGFARATAGSFESDHVLTLTVARGEVVW
                     IHGGAEQRVPAGSSLRLRRIDLVRAPSGAALRHDKLGELVLRPGAELVAWTVGDFVAP
                     AKRAALRGAVERALAGDGAALDEVEATLPAIHDEVRAAIAGQPGAPGAAGLRLVLENF
                     AG"
     gene            complement(1531..>3816)
                     /locus_tag="VGB85_14385"
     CDS             complement(1531..>3816)
                     /locus_tag="VGB85_14385"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0135268.1"
                     /translation="VSLYEALYGQPPFDATSLLALTYAVTQGKVREPPAGVAVPTALL
                     QIVLRGLSVNPNKRFASMPALLGALERTLERRRVPWFVVAGVAGMIGAAGFTAASLRP
                     GEDTCAAVSGELIGMWDAPSSAAARQGVLATKVPFAEDTWARVQTRLDTYARDLGEMR
                     VDACRAHAEGRSSMRMFDLRTACLDQRHASLAAFVAILKTADAEVVGNAAAAAAALPL
                     IAGCGDTRALTEAVAPPEDPESITRVAAARGVLAEAQAHELSGQFARGLELVDGIDLR
                     GLDYPPLQAEVGRRRGSLLSEAGRHPEAITELTDALRIAMASGHDLEAAAIATRRDFV
                     RAARLGQGREVLDDAPLVEGLVGRVESTRVGAEYRGDHLNNLGIAHAVLGELQRAPEF
                     FAASIDARRAVLGEDHPQVVYALGNLGLALVTGDNVVEGARRLRTAFLAAESTLGPKH
                     PHIALLAVNLGYGHEVLRQYRDAATYFERGLKLQTELLGPDSPDLTYVLRALGILALD
                     QRRCDEANESFTRALRVIGATPETDNPAALDTLVGLGNAAICRGDTDVGRAHLERALA
                     LGEQVHGAEDLNITEVLDALTDMYLRTGDLDQALLLGQRSLAIRRTKLPANSTRLSQS
                     YRRIAEVHRRAHRFGEAATALQLAQALHDARPVIESIESAKIRLQLGDLMLERGEAAA
                     ARGHYERAVAIYAPIVDPDVLELALSRYGLARAMSAQAGTVTPDARALAEQALTTLQA
                     KGPAFAPEQRAVRAWLARPAK"
BASE COUNT          442 a         1338 c         1543 g          493 t
ORIGIN      
        1 gttccacgtc gagctgtgca acgagtgggg cagcgccggg gtcgatgacc ggcactatcg
       61 cgaccagccg ttcctgtgcg gggccgggcg gatctcgtgc gtgatcgggg cgagcggcga
      121 ggtgctgccg tgcacgacca ccgacctcgg cgagagcgag ggcaacctgc gcgagcgacc
      181 gctgcgcgag atctggacgc agggcttcca gcggttccgc cagggcgggc agggcgtctg
      241 ctccgacggc aaggagtgct ggctgcagag ccgtaacggc aacgcctgcc gcgagcaggc
      301 gttcggctgc gggcgaaagg cggggtgatc gatgggccgg gtccgatcgc tgtgggtgtg
      361 ggcgggcgcg acgtgggtcg cgctgacgat cgttatgcgg gtcgcccgtg gcgaggtcgt
      421 ggtgctgtcc gcccacgccg cgccggtggt gcggctcgtc gccacgctgc tcgtgttcct
      481 cgccggctgc gccgagaagc tgcagccgca ggggtccgcc gaggatgccc cgaaggacat
      541 gtcacaaagg ccagccgtgc cggcgccagc gccggtgttc gagctcccgg cgggcctcga
      601 cgacgccggg ctcgagcggg cgcgcgcgtg ggccggccgg cgcgtgctgt ggtcgcagtt
      661 ctccggcgag gtggcgcccg acgtcgcgcc cccggagcac gccgcgcccg atccggcgat
      721 cacgggcgcg gtcgaggcct tcgtcgcggc ggcggccggg cacgtcgcgc gggcccggtc
      781 gaacgtgccc gagaccgccg cgagcctgac ggcgctgctc gacgcggccg aggcgtttca
      841 cctgtacgac gcctggctgg cggcgcacct gtgggcgcac acgcgggcgc tgcgacccgc
      901 gccggggccc ctgtatgccc ggatcgaccg ccacctgcgg gtgtgcgacg cgatctcgca
      961 ggccgagttc accaccgggc cgatcgagtt ctcggcgtgg cgatcgaagg ccgggccgcc
     1021 gcccggctgg cgacccgagg tgcgcgtgcc cgcgggcctc gtcgcggcgg cgcgggccgg
     1081 ttttgcgcgc gcgaccgccg gcagcttcga gtcggaccac gtgctcacgc tgaccgtcgc
     1141 gcgcggcgag gtcgtgtgga tccacggcgg cgccgagcag cgggtgcccg ccgggtcgtc
     1201 gctgcggctg cgccggatcg acctcgtgcg cgcgccgtcc ggcgcggcgc tgcgccacga
     1261 taagctcggc gagctggtgc ttcgtcccgg cgccgagctg gtggcgtgga cggtcggcga
     1321 cttcgtggcg ccggcgaagc gagccgcgct gcgcggcgcg gtcgagcggg cgctcgcggg
     1381 cgacggcgcg gcgctcgacg aggtcgaggc cacgttaccg gcgatccacg acgaggtccg
     1441 cgccgcgatc gcgggccagc ccggcgcgcc cggggccgcg ggtctgcgcc tcgtgctcga
     1501 gaacttcgcc ggctgacccg gcgcgccgcg tcacttggcc gggcgcgcga gccaggcgcg
     1561 cacggccctc tgctcgggcg cgaaggcggg gcccttggcc tgcagggtcg tcagggcctg
     1621 ctccgcgagg gcgcgcgcgt cgggcgtgac ggtgccggcc tgggcgctca tcgcccgcgc
     1681 caggccgtag cgggacagcg cgagctctag cacgtccggg tcgacgatcg gcgcgtagat
     1741 cgcgacggcg cgctcgtagt ggccgcgggc cgccgccgcc tcgccgcgct cgagcatcag
     1801 gtccccgagc tgcaggcgga tcttcgccga ctcgatgctc tcgatcaccg gccgggcgtc
     1861 gtgcagcgcc tgcgccagct gcagcgccgt cgcggcctcg ccgaagcggt gcgcgcggcg
     1921 gtggacctcg gcgatccggc ggtacgactg gctgaggcgg gtcgagttgg ccgggagctt
     1981 cgtgcgccgg atcgccaggc tgcgctggcc gaggagcaac gcctggtcga ggtcgcctgt
     2041 ccggaggtac atgtccgtca gggcatccag gacctcggtg atgttgaggt cctcggcgcc
     2101 gtggacctgc tcgccgagcg cgagggcccg ctcgaggtgc gcgcggccga catcggtgtc
     2161 gccgcggcag atcgcggcgt tgccgaggcc gaccagcgtg tcgagggccg cggggttgtc
     2221 ggtttcgggg gtcgcgccga tcacccgcag cgcgcgggtg aagctctcgt tcgcctcgtc
     2281 gcagcggcgc tggtcgagcg ccaggatgcc gagcgcccgc agcacgtacg tcaagtccgg
     2341 cgagtcgggc ccgagcagct cggtctgcag cttcaggccg cgctcgaagt aggtcgccgc
     2401 gtcgcgatac tggcgcagga cctcgtggcc gtagccgagg ttgaccgcca gcagggcgat
     2461 gtgcgggtgc ttggggccga gggtcgactc ggccgcgagg aacgcggtgc gcaggcgccg
     2521 ggcgccctcg acgacgttgt cgccggtgac gagcgcgagg ccgaggttgc cgagcgcgta
     2581 gacgacctgc gggtggtcct cgccgagcac ggcccggcgc gcgtcgatcg acgcggcgaa
     2641 gaactcgggg gcgcgctgga gctcgccgag caccgcgtgg gcgatgccga ggttgttgag
     2701 gtggtcgccg cgatactcgg cgccgacgcg ggtcgactcg acgcgcccga ccaggccctc
     2761 gacgagcggc gcgtcgtcga ggacctctcg accctggccg agccgggcgg cgcgcacgaa
     2821 gtcgcgacgc gtggcgatcg cggccgcctc gaggtcatgg ccgctggcca tggcgatccg
     2881 cagcgcgtcg gtcagctcgg tgatcgcctc ggggtggcgt ccggcctcgc tgagcaggct
     2941 gccgcgccgg cgcccgacct cggcctggag cggcggatag tcgaggccgc gcaggtcgat
     3001 gccgtcgacg agctcgaggc cgcgggcgaa ctgccccgag agctcgtggg cctgcgcctc
     3061 cgcgagcacg ccgcgggccg ccgcgacgcg ggtgatgctc tcggggtcct cgggcggcgc
     3121 gacggcttcg gtcagcgccc gggtgtcgcc gcagccggcg atcaagggca gcgcggcggc
     3181 ggcggcggcc gcgttgccga cgacctcggc gtcggccgtc ttgaggatcg ccacgaaggc
     3241 ggcgaggctc gcgtggcgct ggtcgaggca cgcggtgcgc aggtcgaaca tgcgcatcga
     3301 cgagcggccc tccgcgtgcg cgcggcaggc gtcgacgcgc atctcgccga ggtcgcgcgc
     3361 gtaggtgtcg aggcgggtct gcacgcgggc ccaggtgtcc tcggcgaacg gcaccttggt
     3421 ggcgagcacg ccctggcgcg cggccgcgga ggacggcgcg tcccacatgc cgatcagctc
     3481 gcccgagacc gcggcgcagg tgtcctcgcc cggtcgcagc gacgcggccg tgaagccggc
     3541 cgcgccgatc atgccggcga cgccggcgac gacgaaccat ggcacgcgcc ggcgctcgag
     3601 cgtgcgctcg agcgcgccga gcagcgcggg catcgacgcg aagcgcttgt tcgggttgac
     3661 cgacagcccg cgaagcacga tctgaaggag agcggtcggc accgcgacgc ccgcgggcgg
     3721 ctcgcggacc ttgccctgcg tcaccgcgta ggtgagcgcc agcagcgagg tcgcgtcgaa
     3781 cggcggctgg ccgtacagcg cctcataaag gctgac
//
LOCUS       DASWNJ010000847         4133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_263501, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000847 DASWNJ010000000
VERSION     DASWNJ010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263501"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VGB85_14390"
     CDS             complement(<1..781)
                     /locus_tag="VGB85_14390"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY0135269.1"
                     /translation="MLMGLLGAACGPVAAPGNDTGGETGTTSTSTDPTTTSPTTTDPS
                     TSNDPSDPSDPSDPSDPSNPSDPTATTQNLECQTDEDCGDMYCGYCSDGYCESGPGCC
                     GVLGGVTPAHAKGWRCEPYYECFTDADCDAYQACEFNACAPISIVPLAPCKPLALTTS
                     EWNLSETPGAFVLADLDGDMDLDLAVAEPAVAQIEIALNDGAGNFKLAGAFPVGLPSD
                     HLSIAAGDLDLDGDVDLAVARQADGSLVLLFGQDAVFTPAPA"
     gene            complement(881..1510)
                     /locus_tag="VGB85_14395"
     CDS             complement(881..1510)
                     /locus_tag="VGB85_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135270.1"
                     /translation="MRAAPLRLMIYDRTCTGPRMRPGLSHAWMTGGLLYGHMLRRLHA
                     WHGARTWAEALAWVAQSEPERPIAEVQFWGHGKWGRAFIDRDPLDSAALEPGHPLHAS
                     LCQIRERLVGPEALWWFRTCETFGAVPGQDFARRLTDFLGCRAAGHTYIIAGWQSGLH
                     SLRPGEVPGWSLTEGLKGGSPEDPREALWSRRREPNTIHCLSGQVPSGF"
     gene            1596..2315
                     /locus_tag="VGB85_14400"
     CDS             1596..2315
                     /locus_tag="VGB85_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135271.1"
                     /translation="MRIERLRDGEVRPDAPAIHHESNGVFGFSWPRTSAVLGAMLGML
                     VLVLAAPALARRLLAKEGPVELASHVVLLTAVLTWLALAWRTGGADRLRAALMTGFLA
                     LVLAEELDWGGVLGLPAIGLRLDAAVGHRNLHNASHGASYLLFGLPLLLHFAAPARMH
                     GGLAPVADERAACFAVALAFIAWNLGPWESHAQELLEGVLYSLLLAAGVRLMRARRDH
                     GVARAGCSSTPTSARRTRPRA"
     gene            2354..3214
                     /locus_tag="VGB85_14405"
     CDS             2354..3214
                     /locus_tag="VGB85_14405"
                     /inference="COORDINATES: protein motif:HMM:NF013575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HEY0135272.1"
                     /translation="MTVLVRLAYDGTDFHGYARQLARPGHPEIRTVQGVLEAALVEVY
                     RQPVVTRAASRTDAGVHALGQVVGWEPPHPIPARGLMLGLLARVPRDVGVLAVWEAAL
                     PDGTPVDPRFWNLGKRYRYRIRTTPRRVPQTARGEWHLPRVLDVAAMQAAAGHFAGEH
                     DFAGFRASACQSRTTVRRLHAVTVTGRPSGDELPDDPRCEPDDGRAEVIEIDVRGEAF
                     LQNMVRIIVGTLVEVGLGRRAPDSIAALLQHPDRRRSGATAPPQGLTLVEVLWPSTWP
                     PADGVAALDL"
     gene            complement(3228..3689)
                     /locus_tag="VGB85_14410"
     CDS             complement(3228..3689)
                     /locus_tag="VGB85_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135273.1"
                     /translation="MRASHVAREIDRDERVVLLALRRRLLALLRPVLAPGAPRELPAV
                     LDALDDAVAGARAQARRLNDARIVRRGERPQRTRSPRLPAALAGHACHGEYLGAFPDL
                     AAVARMALGTCPREQMEGASTRLTDLALDLHLRGVLWTLDHDGEVHVFRCR"
     gene            3814..>4133
                     /locus_tag="VGB85_14415"
     CDS             3814..>4133
                     /locus_tag="VGB85_14415"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0135274.1"
                     /translation="MSLPQRPRAVITGAGGGLGRALSLELARRRGRVLAADIDLDAAE
                     ITAAAVRDAGGEAVAVACDVSQQAQVEALPGQADAAFGGTDLIINNAGVAAAGPMAAI
                     PVAD"
BASE COUNT          508 a         1588 c         1487 g          550 t
ORIGIN      
        1 gcgccggggc gggcgtgaac acggcgtcct ggccgaacag cagcacgagg ctgccgtccg
       61 cctggcgcgc gacggcgagg tcgacgtccc cgtcgaggtc gaggtccccg gcggcgatcg
      121 acaggtggtc gctcggcagg ccgaccggga aggcgcccgc gagcttgaaa ttaccggcgc
      181 cgtcgttgag cgcgatctcg atctgcgcca ccgccggctc ggcgaccgcg aggtcgaggt
      241 ccatgtcgcc gtcgaggtcg gcgagcacga aggcgcccgg cgtctcgctc aggttccact
      301 cgctcgtcgt cagcgccagc ggcttgcacg gcgccagtgg gacgatcgag atcggcgcgc
      361 aagcgttgaa ctcgcacgcc tggtaggcgt cgcagtccgc gtcggtgaag cactcgtagt
      421 acggctcgca gcgccagccc ttcgcgtgcg ccggcgtgac tcccccgagc acgccgcagc
      481 agcccgggcc cgactcgcag tagccgtccg agcagtaacc gcagtacatg tcgccgcagt
      541 cctcgtcggt ctgacactcg aggttctgcg tcgtcgccgt cgggtcgctc ggattgctcg
      601 ggtcgctcgg gtcgctcggg tcgctcgggt cgctcgggtc gttcgaggtg ctcgggtccg
      661 tcgtcgtcgg gctcgtcgtc gtcgggtccg tgctcgtgct cgtcgtgcca gtctcaccgc
      721 cggtgtcgtt gcccggcgcg gccaccgggc cgcaggcggc gcccagcagg cccatgagca
      781 tccatgaatt gtcggctcgc ttaatcaagt cagtcataat ccgccgagga tgccagggcg
      841 cccgcgggcc ggcaagcccg accggcccga cggccctcgc tcagaagccg gacggaacct
      901 gtcccgagag acagtggatc gtgttcggct cgcggcggcg ggaccacagc gcctcgcgcg
      961 ggtcctcggg cgaaccacct tttagaccct cggtcagcga ccagcccggg acctcgccgg
     1021 ggcgcaggct gtgcaggccg ctctgccagc cggcgatgat gtacgtgtgc ccggccgcgc
     1081 ggcagccgag gaagtcggtc agccgccgcg cgaagtcctg ccccggcacc gccccgaagg
     1141 tctcgcaggt gcgaaaccac cacagcgcct cgggcccgac cagccgctcg cggatctggc
     1201 acagcgacgc gtgcagcggg tgcccgggct cgagcgcggc gctgtcgagc gggtcacgat
     1261 cgatgaacgc gcgcccccac ttgccatgcc cccagaactg cacctcggcg atcggccgct
     1321 ccggctccga ctgcgcgacc caggcgagcg cctcggccca cgtgcgcgcc ccgtgccacg
     1381 cgtgcagccg tcgcagcatg tgcccgtaca gcaacccgcc ggtcatccac gcgtgcgaca
     1441 gccccggccg catccgcggc ccggtgcagg tccgatcgta gatcatcagg cgcaggggag
     1501 cggctcgcat cgccgcgaca gtgtagcgcg gacgcgacgc acaggccggc gcgaacttgc
     1561 ccccgcgggc cgtggagggc taaactcgcc gcttcatgcg gatcgagcgc ctgcgcgacg
     1621 gcgaggtccg tccggacgct cccgcgatcc accacgagtc gaacggagtg ttcgggttca
     1681 gctggccgcg cacgtcggcg gtgctcggcg ccatgctcgg catgctcgtg ctcgtgctcg
     1741 ccgcacccgc gctcgcgcgc cgcctgctcg ccaaggaggg gccggtcgag ctcgcctcgc
     1801 acgtcgtgtt actgaccgcg gtgctcacgt ggctggcact cgcctggcgg accggcgggg
     1861 ccgaccgact gcgcgccgcg ctgatgaccg gcttcctcgc gctcgtgctc gccgaggagc
     1921 tcgactgggg cggcgtgctc ggcctgcccg cgatcgggct gcgcctcgac gccgcggtcg
     1981 gacaccgcaa cctgcacaac gcctcgcacg gcgcctcgta tctgctgttc gggttgccgc
     2041 tgctgctgca cttcgccgcc ccggcgcgaa tgcacggcgg cctcgcgcca gtggcggacg
     2101 agcgggccgc ctgcttcgcg gtcgcgctgg cgttcatcgc ctggaacctc ggcccgtggg
     2161 agagccacgc gcaggagctg ctcgaggggg tgttgtactc gctgctgctg gcggccggcg
     2221 tccgcctcat gcgcgcgcgt cgagatcatg gcgtcgcgcg ggcgggctgc tcgtcgacgc
     2281 cgacgagcgc tcgccgcacc agacctcgcg cttgacatgg ccgcgcgtgg cggcgatcgt
     2341 cgcggccgct ggcatgaccg ttctcgtgcg cctcgcctac gacggcaccg acttccacgg
     2401 ctacgcgcgc cagctcgcca ggcccgggca cccggagatc cgcacggtgc agggcgtgct
     2461 cgaggcggcg ctggtcgagg tctaccgcca gccggtggtc acgcgcgcgg cgagccgcac
     2521 ggacgcgggc gtgcacgcgc tcgggcaggt cgtcggctgg gagccgccgc acccgatccc
     2581 cgcgcgcggg ctcatgctcg gcctgctcgc cagggtcccg cgcgacgtcg gcgtgctcgc
     2641 ggtctgggag gccgcgcttc ccgacggcac cccggtcgac ccgcgctttt ggaacctcgg
     2701 caagcgctac cgctaccgca tccgcaccac cccgcgccgc gtcccccaga ccgcccgcgg
     2761 cgagtggcac ctcccgcgcg tgctcgacgt cgcggcgatg caggccgccg ccgggcactt
     2821 cgccggcgag cacgacttcg cgggctttcg cgcgagcgcc tgccagtcgc gcacgaccgt
     2881 gcgccggctg cacgcggtga ccgtcaccgg ccggccctcc ggcgacgagc tgccggacga
     2941 cccccgttgt gagcccgacg acgggcgcgc cgaggtgatc gagatcgacg tccgcggcga
     3001 ggccttcctg cagaacatgg tgcgcatcat cgtcggcacg ctcgtcgagg tcggcctcgg
     3061 ccgccgcgcc cccgactcga tcgccgcgct gctgcagcac cccgaccgcc gccggtccgg
     3121 cgccaccgcc ccgccgcagg ggctgacgct cgtcgaggtg ctgtggccct cgacctggcc
     3181 accagccgac ggcgtcgccg cgctcgacct ctgacccgct ctccgtctca tcggcaacga
     3241 aacacgtgca cctcgccgtc gtggtcgagc gtccacagga ccccgcgcag gtgcaggtcg
     3301 agggcgaggt ccgtcagccg cgtcgacgca ccttccatct gctcccgcgg acatgtcccc
     3361 agggccatgc gggcgaccgc ggcgaggtcc gggaaggcgc cgaggtactc gccgtggcag
     3421 gcgtgacccg ccagcgcggc gggcaaccgc gggctgcgcg tacgctgggg tcgctcgccg
     3481 cgacggacga tgcgggcgtc gttgaggcgg cgagcctgcg cgcgcgcccc agcgaccgcg
     3541 tcgtcgagcg cgtcgagcac ggccggcagc tcgcgcggag caccgggcgc cagcaccgga
     3601 cgcagcagcg ccagcaggcg ccggcgcagc gccagcaaca cgacccgctc gtcgcggtcg
     3661 atctcgcggg cgacgtgact ggcgcgcacg ggcggggagg caggggcagg gcggggcaca
     3721 gcgccgaggt cgtccgccgc ccccgcgccg ttgcgcgggc gcgccgagaa aatcgcggca
     3781 cccggtcgcg ggtctgcgct accctgcccc gccatgtccc tcccgcaacg tccccgcgca
     3841 gtgatcaccg gcgcaggtgg tggccttggt cgcgccctga gcctcgagct cgcccgtcgc
     3901 cgcggccgcg tgctcgcggc cgacatcgac ctcgacgccg ccgagatcac cgcggccgcg
     3961 gtgcgtgacg ccggcggcga ggcggtggcg gtcgcctgcg acgtgtccca gcaggcccag
     4021 gtcgaggcgc tacccggcca ggccgacgcg gccttcggcg gcaccgacct tatcatcaac
     4081 aacgccggcg tcgccgcggc gggcccgatg gccgcgatcc ccgtcgccga ctg
//
LOCUS       DASWNJ010000848         1870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_474390, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000848 DASWNJ010000000
VERSION     DASWNJ010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474390"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VGB85_14420"
     CDS             complement(<1..549)
                     /locus_tag="VGB85_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135275.1"
                     /translation="MPLTREQLQELSRAFPKRGASRHDPTGYETLRTAIIEAIRPFAD
                     GLEFELDTVEPLTQAELLELPAHILLVWCEVDELTAYLYAIPEAELNEGVAQALVMIE
                     GCSFKDAGQLSPEQWGAALRLMAAVATPYARDAPDFFGAFVALEGAPQDALPDLAEVE
                     ALFDSFAPCFVEGGVGLSHRFSR"
     gene            complement(638..>1870)
                     /locus_tag="VGB85_14425"
     CDS             complement(638..>1870)
                     /locus_tag="VGB85_14425"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY0135276.1"
                     /translation="GTGSFVSVSNVDVVGEPRPAAAYDGSISASVPLLHEHLPTARVQ
                     RSKILVIDDHPEIVRLISRLLAPLHDIITASNGEEGLQKVHDEKPDLVVSDVMMPKMS
                     GFQLVERMRAEPATARLPVLLLTARADGHDRVRGLRRGASDYLVKPFLPEELKARVDN
                     LLRHQHYESYMTRLNEDLEASKNSLEGRVHGLFVDTVRTLVAAIDAKDTYTGGHSERV
                     SFFSVRIAEQLKLPRPMVRTIELGALLHDVGKIGIPDKVLNKPGALTLDEIEVIRQHT
                     IFGGQILEKSPELAELRRFALHHHERWDGTGYPDRLAAVDIPQSVRVVSVADCWDAMV
                     SDRIYRAGMEPQVAAEKVAKLGGSQFDPEVVEAMMAVWRQLELPPYLRPLKSRMVPHE
                     NRSAPRVSECGETYHVHD"
BASE COUNT          263 a          573 c          747 g          287 t
ORIGIN      
        1 gcgggagaag cggtggctga ggccgacgcc gccctcgacg aagcaggggg cgaacgagtc
       61 gaacagggcc tcgacctcgg cgaggtcggg gagggcgtcc tgcggggcgc cctcgagcgc
      121 cacgaaggcg ccgaagaagt cgggggcgtc ccgcgcgtag ggggtggcga cggcggccat
      181 caggcgcagc gcggcgcccc actgctcggg cgagagctgg ccggcgtcct tgaagctgca
      241 accttcgatc atcaccagcg cctgcgcgac gccctcgttg agctcggcct cggggatcgc
      301 gtagagatag gcggtcagct cgtcgacctc gcaccagacc aggaggatgt gcgccggtag
      361 ctcgagcagc tcggcctgcg tcagcggctc gacggtgtcg agctcgaact cgaggccatc
      421 ggcgaacggg cgaatggcct cgatgatcgc ggtgcgcagc gtctcgtagc cggtggggtc
      481 gtggcgcgag gcgccgcgct tggggaacgc ccgggacagt tcttgcagtt gctcgcgcgt
      541 cagtggcatc ggtcacctcg gggtgcggcg gcggccagca taacgcggac ctgcgccgcc
      601 ggggtgctcc ggacagggcg cacgatcgcg cgaggactca gtcgtgcacg tggtaggtct
      661 cgccgcactc gctgacgcgc ggggcggagc ggttctcgtg cgggaccatg cgcgacttga
      721 gcgggcgcag atacggcggg agctcgagct ggcgccacac ggccatcatc gcctcgacga
      781 cctcggggtc gaactggctg ccgccgagct tggcgacctt ctcggcggcg acctggggct
      841 ccatgccggc gcgatagatg cggtcggaga ccatggcgtc ccagcagtcg gcgacgctga
      901 cgacgcggac gctctggggg atgtcgacgg cggcgaggcg gtcggggtaa ccggtgccgt
      961 cccagcgctc gtggtgatgc agggcgaagc ggcgcagctc ggcgagctcg ggcgattttt
     1021 ccaggatctg gccgccgaag atcgtgtgct ggcgaatgac ctcgatctcg tcgagggtca
     1081 gggcgccggg cttgttgagg accttgtcgg ggatgccgat cttgccgacg tcgtgcagca
     1141 gcgccccgag ctcgatcgtg cggaccatcg gccgcgggag cttgagctgc tcggcgatcc
     1201 ggaccgagaa gaagctgacg cgctcggagt ggccgccggt gtaggtgtcc ttggcgtcga
     1261 tcgcggcgac gagcgtgcgc acggtgtcga cgaacaggcc gtgcacgcgg ccctcgaggc
     1321 tgttcttgct cgcctcgagg tcctcgttga ggcgggtcat gtagctctcg tagtgctggt
     1381 ggcgcagcag gttgtcgacg cgggccttga gctcctcggg caagaagggc ttgacgaggt
     1441 agtcgctggc gccgcgtcgc aggccgcgga cgcggtcgtg gccgtcggcg cgggcggtca
     1501 gcagcagcac ggggaggcgg gcggtcgccg gctcggcgcg catgcgctcg acgagctgga
     1561 agccgctcat cttgggcatc atgacgtcgg agacgacgag gtcgggcttc tcgtcgtgga
     1621 ccttttgcag gccctcctcg ccgttgctgg cggtgatgat gtcgtgcagc ggcgcgagca
     1681 ggcgggagat caggcggacg atctccgggt ggtcgtcgat gacgaggatc ttcgagcgct
     1741 gcacgcgggc ggtcgggagg tgctcgtgca gcagcgggac cgaggcggag atcgacccgt
     1801 cgtaggcggc cgcggggcgc ggctcgccga cgacatcgac gttggagacg ctgacgaagc
     1861 tgccggtgcc
//
LOCUS       DASWNJ010000849         2473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_474406, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000849 DASWNJ010000000
VERSION     DASWNJ010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474406"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..1028
                     /locus_tag="VGB85_14430"
     CDS             33..1028
                     /locus_tag="VGB85_14430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0135277.1"
                     /translation="MLLSRRRISTALLTGLLIAACGGGSGEATLRLGYFPNITHAAAL
                     VGVDDGIFARQLGATKLEPFTFNAGPAAIEALLSGSLDASYVGPNPAINAYVKSRGDA
                     VRIVAGATSGGAFLVVHADIIGPADLRGKQIASPQLGGTQDVALRTWLGEQGLKTDTS
                     GGGDVSVVPQENAQTLERFREGKLAGAWVPEPWASRLVLEGGGKVLVDERDLWPNKQY
                     TTTVLLVRRDYLQQHPDRVEALLRGHVEATKWLQANAEDAKTHVNAAIEKLTGKPIGK
                     PVMDRAFPSLTFTTDPQMASLEKSAADAEKLGLLKLGGVNLAQLHDPALLQKLAP"
     gene            complement(1035..>2473)
                     /gene="dbpA"
                     /locus_tag="VGB85_14435"
     CDS             complement(1035..>2473)
                     /gene="dbpA"
                     /locus_tag="VGB85_14435"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:NF008744.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HEY0135278.1"
                     /translation="DRTPVPRMTETPPTPPDPSLPSGPGSFATLSLAAPLARAIEVLG
                     FSQMTPVQAHALPDLLRGKDVIAQARTGSGKTVAFGLALLAGVDVELARVQALVLCPT
                     RELADQVSAEIRRLARFVANLRVVTLCGGVPVRTQKPALQVPPHVVVGTPGRILDHLA
                     RENLALADLRVLVLDEADRMLDMGFLDAITEVVEQAPKARQTLLFSATYPDDIRALSR
                     RLQRDPSTVTVDTVVGADIEQTFFAVEPARKLDALAALLLYHRPESALVFCHTRNDVR
                     DVSARLAERGFSVLALHGELLQRERDEVLLRFVNHSCTVLVATDVAARGLDIKGLPAV
                     IAWELPNDPDVHLHRIGRTGRAGLRGQAFALCAPGERERAHALERTQGSKIRWGTLPV
                     PQDSRPMRPPMTTLLLEGGRQDKLRPGDLLGALTGDMGLAADVIGKIDIHARHSYVAV
                     TAGQAERALQGLRAGKIKGRSFRARVLR"
BASE COUNT          367 a          865 c          901 g          340 t
ORIGIN      
        1 gaaggatatt tcagaatggt atacgaaaac gcatgctgct gtctcgccgc cggatctcca
       61 ccgcgctcct gactggcctg ttgatcgccg cctgcggggg tggcagcggc gaggcgacgc
      121 tgcggctcgg ctacttcccg aacatcacgc acgccgcggc gctcgtcggc gtcgacgatg
      181 ggatcttcgc ccgccagctc ggcgcgacca agctcgagcc cttcaccttc aatgccgggc
      241 cggcggcgat cgaggcgctg ctctcgggct cgctcgacgc cagctacgtc gggccgaacc
      301 cggcgatcaa cgcgtatgtc aagtcgcgcg gcgatgccgt gcgcatcgtc gccggcgcca
      361 cttccggcgg cgccttcctg gtcgtgcacg ccgacatcat cggtccggcc gatctgcgcg
      421 gtaagcagat cgcctcgccg cagctcggcg gcacccagga cgtcgcgctg cgcacctggc
      481 tgggcgagca gggcctcaag accgacacct ccggcggcgg cgacgtcagc gtcgtcccgc
      541 aggagaacgc gcagacgctc gagcgcttcc gcgagggcaa gctcgcgggc gcctgggtgc
      601 ccgagccgtg ggcctcgcgg ctggtgctcg aggggggcgg caaggtcctg gtcgacgagc
      661 gcgacctgtg gccgaacaag caatacacga cgacggtgct gctggtccgc cgcgattatc
      721 tgcagcagca cccggaccgc gtcgaggcgc tgctgcgcgg gcacgtcgag gcgacgaagt
      781 ggttgcaggc caacgccgag gacgccaaga cccacgtcaa cgcggcgatc gagaagctga
      841 ccggcaagcc gatcggcaag ccggtgatgg accgcgcctt cccgagcctg acctttacca
      901 ccgacccgca gatggcctcg ctcgagaagt cggccgccga cgcggagaag ctcggcctgc
      961 tcaagctcgg gggcgtgaac ctggcccagc tgcacgaccc ggccctgctg cagaagctcg
     1021 cgccctgagg ccgtttagcg caggacccgg gcgcggaacg agcggccctt gatcttgccg
     1081 gcgcgcaggc cctggagggc gcgctcggcc tgcccggcgg tgacggcgac gtagctatgg
     1141 cgcgcgtgga tgtcgatctt gccgatcaca tccgcggcta aacccatgtc cccggtcagc
     1201 gcgccgagca ggtcacccgg gcgcagcttg tcctgccggc cgccctcgag cagcagcgtc
     1261 gtcatcggcg ggcgcatcgg ccggctgtcc tgggggacag gtagcgtgcc ccagcggatc
     1321 ttgctgccct gcgtgcgctc gagcgcgtgg gcccgctcgc gctcgcccgg ggcgcacagc
     1381 gcgaaggcct ggccacgcag gcccgcgcgg ccggtgcgac cgatccggtg caggtgcacg
     1441 tcggggtcgt tcggcagctc ccacgcgatc acggccggca gtcccttgat gtcgaggccg
     1501 cgagcggcga cgtcggtggc gaccagcacc gtgcagctgt gattgacgaa gcgcagcagc
     1561 acctcgtcgc gctcgcgctg caagagctcg ccgtgcagcg ccagcaccga gaacccgcgc
     1621 tcggccagcc gcgcgctgac gtcgcggaca tcgttgcgcg tgtgacaaaa gaccagcgcc
     1681 gactcgggcc ggtggtacag cagcagggcc gcgagcgcgt cgagcttgcg cgcgggttcg
     1741 acggcaaaaa aggtctgctc gatgtcggcg ccgacgaccg tgtcgacggt gacggtgctc
     1801 gggtcgcgct gcagcctgcg gctcagcgcg cggatgtcgt ccgggtaggt cgcggagaac
     1861 agcagggtct ggcgggcctt tggcgcctgt tccacgacct cggtgatcgc gtcgagaaag
     1921 cccatgtcga gcatgcggtc ggcctcgtcg agcacgagca cgcgcaggtc cgcgagcgcc
     1981 aggttctcgc gcgcgaggtg atcgaggatc ctccctggcg tgccgacgac gacgtgcggc
     2041 gggacctgca gcgccggctt ctgcgtgcgc accggcaccc cgccgcacag cgtcacgacc
     2101 cgcaggttgg cgacgaagcg cgcgagccgg cggatctccg cgctgacctg gtcggcgagc
     2161 tcacgcgtcg ggcacagcac gagggcctgc actcgcgcca gctcgacgtc gacgccggcc
     2221 agcagcgcca gaccgaaggc gaccgtcttg ccggagccgg tgcgcgcctg ggcgatcaca
     2281 tccttgccgc gtagcaggtc cggcagcgcg tgggcctgca ccggcgtcat ctgcgagaag
     2341 ccgagcacct cgatcgcgcg ggccagcggc gcagcgagcg agagcgtggc aaagctcccc
     2401 ggtccggagg gcagggaggg atccggaggt gtcggcggcg tctcggtcat gcgtgggacg
     2461 ggggtgcgat cgt
//
LOCUS       DASWNJ010000850         4280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_368057, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000850 DASWNJ010000000
VERSION     DASWNJ010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368057"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1991
                     /locus_tag="VGB85_14440"
     CDS             <1..1991
                     /locus_tag="VGB85_14440"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEY0135279.1"
                     /translation="IVWTRVEDSELGEDDAELRLELATDAEFTSPVLLDGEDHVLVPA
                     RAMFDRCAKAKVRGLSAATTYYYRFIHTRADGRFVSPVGRTRTAPAADADAKLRFAVV
                     SCQDFNGRYYNGYAHMAAMELDFIVHLGDYIYETTGDPTFQTTGGRSAVFTDKAGAIV
                     FNAGTEDEYFAAKSLDNYRELYRIYRSDPALRKIHETVPMICTWDDHEFSDDAHGANA
                     TYFDGRQDEHDPQRRRNANQAWFEYMPVDFQEEDFEYDPAKEFPGDIQIYRDFGFGKH
                     LRLVMTDLRSYRSDHLIAEDAFPGAVVLTQAELMAELGELPADASEYIDIDAFAGGTY
                     KTALIAGAAQLDYDAAKISGKLDVTYVNSLLAKLGDPVAQIVELVGLERGYAYRHLGK
                     ISPHASIGARYLVAKDAFDLWARINYKKTQGKSQDVMGAAQQAWFLDTMKGAKETWKL
                     WGNEYCLLPLAINLKGLAPAPFDKAFYMNADMWDGMRDRRSEILTELAKLDNVVAITG
                     DIHAFYAGVPGTNEDASQRIVEFVTSGMSSGTFRAQLVSQVAADPVLSKTDGASMLAE
                     SIDSLLRDTGFNRHLAHADSSRNGFVTIELDGAETVASYHAIAEDEVFTDHEAMAAAE
                     LAAKFETKRFKVEAGKRELFMEIAGAWKRWDSETNRFV"
     gene            complement(2060..3070)
                     /locus_tag="VGB85_14445"
     CDS             complement(2060..3070)
                     /locus_tag="VGB85_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135280.1"
                     /translation="MLRPMWNLSLMTVLTATALVACGDDKGTSDTDVASTGGATEPAT
                     MTDPTASSTDEPTAGTTGSATEDPTPTTSTDPTMGGDTAADGDPCTANGDCASQGCLK
                     YRDLEAGQCAAAPAGGNTRIAGTLVDFATNAPVPGAELRVIGALSALSDPANAPPVIT
                     ATADASGQVDVVSATPISEGIGIVGIITGGDYYTSATGLASPTAGVYGPMNGNRDIWA
                     VPAAKLTEWSGFLMADADIATSLPLGEKGGVVGFVRDGAGTGKAGAKVVSVKGADSTA
                     KIRYLAEDGMSFTSDMTSSNGIFVLVGPGLAEKFKVEGGTVEGTAGSAEKAVFVMILT
                     EP"
     gene            complement(3249..3968)
                     /locus_tag="VGB85_14450"
     CDS             complement(3249..3968)
                     /locus_tag="VGB85_14450"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEY0135281.1"
                     /translation="MATFTICAFSLGLFCSASDMQAAPAAAPSAPVAAPETAGDKVSA
                     SAVLASVQSFYDGTVDLLGTFRQTYVHQVYGTRTVSHGKLQLRKPGMMVWDYDGAADP
                     DFYVDGQKLWVVEHDTRQVVSQKVDGNTNLAGAMKFLFGGQKLIKDFKVRMAPEKPAK
                     SYGMPGHKVIELKPKEQNPHYKTLLLVVDDATGRVDAFVVRSQDNSTNHFVLTDVTTN
                     KGLDAKDFKYKVPKGYVEAKQ"
BASE COUNT          694 a         1493 c         1428 g          665 t
ORIGIN      
        1 tgatcgtgtg gacgcgcgtc gaggactccg agctcggtga ggacgacgcc gagctgcgcc
       61 tcgagctcgc caccgacgcc gagttcacga gcccggtgct gctcgacggc gaggaccacg
      121 tgctggtgcc cgcgcgcgcc atgttcgacc gctgcgccaa ggccaaggtc cgcgggctgt
      181 cggccgcgac cacctactac taccgcttca tccacacgcg cgccgacggc cgcttcgtga
      241 gcccggtcgg ccgcacgcgc acggccccgg cggcggacgc cgacgccaag ctgcgcttcg
      301 cggtcgtctc ctgtcaggac ttcaacggcc gctactacaa cggctacgcg cacatggccg
      361 cgatggagct cgacttcatc gtgcacctcg gcgactacat ctacgagacg accggcgacc
      421 cgaccttcca gacgaccggg ggtcgctccg ccgtgttcac cgacaaggcc ggcgcgatcg
      481 tcttcaacgc gggcaccgag gacgagtact tcgccgcgaa gagcctcgac aactaccgcg
      541 agctgtaccg catctaccgc tcggaccccg cgctgcgaaa gatccatgag accgtgccga
      601 tgatctgcac ctgggacgac cacgagttca gcgacgacgc ccacggcgcc aacgcgacct
      661 acttcgacgg ccgccaggac gagcacgacc cgcagcggcg caggaacgcc aaccaggcgt
      721 ggttcgagta catgccggtc gacttccagg aggaggattt tgaatacgac cccgcgaagg
      781 agttccccgg cgacatccag atctaccgcg acttcggctt cggcaagcac ctgcgcctgg
      841 tgatgaccga cctgcgaagc taccgcagcg accacctgat cgccgaggac gcgttccccg
      901 gcgcggtcgt gctgacgcag gccgagctga tggccgagct gggagagctg cccgcggacg
      961 ccagcgagta catcgacatc gacgccttcg ccggcggcac ttataaaacg gccctgatcg
     1021 ccggcgcggc gcagctcgac tacgacgccg ccaagatctc cggcaagctc gacgtcacct
     1081 acgtcaacag cctgctcgcc aagctcggcg atccggtcgc ccagatcgtc gagctcgtcg
     1141 gcctcgagcg cggctacgcc taccgccatt taggaaagat cagcccgcac gcgtcgatcg
     1201 gcgcgcgcta cctcgtcgcc aaggacgcct tcgacctgtg ggcccggatc aattacaaaa
     1261 agacgcaggg caagagccag gacgtcatgg gcgcggccca gcaggcgtgg ttcctcgaca
     1321 ccatgaaggg cgcgaaggag acctggaaat tgtggggcaa cgagtactgc ctgctgccgc
     1381 tggcgatcaa cctcaagggc ctcgcgcccg cgcccttcga caaggccttc tacatgaacg
     1441 cggacatgtg ggacggcatg cgtgaccgcc ggtcggagat cctcacggag ctggcgaagc
     1501 tcgacaacgt cgtcgcgatc acgggggaca tccacgcgtt ctacgccggc gtgcccggca
     1561 ccaacgagga cgcgagccag cgcatcgtcg agttcgtcac cagcggcatg agctccggca
     1621 cgttccgcgc ccagctggtc tcgcaggtcg ccgccgaccc ggtgctcagc aagaccgacg
     1681 gcgcctcgat gctggccgag agcatcgact cgctgctgcg cgacaccggc ttcaaccgcc
     1741 acctcgcgca cgccgactcg tcgcgcaacg gctttgtgac gatcgagctc gacggcgccg
     1801 agacggtcgc ctcctaccac gcgatcgcag aggacgaggt gttcaccgac cacgaggcga
     1861 tggccgccgc cgagctcgcc gccaagttcg agaccaaacg cttcaaggtc gaggcaggca
     1921 agcgcgagct gttcatggag atcgcaggcg cgtggaagcg ctgggactcc gagacgaacc
     1981 gcttcgtgtg aatttttaaa agaaaagaga aaagcgcgac cagggggacc tggtcgcgct
     2041 tctgcgttca cgcgagcgct cagggctcgg tcaggatcat cacgaacacg gccttctcgg
     2101 cgctgcccgc ggtgccctcg acggtgccgc cctcgacctt gaacttctcg gccaggccgg
     2161 ggccgacgag cacgaagatg ccgttcgacg aggtcatgtc gctggtgaag ctcatgccgt
     2221 cctcggcgag gtagcggatc ttcgccgtgc tgtcggcgcc cttgacggag acgaccttgg
     2281 cgccggcctt gccggtgccc gcgccgtcgc gcacgaagcc gaccacgccg cccttttcgc
     2341 cgagcggcag cgaggtggcg atgtcggcgt cggccatcag gaagccgctc cactcggtca
     2401 gcttggcggc gggcacggcc cagatgtcgc ggttgccgtt catcggcccg tacacgcccg
     2461 ccgtcggcga ggcgaggccg gtggccgagg tgtagtagtc gccgccggtg atgatgccga
     2521 cgatgccgat gccctcggag atcggtgtgg ccgacacgac gtcgacctgg cccgaggcgt
     2581 ccgcggtcgc ggtgatcacc ggcggcgcgt tggccgggtc gctcagcgcg ctcagggcac
     2641 cgatcacccg cagctcggcg cccggcaccg gcgcattggt ggcgaagtcg accagcgtgc
     2701 cggcgatccg cgtgttgccg cccgccggag cggccgcaca ctgaccagcc tcgaggtcgc
     2761 ggtacttgag gcaaccctgc gaggcgcagt cgccgttggc cgtgcaggga tcgccgtccg
     2821 ccgccgtatc cccgcccatc gtcgggtcgg tcgaggtcgt cggcgtcggg tcctccgtgg
     2881 cgctccccgt cgtgcccgcc gtcggctcgt ccgtcgacga ggccgtcggg tccgtcatcg
     2941 tcgccggctc ggtcgccccg cccgtcgagg ccacgtcggt gtcggaggtc cccttgtcat
     3001 cgccgcacgc caccagggca gtcgcggtca acacggtcat caggctcaag ttccacatcg
     3061 gtcgaagcat caggttgtct cccaaaaaag gctgcaacgc cggcaggcgt cgccgcgcga
     3121 cggtaccagg ccgccgcgcc accgccgcat gcgagccacg cgtggaccct cacacgccgc
     3181 tcgcgagcgg gcccgaccac cgcattcgcg gggccgggcc ttgggtgatc acccgtgcga
     3241 acgagcgttc actgcttggc ttcgacgtag cccttgggga ccttgtactt gaagtccttg
     3301 gcgtcgaggc ccttgttggt ggtgacgtcg gtcagcacga agtggttggt gctgttgtcc
     3361 tggctgcgca cgacgaaggc gtcgacgcgg ccggtcgcgt catcgacgac cagcagcagg
     3421 gtcttgtagt gcgggttctg ctccttgggc ttgagctcga tgaccttgtg gccgggcatg
     3481 ccgtagctct tggccggctt ctccggggcc atgcgcacct tgaagtcttt gatcagcttt
     3541 tgcccgccga acaggaactt catcgcgccc gcgaggttgg tgttgccgtc gaccttctgg
     3601 ctgacgacct gacgcgtgtc gtgctcgacg acccacagct tctgaccgtc gacgtaaaag
     3661 tcggggtcgg ccgcgccgtc gtagtcccac accatcatgc cgggcttgcg cagctgcagc
     3721 ttgccgtggc tgaccgtgcg cgtgccgtag acctgatgga cataggtctg acggaaggtg
     3781 ccgagcaggt cgaccgtgcc gtcgtagaag ctctgcacgc tggcgagcac cgcggaggcc
     3841 gagaccttgt caccggcggt ctcgggcgcc gcgaccgggg ccgacggcgc tgcggcgggc
     3901 gcggcctgca tgtcggacgc cgagcagaaa agaccgagag agaaggcgca gatcgtgaag
     3961 gttgccatga taaagctccg agctcctgtg ggtcgcccca cctggggtcg acagacgccg
     4021 gaccccggag ggcgattcac ggcgtccgta aacgcagtgt aaacccgccc cctggcgtcc
     4081 gggcgggcct gtggcctcgt ctagcacggt tttgccggcg cggcgagccg cgcgcagtct
     4141 gcgtcaccgg cggcccggga ccggcctcag gcggcctgca cgaagacctc gcgcggcgca
     4201 gtgccacgcg ccgggccgat caccccctcg gcctccatct gctcgacgat cttggcggcg
     4261 cggttgtagc cgatgttgag
//
LOCUS       DASWNJ010000851         5073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_121113, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000851 DASWNJ010000000
VERSION     DASWNJ010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121113"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1100..2638
                     /locus_tag="VGB85_14455"
     CDS             1100..2638
                     /locus_tag="VGB85_14455"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEY0135282.1"
                     /translation="MVVKRTRVANPEAAAAELAGHYARILGQHADRQVSNVIRTCLRR
                     LPSCLPIQGEDLRAVEELIERIIRLGEVGARDPIGSPREIDDYIARRLGEPASGARRV
                     YRALRAMLGHTVLVERRSRQIWVLRLSGLTDSSSILHRTLLAETPALYSLPASDTDSA
                     AGSEAPCSATADDPVTPSQPPEPLEDPSEPLRAELARLHEELAAERLARVEVDKRREA
                     TDAALGEEKRARSEADQRAQVAAAQLTVLERELDTLRRALDQERRARGEADLRGQANA
                     AQCTTLVQELGLLRGALDEERRARGDADLRVQFAEAELVVLGEAQAADAATVRRLIEC
                     DQVLGAAAFLIARMHGHSVEVALQSLHQLLPAAAAVLSASTDQSPQAAPNGGTPGVPA
                     ATDSIPPSQAVPPTQGPLRPTSGAPTVSPHSTLAAPLPSSLPPAPASSDRSAAIQAPD
                     PVPAASSRRPPTQRLRASQAPARLAPGMESDQPNVKPSKIGRNDLCPCGSGAKFKRCC
                     GRPA"
     gene            2746..3180
                     /locus_tag="VGB85_14460"
     CDS             2746..3180
                     /locus_tag="VGB85_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135283.1"
                     /translation="MCILACAYTPTRPLPTLAATRNPPATAPAPSNPGPPVAPLDLCP
                     DADETLNDYRDDDGCPDEQPIQKTNGDPNSGSSTKINACGHADHWNNWNWNEKTGVPA
                     SRGILPEGTGVEWRYITIDGAGAMVMLAKDDWVFVPSDCLSV"
     gene            complement(3190..>5073)
                     /locus_tag="VGB85_14465"
     CDS             complement(3190..>5073)
                     /locus_tag="VGB85_14465"
                     /inference="COORDINATES: protein motif:HMM:NF016358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF499 domain-containing protein"
                     /protein_id="HEY0135284.1"
                     /translation="VCRAFADLYADNSDKFPAETRDAAYLRRLKASYPIHPEVFERLY
                     EDWSTLPKFQRTRGVLRLMARMVHSLWRSDNRDYLILPGSIPLDDHSVRNELIKYLPS
                     GWDPIIDKDIDGTNAEPRRIDDDISTLGSIQACRRVARAIFLGSAPSVGTQKVRGVGV
                     ERVRLGCVQPGQSPGKYDDALKRLSDRLHNLYSGNERFWFDLRANLRREMEDRLQRFD
                     LKLHVYPEIRDRLQKLVKSSTVQAVHVFTGSKDIPDKPDLRLVVLDPDHGHKRKDENS
                     KAVLGATAILKNHGDKPREYQNRLLFLVPELNAVQPLRDSVQRYLAWKSIVDDADELN
                     LDKHQGKEVAASVSDAANRVDGSLREAYRILLAPMAEVDDVSNIVWEDEALTLAGSSY
                     DKAIASTTREREWVISAWAPTHLAVLLAKWFWKDDKPDVGTKKVWLDTCRYLYLPRLA
                     TEEVFASTVRDGIKRREWFGYAAGAKDGGYSGLVLGSFGIVYIDDSSLLIHPDTARLA
                     AVKPTGGDGNEILEPDFKLTGDDPPTKDTISPPGGGGVSPPKFRRFHATVDVGVTDPI
                     GSFTDIFENVLQHFTAQYGTRVSVTLDIEAALPAGFDDRTVRVVKENATTMKFKIAEF
                     ETE"
BASE COUNT          800 a         1714 c         1711 g          848 t
ORIGIN      
        1 ggcgcagtgg gtctccatcg tggtgcgcgt ggcgctgtcg aggtcctgcg gggtgcggag
       61 caagacggca ccgagggtct ccgcgaggtt gcgggcgagg gtggattttc cacaaccgtc
      121 gagaccctcg acggcgatga atagtgggtt gtgcggcatg atggcaaatt ccaggcgggt
      181 gggctcaggc gggtggcggg gcatgaaaag tggcggacgg cggccctcac cgagggctgt
      241 tggggccggg ctcgccggat tttggcgctc gaccttcagg ggcgccttgt cctggccggc
      301 ggcaggatca gggtccgatg cgacactctc accgagatcg cggccatgat cgcggcgcgc
      361 tggggcacct gctgcgggtg ccggtcctcg ccgaccgtgc tcccggcgat cctcggtctt
      421 gccggccggg gtcgtcggcc aggacgaagc ggagcggctg cttcggcaac cttgactcgt
      481 tgaccgccgc gatctggcgc ggcggcggat ccacgcgagg tgtggatcgc caccatcaga
      541 tcgaagaggt tcacgtctcc gtggcggccg tggccgcgcg atgcgagctg cccacagtgc
      601 ggcgccgcgc cagccttaat ctgtgcccgc acgagacccg ccacacccct tgagcgacgg
      661 cggcctgccg cgcgcgctcc gttgctgtac tccaggagca cccggggcgg gctttatggt
      721 gtgcgggccg ccgagctcgc taccagtcag gcgcgactgc acgtcgagtg cacgcaagtc
      781 gtgctcgtct gcgtatcgag agagtgcgga cttgtgctca tatgcccttc gagtggggca
      841 gaggtcgccc caagatcgat ccgaccaccg gccaagtagc gatcgagtgt acggcgagtc
      901 ggcgccaagt aggtcacgat gtggcccgat gtggcccgat gcgggcccga tgtgggcccg
      961 atacgggcac gatacgggca cgatgagtgc ggtatcggct cccggtattg gcgggatatt
     1021 ggcacgcgcg agattcgcta tccaccctca cggtgtcctc ggatagtctc tggcggggta
     1081 ttcggggagg gagaggcgga tggtggtcaa gcgcactcgc gtggcgaacc cggaggcagc
     1141 agcggccgaa ctggcggggc actacgctcg gatcctcggg cagcacgcag accgacaggt
     1201 ctccaacgtc atccgcactt gcctgcgccg actcccatca tgtctcccga tccagggtga
     1261 ggacctccgt gccgtcgagg agttgatcga gcgcatcatc cggctcgggg aggtcggcgc
     1321 acgcgatccc atcggctcac cccgggagat cgacgactac atcgcgcgtc gactcgggga
     1381 gccggccagc ggcgcgcggc gcgtataccg tgcgctccgc gccatgctcg gtcatacggt
     1441 cctcgtcgaa cggcgcagcc gtcagatctg ggtgctgcgc ctgtccggcc tcacggactc
     1501 atcctcgata ctgcaccgga cgctgctggc cgagacgccg gcgctctact cgttgccagc
     1561 gtccgacacg gactcggccg ccgggtccga agccccatgc agcgcgaccg ctgacgatcc
     1621 cgtcaccccg tcgcagcccc cggagccgct cgaggatccc tcggagccgc tccgcgccga
     1681 actcgcccgg ctccacgagg agcttgccgc cgagcggctg gcgcgggtcg aggtggacaa
     1741 gcgccgagag gccaccgacg ccgctctcgg cgaggaaaag cgcgcgcgca gcgaggcgga
     1801 ccagcgggct caggtcgccg cagcccagct caccgtgctc gagcgggagc tcgacacgct
     1861 gcggcgcgcc ctcgaccagg aacgccgagc acgtggcgag gctgaccttc gcggccaggc
     1921 caacgcagcc cagtgcacca cgctcgtgca ggagctgggc ctgctgcgcg gcgcactcga
     1981 cgaggaacgc cgcgcacgtg gcgacgctga cctgcgcgtc caattcgcag aggccgagct
     2041 cgtcgttcta ggtgaggcgc aggctgcgga cgcagccacc gtgcggcggc tgatcgagtg
     2101 cgatcaggtg cttggggcgg ctgccttcct catcgcccgc atgcacggtc acagcgtcga
     2161 ggtcgcgctt cagtccctcc accagctctt gccggccgca gccgccgtgc tctcggcgtc
     2221 gacggaccag tcgccgcagg ccgcccctaa cggcggcacg ccaggggtcc ccgctgctac
     2281 tgacagcata ccgccatccc aggctgtccc acccacgcag gggccactcc gacctacgag
     2341 cggcgcgccg acggtcagcc cccacagcac gctcgctgcg ccgctgccat cgtccctgcc
     2401 gccggccccg gcgtcgtccg accgctcggc cgcgattcag gcgccagacc cagtacctgc
     2461 ggcatcatcg cgcaggcccc cgacgcagcg gctacgcgcg agccaggcgc cggcccgact
     2521 cgcgcccgga atggagtccg accagcccaa tgtgaagccg agcaagatcg ggcgaaacga
     2581 tctttgcccg tgcggctcgg gcgcgaaatt caagcgatgc tgtggacgac ccgcgtagac
     2641 ggcgcggtgt agccggctgt ctcgcacgcc gcgatcctgc cgatcgatcc ccccgaggtc
     2701 ggtacttcag ccccgatgcc cctccgcacc cgaccctggc cgctcatgtg catactcgcc
     2761 tgcgcctaca cgcccacgcg cccgctacca acgctggcgg ccacccgcaa cccgccggca
     2821 actgcccccg cgccgtccaa cccgggccct ccagtcgccc ccctcgacct ctgccccgac
     2881 gccgacgaga ccctcaacga ctaccgcgac gacgacggct gccccgacga gcagccgatc
     2941 cagaagacga acggcgatcc aaacagtggt tcatccacga agatcaacgc gtgtgggcac
     3001 gctgatcact ggaataattg gaactggaac gagaagacag gtgtgccggc ctcccgtggc
     3061 attctcccgg aaggcacagg cgtcgagtgg cgctacatca cgatcgatgg cgccggcgcg
     3121 atggtgatgc tggcgaaaga cgactgggtg ttcgtgccca gcgactgtct gagcgtttag
     3181 acgcgatcgc tactccgtct cgaactcggc gatcttgaac ttcatggttg tggcattctc
     3241 cttgaccacg cgaaccgttc ggtcgtcgaa gccggctggc agcgcggctt cgatgtcgag
     3301 cgtcacggac acgcgcgttc cgtactgcgc agtaaagtgc tgtaggacat tctcgaagat
     3361 gtccgtgaag gagccgatcg ggtcggtaac cccgacgtcg accgtcgcat ggaagcgtcg
     3421 gaacttggga ggggatacgc cgcctccgcc tgggggggag atggtgtcct tcgtcggcgg
     3481 gtcgtccccc gtcagcttga agtcgggctc gaggatctcg ttcccatccc cacccgtcgg
     3541 cttcaccgct gccaagcggg ccgtatccgg atggatgagc agcgagctgt cgtcgatgta
     3601 gacgatgccg aagctgccga ggacgagccc cgagtagccg ccgtccttcg cgcccgctgc
     3661 atagccgaac cattcgcgcc gtttgatgcc gtcgcggacc gtgctggcga acacctcctc
     3721 ggtcgcaagc cgcgggaggt agaggtaacg gcaggtgtcg agccacacct tcttcgtgcc
     3781 cacgtccggc ttgtcgtcct tccagaacca cttcgccagg agcacggcga ggtgggtcgg
     3841 cgcccacgcc gagatgaccc actcccgttc gcgggtcgtg gacgcgattg ccttgtcgta
     3901 gctgctcccg gcgagggtga gcgcctcatc ctcccacacg atgttgctga catcatcgac
     3961 ttcggccatc ggcgcgagga ggatcctgta cgcctcgcgt aggctcccgt cgacgcggtt
     4021 ggccgcatcg ctgacgctgg ccgcgacttc cttcccctgg tgtttgtcga ggttgagctc
     4081 gtctgcgtcg tcgacgatcg acttccacgc gaggtaccgc tgcacactgt cgcgcagtgg
     4141 ctgcaccgcg ttgagctcgg ggacgaggaa caacagccgg ttctggtact cgcggggctt
     4201 gtcgccgtgg ttcttcaaga tcgccgtggc gccgagcacg gccttgctgt tctcgtcctt
     4261 tcgtttgtgc ccgtgatcgg ggtccagcac gacgaggcgg agatccggtt tgtccgggat
     4321 gtccttgctt ccggtgaaga cgtggaccgc ttgcacagtg ctgctcttta cgagcttttg
     4381 gagccggtcc cggatctcgg gatacacgtg gagcttcagg tcgaagcgtt ggaggcggtc
     4441 ctccatctcg cgccggaggt tcgcgcggag gtcgaaccag aagcgttcgt tgccgctgta
     4501 gaggttgtgg aggcgatcac tgagccgctt cagggcgtcg tcgtacttgc ccggtgactg
     4561 gcctggctgc acgcatccca gccggacgcg ctcgacgccg acgccacgga ccttctgcgt
     4621 accgacggaa ggcgccgacc cgaggaagat cgcacgagcg acgcgccggc acgcctggat
     4681 gctgccgagg gtggagatgt catcgtcgat ccggcgcggt tcagcgttcg tgccgtcgat
     4741 gtccttgtca atgatcgggt cccatcccga gggcaggtac ttgatcaact cgttgcggac
     4801 gctgtgatcg tcgagcggga tcgaacccgg caggatgagg tagtcgcggt tgtcgctacg
     4861 ccacaacgag tgaaccatcc gcgccatcag gcgcagtacg ccacgagtac gctgaaattt
     4921 cggcagcgtc gaccagtcct cgtagaggcg ctcgaagacc tcggggtgga tcgggtagct
     4981 cgcctttagc cggcgaaggt acgcggcatc acgtgtctcg gccgggaatt tgtcgctgtt
     5041 atcagcgtaa agatcggcga acgcgcggca gac
//
LOCUS       DASWNJ010000852         2576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_156791, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000852 DASWNJ010000000
VERSION     DASWNJ010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156791"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="VGB85_14470"
     CDS             <1..821
                     /locus_tag="VGB85_14470"
                     /inference="COORDINATES: protein motif:HMM:NF042462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MbnP family copper-binding protein"
                     /protein_id="HEY0135285.1"
                     /translation="LFVIASCDAAGDRAVALRFAPLVGGAPFACGQDFTGIGTGASTV
                     TPLDFRMYVHAVALVRAGGERVPLTVPDDGQWQRDGLVLLDFEDGSGACMTGSPSTNF
                     EVRGSAPDHDDYTGVELVVGVPHEMNHLDGATAPAPLNAQGLWWSWSGGYKYIRLDVR
                     PRTQPEFFYHLGATACDGSVKDGYDCAFANLAQIRIDGFDIDADELVIDAATVLAGVD
                     VERVPDGTTDMLPGCMAFPGDPECPAMMAPLGLRYESDEPGAQTLFTARPRAGK"
     gene            993..1322
                     /locus_tag="VGB85_14475"
     CDS             993..1322
                     /locus_tag="VGB85_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135286.1"
                     /translation="MACHQEHGPGPGRFTAAGTLYGADGTPHADGAIELRTGAAGTGD
                     LVRRIEADSNGNFYTTQALPLPDSALFPAVYDRSGALRNYMPFPTSSAACNVCHVGSA
                     VILIPDP"
     gene            1330..2448
                     /locus_tag="VGB85_14480"
     CDS             1330..2448
                     /locus_tag="VGB85_14480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbnH family di-heme enzyme"
                     /protein_id="HEY0135287.1"
                     /translation="MSIHRIALLLVAVSCTGSEPAAEPWVWELPAGFPEPHVPDDNPM
                     SVAKVELGRHLFYDKRLSHNETMACASCHEQARAFTDGKATTEGSTGHLLPRNAMSLT
                     NVGYLSVYTWANPELADLEEQALVPMFADYPLELGTQFNTPQILRRFADDPKYQQLFA
                     DAFPEDDDPVTTQRIVQAIASFERTLISGDAAYDRFIYGGDDAAMSASARRGMDLFFS
                     ERAECYHCHAGPNFSTSFRSRTSEAGPPDFQNNGLYNLDATGAYPPGNGGLFELTGEP
                     RDQGKFRVPTLRNVTLTAPYMHDGSIATLAEVIEHYDAGGREITGGPLAGDGRMNPNK
                     SPLVFPMELDEQEKADLVAFLESLTDPTFASDPRFAAP"
BASE COUNT          392 a         1000 c          816 g          368 t
ORIGIN      
        1 gtctctttgt catcgcctcg tgtgacgccg cgggcgaccg cgccgtcgcg ctgcgcttcg
       61 cgccgctcgt cggcggggcg ccgttcgcct gcggtcagga cttcaccggg atcggcaccg
      121 gcgcgtcgac cgtgacgccg ctcgactttc gcatgtacgt gcacgcggtc gcgctggtcc
      181 gcgccggtgg cgagcgggtc ccgctgaccg tgcccgacga cgggcagtgg cagcgcgacg
      241 gcctggtgct gctcgacttc gaggacggca gcggcgcgtg catgaccggc agcccgagca
      301 ccaacttcga ggtccgcggc agcgcgccgg accacgacga ttataccggc gtcgagctcg
      361 tcgtcggcgt cccgcacgag atgaaccacc tcgacggcgc gaccgctccg gcgccgctca
      421 acgcccaggg cctgtggtgg agctggtcgg gcggttacaa gtacatccgc ctcgacgtgc
      481 gcccgcggac ccagccggag ttcttttatc acctcggcgc gaccgcctgc gatggcagcg
      541 tcaaggacgg ctacgactgc gccttcgcca acctcgcgca gatccgcatc gacggcttcg
      601 acatcgacgc cgacgagctc gtgatcgacg cggcgaccgt gctcgcgggg gtcgacgtcg
      661 agcgggtccc ggacggtacg accgacatgc tgcccggctg catggccttc cccggcgacc
      721 ccgagtgccc ggcgatgatg gccccgctcg gcctgcgcta cgagagcgac gagcccggcg
      781 cccagaccct gttcaccgcg cgcccgcgcg cggggaagtg agacgatctg cgctcgctcg
      841 ccgccctgct cctgctgctc gcgcccgcct gtgacgacgg ggacgacgcc gcgatcctcg
      901 gtgaccccga atacgtccgc gaccccgcgc tcgacattcc gctgatcagc ggccacggcg
      961 aggcccgcag ccacaacatc ggccagaact gcatggcctg ccatcaggag cacggccccg
     1021 gcccgggccg cttcaccgcc gccggcacgc tgtacggcgc cgacggcacg cctcacgccg
     1081 acggcgcgat cgagctgcgc accggcgccg ccggcacggg cgatctggtg cgccgcatcg
     1141 aggccgacag caacggcaac ttctacacca cgcaggcgct gccgctgccc gacagcgcgc
     1201 tgttccccgc ggtctacgat cgcagcggcg cgctccgcaa ttacatgccc ttccccacct
     1261 cctccgcggc gtgcaacgtc tgccacgtcg gctccgcggt gatcctgatc ccggacccct
     1321 gagatcatgg tgtcgatcca tcgcatcgcc ctgctgctcg tcgccgtgtc ttgcacggga
     1381 tccgagcccg cggccgagcc gtgggtctgg gagctgccgg ccggcttccc cgagccgcac
     1441 gtgcccgacg acaacccgat gagcgtcgcc aaggtcgagc tcgggcgcca cctgttttac
     1501 gacaaacgcc tgtcgcacaa cgagacgatg gcctgcgcga gctgccacga gcaggcgcgg
     1561 gcgttcaccg acggcaaggc gacgaccgag gggtcgaccg gccacctcct gccgcgcaac
     1621 gcgatgagcc tgaccaacgt cggatatcta tcggtgtaca cctgggccaa cccggagctc
     1681 gcggacctcg aggagcaggc gctggtgccg atgttcgccg actatccgct cgagctcggg
     1741 acccagttca atacgccgca gatcctgcga cgtttcgccg acgacccgaa atatcaacag
     1801 ttgttcgccg acgccttccc cgaggatgac gaccccgtca ctacccagcg catcgtccag
     1861 gcgatcgcca gcttcgagcg cacgctgatc tccggcgacg ccgcctacga ccgcttcatc
     1921 tacggcggtg acgatgccgc gatgtcggcg tccgcccggc gcggcatgga cctgttcttc
     1981 agcgagcgcg ccgagtgcta tcattgccac gccggcccga acttctcgac ctcgtttcgc
     2041 agccgcacca gcgaggccgg gccgcccgac ttccagaaca acggcctgta caacctcgac
     2101 gccaccggcg cctacccgcc cggcaacggc ggcctgttcg agctcaccgg cgagccacgc
     2161 gaccagggca agttccgcgt cccgaccctg cgcaacgtca ccctcaccgc gccatacatg
     2221 cacgacggca gcatcgccac gctcgccgag gtcatcgagc actacgacgc cggcggccgc
     2281 gagatcacgg gcggcccgct cgccggcgat ggccgcatga accccaacaa gagcccgctg
     2341 gtgttcccga tggagctcga cgagcaggag aaggccgacc tcgtcgcgtt cctcgagagc
     2401 ctcaccgacc cgaccttcgc cagcgacccg cgcttcgccg ctccctgagc gcacgcggtc
     2461 gcggcgggcg ccgcgtccgt cacaaacaga cctcgcccgg ggcatgcacg cgtagtactc
     2521 tggcgtcatg ctcggcctcg cgctcgccaa cctcctgttt agcgccgaac cgccgc
//
LOCUS       DASWNJ010000853         1575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_192934, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000853 DASWNJ010000000
VERSION     DASWNJ010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192934"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..741
                     /locus_tag="VGB85_14485"
     CDS             16..741
                     /locus_tag="VGB85_14485"
                     /inference="COORDINATES: protein motif:HMM:NF017779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135288.1"
                     /translation="MNEAIDAGLRNVLAVAELAISELGLTRCPFPEALGALVGTHAGE
                     PLRMHTRRWRGPGFVALTMATITDAAGQLRSVTIIGLPAAGCPGPIVGVDLIGLGGAL
                     SLVAVDLAPTDDVTWQTHAAAPLAALHTAVETHVVIRRRPSFADEVFSPRALIAGVRR
                     GAELAVLDAVASFIEHAVGPRASPGKVAAERATAADDRVAAWCRAELRNRREHDALTR
                     LFGAVPATAYLDMLFGAGARTAA"
     gene            complement(696..1121)
                     /locus_tag="VGB85_14490"
     CDS             complement(696..1121)
                     /locus_tag="VGB85_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135289.1"
                     /translation="MTTTPRILLLERDSELAAVLDRVLTGHGYEVAVAHGGDEALRLA
                     RAEPPAVAVVDAGQLATLRALRSLPGWAELPAVVLVDGNHGALPRGDWALRRPFDFAA
                     LLDLIEGALVRAEPVRAVARARPIVQAAVRAPAPNSMSR"
     gene            complement(1244..>1575)
                     /locus_tag="VGB85_14495"
     CDS             complement(1244..>1575)
                     /locus_tag="VGB85_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135290.1"
                     /translation="MCALAATPWFTLVALPGGPGGATAAAARIAARWPGLVRVVDVVQ
                     GTCPEGHVGTDTVYDAAGEVDEVYAPRGAELCLVRPDGHIGARAPLAAAAAIEDHLAG
                     LLAGVVV"
BASE COUNT          202 a          662 c          526 g          185 t
ORIGIN      
        1 tgaggaagac acgccatgaa cgaggcgatc gacgccggtc tgcgcaacgt gctcgcggtc
       61 gccgagctgg cgatctcgga gctcgggctc acgcgctgcc ccttccccga ggccctcggc
      121 gcgctcgtcg gcacccacgc cggcgagccg ctgcgcatgc acacccggcg ctggcgcggc
      181 ccgggcttcg tcgccctcac catggcgacg atcaccgacg cggcgggtca gctgcgcagc
      241 gtcacgatca tcgggctgcc cgcggcgggc tgcccggggc cgatcgtcgg cgtcgatctg
      301 atcggcctcg gcggcgcgct cagcctcgtc gccgtcgacc tcgcgccgac cgacgacgtc
      361 acctggcaga cccacgccgc cgccccgctc gccgcgctgc acaccgcggt cgagacccac
      421 gtcgtgatcc ggcgccgccc gagcttcgcc gacgaggtgt tctcgccgcg ggccctgatc
      481 gccggagtcc gccgcggcgc cgagctggcc gtgctcgacg cggtcgccag cttcatcgag
      541 cacgcggtcg gcccccgggc gtcgcccggc aaggtcgctg ccgagcgcgc caccgcggcc
      601 gacgaccgcg tcgccgcctg gtgccgggcc gagctgcgca accgtcgcga gcacgacgcc
      661 ctgacccgcc tgttcggcgc ggtcccggcg accgcctacc tcgacatgct gttcggcgcc
      721 ggggcccgca ccgccgcctg aacgatcggc ctggcgcggg ccacggcccg gaccggctcg
      781 gcccgcacca gcgcaccctc gatcaggtcg agcagcgccg cgaagtcgaa cggccgccgc
      841 agcgcccagt caccgcgggg cagcgcgcca tggttgccgt cgacgagcac caccgccggc
      901 agctcggccc agccgggcag gctccgcagc gctcgcaacg tcgccagctg accggcgtcg
      961 accaccgcga ccgctggcgg ctcggcccgc gccaggcgca gcgcctcgtc cccaccgtgc
     1021 gccaccgcga cctcgtaccc gtgcccggtg agcacccggt cgagcaccgc cgccagctcg
     1081 gagtcacgtt cgaggagcag gatccggggc gtggtcgtca catcacaaaa cgtacaccaa
     1141 ccccccgtgg cggcccctga ctggcatttt aatcaaccaa ccggcgcccg atcctgtgcc
     1201 ccgcgaagac tccccgttcg attgcgaggc tggcgccggt ttttcacacc acgaccccgg
     1261 cgagcaggcc cgcgaggtgg tcttcgatcg cggccgccgc cgcgagcggg gcccgggcgc
     1321 cgatgtgccc gtcgggccgg accaggcaca gctccgcgcc tcgcggcgcg tacacctcgt
     1381 cgacctcgcc ggcggcgtcg tacaccgtgt ccgttccgac atgtccctcc ggacatgtcc
     1441 cctggaccac atcgacgacg cgcaccagcc ccggccagcg cgcggcgatc ctcgcggccg
     1501 ccgccgtcgc gcccccgggc ccgcccggca gcgccaccag cgtgaaccac ggcgtcgccg
     1561 cgagcgcgca catca
//
LOCUS       DASWNJ010000854         2530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_263697, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000854 DASWNJ010000000
VERSION     DASWNJ010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263697"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VGB85_14500"
     CDS             complement(<1..778)
                     /locus_tag="VGB85_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135291.1"
                     /translation="MANRSERETWTMRIGGLALTLVTGCFYSPTGSTPQDASTSSGGG
                     DEATATTDTTEVTPTTSQGETQYCADKDGDGFGEPDECITVPSGQTPPPGIVANPDDC
                     DDTSANTFPGAAPNDDTIACMKDEDGDDWGDVNPPVNIVPGTDCDDANMNAWAACGTC
                     ADADGDGWFSGCDAYPEGFPSEDCDDGDAKTFAGAAPNDDPQACMTDADDDDWGDNTP
                     TDPEAVPGTDCDDASNSTFPGAAMLDDATACMKDADDDGHG"
     gene            complement(929..>2530)
                     /locus_tag="VGB85_14505"
     CDS             complement(929..>2530)
                     /locus_tag="VGB85_14505"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0135292.1"
                     /translation="VADLDADGRLDIIVGSLRVDEQPPRSGEVHLGRDDGGFTDVTHA
                     SGLRPRATAFDADGDGDRDVYTVAGGLALQRGAPDTWLRVHPRQAASDRLASGAWITV
                     RDGDGRVVASGTAEAGVVALGLPDPDARYAVTVRFPGGASRTIADVPARSDLVVDDLD
                     GLAGGLARMRLWATGTWLRIVAVRDLLAPLLALALTLALARLRPRPWLPTFAGLALAG
                     LPLWLGFTVRGPATLAWLLAPLDLAAAALLTALIAAVTSARRRVRVGPFRLRERLGAG
                     AAGTVWRAERDGEDLALKLYEVDVMESADARERFFREARIGAEIQHPNLVQIVDAGRL
                     DDGRCYLVMKRIDGASLATRLTVGPRLPVASVVQIGIDIAGALAALHAADVVHRDVKP
                     ANIVLRRDGRAVLTDLGLARSALFRTVTRLDVAVGTLAYMSPEQAIGRPIDGRADLWS
                     LGVVLYELLTDRRPFSGQHEMELIYLLCNVDPPDLQAQDPTIPGDLAAIVMRCLAREP
                     QARHADAAALEAALAQVGRRLAAHT"
BASE COUNT          321 a          846 c         1000 g          363 t
ORIGIN      
        1 cgccatgacc gtcgtcgtcc gcgtccttca tgcacgccgt cgcgtcatcg agcatcgccg
       61 cgccggggaa ggtcgagttg ctcgcgtcgt cgcagtcggt gccgggcacc gcctcggggt
      121 cggtcggcgt gttgtcgccc caatcgtcgt cgtcggcgtc ggtcatgcac gcctgcgggt
      181 cgtcgttcgg ggccgcgccc gcgaaggtct tcgcgtcgcc gtcatcgcag tcttcgctcg
      241 gaaaaccctc cggataggcg tcgcagccgc tgaaccagcc gtcgccgtcc gcgtcggcac
      301 aggtgccgca ggccgcccag gcgttcatgt tggcatcatc gcagtcggtg ccgggcacga
      361 tgttgacggg cgggttcacg tcgccccagt cgtcgccgtc ctcgtccttc atgcaggcga
      421 tggtgtcgtc gttcggggcc gcgcccggga aggtgttggc gctggtgtcg tcgcagtcgt
      481 cggggttggc gacgatgccg ggcggcggcg tctggcccga aggtacggtg atgcactcgt
      541 cgggctcgcc gaatccatca ccatccttgt cggcgcaata ctgggtctcg ccctgcgagg
      601 tcgtcggcgt cacttcggtc gtgtcggtcg tggcggtcgc ctcgtcccca ccgccggagc
      661 tggtcgatgc gtcttgcgga gtcgagccgg tcggcgaata aaaacagccg gtcacgagcg
      721 tgagggccag gccgccgatc cgcatcgtcc aggtctcacg ctcgctcctg ttcgccatcc
      781 ccgcccgccc gttcatgaca cctcgcgcga gactaccaca gcgggtcggc cgcgctgtat
      841 cgagctcgtg tgatcgggac cggtggtgcg gtcgcggtcg ccggcgacgg tcattctttt
      901 tcgtaaaaaa gttcatgcat cctcgccatc acgtgtgcgc tgcgaggcgc ctgcccacct
      961 gcgcgagggc ggcttccagg gccgcggcgt cggcgtggcg cgcctgcggc tcgcgggcga
     1021 ggcagcgcat gacgatcgcg gcgaggtctc cggggatcgt cgggtcttgc gcctgcaggt
     1081 ccgggggatc gacgttgcac aggagataga tcagctccat ctcgtgctgg ccggagaagg
     1141 ggcggcggtc ggtcaacagc tcgtagagga cgacgccgag cgaccagaga tcggcgcggc
     1201 cgtcgatcgg gcggccgatc gcctgctccg ggctcatgta cgcgagggtg ccgacggcga
     1261 cgtcgaggcg ggtgaccgtg cgaaacaggg cgctgcgggc gaggccgagg tcggtgagca
     1321 cggcacggcc gtcgcggcgc aggacgatgt tcgcgggctt gacgtcgcgg tggacgacgt
     1381 ccgcggcgtg cagcgccgcg agggcaccgg cgatgtcgat gccgatctgc acgacggagg
     1441 cgacgggcag tcgcgggccg acggtcaggc gggtcgccag cgaggcgccg tcgatccgct
     1501 tcatgacgag gtagcagcgg ccgtcgtcga ggcgcccggc gtcgacgatc tggacgaggt
     1561 tggggtgctg gatctcggcg ccgatccggg cctcgcggaa gaagcgttcg cgggcgtcgg
     1621 cgctctccat cacatcgacc tcgtagagct tgagcgcgag gtcctcaccg tcccgctcgg
     1681 cccgccagac cgtccccgcc gcgccggcgc cgagccgctc gcgcaggcgg aaggggccga
     1741 cgcggacgcg ccggcgcgcc gaggtgacgg cggcgatcag ggcggtgagg agggcggccg
     1801 cggcgaggtc gagcggcgcg agcagccagg cgagggtcgc ggggccgcgg acggtgaagc
     1861 cgagccacag cggcagtccg gcgagcgcga ggccggcgaa ggtgggcagc cacgggcgcg
     1921 gacgcagccg cgcgagcgcg agggtgaggg cgagcgcgag cagcggggcg agcaggtcgc
     1981 gtactgccac gatccgcagc caggtcccgg tcgcccacag gcgcatgcgc gcgagcccac
     2041 cggcgaggcc gtcgaggtcg tcgacgacga ggtcgctgcg cgcgggcacg tcggcgatcg
     2101 tccgtgacgc gccgccgggg aagcggacgg tgacggcgta gcgggcgtcg gggtcgggca
     2161 ggccgagcgc gacgacgccg gcctcggcgg tcccggaggc gacgacccgg ccgtcgccgt
     2221 cgcggacggt gatccaggcg ccgctggcga ggcgatcgct ggcggcctgt cgcggatgca
     2281 cgcgcagcca ggtgtcgggg gcgccgcgct gcagcgcgag cccgccggcg acggtgtaga
     2341 cgtcacgatc gccgtcgccg tcggcgtcga aggcggtcgc gcgcgggcgc aggccgctgg
     2401 cgtgggtgac gtcggtgaag ccgccgtcgt cgcgcccgag gtggacctcg ccgctgcgcg
     2461 gcggctgctc gtccaccctc agagatccga cgatgatgtc gaggcgcccg tcggcgtcga
     2521 ggtcggcgac
//
LOCUS       DASWNJ010000855         7950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_651719, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000855 DASWNJ010000000
VERSION     DASWNJ010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7950
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651719"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="VGB85_14510"
     CDS             <1..725
                     /locus_tag="VGB85_14510"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0135293.1"
                     /translation="DHSVFRPFAAALAGFAQVVYLDQRGCGRSDRSEPARWNLDTWAD
                     DVRAVCDALEVVRPVVLGFSFGGIVAQAYATRYPDHPGKLILASTPARLRLDRMLPMF
                     ETLGGPRAADVARRFFTDPAATFPEYVEVCLPLYNQSPPDADALARAVLHLDVGSHFV
                     GGEMQTYDLRAALAGVRCPTLVLSGDLDPFATREDIRELIAALPPALVEARQIAQAGH
                     DVFADAPAEVAAALESFVTRTR"
     gene            complement(908..3109)
                     /locus_tag="VGB85_14515"
     CDS             complement(908..3109)
                     /locus_tag="VGB85_14515"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEY0135294.1"
                     /translation="MRAQRSGSRYPVQPPRTSTAPRRKIAVLGAGLGSLSAVFALTEA
                     ADWQDRFEVTIYQIGWRLGGKARSGRNLKAHNRSEAFGHQLWWGCYDNAFAMLRRCYK
                     ELARPAGAPLATFKEAWKPAESVFFGTGLEDHDRLWPLAVGRNDATPGEGDLPPSVWD
                     YVQVLLPWVLDCLPGIHPEYPNYATIAPLWQDQARSLLPALLRRAQSQGVDSFADFDP
                     QRPDYDRLLRNPAAPEGPQTSRQRRAWALPLVVGAEQSLRALEAAGAREHHPNFLGLL
                     DLALAVVRGIIHDGLDRHPQGFSAADDHDLRAWLRHHGASPASLATPVVTGFYDLLHA
                     PALAAGASLRMLLRILLAHKGALYYTPQADLGEVVFAPLYQLLRRRGVRFRFFHKVKE
                     LRVEDDRICAIRIGRQVELDPGVGDYEPLINVKGLLCWPSRPLYAQIVLGDSPMIQAL
                     DFESAESPEIEEFTLRRGADFDDVILGIPAPALPKICAQLVDKYPAWQQALAHADSVA
                     VQGAQLWLSPTWAQLAGHATPIHAGTLPGSQLPRWANRSPNVRLEQWPRELMPAAVCA
                     LDGPLPDPAPGQPGPGREQLRETVWTWLKSHPRRLWPEASLSATRELNWELLIDTNGR
                     TGDHRLAAQHCWLATAATDRATLTLPGHTRHRLRAADSGVKNLLLAGDWLADCLGVAE
                     SAVLTGFQASRALCGHPQVIPGEADGVDLEANAPGTFAGASQSMRMAAGAR"
     gene            complement(3312..3632)
                     /locus_tag="VGB85_14520"
     CDS             complement(3312..3632)
                     /locus_tag="VGB85_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135295.1"
                     /translation="MATINRFIQTEVCRDINGNIVSCDSREAVRPQPKIPVTDAPPPQ
                     PGEHLPDDVLRAQKAERFDEQIDVRAQLGEDLQAMRHMQGLQKDIGAMPQVNAGRFRS
                     KNRF"
     gene            complement(3654..6608)
                     /locus_tag="VGB85_14525"
     CDS             complement(3654..6608)
                     /locus_tag="VGB85_14525"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0135296.1"
                     /translation="MLLTPGSGARGRAPFEQELASTDAGLRAPQPEAADPHDTSTGIA
                     TPFERAGFDPGAELEQVNRENRFYGTSVDAPRVGKYVLLEHLGDGGMGSVYTVYNPDL
                     DRKSVIKLVRPDRSAPDSHLRLLREARLTATLSHPNVVVVHDCGEYRDQIYVVMEFIA
                     GVDLDKWRRRGPHPWREVLAVYHAAGLGLAAAHTRPIVHRDFTPRNAMLGDDGRVRVL
                     DFGLAHGRAKPASAPPAAPGPFDHLTASGAIVGTPRYMSPEQLCGAETDPRSDQFSFC
                     VCLWEALLDVLPFAGETMYEYNASVLTGAVRMPDVTRGVPGRVLRALRRGLAPRPDDR
                     WPDMPALLAALMPTPRPARWLLAPVAALGLAAGFMMMQAAPAQAAEQCAADLTGELHD
                     IWDDTCRDAVQTTLAARDPRAAAAVVARLDAFAARWAERRQDLCTAALAGGLPGAAQR
                     RGLQCLEGHRAVLGELATRLASGSAPDHGLTDLPDDDRLDACIDPLRIADGGAAASPA
                     GREDAARIDADLARASAALILGQYAESEAIAAAAVSRARALGHPPTLAAALYHHARAL
                     AYGPRALHAEPALREAAGLAERHDMHDLAADVYLRLIRVAHRNGERPADADIWFEVLG
                     HKLARLDEREGRRQAEALATLGYIALEAGDLASAERYAERSAAADEHANSFTRGLQEL
                     ERGRIARARGELTAARGHFERATALASEALGERHPKLAPMLLGLGEVALALADLDAAS
                     TAFDRARALLGADDPFLGEALFDLARVAEARGDLAGAHTLGGQARVALRGPVPRDIAR
                     HRVKLLGLVVDVAGAMHDRETAISAARELLAIPAADRPGAEQRFAQACYNAAGVALSF
                     QQPALALRLVAQAGRALADLPPTPQQEPLRVMLAIQETAALIGLGRPREAADVATRAL
                     QRWDPRDERDDAAIAELHLQLFDALQTFDHERASVHAVRALRYFADKTPDDPFVEILE
                     AWLRRYPAPDHP"
     gene            complement(6852..7406)
                     /locus_tag="VGB85_14530"
     CDS             complement(6852..7406)
                     /locus_tag="VGB85_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135297.1"
                     /translation="MPRSSPIATLALLAGACFYDLAPKTGATTDASSETTATTSAPTT
                     TSADPTTTSDITTTTGSTSGSSDTGGSTTSGADETTLETTGADLNLYGPCDMNAQCPS
                     GQQCLSIMETPGSFCSPACVGMGCPAAPPGVTAEPACVLTEARGREPVSCALVCDPSD
                     PTACPTSLVCKPLQMQRAGVCAAP"
     gene            complement(7450..>7950)
                     /locus_tag="VGB85_14535"
     CDS             complement(7450..>7950)
                     /locus_tag="VGB85_14535"
                     /inference="COORDINATES: protein motif:HMM:NF024896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0135298.1"
                     /translation="RVYLGEDVELGRRVRAAGYRVALGRPVLRTAPRHLSFSAAVDRF
                     ARWLAVVRLQRPALLASYPLVFAATTPALALAFTLPDVGVQGHVAGLVLLVARAVVFT
                     VGRGLAGLPRGPLAFVVDLVLADVVLWAALCRSLSARAVHWRGRVLAFDDRGRLIDDA
                     RAATLD"
BASE COUNT         1054 a         2615 c         3196 g         1085 t
ORIGIN      
        1 gtgatcattc ggtgtttcgc ccgttcgcgg cggcgctcgc cggcttcgcg caggtcgtct
       61 acctcgacca gcgcggctgc ggccgcagcg accgcagcga gccggcgcgc tggaacctcg
      121 acacatgggc cgacgacgtg cgggcggtgt gcgacgcgct ggaggtcgtt cggccggtgg
      181 tgctcgggtt ctcgttcggc gggatcgtgg cgcaggcata tgcgacgaga taccccgatc
      241 accccggcaa gctgatcctg gcgagcacgc ccgcgcggct gcgactcgat cgtatgctgc
      301 cgatgttcga aacgctcgga gggccacgcg cggccgacgt cgcccgccgc ttcttcaccg
      361 acccggccgc gaccttcccc gagtacgtcg aggtctgcct gccgctctat aaccagagcc
      421 cgccggacgc ggacgcgctc gcgcgtgctg tcctccacct cgacgtcggc agccacttcg
      481 tcggcgggga gatgcagacc tacgacctgc gcgccgcgct cgcgggcgtg cgctgcccga
      541 ccctggtgct gtccggcgac ctcgacccgt tcgccacccg cgaggatatc cgcgagctga
      601 tcgccgcgct gccgccggcg ctcgtcgagg cccggcagat cgcgcaggcc gggcacgacg
      661 tcttcgcgga cgcgcctgcg gaggtcgcgg cggcgctcga gtcgttcgtc accaggacgc
      721 ggtagcgcgg cgcctcccgg acgcgctgac aggaggccag acgagtctcg cgcggggcct
      781 gcatgcacgc cgctcccaaa ccgacgggga ggtcggcgat cgagtcgttc gtcaccacga
      841 cgcggtggcg cggctcctcg ccggacgcga tgccagcgtc gggacgagga gcagctggcg
      901 cacgcctcta gcgggcaccg gcggccatgc gcatcgactg cgaggcgccg gcgaaggtgc
      961 cgggggcgtt ggcctcgagg tcgacgccgt cggcctcgcc ggggatgacc tgggggtggc
     1021 cgcacagggc gcgggaggcc tggaagccgg tcagcacggc ggactcggcg acgccgaggc
     1081 agtcggcgag ccagtcgccg gcgagcagga gattctttac gccgctgtcg gcggcgcgca
     1141 ggcggtggcg ggtgtggccg ggcaaggtga gggtggcgcg gtcggtcgcg gcggtcgcca
     1201 gccagcagtg ctgggcggcg aggcggtggt cgccggtgcg gccgttggtg tcgatcagca
     1261 attcccagtt gagctctctc gtggccgaga gcgaggcctc gggccacagg cggcgaggat
     1321 ggctcttgag ccaggtccac acggtctcgc gcagttgctc gcggccgggg ccgggctggc
     1381 cgggggcggg gtcgggcagc gggccgtcga gggcgcacac ggcggcgggc atgagctcgc
     1441 ggggccattg ctcgaggcgc acgttgggcg agcggttggc ccagcgcggg agctgggagc
     1501 cggggagggt gccggcgtgg atcggtgtcg cgtggccggc gagctgggcc cacgtcggcg
     1561 agagccacag ctgggcgccc tgcacggcga cgctgtcggc gtgggcgagg gcctgctgcc
     1621 acgcgggata cttgtcgacc agctgcgcgc agatcttcgg cagcgcgggg gcggggatgc
     1681 cgaggatgac gtcgtcgaag tcggcgccgc gacgcagcgt gaactcttcg atctccggag
     1741 attcggcgga ctcgaagtcg agggcctgga tcatcgggct gtcgccgagc acgatctggg
     1801 cgtagagcgg ccggctgggc cagcacagca ggcccttgac gttgatcagc ggctcgtagt
     1861 cgccgacgcc ggggtcgagc tcgacctgcc gaccgatccg gatcgcgcag atgcggtcgt
     1921 cctcgacgcg cagctccttg accttgtgaa agaagcggaa gcggacgccg cggcggcgca
     1981 ggagctgata cagcggggcg aacacgacct cgccgaggtc ggcttgcggg gtgtaataaa
     2041 gcgcgccctt gtgcgcgagc aggatcctca ggagcatgcg caggctggcg ccggcggcca
     2101 gcgcaggagc gtgcagcagg tcgtagaagc cggtcacgac cggggtcgcc agcgaggccg
     2161 ggctggcgcc gtggtggcgt aaccatgcgc gcaggtcgtg gtcgtccgcc gcggagaagc
     2221 cctgcgggtg gcggtcgagg ccgtcgtgaa tgatgccgcg cacgacggcg agcgcgaggt
     2281 cgaggaggcc gaggaagttg gggtggtgct cgcgggcgcc ggcggcctcg agcgcgcgca
     2341 ggctctgctc ggcgccgacg acgagcggca gggcccaggc gcggcgctgg cgagacgtct
     2401 gcgggccctc gggcgcggcc gggttgcgca ggaggcggtc gtagtcgggg cgctgggggt
     2461 cgaagtcggc gaagctgtcg acgccctggc tctgggcccg ccgcagcagc gcgggcagca
     2521 ggctccgggc ctggtcctgc cacagcggcg cgatggtcgc gtagttgggg tattcggggt
     2581 ggatgccggg caggcagtcg agcacccacg gcagcaggac ctgcacgtag tcccacacgg
     2641 acggcggcag gtcgccctcg ccaggggtgg catcattgcg gccgacggcg agcggccaca
     2701 ggcggtcgtg gtcctcgagg ccggtgccga aaaaaaccga ctcggcgggc ttccaggcct
     2761 ccttgaaggt cgccagcggg gcccccgccg gccgggccag ctccttgtag cagcggcgca
     2821 gcatggcgaa ggcgttgtcg tagcagcccc accacagctg gtgaccgaag gcctcgctgc
     2881 gattgtgggc cttgaggttg cggccgctgc gggccttgcc gccgagccgc cagccgatct
     2941 gataaatggt gacctcgaag cggtcctgcc agtcggccgc ctcggtcagg gcgaacacgg
     3001 ccgacagcga gccgagcccg gcgccgagca cggcgatctt tcgccgcggc gcggtcgagg
     3061 tgcgcggcgg ctggaccggg tagcgcgagc cggatcgttg cgcgcgcatc gacagggagg
     3121 acgatgatgc ggacgacgcg gatgaagatg atgaagagga cgagcgcggg ggggatcctg
     3181 gtcggtcggt catgagctca cgtcccgggg ggcaacgccg ggagtgtagc tcggtttcga
     3241 gcggaggcgc aaaagcgact tcgccggcta ccccggcgaa gtcgtgacgc gcagtccagt
     3301 gcggggggcg gctagaaccg gttcttgctc ctgaaccttc cggcgttgac ctggggcatc
     3361 gcgccgatgt ccttctggag gccctgcatg tgcctcatcg cctgcaggtc ctcgccgagc
     3421 tgcgcgcgca cgtcgatctg ctcgtcgaag cgctcggcct tctgggcgcg gagcacgtcg
     3481 tcggggagat gctcgccggg ctgagggggc ggggcgtccg tgaccgggat cttgggctgc
     3541 gggcgcacgg cctcgcggct gtcgcacgag acgatgttac cgttgatgtc tcggcacact
     3601 tcggtctgaa tgaatctgtt tatggttgcc atggggtgtc ctgtctttgc tcgttacggg
     3661 tgatcgggtg ccgggtagcg tcgcaaccag gcctcgagga tctcgacgaa cgggtcgtcc
     3721 ggcgttttat ctgcgaagta tcggagggct cgcacggcgt gcacgctcgc gcgctcgtga
     3781 tcgaaggtct gcagggcgtc gaacaattgt aggtgcaact cggcgatcgc ggcgtcgtcg
     3841 cgctcgtctc gcgggtccca gcgctgcagc gcgcgcgtgg cgacgtctgc ggcctcgcgc
     3901 gggcgaccga gcccgatcag ggcggcggtc tcctggatcg cgagcatcac ccgcagcggc
     3961 tcctgctgag gcgtgggcgg gaggtcggcg agcgctcgcc cggcctgcgc gacgaggcgc
     4021 agggccaggg caggctgctg aaaggacagg gcgacgcccg cggcgttgta gcaggcctgc
     4081 gcgaaccgct gctcggcgcc tggccggtcc gccgcgggga tcgcgagcag ctcgcgggcc
     4141 gccgagatcg cggtctcgcg gtcgtgcatg gcgccggcga cgtcgacgac gaggccgagc
     4201 agcttgacgc gatggcgagc gatgtcgcgg ggcacggggc cgcgcagggc gacccgcgcc
     4261 tggccgccga gcgtgtgggc gccggccagg tctccgcggg cctcggcgac gcgggccagg
     4321 tcgaacagcg cctccccgag gaacgggtcg tcggcgccga gcagcgcgcg ggcgcggtcg
     4381 aaggcggtgc tggcggcgtc gaggtcagcg agcgcgagcg cgacctcgcc gagtccgagg
     4441 agcatcggcg cgagcttggg gtggcgctcg ccgagggcct cgctcgccag ggcggtcgct
     4501 cgctcgaagt gcccccgcgc cgccgtcagc tcgccgcgcg cgcgggcgat ccggccccgc
     4561 tccagctcct ggaggccgcg cgtgaacgaa ttggcgtgct cgtcggcggc ggcgctgcgc
     4621 tcggcgtagc gctcggcgga ggcgaggtcc cccgcttcga gggcgatgta gccgagcgtg
     4681 gcgagggcct cggcctgacg ccgaccctcg cgctcgtcga ggcgggcgag cttgtggccg
     4741 aggacctcga accatatgtc ggcatccgcg ggccgctcgc cgtttcggtg ggcgacgcgg
     4801 atcagtcgca gatacacgtc ggcggcgagg tcgtgcatgt cgtggcgctc ggcgaggccg
     4861 gcggcttcac gcaaggcggg ctcggcgtgc agcgcccgcg ggccgtaggc cagggcgcgg
     4921 gcgtggtgat agagggcggc ggcgagggtc ggcgggtggc cgagggcgcg ggcccgggac
     4981 accgcggcgg cggcgatcgc ctcgctctcg gcgtattgcc cgaggatcaa cgcggcgctg
     5041 gcacgggcga ggtcggcgtc gatgcgggcg gcgtcctcgc gtccggccgg cgaggccgcg
     5101 gcgccgccgt cggcgatccg cagggggtcg atgcaggcgt cgaggcggtc gtcgtcgggg
     5161 aggtcggtga gcccgtggtc gggcgcgctg cccgaggcca gccgagtcgc cagctcgccg
     5221 agcacggcgc ggtgaccttc gaggcactgc aagccgcggc gctgggcggc gcccggcaag
     5281 ccgcccgcga gggccgcggt gcacaggtcc tggcggcgct cggcccagcg ggcggcgaag
     5341 gcgtcgaggc gggcgacgac ggcggcggcg gcccgggggt cgcgcgcggc gagcgtggtc
     5401 tggacggcgt cgcggcaggt atcgtcccag atatcatgca actcgccggt gaggtcggcc
     5461 gcgcattgtt cggcggcctg ggcgggggcg gcctgcatca tcatgaagcc ggcggcgagg
     5521 ccgagcgcgg cgaccggcgc gagcagccag cgcgcgggcc tcggtgtggg catcagcgcg
     5581 gcgagcaggg cgggcatgtc gggccagcgg tcgtcgggcc tgggggcgag gccgcgacgc
     5641 agggctcgca gcacgcggcc gggcacgccg cgggtgacgt cgggcatgcg cacggcgccg
     5701 gtcaggacgg aggcgttata ctcgtacatg gtctcgcccg cgaatggcag cacgtcgagc
     5761 agcgcctccc acaggcacac gcagaagctg aactgatcgc tgcgcgggtc ggtttcggcg
     5821 ccgcacagct gctcgggcga catgtagcgc ggggtgccga cgatcgcccc gctggcggtc
     5881 aggtggtcga aggggcccgg ggcggccggt ggcgcgctgg ccggtttggc gcgcccgtgg
     5941 gcgaggccga agtcgaggac gcggacgcgg ccgtcgtcgc cgagcatcgc gttgcgcggg
     6001 gtgaagtcgc gatgcacgat cggccgcgtg tgcgcggcgg cgaggccgag gccggcggcg
     6061 tgatacaccg cgagcacctc acgccacggg tgcggcccgc ggcgccgcca cttgtcgagg
     6121 tcgacgccgg cgatgaattc catcaccacg tagatctggt cgcgatactc gccgcagtcg
     6181 tggacgacga cgacgttggg gtgcgagagc gtggcggtca gccgggcctc ccggagcagc
     6241 cgcaggtggc tgtcgggcgc cgagcggtcg ggtcgcacga gcttgatgac gctcttgcgg
     6301 tcgaggtccg ggttgtagac ggtataaacc gaacccatgc cgccatcgcc gaggtgctcg
     6361 agcaggacat acttgccgac cctgggcgcg tccacggagg tgccgtaaaa gcgattctcg
     6421 cggttcacct gctcgagctc ggcgccaggg tcgaagccgg cgcgctcgaa cggagtcgcg
     6481 atgccggtgc tggtgtcgtg tgggtcagcc gcctccggtt ggggggcgcg aagccccgca
     6541 tcggtcgagg cgagctcctg ctcgaacggg gcgcgtccac gggcgcctga gccaggagtg
     6601 aggagcatcg cgcgatcacc gtatcgacgc ggtctgaggt cgacaacacc cgcttccctg
     6661 ctaattctgc gcgcggtgtc gaccatcgat ccgcgcggag gtcgtccgcg tttgtgcggt
     6721 cgatcgggcg aaccaatgtt ccgggtgtgc gcaggggcag ggtcgggtac ttacaatatc
     6781 cgcgcagacc cggcaccccg gcgctcgtgg atacccgggg gacccgtact gccaggaccc
     6841 gggatgacgc gtcacggcgc tgcgcacacg ccggcccgct gcatctgcaa gggcttgcat
     6901 acgagacttg tgggacacgc ggtcgggtcc gacgggtcgc agaccagcgc gcagctgacc
     6961 ggctccctgc cccgcgcctc ggtcagcacg caggccggct cggccgtgac gccgggcggc
     7021 gccgccgggc agcccatgcc gacgcaggcc ggcgagcaga agctgccagg ggtctccatg
     7081 atcgacagac attgctggcc cgacggacac tgcgcgttca tgtcgcacgg accgtagagg
     7141 ttgaggtcgg ccccggtcgt ctcgagcgtc gtctcgtcgg cgccgctcgt cgtcgagccg
     7201 ccagtatcgc tgctgccgct cgtcgagccg gtcgtcgtgg tgatatcgct ggtggtcgtg
     7261 ggatctgcgc tcgtggtcgt cggggcgctc gtggtcgcgg tggtctcgct gctcgcgtcc
     7321 gtcgtcgcgc cggtcttggg cgcgaggtcg tagaagcagg cgcccgcgag cagggcgagt
     7381 gtagcgatcg gcgagctgcg aggcatgggc atcgatagca cgacccgggc cggccgccgc
     7441 gcggccctgt cagtccagcg tcgccgcgcg tgcgtcgtcg atcagccgcc cccgatcgtc
     7501 gaacgcgagc acgcggccgc gccagtggac cgcgcgcgcg gacaggctgc ggcacagcgc
     7561 agcccacagc acgacgtcgg cgagcacgag gtcgacgacg aacgcgagcg ggccgcgggg
     7621 cagcccggcc agtccgcgac cgaccgtgaa cacgacagcg cgcgcgacga gcagcacgag
     7681 gccggcgacg tggccttgca cgccgacgtc cggcagcgtg aacgccagcg ccagcgccgg
     7741 ggtggtcgcg gcgaacacga gcgggtacga ggccagcagc gcgggccgct gcaggcggac
     7801 cacggcgagc cagcgggcga agcggtcgac ggccgcggaa aacgacaggt gtcgtggtgc
     7861 ggtccgcagc acgggtcgac cgagcgcgac gcggtatccg gccgcgcgca cgcgccggcc
     7921 gagctcgacg tcctcgccga ggtagacgcg
//
LOCUS       DASWNJ010000856        13189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_508648, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000856 DASWNJ010000000
VERSION     DASWNJ010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 13189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13189
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508648"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /locus_tag="VGB85_14540"
     CDS             <1..1276
                     /locus_tag="VGB85_14540"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0135299.1"
                     /translation="GRLHAMRGRSTEAATAFAAALAEAPDAAAAIALAELRVKIGAAD
                     PWTPIVKTEERLRGDPRERVRPQLRALRLAHANLLAETGRKDQAVAVLDAALLADPDD
                     NAAQLRRGVLAIEQGRSEAGRADLLAVYDRTGGYPGLIGPLGRLYLREGDLARLADLV
                     QTHAEDPQASDEVVIMYALLRLAQGDRDAADQNIDKVLQRSPGAWEAHLAKARVFYER
                     ERLTEALAEIRLARPRVPDAEVELWTGKIAERSGKPQDALAAFRRARQLDPGLLEAGY
                     LLARTLLAQGLAREAVTELQAITRAPHAPVGAHLTLGLAFRERDQLAEALQSLTRAIE
                     QDPNPEEALYWAGRTAVELGRPAEAVSHLGRAVLAAAGTSWLADAHLWLGRAQHRLGH
                     RTEARAALTRYLQLAGPKATARAEAERVLRER"
     gene            1323..1409
                     /locus_tag="VGB85_14545"
     tRNA            1323..1409
                     /locus_tag="VGB85_14545"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1357..1359,aa:Ser,seq:tga)
     gene            complement(1443..2468)
                     /locus_tag="VGB85_14550"
     CDS             complement(1443..2468)
                     /locus_tag="VGB85_14550"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEY0135300.1"
                     /translation="MPRPVANPPNPWLSTQVEYLEDVPMAALEVFEEDARSIVNRNDS
                     PDVPFNWSVNPYRGCYHGCAYCYARPTHEYLGWGAGTDFDRKIVVKRNAPELLRRAFE
                     AKGWKGEVLAFSGVTDCYQPLEASFQLTRRCLEVCLEYRNPVGIVTKGALVARDTALL
                     AELGAEAGCIVSISIPFADDEGGRQIEPFASTISKRLAALEQLSAAGVRTGVGVSPVI
                     PGLNDSQIPTILKRARDAGAQHAFMTMLRLPGSVLPVFQERLQAAVPLRAQAVLSAIR
                     DIRGGGMNRSSFGERMTGEGPRWQAIEQLFTTTARRLGFATDDSPIHPARNAFRIPSR
                     QRSLFGD"
     gene            complement(2586..4046)
                     /locus_tag="VGB85_14555"
     CDS             complement(2586..4046)
                     /locus_tag="VGB85_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135301.1"
                     /translation="MLANFLLSVVSTAGPASSGSAAEERLEKDKAIIRVLDRATDDYK
                     LFVSGVCRTGDHDTADLAARAAAMSEERAIRTRDLTELVDPADRAEYERAAAELFIEA
                     ADFWLRALTCRPSRSLYLEHAELPLLDAERLTQGDAGLSAAITERRKRIALYRRREVE
                     RPEVSPTCRQGDLKSTELVALHAAQTRERAIQARTPEEAATLAGAPDPARPATGESGV
                     QHESKAAHLALEAAGLWVRAWECHRVSGYLDNATLLLAEAEHLTPGDALVRRIRGLRR
                     TIALHRAKLWTDRFSLRIELGYGSGRLAWLKHQTYGSIYEYGYRAHGGAYFGLSAMLR
                     APPRTGPVNLLFGPYYTYWRAAQHSFLPETKSANVHEFGAKTEVSVGFTPKVAKFFTL
                     HPSLEVGLQYINFDDRPIPGQLVDPEYIDMAGGTVGMSLGVCFIYSSVCAMSRVHSVP
                     QAWKQGIPTLRGGIAIDLVRLTQAVLERKSLRLQAK"
     gene            complement(4052..6670)
                     /locus_tag="VGB85_14560"
     CDS             complement(4052..6670)
                     /locus_tag="VGB85_14560"
                     /inference="COORDINATES: protein motif:HMM:NF044599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0135302.1"
                     /translation="MSVIDPPSNPFAVGNAVGGTPYFVGRSDIIHKVVSVLRRPLEPG
                     LVLFGQRRIGKSSILRELEEQLPKLGPWKPVFFDLQRHTRATIEEILEDLASIIATAL
                     GLPDPVFVGEDIMGAFCKTWLPEVLKARPDGSRLVLLLDEFDVLTDPKTNSASKRLFE
                     FLRSIFDDNTLRTRVAAIYAMGRTMEDLDISTGPLFRGLRTEPVSTLGRQDFDLLLAR
                     GTHTGGIEWGHGVIDAIWRLTAGHPMLTQLLASRAWESAMLSKEPRVDIRQLEELTHS
                     VLRESHNILSWLWEGLTPACRVVASAFAEHGSRSLTAEELEKLLLQSGVRIMMGQLAE
                     APARLRDWDVLDETQTSPRRYRFKVELLRMWVEKYRPFASVREYIDQVNPEADADYRR
                     ALQEWNTGERSPADIDLIIQRLGLVLNEGKGNPNHVGATELLAEVYRSQGSLDRAIQV
                     IARLLPLQTAALRPRYVQLLLTKAENLAGDEEQEASCLQCFQMILEVAPGTSEAITGM
                     RNIWREQGRRAHAAGRLAEARDLYARAEQPTLVSEVDLEIVRIEGEQALSRVRELASG
                     EKFDEAVELLDSQQHALRSVMGPELDSERQEIERARSLLAYYLSGCAAASEGRRPQAL
                     ASFGHVLAVNPDYKDVQSRVGGLFTPPRTLAHRLALPLGGAFAVALVLLALKDVATAL
                     VSATRDCPAEVARAAFPSEVPAGAASTTPYCPATTGEPGVSPTAPATTPVVTIAKPEH
                     IVEKAKPGGVVDAHDVRRSKKRPAPAHKPPPAEAPPPAKLTMDQVAVGFQGVIQMFCR
                     AKVTSSTSMLTLEFSIDVDTGAVRRVRKVESDDKLSDPNCIEANEVAITRAISFAAAQ
                     SRVTGYKHHYWVKPKG"
     gene            complement(6661..7992)
                     /locus_tag="VGB85_14565"
     CDS             complement(6661..7992)
                     /locus_tag="VGB85_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135303.1"
                     /translation="MPSGRTTLLRALQQRLQARDCRVAFIDVQTLDSSTTADRFWSSV
                     LGQFGGARGGNLSFRGIERLLAEPGKRHILLIRDLQALVHLPDFRRGDPWGKLRSLTQ
                     TPRFTLVATSRIDLVALTTATQEWMYGSPFFNGMRELVLGPFTRSDALAYLGHHRPYL
                     TPEDHEWILRCTGGHPKMLRFLGDALEQSLAAGNRGSGARLQAIAGCRQQLRVVLQNA
                     WLFLPWQERWILLRTALAQRSAVAFSRPGAPLREIQDEPLAPGALIRLLESFDRDSLR
                     RLLLHVLPARDLAALPGDSVSDLAYSTAAALLLDRLGAVPSFLRKRLELEHPGHGGRG
                     DAHVWGAPFTSGAPWQPTVETCDRLIPRGILAETRAAPGLEITPPLLYWWILDQLHAI
                     AAGTDLRQWLAQHGLAGHVSSGEAEALVATAQRMEGLLGRGAAPLMEAQCQ"
     gene            complement(8431..8589)
                     /locus_tag="VGB85_14570"
     CDS             complement(8431..8589)
                     /locus_tag="VGB85_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135304.1"
                     /translation="MLRRSVVNAYFDVQNIYNRQNPEDLIYNPHFTVVKTTVGVPILP
                     VFGVRVEY"
     gene            complement(8993..9784)
                     /locus_tag="VGB85_14575"
     CDS             complement(8993..9784)
                     /locus_tag="VGB85_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135305.1"
                     /translation="MYTRLGFTTLALLLFTACGDNGSNTSDTDADSTGAADSTGTTPD
                     TTSDTDATTDTAGSDSATGDTTGDVPTTGVTSKPNDSAGDDTSDTDATTDATTDATTE
                     GETTDPVDPEIVSGCEAYCARWTECGFPQDEAECTAGCVEDLGFYEGACKDANKDMLA
                     CATALTCEQLLEGPAEACAEQEEAVAQACQGSEEEGCSVLTGAFGDECDLTIECEEEP
                     TKKMLCTGETCTCTVGDEKVGECQADEACMDFDALEAKGKTCCGF"
     gene            complement(9953..10666)
                     /locus_tag="VGB85_14580"
     CDS             complement(9953..10666)
                     /locus_tag="VGB85_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135306.1"
                     /translation="MSTALAHPALSATTAAPAPLPLPGPRLRSLLVCAAERARHPHAE
                     ARELAPGLHELVALRRIDGTLELPARGVLRFHGLDPDAAFTRARLGTCGEPLEFGRAG
                     PLPGVHAYILGGPGGRDTAVAALELAAHIDPGAAGIVLAVPARGTALFLAPHARGLCR
                     VLARIGRFRQFWSSDGDDAPVHVRDSLRFLATWTANAYARLADPVSPALYWHRPGHPL
                     AVLIADAREPLAIPNELAA"
     gene            10784..11614
                     /locus_tag="VGB85_14585"
     CDS             10784..11614
                     /locus_tag="VGB85_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0135307.1"
                     /translation="MSRDLLLLLGLVALTGCAGEVTAFAAMVFSMSLGAWLVGRGHQR
                     RALLEAVQLRRAIATGRHREIEQHLRRELALAAAGEAVSVERQWLARAQLGGLLVAEW
                     RLDEAREIYTTTTAKLSPHLQALAAFGRHELDVLTREPDTVILDCIRSDRDACLRHIP
                     TPYQSTVARTWAALEGLCLARMGRPREAVPLLARGLECLGYNPARVVYLFHLAQAHEQ
                     LGDRQLAASRYEQAMHAFPGTRLASEARARCHALDAGRGDHLFRGMLPEPPMAARERG
                     "
     gene            11739..12494
                     /locus_tag="VGB85_14590"
     CDS             11739..12494
                     /locus_tag="VGB85_14590"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0135308.1"
                     /translation="MSEGFVRKRLHSAAETMSHSLGYARHLLRGNRVAGDLSWVQPGT
                     RPVVLLHGFLGTRGTMQPLTRRFQADGRVVFSYHHGTFQLGSLRSSSEELIHRLRDLV
                     ENLELDRVDVLGFSMGGLVALHAVKFLQGQKWIRRLVLLGTPLAGSWMSLAGIATVGL
                     VSPSVWQVRPGCQFLDELRAAPLPHGVRVRQIHADADAFCRNPGPVEGVDPEHDYLVL
                     PGGHSSLVIAQHFYAAAREFLDETDEAEIEVRR"
     gene            complement(12505..>13189)
                     /locus_tag="VGB85_14595"
     CDS             complement(12505..>13189)
                     /locus_tag="VGB85_14595"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0135309.1"
                     /translation="TSGGWNLGELQAMGLVGALADEDEQRRQARVDRLLATAEMSIRD
                     LLTVDEGLRGAGLRPPERARVAAAEPAPEPDVAIVPPVLEDTPDAAPDRPAKPGKPVL
                     ARNQRDPARARKLADEGAKALRAGNRSEAENLFHQAISFDNRNAKALMGLSDVYFDTG
                     AKVKAVQFAELAVEAAPQSRASNLKLGDAYYNVLRYRDALKHYEKAQELGEESAQGRI
                     DKVKARIGD"
BASE COUNT         1960 a         4387 c         4756 g         2086 t
ORIGIN      
        1 cggtcgcctg cacgcgatgc gcgggcgcag cacggaggcg gcgacggcct tcgcggcggc
       61 gctggccgag gcccccgacg cggccgcggc gatcgccctc gccgagctgc gcgtcaagat
      121 cggcgccgca gacccctgga cgccgatcgt caagaccgag gagcgcctgc gcggcgatcc
      181 gcgcgagcgc gtccgtccgc agctgcgcgc gctccggctg gcgcacgcca acctgctcgc
      241 cgagacaggt cgcaaggacc aggcggtcgc cgtgctcgac gcggcgctgc tcgccgaccc
      301 cgacgacaac gcggcccagc tgcgccgcgg ggtgctggcg atcgagcagg gccgcagcga
      361 ggccgggcgc gccgacctgc tcgccgtgta cgaccgcacc ggcggctacc ccgggctgat
      421 cgggccgctc gggcggctgt acctgcgcga gggtgacctg gcccggctcg cggatctcgt
      481 gcagacgcac gccgaggacc cgcaggcgag cgacgaggtc gtgatcatgt acgcgctgct
      541 gcgcctggcc cagggcgacc gcgacgccgc cgaccagaac atcgacaagg tgctgcagcg
      601 cagcccgggc gcgtgggagg cccacctcgc caaggcccgc gtgttctacg agcgcgagcg
      661 attgaccgag gcgctcgccg agatccggct cgcgaggccg agagtgcccg acgcggaggt
      721 cgagctgtgg accggcaaga tcgccgagcg cagcggcaaa ccgcaggacg ccctggcggc
      781 gtttcgccgg gcccgccagc tcgaccccgg cctgctcgag gcgggctacc tgctcgcccg
      841 tacgctgctg gcccagggcc tcgcgcgcga ggcggtgacc gagctgcagg cgatcacccg
      901 ggccccgcac gcgccggtcg gggcgcacct gaccctcggc ctcgcgttcc gcgagcgcga
      961 ccagctcgcc gaggccctgc agagcctgac ccgggcgatc gagcaggacc cgaacccgga
     1021 ggaggcgctg tactgggccg gacgcaccgc ggtcgagctc ggacgaccgg ccgaggccgt
     1081 gtcccacctc ggccgggcag ttttggcggc cgccggcacc agctggctcg ccgacgccca
     1141 cctgtggctc ggccgggccc agcaccgcct cggccaccgc accgaggcgc gggccgctct
     1201 gacccgctac ctccagctgg caggccccaa ggcgaccgcc cgggccgagg ccgagcgagt
     1261 gctacgcgag cgctgaaagc ccctggacga cccgcccgcg cttccccata ctccgggccg
     1321 ccggaggagt ggccgagcgg tctaaggcag cggacttgaa atccgtcgtg ggggtgactc
     1381 caccgagggt tcgaatccct cctcctccgc tcttttcttt cttctttaaa attaaataaa
     1441 tatcagtcgc cgaacagcga gcgctggcga ctcgggatcc gaaacgcgtt gcgggcgggg
     1501 tggatcggtg agtcgtcggt ggcgaagccg aggcgcctgg cggtcgtcgt gaacagctgc
     1561 tcgatcgcct gccagcgcgg gccctcgccg gtcatgcgct cgccgaagct gctgcggttc
     1621 atgccgccgc cacggatgtc gcggatcgcc gagagcacgg cctgggcgcg caggggcacc
     1681 gcggcctgca agcgctcctg aaacaccggc agcaccgagc ccggcaggcg cagcatcgtc
     1741 atgaaggcgt gctgggcccc ggcatcgcgc gcgcgcttca agatcgtcgg gatctggctg
     1801 tcgttgagcc cggggatcac cggggagacg ccgacgccgg tgcgcacgcc cgcggcgctg
     1861 agctgctcga gcgctgcgag gcgcttgctg atcgtcgagg cgaagggctc gatctgccgc
     1921 ccaccctcgt cgtcggcgaa ggggatgctg atgctgacga tgcagccggc ctcggcgccg
     1981 agctcggcca gcaaggcggt gtcgcgggcc accagcgcgc ccttggtgac gatgccgacc
     2041 gggttgcgat actcgaggca gacctcgagg cagcgccgcg tcagctggaa ggaggcctcg
     2101 agcggctggt agcagtcggt gacgccggag aacgcgagca cctcaccctt ccaccccttg
     2161 gcctcgaagg cgcggcgcag taactccggc gcgttgcgct tgacgacgat cttgcggtcg
     2221 aagtcggtgc cggcgcccca gccgagatac tcgtgggtcg gccgcgcata acagtaagcg
     2281 cagccgtgat aacagccgcg gtacgggttg acgctccagt tgaacggcac gtccgggctg
     2341 tcgttgcggt tgacgatcga tcgcgcgtcc tcctcgaaga cctcgagcgc ggccatcggc
     2401 acgtcctcga ggtactcgac ctgcgtggac agccaggggt tcggcgggtt ggcgaccggg
     2461 cgcggcatgg cagcggtgca gcgtagcgca cggatgcacg cagcgacatg tcccccggga
     2521 catgtcacgc cagcgggcgc ggcgccgtct ggggccaccc tcgacacgcg ccgcacatgc
     2581 agcgtttact ttgcctgcag acgcagcgac ttccgttcga gcaccgcctg tgtcaggcgg
     2641 acgaggtcga tcgctatccc ccctcgtagc gtcggtattc cctgcttcca ggcttgcggc
     2701 accgagtgaa cccgcgacat cgcacagacg gagctataga tgaagcaaac cccgagcgac
     2761 atgccgacgg ttccgcccgc catatcgata tactccgggt cgacgagttg cccagggatg
     2821 ggccgatcgt caaaatttat atactggagg ccgacctcga ggcttggatg gagcgtgaag
     2881 aacttcgcca ctttgggggt gaagccgacc gaaacctccg tctttgcgcc gaactcgtgg
     2941 acgttcgcgc tcttcgtctc ggggagaaag ctgtgctgcg cggcgcgcca atatgtgtag
     3001 tagggcccga agaggaggtt gacgggaccg gtcctgggtg gtgcgcgcag catcgccgac
     3061 aggccgaagt atgcgccgcc atgagcccgg taaccgtatt catagatcga cccgtacgtt
     3121 tgatgcttca accaagcgag acggccggac ccgtatccaa gttcgatccg cagcgagaag
     3181 cgatccgtcc acagctttgc gcggtgaagc gcgatcgttc ttcgcaggcc ccgaatcctg
     3241 cggacgagcg catctccagg cgtgagatgc tcggcttcgg ccagaagcag cgtggcattg
     3301 tcgaggtacc ccgacacgcg atgacattcc caggcccgta cccagagacc ggcagcctcc
     3361 aacgccaggt gggccgcctt ggactcgtgc tggacacctg actcaccggt agccggtctc
     3421 gccgggtctg gcgcacccgc gagggttgcc gcttcttctg gcgtccttgc ctgaatcgcc
     3481 cgctccctgg tttgcgcagc atgaagtgcc acgagttcgg tgctcttgag gtccccctga
     3541 cggcaagtcg gtgagacctc cggtcgctcc acttctctcc gacgatagag cgcaatccgc
     3601 ttgcggcgtt cggtaatggc ggcggacagg ccggcgtctc cctgcgtgag cctctcggcg
     3661 tccaggaggg gtagttcggc atgctcgagg tacaacgacc gcgagggtcg gcaggtcagg
     3721 gctctgagcc aaaagtccgc cgcctcgata aaaagctcgg ccgccgctcg ctcatattcc
     3781 gcgcggtcag caggatcgac caactcggtg aggtcccggg tccggatcgc ccgctcctcc
     3841 gacatcgctg cggcgcgcgc cgccaggtcg gcggtgtcgt ggtctcctgt gcgacacact
     3901 cctgagacga acaatttgta atcgtctgtg gctcggtcga ggacgcgaat aattgcctta
     3961 tctttttcca gccgctcctc cgccgcagag ccggaggagg cgggtcccgc cgtagagacg
     4021 acggacaata agaagtttgc cagcatggac atcatccctt gggcttgacc caataatggt
     4081 gcttataccc agtaacgcgg ctctgcgcgg ctgcgaagct gattgcgcgc gtaatggcca
     4141 cttcgttggc ctcgatacag ttcggatcgc tgagcttgtc gtccgattcg accttcctca
     4201 cgcggcgaac agccccagtg tccacatcga tcgaaaactc cagcgtcaac atcgaggtgc
     4261 ttgacgtgac cttcgctcga cagaacatct ggatgactcc ctggaacccg acagcgacct
     4321 gatccatcgt tagcttcgcc ggggggggcg cctcggccgg aggtggtttg tgcgctggag
     4381 cgggccgctt cttgcttctg cgcacatcgt gggcgtcgac cacacccccg ggcttcgcct
     4441 tctcaacgat gtgctcgggc ttcgcaatgg tgaccacggg cgttgtggcc ggggccgtgg
     4501 gggatacacc cggctcacca gtcgttgcag ggcagtacgg agtcgtgctg gcggccccgg
     4561 cgggcacttc ggacgggaaa gccgctcttg cgacttccgc cgggcaatcc ctggtcgctg
     4621 acacgagtgc cgtcgctaca tcttttaagg cgagtagcac gagcgccacc gcgaacgcgc
     4681 ccccgagcgg cagggcgagg cggtgtgcca gcgtgcgtgg cggcgtgaag aggccgccga
     4741 ccctcgactg gacgtccttg taatccgggt tgacggccag gacgtggccg aagctcgcca
     4801 gcgcttgcgg cctgcgtccc tcggacgcgg cggcgcagcc ggagaggtag tacgccagca
     4861 agctacgggc gcgctcgatc tcctgacgct cgctgtccag ctccgggccc atcaccgagc
     4921 gcaacgcatg ctgctgcgag tcgagcagct cgaccgcctc gtcgaacttc tccccggagg
     4981 cgagctcgcg gacccgcgac agcgcctgct ctccctcgat acgaacgatc tcgagatcga
     5041 cctccgagac cagcgtcggc tgctcggccc tggcatagag gtcgcgcgcc tcggcgagcc
     5101 tcccggccgc gtgcgcgcgc cggccctgct cacgccagat gttgcgcatg ccggtgatcg
     5161 cctcggacgt tccaggcgcg acctccagga tcatctggaa gcattgcagg cagctggcct
     5221 cctgctcctc gtcgcccgcg aggttctccg ccttggtcag gagcagctgc acgtaacgtg
     5281 ggcgaagcgc ggcggtctgt agcggcagca ggcgggcgat gacctggatc gcgcggtcga
     5341 ggctgccttg cgagcggtag acctcggcga ggagctcggt cgccccgaca tggttcgggt
     5401 ttcctttgcc ctcattgagc acgagcccga ggcgttgaat gatgagatcg atgtccgcgg
     5461 gcgagcgctc gccggtgttc cactcctgaa gcgcccggcg atagtcggcg tcggcctccg
     5521 ggttgacctg atcgatgtac tcgcgcacgc tggcgaatgg gcgatacttc tcgacccaca
     5581 tgcgcagcag ctcgaccttg aagcgatagc gccgcggcga tgtctgtgtc tcgtcgagga
     5641 cgtcccagtc gcgcagccgc gcgggcgcct ctgcgagctg gcccatcatg atgcgcaccc
     5701 cgctctgcag cagcagcttc tcgagctcct ccgcggtcag cgaccgcgag ccatgctcgg
     5761 cgaacgcgga ggcgacgacc cggcacgcgg gcgtgaggcc ctcccacagc cagctgagga
     5821 tattgtggct ctcgcgcagg acgctgtggg tgagctcctc gagctggcga atatcgacgc
     5881 ggggctcttt gctcagcatc gcgctctccc acgcgcgcga cgcgagcagc tgggtcagca
     5941 tcgggtggcc ggcggtgagc cgccagatcg cgtcgatgac cccgtgtccc cactcgatcc
     6001 cccccgtgtg ggtccctcgc gcgaggagca ggtcgaagtc ctggcgcccg agcgtcgaca
     6061 ccggctcggt gcgcaggcca cgaaacagcg gcccggtgga gatatcgagg tcttccatcg
     6121 tcctgcccat cgcgtagatc gcggcgacgc gagtgcgcag ggtgttgtcg tcgaagatgc
     6181 tgcgcaggaa ctcgaacagg cgcttgctgg cggagttcgt cttggggtcg gtcagcacgt
     6241 cgaactcgtc gagaagcaac acgaggcgtg agccgtctgg acgggccttg agcacttcgg
     6301 gcagccatgt cttgcagaac gcgcccatga tgtcctcgcc gacgaagacc ggatcgggga
     6361 gccccagcgc cgttgcgatg atcgaggcga gatcctcgag gatctcctcg atcgtcgccc
     6421 gggtgtgccg ctggagatcg aagaacaccg gcttccaggg gccgagcttc gggagctgct
     6481 cctccagttc tcgcaagatc gagctcttcc cgatccgtcg ctggccaaat agcaccagac
     6541 cgggctcgag cggacgtctg agcacgctca cgaccttgtg aatgatgtcg ctgcgcccga
     6601 cgaagtaagg cgtcccaccg accgcgttgc cgaccgcgaa gggattgctg ggaggatcga
     6661 tcactgacat tgcgcctcca tgaggggcgc ggcccctcga cccaggagcc cctccattcg
     6721 ctgcgccgtg gccaccagcg cctccgcttc gcccgaggag acgtgacccg ccagtccgtg
     6781 ttgcgcgagc cactggcgaa gatcagtgcc cgcggcgatc gcgtgcagct gatcgaggat
     6841 ccaccagtac aacagcggcg gtgtgatctc caatccgggc gccgcgcggg tctccgccag
     6901 gatgccgcgg gggatcaggc gatcacaggt ctcgaccgtc ggctgccacg gggcgccgct
     6961 cgtgaacggt gcgccccaca catgcgcgtc gcctcgcccg ccgtgcccgg gatgctcgag
     7021 ctcgaggcgc tttctcaaga acgacgggac cgcgccgagg cggtcgagca ggagggccgc
     7081 cgcggtcgag taggcgaggt cgctgaccga atctcccggc aacgcggcga ggtcgcgcgc
     7141 aggcaagacg tgcagcagga gccgacgcaa ggagtcgcgg tcgaagctct ccaggagccg
     7201 gatcaatgcg cccggagcga gcggctcgtc ctgtatctcg cgcagcggcg caccggggcg
     7261 ggagaacgcg accgcggacc gctgtgcgag cgccgtgcgc agcaagatcc agcgctcttg
     7321 ccagggaagg aacagccacg cgttctgcaa gacgacgcga agctgctgac ggcagccggc
     7381 gatcgcctgc aggcgcgctc cagaccccct gttcccggct gccaggcttt gttcgagggc
     7441 gtcgccgaga aagcgcagca tcttggggtg gccgccggtg cagcgcagga tccactcgtg
     7501 atcttcgggc gtcaggtagg ggcggtggtg gccgaggtag gccagcgcgt cgctgcgggt
     7561 gaacggtccg agcaccagct cgcgcatgcc gttgaagaac ggggagccat acatccattc
     7621 ctgcgtcgcc gtcgtgagcg cgacgaggtc gatgcgactg gtggcgacga gggtgaatcg
     7681 cggcgtctgc gtcagtgatc gcagcttgcc ccacgggtcg ccccggcgaa aatctggcag
     7741 gtgcaccagg gcctgaaggt cccggatcag caagatatgt cgcttgcccg gctcggcgag
     7801 tagtcgctcg atgccgcgaa agctcagatt gccgccgcga gcgccgccga actgcccgag
     7861 caccgagctc cagaacctgt ccgcagtggt gctggaatcg agggtctgga catcaataaa
     7921 ggcgacccgg caatctctcg cctgaagtcg ctgttgcagc gcgcgcagca gagtggtccg
     7981 cccactcggc aactcggaga cgattgccgt gctctctccg gtatggacgc tgcgatcctc
     8041 aaggatcgca agttcggacg tgcgagttaa aaatgctgtg tccatacgct cgcgtgctgt
     8101 tgatcgggat cgtcggactg gcgagcagcg agcaggcata tgtcgccgtc cgacccgccg
     8161 agatggtctg ctcggggcag cgtgccacat tccatgggcc ttgagcgact tcgcgcatca
     8221 cccggattta ctgtgtttcg cgttggaatc gtggattcac gacgcagggt tcatggtcgc
     8281 aaagggggcg ccgtctgacc tgcacggtcg gtctggtgtg ccccctgcgg cccaattgat
     8341 cagtcccgcc tcgcgcagcc ttcgtcgttg cgagtgagat atatccggtg cccgcaggag
     8401 tctcgccgag atggcgcgga cgcggcgccg tcagtattcg acgcggacgc cgaagacggg
     8461 gaggattggg acaccgaccg tggtcttcac gacggtaaag tgcgggttgt agatcaggtc
     8521 ctccgggttt tgccgattat aaatgttctg gacgtcgaag taggcgttga cgacgctgcg
     8581 gcgcagcacc acgagcgatc gacgcgcagg tcgagctggt gaaaggtcgg catccgggcg
     8641 ctgttggtgg cgccgaacag ggggtcgacg ggaacgcaga gaactggccg ggcagatgat
     8701 cggcgctgta gaccccgcca aggatcggcg tataaggcag cccggtgagg tcctgcccat
     8761 acccccctgc acggaccacg ctggctgaga tgccccacgt gatcgggtag ctgacgaccc
     8821 actccggcgg gtccggtggg atgcaggcgg cgaggcacgc gagcgcgagc caggccgggc
     8881 gcatgggggt ctgcatgcag acccagggta tcggagatcg ccgggagccg gtcagggatt
     8941 ttatctggat gtcccggggg acaggtccgg agacctgccc ccgggcggcg cgtcagaagc
     9001 cgcagcaggt cttgcccttg gcctcgaggg cgtcgaagtc catgcaggcc tcgtcggcct
     9061 ggcactcgcc gaccttctcg tcgccgacgg tgcaggtgca ggtctcgccg gtgcacagca
     9121 tcttcttggt cggctcctcc tcgcactcga tcgtcaggtc gcactcgtcg ccgaaggcgc
     9181 cggtgaggac gctgcagccc tcctcctcgc tgccctggca ggcctgggcg acggcttcct
     9241 cctgctccgc gcaggcctcg gccgggccct cgagcagctg ctcgcaggtg agcgcggtgg
     9301 cgcaggcgag catgtccttg ttggcgtcct tgcaggcgcc ctcgtagaag ccgaggtcct
     9361 cgacgcagcc ggcggtgcat tcggcctcgt cctgcgggaa gccgcactcg gtccagcgcg
     9421 cgcagtaggc ctcacagccg ctcacgatct cggggtcgac cgggtcggtg gtctcgccct
     9481 cggtggtcgc gtcggtggtc gcgtcggtgg tcgcgtcggt gtcggacgta tcgtcgccgg
     9541 cgctgtcgtt cggcttggag gtgacgccgg tggtcggcac gtcgccggtg gtgtcgccgg
     9601 tcgcggagtc ggagccggcg gtgtcggtcg tggcgtcggt gtcggaggtg gtgtcaggcg
     9661 tggtgccggt gctgtcggcg gcgccggtgc tgtcggcgtc ggtgtcgctc gtgttcgagc
     9721 cgttgtcgcc gcaggcggtg aagaggagga gagcaagggt ggtgaagcca agtcgcgtat
     9781 acatggttgc ctttcgcggg gcgccggaga tcgatccctc gacgacgcct cggtcggacc
     9841 tgtgagctgc gagacccggc gaaacggatc acgaaaaaaa cgatgtaggg tggcgcatgg
     9901 gggctctggc ggcgatgtcg tgagggggct caaggctgtc tctcgggaca tgtcaggcgg
     9961 cgagctcgtt cgggatcgcg aggggctcgc gcgcgtcggc gatcagcacg gcgagcgggt
    10021 ggccggggcg gtgccagtac agcgcgggcg acacggggtc cgcgaggcgg gcgtatgcat
    10081 tggcggtcca ggtcgcgagg aagcgcagcg agtcgcggac gtgcaccggc gcgtcgtcgc
    10141 cgtcgctcga ccagaactga cggaagcggc cgatgcgggc gagcacgcgg cacaggccgc
    10201 gcgcgtgcgg ggcgaggaac agcgcggtgc cgcgggccgg cacggcgagc acgatgcccg
    10261 cggcgccagg gtcgatgtgg gccgcgagct cgagcgcggc gacggcggtg tcgcgtccgc
    10321 ccgggccgcc gaggatgtac gcgtgcacgc ccggcagcgg gccggcgcgc ccgaactcca
    10381 gcggctcgcc gcaggtgccg aggcgagcgc gcgtgaaggc ggcgtcgggg tcgaggccgt
    10441 ggaagcgcag cacgccgcgg gcgggcagct cgagggtgcc gtcgatgcgg cgcagggcga
    10501 ccagctcgtg caggccgggg gcgagctcgc gggcctcggc gtgggggtgg cgcgcgcgtt
    10561 cggcggcgca gaccagcaga gatcgcaggc gcgggccggg gagcgggagc ggggcgggag
    10621 cggcggtggt ggcggacagg gcgggatgcg cgagagcagt ggacatgcgg gggcctcgcg
    10681 ccttcatcga catgccgggc gcttttgttg cgcggggcgt cagaagtttt gttggcgggc
    10741 ctccggctcg tgccggcggg gggcagcggt tatgctggga gggatgtccc gcgacctgct
    10801 gctgttgctc ggcctcgtgg ccctgaccgg ttgtgccggt gaggtcacgg cgttcgcggc
    10861 gatggtgttc agcatgagcc tgggcgcgtg gctggtcggc cgcgggcacc agcggcgggc
    10921 gctgctcgag gccgtgcagc tgcggcgagc gatcgccacc gggcggcacc gggagatcga
    10981 gcagcacctg cgccgcgagc tggcgctggc ggccgcgggc gaggcggtga gcgtcgagcg
    11041 gcagtggctc gcgcgggccc agctcggcgg tttgctggtc gccgagtggc ggctcgacga
    11101 ggcgcgggag atctacacga ccaccacggc caagctgtcg ccgcacctgc aggcgctcgc
    11161 ggccttcggg cggcacgagc tcgatgtcct gacccgcgag ccggacacgg tgatcctcga
    11221 ctgcatccgc agcgaccgcg acgcgtgcct gcggcacatc ccgaccccgt atcagtcgac
    11281 cgtcgcgcgc acctgggccg cgctcgaggg gctgtgcctg gcccgcatgg gccgcccgcg
    11341 cgaggccgtg cccttgctcg cgcgtgggct cgagtgcctc ggctacaacc cggcgcgcgt
    11401 cgtctacctg tttcacctcg cgcaggcgca cgagcagctc ggcgaccgac agctggcggc
    11461 gtcgcgctac gagcaggcga tgcacgcgtt cccgggcacg cggctggcca gcgaggcccg
    11521 cgcccgctgt cacgcgctcg acgcgggccg tggtgaccac ctgttccgcg ggatgttgcc
    11581 cgagccgccg atggctgcgc gcgagcgcgg ctaggcggcc gcagctgacc gatcggacag
    11641 ctcgcgcgga cggctgtcca tcgctcccga tctccgcgat cgagatctat tcgtcgatga
    11701 actaccgcgg cgggccagtg ctggtagaat gatcgtccgt gagcgaaggc ttcgtgcgca
    11761 aacgcctgca cagcgcggcg gagacgatgt cccacagcct gggatacgcg cgtcacctgt
    11821 tgcgcgggaa ccgggtcgcc ggcgacctgt cgtgggtgca gccgggcacg cggccggtgg
    11881 tgctgttgca cgggtttctc ggcacgcgag ggacgatgca gccgctgacg cggcgcttcc
    11941 aggccgacgg ccgcgtggtc ttcagttacc accacggcac cttccagctc ggctcgctgc
    12001 gatcctcctc ggaggagttg atccatcgcc tgcgcgacct ggtcgagaac ctcgagctcg
    12061 accgcgtcga cgtgctcggc ttcagcatgg gtggcctggt ggcgctgcac gcggtgaagt
    12121 tcctgcaggg ccagaagtgg atccgccggc tggtgctgct cggcacgccg ctcgcgggct
    12181 cgtggatgtc gctcgcgggg atcgccaccg tcgggctcgt cagcccgtcg gtgtggcagg
    12241 tgcggccggg ctgccagttc ctcgacgagc tgcgcgccgc accgctgccg cacggggtgc
    12301 gcgtgcgcca gatccacgcc gacgccgacg ccttctgtcg caaccccgga cccgtcgagg
    12361 gcgtcgaccc cgagcacgac tacctggtcc tgcccggcgg ccactcctcg ctggtcatcg
    12421 cgcagcactt ctatgccgcg gcgcgcgagt tcctcgacga gaccgacgag gccgagatcg
    12481 aagtgcggcg ctgaggctgc gagctcagtc gccgatgcgc gccttgacct tgtcgatgcg
    12541 gccctgggcg ctctcctcac cgagctcctg ggccttctcg tagtgcttga gggcgtcgcg
    12601 gtagcgcagc acattataat aggcgtcgcc gagcttgagg ttggaggcgc gcgactgcgg
    12661 ggcggcctcg acggcgagct cggcgaactg cacggccttc accttggcgc cggtgtcgaa
    12721 gtacacgtcg ctgaggccca tcagcgcctt ggcgttgcgg ttgtcgaacg agatcgcctg
    12781 gtggaacagg ttctcggcct cgctgcgatt gccggcgcgt agcgccttgg cgccctcgtc
    12841 ggcgagcttg cgcgcgcgcg ccgggtcgcg ttgattgcga gcgaggaccg gcttgccggg
    12901 cttggcgggg cggtcgggag cggcgtcggg ggtgtcctcg aggacaggtg gaacgatcgc
    12961 gacgtcgggc tcgggcgccg gctcggcagc cgccacgcgg gcgcgctcgg gcgggcgcag
    13021 gccggcgccg cgcaggccct cgtcgacggt cagcaggtcg cggatcgaca tctcggcggt
    13081 ggcgagcagg cgatcgacgc gggcctgacg gcgctgctcg tcctcgtcgg cgagggcgcc
    13141 gacgaggccc atcgcctgca gctcgccgag gttccagccg ccgctcgtc
//
LOCUS       DASWNJ010000857         3326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_368208, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000857 DASWNJ010000000
VERSION     DASWNJ010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368208"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2267)
                     /locus_tag="VGB85_14600"
     CDS             complement(<1..2267)
                     /locus_tag="VGB85_14600"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0135310.1"
                     /translation="MLPFVGAGVSRSVCDRAGSPRFPTWTELLERAVHRLIEEHKDEA
                     AQAVRVQLAAATPDLIAAAETAREALWGRRWRDFLLEQFNPEIGDIDRRTLALAEAIW
                     SLECPLIVTTNYDRVLEWASPRPADCESWPTHAPAEHARALEGGPSKPIVWHLHGTIQ
                     QLDGIILTTSDYKRLYPTPSEREQYTSALLTLRNLLAIKTFVFFGFGFNDESFLSEVL
                     AVTEAFRGNVGPHYAFVHRAEIPRLAGRARELSLELLPFEAFGEPLVTCVRELGQSRT
                     RPMPPATIPPAIPEPEPASEVGIPGMAAVPGLLGDRYRLERRLGHGGFATVWQAFDKR
                     TRTRVAIKILHPHLGHDAQRVARFERGAERMGALHHRNIVRVLAPVVAEGSYRYFVME
                     LLGADLGVRSQEHRLSAPEIVSIVRQLGDALSHAHQRGLIHRDVKPANVLLDAEGVPK
                     LTDFDLVLTRDSSGGTRTGAMGSFLFAAPEVISDPGSADETADVFGLAMTAVYLFHAA
                     PMGAEAYRNATDFIDTQLQCSGAIKAVLARACSWKRERRPATVAEFCEELQAAASAPP
                     ERAPSVVVARTPAAAPRPSPPPAAAPHAPASSDGVRAAAPAAKLAAAPGRRVHLGWAA
                     LVPVAALAIAVWQLPTFLRTPALDEVSPAPSPESPVVPEEPAPSPAPAPVPTPVPTPS
                     VEDDAEARARAAALQRLRLPPRGSDRGVTRVALGDVSPIRFVELTGGEYTIGSPSSEP
                     SRQINETQHRVRVEP"
     gene            complement(2532..2930)
                     /locus_tag="VGB85_14605"
     CDS             complement(2532..2930)
                     /locus_tag="VGB85_14605"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY0135311.1"
                     /translation="MSDTDLAGGCLCGAVRLALSGAPYRVGICHCMDCRKHHGAVFYA
                     AAIFPVDAVTIRGETAHYGGRHFCPTCGSSLFARSDDEIEVYLGCLDAPNQFRPTYEN
                     WVIRREDWLPPFDVARHYQKDREGTGRSEP"
     gene            3147..>3326
                     /locus_tag="VGB85_14610"
     CDS             3147..>3326
                     /locus_tag="VGB85_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135312.1"
                     /translation="MPRTQLDLFADLRPGVAAPSPSAGGWPATPRGEPQLGLYDARSL
                     LLQRARDAFARGSLDE"
BASE COUNT          493 a         1147 c         1229 g          457 t
ORIGIN      
        1 aacggctcga ccctgacacg gtgctgggtc tcgttgatct ggcgcgaggg ctcgctgctg
       61 gggctgccga tcgtatattc gccgccggtc aattcgacga agcggatcgg cgagacgtcg
      121 ccgagcgcca ccctcgtgac gccgcgatcg gagcccctgg gcgggagacg cagccgttgc
      181 aaggcagccg cacgcgccct cgcctcggcg tcgtcctcca cgctcggtgt cggtacaggc
      241 gtgggcaccg gagcgggcgc cgggctcggc gcgggctcct cggggacgac cggcgactcg
      301 ggcgacggcg cgggcgaaac ctcgtcgagc gccggcgtgc gcaggaatgt cggcagttgc
      361 cagacggcga tcgcgagggc cgcgaccggc accagcgcgg cccagccgag gtgaacgcgg
      421 cgccccgggg cagcggcgag cttggcggcg ggcgcggccg ctcgcacgcc atccgagctg
      481 gcgggtgcat gcggggcggc cgcgggaggg ggcgaggggc gaggggcagc ggcgggcgta
      541 cgcgcgacga cgaccgacgg cgcgcgctcc ggtggtgcgc tggcggccgc ttgcagctcc
      601 tcgcagaact cggccacggt ggcgggccgg cgctcgcgct tccagctgca ggcgcgggcc
      661 agcacggcct tgatcgcgcc gctgcactgc agctgcgtgt cgatgaagtc ggtcgcgttg
      721 cgatacgcct cggcgcccat cggcgcggcg tggaacaggt acaccgcggt catcgccagg
      781 ccgaacacgt ccgcggtctc gtccgcgctg ccgggatcgg agatgacctc gggcgcggcg
      841 aacaggaacg agcccatcgc cccggtgcgg gtcccacccg acgagtcgcg ggtgagcacg
      901 agatcgaagt cggtcagctt cggcacgccc tcggcgtcga gcaggacgtt ggccggcttc
      961 acgtcgcgat ggatcaggcc gcgttgatgc gcgtggctga gcgcatcgcc gagctggcgc
     1021 acgatcgata cgatctcggg cgcggacagc cgatgctcct gcgacctcac ccccaggtcg
     1081 gcgccgagca gctccatgac gaaatatcga taggacccct cggcgacgac cggcgcgagc
     1141 acccgcacga tgtttcggtg gtgcagcgca cccatgcgct cggcgccacg ctcgaacctg
     1201 gcgacccgct gcgcgtcgtg gccgaggtgc ggatgcaaga tcttgatggc gacccgcgtc
     1261 cgggtccgct tgtcgaacgc ctgccagacc gtggcaaaac cgccgtggcc gagccgccgc
     1321 tcgagcctgt agcggtcgcc cagcaggccc gggaccgccg ccatcccggg gatgccgacc
     1381 tcgctcgcgg gctccggctc cgggatcgcg ggggggatcg tcgctggcgg catcggccgc
     1441 gtacgcgact ggccgagctc gcggacgcag gtgacgagcg gctcgccgaa cgcctcgaac
     1501 ggcagcagct cgaggctcag ctcgcgggcg cgaccggcga gccgggggat ctcggcgcgg
     1561 tgcacgaacg cgtagtgcgg gccgacgttg ccgcgaaagg cctcggtgac ggcgaggacc
     1621 tccgacagga agctctcgtc gttgaacccg aagccgaaga atacaaaggt ctttatcgcc
     1681 aggagattgc gcagagtaag gagcgcgctg gtatattgct cgcgctccga gggcgtcgga
     1741 tacaggcgct tataatcgct ggtcgtgagg atgataccgt cgagctgctg gatcgtgccg
     1801 tgcaaatgcc agacgatcgg cttgctcggg ccgccctcga gcgcgcgcgc gtgctcggcg
     1861 ggagcgtggg tcggccagct ctcgcagtcg gcgggcctcg ggctcgccca ctcgagcaca
     1921 cgatcataat tcgtggtcac gatgagcgga cattcgagcg accatatcgc ctcggcgagg
     1981 gcgagggtcc tccggtcgat gtctccgatc tccgggttga actgctcgag caggaaatct
     2041 cgccagcgtc gcccccacag cgcctcccgt gcggtctcgg ccgccgcgat caggtccggc
     2101 gtcgcggcgg cgagctggac gcggaccgcc tgcgccgcct cgtccttgtg ctcctcgatc
     2161 agccgatgca cggcgcgctc gagcagctcg gtccacgtgg ggaacctcgg cgagcccgct
     2221 cgatcgcaga ccgacctgga cacgcctgcg ccgacgaagg gcaacacgcg cccttctctc
     2281 agcgccagca tgagggagcg cggaaacttg cgcatgcacg aggatgccgg tcggcccgcg
     2341 gtgaagtcaa tggccgcgca gccgcggggc tccccaggaa caatgttcac tcgccgcagg
     2401 gccgtcccgc gcatgttgct gacgcagcgc acgcggccgg atgaccgggt ccggggcggc
     2461 tggcagcggc ccgggtccga agccgccggc ggcacgacga tgtcccgatc ggatgcctgg
     2521 cgtccactgt ttcacggctc cgagcggccc gtgccttccc ggtccttctg ataatgccgc
     2581 gcgacgtcga agggcggcag ccagtcttcg cgccggatga cccagttctc gtaggtcggc
     2641 ctgaactggt tcggtgcatc gaggcagccg aggtacacct cgatctcgtc gtcgctgcgc
     2701 gcgaacagcg aggatccgca cgtgggacaa aagtgccggc ccccgtaatg cgcggtctcg
     2761 ccgcggatcg tgaccgcatc gaccgggaag atcgcagcgg catagaacac cgcgccgtgg
     2821 tgcttgcggc aatccatgca atgacaaata ccgacgcggt acggcgcacc cgagagggcg
     2881 aggcgcaccg cgccgcacag gcagccgccg gcaagatccg tgtcgctcat ggttgtctcc
     2941 ctgaaaatgt gtgtagctcg cggcgcgcga gcgcggggtg gttttagcac cagaccggag
     3001 ggcgacccgg cggcggacat cgccgccgcg gcgaacgtcg agatggccct cgcggggccg
     3061 catccgcgag cgccggcgat gagcgcgggc cccaatgcgg gcagggccgc gggggcaggg
     3121 tcgggcacct cgtggtaaag gtcgacatgc cccggacgca gctcgacctg ttcgccgacc
     3181 tgcgcccggg cgtcgccgcg ccgtcgccgt cggcgggcgg gtggccagcg acgccccggg
     3241 gcgagccgca gctcggcctg tacgacgcgc gcagcctgct gctgcagcgc gcccgcgacg
     3301 cgttcgcccg tggatcgctc gacgag
//
LOCUS       DASWNJ010000858         1728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_85229, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000858 DASWNJ010000000
VERSION     DASWNJ010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85229"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="VGB85_14615"
     CDS             complement(<1..1088)
                     /locus_tag="VGB85_14615"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HEY0135313.1"
                     /translation="MRVLFVASEVFPLVKTGGLADVVAALPPALEQLGVDVRVLVPGY
                     PKVMQGLEATGREVKLGGLLGGHSARLVYGRVGNLQLLVLDAPHLYDRPGNPYTGPDG
                     RDWPDSHLRFAALCDAAAWLAGPEGGERWRPDVIHGHDWQSGLAAAYSKLGPGPRPGT
                     VLTIHNLAYQGVFSKEVLPELRLPWSMFDIHGLEYHGHLGFLKAGLWYSDKITTVSPT
                     YARQIQTPTYGHGLHGLLQRRDADLHGILNGIDTELWNPATDRHLAAHYSAQDPFAGK
                     RANKLAAQRAFGLEQNPDVPLFCVVSRLIAMKGLDLLISAGTTLLRRGAQLAVLGSGE
                     QAIEQGFDMAAARFPGRVAVRHGYDEPLA"
     gene            complement(1098..>1728)
                     /locus_tag="VGB85_14620"
     CDS             complement(1098..>1728)
                     /locus_tag="VGB85_14620"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY0135314.1"
                     /translation="NGDFLFDQLLRDSEDEQSSHDFGKDILPYLVRQRAAIYAHGFPE
                     SCVKTKPDAPTYWRDVGTIDAYWEANMDLTSVLPPLDLYDPKWRIFTSQEQLPPAKFV
                     HDDDTRRGSAVNSIVSAGCIISGAEVRGSLLSSNVRCNSYSTLCEAVVLPDVVINRKA
                     RLKKVVIDRSVIIPEGLVVGEDPVLDTKRFYRTESGICLITQDMLNRLK"
BASE COUNT          258 a          529 c          672 g          269 t
ORIGIN      
        1 gcgagcggct cgtcgtagcc gtgacgcacg gcgacgcggc cggggaagcg cgcggcggcc
       61 atgtcgaagc cctgctcgat cgcctgctca ccggagccga gcacggccag ctgggcgccg
      121 cggcgcagca gggtggtgcc ggccgagatc agcaggtcga ggcccttcat ggcgatcagg
      181 cggctgacga cgcagaacag cgggacgtcc gggttctgct cgaggccgaa ggcccgctgc
      241 gccgcgagct tgttggcgcg cttgcccgcg aacgggtcct gcgcgctgta gtgggcggcg
      301 aggtgccggt cggtggcggg gttccacagc tcggtgtcga tgccgttgag gatcccgtgg
      361 aggtcggcgt cgcggcgctg cagcaggccg tgcagaccgt ggccgtaggt cggcgtctgg
      421 atctggcgcg cgtaggtcgg gctgaccgtc gtgatcttgt cgctgtacca caggccggct
      481 ttgaggaagc cgaggtggcc gtggtactcg aggccgtgga tgtcgaacat gctccacggc
      541 aggcgcagct cgggcagcac ctccttcgag aacacgccct gatacgcgag gttgtggatc
      601 gtcagcacgg tgccggggcg cgggcccggt cctaacttgc tatacgccgc ggcgagaccg
      661 gactgccagt cgtggccgtg gatgacgtcg ggccgccagc gctcaccacc ctcgggcccg
      721 gcgagccagg ccgccgcgtc gcacagggcc gcgaagcgca ggtggctgtc gggccagtcg
      781 cggccgtcgg ggccggtgta cgggttcccc gggcggtcgt agaggtgcgg cgcgtcgagc
      841 acgagcaatt gcaagttgcc gacgcggccg tacacgaggc gcgccgaatg gccgccgagc
      901 aggccgccga gcttgacctc gcggccggtg gcctcgaggc cttgcatgac cttggggtag
      961 ccgggcacga gcacgcgcac gtcgacgccg agctgctcga gcgcgggcgg gagggcggcg
     1021 acgacatcgg cgaggcctcc ggtcttgacc aggggaaaaa cttcggaggc gacgaagagt
     1081 acgcgcatgg ggagggtcta cttcagccgg ttgagcatgt cctgcgtgat caggcagatg
     1141 ccgctctcgg tgcgataaaa ccgcttcgtg tcgagcacgg ggtcctcacc gacgacgagg
     1201 ccctcgggga tgatgacgct gcggtcgatg acgaccttct tgaggcgggc cttgcgattg
     1261 atgacgacgt ccgggaggac gaccgcctcg cacagcgtgc tgtaggagtt gcagcgcacg
     1321 ttggaggaca gcaaggagcc gcggacctcg gcgccggaga tgatgcagcc ggcggacacg
     1381 atcgagttga cggcggagcc gcggcgggtg tcgtcgtcgt gcacgaactt ggccggcggg
     1441 agctgctcct gcgaggtgaa gatccgccac ttggggtcgt agaggtcgag cggcggcagc
     1501 acgctggtca ggtccatgtt ggcctcccag taggcgtcga tcgtgccgac gtcgcgccag
     1561 taggtcggcg cgtcgggctt ggtcttgacg cagctctcgg ggaagccgtg cgcgtagatc
     1621 gcggcgcgct ggcggacgag atacggcaag atgtccttgc cgaagtcgtg gctgctctgc
     1681 tcgtcctcgc tgtcgcgcag cagctggtcg aacaaaaagt cgccgttg
//
LOCUS       DASWNJ010000859         4392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_686080, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000859 DASWNJ010000000
VERSION     DASWNJ010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686080"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VGB85_14625"
     CDS             complement(<1..402)
                     /locus_tag="VGB85_14625"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEY0135315.1"
                     /translation="MSRRRRPHRDVVGAVGIWCFALQGLVLSLLLTALKFRADSLCDD
                     DILGACPLAATSSCATVLTDAWSVVLGAPLTVHSAAFYLVIAGLAALTVTGSALRRFT
                     RPLLLVGAWLGLLVTLAFAARAGLWLRALCPY"
     gene            complement(614..2269)
                     /locus_tag="VGB85_14630"
     CDS             complement(614..2269)
                     /locus_tag="VGB85_14630"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0135316.1"
                     /translation="MQQVLIVDDNPSVCTALEILFDVHGIPTIIANTPEAALEVVARG
                     DVGAVIQDMNFSDDTTSGQEGAALFRRLRELDGDLPVLLMTAWTSLETAVLLVKEGAA
                     DYLAKPWDDAKVVTTVKNMLRLRELALENHRLRAQSARARNELQDRYDLCGLVYASEA
                     MHRVVSLAASVARADAPVLISGPNGSGKEKVAEIVHANSPRRQAPFVKVNAGGLPDDL
                     LEAELFGAEVGAFTGATKRRIGRFEAADGGTLFLDEIGNLTLRGQVKLLRVLQTGEFE
                     RLGSTVTRKVDVRIVSATNANLRQMIAAGSFREDLLFRLNVIEIRISPLRERSEDVLP
                     LAEHFLHTLGQAARLGEALDSRNDSRNDSRVTGASTSTTSGAVTTTSGVVPSTTSASI
                     STTSGVVSGVASTTGVGPTTLTASTSPVNAAPPTRFSPAAVRSMLGHDWPGNVRELQN
                     RIQRAILLCHGGVVEPGDLGLDDASIAADTVRPRQHNASEPSSRVIGNEDTETERRQI
                     VAALEAHAGVVARAAAELGLSRQALYRRMDRLGISLERQLRQS"
     gene            2514..3428
                     /locus_tag="VGB85_14635"
     CDS             2514..3428
                     /locus_tag="VGB85_14635"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0135317.1"
                     /translation="MEDLFHRGNVSASGLQFSTLEGSRPRPGEARPVVLCLHGFPDHN
                     RSFRHQIPALTEAGYRVVAPMLRGYEPSSQPIDDDYHVIRMVEDVLGWIEYLGVLRVH
                     LVGHDWGAVIGYQVAAHAPERLLSLTTIALPHLRRIASGIMQHPQQLLNSWYTMFFQL
                     RGVAELALRRNDFALIEKLWRDWSPGWDPPEEEMRLLKRTFRQPGVLKAAVRYYRAAF
                     DPITRAAAQTRKLAFSTIPVPTLAITGERDGCVDTRLFDDAMRGADFSRGLKIRRVRN
                     AGHFVHQEQPEEVNTLLIEWLRKHDRSA"
     gene            3425..4387
                     /locus_tag="VGB85_14640"
     CDS             3425..4387
                     /locus_tag="VGB85_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135318.1"
                     /translation="MSVRAAWMLAIGLGCAPAGSGPIAEPAPAAAPEPATAPELPPEL
                     RGVVAPWQRVEPDWLARASVWAVANYVEHRVHQQRGAPGHTAWILLDAFVPTSVPRGD
                     FTAPELLIDRQALQGGEYPPFYAEGRSYLVLLRPGAETARRLADPAGRFTMYERLGPD
                     EVVAIVDLSQSEAEARVAAIAASRSGEREGVRFDPQRWAEARDAPAITPGLHAPVSRF
                     LAAQLKIGMPIDEVRGWLGVPDEQRLVGGTRIDDYLLARAQYRSAHDSATYGVLELRH
                     VDGRLTEGQLRYFRWRVSPGEASSHELSAEQLRALGVASHVLAP"
BASE COUNT          603 a         1445 c         1718 g          626 t
ORIGIN      
        1 gtaagggcac agcgcgcgca gccacaggcc ggcccgcgcg gcgaaggcga gggtgacgag
       61 caggccgagc caggcgccga cgagcagcag cggccgggtg aagcgacgca gggccgagcc
      121 ggtgacggtc agcgcggcga ggccggcgat cacgaggtag aaggcggcgc tgtggacggt
      181 cagcggggcg ccgagcacga cggaccaggc gtcggtgagc acggtcgcgc acgaggaggt
      241 cgccgcgagc ggacaggcgc cgaggatgtc gtcgtcacac agcgagtcgg cgcggaactt
      301 gagggccgtc aacagcaggc tgagcaccag gccctgcagc gcgaagcacc agatcccgac
      361 ggcaccgacg acgtcgcggt gcggacgtcg gcgccggctc acgtcgcggg ctccgtcggg
      421 gaggtcgggg acacgcgcgc gattatagcc gtccgcgtcg cgggctgcgc cgggagttgc
      481 cgtgtgctgc gctgcgtcca tcgccgggcc acggccgggc gtggccgggt ggtacggggg
      541 ttcggctcca ggggggcagg tgttgcgccc cgaagtggtt gcgcccgaag tggttgcgcc
      601 cgaagtggtt gcgtcaggac tgtcggagct ggcgctcgag gctgatgccg aggcggtcca
      661 tgcggcggta cagggcctgg cggctgaggc cgagctcggc ggcggcgcgg gcaacgacgc
      721 cggcgtgggc ctcgagcgcc gcgacgatct ggcgccgctc ggtctccgtg tcctcgttgc
      781 cgatcacgcg ggagctcggc tccgaggcgt tgtgctggcg cgggcgcacg gtgtcggcgg
      841 cgatgctggc gtcgtcgagg ccgaggtcgc cgggctcgac gacgccgccg tgacacagca
      901 agatcgcccg ctggatgcgg ttctgcagct cgcgcacgtt gccgggccag tcatggccga
      961 gcatcgagcg caccgcggcg gggctgaagc gggtcggcgg cgcggcgttg accggggagg
     1021 tggaggcggt cagcgtggtc gggccgacgc cggtcgtcga cgcgacgccc gagacgacac
     1081 ccgaagtggt cgagatcgag gcgctcgtgg tcgagggcac gacgccggag gtcgtggtga
     1141 cggcgccgga ggtggtcgag gtgctggcgc ccgtaacgcg gctgtcgttg cggctgtcgt
     1201 tgcggctgtc gagggcctcg ccgaggcggg cggcctggcc gagggtgtgt aaaaagtgct
     1261 cggcgagcgg caggacgtcc tcgctgcgct cgcgcagcgg cgagatgcgg atctcgatca
     1321 cgttgaggcg aaacagcagg tcctcgcgga agctgccggc ggcgatcatc tggcgcaggt
     1381 tggcgttggt cgccgagacg atgcggacat cgaccttgcg ggtgaccgtg ctgccgaggc
     1441 gctcgaactc gccggtctgc agcacgcgca gcagcttgac ctggccgcgc agggtgaggt
     1501 tgccgatctc gtcgaggaac aaggtgccgc cgtcggcggc ctcgaagcgg ccgatcctcc
     1561 gcttggtcgc gccggtgaag gcaccgacct cggcgccgaa cagctcggcc tcgagcaggt
     1621 cgtcgggcag gccgcccgcg ttgaccttga cgaagggcgc ctggcgtcgc ggcgagttgg
     1681 cgtgcacgat ctcggcgacc ttctccttgc cggatccgtt gggcccggag atcaacaccg
     1741 gggcatcggc ccgcgcgacg ctggcggcga gcgagacgac gcggtgcatc gcctcgctgg
     1801 cgtacaccag gccgcacagg tcgtagcggt cctgcagctc gttgcgggcg cgcgcgctct
     1861 gggcccgcag gcggtggttc tcgagggcga gctctcgcag gcgcagcatg ttcttgacgg
     1921 tggtgacgac cttggcgtcg tcccacggct tggcgaggta gtcggccgcg ccctccttga
     1981 cgagcaacac ggcggtctcg agcgaggtcc acgcggtcat cagcagcacg ggcaggtcgc
     2041 cgtcgagctc gcgcaggcgg cgaaacaggg cggcgccctc ctggccggag gtcgtgtcgt
     2101 cgctaaagtt catgtcttga atgacagcac cgacatcacc gcgggcgacg acctcgagcg
     2161 cggcctcggg ggtgttggcg atgatcgtgg ggatcccgtg cacgtcgaac aggatctcga
     2221 gcgccgtgca gacggagggg ttgtcgtcga cgatcaggac ctgctgcatg acctctccgt
     2281 tgtacccgca tccggggccc gcggacgaca ggtcatgacg cgccgcgcat ggtccgccca
     2341 catgtgcgca gggcggttgc acgccacgtt tcccggtcgc cggccgcgct tacgacgttc
     2401 gcgggggcga ccagtgtttt cacggcgctt gcttcgggcg caggcgagcg cggcgcaggc
     2461 gtggagggcc gtccggtcga cgccacggcc ttcggatggt agcaagggcg ggcatggagg
     2521 atctgttcca ccgcggcaac gtctcggcga gcgggttgca gttctcgacc ctggaaggct
     2581 cacggccgcg gccgggcgag gcgcgtccgg tggtgctgtg cctgcacggc ttccccgacc
     2641 ataaccggtc gtttcgccac cagatcccgg cgctcaccga ggccggttat cgcgtggtcg
     2701 cgccgatgct ccgcggctac gagccgagct cgcagccgat cgacgacgac taccacgtca
     2761 tccgcatggt cgaggacgtg ctcggctgga tcgagtacct cggggtcctg cgggtccacc
     2821 tcgtcggcca cgactggggc gcggtgatcg gctaccaggt cgcggcccac gcccccgagc
     2881 gcctgctgtc gctgacgacg atcgccctgc cgcacctgcg gcggatcgcc agcgggatca
     2941 tgcagcaccc gcagcaactg ctcaactcct ggtacacgat gttcttccag ctgcgtgggg
     3001 tcgccgagct ggcgctgcgt cgcaacgact tcgcgttgat cgagaagctg tggcgcgact
     3061 ggtcgcccgg ctgggacccg ccggaggagg agatgcggct gctcaagcgg acgtttcgcc
     3121 agccgggcgt gctcaaggcc gcggtgcgct actaccgggc cgccttcgac ccgatcaccc
     3181 gcgcggcggc gcagacccgc aagctggcgt tctcgacgat ccccgtgccg acgctggcga
     3241 tcaccggcga gcgcgacggc tgcgtcgaca cccgcctgtt cgacgacgcg atgcgcggcg
     3301 ccgacttctc gcgcgggctc aagatccgca gggtgcgtaa cgccggccac ttcgtgcatc
     3361 aggagcagcc cgaggaggtc aacacgctgt tgatcgagtg gctgcgcaag cacgaccgga
     3421 gcgcatgagc gtgcgggccg cgtggatgct ggcgatcggg ctgggctgcg cgccggccgg
     3481 gtcggggccg atcgccgagc cggcgccagc ggccgcgccg gagcccgcga cggcgccgga
     3541 gctgccgccg gagctgcgcg gggtggtcgc gccgtggcag cgcgtcgagc cggactggct
     3601 ggcgcgcgcg agcgtgtggg cggtcgcgaa ttacgtggag caccgggtcc accagcagcg
     3661 cggcgcgccc gggcacacgg cgtggatcct gctcgacgcg ttcgtgccga cgagcgtgcc
     3721 acgcggagac ttcacggcgc cggagctgtt gatagatcgc caggcgctgc aaggaggcga
     3781 atacccgccg ttttacgcgg aggggcgcag ctacctcgtg ttgctgcgac cgggcgccga
     3841 gacggcgcgg cgcctcgcgg atccggcggg ccgcttcacg atgtacgagc gcctcggacc
     3901 cgacgaggtc gtcgccatcg tcgacctgtc gcagagcgag gccgaggcgc gggtcgccgc
     3961 gatcgcggcc agccgcagcg gtgagcgaga gggggttcgt ttcgacccgc agcgctgggc
     4021 ggaggcccgc gacgcgccgg cgatcacacc ggggttgcac gcgccggtca gtcgcttcct
     4081 cgccgcgcag ctgaagatcg ggatgccgat cgacgaggtc cgcggctggc tcggcgtgcc
     4141 cgacgagcag cggctggtcg gcgggacgcg gatcgacgac tacctgctcg cgcgtgcgca
     4201 gtaccggtcg gcccacgaca gcgcgacgta tggcgtgctc gagctgcgcc acgtcgatgg
     4261 acggctcacc gagggccagc tgcgctactt tcgctggcgg gtttcgcccg gggaggcgag
     4321 ctcgcacgag ctgtcggcgg agcagttgcg ggcgctcggc gtggcgagcc acgtgctcgc
     4381 gccctgagtc ag
//
LOCUS       DASWNJ010000860         2118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_299051, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000860 DASWNJ010000000
VERSION     DASWNJ010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299051"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..838
                     /locus_tag="VGB85_14645"
     CDS             83..838
                     /locus_tag="VGB85_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0135319.1"
                     /translation="MQDLSSRSVTIRAENLSKIYGDFAALHSVSFSVRQGTVAAFLGP
                     NGAGKSTTMKILTGYLAATTGRAEVLGLDPGDPEQRIALARRLGYLPENGPLYGEMTP
                     RETLEFVAQIRGLGKGTKAAVERVVTQCAIDQVMSKPVSKLSKGYRQRVGMAQALLHD
                     PEVLILDEPTSGLDPLQIREVRQLIRELGRSKTILLSTHILGEAEAVASEVIVVGEGR
                     LAFAGTLDELRAGSTVEERFYALMDRKTKKEAA"
     gene            838..1707
                     /locus_tag="VGB85_14650"
     CDS             838..1707
                     /locus_tag="VGB85_14650"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0135320.1"
                     /translation="MSAGSGSVIGALFRKELRSFLGNPTGYVFLTLFIVASASAAFLT
                     DSFFSRNLADLATLNRWMPAILMFFVPAVTMGLWADERRSGTDELLLTLPVRDGEVVL
                     GKFLGALGVYTVGLLFSLVNVAVLAYLGDPDAGLMFATYLGYWLMGALFVAVGMVGSM
                     FTGNTTVAFILGALGCAALVFAGTAPWASAIVGVVLLGALGAFKWSIVIGRGAGIGAL
                     VGAGAGLVLWLGGNRLGWGPWFTELFAALGAPSHFESFGEGVLRLGDVLFFVGGTAVA
                     LYLCTILISRRHW"
     gene            1711..>2118
                     /locus_tag="VGB85_14655"
     CDS             1711..>2118
                     /locus_tag="VGB85_14655"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEY0135321.1"
                     /translation="MHPRIHRLVRLSSLVITAISLGYVAQRTNLRADVTEEGLSQITR
                     PTQDLIASISPERPVVIHAYVSREVPREYVAVRSQLLSILREMEARGGAGLTVRLVEP
                     EPFSPEAQEAVDNYGISPQSVTSREAGRVQQLQL"
BASE COUNT          286 a          779 c          683 g          370 t
ORIGIN      
        1 atcgtgttgc atcgtgcacg cgcagtttca cgactatcta catgtttctg tacatgtttc
       61 agcacgggtt tccaggatga ccatgcagga cctatccagc cgaagcgtca cgatccgcgc
      121 cgagaacctc tccaagatct acggggactt cgccgccctg cactcggtga gcttctcggt
      181 ccggcaaggc acggtggcgg ccttcctcgg ccccaacggc gccggcaagt cgaccacgat
      241 gaagatcttg accggctacc tggcggcgac caccggtcgc gccgaggtgc tcggcctcga
      301 tccgggggac cccgagcagc gcatcgcgct cgcgcgacgg ctcggctacc tcccggagaa
      361 cgggccgctc tacggtgaga tgaccccgcg cgagaccctc gagttcgtcg cccagatccg
      421 cggcctcggc aagggcacca aggcggccgt cgagcgggtt gtcactcagt gcgcgatcga
      481 ccaggtgatg agcaagccgg tctccaagct gtcgaagggc taccgccagc gcgtcggcat
      541 ggcccaggcg ctgctgcacg accccgaggt gttgatcctc gacgagccga cctccggcct
      601 cgaccccctg cagatccgcg aggtccggca actgatccgc gaattgggcc gcagcaagac
      661 gatcctgctg tcgacccaca tcctcggcga ggccgaggcg gtcgccagcg aggtgatcgt
      721 cgtcggcgag ggccgcctcg cgttcgccgg cacgctcgat gagctgcgcg ccggctccac
      781 cgtcgaggag cgcttctacg cgctcatgga ccgcaagacc aagaaggagg cggcctgatg
      841 tccgcgggct ctggttccgt catcggcgcg ctgttccgca aggagctgcg cagcttcctc
      901 ggtaatccca ccggctacgt gttcctgacg ctgttcatcg tcgcctcggc gtcggcggcg
      961 tttttgaccg acagcttctt cagccgcaac ctcgccgacc tggcgacctt gaaccgctgg
     1021 atgcccgcga tcctgatgtt cttcgtcccc gcggtcacca tgggcctgtg ggccgacgag
     1081 cgccgctcgg gcaccgatga gctgctgctg accctgcccg tccgcgacgg cgaggtcgtg
     1141 ctcggcaagt tcctcggcgc gctcggcgtg tacaccgtcg gcctgctgtt ctcgctcgtc
     1201 aacgtcgctg tgctcgccta tctcggcgac cccgacgccg gcctgatgtt cgcgacctac
     1261 ctcggctact ggctcatggg cgccctgttc gtcgcggtcg gcatggtcgg ctcgatgttc
     1321 accggcaaca cgaccgtcgc cttcatcctc ggcgcgctcg gctgcgccgc gctggtgttc
     1381 gccggcacgg ccccgtgggc cagcgcgatc gtcggcgtcg tgctgctcgg agccctcggc
     1441 gcgttcaagt ggagcatcgt catcggccgc ggcgcgggca tcggcgcgct cgtcggcgcc
     1501 ggcgcgggcc tggtcctgtg gctcggcggc aaccgcctcg gctggggccc ctggttcacc
     1561 gagctgttcg ccgcgctcgg ggccccgtcg cacttcgaga gcttcggcga gggcgtgctg
     1621 cgcctcggcg atgtcctgtt cttcgtcggc ggcaccgccg tcgccctgta cctctgcacg
     1681 atcctcatct cccggaggca ctggtaggcc atgcacccgc gcatccatcg actcgttcgt
     1741 ctgtcctcgc tggtcatcac cgccatctct ctcggctacg tcgcccagcg gaccaacctc
     1801 cgcgcggacg tcaccgagga gggcctgagc cagatcaccc gcccgacgca ggacctgatc
     1861 gccagcatca gccccgagcg cccggtcgtc atccacgcct acgtctcgcg cgaggtcccg
     1921 cgcgagtacg tggccgtgcg ctcgcagttg ctgtcgatcc tgcgcgagat ggaggcccgc
     1981 ggcggcgccg gcttgaccgt ccgcctcgtc gagcccgagc cgttctcgcc ggaggcccag
     2041 gaggccgtcg acaactacgg catcagcccg cagtcggtca ccagccgcga ggcgggtcgc
     2101 gtgcaacagc tccagctg
//
LOCUS       DASWNJ010000861         2250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_263831, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000861 DASWNJ010000000
VERSION     DASWNJ010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263831"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VGB85_14660"
     CDS             complement(<1..434)
                     /locus_tag="VGB85_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135322.1"
                     /translation="MAVTFARRAMIVACSLTMLAPASVRAAPASPLQQADDLNRGAID
                     LRSQAEALAGGDEGSRTQAAALLERAAQKWRDAARLLPDSAPEVRRDLFCDAAAAWQQ
                     AHELDPARIHVLRDGLETSEEFSRDPPMERNCSPASYVKYFG"
     gene            523..2154
                     /locus_tag="VGB85_14665"
     CDS             523..2154
                     /locus_tag="VGB85_14665"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0135323.1"
                     /translation="MSDPGLIGAIIEARYEILAVLGEGGMGTVYRARRLRVPGHAALK
                     VLQPELVRRPDAHERFRREASAAAAIDHPNVVQVYDSGFLPDGSAYYAMELLSGENLS
                     TTIEREHRLPWSRLRHIALQICGALRKAHSLGIIHRDLKPENCFRVVGGEDPDFIKVL
                     DFGIAKIVAPEFSDDVRLTGTGDLLGTTPYMAPEQVRGQRVDHRIDIYALGVILFQAL
                     TGQLPFDGANRAQVLAAILLNDTPTMHGVDPYARVPPELEKLVACTLHKDPTRRFQSM
                     DALAEAIRALPPDLPLAPMPEGPPRPVPGVIATRGWTEIVGGDPLAERGHTEVVDITR
                     SDGPVLGSPGPANKVASATPAPAASRSGREDILLTEDVVHPRRRLRGPAAAVLGALIV
                     LTAGMWAMLPDPPPPQAAITVPQPQVIVAPPVLPDPDTVPPPAIEEPTDEDPPSSTPV
                     VLTARDVELRVERWRKSVEAQPPKPCQFGLTREFRLEAEVRDATVTLRSGASLPADHE
                     RCREDLERSLRRRFPTSRAIKGGLARVEKIRLLLR"
BASE COUNT          320 a          868 c          740 g          322 t
ORIGIN      
        1 tccccgaaat acttcacata gctcgccgga gagcagttgc gctccatcgg cgggtcccgc
       61 gaaaactcct cgctcgtctc gaggccgtca cgcagcacgt ggatccgcgc cgggtcgagc
      121 tcgtgcgcct gctgccacgc agcggccgcg tcacagaaca gatcacgccg gacctccggt
      181 gcgctgtcag gcagcaggcg cgccgcatcg cgccacttct gggccgctcg ctcgagcagg
      241 gcggcggcct gcgtccgcga gccctcatcc ccacctgcga gcgcctcggc ctggctgcgc
      301 aggtcgatcg ccccgcggtt gagatcgtcg gcctgttgca gcggcgacgc gggtgcagcg
      361 cggacactcg ccggcgcgag catcgtcagg ctgcacgcga cgatcatcgc gcggcgggcg
      421 aatgtgacag ccatgcagcc acgatcgtag cacggaccgc gcgggcgctc tcgcccgcgc
      481 aaaggcggcg gcgaactcgg ttcatggggt tatctttgag gggtgtccga ccctggactc
      541 atcggagcga tcatcgaggc gcgctatgag atcctcgccg tgctcggcga gggtggcatg
      601 ggcacggtgt atcgcgcgcg gcgcctgcgg gtgcccggcc acgcggcgct caaggtgctg
      661 cagcccgagc tcgtccgccg gcccgacgcc cacgagcgct ttcgtcgcga ggccagcgcc
      721 gccgcggcga tcgatcaccc caacgtcgtg caggtctatg acagcggatt tttgcccgac
      781 ggctccgcct actacgcgat ggagctgctc tcgggcgaga acctgagcac gacgatcgag
      841 cgggagcatc gcctgccgtg gtcgcgcctg cgtcacatcg ccttgcagat ctgcggcgcg
      901 ctgcgcaagg cccacagcct cggcatcatc catcgcgacc tcaagcccga gaactgcttc
      961 cgcgtcgtcg gcggtgagga ccccgacttc atcaaggtgc tggacttcgg catcgccaaa
     1021 atagtcgcgc ccgagttcag cgacgacgtg cgcttgaccg gcaccggcga cctgctcggc
     1081 acgaccccgt acatggcccc cgagcaggtc cgcggccagc gcgtcgatca ccggatcgac
     1141 atctacgcgc tcggcgtgat cctgttccag gcgttgaccg gccagctgcc cttcgacggc
     1201 gccaaccggg cccaggtgct cgcggcgatc ctgctcaacg acacgccgac gatgcacggg
     1261 gtcgacccct acgcccgcgt cccgccggag ctcgagaagc tcgtcgcatg cacgctgcac
     1321 aaggacccga ccaggcgctt ccagtcgatg gacgcgctcg ccgaggcgat ccgggccctc
     1381 ccccccgact tgccgctcgc gccgatgccc gagggtccgc cgcgacccgt gcccggcgtg
     1441 atcgccacgc gcggatggac cgagatcgtc ggcggagatc cgctcgcgga gcgcggccac
     1501 accgaggtcg tcgacatcac gcgctccgat ggccccgtgc tcggcagccc cggtccagca
     1561 aacaaggtcg cgtccgccac gcccgctccg gccgcgtcgc gcagcggccg cgaggacatc
     1621 ctgctgaccg aggatgtcgt gcacccgcgc cgacgcctgc gtggccccgc cgccgcggtg
     1681 ctcggcgcgc tcatcgtctt gaccgctggc atgtgggcga tgctgcccga cccaccgcca
     1741 ccgcaggccg cgatcaccgt gccccagccc caggtgatcg tcgcgccgcc cgtgctgccg
     1801 gaccccgaca ctgtgccgcc cccggcgatc gaggagccca ccgacgagga ccccccctcg
     1861 tcgacgcccg tcgtgctgac cgcccgcgac gtcgagctgc gcgtcgagcg gtggcgcaag
     1921 agcgtcgagg cccagccccc caagccgtgt cagttcgggc tgacgcgaga gttccgcctc
     1981 gaggccgagg tccgcgacgc caccgtgacc ctgcgctccg gcgcctcgct gccggccgac
     2041 cacgagcgtt gccgcgagga cctcgagcgt tcgctgcgcc gtcgcttccc gacctcgcgc
     2101 gcgatcaagg gcggcctcgc cagggtcgag aagatccgcc tgctgctgcg ctgacggccg
     2161 tgcgatgtgc tccgcccgaa aagcgcgtca cccttgctcg tacgcccggt gctccacctg
     2221 cgtcgcctcg ccgatgtcac gatcgctcca
//
LOCUS       DASWNJ010000862         3194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_85393, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000862 DASWNJ010000000
VERSION     DASWNJ010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85393"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VGB85_14670"
     CDS             <1..497
                     /locus_tag="VGB85_14670"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0135324.1"
                     /translation="RFREDLYYRLHVFPLRLPPLRDRLEDLPQLCAAMLAEQSQRTGR
                     GGMRVTEAGVARLREHSWPGNLRELANALERATIVSTGQLLGPESFEFATRPTSPRAQ
                     VSAPAGNDGPILTLEEAQAEHIQRVLQRTGGRIYGPGGAAELLAVKPSTLQSKMKKLG
                     VPRA"
     gene            519..2675
                     /locus_tag="VGB85_14675"
     CDS             519..2675
                     /locus_tag="VGB85_14675"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY0135325.1"
                     /translation="MAKTSALALLAALCACGEPTSDSAAPTPRETCGERPPATRREDI
                     VDDLHGTRVADPYRWLEDVASPEVQGWMRVQDDHARAQLAALPGGPAFRARLAELLYI
                     DAVSAPVRRGDRIFYTRRHKDREKAIYYVREGEHGEERVLLDPNTLSPDGSVSVHGVF
                     PSRDGRLLAYKLSVNNADASTLHLRDLDSGRELPDLIDNARYASPAWTPDGRGFYYVW
                     LPRDASIPPDVLPGRAEVRFHRVGDAGPDDRIVAPATGDASRFVGVQLSRDGRHLFIS
                     HQHGWNSTDLYFQDLHEETDRPVPADIPAPGFRPLVSGQPALYDVIAWRGAFYVHTNE
                     GAPRYRVFKVDPASPERAAWREVVPESEATLEGAQVVGDRLVLSYLRDAHNELSLRDL
                     DGRPVGDIALPGLGSTSGLVGEPDDPRAYFHYTSFTQPDQIFETDVRTGAATLWREVE
                     VPVDTSRFAVEQVRYASKDGTEISMFIVRRRDLPLDGRRPTLLYGYGGFSVSLTPAFS
                     SRAVAWVEQGGVYAVPNLRGGGEYGEDWHRAGMREHKQNVFDDFAAAAEYLIAHNYTR
                     PEQLAVYGGSNGGLLVGALMTQRPELARAVVCAVPLLDMLRYHRFGAGRTWIPEYGDP
                     DIAAEFAWLHAYSPYHHVPSDRPLPALLMLTADSDDRVDPMHARKFTAAVQHVSERPA
                     WLRVERNAGHGGADLVQQTIAREGDALAFLQAELGP"
     gene            complement(2681..>3194)
                     /locus_tag="VGB85_14680"
     CDS             complement(2681..>3194)
                     /locus_tag="VGB85_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135326.1"
                     /translation="QPAPEPAKQPRRGSTRGLEIGLWTSLGMTIAWGAVAVGTGVAVA
                     HERPRDGVVDPESLDSLFMGPAYRRVFTQARVLGLQGSDRDLCAASSFAAVRGAGGDP
                     NELQDACGRFGHIRNTAIAGAVLAGVSLVATAVFAGLVVRRRNVQRGRVSVLGGPIRG
                     GAQLGLTLRF"
BASE COUNT          457 a         1311 c         1017 g          409 t
ORIGIN      
        1 gccgcttccg cgaggacctc tattatcgcc tgcacgtgtt cccgctgcgc ctgccgccgt
       61 tgcgtgaccg cctcgaggac ctcccgcagc tgtgcgccgc gatgctcgcc gagcagagcc
      121 agcgcaccgg tcgcggcggc atgcgcgtca ccgaggccgg cgtcgcccgc ctacgcgagc
      181 acagctggcc cggcaacctg cgcgagctgg ccaacgcgct cgagcgggcg acgatcgtca
      241 gcacgggcca gctgctcggg cccgagtcct tcgagttcgc gacccgcccg accagcccga
      301 gagcgcaggt cagcgcgccc gctggcaatg atggcccgat cctgacgctc gaggaggccc
      361 aggccgagca catccagcgc gttctccagc gcaccggcgg tcgtatctac ggccccggcg
      421 gcgccgccga gctgctcgcc gtcaagccct cgaccttgca gagcaagatg aagaagctcg
      481 gggtcccgcg cgcctgagcg cgtgatatcc tgcgccgcat ggccaagacc tctgccctcg
      541 ccctcctcgc tgccctgtgc gcctgcggcg agccgacgag cgacagcgcc gccccgaccc
      601 cgcgcgagac ctgcggcgag cgcccgcccg cgacccggcg cgaggacatc gtcgacgacc
      661 tgcacggcac ccgcgtcgcc gacccatacc gctggctcga ggacgtcgcc tcgcccgagg
      721 ttcagggctg gatgcgcgtg caggacgacc acgcgcgcgc gcaattggcc gcgttgcccg
      781 gtggcccggc gtttcgcgcc cgcctcgcgg agctgctcta catcgacgcc gtctcggccc
      841 cggtgcgccg cggcgaccgg atcttctaca cgcgccgcca caaggaccgc gagaaggcga
      901 tctactacgt gcgcgagggt gagcacggcg aggagcgcgt gctgctcgac cccaacacgc
      961 tctcgcccga cggcagcgtg tcggtgcacg gcgtgttccc cagccgcgac ggtcgcctgc
     1021 tcgcctacaa gctctcggtc aacaacgccg acgcctcgac cctgcacctg cgtgacctcg
     1081 acagcggccg cgagctgccc gacctgatcg acaacgcccg ctacgcgagc ccggcgtgga
     1141 cccccgacgg ccgcggcttc tactacgtgt ggctgccgcg ggacgccagc atcccgcccg
     1201 atgtgctccc cggccgcgcc gaggtccgct tccaccgcgt cggcgacgcg ggccccgacg
     1261 accgtatcgt cgcgcccgcg accggcgacg cctcgcgctt cgtcggcgtg cagctgtccc
     1321 gcgacggccg ccacctgttc atcagccacc agcacggctg gaacagcacc gacctgtact
     1381 tccaggacct gcacgaagag acagatcgac ctgtccccgc ggacatcccc gccccgggct
     1441 ttcgccccct ggtctccggc cagcccgccc tctacgacgt catcgcctgg cgcggcgcct
     1501 tctacgtgca caccaacgag ggcgccccgc gctaccgcgt cttcaaggtc gaccccgcga
     1561 gccccgagcg cgccgcctgg cgcgaggtgg tcccggagag cgaggcgacg ctcgagggcg
     1621 cccaggtcgt cggcgaccgc ctggtcctga gctacctgcg cgacgcccac aacgagctgt
     1681 cgctgcgcga cctcgacggg cgccctgtcg gcgatattgc cctgcctggc ctcggcagca
     1741 ccagcggcct cgtcggcgag cccgacgacc cgcgcgccta cttccactac acgtccttca
     1801 cgcagcccga ccagatcttc gagaccgacg tgcgcaccgg cgcggcgacg ctgtggcgcg
     1861 aggtcgaggt cccggtcgac accagccgct tcgccgtcga gcaggtccgc tacgcctcca
     1921 aggatggcac cgagatctcg atgttcatcg tccgtcgccg cgacctcccc ctcgacggcc
     1981 ggcgcccgac cctgctctac ggctacggcg gcttcagcgt cagcctcacc cccgccttct
     2041 cctcccgcgc cgtcgcctgg gtcgagcagg gcggcgtgta tgccgtgccc aacctgcgcg
     2101 gcggcggcga gtacggcgag gactggcacc gcgccggcat gcgcgagcac aaacaaaacg
     2161 tcttcgacga cttcgccgcg gccgccgagt acctcatcgc ccacaactac acgcgccccg
     2221 agcaactcgc cgtctacggc ggcagcaacg gcggcctgct cgtcggcgcc ctgatgacgc
     2281 agcgccccga gctcgcgcgc gcggtcgtct gcgccgtgcc gctattggac atgctgcgat
     2341 accaccgctt cggcgccggc cgcacgtgga tccccgaata cggcgacccc gacatcgccg
     2401 ccgagttcgc gtggctgcac gcctactcgc cctaccatca tgtcccttcg gacaggcccc
     2461 tgccggccct gctgatgctg accgccgaca gcgacgaccg cgtcgacccg atgcacgccc
     2521 gcaagttcac cgccgcggtg cagcacgtca gcgagcgccc ggcctggctc cgcgtcgagc
     2581 gcaacgccgg ccacggcggc gccgacctcg tgcagcagac gatcgcccgc gagggcgacg
     2641 cgctcgcctt cctgcaggcc gagctcgggc cctgagagcc tcaaaaccgc agcgtcagcc
     2701 cgagctgcgc gccgccgcgg atcggcccgc cgagcacgct gacgcggccg cgttgaacgt
     2761 tgcggcgccg gaccaccagc cccgcgaaca cggccgtcgc caccagcgac acccccgcga
     2821 gcaccgcccc cgcgatcgcc gtgttgcgga tgtgcccgaa gcgcccgcac gcgtcctgca
     2881 gctcgttcgg atcgccaccg gcaccacgca ccgcggcgaa gctgctcgcg gcgcacagat
     2941 cccgatcgga cccctgcaag ccgagcactc gcgcctgcgt gaacacgcga cgatacgcgg
     3001 gccccatgaa caaagagtcc aaagactcgg ggtcgaccac gccgtcgcgc ggccgctcgt
     3061 gggccaccgc gacgcccgtg ccgaccgcca cggcccccca cgcgatcgtc atgccgagcg
     3121 aggtccacag cccgatctcg aggccccgcg tcgacccgcg ccgcggctgc ttggccggtt
     3181 cgggcgcggg ttgc
//
LOCUS       DASWNJ010000863         5147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_193053, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000863 DASWNJ010000000
VERSION     DASWNJ010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193053"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VGB85_14685"
     CDS             <1..922
                     /locus_tag="VGB85_14685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03032 family protein"
                     /protein_id="HEY0135327.1"
                     /translation="VQTDGRLSLFERTFSRCMGLCADAEALWLTTLFQVWRLENALPR
                     GQEQAGYDRLYVPRVAYTTGDLDAHDLAVTRTGQVVFVNTLFSCLATLSERASFVPLW
                     RPPFVSRLAAEDRCHLNGLALADGAPAYVTLVGRSDVADGWREHRRHGGCVYDVRTNE
                     PVCEGLSMPHSPRVHAGRIWLLEAGSGHLGFVEPGRGHFEKVAFLPGYARGLCIVGDH
                     AVVGLSKLRRSRTFADLELGEQLSRRGAEPRCGLMIIDLRSGDAVHWLRLDGIVEELY
                     DVAALPGVRQPMALGLQTDEIQRTITIGEP"
     gene            952..1764
                     /locus_tag="VGB85_14690"
     CDS             952..1764
                     /locus_tag="VGB85_14690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0135328.1"
                     /translation="MHEVSRSRHALALAALLSCSPAPVSPPPDTPAAPVQPLPSPVPS
                     DMSPGPAPVAAPAAADPRAGETASVHPGINAPYRARGAARTWSRRFERDGREVHDHQA
                     EIVATLGLRPGMAVADVGAGTGLFTLAFASSVGPTGTVYAVDLIPEFLAHIGAKLKKA
                     GIGHVRLVQASDRSSELPPASVDLVFMSDAYHHLEYPQHVLASLKAALRPGGSLWVID
                     FQRDASSDPGLRRHVRAGKAEVLQELAQAGFELVREVPMLRENYILQLRPRP"
     gene            complement(1775..2542)
                     /locus_tag="VGB85_14695"
     CDS             complement(1775..2542)
                     /locus_tag="VGB85_14695"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY0135329.1"
                     /translation="MKLHGRRALVTGATSGIGRAIALRFAQEGADVAITGRRQERLEE
                     TAAAARAHGVTVVEIAAEHTRPADNARAVEAAVAGLGGLDILVNAAGVIGNDSIHAPR
                     PDEWRRILDVNLEAVYDLTHRATPHIVAGTRGSIINLSSVAGLRPYANLLAYCVSKSA
                     INTLTQCLALELAPQGVRVNAINPGVVVSELHVTGNAVTDYAAFLERARSTHPLGRPG
                     EPAEIAALATFLVSDEAGWITGGLHVVDGGRTLTSLR"
     gene            complement(2576..4180)
                     /locus_tag="VGB85_14700"
     CDS             complement(2576..4180)
                     /locus_tag="VGB85_14700"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0135330.1"
                     /translation="MAAPDSPRDAPRRPTDSDETRIHFPQSRSARLRFEAPEDLLGHT
                     LNDRYKILEQLGSGGMGTVYLAEHVLINKRVAVKVLSPAHSRKPDEVERFLREARAAS
                     RIRQENVVDITDFGYTSGGLAFLVMEYLEGEDLSTTSSREGALPWRRAVHITRQICAA
                     LTAAHAQGVIHRDMKPENVFRVSRGDDPDFIKVLDFGIAKIIDEQYDPEHPSHTNSGL
                     MGTPEYVAPELIRGLVPDVRVDIYAVGVILYRLLTGVVPFAGETFMATLTQHLMSEAV
                     PPRQAAPDAEIPADLEAVCLKSLARDRDERYSTIHEMAQALLATQAAAVLARPVAEPP
                     TPQRRLLPVLVGVVGVIVVAAIAVWAYAAGRESAPEPQAAVTAVAGVAVSIPDLPDPP
                     GRKEQAPVPAPVVEPAPVVAVVPEAPEPVEPEPVADEPPKAEPSRKSGGARPPALPER
                     LTTIELSGGMSKLAGRVRTECSKLAMRKMAVGLRVEVAASGKVKSATPKGLQSEALGT
                     CVAKIAKTAKYRAARQGSEFEYTFRM"
     gene            complement(4322..>5147)
                     /locus_tag="VGB85_14705"
     CDS             complement(4322..>5147)
                     /locus_tag="VGB85_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135331.1"
                     /translation="AIPAEVQEHYDRGARERDAGNFAVAASEFAAAYAGMPASQKELR
                     VSVLFDLVDAQRQAYGAGGRIHEREHPAAHLCAANETLTEFIDAAEKARKGKGKKSGD
                     VVRAIELRSEVRSQLDAARQSAPNLDCATAEPPTAAAVEAPAEPPPATKPDRPPRKID
                     KPLVITGAALTGFGLIMVGLMAGGLVRGNRADAEGEALVDASPTTPRDDEDLLAIDHR
                     GKVGNRMAIAGGVLGALALGSGVALLVLGLRGRPSRVAVAPAMGPRGGGLTLRWQF"
BASE COUNT          651 a         1898 c         1842 g          756 t
ORIGIN      
        1 cgtgcaaacg gacggccgcc tgtcgctgtt cgagcgcacg ttcagccgct gcatgggcct
       61 gtgcgccgac gccgaggcgc tgtggctgac gaccctgttc caggtctggc gcctcgagaa
      121 cgcgctgcca cgcggccagg agcaggccgg ttatgaccgc ctctacgtcc cgcgcgtcgc
      181 ctataccacc ggcgacctcg acgcccacga cctcgccgtc acgcgcacag gccaggtcgt
      241 gttcgtcaac acgctgttta gctgcctcgc cacgctgtcg gagcgcgcca gcttcgtgcc
      301 gctgtggcga ccgcccttcg tgtcgcgcct ggccgccgag gaccgctgtc acctcaacgg
      361 cctcgcgctc gcagatggtg ccccggccta cgtgacgctc gtcggtcgca gcgacgtcgc
      421 cgacggctgg cgcgagcacc ggcgtcatgg cggttgtgtt tacgacgtcc gcaccaacga
      481 gccggtgtgc gagggcctgt cgatgcccca ctcgccgcgc gtgcacgccg gacggatctg
      541 gctgctcgag gccggcagcg ggcacctggg cttcgtcgag cccggccgcg ggcattttga
      601 aaaggtcgcg ttcttgcccg gctacgcgcg cgggctgtgc atcgtcggcg accacgcggt
      661 cgtcggcctg tcgaagctgc gccgcagccg gaccttcgcc gacctcgagc tcggcgagca
      721 gttgagtcga cgcggcgccg agccgcgctg cggcctgatg atcatcgacc tgcgcagcgg
      781 cgacgcggtg cactggctgc gcctcgatgg aattgtcgag gagctgtacg acgtcgccgc
      841 cctgcccggc gtccgccagc cgatggcgct cggcctgcaa accgacgaga tccagcgaac
      901 gatcacgatc ggcgagccct gagcggcgat ccggtgcaag actgacgggc gatgcacgag
      961 gtctcacgat ccagacatgc gctcgccctc gcggcgctgc tgtcgtgctc gcccgccccg
     1021 gtgtcgccgc cgccggacac gcccgcggcg ccggtgcagc cgctcccatc accagtccct
     1081 tcggacatgt ccccggggcc agcccccgtc gcggcacccg cagcagcgga cccgcgcgcg
     1141 ggcgagaccg cgagcgtcca tcccggcatc aacgccccgt accgggcccg cggggcggcg
     1201 cgcacgtggt cgcgccgctt cgagcgagac ggccgcgagg tccacgatca tcaggccgag
     1261 atcgtcgcca cgctggggct gcgccccggc atggcggtag ccgacgtcgg ggccggcacg
     1321 gggctgttca ccctcgcctt cgccagctcc gtcggcccca cgggcaccgt gtacgccgtc
     1381 gacctgatcc ccgagttcct cgcgcacatc ggcgcgaagc tgaaaaaggc cggcatcggc
     1441 cacgtgcggc tcgtgcaggc cagcgaccgc agcagcgagc tgccgccggc cagcgtcgac
     1501 ctcgtgttca tgagcgacgc ctaccaccac ctcgagtacc cgcagcacgt gctcgcgagc
     1561 ctcaaggccg cccttcgtcc gggcgggagc ttgtgggtga tcgacttcca gcgcgacgcg
     1621 agcagcgacc ccggcctgcg caggcacgtg cgcgccggca aggccgaggt gctccaggag
     1681 ctcgcgcagg ccggcttcga gctggtccgc gaggtgccga tgttgcggga gaactacatc
     1741 ctgcagctgc gcccgcgccc gtgaggccgg ccttttagcg cagcgaggtc agcgtgcgcc
     1801 cgccgtcgac gacgtgcagc ccgccggtga tccatccggc ctcgtcgctg acgagaaagg
     1861 tcgccagcgc ggcgatctcc gccggctcac cggggcggcc gagcgggtgc gtgctgcggg
     1921 cgcgctcgag gaacgccgcg tagtcggtga ccgcgttgcc ggtgacgtgc agctcgctga
     1981 cgaccacacc cgggttgatc gcgttgacgc gcacgccctg cggcgccagc tcgagcgcga
     2041 ggcactgcgt cagggtgttg atcgccgact tgctgacgca gtacgccagc aggttggcgt
     2101 agggtcgcag cccggcgacg ctcgagaggt tgatgatcga cccgcgcgtg ccggcgacga
     2161 tgtgcggggt ggcgcgatgc gtgaggtcgt agacggcctc gaggttgacg tcgaggatcc
     2221 gccgccactc gtccggcctc ggcgcgtgga tcgagtcgtt gccgatcacg cccgcggcgt
     2281 tgacgaggat gtcgaggccg ccgagaccag cgaccgccgc ctcgaccgcg cgcgcgttgt
     2341 cggccggtcg cgtgtgctcc gctgcgatct cgacgaccgt cacgccgtgg gcccgcgccg
     2401 ccgcggcggt ctcttcgagc cgctcctgcc tgcgacccgt gatcgccacg tcggcgccct
     2461 cctgggcgaa tctgagggcg atcgcgcggc cgatgccgct ggtcgctccg gtcacgaggg
     2521 ctcgccgtcc gtgcagcttc atcgccgccg accctagcac cgcgacccgg actcgtcaca
     2581 tgcgaaacgt gtactcgaac tcggagccct ggcgcgccgc ccggtacttc gcggtctttg
     2641 cgatcttcgc cacgcaggtg ccgagcgcct ccgactgcag gcccttgggc gtcgccgact
     2701 tgaccttgcc gctcgccgcg acctcgacgc gcaggccgac ggccatcttg cgcatcgcca
     2761 gcttggagca ctccgtacgc acgcgcccgg ccagcttgct catgccgccc gagagctcga
     2821 tcgtcgtcag gcgctcgggc aacgccggcg ggcgcgcgcc gcccgacttg cggctcggct
     2881 cggccttcgg cggctcgtcc gcgaccggct cgggctccac cggctcgggc gcctcgggga
     2941 ccacggcgac gaccggcgcc ggctcgacca cgggcgccgg cacgggcgcc tgttctttcc
     3001 tacctggcgg atcgggcagg tccgggatgc tgaccgcgac ccctgcgacg gcggtcaccg
     3061 cggcctgcgg ctcgggtgcc gactcacggc cggcggcgta ggcccacacg gcgatcgcgg
     3121 cgacgacgat gaccccgacg accccgacga gcaccggcaa gagccggcgc tgcggcgtcg
     3181 gcggctccgc gaccgggcgc gcgagcacgg cggccgcctg ggtggcgagc agcgcctggg
     3241 ccatctcgtg gatcgtcgag tagcgctcgt cgcggtccct ggcgaggctc ttgaggcaca
     3301 cggcctcgag gtccgcgggg atctcggcgt cgggcgcggc ctgtcgcggc ggcacggcct
     3361 ccgacatcag gtgctgtgtc agcgtcgcca tgaaggtctc gcccgcgaac ggcacgaccc
     3421 cggtcagcag gcgatacagg atcacgccga ccgcgtagat atcgacgcgg acatctggca
     3481 ccagcccgcg gatcagctcc ggcgcgacgt actccggcgt gcccatcagg ccgctgttgg
     3541 tgtgcgacgg gtgctccggg tcgtactgct cgtcgatgat cttggcgatg ccgaagtcga
     3601 ggaccttgat aaagtcgggg tcgtcgccgc gtgacacgcg gaacacgttc tccggcttca
     3661 tgtcgcggtg gatgaccccc tgcgcgtgcg cggccgtcag cgcggcgcag atctggcggg
     3721 tgatgtgcac cgcgcggcgc cacggcagcg cgccctcgcg gctcgaggtg gtcgagaggt
     3781 cctcgccctc gaggtactcc atcaccagga acgccaggcc gccgctcgtg tagccgaagt
     3841 cggtgatgtc gacgacgttc tcctggcgga tccgggacgc cgcgcgggcc tctcgcagga
     3901 agcgctcgac ctcgtcgggc ttgcggctgt gcgccgggct cagcaccttg accgcgacgc
     3961 gcttgttgat cagcacgtgc tcggccagat acacggtgcc catgccgccc gagccgagct
     4021 gctcgagaat tttgtaacgg tcgttgagcg tgtgcccgag caggtcctcg ggcgcctcga
     4081 agcgcagcct cgccgaccgc gactgcggga agtggatccg cgtctcgtcg ctgtcggtgg
     4141 ggcgcctggg cgcgtcgcgc gggctgtccg gagcggccat ccgacccgca gcatagcgga
     4201 ttatccgccc gtcatgcgcc cgcccgcggg gcccggagcg caggtccgcg gcggcgcgat
     4261 ctgacccaag ggccagcttg tgacgcggcc gcgaggacgg cctgtggacc cgccgcccgg
     4321 cctagaactg ccagcgcagc gtcagaccgc cgccgcgcgg gcccatggcc ggggcgaccg
     4381 ccaccctgga cggtcgtccg cgcagcccga gcaccagcag cgcgacgccg ctgccgagtg
     4441 cgagcgctcc gagcacgccg ccggcgatcg ccatgcgatt gccgaccttg ccgcggtggt
     4501 cgatcgccag caggtcctcg tcgtcgcgcg gggtcgtcgg gctcgcgtcg acgagcgcct
     4561 cgccctcggc atcggcacgg ttgccgcgca cgaggccgcc ggccatcagc ccgaccatga
     4621 tcagcccgaa gccggtcagc gcggccccgg tgatcaccag cggcttgtct attttgcgcg
     4681 gcggacggtc cggcttggtc gcaggcggcg gctccgcggg ggcctcgacc gccgcggccg
     4741 tcggcggctc cgcggtcgcg caatcgaggt tcggggcgct ctggcgggcg gcgtcgagtt
     4801 ggctgcgaac ctcgctgcgc agctcgatcg cgcggacgac gtcgcccgac ttcttgccct
     4861 tgcccttgcg cgccttctcg gcggcgtcga tgaactcggt cagcgtctcg ttggcggcgc
     4921 acaggtgggc cgccgggtgc tcgcgctcgt ggatccggcc gccggcgccg taggcctggc
     4981 gctgggcgtc gacgaggtcg aacagcacgc tgacgcgcag ctccttctgc gaggccggca
     5041 tcccggcgta ggcggcggcg aactccgagg ccgcgaccgc gaagttaccg gcgtcacgct
     5101 cgcgggcgcc gcggtcgtag tgctcctgga cctcggcggg gatggcg
//
LOCUS       DASWNJ010000864         3006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_299096, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000864 DASWNJ010000000
VERSION     DASWNJ010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299096"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..>3006
                     /locus_tag="VGB85_14710"
     CDS             237..>3006
                     /locus_tag="VGB85_14710"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0135332.1"
                     /translation="MSHDGTLPGTGGIDEVGDTHDRSGARPATDGGEAPPGAVEWTPP
                     AEFDEYRIVRLLGQGSMGRVYLARDTVLDRPVAVKFITHVTPDAEDRERFLVEARAVA
                     RVQHPNVVTIHRVGELEGHPYLITEYVAGKSLSDLAVPVGWRKVLELAVGIARGLAAA
                     HRQGVLHRDIKLANAMLSATGEVKLLDFSLAKLLDPVFDPSHEGGPLRLPPTDRANDR
                     TLSPREARSSSSSSLMTNSRISSVWQHGASGASSGHWPITESAPSLTMHGTLLGTPNY
                     MAPELWRNEEATRRSDVYALGVLMYILASGRPPTEAEALVELAMRVQEDEPPSLLDRA
                     PRCDPRLAGIIDRCLRRDPFERFTSADELLAALEAVAPTTRELAIPEGNPYRGLEAFE
                     SRHRALFFGRGGEIRAVLERLRAGALVVVAGDSGVGKSSLCKAGVVPAVEEGALDPQR
                     TWTSATMTPGRYPLQTLTATLARLFDMGEEQVSATLTGAGEGAIRTLRRQLGDGRGAL
                     LMVDQLEELATLGAPHEVAVVGPLLARLASGMPGLRVLATVRGDFLTRVAQLPGIGDE
                     LAQAIYFLRPLAPEGAREAIVGPARVKGVGFASEVLVDQLVMAGSQGSLPLLQFALAE
                     LWEIRDQATRSITAEDLRKIGGVSGALARHADAALAELIPEQRVAARRVLMRLVTIDD
                     TRAVRTEDELTAGSAAAQAALKALVRSRLVLVREAGDQAVYEIAHEALIGGWAALGAW
                     LDEEREARAIHHRLELAVAEWERLGRGRDGLVAGAPFRELRTLPPAILRPREREYIAA
                     CHAAIARTRMLRRLALAAVPVLVVLAAGAVWLKGQVDLRETVDAHVATARDQLAEVAE
                     LTARAEVQRAEAFAAHDAGDDKRGEALWVRYAAEVPEIERGLARAAQQLETALSLDAS
                     RDDVRGRLL"
BASE COUNT          380 a         1094 c         1167 g          365 t
ORIGIN      
        1 ccacccggcg caggcggaga gcaggagcag ggcggtggag gcgaggcggc gtgcggacat
       61 cgggcgagac cttgaggaag gaggatgcca cccgaacgcc ggtcacggtg cgccgcatac
      121 agccgcggct gcgatcgccg acggctgaat ttgcccgatg tgcctggtga cggccgtgct
      181 tgcgggcgcg cctgcgccag tgccaagctg ggcgcgatga tcgagggctg ttcttcgtga
      241 gccacgacgg cacactgccc gggacgggcg gcatcgacga ggtcggcgac acccacgacc
      301 gcagcggcgc ccggccggcg acggacggcg gcgaggcgcc gcctggcgcg gtcgagtgga
      361 cgccgccggc ggagttcgac gagtaccgga tcgtgcgcct gctcgggcag ggcagcatgg
      421 gccgggtcta tctcgcgcgc gacacggtgc tcgaccggcc ggtcgcggtg aagttcatca
      481 cccacgtgac gcccgacgcg gaggaccgcg agcgcttcct cgtcgaggcc cgcgcggtcg
      541 cccgcgtgca gcaccccaac gtcgtcacga tccaccgcgt cggcgagctc gagggccatc
      601 cgtacctgat caccgagtac gtcgccggca agagcctcag cgatctggcg gtgccggtcg
      661 ggtggcgcaa ggtgctggag ctcgccgtcg gcatcgcccg cgggctggcg gcggcgcacc
      721 gccagggcgt gctgcaccgc gacatcaagc tcgccaacgc gatgctgagc gcgaccggcg
      781 aggtcaagct gctcgacttc agcctcgcca agctgctcga cccggtgttc gacccgtcgc
      841 acgagggcgg tccgctgcgc ctgccgccga ccgaccgcgc caacgaccgc acgctgtcgc
      901 cccgcgaggc gcgtagcagc agcagcagca gcctgatgac gaactcgcgg atcagctcgg
      961 tgtggcagca cggcgcgtcg ggggcgtcgt cggggcactg gccgatcacc gagagcgccc
     1021 cctcgttgac gatgcacggg accctgctcg gcacgccgaa ctacatggcg ccggagctgt
     1081 ggcgcaacga ggaggcgacc cgccggtccg acgtgtacgc gctcggggtg ctgatgtaca
     1141 tcctcgcgtc cggccgcccg ccgaccgagg ccgaggcgct cgtcgagctg gcgatgcgcg
     1201 tgcaggagga cgagccgccg tcgctgctcg accgggcccc gcgctgcgac ccgcggctcg
     1261 ccggcatcat cgaccgctgc ctgcggcgcg accccttcga gcgcttcacc tcggccgacg
     1321 agctgctcgc ggcgctcgag gcggtggccc cgacgacccg cgagctggcg atccccgagg
     1381 gcaacccgta ccgcgggctc gaggcgttcg agtcgcgcca ccgggcgctg ttcttcggcc
     1441 gcggcggcga gatccgggcg gtgctcgagc ggctgcgggc cggcgcgctc gtcgtcgtcg
     1501 ccggcgactc gggcgtcggc aagtcctcgc tgtgcaaggc tggcgtcgtg ccggcggtcg
     1561 aggagggcgc cctcgatccg cagcgcacgt ggaccagcgc gacgatgacc ccggggcgct
     1621 acccgctgca gacgctgacc gccacgctcg cgcgcctgtt cgacatgggc gaggagcagg
     1681 tcagcgcgac gctgacgggc gccggcgagg gggcgatccg cacgctgcgg cgccagctcg
     1741 gcgacgggcg cggggccctg ctgatggtcg accagctcga ggagctggcg acgctcggcg
     1801 ccccgcacga ggtcgcggtg gtcgggccgc tgctcgcccg cctcgcctcg ggcatgccgg
     1861 gcctgcgcgt gctggcgacc gtgcgcggcg acttcctgac gcgcgtcgcc cagctgccgg
     1921 gcatcggcga cgagctggcg caggcgatct atttcctgcg gccgctggcg cccgagggcg
     1981 cgcgcgaggc gatcgtcggg ccggcgcggg tcaagggcgt cggcttcgcc agcgaggtgc
     2041 tcgtcgacca gctggtcatg gccggctcgc agggcagctt gccgctgctg cagttcgcgc
     2101 tcgccgagct gtgggagatc cgcgaccagg cgacgcgcag catcaccgcc gaggacctgc
     2161 gaaagatcgg cggcgtcagc ggggcgctcg ccaggcacgc cgacgcggcg ctcgccgagc
     2221 tgatccccga gcagcgggtc gcggcccggc gcgtgctgat gcgcctcgtc acgatcgacg
     2281 acacccgcgc cgtgcggacc gaggacgagc tgaccgccgg ctccgcggcg gcgcaggcgg
     2341 ccctcaaggc gctggtgcgc tcgcgcctgg tgctggtccg cgaggccggc gaccaggcgg
     2401 tctacgagat cgcccacgag gcgttgatcg gcgggtgggc ggcgctcggc gcctggctcg
     2461 acgaggagcg cgaggcccgg gcgatccatc atcgcctcga gctcgcggtc gccgagtggg
     2521 agcgcctcgg acgcggccgc gacgggctgg tcgccggggc gccgttccgg gagctgcgca
     2581 cgctgccgcc ggcgatcctg cgcccgcgcg agcgcgagta catcgccgcc tgccacgccg
     2641 cgatcgcgcg cacgcggatg ctgcggcgcc tcgcgctcgc ggcggtgccg gtgctcgtcg
     2701 tgctcgccgc gggggccgtc tggctcaagg gacaggtcga cctgcgcgag accgtcgacg
     2761 cccacgtcgc caccgcgcgc gaccagctcg ccgaggtcgc cgagctgacg gcccgcgccg
     2821 aggtgcaacg cgccgaggcg ttcgcggccc acgacgccgg ggacgacaag cgcggggagg
     2881 cgctgtgggt ccgctatgcg gcggaggtcc cggagatcga gcgcgggctg gcgcgcgcgg
     2941 cccagcagct cgagacggcg ctcagcctcg acgccagccg tgacgacgtc cgcgggcgcc
     3001 tgctcg
//
LOCUS       DASWNJ010000865         3719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_327410, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000865 DASWNJ010000000
VERSION     DASWNJ010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327410"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VGB85_14715"
     CDS             <1..617
                     /locus_tag="VGB85_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135333.1"
                     /translation="SSASAADVKRRGGQVEGTFGASLCIPGKGDCKSADDVSGKTGPS
                     FGMGFTLGVRPIKYLMIGGAYNLGFFNPDYKDGPVDVYKSAYQNSVFGVIRAIIPVWR
                     VDIGLEVAPGWSRQTFKVKEGITSTFLGDKVYSQGFALKTAPVVDIFITRNFFVGAKV
                     DFIFNFHKQVCTSRGSSSGCETLEDNNQASVHQMLVGLHVGGTF"
     gene            complement(824..2929)
                     /locus_tag="VGB85_14720"
     CDS             complement(824..2929)
                     /locus_tag="VGB85_14720"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135334.1"
                     /translation="MMVRAGLLSDDQLAAVQARSAERKLSFVESLLELGVIDEDSVVS
                     FLHSKLMIPRVGASVLDTVDKGALLRIPAELAQKLAVLPISADETGNLTIAMVDPTDM
                     RAVDAISAHTGAYLVRAVAPLRIIRAAISKHYNIPMPEAAPALSAPAVAPRPAAVTLP
                     ASAAGPAPARVPPSVTPPATTSPTANPPAPTAPAEPDASLAAGQRTITGTGSASASGS
                     LHVAPAAVPPQSGGVAQPSVVIPRPATAPAVAPDAHTLPGRNKASASGAHPAASGPTE
                     LPRAPEPAPVAPTPTSTPDAPPTSATSTTSSTSSIKPSAVLRPSAVLQPSAVLMPGGT
                     PVSTPRPAVVLPASAMSSAPYGAPIMQAPVPAVFIPNPTQPPGPKPGAEAKAPLPSQE
                     NTVIGHAPVGGQPNASPPAPVQPAPSQASAAQATARRASAPQAAAPLPADFQPGGTAT
                     FEPAPARKPATTPQPAVADAPEKRPVEVTGPSETMTGGSRSDVVDIPLDDLDDDAPPT
                     PTSTPTINPEDRTTATWTPPQFGVANEVIPLSPEAFHRLLPRFATVKTRDEVTDLLLD
                     FLAEGFARVIMFTHIKGEIRGRDARGEDLMVEAVRQIRIPATGPSLFSGVIERRQPYF
                     GAMRTDTAIDAAFYSALGGVDGVVLCLPVILRDKVPLLVFASGSHNPVDPRSLHDLTH
                     EVAAALERIIVADKTRGAR"
     gene            3137..>3719
                     /locus_tag="VGB85_14725"
     CDS             3137..>3719
                     /locus_tag="VGB85_14725"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0135335.1"
                     /translation="MRYLDHNATTPLDPRVRDAVIAALSDDRLQANPSSVHGPGQRAR
                     AVLEQARRAVARAVGAAALELTFTSGGTEADNLAILGAARALRAAGRPAGVLTTALEH
                     PAVLGAARALAHEGHPSLRLPVDGQGRVDAGQLRAMLHAHPEVGLVSLAAVNHELGNV
                     YDVAGLVRVVREAAPQALVHCDGVQALGKISVDL"
BASE COUNT          481 a         1172 c         1539 g          527 t
ORIGIN      
        1 cgtcgagcgc ctccgcggcg gacgtcaagc gtcgcggcgg tcaagtcgag ggcaccttcg
       61 gcgcgtcgct gtgcatcccc ggcaagggtg actgcaagtc ggccgacgat gtgtcgggca
      121 agaccggacc gtcgttcggc atgggcttca cgctcggcgt gcgcccgatc aagtacctga
      181 tgatcggcgg cgcctacaac ctcggcttct tcaaccccga ctacaaggac ggcccggtcg
      241 acgtctacaa gtcggcctat cagaacagcg tgttcggggt catccgcgcg atcatcccgg
      301 tgtggcgcgt cgacatcggc ctcgaggtcg cgcccggctg gtcgcgccag accttcaagg
      361 tcaaggaggg catcactagc accttcctcg gcgacaaggt ctactcccag ggcttcgccc
      421 tgaagaccgc ccccgtggtc gacatcttca tcacgcggaa cttcttcgtc ggcgccaagg
      481 tcgacttcat cttcaatttt cataagcagg tctgcacgtc gcgcggcagc agcagcggct
      541 gcgagaccct cgaggacaac aaccaggcct cggtgcacca gatgctcgtc ggcttgcacg
      601 tcggcggcac gttctgatcc gcagttcgct gcttcgcggg ctctgtacgg gcgtgattga
      661 ggggcgcggc ttcccgacct tccgccacga cgcggggtcg agttccgcgg gctctgtacg
      721 gcgtgatcga ggggcgcggc ttcccggccg tccgccacga cggggggccc cgcgtctccc
      781 ggtcacaccc cggccgggat gttgggtcga gccgggagcc gggctagcgc gcgccgcgag
      841 tcttgtcggc gacgatgatg cgctcgaggg ccgcggcgac ctcgtgggtg aggtcatgca
      901 gcgagcgcgg gtcgacgggg ttgtgcgagc cggaggcgaa gaccagcagc gggaccttgt
      961 cgcgcaggat gacggggagg cacaggacga cgccgtcgac gccgccgagg gcggaataga
     1021 aggcggcgtc gatcgccgtg tccgtacgca tcgcgccgaa gtaaggctgg cggcgctcga
     1081 tgacgccgga gaacagcgag gggccggtcg cggggatccg gatctggcgc acggcctcga
     1141 ccatcaggtc ctcgccgcgg gcgtcgcggc cgcggatctc acccttgatg tgcgtgaaca
     1201 tgatgacgcg cgcgaagccc tcggccagga agtcgagcag gaggtcggtg acctcgtcgc
     1261 gggtcttgac ggtggcgaag cgcggcagga ggcggtggaa cgcctcgggg gacagcggga
     1321 tgacctcgtt ggcgacgccg aactgcggcg gcgtccaggt ggccgtggtg cggtcctcgg
     1381 ggttgatcgt gggggtgctc gtcggcgtcg gcggagcgtc gtcgtcgagg tcgtcgagcg
     1441 ggatgtcgac gacgtcggag cgcgagccgc cggtcatcgt ctcgctcggg ccggtgacct
     1501 cgaccgggcg cttctccggg gcgtcggcga cggccggctg cggcgtggtc gcgggcttgc
     1561 gggcgggggc cggctcgaag gtcgcggtgc cgccgggctg gaagtcggcc gggaggggag
     1621 cggcggcttg cggggcggag gcgcggcggg cggtcgcctg cgcggcggag gcctgagatg
     1681 gagcgggctg gactggagcg ggcggtgacg cgttcggctg cccgccgacc ggcgcgtggc
     1741 cgatgaccgt gttctcctgc gagggcagcg gggccttggc ctcggcgccc ggcttgggac
     1801 cgggcggctg cgtcgggttc gggatgaaca cggcgggcac cggcgcttgc atgatcggcg
     1861 cgccgtaggg cgccgagctc atcgcgctgg caggcagcac gacggcgggt cgcggggtcg
     1921 agaccggagt gccgccgggc atcaggaccg ccgagggttg cagcacggcg gacgggcgca
     1981 gcacggcgga gggcttgatc gagctggtcg agctggtcgt gctggtcgcg ctggtcggtg
     2041 gtgcgtccgg ggtgctcgtc ggcgtgggcg cgacgggggc cggctcggga gcgcggggca
     2101 gctcggtcgg gccgctcgcg gccgggtgcg cgcccgaggc cgaggccttg ttacggccgg
     2161 gcaaggtgtg ggcatcgggg gcgacggccg gggccgtggc gggccgcggg atcacgacgg
     2221 agggctgggc cacaccgcca gattgcggcg ggacggcggc gggcgccacg tgcagcgagc
     2281 ccgaggcgga cgcggagccg gtgccggtga tcgtccgctg gcccgcggcg agcgaggcgt
     2341 cgggctccgc gggggcggtc ggggccggcg ggttcgccgt gggagaggtg gtggcaggag
     2401 gggtgaccga cgggggcacg cgggcggggg cggggcccgc ggcggaggcc ggcagggtga
     2461 ccgcggcagg acgcggggcg acggcgggcg cgctgagggc cggcgccgcc tcgggcatcg
     2521 ggatgttgta gtgcttcgag atcgcggcgc ggatgatgcg aagtggggcg acggcgcgga
     2581 ccaggtacgc gccggtgtgc gccgagatgg cgtcgacggc tcgcatgtcc gtcgggtcga
     2641 ccatcgcgat cgtcaggttc ccggtctcgt cggcggagat cgggagcacg gcgagcttct
     2701 gcgccagctc ggcggggatc cgcagcaggg cgcccttgtc gacggtgtcg aggaccgacg
     2761 cgccgacgcg agggatcatc aacttgctgt ggaggaacga gacgacgctg tcctcgtcga
     2821 tgacgccgag ctcgagcagg gactcgacga acgagagctt gcgctccgcg ctgcgggcct
     2881 gcacggcggc gagttgatcg tccgagagca ggcccgccct gaccatcatg gttccgagga
     2941 gactcaagcg ccgatgatga ttcatcgccg gcgccgcgca gtcaacaagg tgacaccctt
     3001 cgcgcgaagg gcggccgctc gggggccggg cccaccagtt ggcgggagat ccgtgacctg
     3061 ggcgacgcgc gctggcccgg gtaggcacgt cggcgagggg agatagcccg cgcgcgccgt
     3121 gctatacggg ccgcgggtgc gctacctcga ccacaacgcg accaccccgc tcgatccgcg
     3181 ggtccgggac gctgttatcg cggccctgtc cgacgatcgg ctacaggcca acccgtcgag
     3241 cgtgcacggg ccggggcagc gggcgcgcgc ggtgctcgag caggcgcggc gggccgtggc
     3301 gcgggcggtc ggggcggcgg cgctggagct gaccttcacc tccggcggca ccgaggccga
     3361 taatttggcg attctcgggg ctgcgcgggc gttgcgggcc gcggggcggc cggcgggggt
     3421 gttgacgacg gcgctcgagc acccggccgt gctcggggcc gcgcgggcgc tcgcgcacga
     3481 gggtcatccc tcgctgcgcc tgccggtgga cgggcagggg cgcgtggacg ccgggcagct
     3541 gcgggcgatg ttgcatgcgc atccggaggt cgggctggtc tcgctggcgg cggtcaacca
     3601 tgagctcggc aacgtttatg acgtcgcggg gctcgtgcgg gtcgtgcgcg aagccgcacc
     3661 gcaggcgctg gtccactgcg acggtgtaca ggccctcggg aaaatctcgg tggatctcc
//
LOCUS       DASWNJ010000866         3426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_651837, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000866 DASWNJ010000000
VERSION     DASWNJ010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651837"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(294..533)
                     /locus_tag="VGB85_14730"
     CDS             complement(294..533)
                     /locus_tag="VGB85_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135336.1"
                     /translation="MLRLLEVVCVVLLVLSSEQTFEEIHYAHALPLIGTRQTLLHPGS
                     GVGPMGERIFTGAGRRSQAAAIAVSSRSRDRDLPC"
     gene            612..1217
                     /locus_tag="VGB85_14735"
     CDS             612..1217
                     /locus_tag="VGB85_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135337.1"
                     /translation="MFRIAPRTPTTVDFSEVLKRSGVDAEQQKRVKKAQDLLRDLPEE
                     TPADLKRQIVEATFRNFDVPTQKIIDAATLEVEALEAFIAAGGATTQKILDDGSARIA
                     ELEAEIAEIRASMATAVSDQQIRDRATQQQIAGVTPILAFFAQSSGLQQPFFAQTSGN
                     NGGGTAQPFFPPPPPEVQTNSFFDRPASSGFSDIVDDVDLE"
     gene            1295..3226
                     /locus_tag="VGB85_14740"
     CDS             1295..3226
                     /locus_tag="VGB85_14740"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEY0135338.1"
                     /translation="MKRSAPSASLLWCLALASACKGEAAGDTGGGSTGSSGSTGDTGE
                     TPTTGETPAPLQGIADLHLHMFAEEAFGGGWFHGSHRGAGEVALAPCDGGEPGDHARL
                     KDDLAPLLGSCEGITLAELAAQVPLIAVISAEGGGEVVSEFVSTIPGTDGDTGKHADR
                     TTGWPDLAGWPRWDTIAHQQVWEEQLHDAYMAGLRVEVMSAVSLDWLCRAVPPENVER
                     PQCDEMADVRLQLEQAHEFVATHDWAEIALTAADARRIVEEDRLAIVLSIEASHLMKD
                     GDWRPQLDELYALGVRTLQPVHQLDNRFGGAAPHNSIFHVAQYAESCHIDEDCGLTTS
                     TLTLGFDVDADCKNTLGLTDDGEALVAEMMDRGMLVDVAHLSERGIKDVHDLAVAREH
                     YPIYVSHGHFREIMTKERQREEKTTPAWVIEILRETGGMIGLRTGHEEVNSYDASAVA
                     NTCHGSSRSFAQAYDFGRLGLKVAIGLGSDLNGFIQQTRPRFGAAACSASFTTEAQCQ
                     AREQREAGPPPLGNDLDEAGLGHVGLLPALLADLDQLGTDTAPLRSSADDFVRMWERA
                     ASPRSGPAANADDLDVSGVTVLPVHSLREAEFPAQCGDSYCAGALEAGAECRFAAECV
                     SGECVGAGECGKPRGVCAG"
BASE COUNT          537 a         1198 c         1230 g          461 t
ORIGIN      
        1 agaagcagcc gcccgcgaag tagccgaact cccgcgcgcc gctgtcctga gcgaccgggg
       61 cgggcgcgtc cttctccttg ttggcggtct gggtcgggtc gacgctgccg caggcggcga
      121 cggcggtgag catgcacagg aaaattcgtc gggtcatgat gctggcgtcc tgacccggcg
      181 catacgtcgg ccggggggac caggttacat cgcccgtgaa ttcaccagct cgaccccgac
      241 cgcacagcag cagggtcggt ggcgcacacg gccccgggtc gacgatctcg cggtcagcac
      301 gggagatcgc ggtcgcgcga tcggctcgaa acggcgatcg cggccgcctg cgagcggcgt
      361 ccggcacccg tgaaaatccg ttcgcccatg gggcccacgc cggagccggg atggagtaaa
      421 gtctggcgcg tgccaataag ggggagagca tgggcataat gaatttcttc aaaggtctgt
      481 tcggaggaaa gaaccaacag gacgacgcaa acgacttcga ggagacgcaa cacgtgcttc
      541 caccatcacc ggtccgtgtc cccacgcctg cccctgctcc cgcgcctgcg cctgcacccc
      601 cgccgccgcc ggtgttccgc atcgctccgc gaacgccgac gaccgtcgac ttctcggagg
      661 tgctcaagcg ctcgggcgtc gacgccgagc agcagaagcg ggtgaagaag gcgcaggacc
      721 tgctgcgcga cctgccggag gagacgccgg cggatctcaa gcggcagatc gtcgaggcga
      781 cgtttcgcaa cttcgacgtg ccgacgcaga agatcatcga cgcggcgacc ctcgaggtcg
      841 aggcgctcga ggccttcatc gcggccggcg gcgcgacgac gcagaagatc ctcgacgacg
      901 gctcggcccg catcgccgag ctcgaggcgg agatcgccga gatccgggcc tcgatggcga
      961 ccgccgtgtc cgaccagcag atccgcgacc gggcgaccca gcagcagatc gccggcgtca
     1021 cgccgatcct cgccttcttc gcgcagagct ccgggctgca gcagccgttc ttcgcgcaga
     1081 ccagcggcaa caacggcggc ggcacggccc agccgttctt cccgccgccg ccgcccgagg
     1141 tccagaccaa ctcgttcttc gaccgtcccg cgagctccgg cttctcggac atcgtcgacg
     1201 acgtcgacct cgagtagcgt acctgtcctg caggacaggt gccgtcgggg cgcgcgcttg
     1261 accggtttcg cgccggtcgc taccgtcgcg tcgcatgaag cgatctgccc cgtccgcgag
     1321 tcttctgtgg tgcctcgcgc tcgccagcgc ctgcaagggt gaggcggcgg gggacaccgg
     1381 gggcggctcg actggttcgt caggttcgac tggcgacacc ggcgagacgc cgacgacggg
     1441 cgagacaccg gcgccgctgc aggggatcgc ggacttgcac ctgcacatgt tcgccgagga
     1501 ggcgttcggc ggcgggtggt tccacggcag tcaccgcggc gcgggcgagg tggcgctggc
     1561 gccgtgcgac ggcggtgagc cgggggacca cgcgcggctc aaggacgacc tggcgccatt
     1621 gctcgggagc tgcgagggca tcacgctggc ggagctcgcg gcccaggtgc cgctgatcgc
     1681 cgtgatctcg gcggagggcg gcggcgaggt cgtcagcgag ttcgtcagca cgatccccgg
     1741 gaccgacggc gacaccggga agcacgccga tcgcacgacc ggctggcccg acctcgccgg
     1801 ctggccgcgc tgggacacga tcgcccacca gcaagtctgg gaggagcagc tgcacgacgc
     1861 gtacatggcc gggctgcgcg tcgaggtgat gtcggcggtg tcgctcgact ggctgtgccg
     1921 cgcggtgccg ccggagaacg tcgagcgccc gcaatgcgac gagatggccg acgtccggct
     1981 gcagctcgag caggcccacg aattcgtggc gacccacgac tgggccgaga tcgcgctgac
     2041 cgccgcggac gcgcgccgga tcgtcgagga ggaccggctg gcgatcgtgc tgtcgatcga
     2101 ggcctcgcac ctgatgaagg acggcgactg gcgcccgcag ctcgacgagt tgtacgcgct
     2161 cggcgtgcgc acgctgcagc cggtgcacca gctcgacaac cgcttcggcg gggccgcgcc
     2221 gcacaacagc atcttccacg tcgcgcagta cgccgagagc tgtcacatcg acgaggactg
     2281 cggcctgacc accagcacgc tgacgctcgg cttcgacgtc gacgccgact gcaagaacac
     2341 gctcggcctg accgacgacg gcgaggcgct ggtggcggag atgatggacc gcggcatgct
     2401 cgtcgacgta gcccacctgt cggagcgcgg gatcaaggac gtgcacgacc tcgccgtcgc
     2461 ccgcgagcac tacccgatct acgtctcgca cgggcacttc cgcgagatca tgacgaagga
     2521 gcggcagcgc gaggagaaga cgacgccggc ctgggtgatc gagatccttc gtgagaccgg
     2581 cggcatgatc gggctgcgca ccggccacga ggaggtcaac agctatgacg cctccgcggt
     2641 cgcgaacacc tgccacggat cgagccggtc gttcgcgcag gcctatgact tcggacgcct
     2701 cggcctcaag gtcgcgatcg gcctcggatc cgacctcaac ggcttcatcc agcagacccg
     2761 cccgcgcttc ggcgcggccg cctgctcggc gtcgttcacc accgaggcgc agtgccaggc
     2821 ccgcgagcag cgcgaggccg gcccgccgcc gctgggcaac gacctcgacg aggccggcct
     2881 cggacacgtc ggcctgctcc cggcgctgct cgccgacctc gaccagctcg gcacggacac
     2941 ggcgccgctg cgcagctcgg ccgacgactt cgtgcgcatg tgggagcgcg ccgcgagccc
     3001 gcgctcggga ccagccgcga acgcggacga cctcgatgtc tccggcgtca cggtgctgcc
     3061 ggtgcacagc ctgcgcgagg ccgagttccc ggcgcagtgc ggcgactcgt actgcgcggg
     3121 cgcgctcgag gccggcgcgg agtgtcgctt cgccgcggag tgcgtcagcg gggagtgcgt
     3181 cggcgccggc gagtgcggca agccgcgggg cgtttgcgcc gggtagcgga cttctcgggc
     3241 ccgcagatcc gcagtagcgt gccgcggatg acgatccttg tcaccggtgc cagcggtcag
     3301 gtcggcggcg cgttgcgagc cgcgctcgac gcccgaaaca tcccgtggat cgggctgtca
     3361 cgacggcgac ccgccggcgc gcaggggcag tggcgatacg gcgacttcga ggagccgagc
     3421 tcgctg
//
LOCUS       DASWNJ010000867         5664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_441361, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000867 DASWNJ010000000
VERSION     DASWNJ010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5664
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441361"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            443..1675
                     /locus_tag="VGB85_14745"
     CDS             443..1675
                     /locus_tag="VGB85_14745"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY0135339.1"
                     /translation="MPPTDPGVLRGAQSLPLDAIVRHAKDNAMRVRVSRARLQLGEAA
                     VEGAKPLLPDNPQLYVGIGRRWNNFGHNFELQSTLTQPIEIGGERGLRIKSGRRYRDY
                     LDKELAQTQWEVYANVHYAYNMALLARERAVTAERTLEFSTRLLEVAQRRAQAGEISS
                     LRVRIATGELAQARQAKLSADLEYRLACIHLSEMAGWPPGQLVTPAGGLPAPIHASTA
                     TDLIARVQQEHPALRAREAAVHLGNARVSSAERDRLPEPQIGVYFGREYEPGAMTGAS
                     KIALFMFTLPLPLWKRNQANRAQSKAELSVAEQELEATRYGLALSALRAVDALNTAAE
                     RVRTYSSEVVPRFQENLDLLQRAFELGEVDILEVFVARENFMRIQAEALDAYRSYFDA
                     EYTLETILGSPLQAIATG"
     gene            1702..2469
                     /locus_tag="VGB85_14750"
     CDS             1702..2469
                     /locus_tag="VGB85_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135340.1"
                     /translation="MRRSPARSRWLAVLVSVAIVAPPASALAQTPAIRPAPAGAYDPS
                     ATAAYTEAQGLVRGRDYSEAVARLDLAIDLEPSWPAPVELRAEAFGALAERYAPSEAY
                     LSAQASDLERLVALEPGPEAAGRQQQAAVLRRQAKEAREVEQKRRKLVKPAMLVITAS
                     GALIASGAFMLGFIPATSSDAYGQRRYITAGATMLALGVALAVPAITLGVLAGRQGKR
                     DSALSDFNVRTDRPQADLAVAPQTLPGGGGMALRLRF"
     gene            2482..3000
                     /locus_tag="VGB85_14755"
     CDS             2482..3000
                     /locus_tag="VGB85_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135341.1"
                     /translation="MRAPLRTLSCALLGASVGLGSPVARAADDERAHQELEARTKLDL
                     DGLWQEYKRKGDARTFEQFVEAKYRRKRDVGRGLVFGGASLGVVAAAMFFLALPRNDA
                     APVTYTSYGVMGLSVGMMIAGGVMWRRNFRRLESIENTGYALSSRGRVRLLSAGPVAL
                     PRGGGVSFGLAF"
     gene            complement(3013..3903)
                     /locus_tag="VGB85_14760"
     CDS             complement(3013..3903)
                     /locus_tag="VGB85_14760"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY0135342.1"
                     /translation="MHRVVLTAVLLVGLPGCAPPRPEHCSSEAAGCPRSLYAMDVPTL
                     SRVLQRGQVRVLDLRDVSEYTAGHIPGATHLDPESLRATVGGLPGQVAPADRVGAVLA
                     AAGVAAGDSVVVYDADNGPAAARTLWTLLYYGWSSGRVHVLDGGFVAWTTMDGSLVPG
                     EPVRINKRAVHLATPTNERRVSAAWVSGHLGDPKVALLDARTAEEYAAGHIPGAVHLP
                     WQSTRTGTTFLADDAMLDLYGKTFDAATVIVYDDTGLRASVLWLTLKMLAHPDVRIYD
                     GSWSEWSARADLPKTTGTSP"
     gene            4139..>5664
                     /locus_tag="VGB85_14765"
     CDS             4139..>5664
                     /locus_tag="VGB85_14765"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0135343.1"
                     /translation="MLYRLIALCIQRRLAVIAVTLMIAVFGVYAYLRTPVEAYPDVTN
                     VQVEVVAQVSGLAPEEMERQVTIPLERALNGNPGMIQMRSESGFGLTIIWCVFEDGMD
                     SFRARTLTAERLLTAEVPEEAFVRLTPDATPLGKIYYYRLVSDRHDLYQLRSEQEWTV
                     ARILKQVPGVADAVGMGGFLKEMHVEVDPAKLASYGLTLRDVTDAVEHSNLNVGGGFQ
                     RRGDQQFMIRGIGYLMSPQDIKDIVLAVDEGTPVTIRDVARVVQSYTPRQGAVGYNEE
                     REIVEGIVLLRRGENPEEVLDGIHEKVDYLNQHILPEGMRIEPMYDRSTLTGLTLHTV
                     HENMLHGFLLIVGVVWLFLRTVRGSLLVAVVIPLSLLTAFAGLYLIDLPANLISMGAI
                     DFGILVDGAVVLVESAVHEGRAVRPTSKREMLELVGRSTLRVARPTFFAMAVIIAALI
                     PVFTLQSVEGRIFRPLALTYSFALIGALVFSLTVVPALCAVFMRPKDAATNEPKSLLK
                     L"
BASE COUNT          811 a         2003 c         1999 g          851 t
ORIGIN      
        1 atactccagc accgggccaa gtcaagagat gacactcacg tcgaaaaacc ccgttcaacg
       61 cgtccgggga cgggcgctgc cgcctgagca cacaaggcca catgttacac tcggcgcgct
      121 tctcgaccgg ccgggccagc cgccgaaaag acccccggtc gccccggctg gccccaccgt
      181 ttcggcctcc gtttttgctg ccggcttgcc tccgttctcg ccccgtttcg tcatgaacca
      241 tcgatctcgt gcggtcacca gaatgctgcg ccttcgggct gccgcccggg cgggcgccgt
      301 gctcgcaacg acgacgacct tgctcgcgtc tctcgtgcag gccgctccgg cgcccgccag
      361 agcgcgcccg gcgacgacgc gaagcgacgc ccccccgccg ttgctcacac ctgcgccgcc
      421 cgcctctccg gccaaagacg tggtgccgcc gacggacccc ggcgtgctgc gcggcgcgca
      481 gtcgttgccg ctcgacgcga tcgtgcggca cgccaaggac aacgcgatgc gggtgcgtgt
      541 gtcgcgggcg cggctccagc tcggcgaggc agcggtcgag ggtgccaagc ccctgttgcc
      601 cgacaacccc cagctgtacg tcggcatcgg ccggcgctgg aacaacttcg ggcacaactt
      661 cgaattgcag agcacgctga cccagccgat cgagatcggc ggcgagcgcg gcctgcggat
      721 caagtcgggc cggcgctacc gcgactacct cgacaaggag ctcgcgcaga cccagtggga
      781 ggtctacgcg aacgtccact atgcatacaa catggccctg ctcgcgcgag agcgagcggt
      841 cacggcggag cgcacgctcg agttctcgac gcggctcctc gaggtggcgc agcgccgggc
      901 ccaggccggc gagatctcca gcttgcgcgt gcggatcgcc accggcgagc tggcccaggc
      961 gcggcaggcc aagctcagcg cggacctcga gtatcggctc gcgtgcattc acctcagcga
     1021 gatggccggc tggcctccgg gacagctggt cacgccagcg ggtggcttgc cggcgccgat
     1081 ccacgcgagc actgcgaccg acctgatcgc ccgcgtgcag caggagcacc cggccctgcg
     1141 cgcccgcgag gccgcggttc acctcggcaa cgcccgcgtc agctcggccg agcgcgaccg
     1201 cctgcccgag ccgcagatcg gcgtgtactt cggccgcgag tacgagccgg gcgcgatgac
     1261 cggcgcctcg aagatcgcgc tgttcatgtt cacgttgccg ctgccgctgt ggaagcgcaa
     1321 ccaggccaac cgcgcccaga gcaaggccga gctgtcggtc gccgagcagg agctcgaggc
     1381 gacccgctac ggcctggcac tcagcgcgct gcgggcggtc gacgccctca atacggcggc
     1441 cgagcgcgtg cgcacctact cgagcgaggt cgtgccgcgc ttccaggaga acctcgactt
     1501 gcttcagcgg gccttcgagc tcggcgaggt cgacatcctc gaggtgttcg tcgcgcgcga
     1561 gaacttcatg cgcatccagg cggaggcgct cgacgcctat cgctcgtact tcgacgcgga
     1621 atatacgctg gagacgatcc tcggcagccc gctgcaggcg atcgctaccg gctaaagggc
     1681 gtgttgatgt actatccggt catgcgccgc agtcccgccc gctcacgctg gctcgctgtc
     1741 ctcgtgtccg ttgcgatcgt cgccccccct gcgtcggcgc tggcccagac gccggcgatc
     1801 cggccggcgc ccgcgggcgc gtacgacccg tcggcgacgg cggcgtacac cgaggcgcag
     1861 gggctggtgc gcggccgaga ttacagcgag gcggtcgccc gcctcgacct tgcgatcgac
     1921 ctcgagccga gctggccggc gccggtcgag ctgcgcgcgg aggccttcgg cgcgctggcg
     1981 gagcgctatg cgccgagcga ggcgtacctg tcggcgcagg cctcggacct cgagcggctg
     2041 gtcgcgctcg agcccgggcc cgaggcggcg gggcgccagc agcaggccgc ggtgctgcgg
     2101 cgccaggcca aggaggcccg cgaggtcgag cagaagcggc gcaagctggt caagccggcg
     2161 atgctcgtga tcacggcgag cggcgcgctg atcgccagcg gggcgttcat gctgggcttc
     2221 atccccgcga cgtcgagcga cgcctacggg caacgccgct acatcaccgc gggcgcgacg
     2281 atgctggcgc tgggcgtcgc cctggcggtc ccggcgatca cgctcggcgt gttggccggg
     2341 cggcagggca agcgcgactc ggcgctctcc gacttcaacg tgcgcaccga ccggccgcag
     2401 gcagacctcg ccgtcgcgcc gcagacgctg cccggcggcg gcggcatggc cctgcggctg
     2461 cgcttctagt atcctcgccg cgtgcgcgcg ccgcttcgta ctttgtcctg cgccctcctc
     2521 ggcgcgtccg tcggtctcgg cagcccggtc gcccgcgctg cggacgacga gcgggcgcat
     2581 caggagctcg aggcgcggac caagctcgac ctcgacgggc tgtggcagga gtacaagcgt
     2641 aagggcgatg cgcggacctt cgagcagttc gtcgaggcca aatatcgccg caagcgtgac
     2701 gtcggccgcg gcctggtgtt cggcggcgcg agcctcggcg tggtcgcggc ggcgatgttc
     2761 ttcctcgccc tgccccgcaa cgacgccgcg ccggtgacct acacgtccta cggggtcatg
     2821 ggcctgtccg tcggcatgat gatcgcaggt ggggtcatgt ggcgcaggaa ctttcgccgg
     2881 ctcgagagca tcgagaacac cggctatgcg ctcagctcgc gcgggcgggt gcgcctgttg
     2941 tcggccggcc cggtcgcgct gccgcgcggc ggcggcgtca gcttcggcct cgcgttctga
     3001 cgctcaccgc ggtcacgggc tcgtgccggt ggtcttcggc aagtcggcgc gggcgctcca
     3061 ctcggaccac gagccgtcgt agatgcgcac gtccgggtgc gcgagcatct tgagcgtcag
     3121 ccacagcacg ctcgcgcgca ggccggtgtc gtcgtagacg atgaccgtcg cggcgtcaaa
     3181 ggtcttgccg tagaggtcga gcatcgcgtc gtcggcgagg aaggtcgtgc cggtgcgcgt
     3241 cgactgccag ggcaggtgga cggcgcccgg gatgtggccc gcggcgtact cctccgccgt
     3301 gcgcgcgtcg agcagggcga cctttggatc gccgaggtgc ccgctcaccc acgcggcgct
     3361 gacgcggcgc tcgttggtcg gcgtcgccag gtgcacggcc cgcttgttga tccgaaccgg
     3421 ctcgccgggc accagcgaac cgtccatcgt cgtccaggcg acaaagccgc cgtcgagcac
     3481 gtgcacgcgc ccggacgacc agccatagta cagcagggtc cacagggtgc gcgcggcggc
     3541 gggtccgttg tcggcgtcgt agacgacgac gctgtcaccc gcggcgacgc cggcggcggc
     3601 gagcacagcg ccgacgcgat cggccggcgc gacctgtccg ggcaggccgc cgacggtggc
     3661 gcgcagcgac tcggggtcga ggtgcgtggc gccggggatg tggcctgccg tgtactcact
     3721 gacatcacgc aggtcgagga cccgcacctg tccgcgctgg agcacgcgcg agagggtcgg
     3781 gacgtccatc gcgtagaggc tgcgcgggca gccggcggcc tccgacgagc aatgttcggg
     3841 ccgcggtggc gcacagccgg gcaggcccac gaggaggacg gcggtgagga cgacccgatg
     3901 catcgcgaat caacccgcaa gatcgcccag cctcggcgaa tccgcaagta tttgtgcgca
     3961 tgccggcatg actcccctcg ctgcctggtt gtaacggcaa cttgcgcggc gacgcgtgca
     4021 cgacctatgc tcccgcggcc gctggcgtgc gctctaacgt tgtaacgttc gacgcgtaac
     4081 gctgtaccgc cggcctcctc gacagctccc gtcgcccgcc ccgaagtctc ttccgaccat
     4141 gctctaccgg ctcatcgcgc tctgtattca gcgtcgcctg gcggtgatcg ccgtgacctt
     4201 gatgatcgcg gtgttcggcg tctacgccta cctgcgcacc ccggtcgagg cgtaccccga
     4261 cgtgaccaat gttcaggtcg aggtcgtcgc ccaggtgtcg ggtctggcgc cggaggagat
     4321 ggagcgtcag gtgacgatcc cgctcgagcg ggcgctcaac ggcaacccgg gcatgatcca
     4381 gatgcgaagc gagagcggct tcgggttgac gatcatctgg tgcgtgttcg aggacgggat
     4441 ggacagcttc cgggcgcgca cgctgaccgc ggagcgcctg ctgaccgcgg aggtcccgga
     4501 ggaggcgttc gtgcggctga cgccggacgc gaccccgctc ggcaagatct actactaccg
     4561 gctggtcagc gaccgccacg acctctacca gctgcgctcg gagcaggagt ggaccgtcgc
     4621 gcgcatcctc aagcaggtgc ccggggtcgc cgacgcggtc ggcatgggcg gcttcctcaa
     4681 ggagatgcac gtcgaggtcg acccggccaa gctggcgtcg tacgggctga cgctgcgcga
     4741 cgtcaccgac gcggtcgagc actcgaacct caacgtcggc ggcggcttcc agcgccgcgg
     4801 cgatcaacag ttcatgatcc gcgggatcgg gtacctgatg tcgccgcagg acatcaagga
     4861 catcgtgctc gcggtcgacg agggcacgcc ggtgacgatc cgcgacgtcg cccgggtggt
     4921 ccagtcgtac acgccgcggc agggggcggt cggctacaac gaggagcgcg agatcgtcga
     4981 gggcatcgtg ctcctgcgcc gcggcgagaa cccggaggag gtgctcgacg ggatccacga
     5041 gaaggtcgac tacctcaacc agcacatctt gcccgagggc atgcgcatcg agccgatgta
     5101 cgaccgcagc accctgaccg ggctgacgct gcacaccgtg cacgagaaca tgctgcacgg
     5161 ctttttgctg atcgtcgggg tcgtgtggct gttcctgcgc acggtgcgcg ggtcgctgct
     5221 ggtcgccgtc gtcatcccgc tgtcgctgct gacggcattc gccgggctgt acctgatcga
     5281 cctgccggcc aacctgatct cgatgggcgc gatcgacttc ggcatcctcg tcgacggcgc
     5341 cgtcgtgctg gtcgagagcg cggtccacga gggccgcgcg gtccggccga cgagcaagcg
     5401 cgagatgctc gagctggtcg gccgctcgac gctgcgggtc gcccggccga ccttcttcgc
     5461 tatggcggtc atcatcgccg cgctgatccc cgtgttcacg ctgcagagcg tcgagggccg
     5521 catcttccgc ccgctcgcgc tgacctacag cttcgccctc atcggcgcgc tggtgttctc
     5581 gctgacggtc gtgcccgccc tgtgcgcggt gttcatgcgg cccaaggacg cggcgaccaa
     5641 cgagccgaag tcgctgctca agct
//
LOCUS       DASWNJ010000868         2375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_686371, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000868 DASWNJ010000000
VERSION     DASWNJ010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686371"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1905
                     /gene="mutS"
                     /locus_tag="VGB85_14770"
     CDS             <1..1905
                     /gene="mutS"
                     /locus_tag="VGB85_14770"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEY0135344.1"
                     /translation="LDETSLRNLEIFKTLRDGQRKGSLLWAVDATRTAMGARMLREWL
                     GAPLRTLPAIAARHQAIEALIAEPRLRKALQERLKDVRDIVRLAARARLGTATPRELG
                     ALRASLAALPGVHGLLCELARARLPEPTGPEGQVVRAPCAGEGDLTRLPVLLDLGGDL
                     LVDVHAELARVLVDEPPAHTRDGGMVRPGADAELDRQHALRDGGREALAAIEARERER
                     TGIANLRVQHNRVFGYYIEVMKSQLTRVPADYIRKQTMTNAERYVTPELAEHETAVLG
                     AQSLALEREQTLFLALREQVSAAGQRLCQVGEALARLDVLAGLAEVAETHGHVRPELT
                     DEPVLEIEEGRHPVVERMLGAGRFVPNDLGVRANAGAVGGLARFLLVTGPNMGGKSTV
                     MRMAALITILAQVGAYVPAVRARVGVVDRIFTRVGAADDLGRGDSTFMVEMRETAAIL
                     SQATPRSLVLLDEIGRGTATFDGLALAWAITEFIHDQIGCRALFATHYHELTALSDKL
                     AGLRNVHVAVHEDRGTIVFLHRVEAGPAEHSYGIQVGRLAGLPASVLRRAHKLLARLE
                     ASDRGVVPQLDLFNRAHNEAEADPQQLAHESVLADLRDLHPDELSPRAAHEALRQLHE
                     RLLRIETAPV"
     gene            1939..>2375
                     /locus_tag="VGB85_14775"
     CDS             1939..>2375
                     /locus_tag="VGB85_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0135345.1"
                     /translation="MPPRRAPADASPLDLFHEPVRRWFRAALGEPTAAQGKAWPAIAA
                     GRSTLLLAPTGSGKTLAAFLAGLDRLMFSPEPPPKERCRLLYISPLKALAVDIERNLR
                     APLAGIAAVAQQLGAEHRIPTVGVRSGDTPAIERARMIRNPPD"
BASE COUNT          324 a          817 c          928 g          306 t
ORIGIN      
        1 ctcgacgaga cctcgctgcg caacctcgag atcttcaaga cgctgcgcga cgggcagcgc
       61 aaggggagct tgctgtgggc ggtcgacgcg acgcgcaccg cgatgggcgc ccggatgttg
      121 cgcgagtggc tcggggcgcc gctgcgcacg ctgccggcga tcgcggcgcg gcaccaggcg
      181 atcgaggcct tgatagcaga gccgcggctg cgcaaggcgc tgcaggagcg gttgaaagat
      241 gtgcgagaca tcgtccggct ggccgcgcgg gcgcgcctcg gcacggcgac gccgcgcgag
      301 ctgggggcgc tgcgcgcctc gctggcggcg ctgccgggcg tgcatgggct gctgtgcgag
      361 ctggcgcgcg cgcgcctgcc cgaaccgaca ggtcccgagg gacaggtggt gcgggccccg
      421 tgcgccgggg agggcgacct gacgcgcttg ccggtcctgc tcgacctcgg cggcgacctg
      481 ctggtggacg tgcacgccga gctggcgcgg gtgttggtcg acgagccgcc ggcgcacacg
      541 cgcgacggcg ggatggtgcg cccgggcgcc gacgcggagc tcgaccgcca gcacgcgctg
      601 cgtgacggcg ggcgcgaggc cctggcggcg atcgaggccc gcgagcgcga gcggaccggc
      661 atcgccaacc tgcgggtcca gcacaatcgc gtgttcggct actacatcga ggtgatgaag
      721 tcgcagctga cgcgggtgcc cgccgactac atccgcaagc agacgatgac caacgcggag
      781 cgctacgtga cgcccgagct ggcggagcac gagacggcgg tgctcggggc gcagagcctc
      841 gcgctcgagc gcgagcagac gctgttcctg gcgctgcgcg agcaggtcag cgcggcgggg
      901 cagcgcctgt gtcaggtggg cgaggcgctg gcgcggctcg acgtgctcgc ggggctggcg
      961 gaggtcgccg agacgcacgg gcacgtgcga cccgagctga cggacgagcc agtgctcgag
     1021 atcgaggagg gccggcaccc ggtcgtcgag cgcatgctcg gcgcgggtcg cttcgtgccc
     1081 aacgacctcg gcgtgcgcgc caacgcgggg gcggtcggcg ggctggcgcg cttcctgctg
     1141 gtcaccgggc ccaacatggg cggcaagagc acggtgatgc gcatggcggc gctgatcacc
     1201 attttggcgc aggtgggggc gtacgtgccg gccgtgcgcg cgcgggtggg ggtggtcgac
     1261 cggatcttca cgcgcgtcgg cgcggcggac gacctcgggc gcggcgacag cacgttcatg
     1321 gtcgagatgc gcgagacggc ggcgatcctc agccaggcga cgccgcgatc gctggtgctg
     1381 ctcgacgaga tcggccgcgg cacggcgacc ttcgacgggc tcgcgctcgc gtgggcgatc
     1441 accgagttca tccacgacca gatcggctgc cgggcgttgt tcgcgaccca ctaccacgag
     1501 ctgaccgccc tgtcggacaa gctcgccggg ctgcgcaacg tccacgtggc ggtgcacgag
     1561 gaccgcggca cgatcgtgtt cctgcaccgc gtcgaggccg ggccggcgga gcacagctac
     1621 ggaattcagg tcggacgtct cgcgggcctg ccggcgtcgg tgctgcggcg ggctcacaag
     1681 ctcctggcgc ggctcgaggc gagtgaccgc ggagtggtgc cgcagctcga cctgttcaat
     1741 cgagcccaca acgaggccga ggcggatccg caacagctcg cccacgagtc ggtgctcgcc
     1801 gatctgcgcg acctgcaccc cgatgagctg agcccacggg ctgcgcacga ggccttgcgc
     1861 caattgcacg agcgactgct gcggatcgag actgcaccgg tgtagcagca gacaggcgcg
     1921 gctctgctaa ggtggaacgt gccgccccgc cgcgcccccg ccgacgcctc cccgctcgac
     1981 ctgttccacg agccggtccg gcggtggttt cgggccgcgc tcggcgagcc gacggcggcg
     2041 cagggcaagg cctggccggc gatcgccgcc ggtcgctcga cgctgttgct ggccccgacg
     2101 ggatccggca agacgctggc ggcgtttttg gccgggctcg accggctgat gttctcaccc
     2161 gagccgccgc cgaaggagcg ctgccggctg ctgtacatct cgccgctcaa ggcgctggcc
     2221 gtcgacatcg agcgcaacct gcgggccccg ctggcgggca tcgccgcggt cgcgcagcag
     2281 ctcggggcgg agcaccggat cccgacggtc ggggtgcggt ccggcgacac gccggcgatc
     2341 gagcgggccc gcatgatccg caacccgccg gacat
//
LOCUS       DASWNJ010000869         1671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_399155, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000869 DASWNJ010000000
VERSION     DASWNJ010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399155"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VGB85_14780"
     CDS             <1..496
                     /locus_tag="VGB85_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135346.1"
                     /translation="VEFLFTANPGEPLAPLTKVASGGELSRVLLAVKGVLATGDHVTT
                     YVFDEVDSGVGGAVAEAIGRRLRAASQQRQVLCITHLPQIAAFADAHFRVEKHTKDGR
                     TITRVVRLSDEARVEEIARMLGGSRVTGTAREHASELVAEAQRAIRKPAARGKPRAAA
                     RGSA"
     gene            complement(588..>1671)
                     /locus_tag="VGB85_14785"
     CDS             complement(588..>1671)
                     /locus_tag="VGB85_14785"
                     /inference="COORDINATES: protein motif:HMM:NF015657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135347.1"
                     /translation="GCHRDGGIGSFTMQDVEVAQDYAGAMKQSTATREMPPWMPDPAC
                     QNFHDARILSDAEIALFAAWADGGALAGDPADAPTPVPPPSLGKIDIELQASAPYTPN
                     KGLGEDDYRCLVLDPKQGAPLSLVGVNFLPDQANMVHHVVLFAVDRQTAATKDASEPG
                     LGWTCFGGPGTPGGMNTLGAWVPGSPPLMYPQGTGLTLLASEVIVMQMHYNFENLGGA
                     DPKPDQTKVQLQYAAGPVTTAKMIPLIDMGFNIPPSSMGYTHGTSLTLQKSTRLYGML
                     PHMHKLGRQISLTSSALGCMIDIPEWDFHWQQFYFYENQQGMTLPAKSKLDLKCTWDN
                     PGGQAVGWGEGTADEMCVAFIYTTTP"
BASE COUNT          232 a          534 c          641 g          264 t
ORIGIN      
        1 ggtcgagttc ctgttcacgg ccaaccccgg cgagccgctg gcgccgctga cgaaggtggc
       61 gtcgggcggc gagctgtcgc gcgtgttgct ggcggtcaag ggcgtgctgg cgaccggcga
      121 ccacgtgacg acgtacgtgt tcgacgaggt cgactcgggg gtcggcggcg cggtggccga
      181 ggcgatcggc cggcgcctgc gggccgcgtc gcagcagcgt caggtgctgt gcatcaccca
      241 cctgccgcag atcgcagcct tcgcggacgc gcacttccgg gtcgagaagc acaccaagga
      301 cggccggacg atcacgcggg tggtgcggct cagcgacgag gcgcgcgtcg aggagatcgc
      361 gcgcatgctc ggcggctcgc gggtcaccgg cacggcgcgc gagcacgcca gcgagctggt
      421 cgccgaggcc cagcgagcga tacgcaagcc ggccgcgcgc ggcaagcccc gcgcggcggc
      481 ccgcggctcg gcctgagccg ggagccagcg agccgggact gtcggcgccg cggctttcga
      541 cgcagcggct gtcggcgcga ctgcttcgag ccggcgccgg cgcgggctca gggcgtcgtc
      601 gtgtagatga aggcgacgca catctcgtcc gcggtgccct cgccccagcc gacggcctgt
      661 ccgccggggt tgtcccacgt gcacttgagg tcgagcttcg acttggcggg cagcgtcatg
      721 ccttgctggt tctcgtaaaa atagaactgc tgccagtgga agtcccactc ggggatgtcg
      781 atcatgcagc cgagcgcgga gctcgtgagc gagatctggc ggccgagctt gtgcatgtgc
      841 ggcagcattc catacagccg cgtcgatttt tgcaaggtca gcgaggtgcc gtgggtgtag
      901 cccatcgagc tcggcgggat attgaagccc atgtcgatga gcgggatcat cttggcggtg
      961 gtcaccgggc cggcggcgta ctgcagctgc accttggtct ggtccggctt ggggtcggcg
     1021 ccgccgagat tttcaaaatt gtagtgcatc tgcatgacga tgacctcgct ggcgagcagc
     1081 gtcaggccgg tgccctgcgg gtacatcagc ggcggcgagc cgggcaccca ggcgccgagc
     1141 gtgttcatgc cacccggcgt gccgggaccg ccgaagcagg tccagccgag gccgggctcg
     1201 ctcgcgtcct tggtggccgc ggtctggcgg tcgacggcga acagcacgac gtggtggacc
     1261 atgttggcct ggtccgggag gaagttgacg ccgacgagcg agagcggggc gccctgcttg
     1321 gggtcgagca cgaggcagcg atagtcgtcc tcgccgagcc ccttgttcgg ggtgtagggc
     1381 gcgctggctt gcagctcgat gtcgatcttg ccgagcgagg gcgggggtac cggcgtgggc
     1441 gcgtcggcgg ggtcgcccgc gagcgcgccg ccgtcggccc acgcggcgaa caacgcgatc
     1501 tcggcgtcgc tcaggatgcg cgcatcatga aaattctggc acgcggggtc gggcatccat
     1561 ggcggcatct cgcgcgtcgc ggtggattgc ttcatcgcgc cggcgtagtc ctgcgcgacc
     1621 tcgacgtctt gcatggtgaa cgagccgatg ccgccgtcgc ggtggcaacc c
//
LOCUS       DASWNJ010000870         2815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_368484, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000870 DASWNJ010000000
VERSION     DASWNJ010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368484"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="VGB85_14790"
     CDS             complement(<1..1076)
                     /locus_tag="VGB85_14790"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0135348.1"
                     /translation="MEIRFTSSKQVRTSADAELPLPVVRAPDLSGRVLGARYSLRRRI
                     GGGSLGTVYEARDMTLGTAVAVKVLHPDYSGDEEFRRRFHQEARLGARLRHEHSVAVT
                     DLGQSDDGLLYSVMEYLEGESLDGLLSSRPGPLPWRRVVTIAVQVCAALQAAHDRGVI
                     HRDIKPGNCFLVRREGHRGQAGQETEDAPPFTFVKVLDLGLARLMPATGRPTPPGRLG
                     APEYLAPEQIQGFTCDHRVDIYALGVMMYKLLTRRLPFTGDNPAAIMRMHIEAVPTPP
                     RRLAPSADIPASLEAVVLRALSKDRGDRYPSAHMMAQAIRAAAADAEAEAIAIDEAPD
                     DTATTLWRTGLGASTTRGTQVSAT"
     gene            complement(1409..2626)
                     /locus_tag="VGB85_14795"
     CDS             complement(1409..2626)
                     /locus_tag="VGB85_14795"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HEY0135349.1"
                     /translation="MMSSLSKLAGPGLVVSFVLASCGDDGRESEGMVNTNVATGIATL
                     PGDSSVGTTGMTGATDGTATDPGSADSDPVTTTQGEGSTAPKFDLGDPPETTMSPDSG
                     DVDCTIDPNQEGCKCSIPDHAPCDAGTQDPFNAMGLNCPGELQVNATKNGDGLAFGVR
                     NSFGQAGVFNPREGTQYAVIGSGVVAELNQTTPGGDSNQNPSLCNDILGGSQDPGPNL
                     PAPLKTNAVGGDCQQNPGLVGTGDCSSTVQDQFQQGMNAFDYTELRFVLQVPPGVVSL
                     SYDFAFFSTEYPHYYGEQYNDMYIGWLESEKWTGNISFDAMGNPISLNAGFLDHKDDG
                     GNLPALAGTCMRQHAGTNWLQTTAGVTPGETITVVFAVFDLYDPVLDSYVFLDNFKWG
                     CNPVGKPQTMPPG"
BASE COUNT          391 a          919 c         1054 g          451 t
ORIGIN      
        1 atcgtcgccg agacctgggt gccgcgcgtg gtcgaggcgc cgaggccggt gcgccacagg
       61 gtggtcgccg tgtcgtcggg cgcctcgtcg atcgcgatcg cctcggcctc cgcgtcggcg
      121 gccgcggccc ggatcgcctg cgccatcatg tgcgcgctcg ggtagcggtc gccgcggtcc
      181 ttggacagcg cgcgcaggac cacggcctcg aggctggccg ggatgtcggc cgagggcgcg
      241 agccgccgcg gcggggtcgg cacggcctcg atgtgcatgc gcatgatcgc cgccgggttg
      301 tcgccggtga acggcaggcg gcgcgtcagc agcttgtaca tcatcacgcc gagcgcgtag
      361 atgtcgacgc ggtggtcgca ggtgaagccc tggatctgct ccggcgcgag gtactcgggc
      421 gcgccgaggc gacccggcgg ggtcggccgt ccggtcgccg gcatcagccg cgcgaggccg
      481 aggtcgagca ccttgacgaa ggtgaagggc ggcgcgtcct cggtctcctg gccggcttgc
      541 ccgcggtggc cctcacgccg caccaaaaag cagttgccgg gcttgatgtc gcggtgaatc
      601 acgccgcggt cgtgcgccgc ctgcaaggcc gcgcacacct gcacggcgat cgtgacgacg
      661 cgccgccagg gcaggggacc cgggcggctc gagagcaggc cgtcgaggct ctcgccctcg
      721 aggtactcca tcaccgagta gagcaggccg tcgtcgctct ggccgaggtc ggtgacggcg
      781 acgctgtgct cgtgtcgcag gcgcgcgccg aggcgggcct cctgatggaa gcggcggcga
      841 aactcctcgt cgccgctgta gtccgggtgc agcaccttga cggcgacggc tgtcccgagg
      901 gtcatgtcgc gcgcctcgta gacggtgccg aggctgccgc cgccgatccg ccggcgcaag
      961 ctgtagcgcg ccccgagcac gcgacccgag agatcgggcg cacgcacgac gggcagcggg
     1021 agctcggcgt cggccgaggt ccggacttgc ttgctcgagg tgaaacggat ctccatggac
     1081 cgggcgggcg cggtcagggc ctacttcgca cgcccgatcg agattccatg gatcagcaag
     1141 gaagtccaca gcggccgatc cgaccgcgtt aggttggcgc ccgcattggc caatgccttt
     1201 tatgaattcc agctaacaat tgtttcactg accgctgtca cgagggccgc cagagcccgc
     1261 gccgcgccgg cggtccgggt tccaggcccg gccgtgtgcg gccttgtgat gatgttcaca
     1321 tccgcgcggc ggggccgacg cgcatgagac agcgaggaga cagcgaggag acagcgagga
     1381 gacagcgagg ggacagcgag ggaggacgtc agcccggggg catcgtctgg ggcttgccga
     1441 ccgggttgca gccccacttg aagttgtcga ggaacacgta gctgtcgagg acggggtcgt
     1501 agaggtcgaa cacggcgaag acgacggtga tcgtctcgcc cggggtcacg ccggccgtgg
     1561 tctgcaacca gttggtgccg gcgtgctggc gcatgcaggt gccggcgagc gcgggcaggt
     1621 tgccgccgtc gtccttgtgg tcgaggaagc cggcgttgag cgagatcggg ttgcccatcg
     1681 cgtcgaagga gatgttgccg gtccacttct ccgactcgag ccagccgatg tacatgtcgt
     1741 tgtactgctc gccgtaatag tgggggtact cggtcgagaa gaacgcgaag tcgtagctca
     1801 gcgagacgac cccgggcggg acctgcagga caaagcgcag ctcggtgtag tcgaacgcgt
     1861 tcatgccctg ctggaactga tcctgcaccg tgctcgagca gtcgccggtg ccgaccaggc
     1921 cggggttctg ctggcagtcg ccgccgaccg cgttggtctt gagcggcgcc ggcaagttcg
     1981 gacccgggtc ctggctgccg ccgagaatgt cgttgcacag gctcgggttt tggttgctgt
     2041 caccgccggg ggtggtctgg ttgagctcgg cgacgacgcc gctgccgatc acggcgtact
     2101 gcgtgccctc gcgcgggttg aacacgccgg cctgaccgaa gctgttgcgc acgccgaacg
     2161 ccagaccgtc gccgttcttg gtcgcgttga cctgcaactc gccggggcaa ttgaggccca
     2221 tcgcgttgaa cgggtcctgg gtgccggcgt cacacggcgc gtggtccggg atgctgcact
     2281 tgcagccctc ctggttgggg tcgatcgtgc agtcgacgtc gcccgagtcc gggctcatcg
     2341 tcgtctcggg cgggtcaccg aggtcgaact tgggcgccgt gctgccctcg ccctgggtcg
     2401 tcgtcaccgg gtccgagtcg gcgctgcccg ggtcggtcgc ggtgccatcg gtggcgccgg
     2461 tcatcccggt ggtgccgacg ctcgagtcgc ccgggagcgt ggcgatcccg gtcgcgacgt
     2521 tggtgttcac catgccctcg ctctcgcgcc cgtcgtcccc acacgacgcg aggacgaacg
     2581 acaccacgag cccgggacca gccagcttcg agagagagga catcatgtcg ccaccgtacc
     2641 aacttcgccg cgcgggccaa tcggcaaatc tgcgcacggg ccggcgtgcc cttcctgggg
     2701 tctgccagcc tcgcgcgggg ctcgttgctt cgtgtgcggc gtgggggaag gcggcgtgac
     2761 ggcggcgtgg cgcagtcggc gttgcggtgg tgaaagtcac gctcgcccgg gcgcg
//
LOCUS       DASWNJ010000871         3085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_508772, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000871 DASWNJ010000000
VERSION     DASWNJ010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508772"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VGB85_14800"
     CDS             complement(<1..384)
                     /locus_tag="VGB85_14800"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEY0135350.1"
                     /translation="MKDIVSTVTALAAKLSERERQLLRAALTPAPEPIAIVGIGCRFP
                     GGADDPDSFWQLLDRGGDAVREVPKDRWDLADYFDPDPDAAGRMLTRHGAFLDRVDHF
                     DPGFFEISPHEARRMDPQQRLLLEVA"
     gene            complement(398..>3085)
                     /locus_tag="VGB85_14805"
     CDS             complement(398..>3085)
                     /locus_tag="VGB85_14805"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY0135351.1"
                     /translation="CWIDAAPTSPTIPPEPTRTPPPASLLRAIAWPPRPLAASEPLHK
                     TVWLAADPDDAHTLTRALARMHASVSLHAITDPPTGDIDAIVVLLAVTAPAMSVAASR
                     NFEGLWTLARAVLRRGDRDPPRLVIVTRGATDEAPAHAPAWGLGRALMHEHPALRCTL
                     IDLGPGPLPADQLAREIVTPDGEDQILLTDDQRRVARLAPAPSDPRTRPAAGRSFRLE
                     QDTPGVLNGLHLRAHPRTPPAPGHVEIAVEAAGLNFLDVLRALAVMPDDGTGLGAECV
                     GTVVALAADVDDLQLGDRVVALADGALGTHVVTPRALVVALPPGLDITAAATLPIATL
                     TAWQALHHVARLRAGERVLIHAAAGGVGQAAVQIALARGAVVFATAGTPAKRAMLTAQ
                     GVHHVSSSRDRSFVPAILDATAGEGVDVVLNSLAGELLAASLEVLRDDGRFIEIGKRD
                     YLEDRKIGLAPFLRRLTWSLVDLRGMMQDPSRLRPLLAEVAELWRSGVLRPLAHRVVP
                     ITSVHEAFTAMARGQHTGKLVIDLTHAAAAAIAPPRAPSFHDRPGVVLLTRATAITLH
                     VARTLIDLGAAHLVVLDPERTLTDHDLQILPRHVRVDRLRTLADLAGLTILAAIHTPP
                     ITPPSPLHASAPNIEPALAEAWQLHHWLAAHPVEHAIFCTHASATLGAPGQAPAAAID
                     CFLAALARHRRAHDLPALCIESDDLTPDEATLALRDCLARDTTHALALRLHPRRWRET
                     HLARARAPLLADLHDEASTNAPLRAQLLALDPRPRAEALQSHLLAQTAAVLRRDAARI
                     GLDDPLQQAGLDSLMALELRNRLESTLDLSLSPTVIWRHPTLRSLHRHLLELLEPPIV
                     EQVVIPDAPGSLTRTSAPDELAGIAAFLAEVAALADISP"
BASE COUNT          414 a          891 c         1378 g          402 t
ORIGIN      
        1 ggcgacctcg agcagcaggc gctgctgcgg gtccatgcgg cgggcctcgt gcggtgagat
       61 ctcgaagaag ccgggatcga agtggtcgac gcgatcgagg aaggcgccat ggcgagtcag
      121 catgcgaccg gcggcgtcgg ggtcgggatc gaagtagtcg gcgaggtccc agcgatcttt
      181 ggggacctcg cggaccgcgt cgccgccgcg atcgagcagc tgccaaaaac tgtcggggtc
      241 gtcggcgccg ccggggaagc ggcagccgat gccgacgatc gcgatcggct cgggcgcggg
      301 cgtgagggcg gcgcgcagga gctggcgctc gcgctcggag agcttcgccg cgagggcggt
      361 gacggtgctt acgatatctt tcatcaccaa gtcctgacta tggagagatg tcggcgagcg
      421 cggcgacctc ggcgaggaag gcggcgatgc cggcgagctc gtcgggcgcg ctggtccggg
      481 tgagtgaccc gggtgcgtcg ggtattacga cctgttcaac aattggtggc tcgagcaatt
      541 cgagcaggtg ccggtgcaat gagcggagcg tggggtgccg ccagatcacc gtcgggctca
      601 gtgagagatc gagggtcgac tcgaggcgat ttcgcagctc gagcgccatc agcgagtcga
      661 ggccggcctg ctgcaagggg tcgtcgaggc cgatgcgcgc cgcgtcgcgc cgcagcacgg
      721 cggcggtctg cgcgagcagg tgcgactgca gggcctcggc gcgcggccgt gggtcgaggg
      781 cgagcagctg ggcgcgaagc ggggcgttcg tggacgcttc gtcgtgcagg tcggcgagca
      841 gcggggcgcg ggcgcgggcg aggtgggtct ctcgccagcg tcgcggatgc aggcgcagcg
      901 cgagggcgtg ggtggtgtcg cgcgcgaggc agtcgcgcag ggcgagcgtc gcctcgtcgg
      961 gcgtgaggtc gtcgctctcg atgcacagcg cgggcaggtc gtgcgcgcgg cggtggcggg
     1021 cgagggcggc gaggaagcag tcgatcgcgg cggccggcgc ttgaccgggc gcgccgaggg
     1081 tggcggatgc gtgggtgcag aagatcgcgt gctcgaccgg gtgcgcggcg agccagtgat
     1141 ggagctgcca ggcctcggcg agcgcgggct cgatgttcgg cgcgctcgcg tgcagcggtg
     1201 atgggggggt gatcggcggc gtgtggatcg cggcgaggat cgtgaggccg gcgagatcgg
     1261 cgagcgtacg caggcggtcg acgcggacgt gacgggggag gatctgaagg tcgtgatcgg
     1321 tgagggtgcg ctcggggtcg aggacgacga gatgcgcggc gccgaggtcg atcaaggtgc
     1381 gcgcgacgtg cagggtgatc gcggtggcgc gggtcagcag cacgacgccg gggcggtcgt
     1441 ggaacgaggg ggcgcggggc ggggcgatcg cggcggccgc ggcatgggtc agatcgatga
     1501 cgagcttgcc ggtgtgctgg ccgcgggcca tcgcggtgaa ggcctcgtgg acgctggtga
     1561 tcgggacgac ccggtgcgcg agcgggcgca ggacgccgct tcgccacagc tcggcgacct
     1621 ccgcgaggag ggggcgcagg cgcgaggggt cctgcatcat gccgcgcagg tcgacgagcg
     1681 accaggtcag gcggcgcagg aacggggcga ggccgatctt gcgatcttca agatagtcgc
     1741 gcttgccgat ctcgatgaag cggccgtcgt cgcggaggac ctcgaggctg gccgcgagca
     1801 gctcgccggc gagggagttg aggacgacgt cgacgccctc gccggcggtc gcgtcgagga
     1861 tcgcggggac gaacgatcga tcgcgcgagc tactgacgtg gtgcacgccc tgcgcggtca
     1921 gcatggcgcg cttcgctggg gtgccggcgg tggcgaacac gacggcgccg cgggcgaggg
     1981 cgatctgcac ggcggcctgg ccgacgccgc cggccgcggc gtggatcagc acgcgctcgc
     2041 cggcgcgcag gcgagcgacg tggtgcaggg cctgccaggc ggtgagggtg gcgatcggga
     2101 gggtcgcggc ggcggtgatg tcgagcccgg gcgggagcgc gacgacgagg gcgcgcgggg
     2161 tgacgacgtg cgtgccgagg gcgccgtcgg cgagggcgac gacgcggtca cccagctgga
     2221 ggtcatcgac gtcggcggcg agggcgacga cggtgccgac gcactcggcc ccgagccctg
     2281 tgccgtcgtc gggcatgacg gcgagggcgc gtaacacatc gaggaagttg aggccggcgg
     2341 cctcgaccgc gatctcgacg tggccgggcg cgggcggggt gcgggggtgc gcgcgcaggt
     2401 gcagaccgtt gagcacgccg ggggtgtcct gctcgaggcg gaagctgcga ccggcggcgg
     2461 gccgcgtgcg gggatcgctg ggggcggggg ccaggcgagc gacgcggcgt tgatcgtcgg
     2521 tgaggaggat ctgatcctcg ccgtcggggg tgacgatctc gcgggcgagc tggtcggcgg
     2581 gcagcgggcc ggggccgagg tcgatcagcg tgcaacgcag ggccgggtgc tcgtgcatca
     2641 gcgcgcggcc gaggccccac gcgggggcgt gcgccggcgc ttcgtcggtc gcgccgcggg
     2701 tgacgatcac gaggcgcggt ggatcacgat cgccgcgacg caggaccgcg cgcgcgaggg
     2761 tccacaggcc ctcgaagttg cgtgacgcgg cgacggacat cgcgggggca gtgacggcca
     2821 gcaggacaac gatcgcgtcg atgtctcccg tcggtggatc ggtgatcgcg tgcaggctca
     2881 cgctggcgtg catgcgcgcg agtgcgcggg tgagcgtatg cgcgtcgtcg gggtcggcgg
     2941 cgagccagac ggtcttgtgg aggggttcgc tggcggcgag cggacgcggg ggccatgcga
     3001 tcgcccgcag caggctggcg ggtggaggcg tgcgtgtggg ctcggggggg atcgtgggcg
     3061 aggtcggggc ggcgtcgatc cagca
//
LOCUS       DASWNJ010000872         2692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_508856, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000872 DASWNJ010000000
VERSION     DASWNJ010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508856"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2692
                     /locus_tag="VGB85_14810"
     CDS             <1..>2692
                     /locus_tag="VGB85_14810"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEY0135352.1"
                     /translation="MPETEPFVATQSFVGKLQGEALTWRRFVHEQAPRGAFINLADLL
                     DPPEQAVGYATVWVKVDRPGPAALHLGTSGAYKVWLDGQLVGEGDTYRTPTPLQSAHP
                     LTLRAGWNRLLIKLATDDAMWGFYARLSAPNGAPLPGLSATIEPPPAAASAAAPAASG
                     AKALAAGTGPKGQGAKASPAAAVKPVGKPPSTTPVRTLRAALEARGTGPRARPADRLH
                     LAEFYRWTVPFAREDRTAVDRAREADTALGSARSAYMLATLDPDQNTSRAALVTAVER
                     ARAEGPSGAPLLAALLVELSLRDRFLGLEWRARALLDEAFRTAPDDMVVELALAERLA
                     EDGFPLAALAWVEDMQKRYPDSAILARQQAERRMNLGRTEEALKILAGLQGAQPTDAS
                     LAAEMQEGLLRLGKAEEAVAIARARVKLVPGLPDTHRALAQLEEARGDLPAARAAMLR
                     ASTLAPQDAELHAQIGRLAARAGDVPAAIQSVRRSLELRPQQPELRDFLATLDTGARD
                     DLLARYPVDLEALAKIETPAAWQGKDAGILLARTAVRVLPNGLSERLDHRVIRVLDDR
                     GIRTQSVQGVVYDPEESYVEVRRARVRRAEGGVEEMGDASVIALTDAGYRMFYDQRQQ
                     RVTFNGLRVGDTLEVAFVRRDTAARNKFDDYFGEIAPLEDTLPQRRREYILEAPAGRK
                     LVFNQPVEATPGKQPGTTLYKLTVGERKGIRAENNMPGWTEIARYLHVSTFSDWDAVG
                     RWYWNLVKEQLVVDAKIRAAVNEVLAGLPKGADERAKVRALYKHVIVSTRYVGLEFGI
                     HGFKPYRTTDVYDRRFGDCKDKASLLKVMLGEAGIKSHLVLVRTRDQGTIGATPASLS
                     AFNHAITYVPSLDLYLDGTAEFSGPEELPTGDQGATVLIVR"
BASE COUNT          393 a         1039 c          932 g          328 t
ORIGIN      
        1 catgccggag acggagccgt ttgtcgccac gcagagcttc gtcggcaagc tgcaggggga
       61 ggcgctgacg tggcgccgct tcgtgcacga gcaggccccg cgcggggcct tcatcaacct
      121 cgccgacctg ctcgacccgc ccgagcaggc ggtcggctac gcgacggtgt gggtcaaggt
      181 cgaccgcccc ggccccgccg cgctgcacct cggcaccagc ggcgcctaca aggtctggct
      241 cgacggtcag ctcgtcggcg agggcgacac ctaccgcacg ccgacgccgc tgcagtccgc
      301 ccacccgctg accctgcgcg ccggctggaa ccgcctatta attaaactcg ccaccgatga
      361 cgcgatgtgg ggcttctacg cccgcctcag cgcccccaac ggcgcgccgc tgccgggcct
      421 gagcgcgacg atcgagccac ctcctgctgc ggcttcggct gcggctccgg ctgcgtccgg
      481 cgcgaaggcc ctggccgcgg ggacaggtcc caaaggacag ggcgcaaagg ccagcccggc
      541 cgccgcggtc aagcccgtcg gtaagccacc ctcgaccacg ccggtgcgca cgctgcgggc
      601 cgcgctcgag gcccgcggca ccggcccgcg cgcgcggccg gccgaccgcc tccacctcgc
      661 cgagttctac cgctggaccg tgcccttcgc ccgcgaggac cgcacggcgg tcgaccgcgc
      721 gcgcgaggcc gacacggcgc tcgggtcggc ccgcagcgcg tacatgctcg ccaccctcga
      781 ccccgaccag aacaccagcc gcgccgcgct ggtgaccgcg gtcgagcggg cgcgggccga
      841 gggcccgtcg ggtgcgccgc tgctggccgc gttgctggtc gagctgtcgc tgcgcgaccg
      901 cttcctcggg ctcgagtggc gcgcgcgggc gctgctcgac gaggcgtttc gcaccgcccc
      961 ggacgacatg gtcgtcgagc tcgcgctcgc cgagcgcctc gccgaggacg gcttcccgct
     1021 cgccgcgctc gcgtgggtcg aggacatgca aaagcgctac cccgactctg cgatcctggc
     1081 ccgccagcag gccgagcgca ggatgaacct cggccgcacc gaggaggcgc tcaagatcct
     1141 cgccggactc cagggcgcgc agccgaccga cgcgagcctc gccgccgaga tgcaggaggg
     1201 cctgctgcgc ctcggcaagg ccgaggaggc ggtggcgatc gcccgcgcgc gcgtcaagct
     1261 cgtgcctgga ctgccggaca cccaccgcgc gctcgcgcag ctcgaggagg cccgcggcga
     1321 cctgccagcg gcccgcgcgg cgatgctgcg cgccagcacg ctggcccccc aggacgccga
     1381 gctgcacgcg cagatcggcc gcctcgccgc gcgcgcgggc gacgtgccgg ccgcgatcca
     1441 gagcgtgcgc cggtcgctcg agctgcggcc gcagcagccc gagctgcgcg acttcctggc
     1501 gaccctcgac accggcgcgc gcgacgacct gctcgcgcgt tacccggtcg acctcgaggc
     1561 gctggcgaag atcgagacgc ccgccgcgtg gcagggcaag gacgccggga tcctgctcgc
     1621 gcgcacggcc gtgcgcgtgc tgcccaacgg cctcagcgag cgcctcgacc accgcgtgat
     1681 ccgggtgctc gacgatcgcg ggatccgcac gcagtcggtc cagggcgtcg tctacgaccc
     1741 cgaggagagc tacgtcgagg tccgccgcgc ccgcgtgcgc cgggccgagg gcggcgtcga
     1801 ggagatgggc gacgccagcg tgatcgccct gaccgacgcc ggctaccgca tgttctacga
     1861 ccagcgccag cagcgggtca cgttcaacgg gctacgcgtc ggcgacacgc tcgaggtcgc
     1921 ctttgtgcgt cgtgacaccg ccgcgcgcaa caagttcgac gactacttcg gcgagatcgc
     1981 gccgctggag gacacgctgc cgcagcgccg tcgcgagtac atcctcgagg cgcccgccgg
     2041 tcgcaagctg gtgttcaacc aacccgtcga ggcgaccccc ggcaagcagc ccggcacgac
     2101 gctctacaag ctgaccgtcg gcgagcgcaa aggcatccgc gccgagaaca acatgcccgg
     2161 ctggacggag atcgccaggt atctccacgt cagcaccttc tccgactggg acgcggtcgg
     2221 ccgctggtac tggaacctcg tcaaggagca gctggtcgtc gacgccaaga tccgcgcggc
     2281 cgtcaacgag gtgctcgcgg ggctccccaa gggcgccgac gagcgggcca aggtgcgcgc
     2341 gctgtacaag cacgtcatcg tgtcgacccg ctacgtcggc ctcgagttcg gcatccacgg
     2401 cttcaagccc taccgcacga cggacgtgta cgaccgccgc ttcggcgact gcaaggacaa
     2461 ggcgagcctg ctaaaggtga tgctcggcga ggccggcatc aagtcgcacc tcgtgctcgt
     2521 gcgcacccgc gatcagggca cgatcggcgc gaccccggcg agcctgtccg cgttcaacca
     2581 cgcgatcacc tacgtgccga gcctcgacct ctacctcgac ggcaccgccg agttctcggg
     2641 ccccgaggag ctgccgaccg gcgatcaggg cgcgacggtc ctcatcgtgc gc
//
LOCUS       DASWNJ010000873         1867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_193252, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000873 DASWNJ010000000
VERSION     DASWNJ010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193252"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1543)
                     /locus_tag="VGB85_14815"
     CDS             complement(<1..1543)
                     /locus_tag="VGB85_14815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEY0135353.1"
                     /translation="MTPAPSSRPPLAVAWTLCLLAAPVAAQGVTRLLSAWTGESHVAP
                     AVTFVALVPVLLAGIVALLTAVARPVVGGSLAALTFAASLLWIGPVAAITTTLIAGWL
                     AAVGLPRLATVVPVPRGRVAGAFWALLAVLAVVQNARMSVFMADPTQRWGALLPSVEF
                     ATKHSCLTSYVHGAALVEHDVNIYDDRYSFESEPQLPAVIDIGPLTMDSYEYPPQFLP
                     LPALFRALTGDFMAIRALWFLLTAAVFAGVSVVIARWLGGEAGRRALLLAPAVWLSPP
                     LLITAYFGNFQSIAMALSALAMLAIFRGHTRSGAALLAFLIGAKIFPGILGVYLLAAR
                     RFAAAAWTAAFSALFAALALLVVGTLPFVDFIKFHLPRLASGETFSFLSEPGATMTNF
                     GAFGIPFKLRLIGWAGSEADAWALARPVAWTYTLIVVAVAVAAGWRRQPATSTQQQVA
                     LLGGWFGLLALGAMRSPFAPPEALIPILWALSLRAAAASRPRDASIAAALWIVMMIVI
                     PTPAPA"
BASE COUNT          331 a          613 c          733 g          190 t
ORIGIN      
        1 ccgccggggc gggggtcggg atcacgatca tcatgacgat ccacagcgcc gcggcgatgc
       61 tggcgtcgcg ggggcgcgag gcggcggctg cccgaaggga cagggcccag aggatcggga
      121 tcagggcctc gggcggggcg aaggggctgc gcatggcccc gagggcgagc aggccgaacc
      181 atccgccgag cagcgcgacc tgctgctgcg tgctcgtggc gggctgtctg cgccacccgg
      241 cggcgacggc gacggcgacg acgatcagcg tgtaggtcca cgcgaccggc cgcgcgagcg
      301 cccaggcgtc ggcctcggag cccgcccagc cgatcaggcg cagcttgaaa gggatgccga
      361 aggcgccgaa gttcgtcatc gtcgcgccgg gctcgctgag gaacgagaag gtctcgccgc
      421 tggcgaggcg cggcaggtgg aacttaataa agtcgacgaa ggggagggtg ccgacgacga
      481 gcagcgcgag cgcggcgaac agggccgaga aggcggccgt ccaggcggcg gcggcgaagc
      541 gacgggcggc gagcagatag acgccgagga tccccgggaa gatcttggcg ccgatcagga
      601 aggcgagcag ggcggcgccg ctgcgggtgt gaccgcgaaa gatcgccagc atcgccagcg
      661 ccgacagggc catggcgatc gactggaagt tgccgaagta cgcggtgatc agcagcggcg
      721 gcgacagcca cacggcaggc gcgagcagca gcgcccgccg cccggcctcg ccgccgagcc
      781 agcgcgcgat cacgacgctg acgcccgcga acacggcggc cgtcagcaag aaccatagcg
      841 cccggatcgc catgaagtcg ccggtgagcg cccggaacag ggccggcagc gggaggaact
      901 gcggcgggta ctcgtaggag tccatcgtca gcgggccgat gtcgatcacc gcgggcagct
      961 gcggctcgga ctcgaagctg tagcggtcgt cgtaaatgtt gacgtcgtgc tcgacgagcg
     1021 cggcgccgtg cacgtagctg gtcaggcacg agtgcttcgt cgcaaactcg accgacggca
     1081 gcagcgcgcc ccagcgctgg gtcgggtcgg ccatgaagac gctcatgcgc gcgttctgga
     1141 cgaccgcgag cacggcgagc agcgcccaga acgctccggc gacgcgcccg cggggcaccg
     1201 gtacgacagt ggccaatctc gggagtccga ctgccgcgag ccagccggcg atcagcgtgg
     1261 tggtgatcgc ggcgaccggc ccgatccaca gcagcgaggc cgcaaaggtc agggccgcga
     1321 gcgacccgcc gacgaccggg cgcgcgacgg cggtcagcag cgcgacgatg ccggcgagca
     1381 gcacaggcac gagcgccacg aaggtgacgg ccggtgcgac gtgcgactca cctgtccacg
     1441 cggacagcag gcgcgtgacg ccctgcgcgg cgaccggagc ggccagcagg cacagcgtcc
     1501 aggcgaccgc gagcggagga cgcgaggagg gagcgggcgt catgggccgg agcttacacg
     1561 cgccacggac agctcgcgcg gcgagttgca gcagcggtgg cggccgttac gatccggccg
     1621 agacgcatgg cggggatcca cacctccggc cggtgccggg gatgagtgcc gatacgacga
     1681 gctttgtgtc gggccgttaa cgcaggcccg cgaacagctc cgcggcccag tcgatgaaca
     1741 cgcgcagcct ggtgctcagg tgccgactcg gcggatagac caggtagacc ggctgacgcg
     1801 cgcgcggcca gtcggtgagc accggcacca gcgcgccgcg ctcgacgtgc ggtcggacga
     1861 tgaagtc
//
LOCUS       DASWNJ010000874         3120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_157418, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000874 DASWNJ010000000
VERSION     DASWNJ010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157418"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VGB85_14820"
     CDS             <1..387
                     /locus_tag="VGB85_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135354.1"
                     /translation="AAPPPAEAGDAPRTRKAKPETKGKSTAKKTDNEPPSSPRPAPQA
                     TAPAAGRAAGYAPKRAEAQASDDKDTGAGRCAELCDLAHATCELEAKICALAQRHTED
                     PHYGEECSRASEDCDLASEACNQCSG"
     gene            complement(451..2802)
                     /locus_tag="VGB85_14825"
     CDS             complement(451..2802)
                     /locus_tag="VGB85_14825"
                     /inference="COORDINATES: protein motif:HMM:NF005901.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rv1355c family protein"
                     /protein_id="HEY0135355.1"
                     /translation="MNDEKMPDARTQHKTLLDELTQLCVTHHHGSEMWRPRLFRAASA
                     EEAEELADLLRSCPEVAVYDTIGPQLKELVETRSPSRALAAKEIRSRIEAVLAGRTPM
                     EYGVWVYYPWSRRLVHILDRPEFIELRTNRNQYKITAEEQRILSTKKVGIVGLSVGQS
                     VALTMAIERSMGEIRLADFDTLGLSNLNRLRAGLHNIEIPKVYITAREIAEIDPFIVV
                     KCYPDGVTMDTIDDFLLSGGPLDLLVDECDSLDIKVWLRHHARHHKIPVLMETSDRGL
                     VDVERFDLEPTRPLFHGLVGDLHPEQLRGLSTSDKIPYVLQIIGEPTISDRARASMLE
                     VGNSIKTWPQLASAVTLGGGAAADVARRMNLGLIRHSGRYWVDVETVVPNTVDARPAP
                     TPAPTLPPSLSAGHMLELAGLVPADGPAPLHIAPELREKLLAAAVAAPSGGNSQPWRW
                     LAHGDRLHLFHDRTRSSALPDYHGHGGLVALGAAIENFVLAAHAERLEVVLRTFPLGP
                     SHDLVASFTLHDRPVPGAEPHTHDELAPMISVRHTNRKSARERLPADAIADMTAAVRS
                     IAGAELQLLEDDGELASIGALVGTGDRLRFLDQACNHDLLSEVRWSAEEAGQRRDGLS
                     FDDLELSRGDRAVMQMLRSWPSLQLIRAWGGGRGLDKPSFQAIISSAAVGLVTMPRMG
                     DAEYLLGGRAVERLWLTAQARGLAFHPMTALPYMFARVFHGDGVGLEPGTVAALRRLR
                     EPYRRLLSVTEDTAEVMLFRVSRAEPTEARALRRPLADVLVVA"
     gene            complement(2795..>3120)
                     /locus_tag="VGB85_14830"
     CDS             complement(2795..>3120)
                     /locus_tag="VGB85_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135356.1"
                     /translation="AIARPAIARPAIAGASGNMHYQRMLHAPAFIGSTSPSRRGAPRR
                     SISLDSRQFHRGPVKRSPARGRGRQRGVTAVNLLAGRRKRRWSSFPVSDMVAAGRVMD
                     LRRSE"
BASE COUNT          464 a         1057 c         1152 g          447 t
ORIGIN      
        1 gccgcgccgc cgccggcaga ggccggcgac gccccgcgca cgcgcaaggc gaagcccgag
       61 accaagggca agtcgaccgc gaagaagacg gacaacgaac ccccttcgag cccgcgaccc
      121 gccccccagg cgacggcgcc cgcggccggt cgcgctgccg gctacgcccc caagcgggcc
      181 gaggcccagg cgagcgacga caaggatacc ggcgcgggcc gctgcgccga gctctgcgac
      241 ctcgcccacg cgacctgcga gctcgaggcg aagatctgcg cgctcgcgca gcggcacacc
      301 gaggacccgc actatggcga ggagtgcagc cgcgccagcg aggattgcga cctcgcctcc
      361 gaggcgtgta atcaatgctc gggttgactc cacggccctc cgggcctgag gcgacttcac
      421 gcccctccgg gcctgagcct gacctttcgg tcaggcgacg acgagcacgt cggcgagcgg
      481 ccgccgcagc gcgcgtgcct cggtcggctc ggcccgggac acgcggaaca gcatgacctc
      541 ggcggtgtcc tcggtgacgc tgagcagccg gcgatagggc tcgcgcagtc ggcgtagcgc
      601 ggcgaccgtg cctggctcga ggccgacgcc gtcgccgtgg aacacgcggg cgaacatata
      661 gggcagcgcg gtcatcgggt ggaacgcgag gccgcgggcc tgggcggtca gccacaggcg
      721 ttcgacggcg cgaccgccga gcaggtactc ggcgtcgccc atgcgcggca tggtgaccag
      781 gccgaccgcc gcgctgctga tgatcgcctg gaagctcggc ttgtcgaggc cacggccgcc
      841 gccccacgcg cggatcagct ggagcgaggg ccacgagcgc agcatctgca tcacggcccg
      901 gtcgccgcgg ctgagctcga ggtcgtcgaa cgacagcccg tcgcgccgct gcccggcctc
      961 ctcggccgac cagcgcacct cggacagcag gtcgtggttg caggcctggt cgaggaagcg
     1021 caggcggtcg ccggtgccga cgagcgcgcc gatgctggcg agctcgccgt cgtcctcgag
     1081 cagctgcagc tccgcgccgg cgatcgaccg caccgcggcg gtcatgtcgg cgatggcgtc
     1141 cgcgggcagc cgctcgcgcg ccgacttgcg attggtgtgg cgcacggaga tcatcggggc
     1201 gagctcgtcg tgcgtgtgcg gctcggcgcc cggcaccggc cggtcgtgca gcgtgaagct
     1261 ggcgacgagg tcgtgcgagg gtccgagcgg gaacgtgcgc agcaccacct cgaggcgctc
     1321 ggcgtgggcg gcgagcacga agttctcgat cgccgcgccg agcgcgacca ggccgccgtg
     1381 gccgtgatag tccggcagcg cggacgagcg ggtccggtcg tggaacaggt gcagccggtc
     1441 gccgtgggcc agccagcgcc acggctggct gttgccgccc gacggcgccg cgacggcggc
     1501 cgccagcaac ttctcgcgca gctccggcgc gatgtgcagt ggggccgggc cgtcggcggg
     1561 caccaggccg gcgagctcga gcatgtgccc ggcggacagc gagggtggca gcgtcggggc
     1621 gggggtcggg gccgggcggg cgtcgacggt gttggggacg acggtctcga cgtcgaccca
     1681 gtagcgaccg gagtggcgga tgaggccgag gttcatgcga cgggcgacgt ccgccgccgc
     1741 gccgccgccg agggtcacgg ccgaggccag ctgcggccag gtcttgatgc tgttgccgac
     1801 ctcgagcatc gacgcgcgcg cgcggtcgga gatcgtcggc tcgccgatga tctggaggac
     1861 gtaggggatc ttgtcgctgg tcgacagccc gcgcagctgc tcggggtgca ggtcgccgac
     1921 gaggccgtgg aacagcgggc gggtcggctc gaggtcgaag cgctcgacgt cgacgaggcc
     1981 gcggtcgctg gtctccatca gcaccgggat cttgtggtgc cgggcgtggt ggcgcagcca
     2041 gaccttgatg tcgaggctgt cgcactcgtc gacgagcagg tcgagcgggc cgcccgagag
     2101 caggaagtcg tcgatcgtgt ccatcgtgac cccgtccggg taacacttga cgacgatgaa
     2161 cgggtcgatc tcggcgatct cccgcgccgt gatgtacacc ttcgggatct cgatgttgtg
     2221 caggcccgcg cgcagtcgat tgaggttgga gaggccgagc gtgtcgaagt ccgcgagccg
     2281 gatctcgccc atgctgcgct cgatcgccat cgtcaacgcc accgattgac cgaccgaaag
     2341 tccgacgatt ccgacctttt ttgtggagag gatccgctgc tcctccgcgg tgatcttgta
     2401 ctgattacga ttggtgcgaa gctcgatgaa ttccgggcga tcgaggatat gaaccagccg
     2461 gcgcgaccac gggtaatata cccatacgcc atattccatc ggcgtgcgcc cggcgagcac
     2521 cgcctcgatc cggctgcgga tctccttggc ggccagcgct cgggacgggc tgcgcgtctc
     2581 gacaagctcc ttgagctgcg ggccaattgt gtcgtaaacg gccacttcgg ggcagctgcg
     2641 cagcaggtcg gcgagctcct ccgcctcctc cgcggacgcg gcgcggaaca gccgcgggcg
     2701 ccacatctcg ctgccgtgat gatgggtgac gcataattgc gtcagctcgt cgagcagcgt
     2761 cttatgctgt gtccgcgcgt ccggcatctt ttcgtcattc actccggcgc aggtccatga
     2821 ccctaccggc ggcgaccata tcgctaaccg gaaaggaact ccagcggcgt ttgcggcgcc
     2881 cggcgagcag attgaccgca gtcacgccgc gctgccggcc gcggccgcgc gcgggcgagc
     2941 gtttcaccgg gcctcgatga aattgccgcg aatcgagtga aatcgagcgt cgaggcgcgc
     3001 cgcgccgcga cggggacgtc gacccgataa atgccggtgc atgcagcatt ctttggtagt
     3061 gcatgttgcc gctggctcct gcgatcgcag gtcgtgcgat cgcaggtcgt gcgatcgcgg
//
LOCUS       DASWNJ010000875         2204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_327609, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000875 DASWNJ010000000
VERSION     DASWNJ010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327609"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2204)
                     /locus_tag="VGB85_14835"
     CDS             complement(<1..>2204)
                     /locus_tag="VGB85_14835"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0135357.1"
                     /translation="PAPQRPSSIPHLDQLLESPSLSGGDEWPKPEREMLPPGHKVGRF
                     TLLQRLGVGAMGVVYAAYDPELDRRIAVKLLRTREGRGAARAQARLLREAQAMARLSH
                     PNVAVVHDVGTHEGDVFVAMEFIRGDTLQAWLRKSPRKWSEIVDVFVEAGRGLAAAHA
                     AGLVHRDFKPTNAMIGDDGRVRVLDFGLCFAETTVDTESTGDMHVDVRITRREEVVGT
                     PAYMPPEQFLRGGVVGPASDQFSFCASLYEALHDQLPFPGETVHEVSLAIAAGELRPP
                     PRQSRVPPWVNNVIQRGLKPNAADRFPSMEALLRVLGRNRTRTRGSLVIAGTLALATG
                     FVGFWAARSQAVAVDPCSGGAAEVAEVWGVTPRAAAESALVSAGPAYAEEIWPRVASD
                     LDQYADEWQGLHREACLAHQRGETSGVLLDRRMACLARRKAALGEAVKVLSDANAEVA
                     LHALEVVNNLPALDRCNDLEALAAEVPPPSDPQVRARFDALRPRLARVRTLDHAGLTA
                     MALGEADDVLRAAEALAEPTLLSEALLQRGRLGIHSRDQPAEKEALLTRAYLTALASR
                     LDEFAAEALALRLYVRGRDGSKAAKALDDLPVAEAMVARLPAPGRLRGLLLNNAGSVS
                     WALGDAQRGAALFREALAVREAALGRDHLDVAFTLVNLAMVTPAEDQERMRLMQRALT
                     IFDDQLGRAHPQTIDVRMTASLYALDPREAQTLLAPGCEALGRFSPDDRAQR"
BASE COUNT          277 a          809 c          848 g          270 t
ORIGIN      
        1 cgcgctgggc ccggtcgtcc ggcgagaacc gaccgagcgc ctcgcagccg ggcgcgagca
       61 gggtctgggc ctcgcgcggg tcgagggcgt acaggctggc cgtcatgcgc acgtcgatcg
      121 tctgcggatg agcccggccg agctggtcgt cgaagatcgt cagggcccgc tgcatcaggc
      181 gcatgcgctc ttggtcctcc gccggcgtga ccatcgccag gttgaccagc gtgaacgcga
      241 cgtcgaggtg gtcccggccg agcgccgcct cgcggaccgc cagcgcctcg cggaacagcg
      301 ccgccccgcg ctgcgcgtcg cccagcgccc agctcaccga gccggcgttg ttgagcagca
      361 gcccgcgcag gcggccgggg gccggcaggc gggcgaccat cgcctcggcg acgggcaggt
      421 cgtcgagcgc cttggcggcc ttggagccgt cgcgcccgcg gacgtacagg cgcagcgcga
      481 gggcctcggc ggcgaactcg tcgaggcgcg aggcgagcgc ggtcaggtag gcgcgcgtca
      541 gcagggcctc cttctcggcc ggctgatcgc ggctgtgaat tcccaggcgg ccgcgctgca
      601 gcagcgcctc gctgagcagc gtcggctcgg cgagcgcctc ggcggcgcgc agcacgtcgt
      661 cggcctcgcc gagcgccatc gccgtcagac ccgcgtggtc gagcgtgcgc acgcgggcga
      721 gccgcggacg cagcgcgtcg aagcgcgcgc gcacctgcgg gtccgagggc ggcgggacct
      781 cggcggcgag cgcctcgagg tcgttgcagc ggtcgagcgc cggcaagttg ttgacgacct
      841 cgagcgcgtg cagggcgacc tcggcgttgg cgtccgagag caccttgacg gcctcgccga
      901 gcgcggcctt gcggcgcgcg aggcaggcca tgcggcggtc gagcagcacg ccggaggtct
      961 cgccgcgctg gtgtgccagg caggcctcgc ggtgcaggcc ctgccactcg tcggcgtact
     1021 ggtcgaggtc gctggcgacg cgcggccaga tctcctcggc gtaggcggga ccggcggaca
     1081 ccagcgccga ctcggccgcg gccctcggcg tcacgcccca cacctcggcg acctcggccg
     1141 cgccgccgga gcacgggtcg accgcgacgg cctggctgcg cgccgcccag aagccgacga
     1201 agccggtcgc cagcgccagc gtcccggcga tcaccaggct gccgcgcgtg cgcgtgcggt
     1261 tgcgtccgag cacccgcagc agcgcctcca tcgacgggaa gcggtcggcc gcgttcggct
     1321 tgaggccgcg ctggatcacg ttgttgaccc acgggggcac ccggctctgc cgcggcggcg
     1381 ggcgcagctc gccggccgcg atcgccaggc tgacctcgtg caccgtctcg ccggggaacg
     1441 gcagctggtc gtggagcgcc tcgtagagcg acgcgcagaa gctgaactgg tccgaggccg
     1501 ggccgaccac gccgccgcgc aggaactgct ccggcggcat gtaggccggc gtgccgacga
     1561 cctcctcgcg ccgcgtgatg cggacgtcga cgtgcatgtc gccggtcgac tcggtgtcga
     1621 cggtggtctc ggcgaagcac aggccgaagt cgaggacgcg cacgcgaccg tcgtcgccga
     1681 tcatcgcgtt ggtcggcttg aagtcgcggt ggaccaggcc cgccgcgtgc gcggccgcca
     1741 gcccgcggcc cgcctcgacg aacacgtcga cgatctccga ccacttgcgc ggcgacttgc
     1801 gcagccacgc ctgcagcgtg tcgccgcgga tgaactccat cgccacgaag acgtcgccct
     1861 cgtgggtgcc gacgtcgtgc acgacggcga cgttggggtg cgacaggcgg gccatcgcct
     1921 gggcctcgcg cagcagtcga gcctgggccc gcgcggcgcc gcggccctcg cgggtgcgca
     1981 gcagcttgac ggcgatcctg cggtcgagct cggggtcgta ggcggcgtag acgaccccca
     2041 tcgcgccgac gccgagccgc tgcagcaggg tgaagcggcc gaccttgtgc cccggcggca
     2101 acatctcgcg ctcgggcttg ggccactcgt cgccgcccga cagcgacggc gactcgagca
     2161 gctggtcgag atgagggatc gacgacggcc gctgcggcgc cggg
//
LOCUS       DASWNJ010000876         2398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_546999, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000876 DASWNJ010000000
VERSION     DASWNJ010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546999"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2398)
                     /locus_tag="VGB85_14840"
     CDS             complement(<1..>2398)
                     /locus_tag="VGB85_14840"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0135358.1"
                     /translation="LIEYGWRTEQQGGDLTLALTSYRSALAEVPDHPVASALAIECAL
                     RSGSRDVAEPLLARQLEILDIPGLRAADLLDLADLAETPQRRREQLLLAIANDPHETT
                     ALRRLIRVLRAEGADAPPILELPGLYRRLAALAADHVTAASALQLAVLTSGTAEVPRD
                     VLEDIRTWLAAHGKDPAAHGILDALVSNFAQRDPRGTQEHGVEGLRGVDVLVRLAAYK
                     DDPREQAILREQIARLRWEASPLASRQSALDDDGTLRRLAEEDAAPPALPDAERDAYR
                     VLESDLRFVRRYLPEQRWVWQALASVLRVLGDTTALIAHLTEWAQTRSHGTERATILL
                     RLGFVHERLEHDMSRAAEVYELAVAEDPKNTACLRALGSVYERMRRWAQAAAILQRQA
                     RETTDGPERLAALRRVAAIAEQELHDVDLAIGTLQEIAQIDQDDVLSLFQLAALCRTH
                     DRANILIQTLQLLVERLDEDIARTAILVELGEAHETRLKQRTAARESYERALKLSPGY
                     TPALRALARLYRDGGDLEALLRLHEPDVDATSDPAVLALKAGRICFEELGDMERAIGY
                     LWRSYRINPDLVPAREILLQLLSATGKIRDAYDLLRAQDPPHSPALLADYHYRLGLIA
                     EALARQGHDNPLANALADEDRALQHYRAALAVQHDHGLAFERSRRLLVANNDLPNLIR
                     LLEDQLQHASGSNRAALLVQLARLHLGTGDFERARGSYEIAIEAAPDDGVILREYKTL
                     LRKLGDTTTLPTLRLRLARTSEDTHYKATLLVESAEVLLQSEAPEDREFAAGAILGAL
                     R"
BASE COUNT          303 a          774 c          979 g          342 t
ORIGIN      
        1 cgcaacgcgc cgaggatcgc accggccgcg aactcgcggt cctcgggggc ctcggactgc
       61 aacaggacct cggcggactc gaccaacaac gtcgccttgt agtgcgtgtc ctcggaggtc
      121 cgggccaggc gcaggcgcag ggtcggcagg gtggtggtgt cgccgagctt gcgcagcagg
      181 gtcttgtact cgcgcaggat cacgccgtcg tcgggggcgg cctcgatcgc gatctcgtag
      241 ctgccgcgcg cgcgctcgaa gtcacctgtc ccgaggtgca ggcgagcgag ctggaccagc
      301 agcgcggccc tgttggagcc gctggcgtgc tgcagctgat cctcgagcag gcggatcaaa
      361 ttgggcaggt cgttgttggc gacgagcagc cggcggctgc gctcgaaggc gaggccgtgg
      421 tcgtgctgga ccgccagcgc ggcgcgatag tgctggagcg cgcggtcctc gtcggcgagc
      481 gcgttggcca gcgggttgtc gtggccctgg cgcgcgagcg cctcggcgat caggccgagc
      541 cggtagtgat aatcggcgag cagcgccggg ctgtgcggcg ggtcctgggc gcgcaggaga
      601 tcgtaggcgt cgcggatctt gccggtcgcg ctcaggagct ggagcaagat ctcgcgcgcc
      661 ggcacgaggt ccgggttgat gcggtacgat cgccacaggt agccgatcgc gcgctccatg
      721 tcgccgagct cctcgaagca gatccggccg gccttgagcg cgagcacggc ggggtcgctg
      781 gtcgcgtcga cgtcgggctc gtgcaggcgc agcagcgcct cgaggtcgcc gccgtcgcgg
      841 tacaggcggg cgagggcccg cagcgcgggg gtgtagccgg ggctgagctt gagcgcgcgc
      901 tcgtacgact cccgggccgc agtacgctgc ttgaggcggg tctcgtgggc ctcgccgagc
      961 tcgacgagga tcgcggtgcg ggcgatgtcc tcgtcgaggc gctcgacgag cagctgcagg
     1021 gtctggatca ggatgttcgc gcggtcgtgc gtgcgacaca gggccgcgag ctgaaacagc
     1081 gacaggacgt cgtcctgatc gatctgggcg atctcctgca gcgtgccgat cgccaggtcg
     1141 acatcgtgga gctcctgctc ggcgatcgcg gcgacgcggc gcagggcggc gaggcgctcg
     1201 gggccgtcgg tggtctcgcg ggcctgacgc tgcaagatcg cggcggcctg ggcccagcga
     1261 cgcatgcgct cgtagacgct gccgagcgcg cgcaggcagg cggtgttctt cgggtcctcg
     1321 gcgacggcga gctcgtagac ctccgcagca cgtgacatgt cgtgctcgag gcgctcgtgc
     1381 acgaagccga ggcgcagcag gatcgtcgcg cgctcggtgc cgtgcgagcg ggtctgggcc
     1441 cactcggtga ggtgggcgat caacgcggtc gtgtcaccga gcacgcgcag cacgctcgcc
     1501 agcgcctgcc acacccagcg ctgctcgggc aggtagcggc ggacgaaccg caggtcggac
     1561 tcgaggacgc ggtaggcgtc gcgctcggcg tcggggagcg cgggcggggc ggcgtcctcc
     1621 tcggcgagcc ggcgcagggt gccgtcgtcg tcgagggcgc tctggcgcga ggccagcggc
     1681 gaggcctccc agcgcaggcg ggcgatctgc tcgcgcagga tcgcctgctc gcgcgggtcg
     1741 tccttgtagg cggccaggcg gacgagcacg tcgacgccgc gcagaccctc gacgccgtgc
     1801 tcctgcgtgc ctcgtgggtc acgctgggcg aagttgctga ccagcgcgtc gaggatgccg
     1861 tgcgcggccg ggtccttgcc gtgcgccgcg agccaggtgc ggatgtcctc gaggacatct
     1921 cgcgggacct cggcggtgcc ggaggtcagc acggcgagct ggagggcgct cgcggcggtg
     1981 acgtggtcgg cggcgagggc cgcgaggcgg cggtacaggc cgggcagctc gaggatcggc
     2041 ggggcgtcgg cgccctcggc ccgcagcacg cggatcaggc ggcgcagggc ggtggtctcg
     2101 tgcgggtcgt tggcgatcgc cagcaacagc tgttcgcggc ggcgctgcgg ggtctcggcg
     2161 aggtcggcga ggtcgagcag gtccgcggcg cgcaggccgg ggatgtcgag gatctcgagc
     2221 tggcgcgcga gcaagggctc ggcgacatcc cgtgaaccac tgcgcagcgc gcactcgatc
     2281 gccagcgcgc tggcgaccgg gtggtcgggg acctcggcga gggcgctgcg gtagctggtc
     2341 agcgcgagcg tcaggtcgcc gccctgctgc tcggtgcgcc agccgtactc gatcagcg
//
LOCUS       DASWNJ010000877         3630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_547012, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000877 DASWNJ010000000
VERSION     DASWNJ010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547012"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2065
                     /locus_tag="VGB85_14845"
     CDS             <1..2065
                     /locus_tag="VGB85_14845"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0135359.1"
                     /translation="RREDIVALLNTTPRAGRRLHAPVIIIPPAHGKRKESTSIFAQIL
                     VESFARARRDVVVLRFDGIRNIGESYKDPDCREPGREAMRSSLSQAVDDIHTVVEYAH
                     NNPLFSPSELVLCCFSLQGVPGRRATFLDQGKRINYLIGVNGAVAAQEMIRNAAGGVD
                     YIAQRASGTHIGTAAILGINIDGDPYADDLIRNGLAFMSDAKREIAAIPIPVTWFLGR
                     HDAWIDPATIREFIGVPANAPRELIELDIGHLPLTSFEALTLFSELVKCIGRQVLHED
                     LEPAIPDPREMTRVRHAEWRRVPKSPLPDRRSYWHTYLLGDTEHKVGYDVLNAADEYL
                     GFLDREAELLDLRPEHHAADLGCGTGNFAARLLRRRGHKTRPVCARLALVDFVSDALD
                     EAERKLHVLARERELAIPTVTRHLVNLDIHPIRTLRRFVAGELQGYDALKGILRGLSD
                     YSLDTWRAHEDWRLHDIVRGRELDKQDLAYVRANFPADEQEVILDTNRLTRWLRGRWT
                     VEDMTREGRARVDRGEPLSAGDLRLSRLSFQTGDMVERLPFADASLDRISCSLVLSYL
                     NNPLELLREFYRVLRPGGRLVVSSMLPDFDMSRIYQRVLRRIETDPNLPLPPGMDRPT
                     FASEMRAFLNHAAFLTILTEEGQFSFFSRDEMCSLVERAGFRRVENHLAFGEPPQAHI
                     TVGNR"
     gene            2092..>3630
                     /locus_tag="VGB85_14850"
     CDS             2092..>3630
                     /locus_tag="VGB85_14850"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0135360.1"
                     /translation="MSSTSFRRDDAFALQLRGELRAGYQRATWLSVALILGFVILDAT
                     TVREQGLLYTFFALRAGICILLVGLLRLSEHPRLAPHVELIAMIAASATGGVIALMSA
                     LYMGHQSYYYAGMSLTLVAIGVLVPVGPLPIALTAAAIYLSFVLFLQLLDPVPLSEWN
                     WNVFVANSIFLIVTAAVVVIGAFINRRLRRRAFDAAWEQERASEELRLVNERVKQSYL
                     EISEKNREIEDAYRSKSQFLDNISHELRTPLTCILTPLEGLLAGNARGEIRGVFEDMH
                     SASRQLYDLINDLLDYSKYGSRDQPMQRDPIDLRAVVDEHVRSFKPTARQRMLTLEWD
                     APAEPLIARGSSRELGKVVRNLLSNAVKFTPFGGRVRVLLTGDGPTAKLSVEDSGIGM
                     SPEVQSKLFTPFFQGDATSTRAFEGTGIGLALVKTIVERHEGDIRVHSVAGQGTTMTV
                     SLPLSPLDPTELMGMELGPLSSGASIIAPVPQFSEPSQKILVTEELLQSEIDNMRARS
                     GGGTG"
BASE COUNT          566 a         1427 c         1098 g          539 t
ORIGIN      
        1 gcggcgcgag gacatcgtcg ccctgctcaa caccaccccg cgcgccggcc ggcgcctgca
       61 cgcgccggtg atcatcatcc cgcccgcgca cggcaagcgc aaggagtcga ccagcatctt
      121 cgcgcagatc ctcgtcgaga gcttcgcccg ggcccgccgc gacgtcgtcg tgctgcgctt
      181 tgacggtatt cgcaacatcg gcgagtccta caaggacccc gactgccgcg agcccggccg
      241 cgaggcgatg cgctcgagcc tgtcgcaggc cgtcgacgac atccacaccg tcgtcgagta
      301 cgcgcacaac aaccccctgt tttcgccgag cgagctggtc ctgtgctgct ttagcctcca
      361 gggcgtgccc ggtcgccggg cgaccttcct cgatcagggc aagcggatca attatctgat
      421 cggcgtcaac ggggccgtcg ctgcccagga gatgatccgc aacgccgccg gtggcgtcga
      481 ttacatcgcc cagcgcgcca gcggcacgca catcggcacc gccgcgatcc tcggcatcaa
      541 catcgacggc gacccctacg ccgacgacct gatccgcaac ggcctcgcgt tcatgagcga
      601 cgccaagcga gagatcgccg cgatcccgat ccccgtcaca tggttcctcg gccgccacga
      661 cgcgtggatc gacccggcga cgatccgcga attcatcggc gtgcccgcca acgccccgcg
      721 cgagctgatc gagctcgaca tcggccacct gccgctgacc tccttcgagg cgctgaccct
      781 gttctccgag ctcgtgaagt gcatcggacg ccaggtgttg cacgaggacc tcgagccggc
      841 gatccccgac ccccgcgaga tgacccgcgt ccgccacgcc gagtggcgcc gcgtgcccaa
      901 gagcccgctg cccgaccgcc gctcgtactg gcacacgtac ctgctcggcg acaccgaaca
      961 caaggtcggc tacgacgtgc tcaacgccgc cgacgagtac ctcggcttcc tcgaccgcga
     1021 ggccgagctg ctcgacctgc gccccgagca ccacgccgcc gacctcggct gcggcaccgg
     1081 caacttcgcc gcgcggctgc tccgccgccg cggccacaag acccgcccgg tgtgcgcccg
     1141 cctcgccctg gtcgacttcg tgtccgacgc cctcgacgag gccgagcgca agctgcacgt
     1201 gctcgcgcgc gagcgcgagc tcgcgatccc gaccgtcacg cgccacctcg tcaacctcga
     1261 catccacccg atccgcaccc tgcggcgctt cgtcgccggc gagctgcagg gctacgacgc
     1321 cctcaagggc atcctgcgcg gcctgagcga ctactcgctc gacacctggc gcgcccacga
     1381 ggactggcgc ctccacgaca tcgtccgcgg ccgcgagctc gacaagcagg acctcgccta
     1441 cgtgcgcgcc aacttccccg ccgacgagca ggaggtcatc ctcgacacca accgcctgac
     1501 ccgctggctg cgcggccgct ggaccgtcga ggacatgacc cgcgagggcc gcgcccgcgt
     1561 cgaccgcggc gagccgctca gcgccggcga cctgcgcctc agccgtctgt cctttcagac
     1621 aggtgacatg gtcgagcgcc tgcccttcgc cgacgccagc ctcgaccgga tcagctgcag
     1681 tctggtcctg agctacctca acaacccgct ggagctgctg cgcgagttct accgcgtgct
     1741 gcgcccgggg ggccgtctgg tcgtctcctc gatgctgccc gacttcgaca tgagccggat
     1801 ctatcagcgt gtgctacgcc gcatcgagac cgacccgaac ctgccgctcc cgcccggcat
     1861 ggatcggccg accttcgcca gcgagatgcg cgccttcctc aaccacgccg ccttcctcac
     1921 gatcctcacc gaggagggcc agttctcgtt cttcagccgc gacgagatgt gcagcctcgt
     1981 cgagcgcgcc ggctttcgcc gggtcgagaa ccacctcgcc ttcggtgagc cgccgcaggc
     2041 tcacatcact gtcggaaatc gatgacccga cgcccggacc tcaccgccag catgagcagc
     2101 acgtcctttc gccgggacga cgccttcgcg ctgcagcttc gcggtgagct ccgggccggg
     2161 taccagcgcg cgacctggct cagcgtcgcg ctgatcctcg gctttgtgat cctcgacgcg
     2221 acgaccgtcc gggagcaggg cctgctgtac accttcttcg ccctgcgcgc cggcatctgc
     2281 atcctgctcg tcggtctcct gcgcctcagc gagcacccgc gcctggcccc gcacgtcgag
     2341 ctgatcgcca tgatcgccgc ctccgccacc ggcggcgtca tcgccctgat gagcgcgctc
     2401 tacatgggcc atcagagcta ctactacgcc ggcatgagcc tgacgctggt cgcgatcggc
     2461 gtgctcgtgc cggtcggtcc gctcccgatc gcgctcaccg ccgccgcgat ctacctcagc
     2521 ttcgtcctgt tcctccagct cctcgatccg gtcccgctca gcgagtggaa ctggaacgtc
     2581 ttcgtcgcca acagcatctt cttgatcgtc accgccgcgg tcgtcgtcat cggggcgttc
     2641 atcaaccgcc gcctgcgccg ccgggccttc gacgccgcgt gggagcagga gcgcgccagc
     2701 gaggagctgc gcctcgtcaa cgagcgcgtc aagcagagct acctcgagat ctcggagaag
     2761 aaccgcgaga tcgaggacgc ctaccgcagc aagagccagt tcctcgacaa catctcgcac
     2821 gagctgcgca ccccgctgac ctgcatcctc accccgctcg agggcctgct cgccggcaac
     2881 gcgcgcggcg agatccgcgg cgtgttcgag gacatgcaca gcgcctcgcg ccagctctac
     2941 gacctgatca acgacctgct cgactacagc aagtacggat cgcgcgatca gccgatgcag
     3001 cgcgacccga tcgacctgcg cgccgtcgtc gacgagcacg tgcgctcgtt caagccgacc
     3061 gcgcgccagc gcatgctcac cctcgagtgg gacgcgcccg ccgagccgct gatcgcccgc
     3121 ggcagcagcc gcgagctcgg caaggtcgtc cgcaacctgc tcagcaacgc cgtcaagttc
     3181 acgccctttg gcggccgcgt gcgcgtcctg ctgaccggcg acggcccgac cgccaagctc
     3241 tcggtcgagg actccggcat cggcatgagc cccgaggtcc agagcaagct gttcaccccg
     3301 ttcttccagg gcgacgcgac ctcgacgcgc gccttcgagg gcaccggcat cggcctcgcg
     3361 ctggtcaaga cgatcgtcga gcgccacgag ggtgacatcc gcgtgcacag cgtcgccggc
     3421 cagggcacca cgatgaccgt cagcctgccg ctgagcccgc tcgacccgac cgagctgatg
     3481 ggcatggagc tcggcccgct gtcgagcggc gccagcatca tcgccccggt gccgcagttc
     3541 tcggagccct cgcagaagat cctcgtcacc gaggagctct tgcagagcga gatcgacaac
     3601 atgcgtgccc gcagcggcgg cggcaccggc
//
LOCUS       DASWNJ010000878         1572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_51681, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000878 DASWNJ010000000
VERSION     DASWNJ010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51681"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..614)
                     /locus_tag="VGB85_14855"
     CDS             complement(102..614)
                     /locus_tag="VGB85_14855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0135361.1"
                     /translation="MTRLSIRLARSCDASAMARVHVAAVAHLCRTHYRPHELARWTNQ
                     GPGLYTALVRSATVVVAERNGVVVGFAAVSLAAGVVRAVYVHPGHAGGGVGGRLLARI
                     ERAARVYGVRRLVLDATLNATPFYSRAGYRPIARTRSGLGLKCVRMSKVLWTPRRLNA
                     PGSGGVRGRL"
     gene            717..1427
                     /locus_tag="VGB85_14860"
     CDS             717..1427
                     /locus_tag="VGB85_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135362.1"
                     /translation="MSACVVTLGVLPACDDRQARKDLAEREQQIAQLERKVEELSARP
                     GPPPLPTAPPPAPAVSSLDADIVRAAGSAAGVQKLKLECEADGSVREASLYHMDEAAL
                     PPAVLALREQQYPGSKLRAYETEYEREHGRLFEVEVTTRDGQECEYSAKPDGTLVYTE
                     CHVDAKTLSAGLRAALEHKLPDAEILEAEKKTYADGRVLHSVEVRAGGKVHELYFEDD
                     AVVRHEIVVPAQLEVPAP"
BASE COUNT          244 a          602 c          540 g          186 t
ORIGIN      
        1 gcacgcccgt catagccggc cgcgcacgcc cgtcatagcc ggccgcgcag gcccgtcatc
       61 gccggccgcg caggcccgtc atcgccggcc gcgcaggccc gtcatagccg gccgcgcacg
      121 ccgccggagc cgggcgcgtt caaccttcgc ggcgtccaca gcaccttcga catgcgcacg
      181 cacttgaggc cgaggcccga ccgtgtgcgg gcgatcggtc ggtagccggc gcggctgtag
      241 aatggcgtcg cgttgagggt cgcgtcgagc accaggcggc gcaccccgta cacccgcgcc
      301 gcgcgctcga tgcgggccag caggcggccg ccgacgcccc cgccggcatg cccagggtgc
      361 acatacaccg ctcgcaccac gccggccgcc agcgacaccg cggcgaagcc gacgaccacg
      421 ccgttacgct cggcgacgac gaccgtcgcc gaccgcacca gcgccgtgta gagcccggga
      481 ccctgattgg tccagcgggc cagctcgtgg ggacggtagt gggtgcggca caggtgggcc
      541 acagcggcca cgtgcacccg cgccatcgcg gaagcgtcgc aggagcgggc cagccggatc
      601 gacagcctcg tcacgcccct caggataacc gaagcccgtc gtgcgctcgc cggctttgtg
      661 tatgctggcc ccttcaccga caccccgtgg aggccgcgat gatggttcga gcgatcatga
      721 gtgcgtgcgt cgtgaccctg ggagttttac cggcctgcga cgaccggcag gcccgcaagg
      781 acctcgccga gcgcgagcag cagatcgccc agctcgagcg caaggtcgag gagctgtccg
      841 caaggccagg tccgccgcca ctcccgaccg cgcccccgcc ggcccccgca gtctccagcc
      901 tcgacgccga catcgtgcgc gccgcgggct cggcggccgg tgtgcagaag ctcaagctcg
      961 agtgcgaggc cgacggcagc gtgcgcgagg cctccctcta ccacatggac gaggccgccc
     1021 tgccgcccgc cgtgctggcc ctgcgcgagc agcagtaccc cggcagcaag ctgcgcgcct
     1081 acgagaccga gtacgagcgc gagcacggcc gcctgttcga ggtcgaggtg accaccaggg
     1141 acgggcagga gtgcgaatac agcgcgaagc ccgacggcac cctcgtctac accgagtgcc
     1201 acgtcgacgc gaagaccttg agtgcgggcc tgcgcgccgc gctcgagcac aagctgccgg
     1261 acgccgagat cctcgaggcc gagaagaaga cctacgccga cggtcgcgtc ctgcactcgg
     1321 tcgaggtgcg cgccggcggc aaggtgcacg agctctactt cgaggacgac gccgtcgtcc
     1381 gccacgagat cgtcgtcccg gcccagctcg aggtccccgc gccctgagct gcggacgcgg
     1441 acccgggccc gagcaggccc atatcacgtg gtcacgtggt cacgtggtca cgtggtcacg
     1501 cgcatcgaca cgcggcccgc gactcactcg cgggccggcg cgacctcagt cgtcgccgaa
     1561 gtcgtcgctc tc
//
LOCUS       DASWNJ010000879         3229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_474926, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000879 DASWNJ010000000
VERSION     DASWNJ010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474926"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /locus_tag="VGB85_14865"
     CDS             <1..1324
                     /locus_tag="VGB85_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135363.1"
                     /translation="IDKNGLIEVAVTAEHADGVRMEIDTGEPIELTPTGPGAFGGQIP
                     VFTGLDNGKHSAFLTPWRDTLVGESVAANYVIALPPPGYEVAWQTTDTEGQVVAIDVL
                     PDGRPVELGTYEEMGEPRCYLQLREKNGASVEFLPVLPSTYCTAIDLKIDRDTGVMHV
                     LLRRKSGDDLRWWAGEIPGWGKGPKNIGIGEVGDTAFALASRPGLVAVCGAKPVATID
                     EVDGLVVLLRPNQPAEERLFDYVPGIDIHTFAETIRDCTFVDDTLVLVGGSYGQHGGD
                     NIRRERLTVIEYDVSTDHDVWTVAGPGPGVQSRALAVDADDEGRYHLAGYTCLDVCAP
                     EGEVRVYEHGGKLVSQVALGPLGSDWLGPHDIAWSPAGYAVVALGEQQGQSLVFKVQA
                     FAPGVPEPLWTFTPNNKQALQIAFAVAVGPFGEIYAGGIGPSFAVIGG"
     gene            complement(1337..2494)
                     /locus_tag="VGB85_14870"
     CDS             complement(1337..2494)
                     /locus_tag="VGB85_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135364.1"
                     /translation="MKLLLPLLVLVVLGACQSPAADTSLFTTTPVITSVSDGSSTGST
                     SSASSSGTTTTTGESGSGDGDTHAEPPRDLGTMPDFGDGAPAGCAGKIDFLFMISRSA
                     LMAERQAQLALAVPQFIDTIKTKFADFDYHIMVVDGDGPPPGAWWGSSTCNEVCPNLD
                     CKVGEDCCPITNSNLDGMTCCDDPSYPCDKLDLVTACDEAWGAGTVFPAGKYTADKPC
                     PIDGGRRYLVTGQTDLDETFECIARVGKSGYDLLGQAFTAAMQEDINGPGGCNEGFLR
                     KDALLMVTFIATNPDDPGPLGSKGTPAGWAKAVLDAKHGDKNSVVMFNIGGCDCGPHE
                     GECHPADRLCVLSAMFPYHHSEYGGVVDYGPAFDKAASLVETACAGFVVPG"
     gene            complement(2731..>3229)
                     /locus_tag="VGB85_14875"
     CDS             complement(2731..>3229)
                     /locus_tag="VGB85_14875"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0135365.1"
                     /translation="DARAADALTRRHLAPLRQFLRTKVRPDDVEDLLQQVWLALGETQ
                     GRAGGADIRGTLRGYLFGIARHVLFAHIRAKHRSAAVDPIESSIAALDPSLSQAVGEQ
                     LAARRMMLALQRLPLDTQVLLELRYFQAMSTAELAAMYEVPVGTIKSRLSQARKLLDT
                     ATKLT"
BASE COUNT          497 a         1058 c         1159 g          515 t
ORIGIN      
        1 catcgacaag aatggtttga ttgaggtcgc ggtcacggcg gagcacgcgg acggcgtgcg
       61 gatggagatc gacaccggcg agccgatcga gctgacgccg accggccctg gcgcgttcgg
      121 cgggcagatc ccggtgttca ccggactcga caatggcaag cactcggcgt ttttaacccc
      181 gtggcgcgac acgctcgtcg gcgagtccgt cgcggcgaac tacgtgatcg ccctgccgcc
      241 gccgggctac gaggtcgcgt ggcagacgac ggacaccgag gggcaggtcg tggcgatcga
      301 cgtgctgccg gacggtcggc cggtcgagct cggcacctac gaggagatgg gcgagccgcg
      361 ctgctacctg caactgcgcg agaagaacgg ggcgtcggtc gagttcttgc ccgtactgcc
      421 atccacgtac tgcaccgcga tcgacctcaa gatcgaccgc gacaccggcg tgatgcacgt
      481 gctgctcagg cgcaagagcg gcgacgacct gcggtggtgg gccggcgaga tccccgggtg
      541 gggcaaaggc ccgaagaaca tcggcatcgg cgaggtcggc gacacggcgt tcgcgctggc
      601 gagccggccc ggcctggtgg cggtctgtgg cgcgaagccg gtcgcaacga tcgacgaggt
      661 cgacgggctc gtcgtgctgc tgcggccgaa ccagccggcg gaggagcggc tgttcgacta
      721 cgtgcccggg atcgatatcc acacgttcgc cgagaccatc cgcgactgca ccttcgtgga
      781 cgacacgctc gtgctcgtcg ggggatcgta cggccagcac ggcggggaca acatacggcg
      841 tgagcgcctc accgtcatcg agtacgacgt ctccaccgac catgacgtgt ggaccgtggc
      901 cggacccgga cccggcgtcc agagccgcgc cctcgcggtc gacgcggacg acgagggccg
      961 ctaccacctc gccggctaca cctgcctcga cgtctgtgcg ccggagggcg aggtcagggt
     1021 ctacgagcac ggcggcaagc tcgtcagcca ggtcgcgctc ggcccactcg ggtcggactg
     1081 gttgggcccg cacgacatcg cgtggagccc ggcgggctac gccgtcgtcg ccctcggcga
     1141 gcagcagggc cagtccctcg tgttcaaggt gcaggcgttc gcgcccggcg tcccggagcc
     1201 gctgtggacc tttaccccga acaacaagca ggccctgcag atcgccttcg cggtggcggt
     1261 gggcccgttc ggcgagatct acgccggagg tatcggcccg tcgttcgccg tcatcggcgg
     1321 gtagggcccg gggctttcag cccggcacca caaaaccggc gcacgcggtc tcgaccaggc
     1381 tcgcggcctt gtcgaacgcg ggcccgtagt cgacgacccc gccgtattcg ctgtggtggt
     1441 atgggaacat cgcgctcagc acacagagcc ggtccgcggg gtggcactcg ccctcgtgcg
     1501 gtccgcagtc gcatccgccg atgttgaaca tcaccaccga gttcttgtcg ccgtgcttgg
     1561 cgtcgagcac cgccttggcc cacccggccg gcgttccctt ggagccgaga ggccccggat
     1621 cgtccgggtt ggtggcgatg aacgtcacca tcaacagcgc gtccttgcgc aggaacccct
     1681 cgttgcagcc gccggggccg ttgatgtcct cctgcatcgc ggcggtgaac gcctgcccga
     1741 gcaggtcgta gccgctcttg ccgaccctgg cgatgcactc gaaggtctcg tccagatcgg
     1801 tctggcccgt cacgaggtag cggcgaccgc cgtcgatcgg acagggcttg tccgccgtgt
     1861 acttgccggc ggggaacacc gtgccggcgc cccacgcctc gtcgcaggcg gtcacgagat
     1921 cgagcttgtc gcatgggtag ctcggatcgt cgcagcacgt catgccgtcc aaattggagt
     1981 tcgtgatcgg gcagcagtcc tcgccgacct tgcagtcgag gttcgggcag acctcgttac
     2041 aggtgctgct gccccaccag gccccgggcg gggggccgtc gccgtccacg accatgatgt
     2101 gataatcgaa gtcggcgaac ttcgtcttga tcgtgtcgat gaactgcggc accgcgaggg
     2161 cgagctgggc ctggcgctcc gccatgagcg ccgaccgcga gatcatgaac aggaagtcga
     2221 tcttgcccgc gcagccggcg ggcgcgccgt cgccgaagtc gggcatcgtg ccgagatcgc
     2281 gaggcggctc ggcgtgcgta tcgccgtcgc ccgagccgct ctcgcctgtc gtcgtggtcg
     2341 tcccggagct gctcgcgctc gaagtcgagc cggtcgacga tccgtccgac acggacgtga
     2401 tcacgggcgt cgtcgtgaac agggacgtgt ccgcggccgg cgactgacag gcgccgagga
     2461 caacgagcac gagcagcgga aggaggagct tcacggcatg gacgataacg cgcctcgggc
     2521 ctgatagcca ccctctgtag tactgctggg cgcgtgtttt atttcccgac taccgaactg
     2581 ggcggactgc tcgggggggt cgcgggcgca gggttgtgag caggtcagtt gccggtacct
     2641 gcggatggtc tgctgccagt ggcgagccgc tgtccgtccc ggccgagccc tgcgtatata
     2701 tatatatata tatatatata tatataatga tcaggtcagc ttcgtcgccg tgtcgaggag
     2761 ctttcgtgcc tgcgacaggc ggctcttgat cgtgccgacg gggacctcgt acatcgcggc
     2821 gagctcggcg gtcgacatcg cctggaagta gcgcagctcg agcaggacct gggtgtcgag
     2881 cgggaggcgc tggagggcga gcatcatgcg ccgggccgcg agctgctcgc cgacggcctg
     2941 cgagagcgag gggtcgaggg ccgcgatcga ggactcgatc gggtcgacgg cggcgctgcg
     3001 gtgcttcgcg cggatgtgcg cgaacaatac gtgccgtgcg atcccgaaca ggtagccgcg
     3061 cagggtcccg cggatgtccg cgccgccggc acggccctgg gtctcgccga gcgcgagcca
     3121 gacctgctgc agaaggtcct cgacgtcgtc gggccgaacc ttggtccgca ggaactggcg
     3181 cagcggggcg aggtgccggc gggtgagcgc gtccgcggcc cgggcgtcg
//
LOCUS       DASWNJ010000880         2453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_686629, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000880 DASWNJ010000000
VERSION     DASWNJ010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686629"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VGB85_14880"
     CDS             complement(<1..601)
                     /locus_tag="VGB85_14880"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEY0135366.1"
                     /translation="MSGAKDRVLVTGGTGKTGGAIARRLTELGLEVRIASRSARGAGQ
                     VRFDWADASTHAAALDGVTRVYLVAPGLVDDPAAMMVPFIKRALASEVRRLVLLGSSA
                     ISDDAPGLGEVARVLRASAPEWAVLRPSWFMQNFLDPAHAHGQTALADGTIVTSTAGG
                     RVGFVDVADIAEVGARALADERSHDAAHVITGPQALSYAE"
     gene            complement(601..1017)
                     /locus_tag="VGB85_14885"
     CDS             complement(601..1017)
                     /locus_tag="VGB85_14885"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0135367.1"
                     /translation="MHTDKQGDAERALNAHLEHITRDMDRWIALFADDAVVEFPYAPS
                     LGRAHALVGKPAIDDYFRGTPGVFRDLQFSGLRRHAAADPEVAIAEVHGSAILAPTGK
                     RYEQDYVMIVRTRGGLIVEYREYWNPLPFLRAMEAT"
     gene            1079..1957
                     /locus_tag="VGB85_14890"
     CDS             1079..1957
                     /locus_tag="VGB85_14890"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0135368.1"
                     /translation="MAAPIDLNLVRAFVGVHETGSFSAAAQRLGVPRSTVSRAVATLE
                     QATGTLLFHRTTRSVTTTPAGIALFDRVAPSLAALDASLAELPETIEAPRGTLRLTTS
                     ADLGMTLLAEAVARFTARHPEVRVEVHLGRAIVDLVRERFDLALRFSPGPLRDSSLVA
                     RRLGPVVFALYAAPQYLARRGAPRSLTDLHAHESVGLGGAPDSPLPDPRTTCDDKFFA
                     REVLRAGAGIGLLPTYLADADLASGALVRVLPRWELRRGAVYLVQPGRKHVPARVTAF
                     RELLLEVMRQRPLAAG"
     gene            complement(1961..>2453)
                     /locus_tag="VGB85_14895"
     CDS             complement(1961..>2453)
                     /locus_tag="VGB85_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135369.1"
                     /translation="HFTPALKADDTPVKCKNGEDYGIGLLSWVAPAWAEPVHGVYSQQ
                     SSMTERRVWICMGYEGFVGCTTHLSTNGETALAQCHDIIDGPLATAAAQGPAVMAGDW
                     NLKFKGSPNAQDCVPGGFFRKGDGGVQHLIASDHFGFIETITIDMKGTTDHPGLEVRL
                     TLP"
BASE COUNT          315 a          968 c          810 g          360 t
ORIGIN      
        1 cctccgcgta gctcagcgcc tgcggcccgg tgatcacgtg cgccgcgtcg tgcgatcgct
       61 cgtcggccag cgcccgcgcc ccgacctcgg cgatgtccgc gacatcgacg aagccgacgc
      121 gcccgcccgc ggtcgaggtg acgatcgtgc cgtccgccag cgccgtctgg ccgtgcgcgt
      181 gcgcggggtc gaggaagttc tgcatgaacc acgacggccg cagcaccgcc cactccggcg
      241 cgctcgcacg cagcacccgc gcgacctcgc cgagccccgg cgcgtcgtcg gagatcgccg
      301 acgacccgag caacaccagc cgccgcacct cgctcgccag cgcccgcttt ataaagggca
      361 ccatcatggc cgcggggtcg tcgacgaggc ccggcgcgac gagatacacg cgggtgaccc
      421 cgtcgagcgc cgcggcgtgg gtcgaggcat cggcccagtc gaagcgcacc tgcccggccc
      481 cccgcgccga acgactggcg atccgcacct cgagcccgag ctcggtgagc cgccgcgcga
      541 tcgcaccgcc ggtcttgccg gtgccgccgg tgaccagcac ccgatccttt gcgcccgaca
      601 tcacgtcgcc tccatcgcgc gcaggaacgg cagcgggttc cagtattcgc ggtactcgac
      661 gatcagcccg ccgcgggtgc gcacgatcat cacgtagtcc tgctcgtagc gcttgccggt
      721 cggggcgagg atcgccgagc cgtggacctc cgcgatcgcc acctcgggat cggccgcggc
      781 gtgcctgcgc agccccgaga attgcaggtc gcggaacacg cccggcgtgc cgcgaaagta
      841 gtcgtcgatc gccggcttgc cgacgagcgc gtgcgcgcgg ccgagcgagg gcgcgtaggg
      901 gaactcgacc accgcgtcgt cggcgaacag cgcgatccag cggtccatgt cgcgggtgat
      961 gtgctcgagg tgggcattga gcgcccgctc ggcatcgcct tgtttgtccg tgtgcatgcg
     1021 gtcatcatgc cgccggtcca cccttgcaac aatgtccacg aagtggcatc tgtgttgcgt
     1081 ggctgcacca atcgatctca acctcgtccg cgcgttcgtc ggcgtgcacg agaccggcag
     1141 cttctcggcc gccgcgcagc ggctcggggt cccgcgctcc accgtgagcc gggcggtcgc
     1201 cacgctcgag caggcgaccg gcacgctgct gtttcatcgc accacccgca gcgtgacgac
     1261 cacccccgcc ggcatcgccc tgttcgaccg tgtggccccc tcgctcgcgg cgctcgacgc
     1321 ctcgctcgcc gagctgccgg agacgatcga ggccccgcgc ggcaccctgc gcctgaccac
     1381 cagcgccgac ctcggcatga cgctgctcgc cgaggccgtc gcccgcttca ccgcgcgcca
     1441 cccggaggtg cgcgtcgagg tccacctcgg ccgcgcgatc gtcgacctcg tgcgcgagcg
     1501 cttcgacctc gcgctgcgct tctcccccgg cccgctgcgc gactcctcgc tcgtcgcgcg
     1561 ccggctcggc cccgtcgtgt tcgcgctcta cgccgcgccg cagtacctcg cgcgccgcgg
     1621 cgccccgcgc agcctcaccg acctgcacgc gcacgagtcc gtcggcctcg gcggcgcccc
     1681 ggactcgccg ctgcccgacc cgcgcacgac ctgcgacgac aagttcttcg cgcgcgaggt
     1741 cctgcgcgcc ggcgccggca tcggcctgct gccgacctac ctcgccgacg ccgacctcgc
     1801 cagcggcgcg ctcgtgcgcg tgctgccgcg ctgggagctg cgccgcggcg cggtgtacct
     1861 cgtgcagccg ggtcgcaagc acgtgccggc gcgggtgacc gcgtttcgcg agctgctgct
     1921 cgaggtcatg cgccagcgcc cgctcgccgc cggctgaggc tcacggcagc gtcaggcgaa
     1981 cctcgagccc cggatgatcg gtcgtgccct tcatgtcgat cgtgatcgtc tcgatgaagc
     2041 cgaagtggtc cgacgcgatc aggtgctgca cgccgccgtc gcccttgcga aagaacccgc
     2101 ccggcacgca gtcctgcgcg ttcgggctgc ccttgaactt gaggttccag tcgcccgcca
     2161 tcaccgcggg gccctgcgcc gcggccgtcg ccagcggccc gtcgatgatg tcgtgacatt
     2221 gcgcgagcgc ggtctccccg ttggtgctca aatgcgtggt gcagccgacg aagccctcgt
     2281 agcccatgca gatccacacc cgccgctcgg tcatgctgct ctgctgcgaa tacacgccgt
     2341 gcacgggctc ggcccacgcc ggcgcgaccc acgacagcag gccgatgcca taatcctcgc
     2401 cgtttttgca cttcaccggc gtatcgtcgg ccttgagcgc cggcgtgaag tga
//
LOCUS       DASWNJ010000881         3086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_16555, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000881 DASWNJ010000000
VERSION     DASWNJ010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16555"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..1718
                     /locus_tag="VGB85_14900"
     CDS             57..1718
                     /locus_tag="VGB85_14900"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEY0135370.1"
                     /translation="MRALRLFLPFALWPLCAEEAAAAPCDCDHVLGLDVTVADGTALG
                     VQPGDSVCVQGGARPFLRLQKFKGAEGQVVEIRNCDGQVDISNDDKGYALTIGQSSFV
                     HLTGTGDDAFEYGFRARGSKDGPDYSAMSVTIGDLSSDIEVDHVEAYEAGFAGFIVKT
                     DPRCDGSANLGKFVMYNVELHHNYIHDTKGEGIYFGSTGYGGREYTCDGEKVLLYPHE
                     HHGAKIHHNLIENTGWDGAQIGVTPKDCEFHHNTIRKIGLAGEKFQQQGLQIGGASAC
                     EIYANILMDGPTNGIFILGADDTRVYNNLIVGFADNGIYANDQQLDLEARYRFAFNTI
                     VQSGEAGITVFGAKLGPGHAYSNVVVGPNPISVGNDVVDFVEMANQTADGPDALGFVD
                     AATRDFHLLADSPLRDVGVAAPELDLQDDLDGVARNDLLPDAGAFEFDLDPVPETDTD
                     ASTGDASTGSVSTGETGADPTTGATGPTLTGTGDADSGTPTTGDATTAPDSPQTTGDT
                     DPSGSSSTGDTGQTSDGGCGCRGADAPLAGWLALLMLAPRRRRAR"
     gene            1772..2377
                     /locus_tag="VGB85_14905"
     CDS             1772..2377
                     /locus_tag="VGB85_14905"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0135371.1"
                     /translation="MPKLSYFDLAGSRGEECRLALHLAGVAFEDDRIKDWPARKQQTP
                     WGTVPTFEVEGRGVLGQSSAILLYIGREHGLHPSDPWQAALHEDLMGAVEDLRSTARP
                     LLHITDPEQRIKAREEFAAGYLKRWGQAVERRLIELGAGPFTAGADIQVVDLKLYMIV
                     RWFASGTVDHVPRDVFAEFPRLTGVYQAVAEHPKIVAWYAR"
     gene            2461..2913
                     /locus_tag="VGB85_14910"
     CDS             2461..2913
                     /locus_tag="VGB85_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135372.1"
                     /translation="MDATTGDPRNPLRRLFDAVIFVVQLPFNPAVVLALMMLLGFQFP
                     GNPLLLEYIGPNEYRPSTAGACILIVLCVVLSLVLLRWRLGRWWATDAVTPWMLPANA
                     VAAGALSIWISSGLEGNLIAFAFMPPLGLTTLYWFYWLGRAPKASAKP"
BASE COUNT          490 a         1113 c         1019 g          464 t
ORIGIN      
        1 tgagcgcgcc gcatcgcgct ttgacagccg gacgcgcctg gcggacgctg cctggcatgc
       61 gtgcgctgcg tctgttcctc cccttcgcgt tgtggccgct gtgcgccgag gaggccgccg
      121 cggccccgtg cgactgcgac cacgtgctcg gcctcgacgt cacagtcgcc gacggcacgg
      181 cgctcggcgt ccagccgggc gacagcgtgt gcgtgcaggg cggtgcgcgg ccattcctgc
      241 gcttacaaaa gttcaagggc gcggagggcc aggtcgtcga gatccgcaac tgcgacggcc
      301 aggtcgacat cagcaatgac gacaagggct acgcgctgac gatcggtcag tcgagcttcg
      361 tgcacctgac cggcacgggg gacgacgcgt tcgagtacgg ctttcgtgcc cgcgggtcca
      421 aggacgggcc cgactactcg gcgatgtcgg tgacgatcgg ggacctgagc agcgacatcg
      481 aggtcgacca cgtcgaggcg tacgaggccg ggttcgccgg cttcatcgtc aagaccgacc
      541 cgcgctgcga cggctcggcg aacctcggca aattcgtgat gtacaacgtc gagctgcatc
      601 acaactatat tcacgacacc aagggcgagg gcatctactt cggcagcacc ggctacggcg
      661 gccgcgagta cacctgcgac ggcgagaagg tgcttctata cccgcatgag catcacgggg
      721 cgaagatcca ccacaacctg atcgagaaca ccggctggga cggcgcgcag atcggcgtca
      781 cgcccaagga ctgcgagttc caccataata cgatccgcaa gatcgggctc gccggggaga
      841 agtttcaaca gcagggcctg cagatcggcg gcgcctcggc gtgcgagatc tacgcgaaca
      901 tcctgatgga cgggccgacc aacggcatct tcatcctggg cgccgacgac acgcgcgtct
      961 acaacaacct gatcgtcggc tttgccgaca acgggatcta cgccaacgat caacagctcg
     1021 acctcgaggc ccgctaccgc ttcgcgttca acacgatcgt gcaatccggc gaggccggca
     1081 tcaccgtgtt cggcgccaag ctcggccccg gccacgcgta cagcaacgtc gtcgtcgggc
     1141 ccaacccgat cagcgtcggc aacgacgtgg tcgacttcgt cgagatggcc aaccagaccg
     1201 ccgacggtcc ggacgcgctc ggcttcgtcg acgccgccac ccgcgacttc catctgctcg
     1261 ccgactcgcc gctgcgcgac gtcggcgtcg cggcgcccga gctcgatctg caggacgacc
     1321 tcgacggcgt cgcgcgcaac gacctgctgc cggacgccgg tgccttcgag ttcgacctcg
     1381 accccgtgcc ggagaccgac accgacgcga gcaccggcga cgcgagcacc ggctccgtga
     1441 gcaccggtga gacgggcgcg gaccctacca ccggcgcgac cggcccgacg ctcacaggca
     1501 cgggagacgc ggactccggg accccgacca ccggcgatgc gacgaccgcc ccggacagcc
     1561 cgcagacgac gggcgacacc gatccatccg gcagctcctc cacgggcgac accggccaaa
     1621 cttccgacgg tggttgcggc tgccgcggcg cagacgcgcc gctcgcaggc tggctcgcgc
     1681 tgctgatgct cgcgccacgg cgtcgtcgag cccgctgaat cctggtcgac cggctgtccg
     1741 atcgaccgag atcggagata atctgccggc catgcccaag ctcagctact tcgacctcgc
     1801 cggcagccgc ggcgaggagt gccgcctcgc cctccatctc gccggtgtcg ccttcgagga
     1861 cgaccggatc aaggactggc ccgcgcgcaa gcagcagacc ccgtggggta cggtgccgac
     1921 cttcgaggtc gagggccgcg gcgtgctcgg ccagagcagc gcgatcctcc tctacatcgg
     1981 ccgcgagcac gggctgcacc cgagcgaccc gtggcaggcc gcgcttcacg aggacctgat
     2041 gggcgcggtc gaggacctgc gcagcacggc caggccgctg ctgcacatca ccgacccgga
     2101 gcagcgcatc aaggcccgcg aggagttcgc cgccggttat ctcaagcgct ggggtcaggc
     2161 cgtcgagcgc cgcctcatcg agctcggcgc tgggcccttc acggccggcg cggacatcca
     2221 ggtcgtcgac ctcaagctgt acatgatcgt gcgctggttc gcgtccggca ccgtcgacca
     2281 cgtgccccgc gacgtgttcg ccgagttccc gcggctgacc ggcgtctacc aggcggtcgc
     2341 cgagcacccg aagatcgtcg cctggtacgc ccgctgaggg cccgtcaggg cgcgcgcgat
     2401 cacggtcacc tccggcctct ggcgcgcgcg cgctcgatcg gcgacgatat ctacacgatg
     2461 gtggacgcga cgacagggga cccgcgcaac ccgctgcggc ggctgttcga cgccgtgatc
     2521 ttcgtcgtgc agctaccgtt caacccggcg gtcgtgctcg cgctgatgat gctgctcggc
     2581 tttcagttcc cgggtaatcc gctgctgctc gagtacatcg gccccaacga gtaccgcccc
     2641 tcgaccgccg gcgcgtgcat cttgatcgtg ctgtgcgtgg tgctgtcgct ggtgctgctg
     2701 cgctggcgcc tcgggcggtg gtgggcgacg gacgccgtca cgccgtggat gctgccggcc
     2761 aacgccgtcg ccgccggtgc cctgtcgatc tggatctcga gcgggctcga gggcaacctg
     2821 atcgccttcg ccttcatgcc tccgctcggg ctgacgacgc tgtactggtt ctactggctg
     2881 ggccgcgccc ccaaagcgtc agcaaagccg tagcgccgac gcgccgcgct cggctatcct
     2941 ccgcgccatg cagcggtttc gctccctcgc ggacatccag gccttcctcg acgccaccga
     3001 ctacagcgac gaaaagttct atcgcagtcc ggcgcgggtg ctggtcgacc ggcgggccca
     3061 ctgcgtcgat ggtgcgctgt tggccg
//
LOCUS       DASWNJ010000882         5734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_299387, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000882 DASWNJ010000000
VERSION     DASWNJ010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299387"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VGB85_14915"
     CDS             complement(<1..301)
                     /locus_tag="VGB85_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135373.1"
                     /translation="MTRVVATVGVLALSVLVAGCTEPVAAPPPTRPLLPTLEPAVDQN
                     PDPTIVEVQFESIAAEVDYRGDGARTAVAAYRDTGRPASTATVPGPLIVANVGDTV"
     gene            complement(340..1749)
                     /locus_tag="VGB85_14920"
     CDS             complement(340..1749)
                     /locus_tag="VGB85_14920"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HEY0135374.1"
                     /translation="MRLALTVPFALLAVAACGVDAEPVPLVAPLGPVPLVDIDPDPDA
                     IEVTIIAGPAQWSFLPDKPADVWAYRDGNADGAAGRVPGPMLLGKVGDRVVVNFRNEL
                     PEATTVHWHGLRVHAGHDGSNVSQMGIEPGETFKYEFTLTDAGTFWFHPHLAADEQIE
                     RGLYAPIVVLGGEAVPVDTDRMFVLDDVKVESTGKLSETITALDLMVGRQGNVLLVNG
                     EKLPRIDAPAGARERWRLLNTANGTFFNVTLPGHKLRVIAWDGGLLPEPYEVDDLVIA
                     PGERYEVLVELGGAEGDELELQTLHYDRGHDLPDLGARTIATIALKAPPTNPAPALPE
                     RWGKAAALPITAETPRRAFLLTEEEAEGPDDEPRFFINDRAYPDVEPLMVGADALEVW
                     EITNDATMDHPFHLHGTFFQVLDQPHLGWKDTVNVPQKSTVRFAVAFDNPGLWMYHCH
                     ILEHTERGMMGVLQVGDSP"
     gene            complement(2032..3399)
                     /locus_tag="VGB85_14925"
     CDS             complement(2032..3399)
                     /locus_tag="VGB85_14925"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY0135375.1"
                     /translation="MKARYLLIPLVTLGCASVKPTESRRDVGELVEGRLGLPAMIPEE
                     QDAESRARVRARVAELMAEPLTPDRALQVAMLNNRELRATLEELGVAQAELVQAGLLQ
                     NPVISGDLVISTKGNGLGGGLALSQSLLSVFLIPAKKRMARTRLQHTVVMVGQAALSL
                     ARDVRVAFVTAQATEQAVALHRSRAQAAEVAHELAQRQFDAGNITPLDQQLFAAALDR
                     ARVELADAQLAATVTREELTRLLGLWGEDVAWAFAAPLESALPPETDLAQLEQQGMRD
                     RLDLSAARYETQSIEYALALRRRSMLPDLNVGVTARNEVGDDVGHEWVLGPSLSLTLP
                     IFDPGHADIARIQAILRQAQHRLQDAAIHVRSEIRVHRAELVAARRKVEYYERTVLPR
                     AESITELTLREYNAMLVGTYQLLETRTDQIEAHQEYVEALRDYWVARSELELAVGGRL
                     PSGGP"
     gene            3436..3777
                     /locus_tag="VGB85_14930"
     CDS             3436..3777
                     /locus_tag="VGB85_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135376.1"
                     /translation="MAPDSVGGFFGCAWWLVCPCGGAGAGVDLLPPLGPWFIPSCPVG
                     AAGVGLDLLPAPDPWFIPSCPVGAAGVELGLFPVLGPWFMPSCPVGAVGVARLLAAPW
                     FIPSWPVCGST"
     gene            complement(4009..5289)
                     /locus_tag="VGB85_14935"
     CDS             complement(4009..5289)
                     /locus_tag="VGB85_14935"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HEY0135377.1"
                     /translation="MTLASREANAAAPAHKWEPSYSGGPDSAARPPGEAVRDYLPTVT
                     PNGATLPWKIVDGVKVYHLVAEEVEHEFAPGLRALCWGYNGRVHGPTIEAVEGDRVRI
                     YVTNKLVAPTTVHWHGIFLPNGMDGVAGLNQRTIAPGETFRYEWTFKQYGTFMYHSHH
                     DEMTQMALGMMGMIVVHPRRPPAGYRVDRDFVILLSEWKVVPGAKRPDPNEMTDFNVL
                     TMNAKAYPGTAPLVCRKGERVRIRFGNLSAMDHHPIHMHGHYFKVVATDGGPIPVSAQ
                     WPEATVLVPVGTTRDIEFVADAPGDWALHCHMTHHVMNQMGHDLPNMIGVDTSKVGAN
                     ASRLVPGYMPMGQHGMGDMAEMGMPVPKNSIPMVGAKGPFEYITMGGMFTILKVRDED
                     IGDENKDPGWYKNPPGTVADLARKEDMDRDGIKA"
     gene            complement(5377..>5734)
                     /locus_tag="VGB85_14940"
     CDS             complement(5377..>5734)
                     /locus_tag="VGB85_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEY0135378.1"
                     /translation="VVAMAGDGINDAPALAKADVGVAMGTGTDVAMNSAQVTLVKGDL
                     RGIAQARRISVDTIANMKQNLGFAFVYNALGIPLAAGLLYPITGWLLSPMIAALAMSL
                     SSASVITNALRLRGAR"
BASE COUNT          805 a         1866 c         2063 g         1000 t
ORIGIN      
        1 ccacggtgtc gccgacattg gcgacgatca gcggtcccgg gaccgtcgcg gtcgatgccg
       61 ggcgtccggt gtcccgatag gccgccaccg cggtgcgggc cccatcgccg cggtagtcga
      121 cctcggccgc gatagactcg aactgcacct cgacgatcgt cgggtcgggg ttttggtcga
      181 ccgcgggctc gagcgtgggg agtagcgggc gggtcggagg aggcgccgcg acaggctcgg
      241 tacagcccgc gaccaggaca gagagcgcaa ggacccccac ggtagcgacg acgcgcgtca
      301 caagtgactg cgtcgcggcc agcgagggtc ggcgctcgtt cacggtgaat cgcccacctg
      361 aagcacgccc atcatgccgc gctccgtgtg ctcgaggatg tggcagtggt acatccacag
      421 tcccgggttg tcgaaggcga ccgcgaaccg gacggtcgac ttttgcggca cgttgacggt
      481 gtccttccag ccgaggtgcg gctggtcgag cacctggaaa aaggtgccgt gcagatggaa
      541 cgggtggtcc atcgtcgcgt cgttggtgat ctcccagacc tcgagcgcgt cggcgccgac
      601 catgagcggc tcgacgtcgg ggtacgcccg gtcgttgatg aaaaagcgcg gctcgtcatc
      661 cgggccctcg gcctcctcct cggtcagcag gaaggcgcgg cgcggcgttt cggcggtgat
      721 cggcagcgca gccgccttgc cccagcgctc gggcaatgcg ggcgccgggt tcgtcggtgg
      781 ggccttcagg gcgatcgtgg cgatcgtgcg cgcaccgaga tccgggaggt cgtggccgcg
      841 gtcgtagtgc agggtctgca gctcgagttc gtcgccctcg gcgccgccga gctcgacgag
      901 cacctcgtag cgctcgcctg gcgcgatcac caggtcgtcg acctcgtacg gctcggggag
      961 cagcccgccg tcccaggcga tcacgcgcag cttgtgtccc ggcaaggtga cgttgaagaa
     1021 cgtgccgttg gcggtgttga gcaggcgcca ccgctcgcgc gcgcccgcgg gagcatcgat
     1081 gcgcgggagc ttctcgccgt tgaccaggag cacgttgccc tggcgaccga ccatcaggtc
     1141 gagggcggtg atcgtctcgg agagcttgcc ggtcgactcg accttgacgt cgtcgagcac
     1201 gaacatgcgg tcggtgtcga cggggaccgc ttcgccgccg agcacgacga tcggcgcgta
     1261 caggccgcgc tcgatctgct cgtcggcggc gagatgcggg tggaaccaga aggtgccggc
     1321 gtcggtcagc gtgaactcgt atttaaaagt ctcgcccggc tcgatgccca tctgcgagac
     1381 gttgctgccg tcgtgcccgg cgtggactcg gagcccgtgc cagtgcacag tcgtcgcctc
     1441 tggcagctca ttgcggaagt tgacgacgac gcggtcgccg accttgccga gtagcatcgg
     1501 ccccgggacg cggcccgccg cgccgtcggc gttgccgtcg cggtacgccc agacgtcggc
     1561 gggcttgtcc ggaaggaacg accactgcgc cgggccggcg atgatggtga cctcgatcgc
     1621 gtccgggtcc ggatcgatgt cgacgagcgg caccgggccg agaggagcca ccagcggcac
     1681 cggctctgca tcgacgccac aggcggccac ggccaggagg gcgaacggta cggtcagggc
     1741 gaggcgcatc tttagggctt tcggggtcgg accgatgatg gttgctgaaa ttcttggact
     1801 tgtcgctgcc gggcgactgg gcgcgaaagt gcggcgtatc ggggtgaggt gggtgcgtca
     1861 ccagcatcgc ccgtgccaga cgtctcccgc atcgccagcg ttaccaattt cgaggtcaca
     1921 tggccgtcct cggggcgcac tggagagtat tcgcgagtga cctgagacta tcgtgaagtc
     1981 tctcgaggcc gttgtggccg tggacgtccc cgtggtcggg gggaagcgtc ctcacggacc
     2041 tccgctcggc aagcgaccgc caacggcgag ttccagctcg ctgcgagcga cccagtaatc
     2101 acgcagggcc tcgacgtact cctggtgagc ttcgatctgg tcggtgcggg tctcaaggag
     2161 ctggtaagtg ccgaccagca tcgcgttgta ctcgcgcaac gtcagctcgg tgatcgactc
     2221 ggcgcgcggc agcaccgttc gctcgtaata ctcgaccttg cggcgcgccg cgacgagctc
     2281 cgcccggtgc acgcggatct cggatcgcac gtggatcgcc gcgtcctgca ggcggtgctg
     2341 ggcctgccgc aggatcgcct ggatgcgggc gatgtcggcg tggcccgggt cgaagatcgg
     2401 cagcgtgagc gagagcgatg gaccgagcac ccactcatga ccgacgtcgt cgccgacctc
     2461 gttgcgggcg gtgaccccga cgttgaggtc cggcaacatg ctgcggcgac gcagcgcgag
     2521 ggcgtactcg atcgactgcg tctcgtagcg cgccgcggac agatcgagcc ggtcccgcat
     2581 gccctgctgc tcgagctggg ccaggtcagt ctcgggcggc aaggcgctct cgagcggcgc
     2641 cgcgaaggcc cacgcgacgt cctcacccca caggccgagc aagcgcgtca gctcctcgcg
     2701 agtgacggtc gcggccagct gcgcgtcggc gagttcgacc cgcgcgcgat cgagggcggc
     2761 cgcaaagagc tgctggtcca gcggggtgat gttgccggcg tcgaactgtc tctgggcgag
     2821 ttcgtgggcg acctcggccg cctgggcgcg gctgcggtgg agcgcgaccg cctgttcggt
     2881 cgcctgagcc gtgacaaagg cgacccgcac gtcgcgtgcg agcgacagcg ccgcctgccc
     2941 caccatcacc acggtgtgct ggaggcgggt cctggccatg cgtttcttgg ccgggatcag
     3001 gaacacgctc aggaggctct gagacagcgc gaggccgcca ccgaggccgt tgcccttcgt
     3061 cgagatgacg aggtcgccgg agatcaccgg gttctgcaac agacccgcct gcacgagctc
     3121 ggcctgggcg acgccgagtt cttcgagcgt ggcgcgcagc tcccggttgt tgagcatcgc
     3181 tacctgcaac gctcgatcgg gggtcaacgg ctcggccatc agctcggcga cgcgggctcg
     3241 cacgcgggcg cggctctccg cgtcctgttc ctccggaatc atggccggta ggccgaggcg
     3301 gccctcgacg agctcgccga cgtctcgtcg tgactccgtg ggcttgaccg aggcgcagcc
     3361 gagcgtgacg agggggatca ataaataacg agccttcatc gcggcggctc cgcctgcttg
     3421 tgcatgttcg gggacatggc gccggattcc gtggggggct tctttggctg cgcctggtgg
     3481 ctggtgtgtc cttgcggggg agcgggtgcc ggcgtcgact tgctgccgcc gctgggtccg
     3541 tggttcatcc cctcatgtcc cgtcggcgcg gccggtgtgg ggctcgactt gctgccggca
     3601 ccggacccgt ggttcatccc ctcgtgtccc gtcggcgcag cgggtgtcga gctcggctta
     3661 tttccggtgc tcggcccatg gttcatgccc tcgtgccccg tgggcgcggt cggcgtggcc
     3721 cgtttgctgg cagcgccgtg gttcataccc tcgtggcccg tgtgcggatc gacctgagag
     3781 ccgtggttca tgttgccgtg tcccgcgtgc ggatcgacct tggccggctg ctcgcctgcg
     3841 aacgccgacg tgtcgtaggg gttcgcctgg acctggtagg gcgcggctgc ggcgttggga
     3901 tcggctgcgt cggccccggg gggctcagga ggcagcccgc cgcggcaccc ggcgagttgc
     3961 gtgaccagca gcgttgagag tagcggccgc gttcggccgc gcctgggatc aagccttgat
     4021 gccgtcacgg tccatgtcct ccttcctggc gaggtcggcg acggtgcccg gcgggttctt
     4081 gtaccagcct gggtctttat tctcgtcgcc gatgtcctcg tcgcgcacct tgaggatcgt
     4141 gaacatgccg cccatcgtga tgtattcgaa gggccctttg gcgccgacca tcgggatgct
     4201 gttcttcggg acaggcatgc ccatctcggc catgtcgccc atgccgtgct gccccatcgg
     4261 catgtaaccc ggcacgaggc ggctggcgtt ggcgccgacc ttgctggtgt cgacgccgat
     4321 catgttgggg aggtcgtgcc ccatttggtt catgacgtgg tgggtcatgt gacagtgcag
     4381 cgcccagtcc ccgggcgcgt ccgccacgaa ctcgatgtcg cgcgtggtgc ccacgggcac
     4441 caggacggtc gcctccggcc actgcgccga tacggggatc gggccaccgt cggtcgccac
     4501 gaccttgaag tagtggccgt gcatatggat cgggtgatga tccatggcgc tgagattgcc
     4561 gaaccggatc cgcacgcgct cgcccttgcg gcacaccagc ggcgccgtgc cggggtacgc
     4621 cttggcgttc atcgtgagga cgttgaagtc ggtcatctcg ttcgggtccg gccgcttggc
     4681 cccggggacg accttccact cgctgagcag gatcacgaag tctcgatcga cgcggtagcc
     4741 cgcgggcggg cggcgcggat ggacgacgat catgcccatc atgcccagcg ccatctgcgt
     4801 catctcgtcg tggtgcgagt gatacatgaa ggtgccgtac tgcttgaagg tccactcgta
     4861 gcggaaggtc tcgcccgggg cgatggtccg ctgattgagc ccggcgaccc cgtccatgcc
     4921 gttggggagg aagatgccgt gccagtggac cgtcgtcggc gcgacgagct tgttggtcac
     4981 gtagatgcgt acgcggtcac cctcgaccgc ctcgatcgtc gggccatgaa ccctgccgtt
     5041 gtagccccag cacagcgcgc gcagcccggg ggcgaactcg tgttcgacct cctcggcgac
     5101 gaggtgatag accttcacgc cgtcgacgat cttccatggc aacgtcgctc cgttcggcgt
     5161 gacggtcggc aagtagtcgc gtactgcctc acccgggggg cgcgcggcgg agtcggggcc
     5221 gccgctgtag ctcggctccc acttgtgggc aggcgcagcg gcgttcgctt cgcggctcgc
     5281 cagagtcacg gcggcgccgc cgagcgttgc gccgcccaga atggccaggc gccgggtaat
     5341 tttaggttct gtcatgacgc tccgatcgtg gtgggctcat cgcgctccgc gcagccgtag
     5401 cgcgttggta atgaccgagg ccgagctcag gctcatggcg agggcagcga tcatcggtga
     5461 gagcagccag cccgtgatgg gatatagcag gccggccgcc agcggaatgc cgagggcgtt
     5521 gtagacgaac gcaaaaccca ggttctgctt catgttggcg atggtgtcga ccgagatgcg
     5581 gcgcgcctgg gcgatgcccc gcaagtcgcc cttcacgagc gtgacctggg cgctattcat
     5641 cgccacgtcg gtgcccgtgc ccattgcaac gccgacgtcg gcctttgcca gagccggggc
     5701 gtcgttaatg ccgtcgccgg ccatggcgac gacc
//
LOCUS       DASWNJ010000883         2958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_228078, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000883 DASWNJ010000000
VERSION     DASWNJ010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228078"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..981
                     /locus_tag="VGB85_14945"
     CDS             142..981
                     /locus_tag="VGB85_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135379.1"
                     /translation="MSLARLTSARRSLLLVWAGGLAATALGSVVVALVSLRLGLFLAT
                     LAVEVAAVYMAFAGRDRLALDDPGRRVWLWIGVGLALRLVAELRLATLYLDVVPHFVT
                     DNPWVWAVYFFGLRYLYTVADLALLAALLATRKALLSTGLDFHLRPRDAMVLVLLPPL
                     PVTVYLLQSVFLSASTDPGLHTFRIISATVGAVVSGVCVVLASPALQMGGGAWAWIWG
                     AAAVAGIARALAFAAAAVAPTFPGAVLVEQALLWTFACGWLLATVLHLRLITHERGVG
                     VRP"
     gene            complement(982..1740)
                     /locus_tag="VGB85_14950"
     CDS             complement(982..1740)
                     /locus_tag="VGB85_14950"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY0135380.1"
                     /translation="MPLERNGANATAAVHVGCSGWIYAHWRGLFYPQKLKSAQWFEYY
                     ARHFDTVEINNTFYRLPEATVFAAWREQAPPGFIYAVKASRYLTHMKKLKDPAEPLAR
                     LLDRAVHLKDHLGPILYQLPPGWKADAARLDAFLSQLPKDRTHVLEFRDESWYVDEIL
                     ALVRSHGASLCVHDMPGRAPERMALGPIAYVRFHGPTGQYFGSYDADALRPWAEWLAR
                     QRDAGRDIYVYFNNDIGGHAVRDAVRLREMLAAL"
     gene            1815..2948
                     /locus_tag="VGB85_14955"
     CDS             1815..2948
                     /locus_tag="VGB85_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135381.1"
                     /translation="MRRACTLLAGSLTLACGSGAPITGSDAGGDTTAATATTGTGSTG
                     ATGATDATGTGDPPTTGATGGASETGTTDPLTGGAESTSTSGAGSSTGGQTDTGTGET
                     GPAETGDTGPAETGVVDACPCPDLVVGLDDGIFVLSKTAVLWKYFPVKNELAMLGAVD
                     CGLPPSTFSMGVDREGSAWVQFSDKQIRKVDITDVSKCSDPGFVPGQDNIGNFGMAFV
                     SNSAEDSCDRLHGNHYNFKAEGDMVAEFFSVDPDTLALEQLGKSNYGLAEVTGTGDGR
                     AFLFAGPDPSRLVEVDKATGATKSVLPLPGVKTGGGFAFAFFAGDFYFFTDAESDGSS
                     EVTHLDFDDSDKNGMQDLKVVLEDAPLRIVGAGVSTCAPVIPQ"
BASE COUNT          411 a         1031 c         1045 g          471 t
ORIGIN      
        1 ggcccagaag gacgtgccgc gcccgcagtt ctcgatcacg ctgcggccgc gcgggggcgt
       61 gcgcgcgacc ttgcaccgcg ccgggccggc ctgaccggcc ggcttgacgt gcttcggccc
      121 cgaaccgcac gctagggccg cttgtctctc gcccgcctca cctccgcgcg tcgctcgctg
      181 ctcctcgtgt gggccggagg gctggcagcg accgcgctcg gcagtgtggt ggtcgcgctc
      241 gtatcgctgc ggctgggtct gttcctggcg acgctcgcgg tcgaggtcgc cgcggtctac
      301 atggccttcg ccggccgtga ccggctcgcg ctcgacgacc cgggccggcg cgtgtggctg
      361 tggatcggtg tcggcctcgc cctgcgactg gtcgccgagc tgcgcctggc gaccctgtat
      421 ctcgacgtcg tcccgcactt tgtcaccgac aacccgtggg tatgggcggt gtacttcttc
      481 ggcctgcgat atctctatac cgtcgccgac ctggcgctgc tcgccgcgtt gctggcgacc
      541 cgcaaggccc tgctgagcac cggcctcgac ttccacctgc ggccgcgcga cgcgatggtg
      601 ctcgtgctgc tgccgccgct gccggtgacc gtctatctcc tgcagtcggt gttcctgtcg
      661 gcgtcgaccg acccgggtct gcacaccttt cgcatcatca gcgccaccgt cggcgcggtc
      721 gtcagcggcg tgtgcgtcgt gctcgccagc ccggcgctgc agatgggcgg cggcgcctgg
      781 gcgtggatct ggggcgccgc cgcggtcgcg ggcatcgccc gcgcgctggc cttcgccgcc
      841 gccgcggtcg cgccgacctt ccccggggcc gtgctcgtcg agcaggcgct gctgtggacc
      901 ttcgcctgcg gctggctgct cgccaccgtg ctgcacctgc gattgatcac ccacgagcgc
      961 ggcgtcggcg tccgcccgtg atcacagcgc cgcgagcatc tcgcgcaggc gcaccgcgtc
     1021 gcggaccgcg tggccgccga tgtcattatt aaaatatacg tagatgtcgc ggccggcgtc
     1081 gcgctggcgg gcgagccact cggcccacgg gcgcagcgcg tcggcgtcgt agctgccgaa
     1141 gtactggccg gtcgggccgt ggaagcggac gtacgcgatc gggccgaggg ccatgcgctc
     1201 gggggcccgg ccaggcatgt cgtgcacgca caggctggcc ccgtgcgacc gcaccagcgc
     1261 gaggatctcg tcgacgtacc aggactcgtc gcgaaactcg agcacgtgcg tgcggtcctt
     1321 cggcagttga ctcaagaagg cgtccaggcg cgccgcgtcg gccttccacc ccggcggcag
     1381 ctgatacagg atcggcccca ggtgatcttt taaatggacg gcgcggtcga gcaggcgcgc
     1441 gagcggctcg gcggggtcct tgagcttctt catgtgggtg agatatcggc tggccttgac
     1501 cgcatagata aagcctggcg gggcctgctc gcgccaggcc gcgaacacgg tcgcctcggg
     1561 caggcgataa aaggtgttgt taatttcgac ggtgtcgaag tgccgcgcgt agtattcgaa
     1621 ccattgcgcg gacttcagct tttgcgggta gaacaggccg cgccagtggg cgtagatcca
     1681 gccggagcag ccgacatgga cggcagccgt ggcgttcgcg ccgttgcgct cgagaggcac
     1741 ccgaccacca tgcgcgatct ggcgacacac gcacgtcgcg ggaccaccgc cgcgacatgg
     1801 attatcctgt cgggatgcga cgcgcgtgca ctctcctcgc cggctccctg accctcgcgt
     1861 gcggctctgg agccccgatc accggctcgg acgcgggcgg cgacacgacg gccgcgaccg
     1921 cgacgaccgg gacgggctcg acgggcgcga ccggggcgac cgacgcgacc ggcaccggcg
     1981 acccgccgac caccggcgcg accggcggag ccagcgagac cggcacgacg gacccgctca
     2041 ccggcggtgc ggagagcacc agcacgagcg gcgcgggatc gtcgacgggc gggcagaccg
     2101 acaccggcac gggcgagacc gggccggcgg agacgggcga caccgggccg gccgagacgg
     2161 gcgtggtcga cgcgtgcccg tgcccggacc tcgtcgtcgg cctcgacgac ggcatcttcg
     2221 tgctctcgaa gacggccgtg ctgtggaagt actttcctgt caaaaatgag ctggcgatgc
     2281 tcggggcggt ggattgcggg ctgccaccgt cgacgttctc gatgggggtc gatcgtgagg
     2341 gatccgcgtg ggtgcaattc agtgacaagc agatccgcaa ggtcgacatc accgacgtca
     2401 gcaaatgctc ggaccccggg tttgtgcccg gtcaggacaa tatcggtaac ttcgggatgg
     2461 cgttcgtgtc caactcggcc gaggactcct gcgatcgcct ccacggcaat cattacaact
     2521 tcaaggccga gggtgacatg gtcgccgagt tcttcagcgt cgaccccgac acgctcgcgc
     2581 tcgagcagct cggcaagagc aactacgggc tcgccgaggt caccggcacc ggggacggcc
     2641 gcgccttcct gttcgccggg ccggacccct cgcggctggt cgaggtcgac aaggcgaccg
     2701 gcgcgaccaa gagcgtgctg ccgctgcccg gcgtgaagac gggcggcggc ttcgcgttcg
     2761 cgttcttcgc cggcgacttc tacttcttca ccgacgccga gtccgacggc agcagcgagg
     2821 tcacccacct cgacttcgac gacagcgaca agaacggcat gcaggatctc aaggttgtcc
     2881 tcgaggacgc gccgctgcgg atcgtcggcg cgggggtctc gacctgcgcg ccggtcatcc
     2941 cgcaatgacg gtcactga
//
LOCUS       DASWNJ010000884         4176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_581495, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000884 DASWNJ010000000
VERSION     DASWNJ010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581495"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VGB85_14960"
     CDS             <1..722
                     /locus_tag="VGB85_14960"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0135382.1"
                     /translation="PVIHIDGISKQHGKQILFIETSATVFRGEKVGLVGPNGAGKTTI
                     FRMIMHTETPDEGQVRVDKSVTLGYFSQDVGEMAGRSVVAETMDGAGPIAQIAQELGE
                     LNEALADPARMHEMDALIARYGEVQARFEELDGYGLDARAREILAGLGFAQDVMDNDV
                     GKLSGGWKMRVALARILLLRPDVLLLDEPTNHLDLESILWLEQYLRDFQGALIMTSHD
                     REFLNRLVTKIVEFEGGEMIT"
     gene            738..920
                     /locus_tag="VGB85_14965"
                     /pseudo
     CDS             738..920
                     /locus_tag="VGB85_14965"
                     /inference="COORDINATES: protein motif:HMM:NF024256.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1038..1625
                     /locus_tag="VGB85_14970"
     CDS             1038..1625
                     /locus_tag="VGB85_14970"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0135383.1"
                     /translation="MMVRRHERWCIMGENGAGKSTLLKLIAGQTQPDNGAVTIGAAVK
                     LGYFAQHAMDILDATGTVLETLMAAFPLASIGQLKALAGAFGFSGDDAEKPVRILSGG
                     EKARVVLALMLYDPPNLLVLDEPTNHLDLATKDMLVGALSSFEGTMIFVSHDRRFLAA
                     LSNRVLELGNGPPRLYGGGYTEYVAQSGHEAPGMR"
     gene            complement(1669..2745)
                     /locus_tag="VGB85_14975"
     CDS             complement(1669..2745)
                     /locus_tag="VGB85_14975"
                     /inference="COORDINATES: protein motif:HMM:NF039286.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY0135384.1"
                     /translation="MSSPRPSTAMREQTYPPPYPDGWYRVALSSELRRGDRRYVEALG
                     RQLLVYRSDVDDSVHAMAAFCPHLGANLADGCIKGGRVQCPFHGWELASDGHVAHIPY
                     AKRLPVRMRQPTWPVYERYGQIFVYHRGGCGVDTSAPPPYEFLQIADIDDGRLVFRGK
                     HHAGTVRMHLLEFAENSVDFAHFGPLHGKMFVPWTRFIVPGIVVEHDALWELETDPAR
                     AHIAYFKNNAVLKVRGKYIDRTRASALITFLGPAGVVTFRFTIPGMGEILMFQTHLPR
                     APLEQEVDFHWFAEPKIPRVLVSYVVGNWVSQWRNDVHIWENKIHQARPTLVQEDGPI
                     PRLRRWFSQFYPKQGPDDAASAAE"
     gene            2860..3843
                     /locus_tag="VGB85_14980"
     CDS             2860..3843
                     /locus_tag="VGB85_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135385.1"
                     /translation="MRRPALALLALLACDRGETIGEYRQRIDPELLAACEPRVAKMRE
                     AFARIPREPSYHKTPEDWLPVVHPVGEPLVEDTRWTHRLAVHADGGFGYERAKYRLSA
                     IDQILGEDLVKQCEHARLDRTFEPTLTIWPDRRAPVAELVHLLGKLPQPLRHTLVVYV
                     PADAAPPPPVTPAIAATHGKPLVEELMAVNDAIGEALGVLWWCDPVRAVYDHTSDDSP
                     VTRTVLLDDLPKAILECRCEGIDVETVIAGYWSLYLYRFLRPRVIPLPLTRDPKADAL
                     VVPRDATGTDLIALVAAKGPGPYRLVLAPDPSAPPPEPPEPQRGRHKQPRR"
     gene            complement(3851..>4176)
                     /locus_tag="VGB85_14985"
     CDS             complement(3851..>4176)
                     /locus_tag="VGB85_14985"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY0135386.1"
                     /translation="LGIGTMVGWRRIVVTVGEKIGKDHLTYAQGAAAELVAMSTIGIS
                     AGLGLPVSTTHVLSSGIAGTMVAQRSGLQLNTVTKIASAWLLTLPVTMVLAGVLFLVF
                     RAMLA"
BASE COUNT          674 a         1503 c         1381 g          618 t
ORIGIN      
        1 cccccgtgat ccacatcgac ggcatctcga agcagcacgg caagcagatc ctcttcatcg
       61 agacctcggc gacggtgttt cgtggcgaga aggtcggtct ggtcgggccc aacggtgccg
      121 gcaagaccac gatctttcgc atgatcatgc acaccgagac cccggacgag ggtcaggtgc
      181 gcgtcgacaa gagcgtcacg ctcggatact tcagccagga cgtcggcgag atggccggcc
      241 gcagcgtcgt cgccgagacg atggacggcg ccggcccgat cgcccagatc gcgcaggagc
      301 tcggcgagct caacgaggcg ctcgccgacc cggcgcgcat gcacgagatg gacgccctga
      361 tcgcccgcta cggcgaggtt caggcccgct tcgaggagct cgatggctac ggcctcgacg
      421 cccgcgcgcg cgagatcctc gccggcctcg gcttcgcgca ggacgtcatg gacaacgacg
      481 tcggcaagct gtccggcggc tggaagatgc gcgtcgcgct ggcgcggatc ctgctgctgc
      541 gccccgatgt cctgctgctc gacgagccga ccaaccatct cgacctcgag tcgatcctgt
      601 ggctcgagca gtacctgcgc gactttcagg gcgcgctgat catgacgagc cacgaccgcg
      661 agttcctcaa ccgcctcgtg accaagatcg tcgagttcga gggcggcgag atgatcacct
      721 aagtcggcaa ctacgacttt tacgagcagc aacgcgcgat cggcgaggcc caggcgcagg
      781 cccagtacga gcgccagcag gcgatgctcg ccaaggagat ccgcttcatc gagcgcttca
      841 aggcccaggc cgggcgggcc gcgcaggtgc agtcgcgggt caagaagctc gacaagatcg
      901 agaaggtcga gccgccgcgc cggcgcaggg tcctcagctt cgactttcgt cccgcgccgc
      961 gatccggcaa cgatgtcatc aagctcagcg ggatccgcaa ggcctacgga aagaagatca
     1021 tttatgatgg cttcgacatg atggtccgcc gccacgagcg gtggtgcatc atgggcgaga
     1081 acggggccgg caagagcacc ctgctaaagc tcatcgccgg gcagacccag ccggacaacg
     1141 gcgctgtcac gatcggcgcg gctgtcaagc tcgggtactt cgcgcagcac gcgatggaca
     1201 tcctcgacgc gaccggcacc gtgctagaga ccctgatggc cgcgttcccg ctggcctcga
     1261 tcggccagct caaggcgctc gcgggcgcgt tcggcttctc gggcgacgac gccgagaagc
     1321 cggtgcggat cctctccggc ggcgagaagg cgcgcgtcgt gctcgcgctg atgctctacg
     1381 acccgccgaa cctgctcgtg ctcgacgagc cgaccaacca tctcgacctg gcgaccaagg
     1441 acatgctcgt cggcgccctg tcgagcttcg agggcacgat gatcttcgtg tcccacgacc
     1501 gtcgcttcct cgccgccctg agcaaccgcg tgctcgagct cggcaacggc ccgccgcgcc
     1561 tgtacggcgg cggctacacc gagtacgtgg cccagagcgg ccacgaggcc cccggcatgc
     1621 gctaaacggc tcgctgccgc gtcgcctact ccgctgccga cgcgtcgcct actccgctgc
     1681 cgaggccgcg tcgtccggcc cctgcttggg atagaactgc gagaaccacc ggcgcagccg
     1741 cgggatcggg ccgtcttctt gcacgagggt cggccgcgcc tggtggatct tgttctccca
     1801 gatgtggacg tcgttgcgcc actgcgagac ccagttgccg accacgtacg agaccagcac
     1861 ccgcgggatc ttcggctcgg cgaaccagtg gaagtcgacc tcctgctcga gcggcgcgcg
     1921 aggtaaatgc gtctggaaca tcaagatctc acccatgccc gggatcgtga agcggaaggt
     1981 gacgacgccc gccgggccca aaaaggtgat cagcgccgac gcccgcgtgc ggtcgatgta
     2041 tttgccgcgc accttgagca ccgcattgtt cttgaaatat gcgatgtggg cgcgcgccgg
     2101 gtcggtctcc agctcccaca gcgcgtcgtg ctcgacgacg atgccgggca cgatgaagcg
     2161 cgtccacggc acgaacatct tgccgtgcag cggcccgaag tgcgcgaagt cgacgctgtt
     2221 ctccgcgaac tcgagcaggt gcatgcgcac cgtcccggcg tggtgcttgc cgcggaacac
     2281 cagccggccg tcgtcgatgt ccgcgatctg caggaactcg tatggcggcg gcgcgctggt
     2341 gtccacgccg cagccgccgc ggtgatacac gaagatctgg ccgtaacgct cgtacaccgg
     2401 ccaggtcggc tggcgcatgc gcaccggcag gcgcttcgcg taggggatgt gcgccacgtg
     2461 cccgtcgctc gccagctccc agccgtggaa cgggcactgc acccgcccac ccttgatgca
     2521 gccgtccgcg aggttggccc cgaggtgcgg acaaaaggcc gccatcgcgt gcacgctgtc
     2581 gtcgacgtcg ctgcggtaca ccagcagctg tcgcccgagc gcctcgacgt agcgccggtc
     2641 cccgcggcgc agctcgctcg acagcgcgac gcggtaccac ccgtccggat acggcggtgg
     2701 ataggtctgc tcgcgcatgg cggtcgaggg tcggggcgac gacatggccg cactgtagcg
     2761 cgcgcgccgt gggtcgctcg cggcgatctt tcgtcgcctc ggcttttcgc gcgaaagcgc
     2821 gatcacgggc cccgcggcgg cccgtgatat cgtcccacga tgcgtcggcc agccctcgcg
     2881 ctcctcgcac tgctcgcctg cgaccgcggc gagacgatcg gcgagtaccg gcagcgcatc
     2941 gatccggaat tgctggccgc gtgcgagccc agggtcgcca agatgcggga ggccttcgcc
     3001 cggatccccc gcgagccgtc ctatcacaag acccccgagg actggctgcc ggtcgttcac
     3061 cccgtcggcg agccgctcgt ggaggacacc cggtggaccc accgtctggc cgtgcacgcc
     3121 gacggcggct tcggctatga gcgcgccaag taccggctca gcgcgatcga ccagatcctc
     3181 ggcgaggacc tcgtcaagca gtgcgagcac gccaggttgg atcggacctt cgagccgacc
     3241 ctgaccattt ggcccgacag gcgagcgccc gtcgccgagc tcgtgcacct gctgggcaag
     3301 ctgccccagc cgctgcgcca cacgctggtc gtctacgtgc cggccgacgc cgccccgccg
     3361 ccgccggtga ccccggcgat cgcggccacc cacggcaagc ccctggttga agagctcatg
     3421 gccgtcaacg acgcgatcgg cgaggcgctc ggcgtattgt ggtggtgtga ccccgtccgc
     3481 gccgtctacg atcacacgag cgacgacagc ccggtcaccc gcacggtcct gctcgacgac
     3541 ctgccgaagg cgatcctcga gtgtcgctgc gagggcatcg acgtcgagac cgtgatcgcc
     3601 ggttactggt cgctgtacct ctatcgattc ttgcgcccgc gcgtcattcc actcccgctg
     3661 acgcgcgacc cgaaggccga cgccctcgtc gtcccgcgcg acgcgaccgg caccgacctc
     3721 atcgcgctcg tcgcagcaaa gggccccggc ccgtaccgcc tggtgctcgc gcccgacccc
     3781 tcggctccgc ccccggagcc gcccgagccg cagcgcgggc gccataaaca gccgcgccgc
     3841 tgaccggcgt tcacgccagc atcgcgcgga acaccaggaa cagcacgccg gcgagcacca
     3901 tcgtgaccgg cagggtcagc agccaggccg acgcgatctt ggtgaccgtg ttgagctgga
     3961 ggcccgagcg ctgggcgacc atcgtgccgg cgatgccgga cgacagcacg tgggtcgtgc
     4021 tgaccggcag gcccaggccc gccgagatcc cgatcgtgct catcgccacc agctcggcgg
     4081 cggccccctg cgcgtaggtc aggtggtcct tgccgatctt ctcgccgacg gtgacgacga
     4141 tgcgccgcca gccgaccatc gtgccgatgc cgagcg
//
LOCUS       DASWNJ010000885         1615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_547093, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000885 DASWNJ010000000
VERSION     DASWNJ010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547093"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VGB85_14990"
     CDS             complement(<1..391)
                     /locus_tag="VGB85_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEY0135387.1"
                     /translation="MADLSSRRQRILFALCRDYIVTGRPVASASLTQHLEWSSATIRN
                     ELAALEEQGLLHQPHQSAGRVPSAAGMRLYVASLGTLPPLRPEVRRAVDVSFGDVVHG
                     PEGMRAGVRVLSELVGCVAVTFVGEGRR"
     gene            complement(541..>1615)
                     /locus_tag="VGB85_14995"
     CDS             complement(541..>1615)
                     /locus_tag="VGB85_14995"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HEY0135388.1"
                     /translation="AGLERELDARLALGASAKGHVPQDSGVVPPGDAWPTVYLGGGTP
                     SLLGPDGLRALGEFIARRFPGAGGEEYTVEVNPEHADRALYAAVRSIGGTRVSLGVQT
                     LDPAGLGALGRAHDAATAKTALICALEAELAVSADLIVGWPGQSNEALAADVTQLVDL
                     GVRHVSVYALTIESGTPWETLVRRGKRRLPDPDHQADMLLAAEAALTGAGLEHYEVAS
                     YAAPGERARHNLGYWTWQDYLGLGPSAHSAVYAADGSVTRRGNLRGLPAWQADPATPA
                     EIDPQDPTAAAAEGLWTGLRHLPGLDLAAFLARFPAVDRAWIEGRTRKQVARGNLELA
                     GGVLRVAAGRWMWHDAIAADLLA"
BASE COUNT          208 a          601 c          605 g          201 t
ORIGIN      
        1 cgcgccgacc ctccccgacg aaggtcaccg cgacgcagcc gaccagctcg ctgagcaccc
       61 gcaccccggc gcgcatgccc tcgggcccgt gcacgacatc accgaacgag acatcgacgg
      121 cgcgccggac ctcgggtcgc agcggcggca aggtcccgag cgacgcgaca tacaggcgca
      181 tgcccgcggc gctcggcacc cgccccgccg actgatgcgg ctgatgcagc aggccctgct
      241 cctcgagcgc ggccagctcg ttgcggatcg tcgccgacga ccactcgagg tgctgcgtca
      301 ggctcgcgga ggccacggga cgacccgtga cgatgtagtc gcggcacagg gcgaacagga
      361 tccgctgccg ccgggaactc aggtcggcca cggtcgatgg atttagcgcg atcggcccga
      421 acgcgccagc cccctccccg gcgcgctttt gcggctcgcc cgctcccccg tgcgtcggcg
      481 tcgcccgtcg gcggccgcgg ccggcgcagg gtgcccgcac gtgctggtcg tgcgaaagcg
      541 tcaggccagc aggtcggccg cgatcgcgtc gtgccacatc cagcgcccgg cagcgacgcg
      601 caggacaccg ccggcgagct cgaggttgcc tcgcgcgacc tgcttgcgcg tccgcccctc
      661 gatccacgcg cggtcgaccg cgggaaagcg ggcgaggaag gccgcgaggt cgagccccgg
      721 caggtggcgt aatccggtcc acaggccctc ggcggcggcc gccgtcggat cttgcggatc
      781 gatctccgcg ggcgtggccg ggtcggcttg ccaggccggg aggccgcgca ggttgccgcg
      841 gcgggtcacc gaaccgtcgg cggcgtacac ggcggagtgc gccgaggggc cgaggccgag
      901 gtagtcctgc caggtccagt agccgaggtt gtggcgcgcg cgctcgcccg gcgccgcgta
      961 gctggcgacc tcgtagtgct cgaggcccgc gccggtcagc gccgcctcgg cggcgagcag
     1021 catgtcggcc tggtgatcgg ggtcgggcag gcgtcgcttg ccgcggcgca ccagcgtctc
     1081 ccatggcgtg ccgctctcga tcgtcagcgc gtagaccgac acgtggcgca cgccgaggtc
     1141 gaccagctgc gtgacatcgg ccgcgagcgc ctcgttcgac tgtcccggcc agccgacgat
     1201 caggtcggcg ctgaccgcca attcggcctc cagggcgcag atgagggctg ttttggcggt
     1261 cgcggcgtcg tgggcccggc cgagcgcgcc gaggccggcg gggtcgaggg tctgcacgcc
     1321 gagcgacacg cgcgtgccgc cgatcgagcg tactgcggca tacagcgccc ggtcggcgtg
     1381 ctccgggttg acctcgaccg tgtactcctc gccgccggcg cccgggaagc ggcgcgcgat
     1441 gaattcgccg agcgcgcgca ggccgtcggg gccgagcaag ctcggggtgc cgccgccgag
     1501 gtacacggtc ggccacgcgt cgccgggcgg cacgacgccg ctgtcctgag ggacatgtcc
     1561 cttcgcactt gccccgagag ccaggcgcgc gtcgagctca cgctcgaggc cggcg
//
LOCUS       DASWNJ010000886         5707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_327697, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000886 DASWNJ010000000
VERSION     DASWNJ010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327697"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..2689)
                     /gene="lon"
                     /locus_tag="VGB85_15000"
     CDS             complement(233..2689)
                     /gene="lon"
                     /locus_tag="VGB85_15000"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEY0135389.1"
                     /translation="MARKIPLLPLRELIVFPHEVVPLFVGREKSINALEEAMATDRAI
                     LLCAQKKAKVNDPRPEGIHNFGTIGSIIQLLRLPDGTVKVLVEGKSRARIGRYIDHEK
                     YFLVEADVLEPPVVSAEDEPEIAALMRSVQQTFENYVKLNKRVPPELAVSVQSIDNPS
                     RMADTIAAHVNFKLTAKQELLETESIRARLEKLYELMQNEIEILQVEKKIRTRVKKQM
                     EKNQKEYYLNEQMQAIQKELGSQDEFKNEISELEERVRRRKMSAEASDRLKKELRKLK
                     MMSPMSAEATVVRNYIDTVLALPWDERTVEKLDIAEAEGILDADHYGLEKPKERIIEY
                     LAVRSLVPKLKGPILCLVGPPGVGKTSLAKSVARATNRKFVRVSLGGVRDEAEIRGHR
                     RTYIGAMPGKIIQGLRKAASSNPVFLLDEIDKISMDFRGDPSAAMLEVLDPEQNNTFV
                     DHFLDLDYDLSDVLFLCTANSLHSIPLPLQDRMEIIELSGYTDEEKQKIARQYLIPKQ
                     LEANGMQNAEIVFTDQAVRELIHHYTKEAGVRSLERAVSSVVRKLAREFVKDGKKKKS
                     FKVDVRLVQKLLGAQKYRYNSADAADQIGMTNGLAVTIFGGDLLPAEVSLTAGKGKVT
                     LTGKLGDVMQESAQAAVTYVRTRSLSFGLEPDFHEHVDFHVHFPEGAVPKDGPSAGVT
                     IVTSLVSALLQVPVRKDVAMTGEINLRGKVLPIGGLKDKVLAAFRGEMKTVICPRDNE
                     KDLADVPKHVLKALDIKLADTIDDVLRWALVSLPEDHPNVALRKFLAGADVTAPVDWR
                     EVNTYLEERRRKDRAAHDVH"
     gene            complement(2933..4075)
                     /locus_tag="VGB85_15005"
     CDS             complement(2933..4075)
                     /locus_tag="VGB85_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135390.1"
                     /translation="MSGPALVLLPDPEARVDPGVPVLGVAPAVRLADAARAAGFAAIY
                     AAAGVRTHPAGATLASAGDRVDTAALVLLESVAVEPGALARLVHASGEGSLFDARGRP
                     VGWWTTRLGRLPAVLPVGEALASPEAPAPGELARLVDPEDRPQVEALIVRAVAGEPRF
                     GAVMAAAAGSPWRGWLELPVLRWLCARGRPIARVELLALLLAAVAGVPALLDSRLGLV
                     LAAALLLVAVQLAALLPELRALLRPGTASAAGWLAPAIRPFGHASLAAALTYDLVASP
                     VRTGVADLVLLVLGGAAALLSLAQARAELRGQHPAPFELPSLDGFAARLELALPAWLQ
                     TPVRAEILVFLLAWVGAPGLPWGVLVVIGLARLWRWFIGHPDARLG"
     gene            complement(4146..4850)
                     /locus_tag="VGB85_15010"
     CDS             complement(4146..4850)
                     /locus_tag="VGB85_15010"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY0135391.1"
                     /translation="MSGLPRALLLDLDGTLVDSEMVHVAGLRRLCEDRGVVLTEAEGL
                     YVIGHGWREICRELRLEQRLGLDLAGVIAGTVAAKDRLFGEGLELPVLPGAPELLALA
                     RAAKIPTAIVTGSARPELDQALRALGIASEQLAASVCSEEVAHGKPSPDGYLRAAALL
                     AVPPAACLVVEDSEAGIAAGIAAGMRVLAVAAGNQPAGSPGHQDQRRAHHHRASLVGL
                     DLAAMAAMMTTIATEI"
     gene            4985..>5707
                     /locus_tag="VGB85_15015"
     CDS             4985..>5707
                     /locus_tag="VGB85_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135392.1"
                     /translation="MPVPSTPGDDRCLAGLCAALEASRSEPPERVLAGFAEAVMADLA
                     ACLVLRDGLASARALTRERRAVIECELRLPAAWTAALGEGRIVRASAAELGPGERAAL
                     RLGPTSALLLAPVPAPEGPAQAALLLASDLSHGTWSSGQEQALRGLASGLGGWFAARS
                     LRQVLDHLPQRVTWKDASLRYRGANRAFTRSAGLTTPQLLGRADAELPLRAESGISTE
                     PAAYAEATRREQRALTTPQLHHL"
BASE COUNT          803 a         2094 c         1994 g          816 t
ORIGIN      
        1 ggccacggcc ggctcacgca cgctgcgtgt ccgaacgcgg tggtcagcct tccgacgttc
       61 ttcgcggaca tgctcgaggt ccgcggggcg cagcgctcga gtgcccatgc aatgaaacag
      121 ccccggaccg cgagacggcc ggggcggcgg cctccgagag cctccggagg caaagggcct
      181 tcaggcccaa tcggccgata ggccgaccca ggccccggac ggggccgtga agctaatgca
      241 cgtcgtgggc ggcgcggtct ttgcggcggc gttcttcgag gtacgtgttg acctcgcgcc
      301 agtcgaccgg ggcggtgacg tcggcgccgg cgaggaactt gcgcagggcg acgttggggt
      361 ggtcctccgg gaggctgacg agggcccagc gcaggacatc gtcgatcgtg tcggcgagct
      421 tgatgtcgag ggccttgagc acgtgcttcg ggacgtcggc gaggtccttc tcgttgtcgc
      481 gcgggcagat gacggtcttc atctcgccgc ggaaggccgc gaggaccttg tccttgaggc
      541 cgccgatcgg cagcaccttg ccgcggaggt tgatctcgcc ggtcatggcg acatccttgc
      601 ggaccgggac ctgcagcagc gcgctgacca gcgaggtgac gatcgtgacg cccgcgctcg
      661 ggccgtcctt gggcaccgcg ccctccggga agtgcacgtg gaagtcgacg tgctcgtgga
      721 agtcgggctc gaggccgaag gagaggctgc gcgtgcgcac gtaggtgacg gccgcctggg
      781 ccgactcctg catgacgtcg ccgagcttgc cggtcagcgt caccttgccc ttgccggccg
      841 tcagcgagac ctcggcgggc agcaggtcgc cgccgaagat cgtcaccgcg aggccgttgg
      901 tcatgccgat ctggtcggcc gcgtcggcgc tgttgtagcg gtacttctgc gcgccgagca
      961 gcttttggac cagccgcacg tcgaccttga aggacttctt cttcttgccg tccttgacga
     1021 actcgcgggc cagcttgcgc accaccgagg acaccgcgcg ctcgaggcta cggacgccgg
     1081 cctccttggt gtagtggtgg atcagctcgc gcacggcctg gtccgtgaac acgatctcgg
     1141 cgttttgcat gccgttcgcc tcgagctgct tcgggatcag gtactggcgg gcgatcttct
     1201 gcttctcctc gtccgtgtag ccggagagct cgatgatctc catgcggtcc tgcagcggca
     1261 gcgggatcga gtgcaggctg ttcgccgtgc acaggaacag cacgtcgctc aggtcgtagt
     1321 cgaggtcgag gaagtggtcg acgaaggtgt tgttctgctc ggggtccagc acctcgagca
     1381 tcgccgccga cgggtcaccg cggaagtcca tcgagatctt gtcgatctcg tcgagcagga
     1441 acaccgggtt gctgctcgcg gccttgcgca gaccctggat gatcttgcca ggcatcgcgc
     1501 cgatgtaggt ccgacgatgg ccgcggatct ccgcctcgtc gcgcacgccg ccgagcgaca
     1561 cgcgcacgaa cttgcggttg gtcgcccgcg cgacgctctt ggccagcgag gtcttgccga
     1621 cgcccggcgg gccgacgagg cacaggatcg gccccttgag cttcggtacc agcgagcgca
     1681 ccgcgaggta ctcgatgatg cgctccttcg gcttctcgag gccgtagtgg tcggcgtcga
     1741 ggatgccctc ggcctcggcg atgtcgagct tctcgaccgt gcgctcgtcc cacggcagcg
     1801 cgagcaccgt gtcgatgtag ttcctgacga cggtcgcctc ggcgctcatc gggctcatca
     1861 tcttgagctt gcgcagctcc ttcttgaggc ggtcactcgc ctccgcgctc atcttgcgcc
     1921 ggcgcacccg ctcctcgagc tcggagatct cgttcttgaa ctcgtcctgg ctgccgagct
     1981 ccttctggat cgcctgcatc tgctcgttga ggtagtactc cttctggttc ttctccatct
     2041 gcttcttgac gcgcgtacgg atcttcttct ccacctggag gatctcgatc tcgttctgca
     2101 tcagctcgta gagcttctcc aggcgcgcgc ggatcgactc ggtctcgagc agctcctgct
     2161 tggccgtcag cttgaagttg acgtgcgcgg cgatcgtgtc ggccatgcgc gaggggttgt
     2221 cgatgctctg cacgctgacc gccagctccg gcgggacgcg cttgttgagc ttgacgtagt
     2281 tctcgaaggt ctgctggacc gagcgcatca acgccgcgat ctcgggctcg tcctcggcgc
     2341 tgacgaccgg cggctcgagc acgtccgcct cgacgaggaa gtacttctcg tggtcgatat
     2401 agcggccgat acgggcgcgg ctcttgccct cgacgagcac cttgacggtc ccgtcgggga
     2461 gacgaagcag ctggataatc gagccgatag tcccgaagtt gtggatgccc tcggggcgcg
     2521 ggtcgttgac cttggccttc ttctgggcgc acagcaaaat ggcgcggtcg gtcgccatcg
     2581 cctcctcgag ggcgttgatc gacttctccc gacccacgaa gagcggcacg acctcgtgcg
     2641 ggaagacgat gagttcacga agcggaagta aggggatctt gcgggccatg gcagggtttg
     2701 ccacgcggac ggcgcgcgac cgggcaaagc tagcacccgc aaaaaaccgg gtcaaacgca
     2761 gcgcatgccg gcgactcgcc gcgcgcggcc gctgccgagc gcgacgggcg accctcgacg
     2821 cacgcgcatg cgcagaggca cgcgaacgat cgatggcgcc gcggcgcatc atcgagatcg
     2881 gcaggtgtcc gcgccgggcc tttgggtcag gtcggtgcag gcacgtcaga cgtcagccga
     2941 ggcgagcgtc agggtggccg atgaaccagc gccacagtcg cgcgagcccg atgacgacga
     3001 gcacacccca cggcaatccc ggggcgccga cccaggcgag taaaaacacc aagatctccg
     3061 cgcgtaccgg ggtctgtagc cacgcgggca gcgccagctc gagccgcgcc gcgaagccgt
     3121 cgaggctcgg gagctcgaag ggcgccggat gctgaccgcg cagctcggcg cgggcctgcg
     3181 cgagtgacag cagcgccgcc gcaccgccga gcaccagcaa caccagatcg gcgacccccg
     3241 tccgcaccgg cgaggcgacc aggtcatagg tcagcgcggc cgccaaagat gcgtgcccga
     3301 acggccggat cgccggcgcc agccagcccg ccgccgaggc cgtgcccggt cgcagcagcg
     3361 cccgcagctc cggcagcaag gccgccagct ggaccgccac gagcagcagc gccgccgcga
     3421 gcacgagccc gagccgcgag tcgagcagcg ccggtacgcc cgcgaccgcg gccagcaaca
     3481 gcgcgagcag ctcgacccgc gcgatcgggc gaccccgggc gcacaaccag cgcagcaccg
     3541 gcagctcgag ccagccgcgc cacggcgacc cggccgccgc tgccatcacc gcgccgaagc
     3601 gaggctcgcc cgcgaccgcc cgcacgatca acgcctccac ctgcgggcga tcctcggggt
     3661 cgaccaggcg cgccagctca ccgggcgccg gcgcctccgg cgaagcgagc gcctcaccga
     3721 ccggcagcac cgccggcaac cgcccgagcc gcgtcgtcca ccacccgacc ggccgcccgc
     3781 gcgcgtcgaa caggctgccc tccccgctcg cgtgcaccag ccgcgcgagc gcgccaggct
     3841 cgaccgcgac gctctccagc agcaccagcg cggccgtgtc gacgcgatct cccgcggacg
     3901 cgagggtcgc cccggcgggg tgcgtgcgga cccccgcagc ggcatagatc gccgcgaagc
     3961 ccgccgcccg tgcggcgtcg gcgaggcgca ccgcgggggc gacgccgagc accggcacgc
     4021 ccggatcgac ccgcgcctcg gggtcgggca gcagcaccag cgccggcccg ctcacgccgc
     4081 tcctggtctt cgcgacaggt cctggagggc atgtcggatc ggacagcagg tcgtcggacg
     4141 cacggtcaga tctccgtagc gatcgtcgtc atcatcgctg ccatcgccgc gaggtcgagg
     4201 ccgacgagcg aggcccggtg gtggtgggcc cggcgctggt cctgatgtcc gggagatccc
     4261 gcgggctggt tgccggccgc gaccgccagc acgcgcatgc ccgcggcgat cccggcggcg
     4321 atcccggcct cgctgtcctc gacgacgaga cacgcggccg gcggcaccgc cagcaacgcg
     4381 gcggcccgca ggtacccgtc cgggctcggt ttgccgtgtg cgacctcctc ggagcacacg
     4441 ctcgccgcga gctgctcgct ggcgatgccg agggcccgca gcgcctggtc gagctcgggc
     4501 cgcgccgagc cggtgacgat cgcggtcggg atcttcgccg cgcgggccag cgccagcagc
     4561 tcgggcgcgc cgggcaacac cgggagttcg aggccctcgc cgaacagtcg gtccttggcg
     4621 gcgaccgtgc cggcgatgac cccggcgagg tcgagcccga ggcgctgctc gagccgcagc
     4681 tcccgacaga tctctcgcca gccgtggccg atgacgtaca gcccctcggc ctcggtcagc
     4741 acgacgcccc ggtcctcgca cagccgccgc aacccggcga cgtgcaccat ctcgctgtcg
     4801 acgagggtcc cgtcgaggtc gaggagcagc gcgcgcggca ggccggacat gcgacgcaca
     4861 ctaacgtagc cgccggcccg gcgcgaatct ccggcgagcg acgcgactcg gcggaccgcc
     4921 gaggtcggcg cgggaccccg ggcaccgcgc ccgctcgcgc gcctgttaga ctgccggccc
     4981 cgcgatgccc gtgccctcca cccccggcga cgaccgctgc ctcgcgggcc tgtgcgccgc
     5041 gctcgaggcc tcgcgcagcg agccgcccga gcgcgtgctc gccggcttcg ccgaggcggt
     5101 gatggccgat ctcgccgcgt gcctggtgct gcgcgacggc ctcgccagcg cgcgcgcgct
     5161 gacccgcgag cgccgcgccg tgatcgagtg cgagctgcgc ctgcccgccg cctggaccgc
     5221 ggcgctcggc gagggccgga tcgtccgcgc cagcgcggcc gagctcggcc ccggcgagcg
     5281 cgccgcgctg cgcctgggac cgacgagcgc cctcctgctc gcccccgtgc ccgcccccga
     5341 gggccctgcg caggccgccc tgctgctcgc ctcggacctg tcccatggga catggtcttc
     5401 gggccaggag caggcgctgc gcggcctcgc gtccggcctc ggcgggtggt tcgcggcgcg
     5461 cagccttcgc caggtcctcg accacctccc gcagcgcgtc acctggaagg acgcgagcct
     5521 gcgctaccgc ggggccaacc gcgccttcac gcgcagcgcc ggcctgacga ccccgcagct
     5581 gctcggtcgc gccgacgccg agctgccgct gcgcgccgag tccggcatct ccaccgagcc
     5641 ggcggcgtac gccgaggcca cgcgtaggga acagcgggcc ctgacgaccc cgcagctcca
     5701 ccacctc
//
LOCUS       DASWNJ010000887         4837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_264158, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000887 DASWNJ010000000
VERSION     DASWNJ010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264158"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VGB85_15020"
     CDS             complement(<1..324)
                     /locus_tag="VGB85_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135393.1"
                     /translation="MTPSRPHAPFAARILAFTLAVSALFEMLVMWLQRRAFAGAAAET
                     SYDGRSELFGLLELAGRGGLLAAALLACLAVSAALRYRRDIDDPLARNLALAAAVGLG
                     LEAARQ"
     gene            complement(345..1511)
                     /locus_tag="VGB85_15025"
     CDS             complement(345..1511)
                     /locus_tag="VGB85_15025"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0135394.1"
                     /translation="MTAIRPALQPDLAKYREDYPILAGTTYMNSNSMGAMPRQAADAL
                     AQYAADWAREGVEVWPHWFEVLEATADSAARFLGAPAGETILNQNVAYFQAAVASALD
                     FRGDRNRVVVESLQFPNVLYVWDRYRELGAELSLVPSDDGMTIPLERMLHAIDERTAI
                     VPISHGIYVSGALQDVKAICRRAHEVGALVMVDVYQTVGAVPIDVHDWDADIVVGGSH
                     KWLCGGPGTAFMWMKPALRERLQPRITGWMGHEDPFAFEPVPIRFAAGHRRFLGGTPS
                     IPAYYAARAAFENLHAIGVDRIRAHNLALARIIIDRAQAHGLTIHSPVADEQRTGFVA
                     VDFPDSLAAGKQLIAEHYKLDWRPNCGLRLGPHFYNTEAEVHRMMDRIVALAGG"
     gene            complement(1508..2602)
                     /locus_tag="VGB85_15030"
     CDS             complement(1508..2602)
                     /locus_tag="VGB85_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135395.1"
                     /translation="MPRGRLPVGLAALLLAGAASAAPGPKTSREPPAVEGPAPPPGPK
                     ASPAPEGPAPPPGPKASPASEGPAPPPGPKASNPTPEGPAQLPGPTGARATSERPWLR
                     EAPATGDGEASRGDLGLTRQRDGSRLYVDPGKRFTAAVNPDGSVRFGNRWGRDQHGDR
                     MRGSGRALRTFGPSGIGAVGMALTGPTEWLLALSGQEADAAAKTAFLNATRDLRIQLA
                     IAYTKQLLATRLDELGQELMGLWTDDSRTLAERRAIVFQRWDECDERLVQGLPGGGEL
                     PPEALSAIDDARLAAADRARRNIEGFVRRQMPRGTPRGYTPAELAEFNRRRVSREPFA
                     PYEPRSIDARSARPNTPAPVPTDMSSGAAP"
     gene            complement(2606..3004)
                     /gene="gcvH"
                     /locus_tag="VGB85_15035"
     CDS             complement(2606..3004)
                     /gene="gcvH"
                     /locus_tag="VGB85_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEY0135396.1"
                     /translation="MTESSIPNNLRYTKDHEWARAESDGTITVGITTHAVDQLGDITL
                     VSLPGVGEKVTEGHRFGDVDSVKAVSELFAPIDGEVIAVNEHLTDSPEAVNADPYGVG
                     WMIRLRPRDADALAKLLSAEEYIRHVAENG"
     gene            complement(3041..4168)
                     /gene="gcvT"
                     /locus_tag="VGB85_15040"
     CDS             complement(3041..4168)
                     /gene="gcvT"
                     /locus_tag="VGB85_15040"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEY0135397.1"
                     /translation="MASDNASLQRTPLHALHVARGAKMVDFGGWHMPLQYEGILAEHR
                     AVREAVGIFDVSHMGEIDFTGPGALAAVQRLITNDAAKLVDGKAMYTCVCYPDGGIVD
                     DCIVYRRGAEDLRIVVNASNIAKDFAFFREHAGQLCDIANHSDEVALIAVQGPKARAL
                     VSTLGGEATMQLMQVPSFGFAPGQIAGVDIVAARTGYTGEDGFELFVPAAGGPAVWQA
                     LLDAGAAPIGLGARDTLRLEARLSLYGNDIDQTTSPLEAGLGWVVKLDKLTRGEPVVG
                     AEALAAQKAQGLTRSLVGFKVADRNIARHGAEVVDDAGKVVGQVTSGGPAPTVGGAVG
                     LAYVPLALATAGTPLTISQRGRTFTAEVVKGPFYRRPETAS"
     gene            4337..>4837
                     /locus_tag="VGB85_15045"
     CDS             4337..>4837
                     /locus_tag="VGB85_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135398.1"
                     /translation="MLGLLCGSTGCGAKGNKDEVLPPLGMNTHSQARRYVDKAVRRSS
                     EGVILMPSKKADTLFDRTRLNEIAQSLRVPAAVCFVNRAIETMKPGTQDGEQTYLGVP
                     EGQIRIRTRINPAGEVLRTEVLETGFKDEAMYPCLDKAIKNIKWTTNRSGVVQFIDVI
                     YWVSLGY"
BASE COUNT          656 a         1561 c         1921 g          699 t
ORIGIN      
        1 ctggcgcgcg gcctcgaggc cgaggcccac ggctgccgcg agggccaggt tgcgggccag
       61 ggggtcgtcg atgtcccggc ggtagcgcag cgccgcggag acggcgaggc acgcgagcag
      121 cgcggcggcg aggaggccac cgcggccggc cagctcgagc aggccgaaca gctcgctgcg
      181 gccgtcgtac gaggtctcgg ccgccgcgcc ggcgaaggcg cgccgttgca gccacatcac
      241 cagcatctcg aacagcgccg atacggcgag cgtgaaggcg aggatgcggg cggcgaacgg
      301 ggcgtgcggg cgcgacgggg tcatgctctc gggtccggat cgggtcagcc gccggcgagg
      361 gcgacgatgc ggtccatcat gcgatggacc tcggcctccg tgttgtaaaa gtgcggcccg
      421 aggcgcaggc cgcagttggg ccgccagtcg agcttgtagt gctcggcgat cagttgtttg
      481 ccggccgcga ggctgtcggg gaagtcgacc gcgacgaagc cggtacgctg ttcatccgcg
      541 acgggcgagt ggatcgtcag gccgtgggcc tgcgcgcggt cgatgatgat ccgcgcgagc
      601 gcgaggttat gggcccggat gcggtcgacg ccgatcgcgt gcaggttctc gaacgcggcg
      661 cgggcggcgt agtaggccgg gatcgagggc gtgccgccga ggaagcggcg gtggccggcc
      721 gcgaagcgga tcgggaccgg ctcgaaggcg aaggggtcct cgtggcccat ccagccggtg
      781 atgcgcggct gcaggcgctc gcgcagcgcg ggcttcatcc acatgaacgc ggtcccgggg
      841 ccgccgcaca gccacttgtg gctgccgccg acgacgatgt cggcgtccca gtcgtgcacg
      901 tcgatcggca cggcgccgac ggtctggtac acgtcgacca tcaccagcgc gccgacctcg
      961 tgggcgcggc gacagatcgc cttgacgtcc tggagggcgc cgctgacgta gatgccgtgc
     1021 gagatcggga cgatcgcggt gcgctcgtcg atcgcgtgga gcatgcgctc gagcgggatc
     1081 gtcatgccgt cgtcggaggg caccagcgag agctcggccc cgagctcgcg gtagcggtcc
     1141 cacacgtaga gcacgttggg gaactgcagc gactcgacga cgacgcgatt gcgatcgccg
     1201 cgaaagtcga gcgcgctggc gacggcggcc tgaaagtagg cgacgttctg attgaggatt
     1261 gtttcgcccg cgggagcgcc gaggaagcgc gccgcgctgt cggcggtcgc ctcgagcacc
     1321 tcgaaccagt gcggccacac ctcgacgccc tcgcgcgccc agtcggcggc gtactgcgcg
     1381 agcgcgtcgg cggcctgccg cggcatcgcg cccatgctgt tgctgttcat gtaggtcgtg
     1441 ccggcgagga tcgggtaatc ctcgcggtac ttggccaggt cgggctgcag ggcggggcgg
     1501 atcgcggtca tggggctgct ccggaggaca tgtccgtggg gacaggtgcg ggggtgttcg
     1561 gacgggcgct gcgcgcgtcg atgctgcgcg gctcgtaagg ggcgaagggc tcgcggctga
     1621 cgcggcgacg gttgaactcg gcgagctcgg cgggggtgta gccgcgcggg gtgcccctgg
     1681 gcatctgccg gcggacgaag ccctcgatgt tgcggcgggc ccggtcggcg gccgcgaggc
     1741 gggcgtcgtc gatcgcggag agggcctctg ggggcagctc gccgccgccg ggcaggccct
     1801 gcacgaggcg ctcgtcgcac tcgtcccagc gctggaacac gatcgcgcgg cgctcggcga
     1861 gggtgcggct gtcgtcggtc cacagcccca tcagctcctg gccgagctcg tcgaggcggg
     1921 tcgcgagcag ctgcttcgtg taggcgatcg cgagctggat ccgcaggtcg cgggtcgcgt
     1981 tgaggaaggc ggtcttggcg gcggcgtcgg cctcctggcc cgagagcgcg agcagccact
     2041 cggtcggccc ggtcagcgcc atgccgaccg cgccgatgcc cgagggcccg aaggtccgca
     2101 gcgcccggcc cgagccgcgc atgcgatcgc cgtgttgatc gcgaccccag cggttgccaa
     2161 agcgcaccga gccgtccggg ttgacggccg cggtgaagcg cttgccgggg tcgacgtaga
     2221 ggcgggagcc atcgcgctgg cgcgtcagcc cgaggtcgcc gcggctggcc tcgccgtcgc
     2281 cggtggccgg cgcctcgcgc agccacgggc gctcgctggt cgcgcgggcg ccggtcgggc
     2341 ccggcagctg ggcggggccc tcgggggtcg ggttgctggc ctttgggcca ggtgggggtg
     2401 cggggccctc ggaagcgggg ctggcctttg ggccgggcgg cggtgcgggg ccctcgggag
     2461 cggggctggc ctttgggcca ggtggcggtg cagggccctc gacggccggt ggttcgcggc
     2521 tggtcttcgg gccaggtgcc gcggacgcgg ccccggcgag gagcagggcc gcgaggccga
     2581 ccgggagcct gccgcgcggc atgggtcagc cgttctcggc gacgtgtcgg atgtactcct
     2641 cggcggagag cagcttggcg agcgcgtcgg cgtcacgcgg acgcaggcgg atcatccagc
     2701 cgacgccgta ggggtcggcg ttgacggcct cgggcgagtc ggtgaggtgc tcgttgacgg
     2761 cgatgacctc gccgtcgatc ggggcgaaca gctcggagac ggccttgacg gagtcgacgt
     2821 cgccgaagcg atggccctcg gtcaccttct cgccgacgcc gggcagagag acgagggtga
     2881 tgtcaccgag ctggtcgacc gcgtgggtcg tgatgcccac ggtgatcgtg ccgtcggact
     2941 cggcccgggc ccactcgtga tccttggtgt aacgcaggtt gttggggatg ctgctctcgg
     3001 tcatggatgc gcctgatcgg ggtgagggac agtggacggg tcaggaggcg gtctcggggc
     3061 ggcgatagaa cgggcccttg acgacctcgg cggtgaaggt acgcccgcgc tgcgagatcg
     3121 tgaggggggt tcccgcggtg gcgagcgcga gcgggacgta cgcgaggccg accgcgccgc
     3181 cgaccgtcgg ggccgggccg ccgctggtca cctggccgac gaccttgccg gcgtcgtcga
     3241 cgacctcggc gccgtggcgg gcgatgttgc ggtcggcgac cttgaagccg acgaggctgc
     3301 gcgtcaggcc ctgcgccttc tgggcggcca gcgcctcggc gccgacgacg ggctcgccgc
     3361 gggtcagctt gtcgagcttg acgacccagc cgaggccggc ctcgagcggg ctggtggtct
     3421 ggtcgatgtc gttgccgtag agcgagaggc gcgcctcgag gcgcagggtg tcgcgcgcgc
     3481 cgaggccgat cggcgcggcg ccggcgtcga gcagcgcctg ccacaccgcg ggtccaccgg
     3541 cggccgggac gaacagctcg aagccgtcct cgccggtgta gccggtgcgc gccgcgacga
     3601 tgtcgacgcc ggcgatctgt cccggggcga agccgaagct cggcacctgc atcagctgca
     3661 tcgtggcctc gccgccgagc gtgctgacga gcgcgcgagc cttcgggccc tgcacggcga
     3721 tcagggcgac ctcgtcgctg tggttcgcga tgtcacacag ctgtccggcg tgctcgcgga
     3781 agaacgcgaa gtccttggcg atgttggagg cgttgacgac gatgcgaagg tcctcggcgc
     3841 cgcggcgata gacgatgcag tcgtcgacga tgccgccgtc gggatagcag acgcaggtgt
     3901 acatcgcctt gccgtcgacg agcttcgcgg cgtcgttggt gatcaggcgc tgcacggcgg
     3961 ccagggcgcc cggacccgtg aagtcgatct cccccatgtg cgagacgtcg aagatgccga
     4021 ccgcctcgcg cacggcccgg tgctcggcga ggatgccctc gtattgcagc ggcatgtgcc
     4081 agccgccgaa gtcgaccatc ttggccccgc gagcgacgtg cagggcgtgg aggggggtgc
     4141 gctggaggga ggcgttgtcg ctggccacgg gacgtcacga atagccaatc tcggcccccg
     4201 cgggcaagag cgcgtgctac acctgcgcct tgcgatgacc acgaagcgaa gcgttagcgc
     4261 agcagcgcag agcgcgctga ctatcctggg cggcctgggc ggcctttttg tcctgggcgg
     4321 cctgggcggc ctttttgtgc tgggcctgct gtgcgggtcc acgggttgcg gcgccaaggg
     4381 caacaaggac gaggtcctgc cgcctctggg gatgaatacg cactcccagg cgcggcgtta
     4441 cgtcgacaag gccgtgcggc gctcgtcgga gggcgtgatc ctcatgccat cgaagaaggc
     4501 ggacaccctc ttcgatcgga cccgcctcaa cgagatcgcc cagagcctgc gggtcccggc
     4561 ggcggtgtgc ttcgtcaacc gagcgatcga gacgatgaag cccggcacgc aggacggcga
     4621 gcagacgtac ctcggcgtgc ccgaggggca gatccggatc cgcacccgga tcaacccggc
     4681 gggcgaggtc ctgcgcaccg aggtgctcga gaccggcttc aaggacgagg cgatgtatcc
     4741 ctgcctcgac aaggcgatca agaacatcaa gtggacgacc aaccgcagcg gcgtggtcca
     4801 gttcatcgat gtcatctact gggtcagcct cggctac
//
LOCUS       DASWNJ010000888         5100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_547210, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000888 DASWNJ010000000
VERSION     DASWNJ010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547210"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..985
                     /locus_tag="VGB85_15050"
     CDS             272..985
                     /locus_tag="VGB85_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135399.1"
                     /translation="MQESTSTAPAELAKLSSPAIVARVVAEPSLVKLAGAIFESRATH
                     PRAAEMLSEAYASGKAPPWMTAYLLGCMRATAMYSVVRAILVSAPGLLAESYAGVALV
                     KISGDAAREDLLALLAGPYPRACRDGAMYGLAALRDPSLVAPLLTLARTGQIASKAAA
                     HIIAADNPTPEQLVGWLGSPEASDRSVALRVIVGAAKIDRERKFDPTVVQATRQALGQ
                     ASDLPEYLSTWLRQRLSGF"
     gene            complement(996..3200)
                     /locus_tag="VGB85_15055"
     CDS             complement(996..3200)
                     /locus_tag="VGB85_15055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0135400.1"
                     /translation="MKRLPLALGLLVGSALVFVGGVEPPQVGAAPMDCQKEGNCTFKK
                     PRLAFVLDDSTAMNQAFDADSTRWEAAIEMVGTVVNFDNGFIAENYILGLVRFGHDPN
                     PNVPGTKIVGDTSGLVDGVKLDVPWYDANDPMKAYTECTNGDAIVAALEALPAPLGGA
                     NTGIGAWTHGSLTFTKNYIAQANADHPDDTSKRWAVLMVLTTGAWTNATNTLDNAPPS
                     QDPTLVAKDMWDNLAIPTYVVAFGGPGDKATADALAFAGGTDHAVDAADPQATQLIVD
                     ALNAILIKIKQSLIAPICTPGIPRVMVLLDASSSTLNDGDMRAAPGVGTWDRVREGLA
                     GEGGVFELVLPNNHPAEDMFFFGLSVFGSPGDEKVMIPYGRCHQDNFAWALDPFTSCE
                     APGCTDPYAKPTLMWTFKDGSQIDPPGFDDPTISHMPECNGNQPKGACDGSGNTLHLG
                     LQLVASNLEAYRSACVMDQAPMPCNADTPFLNFLITDGAYDSTDAQVQAPLLAMHEAG
                     VVTHVIGYGANVDPVQLVKLAEWGSGATLPPTIANTQDELEAAFQGFLVLPVFDECCA
                     FQQCDGGGEPMGETGDLSDTDDSFSTGENGGSTAGPNDTSTSTGETSTTGTTGLTGST
                     SNDTTSSTGSPDTGSTSGAESSSTAGFEASGLDGTAVTGDPAPTSDGSPTSDGTAADT
                     DSSVSETADDPSETATDKGCGCASNDPAPGASLLLLGLLGLRRRRARAQSRV"
     gene            complement(3258..3467)
                     /locus_tag="VGB85_15060"
     CDS             complement(3258..3467)
                     /locus_tag="VGB85_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135401.1"
                     /translation="MLHQRDLDDPHSPRIATELPRFGAYRVEAELTARPTGELPPLQS
                     GASPAYDLAAYSATVTRLGFTLDSR"
     gene            3546..4268
                     /locus_tag="VGB85_15065"
     CDS             3546..4268
                     /locus_tag="VGB85_15065"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0135402.1"
                     /translation="MSRSDARDRLIQATIDLVWTSSYGAVSVDAICERAQVRKGSFYH
                     FFASKDELVVEALDAHWSARRPVLDEIFSPSRPPLERLERYFAYVVERQSAVRAQYGR
                     VLGCFHNAVGSECVQRPSEIAAKAQTIIVTLRRYLETALRDAQAEGLMPPGDPAQDAK
                     AMFAYVQGALAQARVHDDLDILRRLPPTAYALVRLTPPKPAATPAATPAATPAATPAP
                     TPAATPAATPAPATSPAPVPRA"
     gene            4349..>5100
                     /locus_tag="VGB85_15070"
     CDS             4349..>5100
                     /locus_tag="VGB85_15070"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0135403.1"
                     /translation="MWIVRLALLRPYTFIVMAVLLLLSGTWVVRQMPTDVLPEVDIPV
                     ISVVWTYTGLPAQQMEQQVTLFSEYSLSGNVSDIANIQSDSFDGVSVIRLYLQPDADV
                     AAAMAQVTAASQTIVRRMPPGIVPPLILRYSASSVPIIQIAFSSDTLSESEIFDHVNQ
                     RVRTMLSVVRGTRFPLPSGGKARQIAVDLDPAALRAHGVSPADVSAAISAQNLTLPTG
                     SAKIGEREYRISLNSSPEAIAALNEVPLKSRAG"
BASE COUNT          713 a         1728 c         1846 g          813 t
ORIGIN      
        1 cccggtgctc ccgccgtcat caccacatgc agcgctcacc gccagccacc cgcagatcca
       61 cccgaccgct cgttgcttca ccatcctcca ccccccggtc gcatggtacc cccggccccc
      121 gggcggcgcg tgggagatgc gctggcgcac ccgctcgcgg acgagccgtg cgcgcgcgag
      181 cccgctgtgt cgaggtttgc gcggggccgg gggccaacgc gctcgtggcg accgttgaca
      241 ccgcgtcgac gaaggacttc actgcgaccc gatgcaggag agcacctcga cggcacccgc
      301 cgagctggcg aagctctcct cgccggcgat cgtcgcgcgc gtcgtcgcgg agccgtcgct
      361 ggtcaagctc gcgggcgcga tcttcgagtc gcgcgcgacg cacccgcgag cggccgagat
      421 gctgtccgag gcgtacgcgt cgggcaaggc gccgccgtgg atgaccgcat atctgctcgg
      481 ctgcatgcgc gcgaccgcga tgtactcggt ggtccgcgcg atcctcgtca gcgcgccggg
      541 gctgctcgcc gagagctacg ccggcgtggc gctggtgaag atctccggcg acgcggcgcg
      601 tgaggacctg ctcgcgctgc tcgccggacc ctacccgcgc gcgtgccgtg acggggcgat
      661 gtacggcctc gcggccctgc gcgacccctc actcgtggcg ccgctgctga cgctggcccg
      721 caccggccag atcgcgagca aggccgcggc gcacatcatc gcggccgata acccgacccc
      781 cgagcagctg gtcggctggc tcggctcacc ggaggcgagc gaccgctcgg tggcgctacg
      841 ggtgatcgtc ggcgcggcca agatcgaccg cgaacgcaag ttcgacccga ccgtcgtgca
      901 ggcgacccgc caggcgctcg ggcaggcctc cgacttgccg gagtacctga gcacctggct
      961 gcgccagcgc ctcagcggat tctgaggcgc ccgggttaca cccggctttg ggcgcgggcg
     1021 cgacggcgac gcaggccgag taggccgagc agcaacagcg acgcgcccgg ggccgggtcg
     1081 ttgctggcgc atccgcagcc cttgtcggtc gcggtctcgg acgggtcatc ggcggtttcg
     1141 ctgacgctgc tgtcggtgtc ggccgcggtg ccgtcgctgg tcggcgagcc gtcgctggtc
     1201 ggggcggggt cgccggtgac ggcggtgccg tcgaggccgc tcgcctcgaa gcccgcggtc
     1261 gaggagctct cggcgccgct ggtcgagccg gtgtcgggcg agccggtcga gctggtcgtg
     1321 tcgttcgagg tcgagccggt caagccggtc gtgccggtcg tgctggtctc gcccgtgctc
     1381 gtgctcgtgt cgttcgggcc ggcggtgctg ccaccgttct cacccgtgct aaacgagtcg
     1441 tccgtgtcgc tgaggtcgcc ggtctcgccc atcggctcgc cgccgccgtc gcactgctgg
     1501 aacgcgcagc actcgtcgaa gaccgggagc acgaggaagc cctggaacgc ggcctcgagc
     1561 tcgtcctggg tgttggcgat cgtcggcggg agggtcgcgc cggagcccca ctcggcgagc
     1621 ttgacgagct gcacggggtc gacgttcgcg ccgtagccga tgacgtgagt gacgacgccg
     1681 gcctcgtgca tggcgagcag gggggcctgg acctgggcgt cggtcgagtc gtatgcgccg
     1741 tcggtgatca ggaagttgag gaacggggtg tccgcgttac agggcatcgg cgcctggtcc
     1801 atcacgcacg cgctgcggta cgcctcgagg ttgctggcga cgagctggag gccgaggtgc
     1861 agggtgttgc ccgaaccatc gcatgcaccc ttgggctggt tcccgttgca ttcgggcatg
     1921 tggctgatcg tcggatcgtc gaagccgggc gggtcgatct gcgagccgtc cttgaaggtc
     1981 cacatgaggg tgggcttcgc gtaggggtcg gtgcagccgg gggcctcgca ggaggtgaac
     2041 gggtcgaggg cccacgcgaa gttgtcctgg tggcaccggc catacgggat catgaccttc
     2101 tcgtcgcccg ggctgccgaa caccgaaagg ccgaagaaga acatgtcctc ggcggggtgg
     2161 ttgttgggga ggacgagctc gaagacgcct ccctcgccgg cgagcccctc gcgcacgcgg
     2221 tcccacgtgc cgacgccggg ggcagcacgc atgtccccgt cgttgagcgt cgaggacgag
     2281 gcgtcgagca ggaccatcac gcgcgggatg cccggcgtgc agatcggcgc gatgaggctc
     2341 tgcttgatct tgatcaggat ggcgttgagg gcgtcgacga tcagctgggt cgcctgggga
     2401 tcggcggcgt cgacggcgtg gtcggtcccg ccggcgaacg cgagcgcgtc agccgtggcc
     2461 ttgtcgccgg ggccgccgaa cgcgacgacg taggtcggga tcgcgaggtt gtcccacatg
     2521 tccttggcga cgagcgtcgg gtcctgggac ggcggggcgt tgtcgagggt gttggtggcg
     2581 ttggtccacg cgccggtggt caggaccatc agcacggccc agcgcttgct ggtgtcgtcg
     2641 gggtggtcgg cgttggcctg ggcgatgtag ttcttggtga aggtgagcga gccgtgggtc
     2701 cacgcgccga tgcccgtgtt ggcgccgccg agcggggcgg gcagggcctc gagcgcggcg
     2761 acgatcgcgt cgccgttggt gcactcggtg tacgccttca ttgggtcatt cgcgtcgtac
     2821 caggggacgt cgagcttgac gccgtcgacc aggcccgagg tatcaccgac gatcttcgtg
     2881 ccgggcacgt tcgggttggg gtcgtggccg aagcggacca ggccgaggat gtagttctcg
     2941 gcgatgaagc cgttgtcgaa gttgacgacg gtaccgacca tctcgatcgc cgcctcccac
     3001 cgcgtgctgt cggcgtcgaa ggcctggttc atcgccgtcg agtcgtcgag cacgaacgcg
     3061 agccgcggtt ttttgaaggt gcagttgccc tccttctggc aatccatcgg cgcagcgccg
     3121 acctgcgggg gctcgacgcc cccgacgaac acgagggcag aaccaaccaa aaggccaagg
     3181 gcgagaggta aacgcttcat ctcgccagtt atccacaatc gcagacgctt gggaagcgcg
     3241 aatcgtgggt cggcctgtca gcggctgtcg agggtgaagc ccaggcgcgt gacggtggcg
     3301 ctgtacgcgg cgaggtcgta ggccggcgag gcgccggact ggagcggcgg gagctcgccg
     3361 gtcggtcggg ccgtcagctc ggcctcgacg cgataggccc cgaagcgcgg gagctcggtc
     3421 gcgattcgcg ggctgtgcgg gtcgtccagg tcgcgttgat ggagcatgac gcggaacctg
     3481 gactggcgaa catcgcccga taccgggccc ttgcgtggag acgatcggtc aagtaagaca
     3541 gaggggtgag ccgaagcgac gcccgggacc gcctgatcca ggccaccatc gacctggtct
     3601 ggacttccag ctacggggcg gtcagcgtcg acgcgatctg cgagcgcgcg caagtgcgca
     3661 agggcagctt ctaccacttc ttcgccagca aggacgagct ggtggtcgag gcgctcgacg
     3721 cccactggag cgcgaggcgg ccggtgctcg acgagatctt ctcgccctcg cgcccgccgc
     3781 tcgagcgact cgagcgctac ttcgcctacg tggtcgagcg ccagagcgcc gtgagggccc
     3841 agtacgggcg cgtgctcggc tgctttcaca acgcggtcgg cagcgagtgc gtgcagcggc
     3901 ccagcgagat cgccgccaag gcgcagacga tcatcgtcac cctgcggcgt tacctcgaga
     3961 cggccctgcg cgatgcgcag gccgagggcc tgatgccccc cggggacccg gcccaggacg
     4021 ccaaggccat gttcgcgtac gtgcagggcg cgctggccca ggcccgcgtg cacgacgacc
     4081 tcgacatcct gcgccggctc ccgccgaccg cctacgcgct ggtccggctg acgccgccga
     4141 agcctgccgc caccccggca gccaccccgg ccgctacccc ggccgctacc ccggcgccca
     4201 ccccggcagc caccccggcc gctaccccgg cgcccgccac ttcacccgct cccgtcccgc
     4261 gcgcctgatg cccgctgcat cgcgcctgcg ggctcgtttg cgcgcttttc cgacttgact
     4321 aaacagtcat ctcaagagcc ctccaagcat gtggatcgtc cgacttgccc tgcttcgtcc
     4381 gtacaccttc atcgtaatgg cggtgttgct gttgttgtcg gggacctggg tggtccggca
     4441 gatgccgacc gacgttttac ccgaggtcga catcccggtg atcagcgtgg tgtggacata
     4501 caccgggctg ccggcgcagc agatggagca gcaggtcacg ctgtttagcg agtactcgct
     4561 gtcgggcaac gtcagcgaca tcgccaatat tcagagcgac agcttcgacg gcgtgtcggt
     4621 gatccggctg tatttgcagc cggacgcgga cgtcgccgcc gcgatggcgc aggtgaccgc
     4681 ggcctcgcag acgatcgtgc gccgcatgcc gccgggcatc gtgccgccgc tgatcctgcg
     4741 ctactcggcc tcgagcgtgc cgatcattca aatcgccttc tcgagcgaca ccttgtcgga
     4801 gtccgagatc ttcgatcacg tcaatcaacg cgtgcgcacg atgctctccg tggtccgcgg
     4861 gacccgcttc ccgctgccca gcggcggcaa ggcgcgccag atcgcggtcg acctcgaccc
     4921 ggcggcgttg cgggcccacg gggtgtcgcc ggcggacgtc agcgcggcga tctcggcgca
     4981 gaacctgacg ctgccgactg gttcggccaa gatcggcgaa cgcgagtatc gcatcagcct
     5041 caacagcagc ccggaggcga tcgctgcgct caacgaggtg ccgctgaagt ctcgcgccgg
//
LOCUS       DASWNJ010000889         1799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_475057, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000889 DASWNJ010000000
VERSION     DASWNJ010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475057"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VGB85_15075"
     CDS             <1..463
                     /locus_tag="VGB85_15075"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEY0135404.1"
                     /translation="QAYWEALGLPADTLVFENGSGFSQRGRVTTTALVDLIAVASRVS
                     GGQGLLSALPVAGEEGTLRARLRRSGKRVRAKTGTMAGISGLTGVITAEDGTPQVAFS
                     ILSNVRDGGAGAATRRKRAEDLIVTAVLKALDEWALERSTTGAGAAAGTAP"
     gene            493..1470
                     /locus_tag="VGB85_15080"
     CDS             493..1470
                     /locus_tag="VGB85_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135405.1"
                     /translation="MTRALAGSSLVAAVALLGCFRGGFLDDTCEQRGGCDDSTTGATT
                     TPTTSTGDASSGTSTGEPPDTTTGSPGLLFPGPAFRISALEIVDPPLFTRLGDACIDA
                     SSFVNGGLMASVETYDTNLILLAADYDPAQGTQNYQFYSSASCPPGEGYCVINPALAP
                     TPFIAANKDEGSCGDVDATTINPTNLGFLHTPMAPCVVSPTASIMLKLSKDLPSIGFL
                     LGKFAAEYRPDPEAPERLDNATLQGFITEADALNINYTFMNKPVNLWSVIRGSGHPEA
                     CPIPPDMDPGSVSDVDMVDVDGAGPGEPIRGVFLYMNFTAKKIDLYAPL"
     gene            1513..>1799
                     /locus_tag="VGB85_15085"
     CDS             1513..>1799
                     /locus_tag="VGB85_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135406.1"
                     /translation="MALSPVDRRLTTLERLSAIEATDLRGALQQASQLIAEALDTDKV
                     DTFMLDPTVATLVALGTSDTPLGRKQKALGLDRMPLANGGSAPRRSVASIAL"
BASE COUNT          283 a          645 c          627 g          244 t
ORIGIN      
        1 gcaggcgtac tgggaggcgc tcggcctgcc ggcggacacg ctggtgttcg agaacggctc
       61 gggcttcagc cagcgcgggc gcgtgacgac gaccgcgctg gtcgacctga tcgcggtggc
      121 gagtcgggtc agcggcgggc agggcctgct gtcggcgctg ccggtcgccg gcgaggaggg
      181 cacgctgcgg gcccggctgc ggcgctcggg caagcgggtg cgcgccaaga ccggcacgat
      241 ggccggcatc agcggactga cgggtgtgat cacagcggag gacggcacgc cgcaggtggc
      301 cttctcgatc ctcagcaacg tgcgtgacgg cggagcgggc gccgcaacac gtcgcaagcg
      361 ggccgaggat ctgatcgtca ccgcggtgct caaggccctc gacgagtggg cgctcgagcg
      421 ctcgacgacc ggggccgggg ccgcggccgg gaccgcgccc tgacgcgccg cacgatcctg
      481 tatgctgcga cgatgacgcg cgcgctggcc ggctcatcgc tcgtggccgc cgtggccctg
      541 ctgggctgct ttcgcggcgg tttcctcgac gacacctgcg agcagcgcgg cggctgcgac
      601 gacagcacga ccggcgcgac gacgacaccg accacgagca cgggtgacgc gagcagcggc
      661 acgagcacgg gcgagccgcc ggacacgacg acgggctctc cgggcctgtt gttcccgggc
      721 cccgcgtttc gcatctcggc gctggagatc gtcgatccgc cgctttttac caggctcggg
      781 gatgcttgca tcgacgcgag cagcttcgtc aacggcggtc tgatggcctc ggtcgagacc
      841 tacgacacca acctgatcct gctcgccgcc gactacgacc cggcgcaggg gacccagaac
      901 taccagttct attcgagcgc gagctgcccg ccgggcgagg gctactgcgt gatcaacccg
      961 gcgctcgcgc cgacgccctt catcgccgcc aacaaggacg agggcagctg cggtgatgtc
     1021 gacgcgacga cgatcaaccc gaccaacctc ggatttttgc acacgccgat ggcgccgtgc
     1081 gtggtcagcc cgaccgcgag catcatgctc aagctgagca aggacctgcc ctcgatcggg
     1141 ttcttgctcg gcaaattcgc cgccgagtac cgtccggacc ccgaggcccc ggagcgcctc
     1201 gacaacgcga ccctgcaggg cttcatcacc gaggccgacg cgctgaacat caactacacg
     1261 ttcatgaaca agccggtgaa cctgtggtcg gtgatccgcg gcagcggtca ccccgaagcc
     1321 tgcccgatcc cgccggacat ggacccgggc agcgtcagcg acgtcgatat ggtcgacgtc
     1381 gacggcgccg ggcccggcga gccgatccgc ggtgtgttcc tgtacatgaa cttcaccgcc
     1441 aagaagatcg acctctacgc gccgctgtag cgagcgctgg cgcggcggac ccggctcggg
     1501 caaggtgggc ggatggctct gtcccccgtc gaccgccgcc tgacgaccct cgagcgcctc
     1561 agcgcgatcg aggcgaccga cctgcgcggc gcgctgcaac aggcctcgca gctgatcgcc
     1621 gaggcgctcg acaccgacaa ggtcgacacc ttcatgctcg acccgacggt cgccaccctc
     1681 gtcgcgctcg gcaccagcga cacgccgctc gggcgcaagc agaaggcgct cggcctcgac
     1741 cgcatgccgc tggccaacgg cggcagcgcg ccgcgcaggt cggtcgcctc gatcgcgct
//
LOCUS       DASWNJ010000890         4461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_686775, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000890 DASWNJ010000000
VERSION     DASWNJ010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686775"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VGB85_15090"
     CDS             complement(<1..475)
                     /locus_tag="VGB85_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY0135407.1"
                     /translation="MLCSCGAAPQAPAKPAVKAAVADVGYELRRLRPRDEEKLEAMFD
                     RLRVQAVGEGKRVAVLFSADWCEPCRKLETELGNRHPAEQIGDVRIFELKEEDWKAAT
                     RMDEFDALRRRWSGDAGSYPYLVLLDEKGGKREEMKEAIARLETAGVEPTLAQWFA"
     gene            complement(523..1557)
                     /locus_tag="VGB85_15095"
     CDS             complement(523..1557)
                     /locus_tag="VGB85_15095"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I asparaginase"
                     /protein_id="HEY0135408.1"
                     /translation="MSIRPRVCVLHVGGTIGMIRTATGFEPQPGFLQRYMAAMPELAR
                     LELPAHDVVSLEPLLDSADMAPDDWVRIAAEIAARYDDYAGFVVVHGTDTMAYTASAL
                     SFLLPNLGKPVILTGSQLSLENVRSDGREHIITSILLAGTLPVPEVCIYFASRLLRGN
                     RAQKIHNLDFVAFDSGNLRPLATVGASIELNVHLVRPSGNGKVVAPTLTNRPEVAALR
                     LFPGIGVRMLEHLLAAPTQGLVLETYGVGNAPSRDPEFLAAISAATAAPREVVVVNCS
                     QCHGGAVIQTLYSTGAALARCGVISGNDMTPEAALTKLYCLLAAGLRPDEVRARMQEN
                     LAGELDPGDS"
     gene            complement(1752..2801)
                     /locus_tag="VGB85_15100"
     CDS             complement(1752..2801)
                     /locus_tag="VGB85_15100"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEY0135409.1"
                     /translation="MNRSSTSEAKQDSSRTVQETARLILRHLRDNADGASKEALAAKA
                     GVAGVTVQRALKYMREEWDAPLEFVHGSKLWRLLDNSFTLPLTDPEPEDLAAVVFAAA
                     LLSPVADAELNARVKRLVEQMDAAVEEAGKSKTEPQGKVRPRSVTATVTTGTRMDSRL
                     VGRLLQAVGTGVVRIEYDSPWSGTRKTHDIEPWQLRVHDGVMYVRAWSRTTGDARVFR
                     VAQIVHARVLPGQAPREPAPPKGNIWGTSDPGYGIDKDRPGVATLRVRGGVARWIANM
                     VWDPGQEDRWIEAGELLERRVPYHSCRELARRLLSIVDGLESIDPPELRDEVVTDARA
                     FLQRIDEMAAGPAPV"
     gene            3153..>4461
                     /locus_tag="VGB85_15105"
     CDS             3153..>4461
                     /locus_tag="VGB85_15105"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEY0135410.1"
                     /translation="MRRDLAGATVLRVITVVHAADLHIDSPLRGLGRLEGAPVDDLRL
                     ATRRAFVKLIDTCLEEQAALLILAGDVFDGEWRDFNTGLFFVRELARLRDTGTQVVMV
                     RGNHDAESVLARHIPLPEHVFTFSCDAPESRDYSRLGLAVHGQSYGARSVEDNLADAY
                     PAHRRDLFNIGVLHTNAVASSEHGNYAPCTVAQLVRHGYDYWALGHVHQAQILNRDPW
                     VVYPGNLQGRHARETGAKGCTILRLDGLRVDRVESREVDVLRWVDLVVSADDIHGSSD
                     MDDLLGEIRSRMQQSITEAGGRHVLARVTVEGSTPLHTALAADPGAVQAQVRALAAEL
                     GDIWPEKIRFVTRPPLGELGDLHDLFQALRDELAGLAADPEALGRYSEGLAPLVTAAA
                     PFLGHRPDDPERLVTRLGELEALLLARLGGGHVGASVPKASSAE"
BASE COUNT          622 a         1789 c         1381 g          669 t
ORIGIN      
        1 cggcgaacca ctgcgccagc gtcggctcga cgcccgccgt ctcgaggcga gcgatcgcct
       61 ccttcatctc ctcgcgcttg ccgcccttct cgtcgagcag caccagatac ggataggacc
      121 cggcgtcccc gctccagcgc cggcgcagcg cgtcgaactc gtccatgcgc gtcgccgcct
      181 tccagtcctc ctccttgagc tcgaagatcc ggacgtcgcc gatctgctcg gccgggtggc
      241 ggttcccgag ctccgtctcg agcttgcgac acggctcgca ccagtcggcg ctaaacagca
      301 ccgcgacccg cttgccctcg ccgaccgcct gcacgcgcag ccggtcgaac atcgcctcga
      361 gcttctcctc gtcgcgcggc cgcagccgcc gcagctcgta gccgacatcg gcgaccgccg
      421 ccttcacggc cggcttcgcc ggagcctgcg gcgccgcccc gcagctgcac agcatcagcg
      481 cgacaaacca cgccagtgac ctgtctcttc gtacatgtcc catcagctgt cccccggatc
      541 gagctcgcct gccaggttct cctgcatgcg cgcgcgcacc tcgtccgggc gcagccccgc
      601 ggcgagcagg cagtacagct tggtcagcgc cgcctcgggc gtcatgtcgt tgcccgagat
      661 caccccgcag cgggccagcg ccgcgccggt gctgtacagc gtctggatca ccgcgccgcc
      721 gtggcactgc gagcagttga cgaccaccac ctcgcgcggc gccgccgtgg ccgcggagat
      781 cgccgcgagg aactccgggt cgcggctcgg cgcgttgccg accccgtagg tctcgagcac
      841 cagcccctgc gtcggcgcgg ccagcaggtg ctcgagcatc cgcaccccga tgcccgggaa
      901 cagccgcagc gccgcgacct cgggccggtt ggtcagcgtc ggcgcgacca ccttgccatt
      961 gcccgacggc cgcaccaggt gcacgttgag ctcgatgctc gcgccgacgg tcgccagcgg
     1021 ccgcaggttg ccgctgtcga acgcgacaaa atcgaggttg tggatcttct gggcccggtt
     1081 gccccggagc agccgcgagg cgaagtaaat gcacacctcc ggcaccggca gcgtgcccgc
     1141 gagcaggatg ctggtgatga tgtgctcgcg gccgtcgctg cgcacgttct cgaggctcag
     1201 ctgcgagccg gtcaagatca ccggcttgcc gaggttcggc agcaagaagc tcagcgccga
     1261 ggccgtgtag gccatcgtgt cggtcccgtg cacgaccacg aagccggcgt agtcgtcgta
     1321 gcgcgcggcg atctcggcgg cgatgcggac ccagtcgtcg ggcgccatgt cggccgagtc
     1381 gagcaggggc tcgaggctga cgacgtcgtg cgccggcagc tcgaggcgcg ccagctccgg
     1441 catcgccgcc atgtatctct gtaagaaccc cggctgcggc tcgaagccgg tcgccgtccg
     1501 gatcatgccg atggtccccc caacgtgcag gacacagacg cgtgggcgga tgctcatggc
     1561 cggcgcacta tgccgagccc tccgcgcgcc tgcaacaccg acaaggctgt gggggcgagc
     1621 tcacgcccgc ccccacaggg gttgaggtca acactaccta ccccaccaag gccaacaccc
     1681 acggctgacc tcccgggcac tgtgcctgac ctgccttgtc tggcggagat caagccgcaa
     1741 gtactgtctc ttcagacagg cgcgggaccg gccgccatct cgtcgatgcg ctgcaggaac
     1801 gcccgcgcgt cggtgaccac ctcgtcacgc agctcgggcg ggtcgatcga ctcgagcccg
     1861 tcgacgatgc tcaacaatcg ccgcgccagc tcgcgacacg agtgatacgg cacgcgccgc
     1921 tcgagcagct cgcccgcctc gatccagcgg tcctcctgcc ccgggtccca caccatgttg
     1981 gcgatccatc gcgccacgcc gccgcgcacg cgcagcgtcg ccacgcccgg ccggtccttg
     2041 tcgatcccgt agcccgggtc cgacgtgccc cagatgttgc ccttgggcgg cgccggctcg
     2101 cgcggggcct gccccggcaa cacccgcgcg tgaacgatct gcgcgacgcg aaacacccgc
     2161 gcgtcgcccg tcgtccgcga ccacgcgcgt acgtacatca ccccgtcgtg cacccgcagc
     2221 tgccacggct cgatgtcatg cgtcttgcgc gtgccgctcc acgggctgtc gtactcgatg
     2281 cgcaccaccc ccgtgccgac cgcctgcaac agccgaccga ccagccgcga gtccatcctc
     2341 gtccccgtcg tcaccgtcgc cgtcacgctg cgcgggcgca ccttgccctg cggctccgtc
     2401 ttgctcttcc ccgcctcctc gaccgccgcg tccatctgct cgaccagccg cttgacgcgg
     2461 gcgttgagct ccgcatccgc gaccggcgac agcagcgccg ccgcgaacac caccgccgcg
     2521 aggtcttccg gctccgggtc cgtcagcggc agcgtgaagc tgttgtcgag cagccgccag
     2581 agcttgctgc cgtgcacgaa ctcgagcgga gcgtcccact cctcgcgcat gtacttgagg
     2641 gcgcgctgca ccgtcacgcc cgccaccccg gccttggcgg ccagcgcttc cttcgaggcc
     2701 ccgtcggcgt tgtcccgcag gtgccgcaag atgaggcgcg ccgtctcctg cacggtcctg
     2761 gacgagtctt gcttcgcttc gctggtgctg ctccggttca cagtgggctc ctgggggcgc
     2821 ccatcctacc gcctgcgggg cgtcggacgg gcctcgataa gtgacgaatc gcgcgcccgg
     2881 gcacctttgc ccgcgcgcgc gcaccttcga cgtaagcatt cagatcgcca atcaacgcgc
     2941 tttggcgggt gatctgctcc agagcatgcg ggcgtcgggc gagcacaaat cccctcgccc
     3001 ccgcgcgaaa tagagcgccc cggaagcctc gccgccccct cgcttctaca ccgcacgatc
     3061 gcctcgtgcc ccgcgcgcac gaccggcgat ctcggcgcca gcgagatccg cgtcaatcca
     3121 gcgatctcgc ggccctgcac gctgtttgcg gcttgcgccg tgatctcgcg ggtgccactg
     3181 tccttcgcgt gatcacggtc gtccacgctg ccgatctgca catcgacagc cccctgcgag
     3241 gcctcggtcg cctcgagggc gcaccggtgg acgacctgcg cctcgccacc cgccgcgcct
     3301 tcgtcaagct gatcgacacc tgcctcgagg agcaggccgc cctgctcatc ctcgccggcg
     3361 acgtcttcga cggcgagtgg cgtgacttca acaccggcct gttcttcgtc cgcgagctcg
     3421 cccgcctgcg tgacaccggc acccaggtcg tcatggtccg cggcaaccac gacgccgagt
     3481 ccgtgctcgc tcgccacatc ccgctgcccg agcacgtctt caccttctca tgcgacgccc
     3541 ccgagtcgcg cgactacagc cgcctcggcc tcgccgtgca cggtcagagc tacggcgccc
     3601 gctcggtcga ggacaacctc gccgacgcct accccgccca ccgccgcgac ctgttcaaca
     3661 tcggcgtgct gcacaccaac gccgtcgcca gcagcgagca cggcaactac gccccctgca
     3721 ccgtcgccca gctggtccgc cacggctacg actactgggc gctcggccac gtgcaccagg
     3781 cccagattct taatagggac ccatgggtcg tgtaccccgg caacctccag ggccgccacg
     3841 cccgcgagac cggcgccaag ggctgcacga tccttcgcct cgatggcctg cgcgtcgacc
     3901 gggtcgagtc gcgcgaggtc gacgtgctgc gctgggtcga cctcgtcgtc tccgccgacg
     3961 acatccacgg gtcctccgac atggacgacc tgctcggcga gatccgctcg cgcatgcaac
     4021 agtcgatcac cgaggccggc ggccgccacg tgctcgcgcg cgtcaccgtc gagggcagca
     4081 cgcccctgca caccgccctc gcggccgacc ccggcgccgt gcaggcccag gtgcgggccc
     4141 tcgccgccga gctcggcgac atctggccag agaaaattcg cttcgtcact cgccccccgc
     4201 tcggcgagct cggtgacctc cacgaccttt ttcaagccct gcgcgacgag ctcgccggcc
     4261 tcgccgccga ccccgaggcg ctcggccgct acagcgaggg cctcgccccg ctcgtcaccg
     4321 ccgccgcgcc cttcctcggc caccgccccg acgaccccga gcgtctggtg acacgcctcg
     4381 gcgagctcga ggccctgctg ctcgcccgcc tcggcggcgg ccatgtcggt gcatctgtcc
     4441 cgaaggccag ctccgcggag c
//
LOCUS       DASWNJ010000891         3167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_16676, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000891 DASWNJ010000000
VERSION     DASWNJ010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16676"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VGB85_15110"
     CDS             <1..373
                     /locus_tag="VGB85_15110"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0135411.1"
                     /translation="GVYLDPELTRLCGMAAPKAFFEFDSTVVEGADNSGLSSLAQCVS
                     TGPLKGRKLELVGHADPRGTDAYNDQLGKSRAESVMDFLSTEGVPADRMTTRSEGERG
                     ADVTNADEWPYDRRVDIRLVP"
     gene            481..1770
                     /locus_tag="VGB85_15115"
     CDS             481..1770
                     /locus_tag="VGB85_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135412.1"
                     /translation="MHRKLTTALLLALPACVLDKDLGNGTLLTTDSTGEPGATDDGGA
                     TGDGTEPTTGVPNDCPEGQPHLAQLWQFEYDQFLDFQSSFAPIGRLADGRIAVPGNFD
                     LAPDQHGVALMLVAPDGQLLGPQTAAPGDKRVGAVAFAIGADDQPVVLGNHGSDIDGV
                     NIMKLTRFTSDMQLVSQIDVPFVEDRGPPYVPTLALSADGPVVAGPVPGGPEPVLVKL
                     APDTGALVWQHTFTATDDLVITEIAIGPTGDIAVAGHADTELDAQDAVRLWRFDPAGA
                     QIWERVITVPEFEEVTALHFAPDGQIVALRNGKEFAASVDLVSVEVATGETRWALTVA
                     AVEDELGGWAEDMHVDADRFTIPMSRSRVHHHAPADTNWTEVRTVSFAGELLAVTKVP
                     GVLGDGGLSWLRSVRGRCGELILVRDIDSLQVTALAP"
     gene            complement(1801..>3167)
                     /locus_tag="VGB85_15120"
     CDS             complement(1801..>3167)
                     /locus_tag="VGB85_15120"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135413.1"
                     /translation="VTRLQAEIRSLTQRIDAGIRERDKLQQSLGTRREDGLRLTAELA
                     EIRSKLPELEQQGHDAESRLETLREQQARVAEMLTEARVALARFQQQRDALAQARARL
                     ERQVTGERDRLQRLTQSFEEGEARSAQLVSEAEAAAALRETLLVEHRAATELLQSSRE
                     AHDAARLAQSEVDLAVRNLRGSFDLQREQLGEVELGLKELRLELTHLTQDIRERQDAE
                     LALVLADHHDRPIAGPEEVERQKELKRVLARMGEVNLTAIEEFEEVSGRVDYLSTQKN
                     DLETAIAQLQEAIDKINKTTRERFVETFKSVNDMFQQVFPRLFSGGKAELKLTDPSDM
                     LGTGVEIVAQPPGKQVRSLELLSGGEKALTAVSLIFAIFLIKPSPFCLLDEVDAPLDE
                     ANVGRFGAMVAEMSENTQFIIITHNKRTMEIADRLYGVTMEVKGVSKLVSVNIRRAVE
                     MAVA"
BASE COUNT          436 a         1144 c         1102 g          485 t
ORIGIN      
        1 cggcgtctac ctcgaccccg agctgacccg cctgtgcggc atggctgccc ccaaggcctt
       61 cttcgagttc gactcgaccg tcgtcgaggg cgccgacaac agcggcctga gctcgctcgc
      121 gcagtgcgtc agcaccggcc cgctcaaggg tcgcaagctc gagctcgtcg gccacgccga
      181 cccgcgcggc accgacgcgt acaacgatca gctcggcaag tcgcgcgccg agtcggtgat
      241 ggacttcctg agcaccgagg gcgtgccggc cgaccgcatg acgacccgct cggagggcga
      301 gcgcggggcc gacgtcacca acgccgacga gtggccctac gaccgccgcg tcgacatccg
      361 cctggtgccc tgagccgcct caccgggccg cgcgggcggg cgcggtcgcg ctcggtcgcc
      421 atcgcgaatt tcgcgtggcc ccggtgaaag atcggcgggc agccggggac gaccagggat
      481 atgcaccgca aactgaccac cgcgctcctg ctggccctcc ccgcctgcgt cctcgacaag
      541 gacctcggca atggcacgct cctgaccacc gactcgacgg gtgaacccgg cgcgaccgac
      601 gacggcggcg cgaccggcga tggaaccgag ccgacgaccg gcgtgccgaa cgactgtccc
      661 gaaggacagc ctcacctcgc gcagctgtgg cagttcgagt acgaccagtt cctcgacttc
      721 cagagcagct tcgcgccgat cgggcggctg gcggacgggc ggatcgccgt cccgggcaac
      781 ttcgacctcg ccccggacca gcacggcgtg gcgctgatgc tggtggcacc ggacggtcag
      841 ctcctcggcc cccagacggc ggcgcccggc gacaaacgcg tcggcgccgt cgccttcgcc
      901 atcggcgcgg acgaccagcc ggtcgtgctc ggcaaccacg ggagcgacat cgacggcgtg
      961 aacatcatga agctgacgcg cttcacctcc gacatgcagc tcgtctcgca gatcgacgtg
     1021 ccgttcgtgg aggaccgggg cccgccatac gtgccgacgc tggcgctgag cgccgacggg
     1081 ccggtcgtcg cggggcccgt ccccgggggg cccgagcccg tcctggtgaa gctcgccccg
     1141 gacaccggcg cgctcgtatg gcagcacacg ttcaccgcca cggacgacct ggtgatcacc
     1201 gagatcgcga tcgggccgac gggcgacatc gcggtcgcgg gtcacgcgga tacggagctc
     1261 gacgcacagg acgcggtacg cctgtggcgc ttcgaccccg ccggcgcgca gatctgggag
     1321 cgcgtgatca ccgtgccgga gttcgaggag gtgaccgccc tgcacttcgc gccggacggc
     1381 cagatcgtcg cgctgcgcaa cggcaaggag ttcgcggcgt ccgtcgacct ggtgtccgtc
     1441 gaggtcgcca ccggcgagac gcgctgggcg ctgacggtgg cggcggtgga ggacgagctc
     1501 ggcggatggg ccgaggacat gcacgtcgac gccgaccgct tcacgatccc gatgtcacgc
     1561 agccgggtgc accaccacgc cccggcggac accaactgga ccgaggtgcg caccgtgtcg
     1621 ttcgcgggcg agctgctcgc cgtcacgaag gtgccgggcg tactcggcga cggcgggctc
     1681 tcgtggctcc gcagcgtgcg cggccgctgc ggcgagctga tcctggtccg cgacatcgac
     1741 tccctgcagg tcaccgcgct cgcgccgtga gctcgcccgc ccacaccggg ggtctgtgcg
     1801 ctacgcgacg gccatctcga ccgcgcggcg gatgttgacg ctgacgagct tcgacacgcc
     1861 cttgacctcc atcgtcaccc cgtagagtcg gtccgcgatc tccatcgtgc gcttgttgtg
     1921 ggtgatgatg atgaactggg tgttctcgga catctcggcc accatcgcgc cgaagcggcc
     1981 gacgttggcc tcgtcgagcg gggcgtcgac ctcgtcgagc agacagaacg ggctcggctt
     2041 gatcaggaag atcgcaaaga tcaggctgac ggccgtcagc gccttctcgc cgccggacag
     2101 cagctcgagg ctgcggacct gcttgccggg cggctgggcg acgatctcga cgccggtgcc
     2161 gagcatgtcg ctcgggtcgg tcagcttgag ctccgccttg ccgccgctga acaggcgcgg
     2221 gaacacctgc tggaacatgt cgttgacgct tttaaaggtc tcgacgaagc gctcgcgcgt
     2281 cgtcttgttg atcttgtcga tcgcctcctg gagctgggcg atcgccgtct cgaggtcgtt
     2341 cttctgcgtc gacaggtaat cgacgcggcc cgagacctct tcaaattcct cgatcgccgt
     2401 caggttgacc tcgcccatgc gcgcgagcac ccgcttgagc tccttctgac gctcgacctc
     2461 ctcgggtccg gcgatcgggc ggtcgtggtg gtcggcgagc acgagcgcca gctcggcgtc
     2521 ctggcgctcg cggatgtcct gcgtcaggtg cgtcagctcg aggcgcagct ccttcaagcc
     2581 gagctcgacc tcgccgagct gctcgcgctg gaggtcgaag ctgccgcgga ggttgcggac
     2641 cgcgaggtcg acctcgctct gggccaaacg cgccgcgtcg tgcgcctcgc gcgagctctg
     2701 cagcaactcc gtcgcggccc ggtgctcgac cagcaaggtc tcgcgcagcg ccgccgcggc
     2761 ctcggcctcg ctgacgagct gcgcgctgcg ggcctcgccc tcctcgaagc tctgggtcag
     2821 gcgttgcagg cggtcgcgct cgccggtcac ctgccgctcc aacctcgcgc gcgcctgggc
     2881 cagcgcgtcg cgctgctgct ggaaccgggc cagcgcgacg cgggcctcgg tcagcatctc
     2941 ggcgacgcgg gcctgctgct cgcgcaaggt ctctagccgg ctctcggcgt cgtggccctg
     3001 ctgctcgagc tcgggcagct tgctccggat ctcggcgagc tcggcggtca gccgcaggcc
     3061 gtcctcgcgg cgggtgccga ggctctgttg cagcttgtcg cgctcgcgga tgccggcgtc
     3121 gatcctctgc gtcaggctgc ggatctcggc ctggaggcgc gtgacct
//
LOCUS       DASWNJ010000892         2079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_509090, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000892 DASWNJ010000000
VERSION     DASWNJ010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509090"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..756)
                     /locus_tag="VGB85_15125"
     CDS             complement(109..756)
                     /locus_tag="VGB85_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0135414.1"
                     /translation="MHLYDDAVAARLGGRKKLREVREAAPFTSPAFVGLGERYPSERP
                     DQDGLDSERNRRVDLVFLDPDEAVYIEDKDSVETLLYAPESVARREWLPLGFPARADE
                     LVVKVQTEDGQPVPGAYCTLTLPGGDVREATLDAAGIVRFLGLPPVAGIFRLPDFEAF
                     DWDAVADATRPLPPPPVHDDDPDEEPDDANLPPLTAAERRVFDLEDDDDEDDTET"
     gene            1305..1802
                     /locus_tag="VGB85_15130"
     CDS             1305..1802
                     /locus_tag="VGB85_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135415.1"
                     /translation="MSTPADRGAFGPRSLLATLGMFVGLTLVVVLASTIAGVLERAPT
                     RPAPMLAGAAPPDAATLAFFGALAAGVPFEGWVIDRIDGPRAGGLPLVLRGPAAQQVA
                     VELRPADPRSPASPAASETLAIYVIDRNMPPGGLEGVLAFARALRSREATGARLAGLW
                     PLLAG"
     gene            complement(1788..>2079)
                     /locus_tag="VGB85_15135"
     CDS             complement(1788..>2079)
                     /locus_tag="VGB85_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135416.1"
                     /translation="IHGWPLLASRGRTDAELHAAFASILDRFAVCHVLFSAGHNLFAG
                     EFSEPARARAGLERALAERGRVVRERRVFASEAGTPALALWSLSGCGSSTRQ"
BASE COUNT          294 a          718 c          746 g          321 t
ORIGIN      
        1 gtgctgcgtg atcgggtcga gcgagccgaa accgaagtat gcggcaacga cgcgcagatg
       61 ttcgccagac ttgagctgat attgaacgcc ggccgctgct gcgtacattc atgtctccgt
      121 atcatcttcg tcgtcatcgt cttcgagatc gaagaccctg cgctcggccg ccgtgagggg
      181 cggcaagttg gcgtcgtccg gctcctcgtc ggggtcatcg tcgtgcacag gtgggggtgg
      241 taacggacgg gtcgcgtccg cgacggcgtc ccagtcgaag gcctcgaagt cgggcaggcg
      301 gaagatgccc gcgaccggtg ggagcccgag gaagcgaacg atgccggcgg cgtcgagcgt
      361 cgcctcgcgg acgtctccgc ccgggagcgt caaggtgcag tacgcacctg ggacaggctg
      421 accatcctcg gtctggactt tgacgacaag ctcatcggct cgggccggga agccgagggg
      481 tagccactcg cgccgcgcga cgctctcggg cgcgtacagc aaggtctcga cgctgtcctt
      541 gtcctcgatg tagaccgcct cgtccgggtc gaggaacacg agatcgacgc ggcggtttcg
      601 ctcgctgtcg aggccgtcct ggtcgggccg ctcgctgggg taacgctcgc ccaggccgac
      661 gaacgcgggt gacgtgaacg gtgcggcctc ccggacctcg cgtagcttct tcctgccgcc
      721 gagacgggcg gcgacagcgt cgtcgtacag gtgcataaag gccctccagt cctccacacg
      781 ggccgccttg cccgcccatg ccgcgatctt cgcgtgctcc gcgaagtcgt cgcggaagtc
      841 ctgtagggcc gcgcgcgtga gcggtccgaa ctcgccgtcg acagtaccgg ggtggcagtc
      901 gaagtccggg aacgtcgcgc tgacccataa cagtgaatcg cccgtacgcc gactgggtgc
      961 cgttctccgg gtatcgcgcc tccgcctgtc cgacgatccg cacgccgccg aagctggcat
     1021 aggcgctgac gggggtgtcc catggcacca cgagctgctc cccgttcacc accttgccgc
     1081 ggtcgtgcga cacgaaagtt tcgatgtcgc cccccgcgtc gaaggtgagc gccgcctcga
     1141 tcgtgtgccc ggcgttggtg tagcgcgcgc gcagcgtcga gtcgtcgacg accgtccaga
     1201 tcaccggcgc gccgatcaac gacgcggggg cgtcgccggt ccccgggtcg aggacgcgac
     1261 cgacgaccgt ggcgatgaaa gccggggtct ggcaagctgc ccgcatgtcg acgcccgccg
     1321 accgtggtgc gttcggaccg cgaagcttgc tcgccacgct cggcatgttc gtcggtctga
     1381 cgctcgtggt cgtgctcgcc tcgacgatcg cgggcgtgct cgagcgcgcg ccgacgcgtc
     1441 cggcgccgat gctcgccggg gcagcgccac ccgacgcggc gacgctggcg ttcttcgggg
     1501 cgctcgccgc gggcgtgccc ttcgagggct gggtgatcga tcgcatcgat gggccccgcg
     1561 caggcggact gccgctggtc ttgcgcggac ccgcggcgca gcaggtcgcc gtcgagctgc
     1621 ggccagccga cccgcgctcg ccagcgtcgc ccgcggcgag cgagaccctg gcgatatacg
     1681 tgatcgatcg caatatgccg cctggaggac tcgagggcgt gctggctttc gcccgcgcgc
     1741 tgcgctcgcg cgaggccacc ggtgcacggc tcgccgggct ctggccacta ttggcgggtt
     1801 gacgacccgc agccggacag cgaccacagc gcgagggccg gcgtgccggc ctcgctggcg
     1861 aacacccgac gctcacggac cactcgcccg cgttcggcga gcgcacgctc gaggcccgca
     1921 cgggcccgcg cgggctcgct gaattcgccg gcgaagaggt tgtgcccggc gctgaacagt
     1981 acgtggcaga ccgcgaaacg atcgaggatc gatgcgaacg cggcgtgcag ctccgcgtcc
     2041 gtgcggccgc ggctggcgag cagcggccag ccgtggatc
//
LOCUS       DASWNJ010000893        14276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_16690, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000893 DASWNJ010000000
VERSION     DASWNJ010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 14276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14276
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16690"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VGB85_15140"
     CDS             <1..869
                     /locus_tag="VGB85_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135417.1"
                     /translation="ESPVGPMVDVSDPQLYEFSFKASDADPEASLALGAQLAELDTTV
                     APIGKLVVYLHGAGEPETCGSKPHGVVLAKMGFHVLQPCYVSGYGVDKCKDDIGGCRL
                     EAFEGVDHHPVIDITPPNSIETRVVRGLEYLQAMHPGGDWQYFIVDGEPRWSQIIISG
                     ISHGASSSGVIGMVREVDRAVMLSGPLDTDQAWLKGVPLTPIDRFYGFTHTADPQHPG
                     HLKSFGDIMLPGEPVVVDAATPPYDDSHRLVTSAPTGDGHGSTQAGGASPKDGQDAYL
                     FLPVWRTMYGAP"
     gene            complement(880..1524)
                     /locus_tag="VGB85_15145"
     CDS             complement(880..1524)
                     /locus_tag="VGB85_15145"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEY0135418.1"
                     /translation="MPDPRRRLAVLHRQLRACQACPSMHGPSVHGPAIAAEVLLVGQA
                     PGPHEGAIGRPFAWTAGRTLFRWFEAATGVDEAMFRAGVYMSAVARCFPGKATGGGDR
                     RPDREEIARCQPFLARETQILQPRLVLPVGGLAIEQVLGHKGPLVEVVGVQRRVRYHG
                     VDCDVICLPHPSGVSTWHRVEPGIRLLAEALRLIGEHPAFARLRAASAATQLGA"
     gene            complement(1576..1851)
                     /locus_tag="VGB85_15150"
     CDS             complement(1576..1851)
                     /locus_tag="VGB85_15150"
                     /inference="COORDINATES: protein motif:HMM:NF025143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4169 family protein"
                     /protein_id="HEY0135419.1"
                     /translation="MSDRATQKGRWYLQHASDMLAAVLTIMADVINLSRFRKAKARAD
                     KARLAEEQRIRHGRSKIEKQAVASEQHRMADVLAGKWLVRDKEPEPG"
     gene            2090..2596
                     /locus_tag="VGB85_15155"
     CDS             2090..2596
                     /locus_tag="VGB85_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135420.1"
                     /translation="MHRYNFHALTFTCILLGACGGGKNDTDTEASDSTAGATGTDTTA
                     DATDATDATESVGMSSAPTTSGDDGSGSANPTQDSGTATDSTAGGLTTTGDTATTDGD
                     PDLMQLCESWCARNAECQQSDPDGCAADCFADLGSQEGIVHRCDDHDARLHARHDLRA
                     VGRVHRQR"
     gene            2547..2867
                     /locus_tag="VGB85_15160"
     CDS             2547..2867
                     /locus_tag="VGB85_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135421.1"
                     /translation="MLGMTCEQWVAFTDNDEPGPCAAEADASENACADEVCTASIGTS
                     EDNTECSYSVECPNQPSREIQCAGETCTCLEDGEEVGQCPAMDVCMNVDDLTSKAMDC
                     CGFV"
     gene            complement(2873..3454)
                     /locus_tag="VGB85_15165"
     CDS             complement(2873..3454)
                     /locus_tag="VGB85_15165"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY0135422.1"
                     /translation="MSSLSHHFFTMACNNAWANHRLLSACLRLTQAEFAATRTSFFPS
                     IRATLNHNVTVDWYYVDALERALRGEPVNPEARGFFEPAEPFTDCAALLAAQHAVDRR
                     LITACRVSNEDRLALPVAILRRTGVKHETVQRTLAHLFQHQIHHRGQAHAMLAGTAVP
                     PPPLDEFFCAEEAELRAAELAELGLSEAQIWGE"
     gene            3517..4209
                     /locus_tag="VGB85_15170"
     CDS             3517..4209
                     /locus_tag="VGB85_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135423.1"
                     /translation="MTYLRALLPTLLALPLACGDKSSDDTGDASTSSTSSTSSTSSTS
                     STSASASSTTSATDVTTGGTPTTDIGTSTTSTTSDASESGSSEGGSGTGAPVCSLEQV
                     ACDLAESNGEFEDCGSVDPWDDDTAAWQAAHDCAVAAASEQRAFKVVTWLQGIDSQIG
                     VAYASLVGRSYGLSHFGFDSDPCGGGGCGPVVYGGSCDLLVATPDCMVEPGQACLTCV
                     NQGDSTQFCGPE"
     gene            complement(4240..5199)
                     /locus_tag="VGB85_15175"
     CDS             complement(4240..5199)
                     /locus_tag="VGB85_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135424.1"
                     /translation="MAYRLLLPLSCVFLITACPAKEDEVPDDSSSGSTSEAASASDTG
                     ATGPATSEIEATMGPETGPDTTTSTSGPVQTSGPEVTSDGTEDSGATETEGDTEQVPE
                     ECQLPSKTYDAFVTGPESVSVDEPCIVAASQVVADTLELAFLCPTAGVVQLTVTGGPL
                     PAVQVGETLDVFYQPEFIEPIEFPAMGLLFLRSGDQLRYGAATGFLWLTGLGMDADRL
                     AIAAGLAPLTLGVEMGPCPLVKNFEGDEPWGGDDWTCNYEALALIRLAFGDEALVQGE
                     GTSGVLAAGADSYALDVRFARRGEQCIDYSLDRIAVAVALQSP"
     gene            complement(5327..6343)
                     /locus_tag="VGB85_15180"
     CDS             complement(5327..6343)
                     /locus_tag="VGB85_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135425.1"
                     /translation="MTRLTAFTFDLRRALGRRHVLGMLGGSGALLVVGCGDGGDASDA
                     STGAASTGDASSSGDTSTGGTSTGATSTGTPTTGGSDTGSDTGSSTGDPTTGTTGPVE
                     CGGPAAAWATGGTASMTAQGCYPDPFSGGVLSCALICETTAGPCTTATTIDRRDVSEG
                     FLGLPVRLALLIVDADTCAPIPGAQVEIWHTQRTGVYSGITPSPKQCYVDDPAAATFM
                     YFRGIQTAGADGRIDFDTCFPGWYGGRAIHIHFRVIRDGQTYATSQLFFADELNTEVF
                     ATHPEYSEFGLPDITNQSDGIIGGAADMANYLLDTARMPDGAMLASKVIAVRSSLQDP
                     SCTV"
     gene            6459..8213
                     /locus_tag="VGB85_15185"
     CDS             6459..8213
                     /locus_tag="VGB85_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135426.1"
                     /translation="MRPLRTLLVLAALTGCARPAPPPVAPAPSPAPAPVAVAPAPALQ
                     IRLLPRLTPTPRLGVEIVAQGLVDRAWKVAALVAADLRDLQINDDDGPLAFASRRDGL
                     AMHVTLGRAPRGPLRISYVLHGEPVEVATQDVPAALTLRIDNDRVLASGEELLLLPAA
                     ARPDPIGLRLELAPEGPIVRLVSTLGVEPGTPARARLEDLRHALFLAGTTGHALLRSP
                     AGEDDFAWTGDPMFDLRWSAAETAGTRTAVDAYFGATPEEVVRFTALFSVELDLPGVT
                     GAQVLPRGGGLYVALPPGAGWDAQARLAVTQGLVHRWLGGRLRLQAGAGDPPEAGAWF
                     ADGFARFVAREVLFDLGTLSLEDYADEVNGHHAELVTSRSRAATIAALASAAATGDHD
                     AAALLLARGVLYATRIDALIRAKHRGARSLRALLRELVAEARARNVGALPLQAFTDRL
                     RGELGEAEVALFQRTVLGGEPLTLPADALGPCFTRGPRTYTRFELGFDEAASRAVTPG
                     QVRGVRPGGPAARAGVLAGERMLALDYLPGDPGSPVELTLERDGREQVVSYRPVGGTA
                     RGDAWRRRPGIDEARCVR"
     gene            8354..9628
                     /locus_tag="VGB85_15190"
     CDS             8354..9628
                     /locus_tag="VGB85_15190"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0135427.1"
                     /translation="MFLSACNGGGSTTTESSGSDTDTGGAETNGAGSTTSPGTAAESS
                     TTGATEDPTTAGTVEPTTPTTGGMTTGDTGFCGDGILDDGEQCDDGNQVDDDACPNSC
                     NGGSCGDGIVSPGEACDDGNEDNSDACLNNCVPAACGDGIVGPGEACDDGNDIDDDDC
                     SNICALAGCGDGVIQAGEECDDANSSDTDDCLTTCKLAICGDALVLEGTEQCDDGNQT
                     DDDACLGDCTLATCGDGIVQQGVEACDDGNTNNADACSNACVVATCSDAIQNSSETDV
                     DCGGGTCPDCVDGKLCEVASDCASGTCTNGKCAQLTLMVPNCMAANVTAAVAYPMVVQ
                     PACNCHQGGQGGLSMTSAATLVSNNVNVDATQADMKRVTPGNVDQSYLLYKVFNQHNN
                     VPNGGGTKMPPGPNLSAAQQCLLVNWVKSGAK"
     gene            complement(9632..11644)
                     /locus_tag="VGB85_15195"
     CDS             complement(9632..11644)
                     /locus_tag="VGB85_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135428.1"
                     /translation="MKAAEVAPVDREAIVAELLAKHGEGQRARIERGVAQVAALWRAE
                     DGDLAAFVREQFIADPAQLDATFARLEAVFEQIDGHTHEVGREVRRPSDVDVGPLLAV
                     DPLLAAYDPFAHVTEDLFKTGPGFVVLLNWPLTTLAERDAEGPKYTRRQWAEARLTNR
                     FARRVPGEVRQAVTRASAEADRYVSQYNLWMHHLVDGTGARPFPSGLRLISHWNLRDE
                     LKGRYGDPEGLKKQRMIIKVMERIVTQTIPAAVIDDPRVDWDPFSNKVSAAPADTIEA
                     DAPRRDAAPVLDGAPEPDARYEKLLAQYHAARREDPHAPTAPTAIARSFELGRELPEA
                     RVKALLEQVLASPLVPRVARVIEGRLGRPLEPVDLWYAGFKAGGAPPEQALDAITRKR
                     YPDAQAFARALPEVLRGLGFAEDRARWLAERIRVEPSRGAGHALQALRRGDFPRLRTR
                     VGAGGMDYKGYNIAVHELGHNVEQVFSLDGVDHTLLAGVPGNAFTEALAFVFQARDLE
                     LLGLKKPDAASERERVLNDFWQTWEIAGVALVDVATWHWMYDHPDATPKQLREAVVRI
                     SEEIWDRHYAPVLGGSGTPLLGVYSHMISYPLYLADYPLGHLIAFQIEEHLSKSGQLG
                     PEFERMATFGAVTPDVWMTHATGAPVSAEPLLRATEAALAGGQASG"
     gene            complement(11812..13158)
                     /locus_tag="VGB85_15200"
     CDS             complement(11812..13158)
                     /locus_tag="VGB85_15200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0135429.1"
                     /translation="MPPEARWLAGGGGVAGLGLVLEEPGGGAVAYGLALVLVLASAGA
                     TPPVGPAWIWPVFLVLATGLGGLGAALWPGDPAVIASVLLLQAGAGLAAASRPVAVAG
                     AAAIAGAVVVASWPSGQVGAWLVAPVAGLGLAGTLAALLDAMAQQGLHVAEPAPAHLG
                     MTRVELEQAQRELVDVTSQLHREAHQRRQAEAQALAALKARTAFLSVMSHELRTPLNQ
                     IIGYSELLLEEMADGSPDDAAGDVERIHYASLNLLEMVTNVLDLSKIEAGTMAVAVET
                     FEVAELVENLVQSFATQAHQGGNVIRVRCPEGLAPARTDRTKLRTVLANLISNACKFT
                     KKGTIRVAVSETPEGGTHSLVVEVSDTGIGIPPEEIDRLFAAFVQADGSTTRRHDGSG
                     LGLTLAHHFCAMLGGEITVESTPDVGSTFRVRVPREFVDPRRAGHVLTSMASPAAR"
     gene            complement(13438..13596)
                     /locus_tag="VGB85_15205"
     CDS             complement(13438..13596)
                     /locus_tag="VGB85_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135430.1"
                     /translation="MHWRRETARPYRDDDRQSSTVPQACRQVTQFVPLKPAKRLKDGK
                     PLGDVKAG"
     gene            13836..>14276
                     /locus_tag="VGB85_15210"
     CDS             13836..>14276
                     /locus_tag="VGB85_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135431.1"
                     /translation="MTGDPHHIRARADGKAPAGPRCDDLDLHRIDLAARLLALKQPSL
                     SSESLIVRTASEAGSSGGELDAALFIAEMIRVSGQMPGIDDGARSLLAAERLAELLDA
                     LSDPADVVALRGLRRRGELDRPLPLTSQRRIRRACWLMHAAGNGA"
BASE COUNT         2100 a         5316 c         4826 g         2034 t
ORIGIN      
        1 gcgagtcgcc ggtcggtccg atggtcgacg tcagcgaccc gcagctgtac gagttctcgt
       61 tcaaggccag cgacgccgac ccggaggcgt cgctggcact cggcgcgcag ctggccgagc
      121 tcgacacgac ggtcgcgccg atcggcaagc tggtggtgta tctacacggc gcgggcgagc
      181 cggagacctg cgggtccaag ccgcacgggg tcgtgctggc gaagatgggc tttcacgtgc
      241 tgcagccgtg ctacgtcagc ggttacgggg tcgacaagtg caaggacgac atcggtggct
      301 gtcggctcga ggccttcgag ggcgtcgacc atcaccctgt catcgacatc acgccgccga
      361 acagcatcga gacgcgggtg gtcaggggcc ttgagtactt gcaggcgatg cacccgggcg
      421 gtgactggca gtacttcatc gtcgacggcg agccgcgctg gtcgcagatc atcatctccg
      481 gcatctcgca cggcgcgagc tcctcgggcg tgatcggcat ggtccgcgag gtcgaccgcg
      541 cggtgatgct ctcgggcccg ctcgacaccg atcaggcgtg gctcaagggc gtcccgctga
      601 ccccgatcga ccgcttctac ggcttcaccc acaccgccga cccgcagcac ccgggccacc
      661 tcaagagctt cggcgacatc atgctgcccg gcgagccggt cgtcgtcgac gccgcgacgc
      721 cgccctacga cgactcgcat cgcctcgtca cctccgcgcc gaccggcgac ggccacggct
      781 cgacgcaggc cggcggcgcc tcgcccaagg acggtcagga cgcctacctg ttcctgccgg
      841 tgtggcgcac gatgtacggc gcaccatgac gtgtgacgct cacgcgccga gctgcgtcgc
      901 cgcgctcgcg gcccgcagcc gggcgaacgc cgggtgctcg ccgatcagcc gcagcgcctc
      961 ggccagcagg cggatgccgg gctcgacgcg gtgccaggtc gacacgccgc tcgggtgtgg
     1021 caggcagatg acgtcgcagt cgactccgtg gtaacgcacg cgtcgctgca cgccgacgac
     1081 ctcgacgagc ggccccttgt gcccgagcac ctgctcgatc gccaggccgc cgaccggcag
     1141 caccagccgc ggttgaagga tctgcgtctc gcgggccagg aagggctggc agcgggcgat
     1201 ctcctcgcgg tcggggcgac gatcaccgcc gccggtcgcc ttgccgggga agcagcgggc
     1261 caccgcgctc atgtacacgc cggcgcggaa catcgcctcg tcgacgccgg tcgccgcctc
     1321 gaaccagcga aacagggtgc gtccggccgt ccacgcgaag ggccggccga tcgccccctc
     1381 gtgcggcccc ggtgcttgac cgacgagcag cacctcggcc gcgatcgcgg ggccgtgcac
     1441 cgaggggcca tgcatcgacg ggcacgcctg acaggcccgg agctggcggt ggagcacggc
     1501 gagtcggcgg cgggggtccg gcatgcgggc gggcgagtta tgacccgcct gccgcgcatg
     1561 cgccagccag ccgactcagc cgggctcggg ttccttgtct cgcaccagcc acttgccggc
     1621 gagcacatcg gccatgcgat gttgctcgct ggcgacggcc tgcttttcga tcttgctgcg
     1681 cccgtggcgg atgcgctgct cctctgccag tcgcgccttg tcggcgcgcg ccttggcctt
     1741 gcgaaagcga ctcagattga tgacatcagc catgatcgtt aagaccgcgg cgagcatatc
     1801 ggacgcgtgt tgcaggtacc agcgcccttt ttgcgtcgct cgatcgctca cgaccggcgc
     1861 gtcactcgcc tactgaacgc tcgatgagca tcgctgatgg accaaaacca tcgtcaccat
     1921 cgcgacatcg accctcgcgc gacgcgacgc tctcgttcgc gagcatcgag gatcgccttg
     1981 tagcaggcct tcggcgctcg atgacggtgt ttcgcgggcg cgcctgcaaa gccgacgccg
     2041 ccgaagccgc cgcgtctggg ctatgctcgg cccctccgag gcctgactta tgcatcgata
     2101 caacttccac gcactgacct tcacctgcat cttgctcggc gcctgcggcg gcggcaagaa
     2161 tgacaccgac accgaggcga gtgacagcac tgccggcgcg accggcaccg acacgaccgc
     2221 cgacgcgacc gacgcgaccg acgcgaccga gtccgtcggc atgagctcgg cgccgaccac
     2281 ctccggcgac gacggctcgg gcagcgcgaa cccgacgcaa gacagcggga ccgcgaccga
     2341 cagcaccgcc ggcggcctga cgaccaccgg cgacacggcg accaccgacg gcgacccgga
     2401 cctgatgcag ctgtgcgagt cgtggtgcgc caggaatgcc gagtgccagc agtccgaccc
     2461 tgacggctgc gcggcggact gcttcgccga cctcggctcg caggagggca ttgtgcatcg
     2521 atgcgacgat cacgatgctc gactgcatgc tcggcatgac ctgcgagcag tgggtcgcgt
     2581 tcaccgacaa cgatgagccc ggcccgtgcg ccgccgaggc cgacgcgtcc gagaacgcct
     2641 gcgccgacga ggtctgcacc gcgagcatcg gcaccagcga ggacaataca gagtgctcgt
     2701 acagcgtcga gtgcccgaac cagccgtcac gcgagatcca gtgcgccggc gagacctgca
     2761 cctgcctcga ggacggcgag gaggtcggcc agtgcccggc gatggacgtg tgcatgaacg
     2821 tcgacgacct cacctccaag gcgatggact gctgcggctt cgtctaggcg actcactcgc
     2881 cccagatctg cgcctcggac agcccgagct cggccagctc cgcggcgcgc agctcggcct
     2941 cctcggcgca gaagaattcg tccagcggcg gcggcggcac ggcggtgccg gcgagcatcg
     3001 cgtgggcctg gccgcggtga tggatctggt gctggaacag gtgcgccagc gtgcgctgga
     3061 ccgtctcgtg cttgacgccc gtgcgccgca ggatcgccac cggcagcgcg agccggtcct
     3121 cgttcgacac gcggcacgcg gtgatcagtc gccggtcgac cgcgtgctgg gcggccagca
     3181 acgccgcgca gtcggtgaac ggctccgcgg gctcgaagaa gccgcgcgcc tccgggttga
     3241 ccggctcgcc acgtagcgcc cgctcgagcg cgtcgacgta gtaccagtcg accgtgacgt
     3301 tgtgattgag cgtcgcccgg atgctcggga aaaagctcgt ccgcgtcgcg gcgaattccg
     3361 cctgcgtcag ccgcagacac gccgagagca gacggtggtt ggcccacgcg ttgttgcagg
     3421 ccatcgtgaa aaagtggtgc gagaggctcg acattttcgc ggcgaaccat aggtgcgaag
     3481 cgtgtacgcg cgcaaggcgt ggtgtaccct gcgccgatga cttacctgcg agccctcctc
     3541 ccgaccctgc tggcgctccc gctcgcctgc ggcgacaaat cgagcgacga taccggcgac
     3601 gcttccacgt cgtccacgtc atccacatca tccacatcat ccacatcatc cacatcggcg
     3661 tcggcctcgt ccaccaccag cgccacggat gtgaccaccg gtggcacgcc gaccacggac
     3721 atcggcacga gcacgacgag cacgacctcc gacgcatcgg agtccggctc gtcggagggc
     3781 ggctccggca ccggcgcgcc ggtgtgctcg ctcgagcagg tcgcctgtga cctggccgag
     3841 agcaacggcg aattcgagga ctgcgggtcc gtcgacccgt gggacgacga cacggccgcg
     3901 tggcaggcgg cgcacgactg cgcggtcgcg gcggcgagcg agcagcgagc cttcaaggtg
     3961 gtcacctggt tgcagggcat cgacagccag atcggtgtcg cctacgcgag cctcgtcggc
     4021 cgctcctacg ggctctctca cttcggcttc gacagcgacc cctgcggcgg gggcggctgc
     4081 ggcccggtcg tgtacggcgg gtcgtgtgac ctcctcgtcg ccaccccgga ctgcatggtc
     4141 gagccgggcc aggcgtgcct gacctgcgtc aaccagggcg actcgacgca gttctgcggg
     4201 ccggagtgaa gcggcgcgcg tgcacgtgca cgcgcgaggt cacgggctct gcagcgcgac
     4261 cgccacggcg atgcggtcga gcgagtagtc gatgcactgc tcgccgcgcc gcgcgaaccg
     4321 gacgtcgagc gcgtagctgt ccgcgccggc cgcgagcacc cccgaggtgc cctcgccctg
     4381 gacgagcgcc tcgtcgccga acgcgaggcg gatcagcgcc agcgcctcgt agttgcacgt
     4441 ccagtcatcg ccgccccacg gctcgtcgcc ctcgaagttc ttcaccagcg ggcacggtcc
     4501 catctcgacg ccgagggtca gcggcgcgag gccggcggcg atcgcaaggc ggtcggcgtc
     4561 catgccgaga ccggtcagcc acagaaagcc cgtcgccgcc ccgtaacgca gctggtcgcc
     4621 gctgcgcagg aacagcaggc ccatcgccgg gaactcgatc ggctcgatga actccggctg
     4681 gtaaaagacg tcgagggtct caccgacctg cacggcgggc agcggaccgc ccgtcaccgt
     4741 cagctgcacc acacctgccg tcgggcacag gaacgcgagc tcgagcgtgt cggccaccac
     4801 ctggctcgcc gccacgatgc agggctcgtc gaccgacacg ctctcggggc ccgtgacgaa
     4861 cgcatcgtag gtcttcgacg gcagctggca ctcctcgggc acctgctcgg tgtccccctc
     4921 ggtctcggtc gcgccgctgt cctcggtgcc gtcgctggtg acctccggcc cgctcgtctg
     4981 gaccggcccg ctcgtgctcg tggtcgtatc cgggcccgtc tccggcccca tggtcgcttc
     5041 gatctcggag gtcgcgggcc cggtggcgcc ggtgtcgctg gcggaggccg cctcgctcgt
     5101 cgagccgctc gacgagtcgt cagggacctc atcctccttg gccgggcacg cggtgatcag
     5161 gaagacgcag gagagaggga gcagaagtcg gtaagccatg ccgacgtctc agcaagaccc
     5221 atgccattgc agaccgtcga acatcccctg ccattgtgac ggagcgcgtg gtccgacagc
     5281 gacaggtcta cccacgagtg acatcggtgt cgcggaggcg ctcgccctac accgtgcagc
     5341 tcgggtcctg caggctgctg cgcaccgcga tcaccttcga ggcgagcatc gcaccgtcgg
     5401 gcatgcgggc cgtatcgagc agatagttcg ccatgtcggc ggcgccgccg atgatgccgt
     5461 cgctctggtt ggtgatgtcc ggcagcccga actcgctgta ctccgggtgc gtcgcgaaca
     5521 cctccgtgtt gagctcgtcg gcgaagaaca gctgcgaggt cgcgtacgtc tgcccgtcgc
     5581 ggatcaccct gaagtggatg tggatcgcac gaccgccgta ccacccgggg aagcaggtgt
     5641 cgaagtcgat gcgaccatcc gcgcccgccg tctggatccc acggaagtac atgaacgtcg
     5701 cggccgccgg gtcatcgaca taacactgct tcggcgacgg cgtgatgccc gagtagaccc
     5761 ccgtgcgttg cgtgtgccag atctctacct gtgccccggg gatcggcgcg caggtgtcgg
     5821 cgtcgacgat caacagcgcg agccgcactg gcaggcccaa aaagccctcg ctgacgtccc
     5881 ggcgatcgat cgtggtcgcc gtcgtgcacg ggcccgcggt cgtctcgcag atcaacgcgc
     5941 agctgagcac gccccccgag aacgggtccg gataacagcc ttgcgccgtc atcgacgcgg
     6001 tgccacccgt cgcccacgcg gccgccggac cgccgcactc gacgggcccg gtcgtgccgg
     6061 tcgtcggatc acccgtgctg ctgccggtgt cactgccggt gtcactgccc ccggtggtcg
     6121 gggtcccggt ggatgtcgcg cccgtgctcg tgccgcccgt gctcgtgtca cccgagctgc
     6181 tcgcgtcgcc cgtgctcgcc gcgccggtgc tcgcatcact cgcgtcaccg ccgtcaccac
     6241 aaccgacgac gagcagcgcc ccgctgccgc cgagcatacc gagcacatgc cgccgcccga
     6301 gggcgcggcg aagatcgaag gtgaacgcgg tgagcctggt catgagtgcc tgaaagagcg
     6361 cgaagcggcg gtcagggtgg caaataaatg ccccggtgtg gcaagaaacg atcgccacca
     6421 tgttcgctct cacccccgcg cgccctgacg acgcgtgcat gcgcccgctc cgtaccctgc
     6481 tcgtgctcgc ggcgctcacc ggctgtgccc ggccagcgcc gcccccggtc gcgcccgcgc
     6541 catcgcccgc gcccgcgccc gtcgccgtcg cgccggcgcc cgcgttgcag atccgtctgc
     6601 tgccccggct cacaccgacg ccgcgcctcg gcgtcgagat cgtcgcgcag gggctcgtcg
     6661 atcgcgcgtg gaaggtcgcc gcgctggtcg ccgccgacct gcgcgacctg cagatcaacg
     6721 acgacgacgg cccgctggcg ttcgcctcgc gccgtgatgg cctcgccatg cacgtcaccc
     6781 tggggcgggc cccacgaggc ccgctgcgca tctcctatgt attgcacggc gagccggtcg
     6841 aggtcgccac gcaggacgtg cccgccgcgt tgacgctgcg catcgacaac gatcgcgtgc
     6901 tcgccagcgg cgaggagctg ctgctgttgc ccgccgccgc gcgcccggac ccgatcggcc
     6961 tgcgcctcga gctcgcgccc gaggggccga tcgttcgcct cgtgagcacc ctcggggtcg
     7021 aacccggcac cccggcacgc gcgcgcctcg aggacctgcg ccacgccctg ttcctcgccg
     7081 gcacgaccgg ccacgcgctg ctgcgcagcc ccgcgggcga ggacgacttc gcgtggaccg
     7141 gcgacccgat gttcgacctg cgctggtccg cggccgagac cgccggcacg cgcaccgccg
     7201 tcgacgccta cttcggcgcg accccggagg aggtcgtccg cttcaccgcg ctgttctccg
     7261 tcgagctcga cctgcccgga gtgacaggcg cgcaggtgct gccgcgcggc ggcggcctct
     7321 acgtcgccct gccccccggc gccggctggg acgcgcaggc ccgcctggcg gtcacgcagg
     7381 gcctggtgca ccgctggctc ggcggtcgcc tgcgcctgca agccggggcc ggagatccgc
     7441 ccgaggccgg tgcgtggttc gccgacggct tcgcccgctt cgtcgcgcgc gaggtgctgt
     7501 tcgacctcgg cacgctctcg ctcgaggact acgccgacga ggtcaacggc caccacgccg
     7561 agctcgtgac ctcacgatcg cgcgccgcga cgatcgccgc gctcgccagc gcggccgcca
     7621 cgggagacca cgacgccgcc gcgctgctgc tcgcccgcgg tgtgctgtac gcgacccgca
     7681 tcgacgccct gatccgcgcg aaacaccgcg gcgctcgctc gctgcgggcc ctgctgcgcg
     7741 agctggtcgc cgaggcccgc gcgcgcaacg tcggcgcgct gccgttgcaa gccttcaccg
     7801 accgtctgcg cggagagctc ggcgaggccg aggtcgcgct gttccagcgc accgtcctcg
     7861 gcggcgagcc gctgacactg cccgccgacg cgctcggccc gtgcttcacc cgcggtcctc
     7921 gtacatacac gcgcttcgag ctcggcttcg acgaggccgc ctcgcgggcc gtgacgcccg
     7981 gccaggtccg cggcgtgcgg cccggaggac cggccgcccg cgcgggtgtg ctcgcgggcg
     8041 aacgcatgct cgcgctcgac tacctgcccg gcgacccggg gagcccggtc gagctgacgc
     8101 tcgagcgcga cggtcgcgag caggtcgtca gctatcggcc cgtcggtggc accgcgcgcg
     8161 gcgacgcctg gcgccgccgc cccggcatcg acgaggcccg ctgcgtgcgc tgactcggtc
     8221 cacgacggcc gaccgtcgcg ctggcgcctg cgcacttcct gcttggcgcg gtctcccgtg
     8281 gcaggcatcc tgcacacctg gagtcttgat taacatgcat cgctcgtcgc gttccttgcc
     8341 cctcgtcctc ggtttgttcc tgtctgcttg caacggtggt ggcagtacca ccaccgaatc
     8401 ctccggatcc gacacggaca ccggcggcgc cgagaccaac ggcgccgggt cgaccaccag
     8461 cccgggcacc gccgccgaga gctcgaccac cggcgccacg gaagatccga ccaccgccgg
     8521 caccgtcgag ccgaccaccc cgaccaccgg cgggatgacg acgggtgaca ccgggttctg
     8581 cggcgacggc atcctcgacg acggcgagca gtgcgacgac ggcaaccagg tcgacgacga
     8641 cgcgtgcccg aattcgtgca atggcggctc ctgcggcgat gggatcgtca gcccgggcga
     8701 ggcctgcgac gacggcaacg aagacaactc cgacgcctgc ctcaacaact gtgtgcccgc
     8761 cgcgtgcggc gacggcatcg tcggcccggg cgaggcctgc gacgacggca acgacatcga
     8821 cgacgacgac tgctcgaaca tctgcgcgct cgcgggctgc ggcgacggcg tcatccaggc
     8881 cggcgaggag tgcgacgacg ccaactcctc cgacaccgat gactgcctca ccacctgcaa
     8941 gctggcgatc tgcggcgacg cgttggtgct cgagggcacc gagcagtgcg acgacggcaa
     9001 ccaaaccgac gacgacgcct gcctcggcga ctgcacgctc gccacctgcg gcgacggcat
     9061 cgtgcagcag ggcgtcgagg cgtgcgacga cggcaacacg aacaacgccg acgcgtgcag
     9121 caacgcctgc gtcgtcgcga cctgttcgga cgccatccag aactccagcg agaccgacgt
     9181 cgactgcggc ggcgggacct gccccgactg cgtcgacggc aagctgtgcg aggtcgccag
     9241 cgactgcgcc tcgggcacct gcaccaacgg caagtgcgcg cagctgaccc tgatggtccc
     9301 caactgcatg gcggccaacg tcaccgccgc ggtggcgtac ccgatggtcg tgcagccggc
     9361 ctgcaactgc catcagggcg gtcagggcgg cctgtcgatg accagcgcgg cgacgctcgt
     9421 cagcaacaac gtcaacgtcg acgccacgca ggccgacatg aagcgtgtca cgccgggcaa
     9481 cgtcgaccag agctacctgc tctacaaggt cttcaaccag cacaacaacg tgcccaacgg
     9541 cggcggcacc aagatgccgc ccggcccgaa cctcagcgcc gcccagcagt gcttactggt
     9601 caattgggtc aagagcggcg cgaagtgacg ctcacccgct cgcctggccg cctgcgagcg
     9661 ccgcctcggt cgcgcgcagc agcggctcgg cgctgaccgg cgccccggtg gcgtgggtca
     9721 tccacacgtc cggggtgacc gcgccaaagg tcgccatgcg ctcgaactcc ggcccgagct
     9781 ggccggactt actcaggtgc tcttcgatct ggaacgcgat caggtgcccg agcgggtagt
     9841 ccgcgaggta cagcggatac gagatcatgt ggctgtacac gccgagcagc ggcgtgcccg
     9901 acccgccgag caccggcgcg tagtgacgat cccagatctc ctcggagatg cgcaccactg
     9961 cctcgcgcag ctgcttcggc gtcgcgtccg gatgatcgta catccagtgc caggtcgcga
    10021 catcgaccag cgcgacgccg gcgatctccc aggtctgcca gaagtcgttg agcacccgct
    10081 cccgctcgct cgccgcgtcc ggcttcttga ggccgagcag ctcgaggtcc cgcgcctgga
    10141 acacgaaggc cagcgcctcc gtgaacgcgt tgcccggcac gcccgcgagc agcgtgtggt
    10201 cgacgccgtc gagcgagaac acctgctcga cgttgtgccc gagctcgtgc accgcgatgt
    10261 tgtaaccctt gtagtccatg cccccggcgc cgacgcgcgt gcgcagccgc gggaagtcgc
    10321 cgcggcgcag cgcctgcaac gcgtgcccgg ccccgcgcga cggctcgacg cggatgcgct
    10381 ccgccagcca gcgagccctg tcctccgcga agccgaggcc acgcaggacc tccgggagcg
    10441 cgcgcgcgaa cgcctgcgcg tcggggtaac gcttgcgcgt gatcgcgtcg agcgcctgct
    10501 ctggcggcgc gccgccggcc ttgaagcccg cgtaccacag gtcgaccggc tcgagcggtc
    10561 gcccgagccg cccttcgatc accctggcca ctctcggcac cagcggcgaa gcgagcacct
    10621 gctcgagcag cgccttgacc cgcgcctccg gcagctcgcg cccgagctcg aagctgcgtg
    10681 cgatcgccgt cggggcggtc ggcgcgtgcg ggtcctcgcg gcgcgccgcg tggtactggg
    10741 ccagcagctt ctcgtagcgc gcgtcgggct cgggcgcgcc atccaatacc ggcgccgcgt
    10801 cacgcctcgg agcatcggcc tcgatcgtgt ccgccggcgc cgcgctgacc ttgttgctga
    10861 aggggtccca gtcgacgcgc gggtcgtcga tcaccgccgc ggggatcgtc tgcgtgacga
    10921 tgcgctccat caccttgatg atcatccgct gtttttttaa accctcgggg tcgccgtagc
    10981 ggcccttgag ctcgtcgcgc aggttccagt ggctgatcag gcgcagcccg gacgggaacg
    11041 ggcgtgcacc tgtcccatcg accaggtggt gcatccacag gttgtactgc gagacgtagc
    11101 ggtcggcctc cgcgctcgcg cgcgtcaccg cctggcgcac ctcccccggc acgcgccgcg
    11161 cgaagcgatt ggtcaggcgc gcctcggccc actgccgccg cgtgtacttc ggcccctcgg
    11221 cgtcgcgctc ggccagcgtc gtcagcggcc agttgagcag cacgacgaag cccggcccgg
    11281 tcttgaacag gtcctcggtc acgtgcgcga acgggtcgta ggcggccagc agcgggtcga
    11341 ccgccagcag cggcccgacg tcgacgtcgc tgggccgtcg cacctcgcgg ccgacctcgt
    11401 gcgtgtgtcc gtcgatctgc tcgaacaccg cctcgaggcg ggcgaaggtc gcgtcgagct
    11461 gcgccgggtc ggcgatgaat tgttcgcgca cgaacgccgc gaggtcgcca tcctcggccc
    11521 gccacagcgc ggccacctgc gcgacgcccc gctcgatccg cgcgcgctgc ccctcgccgt
    11581 gcttcgctag cagctccgcc acgatcgcct cgcggtcgac cggcgcgacc tcagcggcct
    11641 tcacgggcga ctctcccgtt cgacccgaag ctgtagaaac cgaagtagtc gccggcggtt
    11701 cggcgcgggc cggctccggg cgatcgcagg ccggcagcaa cgacagcagg agcgtggcac
    11761 gaagcgcgag gcggcacacg ccggcgacca tatccgtgcg tgcgcatcgg gtcaacgcgc
    11821 ggcaggggag gccatcgagg tcagcacgtg gccggcgcgg cgggggtcga cgaactcgcg
    11881 gggcacgcgg acgcggaagg tcgagccgac gtcgggcgtg ctctccaccg tgatctcgcc
    11941 gccgagcatc gcacaaaagt ggtgcgcgag ggtcaggccg aggcccgagc cgtcgtggcg
    12001 tcgcgtcgtc gagccgtcgg cctgcacgaa ggccgcgaac agccgatcga tctcctccgg
    12061 cgggatgccg atgccggtgt cggacacctc gaccaccagc gagtgcgtgc cgccctccgg
    12121 cgtctccgac accgcgacgc ggatcgtgcc cttcttggtg aacttgcagg cgttgctgat
    12181 caggttggcg agcaccgtgc gcagcttggt ccggtcggtg cgcgccgggg ccagcccctc
    12241 cgggcagcgc acgcggatca cgttgccgcc ctgatgcgcc tgcgtggcga agctctggac
    12301 caggttctcg accagctccg cgacctcgaa ggtctcgacc gcgaccgcca tcgtgccggc
    12361 ctcgatcttc gacaggtcga gcacgttggt caccatctcg agcaggttga gcgatgcgta
    12421 gtggatccgc tcgacgtcgc cggccgcgtc gtccggcgat ccgtcggcca tctcctcgag
    12481 caacagctcg ctgtaaccaa tgatctggtt gagcggcgtg cgcagctcgt ggctcatcac
    12541 cgacaggaac gccgtgcgcg ccttcaaggc cgccagcgcc tgcgcctccg cctggcgccg
    12601 ctggtgcgcc tcgcggtgca gctggctcgt cacgtccacc agctcgcgct gcgcctgctc
    12661 gagctcgacc cgcgtcatcc cgaggtgcgc cggcgccggc tcggcgacat gcagcccttg
    12721 ctgggccatc gcgtcgagca gcgccgccag ggtccccgcg agcccgagcc cggcgaccgg
    12781 cgcgaccagc cacgccccga cctgtcccga aggccaggaa gcgacgacca ccgcgccggc
    12841 gatcgccgcg gccccggcga ccgccacggg gcgcgacgcg gccgcgagcc cggcccccgc
    12901 ctgcagcagc agcacgctgg cgatcaccgc gggatctccc ggccacagcg cggccccgag
    12961 cccgccgagc ccggtcgcga ggacgaggaa gacgggccat atccacgccg gcccgaccgg
    13021 cggcgtcgcc ccggcggacg cgagcacgag cacgagcgcg agcccgtacg cgacagcgcc
    13081 acccccgggc tcctcgagca ccagaccgag ccccgccacg ccgccgccgc ccgccagcca
    13141 gcgcgcctcc gggggcaccc gggccgacca tctgcggcgc agctcatcgc cgtgcgaggc
    13201 acgatcgtcc ggaacccgct ccgctccgct ggctcccacg cccacagagc gtcgcggagc
    13261 gagtgacgct tgtaaagcct tccccagccc ccgaaaagtc cggcccgccc ggtcacggac
    13321 acggcgcgta aaccagcgtt accatgtaag cgcggcgtcc accgatgtgc cgaatctacg
    13381 cagcacaatc catatgtgtg gagtatctgc cgggcgcggc ggcagtggcg cactcgttca
    13441 tccggctttc acgtcgccga gtggtttgcc gtctttcagg cgtttggcgg gcttgagcgg
    13501 cacaaattgc gtgacttggc ggcaagcttg cggcacagtc gacgactggc ggtcgtcgtc
    13561 ccgatacgga cgggcggtct cgcgccgcca atgcacgtac ccgggtatcc actccggtat
    13621 ccaccccggt atcacccggt atccacattt gtggccgcat acgtgctcgg tctcctggct
    13681 ccagcctacc tgctggggtc cagtcgcctc gcactatctg ctccaggtta tctccgtact
    13741 tcgctccaac aaagtgtgca gcgattgtcg ccagcagccc ccgcccatgt cgatgggaat
    13801 gaccgacgcc tcctagcata atcggatttc acaggatgac tggcgatcca catcacatcc
    13861 gtgcacgtgc cgacgggaaa gccccggccg gaccgcgttg tgacgacctg gatctccacc
    13921 ggatcgacct cgctgcgcgg ctgctggcgc tcaagcagcc ctcgctctcc tccgagtcat
    13981 tgatcgtgcg cacggcctcg gaggccggca gcagcggggg tgagctcgac gcggcgctgt
    14041 tcatcgccga gatgatccgg gtcagcgggc agatgccggg catcgatgac ggcgcccggt
    14101 cgctgctcgc cgcggagcgc ctcgccgagc tgctcgacgc cctctccgac ccggccgacg
    14161 tcgtcgccct gcgagggctg cggcgccgcg gcgagctcga ccgcccgctg ccgctgacct
    14221 ctcagcgccg gatccgtcgc gcctgctggc tgatgcacgc cgctggcaac ggcgcg
//
LOCUS       DASWNJ010000894         2330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_51868, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000894 DASWNJ010000000
VERSION     DASWNJ010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51868"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="VGB85_15215"
     CDS             complement(<1..1098)
                     /locus_tag="VGB85_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY0135432.1"
                     /translation="MTKHRTTHRALLASWTLLAAPGCLHFDTLEDTVALETHARDWRD
                     EVIYQVLIDRFADGSDGNNYLLDRENMARYHGGDWTGLEQNLDYIEELGVTTLWISPV
                     IKNIETDANVDGYHGYWAVDLTQPNAHFGNLDDLRSLVRAAHDRDMLVVIDIVTNHMG
                     QMFFYDMNLNGVPDILLQGTGTRPQGSPVPPGFDQSGFTHVTEYDPDFNPTGVQAFTS
                     LGPAGLAPIIFPYNPKTGHITPMPELFGRAETYNRKGRTFNYDDPQQLLTGDFPGGLK
                     DVNTTRCDVKLAMVDSYARWIELTDADGFRIDTVKHVEREFWRFFTQRVRQRLDRQGK
                     KKFFMFGEAFDGNDALVGQFTKNDLPETPDPK"
BASE COUNT          328 a          710 c          913 g          379 t
ORIGIN      
        1 cttcgggtcg ggggtctcgg gcaggtcgtt cttggtgaat tggccgacga gcgcgtcgtt
       61 gccgtcgaag gcctcgccga acatgaagaa cttctttttg ccctgccggt cgagccgctg
      121 gcgaacgcgc tgggtgaaga agcgccagaa ctcgcgctcg acgtgcttga cggtgtcgat
      181 gcgaaagccg tcggcgtcgg tcagctcgat ccaccgcgcg tagctgtcga ccatcgccag
      241 cttgacgtcg cagcgggtgg tgttgacgtc cttgaggccg ccgggaaaat cgccggtcag
      301 cagctgctgc gggtcgtcgt aattgaaggt gcgacccttg cggttgtagg tctccgcgcg
      361 accgaacagc tccggcatcg gcgtgatgtg gccggtcttc gggttgtacg ggaagatgat
      421 cggcgcgagg ccggcggggc cgagcgaggt gaacgcctgc acgcccgtgg ggttgaagtc
      481 ggggtcgtac tcggtgacgt gcgtgaagcc ggactgatcg aagcccggcg gcaccggcga
      541 gccctgcggg cgcgtgccgg tgccctgcag gaggatgtcg ggcacgccgt tgaggttcat
      601 gtcgtaaaag aacatctgcc ccatgtggtt ggtgacgatg tcgatcacca ccagcatgtc
      661 gcggtcgtgc gcggcgcgga cgaggctgcg caggtcgtcg aggttgccga agtgggcgtt
      721 gggctgcgtc aggtcgacgg cccagtaacc gtggtagccg tcgacgttgg cgtcggtctc
      781 gatgttcttg atcaccggcg agatccacag ggtcgtgacg ccgagctcct cgatgtagtc
      841 gaggttctgc tcgagacccg tccagtcacc accgtggtaa cgggccatgt tctcgcggtc
      901 gagcaggtaa ttgttgccgt cgctgccgtc ggcgaagcga tctatcagca cctgataaat
      961 gacctcgtcg cgccagtcgc gggcgtgggt ctcgagcgcg acggtgtcct cgagcgtgtc
     1021 gaagtgcagg cagcccggcg cggccaggag cgtccagctc gccaggaggg cgcggtgtgt
     1081 cgtgcgatgc ttggtcatga cttgctctcc tggcgcgctt ttttcgggcg cggagtctgc
     1141 ttgttcgggg ccggcagggc gcggctcggg ccggggaggg ggcggctcgg gaggcgcggg
     1201 gcgtgccagc acgaggggat cgggggcggc ttgctcgggc cgagcttgtt cgggcttggc
     1261 gagttcgggc cgagcttgtt cgggcttggc gagttcgggc ttggcgagtt cgggcgcggg
     1321 ctgggcgtgt tcgggaagcg tggggcgcgc gaaccgggcg tgttcgggag acgcggggcg
     1381 aggctgttag ggccggggag gggccgacgg gctgcgggtc cctgcgacgc gggtcctccc
     1441 aggggacatg ctcgccgccc catctggcgc gaataccaga gtcctgtcac cgaccctaca
     1501 tcgcagggac ggaagttgac ggggcgtctg cgcgtgaccc ctgggtcctc ccgctgcttc
     1561 ccccgccagt cggccccttc accccgggcg gacattgtct gcgccccgct tgtcgccggg
     1621 accgtgcgct cgcatggtcg gaggctgtcg ggcggggccg cgtgcgtcag ggcggcaggg
     1681 accgcgctga cagacgaggt gtcgagctgc tgctgcacgc ggcgactgat cgggacgaag
     1741 tccgcggcgc tggctcgaga gacacgcgac tgaagggcgg cggcgacccg acgaacgaca
     1801 tcgctgcgaa cgcggtcgtg agtgatgggc ctgcatcgcg cgacgacgtg gttgctggcg
     1861 acaagcgggg cgccgacggt gctggtccgg ggggtagggg ccgactggcg ggggaagcag
     1921 cgggaggacc cagggctcgc gcgcagacgc cccaccgccg tgcacgagcg gagagacagg
     1981 cgcggtgaaa ggaacatgtc cttccggaca ggtggggcgg cgagcacgtg ccctgggagg
     2041 acccgcgtcg cagggccccg cagcccgtcg gccccccccc ggcccagata gtctcgcccc
     2101 gcggtggccg gacacgcgga accccggtgc tcgccgagcc acgcgcccgg acacgcggga
     2161 tcccggccct cgccgagccc ggcacccgca cacgccgaac cctggccctc gccgagacct
     2221 gtggtcatcg gacgggccat agcgagcggc gaggtggtgc gaggggtgga catcgggaga
     2281 agaggggtca gtcgcggaag tagctggtcg acccgaacca ccattcggcg
//
LOCUS       DASWNJ010000895         4261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_299592, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000895 DASWNJ010000000
VERSION     DASWNJ010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299592"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="VGB85_15220"
     CDS             <1..1093
                     /locus_tag="VGB85_15220"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0135433.1"
                     /translation="SLPGVDGDVHYKLRKILQRPYSRASLLNHVPQAASLAEDVLRRE
                     RGGGPVPVVPLMRRIVTDQLGTLLLGRSVGDYLDDIGTTLRYSLNCTVTMNWPQVMLR
                     LPNYRRAKARMLELGREVLRGSDKPVDRRSDLLAELRKATEEDPNLLSPEDLRMAAMG
                     PFVAGLDTVSNSCAFLLYALLNAPEAYRRVTADADRLFADGGPTAERLQDLPALRGAV
                     QEALRLYPIAPVLVRHVRQPFEFGGYRLETGESVLIASTIVHFLEELFPDPYKFDIDR
                     HQPTPGRKPAPLAGFGLGPHTCLGSGFAEEQMALVIATLLHHARFSLVPAAGRPTITR
                     DPSPTLGPRYTVRLDGFRRAHAKGPPVAA"
     gene            complement(1097..1480)
                     /locus_tag="VGB85_15225"
     CDS             complement(1097..1480)
                     /locus_tag="VGB85_15225"
                     /inference="COORDINATES: protein motif:HMM:NF022681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HEY0135434.1"
                     /translation="MPVLAAFDYAVVRVVPRVEREEFINVGVVVHAPTRAYLGCRVEP
                     DLARLAAFAPGLDLTGLTALLAGFRRVCAGERDAGPIAALPRVERFHWLVAPRSAVIQ
                     VSPVHGGMCEDPATALDELFARRVA"
     gene            complement(1458..2237)
                     /locus_tag="VGB85_15230"
     CDS             complement(1458..2237)
                     /locus_tag="VGB85_15230"
                     /inference="COORDINATES: protein motif:HMM:NF042851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HEY0135435.1"
                     /translation="MLRRVVATRYVVPLREGGSLPAVMEADDGAAYVVKFRGAGQGAR
                     ALIAEVIAGELARALGLPVPELVLVEVDAAIGRNEGDPEIQSLVIASAGGNAGLAYLA
                     GALNFDAAARPKIAADLAAKIVAFDAFAQNMDRTAKNPNLLWWQGALWLIDHGAALYW
                     QHDWDGGLAGFDSVFQRIAGHVLLPWADPVVAQQGWLIGGLTDAAIEAAVAAVPDDWW
                     EPAAASSVAAMRDNFAARLRARRDVAPRYLEEADRARARGV"
     gene            2303..2959
                     /locus_tag="VGB85_15235"
     CDS             2303..2959
                     /locus_tag="VGB85_15235"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEY0135436.1"
                     /translation="MPRPRTPNPPTTDGATDGSLDDLLHGDGGEGLVFTGLDVQEHEL
                     RARELTGCIFRNCKLQETRWPSSRLEDCRFERCDLTRMQPRGLRAHDLQFVDCKLMGV
                     DWSELGQFPRLAFTDCVLDFASFVGLGLRKTQFVGCKIAEANFFDCDLRDASFPGCDL
                     RGSILRSCKLAKTDFSGATGLYFDPAINDSRGAIVSIETAALIAMKLGLRVAGFTAES
                     "
     gene            3159..>4261
                     /locus_tag="VGB85_15240"
     CDS             3159..>4261
                     /locus_tag="VGB85_15240"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0135437.1"
                     /translation="MLLPYPCPPDPSDSLTIAWDALREPWVTPMETCPQDPEYHAEGD
                     VWTHTKMVCEALVRLPAWQALPADERERVFAACLLHDIAKPVTTRIDDDGRIRQPGHS
                     PRGAIMVREMLWHMGVAPVLREQIAALVRAHQIPFFLIEEQEQEPARRAAQISQTVRC
                     DHLALVTRADALGRICHDPQRLLDNIDLFTEFCAEHGCLDRPWAFASDHTRFQYFRTE
                     GRDPGYAAHDDTRCEMVLMSGLPGAGKDHWIAHNLDLPVISLDALREELDVPGTGNQG
                     VVIQAAREQAREYLRRGQSFVWNGTNLSRDMRGRPIDLAAAYNARIRIVFLDTAYRRV
                     VRQNRERDATVPLAAILRMTRLWEPPDLTEAHRV"
BASE COUNT          618 a         1669 c         1384 g          590 t
ORIGIN      
        1 ctcgctgccc ggcgtcgacg gcgacgtgca ttacaagctg cgcaagatcc tgcaacgccc
       61 ctactcgcgc gccagcctgc tcaatcacgt cccgcaggcc gcgtcgctcg ccgaggacgt
      121 gctgcgccgc gagcgtggcg gtgggccggt gccggtcgtg ccgctgatgc ggcgcatcgt
      181 caccgaccag ctcggcaccc tcctgctcgg ccgcagcgtc ggcgattacc tcgacgatat
      241 cggcaccacc ctgcgatact cgctcaactg cacggtgacg atgaactggc cgcaggtgat
      301 gctgcgcctg cccaactatc gccgggccaa ggcgcgcatg ctcgagctcg gccgcgaggt
      361 cctgcgcggc agcgacaagc ccgtcgaccg gcgctccgac ctgctggccg agctgcgcaa
      421 ggcgaccgag gaggacccga acctgctcag cccggaggac ctgcgcatgg ccgcgatggg
      481 cccgttcgtc gccggcctcg acaccgtcag caacagctgc gccttcctgc tgtatgcgct
      541 gctcaacgcg cccgaggcgt atcgccgcgt cactgccgac gccgatcggc tgttcgcgga
      601 cggcggtccg acggccgagc gcctgcagga cctcccggcc ctgcgcggcg cggtgcagga
      661 ggcgctgcgc ctgtacccga tcgcgccggt gctcgtgcgt cacgtccggc aaccgttcga
      721 gttcggcgga tatcgcctcg agacgggcga gtcggtgctg atcgccagca cgatcgtgca
      781 ctttctcgag gagctgttcc cggaccccta caaattcgac atcgaccggc accagccgac
      841 gcccggtcgc aagccggcgc cgctcgcggg ctttggcctc ggcccgcaca cctgcctcgg
      901 cagcggcttc gccgaggagc agatggccct ggtgatcgcc acgctcctgc atcacgcccg
      961 cttctcgctc gtcccggccg ccggccgtcc gacgatcacg cgcgacccct cgccgacgct
     1021 cgggccgcgc tacaccgtcc gcctcgacgg ctttcgccgc gcccacgcca agggcccgcc
     1081 ggtcgccgcg tgatcgtcag gcgacgcggc gcgcgaacaa ctcgtcgagc gccgtcgccg
     1141 gatcttcaca catgcccccg tgcaccggcg acacctggat caccgcgctg cgcggcgcga
     1201 ccagccagtg aaagcgctcg acccggggca gcgccgcgat cggcccggcg tcgcgctcgc
     1261 ccgcgcagac gcggcgaaag ccggccagca gcgccgtcag tccggtgagg tcgaggcccg
     1321 gggcgaacgc ggccaggcgc gcgaggtcgg gctcgacgcg acagccgagg tacgcgcgcg
     1381 tcggcgcgtg cacgacgacg ccgacgttga tgaactcctc gcgctcgacg cgaggcacca
     1441 cccgcaccac cgcgtagtca aacgccgcga gcacgggcac ggtcggcctc ctcgaggtag
     1501 cgcggcgcga cgtcccggcg ggcgcgcagg cgggcggcga agttgtcgcg catcgcggcg
     1561 acggacgacg ccgccgccgg ctcccaccag tcgtcgggca ccgcggcgac ggccgcctcg
     1621 atcgccgcgt cggtcagccc gccgatcagc catccctgtt gcgcgacgac cgggtcggcc
     1681 cacggcaaca gcacgtgccc ggcgatccgc tggaacacgc tgtcgaagcc ggcgaggccg
     1741 ccatcccagt cgtgttgcca gtacagcgcc gcgccgtgat cgatcagcca cagcgcgccc
     1801 tgccaccaca gcaggttggg gttcttggcg gtgcggtcca tgttctgcgc gaacgcgtcg
     1861 aaggcgacga tcttcgcggc caggtcggcg gcgatcttcg ggcgggccgc ggcgtcgaag
     1921 ttgagcgcgc ctgccaggta agccaggccc gcgttgccac cggcgctggc gatcaccagc
     1981 gactggatct cggggtcgcc ctcgttgcgg ccgatcgccg cgtcgacctc gaccagcacc
     2041 agctccggga ccggcagccc gagcgcgcgc gccagctcgc ccgcgatcac ctcggcgatc
     2101 agcgcccgcg ccccctgtcc ggccccgcga aacttcacca cgtaggcggc gccgtcgtcc
     2161 gcctccatga ccgcgggcag cgatccgccc tcacgtagcg gcacgacata acgagtggcg
     2221 acgacgcggc gaagcacggc cgcattgtac ccgcgtaccg cccgcccgcg aagcacgccg
     2281 tgctagcttc gcgcgcccga ccatgccgcg cccacgcacc ccgaacccgc cgaccaccga
     2341 cggcgccacg gacggttctc tcgacgacct gctgcacggc gacggcggcg agggcctcgt
     2401 gttcaccggc ctcgatgtcc aggagcacga gctgcgcgcc cgggagctga ccggctgcat
     2461 ctttcgcaat tgcaagctgc aagagacccg ctggccgtcg agtcgcctcg aggactgtcg
     2521 cttcgagcgc tgcgacctga cgcgcatgca gccccggggg ctgcgcgcgc acgacctgca
     2581 atttgtggat tgcaagctga tgggcgtcga ttggtcggag ctcggccagt tcccgcggct
     2641 cgccttcacc gactgcgtcc tcgacttcgc ctccttcgtc ggcctcggcc tgcgcaagac
     2701 ccagttcgtc ggctgcaaga tcgccgaggc caacttcttc gactgcgacc tgcgcgacgc
     2761 cagctttccc ggctgcgacc tgcgcgggag catcctgcgc agttgtaagc tcgccaagac
     2821 cgacttctcc ggcgccaccg gcctgtactt cgaccccgcg atcaacgact cgcgcggcgc
     2881 gatcgtctcg atcgagacgg ccgcgctgat cgccatgaag ctcggcctgc gcgtcgccgg
     2941 cttcaccgcc gagtcatgac gcggccgccg cggaaccgcc caatctccaa gcccacgcgc
     3001 cctcaacgcc acgaaagcgc gcccctcacc gtcccgcccg cccgcgatcc gcatcttcac
     3061 gcgacggcgc gggccctggc ggctaaagtg tcccgcccat gttcgccggc tccgctcgcc
     3121 cgcgtatcgc cggcgacgca gcgggtccat gtgacgccat gctgctccct tatccctgcc
     3181 cacctgaccc ttcggacagc ctgacgatcg cctgggacgc cctgcgcgag ccgtgggtca
     3241 cgccgatgga gacgtgcccg caggacccgg agtatcacgc cgagggcgac gtctggaccc
     3301 acacgaagat ggtctgcgag gccctggtgc gcctgccggc ctggcaggcc ctgccggccg
     3361 acgagcgcga gcgggtgttc gccgcctgtc tgctgcacga catcgccaag ccggtcacca
     3421 cgcgcatcga cgacgacggc cggatccgcc agcccggcca ctcgccgcgc ggggcgatca
     3481 tggtgcgcga gatgttgtgg cacatgggcg tcgcgccggt cctgcgcgag cagatcgccg
     3541 ccctcgtacg cgcgcaccag atccccttct tcctgatcga ggagcaggag caggagccgg
     3601 cgcgccgcgc cgcgcagatc agtcagacgg tccgctgcga tcacctcgcg ctggtcaccc
     3661 gcgccgacgc gctcggacgg atctgccacg acccgcagcg tctgctcgac aacatcgacc
     3721 tcttcaccga gttctgcgcc gagcacggct gcctcgatcg cccgtgggcc ttcgccagcg
     3781 atcacacccg cttccagtat tttcgcaccg agggccgcga ccccggctac gccgcgcacg
     3841 acgacacccg ctgcgagatg gtgctgatga gcggcctgcc cggcgccggc aaggaccact
     3901 ggatcgcgca caacctcgac ctgccggtca tctcgctcga tgctttgcgg gaagagctcg
     3961 acgtgcccgg caccggcaat cagggcgtcg tcatccaggc cgcgcgcgag caggcgcgcg
     4021 agtatctgcg caggggccag tcgttcgtgt ggaacgggac caacctcagc cgcgacatgc
     4081 gaggccgccc gatcgacctc gccgccgctt acaacgcccg catccggatc gtcttcctcg
     4141 acactgccta ccgccgcgtc gtccgtcaga accgcgagcg cgacgcgacc gtcccgctcg
     4201 ccgcgatcct ccgcatgacc cgcctgtggg agccgccgga cctcaccgag gcccaccgcg
     4261 t
//
LOCUS       DASWNJ010000896         2718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_547367, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000896 DASWNJ010000000
VERSION     DASWNJ010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547367"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VGB85_15245"
     CDS             complement(<1..869)
                     /locus_tag="VGB85_15245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0135438.1"
                     /translation="MVGAALVLALLHIPACGGEAGANAPGKGPGGAGKDAAPQEPLAV
                     AVTAIAPTAIDRFYRTSGTLRALRSADLVALQPGVVLDLRAEEGDRVRAGQVLVRLDG
                     RGFKLQAARDALTARNAQQELERLKQLADHLSREELDKQRYALENALASAQVSKYQAS
                     QTTVVAPFDGTITRRSIDIGNLATASTPLYSLADLSVLDVDLHVPEREAAAVQAGATA
                     TLELQDGTRFAGRVERRAPVVDPLTGTVKFTVRAKEYPVGAVPGAFVRAELLVDRRAD
                     APAVPRTAIFELEG"
     gene            1042..1647
                     /locus_tag="VGB85_15250"
     CDS             1042..1647
                     /locus_tag="VGB85_15250"
                     /inference="COORDINATES: protein motif:HMM:NF025255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4203 domain-containing protein"
                     /protein_id="HEY0135439.1"
                     /translation="MVVPGNSFSAALGDLLGASPALTLVLGLVHVVAGYRCWVGLLGI
                     YGLVFTATIGAAFAERWFPGQDLAYRLMQVGAGLFGFGAVLAFHRVGVFILGALAGAV
                     VAELIATGIGTGITDPVLMLAAMIGGSLAVATERGALILATSITGAALVVRSAVALGH
                     GPAAWSLGSLDLWTWTTPTLGAELAAMGVLVLVGIFIQRRG"
     gene            complement(1695..>2718)
                     /locus_tag="VGB85_15255"
     CDS             complement(1695..>2718)
                     /locus_tag="VGB85_15255"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135440.1"
                     /translation="MIFGTPTYMSPEQAQGQRVDARSDIYALGVILYELLTAKVPFSA
                     DNFMGILTKHMFDEPMAPSEAAPYADISPEVEAIVLKALQKERAYRFQTMEELSAAIV
                     AVGTGAEPVTVIPEARARPISEGRPTAFLTRSSSGAMTRVAGEVTIAERPRRSRGLLV
                     AVAAGFAMLGGVATLLALGDEDPHAQPVGVPLEPVPVLSVSPPAPAPEPEPAPTVEPT
                     PAPDARVYVASNVPAEVIDAADGTVLGATGDAGFTLPQAAGPRKVLVRAAGHDDLALE
                     LEPVADRRFEAVLRPKKKIVASKTGKPGDGKKPESPVVDPKPAEPRPVGSMGLKNPFE
                     TRTIPP"
BASE COUNT          308 a         1032 c          959 g          419 t
ORIGIN      
        1 ccctcgagct cgaagatcgc cgtgcgcggc acggcgggcg cgtcggcgcg gcggtcgacg
       61 agcagctcgg cccgcacgaa cgcgcccggc accgcgccga ccgggtactc cttcgcacgc
      121 acggtgaact tgacggtgcc ggtcagcggg tcgacaaccg gggcgcgccg ctcgactcgg
      181 ccagcgaagc gcgtgccgtc ctgcagctcg agcgtcgccg tcgccccggc ctgcaccgcc
      241 gcggcctcgc gctccggcac gtgcaggtcg acgtcgagca cgctgaggtc cgcgaggctg
      301 tacagcggcg tcgaggcggt cgccaggttg ccgatgtcga tcgatcgccg cgtgatcgtg
      361 ccgtcgaagg gcgcgacgac ggtggtctgg ctggcctggt acttcgacac ctgggcgctc
      421 gccagcgcgt tctccagcgc gtaacgctgc ttgtcgagct cctcgcgcga gaggtggtcg
      481 gcgagctgct tgagccgctc gagctcctgc tgcgcgttgc gggcggtcag ggcgtcgcgc
      541 gcggcctgga gcttgaagcc gcgaccgtcg aggcggacca gcacctgccc ggcgcgcacg
      601 cgatccccct cctcggcccg caggtcgagc acgacccccg gctgcagcgc gaccaggtcc
      661 gccgagcgca gcgcccgcag cgtccccgag gtccggtaga agcggtcgat cgccgtcggg
      721 gcgatcgcgg tgacggcgac cgccagcggc tcctgcggcg ccgcatcctt gccggcgcca
      781 cccgggcctt taccaggcgc gttggccccg gcttccccgc cacacgccgg tatatgcaac
      841 aacgcgagca cgagggccgc cccgaccatc cggccccgaa cccgtgctgc gctgcgcccg
      901 atcatccgtg cttcgacccg atccggccgc acgttgttgc ggcccgcgat ccttagcacg
      961 gtgccgccgg tcgcggccac cggcccccgg agcgcgcccc ggtttcttgg agcccggcgc
     1021 gcgcggcgtt agcttcgcgc cgtggtcgtt ccaggcaaca gctttagcgc ggcgctcggc
     1081 gatctgctcg gcgcctcccc tgccctcacc ctcgtcctcg gactcgtcca cgtcgtcgcc
     1141 ggctatcgct gctgggtcgg cctgctcggc atctatggcc tggtgttcac cgcgacgatc
     1201 ggtgcggcct tcgccgagcg ctggttcccc ggtcaggacc tcgcctaccg cctcatgcag
     1261 gtcggcgccg gtctgttcgg cttcggcgcc gtgctggcct tccaccgcgt cggcgtgttc
     1321 atcctcggcg ccctcgccgg tgccgtcgtc gccgagctga tcgccaccgg gatcggcacc
     1381 ggcatcaccg acccggtcct gatgctcgcc gcgatgatcg gcggctcgct cgcggtcgcc
     1441 accgagcgcg gcgccctcat cctcgccacc tcgatcaccg gcgccgccct cgtcgtccgc
     1501 agcgccgtcg ccctcggcca cggccccgcc gcctggtccc tcggatccct cgacctctgg
     1561 acctggacca cccccaccct cggtgcggag ctcgccgcga tgggcgtgct cgtgctcgtc
     1621 gggatcttca tccagcgtcg tggctagtta gccagcgccg aggccagtta gctcgccccg
     1681 ctccgggcga gcgatcaagg cgggatcgtg cgcgtctcga acgggttctt gagccccatc
     1741 gatccgacgg gcctgggctc ggccggcttt ggatcgacga ccgggctctc gggcttcttg
     1801 ccgtcgcccg gcttgccggt cttgctggcg acgatcttct tcttcggccg caacaccgcc
     1861 tcgaagcggc ggtcggcgac cggctcgagc tcgagcgcga ggtcgtcgtg gcccgccgcg
     1921 cgcacgagca ccttgcgcgg accggccgcc tgcgggagcg tgaagccggc gtcaccggtc
     1981 gcgccgagca cggtgccgtc cgcggcgtcg atcacctcgg cggggacgtt gctcgccacg
     2041 tacacgcggg cgtcgggcgc cggcgtcggc tcgaccgtgg gcgcgggctc gggctcgggc
     2101 gcaggcgcgg gcgggctgac cgaaaggaca ggcaccggct cgagcggcac gccgaccggt
     2161 tgcgcgtgcg ggtcctcgtc gccgagcgcc agcagggtcg ccacgccgcc gagcatcgcg
     2221 aagccggccg ccacggcgac cagcaggccg cggctgcggc gtggtcgctc ggcgatcgtc
     2281 acttcaccgg cgacccgcgt catcgcgccc gagctcgagc gggtgaggaa cgcggtcggt
     2341 cggccctcgc tgatcggccg cgcgcgggcc tcggggatca cggtgactgg ttcggcgccg
     2401 gtgccgacgg cgacgatcgc cgccgacagc tcctccatgg tctggaaccg gtaggcgcgc
     2461 tccttttgca gcgccttgag cacgatcgcc tcgacctcgg ggctgatgtc ggcgtacggg
     2521 gcggcctcgc tgggcgccat cggctcgtcg aacatgtgct tggtcaggat ccccatgaag
     2581 ttgtccgccg agaacggcac cttggcggtc aacagctcgt agaggatcac gccgagcgcg
     2641 tagatgtcgg accgcgcgtc gacgcgctgg ccctgggcct gctccgggct catgtaggtc
     2701 ggcgtgccga agatcatg
//
LOCUS       DASWNJ010000897         2038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_157732, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000897 DASWNJ010000000
VERSION     DASWNJ010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157732"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VGB85_15260"
     CDS             <1..530
                     /locus_tag="VGB85_15260"
                     /inference="COORDINATES: protein motif:HMM:NF021354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BPL-N domain-containing protein"
                     /protein_id="HEY0135441.1"
                     /translation="GRQLREAGRARVIDFVRNGGGYIGICAGAYLAMQGGDTSQKLLL
                     VAARNRTGGAWRRGVAGVWVEAEGRPRFRLFYANGPIFTPDTTGGAPYVELARYAGEV
                     FSRARGTGPGEMLGTPAIVAAASGQGRVLLFSPNPILGGQGVAHPELMRAGLRWTAAG
                     GAVPDGLEFGDVFPG"
     gene            complement(695..>2038)
                     /locus_tag="VGB85_15265"
     CDS             complement(695..>2038)
                     /locus_tag="VGB85_15265"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0135442.1"
                     /translation="LPPGPRFAALQTWLYARDPIGHLARCQRRYGDPFTVPTTAGPVV
                     VAATPALVREVFAADADVFVPFAPHTVELFVGRGSLLVQAGAQHREARRRISPALHGE
                     ALRARGGEIRDVAVTSARAWRPGRVFVLLDAARGVSRELIVRAVLGLRDPRRIEALGR
                     ALAAMLAAGTPALLFVPTLRRRIGGLGPFARFARAQARVHALLSAEIAARRACPEGQD
                     VLTTLLGELDDETVRDELLTLLIAGHETTGVALAWAVYWLLRSPTALARVLAELDACD
                     ATPDALARLPYLDAVCHEALRLGPVVSDVTRTLARPLRLGGYDLPAGTSVAAATALLH
                     TRSDLYPRPGEFRPERFLERRFGPCEHAPFGGGARRCPGAAFALYSLKIVLGTWLARH
                     TLRLAEPGDVAPARRNLVLGPSTGVRVVGFPRASRSTDDRRSLAAAKWPTSSRLL"
BASE COUNT          288 a          732 c          789 g          229 t
ORIGIN      
        1 agggtcggca gctccgcgag gccgggcggg cccgggtcat cgatttcgtg cgtaacggcg
       61 gcggatatat cggcatctgc gccggcgcct atctcgcgat gcagggcggt gatacctcgc
      121 aaaagctcct cctggtcgcc gcccgcaacc ggaccggggg cgcgtggcgg cgcggcgtgg
      181 ccggcgtgtg ggtcgaggcc gagggccgac cccggtttcg cctgttttat gccaatggcc
      241 cgatctttac gcccgacacg accgggggtg ctccctacgt cgagctggca cgttacgcgg
      301 gcgaggtctt cagccgcgcg cgcggcactg gtccgggcga gatgctcggc acgccggcga
      361 tcgtcgcggc ggcgagcggt cagggccgcg tcttgctgtt ctcgccgaac ccgatcctcg
      421 gcggccaggg cgtcgcgcac cccgagctga tgcgcgccgg tctgcgctgg acggccgccg
      481 gcggcgcggt gcccgacggc ctcgagttcg gcgacgtgtt cccgggctga gccgggccgc
      541 tcacgcgtcg aacatgcggc gaaaaggtct gcacggtcgg cccggtcggg ggtgcagccg
      601 gcgaggctcg acgcgacgag gacggacagg aggacgcggc agagtcacga gcgggcgagt
      661 gccatggctg ggttccccgg gtgcgattca cggatcacag caggcgcgac gaggtcggcc
      721 attttgcggc ggcgagggag cggcggtcgt cggtcgatcg tgaggcgcgc gggaagccga
      781 ccacgcgcac cccggtcgac ggcccgagca caaggttgcg tcgcgccggc gcgacgtcgc
      841 cgggctcggc caggcgcagc gtgtggcgag ccaaccacgt gccgagcacg atcttcagcg
      901 agtacagggc gaacgcggcg cccgggcagc ggcgcgcgcc gccgccgaac ggcgcgtgct
      961 cgcacggtcc aaagcggcgc tcgaggaagc gctcgggacg aaactctcct ggccgcggat
     1021 agaggtccga ccgcgtgtgc agcagcgcgg tcgcggccgc gaccgacgta cccgccggca
     1081 ggtcgtaccc accgagccgc agcggacgtg ccagcgtgcg cgtgacatcc gagacgaccg
     1141 ggccgaggcg cagggcctcg tgacacacgg cgtcgaggta cggcagccgc gccagcgcgt
     1201 cgggtgtggc gtcgcaggcg tcgagctcgg ccagcacgcg cgcgagcgcg gtggggctgc
     1261 gcagcagcca gtacaccgcc cacgcgagtg cgacaccggt ggtctcgtgc ccggcgatca
     1321 gcagggtcag cagctcgtcg cgcactgtct cgtcgtcgag ctcgccgagc agcgtcgtga
     1381 gcacatcctg tccttcagga caggcgcgcc gcgccgcgat ctcggcggac agcagcgcgt
     1441 gcacccgggc ctgggcgcgg gcgaagcgag cgaaggggcc gaggccgccg atgcgccggc
     1501 gcaaggtcgg cacgaacagc agcgcgggtg tacccgccgc gagcatcgcc gcgagcgccc
     1561 ggccgagcgc ctcgatgcgc cgggggtccc gcaggccgag caccgcacgc acgatcagct
     1621 cgcgggagac gccacgcgcc gcgtcgagca gcacgaacac acggccgggc cgccacgcgc
     1681 gggcgctcgt gacggccacg tcccggatct ccccgccacg cgctcgcaga gcctcgccgt
     1741 gcagcgccgg cgagatccgc cggcgcgcct cccggtgctg cgccccggcc tggaccagca
     1801 gcgagccgcg cccgacgaac agctcgaccg tgtgcggcgc gaacgggacg aacacgtccg
     1861 cgtcggcggc gaagacctcc cggaccagcg ccggcgtcgc ggcgacgacg accgggcccg
     1921 ccgtcgtcgg caccgtgaag gggtcgccat agcgccgctg acaccgcgcg aggtggccga
     1981 tcgggtcacg cgcgtacagc caggtctgca gcgccgcgaa ccgcggtccg ggcggcag
//
LOCUS       DASWNJ010000898         1578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_328024, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000898 DASWNJ010000000
VERSION     DASWNJ010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328024"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VGB85_15270"
     CDS             complement(<1..292)
                     /locus_tag="VGB85_15270"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0135443.1"
                     /translation="MSYETFHDVQRQLEQLVGDLDAATPRGKTRARILDAAAQQFVRH
                     GYRKASIDEIAKQAGIGKGTLYLHFATKTDVLVAALAREKLRALDLLRAAFSN"
     gene            367..1047
                     /locus_tag="VGB85_15275"
     CDS             367..1047
                     /locus_tag="VGB85_15275"
                     /inference="COORDINATES: protein motif:HMM:NF016879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Coq4 family protein"
                     /protein_id="HEY0135444.1"
                     /translation="MSKWSQYREILRARREGAPLADLAVMKFAWYVDRASPLNEQIRG
                     LDEVYLDIDIDALRRLPEGTVGRAYARHLDDHELQPMKVSPEIQRRYADNPLPLRYTT
                     THDLFHVLTGFPTTPAGELGLFAFMVGQGFASMKRLRQAEVVYAIVMPLHAVGVRRNV
                     RAGLAMARRAAPLLAQPMRPLLGQPLAQVRRQLGIPTPEEAGIHPGHESLLSRWLLPK
                     PSSQAATA"
     gene            complement(1055..>1578)
                     /locus_tag="VGB85_15280"
     CDS             complement(1055..>1578)
                     /locus_tag="VGB85_15280"
                     /inference="COORDINATES: protein motif:HMM:NF023367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HEY0135445.1"
                     /translation="RWDNEWAGSGTEQWDAPPTLPAGDTAFERLANGYTVMMTLNGIP
                     LIYYGDEIGLAGAGDPDNRRMMEFDNTKYTSGQKLLLAHVQKLTKLRKAHPALSRGAR
                     TTLSVTDDTFVYEKVDATADDQIIVVLNRSDTMQDVMGVPDGQYTDLLTDTPVTGGTI
                     SVPPRSSMLLAPQ"
BASE COUNT          224 a          492 c          586 g          276 t
ORIGIN      
        1 cgttgctgaa ggcggcgcgt agcaggtcga gcgcgcgcag cttctcgcgc gcgagcgcgg
       61 cgaccagcac gtcggtcttg gtggcgaagt gcaggtacag ggtgcccttg ccgatgccgg
      121 cctgtttggc gatctcgtcg atgctggcct tgcggtagcc gtggcgcacg aactgctgcg
      181 cggcggcgtc gaggatccgc gcgcgcgtct tgccgcgcgg ggtcgcggcg tcgaggtcac
      241 ccaccagctg ctcgagctgg cgctgcacgt cgtgaaatgt ctcgtaactc acggatgacc
      301 attttgacta atttggtcac cgagtcaatt tggtttttga gggcgtgcaa caaggagtac
      361 agggccatga gcaagtggag tcagtaccgg gagatcctgc gggcgcggcg cgaaggcgcg
      421 cctctggcgg acctcgccgt catgaaattc gcgtggtacg tcgaccgggc gtcacccctc
      481 aacgagcaga tccgtgggct cgacgaggtg tacctcgaca tcgacatcga cgcgctgcgc
      541 cggctgccgg agggcacggt cggccgggcc tacgcgcggc acctcgacga tcacgagctg
      601 cagccgatga aggtcagccc ggagatccag cggcgttacg ccgacaaccc gctgccgctg
      661 cgctacacga ccacgcacga cctgtttcac gtgctgacgg gcttcccgac cacgccggcg
      721 ggggagctcg ggctgttcgc gttcatggtc gggcagggct ttgcgagcat gaagcggctg
      781 cgtcaggccg aggtcgtgta cgcgatcgtg atgccgctac acgcggtcgg cgtgcgccgc
      841 aacgtgcggg ccggcctggc gatggcgcgc cgggccgcgc ccttgctcgc ccagccgatg
      901 cggccgctgc tcgggcagcc gctcgcgcag gtgcggcgtc agctcggcat cccgacgccg
      961 gaggaggccg gcatccaccc cgggcacgag agcctgctgt cgcggtggtt gctgccgaag
     1021 ccgtcgtcgc aggcggcgac ggcctgagca cgcgctattg cggcgcgagc agcatcgagc
     1081 tgcgcggggg cacggagatc gtgccgccgg tcaccggggt gtcggtgagc aggtcggtgt
     1141 actggccgtc gggcacgccc atgacatctt gcatggtatc gctgcggttg aggacgacga
     1201 tgatctggtc gtcggcggtc gcgtcgacct tttcataaac aaaggtgtcg tcggtgaccg
     1261 acagcgtggt gcgggcgccg cggctgagcg cggggtgggc cttgcgcagc ttggtcagct
     1321 tctgcacgtg ggcgagcaac agcttttgcc cgctggtgta cttggtgtta tcaaattcca
     1381 tcatgcggcg gttgtcgggg tcgccggcgc cggcgaggcc gatctcgtcg ccgtagtaga
     1441 tcagcgggat gccgttgagg gtcatcatca cggtgtaacc attggcgagg cgctcgaagg
     1501 cggtgtcgcc ggccgggagc gtgggagggg cgtcccactg ctcggtgccg ctgccggccc
     1561 actcgttgtc ccagcgcg
//
LOCUS       DASWNJ010000899         1546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_547459, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000899 DASWNJ010000000
VERSION     DASWNJ010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547459"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VGB85_15285"
     CDS             complement(<1..756)
                     /locus_tag="VGB85_15285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEY0135446.1"
                     /translation="MSRTAPTEITEEMLRGWPLPALDGKLGKVERGKVLVFGGSDEVP
                     GAAILAAVAALRVGAGTLQIATTRRLAPLVAVTVPEARVIGLPSTRVGQIAGSAAAKL
                     HRELDRANAVLLGPGMVDPAGALAMLALHHKRKAKAALVLDAGALLALCAKKRPPVGE
                     RTIVTPHAGEMAKLAGIDRDEVLDRPHALACETAARLGVIVALKGAHTYVAAPDGTCF
                     LNTAGNLGLGTSGSGDTLSGVIAGLCARGAEPLQ"
     gene            complement(753..>1546)
                     /locus_tag="VGB85_15290"
     CDS             complement(753..>1546)
                     /locus_tag="VGB85_15290"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY0135447.1"
                     /translation="ARFAPVASSIEQVWPTRLWLVRHGESAGNVARDIAEAGGFPTID
                     IAERDVDVPLSALGERQARAVGRWFASQPESERPGVILSSPYVRAMQTAKHIIEGGDL
                     GCSALVHVADERLREKEFGCLDRLTRAGITAKYPEEAERRAHVGKFYYRPPAGESWCD
                     VILRLRSMFDHIQLQYRKERVLIVAHQVTVLCARYLLEHMSEAQILAIDREKDVANCS
                     VTTYDFAIDPEGRGGLKLRGYNYVAPIEDAGEPVTKQPDAPVSPR"
BASE COUNT          202 a          600 c          525 g          219 t
ORIGIN      
        1 ctgcagcggc tcggcccccc gcgcgcacag ccccgcgatc acccccgaga gcgtgtcgcc
       61 cgagcccgag gtcccgagcc cgaggttgcc ggccgtgttc aaaaaacagg tcccgtccgg
      121 cgccgcgacg tacgtgtgtg cgcccttgag ggccacgatc accccgagcc gcgccgccgt
      181 ctcgcaggcg agcgcgtgcg gccgatcgag cacctcgtcg cggtcgatcc cggcgagctt
      241 ggccatctcg ccggcgtgcg gcgtcacgat cgtgcgctcg ccgaccgggg ggcgcttctt
      301 ggcgcacagc gccagcaggg cccccgcgtc gagcaccagc gccgccttgg ccttgcgctt
      361 gtgatgcagc gcgagcatgg cgagcgcgcc ggccgggtcg accatgccgg gcccgagcag
      421 caccgcgttc gcgcggtcga gctcgcggtg cagcttcgcc gcggccgacc ccgcgatctg
      481 acccacgcgc gtgctcggca gcccgatcac ccgcgcctcg ggcaccgtca ccgcgaccag
      541 cggcgccagt cgccgcgtcg tcgcaatttg cagcgtgccg gccccgacgc gcagcgccgc
      601 gacggccgcg aggatcgccg ccccgggcac ctcgtcggat ccgccgaaca ccagcacctt
      661 gccgcgctcg accttgccga gcttgccgtc gagggccggc agcggccagc cgcgcagcat
      721 ctcctccgtg atctccgtcg gcgcggtgcg gctcatcgtg ggctcaccgg cgcgtcgggc
      781 tgcttcgtga ccggctcgcc ggcgtcctcg atcggcgcga catagttgta gccgcgcagc
      841 ttgagcccac cgcggccctc ggggtcgatc gcaaagtcat aggtcgtcac cgagcagttg
      901 gcgacgtctt tctcgcggtc gatcgccagg atctgcgcct cgctcatgtg ctcgagcaag
      961 tagcgcgcgc acagcacggt gacctggtgc gcgacgatca gcacccgctc cttgcggtac
     1021 tgcagctgga tgtgatcgaa catgctgcgc aggcgcaaga tgacgtcgca ccagctctcg
     1081 ccggccggcg gccggtagta gaacttgccg acgtgggcgc ggcgctcggc ctcctccggg
     1141 tacttcgcgg tgatgcccgc gcgcgtcagg cggtcgaggc agccgaactc cttctcgcgc
     1201 aggcgctcgt cggcgacgtg cacgagcgcc gagcagccga ggtcgccgcc ctcgatgatg
     1261 tgcttcgccg tctgcatcgc gcgcacgtac ggcgagctca ggatcacccc cggccgctcc
     1321 gactccggct gcgacgcgaa ccaccgcccg accgcgcggg cctgacgttc gccgagggcc
     1381 gacagcggga cgtcgacgtc gcgctccgcg atgtcgatcg tcgggaagcc gcccgcctcg
     1441 gcgatgtccc gcgcgacgtt gcccgcgctc tctccgtgac gcacgagcca cagccgcgtc
     1501 ggccacacct gttcgatcga ggacgccacg ggcgcgaacc tagcac
//
LOCUS       DASWNJ010000900         3495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_475272, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000900 DASWNJ010000000
VERSION     DASWNJ010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475272"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VGB85_15295"
     CDS             <1..433
                     /locus_tag="VGB85_15295"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135448.1"
                     /translation="DAREVEFSVLGNGDESTIVSGPGEIGLPPGTWYDYDNKYIKDVA
                     TLQIPAALPDELTARMQDICLRAFRLTGCKGLARVDFLVDRTTLEPYLNEINTMPGFT
                     SISMYPKLLGHIGLGYTEVLTRLCDLALTHHADRQKLSVTR"
     gene            446..1747
                     /locus_tag="VGB85_15300"
     CDS             446..1747
                     /locus_tag="VGB85_15300"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0135449.1"
                     /translation="MTEETPTPTARIKRIEVVGLFGLYDHVIDLNLEDRVTILHGPNG
                     VGKTVLLHMVRSILGSDFEKLAQIPFQRFRVDFTSGEPIDIHVEHKALEMEFPRRARA
                     QQIKTLRNESPSGTYTCNYSPHTSYPPSDPQDDGGLIRQFRETVWLPNRSETALWEDL
                     GDRVPAHLIEAQRILRPLEKAYGIVDTASRLHPAVWGDAIDLQRRIADLLRKYGTVAQ
                     DLDQSFPTRLLQRTGQSLPVQVLKSRMVRLEELRAALQSNGILDERRAKPFNVSSLDG
                     LTTAQSDVMALYVEDNEAKLGIFVELFQRVELLLRIVNGRFRNKTLRADHRQGLIVVD
                     HRGQPLELRALSSGEQQQIVLVYDLLFRVPPNTLVLIDEPELSLHVLWQKRFLPDLLE
                     IVKVAGIDALIATHSPFIVGDRSDLMVALDAEIDHDEPLQP"
     gene            1725..2597
                     /locus_tag="VGB85_15305"
     CDS             1725..2597
                     /locus_tag="VGB85_15305"
                     /inference="COORDINATES: protein motif:HMM:NF025844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4435 domain-containing protein"
                     /protein_id="HEY0135450.1"
                     /translation="MTSLSSLKNSGELFAELLMSRTGFDGSFLVVEGDDDSRFWRRKV
                     AKGKCDLVIAGGRLNVEGLITRVDTHRFGGTLGLVDADFSGIDGTSPSPNLIYTEHHD
                     LEALLLQSSALDAVLVEYGDKARIEAFEARSGQRVVDALLARGLPYGRLRWLSQRLSL
                     CLNFTKIPPARFIGSDWHFDVDGLHDVAASLCNLPRADLLAHLAALPDTSPWMLCQGH
                     DLLHILNLGLGTALGRHYPGHDALGKLLRQAFQLHEFQASPLYRDIRSWEARNPPYLV
                     LAPDPASPIGQPTP"
     gene            3061..>3495
                     /locus_tag="VGB85_15310"
     CDS             3061..>3495
                     /locus_tag="VGB85_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY0135451.1"
                     /translation="MTALAANSPRVLSGIQPSGIIHLGNYFGAIRQHIALQNTYPDQA
                     LYFIADYHALTTLRDAEALRQNVFDSAVTYLALGLDPGRAMLFRQSDVPEVTELAWLL
                     ASVTGMGLLERAHSYKDKLANNITPSVGLFFYPVLMAADILAY"
BASE COUNT          633 a         1314 c          941 g          607 t
ORIGIN      
        1 cgacgcccgc gaggtcgagt tctccgtgct cggcaacggc gacgagtcga cgatcgtcag
       61 cggccccggc gagatcggcc tgccgcccgg cacctggtac gactacgaca acaagtacat
      121 caaggacgtc gccaccctgc agatccccgc cgccctgccc gacgagctca ccgcccgcat
      181 gcaggacatc tgcctccgcg ccttccgcct gaccggttgc aagggcctcg cccgcgtcga
      241 cttcctcgtc gaccgcacca ccctcgagcc ctacctcaac gagatcaaca cgatgccggg
      301 cttcacctcg atcagcatgt accccaagct gctcggccac atcggcctcg gctacaccga
      361 agtcctcacc cgcctctgcg acctcgccct cacccaccac gccgaccggc aaaaactcag
      421 cgtcacccgc tgacggaccc acgccatgac cgaagagacc ccgaccccca ccgcccgcat
      481 caagcgcatc gaagtcgtcg gcctcttcgg tctctacgat cacgtcatcg acctcaacct
      541 cgaagaccgc gtcacgatcc tgcacggccc gaacggcgtc ggcaagaccg tgctcctgca
      601 catggtccga tctattctgg gcagcgactt cgagaagctc gctcagatcc ccttccagcg
      661 ctttcgcgtc gacttcacgt ccggcgaacc catcgacatc cacgtcgaac ataaggcgct
      721 cgaaatggag tttccgcgaa gagcgcgtgc gcaacagatc aagacgctgc gaaacgaatc
      781 accatccggc acttacacat gcaactattc accgcacacg tcctatcctc cctctgaccc
      841 acaggacgac ggtggattaa ttcgccagtt tcgagagacc gtctggcttc ccaatcggtc
      901 agaaaccgcc ctctgggagg atctcgggga tcgagttccc gcgcacctta tcgaggcaca
      961 gcgaatcctt cgccctctcg aaaaggcata tgggatcgtc gacacggcct caagacttca
     1021 cccggcagtg tggggcgacg ccattgatct ccagcgccgc attgcagatc tgctccgaaa
     1081 gtacggcaca gttgcccaag acctcgacca gtccttcccg actcggctgc tgcaacgcac
     1141 aggtcagagc ctgcccgtgc aagtcctcaa gtctcgcatg gtgaggctcg aggaactcag
     1201 ggccgcgttg caaagcaacg gcattcttga tgagcgtcgc gccaaaccct tcaacgtctc
     1261 atcccttgac ggtttgacga ccgcccaaag cgacgtcatg gccctgtatg tcgaggacaa
     1321 cgaggccaag ctcggcatct tcgtcgaact tttccaacgc gtcgagctgt tgttgagaat
     1381 cgtcaacggt cgcttccgta acaagaccct ccgcgccgat caccgccaag gtctcatcgt
     1441 tgtagaccac cgcggacaac cgctcgaatt gcgtgccctt tcgtccggtg agcagcagca
     1501 gatcgtgctc gtgtacgacc tcctctttcg cgtccccccg aacaccctcg tcctgatcga
     1561 cgagcccgag ctctcgctgc acgtcctctg gcaaaagcgc tttctccccg acctgctcga
     1621 gatcgtcaag gtcgccggca tcgacgcctt gatcgccacg cactcgcctt tcatcgtcgg
     1681 cgaccgctcg gacctgatgg tcgcgctcga cgcggagatc gatcatgacg agcctctcca
     1741 gccttaagaa ttctggagag ctgttcgccg agctgctcat gagccgtacc ggcttcgacg
     1801 gtagcttttt ggtcgtcgag ggcgacgatg acagtcgctt ttggcgccgc aaagtcgcca
     1861 aaggtaagtg cgacctcgtt atcgctggtg gccgcctcaa tgtcgaaggc cttatcacca
     1921 gggtcgacac ccaccgcttc ggcggcaccc tgggcctcgt cgacgcggac ttcagcggca
     1981 tcgacgggac cagcccgtct cccaacctca tctacaccga gcaccacgac ctcgaagctt
     2041 tgttgcttca gagctccgcg ctcgacgccg tcctggtcga gtacggcgac aaagcccgca
     2101 tcgaagcctt cgaggcccgc agcggacaac gcgtcgttga cgcattactg gcccgtggcc
     2161 tgccttatgg ccgcctgcgc tggctctccc agcgactctc gctgtgcctg aacttcacca
     2221 agatcccgcc cgcccggttc atcggttccg actggcattt cgatgtggac gggctccacg
     2281 acgtcgccgc gagcctttgc aacctgccca gagccgacct cctggctcac ctcgcagcgc
     2341 tgcctgatac ctcaccatgg atgctatgtc aggggcacga cctgcttcac atcctcaacc
     2401 tgggcctggg cactgcgctc ggccgtcact atccgggcca cgacgcgctc ggcaagctgc
     2461 tccgccaggc cttccagctt cacgagttcc aggcatcccc gctgtaccgc gacattcgta
     2521 gctgggaggc gcggaaccct ccttatctcg tcctcgcgcc cgatccggcc tccccgatag
     2581 gacagcccac gccgtgagct cggggggcat gcagtccggt cgcgcgacgt cgtggaccct
     2641 cgccacgagc ggggcgccga cgatgcgcac tccgggggaa gggccgactg gcgggggaag
     2701 cagcgggagg acccagggct cgcgcgcaga cgccccctcg tccccccgac gactcgaccg
     2761 aggggtcggt gacagagctc tggcgatctc gcgagatggg gcggcgagca cgtgctctgg
     2821 gaggacccgc gtcgcaggga cccgcagccc gtcggccccc ccggcccata ccgcctcgcc
     2881 ccgcgtcgcc ccgaacacgc taaatctggc cgccgcccct cccaagtaac ccgaacacgc
     2941 tccccaggcc tccgcgccgc ccgaacacgc cagatcagcg cccctccgcg ccgcccgaac
     3001 acgcccggct cgccgagccc gacattctcc gcccacgcca ccgaacagaa gtaaccgacc
     3061 atgaccgccc tcgccgcgaa ttccccccgg gtgctctccg gcatccagcc ttcgggcatc
     3121 atccacctcg gcaattactt cggcgcgatc cgccagcaca tcgccctgca aaatacctac
     3181 cctgatcagg cgctctactt catcgccgac tatcacgcgc tgaccaccct gcgcgacgcc
     3241 gaggcgctgc ggcagaacgt cttcgactcc gcggtcacct atctcgcgct cggcctcgat
     3301 cctgggcgtg cgatgttgtt tcgccagtcg gacgtcccgg aggtcaccga gctggcgtgg
     3361 ttgctcgctt cggtcaccgg catgggtttg ctcgagcggg cgcacagcta caaggacaag
     3421 ctcgccaaca acatcacgcc gtccgtcggg ctgttctttt acccggtgct gatggccgcc
     3481 gacatcctcg cgtac
//
LOCUS       DASWNJ010000901         3717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_51921, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000901 DASWNJ010000000
VERSION     DASWNJ010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51921"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VGB85_15315"
     CDS             complement(<1..404)
                     /locus_tag="VGB85_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135452.1"
                     /translation="MLSTITLLASTLAFSPALSLAPVMATSGYNPGDMGLDCGDDDGD
                     IDIDLDLDACLDLDINALLYCKVKVGAKCHAACEVNVDAYCDAQADADEWDAQAHAQC
                     EAEVEAQCHANCDIDGSVFCKADIYVGADICL"
     gene            complement(958..1614)
                     /locus_tag="VGB85_15320"
     CDS             complement(958..1614)
                     /locus_tag="VGB85_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135453.1"
                     /translation="MAYLERLEAMAAELARHPLIEVLEHAVGSPYQADPVIARRHEAY
                     IETQLRDRPLMQEARRDPSLRAFYKELGWYRLVWRLHRDRLDPARHAWAFACEDGDWQ
                     LEGHINILEEVDVFEPQEGMIWFAEDPPEDPLRTIYPLDRPHVQRATVLVPGPTGLEL
                     ALYLPASRDLGSVRLRFDAWFDRLLATRGMVDTMWSSPGPDFERLRSVFPDVDPRPFA
                     "
     gene            complement(1727..2155)
                     /locus_tag="VGB85_15325"
     CDS             complement(1727..2155)
                     /locus_tag="VGB85_15325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135454.1"
                     /translation="MLSTITLLASTLAFSPALSVAPVMATSGYNPGGIGISIGGGGGG
                     GDIDVDLDACLDLNLNAVLECKVKVGAQCHAACEVNVDAYCDAQVDADEWDAKAHAQC
                     EAEVEAQCHANCDIDGSVFCKADIYVGADICLDIGLDLDL"
     gene            complement(2667..3380)
                     /locus_tag="VGB85_15330"
     CDS             complement(2667..3380)
                     /locus_tag="VGB85_15330"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0135455.1"
                     /translation="MPHSTIDLTGRHVIITGGAGALGTAVVARFLAAGATCHIPTIDD
                     GPGSLGLPGARYIPRIDLGAEQTVVDLYAGLPELWASVHLAGGFTWNRLEDTKSADVR
                     AQFEMNAMTCFLCSREAVKRMRASTPDSEVGGRIVNVGSRATLVPGAGVSAYAMAKAA
                     VQALTTQLAEELRSEQILVNAVLPSIIDTPANRASMPDAEFDRWPKPAEIAEAILFLA
                     SPSNSLTSGALVPVYGRGV"
     gene            complement(3409..>3717)
                     /locus_tag="VGB85_15335"
     CDS             complement(3409..>3717)
                     /locus_tag="VGB85_15335"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEY0135456.1"
                     /translation="PIALVSDLEGAVEHLQRAGVAILAATVGPHARPLDTVRPPPRRL
                     LMVGNEGAGLPPALLARADLELTIPIAAEVDSLGVAAATAVLLHALRTGANSSRPDGI
                     "
BASE COUNT          589 a         1067 c         1498 g          563 t
ORIGIN      
        1 tcgaggcaga tgtcggcgcc gacgtagatg tcggccttgc agaagacgga gccgtcgatg
       61 tcgcagttgg cgtggcactg ggcctcgacc tcggcctcgc actgggcatg ggcctgggcg
      121 tcccactcgt cggcgtcggc ctgggcgtcg cagtaggcgt cgacgttgac ctcgcaggcg
      181 gcgtggcact tggcgccgac cttgaccttg cagtagagga gggcgttgat gtcgaggtcg
      241 agacaggcgt cgaggtcgag gtcgatgtcg atgtcaccgt cgtcgtcacc acagtcgaga
      301 cccatgtcgc ccgggttgta gccggaggtc gccatgacgg gggcgaggga gagggcgggg
      361 gagaaggcga gggtggaggc gagcagggtg atcgtggaaa gcatggcaga ggtccttttc
      421 ttcgaggggt gtcgaaggag cgcgtcgtgt cttgatggcc gccgggagat tccgtcgcgg
      481 ctcgatgagt cgagtatgcg ggccggagat tttgcggcca caagcgccag cgagacaatg
      541 tgcgcacagc gagcagcgcg gcgcggtgga ttggccaatg gcggggttac agagggagtt
      601 gcggccaaat ttcaaaaaat agaggggcgc tgcgaggggc gtggaggctg ctgaaacggg
      661 cgcaaaatgg ccgggtttca aaacgcatcg tgtgtcgatc gtgccgcagg accgacgagg
      721 agaacatcgc ataacgagcg cgggacgggg cgcggaagac ttgctcgttt tctggtcaag
      781 ttgttgaaat gaaacggttt ttctcgacgt ccggcggcgc gaaccgcggg cctggaagcg
      841 gcgacggggc gggtgtcgat gaaatgcgac agccacgggg tgtgcacgtg tgcatcgacg
      901 tcaggttcat cagggccgcc agcgcgagat gtgacgctcg cgtggaggag ccgggtctca
      961 ggcgaagggc cgagggtcga cgtcggggaa tacggagcgc aggcgctcga agtcggggcc
     1021 ggggctcgac cacatggtgt cgaccatgcc gcgcgtggcg agcagccggt cgaaccaggc
     1081 gtcgaagcgc agccggacgc tgccgaggtc gcgggaggcg ggcaggtaga gggcgagctc
     1141 gaggccggtc gggccgggga cgaggacggt cgcgcgctgg acgtggggcc ggtcgagcgg
     1201 gtagatcgtc cgcaaggggt cctcgggcgg gtcttccgcg aaccagatca tgccctcctg
     1261 cggctcgaag acgtcgacct cctcgaggat gttgatgtgg ccctcgagct gccaatcgcc
     1321 gtcctcgcac gcgaacgccc aggcgtgccg cgccgggtcg aggcgatcgc ggtgcagccg
     1381 ccagaccagc cggtaccagc cgagctcttt ataaaaagcc cgcagcgagg ggtcgcggcg
     1441 ggcctcctgc atcagggggc ggtccctcag ctgggtctcg atgtatgcct cgtggcggcg
     1501 agcgatcacc gggtcggcct ggtacggcga gccgaccgcg tgctcgagca cctcaattaa
     1561 tgggtgacgg gcgagctccg cggccatcgc ctcgagtcgt tccaggtagg ccatggcgac
     1621 cgagggtatc agcgcggccg gcggaggtcg tttttttata aaacgccgac aggcgcccac
     1681 gcagatgcgg ggcgcccggg gcagggtgag cggggctcac cggagattac aggtcgaggt
     1741 cgaggccgat gtcgaggcag atgtcggcgc cgacgtagat gtcggccttg cagaagacgg
     1801 agccgtcgat gtcgcagttg gcgtggcact gggcctcgac ctcggcctcg cactgggcgt
     1861 gggccttggc gtcccactcg tcggcgtcga cctgggcgtc gcagtaggcg tcgacgttga
     1921 cctcgcaggc ggcgtggcac tgcgcgccga ccttgacctt gcactcgagc acggcgttga
     1981 ggttgaggtc gagacaggcg tcgaggtcga catcgatgtc gccaccgcca ccaccgccgc
     2041 cgatgctgat gccgatgcca ccggggttgt agccggaggt ggccatgacg ggggcgacgg
     2101 agagggcggg ggagaaggcg agggtggagg cgagcagggt gatggtggag agcatgggtg
     2161 tgggcctttt tccccgaaca tcgtcggggc agtgagtgtc ttgggggccg ccgggagatt
     2221 ccgtcgcggc tcgatgaacc gagtatgcgg gcggaccgat ccgcggccag ttcgacgagg
     2281 gaatgcgacg cggcgagggc cgcggcgcgg cgcggcagat cggcgctgcg agggctcagg
     2341 aggccgattc acggcgatcc gacgatcttc gtcgggccgc gacggcgcgc gcgaatgctg
     2401 ccacaacgcg agtttttgcc cgcattgcaa agctcaaggt gtgtcgattc tgcggcagcc
     2461 ggggccggcg aaacatcgca tatcgaatgc ggcgacggac ggcgcgcgac cgggcagacg
     2521 ccgcaagtgc ccgtcattcc acggttttga tcgggtgggg gcggcgcgcc ccgcgggatt
     2581 ttttcggcga tcgggccggg tgtcgagcaa atgcgacacc acggcggcga cgctgtgggg
     2641 cttactggca gatacttcga cttacatcag acgccgcggc cgtacacggg cacgagcgcg
     2701 ccggaggtga ggctgttgct cgggctggcg aggaacagga tcgcctcggc gatctcggcg
     2761 ggcttgggcc agcggtcgaa ttcggcgtcg ggcatgctgg cgcggttggc cggcgtgtcg
     2821 atgatcgagg gcagcacggc gttgacgagg atctgctcgc tgcgcagctc ctcggcgagc
     2881 tgggtcgtca gcgcctgcac ggcggccttg gccatcgcat aggcggacac gccggcgccc
     2941 ggcacgaggg tcgcgcgcga gccgacgttg acgatccggc cgccgacctc cgagtcgggg
     3001 gtgctggcgc gcatgcgctt gacggcctcg cgtgagcaca ggaagcaggt catcgcgttc
     3061 atctcgaact gggcccggac gtcggcggac ttggtgtcct cgaggcggtt ccaggtgaaa
     3121 ccgccggcga ggtgcacgga ggcccacagc tcgggcaggc cggcgtagag gtcgacgacg
     3181 gtctgctcgg cgccgaggtc gatgcggggg atgtagcgcg cgccgggcag gccgagcgag
     3241 ccggggccgt cgtcgatcgt cgggatgtgg caggtcgcgc cggcggcgag gaagcgggcg
     3301 acgacggcgg tgccgagggc accggcgccg ccggtgatga tgacgtggcg gcccgtgagg
     3361 tcgatggtgg aatggggcat gacggggtcc agtgagcgag ggggtccgtc atataccatc
     3421 cggacgcgac gaattcgcgc cggttcgcag ggcgtgcagc agcaccgcgg tggccgcggc
     3481 gacgccgagc gagtcgacct ccgctgcgat cgggatcgtc agctcgaggt cggcgcgggc
     3541 gagcagcgcg gggggcaggc cagcgccctc gttgccgacc atcagcagcc ggcgcggcgg
     3601 cgggcggacg gtgtcgaggg gacgcgcgtg cgggccgacg gtcgcggcga ggatcgccac
     3661 gccggcgcgc tgaaggtgtt cgacggcgcc ttcgaggtcg gagacgagcg cgatcgg
//
LOCUS       DASWNJ010000902         1548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_547476, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000902 DASWNJ010000000
VERSION     DASWNJ010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547476"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VGB85_15340"
     CDS             <1..790
                     /locus_tag="VGB85_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135457.1"
                     /translation="AIAHVRAAEALMRSSCEVGAAEGLCVELTTDKTLGSVRARDKDQ
                     LAQARGQLQAARGQLAAAGTAPDKLDPPLAVAPGELAAARRTLSLLDGDLGAEAALLI
                     QPPSKYEPGRSLLWYQRRVAEVERMQLVYEGVNPGAGREVVIGARDVESIAHDRFAAV
                     IEARKAQVFEADIGLLEQVASAVAARSPGSSEAAALSTTMQRMANQRRGEAFTCYQRC
                     VELVARWGDDPEGRATACRAGLGRLVQRYEARLEYAPDWRGRLR"
     gene            complement(809..>1548)
                     /locus_tag="VGB85_15345"
     CDS             complement(809..>1548)
                     /locus_tag="VGB85_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135458.1"
                     /translation="LIHVAAFETSASDTDSDASRTHFQAALAVAPEDPYALCVALRLY
                     LALAARAPERLALAAQLCSERLPASAEGDGGDPRGAALLAATCGRVALEARDPGEARR
                     RFALALRIDPADAAARLAWAAAELAAGNDAAAAELFAGAVRAPGTNERYLAGMGLGVA
                     RLRLHDPAAAEAGYRIAAAAHGWHTGDPLDRLPPELLFNLGTLLAQLPDRARRAEART
                     LLTAYTEHPAADELRRLRCRQLLLELQ"
BASE COUNT          180 a          541 c          664 g          163 t
ORIGIN      
        1 ggcgatcgcc catgtgcgcg cggccgaggc gttgatgcgc agctcgtgtg aggtcggcgc
       61 ggccgagggc ctgtgcgtcg agctgacgac ggacaagacg ctcggcagcg tgcgcgcgcg
      121 cgacaaggac cagctggcgc aggcgcgcgg gcagctgcag gccgcgcgcg ggcagctggc
      181 ggcggccggc acggcgcccg acaagctcga tccgccgctg gcggtcgcgc ccggcgagct
      241 ggcggcggcg cggcggaccc tgagcctgct cgacggggac ctcggcgccg aggcggcgct
      301 gctgatccag ccgccgagca agtacgagcc ggggcgctcg ctcctgtggt accagcgccg
      361 cgtcgccgag gtcgagcgca tgcagctggt gtacgagggc gtcaaccccg gggccggtcg
      421 tgaggtcgtg atcggcgcgc gcgacgtcga gtcgatcgca cacgaccgct tcgccgcggt
      481 gatcgaggcc cgcaaggcgc aggtcttcga ggccgacatc ggcctgctcg agcaggtcgc
      541 gtccgccgtc gcggcgcgca gccccgggag cagcgaggcc gcggcgctgt cgacgacgat
      601 gcagcgcatg gccaaccagc ggcgcggcga ggcgttcacc tgctatcagc gctgcgtcga
      661 gctggtcgcc cgctggggcg acgatccgga gggccgcgcg acggcctgcc gcgcgggcct
      721 cggacgcctc gtgcagcgct acgaggcccg cctcgagtac gcgcccgact ggcgcggccg
      781 cctgcgctga gcgaccacag gacgcgcgtc actgcagctc gagcagcagc tggcggcaac
      841 gcaggcgccg cagctcgtcg gccgcggggt gctcggtgta cgcggtcagc agggtgcggg
      901 cctccgcgcg gcgggcccgg tcggggagct gcgcgagcaa ggtgccgagg ttgaacagca
      961 gctcgggcgg caggcggtcg agcgggtcgc cggtgtgcca gccgtgcgcc gcggcggcga
     1021 tgcgatagcc cgcctcggcg gcggccgggt cgtgcaggcg caggcgcgcg acgccgaggc
     1081 ccatgccggc gaggtagcgc tcgttggtgc cgggggcccg cacggcgccc gcgaacagct
     1141 ccgccgcggc ggcgtcgttg ccggccgcga gctcggcggc ggcccaggcc aggcgcgcgg
     1201 cggcgtcggc ggggtcgatc cgcagggcga gcgcgaagcg gcggcgggcc tcgccggggt
     1261 cgcgggcctc gagcgcgacg cggccgcagg tggcggccag caaggcggcg ccgcgcgggt
     1321 cgccgccgtc accctcggcg ctggcgggca gccgctcgct gcacagctgc gcggcgaggg
     1381 cgagccgctc gggcgcgcgg gcggcgagcg cgaggtacag gcgcagcgcg acgcacagcg
     1441 cgtaggggtc ctcgggcgcg acggcgaggg cggcctggaa gtgggtgcgc gaggcgtcgc
     1501 tgtcggtgtc gctggcgctg gtctcgaagg cggcgacgtg gatcagcg
//
LOCUS       DASWNJ010000903         4068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_612871, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000903 DASWNJ010000000
VERSION     DASWNJ010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612871"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VGB85_15350"
     CDS             complement(<1..334)
                     /locus_tag="VGB85_15350"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0135459.1"
                     /translation="MALGSDWLGRRARLSPDALAVLDAADGRAWTFAEWDPPVCRTAR
                     LLQALGVGRGDRVAVLARNSVAFLELWLACGKLGAVLQPFNWRLTASELQALLADGDP
                     AVLVHGPEF"
     gene            complement(431..1990)
                     /locus_tag="VGB85_15355"
     CDS             complement(431..1990)
                     /locus_tag="VGB85_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135460.1"
                     /translation="MRTPAPLAPVLLALFTACGDDAGDSTTQASATITSASATDSSAS
                     SGDVTPTTGDSGATEAQTTDVTSTTDATTDATTDTTITDTTTDATSDTTTDATTDATT
                     DTGGLLDGLELQETFEQGVLNGWEKVERWKDVKNAGFIQEDRFSLDMRAAQNGPRLTY
                     FGEDPVPHSSVFIVYYAPGWDAAKQATPVLLIHGADQADRAWANPGELGDYGCGQKTC
                     PGTGLMQDLVAQGHSVFAVSFAHVHGDNFFWAEQIHNAIRIIRERTGAAKVDLIAWSK
                     GTMPARMYTSSLTQPWSTPYQDDVRKVILIGGPNLGMDYSFRYGNALNGTVAIDYGGK
                     NHAPLPHDELYINFQWVNRAEYGIYKSAKGDNFRGQLQSLARWDAKYPLTGVANFGLG
                     EFTVGDSESTYVGEAKAKVKGVYARGKGIDAAIAQGSIIDDIIAAGMPAGVETYLLCG
                     DIDLKDSSKMMTGVPNEIAGPSDAVVFIESCAAEDGIGNLAGTAILPWNHLQLGWHAD
                     AVTQMLTWLGE"
     gene            2232..3329
                     /locus_tag="VGB85_15360"
     CDS             2232..3329
                     /locus_tag="VGB85_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135461.1"
                     /translation="MHHSRRALALLALLVLIPMGGCVKLDSFSCAQSSECQNGDVLGT
                     CELGGVCSFPDAACPSGKKYGELAGEQSGQCVDMPGDTTGAPTTSPTEPATTDASSTT
                     FDPDSSTTFDPTTMTSEVSATTTGATTTTSSTTTTGEPDTTTGGPTCGEVGSACVEGV
                     CCGGCAVCDGDNICRPAPADQKPCGVCSVCGDNGECSFEPADSPCPTDCGAIVWQEMA
                     GMASAACYAYDPMPTDGACDGAGSCMLPDPVVAKCPEPDPKEATPLAKCELLCKEEKN
                     PCIPGGPASAVDMDSYCFVQTPSPGCTTTCSDDKTFLNPASCVDGVCVHTMQDECGSY
                     VCDPEALMCLEACVDSDDCAVGLVCTDGVCA"
     gene            complement(3346..>4068)
                     /locus_tag="VGB85_15365"
     CDS             complement(3346..>4068)
                     /locus_tag="VGB85_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135462.1"
                     /translation="VAVEHTGAPAQGPNPSDGLNNIECYGAARLIVSNARLVRGSSGV
                     YLVECPDAHLSLLEGHDFRGPFPRGQLAQFNHSDGAVLEDFSVINPRASSWPEDNVNV
                     YQSVDVEIRRGMIDGNNAPSGVGVIFDGDFSLGVVEDVDAIRMGNGCFSDYAGSDGVV
                     FRRTRCRENICEDQGRGLPASNALMWAGHPGYTKIRLEDSHYFASCNGNLLWPAESFE
                     VAELAELDFTMRPAIDVQLCWE"
BASE COUNT          577 a         1414 c         1438 g          639 t
ORIGIN      
        1 cgaattccgg cccgtggacg agcaccgccg ggtcgccgtc ggcgagcagc gcttgcagct
       61 cgctcgccgt cagccgccag ttgaacggct gcagcaccgc gccgagcttg ccgcacgcga
      121 gccagagctc gaggaaggcg acgctgttgc gggcgagcac ggcgacccgg tcgccgcggc
      181 cgaccccgag cgcctgcagc aggcgggcgg tgcggcacac gggcgggtcc cactcggcga
      241 aggtccacgc gcgcccgtcg gcggcgtcga ggaccgcgag ggcgtcgggc gagagccgtg
      301 cgcgtcgtcc gagccagtcg ctgccgaggg ccatgtcatc gacatagccc cagcgccgcg
      361 cggctgtcca ctgcatcctg cggcgcgcag gatcgggcgc ctgccctccc cggctgctac
      421 tccccggctg ctactcgccg agccaggtca gcatctgcgt gaccgcgtcg gcgtgccagc
      481 cgagctgaag atggttccac ggcaggatcg ccgtgcccgc gaggttgccg atcccgtcct
      541 cggcggcgca ggactcgatg aacacgacgg cgtcgctcgg cccggcgatc tcgttgggca
      601 cgccggtcat catcttgctg ctgtccttga ggtcgatgtc gccgcacagc aggtaggtct
      661 cgaccccggc cggcatcccg gccgcgatga tgtcgtcgat gatcgacccc tgggcgatcg
      721 ccgcgtcgat gcccttgccg cgcgcgtaca cccccttgac cttggccttg gcctcgccga
      781 cgtaggtcga ctcgctgtcg ccgacggtga actcgccgag gccgaagttg gcgacgccgg
      841 tcagcgggta cttggcgtcc cagcgggcga gcgactgcag ctggccgcgg aagttgtcgc
      901 ccttggcgct cttgtaaatt ccgtactccg cgcggttgac ccactggaag ttgatgtaga
      961 gctcgtcgtg gggcagcggc gcgtggttct tgccgccgta gtcgatcgcc acggtgccgt
     1021 tgagggcgtt gccgtagcga aagctgtagt ccatgccgag gttcgggccg ccgatcagga
     1081 tcaccttgcg cacgtcgtcc tggtacgggg tgctccacgg ctgcgtcagg ctcgacgtgt
     1141 acatgcgcgc gggcatcgta cccttgctcc acgcgatcag gtcgaccttg gccgcgccgg
     1201 tgcgctcgcg gatgatccgg atcgcgttgt ggatctgctc ggcccagaag aagttgtcgc
     1261 cgtgcacgtg cgcgaacgag accgcgaaca cgctgtggcc ctgcgcgacg aggtcctgca
     1321 tcagaccggt gcccgggcag gtcttctggc cgcagccgta gtcgccgagc tcgcccgggt
     1381 tggcccacgc gcggtcggcc tggtcggccc cgtggatcag caggaccggg gtcgcctgct
     1441 tcgccgcgtc ccagccgggc gcgtaataca cgatgaacac gctcgagtgc ggcaccgggt
     1501 cctcgccgaa gtaggtcagc cgcggaccgt tctgggccgc gcgcatgtcg agcgagaagc
     1561 ggtcctcctg aatgaacccc gcgttcttca catccttcca gcgctcgacc ttctcccagc
     1621 cgttgagcac gccctgctcg aaggtctcct gcagctcgag gccgtcgagc agaccgccgg
     1681 tgtcggtggt cgcgtcggtc gtcgcgtccg ttgttgtgtc gctcgtcgcg tccgttgttg
     1741 tgtcggtgat ggttgtgtcg gtggtcgcgt ccgtcgtcgc gtcggtcgtg ctggtcacgt
     1801 cggtggtctg cgcctccgtc gcgccgctgt ccccggtggt cggcgtcacg tcaccgctgc
     1861 tcgccgaact gtcggtcgcg ctcgcgctgg tgatggtggc cgaggcctgc gtggtcgagt
     1921 caccggcgtc gtcgccacag gcggtgaaca gggccagcag gacgggtgcg aggggcgcgg
     1981 gagttcgcat atgcgccagc atacccgggc agccgacgcc gcgcgaagct gcgcgcgtga
     2041 gctttgtgtg cggtgcaaca tgccggcgac cacatccgcc gatagtggcc gccaggcccc
     2101 gggcactggc cgtactcacg cctcgcgaga ttgtgcagcg caacgtagaa ggcccgcgac
     2161 agatcgcgcc tggggtctcg tgacgcgccc ttgctggccg cgacgcgggc gtgccaagct
     2221 gcgccccggt gatgcaccac tcccgccgtg ccctcgccct gctcgccctg ctcgtcctga
     2281 tccccatggg cggctgcgtg aagctcgaca gcttcagctg cgcgcagagc tcggagtgtc
     2341 aaaacggcga cgtgctcggc acctgcgagc tcggcggcgt gtgcagcttc cccgacgcgg
     2401 cgtgcccctc gggtaaaaaa tatggcgagc tcgccggcga gcagtccggc cagtgcgtcg
     2461 acatgcccgg ggacacgacc ggcgcgccga cgacctcgcc gacggagccg gcgaccaccg
     2521 acgcgtcctc gacgaccttc gacccggact cctcgacgac cttcgatccg accacgatga
     2581 cctccgaggt cagcgcgacg acgacgggcg cgacgacgac gacgagctcg acgacgacga
     2641 ccggcgagcc cgacacgacc accggcgggc cgacctgcgg cgaggtcggg tccgcctgcg
     2701 tcgaaggggt gtgctgcggc ggctgtgcgg tctgtgacgg cgacaatatc tgcaggcccg
     2761 cgcccgcgga tcagaagccg tgtggcgtgt gctcggtctg cggcgacaac ggcgagtgca
     2821 gcttcgagcc ggccgacagc ccctgcccca ccgactgtgg cgccatcgtc tggcaggaga
     2881 tggccgggat ggcgagcgcc gcctgttacg cgtacgaccc gatgccgacc gatggcgcgt
     2941 gcgacggcgc gggcagctgc atgctgccgg acccggtcgt ggcgaagtgc cccgagcccg
     3001 accccaagga ggcgaccccg ctcgccaaat gcgagctgct ctgcaaggag gagaagaacc
     3061 catgcatccc cggcggaccg gcgagcgcgg tcgacatgga ctcctactgc ttcgtgcaga
     3121 ccccgtcccc gggctgcacg accacgtgca gcgacgacaa gaccttcctc aaccccgcga
     3181 gctgcgtcga cggcgtctgc gtgcacacga tgcaggacga gtgcggctcc tacgtctgcg
     3241 acccggaggc gctgatgtgc ctcgaggcgt gcgtcgacag cgacgactgc gctgtcgggc
     3301 tcgtgtgcac ggacggcgtg tgcgcgtagc ggggcgcgcc gcggctcact cccagcacag
     3361 ctggacgtcg atcgccgggc gcatcgtgaa gtcgagctcg gcgagctcgg cgacctcgaa
     3421 cgactcggcc ggccacagca agttgccgtt gcacgacgcg aagtagtgcg agtcctcgag
     3481 gcggatcttg gtgtagccgg ggtggccggc ccacatcagg gcgttggagg caggcaggcc
     3541 gcggccctgg tcctcgcaga tgttctcgcg gcagcgggtg cggcggaaca ccacgccgtc
     3601 gctgccggcg tagtccgaga agcagccgtt gcccatgcgg atcgcgtcga cgtcctcgac
     3661 gacgccgagt gagaagtcgc cgtcgaagat cacgccgacg ccggacggcg cgttgttgcc
     3721 atcgatcatg ccgcgccgga tctcgacatc gacggactga tacacgttga cgttgtcctc
     3781 cggccacgag ctggcccgcg ggttgatcac cgagaagtcc tcgagcacgg ccccgtccga
     3841 gtgattgaac tgcgcgagct ggccgcgcgg gaacggcccg cgaaagtcgt ggccctcgag
     3901 caagctcagg tgagcgtcgg ggcactcgac caggtagacc ccgctcgagc cgcggaccag
     3961 ccgggcgttg ctgacgatca ggcgagccgc cccgtagcac tcgatgttgt tgaggccgtc
     4021 cgaggggttc gggccctgcg ccggcgcccc cgtgtgctcg acggcgac
//
LOCUS       DASWNJ010000904         1985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_581700, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000904 DASWNJ010000000
VERSION     DASWNJ010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581700"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1883)
                     /locus_tag="VGB85_15370"
     CDS             complement(<1..1883)
                     /locus_tag="VGB85_15370"
                     /inference="COORDINATES: protein motif:HMM:NF012255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HEY0135463.1"
                     /translation="MAVYETEHDRIVLRVVYDGPGLVGKTTNLEQLAQAFHGDFLPDP
                     PDEAPPAKGQPPANRTRFFDWFAFDGGKLDGHGLAIQVVSVPGHKRFEARRAALLRSA
                     DAIVLVCDSTPAAQESARETLDSQREHLGALASQIPLVVQANKQDLPGALQPQELSEG
                     LGLPLEVPVLAAQASGAIGVRETVVQAVRTAVRRIKRQITRGGIHSLNGTPGDAATLR
                     AELGRLASPALPEDGPKEPPRSRRRPAQRLTIVNEPRVVQMRVANAGKFGTRTPVSEP
                     RDFPTPPEPGRPRARSGEPGDLSARPGLFFDEHPPEAPAHPPTTPPSERVTLPDGDIA
                     TPTLPAEGELSPATLHALVEMATALPPRSTAPAQPAKSDDLSLRTASPPSGPALPKPG
                     SRPPRRIPSAPEIPMVSADLSKKPARRPRTRPQLVVPAAQSISPTNKLVLPPAPRTGP
                     RPAEPASNNVVAPAKLEATPASSAASASAGALAGSASPRAHEPPAATPHAASAGLPAH
                     HPPATTHAAQPPGSSSPPGPAATPYAAQLPATSPREPAATPHAAQLPATSARAPEPAA
                     RPSASASPSSPTRPQPLAEPHASDTATLPPLPRPDIPTGHVWPVPRGRDVLRLLVDLP
                     VERL"
BASE COUNT          207 a          614 c          897 g          267 t
ORIGIN      
        1 aggcgctcga cgggcaggtc gacgagcagg cgcaagacgt cgcggccgcg cggcacgggc
       61 cagacgtggc cggtcgggat gtcggggcgc ggcagcggcg ggagcgtggc ggtgtcggag
      121 gcgtgaggct cggcgagcgg ctggggtcgc gtcggcgaag atggcgacgc ggacgcggag
      181 ggccgcgcgg cgggctcggg cgcgcgcgcg ctcgtggcgg ggagttgtgc ggcgtggggc
      241 gtcgcggcgg gctcgcgcgg gctcgtggcg gggagttgtg cggcgtaagg cgtcgcggcc
      301 ggcccgggcg ggctcgagga gccgggaggt tgtgcggcgt gggtcgtcgc tgggggatga
      361 tgcgcgggca ggccggcgga ggcggcgtgt ggagtcgcgg ccgggggctc gtgcgcgcga
      421 gggctcgcgg agccggcgag cgcgcctgcg gaggcgctcg cggcggagct cgcgggcgtc
      481 gcctcgagct tcgcgggggc cacgacgttg ttggacgcgg gctcggccgg cctggggccg
      541 gtgcggggcg cgggtggcag gaccagcttg ttggtcggcg agatcgactg cgcggccggg
      601 acgaccagct gtgggcgcgt gcgtggccgg cgggctggct ttttggacag gtcggcgctg
      661 accatcggga tctccggcgc cgacgggatc cgccgcggcg gtcgggaacc cggcttcggg
      721 agcgcggggc cggacggcgg cgaagctgtc cgcaaggaca ggtcgtccga cttcgcgggc
      781 tgggcgggag cggtcgagcg cggcgggagg gcggtcgcca tctcgacgag cgcgtgcagg
      841 gtcgcgggcg agagctcgcc ctcggccggc agggtcgggg tcgcaatgtc gccgtcgggg
      901 agcgtgacgc gctcgctcgg cggcgtggtg ggcgggtgcg ccggggcctc gggcgggtgc
      961 tcgtcgaaga acagacctgg cctcgcggac aggtcgccgg gttcgccgga cctggcacgc
     1021 gggcgccccg gctcgggggg cgtcgggaag tcgcggggtt cgctgacggg ggtgcgcgtg
     1081 ccgaacttgc cggcgttggc gacccgcatc tggacgacgc gcggctcgtt gacgatcgtc
     1141 aggcgctggg cggggcgccg gcgcgagcgc gggggctcct tggggccgtc ctcgggcagc
     1201 gcgggcgagg cgaggcgccc gagctcggcg cgcagggtcg cggcgtcgcc gggcgtgccg
     1261 ttgagcgagt ggatcccgcc gcgggtgatc tggcgcttga tccttcggac ggcggtgcgc
     1321 accgcctgca cgacggtctc gcgcacaccg atcgcgcccg aggcctgcgc cgcgagcacg
     1381 gggacctcga gcgggaggcc gaggccctcg ctgagctcct gcggctggag ggcgcccggg
     1441 aggtcctgct tgttggcctg cacgacgagc gggatctggc tggcgagcgc gccgaggtgc
     1501 tcgcgctggc tgtcgagggt ctcgcgcgcg gactcctggg ccgcgggcgt gctgtcgcag
     1561 acgagcacga tcgcgtcggc cgagcgcagc agggcggcgc ggcgggcctc gaagcgcttg
     1621 tgaccgggca cgctgacgac ctgaatcgcg aggccgtggc cgtcgagctt gccgccgtcg
     1681 aacgcgaacc agtcgaagaa gcgggtccgg ttggcgggcg gttgaccctt cgccggcggc
     1741 gcctcgtcgg gcgggtcggg caggaagtcg ccgtgaaagg cctgggcgag ctgctcgagg
     1801 ttggtggtct tgccgaccag ccccggcccg tcgtagacga cgcgaaggac gatgcggtcg
     1861 tgctcggtct cgtagactgc catggtcgcc ccctagcggt gtttcatgtc cacgcgcgag
     1921 agctccgagc ggagcaggtt cgatcgcagc agggcccagc ccatgccgat gttgatcgtc
     1981 ttgcg
//
LOCUS       DASWNJ010000905         2992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_547531, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000905 DASWNJ010000000
VERSION     DASWNJ010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547531"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VGB85_15375"
     CDS             <1..274
                     /locus_tag="VGB85_15375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135464.1"
                     /translation="LADLADLRDTGPAVRPASRPMPAAPVPADESGDSDEPEEVDELD
                     ELDSVDDEPAPMTRSAPPPPKKAPPPPPSNLFKPRPLLPIVPPKKK"
     gene            279..1427
                     /locus_tag="VGB85_15380"
     CDS             279..1427
                     /locus_tag="VGB85_15380"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY0135465.1"
                     /translation="MTVPVLKTRWLLTALLACGPAPAPTPVAAPTPAPAPAPVAPAPT
                     PDAAAPPVRKDGTIYAESYHMGTRFSINLWVGDQSASAAGEAIEAAYAEVERIEQIMS
                     EWRPTSELSQLNDAAGGAMRPLSPELFEVLQRSKQISEATDGAFDPTFHGVGQLWKFE
                     PGATPPTREAIAAKLPLVDWRAIELDPNTSSGRLAKPGMKVGLGAIAKGYGVDRASQL
                     LKQRGFMHHIVEGGGDTYVAGTKGGASWKVGIQRPDGPGSLAAIPASDRAIVTSGGYQ
                     RFIEVDGKRYAHIIDPRTGWPIDEASSAQSVTVLAPDATDADAYATAISVMGPVKGLA
                     YVEAHPALEAVLVTRTGEMLVSKDLQATLIVPKAAPPAAGTVKPSAQR"
     gene            1529..>2992
                     /locus_tag="VGB85_15385"
     CDS             1529..>2992
                     /locus_tag="VGB85_15385"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0135466.1"
                     /translation="MAQDRGHDDDMEDDTRDDVKATRVAFAPVLVGRPASTTAPSAPP
                     LRPRALQEQPATGHGDTLPELERAPEKQHTIVAPRFGGTGPVALRGSPTPAVERTRLR
                     LVAGKVVPGTRYRLIRWLGEGGMGVVYEAEHIDIERHVALKILRFDLSQQPEMTQVFR
                     DEARAASRMGHGNIVEVFDFGELPDGRLFFCMELLAGKDLVPVDGATRPAAEVLGILR
                     QLCKGLAAAHAQGIVHRDIKPENIILVSRGGRDGVVKLVDFGISAMLAAGAGAGETSR
                     ISGTPHYMAPEQVQGQPFDGRLDMYAVGCVAYELLTGAPPFPGEVVEEVLLAQLDQPP
                     TPPTQTPHGAAIPPTLEAVILRCLAKKPADRYADMNDLEAALCEAQIAAGLRTDWDDL
                     PLPEVDAERVARLRRGMPSPNAEPTRRRWVVPLSAAVASLAAGVAITFAMLGGPPDEQ
                     ARSEVDRLAEEALLAASLTNYIAAPADDPKAPTAYQKV"
BASE COUNT          444 a         1219 c          957 g          372 t
ORIGIN      
        1 gctcgccgac ctcgccgacc tgcgtgacac cggccccgcc gtccgccccg cgagccgccc
       61 gatgcccgcc gccccggtcc ccgcggacga gtccggcgac agcgacgagc ccgaggaagt
      121 cgacgagctc gacgagctcg acagcgtcga cgacgagccg gccccgatga cccgctccgc
      181 cccgccgccg cccaagaagg ctccgccacc gccgccgagc aacctgttca agccgcgccc
      241 gctgctaccg atcgtgccgc cgaagaagaa gtgaggccgt gaccgtgcct gtcctcaaga
      301 ccaggtggct gctgaccgcg ctgctcgcct gcggcccggc ccccgccccg accccggtcg
      361 ccgcgccgac gcccgcaccc gcgcctgccc ctgtagctcc cgcgccgaca cccgacgccg
      421 cggccccgcc ggtgcgcaag gacggcacga tctacgccga gtcctatcac atgggcacgc
      481 gcttctcgat caacctctgg gtcggcgatc agtcggcgag cgccgcgggt gaggcgatcg
      541 aagccgccta cgccgaggtc gagcgcatcg agcagatcat gagcgagtgg cgtccgacca
      601 gcgagctctc gcagctcaac gacgcggcag gcggcgcgat gcgtccgctc agccccgaac
      661 tattcgaagt gctgcagcgc agcaaacaga tctccgaggc gaccgatggc gccttcgacc
      721 cgaccttcca cggcgtcggc cagctctgga aattcgagcc cggcgcgacc ccaccgaccc
      781 gcgaggcgat cgccgccaag ctgccgctcg tcgactggcg ggcgatcgag ctcgacccga
      841 acacctcgtc ggggcgcctc gccaaacccg gcatgaaggt cggcctcggc gcgatcgcca
      901 agggctacgg ggtcgaccgc gcctcacagc tgctcaagca acgcggcttt atgcatcaca
      961 tcgtcgaggg cggcggcgac acctacgtcg ccggcaccaa gggcggcgcg tcgtggaagg
     1021 tcgggatcca gcgcccggac ggccccggct cgctcgccgc gatccccgcc tccgaccggg
     1081 cgatcgtcac ctccggcggc tatcagcgct tcatcgaggt cgacggcaag cgctacgcgc
     1141 atatcatcga cccacgcacc ggctggccga tcgacgaggc gagcagcgcc cagagcgtca
     1201 ccgtgctcgc ccccgacgcg accgacgcgg acgcctacgc aaccgcgatc tccgtgatgg
     1261 gccccgtcaa gggcctcgcc tacgtcgagg cccaccccgc gctcgaggcc gtgctcgtca
     1321 cccgcaccgg cgagatgctg gtctccaagg atctccaggc gaccttgatc gtgcccaaag
     1381 ccgcgccgcc ggccgctggc accgtgaagc cgtccgcaca gcgctgaagg tcacggtgca
     1441 ggcgttcccc cgcgacgcgg cctagtcacg ccgccgtcac accgtttgca cttgtcgccg
     1501 gatttttctg ctcactacac tgggccccat ggcgcaggac cgcgggcacg acgacgatat
     1561 ggaggacgac acccgcgatg acgtcaaggc gacccgcgtc gccttcgccc ctgtcctggt
     1621 cggccggccc gcctcgacga ccgccccctc ggccccgccg ctgcgcccgc gggccctcca
     1681 ggagcagccg gcgaccggcc atggtgacac gctgcccgag ctcgagcgcg cgcccgagaa
     1741 gcagcacacg atcgtcgcgc cgcgcttcgg cggcaccggg ccggtcgcgc tgcgcggctc
     1801 gccgaccccc gcggtcgagc gcacgcgcct gcgcctggtc gccggcaagg tcgtgcccgg
     1861 cacccgctac cgcctgatcc gctggctcgg cgagggcggc atgggcgtcg tctacgaggc
     1921 cgagcacatc gacatcgagc gccacgtcgc cctcaagatc ctccgcttcg atctcagcca
     1981 gcagcccgag atgacccagg tgttccgcga cgaggcccgc gccgccagtc gcatgggcca
     2041 cggcaacatc gtcgaggtct tcgacttcgg cgagctcccc gacggccgcc tgttcttctg
     2101 catggaactg ctcgccggca aggacctcgt gcccgtcgac ggcgccaccc gccccgccgc
     2161 cgaggtgctc ggcatcctgc gccagctgtg caagggcctc gcagccgcgc acgcgcaggg
     2221 catcgtgcac cgcgacatca agcccgagaa catcatcctc gtcagtcgag gcggccgcga
     2281 cggcgtcgtc aagctggtcg acttcggcat ctcggcgatg ctggccgccg gcgcgggcgc
     2341 gggcgagacc tcgcggatct ccgggacccc gcactacatg gcgcccgagc aggtccaggg
     2401 ccagcccttc gacggccggc tcgacatgta cgccgtcggc tgcgtcgcct acgagctgtt
     2461 gaccggcgcg ccgccgttcc ccggcgaggt cgtcgaggag gtgttgctcg cccagctcga
     2521 tcaacccccg accccgccga cccagacccc gcacggcgcc gccatcccgc cgacgctcga
     2581 ggccgtgatc ctgcgttgcc tcgccaagaa gccggccgac cgctacgccg acatgaacga
     2641 cctcgaggcc gccctgtgcg aggcccagat cgccgccggc ctgcgcaccg actgggacga
     2701 cctcccgctc ccggaggtcg acgccgagcg cgtcgctcgc ctgcgtcgcg gcatgccgag
     2761 ccccaacgcc gagccgaccc gccgccgctg ggtcgtgccc ctctccgccg cggtcgcctc
     2821 gctcgccgcc ggcgtcgcga tcaccttcgc catgctcggc ggcccgcccg acgagcaagc
     2881 gcgcagcgag gtcgaccgcc tcgccgagga ggcgttgttg gccgcctcgc tgaccaacta
     2941 catcgccgcg cccgccgacg accccaaggc gccgaccgcc tatcaaaagg tg
//
LOCUS       DASWNJ010000906         5077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_475382, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000906 DASWNJ010000000
VERSION     DASWNJ010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475382"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1739)
                     /locus_tag="VGB85_15390"
     CDS             complement(54..1739)
                     /locus_tag="VGB85_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135467.1"
                     /translation="MRDLVCIGSCVLLLACGDPGGVAGASASSSGMSEAASTSSTSST
                     STSSTASSSGELPTTGGAPTSSAGGSADATGSDPDTSTGEAEADPWVERRGVLHVHSP
                     YSHDACDGEGLMNGAPNPGCLADLRAAICDGGLDFALLTDHPAHMQEYPFTDVLLYSE
                     AAGDELVMMQDMAWNHMRCADDRRVVLGAGYESHHTLPLGLHHHVDAALYAGLTDATP
                     LADAKALIAGLAAGGAVTAIAHSEESDLSAARIVEVGLDAMEWYNPHGNFKNALGGDN
                     IAGDPGKVLDTIQGLQPFMVGSQSGAHPDLVYLRLLPSWPVEGFNKWREVQRVTAITG
                     LLGSDVHQNVRVAPICDQDNPVLQAACIAAAEAALPEALAGLVAGGTLTMSDGDRLDS
                     FARVLRWLENSVLTEPGPLDLEQLQAALRAGRSYGLFTVFGAPEQLRFAGRTADDTLL
                     WIGDAAVGPVELQLRAPLRPLALGGAPFTTAQADTAELRTELWRTDAGGSVKVADATG
                     LGATIDYMASDPGAYHVEVYVRPQHLREALGSEDELADIEYMWVITNPIRVLR"
     gene            complement(1806..3194)
                     /locus_tag="VGB85_15395"
     CDS             complement(1806..3194)
                     /locus_tag="VGB85_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135468.1"
                     /translation="MKTSLIINCLLLVGGIASVAGCGPKNDGADGGTDTEATAGTGDT
                     TPTGDDTTPTGPGEADCGSCFTVLEGDVSPSGDARLDGVMVAVEQLRRTVLNIQGDFA
                     ADMRALRDIYGLPGSEPTPASVAQLVTAIKEDLAANAQVLRVVHKPAECYSNIEVAAQ
                     ALAQCELNGGCVVAPAGGRPAVQCEGTCIGACAGICDGELSCVETAAAITCDGLCDGT
                     CTSESGLGCNGTCYGQCEGNCALQDSNGNCAGPCDGPCVGTCDAPAPAACPGACTGDC
                     RVEQGSAQCTGKGPCQGQCSFGCEGDCAGRATPLSSVMGCAATKGCEPQAAAQGSASL
                     WCTAPVLDIQYVLSNVMSPEAQGGFITRTATLDRRALAVLQGSAQLGVLITGTSEGKV
                     VFDPPPLVTVTTAIQQVQGLVDGLDIPVGRLPCVAPALQAAVSALADTALSAQATLEA
                     QAVFATFMSNGV"
     gene            complement(3277..>5077)
                     /locus_tag="VGB85_15400"
     CDS             complement(3277..>5077)
                     /locus_tag="VGB85_15400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HEY0135469.1"
                     /translation="LAWGESYVLWSLMAMVRRTDHPMYVDRMARHLDALLQQRDDARG
                     VADYRGVSGACWRNTSYQDKGEPYCYAVHSGMLVYPMAELAAFIAGWRHRDAPAYDGE
                     SFAAKAEVYRAAAVATAAYHDDEWNDAGYYVFRASADFLEFAGKDQPLNQSNAMGLAL
                     LALHDLTGEAVYLERATALAKRMRGMMTASPAGGLVWNYWGGPYAAPGEDISHAALNV
                     EFAVQAAARGVVFTEADLDALATTFLEQVYIDDKTFADHVGGGAVNGDSYRAQIGRWL
                     PLTARRGSVYAAIHDAYQRDYPAADVGSGSTLLGWAYLAAFEPRVCPHFFYPYDWQDG
                     GDAMQATAYGANVLTVPPDLGITCRVPLSVHNSRRTTAAQWDGDKMHRNVEWQPAAEF
                     TARSLAYEPRWPLVYFEEGVLFEFQDEFAPAGAIQVAQPGVHTLPAITSQPPTTGEAG
                     VTLEYAPTGTGDPPFWWSLRAGPAGLAVDPATGVVVFTPASPGAYLFSLGLENDLGEA
                     VQEFTIEVPDAGADSGETPTTGDGTPTTGDGTPTTGDAGSTGDTGDTSDTGSGGPGAT
                     DPAGDAGCGCRGHSGSGLAGLAGLALLLRRRRR"
BASE COUNT          750 a         1868 c         1701 g          758 t
ORIGIN      
        1 gcctccgtgg catcccgcac atgtttgtgg gctcgcgccc ccggcgataa gattcatcgc
       61 aacacgcgga tcgggttggt gatcacccac atgtactcga tgtcggcgag ctcgtcctcg
      121 ctgccgaggg cctcgcgcag atgttgcggg cggacataaa cctcgacgtg atatgcaccc
      181 gggtcgctcg ccatatagtc gatggtcgcg ccgaggcccg tcgcatccgc gaccttgacg
      241 ctgccgccgg cgtcggtgcg ccacagctcc gtgcgcagct cggccgtgtc ggcctgcgcc
      301 gtcgtgaacg gggccccgcc gagcgcgagc ggtcgcagcg gcgcacgcag ctgcaactcg
      361 accggcccga cggcggcgtc cccgatccac aacaatgtgt cgtccgcggt ccggccggcg
      421 aagcgcagct gctcgggcgc gccgaacacg gtaaacaggc catagctgcg accggcccgc
      481 agcgccgcct gcagttgctc gaggtcgagc gggccaggct cggtcaacac gctgttttcg
      541 agccagcgca ggaccctggc gaagctgtcg aggcggtcgc cgtccgacat cgtcagcgtg
      601 ccgccggcga ccagccccgc gagcgcctcc ggcaatgcag cctcggccgc ggcgatacac
      661 gcggcctgca gcaccgggtt gtcctgatcg cagatcggcg cgacgcgaac attttggtgc
      721 acgtcggatc cgagcagccc cgtgatcgcc gtgacgcgct ggacctcgcg ccatttatta
      781 aaaccttcga ccggccacga gggtagcagg cgcagatata cgaggtcggg gtgggccccg
      841 ctctgcgatc cgaccatgaa tggttgcagc ccctggatcg tgtcgagcac cttgccgggg
      901 tcgcccgcga tattgtcgcc gccgagggca tttttaaaat tgccgtgggg gttgtaccac
      961 tccatcgcgt cgagcccgac ctcgacgatc cgcgccgccg acagatccga ctcctcgctg
     1021 tgggcgatcg ccgtgaccgc gccgccggcc gcgagcccgg cgatcagcgc cttggcgtcc
     1081 gccagcgggg tcgcgtcggt caggccggca tacagcgcgg cgtcgacgtg gtggtgcagc
     1141 ccgagcggca gcgtgtggtg agattcatag ccggcgccga gcaccacgcg gcggtcgtcc
     1201 gcgcagcgca tgtggttcca tgccatgtcc tgcatcatca ccagctcgtc gcccgcggcc
     1261 tcgctgtaca ggagcacgtc ggtgaacgga tactcctgca tgtgcgcggg gtggtcggtc
     1321 aacagcgcga agtcgaggcc gccgtcgcag atcgccgcgc gcaggtcggc gaggcagccg
     1381 gggttcggcg cgccattcat cagaccctcg ccgtcgcagg cgtcgtgtga gtacggtgaa
     1441 tgcacgtgca agacgccgcg acgctcgacc cacgggtccg cctccgcctc gccggtgctc
     1501 gtgtcggggt cgctgccggt cgcgtcggcg ctgccacccg cgctcgaggt cggcgcgccg
     1561 ccggtcgtcg gcagctcgcc cgagctgctc gcggtcgacg atgtcgaggt cgatgacgtc
     1621 gacgaagtgc tcgcagcctc cgacatgccc gagctgctcg cgctggcacc cgcgacgccg
     1681 cccgggtcgc cgcaggcgag gagcagcacg caggagccga tgcagacgag gtcgcgcatc
     1741 ttcgcgccat accgcgcgcg gacccgcctt ggcaagcgcg gcccgcatga ccgtgctgcg
     1801 ccgcgctaaa cgccgttcga catgaacgtg gcgaacaccg cctgcgcctc gagcgtcgcc
     1861 tgggccgaca atgccgtgtc cgcgagcgcg gagactgccg cttgcagcgc cggtgcgacg
     1921 cacggcaggc ggcccaccgg gatgtcgagc ccgtcgacga ggccctgcac ctgctggatc
     1981 gccgtcgtca ccgtgaccag cgggggcgga tcgaacacca ccttaccctc gctggtgccg
     2041 gtgatcagca caccgagctg cgccgatccc tgcaagaccg ccagggcgcg gcgatcgagc
     2101 gtcgcggtgc gagtgataaa gccgccctgc gcctccggcg acatcacatt gctcaacaca
     2161 tattgaatgt cgagcaccgg cgccgtgcac cacaggctcg cgctgccctg cgcggcggcc
     2221 tgcggctcgc agcccttggt cgccgcgcag cccatcacgc tgctcagggg cgtcgcccgg
     2281 cctgcacagt cgccctcgca gccgaagctg cactggccct ggcaaggccc cttgcccgta
     2341 cattgcgcgc tgccctgctc gacccggcaa tctccggtgc atgcgccggg gcaggccgct
     2401 ggcgccggcg cgtcgcaggt gccgacgcac gggccatcgc acggcccggc gcaattacca
     2461 ttgctgtctt gcagggcgca attgccctca cactggccat aacacgtacc attgcacccg
     2521 aggcccgact cggacgtgca cgtcccgtca cagagcccat cgcaagtgat cgccgccgcc
     2581 gtctccacgc acgacagctc accgtcgcag atcccggcgc acgcgccgat gcaggtgccc
     2641 tcgcactgca ccgccggccg cccgcccgcc ggcgccacga cgcagccgcc gttgagctcg
     2701 cactgcgcca gcgcctgcgc ggcgacctcg atgttgctgt agcactccgc cggtttgtgc
     2761 acgacccgga gcacctgcgc gttcgcggcg aggtcctcct tgatcgcggt gaccagctgg
     2821 gccacgctcg ccggggtcgg ctcgctcccg ggaagtccgt agatatccct gagcgcgcgc
     2881 atgtccgcgg cgaagtcccc ctggatgttg aggaccgtcc tgcgcagctg ctcgaccgcc
     2941 accatcacac catcgaggcg ggcgtcgccc gacggcgaga catcgccctc gagcacggta
     3001 aagcagctcc cgcagtccgc ctcgcccggg ccggtcggcg tcgtatcgtc gccggtcggc
     3061 gtcgtatcac cggtgcccgc ggtcgcctcg gtgtcggtcc ccccatccgc gccgtcgttc
     3121 ttgggtccgc agcccgcgac cgacgcgatg ccaccgacga gcagcaagca attaatgatt
     3181 aatgaagtct tcatccgccg agtatgcccg cgaacgggcc gcgacgggcc agcaaattct
     3241 cctcaaaggt ccgcgcgggc ctgcgagacc gcgaccttac ctgcgccggc gccgaagcag
     3301 cagcgcgagg ccggcgagcc cggcgagccc ggacccgctg tggccgcggc acccacagcc
     3361 ggcgtcgccc gccggatccg tcgcgcccgg cccgccactg ccggtatcgg acgtatcgcc
     3421 ggtatctccc gtgctgcccg cgtcgcccgt ggtcggcgtt ccatcgcctg tggtcggcgt
     3481 tccatcgccc gtggtcggcg tctcgccgct gtcggccccg gcgtcgggca cctcgatcgt
     3541 gaattcctgc accgcctcgc cgaggtcgtt ctcgaggccg aggctgaaca gataagcccc
     3601 ggggctcgcg ggcgtgaaca cgacgacgcc ggtcgcgggg tcgaccgcga ggccggccgg
     3661 gcccgcgcgc agcgaccacc agaacggcgg atcaccggtg ccggtcggcg catactcgag
     3721 ggtgacgccc gcttcaccgg tcgtcggcgg ctgcgaggtg atcgccggca gcgtgtgcac
     3781 gccgggctgc gcgacctgga tcgcaccggc cggcgcgaat tcgtcctgga actcgaacag
     3841 cacgccctcc tcaaaatata ccagcggcca gcgcggctcg tacgcgaggc tgcgcgccgt
     3901 gaattctgcg gccggctgcc attcgacgtt gcgatgcatc ttgtcgccgt cccactgagc
     3961 cgcggtcgtg cgccgactgt tgtgcaccga taacggaacg cgacaggtga tcccgaggtc
     4021 cggcgggacc gtcagcacat tcgcgccgta cgcggtcgcc tgcatcgcgt cgcccccgtc
     4081 ctgccagtcg tacggataaa agaagtgcgg gcacacgcgc ggctcgaacg cggcgaggta
     4141 ggcccagccg agcagcgtcg agcccgagcc gacgtcggcg gccggatagt cacgctggta
     4201 ggcgtcgtgg atcgcagcgt agacagagcc gcgccgggcg gtcagcggca gccagcggcc
     4261 gatctgcgcg cgatagctgt cgccgttgac cgcgccgccg ccgacgtgat ccgcgaacgt
     4321 tttatcatcg atatagacct gctcgaggaa cgtcgtggcg agcgcgtcga ggtccgcctc
     4381 cgtgaacacc accccgcgcg ccgccgcctg caccgcgaac tcgacgttga gcgccgcgtg
     4441 cgagatgtcc tcgccgggag ccgcgtaggg gccgccccag tagttccaga ccaggccgcc
     4501 ggccgggctc gccgtcatca tgccgcgcat gcgcttggcg agcgcggtcg cccgctcgag
     4561 atacaccgcc tcgcccgtga ggtcgtgcag ggccagcagc gccaggccca tcgcgttgga
     4621 ttgattcagc ggctggtcct tgccggcgaa ctcgaggaag tccgcgctcg cgcgaaacac
     4681 gtagtatccg gcgtcgttcc actcgtcgtc gtgatacgcc gcggtcgcga ccgcggccgc
     4741 gcggtagacc tcggccttgg cggcgaagct ctcgccgtcg tacgcgggcg cgtcgcggtg
     4801 gcgccagccg gcgatgaacg ccgcgagctc ggccatcgga tagacgagca tccccgagtg
     4861 caccgcgtag caatatggct cgcccttgtc ctgatacgag gtgttacgcc agcacgcccc
     4921 cgagaccccg cgatagtcgg cgaccccgcg cgcgtcgtcc cgctgctgca acaatgcatc
     4981 gaggtgccgc gccatgcgat cgacgtacat cgggtgatcg gtgcggcgca ccatcgccat
     5041 cagggaccat agtacgtaag attcacccca cgccagc
//
LOCUS       DASWNJ010000907         8510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_193632, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000907 DASWNJ010000000
VERSION     DASWNJ010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 8510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8510
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193632"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..4558
                     /locus_tag="VGB85_15405"
     CDS             26..4558
                     /locus_tag="VGB85_15405"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0135470.1"
                     /translation="MRLLAKAPDDRYQRAHGLAEDLSICLQRLDGDGHIAAFELGQRD
                     LAEALRIPQQLYGREREVQALMDAFTRVQTGRPELLLLAGGAGVGKSMLVHEVHKAIA
                     RNGGRFVTGKFDLLNRDAPFSALARAFDDLVRQLLTEPSTNLAQWRSMLQMALGANGQ
                     LMVDLVPALERLIGPQAPLQTLRPTEARNRFELVFQNFVRVFCTATTPLVLFLDDLQW
                     ADPATLSLLHLLLADPAGGHLLVVGAYRDTEVEAAHPLRAMIQQLGHQRVATTELTLG
                     PLDRASVRELLAASLMRARADESLDVLTGLVFDKTHGNPFFLTQLLVVLHKSGLLRLD
                     TESGAWTWDAAAIASAPIADNVVEFMSAQLRLLDPRTQWLLSVGACIGHEFDLSTLAA
                     VADVSLGQIVAELWPALRAGLVLPLDSSYRLLIAHDEHSGAELHELADLTVLCRFQHD
                     RVQQAALSLSDEQSRQDVHLRIGRRLEARAGGELRGDALFAVVRHLNAAAPKITDPAQ
                     RLALAQLNLEAARAAKKATATAAATGFLEVAIALLPADSWDTLYDLTFALHRERAECD
                     YLSGRADLAEAQFDDLLLRARSNLVKAELHSLRMVLLTSQGRFGESLAVGRTGLALFG
                     DTLPEGPEAIMATFGATLAAIEAELQQRPVDTLADAPALTDPVQRAVLRLLNEAFGSA
                     FLVDARLGSVVTLKQVLLSLRYGHSELSAFGYASHGYILAGPLGRPFDAQRFGQLALQ
                     VIERFPSAEVVSRVHFMVGFYFGLTQPLRTTITHFTLALQLGLDAGELVFSSQAAVFV
                     AMTQLRLGDDLAVVRESIARAEGVLQRTHDVLATAMLMPQQQIVETLRHRTWWGGALE
                     TDTDEERAWLAGVDASGMVVVRCLHQISKLIVLTLFEEYARARDVIAAVEPILFNAVG
                     APYTADFPYYAALTLAALYPSLPEDERAAGLAAIERHRDAIAVLAAHCPENEQHRLLL
                     VEAELARVGGQELTAMDAYERAIDAAHRSGFYHHEALANELCAKFHIASGRTRVAHVY
                     LSQARFLYDRWGAVAKVVDVESRHPYLVMHSEVGTGSSSTTGRAFVTSSLSNALNQGL
                     DLASVLKASQAFAAEIELQPLLEKIIQIMVENAGARRGVLLLTQEGQLNIVAESSAET
                     GPYTLAQPEPLKDSRMLPSSIVYYVAHASDSVILDDAARTGPFTSDSYVAERSTRSVL
                     CTPLVYQTQLIGVLYLENDLTPSVFPEGRLKAIKLIASQAAIAIENANLYANMERLVH
                     KRTDELSRVNESLTASNAELDAFARTVAHDLKNPLGAVTGYSEYLLENLREIEAEEAE
                     KVVENIRRASQTATSIIDELLLLAGVRKQQVQATRLDMAAIVKQVEQRMAYMLDEHDG
                     EIRRPDTWPAAMGYAPWIEEAWTNYISNGLKYGGVPPRLELGADVLDDGRVRFWVQDN
                     GAGISEADRSRLFAEFTRLDEVRAEGHGLGLSIVRRIIERLDGQVGVESRSEGSRFFF
                     TLPAADAAASAVVPEA"
     gene            4572..6374
                     /locus_tag="VGB85_15410"
     CDS             4572..6374
                     /locus_tag="VGB85_15410"
                     /inference="COORDINATES: protein motif:HMM:NF025273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0135471.1"
                     /translation="MLARFAGCLLLVAGCGEAQFPVASTPSSPVAPPATPDATPTKAA
                     ATVGGRLRAYDGGPLRAAEFAVVRNGFIEPVAKGKLSDEGDFRVEVPPGVHSLVVSAV
                     DHAQVVQQIWVESSVEVEGTLGTYGRVTPGETLQVKSELLATDGTMIAGGPGSAARQA
                     DGTFKLELKDLPEGATKLRYQLAGEHGRTYNGPLADTYESDGGGDYWSVLALEGRDSI
                     ALDLAALPPAGKAASLTWRGEPAEVIAMRAYREAWTPRVTQMQESMPRKDGKFIERGE
                     ADRARMAALAAEALAEADAAGTDDARRLLRLVHLDLFMGDDDDAAARARAQWVLEHVD
                     PVDRRLGLFWNLNHLMIRMAMSADAAFSAQVEAWYVRMQANPEPDLALAASELLIYRA
                     RARGEDARIAELYAVIREPRFAGMYTAKRLAQTFDPDRVLQVGKRFPDFSFPSLTAGG
                     PAVTQAGRAGQWYLVEFWATWCGPCVAEMPELHAAYAKVNAAAPGKGKDGLRRLAAVE
                     RPRVEFVSVSFDGAAGEVEAFRDKQWSMPWTHAFVGMAGQMEVMDRFGFAGVPTAVLI
                     DADSKIVAVGEALRGESLLPTLERALTPRAAAAP"
     gene            6413..>8510
                     /locus_tag="VGB85_15415"
     CDS             6413..>8510
                     /locus_tag="VGB85_15415"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HEY0135472.1"
                     /translation="MFEGLLDAVHRGARAEAWHDGVALARSGAVLVTECGPGGWVLRV
                     GDAQVTWAWTAWRCTCAAAQPCVHVVAGAATLDATAKLLQRAPEFVRAEPRRAHRLRS
                     SPEGLHLRVVELAHDASGQVTERPVAGAADAKDPVLAALPADGGELLLTPGNTATVLA
                     ALGHAEDVTIDGKPVLVDAAPLHAQFVALPDDGGGRRQVAVVPGVTGMFADSSIVLCG
                     DTLRRLALPGASEAKPEPPRPAPPVEDRVVAPAPTAVTPERLELRPQWQADEAGFGVR
                     FQAEAADGQRVDLPAERVVRAWRSGTFAVEGIDGGLAWLPQDWLDRHGPILEQYLAAR
                     DAAGKMPRWAKPAAAALCRALDAPPPPELAGLEVLVDGYTGIPSAPLPPELRAALRDY
                     QRQGVDWLCLLRDAGIGALLADDMGLGKTLQTLCAVDGRTLVVAPTSTLHNWAAEVAK
                     FRPELRVNLYHGQKRSLDAAADLTLTSYAVLRLDREALAKVPWAWDVLVLDEAQAIKE
                     SSTATATAAFEIPARWRVALTGTPIENRLTELWSLAHFLNPGLLGGRKDFVARYARRI
                     ELGHTRVAHELQAKIRPFVLRRRKAEVAPELPPRTDVVLRCTLDAHERAVYDSLRATT
                     HAEVARRLAAGGDMIEALELLLRLRQAACHPALVPGQAATRSSKVDLLTEQLTEITGA
                     GHRALVFSQWTSLLDLV"
BASE COUNT         1207 a         2998 c         3056 g         1249 t
ORIGIN      
        1 cccgccggtc ctgtcggaca tcgtcatgcg cctgctcgcc aaggccccgg acgaccgcta
       61 tcagcgcgcg cacggcctcg ccgaagacct ctccatttgc ttacagcgcc tcgatggaga
      121 cgggcatatc gcggccttcg agctcggcca gcgcgatctc gcggaggcgc tgcggatccc
      181 gcagcagctg tacgggcgcg agcgcgaggt gcaggcgctg atggacgcct tcacgcgggt
      241 gcagacgggc cgccccgagc tgctcttgct cgccggcggc gcgggcgtcg gcaaatcgat
      301 gctcgtacac gaggtccaca aggcgatcgc gcgtaacggc ggtcgattcg tcacggggaa
      361 gttcgacctg ctcaaccgcg acgcgccgtt tagcgcgctg gcgcgggcct tcgacgacct
      421 cgtccgccag ctgttgaccg agccgtcgac caacctggcg cagtggcggt cgatgctaca
      481 aatggcgctc ggcgccaacg gccagctgat ggtcgacctg gtcccggcgc tcgagcgcct
      541 gatcgggccg caggcgccgc tgcagacgct gcggccgacg gaggcccgca accgcttcga
      601 gctggtgttc cagaacttcg tgcgggtgtt ctgcacggcg acgacgccgc tggtgctgtt
      661 cctcgacgac ctgcaatggg cggacccggc gacgctgtca ttgttgcacc tgctgctcgc
      721 cgatccggcc ggcggacatt tgttggtcgt cggcgcctat cgtgacaccg aggtcgaggc
      781 ggcccacccg ctgcgcgcga tgatccagca gctcgggcat cagcgcgtgg cgaccaccga
      841 gctgacgctc ggaccgctcg accgcgcgag cgtgcgcgag ctgctggcgg cgagcctgat
      901 gcgcgcgcgg gccgacgagt cgctcgacgt gctcaccggg ctcgtcttcg acaagaccca
      961 cggcaacccg ttcttcctga cccagctgct ggtggtcctg cacaagagcg ggctgctgcg
     1021 cctcgacacc gagtccgggg cgtggacctg ggacgccgcc gcgatcgcgt cggcgccgat
     1081 cgccgacaac gtcgtcgagt tcatgtcggc gcagctgcgc ctgctcgacc cgcggacgca
     1141 atggttgttg tcggtcggcg cgtgcatcgg tcacgaattc gatctgtcga cattggccgc
     1201 cgtcgccgac gtgagcctcg gacagatcgt cgccgagctg tggccggcgc tgcgcgccgg
     1261 gctggtcctg ccgctcgact ccagctatcg cctgctgatc gcccacgacg agcattcggg
     1321 cgccgagctg cacgagctgg cggacctgac ggtgctgtgc cggttccagc acgaccgcgt
     1381 ccagcaggcg gcgctgtcgc tcagcgacga gcagagccgc caggacgtcc acctgcggat
     1441 cggccggcgc ctggaggccc gcgcgggcgg cgagctgcgg ggcgacgcgc tgttcgccgt
     1501 ggtccgccac ctcaacgccg ccgcgccgaa gatcaccgac cccgcgcagc gactcgcgct
     1561 cgcgcagctc aacctcgagg ccgcccgcgc cgccaagaag gcgaccgcga ccgccgccgc
     1621 gaccggcttc ctcgaggtcg cgatcgcgtt gctgccggcg gactcgtggg acacgctcta
     1681 cgacctgacc ttcgcgctgc accgcgagcg cgccgagtgc gactatctct cgggtcgcgc
     1741 cgacctggcc gaggcccagt tcgacgacct gctgctgcgc gcgcgctcca acctcgtcaa
     1801 ggcggagctg cacagcctgc ggatggtgct gctgacctcg cagggccgct tcggcgagag
     1861 cctcgcggtc gggcgcacgg gcctcgcgct gttcggcgac acgctgcccg agggccccga
     1921 ggccatcatg gcgaccttcg gggcgacgct cgcggcgatc gaggcggagc tgcaacagcg
     1981 tccggtcgac acgctggccg acgcgccggc gctgaccgac cccgtccagc gcgcggtgct
     2041 ccgcctgctc aacgaggcct tcggttcggc cttcctcgtc gacgcccgcc tcggctcggt
     2101 cgtcacgctc aagcaggtgc tgctgtcgct caggtatggg catagcgagc tgtccgcgtt
     2161 cggctacgcg agccacggat atatcctcgc cggcccgctc ggccgtccat tcgacgccca
     2221 gcgcttcgga cagctcgccc tccaggtcat cgagcggttc ccgtccgccg aggtggtcag
     2281 ccgggtgcac ttcatggtcg gcttttactt cggcctgacc cagccgctgc gcaccacgat
     2341 cacgcacttt acgctcgcgc tccagctcgg cctggacgcc ggcgagctcg tgttctcgtc
     2401 gcaggcggcg gtgttcgtcg cgatgacgca gctgcgcctc ggcgacgacc tggccgtggt
     2461 ccgcgagagc atcgcccgcg ccgagggggt gctgcagcgc acccacgacg tgctggcgac
     2521 cgcgatgttg atgccgcagc agcagatcgt cgagaccctg cgccaccgca cctggtgggg
     2581 cggggcgctc gagaccgaca ccgacgagga gcgcgcctgg ctggccgggg tcgacgcgtc
     2641 cgggatggtc gtcgtccgct gcctgcatca gatcagcaag ctgatcgtgc tgacattgtt
     2701 cgaggagtac gcgcgggccc gcgacgtgat cgcggcggtc gagccgatcc tgttcaacgc
     2761 ggtcggtgcc ccgtataccg cggacttccc atattacgcc gcgctgacgc tggcggcgct
     2821 gtatccgtcg ctgcccgagg acgagcgcgc ggccgggctg gcggcgatcg agcgccaccg
     2881 cgacgcgatc gcggtcctgg ccgcgcattg ccccgagaac gagcagcacc ggctgctgct
     2941 cgtcgaggcc gagctcgccc gcgtcggcgg ccaggagctc acggcgatgg acgcctacga
     3001 gcgcgcgatc gatgcggcac atcgcagcgg gttttatcac cacgaggcgc tggccaacga
     3061 gctctgcgcc aagttccaca tcgccagcgg ccgcacgcgc gtcgcccacg tgtatctcag
     3121 tcaggcgcgg ttcttgtatg atcgctgggg cgcggtcgcc aaggtggtcg acgtcgaatc
     3181 ccggcacccg tacctggtga tgcacagcga ggtcggcacc ggcagctcgt cgacgaccgg
     3241 ccgcgccttc gtcaccagct cgctgtcgaa cgcgctcaat cagggcctcg acctggcgag
     3301 cgtgctcaag gcctcgcagg cgttcgccgc cgagatcgag ctgcagccgc tgctcgagaa
     3361 gatcatccag atcatggtcg agaacgccgg ggcccgccgc ggcgtcctgc tgctgacgca
     3421 ggaggggcag ctcaacatcg tcgccgagtc ctcggccgag accgggccgt acacgctggc
     3481 ccagcccgag ccgctcaagg actcgcgcat gctgccgtcc tcgatcgtct attatgtcgc
     3541 ccacgcgagc gacagcgtga tcctcgacga cgccgcgcga accggcccgt tcaccagcga
     3601 cagctacgtg gccgagcgct cgactcgctc ggtgctgtgc acaccgctgg tatatcagac
     3661 ccaattgatc ggtgtgctgt atctcgagaa cgacctgacg ccgtcggtgt tcccggaggg
     3721 ccggctcaag gcgatcaagc tgatcgcctc gcaggcggcg atcgcgatcg agaacgccaa
     3781 tctctacgcg aacatggagc gcctggtcca caagcgcacc gatgagctca gccgcgtcaa
     3841 cgagtccttg accgcgagca acgccgagct cgacgccttc gcccgcacgg tcgcccacga
     3901 cctcaagaat ccgctcgggg cggtcaccgg ctactccgaa tacctgctcg agaacctgcg
     3961 cgagatcgag gccgaggagg cggagaaggt ggtcgagaac attcgccgcg cgtcccagac
     4021 cgcgacgagc atcatcgacg agctgctgct gctggccggc gtccgtaagc agcaggtcca
     4081 ggcgacgcgc ctcgacatgg ccgcgatcgt caagcaggtc gagcagcgca tggcgtacat
     4141 gctcgacgag cacgacggcg agatccgcag gcctgacacc tggccggcgg ccatgggtta
     4201 tgcgccgtgg atcgaggagg cctggaccaa ctatatcagc aatgggctca agtacggcgg
     4261 cgtgcctccg cggctcgagc tcggcgccga cgtgctcgac gacggcaggg tccggttctg
     4321 ggtgcaggac aacggcgccg gcatcagcga ggcggaccgc agccggctgt tcgcggagtt
     4381 cacccgcctc gacgaggtcc gcgcggaggg ccacggcctc gggctgtcga tcgttcgccg
     4441 catcatcgag cgcctcgacg gacaggtcgg ggtcgagagc cgcagcgagg gtagccggtt
     4501 cttcttcacg ctgcccgccg cggacgccgc ggcgagcgcg gtcgtacccg aggcgtgata
     4561 tcctcgggcg tatgctcgcc cggttcgctg gttgcctgct actcgtcgct ggctgcggcg
     4621 aggctcaatt tccggtcgcg tcgactccgt caagcccggt cgcaccgccg gcgacgccgg
     4681 acgcgacacc gacgaaggcg gccgccacgg tcggcggtcg cctgcgcgcg tacgacggcg
     4741 ggccgctgcg agcggccgaa ttcgccgtcg tgcgcaacgg gttcatcgag ccggtcgcga
     4801 aggggaagct gtccgacgag ggggactttc gcgtggaggt gccgcccggc gtccactcgc
     4861 tcgtcgtctc cgccgtcgat cacgcgcagg tcgtgcagca gatatgggtc gagagcagcg
     4921 tcgaggtcga gggcacgctc ggcacgtatg ggcgtgtgac tcccggcgag accttgcagg
     4981 tcaagtcgga gctgctggcc accgatggca cgatgatcgc gggcgggccc ggcagcgccg
     5041 cgcgtcaggc cgacggtaca ttcaaattgg aattgaagga cctgccagag ggcgcgacaa
     5101 agctgcgcta ccagctcgcg ggcgagcacg ggcgcacgta taatgggccg ctcgccgaca
     5161 cctacgagag cgacggcggc ggagattact ggtcggtcct cgcgctcgaa ggccgcgact
     5221 cgatcgcgct cgacctcgcc gcgctgccgc ccgccgggaa ggcagcgagt ctgacgtggc
     5281 gcggtgagcc ggcggaggtg atcgcaatgc gggcatatcg cgaggcctgg acgccgcgcg
     5341 tgacgcagat gcaggagagc atgccgcgca aggacggcaa gttcatcgag aggggggagg
     5401 cggacagggc gaggatggcc gcgctcgcgg ccgaggcgct ggcggaggcc gacgccgccg
     5461 gcacggacga cgcccggagg ctgctgcgcc tcgtgcacct cgatctgttc atgggggacg
     5521 acgacgacgc ggcagcacgc gcgcgcgcac agtgggtcct cgagcacgtc gacccggtcg
     5581 accggcggct cggtctgttc tggaacctca accatttgat gatccggatg gcgatgtccg
     5641 ccgacgcggc attctcggcg caggtggagg cgtggtatgt ccgcatgcag gccaacccgg
     5701 agccggacct ggcgctcgct gcgagcgagc tgctcatata ccgcgcgcgt gcgcgcggcg
     5761 aggacgcgcg gatcgccgag ctgtacgccg tgatccgcga gccgcgcttc gccgggatgt
     5821 acacggcgaa gcggctcgcg cagaccttcg atcccgaccg cgtgctgcag gtgggcaagc
     5881 ggttccccga cttcagcttc ccgtcgctca ccgcgggcgg cccggcggtc acgcaggccg
     5941 ggcgcgcggg ccagtggtac ctcgtcgagt tctgggcgac ctggtgcggc ccctgcgtcg
     6001 ccgagatgcc cgagctgcac gccgcgtatg ccaaagtgaa cgcggccgcg ccgggcaagg
     6061 gcaaggacgg gctgcgccgg ctcgcggcgg tggagcggcc cagggtcgag ttcgtgtcgg
     6121 tctcgttcga cggggctgcc ggcgaggtcg aggcgttccg cgacaagcag tggtcgatgc
     6181 cgtggacgca cgccttcgtc ggcatggcgg gtcagatgga ggtcatggac aggttcggct
     6241 tcgccggtgt gccgaccgcc gtcctcatcg acgccgacag caagatcgtc gcggtcggcg
     6301 aggcgctgcg cggcgagagc ctgttgccga cgctcgagcg ggcgctcacg ccccgcgccg
     6361 cggcggcgcc ctgagccgtg acgtcgggcg cggcgcgtgg tacttccgcg tcgtgttcga
     6421 aggcttgctc gacgcggttc atcgcggcgc cagggcggag gcgtggcacg acggtgtcgc
     6481 gctggcccgc agcggcgccg tcctcgtgac ggagtgtggc cccggcggct gggtgctgcg
     6541 cgtcggcgac gcgcaggtaa cctgggcctg gaccgcgtgg cgctgcacgt gcgcggcggc
     6601 ccagccgtgc gtgcacgtgg tcgccggcgc ggcgacgctc gacgcgaccg cgaagctgct
     6661 gcagcgcgcc cctgaattcg tgcgcgccga gccgcgacgg gcccatcgcc tgcgcagcag
     6721 cccggagggc ctgcacctgc gcgtcgtcga gctcgcgcac gacgcgagcg ggcaggtgac
     6781 ggagcgaccg gtcgcgggcg ccgcggacgc gaaagatcca gtgctcgcgg cgctgccggc
     6841 cgacggcggc gagctgctat tgacccccgg caacaccgcg acggtgctcg cggcgctcgg
     6901 acacgccgag gatgtcacga tcgacggcaa gccggtgctc gtcgacgccg cgccgttgca
     6961 tgcgcaattt gtcgcgttgc cggacgacgg cggcgggcgc cggcaggtcg cggtggtgcc
     7021 gggcgtgacc ggcatgttcg ccgacagctc gatcgtactg tgtggagata ccctgcgccg
     7081 gctcgcgctg ccgggcgcga gcgaggcgaa gcccgagcca ccgaggcccg cgccgccggt
     7141 ggaggaccgc gtggtcgcgc cggctccgac cgcggtcacg ccggagcgcc tcgagctgcg
     7201 accgcagtgg caggccgacg aggccggctt tggcgttcgt tttcaggccg aggcggcgga
     7261 cggccagcgt gtcgacctcc cggccgagcg cgtggtccgg gcctggcgat cgggcacctt
     7321 cgcggtcgag ggcatcgatg gtggcctcgc atggttgccg caggactggc tcgatcgcca
     7381 cgggccgatc ctcgagcaat acctcgcggc gcgcgacgcc gcgggcaaga tgccgcgctg
     7441 ggccaagccc gcggccgcag ccctgtgtcg cgcgctcgat gccccgccgc cgccggagct
     7501 cgccggcctc gaggtattgg tcgacggtta taccggcatc ccctcggccc cgctgccgcc
     7561 cgagctgcgc gccgccctgc gcgattatca gcggcagggc gtcgactggt tgtgcctgct
     7621 gcgcgacgcc ggcatcggtg cattgctcgc cgacgacatg ggcctcggca agacgctcca
     7681 gacattgtgt gcggtcgacg gccgcacgct ggtggtcgcg ccgacgagca ccctgcacaa
     7741 ctgggccgcg gaggtcgcga agtttcgccc cgagctgcgc gtcaacctct accacgggca
     7801 aaagcgcagc ctcgacgccg ccgccgacct gacgctgacg agctacgcgg tgctgcgcct
     7861 cgaccgcgag gcgctggcca aggtgccgtg ggcgtgggac gtgctggtgc tcgacgaggc
     7921 ccaggcgatc aaagagtcgt cgaccgcgac ggcgaccgcg gcgtttgaaa ttccggcgcg
     7981 ctggcgcgtc gcgctgaccg gcacgccgat cgagaatcgc ctgaccgagc tgtggagcct
     8041 cgcgcatttc ctcaatcccg ggctgctcgg cggtcgcaag gacttcgtcg cccgctacgc
     8101 gcggcgcatc gagctcgggc acacgcgcgt cgcccacgag ctccaggcca agatccgccc
     8161 gttcgtgctg cgccggcgca aggccgaggt cgcgccggag ctgccgccgc gcacggacgt
     8221 cgtgctgcgc tgcaccctcg atgcccacga gcgggccgta tacgacagcc tgcgggcgac
     8281 cactcatgcg gaggtcgccc ggcgcctcgc cgccggcggc gacatgatcg aggcgctcga
     8341 gctgctgctg cggctgcgcc aggccgcgtg tcacccggcg ctggtgcccg ggcaggccgc
     8401 gacgcgctcg agcaaggtcg acctgctcac tgaacagctg acggagatca ccggggcagg
     8461 gcaccgcgcg ctggtgttct cgcagtggac ctcgctgctc gacctcgtcg
//
LOCUS       DASWNJ010000908         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_299848, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000908 DASWNJ010000000
VERSION     DASWNJ010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299848"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VGB85_15420"
     CDS             <1..538
                     /locus_tag="VGB85_15420"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY0135473.1"
                     /translation="AAGLGLPDAAIVAGLRSFGAAAGDNPGRADIAEVDGVRIFLDFA
                     HNPDGIRSLAGLLTTLRGPGRLVVSISVAGDRDDEAIRAVARATYELRPDRILVRDLE
                     HYLRGRPPGEVPALLRATFLALGTPPEAIEAVPDEVQALRRGLAWARPGDVIAILPHV
                     DRDEVQTELGAITGRRRA"
     gene            614..1177
                     /locus_tag="VGB85_15425"
     CDS             614..1177
                     /locus_tag="VGB85_15425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0135474.1"
                     /translation="MGSPTQISDPELVVAMARGDNSALGVLYDRHSGILLATAVRILG
                     DQREAEDIVHDVLLEAWQKAGDYDRARGSVRTWLLVRLRSRALDRWRRVSRIRVQTME
                     ARTLDEFLPALADDPGQTLDHARVRRAVEELPEPQRRVLELAYFEGLSSTEVADQLAI
                     PIGTVKSRTAAGLAKLRAAMHDSGGTR"
     gene            1174..>1611
                     /locus_tag="VGB85_15430"
     CDS             1174..>1611
                     /locus_tag="VGB85_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135475.1"
                     /translation="MTASHDDRLVEYVLGTLDREARSELERELGPRLDSELAATREAL
                     SALAVALPPARPPAALRERLMASARGPARFAPFIDRLARLLDVAADRARELLASLEHP
                     GTWLATPGPNVSLVHLLGGPATAGADVGFVRVDAGTSFPPHRHV"
BASE COUNT          206 a          687 c          506 g          212 t
ORIGIN      
        1 ggccgccggc ctcggcttgc cggacgcggc gatcgtcgcc ggcctgcgca gcttcggcgc
       61 cgcggccggt gacaaccccg gccgggccga catcgccgag gtcgacggcg tgcgcatctt
      121 cctcgacttc gcccacaacc ccgacggcat ccgcagcctc gccggcctgc tgacgaccct
      181 gcgcggcccc ggccgtctgg tcgtcagcat cagtgtcgcc ggcgaccgcg acgacgaggc
      241 gatccgcgcg gtcgcccgcg ccacttacga gctgcgtccc gaccgcatcc tcgtccgcga
      301 cctcgagcat tacctgcgcg gccgcccgcc cggcgaggtc ccggcgctgc tgcgggccac
      361 cttcctcgcc ctcggcacgc cccccgaggc gatcgaagcc gtcccggacg aggtccaggc
      421 cctgcgccgc ggtctcgcct gggcccgccc cggcgacgtg atagcgatcc tcccgcacgt
      481 cgaccgcgac gaggttcaaa ccgagctcgg cgcgatcacc ggccgtcgcc gcgcttgacg
      541 gacccctcgg cccccggcga tcgtctgcca cccgtcggca caggacccga cgatccgccc
      601 tatccgacgc accgtgggat ccccgaccca gatcagcgac cccgagctcg tcgtggccat
      661 ggcccggggg gacaacagcg cgctgggcgt gctctacgac cgtcactcgg ggatcctgct
      721 ggcgaccgcc gtccgcatcc tcggtgatca gcgcgaggcc gaggacatcg tccatgacgt
      781 gttgttggag gcctggcaaa aggccggcga ttacgatcgc gcgcgtggga gtgtccgcac
      841 gtggttgctc gtacgcctcc gaagtcgagc actcgatcgc tggcgccgtg tcagtcgtat
      901 cagggtccag accatggagg cacgcaccct cgacgaattc ctgccggccc tcgccgatga
      961 ccccgggcaa accctcgacc acgcccgggt ccgccgcgcc gtcgaggaat tacccgagcc
     1021 gcaacgacgc gtgctcgagc tcgcctactt cgagggcctg tcctccaccg aggtcgccga
     1081 ccagctcgcc atcccgatcg gcaccgtgaa gtcgcgtacc gccgcagggc ttgccaagtt
     1141 gcgcgccgcg atgcacgact ccggggggac gcgatgaccg ccagccacga cgatcgcctg
     1201 gtcgagtacg tgctcggcac cctcgaccgc gaggcccgca gcgagctcga gcgagagctc
     1261 ggcccgcgcc tcgacagcga gctcgccgcg acccgcgagg ccctctccgc cctcgccgtc
     1321 gccctcccgc ccgcgcgccc acccgcggcc ctgcgcgagc gcctgatggc ctcggcccgc
     1381 ggccccgccc gcttcgcccc cttcatcgac cgcctcgccc gcctcctcga cgtcgcggcc
     1441 gaccgcgccc gcgagctgct cgccagcctc gagcacccgg gcacctggct cgccacgccc
     1501 ggccccaacg tctcgctcgt ccacctcctc ggcggccccg cgaccgccgg cgccgacgtc
     1561 ggcttcgtcc gcgtcgacgc cggcaccagc ttcccgcccc accgccacgt c
//
LOCUS       DASWNJ010000909         5926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_368982, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000909 DASWNJ010000000
VERSION     DASWNJ010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368982"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3458
                     /locus_tag="VGB85_15435"
     CDS             <1..3458
                     /locus_tag="VGB85_15435"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0135476.1"
                     /translation="SLRRGEPERRSLLLALGALYVHGAQPAWRRVAAQGRRVELPTYP
                     FQRQRHWNTSAISSVPTLGHGAEVTTPEDQGASLFHLRWISVPRPDGERGLAGTWLLF
                     AGDGVADIAATMVAAGLRVITVAPGPTFTRPDPDHVTLDPTRPEHIDQLFTALAVEGV
                     RPGGCVYMGGSGPADALDETVTAGALLLARALLAQPWVDTCPLWFVTRGVHGGDASDE
                     AVIRQAPLWGLGRVLQSEAPALGCRLLDLDAADLTGPGAALMRELRLEDGENQVRWRG
                     GVREVARLLPHAVGPAAPVAAGAAHFDPDGTYLITGGLGGLGRALAAWLLGHQGVKHL
                     VLTGRREPDAEAVAWLTTQRAGGARVEVIAADIGDPAQVDALLATMARDLPPLVGVFH
                     LAAVLDDATIANQNVARLQAVLAPKLRGAWHLHRATASLRLQHFVLFSSAAGVFGTPG
                     QANYAAANAGLDALAELRRQRGLAGLSLAWGPWAELGKAAEQSSTTQQRWRAMGLEGI
                     SVEQGMRAMTLALAGNEACATVLAVRRTGLEIQGLVPPLLRRIARNTGHATGRLLDRL
                     RASDPGRRLGVLEMALQTLVIQRLGLRDANQLGPETPLFDLGMDSLMATELRQALSDG
                     LAHPLPPTLIYEHKTIQAIAAWIFEHVLPVVQPRGPAPAPEVAAPAPTPRAGNPWLFV
                     PVPRPGAELRLICLPYAGGFAQDFAPWARLVPEHIEVCAVQSPARRERQTEPWPRTFD
                     ELVDELVVALRPHLDRPFAILGHCLGAIVGYEVVRRLTEQGGPQPLHFFAAGALPPHQ
                     YMLNDVAARSDESLLALLQLLDTPRLGTLASEPALLRAALEFIRGDFRLATSYRHRKG
                     APLDLPITVFAGLDDMICPHTAMDPWSRHGRDVRVVRLPGSHYFIESRRPVVIDEVLA
                     VLSQPRSPAPTLPGDDDASITLIAVPGIGGAGSDFAPLVGLVGPGITVISDDPGVGID
                     NVAVRAEALVAQVRARATRRYALLGHDLGALVAYEAIRRLRAVGDELPAHLFVLAAMA
                     PHVSYVPPIGDRSGPMLVDALTTLGVPVRADAAPAQLRADFRALASYVWTDETALDTP
                     ISACLGVRDTIIPEAALTAWDDLTTRGLTVHRLDAGHDLPAAAPVAIASIVARALSGG
                     RVIAR"
     gene            complement(3432..5450)
                     /locus_tag="VGB85_15440"
     CDS             complement(3432..5450)
                     /locus_tag="VGB85_15440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0135477.1"
                     /translation="MLKTVKVPPPLEPLFESAQEYVGRFFAARSEVPEEAKIEIFGQR
                     YVLVRARAMSVEFFEIVQRLYADKGQDQALGVARSLVFDIAHAMGLADANEFAEQMNL
                     VDPLARLSAGPVHFSHSGWAYVDIFEESHPSPDENFYLLYDHPYSFESDSWIDAKKRT
                     EVPICVMNSGYSSGWCEASFGVPLVSSEILCRARGDEHCRFIMAHPSRIQEYIQAYIE
                     SQPKLAANPPRYEIPGFFSRKRQEDELRNREEQYRGIFEAGTNGLLILGEDGTVVQAN
                     PAALRLFRCEAAALFGRSITTVWPELEFVDQFRAQIEATGHYQAEGVTLTLNDQRYFV
                     EVRGIRFRFQDRDHLLAIVTDITDKKQIQVALRRAHDELEGHVRDRTAALDRANHQLT
                     LLNEKLTVARDTAVDANVAKSAFLASMSHEIRTPLNAIIGCSELLEEEFLEGNGRPTD
                     LSDLTKIRTAARLLLDLIDDILNVSKIEAGKMELFIEDFPLDELVQEIVAAVTPTAAR
                     NGNTLDFQIEPNIGTVRLDRVKVKQILLNLMSNACTFTNDGLVHLRAVRERGPDREDL
                     VLHIRDTGSGIDQEQLDRLFHPFRQADESPPRASGGSGLGLSISLHFAEMMAGRIDAT
                     SVPGQGSTFTVRLPAALPRGTPVALPRDDAAALPRDNPAALPRDDAAS"
     gene            complement(5532..>5926)
                     /locus_tag="VGB85_15445"
     CDS             complement(5532..>5926)
                     /locus_tag="VGB85_15445"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0135478.1"
                     /translation="ALLSRAGGVIINISATLHYAGTPLQTHAASAKAGIDALTRSLAM
                     EWGAAGIRINALAPGPIDGTEGITRLLPPELRARAERIIPLRRFGSTRDVADAALFLA
                     SPASAYITGAVLVVDGGQWLAGSMALTE"
BASE COUNT          791 a         2168 c         2062 g          905 t
ORIGIN      
        1 ccagcctgcg gcgcggcgag cccgagcggc ggtcgctgct gctcgccctc ggcgccctct
       61 acgtgcatgg cgcgcagccg gcgtggcgcc gcgtcgccgc gcaggggcgg cgcgtcgagc
      121 tgccgaccta tccgttccag cgccagcgtc actggaacac ctccgcgatc agctccgtgc
      181 cgacgctcgg ccacggagcc gaggtcacca cgccggagga ccagggcgcc tctctgtttc
      241 acctgcgctg gatctccgtg ccgaggccgg acggcgagcg cggcctcgcg ggcacctggc
      301 tgctgttcgc cggcgatggc gtcgccgaca tcgccgcgac catggtcgcc gctggcctgc
      361 gggtgatcac tgtcgcgccg gggccgacgt tcacccggcc ggaccccgac cacgtcaccc
      421 tcgacccgac ccggcccgag cacatcgatc agctgttcac tgcgctggcg gtcgagggcg
      481 ttcgtcccgg cggatgcgtg tacatggggg gctccgggcc cgcggacgcg ctggacgaga
      541 cggtcaccgc gggcgcgctc ctgctcgccc gggccctgct cgcgcagccc tgggtcgaca
      601 cctgcccgct gtggttcgtc acgcgcggcg tccacggcgg tgatgcgtcc gacgaggccg
      661 tcatccggca ggcgccgctg tggggcctcg gtcgggtgtt gcagtccgag gccccggcgc
      721 tcgggtgtcg cttgctcgac ctcgacgccg ccgacctgac cgggccgggc gccgcgctga
      781 tgcgcgagct ccggctcgag gacggtgaga accaggtccg ctggcgcggc ggggtccgcg
      841 aggtcgcgcg cctgctgccg cacgcggtcg gccccgcggc gcccgtcgca gccggcgcgg
      901 cgcacttcga ccccgacggg acctatctga tcacgggtgg tctgggcggc ctgggtcgcg
      961 ccctcgcggc ctggctgctc ggtcaccagg gcgtcaagca cctcgtgctc accggccgcc
     1021 gcgaacctga cgccgaggct gtcgcctggc tcaccacgca gcgggccggc ggtgcgcggg
     1081 tcgaggtgat cgcggccgac atcggcgacc ccgcgcaggt cgacgccctc ctggcgacca
     1141 tggcgcgcga cctgccgccc ctggtcggcg tgttccacct cgccgccgtc ctcgacgacg
     1201 ccacgatcgc caatcaaaat gtcgcgcgcc tgcaggccgt gctcgcgccc aagctgcggg
     1261 gcgcctggca cctgcatcgg gcgaccgcct cgttgcgctt gcaacatttc gtgctgttct
     1321 cctcggcagc cggcgtcttc ggcaccccgg gccaggccaa ttacgccgcc gccaacgccg
     1381 ggctcgacgc gctcgccgag ctgcggcgac agcggggcct cgcggggctc agcctcgcgt
     1441 ggggcccatg ggccgagctc ggcaaggccg ccgaacagag ctcgacgacc cagcagcgct
     1501 ggcgcgcgat gggcctcgag ggcatctccg tcgagcaggg gatgcgggcc atgacgctgg
     1561 cgctggcggg gaacgaggcc tgcgccacgg tcctcgcggt gcggcgcact ggcctcgaga
     1621 tccagggcct ggtgccgccg ctgctccggc ggatcgcccg caacaccgga cacgccaccg
     1681 gccgcctgct cgatcgcctg cgcgcgagcg acccgggccg ccggctcggg gtcctcgaga
     1741 tggcgctcca gaccctcgtc atccagcggc tcggcctgcg cgacgccaac cagctcggcc
     1801 ccgagacccc gctgttcgac ctcggcatgg actcgctgat ggcgaccgag ctgcggcaag
     1861 ccctctccga cggcctcgcg cacccgctcc cgccgacgct gatctacgag cacaagacga
     1921 tccaggcgat cgccgcctgg atcttcgagc acgtgctccc ggtcgtgcaa ccccgcggcc
     1981 cggcgccggc ccccgaggtc gccgcgccag cgccgacccc acgcgccggc aacccgtggc
     2041 tgttcgtccc ggtcccgcgc ccgggcgccg agctgcgcct gatctgcctg ccctacgccg
     2101 gtggcttcgc gcaggacttc gcgccgtggg cccgtctcgt gccggagcac atcgaggtct
     2161 gtgccgtgca atcccccgcg cggcgcgagc ggcagaccga accgtggccg cggaccttcg
     2221 acgagctcgt cgacgagctc gtcgtcgcgc tgcgccccca cctcgaccgc ccgttcgcca
     2281 tcctcggcca ctgcctcggc gccatcgtcg gctacgaggt cgtgcgccgc ctgaccgagc
     2341 aaggcggtcc gcagccgctc catttcttcg ccgcgggcgc cctcccgccg catcagtaca
     2401 tgctcaacga cgtcgcggcg cgctcggacg agtcgttgct cgcgctgctc cagctgctcg
     2461 acactccgcg gctcggcacc ctcgcgtccg agccggcgct gctgcgcgcg gcgctcgagt
     2521 tcatccgcgg cgacttccgt ctggccacat cctatcggca tcgcaagggc gccccgctcg
     2581 acctgccgat cacggtgttc gccggcctcg acgacatgat ctgcccgcac accgcgatgg
     2641 acccgtggag ccgccacggg cgcgacgttc gggtcgtgcg tctgcccggg agccactact
     2701 tcatcgagag ccggcgcccg gtcgtcatcg acgaggtcct cgcggtgctc agccagccga
     2761 ggagcccggc gcccacgctg cccggggacg atgacgcctc gatcaccctg atcgccgtgc
     2821 ccggtatcgg cggcgccggc tcggacttcg cgccgctcgt cgggctcgtg ggacccggga
     2881 tcacggtgat ctccgacgat cccggcgtcg gcatcgacaa cgtcgcggtg cgggccgagg
     2941 cgctggtcgc ccaggtgcgc gcgcgcgcga cccggcgcta tgcgctgctg ggacacgacc
     3001 tcggggcgct cgtcgcctac gaggcgatcc gtcggctccg cgccgtcggc gacgagctcc
     3061 cggcccacct gttcgtgctc gccgccatgg ccccgcacgt ctcctacgtc ccaccgatcg
     3121 gcgaccgcag cgggccgatg ctggtcgacg cgctcaccac cctcggcgtg ccggtccggg
     3181 ccgacgccgc gcccgcgcag ctgcgcgcgg actttcgggc gctcgccagc tacgtgtgga
     3241 ccgacgagac cgcgctggac accccgatca gcgcgtgcct cggggtgcgt gacacgatca
     3301 tcccggaggc cgcgctcacg gcgtgggacg acctcacgac ccgcgggctc actgtccacc
     3361 ggctcgatgc ggggcacgac ttgccagcgg cggcgcccgt cgccatcgcg tcgatcgtcg
     3421 cgcgtgcgct gtcaggaggc cgcgtcatcg cgcggtagcg cggccgggtt gtcgcgcggc
     3481 agcgcggccg cgtcatcgcg cggtagcgcg accggggtgc cgcgcggtag cgcggccggg
     3541 aggcgcacgg tgaacgtcga gccctggccg ggcacgctgg tcgcgtcgat ccggccggcc
     3601 atcatttcgg cgaagtggag gctgatcgac agcccgaggc cggacccgcc ggacgcgcga
     3661 gggggcgact cgtcggcctg gcgaaacggg tgaaagagcc ggtcgagctg ctcctggtcg
     3721 atgcctgacc cggtgtcacg gatatgcagc accaggtcct cgcggtccgg gccgcgctcg
     3781 cggaccgccc gcaggtgaac gaggccgtcg ttggtgaacg tgcacgcgtt cgacatcagg
     3841 tttagcaaga tctgcttgac cttgacccgg tcgagtcgga ccgtgccgat gttcggctcg
     3901 atctggaagt cgagggtatt cccgttgcgc gcggcggtcg gtgtgacggc ggcgacgatc
     3961 tcctggacga gctcgtcgag cgggaagtcc tcgatgaaca gctccatctt gccggcttcg
     4021 atcttcgaga cgttgaggat gtcgtcgatc aggtcgagca ggaggcgggc ggccgtgcgg
     4081 atcttcgtga ggtcggagag gtcggtcggg cggccgttgc cctccaggaa ctcctcctcg
     4141 agcagctcgc tgcagccgat gatcgcgttt agcggcgtgc ggatctcgtg gctcatgctg
     4201 gccaggaagg cgctcttcgc cacgttggcg tcgaccgccg tgtcacgcgc gacggtcaac
     4261 ttctcgttta gcagagtcag ctggtgattg gcccggtcga gcgcggcggt gcgatcccgg
     4321 acatgcccct cgagctcgtc atgggcccgg cgcagggcca cctggatctg tttcttgtcg
     4381 gtgatatcgg tgacgatcgc cagcaagtga tcgcggtcct gaaagcggaa gcggataccg
     4441 cggacctcga cgaagtaccg ttggtcgttg agcgtcaagg tcacgccctc ggcctgataa
     4501 tggccggtcg cctcgatctg ggcgcgaaac tggtccacga actccagctc gggccatact
     4561 gtcgtgatcg accttccgaa cagcgccgcc gcctcgcagc gaaacagccg caacgcggcc
     4621 gggttggcct ggacgaccgt accgtcctcg ccgaggatga gcagcccgtt ggtgccggcc
     4681 tcgaagatac cgcggtactg ctcctcacgg ttgcgaagct cgtcctcctg gcgcttgcgc
     4741 gagaagaacc ccgggatctc gtaccgcggc gggttggcgg ccagcttcgg ctgactctcg
     4801 atataagcct ggatgtactc ctggatgcgc gacgggtgcg ccatgatgaa tcggcagtgc
     4861 tcgtcgccgc gggcccggca caagatctcc gaggagacca gcggcacgcc gaagctcgcc
     4921 tcgcaccagc ccgaggagta gcccgagttc atgacgcaga tcgggacctc ggtccgcttc
     4981 tttgcgtcga tccaggagtc cgactcgaat gaatacgggt ggtcgtaaag gaggtagaag
     5041 ttttcgtcgg gggaggggtg gctctcctcg aagatatcga cgtacgccca gccggaatgt
     5101 gagaagtgca ccggcccggc cgacagccgc gccagcggat ccacgagatt catctgttcg
     5161 gcgaattcat tggcgtcggc cagccccatc gcgtgggcaa tgtcgaatac caggctgcgc
     5221 gcgaccccca gcgcctggtc ctgacccttg tcggcgtaca gccgctggac gatctcgaag
     5281 aattcgaccg acatcgcccg cgcccggacg agcacatagc gctgcccgaa tatctcgatc
     5341 ttcgcctcct cggggacctc gctccgcgcc gcgaagaatc gaccgacata ctcctgcgcc
     5401 gactcaaaca gcggctccaa cggcggcggc accttgaccg tcttgagcat ccgacgcgag
     5461 cgtacacggg tgtgtttcgc ggggcaagcg aggtccgtcg ggttgccgcg ttcgaagcgt
     5521 atgaattata ttcactcggt cagcgccatc gagcccgcga gccactgtcc gccgtcgacg
     5581 acgagcaccg caccggtgat gtacgcgctc gcgggcgagg cgaggaacaa cgcggcgtcg
     5641 gcgacgtcgc gtgtgctgcc gaaccgacgc agcgggatga tccgctcggc gcgcgcgcgc
     5701 agctcgggcg gcagcagccg cgtgatgccc tcggtcccgt cgatcggccc gggtgcgagg
     5761 gcgttgatgc ggatcccggc ggccccccac tccatcgcca gcgagcgcgt cagcgcgtcg
     5821 atgccggcct tggcgctcgc ggcgtgcgtc tgcagcggcg tgccggcgta atgcagggtc
     5881 gcggagatgt tgatgatcac gccgcccgcc cgtgagagca gcgcga
//
LOCUS       DASWNJ010000910         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_299875, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000910 DASWNJ010000000
VERSION     DASWNJ010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299875"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2020)
                     /locus_tag="VGB85_15450"
     CDS             complement(<1..>2020)
                     /locus_tag="VGB85_15450"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY0135479.1"
                     /translation="LVDFIVTLWTWLVAWTIRGTGSGSGRATGERRERDRLLGLLYDE
                     AGRILYFEMRDLVFVQAVLRQLRPAARLGVSPEQARADSLYAILLGTIDRRRGAARAA
                     ARALRTAQAVGDPATLARVQLYAAWTHEFCGRVAEAELRLREVLRDDNRWLESWWYIT
                     GTTTLCVSLWVRGHAREALDLANRAISQIEERSSSRGREREQQLAVLHAMVPAIMAML
                     GKGDRGAVFLDRARAFFTARPNDRFWHMIMVALSLAYHVEEGELGEPIELAINHVRGL
                     GMHPDRVSFYARYFYAFQVHARQAQALRAGESERVVAINRLEAAVAELSQVPEHPVWR
                     SFLLPARAVLAWLSQRPGEALSLLTEAETLSLATDHLWLRFEVLRLRARVFVAQGRPA
                     AGEREARFAVELAQQHGWDSRVALVRGELGIAGVGSRAGSAPGGLVDDMQTLKLRRHL
                     DALLELSVASASVRTPAEQARVALDELVRILAAEHAYLFLSRGDEGPLELKAGRDLHG
                     HDLGELAGYSRTIVEQVRQTRAPVVLSSGTQGPFSGAESVRVHDLRSVVAAPLLMKDR
                     LVGVVYLDNRLVRGAFSPDDVEILVSIANHIAIGLETARAAALEVQVAASVRERAILL
                     EHATRSVGIGIALLRDDGSLGEESPTLRQMTDPWPSLAAWWAQARVVAP"
BASE COUNT          292 a          691 c          799 g          238 t
ORIGIN      
        1 cggggcgacg acccgcgcct gggcccacca ggcggcgagg ctcggccacg ggtcggtcat
       61 ctggcgcagg gtcgggctct cctcgccgag gctgccgtcg tcgcgcagca gggcgatgcc
      121 gatgccgacg ctgcgggtcg cgtgctcgag caggatcgcg cgctcgcgga cggaggcggc
      181 gacttgcacc tcgagcgcgg cggcgcgggc ggtctcgagg ccgatcgcga tgtgattggc
      241 gatcgacacc aggatctcga cgtcgtcggg gctgaaggcg ccgcggacga ggcggttgtc
      301 gaggtagacc acgccgacga ggcggtcctt catcagcaag ggggccgcga cgacgctgcg
      361 caggtcgtgg acccgcacgc tctcggcgcc ggagaacggg ccctgggtgc cgctgctgag
      421 caccaccggc gcccgggtct ggcgcacctg ctcgacgatc gtgcggctgt aaccggcgag
      481 ctcgccgagg tcgtgaccgt gcaggtcgcg gccggccttg agctcgagcg ggccctcgtc
      541 tccgcgcgac aggaacaggt aggcgtgctc ggccgcgagg atccggacca gctcgtcgag
      601 cgcgacgcgg gcctgctccg ccggcgtccg caccgaggcc gacgcgacgc tcagctcgag
      661 caaggcgtcg aggtgacggc gcagcttgag cgtctgcatg tcgtcgacga ggccgccggg
      721 ggccgagccg gcccgcgacc ccacgccagc gatgccgagc tcgccgcgca ccagcgcgac
      781 gcgcgagtcc cagccgtgct gctgcgcgag ctcgacggcg aagcgggcct cgcgttcacc
      841 ggcggccggc cggccctggg cgacgaagac gcgggcgcgc aggcgcagga cctcgaagcg
      901 cagccacagg tggtcggtcg cgagcgagag cgtctcggcc tcagtcagca gggagagggc
      961 ctcgcctggc ctttgggaca gccaggcgag gacggcccgc gcgggcagga ggaagctgcg
     1021 ccagaccggg tgctcgggga cctgcgacag ctcggcgacc gcggcctcga ggcggttgat
     1081 cgccacgacg cgctcggact cgccggcgcg cagggcctgg gcctggcggg catggacctg
     1141 gaacgcgtag aagtagcggg cgtagaacga gacgcggtcg gggtgcatgc cgaggccgcg
     1201 gacgtggttg atcgccagct cgatcggctc gccgagctcg ccctcctcga cgtggtaggc
     1261 gagcgagagg gcgaccatga tcatgtgcca aaaacggtcg ttggggcgcg cggtgaagaa
     1321 ggcgcgcgcg cggtcgagga acaccgcgcc gcggtcgccc ttgccgagca tcgccatgat
     1381 cgcggggacc atcgcgtgca gcacggcgag ctgctgctcg cgctcgcgac cgcgggacga
     1441 gctgcgctcc tcgatctgcg agatcgcacg gttggcgaga tcgagggcct cgcgcgcgtg
     1501 gccgcggacc cacaagctga cgcacagggt cgtggtgccg gtgatgtacc accacgactc
     1561 gagccagcga ttgtcgtcac gcaggacctc gcgcaggcgc agctcggcct cggcgacgcg
     1621 gccgcagaac tcgtgggtcc aggcggcgta gagctggacg cgggcgaggg tcgccgggtc
     1681 gccgaccgcc tgggcggtgc gcagagccct ggcagcggcc cgcgcggccc cgcggcggcg
     1741 gtcgatcgtg ccgagcagga tcgcgtacag cgagtcggcg cgggcctgct cgggcgagac
     1801 gccgaggcgc gcggcgggcc gaagctggcg cagcacggcc tgcacgaaca cgaggtcccg
     1861 catctcgaag tagaggatcc gcccggcctc gtcgtagagc aggccgagca ggcggtcgcg
     1921 ctcgcgtcgc tcgccggtcg cacgcccaga gccgctgccg gtgccgcgga tcgtccacgc
     1981 gaccagccag gtccacaggg tgacgatgaa gtcgacgagg
//
LOCUS       DASWNJ010000911         2200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_328279, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000911 DASWNJ010000000
VERSION     DASWNJ010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328279"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..2041)
                     /locus_tag="VGB85_15455"
     CDS             complement(26..2041)
                     /locus_tag="VGB85_15455"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0135480.1"
                     /translation="MLARIFTSADELRRWLCMRLPDIYADLPGGNASLSDLAYAAQGL
                     LERTGMIDDELLDALADFSPAHGPAVVELSKWLGRVGPPAPPPRTHAPTASVLATLHP
                     LHAAEPSDRREIKAPEAKSHAPRTQSGLPPHERGQVLGNGRYVLRSLLGRGGFATVWR
                     ARDTREGRDVAVKLMHPQDAQDVARRERFFRGARAMQHLQHPAIVRVLDPRAGESPQP
                     FFVMEIVEGTDLHEALAGKPHIDLHEILDIACTIGEALAEAHLRGMVHRDIKPANILI
                     DRRGAPHLTDFDLVRARGISGGTRTGGMGTVLYAAPELVQNAEDADARADIYGLGMTV
                     LFMLFGNDPPYKDIVRSPEAILASVACPPTIKRVVLRAIDWDREQRFPDVGSFCAALR
                     DAGPHPRQHVPPRPTPQPQPSPALQLQPVAKSQRAPTRKHTSHELWGLCSLAVATVIV
                     VLALGPTPPGETVPGGPQAASGEPSPGAQPPLPSALPAGASPLRARSGSIADAEPRQT
                     TGLPAEVASARPEPKSSIVTPSAPRGITRPPKISGPHRAASSPPPAEVTETAAPIEPP
                     TAPPPKPSLEELIGALEDEIKRYGDSKGADCLSEEIGLEHDKAWAVDAVVSMAVEVGA
                     DGRIDGSRILGSGEQGDLALCMVKKLNGRQLTTAPGSAVTVNVDFGF"
BASE COUNT          350 a          694 c          777 g          379 t
ORIGIN      
        1 ttgaatggtg cgaaatgcca ggctttcaga atccgaagtc gacgttcact gtcacggcgg
       61 atccaggcgc ggtcgttaat tgtcgcccgt tcaacttctt gaccatacac agcgccagat
      121 cgccttgctc gcctgatccg agaattcggg atccgtcgat ccggccgtcc gcgccgactt
      181 ccacggccat gctgacgacc gcgtcgaccg cccaagcttt atcgtgttcc agtccgattt
      241 cctcggacag gcagtccgcg cccttgctat cgccgtatcg cttgatctcg tcttccagcg
      301 ccccgatcag ctcctccagg ctcggcttcg gcggaggcgc cgtcggcggc tcgatcggag
      361 cggcggtctc cgtcacctcg gccgggggcg gcgaagaagc ggctcgatgg ggcccggaga
      421 tcttgggagg tcgggtgata ccccgaggag cggacggcgt cacgatcgac gacttcggtt
      481 cgggccgggc ggaagctacc tccgcaggca gtcccgtggt ctggcgtggc tctgcatcgg
      541 cgatgctgcc cgaccgggct cggagtggag aagcgccggc gggaagggcc gatggaagtg
      601 gtggttgcgc accgggagag ggctcgcccg aagctgcctg cggcccaccc ggaacagtct
      661 cgcccggagg cgttggtccc agcgcgagca cgacgatgac ggtggccacc gcaaggctgc
      721 agaggcccca gagctcatgg gaagtatgct tccttgttgg ggcccgctgt gacttcgcaa
      781 ccggctgcaa ctgcagggcc gggctcggct gaggctgcgg agtcggccgg ggcggaacgt
      841 gctgtctcgg atgtgggccg gcgtcgcgta gcgcggcgca aaaactcccg acatcaggaa
      901 agcgctgctc ccggtcccag tcgatagcgc gcaggacgac ccgtttgatc gtgggagggc
      961 aagcgacgga cgcgaggatg gcctcggggc tgcggacaat gtccttgtag ggtgggtcgt
     1021 tgccgaagag catgaacagc accgtcattc ccagaccgta aatatcggca cgggcgtcgg
     1081 catcctctgc gttttgaacc agctccgggg ctgcatacag gacggtaccc atgccgccgg
     1141 tgcgtgtgcc tcccgagatg ccgcgtgctc gcacgagatc gaagtcggta aggtgtgggg
     1201 ccccgcggcg atcgatcagg atgttcgcag gcttgatgtc gcgatggacc atgcctcgca
     1261 aatgcgcctc ggcgagggcc tcaccgatcg tgcaggcgat atcaaggatc tcgtgcagat
     1321 caatgtgtgg cttacccgcg agcgcctcgt gcagatctgt cccctcgacg atctccatca
     1381 cgaagaacgg ctgcgggctc tcgccggcac gcggatccag gacgcggacg atcgcgggat
     1441 gttggaggtg ctgcatcgcg cgcgcgccgc gaaagaaccg ttcgcggcga gcgacatcct
     1501 gcgcatcctg gggatgcatc agcttcaccg cgacgtcgcg gccctcgcgg gtgtcacgcg
     1561 cacgccagac cgttgcgaac ccaccgcgac caagcaggct tcggagcacg tagcggccat
     1621 tcccgagcac ttggccgcgc tcatgggggg gtaggccgct ctgtgtgcgg ggggcgtggc
     1681 tcttcgcctc gggcgctttg atttcccgac ggtcgctcgg ctcggccgcg tgcagcggat
     1741 gtagcgttgc gaggacggag gctgtcggcg cgtgtgtccg aggcggtggc gcgggtggcc
     1801 cgactcgtcc gagccatttg ctgagctcga cgactgcagg gccgtgcgcc ggagagaagt
     1861 cggcgagcgc gtcgagcagc tcgtcgtcga tcatacctgt gcgctcgagc aaaccctgcg
     1921 ccgcgtatgc gaggtcggac agggaggcgt tgccgcctgg caggtccgca tagatatcgg
     1981 gcaggcgcat gcacaaccac ctgcgcagct cgtcggccga agtgaagatg cgcgcgagca
     2041 cggtagcgag cgtatctctg cggcccctgt cgccggggga gggacgcttg aacgatgatg
     2101 ctgtggatgc tgtgccgggc cagtacaacc gaccgcggcc cgtcccggga aggtctggcg
     2161 cctctccgag ccagcgcttc tggctcggcg accaggtcca
//
LOCUS       DASWNJ010000912         2034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_86166, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000912 DASWNJ010000000
VERSION     DASWNJ010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86166"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2034)
                     /locus_tag="VGB85_15460"
     CDS             complement(<1..>2034)
                     /locus_tag="VGB85_15460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0135481.1"
                     /translation="GSGGAEDTVLIRSSTTYLVVEDRGRKINDVRLGPKTLRVGRRPD
                     CDVVVVAPGVAPYHAKIEPFGTGHRIVDLENTTGLVFRGNRVRSHVFNDGDVIRIGDR
                     VTGNFVSLVYQDMGKRAQAPVPAPVHRVPLDRPRVRIGREGCDLTLPSLQVSRVHAEI
                     RRAGTGHEVVDLDSTNGTFIAGARVQTQKLKVGDVVQIGPFKLTYTGKSLDQLEQVGA
                     MRIEARGLTRVTPEGKVILDQVSLVIEPREFVAIVGASGAGKSSLMGALAGYRRADRG
                     VLRVNGDDFYANYDLYRAVLGYVPQDDILHATLTVEDALTYTAKLRLPADTTPAEITA
                     RISRVLEDVEMSANRKQRISDLSGGERKRVSIASELLADPSLFFLDEPTSGLDPGLEK
                     RMMYTLRYLADSGRTVVLVTHATANITQCDLVAFLADGRLVYYGPPAGALQMFGATGG
                     DFADIYTRLHGPASADQIEYGGELAAQYAVWRQHHPKPAKPPSMAELWEMRFRDSEYY
                     RTYVYERSKPAGRRADTEPIAPAGVRLATIAPGHYRFTPDAAPPAVEVKPPVPPRTSQ
                     ARQFGILTRRYVRLLAVDRKNLLILLMQAPIIGVVILLAARANALQSPTSSNGRLVLF
                     LLAVIGVWFGVLNSVREVTKEAKIYRRERLANLRIAPYVGSKVAVLAGLCMIQ"
BASE COUNT          285 a          694 c          737 g          318 t
ORIGIN      
        1 ctctggatca tgcacagccc tgcgagcacc gcgaccttcg agccgacgta gggtgcgatg
       61 cgcaggttgg cgaggcgctc gcggcgatag atcttcgcct ccttcgtcac ctcgcggacc
      121 gagttgagca ccccgaacca caccccgatc accgcgagca ggaacagcac caggcggccg
      181 ttgctcgagg tcggagattg cagggcgttg gcccgcgccg ccagcaggat caccacgccg
      241 atgatcggtg cctgcatcag tagtatgagc aggttcttgc ggtcgaccgc gagcaatcgg
      301 acgtagcggc gcgtcaggat gccgaactgg cgcgcctgcg acgtgcgtgg cggcaccggt
      361 ggtttgacct cgaccgccgg cggtgcggcg tcgggggtga agcggtagtg gcccggggcg
      421 atcgtcgcca ggcggacccc cgcgggggcg atcggctcgg tgtcggcgcg gcgtcccgcc
      481 ggtttgctgc gctcgtacac gtacgtccgg tagtactcgg agtcgcgaaa ccgcatctcc
      541 cacagctccg ccatcgacgg cggcttggcc ggcttggggt ggtgctggcg ccacaccgcg
      601 tactgggccg ccagctcgcc gccgtactcg atctggtccg cgctcgcggg gccgtgcagg
      661 cgcgtgtaga tgtcggcgaa gtcgcctccg gtcgcgccga acatctgcaa ggcgcccgcg
      721 ggcggcccgt agtagaccag gcggccgtcc gcgaggaacg cgacgaggtc acactgcgtg
      781 atgttggcgg tcgcgtgggt gacgagcacc accgtgcggc ccgagtccgc gaggtagcgc
      841 agcgtgtaca tcatgcgctt ctcgaggccg gggtcgaggc ccgaggtcgg ctcgtcgagg
      901 aagaacaggc tcgggtcggc gagcagctcc gacgcgatcg acacccgctt gcgttcgccg
      961 cccgagagat ccgagatccg ctgcttgcgg ttcgcgctca tctcgacgtc ctcgagcacg
     1021 cgcgagatcc gggccgtgat ctcggcgggg gtcgtgtcgg ccggcaggcg cagcttcgcg
     1081 gtgtaggtca gcgcgtcctc gacggtcagc gtcgcgtgga ggatgtcgtc ctgcggcacg
     1141 tagccgagca ccgcgcggta caggtcgtag ttggcgtaga agtcgtcgcc gttgacgcgc
     1201 agcaccccgc gatcggcccg gcgatagccg gcgagcgcgc ccatcaggct cgacttgccg
     1261 gcccccgacg cgccgacgat cgccacgaac tcgcgcggct cgatcaccag cgacacctgg
     1321 tcgaggatca ccttgccctc gggggtcacg cgcgtgaggc cgcgggcctc gatgcgcatc
     1381 gcgccgacct gctcgagctg gtcgagcgac ttgccggtgt aggtgagctt gaacggcccg
     1441 atctggacga cgtcgccgac cttgagcttc tgggtctgca cccgcgcacc ggcgatgaag
     1501 gtcccgttgg tgctgtcgag gtcgacgacc tcgtggccgg tcccggcgcg gcggatctcc
     1561 gcgtgcacgc gcgagacctg caggctcggc agcgtgagat cgcagccctc ccggccgatg
     1621 cggacccgcg gacggtcgag cggcacccgg tgcaccggcg cgggcaccgg cgcctgggcc
     1681 cgcttgccca tgtcctggta gacgaggctg acgaagttgc cggtgacgcg atcgccgatg
     1741 cggatcacgt cgccgtcgtt gaacacgtgg ctgcgcaccc ggttgccgcg gaacaccagt
     1801 ccggtcgtgt tctcgaggtc gacgatccgg tgcccggtgc cgaagggctc gatcttcgcg
     1861 tggtacggcg cgacgcccgg cgccaccacc acgacgtcac agtccgggcg gcggccgacc
     1921 cgcagcgtct tcggcccgag ccggacgtcg ttgatcttgc ggccgcggtc ctcgacgacg
     1981 agatatgtgg tggaggaccg gattaagacc gtgtcctctg cgccgccgct gccc
//
LOCUS       DASWNJ010000913         2119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_613023, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000913 DASWNJ010000000
VERSION     DASWNJ010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613023"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VGB85_15465"
     CDS             <1..391
                     /locus_tag="VGB85_15465"
                     /inference="COORDINATES: protein motif:HMM:NF012800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135482.1"
                     /translation="GPPGIDDLSPEGETRVVEWRRGAVHTHVLRPEHAGLAPVPIAGL
                     AGEDPAGNAAALLRLLDGEPGPYRTSVQYSGALALLVAGDGELAELPGLAARIGEVLD
                     DGSARRVLRELVACSRGSSPEPGPGAS"
     gene            435..1241
                     /gene="trpC"
                     /locus_tag="VGB85_15470"
     CDS             435..1241
                     /gene="trpC"
                     /locus_tag="VGB85_15470"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEY0135483.1"
                     /translation="MTASGARYLDKILAAKRAELATAGATRSSALSDREMLELGARLP
                     RPRDFIAALREGPRPAIIAEFKRASPSAGSLREDADPRQVATLYAAGGATCMSVLTDR
                     HFGGSFEDLRSVRQTVTLPLLCKDFILEKTQIVEAKRVGADCVLLIAAALQPPQMRNL
                     LQFAHQLDMQVLVETRDEHEIDRAMSAGGRLIGVNARDLDTFELDPDRAARLRKYVPP
                     GFTYVAESGVKSVDDAIRLRDAGVDAILIGSYLMQSPNPGETLRQLVLNL"
     gene            1264..1887
                     /locus_tag="VGB85_15475"
     CDS             1264..1887
                     /locus_tag="VGB85_15475"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEY0135484.1"
                     /translation="MAIKLKICGVTRPEDLAACVELGVDAIGINLWSGSRRGLSIEQA
                     RLLLREAPAGGPERVGVFVNPSEDEVRRAHGELGLDLVQVITDGPATAPSLPYMFVIR
                     GTPAIDQVMITTPAPARFLLDAAVPGYGGAGQTTDWSWARAAVQRLSPAAPVWLAGGI
                     TPDNAAAAISAVSPAGLDVASGAEMADARRGEKDRARIAALLAACRR"
     gene            complement(1921..>2119)
                     /locus_tag="VGB85_15480"
     CDS             complement(1921..>2119)
                     /locus_tag="VGB85_15480"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEY0135485.1"
                     /translation="SLAELRAATQLPVVVGFGVRSGADIKALARSADGVVVGSALVEA
                     GSRSAADLGALVTELRAATVR"
BASE COUNT          281 a          785 c          773 g          280 t
ORIGIN      
        1 tggcccgccc ggcatcgacg acctcagccc cgagggcgag acgcgggtcg tcgagtggcg
       61 ccgcggggcc gtgcacacgc acgtgctgcg gcccgagcac gccggcctcg cgccggtgcc
      121 gatcgccggg ctcgcgggcg aagatcccgc gggtaatgcc gcggccctgc tgcgcctcct
      181 cgacggcgag ccggggccct accgcacgtc tgtgcagtat agtggcgcgc tcgcgctgct
      241 ggtcgcggga gacggtgagc tcgccgagct gccagggctg gccgcccgca tcggcgaggt
      301 cctcgacgac ggcagcgccc ggcgcgtgct tcgcgagctg gtcgcgtgca gccgtggcag
      361 ctccccggag cccggtccgg gcgcgtcctg agccgacgcg ttcgcgcggc tgctgcggca
      421 tactggggcg gaccatgacg gcatcgggcg caagatacct cgacaagatc ctggcggcga
      481 agcgggcgga gctggcgacg gccggggcca cgcgatcgtc ggcgctgtcg gatcgggaga
      541 tgctcgagct cggggcccgc ttgccgcgtc cgcgcgactt catcgcggcg ctgcgcgagg
      601 gcccgcggcc ggcgatcatc gccgagttca agcgcgcctc gccctcggcc ggcagcctgc
      661 gcgaggacgc cgatccgcgg caggtcgcca cgctgtacgc ggccggcggc gcgacctgca
      721 tgagcgtgct gaccgaccgc cacttcggcg gcagcttcga ggacctgcgc agcgtccgac
      781 agaccgtcac gctgccgctg ctgtgcaagg acttcatcct cgagaagacg cagatcgtcg
      841 aggccaagcg ggtcggggcc gactgcgtgc tgctgatcgc cgcggccctg caaccgccgc
      901 agatgcgcaa cttgctgcag ttcgcgcacc agctcgacat gcaggtgctc gtcgagaccc
      961 gcgacgagca cgagatcgat cgggcgatgt ccgccggtgg gcgcctgatc ggcgtcaacg
     1021 cccgcgacct cgacaccttc gagctcgacc ccgaccgcgc cgcccgcctg cgcaagtacg
     1081 tcccgcccgg tttcacgtat gtcgccgaga gcggcgtgaa gtcggtcgac gacgcgatcc
     1141 gcctgcgcga cgccggcgtc gacgcgatcc tgatcggcag ttacctcatg caatcgccca
     1201 accccggcga gacgctgcgg cagctggtcc tcaacctgtg agacgatctg tacgaagggg
     1261 ctcgtggcga tcaagctcaa gatctgcggc gtgacccgcc ccgaggacct cgccgcctgc
     1321 gtcgagctcg gggtcgacgc gatcgggatc aacctgtggt ccggctcgcg tcgcgggctg
     1381 tcgatcgagc aggccaggct gttgctgcgc gaggcgcccg ccggcgggcc cgagcgcgtc
     1441 ggcgtgttcg tcaatcccag cgaggacgag gtccgccgcg cgcacggaga gctcggcctc
     1501 gatctcgtcc aggtgatcac cgatggcccg gccacggccc cctcgctgcc atatatgttc
     1561 gtgatccgcg ggacgccggc gatcgatcag gtgatgatca cgacgcccgc gccggcgcgc
     1621 ttcctgctcg acgcggccgt gcccggctac ggcggcgccg ggcagacgac cgactggagc
     1681 tgggcccgcg cggccgtgca gcggctctcg cccgccgcgc cggtgtggct cgccgggggc
     1741 atcacgcccg acaacgcggc ggccgcgatc tcggcggtgt cgcccgcggg gctcgacgtc
     1801 gccagcggcg cggagatggc ggacgcccgc cgcggcgaga aggaccgcgc ccgcatcgcc
     1861 gccttgctgg ccgcctgtcg tcgttgagga catgtcccgc aggacatgtc cccggggaca
     1921 tcaacgcacg gtcgccgcgc gcagctcggt gaccagcgcc ccgaggtcgg ccgcgctgcg
     1981 gctgccggcc tcgacgagcg ccgagccgac gaccacaccg tcggcgctgc gcgccagcgc
     2041 cttgatgtcc gcgccgctgc gcaccccgaa gccgacgacc acgggcaatt gcgtggcggc
     2101 gcgcagctcg gcgagcgag
//
LOCUS       DASWNJ010000914         1660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_264615, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000914 DASWNJ010000000
VERSION     DASWNJ010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264615"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /locus_tag="VGB85_15485"
     CDS             <1..1175
                     /locus_tag="VGB85_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY0135486.1"
                     /translation="DNVAALKQNLKRVGIFLTAVSETNQNLPTAGAGGREVDLSAIFD
                     RISQKLVSRNTRLLATLLCAGVTLPEALQALTDQVESRRFKGVLGDVANKVNEGSSLA
                     DAISRYPDVFLPLYINMVRAGEASGSLETVLLRIADFMDQQEELKGKVTSAMIYPLIM
                     TVLSGGIVMMLMINVVPQITEMFDGMNAELPWNTKLLIYVSDLLASWWFLFIAGAFGV
                     VWLFRRWRKTEAGRATGDTILLKIPVVGDLARKLAIARFARTLATMLASGVQLLQALD
                     IVRSLLGNYVLEKVVGTARENIREGEGIAPALKRSGEFPSLVTHMIAVGERSGQLEQM
                     LIDVANAYDREVNIALTRMTATLEPLMIVLMGGSVGFIVFSIMQPIMLLNEMAGQQ"
     gene            1226..>1660
                     /locus_tag="VGB85_15490"
     CDS             1226..>1660
                     /locus_tag="VGB85_15490"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HEY0135487.1"
                     /translation="MNTLTLIKTDLAARKQLRRRRSRGMTLIEILVVLAIIGLIVGGV
                     SVVAFNAFGDAKIKSGGNDVINVQKACEQYMLQKNDKCPKSMADLKAAGVVSRVQKDP
                     WGEEFVIVCPGEHGPVDVISYGPDKKQGGGDDLVSWEAPKEKE"
BASE COUNT          304 a          582 c          516 g          258 t
ORIGIN      
        1 ccgacaacgt cgccgcgctc aagcagaacc tcaagcgcgt cggaattttc ctgacggcgg
       61 tcagcgagac caaccagaac ctgccgaccg cgggcgcggg cggccgcgag gtcgacctca
      121 gcgcgatctt cgaccgcatc agccaaaagc tcgtgtctcg caacacgcgc ctgctggcga
      181 cgctgctgtg cgccggcgtg accttgcccg aggcgttgca ggccctgacc gaccaggtcg
      241 agagccggcg cttcaagggc gtgctcggcg acgtcgccaa caaggtcaac gagggcagct
      301 cgctggcgga cgccatctcg cgctacccgg acgtgttcct gccgctctac atcaacatgg
      361 tccgcgcggg tgaggcctcg ggctcgctgg agacggtgct gctgcgcatc gccgacttca
      421 tggaccagca agaggagctc aagggcaagg tcacctcggc gatgatctac ccgttgatca
      481 tgaccgtgct cagcggcggc atcgtcatga tgctgatgat caacgtcgtc ccgcagatca
      541 cggagatgtt cgacggcatg aacgccgagc tgccgtggaa caccaagctc ctgatctacg
      601 tcagcgacct cctggcctcc tggtggttcc tgttcatcgc cggcgccttc ggtgtcgtgt
      661 ggctgtttcg ccgctggcgc aagaccgagg ccgggcgcgc caccggcgat accatcctcc
      721 tcaagatccc ggtcgtcggc gacctcgcgc gcaagctggc gatcgcccgc ttcgctcgca
      781 ccctggcgac gatgctcgcc agcggcgtgc agctgctgca ggccctcgac atcgtccgct
      841 cgctgctcgg caactacgtg ctcgagaagg tcgtcggcac cgcccgcgag aacatccgcg
      901 agggcgaggg catcgccccc gcgctcaagc gcagcggcga gttcccctcg ctggtcaccc
      961 acatgatcgc cgtcggcgag cgctccggcc agctcgagca gatgctgatc gacgtcgcca
     1021 acgcctacga ccgcgaggtc aacatcgccc tgacgcgcat gaccgccacc ctcgagccgc
     1081 tcatgatcgt cctgatgggc ggcagcgtcg gctttatcgt attttccatc atgcagccga
     1141 tcatgctgct caacgagatg gccggacagc agtagagccc ccgcggaaga tcgcaccaca
     1201 gctcgcacca agcaggagac agaccatgaa cacgctcact ctcatcaaga ccgacctcgc
     1261 cgcccgcaag cagctccgcc gccgtcgcag ccgcggcatg accctcatcg agatcctggt
     1321 cgtgctcgcg atcatcggcc tcatcgtcgg cggcgtcagc gtcgtcgcgt tcaacgcctt
     1381 cggcgacgcg aagatcaagt ccggcggcaa cgacgtcatc aacgtgcaga aggcctgcga
     1441 gcagtacatg ctgcagaaga acgacaagtg ccccaagagc atggccgacc tcaaggccgc
     1501 gggcgtcgtc tcgcgcgtcc agaaggaccc gtggggcgag gagttcgtga tcgtctgccc
     1561 gggtgagcac ggcccggtcg acgtcatctc ctacggcccc gacaagaagc agggcggcgg
     1621 cgacgacctc gtcagctggg aagccccgaa ggagaaggaa
//
LOCUS       DASWNJ010000915         6142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_475510, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000915 DASWNJ010000000
VERSION     DASWNJ010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6142
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475510"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2970)
                     /locus_tag="VGB85_15495"
     CDS             complement(<1..2970)
                     /locus_tag="VGB85_15495"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0135488.1"
                     /translation="MPLATIPAAVVAMAPNFAQAASDVTISGVVTDAKSGDKISGALV
                     ILQCSCLQGQREMQTNADGLYSFRNLPQGKYTVQVLFGQANVNKSMDVPAGAKFRANF
                     AVDPQNKFRIDVEVQSKVRSDAANVTKVKMDEMRQVPVGGTSRDFTAVLDLSPTASRD
                     AAGVRLAGTSGAESKYVVDGANATNPAFGTVSATIVQEFVEEVEVLEAGYDAEYGGGS
                     GGQVRARRIGGTNKFRGTVLLRFSPRIALPRFVAQTDESLRVAEVRDYEGQGVFTLSG
                     PIIKDKLFFSVAVAPGGQKNSMIQSFFRRRDKDRSGGYEDCPYENGTNDCASNGNYID
                     SVKFAEQKFRTGNWYLQYQGVLDWQITPKHRLRLGGGSAPIFQRSSFRLPPGSEPNSF
                     GTNPAATIGGSSRVAQGVVNDTFGTSFGSSTSASLEYEGRVADDKLEIDAGLYYSRSN
                     TIEAYKLDNKNLKNIPLTQETDAQGRNLYDLLDRDGAVRLVQGVDQACNSASLPGLTC
                     PTRNWLSGGLGSYSKGLADRVGGTFSLTHFLSTKRAGSHQIKYGTEIDHVRQVTDNRY
                     SGANEADFYNNCPAGSKDGGEYCYDPATGYTITTQNRVNNNRAIIVDSDNPDQRTAIG
                     FGRVRAEEGDLRAIATPIGAGIRAPAYNATLTTQNYGIYLQDKWAILSNLYLNVGARW
                     EMQDMKDLLGRRNIFISDNVAPRISLVYDWTDEGKSRLYASYGWFYNQLPVLLNSRAF
                     GGLVNVARTYRQSDCATPVLIGGEARDRTKDGNPTEYCTDFNSSTGGLTAGAVVPRLR
                     GMYRQQFQVGYEQEVVEDLVLGVNWLHTDLGRTVEDISTNGGLNFIIANPGESVSASD
                     IAKQQATCTELQGKFDTAGMDDPQRDVYARELNRCNFLAEAFTKVGTLFNKPVQNYDA
                     WSFRLNKRFAKNWVLQGSYTYSRLVGNYDGFVARNTGAINLGASSQYDIPELVRNAYG
                     PLFANVPHTIKLDGFYSF"
     gene            complement(3452..4300)
                     /gene="murI"
                     /locus_tag="VGB85_15500"
     CDS             complement(3452..4300)
                     /gene="murI"
                     /locus_tag="VGB85_15500"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEY0135489.1"
                     /translation="MIEPRLDRRYAPIGVFDSGVGGLTVLRALREALPHEPLLYLGDT
                     ARLPYGSKSAGTVVRYAEQAARVLVARGVKLLVIACNTASAVALEHLSEVFHPLPVIG
                     VVEPGAEAACAASTSGEIIVIATEGTVRGGAYERAIRALRAGACVVQTPCPLFVALAE
                     EGWTEGPIAEAIAARYLGGVLREHPAADCLVLGCTHFPVLRAAISAVVGSRLTLVDSA
                     ETTARVVAAELAARGLLHPAAAGQAACRFLATDAPARFARVGAVFMGDALAPALTAEA
                     VELVDL"
     gene            complement(4300..5445)
                     /locus_tag="VGB85_15505"
     CDS             complement(4300..5445)
                     /locus_tag="VGB85_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0135490.1"
                     /translation="MFDPTYTEEQQALIETARKFTREVIIPAAHEYDEAEKFPTPVFN
                     AAFELGLMNVELPEAYGGLGLHTLDGCLMAEELAYGCAAMATSIMCNHLGALPLLIAG
                     TEAQKERYLGWLTREPIFASYACSEPEAGSDVAAMKTRLTRDGDGWRLTGQKRWITNA
                     GHAAFFTGFATLDPKLGHKGITGFVVEAERAGISIGKKEKKLGQRASETCDVIFEDVK
                     LTDADLLGEPGKGFYIAMETFDKSRPMIAAQCAGMIRRCMDESAKYAKERKTFGKAIA
                     EHQAVQFMIAEMAMAYETTRLLYSKAAWEVDNNIKRTITSAVAKCTGADLAVKCATDA
                     VQVFGGYGYTREYPVEKIYRDAKLMQIYEGTSQIQRMVIAKNILLRG"
     gene            complement(5471..5674)
                     /locus_tag="VGB85_15510"
     CDS             complement(5471..5674)
                     /locus_tag="VGB85_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135491.1"
                     /translation="MTLGHFLYIPGVLCLGILIGYILGGRAVEVARADREADLRRQAA
                     RRARRDAEAGTPGTPPADNPPAP"
     gene            complement(5691..5993)
                     /locus_tag="VGB85_15515"
     CDS             complement(5691..5993)
                     /locus_tag="VGB85_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135492.1"
                     /translation="MQAPTPTLTPQTQPTTQAPGQPAADPAYGPAVGLSLKEKEQVRQ
                     LEGIAAEKTARDYQHIVLAYGVLWALFAGYGIFLWRRSARLRADMDDLRRTLDRRP"
BASE COUNT          905 a         1956 c         2249 g         1032 t
ORIGIN      
        1 gaaggagtag aagccgtcga gcttgatcgt gtgcgggacg ttggcgaaca gcgggccgta
       61 cgcgttgcgg accagctccg ggatgtcgta ctgcgacgag gcgccgaggt tgatcgcgcc
      121 ggtgttgcgc gcgacaaagc cgtcgtagtt gccgacgagg cgcgagtagg tgtagctgcc
      181 ctggaggacc cagttcttgg cgaagcgctt gttgagccgg aagctccacg cgtcgtagtt
      241 ctggaccggc ttgttgaaca gcgtgccgac cttggtaaag gcctccgcca ggaagttaca
      301 gcggttgagc tcgcgggcgt acacgtcgcg ctgcgggtcg tccatgccgg ccgtgtcgaa
      361 cttgccctgg agctcggtgc acgtcgcctg ctgcttggcg atgtccgacg ccgagaccga
      421 ctcgcccggg ttggcgatga tgaagttgag gccgccgttg gtcgagatgt cctcgaccgt
      481 gcggccgagg tcggtgtgca gccagttgac gccgagcacc aggtcctcga cgacctcctg
      541 ctcgtagccg acctggaact gctggcggta catgccgcgc aggcggggga ccacggcgcc
      601 cgcggtcaga ccgccggtgc tcgagttgaa gtccgtgcag tactcggtcg ggttgccgtc
      661 cttggtccgg tcgcgggcct cgccaccgat cagcaccggc gtcgcacagt ccgactgacg
      721 gtaggtgcgg gcgacgttga cgaggccgcc gaaggcgcgg ctgttgagca gcaccgggag
      781 ctggttgtag aaccagccgt agctggcata caggcggctc ttgccctcgt ccgtccagtc
      841 gtagaccagg ctgatgcgcg gggcgacgtt gtcggagatg aagatgttgc gcctgccgag
      901 caggtccttc atgtcctgca tctcccagcg cgcgccgacg ttgaggtaca ggttggagag
      961 gatcgcccac ttgtcctgaa ggtagatccc gtagttctgg gtcgtcaggg tggcgttgta
     1021 ggccggggcg cggataccgg cgccgatcgg cgtcgcgatc gcgcggaggt caccctcctc
     1081 ggcgcggacg cgaccgaagc cgatcgcggt gcgctggtcg gggttgtccg agtcgacgat
     1141 gatcgcgcgg ttgttgttga cgcggttctg cgtcgtgatc gtgtaaccgg tcgccgggtc
     1201 gtagcagtac tcgccgccgt ccttggagcc cgccggacag ttgttgtaga agtcggcctc
     1261 gttggcgccc gagtagcggt tgtcggtgac ctgacggacg tggtcgatct ccgtgccgta
     1321 cttgatctgg tgcgagccgg cccgcttggt gctcaggaag tgcgtcagcg agaaggtgcc
     1381 gccgacgcgg tccgcgaggc ccttcgagta cgagccgagg ccgcccgaca gccagttgcg
     1441 ggtcggacag gtcaggcccg gcaggctcgc gctgttacac gcctggtcga cgccctggac
     1501 caggcgcacg gcgccgtcgc ggtcgagcag gtcgtagagg ttgcggccct gcgcgtccgt
     1561 ctcctgcgtc agcgggatgt tcttgaggtt cttgttgtcg agcttgtacg cctcgatcgt
     1621 gttcgaccgc gagtagtaca ggccggcgtc gatctcgagc ttgtcgtcgg cgacgcggcc
     1681 ctcgtactcg aggctcgcgc tggtcgagct gccgaagctg gtgccgaagg tgtcgttgac
     1741 gacgccctga gcgacgcgcg agctgccgcc gatcgtcgcg gccgggttgg tgccgaacga
     1801 gttgggctcg ctgcccggcg ggaggcggaa gctgctgcgc tggaagatcg gcgcggagcc
     1861 gccgccgagg cgcaggcggt gcttgggggt gatctgccag tcgagcacgc cctggtactg
     1921 gaggtaccag ttgccggtgc ggaacttctg ctcggcgaac ttgaccgagt cgatgtagtt
     1981 gccgttcgac gcgcagtcgt tggtgccgtt ctcgtacgga cagtcctcgt agccgcccga
     2041 gcggtccttg tcgcggcggc ggaagaacga ctggatcatc gagttcttct gcccgcccgg
     2101 cgcgaccgcg accgagaaga acagcttgtc cttgatgatc gggcccgaga gcgtgaacac
     2161 gccctgaccc tcgtagtcgc ggacctcggc gacgcgcagc gactcgtcgg tctgggcgac
     2221 gaagcgcggc agggcgatgc gcggcgagaa gcgcagcagc acggtgccgc ggaacttgtt
     2281 ggtgccgccg atgcggcgcg ctcgcacctg accgccggag ccgccgccgt actcggcgtc
     2341 gtagccggcc tcgaggacct cgacctcctc gacgaactcc tggacgatcg tcgcggagac
     2401 ggtaccgaag gccgggttgg tcgcgttggc gccgtcgacg acgtacttcg actcggcgcc
     2461 cgaggtgccc gcgaggcgca cgccggccgc gtcacggctc gccgtcggcg acaggtcgag
     2521 cacggcggtg aagtcgcggc tggtgccgcc gaccggcacc tgccgcatct cgtccatctt
     2581 caccttggtc acgttcgcgg cgtcgctgcg gaccttggac tggacctcga cgtcgatgcg
     2641 aaacttgttc tgggggtcga cggcgaagtt ggcgcggaac ttggcgccgg ccggcacgtc
     2701 catcgacttg ttgacgttgg cctgaccgaa caggacctgg accgtgtact tgccctgcgg
     2761 caagttgcga aacgagtaca gaccgtcggc gttggtctgc atctctcgct ggccctgaag
     2821 acaggagcac tggaggatga cgagcgcgcc cgagatcttg tcgccgctct tggcgtccgt
     2881 gacgacgccc gaaatagtca cgtccgaggc cgcctgggcg aagttcggcg ccatggcgac
     2941 gaccgctgcg gggatcgtgg ccaggggcaa agacagcgag taactgagca gagagcggtt
     3001 gaggttcacg ggcagggtgc tccttgcaga cacgacgaca gcggcaaagc ggcggtggta
     3061 gcaggtgtgg cggcaaaaac cggcgggatg ccagcggggt ataacataac gaaacggccg
     3121 ctgaatacgc gaaactgcaa cagtctgccg tgggtccgcg accttgtgac cgggcatgga
     3181 tcggacgtca ggacgtcgcc tcgcatgtcg cctacaacgg cgatccggcg gccgaatcgc
     3241 aagatcctcg aaggacgaag atcaggggta gatcggggat gtccgtcggt cgggcggcgc
     3301 tctcacaggc atgtgctcgc ctgttcgtgg accgatgaac gcagcacggc ggacaaagcc
     3361 acggcgccga agatctttgg gatgctgcgg caaccggacg ggccgagtca acgaaatttg
     3421 tctcggtcac ctcggcgacc gcgggcggcg gtcagaggtc gacgagctcg accgcttcgg
     3481 cggtcagcgc gggggccagc gcgtcgccca tgaacacggc accgacccgg gcaaaccggg
     3541 ccggcgcatc ggtggcgagg aaccggcacg cggcctgccc cgcggccgcc gggtgcagga
     3601 ggccacgcgc ggccagctcg gccgcgacca cgcgggcggt ggtctcggcc gaatccacca
     3661 gcgtcagccg cgagccgacg accgccgaga tcgccgcgcg caggaccggg aagtgggtac
     3721 aaccgaggac caggcagtcg gccgccgggt gctcgcgcag caccccaccg aggtaccgcg
     3781 cggcgatcgc ctcggcgatc ggaccctcgg tccagccctc ctcggccagg gcgacgaaca
     3841 gcggacaggg cgtctgcacg acgcaggccc cggcacgcag cgcccggatc gcccgctcgt
     3901 aggcgccgcc acggacggtg ccctccgtgg cgatgacgat gatctccccc gaagtggaag
     3961 cggcgcaggc ggcctcggcg cccggctcga cgacgccgat gaccggcagg gggtggaaca
     4021 cctcgctgag gtgctcgagc gccaccgccg aggcggtgtt gcaggcgatc accagcagct
     4081 tgacgccgcg ggcgacgagc acgcgggcgg cctgctcggc gtagcggacg acggtgccgg
     4141 cgctcttgct gccgtacggc aggcgcgccg tatcgccgag gtacagcagc ggctcgtgcg
     4201 ggagcgcctc gcgcagcgcc cggagcacgg tgagcccgcc gaccccggag tcgaacacgc
     4261 cgatcggcgc gtatcgtcgg tccaggcgag gctcgatcat cagccgcgca gcaggatgtt
     4321 cttggcgatc accatgcgct ggatctgcga ggtgccctcg tagatctgca tcagcttggc
     4381 gtcgcggtag atcttctcga ccgggtactc gcgggtgtag ccgtagccgc cgaacacctg
     4441 cacggcatcg gtcgcgcact tgaccgccag gtcggcgccg gtgcacttgg cgaccgcgct
     4501 ggtgatcgtg cgcttgatgt tgttgtcgac ctcccacgcg gccttgctgt acagcaggcg
     4561 ggtggtctcg taggccatcg ccatctcggc gatcatgaac tgcacggcct ggtgctcggc
     4621 gatcgccttg ccgaaggtct tgcgctcctt ggcgtacttc gccgactcgt ccatgcaccg
     4681 gcggatcatg ccggcgcact gcgccgcgat catcggccgc gacttatcaa aggtctccat
     4741 ggcgatgtag aagcccttgc ccggctcgcc gaggaggtcg gcgtcggtga gcttgacgtc
     4801 ctcgaagatc acgtcgcagg tctccgaggc gcgctggccg agcttcttct ccttcttgcc
     4861 gatgctgatc cctgcgcgct cggcctcgac gacgaagccg gtgatgccct tgtggccgag
     4921 cttggggtcg agggtggcga agccggtgaa gaacgcggcg tggccggcgt tggtgatcca
     4981 gcgcttttgc ccggtcagcc gccagccgtc gccgtcgcgc gtcaggcggg tcttcatcgc
     5041 cgcgacgtcg ctgccggcct cgggctcgga gcaggcgtag ctggcgaaga tcggctcgcg
     5101 cgtcagccag ccgaggtagc gctccttctg agcctcggtg ccggcgatca gcagcggcaa
     5161 ggcgccgagg tggttgcaca tgatgctcgt cgccatcgcc gcgcagccgt acgcgagctc
     5221 ctcggccatc agacatccgt cgagggtgtg caggccgagg ccgccgtacg cctccggcag
     5281 ctcgacgttc atcaggccga gctcgaacgc ggcgttgaac accggcgtcg ggaacttctc
     5341 ggcctcgtcg tactcgtgcg ccgcggggat gatgacctca cgcgtgaact tgcgtgcggt
     5401 ctcgatgagg gcctgctgct cttcggtata ggtggggtcg aacatggcgt ctcgcgcggg
     5461 ctcccgagta tcacggggcc ggagggttgt cagcgggggg cgtgcccggg gtgcctgcct
     5521 cggcgtcccg tcgggcgcgc cgcgcggcct gacggcgcaa gtcggcctcc cggtcggcgc
     5581 gggcgacctc gacggcgcgc ccgccgagga tgtacccgat gaggatcccc aggcacagca
     5641 cgcctggaat gtagagaaaa tgcccgaggg tcatcgctgg ctcgcggtgc tcatggacgg
     5701 cggtcgaggg tgcggcgcag gtcgtccatg tcggcgcgaa ggcgcgccga ccgacgccag
     5761 aggaagatac cgtagccggc gaacagcgcc cacaacactc cgtacgcgag cacgatgtgc
     5821 tgatagtccc gcgcggtctt ctcggcggcg atgccctcga gctggcgcac ctgctccttc
     5881 tccttcaacg acagaccgac ggcggggccg tacgcggggt ctgccgccgg ttgccctggg
     5941 gcctgcgtgg tgggttgcgt ctgcggggtg agcgtcggcg tgggtgcttg catgagtggt
     6001 cctgctggtc agacgcgggg gggttcaatc ggtcgcgggt tcacgaacgc ggggtgctcg
     6061 gatgccttgt cgcgtgttcg cggacgcggg gtgctcggat gccttggtcg cgtgttcgcg
     6121 gacgcggggt gctcggaggc ct
//
LOCUS       DASWNJ010000916         2162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_475539, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000916 DASWNJ010000000
VERSION     DASWNJ010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475539"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(259..1275)
                     /locus_tag="VGB85_15520"
     CDS             complement(259..1275)
                     /locus_tag="VGB85_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135493.1"
                     /translation="MLRLALAWHLARAQVPEPPDLPIAGIEWSAPSGCPGRADLLAAI
                     SRRLGRELAPDELALDARVVRVGSRHALRLRVVVGDRSELRELDAEACTPLVDATALL
                     VVHTLAEVASEPAPTDREPLPPVVDAPVPDVAPAPVAAPPPAIAPPPVDPPRVASPSP
                     APRPRGPGVLLRAHGGPELGAAPRWTGLAGLAVGLLWPRLRLEARASFLGPRSVTRVD
                     TEVTALLLAGSLHACARLGRGALELPLCAGLEAGGMRGAAYGPAAGRSAFMPWLGVVL
                     GVGAAWRVRPRLALWSALEVVGGVVRPNFVLRGRESDIALFRPFPLSGRLLVGLELRL
                     RDPR"
     gene            1486..>2162
                     /locus_tag="VGB85_15525"
     CDS             1486..>2162
                     /locus_tag="VGB85_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0135494.1"
                     /translation="MPPGPRSIAPEPDLAGTSTVACTDARAGARTSVRRFSLAVVEGP
                     AVGVTWHSTGDRCSIGSHPRNDLVVEDPTVSRFHCEIVIDDGGARVRDLQSRNGTVLD
                     GVTVREAFLRAGSLLRLGKTVLRFDLALELNRLPLSESTHFGTLTGVSAAMRAAFAML
                     ARAADSDATVLLEGETGTGKSQAARSIHREGPRREGPFVVVDCGAILGNLLESELFGH
                     EKGAFTGA"
BASE COUNT          307 a          774 c          839 g          242 t
ORIGIN      
        1 acgcccgcga acagcccgcg cggcccggtg tgaaacagca gcggctcgtc gtcgacgagc
       61 agatactggt tgaagttgaa gccgggcacc ttcgtgttga gccggtagat cccgtcggcg
      121 acctcgtcga tccgcgtctg catcgcacac cccggcgcga ctataccagc cgatgacagc
      181 cgacccgcgg cgcacggcgt gaaggcgatc cgccgggtgc gatcgtactt gcggccgagg
      241 acagcgcacc gcgcagcttc accggggatc gcggagccgc agctcgagac cgacgagcag
      301 ccgccccgac agcgggaacg ggcggaacag cgcgatgtcg gactcgcggc cgcgtagcac
      361 gaagttcggg cgcacgaccc cgccgaccac ctcgagcgcc gaccacagcg ccagccgcgg
      421 gcgcactcgc cacgccgcgc cgaccccgag cacgaccccg agccacggca tgaaggcgga
      481 ccgacccgcg gcgggcccgt acgccgcgcc acgcatgcca ccggcctcga ggcccgcgca
      541 cagcggcagc tcgagcgcgc cgcgaccgag gcgcgcgcac gcgtgcagcg agccggcgag
      601 cagcagcgcg gtgacctcgg tgtcgacgcg ggtgacgctg cgcgggccga ggaagctcgc
      661 gcgggcctcg aggcgcagcc gcggccacag cagtccgacg gcgagcccgg cgagcccggt
      721 ccaccgcggc gccgcgccga gctcggggcc gccgtgagcc cgcagcagca cgcccgggcc
      781 gcgcggacgc ggggcggggg agggtgacgc gacgcgcggc ggatcgaccg gcggcggtgc
      841 gatcgcaggc ggcggcgcgg cgactggcgc gggcgcgacg tccgggaccg gcgcgtcgac
      901 gacgggcggc agcggctcgc ggtcggtggg agctggctcg ctggcgacct ctgcgagggt
      961 gtgcacgacg agcagggcgg tcgcgtcgac caggggcgtg caggcctccg cgtcgagctc
     1021 gcgcagctcg ctgcgatcgc cgacgacgac gcgcagacgc agcgcgtggc gcgagccgac
     1081 gcgcacgacc cgggcgtcga gcgcgagctc gtcgggcgcg agctcgcggc cgaggcggcg
     1141 ggagatcgca gcgagcaggt cggcgcgccc gggacagccc gacggtgcgc tccactcgat
     1201 gcccgcgatc gggaggtcgg gaggctccgg cacctgggcg cgcgccagat gccacgcgag
     1261 ggcgaggcgc agcaacgcga cgacactagc acgggccgcc gctggcgcgc gtcactcgcg
     1321 atcgcgtgct cgaacagctc gcggtcgtat cgacgatcat gcgcggcgct cgtatgtgcc
     1381 gagattgccg atcgaaagcg ccccgctggt atggtgcccg cgacacccat tcgtcatcac
     1441 ggtggcgacg ttgctgcgct cggagattgg ttgtatgcgt acgccgtgcc gcccggacca
     1501 cgcagcattg cgccggagcc ggatctcgcg gggacgtcga cggtcgcctg caccgatgcg
     1561 cgcgcggggg cgcggacgtc ggtgcgccgc ttctcgctcg ccgtcgtcga gggtccggcc
     1621 gtcggcgtga cctggcactc gaccggcgac cgctgctcga tcggctcgca cccgcgaaac
     1681 gacctcgtcg tcgaggaccc gacggtgtcg cggttccact gcgagatcgt gatcgacgac
     1741 ggcggcgcgc gcgtgcgcga cctgcagagc cgcaacggca cggtgctcga cggcgtcacg
     1801 gtgcgcgagg cgttcctgcg cgcgggcagc ttgctgcggc tcggcaagac ggtcctgcgc
     1861 ttcgacctcg cgctcgagct caaccgcctg ccgctatccg agtcgacgca cttcggcacg
     1921 ttgaccgggg tgtcggcggc gatgcgggcg gcgttcgcga tgctcgcgcg ggcggccgac
     1981 agcgacgcga cggtgctgct cgagggcgag acggggaccg gcaagagcca ggcggcgcgg
     2041 tcgatccacc gcgaggggcc gcgacgcgag gggccgttcg tggtcgtcga ctgcggggcg
     2101 atcctcggca acttgctcga gagcgagctt ttcggccacg agaagggtgc gttcaccggc
     2161 gc
//
LOCUS       DASWNJ010000917         2038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_475552, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000917 DASWNJ010000000
VERSION     DASWNJ010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475552"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VGB85_15530"
     CDS             <1..463
                     /locus_tag="VGB85_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135495.1"
                     /translation="IDHPRAALYRGHVLAGLKDIYTAVARERPVPTPLASVLRVTTIR
                     AWDRLVVVPRFGFVSVGDYYDRASVGPRLAQAQIPTLVLSARADPMIPAATIAPYVQH
                     LPPHVTHRWIDRGGHVGFPADLRLDLETTEAGTPAGPGLAAQALAWLRRQP"
     gene            497..1687
                     /locus_tag="VGB85_15535"
     CDS             497..1687
                     /locus_tag="VGB85_15535"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY0135496.1"
                     /translation="MTATASASASPQAEPVSAQCPACSLHALVPVHLEQTAPHRWRQR
                     DAPVASGMTLDTCPVCFGVWLDAGEMDMLGDAEIDPAFLRTLVGQSANRMCPRGHGFM
                     SEHLLPGMLSTPIDRCPRCKGLWLDGEERHALAKSTTREGQEDAKTQLAKRGLIWAAQ
                     LLTHLPIEVENPARGTPWAVYSMCALLVMMYVLSVEGVLFAREWAIVAGHITREPIHA
                     YTIFTHAFFHADWFHLLGNLYFLYVFGDNVEHLFGRRRFLLLYLGAALIGGILQVLLT
                     RATSTPVIGASGAIAGVTAAYLWIFPRARLLQTLPLVYIQLKIPAWVYLGVWIGFQGV
                     MAVFSDAIQFAWFAHIGGFLYGLALTPWVLRWRRRVVASQVRYKSAAAIFGKLPAPRR
                     GGRR"
BASE COUNT          263 a          771 c          667 g          337 t
ORIGIN      
        1 gatcgaccac ccgcgcgccg cgctgtaccg cggccacgtg ctcgccggcc tcaaggacat
       61 ctacacggcc gtcgcccgcg agcgcccggt cccgacgccg ctggcgagcg tgctccgcgt
      121 gacgacgatc cgcgcctggg accgcctggt ggtcgtgccg cgcttcggct tcgtcagcgt
      181 cggcgactac tacgaccgcg cgagcgtcgg cccccgactg gcgcaggcgc agatccccac
      241 gctggtgctc tccgcccgcg ccgacccgat gatccccgca gccacgatcg caccgtacgt
      301 gcaacacctg ccgccccacg tcacccaccg ctggatcgat cgcggcggtc acgtcggctt
      361 ccccgcggac ctgcgcctcg acctcgagac caccgaggcc ggcaccccgg cgggccccgg
      421 cctcgccgcc caggcgctcg cgtggctgcg ccgccagccc tgacccgccg cacacaactg
      481 gtctatcctc gcagccgtga ccgcgaccgc cagcgcgagt gcgtccccgc aggcggagcc
      541 ggtgtccgcc cagtgcccgg cgtgcagcct gcacgcgctg gtccccgtgc acctcgagca
      601 gaccgcaccg caccgctggc gccagcgcga cgcgccggtc gcgtccggca tgaccctcga
      661 cacctgcccg gtgtgcttcg gcgtgtggct cgacgccggc gagatggaca tgctcggcga
      721 cgccgagatc gacccggcgt tcttgcgcac gctcgtcggc cagagcgcca accgcatgtg
      781 cccccgcggc cacggcttca tgtcggagca cctgctgccc ggcatgctga gcacaccgat
      841 cgaccgctgc ccgcgctgca agggcctgtg gctcgacggc gaggagcgcc acgcgctcgc
      901 caagtcgacg acccgcgagg gccaggaaga cgccaagacg cagctcgcca agcgcggcct
      961 gatctgggcg gcgcagctgc tgacgcacct gccgatcgag gtcgaaaacc ccgcccgcgg
     1021 caccccctgg gcggtctaca gcatgtgcgc gctgctcgtc atgatgtacg tgctctcggt
     1081 cgagggcgtg ctgttcgccc gcgagtgggc gatcgtcgcc ggccacatca cccgcgagcc
     1141 gatccacgcc tacacgatct tcacccacgc gttcttccac gccgactggt tccacctgct
     1201 cggcaacctc tactttttat acgtgttcgg cgacaacgtc gaacacctgt tcggccgccg
     1261 ccgcttcctc ctgctctacc tcggcgccgc cctcatcggc ggcatcctgc aagtcctcct
     1321 cacccgcgcc acctccaccc ccgtcatcgg cgcgtcgggt gcgatcgccg gcgtgaccgc
     1381 agcctacctg tggatcttcc cccgcgcccg cctgctgcag accctgcccc tcgtgtacat
     1441 ccagctcaag atcccggcct gggtctacct cggggtctgg atcggctttc agggggtcat
     1501 ggcggtgttc tcggacgcga tccagttcgc ctggttcgcg catatcggtg gatttttata
     1561 tgggctcgcg ctgacaccgt gggtgctccg ctggcgtcgt cgggtggtcg cttcgcaggt
     1621 gcggtataaa tcggcggcgg cgatcttcgg gaagctgccg gcgcctcggc gggggggtcg
     1681 tcggtgagtc ggtgcttggg cgtcgtcgcg tgctcggacg cgtcattgcg tgctcggacg
     1741 cgtcatcgcg tgctcgggtg atgcggcgtg ttcggatgcg tcatcgcgtg ctcggagacg
     1801 cggagtgctc gaacgcttca tcgcgtgctc gggtgatgcg gagggttcga acgcgtcatc
     1861 gcgtgctcgg agacgcgggg cgaggcttca tgggccgggg ggggccgacg gacagcgggt
     1921 ccctgcgacg ctgcgacgcg ggtcctccca ggggacatgc tcgccgcccc atctgtcgcg
     1981 cataacatct cgcttacgcc aagattgtct ctcacgaccg gaagttgacg gggcgtct
//
LOCUS       DASWNJ010000918         8940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_399383, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000918 DASWNJ010000000
VERSION     DASWNJ010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8940
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399383"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VGB85_15540"
     CDS             complement(<1..596)
                     /locus_tag="VGB85_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135497.1"
                     /translation="MNFSTYSRVVGPVNPGKTVALATILALTGCPGGGETTTTTSSTT
                     DVGTTTGTTGDVEPTGGTTGDTSTTDAPTSSSTTGSTTDPASTTDVSTSTTDVSTSTT
                     DASTSTTDTTGGSSSTGADAVCGDGEVEQDEACDDANAGLGDGCTAECTRECTALHFE
                     GKTVVKAANPATLSPVSVTAAAWHKAAANAPYGAIVSKA"
     gene            complement(794..2431)
                     /locus_tag="VGB85_15545"
     CDS             complement(794..2431)
                     /locus_tag="VGB85_15545"
                     /inference="COORDINATES: protein motif:HMM:NF019291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HEY0135498.1"
                     /translation="MFTRPEVVAALLDLAGYTIEQPLHTWRLLEPSFGAGDVLLAAVE
                     RLLASFTAAGGSPKRGDELRDAIRAVEVHPASFVGVRAALRNLLIGRWGAPPAVADAL
                     CDHWLVCDDFLLAPIAGTFEIVVGNPPYVRQERIPGPLLAEYRRRYSTVYDRADLYVP
                     FFERGLQLLQEHGRLAFICANRWLKNKYGGPLRALIARDFHLSHYIDMEGTDAFKKKD
                     VNAYPAITVLRRGVGVMTRIGRRPEAIHALPGLIEAMVAPGPVHDERVVEVEGAVQGS
                     EPWLLDAAAPLRVLRRLEQDFLKLEDCGCTVGIGVATGADGVFIGDDAALPVEEARKL
                     PLVMASDLRGGEIAWGGRFVLNPFEVDGRVAILERYPRFLAYVEAHREILKRRHVARR
                     SGEGWYRTIDRVHVGLTYTPKLLIPDIKGEPVVVLDEGRYYPHHNLYHVTSSRWDLKA
                     LATILRSSVAVLFVSTYCVKISGGFLRFQAQYLRRIRLPRWESLTVEQRAALRAACPT
                     DRAAIDHAVFAAYGLTERESVIVREAAGAALVQGNER"
     gene            complement(2503..4026)
                     /locus_tag="VGB85_15550"
     CDS             complement(2503..4026)
                     /locus_tag="VGB85_15550"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0135499.1"
                     /translation="MRNAIYTAALLLVACRPATTTAPQTDNAPPPQTGADGQPVQTMT
                     WPDEPYRAQAPAAAPIAELKLPSVETFKLANGLDVYLVPQTKLPTVTMSFEFDMGGVD
                     DPKAKTGMTSICMDLLDEGTKKYETAAFEEKQADHAINVSSFGSGESSGINVRALKSQ
                     LGPALDLMAEMLQEPGMRQTDLDRIKERRKASLLQSKASPGPIAGRLFGALVWGENHP
                     YGRIETEKTIDAVSLADCAKVVGKLRPGGARLWVTGMITQDELKEALGSRLPAWKGKA
                     PAKAKIAAAKPRLGTVFFVHVKDAAQSSIYVGHPGPRRTDPDYEATDLMMEILGGSFS
                     SRINMNLREDKGYTYGGRAGIGYRRAGGSFAASSSVRTDVTGPALREVIKEIVTMRAG
                     DPTPEELRRVQEGALLALPAEFATPSDTLFAFQRLKFYDLPLDWYAGFQQRVRAVDTA
                     AVRKAAEKHLRAQDFVVLVVGDASKVLPDLDAIATEKLFGKGGVVVLDADGKPTARP"
     gene            complement(4035..5525)
                     /locus_tag="VGB85_15555"
     CDS             complement(4035..5525)
                     /locus_tag="VGB85_15555"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0135500.1"
                     /translation="MARHALLPLTLIFGALTIPAAYAAPATPAPTTTAASAAAAPATA
                     PATAPATAPAINIEYERFTLDNGLEVILHRDPSVPLVATNVWYHVGSGDETPGKSGFA
                     HLFEHMMFQGAKHIGKDVHFKILQEIGGTSVNGTTNSDRTNYFEVVPSHQLETALWLE
                     SDRMGYLLDLLDEASLKNQQEVVRNERRQRYDNVPYGRDRFATAEALYPEGHPYRYMT
                     IGRHEDLEGAAIEDVKNFFRQWYVPSNATLTLAGDFEVAKAKELVTKWFGAFPRTAKP
                     ARKTATTPTLAATVRKDVDDPFARLTRIHYAWHSPPIFTDADFELDVVSTVLGAYGWG
                     RLHRALVLDERSAQSVSVYNAGAQHSSVFHIVVDLKPGQDPVKAEQVIARELDKIRKE
                     PISDAEMKRVLIGTESSFIWGLEDLMGRVEQLQSFNHYTGDPGYAQTYLKRLRALTPA
                     RVRDTAAQILGKPRAEIITKPAAPAGGPPAGTKSDAKAAPKAATKG"
     gene            5819..6295
                     /locus_tag="VGB85_15560"
     CDS             5819..6295
                     /locus_tag="VGB85_15560"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HEY0135501.1"
                     /translation="MLASLGEPGPPLRRWQWIVVHHTAAEHATLPGIDRYHRNHFGDP
                     LGAEYHFIINNGKKDRANRPIGLIDVARWKYQEPAWHLFKPDNAPDSIAICLVGNFEK
                     RALPAEMLASLTELTRALMQLCEIPAERVSTHRVVDEKMTQCPGKLFPREAFLKGL"
     gene            complement(6297..6740)
                     /locus_tag="VGB85_15565"
     CDS             complement(6297..6740)
                     /locus_tag="VGB85_15565"
                     /inference="COORDINATES: protein motif:HMM:NF037735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD domain-containing protein"
                     /protein_id="HEY0135502.1"
                     /translation="MPRKVIFLDIDGVMNSINTRPQHSRGLADYLDPRNVSVLNAIVE
                     ATGAVVVVSSTWRLSTSFAGLKASFAEAGCVAEIVDITPDIDGRQRVLEVAAWLERQA
                     TPPARFVVLDDDYDMPAFPDKLVRTSKQYGLSARDLPAVLAILAD"
     gene            complement(6766..7296)
                     /locus_tag="VGB85_15570"
     CDS             complement(6766..7296)
                     /locus_tag="VGB85_15570"
                     /inference="COORDINATES: protein motif:HMM:NF021295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135503.1"
                     /translation="MSDHLYHPFYCEENAYHLCTHPDLAQRDPAAVFIRGRGDACVIW
                     HQRAAEAPGEPVFWDYHVVVLAQRPWEIWDLDTTLGCPVPAVQYLRRSFRPEVVLPPE
                     LRPRFRVVPAELLRATFASDRSHMRGPDGAFREPPPPWPPIGTGSTLARFLAQEDETL
                     GEELGLPAFLRRVSDR"
     gene            complement(7293..>8940)
                     /locus_tag="VGB85_15575"
     CDS             complement(7293..>8940)
                     /locus_tag="VGB85_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135504.1"
                     /translation="PDSGTTSGTEPTGTTGTTTEPTGDTGVTTTETGEPPGSFWHIEP
                     PVGDGRTWLVSPTGEKTWMLGVNTVMRGKECDGIDQWIKRFEPTRTARIEWARLANGK
                     SGDETVEEPYCFNSVGAFSNINDFDDTPGDSYMIRPFAEGGAEAPYSVVVNIGPGGDD
                     RALRDAKGVVLESGVASARFGDPFNPLFLADIDELAQGQIAERKNDPNLQIWYLGNEI
                     GVFDKGAKTPGVRDYRRYLWSDCAADSTIDAPKCARHVLAGFLRERYADLPALNKAWE
                     SSYPGADFSTIVDVGPRPIPYVSDCNQQCRVDLQVFVHDHLLKTWVEVITTRVRAADP
                     NHIISAPRMAIGASTHFRFWTPASEPGADVWVEDGSTPVPTDTAEVKYNPLDLLRRNG
                     PSGFDLVAFNIYTGDAEFEKPWFTDGVHLIEEKSGLPIIISEFSVRARIDGWTNKGGA
                     GSFVPTNDGVGDQIQRGAYYQSQLEQFYGFKAIIGANWHAWSDRFLETDDAHQINMGL
                     FRCEVPKSGHKAGERWPEIDERIAAVNCDIMNLIEAKTGL"
BASE COUNT         1323 a         3052 c         3070 g         1495 t
ORIGIN      
        1 gccttgctga cgatcgcacc gtagggggcg ttcgccgccg ccttgtgcca cgccgccgcc
       61 gtcaccgaca ccgggctcag cgtcgccggg ttggccgcct tcaccaccgt cttgccctcg
      121 aagtgcagcg ccgtgcactc cctggtgcac tccgccgtac aaccatcgcc gagacccgcg
      181 ttggcgtcgt cgcacgcctc gtcctgctcg acctcgccgt cgccgcacac cgcgtccgcg
      241 ccggtgctgc tcgaaccccc ggtcgtgtcg gtcgtgctcg tcgaagcatc cgtcgtgctc
      301 gtcgaaacat cggtcgtgct cgtcgaaaca tcggtcgtgc tcgccgggtc cgtcgtcgaa
      361 ccggtcgtgc tgctcgacgt cggcgcgtcc gtggtgctgg tgtcgccggt cgtgccgccc
      421 gtcggctcga cgtcacccgt cgtgccggtc gtcgtgccga catcggtggt gctcgatgtc
      481 gtcgtcgtgg tctcaccgcc gcccggacag ccagtgagcg ccaggatcgt cgccagcgcg
      541 accgtcttgc cgggattgac gggaccgacg acccgagagt acgtggagaa gttcatggca
      601 atttcctcgt gcacaaaact acgtatatcg aggcgaacac gagggaacgc ttccctcgcc
      661 cacatcgacg tgaatcgccg aatctcacga cgccgacggc cctgtcaagc cgcatattcg
      721 ccgctgtaac ggcgtgggcg cccgcccagc gcccgcccag cgcccttacg gacccgcggt
      781 gtcacagtgc acgctaacgc tcgtttccct gcacgagcgc cgcgccggcg gcctcccgga
      841 cgatgacgct ctccctctcc gtcaatccat acgcggcgaa caccgcgtga tcgatcgccg
      901 cacgatcggt cgggcaagcc gcgcgcagcg ccgcacgctg ctccaccgtc agcgactccc
      961 agcgcggcag gcggatcctc cgcaggtact gcgcctgaaa ccgcaaaaaa ccgcccgata
     1021 tctttacaca atacgtcgag acgaacagca cggcgaccga cgaccgcagg atcgtcgcca
     1081 gggccttcag gtcccagcgt gaggaggtca catgataaag gttatgatgc ggatagtacc
     1141 gaccttcatc cagcacgacc accggctcgc ccttgatgtc ggggatcagc agcttgggcg
     1201 tatatgtcag cccgacatgg acgcgatcga tcgtgcgata ccagccctcg ccgctcctgc
     1261 gcgcgacgtg acgccgcttc aaaatttccc gatgcgcctc gacatacgcc aaaaacctcg
     1321 gatatctttc caggatcgcg acccggccat ccacctcgaa gggatttaat acaaatctcc
     1381 cgccccacgc gatctcacca cctcgcaggt cgctggccat caccagcggc agctttcgcg
     1441 cctcctcgac cggcagcgca gcgtcgtcgc caataaacac cccatccgcc ccggtcgcca
     1501 cgccgatccc gaccgtgcag ccacagtcct ccaactttaa aaaatcttgc tcgaggcgtc
     1561 ggaggacgcg cagcggggcg gccgcgtcga gcagccacgg ctcgctgccc tgcaccgcgc
     1621 cctcgacctc caccacacgc tcgtcgtgca ccggccccgg agcgaccatc gcttcgatca
     1681 acccgggcag cgcatgtatg gcctcggggc gtcgcccgat gcgggtcatg acgccaacac
     1741 cgcgacggag caccgtgatc gccggatagg cgttgacgtc cttcttttta aaagcgtcgg
     1801 tgccctccat gtcgatgtag tgagataaat ggaagtcgcg ggcgatgagg gcgcgcagcg
     1861 ggccgccgta cttgttcttg agccaccgat tggcgcagat gaaggcgagg cggccgtgct
     1921 cttgcaggag ctgcaggccg cgctcgaaga acggcacata gaggtccgcg cggtcgtaga
     1981 cggtcgaata gcggcggcgg tactctgcca gcagcggccc ggggatccgc tcctgacgca
     2041 cgtagggcgg gttgccgacg acaatttcga acgtgcctgc gatcggggcg agcaggaagt
     2101 cgtcgcacac gagccagtgg tcgcacagcg cgtccgcgac ggccggtggg gcgccccagc
     2161 gccctatcaa caaatttcgc agcgccgcgc gcacgcccac gaacgaggcc ggatggacct
     2221 cgaccgcccg gatcgcgtcg cgcagctcgt cgccgcgctt cggcgagcct ccggcggccg
     2281 tgaagctcgc cagcaggcgc tcgaccgccg ccagcaggac gtcgccggcg ccgaacgagg
     2341 gctcgagcag gcgccacgtg tgcagcggct gctcgatcgt atagcccgcg aggtcgagca
     2401 gggccgcgac gacctccggg cgggtgaaca cggcgccgcg cgccgtcctg tctgcgccac
     2461 ggtccgcccc gtcgtcgccg gcggggcttt gacctgctgg cttcacgggc gcgcggtcgg
     2521 cttgccgtcg gcgtcgagca cgacgacgcc gcccttgccg aacagcttct ccgtggcgat
     2581 cgcgtcgagg tcggggagga ccttgctggc gtcgccgacg acgagcacca cgaagtcctg
     2641 ggcgcgcagg tgcttctcgg cggccttgcg gaccgcggcg gtgtcgacgg cgcgcacgcg
     2701 ctgctggaag ccggcgtacc agtcgagcgg caggtcgtag aacttgagcc gctggaacgc
     2761 gaacagcgtg tccgagggcg tggcgaactc ggccggcagg gcgagcaggg cgccctcttg
     2821 cacgcggcgc agctcctccg gggtcgggtc tccggcgcgc atcgtaacga tctccttgat
     2881 gacctcgcgc agggcggggc cggtgacgtc ggtgcggacc gaggagctcg cggcgaagct
     2941 gccaccggcg cggcggtagc cgatgccggc gcggccgccg taggtgtagc ccttgtcctc
     3001 gcgcaggttc atgttgatcc ggctcgagaa cgatccgccg aggatctcca tcatcaggtc
     3061 ggtcgcctcg tagtcggggt cggtccggcg cgggccgggg tgaccgacgt agatcgacga
     3121 ctgcgcggcg tccttgacgt gcacgaagaa cacggtgccc aggcgcggct tggcggcggc
     3181 gatcttcgcc ttcgcgggcg ccttgccctt ccacgccggc agtcgcgagc cgagcgcctc
     3241 cttgagctcg tcctgcgtga tcataccggt gacccacagg cgcgcgccgc cgggcctcag
     3301 cttgccgacg accttcgcgc agtcggcgag gctgaccgcg tcgatcgtct tctcggtctc
     3361 gatgcggccg taggggtggt tctcacccca caccagcgcg ccgaacaggc gccccgcgat
     3421 cgggccgggg ctggccttgg actgcagcag cgaggccttg cggcgctcct tgatgcggtc
     3481 gaggtcggtc tggcgcatgc cgggctcttg cagcatctcg gccatcaggt cgagcgccgg
     3541 tccgagctgc gacttgagcg cgcggacgtt gatgcccgag ctctcgccgc tgccgaacga
     3601 cgacacattg atcgcgtggt cggcctgctt ctcctcgaag gcggccgtct cgtacttctt
     3661 ggtcccctcg tcgagcaggt ccatgcagat cgacgtcatg ccggtcttgg ctttggggtc
     3721 gtcgacgccg cccatgtcga actcgaacga catcgtcacc gtcggcagct tggtctgcgg
     3781 cacgaggtag acgtcgaggc cgttggcgag cttgaaggtc tcgacgctcg gcagcttgag
     3841 ctcggcgatc ggcgcggccg cgggcgcctg ggcccggtac ggctcgtcgg gccaggtcat
     3901 cgtctggacc ggctggccgt cggcgccggt ctgcggcggc ggcgcgttgt cggtttgcgg
     3961 ggccgtggtc gtcgccgggc ggcaggcgac cagcagcagg gcagcggtat agatggcgtt
     4021 gcgcatggtc gtggtcatcc cttcgtggcg gccttcggcg cagccttggc gtcggacttc
     4081 gtgcctgccg ggggtccgcc cgcgggggca gctggcttgg taataatctc ggcgcgcggc
     4141 ttgccgagga tctgcgcggc ggtgtcgcgg acacgcgccg gcgtcagcgc gcgcaggcgc
     4201 ttgaggtagg tctgggcgta gccggggtcg ccggtgtagt gattgaacga ctggagctgc
     4261 tcgacgcggc ccatcaggtc ctcgagtccc cagatgaagc tcgactcggt gccgatcagc
     4321 acgcgcttca tctccgcgtc ggagatcggc tccttgcgga tcttgtcgag ctcgcgggcg
     4381 atcacctgct cggccttgac cgggtcctgg ccaggcttga ggtcgacgac gatgtggaac
     4441 acgctcgagt gctgcgcgcc ggcgttgtac accgatacgc tctgggcgct tcgctcgtcg
     4501 aggaccaaag cgcggtgcag gcggccccag ccgtaggcgc cgagcacggt cgacacgacg
     4561 tcgagctcga agtcggcgtc ggtgaagatc ggcgggctgt gccacgcgta gtggatccgc
     4621 gtcaggcggg cgaaggggtc gtcgacgtcc ttgcgcaccg tcgccgccag cgtcggcgtc
     4681 gtcgcggtct tgcgcgccgg cttggcggtc ctcgggaacg cgccgaacca cttcgtgacc
     4741 agttccttgg cctttgcgac ctcgaagtcg ccggcgagcg tcagcgtcgc gttgctcggc
     4801 acgtaccact ggcgaaagaa attcttcacg tcctcgatcg ctgcgccctc gaggtcctcg
     4861 tggcggccga tcgtcatgta acgatagggg tggccctcgg ggtacagcgc ctcggcggtg
     4921 gcgaagcggt cacgcccgta cgggacgttg tcgtagcgct ggcgccgctc gttgcggacg
     4981 acctcctgct gatttttcag gctggcctcg tcgagcaggt cgagcagata ccccatgcgg
     5041 tcgctctcga gccacagcgc cgtctcgagc tgatgcgagg gcacgacctc gaagtagttg
     5101 gtgcgatccg aattggtggt accgttgacc gaggtaccgc cgatctcctg caagatcttg
     5161 aagtgcacgt ccttgccgat gtgcttggcc ccctggaaca tcatgtgctc gaacaggtgg
     5221 gcaaagccgc tcttgccggg cgtctcgtcg ccgctgccga cgtgatacca gacgttcgtc
     5281 gccaccaagg gcaccgatgg atcgcggtgc aggatcacct cgaggccgtt gtcgagggtg
     5341 aagcgctcgt actcgatgtt gatcgcgggc gcagtggcag gcgcagtggc aggcgcagtg
     5401 gcaggcgcag cggccgcgct ggccgctgtg gtcgtgggag ccggcgtcgc cggagcagcg
     5461 taggccgctg ggatcgtgag tgcgccgaat atcagcgtga ggggcaatag ggcgtggcgg
     5521 gccatccgag tctcctgggc ggcatgctcg cgcaaccgct cgggggccgc aagcgctcat
     5581 ccgtggggca acgtcacttg ctggccgtga aacctcctgc cctgggtcgc gggacggtgt
     5641 tcggcccccg gctccgggtg gctgaaatcc gcggcgccgg tcgagcgttg gccggtccat
     5701 gcatcgccgc acctgcctgt cgtggctcac cggagctgga gccctcggtc ttggaggtgc
     5761 gtggccgggg cgcgcagggg cagcggggac ggccgatgcc gcgacccgca acgcgacggt
     5821 gctcgctagc ctcggcgagc ccgggccgcc gctgcgtcgg tggcagtgga tcgtcgtgca
     5881 tcacaccgcg gccgagcacg cgacgctgcc tggcatcgac cgctatcacc gcaaccattt
     5941 cggcgatccg ctcggggccg agtaccactt catcatcaac aacggcaaga aggaccgcgc
     6001 caaccggccg atcgggctga tcgatgtcgc ccggtggaag tatcaggagc ccgcgtggca
     6061 cctgttcaag cccgacaacg cgccggactc gatcgccatc tgcctggtcg gcaacttcga
     6121 gaagcgggcg ttgcccgcgg agatgctggc gtcgctgacc gagctgacgc gcgcgctgat
     6181 gcagctgtgc gagatcccag cggagcgggt cagcacgcac cgcgtcgtcg acgagaagat
     6241 gacccagtgc cccggcaagc tgttcccccg ggaggcgttt ttaaaggggc tgtaggtcag
     6301 tcggcgagga tcgccagcac ggccggcagg tcgcgcgcgc tcaggccgta ttgtttgctg
     6361 gtgcgcacga gcttgtcggg gaaggccggc atgtcgtagt cgtcgtcgag cacgacgaag
     6421 cgggccggcg gggtcgcctg gcgctcgagc cacgcggcga cctcgagcac acgctggcgg
     6481 ccgtcgatgt cgggcgtgat gtcgacgatc tccgcgacgc aacctgcctc ggcgaagctg
     6541 gccttgaggc ccgcgaacga ggtcgagagg cgccacgtcg agctgacgac gaccaccgcg
     6601 ccggtcgcct cgacgatcgc gtttagcacc gagacgttgc gcgggtcgag gtagtcggcc
     6661 agcccacgcg agtgctgcgg ccgggtgttg atgctgttca tcacgccgtc gatgtcgagg
     6721 aagatgacct tgcgcggcac cgccacagca tacccgcgat cgacctcagc ggtcgctcac
     6781 gcggcgcaga aacgccggca ggccgagctc ctcgccgagt gtttcgtcct cctgcgcgag
     6841 gaacctggcc agcgtcgagc cggtcccgat cggcggccac gggggtggcg gctcgcggaa
     6901 cgccccgtcg ggcccgcgca tgtgcgagcg gtcggacgcg aaggtcgccc gcagcagctc
     6961 ggccgggacc acgcgaaacc gcggccgcag ctccggcggc aacacgacct cggggcgaaa
     7021 gctccggcgc aggtactgca ccgccggcac cgggcagccg agcgtggtat cgaggtccca
     7081 gatctcccac ggtcgctgcg cgagcacgac cacgtgataa tcccagaaca ccggctcacc
     7141 gggcgcctcg gcggcgcgct ggtgccagat cacgcaggcg tcgccgcggc cgcggatgaa
     7201 caccgccgcc gggtcgcgct gcgcgaggtc cgggtgcgta cacaggtggt acgcgttctc
     7261 ctcgcagtag aacggatggt agagatggtc gctcaaagcc cggtcttggc ctcgatcagg
     7321 ttcatgatgt cacagttgac ggcggcgatg cgctcgtcga tctcgggcca gcgctcgccc
     7381 gccttgtggc cgctcttggg cacctcgcag cggaacaggc ccatgttgat ctggtgggcg
     7441 tcgtccgtct cgagaaaacg gtccgaccac gcgtgccagt tggcgccgat gatcgccttg
     7501 aagccataaa actgctcgag ctgcgattga tagtaggcgc cgcgctggat ctgatcgccg
     7561 acgccgtcgt tggtcggcac gaacgagccc gcgccgccct tgttcgtcca gccgtcgatc
     7621 cgcgcgcgca cgctgaactc gctgatgatg atcggcaggc ccgacttctc ctcgatcagg
     7681 tgcaccccgt cggtgaacca cggtttttca aattcggcgt cgccagtata aatattgaag
     7741 gcgacgaggt cgaagccgct cggaccattc cgccgcagca ggtcgagcgg gttgtacttg
     7801 acctccgccg tgtcggtcgg caccggcgtg ctgccgtcct cgacccacac gtccgcgccg
     7861 ggctcgctcg ccggggtcca gaagcgaaag tgcgtgctcg cgccgatcgc catgcgcggc
     7921 gcgctgatga tgtggttcgg gtccgccgcg cgcacgcggg tggtgatcac ctcgacccac
     7981 gtctttaata aatggtcgtg gacgaagacc tggaggtcga cgcggcactg ttgattacag
     8041 tcgctgacgt aggggatcgg cctcgggccg acgtcgacga tcgtcgagaa gtccgcgccg
     8101 gggtagctgc tctcccaagc cttgttgagc gccggcaggt cggcgtagcg ctcgcgcagg
     8161 aagccggcga gcacgtggcg ggcacacttc ggggcgtcga tcgtcgagtc cgcggcgcag
     8221 tccgaccaca ggtatcgccg atagtcgcgg acgcccggcg tcttggcgcc cttgtcgaac
     8281 acgccgatct cgttgccgag gtaccagatc tgcaagttcg ggtcattctt gcgttcagcg
     8341 atctggccct gcgcgagctc gtcgatgtcg gcgaggaaca gcgggttgaa cgggtcgccg
     8401 aagcgggccg aggcgacgcc gctctcgagc accaccccct tggcgtcgcg cagcgcccgg
     8461 tcgtcgccgc cggggccgat gttgacgacg acgctgtacg gcgcctccgc gccgccctcc
     8521 gcgaacgggc ggatcatgta cgagtcgccc ggcgtgtcgt cgaagtcgtt gatgttgcta
     8581 aacgcgccga ccgagttgaa gcagtacggc tcctcgacgg tctcgtcgcc gctcttgccg
     8641 ttcgccagcc gggcccactc gatgcgcgcc gtccgggtcg gctcgaagcg cttgatccac
     8701 tggtcgatgc cgtcgcactc cttgccgcgc atcaccgtgt tgacgcccag catccacgtc
     8761 ttctcgccgg tcggggacac cagccaggtc cggccgtcgc ccaccggcgg ctcgatgtgc
     8821 caaaagctgc ccggcggctc gcccgtctcg gtcgtcgtca cgcccgtgtc cccggtcggc
     8881 tcggtcgtgg tcccggtcgt cccggtcggc tcggttccgc tcgtcgtgcc cgagtcgggc
//
LOCUS       DASWNJ010000919         2248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_16970, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000919 DASWNJ010000000
VERSION     DASWNJ010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16970"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VGB85_15580"
     CDS             <1..200
                     /locus_tag="VGB85_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135505.1"
                     /translation="VTPAAAATPAAADAKGEMKCAEGKCGEGACGGKKTDDGKAVPVD
                     AAKDAKAGDAVKPAEKPADKT"
     gene            complement(376..1290)
                     /locus_tag="VGB85_15585"
     CDS             complement(376..1290)
                     /locus_tag="VGB85_15585"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEY0135506.1"
                     /translation="MSARELIALGTASQVPTRHRNHNGYLLRWDDEGFLFDPGEGTQR
                     QMIYANVTTAAITRICITHFHGDHCLGLAGISQRISLDRVPHVVHIYYPGSGQKYYDR
                     LRHASIFHDVARVEAHPISGPGEILRDNKLSLSTLELDHGVEAWGYRLQEHDSRTMLP
                     DRLAEAGVRGPQVKKLIQDGEITIDGRTVLLDEVSSFRPGQSVAFVMDTRVCDNAVEL
                     ARGVDLLICESTYLSCDADRARDNGHLTAVEAAQIAAAAGARKLVLTHFSQRYNSVEP
                     FVTEAREFFPEVVAARDGDTISVPPRRV"
     gene            1427..2218
                     /locus_tag="VGB85_15590"
     CDS             1427..2218
                     /locus_tag="VGB85_15590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0135507.1"
                     /translation="MDDRDATATLQVHGSGAHVLVVEDNLVSQRVVVAMLAKLGHAVE
                     IANHGREALACVQRRRPGLVLMDCQMPVMDGFAATKAIRDGEQDGGSRLTIVAMTAFA
                     MDGDRERCIAAGMDDYIAKPMAIDTLAALLARWLGGDVIAAPVVAADDGPVSLALLRE
                     LEDELGPEELGELLAVLRRQAPAALAEMRGRLTAGDLQAVARLAHGLCGAAGNLGAQR
                     LSRELRAVEEACRTPGAAAPNDRTIAALERSFAEADAFFRAQLGL"
BASE COUNT          340 a          709 c          884 g          315 t
ORIGIN      
        1 aggtcacgcc cgcggccgcc gcgacgcccg cggccgccga cgccaagggc gagatgaagt
       61 gcgccgaggg caagtgcggc gagggtgcct gcggcggcaa gaagaccgac gacggcaagg
      121 cggtcccggt cgacgcggcc aaggacgcga aggccggcga cgcggtcaag ccggcggaga
      181 agccggccga caagacctga gctgtcccct gggacagggc acgcgcgagc cgcccgacga
      241 gaccgagctg tcctgcgggg caggtcccgc gaggcggtcg agtcggcgga ggagccggcc
      301 gacgagggcc aagctgtcct gcgggacagg tccgagaggc ggttgaggcc tgagcggtct
      361 ggccccctga accggtcaca cgcgacgcgg cggcacggag atcgtgtcgc cgtcgcgggc
      421 ggcgacgacc tccggaaaaa actcgcgggc ctcggtgacg aagggctcga ccgagttgta
      481 gcgctgagaa aagtgggtga gcacgagctt gcgcgcgccg gccgcggcgg cgatctgggc
      541 ggcctcgacg gcggtgaggt ggccgttgtc gcgggccctg tcggcgtcgc aggacaggta
      601 ggtcgactcg cagatcagca ggtcgacgcc gcgggcgagc tcgacggcgt tgtcacacac
      661 gcgggtgtcc atgacgaagg cgacgctctg gcccgggcga aagctgctga cctcgtcgag
      721 cagcacggtg cgaccgtcga tcgtgatctc gccgtcctgg atcagctttt ttacctgcgg
      781 gccgcgcacg ccggcctcgg cgaggcgatc gggcagcatc gtccggctgt cgtgctcctg
      841 caggcgatag ccccaggcct cgacgccgtg gtcgagctcg agggtcgaca ggctcagctt
      901 gttatcccgt aaaatttcgc cggggccgga gatcgggtgg gcctcgacgc gcgcgacgtc
      961 gtggaagatc gaggcgtggc gcaggcggtc gtagtacttc tggcccgagc cgggatagta
     1021 gatgtggacg acgtgcggga cgcggtcgag ggagatccgc tgcgagatgc cggcgagacc
     1081 gaggcagtga tcgccatgaa agtgcgtgat gcagatccgc gtgatcgcgg ccgtggtcac
     1141 gttggcgtag atcatctgtc gctgcgtgcc ctcacccggg tcgaagagga aaccttcgtc
     1201 gtcccagcgc agcagataac cattgtggtt gcggtggcgg gtcggcacct gactggcagt
     1261 gccgagggcg atcagttcgc gggcagacat ggctgggcgc gcgggagcat accctgaccc
     1321 gaggggcagg tgcgcgcccg gaagcgacgc gcgccacgcc ggcccccggc ggaccttgac
     1381 ccggtcctgg acggcgcgcg cggggtgata tggtggcagc gcagggatgg acgaccggga
     1441 cgcgaccgcc acgttgcagg tgcatggctc cggtgcccac gtgctggtcg tcgaggacaa
     1501 cctggtcagc cagcgtgtgg tggtcgcgat gctggcgaag ctgggccacg cggtcgagat
     1561 cgccaaccac ggccgcgagg cgctcgcatg cgtgcagcgc cggcgcccgg gcctggtatt
     1621 gatggactgc cagatgccgg tgatggacgg cttcgccgcg acgaaagcga tccgcgacgg
     1681 cgagcaggac ggcgggtcgc gcctgacgat cgtggcgatg acggccttcg cgatggacgg
     1741 cgaccgcgag cgctgcatcg ccgccggcat ggacgactac atcgccaagc cgatggcgat
     1801 cgacacgctc gcggcgttgc tcgcccgctg gctcggcgga gacgtcatcg ctgcgccggt
     1861 ggtcgccgcc gatgatggcc cggtttcgct ggccctgctg cgcgagctgg aggacgagct
     1921 cgggcccgag gagctcggcg agctgctcgc tgtgctgcgc cgccaggcgc ccgcggcgct
     1981 ggccgagatg cggggccgtt tgaccgcagg tgacctgcag gcggtcgctc ggctcgcaca
     2041 cggcttatgc ggcgcggcgg ggaatctcgg ggcgcagcgg ctgtctcgcg agttgcgggc
     2101 ggtcgaggag gcctgtcgaa cgccgggggc tgcggccccg aacgatcgaa cgatcgctgc
     2161 gctcgagcgc agcttcgccg aggccgacgc gttctttcgc gcgcagctgg gcctgtgatg
     2221 ccgctcggga cgaattggca gttcgagc
//
LOCUS       DASWNJ010000920         2832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_122053, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000920 DASWNJ010000000
VERSION     DASWNJ010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122053"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VGB85_15595"
     CDS             <1..550
                     /locus_tag="VGB85_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135508.1"
                     /translation="LVYQLEHGLGSCLRARAEMQVRSLSADDRKICGQLGISLGALVV
                     FVPALLDATAVLRRAALWSAAYGRPPALPPAGKVSVPVDSNVEPSWYHAIGYPPFGPR
                     AVRADQAERAFSRLLQATSSGPVALPQELASWLGCSQGEAGEVAEAMGLTRVDDERYA
                     FVRPERGRHPHQSRSRRRGGGR"
     gene            complement(555..2240)
                     /locus_tag="VGB85_15600"
     CDS             complement(555..2240)
                     /locus_tag="VGB85_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135509.1"
                     /translation="MSNRTPALGLLLLAACPVPMNIGDPIEGPATTTGQTTTSNPGVS
                     SSPTTTGATDDLVSTSQGVNETEASSTTTADTTSSGTTGTGTDTDTSTGEPPAPECTP
                     TGDALESAECLAIDPDRPFCQEGACVGCGALAADACTTASAGERPICLPAGACVQCDS
                     NDAVMQGQCEADQPHCNLETHTCEGCFEHSECPQTACEIAVRKCFPANQKIYVRQGPT
                     PGSPCSTKPGMGGTMELPYCDFEIATLGVQQNGFSSGYTFHLMSNDDAPGDPGAVVIT
                     GGDAHVAYAFTHDFGGLVDKHTRFKGIGPMILVPNNVTLYLVNWGILIDKPQGDSSRG
                     IDCQAGGSVWLDDSRVLFARGPGIRGTGCDIHLRRTSVALGETEGIDMTGGSLHAVNS
                     FIAGNINIVGKNGGGLHLRDGADVDIVYSTIADNSNEPSQVPGQFGDSIHCDNAATIK
                     IRNSIIFRKPANNNFSVVCPEGQVDIDWSVVDSEFNGNNNTKFAAEDLLEALMPEGLT
                     GSYRVASQMAGEQFFATAARWQPGDPQGDVDREARNAVPDGEDYAGADVFNSP"
     gene            complement(2287..>2832)
                     /locus_tag="VGB85_15605"
     CDS             complement(2287..>2832)
                     /locus_tag="VGB85_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135510.1"
                     /translation="AAVIAALAVGASLGEHEARERDPAAAFHGAARTTGVVAPRPETR
                     ASTAPPPPVVIEHTAVPLPDDPAPQIAPEPPPAPTREPRARREPAPAPARTLADEMRR
                     MRPAQRALAAGDPVTALAELERYALEFPDGHLREEYLAMHAIARCDAGHAEGRREADE
                     FLRARPLSIFAGRVRSACATP"
BASE COUNT          321 a          935 c         1121 g          455 t
ORIGIN      
        1 cctcgtgtat cagctcgagc acgggctcgg ctcgtgcctg cgcgcgcgcg cggagatgca
       61 ggtgcgctcg ctgtcggcgg acgaccggaa gatctgcggg cagctcggca tctcgctcgg
      121 ggcgctcgtc gtgttcgtgc cggcgctgct cgacgcgacc gcggtgctgc gccgcgcggc
      181 gctgtggtcc gcggcgtacg ggcgaccgcc cgcgctgccg ccggccggca aggtgtcggt
      241 gccggtcgac agcaacgtcg aaccctcgtg gtaccacgcg atcggctacc cgccgttcgg
      301 gccgcgcgcg gtgcgggccg accaggccga gcgggccttt agtcgcctgc tgcaagcgac
      361 cagctccggg ccggtcgcgc tgccgcagga gctcgcctcg tggctcggct gctcgcaggg
      421 cgaggccggc gaggtcgccg aggcgatggg gctgacgcgc gtcgacgacg agcgctacgc
      481 gttcgtgcga cccgagcgcg gccggcatcc gcaccagtcg cgctcgcgcc ggcgcggcgg
      541 cggccgctag cacctcaggg gctgttgaac acgtcggcgc cggcgtagtc ctcgccgtcg
      601 ggcacggcgt tgcgggcctc gcggtcgacg tcgccctggg ggtcaccggg ctgccacctc
      661 gcggcggtcg caaagaattg ctcgccggcc atctgggagg cgacgcggta gctaccggtc
      721 aggccctcgg gcatgagggc ctcgaggagg tcctcggcgg cgaacttggt gttgttgttg
      781 ccgttgaatt cgctgtcgac gaccgaccag tcgatgtcca cctgaccctc gggacagacg
      841 acgctgaagt tgttgttggc cggcttgcgg aagatgatcg agttgcggat cttgatggtg
      901 gccgcgttgt cgcagtggat cgagtcgccg aactgaccgg gcacctgcga cggctcgttg
      961 ctgttgtcgg cgatcgtgct gtagacgatg tcgacgtcgg cgccgtcgcg caggtgcagg
     1021 ccgccgccgt tcttgccgac gatgttgatg ttgccggcga tgaagctgtt gaccgcgtgc
     1081 agcgagccgc cggtcatgtc gatgccctcg gtctcgccca gggcgacgct ggtgcggcgc
     1141 aggtggatgt cgcagccggt gccgcggatg cccgggccgc gggcgaacag gacccgcgag
     1201 tcgtcgagcc acaccgagcc gcccgcctgg cagtcgatgc cgcgcgagga gtcgccctgc
     1261 ggcttgtcga tcaggatccc ccagttcacg aggtacagcg tgacgttgtt gggcacgagg
     1321 atcatcggtc cgatgccctt gaagcgggtg tgcttgtcga ccaggccgcc gaagtcgtgg
     1381 gtgaaggcgt acgcgacgtg ggcgtcgccg ccggtgatga cgaccgcgcc cggatcaccg
     1441 ggcgcgtcgt cgttgctcat caggtggaac gtgtagccgg acgagaagcc gttttgctgc
     1501 acgccgaggg tcgcgatctc gaagtcgcag tacgggagct ccatcgtgcc gcccatgccc
     1561 ggcttggtcg agcacgggct gccgggggtc gggccctggc gcacgtagat cttctggttg
     1621 gccgggaagc acttgcgcac cgcgatctcg caggcggtct gcgggcactc gctgtgctcg
     1681 aagcagccct cgcaggtgtg ggtctcgagg ttgcagtgcg gctggtcggc ctcgcactga
     1741 ccctgcatga ccgcgtcgtt gctgtcgcac tgcacgcagg cgcccgcggg caggcagatc
     1801 ggtcgctcgc cggcgctcgc ggtggtgcac gcgtcggccg cgagcgcgcc gcagccgacg
     1861 cacgcgccct cctggcaaaa cgggcggtcg gggtcgatcg ccaggcactc ggcgctctcg
     1921 agcgcgtcgc cggtcggcgt gcactcgggc gcgggcggct cgccggtgct cgtgtcggtg
     1981 tccgtgccag tgccggtggt gccgctgctc gtggtgtcgg ccgtggtggt gctgctggcc
     2041 tcggtctcgt tgacgccctg cgaggtcgag accagatcgt cggtggcgcc ggtcgtggtc
     2101 gggctcgagc tgacgccggg gttgctcgtg gtggtctggc cggtggtcgt cgcggggcct
     2161 tcgatcgggt cgccgatgtt catcgggact ggacaggcgg cgagcaggag gaggccgagg
     2221 gcgggcgttc ggttggacat catccgtgga tgtccgcgat cgacgagctt cgttgccgct
     2281 cggcgatcat ggcgtcgcgc aggcgctgcg gacccgcccg gcgaagatcg acagcggccg
     2341 cgcgcgcagg aactcgtcgg cctcgcgccg gccctcggcg tggccggcgt cgcagcgcgc
     2401 gatcgcgtgc atcgcgaggt actcctcgcg caggtggccg tccgggaact cgagcgcgta
     2461 gcgctcgagc tcggcgagcg cggtgaccgg atcgccggcc gcgagcgcgc gctgggccgg
     2521 acgcatgcgt cgcatctcgt cggcgagcgt gcgcgccggg gcaggcgcgg gctcgcggcg
     2581 ggcccgcggc tctcgcgtcg gcgccggcgg tggttcgggt gcgatctgcg gggccggatc
     2641 gtcgggcagc gggacagcgg tgtgctcgat gacgacgggt ggcggcgggg ccgtcgaggc
     2701 gcgcgtctcg gggcggggcg ccaccacgcc ggtggtcctg gcggcgccgt gaaacgccgc
     2761 ggcggggtca cgctcgcggg cctcgtgctc gccgagcgaa gcgccgaccg cgagcgcggc
     2821 gatcaccgcg gc
//
LOCUS       DASWNJ010000921         5322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_300242, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000921 DASWNJ010000000
VERSION     DASWNJ010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300242"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VGB85_15610"
     CDS             complement(<1..330)
                     /locus_tag="VGB85_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135511.1"
                     /translation="MAHPDDPDDFDLTVLEDTRETFAEHLGDYASLDDYFRGQLEELL
                     VPGGLWLLECLDMHEVRQRFEGGRYRYFVARGGLPDRGVTAPPDAVRCAHGSPAAGSS
                     MRLSASTT"
     gene            418..690
                     /locus_tag="VGB85_15615"
     CDS             418..690
                     /locus_tag="VGB85_15615"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY0135512.1"
                     /translation="MSSRRDWVTTAQVVEAAGVHGSTLGRWRRRGLLPEPIFVSLGKR
                     GRTQRWPPHAPAQAAWVKARLEDGWTFDEITAALERGEFKPSSTGL"
     gene            complement(687..1163)
                     /locus_tag="VGB85_15620"
     CDS             complement(687..1163)
                     /locus_tag="VGB85_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135513.1"
                     /translation="MIQASPEARGSAAKFVLDNFVGFVKSWRAQPDDNVGALVVIDGD
                     EHGLQRRRQQLTKRLLEAGELKLDPADPRFVILVPCWHIETWIAWLCGHRPVDEHTCY
                     KPEDPQGGEVGRKIRSGEYSARAAARSWTPRHSEEVTFVPSLAAARDELRRLGVAI"
     gene            complement(1261..2391)
                     /locus_tag="VGB85_15625"
     CDS             complement(1261..2391)
                     /locus_tag="VGB85_15625"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0135514.1"
                     /translation="MLTRVAIRNYNCFADVELELPRRLLLVGSNGSGKTSLWEALVGV
                     QDVIVRGTDVADVFPTRSLTRWLRHEGVQRFTIDVRSGVDTYTYSLELQHDTTRLTAS
                     IHEERLTLAGQVLYENRGGEVRIFGDVPRADGQPRTAFPFGRKRSFLPDIEARQDNTH
                     IMAFRAAVGDFWLIKHAASQISATTAEEGGWLERDGSNFASWFRGVLVDRPEVGSQLN
                     DALRPALPGLQKIAFEKVSQKVRELMLHFRTEDHDYSLSAGELSDGQRSLVLLHGLLV
                     GAFDHSGLAFLDEPEIGLAPHEMQPWLAQASAALEEHDGQMIVISHHPAVIDYLAPER
                     TLRFSRPGGGPTRIDEVTLETTGGTRVSEWLSIPWVYEDEHE"
     gene            complement(2478..3032)
                     /gene="frr"
                     /locus_tag="VGB85_15630"
     CDS             complement(2478..3032)
                     /gene="frr"
                     /locus_tag="VGB85_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEY0135515.1"
                     /translation="MDEALELAKEGMEEAIDRLRKSLTKVRAGRANPAMLDGVRVDNY
                     GSMMPLNQVATVSVGDARLLVIKPWDRNMIGPVEKAINAQNLGLNPQSDGVVIRLAIP
                     PLTEERRKGLVKQVKEITEEAKVAIRESRREVNEMLKEAEKKRALSEDDLKKGLEKVQ
                     DVHDRYIKQVNEITAKKEAEIMEV"
     gene            complement(3052..3801)
                     /gene="pyrH"
                     /locus_tag="VGB85_15635"
     CDS             complement(3052..3801)
                     /gene="pyrH"
                     /locus_tag="VGB85_15635"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEY0135516.1"
                     /translation="MEARPRYKRILLKLSGEALQGGQGYGIDAEVLRTIAGEIEDLIG
                     LGVEVAVVIGGGNIFRGLSAAASGMDRASADYMGMLATVMNSLALQDALEQRGVHTRV
                     LSAIEMREVAETYVRRRAERHLEKGRVVIFGAGTGNPYFTTDTAAALRAMEIHAEVLM
                     KATKVDGVYDKDPHKHADAVRYSRLTYLDVLSQGLDVMDSTAIALCKENNLPILVFNM
                     LERNNIRRVVCGEELGTLVAPDGESVLAGTP"
     gene            complement(3889..4554)
                     /gene="tsf"
                     /locus_tag="VGB85_15640"
     CDS             complement(3889..4554)
                     /gene="tsf"
                     /locus_tag="VGB85_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEY0135517.1"
                     /translation="MPVTVTPAMIKELRERTAAGLMDCKKALTETDGEMEKAIEFLRK
                     KGLAAAAKKSGRLASEGVVTSYVHSNNRVGVLLEVNCETDFVAQTPDFKQFCADVSMQ
                     IAAMNPVAVRREEMDTSKVEAERGLLREKALAEGKPEKMVDKIVDGQIGKYYSENVLL
                     EQKFVKDDKKTIEQIQTEIVAKLGENIKIRRFTRYELGEGLEKKNENFADEVAAQIAA
                     AKA"
     gene            complement(4558..>5322)
                     /gene="rpsB"
                     /locus_tag="VGB85_15645"
     CDS             complement(4558..>5322)
                     /gene="rpsB"
                     /locus_tag="VGB85_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY0135518.1"
                     /translation="RNGIHIIDLQQTAGLFRRAYAFIRQCAAEGKPVLFVGTKKQASD
                     VMALEATRAGQYYVVSRWLGGTLTNWTTVRQSVDKLRGIERMSQDGTYEQITKKEALM
                     LERKRGKLERNLGGIKDMPKLPGAVFVIDPVKEHIAVVEANRLGVPVVAVADTNADPR
                     LIQYVIPGNDDAIRAIKLFCSRMADACIEGAKLGKSRAVNDPHEGRDPEPIRVTTGGD
                     GPRVEIVSRRTQLPAPEAAATPEEDATTTFSDSSQE"
BASE COUNT          759 a         1801 c         1782 g          980 t
ORIGIN      
        1 cgtcgtcgac gcgctcaagc gcatcgagct gcccgcagcc ggcgagccgt gagcgcagcg
       61 aacggcgtcc ggcggcgccg tcacgccccg gtccggtaga ccgccccgcg cgacgaaata
      121 ccggtagcgc ccgccctcga agcgctgcct gacctcgtgc atgtcgagac actcgagcaa
      181 ccacaggccg cccggcacga gcagctcctc gagctggccc cggaagtagt cgtcgagtga
      241 ggcgtagtcc ccgaggtgct ccgcgaacgt ctcgcgggtg tcctcgagca cggtgagatc
      301 gaagtcgtcg gggtcgtcgg gatgggccat ggcctcgtac gtctaccgcc actttgcttt
      361 actttgcatt aaattgcaaa acctcaccat ataagacgca cgcttagact ccgcagcgtg
      421 agcagccgcc gagactgggt gaccaccgcg caggtggtcg aggccgccgg ggtgcacggg
      481 agcactctgg gccgctggcg ccgacgcggc ctgttacccg agccgatctt cgtctcgctc
      541 ggcaagcgcg gccgaaccca gcgctggccg ccacacgcac cggcccaggc cgcgtgggtg
      601 aaggcccgcc tcgaggacgg ctggaccttc gacgagatca ccgccgcgct cgagcgcggc
      661 gagttcaagc catcctcgac tggcctctaa atggcgacgc cgagccgtcg cagctcatcc
      721 cgggcggccg cgagcgaagg gacgaacgtc acctcctcgc tgtgccgcgg agtccacgat
      781 ctagctgccg cgcgcgccga gtactcgccg ctcctgatct tgcggccgac ctcgccccct
      841 tgcgggtcct ccggcttgta gcaggtgtgc tcatcgaccg gccgatggcc acacagccag
      901 gcgatccagg tctcgatgtg ccagcacggg acgaggatga caaaccgtgg atccgcggga
      961 tccagcttca attcgccggc ttcgagcagc ctcttggtga gctgttgtcg acgacgctgg
     1021 aggccgtgct cgtccccgtc gatcacgacg agcgcgccga cgttgtcatc gggctgggcg
     1081 cgccatgact tcacgaagcc gacgaaatta tcgagcacga acttcgccgc cgaaccgcgc
     1141 gcctcgggtg aggcctggat cattcgctgt cgcggtccca cgtcccatcg atccgccagg
     1201 cgctcgatga agccgcggtg gagcttatcc tcgcagagga cacgcagccg gagcctctgc
     1261 ctattcatgc tcgtcctcgt aaacccacgg gatcgacaac cactccgaga cgcgggtacc
     1321 gcccgtcgtc tccagcgtga cctcgtcgat ccgcgtcggc ccgccgccgg gacgagagaa
     1381 ccgcagcgtg cgctccggcg cgagatagtc gatcaccgcc ggatgatgcg agatcacgat
     1441 catctggcca tcgtgctcct cgagcgccgc ggacgcctgc gcgagccagg gctgcatctc
     1501 gtggggcgcc aggccgatct cgggctcgtc caggaatgcg aggccactgt ggtcgaaggc
     1561 gccgacgagc aggccgtgca gcagcacgag gctgcgttga ccgtcggaca gctcgcccgc
     1621 ggacagcgag tagtcgtggt cctccgtgcg aaagtgcagc atcagctcgc ggaccttctg
     1681 gctgaccttt tcaaaggcga ttttctgcaa gcccggcagc gccggtcgca atgcgtcgtt
     1741 gagctgactg ccgacctccg gccggtcgac gagcacgccg cgaaaccatg acgcgaagtt
     1801 gctgccgtct cgttcgagcc agccgccctc ctccgccgtc gtcgccgaga tctgtgacgc
     1861 cgcgtgcttg atcagccaga agtcgccgac cgcggcgcga aacgccatga tgtgggtgtt
     1921 gtcctggcgc gcctcgatat cgggcaagaa cgatcgcttg cgcccgaagg ggaaggccgt
     1981 gcgtggctgt ccgtccgcgc gcggcacgtc gccgaagatc cggacctccc cgccgcggtt
     2041 ctcgtagagg acctgccccg cgagcgtgag ccgctcctcg tggattgacg cggtgaggcg
     2101 ggtcgtatcg tgctgcagct cgagcgagta cgtgtacgtg tcgacgcccg accgcacgtc
     2161 gatggtgaag cgctgaacgc cctcgtgccg cagccagcgg gtcagagatc gcgtcgggaa
     2221 cacgtccgcc acgtcggtcc cgcggacgat cacgtcctgc actccgacca gcgcctccca
     2281 caggctcgtc ttgcccgagc cgttgctgcc gacgagcagc agcctccgcg gcagctccag
     2341 ctcgacgtcg gcgaagcagt tgtagttccg gatcgctact ctggtgagca tgctggctcc
     2401 tcgccgtgaa acgcaaacag cccaggcggc gcggagccgt gggctgttgg tcggcggcgc
     2461 gcccaggggg ctcgcgctca gacctccatg atctccgctt ccttcttcgc ggtgatctcg
     2521 ttgacctgtt taatatagcg gtcgtggacg tcctggactt tttccaggcc ctttttaagg
     2581 tcgtcctcgg acagggcgcg cttcttctcg gcctccttga gcatctcgtt gacctcgcgg
     2641 cgggactcac ggatggcgac cttggcctcc tccgtgatct ccttgacctg cttgacgagg
     2701 cctttgcggc gctcctcggt cagcgggggg atggcgagtc ggatgacgac gccgtcgctc
     2761 tgcgggttga ggccgaggtt ttgggcgttg atcgccttct cgaccgggcc gatcatgttg
     2821 cggtcccagg gcttgatgac cagcaggcgg gcgtcgccca cgctgacggt tgcgacctgg
     2881 ttgagcggca tcatcgagcc gtagttgtcg acgcgcacgc cgtcgagcat ggccgggttg
     2941 gcccggccgg cgcggacctt ggtcaggctc ttgcgcagac ggtcgatcgc ctcctccatg
     3001 ccttcctttg ccagttcgag tgcctcgtcc atcgtcgttg ctccttcgat ctcagggtgt
     3061 gcccgcgagg acgctctcgc cgtccggggc gaccagtgta ccaagctcct cgccgcacac
     3121 cacccggcgg atgttgttcc gctcgagcat gttgaagacg aggatcggga ggttgttctc
     3181 tttgcagagg gcgatcgccg tgctgtccat gacgtcgagc ccctggctca gcacgtcgag
     3241 gtaggtcagc cggctgtagc ggaccgcgtc cgcgtgcttg tgcgggtcct tgtcgtagac
     3301 cccgtcgacc ttggtcgcct tcatcagcac ctcggcgtgg atctccatcg ctcgcagggc
     3361 ggcggccgtg tcggtggtga agtaggggtt gccggtgccg gccccgaaga tgaccacgcg
     3421 gcccttctcg aggtggcgtt cggcccggcg gcgcacgtag gtctcggcga cctcgcgcat
     3481 ctcgatcgcc gagagcacgc gggtgtgcac gccgcgctgc tcgagcgcgt cctgcagcgc
     3541 cagcgagttc atgacggtcg ccagcatgcc catgtagtcg gcgctggcgc ggtccatgcc
     3601 cgacgcagct gcgctcaggc cgcggaagat gttgccgccg ccgatgacga cggcgacctc
     3661 gaccccgagc ccgatcaggt cctcgatctc gccggcgatc gtgcgcagga cctcggcgtc
     3721 gatgccgtag ccctggccgc cctgcagggc ctcgccggac agttttaaca ggatcctctt
     3781 gtagcgcggg cgagcttcca tgcgtcttga accttccgcg tccgtccgcg aaccgaacgg
     3841 ctcgcgaaaa gggcgggccc gttggcgccc gccctctcat ctcatgactc aggccttggc
     3901 ggccgcgatc tgggcggcga cctcgtcggc gaagttctcg ttcttcttct cgaggccctc
     3961 accgagctcg tagcgggtga agcggcggat cttgatgttc tcgccgagct tcgccacgat
     4021 ctccgtctgg atctgctcga tcgtcttctt gtcgtccttg acgaactttt gctcgagcag
     4081 gacgttctcc gagtagtact tgccgatctg accgtcgacg atcttgtcga ccatcttctc
     4141 gggcttgccc tcggcgagcg ccttctcgcg cagcaggccg cgctcggcct cgaccttgct
     4201 cgtgtccatc tcctcgcggc ggaccgcgac ggggttcatc gccgcgatct gcatcgagac
     4261 gtcggcgcag aactgcttga agtcgggggt ctgcgcgacg aagtccgtct cgcagttgac
     4321 ctcgagcagc acgccgacgc ggttgttgct gtggacgtac gacgtgacga cgccctcgct
     4381 cgcgaggcga cccgacttct tggcggcggc ggcgaggccc ttcttgcgca ggaactcgat
     4441 cgccttctcc atctcaccgt cggtctcggt gagcgccttc ttgcagtcca tgaggcccgc
     4501 ggcggtgcgc tcgcggagct ccttgatcat tgcgggggtg acggtgacgg gcatggtcta
     4561 ctcctgcgag ctgtcggaga aggtggtcgt cgcgtcctct tcgggggtcg cggcggcctc
     4621 gggggcgggc agctgggtgc ggcgcgagac gatctcgacg cgcggaccgt cgccgccggt
     4681 ggtgacgcgg atcggctcgg ggtcacggcc ctcgtgcggg tcgttgacgg cgcggctctt
     4741 gccgagcttg gcgccctcga tgcaggcgtc ggccatgcgc gagcagaaca gcttgatcgc
     4801 acggatcgcg tcgtcgttgc ccgggatgac gtactggatc aggcgcgggt cggcgttggt
     4861 gtcggcgacg gcgacgaccg ggacgccgag gcggttggcc tcgaccacgg cgatgtgctc
     4921 cttcaccggg tcgatgacga acacggcgcc cggcagcttc ggcatgtcct tgatgccgcc
     4981 gaggttgcgc tcgagcttgc cgcgcttgcg ctcgagcatc agcgcctcct tcttggtgat
     5041 ctgctcgtag gtgccgtcct ggctcatgcg ctcgatgccg cgcagcttgt cgaccgactg
     5101 acggaccgtg gtccagttgg tcagggtgcc gccgagccag cggctgacga cgtagtactg
     5161 accggcgcgc gtggcctcga gggccatcac gtccgaagcc tgcttcttgg tgccgacgaa
     5221 cagcaccggc ttgccctcgg ccgcgcactg gcggatgaac gcgtaggcgc ggcggaacag
     5281 accggcggtc tgctggaggt cgatgatgtg gatgccgttg cg
//
LOCUS       DASWNJ010000922         3938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_582285, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000922 DASWNJ010000000
VERSION     DASWNJ010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582285"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VGB85_15650"
     CDS             <1..528
                     /locus_tag="VGB85_15650"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEY0135519.1"
                     /translation="ASGRITVRSIDPAELAPGEFAHAVRQAVEEGGARLIVIDSLNGY
                     LHAMPDEQFITLQRLELLTYLRQRGVVTVMIAAQRGLMGPSMSTPIDVSYLADGVVLF
                     RFFESEGRIRRAMSVLKKRSGAHEDTIRELVFSSQGIAVGEPLHAFHGVLTGVPTFVG
                     ESAPPMDPPDGRRAE"
     gene            525..1694
                     /locus_tag="VGB85_15655"
     CDS             525..1694
                     /locus_tag="VGB85_15655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0135520.1"
                     /translation="MTHERSPDGRFLIAAPTGRDAKLACSVLLRADIDALACGGIDEL
                     CREAGRGCAGLLLAEEVLVPTTVQRLKELVAAQEPWSDLPMLLFTGASATLPTRGPTA
                     ALLRQLGNVTLLERPLRPITMVSAVQAALRARQRQYEARTMMASMAEGLRQRDQFLAM
                     LGHELRNPLGAILFGIDVLEHGGPERATKCYATIRRQTRHLARLVDDLLDVSRVTSGK
                     IVLKRGPVDLAELLRRCVETLEPRAQEQHQRLDLDVDGEPAVLAGDEVRLEQIFTNLI
                     GNALKYTPASGHIAVRLRRVDAEAIVSVTDDGSGIAADTLPHVFDLFTQAKPTLDRAQ
                     GGLGIGLTLVRTLVQLHGGSVEAASEGLGRGSEFTVRLPLVVQSESVVQPSPNRP"
     gene            complement(1837..2934)
                     /locus_tag="VGB85_15660"
     CDS             complement(1837..2934)
                     /locus_tag="VGB85_15660"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0135521.1"
                     /translation="MRRWVVGWVLLNGGCLDSNPVFIDPMTSSESGETTSAPPATDSA
                     ATTDAPGTSSPGTTTYTTSSTSTTTGPTSETDSPDTEGSIGPSTDSNSTIVPMPETKC
                     GDGILDPDEECDDGNSSPNDDCTDLCKMAVCGDNEVYYGVEECDDGVNGVPTDGCFDD
                     CTLPVCGDKFWDIYEECEDGNIEDDDGCEHDCKLTQKIIFVSSKLFTGDMKGLAGADN
                     ACNQMAMDAQNGPLPGTYLAWLSTNEESPALRMKHSIYPYVRSDRSHIADNWEGLVTT
                     MKFTPINLDENGNKHPGIVPTPDSCGWSVVYTNTLASGKGAGLGKDCMGWTSTFGETL
                     AGSIGLGQVGWTDFCNVMKCHIKAPIYCVQQ"
     gene            3006..3398
                     /locus_tag="VGB85_15665"
     CDS             3006..3398
                     /locus_tag="VGB85_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135522.1"
                     /translation="MPLLPPRRSAVWLLVLLGSAALLAAGLLWADSAGPGTPAQDTVL
                     AIVGVSGLIAAVLTAFGAFGARATYACAMIGLLIGLARMIWAAEHPTHGMEDLAAIAN
                     FYLYGVIGLGVGALIDFGRWIGARQRRK"
     gene            complement(3455..>3938)
                     /locus_tag="VGB85_15670"
     CDS             complement(3455..>3938)
                     /locus_tag="VGB85_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135523.1"
                     /translation="AALEKKINDPKEKLNTVDVDGDGKIDKIQVVEIQKPNQAKVFEL
                     HVIPSKTKKKEDEVIIAFIHFEPDVETKKLVVKATYAPIVIGYDTIVYEYFVPIEVKG
                     ETIVVVDTHPFYGWLFLPARPVFVGVYVYSAPPPPVIIIEHGWHHKHKHKGKGKGKWH
                     "
BASE COUNT          561 a         1361 c         1321 g          695 t
ORIGIN      
        1 gcctcgggca ggatcaccgt gcgatcgatc gacccggccg agctcgcacc cggggagttc
       61 gcccacgcgg tgcgccaagc ggtcgaggag ggcggcgcgc ggctcatcgt catcgacagc
      121 ttgaacggct acctgcacgc gatgccggac gagcagttca tcaccttgca gcggctcgag
      181 ctgctgacct acctgcgcca gcgcggcgtc gtcacggtga tgatcgccgc gcagcgcggc
      241 ctgatggggc cgtcgatgtc gacgcccatc gatgtcagct acctcgcgga cggggtcgtg
      301 ctgtttcgct tcttcgagtc ggagggccgg atccgtcggg cgatgtcggt cctgaagaag
      361 cgatcgggag cgcacgagga cacgatccgc gagctggtgt tctcgtcgca ggggatcgcc
      421 gtcggtgaac cgctgcacgc gtttcacggg gtcttgaccg gcgtgccgac ctttgtcggc
      481 gagtcggcgc ctccgatgga tcctcctgac ggacggcgag ccgagtgacg catgagcgca
      541 gcccggacgg gcgcttcctg atcgccgctc ccactggccg cgacgccaag ctggcgtgct
      601 cggtgctgct gcgggcggac atcgacgcgc tcgcctgcgg tgggatcgac gagctgtgcc
      661 gggaggccgg gcgcggctgc gccggcctgt tgctcgcgga ggaggtgctg gtgcccacga
      721 ccgtgcagcg gctgaaggag ctggtggcgg cgcaggagcc ctggtccgac ctccccatgc
      781 tcctgttcac cggcgcaagc gccaccctgc cgactcgcgg tccaacggcc gcgctcctgc
      841 ggcagctcgg caacgtcacg ctgctcgagc gaccgctacg gccgatcacg atggtcagcg
      901 cggtgcaggc ggccctgcga gcgcgccagc ggcagtacga ggcgcgaacg atgatggcca
      961 gcatggccga ggggctgcgc cagcgcgacc agttcctggc catgctcggg cacgagctgc
     1021 gaaacccgct gggcgcgatc ctgttcggca tcgacgtgct cgagcatggc ggccccgagc
     1081 gggccacgaa gtgctacgcg accattcgca ggcagacccg acacctggcc cgtctggtcg
     1141 acgacctgct cgacgtctcg cgcgtgacct cggggaagat cgtcctcaag cgcggcccgg
     1201 tcgacctcgc ggagctgctc cggcgctgcg tcgaaaccct cgagccgcgc gcgcaggagc
     1261 agcaccagcg gctcgatctc gacgtagacg gcgagccggc cgtgctcgct ggtgacgagg
     1321 tccgcctcga gcagatcttc acgaacttga tcggcaacgc cctgaagtac acgcccgcca
     1381 gcggccacat cgcggtgcgc ctgcgccggg tcgacgccga ggcgatcgtg tccgtgaccg
     1441 acgacggctc gggcatcgcc gccgacacct tgccccacgt cttcgacctg ttcacgcagg
     1501 ccaagccgac cctcgaccgc gcccagggcg ggctcgggat cgggctcacg ctcgtgcgca
     1561 cgctcgtgca gttgcacggc ggctccgtcg aggccgcgag cgagggtctc ggtcgcggca
     1621 gcgaattcac cgtccgcctc ccgctcgtgg tccagtccga gtccgtggtg cagccgtcgc
     1681 cgaaccgccc ttgaagccgc acatgctctc gtccccggcc gctacatcga ccgtcgcggc
     1741 gccgggtccc gcgcgctcag cgcgctggag agctgatgga ggcgtgcgac cgcccgcctc
     1801 caagctctcg taatacgaga tcaattagcc ataactttac tgttgcacgc aatagatcgg
     1861 cgccttgatg tggcacttca tgacattgca gaagtcggtc cagccgacct ggccgaggcc
     1921 gatcgacccg gccagggtct cgccgaacgt gctggtccag cccatgcagt cctttccgag
     1981 cccggcgccc ttgccggacg cgagcgtgtt ggtatacacg accgaccagc cgcagctgtc
     2041 gggcgtcggc acgatgcccg ggtgcttgtt tccgttctcg tcgaggttga tcggcgtgaa
     2101 cttcatggtg gtcaccagac cctcccagtt atcggcgatg tgcgagcggt cgctgcggac
     2161 gtaggggtag atggagtgct tcatgcgaag cgccggggac tcctcattcg tgctcagcca
     2221 ggccaaatag gtgcccggca gcgggccgtt ctgcgcgtcc atcgccatct ggttgcaggc
     2281 attgtccgcc ccggcgaggc ccttcatgtc gccggtgaac agcttactgc tgacgaagat
     2341 gatcttctgg gtcagcttgc aatcgtgctc gcagccgtcg tcgtcctcga tgttcccgtc
     2401 ttcgcactcc tcgtagatgt cccaaaattt gtcgccgcag acgggcaagg tgcaatcgtc
     2461 gaagcaaccg tcggtgggga cgccgttgac gccgtcgtcg cactcctcca ccccgtagta
     2521 gacctcattg tcgccgcaca cggccatctt gcacaaatcg gtacagtcat cgttcgggct
     2581 ggaattaccg tcgtcacact cctcgtcggg gtcgaggatg ccgtcgccac acttcgtctc
     2641 gggcatcggc acgatcgtcg agttgctgtc ggtgctcggc ccgatcgagc cctccgtgtc
     2701 cgggctgtcc gtctccgagg tcggcccggt cgtcgtcgag gtcgacgacg tcgtgtacgt
     2761 cgtcgtgccg ggcgagctgg tgcccggggc gtccgtcgtc gccgccgagt cggtcgcggg
     2821 tggcgcactg gtcgtctccc ccgactcgct gctcgtcatc gggtcgatga agaccgggtt
     2881 cgagtcgaga catccgccgt tcaacagcac ccagccgacc acccaccgtc gcatgcccgc
     2941 caggatacca gcagtccggc gccggcgtgg ccgcaccggc cgcgcgctcc gctacactga
     3001 gcgccatgcc gctcctcccg ccccgtcgca gcgccgtctg gctcctcgtg ctcctggggt
     3061 ccgccgcgtt actggccgcc gggctgctgt gggcggactc ggccggcccc ggcacgccgg
     3121 cccaggacac cgtgctggcg atcgtcggcg tcagcggcct gatcgccgcc gtcctgaccg
     3181 cgttcggggc cttcggcgcg cgagcgacct acgcctgcgc gatgatcggc ctgctgatcg
     3241 gcctcgcgcg catgatctgg gccgccgagc acccgaccca cggcatggag gacctcgccg
     3301 cgatcgccaa cttctacctc tacggcgtga tcggcctcgg cgtcggcgcg ctgatcgact
     3361 tcgggcgctg gatcggcgcc cgccagcgac gcaagtaagc gcaggtgatc cgcagggatc
     3421 gcggccgggc gggacccggc gcgaggtcaa ccgctcagtg ccacttgccc ttgcccttgc
     3481 ccttgtgctt gtgtttgtgg tgccagccgt gctcgatgat gatcacgggc ggcggcgggg
     3541 ccgagtagac gtacacgccg acgaacaccg ggcgcgcggg caagaacagc cagccgtaga
     3601 acgggtgggt gtcgacgacg acgatcgtct cgcccttgac ctcgatcggg acgaagtact
     3661 cgtacacgat cgtgtcgtag ccgatgacga tcggcgcgta ggtggccttg acgacgagct
     3721 tcttggtctc gacgtcgggc tcgaagtgga tgaaggcgat gatgacctca tcctccttct
     3781 tcttggtctt cgacgggatc acgtgcagct cgaagacctt ggcctggttc ggcttctgga
     3841 tctcgacgac ctggatcttg tcgatcttgc cgtcgccgtc gacgtcgacg gtgttgagct
     3901 tctccttcgg gtcgttgatc ttcttctcga gcgcggcc
//
LOCUS       DASWNJ010000923         2150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_399463, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000923 DASWNJ010000000
VERSION     DASWNJ010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399463"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1110
                     /gene="hemC"
                     /locus_tag="VGB85_15675"
     CDS             115..1110
                     /gene="hemC"
                     /locus_tag="VGB85_15675"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY0135524.1"
                     /translation="MTTGTTGQVPLDRPLRIGTRGSELALWQAHHVRDELRRHWGEGL
                     AIELVLISTEGDRVLDRPLNKIGGKGLFVNEIEDRLLANAVDLAVHSMKDLPGHLAPG
                     LAIVCTPPREDPRDALVLGPRLRAQFPGDVTLADLPAGSILGTTSLRRTALARRQNGG
                     LDIRNLRGNVPTRLRKLAAGEYDAILLATAGLVRLGLAEQIAARLDPEGFCPAACQGI
                     LALECREDDAWVRTIVAPMNDEAAATMAAGERAFLARLDGGCQVPMGCSATLVGDDSV
                     HLRGVVADPAGRPVFSGTREGPRSEAAKLGCELAETLLQLGADVVLRALAHGGPG"
     gene            complement(1215..>2150)
                     /locus_tag="VGB85_15680"
     CDS             complement(1215..>2150)
                     /locus_tag="VGB85_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135525.1"
                     /translation="QLLAGADLDPTEIKGAVLGPWPAARGYVKTATGRPLAAFDTDDL
                     GRVRTFIDEAVYREQFAQLVPRAVEASRSLIDWVWPSWPELLYDAAAGNLDLEIAAEL
                     QSPELLVFTQTAEGLRTIKQKVRLKPGVRNRVANLPKLQADERTVVVLRARRATGEPL
                     LLEQALGAESKTFGVVPAAYVPPPPPPPPAELETSEPEPAAAPEASPEGEPVDPATTP
                     APGTAEDPTKPAATPKSSDPAGKASGTGATTKPATGTQPTGTSTKPATATQPTGTSTK
                     PATGTQPTTSAQPTGSATRPAASTASKPAASQPDK"
BASE COUNT          254 a          678 c          903 g          315 t
ORIGIN      
        1 tcatgatctc gcgacggccg tgcaggcgct gttcgggctc gagatccagg ccgacgggac
       61 cccggtcgcc gcgaccgagg gcgtgccggc ccccacgact tgaaggacat gcaattgacc
      121 acaggtacaa ccggacaggt cccactggac aggccgctga ggatcgggac gcgcggctcg
      181 gagctggcgc tgtggcaggc gcaccacgtg cgcgacgagc tgcgccggca ctggggcgag
      241 gggctggcga tcgagctggt gctgatctcg accgagggcg accgcgtgct cgatcgaccg
      301 ctcaacaaga tcggcggcaa ggggctgttc gtcaacgaga tcgaggaccg gctgctggcg
      361 aacgcggtcg acctcgcggt ccacagcatg aaggacctgc cgggccacct cgcgccgggg
      421 ctggcgatcg tgtgcacgcc gccgcgcgag gacccgcgtg acgcgctggt cctcgggccg
      481 cggctgcgtg cgcaatttcc gggggacgtg actctggcgg acctgccggc gggcagcatt
      541 ctcggcacga cgagcctgcg acgcaccgcg ctggcgcgcc ggcaaaacgg cgggctcgac
      601 atccgcaacc tgcgaggcaa cgtgccgacg cgcctgcgca agctcgcggc cggggagtac
      661 gacgcgatcc tgctggcgac cgccggcctg gtgcggctcg ggctcgcgga gcagatcgcg
      721 gcgcgcctcg acccggaggg cttctgtccg gcggcgtgtc aggggatcct cgcgctcgag
      781 tgccgcgagg acgacgcgtg ggtgcggacg atcgtggcgc cgatgaacga tgaggcggcg
      841 gcgacgatgg cggccggcga gcgggcgttt ttggcgcggc tcgacggcgg ctgccaggtg
      901 ccgatgggct gcagcgcgac gctcgtcggc gacgacagcg tgcacctgcg cggcgtcgtg
      961 gcggatcccg cggggcgacc ggtgttctcc gggacgcgcg aggggccgcg cagcgaggcg
     1021 gcgaagctcg ggtgcgagct ggcggagacg ctgttgcagc tcggggcgga cgtcgtgctc
     1081 agggcgctcg cgcacggcgg gcccggctga ggcgggtcag tcgcggtacg gggcgcggtc
     1141 gggcagtggt tgcatgcgct ctggcgtgtg aacccagccc ggctggtcgg gcttgcgctc
     1201 gtgcgccgcg ggtctcactt gtcgggctgc gacgcggccg gcttgctggc ggtactcgca
     1261 gcaggcctag tggcggagcc ggtcggctgt gcggacgtgg tcggctgcgt gccggtggcg
     1321 ggcttggtcg aggtgccggt cggctgcgtg gcagtggcgg gcttggtcga ggtgccggtc
     1381 ggctgcgtgc cggtggcggg cttggtcgtc gcgccggtgc ccgacgcctt gccagcgggg
     1441 tccgatgact tcggggtcgc ggccggcttc gtgggatcct cggctgtgcc gggtgcgggc
     1501 gtcgtcgcag ggtcgacggg ctcgccttcg ggtgatgctt cgggcgcggc cgcgggttcg
     1561 ggctcggagg tctcgagctc ggcgggcggt ggcgggggcg gtggcgggac gtaggcggca
     1621 gggacgacgc cgaaggtctt gctctcggcg ccgagggctt gctcgagcaa caggggctcg
     1681 ccggtggccc ggcgcgcgcg caggacgacg acggtgcgct cgtcggcctg caacttcggc
     1741 aggttggcga cgcggttgcg cacgccgggc ttgaggcgga ccttttgttt gatcgtgcgc
     1801 aggccctcgg cggtctgcgt gaacaccagc aattcggggc tctgcaattc ggcggcgatc
     1861 tcgaggtcga ggttgccggc ggcggcgtcg tacagcagct cgggccagga cggccatacc
     1921 cagtcgatca gcgagcgcga ggcctcgacg gcgcggggga ccagctgggc gaactgctcg
     1981 cggtagacgg cctcgtcgat gaaggtgcgc acgcggccga ggtcgtcggt gtcgaaggcg
     2041 gcgagcgggc ggccggtcgc ggtcttgacg tatccgcggg cggccggcca cgggccgagc
     2101 acggcgccct tgatctcggt cgggtcgagg tcggcgccgg cgagcagctg
//
LOCUS       DASWNJ010000924         2141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_582421, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000924 DASWNJ010000000
VERSION     DASWNJ010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582421"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VGB85_15685"
     CDS             complement(<1..524)
                     /locus_tag="VGB85_15685"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEY0135526.1"
                     /translation="MEGSQMAKQSAITDDGFRLSDELWEQMEPLLPARPAHPLGCHRP
                     RTPDRTAMDAIFFVLRTGCQWNALNATGICSSSSAHRRFQEWTEAGVFEAFWQAGLLA
                     YDGLEGIDWKWLALDGAMGKAPLGGGKNRSQPDGPGQVRDQAQPAHRRARHAALRRRG
                     WRQPQRPHPDGRDAG"
     gene            complement(574..1947)
                     /locus_tag="VGB85_15690"
     CDS             complement(574..1947)
                     /locus_tag="VGB85_15690"
                     /inference="COORDINATES: protein motif:HMM:NF024935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0135527.1"
                     /translation="MLPLPAHFAGLILAFAPLFVQRSWRHAHLLLVGAILTPGRRTVT
                     SVLRIMGRAQERRFVNVHRILNRAAWCPRSGSRILLGLLVNAFAQRGPVILGLDDTIE
                     RRRGKRIMAKGIYRDPVRSSDSHFVKASGLRWMSLMLLAPIPWAGRVWALPFLTALVP
                     SERACRERGRRHKPLLDVGRQLALQARRWLPGRDLVLVGDGGFSALLFLDAMRRARIT
                     AITRLRLDAALYNPAPPRPPGTIGRPRTKGARLPTLAATLASKDTRWHAIVVPGWYGA
                     GERTIEIASDTAVWRHGGLPVVPIRSRAHPRSRGALPAPGPALHRTDARARADRRMVR
                     AALERRGHVPGSARPSRRRDAAAVVRQGDCPHHALPTRPVLDRHAAGCTAPGPPTATH
                     RGRRVVPQTAPDLRRCARRRALCDLAREDFGDITAPTRPNKTPLPIACALAVCALPRC
                     MNGQSRA"
BASE COUNT          360 a          704 c          746 g          331 t
ORIGIN      
        1 ccagcgtctc gcccatcagg gtgtggtcgt tgcggttggc gccagccacg acgacggaga
       61 gcggcgtgcc gcgcgcgtcg gtgagcaggc tgcgcttggt cccgcacttg gcccggtccg
      121 tcgggttggg accggttttt ccccccaccg aggggcgcct tgcccatcgc cccgtccagc
      181 gccagccact tccagtcgat cccttccagc ccatcgtagg ccagaagccc tgcttgccag
      241 aacgcctcga acacacctgc ctcggtccat tcctgaaagc ggcgatgggc ggaggaggac
      301 gagcagatcc cggtcgcgtt cagggcattc cactggcagc cggtgcgtag cacgaagaag
      361 atcgcatcca tcgccgtccg gtcgggcgtg cgcggtctat ggcagcccag aggatgcgcc
      421 ggccgcgccg gtagtagtgg ctccatctgc tcccaaagct cgtctgagag acgaaagccg
      481 tcgtccgtga tcgctgactg cttggccatc tgactgccct ccatcctgga agacagatag
      541 gcccgctcgt ctctgcgcct actaggatag gttctaagct cgactttggc cattcatgca
      601 gcgtggcaga gcgcatacag ccagggcgca ggcaatcgga aggggtgttt tgttcgggcg
      661 cgtcggcgcg gtgatgtcgc caaagtcctc tcgcgccaga tcgcacagcg cacggcggcg
      721 agcgcatcgg cgaaggtcgg gcgcggtttg gggtaccacg cggcggccgc gatgcgtcgc
      781 cgttggcggg ccgggagccg tgcagccagc agcgtgacga tcgagaacag ggcgagtagg
      841 cagggcgtgg tgcgggcaat cgccttgtcg gaccactgcc gctgcgtctc gacgccgaga
      901 tgggcgcgca cttcctggaa cgtgacctcg acgttccagc gctgcacgaa ccatcctacg
      961 atctgcgcgg gctcgcgcgt cggttcggtg cagagcaggg cctggggcgg gaagcgcgcc
     1021 tcgggatcgc ggatgagcac gagagcggat cggtacgacg ggcaggccgc cgtgccgcca
     1081 caccgcggtg tccgaggcga tctcgatcgt gcgctcacct gctccgtacc agcccggcac
     1141 cacaatcgca tgccagcgcg tgtccttgga cgcgagggtc gcggcgagcg tcggcagccg
     1201 tgcgcctttg gtccgcgggc gtccgatcgt tccaggcggc cgcggcgggg cggggttgta
     1261 gagcgctgcg tcgagccgca ggcgggtgat cgctgttatg cgggcgcggc gcatcgcgtc
     1321 gaggaacagc aaggccgaga agccgccatc accgaccaac acgaggtcgc gccccggtag
     1381 ccagcggcgg gcctggaggg cgagttgacg tccgacgtcg agcaacggct tgtgccgacg
     1441 acctcgttcg cgacaggcac gctcggacgg cactagcgcg gtcaggaacg gcagcgccca
     1501 gacacgcccc gcccaaggga tcggggcgag cagcatcagg ctcatccagc gcagcccgct
     1561 cgccttgacg aaatggctgt cggaggagcg aaccggatca cggtagatgc ccttggccat
     1621 gatccgcttg ccgcggcggc gctcgatcgt gtcgtccagt cccaggatca cggggccgcg
     1681 ttgcgcgaac gcgttgacca ggagcccgag caggatgcgg ctgccgctgc gtgggcacca
     1741 cgctgcccgg ttgaggatgc ggtgaacatt cacgaaccgc cgctcctgcg cgcgacccat
     1801 gatgcgtagg acactcgtca ccgtgcgccg acccggcgtc aggatcgccc cgaccagcaa
     1861 cagatgggca tgccgccacg agcgttggac gaatagcggc gcgaaggcca ggatgagccc
     1921 ggcgaagtgg gcaggtaggg gcagcatggc ggcgtctctt ggcgaggatg caaccagcct
     1981 acctgtcact cccgcttcgt caccccacac cctcgacgac ccgtcccatt ggccaaagtc
     2041 gaggtaagag gacgtaccgt atggagggca cggccttaac ggacctcgca tgtccgacaa
     2101 acgatcggat acccgacggg cttactgata ggacgatgtc t
//
LOCUS       DASWNJ010000925         4150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_652755, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000925 DASWNJ010000000
VERSION     DASWNJ010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652755"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(429..683)
                     /locus_tag="VGB85_15695"
     CDS             complement(429..683)
                     /locus_tag="VGB85_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135528.1"
                     /translation="MTTLLASTNAWAAPAADNDADASAAKGRGASGTTVYDFDDDNVD
                     GELLSPEGANIASRGRTKHASLITIRPHFIPELIKMAQDV"
     gene            complement(1031..2521)
                     /locus_tag="VGB85_15700"
     CDS             complement(1031..2521)
                     /locus_tag="VGB85_15700"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0135529.1"
                     /translation="MRNLIKLASIGVLAVGLTACGKKNNGTNAPGDGPDNTAAPGKVD
                     PANPKAASAEAKADFQAAVKTYLSAKSDGKVAGEECDRVAGAFQRVYKQHGAQMAIAQ
                     FNSGVVYEECGQADKAEPIYSGLISSVPKFDLAYNNLGVIYWKKGQDAKALEMFKKGV
                     EANKLSARAARNNVAGLMRNQYVTNTDLNVFNEAQTAIQGVLALDSGNQAAYENLARL
                     YYDRGHLKDKSYLVLADLVVTQGLRVLKTENKESSDILNIKGLLLLEKDNQVDALKTF
                     KEAVRIEPNHVEANLNIGFISIRFRDYATAEGAFNTALKAPSVQKNIEVYLALGVAQR
                     GLKKFKEAEASYNKAKGLSANDPRPWFNLGVLYQDHLSTQDGVDPMKEGEKLVNTAKS
                     HYNKFISTAGGNKAYATRVLEAKDRIATIEQTIEFNKISAELEKKAAEMAKVAAAQEA
                     EERKRLLELEKQAMEAEDGGGAAPAPPAGGAAPAGGAAPPATAPKK"
     gene            complement(2533..>4150)
                     /locus_tag="VGB85_15705"
     CDS             complement(2533..>4150)
                     /locus_tag="VGB85_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135530.1"
                     /translation="AAGDPDGFRKCAEQFLSLYNEYGDEHEKASTLIYNAGECYEAAY
                     LLGQSVRMRKLLIDKFPASEHFQGTLSKLAGNYQAIAMYDKAAERMEAYAEKYVKDKE
                     SPDFLRNAYLFRLGLGQSDQANANLAKYEGLYKKDNPELAAKIFWSKHDILKTDDEKL
                     KHAEEYLKTYGKSGGLDRRIVAEASIGQILWRQSCDKGLLYDSCMTIERKVAQAGEKA
                     RQRAAELKRKDKAIKAKKASGSGKTKQEVPKNCGAATSGIVKVFPRNKKRADEAQKYF
                     DSVIKNAKAKISIPEDDRQRAEDFRNAVAMAQVYRADVQYEEYVVIAMPSGLSFFVEE
                     YKKDSGLPKWEKEYKEQVKKRDDSVKRFTEFYQKKGKLGQELQKTYDSIVEAKQSPYW
                     MLAAAARSAMVSQNYADQLYRAEVPAEFKTEEEAYAFCDELSDKAQPHQDAAVAKFSY
                     CLEKSTTFQFFNDFSRLCEEELQQRDADKYPSTSEIFGKSQYTASRPDRVDVQTDLEG
                     ERRKPDASKPAAEKKADDTAKKDEASDAAE"
BASE COUNT          567 a         1318 c         1390 g          875 t
ORIGIN      
        1 gtcgcccgag acctcgatca ccgcatgcat gcgcgccacg gcctcgtcat cgaggcacac
       61 gtggctgctc ttcagcttgc cgatcttgat gacgtcctgg gacagggtct gcgtatcgac
      121 gagctgatcg ccgtaaaaaa ccttgattgc gagggttttg gtggacatgg tcggggtcct
      181 tgggccagcg ggttacgttg gcagagacag cgggctgcag cagaggttcc gcggtttgga
      241 atatttttct ttcgacccag atgcgcgagg gcgcgcctgc cagcgcgcat atgggcgcac
      301 gcaagtcagg gcgggccggc cgaggcttgc gtgctgcgaa agacaatctt cacgggagaa
      361 tcgatgggcc cgccaggcgc tcgccggcgc cggagctgcg ccccgacgct tcgacgagcg
      421 gtccaggact agacgtcctg ggccatcttg atgagctcgg ggatgaagtg gggacggatg
      481 gtgatcaagc tggcgtgctt ggtccggccg cgggaggcga tgttggcacc ctcgggggac
      541 agcagctcgc cgtcgacgtt gtcgtcgtcg aagtcgtaga ccgtcgttcc gctggcacca
      601 cggcccttgg cggccgaagc gtcggcgtcg ttgtcggcag ccggcgcggc ccaggcgttc
      661 gtggaggcca ggagtgtcgt catcagggtg accattgcga agcgcatgtg aacctcgatc
      721 ttctcgcgtg agaacaggga ctttcgcgca acatacctcg cgcccccccg gcttgtaaac
      781 cgcctcgcaa ggggcgcggg gggccgtttt ggcccgcgcg aggtcgctgg aacgcccgaa
      841 cccggaccgc cggtctgtgc cacggtcgag atctgccgcg atccggctcg ctccatcgcg
      901 gcgtcgggcg cggccccttc gtttccgggc cccgcccaga ctggcaatcc ccacgaacgc
      961 ccaaattggc gagagggtcg ccggccgaag ccgggaccct ctcggtgtgc tcacaggaga
     1021 gatcccgcga tcacttcttg ggcgcagtgg ccgggggagc cgcgccacct gccggtgccg
     1081 caccacccgc cggcggagcc ggggccgcgc cgccgccgtc ctcggcctcc atcgcctgct
     1141 tctcgagctc aagcagacgc ttgcgctcct cggcctcttg cgccgccgcg accttggcca
     1201 tctcggcggc cttcttctcg agttcggcgc tgatcttgtt gaactcgatc gtctgctcga
     1261 tggtggcgat gcggtccttc gcctcgagca cgcgggtcgc gtacgccttg ttgccgccgg
     1321 cggtcgagat gaacttgttg tagtggctct tggccgtgtt cacaagcttc tcgccctcct
     1381 tcatcgggtc gacgccgtcc tgcgtggaca ggtgatcctg gtacagcacg ccgaggttga
     1441 accacgggcg cgggtcgttc gcgctcaggc ccttggcctt gttgtacgag gcctctgcct
     1501 ccttgaactt cttgaggccg cgctgggcga ccccgagggc cagatacacc tcgatgttct
     1561 tctgcaccga gggggccttg agcgccgtgt tgaacgcgcc ttcggccgtc gcgtagtcgc
     1621 ggaagcggat cgagatgaag ccgatgttga ggttggcctc gacatggttc ggctcgatgc
     1681 gtaccgcctc cttgaaggtc ttgagcgcgt cgacctggtt gtccttctcc aacaagagca
     1741 ggcccttgat gttgaggatg tccgagctct ccttgttctc ggtcttgagg acgcgcagac
     1801 cctgggtgac gacgaggtcc gcgagcacga ggtacgactt gtccttgagg tgaccgcggt
     1861 cgtagtagag gcgcgccagg ttctcgtagg cggcctggtt gccggagtcg agcgccagca
     1921 cgccctggat cgccgtctgc gcctcgttga acacgttgag gtcggtgttc gtgacgtact
     1981 gattacgcat cagtccggcg acgttgttgc gagccgcgcg ggcgctgagc ttgttggcct
     2041 cgacgccctt cttgaacatc tcgagggcct tggcgtcctg gcccttcttc cagtagatga
     2101 cgccgaggtt gttgtaggcg aggtcgaact tcggcaccga gctgatgagg cccgagtaga
     2161 tcggctcggc cttgtcggcc tggccgcact cctcgtagac gacgcccgag ttgaactggg
     2221 cgatcgccat ctgcgcgccg tgctgcttgt agacgcgctg gaacgcacca gcgacgcggt
     2281 cgcactcctc gccggcgacc ttgccgtcgc tcttggccga cagataggtc ttcacggccg
     2341 cctggaagtc ggccttggcc tcggccgaag ctgccttcgg gttggccggg tcgaccttgc
     2401 caggggccgc agtgttatcg ggaccatcac cgggggcgtt ggtgccgttg ttcttcttgc
     2461 cacaagccgt cagaccgaca gccaggacac cgatgcttgc gagtttgatc aggttacgca
     2521 tgggtggttc ctctactcgg ccgcgtcgct ggcctcatcc ttcttggcgg tgtcgtcggc
     2581 cttcttctcg gccgcgggct tgctggcatc gggcttgcgc cgctcgccct cgaggtccgt
     2641 ctggacatcg acgcgatcgg ggcggctcgc ggtgtactgc gactttccga agatctcgga
     2701 ggtcgacggg tacttgtcgg cgtcgcgctg ctggagctcc tcctcgcaca ggcgcgagaa
     2761 gtcgttgaag aactggaagg tcgtcgactt ctcgaggcag tacgagaact tggcgaccgc
     2821 cgcgtcctgg tgcggctggg ccttgtcgct cagctcgtcg cagaaggcgt aggcctcctc
     2881 ctcggtcttg aactccgccg gcacctcggc gcggtagagc tggtcggcgt agttctgcga
     2941 gaccatggcc gagcgagcgg ccgcggcgag catccagtac ggcgactgct tggcctcgac
     3001 gatcgagtcg taggtctttt gcagctcctg accgagcttg cccttctttt gatagaactc
     3061 ggtgaagcgc ttgaccgaat cgtcacgctt cttgacctgc tccttgtact ccttctccca
     3121 cttggggagc ccggagtcct tcttgtactc ctcgacgaag aagctgaggc cggagggcat
     3181 cgcgatgacg acgtactcct cgtactggac gtcggcgcgg tagacctgcg ccatggcgac
     3241 cgcgttgcgg aagtcctcgg cgcgctgacg gtcgtcctcg gggatcgaga tcttggcctt
     3301 ggcgttcttg atgacgctgt cgaagtactt ctgcgcctcg tcggcccgct tcttgttgcg
     3361 cgggaacacc ttgacgatgc ccgaggtggc ggcgccgcag ttcttcggga cctcctgctt
     3421 ggtcttgccg ctgccgctcg ccttcttggc cttgatcgcc ttgtccttgc gcttgagctc
     3481 ggcggcccgc tggcgggcct tctcgccggc ctgggcgacc ttgcgctcga tcgtcatgca
     3541 cgagtcgtag agcaggccct tgtcgcagga ctggcgccac aggatctggc cgatcgacgc
     3601 ctcggcgacg atgcggcggt cgaggccgcc cgacttgccg taggtcttga ggtactcctc
     3661 ggcgtgcttg agcttctcgt cgtcggtctt gaggatgtcg tgcttcgacc agaagatctt
     3721 ggccgcgagc tccgggttgt ccttcttgta gagaccctcg tacttggcca ggttggcgtt
     3781 tgcctggtcg ctctggccga ggccgaggcg gaacaggtag gcgttgcgca ggaagtcggg
     3841 cgactccttg tccttgacgt acttctcggc gtacgcctcc atgcgctcgg cggccttgtc
     3901 gtacatcgcg atcgcctggt agttgcccgc cagcttcgac agcgtgccct ggaagtgctc
     3961 gctcgccggg aacttgtcga tcagcagctt gcgcatacgg accgactggc cgagcaggta
     4021 ggcggcctcg tagcactcgc ccgcgttgta gatcagggtc gacgccttct cgtgctcgtc
     4081 gccgtactcg ttgtagagcg agaggaactg ctccgcgcac ttgcggaagc cgtccgggtc
     4141 gcccgcggcg
//
LOCUS       DASWNJ010000926         3137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_328502, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000926 DASWNJ010000000
VERSION     DASWNJ010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328502"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VGB85_15710"
     CDS             <1..214
                     /locus_tag="VGB85_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135531.1"
                     /translation="LVVARALPDGGRVGILNREFSVRGRDGHSERRALETPEELLAVL
                     AEHFGLVFPAGTVFPCEALDWPASSG"
     gene            complement(206..>3137)
                     /locus_tag="VGB85_15715"
     CDS             complement(206..>3137)
                     /locus_tag="VGB85_15715"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0135532.1"
                     /translation="ATLREMGIHSVYPTAGALVVTVRQAGSATDQAALAARVALLIDD
                     RWRAASVAVLTGRGMTRGNSTVGEVADRAALMLQRSPGDSSSLPHKDHSGVWLDELTA
                     RLLGPEFTVTRTGEYALLLGEEKDVDASRPLLGKPTPCVGRDSELGTLEAQLDACIAD
                     AEARAVIVTAPSGTGKSRLRHEFMRRLHKRDEPVTQVFGRGDMIGAGAPYGILAQALR
                     RFCGLGGGEAVDEQRRRLRTRLGRHLAPADQERVCACMLELVGVSPASSPTSRAAHMD
                     PALMHKEIRRAFLDWLAVETAAAPVLLVLDDLQWGDALTVALVDEALRKLEGAPLFVL
                     ALARPELHEVFPRLWQGHRAQEIALKGLGKKACERLIQQVLGKQVPAEVMARAVERSA
                     GNALFLEEMIRAIAEGKSSEPDEAPEAVLAILQARIGRFEVGPRRAVRAAAVFGQTFW
                     QGGVAAVLGQPRTGPGVEAWLATLVEQEVIERRADSRLGGEVEYGFRHALVQEVAYSL
                     LTESDLATGHQLAGEFLVEAGERDATQIAEHFVRAGVLDRAVTYYMKAGQEAVSRSLL
                     REARTSYAAAKATIERLPELPEHCRQHADILLMQVKTGVTNDPAEVNLQRIARARELL
                     ESVMRSGAALPEDRLRMARVEHYRARAQCYGGQQNLAEANLRALLPIAEEFRDHELAT
                     TAGFTLGLSLMLQGKFGQARDTFAGALPVMERLFGTSIDTMRCCMYYAMVLSASGRPR
                     EAAAQVARATRWAQALEQPMYTSVFHMTRMLGAALAADWPATLEAGEALGDASKQAGE
                     TMFLHTMLDGMSWAQSYLGQCDLAVANVERSAELKRSLGLNTIGQDWWDAAAADVLLN
                     AGRTEQALARAQQIAAGSQAIGLHFSHALAERVWACALSRLGGAAAEVDRHFATSLQV
                     ALPCEQTTSAIQTRLWWGRICRERGDEAAATDHFEQAIAALEASGHEFALDAARHLAG
                     MPSA"
BASE COUNT          420 a         1155 c         1130 g          432 t
ORIGIN      
        1 tctcgtcgtc gcgcgcgcgc tgcccgacgg cgggcgcgtc gggatcctca accgcgaatt
       61 ctcggtgcgc gggcgcgacg gccacagcga acgccgcgcg ctggaaacac ccgaggagct
      121 gctcgcggtg ctcgcggagc acttcggcct ggtgttcccc gcgggcaccg tgttcccctg
      181 cgaggcgctc gactggccgg cgagctcagg ctgaaggcat accggcgagg tggcgcgcgg
      241 cgtcgagggc gaactcgtgg ccgctcgcct cgagcgccgc gatcgcctgt tcaaaatggt
      301 cggtggccgc ggcctcgtcg ccgcgctcgc ggcagatccg tccccaccac aggcgggtct
      361 ggatcgcgct ggtcgtctgc tcgcagggaa gggccacctg caggctcgtg gcgaagtgac
      421 ggtcgacctc ggccgccgcg ccgccgagcc ggctcaacgc gcaggcccac acgcgttcgg
      481 cgagcgcgtg cgagaagtgc aggccgatcg cctgcgagcc cgccgcgatc tgctgcgcgc
      541 gggccagggc ctgctcggtg cgcccggcgt tgagcagcac gtccgcggcg gcggcgtccc
      601 accagtcctg accgatcgtg ttgaggccga gcgaccgctt cagctccgcg gagcgctcga
      661 cgttggcgac ggcgaggtca cactggccga ggtagctctg ggcccacgac atcccgtcga
      721 gcatcgtgtg caggaacatc gtctcgccgg cctgcttgct cgcgtcgccg agcgcctcgc
      781 cggcctcgag ggtggccggc cagtccgcgg cgagggcggc cccgagcatg cgcgtcatgt
      841 gaaacacgct cgtgtacatc ggctgctcca gcgcctgggc ccagcgggtc gcgcgggcga
      901 cctgcgcggc ggcctcgcgc gggcggcccg acgcggacag gaccatcgcg taatacatgc
      961 agcagcgcat ggtatcgatg ctcgtgccga agaggcgctc catgaccggc aacgcgccgg
     1021 cgaaggtatc gcgcgcctgg ccgaacttgc cctgcagcat cagcgagagg ccgagggtga
     1081 acccggcggt ggtcgccagc tcgtgatcac ggaactcctc ggcgatcggg aggagcgcgc
     1141 gcaagttggc ctcggcgagg ttctgctggc cgccgtagca ctgcgcgcgg gcccggtagt
     1201 gctcgacgcg ggccatccgt agccggtcct cgggcagcgc ggcgcccgag cgcatcacgc
     1261 tctccagcag ctcacgcgcc cgggcgatgc gctggaggtt gacctcggcc gggtcgttgg
     1321 tgaccccggt cttgacctgc atcaataaaa tatcggcgtg ttgtcggcag tgctcgggca
     1381 gctcgggcaa ccgctcgatc gtcgccttcg cggccgcgta ggaggtccgc gcctctcgca
     1441 gcagcgagcg cgagacggcc tcctgtccgg ccttcatgta ataagtgacc gcccggtcga
     1501 gcacgccggc gcgcacgaag tgctcggcga tctgcgtcgc gtcgcgctcg ccggcctcca
     1561 cgaggaactc accggcgagc tggtggccgg tggcgaggtc gctctcggtc agcaacgagt
     1621 acgcgacctc ctgcaccagc gcgtggcgaa atccatactc gacctcgccg ccgagccgac
     1681 tgtcggcgcg gcgctcgatc acctcctgct cgaccagcgt ggcgagccag gcctcgacgc
     1741 cggggccggt gcgtggctgg ccgagcacgg ccgccacgcc gccttgccag aaggtctgtc
     1801 cgaataccgc cgcggcgcgc acggcccggc gcgggccgac ctcgaaccgg ccgatgcgcg
     1861 cctgcaagat cgccagcacg gcctcgggcg cctcgtcggg ctcggacgac ttgccctcgg
     1921 cgatcgcgcg gatcatctcc tcgaggaaca gcgcgttgcc ggccgagcgc tcgaccgctc
     1981 gcgccatgac ctcggcgggg acctgcttgc cgagcacctg ctggatcagc cgctcgcacg
     2041 ccttcttgcc gaggcccttg agcgcgatct cctgcgcgcg gtggccctgc cacagccgcg
     2101 ggaacacctc gtgcagctcc gggcgcgcca gcgcgagcac gaacagcggc gccccctcga
     2161 gcttgcgcag cgcctcgtcg acgagcgcga cggtcagcgc gtcaccccat tgcaggtcgt
     2221 cgagcaccag cagcaccggc gccgcggcgg tctcgaccgc gagccagtcg aggaaggcgc
     2281 ggcggatctc cttgtgcatc agcgcgggat ccatgtgcgc cgcgcgggag gtcggcgagc
     2341 tggccggcga tacgccgacg agctcgagca tgcacgcgca cacgcgctcc tggtccgcgg
     2401 gcgcgaggtg ccggccgagg cgcgtacgca gccgccggcg ctgctcgtcg accgcctcgc
     2461 cgccgccgag gccacagaac cggcgcagcg cctgcgcgag gatcccatac ggcgcgccgg
     2521 cgccgatcat gtcgccgcgg ccgaacacct gcgtcaccgg ctcgtcgcgc ttgtgcaggc
     2581 gccgcatgaa ctcgtggcgc agccgcgact tgccggtgcc ggacggcgcc gtgacgatca
     2641 ccgcccgcgc ctcggcgtcc gcgatgcagg cgtcgagctg cgcctcgagc gtgccgagct
     2701 cgctgtcgcg gccgacgcag ggggtcggct tgccgagcag cggccggctc gcgtcgacat
     2761 ccttctcctc gccgagcaac agcgcatatt cgccggtccg cgtcacggtg aattccgggc
     2821 cgagcaggcg cgccgtcaat tcgtcgagcc agaccccgga gtggtccttg tgcggcagcg
     2881 acgagctgtc gccgggcgag cgttgcagca tcagcgcggc gcggtcggcc acctcgccga
     2941 cggtcgagtt gccgcgcgtc atgccacgtc cggtcaacac ggcgaccgag gcggcgcgcc
     3001 agcggtcgtc gatcaacagg gccacgcggg cggcgagggc cgcctggtcg gtcgcgctgc
     3061 cggcctgtcg caccgtcacg acgagcgcgc ccgcggtcgg atataccgag tggatcccca
     3121 tctcgcgcag cgtggcg
//
LOCUS       DASWNJ010000927         2315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_17147, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000927 DASWNJ010000000
VERSION     DASWNJ010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17147"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="VGB85_15720"
     CDS             <1..965
                     /locus_tag="VGB85_15720"
                     /inference="COORDINATES: protein motif:HMM:NF018031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferric iron reductase"
                     /protein_id="HEY0135533.1"
                     /translation="VTHKAMAAGASVWDAFLASVDALLRAGVDPHARRGLRLQHEPAS
                     AGLDPAALGGRNDLAGSLGVIFRDDLRGPTAALGDEAGEAGEAVAWVCAALGERWPGV
                     PGDMSSGPGSTTSAAGTLLHRSEGDPLLRAITSAYPTPMLALRHYVELLVPPALRLCT
                     AHGVALEMHLQNTLVVHRGGRLCGFIVRDLGGIRIHRGRLTRAGHAPDLAAGSFTVTA
                     DLAETQTKLAHTLLHAHLGTVFAWAADLLGADEAALWAHTRAVIEGCLAAWAQAQPQL
                     AEACAQDRAVLLAPVVQAKALLRMRIDERISDYAYTRVSGPFAP"
     gene            complement(978..>2315)
                     /locus_tag="VGB85_15725"
     CDS             complement(978..>2315)
                     /locus_tag="VGB85_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135534.1"
                     /translation="APRPRLSWTFDHPLDPHWRIDDPMALAREPGTGALHMHSSLAKT
                     IARLPLRHDGGTFGVSVEIAVDQVEWGSTIEVKLHGGAVTTGVRVFGEGSVEAPDFAY
                     RGLHPHEAVVLPKVPAGRIRLDHTIYPELELQTVTLEIPGTPKQRHVRPVDVAITPGA
                     MTFELIATSTSPLLVGHARIHRIELHGLELDPAGHDAAGAPDQALARAIVEGELTAAL
                     ALLPPPGALAPGDLRLLWRSELLARLGDLDGAAAALRRGLDVPEDSPFNAAFTLQLHT
                     TRGPWQLVVDRVLGPKALWFLHDLTALSHPWRPEAVGDALARLAAWPREPVASDSRRL
                     SDQALALIVRGEAWRRFGRPDLARPDLDAAAAIVRRPGLEIPRQMIDELQQRRLELAA
                     RTGDRERALELMTAMIREAPSPEIRLERFRGREELVALLRPEDWAALAALTRE"
BASE COUNT          285 a          861 c          875 g          294 t
ORIGIN      
        1 cggtcacgca caaagccatg gccgcgggcg ccagcgtctg ggacgcgttt ctggccagcg
       61 tcgacgcttt gctgcgcgcc ggcgtcgacc cgcacgcgcg ccggggtctg cggctgcaac
      121 acgagccggc ctccgccggc ctcgaccccg cggcgctcgg cggccgcaac gacctcgccg
      181 gcagcctcgg cgtcatcttt cgcgacgacc tgcgcgggcc caccgcggcg ctcggcgatg
      241 aggcaggtga agcgggcgag gccgtcgcgt gggtgtgcgc cgcgctcggc gagcgctggc
      301 cgggtgtccc cggggacatg tcctcggggc caggctcgac cacctctgcc gccgggaccc
      361 tgctgcaccg cagcgagggc gacccgctgc tgcgcgcgat cacctcggcc tatccgaccc
      421 cgatgctcgc gctgcgtcac tacgtcgagc tgctggtccc gccggcgctg cgcctgtgca
      481 cggcgcacgg cgtcgcgctc gagatgcacc tacaaaacac gctcgtcgtg caccgcggcg
      541 gccggctgtg cggcttcatc gtccgcgacc tcggcgggat ccggatccac cgcggcaggc
      601 tgacgagggc cggccacgcg cccgacctcg ccgccgggtc gttcaccgtc accgccgacc
      661 tcgccgagac ccagaccaag ctcgcgcaca cgctgctgca cgcgcacctc ggcaccgtct
      721 tcgcgtgggc cgccgacctg ctcggcgccg acgaggccgc gctgtgggcc cacacgcgcg
      781 cggtgatcga gggctgcctc gccgcgtggg cgcaggcgca gccgcaattg gccgaggcgt
      841 gcgcgcagga ccgggccgtg ctgctcgcgc cggtcgtgca ggccaaggcc ctgctgcgca
      901 tgcgcatcga cgagcggatc agcgactacg cgtacacccg cgtgtccggt ccgttcgcgc
      961 cgtgaggcgc ccgcgggtca ctcgcgggtg agcgcggcga gggcggccca gtcctcgggg
     1021 cgcagcaggg cgacgagctc ctcgcgcccg cgaaaccgct cgagccggat ctcgggcgac
     1081 ggcgcctcgc ggatcatcgc ggtcatcagc tcgagcgcgc gctcccggtc gccggtgcgg
     1141 gccgccagct cgaggcggcg ctgctgcagc tcgtcgatca tctggcgcgg gatctcgagg
     1201 ccggggcggc gcacgatcgc cgccgcggcg tcgaggtcgg ggcgcgccag gtcggggcgg
     1261 ccgaagcgac gccaggcctc cccgcggacg atcagcgcga gcgcctggtc ggacagccgc
     1321 cggctgtcgc tggcgaccgg ctcgcgcggc cacgcggcca ggcgcgccag cgcgtcaccg
     1381 acggcctcgg gccgccacgg gtgggacagc gcggtgaggt cgtggaggaa ccacagcgct
     1441 ttcggcccga gcacacgatc gacgacgagc tgccacgggc cgcgggtcgt gtgcagctgc
     1501 aacgtgaacg cggcgttgaa cgggctgtcc tcggggacat ccaggccgcg tcgcagggcc
     1561 gccgccgcgc cgtcgaggtc gccgagtcgg gcgagcagct cggagcgcca cagcaggcgc
     1621 aggtcgcccg gcgcgagcgc tccaggcggc ggcagcaggg cgagcgccgc ggtcagctcg
     1681 ccctcgacga tcgcccgcgc gagcgcctgg tccggcgcgc ccgcggcgtc atgcccggcg
     1741 ggatcgagct ccaggccatg cagctcgatg cgatggatcc gggcgtggcc gaccaggagc
     1801 gggctcgtgc tcgtggcgat cagctcgaag gtcatcgcgc ccggcgtgat cgcgacgtcg
     1861 accggtcgca catggcgctg cttcggcgtg ccggggatct ccagcgtgac cgtctgcagc
     1921 tcgagctcgg ggtagatcgt gtgatcgagg cggatccgtc ccgcggggac cttcgggagc
     1981 acgacggcct cgtgcgggtg aaggccgcgg tacgcgaagt ccggcgcctc gacgctaccc
     2041 tcgccgaaca cgcggacccc ggtcgtcacg gccccgccgt ggagcttgac ctcgatggtg
     2101 ctgccccact cgacctggtc gaccgcgatc tcgacgctca cgccgaaagt gccgccgtcg
     2161 tgtcgcagcg gcaggcgagc gatggtcttt gcgaggctgc tgtgcatatg cagcgcgccg
     2221 gtgccgggct cgcgcgcgag cgccatcggg tcgtcgatcc gccagtgcgg gtcgagcgga
     2281 tggtcgaagg tccacgacag tcgcgggcgc ggggc
//
LOCUS       DASWNJ010000928         1879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_157998, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000928 DASWNJ010000000
VERSION     DASWNJ010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157998"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VGB85_15730"
     CDS             complement(<1..541)
                     /locus_tag="VGB85_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135535.1"
                     /translation="MPYDRAHVISSPVSALALVALALTTACGDDTIDSTGTDATTTAT
                     STTSATSVTDTTAPTGEPTTTSPTSTTTDLTTGTTGPSTGPDPTSDTTTDTTGPITAT
                     EPGTTTTDTAGTDTDDSNGSLTTDTTGDTGTSTGDEPCAQGTVLCEGMVAKVCDGMGG
                     FASEETCPKVCTPGIGCTEC"
     gene            665..>1879
                     /locus_tag="VGB85_15735"
     CDS             665..>1879
                     /locus_tag="VGB85_15735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135536.1"
                     /translation="MRPSSLLLACSLGIVLAACNGSAGSEGDGSTSGASTSGGTPTTG
                     DVTPTGGSTDGTGSDTAEDPTTGGPWELGECNPVDPTMCALPYPSLFHMREADTPTGW
                     QLAYTAAALPKNNSGTTMDPKYLNEKDGVSVLGALLFSFPNVDLTGVLTHLTLADYMK
                     EDVKTVLLDAETGERVAHFVELDMRTQAADQRLIVVQPATPLRHGKRYVFAARGLGKQ
                     GGGQVEASPGFAALRDKVGTDDMSVEGQRVHYNLDIFPLLADAGFAREELQIAWDFVT
                     VSRENSLGRMEWLRDDVLDAIGPDGPDYVIKSSVDDDCTNPEVAIGRTILVDMTTPLY
                     TTADEPGTLLTRGPDGMPFRNGTTEVEVLLRIPCSLIDQDEPRSGRVLQYGHGLLGGK
                     GEAQGGYLSRMAN"
BASE COUNT          262 a          619 c          719 g          279 t
ORIGIN      
        1 cacactcggt gcagccgatg cccggcgtgc acaccttggg gcaggtctcc tcgctcgcaa
       61 agccgcccat gccgtcgcac accttggcga ccatgccctc gcacaggaca gtgccctgcg
      121 cgcagggctc gtcgccggtg ctcgtgccgg tgtcgccggt ggtgtcggtg gtcagcgagc
      181 cgttgctgtc gtcggtgtcg gtgccggcgg tgtcggtggt cgtggtgccg ggctcggtcg
      241 cggtgatcgg accggtcgtg tcggtcgtcg tatcactcgt cgggtcgggg ccggtgctcg
      301 gacccgtcgt gccagtcgtc aggtcggtgg tcgtgctggt cgggctcgtg gtcgtcggct
      361 cgccggtggg cgcggtcgtg tcggtgaccg aggtggccga ggtggtcgag gtggccgtgg
      421 tggtcgcgtc ggtcccggtg ctgtcgatcg tgtcgtcgcc gcaggccgtc gtcagggcga
      481 gggcgacgag ggcgagggca gacaccggcg aagaaattac gtgggcgcgg tcgtaaggca
      541 tcgcaggact gtatcgggcc cggccggcgc gcggcagggg gggggaccgg gtgtctggac
      601 tcacgcgcgc ggaggcgcgg cgatggttcc aggtccggcg ccggcgcgat atgggtcgac
      661 gaagatgcga ccctccagcc tgctcctcgc ctgctccctc ggaatcgtgc tcgcggcctg
      721 taatggtagt gcggggagcg agggcgacgg ctcgacctcc ggcgcgtcga cgagcggcgg
      781 cacgccgacg acgggtgacg tcacgccgac cggcggctcg acggacggca ccggatccga
      841 caccgcggaa gatccgacga cgggcgggcc gtgggagctc ggcgagtgca acccggtcga
      901 cccgacgatg tgcgcgctgc cgtacccctc gctgttccac atgcgcgagg ccgacacccc
      961 gaccggctgg cagctggcgt acacggccgc ggcgctgccg aagaacaaca gcggcacgac
     1021 gatggacccg aagtacctca acgagaagga cggcgtgtcg gtgctcgggg cgctgctgtt
     1081 ctcgttcccg aacgtcgacc tgacgggcgt gctgacccac ctgacgctcg ctgattacat
     1141 gaaggaggac gtgaagacgg tgctgctcga cgccgagacc ggcgagcggg tggcgcactt
     1201 cgtcgagctc gacatgcgca cgcaggcggc cgaccagcgg ctgatcgtcg tgcagccggc
     1261 gacgccgctg cgccacggca agcgctacgt gttcgcggcg cgggggctcg gcaagcaggg
     1321 cggcgggcag gtcgaggcct cgccgggctt cgcggcgctg cgcgacaagg tcggcaccga
     1381 tgacatgagc gtcgagggcc agcgcgtgca ctacaacctc gacatcttcc cgctgctcgc
     1441 cgacgccggc ttcgcgcgcg aggagctgca gatcgcgtgg gacttcgtca ccgtgtcgcg
     1501 cgagaacagc ctcgggcgca tggagtggct gcgcgacgac gtgctcgacg cgatcggccc
     1561 cgacggcccc gactacgtga tcaagtcgtc ggtcgacgac gactgcacca accccgaggt
     1621 cgcgatcggc cgcacgatcc tggtcgacat gacgacgccg ctgtacacca ccgccgacga
     1681 gcccggcacg ctgctgacgc gcggcccgga cggcatgccg tttcgcaacg ggacgacgga
     1741 ggtcgaggtg ctgctgcgga tcccgtgctc gctgatcgac caggacgagc cgcgttccgg
     1801 ccgcgtgctc cagtacggcc atggtctgct cggcggcaag ggcgaggccc agggcggcta
     1861 cctgagccgg atggcgaac
//
LOCUS       DASWNJ010000929         2748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_369163, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000929 DASWNJ010000000
VERSION     DASWNJ010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369163"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VGB85_15740"
     CDS             <1..265
                     /locus_tag="VGB85_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135537.1"
                     /translation="GLLLVVMLVVVVMTGAGLGGWFGEQIARSLWATTMRELDGDGPL
                     RTDLQRWRALVRSLAHHRDLLAGGQRGLPFRCEFNAEPDSLPA"
     gene            424..1872
                     /locus_tag="VGB85_15745"
     CDS             424..1872
                     /locus_tag="VGB85_15745"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HEY0135538.1"
                     /translation="MTRASLSLLMSLGTGLLLAACGDDTGTSGSTASTGESTTDGPTS
                     MTEPTTNPTTGQTGDMTTGQTSGGMTESASSTVDPSTGEPTESGTTAETTTGNIGTTG
                     EPDTTTDQPETTTGVDTTTGADTTTGGDTTTGDTTTGEMTTGNAEECLLAQQHEPCDA
                     MSNDALHAMGLNCTSLGAMWTDNVNAVTVTGLDFQAPPPMNGERTWQVAKSYGSFIDP
                     DTKKPFWGPREGDKFLMISSGLLPTPNGQGAVIIANGDVYNDTAFGGVWDSDSMPPPM
                     SAQKGSPDPMGYTNCDGTNDCSNTILDQWNLGFSNAEDKMWFSFNLKAPAIANGDIAD
                     ANGYTFNFAYFSAEFPEYVDSQFNDIFVVWQSSEDYTGNVTFINGQPMTVTALWPIDF
                     QGGCDFFDPNCQGNDPHLAGTGHSMDGGATNWYKATGGIKPGETFLLAFSVFDMGDSS
                     FDTTALLDNWQWDCEGCVPNEIMGCGIEPEPM"
     gene            2070..>2748
                     /locus_tag="VGB85_15750"
     CDS             2070..>2748
                     /locus_tag="VGB85_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135539.1"
                     /translation="MTRSSLYLLLGAGLALTAACGDDNAGESGASASEGATDSATGVT
                     GVTGSATDADSATDPTTGMSASMSSTEDPTGASTTDVNVTEGSSSGGGDTEASTTGPV
                     DPGTTTDVETTDPGTTTDAETSTTGPDSTTGDSEECPLSLQHMPCDGASDDALHALGL
                     NCTTAGNQWINNDNAVAVAKLDFQAPPPSGGRRTWQVAKSYGTHVDPNTNKPFWGPRE
                     GEKVLLIS"
BASE COUNT          487 a          979 c          924 g          358 t
ORIGIN      
        1 cgggctgctg ctggtggtga tgctcgtcgt cgtggtgatg acgggggcgg gcctcggcgg
       61 gtggttcggc gagcagatcg cccgcagcct gtgggcgacg acgatgcgcg agctcgacgg
      121 cgacggcccg ctgcgcacgg acctgcagcg ctggcgggcc ctggtccgca gcctcgcgca
      181 tcaccgcgat ctgctggccg gcgggcagcg cgggctgccg ttccgctgcg agttcaacgc
      241 cgagcccgac tcgctgccgg cctgagggcc cgctgcgcct gagccaccgg atcggggatc
      301 gccctggacc ggggccccct gctgggccaa atcgctccgg gcccggcaat tttgacgctc
      361 ggaggtcggc gatcgtgtcc gggtccgctg gtgctcatgc gctcactccg ctaaggatcg
      421 ggtatgacac gcgcttccct ttctctactg atgagccttg gcaccggcct cctcctggcc
      481 gcttgcggcg acgacaccgg cacctcgggc tcgaccgcct ccaccggcga gagcacgacc
      541 gacggcccga cctcgatgac ggagccgacc accaacccga cgaccggtca gaccggcgac
      601 atgacgaccg gtcagaccag cggcggcatg acggagtcgg cgagctcgac cgtcgacccg
      661 agcaccggcg agccgaccga gtccggcacg accgccgaga cgacgaccgg caacatcggt
      721 acgacgggtg agcccgacac gaccaccgac cagcccgaga cgacgaccgg cgtcgacacg
      781 acgaccggcg ccgacacgac gaccggcggc gacacgacga ccggcgacac gacgaccggc
      841 gagatgacga ccggcaacgc cgaggagtgt ctgctggccc agcagcacga gccctgcgac
      901 gcgatgtcga acgacgccct gcacgcgatg gggctcaact gcacctcgct cggcgccatg
      961 tggaccgaca acgtcaacgc ggtcacggtg accggcctcg acttccaggc gcccccgccg
     1021 atgaacggcg agcgcacctg gcaggtcgcc aagtcgtacg gcagcttcat cgatcccgac
     1081 accaagaagc cgttctgggg cccgcgcgag ggcgacaagt tcctgatgat cagctcgggc
     1141 ctgctgccga ccccgaacgg gcagggcgcg gtgatcatcg ccaacggcga cgtgtacaac
     1201 gacaccgcct tcggcggcgt gtgggacagc gactcgatgc ccccgccgat gtcggcgcaa
     1261 aagggcagcc ccgatccgat gggctacacc aactgcgacg ggaccaacga ctgctccaac
     1321 acgatcctcg accagtggaa cctcggcttt agcaacgccg aggacaagat gtggtttagc
     1381 ttcaacctca aggcgcccgc gatcgccaac ggcgacatcg ccgacgccaa tggctacacc
     1441 ttcaacttcg cgtacttctc ggccgagttc ccggagtacg tcgacagcca gttcaacgac
     1501 atcttcgtcg tgtggcagtc gtccgaggac tacacgggca acgtcacctt catcaacggc
     1561 cagccgatga cggtcaccgc gctgtggccg atcgacttcc agggcgggtg cgacttcttc
     1621 gacccgaact gccagggcaa cgacccgcac ctcgcgggca ccggccacag catggatggc
     1681 ggcgcgacca actggtacaa ggcgaccggc ggcatcaagc cgggcgagac cttcttgctc
     1741 gccttctcgg tgttcgacat gggcgactcg agcttcgaca ccacggccct gctcgacaac
     1801 tggcagtggg actgcgaggg ctgcgtcccg aacgagatca tgggctgcgg catcgagccc
     1861 gagccgatgt gatcgctagc atcacgtagg tcgcgcagac cgacggccgc ctgcgggcgg
     1921 cgccgggccc gggcgatccc ctcgtgggat cgtccgggtc ttcgtcatgg cctgaagtgg
     1981 gccccctcgc ggatccgcgc aggggcaaac ttgttggatc gcgcgatcgc gctggtgctc
     2041 gcggtcgcgc tccgctaaag gaggagagga tgacacgatc ttctctttac ctactcctgg
     2101 gtgcgggcct ggccctcacc gccgcatgtg gcgacgataa cgctggcgag tccggcgcct
     2161 cggcgagcga gggcgcgacc gacagcgcga ccggcgtgac cggcgtgacc ggctcggcca
     2221 ccgacgccga cagcgcgacc gatcccacca ccgggatgtc ggcgtcgatg agctcgaccg
     2281 aggatccgac cggcgcgagc acgaccgacg tcaacgtgac cgagggcagc agcagcggcg
     2341 gcggcgacac ggaggcgagc acgaccggac ccgtcgaccc gggaacgacg accgacgtcg
     2401 agacgaccga cccgggcacg acgaccgacg ccgagacgag cacgaccggg cccgactcga
     2461 cgaccggcga ctcggaggag tgtccgctgt cgctgcagca catgccgtgc gacggcgcgt
     2521 cggacgacgc cctgcacgcg ctcgggctca actgcacgac cgcgggcaac cagtggatca
     2581 acaacgacaa cgcggtggcg gtcgccaagc tcgacttcca ggcgccgccg ccgtccggcg
     2641 gtcgtcgcac ctggcaggtc gccaagtcgt acggcacgca cgtcgacccg aacaccaaca
     2701 agccgttctg gggcccgcgc gagggcgaga aggtgctgct gatcagca
//
LOCUS       DASWNJ010000930         3375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_687231, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000930 DASWNJ010000000
VERSION     DASWNJ010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687231"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="VGB85_15755"
     CDS             complement(<1..1038)
                     /locus_tag="VGB85_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135540.1"
                     /translation="MRPALLLSLLLPACLADPPALVLDGAGCDEIDLAVLDPAVLPGW
                     TVHALVSESAEGDTAWTLATDPDGALLLKPWPEGPALDLSQLGEPGDFSLIRGRTDHE
                     SWLLLDRQDHLRVWRLGRAARGEVTPSPDLSGFPGPGGWHYSLLLVGDVPYLLAAATG
                     VQARMMRFQLAPLDPDHLALGPAGPALRFADLCMPLWEQPCSIFARATALAEVDELAT
                     TEAGAMPGAAVLLGVQFADGSPISSGFIQLQLDDRGPGFPPGVIGRMLALAGGQSPRL
                     HGRISSDGENLFTSVTSLDDDTAAPAPYFHIDFALADPTSLSQLGLSPYNGPILQFGR
                     EVVMTHLARGRW"
     gene            1425..2405
                     /locus_tag="VGB85_15760"
     CDS             1425..2405
                     /locus_tag="VGB85_15760"
                     /inference="COORDINATES: protein motif:HMM:NF039450.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADYC domain-containing protein"
                     /protein_id="HEY0135541.1"
                     /translation="MKRSSRYCSLVVPVVVLAGGCYANEEDDPGFNEFRSTGACTTCG
                     YGNGNSPYINRSDIPELHQFGLPNSTSVTIIGVRSPAHKLHHLRVIDDEFVALKGSTV
                     VASGGQLVGWTIILDTPDGQVDVHISAHDGQVPSLVDGGPMSSAYGLKYEHQDMPGEL
                     ISVCPGEGADDAVVTLVLGETYDPELKLVNADQAGWFTLACGGEAVAKMKLLNYGPSA
                     DFDGSGEPATVDQRQATLKMITADYCGTGTSYTVQGLAVVWADEAGTVGPTSPPAGAS
                     VEAIWTADGAVCLDTPRWADPQVVAEECVLPSCDQISPNTPGIVWRTYHP"
     gene            complement(2502..>3375)
                     /locus_tag="VGB85_15765"
     CDS             complement(2502..>3375)
                     /locus_tag="VGB85_15765"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY0135542.1"
                     /translation="AGKERVAGELHRLSGRRGPLVVLNCAALSPLLVESQLFGHVRGA
                     FTGATGEHPGLFRAAHGGTLFLDELGELPLDLQPKLLRAVEDGEVVAVGSTQRHRVDV
                     RLVAATNRSLCDEVEAGRFRRDLYARLALWELHVPPLRARRTDLLGWIDRLHHAWGAQ
                     RGHTTPAFEFETDALERLLLHRWPDNLRGLKRFVHERGAATGPVQLASLPAWLRPGSS
                     SSASASALPVPEDRSIRPQRPRPTREDLLAALEAHGWNVRATARHFERDRKQISRWIE
                     MYEVTIPTNIPGDD"
BASE COUNT          527 a         1095 c         1273 g          480 t
ORIGIN      
        1 ccagcggccg cgagcgaggt gggtcatcac gacctcgcgg ccgaactgca agatcgggcc
       61 gttgtacggc gagagcccca attgggacag gctggtcgga tcggcgagcg cgaagtcgat
      121 gtggaagtag ggggcaggcg cggcggtgtc gtcgtcgagg ctggtgaccg aggtgaacag
      181 gttctcaccg tcggaggaga tccggccgtg gaggcgcggg gactggccgc ccgcgagcgc
      241 caacattcga ccgatgacgc ccggcgggaa gccggggccg cggtcgtcga gctgcaactg
      301 gatgaagccg gaggagatcg ggctgccgtc ggcgaactgg acgccgagca gcacggcggc
      361 gccaggcatc gcgccggcct cggtcgtcgc cagctcgtcg acctcggcga gcgcggtggc
      421 ccgagcgaag atgctgcacg gctgctccca cagcggcatg cacaggtccg cgaagcgcag
      481 cgccgggcca gctggcccga gggccaggtg gtccgggtcg agcggcgcga gctggaagcg
      541 catcatcctg gcctgcacgc cggtcgcggc ggcgagcagg tacggcacgt cgccgacgag
      601 cagcaggctg tagtgccagc cgccagggcc cgggaagccg ctcaggtcgg gcgagggggt
      661 gacctctccg cgggctgcgc gaccgagccg ccacacgcgg aggtggtcct ggcggtcgag
      721 cagcagccag ctctcgtggt cggtgcggcc gcggatcagc gagaagtcgc cgggctcgcc
      781 gagctgcgag aggtcgagcg caggcccctc cggccacggc tttaatagga gggcgccgtc
      841 ggggtcggtg gcgagggtcc acgcggtgtc accctcggcg ctctcgctga cgagcgcgtg
      901 cacggtccag ccgggcaaga cggcggggtc gagcaccgcg aggtcgatct cgtcgcagcc
      961 cgcaccgtcg agcaccagcg ccggagggtc ggcgaggcag gcaggcagca gcagcgagag
     1021 caggagggca ggccgcacgg caccagcata cgccgggcgg cggccggacg cccgtttgcg
     1081 cccggagcga acgccgcgaa cgttccgcag tggcctggag ggcggcgacc aggtcctcgc
     1141 aggtcgggca gggacgctgc ggtcggaggg gcctgtcctg cgagacaggt gacgtggacg
     1201 cgactggccg cgttcgttgg gcgaagcgct cgaggccgcg caagtcgcaa gaggtcttga
     1261 aatcatctct caatcgcccg cgcgattcgt cgcctgggac atttgcacgg ccgacgagcc
     1321 ggtccacatt cgcaggttgc attgcgattg cagacatgtt ctaaaagccc acgctggcgc
     1381 tgtgcccggt cagcgaaatc gtccgttcca tcattgaggt atgtatgaag agatcatcgc
     1441 gctattgctc cctggttgtt cctgtcgtcg tgctcgccgg tggttgttac gccaacgagg
     1501 aggacgatcc cggtttcaat gagtttcgca gcaccggcgc ctgcaccacg tgcggctacg
     1561 gcaacggaaa ttccccatac atcaatcgct ccgatatccc cgagttgcac caattcggcc
     1621 tgcccaacag cacatcggtg accatcatcg gtgtccgcag cccggcgcac aagctccatc
     1681 atctgcgcgt gatcgacgac gagttcgtcg ccctcaaggg gtcgacggtc gtcgcgtcgg
     1741 gcggtcagct cgtcggctgg acgatcatcc tcgatacacc ggatggtcag gtcgacgtcc
     1801 atatctctgc ccatgacggg caggtcccga gcctggtgga cggcgggccg atgagctccg
     1861 cgtatggcct caagtacgag caccaggaca tgcctggcga gctgatcagc gtgtgtccgg
     1921 gcgagggagc ggacgacgcg gtcgtcacgc tggtgctcgg tgagacctat gaccccgagc
     1981 tcaagctcgt gaacgcggac caggccgggt ggttcaccct cgcttgcggc ggcgaggccg
     2041 tggcgaagat gaaactcctc aactacggcc caagcgcgga cttcgacggc agcggtgagc
     2101 cggccacggt ggatcagcgg caggcgacgc tcaagatgat caccgccgat tactgcggca
     2161 ccgggaccag ctataccgtc cagggtctcg cggtcgtgtg ggccgacgag gcaggcacgg
     2221 tcggtccgac ttcaccgccc gcgggcgcga gcgtcgaggc catctggacg gcggacggcg
     2281 ccgtgtgcct cgatacgccg cgctgggccg atccgcaggt cgtcgccgag gagtgcgtct
     2341 tgccgagctg cgaccagatc tcgccgaaca cccccggcat cgtctggcgg acttatcatc
     2401 cgtgagccgg cggaactgaa gcagagtcgc cgcccccatc acgggaaggc gagcactggc
     2461 atacgcccgg agcggcctgc gtccgtccgc gcggcgcgga gctaatcgtc gccggggatg
     2521 ttcgtcggga tcgtgacctc gtacatctcg atccagcgcg agatctgctt gcggtcgcgt
     2581 tcgaagtggc gggcggtcgc gcggacgttc cagccgtgag cctcgagcgc ggcgagcagg
     2641 tcctcgcggg tcgggcgggg ccgctggggt cggatggacc tgtcctccgg gacaggtaat
     2701 gcggacgcag acgccgagga tgacgagccg gggcgcagcc aggccggcag gctagcgagc
     2761 tgcaccgggc cggtcgcggc gccgcgctcg tggacgaagc gcttgaggcc gcgcaggttg
     2821 tcgggccagc ggtgcagcag caggcgctcg agcgcgtcgg tctcgaactc gaacgctggt
     2881 gtggtgtgcc cgcgctgcgc gccccaggcg tggtggaggc gatcgatcca gccgagcagg
     2941 tcggttcggc gcgcgcgcag gggcggcacg tgcagctccc acagcgcgag ccgggcgtag
     3001 aggtcgcggc ggaatcttcc cgcctcgacc tcgtcgcaca gcgagcggtt ggtcgcggcg
     3061 accaggcgca cgtcgacgcg gtgacgctgc gtgctgccga ccgcgacgac ctcgccgtcc
     3121 tcgaccgcgc gcagcagctt gggctggagg tcgagcggga gttcgccgag ctcgtcgagg
     3181 aacagcgtgc cgccgtgcgc ggcgcggaac aggcccgggt gctcgccggt cgccccggtg
     3241 aaggcgcccc gcacgtggcc gaacagctgg ctctcgacga gcagggggct gagcgcggcg
     3301 cagttgagga cgacgagcgg gccgcggcgg ccgctgagtc ggtggagctc gccggcgacg
     3361 cgctctttgc cggcc
//
LOCUS       DASWNJ010000931         3099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_652864, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000931 DASWNJ010000000
VERSION     DASWNJ010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652864"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VGB85_15770"
     CDS             <1..524
                     /locus_tag="VGB85_15770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135543.1"
                     /translation="AAAGMLVGGVGLPHRLVVFVEHDEHPNLGNLGPVLEYRRRHPGR
                     LAIQIIARGTTAAARQLRLRLCGAQKLGLELEYLRRLAQGPMTRGLVAADAVPLVERL
                     DEAAEAHACEMGEPELEPAPGGARGLPDGAWLDGTAVGQGDLEAIGVRIVGLEAAQRP
                     LDAVFSAAAPAEL"
     gene            596..2116
                     /locus_tag="VGB85_15775"
     CDS             596..2116
                     /locus_tag="VGB85_15775"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEY0135544.1"
                     /translation="MEIKHWAATDVGRKRSHNEDNFLIDKNLNLFVVADGMGGHASGE
                     VASAIAVHTIRDVVFAERDLLTQYTDFDATAQMELCTLLEYAVHSASARIFHKAQQEP
                     EKRGMGTTVVALLTIGQRGFIAYVGDSRIYLSRNGVVYPLTEDHSLMNELIRSGKVRA
                     DEFAVSPYANFKNAMTRAVGVYEHVEVDILDFEILPGDAFLLCSDGLYDYLDDAEIAS
                     ALALSNIEEIPGRFIDMANSRGGKDNITALVVQVAPDESSASRAAQLNFTLETLRRIP
                     LMDQLSYQQLSKIMNIARMQPCRVGELLCREGTPGGDLYAVLEGAVTLSRGGVPMTTI
                     HAGECFGETDLIAGETARATAIADKAGKLLKIGRRDVLEVLRRDPGLASKLLWNLARE
                     LGKRLEDAQLQLSSALGEDKTMVVDIDTLDTPDGVEETNPRAIVIPKLTTSSGPAPTS
                     ASPSASPMGSAKPVTSSVTAASSTAAAKSAAPKVNSGEPEILEEIDEFEDVLDEPG"
     gene            2103..2537
                     /gene="cdd"
                     /locus_tag="VGB85_15780"
     CDS             2103..2537
                     /gene="cdd"
                     /locus_tag="VGB85_15780"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEY0135545.1"
                     /translation="MNPDDPQAPQAQQDPQEAELLAAAWHAREAALCPYSKFAVGAAI
                     LDGEGRVWTGANVENASYTLGLCAERVALFYALTHGARDIRSVVVVTGAATPSSPCGA
                     CRQILFEFAAKATLVSATAGDQVLRIRVRDLLPHGFDASSLA"
     gene            2542..>3099
                     /locus_tag="VGB85_15785"
     CDS             2542..>3099
                     /locus_tag="VGB85_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135546.1"
                     /translation="MSLSGRASPDGTPSTPRRLALADAIAGLRGGGDGFAGLGELAGK
                     GEWKTPNSDISTPRADDVDTIEDGAPPRSPSPLTPGADEAARFPITTRGTYEIAGKFA
                     QGGIGRILRAHDPVLGRTVALKELLVAGHRTDEERFVREVLLTARLQHPGIVPVYAAG
                     RWPSGEPFYAMKLVSGRSFDQVIDDA"
BASE COUNT          483 a         1163 c         1043 g          410 t
ORIGIN      
        1 cggccgcggc cggcatgctg gtcggcggcg tcggcctgcc gcaccgcctc gtcgtcttcg
       61 tcgagcacga cgagcacccc aacctcggca acctcggtcc cgtgctcgag taccgccgcc
      121 ggcaccccgg ccgcctggcg atccagatca tcgcccgtgg caccacggcg gcggcccgcc
      181 agctgcgcct gcgcctgtgc ggggcgcaaa agctgggcct ggagctggag tacctgcgcc
      241 gtctcgcgca gggcccgatg acccggggcc tggtggccgc ggacgcagtt ccgttggtag
      301 aacgcctcga cgaggccgcc gaggcgcacg cctgcgagat gggcgagccc gagctcgagc
      361 cggcgccggg cggcgcgcgc ggcctgccgg acggcgcctg gctggacggt accgccgtcg
      421 gccagggaga tctcgaggcg atcggcgtgc ggatcgtcgg cttggaggcc gcccagcggc
      481 cgctcgatgc cgtattctcg gcggcagcgc ccgcggagct gtgacaccca gtcctcgaga
      541 gcacgcgcca ccgtagagcc caccgagagt catacccgaa gcaggtcgca gcacgatgga
      601 gatcaagcac tgggcagcca ccgatgtggg acgcaagcgg tcccacaacg aggacaactt
      661 cctcatcgac aagaacctga acctcttcgt cgtcgccgat gggatgggcg gccacgcgtc
      721 gggagaggtg gccagcgcga tcgcggtgca cacgatccgc gacgtcgtgt tcgccgagcg
      781 tgacctgctg acgcagtaca ccgacttcga cgcgacggcg cagatggagc tgtgcacgct
      841 gctcgagtac gcggtccaca gcgcctcggc ccgcatcttc cacaaggccc agcaagagcc
      901 cgaaaagcgc ggcatgggca cgaccgtggt cgccctgctg acgatcggtc aacgcgggtt
      961 tatcgcctac gtcggcgaca gccggatcta cctcagccgc aacggcgtcg tctacccgct
     1021 gaccgaggac cacagcctga tgaacgagct gatccgcagc ggcaaggtcc gcgcggacga
     1081 gttcgcggtg tcgccctacg cgaacttcaa gaacgcgatg acgcgggcgg tcggcgtcta
     1141 cgagcacgtc gaggtcgaca tcctcgactt cgagatcctg cccggtgacg cgttcctgct
     1201 ctgctcggac ggcctctacg actacctcga cgacgccgag atcgcgagcg ccctggcgct
     1261 gtcgaacatc gaggagatcc ccggccgctt catcgacatg gccaacagcc gcggcggcaa
     1321 ggacaacatc accgcgctgg tcgtgcaggt cgcccccgac gagagctcgg ccagtcgcgc
     1381 ggcgcagctc aacttcaccc tcgagaccct gcgccggatc ccgctgatgg accagctgtc
     1441 gtaccagcag ctgtccaaga tcatgaacat cgcccgcatg cagccgtgcc gcgtcggcga
     1501 gctgctgtgc cgcgagggca cccccggcgg cgacctctac gccgtgctcg agggcgccgt
     1561 cacgctgtcg cgcggcggcg tgccgatgac gacgatccac gccggcgagt gcttcggcga
     1621 gaccgacctg atcgccggcg agaccgcccg cgcgaccgcg atcgccgaca aggccggcaa
     1681 gctgctcaag atcggccgcc gcgacgtgct agaggtcctg cgccgggatc ccggcctcgc
     1741 ctcgaagctg ctgtggaacc tcgcccgcga gctcggcaag cgcctcgagg acgcccagct
     1801 ccagctgagc agcgcgctcg gcgaggacaa gacgatggtc gtcgacatcg acacgctcga
     1861 cacccccgac ggcgtcgagg agaccaaccc gcgcgcgatc gtcatcccca agctgacgac
     1921 ctcgtccggg cccgcgccga cctcggcctc gccctccgcc tcgccgatgg gctccgcgaa
     1981 gcccgtgacc tcctcggtga ccgcggcatc ctcgaccgcg gcagcgaaga gcgccgcgcc
     2041 gaaggtgaac agcggcgaac ccgagatcct cgaggagatc gacgagttcg aggacgtgct
     2101 cgatgaaccc ggatgacccg caagccccgc aagcccagca ggacccgcag gaggccgaat
     2161 tgctggccgc cgcatggcac gcccgcgagg ccgcgctgtg tccgtacagc aagttcgccg
     2221 tcggcgcggc gatcctcgac ggcgagggtc gcgtgtggac cggcgccaac gtcgagaacg
     2281 ccagctacac gctcggcctg tgcgccgagc gggtcgccct gttctacgcg ctgacccacg
     2341 gcgcccgcga catccgcagc gtcgtcgtcg tcaccggcgc ggccacgccg tcgagcccgt
     2401 gcggggcttg ccgccagatc ctcttcgagt tcgccgccaa ggccaccctg gtctcggcga
     2461 ccgccggcga ccaggtcctg cgcatccgcg tccgggacct cctaccccac ggcttcgacg
     2521 cctcgtcgct cgcgtgaaag catgtcgctc tccggtcgcg cctcgcccga cggcaccccg
     2581 tcgaccccgc ggcggctcgc gctcgcggac gcgatcgccg ggctgcgcgg cggcggtgac
     2641 ggttttgccg ggctcggcga gctcgctggc aagggcgagt ggaagacgcc caacagcgac
     2701 atcagcacgc cgcgggccga cgatgtcgac acgatcgagg acggcgcgcc gccccgcagc
     2761 ccgtcgccgc tgaccccggg cgccgacgag gcggcccgct ttccgatcac cacccgcggc
     2821 acctacgaga tcgccggcaa gttcgcccag ggtggcatcg ggcggatcct tcgcgcccac
     2881 gacccggtgc tcggccgcac cgtcgcgctc aaggagctgc tggtcgccgg gcaccgcacc
     2941 gatgaggagc gcttcgtccg cgaggtgctg ctgacggccc gcttgcagca cccgggcatc
     3001 gtcccggtct acgccgccgg ccgctggccc tcgggcgagc cgttctacgc gatgaagctg
     3061 gtctcgggcc gctccttcga tcaggtgatc gacgacgcc
//
LOCUS       DASWNJ010000932         1796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_328654, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000932 DASWNJ010000000
VERSION     DASWNJ010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328654"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..718)
                     /locus_tag="VGB85_15790"
     CDS             complement(92..718)
                     /locus_tag="VGB85_15790"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0135547.1"
                     /translation="MSKQTKIYWIKAQAPRRVLALAKHLKIDAEFIEVDMMAGGLKTP
                     DYAALNPNMKAPTLVDGDFVLWESSAIMAHLCIAAGSDMWPAHAPAEQVEVLRWLSWN
                     DQHWAEAVGPFYFEHIVKTTFGFAEPDPAALVDKVDGLLRVARVLDGHLQGREFVACG
                     RLTIADFQLASMATDWREAEMPFGDFKNIRRWIEGLMRIPAWADPWPR"
     gene            803..1438
                     /locus_tag="VGB85_15795"
     CDS             803..1438
                     /locus_tag="VGB85_15795"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0135548.1"
                     /translation="MPYTAEHKARTRERIVLAARELFNRHGFESVSIDQIMARAELTR
                     GGFYNHFKSKDALYAEAVRSFITCNPFAMQLARSRRPMPGPRKLARMLVEMYLSDEIL
                     EDADRQCPLYALPSDCARAGLEPQKAYTDLIRSLTTVFKNAIPEDAADADERVHLIVT
                     LCVGGMVLARTTHDPGLRETLRATARKQALALLGHDDAAPSPRTTRRDAAS"
     gene            complement(1485..>1796)
                     /locus_tag="VGB85_15800"
     CDS             complement(1485..>1796)
                     /locus_tag="VGB85_15800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135549.1"
                     /translation="FKAKGLKHRTAGTRTAVQVKAEVVGSVAEFVALMVGEARLQQGL
                     AAVCGGALEAKHTGKFLAWVAADVRKESVAELEAAGLTWAQVEKAVQTRAREWFLAQR
                     A"
BASE COUNT          282 a          689 c          551 g          274 t
ORIGIN      
        1 gcgcggccag atcaccgagc tcagcgtcgg tgcgaccggc gttgagccgc cgcccgagct
       61 atccgagccc gccgcagctc ccggctgacg ctcagcgcgg ccaggggtcc gcccacgccg
      121 ggatccgcat cagcccctcg atccaccgcc ggatattctt aaaatcaccg aacggcatct
      181 cggcctcgcg ccagtcggtc gccatcgagg cgagctggaa gtccgcgatc gtcaggcgcc
      241 cgcaggcgac gaactctcgc ccctgcaaat gtccgtcgag caccctggcg acgcgcagca
      301 gcccgtccac cttgtccacg agcgccgcgg gatcgggctc ggcgaagccg aaggtcgtct
      361 tgacgatgtg ctcgaagtag aacggcccga ccgcctcggc ccagtgctgg tcgttccagc
      421 tcagccagcg cagcacctcg acctgctccg cgggcgcgtg cgcgggccac atgtcggacc
      481 cggccgcgat gcacaggtgg gccatgatcg ccgacgactc ccacagcacg aagtcgccgt
      541 cgaccagcgt cggcgccttc atgttgggat tgagggccgc gtagtccggg gtcttgaggc
      601 cgcccgccat catgtcgacc tcgatgaatt cggcgtcgat cttgaggtgc ttcgccagcg
      661 ccagcacccg ccgcggcgcc tgcgccttga tccagtagat cttcgtttgt ttgctcatgg
      721 gcggcaacat agcggccgtc ttcttatgtt caagaacata ttgtgcgatc ctgctatgtg
      781 taccaaagga ggcgccccgc cgatgcccta caccgccgaa cacaaggctc gcacgcgcga
      841 gcgcatcgtg ctcgccgcgc gcgagctgtt caatcgtcac ggcttcgaga gcgtctcgat
      901 cgaccagatc atggcgcgcg ccgagctgac gcgcggcggc ttttataatc attttaagag
      961 caaggacgcc ctctacgccg aggccgtgcg cagcttcatc acctgcaacc ccttcgccat
     1021 gcagctggcc cgctcgcggc ggccgatgcc ggggccacga aagctcgcgc gcatgctcgt
     1081 cgagatgtac ctcagcgacg agatcctcga ggacgccgac cgccagtgtc ccctgtatgc
     1141 cctgccctcg gactgcgccc gcgccggcct cgagccgcag aaggcctaca ccgacctgat
     1201 ccgcagcctg acgaccgtct tcaaaaatgc gatccccgag gacgccgcgg acgccgacga
     1261 gcgcgtgcac ctgatcgtca cgctgtgcgt cggcggcatg gtgctcgccc gcacgaccca
     1321 cgaccccggc ctgcgcgaga ccctgcgcgc gacggcccgc aagcaggcgc tcgcgctgct
     1381 ggggcacgac gacgcggcac cgtcgcctcg caccacccgg cgtgacgccg cctcgtgatc
     1441 cacgaatgcg cggcgcgccg gcatcaccac cgcgcgccgc cacttcacgc ccgctgcgcg
     1501 aggaaccact cgcgcgcgcg cgtctgcacg gccttctcga cctgggccca cgtcaggcct
     1561 gcggcctcga gctcggcgac cgactccttg cggacatcgg cggcgaccca cgccaggaac
     1621 ttgcccgtgt gcttcgcctc gagggccccg ccgcacacgg cggccagccc ctgttgcagg
     1681 cgcgcctcgc cgaccatcag cgcgacgaat tccgcgaccg accccaccac ctcggccttc
     1741 acctgcacgg ccgtcctcgt gcccgcggtg cgatgcttga ggcccttggc cttgaa
//
LOCUS       DASWNJ010000933         2084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_52460, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000933 DASWNJ010000000
VERSION     DASWNJ010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52460"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1329
                     /gene="tssC"
                     /locus_tag="VGB85_15805"
     CDS             <1..1329
                     /gene="tssC"
                     /locus_tag="VGB85_15805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HEY0135550.1"
                     /translation="IAEIDRRLSAQVSAILHHEKVQRLEAAWRGVKYLVDATDFGQNV
                     RLELQSVTKEELKDDFEDAPDVTQSGLYKTVYRDSFGTLGGTPYGVICSTYDFGPGGE
                     DVALLKNCAAVASMAHAPFLANAAPRLLGLDSFVDLPKIKNVSAIFDGPQTSRWDALR
                     DTEEARFLGICLPRFLLRGPYGEPVHVPDTSSEYVPETQRGETPTVRAFAYTEDVVDH
                     HERYLWGPASLALAARIADSFARYGWGPNIIGPQGGGSIHHLPLHIYEQSGELKTKHP
                     VEAAIDDGTEKALSECGLIGLQFRKHSADAVFMSARSLRRPQMFPRSPEGQAQQRSAD
                     IGAQLPFVFVISRIAHYMKVLQREQIGSWKTRSDVERELNQWLTGFVSDMPDPSAETR
                     ARKPLRRARVTVEEIPGQQKWFRCALAVEPHFKLEGVEVSLGLVGKLDRV"
     gene            1342..>2084
                     /locus_tag="VGB85_15810"
     CDS             1342..>2084
                     /locus_tag="VGB85_15810"
                     /inference="COORDINATES: protein motif:HMM:NF017739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HEY0135551.1"
                     /translation="MPQNLAPPRYVFTCSDLGSATVHVRRFKLVEALSTPYRLTIELI
                     TPDLETDVAGLLGASCELRIDRVGTARSVAGVVERARQLNVVANRLRFSLEIVPAFAL
                     LEQVVNTRFFQNLDAAQIIEQVLRPGLAASGRSLRMELSATVPPREYCVQYRESDYAF
                     ASRLMQEEGITYRFEHSTGGPEFLVLMSETRQCPDLDRADGTSLTFIDRATASATVES
                     FDTFIPIYALQTTSIVQADFDWMAPPSAYL"
BASE COUNT          321 a          795 c          654 g          314 t
ORIGIN      
        1 atcgccgaga tcgaccgccg gctctcggcc caggtcagcg cgatcctcca ccacgagaag
       61 gtccagcgcc tcgaggccgc gtggcgcggc gtcaagtacc tcgtcgacgc caccgacttc
      121 gggcaaaacg tgcggctcga gctccagtcg gtcaccaagg aggagctcaa ggacgacttc
      181 gaggacgccc cggacgtcac gcagtccggg ctctacaaga ccgtctatcg cgacagcttc
      241 gggaccctcg gcggcacgcc ctacggcgtg atctgctcga cctacgactt cgggcccggc
      301 ggcgaggacg tcgccctgct caagaactgc gccgcggtcg cctcgatggc ccacgcgccg
      361 ttcctcgcca acgccgcccc gcggctgctc ggcctcgact cgttcgtcga cctgccgaag
      421 atcaagaacg tctcggcgat cttcgacggc ccgcagacct cgcgctggga cgccctgcgc
      481 gacaccgagg aggcgcgctt cctcggcatc tgcctgccgc gcttcctgct gcgcggaccc
      541 tacggcgagc cggtccacgt cccggacacc tcgtcggagt acgtgcccga gacccagcgc
      601 ggcgagacgc cgacggtgcg cgccttcgcg tacaccgagg acgtcgtcga ccaccacgag
      661 cgttatctct ggggtccggc ctcgctggcg ctggcggccc ggatcgccga cagcttcgcc
      721 cgctacgggt gggggccgaa catcatcgga ccgcagggcg gcggatcgat ccaccacctg
      781 ccgctccaca tctacgagca atccggcgag ctcaagacca agcacccggt cgaggccgcg
      841 atcgacgacg gcaccgagaa ggcgctctcc gagtgcggcc tgatcggcct gcagtttcgc
      901 aagcacagcg ccgacgcggt gttcatgtcg gcgaggtccc tgcgccggcc gcagatgttc
      961 ccgcgctccc ccgagggcca ggcccagcag cgcagcgccg acatcggcgc ccagctgccc
     1021 ttcgtgttcg tgatctcgcg gatcgcccac tacatgaagg tgctgcagcg cgagcagatc
     1081 gggtcgtgga agacccgctc cgacgtcgag cgcgagctca accagtggct gaccggcttc
     1141 gtgtccgaca tgccggatcc atcggcggag acgcgcgccc gtaaaccact gcgcagggcc
     1201 agggtcacgg tcgaggagat ccccggacag cagaagtggt tccgctgcgc gctggcggtc
     1261 gagccccact tcaagctcga gggcgtcgag gtgagcctcg gcctcgtcgg caagctcgac
     1321 cgcgtatagg gggccccgtc catgccgcaa aacctggcgc ctccgcgcta tgtcttcacg
     1381 tgcagcgacc tcgggtcggc gaccgtccat gtccgccgct tcaagctcgt cgaggcgctg
     1441 tcgaccccgt atcgcctgac gatcgagctg atcacgccgg acctcgagac cgacgtcgcg
     1501 ggcctcctgg gcgcctcctg tgagctgcgc atcgaccgtg tcgggaccgc ccgctcggtc
     1561 gccggcgtcg tcgagcgcgc gcgccagctc aacgtcgtcg ccaaccgcct gcgcttctcc
     1621 ctcgagatcg tgccggcgtt cgccctgctc gagcaggtcg tcaacacccg cttctttcag
     1681 aacctggacg ccgcgcagat catcgagcag gtgctgaggc ccggcctcgc cgccagcggc
     1741 cgatcgctgc gtatggagct cagcgccacc gtgccccccc gcgagtactg cgtccagtac
     1801 cgcgagtccg actacgcgtt cgcgtcgcgg ctgatgcagg aggagggcat cacctaccgc
     1861 ttcgagcact cgaccggcgg ccccgagttc ctcgtgctga tgagcgagac ccgccagtgc
     1921 cccgacctcg accgcgccga cggcacctcc ctcaccttca tcgaccgggc caccgccagc
     1981 gccaccgtcg agtcgttcga caccttcatc ccgatctacg ccctgcagac cacctcgatc
     2041 gtccaggccg acttcgactg gatggccccg ccgtcggcgt acct
//
LOCUS       DASWNJ010000934         5167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_194269, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000934 DASWNJ010000000
VERSION     DASWNJ010000934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194269"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VGB85_15815"
     CDS             complement(<1..644)
                     /locus_tag="VGB85_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135552.1"
                     /translation="MRHIEFDKIRGDTSEGRRARFEQLVCHLARLDKVVAGDFRRIDG
                     SGGDGGVEAIWRLLSGGVVGYQAKYHLHRDGIDWAKIDRSVQTALSHYPNLERYVIAL
                     PCDLTGRRAVRGGSTEGAWGIWDTHLAEWRRSAASRGMTVAFEHWTASELEERLAREE
                     AHHLRRYFFDELVLSRAWIDRHLNRTLIDLHTRYSPDEHVPTSSLMVFDVLFRR"
     gene            1991..2239
                     /locus_tag="VGB85_15820"
     CDS             1991..2239
                     /locus_tag="VGB85_15820"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0135553.1"
                     /translation="MPIQIEGVEYFTAADIQRSIGVVRQTLWRWRRARKIPQGRRYRD
                     KSIVFTREEVEAIREFSNRLEPADSALPRRPSRPGDSA"
     gene            2285..3835
                     /locus_tag="VGB85_15825"
     CDS             2285..3835
                     /locus_tag="VGB85_15825"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0135554.1"
                     /translation="MKADHKEKREDCSIIALDKFIQATRDSGYKGTASAISELVDNSL
                     QAGATRIAIHVEAKTSDEEKAIEVSVLDNGCGMDPATLRQALRFGGSTRFGDRRGLGR
                     YGMGLPNASLSQARRVTVYTWQSTRVYSCYLDVDEIVRCEMIEVPAPEIITAPPSACA
                     GTSGTLVSWSQCDRLDNRRVSTIVRKFEAELGRRFRHFIWDGVQITINGDTLEAFDPL
                     YLNPKAEVSGAKLFGEEMRFEVRADAGDAQKTGWVRVRFTELPVHAWHKLSNDEKRRI
                     GLSKGAGVSIVRGGREVDYGWFFMGTKHRENYDDWWRCEIQFDPILDEAFGITHTKQQ
                     ARPQAHLLEALTPDLEATARALSGRARKAHLAVKTSARVSEAERIANERDHLLRPLPR
                     SADPMARALMRELEASHPALKERGDGHDRYSIIEHAVKDTSFFTMAHDGDRLVLVLNP
                     DHPFYREIYKPLSEGETSRDPQLRAKLELLLLAAARSEAAARGKVPALAKHRLEWSNT
                     LAAFLNDK"
     gene            3841..>5167
                     /locus_tag="VGB85_15830"
     CDS             3841..>5167
                     /locus_tag="VGB85_15830"
                     /inference="COORDINATES: protein motif:HMM:NF025596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Druantia anti-phage system protein DruA"
                     /protein_id="HEY0135555.1"
                     /translation="MPDSPWTPLEPPLSGKASLDVFVEHARALVKVRSDERTDEAIEE
                     IRAEFVAAVKASRVVDRQALLAAGLVVTDLALQGWVLRVRTGRVEIRPPDAVSGDRAA
                     EKARVRKQELVKRNAQLRQPSVQRFLESMELQRLHNGEFVSIYSLMRDGREFAAGLRE
                     ARLHLSNGWADALSKLVDPYLEFVTSGDAACQHTGLRLMDVWRYFRHTWTNQYTQAPG
                     RTMAFLVRDRAAANHPVMGIGALSSPIMQIRERDTWIGWHPETFLARVRREPTGKLAR
                     WLVETLDAALNEIYVEDLLEDEVLSTRDFYAPSDAALKRLQREAVEQRRLHHRYARSR
                     DLKQKHAEASGDDYWVARARTHLFRSKRALALATFLRARAVLNEAFNGKPTAKKLAAL
                     ATTGYGSDAIRRVLKKARADRVGIAVADISVCGAVQPYNALLGGKLVAML"
BASE COUNT          969 a         1637 c         1712 g          849 t
ORIGIN      
        1 tcgcggcgga atagcacatc gaaaaccatt aggctgctcg tgggaacgtg ctcgtcagga
       61 ctatagcgag tgtggaggtc gatgagcgtc cgattcaggt ggcggtctat ccacgcgcga
      121 gacaggacga gctcgtcgaa aaagtaccgg cgaaggtggt gcgcctcctc ccgcgccagc
      181 cgctcctcga gctcagaagc ggtccagtgt tcaaacgcca ccgtcatccc gcgcgacgcg
      241 gccgaccttc gccactctgc aagatgtgtg tcccaaattc cccaggcgcc ttcagtgctc
      301 ccgccccgca ctgcccttcg tcctgttagg tcacaaggga gggctataac gtagcgctcc
      361 aagttcgggt agtgggacag cgccgtctgc acggacctat cgatcttcgc ccagtcgatc
      421 ccgtcacggt gaagatggta cttcgcttga taaccgacta cgccgccgct caggagccgc
      481 cagatcgcct cgacgccgcc gtcaccaccc gatccgtcga tgcgccgaaa atcgccagcc
      541 acaaccttgt cgaggcgcgc cagatgacac acgagttgct caaagcgggc ccgccggccc
      601 tcgctcgtgt caccgcggat cttgtcgaac tcgatgtgtc gcatgctatg gcgacggtac
      661 caccatcgac agggtgggat cctctcgggt ggacaagcgg aggccggtgt ctatgggtca
      721 tgcgcggacg acgcggtgac aaatcccaag caatttcgaa tatttgcggg atgggcttga
      781 ccgcgcctcg gtgtccgggt gtgatccgct cacaatgagc gctcagcccc aagatccctc
      841 cctctggttc tttcaagagt caatcactgg ccctgccgtc gtgacgtgct tgacgcacgg
      901 gacttggatt ctcgcaactg aggacggaga aaatgcagct cagttcgatg gccgtaacgt
      961 caacctcgcc gcgctttatg ttcaccacga tttcatcctt aggcccgaag taaaaggcaa
     1021 aggcttcgtt tcgggatatc gcgtgttgtg agcgcgtcga cggcgagtgg cctgtcgtgc
     1081 aaggactacg cggcgaaggc caaggtcaag ctccccccag ccgcggttat agccgcaccg
     1141 ctcgatgatg gggcggcggg tgcccgatcc attgagccgc gcccacgggc tctcgggcta
     1201 acccgggttt ccaaagccgg cggtgacgcg gcctgctcct gatcgcccgg tcgccgcagc
     1261 tcgtcggccc tcacccatcg acctgtgcct gctgcggcgc cagcctgcag gccctggcgc
     1321 aggcattgcc gaaccggcgg cgctggcggt cgcaccggat gggtcgaaca tggcctcgaa
     1381 agcgctggtg ggatgggcgg gcgggggtta ggcggactgg cgcacgcgac gcgggccacg
     1441 gccctcatgt cccgccctgc tagaccagcc atggattaag cctccgacag tgaagtggaa
     1501 ctcggcgatc cgctgcgaac gtgatcgccg cgatctgccg cgaccgcgtg gattccgcgc
     1561 tcctaagccg cccccgccgg cccagcgggg gccagacgct gcgggcctgt gccggtgaat
     1621 gtggtaaggg ggcgtcgcgt tggtcctggt agcggagcaa actgcgctca cgctcgccca
     1681 gagtagcgac gggcgccgca gaactcggcc tgatactgcg gttttggtcg ccggacgagg
     1741 cggcacgagc ggctaggcct gtcgtcctgc ttgcggatgg gcagtgctcg ccgcgagtta
     1801 ccaccagaag aagattctac gtctctccgg tggcgctcac gtcgagattc agcgcgccga
     1861 atgggcagac gagtagccgg gccagcagtg gtcatgccct gttgactcgc tacgggagcg
     1921 atagtatgca cccgccaggg acaagcgaga cgaatggtta cgatgtgcaa cctgaacaca
     1981 tgagcaggaa atgccgatcc agatcgaggg agtcgagtac tttaccgctg cggatatcca
     2041 gcggtcgatc ggcgtcgtgc gacagaccct gtggcgttgg cgccgggctc ggaagatccc
     2101 ccaggggcgc cgctatcgcg acaaaagcat cgtcttcacg cgcgaggagg tggaggccat
     2161 tcgcgagttc tcaaaccgtc tcgagcccgc ggactcggct ctgccccgac gacccagccg
     2221 cccaggcgac agtgcgtgac gaggaggatg tgtgatgcca ggcataaatc cacaacagcg
     2281 agtagtgaag gcagaccaca aagagaaacg agaggactgc tcgatcatcg cgttggataa
     2341 gttcatccaa gcgacgcgcg actcgggcta caaggggaca gccagcgcga tctcggaact
     2401 cgtcgacaac tcgctccagg ccggcgcgac gcgcatagcg atacacgtag aggctaagac
     2461 gagtgacgag gagaaagcga tcgaggtgtc ggtgctcgac aacggctgcg ggatggaccc
     2521 agccacgctg cgccaagcgc tccgcttcgg cggcagcacg cgcttcggag atcgccgagg
     2581 cctcgggcgc tacggcatgg gcctcccgaa cgcgtcgctg agccaggcgc gtcgggtcac
     2641 cgtctacacc tggcagtcaa ctcgcgtgta ctcctgctac ctcgacgttg acgagatcgt
     2701 acgttgcgag atgatcgagg tgcctgcgcc cgagattata acggcccctc cgagcgcgtg
     2761 cgctgggacc tccgggaccc tcgtgagctg gtcacagtgc gaccgactcg acaaccgacg
     2821 cgtctcgacg atcgtgcgga agttcgaggc tgagttggga cggcggttca ggcacttcat
     2881 ctgggatggg gttcagatca cgatcaacgg tgacacgctc gaagccttcg acccgctcta
     2941 cctgaacccg aaggcggagg tctctggcgc gaagctcttc ggcgaggaga tgcgcttcga
     3001 ggtccgcgca gacgcaggcg acgcgcaaaa gacaggctgg gtgcgggtgc ggttcacaga
     3061 actgccggtc cacgcgtggc acaagctctc gaacgatgag aagcgccgca tcggcctctc
     3121 caagggcgcc ggggtctcca tcgtacgagg gggccgcgag gtggattacg gctggttctt
     3181 catggggacc aagcaccgcg agaattacga cgactggtgg cggtgcgaga tccagttcga
     3241 cccgatcctc gacgaggcgt tcggcatcac gcacacgaag cagcaggctc gaccgcaggc
     3301 gcacctgctc gaggcgctaa cgccggacct cgaggcgacc gcccgcgcgc tgagcggccg
     3361 ggcccgcaag gcacacctcg ccgtcaagac gagtgcacgt gtctctgagg cggagcggat
     3421 cgccaacgag cgagatcacc ttctccgccc gttgccgcgc agcgcggacc ccatggccag
     3481 agcgctgatg cgagagctgg aggcgagtca ccctgcgcta aaggaacgcg gagatgggca
     3541 cgatcggtac agcatcatcg agcacgcggt gaaggacacg tcgttcttca cgatggcaca
     3601 cgacggggac cgtctcgtgc tcgtgctcaa ccccgatcat cccttctatc gagagatcta
     3661 caagcccctc tctgaggggg agacctcgcg cgatccacaa ctccgcgcga agctcgaact
     3721 cctgctcctc gcggccgcgc ggagcgaagc ggctgcgcgc ggcaaggtgc ccgcgctagc
     3781 gaagcaccgg ctcgagtgga gtaacaccct ggccgcgttc ctgaacgaca agtgagccac
     3841 gtgcccgata gcccctggac tcccctagag ccgccactct cgggcaaggc gtcgctagac
     3901 gtcttcgtgg agcacgcgcg cgcgctcgtg aaggtgcgta gcgatgagcg caccgacgag
     3961 gccatagagg agatccgcgc ggagttcgtc gcggcagtga aggcgtcgcg cgtcgttgac
     4021 cgtcaagcgc tcctcgcggc cgggctcgta gtcaccgacc tcgcgttgca ggggtgggtg
     4081 ctccgcgtgc ggactggtcg cgtcgaaatc aggcccccgg acgccgtcag cggcgacagg
     4141 gccgcagaga aggcacgcgt ccgtaaacaa gagctggtga agcgaaacgc gcagttgcgt
     4201 cagccctcag tccagcggtt cctcgagtcg atggagttgc aacggctcca caacggcgaa
     4261 ttcgtctcga tctattcgtt gatgcgcgac gggcgcgagt tcgcagccgg gctgcgagag
     4321 gcgcgtttgc atctcagtaa cgggtgggcc gacgccctct ctaaactggt cgacccgtac
     4381 ttggagttcg tcacctcagg ggacgccgcg tgccagcaca ctgggctgcg tctcatggac
     4441 gtctggcgtt atttccgcca tacgtggacg aaccagtata cgcaggcgcc gggacggacg
     4501 atggcattcc tcgtccgcga ccgcgccgca gcgaaccacc cggtgatggg gatcggcgct
     4561 ctgagcagtc cgatcatgca gatccgcgag cgggacacct ggatcggctg gcacccggag
     4621 acgttcctcg cgcgagtacg ccgcgagccg acaggcaaac tcgcgcggtg gctcgtggag
     4681 actctcgacg cggcgctgaa cgagatctac gtcgaggatc tactcgagga cgaggtcctc
     4741 tcgacgagag acttctacgc gccctccgat gctgcgctca agcggctgca gagggaggct
     4801 gtggagcagc gacgtctgca ccaccgctac gcgcggtcgc gcgacctgaa gcagaagcac
     4861 gcggaagcgt ccggcgacga ttactgggtc gcgcgcgcgc ggacgcacct gttccgcagc
     4921 aagcgcgcgc tcgcactagc gacgttccta cgcgcgcgcg cggtgttgaa cgaggcgttc
     4981 aacggaaagc cgacggccaa aaagctcgcc gcgttggcga cgacgggcta cggcagcgac
     5041 gcgattcgcc gggtgctgaa gaaggcgagg gccgatcgtg ttggtatcgc ggtcgcggat
     5101 atatcggtgt gcggcgccgt acagccgtac aacgcattgc tcggcggtaa gcttgtggcg
     5161 atgttgg
//
LOCUS       DASWNJ010000935         3996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_475867, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000935 DASWNJ010000000
VERSION     DASWNJ010000935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475867"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1505)
                     /locus_tag="VGB85_15835"
     CDS             complement(<1..1505)
                     /locus_tag="VGB85_15835"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0135556.1"
                     /translation="MQEDTDTVTPASGFDGLELPEALLRVLREVGYETPSPIQAQTIP
                     HLMAGRDLLGQAQTGTGKTAAFALPILARLELDKRPPQALVLTPTRELGIQVAEAFQR
                     YATHLPGFRVLPIYGGQSYGPQFQALSRGVHVVVGTPGRIIDHLERGSLDLSQLKYLV
                     LDEADEMLRMGFIDDVETILRKTPAERQTCLFSATLPAPIRRIAQTYMRKPVEVTIAA
                     KTRTAENIRQRFWQVSGMHKLDALTRILEAEPFDAMIVFARTKQSTDELAERLRARGF
                     AAEAINGDIQQSQRERTIAMLRDGKIDILVATDVAARGLDVDRVSHVINFDVPTDPES
                     YVHRIGRTGRAGRSGEAILFISPREQNLLRIIERATRQPITLMQLPTIKAVNDVRIGK
                     FKQQIRDTLASGDLDGYRGIIEEFEREENVPAIEVAAALARLFRGDEPLLLVKPDRSN
                     ERPPERADDRRPERSFEPRPEGGERPKKQRQERAPDPGMATFRVEVGYQHN"
     gene            1725..2033
                     /locus_tag="VGB85_15840"
     CDS             1725..2033
                     /locus_tag="VGB85_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135557.1"
                     /translation="MEQNITTHTFKRDDTWETIDTLWYSPFFYWQKNGLQVTPKVPLR
                     IITFFGTLIDESNEGWINTGGPSAILLQKIQARGVRGQTIRVETGEEIVEAPETSARS
                     "
     gene            2188..>3996
                     /gene="glgX"
                     /locus_tag="VGB85_15845"
     CDS             2188..>3996
                     /gene="glgX"
                     /locus_tag="VGB85_15845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEY0135558.1"
                     /translation="MPLHRRRGNAYPLGATWDGHGVNFAIYSENATSVELCLIDEEGI
                     ETRVRLRDPTAFVWHTYLEGIKPGQRYGYRVYGPWEPERGLRFNPRNLLTDPYAMAFS
                     GVTVWEDGAFSYDLEDPSDDRDLTINPRESLGVPLSVVVDRSFNWGDDQSPNVPLHGL
                     IIYETHIKGLTMRHPDVPEELRGTYAAVASDPIIKHLKELGVNAIELLPVHQFVDDKH
                     LLDRGVRNYWGYNSIGFFAPDVRYRCGDEITGEVRQFKEMVRRLHQAGIEVILDVVYN
                     HTAEGNHYGPTFSFKGVDNPTYYRLVPDNPRFYYDYTGTGNSLNVRHPQTLQLIMDSL
                     RYWVTEMHVDGFRFDLAAALARGLYEVEQLSSFFTIIHQDPIISQVKLIAEPWDVGPG
                     GYQVGNFPVRWAEWNGKYRDTMRAFWRGEGGKAGEVGYRLSGSSDLYQNDGRRPYSSI
                     NFITAHDGFTLADLVSYNDKHNEANGEGGNDGDSHNLSWNCGVEGPTDDPEVLALRRR
                     QQRNLMATLLLSQGTPMISGGDEIGRTQRGNNNAYCQDNEISWYDWDLDDDRKAMLAF
                     TQRVIGIYRKHPALQRAKFFKGRKIRGTDVRDIMWFN"
BASE COUNT          641 a         1347 c         1389 g          619 t
ORIGIN      
        1 atgttgtgct gatagccgac ctcgacgcga aaggtcgcca tgcccgggtc cggggcgcgc
       61 tcctggcgtt gcttcttcgg gcgctcgccg ccctcggggc gcggctcgaa gctgcgctcg
      121 ggccggcggt cgtcggcgcg ctcgggcggg cgctcgttgc tgcggtccgg cttgacgagc
      181 aacagcggct cgtcgccacg gaacaggcgg gcgagcgccg cggcgacctc gatcgcgggc
      241 acgttctcct cgcgctcgaa ctcctcgatg atgccgcggt acccgtcgag gtcgccgctg
      301 gcgagggtgt cgcggatctg ctgcttgaac ttgccgatcc ggacgtcgtt gacggccttg
      361 atcgtcggca gctgcatcag cgtgatcggc tgccgggtcg cgcgctcgat gatccgcagc
      421 aggttctgct cgcgcggcga gatgaacagg atcgcctcgc cgctgcggcc ggcgcggccg
      481 gtgcggccga tgcggtgcac gtagctctcc gggtcggtcg gcacgtcgaa gttgatcacg
      541 tggctgacgc ggtcgacgtc gaggccgcgc gcggcgacat cggtggcgac gaggatgtcg
      601 atcttgccgt cgcgcagcat cgcgatcgtg cgctcgcgct ggctctgctg gatgtcgccg
      661 ttgatcgcct ccgccgcgaa gccgcgggcc cgcaggcgct cggccagctc gtcggtcgac
      721 tgcttggtcc gcgcgaacac gatcatcgcg tcgaagggct cggcctcgag gatgcgggtc
      781 agcgcgtcga gcttgtgcat gccgctgacc tgccagaagc gctggcggat gttctccgcg
      841 gtccgcgtct tggccgcgat cgtgacctcg accggcttgc gcatgtaggt ctgcgcgatc
      901 cggcggatcg gcgccggcag ggtcgccgag aacaggcagg tctgccgctc cgccggggtc
      961 ttgcgcagga tcgtctcgac gtcgtcgatg aagcccatgc gcagcatctc gtccgcctcg
     1021 tcgagcacga ggtacttgag ctgcgagagg tcgagcgagc cgcgctcgag gtggtcgatg
     1081 atgcggcccg gcgtgccgac gacgacgtgc acgccgcggc tgagcgcctg gaactgcggg
     1141 ccgtagctct ggccgccgta gatcggcagg acgcgaaagc cgggcaggtg ggtcgcgtag
     1201 cgctggaacg cctcggcgac ctggatcccg agctcgcggg tcggcgtcag caccagcgcc
     1261 tgcggcggcc gcttgtcgag ctcgaggcgc gccaggatcg gcagcgcgaa ggcggcggtc
     1321 ttgccggtgc cggtctgcgc ctggcccagc aggtcgcgcc cggccatcag gtgcgggatc
     1381 gtctgggcct ggatcgggga gggcgtctcg tagccgacct cgcgtaacac ccgaagcagg
     1441 gcctccggca gctcgagccc gtcaaatccg gaggcgggcg tgacggtgtc ggtgtcttct
     1501 tgcatggccc gaggtctacc cgtttgcgcg cccgcgcgag aggcccctcg gtgtgcttcg
     1561 aggcacgtgg accgccgtcg cggggcgccg agacgtcaca aagctgacgg acgtggtcct
     1621 gtccgggtcg tccggacgcc tggcggcgct gcggcctccg gtcacagcga ggcccgcggc
     1681 ttgctccggt ccgggcccgg acttatgtac ctgcgggggc cgccatggaa cagaacatca
     1741 cgacgcacac cttcaagcgc gacgatacgt gggagacgat cgacacgctg tggtacagcc
     1801 cgttctttta ctggcaaaaa aacggcctgc aagtgacgcc gaaggtcccg ctgcggatca
     1861 tcacgttctt tggcacgttg atcgacgagt ccaacgaggg gtggatcaac accggcggac
     1921 cgagcgcgat cctcctgcag aagatccagg cccgtggcgt ccgcggccag acgatccgcg
     1981 tcgagaccgg cgaggagatc gtcgaggcac ccgagacctc ggcccgaagc tgacgcccgg
     2041 acgagtcgtg tgtcgcgcgg agggcgcgcg cgaccgtgca ccgatcgccg ggagccgcct
     2101 cctcgcgccg ctgggggcct ctgcgttgcg tcgcagacct ggccgcggtg gccgcccgcg
     2161 agcccccgtg acatagggca gcggtccatg ccactgcacc gacgtcgcgg caatgcatat
     2221 cccctcgggg ccacctggga cggtcacggg gtcaacttcg cgatctacag cgagaacgcg
     2281 accagcgtcg agctgtgcct catcgacgag gagggcatcg agacgcgggt gcgcctgcgc
     2341 gacccgaccg ccttcgtgtg gcacacctac ctcgagggca tcaagccggg gcagcgctac
     2401 ggctatcgcg tgtacgggcc gtgggagccg gagcgagggc tgcgcttcaa tccgcgcaac
     2461 ctgctgaccg acccctacgc gatggccttc agcggcgtga cggtatggga ggacggcgcc
     2521 ttctcgtacg acctcgagga cccctcggac gaccgcgacc tgacgatcaa tccccgcgag
     2581 agcctgggcg tgccgctgag cgtggtcgtc gaccgcagct tcaactgggg ggacgaccag
     2641 tcgcccaacg tgccgctgca cgggctgatc atctacgaga cgcacatcaa gggcctgacg
     2701 atgcgacacc cggacgtccc ggaggagctg cgtggcacct acgcggcggt cgccagcgac
     2761 ccgatcatca agcacctcaa ggagctcggc gtcaacgcga tcgagctgtt acccgtgcac
     2821 cagttcgtgg acgacaagca cctgctcgac cgcggcgtgc gtaactactg gggttacaac
     2881 tcgatcggct tcttcgcgcc ggacgtgcgc taccgctgcg gcgacgagat caccggcgag
     2941 gtgcgccagt tcaaggagat ggtgcggagg ctgcatcagg cgggcatcga ggtgatcctc
     3001 gacgtcgtct acaaccacac cgccgagggc aaccactacg gtccgacgtt tagcttcaag
     3061 ggcgtcgaca acccgaccta ctatcgcctg gtccccgaca acccgcggtt ttattacgac
     3121 tacacgggca ccggcaacag cctcaacgtg cggcacccgc agacgctgca gctgatcatg
     3181 gactcgctgc gctactgggt gaccgagatg cacgtcgacg ggtttcgctt cgacctcgcg
     3241 gccgcgctcg cgcgcgggct ctacgaggtc gagcagctgt cgagcttctt cacgatcatc
     3301 catcaggacc cgatcatcag ccaggtcaag ctgatcgccg agccgtggga cgtcgggccc
     3361 ggcgggtatc aggtcggcaa ctttccggtt cgctgggccg agtggaacgg caagtaccgc
     3421 gacacgatgc gcgccttctg gcgcggcgag ggcggcaagg ccggcgaggt cggctaccgc
     3481 ctcagcggca gctctgacct gtaccagaac gacggccggc gcccgtactc gagcatcaac
     3541 ttcatcaccg cgcacgacgg cttcacgctc gcggacctcg tctcctacaa cgacaagcac
     3601 aacgaggcca acggcgaggg cggcaacgac ggcgactcgc acaacctgtc gtggaactgc
     3661 ggcgtcgagg gtccgaccga cgacccggag gtgctcgcgc tgcgccgacg ccagcagcgc
     3721 aacctgatgg cgacgctgct gctctcgcaa ggcaccccga tgatcagcgg cggcgacgag
     3781 atcggtcgca cgcagcgcgg caacaacaac gcctactgcc aggacaacga gatcagctgg
     3841 tacgactggg acctcgacga cgaccgcaag gcgatgctgg ccttcacgca gcgcgtgatc
     3901 gggatctatc gcaagcaccc ggcgctgcaa cgggccaagt tcttcaaggg gcgaaagatc
     3961 cgcggcaccg acgtccgcga catcatgtgg ttcaac
//
LOCUS       DASWNJ010000936         3585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_300513, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000936 DASWNJ010000000
VERSION     DASWNJ010000936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300513"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VGB85_15850"
     CDS             <1..379
                     /locus_tag="VGB85_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional initiation protein Tat"
                     /protein_id="HEY0135559.1"
                     /translation="PIPDALGKHHRIDREHAKMFAHLLKRLSMYTTDAGTLLDDSVAV
                     WCNDLGTGVGHSYNNIPWVCAGGAGGFLKTGQYIDAGGVDHNKFHNTILSAVGLTNME
                     GGHVDDFGDPELEPGVIDAMIAV"
     gene            582..2045
                     /locus_tag="VGB85_15855"
     CDS             582..2045
                     /locus_tag="VGB85_15855"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEY0135560.1"
                     /translation="MTRLPRRVSPWLLALTLAGSLAAVGGCAGRQVTSGGIELRQGAA
                     SHELEALAVAQKGAAAVAVVSTDVGRGLAFVVDPRGYMLTNRHVVEDADYIDNVTFPA
                     LKPSRQFASARIIYIDPVRDLALIKVDAEDPLPYLTLGTARPRPIEDYLHFQDKVVLL
                     TRVAQNDSDGTGGGKTDKTQPGFIARTGKVQELEVYNKGVGTGAFFGLTHDVKKGMSG
                     GPVLDRYGRAVGVVSWTWKHRVGGYAIPISEAAQMLAERPVLDSAPQQASRAEARARG
                     FVTAVHGGQLEHARRMLSPTYARKIRSYTMRTIAEQVPGEGKVPVQQFVAALDDIVES
                     QEAGSDSLPFARVQDVVLRTGTPTFMAALGVDGLLSKEQVISFFFEFAQAYVSARHFG
                     DISSDAAMDIALNRLQTVDAARTFAFAEVAQRIGTGDSIEIEKVDLVPGAYAPQAVVR
                     LKVAHKADKASAQTFIMQLRLEWGDWYVAELQSVGLG"
     gene            2011..3396
                     /locus_tag="VGB85_15860"
     CDS             2011..3396
                     /locus_tag="VGB85_15860"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0135561.1"
                     /translation="MSRSCRASGSGDRAGTPGGLRSAATARQGFKASRPGDGASARWY
                     RRSVSHEPTAGSAELHDGSSSLHSEPELHDGRTSIQRLVVSNYRSLGRDVTITLGPLT
                     VLVGPNGSGKSNVVDALHFVADCMSMGLGGAINDRNGIESCRRWNRSHGGHPYNVSFR
                     LELMLPRGPATYEFELTGSNVAEYCIKSERAVVHDGQEHVLSVNAGKLITSPDGLVPA
                     VDDQNLVLPLVGGDLRFRPLFQALRSIAVYSIYPDTLRRPHHYSATKPMAKHGDNWVS
                     ILKDQPESTWKMDLVAALNKLTGDIEDIEIKRAGGLLVIRFRRTIEKKSSTAGKWFEA
                     YQESDGTLRVAGIITALLQQPPVPVIAIEEPELTVHPGAIRLVADFLKQASRTSQVLV
                     TTHSPELLDCVDASAIRVVTRSPERGTAVSHMGASQGEAVRRGLMSLGEVLRLEGFQT
                     ELPLPTDAGED"
     gene            3403..>3585
                     /locus_tag="VGB85_15865"
     CDS             3403..>3585
                     /locus_tag="VGB85_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135562.1"
                     /translation="MPSILVVEGQGELEAAHNLLVRLTREHGHDLQWARPRRFPNLHL
                     QRGILKAAEVIRIDPSA"
BASE COUNT          592 a         1309 c         1153 g          531 t
ORIGIN      
        1 cccgatcccc gacgcgctcg gcaagcacca ccgcatcgac cgcgagcacg ccaagatgtt
       61 cgcgcacctg ctcaagcgcc tgtcgatgta cacgaccgac gccggcacgc tgctcgacga
      121 cagcgtcgcg gtgtggtgca acgatctcgg gaccggcgtc ggccacagct acaacaacat
      181 cccgtgggtg tgcgcgggcg gggccggcgg cttcctcaag accggccagt acatcgacgc
      241 tggcggcgtc gaccacaaca agttccacaa cacgatcctc agcgcggtcg gcctgaccaa
      301 catggaaggc ggccacgtcg acgacttcgg cgaccccgag ctcgagcccg gcgtcatcga
      361 tgcgatgatc gcggtgtgaa ccccggccct cgcggggccc atgagcacgt gatcgcgcga
      421 tgggcgggca gatgtccgcc catcgtcgcg accgcaggtc cacgggcgag ggccacgcca
      481 gcccgacgca ggcgcgacgc cggccgcccg tgcgggcccc cggccacaga ttctcggccg
      541 ctccgctggc cgccccccgg gtccggcggt agcctgaaat ggtgacccgt ctgccccgcc
      601 gcgtctcgcc ctggttgctg gccctcaccc tcgccggcag cctcgccgcg gtcggcggct
      661 gcgccgggcg gcaggtgacg tccggaggca tcgagctccg gcaaggcgcc gcctcgcacg
      721 agctcgaggc cctcgcggtc gcccagaagg gcgccgccgc ggtcgcggtc gtctcgacgg
      781 atgtcggtcg cggcctcgcc ttcgtcgtcg accctcgcgg ctacatgctg accaaccgtc
      841 acgtcgtcga ggacgccgac tacatcgaca acgtcacctt cccggcgctc aagccgtcgc
      901 gccagttcgc cagcgcgcgc atcatctaca tcgacccggt ccgcgacctc gcgctgatca
      961 aggtcgacgc cgaggacccg ctgccctacc tgaccctcgg gacagctcgg ccgcggccga
     1021 tcgaagacta cctgcacttc caggacaagg tcgtgctgct gacgcgggtc gcccagaacg
     1081 acagcgacgg gaccggcggc ggcaagaccg acaagaccca gcccggcttc atcgcgcgca
     1141 ccggcaaggt ccaggagctc gaggtctaca acaagggcgt cggcaccggc gcgttcttcg
     1201 ggctgacgca cgacgtcaag aagggcatgt cgggcggccc ggtgctcgac cgctatggtc
     1261 gcgcggtcgg ggtggtctcg tggacctgga agcaccgcgt cggcggctac gcgatcccga
     1321 tctcggaggc cgcgcagatg ctcgccgagc ggccggtgct ggacagcgcc ccgcagcagg
     1381 ccagccgcgc cgaggcgcgc gcccgcggct tcgtcaccgc cgtgcacggc ggccagctcg
     1441 agcacgcccg gcgcatgctc tcgccgacct acgcgcggaa gatccgctcg tacaccatgc
     1501 gcacgatcgc cgagcaggtg cccggcgagg gcaaggtccc cgtgcagcag ttcgtggcgg
     1561 cgctcgacga catcgtcgag tcgcaggagg ccgggtccga cagcctgccg ttcgcccgcg
     1621 tccaggacgt cgtcctgcgc accggcacgc ccaccttcat ggccgcgctc ggcgtcgacg
     1681 gcctgctctc gaaggagcag gtgatcagct tcttcttcga gttcgcccag gcctacgtca
     1741 gcgcccgcca cttcggcgac atctcctccg acgccgcgat ggacatcgcc ctcaaccgcc
     1801 tccagacggt cgacgccgcc cgcaccttcg cgttcgccga ggtcgcccag cgcatcggca
     1861 ccggcgacag catcgagatc gaaaaggtcg acctcgtccc cggcgcctac gcgccccagg
     1921 ccgtcgtccg cctcaaggtc gcacacaagg ccgacaaggc gagcgcccag acgttcatca
     1981 tgcagttgcg gctcgagtgg ggcgactggt atgtcgcgga gctgcagagc gtcgggctcg
     2041 ggtgaccggg ccgggacgcc gggtgggctg aggtcggcgg ccacggctcg acagggcttc
     2101 aaagcgtcgc gcccgggtga cggggcgtca gcgcgctggt accgtcggtc cgtgtctcac
     2161 gagcccaccg ccggatccgc ggagttgcac gatggatcgt cttccctgca ctccgaaccc
     2221 gagcttcacg acggtcggac cagcatccag cgactcgtcg tatccaatta ccgcagcctg
     2281 ggacgtgacg taaccatcac gctcggaccc ctcaccgtgc tggtgggacc caacggctcg
     2341 ggcaagagca acgtcgtcga cgcgctccac ttcgtggccg actgcatgag catggggctc
     2401 ggcggcgcga tcaacgatcg taacggcatc gagtcctgcc ggcggtggaa ccgctcgcac
     2461 ggcggacatc cctacaacgt cagcttccgc ctcgagctga tgctaccgcg tggacctgcg
     2521 acctatgagt tcgagctcac gggctccaac gtcgccgagt actgcatcaa gtccgagaga
     2581 gcggtagtcc atgatggtca ggagcacgtc ctcagcgtca acgctgggaa attgatcacc
     2641 tcccctgacg gcctcgttcc cgcggtcgac gaccagaacc tggtgttgcc gctcgtcggc
     2701 ggtgacctgc gatttcggcc actttttcag gccctgcgca gcatcgcggt ctactcgatc
     2761 taccctgata cgctgcgccg tccgcaccac tacagcgcga ccaagccgat ggccaagcac
     2821 ggggacaact gggtctcgat cttgaaggat cagccagagt ccacgtggaa gatggacctg
     2881 gtcgcggcac tcaacaagtt gaccggcgac atcgaggaca tcgagatcaa acgcgctggt
     2941 ggcctcctcg tcattcgttt tcgccgcacg atcgagaaga agagcagcac cgccggaaaa
     3001 tggttcgagg cataccagga gtccgacggg accctgcgcg tcgccggcat catcacggcg
     3061 ttgcttcagc agccccccgt gcccgtgatc gccatcgagg agcccgagct gacggtccac
     3121 ccgggtgcga tccggctggt cgccgacttt ctcaaacagg ccagccgcac gtcacaggtg
     3181 ctcgtgacca cgcacagccc cgagctactc gactgcgtcg acgcctctgc gatccgcgtc
     3241 gtcacccgca gccccgaacg cggcaccgcc gtgagccaca tgggcgcctc ccagggggag
     3301 gccgtgcgcc gcgggctgat gtcgctcggc gaggtgctgc gcctggaggg ttttcagacc
     3361 gaactaccgc taccgacgga tgccggggag gactgatccg agatgccatc gatcctcgtc
     3421 gtcgagggcc agggcgagct cgaggcggcc cataacctgc ttgtccgact gacccgcgag
     3481 cacggtcacg acctgcagtg ggcccgacca cgccgctttc ccaatcttca ccttcaacgc
     3541 ggcatcctca aggctgccga ggtgatccgt atcgatccct cggcg
//
LOCUS       DASWNJ010000937         1942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_653038, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000937 DASWNJ010000000
VERSION     DASWNJ010000937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653038"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VGB85_15870"
     CDS             <1..320
                     /locus_tag="VGB85_15870"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0135563.1"
                     /translation="PQSTFRVGVATVDLARLTVTWPGGQVDLTAREGLILEYLRARPG
                     VAVTRDALLTEVWGYHDGSIRTRTVDVHILQLRTKLRAVPGGDAWIGTVRGRGYRFEA
                     EAA"
     gene            317..>1942
                     /locus_tag="VGB85_15875"
     CDS             317..>1942
                     /locus_tag="VGB85_15875"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0135564.1"
                     /translation="MSRPDARLRGAARRRVLALGLGVALPTVLLAVGWLLASLREQAG
                     LAREHQAERRQAAERIAAAVREGLEELRRREDARPFYLYNHFYSPPEVLAAGDAVAVS
                     PLAADPDDPRVLGHFQVDPGERVRTPYPVDDADPGPPRHRMLVATVGAPAFAPLRALS
                     FGTPAPAPDLRLADAGAPLTTNLGELSNQVYRELSEPQVSAPPKILRKQVPVTSRNQV
                     SWETIQSQQARNVPIAQANDAAPVFQQIAAQQAYGNADIARQVVPPEPVPVPEDMSDR
                     TAHRPTLAQGAEVDYTPMAYLGLGEHLVLQRVVSHAETGSVQGVLLDRAHLIDTWLPA
                     VAARHAPAGAAPQVVGDRSACAAPQPLADPLAGVWLCFAEPAPPPDHLAFQAGALLGL
                     IGVVGIGMVVVARAARRADELSAQKTAFVSAVSHELRTPLTTLRMHAELLQAGLVAPA
                     SLATIHDDLVRESVRLGRLVENVLAVSQLEEGRRVLDLRPGDLGEHLRDVIAGQARHV
                     ASKGFAPVDLVVPDDPVIVRFDAQAIEQIVVNLL"
BASE COUNT          238 a          812 c          644 g          248 t
ORIGIN      
        1 cgccgcagtc cacctttcgc gtcggcgtcg cgaccgtcga cctcgcgcgc ctcaccgtca
       61 cctggcccgg aggacaggtc gacctgaccg cccgcgaggg cctgatcctc gagtacctgc
      121 gcgcccgacc cggcgtcgcc gtcacgcgcg atgcgctgct gacggaggtc tggggctatc
      181 acgacggcag catccgcacg cgcaccgtcg acgtgcacat ccttcagctg cggacgaagc
      241 tgcgcgccgt gcccggcggt gacgcgtgga tcgggacggt gcgcggccgc ggctatcgct
      301 tcgaggcgga ggccgcgtga gccggcccga cgcgcggctg cgcggcgcgg cgcgccgacg
      361 agtgctcgcg ctcggcctcg gcgtcgccct gccgacggtg ctgctcgcgg tcggctggct
      421 gctcgcctcg ctgcgcgagc aggccggcct cgcccgcgag catcaggccg agcgccggca
      481 agccgcggag cggatcgcgg ccgccgtgcg cgagggcctc gaggagctgc gccgccgcga
      541 ggacgcccgg ccgttctacc tctacaacca tttctacagc ccgcccgagg tgctcgcggc
      601 cggcgacgcg gtggccgtct cgccgctcgc cgccgacccc gacgacccgc gcgtgctcgg
      661 gcacttccag gtcgaccccg gcgagcgcgt gcggaccccc taccccgtcg acgacgccga
      721 ccccggtccg ccgcgccacc gcatgctcgt cgcgaccgtc ggcgcgcccg ccttcgcccc
      781 gctgcgcgcg ctcagcttcg gcacccccgc gccggcgccc gacctgcgcc tcgccgacgc
      841 tggcgccccg ctgacgacca acctcggcga gctctcgaac caggtctacc gcgagctcag
      901 cgagccgcaa gtcagcgcac cgccgaagat cctccgcaag caggtcccgg tcaccagccg
      961 caaccaggtc agctgggaga cgatccagag ccagcaagcg cgcaacgtcc ccatcgccca
     1021 ggccaacgac gccgcgcccg tgttccagca gatcgccgcc cagcaggcgt acggcaacgc
     1081 cgacatcgcg cggcaggtcg tcccgcccga gcccgtaccg gtccccgagg acatgtcgga
     1141 tcggacagct catcggccga ccctggcgca gggcgccgag gtcgactaca cgccgatggc
     1201 ctacctcggc ctcggcgagc acctcgtgct ccagcgcgtc gtctcgcacg ccgagaccgg
     1261 ctccgtgcag ggcgtgctgc tcgaccgcgc gcacttgatc gacacctggc tgcccgccgt
     1321 cgccgcccgc catgcgcccg ccggcgccgc cccgcaggtc gtcggcgatc gctccgcctg
     1381 cgccgcgccg cagccgctgg ccgacccgct cgcgggcgtg tggctgtgct tcgccgagcc
     1441 cgccccgccg cccgaccacc tggcctttca ggcaggtgcc ctgctcggcc tgatcggcgt
     1501 cgtcgggatc ggcatggtcg tcgtcgcccg cgcggcccgt cgcgccgacg agctctcggc
     1561 ccagaagacc gcgttcgtgt cggccgtctc gcacgagctt cgcaccccgc tgacgaccct
     1621 gcgcatgcac gccgagctgt tacaagccgg gctggtcgcc cccgccagcc tcgccacgat
     1681 ccacgacgac ctcgtgcgcg agagcgtgcg cctcggccgg ctggtcgaga acgtgctcgc
     1741 cgtctcgcag ctcgaggagg gccgccgcgt gctcgacctc cgccccggcg acctcggcga
     1801 gcacctgcgc gacgtgatcg ccggccaggc ccgccacgtc gccagcaagg gcttcgcgcc
     1861 ggtcgacctc gtcgtccccg acgatcccgt gatcgtccgc ttcgacgccc aggcgatcga
     1921 gcagatcgtc gtcaacctcc tc
//
LOCUS       DASWNJ010000938         6791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_399624, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000938 DASWNJ010000000
VERSION     DASWNJ010000938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399624"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1397
                     /locus_tag="VGB85_15880"
     CDS             <1..1397
                     /locus_tag="VGB85_15880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135565.1"
                     /translation="RQLASELLREQASVIVIDDQHFQLYDMAMNGVSFLAPPEAPPWQ
                     VDDVLEVDIRVHETSAYKGRARVAREERVYGQRRVGLQLLTGFLDLHEMQRVDEEQAL
                     LHDLIDGPERIIAKIPIAYREALLAAVHFAAFYQRILDYHEARLQDAHAIAEEREALA
                     RRAIVAIRPRWNTLRLAAAEAALSVRGDRQALIAAKTATETLLTPLVTAAPLLHRSYH
                     KPLGYAGDFSVMLHIYNDRFEGSTAFGQVFHKLACEEPLSAGVRTRKDFVKRITAEEH
                     TRRRAEGHVLRALSLGCGPAREVVEYLAEGEAPPHGVHWTLIDQEEKALSVAYHDVLR
                     GINQRQAGCTAQCLYLSFEQLIRDPLAVRGEPQDLVYCVGMFDYLNRRRAQALLVALY
                     ERVSSGGLLVIGNALGPNTHFWMSEFAVDWTLIFRDREELRQLAEPVVKAGAVLDIQR
                     EPSDAYDFILLRKP"
     gene            1446..2624
                     /locus_tag="VGB85_15885"
     CDS             1446..2624
                     /locus_tag="VGB85_15885"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY0135566.1"
                     /translation="MPTPTAVDFEASLLPRNASALRIEVRLGVMLVPAFWALDWVVLP
                     ERIWLLLWIRAVPTTFGLVILLLSRWAPGWFERHANALAFTFSLMVACSIILMCFLHQ
                     GYESAYYAGVNLLILGVGLLFSWRAVTAILFNAAVYACYMAPLALGLVTISDFPIALT
                     NQFFLVATAIITVASMSHRRGLERREFDAQIAQQNLLAEVQTMATIDWLTNLYNRRHF
                     FRLGEEEIARARRYRHPISVIMIDIDHFKSVNDTHGHSVGDEVLCTIAKRILAGLRQS
                     DIAGRYGGEEFAIVLPETDLPSAANIVAERLRDTIAAHAVHTAEGPLHITISVGVAGV
                     DLERENLLDALSRADAGLYAAKHAGRNRVIVWTPELARPASESLSLIVAEPAPQVLVS
                     "
     gene            2743..4776
                     /locus_tag="VGB85_15890"
     CDS             2743..4776
                     /locus_tag="VGB85_15890"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0135567.1"
                     /translation="MDSTLAAAPGEAGEHFSDLAVGATIGRYVIRERLGQGAMGVVYL
                     ANDPDLGREVALKMLKSSGLRDERVARVRLLREAQSLARISQHNVVGVHDVGISDGTV
                     FIAMELVHGVTLTQWLKHQPRRWSEIIDIFVSIGTGLAAAHQAGIVHRDFKPENVLVG
                     HDDLPKVTDFGLAHAAEAATEAPPEAPPATAPLDALEVSLTATGMLVGTPAYMSPEQF
                     LRHPTDARSDQFSFCVALWEALYGRHPFQNDTLAALAVSVTTGDIATPTDTRGVPQWV
                     QDCVLRGLSVAPGDRWPTMLALLAALERRSSFDASRAPRDVRALRLEAILGITFVPGF
                     WILDWFVIPHAVWISLTLRMLCLAYGVAILVLLAQRPRWVELHVHSLAVSFSLLVAWS
                     IALMCFLHVGYESSYYAGINLLVICVGQLYLWPLPRALLFTLGVYCFYMLPLALGYIN
                     VTDPIAALSNQFFLVSTMTVTVIAQVHRRHVESSDFDAHIERGRLEQQVLTDALTGLY
                     NRAQFLRASAEEMERSRRYGQPLCLLVVDVDRLQVINETLGSQAGDEVLISIAARIVA
                     AVRRQDIVSRHGGTFAVLLVNSQAGAVTVIADRIRSSICAPPVPTSVGTCNVTAKIGF
                     TEITVETDNILEAFAQAGAMLDAAKHGGAPIQSWSARTKSSAHSKLRDTDSEG"
     gene            complement(4806..5177)
                     /locus_tag="VGB85_15895"
     CDS             complement(4806..5177)
                     /locus_tag="VGB85_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135568.1"
                     /translation="MNTSRWMMVAALAGGLLAGACSEDVSVHVACVSTAAPAVECQVE
                     ERVGKLEVEVCWDFGITCANGGVVKAERTCQKVKDGATAKVVIPLEKLTGFDTCGGEG
                     TPKAQVTNITLNGRPQEPLVK"
     gene            5284..>6791
                     /locus_tag="VGB85_15900"
     CDS             5284..>6791
                     /locus_tag="VGB85_15900"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0135569.1"
                     /translation="MADPETEPGDPGSAPTEMASPGAPPLAHRLARAELKERLFGAPL
                     ESAHIGRYSILRRLGAGGMGVVYAAYDEQLDRKIAIKLVHPDRHDPDSRARTRREAQA
                     LARLSHPNVVQVYEVGEHEGQVYLALEFVQGRTLRTWQTEAPRRWQDTLAMYLQAGKG
                     LAAAHARGLVHRDFKPDNVLVDDEEGRPRVLDFGLARVPGTSELSPGTGGSPVTGDES
                     ARLTTPGTVLGTPAYMPPEQLAGGEADARSDVFSFCAALHEALHGVRPFTGEHRESLH
                     AAILRGEVQRPARAAEVPAWLRDMVESGLAADPAARWQAMDPLLTALARGPARRRRRA
                     LLLAGCLTAAIVAVVIVLVARRDPQAAAVLDDRSAQVRDELAQTRVARADEQRHGEAR
                     RLATLAGVRGQQDPVTGLLLALEAVATHTRVDAPPTVEAEQALLDALDSVRSRPFLRP
                     GATMVDAVAESPDGRWLVTGERDGSVTLWPTDSPRQANVLLAGDGHPVRALA"
BASE COUNT         1045 a         2497 c         2160 g         1089 t
ORIGIN      
        1 gacggcagct agcgagcgag ctgctgcgcg agcaagcatc ggtcatcgtc atcgacgacc
       61 aacactttca actctacgac atggccatga acggcgtgtc gttcctcgcc ccgccggaag
      121 ctccgccgtg gcaagtcgac gacgtgctcg aggtcgacat ccgcgtccac gaaaccagcg
      181 cgtacaaggg tcgcgcccgc gtcgcccgcg aggagcgggt ctacggccag cgccgcgtcg
      241 gcctccagct gctgaccggc ttcctcgatc tccacgagat gcagcgcgtc gacgaggagc
      301 aggcgctcct gcacgacctg atcgacgggc ccgagcgcat catcgccaag atcccgatcg
      361 cctatcgcga ggccttgctc gccgccgtcc acttcgccgc gttctaccag cgcattctcg
      421 actaccacga ggctcgtctg caagacgcgc acgcgatcgc cgaggagcgc gaggcgctgg
      481 ctcgccgagc gatcgtggcg atccgcccgc gctggaacac actgcgcctc gccgcggccg
      541 aagccgccct ctcggtccgt ggcgatcgtc aggcgctgat cgccgcaaag accgccaccg
      601 agacgctgct gacgccgctt gtcactgcgg cgccgctgct ccaccgctcg taccacaagc
      661 cgctcggcta cgccggcgac ttctcggtca tgctgcacat ctacaacgac cgcttcgagg
      721 gctccaccgc gttcgggcag gtgttccaca agctcgcctg cgaggagccc ttgagcgccg
      781 gtgttcgcac ccgcaaggac ttcgtcaaga ggatcaccgc cgaggagcac acccggcgcc
      841 gcgctgaagg gcatgtgcta cgcgcgctga gcctcggctg cggcccggcg cgagaggtgg
      901 tcgagtacct cgccgagggc gaggctccgc cccatggcgt gcactggacc ttgatcgacc
      961 aggaggagaa agcgctgagc gtggcgtacc acgacgtcct gcgcggcatc aaccagcgtc
     1021 aagccggctg caccgcacag tgcctgtacc tgtcgttcga gcagctcatc cgcgatcccc
     1081 tcgcggtgcg cggcgagccc caggacctgg tgtactgcgt agggatgttc gactacctca
     1141 accggcggcg cgcccaggcg ctgttggtgg cgctatacga gcgcgtcagc tccggaggcc
     1201 tgctggtgat cggcaacgct ctcgggccta acacgcactt ctggatgtcg gagttcgcgg
     1261 tggattggac gctgatcttc cgcgaccgcg aggagctgcg tcagctcgcc gagccggtcg
     1321 tcaaggccgg ggccgtgctc gacatccagc gcgaaccctc ggacgcctat gacttcatct
     1381 tgctgcgcaa gccgtgagtc gacgataccg cgagtcatgc gccgaggccg tgagacgagg
     1441 agatgatgcc aacgccgacc gcagtcgact ttgaggcgag cctgctgccg cgcaacgcgt
     1501 cggcgctgcg catcgaagtg cggctcggcg tgatgctcgt gccggcgttc tgggccctcg
     1561 actgggtcgt gctgccagag cggatctggc tgcttctgtg gatccgcgcg gtcccgacga
     1621 ccttcggcct ggtgatcctg ctgctgagcc gatgggcacc cggatggttc gagcgccacg
     1681 caaatgccct cgccttcacc ttctccctga tggtggcctg ctcgatcatc ttgatgtgct
     1741 tccttcatca gggctacgaa tcagcctact atgcgggggt aaacctgctg atcctcggtg
     1801 tcggcctcct gttctcgtgg cgcgccgtga cggcgatcct gttcaacgcg gcggtctacg
     1861 cctgctacat ggccccgcta gcgctcggcc tcgtgacgat atccgacttc ccgatcgcgc
     1921 tgacgaacca gttcttcctc gtcgccaccg cgatcatcac cgtcgcctcg atgagccacc
     1981 gacgcggcct cgagcgtcgc gagttcgacg cccagatcgc ccagcagaac ctgctcgcgg
     2041 aggtccagac gatggcgaca atcgactggc tgacgaacct ctacaaccgc cgccacttct
     2101 ttcgcctcgg cgaggaggag atcgcccgcg ctcgccgcta ccgccacccg atcagcgtga
     2161 tcatgatcga tatcgaccat tttaagtcgg tcaatgacac tcacggacac tccgtcggcg
     2221 acgaggtcct gtgcacgatc gccaagcgga tcctcgcggg gctccgccag agcgacatag
     2281 cgggccgcta cggtggcgag gagttcgcca tcgtgctgcc cgagaccgac ctgccgagcg
     2341 ccgcgaacat cgtcgccgaa cgcctgcgcg acacgatagc cgcgcacgca gtccatacgg
     2401 ccgaggggcc cctccatatc accatcagcg tcggtgtcgc tggcgtcgac ctcgagcgtg
     2461 agaacctcct cgacgccctg agccgtgcgg acgccggcct gtacgccgcc aaacacgccg
     2521 gccgcaatcg tgtgatcgtc tggacccccg agctcgcccg gcccgcctcc gaatcgttat
     2581 cgttgatcgt cgccgagccc gccccacagg tcctggtaag ttagcggcgc gtggcgtacg
     2641 ctcgctggag ggcagtcgac tgcgctgctc tgcgacgcag ggcgaaccca gaggccctct
     2701 atgagcgaag cgccactcag tgcgaccctc ccggcggagg ccatggactc caccttggcc
     2761 gccgctccgg gcgaagctgg cgagcacttt tcagacctcg cggtcggcgc gacgatcgga
     2821 cgctacgtga tccgcgagcg gctcggtcag ggggccatgg gcgtcgtcta cctcgccaac
     2881 gaccccgatc tcggacgcga agtggctctg aagatgctca agagctccgg cctacgcgac
     2941 gaacgggtgg cgcgtgtccg cctgctgcgc gaggcccagt ccctggcgag gatctcccag
     3001 cataacgtgg tcggcgtcca tgacgtcggg atcagcgacg gcacggtgtt catcgccatg
     3061 gagctggtcc acggcgtcac cctcacccaa tggctcaagc accagccccg gcgatggagc
     3121 gagatcatcg acatcttcgt ctccatcggt actggcctcg ccgccgctca ccaggccggc
     3181 atcgtgcacc gcgacttcaa gcccgagaac gtcctggtcg gccacgacga cctcccgaaa
     3241 gtcaccgact tcggcctcgc ccatgcggcc gaggccgcca ccgaggcgcc acccgaggca
     3301 ccacccgcca ccgcccccct cgatgcgctg gaggtctcgc tcactgcgac aggcatgttg
     3361 gtcggcacgc ccgcgtacat gtcccccgaa caatttctgc gccatccgac cgacgcacgc
     3421 tcggatcagt tctcgttctg tgtcgccctc tgggaggcac tgtacggccg gcatccgttc
     3481 cagaacgaca ccctggcagc gctggccgtc tcggtgacca ccggcgacat agcgacgccg
     3541 accgataccc gcggggtacc gcagtgggtg caggactgcg tcctgcgcgg gctctccgtc
     3601 gctcctggag accgctggcc gacgatgttg gctctcctgg ccgcgctcga gcgcaggtcc
     3661 agcttcgacg ccagccgcgc gccgcgggac gtgcgagcgt tgcggctcga ggccatcctc
     3721 gggatcacct tcgttccagg gttctggatc ctcgactggt tcgtgatccc gcacgcggta
     3781 tggatctccc tcacgctgag gatgctctgc ctcgcctatg gcgtcgccat cctcgttctg
     3841 ctggcacagc gcccgcgatg ggtggagttg cacgtgcact ccctcgcggt atctttctcg
     3901 ctcctggtgg catggtcgat cgcgttgatg tgttttctac acgtcggcta cgaatcttcc
     3961 tactacgcgg gcatcaatct cctggtgatc tgcgtcggcc agctatacct atggcccttg
     4021 cccagggcgc tcctgttcac gctcggcgta tattgttttt acatgctgcc gctggcgctc
     4081 ggctatatca acgttacgga cccgatcgcg gccctgtcca atcaattttt cctcgtcagc
     4141 acgatgaccg tgactgtgat cgctcaagtc catcgccgac acgtcgagag cagcgatttc
     4201 gacgcgcaca tcgagcgcgg gaggctcgaa cagcaggtcc tcaccgacgc tctcacgggg
     4261 ctgtacaacc gggcgcagtt cctgcgtgcc agcgccgagg agatggagcg ctcacgccgg
     4321 tacgggcaac cgctgtgcct cctggtggtc gatgtcgatc gcctgcaggt catcaacgag
     4381 acgctcggca gccaggcagg agacgaggtg ctgatcagca tcgcagcgcg gatcgtcgcc
     4441 gcagttcgcc gccaagacat cgtgagtcga catggcggca cctttgccgt gctactcgtc
     4501 aacagtcagg ccggggcggt gaccgtgatc gccgatcgga tccggagctc catttgcgct
     4561 cctccggtcc ccacgtccgt ggggacgtgc aacgtgaccg ccaagatcgg tttcacggag
     4621 atcacggtgg agaccgataa tatcctcgag gccttcgcac aggcgggtgc catgctcgat
     4681 gccgcgaagc acgggggcgc tccgatccag agctggtccg cgcggacgaa gtcgtcagcc
     4741 cactcgaagc tccgagacac cgattccgaa ggttgagcgg gcccgcgccc gcctgaccca
     4801 ctacctcact tgaccagcgg ctcctgcggc ctgccgttga gcgtgatgtt cgtgacctgc
     4861 gccttcggcg tcccctcgcc gccgcacgtg tcgaagcccg tcaacttctc cagcgggatc
     4921 acgaccttgg ccgtggcgcc gtccttgacc ttctgacagg tgcgctccgc cttgaccacg
     4981 ccaccgttcg cgcaggtgat cccgaagtcc cagcagacct cgacctcgag cttgccgacg
     5041 cgctcctcga cctgacactc gacggcgggc gcggccgtcg acacgcacgc cacgtgcacc
     5101 gagacatcct cggagcacgc cccggcgagc agacctccgg cgagcgcagc gaccatcatc
     5161 cagcgggagg tgttcatcgg ttcacgatag aggatctcgc ccgcgggctc aatgcggccg
     5221 cccctcgcgc gttcacgacc gccatccgcg cggacacggg ttttcgctac agtcgtcgcc
     5281 gcgatggcag acccggagac cgagcccggc gaccccggca gcgcgccgac cgagatggcg
     5341 tcccccggcg ccccgccgct ggcccatcgg ctcgcgcgcg ccgagctcaa ggagcggctg
     5401 ttcggcgcgc cactggagtc ggcccacatc ggtcgctatt caatcctgcg caggctcggc
     5461 gccggtggca tgggggtcgt ctacgcggcc tacgacgagc agctcgaccg caagatcgcc
     5521 atcaagctgg tccaccccga tcgccacgac cccgactcgc gggcccgcac ccgccgcgag
     5581 gcccaggccc tcgcacgttt gtcgcacccc aacgtcgtcc aggtctacga ggtcggcgaa
     5641 cacgagggcc aggtctacct ggcgttggaa ttcgtgcagg gccggaccct gcgcacctgg
     5701 cagaccgagg ccccgcgccg ctggcaagac accctcgcca tgtacctgca agccggcaag
     5761 ggcctcgccg ccgcgcacgc ccgcgggctc gtacaccgcg acttcaagcc cgacaacgtg
     5821 ctcgtcgacg acgaggaggg ccgcccccgc gtcctcgact tcggcctcgc gcgcgtgccc
     5881 ggcaccagcg agctgtcccc ggggacaggt ggctcgccgg tgacgggcga cgagtcggcg
     5941 cgcctgacca cgccgggcac ggtgctcggc acgcccgcgt acatgccccc ggagcagctc
     6001 gccggcggcg aggccgacgc gcgcagcgac gtgttcagct tctgtgcggc gctccacgag
     6061 gcgctccacg gcgtgcgccc gttcaccggc gaacaccgcg agagcctgca cgctgcgatc
     6121 ttgcgcggcg aggtgcaacg gcccgcgcgc gcggccgagg tgccggcgtg gctacgcgac
     6181 atggtcgagt cgggcctcgc cgccgacccg gccgcgcgct ggcaggcgat ggacccgctg
     6241 ctgaccgcgc tggcccgcgg cccggcccgc cggcgccggc gcgcgctgtt gctcgccggg
     6301 tgcctgacgg ccgcgatcgt cgcggtcgtc atcgtgctcg tcgcacgcag ggacccgcag
     6361 gccgcggccg tgctcgacga ccgcagcgcg caggtgcgtg acgagctcgc gcagacccgg
     6421 gtcgcccgcg ccgacgagca gcgccacggc gaggcccgcc gcctcgccac gctggcaggt
     6481 gtccgcggcc agcaggaccc cgtcaccggc ctgctgctcg cgctcgaggc cgtcgccacg
     6541 cacacccgcg tcgacgcccc accgaccgtc gaggccgaac aggcgctgct cgacgcgctc
     6601 gacagcgtgc gcagccgccc gttcctgcgg cccggcgcca ccatggtcga cgccgtcgcc
     6661 gagagccccg acggtcgctg gctggtcacc ggcgagcgcg acggcagcgt cacgctgtgg
     6721 cccaccgaca gtccgcgcca agccaacgtc ctgctcgcgg gcgacggcca ccccgtgcgc
     6781 gcgctggcct t
//
LOCUS       DASWNJ010000939         2088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_613656, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000939 DASWNJ010000000
VERSION     DASWNJ010000939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613656"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VGB85_15905"
     CDS             <1..658
                     /locus_tag="VGB85_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HEY0135570.1"
                     /translation="ILKDEALRAEYDLWFELGIFGVPYAANDEVPGCAGRNDTQLGTL
                     LLYTTSTAEPQQFTTLLPLAISQPFNNYAEGASDMVGLTRKTISFDLPADTAETQLVL
                     ITSNHGANQGGEEYIRRQHYVYVDQALVLQYKPGRTSCEPFRVYNTQGNGIYGASPRS
                     DEEWQSFSNWCPGDIIDTRIVPLGPVGAGKHELVIDVPDATFVGAQGDFPFSLYIQAR
                     "
     gene            748..1536
                     /locus_tag="VGB85_15910"
     CDS             748..1536
                     /locus_tag="VGB85_15910"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HEY0135571.1"
                     /translation="MSAGQRQHEQTARGERPRGPWGATWSAHIDRLGDFAGRRERGRE
                     YLIKGAVTELAVEPGRISARVQGSRSYHPRIVVQPLSEAMRAGFLELTCAGGCSLRDL
                     LQAARVTPQCGLLPSLRELSAVCTCPDRVRPCKHIFAALYAVGRRLDAEPELLLALRG
                     VALDDLRGEPPRRPRRSAAAKQRPVAAPRAPRITRASAQQQAAKTVRVGSRRVRREEL
                     RALGVPAPTIDGWLRQGTLLRTAERGVYRRTAALERRLAARGVG"
     gene            complement(1533..>2088)
                     /locus_tag="VGB85_15915"
     CDS             complement(1533..>2088)
                     /locus_tag="VGB85_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135572.1"
                     /translation="LTAFSWVARFTSLGLTAGTGDCTGSDCDGKYTAAIALLYMAPIS
                     QFVATGLVIPGGVIKGRHDGWRRITTGNPERNGKAYVVAGGVMFGVFTALSVALRPVY
                     VAALVNCVGESIEGSNSDCGGVGGYVGYNIGVAVSDAGSTAGAGLMSYGLGYRGYMRR
                     HGAQLSVAPFGSRGAYGLSLTARF"
BASE COUNT          310 a          804 c          688 g          286 t
ORIGIN      
        1 gatcctcaag gacgaggcgc tgcgcgccga gtacgacctg tggttcgagc tcggcatctt
       61 cggcgtgcct tacgccgcga acgacgaggt ccccggctgc gccggccgca acgacacgca
      121 gctcggcacc ctgctgctgt acaccaccag caccgccgag ccgcagcagt tcaccacgct
      181 gctgccgctg gcgatcagcc agcccttcaa caactacgcc gagggcgcca gcgacatggt
      241 cgggctcacc cgcaagacga tcagcttcga cctacccgcg gacaccgccg aaacccagct
      301 cgtgctgatc acctccaacc atggcgcgaa ccagggcggt gaggagtaca ttcgccgcca
      361 gcactacgta tatgtcgacc aggccctcgt gctgcaatac aagccgggcc gcaccagctg
      421 cgagccgttt cgcgtctaca acacccaggg caacggcatc tacggcgcct cgccgcgcag
      481 cgacgaggag tggcagtcgt ttagcaactg gtgcccgggc gacatcatcg acacgcgcat
      541 cgtgccgctc ggcccggtcg gcgccggcaa gcacgagctc gtgatcgacg tgcccgacgc
      601 gaccttcgtc ggcgcccagg gagatttccc gttctctctg tacattcagg cccggtagcc
      661 cggcgcggcc ctcgccgcgg ggcccctgcg cgattttgat cgatcaccac tggcaggcgg
      721 gcgaaatcgc gctacggtct gcggatcatg tccgcgggcc agcgacagca cgagcagacg
      781 gcacggggcg agcgcccccg cggcccgtgg ggcgcgacct ggtccgcaca tatcgaccgc
      841 ctcggcgact tcgcgggccg ccgcgagcgc ggccgcgagt atttaattaa aggcgcggtg
      901 accgagcttg cggtcgagcc cggccggatc tcggcgcgcg tgcagggcag ccgctcctat
      961 cacccgagga tcgtcgtgca gccgctctcg gaggcgatgc gcgccggctt tctcgagctc
     1021 acctgcgcgg gcggctgctc gctgcgcgac ctgctgcagg ccgcccgcgt gaccccgcag
     1081 tgcggcctgc tcccgtccct gcgcgagctg tcggccgtgt gcacctgccc cgaccgggtc
     1141 cgtccgtgta aacacatctt cgcggccctc tacgcggtcg gacgccgcct cgacgccgag
     1201 ccggagcttc tgctcgccct ccgcggcgtc gccctcgacg acctgcgcgg cgagccaccg
     1261 cgccggccgc ggcgcagcgc ggccgcgaag cagcgccctg tcgctgcccc gcgggcccct
     1321 cgcatcaccc gcgcgtccgc ccaacagcaa gcggcgaaga ccgtgcgggt cgggtcgcgc
     1381 cgggtgcggc gcgaggagct gcgtgcgctc ggcgtcccgg ccccgacgat cgacggctgg
     1441 ctgcgccagg gcaccttgct gcgcaccgcg gagcgcggcg tgtatcgccg caccgccgcg
     1501 ctcgagcgcc ggctcgccgc gcgcggcgtc ggctaaaagc gtgcggtcag gctcagcccg
     1561 tacgcgcccc ggctgccgaa cggcgcgacg ctgagctgcg ccccgtgccg ccgcatatat
     1621 ccgcggtatc cgagcccgta ggacatcagg ccggcaccgg ccgtgctgcc ggcgtcgctg
     1681 accgccacgc cgatgttgta accgacgtag cctccgaccc cgccgcaatc gctgttggac
     1741 ccctcgatgg actccccgac gcagttgacc agcgccgcga cgtacaccgg acgcagcgcg
     1801 accgacagcg cggtgaacac accgaacatc acgccgccgg cgacgacgta ggccttgccg
     1861 ttgcgctcgg gattgcccgt ggtgatccgc cgccacccgt cgtgcctgcc cttgatcacc
     1921 ccgcccggga tcaccaggcc ggtggcgacg aactgcgaga tcggggccat gtacagcagc
     1981 gcgatcgcgg cggtgtactt gccgtcgcag tccgaccccg tgcagtcacc ggtcccggcc
     2041 gtgaggccca gcgaggtgaa gcgcgccacc cagctaaacg ccgtcagg
//
LOCUS       DASWNJ010000940         2412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_158114, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000940 DASWNJ010000000
VERSION     DASWNJ010000940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158114"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..349)
                     /locus_tag="VGB85_15920"
     CDS             complement(212..349)
                     /locus_tag="VGB85_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135573.1"
                     /translation="MGSTVIVVVGADVPVGSAPVLVLLLVLLLVLLVLLVPPVDPSLP
                     S"
     gene            339..1340
                     /locus_tag="VGB85_15925"
     CDS             339..1340
                     /locus_tag="VGB85_15925"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY0135574.1"
                     /translation="MEPTSGESSTTGTTFDDSTSSSGSETGETGFDPGIEGDGDYVIG
                     PDYMDAPEIDPGPDVPRGDIFHFTMKSVDSAIFPGVMGEYTRDVWIYVPKQYVDGTAA
                     PFIVVQDGGGYLDLTSATLDTLIDAKQVPAMIGVFINPGPGDGMGSERGLEYDRVSED
                     YTNFIETEVLPIIPAVPEIAAAYPNLAFTDDPWGRASMGGSSGGACAFTMGWLRPDLY
                     RRILTYSGTFVAQHSTPEYPHGAWEYHEHLVAGADEKPLRVFLEVGEYDLDLNANFGD
                     GMHAWVEANNAMAAALAAKTYHYRYLFAKNAGHVDGKVLRQTLPDTLRWLWVGYPVE"
     gene            complement(1378..2196)
                     /locus_tag="VGB85_15930"
     CDS             complement(1378..2196)
                     /locus_tag="VGB85_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135575.1"
                     /translation="MLRISSLLPAFVLSFLAACGDDGDGGKYKSCDDVVCGDHASCGG
                     YLAECSCNVGYAGDPYDRCFAEQPKSCDELVCGDNATCDGDPGECSCDAGYAGDPYKK
                     CIAEQPPADETCALECGQNAYCSEGECFCELDHVAVCGANAGCLPEARLCDHQQDCPN
                     AADEAVVVCSTPLFQDWLLTDDCDDGLPIEWRLFAQDREWSWPDGASSYKTTGLGAED
                     HQTVQCFEGEYICFGGRSGAVTWGFNLDGSGSCDACCAACGSQSVLDIGFLTCD"
BASE COUNT          413 a          839 c          812 g          348 t
ORIGIN      
        1 accacagctg ggccgtcctg accacaaccg ggcagaccgc agccgcgcga tcacgcatcg
       61 cgtgacatct gcgagtgggc gatcgcagtc atcgaccgct ccgcggtcga tcgacccggg
      121 atcgccggac cgggcgcgca ctctcacgta tcatcgcggc gatgttccga ccgatgactt
      181 tcgccgcgtg tgcactgctg atgttcgcct gttacgacgg gagtgagggg tcgaccggcg
      241 gcacgagcag cacgagcagc acgagcagca gcacgagcag cagcacgagc accggcgccg
      301 agccgactgg cacatcggcg ccgacgacca cgatgacggt ggagcccacc tcgggcgagt
      361 cgagcacgac cggcacgacg ttcgacgaca gcaccagcag cagcgggagc gagaccggcg
      421 agaccggctt cgatccgggg atcgagggcg acggcgacta cgtgatcggg ccggactaca
      481 tggatgcgcc cgagatcgac cccggacccg acgtgccgcg cggcgacatc tttcacttca
      541 cgatgaagtc ggtcgacagc gcgatcttcc cgggcgtgat gggcgagtac acgcgcgacg
      601 tgtggatcta cgtgcccaag cagtacgtcg acgggaccgc ggcgcccttc atcgtcgtgc
      661 aggacggcgg gggatacctc gacctcacct cggcgaccct cgacacgctg atcgacgcca
      721 agcaggtccc ggcgatgatc ggcgtgttca tcaaccccgg ccctggcgac ggcatgggca
      781 gcgagcgcgg cctcgagtac gaccgcgtgt cggaggacta caccaacttc atcgagaccg
      841 aggtgctgcc gatcattccc gcggtccccg agatcgcggc cgcgtatccg aacctcgcgt
      901 tcaccgacga tccatggggt cgcgcgtcga tgggcggcag ctcgggcggc gcgtgtgctt
      961 tcacgatggg ctggctgcgc ccggacctgt atcgcaggat cctgacctac tcgggcacct
     1021 tcgtcgccca gcacagcacg cccgagtacc cgcatggcgc ctgggagtac cacgagcacc
     1081 tcgtcgccgg ggccgacgag aagccgctgc gcgtgttcct cgaggtcggc gaatacgacc
     1141 tcgacctcaa cgccaacttc ggcgacggca tgcacgcctg ggtcgaggcc aacaacgcga
     1201 tggccgccgc gctcgcggcg aagacctatc attaccgcta cctcttcgcc aagaacgccg
     1261 gccacgtcga cggcaaggtg ctgcgccaga cgctgcccga caccctgcgg tggctgtggg
     1321 tcggctaccc ggtcgagtga tcgggacgcg ccggcggcgg cgtccgcttc tcctggatca
     1381 gtcgcaggtc aggaagccga tgtccagcac gctctggctg ccacacgccg cgcagcaggc
     1441 gtcgcagctg ccgctgccgt cgaggttgaa gccccatgta acggccccgg atcggccccc
     1501 gaagcagata tactcgccct cgaagcactg cacggtctga tggtcctcgg cgcccaggcc
     1561 ggtggtcttg tagctgctgg cgccgtccgg ccacgaccac tcgcggtcct gcgcgaacag
     1621 ccgccactcg atcggcaggc cgtcgtcgca gtcgtcggtc agcagccagt cctggaacag
     1681 cggcgtcgag cagaccacga cggcctcgtc ggcggcgttc gggcagtcct gttgatgatc
     1741 acagagccgg gcctcgggca gacagccagc gttcgcgcca cagacggcga cgtggtcgag
     1801 ctcgcagaag cactcgccct ccgagcaata cgcgttctgg ccgcactcga gcgcgcaggt
     1861 ctcgtcggcg ggcggctgtt cggcgataca tttcttgtaa gggtcccccg cataaccggc
     1921 gtcgcaggag cactcgcccg ggtcgccgtc gcaggtggcg ttgtcgccac agacgagctc
     1981 gtcgcagctc ttgggctgct cggcgaaaca gcgatcatag gggtcccccg cgtaaccgac
     2041 gttgcaggag cactccgcca ggtagccgcc gcagctggcg tggtcgccac agacgacgtc
     2101 gtcgcagctc ttgtacttcc cgccatcacc gtcgtcccca caagcagcga ggaacgagag
     2161 gacgaaggcc gggagcaggg aggagatgcg gagcatgtcg agccgtccgt aacgctgcgc
     2221 acggccgcga gcttcacgac cgtggggtgt cgaggtatgg ccaaaagggt gcgcgacccg
     2281 ggcccgcctg gcaacgggga tcgcggggtc gacggcgagg gcacgactcg ccgtgatacg
     2341 gtgcgctcgt gcctccgcgc cctgagctcg ccgtcacccg acacctcgtc ggccgcgaag
     2401 atcgcgcggc ac
//
LOCUS       DASWNJ010000941        10092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_653261, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000941 DASWNJ010000000
VERSION     DASWNJ010000941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 10092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10092
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653261"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..571)
                     /gene="bfr"
                     /locus_tag="VGB85_15935"
     CDS             complement(107..571)
                     /gene="bfr"
                     /locus_tag="VGB85_15935"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEY0135576.1"
                     /translation="MKGNDKVIQTLNDVLTAELTSINQYFIHAKMCQNWGFTKLAKKI
                     RDESIDEMKHADKLIERILFLEGVPNVQRLNKVNVGETVREQFELDLKLELDAISRFN
                     AGISLARDVGDNASRELLERMLISEEEHTDWLETQLSLITQLGEPMYCAQQL"
     gene            complement(744..953)
                     /locus_tag="VGB85_15940"
     CDS             complement(744..953)
                     /locus_tag="VGB85_15940"
                     /inference="COORDINATES: protein motif:HMM:NF016230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY0135577.1"
                     /translation="MIVCLCKGVSSRTILDEARRGACTLKQIRQSCQAGTDCGACVKQ
                     IRQLLDTVSARHEHHDAAVGVSEDR"
     gene            complement(1476..3311)
                     /locus_tag="VGB85_15945"
     CDS             complement(1476..3311)
                     /locus_tag="VGB85_15945"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY0135578.1"
                     /translation="MRLVSVLLRHRPILASTGLLALAGLLAVGHAPDHNGVAIADLPA
                     LDMTAVGDAGTGALLLDLVEPEDGEGGSEAELAALLADLGLAYEPAGFYSEGEHLYRV
                     FGDEAALTALQARLAGNPLVEVVEPELMFSLLDESDPEANKRQRERFVPDDPLFSRQW
                     HMEMIHTPEAWKITAGKGAVVAVIDTGVAWKDLPGTAKQVPDLAGTAFVHGESFVPGG
                     LPDGLDDHLHGTHVAGTIAQTTNNAIGVAGVAFDSTIMPLKVLSGDGRGSVPGIANAI
                     RFAADHGAHVINMSLGGPLPSAVLGKAVIYAHEKGVTVVCAAGNEKRSRVGYPAGHDG
                     AVAVAAVDAAGERTFYSNWGNALDISAPGGDTRADKNGDGFPDGILQNTIMLQNPMQN
                     DYMWLQGTSMASPHAAGVAALIVSRGITNPAEVERIMKRTAKHPNNVEWDKDYGAGVI
                     DALAAVDASTESYAPERAGLAGLLGLAGLASLGVAGAVTRRGRVVAAASMVAGAGLAA
                     GAFGCAPLAYQLAAASGSVAAFGSPLVLSALLPLLFTLLLLGVRPLRSLLVGINLGYA
                     ALLLHGAIVLPTLVAGLPGGAGIDRLWLAVNALLALALARRVSRV"
     gene            3503..3579
                     /locus_tag="VGB85_15950"
     tRNA            3503..3579
                     /locus_tag="VGB85_15950"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3537..3539,aa:Arg,seq:tcg)
     gene            complement(3659..6070)
                     /locus_tag="VGB85_15955"
     CDS             complement(3659..6070)
                     /locus_tag="VGB85_15955"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0135579.1"
                     /translation="MRILHLSDLHRAGSAETLKAIWNGPQGALRKLPEAEQRFDLVVV
                     SGDLAGSATPAEYAELHEFAVTSLCPLLREPECHARIVFVPGNHDVDWGAELGQPLRI
                     ADMLQKPGGSEQLARLLKRYREDPARSGVRQVISKYGHIEWIALDESRQSARFANLQR
                     FFTDFYGDSLREPGRCFDLSAAQEGLDWSAHIFPEDRVAVYGFNSCFLNDRHWTGAAI
                     SRESIANAAVHASEHAEGFLRIAVWHHGIHTDSYRPDYLNQADLGALIVAGFQVGFHG
                     HLHKAGAQQLGWLTDRFVLVGTGSLGANQNQRPDAVGKQFSVVQIHPHQVHVQVFERT
                     GDMAVYTRRPARTLPLGSSTEREQHEISAGEHQRIAHVDRHGIITVGVRIIGLSTAHP
                     IPVAELNPPVCEARQESPQGSPGFYIRQRTAQDGTIRFTLYPPDDRRTDLVWRYQASN
                     AIPLTQAEVPYYNLDGTRNLDGTRNSMVTRGHTVRFPCRHLTLTFTYDDPVVAMDTVE
                     KWALRAVTHNGEQHWERVDSEERRCELIKHSDTKISLAIEAPIVGHRYAIAYRPSLVG
                     HALDFTGGRIAAKLLDRCIADRELGSILSYQLAETIAAALAAVFGASPAGVSWMGLLW
                     DEAQGKLLPAFGHFASREWVCSYPCGTGVAGHAFRFNRPAAWSRGTAHSKDALVYQRS
                     PTYQHWKPEHDWIVCVPLVGDTHKNPLGVVQFEGGGKQQGLGDRLREFANAALAQAVT
                     KGSPWEVFQQTLSSTVNTGFWQACALADCLVDYRDHTRKLIGELGLGGQPDDASLTDG
                     SMIRL"
     gene            complement(6156..6407)
                     /locus_tag="VGB85_15960"
     CDS             complement(6156..6407)
                     /locus_tag="VGB85_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135580.1"
                     /translation="MADEAAHARQTDISTQNDLGAVALLPEPFKVNAEAVDLAAAAAR
                     AALRRTSAAIGRHARSTLPSQASPHVDPFLSAVEPAKLP"
     gene            complement(6516..>10092)
                     /locus_tag="VGB85_15965"
     CDS             complement(6516..>10092)
                     /locus_tag="VGB85_15965"
                     /inference="COORDINATES: protein motif:HMM:NF024904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0135581.1"
                     /translation="RSPYGFRYPYQAMAIKATLLDSAGQRIELVRRKKRKDALLGNLR
                     TDAGEVTLDEPRFRRYFGALTEELYKAVFGFTHTDLQQGADVLKVAGLGELLGGGALG
                     GGAEVVRRVLGELRDEGEALFKSRGKNPPINRNLAQLRDARNSLREATYQQPQYLALT
                     QALREAREVLATADTELSRLRLRGARVQTLLNTFEDFHEHQRLRARLADPGLATPLQP
                     ADAERVRPLLTDLRAREQRHQALTDELERLERRAAQHPVDHALLVEAAAVDRLLRRVE
                     PIAAQRRELPRERDRLEREREALREQFDRLSHGTAALSPGTAPAAISAAELEQVRVAL
                     GRWRRARQELQVLARGEADDEAELTALRTEAALLADQAPDERGAALLAELERAQADHA
                     ELERGERELARLDAEIDLLLARLHPRPTGPLDRLPLPGFATVQDMQSRHLDRERERRD
                     AHEHFSQQTSELAQIETEIAAVTGEDLPDPAQLAEARRRRDETWATVRQQLLARGTTP
                     QRDLFSPTEPGLPEPAPRVGDRRTLARGFERALADADQLADRLRASADRLAQRTALTH
                     TAERARVALTRARERLASLDAAERAWTAEWAELWRPTGVPPESPRSMLAWLGDATTLR
                     DLAAQRARREHGLAPLRPQVRYFEARLRDHLGPSAPHTATTAANTSPATTPPSHDAGS
                     PAPRGVAAYLSPSTSPTSHAAHFAAAASAYTTSSPALTWPALVAQLRERERVAQTRRG
                     RLESTQARATALAAALARTTTARSFLERDLATAADDLRRRTAALGLAADLDPETAVER
                     LEALADLSERLSSNELRRTACEQALAALTTFDAEVAALHARLARAPDPGDPPEQQVER
                     LSRALASAREAATTHALARDEAADKRTEQQRLAAALADARGEYEALLARAGVHDEAAL
                     SAMAERTLLRDQLTRSCADLDLRLARALGEGDAREAYEAELRASRREALVSEQQTLTR
                     EISELDQRRISAALAKGRHEHEAQQLGGDRAARVGAEIEVIKSELEEQIDRYLLVHLA
                     ERVLDRVTDRYARENQPAILQYTSELLARITEGRHLRVVVQPETRSLATVDRQGQLRQ
                     PGDLSTGTREQMFLAQRLAYILDHCDRSEPLPVIMDDVLVNFDDARAAASLATLRDAA
                     RATQVILLTCHRRWLEIARTVAPDARILELPAESPPAGVLRTAEA"
BASE COUNT         1356 a         3124 c         4098 g         1514 t
ORIGIN      
        1 aagcccgccg cgggttagcg cgacggaccc ggcgcaagca aaagcccgcc gcgggtcagc
       61 gcggcgggcg gcggacgact cggtgatccg gctccccggg ctcccttcag agctgctggg
      121 cgcagtacat cggctcgccg agctgcgtga tcaggctgag ctgggtctcg agccagtcgg
      181 tgtgctcttc ctcggagatc agcatgcgct cgagcagctc gcgggaggcg ttgtcgccga
      241 cgtcgcgggc cagcgagatg ccggcgttga agcggctgat cgcgtcgagc tcgagcttga
      301 ggtcgagctc gaactgctcg cggacggtct cgccgacgtt gaccttgttg aggcgctgca
      361 cgttcgggac accctcgagg aacaggatgc gctcgatcag cttgtccgcg tgcttcatct
      421 cgtcgatcga ctcgtcgcgg atcttcttcg ccagcttggt gaagccccag ttctggcaca
      481 tcttcgcgtg gatgaagtac tggttgatgc tcgtgagctc ggccgtcagc acgtcgttga
      541 gggtttgaat gaccttgtcg ttgcccttca tcgtgacggc aagctaacac ggtcgcggcg
      601 gtcacgggca tggggacatg cacggggatc gcggcctgca ccgacgctga gatcgacttt
      661 caggaccggc ctgcccggat gccccgcagg cgctgcgaat gagagccgct ttcagcggcg
      721 caccaccggc tcaacgagct gcctcaacgg tcttcgctga cgccgaccgc ggcgtcgtga
      781 tgctcgtggc gggccgagac ggtgtcgagg agctgccgga tctgcttcac gcaggcgccg
      841 cagtcggtgc cggcctggca ggactgccgg atctgcttca gcgtgcaggc cccacggcgc
      901 gcctcgtcca gaatggtccg gctcgaaact cctttgcaca ggcagacgat catggcggcg
      961 gttccctcga cttccggtcc cggtgagcgg cactgaaagt cattctcaga atggtcaacc
     1021 agatctaccc gagcggccag gcccggacaa gggcctgaaa acgattttca ttcgcgcgtg
     1081 ctgccgcgca acccgaccag gacgcgggtt ttcggcggga cttgagggtt tgcggggtgt
     1141 gcgcgcgcag gcgtggtcgt ccgcacggcg cgaagccgcg cgttttgcgt gggcgggaga
     1201 ggtcccgccc cccggtcagt gtgggccgga tgcgagaccg gccaggccga tgccgacggt
     1261 cggcatgcgc gctcgagcgg ccgatgtcga gggtcggcac gtggctcgag cggaacggtg
     1321 tcgagggtcg gcacggggca cgaggttggt atgcgagcgg gacgcccggc ctcgcttaag
     1381 cagtcaggcc gggctcgacg agccgagggc tgcccctttt taaaaggaac aaggcccgca
     1441 gcgagggctg cgggcctttc gaggtcagcc agcggtcaca cgcgagagac gcggcgggcg
     1501 agcgcgaggg ccaggagggc gttcaccgcg agccacaggc ggtcgatgcc ggcgccgccc
     1561 gggaggccgg cgacgagcgt cggcaggacg atcgcgccgt gcagcagcag ggcggcgtag
     1621 ccgaggttga tgccgaccag caggctgcga agcgggcgga ccccgagcag cagcagcgtg
     1681 aacagcagcg gcagcagggc cgagagcacc agcggcgagc cgaacgcggc gacgctgccg
     1741 gaggcggcgg cgagctggta ggccagcggc gcgcagccga aggcacccgc ggcgaggccg
     1801 gcaccggcga ccatgctggc ggccgcgacg acgcgaccac ggcgggtcac ggcaccggcg
     1861 acgccgaggc tggccaagcc ggcgaggccg agcaggcccg cgaggccggc gcgctcgggc
     1921 gcgtagctct cggtggaggc gtcgacggcg gcgagggcat cgatgacgcc ggcgccgtag
     1981 tccttgtccc actcgacgtt gttggggtgc ttggccgtgc gcttcatgat gcgctcgacc
     2041 tcggcggggt tggtgatgcc ccgcgagacg atcagcgcgg cgacgcccgc ggcgtgcggc
     2101 gaggccatcg aggtcccctg cagccacatg taatcgttct gcatcgggtt ctgcagcatg
     2161 atcgtgttct gcaggatgcc gtcggggaag ccgtcgccgt tcttgtcggc gcgggtgtca
     2221 ccgccggggg ccgagatgtc gagggcgttg ccccaattcg agtagaaggt gcgctcaccg
     2281 gcggcgtcga ccgccgcgac cgcgaccgcg ccgtcgtggc cggcggggta gccgacgcgc
     2341 gagcgcttct cgttgccggc ggcgcacacg acggtgacgc ccttctcgtg ggcgtaaatc
     2401 acggccttgc cgagcacggc cgacggcagc gggccgccga ggctcatgtt gatcacgtgc
     2461 gcgccgtggt cggccgcgaa gcggatcgcg ttggcgatgc cgggcaccga gccacggccg
     2521 tcacccgaga gcaccttgag cggcatgatc gtcgagtcga aggcgacacc ggcgacaccg
     2581 atcgcgttgt tggtggtctg cgcgatcgtg ccggcgacgt gggtgccgtg caggtgatcg
     2641 tcgaggccgt ccggcagccc gccggggaca aaggactcgc cgtgtacgaa ggcggtgccg
     2701 gcgaggtcgg gcacctgctt ggcggtgccg ggcaggtcct tccaggcgac gccggtgtcg
     2761 atgacggcga cgacggcacc cttgccggcg gtgatcttcc acgcctcggg ggtgtggatc
     2821 atctccatgt gccactggcg cgagaacagc gggtcgtccg ggacgaagcg ctcgcgctgg
     2881 cgcttgttgg cctccgggtc ggactcgtcg agcagcgaga acatcagctc gggctcgacg
     2941 acctcgacca gcgggttgcc cgcgaggcgg gcctgcagcg cggtcagggc ggcctcgtcg
     3001 ccgaacacgc ggtacaggtg ctcgccctcg ctgtagaagc cggccggctc ataggccagg
     3061 ccgaggtcgg cgagcagcgc ggcgagctcg gcctcgctgc caccctcacc gtcctcgggc
     3121 tcgacgaggt cgagcagcag ggcgccggtg ccggcgtcgc cgacggcggt catgtcgagg
     3181 gccgggaggt cggcgatggc gaccccgtta tggtcgggcg cgtggcccac ggcgagcagg
     3241 ccggcgaggg cgaggaggcc agtgctggcg aggatcgggc ggtgacgcaa gaggaccgag
     3301 acgaggcgca tggacaactc ctgaagcgac gaagtgacga accggagcag tataggagcg
     3361 cgggctgccc ggtgcacggc ggcaatgcga cggcgaggag accgcctgcg accgccctcg
     3421 cgcgagatcg ggtttcacgg tgcggtcccg gatcgtggcg gccttcacgc ccggccgagt
     3481 ttgggttagg ctccgggccc ctgcgcttgt agctcagctg gatagagcat cgggcttcga
     3541 acccgagggt cgcaggttcg aatcctgccg agcgcaccaa ggccccacgc acccgcgtgg
     3601 ggccttcttc tttgtcgacg tgatttcgcc cgaacggggc tgtccccggg gacatgtcct
     3661 aaaggcggat catgctgccg tcggtcagcg aggcgtcgtc gggctggccg ccgaggccga
     3721 gttcgccgat cagcttgcgg gtgtggtcgc ggtagtcgac caggcagtcc gcgagcgcgc
     3781 aggcctgcca gaagccggtg ttgacggtcg agctcagcgt ctgctgaaac acctcccacg
     3841 gcgagccctt ggtgacggcc tgagcgagcg cggcgttggc gaactcgcgc aggcgatcgc
     3901 cgaggccctg ttgtttgccg ccgccctcga actggacgac gccgagcggg ttcttgtggg
     3961 tgtcaccgac gagcggcacg cagacgatcc agtcgtgctc gggcttccag tgctggtagg
     4021 tcgggctgcg ctgatacacg agcgcgtcct tgctgtgggc ggtgccgcgg gaccaggcgg
     4081 ccgggcggtt gaagcgaaac gcgtggccag cgacgccggt gccgcagggg taggagcaga
     4141 cccactcgcg gctggcgaag tggccgaagg cgggcagcag cttgccctgg gcctcgtccc
     4201 acagcaggcc catccacgac acgcccgccg gcgaggcgcc gaacacggcc gcgagcgcgg
     4261 cggcgatcgt ctccgcgagt tgataggaga ggatcgaccc gagctcgcgg tcggcgatgc
     4321 agcggtcgag cagcttggcg gcgatccgtc cgccggtgaa gtcgagcgcg tgaccgacga
     4381 ggctcgggcg gtaggcgatc gcgtaacgat gcccgacgat cggggcctcg atcgccagcg
     4441 agatcttcgt gtccgaatgt ttaatgagct cgcagcgacg ctcctcgctg tcgacgcgct
     4501 cccagtgctg ctcgccgttg tgggtgacgg cgcgcagggc ccacttctcg acggtgtcca
     4561 tcgcgacgac ggggtcgtcg taggtgaagg tcagcgtcag gtggcggcac gggaagcgca
     4621 cggtgtgacc gcgtgtcacc atgctgttgc gggtgccgtc gaggttgcgg gtgccgtcga
     4681 ggttgtaata ggggacctcg gcctgggtca gcgggatcgc gttgctggcc tggtagcgcc
     4741 atacgaggtc ggtccggcgg tcgtcgggcg ggtagagcgt gaagcggatc gtgccgtctt
     4801 gcgcggtgcg ctggcggatg taaaagccgg gcgagccctg cgggctctcc tgccgcgcct
     4861 cacacacggg cgggttgagc tcggcgaccg ggatcgggtg cgcggtgctc aggccgatga
     4921 tgcgcacgcc gaccgtgatg atgccgtggc gatcgacgtg ggcgatccgc tggtgctcgc
     4981 cggcggagat ctcgtgctgc tcgcgctcag tactgctgcc gagcggcagc gtgcgcgcgg
     5041 gccggcgagt gtagacggcc atgtcgccgg tgcgctcgaa cacctgcacg tggacctggt
     5101 gcggatggat ctggaccacc gagaactgtt tgccgaccgc gtcggggcgc tggttctggt
     5161 tggcgccgag cgagccggtg ccgacgagca cgaagcggtc ggtgagccag ccgagctgct
     5221 gcgcgccggc tttatgtaag tggccgtgaa agccgacctg gaagccggcg acgatcagcg
     5281 cgccgaggtc ggcctggttg aggtagtcgg ggcggtaact gtcggtgtgg atgccgtggt
     5341 gccacacggc aattcttaaa aagccctcgg cgtgctcgga ggcgtgcacg gccgcgttgg
     5401 cgatcgactc gcgcgaaatg gccgcgccgg tccagtggcg gtcgttgagg aagcaggagt
     5461 tgaagccgta gacggcgacg cggtcctcgg gaaaaatgtg ggccgaccag tcgaggccct
     5521 cctgcgcggc ggacaggtcg aagcagcgac cgggctcgcg caggctgtcg ccgtagaagt
     5581 cggtgaaaaa gcgttgcagg ttggcgaagc gggccgactg gcgtgactcg tcgagcgcga
     5641 tccactcgat gtggccgtac ttcgagatta cctgacgcac gccggagcgg gccgggtcct
     5701 cgcggtagcg ttttaataag cgggcgagct gctcgctgcc gccgggcttt tgcagcatgt
     5761 cggcgatgcg caggggctgg ccgagctcgg cgccccagtc gacgtcgtgg ttgccgggca
     5821 cgaagacgat gcgggcgtgg cactcgggtt cacgcagcag cgggcacagg ctggtgacgg
     5881 cgaactcgtg cagctcggcg tactcggccg gcgtcgcgga gccggcgagg tcaccgctga
     5941 cgacgacgag gtcgaagcgc tgttcggcct ccggcagctt gcgcagggcg ccttgcgggc
     6001 cgttccagat cgccttgagg gtctcggccg agccggcgcg gtggaggtcg gagagatgaa
     6061 ggatgcgcac tggatctgcg gggcggacga ccctcggcgg atcttagcgg atctgcgcgg
     6121 agactggagg ggtcgcgcgg agcctcgcgg ggctctcagg ggagcttggc cggctcgacg
     6181 gcggagagga aggggtcgac gtgggggctg gcctggctcg gtagggtact gcgcgcgtga
     6241 cgaccgatgg cagcggaggt gcgacgcagg gcggcccggg cggccgcggc ggcgaggtcg
     6301 acggcctcgg cgttgacctt gaagggctcg ggtagcaagg ccaccgcgcc gaggtcgttt
     6361 tgggtcgaaa tgtcggtctg cctggcgtgc gctgcttcgt cggccatcgc ggggcctccc
     6421 gtatccagag taaggcgcca ggaaccccgc gtgtcaagcc tggggcgttt cgcgggcggg
     6481 ggaggtcggg gtttgcaagg tcgatgccgg gcggctcagg cctcggcggt tcgcagtaca
     6541 ccggcgggtg gcgactcggc gggcagctcg aggatgcggg cgtcgggggc gaccgtgcgt
     6601 gcgatctcca gccagcggcg gtggcaggtc aggaggatga cctgggtggc gcgggcggcg
     6661 tcccggagcg tggcgaggct ggcggccgcg cgggcgtcgt cgaagttgac gagcacgtcg
     6721 tccatgatga ccggcagcgg ctcgctgcga tcgcagtggt cgaggatgta ggcgaggcgc
     6781 tgggcgagga acatttgctc gcgtgtcccc gtggacaggt cgccgggctg gcgcagctgg
     6841 ccctggcgat cgacggtggc gagcgagcgg gtctccggct ggacgacgac ccgcaaatgt
     6901 cggccctccg tgatgcgggc cagcagctcg gaggtgtatt gcaagatcgc gggctggttc
     6961 tcgcgggcgt agcggtcggt gacgcggtcg agcacgcgct cggcgaggtg cacgagcagg
     7021 tagcgatcga tctgctcctc gagctcgctc ttgatgacct cgatctcggc gccgacgcgg
     7081 gcggcccgat ctccgccgag ctgctgggcc tcgtgctcgt ggcggccctt ggccagcgcg
     7141 gcggagatcc ggcgctggtc gagctcggag atctcgcggg tcagggtctg ttgctcgctg
     7201 acgagggcct cgcgccggct cgcgcgcagc tcggcctcgt aggcctcgcg cgcgtcgcct
     7261 tcacccaatg cgcgggcgag gcgaagatcg aggtcggcgc acgagcgcgt gagctggtcg
     7321 cgcagcaggg tccgttcagc catcgccgag agggcggctt cgtcgtggac gccggcccga
     7381 gccaacaacg cctcgtactc gccgcgcgcg tcggcgaggg cggcggcgag gcgctgttgc
     7441 tccgtgcgct tgtcggcggc ctcgtcgcgg gcgagcgcgt gggtggtggc ggcctcgcgg
     7501 gcgctggcga gggcgcggct caggcgttcg acctgctgct cgggtgggtc gcccgggtcg
     7561 ggggcgcggg cgaggcgggc gtgcagggcg gcgacctcgg cgtcgaaggt ggtcagcgcg
     7621 gcgagggcct gctcgcaggc ggtgcggcgc agttcgttgc tggagagacg ttcggagagg
     7681 tcggcgaggg cctcgaggcg ctcgaccgcg gtctcggggt cgaggtcggc ggcgaggccg
     7741 agcgcggcgg tgcggcggcg caggtcgtcg gcggcggtcg cgaggtcgcg ctcgaggaag
     7801 ctgcgcgcgg tggtggtgcg agcgagcgcg gcggcgaggg cggtcgcgcg ggcctgcgtc
     7861 gactcgaggc ggccgcggcg ggtctgggcg acgcgctcgc gctcgcggag ctgggcgacg
     7921 agcgcgggcc aggtgagcgc gggggatgag gtggtgtacg cggacgcggc ggctgcgaag
     7981 tgggcggcgt gcgaggtggg tgaggtcgag ggggagaggt atgcggcgac gcctcgagga
     8041 gcgggcgatc cggcgtcgtg cgacggtggt gtggtcgcag gcgaagtgtt cgcggccgtt
     8101 gtggcggtgt gaggcgcaga tgggccgagg tggtcgcgga ggcgagcctc aaagtagcga
     8161 acctgcgggc gcagcggggc gaggccgtgc tcgcgacggg cgcgctgggc cgcgaggtcg
     8221 cgcagggtgg tcgcgtcgcc gagccaggcg agcatcgagc gcggggactc gggtggcacg
     8281 ccggtcggcc gccacagctc ggcccactcg gccgtccagg cgcgctcggc cgcgtcgagg
     8341 ctggcgaggc gctcgcgggc gcgggtgagg gcgacgcgcg cgcgctcggc ggtgtgggtg
     8401 agggctgtcc tttgggccag gcggtcggcg ctggcgcgga ggcggtcggc gagctggtcg
     8461 gcgtcggcga gggcgcgctc gaagccgcgg gcgagggtgc ggcggtcgcc gacgcggggg
     8521 gctggctcgg gcaggccggg ctcggtcggg ctgaacaggt cgcgctgggg cgtggtgccg
     8581 cgggcgagca attgctggcg cacggtggcc caggtctcgt cgcggcgccg acgggcctcg
     8641 gccaattgcg cggggtcggg cagatcttcg ccggtgaccg cggcgatctc ggtctcgatc
     8701 tgcgcgagct cggaggtctg ttgcgagaag tgctcgtgcg cgtcccggcg ctcgcgctcg
     8761 cggtcgaggt gccgcgactg catgtcctgc acggtggcga agcccggcag cggcaggcgg
     8821 tcgagcggcc cggtcggccg cgggtgcagg cgcgcgagca gcagatcgat ctcggcgtcg
     8881 aggcgggcca gctcgcgctc gccacgttcg agctcggcgt ggtcggcctg ggcgcgctcg
     8941 agctcggcca acaacgcggc cccgcgctcg tcgggggcct gatcggcgag cagggcggcc
     9001 tcggtgcgca gggcggtcag ctcggcctcg tcgtcggcct cgccgcgggc gagcacctgc
     9061 agctcctgtc gggcgcggcg ccagcggccg agcgcgacgc gcacctgctc gagctcggcg
     9121 gccgagatcg cggcgggagc tgtcccgggg gacagggcgg ctgtcccgtg ggacaggcgg
     9181 tcgaattgct cgcgcaaggc ctcgcgctcg cgctcgaggc ggtcgcgctc gcgggggagc
     9241 tcgcggcgct gggcggcgat cggctcgacg cgacgcagca ggcgatcgac ggcggcggcc
     9301 tcgaccaaca acgcgtggtc gacggggtgc tgggcggcgc gacgctcgag gcgctcgagc
     9361 tcgtcggtga gggcttgatg gcgctgctcg cgggcgcgca ggtcggtcaa cagcggacgc
     9421 acgcgctcgg cgtcggcggg ctgcagcggc gtggcgagcc cgggatccgc gaggcgggcg
     9481 cgcaggcgct gatgctcgtg aaagtcctcg aaggtattta atagggtctg cacgcgggcg
     9541 ccgcggaggc gcaggcggga cagctcggtg tcggcggtgg cgaggacctc gcgggcctcg
     9601 cggagcgcct gggtcagcgc gaggtactgc ggctgctggt aggtcgcctc gcgcagggag
     9661 ttgcgggcgt cacggagctg cgcgaggttg cggttgatcg gtgggttttt gccgcggctc
     9721 ttgaacaggg cctcgccctc gtcgcgcagc tcgccgagca cgcggcgtac gacctcggcg
     9781 ccgccgccga gggcaccacc accgagcagc tcgccgaggc cggcgacttt caaaacgtcg
     9841 gcgccttgct ggaggtcggt gtgggtgaag ccgaagacgg ctttataaag ctcctcggtg
     9901 agggcgccga agtagcggcg aaagcggggc tcgtcgaggg tgacctcgcc ggcgtcggtg
     9961 cggaggttgc cgagcagggc gtccttgcgt tttttgcggc gcacgagctc gatgcgctgg
    10021 ccggcgctgt cgagcagcgt cgctttgatc gccatcgcct ggtacgggta gcgaaagccg
    10081 taggggctgc gc
//
LOCUS       DASWNJ010000942         2629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_229016, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000942 DASWNJ010000000
VERSION     DASWNJ010000942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229016"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VGB85_15970"
     CDS             complement(<1..666)
                     /locus_tag="VGB85_15970"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0135582.1"
                     /translation="MQPRKIALITGTSSGIGLHAAVALARAGYTTVATMRDLGKAGPL
                     RAAAAAAGVELELAPLDVTDLASITAAVAATLARHGRIDLLVNNAGAGFLGSLEHTSA
                     DDLQRVFAVNFFGTWEMTRAVLPAMRAAGAGRIIQVTSVGGVVGQPFNDAYCAAKFAV
                     EGMVEALAPVVHGFGVDVTLFEPGAVASEFVANLGASLAAREAAVDDPYAPMIAAYLD
                     RAGG"
     gene            795..1805
                     /locus_tag="VGB85_15975"
     CDS             795..1805
                     /locus_tag="VGB85_15975"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY0135583.1"
                     /translation="MISGLAEHGVDVVAIAGQRGVTAQQLMELLPLALVDEILACCFA
                     SCADPGLGLKLGAQIRPELFGVVGFAAMSCARLGEALERICRYKRALAGDRLTLRRSG
                     EAAVLRVELTTSAGVYARSKLDGEFAFLVGFARRMTGAPVLPLRVTYATPAPADMREH
                     AALFGCAPEFMADHNELWLPATELLRPLVGADPTLAALFVIQAEQLLRLAQGDSLTDR
                     VRGALAELLRGEAPGVAAVARRLGLSERSLQRRLREEGTSFAALVDATRQELATHYLC
                     DADLPAAEVAFLLGFAGAPSFYRAFRRWTQTTPEAYRAGRRGEAQVAVPRGRSVTVTL
                     GA"
     gene            1838..2089
                     /locus_tag="VGB85_15980"
     CDS             1838..2089
                     /locus_tag="VGB85_15980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135584.1"
                     /translation="MSAKKTEKEMWTPWASRHPEAQNVVITELTTRGFGSELRARKVF
                     KDCGYSKVRADFYQDLDSDKTREIDIVARLPVEELHDAE"
     gene            2086..2484
                     /locus_tag="VGB85_15985"
     CDS             2086..2484
                     /locus_tag="VGB85_15985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135585.1"
                     /translation="MSEFNSICSRSDAKAPVLTSSLTRHEKHPEDAWYSAAVAVFKAV
                     CDAGVGEAEAARKRRGKHEERPDVKVVTPLIILDGNLLCADLGEQGELELTPCDHIQL
                     SFTFSSKEYGRSRLHSHIVTMAAGRHGGER"
BASE COUNT          407 a          840 c          979 g          403 t
ORIGIN      
        1 gccgcccgcg cggtcgaggt aggcggcgat catcggcgcg taggggtcgt cgacggcggc
       61 ctcgcgggcg gccagcgacg cgccgaggtt ggcgacgaac tcgctggcga cggcgccggg
      121 ctcgaacagg gtcacgtcga cgccgaagcc gtggaccacc ggcgcgaggg cctcgaccat
      181 gccctcgacg gcgaatttgg cggcgcagta ggcgtcgttg aagggctggc cgacgacgcc
      241 gccgacgctg gtgacctgga tgatgcggcc ggccccggcg gcgcgcatcg ccgggagcac
      301 ggcgcgggtc atctcccagg tgccgaagaa gttgacggcg aagacgcgct ggaggtcgtc
      361 ggcgctggtg tgctcgagcg agccgaggaa gccggcgccg gcgttgttga ccagcaggtc
      421 gatgcggccg tgccgggcga gggtcgcggc gacggcggcg gtgatcgagg cgaggtcggt
      481 gacgtcgaga ggtgcgagct cgagctcgac gccggctgcg gccgcggctg cgcgcagggg
      541 cccggctttg ccgaggtcgc gcatggtggc gacggtggtg tagccggcgc gggcgagcgc
      601 gacggcggcg tggaggccga tgccggacga ggtgccggtg atgagagcga tcttcctggg
      661 ttgcatggat ctcccggtcg ccttgccggc gacgcggaag cagggatacg ctgcgcgtgc
      721 tcgccttgca ttcgccggac ccgccaaagc gttcgctccc gccgccaggc gcccacgcga
      781 ggatcccccg cgccttgatc tcggggctgg ccgagcacgg cgtcgacgtg gtcgcgatcg
      841 cggggcagcg cggggtgacg gcgcagcaat tgatggagct gctgccgctg gcgctggtcg
      901 acgagatcct ggcgtgctgc ttcgccagct gcgcggaccc ggggctcggg ctcaagctag
      961 gcgcgcagat ccgtcccgag ctgttcggcg tggtcggctt cgcggcgatg agctgcgcgc
     1021 ggctcggcga ggcgctcgag cggatctgcc gctacaagcg ggcgctggcc ggcgatcgcc
     1081 tgacgctgcg ccgcagcggc gaggcggcgg tgctgagagt cgagctgacg acgtcggcgg
     1141 gggtgtacgc gcgcagcaag ctcgacggcg agttcgcgtt cctggtcggc ttcgcccgcc
     1201 gcatgaccgg ggcgccggtg ctgccgctgc gcgtcacgta cgcgacgccg gccccggccg
     1261 atatgcgcga gcacgcggcg ctgttcgggt gtgcgcccga attcatggcc gaccacaacg
     1321 agctgtggct gccggcgacc gagctcttgc ggccgctggt cggcgccgac ccgaccctgg
     1381 cggcgctgtt cgtgatccag gccgaacagc tgctgcgcct ggcccagggc gattcgctga
     1441 ccgaccgggt ccgcggcgcg ctcgcggagc tgctacgcgg cgaggcgccc ggggtcgcgg
     1501 cggtcgctcg ccgcctcggg ctcagcgagc gcagcctgca gcggcgcctg cgagaggagg
     1561 gcacgagctt cgcggcgctg gtcgacgcga cccgtcagga gctggcgacc cactacctct
     1621 gcgacgccga cctgccggcg gccgaggtcg cgttcctgct cggcttcgcc ggcgcgccct
     1681 cgttctaccg cgcgtttcgc cggtggacgc agacgacgcc ggaggcctat cgcgcgggcc
     1741 ggcgcggcga ggcgcaggtg gctgtcccga ggggcaggtc ggtcacggtt actctgggcg
     1801 cttgacctcg cggtcgtgct gaggttcgat ccctgtcatg agcgcaaaga aaaccgagaa
     1861 agaaatgtgg accccttggg cttccaggca cccggaggct caaaacgtcg taatcacaga
     1921 actaacgacc agaggcttcg gttctgaact ccgagcaaga aaagtcttca aggattgtgg
     1981 ctacagcaaa gtccgcgcag atttttacca agatctggac tccgacaaga ccagggagat
     2041 cgatatcgtc gctcggttgc ctgttgaaga actgcatgat gcggaatgag cgaatttaac
     2101 tctatctgct cgcgctctga cgcgaaggct ccggtgctca catcgtcact gacgcggcac
     2161 gaaaagcacc ccgaagatgc ttggtatagc gctgccgtag cggtctttaa agccgtttgt
     2221 gacgcgggtg tcggggaggc tgaagcagca cgaaagagac gaggaaagca tgaggagaga
     2281 cctgacgtta aggtcgtcac ccctctgatc attcttgatg gaaatcttct gtgcgccgac
     2341 ctcggcgaac agggagagct tgaactgacg ccttgcgatc acattcagct ttccttcacg
     2401 ttctcaagca aggagtatgg ccggagcagg cttcactcac acatcgtgac gatggcagca
     2461 ggccgacatg gcggcgaacg ataacaagcc gccccggccg ccaggtgccg gcgggcggcg
     2521 gtcgcggtct cacggctcga actcggcgag ccgccacagc cctgcgggct cgtgcacctc
     2581 accgcgcgcg accaccagcc ggacgtcgcg gatcgcatgg atgtccgcg
//
LOCUS       DASWNJ010000943         3056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_509925, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000943 DASWNJ010000000
VERSION     DASWNJ010000943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509925"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VGB85_15990"
     CDS             <1..415
                     /locus_tag="VGB85_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135586.1"
                     /translation="LGVLAAHHTDIAFLGACDNLFLTADHLRLLFASLVAEPGLGGIL
                     PVDTPASGESTDRHFPHPLASAVRVAPAHAAAREVLGKGLQRPLFMFDRIGARWLDSA
                     SLPDRRVLRTCNTPQEYEAALAQAQADALPGSGPA"
     gene            429..1109
                     /locus_tag="VGB85_15995"
     CDS             429..1109
                     /locus_tag="VGB85_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HEY0135587.1"
                     /translation="MNDPATPPATDPTAPVPPAAPAVLAVARARSRNPIRRMYDWVLS
                     WAETPYGVPALCVLSFAESSFFPIPPDVLLIALALSVPAKAFRFAAWCTVASVLGGLF
                     GYFIGYALWTTFEPYLIPRVFSQENFSYVTGQYNEYSFLAVFIAAFTPVPYKVFTVAA
                     GVAQLNLIGFTLASIFGRGGRFFLVALVIRLLGPKARQWIDEYFNLATIIGTLLLIGG
                     FLLLKTLK"
     gene            complement(1160..>3056)
                     /locus_tag="VGB85_16000"
     CDS             complement(1160..>3056)
                     /locus_tag="VGB85_16000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY0135588.1"
                     /translation="DLALALDADTLDELERVPAPGRPRGLIADGDAVLVHGAQGFVVT
                     RVAGGAATTSSTGEDPRPAYVAAGQAFFTGAGDKFAADRSCNSCHADGLGDTLVWQAG
                     PFADARYATRTIQWLEGTGELGWTAYVTTVGNFAFGVTSNVGRRLDTEQQLGLSAYLA
                     SIMPPPPANGLTARDGTMSEQAQRGAALFAGAAGCVSCHPLPLTTSRALLPEGVTPGE
                     ADVPALVGAYRNGVWLKHGEATTMRAAVLASAEYSGVKLSDEQADDLTRYLAELTARD
                     FILLASEPDADSDIAPIDRPIRLTFSEPVWDDPENLAKIHLRGADGKDIAAKITVDGR
                     FVDLVATLAPQAAHSVVVDAGFESLRERPLDVAVTRDFTTAAAPKLVLDGEYTWTVQF
                     PAIDFAAQGFDFDTLLPTVATVTATPTASGADLAIDYGQGFVLATPVVISGTTLHWPA
                     LPIAVGGSGVDGHVTRVELVDVDADGVADEAVGEVEFSGPGMPDAPIAWDLRRPVVAG
                     ECEEGASGDVMVELTDIGATPTITWGDAQGLSLFVTDPQAVLPGGPGMPVTGGETQWS
                     IQLENFPDGFLGPVTYGVVPAGAVDTTEVSGGAGPGAAPLESGRCYKFSVLTTDFKSG
                     QRIVRLP"
BASE COUNT          381 a         1153 c         1064 g          458 t
ORIGIN      
        1 cctcggcgtg ctcgcggcgc accacacgga catcgcgttc ctcggcgcgt gcgacaacct
       61 gttcctgacc gccgaccacc tgcgcctgct gttcgcctcg ctggtggcgg agcccgggct
      121 cggcggcatc ttgccggtcg acacgccggc ctcgggcgag tcgacggacc gtcacttccc
      181 gcacccgctc gccagcgccg tccgcgtcgc tccggcgcac gcggccgcgc gcgaggtgct
      241 cggcaagggc ctgcagcggc cgctcttcat gttcgaccgg atcggcgcgc gttggctcga
      301 cagcgccagc ttgccggacc gccgcgtgct gcggacctgc aacaccccgc aggagtacga
      361 ggctgcgctc gcgcaggcgc aggccgacgc gcttcccggg tctggccccg cgtgatagct
      421 tcccggccgt gaacgacccg gcgacgccgc ccgcgaccga ccccacggcc cccgtgccac
      481 ctgctgcgcc cgctgtactc gctgtcgcac gcgcccgctc gcgcaacccg atccggcgca
      541 tgtacgactg ggtgttgtcg tgggccgaga ccccgtacgg cgtgccggcg ctgtgcgtgc
      601 tctcgttcgc cgagtcgagc ttcttcccga tcccgcccga tgtcctgctg atcgcgctgg
      661 cgctgtcggt cccggccaag gcgtttcgct tcgcggcgtg gtgcaccgtc gcgtcggtgc
      721 tcggcggcct gttcggctac ttcatcggct acgcgctgtg gacgaccttc gagccgtacc
      781 tgatcccgcg cgtgttctcg caggagaact ttagctacgt caccgggcag tacaacgagt
      841 acagcttcct cgcggtgttc atcgccgcgt tcacgccggt gccctacaag gtgttcacgg
      901 tcgccgccgg ggtcgcccag ctcaacctga tcggcttcac gctcgccagc atcttcggcc
      961 gcggcggccg cttcttcctc gtcgcgctcg tcattcgcct gctcggcccg aaggccaggc
     1021 agtggatcga cgagtacttc aacctcgcga cgatcatcgg caccctgctg ctgatcggcg
     1081 gcttcttgct gctcaagacc ctgaagtagc gccacgaccg tcgagccgct gcccgacggt
     1141 cgcccgcatc gtttcgctgc tacggcagac gaacgatccg ctggcccgac ttgaagtcgg
     1201 tcgtcagcac gctgaacttg tagcagcggc cggactcgag cggcgccgcg cccgggccgg
     1261 cgccgccgga cacctccgtc gtgtcgaccg cgcccgccgg caccacgccg taggtgacgg
     1321 gcccgaggaa gccgtccggg aagttctcga gctggatcga ccattgcgtc tcgccgccgg
     1381 tcaccggcat gcccgggccg cccggcagca ccgcctgcgg gtcggtgacg aacagcgaca
     1441 ggccctgcgc gtcgccccac gtgatcgtcg gcgtcgcgcc gatgtccgtc agctcgacca
     1501 tgacgtcgcc gctggccccc tcctcgcact cgccggcgac gacgggccgg cgcaggtccc
     1561 aggcgatcgg cgcgtcgggc atgccggggc ccgagaactc gacctccccg acggcctcgt
     1621 ccgcgacacc gtcggcgtcc acgtcgacga gctcgacccg cgtcacgtga ccgtcgaccc
     1681 cgctgccgcc gaccgcgatc ggcagcgcag gccagtgcag cgtcgtcccc gagatcacga
     1741 ccggcgtcgc cagcacgaag ccctgaccgt agtcgatcgc caggtccgcg cccgacgcgg
     1801 tcggcgtcgc ggtgacggtg gcgaccgtcg gcagcagcgt gtcgaagtcg aagccctgcg
     1861 cggcgaagtc gatcgccggg aactgcaccg tccacgtgta ctcgccgtcg agcacgagct
     1921 tcggcgcggc cgcggtcgtg aagtcccgcg tgaccgcgac gtcgagcggg cgctcgcgca
     1981 aggactcgaa gccggcgtcg acgacgacgc tgtgggcggc ctgcggcgcc agcgtggcga
     2041 cgaggtcgac gaagcggccg tcgacggtga tcttggcggc gatgtccttg ccgtcggcgc
     2101 cgcgcaggtg gatcttcgcg aggttctcgg ggtcgtccca caccggctcg ctgaaggtca
     2161 ggcggatcgg gcggtcgatc ggggcgatgt cgctgtcggc gtcgggctcg ctggcgagca
     2221 ggatgaagtc acgcgccgtc agctcggcga gatagcgggt caggtcgtcg gcctgctcgt
     2281 cgctgagctt cacgcccgag tactccgcgg acgcgagcac cgcggcgcgc atcgtcgtcg
     2341 cctcgccgtg cttgagccac acgccgttgc ggtaggcgcc gaccagcgcc ggcacgtcgg
     2401 cctcgcccgg cgtcacgccc tcgggcagca gcgcgcggct ggtcgtcagc ggcagcggat
     2461 ggcacgatac gcagccggcc gcgccggcga acagcgccgc gccgcgctgc gcctgctcgc
     2521 tcatcgtgcc gtcgcgcgcg gtcaggccgt tggccggcgg cggcggcatg atcgaggcca
     2581 ggtacgccga caggccgagc tgctgctcgg tgtcgaggcg ccgtccgacg ttgctcgtca
     2641 cgccgaacgc gaagttgccg accgtcgtca cgtacgcggt ccagccgagc tcgccggtgc
     2701 cctcgagcca ctggatcgtc ctcgtcgcat agcgagcgtc ggcgaacggg cccgcctgcc
     2761 acaccagcgt gtcgccgagg ccgtccgcgt ggcagctgtt gcacgagcgg tcggcggcga
     2821 acttgtcgcc ggcgccggtg aagaaggcct gaccggcggc gacatacgcc gggcgcgggt
     2881 cctcgccggt gctgctcgtc gtcgcggccc cgccggccac gcgcgtgacc acgaagccct
     2941 gcgcgccgtg cacgagcacc gcgtcaccat ccgcgatcag gccgcgcggg cggccgggcg
     3001 ccggcacgcg ctcgagctcg tcgagcgtgt cggcgtcgag ggcgagcgcc aggtcg
//
LOCUS       DASWNJ010000944         2417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_194578, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000944 DASWNJ010000000
VERSION     DASWNJ010000944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194578"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2417
                     /locus_tag="VGB85_16005"
     CDS             <1..>2417
                     /locus_tag="VGB85_16005"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0135589.1"
                     /translation="LSLGLARGLAAAHRQGVLHRDIKLANAMVSDRGDVKLLDFSLAK
                     LEAGAPALDQLRREALRRPTHPDGLIDQLDLTADLGNQATPPAVPPAPAVSPDTSRDA
                     SRDTAPNMLAVSRDASRDTAPNLPAVSPAEPDPDSGLDSTQVVRHPAVSPSVAFLRKF
                     EGGHRGVGADLTEAGTLVGTPHYMAPELWRAEPASRRSDIYALGVLMYFLCCDHPPTE
                     ARDALQLARRIQLREPRPLVELVPGIDPHFAAIVDRCIRRNPAERFASGEELRLALEA
                     LNPTTSRDGFVPAGNPYRGLRAFEAEHRELFFGRTAEIQTVVDRLRGQPFVLVAGDSG
                     VGKSSLCRAGVLPMITEGTIEPARTWFASSITPGRKPLRALVNALAGGLDIEEEELLA
                     QVRADPNALDRALRRQEGGARGRVVFIDQLEELVTLAPADDAAIVARMICQIAAGIPG
                     VRLLATARGDFLTRLAGLPGLADELTRALYFLPPLRAEGVREAIVGPAAAKHVHFESE
                     ALIEQLVTAGIEGSLPLLQFALAEIWEARPPGSDLITAAALDKLGGVSGALARHADGV
                     LARLSPDQRRAARLLLMRLVTLESTRASLTREELVGDDPNGRAALEALLAARLLVVRE
                     QPDGFVHELAHEALIQGWTTLQLWLEEEAEARVVRHRLEAAVTEWDRLGRPRDALWRE
                     RQLAELRLLDPATLRPRETDFVAASQARARRHRGLRNAAMLAAPVLLILAFVGVRLAQ
                     RAAIGEQVDAQLAGAAGIVAEARTRDAQLLARRKDAFARFDLGDSVGGEAAWELALGL
                     RREVEAL"
BASE COUNT          282 a          923 c          905 g          307 t
ORIGIN      
        1 ctcagcctcg gcctcgcgcg cgggctggcg gcggcgcacc ggcagggcgt gctgcaccgc
       61 gacatcaagc tcgccaacgc gatggtcagc gaccgcgggg acgtcaagct cctcgacttc
      121 agcctcgcca agctcgaggc cggggccccg gcgctcgacc agctgcgccg cgaagccttg
      181 cgccggccga cccaccccga cggcttgatc gaccagctcg acctgaccgc cgacctcggc
      241 aaccaggcga cgcccccggc ggtcccgccc gcgccggctg tctcgccgga cacctcgcgc
      301 gacgcctcgc gcgacacggc gccgaacatg ctcgccgtct cgcgcgatgc ctcccgcgat
      361 acggcgccga acctgcccgc cgtctcgccg gccgagcccg accccgacag cgggctcgac
      421 tcgacgcagg tggtgcggca cccggccgtc agcccgtcgg tcgcgttcct gcgcaagttc
      481 gagggcgggc accggggcgt cggcgccgac ctgacggagg cgggcacgct ggtcggcacg
      541 ccgcactaca tggcgcccga gctgtggcgt gccgagccgg cctcgcgccg ctcggacatc
      601 tatgcgctcg gcgtattgat gtacttcctg tgctgcgatc atccgccgac cgaggcgcgt
      661 gacgccttgc agctggcgcg ccggatccag ctgcgagagc cgcggccgct cgtcgagctg
      721 gtgccgggca tcgacccgca cttcgcggcg atcgtcgatc gttgcatccg ccgcaacccg
      781 gccgagcgct tcgcctccgg cgaggagctg cggctcgcgc tcgaggcgtt gaacccgacg
      841 acctcgcgcg acggcttcgt gccggcgggc aacccgtacc ggggcctgcg cgccttcgag
      901 gccgagcacc gcgagctgtt cttcggccgc accgccgaga tccagaccgt cgtcgatcgc
      961 ctgcgcggcc agcccttcgt gctcgtcgcc ggcgactcgg gcgtcggcaa gtcgtcgctg
     1021 tgtcgcgcgg gcgtgctgcc gatgatcacc gaggggacga tcgagccggc gcgcacgtgg
     1081 ttcgccagca gcataacgcc ggggcgcaag ccgctgcggg cgctggtcaa cgcgctcgcg
     1141 ggcggcctcg acatcgagga ggaggagctg ctcgcgcagg tgcgggccga cccgaacgcg
     1201 ctcgaccggg cgctgcggcg ccaggagggc ggcgcgcgcg ggcgcgtggt cttcatcgat
     1261 cagctcgagg agctggtcac gctggcgccg gcggacgacg ccgcgatcgt cgcgcggatg
     1321 atctgccaga tcgcggcggg gatccccggc gtgcggctgc tggcgaccgc gcgcggcgac
     1381 ttcctgaccc ggctggccgg gctgccgggg ctcgccgacg agctgacccg cgcgctgtac
     1441 ttcctcccgc cgctgcgcgc cgagggcgtg cgcgaggcga tcgtcgggcc ggcggcggcc
     1501 aagcacgtgc acttcgagtc cgaggcgctg atcgagcagc tggtcaccgc cggcatcgag
     1561 ggcagcctgc cgctgttgca gttcgcgctg gcggagatct gggaggcgcg gccgcccggc
     1621 agcgacctga tcacggcggc ggcgctcgac aagctcggcg gcgtctcggg ggcgctggcg
     1681 cggcacgccg acggcgtgct cgcccgcctg agcccggacc agcggcgagc cgcgcggctg
     1741 ctgctgatgc gcctcgtcac cctcgagtcg acgcgcgcga gcctgacgcg cgaggagctc
     1801 gtcggcgacg accccaacgg ccgggccgcg ctcgaggcgt tgctcgccgc ccgcctgctc
     1861 gtcgtgcgcg aacagccgga cggcttcgtg cacgagctcg cccacgaggc gctgatccag
     1921 ggctggacga ccctgcagct gtggctcgag gaggaggccg aggcccgcgt cgtgcgtcac
     1981 cgactcgagg cggcggtcac cgagtgggac cgcctcgggc gcccgcgcga cgcgctgtgg
     2041 cgcgagcgcc agctcgccga gctgcggctg ctcgacccgg cgacgctgcg gccgcgcgag
     2101 accgacttcg tggccgcgag ccaggcgcgg gcgcgtcgtc atcgcgggct gcgcaacgcg
     2161 gcgatgctcg ccgccccggt gctgctgatc ctcgcgttcg tcggcgtgcg cctggcccag
     2221 cgcgccgcga tcggtgagca ggtcgacgcc cagctcgcgg gcgccgcggg catcgtcgcc
     2281 gaggcccgca cccgcgacgc ccagctgctc gcgcgccgca aggacgcgtt cgcccgcttc
     2341 gacctcggcg actccgtcgg cggcgaggcg gcgtgggagc tcgcgctcgg gctgcgccgc
     2401 gaggtcgagg cgctgca
//
LOCUS       DASWNJ010000945         2226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_122679, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000945 DASWNJ010000000
VERSION     DASWNJ010000945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122679"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1578)
                     /locus_tag="VGB85_16010"
     CDS             complement(163..1578)
                     /locus_tag="VGB85_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135590.1"
                     /translation="MVRSTGVAARAGMCAVLVTAFASACAEEEPTSFCSEDTAVQLFR
                     DRRLDDLSTWTSGGLHFLGVLDVARINEKFLYLVERCDTPPLLIASGAHAVLPLFHVE
                     DVPPRSACLYDWSDGPYLVELDVTGERAPRRILDGFKCQAAQAASGVVLHKDGTWLFP
                     DFPDLGSARRILGVDAWPTDPGADALRYQTATGELHWFDISDATDTVVLSDVEAWWGR
                     GDQIFWRSSRPEDAEWVNLYDHRRRKSVRLGMYRPEDSAGPPYVAGQLSWRMNPPRTH
                     ALHIPFRAPDELEIFDLDGQPAPLPFTKPPFLWAPDGLVFAPGDLESEVQYARPGDLA
                     ARRLVYPPGASNQYGGLHGTNIEVFAGNSLWAVPLDGSPFREIARGVGRVEWLDDTHI
                     LTTFGGALITIDVATGARTVHAKDIYSLRPAGDGGYYFEIVARDPVTPDDPRVGIWYL
                     SATAALAPPGCEGLLGCPSRK"
     gene            complement(1648..>2226)
                     /locus_tag="VGB85_16015"
     CDS             complement(1648..>2226)
                     /locus_tag="VGB85_16015"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0135591.1"
                     /translation="AALALVMECASLLGRSEAGVASEDERRRLRALIPLAKLTTAKQA
                     VAMASEALECFGGAGYIEDTGLPRLLRDAQVLSIWEGTTNVLSLDVLRAEAKDGSFSA
                     VLKDMSRRAELLARPLDRNAVTAVRDAVRGLVGAAQTAVQAGGEALEASARKLALRTG
                     HVAEALYLAETAATNEVAAERFKRFAALRFGA"
BASE COUNT          371 a          811 c          731 g          313 t
ORIGIN      
        1 cggcaccctg gcgatcgcga tcgccagcgg accgaccctc ctcaataaca gcgtgatggc
       61 gatcccggcc gcgatctaca gcctgatcat gttcgtcacc gcggccgcgt tcggcttcat
      121 ggtcaatcga atgcaccgcg aggattgatc gaacggcccc gcctacttcc ttgacgggca
      181 gccgaggagg ccctcgcagc cgggcggcgc gagggcggcc gtcgcagaca ggtaccatat
      241 cccgacgcga gggtcgtcgg gagtgacagg gtcgcgggcg acgatctcga agtagtaccc
      301 gccgtcaccc gcaggtcgaa gcgagtagat gtccttcgca tgtacggtgc gcgcccccgt
      361 ggccacgtcg atcgtgatca gcgcgcctcc gaacgtcgtc aatatgtgcg tatcgtcgag
      421 ccattcgacg cgcccgaccc cgcgggcgat ctcgcggaac ggggagccgt cgaggggtac
      481 cgcccacagg gagttcccgg cgaacacctc gatgttcgtg ccgtggagcc cgccatattg
      541 attggatgcc ccgggcgggt agaccaggcg cctcgcagcg aggtcccccg gacgcgcgta
      601 ctgcacctcc gactcgaggt cccccggggc gaacaccagg ccgtccgggg cccacaagaa
      661 cgggggcttc gtgaacggca gcggggccgg ctgtccgtcg aggtcgaata tctccagctc
      721 gtccggagcc cgaaacggga tgtgcagcgc gtgagttcga ggcgggttca tccgccacga
      781 cagctgcccg gcgacgtagg gcgggccggc actatcttcc ggacgataca tcccgagcct
      841 gacgctcttt cggcgccgat ggtcgtaaag attgacccac tcggcgtcct ccggcctcga
      901 cgagcgccaa aagatctgat ccccgcggcc ccaccaggcc tcgacgtccg agaggacaac
      961 ggtgtcggtc gcgtcgctga tatcaaacca gtgcagctcc cccgtggcgg tctggtaacg
     1021 gagcgcatcg gcccctggat cggtgggcca ggcatcgacc ccgaggatcc gtcgtgcact
     1081 cccgagatca gggaagtccg ggaacagcca cgtgccatcc ttatgcagga cgacgccgct
     1141 cgccgcctgc gctgcctggc acttgaaacc gtccaggatg cgcctgggag cccgctcccc
     1201 cgtcacatcg agctcgacga gatacgggcc atcggaccag tcatagaggc acgcggatcg
     1261 cggaggaacg tcctcgacat gaaacagagg aaggacagca tgcgcgcccg aggcgatcag
     1321 caagggcggc gtgtcgcagc gctcgacgag atacaggaac ttctcgttga tccgggcgac
     1381 gtcgagcacg ccgaggaaat gcaagccgcc gctcgtccac gtcgacaggt cgtccaggcg
     1441 gcggtcacgg aacagctgca cggcggtgtc ctccgagcaa aagctcgtcg gctcctcctc
     1501 ggcgcaggcc gacgcgaacg ccgtcaccag aacggcgcac atgcccgctc gcgccgcgac
     1561 accggtcgag cggaccatgc caccatcgtt tcagacctcg cccggtctgt ccatacgcgc
     1621 ggcgacccgc agggcccgct gccgcgctca cgcgccgaag cgcagggccg cgaagcgctt
     1681 gaaccgctcg gccgcgacct cgttcgtcgc ggccgtctcc gcgaggtaca gcgcctccgc
     1741 gacgtgcccg gtcctcagcg cgagcttgcg ggcgctcgcc tcgagcgcct cgccaccggc
     1801 ctgcacggcc gtctgggccg cgccgaccag cccgcgcacc gcgtcacgca cggcggtcac
     1861 cgcgttgcgg tctagcgggc gagccagcag ctcggcccgc cgcgacatgt ccttgaggac
     1921 agccgagaac gacccgtcct tggcctccgc ccttagcacg tcgagcgaca gcacgttggt
     1981 cgtaccctcc cagatcgaca gcacctgcgc gtcgcgcagc agccgcggca gcccggtgtc
     2041 ctcgatgtag ccggcgccgc cgaagcactc gagcgcctcc gaggccatcg ccaccgcctg
     2101 cttcgcggtc gtcagcttcg ccagggggat cagcgcgcgc aggcgccggc gctcgtcctc
     2161 ggaggcgacg ccggcctcgc tgcggccgag caggctcgcg cactccatca ccagcgccag
     2221 cgcggc
//
LOCUS       DASWNJ010000946         2749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_400005, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000946 DASWNJ010000000
VERSION     DASWNJ010000946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400005"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="VGB85_16020"
     CDS             complement(<1..844)
                     /locus_tag="VGB85_16020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135592.1"
                     /translation="MRVRLGLSVGLLLGCGCQDVQESSACEEPAPVTAQRVEPPTPVT
                     PESAPEPAAPPAVDRGLAPLDAEAQALLAAGAEERIISRREHFTVSNEYRHDLWSPYL
                     RGLGGVHVGVASDQNYTLMAVAGSELGYLIDLDRDVVRLHRVYMTLITASPDPETFLG
                     RLDAAAQRETAALIEAGLADMPADDRAAAQNFVVDHREALRTYLGEVRAPGEHHAGTW
                     LSDPELYEHMREMVAGGRLRPINGNLAGKTAMKTIAAGARALGLPVKTFYLSNAEEYL
                     SYTAA"
     gene            953..1483
                     /locus_tag="VGB85_16025"
     CDS             953..1483
                     /locus_tag="VGB85_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135593.1"
                     /translation="MNRKLLRGEYPVATWMGVTLTTHRIWQVVREGSTSAMVSFPVES
                     VQWTRLGRSHLPYLLWMALIVAVLGVVALEHDRTVTYWLFGVVLLLLLLYATSRRLML
                     SIGAGHGEIVVQVAGEGASVEAALEFIHQVETRRLTTTAPERATTSASGSSSSWVSPP
                     DPAEPMTDLNSVTMGV"
     gene            1610..>2749
                     /locus_tag="VGB85_16030"
     CDS             1610..>2749
                     /locus_tag="VGB85_16030"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0135594.1"
                     /translation="MATTQWTLPEQFDEYRLVRMLGRGSMGVVFLGHDLVLDRPVAIK
                     FLNAVDANTRARERFLTEARAAARIQHPNVMGIYRVGELEGRLYIIGEYIRGESLNEL
                     PRPMPWQEVLKLAVGLARGLACAHRQGVLHRDIKLANAMRSESGEVKLLDFSLAKLLE
                     GGPMSGPMPAASTSAGRIPSDPEKQDPEATEQLRLVIGKNAKGRRADPAPGVPVPAAR
                     EGVALDSTVARAPARPSVLPPPPPPPNINDSSDSSVVGTPYYMAPELWRSQPASRGSD
                     VYALGVLLYILCYGMPPIEGSTTIELARRSQEVVPKPLRSVAPAVDARFAEIVDRCLR
                     PPAERYPSADELCTALETLALGAGPAEVVRGNPYRGLQAFEARHRA"
BASE COUNT          390 a         1044 c          911 g          404 t
ORIGIN      
        1 acgccgccgt gtacgacaga tactcctcgg cgttgctgag atagaacgtc ttgaccggca
       61 gcccgagcgc gcgcgccccg gcggcgatcg tcttcatggc tgtctttccg gccaggttgc
      121 cgttgatcgg ccgcaggcgc ccgcccgcga ccatctcacg catgtgctcg tacagctcgg
      181 ggtccgacag ccaggtcccc gcgtggtgct cgccgggggc ccgcacctcg ccgaggtacg
      241 tacgcagcgc ctcgcggtgg tcgacgacga agttctgcgc ggcggcccgg tcgtccgccg
      301 gcatgtcggc gagcccggcc tcgatcagcg ccgccgtctc cctctgcgcc gcggcgtcga
      361 ggcgcccgag gaaggtctct ggatcgggcg acgcggtgat cagcgtcatg tacacgcgat
      421 gcagccggac gacgtcgcga tcgaggtcga tcaggtagcc gagctcgctg ccggcgaccg
      481 ccatcagcgt gtagttctgg tccgacgcca cgcccacgtg gaccccgccg agcccgcgca
      541 ggtacgggga ccacaggtcg tggcggtact cgttgctgac cgtgaagtgc tcgcgccggc
      601 tgatgatccg ctcctcggcg cccgccgcga gcagggcctg cgcctccgca tcgagcggcg
      661 ccaggccgcg atcgaccgcg ggcggagctg ccggctccgg cgcactctca ggagtcaccg
      721 gagtcggcgg ctcgacgcgc tgcgccgtga ccggcgccgg ctcctcgcac gcggacgact
      781 cctgaacgtc ctgacagcca cagcccagca acaacccgac actcaacccc agccttaccc
      841 gcatcgcgac gcgacgatac tgcaactccc cgcgcggcgg cggcccctgg ggcgtgcgcg
      901 atgccgctac ccgggctcgc cgcggctcgg gtagcatggc tgggaggcac gcatgaaccg
      961 caagctgctc cgtggcgagt accccgtggc gacctggatg ggcgtcaccc tgaccaccca
     1021 ccgcatctgg caggtcgtgc gcgagggttc gacctccgcg atggtgtcgt tcccggtcga
     1081 gtccgtgcag tggacgcggc tcggtcgcag tcatctgccg tatctgttgt ggatggcgct
     1141 gatcgtcgcc gtgctaggcg tcgtcgcgct cgagcacgac cgcaccgtca cctactggtt
     1201 attcggcgtc gtgttgctgc tgctcctgtt gtatgcgacc tcgcgacggc tgatgctctc
     1261 gatcggcgcc ggccacgggg agatcgtcgt gcaggtcgcg ggcgagggcg cgagcgtcga
     1321 ggctgcgctc gaattcattc atcaggtcga gacgcgacgc ctcaccacca ccgcgccgga
     1381 gcgagcgacc accagcgcga gcggctcctc gagctcctgg gtcagcccgc ccgaccccgc
     1441 cgagccgatg accgacctca actccgtgac gatgggcgtg taactgtccc catcgtacgc
     1501 ataagttgca gacgctcgaa ggatcggcgc gcgcgggcca tgaagtaggt tcgcaagcct
     1561 ggcacgagcg ccctgtacgc gcgtgcctcc ccggtcccct catctggtca tggcgacgac
     1621 gcaatggacg ctgcccgagc agttcgatga gtaccgcctg gtgcggatgc tcgggcgcgg
     1681 cagcatgggc gtggtcttcc tcggccacga cctcgtgctc gaccgcccgg tcgcgatcaa
     1741 gttcctcaac gccgtcgacg ccaacacccg cgcccgcgag cgcttcctga ccgaggcgcg
     1801 cgccgccgcg cgcatccagc acccgaacgt gatgggcatc tatcgcgtcg gcgagctcga
     1861 gggtcgcctc tacatcatcg gcgagtacat ccgcggcgag agcctcaacg agctgccgcg
     1921 cccgatgccc tggcaggagg tcctcaagct cgccgtcggc ctcgcccgcg gtctcgcctg
     1981 cgcgcaccgc cagggtgtcc tccatcgcga catcaagctc gccaacgcga tgcgcagcga
     2041 gtccggcgag gtcaagctcc tcgacttcag cctcgccaag ctgctcgagg gcggcccgat
     2101 gtccgggccg atgcccgccg cctcgaccag cgccggccgg atccccagcg acccggagaa
     2161 acaggacccc gaggcgacgg agcagctgcg cctcgtcatc ggtaaaaacg ccaagggtcg
     2221 ccgcgccgac cctgcgccgg gcgtgcccgt gcccgccgcg cgcgagggcg tcgcgctcga
     2281 cagcaccgtc gcccgcgcgc cggcccggcc atcggtgttg ccaccaccac caccgccacc
     2341 gaacatcaac gactcgagcg actcgagcgt cgtcggcacc ccgtattaca tggcgcccga
     2401 gctgtggcgc tcgcagccgg cctcgcgcgg ctccgacgtc tacgcgctcg gcgtgctgct
     2461 ctacatcctg tgttacggca tgccgccgat cgaaggcagc accacgatcg agctcgcgcg
     2521 acgcagccag gaggtcgtcc cgaagccgct gcggtcggtc gcgcccgccg tcgacgctcg
     2581 cttcgccgag atcgtcgacc gctgcctgcg gccgccggcg gagcgctacc cctcggccga
     2641 cgagctgtgc accgcgctcg agaccctcgc gctcggcgcg ggcccggccg aggtcgtgcg
     2701 cggcaacccc taccgcggcc tccaggcctt cgaggcccgc caccgcgcc
//
LOCUS       DASWNJ010000947         5191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_194751, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000947 DASWNJ010000000
VERSION     DASWNJ010000947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194751"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VGB85_16035"
     CDS             <1..655
                     /locus_tag="VGB85_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135595.1"
                     /translation="AAALASAGERLEAETAAFASARSAVVQLRQRALVERRQVVAEQG
                     QYNARTLAQWRVAGTDDRARRLALFTGVVAGSEAMYEVRDQGLEWEESVLAARTALAI
                     DGAALHRFVRQLLGLSRPLRFTDGVRFYVEYGAQVAAQVDADLAEVQASVAAAQEAVR
                     PPPPGSPDAGPAAKLPLNQPPTQPPPTEPSKPPEQPKPIRDGQLRRDPITGVPTPPP"
     gene            666..1082
                     /locus_tag="VGB85_16040"
     CDS             666..1082
                     /locus_tag="VGB85_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135596.1"
                     /translation="MPNKRPPPVRQPSPTRREPGALEVVAAAIDGLADAGQLLRTRLD
                     AIDPLEHAARWAGINDEIRALDDRIHRLREEQQRLALAGTELRPLATGELERLRQATA
                     TVAELVRTTGRIAAVAEAAVRLSDAAGAVIQRLQRI"
     gene            complement(1116..3050)
                     /locus_tag="VGB85_16045"
     CDS             complement(1116..3050)
                     /locus_tag="VGB85_16045"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HEY0135597.1"
                     /translation="MHQSTVGPLAVVIALSLGTGCGDSTQIDTVGSTGGASTGPASAT
                     EPTSSSEPTSTSAPTTESPGTTNAPTSEGTTGTISDPGTTDPTSEGTTGDDSTGTTGE
                     PPDPAEPFFEPGVIAELALVLSAEAMDSLDADPKIYVSGDLTVTLGDQVIVLTDIGVR
                     LKGNYGSYRTLDEKAAFLLNFDRYVDDQRLFDMEKLAVNNMVQDPSMQREQLGYALFR
                     AGDVPAPRAGHATVSVNGEPYGLYTTVETVDNESFLKHWFDEDNGNLYEGAYGSDITT
                     ELVPSFDQDNGDNVDFMDLQALAAALDAITDPADFVAEASKVIDLDRFLSFAATEIYL
                     GHWDGYAWTRNNFFVYRGPDQRWVFFPWGIDQTMVDNLGPFGGQGRIQQMCTESLECR
                     MMLAAKFEEVVARVDELGLAAQAQTLADAVHDAAAADPRKEYSIDQVDGTVAANIEFL
                     TNRGGTVKDDLICTDPSQVDADMDGFSGCGEDCNDGDKAIHPGAVEVCDLDDDNCDGQ
                     WDNDPKCPQCILKKLPAPAVGNAAFCFGEKTWADAEADCVTQKGHLIAIHSQAVQDFL
                     SAQAFPIQDTDWWIGLSDSKNEGAFVWSNGSKVDYTSWAGGEPNNAGNEDCTNLAPWT
                     GGDWNDLFCEQARPYICAVP"
     gene            3194..3385
                     /locus_tag="VGB85_16050"
     CDS             3194..3385
                     /locus_tag="VGB85_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135598.1"
                     /translation="MAGEDPSMVCPRCGSRAVEVSPGRASPCMPLPPVEGLRCTACGN
                     TGTRMRLRERTLVDWRHEG"
     gene            3417..4448
                     /locus_tag="VGB85_16055"
     CDS             3417..4448
                     /locus_tag="VGB85_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135599.1"
                     /translation="MRTSDGPWILGARTRRLALLMLAAGGPACKAGGGDEAGAGDATT
                     MTMMMTTTTGAPTTGDASETAGTSGTPTTAGPPDLPSPRACNGDAALCERRFDEVVFP
                     CTHNAFAARDDGFAQINANQQHGVAQQLADGVRCMMLDVQDLDGETALCHGGCALGKL
                     DHLEVLRDIEGFLADNPDEVLTIIYQDDIASDRIVADLEASGLSQRVYTRTPGEPWPT
                     LAAMIDADTRLLVTAENGGPPPAWFHHVWDLTWDTPYTFHSIEEFSCALNRGEIGNEL
                     FLVNHWVSTMFDTPSQPDAKIANAYDVLHARAVQCQQEARQLPNFLAVDFYEQGDLFA
                     AVRALNGLQ"
     gene            complement(4457..4720)
                     /gene="csrA"
                     /locus_tag="VGB85_16060"
     CDS             complement(4457..4720)
                     /gene="csrA"
                     /locus_tag="VGB85_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HEY0135600.1"
                     /translation="MLTLTRKVGQKIRIGDHIEIVVREIRGRQVRLGISAPQGLPVYR
                     EELYQQIAQEGGVPKAGEVGEAGEAGEISAEVEIEAAETDDER"
     gene            complement(4922..>5191)
                     /locus_tag="VGB85_16065"
     CDS             complement(4922..>5191)
                     /locus_tag="VGB85_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135601.1"
                     /translation="RLGELYLQLGQPARAVPLLRLAGADDPEQANIAASEGRALLLTN
                     ERAAARAALTRALRVNPFIPTLHCDLARLAVSDEERSREQALCRE"
BASE COUNT          725 a         1818 c         1875 g          773 t
ORIGIN      
        1 ggcggccgcg ctggcgagcg cgggcgagcg cctcgaggcc gagaccgccg cgttcgcctc
       61 ggcccgcagc gcggtcgtcc agctgcgcca gcgcgcgctc gtcgagcgcc gccaggtggt
      121 cgccgagcag ggccagtaca acgcgcgcac gctggcgcag tggcgggtcg ccggcaccga
      181 cgaccgggcc cggcgcctcg cgctgttcac cggcgtggtc gccggtagcg aggcgatgta
      241 cgaggtgcgc gaccagggcc tcgagtggga ggagagcgtg ctcgcggcgc ggacggccct
      301 ggcgatcgac ggcgccgcgt tgcaccgctt cgtgcgtcag ctgctcgggc tgtcgcgacc
      361 gctgcgcttc accgatggcg tgcgcttcta cgtcgagtac ggcgcccagg tggccgcgca
      421 ggtcgacgcc gacctcgcgg aggtgcaggc gagcgtcgcc gcggcgcagg aggccgtccg
      481 cccgccgccg ccgggcagcc ccgacgccgg cccggctgcc aagctcccgc taaaccagcc
      541 gcctacccag ccgccgccga ccgagcccag caagcctccc gagcaaccaa agccgatccg
      601 cgacggccag ctgcgccgcg acccgatcac cggcgtcccg accccgccgc cctgaggagc
      661 tctcgatgcc gaacaagcgc cccccacccg tcaggcagcc cagccccacg cgtcgcgagc
      721 ccggcgcgct cgaggtggtc gccgccgcga tcgacggcct cgcggacgcc ggccagctgc
      781 tgcgcacgcg cctcgacgcg atcgatccgc tcgagcacgc ggcccgctgg gcgggcatca
      841 acgacgagat ccgggcgctc gacgatcgca tccaccgcct gcgcgaggag cagcagcgcc
      901 tcgcgctcgc gggcaccgag ctgcgcccgc tggcgaccgg ggagctcgag cggctgcgcc
      961 aggcgaccgc gaccgtcgcc gagctggtgc gcacgaccgg gcggatcgcc gcggtcgccg
     1021 aggccgcggt gcgcctctcg gacgcggcgg gggccgtgat ccagcggctc cagcgcatct
     1081 agcccatcag gccgccgggt cccggctgac gcggctcagg gcaccgcgca gatgtacggc
     1141 cgggcctgct cgcagaacag gtcgttccag tcgccgccgg tccacggcgc gaggttggtg
     1201 cagtcctcgt tgccggcgtt gttgggctcg ccgccggccc agctcgtgta gtcgaccttg
     1261 ctcccgttgc tccacacgaa ggcgccctcg ttcttgctgt cgctcaggcc gatccaccag
     1321 tcggtgtcct ggatcgggaa cgcctgcgcg gacaggaagt cctgcaccgc ctggctgtgg
     1381 atcgctatca ggtggccctt ttgcgtcacg cagtcggcct cggcgtcggc ccaggtcttc
     1441 tcgccgaagc agaacgccgc gttgccgacc gccggcgcgg gcagcttctt gaggatgcac
     1501 tgcgggcact tcgggtcgtt gtcccactgg ccgtcgcagt tgtcgtcgtc gaggtcgcac
     1561 acctcgacgg cgccgggatg gatcgccttg tcgccgtcgt tgcagtcctc gccgcagccg
     1621 ctaaagccgt ccatgtcggc gtcgacctgg ctcgggtcgg tgcagatcag gtcgtccttc
     1681 accgtgccgc cgcggttggt caggaactcg atgttggccg cgacggtgcc gtcgacctga
     1741 tcgatcgagt actccttgcg cggatcggcg gcggcggcgt cgtggaccgc gtccgcgagg
     1801 gtctgcgcct gcgcggcgag gccgagctcg tcgacgcgcg cgacgacctc ctcgaacttc
     1861 gccgcgagca tcatgcggca ctcgagcgac tcggtgcaca tctgctggat ccggccctgg
     1921 ccgccgaacg gccccaggtt gtcgaccatc gtctgatcga tgccccacgg gaagaacacc
     1981 cagcgctgat ccgggccgcg gtacacgaag aagttgttgc gcgtccacgc gtacccgtcc
     2041 cagtgaccga ggtagatctc ggtggcagcg aagctcagga agcggtcgag gtcgatcacc
     2101 ttgctcgcct cggcgacgaa gtcggccggg tcggtgatcg cgtcgagcgc ggccgcgagc
     2161 gcctgcagat ccatgaagtc gacgttgtcg ccgttgtcct ggtcgaagct cggcaccagc
     2221 tcggtcgtga tgtcgctgcc gtaggcgccc tcgtacaggt tgccgttgtc ctcgtcgaac
     2281 cagtgcttga ggaacgactc gttgtcgacc gtctcgaccg tcgtgtacag gccgtagggc
     2341 tcgccgttga ccgacaccgt cgcgtggccg gcccgcggcg cgggcacgtc gccggcgcgg
     2401 aacagggcgt agccgagctg ctcgcgctgc atgctcgggt cctggaccat gttgttgacc
     2461 gcgagcttct ccatgtcgaa caatcgctgg tcgtcgacgt agcggtcgaa gttgagcaag
     2521 aacgcggcct tctcgtcgag cgtgcgatac gagccgtagt tgcccttgag ccgcacgccg
     2581 atgtcggtga gcacgatgac ctgatccccg agcgtcacgg tgaggtcgcc ggacacgtag
     2641 atcttggggt cggcgtcgag gctgtccatc gcctccgcgc tcagcacgag cgcgagctcg
     2701 gcgatcacgc ccggctcgaa gaagggctcc gccggatccg gcggctcgcc ggtcgtgccc
     2761 gtgctgtcgt cgccggtggt gccctcgctc gtcgggtcgg tcgtgccggg atcgctgatc
     2821 gtgccggtgg tgccctcgct cgtcggggcg ttcgtcgtgc cgggcgactc cgtggtcggc
     2881 gcgctggtcg aggtcggctc gctcgacgag gtcggctccg tggcgctcgc gggacccgtg
     2941 ctcgcgccgc cggtggaccc gacggtgtcg atctgcgtcg agtctccgca gcctgtcccg
     3001 agggacaggg caatgacgac agcgagagga ccgacggtag attgatgcat gggcgctcca
     3061 gggaacaagg gaccactaca gcggcggcgc gcctcgctgt caagccggac ggtcgcggag
     3121 agtccgcccg cagcgccgtg gagggacgct cgacggaccc cgcatgacga cctgtcgatg
     3181 gtagcgtgcg gcgatggccg gcgaggaccc gagcatggtg tgcccgcgtt gcgggtcacg
     3241 ggcggtcgag gtgtcgccgg gccgcgcgag cccctgcatg ccgctgccgc cggtcgaggg
     3301 cctgcgctgc acggcctgcg gcaacaccgg cacgcgaatg cgcctgcgcg agcgcacgct
     3361 cgtcgactgg cggcatgagg ggtgacacgc gggcgtggac gggcgatcct gtaccgatgc
     3421 gaacaagcga cggcccctgg atcctcggcg cgagaacgcg tcggctcgcg ttgctgatgc
     3481 tcgcggcggg cgggccggct tgcaaggccg ggggcgggga cgaggccggc gctggcgatg
     3541 ccacgaccat gacgatgatg atgacgacga cgacgggcgc tccgacgacg ggcgacgcga
     3601 gcgagaccgc gggcaccagc ggcacgccga cgaccgcggg cccgcccgac ctgccgtcgc
     3661 cgcgcgcgtg caacggcgac gccgcgctgt gtgagcgccg cttcgatgag gtcgtgttcc
     3721 cgtgcacgca caacgccttc gccgcgcgcg acgacggctt cgcccagatc aacgccaatc
     3781 agcagcacgg cgtggcgcag cagctggcgg acggcgtgcg ctgcatgatg ctcgacgtgc
     3841 aggacctcga cggcgagacg gcgctgtgtc acggcggctg cgcgctcggc aagctcgacc
     3901 acctcgaggt gctgcgcgac atcgagggct tcctcgcgga caaccccgac gaggtgctga
     3961 cgatcatcta tcaggacgac atcgcgtcgg accgcatcgt cgccgacctc gaggcgagcg
     4021 ggctgtcaca gcgggtctac acgcgcacgc ccggcgagcc gtggccgacg ctggcggcga
     4081 tgatcgacgc cgacacgcgg ctgctggtca ccgccgagaa cggcggaccg ccgcccgcgt
     4141 ggttccatca cgtgtgggac ctgacctggg acacgccgta cacgttccac tcgatcgagg
     4201 agtttagctg cgcgctcaac cgtggggaga tcggcaacga gctgttcctc gtcaaccact
     4261 gggtgagcac gatgttcgac acgccgtcgc agccggacgc caagatcgcc aacgcgtacg
     4321 acgtgttgca cgcccgcgcg gtgcagtgcc agcaggaggc caggcagctg ccgaacttcc
     4381 tcgcggtcga cttttatgag cagggcgacc tgttcgcggc ggtgcgggcg ctcaacgggc
     4441 tgcagtgagc ccggggctag cgctcgtcgt cggtctcggc ggcctcgatc tcgacctcgg
     4501 cgctgatctc accggcctca ccggcctcgc cgacctcgcc cgccttgggg acgccgccct
     4561 cctgggcgat ctgctggtag agctcctcgc ggtacacggg cagcccctgg ggcgccgaga
     4621 tcccgagacg gacctgacgg ccgcggatct cgcggacgac gatctcgatg tggtcaccga
     4681 tgcggatctt ttgtccgact tttctggtta gcgtcaacat caggcgcctc cgtggcagta
     4741 cgggtgcaat ggccaggacg atggactgag agcggcagaa gaggcctgag cctgctgcca
     4801 gggccctcta gctattcgtg aagacggtgt tttgcgcaag ccataaaatt ggccccaatg
     4861 cgcgcgtacg cgcgcggaat caaccaccgg gcgccagggc ggacctgcac tttgggacag
     4921 gtcactcgcg acacagcgcc tgttcgcgac tgcgctcctc gtcgctgacg gcgaggcgcg
     4981 cgagatcgca atgcagggtc gggatgaagg ggttgacgcg cagcgcccgg gtcagcgcgg
     5041 cgcgggccgc cgcgcgctcg ttggtcagga gcagcgcgcg cccctccgag gcggcgatgt
     5101 tggcctgttc ggggtcgtcg gcgccggcga ggcgcaggag cggcacggcc ctggccggct
     5161 gaccgagctg caggtacagc tcgccgaggc g
//
LOCUS       DASWNJ010000948         1657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_687850, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000948 DASWNJ010000000
VERSION     DASWNJ010000948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687850"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VGB85_16070"
     CDS             complement(<1..678)
                     /locus_tag="VGB85_16070"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEY0135602.1"
                     /translation="MSTAQRIVLVIGQLLVLAVFVWLFLPGAPGRTTLGRPLVALEWA
                     EAPRNMRTEPFRDAHGVVLRNDSAYTVPNVPMARVRGPFTITLEARIDRPANAQLPLV
                     SRWLPENGGRSFQIGLQKNLRPYLTVSSNGRKDDPAEFAELNTKVAVPTNKPFHLAAV
                     FDPDERMALFLDGKLIGELVGDDRVPSSAYASSAPLTLGLAKVPLHGLFGPLYIFDRV
                     LADEAIAA"
     gene            complement(678..>1657)
                     /locus_tag="VGB85_16075"
     CDS             complement(678..>1657)
                     /locus_tag="VGB85_16075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HEY0135603.1"
                     /translation="QRYLLRLKDRGIILAVASKNQAEAARLPFEKHPEMVLRPDDIAI
                     FRANWVDKATNIREIAETLNIGLDSLVFIDDNPVERALVRGELPMVEVPEVGDDAATY
                     RETLDRGLFFETVTFSRDDRMRAEAYRMNAKRAEEAASATDLTSFLQGLAMTGTVGRL
                     DDFNLPRFAQLLNKSNQFHLTTTRYTEAQLRAIMADPQRVCLYFRLEDRFGDNGLIGT
                     IVLDLPKDPADPLDHLRIDTWAMSCRVLSRGMEAFIMSEIRRHAGSARIIGHYIPTAK
                     NSLVKGLYERLGFRKIDDINGTTVWELRPEDEVDYSNHIKRVEATAAGS"
BASE COUNT          244 a          490 c          648 g          275 t
ORIGIN      
        1 ggccgcgatc gcctcgtcgg cgagcacgcg gtcgaagatg tagagcgggc cgaacagacc
       61 gtgcagaggg accttggcga ggccgagggt cagcggcgcg gacgaggcgt aggcgctcga
      121 cgggaccctg tcgtcgccga cgagctcacc gatgagcttg ccgtcgagga acagggccat
      181 acgctcgtcg gggtcgaaca cggcggcgag gtggaacggc ttgttggtcg gcacggcgac
      241 cttggtgttg agctcggcga actcggcggg gtcgtccttg cggccgttgc tcgacacggt
      301 cagatacggg cgcaggttct tttgcaggcc gatctgaaag ctgcgcccgc cgttctcggg
      361 cagccagcgc gacacgagcg ggagctgggc gttggccggg cggtcgatgc gggcctcgag
      421 cgtgatcgtg aaggggccgc gtacgcgcgc catcggcacg ttcggcacgg tgtacgccga
      481 gtcgttgcgc aggacgacgc cgtgggcgtc gcgaaacggc tcggtccgca tgttgcgggg
      541 cgcctcggcc cactcgagcg cgacgagcgg gcggccgagg gtggtccgac cgggcgcgcc
      601 gggcaggaac agccacacga acaccgcgag gaccagcagc tggccgatca cgagcacgat
      661 gcgctgggcg gtcgacatca tgatccggcg gccgtcgcct cgacgcgctt gatgtggttg
      721 ctgtagtcga cctcgtcctc gggtcgcagc tcccacacgg tcgtgccgtt gatgtcgtcg
      781 atcttgcgga agccgaggcg ctcgtagagg cccttgacga gcgagttctt ggcggtcggg
      841 atgtagtggc cgatgatgcg ggcgctgccg gcgtggcgcc ggatctcgga catgatgaag
      901 gcctccatgc cgcgcgagag gacccggcag ctcatcgccc aggtgtcgat gcgcaggtgg
      961 tcgagcgggt cggcggggtc ctttggtaga tcgagcacga tcgtgccgat gaggccgttg
     1021 tcgccgaagc ggtcctcgag gcgaaagtac aggcagacgc gctgcgggtc ggccatgatc
     1081 gcgcgcagct gggcctcggt gtagcgcgtg gtcgtcaggt ggaactggtt gctcttgttg
     1141 agcagctggg cgaagcgcgg caggttgaag tcgtcgaggc ggccgacggt gccggtcatc
     1201 gcgaggccct gcaggaagct cgtcaggtcg gtcgcggacg cggcctcctc ggcgcgcttg
     1261 gcgttcatgc gatacgcctc ggcgcgcatg cggtcgtcgc gggagaaggt gacggtctcg
     1321 aagaacaggc cgcggtcgag ggtctcgcgg taggtggccg cgtcgtcgcc gacctcgggg
     1381 acctcgacca tcggcagctc gcctcgaacc agcgcgcgct cgacggggtt gtcgtcgatg
     1441 aacacgagcg agtcgaggcc gatgttgagg gtctcggcga tctcgcggat gttggtcgcc
     1501 ttgtcgaccc agttggcgcg aaagatcgca atgtcgtccg ggcgcaggac catctctggg
     1561 tgcttttcga agggcaggcg ggcggcctcg gcctggttct tgctggcgac cgcgaggatg
     1621 atgccgcggt ccttgaggcg cagcagatag cgctgga
//
LOCUS       DASWNJ010000949        10141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_653730, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000949 DASWNJ010000000
VERSION     DASWNJ010000949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 10141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10141
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653730"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1698
                     /locus_tag="VGB85_16080"
     CDS             <1..1698
                     /locus_tag="VGB85_16080"
                     /inference="COORDINATES: protein motif:HMM:NF024983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0135604.1"
                     /translation="RDPPFSKLDLILCRNVLIYLGTVLQRKLMNVFHYALKPSGYLML
                     GAAETVGGQGELFAVVDKKHRIFAKKLVERSIYADLPIEYRAGRGPTPGPRVTSEPVP
                     ASDMQSEVNRLILDRYGPPGVVVDAQFQIIQFRGRTGDFLEPAPGDAAQSLNVLKMAR
                     EGLLHGLQAALFNARKTEAPVRKEGLRVHSGGHVREIHLQVVPLAAPGEARHYLVLFE
                     DVRAGDDDRPPPPPPLAVDDHLSQIQDELAASREYLQSIIQDLEAANEELQSANEEIL
                     SSNEELQSTNEELDTAKEELQSTNEELNTVNEELHGRNEELSRVNSDLINLLGSVEIA
                     IVIVGADLAIRRFTPMAEKILNLIPTDLGRPISQIKPNIDCPELERMITHVIATIEIQ
                     EQNVRDRDGNWYTLRIRPYKNVDNRIDGAVLALFNIAGRRHELELRQAHDNIAAIIET
                     AHEPMIVLDGHLRVHFLNHAFSRRFAVARERALERQIFDLADGAWNVPQLRHLLAGLT
                     PGRNRVEAVRLTHDFGAATPRHVRINASCIPGFEPSASFVVLAITDAEPDAGMGAGT"
     gene            1715..3418
                     /locus_tag="VGB85_16085"
     CDS             1715..3418
                     /locus_tag="VGB85_16085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0135605.1"
                     /translation="MTDADEKARLLAENAQLRHTLARQEAEHFAERTTLRIELETLDH
                     QRHYLSESVDWYAELYEFAPIAYLALDATGLIRDINLTGTALLGTERQNLLGRPMRLH
                     VVSEDRRIFLEHIYRSRRTNGAIVSELRILAAEGRELPAQLISRRGFGGDGPVTLRTA
                     IFDLSERKRAEADLLLGHRRLSLALAVSQAGLHEYVVGRPGMQSSARWLEILGLPPVD
                     ADDAALRAWFEGRIEPADAEARAQALAAFLAGTTPTHVAEFRVQRHDDAWIWVRELAD
                     AAQRTPDGRPSRVVGVLLDISDEKRRLAEAEQRTAQLRELSAALYQVEENERRELATL
                     LHDDLGQQLVAAQLQLSALGRKLDGPPPASLGLVVKMLREAQDTIRSLSFQLSPPILH
                     ELGLIAALRWLAGELERTLELAVSIEADEPLPPLRGDPEVVLFRCVRELLLNVAKHAG
                     VSEVRVVVRDVPDSPYCQILVEDHGVGFAADRLTRERSKARSFGLLSVYERIQGLGGR
                     VLVTSAPGQGTRALLMLPRDDDLDDDEEVSEVDGVDEVDGVDGVDGVDEVDGVLDPLE
                     D"
     gene            3754..4878
                     /locus_tag="VGB85_16090"
     CDS             3754..4878
                     /locus_tag="VGB85_16090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135606.1"
                     /translation="MLTRHTWTLLLVTACQGSTANQTGFGSGPEPTTTPLASTGEGST
                     SSSSTGTSTSEGSGSGSSAADTSAEPPRDLGVMPDFGNGAPIGCQGKIDLLFVISRHG
                     NMLYRQEQLALAFPQFVAAIEAKFTNFDYHIMVVTGDPGWGNDTCTQVCPTPTCKIGE
                     DCCPLSPQNGDTGQPCCHMPDYPCQDLDLVTPCDRTWGAGEVFPAGTATQNKPCAIDG
                     DRRYLVKGQTDLAETFECIATVGASGYDLLGQALTAAMQKPINDPGGCNNGFLRDDAL
                     LMVTFISTTADTPTDSEGTPAEWAKAVLDAKHGDDRSVVAFDIGGGGPECLYYDRICH
                     MTEAFPYHHHVYVEEADYGPGFLAAASLVETACAGFVVPG"
     gene            complement(4888..6459)
                     /locus_tag="VGB85_16095"
     CDS             complement(4888..6459)
                     /locus_tag="VGB85_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135607.1"
                     /translation="MVCEDGCSTSTAQPGSTGDQTPTTGDSADTVTGDSVDHSSGSTA
                     PDAEGGTDTIDTTGEPAELPLIVSRVVMPDYTDKNALLDVSVTAENAKGVRMLLDNGD
                     EVELTPGQPGEFDGGIAAFTGLDNGKHTAIFTPWRDVLVGESKGADYVIALPPPGYEV
                     SWQAGAQDIDGRVVAIEVLPDGRPVELGTYFEAGEPRCYLRLRDETGEPIEFVPVLPP
                     AHCTAVDLTIDRDTGVMHVLVERKSGDGLKWWVGEFAGWGTGPKNIGIGEVGDTALAL
                     ASRPGLVAVCGAKPVATIDERDALAVLLRPGQPAQERLFDYRPKPNPHLFGEIVRDCT
                     FAGDTLVLVGEANGKHDPEFPNKHDRLVVIEYDVAAESEPVWTVAGPGPGVQSRGLAL
                     DIDDEGRYHLAGYTCLETCAPEGEYRVYSPGGKLEWQASLGPLGSDWFGPHDIAWSPA
                     GYVVVALGAMQGKSSVFKVQALTRGLYMPLWTFIPNDKQVPQMALAVAIGLHGEVYAG
                     GIGAMNHPAFAVIGS"
     gene            complement(6769..7425)
                     /locus_tag="VGB85_16100"
     CDS             complement(6769..7425)
                     /locus_tag="VGB85_16100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0135608.1"
                     /translation="MQPRLLLADDHTLVLEMLVSFLCRDFTVVGTVQDGGSLLEAALR
                     LQPDLIVLEHALPGTDGLTVVRTLRTQLPTARCVFVTRHADLHHVRDAFAAGARGYVL
                     KDATPQELVAGLWRVSRGETHVSPSLGLEPNWAPPTAVTREGSGELTARQIEVLRLVA
                     EGCTGKQIASELNISLKTVESHKACISRQLGLRSTAALTRYAVEHGLLRERGVSTQRA
                     "
     gene            complement(7508..8221)
                     /locus_tag="VGB85_16105"
     CDS             complement(7508..8221)
                     /locus_tag="VGB85_16105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0135609.1"
                     /translation="MTEDPPTTETTPGETPVPPTLAVPRPRVLIAEDHTLVREGLRIL
                     LSGEVEVVDTVEDGRALLSAAERLRPDVILLDISMPLLNGLDAARQLAQHKSTAKLVF
                     VTMHADPAYVRQAFRAGASGYVVKSSASAHLTEAIQEVVAGRRYISPIPGTDHADLLT
                     GDPATDGDELSDNLTPRQREILQLVAEGRTAREIAGVLGISRKTVEFHKASIMRLLRL
                     RTTAELTRYAMAHGIIASA"
     gene            complement(8218..8499)
                     /locus_tag="VGB85_16110"
     CDS             complement(8218..8499)
                     /locus_tag="VGB85_16110"
                     /inference="COORDINATES: protein motif:HMM:NF022829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HEY0135610.1"
                     /translation="MSMRREQLDAWTRPGNHLSRHLAHLRDAAWPATREELIDFVART
                     SGPLQLLDDLYALPEDGTRYATPEIVLAPAQSLHFHWPPWHPQQDHGAA"
     gene            complement(8626..9870)
                     /locus_tag="VGB85_16115"
     CDS             complement(8626..9870)
                     /locus_tag="VGB85_16115"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0135611.1"
                     /translation="MPDAPAPDRHARSKALLRRAREIIPGGYHLTGRPIGGPDEAPMY
                     IERGKGARCWDVDGHEYIDYFMAFGPNLLGHAHAEVDDAAIEQVRRGALLSLNHPLHV
                     RFIERLIARFPGAEMGAFFKSGSDATTAALRLARRATGRRKVARCGYHGWHDWCLPQE
                     PFVPAGLDEQVPEFSAKDPESLRALLGARPGEFAAVIVAPEMVYPPRPEVFVALAEIT
                     RKHGAVFVMDEVKTAFRVAPGSMQQRLGVVPDLTTLSKALGNGWPVAAVIGKREVMRH
                     GAELHMSATYHGETAGIAAALAVLDILDREDVTGHVWRLGERLINGLNDSARRHELPA
                     LAYGEPVPPMPFMRFTHDDPAVNDRLRAGFFAEVIAAGILLHPRHLWFISHAHTAADI
                     EHTLAVADRAMAGLQGRVAGSI"
     gene            complement(9901..>10141)
                     /locus_tag="VGB85_16120"
     CDS             complement(9901..>10141)
                     /locus_tag="VGB85_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135612.1"
                     /translation="LGHAAVAACLERQQVELLVLPWPMQDPILRDTLPRQALLAGAKI
                     ELVSGAAAERLNTEGGVGARLFYVIKSEIPDETQA"
BASE COUNT         1576 a         3671 c         3436 g         1458 t
ORIGIN      
        1 cgcgacccgc cgttctccaa gctcgacctg atcctctgcc gcaacgtgct catttacttg
       61 ggcaccgtcc tccagcgcaa gctgatgaac gtgttccact acgcgctcaa gcccagcggc
      121 tacctgatgc tcggcgccgc cgagacggtc ggcgggcagg gcgagctgtt cgcggtcgtc
      181 gacaagaagc accgcatctt cgccaagaag ctggtcgaga ggtcgatcta cgccgacctc
      241 ccgatcgagt accgggccgg gcgcggcccg acccccggcc cgcgggtcac cagcgagccc
      301 gtgccggcct cggacatgca gtccgaggtc aaccgcctga tcctcgaccg ctacggcccg
      361 ccgggggtcg tcgtcgacgc gcagttccag atcatccagt ttcgcggccg gaccggcgac
      421 ttcctcgagc ccgcccccgg cgacgccgcg cagagcctca acgtcctcaa gatggcccgt
      481 gagggcctgc tgcacggcct gcaggccgcg ctgttcaacg cccgcaagac cgaggctccc
      541 gtgcgcaagg agggcctgcg cgtgcactcc ggcggccacg tccgcgagat ccacctgcag
      601 gtcgtgccgc tcgccgcccc gggcgaggcc cgccactacc tcgtgctctt cgaggacgtg
      661 cgcgccgggg acgacgaccg cccgccgccg ccgccgccgc tcgcggtcga cgaccacctg
      721 tcgcagatcc aggacgagct cgccgccagc cgcgagtacc tgcaatccat catccaggac
      781 ctcgaggcgg ccaacgagga gctgcaatcc gccaacgagg agatcctctc gagcaacgag
      841 gagctgcaga gcaccaacga ggagctcgac accgccaagg aggagctgca aagcaccaac
      901 gaggagctca acaccgtcaa cgaggagctg cacggccgca acgaggagct cagccgcgtc
      961 aacagcgacc tgatcaacct cctcggctcc gtcgagatag cgatcgtcat cgtcggcgcc
     1021 gacctggcga tccgccgctt cacgccgatg gcggagaaga tcctcaacct gatcccgacc
     1081 gacctcgggc gcccgatcag ccagatcaag cccaacatcg actgccccga gctcgagcgc
     1141 atgatcaccc acgtgatcgc cacgatcgag atccaggagc agaacgtccg cgaccgcgac
     1201 ggcaactggt acacgctgcg catccggccc tacaagaacg tcgacaaccg gatcgacggc
     1261 gccgtgctgg ccctcttcaa catcgccggt cgccgccacg agctcgagct gcgccaggcg
     1321 cacgacaaca tcgccgcgat catcgagacc gcccacgagc cgatgatcgt cctcgacggc
     1381 catctacgcg tgcatttcct caatcacgcg ttctcccgcc gcttcgccgt cgcgcgcgag
     1441 cgcgccctcg agcgccagat cttcgacctc gccgacggcg cctggaacgt cccccagctg
     1501 cgccacctgc tcgccgggct cacccccggc cgcaaccgcg tcgaggccgt ccgcctcacc
     1561 cacgacttcg gcgcggccac cccccggcac gtgcgcatca acgccagctg catccccggc
     1621 ttcgagccga gcgcgagctt cgtggtcctc gcgatcaccg acgccgagcc cgacgccggg
     1681 atgggggcgg gcacctgacc ccgtcggtcg gcgcatgacc gacgccgacg aaaaggcccg
     1741 gctcctcgcc gagaacgccc agctgcgcca cacgctggcg cggcaggagg ccgagcactt
     1801 cgccgagcgc acgaccctgc ggatcgagct cgagacgctc gaccaccagc gccactacct
     1861 cagcgagtcg gtcgactggt acgccgagct ctacgagttc gcgccgatcg cctacctcgc
     1921 gctcgacgcc accggcctga tccgcgacat caacctcacc ggcaccgcgc tgctcggcac
     1981 cgagcgccag aacctgctcg gtcggccgat gcgcctgcac gtcgtctccg aggaccgccg
     2041 gatcttcctc gagcacatct atcgcagccg tcgcaccaac ggagcgatcg tcagcgagct
     2101 gcggatcctc gccgcggagg gccgcgagct cccggcccag ctgatcagcc gccgcggctt
     2161 cggcggcgac ggcccggtca ccctgcgcac cgcgatcttc gacctcagcg agcgcaagcg
     2221 ggccgaggcc gatctgctgc tcggtcaccg ccgcctgagc ctcgcgctcg ccgtcagcca
     2281 ggccggcctg cacgagtacg tcgtcggccg gcccggcatg cagtccagcg cgcgctggtt
     2341 ggagatcctc ggcctgccgc ccgtcgacgc cgacgacgcc gccctgcgcg cgtggttcga
     2401 ggggcgcatc gagccggccg acgccgaggc ccgcgcgcag gccctcgcgg ccttcctcgc
     2461 cggcaccacc ccgacccacg tcgccgaatt tcgcgtgcag cgccacgacg acgcctggat
     2521 ctgggtgcgc gagctcgccg acgcggccca gcgcaccccg gacggccgcc cgagccgcgt
     2581 cgtcggcgtg ctgctcgaca tcagcgatga gaagcgccgg ctcgccgagg ccgagcagcg
     2641 caccgcccag ctgcgcgagc tctcggccgc gctctaccag gtcgaggaga acgagcgccg
     2701 cgagctggcg acgctgctgc acgacgacct cggccagcag ctggtcgccg cccagctcca
     2761 gctgtccgcg ctcgggcgca agctggacgg ccccccaccg gccagcctcg gcctggtggt
     2821 gaagatgcta cgcgaggcgc aggacacgat ccgctcgctg agcttccagc tcagcccgcc
     2881 gatcctgcac gagctcggcc tgatcgccgc gctgcgctgg ctggctggcg agctcgagcg
     2941 caccctcgag ctcgccgtct cgatcgaggc cgacgagccg ctgccgccgc tgcgcgggga
     3001 ccccgaggtc gtgctgttcc gctgcgtccg cgagctgctg ctcaacgtcg ccaagcacgc
     3061 cggcgtgagc gaggtgcgcg tcgtcgtccg cgacgtcccg gactccccgt attgccagat
     3121 cctcgtcgag gatcacggcg tcggcttcgc ggccgaccgc ctgacccgcg agcgctccaa
     3181 ggcccgcagc ttcggcctgc tcagcgtcta cgagcgcatc cagggcctcg gcggccgcgt
     3241 gctcgtcacc agcgcgccag ggcagggcac ccgggccctg ctgatgctcc cacgcgacga
     3301 tgacctcgac gacgatgaag aagtcagcga ggtcgacggg gtagacgagg tagacggggt
     3361 agacggggta gacggggtag acgaggtaga cggggtgctc gaccccctcg aagactaagg
     3421 cgcgctgacc aggctcgctg cgcggacgca gcaggccaat gatcgccgcg ttcgcgccgc
     3481 gacgccggat cgccacgctc gccgagcccg gctggtggcg ccggcccctg acccccggga
     3541 atcccgggcc cacgttcgcg cgcctcgtgc gcgccgcgtc gaagtctact gaatgttaaa
     3601 gtcgatgtaa ttgctcgtac atattttctc ggtctgcgac ctcgtgcgac cgctcggcgt
     3661 gccttatttt tttttaaaaa gggcgcgtcg gcccgcccga ttgtcaggca cacgggcgta
     3721 gatctccggg cgaaaatgcg ctatgttgca gcgatgctca ctcggcacac ctggacgctc
     3781 ctgctggtca cggcttgcca ggggtcgacc gcgaatcaga ccggcttcgg ctcggggccg
     3841 gagcccacga cgacgcccct cgcgtcgacc ggcgagggtt cgacgagctc gagcagcacg
     3901 gggacgtcga cgagcgaggg cagcggctcg gggagcagcg cggccgacac gtcggccgag
     3961 ccgccgcgtg acctcggggt gatgccggat tttggtaacg gcgcgccgat cggctgccag
     4021 ggcaagatcg atctcctgtt cgtgatctcg cgccacggga acatgctgta ccgccaggag
     4081 cagctcgccc tggcgttccc tcagttcgtc gccgcgatcg aggcgaagtt caccaacttc
     4141 gactatcaca tcatggtcgt gaccggcgac cccgggtggg gtaacgacac ctgcacgcag
     4201 gtctgcccga cccccacgtg caagatcggc gaagattgct gcccgttgtc cccgcaaaac
     4261 ggagatacgg ggcagccttg ctgccacatg cctgactacc cgtgtcagga cctcgacctc
     4321 gtgaccccgt gcgaccgcac ctggggggcc ggcgaggtgt tccccgccgg taccgccacg
     4381 cagaacaagc cgtgcgcgat cgacggcgac cgccgctacc tcgtgaaggg gcagaccgac
     4441 ctcgccgaga ccttcgagtg catcgccacg gtcggggcca gcggctatga cttgctcgga
     4501 caggcgctca cggccgccat gcaaaagccc atcaacgatc cgggcggctg caacaacggc
     4561 tttcttcgcg acgacgcgct gttgatggtg acgttcatct ccaccaccgc ggacacgccg
     4621 acggactccg agggcacacc ggccgagtgg gcaaaggccg tgctcgacgc gaagcacggc
     4681 gatgacaggt ccgtcgtcgc gttcgacatc ggcggcggtg gccccgagtg cctgtactac
     4741 gatcggatct gccacatgac cgaggcgttt ccgtaccatc accacgtcta cgtcgaggaa
     4801 gccgactacg ggccggggtt cctcgcggcg gcgagcctcg tcgagaccgc gtgcgccggg
     4861 ttcgtggtcc cgggttgagg ccgacgtcta gctgccgatg accgcgaacg cggggtggtt
     4921 catggcgccg atgcctccgg catagacctc gccgtggagg ccgatcgcca ccgcgagcgc
     4981 catctggggg acctgcttgt cgttcgggat gaaggtccac agcggcatgt agaggcctcg
     5041 cgtgagggcc tgcaccttga acacggagga ctttccctgc atcgcgccga gagcgacgac
     5101 gacgtagccg gccgggctcc acgcgatgtc gtgcgggccg aaccagtccg acccgagcgg
     5161 gccgagcgac gcctgccact cgagcttgcc gccgggggag tagacgcgat actcgccctc
     5221 cggcgcacag gtctcgaggc aggtgtagcc ggcgaggtgg tagcggccct cgtcgtcgat
     5281 gtcgagcgcg aggccccggc tctggacgcc gggcccgggc ccggccacgg tccacacagg
     5341 ctccgactcg gcggcgacgt cgtactcgat gaccacgagg cggtcgtgct tgttggggaa
     5401 ctcgggatcg tgcttaccat tcgcctcgcc gacgagcacg agcgtgtcgc ccgcgaaggt
     5461 gcagtcgcgg acgatctcgc cgaacaggtg cgggttgggc ttcggtcggt agtcgaacag
     5521 ccgctcctgc gccggctggc cggggcggag cagcaccgcg agcgcgtcgc gctcgtcgat
     5581 cgtggcgacc ggcttcgcgc cgcagaccgc gaccaggccg ggccggctcg ccagggcgag
     5641 cgccgtgtcg ccgacctcgc cgatgccgat gttcttcggt ccggtccccc agcctgcaaa
     5701 ctcgccgacc caccacttga gcccgtcgcc gctcttgcgc tcgaccagca cgtgcatcac
     5761 gccggtgtcg cggtcgatcg tcaggtcgac cgccgtgcag tgtgccgggg gcagcaccgg
     5821 cacgaactcg atcggctcgc cggtctcgtc gcgcaggcgc aggtagcagc gtggctcgcc
     5881 cgcctcgaag taggtgccga gctcgacggg cctgccgtcc ggcagcacct cgatcgcgac
     5941 cactctcccg tcgatgtcct gtgcacccgc ttgccacgag acctcgtagc cgggcggcgg
     6001 cagggcgatc acgtagtcgg cccccttcga ctcgccgacg agcacgtcac gccagggcgt
     6061 gaagatggca gtgtgcttgc cgttgtcgag gccagtgaac gccgcgatcc cgccgtcgaa
     6121 ctcgccaggc tggcccggcg tcagctcgac ctcgtcgccg ttgtcgagca gcatgcgcac
     6181 gcccttggcg ttctcggcgg tgacgctgac gtccaggagc gcgttcttgt cggtgtagtc
     6241 cggcatcacg acgcggctga cgatcagcgg cagctcggcc ggctcgcccg tggtgtcgat
     6301 ggtgtcggtg ccgccctccg cgtcgggcgc ggtggagccg gagctgtgat cgacgctgtc
     6361 acccgtgacg gtgtcggcgc tgtcgccggt cgtcggcgtc tggtcgccgg tggagcccgg
     6421 ctgcgccgtg gaagtgctgc acccgtcttc gcagaccatg ccactcggcg gatcccagtc
     6481 gggaacacac gcgagaaccc cggcgacgat gatggcgagt gcgaagcgaa gggcgaggcg
     6541 aaacggcatg gcgcgattcc gatctacgct caaaatcggc cggtgaacag caagcccgtg
     6601 cgaacaggct gccgcagccc gcgccttgcg acacgcgtgt gcgctgagta tgacgacagc
     6661 agccagcagc cgctgctccg gtggtaaccc cgctcgccac gaggtctccc cgcaggtccc
     6721 tcggaaccgg cgcaacgccg ggagcccctc gaggaccagg cgcgctgact aggcgcgctg
     6781 ggtggacacg ccgcgctcgc gcagcaggcc gtgctcgacc gcgtagcggg tcagggcggc
     6841 cgtcgaccgc agcccgagct ggcgcgagat gcaggccttg tgcgactcga ccgtcttcag
     6901 ggagatgttg agctcactgg cgatctgctt gccggtgcaa ccctccgcga cgagccgcag
     6961 gacctcgatc tggcgcgccg tcagctcccc ggagccctcg cgcgtgaccg ccgtcggcgg
     7021 ggcccagttg ggctcgaggc ccaggctcgg gctgacgtgc gtctcgccgc gactgacccg
     7081 ccacagcccc gcgaccaact cctgcggcgt cgcgtccttg agcacgtagc cgcgagcgcc
     7141 cgccgcgaag gcgtcgcgca cgtggtgcag gtcggcgtgg cgggtgacga acacgcagcg
     7201 cgcggtcggc agctgggtcc gcagcgtgcg cacgaccgtc aggccgtcgg tgcccggcag
     7261 agcatgctcg agcacaatga gatcgggctg caagcgtaag gcagcctcga gcagcgaccc
     7321 gccgtcctgg accgtgccga ccacggtaaa gtcccggcaa aggaagctga ccaacatctc
     7381 gaggacgagg gtgtgatcat cggcgaggag gaggcgtggt tgcatggtgg gctcgtgttc
     7441 gcgccaaaga taaaccacca tggtccgggt tgcgagaccc cgccacaccc cagatcgccg
     7501 ccgcggctca ggcgctggcg atgatgccgt gggccatcgc gtagcgcgtc agctccgccg
     7561 tcgtgcgcag gcggagcagg cgcatgatgc tggccttgtg aaactcgacg gtcttgcgtg
     7621 agatgcccag gaccccggcg atctcgcggg ccgtacgccc ctcggcgacc agctggagga
     7681 tctcgcgctg gcgcggggtc aggttgtcgg acagctcgtc gccgtcggtc gcggggtcgc
     7741 cggtcagcag atcggcgtgg tcggtcccgg ggatcggcga aatataacgc cgtcccgcca
     7801 cgacctcctg gatcgcctcg gtcaggtgcg cggaggccga gctcttgacc acgtaaccgc
     7861 tcgcgcccgc gcggaacgcc tggcgcacgt aggccgggtc ggcgtgcatc gtcacgaaca
     7921 ccagcttggc ggtcgacttg tgctgcgcga gctggcgcgc cgcgtcgagg ccgttgagca
     7981 gcggcatcga gatgtcgagc aagatgacgt cgggccggag gcgctcggcc gcggacagca
     8041 gggcacgccc gtcctcgacc gtatcgacga cctcgacctc accggagagc aggatccgca
     8101 ggccctctcg gacgagcgtg tggtcctccg cgatcaacac gcggggccgt gggacagcga
     8161 gggtcggggg caccggggtc tcgcctggcg tggtctctgt agtcggtggg tcttcggtca
     8221 agcagctccg tgatcttgct gaggatgcca ggggggccag tgaaagtgca gcgattgcgc
     8281 gggcgcgagc acgatctccg gcgtcgcgta gcgcgtgccg tcctcgggca acgcgtacag
     8341 gtcatcgagc agctgcaacg gcccactcgt gcgcgcgacg aagtcgatca attcctcccg
     8401 cgtcgccggc caggccgcgt cgcgcagatg cgcgaggtgg cgggacaggt gattgccggg
     8461 ccgcgtccac gcatcgagtt gttcgcgcct catggacatg ctcacaggca cggtaacccc
     8521 ctggcgcgat tccagatatt ggggaaatac ccctggaagt ggcgaataaa cggcgctcag
     8581 gtccgaccct cagatcggcc ttcagatcgg ccttcagatc ggccttcaga tcgacccggc
     8641 cacgcgcccc tgcaggcccg ccatcgcccg gtcggcgacg gccagcgtgt gctcgatgtc
     8701 cgcggccgta tgcgcgtggc tgatgaacca caggtggcgc gggtgcagca agatgcccgc
     8761 cgcgatcacc tcggcaaaga agcccgcgcg caggcgatcg ttgaccgccg ggtcgtcatg
     8821 cgtgaagcgc atgaacggca tcggcggcac tggttcgccg taggccagcg ccggcagctc
     8881 gtgacggcgc gccgaatcgt tgagcccatt gattaaacgc tcgccgagcc gccacacgtg
     8941 gccggtcacg tcctcgcggt cgaggatgtc gagcaccgcc agcgccgcgg cgatgccggc
     9001 cgtctcgccg tggtaggtcg cggacatgtg cagctcggcg ccgtggcgca tcacctcgcg
     9061 cttgccgatc accgcggcga ccggccagcc gttgccgagc gccttgctca gcgtcgtcag
     9121 gtccgggacg acgccgaggc gctgctgcat cgacccgggg gcgacgcgaa acgccgtctt
     9181 cacctcgtcc atcacgaaca ccgccccgtg ctttcgcgtg atctcggcca gcgcgacgaa
     9241 cacctcgggc cgcggcgggt acaccatctc cggcgcgacg atcacggccg cgaattcacc
     9301 cgggcgcgcg ccgagcaagg cgcgcaggct ctcggggtcc ttggcggaga actcgggcac
     9361 ctgctcgtcg aggcccgccg gcacgaacgg ctcttgcggc aaacaccagt cgtgccaccc
     9421 gtggtagccg cagcgcgcga ccttgcggcg gccggtcgcg cgccgggcca ggcgcagcgc
     9481 cgcggtcgtc gcgtcggatc cggacttgaa gaacgcgccc atctcggcgc cggggaaccg
     9541 cgcgatcagc cgctcgatga agcggacatg caatggatgg ttgagcgaca gcaacgcccc
     9601 gcggcggacc tgctcgatcg ccgcgtcgtc gacctccgcg tgggcatggc cgagcaggtt
     9661 gggcccgaag gccataaagt aatcgatgta ctcgtgacca tcgacgtccc agcagcgggc
     9721 ccccttgccg cgctcgatgt acatcggcgc ctcgtcgggc ccgccgatcg gccgacccgt
     9781 caggtggtag ccgccgggga tgatctcgcg ggcccggcgg agcagggcct tcgaccgcgc
     9841 gtggcggtcg ggtgcaggcg cgtcgggcat gtcgatcttc cgtgatcgcg ggcccggatc
     9901 tcaggcctgg gtctcgtccg ggatctcgga cttgatcacg tagaacaggc gcgcaccgac
     9961 gccgccctcg gtgttgaggc gctccgccgc cgcgcccgag accagctcga tcttcgcgcc
    10021 ggcgagcagc gcctggcgcg gcagcgtgtc gcgcaggatc gggtcctgca tcggccacgg
    10081 cagcaccagc agctcgacct gctgacgctc gaggcaagcg gcgaccgccg cgtgcccgag
    10141 c
//
LOCUS       DASWNJ010000950         2259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_653773, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000950 DASWNJ010000000
VERSION     DASWNJ010000950.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653773"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..308)
                     /locus_tag="VGB85_16125"
     CDS             complement(24..308)
                     /locus_tag="VGB85_16125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135613.1"
                     /translation="MVDALGVSRKTLHKYVQMRLLPAPVLVSDGKSGVRCRWTLIALD
                     HVAFILEQQEIGHTLNEIAAMIAARWGTQDKVPLKTEAPKPAEPGPSGTS"
     gene            complement(440..823)
                     /locus_tag="VGB85_16130"
     CDS             complement(440..823)
                     /locus_tag="VGB85_16130"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY0135614.1"
                     /translation="MAAKRAKSPTRKSRKKKPRIFCYTRDLTEALGVTRRTLYTYVQE
                     SMLPAPILYSDGRTGVLCRWPKNAMEHAHFINEQKAIGYNIAEIKAMVAARWGIQDKV
                     AGRSALDPQGVASNGNSSGEEPPSA"
     gene            974..1327
                     /locus_tag="VGB85_16135"
     CDS             974..1327
                     /locus_tag="VGB85_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135615.1"
                     /translation="METHDTASDSTARPSRETEEDVDLVVIDIRVREPGFYTNRTRTY
                     FVAQDGAVYLVCAPGQPATLQREDDLPPDCDPGVVVDEVLALLGEAADMVGRRGLGQR
                     NTRPLIHLEGTHDPY"
     gene            1314..2096
                     /locus_tag="VGB85_16140"
     CDS             1314..2096
                     /locus_tag="VGB85_16140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135616.1"
                     /translation="MTPIDRCAARHAEIERDHQVVAAALVAGLRRIALDTAADPENVC
                     QRVEDTIAESRAAYRRLNDGRINLRGRRNTPWDDSQDLPAPLVWYRHRGNYHGEFPSM
                     AALGVLLSRAWSVGDDVTDRADAASVAEELHLRGELWTFVHDGVLHVFSKPESPSDAV
                     LARLRAMTKFPATHDSPSKASHSAANEPDPSIDHLTKENVIMITRLTIFHVALVVRVR
                     NPQRLRHRLEVVRGDRRRGKRAVVFQSHQEWLPVARAEGSSS"
BASE COUNT          382 a          746 c          722 g          409 t
ORIGIN      
        1 cctcgggcac cggctggtcc ctatcatgag gtcccgctag ggccgggttc tgcgggcttt
       61 ggtgcctcgg tcttcagcgg caccttgtcc tgtgtgcccc agcgggccgc gatcatggcg
      121 gctatctcgt tgagcgtgtg gccgatttcc tgctgctcaa ggatgaaggc cacgtgatcg
      181 agagcgatca gcgtccagcg acaccggacg ccggacttgc cgtcggagac caggaccggc
      241 gccggcagga gccgcatctg cacgtacttg tgcagggtct tgcgggagac gccgagcgcg
      301 tcgaccaaat cggccgtgta gcagaagggc gaccgggccg gcggcggcct cttccttcgc
      361 ttggtcacct ttttggtcgg tcgcttcgct gccacgggcc gagatggtag ggcagaccgc
      421 cgtccagcgc cagccaggat cacgcggaag ggggttcttc gcctgacgag ttcccattgg
      481 atgccacccc ctgcggatct agcgcggacc tgcctgccac cttgtcctgg atgccccagc
      541 gggcggcgac catggctttg atctcggcga tgttgtagcc gatggccttc tgctcgttga
      601 tgaagtgggc gtgctccatg gcgttcttcg gccagcggca caggaccccc gtgcggccgt
      661 cggagtacag gatcggagcc ggcaacatgc tctcttgcac gtacgtgtag agggtccgcc
      721 gggtcacacc gagcgcttca gtcagatctc gcgtgtagca gaaaatccgc ggcttcttct
      781 tccgcgactt cctcgtcggc gatttcgctc ttttcgctgc cacagcccgg gatcctatcc
      841 cagcgcgcca ggaggcgcca gggaccccag gggagccaat gagcaagctg cacatccaag
      901 gcgcgcgtaa tcgcccgaat aatcgggata ttcgccttga ctaggttaca aaggggttcc
      961 aatactagat gagatggaga cgcacgacac agccagcgac tcgaccgcga ggcccagccg
     1021 ggagacggag gaggacgtcg atcttgtcgt gatcgacatt cgtgtgcgcg aaccgggctt
     1081 ctacacgaac cggacccgga cctacttcgt cgcccaggac ggggcggtgt acctcgtgtg
     1141 cgcgcccggc cagccggcga ccttgcagcg agaggacgat cttccgccgg attgcgaccc
     1201 cggcgtcgtc gtcgacgagg tcctcgcgct tctcggcgag gccgcggaca tggtggggcg
     1261 tcgtggtctg ggccagcgca acacccggcc cctcatccac ctggaaggca cccatgaccc
     1321 ctattgaccg ttgtgcagct cgccacgcgg agatcgagcg cgaccatcag gtggttgcag
     1381 cggccctcgt tgccggtctt cgacgcatcg ctctcgacac cgcggccgac cccgaaaatg
     1441 tgtgtcaacg ggtcgaggac acgatcgcgg agagccgcgc tgcgtaccgc cgtctcaacg
     1501 acgggcggat caacctgaga ggccggcgca acaccccgtg ggacgactcc caggacttgc
     1561 ccgcgccgtt ggtctggtac cgccaccgcg gcaactatca cggcgaattc ccgtcgatgg
     1621 cggccctcgg cgtcctgctg agccgagcgt ggagcgtcgg ggatgacgtg accgaccggg
     1681 ccgacgccgc gagcgtcgcc gaggaattgc accttcgcgg cgagctgtgg accttcgtgc
     1741 atgacggcgt gctgcatgtg ttctcgaaac ccgaaagccc tagcgacgcc gtcctggctc
     1801 gcctgcgggc catgacgaaa ttcccggcaa cacacgactc ccccagcaag gcctcgcaca
     1861 gcgcggccaa tgaacctgac cctagcatcg accacctcac gaaagagaac gtcatcatga
     1921 tcaccaggct caccattttc cacgtcgcgc tcgtcgtgcg ggtgcgaaac cctcaacgcc
     1981 tgcgccaccg tctcgaagtg gtgcggggcg atcgtcggcg aggaaagcgg gcggtcgtgt
     2041 tccaaagcca ccaagagtgg ctgcctgtag ctcgcgccga ggggtcttca tcttgatgtc
     2101 ttgctcgttt gtttcctgaa gcggcacggc caaccagcgg tgccgcagct tcactgctgc
     2161 tgaccacaca ccgcgctcct gtgagcgcat caggaggaat ttgccgtgaa aagaacctcg
     2221 atgtttcggg ccaccgcgtc ggccctgttg ttctcgctt
//
LOCUS       DASWNJ010000951         2463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_122981, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000951 DASWNJ010000000
VERSION     DASWNJ010000951.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122981"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VGB85_16145"
     CDS             <1..241
                     /locus_tag="VGB85_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135617.1"
                     /translation="GTLKFSPFEQTYGDLGLDRPLVIGELVISDSAANRLDALVSNGY
                     AGAWPWSLNADYSLDLPGIHDWAVAHAQLADLPPP"
     gene            459..>2463
                     /locus_tag="VGB85_16150"
     CDS             459..>2463
                     /locus_tag="VGB85_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and VWA domain-containing protein"
                     /protein_id="HEY0135618.1"
                     /translation="MSVAGASCTSHSEGGLPPRPLRASKLATTPAPMSLTASDGTGLR
                     LVSLKARAVVEEPLAFTELHLVFQNPTDRTIEGRFEITMPPHAAISRYAMKIEGAWQE
                     GEVVERRAAQVAFEDALHRKQDPALLENKAGNGFSARVFPIFAHERKELIVSYSQELT
                     SSAEPYRLMLRGLPELAELDASVVLKEPAPAPGVTSLATSAAQTRVIELKRQGWTPDQ
                     DLEVRSSNSSPVVGLRNDTLAVARVSPAGAMPPAPIGGLTVLFDTSASRALDFDRQLV
                     NLSGLLASLREQTGEDFALRVVCFDQTTAEIYAGPASGFGTAQLESISTRRALGASDL
                     RGALAAVATGPVSPRLLIVSDGVATAGATELSQLEAAARVLADAGVQRIDAIVDGGIQ
                     DASTLKAVATAGLPDKGIVADGRLPAATLAHKLASATLPTMQVRVPGAGWVWPEKIEG
                     AQPGDELLVYAHDLPPDAVMKVVLTGTDEIVTEVPGMLVERPLLERAWIGTRIDRLTR
                     VRSTLGPDDRDAREALKQQIVEMSTKNRVLSDFTAMLVLETDWDYARFNIDRTALTDI
                     LTVGATGVTLQNRRTAPPLPATPTALADADAGRHKGEEGRMGKPSDSSRARDYDPEME
                     GRQAGILGQMQADAGHFLASPHGAAFAAGDDDADVWGGLTGTEI"
BASE COUNT          352 a          978 c          824 g          309 t
ORIGIN      
        1 cggcaccctc aagttctcgc ccttcgagca gacctacggc gacctcggcc tcgaccgccc
       61 gctcgtcatc ggcgagctcg tcatctccga cagcgccgcg aaccgcctcg acgcgctggt
      121 cagcaacggc tacgcgggcg cgtggccgtg gagcctcaac gccgactaca gcctcgacct
      181 gcccggcatc cacgactggg ccgtcgccca cgcccagctc gccgacctgc cgccgccctg
      241 acgcccgcgc gcggatcgac ggcgacgggc gatcgtcgcc gtcgacggac ggccgggaac
      301 ctgcgacggt ggtcgctgtc tgaccccgct gtatggcccg caaccgcgtc caagcccgcg
      361 tccctgcctc tgcgcgcgtc ctcgcacatg accgcgagga catgtcctgt agagcatgtc
      421 ccgggcgaca gctgcggtcg gccgccctgc tgctcgcgtt gagcgtcgcc ggggccagct
      481 gcacctcgca cagcgagggc ggcctgccgc cccggccgct gcgcgcgagc aagctggcga
      541 cgacgcccgc gccgatgtcc ttgaccgcga gcgacggcac cggcctgcgg ctcgtctcgc
      601 tcaaggcgcg cgcggtggtc gaggagccgc tggccttcac cgagctgcac ctcgtgttcc
      661 agaacccgac cgaccgaacg atcgagggcc gcttcgagat caccatgccg ccgcacgcgg
      721 cgatctcgcg ctacgcgatg aagatcgaag gcgcgtggca agagggcgag gtcgtcgagc
      781 ggcgcgccgc gcaggtcgcc ttcgaggacg ccctgcaccg caagcaggac cccgcgctgc
      841 tcgagaacaa ggccggcaac ggcttcagcg cgcgcgtctt cccgatcttc gcgcacgagc
      901 gcaaggagct gatcgtctcg tattcgcagg agctgaccag ctccgccgag ccctaccgcc
      961 tgatgctgcg cggcctcccg gagctcgccg agctcgacgc cagcgtcgtc ctcaaggagc
     1021 ccgcgccggc gcccggcgtg acctcgctgg cgacctcggc cgcgcagacg cgggtcatcg
     1081 agctcaagcg tcagggctgg acccccgacc aggacctcga ggtgcgcagc agcaattcgt
     1141 cgccggtcgt cggcctgcgc aacgacaccc tcgcggtcgc ccgcgtctcg ccggccggcg
     1201 cgatgccccc ggcgccgatc ggcggcctga ccgtgctgtt cgacaccagc gcctcgcggg
     1261 ccctcgactt cgaccgccag ctcgtcaacc tctccggcct gctggcgagc ctgcgcgaac
     1321 agaccggcga ggacttcgcg ctgcgcgtcg tctgcttcga ccagaccacc gccgagatct
     1381 acgccggccc ggccagcggc ttcggcaccg cgcagctcga gagcatcagc acccgtcgtg
     1441 cgctcggcgc ctccgacctg cgcggggcgc tggccgccgt cgccaccggg ccggtctcgc
     1501 cgcgcctgct gatcgtctcc gacggggtcg ccaccgccgg cgcgaccgag ctctcgcagc
     1561 tcgaggcagc cgcgcgcgtg ctcgccgacg ccggcgtgca gcgcatcgac gcgatcgtcg
     1621 acggcgggat ccaggacgcc agcacgctca aggccgtcgc caccgccggc ctgccggaca
     1681 agggcatcgt cgccgacggc cgcctgccgg ccgccaccct cgcgcacaag ctcgccagcg
     1741 cgaccctccc gaccatgcag gtccgcgtgc ccggggccgg gtgggtctgg cccgagaaga
     1801 tcgagggcgc ccagcccggc gacgagctgc tggtctacgc ccacgacctg ccgccggacg
     1861 cggtgatgaa ggtcgtgctg accggcaccg acgagatcgt gaccgaggtg cccggcatgc
     1921 tcgtcgagcg tccgctgctc gagcgcgcct ggatcggcac ccgcatcgac cggctgaccc
     1981 gcgtccgctc gacgctcggc cccgacgacc gcgacgcccg cgaggcgctc aagcagcaga
     2041 tcgtcgagat gtcgaccaag aatcgcgtgc tcagcgactt caccgcgatg ctcgtgctag
     2101 agaccgactg ggactacgcc cgcttcaaca tcgaccgcac cgcgctgacc gacatcctga
     2161 ccgtcggggc gaccggcgtc actttgcaga accgccgcac cgcgccgccg ctgcccgcga
     2221 caccgaccgc gctcgccgac gccgacgccg gccggcacaa gggcgaggag ggccgcatgg
     2281 gcaagcccag cgactcgtcg cgggcccgcg actatgaccc cgagatggag ggtcgccagg
     2341 ccggcatcct cggccagatg caggccgacg ccgggcactt cctcgccagc ccgcatggcg
     2401 ccgcgtttgc ggccggtgac gacgacgccg atgtgtgggg cggcctgacc ggcaccgaga
     2461 tcg
//
LOCUS       DASWNJ010000952         1549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_229325, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000952 DASWNJ010000000
VERSION     DASWNJ010000952.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229325"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1549
                     /locus_tag="VGB85_16155"
     CDS             <1..>1549
                     /locus_tag="VGB85_16155"
                     /inference="COORDINATES: protein motif:HMM:NF044699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135619.1"
                     /translation="PDLAERFRQRLRIVQAMVRDFQPMKRRMQDLADDYLRAATAEGG
                     ERSLTLRETEGMVRWLCEENYVLLSVEEYDENGRRISALGTASVATPERDPEKFAAYA
                     RDFARTVRYQRSNDESPVHRAGKPGHFVINRVGREGQPVGTCLISGLFTYKALHTPPE
                     EIPFLRVVLRSILADRTISVDSHRGKSVTNAFNSLPLEYLLTQSEDSIWELSDRVLRA
                     ELEGGSDVHIDVGEDGRYVFAFVSLPRGQFSEELRLQVQEKLMREFGATYADFGVYMD
                     RYDNAIVHYYMTGPTPFGAVDTERVRAEVMGLAKGWNERLREALTELVGSGPQVEELF
                     DIYHEAFTEEHKRRAGDQRLLGDLSCLENLRKGAEFDCDLYVSTTGEHPGSLNLRVFN
                     RAAMNLSDELPLIASFGFRVVDEYVRPVHIAHMKGIELDNYRFDVREERIPAIMSRKD
                     EIRNALRAVFAGTMGRDGLNQLIVSTTLTALEVEILRAYVAYLHQVRCPFTTDLLRTV
                     LVANPSVT"
BASE COUNT          250 a          567 c          503 g          229 t
ORIGIN      
        1 gcccgacctc gccgagcgct tccgccagcg cctgcggatc gtccaggcga tggtccgcga
       61 cttccagccg atgaagcggc gcatgcagga cctcgccgac gactacctgc gggccgccac
      121 cgccgagggc ggcgagcgca gcctgacgct gcgcgagacc gagggcatgg tccgctggct
      181 gtgcgaggag aactacgtgc tgctctcggt cgaggagtac gacgagaacg gccgccggat
      241 cagcgcgctc ggcacggcct cggtcgccac gcccgagcgt gaccccgaga agttcgccgc
      301 ctacgcccgc gacttcgcgc gcaccgtccg gtaccagcgc agcaacgacg agtcgccggt
      361 gcaccgcgcc ggcaagccgg ggcacttcgt catcaaccgc gtcggtcgcg agggccagcc
      421 ggtcggcacg tgcctgatca gcggcctctt cacctacaag gcgctgcaca ccccgccgga
      481 ggagatcccg ttcctgcgcg tggtcctccg ctcgatcctc gcggatcgca cgatctcggt
      541 cgactcgcac cgcggcaagt cggtcaccaa cgccttcaac tcgctgccgc tcgagtacct
      601 gctgacgcag tccgaggaca gcatctggga gctctccgac cgcgtgttgc gggccgagct
      661 cgagggtggc tccgacgtgc acatcgacgt cggcgaggac ggccgctacg tgttcgcgtt
      721 cgtcagcctc ccgcgcggtc agttctccga ggagctgcgc ctgcaggtcc aggagaagct
      781 gatgcgcgag ttcggggcga cctacgccga cttcggcgtc tacatggatc gctacgacaa
      841 cgcgatcgtg cactactaca tgaccggccc gaccccgttc ggcgccgtcg acaccgagcg
      901 cgtgcgcgcc gaggtcatgg gcctcgccaa gggctggaac gagcgcctgc gcgaggcgct
      961 gaccgagctc gtcggcagcg gcccgcaggt ggaggagctg ttcgacatct accacgaggc
     1021 cttcaccgag gagcacaagc ggcgcgccgg cgaccagcgc ctgctcggcg acctgagttg
     1081 cctcgagaac ctgcgcaagg gcgcagagtt cgactgcgac ctgtacgtct cgacgaccgg
     1141 cgagcacccc ggcagcctca acctgcgcgt gttcaaccgc gccgcgatga acctcagcga
     1201 cgagctgccg ctgatcgcca gcttcggctt tcgcgtcgtc gacgaatacg tgcggccggt
     1261 gcacatcgcc cacatgaagg gcatcgagct cgacaactat cgcttcgacg tccgcgagga
     1321 gcggatcccg gcgatcatgt cgcgcaagga cgagatccgc aacgcgctgc gggccgtgtt
     1381 cgccggcacg atgggccgcg acggcctcaa tcagctgatc gtctcgacga ccctgacggc
     1441 gctcgaggtc gagatcctgc gcgcctacgt cgcgtacctg catcaggtcc gctgcccctt
     1501 caccaccgac ctgctgcgca ccgtcctcgt cgccaacccc tcggtcacg
//
LOCUS       DASWNJ010000953         2513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_510350, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000953 DASWNJ010000000
VERSION     DASWNJ010000953.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510350"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VGB85_16160"
     CDS             complement(<1..262)
                     /locus_tag="VGB85_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135620.1"
                     /translation="MLKRHAVKIGLSLLMCLALPARPSEAHLAKWGTWEITHRNLMKL
                     FPDADPNAWRIKRYTFSEAEVKFLEAQLGFELYPEDKQPEFFI"
     gene            complement(351..2093)
                     /locus_tag="VGB85_16165"
     CDS             complement(351..2093)
                     /locus_tag="VGB85_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135621.1"
                     /translation="MLSLVPVIGVWLAQAPVQPSERPAEVPLDTTPATPGAPGTPPAS
                     PPAPASQDPGPPPPASGDFFGAGTTAPPPASVEGPGGAPPMPPVEELPPRPGGPSSPS
                     TGPKIVLGGAKDRTPIAGGGAGFFDPGKLSDASTGGGAIQIRGYLGFNFAVTERTNTA
                     MRNPETNTFDRLKTLPYFGGGAANLYVGAPVYSDVVFVRIAFEFISIPRATAGAADVS
                     PAFNPVILMEAAALEVNPFAWASKAPRWFSEGFKATGGVFVVPFGLEDEEHDAPVRWW
                     ATRPLAMSAGRIYPGTWIDIGATIKWKPTFGHGKRPIEVDIGVLNGDACTQTRSNDQL
                     YRFQPVGQFVETCERRLRGVENIPRADGSVLGISPDNNGNKSFVARVMVRPVPALNLG
                     GSFVWGKHPESSFKIPSYQGKTFVDAVQAVTWRAGAHFDLNLDEVFQSRYPLPHIRGE
                     VVYGVDAAAPQDPKNPVLADRHMLGGYAQVAQPLWRRKKTRLPGLIAQYRFDHADPDL
                     DVPRATAGGPVASDFSDEFRYDEALQAHVVGLRFPVLPRFTLKAEYAFVREDGGRGNR
                     LHNDNFTFQAVADF"
     gene            2156..>2513
                     /locus_tag="VGB85_16170"
     CDS             2156..>2513
                     /locus_tag="VGB85_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135622.1"
                     /translation="MRWLLTLTLTMGAACGSGPDTPAVPASGKEAVTTPYVAIQETLA
                     RDSIDGLSQLSARVVTAAEPVQDRTGIGDVIAGAGRVAVQDIDTARLAFSKMSMGLIT
                     YLKQNPSERADLEVVFC"
BASE COUNT          381 a          828 c          929 g          375 t
ORIGIN      
        1 cgatgaaaaa ctcgggctgc ttgtcctcgg ggtatagctc gaagcccagc tgcgcctcga
       61 ggaacttcac ctcggcctcc gagaaggtgt agcgcttgat ccgccacgcg ttcggatcgg
      121 cgtccgggaa cagcttcatc aagttgcgat gagtgatctc ccaggtgccc cacttcgcca
      181 ggtgggcttc gctcggccgg gccggcagcg cgaggcacat cagcagcgaa agaccgatct
      241 tgaccgcgtg gcgcttgagc atttcttgaa agtcccgcga cagtggtgac gatggccgcg
      301 gaggtcaggg acgtcaaggc gctcgaaagc ttcacgcccg ggccatcgac ctagaaatcc
      361 gcgaccgcct ggaaggtgaa gttgtcgttg tgcaggcggt tgccgcgccc gccgtcctcg
      421 cgcacgaacg cgtactcggc cttgagcgtg aagcgcggga gcaccgggaa gcgcaggccg
      481 accacgtggg cctgcagggc ctcgtcgtag cggaactcgt ccgagaagtc cgaggcgacc
      541 gggccgcccg cggtcgcgcg cggcacgtcg aggtcagggt cggcgtggtc gaagcgatat
      601 tgcgcgataa ggcccggcag ccgggtcttc ttgcgccgcc acagcggctg ggcgacctgg
      661 gcgtagccgc cgagcatgtg gcggtcggcg agcaccgggt tcttcgggtc ctgcggggcg
      721 gccgcgtcga cgccgtagac gacctcgccg cggatgtgcg gcaggggata gcggctctgg
      781 aacacctcgt cgaggttgag gtcgaagtgc gccccggcgc gccaggtgac ggcctggacg
      841 gcgtcgacga aggtcttgcc ctgatagctc ggaattttga agctgctctc cgggtgcttg
      901 ccccacacga acgagccgcc gaggttgagc gcgggcaccg ggcggaccat cacgcgcgcg
      961 acgaaggact tgttgccgtt gttgtcgggc gagatgccga gcaccgaacc atcggcgcgc
     1021 gggatgttct cgacgccacg caggcgccgc tcgcaggtct cgacgaactg gccgaccggc
     1081 tgaaagcggt acagttggtc gttgctgcgc gtctgggtgc acgcgtcgcc gttgagcacg
     1141 ccgatgtcga cctcgatcgg ccgcttgccg tgcccgaagg tcggcttcca cttgatcgtc
     1201 gcgccgatgt cgatccacgt gccggggtag atccggccag cgctcatcgc caggggtcgc
     1261 gtcgcccacc agcgcaccgg cgcgtcgtgc tcctcgtcct cgaggccgaa ggggaccacg
     1321 aacactcccc cggtcgcctt gaagccctcg ctgaaccacc gcggggcctt gctcgcccac
     1381 gcaaaggggt tgacctcgag cgcggcggcc tccatcagga tcaccggatt gaaggccggc
     1441 gagacgtcgg ccgcgcccgc ggtcgcgcga gggatcgaga tgaactcgaa ggcgatccgc
     1501 acgaacacga cgtcggagta gaccggcgcg ccgacgtaca ggttggccgc gccgccgccg
     1561 aagtagggca gggtcttgag ccgatcgaag gtgttggtct ccgggttgcg catcgccgtg
     1621 ttggtgcgct cggtgaccgc gaagttgaag ccgagatacc cgcggatctg gatcgcacca
     1681 ccaccggtcg acgcgtcgct gagcttgccg ggatcgaaga agccggcccc gccgccggcg
     1741 atcggggtgc ggtccttcgc gccgccgagc acgatcttcg ggccggtgga tggggaagat
     1801 gggccgccgg ggcgcggggg aagctcctcg accggcggca tcggcggggc tcccccgggg
     1861 ccctcgacgc tcgcgggcgg tggcgcggtg gtgccagcgc cgaagaagtc gccgctcgcg
     1921 ggtggcggcg gcccgggatc ctgactcgcg ggcgcgggcg gagacgccgg gggcgttccg
     1981 ggggcgcccg gggtcgctgg agtggtgtcg agcgggacct cggcaggtcg ctcgctcggc
     2041 tggaccggcg cttgtgcgag ccagacgccg atgacgggga cgagcgagag catgaacgcg
     2101 gagcccggcg gactctacct caggtccggc ggcttgtgat acgccagagg gcgtgatgcg
     2161 ctggctcttg accctcaccc tgacgatggg cgccgcctgc gggtcgggcc cggacacccc
     2221 cgccgtgccc gcctccggga aggaggccgt gacgaccccc tacgtggcga tccaggagac
     2281 gctcgcgcgg gacagcatcg acgggctgtc gcagctgtcg gcgcgggtcg tcaccgcggc
     2341 cgagcccgtg caggacagga ccggcatcgg cgacgtcatc gcgggcgctg gccgggtcgc
     2401 cgtacaggac atcgacaccg cccgcctggc cttctcaaag atgagcatgg ggctgatcac
     2461 ctacctcaag cagaacccga gcgagcgcgc ggacctcgag gtcgtcttct gcc
//
LOCUS       DASWNJ010000954         1629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_329658, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000954 DASWNJ010000000
VERSION     DASWNJ010000954.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329658"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1105)
                     /gene="mnmA"
                     /locus_tag="VGB85_16175"
     CDS             complement(35..1105)
                     /gene="mnmA"
                     /locus_tag="VGB85_16175"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEY0135623.1"
                     /translation="MRIVAAMSGGVDSSVAAALLVEAGHDVVGLTMRLYDAGAAERRG
                     RGGTCCSPAEIDQARDACARLGIPHYTVDERARFEDAVIDDFVRSYVGGRTPNPCVRC
                     NEHVKFGPLLARARALGAEALATGHYAKVEDGELRRADDAGKDQSYFLFAMGLPTLAS
                     LRFPLGTWHKDQVREKARALGLANADAPDSQEICFVGGGDHAAVVEARAAALGLDMDR
                     LAAGPIVDEGGTVVGEHAGVHRVTVGQRRGLHVAGSAPRYVLRVLPETRTVVVGDAEA
                     LKVASLELEDLRRLTPIGEHASVRAQVQLRHRGRPAPATVEVAGTRAVVRFDEPVGAA
                     APGQAAVLYDGARVLGGGWIAG"
     gene            complement(1102..>1629)
                     /locus_tag="VGB85_16180"
     CDS             complement(1102..>1629)
                     /locus_tag="VGB85_16180"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0135624.1"
                     /translation="LALAPLMHGGPQERGRRPGSEAVALIAGFGVAAEVVTRELPDRR
                     AHATALTARLRAGLMQLPSVTIHGDPESHDGVTVNVGFAGCDGQLLLINLDLAGFAVA
                     TGAACSSGSLEPSPVLLALGMPATQARSALRISVGTRTTAQDIDALLEVLPELLGRVR
                     GAAVDDADWREVSVG"
BASE COUNT          210 a          660 c          557 g          202 t
ORIGIN      
        1 ttgctccgct cgctgtcccg cttcgccatg atcgtcaccc ggcgatccat ccgccgccga
       61 gcacgcgggc cccgtcataa aggacggccg cctgcccggg cgccgcggcg ccgaccggct
      121 cgtcgaagcg cacgacggcc cgcgtccccg cgacctcgac cgtcgcgggc gccggtcggc
      181 cgcggtggcg cagctgcacc tgcgcgcgca cgctcgcgtg ctctccgatc ggcgtcaggc
      241 ggcgcaggtc ctcgagctcg agcgaggcca ccttgagcgc ctcggcgtcg ccgaccacca
      301 ccgtgcgcgt ctccggcaag acacggagta catagcgcgg cgcgctgccg gcgacgtgca
      361 accctcttcg ttgcccgacc gtcacgcgat gcaccccggc gtgctcgccg accaccgtcc
      421 caccttcgtc gacgatcggc ccggccgcca gccgatccat gtcgagcccg agcgcggcgg
      481 cccgcgcctc gacgaccgcc gcgtggtcgc ctccgccgac gaagcagatc tcctggctgt
      541 cgggcgcgtc cgcgttggcg aggccgagcg cgcgagcctt ctcccttacc tggtccttgt
      601 gccaggtgcc gagcgggaag cgcagcgacg cgagcgtcgg caggcccata gcgaacagga
      661 agtagctctg atccttgccg gcgtcgtcgg cccgccgcag ctcgccgtcc tcgaccttcg
      721 cgtagtggcc ggtcgccagc gcctcggccc ccagcgcgcg cgcgcgggcc agcagcggcc
      781 cgaacttgac gtgctcgttg cagcgaacgc acgggttcgg cgtccggccg ccgacgtagc
      841 tgcgcacgaa gtcgtcgatc accgcgtcct cgaagcgcgc ccgctcgtcg accgtgtagt
      901 gcgggatccc gaggcgcgcg cacgcgtccc gggcctgatc gatctccgcg ggcgagcagc
      961 aggtcccgcc gcgcccgcgt cgctcggccg cgccggcgtc gtagaggcgc atcgtcaggc
     1021 cgacgacatc gtgcccggcc tcgacgagca gcgccgccgc gaccgacgag tcgacgcccc
     1081 ccgacatcgc cgccacgatc ctcacccgac gctcacttcc cgccagtcgg cgtcatcgac
     1141 cgcggccccg cgcacgcgcc cgagcagctc cggcaacacc tcgagcaacg cgtcgatgtc
     1201 ctgcgccgtg gtccgcgtgc cgaccgagat ccgcagggcc gatcgcgcct gcgtcgccgg
     1261 catcccgagc gccagcagca ccggcgaggg ctcgagcgag cccgacgagc acgccgcgcc
     1321 ggtcgccacc gcgaagccgg cgaggtcgag gttgatcagc agcagctggc cgtcgcagcc
     1381 ggcgaagccg acgttgacgg tcacgccgtc gtggctctcg gggtcgccgt ggatcgtcac
     1441 cgacggcagc tgcatgagcc cggcgcgcag cctcgcggtc aacgccgtcg cgtgcgcgcg
     1501 gcggtccggg agctcgcgcg tgacgacctc cgccgcgacg ccgaagccgg cgatcaacgc
     1561 gacggcctcg ctgccgggcc gacgcccgcg ctcctgtggc ccaccatgca tcagcggcgc
     1621 gagggccag
//
LOCUS       DASWNJ010000955         4192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_301091, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000955 DASWNJ010000000
VERSION     DASWNJ010000955.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301091"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VGB85_16185"
     CDS             complement(<1..882)
                     /locus_tag="VGB85_16185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3' terminal RNA ribose 2'-O-methyltransferase
                     Hen1"
                     /protein_id="HEY0135625.1"
                     /translation="MLLTIRTTHQPAEDLGYLLHKHPGRSQSFDLAFGRAHVFYPELT
                     PEACTAALLLEIDPVRLVRGGPSAQADGLLSQYVNDRPYVASSFLSVAIAQVLRSALA
                     GISKDRPALVDAAIPLTARLPCVPCRGGELLLRRLFEPLGYTVNATRLPLDAMEPNWG
                     MSPYYDVELVREGPLRELLAHLYVLIPVLDDEKHYYIGDEEVDKLLRVGEGWLSTHPE
                     RETIASRYLKHRMGLTRMALARLSQGESDPELDDVVVVTHDRAEEGLERAMSLNDERL
                     HTIVELLRSAEVSTVLDL"
     gene            1082..1708
                     /locus_tag="VGB85_16190"
     CDS             1082..1708
                     /locus_tag="VGB85_16190"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEY0135626.1"
                     /translation="MRRLLAGNERYKNGTRSVAALASQLQRDALVAGQSPFAIVLSCS
                     DSRAPAEILFDQGVGDLFVIRVAGNIVAPSGIGSAEFAAATFGTRLVVVMGHTSCGAV
                     TATLQVLRGEGTVASDNIRDIVDRIRPTAQTVLEVGKDRSPEQQLTDAIRANVRNAVD
                     HLRHGSRILEDMVNSGQLVIVGAVYDIATGHVDFFDAPAGLGEALQAR"
     gene            1785..2903
                     /locus_tag="VGB85_16195"
     CDS             1785..2903
                     /locus_tag="VGB85_16195"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEY0135627.1"
                     /translation="MRGAILFNGNAETIPDLVQAAAPLLLSSRHEDPEVAASRRVLLV
                     TAAWGDREHDESGVKQALNAIGLPSKFEDGYDRALVNLSLYHEVSRLSRTAPQLAHAW
                     VEERRVADLARSFYLEHNAHLIQLFRRTLAAGKQADASLTVARLAATRGHEGSENRLL
                     RYALACQLRQALETLEANDDHLVGLLDEIEQRAFDAAGLAYDPAWRAAQARLERRILE
                     ANSIIMFGGHLDLLLDALRFFRLRDALGEALRRGTQIVAMSAGAMVLCERVIIYDDHA
                     TTRRDFQLYDRGLALVRDIQLFPHCMERIQTDDSDNLAYLARRFRHHACVGLNQRSFL
                     LFELDPRRARSFGGGDCVFVFDPEGRKISYHKGETIRV"
     gene            2945..3682
                     /locus_tag="VGB85_16200"
     CDS             2945..3682
                     /locus_tag="VGB85_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135628.1"
                     /translation="MLRGIAIGRLLSWCCLTGFVAAFACGNDDGDTNEYCEPGNDGCA
                     CLDGTFCFDDRECIEDKCRFPGIETTSATSEATHATTPTSGDSAEDEGGQTEVGGDSD
                     GFCEASAECGPEQVCSWQYQCGSPLDSVYLLLVKKWVPNDCQGIGDDLVYYKVKVDGV
                     VAATSGETACPGEWGSADTYRPEWALEIELWRTNGSDPDSVVTRFCFLDAADECTQVP
                     AAVLHDGGFTGPAGDSFVDISIAVDYI"
     gene            complement(3690..>4192)
                     /locus_tag="VGB85_16205"
     CDS             complement(3690..>4192)
                     /locus_tag="VGB85_16205"
                     /inference="COORDINATES: protein motif:HMM:NF016206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEY0135629.1"
                     /translation="DGFLSDHYDPQEKALRLSEDVYYGRSLSAVGVACHEAGHALQDA
                     HGYRPLALRSALVPVTQVGSSLSWIVLTAGFVMHMTTMVYLGIALFAASVVFALVTLP
                     VEWDASARAKALMVRAGIVSPSERVHAGSVLNAAFLTYLAAAVTSILTLIYYLVRAGF
                     FARDDE"
BASE COUNT          671 a         1371 c         1534 g          616 t
ORIGIN      
        1 gaggtcgagc acggtcgaga cctcggcgct gcgcagcagc tcgacgatcg tgtgcaggcg
       61 ctcgtcgttg aggctcatcg cgcgctcgag gccctcctcg gcgcggtcgt gcgtgacgac
      121 gaccacatca tcgagctcgg ggtcggactc accctgagac aatcgcgcga gggccatgcg
      181 ggtcaggccc atgcggtgct tgaggtaacg cgaggcgatc gtctcgcgct cggggtgggt
      241 cgacagccag ccctcgccga cgcgcaggag cttgtcgacc tcctcgtcgc cgatatagta
      301 gtgcttctcg tcgtcgagca cggggatcag cacgtagagg tgggccagca gctcgcgcag
      361 cggaccctcg cggaccagct cgacgtcgta ataaggggac atgccccaat tcggctccat
      421 ggcgtcgagc ggcaggcgcg tcgcgttcac ggtgtagccg agcggctcga acagcctgcg
      481 cagcaggagc tcgccgccgc ggcacggcac gcacggcaag cgagcggtca gcgggatcgc
      541 ggcgtcgacg agcgcggggc ggtccttcga gatcccggcg agcgcgctgc gcagcacctg
      601 ggcgatcgcc acgctgagga acgaggaggc gacgtagggc cggtcgttga cgtactgcga
      661 gagcagaccg tcggcctgcg ccgagggccc gccgcggacc agccggaccg ggtcgatctc
      721 cagcagcagc gcggcggtgc aggcctcggg cgtcagctcg gggtagaaca cgtgggcgcg
      781 cccgaacgcg aggtcgaagc tctgcgagcg tcccgggtgc ttgtgcaaca gatagccgag
      841 gtcctcggcc ggctgatgcg tggtgcggat cgtcaggagc acggccggca gtgtatcagc
      901 accgtcggcg gcgtgagtac ggcgggcccg gcaaggtggg cgctgcttcg cccgccgtga
      961 cgtggacgtc gcttgccgga ggaggcgatc catcgcactg tgcgggcgca ctttcgcagc
     1021 gaacccgagc acgaccatgc cgcagacccc cgactctacg cctatcgccc ccatcgacgc
     1081 gatgcgccgg ctgctcgccg gcaacgaacg atacaagaac gggacccgca gcgtcgccgc
     1141 gctggcgagt caactgcagc gggacgcgct ggtggcgggt cagagcccgt ttgcgatcgt
     1201 gctgtcctgc tcggactcgc gggcgccggc cgagatcctg ttcgaccagg gggtcggcga
     1261 cctgttcgtc atccgcgtcg ccggcaacat cgtcgcgccg tccggcatcg gcagcgccga
     1321 gttcgccgcg gcgaccttcg gcacccggct cgtcgtcgtc atggggcaca cgtcatgcgg
     1381 ggcggtgacg gcgacgctcc aggtgctgcg cggggagggc acggtggcca gcgacaacat
     1441 ccgcgacatc gtcgaccgga tccgtccgac ggcgcagacg gtgctcgagg tcggcaagga
     1501 ccgctcgccg gagcaacagc tcaccgatgc gatccgcgcc aacgtccgta acgcggtcga
     1561 ccacctgcgc cacggcagcc gcatcctcga ggacatggtc aacagcgggc aactggtgat
     1621 cgtcggcgcg gtgtacgaca tcgccaccgg tcacgtcgac ttcttcgacg cgccggcggg
     1681 gctcggcgag gccctgcagg ccaggtaacg cggccggggc gcgacgcgcg atctcgcgtc
     1741 gaccgcgtac gccgtaccgg gcgcgcctgg gctaccattg gcggatgcga ggcgccattc
     1801 tcttcaacgg caacgccgag acgatcccgg acctcgtgca ggccgccgcg ccgctgctgc
     1861 tgtcgtcgcg ccacgaggac cccgaggtgg cggcctcgcg gcgggtgctg ctggtgaccg
     1921 cggcgtgggg cgaccgggag cacgacgaga gcggcgtgaa gcaggccctc aacgcgatcg
     1981 ggctgccctc taaattcgag gacggctacg accgcgcgct cgtcaacctc tcgctttatc
     2041 acgaggtcag caggctctcg cgcaccgccc cgcagctggc ccacgcgtgg gtcgaggagc
     2101 gccgcgtcgc cgacctggcg cgctcgtttt acctcgagca caacgcccac ctgatccagc
     2161 tgtttcgccg gacgctggcg gcgggcaagc aggcggacgc gagcctgacg gtcgcgcggc
     2221 tggccgcgac gcgcgggcac gagggcagcg agaaccgcct gctgcgctac gcgctggcct
     2281 gtcagctgcg ccaggcgctg gagaccctcg aggccaacga cgatcacctg gtgggattac
     2341 tcgacgagat cgagcagcgg gccttcgacg ccgccggcct ggcgtacgac ccggcgtggc
     2401 gggcggcgca ggcccgcctg gagcgcagga tcctcgaggc caactcgatc atcatgttcg
     2461 gcgggcacct cgacctgttg ctcgacgcct tgcggttctt tcgcctgcgt gacgcgctcg
     2521 gagaggcgct gcgacgcggg actcagatag tcgcgatgtc ggccggcgcg atggtactgt
     2581 gtgaacgtgt gatcatttat gacgatcacg cgacgacgcg gcgcgacttc cagctgtacg
     2641 accgcggcct cgcgctggtc cgcgacattc agctgttccc gcattgcatg gagcggatcc
     2701 agaccgacga ctccgacaac ctcgcgtacc tggcccgcag gttccgccac cacgcgtgcg
     2761 tcgggctcaa tcagcggtcg tttttgctgt tcgagctcga cccccggcgc gcgcgcagct
     2821 tcggcggggg cgactgcgtg ttcgtgttcg acccggaggg ccgcaagatc agctatcaca
     2881 agggcgagac gatccgggtt tagcaggtgc ttgacaggat cgcgcgtacg ggtaatcgtc
     2941 gatcatgctc cgagggatcg cgatcggccg tctgttgtcg tggtgctgcc tcaccgggtt
     3001 cgtggcggcg ttcgcgtgcg gcaacgacga cggcgacacg aacgaatatt gtgagccggg
     3061 gaacgacggc tgcgcgtgcc tcgacggtac cttttgtttc gatgaccgcg agtgcatcga
     3121 ggacaagtgc aggttcccgg gcatcgagac gaccagcgcg acctccgaag cgacgcacgc
     3181 gacgacgccg acgtccggcg acagcgcgga ggacgagggc gggcagaccg aggtcggcgg
     3241 ggacagcgac ggcttttgcg aggcctccgc ggagtgcggg ccggagcagg tctgtagctg
     3301 gcagtatcaa tgtgggagcc cgctcgattc ggtgtacctg ctgctcgtga agaagtgggt
     3361 gccgaacgac tgccagggga tcggggacga cctggtgtat tacaaggtga aggtcgacgg
     3421 cgtggtcgcg gcgacctcgg gggagaccgc gtgccccggc gagtggggct cggcggatac
     3481 ctaccggccg gagtgggcgc tggagatcga gctgtggcgg accaatggca gcgaccccga
     3541 ctcggtggtg acgcgctttt gcttcctcga cgcggccgac gagtgcacgc aggtgccggc
     3601 cgcggtgctg cacgacggtg gcttcaccgg gccggccggg gacagcttcg tcgacatcag
     3661 catcgcggtc gactacatct gacccgggtc tactcgtcgt cgcgcgcgaa gaagccggcg
     3721 cgcacgaggt agtagatcag ggtcaggatg gaagtgacgg cggccgcgag gtaggtcagg
     3781 aaggcggcgt tgagcacgct gccggcgtgc acgcgctcgg acggcgagac gatgccggcg
     3841 cggaccatca gggccttggc gcgggcgctc gcgtcccact cgaccggcaa ggtcaccagc
     3901 gcgaagacga cggaggcggc gaacagggcg atgccgaggt agaccatcgt ggtcatgtgc
     3961 atcacgaaac cggcggtcag cacgatccac gacaggctgc tgccgacctg ggtgaccggg
     4021 accagcgccg agcgcagcgc gagcgggcgg tagccgtggg cgtcctgcaa ggcgtggccg
     4081 gcctcgtgac aggcgacgcc gacggccgac agcgagcgac cataatagac gtcctccgac
     4141 agtcgcaggg ccttctcctg cgggtcataa tggtcggata aaaagccgtc cg
//
LOCUS       DASWNJ010000956        10566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_301092, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000956 DASWNJ010000000
VERSION     DASWNJ010000956.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 10566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10566
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301092"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VGB85_16210"
     CDS             complement(<1..280)
                     /locus_tag="VGB85_16210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135630.1"
                     /translation="MREPATSPSSLLRCLAGLALLTLPLACRGDTTPPPYAAAVVTRD
                     APPASEPAAAPIAEPGRNLWFPRGPGRDAILARERQDHVAAAALLDQLL"
     gene            complement(427..1365)
                     /locus_tag="VGB85_16215"
     CDS             complement(427..1365)
                     /locus_tag="VGB85_16215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY0135631.1"
                     /translation="MPGITTGGPDTVTSTLTKRPLGDRTGAPPRRAFVVVLAGDRMGE
                     MVELDPNYTTIGRGLTATVRINDEGISRTHAAVTVDKGVYYLSDAGSTNGTFANGERV
                     DKYPLREGDKIQLGAASVLRFTFHEDSDDDLQRDLYESTLRDRLTGLFSRSYLENRLE
                     SDVAMALRHGKPLALGMFAVDNPRAIVEAHGQPVLDQVHRELARRVLEITRSDDLVAR
                     HGDEFVHVCRDVDAMRAARAALRIHGLLAEHSFSTAAGPLRITLCLGVADLALLHEPT
                     ADALLKASESALAMARRAGGNRVEIYDPESEQTRLV"
     gene            1624..1993
                     /gene="ssrA"
                     /locus_tag="VGB85_16220"
     tmRNA           1624..1993
                     /gene="ssrA"
                     /locus_tag="VGB85_16220"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2278..2637)
                     /locus_tag="VGB85_16225"
     CDS             complement(2278..2637)
                     /locus_tag="VGB85_16225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135632.1"
                     /translation="MRTHDVSEQLEIKEFARFEAAQGVRAKPRSRLPDANVYGDDFAL
                     VTVRDGFYADLLRVYYVSRGRVYIVRSPRMRSGQGYRRTSALPEDARPLDESCFDPDD
                     PEDQAVFAVADCIDGIV"
     gene            complement(2992..4236)
                     /locus_tag="VGB85_16230"
     CDS             complement(2992..4236)
                     /locus_tag="VGB85_16230"
                     /inference="COORDINATES: protein motif:HMM:NF014298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY0135633.1"
                     /translation="MVRVVFVAPFNMPATTRFVSAVAELPEVQLALITQEPADVLPPE
                     LRQRLAAHYRVADALDYKHLLPAVRSVGKQLGGIDRILGTLEQLQVQLGQLRDHYQVH
                     GMGEANARNFRDKAQMKDVLRASGVPCARHRRLSSAAEGWVFAREVGFPMIVKPLDAA
                     AAKGTFRVTDSHGLQAALHALQPSADHPCVAEEFVTGLERSFETVSIRGRAVWDSSTR
                     YNPAPLHVLENPWIQWCVLLPREVETSDTQAVRPYARAALEALGMHTGISHMEWFRRD
                     RHDTGPVAISEVGARPPGAQILSINSHAHDTDFYRLWARLVVHETFDPPTRKYAAGAA
                     FFRGQGGDPRGPRRIARLHGLEQAQKELGHLVVEAKLPQVGQPRSDSYDGEGYAIVRH
                     PDTAVVEKALERLVSLVRVELA"
     gene            complement(4278..5009)
                     /locus_tag="VGB85_16235"
     CDS             complement(4278..5009)
                     /locus_tag="VGB85_16235"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY0135634.1"
                     /translation="MEKLRSTWYSTRLRKNVTAVRFGHYGAPLLLFPTAGGDAEECER
                     MLMIKALSPLIDAGRLKVYSCDSVAGQTWIDKNASPGHKAWVQNQYDGYVYEELVPHI
                     RRDCDSSDIEILTAGASLGGFNAVASVCRHPDVFKTAVSMSGTFDFTGWMEGQHPVDF
                     HMASPIHFLRHLEEGHQLRALRRRFIIFAVGEGRWERPSNSWGMAKVLGQRGVPNRVD
                     MWGRNHDHDWPSWREMLPKYMSEMV"
     gene            complement(5027..6703)
                     /locus_tag="VGB85_16240"
     CDS             complement(5027..6703)
                     /locus_tag="VGB85_16240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135635.1"
                     /translation="MANVTRNIGLSLGADICWPGCYEEIVKRLNLSVPHGGDTLDFHV
                     ERVTVEPYYLRQPVRYDLVLDRLTHWFHTSREWIKKAVIMDGLYVLNNPWSLQSMEKH
                     TTYSAMMKLGMPIPETWMIPPKEMMSDNPDVHPTMERYGRHFKLDEIGERLGYPFFMK
                     PYDGGAWVGVSRIDNPRQLHEAYESSGKRVMHLQKAVDPFDLFVRAIGVGPQVHIVKY
                     DPAAPLHDRYKVDFFFVDGEEWQILTDTCLTINAFFGWEFNSCESLRKDGIFHPIDFA
                     NACPDFQVTSLHYHFPELVKNMVRWTLFCAATKREMQMNQDWKPFYAIAKQDLPYNER
                     LKGYAKIANERMETDRFREWCDKHLSHLDEVALEFFGTDVAKGYVRQKVAALFPQHEV
                     PRFTEHFWGLIQFWRKTETDRLNHTKAAKQAQAEQGQQPIHIDTQAPQEAPQFQSPGE
                     QGSNTAAGPDGGSNDLVSSNVGANDSGSDAGTSISSTDSAPASGSGEVLMPQVPTAGA
                     VAQEPASAEAGAALMTGTSGKPAPAASKTAAPASKPGGKFNGKKPGKGKN"
     gene            complement(6749..7888)
                     /locus_tag="VGB85_16245"
     CDS             complement(6749..7888)
                     /locus_tag="VGB85_16245"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY0135636.1"
                     /translation="MKSAIQTLLTQGPVDEASITAFLRSHSSFPIVEGRQATFVYHGA
                     AEAVHLRHWVYGLASSQSFERVPGTNLWYHTLEIPPQSRVEYKLEIVQDGNRQWIRDP
                     LNPNLAHDPFGANSVAHGQGYQRPAWTEHDPDARQGTIKEMVFTETALGGPRRVSVYL
                     PARFRETRRYPLLIVHDGGDYVRYASLQTVLDNLIHRNEVAPLVVALTHPVDRLGEYP
                     NNAAHATFIAEQLLPAMQDKFPIYEKPASRCLMGASFGGVAALATAWRYPGTFGRLLL
                     QSGSFAFTDIGESKRGPNFAKVVEFINAFRDAPGKPSEKVYLSCGTYESLIYENRTMV
                     PVLQGTGMEVRYEEARDGHNWENWRDRLRSGLSWLFPGPLWMVYE"
     gene            complement(7895..8938)
                     /locus_tag="VGB85_16250"
     CDS             complement(7895..8938)
                     /locus_tag="VGB85_16250"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEY0135637.1"
                     /translation="MTVGMLLGPQRPTPIVGDAVAMLEVDRVRGDGRIAAITAGWQER
                     EAEDQDMSAAFGNRTVNLMLHARGEAVFREDQDFFAAHRAKQDRLRQLEDLYRLRLAS
                     ALKGARRVQQATAPRDLIEPELEEALQALRLLDAHHVLKVKQIEEDFERQHRPHERPA
                     LRRHLDEIDAILRSCSALAIAGGHIAVLYNRMRLFDLRSRIARRPELPVIAWSAGAMV
                     VSDRIVLFHDSPPQGRGNAEVLGPGLGLFSGVVVLPHASRRLMLRNHDRLTVFARRFA
                     PAVCVALDQRCWIRFNGPHWTLGPTTYQLATDGHLVNTNGDRPTPPSPAPAPASAATA
                     PSTSSTSSTSTSA"
     gene            9007..10227
                     /locus_tag="VGB85_16255"
     CDS             9007..10227
                     /locus_tag="VGB85_16255"
                     /inference="COORDINATES: protein motif:HMM:NF013254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135638.1"
                     /translation="MHVLFVAPHFPSYQRHFVRAMHSVGARVTGIGEAAPEHMDSELK
                     GWMHGYEQVRSVTDEQAVLDAVRRVQRREYVDRLESTIEAHILPIAAVREATKIPGLS
                     VQAAILCRDKPQMKEHLRRHGVPCAASAGISTVDEAVAFVREHGYPVIIKPRAGAGAS
                     GTSRCDNDGQLAQALKEGHVGRGGEVAIEEFIEGHEGFYDTMSVDGVPVLEFVSHYYP
                     NVLEAMRHRWISPYIVSTNRLDAPGYDDLKALGRRVITTMGLGTTATHMEWFYGSKGL
                     KFSEIGARPPGVGQWDVYAAGNDLDIYREWANAVVHGRIERQPSRRFASGIIALRPDR
                     DGRIIGYEGVEEIQRRFGEYVTKAHLPPAGTPTQGIEAGYMANAWIQMRHPDYDAMRE
                     IFEIIGRTIRVRAG"
     gene            10262..>10566
                     /locus_tag="VGB85_16260"
     CDS             10262..>10566
                     /locus_tag="VGB85_16260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135639.1"
                     /translation="MKVLFISPHYPEEMQDFTRGLAEVGAQVYGVGDTPRGSLPQHTQ
                     RHLRDYLQVPGLFDEDEALRRIVAAARSLKPDRVECLWEPCIVLAARVREALGIPGMR
                     "
BASE COUNT         1605 a         3376 c         3930 g         1655 t
ORIGIN      
        1 cgagcagctg gtcgagcagc gcggcggcgg cgacgtggtc ctggcgctcg cgcgcgagga
       61 tcgcgtcgcg tccggggccg cgggggaacc acaggttgcg cccgggctcg gcgatcggcg
      121 cggcggcggg ctcgctggcg ggcggggcgt ccctggtgac gacggcggcc gcgtacggcg
      181 gcggggtggt gtcgccgcgg caggccagcg ggagggtcag cagcgcgagg ccggcgaggc
      241 agcgcagcag cgaggacggg gaggtggcgg gctcacgcat ggagatctcc tcgccggcca
      301 cgcgggaacc agcacggaac gtcgaagcta gcccaccggc ggccaaaccg cgaactatcg
      361 ccgggatgca ccagtggggc cggtggggat cggacgcgag ccgggcgctg ccggctcgcg
      421 ggttttttaa acgaggcggg tctgttcgct ctcggggtcg tagatctcga cccggttgcc
      481 gccggcgcgg cgggccatgg cgagcgcgga ctcggaggcc ttgagcagcg cgtcggcggt
      541 cggctcgtgg agcagcgcga ggtcggcgac gccgaggcac agggtgatgc gcagcgggcc
      601 ggcggcggtc gaaaaactgt gctcggcgag caggccgtgg atccgcaggg cagcgcgcgc
      661 ggcgcgcata gcgtcgacat cacggcagac gtggacgaat tcatcgccgt ggcgggcgac
      721 gaggtcatcg ctgcgcgtga tctccaacac ccggcgggcg agctcgcggt ggacctggtc
      781 gagcacgggt tggccgtggg cctcgacgat cgcgcggggg ttgtcgacgg cgaacatgcc
      841 gagggcgagt ggtttgccgt ggcgcagcgc catggcgacg tccgactcga ggcgattttc
      901 gaggtagctg cgactgaaca ggccggtgag tcggtcgcgc agggtcgact cgtagaggtc
      961 gcgctggagg tcgtcgtcgc tgtcctcgtg gaaggtgaag cgcagcacgc tcgcggcgcc
     1021 gagctggatc ttgtcgccct cgcgtagcgg gtacttgtcg acgcgttcgc cgttggcgaa
     1081 ggtcccgttg gtggagccgg catcggagag gtagtagacg cccttgtcga ccgtaacggc
     1141 cgcgtgggtg cgagagattc cctcgtcgtt gatgcgcacc gtcgcggtca ggccgcggcc
     1201 gatggtcgtg tagttcgggt cgagctcgac catctcgccc atgcggtcgc ccgcgaggac
     1261 gacgacgaag gctcgccgcg gcggcgcgcc ggtgcggtcg ccgagcgggc gcttggtgag
     1321 cgtcgaggta acggtatcgg ggccgccggt cgtgatgcct ggcatgtaac cccgggttct
     1381 cgtggtgcgc aacatcgcga acgccgcccg gccggtccgg gcgctcgcgg gtgcgcctgg
     1441 tcaggcctct gccagcccgc ccgggcgctc ctgcgcgggg tttgccggac acctgcccct
     1501 tgcgcgtgcc ggcgggctgc atcataatgg gggctcgtct cgggggcact ccgtcgctcc
     1561 cggaacggcg gaccagggtt ttaccctggt ctcttgtctg aactggctga ggaagccact
     1621 caagggggtg tcacggtttc gacgggggtt ctgaacgtga gggtcgcgtg ccggggctgc
     1681 ctacatagcc acgtcaaaat atgtaggaaa aacagatgcc aacgatggca actacggccg
     1741 tatggccatc gctgcgtaag taattacgtt tagcgacgtc gctgcccgag gggttccagt
     1801 agggacagcg gtgtaattac aggagctggt gggcgcgcaa gcctcgggcg cgcctgcgag
     1861 aaccagagga tcggacgtgg gggggcctgt cgatgggcaa gctcgcgtct taaacccaaa
     1921 tgcatcgact acgcacgtag tggtcccacc tgatgacttt cggacccggg ttcaactccc
     1981 ggcgcctcca taaaaaaaca gaagcccaga gcaaggtctc gcggcttcga ccgcctcgtc
     2041 ggcagggtcc gtgccctgga cgaggcggct cttttttaag gccacgaaag tggcagcgtc
     2101 gcgagcctcg gctcgtcggc gcggccgacc gcggggcgaa gctcggccac gcggcagccc
     2161 ggccgggcgt gggctttcgc ctcggccaag cggagcctcg cgcgacggcc ggacgagcac
     2221 gctgcggcgc atggccggcc cgacggcgcc gctgttgttc gccgctgttg gggcgagcta
     2281 gacgatgccg tcgatgcagt cggcgaccgc gaacacggcc tggtcctcgg ggtcgtccgg
     2341 gtcgaagcag gactcgtcga gcggccgggc gtcctcgggc agcgcggagg tccgtcgata
     2401 gccctggccc gagcgcatgc gcggcgagcg gacgatgtac acgcggccgc gcgagacgta
     2461 gtacacgcgc agcaggtcgg cgtagaagcc gtcgcgcacc gtcaccaacg cgaagtcgtc
     2521 gccgtacacg ttggcgtcgg ggagccgtga ccggggcttg gcgcggacac cctgcgccgc
     2581 ctcgaaccgg gcgaactcct tgatctcgag ctgctcgctg acgtcgtgcg tgcgcatcgg
     2641 ccccccgtga agctccatta aagcggtgga agctcccgcc gggtcaaggg cagatctcca
     2701 tggcccggga cacgcctgcg agcgcgcgtg gggccggtcg cgcgcggtcg cgagagaacg
     2761 ccgctccgct ggcagcggcg cggccgattc gctgtttatc gacccacggc gcgtacagcg
     2821 gcgatcttgc gtgggcgcgc aagtaagcat ccgcaggcgc aggccggcgc gtgtgccggc
     2881 gggccggtca ccgcgtgcgg ctcatggcgc gctcggaagt atccggagga cccgaaggac
     2941 atgtccgcga ggacatgtcg ctaaagctcg ccaaaaggcg gcgcggtgga ctcaagcgag
     3001 ctcgacgcgc acgagcgaca ccaggcgctc gagcgccttc tcgacgaccg ccgtgtccgg
     3061 gtggcgaacg atcgcgtagc cctcgccatc ataagaatcg gagcgaggct ggccgacctg
     3121 cggcagcttg gcctcgacga cgaggtgacc gagctccttc tgggcctgct cgaggccgtg
     3181 caggcgggcg atccgtcgcg ggccgcgagg atcgccgccc tgcccgcgga agaacgccgc
     3241 gccggcggcg tacttgcggg tcggcgggtc gaaggtctcg tgcacgacga ggcgcgccca
     3301 caggcgataa aagtcggtgt cgtgcgcgtg cgagttgatt gaaaggatct gcgcgccggg
     3361 cgggcgggcg ccgacctcgg agatcgccac ggggccggtg tcgtggcggt cgcggcggaa
     3421 ccactccatg tggctgatgc cggtgtgcat gcccagcgcc tcgagcgcgg cgcgggcgta
     3481 ggggcgcacg gcctgggtgt cgctggtctc gacctcgcgc ggcaacaaca cgcaccactg
     3541 gatccacggg ttctcgagca cgtgcagcgg cgcggggttg tagcgcgtcg acgagtccca
     3601 cacggcgcgg ccgcggatgc tgacggtctc gaacgagcgc tcgaggccgg tgacgaactc
     3661 ctcggcgacg cagggatggt cggcgctcgg ctgcaaggca tgcagggcgg cctgcaatcc
     3721 gtgtgagtcg gtgacgcgaa acgtgccctt ggccgcggcc gcgtcgagcg gcttgacgat
     3781 catcgggaag ccgacctcgc gcgcgaacac ccagccctcg gcggccgagc tcaggcgccg
     3841 gtggcgtgcg cacgggaccc cggatgcgcg caggacgtcc ttcatctgcg ccttgtcgcg
     3901 gaagttgcgg gcgttggcct cgcccatgcc gtgcacctga taatggtcgc gcagctggcc
     3961 gagctggacc tgcagctgct cgagggtgcc gaggatgcgg tcgatgccgc cgagctgctt
     4021 gccgaccgag cgcaccgcgg gcaacagatg cttgtagtcg agcgcgtcgg cgacgcggta
     4081 gtgagcggcg aggcgctggc gcagctccgg cgggagcacg tcggcgggct cctgggtgat
     4141 gagggcgagc tggacctccg ggagctcggc cacggcgctg acgaagcgcg tcgtggcggg
     4201 catgttgaag ggggcgacga agacgacgcg gaccatggcg gtgctctcca ttcgcgccgc
     4261 gcacgcgcgc gacgacttca gaccatctcg gacatgtact tggggagcat ctcgcgccag
     4321 ctcggccagt cgtgatcgtg gttgcggccc cacatgtcga cgcggttggg cacgccgcgc
     4381 tgaccgagga ccttggccat cccccaggag ttgctcgggc gctcccagcg accctcaccg
     4441 acggcgaaga tgatgaaccg ccggcgcagc gcgcggagct ggtggccctc ctccaggtgc
     4501 cgcaagaagt ggatcggtga ggccatgtgg aagtcgaccg ggtgttggcc ctccatccag
     4561 cccgtgaagt cgaaggtgcc gctcatcgac accgccgtct tgaagacgtc ggggtggcga
     4621 cacacggagg cgaccgcgtt gaagccgccg agcgaggcgc ccgcggtgag gatctcgatg
     4681 tcggaggagt cgcagtcgcg gcggatgtgc gggaccagct cctcgtagac gtaaccgtcg
     4741 tactggttct gaacccacgc cttgtggcca gggctcgcgt tcttgtcgat ccaggtctga
     4801 cccgcgaccg agtcgcagga gtagaccttg agccgccccg cgtcgatcaa cgggctcagc
     4861 gccttgatca tcaacatccg ctcgcactcc tcggcgtcgc cgcccgcggt cgggaacagc
     4921 agcagcggcg cgccgtagtg gccgaagcga acggccgtga cgttcttgcg gagccgggtc
     4981 gagtaccagg tcgagcgcag cttctccatg ccaggccccc gcgcagctag ttcttgccct
     5041 tgccgggctt cttgccgttg aacttgccgc cgggcttgct cgcaggcgcc gcggtcttgc
     5101 tcgccgctgg cgcaggcttg ccgctggtgc cggtcatcag ggccgcgccg gcctccgcgc
     5161 tggcgggctc ctgggcgacg gcgcctgccg tcggcacctg cggcatcaag acctcgccgc
     5221 tgccggacgc gggggccgag tcggtcgagg agatcgacgt gcccgcgtcc gagccagagt
     5281 cgttcgcacc gacattgctc gaaacgagat cgttcgagcc accatccggg ccggcggcag
     5341 tgttcgagcc ctgctcgccc ggagactgga actgcggggc ctcttgcggc gcctgggtgt
     5401 cgatgtggat cggctgctgg ccctgctcgg cctgggcttg cttggcggcc ttggtgtggt
     5461 tgaggcgatc ggtctcggtc ttgcgccaga actggatcag gccccagaag tgctcggtga
     5521 agcgcggcac ctcgtgctgc gggaacaggg cggcgacctt ctggcggacg tagcccttgg
     5581 cgacgtcggt gccgaagaac tcgagcgcga cctcgtcgag gtgcgacagg tgcttgtcac
     5641 accactcgcg gaagcggtcg gtctccatgc gctcgttggc gatcttggcg tagcccttga
     5701 ggcgctcgtt gtacgggagg tcctgcttgg cgatcgcgta gaacggcttc cagtcctggt
     5761 tcatctgcat ctcgcgcttg gtcgcggcgc agaacagggt ccagcggacc atgttcttga
     5821 cgagctccgg gaagtggtag tgcagcgagg tgacctggaa gtccgggcag gcgttggcga
     5881 agtcgatcgg gtggaagatg ccgtccttgc gcagcgactc gcaggagttg aactcccagc
     5941 cgaagaaggc gttgatcgtc aggcaggtgt cggtgaggat ctgccactcc tcgccgtcga
     6001 cgaagaagaa gtcgaccttg taacgatcgt gcagcggggc ggccgggtcg tacttgacga
     6061 tgtggacctg cggtccgacg ccgatcgcgc ggacgaacag gtcgaacggg tcgacggcct
     6121 tttgcaggtg catgacccgc ttgccggagg actcgtaggc ctcgtgcagc tggcgggggt
     6181 tgtcgatacg cgacacgccg acccaggcgc ccccgtcgta gggcttcatg aagaacgggt
     6241 agccgaggcg ctcgccgatc tcgtcgagct tgaagtgccg gccgtagcgc tccatcgtcg
     6301 ggtgcacgtc cgggttgtcg gacatcatct ccttcggcgg gatcatccac gtctcgggga
     6361 tcggcatccc gagcttcatc atcgcggagt aggtcgtgtg cttctccatc gactggagcg
     6421 accagggatt gttgagcacg tacaggccgt ccatgatgac ggccttcttg atccactcgc
     6481 ggctggtgtg gaaccagtgg gtcaggcgat cgagcacgag gtcgtagcgg accggctggc
     6541 gtagatagta cggctcgacg gtgacccgct cgacgtggaa atcgagggtg tcgccgccgt
     6601 gcgggacgct gaggttgagc cgcttgacga tctcctcgta gcagcctggc caacagatat
     6661 cggcgccgag cgacagaccg atgttgcgcg tgacgttcgc catggccgga ctatcggcga
     6721 attgccccag ccttggcaag cgaaagcctc actcgtagac catccacagc ggcccgggaa
     6781 atagccagga caggccgctg cggaggcggt cgcgccagtt ctcccagttg tggccgtcgc
     6841 gggcctcctc gtagcggacc tccatgccgg tgccctgcag gaccggcacc atcgtacgat
     6901 tctcgtagat cagcgactcg taggtgccgc aggacaggta caccttctcc gagggcttgc
     6961 cgggcgcgtc gcggaaggcg ttgatgaact ctaccacttt ggcgaaattc gggccgcgct
     7021 tgctctcgcc gatgtcggta aacgcgaagc tgccggactg caacagcagg cgcccgaagg
     7081 tgccagggta ccgccacgcg gtcgccagcg ccgccacccc gccgaagctc gcgcccatca
     7141 gacaccgcga ggcgggcttc tcgtagatcg ggaacttgtc ctgcatcgcc gggagcagct
     7201 gctcggcgat gaaggtggcg tgcgccgcgt tgttcgggta ctcgccgagg cggtcgaccg
     7261 ggtgggtcag cgcgacgacc agcggggcga cctcgttgcg gtggatcagg ttgtcgagga
     7321 ccgtttgcag gctggcgtag cggacgtaat caccgccgtc gtggacgatg aggaggggat
     7381 agcgccgggt ctcgcggaat cgcgcgggca agtacacgct gacgcgacgc ggcccgccga
     7441 gcgcggtctc cgtgaagacc atctccttga tcgtgccctg gcgggcgtcg gggtcgtgct
     7501 cggtccacgc gggccgctgg taaccctggc cgtgggcgac cgagttggcg ccgaaggggt
     7561 cgtgcgcgag gttggggttg agcgggtcgc ggatccactg gcggttgccg tcctggacga
     7621 tctccagctt gtactcgacg cgcgactgcg gggggatctc gagggtgtgg taccagaggt
     7681 tggtgccggg cacgcgctca aaggactgcg acgaggcgag cccgtagacc cagtggcgca
     7741 ggtgcacggc ctcggcggcg ccgtgataga cgaaggtcgc ctggcggccc tcgacgatcg
     7801 ggaaagagga gtgactgcgc aggaacgcgg tgatcgacgc ctcgtcgacc gggccctgag
     7861 tgaggagcgt ctggatcgct gatttcatgg cgcgtcaggc gctggtcgag gtggacgagg
     7921 tggacgaggt ggaaggcgca gtggctgcgg atgccggtgc tggtgccggc gacggagggg
     7981 tcggccggtc gccgttggtg ttgacgaggt ggccgtcggt ggcgagctgg taggtggtcg
     8041 ggccgagggt ccagtggggg ccgttgaagc ggatccagca gcgctggtcg agcgcgacgc
     8101 acacggcggg cgcgaagcgg cgggcgaaca cggtcaggcg gtcgtggttg cgcagcatca
     8161 ggcgccggct cgcgtgcggg agcacgacga cgccggagaa caggccgagg cccgggccga
     8221 gcacctcggc gttgccgcgg ccctgcggcg ggctgtcgtg gaacaggacg atgcggtccg
     8281 acacgaccat cgcgccggcc gaccacgcga tgaccggcag ctccgggcgg cgggcgatgc
     8341 gagagcgcag gtcgaacagg cgcatgcggt tgtagaggac cgcgatgtga ccgccggcga
     8401 tcgccagggc gctgcagctg cggaggatgg cgtcgatctc gtcgaggtga cggcgcagcg
     8461 cggggcgctc gtgcgggcga tgctggcgct cgaagtcctc ctcgatctgc ttgaccttga
     8521 ggacgtggtg ggcgtcgagc aggcgcaggg cctgcaacgc ctcctcgagc tcgggctcga
     8581 tgagatctcg cggagctgtg gcctgctgga ccctacgtgc acccttgagg gcgctggcca
     8641 gacgcaggcg gtagaggtcc tcgagctggc gcaggcggtc ctgcttcgcc cggtgagcgg
     8701 cgaagaaatc ctggtcctcg cggaacacgg cctcaccgcg ggcgtgcagc atcaagttga
     8761 cggtgcggtt gccgaacgcg gcggacatgt cctggtcctc ggcctcacgt tcctgccaac
     8821 ccgccgtaat cgcggcgatc cggccgtctc cccggacgcg gtccacctcg agcatcgcca
     8881 cggcgtcccc gacgatgggg gtcgggcgct ggggcccgag caacatgccc acggtcatgg
     8941 atctgtccct gggcgcggag ccatggtagg agcgcaatag gctgccccga gggtaggaaa
     9001 cgcatcatgc atgttctgtt cgtcgccccg cacttcccct cttaccagag gcactttgtc
     9061 cgcgccatgc actcggtggg cgcgcgtgtg acgggcatcg gtgaagccgc gcccgagcac
     9121 atggacagcg agctcaaggg ctggatgcat ggctacgagc aggtccgttc ggtcaccgac
     9181 gagcaggcgg tgctcgacgc ggtgcgtcgc gtgcagcggc gcgagtacgt cgaccggctc
     9241 gagtcgacga tcgaggcgca tatcctcccg atcgccgccg tgcgcgaggc gacgaagatc
     9301 cccggcctgt cggtgcaggc ggcgatcttg tgccgcgaca agccgcagat gaaggagcac
     9361 ctgcgccgac acggggtccc gtgtgcggcg tcggcgggca tctcgacggt cgacgaggcg
     9421 gtcgcgttcg tgcgcgagca cggctacccg gtgatcatca agccgcgcgc gggcgccggc
     9481 gcctcgggta cgagccgctg cgacaacgac ggccaactgg cccaggcgct caaggagggc
     9541 cacgtcggcc gcggcggcga ggtcgcgatc gaggagttca tcgagggcca cgagggcttc
     9601 tacgacacga tgtcggtcga cggcgtgccg gtgctcgagt tcgtctcgca ctattatccc
     9661 aatgtgctcg aggcgatgcg tcaccgctgg atctcgccgt acatcgtgtc gaccaaccgc
     9721 ctcgacgcgc ccggctacga cgacctcaag gcgctcggcc gtcgcgtgat cacgacgatg
     9781 ggcctcggca ccaccgcgac ccacatggag tggttctacg ggtcgaaggg cctcaagttc
     9841 tcggagatcg gcgcgcgccc gcccggcgtc ggccagtggg acgtctacgc cgccggcaac
     9901 gacctcgaca tctatcgcga gtgggccaac gcggtcgtgc acggtcgcat cgagcgccag
     9961 ccgtcgcgcc gcttcgccag cggcatcatc gcgctgcgcc ccgaccgcga cggccgcatc
    10021 atcggctacg agggcgtcga ggagatccag cgccgcttcg gcgagtacgt caccaaggcc
    10081 cacctgccgc ccgcaggcac gccgacgcag ggcatcgagg ccggctacat ggccaacgcg
    10141 tggatccaga tgcgccaccc cgactacgac gcgatgcgcg agatcttcga gatcatcggc
    10201 cgcacgatcc gcgtgcgcgc gggctgacca ccttcacccc tctcccccga gcgagcacat
    10261 catgaaagtt ctctttatct ccccgcacta cccggaagag atgcaagact tcacgcgcgg
    10321 cctcgccgag gtcggcgcgc aggtctacgg cgtcggcgac acgccgcgcg gcagcctccc
    10381 gcagcacacg cagcgccacc tgcgtgacta cttgcaggtg ccaggattgt tcgacgagga
    10441 cgaggcgctg cgccgcatcg tcgccgcggc gcgcagcctc aagccggacc gcgtcgagtg
    10501 cctgtgggag ccgtgcatcg tgctcgcggc gcgcgtacgc gaggcgctcg gcatcccggg
    10561 catgcg
//
LOCUS       DASWNJ010000957         2655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_53231, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000957 DASWNJ010000000
VERSION     DASWNJ010000957.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53231"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(490..>2655)
                     /locus_tag="VGB85_16265"
     CDS             complement(490..>2655)
                     /locus_tag="VGB85_16265"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0135640.1"
                     /translation="GYLEDACETYLRWGARASADAIREKHRELFTVATDAASEGKSGA
                     ADILVDTAESKIHGLLDMAAILGATQALAGELDLGALTGRMLTLLVEYAGAERGVLAL
                     ARDGQLHVQAQLRVDLPRLETDLDEPLIDSTRLPTTVVEYVARTSEAVVLGQAVSDNR
                     FADDPYLQAHRPASLLVVPLMHQGRSLGVMYLEHARAVDVFPSERIELVSLLASQAAT
                     AVENATLFGEVQRKTSELAEANAGLEQQVQERTAELRSAKEAADAANRAKSDFLASMS
                     HELRTPLNGVLGHAQILLRSPALAARDRGSVEVIQRSGEHLLTLINDVLDLAKIEAGK
                     FELVPRDVQFAGMLQTVADMCRPRAEQKGLIFTHERVGARLQVIRADEKRLMQVLLNL
                     LGNAIKFTSSGQVVFRVEVDEIEGQGRVCLVRFQVHDTGPGIARQHLGRLFQPFEQQG
                     SSQSRSEGTGLGLAISKRIVDLMDGQIRVDSEPGRGSMFEVEVLLERGASEAPAAPRP
                     GAILGYVGERRRILVVDDVAVNRMVIRGLLEPIGFEVSEASSGEAALELAARVRPALV
                     LMDLAMPGLDGLEAHARMRSIPGLEQTIVIISSARVSEDERARSLAAGCFEFLPKPVH
                     ADRLYELLEQALGVQWQREAPAAAADAGSPAELVVPPAEALRSLQNLAVRGRVRELQD
                     QARALAEGDARLAPWVDRLSTLAQMFQLREVRELIEAGL"
BASE COUNT          398 a          949 c          922 g          386 t
ORIGIN      
        1 cgtttcatgg gtttgaccgt agctagctgc tgtcaattcg accagatcca tcatcgatcg
       61 cccacagcgg cggggatgtg tcggtgagtc gctacattcg ccactcacgg acaccgcgac
      121 gcagatgggc cccgcgggca cacgtgcgag ccggcgaccg acggtgtggt gaatcgcaac
      181 cgcgctcgcg gccaaacgcc gctgcgaccg cttttgcggg catcacgagc cgggcgtaca
      241 caaacagcac acacgcggat cgacggggtc gccgaagcag caccggatca ggtcctgctg
      301 ccacctcgac attgtgagcc cactgcgatc tgtcgcacga gatctctcgt gagagcttgc
      361 aaagcgtgaa gaaaaatatc ccgcgtcacg gcctcgccat gcatcgggtc ggacgctcac
      421 tgggcacaaa tcaatgatgg cacccgtccg aagaccgggg ggcgccgcca tcgatcaggg
      481 tgctgcggac tacagtccag cttcgatcag ctcacggacc tcgcgcagct ggaacatctg
      541 cgcgagcgtg gagagccggt cgacccacgg agccagccgc gcgtcgccct cggcgagcgc
      601 ccgggcctgg tcctgaagct cgcgcacgcg accacgcacg gcgaggttct gcagcgagcg
      661 cagcgcctcc gccggcggga ccaccagctc ggcgggcgac ccggcgtcgg ccgcggccgc
      721 gggggcctcg cgctgccatt gcacgccgag cgcctgctcg agcagctcgt acagccggtc
      781 ggcatggaca ggcttgggca ggaattcgaa gcagccggcg gcgaggctgc gcgcgcgctc
      841 gtcctccgac acccgcgcgg acgagatgat cacgatcgtc tgctcgagcc cggggatcga
      901 ccgcatgcgg gcgtgggcct cgagaccgtc gagccccggc atcgccaggt ccatcaagac
      961 gagggccgga cgcacgcgcg cggccagctc gagcgccgcc tcgccgctgg acgcctcgct
     1021 gacctcgaag ccgatcggct cgagcagtcc gcggatcacc atgcgattga ccgcgacgtc
     1081 gtcgacgacg aggatccgcc tgcgctcgcc gacatagccg aggatcgcgc cgggccgcgg
     1141 cgccgccggg gcctccgagg cgccgcgctc gagcagcacc tcgacctcga acatgctccc
     1201 gcggccaggc tcgctgtcga cgcggatctg gccgtccatc aggtcgacga tccgcttgct
     1261 gatcgccagc ccgaggccgg tgccctcgct gcgcgattga ctgctgcctt gctgctcgaa
     1321 cggctggaac aggcgcccca ggtgctggcg ggcgatgccc ggcccggtgt cgtggacctg
     1381 gaagcggacc aggcacaccc gcccctgccc ctcgatctcg tcgacctcga cgcggaacac
     1441 cacctgcccg ctcgacgtga acttgatcgc gttgccgagc aagttgagca gcacctgcat
     1501 cagccgcttc tcgtcggcgc ggatcacctg caggcgcgcc ccgacccgct cgtgggtgaa
     1561 gatcaggccc ttttgctcgg cccgcgggcg gcacatgtcg gcgaccgtct gcagcatgcc
     1621 ggcgaactgg acgtcgcgcg gcaccagctc gaacttgccg gcctcgatct tggcgaggtc
     1681 gaggacatcg ttgatcaggg tcagcaggtg ctcgccggat cgctggatga cctcgacgct
     1741 gccgcggtcg cgcgcagcga gggccggcga gcgcagcagg atctgcgcgt ggccgagcac
     1801 gccgttgagc ggcgtgcgca gctcgtgact catgctggcg aggaagtcgc tcttggcccg
     1861 gttggccgcg tccgccgcct ccttcgcgct gcgcagctcc gcggtgcgct cctgtacctg
     1921 ctgctcgagg ccggcgttgg cctccgccag ctcggaggtc ttgcgctgca cctcgccgaa
     1981 cagggtcgcg ttctcgacgg cggtcgccgc ctgcgaggcg agcagcgaga ccagctcgat
     2041 gcgctcgctc gggaacacgt cgaccgcccg cgcgtgctcg agatacatga caccgagcga
     2101 ccggccctga tgcatcagcg gcaccaccag cagcgaggcc gggcggtggg cctgaagata
     2161 ggggtcgtcg gcgaagcgat tgtcggacac ggcctgcccg agcacgaccg cctcgctggt
     2221 gcgcgcgacg tactcgacga ccgtcgtcgg caagcgcgtc gagtcgatga gcggctcgtc
     2281 gaggtcggtc tccagccgcg ggaggtcgac gcgcagctgg gcctggacgt gcagctggcc
     2341 gtcgcgcgcc agcgcgagca cgcctcgctc ggcgccggcg tactcgacga gcagcgtcag
     2401 catgcggccg gtcagggcgc cgaggtcgag ctcgccggcg agggcctgcg tcgcgccgag
     2461 gatcgccgcc atgtcgagca gcccgtggat cttgctctcc gcggtgtcga cgaggatgtc
     2521 ggcggcgccc gacttgccct cgctggcggc gtcggtcgcg acggtgaaca gctcgcggtg
     2581 cttctcgcgg atcgcgtccg cgctggcccg cgcgccccag cgcaggtagg tctcgcacgc
     2641 gtcctcgagg taccc
//
LOCUS       DASWNJ010000958         1834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_195107, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000958 DASWNJ010000000
VERSION     DASWNJ010000958.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195107"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VGB85_16270"
     CDS             <1..222
                     /locus_tag="VGB85_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135641.1"
                     /translation="ILWAYDIGQHRLRLLAGAHGQQGLCDGEPALARFWLPTAISLAD
                     DGSGFIVTEAGNRQRRHVSRDGRVRTLPR"
     gene            406..993
                     /locus_tag="VGB85_16275"
     CDS             406..993
                     /locus_tag="VGB85_16275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135642.1"
                     /translation="MGLRNNKHDKHDDDETTSTELAPASVEAEVRRGMNLEHGGETVV
                     EHRGGVDHRHSGMTGFELRGNTHVHHGGGATLELKSDSHIHHDGSAGLELKGSAQVRH
                     DGNVAIGLGGAATVGLAGAAGIQLGNAEGRPLAAAVQLLLGGGNEPVKLGPLSIDLGR
                     VQLQPDLELRFKLFGKYTLFTVNLRGATRLGPDER"
     gene            complement(998..>1834)
                     /locus_tag="VGB85_16280"
     CDS             complement(998..>1834)
                     /locus_tag="VGB85_16280"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0135643.1"
                     /translation="KFIDRRFSLGDDAAVDRVADLVLPLVADDLARFAADYHFICDLS
                     LTEELEFRRTGRYRLSTFAEADREVYSNHAFMTRYMNGLLMSELFWSNHTSVIDTYAR
                     DYLPLQRPGYAHLEIGPGHGLLLSQVAQDERATKISGWDLSPASLDATRHSLDLLGVT
                     REVKLEKRDLFEDADDERFDSIVVSEVLEHMEDPAAALRSLTKRLTPGGRIFVNFPLN
                     SPMPDHLYLLSKPDEAQALVADAGLTIESARFFPMSGYKLERAIKAALTISCVIVGRR
                     EA"
BASE COUNT          287 a          670 c          633 g          244 t
ORIGIN      
        1 atcctgtggg cctacgacat cggccagcac cgcctgcgcc tgctggccgg cgcgcatggt
       61 caacagggcc tctgcgacgg cgagcccgcg ctggcccgct tctggctccc gaccgcgatc
      121 tccctcgccg acgacggctc cggcttcatc gtcaccgagg ccggcaaccg ccagcgccgc
      181 cacgtctctc gcgacggccg cgtccggacc ctgccgcgct gaccacctcg ccacctcgcc
      241 acctcgtgcc gcgacaggcg caggtgcccg gcgagcccgg cgccacccgg agactccccc
      301 catctcccgg cccgccagcg cccacctgcg cccgatctcg cggccgcgcg gccggatcca
      361 gcggcacaac cgccgggcgg cggtaggcct gatgcggagg acgccatggg cctgcgcaac
      421 aacaagcacg acaagcacga cgacgacgaa acgaccagca ccgagctcgc gccggccagc
      481 gtggaggccg aggtgcgacg cggcatgaac ctcgagcacg gcggcgagac ggtcgtcgag
      541 catcgcggcg gcgtcgacca ccgccacagc ggcatgaccg gcttcgagct gcgcggcaac
      601 acccacgtgc atcacggcgg cggcgcgacg ctcgagctca agagcgacag ccacatccat
      661 cacgacggct cggccgggct cgagctcaag ggcagcgccc aggtccgcca cgacggcaac
      721 gtggcgatcg ggctcggcgg cgcggcgacg gtcggcctcg ccggcgccgc ggggatccag
      781 ctcggcaacg ccgagggccg cccgctcgcg gccgccgtgc agctgctgct cggcggcggc
      841 aacgagccgg tcaagctcgg cccgctgtcg atcgacctcg gtcgcgtgca gctgcaaccc
      901 gacctcgagc tgcgcttcaa gctgttcggc aagtacaccc tgttcacggt gaacctgcgc
      961 ggcgcgaccc gcctcggccc cgacgaacgg tagcgcgcta ggcctcgcgg cgaccgacga
     1021 tcacgcagga gatcgtcagc gcggccttga tcgcgcgctc gagcttgtag cccgacatcg
     1081 ggaagaagcg cgcgctctcg atcgtcagcc cggcgtcggc gaccagcgcc tgcgcctcgt
     1141 ccggcttgct cagcaggtac aggtggtccg gcatggggct gttgagcggg aagttgacga
     1201 agatccgccc gcccggtgtc agccgctttg tcaaactgcg cagcgccgcg gccgggtcct
     1261 ccatgtgctc gagcacctcg ctgacgacga tgctgtcgaa gcgctcgtcg tccgcatctt
     1321 caaagaggtc gcgcttctcg agcttcacct cgcgcgtgac gccgagcagg tcgagcgagt
     1381 gacgcgtcgc gtcgaggctc gccgggctga ggtcccaccc ggagatcttg gtggcgcgct
     1441 cgtcctgggc gacctgcgag agcaacaggc cgtgcccggg gccgatctcg aggtgcgcgt
     1501 agccgggcct ttgcagcggc agatagtccc gcgcgtaggt gtcgatcacg ctggtgtggt
     1561 tcgaccagaa cagctccgac atcagcaggc cgttcatgta gcgggtcatg aacgcgtggt
     1621 tcgagtagac ctcgcggtcc gcctccgcga aggtcgagag ccggtaacga cccgtgcggc
     1681 gaaactcgag ctcctccgtc agcgacaggt cgcagatgaa gtggtagtcg gcggcgaagc
     1741 gcgcgaggtc gtcagcgacg agcggcagta cgaggtcggc gacgcggtcg acggccgcgt
     1801 cgtccccgag ggagaaccgg cggtcgatga actt
//
LOCUS       DASWNJ010000959         3883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_329760, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000959 DASWNJ010000000
VERSION     DASWNJ010000959.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329760"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VGB85_16285"
     CDS             <1..360
                     /locus_tag="VGB85_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135644.1"
                     /translation="VFTQVFEVPIYLVALRRRADSPEPLRWRLGFAFLASMATHPYVW
                     FVIPTLFTASAWRAWPALDPWRHALFFVTAETFAVLAEGLLMRALRVPRPFLWALVAN
                     ATSAGLGMLTRALTGWP"
     gene            429..872
                     /locus_tag="VGB85_16290"
     CDS             429..872
                     /locus_tag="VGB85_16290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135645.1"
                     /translation="MWQRDAEFGAPDVVVDAGELAAYRLARMSGRLVQGLSGGGGDPE
                     AVWHALAASCPIDFAISRALAALGRTEPLRGHAIDVRAIRRVQAFAPVEAPSSLCAAA
                     RVLRCNGRPDGDFDAALAVTLTRNPDGAAVASFDLELTLARRAAA"
     gene            complement(877..2673)
                     /gene="argS"
                     /locus_tag="VGB85_16295"
     CDS             complement(877..2673)
                     /gene="argS"
                     /locus_tag="VGB85_16295"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY0135646.1"
                     /translation="MHDASLAAAAARALHPVLPEAAGLTLADVAAAFTTPPDPAMGDL
                     AFPCFKLAKALRKGPPQIAAELATKLASQTGLIAQATAAGPYVNLRLDLGHAGALVLP
                     SLARGETHAPPASGTRVMIEYSQPNTHKAFHVGHLRNLCLGDALVRLLRADGDEVVAA
                     NYLGDVGAHIAKCLWWYLDHLTDRTPPAAHRGEWLGEQYAAASNQLEVWDEQAKAGDA
                     NAAASLAAAKARTTEILQKLEAREPELQKVWDETRQWSLDDFAEIYQWCGVQFDRVFF
                     ESEVDEPGLKLVEEFLERGVFTVSEGAVGVINEEIKHMPFFMLRKRDGTGLYATKDLA
                     LARLKFEEYRIDRSIYVVDVRQSDHFRHVFLTLKKMGFTQADRCQHVPYEMVELPDGP
                     MATRKGNVVLFRSLRQQMTEHIRRAYLDNFKETWPEEEIVETAHAIALGAIKYGMLAR
                     DVNQKIVFDMPAWLELTGNTGPYLQYTAARAQSILNKCAEVDKVLDPAVLAGDAVNTW
                     TEPDPSERALLLALDAMPAAIHGAAASLRPAQLCTYLFDLAQTFNRFNAACPVKPSEG
                     DVLQRRLLLVTATVKAMHHALGLLGIPVPRRM"
     gene            2733..2876
                     /locus_tag="VGB85_16300"
     CDS             2733..2876
                     /locus_tag="VGB85_16300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135647.1"
                     /translation="MGNLYGIVAFALLWLFLASFFFTLFRALLRQSKRRRRGEGADWE
                     HGL"
     gene            complement(2883..3392)
                     /locus_tag="VGB85_16305"
     CDS             complement(2883..3392)
                     /locus_tag="VGB85_16305"
                     /inference="COORDINATES: protein motif:HMM:NF022161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 family protein"
                     /protein_id="HEY0135648.1"
                     /translation="MKKVHLSHEINCSPEVFWKLYFDPVFTEALLREAIKVDDFKVVK
                     MEENDREIVRVTTGKPRISAPAAVQKIIGDNFSYTEEARFDRQTQTLRWKISLSTFTD
                     KTRNEGTMRLEPIGTDRVRRIADAEVEAKIFGMGGMMESGMEKQMTEGWTTGAEFTNR
                     WIAAGKAPA"
     gene            3490..>3883
                     /locus_tag="VGB85_16310"
     CDS             3490..>3883
                     /locus_tag="VGB85_16310"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY0135649.1"
                     /translation="MPASAPEVEALLSRLATLAAEAMPADADPAHDILHVRRVAALAR
                     TISAAEGADPAVAVGAALLHELVNLPKNHPESARSGDLCADAAAELLAREGVEPGRAA
                     AICACIRDHAFSKGVMPASLEARVLQDAD"
BASE COUNT          483 a         1307 c         1443 g          650 t
ORIGIN      
        1 gtgttcaccc aggtcttcga ggtcccgatc tacctcgtcg cgctgcgccg ccgcgcggac
       61 tcgccggagc ccctgcgctg gcgcctcggc ttcgcgttcc tcgcgtcgat ggcgacgcac
      121 ccctacgtgt ggttcgtgat cccgacgctg ttcacggcga gtgcgtggcg cgcctggcca
      181 gcgctggacc cgtggcggca cgcgctgttc ttcgtcaccg ccgagacctt cgccgtgctc
      241 gccgagggcc tgctgatgcg cgcgctccgg gtgccgcggc cgttcctgtg ggcgctcgtg
      301 gccaacgcca ccagcgcggg tctgggcatg ctgacgcgcg cgttgacggg ctggccctga
      361 ggacaggtgt agtcccgggc gcgacctcga gccgtgacag cgccggggcg ccttgcgatg
      421 atgacgggat gtggcagcgt gacgccgagt ttggtgcgcc ggacgtggtg gtggatgcgg
      481 gcgagctggc cgcgtatcgc ctcgcgcgca tgtcgggccg gctggtgcag ggcctgtcgg
      541 gcggtggagg cgatccggag gcggtgtggc acgcgctcgc ggcctcgtgc ccgatcgact
      601 ttgcgatcag tcgggcgctc gcggctctcg gccgcaccga gccgctgcgc ggccacgcga
      661 tcgacgtgcg cgcgatccgg cgggtccagg cgttcgcgcc ggtcgaggcc ccgtcgtcgc
      721 tgtgcgccgc cgccagggtg ctgcgctgca acggccggcc cgacggggac ttcgacgccg
      781 cgctcgccgt caccctgacg cgcaaccccg acggcgccgc cgtggcctcc ttcgacctcg
      841 agctgacgct ggccaggcga gccgcggcct gacggctcac atgcgccgcg gcactgggat
      901 ccccagcagc ccgagcgcgt ggtgcatcgc cttcacggtc gcggtgacca gcagcaggcg
      961 ccgctgcagg acatcgccct cgctcggctt gaccgggcag gcggcgttga agcgattgaa
     1021 ggtctgcgcc aggtcgaaca ggtaggtgca cagctgggcc gggcgcagcg acgcggcggc
     1081 gccgtggatc gcggcgggca tggcatcgag cgcgagcagc agcgcgcgct cgctggggtc
     1141 gggctcggtc cacgtgttga cggcgtcacc ggcgagcacg gcggggtcga ggaccttgtc
     1201 gacctcggcg cacttgttga ggatcgactg ggcgcgggcc gccgtgtact gcaaataggg
     1261 cccggtgttg ccggtcagct cgagccacgc gggcatgtcg aacacgatct tctggttcac
     1321 gtcgcgcgcg agcatcccgt acttgatcgc cccgagagcg atcgcgtggg ccgtctcgac
     1381 gatctcctcc tcgggccagg tctccttgaa attgtcgagg tacgcgcggc ggatgtgctc
     1441 cgtcatctgc tggcgcaggc tgcgaaacag caccacgttg cccttgcggg tcgccatcgg
     1501 cccgtcgggc agctcgacca tttcgtaggg gacgtgctgg cagcgatccg cctgcgtgaa
     1561 gcccatcttt ttaagagtca ggaacacgtg gcggaagtgg tcgctctggc gcacgtcgac
     1621 gacgtagatc gaacggtcga tgcggtactc ctcgaacttg agccgcgcga gggcgaggtc
     1681 cttggtcgcg tacaggcccg tgccgtcgcg cttgcgcagc atgaagaagg gcatgtgctt
     1741 gatctcctcg ttgatgacgc cgacggcgcc ctcgctgacg gtgaacacac cccgctcgag
     1801 aaactcctcg acgagcttga ggccgggctc gtcgacctcg ctctcgaaga agacgcggtc
     1861 gaattgcacg ccgcaccatt ggtaaatttc ggcgaagtcg tcgagcgacc actgccgcgt
     1921 ctcgtcccac accttttgta gctcgggctc gcgcgcctcg agtttttgca ggatctccgt
     1981 cgtgcgggcc ttggcggccg cgagcgacgc ggcggcgttt gcatcgccgg ctttggcctg
     2041 ctcgtcccag acctcgagct ggttgctcgc ggcggcgtat tgctcaccga gccactcacc
     2101 gcggtgcgca gccggggggg tgcggtcggt gaggtggtcg aggtaccacc acaggcactt
     2161 ggcgatgtgc gccccgacat ccccgagata gttggccgcg acgacttcat cgccgtcggc
     2221 gcggagcagg cggacgagcg cgtcgccgag gcacaggttg cgcaggtggc cgacgtggaa
     2281 cgccttgtgg gtgttcggct gcgagtactc gatcatcacc cgcgtgccgc tcgcgggcgg
     2341 cgcgtgggtc tcgccgcggg ccagtgacgg cagcacgagc gcgccagcgt ggccgaggtc
     2401 gaggcgcagg ttgacgtacg gcccggccgc ggtcgcctgc gcgatcagtc cggtctggga
     2461 cgcgagcttc gtcgccagct cggcggcgat ctgcggcggg cccttgcgca gcgccttggc
     2521 cagcttgaag caggggaacg cgaggtcgcc catcgcgggg tcgggcggcg tcgtgaaggc
     2581 cgcggcgaca tcggcgaggg tcaaaccggc ggcctcgggg aggacggggt gcagcgcgcg
     2641 agcggcggcg gcggcgagcg aggcgtcgtg catgggccgg ggagggtagc ccggccgcgc
     2701 gcacgcgggc aatgccgtgc tacaaccgcc gcgtggggaa cctctacggc atcgtcgcct
     2761 tcgcgctgct ctggctgttt ctggcgagct tcttcttcac gctgtttcgg gccctgctgc
     2821 gccagtcgaa gcgccgccgc cgtggcgagg gcgccgactg ggagcacggg ctgtagtcgg
     2881 cttcaggccg gggctttgcc ggcggcgatc caccggttgg tgaactcggc cccggtggtc
     2941 cagccctcgg tcatttgctt ctccatgccg gactccatca tcccgcccat gccgaagatc
     3001 ttggcctcga cctcggcgtc ggcgatgcgg cgcacgcggt cggtgccgat cggctcgagg
     3061 cgcatggtgc cctcgttgcg ggtcttgtcg gtgaacgtgc tgaggctgat cttccagcgc
     3121 agggtctggg tctggcggtc gaagcgggcc tcctcggtgt agctgaagtt gtcgccgatg
     3181 atcttctgca ccgcggcggg cgcggagatc cgcggcttgc cggtggtgac gcggacgatc
     3241 tcgcggtcgt tctcctccat cttcacgacc ttgaagtcgt cgaccttgat cgcctcgcgc
     3301 agcagcgcct cggtgaagac cgggtcgaag tacagcttcc agaacacctc gggggagcag
     3361 ttgatctcgt gggacaggtg gactttcttc atggcgcggc cgcgaggata gcggatcggg
     3421 gcgcgcgcgg gggcgcgccg gcgcgggcgg gtgcgcggcg ggccatgtcg cgctacacct
     3481 gcgcctcgca tgcccgcgtc cgcccccgaa gtcgaggccc tgctctcacg tctcgcgacc
     3541 ctcgccgccg aggcgatgcc ggccgacgcc gacccggcgc acgacatctt gcacgtgcgt
     3601 cgcgtcgccg cgctcgcgcg cacgatctcc gcggcggagg gcgccgatcc ggccgtcgcg
     3661 gtcggagcgg cgctgctgca cgagctcgtc aacttgccga agaaccaccc ggagtcggcg
     3721 cgctcggggg acctgtgcgc cgacgcggcg gccgagctgc tcgcgcgtga gggcgtcgag
     3781 ccgggccgcg ccgcggcgat ctgcgcgtgc atccgcgatc atgcgttttc gaagggggtg
     3841 atgccggcct cgctcgaggc gcgggtgctg caggacgccg acc
//
LOCUS       DASWNJ010000960         1544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_654073, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000960 DASWNJ010000000
VERSION     DASWNJ010000960.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654073"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..477
                     /locus_tag="VGB85_16315"
     CDS             268..477
                     /locus_tag="VGB85_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135650.1"
                     /translation="MTGRTRHFLLLNLCMCMSLLRCTPAIAERDESAVGRVPAALQRW
                     IARASEAGRPVVRPLQALRAVAGVL"
     gene            complement(483..1211)
                     /locus_tag="VGB85_16320"
     CDS             complement(483..1211)
                     /locus_tag="VGB85_16320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135651.1"
                     /translation="MANFGPDMLSRFTAALAAVDRDTDALAAALKLFDRFQDLDLHKP
                     RVAAKLPAIEHCPAGARYRLLVDCESGDPKYWMEIGSDWQVHARRDEPAPVDLAPGDV
                     VLRPPSTAPAPPRSTAGTLAHPLRLCRAWIDECKAARQVAHRAHDLDEASRLAQLAFE
                     LADARTLLTLLAGVDVTAVERATTIPYYQCGYYDVHLAIDMASSDWSRWVEVRRDGPP
                     CPLGEARGHEYDIFSGHLVLRPAR"
     gene            complement(1221..>1544)
                     /locus_tag="VGB85_16325"
     CDS             complement(1221..>1544)
                     /locus_tag="VGB85_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135652.1"
                     /translation="EPTADTAEPASGELPEQLSAQEFRRIMLRSNRTTQVRTCYRRHA
                     RPGEEDVSLIGIVSAAGKIQKLRMEPAIPLTECLKPIVQKIEFPQAQRPAQHNFIYRN
                     MFPDG"
BASE COUNT          210 a          476 c          617 g          241 t
ORIGIN      
        1 gccgtcgcgg aggtgcaagg tgtcgcggcg ggtacggagc tctgcggggg ccttggccat
       61 tgggactgtt tcggcgtcac gcgcgcgagg ccaggccccg gtcgcaggcg aggatagcat
      121 cgccctcacg tccggggccg cggtcgaacc cccgaaatgt ccggatccgg gaccggccgt
      181 cccggatccg gtcaccggag cgcgacgtga tcgtgtcgga tcaccggggt gcgggcggca
      241 tgcttcttgc gatgagtccg gcgaaccatg acggggcgaa ccaggcattt tctcttatta
      301 aatctatgca tgtgcatgtc gctgctgcgc tgcaccccgg cgatcgcgga gcgcgacgag
      361 tcggcggtcg gcagggtgcc ggcggcgctg cagcgatgga tcgcacgggc gagcgaggcg
      421 gggcggcccg tcgtgcgccc gctgcaggcg ctgcgggcgg tcgcgggggt gctgtgatcg
      481 ggctaccgcg cggggcggag cacgaggtgg ccggagaaga tgtcgtactc gtggccgcgc
      541 gcctcgccga gcgggcaggg ggggccgtcg cggcggacct cgacccagcg tgaccagtcg
      601 gacgaggcca tgtcgatcgc caggtgcacg tcgtagtatc cacattgata ataggggatc
      661 gtggtcgccc gctcgacggc ggtgacgtcg acgccggcga gcagcgtcag gagagtgcgg
      721 gcgtcggcca gctcgaacgc caattgagcc aggcggctgg cctcgtcgag gtcgtgggcc
      781 cggtgggcga cctggcgggc ggccttgcac tcgtcgatcc aggcgcgaca caggcgcagc
      841 ggatgcgcga gcgtaccggc ggtcgagcgt gggggcgcgg gcgcggtgga cggtgggcgc
      901 aggacgacgt ccccgggggc gaggtcgacg ggcgcgggct cgtcgcgccg ggcatgaacc
      961 tgccagtcgg acccgatctc catccagtac ttcgggtcgc cggattcaca gtcgacgagc
     1021 aggcggtagc gtgcgcctgc ggggcagtgt tcgatggccg gtaacttcgc ggcgacccgc
     1081 ggtttgtgca ggtcgaggtc ctggaatcga tcgaagagct tcaaggcggc cgcgagcgcg
     1141 tccgtatcgc ggtccaccgc ggcgagcgcg gcggtgaagc gggacagcat gtccgggccg
     1201 aagttggcca tcgccggacc ctagccgtcg gggaacatgt tgcgatagat gaagttgtgc
     1261 tgggcgggcc gctgcgcctg cgggaactcg atcttctgca cgatcggctt gaggcactcg
     1321 gtgagcggga tcgccggctc catgcgcagc ttctggatct tgccggccgc gctgacgatg
     1381 ccgatcagcg agacgtcctc ctcgcccgga cgcgcgtgcc ggcggtagca ggtgcgcacc
     1441 tgggtggtgc ggttgctgcg aagcatgatg cggcggaact cctgcgcgct cagctgctcc
     1501 ggtagctcgc cgctcgcggg ctcggcggtg tcggcggtcg gctc
//
LOCUS       DASWNJ010000961         2692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_400255, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000961 DASWNJ010000000
VERSION     DASWNJ010000961.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400255"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VGB85_16330"
     CDS             complement(<1..188)
                     /locus_tag="VGB85_16330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0135653.1"
                     /translation="MTTPKEGQAELRAESSARQTAETALAASEEQFRMLINGVRDYAI
                     FLLDPNGYVVSWNPGAER"
     gene            467..817
                     /locus_tag="VGB85_16335"
     CDS             467..817
                     /locus_tag="VGB85_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135654.1"
                     /translation="MANNLTGLMCVVFACTLSACDGDDAAIVAGATAMPAARTLEDSK
                     TARRDTTAPADVVEPRVQLAAVLAREISAAPEQSSKILRRHGLDRDKLDTIMFEIAGD
                     PELTRAYLVARRSR"
     gene            complement(855..1514)
                     /locus_tag="VGB85_16340"
     CDS             complement(855..1514)
                     /locus_tag="VGB85_16340"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY0135655.1"
                     /translation="MKVFSNTAISLDGRINTREGRFTTLGTARDHARMSRLRGQADAV
                     LVGGATFRNWPHPSLPEGDDLLGLPAPFYNVIVTRSLEVPVPAELLAEPRIRPLFLTR
                     DGVVPAGFPAEYEAYTGPGDSLPISWILQVLRRRGVQRLLIEAGGDLLFQFLEADAID
                     EINVTLCPLVIGGPTPSLASGRGFDFAEMPRLQLMSSEVEGDEIFLRYAVRRNDAAPQ
                     G"
     gene            complement(1511..1912)
                     /locus_tag="VGB85_16345"
     CDS             complement(1511..1912)
                     /locus_tag="VGB85_16345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEY0135656.1"
                     /translation="MTAPPTGRTLAKDPSCMDFLCLEGLEVPCRVGCTDIERATLQSL
                     RVDVRLYCEDLQRAGQDDDLAATVDYRVATEMIAAVQDHEYLLIERVAEVLADVALRH
                     PQVTRVQISVRKRPPVQGLEIAGVQIHRTRA"
     gene            complement(2043..>2692)
                     /locus_tag="VGB85_16350"
     CDS             complement(2043..>2692)
                     /locus_tag="VGB85_16350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0135657.1"
                     /translation="GGALAMIVHDLRTPLGNIQGLCDMIDEAVAVGHTDEVREYVPHV
                     RESSHEAMSIVNDVLDFTREAAMERVSVRLGELARQLRDKTRHLFPSDALEVLVPTDE
                     VVSVDAPRLQRGLVNLIKNACEALAKQRTPAPRVTATLGLDGEDAVFIVADNGPGIAA
                     ELTSRLFEPFATHGKSGGTGLGLAIVKRIAESHGGTLTVASSPDGATFTVRVPRA"
BASE COUNT          406 a          920 c          953 g          413 t
ORIGIN      
        1 atccgctcgg cgccggggtt ccagctgacc acgtagccgt tcgggtcgag cagaaagatc
       61 gcgtagtccc ggacgccgtt gatcagcatc cggaactgct cctcgctcgc tgcgagggcc
      121 gtctccgcgg tctgtcgggc gctggactcc gcgcggagct ccgcctggcc ttccttcggg
      181 gtcgtcaccg tacgttcgct cccaccttgc gctgttgccg cagttgggtg gtgttgtagc
      241 attggccggg agcgcaacgg atgcgccgcc gctggcaagc cgccacgggg cgggcaggag
      301 tcaaggtctg caggagacgg ttcggtgcgc agtccccgaa aattccctgc cgggcccggc
      361 gccggttcgg gcctcgtcgt ccgcattttt accggccgct gtaatcctcg ccaggcccgg
      421 gtcactcatc acagtcgcag cgaatgcgac atctggagtc gaaccgatgg cgaacaatct
      481 cactggcctg atgtgcgtcg tctttgcatg cacgctgtcc gcctgcgacg gcgacgacgc
      541 tgcgatcgtc gccggggcga ccgcgatgcc ggcggcgagg accctcgagg actcgaagac
      601 agcgaggcga gacacgactg ccccggccga cgtggtggag cccagggttc agctcgccgc
      661 cgtgctcgcc cgagagatct ccgcggcgcc cgagcagtcg agcaagatcc tgcgcaggca
      721 cggcctcgac cgcgacaagc tcgacacgat catgttcgag atcgccggtg accccgagct
      781 gacgcgggca tacctggtcg cgcgtcgaag cagatgaagc agatgaagca gatgaagcag
      841 ataacgcggc gccgtcaccc ctgcggagcg gcgtcattgc gccgtaccgc atatcgcagg
      901 aagatctcgt cgccctcgac ctcggaggac atgagctgca ggcggggcat ctcggcgaag
      961 tcgaagcccc ggccgctcgc cagcgacggg gtcggcccgc cgatgacgag cggacacagg
     1021 gtcacgttga tctcgtcgat cgcgtcggcc tcgaggaatt gaaacagcag gtcgccgccc
     1081 gcctcgatca ggaggcgctg caccccgcgg cggcgcagca cctgcaagat ccacgaaatc
     1141 ggcagcgagt cgcccggccc cgtgtacgcc tcgtactcgg ccggaaaccc cgcgggcacg
     1201 accccgtccc gggtcaggaa cagcgggcga atgcgcggct cggcgagcag ctcggcgggc
     1261 actgggacct cgaggctccg cgtgacgatc acattgtaaa aaggcgcggg cagaccgagc
     1321 aggtcgtcgc cctcgggcag cgaggggtgg ggccagttgc gaaaggtcgc gccgccgacg
     1381 agcacggcgt ctgcctgacc gcgcagccgg ctcatgcgcg cgtggtccct ggcggtgccg
     1441 agcgtggtaa atcgcccctc gcgggtgttg atccgcccgt cgagcgagat cgcggtgttg
     1501 ctaaagacct tcatgcgcgt gtgcgatgga tctggacccc ggcgatctcg aggccctgga
     1561 cgggcggccg cttgcgcacc gagatctgca cgcgggtgac ctgggggtgc cgcagcgcga
     1621 cgtcggcgag cacctcggcg acccgctcga tcagcaggta ctcgtggtcc tgcacggcgg
     1681 cgatcatttc ggtcgctacg cgatagtcga cggtggccgc gaggtcgtcg tcctgtccgg
     1741 cgcgctgcag gtcctcgcag tacagccgca catcgacccg cagcgactgc agcgtcgccc
     1801 gctcgatgtc cgtgcagccg acgcggcagg ggacctcgag gccctcgagg cacaggaagt
     1861 ccatgcagga cggatccttg gcgagcgttc ggccggtggg tggggcggtc atgcctgtgt
     1921 tcgtgcctgt tcggggtgcg cagcccgggg gagagatcgg ttagtagtat gactgcgaca
     1981 ccggggcaag cctcgcgggc cgatctcgcc ggacgggccc acgaacacgg gcggcggtcg
     2041 cgtcaggctc tcggcacgcg caccgtgaac gtcgcgccgt ccggcgagct ggcgaccgtc
     2101 agcgtcccgc cgtgggactc cgcgatccgc ttgacgatcg ccaggcccag gcccgtgccg
     2161 ccgctcttgc cgtgggtcgc gaacggctcg aacagccggg acgtcagctc ggccgcgatg
     2221 cccggtccgt tgtcggcgac gatgaacacg gcgtcctcgc cgtcgaggcc cagcgtcgcc
     2281 gtgacccgcg gcgcgggcgt gcgctgcttc gccagcgcct cgcaggcgtt cttgatcagg
     2341 ttgacgagcc cgcgctggag tctgggcgcg tcgaccgaga ccacctcgtc ggtcggcacg
     2401 agcacctcga gcgcgtcgga cgggaacagg tggcgcgtct tgtcacggag ctggcgcgcg
     2461 agctcgccga ggcgcaccga tacgcgctcc atcgcggcct cgcgcgtgaa gtcgaggacg
     2521 tcgttgacga tgctcatcgc ctcgtggctc gactcgcgca cgtgcggcac gtactcgcgc
     2581 acctcgtcgg tgtggccgac ggcgaccgcc tcgtcgatca tgtcgcacag cccctggatg
     2641 ttgcccagcg gggtgcgcag gtcgtgcacg atcatcgcca gcgcgccgcc ca
//
LOCUS       DASWNJ010000962         1594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_510441, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000962 DASWNJ010000000
VERSION     DASWNJ010000962.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510441"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /gene="htpG"
                     /locus_tag="VGB85_16355"
     CDS             <1..1168
                     /gene="htpG"
                     /locus_tag="VGB85_16355"
                     /inference="COORDINATES: protein motif:HMM:NF012410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEY0135658.1"
                     /translation="KPEDYKEFYKHVMGGFVLPGDEPLGHLHVSADAPIQYVALLYIP
                     GKPPADMFQEDRKSLQLFARRVLIIDNCDKLLPAYLRFMRGIVDSEDLPLNVSREMLQ
                     EHNTLSAIRRQLTRKVFKLLADIASERPDDYTKVWDNFGAVLKEGLHTDHSQQDEIMN
                     LLRFRSSQSDGKLVSLADYVASMPEGQEAIYYITGESLEAVARSPHLEACTSRGYQVL
                     FMTDPIDEWVVQDLPNFKDKPLRSVTQGDLDIPEKDDDKKDDSKTDIAPALGKARELL
                     SDRVKDVRASKRLRESASCLVDDEGGLSRNMERILKAARQNVPTRQRILELNPDHPFV
                     QAVSKLASEAPSDERVGTWLELLYDQAALAEGVVADPAGMVKRIQSVLNSAIKV"
     gene            1305..>1594
                     /locus_tag="VGB85_16360"
     CDS             1305..>1594
                     /locus_tag="VGB85_16360"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0135659.1"
                     /translation="MSWEPATYEQFKAERAQPFHDLLALVEPQPDMDVVDLGCGTGEL
                     TTLLHEHLWARSTLGLDSSPTMLAHTCSEGQVPMPLTPAILDATGLRFALQD"
BASE COUNT          286 a          618 c          470 g          220 t
ORIGIN      
        1 caagccggag gactacaagg agttctacaa gcacgtcatg ggcggcttcg tgctgccggg
       61 cgacgagccg ctcggccact tacacgtcag cgccgacgcc ccgatccagt acgtcgcgct
      121 gctctacatc ccgggcaagc cgccggccga tatgttccag gaggaccgca agagcctcca
      181 gctgttcgcg cgtcgcgtcc tgatcatcga caactgcgac aagctgctgc cggcgtacct
      241 ccgcttcatg cgcggcatcg tcgactccga ggacctgccg ctcaacgtct cgcgcgagat
      301 gttgcaggag cacaacaccc tcagcgcgat ccgccgccag ctgacccgca aggtgttcaa
      361 gctgctcgcc gacatcgcca gcgagcgccc cgacgactac accaaggtgt gggacaactt
      421 cggcgccgtc ctcaaggagg gcctgcacac cgaccacagc cagcaggacg agatcatgaa
      481 cctgctgcgc tttcggagct cgcagagcga cggcaagctc gtctcgctcg ccgactacgt
      541 cgccagcatg cccgagggcc aggaggcgat ctactacatc accggcgagt cgctcgaggc
      601 cgtcgcccgc agcccgcacc tcgaggcttg cacctcgcgc ggctatcaag tcctcttcat
      661 gaccgacccg atcgacgagt gggtcgtgca ggacctgccg aacttcaagg acaagccgct
      721 tcgcagcgtc acccagggtg acctcgacat ccccgagaag gacgacgaca aaaaggacga
      781 cagcaagacc gacatcgccc ccgcgctcgg caaggcccgc gagctgctct cggaccgcgt
      841 caaggatgtc cgcgccagca agcgcctgcg tgagtccgcc tcctgcctcg tcgacgacga
      901 gggcggcctg tcgcgcaaca tggagcggat cctcaaggcc gcgcgccaga acgtcccgac
      961 ccgccagcgg atcctcgagc tcaacccgga ccacccgttc gtccaggccg tgagcaagct
     1021 ggccagcgag gccccgtccg acgagcgcgt cggcacctgg ctcgagctgc tctacgacca
     1081 ggccgccctc gccgagggcg tcgtcgccga ccccgccggc atggtcaagc gcatccagtc
     1141 cgtcctcaac agcgcgatca aggtctaaag gcctcaccgc gaacacgcac gagccgccga
     1201 tccaccgctt ccgcggggga cggcggctct tcgcgttgcg gccgccgtcg cagcggccca
     1261 ccccgaccct tgccaggacc ccggccctgc ggtctcatcc ccccatgagc tgggagcccg
     1321 ccacctacga gcagttcaag gccgaacgcg cccaaccctt ccatgacctg ctcgcgctcg
     1381 tcgagcccca gcccgacatg gacgtcgtcg acctcggctg cggcaccggc gagctgacca
     1441 ccctgctcca cgagcacctg tgggcccgct cgaccctcgg cctcgacagc tcgccgacga
     1501 tgctcgcgca cacctgctcc gagggacagg tgccgatgcc gctgacccct gcgatcctcg
     1561 acgcgaccgg cctgcgcttc gcgctgcagg acat
//
LOCUS       DASWNJ010000963         2401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_229397, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000963 DASWNJ010000000
VERSION     DASWNJ010000963.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229397"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VGB85_16365"
     CDS             complement(<1..363)
                     /locus_tag="VGB85_16365"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY0135660.1"
                     /translation="MPRLAVRAILIEDDRILLAHFVDAGGPWYVIPGGGVQDGETLEE
                     AFHRELAEEIGARAEFGAVAFIREIIADRLETTNLPAGFHQLEIFVHGKLLPDQTLRM
                     VEPDAAQVGLVWVPLADLH"
     gene            complement(366..1796)
                     /locus_tag="VGB85_16370"
     CDS             complement(366..1796)
                     /locus_tag="VGB85_16370"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0135661.1"
                     /translation="MARSEYDAVIVGAGPNGLSAAIELGRAGLSTLVVEAGATPGGGC
                     RSGERTIPGFVHDHCAAVHPLGASSPLFRELGLERHGLQWVHSPALLAHIVGEGNVMT
                     LERSLDDAAAVFGRDGRAYRKLVAPFVERWDELYPMVLGPLRFPSKPILMGRFGLQAL
                     RSMRGLAHGRFEQESVRALLAGISGHAMVPLDSLATASFALVLAIAGHAGGWPVARGG
                     SQAIINALVACMREQGGELELGHTVTNIAELPKARAYLFDVTPRQLLAIAGDRLPAGY
                     RERLASFRYGPGVYKLDWALSGPIPWRDPACARAATVHLSGTFDDIEVAMKAIHAGTI
                     SDRPFVLLAQPSLFDDTRAPAGKHTAWAYCHVPHGSTLDASDAIEGHIERYAPGFRDL
                     IIGRSTKNAVEMQQYNANYVGGDINGGYAELSQLFFRPVARYDPYSTPAKDIYICSSS
                     TPPGGAVHGMCGYWSARSALRRVFGK"
     gene            complement(1769..>2401)
                     /locus_tag="VGB85_16375"
     CDS             complement(1769..>2401)
                     /locus_tag="VGB85_16375"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0135662.1"
                     /translation="HCARAVVPVFRRQRRGTMINVGSVLGKVGNPFVPSYTISKFGLA
                     GLSESLRVELAEHPDIHICTLYPYAIDTPHFEDGANALGLQANAMPPMQSPETIARAI
                     VGLARWPRRQRFVPRIAALGLAVHWMFPRLTERLLLRALWRFHLGRVEPTTDGNLFEP
                     ASGEATVHGHRPPRISTPAFVFWSAREILRIGVQAAREQVQRWREANTTQ"
BASE COUNT          385 a          825 c          866 g          325 t
ORIGIN      
        1 gtgcaggtcc gccagcggta cccagacgag gccgacctgc gcggcgtcgg gctcgaccat
       61 gcgcagggtc tgatcgggca gcagttttcc gtgcacgaag atctcgagct gatgaaagcc
      121 ggccgggagg ttggtggtct cgaggcggtc ggcgatgatc tcgcggatga aggcgaccgc
      181 gccaaactcc gcgcgggcgc cgatctcctc ggcgagctcg cggtgaaacg cctcctcgag
      241 ggtctcaccg tcctgcacgc cgccgcccgg gatgacatac caggggccac ccgcgtcgac
      301 gaagtgcgcg agcaggatcc ggtcgtcctc gatgaggatc gcgcgcaccg cgaggcgtgg
      361 catgcctact tgccgaacac ccgccgcagc gcgctgcggg ccgaccagta gccgcacatg
      421 ccgtgcacgg cgccgcccgg cggggtcgac gacgaacata tataaatgtc cttcgccggg
      481 gtcgagtacg gatcgtaacg cgcgaccgga cgaaagaaca gctggctgag ctcggcgtaa
      541 ccaccgttga tgtcgccgcc gacgtagttc gcgttgtact gctgcatctc gaccgcgttc
      601 ttggtcgagc ggccgatgat caggtcgcga aagccggggg cgtagcgctc gatgtgcccc
      661 tcgatcgcgt cgctcgcgtc gagggtcgag ccgtgcggga cgtggcagta ggcccacgcg
      721 gtgtgcttgc cggcgggcgc ccgtgtgtcg tcgaacaggc tcggctgggc cagcaacacg
      781 aacgggcggt cggagatcgt gccggcgtgg atcgccttca tcgcgacctc gatgtcgtcg
      841 aaggtgcccg agaggtgcac ggtcgccgcg cgggcgcacg ccgggtcgcg ccacgggatc
      901 gggccgctga gcgcccagtc gagcttgtat acgccggggc cgtaacgaaa cgaggccagc
      961 cgctcgcgat acccggccgg cagacgatcg cctgcgatcg ccagcagctg ccgcggcgtg
     1021 acgtcgaaca ggtaggcgcg cgccttcggc agctcggcga tgttggtgac cgtgtggccg
     1081 agctcaagtt cgccgccctg ctcgcgcatg caggcgacca gcgcgttgat gatcgcctgc
     1141 gagccgcccc gcgcgaccgg ccagccgccg gcgtggccgg cgatcgccag gaccagcgcg
     1201 aacgaggcgg tcgccagcga gtcgagcggg accatcgcgt gccccgagat gcccgccagc
     1261 agcgcgcgca ccgactcctg ctcgaagcgc ccgtgggcga ggccccgcat cgaacgcagg
     1321 gcctgcagac cgaagcgacc catcaagatc ggcttgctcg ggaagcgcag cgggccgagc
     1381 accatcggat acagctcgtc ccagcgctcg acgaatggcg cgacgagctt gcgatatgcg
     1441 cggccgtcgc gaccgaagac cgcggccgcg tcgtcgagcg agcgctcgag ggtcatgaca
     1501 ttgccctcgc cgacgatatg cgcgagcagg gccggcgagt gcacccattg caggccgtgg
     1561 cgctcgagcc cgagctcgcg aaacagcggc gagctggcgc cgagcgggtg gaccgcggcg
     1621 cagtggtcgt ggacaaagcc ggggatcgtg cgctcgccgg agcgacagcc accgccgggg
     1681 gtcgcgccgg cctcgaccac gagggtcgac aggccggccc ggccgagctc gatcgccgcg
     1741 ctgaggccgt tggggccggc accgacgatt actgcgtcgt attcgcttcg cgccatcgtt
     1801 gcacctgttc gcgggcagcc tgaacgccga tccgaaggat ctcccgcgcc gaccagaaga
     1861 cgaacgcggg tgtgctgatc cggggcggcc ggtgcccgtg cacggtcgcc tcgccggacg
     1921 cgggctcgaa caaattaccg tcggtggtcg gctcgacccg cccgaggtga aagcgccaca
     1981 gcgcgcgcag cagcagccgc tccgtcagcc gcgggaacat ccagtgcacc gcgagcccga
     2041 gcgccgcgat cctcggcacg aagcgctggc gtcgcggcca cctcgccagc ccgacgatcg
     2101 cccgcgcgat cgtctcaggg gactgcatcg gcggcatcgc gttggcctgg agaccgagcg
     2161 cgttggcgcc gtcctcgaag tgcggggtgt cgatcgcgta cgggtagagc gtgcagatat
     2221 gaatgtcggg gtgctcggcc agctcgacgc gcagcgactc cgacagcccg gcgaggccga
     2281 acttgctgat cgtgtacgac ggcacgaaag gattgccgac cttgcccagc accgagccga
     2341 cgttgatcat cgtgccgcgc cgctgccggc gaaacaccgg cacgaccgcc cgcgcgcagt
     2401 g
//
LOCUS       DASWNJ010000964         1537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_688243, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000964 DASWNJ010000000
VERSION     DASWNJ010000964.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688243"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /locus_tag="VGB85_16380"
     CDS             <1..1251
                     /locus_tag="VGB85_16380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135663.1"
                     /translation="KLHIQQQAWTAANAEFTALRRDFDARTKALAGALSVEADAAAYY
                     AAVARNEGPDFKLDALLPRGALGLARGLRRAAQAENLARETGAVSRMLGETRELLARM
                     ETAAQAPERPRLFTDLGAHWTSIDQAGFELVDAAEQMLTRASARLDPRSVRELEARRR
                     EQREALDALRSGSSLRARRITGLGELLREVDGESARLRAQVLGLEQGQLGSGRPRGPS
                     FFTEAAAVRGELAESEAKASDLRARLIHARSTLRFTDPLLTTRRAALVAYRAYLTGAL
                     DAAARAAGDPAINSLLIRMRRVDTRLEAARTKLELAANRRLTDALVILREERENLDRY
                     LGELSELGERAYATIGQATAAAVRDLGGELKYWTTRSEVGQLDVAWAVQHSEQREAEQ
                     LERTRDQSLRELDRALDQVMEEIE"
     gene            1248..>1537
                     /locus_tag="VGB85_16385"
     CDS             1248..>1537
                     /locus_tag="VGB85_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135664.1"
                     /translation="MKDLSKWTCPIGLVVAFAASPAAALPRSDLPRAEDMWQGLRGEP
                     GSGAAREPGSGDPARLTTPPSRQAQNPEDAAALAAMETITARYRNAARISAHT"
BASE COUNT          221 a          637 c          511 g          168 t
ORIGIN      
        1 aagctgcaca ttcaacagca ggcctggacg gcagccaacg ccgagttcac cgccctgcgc
       61 cgcgacttcg acgcccgcac gaaggccctc gccggggccc tctccgtcga ggccgacgcc
      121 gccgcctact acgccgccgt cgcccgcaac gagggccccg acttcaagct cgacgccctg
      181 ctcccgcgcg gcgccctcgg gctcgcccgc ggcctgcgtc gcgcggccca ggccgagaac
      241 ctggcccgag agacaggtgc cgtctcccgc atgctcggcg agacccgcga gctgctcgcg
      301 cgcatggaga ccgccgcgca ggcgcccgag cgcccgcgcc tgttcaccga cctcggcgcc
      361 cactggacct cgatcgatca ggccggcttc gagctcgtcg acgccgccga gcaaatgctg
      421 acccgcgcga gcgccaggct cgaccctcgc agcgtccgcg agctcgaggc ccggcgccgc
      481 gagcaacgcg aggccctcga cgccctgcgc agcggcagct ccctgcgcgc gcggcggatc
      541 accggcctcg gcgagctgct gcgcgaggtc gacggggaat cggcccgcct gcgcgcccag
      601 gtcctcggcc tcgagcaggg ccagctcggc tcgggtcgcc cacgcgggcc cagcttcttc
      661 accgaagccg ccgccgtgcg cggcgagctg gccgagagcg aggccaaggc gagcgacctg
      721 cgcgcccgcc tgatccacgc gcgcagcact ttgcgcttca ccgacccgct gctgaccacc
      781 cgccgcgccg cgctcgtcgc ctaccgcgcc tacctcaccg gcgccctcga tgccgcggcg
      841 agggccgccg gcgaccccgc gatcaacagc ctgctgatcc gcatgcgtcg cgtcgacact
      901 cgcctcgagg ccgcccgcac caagctcgag ctcgccgcca accgtcgcct cacggacgcg
      961 ctggtgatcc tccgcgagga gcgcgagaac ctcgatcgtt acctcggtga attgagcgag
     1021 ctcggcgagc gcgcctacgc gacgatcggc caggccaccg cggccgccgt gcgcgacctc
     1081 ggcggcgagc tcaagtactg gaccacccgc agcgaggtcg gccagctcga cgtcgcctgg
     1141 gccgtgcagc acagcgagca acgcgaggcc gagcagctcg agcgcacccg cgaccagagc
     1201 ctgcgcgagc tcgaccgcgc cctcgaccag gtgatggagg agatcgagtg aaggacctgt
     1261 cgaaatggac atgtccgatc ggtctggtcg tcgcgttcgc ggcgagccct gccgccgcct
     1321 tgccgcgctc cgacctgccg cgcgccgagg acatgtggca gggcctgcgc ggcgagccgg
     1381 gctcgggcgc tgcgcgcgag ccgggctccg gcgaccccgc gcgcctgacc acgccaccat
     1441 cgcgccaggc ccagaacccc gaggacgcgg ccgccctcgc ggcgatggag acgatcaccg
     1501 cccgctaccg caacgccgcc cgcatcagcg cccacac
//
LOCUS       DASWNJ010000965         2057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_18162, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000965 DASWNJ010000000
VERSION     DASWNJ010000965.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18162"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2024)
                     /locus_tag="VGB85_16390"
     CDS             complement(<1..2024)
                     /locus_tag="VGB85_16390"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEY0135665.1"
                     /translation="MATTDTVVVSGAEDATVRFWDVASGRALQVGEAHREHLRTVAIS
                     PNGRHAASSGLDNQLLLWDVASGELARTIYDAGANVVRIPGFGNLYIATGNDSGKGHH
                     DAARRLLFLDGGETLASIEKDVIFWDVATGEEQRRWPRWGWEHEAVAVHPEGRLLVLA
                     GNGYMQVRELATGALVTTLGCGGEHVTALSFSPNGRWLLAGTNEGAVEVWDFDQGLRA
                     GPGGPHQHAINKLETAGDRALSVDTGGGVVLWDLEVPGPLRALDVEHEANGCALGLGE
                     GMALTGEARGFAVWDAQTGALRRRVQCAPTENSHNPHALAPVAGERALVGFLGEGLAL
                     YDLADGGEEHALAGKTQQVSVLAVSPDGRHAASTGYFERAADRARASKKDRGDVSSVM
                     HLQGWDLETRSLLWTVASDGGPDVWIDFGFCLYTPDGRLVTESSESDRVLAFWDARTG
                     AVLQTVELPGQNPMAARMVGRDLVLLVFDDDRTEEGSSCTAVTIDLATAEITQQRSLG
                     RLHCSTASFAPDGSRVAIASPSVLDVYDAASGERVARHECGSDVRTMSFTDNGHILVG
                     DQGGRVHVLALDGWQPSPVRGWTPEEIAELQRETAAREPVRAAPAAKQAVVKKKKTVA
                     KPAAKKAAKKPAKSAAKKPAKSAAKKPAKKKPAKSAAKKPAKSAAKKPAK"
BASE COUNT          265 a          817 c          644 g          331 t
ORIGIN      
        1 ctcttcgcgg gcttcttcgc cgcactcttc gcgggcttct tcgccgcact cttcgcgggc
       61 ttcttcttcg cgggcttctt cgccgcactc ttcgcgggct tcttcgccgc actcttcgcg
      121 ggcttcttcg ccgctttttt cgcggcgggc ttcgccacag tcttcttctt cttcacgacc
      181 gcctgcttcg ccgcgggcgc agcccgcacc ggctctcgcg ctgcggtctc ccgctgcagc
      241 tcggcgatct cttcgggcgt ccacccgcgc acggggctcg gctgccagcc gtcgagcgcg
      301 agcacatgca cgcgaccgcc ctggtccccg accaaaatgt gtccattgtc cgtaaagctc
      361 atcgtgcgca cgtcggaccc gcactcgtgg cgtgcgacgc gctcacccga ggccgcgtcg
      421 tagacatcca gcacgctggg cgacgcgatc gccacgcgcg agccgtccgg ggcgaacgac
      481 gcggtgctgc aatggagccg tccgagcgat cgttgctgcg tgatctcggc ggtggcgagg
      541 tcgatcgtca ctgctgtgca ggacgagccc tcctcggtcc tgtcgtcgtc gaagaccagc
      601 agcacgaggt cgcggccgac catacgcgcc gccatcggat tctgccccgg cagctcgacc
      661 gtctgcagca cggcgccggt gcgcgcgtcc cagaacgcca gcacgcggtc actctcgctg
      721 gattccgtca ccagccgacc gtccggcgtg tacaggcaaa acccgaagtc gatccacaca
      781 tcggggccgc cgtcggacgc gacggtccac agcagcgagc gcgtctcgag gtcccagccc
      841 tgcagatgca tcacgctcga cacgtcgccg cggtccttct tgctggcacg tgcacggtcc
      901 gccgcgcgct cgaagtagcc ggtgctcgcc gcgtggcggc cgtccggcga gaccgccagg
      961 acgctgacct gctgggtctt gccggccagc gcgtgctcct ccccgccgtc ggcgaggtcg
     1021 tacagcgcga ggccctcgcc gaggaagccg accagcgcgc gctctcccgc cacgggcgcc
     1081 agcgcgtgcg ggttatgcga attttcggtc ggtgcacact gcactctgcg ccgcagcgct
     1141 ccggtctgcg cgtcccacac cgcgaagccg cgcgcctcgc cggtcagcgc cattccttcg
     1201 ccgagcccga gcgcgcaccc gttggcctcg tgctcgacat cgagggcgcg cagcggcccg
     1261 ggcacctcga gatcccagag caccacgccg ccgcccgtat cgacgctcag cgcccggtct
     1321 ccggccgtct ccagcttgtt gatcgcgtgc tggtgcggcc cgccaggccc cgcgcgcagg
     1381 ccctggtcga agtcccagac ctcgaccgcg ccctcgttcg tgcccgccaa caaccaccgc
     1441 ccgttcggcg agaagctcag cgcggtcacg tgttcaccgc cgcagccgag cgtggtgacc
     1501 agcgcgcccg tggcgagctc gcggacctgc atataaccat tgcccgccag taccagcagt
     1561 cgaccctccg ggtgcaccgc gaccgcctcg tgctcccagc cccagcgcgg ccagcggcgc
     1621 tgctcctcgc cggtcgccac gtcccagaag atcacgtcct tctcgatcga tgccagggtc
     1681 tcaccgccgt cgaggaacag cagccgccgc gccgcgtcgt ggtgcccctt cccggagtcg
     1741 ttgccggtcg cgatgtagag attaccgaag cccgggatcc gcaccacgtt ggccccggcg
     1801 tcgtagatcg tgcgcgccag ctcgccgctg gcgacgtccc acagcagcag ctgattatcg
     1861 aggcccgacg aggccgcgtg gcgcccgttc ggcgagatcg ccaccgtccg caggtgctcg
     1921 cggtgcgcct cgccgacctg cagcgcgcgg ccgctcgcga cgtcccagaa gcgcaccgtc
     1981 gcgtcctcgg cccccgagac caccaccgtg tcggtcgtcg ccacctcgcg cacgtacccg
     2041 ccgtgctcgt gcagcac
//
LOCUS       DASWNJ010000966         1927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_123173, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000966 DASWNJ010000000
VERSION     DASWNJ010000966.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123173"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..735)
                     /locus_tag="VGB85_16395"
     CDS             complement(94..735)
                     /locus_tag="VGB85_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135666.1"
                     /translation="MAEREEPGIETYRAPASSRLQALLHGGELAPVRPSFYMPGELRP
                     SQWVIVMASVAALLLMVRDAGWMIALALAVLVGSIHDDRKNVRLGVKILRRLRIEFEV
                     QLRSVTVPLGGWPFDGSDHTLSLAAIEVEVDHDPDQLERAVRLGEPRATLTFIADRVL
                     RVEFAWDRDPLGLERLDRLLFGVLLTHADELGVVRIRFRTPHESLTADQPAQK"
     gene            797..1447
                     /locus_tag="VGB85_16400"
     CDS             797..1447
                     /locus_tag="VGB85_16400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0135667.1"
                     /translation="MRGPDDVDDLELLRRWQAGQTSAGRELVSRHYAAVYRFFYGKVD
                     PGFCEDLTQATFEVLCRRRDAFRGEGASFRGFLFGIARMKLIEHIRGKLRAFDPMEHS
                     VVDPASAGSVSSLLAERQHEHLVAQALRELSLDDQVLLELKEYEQLTLRELAALFDVT
                     LGAIAGRVTRARERLRTATERLVADPGLRAASVHDLESCMRNIGTKIAAHLGGKDS"
     gene            1491..>1927
                     /locus_tag="VGB85_16405"
     CDS             1491..>1927
                     /locus_tag="VGB85_16405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135668.1"
                     /translation="MLPTLPPPPSPDAAALARGKARLLADISRLQGAEPTEDAAARSS
                     APPPAEPTQAPWIDEQAAWEASGADEPPASQRYRLAEVFARGGLGSVRRASDRKLGRT
                     VAIKELLRFDEAAVQRFAREAAITARLQHPGIIPLYDLGRDEHG"
BASE COUNT          275 a          696 c          699 g          257 t
ORIGIN      
        1 cggcggctgg gtcggcgtgt gggcggccaa gcgaatgccg caggcggtcg tgcgcgcgat
       61 cgtgtgcgcg gtcgggctgg tgctcgcggg ctactacttc tgggccggct gatcggcggt
      121 cagcgattcg tggggcgtcc ggaaccggat gcgcacgacg ccgagctcgt cggcgtgggt
      181 cagcaggacg ccgaacagca gacgatcgag gcgctcgagg ccgagggggt cgcggtccca
      241 cgcgaattcg acgcgcagga ccctgtcggc gatgaacgtg agcgtcgctc gcggctcacc
      301 gagccggacg gcgcgctcga gctggtccgg gtcgtggtcg acctcgacct cgatcgcggc
      361 gagagacagc gtatggtcgc tgccatcgaa gggccagccg ccgagaggca cggtgacgct
      421 gcggagctgg acctcgaatt cgatgcggag ccgccggagg atcttgacgc cgaggcgcac
      481 gttcttgcgg tcgtcgtgga tcgatccgac gaggaccgcc agggccagcg cgatcatcca
      541 gccggcgtca cggaccatga gcaggagcgc agcgacgctg gccatcacga tcacccactg
      601 cgacggccgc agctcgcccg gcatgtagaa gctcggccgc accggcgcga gctcccctcc
      661 gtgcagcagc gcctgcaagc gcgagctcgc cggggcgcgg taggtctcga tccccggctc
      721 ctcgcgctcg gccacgactc ggaggatagc gcgaacggtg cgtgacgccg ggccgcggct
      781 gggtcaccat cctcgggtgc gtggcccgga cgacgtcgac gacctcgagc tgctgcggcg
      841 ctggcaggcc ggacagacgt cggccgggcg cgagctggtc agtcgtcact acgcggcggt
      901 ctatcgcttc ttctacggca aggtcgaccc cggcttctgc gaggatctga cgcaggcgac
      961 cttcgaggtg ctgtgtcgcc ggcgcgacgc gtttcgcggc gagggtgcgt cgtttcgcgg
     1021 gttcctgttc ggcatcgccc ggatgaagct gatcgagcac attcgcggca agctgcgcgc
     1081 cttcgacccg atggagcaca gcgtcgtcga cccggcgagc gcgggctcgg tctcgagcct
     1141 gctggccgag cgccagcacg agcacctggt cgcccaggcg ctgcgcgagc tgagcctcga
     1201 cgaccaggtc ctgctcgagc tcaaggagta cgagcagctg acgctgcgcg agctggccgc
     1261 cctgttcgac gtgacgctcg gtgcgatcgc cggccgggtc acccgcgccc gcgagcgcct
     1321 gcggaccgcc accgagcgcc tggtcgccga ccccgggctg cgcgccgcca gcgtgcacga
     1381 cctcgagagc tgcatgcgca acatcggcac caagatcgcc gcccacctcg gcgggaaaga
     1441 ttcgtgaacg ggcaccgata aaactcgcgg ctcccctcac tcgagtgtcg atgctcccca
     1501 cgctcccgcc gccccccagt ccggatgccg ccgcgctcgc ccgcggcaag gctcgcctgc
     1561 tcgccgacat ctcgcgcctc cagggggccg agccgaccga ggacgccgcc gcacgctcgt
     1621 ccgcgccgcc gcccgccgag cccacgcagg ccccgtggat cgacgagcag gccgcgtggg
     1681 aggcctccgg agccgacgag ccgccggcga gccagcgcta ccgcctcgcc gaggtgttcg
     1741 cgcgcggcgg cctcggcagc gtgcgccgcg cgagcgaccg caagctcgga cgcacggtgg
     1801 cgatcaaaga gctgctgcgc ttcgacgagg cggcggtgca gcgcttcgcc cgcgaggccg
     1861 cgatcacggc gcgcctgcag caccctggca tcatcccgct ctacgacctc gggcgcgacg
     1921 agcacgg
//
LOCUS       DASWNJ010000967         4532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_614445, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000967 DASWNJ010000000
VERSION     DASWNJ010000967.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614445"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VGB85_16410"
     CDS             <1..735
                     /locus_tag="VGB85_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135669.1"
                     /translation="APAQPAAAVDTVTLRDGRLIRGTVAEVYPGRHVTIVSAAGERHT
                     IAWDQVADLNYGGQAVVPEPVAGPGRPHLHIETTRPAAVRLFEISTQMYAGGPRWGNY
                     PQQAQQVRPVCRAPCDRVIDGTPGQSFVFGGEGISPSRRFTLDEHEGPLLARVRPGRA
                     GVLVGGVLLTSLSIAPLITGTLFLSLSQHTAPDGSHGTRNAGITLSAVAFSMLVSGII
                     MIARGRTKVELYRRQAAQRRLNPARG"
     gene            complement(748..2121)
                     /locus_tag="VGB85_16415"
     CDS             complement(748..2121)
                     /locus_tag="VGB85_16415"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0135670.1"
                     /translation="MQAYGRCFIGVLLLAGCGPTGGSEETGGSSGDTTSGTTGPGPTN
                     TSSPDPTTSTTTPPTTSTSDTTEALTTTPDPTTSTTTDETTSTTDETTTSTTDDTTTD
                     TTTEDTTTGPMQMFTPLALEVADFDGDELADLLVLGVDNFDGVASRLSRGVGDGTFEV
                     GINPLLSGTSAFPVVGRLDPQPGADVMVAQDGGDVKVFRWAGDAFESWKVFTNANVPR
                     THVVHDVDGDDNEDIVWLWWTKNSLEFGLSIRPNAGNGFFAPVDTKIGGIEELGLAAT
                     SLLVGDFNGDDVADALVFEADQPKGMLRLFGTPAGFFGGPKFVLPTLKANVATVRDFD
                     EDGALDVLAFEQNPGRLVFCRGDGKGGFTIAETTEVVAPFVPSTFAIADIDADGHLDV
                     AVVDVASPELRIWGGLGDGTFAAPKSAALPSPAVRVHAAELDGDAGLELVAATFAAGD
                     VTVLLDP"
     gene            complement(2225..3079)
                     /locus_tag="VGB85_16420"
     CDS             complement(2225..3079)
                     /locus_tag="VGB85_16420"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0135671.1"
                     /translation="MPATFASLVGQSNFFITGCASGIGRHLADVLLAAGHQVFATDLD
                     EPALRAHAEASAWPIARAHTRRLDVTDAAAWEVVFAEAARVMGKVDVLINVAGYLLPG
                     YIADTDAAAVDRHLDVNTKGTIFGTQVAARHMLARRRGHIINIASLAALAPVPGIGLY
                     SASKYAVRGFSLAAAQELRPWGVAVTVVCPDAVQTPMLDLQVGYREAAMTFSGDRILT
                     VEDIADAVVQRVLPRRPLEVHLPRSRALLARLADLFPGTALWLRPWLEARGRARQAQI
                     QRNAARRA"
     gene            3161..3460
                     /locus_tag="VGB85_16425"
     CDS             3161..3460
                     /locus_tag="VGB85_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135672.1"
                     /translation="MKQFIWYLLFAVCVFVSYQGYVNSKDDPETEAMSRESVCALDKD
                     CGIAPAAQAGVRTTDIWSRRYQWSSKSGSYVATCKREYIFVGHWKCEAQPGQIGR"
     gene            complement(3561..>4532)
                     /locus_tag="VGB85_16430"
     CDS             complement(3561..>4532)
                     /locus_tag="VGB85_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0135673.1"
                     /translation="RDVLQNLKDGLAHLVFGQDEAVATVTAAIKRARAGLSRADKPIG
                     SFLFAGPTGVGKTELAKALAQLLAVPLIRFDMSEYMEKHSVSRLIGAPPGYVGFDQGG
                     LLTDAVAKSPHSVVILDEIEKAHPDLFSILLQVMDHATLTDTTGRKTDFRNVILIMTS
                     NAGAHEMTKRRMGFDEESDKVGDPKTALDRTFSPEFRNRLDRIVVFKSLPQDIILQVA
                     RKMLLELEAQLSDRGVRFTFSEAARAWIAKKGFDKLYGARPMARLIDEEIKGKLVDEL
                     LFGALEKGGHVAVDVAEDKLTFAFTALDAAEADAVSSVPEEVDESVN"
BASE COUNT          663 a         1631 c         1567 g          671 t
ORIGIN      
        1 gctcccgcgc agccggccgc ggccgtcgac accgtcacgc tacgtgacgg ccgcctcatt
       61 cgtggcacgg tggccgaggt gtatccgggt cgccacgtca cgatcgtgtc ggccgccggt
      121 gagcgccaca cgatcgcctg ggaccaggtc gccgacctca actacggcgg ccaggcggtc
      181 gtgccggagc cggtcgccgg ccccggccgc ccgcacctgc acatcgagac gacccgcccc
      241 gccgccgtgc gtctgttcga gatctcgacg cagatgtatg ccggcgggcc gcgctgggga
      301 aactacccgc agcaggccca gcaggtccgc ccggtgtgcc gcgcgccgtg tgaccgcgtc
      361 atcgacggca cccccggcca gtcgttcgtg ttcggcggcg agggcatcag cccgtcgcgg
      421 cgcttcaccc tcgacgagca cgagggcccg ttgctggccc gcgtgcggcc cggccgcgcc
      481 ggcgtactgg tgggcggcgt gctgctgacc tcgctgtcga tcgccccgct gatcaccggc
      541 accctgttcc tgtcgctgtc ccagcacacc gccccggacg gcagccacgg cacgcgcaac
      601 gccggcatca cgctctcggc ggtcgcgttt agcatgctgg tgtcgggcat catcatgatc
      661 gcccgcggcc gcaccaaggt cgagctgtac cgccgccagg ccgcgcagcg gcggttgaac
      721 ccggcccgcg gctgacagcg aacccgatca tgggtcgagc agcacggtga cgtcgcccgc
      781 ggcgaaggtc gcggcgacga gctccagccc cgcgtcaccg tcgagctcgg ccgcgtgcac
      841 gcgcaccgcc ggagacggca gcgcggccga cttcggcgcc gcgaaggtcc catcgccgag
      901 gccgccccag atccgcagct ccgggctggc gacgtcgacc acggcgacat cgaggtgccc
      961 gtccgcgtcg atgtcggcga tcgcaaaggt cgacggcacg aagggcgcca cgacctcggt
     1021 cgtctccgcg atcgtgaacc cacccttgcc gtcgccgcgg cagaacacca gccgcccggg
     1081 gttctgctcg aacgcgagca cgtcgagcgc gccgtcctcg tcgaagtcgc ggaccgtggc
     1141 gacgttggcc ttgagcgtcg gcagcacgaa cttcgggccg ccgaagaagc cggccggggt
     1201 gccgaacagg cgcagcatgc ccttgggctg gtcggcctcg aacaccagcg cgtcggcgac
     1261 atcatcgccg ttgaagtcgc cgacgagcag cgaggtcgcc gcgaggccga gctcctcgat
     1321 cccgccgatc ttggtgtcga ccggcgcgaa gaagccgttc ccggcgttgg gccggatcga
     1381 caggccgaac tcgaggctgt tcttggtcca ccacagccac acgatgtcct cgttgtcgtc
     1441 gccgtcgacg tcgtgaacga cgtgcgtgcg cggcacgttg gcgttggtga acaccttcca
     1501 cgactcgaag gcgtcgccgg cccagcggaa caccttcacg tcgccgccgt cctgcgcgac
     1561 catcacgtcg gcgcccggct gcgggtcgag ccgaccgacc accgggaacg cgctcgtacc
     1621 gctgagcagc gggttgatgc cgacctcgaa ggtcccgtcg ccgacgccgc gcgacagccg
     1681 actcgccaca ccatcaaagt tgtcgacgcc gagcaccagc aggtcggcga gctcgtcacc
     1741 gtcgaagtcc gcgacctcga gcgcgagcgg cgtgaacatc tgcatcggcc cggtcgtggt
     1801 gtcctcggtg gtcgtgtcgg tggtggtgtc gtcggtcgtg ctcgtcgtcg tctcgtcggt
     1861 cgtgctcgtc gtctcgtcgg tggtcgtcga ggtcgtcggg tcgggcgtcg tcgtcagcgc
     1921 ctcggtcgtg tcgctcgtcg aggtcgtcgg gggcgtggtg gtcgaggtcg tcgggtccgg
     1981 actggaggta ttggtcgggc ccggcccggt cgtgccgctg gtggtatcgc cgctcgaccc
     2041 cccggtctcc tcggacccgc ccgtcggccc acaccccgcg agcaacagca caccgatgaa
     2101 acaccgacca taagcttgca tctcgccagg gtggcacgcg ccgcggggcg cagcaagccg
     2161 gtcgactttt acctccagtc gaagcgtcgc tcgttgccgg tgcaacggcg cctgcggtcg
     2221 ccggctacgc gcgccgggcc gcgttgcgct ggatctgggc ctgacgggcc cggccgcggg
     2281 cctccagcca cgggcgcagc cacagggctg tgcccgggaa caggtcggcg aggcgggcca
     2341 ggagcgcgcg tgaccgcggc aggtggacct cgagcgggcg ccgcggcagc acgcgctgca
     2401 cgaccgcgtc cgcgatgtcc tcgaccgtca atatgcggtc gcccgagaag gtcatcgccg
     2461 cctcgcggta gccgacctgg aggtcgagca tcggcgtctg cacggcatcg ggacagacga
     2521 cggtgacggc gacgccccac gggcgcagct cctgggcggc cgccagcgag aagccgcgca
     2581 cggcgtactt cgacgccgaa tacaggccga tccccggcac cggcgcgagc gcggcgagcg
     2641 aggcgatgtt gatgatgtgt ccgcgccggc gcgcgagcat gtgccgcgcc gccacctgcg
     2701 tgccgaagat cgtgcccttg gtgttgacgt cgaggtggcg atcgacggcc gcggcgtcgg
     2761 tgtcggcgat ataaccaggc aggagatacc cggcgacgtt gatcagcacg tcgaccttgc
     2821 ccatcacccg cgcggcctcg gcgaacacca cctcccacgc ggccgcgtcg gtgacatcga
     2881 gccgccgcgt atgggcccgg gcgatcggcc aggcgctcgc ctcggcgtgc gcgcgcaggg
     2941 ccggttcatc gaggtcggtc gcgaagacct ggtgacccgc cgccaacaac acgtcggcca
     3001 ggtgccggcc gatgccgctg gcgcagcccg tgatgaagaa attcgactgg ccaacgagcg
     3061 aagcgaaagt ggcaggcacg gcgaacgagc ctaacacccg cgccactgtg gcgtgcgcgc
     3121 cggcttgccg cggcgcaggc gcccgtgcga ccatgccggc atgaagcagt tcatctggta
     3181 cctcctcttc gcggtgtgcg tgttcgtgag ctaccagggc tacgtcaact ccaaggacga
     3241 ccccgagacc gaggccatgt cgcgcgagtc cgtgtgcgcg ctcgacaagg actgcggcat
     3301 cgcccccgcc gcccaggccg gcgtccgcac caccgacatc tggtcccgcc gctaccagtg
     3361 gagcagcaag agcggcagct acgtggcgac ctgcaagcgc gagtacatct tcgtcggcca
     3421 ctggaagtgc gaggcacagc ccggccagat cgggcgctag tccgctcgcc gcgctcctgg
     3481 ccctcgcacc gcgcgaccgc cacgccaccc gcacccgcga gaccgccccg catacacggg
     3541 gcggtccacg cggacgaccg ctagttcacg ctctcatcga cctcttcggg gacggacgac
     3601 accgcgtccg cctccgcggc atcgagcgcc gtgaacgcga aggtcagctt gtcctccgcg
     3661 acatcgaccg cgacgtgccc gcccttctcg agcgcgccga acagcagctc atcgacgagc
     3721 ttgcctttga tctcctcgtc gatcaggcga gccatcggcc gcgcgccgta cagcttgtcg
     3781 aagcccttct tggcgatcca cgcgcgggcg gcctcgctga aggtgaagcg caccccgcgg
     3841 tccgagagct gggcctcgag ctcgagcagc atcttgcgcg cgacctgaag gatgatgtcc
     3901 tgcggcagcg acttgaacac gacgatgcgg tcgagccggt tgcggaattc cgggctaaac
     3961 gtgcggtcga gcgccgtctt cgggtcgccg accttgtcgc tctcctcatc gaaacccatg
     4021 cgccgcttgg tcatctcgtg cgcgccggcg ttgctggtca tgatgaggat gacgttacga
     4081 aagtcggtct tgcggccggt cgtatccgtc agcgtcgcgt ggtccatcac ctgcagcagg
     4141 atcgagaaca ggtccgggtg ggccttctcg atctcgtcga ggatgacgac cgagtgcggt
     4201 gacttggcga ccgcgtcggt cagcagtccg ccctgatcga agccgacgta gccgggcggc
     4261 gcgccgatca ggcggctgac gctgtgcttc tccatgtact cgctcatgtc gaagcggatc
     4321 agcggcaccg cgagcagctg ggcgagcgcc ttggcgagct cggtcttgcc gacgccggtc
     4381 ggcccggcga acaggaacga gccgatcggc ttgtccgcgc gcgacagacc ggcgcgcgcg
     4441 cgcttgatcg cggcggtgac ggtggcgacc gcctcgtcct ggccgaacac caggtgggcg
     4501 aggccatcct tgaggttttg caggacatcg cg
//
LOCUS       DASWNJ010000968         3581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_688442, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000968 DASWNJ010000000
VERSION     DASWNJ010000968.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688442"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="VGB85_16435"
     CDS             <1..1169
                     /locus_tag="VGB85_16435"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY0135674.1"
                     /translation="VLTLVTKPASKGKGKPTKRNRTPGPKIYARTGGGFTIRWKYRDA
                     NGVSQEIKVKAKGTTRTAAIAEARAIERSILDKMHADPAMRPAAPTVADFVPRFLDYC
                     RSNEKSPATIRNYGQHLRTYVIPVIGGMRLDAVTPSDYEAVKAACSHLSVATRRQVIA
                     SMARMMSVAMDLDAARNLPKPVQIKRPALNVKAYSPDEARALITACGEDTRDLAFVLL
                     GVHGGLRRGEVIAVRGEDFEEHPDGRLVLRVQRSVWNTTIKLPKSGRERVVALTREAS
                     AALRAHLVTLPDPRGYLFPAGVARLSDRGEPLPVEATAYVRAVQRLCKVAGIAYRATH
                     VLRKTAATAMAKGGASAWQIADMLGHAGIDMTKHYVDRAQAASTKAASLIEGYV"
     gene            complement(1234..2478)
                     /locus_tag="VGB85_16440"
     CDS             complement(1234..2478)
                     /locus_tag="VGB85_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEY0135675.1"
                     /translation="MPDPSTGDTAATSTSGTTGTTMGVDPGDSTTGTGGGTTDGPKLD
                     MGPQGCDPDLQATTFEFIWIANSTQGTVSKINTMTGIEVGRYRTAAEAANPSRTSVNQ
                     YGDVAVSNRHPGSITRINALASKCIDANNNGVIDTSTGPDDIRAWGTDECVVWHQSFP
                     SPDYNHGPRPTAWEGVIQDPVTCETPTPRLWGGWMDAVDTAHFVRLDGDTGMLLDEVL
                     RANWFGSGFGPYGGAVNSEGDLYAIGLETTVVHIDSETLVVTDIPPPPGLSSYGMAVD
                     KNGDIWVGSYGANSMYHYKVAEKQWYPLGPGGGWVLGLQADKKGRVWGAGTGPCRLVH
                     ADVATAKYVNTAIPLPGCSEPWGTSIDNEGFVWIVDKSNKAFKVNPDSYAVELVVTGL
                     VAPYTYSDQTEVVLKSLTVSLT"
     gene            complement(2885..>3581)
                     /locus_tag="VGB85_16445"
     CDS             complement(2885..>3581)
                     /locus_tag="VGB85_16445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135676.1"
                     /translation="CGFICDSTGEPGGSCDVRAQDCPAGEKCAPYSSDDDNTWDAAKC
                     VPVTGMGAPGDPCKNEGPYSGIDDCQAGALCWDPVAPDQAVCVALCQGSEAEPSCPAQ
                     LASHCVEFDAGTLELCLPICDPLAQDCANGHLCVYFPEDFVCMYDASGADAGAVFGPC
                     ERVNGCDIGLLCLEASAGVECDQNASGCCLPLCSLSDPAACPGAGQKCTPFYPEGMAP
                     AGHEDVGVCAVSP"
BASE COUNT          556 a         1231 c         1216 g          578 t
ORIGIN      
        1 ctgtcctcac tctcgttacg aagcccgcta gcaagggcaa gggcaagccg acgaagcgca
       61 accgtacccc cggcccgaag atctacgccc gcaccggggg cgggttcact atccgttgga
      121 agtaccgcga cgctaacggc gtgtcgcagg agatcaaggt caaggcaaag ggaacgacgc
      181 gcaccgctgc tatcgcggaa gctcgcgcca ttgagcggag catcctcgac aagatgcacg
      241 ccgatccggc gatgcgcccc gcggctccta ccgtcgctga cttcgtcccc cgcttcctgg
      301 actactgccg cagtaacgag aagtcgcccg cgacgatccg taactacgga caacacttgc
      361 gcacctacgt tatccccgtc atcggcggca tgcgcctgga cgcggtgacc ccgtccgatt
      421 acgaagccgt caaggccgcg tgctcgcacc tgtcagtcgc taccaggcgg caggttatcg
      481 cgtccatggc tcgcatgatg tccgtagcga tggacctgga cgcggcgcgc aacctgccta
      541 agccggtgca gatcaagcgg cccgcgctca acgtcaaggc gtacagcccc gacgaagccc
      601 gcgcgttgat caccgcttgc ggcgaggaca cccgcgacct ggcattcgtg ttgctcgggg
      661 tgcatggcgg gctgcgccgc ggtgaagtca tcgcggtacg cggcgaggat ttcgaggaac
      721 acccggacgg gcggctcgtg ctccgcgtcc agcggtccgt atggaacacc acgatcaagc
      781 tgccgaagtc tggacgcgag cgcgtggtag cgctgacgcg cgaagcgtcg gccgcgttgc
      841 gtgctcacct ggtaacgctc cccgatcccc gcggctacct gttccccgct ggtgtcgcgc
      901 ggcttagcga ccgcggcgag ccgcttcccg tggaagcgac ggcctacgtc cgcgcggtcc
      961 agcgcttgtg caaggtggcc gggatcgcct accgcgctac tcacgtgctg cgcaaaaccg
     1021 ccgcgaccgc catggccaag ggtggcgcta gcgcatggca gatcgctgac atgcttggcc
     1081 acgcggggat cgacatgacg aagcattacg tagaccgcgc gcaggcggcg agcaccaagg
     1141 ccgcgagtct catcgagggt tacgtctagc ggagcggtaa ggggtcgggc gtagataagg
     1201 gcccgttagg ggaaccgccc ccgaagcaag cgtctaagtt agcgagactg ttagggattt
     1261 tagaactacc tctgtttggt ccgagtaggt gtagggcgcg acgagtccgg tcaccaccag
     1321 ctcgacggcg tagctgtcgg ggttgacctt gaacgccttg ttggacttgt cgacgatcca
     1381 cacgaagccc tcgttgtcga tgctcgtgcc ccacggctcg ctgcagccgg gcagcgggat
     1441 cgcggtgttg acatacttgg ccgtcgcgac atccgcgtgc acgagccggc acggcccggt
     1501 gcccgcgccc cacacgcggc ccttcttgtc cgcttgcagc ccgagcaccc agccgccgcc
     1561 ggggccgagc gggtaccact gcttctcggc gaccttgtag tggtacatgc tgttggcgcc
     1621 gtagctgccg acccagatgt cgccgttctt gtcgaccgcc atgccgtagc tcgacaggcc
     1681 gggtggcggc gggatgtcgg tgacgaccag cgtctcgctg tcgatgtgca cgaccgtcgt
     1741 ctcgaggccg atcgcgtaga ggtcgccctc gctgttgacg gcgccgccgt acgggccaaa
     1801 gccgctgccg aaccagttcg cgcgcaggac ctcgtcgagc agcatgccgg tgtcgccgtc
     1861 gaggcggacg aagtgcgccg tgtcgacggc gtccatccag ccgccccaca gccgcggcgt
     1921 cggggtctcg caggtgacag ggtcctggat gacgccctcc cacgcggtcg gccgcgggcc
     1981 gtggttgtag tcgggcgagg ggaagctctg gtgccagacg acgcactcgt cggtgcccca
     2041 cgcgcggatg tcgtcgggcc cggtcgaggt gtcgatcacg ccgttgttgt tggcgtcgat
     2101 gcacttcgac gccagcgcgt tgatgcgggt gatgctgccg gggtggcggt tcgacaccgc
     2161 gacgtcgccg tactgattga ccgacgtgcg cgacgggttg gcggcctcgg ccgcggtccg
     2221 gtagcgaccg acctcgatgc ccgtcatcgt gttgatcttg ctgaccgtcc cctgggtgct
     2281 gttggcgatc cagatgaact cgaaggtcgt cgcctgcaga tcggggtcgc agccctgcgg
     2341 gcccatgtcg agcttcgggc cgtcggtcgt gccgccaccc gtgccggtcg tgctgtcgcc
     2401 cggatcgacg cccatcgtcg tgccggtcgt gcccgacgtg ctcgtcgcgg ccgtgtcgcc
     2461 ggtgctcggg tcgggcatcg tcgtggtgcc tgtccccccg gtggtcgtgc cgaccgtctc
     2521 gcctgccgtc gcccgagccc tcggtgacgc cagtggtgct cgtcgtcggc gtcgggcccg
     2581 gaccggtggc ccccgtcgag tcggtcatgc cgaccgtcgc agtcatcccc gtgctcgcgc
     2641 cctggctctc ggacgcggtc ggtgtgccgc cggcgtcgcc acatgcgggg accaccgcga
     2701 tcagaaggag gggaagtgcc tgtgcgccgg ggcgaagacg ggaccccatc cccgacagca
     2761 gaccagcgcg gcccgcagac cgcaagtgtc acgaacgagc gatccgttca tttgcgcgca
     2821 gactatccag gccgacccgg gtgccgtggg gcatacggat gtcggccgtc accgccgggt
     2881 cgcgtcacgg cgataccgcg catacaccga catcctcatg gcccgcgggg gccatgccct
     2941 cggggtaaaa cggcgtgcac ttctggccag cgcccggaca cgccgcgggg tccgacagcg
     3001 agcacagcgg caggcagcag ccggacgcgt tctgatcgca ctccacgccg gccgacgctt
     3061 cgaggcacag gagaccgatg tcgcagccgt tgacgcgctc gcaggggccg aagaccgcgc
     3121 cggcgtcggc ccccgaggcg tcgtacatac atacgaaatc ctcggggaag taaacgcaca
     3181 gatggccgtt cgcgcagtcc tgggccagtg gatcgcagat cggcaggcac agctcgagcg
     3241 tgcccgcgtc gaactccaca caatggctcg cgagctgggc cgggcagctc ggctcggctt
     3301 cactgccctg gcacagcgcg acacaaacgg cctgatccgg ggcgaccgga tcccagcaca
     3361 acgcgcccgc ctggcaatcg tcgatgccgg agtaggggcc ctcattcttg cacgggtcgc
     3421 ccggcgcgcc catgccggtc acgggcacgc acttcgcagc gtcccacgta ttgtcgtcgt
     3481 cggacgagta cggcgcgcac ttctcacctg cggggcagtc ctgggcgcgg acgtcgcagc
     3541 tcccgccggg ctccccggtc gagtcacaga taaagccaca a
//
LOCUS       DASWNJ010000969         2147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_229518, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000969 DASWNJ010000000
VERSION     DASWNJ010000969.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229518"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2147
                     /locus_tag="VGB85_16450"
     CDS             <1..>2147
                     /locus_tag="VGB85_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY0135677.1"
                     /translation="LDRDMQETWAIHGVYPALAWTPDNRELVFWAAGKLWRTLADGTQ
                     ATPTEIPFHVKTTRKVAPAVRFPIAVAPREFDVKALRDVAVSPDGTSVVYQALGHLYL
                     RTLPEGQPRRLTKPDDRFEQDPAFSRDGKSVVFTTWDDQALGSVRVVAVKGGKERVLT
                     ERPGHYVEPQFSPDGKRVVYRRLGGGWTRTPTWSQEQGIYVASTAARKGEAPALVTRD
                     GEEPHFGADPERVYYTAYGEDDKIQLESVMLDGSDHRKLIKTDGAMRFRVSPDGRWLA
                     WVEGFHALVAPMPEVGRTVELGPKSESLPVSRVTRDAGEQVQWSGDSQRLHWSLGPEL
                     YTREIKEAFSFVAGAPAKLPEAPEKGLNIAFKATQAAPKGTRAIVGGRVITMRGDEVI
                     DDATIVITGDRITAIGATGKVQVPAGAEVVRAEGKTIIPGLIDVHAHGSQGEDGITPE
                     QNWLHYATLTYGVTTIHDPSNDTHAIFAASELQRSGAIVGPRIFSTGTILYGAQAPFK
                     AEINSLDDARSHLRRLKAIGAFSVKSYNQPRREQRQQVLAAARELGMMVVPEGGSLFH
                     HNMTMVVDGHTGVEHAIPVANAYADVTQLWGHTRVGYTPTLGVAYGGLGGENYWYAQT
                     KVYDDTRLRRFVPAFAFEPRARRRTMAADNDWNHIRVAALAKKLLDAGVQVQMGAHGQ
                     REGLAAHWELWSLAQGGMKPIEVLRAGTIAGAQ"
BASE COUNT          349 a          779 c          751 g          268 t
ORIGIN      
        1 ctcgaccgcg acatgcagga gacctgggcg atccacgggg tctaccccgc gctcgcctgg
       61 acgcccgaca accgcgagct ggtgttctgg gccgccggca agctgtggcg caccctcgcc
      121 gacggcacgc aggcgacgcc gaccgagatc ccgttccacg tcaagacgac ccgcaaggtc
      181 gcgcccgcgg tgcgcttccc gatcgcggtc gccccgcgcg agttcgacgt caaggcgctg
      241 cgcgacgtcg ccgtcagccc ggacggcacg tcggtcgtgt atcaggcgct cggccacctg
      301 taccttcgga cattgccgga aggccagccg cggcgcctga cgaagccgga cgaccgcttc
      361 gagcaggacc ccgcgttctc gcgcgacggc aagagcgtcg tgttcacgac gtgggacgat
      421 caggcgctcg gcagcgtgcg cgtggtggcg gtcaagggcg gcaaggagcg cgtgctgacc
      481 gagcgcccgg gtcactacgt cgagccgcag tttagccccg acggcaagcg cgtcgtgtat
      541 cgccggctcg gcggcggctg gacgcgcacg ccgacctggt cgcaggagca gggcatctat
      601 gtcgcctcga cggccgcgcg caagggcgag gcgccggcgc tcgtcacccg cgacggcgag
      661 gagccgcact tcggcgccga ccccgagcgc gtgtactaca cggcctacgg cgaggacgac
      721 aagatccagc tcgagagcgt gatgctcgac ggcagcgatc accgcaagct gatcaagacc
      781 gacggcgcga tgcgctttcg cgtgtccccc gacggccgct ggctcgcgtg ggtcgagggc
      841 ttccacgcgc tcgtcgcacc gatgcccgag gtcggtcgga ccgtcgagct cgggcccaag
      901 tccgagagcc tgccggtgtc tcgcgtcacc cgcgacgccg gcgagcaggt ccagtggtcg
      961 ggcgacagcc agcgcctgca ctggtcgctc ggccccgagc tgtacacgcg cgagatcaag
     1021 gaggcgtttt cgttcgtcgc cggtgcgccg gccaagctgc ccgaggcgcc cgagaagggt
     1081 ctcaacatcg ccttcaaggc gacgcaggcg gccccgaagg gcacgcgggc gatcgtcggc
     1141 ggccgcgtga tcacgatgcg cggcgacgag gtgatcgacg acgcgacgat cgtgatcacc
     1201 ggcgaccgca tcaccgcgat cggggcgacc ggcaaggttc aggtcccggc cggcgccgag
     1261 gtcgtacgcg ccgagggcaa gacgatcatc cccggcctga tcgacgtgca cgcccacggc
     1321 tcacagggcg aggacggcat cacccccgag cagaactggc tgcactacgc gacgctgacc
     1381 tacggcgtga cgacgatcca cgacccgagc aacgacaccc acgcgatctt cgccgcgagc
     1441 gagctgcagc gcagcggggc gatcgtcggc ccgcggatct tctcgaccgg cacgatcctg
     1501 tacggcgcgc aggccccgtt caaggccgag atcaacagcc tcgacgacgc caggagccac
     1561 ctgcgccggc tcaaggcgat cggcgcgttc tcggtcaaga gctacaacca gccgcgtcgc
     1621 gagcagcgcc agcaggtgct cgccgcggcg cgcgagctcg gcatgatggt cgtgcccgag
     1681 ggcggctcgc tgtttcacca caacatgacg atggtcgtcg acggccacac cggggtcgag
     1741 cacgcgatcc cggtcgccaa cgcctacgcc gacgtcacgc agctgtgggg ccacaccagg
     1801 gtcggctaca cgccgaccct cggcgtcgcc tacggcggcc tcggcggcga gaactactgg
     1861 tacgcgcaga ccaaggtgta cgacgacacc cgcctgcgcc gcttcgtccc ggccttcgcc
     1921 ttcgagccgc gcgcccgccg gcgcacgatg gccgccgaca acgactggaa ccacatccgc
     1981 gtcgccgcgc tcgccaagaa gctgctcgac gccggcgtcc aggtgcagat gggcgcccac
     2041 ggccagcgcg agggcctcgc ggcccactgg gaattgtgga gcctggcgca gggcggcatg
     2101 aagccgatcg aggtgctgcg cgcgggcacg atcgccggcg cgcagta
//
LOCUS       DASWNJ010000970         2857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_195254, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000970 DASWNJ010000000
VERSION     DASWNJ010000970.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195254"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VGB85_16455"
     CDS             complement(<1..272)
                     /locus_tag="VGB85_16455"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY0135678.1"
                     /translation="MSTHATHDSPQPRLVVVAGLVWLGPDRLLVQRRPANAKHGAGML
                     ELPGGKVEPGEAPRAALARELTEEWGPHGGLLTLSLVAEVLHHVYP"
     gene            348..860
                     /locus_tag="VGB85_16460"
     CDS             348..860
                     /locus_tag="VGB85_16460"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HEY0135679.1"
                     /translation="MRLFPAKITTIVEAVNKTLVDSGDLEVSDRDEFKRDVESILKEY
                     LRKDREVTNRAKDELERRGLPYSDLFKTKRTMAEEQDFGIGEESVNWICSQLIELFMR
                     SSFVSEIYAEDSVIRKKLKEILRRHMQADDDIDREVRRHLKHLSENTSTFEIEYQRQL
                     ELIKRKHGLE"
     gene            860..1672
                     /locus_tag="VGB85_16465"
     CDS             860..1672
                     /locus_tag="VGB85_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135680.1"
                     /translation="MAHILVYAQRTPQGIHPASAAALCLARDIASDRGATITAVCPGD
                     AGRADQFVIGAVSRYGADLLTFCGPAGMRPLQERLRPLVCFVPHTEEGLAAAEGLAGG
                     PTETTWLHDPAIEWDNLAAVTSIVAGTLPWYDLPTIIDAEYDNEASLVDLPPWISRLD
                     AQFGRGPLRYFAPHDLDAKTRASLQSLGARQAPAEYLEQHDSGTLLWFSNATSGLPDL
                     LRGRGPGARVMYFPGADSQLHPSWTAADWVVPGEWPDAVKQLHSDLWKPALA"
     gene            1754..>2857
                     /locus_tag="VGB85_16470"
     CDS             1754..>2857
                     /locus_tag="VGB85_16470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135681.1"
                     /translation="MTAARALQALLAPDPALPVLIAEAAAEHVRLPGERLGEAVLARR
                     LRDEGAVVVVAHTIGPTLARALREQPPALLVLTGPADPDDPPAPPGTLLLGPDARLAL
                     ADDGELIVCASAAPELRTLLLALAASRLSPGTGTHGPQDMSPGPALPPGVRLAAAPTP
                     PWWPLWLADPRLARATALGFVAATSLHPGWARWAATTPDAAHVLVPLGLEPAKIDTPR
                     HPGLAAALAAHALERLTGPVFPSPEVLALLGRPQTPVPADSPDAAALARWAQARRALP
                     LFAAAPGMEQPLPGLSLASPTAAFIAQRVLLRRTQIRALLRTRGERPPVQLDPTAVAR
                     AEEVLRSAGQVLSEHESKVVLRGFGVEITRQAVA"
BASE COUNT          392 a         1131 c          968 g          366 t
ORIGIN      
        1 ctgggataga cgtggtgcag gacctcggcg accaaagaca gcgtgaggag tccgccgtgc
       61 gggccccact cctctgtgag ttcgcgggcc agcgccgccc gcggggcctc gccgggttcg
      121 accttgccgc cgggcagctc cagcatgccg gcgccgtgtt tggcgttcgc gggccggcgc
      181 tgcacgagca ggcgatcggg gccgagccag acgaggccgg cgacgacgac gagacgcggc
      241 tgaggggagt cgtgggtggc gtgcgtggac atgcgggggc cggtgtcggg agccttagcc
      301 caagctggac gctcggaaaa gcagcgccta tcctgcgggt cacccccatg cgcctttttc
      361 cagcgaagat caccaccatc gtcgaagccg tgaacaagac actggtcgac tccggggacc
      421 tcgaggtcag cgaccgtgac gagttcaaac gcgatgtgga gtcgatcctc aaggagtacc
      481 tccgcaagga ccgcgaggtg accaaccgcg ccaaggacga gctcgagcgg cgcggcctgc
      541 cctactccga cctgttcaag accaagcgca cgatggccga ggagcaagac ttcggcatcg
      601 gcgaggagtc ggtcaactgg atctgcagcc agctgatcga gctgttcatg cgctcgagct
      661 tcgtcagcga gatctacgcc gaggacagcg tgatccgcaa gaagctcaag gagatcctgc
      721 gccgccacat gcaagccgat gacgacatcg accgcgaggt tcgccggcac ctcaagcacc
      781 tcagcgagaa caccagcacc ttcgagatcg agtaccagcg gcagctcgag ctcatcaagc
      841 gcaagcacgg tctagagtaa tggctcacat cctggtctac gcccagcgga cccctcaggg
      901 gatccatcct gccagcgccg ccgcgctgtg cctggcccgt gacatcgcga gcgatcgcgg
      961 cgccacgatc acggccgtgt gcccgggcga cgccggtcgc gcggaccagt tcgtcatcgg
     1021 tgcggtcagc cgttacggcg ccgacctgct gaccttctgc ggccccgccg gcatgcgccc
     1081 gctgcaagag cggctgcgtc cgctggtctg cttcgtgccg cacaccgagg agggcctcgc
     1141 cgcggcggag ggcctcgccg gcggtccgac cgagaccacc tggctgcacg accccgcgat
     1201 cgagtgggac aacctcgcgg ccgtcacctc gatcgtcgcc ggcaccctgc cctggtacga
     1261 cctgccgacg atcatcgacg ccgagtacga caacgaggcc agcctggtcg acctgccgcc
     1321 gtggatctcg cgcctcgacg cgcagttcgg ccgcggcccc ctgcggtact tcgccccgca
     1381 cgacctcgac gccaagaccc gcgcctcgct gcagtcgctc ggcgcccgtc aggccccggc
     1441 ggagtacctc gagcagcacg acagcggcac cctgctgtgg tttagcaacg ccacgtccgg
     1501 gctccccgac ctgctgcgtg gccggggccc cggcgcccgc gtcatgtact tccccggcgc
     1561 cgactcccag ctccacccga gctggaccgc cgccgactgg gtcgtgcccg gcgagtggcc
     1621 cgacgccgtc aagcagctcc acagcgacct gtggaagccc gcgcttgcct gagcgccggc
     1681 gcctcggcag ggtgagcctc ggcagggtga gcctcggcag gatgagcaag gtgctgccag
     1741 gggaagggtg gtcgtgaccg ccgcgcgcgc cctgcaggcc ctgctcgcgc ccgacccggc
     1801 gctgcccgtg ctgatcgccg aggccgccgc cgaacacgtg cgcctgcccg gcgagcggct
     1861 cggcgaggcc gtgctcgccc gccgcctgcg tgacgagggc gccgtcgtcg tcgtcgccca
     1921 cacgatcggg cccaccctcg cccgcgcgct gcgcgagcag ccgcccgcgc tgctcgtgct
     1981 caccggaccc gccgacccgg acgacccgcc cgcgccgcct gggaccctgc tgctcggccc
     2041 cgacgcccgc ctggcgctcg ccgacgacgg cgagctgatc gtctgcgcca gcgcggcccc
     2101 cgaattgcgg acgctgctgc tcgcgctcgc ggcgagccgc ctgtcccccg ggacaggtac
     2161 acatggcccg caggacatgt ccccggggcc agccctaccc cccggcgtcc gcctggccgc
     2221 cgccccgacc ccgccgtggt ggccgctgtg gctcgccgac ccgcgcctcg cccgggcgac
     2281 cgcgctcggc ttcgtcgccg ccaccagcct gcaccccggc tgggcccgct gggccgcgac
     2341 cacgcccgac gccgcccacg tgctcgtgcc gctcggcctc gagcccgcga agatcgacac
     2401 gccccgccac cccggcctcg ccgccgccct cgccgcccac gcgctcgagc gcctgaccgg
     2461 cccggtgttc ccgagccccg aggtgctggc cctgctcggg cgcccgcaga cacctgtccc
     2521 tgcggacagc cccgacgcgg ccgcgctcgc gcgctgggcc caggcccgcc gcgcgctgcc
     2581 gctgttcgcc gccgccccgg gcatggagca gccgctgccc gggctgtcgc tcgcctcgcc
     2641 gaccgccgcg ttcatcgccc agcgcgtgct gctgcgccgc acgcagatcc gcgcgctgct
     2701 gcgcacccgc ggcgagcgcc cgccggtgca gctcgacccg accgcggtcg cgcgcgcgga
     2761 ggaggtcctg cgcagcgccg gccaggtgct ctcggagcac gagagcaagg tggtgctgcg
     2821 cggcttcggc gtcgagatca cgcgccaggc ggtcgcc
//
LOCUS       DASWNJ010000971         2727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_442664, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000971 DASWNJ010000000
VERSION     DASWNJ010000971.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442664"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VGB85_16475"
     CDS             complement(<1..717)
                     /locus_tag="VGB85_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY0135682.1"
                     /translation="MFDWVYGLFSNDLAIDLGTATTLTYVKGRGIVAHEPSVVAVQRK
                     GNTKRVVAVGKDAKEMLGRTPGNIVAIRPMKDGVIADFEVTEAMMRHFINRAHNRQTL
                     VKPRIVICVPSGITEVEKRAVRDSAMAAGAREVFLIEEPMAAAIGAGLPITEPGGNMV
                     VDIGGGTAEVAVISLAGIVACKSIRVGGDKMDEAIIRHVRNAYNLMIGDPTAEEIKIR
                     VGSAYPLAQELRMEIRGRDLV"
     gene            1047..>2727
                     /locus_tag="VGB85_16480"
     CDS             1047..>2727
                     /locus_tag="VGB85_16480"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEY0135683.1"
                     /translation="MLQALRSSLKTVIVWIFLGVLALGFGLSFGLPSDAITCGAEPIA
                     KAYGENILDEDYQYQFQAISLVLPIPKDAKFQETMGLKQEVLDAVVERRVLAKIGERM
                     GLAASKEDAEELTADGHVIVLGNTYDWLGDGAFNYEMFKQRHLPRFMITEPKYLEYQR
                     QELLARTVRDLIASTITISEAELRAEYEGRQNQLSLRYARFEAARFSDLVDPSEAEVS
                     KYVEAHKDELTKQFEAQGVRFTKLPKQVRLRFMQVKKPGPPAEDADKARKAEYDAALK
                     AARAKIDAAMARLTAGEDFRVVARSVSEDAMTAPRGGEYGWTSVEGTGSGLDPIIDTT
                     AAGLTAGKSSPVVESEEAFYVVRVEAVREGDVAQEDALRELAEEGVVRERGKALAKQA
                     AAEALQALKDGKKLTELFAAPEAEGIEGLAVLGEATPPKETSGTKPELRVTGLFARER
                     PIPGLGMNPELTRAAWAADPKAEFIDGTFETEDGVVIAAVERKETATDEGLAAARAEL
                     YRELAARKAGKVTARFAFNQCLADKARGEIVPHQAKIQRLMTYETKVAVDED"
BASE COUNT          445 a          851 c         1038 g          393 t
ORIGIN      
        1 aacgaggtcg cgcccgcgga tttccattcg aagctcctga gcgagggggt aggcggatcc
       61 gacgcggatc ttgatctcct ccgcagtggg gtcgccgatc atcaggttgt aggcgttgcg
      121 gacgtggcgg atgatcgcct cgtccatctt gtcgccgccg acgcggatcg acttgcaggc
      181 gacgatgccg gcgagggaga tcaccgcgac ctccgcggtg ccgccgccga tgtcgacgac
      241 catgttgccg ccgggctcgg tgatcggcag gccggcgccg atcgccgcgg ccatcggctc
      301 ctcgatgagg aagacctcac gggcgccggc ggccatcgcc gagtcacgca cggcccgctt
      361 ctcgacctcg gtgatgccgg acggcacgca gatgacgatc cgcggcttga cgagggtctg
      421 gcggttgtgg gcccggttga tgaagtgccg catcatcgcc tcggtgacct cgaagtcggc
      481 gatgacgccg tccttcatcg ggcggatggc gacgatgttt ccgggggtgc ggccgagcat
      541 ctccttggcg tccttaccga cggcgacgac gcgcttggtg ttgcccttac gctggacggc
      601 gacgacgctg ggctcgtgcg cgacgatccc gcggcccttg acgtaggtca gggtcgtcgc
      661 ggtcccgaga tcgatggcca gatcgttcga aaacaggccg tatacccagt caaacagcat
      721 ttagtggcga tctgggccag gtgggccgaa ggcggagcct gagggtggga tccttgggag
      781 gaacggaggc cggaggaagg ggggccgggc ctgctcccat atacccgcgt gggggtctgg
      841 gcaagctagt gcaccgacgg ggagggggcc gggcggggga atttgaggta attgcgctct
      901 ttctcacgag ataggggcgt tttgcgcccg gccgcacagt gtgggcgtgc gcggggcggg
      961 ctgcaactct cgacgcccca cgggtattcc gggtcgttct acgggctttc acatcgagca
     1021 ccgggggcgc taccttcccg gtgctcatgc ttcaagccct gcgttcctcg ctcaagaccg
     1081 tgatcgtctg gatcttcctc ggcgtgctgg cgctcggctt cggcctcagc ttcggcctgc
     1141 cgagcgacgc gatcacctgc ggcgccgagc cgatcgccaa ggcctacggc gaaaacatcc
     1201 tcgatgagga ctatcagtac cagttccagg ccatctcgct ggtgctgccg atcccgaagg
     1261 acgcgaagtt ccaggagacg atggggctca agcaggaggt gctcgacgcg gtggtcgagc
     1321 gccgcgtgct cgccaagatc ggcgagcgca tgggcctggc cgcgtccaag gaggacgccg
     1381 aggagctgac ggccgacggc cacgtgatcg tgctcggcaa cacctacgac tggctcggcg
     1441 acggcgcctt caactacgag atgttcaagc agcgccacct gccgcgcttc atgatcaccg
     1501 agccgaagta cctcgagtac cagcgccagg agctgctcgc gcgcacggtc cgcgacctga
     1561 tcgccagcac gatcacgatc tccgaggccg agctgcgcgc cgagtacgag gggcgccaga
     1621 accagctgtc gctgcgctac gcccgcttcg aggcggcccg cttctcggac ctcgtcgacc
     1681 cgtcggaggc cgaggtcagc aagtacgtcg aggcgcacaa ggacgagctg acgaagcagt
     1741 tcgaggcgca gggggtgcgc ttcaccaagc tgccgaagca ggtgcgcctg cgcttcatgc
     1801 aggtcaaaaa gccgggcccg ccggcggagg acgccgacaa ggcgcgcaag gccgagtacg
     1861 acgcggcgct caaggcggcg cgcgcgaaga tcgacgcggc gatggcgagg ctgacggcgg
     1921 gcgaggactt ccgggtggtc gcgcgctcgg tgtcggagga cgcgatgacg gccccgcgcg
     1981 gcggtgagta cggatggacg agcgtcgagg gcacgggctc gggcctcgac ccgatcatcg
     2041 acacgacggc ggcggggctg acggccggca agagctcgcc ggtggtcgag agcgaggagg
     2101 cgttctacgt cgtgcgcgtc gaggcggtgc gcgagggcga cgtggcgcag gaggacgcgc
     2161 tgcgcgagct ggcggaggag ggcgtggtgc gcgagcgcgg caaggcgctg gcgaagcagg
     2221 ccgccgcgga ggcgttgcag gcgctcaagg acggcaagaa gctgacggag ctgttcgcgg
     2281 ccccggaggc ggagggcatc gaggggctgg cggtgcttgg cgaggcgacg ccgccgaagg
     2341 agacctcggg gaccaagccg gagctgcgcg tgacgggcct gttcgcgcgc gagcggccga
     2401 tccccgggct cggcatgaac ccggagctga ccagggcggc gtgggcggcg gacccgaagg
     2461 cggagttcat cgacggcacc ttcgagacgg aggacggcgt cgtgatcgcg gcggtcgagc
     2521 gcaaggagac cgcgaccgac gagggcctgg cggccgcgcg cgcggagctg taccgcgagc
     2581 tggcggcgcg caaggccggc aaggtgacgg cgcgcttcgc cttcaaccag tgcctggccg
     2641 acaaggcccg cggcgagatc gtgccgcatc aggcgaagat ccagcggctg atgacctacg
     2701 agaccaaggt cgcggtcgac gaggacg
//
LOCUS       DASWNJ010000972         2466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_87718, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000972 DASWNJ010000000
VERSION     DASWNJ010000972.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87718"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VGB85_16485"
     CDS             <1..254
                     /locus_tag="VGB85_16485"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY0135684.1"
                     /translation="HMVPGSVTVKAGDKVELGQTLGKVGNSGRSTGPHIHFQVSSDPF
                     PESGKQIGLQAPFVDVYVDGLWYPRKMPVRGDRVRRSKL"
     gene            complement(251..1210)
                     /locus_tag="VGB85_16490"
     CDS             complement(251..1210)
                     /locus_tag="VGB85_16490"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEY0135685.1"
                     /translation="MRAAMDSFLVHLESERRASPHTLRAYRRDLEDFLARVEARRGRA
                     AVPADLNLREIRSDLADLYGAVQASTMARKLSALRSFGEFMRQRHLVADNEAALVRRP
                     RQRVRLPVALPVEDIGPMIDGSGHADGPTGLRDRAILEVLYGAGLRVSECAALDLGHL
                     VWSGDDLSLRVVGGKGGKDRIVPLGSAGATAMRAWLAVRDALAPAELSPETGRKGQAR
                     VAAPVFLSKKGQRMSTRTIRALVYRRCEATGTRARVGPHGLRHSFATHLLESGCDLRS
                     IQSMLGHASLSTTQRYTHLDIGHLTSIYEKAHPRATHPPRDDR"
     gene            complement(1257..>2466)
                     /locus_tag="VGB85_16495"
     CDS             complement(1257..>2466)
                     /locus_tag="VGB85_16495"
                     /inference="COORDINATES: protein motif:HMM:NF025224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEY0135686.1"
                     /translation="VLRRLVALRHLDVGRGVVTDLGPLAELTSLESLVLSDNQITDIS
                     ALAGLDALFALELDGNRVTDITPLTGLVGLEYLDLDKNQLTSLAPLAGLTRLRDLEAS
                     DNMLADTAGLADKPNLVELSLEVNQIAELGDLRGLDSLVTLDLDGNALKNLEGLAVVT
                     RLRSFSARSNALTDIGGLATLTGLRSLDLRDNPGLVSLAPLASLVALDFLGAGGHGLP
                     QDLAPLTGMPLLRNVILAEAGTADYTPLTSLPALRVLDLGGATVDDADLPAIGQIVGL
                     TDLSVRGTAITDLGPLAPLTGLVSFDASDTAVASVAAVSAWTDIEELDLAGTKIPDLA
                     GFNLLKYLRGVDISDTPVSSLAPLVANEYFHRGDHVDATGAALGAEDCADILALRARE
                     AIVDTAVACE"
BASE COUNT          338 a          759 c         1026 g          343 t
ORIGIN      
        1 ggcacatggt gcccggcagc gtgacggtca aggcgggcga caaggtcgag ctcgggcaga
       61 cgctgggcaa ggtcggcaac tcggggcgca gcacggggcc gcacatccac ttccaggtct
      121 cgagcgaccc gttcccggag agcggcaagc agatcgggct gcaggcgccg ttcgtcgacg
      181 tttacgtcga tgggctgtgg tacccgcgca agatgccggt gcgcggcgac cgggtgcgtc
      241 gcagcaagct ctagcgatcg tcgcgcggcg ggtgggtcgc gcgcgggtgg gccttctcgt
      301 agatgctcgt caggtggccg atgtcgaggt gggtgtagcg ctgggtcgtc gacaggctgg
      361 cgtggccgag catgctctgg atgctccgca ggtcgcagcc cgactcgagc aggtgggtgg
      421 cgaagctgtg gcgcaggccg tgcgggccga cgcgcgcgcg ggtgccggtc gcctcgcagc
      481 ggcgatacac gagcgcgcgg atcgttcgcg tcgacatgcg ctggcccttc ttcgacagga
      541 acacgggggc ggcgacgcgg gcctggccct tgcgacctgt ctccggggac agctcggcgg
      601 gcgcgagggc gtcgcgcacg gcgagccagg cgcgcatggc ggtcgctccg gcgctgccga
      661 gcgggacgat gcgatccttg ccgcccttgc cgccgacgac gcgcaggctg aggtcgtcgc
      721 cggaccacac gaggtgaccg aggtcgagcg cggcgcactc gctgacgcgc aggccggcgc
      781 cgtagaggac ctcgaggatc gcgcggtcac gcaggccggt ggggccgtcg gcgtggccgg
      841 agccgtcgat catcgggccg atgtcctcga ccggcaaggc gaccggcagg cgcacgcgct
      901 gcctgggacg gcggaccagc gcggcctcgt tgtcggcgac gagatggcgc tggcgcatga
      961 actcgccgaa gctgcgcagc gccgagagct tgcgcgccat ggtgctcgcc tggacggcgc
     1021 cgtagaggtc ggcgaggtcg ctgcgaattt cgcggaggtt gaggtcggcc gggaccgcag
     1081 cgcgcccgcg gcgggcctcg acgcgggcca ggaagtcctc gaggtcgcgc cggtaggccc
     1141 gcagggtgtg cggcgaggcc cgtcgctcgc tctcgaggtg gacgaggaag ctgtccatgg
     1201 cggcgcgcat gacctccggt gtagtgccgg cggggcttgc gagcaagaac caggctttac
     1261 tcgcaggcga ccgcggtgtc gacgatcgct tcgcgcgcgc gcagggcgag gatgtcggcg
     1321 cagtcctcgg cgccgagcgc cgcgccggtg gcgtcgacgt ggtcgccgcg gtgaaagtac
     1381 tcgttggcga ccagcggcgc gagcgagctt accggggtgt cgctgatgtc gacgccgcgc
     1441 aggtacttga gcaggttgaa gccggcgagg tcggggatct tggtgccggc gaggtcgagc
     1501 tcctcgatgt cggtccaggc ggacaccgcg gcgaccgagg cgacggcggt gtcgctggcg
     1561 tcgaacgaca cgaggccggt cagcggagcg agcggcccga ggtccgtgat cgcggtcccg
     1621 cggacgctga ggtcggtgag cccgacgatc tgaccgatcg cgggcaggtc ggcgtcgtcg
     1681 acggtcgcgc cgccgaggtc gaggacgcga agcgccggca agctggtcag cggcgtgtag
     1741 tcggcggtgc cggcctcggc gaggatcacg ttgcgcagca gcggcatgcc ggtcagcggg
     1801 gcgaggtctt gcggcagccc gtggccgccg gcgccgagga agtcgagggc gaccagcgac
     1861 gcgagcggcg cgagcgagac gagcccgggg ttgtcgcgca ggtcgagcga gcgcaagccg
     1921 gtcagcgtgg cgaggccgcc gatgtcggtc agggcgttgc tgcgggcgct gaagctgcgc
     1981 aagcgcgtga cgacggcgag gccctcgagg ttcttcaagg cgttgccgtc gaggtcgagg
     2041 gtgacgagcg agtcgagccc gcgcaggtcg ccgagctcgg cgatctggtt gacctcgagc
     2101 gagagctcga ccaggttcgg tttgtcggcg aggccggcgg tgtcggcgag catgttgtcg
     2161 ctcgcctcga ggtcgcgcag gcgcgtgagg ccggcgagcg gggcgaggct ggtcaattga
     2221 ttcttgtcga ggtcgaggta ctcgaggccg acgaggccgg tgagcggcgt gatgtcggtg
     2281 acgcggttgc cgtcgagctc gagcgcgaac agggcgtcga ggccggcgag cgcgctgatg
     2341 tcggtgatct ggttgtccga gagcacgagc gactcgaggc tggtcagctc cgccagcggg
     2401 ccgaggtcgg tgacgacgcc gcggccgacg tcgaggtggc gcagggcgac gagcctgcgc
     2461 agcacg
//
LOCUS       DASWNJ010000973         2761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_442674, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000973 DASWNJ010000000
VERSION     DASWNJ010000973.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442674"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1538)
                     /locus_tag="VGB85_16500"
     CDS             complement(<1..1538)
                     /locus_tag="VGB85_16500"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY0135687.1"
                     /translation="MAESPRSTPDPLPGVPPDRPGTPGDGSVDKIRPVERLRRVFSYT
                     RPHRWRLVAALACLFIASGLGLIHPYFFGELAGAAFTSGSAIDKERAYHALGENSIAL
                     VAIFMAQAVFIFFRHYYMTWLGERVVTDLRIDLYRHLATMPQSFFHRTRTGELLSRLS
                     DDVTRLQSTVGQDLSIFLRNALQLIGGVVILFYMNWLLTAAMLAVVPALMIAANYWSR
                     IIREISREAQDELARASGGLQEGLSAIETVQAFTRERFEVDRYAGALERTFGLFIRRT
                     LARSWFASVISFFAFTSIAGVFWLGGSMVIEGTISASNLMSFLLYTLIVAGAVGSLAE
                     LFGGLQSTFGATARIFEILDTTPGIADPPNPVENPQLGGALDFAGVSFTYADRDTPVV
                     QDVNLHVEPGEVCALVGPSGSGKTTLGRLVLRFWDPSTGVIKLDGHDLRSLRLADLRG
                     VMAVVSQDPVLFSGTIRENIRYGRLDATDAEIEAAARAANADGFIREFPDGYATIVGE
                     RGVKL"
     gene            complement(1560..2501)
                     /locus_tag="VGB85_16505"
     CDS             complement(1560..2501)
                     /locus_tag="VGB85_16505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HEY0135688.1"
                     /translation="MQTRLLTPLLVSLLLAAPLACGDDKGGAETETATGTSTSTTGET
                     STGETPTTTGEDPSTSTTSTSGETPTTTDPTTTGAGDQDMDGVIDGVDNCPSDANPNQ
                     LDFDGNAVGNVCDEPLSFTIADGVPPEFNKLDTTATANKTLECMFPVSLVVLNGEVQV
                     TLDDAGIARVYAAKLNFADTPELTCDLTLVKVKLKITEFFADGPDPFLVGFPFTLPDH
                     DNGTITGNTDMPHTILVSGLINVTESSNEDLAPTGESALEMVPGAFPNALASVVNKGE
                     QVSMLFTDSNSVLFEQTTMSGIKITLTGLNGTLRLKR"
BASE COUNT          449 a          901 c          974 g          437 t
ORIGIN      
        1 agcttgaccc cgcgctcgcc gacgatcgtc gcgtagccgt ccgggaattc gcggataaag
       61 ccgtccgcgt tggcggcccg ggcggccgcc tcgatctccg cgtcggtcgc gtccaggcgg
      121 ccgtagcgga tgttctcgcg gatcgtgccg ctaaacagca ccgggtcctg cgagacgacg
      181 gccatcacgc cgcgcaggtc ggcgaggcgc aggctgcgaa ggtcgtggcc gtcgagcttg
      241 atcacgccgg tcgacggatc ccaaaagcgc agcaccagcc ggccgagcgt cgtcttgccc
      301 gagccgctgg gaccgacgag cgcacagacc tcgccgggct cgacgtggag attgacgtcc
      361 tggacgaccg gcgtgtcgcg gtcggcatat gtaaaggaca ccccggcgaa gtcgagcgcg
      421 ccgccgagct gcgggttctc gaccgggttc ggcggatcgg cgatgcccgg ggtcgtgtcg
      481 aggatctcga agatgcgggc cgtcgcgccg aaggtgctct gcaacccgcc gaacagctcg
      541 gcgagcgatc cgaccgcgcc ggcgacgatc aatgtataga gcaggaagct catcagatta
      601 ctggcggaga tcgtcccctc gatcaccatg ctgccgccga gccagaacac ccccgcgatc
      661 gacgtgaacg cgaagaacga gatcacgctg gcgaaccagc ttcgcgccag cgtgcggcgg
      721 atgaacaggc cgaaggtgcg ctcgagcgcg ccggcgtagc ggtcgacctc gaagcgctcg
      781 cgcgtgaacg cctgcaccgt ctcgatcgcc gacaggccct cctgcaggcc gccggacgcg
      841 cgcgcgagct cgtcctgggc ctcgcggctg atctcgcgga tgatgcggct ccagtagttc
      901 gccgcgatca tcagcgctgg cacgaccgcg agcatcgccg cggtcaacag ccagttcata
      961 tagaacagga tcaccacgcc gccgatcagc tgcaatgcat tgcgcaggaa tatgctcagg
     1021 tcctggccga ccgtgctctg taagcgcgtg acgtcgtccg acaggcgcga gagcagctcg
     1081 ccggtgcgcg tgcgatgaaa gaagctctgc ggcatcgtcg ccaggtggcg ataaagatcg
     1141 atgcgcaggt cggtgacgac ccgctcgccg agccaggtca tgtaatagtg gcgaaagaag
     1201 atgaagaccg cctgcgccat gaagatcgcg accagcgcga tcgagttctc gccgagcgcg
     1261 tggtaggccc gctccttgtc gatcgcggag ccgctggtga acgcggcccc ggcgagctcg
     1321 ccgaagaagt acgggtggat caggcccagg ccggaggcga tgaacaggca cgcgagcgcg
     1381 gcgacgaggc gccagcggtg cggccgcgtg tacgagaaga cccgccgcag tcgctcgacg
     1441 ggtcggatct tgtcgacgga cccgtcaccc ggggtgccgg ggcgatcagg cgggacgccg
     1501 gggagcggat cgggcgtgct gcgcggagac tcggccatgg agccgcgcag ggtagcacct
     1561 caccgcttca ggcgcagggt gccgttgagg ccggtcagcg tgatcttgat gcccgacatc
     1621 gtcgtctgct cgaacaggac cgagttgctg tcggtgaaca gcatcgagac ctgctcgccc
     1681 ttgttgacga ccgaggccag cgcgttcggg aaggcgcccg ggaccatctc gagcgcgctc
     1741 tcgccggtcg gcgcgaggtc ctcgttgctc gactcggtga cgttgatcag gccgctgacg
     1801 aggatcgtgt gcggcatgtc ggtgttgccg gtgatcgtgc cgttgtcgtg gtcgggcagc
     1861 gtgaacggga agccgaccag gaagggatcg gggccgtcgg cgaagaactc cgtgatcttg
     1921 agcttgacct tgaccagcgt caggtcgcac gtcagctccg gcgtgtcggc gaagttgagc
     1981 ttggccgcgt agacccgggc gatgcccgcg tcgtcgaggg tcacctgcac ctcgccgttt
     2041 agcacgacga ggctgaccgg gaacatgcac tcgagcgtct tgttggcggt cgccgtggtg
     2101 tcgagcttgt tgaactcggg cggcacgccg tcggcgatcg tgaagctcag cggctcgtcg
     2161 cacacgttgc cgaccgcgtt gccgtcgaag tcgagctggt tcgggttcgc gtcggagggg
     2221 cagttgtcga cgccgtcgat cacgccgtcc atgtcctggt cgcccgcgcc cgtcgtcgtc
     2281 gggtccgtcg tcgtcggcgt ctcaccgctc gtcgaagtcg tcgaagtcga agggtcctcg
     2341 ccggtcgtcg tcggcgtctc gccggtcgac gtctcaccgg tcgtgctcgt gctcgtgccg
     2401 gtcgcggtct cggtctcggc gccgcccttg tcgtcaccac aggcgagcgg cgcggcgagc
     2461 aggaggctga cgagcaatgg cgtaaggagg cgggtctgca tcatgcccgc atatcacgaa
     2521 aagcgcgccg cgcggcgacg taccgcgtcg gctcacgctg cgagcggcac accgcgggtg
     2581 caggcaccat cggcctgcgc agacgcgcag ccgcccctgc accccggagt cggtcacggg
     2641 tgacgagctg gccaggtggc ttgtcgagcg ttgccagtgc aactcgagtt gccagcaccg
     2701 ccgcttttgc cacccccgaa gctcgccaga aaaccctgtg atctcaacac taccagtcga
     2761 c
//
LOCUS       DASWNJ010000974         2231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_123239, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000974 DASWNJ010000000
VERSION     DASWNJ010000974.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123239"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VGB85_16510"
     CDS             <1..882
                     /locus_tag="VGB85_16510"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEY0135689.1"
                     /translation="TANHGRDKGAWSDPPKLVGNAWKSLQTRFDRIVDKNKAIEKSNT
                     VVHLGTLGTGNHFIEICLDERQHVWVMLHSGSRGVGNRIGSHFIALAKREMERWKISL
                     PDQNLAYLPEGSEHFADYVEAVEWAQDFARQNRELMMAATLKALGEAKGIRPFELGDM
                     AVNCHHNYVAREHHYGKDVLVTRKGAVRAAAGDLGIIPGSMGTRSYIVRGKGNPESFN
                     SCSHGAGRAMSRTEAKKRFTLADHAAATAGIECRKDEDVIDETPAAYKDIDAVMEAQK
                     DLVEVVHTLKQVVCVKG"
     gene            889..1926
                     /gene="rtcA"
                     /locus_tag="VGB85_16515"
     CDS             889..1926
                     /gene="rtcA"
                     /locus_tag="VGB85_16515"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF003246.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HEY0135690.1"
                     /translation="MAEAKDRIEIDGSQGEGGGQILRSALTLSLVTGRPFRLHRIRAR
                     RVKPGLLRQHLTAVRAATTIGAADVTGDELGSTELLFTPGNVSHGEYTFAVGSAGSVT
                     LVLQTVLWPLLLAPGRSRLTFEGGTHNPMAPPFEFIAEVFLPLLRRMGAKVDATLVRH
                     GFYPAGGGSFHVDIEGGHPLAPLEIFSRGALVRRKARALLAHLPKSIGDREIRVVRNE
                     LGLEREEVRVVDVDSAGPGNALMVWIEAENCTELFCAFGERRVSAEQVAARVVAEVRA
                     HLEADAPVGPHLADQLLIPLALVGRGGYQSYALTGHTLTNMEILARFLPVKFTVTDEG
                     GGRSRVEAHPA"
     gene            complement(1967..>2231)
                     /locus_tag="VGB85_16520"
     CDS             complement(1967..>2231)
                     /locus_tag="VGB85_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135691.1"
                     /translation="LGEHLDPEYTDDSKHSLGRTELRVIPMRYDELIERAERRMFHLY
                     RQLDKTHFTTAQVREFLGPENGSPLLSYSGVDGLGWIEAADSK"
BASE COUNT          389 a          725 c          759 g          358 t
ORIGIN      
        1 accgccaacc atggccgcga caagggtgcc tggtcggacc cgcccaagct cgtcggcaac
       61 gcctggaagt cgctgcagac ccgcttcgat cgcatcgtcg acaagaacaa ggcgatcgag
      121 aagtcgaaca cggtcgtgca cctcggcacg ctcggcaccg gcaaccattt catcgagatc
      181 tgcctcgatg aacgccagca cgtgtgggtg atgctgcact ccggctcgcg cggcgtcggc
      241 aaccggatcg gctcgcactt catcgcgctc gccaagcggg agatggagcg ctggaagatc
      301 tcgctgccgg accagaacct cgcgtatttg ccggagggct cggagcactt cgcggattac
      361 gtcgaggcgg tcgagtgggc ccaggacttc gcccggcaaa accgcgagct gatgatggcc
      421 gcgacgctca aggcgctcgg cgaggccaag ggcatccgcc cgttcgagct cggcgacatg
      481 gcggtcaact gccaccataa ctacgtcgcg cgcgagcacc attacggcaa ggacgtgctg
      541 gtgacccgca agggcgcggt gcgggccgcg gccggcgacc tcgggatcat cccgggcagc
      601 atgggtacgc ggtcgtacat cgtccgcggc aagggcaacc cggagagctt caactcgtgc
      661 agccacggcg cgggtcgggc gatgtcgcgc acggaggcga agaagcgctt cacgctggcg
      721 gaccacgcgg cggcgacggc gggcatcgag tgccgcaagg acgaggacgt gatcgacgag
      781 acgccggcgg cctacaagga catcgacgcg gtcatggagg cgcaaaagga cctcgtggag
      841 gtcgtgcaca cgctcaagca agtggtgtgc gtgaagggct gaatcaagat ggcggaagcg
      901 aaggacagga tcgagatcga tggctcgcag ggcgagggcg gggggcagat cctccgctcc
      961 gccttgacgc tctcgctcgt caccggccgg cccttccgcc tgcaccgcat ccgcgcccga
     1021 cgcgtcaagc cgggcctgct gcgccagcac ctcacggccg tccgcgcggc gaccacgatc
     1081 ggcgccgccg atgtcaccgg cgacgagctc ggcagcaccg agctgctgtt cacgccgggc
     1141 aacgtgtcac acggcgaata taccttcgcg gtcggcagcg ccggctcggt cacactggtg
     1201 ttgcagacgg tcctgtggcc gctgctgctc gcgcccggcc gctcgcgcct gaccttcgag
     1261 ggcggcaccc acaacccgat ggccccgccc ttcgaattca tcgccgaggt gttcctgccg
     1321 ctgctgcgcc gcatgggcgc gaaggtcgac gcgacgctgg tgcgccacgg gttttatccc
     1381 gcgggcggcg gcagcttcca cgtcgacatc gagggcggcc acccgctcgc cccgctcgag
     1441 atcttctcgc gcggggcgct cgtgcgccgc aaggcccgcg cgttgctggc acatttaccg
     1501 aagtcgatcg gcgaccgcga gatccgggtc gtgcgcaatg aattggggct cgagcgcgag
     1561 gaggtccgcg tcgtcgacgt cgacagcgcc ggcccgggaa atgcattgat ggtctggatc
     1621 gaggccgaga attgcacgga gctgttctgt gccttcggcg agcggcgcgt cagcgcggag
     1681 caggtcgccg cgcgcgtggt ggccgaggtc cgcgcgcacc tcgaggccga cgcgcccgtc
     1741 gggccgcacc tggcggacca gttgttgatc ccgctggcgc tggtcgggcg cgggggatat
     1801 cagtcgtatg cgctgaccgg gcacacgctg acgaacatgg agatcctggc gaggttcctg
     1861 ccggtgaagt tcacggtcac ggacgagggc ggcgggcgca gccgcgtcga ggcccacccg
     1921 gcctgagcgc gcggcctttt ctaaagatac aacgtctcaa gcgatcctat ttagaatccg
     1981 ctgcttcaat ccatccgagt ccgtcgacgc cggaatagct gaggagggga ctaccatttt
     2041 ccgggccgag aaattcacgc acttgagcgg ttgtgaaatg tgtcttatcc agttgtctgt
     2101 aaaggtggaa catccgccgt tctgcccgtt caatcagctc atcgtacctc attggaataa
     2161 ctcggagttc agtccgtcca agactgtgct tcgagtcatc agtatactca gggtcaaggt
     2221 gttctccaag c
//
LOCUS       DASWNJ010000975         2607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_229580, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000975 DASWNJ010000000
VERSION     DASWNJ010000975.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229580"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VGB85_16525"
     CDS             <1..205
                     /locus_tag="VGB85_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135692.1"
                     /translation="DETKGRMYREQMLFAAATGVDCRGFFDKDHILQYLPATAIMERF
                     LESDALARFAPGERYFFGHRIPG"
     gene            complement(328..1203)
                     /locus_tag="VGB85_16530"
     CDS             complement(328..1203)
                     /locus_tag="VGB85_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135693.1"
                     /translation="MPILRARGRASHVLFLATLVGLCAPACKGDDECQAKLAGMRRTF
                     QAIPTGAATQQVALIDPDFPLLTSSQGEALRTTGPVVRLNADGSASIDRGKPSFDLEA
                     VVAEAADVLTRPGHDKVLYLAIPPGVALGPHSPLIRRLGDRAPLRVLVRDAGGTPAAP
                     TPSPAMATRLAALRGADASQKATQMAEAMRATAGECKPLAESFESLAGHAPDKRQGAL
                     INGALAGAEQCGCDKVDVDGLAALIWTLTMGDGPPLRWLPLLPGPAGLKLPADGRTGD
                     LVRLLEAGPAIALPG"
     gene            1270..2214
                     /locus_tag="VGB85_16535"
     CDS             1270..2214
                     /locus_tag="VGB85_16535"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0135694.1"
                     /translation="MSDRDVHVVFGTAALGQAIMRQLVAAGKSVRMVSRSGKLDAPAG
                     VEVVKADASVPAEAVAASKDAAVLYHTAAPDYTKWAELYPPIQRGMIAAAAASGARLV
                     SAESVYAYGPVNGPMTEDLPYAPTTRKGKIRVEMSKLLMDAHASGQIKAAIARAPDFY
                     GPKATTTTIYGDRVFYPAIAGKKVSVMGKLDVPHTFIFLDDFARGMITLGAHEEAMGQ
                     VWHLPCAPTLTQRELLTLIFQAAGKEPRVGEAPGWVFKLASPFVPIMRELAELLYQWE
                     RPYLFDHGKFERAFGAHVTPHERAVAATVAWFQAHPQK"
     gene            2264..2488
                     /locus_tag="VGB85_16540"
     CDS             2264..2488
                     /locus_tag="VGB85_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135695.1"
                     /translation="MRVMIVLLLSALTLAEARADVTPEPGRPRWDEYPPPPPPPPPEP
                     ALPVLVTAALALAVCGGLALRRPGLARGTA"
BASE COUNT          365 a          917 c          962 g          363 t
ORIGIN      
        1 ggacgagacg aagggtcgta tgtaccgcga gcagatgctg ttcgccgcag caacaggcgt
       61 ggactgccgc ggtttcttcg acaaggatca catcctgcaa tacctgcccg cgaccgcgat
      121 catggagcgg ttccttgaga gcgacgcgct cgcacgattc gcgccagggg aacggtactt
      181 cttcggccac cggatcccgg gctagtccgc cccccgagcg tcaccaagat cggggcgccg
      241 ggaaacatcg tcaataactt gtattggtga tgtgatccac ctaccgattc cgtccaaacc
      301 acgcaccgga gccgtacaca acgaccacta ccccggcagc gcgatcgccg gtccggcctc
      361 gagcaaacga acgaggtcac cggtgcggcc gtcggcgggc agcttcaacc cggcggggcc
      421 cgggagcagc ggtagccagc gcagcggcgg gccgtcgccc atcgtcaggg tccagatcag
      481 ggcggcgagg ccgtcgacgt cgaccttgtc gcagccgcac tgttcggcgc cggcgagggc
      541 cccgttgatc agggcgccct ggcgcttgtc gggtgcgtgg ccggcgagcg actcgaagga
      601 ctcggcgagc ggcttgcatt cgccggcggt ggcgcgcatc gcctcggcca tctgcgtcgc
      661 tttttgactg gcatcggcgc cgcgcagggc cgcgaggcgg gtcgccatcg ccggtgaggg
      721 ggtcggggcg gccggggtgc cgccggcgtc gcggacgagc acgcgcagcg gggcgcggtc
      781 gccgaggcgg cggatgagcg ggctgtgcgg gccgagggcg acgcccggcg ggatcgccag
      841 gtagaggacc ttgtcgtggc cggggcgggt caaaacgtcg gcggcctcgg cgaccaccgc
      901 ctcgagatcg aaggagggct tgccgcggtc gatgctcgcg ctgccgtcgg cgttgaggcg
      961 cacgaccgga ccggtggtgc gcagcgcctc gccctgcgac gaggtcagca gcgggaagtc
     1021 gggatcgatc agcgcgacct gctgggtcgc cgcgcccgtg gggatcgcct ggaaggtgcg
     1081 ccgcatcccg gccagcttgg cctgacactc gtcgtcgccc ttgcaggcgg gcgcgcacag
     1141 gccgaccagc gtggcgagga acaggacgtg gctggcgcgt ccacgcgcgc ggaggatcgg
     1201 catcctccgg atgttaaacg gcccctgccc tcgctgtcga gcgccgggcg cccgtgttat
     1261 cccgttggca tgagcgatcg ggatgtccac gtggtgtttg gaacggccgc gctcggccag
     1321 gcgatcatgc ggcaattggt ggccgccggg aagtcggtcc gcatggtcag tcgcagcggc
     1381 aagctcgacg cgccggccgg ggtcgaggtc gtgaaggccg acgcgtcggt gcccgccgag
     1441 gcggtcgcgg ccagcaagga cgcggcggtg ctgtatcaca cggcggcgcc ggactacacc
     1501 aagtgggccg agctctaccc gccgatccag cgcggcatga tcgcggcggc cgcggcgagc
     1561 ggcgcgcggc tggtcagcgc cgagagcgta tacgcctacg ggcccgtgaa cgggccgatg
     1621 accgaggacc tgccgtacgc gccgacgacc cgcaagggca agatccgcgt cgagatgtcg
     1681 aagctgctga tggacgcgca cgcgtccggc cagatcaagg cggcgatcgc ccgcgcgccc
     1741 gacttctacg ggcccaaggc gacgaccacc accatctacg gcgaccgcgt gttctacccg
     1801 gcgatcgccg gcaagaaggt gtcggtgatg ggcaagctcg acgtgccgca caccttcatt
     1861 tttctcgacg acttcgcccg cggcatgatc accctcggcg cgcacgagga ggcgatgggc
     1921 caggtgtggc acctgccgtg cgcgccgacg ctgacgcagc gcgagctgct gaccctcatc
     1981 ttccaggccg ccggcaagga gcccagggtc ggcgaggcgc cgggctgggt gttcaagctc
     2041 gccagcccgt tcgtcccgat catgcgcgag ctcgccgagc tgctgtacca gtgggagcgg
     2101 ccctacctgt tcgaccacgg caagttcgag cgggccttcg gagcccacgt gacgccgcac
     2161 gagcgcgccg tcgcggcgac cgtcgcgtgg ttccaggccc acccgcaaaa gtgacgccgt
     2221 tcaccccgcg gcgaggatgc tcgcgtgggg tatcgtgagc ggcatgcgcg tcatgatcgt
     2281 gctcctcctg tcggcgctca cgctcgccga ggcccgcgcc gacgtcacgc cggagccggg
     2341 gcgcccgcgc tgggacgagt atccgccgcc gccgcctccc ccgccgcccg agcccgcgct
     2401 gcctgtgctc gtgaccgccg cgctcgcgct cgcggtgtgt ggtgggctcg cgctgcgccg
     2461 gccgggcctc gcccggggga ccgcgtgagc gccccggcgc ccgcgtactt cgtccgcctg
     2521 gcgcgtccgg gcgagccccc acgcgcgccc gccggggccc agccgtcacc acgcgtccgc
     2581 ccgtcgtcgt cgtgggtcac gtgggcg
//
LOCUS       DASWNJ010000976         1938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_370150, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000976 DASWNJ010000000
VERSION     DASWNJ010000976.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370150"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VGB85_16545"
     CDS             complement(<1..446)
                     /locus_tag="VGB85_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135696.1"
                     /translation="MSVLFTTLALALLAAPPSPEATHRQLVFAGSLALGLGVLYPIPS
                     GELGLFLGSELRPRRPRAGVPRRAALGYHGTVSAGYADLHYAASPRDRSLTGILFHRH
                     GLMALGLGGRRGRLFHGIGGAVVFGDTAIIGVEVEGKLGWVFARSRA"
     gene            591..>1938
                     /locus_tag="VGB85_16550"
     CDS             591..>1938
                     /locus_tag="VGB85_16550"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0135697.1"
                     /translation="MPTSPLTLVCTDPATGARHGELPVMDRAAVVAAVDRGRAAQPAW
                     AATTFAERRAVLKDILAAVVARQVEISALAVHDSGKTMVDAAMGEIFPVCEKLRYTIA
                     HGEKDLRPQARASGLLPHKTARVEYLPLGVIGVISPWNFPFHNFFCPVIPALFAGNAV
                     VIKVSELASWSSLKYLEIFTEVLRARGHSTDLVQVVTGYGETGSALVTSGVDKIFFTG
                     SPQNGRKVMAAAAETLTPVILELGGKDPMIICDDADIDQAASTALFGVFNACGQMCVG
                     AERLYVFSSVYDAFLARVLAGAQALRQGPPNGGEFDLGAMTMPRQLEIIQALIDDAVS
                     KGARVLTGGKPRTELGTNFYPPTILVDVDHSMRIMKEEHFGPVMVIMRVTSEADAIAR
                     ANDCAYGLGSSVFTRDPKRARRMARAIQAGMTTVNDFGLAYMIQSLPFGGVKISGFG"
BASE COUNT          288 a          703 c          677 g          270 t
ORIGIN      
        1 gcccgcgagc gcgcgaacac ccagccgagc ttgccctcga cctccacgcc gatgatcgcg
       61 gtgtcgccga acaccacggc gccgccgatg ccgtggaaca ggcgcccgcg ccggccgccg
      121 aggccgagcg ccatcagccc atggcgatga aacaggatcc ccgtgaggct gcggtccctc
      181 ggagaagcgg cgtaatgcag gtcggcgtag ccggcgctga cggtgccgtg ataaccgagc
      241 gcggcgcgcc gcggcacgcc ggcgcgaggt cggcgcggtc gtagctcgct gccgaggaac
      301 aggccgagct cgcccgaggg gatcgggtag agcacgccga gaccgagcgc gaggctgccc
      361 gcgaacacga gctggcggtg cgtcgcctcg ggcgagggcg gcgcggcgag cagcgcgagg
      421 gccagggtcg tgaacaggac agacatcgcg cggaccgggc gcgcgcaccg gaccccgtat
      481 cttggcccgt gccgtgatcc ggctcaagtc gaaccgcgag ggcgccggtc cgtcgcggcc
      541 ggccgggagg gtagggtgcg cccgccatgt cgaacctcgc cgcctccccc gtgcccacct
      601 ctcccctgac gctcgtgtgc accgaccccg cgaccggggc gcggcacggc gagctgccgg
      661 tgatggaccg cgccgcggtc gtcgccgcgg tcgaccgcgg tcgcgcggcg cagccggcct
      721 gggccgcgac cacgttcgcc gagcgacgag ctgtcctcaa ggacatcctg gccgccgtcg
      781 tcgcgcgcca ggtcgagatc tcggcgctcg cggtgcacga ctccggcaaa acgatggtcg
      841 acgccgcgat gggcgagatc tttccggtct gcgagaagct gcgctacacg atcgcccacg
      901 gcgagaagga cctgcgcccg caggcgcgcg catcgggcct tttgccgcac aagacggcgc
      961 gcgtcgagta cctcccgctc ggcgtcatcg gcgtgatcag cccctggaac ttcccgttcc
     1021 acaacttctt ttgcccggtg atcccggcgc tgttcgccgg caacgcggtc gtcatcaagg
     1081 tctcggagct ggcgagctgg tcgtcgctca agtacctcga gatcttcacc gaggtgctgc
     1141 gcgcccgcgg tcactcgacc gacctcgtgc aggtcgtgac cggctacggg gagaccggct
     1201 cggcgctggt gacctccggc gtcgacaaga tcttcttcac cggttcgccg caaaacggcc
     1261 gcaaggtgat ggccgcggcc gccgagacgc tgaccccggt gatcctcgag ctcggcggca
     1321 aggacccgat gatcatctgc gacgacgcgg acatcgacca ggccgcctcg accgcgctgt
     1381 tcggggtgtt caacgcctgc ggccagatgt gcgtcggcgc cgagcgcctg tacgtgttct
     1441 cctccgtgta cgacgccttc ctcgcgcgcg tgctcgccgg ggcgcaggcg ctgcgccagg
     1501 gcccgccgaa cggcggcgag ttcgacctcg gcgcgatgac gatgccgcgc cagctcgaga
     1561 tcattcaggc gctgatcgac gacgcggtca gcaagggcgc ccgcgtgctg acgggcggca
     1621 agccgcgcac cgagctcggc accaacttct atccgccgac gatcctggtc gacgtcgacc
     1681 actcgatgcg gatcatgaag gaggagcact ttggcccggt gatggtgatc atgcgcgtca
     1741 cctccgaggc cgacgcgatc gcccgcgcca acgactgcgc ctacggcctc gggtcgagcg
     1801 tgttcacccg cgacccgaag cgcgcccgcc ggatggcccg cgcgatccag gccggcatga
     1861 cgaccgtcaa cgacttcggc ctcgcgtaca tgatccagtc gctgcccttc ggcggcgtca
     1921 agatctcggg ctttggca
//
LOCUS       DASWNJ010000977         6840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_583396, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000977 DASWNJ010000000
VERSION     DASWNJ010000977.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583396"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VGB85_16555"
     CDS             <1..997
                     /locus_tag="VGB85_16555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135698.1"
                     /translation="ARGSMFFAPCTLAAVSLVADLGDHVELRWTGPETCPEAEFSAAL
                     ARHVGARASAGAALPVQADVRREDGRWHLDLRVLSDGSESRRLTADSCEVVVDAAAFI
                     VAQALVGASVPPPPEPAPAIEPAPGPLASAPLEATETVFEDAVAVSVATPRPRPQASL
                     HAALRLRGGLSGVGLPGLQPAFGVVAGLVAARWRVELTGIGRLPARTAAGPGTARLAM
                     WAVGARGCAVLRARRVEFPLCAGAEVGQVIGRSEGLLQNGGAAVVWGAVTASPGLAWA
                     PRPWLALVLEAELAVALVRHDWVIRGLPAFASWLGPVDVRGFAGIELRPGRIKKP"
     gene            1061..1597
                     /locus_tag="VGB85_16560"
     CDS             1061..1597
                     /locus_tag="VGB85_16560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0135699.1"
                     /translation="MVRGADARALVAALYDAHADFVVRVARQLGAPAAELEDIVHDVF
                     LVVHRRHGEYSAARGSQRAWLYGICRNLVFHHLRGRTRAELRLRQLPEPAPRPGPDEV
                     LAQARALAAVQSFLDGLDEGRRMVFALIEIEGMAAPEVAQALDLNLNTVYSRLRLARK
                     QFEAHLHRLHRGTGASHG"
     gene            2013..2261
                     /locus_tag="VGB85_16565"
     CDS             2013..2261
                     /locus_tag="VGB85_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135700.1"
                     /translation="MRQARAAIDAGAPEAALRALDKHAQEFAAGQMLEDRLRLRIEAT
                     CAVGRAEQARAQADEFLRDHPGSPHAARVRELCRDDLP"
     gene            2338..3123
                     /locus_tag="VGB85_16570"
     CDS             2338..3123
                     /locus_tag="VGB85_16570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135701.1"
                     /translation="MLIVGCDGKDGVLTAAVQEPGSTGDATTTAVTTGDSPTTGDSTT
                     TGDVAPLVPEPCAPNNAQCPAEAVCCSDDPATVGGKLPNYFTGTVDASYDVPIFSNDN
                     NGLGTSGQCIEVGGFATPMNNGCPVPCNPTWDPARIDEICGIGEIVTCCQTAAVDPDR
                     DCIIDPQTERWRTVTGVDILAGLTSWGDLHATNQDPYGQGCGIFTAGGNGMIDPSEQP
                     ALEDCYAQLTVADQRGFCFAGECPCQEDPCDMKNPDWTPRCAG"
     gene            complement(3162..3392)
                     /locus_tag="VGB85_16575"
     CDS             complement(3162..3392)
                     /locus_tag="VGB85_16575"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0135702.1"
                     /translation="MQHIVLDSYNLETAERRLCVEALTTAGNIVGAAKLLGLTRHALK
                     RRIIKLGISWSRGAGRILDARPEATTTPAHPA"
     gene            complement(3503..3937)
                     /locus_tag="VGB85_16580"
     CDS             complement(3503..3937)
                     /locus_tag="VGB85_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135703.1"
                     /translation="MIRLLLVLLVLGPLGCNYATCADADASFVLPAPGDVVELTVHAC
                     GPERNGMELIVSLGADRAPEETLAIEVDGLGVHLDARDDGWISEDIGSSASSADCEPG
                     RRITIRRLDSDDTVRISGTVAVAMSAPGRETCTADIVVIPPA"
     gene            complement(3934..4392)
                     /locus_tag="VGB85_16585"
     CDS             complement(3934..4392)
                     /locus_tag="VGB85_16585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135704.1"
                     /translation="MPPRPRQLVAYFIVGAALALLPAACICDCDTLECDAANVALELP
                     PGQAYTVHITACIDGFDPATADELGPGSLGLWFQGEGDGAVTLAIEGLDVTPDPWAIS
                     ASTEYSQALCDDGMTVILSNTGTTPVVGVLRVDVHSGDDGEACDLRLEQP"
     gene            complement(4451..5686)
                     /locus_tag="VGB85_16590"
     CDS             complement(4451..5686)
                     /locus_tag="VGB85_16590"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0135705.1"
                     /translation="MTATATRTAPTPSFDFTTPAFRSDPYPFYAQLRRDMPIMRLKQG
                     RRGESFYVSRYDDVMVLLRDVERFASDKRTAGHKVSWLETRLSLGVTESMVMRDGVDH
                     RRLRTLAHKAFTPGRVAALEQRIAQLTDSLLDAADARGNLEMISGLALPLPVAVISDM
                     MGVHERYRPHFHRWMKGILELDGAGWLELLTNYPSMVKLNRFLRELIAERRNNKGDDL
                     LSAMIEAEEAGERLSFDELVASTLIILLAGHETTVNLIGNGMLALLENPEQLGRLRAQ
                     PELLDAAVEEMLRYTSPVQLNAPRYACADTELCGVTIPKGAAVCPIIGAANHDEAQFE
                     DPERFDIGRRPNRHVAFGFGPHFCLGAPLARLEAKIVFRGIVQRYSDIRLAVPRSELT
                     WRRSQSVRGLTALPLQVRR"
     gene            5843..6490
                     /locus_tag="VGB85_16595"
     CDS             5843..6490
                     /locus_tag="VGB85_16595"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0135706.1"
                     /translation="MPGKLQKQRRSKVRPLERGEPVIRKVLAAALTEVARAGYHGLRI
                     EDVATRAGVNKTTVYRRWPSKQELVRDALLSITTETFSVPDTGSLRTDLLAIARRNAA
                     LAAKPEHQGVFRIFAAEGEDAELAAIVRSLRQAFEAVPREVLAAAEARGEVRPGIDYS
                     LLFGVLGATVHWWLLFDRMPVDEPLLQRLIDMLLDGALAQGRVTRSSGRAGAAAT"
     gene            6654..>6840
                     /locus_tag="VGB85_16600"
     CDS             6654..>6840
                     /locus_tag="VGB85_16600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135707.1"
                     /translation="MFVSSNKTAFLAEVFVATDKLGVKSAVVVVRATFAVGRDGATTA
                     SKDQAPFVFADAHYGDPQ"
BASE COUNT         1022 a         2333 c         2439 g         1046 t
ORIGIN      
        1 tgctcgcggt tcgatgttct tcgccccgtg cacgctcgcc gcggtgagcc tcgtcgccga
       61 cctgggcgat cacgtcgagc tgcgctggac gggcccggag acgtgtcccg aggccgaatt
      121 ttccgcggcg ctcgcccgcc acgtcggcgc gcgagcgtcg gccggcgcgg cgttgccggt
      181 ccaggcggat gtccggcgag aagatgggcg ctggcatctc gacctccggg tcctcagcga
      241 tggttcggag agccggaggt tgaccgcgga cagctgcgag gtcgtcgtcg acgcggcggc
      301 gttcatcgtc gcccaggcgc tcgtcggggc gtcggtgccg cctccaccgg agccagcgcc
      361 cgcgatcgaa cccgcacccg gaccactcgc gagcgcgccg ctcgaggcga cagagacggt
      421 cttcgaggat gctgtcgccg tgagcgtggc gacgccacga ccgcgcccgc aggcctcgct
      481 tcacgccgcg ctgcggctgc gtggtggcct gtccggggtc ggtctgccgg gcttgcagcc
      541 ggcgttcggc gtcgtcgcgg ggctggtcgc ggcgcgctgg cgggtcgagc tcacgggcat
      601 cggtcgcttg cctgcacgca ccgccgcggg tcccggcacg gcgcggctgg cgatgtgggc
      661 cgtcggggcg cgcggttgcg cggtgctccg ggcccggcgc gtcgagtttc cgctgtgtgc
      721 cggcgccgag gtcggccagg tgatcgggcg cagcgagggc ctgctgcaaa acggcggcgc
      781 tgccgtggtg tggggggcgg tcaccgcgag ccccggtctc gcgtgggcgc cccgaccgtg
      841 gctcgcgctc gtgctcgagg cggagctcgc ggtcgcgctc gtccgccacg actgggtcat
      901 ccgtggactc ccagcttttg cgagctggct cgggcccgtc gacgtccggg gattcgccgg
      961 gatcgagctg cgccccggac ggatcaaaaa accgtgacgg atcccgcgac cggcggggaa
     1021 gtcgtacgtg ccatcgcccc gagagcgcgc cgaggaaccg gtggttcgcg gcgctgacgc
     1081 gcgtgcgctg gtggcggcgc tctacgatgc acacgcggac ttcgtggtcc gggtcgcacg
     1141 ccagctcggg gcgccggcgg ccgaattgga ggacatcgtg catgacgtgt tcctcgtggt
     1201 ccatcgccgt cacggcgaat actccgcggc gcgcggctcc cagcgcgcgt ggctatatgg
     1261 catctgccgc aacctcgtct ttcatcacct gcgcgggcgc acccgggccg agctgcgtct
     1321 acggcagctg ccggagcccg cgccgcggcc gggtcccgac gaggttctgg cccaggcccg
     1381 cgcgctggcg gccgtgcaga gcttcctgga tgggctcgac gagggccggc gcatggtctt
     1441 tgcgctgatc gagatcgagg gtatggcggc cccggaggtc gcgcaggcgc tcgacctcaa
     1501 tttgaacacc gtgtattcgc ggctgcgcct ggcccgtaaa caattcgagg cccatcttca
     1561 tcgtctgcac cgcgggacag gagccagcca tggatgacct cggtcgagac gcacgcgagg
     1621 cattgcgccg ataccacgcc aaagccgcgc tcgacccgac ggcgcatgag cgcgtgtggc
     1681 agcgcatcga gcggtccctc gaccacgcgg aggctccgtc ggcgcgtcgc gggcccgggc
     1741 ggttcatcct cgtgccgatc ttgctcggcc tggcagccag cgtgctggcc atatgcgacg
     1801 tacgcggcag gctggagagc tcgcggcgcg tcgatgctgc gatatatcag gcggcgcccg
     1861 ggtcggtcgg cacgctgcag ccgcgagcac cggtggcgcc ggcatccgtc gtccgcgagc
     1921 cggcgccgcc gcctgctcgc ccatcgccga ggatcgacgc accaccgacc acgaccctgg
     1981 aagatcgggg cctcgccgcc gagatcgtat atatgcgcca ggcccgggcg gcgatcgacg
     2041 ccggcgcccc tgaagctgcc ctgcgcgcgc tcgataagca cgcccaggaa ttcgcggccg
     2101 gccagatgct ggaagatcgc ctgcgcctgc gcatcgaggc cacatgtgcg gtgggacggg
     2161 cggaacaggc gcgcgcgcag gccgacgaat ttctccgcga tcatccgggc tcgccccacg
     2221 ccgcacgtgt ccgcgagtta tgccgcgatg atcttccgtg acggatcgcc ggcccggcgg
     2281 gaaatctcct tcatgctccg ctcccaccgt cctatgatct gtctcggttt cgcacttttg
     2341 ctcattgtcg gctgcgacgg caaggacggg gtgctcaccg ccgccgtgca ggagcccggg
     2401 tcgaccggcg acgcgacgac cacggctgtc accaccggcg attcgccgac caccggtgac
     2461 tcgacgacca cgggcgatgt cgcgccgctg gtccccgagc cgtgcgcgcc gaacaacgca
     2521 caatgccccg ccgaggccgt ctgctgctcg gacgatcccg cgaccgtcgg cggaaagctc
     2581 ccgaactatt tcaccgggac cgtcgacgcc tcgtacgatg tcccgatctt ctcgaacgac
     2641 aacaatgggc tcggtacatc gggccagtgc atcgaggtcg gcggatttgc caccccgatg
     2701 aacaatggtt gccccgtgcc ctgcaacccg acctgggacc cggctcgcat cgacgagatc
     2761 tgcggcatcg gcgagattgt cacctgctgc cagaccgccg cggtcgaccc ggaccgggac
     2821 tgcatcatcg atccgcagac cgagcgctgg cggacggtca ccggcgtcga catcctcgcg
     2881 ggcctcacga gctggggcga cctccacgcc accaaccagg acccgtatgg ccagggctgc
     2941 gggatcttca ccgcgggtgg caacgggatg atcgacccgt cggagcagcc agcgctcgag
     3001 gattgttatg cgcagctcac cgtcgccgac cagcgcggct tctgcttcgc cggcgagtgc
     3061 ccgtgccagg aggacccgtg cgacatgaag aaccccgact ggaccccgag gtgcgccggc
     3121 taaaccggtc gcgcacctcc tcgtcagcag gcggccgcgg ctcaggccgg gtgcgccggc
     3181 gtcgtcgtcg cttccggacg ggcgtcgagg atccggccgg cgccgcgcga ccacgagatg
     3241 ccgagcttga tgatgcgtcg cttcaacgcg tgacgggtga gcccgaggag cttggccgcc
     3301 ccgacgatgt tgccggcggt ggtcagcgcc tcgacgcaga ggcggcgttc ggcggtctcg
     3361 aggttgtagc tgtcgaggac gatgtgttgc atgccgaggt ttgtgccacg cctgcttgac
     3421 ctggtgcaga tcgagcgcga ggcaaaaaaa tggccctccc aggagcgacg gcgcgccgcg
     3481 caatcgcgcg cgggtgtgtc cattacgcgg gcgggatgac gacaatgtcg gcggtacagg
     3541 tctcgcgtcc gggggccgac attgcgaccg cgacggtgcc ggagatgcgg acggtgtcgt
     3601 cgctgtcgag gcggcggatc gtgatccggc gcccgggctc gcaatccgcg gagctcgcgg
     3661 agctgccgat gtcctcgctg atccagccgt cgtcgcgggc gtcgagatgg acgccgaggc
     3721 cgtcgacctc gatcgccagg gtctcctcgg gcgcacggtc ggcgccgaga gagacgatca
     3781 gctccatgcc gttacgctcg gggccacagg cgtggaccgt cagctcgacg acgtcgccgg
     3841 gagccgggag gacaaaggac gcatccgcgt ccgcgcaggt cgcataattg cagccgagcg
     3901 ggccgaggac cagaaggacg agcagcaggc ggatcatggc tgctccaggc gcaggtcgca
     3961 ggcctcaccg tcgtccccgg aatggacatc gacgcgaagc acaccgacga ccggggtggt
     4021 gccggtattg ctgaggatga ccgtcatacc gtcgtcgcac agggcctgcg aatactcggt
     4081 ggaggcggag atagcccagg gatcgggcgt gacatcgagg ccctcgatcg cgagggtgac
     4141 ggcgccatcc ccctccccct gaaaccacag ccccaagctc ccaggcccga gctcgtcggc
     4201 cgtggccggg tcgaagccgt cgatgcaggc ggtgatatgc accgtgtagg cttgaccagg
     4261 aggtagctcg agggcgacgt tcgcggcgtc gcattccagg gtgtcacagt cgcagataca
     4321 ggcggcgggc agcagcgcca gagcagcgcc gacgataaaa tacgccacga gctgacgcgg
     4381 acggggcggc atgatctcac gagcgtatca cggggcggtg tcggggacat ccgcggacgc
     4441 gcccgcccgg tcagcgccga acctggagcg ggagcgcggt caggccccgg accgactgcg
     4501 agcgccgcca ggtgagctcg gaacgcggta ctgccagccg gatgtcgctg tagcgctgca
     4561 cgatcccgcg aaacacgatc tttgcctcca gccgcgccag cggggcgccg aggcagaagt
     4621 gcggcccgaa gccgaaagcg acgtggcggt tcggccggcg cccgatgtcg aaccgctccg
     4681 ggtcctcgaa ttgcgcctcg tcgtggttcg ccgcgccgat gatcgggcac acagccgcgc
     4741 ccttcgggat ggtcacgccg cacagctccg tgtccgcgca cgcgtaacgc ggagcgttga
     4801 gctgcaccgg gctggtatat cgcagcatct cctcgaccgc ggcgtccagc agctcgggct
     4861 gggcccgcag gcgccccagc tgctcgggat tctccagcaa cgcgagcatg ccgttgccga
     4921 tcaagttgac ggtcgtctcg tgccccgcca ggagaatgat cagggtcgag gcgacgagct
     4981 cgtcgaagct gagccgctca ccggcctcct cggcctcgat catcgcgctg agcagatcgt
     5041 cgcccttgtt gttgcgacgc tcggcgatca gctcccgcaa gaagcgattg agcttgacca
     5101 tgctgggata gttggtgagc agctcgagcc agccggcgcc gtcgagctcg aggatgccct
     5161 tcatccagcg atggaagtgc ggacgatagc gttcgtgcac gcccatcatg tcggagatca
     5221 cggcgaccgg cagcggcagc gcgaggccag agatcatctc caaattgccc cgcgcatcgg
     5281 cggcgtccag caagctgtcc gtgagctgcg cgatccgctg ctcgagcgcg gcgacccgcc
     5341 ccggcgtgaa ggccttgtgt gcgagcgtgc gcagccgccg atgatcgacc ccgtcccgca
     5401 tcaccatcga ttcggtgacg cccagcgaca gccgcgtctc gagccacgac accttgtgcc
     5461 cggctgtgcg cttgtcgctg gcgaagcgct cgacatcccg gagcagcacc atcacgtcgt
     5521 cgtaacgcga gacgtagaag ctctcgccgc ggcgcccttg ttttagccgc atgatcggca
     5581 tgtcgcgccg caactgcgca taaaagggat acgggtcgct acgaaacgcc ggcgtcgtga
     5641 agtcgaacga cggcgtcggc gcggtgcgcg tggcagtggc ggtcatgggc tggtctccag
     5701 gcgagacgga gccgggggcc cggcgcggcc tcgcaacttt tggttgcgat taacccttgc
     5761 acgcctccgg ctcgaccgca acaaagtttt gcgatagctt ttaaaaagaa aagatcggcg
     5821 gtgcacgccg cgctgaaccg ccatgcccgg gaagctccaa aagcagcgca ggtcgaaggt
     5881 gcggccgctc gagcgcggcg agccggtgat ccgcaaggtg ctcgcggccg cgctgacgga
     5941 ggtggcgcgc gcgggttacc acgggctgcg gatcgaggac gtcgccacgc gcgcgggcgt
     6001 caacaagacg accgtgtacc ggcgctggcc gagcaagcag gagctggtgc gcgacgcgtt
     6061 gctgtcgatc accaccgaga ccttctcggt cccggacacc ggctcgctgc gcactgacct
     6121 gctggcgatc gcccgccgca acgccgcgct cgcggccaag cccgagcacc agggagtatt
     6181 tcgcatcttc gccgccgagg gcgaggacgc cgagctggcg gcgatcgtgc gatcgctgcg
     6241 ccaggcgttc gaggcggtgc cgcgcgaggt cctcgcggcc gccgaggccc gcggcgaggt
     6301 ccgcccgggt atcgactact cgctgctgtt cggggtgctc ggcgcgacgg tccactggtg
     6361 gctgctgttc gaccgcatgc cggtcgacga gccgctgctg cagcggctga tcgatatgct
     6421 gctcgacgga gcgctcgcgc agggtcgggt cacccgctcg agcggacgcg ctggcgctgc
     6481 ggcgacctga cgcgctggcg ctcccgggcg gccagttgcg cgcgatcggg tacaggatgt
     6541 cgtcgcgttc ggggtgcttg cgctcgtcct cgagatgtac ggatccgctc gccgcgccga
     6601 cgtgctgcga cgcgactgga ggatctggat gcgcgtcggt acactgacgt gccatgtttg
     6661 tcagcagtaa taagaccgcg tttctcgccg aggtgttcgt ggccacggac aaactcggcg
     6721 tgaaatcggc ggtcgtcgtc gtccgcgcca cgttcgcggt cgggcgtgac ggtgcgacga
     6781 cggcgagcaa ggaccaggcg ccattcgtgt tcgccgatgc ccattacggc gaccctcaga
//
LOCUS       DASWNJ010000978         4165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_123274, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000978 DASWNJ010000000
VERSION     DASWNJ010000978.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123274"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4121
                     /locus_tag="VGB85_16605"
     CDS             <1..4121
                     /locus_tag="VGB85_16605"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0135708.1"
                     /translation="VLGRRDRGDELRLSQRLHGREAAQKVVRAAWERASRGSAELLLL
                     SGPSGVGKSALVEASRGELAARGGSFIAGKCDQLDRDTPYAPLERAFQELVRGVLGEP
                     APVLAALRARLIAALGPNGRVVADIVPEVELVIGPQQPVAELGPAESRNRLNLVFHGF
                     LGAFAAPQRPLTLFLDDLQWADPATLKLLVALLTDPDSGHLLVIGACRDDEVADADPL
                     ALALAQLRSSGCPPLDVPLAPLGDAEIAAFVAETLGSEAVEVAPLAALLREKTGGNPY
                     FVGQLLLGLFQDGLLAFDAGRGGWQWDLDAVGMATGGDILAVVTGKLEALAPETRRVL
                     QFAACIGHRFDSETLASALEQTPAQVERGLEEARRAGLLVADAGAQVHRFTHDRIQQA
                     AHAQIEEAGRTAVHLRVARVLRARLGTEVRDDELFAVVHHENLGAPLISDPGEREGVL
                     RRNVAAGRRARKAAAFEAAAGYFDAATAQLGEDSWDRDHAWTFGVHAEQAECAYLCGR
                     FEPAEAACQRLLIRARSPEQILLLEGRRMEMRIARSCFAEAVEIGITALGSLGICLPR
                     TEEERMAALGGRLAEVESILGQREVATLLDEPRLSDPEIEAKVQVLMILAMAAYYVSA
                     TVYALCAIEQVILSLKHGNSALSPVAYMAYAFVLAALLGSHERAEAFGELALALHARH
                     PDPRVECKTYHMFGTILHFRRPIRVALAHLERARKAGLEHGDFVFASTAVDNSFPYKF
                     AVGMPLSELRGEVAQMLALVHRTRDALSIAFLTIARQLVAGLEGRTRGPSTLDGDGFT
                     EEGHAAALVRDGLYPIACWLHTTRVILCYLHGDMAGAAAAAESAEPLVPSTAGQYFPT
                     ELAFFACLVAASVVADGPEAERRRDELLARNLAWLTLWATSCPENYRHKQWLVEAEVA
                     RLAGRHDEATDGYERAISGARAEGFVRDEAIACERAALFHLEKGRPRISRVYMSDAHA
                     CHVRWGATVKAAALAEQYADLLPDIGALAPITSAGAFTLTTRMGSSGLVDAAALVRAA
                     QALVGEVALEVVLERLVRLVVESAGAQRGVLLLAHDEQLEVAARLDGDEVVVGEPVPL
                     EQATDIAASVVRYVARTREAVVLDDATRDHRFAADPHLGEHRPRSILCLALTQNDRLT
                     GVLYLENNAVSRAFTRARVDLAGLLASLAATAVANAQLYARVEEVTLALRGANESLED
                     EVARRTDELRASNARLTVELAERARAEQARSTLHAQVVRMQQDRLAELSAPILPITDR
                     VVVMPLIGTMDAQRGRQVMEAALMGAREHRAEIVIFDVTGMKMVDAEVADMLAATAGA
                     LKLLGARALVTGLSPEVAWTFIERGEQLGAMKTVATLRAAVALAGTTRRRPG"
BASE COUNT          535 a         1509 c         1547 g          574 t
ORIGIN      
        1 tcgtgctcgg tcgacgcgac cgcggcgacg agctccggct gtcgcagcgg ctgcacgggc
       61 gcgaggccgc gcagaaggtc gtgcgcgcgg cgtgggagcg cgcgagccgg gggtccgccg
      121 agctgctgct gctgtccggc ccgtcgggcg tcggcaagtc ggcgctcgtc gaggcgtcgc
      181 gcggcgagct ggcggcgcgc ggcgggtcgt tcatcgccgg caagtgcgac cagctcgatc
      241 gtgacactcc gtatgcgccg ctcgagcgcg cgttccagga gctcgtgcgc ggcgtgctcg
      301 gcgagccggc gccggtgctc gcggccctgc gcgcgcgcct catcgcggcg ctgggcccga
      361 acggtcgcgt ggtcgccgac atcgtgcccg aggtcgagct ggtgatcggg ccgcaacagc
      421 cggtcgccga gctcggcccg gccgagtcgc gcaaccgcct caacctcgtg ttccacggct
      481 tcctcggcgc cttcgcggcg ccgcagcggc cgctgaccct gttcctcgac gacctgcagt
      541 gggccgaccc cgcgacgctc aagctgctcg tcgcgctgct gaccgacccc gactccgggc
      601 acctgctcgt catcggtgcg tgtcgcgacg acgaggtcgc cgacgccgac ccgctcgcgc
      661 tcgcgctcgc ccagctgcgc agcagcggct gcccgccgct cgatgtcccg ctcgccccgc
      721 tcggcgacgc agagatcgcc gcgttcgtcg cggagacgct cggcagcgag gccgtggagg
      781 tcgccccgct cgcggcgctg ctgcgcgaga agaccggcgg caacccctac ttcgtcggcc
      841 agctgctgct cgggctgttc caggacggcc tgctcgcgtt cgacgcgggt cgcggcggct
      901 ggcagtggga cctcgacgcc gtgggcatgg cgaccggcgg cgacatcctc gcggtcgtca
      961 ccggcaagct cgaggcgctc gcgccggaga cccggcgggt cctgcaattc gcggcgtgca
     1021 tcggccaccg cttcgacagc gagaccctgg cgagcgcgct cgagcagacg cccgcgcagg
     1081 tcgagcgcgg gctcgaggag gcccgccgcg ccggcttgct ggtcgcggac gcgggcgcgc
     1141 aagtacaccg attcacgcat gacaggatcc agcaggccgc gcacgcgcag atcgaggagg
     1201 ccgggcgcac cgcggtgcac ctgcgcgtcg ccagagtatt gcgcgcgcgg ctcggcacgg
     1261 aggtccgcga cgacgagctg ttcgccgtcg tccaccacga gaacctcggc gcgccgctga
     1321 tcagcgatcc cggtgagcgc gagggggtcc tccggcgcaa cgtcgcggcc ggcaggcggg
     1381 ccaggaaggc ggcggccttc gaggcggccg ccggttactt cgacgccgcg acggcgcagc
     1441 tcggcgagga cagctgggac cgcgaccacg cctggacctt cggggtgcac gcggagcagg
     1501 ccgaatgtgc gtacctgtgc gggcgcttcg agccggccga ggccgcgtgc cagcgactgc
     1561 tgatccgcgc gcgctccccg gagcagatcc tgctgctcga gggccggcgc atggagatgc
     1621 ggatcgcccg gtcgtgcttc gccgaggcgg tcgagatcgg catcaccgcg ctcggctcgc
     1681 tcgggatctg cctgccgcgg accgaggagg agcgcatggc cgcgctcggg ggccggctcg
     1741 ccgaggtcga gtcgatcctc ggccagcggg aggtcgccac attgctcgac gagccgcggc
     1801 tctcggatcc cgagatcgag gccaaggtcc aggtattgat gatcctggcg atggccgcat
     1861 attacgtcag cgcgacggta tatgcgctgt gtgcgatcga gcaggtgatc ctgtcgctca
     1921 agcacggcaa ctcggcgctg agcccggtcg cgtacatggc ctatgcattc gtgctcgccg
     1981 cgctgctcgg cagccacgag cgggccgagg cgttcggcga gctcgcgctg gccttgcacg
     2041 cgcggcatcc cgacccgcgg gtcgagtgca agacctatca tatgttcggg acgatcctgc
     2101 actttcgcag gccgatccgc gtcgcgctcg cgcacctcga gcgggcccgc aaggccggcc
     2161 tcgagcacgg cgacttcgtg ttcgcgtcga cggcggtcga caacagcttc ccttacaagt
     2221 tcgcggtcgg catgccgctg tcggagctgc gcggcgaggt cgcccagatg ctcgcgctgg
     2281 tccatcgcac gcgcgacgcc ctgtcgatcg cgttcctgac gatcgctcgc cagctggtcg
     2341 cgggcctcga gggtcgcacg cgcggcccct cgacgctcga cggcgacgga tttacagagg
     2401 aaggccacgc cgcggcgctc gtgcgcgacg ggctgtaccc gatcgcgtgc tggttgcaca
     2461 cgacgcgggt catcctgtgt tatctgcacg gcgacatggc cggcgccgca gcggcggccg
     2521 agtcggccga gccgctggtg ccgagcaccg cgggccagta cttcccgacc gagctggcgt
     2581 tcttcgcctg cctcgtcgcc gcgagcgtgg tcgcggacgg tccggaggcg gagcgtcgcc
     2641 gcgacgagct gctcgcgcgc aacctcgcct ggctgacgct gtgggcgacg agctgccccg
     2701 agaattatcg tcacaagcaa tggctggtgg aggccgaggt ggcgcgcctg gcgggccggc
     2761 acgacgaggc gaccgacggc tacgagcggg cgatctcggg ggcccgggcg gagggcttcg
     2821 tgcgcgacga ggcgatcgcg tgtgagcgcg cggcgctgtt ccacctcgag aaggggcgcc
     2881 cgaggatctc ccgcgtatac atgtccgacg cgcacgcgtg ccacgtgcgc tggggcgcga
     2941 cggtcaaggc ggccgcgctc gccgagcaat acgcggacct gctgccggac atcggcgcgc
     3001 tcgccccgat caccagcgcc ggagcgttca ccctgaccac gcgcatgggg tccagcgggc
     3061 tcgtcgacgc ggcggcgctc gtgcgggccg cccaggcgct cgtcggcgag gtcgcgctcg
     3121 aggtcgtgct cgagcggctc gtgcggctcg tggtcgagag cgccggggcc cagcgcgggg
     3181 tcctgctgct cgcgcacgac gagcagctcg aggtcgccgc ccggctcgac ggcgacgagg
     3241 tcgtggtcgg cgagccggtg ccgctcgagc aggcgaccga catcgccgcc tcggtcgtgc
     3301 gctacgtggc gcgcacccgc gaggcggtgg tcctcgacga cgcgaccagg gaccaccgct
     3361 tcgccgcgga cccccacctc ggcgagcacc gcccgcggtc gatcctgtgc ctggcgttga
     3421 cgcagaacga ccgcctgacc ggggtcctgt acctcgagaa caacgccgtc agccgggcct
     3481 tcacccgcgc gcgcgtcgac ctcgcggggc tgctcgcgtc gctcgccgcg accgccgtcg
     3541 ccaacgcgca gctgtacgcc cgcgtcgagg aggtgaccct ggcgctgcgc ggcgccaacg
     3601 agtcgctcga ggacgaggtg gcgcgccgca ccgacgagct gcgcgccagc aatgcccggc
     3661 tgacggtcga gctcgccgag cgggcgcggg ccgagcaggc gcgcagcacc ctgcacgcgc
     3721 aggtcgtccg catgcagcag gaccggctcg ccgagctctc ggcgccgatc ttgccgatca
     3781 ccgaccgcgt cgtggtgatg ccgctgatcg gcacgatgga cgcgcagcgc ggccgtcagg
     3841 tgatggaggc cgcgttgatg ggcgcgcgtg agcaccgggc cgagatcgtc atcttcgacg
     3901 tcaccggcat gaagatggtc gacgccgagg tcgccgacat gctcgccgcc accgccggcg
     3961 ccctcaagct gctcggcgcg cgcgcgctgg tcaccggcct gtcccccgag gtcgcctgga
     4021 ccttcatcga gcgcggcgaa cagctcggcg cgatgaagac ggtcgccacg ctgcgcgccg
     4081 cggtcgcgct cgcgggcacc acccggcgaa ggccgggcta ggagccggcc acttacttta
     4141 ctgaccgatc ctggtggtca gcgat
//
LOCUS       DASWNJ010000979         2708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_370215, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000979 DASWNJ010000000
VERSION     DASWNJ010000979.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370215"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VGB85_16610"
     CDS             complement(<1..619)
                     /locus_tag="VGB85_16610"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0135709.1"
                     /translation="MGREAESSLADFYFSDSDDPLIPPESFTRWAEEGAWAMAMYEPQ
                     MLGGVAPRMDIMRAGVTSRVVNLSSYNYLGLTMHPEVIEAAQKALRDYGTGACGSPLL
                     SGMTDMHKELEHKLAAFTGREDCIIFNSGFAGGMGTLTGLLRKGDVAILDSKSHLCSV
                     DGAKLSRARLMFFEHNDPNSLREALEQTAGQRRLIVLEGVYSMDGD"
     gene            805..1893
                     /locus_tag="VGB85_16615"
     CDS             805..1893
                     /locus_tag="VGB85_16615"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0135710.1"
                     /translation="MSNRVLVTGASGFIGSHLVEALHERGQEVRAMVRSSSKLTHLEA
                     CGAQRVHAALDDVEAMAAAMAGVEIVYHVAGLTAAFNRVEFDRANAAGTANVFAAARR
                     AGVRRVVYVSSLMAAGPSHADVARREHHAHRVGFTAYGDSKLGGERIAWEAARSGQVE
                     AVIVRPPLVYGPRDTDVLQMIKSANAGIIAQPGLGRPAWLSAIHALDLVQGIALAGER
                     GVPLPSAAQDPHGEHVLAGHGAPHDHVPDHPDEPAGRGIYYFTDGGQHTVTSFGRVAA
                     TALGRKAMALRFPGPAVLTVAAVNTWLGKIRGKAPALSLDKARGSLSPGWWCDDGKAV
                     HTLGYRPQYPLEQGLEHTVRWLRDARWL"
     gene            2098..>2708
                     /locus_tag="VGB85_16620"
     CDS             2098..>2708
                     /locus_tag="VGB85_16620"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF013836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEY0135711.1"
                     /translation="MLRPSVAHIAFDADQFTTLRRDLVTGALSTERARLPTAPEQLAP
                     EDLTALAALDDPTRERLRRRGEAAIVAGEVAALVLNGGMATRFGGAVKGVVPVLPGRD
                     ELSFLAIKLAGLRAVAREVGGRVPVVLMNSFATAAATREHLDAIRWAGMPEDERLSFE
                     QSLLPRVLPDGTPLAASASAKDLEDTHVYAAPGHGDTLRRVRD"
BASE COUNT          378 a          951 c          987 g          392 t
ORIGIN      
        1 cgtcgccgtc catcgagtac acgccctcga gcacgatcag gcgccgctgg cccgcggtct
       61 gctcgagcgc ctcgcgcagc gaattcgggt cgttgtgctc gaagaacatc aggcgcgcgc
      121 gcgagagctt ggcgccgtcg accgagcaga ggtggctctt gctgtcgagg atcgcgacat
      181 cgcccttgcg aagcaggccg gtcagcgtgc ccatgccgcc cgcaaagccg ctgttgaaga
      241 taatacagtc ttcgcggccg gtgaacgcgg cgagcttgtg ctcgagctcc ttgtgcatgt
      301 cggtcatgcc cgacagcagc ggggagccgc aggcgccggt gccgtagtca cgaagggcct
      361 tttgcgcggc ctcgatgacc tcggggtgca tcgtcaggcc gaggtagttg tacgagctca
      421 gattgacgac gcggctggtg acaccagccc gcatgatgtc catccgggga gcgacgccgc
      481 cgagcatctg gggctcgtac atcgccatcg cccacgcgcc ctcctcggcc cagcgggtga
      541 agctctccgg ggggatcagc ggatcgtcgc tgtccgagaa atagaagtct gcgaggctag
      601 attcggcttc gcggcccatg cgcgttccca ggggtgaatg aggggtagtt acagtgaaag
      661 tagcgtgtgt gtcacggaaa ttcagcgact caccgctatt tacacgcgcg ttcaatgcga
      721 ggtcgcgagg gccttccacc gtgcatgctg ctgcgcacca cgggcccttg cgcggcccgt
      781 tggctgcggc tatctcgggg cctcgtgagc aaccgagtcc tcgtcaccgg cgccagcggc
      841 ttcatcggca gtcacctcgt cgaggcgctg cacgagcgcg gacaggaggt ccgggccatg
      901 gtgcgcagca gctcgaagct gacgcacctc gaggcctgcg gcgcgcagcg agtgcacgcg
      961 gcgctcgacg acgtcgaggc gatggcggcg gcgatggcgg gtgtggagat cgtctatcac
     1021 gtggccggtc tgacagcggc cttcaaccgc gtcgagttcg accgcgccaa cgcggccggc
     1081 accgccaacg tgttcgcggc ggcacggcga gcgggcgtgc gccgcgtcgt gtacgtgtcc
     1141 tcgctcatgg ccgcggggcc ctcgcacgcc gacgtcgcgc gccgtgaaca ccacgcgcac
     1201 cgcgtcggct tcacggcata cggcgatagt aagttgggcg gcgagaggat cgcgtgggag
     1261 gcggcgcgca gcgggcaggt cgaggcggtc atcgtgcgcc cgccgctggt ctacgggccg
     1321 cgcgacaccg acgtgctgca gatgatcaag tcggccaacg ccgggatcat cgcccagccc
     1381 gggctcgggc gcccggcgtg gctgtcggcg atccacgcgc tcgacctggt ccagggcatc
     1441 gcgctggccg gcgagcgcgg ggtccccctg ccctccgcgg cgcaggaccc ccacggcgag
     1501 cacgtgctcg ccggccacgg cgcgccgcac gaccacgtgc ccgaccaccc cgacgagccg
     1561 gccgggcgcg gcatctacta cttcaccgac ggcggccagc acacggtgac cagcttcggc
     1621 cgcgtcgccg cgacggcgct cggccgcaag gcgatggcgc tgcgcttccc gggacccgcg
     1681 gtgctgacgg tcgcggccgt caacacgtgg ctgggcaaga tacgcggcaa ggccccggcg
     1741 ttgtcgctcg acaaggcccg cggcagcctg tcgcccggct ggtggtgcga cgacggcaag
     1801 gcggtgcaca cgctcggcta tcgcccgcag tacccgctcg agcagggcct cgagcacacg
     1861 gtccgctggc tgcgcgacgc ccggtggctg tgagcctgtc cgctggaacc tgtcccgagg
     1921 gccgtgtcct ttcggccagg ttccacgcca ctttggtgtg aggcggccag cgaagcgccg
     1981 gtctcgcggg ccgcgcaggc gacgacacgt gtgcggcggt acggcgtccg gcgtgtgaag
     2041 ccggggccgc gccgcgcatg cgacgcccgg ctcgccggct cggttatgct gccggcgatg
     2101 ctccgcccgt ctgtcgctca catcgccttc gacgccgatc agttcaccac gctgcggcga
     2161 gatctggtca ccggcgcgct cagcacggag cgtgcgcggc tgccgaccgc gcccgaacag
     2221 ctcgcgccgg aggacctcac agcgctcgcg gcgctggacg atccgacgcg cgagcgcctg
     2281 cgacggcgcg gcgaggcggc gatcgtcgcc ggtgaggtcg cggcgctggt gctaaatggc
     2341 ggcatggcga cccgcttcgg cggcgcggtc aagggcgtgg tcccggtgct gcccggtcgc
     2401 gacgagctgt cgttcctggc gatcaagctc gcggggctgc gggcggtcgc ccgtgaggtc
     2461 ggcggccggg tcccggtcgt gctgatgaac agcttcgcca ccgccgcggc gacgcgcgag
     2521 cacctcgacg cgatccgctg ggccgggatg ccggaggacg agcgcctgag cttcgagcag
     2581 tcgctgctgc cgcgcgtgct gcccgacggc acgcccctgg ccgcctccgc gagcgcgaag
     2641 gacctcgagg acacccacgt ctacgcggcc cccggccacg gcgacacgct gcgacgcgtg
     2701 cgcgacag
//
LOCUS       DASWNJ010000980         6644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_370244, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000980 DASWNJ010000000
VERSION     DASWNJ010000980.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370244"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..321)
                     /locus_tag="VGB85_16625"
     CDS             complement(148..321)
                     /locus_tag="VGB85_16625"
                     /inference="COORDINATES: protein motif:HMM:NF012522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEY0135712.1"
                     /translation="MARPSRYRCVICSHVYDPAVGDPDSGVAPGTPFEDLRDDWFCPE
                     CGAGKADFEPIED"
     gene            392..3283
                     /gene="fdhF"
                     /locus_tag="VGB85_16630"
     CDS             392..3283
                     /gene="fdhF"
                     /locus_tag="VGB85_16630"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEY0135713.1"
                     /translation="MPDALTPPAPVATVALQIDGQPVSVPAGTTVWDAARSVGVEIPV
                     LCHDPRYRPVGVCRMCVVDVGGRVLAAACVRPCEPGMKVTTTSPAIEEHRAMLTALLL
                     ADQPTAPKQQRPEGNLLNVLADRYGLEAGPSDSRVGPLGLPRGGGRPADDSSPVIGVD
                     HQACILCDRCVRACDEVQNNEVITRSGKGYGTKIAFDLDLPMGSSSCVSCGECMDACP
                     TDALVNKQLAAEIRPPAQLKQVETLCPYCGVGCSVTAHVDEASNKVAWIDGRDSRVSD
                     RRLCVKGRYGFDYASHAQRLTVPLIRKDSSYPKGPLSAAVRQKKGRRPGGLVDDREVL
                     PHFREASWDEALALVASKLKGVRDTHGPRALAGLGSAKCSNEEAYLFQKLVRVGFGSN
                     NVDHCTRLCHASSVAALMETIGSGAVSTTFRDIENSDVALLCGTNATSNHPVAATFFK
                     QAAKRGTKLLVVDPHRPQLADHAYRYVRIKPGTDVAFFNAVMHEIVRRGLVDQSFIRE
                     RTEGYEALQAMVSHYPPEVAARICGVEPQEIIDIAVTWGSANAAICFWGMGVSQHTHG
                     TDNARCLISLVLMTGQVGRPGTGLHPLRGQNNVQGASDAGLIPMVFPDYQSVKDPTIR
                     ARFEAAWGVPLDAQPGLTVVEIIHAALHGDVRGMYIQGENPFISDPNTAKVRKALAAL
                     DFLAVQDIFLTETAEFADVILPASSFFEKTGTYTNTDRRVQIGRPVLKMPGEARLDWQ
                     ITCEIARRMGAPGFDYESVDQVFDEFAALTDSYRGLSYEHLGLEGKLWPCPDPASSDG
                     TVVLFTEDFPSGRGKMVPAEFAPPAELPNPEFPIVLTTGRLLEHWHTGTMTRRAVALD
                     AIEGEPHVDMHPEDMRTLGLADEDWATVTSRRGSITLKVRGSRSPGRGAVFIPFCWRE
                     AAANVLTIDELDPYGKIPEFKFCAVRVERASPGDMHDLDEPETDRSAAE"
     gene            3286..3819
                     /locus_tag="VGB85_16635"
     CDS             3286..3819
                     /locus_tag="VGB85_16635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135714.1"
                     /translation="MVEIFLVSMVALLGGFGFYRLVRAQQEARRYLESGTDAPLQLSH
                     LSSDLAKVARETRTLRISLESPLRAIREFMGTDLTRTEDDVEAVDAALMEATRDIGVW
                     LALVDSLPESERGTLEAMGASPGAVRAALAAEGWAFERSHLRAPGRGNLDDRLEAILR
                     ELERVEKALQVPINPYR"
     gene            3920..4756
                     /locus_tag="VGB85_16640"
     CDS             3920..4756
                     /locus_tag="VGB85_16640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135715.1"
                     /translation="MRLLTCVLSLAILGIAARAEATTCGDRTAPWWDPYATPLYSEDQ
                     PLPIDGQPWQFVDCSSEGVAIGACEFVDAEGLRTPVEVEDVGVELCELRYGAGVRETA
                     SSFIRRFVPPAPLVPGAYTLECEGRGSGSFEIRETDTPAAPPTPLVFTDTHLTRGDDG
                     GCCGGFGDVVELRVADDLPYLHEGGYVEVKYPNGQHLALTRAEDGQFVLPWDQVVIEL
                     TPVSASGARGETVVLDLAEVDGDLAYVPCDVAGRRPSAALWLLAPLAWLYTQGRRRRR
                     CA"
     gene            4753..5379
                     /locus_tag="VGB85_16645"
     CDS             4753..5379
                     /locus_tag="VGB85_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135716.1"
                     /translation="MSPVVLLWVSLGTGSVQAPDGPPTAAQDSAAPASMPSPPAPAPA
                     PAASPGVVAVAYTPYQPQPQRAVPPRRFVVAFLPSLTMGISPRPSFNLAGFVGGRLPG
                     AWALGYQFAFSSGFAERYSRGLLTHRHHVMAMRAFGASGRGFASVGAGAAFLELNPVV
                     EAEGRLGVRFGRKKYGVVAAMMRLGWDIGHRERVPMPQFGIVFGFAVM"
     gene            complement(5446..6573)
                     /locus_tag="VGB85_16650"
     CDS             complement(5446..6573)
                     /locus_tag="VGB85_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HEY0135717.1"
                     /translation="MKVSSQFDGGNIEVVDITRPDDAHLRIRHDSNSEFLQWFNFRVS
                     EAQGVALNLHIDNAGETSYPPGWQDYRAVATYDRQHFFRVPTDYDGKRLTIRHTPTRD
                     VVYYSYFAPYSHERHQDLIARSLALPGVRHELLGHTLEGRDMDLLRIGELGGDRRVCW
                     IVARQHPGETMAEWLMEGLIAHLIDPEDPVARTLRSRAVFFLVPNMNPDGSFRGNLRV
                     NAAGANLNREWQNPTAERSPEILLVRRRMEATGVDFCLDVHGDEALPYNFIAGADGVA
                     SVTQRVRALQVSYEKALMRASPDFQMEHGYDPVPPGQANMTMCTTHVAGHFGCLAMTL
                     EMPFKDTKDTPNEAVGWSPERCRQLGRANLDALLTVLGDLR"
BASE COUNT          961 a         2271 c         2429 g          983 t
ORIGIN      
        1 gcccgcgacc ggcgcgggcg gcgaagctgt cacggcgcca ccgttcgccg gctgcgccgc
       61 gggcccgcac gcgaacatga ggagcgaggc gagggcgagg agccggggca cggggagcac
      121 cgtagcatga cgatcgcccg cgccgactca gtcctcgatc ggttcgaagt cggccttgcc
      181 ggccccgcac tccgggcaga accagtcgtc ccggaggtcc tcgaagggcg tgccgggcgc
      241 aactccgctg tcggggtcgc ccacggcggg gtcatagacg tgtgaacaga tgacgcatcg
      301 gtaacgcgac ggccgggcca tgatgcgccg atcctacacg cgtgacgcgc cgtgaggtag
      361 tggcgtcgcg gtgggctatc atgtgcaggc catgcccgac gccctgacgc ctcccgcccc
      421 ggtagcgacc gttgcgctgc agatcgacgg ccagccggtc tccgtgcccg cgggcaccac
      481 ggtgtgggac gcggcccgct ccgtcggcgt cgagatcccc gtgctgtgcc acgacccgcg
      541 ctacaggccg gtgggggtgt gccgcatgtg cgtggtcgac gtcggcgggc gcgtgctcgc
      601 ggccgcgtgc gtgcgcccgt gcgagcccgg catgaaggtc acgaccacga gcccggcgat
      661 cgaggagcac cgcgcgatgc tgaccgcgct gctgctcgcc gatcaaccga cggcgccgaa
      721 gcagcagcgc ccggagggta atttgttgaa tgtgctggcg gaccgctacg gcctcgaagc
      781 tggcccttcg gacagccgcg tggggccgct gggcctgccg cgcggcggcg ggcgcccggc
      841 cgacgactcg tcgccggtga tcggcgtcga tcatcaggcc tgcatcctgt gcgaccgctg
      901 cgtgcgcgcg tgcgacgagg tccagaacaa cgaggtgatc acccgcagcg gcaagggcta
      961 tggcaccaag atcgcgttcg acctcgacct gccgatgggc agctcgagct gcgtgtcgtg
     1021 cggcgagtgc atggacgcgt gcccgaccga cgcgctggtc aacaagcagc tcgccgccga
     1081 gatccgcccg cccgcgcagc tcaagcaggt cgagacgctg tgcccgtact gcggcgtcgg
     1141 ctgcagcgtg acggcccacg tcgacgaggc gagcaacaag gtcgcgtgga tcgacggccg
     1201 cgacagccgc gtgtccgacc gccgcctgtg cgtcaagggc cgctacggct tcgactacgc
     1261 gtcgcacgcc cagcgcctga cggtgcccct gatccgcaag gacagcagct atccaaaagg
     1321 gccgctgtcg gcggcggtgc gccagaagaa gggccgccga ccgggcgggc tcgtcgacga
     1381 ccgcgaggtg ctgccgcact ttcgcgaggc gagctgggac gaggcgctcg cgctggtcgc
     1441 aagcaagctc aagggcgtcc gcgacacgca cggcccgcgc gcgctggccg gcttaggctc
     1501 ggccaagtgc agcaacgagg aggcgtacct gttccaaaag ctggtgcgcg tcggcttcgg
     1561 cagcaacaac gtcgaccact gcacgcgcct gtgccacgcg tcctcggtcg cggcgctgat
     1621 ggagacgatc ggctcgggcg cggtgtcgac gacgtttcgc gacatcgaga acagcgacgt
     1681 cgccctgctg tgcggcacca acgcgacgag caaccatccg gttgccgcga cattcttcaa
     1741 acaggcggcc aagcgtggca cgaagctgct cgtcgtcgac ccgcaccgcc cgcagctggc
     1801 cgaccacgcg taccgctacg tgcggatcaa gcccggcacc gacgtcgcgt tcttcaacgc
     1861 ggtgatgcac gagatcgtgc gccgcgggct cgtcgaccag agcttcatcc gcgagcgcac
     1921 ggagggctac gaggccctgc aggcgatggt gtcgcactac ccgccggagg tggcggcgcg
     1981 catctgcggg gtggagccgc aggagatcat cgacatcgcg gtcacctggg gctcggcgaa
     2041 cgcggcgatc tgcttctggg gcatgggcgt cagccagcac acccacggca ccgacaacgc
     2101 gcgatgtttg atctcgctgg tgttgatgac cgggcaggtc ggtcgcccgg gcaccggcct
     2161 gcacccgctg cgcgggcaaa acaacgtgca aggggccagc gacgccggcc tgatcccgat
     2221 ggtgttcccc gactaccagt cggtcaagga cccgacgatc cgcgcgcgct tcgaggcggc
     2281 gtggggcgtg ccgctcgacg cccagccagg gttgacggtc gtcgagatca ttcacgccgc
     2341 gctgcacggc gacgtgcgtg gcatgtacat ccagggcgag aacccgttca tcagcgaccc
     2401 gaacaccgcc aaggtccgca aggccctcgc ggcgctcgac ttcctggcgg tgcaggacat
     2461 ttttcttacg gagacggccg agttcgccga cgtgatcctg ccggcctcga gcttctttga
     2521 aaaaaccggc acgtacacga acaccgaccg gcgggtgcag atcggccggc ccgtgctcaa
     2581 gatgccgggc gaggcccggc tcgactggca gatcacctgc gagatcgcca ggcgcatggg
     2641 cgcgccgggc ttcgactatg aaagcgtcga tcaggtgttc gacgagttcg ccgcgctgac
     2701 cgacagctac cgcggtctgt cgtacgaaca cctcggcctg gaaggcaagc tctggccgtg
     2761 cccggatccg gcgagctccg acggcacggt cgtgttgttc acggaggact tcccgagcgg
     2821 ccgcggcaag atggtccccg cggagttcgc cccgcccgcc gagctgccga accccgagtt
     2881 cccgatcgtg ctcacgaccg ggcgcctgct cgagcactgg cacaccggca cgatgacgcg
     2941 cagggccgtc gcgctcgacg cgatcgaggg cgagccgcac gtcgacatgc acccggagga
     3001 catgcgcacg ctcggcctcg ccgacgagga ctgggcgacg gtgacgagcc gccgcgggag
     3061 catcacgctc aaggtgcgag gcagccgctc gccgggccgc ggcgcggtgt tcatcccgtt
     3121 ctgctggcgc gaggcggccg ccaacgtgtt gacgatcgac gagctcgacc cgtacgggaa
     3181 aattcccgag ttcaagttct gcgccgtgcg cgtcgagcgg gccagcccgg gcgacatgca
     3241 cgacctcgac gagcccgaga cggaccgcag cgcggccgag tgacgatggt cgagatcttc
     3301 ctcgtctcga tggtcgcgtt gctcggcggc ttcggcttct accgcctggt gcgcgcgcag
     3361 caggaggccc ggcgctacct cgagagcggc accgacgcgc cgctgcagct gtcgcacctc
     3421 agcagcgacc ttgcaaaagt cgcccgcgag acgcggacgc tgaggatctc gctcgagagc
     3481 ccgctgcgtg cgatccgcga gttcatgggc accgacctga cgcgcaccga ggacgacgtc
     3541 gaggcggtcg acgccgcgct gatggaggcg acccgcgaca tcggcgtgtg gctggcgctg
     3601 gtggattcgc tgcccgagag cgaacgcggc acgctcgagg cgatgggcgc gagcccgggc
     3661 gccgtgcggg cggccctcgc cgccgagggc tgggccttcg agcgcagcca cctgcgtgcc
     3721 ccgggtcgcg gcaacctgga cgatcgactc gaggcgatcc tgcgagagct cgagcgcgtc
     3781 gagaaggcgc tgcaggtgcc gatcaacccc tatcgctagg cgaatgagtc gcgtcgccgc
     3841 ggtctgtgcc aggccgatgc acgcaggtac gtgcggctct gcacttgcgg atgtggcatg
     3901 cgcctgtcag ggtgcactga tgcgcttact gacttgtgta ttatcgctgg cgatcctcgg
     3961 gatcgcagcg cgggcggagg cgacgacctg cggcgatcgg accgcgccgt ggtgggaccc
     4021 gtacgcgact ccgctgtact ccgaggacca gccgctcccg atcgacgggc agccgtggca
     4081 gttcgtcgac tgctcgtccg agggggtcgc gatcggcgcg tgcgagttcg tcgatgcgga
     4141 agggctgcgg acccccgtcg aggtggagga cgtcggggtc gagctgtgcg agctgcgcta
     4201 cggtgcgggc gtccgcgaga cggcctcctc gttcattcgc cgcttcgtgc cgccggcgcc
     4261 gctggtcccg ggcgcgtaca cgcttgagtg cgagggcagg gggtccggct cgttcgagat
     4321 ccgcgagacg gacaccccgg ccgcgccgcc gaccccgctc gtgttcaccg acacccactt
     4381 gacccgcggg gacgacggtg gatgctgcgg cgggttcggc gatgtcgtcg agctgcgcgt
     4441 cgccgacgac ctgccgtacc tgcacgaggg cggctacgtc gaggtgaagt atccgaacgg
     4501 gcaacacctc gcgctgacgc gcgccgagga cgggcagttc gtgttgccgt gggaccaggt
     4561 cgtcatcgaa ctgacgccgg tgtccgccag cggcgcgcgc ggcgagacgg tcgtgctcga
     4621 cctcgccgag gtcgacggcg acctcgcgta cgtgccgtgt gatgtcgctg gccggcgccc
     4681 gtcggccgcg ctgtggctgc tcgcgccgct cgcgtggctg tacacgcagg gccggcgccg
     4741 gcggaggtgc gcatgagccc cgtcgtgctt ctgtgggtgt ctctcgggac aggttcggtg
     4801 caggcgcccg acgggccgcc gaccgccgcg caggacagcg cggcgccggc cagcatgccg
     4861 tcaccgcccg ccccggcgcc cgctccggcg gcgagccccg gcgtcgtcgc cgtcgcatac
     4921 accccgtacc agccgcagcc gcagcgcgcc gtgccgccgc ggcgcttcgt ggtcgcgttc
     4981 ttgccgagcc tgacgatggg catcagcccg cggccctcgt tcaacctcgc gggcttcgtc
     5041 ggcgggcggc tcccgggcgc gtgggcgctc ggttatcagt tcgccttctc ctccggcttc
     5101 gccgagcgct acagccgggg cctgctgacg caccgccatc acgtcatggc gatgcgggcc
     5161 ttcggagcga gcggccgcgg gttcgccagc gtcggcgcag gggcggcctt cctcgagctc
     5221 aatccggtgg tcgaggccga gggtcgcctg ggcgtccgct tcggccgcaa gaagtacggg
     5281 gtcgtcgcag cgatgatgcg cctcggctgg gacatcggcc accgcgagcg ggtcccgatg
     5341 ccacagttcg ggatcgtgtt cggcttcgcg gtgatgtgac cggccggcga cctgcgcttt
     5401 tggacaggtc gccgcgagcc gccgatctcc ggcgcgacct gtcccttagc gcaggtcgcc
     5461 gagcaccgtc agcagcgcgt cgaggttggc gcggccgagc tggcggcagc gctcggggga
     5521 ccagccgacg gcctcgttcg gggtgtcttt ggtgtccttg aacggcatct cgagggtcat
     5581 cgccaggcag ccgaagtggc cggcgacgtg ggtggtgcac atcgtcatgt tggcctggcc
     5641 gggcggcacg gggtcgtagc cgtgctccat ctggaagtcg gggctggcgc gcatcagggc
     5701 cttctcgtag ctgacctgca gggcgcgcac gcgctgcgtc accgaggcga cgccgtcggc
     5761 cccggcgatg aagttgtagg gcagggcctc gtcgccgtgc acgtcgaggc agaagtcgac
     5821 gccggtcgcc tccatgcgcc ggcggaccag caagatctcc gggctgcgct cggcggtcgg
     5881 gttctgccac tcgcggttga ggttggcccc ggccgcgttg acgcgcaggt tgccgcgaaa
     5941 gctgccgtcg gggttcatgt tgggcaccag gaagaacacg gcgcggctgc gcagcgtgcg
     6001 ggcgacgggg tcctcggggt cgatcaggtg ggcgatcagg ccctccatca accactcggc
     6061 catcgtctcg ccagggtgct ggcgggcgac gatccagcac acgcggcgat cgccgccgag
     6121 ctccccgatc cgcagcaggt ccatgtcgcg gccctcgagg gtgtggccga gcagctcgtg
     6181 gcgcacgccg ggcagcgcga gcgagcgggc gatcaggtcc tggtggcgct cgtgcgagta
     6241 gggcgcaaag tagctgtagt agacgacgtc acgcgtcggg gtgtggcgga tcgtcaggcg
     6301 cttgccgtcg tagtcggtcg gcacacggaa gaagtgctgg cgatcgtagg tggcgacggc
     6361 gcggtagtcc tgccagcccg gcgggtacga ggtctcgccc gcgttgtcga tgtgcaggtt
     6421 gagcgcgacg ccctgggcct cgctgacgcg gaagttgaac cactgcaaaa actcgctgtt
     6481 gctgtcgtgg cggatccgca ggtgcgcgtc gtcgggtcgg gtgatgtcga cgacctcgat
     6541 gttcccgccg tcgaactggc tgctgacctt catcgcgcga tgctagccca tgggcgcgcg
     6601 cagcaccaag aagccgccac gaccccgaac gtggaaacgc cgtc
//
LOCUS       DASWNJ010000981         5061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_18474, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000981 DASWNJ010000000
VERSION     DASWNJ010000981.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18474"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2336)
                     /locus_tag="VGB85_16655"
     CDS             complement(<1..2336)
                     /locus_tag="VGB85_16655"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEY0135718.1"
                     /translation="MQLLVTPLWVWLATAPGAPSTPAAPSTPGTTAPARSSTAATPTQ
                     TGAVPAGAVPRPGPIDMTGAPEGVKKVAAPVEPPKAIIGRERIEQLRLPAHRIDDEAI
                     TIDGHVTESAWNLTPQAPELVQFEPRFGFGPTQETDVRIAYGRKGIYVAFRLKGDPKK
                     VRGGVFRKDQIGPSDLVWLIIDPTNNDTNGYVFAVNPSGSQHDAQLFRDDSEEPLWDG
                     VWQTAAAITTTGWSAEIFVPFSTIRFDAREEHVFGVNIGRYINDNGETDVLSPTPQGK
                     PGVISWALDYYGIKGVEPGLNLELRPFTSFRIAARRPPDSFDRSIPFLPNGGFDVKYG
                     LRGNLTLDLAVNPDFGQAEVDPAVLNLGPFEVFFPERRTFFLESKEIFETRFQLFYSR
                     RVGNNPRPARADLTTRTDRDGKPETGQLYALDPLTRIFGALRITGQAARSWSVGALTA
                     TTGATYGSERFSDGTEHRVTVDPTSQYSVFRVRKEFDSQTSVGAIATGVVRGGGEPSA
                     FTGGFDYRILFAERWRHSAQVIATHDGHRTGMGAGFDLRRSGKNMSLGTSVDMLTPHA
                     NFNDLGFMRLNNFIDGSANVSVYNAQPVGRLRRLAGGLATRVASSFQGQLLQKQLTGD
                     IGLTTLNLWQFGFNTGGHLPQLDLYETRGGMPYEVPLHWWTGLDVTSPDNRRVVAAFN
                     GAYGEQNGYPGPDIALELRIRPVNRLELTLRGDFNATYDRPRWTTTNALDEAVFARAR
                     VNSYTGLLRGTLGILPTLTLQSFNQLYYLSAHHTDFFIL"
     gene            2475..3644
                     /locus_tag="VGB85_16660"
     CDS             2475..3644
                     /locus_tag="VGB85_16660"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEY0135719.1"
                     /translation="MAYKLVTLGGDGTGPEVMREGLRVLHAISGALDLAWDVDDIACG
                     GQYYLEHGDRDWPVGSEERCDAADLILLGAVGWPSPTGSGPVLMKDGKMAGFSPVIGN
                     RTRLNLYANVRPVKLYDGVQHRIHGANKLVWQPGQVDMVMIRENTEGLYTPTGGKLAP
                     GGRTDVAIDTRVITRRGSEQVIRHAFELCRRRNKGAPKDGKLRVTQLVKDNVLHGCQL
                     FSQVFYEVGEEYPEIEKDSAIIDAFTQWLIGQPEYYDVVVTTNMFGDIVTDLASVLQG
                     GMGMAVGANVGKGHGMFEPIHGSAPKHAGKDKVNPIAMILAVKEALEWLGGEKNDERL
                     GRGARAIEAAVIELLKLGAPLTYDLVGEAKAAKASEVGQWLAEAAVRHLGAQAQA"
     gene            complement(3717..>5061)
                     /locus_tag="VGB85_16665"
     CDS             complement(3717..>5061)
                     /locus_tag="VGB85_16665"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY0135720.1"
                     /translation="AEQQQSTLTSLSDRLEGRPGRDAVLALWNRHARLPPVFRVDRTL
                     EAVRNDPNVPPATRAAAETVLRASRGTARDVSDDYIELMTDGSVIRAAIAEHGQGDFS
                     DTDIASIVGWCAAKHELDAEVPAARGGDREEPEDDSSSRGVPDLDHEPRPPKGERDDD
                     ESPDAEPARPRHLPAHVGLDAEDDAILLRLIQLKYGGIFVGGKRFEYEHIVIDEAQDL
                     APLEVRVLLDCVTEGQSVTISGDRAQKMIFDNGFTDWPELLRDAGLPHVAIQPLKISY
                     RSTRQVMELSRHVLGPLHNADEQLIAREGAPVGFYAFGDTGEAVAFLAEALRNLMQRE
                     PTASVALIARYPQQADVYHDALRIAEVPKLRRVGRQDFSFTAGIDVTDVRQVKGLEFD
                     YVVILDPTTQNYPEVVESRHLMHIAATRAAHQLWLVCTGPVSKLIPEDVIHASTE"
BASE COUNT          736 a         1578 c         1969 g          778 t
ORIGIN      
        1 aggatgaaga agtcggtgtg gtgggccgag aggtagtaga gctggttgaa ggactgcagc
       61 gtcagcgtcg gcaggatgcc gagcgtgccg cgcagcaggc cggtgtacga gttgacgcgg
      121 gcgcgcgcga acacggcctc gtcgagggcg ttggtggtcg tccagcgcgg gcggtcgtag
      181 gtcgcgttga agtcgccgcg cagggtcagc tcgaggcgat tgacgggccg gatcctcagc
      241 tcgagggcga tgtccgggcc ggggtagccg ttctgctcgc cgtaggcgcc gttgaaggcg
      301 gcgacgaccc ggcggttgtc cggcgaggtg acgtcgaggc cggtccacca gtgcagcggg
      361 acctcgtacg gcatgccgcc gcgtgtctcg tacaggtcca actgcggcag gtggccgccg
      421 gtgttgaagc cgaactgcca caggttgagg gtcgtcaggc cgatgtcgcc ggtcagctgc
      481 ttttgtagca gctggccctg gaagctcgag gcgacgcgcg tggcgaggcc gccggcgagg
      541 cggcgcaggc gaccgaccgg ctgcgcgttg tagacgctga cgttggcgct gccgtcgatg
      601 aaattgttga gccgcatgaa cccgaggtcg ttgaagttcg cgtgcggggt cagcatgtcg
      661 acggatgtcc cgagggacat gttcttgccg ctgcggcgca ggtcgaagcc ggcgcccatg
      721 ccggtgcggt ggccgtcatg ggtggcgatg acctgggcgg agtggcgcca gcgctcggcg
      781 aagaggatgc ggtaatcgaa gccgccggtg aaggcgctgg gctcaccgcc gccgcgcacg
      841 acgcccgtgg cgatcgcgcc gacgctggtc tggctgtcga actccttgcg cacgcggaac
      901 acggagtact ggctggtcgg gtcgacggtg acgcggtgct cggtgccgtc gctgaagcgc
      961 tcggagccgt aggtggcgcc ggtggtggcg gtcagggccc cgacgctcca cgaccgggcg
     1021 gcctggccgg tgatccgcag cgcgccgaag atgcgggtca gcgggtcgag cgcgtagagc
     1081 tggccggtct cgggcttgcc gtcgcggtcg gtgcgggtcg tcaggtcggc gcgcgcaggt
     1141 ctcgggttgt tgccgacgcg acgcgagtag aacagctgga accgggtctc gaagatctcc
     1201 ttcgactcga ggaagaaggt gcggcgctcg gggaagaaca cctcgaaggg cccgaggttg
     1261 agcaccgcgg ggtcgacctc ggcctgaccg aagtcggggt tgaccgcgag gtcgagggtg
     1321 aggttgccgc gcaggccgta cttcacgtcg aagccgccat ttggcaggaa ggggatcgac
     1381 ctgtcgaagc tgtccggggg gcgacgtgct gcgatgcgaa acgaggtgaa gggccgcagc
     1441 tcgaggttga ggccgggctc gacacctttg atcccgtagt agtcgagcgc ccacgagatc
     1501 acgccaggct tgccctgcgg cgtcggcgag agcacgtcgg tctcgccgtt gtcgttgatg
     1561 taacgcccga tgttgacgcc gaagacgtgc tcctcgcggg cgtcgaagcg gatcgtcgag
     1621 aagggcacga agatctcggc gctccagccg gtggtcgtga tcgcggcggc ggtctgccac
     1681 acgccgtccc acagcggctc ctcgctgtcg tcgcggaaca gctgggcgtc gtgctgcgag
     1741 ccggaggggt tgaccgcgaa gacgtagccg ttggtgtcgt tgttggtcgg gtcgatgatc
     1801 agccagacga ggtcgctcgg gccgatctga tccttgcgga acacgccgcc gcggaccttt
     1861 tttgggtcac ccttgaggcg aaacgcgacg tagatgccct tgcgcccgta ggcgatccgc
     1921 acgtcggtct cctgggtcgg cccgaagccg aagcgcggct cgaactgcac gagctcgggg
     1981 gcctgcggcg tcaggttcca cgcggactcg gtcacgtgac cgtcgatcgt gatcgcctcg
     2041 tcgtcgatgc ggtgcgccgg cagccggagc tgctcgatgc gctcgcggcc gatgatcgcc
     2101 ttcggaggct cgaccggcgc ggcgaccttt ttcaccccct ccggcgcgcc ggtcatgtcg
     2161 atcgggcccg gccgcggtac ggcgcccgcc ggcacggcgc ccgtctgcgt cggtgtggcg
     2221 gcggtcgagg acctcgccgg ggccgtggtg ccgggggtgc tgggcgcagc cggggtggac
     2281 ggcgctcccg gagccgtggc cagccacacc cataacggcg tcacgaggag ctgcacgaga
     2341 gccgttcatg tataacccga gacggccgga gaaggggtga gcttccgcac cataaaactg
     2401 cgttctgggg cgcacagcgg ggtgctacgg ccgtgtacac cgagatcagg cccgcagggc
     2461 cggggagaca aggaatggcg tacaagctgg tgaccctggg tggcgacgga acgggcccgg
     2521 aagtgatgcg cgaggggctt cgggtgctac acgcgatctc cggcgcgctc gatctggcgt
     2581 gggacgtcga cgacatcgcg tgtggcggtc agtactacct cgagcacggc gaccgcgact
     2641 ggccggtcgg ctcggaggag cgctgcgacg cggccgacct gatcctgctg ggcgcggtcg
     2701 gctggccgtc gccgacgggc tcgggcccgg tgctgatgaa ggacggcaag atggcaggct
     2761 tctcgccggt catcggcaac cgcacgcggc tcaacctgta cgccaacgtg cgcccggtga
     2821 agctgtacga cggcgtgcag caccggatcc acggcgccaa caagctggtg tggcagccgg
     2881 gccaggtcga catggtgatg atccgcgaga acaccgaggg cctgtacacg ccgaccggcg
     2941 gcaagctggc gccgggtggc cgcacggacg tcgcgatcga cacgcgcgtg atcacccgcc
     3001 gcggcagcga gcaggtgatc cgccacgcct tcgagctgtg ccggcgccgc aacaagggcg
     3061 cgcccaagga cgggaaactc cgcgtcacgc agctggtcaa ggacaatgta cttcacggct
     3121 gtcagctgtt ctcgcaggtc ttttatgagg tcggcgagga gtacccggag atcgagaagg
     3181 actcggcgat catcgacgcg ttcacgcagt ggctgatcgg ccagcccgag tactacgacg
     3241 tcgtcgtcac gaccaacatg ttcggcgaca tcgtcaccga cctcgcgtcg gtgctgcagg
     3301 gcggcatggg catggcggtc ggcgccaacg tcggcaaggg gcacggcatg ttcgagccga
     3361 tccacggcag cgcgcccaag cacgccggca aggacaaggt caacccgatc gccatgatcc
     3421 tggccgtcaa ggaggccctc gagtggctcg gcggcgagaa gaacgacgag cgcctcggcc
     3481 ggggcgcccg cgcgatcgaa gccgccgtca tcgagctgct caagctcggc gcgccgctca
     3541 cctacgacct cgtcggcgag gcgaaggcgg ccaaggcctc cgaggtcggc cagtggctcg
     3601 ccgaggccgc ggtgcggcac ctcggcgcgc aagcgcaggc gtgaagtgag ccggcctgtc
     3661 ccccgggaca ggccgtggtg cgaccggcct gtccgttcgg acaggccggg gcgcggtcac
     3721 tccgtgctgg cgtggatcac gtcctcgggg atcagcttgc tgaccgggcc ggtgcacacg
     3781 agccacagct gatgcgcggc gcgggtggcc gcgatgtgca tcaggtggcg cgactcgacc
     3841 acctcggggt agttctgcgt ggtcgggtcg aggatgacga cgtagtcgaa ctcgaggccc
     3901 ttgacctggc ggacgtcggt gacatcgatg ccggcggtga agctgaagtc ctggcgaccg
     3961 acgcgacgca gcttgggcac ctcggcgatg cgcagggcgt cgtgatacac gtcggcctgc
     4021 tgggggtagc gggcgatcag ggcgacgctg gcggtcggct cgcgctgcat caggttgcgc
     4081 agggcctcgg cgaggaaggc gacggcctcg ccggtgtcgc cgaacgcgta gaagccgacc
     4141 ggggcgccct cgcgggcgat cagctgctcg tcggcgttgt gcagcgggcc gagcacgtgg
     4201 cgtgacagct ccatcacctg ccgcgtcgag cggtagctga tcttgagcgg ctggatggcg
     4261 acgtgcggga ggccggcgtc gcgtagcagc tcgggccagt cggtgaagcc attgtcgaag
     4321 atcatctttt gcgcgcggtc gcccgagatc gtgacgctct ggccctcggt gacgcagtcg
     4381 agcagcacgc ggacctcgag cggcgcgagg tcctgggcct cgtcgatgac gatgtgctcg
     4441 tactcgaagc gcttgccgcc gacgaagatg ccgccgtact tgagctggat gaggcgcagc
     4501 aggatcgcgt cgtcctcggc gtcgaggccg acgtgggccg gcaggtggcg cgggcgcgcg
     4561 ggctcggcgt cgggggactc gtcgtcgtcg cgctcgccct tcggggggcg cggctcgtgg
     4621 tcgaggtcgg gcacgccgcg gctgctgctg tcgtcctcgg gctcctcacg gtcgccgccg
     4681 cgggccgcgg ggacctcggc gtcgagctcg tgcttggccg cgcaccagcc gacgatcgac
     4741 gcgatgtcgg tgtcgctgaa gtcgccctgc ccgtgctcgg cgatcgcggc gcggatgacc
     4801 gagccgtcgg tcatcagctc gatgtagtcg tcggagacgt cgcgggcggt gccccgggag
     4861 gcgcgcagga cggtctcggc ggcggcgcgg gtcgccggcg ggacgttggg gtcgttgcgc
     4921 acggcctcga gggtgcggtc gacgcggaac accggcggca ggcgggcgtg gcggttccac
     4981 agggcgagca cggcgtcgcg gccggggcgg ccctcgaggc ggtcgctcag cgaggtcagg
     5041 gtgctctgct gctgctcggc g
//
LOCUS       DASWNJ010000982         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_548882, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000982 DASWNJ010000000
VERSION     DASWNJ010000982.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548882"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..992
                     /locus_tag="VGB85_16670"
     CDS             285..992
                     /locus_tag="VGB85_16670"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEY0135721.1"
                     /translation="MSRLRGLLFDLDGVLIDSLPSITACMNHALAQLGRPPLDPALIR
                     PLIGPPLEDSAALLLGTSEPAQVAAFVAVYRERYAATCVAETLPAAGLREILKELSQR
                     WPLALATSKPEVFARLLLDSFEVGACFQRGGIYGRSLALDNEDKAAVIARALVMLGGA
                     DGLVMIGDRKHDVIGAAAHGIPTIGMLHGMGSREELEQAGARWICDDLWGLPALLERI
                     DGDDEGDEGDEGDEDLT"
     gene            complement(996..>2008)
                     /locus_tag="VGB85_16675"
     CDS             complement(996..>2008)
                     /locus_tag="VGB85_16675"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135722.1"
                     /translation="TDQRVLKQQLLAREHGLQVDQVVAAPQEYGYRWSSKRVVGGTPG
                     QLVLGSYVRGSHTLAAMHACQVDHPEIVLAAEELRRVADSLAIEPHDERTGAGDLRYA
                     WFKTDGREVLLTLVTASDDSRAARELPERLRRPTGISWSIQPSHGNSLRGQPAQVLRG
                     RPELALELAGVPLTTGPLGFLQPNPEVAALAYADLVRAPDGAELSGPLALDLYAGAGV
                     TTQLLRRHFTEVRPCEAYPESAAALGVPPETAESFLKNQRDTPTAVIANPPREGLGPQ
                     VCAELVRLAPPRLNIMSCGPAGLARDLAALAPKFRLVALRAYDTLPHTPHVELVAWLE
                     AT"
BASE COUNT          278 a          602 c          831 g          297 t
ORIGIN      
        1 cttcgcgtga cctacacgca cgcgaggtca tgcaggtcac gagcacgacg aacgcgccgc
       61 gtcctcgtcg ccggtgctgt gaagtggagg tcacgacgac gcagacgacg cagcgatcgc
      121 agcgcgaggg gtcgcaggtg cgaagatata cggctcctgc gggccgcaaa caatcgtgtg
      181 catccgcacg cgatcgtgac aggtcggcgg agcctcgcct gtgccttcgg acaggcgcga
      241 tgcgggcgag gacgcgccgt gatcctgcgt gctagaccgc ggtcatgtcc cgtctgcgcg
      301 gtctcctctt cgatctcgac ggcgtgctga tcgactcgct gccgtcgatc acggcgtgca
      361 tgaaccacgc gctcgcacag ctgggccggc ccccgctgga ccctgcgctg atccggccgt
      421 tgatcggccc cccgctcgag gacagcgcgg cgctgttgct cgggacgtcg gagccggcgc
      481 aggtcgccgc gttcgtggcg gtataccgcg agcgctacgc ggcgacgtgt gtcgcggaga
      541 cgctgccggc ggctgggttg cgcgagattt taaaagaact gtcgcagcgc tggccgctgg
      601 cgctggcgac cagcaagccg gaggtgttcg ccaggctgtt gctggattcg ttcgaggtcg
      661 gcgcgtgctt ccagcgcggc gggatctacg ggcgctcgct ggcgctggac aacgaggaca
      721 aggcggccgt catcgcgcgg gcgctggtga tgctcggcgg ggccgatggg ctggtgatga
      781 tcggcgaccg caagcacgac gtcatcggcg ccgcggccca cgggatcccg acgatcggga
      841 tgctgcacgg gatgggctcg cgcgaggagc tcgagcaggc gggagcgcgg tggatctgcg
      901 acgacctgtg ggggctgccg gcgctgctcg agcgcattga tggcgatgat gaaggagatg
      961 aaggagatga aggagatgag gacctgactt gaaggtcagg tcgcttcgag ccaggcgacg
     1021 agctcgacgt ggggcgtgtg cgggagggtg tcgtaggcgc gcagggcgac gaggcggaat
     1081 ttcggggcga gggccgcgag gtcgcgggcg aggccggccg ggccgcagct catgatgttc
     1141 aggcgcggcg gggccaggcg caccagctcg gcgcagacct gcgggccgag gccctcgcgc
     1201 ggcgggttgg cgatcacggc tgtcggggtg tcgcgctggt ttttcaaaaa cgactcggcg
     1261 gtctccgggg ggacgccgag ggcggcggcg gactcggggt aggcctcgca ggggcggacc
     1321 tcggtgaagt ggcggcggag cagttgtgtg gtgacgccgg cgccggcgta gaggtcgagc
     1381 gcgagcgggc cggagagctc ggcgccgtcg ggggcgcgca cgaggtcggc gtaggcgagg
     1441 gcggcgacct cggggtttgg ttgcaggaag ccgagcgggc cggtggtcag cgggacgccg
     1501 gcgagctcga gcgcgagctc ggggcggccg cggagcacct gcgcgggctg gccgcggagg
     1561 ctgttgccgt gcgagggctg gatcgaccag ctgatgccgg tcggtcggcg caggcgctcg
     1621 ggcagctcgc gggcggcgcg gctgtcgtcg ctggcggtga cgagggtcag caggacctcg
     1681 cggccgtcgg tcttgaacca tgcgtagcgc aggtcaccgg cgccggtacg ctcgtcgtgg
     1741 ggctcgatgg cgagggagtc ggcgacgcgg cgaagttcct cggcggcgag cacgatctcc
     1801 gggtggtcga cctggcaggc gtgcatcgcc gcgagggtgt ggctgccgcg gacgtagctg
     1861 ccgagcacga gctggccggg cgtaccaccg acgacgcgct tgctcgacca gcggtagccg
     1921 tactcctgcg gggcggcgac gacctggtcg acctgcaggc cgtgctcgcg ggccagcagc
     1981 tgttgtttga gcacacgctg gtcggtga
//
LOCUS       DASWNJ010000983         3973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_548910, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000983 DASWNJ010000000
VERSION     DASWNJ010000983.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548910"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VGB85_16680"
     CDS             complement(<1..410)
                     /locus_tag="VGB85_16680"
                     /inference="COORDINATES: protein motif:HMM:NF024489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135723.1"
                     /translation="MPPVAPIDLTHPSLYINRELSQLEFNRRVLEQAKDKSLPLLERL
                     RFLTIFSTNLDEFYEIRVASIKEQVGFGLPQVSADGRSPQDTLREIKGVAQRLVKEQY
                     QILQEQILPALSAEGIRLLKLYTWTPDQSDWLEA"
     gene            complement(478..954)
                     /locus_tag="VGB85_16685"
     CDS             complement(478..954)
                     /locus_tag="VGB85_16685"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY0135724.1"
                     /translation="MQLFLVRHAIAVASAPDHPDATRPLTEKGRAKFVKAVRGAKALG
                     WSIDHLYHSPLTRAVQTAELLRPLLDGPSVALPDLARAPDEEMLAHIEGARVALVGHE
                     PHLGALLAWLVTGDVACGERFCFKKGGVAVLTGDPRPGAMTLQGLLAPKYLRRLGA"
     gene            1126..3462
                     /locus_tag="VGB85_16690"
     CDS             1126..3462
                     /locus_tag="VGB85_16690"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEY0135725.1"
                     /translation="MATANEATKPRWERAVEAIFRGMWEWGVLYALIAVSLGLALWPL
                     TNIEAMKYIVRNDLTVPQRSQALLAVAISVVVTTAGYLLVWLRGRRREPGLGLADSFR
                     RTNYYAFILLALPLVTALGVQKIEVKFPIFTAFLTVTITALAMVFGYRVLRHLFAPDA
                     EPFQPARRPRTALAFFIAAVLGYCGLVGYYAVIDHHNLGTAIYDLGIYDNAVWQTAHG
                     YFLDCTFIKGGNHVSAHFDPIIGVVALIYRLYMHAETLLVLQTVWLASGAIPIYLLAR
                     RRLKNEWFGALLALVYLMSPGLHGINMFDFHSLALIVPNIMWAVYLLDTDGYKRYWLV
                     IVALLLTREDISLICCFIGAYAILSGRTRTGLATIAVSMAYLYVAKVHVMASTDLLMA
                     SDNSSYSYAYYYADMIPHKKEGTRGLIMTAITNPIYTGSLVLKEDKLLYFIHLLLPLL
                     CLPFAAGRKLILSVYGLLFIGLASRKHVFSLHFQYSALLLPMLFAAAPDGLGRVVDGP
                     RMKLLGLERARLAWTLMFGMLVATTITSVKYGVIFPNSSFKAGWNRLTRIPTAEMREK
                     YVRVKEIAELIGPDAAVSVTSAVGPHLSNRRKAYHWPTVNDADYLVLNSELKKDDERR
                     LKRLRDKKQFRLIDDVAGIALYQRIEEDDPDAAPATAVKPKPRRKPATETTATDAPEA
                     GGAPVGTPGSAVIGPALPPTTSTARPSTATSGAGAGSGPAGSAGSAASTRPATGAAPS
                     SVPSTAPGKTPGKAAGSGASATGAGGTVPKGPAGAASP"
     gene            3529..>3973
                     /locus_tag="VGB85_16695"
     CDS             3529..>3973
                     /locus_tag="VGB85_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135726.1"
                     /translation="MSTSMNQPKDPSRPRFLIATRFLALTLTASVSAASVTACGGGAG
                     TERWVATENTTVEIDWDAINQAYKDAEGPEDFEKRVNEIYTGDEIISVSVRDVDEKTQ
                     VVTGFFDTNEDGQVADAEKVFTIKRDIVDPEKAQYAISGHGAYGHY"
BASE COUNT          631 a         1399 c         1326 g          617 t
ORIGIN      
        1 taggcctcga gccagtcgga ctggtcgggc gtccaggtgt agagcttgag caggcggatg
       61 ccctcggccg acagcgccgg gaggatctgc tcctgaagga tctggtactg ctccttgacc
      121 agccgctgcg ccacgccctt gatctcgcgc agggtgtctt gcgggctgcg gccgtcggcg
      181 ctgacctgcg gcagcccgaa gccgacctgc tccttgatgc tggcgacccg gatctcgtag
      241 aactcgtcga ggttggtgct gaagatcgtc aggaagcgca gccgctcgag cagcggcagg
      301 ctcttgtcct tggcctgctc cagcaccctg cggttgaact cgagctggct cagctcgcgg
      361 ttgatgtaga ggctggggtg ggtgaggtcg atgggagcga cgggcggcat cgcggtcctt
      421 gcggcggcgg cagaggagag cagggcaggg aggctatcag cctggagggt catgcgatca
      481 ggcgccgagc cggcgcaggt acttcggcgc gagcagaccc tgcagggtca tcgcgcccgg
      541 gcgcggatcg cccgtcagca ccgccacgcc gccctttttg aagcaaaagc gctccccgca
      601 ggcgacgtca cccgtcacca gccaggccag cagcgcgccc aggtgcggct cgtgaccgac
      661 caatgcgacc cgcgcgccct cgatgtgcgc cagcatctcc tcgtccggcg cgcgcgcgag
      721 gtccggcaag gcgacgctcg ggccgtccag cagcggacgc agcagctcgg ccgtctgcac
      781 cgcgcgcgtc agcggggagt gatagaggtg gtcgatgctc cagccgagag ccttcgcccc
      841 gcggaccgcc ttcacgaact tcgcgcggcc cttctcggtg agcgggcggg tcgcgtcggg
      901 gtggtccggc gcggaagcga cggcgatggc atggcggacg agaaagagct gcatggggcg
      961 tgggccggaa ggccgtgagt gtgcaccatc ttcggacgcg caggctcccg gccgacccgg
     1021 gcctggcccc cggcggcggt cgcggtcgct cgcttgctgg cctgcagggc cgctgataca
     1081 ctcccgcctc attttgcggc ttatgcgcag cgcgaaagat tactgatggc gacggcaaac
     1141 gaagcgacga aaccccgctg ggagcgcgcc gtcgaggcga tatttcgcgg catgtgggag
     1201 tggggcgtgc tctacgccct cattgcggtg tccctcgggc tggcgctgtg gccgctgacc
     1261 aacatcgagg cgatgaagta catcgtccgg aacgacctga cggtgccgca gcgctcgcag
     1321 gcgttgctgg cggtcgcgat ctcggtggtc gtgacgacgg cgggttacct gctggtgtgg
     1381 ttacgcggtc gccggcgcga gccggggctc gggctcgcgg actcattccg ccggaccaac
     1441 tactacgcct tcatcttgct ggcgctgccg ctggtgaccg cgctcggggt ccagaagatc
     1501 gaggtcaagt tcccgatctt caccgccttc ctgacggtga cgatcaccgc gctggcgatg
     1561 gtgttcgggt accgggtgct caggcacctc ttcgcgcccg acgccgagcc gttccagccg
     1621 gcgcgacggc ccaggacggc gctggcgttc ttcatcgccg cggtgctcgg ctactgcggg
     1681 ctggtcggtt actacgcggt gatcgaccac cacaacctcg gcaccgcgat ctacgacctc
     1741 gggatctacg acaacgcggt ctggcagacg gcgcacgggt acttcctcga ctgcaccttc
     1801 atcaaaggcg gcaaccacgt ctcggcgcac ttcgacccga tcatcggcgt cgtcgcgctg
     1861 atctaccgcc tgtacatgca cgccgagacg ctgctggtgc tgcagacggt gtggctggcg
     1921 agcggcgcga tcccgatcta cctgctcgcg cggcgccgcc tgaagaacga gtggttcggc
     1981 gccctgctcg cgctcgtcta cctgatgtct cccggcttgc acgggatcaa catgttcgac
     2041 tttcacagcc tcgcgctgat cgtcccgaac atcatgtggg cggtgtacct gctcgacacc
     2101 gacggctaca agcgctactg gctggtgatc gtcgcgctgc tgctgacccg cgaggacatc
     2161 agcctgatct gctgcttcat cggcgcctac gcgatcctca gcggacgcac gcgcacaggc
     2221 ctggcgacga tcgccgtgtc gatggcctac ctctacgtcg ccaaggtcca cgtgatggcc
     2281 tcgaccgacc tgctgatggc ctcggacaac tcgtcctaca gctacgcgta ctactacgcg
     2341 gacatgatcc cgcacaaaaa ggagggcacg cgcggcctga tcatgacggc gatcacgaac
     2401 ccgatctaca ccgggtcgct cgtgctcaag gaggacaagc tcctctactt catccacctg
     2461 ctgctgccgc tgctgtgcct gcccttcgcc gccggccgca agctgatcct cagcgtctac
     2521 ggcctgctgt tcatcggcct cgccagccgc aagcacgtgt tctcgctgca cttccagtac
     2581 tcggcgctgc tgctgccgat gctgttcgca gcggccccgg acggcctcgg ccgcgtcgtc
     2641 gatggcccgc gcatgaagct gctcggcctc gagcgcgccc gcctcgcctg gaccctgatg
     2701 ttcggcatgc tcgtcgccac gacgatcacc agcgtcaagt acggggtcat cttccccaac
     2761 agctcgttca aggccggctg gaaccgcctg acccggatcc cgaccgcgga gatgcgcgag
     2821 aagtacgtgc gcgtgaagga gatcgccgag ctgatcggcc ccgacgccgc cgtctcggtc
     2881 accagcgccg tcggcccgca cctgagcaac cgtcgcaagg cctaccactg gccgaccgtc
     2941 aacgacgccg actacctcgt cctcaactcc gagctcaaaa aggacgacga gcgccgcctc
     3001 aagcgcctgc gcgacaaaaa gcagttccgg ctgatcgacg acgtcgccgg catcgccctc
     3061 taccagcgca tcgaggagga cgacccggac gccgcgcccg ccaccgcggt caagcccaag
     3121 ccgcgccgca agccggccac cgaaaccacg gcgaccgacg cccccgaggc cggcggcgcg
     3181 ccggtcggca cgcccggctc ggccgtgatc ggaccggccc tgccgccgac cacgagcacc
     3241 gcgcggccga gcacggccac ttcgggcgct ggcgcgggtt ctggcccggc cggcagcgcc
     3301 ggttcggccg cgagcacccg gccggcgacc ggcgctgcgc cgtcctcggt gccgtcgacg
     3361 gcgcccggca aaaccccggg caaggcggcg gggtccgggg cgagcgcgac gggtgcgggc
     3421 ggcacggtgc cgaaggggcc cgcgggcgcc gcctcgcctt gacgggcccg gactgcgcca
     3481 ttagctttgc gagaccgcga tcggtgcctg aaaatcggcg tcgaacgcgt gagcacgtcc
     3541 atgaaccagc ccaaagaccc ctcccgtcct cgttttctga tcgccacgcg cttcctcgcg
     3601 ctcacgctca ccgccagcgt atccgcggcc agcgtgaccg cctgcggggg tggagcgggc
     3661 acggagcgct gggtggcgac cgagaacacc accgtcgaga tcgactggga cgcgatcaac
     3721 caggcctaca aggatgcgga gggcccggag gacttcgaga agcgcgtcaa cgagatctac
     3781 accggcgacg agatcatcag cgtatcggtg cgtgacgtcg acgagaagac ccaggtcgtc
     3841 accggcttct tcgacaccaa cgaggacggc caggtcgccg acgccgagaa ggtcttcacg
     3901 atcaagcgcg acatcgtcga ccccgagaag gcccagtacg cgatcagcgg ccacggcgcc
     3961 tacggccact acc
//
LOCUS       DASWNJ010000984         4506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_510804, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000984 DASWNJ010000000
VERSION     DASWNJ010000984.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510804"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VGB85_16700"
     CDS             complement(<1..439)
                     /locus_tag="VGB85_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135727.1"
                     /translation="MVHFIHVLAALLLLAPTTAPVAPAPAPAPAASTGTIAGAVTIHK
                     DSLTGKPKADRSGVVVYLEVEADEALADRLLDTPRPVLAVRQVNKTFMPAVSAVLTGT
                     EVSFPNDDMIFHNVFSLSKARRFDLGLYKSGTTKSITLTRPGII"
     gene            complement(447..1916)
                     /locus_tag="VGB85_16705"
     CDS             complement(447..1916)
                     /locus_tag="VGB85_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bZIP transcription factor"
                     /protein_id="HEY0135728.1"
                     /translation="MASVVLPLTLWVAPAVVPAPAEPAPPLMPAEPAPAEPTPVPAPV
                     VAADPASERLDRLERRLKALERENRGLRDEVTMLREDHDEVSERVDRVMPLTGRVGGY
                     LDVGFFYGMGDGSGIRPDIGYQNFPEYEGVVPDSWVFMGDPLSTTINSRGEPADTGAS
                     RAISFDGVNSQGKSSFILNAFNLNLLAGIGKGVSIEGLIDFVPRARNVADPDTSRFAL
                     GDYIDVKLGYLRWQVPVKRLDFDLYAGKIDPPFGYEYRIQESPSRIGVTPSLICRYTC
                     GRPLGIKARFRFLPRRSLIFAMSVTNGGSFTENFGFANEIDTNHFKTVMGRLSYVIPV
                     GSGLEIGASGAVGAQDFQPKDLVIQKQYGFDLHLEAKGVDLTGEFVMGKVDGDTEAGG
                     VRCGIAPCLDFRGAYGLLGYRVLTWLMPYARVDWRKAVHQSGASFVYNSNTLRITPGL
                     RFEVGTNVIIKLEYSVNRELGRVPGIRNDVFTSSLVARM"
     gene            complement(1931..2314)
                     /locus_tag="VGB85_16710"
     CDS             complement(1931..2314)
                     /locus_tag="VGB85_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135729.1"
                     /translation="MSPVELTVGELHARAGLDSDDPGVMRLTLVGTADATVEAALNEL
                     LGRLHSDSQRARAREVAVDLRKLEFMNSSCFKSFITWIVAVRRLAEDQTYRIRFLANA
                     GLHWQKRSLHAISYFGGDLVSVENA"
     gene            complement(2311..2784)
                     /locus_tag="VGB85_16715"
     CDS             complement(2311..2784)
                     /locus_tag="VGB85_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135730.1"
                     /translation="MTFAPTTRIVSVVRRFVMSLYERILEDDEMSSQLALVTHELLEN
                     AVKYNIDEATALRVTMTPQSETMSEIVVTIRTRNRATAENIRIAERLITEVRDAEDPF
                     MFYQQLIAVSADEPQRSGLGLARIRAETEMTIDFTIDGDEIEIVAHAQIRPGGEP"
     gene            complement(2906..>4506)
                     /locus_tag="VGB85_16720"
     CDS             complement(2906..>4506)
                     /locus_tag="VGB85_16720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0135731.1"
                     /translation="LDFDRKLETREEDDWLVNALVIGLNIMGDELRRRGDALTEALDR
                     ALAANRAKSAFLANMSHELRTPLNAIIGYSELLHEECSAVLGPHQLADLDKVVRAGRH
                     LLTLIKDTLDLSKIEAGKVELVVEELLVDALIDEVVGTVRPLVIERDNRLIVERGAGL
                     GSISSDRTKLLQILLNLLGNAIKFTTLGTVRLAVHREDDTLSLAVQDTGIGIPRDKLD
                     HIFGAFSQADEETTRRFGGTGLGLAITRHFCDMLGGTISVTSEFGAGSTFSLRLPARV
                     PARATAAVPDEARQRAPARGPALVASDDACVVEGLFTLLTGAGVPVLPVADVTELLRL
                     SAHVRPQLIILDEGLGEAELLTALSALHEDPERAAIPRFVVGELPPNVLVNGTVPLSR
                     PLRGASVLAAVARHIGRPPSLGTLVLVLGDAARGELPRRAFEARGWQVHEVVCPEEAA
                     SSPWPSVDAIVLDLGLPWAGEVAAGLRSRASARGPVLIGLGDDGQPAAEVCTTVLPAN
                     GRGFGRVHEALALECAGRPPRAPR"
BASE COUNT          697 a         1522 c         1606 g          681 t
ORIGIN      
        1 cgatgatgcc ggggcgcgtc agcgtgatcg acttggtcgt gccgctctta taaagcccga
       61 ggtcgaagcg ccgcgccttc gacagcgaga acacgttgtg aaagatcatg tcgtcgttcg
      121 ggaagctgac ctcggtgccg gtcagcacgg cgctgaccgc cggcatgaag gtcttgttga
      181 cctgccgcac cgcgagcacc ggccgcggcg tatcgagcaa ccggtccgcg agcgcctcgt
      241 cggcttcaac ctcgagatag acgaccaccc cggagcgatc ggccttcggc ttgccggtca
      301 ggctgtcttt atgaattgtc accgcgcccg cgatcgtgcc cgtgctcgcc gccggggccg
      361 gcgccggggc cggcgcgacc ggagcggtcg tgggggccag gagcagcaag gcggcgagga
      421 cgtggatgaa gtggaccatg gtgctcctac atgcgcgcga cgagcgagct ggtgaagacg
      481 tcgttgcgga tccccgggac ccgaccgagc tcgcggttga cgctgtactc cagcttgata
      541 atcacattgg tgccgacctc gaagcgcagg ccaggggtga tgcgcagggt gttgctgttg
      601 tacacgaagc tcgcgccgct ctgatgcacg gcctttcgcc agtcgacgcg cgcgtagggc
      661 atcagccagg tgagcacgcg gtagccgagc aggccgtagg cgcccctgaa gtcgaggcac
      721 ggggcgatcc cgcaccgtac gccgccggcc tcggtgtcgc cgtcgacctt gcccatcacg
      781 aactcgccag tcaggtcgac gcccttcgcc tcgaggtgca ggtcgaagcc gtactgcttc
      841 tggatcacca ggtctttcgg ctggaagtcc tgcgccccga ccgcacccga ggcgccgatc
      901 tcgagccccg agccgaccgg gatcacgtaa gacagccgac ccatcacggt cttgaagtgg
      961 ttggtgtcga tctcgttggc gaagccgaag ttctcggtga agctgccgcc gttggtgacc
     1021 gacatggcga agatcaacga gcgcctgggc aagaaccgaa accgcgcctt gatccccagc
     1081 ggacgcccgc aggtgtagcg gcagatcagc gagggcgtga cgccgatccg gctcggcgac
     1141 tcctggatgc gatactcgta gccgaagggc gggtcgatct tgccggcgta gaggtcgaag
     1201 tcgaggcgct tgaccggcac ctgccagcgc agatacccga gcttgacgtc gatgtagtcg
     1261 ccgagcgcga agcgggacgt gtccgggtcg gcgacgttgc gagcgcgcgg cacgaagtcg
     1321 atcaggccct cgatcgagac gcccttgccg atgcccgcca gcaggttgag gttgaaggcg
     1381 ttgaggatga agctgctctt gccctggctg ttgacgccgt cgaagctgat cgcacgcgag
     1441 gcgccggtgt cggccggctc gccgcggctg ttgatcgtcg tcgacagcgg gtcgcccatg
     1501 aacacccacg agtccggcac gacgccctcg tactcgggga aattttgata gccgatgtcc
     1561 gggcggatgc ccgagccgtc gcccatgccg taaaaaaacc cgacgtcgag gtaaccaccg
     1621 acgcggccgg tcagcggcat cacgcggtcg acccgctccg agacctcgtc gtggtcctcg
     1681 cgcagcatcg tcacctcgtc gcgcagcccg cggttctccc gctcgagcgc cttgaggcgt
     1741 cgctcgaggc gatcgaggcg ctcactcgcc ggatcggcgg cgacgaccgg cgccggcacg
     1801 ggcgtcggct cggcgggcgc gggctcggcg ggcatcagcg gcggcgcagg ctcggccggc
     1861 gcaggcacga ccgccggagc gacccacagc gtcagcggca ggacgacgga cgccatgatc
     1921 tgtcagcctc tcaggcgttc tcgaccgaca cgaggtcgcc gccgaagtag ctgatcgcgt
     1981 ggaggctgcg cttttgccag tgcaggccgg cgttggcgag gaagcggatc cggtacgtct
     2041 ggtcctcggc gagccggcgc acggcgacga tccacgtgat gaacgacttg aagcacgacg
     2101 agttcatgaa ctcgagcttg cgcaggtcga cggcgacctc gcgcgcgcgg gcccgctgcg
     2161 agtcgctgtg caggcggccg agcagctcgt tgagcgcggc ctcgacggtg gcgtccgcgg
     2221 tgcctacgag ggtcaggcgc atcacccccg ggtcgtcgct gtcgagcccg gcgcgcgcgt
     2281 gcagctcgcc gacggtcagc tcgacggggc tcacggctca cctccggggc ggatctgggc
     2341 gtgggcgacg atctcgatct cgtcgccgtc gatcgtgaag tcgatcgtca tctcggtctc
     2401 ggcgcggatg cgggcgaggc cgaggccgga gcgctgcggc tcgtccgccg agaccgcgat
     2461 cagctgctgg tagaacatga atgggtcctc cgcgtcgcgt acttcagtga tcagccgctc
     2521 ggcgatgcgg atgttctccg cggtcgcccg attgcgggtc cggatcgtca cgacgatctc
     2581 cgacatcgtc tcgctctgcg gcgtcatcgt cacccgcagc gcggtcgcct cgtcgatgtt
     2641 gtacttcacc gcgttctcga gcagctcgtg ggtcaccagc gccagctgcg aggacatctc
     2701 gtcgtcctcg aggatccgct cgtagagcga catcacgaag cgacgcacga ccgacacgat
     2761 gcgggtcgtc ggcgcgaagg tcatgtcgaa agcggccggt aacgccatca atcccgccct
     2821 cgagccgctt ccaggcgcat gcaacatgcg cggccacagt aaccggtttg gctgcaggac
     2881 aggtaaaaac cggcgcgaag gttcgtcatc gcggggccct cggcggacga cccgcgcact
     2941 cgagcgcgag cgcctcgtgc acgcgtccga agccccgccc gttcgccgga agcacggtcg
     3001 tgcagacctc cgccgcgggc tgcccgtcgt cgccgagccc gatcaagacg ggtccgcggg
     3061 cgctcgcgcg cgaccgcaac ccggccgcga cctcgccggc ccacggcaac ccgaggtcga
     3121 gcacgatcgc gtcgaccgag ggccacggcg agctggccgc ctcctcggga cacacgacct
     3181 cgtgcacctg ccatccgcga gcctcgaagg cgcgccgcgg cagctcgccg cgcgcagcgt
     3241 cgccgagcac gaggaccagc gtgcccagcg acggtggccg gccgatgtga cgcgccaccg
     3301 cggccagcac ggacgcgccg cgcagcgggc gtgacagcgg gacggtgccg ttgacgagca
     3361 cgttcggcgg cagctcgccg acgacgaagc gggggatcgc cgcgcgctcg gggtcctcgt
     3421 gcagggccga cagggcggtg agcagctcgg cctcgccgag cccctcgtcg aggatgatca
     3481 gctgcgggcg cacgtgcgcg gacaggcgca gcagctcggt gacatcggcc accggcagga
     3541 ccggcacgcc ggcgccggtc agcagcgtga acagcccctc gacgacgcag gcgtcgtcgc
     3601 tggcgacgag cgccggaccg cgagccggcg cgcgttgcct ggcctcgtcg gggaccgccg
     3661 cggtcgctcg cgcgggcaca cgggcgggca ggcgcagcga aaaggtcgag ccggccccga
     3721 actcgctggt gacggagatc gttccgccga gcatgtcgca gaagtgacgg gtgatcgcca
     3781 gcccgaggcc ggtgccgccg aagcgccgcg tggtctcctc gtcggcctgg ctgaaggcgc
     3841 cgaagatgtg gtcgagcttg tcgcgcggga tgccgatgcc ggtgtcctgc acggcgaggc
     3901 tcagggtgtc gtcttcacgg tgcacggcga ggcggaccgt gccgagggtg gtgaacttga
     3961 tcgcgttgcc gagcaggttg agaaggatct gcagcagctt ggtgcggtcg gaggagatgc
     4021 tgccgagccc ggcgccgcgc tcgacgatca gccggttgtc gcgctcgatc accagaggcc
     4081 gcacggtgcc gacgacctcg tcgatcaggg cgtcgacgag cagctcctcg accaccagct
     4141 cgaccttgcc ggcctcgatc ttcgacaggt cgagggtgtc tttgattaaa gtcagcaggt
     4201 gccgcccggc ccgcacgacc ttgtcgaggt cggcgagctg atgcgggccg agcaccgcgc
     4261 tgcactcctc gtgcagcagc tcgctgtagc cgatgatcgc gttgagcggc gtgcgcagct
     4321 cgtggctcat gttggccagg aaggcgctct tggcccggtt ggccgccagc gcccggtcga
     4381 gcgcctcggt gagcgcgtcg ccgcgacggc gcagctcgtc gcccatgatg ttgaggccga
     4441 tcaccagggc gttgacgagc cagtcgtcct cctcgcgcgt ctcgagcttg cggtcgaagt
     4501 ccagcg
//
LOCUS       DASWNJ010000985         1824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_688597, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000985 DASWNJ010000000
VERSION     DASWNJ010000985.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688597"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="VGB85_16725"
     CDS             complement(<1..1284)
                     /locus_tag="VGB85_16725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135732.1"
                     /translation="MHRNFTLALPVTLLFAACTFKVGDLGELTEASATEDPSTSSATD
                     PVTTSAGTAISGQTNGESGNTSGSEETGSSDPTEGLTPPDGVDILFVIDNSGSMAQHQ
                     QRLSTAIPALVAPLEAAGLDLRIAVTTTDAGNPRCPNATYTPEGGAFQARSCREAIAD
                     NQFKFGEDDFASSCLDACPHETITFSPTSTDVDPSPAVRPWIEWSEGAGNVAVPLSEA
                     IGCVLPQGVAGCGFESPLEAMFLALSRAQDPADPAFGFLRTSADLLVVMVTDETDCSW
                     APAAKEIFTTNKVFWNDPVNDPAPTSALCWRAGIECSGGPGTYDDCVAVDKGLDGTVT
                     NDPSQAVLQPISRYQGVLSTILASKQQAGSAAKVHVAAIAGVPVGYPGNPLVFADGDD
                     AFVQGNYGIGPGCEGDGTIAVPPGRIREVVEQTAPL"
     gene            complement(1336..>1824)
                     /locus_tag="VGB85_16730"
     CDS             complement(1336..>1824)
                     /locus_tag="VGB85_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135733.1"
                     /translation="LWRLAPEAGQQAGERGGLAAIFQRVDPDGSRRAAARAPTVRPAP
                     QPVPGDMTPQTAPAPETPPESPVPRDRPAGDLARELAQVRAAGEALRDGRGEQALAAA
                     DAYLRAYPGGAFVPEARLHRSEALCLLGRGDDARAAAEAFLRDLPDSPLRARVRSVCA
                     EK"
BASE COUNT          226 a          668 c          668 g          262 t
ORIGIN      
        1 gagcggcgcc gtctgctcga cgacctcgcg gatgcgcccc ggcgggaccg cgatcgtccc
       61 gtcgccctcg cagccggggc cgatgccata gttgccctgc acgaaggcgt cgtcgccgtc
      121 cgcgaacacc agcgggttgc ccgggtagcc gaccggcacc ccggcgatcg ccgcgacgtg
      181 caccttggcg gccgagcccg cctgttgttt gctggcgagg atcgtcgaca gcacgccctg
      241 atagcgcgag atcggctgca acaccgcctg gctcgggtca ttggtgacag tgccgtcgag
      301 gcccttgtcg accgcgacac aatcgtcgta ggtgcccggc ccgccgctgc actcgatccc
      361 cgcgcgccag cacagcgcgc tggtcggggc ggggtcgttg accgggtcgt tccagaacac
      421 cttgttggtg gtgaagatct ccttggcggc cggcgcccac gagcagtcgg tctcgtcggt
      481 caccatcacg accagcaggt cggcgctcgt gcgcaggaac ccgaacgccg gatccgcggg
      541 gtcctgtgcg cgcgacagcg cgaggaacat cgcctcgagc gggctctcga agccgcagcc
      601 ggcgaccccc tgcggcagca cgcagccgat cgcctccgac agcgggacag cgacgttgcc
      661 ggccccctcg ctccactcga tccacgggcg caccgccggg ctcgggtcga catcggtgct
      721 cgtcggcgag aaggtgatcg tctcgtgcgg gcaggcgtcg aggcagctcg acgcgaagtc
      781 gtcctcgccg aacttgaact ggttgtcggc gatcgcctcg cggcagctgc gggcctggaa
      841 cgcgccacct tccggcgtgt aggtcgcgtt ggggcagcgc gggttgccgg cgtcggtcgt
      901 cgtcaccgcg atccgcaggt cgaggcccgc ggcctcgagc ggggcgacga gcgccgggat
      961 cgccgtgctc aggcgctgct ggtgctgcgc catcgagccc gagttgtcga tcacgaacag
     1021 gatgtcgacg ccgtccggcg gcgtcagccc ctccgtgggg tcgctcgagc cggtctcctc
     1081 ggagccgctg gtgttgccgg actcgccgtt ggtctgaccg gagatcgccg tgccggcgct
     1141 cgtcgtcacg gggtcggtcg cggagctcgt cgaggggtcc tcggtggcgc tggcctccgt
     1201 caactctccg aggtctccga ccttgaaggt acaggcggcg aagagaaggg tgaccgggag
     1261 cgcgagggta aagttacggt gcattgcggg gtcctttcag cgtggcagtg gccgcccccc
     1321 gcgagttccg tcgaactatt tttcagcgca caccgagcgc acccgcgccc gcaacggcga
     1381 gtccggcagg tcgcgcagga acgcctccgc ggccgcccgc gcgtcgtccc cgcgaccgag
     1441 cagacacagc gcctcgctgc ggtgcagccg cgcctccggc acgaaggcgc cgcccggata
     1501 ggcgcgcaga tacgcgtccg cggccgcgag cgcctgctcg ccccgcccgt cgcgcagcgc
     1561 ctcgccggcg gcccgcacct gcgccagctc gcgcgcgagg tcgccggccg gcctgtcccg
     1621 cgggacaggt gactccggcg gcgtctcggg cgcgggtgct gtctgtgggg tcatgtcccc
     1681 ggggacaggc tgcggcgcgg gtcgcacggt cggtgcccgg gccgcggcgc gccgcgagcc
     1741 gtcggggtcg acgcgctgaa agatcgccgc caggcccccg cgctcgccgg cctgctgccc
     1801 ggcctccggc gcgaggcgcc acag
//
LOCUS       DASWNJ010000986         2904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_301696, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000986 DASWNJ010000000
VERSION     DASWNJ010000986.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301696"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1206
                     /locus_tag="VGB85_16735"
     CDS             67..1206
                     /locus_tag="VGB85_16735"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY0135734.1"
                     /translation="MRDAADIEVNPAHCRVCGAALAGSLAYVSMLLGVRRSARNPNLC
                     NRCTAHMKDGESVEITALFADLSGFTGLTQSHGDEQTAEIVDAFLRGASRAIQRQDGV
                     VDKFIGDAVMAVFNAPIRREDHCRRAVAAAIDIQNMMAALTRRFGVELQVSIGVARGT
                     ARLGRVGSDDVSSFTAIGGAVNLASRLQGAARPGTIAVDSAALADVADVFPDARTQAT
                     TLKGFEAPVAVAALHASTAAAEALRAAPSLIDRGRTIGRAAALMAALGAPCAGFYLFS
                     PIVYALGFGAVFQSALFLTIDTFLDDGPARTVLSSLAILSAAITLAMVVRVRLKRRRA
                     RQTASPGERMQERRLLFAAGLALAVVGLEHWVHLVSYHKGMWAAH"
     gene            1325..2698
                     /locus_tag="VGB85_16740"
     CDS             1325..2698
                     /locus_tag="VGB85_16740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0135735.1"
                     /translation="MTIDPPDDGDEADQRQVLQRRVAVVCMTAAAVVAVIDFSVWHSS
                     GDATKLSIALCMAVLAALFGVSLVLQRLGRSRRSLWPLLAALPVSIAWIMVAGGAATI
                     IPTMIAGLAMMLLLPDTLQPGVVSPRRWMTLLLAAYLAGIGLRIYFRGLDFASSPAEL
                     AILVLVPGALIFVQWLLIQRIFRDMRAALRESEALRRAHEQRNRELVASQLALERASN
                     AKSQFLANMSHELRTPLNIILGYTEILEEDVAEAGPADRTWGQDLARIRSAGVHLLSL
                     ISDVLDLSKIEAGKTTMFVEPFSLAELAREVCDACQPLIQRRANHLHLELDPQADELH
                     TDRLKLRQILLNLLSNAAKFTERGHIILRTRAGAPDHVVIEVQDTGPGISEEAQGRIF
                     DAFEQADTSPTRSHGGTGLGLTLVRRFSRLLGGDVSLDSEPGRGSLFRVTVLRRPPVQ
                     AATDALG"
BASE COUNT          387 a         1115 c          962 g          440 t
ORIGIN      
        1 cgtcgtccgc ccgtcacgcg ccgcaggggg taacatcggg tcggctgcga ctcgttggga
       61 tcccggatgc gtgacgctgc ggacatcgaa gtgaacccgg cgcactgtcg ggtgtgcggg
      121 gcggcgctcg ctggttcgct cgcctacgtg tcgatgctgc tcggcgtgcg tcgcagcgcg
      181 cgcaacccga acctgtgcaa ccgctgcacc gcgcacatga aggacggcga gtcggtcgag
      241 atcaccgcgc tgttcgccga cctgagcggc ttcaccgggc tgacgcagtc ccacggcgac
      301 gagcagaccg ccgagatcgt cgacgccttc ctgcgcggcg cctcgcgggc gatccagcgg
      361 caggacgggg tggtcgacaa gttcatcggc gacgccgtga tggccgtttt caacgcgccg
      421 atccggcgtg aggaccactg ccggcgggcg gtcgccgccg cgatcgacat ccagaacatg
      481 atggccgcgc tgacgcgccg cttcggggtc gagctgcagg tcagcatcgg cgtcgcccgc
      541 ggcaccgccc gcctcggccg cgtcggctcc gacgacgtct cgagctttac ggcgatcggc
      601 ggcgcggtca acctcgcgtc gcgcctgcaa ggcgccgcca ggcccggcac gatcgccgtc
      661 gactccgccg cgctcgccga cgtcgccgac gtgttcccgg acgcgcgcac ccaggcgacc
      721 acgctcaagg gcttcgaggc ccccgtcgcg gtcgcggcgc tgcacgcctc cacggccgcc
      781 gccgaggcgc tgcgggccgc cccctcgctg atcgaccgcg gccgcacgat cggccgcgcc
      841 gccgcgctga tggccgccct cggcgccccg tgtgccggct tctacctgtt tagcccgatc
      901 gtctatgccc tcggcttcgg cgcggtgttc cagtcggcgc tgttcctgac gatcgatacc
      961 ttcctcgatg atggaccggc gcgcaccgtg ctctcctcgc tggcgatcct cagcgccgcg
     1021 atcaccctcg ccatggtcgt gcgcgtgcga ctcaaacgcc ggcgcgcccg gcagaccgcc
     1081 tcgcccggcg agcgcatgca ggagcgccgc ctgctgttcg ccgccggcct cgcgctcgcc
     1141 gtcgtcggcc tcgaacactg ggtgcacctc gtcagctacc acaaggggat gtgggcagcc
     1201 cactgagcga cccccacagc gcatgtaggt gcatcgacgc cgcccgcggg cgcagacccg
     1261 cgcaaacacg cccgtggcca cgaagcggcg cttcctccat cctggaggcc gctgacgcgc
     1321 ttccgtgacc atcgaccctc ccgatgacgg cgacgaggcc gaccagcggc aagtgctcca
     1381 gaggcgggtc gcggtcgtgt gcatgaccgc cgcggcggtc gtggcggtga tcgacttctc
     1441 ggtgtggcac agcagcggcg acgccacgaa gctcagcatc gccctgtgca tggccgtgct
     1501 ggcggcgctg ttcggcgtct cgctggtgct ccagcgcctc ggccgttcac gccgctcgct
     1561 gtggcccttg ctggccgcgt tgccggtctc gatcgcctgg atcatggtcg ccggcggcgc
     1621 ggcgacgatc atcccgacga tgatcgccgg cctggcgatg atgctgctgt tgcccgacac
     1681 cctgcagccg ggcgtcgtct cgccgcggcg ctggatgacg ctgctgctcg ccgcctacct
     1741 cgcgggcatc ggcctgcgga tctactttcg tggcctcgac ttcgccagct cgccggccga
     1801 gctcgcgatc ctggtgctcg tcccgggggc cctgatcttc gtgcaatggt tgctgatcca
     1861 gcgcatcttt cgcgacatgc gcgccgcgct gcgcgagagc gaggcgctgc gccgcgccca
     1921 cgagcaacgc aaccgcgagc tggtcgccag tcagctcgcg ctcgagcggg cgagcaacgc
     1981 caagagtcag ttcctcgcca acatgtcgca cgagctgcgg accccgctca acatcatcct
     2041 cggatacacc gagatcctcg aggaggatgt cgccgaggcc gggcccgcgg accgcacctg
     2101 gggccaggac ctcgccagga tccgctccgc tggcgtgcac ctgctgtcct tgatctcgga
     2161 cgtcctcgac ctctccaaga tcgaggccgg caagacgacc atgttcgtcg agcccttctc
     2221 gctggccgag ctcgcccgcg aggtctgcga cgcctgccag ccgctgatcc agcggcgagc
     2281 caaccacctc cacctcgagc tcgacccgca ggccgacgag ctgcacaccg accgcctcaa
     2341 gctgcgccag atcctgctca acctgctctc gaacgccgcg aagttcaccg agcgcgggca
     2401 catcatcctg cgcacccgcg ccggggcgcc cgaccacgtc gtgatcgagg tccaggacac
     2461 cggcccgggc atcagcgagg aggcccaggg ccgcatcttc gacgccttcg agcaggccga
     2521 cacctcgccg acgcgcagcc acggcggcac cggcctcggc ctgaccctcg tgcgtcgctt
     2581 ctcgcggctg ctcggcggcg acgtcagcct cgacagcgag cccggccgcg gctcactgtt
     2641 tcgcgtcacc gtcctgcgcc ggccgcccgt gcaggccgcg accgacgccc tcgggtagcg
     2701 acattcgtcg caggtccccg agcagccgct gcatcgcgag gtggtgccgg gtcagctcgc
     2761 gcacctccgc gcgcagccgt tcgctgtcgc tggtgctcat atcgacgtct caatcgtcga
     2821 gcagcccgcc ggcgagcgcg gcgagcgagc tgtcgaagct cggctcgcag atcgagaaca
     2881 gccccggccc gagcggcgcc gtct
//
LOCUS       DASWNJ010000987         1538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_614817, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000987 DASWNJ010000000
VERSION     DASWNJ010000987.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614817"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1538
                     /locus_tag="VGB85_16745"
     CDS             <1..>1538
                     /locus_tag="VGB85_16745"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY0135736.1"
                     /translation="AKVLMDLKLIRRDVGQLILAAAMTDDTIGWILLSVVAGLATAGS
                     VDLLSAAKSIGAALLFLGLAFTIGAPIVARIVSGVDKAFGGSGAQMSAVLVLALGTAA
                     LTHMMGIEAVLGAFAIGILVGQAPRFRKDVAHTLELVTASFLAPIFFASAGVKVNLER
                     LADPEVLTVGGLVLGIACIGKFVGAYLGSWATGLSHWERLSMGSGMNARGAMEIIVAT
                     VGLSLGILTIEMYSIIVMVAIVTSLMAPPMLRFCLGRVKMGANEAKRLETEAVNANSF
                     IRQIRRVMIVTRSAHSVDLPAQIVGYLSHEQPIECTAIYARPQAVHSSWWQVYGRRAR
                     RYAKLGDLALDRIKRPLRLLKGTRPELKLISDPNPAEQVLVEADRDYELIVLGENRRA
                     AGSETLLGGLIDEVVRKAPCPTLIVREPPLQDGAKPAPYRVWSPKKILVPTVGTEYSK
                     NAVELAAVLAASTQAELMIVHITRTEANDIDAAIPRELGDQIVAREAERGRKFGATIN
                     TTVL"
BASE COUNT          224 a          610 c          468 g          236 t
ORIGIN      
        1 tcgccaaggt cctgatggac ctcaagctga tccgccgcga cgtcggccag ctgatcctcg
       61 ccgccgcgat gaccgacgac acgatcggct ggatcttgct gtccgtcgtc gccggcctcg
      121 cgaccgccgg cagcgtcgac ctgctgtccg ccgccaagtc gatcggcgcc gccctgctgt
      181 tcctcggcct cgccttcacg atcggcgccc cgatcgtcgc ccgcatcgtc tccggcgtcg
      241 acaaggcgtt cggcggctcc ggggcccaga tgtccgccgt gctcgtgctc gccctcggca
      301 ccgccgccct cacccacatg atgggcatcg aggctgtgct cggcgccttc gcgatcggca
      361 tcctcgtcgg ccaggccccg cgctttcgca aggatgtcgc ccacaccctc gagctggtga
      421 ccgcgagctt cctcgccccg atctttttcg cctccgccgg cgtcaaggtc aacctcgagc
      481 gcctcgccga ccccgaggtc ctgacggtcg gcggcctcgt gctcggcatc gcgtgcatcg
      541 gcaagttcgt cggcgcctac ctcggctcgt gggcgaccgg cctgtcgcac tgggagcgcc
      601 tgtcgatggg ctccggcatg aacgcccgcg gcgcgatgga gatcatcgtc gccaccgtcg
      661 gcctcagcct cgggatcctt acgatcgaga tgtactcgat catcgtcatg gtcgcgatcg
      721 tcacctcgct gatggccccg ccgatgctgc gcttttgcct cggccgcgtg aagatgggcg
      781 ccaacgaggc caagcgcctc gagaccgagg ccgtcaacgc caacagcttc atccgccaga
      841 tccgccgcgt catgatcgtg acgcgcagcg cccactccgt cgacctcccg gcccagatcg
      901 tcggctacct cagccacgag cagccgatcg agtgcaccgc gatctacgcc cgcccgcagg
      961 cggtgcacag ctcgtggtgg caggtgtatg gccgacgcgc gcgccgctac gccaagctcg
     1021 gcgacctcgc gctcgaccgc atcaagcggc cgctgcgcct gctcaagggc acccgccccg
     1081 agctcaagtt gatctccgac cccaaccccg ccgagcaggt gctcgtcgag gccgaccgcg
     1141 actacgagct gatcgtcctc ggcgagaacc gccgcgccgc cggctccgag accttgctcg
     1201 gcggcctcat cgacgaggtc gtccgcaagg ccccgtgccc gaccctcatc gtccgcgagc
     1261 cgccgctgca ggacggcgcc aagccggcgc cgtaccgcgt gtggtcgccc aagaagatcc
     1321 tcgtgccgac cgtcggcacc gagtacagca agaacgccgt cgagctggcc gccgtgctcg
     1381 ccgccagcac ccaggccgag ctgatgatcg tccacatcac gcgcaccgaa gccaacgaca
     1441 tcgacgccgc gatcccgcgc gagctcggcg atcagatcgt cgcccgcgag gccgagcgcg
     1501 gccgcaagtt cggcgccacg atcaacacca ccgtcctc
//
LOCUS       DASWNJ010000988         3362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_158253, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000988 DASWNJ010000000
VERSION     DASWNJ010000988.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158253"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(278..1594)
                     /locus_tag="VGB85_16750"
     CDS             complement(278..1594)
                     /locus_tag="VGB85_16750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135737.1"
                     /translation="MSSPAATELAAPAAGLPFPRLDLAWTPPMATAGDASALAAALAR
                     FPEVELQLGPRLAGAAWQQRGAALVAALSPDTRVAAVARGSLAGVLAADPALARPVRT
                     LGPNGRPRYAAPGLQPTWPEGQAMPRLVVDDATVEASAWQALPAVSVGSCEPAMQALA
                     EGQELALVQLTPFLDRVDARLAASHRARLQARLPAMQAALAAVRTSAEPACAEALRMH
                     LRAHEDCLAAATCAAAPRIVLAGGATIVAPEVEPPIPAHCAAQERDVPAQLRRISRDA
                     VAAAIPTLDPGWSTLADRLGALSEVHAALEDICTPRRRRFAAADIAEARRRLASIGVA
                     LASDERRGGRWQLADEAATVAGLGAVRVQARFAAGPASVNAGIVAEARALRDFVIARS
                     MCRSGHAAAPLAVLLRAPGAREPAFFGYFLEEELFCGALPPLGVEP"
     gene            1865..>3362
                     /locus_tag="VGB85_16755"
     CDS             1865..>3362
                     /locus_tag="VGB85_16755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135738.1"
                     /translation="MSRRPGSIPTRRLAGVGTLVLLAGMCAGDARAAEDLLVEVHFKP
                     VPNVQIAIWLEDQAGKWVQDILVTQAVGKLGIGNRPGLWNFVSSWRAPYGPRPSVLPI
                     WAHRRGKTYPKLVFFDDDPADQESLGWHENASSAETYYCRPLTPSEHEVIAVDTMTCP
                     SPAVFQTDKGVFDPKATSPYPPRNDLLDFEAGQDSPDAMKFAALNDLDAVTAATPPGN
                     MPEYVTAMIPGEAVNGPLTAWIEVSLEHDENPSHDYDREKDHYVDPRLDTYGVEYLGQ
                     PSVVYKLPFDPRDKGFTGTSSWAGYGDWNGATGTIHPPDATISTTKGSGADRLQQYTL
                     NGETFRFGVYSYGAMGGDDTSDPVDPDTGDTVDPNTGAGDTVDPDTGATASAGDNGSG
                     TGDDDGGSVGGDNGGDDGGWGHCDLHPLPAVTGLTLEGLSFDKVRVTYTLPPQTDPDF
                     MLYDVRVHLLPGETPLTEERLASAQLPMPKIVEAATPGKRGVLDVEQLW"
BASE COUNT          509 a         1305 c         1145 g          403 t
ORIGIN      
        1 gggtcctgtc ccggagaaca tgtcctcgcg cattcggcca aagctgtcac aacgctgtta
       61 gcatatccca gcagcgacat tccaggccgt ttgcgcggac gggcgagcgc tctcgagcga
      121 gaatccgcgg tccggttaac gctggtatgt gcaaatatgc gcaaagaacg ccggacgggc
      181 gctcgaaata gattgtgcac acctgaccgg atggacaggc cggcatctgt gaatatatcc
      241 gcggcgaatg agtcggccgc cgatgcagac cgaactttta cggttcgacg ccgagcggcg
      301 ggagtgcccc gcagaacagc tcctcttcga ggaagtaccc gaagaaggcg ggctcgcggg
      361 ctcccggcgc cctgagcaag acggccagcg gcgccgcggc gtggcccgag cggcacatgc
      421 tgcgggcgat cacgaagtct cgcagcgccc gcgcctcggc gacgatgccg gcgttgaccg
      481 aggccgggcc ggccgcgaag cgggcctgca cgcgcaccgc cccgagcccc gccacggtcg
      541 ccgcctcgtc ggcgagctgc cagcgacccc cgcggcgctc gtcgctcgcc agcgcgacgc
      601 cgatcgacgc cagccggcgg cgagcctcgg cgatgtcggc cgcggcgaag cggcgacgcc
      661 tcggggtgca gatatcctcg agcgccgcgt ggacctccga cagcgcgccg aggcggtcgg
      721 cgagcgtcga ccacccgggg tcgagggtcg ggatcgcagc cgcgaccgcg tcccgggaga
      781 tccgccgcag ctgggccggg acgtcgcgct cctgcgccgc gcagtgcgcg gggatcggcg
      841 gctcgacctc gggggcgacg atcgtcgcac caccggcgag cacgatccgc ggcgcggccg
      901 cgcaggtcgc cgcggccagg caatcctcgt gcgcgcgcag gtgcatccgc agcgcctcgg
      961 cgcacgccgg ctccgcgctc gtccgcacgg cggcgagcgc cgcctgcatc gcgggcagcc
     1021 gcgcctgcag ccgcgcccgg tggctcgcgg ccagccgcgc gtcgacccga tccaggaacg
     1081 gcgtcagctg gacgagcgcc agctcctgac cctccgccag cgcctgcatc gccggctcgc
     1141 agctgccgac gctgaccgcg ggcagggcct gccacgcgct cgcctcgacc gtcgcgtcgt
     1201 cgacgaccag ccgcggcatc gcctgtcctt cgggccaggt cggctgcaag cccggcgccg
     1261 catagcgcgg gcgcccgttc ggcccgagcg tgcgcaccgg ccttgcgagc gccgggtcgg
     1321 ccgcgagcac gcctgcgagg ctcccgcgag cgaccgccgc gacccgcgta tccgggctca
     1381 gcgcggcgac cagcgccgcg ccgcgctgct gccacgccgc gccggccagc cgcgggccga
     1441 gctgcagctc gacctccggg aagcgggcca gcgcggcggc cagcgccgaa gcatcgcccg
     1501 ccgtggccat cggcggggtc catgcgaggt cgaggcgggg aaacgggagc ccggccgccg
     1561 gcgccgcgag ctcggtcgcc gcgggcgagc tcacgggcgc cgccgacccc ggccgatcgc
     1621 ggccgcagcc aggtgctatc aaaaccacgc agagcgcgat cacagccgcc ccaggacccg
     1681 gtcgcccggc ccctccctgt ccccccggca tccgccatgg tcccgacggc agtgttcgca
     1741 gtgctcgcgg catggcgcgg cccgcatcgt acggcctcgg gcggaaaacc gcgaccggct
     1801 tcgctccagg ccgggcgcca ccggcgttcg ctggcccggg gggcgggacg tgtatactgc
     1861 cccgatgtcg cgccgccccg gatcgatccc cacacgacgc ctcgccggtg tcgggacgct
     1921 ggtcctcctc gcaggcatgt gcgccgggga tgcgcgcgcc gccgaggatc tgctcgtcga
     1981 ggtccacttc aagccggtgc ccaacgtaca gatcgcgatt tggctcgagg accaggccgg
     2041 caagtgggtg caggacatcc tcgtcacgca agctgtcggc aagctcggca tcggcaaccg
     2101 ccccggcctg tggaacttcg tgtcgagctg gcgggcgccc tacggcccgc ggccctcggt
     2161 gctgccgatc tgggcccatc gccgcggcaa gacctacccc aagctcgtgt tcttcgacga
     2221 cgaccccgcc gaccaggagt cgctcggctg gcacgagaac gccagctccg cggagaccta
     2281 ctactgtcgc ccgctgacgc cctccgagca cgaggtcatc gcggtcgaca cgatgacctg
     2341 tccgtcgccc gcggtgttcc agaccgacaa gggcgtcttc gaccccaagg cgacgagccc
     2401 gtatccgccg cgcaacgacc tgctcgactt cgaggccggc caggactcgc cggacgcgat
     2461 gaagttcgcc gcgctcaacg acctcgacgc cgtgaccgcc gcgaccccgc ctggcaacat
     2521 gcccgagtac gtcaccgcga tgatccccgg cgaggccgtc aacgggccgc tgaccgcctg
     2581 gatcgaggtc tcgctcgagc acgacgagaa cccctcccac gactacgacc gcgagaagga
     2641 ccactacgtc gacccgcgcc tcgacaccta cggcgtcgag tacctcggcc agccctctgt
     2701 cgtctacaag ctgcccttcg acccgcgcga caagggcttc accggcacct cgagctgggc
     2761 cggctacggc gactggaacg gcgccaccgg cacgatccac ccccccgacg ccacgatctc
     2821 caccaccaag ggcagcggcg ccgatcgtct ccagcagtac accctcaacg gcgagacctt
     2881 ccgcttcggc gtctacagct acggtgcaat gggcggcgac gataccagcg accccgtcga
     2941 ccccgacacc ggcgacacgg tggaccccaa caccggcgcg ggcgacactg tcgaccccga
     3001 caccggcgcg accgccagcg cgggcgacaa cggcagcggc accggtgacg acgacggcgg
     3061 cagcgtcggt ggtgacaacg gcggcgacga cggcggctgg ggtcactgcg acctgcaccc
     3121 gctgcccgcc gtgaccggcc tgacgctcga gggtctcagc ttcgacaagg tccgcgtcac
     3181 ctacacactc ccgccgcaga ccgaccccga cttcatgctc tacgacgtcc gcgtgcactt
     3241 gctcccgggc gagacgccgc tgaccgagga gcgcctcgcc tcggcccagc tgccgatgcc
     3301 gaagatcgtc gaggctgcga ccccaggtaa gcgcggcgtg ctcgacgtcg agcagctgtg
     3361 gg
//
LOCUS       DASWNJ010000989         2766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_510880, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000989 DASWNJ010000000
VERSION     DASWNJ010000989.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510880"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VGB85_16760"
     CDS             <1..865
                     /locus_tag="VGB85_16760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135739.1"
                     /translation="HHYAELRAREPDDVLLAREATFAANIRQHLTAAASAAPGARPLA
                     LLVVADELASSRDEAAAALRRKLLREAALQAATDAGDAVELAGELPGFYLLSAGADEA
                     ALASLTGAAAVDRRARTLYLLADATMVLRGPRVARPLYFEALLLDPFDHALRAARCEN
                     VRFLPEVAQREFTIDDEPAAWCAPVGVVTGVLPCPPVLAEHLLPGARASLPDAASPHQ
                     KACLQRARRFMQAMHIDASLDFGADDVPVIEARRTMRELAPTLFEAHMQRRDAASPRV
                     AARRPGARGGP"
     gene            909..1643
                     /locus_tag="VGB85_16765"
     CDS             909..1643
                     /locus_tag="VGB85_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135740.1"
                     /translation="MTRKRTAIVLGGSGSVGAALLRELFHDEGFDAVVTLSRRSLPEA
                     VALAGATGRSLVEKLVPVMEPAPLAAATLEAVNALEGDVEGFSVLGVGAGTAKLTLEE
                     HRAVDVALNEAFARALRDSGKVRHLAFMSAAAANPNAKASGSGAAGMSRYNRVKGESE
                     VAVRASGPAIVSVFRPAMIIGSQHTPWLLEKALPLVSFVTPAKFRSIRVEQIAKAMIA
                     TAKHHPATSATYHYPEMMALIASGQG"
     gene            1763..>2766
                     /locus_tag="VGB85_16770"
     CDS             1763..>2766
                     /locus_tag="VGB85_16770"
                     /inference="COORDINATES: protein motif:HMM:NF019151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HEY0135741.1"
                     /translation="MPRLWWMTLLVLLGCSPLGADGSHDEPLAGVEAGPAALEPAGAG
                     TALALLGALPVREHGPRDGYGREQFGPPWADADRNGCETRDDILRRDLGDVEIKPGAR
                     ACVVLSGTLEDPYTGRKIRFDRGGASEVDIDHVVALENAWVTGAAALSFRQRVALAND
                     PLNLIAAEARANRAKGASDAARWLPPNPRFHCAYAARQIAVKAKYGLWTTPAEHAALA
                     GALQRCPDEPAPASDAPTTAPIDPREPAPHTASARRPASEPGRTPATRVRPPAGEPGA
                     AGTADAKRPADPRPSEPDSPAPRRTPDPDYGSCKQVKAHGAGPYVRGRDPEYAYYDDR
                     "
BASE COUNT          369 a         1042 c         1015 g          340 t
ORIGIN      
        1 gcatcactac gccgagctgc gcgcccgcga gccggacgac gtgttgctcg cccgcgaggc
       61 cacgttcgcg gccaacatcc gccagcacct cacggcggcg gcctcggccg cacccggggc
      121 gcggccgctg gccctgctcg tggtcgccga cgagctcgcc tcgtcccgcg acgaggcggc
      181 cgcggccctg cggcgcaagc tgctgcgcga ggcggccctg caggccgcga cggacgcggg
      241 cgacgccgtc gagctcgccg gggagctgcc cgggttctac ctgctctcgg ccggcgcgga
      301 cgaggcggcg ctcgcgtcgc tgaccggggc ggccgcggtc gaccggcgcg cgcggaccct
      361 gtacctgctc gcggacgcga cgatggtgct gcgcggcccc cgcgtggccc gcccgctgta
      421 cttcgaggcc ctgctgctcg accccttcga ccacgccctg cgggccgctc gctgcgagaa
      481 cgtccgcttc ctcccggagg tcgcgcagcg ggagttcacg atcgacgacg agccggccgc
      541 ctggtgcgcg ccggtcgggg tggtgacggg ggtcttgccg tgcccgccgg tgctcgccga
      601 gcacctgctg ccgggggccc gcgcgagcct gcccgacgcc gcgagccccc accaaaaggc
      661 ctgcttgcag cgcgcccgcc ggttcatgca ggcgatgcac atcgacgcct cgctcgactt
      721 cggcgccgac gacgtcccgg tgatcgaggc ccgccggacc atgcgggagc tcgccccgac
      781 gctgttcgag gcgcacatgc agcgccgcga cgccgcgagc ccgcgggtcg ctgcgcgacg
      841 cccgggtgcg cgcggcggcc cgtaaacgcg gcccatgacg agcaactcgc gcgtatcatc
      901 gccgcgccat gacccgcaag cgcacagcga tcgtcctcgg aggctccggc tcggtcggag
      961 ccgcactgct ccgcgagttg ttccacgacg agggcttcga cgccgtcgtc acactgtcgc
     1021 ggcggtcgct gcccgaggcg gtcgcgctgg cgggcgccac cggccgcagc ctcgtcgaaa
     1081 agctcgtgcc cgtcatggag cccgcgccgc tcgccgcggc gacgctcgag gcagtgaacg
     1141 cgctcgaggg cgacgtggag ggctttagcg tgctcggcgt cggagccggc accgcgaagc
     1201 tcaccctgga agaacaccga gcggtcgacg tcgcgctcaa cgaggcgttc gcgcgggcgc
     1261 tgcgagattc gggcaaggtc cggcacctcg cgttcatgag cgccgcggct gccaacccga
     1321 acgcgaaggc cagcggcagc ggtgccgcgg gcatgtcgcg gtacaaccgc gtcaagggcg
     1381 agtcggaggt ggcggtgcgc gcgagcgggc cggcgatcgt cagcgtgttc cgcccggcga
     1441 tgatcatcgg ctcccagcac acgccgtggc tcctcgaaaa agcgctgccg ctggtttcgt
     1501 tcgtcacccc ggcgaagttc aggtcgatcc gcgtcgagca gatcgcaaag gcgatgatag
     1561 cgacagcgaa gcaccacccc gcgacgagcg cgacgtatca ctacccggag atgatggcgc
     1621 tgatcgcgag cgggcagggg tagtccgctg cgtgatatcg gggtgccgct ggcgagcgcg
     1681 cacgcaccga tcgacgtgcg ctcgaatgcg cgcagatggc ccgtggatat ctggggcgcc
     1741 cgctcgggta cactcgcggc cgatgcctcg gctgtggtgg atgaccttgc tggtcctgct
     1801 cgggtgctcg ccgctcggcg ccgacgggtc gcacgacgag ccgctggcgg gggtcgaggc
     1861 cggccctgcc gcgctcgagc cggccggcgc gggcaccgcg ctcgcgctgc tcggcgcgct
     1921 gccggtgcgc gagcacggtc cgcgcgacgg ctacgggcgc gagcaattcg ggccgccgtg
     1981 ggcggacgcg gatcgcaacg gctgcgagac ccgcgacgac atcctccgcc gcgacctcgg
     2041 cgacgtggag atcaagccgg gcgcccgcgc ctgcgtcgtc ctgtcgggca ccctcgagga
     2101 cccctatacc ggcaggaaga tccgcttcga tcgcggcggc gcctccgagg tcgacatcga
     2161 tcacgtcgtc gcgctggaga acgcgtgggt caccggcgcc gcggcgctgt cgtttcgcca
     2221 gcgcgtcgcg ctcgccaacg accccctcaa tttgatcgcc gccgaggcgc gcgcgaaccg
     2281 ggccaagggt gcctcggacg cggcgcgctg gctgccgccg aacccccgtt ttcattgcgc
     2341 ctatgcggcg cgccagatcg cggtgaaggc gaagtacggg ctgtggacga ccccggccga
     2401 gcacgccgcc ctcgcgggcg cgctgcagcg ttgccccgac gagccggcgc ccgcgagcga
     2461 cgcaccgacg accgcgccga tcgatcctcg cgagccggcg ccgcacacgg cgagcgcgcg
     2521 gcgccccgcg agcgagcccg gcaggacacc ggccaccagg gtcaggcccc ccgcgggcga
     2581 gcccggtgcg gcaggcaccg ccgacgcgaa gcgccccgca gatccacgac cgagcgagcc
     2641 cgacagcccg gcgccccggc gcacgccgga ccccgactac ggcagctgca agcaggtcaa
     2701 ggcccatggc gcgggtccgt atgtgcgcgg tcgggacccg gagtacgcct attacgacga
     2761 tcgtga
//
LOCUS       DASWNJ010000990        13986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_195550, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000990 DASWNJ010000000
VERSION     DASWNJ010000990.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 13986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13986
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195550"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1899
                     /locus_tag="VGB85_16775"
     CDS             <1..1899
                     /locus_tag="VGB85_16775"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY0135742.1"
                     /translation="ALADASALASAFTAEHRQVFGYAREGHPIEITGVRVELRELGSP
                     FQHVESDMSGAPGEPGSSELWLDGAWHTVPVWPREALRPGQRIVGPAIVAEDTATIVL
                     EPGFVAEIDRGCLLARDLAGASATPVPADARDPVLREVLSQRITAIAQQMGVVLRRTA
                     LSTNIRERLDFSCAVFDARGDLVTNAPYIPVHLGAMPESIRGVLAVHPRPPAGAVYVT
                     NDPAAGGSHLPDITVISPVHDEQGALRFFVASRGHHADVGGVAPGSMPPHATRLEEEG
                     VVFRALQVVADGRFDREAVLAVLQAGPWPARKPLENLADLEAQIAANRSGARQLHELC
                     EQYGTPLVSTAMQAIQDDAAEAVAAAIDRLPPGKHRFADAMDDGAAVVVEISRQGPRL
                     HVDFTGTAAQDPGNLNAPRAVTVSALLYVLRLLAGRTMPLNSGCLRLVDLTLPPGLLS
                     PGPGAAVAAGNVETAQRVVDVLLGALGLAAASQGTMNNLTFGDETFGYYETLGGGAGA
                     CHGHAGRSGVHTHMTNTRITDPEVLEARFPVRLWRFELRRGSGGAGRWPGGDGLVREL
                     EFLRPMHVAILSQRRLRTPFGLAGGGPGAAGRNLLDDRALPGRVAVEVAAGQRLRIET
                     PGGGGHGG"
     gene            1970..2839
                     /locus_tag="VGB85_16780"
     CDS             1970..2839
                     /locus_tag="VGB85_16780"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEY0135743.1"
                     /translation="MLEALIPYIRPPEKLIADLPVIGPLKLQFFGPLVAAGVILGYHR
                     CLHYAKRKDIDEFIARDLMFWVLVTGFVISHWVSVLFYFPEDVAANPLVLIMIWNGLS
                     SVGGFFGAMVGFFWYLRKQKQPYLVYFDMLCYGLLVGFTMGRLGCSLVHDHPGSIVDA
                     SHILAVGPWPDGQYRLDLGLLEFMYLVPIVLYIHLVYDWMKARPGKLIGTVALLYGPY
                     RFFLDTMREKDKLYMGLTTAHYASMIILALGAYLVFFRKPRPEDFAWAKDSDRIAAEQ
                     TVKAKGASTTTAP"
     gene            2943..3647
                     /locus_tag="VGB85_16785"
     CDS             2943..3647
                     /locus_tag="VGB85_16785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135744.1"
                     /translation="MSTAHRVRSGVVALAWSLLTLAGCPGNGDTDPVTTTDTTGQATT
                     TVTGTTGATDPGVTTGADTDPGASTTTTAGMTTTGASTTGAVTTTATTTTETSGAESG
                     CEPSPCEPCPKGCTPQSECVAGEWTCECIDCPPEVACNEDPPVFPDFDESCGVAEDCA
                     IVFHQIDCCGSKAAWGVNADVAKAFGDAEAMCVMQYNQCDCPTLPPVADDGKSAEDPA
                     LIQVDCNDGQCQTYVP"
     gene            3754..4032
                     /locus_tag="VGB85_16790"
     CDS             3754..4032
                     /locus_tag="VGB85_16790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135745.1"
                     /translation="MKGIFAEYGTLAIVLYLIIFAGTVAGFAVAISFGVEPDSTAGSV
                     GTLAAAWIATKLTQPLRILATLALTPLVAAGIKRLRPGKPDASPPGPG"
     gene            complement(4039..5133)
                     /locus_tag="VGB85_16795"
     CDS             complement(4039..5133)
                     /locus_tag="VGB85_16795"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY0135746.1"
                     /translation="MQATTPFLFCVCQTGAEGAVTEELVRARPDLRKSFSRPGLITFK
                     GGVQFSAEVVVDAVFVRAFGASIGPAKDLDAVVAAVQRYCPEGQAVRLHVWERDVAKP
                     GDEPDGHVYGPRADEARAQIEAAWERTGRPLLAGSRAETGDWVLDVIVAGDEPWWLGF
                     HRHGPGHAPTPGGRLTVDMPPEAPSRAYRKLEEALMWSEAPLRAGDTAVEIGSSPGGA
                     SYALLRRGVEVYGIDPAKMAPVVLDFNKDQPGNHFVHLDRPMSMVQRADLPTELHWVL
                     LDVNLAPQVALITARRLAAHPRPALMGVLLTLKLDDWKAVHHVPRFLKSISSMGMLEV
                     KATQLPSNRMEIFVCGLTKRGLERRDGVTK"
     gene            5213..6358
                     /locus_tag="VGB85_16800"
     CDS             5213..6358
                     /locus_tag="VGB85_16800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEY0135747.1"
                     /translation="MSRDHWTPEFLDAMRLVGDPPADAVVAELFAGGANVVHAVNYLM
                     RDLVENDDVPSGALPGHVRDYFAATPLPAWADPLRLHRGSELFHRYGPMVVMLLNLYS
                     LPLTYAAKKGVQVLARTDRLHSNSKRRIVETAQMIIDVMAPGGLDPASRGAGLRSAQK
                     VRLMHATVRHLLGQDPTWDADLGVPINQEDLAGTLGAFSVAIIDGLQRLGVDLGAAEI
                     DAYMHTWCVVGHVMGVREELLPTDHAAGLRLVAQIGERHFAACPEGQMMTAALLDMMT
                     TLVPGAAFDAVPGQLVRFFVGERVAQILALPDLPGPHGESPLVQALRALGHAGDVAGD
                     RSGLVQALARRFSGSLLDGMMLVSRGGQRLPFNIPTELRQVWGANWR"
     gene            6625..8607
                     /locus_tag="VGB85_16805"
     CDS             6625..8607
                     /locus_tag="VGB85_16805"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEY0135748.1"
                     /translation="MLALRNVLLTLTCAVMLSACARQMEDVRGVESYIRKIELEGVNR
                     FKKKELLSYLNIGESSRLIWRPRYVFTEAMLPIDAERIVELYRAYGYYDAEVVSMVPD
                     TKVGRLRLFGKHKGERKPGKTRIKIVVREGAPTKVNDLELHWPEGPPAGPPDEHATED
                     AIAESALLKEGEPFEVPKLNTSTQLLKGHMQDRGYAFAQVKDKAVIDPGRGVEVDFEL
                     RPGPFVKIGTIKIEGLVGVPEKYVRNEIDFAPGKRYSPSLLTRIEQKIYGMDVFQSVS
                     IDAADKPNDKGEIDLTIRVVESKTQSIKIGPGIGIDPVRWEQRATALYSHKNFGHNLT
                     RFDMRVTAGYAELPSLFNPQAHGPLLRIDPKIQQKGFLEKRTTWTLAPSFELGIWQGY
                     QFYSPQLRMGPSRFFGKYTEVSLTYNLRYVDFFNVSEALRLDDNILGLDFRDPYFVSY
                     LEPQVNFYFVDSILKPRNGAIIGATYDIAGLGGDFTFHKIQPTIRGYYAPHRRLLLAA
                     RASVGFMFPFEPKAETPIDMRFYLGGSDTVRGWGLRRLSPKVFLADCESDNTGACEDG
                     VPTGGQTMILANLEARVLIVDKLSMVGFFDMGDVRGAVRTFSPKNWNYSVGPGVRFDS
                     PIGVFRLDVGFRLNETDQSAAEDRIWAVHFGLGEAF"
     gene            8610..13667
                     /locus_tag="VGB85_16810"
     CDS             8610..13667
                     /locus_tag="VGB85_16810"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEY0135749.1"
                     /translation="MAAGRARRIVRKTLRWSLRVFLSLLLLVLVVVVLLLYSPGFLHF
                     ALNIGLGFYNGRIPGEIRIGRVEGRLADHLVLGDIYLEDAEGNVLVAARELSLTWHPW
                     DLLHKGVTVDKLALSDAQIHLVKGAGFGDLAIPGPAKPKRQTVAPDLPIDLRVGEIEI
                     QGVDLFKPTGETIVGELRLSARDLTWKGLVAHLKIDDAAGKLPGVALEALALEADYQE
                     PHLGLTGLITTDMAIAELVRVDLDADDLTGEVLLAVDGRRGAVAAKLKGPLAEKIANA
                     PGDPSVRIDARGKLGHFEIGVQALVPGVGGLELSASGNPIGAPHLDLRGRVDADLAGL
                     LPPSLADKLGYVRPTFEARLTGPDWQQLSTELRLSCVDCGAASGLDVRALARRDAILG
                     DASAFAQLVGAGMALTGEVQLGPQGPEAGRWDLRIPDVAVPLGVARLFAELPPIDGSL
                     TTRGSCTGAPLRCEGFVEVDHFRGYNARVGAVRAQLHGEPLAPVPVAHAQLSIRDLGV
                     PNYHFAGAEITADVGPSKDVPIDVEHPHGALEAAIDLEAWVRQRDRGDRAKVVARVRP
                     GPPLTILLDALDVHMRGLQALLPRPARVEIAGRRIDIKGLRLLAAGGEIGVDGRADLD
                     GRSDLKATLDRIKLAPLVGLVPNLRGQVSGTVTARVSLQGPAQSPEIGLQLGAAKLRF
                     REGVIGDIDLGVGVGRGKGRVALGVTGPMAERFAIGAEVPLTADLSRSKFATRQGPLH
                     FAVDIKNFRLASLRPWIKGSFAPHGTVDVQVAVDGPASKPDTKIAVRGRHLVFQTEAP
                     ASLDIDFEQKAGEDAKARVDIIRLGGRLRINAPVLPMRVNLVDDGIKWRPEAQHEVTI
                     VAREIDLWRQLNAFMPGHDFAGRLEVEGRLSGPMTDPKLQIAVMGENLRFRDGLIGKL
                     RLDTYLADQRATVDLKVNGDVAKQITLHAEAPLQVELAKGKVAWLKDQPHLVDAQIRG
                     VDLAGLKRLGLTAAWEGGVDVTAKLRGSASVPEFNVHTDLKEIIWKSRRVGTVRADID
                     YAKKQVKLGVDAQLGRGTIKARGTAPVAMDLGRNDFKWDENGQHDIEIRVDQLDRTML
                     APLGRVPEEALLELSMIAKAKGNLADFAASLEAHGQMGHKLIGGAPVHISADILPKSQ
                     ALKFSVGPHSWAGEIKVAVNSKADILALVRKTAKADDIAFTASMRAPKFDTRFAQAFV
                     PRELYDVNGELKATIDATGTLGAPQIKGDLHMRRGSISVLQMQQRIRKIDFDVKADGR
                     TIVLEKFTAESGRGRVTANARVELPVGGGMKLNAETKLSKFPLVRPGLPQMQIDTHVK
                     AKMVSTAEETDINLGIGGTKVYVTGYTVDPPKQIPESPSVSFKDDKLRVAVATEEGPD
                     GEVSAENEVKNPKPDKNKAGKRFAINIKLTDPVDIRGPGTEMLWQGAVAATREGEKRE
                     VTGKLTAKEGRLDLLGNRFKIQSGEVTLPEDEDTVDPYIHVVATTSTVVAEVTATFKG
                     RITRPSLIFSSEPAMSQSQILTLLLTGSPDASEADESRVLAQAAALLATFQNPALSSF
                     LSSRLGIDHVGLSFGDDVNQPILSVGKNLTKNIYVETAYKVNADPRRSNKVEARVEYQ
                     FKPRWSVETFFGDAAVGGLDVFWRKIFGQPKRLKEHEGKPTPKGTAAGDSPRGGKAAD
                     APSAGGSQMGGGGGGAK"
     gene            complement(13671..>13986)
                     /locus_tag="VGB85_16815"
     CDS             complement(13671..>13986)
                     /locus_tag="VGB85_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135750.1"
                     /translation="LLVDLDASGTGTVGTVEIAMVPEGLTIAGPASWSGEVVAGTGKQ
                     LRFPLQLITDKVARVTITHGIAGAPANDPVVFRFVVEDEEIRACQASEEACKEPGANP
                     AP"
BASE COUNT         2101 a         5158 c         4717 g         2010 t
ORIGIN      
        1 gcgctcgcgg acgcgtccgc cctcgctagc gccttcaccg ccgagcaccg ccaggtcttc
       61 ggctacgcgc gcgagggcca cccgatcgag atcaccggcg tgcgcgtcga gctgcgcgag
      121 ctcgggagcc cgtttcaaca tgtcgaatcg gacatgtccg gagcgccagg tgagccgggc
      181 agctcggaat tatggctcga cggcgcctgg cacactgtcc cggtgtggcc gcgcgaggcg
      241 ctgcggcccg gccagcgcat cgtcggtccg gcgatcgtcg ccgaggacac cgcgacgatc
      301 gtgctcgagc ccggctttgt cgccgagatc gatcgtggtt gcctcctcgc ccgcgacctc
      361 gcgggagcca gcgcgacgcc cgtgcccgcc gacgcccgcg acccggtgct gcgcgaggtg
      421 ctgtcgcagc gcatcaccgc gatcgcgcag cagatggggg tggtcctgcg acgcacggca
      481 ctgtcgacca acatccgcga gcgcctcgac ttctcgtgcg ccgtgttcga cgcacgcggc
      541 gacctcgtca ccaacgcgcc gtacatccca gtgcacctcg gcgcgatgcc ggagtcgatc
      601 cgcggcgtgc tcgcggttca tccgcgtccg ccggccgggg ccgtctacgt caccaacgac
      661 ccggccgccg gcggctcaca cctgcccgac atcaccgtga tctcgccggt gcacgacgag
      721 cagggcgcgc tgcgcttctt cgtcgccagc cgcggacacc acgcggacgt cggtggggtg
      781 gccccgggct cgatgccccc gcacgcgacg cggctggagg aggagggcgt ggtgttccgc
      841 gcgctgcagg tcgtggcgga cggccgcttc gaccgcgagg ccgtgctggc ggtgttgcag
      901 gccggcccgt ggccagcgcg caagcccctc gagaacctcg ccgacctcga ggcgcagatc
      961 gccgccaatc gctccggcgc ccgccagctc cacgagctgt gcgagcaata cggcacgccg
     1021 ctggtctcta ctgcgatgca ggcgatccag gacgacgccg ccgaggcggt cgccgccgcg
     1081 atcgaccgcc tgccgccggg caagcacagg ttcgccgacg cgatggacga cggcgccgcg
     1141 gtcgtcgtcg agatctcgcg gcagggcccg cggctgcacg tcgacttcac cggcacggcc
     1201 gcccaggacc cgggtaacct caacgcgccg cgggcggtca ccgtgtcggc gttgttgtat
     1261 gtgctgcgac tgctcgccgg gcgcacgatg ccgctcaact ccggctgcct gcgcctggtc
     1321 gacctgactc ttccgccagg tctgctgtcc ccggggccag gtgccgcggt cgcggccggc
     1381 aacgtcgaga cggcgcagcg ggtggtcgat gtgctgctcg gcgcgctcgg gctcgcggcg
     1441 gcgagccagg gcacgatgaa caacctgacc ttcggcgacg agacctttgg ttactacgag
     1501 acgctcggcg gcggcgcggg tgcgtgtcat gggcacgcgg gtcgcagcgg cgtgcacacg
     1561 cacatgacta acacgaggat caccgacccg gaggtgctcg aggcgcgctt cccggtcagg
     1621 ctgtggcgct tcgagctgcg ccgcggctcg ggcggcgcgg gccggtggcc gggcggcgac
     1681 ggcctcgtgc gcgagctcga gttcctgcgc cccatgcatg tggcgatcct ctcgcagcgc
     1741 cggctccgga cgccgttcgg gctcgccggc gggggcccgg gggccgcggg acgaaacctg
     1801 ctggacgatc gtgcgctgcc cggtcgggtg gccgtggagg tcgccgcggg ccagcgcctg
     1861 cgcatcgaga ccccgggcgg gggcggccac gggggttgac cctggcccgc cccgccgcgc
     1921 acggggggtt ttgcgtgctg ctccccgctg agttacggtg cggccctcca tgctcgaagc
     1981 actgatcccc tacatccggc cgcccgagaa gctgatcgcc gacctgccgg tcatcggacc
     2041 gctcaagctt cagttcttcg gcccgctcgt cgcggccggc gtgatcctcg ggtaccaccg
     2101 ctgcctgcac tacgccaagc gcaaggacat cgacgagttc atcgcccgtg acctgatgtt
     2161 ctgggtgctc gtcaccggct tcgtcatctc gcactgggtg tcggtgctgt tctatttccc
     2221 ggaggacgtc gccgccaacc cgctcgtgct gatcatgatc tggaacggcc tgtcgagcgt
     2281 cggcgggttc ttcggcgcga tggtcgggtt cttctggtac ctgcgcaagc agaagcagcc
     2341 gtacctcgtg tacttcgaca tgctctgcta cggcctgctg gtcggcttca cgatgggccg
     2401 cctcggctgc tcgctcgtcc acgaccaccc gggcagcatc gtcgacgcct cgcacatcct
     2461 cgcggtcggc ccctggcccg acggccagta tcgcctcgac ctcggtctgc tcgagttcat
     2521 gtacctcgtg ccgatcgtgc tctacatcca cctcgtctac gactggatga aggcccgccc
     2581 cggcaagctg atcggcaccg tcgccctgct gtacggaccc taccgcttct tcctcgacac
     2641 gatgcgcgag aaggacaagc tgtacatggg cctgaccacc gcgcactacg cctcgatgat
     2701 catcctggcg ctgggcgctt acctggtgtt ctttcgcaag cccaggcccg aggacttcgc
     2761 ctgggccaag gacagcgacc gcatcgccgc cgagcagacc gtcaaggcca agggcgcctc
     2821 gacgacgacc gctccgtgag ccgccctgtg tccctcgggc gatgggcccg agggacaggc
     2881 ctcgggcccc acgcccgctc gccaaacttg cgggcgacgc cacccgcggc gatactgacg
     2941 cgatgtccac cgcacatcgt gtccgcagcg gcgtcgtcgc gctcgcctgg tcgctgctga
     3001 cgctcgccgg ctgccccggc aacggcgaca ccgaccccgt gacgaccacg gacacgacag
     3061 gccaggcgac cacgacagtc acggggacca ccggcgcgac cgacccgggg gtgaccactg
     3121 gcgccgacac ggaccccggc gcatcgacga cgacgaccgc cggcatgacg accaccgggg
     3181 cctcgacgac gggcgcggtg acgacgaccg cgacgacgac caccgagacc tcgggcgccg
     3241 agagcggttg cgagccgagc ccgtgcgagc cgtgtccgaa gggttgcacg ccccagagcg
     3301 agtgcgtcgc cggcgagtgg acctgcgagt gcatcgactg tccccccgag gtcgcatgca
     3361 acgaggaccc gccggtgttt ccggacttcg acgagagctg cggggtcgcc gaggactgcg
     3421 cgatcgtctt tcaccagatc gactgctgcg gcagcaaggc cgcgtggggc gtcaacgccg
     3481 acgtcgcgaa ggcgttcggc gacgccgagg cgatgtgcgt catgcaatac aaccagtgcg
     3541 actgcccgac cttgccgccg gtcgccgacg acggcaagag cgccgaggac ccggccctca
     3601 tccaggtcga ctgcaacgac ggccagtgcc agacctacgt gccctgagcg cgatcgcagc
     3661 gcgtcacggc ttgttgcgag cgcgccggtc tgtcagcctg tcccgagcat ggcgagcccg
     3721 gaagatcccc cacgtccctc cctgtccgac cggatgaagg ggatcttcgc cgagtacggg
     3781 accctggcga tcgtcctcta cctgatcatc ttcgcgggca cggtcgcggg cttcgccgtg
     3841 gcgatctcgt tcggcgtcga gccggactcg accgccggca gcgtcggcac gctggccgcc
     3901 gcctggatcg ccaccaagct gacccagccg ctgcgcatcc tcgcgaccct cgcgctgacg
     3961 cccctggtcg ccgccgggat caagcggctg cgccccggca agcccgacgc ctcgccgccg
     4021 ggccccggct gagcgcgctc acttcgtgac gccgtcgcgc cgctccagcc cgcgcttggt
     4081 caggccgcac acgaagatct ccatgcggtt gctcggcagc tgggtcgcct tcacctcgag
     4141 catgcccatc gagctgatgc tcttgaggaa gcgcggcacg tggtgcacgg ccttccagtc
     4201 gtcgagcttg agcgtcagca gcacgcccat cagcgccggg cgcgggtggg cggcgagccg
     4261 gcgcgcggtg atcagggcga cctgcggcgc gaggttgacg tcgagcagca cccagtgcag
     4321 ctcggtcggc aggtccgcgc gctggaccat gctcatcggc cggtcgaggt ggacaaaatg
     4381 gttgccgggc tggtctttat taaaatcgag gacgaccggg gccatcttcg ccgggtcgat
     4441 gccgtacacc tcgacgccgc ggcgcagcag cgcatagctc gccccgcctg gcgagctgcc
     4501 gatctcgacc gcggtgtcgc cggcgcgcag cggcgcctcg gaccacatca gcgcctcctc
     4561 gagcttgcgg tacgcgcgtg acggggcctc cggcggcatg tcgaccgtca gccgaccgcc
     4621 aggcgtcggc gcgtggccgg ggccgtggcg gtgaaagccg agccaccacg gctcgtcgcc
     4681 ggcgacgatg acgtcgagca cccagtcgcc cgtctcggcg cgcgagcccg cgagcagcgg
     4741 ccgcccggtc cgctcccacg ccgcttcgat ctgcgcgcgg gcctcgtccg cgcgcgggcc
     4801 gtacacgtgc ccatcgggct cgtcgccggg cttggcgacg tcgcgctccc acacgtgcag
     4861 gcgcaccgcc tgtccttcgg gacagtagcg ctgcactgca gcgaccacgg cgtcgaggtc
     4921 cttggccggc ccgatcgacg cgccgaaggc ccgcacgaac accgcgtcga cgacgacctc
     4981 cgccgagaac tgcacgccgc ccttgaaggt gatcaggccc gggcgggaga acgacttgcg
     5041 caggtccggg cgggcgcgca cgagctcctc ggtgacggcc ccctcggcgc cggtctggca
     5101 cacgcaaaac aggaaggggg tggtcgcttg cacgcgcggc agtcataggc cagcggcgcc
     5161 cccggttcaa gggcgcagtc gcccgtcgcg gcgtgttagc gtcgctgccg ggatgtcgcg
     5221 cgaccactgg acccccgaat tcctcgacgc catgcgcctg gtcggcgacc cgcccgcaga
     5281 cgcggtcgtc gccgagctgt tcgccggcgg cgccaacgtc gtgcacgcgg tcaactacct
     5341 gatgcgcgac ctcgtcgaga acgacgacgt gccgtcgggc gcgctgcctg ggcacgtgcg
     5401 cgactacttc gcggccacgc ccttgccggc ctgggccgac ccgctgcggc tgcaccgcgg
     5461 cagcgagctg ttccaccgct acggcccgat ggtcgtcatg ctgctcaacc tctactcgtt
     5521 gccgctgacc tacgcggcga agaagggcgt gcaggtgctc gcccgcaccg accgcctgca
     5581 ctcgaattcg aagcgccgga tcgtcgagac ggcgcagatg atcatcgatg tcatggcgcc
     5641 cggcggcctc gacccggcct cgcgcggcgc cggcctgcgc tcggcccaga aggtccgact
     5701 gatgcacgcg accgtccgcc acctgctcgg ccaggacccg acctgggacg cggacctcgg
     5761 ggtgccgatc aatcaagagg acctcgccgg cacgctcggc gccttctcgg tcgcgatcat
     5821 cgacggcctc cagcgcctcg gcgtcgacct cggcgccgcc gagatcgacg cgtacatgca
     5881 cacctggtgc gtcgtcggcc acgtcatggg cgtgcgcgag gagctgctgc cgaccgacca
     5941 cgccgccggc ctccggctgg tcgcgcagat cggcgagcgc cactttgcgg cctgtcccga
     6001 gggccagatg atgaccgccg cgctgctcga catgatgacc acgctcgtcc cgggcgccgc
     6061 cttcgacgcg gtgcccggcc agctggtccg gttcttcgtc ggcgagcgag ttgcgcagat
     6121 cctggcgctg ccggatctcc cgggacctca tggcgagagc ccgctcgtgc aagccttgcg
     6181 cgcgctcggc cacgccggtg acgtcgccgg cgaccgctcg ggcctcgtgc aggcgctcgc
     6241 gcggcgcttt agcggctcgc tgctcgacgg catgatgctg gtcagccgcg gcgggcagcg
     6301 cctgcccttc aacatcccga ccgagctgcg ccaggtgtgg ggcgccaact ggcgctgagc
     6361 cggggccggc cgaagcccga tcccgggatc gtggccggtg gaaattccca tgaactatca
     6421 aagctcgcac gggcgatcgc ggagtccgcg gacatcgttt cgccgtcgcg gggccggggg
     6481 ggcggggccc cggggttacc cgcctcacag cgcccacggc accggtgaac ggaccggcca
     6541 ggagcctgga aaccgtccca ggcggccacc ctcgccatgg cgccgtgagt gcgctttgcg
     6601 cgacccgggc tattaaccgg cccggtgctc gccctgcgaa acgtcctgct gacgctgacc
     6661 tgcgcggtca tgctctccgc ctgcgcgcga cagatggagg acgtgcgcgg cgtggagtcc
     6721 tacatccgga agatcgagct cgagggcgtc aaccgcttca agaagaagga gctgctgtca
     6781 tatctcaaca tcggtgagag ctcgcgcctg atctggaggc cgcgctacgt gttcaccgag
     6841 gcgatgctgc cgatcgacgc cgagcgcatc gtcgagttgt accgcgccta cggctattac
     6901 gacgccgagg tggtgtcgat ggtgccggac accaaggtcg gccgcctccg gctgttcggc
     6961 aagcacaagg gcgagcgcaa gcccggcaag acgcggatca agatcgtcgt ccgcgagggc
     7021 gcgccgacca aggtcaacga cctcgagctg cactggcccg agggcccccc cgccggtccg
     7081 ccggacgagc acgcgaccga ggacgcgatc gccgagtcgg ccctgctcaa ggagggtgag
     7141 cccttcgagg tccccaagct caacacctcg acgcagctgc tcaagggcca catgcaggac
     7201 cgcggctacg cgttcgcgca ggtcaaggac aaggcggtca tcgaccccgg ccgcggcgtc
     7261 gaggtcgact tcgagctgcg ccccggcccg ttcgtgaaga tcggtacgat caagatcgag
     7321 ggtctcgtcg gcgtgccgga gaagtatgtc cgcaacgaga tcgacttcgc cccgggcaag
     7381 cgctactcgc cgtcgctgct gacccgcatc gagcagaaga tctacggcat ggacgtgttc
     7441 cagtcggtgt cgatcgacgc cgcggacaag cccaacgaca agggggagat cgacctgacg
     7501 atccgcgtcg tcgagtcgaa gacccagtcg atcaagatcg ggcccggcat cggcatcgac
     7561 cccgtgcgct gggagcagcg cgccacggcc ctctactcgc acaagaactt cggccacaac
     7621 ctgacgcgct tcgacatgcg cgtcaccgcc ggttacgccg agctgccgtc gctgttcaac
     7681 ccgcaggccc acggcccgct gctgcgcatc gaccccaaga tccagcaaaa gggcttcctc
     7741 gagaagcgca cgacctggac gctcgcgccc tcgttcgagc tcggcatctg gcagggttac
     7801 cagttctact cgccgcagct gcgcatgggc ccgtcgcgct tcttcggcaa gtacaccgag
     7861 gtctcgctga cctacaacct gcgctacgtc gacttcttca acgtgtcgga ggcgctgcgc
     7921 ctcgacgaca acatcctcgg gctcgacttt cgcgacccgt acttcgtcag ttatctcgag
     7981 ccgcaggtca acttctactt cgtcgactcg atcctcaagc cgcgaaacgg cgcgatcatc
     8041 ggggcgacct acgacatcgc cggcctcggc ggcgacttca ccttccacaa gatccagccg
     8101 acgatccgcg gctactacgc gccgcaccgg cggctcctgc tcgccgcgcg cgcctcggtc
     8161 ggcttcatgt tccccttcga gcccaaggcc gagacgccga tcgacatgcg cttttacctc
     8221 ggcggctccg acaccgtgcg cggctggggc ctgcgccgcc tgtcgcccaa ggtgttcctc
     8281 gccgactgcg agtccgacaa caccggcgcc tgcgaggacg gcgtgccgac cggcggtcag
     8341 acgatgatcc tcgccaacct cgaggcccgc gtgctcatcg tcgacaagct gagcatggtc
     8401 ggcttcttcg acatgggcga cgtgcgcggc gcggtgcgca ccttcagccc gaagaactgg
     8461 aattactcgg tcggtccagg cgtccgcttc gacagcccga tcggcgtgtt tcgcctggat
     8521 gtcggctttc gcctcaacga gaccgatcag tcggccgccg aggaccgtat ctgggccgtg
     8581 cactttggcc tgggggaggc gttttaatca tggctgctgg acgcgcccga aggatcgttc
     8641 gaaagacgct gcgctggtcg ctgcgggtct ttctgtcgct gctgctgctg gtgctcgtcg
     8701 tggtggtcct gctgctgtac tcgccgggct tcctgcactt cgcgctcaac atcggcctcg
     8761 gcttctacaa cggccgcatc ccgggcgaga tccgcatcgg gcgcgtcgag ggccggctcg
     8821 ccgaccacct cgtgctcggc gacatctacc tcgaggacgc cgagggcaac gtcctcgtcg
     8881 ccgcgcgcga gctctcgctg acctggcacc cgtgggacct gctgcacaag ggcgtgacgg
     8941 tcgacaagct cgccttgtcg gacgcgcaga tccacctcgt caagggcgca ggcttcggtg
     9001 accttgcgat cccggggccg gccaagccca agcgccagac cgtcgcgccc gacctcccga
     9061 tcgaccttcg cgtcggcgag atcgagatcc agggcgtcga cctcttcaag ccgaccggcg
     9121 agacgatcgt cggcgagctg cggctgtccg cgcgcgacct gacctggaag ggcctcgtcg
     9181 cccacctgaa gatcgacgac gccgccggca agctgcccgg cgtcgcgctc gaggcgctcg
     9241 cgctcgaggc cgactatcaa gagcctcacc tcggcctcac cggcctgatc accaccgaca
     9301 tggcgatcgc cgagctggtc cgcgtcgacc tcgacgccga cgacctcacc ggcgaggtcc
     9361 tgctcgccgt cgacggccgc cgcggcgcgg tcgctgccaa gctcaagggc cccctcgcgg
     9421 agaagatcgc caacgcgccc ggcgacccct cggtgcgcat cgacgcccgg ggcaagctcg
     9481 gacacttcga gatcggcgtg caggcgctgg tccccggcgt cggcggcctc gagctctcgg
     9541 cctccggcaa cccgatcggc gcgccgcacc tcgacctgcg cggccgcgtc gacgccgacc
     9601 tcgcgggcct gctgccgccc tcgctcgccg acaagctcgg ctacgtgcgc ccgaccttcg
     9661 aggccaggct gaccggcccc gactggcagc agctgagcac cgagctgcgg ctcagctgcg
     9721 tcgactgcgg cgcggcgtcg ggcctcgacg tgcgcgcgct ggcccgccgc gacgcgatcc
     9781 tcggcgacgc ctccgcgttc gcgcagctgg tcggcgccgg catggcgctg acgggcgagg
     9841 tccagctcgg cccgcaaggg cccgaggccg gccgctggga cctgcggatc cccgacgtcg
     9901 ccgtgccgct cggcgtggcg cgcctgttcg ccgagctccc gccgatcgac ggctcgctga
     9961 ccacgcgcgg ctcgtgcacc ggcgccccgc tgcgctgcga gggcttcgtc gaggtcgacc
    10021 acttccgcgg ctacaacgcc cgcgtcggcg cggtgcgagc gcagctgcac ggcgagccgc
    10081 tcgcgccggt gccggtcgcc cacgcgcagc tgtcgatccg cgacctcggc gtgccgaact
    10141 atcacttcgc cggcgccgag atcacggccg acgtcggccc gagcaaggat gtcccgatcg
    10201 acgtcgagca cccgcacggc gcgctcgagg ccgcgatcga cctcgaggcg tgggtccgcc
    10261 agcgcgaccg cggggaccgg gccaaggtcg tcgcgcgcgt gcggcccggc ccgccgctga
    10321 cgatcctgct cgacgcgctc gacgtgcaca tgcgcggcct ccaggccctg ctgccgcgcc
    10381 cggcccgcgt cgagatcgcc ggtcgcagga tcgacatcaa gggcctgcgc ctgctcgccg
    10441 ccggcggtga gatcggcgtc gatggtcgcg ccgacctcga cggtcgcagc gacctcaagg
    10501 cgaccctcga ccgcatcaag ctcgcgccgc tcgtcggcct cgtacccaac ctgcgcggcc
    10561 aggtcagcgg caccgtcacc gcccgcgtct cgctgcaggg gcccgcgcag tcgcccgaga
    10621 tcggcctgca gctcggcgcc gccaagctgc gctttcgcga gggcgtcatc ggcgacatcg
    10681 acctcggcgt cggcgtcggc cgcggcaagg gccgcgtggc gctcggcgtg accgggccga
    10741 tggcggagcg ctttgcgatc ggcgccgagg tgccgctgac ggccgacctc tcgcggagca
    10801 agttcgcgac ccgtcagggc ccgctgcact tcgcggtcga catcaagaac ttccgcctcg
    10861 cctcgctgcg cccgtggatc aaggggtcgt tcgcgccgca cggcaccgtc gacgtccagg
    10921 tcgcggtcga cggcccggcc tcgaagccgg acaccaagat cgccgtgcgc ggccgtcacc
    10981 tcgtgttcca gaccgaggcg ccggcgtccc tcgacatcga cttcgagcag aaggccggcg
    11041 aggacgccaa ggcccgcgtc gacatcatcc gcctcggcgg tcgcctgcgc atcaacgccc
    11101 cggtgctgcc gatgcgcgtc aacctcgtcg acgacggcat caagtggcgc cccgaggccc
    11161 agcacgaggt cacgatcgtc gcccgcgaga tcgacctgtg gcgccagctc aacgccttca
    11221 tgcccggcca tgacttcgcc ggccgcctcg aggtcgaggg ccggctctcg ggcccgatga
    11281 ccgacccgaa gctgcaaatc gccgtcatgg gcgaaaacct gcgctttcgt gacggtctga
    11341 tcggcaagct ccgcctcgac acttacctcg cggaccagcg cgccaccgtc gacctcaagg
    11401 tcaacggcga cgtcgccaag cagatcaccc tgcacgccga ggcgccgttg caggtcgagc
    11461 tggccaaggg caaggtcgcc tggctcaagg accagccgca cctggtcgac gcccagatcc
    11521 gcggggtcga cctcgccggt ctcaagcggc tcgggctcac ggccgcgtgg gagggcggcg
    11581 tcgacgtcac cgccaagctg cgcggctcgg cgagcgtgcc ggagttcaac gtccacaccg
    11641 acctcaagga gatcatctgg aagagccggc gcgtcggcac ggtccgcgcg gacatcgact
    11701 acgccaagaa gcaggtcaag ctcggcgtcg acgcgcagct cggacgcggc acgatcaagg
    11761 cccgcggcac cgcgccggtg gcgatggacc tcggccgcaa cgacttcaag tgggacgaga
    11821 acggccagca cgacatcgag atccgggtcg accagctcga ccgcacgatg ctcgcgccgc
    11881 tcggccgggt gccggaggag gcgctgctcg agctgagcat gatcgccaag gccaagggca
    11941 acctcgccga cttcgccgcc agcctcgagg cgcacggaca gatgggccac aagctgatcg
    12001 gcggcgcccc ggtgcacatc agcgccgaca tcttgcccaa gagccaggcg ctcaagttct
    12061 cggtcggccc gcacagctgg gccggcgaga tcaaggtcgc cgtcaacagc aaggccgaca
    12121 tcttggcgct ggtccgcaag accgccaagg ccgacgacat cgcgttcacc gcgagcatgc
    12181 gcgcgccgaa gttcgacacg cgcttcgccc aggcctttgt gccgcgcgag ctctacgacg
    12241 tcaacggcga gctcaaggcc acgatcgacg cgaccggcac gctcggcgcg ccgcagatca
    12301 agggcgacct gcacatgcgc cgcggcagca tctccgtgtt gcagatgcag cagcggatcc
    12361 gcaagatcga cttcgacgtc aaggccgacg gacggacgat cgtgctcgag aagttcaccg
    12421 ccgagagcgg caggggccgc gtcaccgcca acgccagggt cgagctgccg gtcggcggcg
    12481 gcatgaagct caacgcggag accaagctga gcaagttccc gctggtccgc ccgggcctgc
    12541 cgcagatgca gatcgacacc cacgtcaagg ccaagatggt ctcgaccgcc gaggagaccg
    12601 acatcaacct cgggatcggc ggcaccaagg tctacgtcac cggctacacg gtcgacccgc
    12661 cgaagcagat ccccgagagc ccctcggtca gcttcaagga cgacaagctg cgcgtcgccg
    12721 tcgccaccga ggagggcccc gacggcgagg tctcggccga gaacgaggtc aagaacccga
    12781 agccggacaa gaacaaggcc ggcaagcgct ttgcgatcaa catcaagctg accgacccgg
    12841 tcgacatccg cggccccggc accgagatgt tgtggcaggg cgcggtcgcg gcgacccgcg
    12901 agggtgagaa gcgcgaggtc accggcaagc tgaccgccaa ggagggtcgc ctcgacctgc
    12961 tcggcaaccg cttcaagatc cagtcgggtg aggtgacgct gcccgaggac gaggacaccg
    13021 tcgacccgta catccacgtg gtcgcgacga cgagcaccgt ggtcgccgag gtgaccgcga
    13081 ccttcaaggg tcgcatcacc cggccgtcgc tgatcttcag ctcggagccg gcgatgtcgc
    13141 agagccagat cctcacgctg ctgctgaccg gctcgcccga cgcgagcgag gccgacgagt
    13201 cgcgcgtgct ggcgcaggcg gccgcgctgc tggcgacctt ccagaacccg gcgctgtcga
    13261 gcttcctcag ctcgcgcctc ggcatcgacc acgtcgggct cagcttcggc gacgacgtca
    13321 accagccgat cctctcggtc ggcaagaact tgaccaagaa catctacgtc gagaccgcct
    13381 acaaggtgaa cgccgacccg cgccggtcca acaaggtcga ggcccgcgtc gagtatcagt
    13441 tcaagccgcg ctggtcggtc gagaccttct tcggcgacgc ggcggtcggc ggcctcgacg
    13501 tgttctggcg caagatcttc ggccagccca agcgcctcaa ggagcatgag ggcaagccga
    13561 cgcccaaggg caccgccgcg ggcgactcgc cgcgcggcgg caaggcggcc gacgctccct
    13621 cggccggcgg cagccagatg ggcggcggcg gtggcggggc caagtgaggt tcaaggagcc
    13681 gggttcgcgc ccggctcctt gcacgcctct tcactggcct gacacgcgcg gatctcctcg
    13741 tcctcgacca cgaagcggaa caccaccggg tcgttcgcgg gcgcgcccgc gatgccgtgg
    13801 gtgatcgtca cgcgcgcgac cttgtcggtg atcagctgca gcgggaagcg cagctgcttg
    13861 ccagtgcccg ccacgacctc gccgctccag ctcgcgggcc cagcgatcgt caaaccctcc
    13921 ggcaccatgg cgatctcgac ggtgccgacg gtgccggtgc cgctggcgtc gaggtcgacc
    13981 agcaac
//
LOCUS       DASWNJ010000991         3517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_400640, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000991 DASWNJ010000000
VERSION     DASWNJ010000991.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400640"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /locus_tag="VGB85_16820"
     CDS             <1..1043
                     /locus_tag="VGB85_16820"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HEY0135751.1"
                     /translation="RHDRRVQSPGHIASIAVRSVLDGLRAIGAEPAQVLRPLGVASAL
                     AEGDERWLPHAMSDALWRVACERMGPMLPLQLAEHLPHERCGHLGAMAVRASTTGAAL
                     GRLARYYGLLSKATRYHVQPHGAAVLVRTELFGRRPPGVEVFAVGRTLALLRREAIGE
                     VTAREVWLTQPRGSAKQAVAYEQGFAAPVRYGQAIAGYLLEPGALDRPMRRADPLVGD
                     LLERYAECSMIAATHTPLAARVRSELAVRPPDELAAPTIARALGISERAMRRGLQAEG
                     TSLRDEQGAVLRERAIAALQHRSVEQVAAELGYADAAAFRRAFRRWCGVSPGEYARSA
                     APITGTRRARAG"
     gene            complement(1012..1587)
                     /locus_tag="VGB85_16825"
     CDS             complement(1012..1587)
                     /locus_tag="VGB85_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135752.1"
                     /translation="MHDFDLLHTHRGPRLLALALASLTMIACPIDDETPGQPDASTTD
                     ATSGSSGTTSETTGATTYSLSGTIGRTVDFAPENDGVGTLVIGAFAQCGVGTSLLATA
                     VVPGADFSDPEARVAFTLEGLPPGPVYVGLFLDDNGDLDPMAPRPNEGDLVYGFDPCD
                     GDLDCVELAVAGADVVDVALDLNLLELDVCP"
     gene            1702..2310
                     /locus_tag="VGB85_16830"
     CDS             1702..2310
                     /locus_tag="VGB85_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135753.1"
                     /translation="MNARAPWLLTLLTLIVSIIAETACDAPTRPAPVAPAPVTPAPVA
                     PTPVPPATAAPMQPPPSTAATALVGPATADVLLHGSERATFVVDAKSFIQHSTDPARF
                     AGYHVLRRGRALTDDEHRRLVALLLDEHSYLPIEKEVCFQDEAYGLRVTHGAEVVELL
                     FVFPCDRVNFLRRASADTQRMPGEYIDPVAPQVLAILQAATR"
     gene            complement(2315..2881)
                     /locus_tag="VGB85_16835"
     CDS             complement(2315..2881)
                     /locus_tag="VGB85_16835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEY0135754.1"
                     /translation="MQRSEREKMLAGELYLASDPELVALRRAARTITREYNATTEDEP
                     ARREALLRALFGGVGARIEVEPPFRCDYGGNIRVGDGFYMNFDGVILDCAAVEIGDQV
                     FCGPGVHIYAATHPVRASERVRGPELARPVRIGHRVWLGGRVVVCPGVTIGDDTTIAA
                     GSVVTRDIPARVLAGGNPCRVLRALDGP"
     gene            3200..>3517
                     /locus_tag="VGB85_16840"
     CDS             3200..>3517
                     /locus_tag="VGB85_16840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135755.1"
                     /translation="MILTLRRELPLSLLLLAGCPDSDELATANTPDGTSGSTGGSTTA
                     APVTSGGDSSATSTSTWPGTGTTETTSGIDSMPGSGTTGSETTGSGTTDGTTGSDTTG
                     GADT"
BASE COUNT          505 a         1326 c         1200 g          486 t
ORIGIN      
        1 gacggcacga tcgccgggtg cagtcccccg gtcacatcgc cagcatcgcc gtgcgctccg
       61 tgctcgatgg tctgcgggcg atcggcgccg agccggccca ggtgctgcgg ccgctcgggg
      121 tcgcgtcggc gctcgccgag ggcgacgagc gctggctgcc gcacgcgatg agcgacgcgc
      181 tgtggcgcgt cgcctgtgag cgcatgggac cgatgctgcc gctgcagctc gccgagcacc
      241 tgccgcacga gcgctgcggg cacctcggcg cgatggcggt gcgcgcgagc accaccggcg
      301 cggcgctcgg ccggctggcg cgctactacg ggctgctgtc gaaggcgacg cgctaccacg
      361 tgcagccgca cggggctgcg gtgctggtgc gcaccgagct gttcggccgc cgtccgccgg
      421 gggtcgaggt cttcgcggtc ggccggacgc tcgcgctgct gcgacgcgag gcgatcggcg
      481 aggtcacggc gcgcgaggtg tggctgaccc agccacgcgg ctccgcgaag caggccgtcg
      541 cttatgagca gggcttcgcg gcgccggtgc gctacggcca ggcgatcgcg ggttatctgc
      601 tcgaacctgg ggcgctcgac cggccgatgc gacgcgccga cccgctcgtc ggcgacctgc
      661 tcgagcgcta cgccgagtgc agtatgatcg cggccacgca caccccgctc gccgcgcgcg
      721 tgcggtccga gctggccgta cgaccgcccg acgagctcgc ggcgccgacg atcgcccggg
      781 ccctggggat cagcgaacgg gcgatgcgcc gcggtctgca agccgagggc acctcgctgc
      841 gcgacgagca gggcgccgtg ctgcgcgagc gggcgatcgc cgcgctgcaa caccggtcgg
      901 tcgagcaggt cgccgccgag ctcggctacg cggacgcggc ggcgtttcgc cgggcgtttc
      961 gccgctggtg cggggtctcg cccggcgagt atgcccgcag cgccgcgccg atcacgggca
     1021 cacgtcgagc tcgagcaggt tgaggtcgag cgcgacgtcg acgacatcgg cgccggcgac
     1081 cgccagctcg acgcagtcga ggtcgccgtc gcagggatcg aaaccatata cgaggtcacc
     1141 ctcgttcggc cgcggcgcca tcgggtcgag gtcgccgtta tcgtcgagga acaggccgac
     1201 atacacggga cctggcggca ggccctcgag cgtgaaggcg acgcgcgcct cgggatccga
     1261 gaagtcggcg cccggcacga ccgccgtggc gagcaacgac gtaccgacgc cgcactgcgc
     1321 gaacgcgccg atcaccagag taccgacacc gtcgttctcg ggcgcaaagt cgacggtgcg
     1381 gccgatcgtg ccggagagcg agtaagtcgt cgcgccggtc gtctcgctgg tcgtaccgga
     1441 gctgccggag gtcgcgtccg tggtgctggc gtccggttgg ccgggcgtct cgtcgtcgat
     1501 cgggcaggcg atcatggtga ggctggcgag cgcgagggcg agcagccggg ggccgcggtg
     1561 ggtgtggagc aggtcgaagt catgcatgcc acgaatgtgc gtggcgcggc ccgccgccgg
     1621 caggacagtt ccggccaatc tcgcggccga ttcggcctca ccgcgagcca cggcgcagcg
     1681 gatccatgcg acactacccg cgtgaacgct cgcgccccgt ggctgctcac gctgctcacg
     1741 ctgatcgtct cgatcatcgc cgaaaccgcc tgcgacgccc ctacccggcc tgctcccgtc
     1801 gcgccggctc ccgtcacgcc cgctcccgtc gcgcccacgc ccgtcccccc tgccaccgcc
     1861 gcgccgatgc aaccaccgcc ctcgaccgcc gccaccgccc tcgtcggccc tgccaccgcc
     1921 gatgtgctgc tccacggcag cgagcgggcg accttcgtgg tcgacgcgaa gtcgttcata
     1981 caacactcga ccgacccggc ccgcttcgcc ggatatcacg tgctgcgtcg cggtcgcgcg
     2041 ctgaccgacg acgagcaccg ccgcctggtc gcgctcttgc tcgacgagca cagctacctc
     2101 ccgatcgaga aggaggtctg cttccaggac gaggcctacg gcctgcgcgt cactcacggc
     2161 gccgaggtcg tcgagctgct gttcgtgttc ccctgcgacc gcgtcaactt cctgcgccgc
     2221 gccagcgccg acacgcagcg catgcccggc gaatacatcg atccggtcgc cccgcaggtg
     2281 ctcgccatct tgcaggccgc cacacgctga gggatcacgg cccgtcgagc gcccgcagca
     2341 ctcgacacgg gttcccgccg gcgagcacgc gcgccgggat gtcacgcgtc accacgctgc
     2401 ccgcggcgat cgtcgtgtcg tcgccgatcg tcacgcccgg gcacacgacg acgcgccccc
     2461 cgagccacac gcgatgcccg atccggaccg gacgcgccag ctccgggccg cgcacgcgct
     2521 cgctcgcgcg caccgggtgc gtggcggcat agatatgcac gcctggcccg cagaacacct
     2581 gatcaccgat ctcgaccgcc gcgcagtcga ggatcacgcc gtcgaagttc atataaaaac
     2641 catcgccgac gcggatgttg ccgccgtagt cgcaacgaaa cggcggctcg acctcgatcc
     2701 gtgccccgac tccgccgaac agcgcgcgca gcagcgcctc gcggcgcgcc ggctcgtcct
     2761 cggtcgtcgc gttgtactcg cgggtgatcg tccgggccgc tcgccgcagc gcgaccagct
     2821 cgggatccga ggcgaggtag agctcgccgg ccaacatctt ctcgcgctcg ctccgctgca
     2881 cgccgtgagc atcgcatgaa gcgcgcccac gcgcacgcgc tgcgacccgc tcgcggtatc
     2941 ttccaccccg tgacgagccg atcgccgagc cgatcgagcc ggaccgcacc ggcgcgacct
     3001 gctatttgct gttacgacta tcgggacttg cgcgcgagga cgcaattaca cgcatccgcc
     3061 tcgcccgcgc cctcaccgcc gaggagctgt ggcctcgatc tcacggatcg ttcgttcggg
     3121 tatccgcctg gtgaatttat ctccaggccc gaacatcatg cattcccttt cggtcgacaa
     3181 accatgatgt catggtccga tgatccttac gttgcgccgc gagctcccgc tctcgcttct
     3241 cctgctcgcc ggctgtccgg actccgacga gctcgcgacc gcgaatacgc cggacggcac
     3301 gagcggctcg accggaggct cgaccactgc agcgccggtg acctcgggag gcgactcgtc
     3361 cgcgacgagc acctcgacct ggccgggcac cggcaccacc gaaaccacca gcggcatcga
     3421 tagcatgccc ggctccggga ccacgggcag cgaaaccacg gggtcgggca ctaccgacgg
     3481 gaccacggga tccgacacca ccggcggcgc cgacacg
//
LOCUS       DASWNJ010000992         2077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_53868, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000992 DASWNJ010000000
VERSION     DASWNJ010000992.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53868"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="VGB85_16845"
     CDS             <1..1070
                     /locus_tag="VGB85_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135756.1"
                     /translation="EEGGNLVEVAEPEVFRWYSALQASEVELTREDLDVGFAPVHRLT
                     IETDSGQSLTLACGAGGSVALACARDEGPPLRVLGTPPELAFSRETFADRRLLSFASG
                     ELRSLELLPGPASQGVRQSVRLDLGVWRLDAPAHPDGVAVLDEVRLEGMLAALQGLRA
                     EAWTPVPATAPQRTLRVERGHGGTGDDGSLSVDLHLPRPELPAICVGHVPGQPQAARL
                     SESTCAALQDDLLYNDPLRFWLTQARSIQLTDRSDADERVAMLRREGPATEEGASWSV
                     ESGEPVLLAELPRWVAFRSAGLRSGEPRGATAIEAKIWRNAAAAIRVDLGPIVDGAPA
                     WVRLAGAPWYYLAGPPEPESP"
     gene            1201..>2077
                     /locus_tag="VGB85_16850"
     CDS             1201..>2077
                     /locus_tag="VGB85_16850"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEY0135757.1"
                     /translation="MPVIACGESVELRPVVTMPGPGPQRRDGDAVVRNDECVACHEDI
                     AAEWQGSLHREAYTGREFQHALRSEPLPVCRGCHAPEADLRRPQPALTAIGVACVTCH
                     VPVGDEVLSAREQGAPMTAPHPVRRDADFAGSGACAGCHEFTFSDRRPVPEYMQTTIR
                     EHAASPLRDRSCADCHMPRANDGHRTHGFAASRDAAWMRSVVTVTAVRPAADRVEVTL
                     DLREDRVGHAFPTGDLLRRLAITVEVARAGPRRAQRRYLARHWTSTRFAGGPSVRSEA
                     SDDRLGVGEDPRTLVF"
BASE COUNT          249 a          749 c          791 g          288 t
ORIGIN      
        1 tcgaggaggg cggcaacctc gtcgaggtcg ccgagcccga ggtgttccgc tggtacagcg
       61 cgctgcaggc gagcgaggtc gagctgacgc gcgaggacct cgacgtcggc tttgcgcccg
      121 tgcatcgcct gacgatcgag accgacagcg gacagagcct gacgctcgcg tgtggggcgg
      181 gcggctcggt cgcgctggca tgcgcgcgtg acgagggccc gccgctgcgc gtgctcggca
      241 cgccgcccga gctggccttc tcgcgcgaga ccttcgccga ccgccgcctg ctcagcttcg
      301 cgtcgggcga gctgcgcagc ctcgagctgc tgccggggcc ggcgagccag ggggtgcgcc
      361 agagcgtgcg cctcgacctc ggcgtgtggc gcctcgacgc gccggctcac cccgacggcg
      421 tcgccgtgct cgacgaggtg cgcctcgagg gcatgctcgc cgcgctgcag ggcctgcgcg
      481 ccgaggcgtg gacgccggtg cccgcgacgg cgccgcagcg cacgctgcgg gtcgaacgcg
      541 gccacggtgg gacgggcgac gacggctcgc tcagcgtcga cctgcacctg ccgcggcccg
      601 agctgccggc gatctgcgtc gggcacgtgc cggggcagcc gcaggcggcc cgcctgtccg
      661 aaagtacatg tgctgcgctg caggacgacc tgctgtacaa cgacccgctg cggttctggc
      721 tgacccaggc gcgcagcatc cagctgaccg accgcagcga cgccgacgag cgcgtggcga
      781 tgctgcgccg cgagggcccg gcgacggagg agggcgcgtc gtggtcggtc gagtcgggcg
      841 aaccggtgct gctggccgag ctgccgcgct gggtcgcgtt tcgcagcgcg ggcctgcgca
      901 gcggcgagcc gcgcggcgcg accgcgatcg aggccaagat ctggcgcaac gccgcggcgg
      961 cgatccgcgt ggacctcggc ccgatcgtcg acggcgcccc ggcctgggtc cgcctcgccg
     1021 gggctccctg gtactacctc gcaggcccgc ccgagcccga gtcgccctga cggggcgcgc
     1081 tcgcacgcgg cacgaagggc gccaggacgg cgttcgcgcg cgaagtcgcc gtcgcgtgac
     1141 gattcgtgcg attggttgtt ttgcgtggtt tcttggtctt cgaggtgtgt tttcgcgctg
     1201 atgccggtga tcgcctgcgg cgagtccgtg gagctccgac cggtcgtgac gatgcccggg
     1261 cctgggccgc agcggcgtga cggggacgcc gtcgtgcgca acgacgagtg tgtcgcctgc
     1321 cacgaggaca tcgctgccga gtggcagggg tcgttgcacc gcgaggcgta caccggccgc
     1381 gaatttcagc atgcgttgcg cagcgagccg ttgccggtgt gtcgcgggtg tcatgcgccc
     1441 gaggcggacc tgcgtcgtcc acagcccgcg ctcacggcga tcggtgtcgc ctgcgtgaca
     1501 tgccacgtgc cggtcggcga cgaagtcctg tcggcgcgcg agcagggggc gccaatgacg
     1561 gcgccgcacc cggtgcgccg ggacgctgac ttcgcgggat cgggcgcgtg cgccggctgt
     1621 cacgagttca ccttctcgga tcgccgacct gtccccgagt acatgcagac cacgatccgc
     1681 gagcacgccg cgtcgcccct gcgcgaccgc agctgcgccg actgtcacat gccacgcgcg
     1741 aacgacgggc atcgcacgca cggcttcgcg gcctcgcggg acgccgcgtg gatgcgcagc
     1801 gtcgtcaccg tgaccgctgt tcgacccgcg gcggaccgcg tcgaggtgac gctcgacctg
     1861 cgcgaggatc gcgtcgggca tgcgttcccg accggcgacc tgctgcgtcg cctggcgatc
     1921 accgtcgagg tcgcccgcgc gggcccccgc cgagcgcagc ggcggtacct cgcgcgtcac
     1981 tggacgagca cgcgcttcgc cggcggcccc tccgtgcgca gcgaggcgag cgacgaccgc
     2041 ctcggcgtcg gcgaagaccc gcgcacgctc gtgttcg
//
LOCUS       DASWNJ010000993         3476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_549009, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000993 DASWNJ010000000
VERSION     DASWNJ010000993.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549009"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="VGB85_16855"
     CDS             complement(<1..1168)
                     /locus_tag="VGB85_16855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135758.1"
                     /translation="MHARQLVCMGLAGCFAVGCFSPSGSEDTGGASSEAGASTSVVTT
                     DSVPTTGAGASGTTDEGGATSMQGGSGDSSTGDTATGEPSDGCGDPPFIATEELLQTI
                     RDDLGGLLPEDWSFTRYLTLVHLHNAGVCEAEIEVERRALTKLLNSLSRASLPVVPEP
                     VDAGRLVFRIDLRDYTWSTTDEMPPSLSEPSVYYPEVSELFKVYDDRWELLVDQNPYR
                     LRHVGVQADEIEVLTGTASPILRADALVDAAVRPPLYYDALGMPVTRDALETVLELDL
                     EAALAMERSQDSEHVARAALRSSPLTGAVRALERQDFPANPNRAIWQTFDFVPRNTDV
                     FEQPFDFTADGSEVLFNLPNGLHAYLSLDSVGNRRKSVPPDIFTDPAQPDAVARV"
     gene            complement(1384..2001)
                     /locus_tag="VGB85_16860"
     CDS             complement(1384..2001)
                     /locus_tag="VGB85_16860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135759.1"
                     /translation="MQPSVVLRLYPRRTPVVMAVAAWGILATTGLLISAIARLTPMAL
                     EPLQSHEMNGLQWALYVGWAIFNGYVEGYRGFQKGYVPRVVARAFWLGEHPRPLFVLL
                     APFFCSGLFHATRKRLIISWCLLIGIIGIVQLVRLLEQPWRGIVDAGVVVGLAYGTGY
                     LVVHFAAVLLGRPITVTPQVPSVEPVGDDPSLSASSVAVPVDLRR"
     gene            complement(2060..3298)
                     /locus_tag="VGB85_16865"
     CDS             complement(2060..3298)
                     /locus_tag="VGB85_16865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0135760.1"
                     /translation="MQHSVQRASSKLTSARSVTIGARATAETGDPLATYFKDVAQHPV
                     MSREEEMAVANTIASQRQALWRALLGHPRAVAIACERARALLSGEALPAAELTAIEAA
                     VAAMQGRVTASQRKAFAEAREALAVALAEQDVDGIVTDATLAEVERAAGNVSPKAEAA
                     FAALRVEARAARRTLAASKERFVQANLRLVVMVARRFNHGRLALHDLIQDGNIGLMKA
                     VDRFDPRRGFRFSTYATWWIRHSISRALADTGRAVRLPVHMIEAQGRVARVRREFEAL
                     HGREPTDTELATASGLSLERIQRMRWSLIEGPVSLDAPVSDGPGRTLQDLLADADAPP
                     APELIDSELLHRRLREAFAALPAMEAAVLRERMGMDDEPEQTLREIGERHALSRERIR
                     QIQEQGLARLRREFRRHDLM"
BASE COUNT          515 a         1297 c         1183 g          481 t
ORIGIN      
        1 cgacccgggc gacggcgtcc ggctgcgcgg ggtcggtgaa gatgtccggc ggcacgctct
       61 tgcggcggtt gccgacgctg tcgaggctca ggtacgcgtg gagcccgttg ggcaggttga
      121 acagcacctc gctgccgtcg gccgtgaagt cgaagggctg ctcgaagacg tcggtgtttc
      181 gtgggacgaa gtcgaaggtc tgccagatcg cgcggttcgg gttggcgggg aagtcctggc
      241 gctcgagggc ccgcaccgcg ccggtcaggg gcgagctccg gagggccgcg cgcgccacat
      301 gctcggagtc ctggctgcgc tccatcgcca gcgccgcctc gaggtcgagc tccagcaccg
      361 tctccagcgc gtcacgcgtc accggcatcc cgagcgcgtc gtaatacaac ggaggccgca
      421 ccgccgcgtc gacgagcgcg tcggcgcgaa ggatcgggct cgccgtcccg gtcaggacct
      481 cgatctcgtc ggcctgcacg ccgacatgcc ggagccggta cgggttctgg tcgacgagca
      541 gctcccagcg gtcgtcgtag accttgaaca gctcgctgac ctccgggtag tagaccgagg
      601 gctccgacag cgagggcggc atctcgtcgg tcgtgctcca cgtgtaatcg cgcaggtcga
      661 tgcgaaacac cagccgaccc gcgtcgaccg gctcgggcac gacggggagc gacgcgcgcg
      721 agaggctgtt gagcagcttc gtgagcgccc ggcgctcgac ctcgatctcg gcctcgcaca
      781 ccccggcgtt gtgcaggtgc acgagcgtca ggtagcgcgt gaacgaccag tcctccggca
      841 acagaccgcc gaggtcgtcg cggatcgtct gcagcagctc ctccgtggcg atgaacggcg
      901 gatcaccaca accgtccgaa ggctcgcccg tcgcggtgtc ccccgtcgag ctgtcacccg
      961 acccaccctg catcgaggtc gccccaccct catccgtcgt gccgctggcg ccggcgccgg
     1021 tggtggggac cgagtccgtc gtgaccaccg aagtgctcgc ccccgcctcg ctgctcgccc
     1081 cacccgtgtc ctcactcccc gacggcgaga agcagccgac cgcgaagcac cccgcgagcc
     1141 ccatgcaaac cagctggcgt gcgtgcaccg ttcgaacgtc gtcagacata caacccactc
     1201 cgttgtaccc gagcccgccg cccgccgccc atgaccacat acccagcgcc aggtccgccg
     1261 gaccgcggcc gctcctcatt ccgagcatgc gcctgccgcg atcacgaccg atcaccgcgc
     1321 gtacacgagc ggggcgccga cgatgtccgc ccggggttgg gggccgccga gcggcccgcc
     1381 cgatcaccgc cgcagatcga cgggcaccgc gaccgacgag gccgacagcg acggatcgtc
     1441 gcccaccggc tccaccgagg gcacctgcgg cgtcaccgta atcggccgcc cgagcagcac
     1501 cgccgcgaag tgcaccacca aataacctgt cccataggcc agcccgacca cgacgcccgc
     1561 gtcgacgatc ccccgccatg gttgctcgag cagacgcacc aactgcacga tgccgatgat
     1621 gccgatcagc aggcaccacg agatgatcag ccgcttgcgg gtcgcgtgga acagccccga
     1681 gcagaagaac ggcgccagca gcacgaacag gggtcgcggg tgctcgccga gccagaacgc
     1741 gcgggcgacc acgcggggca cgtagccctt ctggaacccg cggtagccct cgacgtagcc
     1801 gttgaagatc gcccagccga cgtacagcgc ccactgcagc ccgttcatct cgtgcgactg
     1861 cagcggctcg agggccatcg gtgtcaggcg ggcgatcgcg ctgatcagca ggcccgtggt
     1921 cgccaggatg ccccaggccg cgaccgccat gacgacgggc gtgcggcgag ggtagaggcg
     1981 aaggacgacg ctcggctgca aggcggcatg tctcgcacga tcggccgccc cgggcaacct
     2041 acctggctca catcagggct cacatcaggt catgccggcg aaactcgcgg cgcagccgcg
     2101 cgaggccctg ctcctggatc tgccggatgc gctcgcgcga cagcgcgtgg cgctcgccga
     2161 tctcccgcag ggtctgctcg ggctcgtcgt ccatgcccat gcgctcgcgc agcaccgcgg
     2221 cctccatcgc cggcagggcc gcgaacgcct cgcgcaggcg gcggtgcagc agctcgctgt
     2281 cgatcagctc cggcgcgggc ggggcgtcgg cgtcggcgag caggtcttgc agcgtgcggc
     2341 cgggcccgtc gctgaccggc gcgtcgaggc tgacggggcc ttcgatcagc gaccagcgca
     2401 tccgctggat ccgctcgagg ctgaggccgg acgccgtcgc cagctccgtg tcggtcggct
     2461 cgcggccgtg cagcgcctcg aattcgcggc gcacgcgggc gacgcggcct tgcgcctcga
     2521 tcatgtgcac cggcagccgc accgcgcggc ccgtgtccgc cagcgccctg ctgatcgagt
     2581 gccggatcca ccacgtcgcg tacgtcgaga agcggaaccc gcggcgcggg tcgaagcggt
     2641 cgaccgcctt catcaggccg atgttgccgt cctggatcag gtcgtgcagc gccaggcggc
     2701 cgtggttgaa gcgccgggcg accatcacca ccagccgcag gttcgcctgc acgaagcgct
     2761 ccttgctcgc cgccagcgtg cgccgggccg cgcgggcctc gacgcgcagc gccgcgaacg
     2821 ctgcctcggc cttcgggctc acgttgcccg ccgcgcgctc gacctccgcg agcgtcgcgt
     2881 cggtgacgat gccgtcgacg tcctgctcgg cgagcgcgac cgccagcgcc tcgcgggcct
     2941 cggcgaaggc cttgcgctgg ctcgccgtga cgcggccctg catcgccgcg acggcggcct
     3001 cgatcgccgt cagctccgcg gcgggcagcg cctcccccga gagcagcgcc cgcgcccgct
     3061 cgcacgcgat cgccacggcg cgcgggtggc cgagcagcgc ccgccacagc gcctggcgct
     3121 ggctggcgat cgtgttggcg accgccatct cctcctcgcg gctcatcacc gggtgctggg
     3181 cgacatcttt gaaataagtc gccagcgggt cgccggtctc cgcggtcgcg cgtgcgccga
     3241 tggtgacaga ccgggccgag gtgagcttcg aggaggcgcg ctggacagag tgctgcatgg
     3301 tcgggttccc gtgcctcaac actggaacct tgaacagact ttgtcaaccg tgaatctaaa
     3361 gtttgttcat ggatggccgc cgtccgcgcc gaacagcgcc accctgcgcc ccatcgcggg
     3421 catactgtcc cacctccgaa cgcatttgtc caacgctcgt caatccttcg ggccgg
//
LOCUS       DASWNJ010000994         1677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_330400, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000994 DASWNJ010000000
VERSION     DASWNJ010000994.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330400"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /locus_tag="VGB85_16870"
     CDS             complement(<1..1217)
                     /locus_tag="VGB85_16870"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0135761.1"
                     /translation="MSNLVGTKLDKYEIQSEVGHGGMAVVYRGLDTVLNREVAVKVLH
                     PHMAAREESRARLRREALTVARLRHENILEIYDYSGENAVESFLVTEFIHGMTLREWL
                     DTRWRPRPALAAMVVHRLCVALGHAHKIGIVHRDIKPENVMIRSDGCLKLMDFGIAQI
                     IDHQKLTMTGQLLGSPAYMAPELISGKPIDARTDLFAVGIMLYQLATGALPFSGRNPH
                     EVLSRIADAEYPKACTVCPLVDDELEAIIAKALAREPDLRYQSAEDFARELERYLEEV
                     GIPASQEEVIGYFIDCDRYVDQLDKRVCTTLMTRAETAARAGHSARAIRLLGRVLELE
                     KDQKQAKSMLSRLRTRERTVKRLMVGAAAVGLTALGSGAGVLWYMTPPDIENLPRADE
                     RVVPDLRPTPSTP"
     gene            1474..>1677
                     /locus_tag="VGB85_16875"
     CDS             1474..>1677
                     /locus_tag="VGB85_16875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135762.1"
                     /translation="MASVAFVPAPAEGLNYPNVIQMFVARARRTPDLPALRFKRHGIW
                     RALTWRDWLRASEAIAAALIARLG"
BASE COUNT          231 a          582 c          591 g          273 t
ORIGIN      
        1 aacggcgtcg acggggtcgg gcgcagatcg ggcacgacgc gctcgtcggc gcgcggcagg
       61 ttctcgatgt ccggcggggt catgtaccac agcacgccgg cgccgctgcc gagcgcggtc
      121 agcccgaccg ccgcggcgcc gaccatcagc cgcttgacgg tgcgctcgcg cgtccgcagg
      181 cgcgacagca tcgacttggc ctgcttctga tccttctcca gctcgagcac gcggccgagc
      241 aggcggatcg cgcgggcgct gtgaccggcg cgcgcggccg tctcggcgcg cgtcatcagc
      301 gtggtgcaca cgcgcttgtc gagctgatcg acgtagcggt cgcagtcgat gaagtagccg
      361 atgacctcct cctgcgaggc cgggatcccg acctcctcga ggtagcgctc gagctcgcgg
      421 gcgaagtcct cggcgctctg gtagcgaaga tcgggctcgc gggcgagggc cttggcgatg
      481 atcgcctcga gctcgtcgtc gaccaacgga cacaccgtgc acgccttggg gtattcggcg
      541 tcggcgatcc ggctgaggac ctcgtgcggg ttgcggccgg agaaggggag ggcgccggtc
      601 gccagctggt agagcatgat gccgaccgcg aacaggtcgg tgcgcgcgtc gatcggcttg
      661 ccgctgatca gctccggcgc catgtaggcc gggctgccga gcagctggcc ggtcatcgtc
      721 agcttctgat gatcgatgat ctgggcgatg ccgaagtcca tcagcttgag gcagccgtcg
      781 ctccggatca tcacgttctc cggcttgatg tcgcggtgaa cgatgccgat cttgtgcgca
      841 tgcccgagcg cgacgcacag ccggtgcacg accatcgccg ccagcgcggg ccgcggacgc
      901 cagcgcgtgt cgagccactc gcgcagcgtc atcccgtgga tgaactcggt gacgaggaag
      961 ctctcgaccg cgttctcacc cgaataatcg taaatctcga ggatgttctc gtggcgcagg
     1021 cgggccaccg tgagcgcctc gcgccgcagc ctcgcccgcg actcctcgcg cgccgccatg
     1081 tgtgggtgca agaccttgac cgcgacctcg cggttgagca ccgtgtcgag cccgcgatac
     1141 acgacagcca tcccgccgtg gccgacctcg ctctggatct cgtacttgtc gagcttggtt
     1201 ccgacgaggt tactcatcgc agggcactct tcctctacta caaaacggca gcccccggcg
     1261 ggcgcggcca cgcggcgcgg gaggtagaca ccggacccgc ctcactttct gtgggtcctc
     1321 gctgtgggtc ctcgttcgcc acggtcgccg gtcgcgaggg tcgcgagccc ccggggcagt
     1381 gggcgtccac gcgcttgccg gggtcggccg aggggggccg ggtcggccga cctcgcggcc
     1441 gacggacctt cgtggtactg cgaacaggcg tccatggcct ccgtcgcttt cgtgcccgcc
     1501 ccggccgagg ggttgaacta ccccaacgtg atccagatgt tcgtggcgcg cgcccggcgc
     1561 acgccggacc ttccggcgct gcgcttcaag cgacacggga tctggcgagc cctgacctgg
     1621 cgcgactggc tccgggccag cgaggcgatc gcggcggcct tgatcgcccg cctcggg
//
LOCUS       DASWNJ010000995         2559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_195596, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000995 DASWNJ010000000
VERSION     DASWNJ010000995.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195596"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            663..>2559
                     /locus_tag="VGB85_16880"
     CDS             663..>2559
                     /locus_tag="VGB85_16880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135763.1"
                     /translation="MQVPIAVPPIEPAGGPVLPPAPTTADIDVDFDDDEAEPAAPVRA
                     EPTRSPPASAAAKPTAPIPAAAPPPGTPVKSAAPASASQSKPALSAAALFTRAPEEDS
                     VPGSGGGMPSRTPPPATSKTMPIDLDPEMIPAPKGPPRPPPGPPPTGGRGTVEPTRRQ
                     ITEPSPVAAPVAAPVAAPVDPRAATTPSEPRPTIAEPRPGSAEPRPTTTPAAPRRPAT
                     DPVGASAVPPAEPRRPVTEPAAGRPAFSPPGAAPAPGDPNRPAVASGTRVMNRVPPPG
                     GMRPAVPGAPGSLAGPPATHTSSVASLPTMVSTGPADRSAGTGPSGQATRVRDTPVSM
                     SALTEVTSDPAQARTRYASEAQRPAAAMPHSPSGPVTRHISSPSVTAENPFLRYAQTQ
                     IQAWEVELATQPDLFRAARLNYEIARLLEYPLGDLNQAATYYLRAHGLLPEYLPILRG
                     ARRVLMAQRNFQRALPLFDAEIRLTSDPRRKAALMYAKGSLLADVLHNETDARAAYAA
                     ARELDPGNVSILKALEQIDARVGAWDPLEKELAQIASAVEKDPRHRSALVARRAHLFE
                     VRQKNVGAAVELLETALDLDPQAPGAFAALKRMHHGQGRWRELIGVLDREAQQLSDPD
                     LRAMAFYR"
BASE COUNT          339 a         1077 c          839 g          304 t
ORIGIN      
        1 tcgtcgcgcg acagcgccgg cgtcagcagc cgcagcgcga cctcccgcga gaacgcgagg
       61 tcctcggcgc ggtacaggat ccccgcgctg ccggcgtgga tgcgcccgag caggcgatag
      121 cggttggcga tcggcgcgtc gtgctcgccg gtcaccgtca gcggcgacgg ctccgccacg
      181 gcgtccgcgt cccgcgtctc gcgcggtccc ggtcggtcca ggagatgtag ccgttcgtgg
      241 ctgggtgtcg ctcccatggc gacgcaacca tagcgaagcc acgccgcggt gccgagcgtc
      301 ttcggccgtg gctgtgacga ccatcccggt cccttgcatc cgcaagatct cgcgtcgtgg
      361 cccgtcgcac ccgcgaggag cgcggaagca ccccgccgga tcccggccgc ggacccgccg
      421 tcgcgcacgt atttcgcccg gcgatcaggc atactcccgg ctccacgaga gaaggacggc
      481 gagtgcctca gcgcgacgat cagggtccga agtccccacc ttctgcgggt acgccgactt
      541 ccgcgagctc accaggcggg tccggatcgg gccttgccgg ccttcctggc caggatgacg
      601 agttcccgcg cgacgcggcc gatctcttcg acgcgatcat cgtcgacgag ccgcgccgcc
      661 ccgtgcaggt gccgatcgcc gttccgccga tcgagcccgc gggcggaccg gtgctgccgc
      721 cggccccgac caccgccgac atcgacgtcg attttgatga cgacgaggcc gagcccgccg
      781 ccccggttcg cgccgagccc acgcgctcgc cgccggcctc cgccgccgcc aaaccaaccg
      841 caccgatccc cgctgcggcc ccaccgccgg gcaccccggt gaagtccgcg gcgcccgcca
      901 gcgcctccca gagcaagccc gcgctctcgg ccgccgcgct gttcactcgc gcgcccgagg
      961 aggacagcgt ccccgggagc ggcggcggca tgccatcgcg cacccccccg cccgcgacct
     1021 ccaagacgat gccgatcgat ctcgacccgg agatgatccc ggcgcccaag ggcccgccca
     1081 ggccgccccc gggtccgccc ccgaccggcg gtcggggaac ggtcgagccg acgcgccggc
     1141 agatcaccga gccatccccg gtggccgccc ccgtggccgc ccccgtggcc gcccccgtcg
     1201 atccgcgcgc cgcgaccacc ccctccgagc cgcgcccgac gatcgccgag ccgcgcccgg
     1261 gcagcgccga gccgcgcccg acgaccaccc ccgccgcgcc gcgtcgcccc gcgaccgatc
     1321 ccgtcggtgc ctctgccgta cctccggcgg agccgcgccg ccccgtcacc gagccggccg
     1381 cgggccgccc cgcgttctcg ccgccgggcg ccgcgccggc cccgggcgac ccgaaccgtc
     1441 ccgccgtcgc cagcggcacc cgcgtcatga accgcgtgcc gccccccggc ggcatgcgcc
     1501 cggccgtacc cggcgctccg ggctcgctcg caggcccacc ggcgacgcat acctcctccg
     1561 tcgcctcgct accgacgatg gtgtcaactg gcccggcgga caggtccgct gggacaggtc
     1621 cttcgggaca ggccacgcgg gtccgcgaca cgccggtctc gatgtccgcg ctgaccgagg
     1681 tcaccagcga tccggcgcag gcgcgcacgc gttacgcgag cgaggcccag cgtccggccg
     1741 cggcgatgcc ccacagcccc tcgggcccgg tgacgcggca catcagtagc ccttcggtga
     1801 ccgccgagaa cccgttcctg cgctacgcgc agacccagat ccaggcgtgg gaggtcgagc
     1861 tcgcgaccca gccggacctg ttccgcgccg cacgtctcaa ctacgagatc gcccgcctac
     1921 tcgagtaccc gctcggcgac ctcaaccagg ccgcgaccta ctacctgcgc gcgcatggtc
     1981 ttctgcccga gtacctgccg atcctgcgcg gtgcccggcg cgtgctgatg gcccagcgca
     2041 actttcaacg cgcgctgccc ctgttcgacg ccgagatccg cctgacctcg gacccgcgcc
     2101 gcaaggccgc cctgatgtac gccaagggca gcctgctggc cgacgtcctg cacaacgaga
     2161 ccgacgcgcg ggccgcctac gccgccgccc gcgagctcga ccccggcaac gtctcgatcc
     2221 tcaaggcgct cgagcagatc gacgcccgcg tcggcgcctg ggatccgctg gagaaggagc
     2281 tcgcgcagat cgccagcgcc gtcgagaagg acccgcgcca ccgctcggcc ctggtggccc
     2341 gccgcgcgca cctcttcgag gtccgccaga agaacgtcgg cgccgcggtc gagctgctgg
     2401 agaccgccct cgacctcgac ccacaggccc ctggcgcgtt cgctgcgctc aagcgcatgc
     2461 accacgggca gggccgctgg cgcgagctga tcggcgtgct cgaccgcgag gcccagcagc
     2521 tgtccgaccc cgatctgcgg gcgatggcgt tctatcgga
//
LOCUS       DASWNJ010000996         1771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_266271, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000996 DASWNJ010000000
VERSION     DASWNJ010000996.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266271"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="VGB85_16885"
     CDS             complement(<1..824)
                     /locus_tag="VGB85_16885"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0135764.1"
                     /translation="MIRVTIIAQGAEEASETKEFTSGQISIGRLGKNDLVLVSSRVSS
                     MHARALVAEHGVTVIDNNSTNGTFVNGTMVRGPVTVEPEDTVEIGSFTLHFENITAAE
                     EPLEDDGSGIAAEEFDELVAAEPSEFGEPDLLPLETAPSDAFGEPESLLLEPEPEPEP
                     ERREATPFDMRSPLHTGTVPDSYLPRSRGAGRAPAEGPTGLAGAFESTVVALAPSPMV
                     ERTEARALACARDAVELCCPEMDPRQRRQWSEWIAQEVVGVGPLTDLLADASVSEVL"
     gene            909..1610
                     /locus_tag="VGB85_16890"
     CDS             909..1610
                     /locus_tag="VGB85_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135765.1"
                     /translation="MSQVKGHSKLRADQPPLVPIRATLERVLRSFVGPALVTLVVMGS
                     GCARRLDLTPAELARIEGPDGDLKLLRVFPKKKLISLYAEEQVQQSYEVGKRKVTERG
                     AYRPFKRIIGRKTPGKVIARAEQNGMPLLWITFDRGCADTTCAYGFVLTELGLYQLMQ
                     VPFKEKFQVPVNYRRNTFKRNTLKKTKQKSLSEANDVFAVTRKSGKVLTIDLQIRKDT
                     YRPTRADVDRAGGAD"
BASE COUNT          276 a          617 c          607 g          271 t
ORIGIN      
        1 agcacctcgg agacggaggc gtcggcgagc aggtcggtca gcggtccgac gccgacgacc
       61 tcctgggcga tccactcgct ccactgccgg cgctggcgcg gatccatctc cgggcagcac
      121 agctcgacgg cgtcgcgcgc acaagcgagc gcacgtgcct cggtgcgctc gaccatcggc
      181 gagggcgcga gcgcgacgac ggtcgactcg aacgcgccgg cgagcccggt cggcccctcg
      241 gcgggggcgc gcccggcccc gcgcgatcgc ggcaggtacg agtccggcac ggtgccggtg
      301 tgcagcggcg accgcatgtc gaacggggtc gcctcgcggc gctcgggctc cggctccggc
      361 tccggctcga ggagcaggct ctcgggctcc ccgaacgcgt cgctgggggc cgtctccagc
      421 ggcagcaggt cgggctcacc gaactccgag ggctccgcgg cgacgagctc gtcgaactcc
      481 tcggccgcga tcccggagcc gtcgtcctcg agcggctcct cggcggcggt gatgttctcg
      541 aagtgcagcg tgaagctgcc gatctcgacg gtgtcctcgg gctcgacggt gaccgggccg
      601 cggaccatcg ttccgttgac gaaggtcccg ttggtcgagt tgttgtcgat gaccgtgacg
      661 ccgtgctcgg ccacgagcgc gcgcgcgtgc atgctcgaga cccggctgct gaccaggacg
      721 aggtcgtttt tccccaggcg gccgatcgag atctggccgc tcgtgaactc cttggtctcc
      781 gacgcctctt ccgccccctg ggcaatgatc gtaacgcgta tcatcgatat tgctcccgaa
      841 catccgcctc gccgcgcccg gcgaacattg acttggatag cgtcgaggcg taagcacgaa
      901 tggtagccat gagtcaggtg aaagggcaca gcaaactgag ggccgatcag cccccccttg
      961 tccccatccg cgccacgctc gagcgggtcc tacgctcgtt cgtcgggccc gcgctggtga
     1021 cgctggtcgt gatggggagc gggtgcgctc gccggctcga tctcacgccc gcggagctgg
     1081 cgcgcatcga gggccccgac ggggatctca agctgctgcg cgtgttcccc aagaagaagc
     1141 tcatctcgct ctacgccgag gagcaggtcc agcagagcta cgaggtcggc aagcgcaagg
     1201 tcaccgagcg cggcgcttat cgccccttca agcggatcat cggccgcaag accccgggca
     1261 aggtgatcgc gcgcgccgag cagaacggca tgcccctgct gtggatcacg ttcgaccgcg
     1321 gctgcgcgga cacgacctgc gcctacggct tcgtgctgac cgagctgggc ctgtatcagc
     1381 tgatgcaggt gccgttcaaa gagaagttcc aggtgccggt caactaccgg cgcaacacct
     1441 tcaagcgcaa cacgctcaag aagaccaagc agaagtcgct gtcggaggcc aacgacgtgt
     1501 tcgccgtgac ccgcaagtcc ggcaaggtcc tcacgatcga cctccagatc cgcaaggaca
     1561 cctaccggcc gacgcgcgcc gatgtcgatc gggccggcgg cgccgactga ggtcgcgtga
     1621 ggccgcggac gcggcgcgat gcggcgagtt gccgcgtcgc gctgcgcatc acttcaccac
     1681 cccatcacgt cacggcgagc tcggcttgcc gatgcggtcc cacgggctcg gcgtggtgtg
     1741 cagggcgccg tccttgaatt cgagcacgcg t
//
LOCUS       DASWNJ010000997         6247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_123385, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000997 DASWNJ010000000
VERSION     DASWNJ010000997.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123385"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3478)
                     /locus_tag="VGB85_16895"
     CDS             complement(<1..3478)
                     /locus_tag="VGB85_16895"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0135766.1"
                     /translation="MILHALPWQRREHHPPAHVRTLVDLLLHRAAATPDLVIFRHLVD
                     GDDDEQTLTLAALLTQARAIAVTLSRHLPPGARVLLPHPPGLEFAAALLGCMFAGLVG
                     VPVPPPDAGRRGPALRRLLSICADAEVSAIFTVPAVLAAGDELRAALPAIPWFTTDDL
                     DLALADEWTPPALAADTVAYLQYTSGSTADPKGVVIDHANLLHNCGALNEVYALDPTS
                     VMVYWVPTYHDLGLVYGVALPLYIGFTAISMPPAAFLARPLRWLRAITRYRGTHSVAP
                     NFAYELVVARTRPHECTDLDLTSWRHALNGAEPIRQSAEAAFLAAFAPYGLDPDALSH
                     SYGMSEATAELTSEPHPHRGIFLKVAADALERGRVALADDTTRTRVVAGCGVPTGDTE
                     LLIVDPDAATLCAKGQVGEVWIRGASVARGYWRADAATERTFRARLASDPDTTYLRSG
                     DLGFVHDGQLFITGRRKDLIIIRGENHYPQDLEWALQGRHPAVRPNSAVALSLDDARG
                     EQLVLVTEVYPDHAIDPAPVFAAMREAAAEYGLQLGVAVLCPPGAVPKTSSGKVQRGP
                     TKQLLRSGELRTLARWDLPTAEATDDPDEDLRDLLSNSDLAARTDLLRDYLGRLIAAR
                     LRVATVPSDVPVAQHGLDSLASVEIAERLSFQLRAPVLLGELHGTIDELAQLLAARRA
                     EHTLVPAPGDRHADFPLHGIQQAYWVGRAGGALGGVSCHAYHELECIDLDLPRLGRAW
                     QQLIARHDALRLVIDGDGRQRVLADVPPYTIARDDLGPLTPADRDTRLLATRDEISHQ
                     VLPADQWPLFRIHASALDEHRTRLHLGIDLLLADGASLSLLMHEWSRLYAGDTLPELP
                     PCSFRDCALAELAARDSEHYRASLAYWSAHRPDVPGPELPLARRPEDLTGPARFVRRR
                     AVIAPDVWSAIETEARRRHLGPSVVLCAAFTEVLATWSARPELLLNMTTFRRPPIHPR
                     VHEIVGDFTTMALLTCRPAASFAALAAALQQQFHEQLDHRVVHGVEVLREWSRSGPVD
                     VPVVFTSMIRSDGTGQWDTTWLGDEVHAISQTPHVWLDHQVLECNGELVSRWDAVEAL
                     FPPCMLDAMFAAFQARVSALAEQPALWDSPTPDLLPATERATIAAANATDAPFTATTL
                     PALFHTQAHLRPH"
     gene            complement(3475..4626)
                     /locus_tag="VGB85_16900"
     CDS             complement(3475..4626)
                     /locus_tag="VGB85_16900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135767.1"
                     /translation="MSEQASAWLLYAGTQHASGPAELVRETIDLPAIDGDRVLAEPLY
                     GCWAGNMTHALERRPADICRQRREDRVVLGNAGVVRILSVGPDVRALRPGQLALLFSA
                     SVLDKYGYPIKALAYDAAGTMGCLATRMVIREHELLLLPEPTRHSLPQWAAFSGASIT
                     AWSNWELAHGTLRLQLHHDELPAPHVWGWGGGTTLAQLDLARRHGCNTVQLSSDPHRR
                     ALIDRTGVTALAREQFPALAFDEARFTGDLTYRRAYLESETAFLREVERRTDGEGVHI
                     FVDYIGLPVHRVTLKALARQGIVTTAGWKLGMALSHLRAAECIARHQFIHTHFARRSQ
                     GIAAMAYAEAHGWIPEVDEAVAFDRIPELARAHAGGSTGFFPIFAVNPS"
     gene            complement(4623..>6247)
                     /locus_tag="VGB85_16905"
     CDS             complement(4623..>6247)
                     /locus_tag="VGB85_16905"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEY0135768.1"
                     /translation="LHDAGIPTDSLHGVAAGVYVGACNHDYDALQPAPADAHTLTGSL
                     MSVLSGRLSHVLGVRGPSLTIDTACSSSLVALHLACASLRTRETDLALAGGVNLILTP
                     DSHIRLSRMRALAPDGRCRTFDAAASGYVRGEGVGVLVLKRLGDALQSADRIWAVVRG
                     SAVNHDGRSTGLTAPNGLSQREVIARALADAKLTTDAIDHIEAHGTGTPLGDPIEVES
                     LRDLFSGGPPCVLGSVKTNIGHLEAAAGVAGVIKVALSLAHERIPRHLHYREHNPRID
                     LGTLEVARDARPWPRGPRERLAGVSSFGISGTNAHVILGEAPPPRSEPLPARSEPFVL
                     PLSAHSPTALAQLARAWAEHLRRSDVPLADLVHTAATRRTHHAHRLAVTGTSAEELAR
                     ALDAHTPSTGELATKYMHGAAIAWADVYPGRRPPAPVPRYPWQHARYWIDTPVDEPAA
                     PPTTTPRPTSRAELEQHITAATREVLGFDAGLRIPGAQDLRDLGLDSLLALDLAVAIG
                     RRLDMTLANSFAADYPTIAAMTDALARTLERP"
BASE COUNT          832 a         1836 c         2761 g          818 t
ORIGIN      
        1 cgtgcgggcg cagatgggcc tgcgtgtgga acagggctgg cagggtggtc gcggtgaacg
       61 gggcgtcggt agcgttggcg gcggcgatcg tggcgcgctc ggtggccggc agcaggtcgg
      121 gggtcgggct gtcccaaaga gcaggttgtt cggcgagggc gctgacgcgg gcctggaacg
      181 cggcgaacat ggcgtcgagc atgcacgggg ggaacagggc ctcgacggcg tcccagcgcg
      241 acacgagctc gccgttgcac tcgagcactt gatggtcgag ccagacgtgc ggggtctggc
      301 tgatcgcgtg gacctcgtcg ccgagccagg tggtgtccca ctgaccggtg ccgtcgctgc
      361 ggatcatgct ggtgaagacg acagggacgt cgacgggtcc gctgcgcgac cactcgcgca
      421 ggacctcgac gccgtgcacg acgcggtgat cgagctgctc gtggaactgc tgttgcaggg
      481 ccgcggcgag ggcggcgaag ctggcggcgg gccggcaggt cagcagcgcc atcgtggtga
      541 agtcgccgac gatctcgtgc acgcgcgggt ggatcggcgg gcggcgaaag gtcgtcatgt
      601 tgagcaggag ctcggggcgc gcggaccagg tggcgagcac ctcggtgaag gcggcgcaca
      661 ggacgacgga gggaccgaga tgacggcggc gggcctcggt ctcgatcgcg gaccagacgt
      721 cgggggcgat gacggcgcgg cggcggacga agcgggcggg gccggtcagg tcctccggac
      781 gacgggcgag cgggagctcg gggccgggca cgtcggggcg atgcgcggac cagtaggcga
      841 gcgaggcccg gtagtgctcg ctgtcgcggg cggcgagctc ggcgagggcg cagtcgcgaa
      901 agctgcacgg cgggagctcg gggagcgtgt cgccggcgta caggcgcgac cactcgtgca
      961 tcagcaagga caggctggcg ccgtcggcga gcagcaggtc gatgccgagg tgcaggcggg
     1021 tgcggtgctc gtcgagcgcg ctggcgtgga tgcgaaacag cggccactgg tccgcgggca
     1081 ggacctggtg cgagatctcg tcgcgggtgg cgagcaggcg agtgtcacga tcggcgggcg
     1141 tgagggggcc gaggtcgtcg cgggcgatcg tgtagggcgg gacgtcggcg agcacgcgct
     1201 ggcggccgtc gccgtcgatg acgaggcgca gggcgtcgtg gcgggcgatc aactgctgcc
     1261 aggcgcgccc caggcggggg aggtcgaggt cgatgcactc gagctcgtgg taggcgtggc
     1321 acgagacgcc cccgagcgcg ccgccggccc ggccgaccca gtaggcctgc tggatgccgt
     1381 gcagcgggaa gtcggcgtgg cggtcccccg gagcggggac gagggtgtgc tcggcgcgac
     1441 gagctgcgag gagctgggcg agctcgtcga tcgtgccgtg gagctcgccg agtaagacgg
     1501 gggcgcgcag ctggaagctc aggcgctcgg cgatctcgac gctggcgagc gagtcgaggc
     1561 cgtgctgtgc gaccgggacg tcggacggga cggtggcgac gcgcaggcgg gcggcgatca
     1621 ggcgcccgag gtagtcgcgc aggaggtcgg tgcgggccgc gaggtcgctg ttcgaaagca
     1681 ggtcgcgcag gtcctcgtcg gggtcgtcgg tggcctcggc ggtcgggagg tcccagcgag
     1741 ccagcgtgcg cagctcgccg gagcgcagca gctgtttggt cgggccgcgc tggaccttgc
     1801 cgctcgaggt cttgggcacg gcgccgggcg ggcacagcac ggcgacgccg agctgcaggc
     1861 cgtactcggc ggcggcctcg cgcatcgcgg cgaacacagg ggcggggtcg atcgcgtgat
     1921 cggggtagac ctcggtgacg agcacgagct gctcgccgcg ggcgtcgtcg agagacagcg
     1981 cgacggcgct gttcgggcgc acggcggggt ggcggccctg cagcgcccac tcgaggtcct
     2041 gcgggtagtg gttctcgccg cggatgatga tcaggtcctt gcggcggccg gtgatgaaca
     2101 gctggccgtc gtggacgaag ccgaggtcgc cgctgcgcag gtaggtggta tcggggtcgc
     2161 tggcgaggcg ggcccggaag gtgcgctcgg tggccgcgtc ggcgcgccag tagccgcgcg
     2221 cgaccgaggc gccgcggatc cacacctcac cgacctggcc ttttgcacag agcgtggcgg
     2281 cgtcgggatc gacgatcagg agctcggtgt cgccggtcgg cacgccgcag ccggcgacga
     2341 cgcgggtgcg ggtcgtatcg tcggcgaggg cgacgcggcc gcgctcgagc gcgtcggcgg
     2401 ccacttttaa aaatatgccg cggtgggggt ggggctcgga ggtcagctcg gcggtcgcct
     2461 cggacatgcc gtagctgtgg gagagggcgt cggggtcgag gccgtacggg gcgaaggcgg
     2521 cgaggaaggc ggcctcggcg gactggcgga tcggctcggc gccgttgagg gcgtgacgcc
     2581 aggaggtcag gtcgaggtcg gtgcattcgt gcgggcgcgt gcgcgcgacg accagctcgt
     2641 aggcgaagtt gggggcgacg ctgtgggtgc cgcggtacct cgtgatcgca cgcagccacc
     2701 gcagcgggcg cgcgaggaag gcggcgggtg gcatcgagat cgcggtgaag ccgatgtaca
     2761 gcggcagcgc gacgccgtag acgaggccga ggtcgtggta ggtcgggacc cagtagacca
     2821 tcacgctggt cgggtcgagc gcgtagacct cgttgagcgc gccgcagttg tgcagcaagt
     2881 tggcgtggtc gatgacgacg cccttggggt cggcggtcga cccggaggtg tattgcaggt
     2941 aggcgacggt gtcggcggcg agggcgggcg gggtccactc gtcggcgagc gcgaggtcga
     3001 ggtcatcggt ggtgaaccat gggatcgcgg gtaatgcggc gcgtagctcg tcgccggcgg
     3061 cgagcacggc ggggacggtg aagatcgcgg agacctcggc gtcggcgcag atcgagagca
     3121 ggcggcgcag ggccgggccg cggcggccgg cgtcgggcgg cggcacgggc acgccgacga
     3181 ggccggcgaa catgcagccg agcagggccg cggcgaactc gaggccgggc gggtgcggga
     3241 gcaggacgcg ggcgccgggc gggagatggc gcgacagcgt gacggcgatc gcacgcgcct
     3301 gggtgagcag ggcggcgagc gtgagggtct gctcgtcgtc gtcgccgtcg acgaggtggc
     3361 ggaagatgac gaggtcgggc gtcgcggcgg cgcggtggag cagcaggtcg acgagggtgc
     3421 gcacgtgcgc gggcgggtgg tgctcgcggc gctgccatgg gagggcgtgg aggatcatga
     3481 ggggttcacc gcgaagatcg gaaaaaagcc ggtgctgccg ccggcgtgag cgcgcgcgag
     3541 ctcggggatg cgatcgaagg cgacggcctc gtcgacctcc gggatccagc cgtgggcctc
     3601 ggcgtacgcc atcgcggcga taccctgcga gcggcgcgcg aagtgggtgt ggatgaactg
     3661 atggcgggcg atgcactcgg cggcgcgcag gtgcgagagc gccatgccga gcttccagcc
     3721 ggcggtggtg acgatgccct ggcgcgcgag cgccttgagg gtgacgcggt ggacgggcag
     3781 gccgatgtag tcgacaaaga tgtgcacgcc ctcgccgtcg gtgcggcgct cgacctcgcg
     3841 gaggaaggcg gtctcgctct cgaggtaggc gcggcgatag gtgaggtcgc cggtgaacct
     3901 ggcctcgtcg aaggcgagcg cggggaactg ctcgcgcgcg agggcggtga cgccggtgcg
     3961 atcgatcagg gcgcggcggt gcgggtcgga ggacagctgc acggtgttgc agccgtgacg
     4021 gcgggcgagg tcgagctgcg cgagggtggt gccgccgccc cagccccaca cgtgcggggc
     4081 gggcagctcg tcgtggtgca gctgcaggcg cagggtgccg tgggcgagct cccagttgga
     4141 ccaggcggtg atcgaggcgc cggagaaggc ggcccactgc ggcagcgagt ggcgggtcgg
     4201 ctcgggcagg aggagcagct cgtgctcgcg gatcaccatg cgcgtggcga ggcaacccat
     4261 ggtcccggcg gcgtcgtagg cgagggcctt gatcgggtag ccgtacttgt cgaggacgga
     4321 ggcgctaaac agcagcgcga gctggccggg ccgcaacgcg cggacgtcgg ggccgacgga
     4381 gaggatccgg acgacgccgg cgttgccgag cacgacgcga tcctcgcggc gctggcggca
     4441 gatgtcggcg ggccttcgct cgagcgcgtg ggtcatgttg ccggcccagc agccgtacag
     4501 gggctcggcg agcacgcggt cgccgtcgat cgcgggcagg tcgatcgtct cgcggacgag
     4561 ctcggccggc ccgctcgcgt gctgcgtgcc ggcgtacagc agccacgcgc tggcctgctc
     4621 gctcacggcc gctccagggt gcgcgcgagc gcgtcggtca tcgcggcgat cgtcgggtag
     4681 tcggcggcga aggagttggc gagggtcatg tcgaggcggc gaccgatcgc caccgcgagg
     4741 tcgagcgcga gcagggagtc gaggccgagg tcgcggaggt cctgcgcgcc ggggatccgc
     4801 aggccggcgt cgaagccgag gacctcgcgc gtcgcggcgg tgatgtgctg ctcgagctcg
     4861 gcgcgcgagg tggggcgtgg tgtggtggtc ggtggcgccg cgggctcgtc cactggcgtg
     4921 tcgatccagt agcgcgcgtg ttgccacggg tagcgcggga ccggggccgg cggccggcgg
     4981 cccggataga cgtcggccca ggcgatcgcg gcgccgtgca tgtacttcgt ggcgagctcg
     5041 ccggtcgacg gggtgtgtgc atcgagggcc ctggcgagct cctcggcgct ggtgccggtg
     5101 acggcgaggc ggtgggcgtg gtgggtgcgg cgggtcgcgg cggtgtgcac gaggtcggcg
     5161 agcgggacgt cgctgcggcg caggtgctcg gcccaggcgc gggcgagctg cgcgagcgcg
     5221 gtcgggctgt gggcggacag cgggagcacg aagggctcgc tgcgggccgg cagtggttcg
     5281 ctgcgcggag gcggggcctc gccgaggatc acgtgggcgt tggtgccgct gatgccgaag
     5341 gagctgacgc cggcgaggcg ctcgcgcggg ccgcggggcc acgggcgggc gtcgcgtgcg
     5401 acctcgaggg tgccgaggtc gatgcgcggg ttgtgctcgc gatagtggag gtgcctcggg
     5461 atgcgctcgt gggcgaggga gagggcgacc ttgatgacac cggcgacgcc ggcggcggcc
     5521 tcgaggtggc cgatgttggt cttgacggag ccgagcacgc agggcgggcc gccggagaac
     5581 aggtcgcgca ggctctcgac ctcgatcggg tcgccgagcg gggtgccggt gccgtgggcc
     5641 tcgatgtgat cgatcgcgtc ggtggtcagc ttggcgtcgg cgagggcgcg ggcgatcacc
     5701 tcgcgctggc tgaggccgtt gggcgcggtg aggccggtgc tgcgaccgtc gtggttgacg
     5761 gcggagccgc ggacgacggc ccagatccgg tcggcgctct ggagagcgtc gccgaggcgc
     5821 ttgagcacga gcacgccgac gccctcgccg cgcacgtagc cgctggcggc cgcgtcgaag
     5881 gtgcggcagc ggccgtcggg ggccagcgcg cgcatgcgag acagacggat gtggctgtcg
     5941 ggagtgagga tcaggttgac gccgccggcg agggcgaggt cggtctcgcg ggtccgcagg
     6001 ctggcgcacg cgaggtgcag ggcgaccagc gaggacgagc aggcggtgtc gatcgtcagg
     6061 ctcgggccgc gcacgccgag cacgtgcgag agccgcccgg acagcacgct catcaggctg
     6121 ccggtgaggg tgtgggcgtc ggcgggcgcg ggttgcaggg cgtcgtagtc gtggttgcag
     6181 gcgccgacgt acacgccggc ggcgacgccg tgcagggagt ccgtggggat gccggcgtca
     6241 tgtaaag
//
LOCUS       DASWNJ010000998         2795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_88194, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000998 DASWNJ010000000
VERSION     DASWNJ010000998.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88194"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VGB85_16910"
     CDS             complement(<1..816)
                     /locus_tag="VGB85_16910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135769.1"
                     /translation="MAGSTSTISATSSSSSSSMTPESSSSISGEGCVDFVASAGLAGF
                     SGVAGVGPGGLRPRAPGRGRATGTFSLEVSGASVFAGVSLVTASLAAGVSDGGGGGGL
                     KTGSGVPSPKRVGSGVPSPKVLVGSLRAAEPSVSVSPWASASRRATEPSVAPDVALVV
                     ASLVGASVFAASVFVASLVAGASLGAALVAASLDASPVAGASLDASPVAGASLDASPV
                     AGASLFGASLIAGASLDASPVAGASLFGASLIAGASLGDASLVAVSLVTGFSSA"
     gene            794..2527
                     /locus_tag="VGB85_16915"
     CDS             794..2527
                     /locus_tag="VGB85_16915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135770.1"
                     /translation="MVEVEPAKPTPPPKPAPKAPPGRPRPPAGRPPPPKRGPPPRSED
                     SKVTSAPDDSKAQPVPEDSKAGLPPEDSKATPPTEDSKAATPTESPVSEDSKTATPTE
                     TPVSEDSKAATPAEAPVSEDSKAATPAEAPVSEDSKTTPPAEAPATDSESAATGDAPA
                     TPATAAPVTDGESATPADAADKPTTPAADAPVADSKPTTDDAPVADSATPSGTADKST
                     TDDASTTDSATLAGEPAAAAPVPEAAESAPSDATPAAEAPVPRDSKPARSAGEPPRPP
                     VPRDSKAADAHIGPEAAEIAADVTPAGEDAETDELVPAADARPGEPNPGDTAMLAIDA
                     VLAPEGEQTDITDEIQELRFFISGRFEDDAQFAYLDLQRRFPGHPALAEFADRFATGA
                     KLESAAAPVMLDDPRPASVPSRGAGAAKVAVLDDDDEDTFLASIFDEPVTRAPGKSAK
                     PRRAVASLDDAADPQTFFDLGTAYREMGLEDDALTQFDLAAKDPRWTARARIMSASLR
                     IQRGEHAQAIDDLNLAIDFATDVDQRSEAGYELGVLYQTLGDTANAIEALSAVAEGYR
                     DRDDRLKTLGG"
BASE COUNT          463 a         1142 c          958 g          232 t
ORIGIN      
        1 cgcggacgag aagcccgtca cgagcgacac cgcgaccagc gatgcatcgc cgagtgacgc
       61 gcccgcgatc agcgatgcac cgaagagtga cgcgcccgcg actggcgagg catcgagtga
      121 cgcgcccgcg atcagcgatg caccgaagag cgacgcgccc gcgactggcg aggcatcgag
      181 cgacgcgccc gcgactggcg aggcatcgag cgatgcgccc gcgactggcg aggcatcgag
      241 cgatgccgcg acgagagctg cgccgagcga cgcacccgcg acgagtgacg ccacgaagac
      301 cgaggccgcg aagaccgatg ccccgacgag tgacgccacg acgagcgcca cgtccggcgc
      361 gaccgagggt tcggtcgcgc gtcggctcgc cgaggcccag ggtgacacgc tgaccgaggg
      421 ctcggccgct cgcaggctgc cgaccaggac cttcggcgag ggcactccgc tgccgactcg
      481 cttcggcgag ggcactccgc tgccggtctt caagccgcca ccgccgcccc cgtccgagac
      541 cccggccgcg agcgaggcgg tcacgagcga caccccggcg aagacggacg cccccgagac
      601 ctccagcgag aacgtcccgg tcgcccgccc gcgccccggc gctcgcggac gcaagccgcc
      661 cggcccgacc ccggcgacgc ccgagaagcc ggcgagcccc gccgacgcga cgaagtcgac
      721 gcagccttcc ccggagatcg aagagctcga ctccggcgtc atcgaggagc tcgacgacga
      781 cgaggtcgcc gagatggtcg aggtcgagcc cgccaagccc accccgccgc cgaagcccgc
      841 acccaaggcg cctcccggac gcccccgtcc gcccgccggt cgcccgcccc cgcccaagcg
      901 cggacccccg ccccgcagcg aggacagcaa ggtcacgtcc gcgccggacg acagcaaggc
      961 gcagcctgtc cccgaggaca gcaaggccgg cctgccgccc gaggacagca aggcgacgcc
     1021 gccgaccgag gacagcaagg ccgcgacgcc gaccgagtca cctgtctcgg aggacagcaa
     1081 gaccgcgacg ccgaccgaga cacctgtctc ggaggacagc aaggccgcga cgccggccga
     1141 agcacctgtc tcggaggaca gcaaggccgc gacgccggcc gaggcacctg tctcggagga
     1201 cagcaagacc acgccgccgg ccgaggcgcc cgcgacagac agcgagtccg ccgccaccgg
     1261 ggacgcgccc gcgacccccg ccaccgccgc gcccgtgacg gacggcgagt ccgcgacgcc
     1321 ggccgatgcc gccgacaaac cgacgacgcc ggccgccgac gcgcccgtcg cggacagcaa
     1381 gccgacgacg gacgacgcgc ccgtcgcgga cagcgcgacg ccgtccggga ccgccgacaa
     1441 gtcgacgacg gacgacgcgt ccaccacgga cagcgcgacg ctggccggcg agcccgcagc
     1501 cgcggcgcct gtccccgagg ccgccgagag cgctccttcc gacgcgacgc cggccgccga
     1561 ggcacctgtc cccagggaca gcaagcccgc gaggtccgcc ggcgagcccc ccaggccgcc
     1621 tgtcccccgg gacagcaagg ccgcggacgc ccacatcggc cccgaggcgg ccgagatcgc
     1681 ggccgacgtt acccccgcgg gcgaggacgc cgagaccgac gagctggtgc ccgcagcgga
     1741 cgccaggccg ggcgagccga accccggcga cactgcgatg ctggcgatcg acgcggtgct
     1801 cgctcccgag ggcgagcaga ccgacatcac cgacgagatc caggagctgc gcttcttcat
     1861 ctccggccgc ttcgaggacg acgcccagtt cgcctacctc gaccttcagc gccgcttccc
     1921 gggtcacccg gcgctggccg agttcgccga ccgcttcgcg accggcgcca agctcgagtc
     1981 cgccgccgcg ccggtgatgc tcgacgaccc gcgcccggcc tcggtgccgt cgcgcggcgc
     2041 gggtgctgcc aaggtggcgg tgctcgacga cgacgacgag gacaccttcc tcgcctcgat
     2101 cttcgacgag ccggtcacgc gggcgcctgg caagtccgcg aagccgcggc gcgccgtcgc
     2161 ctcgctcgac gacgccgccg atccgcagac cttcttcgac ctcgggaccg cctaccgcga
     2221 gatgggcctc gaggacgacg cgctgaccca gttcgacctc gccgccaagg atccgcgctg
     2281 gaccgcgcgg gcgcgcatca tgagcgccag tctccggatt cagcgcggtg agcacgccca
     2341 ggcgatcgac gacctcaacc tggcgatcga cttcgccacg gacgtcgacc agcgcagcga
     2401 ggccggctac gagctcggcg tgctctacca gaccctcggc gacaccgcca acgcgatcga
     2461 agcgctcagc gccgtcgccg agggctaccg cgaccgcgac gaccgcctca agaccctcgg
     2521 cggctgagcg gccgccgggc gagcggcacc tgtcccaagg ggcaggtgcg cgacgctcac
     2581 ggcccgggcg atcgtcaggc gcctgtccgg cgggacaggt ctcgccaccg cctcacggac
     2641 cggcgagccc gcgcagcacg cccaggcgca cgcccagctc ctccgccgcg tgaccctgcg
     2701 ccggcgcgag gccgtcgagc agcgcccgcg cctggccggc ccgcgcccgc gcgagcaacc
     2761 accggcacac cgcgagcagc tgcggtcgca gcacc
//
LOCUS       DASWNJ010000999         2931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_195647, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010000999 DASWNJ010000000
VERSION     DASWNJ010000999.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195647"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VGB85_16920"
     CDS             complement(<1..884)
                     /locus_tag="VGB85_16920"
                     /inference="COORDINATES: protein motif:HMM:NF040941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-like YCDxxxxGGGW domain-containing
                     protein"
                     /protein_id="HEY0135771.1"
                     /translation="MLMTLSLPTAGCLSPQSGETTTDVMLGSDTGMTVAGSTSGDSST
                     QPAIMTTTDEPETTDAPDTASTGTTSTTDPPDTTMDGGSCGNGSIEAGEECDEGEANA
                     NEGTCTKSCKSATCGDGYLQLGAGEVCDDGDNDGSYGGCASDCKSLGPHCGDGVVTDM
                     VEACDGGDPWNVASGCLPNCEYAQSCKQIRESFPDDDTVIDDVYLIKRANNAPFPVYC
                     DMDADGGGYTFLKVALPEGQTMTAGFAEQQCAEFGMKLLALRTPMHVVAAGLAAKSAM
                     LEPIGGGTTKGTVEYLSIL"
     gene            complement(1084..2304)
                     /locus_tag="VGB85_16925"
     CDS             complement(1084..2304)
                     /locus_tag="VGB85_16925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0135772.1"
                     /translation="MSSHIHDVIIVGCGPAGSTAANLLAQAGRDVLVLERESFPRFHI
                     GESLLPIDLPLFDRLGLEIDARDFKFKQGAEFLDERTGEFAFFSFADALPGTPSHAWQ
                     VERAVFDDTLARQAIARGAQVRFGEAVTGIDIETDEVRVRTASGEHRGRFLFDATGQD
                     AWLARRARSVAPIREIGRAAVFTHFEDLSPAAVAEIGSQGNIKVLMVEDGWHWVIPLI
                     GGRLSVGLVKWRGKLDEAALEAALAASPVIQRLTAGARRGPTRTIANWSYKNLRPHGP
                     RWSCIGDAAAFLDPVFSSGVSLAMLGAERAVDLLLPALASSTEADPGLMGPWSTYMQP
                     AYDTFEQLIRRFYHTKIVAHLFFAAEPDPALRRGLISVLAGDVWRRDNPFQTMLETSR
                     LQPKPPVASAEPEA"
     gene            complement(2301..>2931)
                     /gene="fabG"
                     /locus_tag="VGB85_16930"
     CDS             complement(2301..>2931)
                     /gene="fabG"
                     /locus_tag="VGB85_16930"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEY0135773.1"
                     /translation="NYRSDVGAAEAVAARIREAGGSVTLIPFDVGDPEASAAAMAEIL
                     ADPAPLGVIVNNAGVARDNPLPALEREDWDLVMRTTLGGFYNVTRPAIMPMVRRRWGR
                     IINMSSVSAVIGNRGQVAYAAAKAGIIGATKSLSLELARRGITVNAVAPGLIETDMIK
                     DVPLEHVMPRIPAQRLGQPAEVAALVAFLASDAAGYITGQVIGINGGMA"
BASE COUNT          415 a         1021 c         1028 g          467 t
ORIGIN      
        1 aggatcgaga ggtactccac ggtgcccttg gtcgttccac cgccgatggg ctcgagcatg
       61 gcggacttgg cggcgagccc cgccgccacg acgtgcatcg gcgtccgcag ggccagcagc
      121 ttcatgccga actcggcgca ctgttgctcg gcgaacccgg cggtcatcgt ctgaccctcg
      181 ggcagcgcga ccttgaggaa ggtgtagccg ccgccgtcgg catccatgtc gcagtagacc
      241 gggaacggcg cgttgttggc acgcttgatc agatacacgt cgtcgatcac cgtgtcgtcg
      301 tccgggaagc tctcgcggat ctgcttgcag ctctgcgcgt actcgcagtt gggcaggcag
      361 ccgctggcga cgttccacgg gtccccgccg tcgcaggcct cgaccatgtc ggtgaccacc
      421 ccgtcgccgc agtgcgggcc gaggctcttg cagtcgctcg cgcagccacc gtagctgccg
      481 tcgttgtccc catcgtcgca gacctcgccc gcgccgagct gcaggtagcc gtcgccgcac
      541 gtcgccgact tgcatgactt ggtgcaggtg ccctcgttcg cgttggcttc cccctcgtcg
      601 cactcctcgc cggcctcgat cgacccgttg ccgcagctcc caccgtccat ggtcgtgtcc
      661 ggtgggtcgg tggtgctcgt cgtgcccgtc gacgccgtgt ccggagcatc ggtcgtctcc
      721 ggctcgtcgg tggtcgtcat gatcgcgggc tgcgtcgagc tgtccccact ggtgctgccg
      781 gcgacggtca tgccggtatc gctgccgagc atgacgtccg tcgtcgtctc gccgctttgg
      841 ggtgacaggc acccggcggt cggcaacgac agcgtcatca acatcgtcag cgcgcaccca
      901 ggggtcgcgg agcgacgcgc atcgatcgaa cgggggacct cgttggcagg ttgcggcttc
      961 gacatgcgac cgactcaggg aacccgcttt ggcgcgaaat tgcaaccagg ggaattccat
     1021 ctcccgccgc gtcctgacgc gacattctgc aaattacgta agccgcctta catatttggc
     1081 agttcaagct tcgggttcgg cgctggcgac cggcggcttc ggttgcaggc gcgacgtctc
     1141 cagcatggtc tggaacgggt tgtcgcggcg ccacacgtcc ccggccagca cgctgatcag
     1201 gcccctgcgc agcgccggat cgggctcggc ggcgaagaac aggtgggcga cgatcttggt
     1261 gtgatagaag cggcggatca gctgctcgaa ggtgtcgtag gccggctgca tgtaggtcga
     1321 ccacggcccc atcaacccgg gatcggcctc tgtgctcgag gccaacgcgg gcagcagcag
     1381 atccaccgcc cgctccgcgc cgagcatcgc cagcgacacg cccgacgaga acaccgggtc
     1441 gaggaaggcg gcggcgtcgc cgatgcatga ccagcgcggg ccgtgcgggc gcaggttctt
     1501 gtacgaccag ttggcgatcg tgcgggtcgg cccacggcgc gcgcccgcgg tcagccgctg
     1561 gatcaccggc gaggcggcca gcgcggcctc gagggcggcc tcgtcgagct tgccgcgcca
     1621 cttgaccagg ccgacgctca ggcgcccgcc gatcagcggg atcacccagt gccagccgtc
     1681 ctcgaccatc agcaccttga tgttgccctg gctgccgatc tccgcgaccg cggcgggcga
     1741 gagatcctcg aagtgggtga acaccgccgc gcgaccgatc tcgcggatcg gcgcgacgct
     1801 gcgggcccgg cgggccagcc aggcgtcctg cccggtggcg tcgaacagga agcgcccgcg
     1861 gtgctcgccg ctcgcggtgc gcacgcggac ctcgtcggtc tcgatgtcga tccccgtgac
     1921 ggcctcgccg aagcggacct gcgcgccgcg ggcgatcgcc tggcgcgcga gcgtgtcgtc
     1981 gaacaccgcg cgctcgacct gccacgcgtg cgagggtgtg cccggcaacg cgtcggcgaa
     2041 gctgaagaac gcgaactcgc cggtgcgctc gtcgaggaac tcggcgccct gcttgaactt
     2101 gaagtcccgc gcgtcgatct cgaggccgag gcggtcgaac agcgggaggt cgatcggcag
     2161 cagcgactcg ccgatgtgaa agcgcgggaa actctcgcgc tcgagcacga ggacatcgcg
     2221 tccggcctgc gcgagcaggt tcgccgcggt cgagccggcc gggccgcagc cgacgatgat
     2281 cacgtcgtgg atatgcgagc tcatgccatg cctccgttga tcccgatcac ctggccggtg
     2341 atgtagccgg cggcgtcgct ggcgaggaag gcgaccagcg cggcgacctc ggccggttgc
     2401 ccgagcctct gcgcggggat ccgcggcatc acgtgctcga gcgggacatc cttgatcatg
     2461 tcggtctcga tcaggccggg ggccaccgcg ttgacggtga tcccgcggcg cgcgagctcg
     2521 agcgacagcg acttcgtcgc gccgatgatc cccgccttgg ccgccgcgta ggcgacctgg
     2581 ccgcggttgc cgatcaccgc cgagaccgag ctcatgttga tgatgcgacc ccagcggcgg
     2641 cgcaccatcg gcatgatcgc cgggcgcgtc acattgtaaa aaccgccgag ggtggtgcgc
     2701 atgacgaggt cccagtcctc gcgctcgagc gccggcagcg ggttgtcgcg cgcgaccccg
     2761 gcgttgttga cgatcacccc cagcggcgcc gggtcggcga ggatctcggc catcgccgcg
     2821 gcgctggcct cggggtcgcc gacgtcgaag gggatgagcg tgacgctgcc gcccgcctcg
     2881 cggatgcggg cggcgacggc ctcggccgcg ccgacgtcgc tgcgatagtt g
//
LOCUS       DASWNJ010001000         3528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_654495, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001000 DASWNJ010000000
VERSION     DASWNJ010001000.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654495"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3528)
                     /locus_tag="VGB85_16935"
     CDS             complement(<1..>3528)
                     /locus_tag="VGB85_16935"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEY0135774.1"
                     /translation="PTPSRSAPRDLIELIAREAAAAFDLPEVDPLALFGDLGVDSVMA
                     LRLRSRLTRALGRELPLTLAYDYPSIVALARHLGPTDTSPTISSTPHDSAEPIAVIAM
                     ACRLPGSVETPEDLWAVLDKGRDATTDVPPSRWDAAAIFSADPHTPGTSYCTRGGFLG
                     DLQHFDAEFFGLAPREARALDPQQRLLLEVTWETLERAGIVPAALTGHDVGVYIGLSA
                     HEYAWMEGHSPAEVLDGYGITGTTASTASGRLAYVLGLEGPALTVDTACSSSLVTVHL
                     ACQALRAGECSMALAGGVTLLQTPQLFIEFSRLQGLAPDGRSKAFSADADGVGWSEGC
                     GMVLLKRLSEARRDGDPILAVIRGSAINQDGHSNGLTAPNGPAQERVIRRALELAQVS
                     PADVDQVEAHGTGTRLGDPIEARALAAVYGRDRRDGAPLWLGSSKSNMGHTLSAAGVA
                     GLMKIALSLIHQQMPPTLHAATASPHIDWHTSGLALLQSSQPWPARPDRPRLGGVSSF
                     GVSGTNAHLILAEAPPRPTAHRQSPPVDHALLVSGKTDAALRSQAARWADWLTAHPDT
                     TWSDVIHTAARRRTHFRRRAALHVAGPTAAIAALRALADGRPHPDLLTATASGRLAVL
                     FTGQGSQRAGMGTQLAASHPVFRDALARCCAQLDLHLEHPLAAQLHDADALAQTHLTQ
                     PALFALEVALYRQWEAWGLEPDFLVGHSVGELAAAHVAGVLSLADAARLVCARGRLMQ
                     AMPTGGAMASISASEAELLPLLGPGVAIAGLAGAQQSVVSGDTTAVDALVAHFAALGR
                     RTRRLRVSHAFHSPHMDGMLAQLAEVVATCDLRPPRIPIVSTLTGRRVDDHTLADPNY
                     WSAQARQAVRFLDAVQTTWHAGARVFLEVGPDATLSAMVPACLPDADAVTVITSLHRD
                     RDEPASLARARSAIHVAGVHLEPRALGPDDGELVALPTYAWQRALCWRDPPAATAHGT
                     PARFELGGAWRALPDGGFLHELHVGTRAQPYLADHRVHGQVVAPGTFHVSVLLALAAE
                     RWPGEPVELTRVVFRGPLLLPAHDLLLSAHLRPDGDGFGVTLATHDTDWQVHVEAHIA
                     RAQQLPRPLTTPHTPPDLELGFTDIVTARAAVDVVWGPRWAWTAAIHRHGDRLVTQLE
                     RPADAPGLAHAPIDPVLLDNASAAGMAHR"
BASE COUNT          454 a         1060 c         1580 g          434 t
ORIGIN      
        1 cagcggtgcg ccatgccggc ggcggaggcg ttgtcgagca ggacggggtc gatcggcgca
       61 tgcgcgaggc cgggggcgtc ggcggggcgc tcgagctgcg tgacgagccg gtcgccgtgg
      121 cgatggatcg cggcggtcca ggcccagcgt ggaccccaga cgacgtcgac ggcggcgcgg
      181 gcggtgacga tgtcggtgaa gccgagctcg agatcggggg gcgtgtgcgg ggtggtcagc
      241 gggcgcggga gctgctgcgc gcgggcgatg tgggcctcga cgtggacctg ccagtcggtg
      301 tcgtgggtcg cgagcgtgac gccgaagccg tcgccgtcgg ggcgcaggtg ggccgagagc
      361 aggaggtcgt gcgcgggcag cagcagcggg ccgcggaaca cgacgcgggt cagctcgacg
      421 ggctcgccgg gccagcgctc ggcggcgagg gcgagcagca cggagacgtg gaaggtgccg
      481 ggcgcgacga cctggccgtg cacgcggtga tcggcgaggt agggttgcgc gcgggtgccg
      541 acatgcagct cgtgcaggaa gccgccgtcg ggcagggcac gccaggcgcc gccgagctcg
      601 aagcgagcgg gcgtgccgtg ggcggtcgcg gccggcgggt cgcgccagca cagcgcgcgc
      661 tgccaggcgt aggtcggcag cgcgacgagc tcgccgtcgt cgggaccgag ggcgcggggc
      721 tcgaggtgga cgccggcgac gtggatcgcg gagcgggcgc gggcgaggga ggcgggctcg
      781 tcgcggtcgc ggtgcaggct cgtgatgacg gtgaccgcgt cggcgtcggg gaggcaggcg
      841 gggaccatgg cgctgagggt ggcgtcgggg ccgacctcga ggaacacgcg ggcgcccgcg
      901 tgccaggtgg tctgcacggc gtcgaggaag cgcacggctt gacgggcctg cgcgctccag
      961 tagttcgggt cggcgagggt gtggtcgtcg acgcggcggc cggtcagggt gctgacgatc
     1021 gggatccgcg ggggacgcag gtcgcaggtc gcgacgacct cggcgagctg cgcgagcatg
     1081 ccgtccatgt gcgggctgtg gaaggcatgg ctgacgcgga ggcgacgcgt gcggcggccg
     1141 agcgcggcga agtgagcgac gagcgcgtcg acggcggtgg tgtcaccgct gacgacgctc
     1201 tgctgcgcac cggcgaggcc ggcgatcgcg acgccgggtc cgagcagcgg gagcagctcg
     1261 gcctcgctgg cggagatcga ggccatcgcg cctccggtcg gcatcgcctg catgaggcgg
     1321 ccgcgggcgc acacgaggcg cgcggcgtcg gcgagggaga gcacaccggc gacgtgggcg
     1381 gccgcgagct cgccgacgct gtggccgacg aggaagtcgg gctcgagacc ccacgcctcc
     1441 cactggcggt acagcgcgac ctcgagggcg aacagcgcgg gctgggtgag gtgggtctgg
     1501 gcgagggcgt cggcgtcgtg cagctgcgca gcgagggggt gctcgaggtg gaggtcgagc
     1561 tgcgcgcagc agcgggcgag ggcgtcgcgg aacaccgggt ggctggcggc gagctgcgtg
     1621 cccatgccgg cgcgctggct gccctggccg gtgaacagca ccgcgaggcg acccgacgcg
     1681 gtggcggtga ggaggtcggg gtgcgggcgg ccgtcggcga gggcgcgcag ggctgcgatc
     1741 gcggcggtgg gtcctgccac gtgcagcgcg gcgcgccggc gaaaatgcgt gcggcggcgg
     1801 gctgccgtgt ggatgacgtc ggaccaggtg gtgtcggggt gggcggtcag ccagtcggcc
     1861 cagcgggcgg cctgcgagcg cagggcggcg tcggtcttgc cggagacgag gagggcgtgg
     1921 tcgacgggtg gagactggcg gtgcgcggtg gggcgcggcg gggcctcggc gaggatcagg
     1981 tgggcgttgg tgccgctgac gccgaaggag ctgacgccgc cgaggcgcgg gcgatcgggg
     2041 cgcgcgggcc agggctggga ggactgcaac aacgcgaggc cgctggtgtg ccagtcgatg
     2101 tgcgggctcg cggtcgcggc gtgcagggtc ggcggcattt gctgatggat cagcgacagg
     2161 gcgatcttca tcaggccggc gacgccggcg gccgagaggg tgtggcccat gttcgacttc
     2221 gaggagccga gccacagcgg ggcgccatcg cggcggtcac ggccgtagac ggccgcgagg
     2281 gcgcgcgcct cgatcgggtc gccgaggcgc gtgccggtgc cgtgggcctc gacctgatcg
     2341 acgtcggcgg ggctgacctg cgcgagctcg agggcgcgcc ggatcactct ttcctgcgcg
     2401 gggccgttgg gggcggtcag gccgttgctg tggccgtcct ggttgatcgc gctgccgcgg
     2461 atcacggcga ggatcgggtc gccgtcgcgg cgcgcctccg acaaacgctt gagcaggacc
     2521 atgccgcagc cctcgctcca gccgacaccg tcggcgtcgg ccgagaaggc cttgctgcgg
     2581 ccgtcgggcg cgaggccctg caggcgcgag aactcgatga acagctgcgg ggtctgtaac
     2641 agggtgacgc cgccggcgag cgccatgctg cactcaccgg cgcgtaatgc ctggcaggcg
     2701 aggtgcacgg tgacgagcga ggaggagcag gcggtgtcga cggtgagcgc cgggccctcg
     2761 aggccgagca cgtaggcgag gcggccggag gcggtgctcg cggtggtgcc ggtgatgccg
     2821 tagccgtcga ggacctctgc cgggctgtgg ccctccatcc acgcgtactc gtgcgcggac
     2881 aggccgatgt agacgccgac gtcgtggccg gtcagggcgg cgggcacgat gccggcgcgc
     2941 tcgagggtct cccaggtgac ttctaataag agccgctgct gcgggtcgag ggcccgggcc
     3001 tcgcgggggg cgaggccgaa gaactcggcg tcgaagtgtt gcaggtcgcc gaggaagccg
     3061 ccgcgggtgc aatagctggt gccgggcgtg tgcgggtcgg cgctgaagat cgcggcggcg
     3121 tcccagcgcg agggcgggac gtcggtggtg gcgtcgcggc ccttatcgag cacggcccac
     3181 aggtcctcgg gggtctcgac gctgccgggc agacggcagg ccatggcgat cacggcgatc
     3241 ggctcggcgc tgtcgtgagg ggtcgatgag attgttgggg aagtgtcggt ggggccgagg
     3301 tggcgggcga gcgccactat cgaggggtag tcgtaggcga gggtcagcgg gagctcgcga
     3361 ccgagagcgc gggtcaggcg gctgcgcagg cgcagcgcca tcacggagtc gacgccgagg
     3421 tcgccgaaca gcgcgagcgg gtcgacctcg ggcaagtcga aggcggcggc ggcttcacgg
     3481 gcgatcagct cgatcaggtc gcgcggggcg ctgcgcgacg gcgtgggc
//
LOCUS       DASWNJ010001001         1906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_53978, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001001 DASWNJ010000000
VERSION     DASWNJ010001001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53978"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VGB85_16940"
     CDS             complement(<1..549)
                     /locus_tag="VGB85_16940"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEY0135775.1"
                     /translation="MRVAIAGAGNVGRSIALELLGNGHEVLLVEREPRAMKVDTVPGA
                     EWLLADACELATLVQAGIQTCNVVVAATGDDKVNLVVSLLAKTEFGVDRVVARVNHPK
                     NEWLFNESWGVDVSVSSPRLLAAVVEEAVSVGDLVRLLTFKKGGANLVELTLADDAPL
                     VGKRIGTVPWPEDAALVAVLREG"
     gene            complement(549..1217)
                     /locus_tag="VGB85_16945"
     CDS             complement(549..1217)
                     /locus_tag="VGB85_16945"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEY0135776.1"
                     /translation="MHVVIMGCGRVGSALAKTLEGLGHSVAIIDRDPGAFRRLSADFA
                     GTKVTGVGFDRDTLGEAGIEHAHAFAAVSSGDNSNIIAARVARETFGLENVVARIYDH
                     RRAAVYQKLGIPTVATVKWTSDQVVRRLLPEGLITEWRDPSGRVALAEVAYAPSWVGE
                     RVTRMETEAGMRVAFLTRLGEGMVPHADSVVQEGDLVHVTVESSRMEEVERVLESGPT
                     AGEH"
     gene            1322..>1906
                     /locus_tag="VGB85_16950"
     CDS             1322..>1906
                     /locus_tag="VGB85_16950"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY0135777.1"
                     /translation="MSGANDVVKRLVLGRALRSDRQSEQELPKYLALPIFASDPLSSV
                     TYATQELLVVLTLGGLAYLYLTPYLAACVVLLLTVVVLSYRQLVKAYPTGGGDYEVAS
                     KNLGKRAGGVVASALLVDYVLTVAVSVSAGVDNIISAFEGLADHRVLLALMFVAFLTA
                     MNLRGVKESGKAFAVPTLSDLGQLPKRLVLGRPVR"
BASE COUNT          260 a          717 c          656 g          273 t
ORIGIN      
        1 tccctcgcgc aggaccgcca ccagcgcggc gtcctcgggc cacggaacgg tcccgatccg
       61 cttgcccacc agcggggcgt cgtcggcgag ggtcagctcg acgaggttgg cgccgccctt
      121 cttgaaggta agcaggcgca cgaggtcgcc gaccgacacg gcctcctcca cgaccgcggc
      181 gagcaggcgc ggcgaggaca ccgacacgtc cacgccccag gactcgttga agagccactc
      241 gttcttgggg tggttcaccc gcgcgacgac gcggtcgacg ccgaactcgg tcttggcgag
      301 cagcgacacc acgaggttga ccttgtcgtc accggtcgcg gcgacgacga cgttgcaggt
      361 ctggatgccg gcctgcacga gcgtcgcgag ctcgcaggcg tcggcgagca gccactcggc
      421 gcccgggacc gtgtcgacct tcatggcccg cggctcccgc tcgacgagca ggacctcgtg
      481 cccgttgccg agcagctcca gcgcgatcga gcggccgacg ttgccggcgc cggcgatggc
      541 gacgcgcatc agtgctcacc cgccgtcggg ccggactcga gcacgcgctc gacctcctcc
      601 atgcgcgacg actccacggt cacgtgcacc aggtccccct cctgcacgac gctgtccgcg
      661 tgcggcacca tgccctcgcc cagccgggtg aggaaggcga cgcgcatgcc ggcctcggtc
      721 tccatgcgcg tgacgcgctc gcccacccac gacggggcgt acgccacctc ggcgagcgcg
      781 acgcggccgc tcgggtcgcg ccactcggtg atcagcccct cgggcagcag ccggcgcacc
      841 acctggtcgg aggtccactt cacggtcgcc accgtcggga tgccgagctt ctggtagacc
      901 gccgcgcgcc ggtggtcgta gatgcgcgcg acgacgttct cgagcccgaa ggtctcgcgg
      961 gcgacgcggg ccgcgatgat gttggagttg tcgccgctgc tgacggcggc gaacgcgtgg
     1021 gcgtgctcga tgccggcctc gccgagcgtg tcgcggtcga agccgacgcc ggtgaccttc
     1081 gtgccggcga agtcggccga caggcgccgg aacgccccgg ggtcgcggtc gatgatcgcg
     1141 accgagtggc cgagtccctc gagcgtcttg gcgagggcgg acccgacccg gccgcagccc
     1201 atgatgacga cgtgcacggc gtgcccttcg aacgaccgga ccgccggcgc tggcgaaccg
     1261 aggaggacgc tacacgcgcg gcgggctccc accgcaggtc acggccgtac cctctccgga
     1321 cgtgtccggt gccaacgacg tcgtcaagcg cctggtgctc ggccgggcgc tgcgcagcga
     1381 ccggcagagc gagcaggagc tccccaagta cctcgccctc cccatcttcg cgagcgaccc
     1441 gctgtcgagc gtcacgtacg cgacgcagga gctgctcgtc gtcctgacgc tcggcgggct
     1501 ggcctacctc tacctcacgc cctacctcgc ggcctgcgtc gtgctgctgc tgaccgtggt
     1561 ggtcctgtcc taccgccagc tggtcaaggc ctacccgacc ggcggcggcg actacgaggt
     1621 ggcgtcgaag aacctcggca agcgcgccgg cggcgtcgtg gcgagcgcgc tgctcgtcga
     1681 ctacgtgctc accgtcgcgg tgtccgtctc ggccggcgtc gacaacatca tctcggcctt
     1741 cgagggcctc gcggaccacc gcgtcctgct cgcgctgatg ttcgtcgcgt tcctcaccgc
     1801 gatgaacctg cgcggggtga aggagtcggg caaggcgttc gccgtgccga ccctgtccga
     1861 cctcggacaa ctcccgaagc gtctcgtcct cggccgcccc gtccgc
//
LOCUS       DASWNJ010001002         2892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_400816, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001002 DASWNJ010000000
VERSION     DASWNJ010001002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400816"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VGB85_16955"
     CDS             complement(<1..210)
                     /locus_tag="VGB85_16955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135778.1"
                     /translation="MRPRLAPLTALLLPAALLLALGTGPGEVGVGEALHTVAAHLGLG
                     PPTPGLRDAIVWELRLPRALLAALVG"
     gene            complement(219..1079)
                     /locus_tag="VGB85_16960"
     CDS             complement(219..1079)
                     /locus_tag="VGB85_16960"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0135779.1"
                     /translation="MRRLLVPLLALVACAPPVSTTSGPARRVLSQTVLSDEILWTLGP
                     DARARVVGLSVMADDPRYSRVVDLWPPEIPRLAVTSEGLLARDPDLVVIASFTAPEVK
                     ALLTAQRVRLLEFDSFTGFADYRAHLRALATAVEAAPAGERAVAELDARLAALAATRP
                     AVALTAVAWGDGYAATGGTTFADVAAAAGLINLPEQAGLSGHVPVAIEQLVAWDPQII
                     VVSCPATAPDDPACAAAEASFAAGPGISATQAAQGRGVVAIPARDLGSTGEGMLVTAE
                     LLQARVLARP"
     gene            complement(1083..2297)
                     /locus_tag="VGB85_16965"
     CDS             complement(1083..2297)
                     /locus_tag="VGB85_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135780.1"
                     /translation="MNHPRALAPVLSASLVLACTAPDVDDTTGSADSTGEPALPGPPV
                     LDEPCTAELAAPTRLAITTTDNVTGALSILDLRTGAVTPDLALATSDSVPFHDGARLY
                     LLHRYGFDALDVLTPDLGLLAQLGLGVPDVVSSNPHALAFAGDRAYVTMFGAPAVQIV
                     DLTDPAAPTHAGSIDLTALADPDGNPEANLAIRCGDTLFITVQRLDENLGFAATGPHD
                     HLAPIDLPSGRLHDLDPDAPGVQATPLLGPWAKQWRPDPADPAGHTLLVLSSGLERLD
                     LTTLRSTWAVDPARLAAMGLVATDLTQAFDLDRTGAHAYIASYRADLSEVVLLRASLD
                     DDAPIEEIAGGMQSIGPTLEVVDDTLWFGDRSHAAAGMRAWDLRTTPPTPRFDGLPIS
                     TGLAPYAAVPIP"
     gene            complement(2294..>2892)
                     /locus_tag="VGB85_16970"
     CDS             complement(2294..>2892)
                     /locus_tag="VGB85_16970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135781.1"
                     /translation="FVAIQRLVDFAPVDLSWLVAVDLATGAAIDLDPATDGTQALPLQ
                     GPWAKQVRLDPADPSGHTVLVLTSGVERVDLPLGTSRWAVDPTLLAAAGIDGYDPQAF
                     TVAADGASVHLLATAGDYPDAAVFHVGLDGLAPATPQVLVDGLTSREKTLERVGATLW
                     VGDAAPDQPRLRRFNLDDDSELAAVSTPGDPYLVLAIP"
BASE COUNT          361 a          876 c         1320 g          335 t
ORIGIN      
        1 gccgacgagc gcggcgagca gcgcccgcgg caggcgcagc tcccagacga tcgcgtcgcg
       61 caggccaggc gtgggcgggc cgaggccgag gtgggcggcg accgtgtgca gggcctcgcc
      121 gacgccgacc tcgcccggac ctgtcccgag ggccaggagc agcgcggcgg gcagcagcag
      181 cgcggtgagc ggggcgaggc gcgggcgcat gggcggtctc aggggcgggc gagcacgcgg
      241 gcctgcagca gctccgcggt gacgagcata ccctcgccgg tgctgccgag gtcgcgggcg
      301 gggatcgcca cgacgccgcg cccctgcgcg gcctgggtcg cggagatccc gggacccgcc
      361 gcgaagctgg cctcggcggc ggcgcaggcg gggtcgtcgg gggcggtcgc ggggcacgag
      421 acgacgatga tctgcgggtc ccaggcgacg agctgctcga tggccaccgg gacatgtccc
      481 gagagacctg cctgttcggg caggttgatc aggccggcgg cggcggcgac gtcggcgaag
      541 gtggtgccgc cggtggccgc gtagccgtcg ccccaggcga ccgcagtgag ggcgacggcg
      601 gggcgggtgg cggcgagggc ggcgaggcgg gcgtcgagct cggcgacggc gcgctcgccc
      661 gcgggcgcgg cctcgacggc ggtcgcgagg gcgcgcaggt gcgcgcggta gtcggcgaag
      721 ccggtgaagc tgtcgaactc gagcaggcgg acgcgctggg cggtcagcag ggccttgacc
      781 tccggggcgg tgaagctggc gatgacgacg aggtcggggt cgcgggcgag caggccctcg
      841 ctggtgacgg cgaggcgcgg gatctcgggc ggccagaggt cgacgacccg gctgtagcgc
      901 gggtcgtcgg ccatgacgct gaggccgacc acgcgggcgc gcgcgtcggg tccgagggtc
      961 cacaggatct cgtcgctgag caccgtctgc gagagcacgc ggcgggccgg gccggacgtg
     1021 gtcgagaccg gcggggcgca ggcgacgagc gcgagcaggg ggacgagcag gcggcgcatg
     1081 gctcagggga tcggcacggc ggcgtagggg gcgaggccgg tcgagatcgg caggccgtcg
     1141 aagcgggggg tcggcggggt ggtgcgcagg tcccaggcac gcatgccggc ggcggcgtgg
     1201 ctgcgatcgc cgaaccacag ggtgtcgtcg acgacctcga gggtcgggcc gatcgactgc
     1261 atgccgccgg cgatctcctc gatcggggcg tcgtcgtcga ggctggcgcg cagcagcacg
     1321 acctcgctga ggtcggcgcg gtagctggcg atgtaggcgt gcgcgccggt gcggtcgagg
     1381 tcgaaggcct gggtgaggtc ggtggcgacg aggcccatcg cggcgaggcg cgcggggtcg
     1441 acggcccagg tcgatcgcag ggtcgtgagg tcgaggcgct cgaggccgct gctgaggacc
     1501 aggagggtgt ggccggcggg gtcggcgggg tcggggcgcc actgcttggc ccaggggccc
     1561 agcagcggcg tcgcctgcac gccgggcgcg tcggggtcga ggtcgtgcag gcggccgctg
     1621 gggaggtcga tcggcgcgag gtggtcgtgc gggccggtcg cggcgaagcc gaggttctca
     1681 tcgaggcgct ggacggtgat gaagagggtg tcgccgcagc gaatagcgag gttggcctcg
     1741 gggttgccgt cggggtcggc gagcgcggtg aggtcgatgc tgccggcgtg ggtcggcgcg
     1801 gcggggtcgg tgaggtcgac gatctgcacc gccggggcgc cgaacatcgt gacgtaggcg
     1861 cggtcgcccg cgaaggcgag cgcgtgcggg ttgctgctga cgacgtcggg tacgccgagg
     1921 ccgagctggg ccagcaagcc gaggtcgggg gtgagcacgt cgagggcgtc gaaaccgtag
     1981 cggtgcagca ggtacaggcg cgcgccgtcg tggaacggca cggagtcgct ggtcgcgagc
     2041 gcgaggtcgg gggtgaccgc gccggtgcgc aggtcgagga tgctgagcgc gcccgtgacg
     2101 ttgtcggtgg tcgtgatcgc caggcgggtc ggggccgcga gctcggcggt gcacggctcg
     2161 tcgagcaccg gcgggccggg cagggcgggc tcgcccgtgc tgtccgcgct gccggtcgtg
     2221 tcgtcgacgt ccggggcggt gcaggcgagc acgaggctgg ccgaaaggac aggtgcgagg
     2281 gcgcgcggat ggttcatggg atcgccagga cgaggtaggg gtcgccgggg gtcgagacgg
     2341 cggcgagctc gctgtcgtcg tcgaggttga agcggcgcag gcgcggctgg tccggcgcgg
     2401 cgtcgccgac ccacagcgtg gcgccgacgc gctcgagggt cttctcgcgc gaggtcaggc
     2461 cgtcgacgag cacctgcggg gtcgcggggg cgaggccgtc gaggccgacg tggaagacgg
     2521 cggcgtcggg gtagtcgccg gcggtggcga gcaggtggac gctggcgccg tcggccgcga
     2581 cggtgaaggc ctgcgggtcg tagccgtcga tgccggcggc ggcgagcagg gtggggtcga
     2641 cggcccagcg agatgtcccg aggggcaggt cgacgcgctc gacgccggag gtcaggacga
     2701 gcacggtgtg accgctcggg tcggcggggt cgaggcggac ctgcttggcc cacgggccct
     2761 gcagcggcag cgcctgcgtg ccgtcggtgg cgggatcgag gtcgatcgcg gcgccggtgg
     2821 ccaggtcgac ggcgacgagc cagctgaggt cgaccggcgc gaagtcgacg agccgctgga
     2881 tcgcgacgaa ca
//
LOCUS       DASWNJ010001003         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_511068, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001003 DASWNJ010000000
VERSION     DASWNJ010001003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511068"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..973)
                     /locus_tag="VGB85_16975"
     CDS             complement(44..973)
                     /locus_tag="VGB85_16975"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEY0135782.1"
                     /translation="MARLEDILEQGDVNEVHALSRVELERLFWKYKGLRKQVERDTAF
                     WQATNDTLKTAYERLDEQERELARAYQTIRQDLEIAQQVQHALLPEQFPDMLAQLELA
                     VYHKQLTEVGGDYYDFFRTRAGAYAIGLFDISGHGVSAALIMAFLKAQFMQTMEQVER
                     PKDIVERVNAASLDFLRVVRRYATVNFVVFAESSIRYVCGGGYGVLVRGGEIHNFSKG
                     DPYIGLRRRPYTEFELSFYAGDLLALYTDGMVEGQNAEGQDYGSRRLNGLLCAHASEP
                     IADILARCVDDYREFRRSDSDDITLILLRRRVA"
     gene            complement(1014..1553)
                     /locus_tag="VGB85_16980"
     CDS             complement(1014..1553)
                     /locus_tag="VGB85_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135783.1"
                     /translation="MSASVDTEPMVHGTLMAGFAQACINLNIVAGRQVNDLLSDLDVM
                     QWYPLRRWFQLEQLIGATYQHIDPIRLKLGIEMMTLWYHRGPGREVVRRGVDFLHFQT
                     GSTGIVSVIRGPQGLVGSFDLREFDAAAGRAVIHSTTPFHRKIESGVLIGGLLAPGDL
                     DYVDVHNDGDPDLLVVEFH"
     gene            complement(1550..>2414)
                     /locus_tag="VGB85_16985"
     CDS             complement(1550..>2414)
                     /locus_tag="VGB85_16985"
                     /inference="COORDINATES: protein motif:HMM:NF015478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GRAS family protein"
                     /protein_id="HEY0135784.1"
                     /translation="DLLRKAIPVLPQAYAIANQHLVHTLRGIRSAALLDIGIGKGAQV
                     VTILRTLGAEPGALRRLRIVALDPSASNLAEAKSAIEAAVSQMPGLPFTVEIAQLRGL
                     IERWEPAALQHTIGAVDGVAINAAYALHHTTHPPNDQQARTDLLRRLAGLRPRVFTLV
                     EPNANHDTELLTKRVHSCWQHFGAVFDLVDRSDASPREKLAIKDKFFGREVRDILGTS
                     DTFRCERHEPYESWLLRLTKAGLKPHPPIKLDITLPDYCWATEREGLVRLGYRDQALI
                     AAFAYCSPEAS"
BASE COUNT          366 a          808 c          850 g          390 t
ORIGIN      
        1 acccgcaggg acaccccgac gcgcgtgctc gggccgacat ggctcacgcg acccgccttc
       61 gcagcaagat gagcgtgatg tcgtcgctgt cgctgcgccg aaactcgcgg taatcgtcga
      121 cgcaacgggc gaggatgtcg gcgatcggct cggaggcgtg ggcgcacagc agcccgttga
      181 ggcggcgcga gccgtagtcc tggccctcgg cgttctggcc ctcgaccatg ccgtcggtgt
      241 acagcgcgag caggtcaccg gcgtagaacg acagctcgaa ctcggtgtac ggacggcgcc
      301 gcagaccgat gtacgggtcg cccttgctga agttgtggat ctcgccgccg cgcaccagga
      361 cgccgtagcc tccgccgcaa acgtagcgga tcgacgactc cgcgaaaacc acaaagttga
      421 ccgtggcata acgccgaacg acgcgcagga agtcgagcga ggccgcgttg acgcgctcga
      481 cgatgtcctt gggccgctcg acctgctcca tcgtctgcat gaactgcgcc ttgaggaacg
      541 ccatgatcag cgcggccgag accccgtggc cggagatgtc gaacaggccg atcgcatacg
      601 cgcccgcgcg ggtgcgaaag aagtcgtagt agtcgccgcc gacctcggtc aactgcttgt
      661 gatagaccgc gagctcgagc tgcgccagca tgtccgggaa ctgctcgggc agcagcgcgt
      721 gctggacctg ctgggcgatc tcgaggtcct ggcggatcgt ctgatacgcg cgcgccagct
      781 cgcgctcctg ctcgtcgagc cgctcgtacg cggtcttgag cgtgtcgttg gtcgcctgcc
      841 aaaacgccgt gtcgcgctcg acctgcttgc gcaggccctt gtacttccag aacaggcgct
      901 cgagctcgac gcgggacagc gcgtgcacct cgttcacgtc gccttgctcg aggatgtctt
      961 ccaggcgtgc catgcgtgcg gtccttcgcg ggagcggttc ggggcctcag ggatcagtga
     1021 aattcgacga cgagcaggtc cgggtcgccg tcgttgtgaa catcgacgta gtcgaggtcg
     1081 ccgggcgcca gcagcccacc tatcaacacg ccgctctcga tcttgcggtg aaagggcgtc
     1141 gtcgagtgga tgaccgcgcg gccggcggcc gcgtcgaact cgcgcaggtc gaacgagccg
     1201 accagcccct gcggcccgcg gatcacgctg acgatgccgg tgctgccggt ctggaagtgc
     1261 agaaagtcga ccccgcggcg cacgacctcg cggccgggcc cgcggtggta ccacagggtc
     1321 atcatctcga tgccgagctt gagccggatc gggtcgatgt gctgatacgt cgcgccgatc
     1381 agctgctcga gctggaacca ccggcgcagc gggtaccact gcatcacgtc gaggtcgctc
     1441 agcaggtcgt tgacctggcg accggcgacg atgttgaggt tgatgcaggc ctgggcgaag
     1501 cccgccatca gggtgccgtg gaccatcggc tcggtgtcga cgctggcgct catgaggcct
     1561 ccgggctgca gtaggcgaac gcggcgatca gcgcctggtc gcggtatccg aggcggacca
     1621 gcccctcgcg ctcggtcgcc cagcagtagt cgggcagcgt gatgtcgagc ttgatcggcg
     1681 ggtgcggctt gagacccgcc ttggtcagtc gcagcagcca cgactcgtac ggctcgtggc
     1741 gctcgcagcg gaacgtgtcg ctggtgccga ggatgtcgcg gacctcgcgg ccgaagaact
     1801 tgtccttgat cgccagcttc tcgcgcggcg acgcgtcgct gcggtcgacg aggtcgaaca
     1861 ccgcgccgaa gtgctgccag cagctgtgca cccgcttcgt cagcagctcg gtgtcatggt
     1921 tggcgttggg ctcgacgagc gtgaacaccc gcggccgcag cccggccagc cggcgcagca
     1981 ggtccgtgcg cgcctgttgg tcgttgggcg ggtgggtcgt gtggtgcagc gcgtacgcgg
     2041 cgttgatcgc gacgccatcg accgcgccga tcgtgtgctg cagcgcggcc ggctcccagc
     2101 gctcgatcag cccgcgcagc tgggcgatct cgaccgtgaa gggcagcccc ggcatctgcg
     2161 agaccgccgc ctcgatcgcg gacttcgcct cggcgaggtt gctcgcgctc ggatcgaggg
     2221 cgacgatccg caggcgccgc agcgcccccg gctcggcccc gagcgtgcgc aggatcgtga
     2281 ccacctgcgc ccccttgccg atcccgatgt cgagcagcgc cgcgctccgg atcccgcgca
     2341 gcgtgtggac caggtgctgg ttggcgatcg cgtaggcctg cggcagcacc gggatggcct
     2401 tgcgcagcag gtcg
//
LOCUS       DASWNJ010001004         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_330476, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001004 DASWNJ010000000
VERSION     DASWNJ010001004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330476"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..>1611)
                     /locus_tag="VGB85_16990"
     CDS             complement(126..>1611)
                     /locus_tag="VGB85_16990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY0135785.1"
                     /translation="GEEKGALTFRANYYYSGGFDALGAAFRWLARPMDPFMAMHLLSA
                     MLGVLGVVGTWRLGRRLGGPFVGLVAGLLLAFNPVYFGHMFNNPKDLPFAVAYVWAIH
                     AMVGVIAALPRPTRAQWGSVAVTIAAAMSVRIGGVLLLCYLGLIVALYAFAQGRARRS
                     VEAGLEYLWRIGGRALLTALGAWVLMLMTWPWALLDPLRRPFIALTHMSKFLMHKRLM
                     PFKGEDISTFDIGRDYLPTYFGIQLPEVVLLFGLLGTVYGVYTLARHGHRQRVLVPAL
                     ALLLLGLSIWLPPIYAIYKKSVLYDAYRHFLFVVPPLSVLAALVVDRLAAPLCRRSPR
                     LGAGLVGGVLLAVCLDLGVTMHRLHPHEYIYFNRLVGGLGGAHGNYDTDYYGNTYKEA
                     FTGLRDHLWREDPAAYLDTMYYFRGCISGYTASHYLPPNFRIHRTRPGGKRSADFHVG
                     YTRGHCDQRFANSPVLFTVEREGAALNVVRDLRPGARRRLEGAP"
BASE COUNT          276 a          545 c          590 g          200 t
ORIGIN      
        1 ggcgaggtgg ccggccgcga gcgcgccgtc gcaggtcggg tcgagggcga tcgcccgcgc
       61 ggctcgcagc tcgcgctcgg cgaccgcgag cgcctgctcg tcacccgcgg ggttcggcgc
      121 ttgcgtcatg gcgcgccctc caggcgacgg cgggcgccgg gtcgcaggtc gcggacgacg
      181 ttgagcgcgg cgccctcgcg ctcgacggtg aacagcaccg gcgagttggc gaaccgctga
      241 tcgcagtggc cgcgcgtgta gccgacgtgg aagtcggcgc tgcgcttgcc tcccgggcgt
      301 gtgcggtgga tgcgaaagtt cgggggcaga tagtgactgg cggtgtaacc gctgatgcag
      361 ccgcggaagt agtacatggt gtcgaggtag gccgccggat cctcgcgcca cagatggtcg
      421 cgcagcccgg tgaaggcctc cttgtaggtg ttgccgtagt agtcggtgtc gtagttgccg
      481 tgggcgccgc cgaggccgcc gaccaggcga ttgaagtaga tgtactcgtg cgggtgaagg
      541 cgatgcatcg tcacgccgag gtcgagacat accgcgagca gcacgccgcc gaccagcccg
      601 gccccgaggc gaggcgatcg ccggcacagc ggcgcggcga gccggtcgac caccagcgcc
      661 gcgagcacgg acagcggcgg cacgacgaac aggaagtggc ggtacgcgtc gtagagcacc
      721 gacttcttgt agatcgcgta gatcggcggc agccagatcg acagcccgag cagcagcagc
      781 gccagcgccg gcaccagcac gcgctgccgg tgaccgtggc gcgcgagcgt gtagacgccg
      841 tagacggtgc cgagcagccc gaacagcagc acgacctccg gcagctggat gccgaagtag
      901 gtcgggaggt agtcgcggcc gatgtcgaag gtcgagatgt cctcgccctt gaacggcatc
      961 aggcgcttgt gcatcaggaa cttgctcatg tgcgtcaggg cgatgaaggg ccggcgcagc
     1021 gggtcgagca gggcccacgg ccaggtcatc agcatcagca cccacgcgcc gagggccgtc
     1081 agcagcgcgc gcccgccgat ccgccacagg tactcgaggc cggcctcgac gctgcggcgc
     1141 gcgcgaccct gggcgaacgc gtacagcgcg acgatcaggc cgaggtaaca cagcagcagc
     1201 acgccgccga tccgcacgct catcgccgcg gcgatcgtca ccgcgaccga tccccactgc
     1261 gcgcgcgtcg gacggggcag ggcggcgatc acaccgacca tcgcgtggat cgcccacacg
     1321 tacgcgaccg cgaagggcag gtccttcggg ttgttgaaca tgtgcccgaa gtagaccggg
     1381 ttgaacgcca gcagcagccc ggcgaccagg ccgacgaagg gaccgccgag ccgtcgcccg
     1441 agccgccagg tgccgacgac cccgagcacg ccgagcatcg cgctcagcag gtgcatcgcc
     1501 atgaacgggt ccatcggccg cgcgagccag cgaaacgccg cgccgagggc gtcgaagccc
     1561 ccgctgtaat aataattggc gcgaaaggtc agcgcgccct tctcctcccc g
//
LOCUS       DASWNJ010001005         4299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_18715, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001005 DASWNJ010000000
VERSION     DASWNJ010001005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18715"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1014..2474
                     /locus_tag="VGB85_16995"
     CDS             1014..2474
                     /locus_tag="VGB85_16995"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0135786.1"
                     /translation="MTPGDTTTDGAWIGQTIDGRYQIRELLGEGGMGAVYAAEHLSLR
                     KQVALKVIHPEFAGNAEIAARFTREAMATAQLDHPNVASALDYGTLPGGGAYLVTQLV
                     RGRSLESHRAAGRMQWRQVCDLGSQIADALTAAHAIHIVHRDLKPDNILLEPRDDGTF
                     LVKVLDFGVARITAGANLPTSDLTRMGTVIGTPGYMAPEQAMGESVDFRVDIYALGVI
                     MWECIAGKVLWDGASVSELFTTQLAGPAPPLANEVPGLPPELSALVDLMLDRNPRRRP
                     ESARQIRDALRRMAHSTLIASPAHKPAAAAAAVANPFPRWVLPVSIAVLVALVVIGLT
                     MGQGKTAQRPSHTTDTSTVTAPAAAAGRVEAAPADSDVPAELAGPLRALLGPGDAKAR
                     KAAADAILDHEPREGVALYARNVAWLERANTCEARKAVVKKIAEDGDARALPALQLLS
                     KSSRKGCGLFKNTDCNECLRDPLRRTIDQLSRSGAD"
     gene            2568..>4299
                     /gene="pepN"
                     /locus_tag="VGB85_17000"
     CDS             2568..>4299
                     /gene="pepN"
                     /locus_tag="VGB85_17000"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEY0135787.1"
                     /translation="MFEDQAIATTEPNSPRPIYRTDYRRPDFEVDQVTLRFVLGETET
                     VVHATLAIRRAEGAAADAPLVLDGESLELRELAIDGQALAPDAYAGTPETLTIPRVPA
                     AFELRSVVVIHPETNTELTGLYRSSGNFCTQCEAEGFRRITYFLDRPDVMSRYSVTVE
                     AEKAKYPVLLSNGNRVDGGELADGRHFARWDDPFPKPCYLFALVAGDLRCVGDEFVTR
                     SGRTARVEVWVEPENLDRCAHAVKAAKWAMKWDEDTFGREYDLDVYMIVAVNDFNMGA
                     MENKGLNIFNAKYVLARPDTATDDDYEAIATVIAHEYFHNWTGNRVTCRDWFQLTLKE
                     GLTVLRDRLFSADIGSAAVSRIANVLQLRTSQFAEDRGPMAHPIRPESYIEMNNFYTA
                     TVYEKGAEVIGMLRTLLGVAGFRKGMDLYFERHDGQAVTCDDFVAAMADANHRDLEQF
                     RRWYSQVGTPVVRARGQHDPGGQRFILTLEQAPPHGRDSAEPLHIPVAVGLLGHDGQP
                     LPMQLDGETDASSETRVLELREHSQSFVFTNIVAPPVVSLLRGFSEPFELEFARSDDD
                     LELLMASDPDS"
BASE COUNT          669 a         1558 c         1458 g          614 t
ORIGIN      
        1 cgcatcatca tgatccaact accttgatga cgcgctcgcg ccgcccaacc cccttgtcgg
       61 cgtcaaaatt tcagatcaac ggccagtgcg acgcgggtcc tcccagggga catgctcgcc
      121 gccccatctg tcgcgcataa catctcgctt acgccaagat tgtctctcac gaccggaagt
      181 tgacggggcg tctgcgcgtg acccctgggt cctcccgctg cttcccccgc tcgtcggccc
      241 taccccccgg gcgagcatcg tcggcgcccc gcttgttcac ggggtcatat cgtcgctcga
      301 gtggagggcg tcggcgtggc gtcaccccct actgatctgt gcgcgcgatg ctcgtggtcg
      361 tgctcgagag acttgcaaag tcgcgggtcc atcatccgac tgctgtcgtg cactgtcggg
      421 ggccacgtgc ctcgccgtgg cgcaaagtcg tcgtgcgtcg gggttcgtcg cgtctcgtgt
      481 gctgctggtc cgcgcgggac aagctcgctc gagcaggagt gtcagcggtc acgcacgcga
      541 tgctgtgtgc gtttcgagga gcggggcgca gacgatgtcc gcccggggtt gggggccgac
      601 gagcggggga agcagcggga ggacccaggg gtcacgcgca gacgccccgt caaccgtcgg
      661 acatgcgctc aagggtcggt gactgtgacc gggttatcgg gccagatggg gcggcgagca
      721 ggtcccctgg gaggacccgc gtcgcaggga cccgccgtcc gtcggcccct ccccggccca
      781 gatatcctcg ccccgcgttc tccggaccgc caagccatag ccgggcccgc caagccagag
      841 caggtccgca agccatagcc gggcccgcca agccatagcc gggcccgcca agcgaagccg
      901 ggcccgccaa accggagccc gcccccggga accgcagatc ggctgcaaaa gcccgccagc
      961 acgtaaaagg ccgggtggta ccggcgaacg cgagcgcgta tactcgcgcc cgcgtgacac
     1021 ccggagacac cacgacggac ggggcctgga tcggccagac gatcgacggc cgctatcaga
     1081 tccgcgagct gctcggcgag ggtggcatgg gcgcggtgta tgccgcggag catctgagcc
     1141 tgcgcaagca ggtcgcgctc aaggtcatcc acccggagtt cgccggcaac gccgagatcg
     1201 cggcccgctt cacgcgcgag gcgatggcga cggcccagct cgatcacccg aacgtcgcca
     1261 gcgcgctcga ctacgggacg ctgcccggtg gcggcgcgta cctcgtcacg cagctggtcc
     1321 gcgggcgcag cctcgagagc catcgcgccg ccgggcgcat gcagtggcgg caggtctgcg
     1381 acctcggctc gcagatcgcc gacgcgctga ccgccgcgca cgcgatccac atcgtgcatc
     1441 gcgacctcaa gccggacaac atcttgctcg agccgcgcga cgacgggacc ttcctcgtca
     1501 aggtcctcga cttcggcgtc gcgcggatca ccgccggcgc caacctgccg accagcgacc
     1561 tcacgcgcat gggcaccgtc atcggcacgc ccggctacat ggcgcccgag caggcgatgg
     1621 gcgagtcggt ggactttcgc gtcgatatct atgcgctcgg cgtcatcatg tgggagtgca
     1681 tcgccggcaa ggtgctgtgg gacggcgcga gcgtgtcgga gctgttcacc acgcagctcg
     1741 ccggaccggc gcccccgctc gccaacgagg tgcccggcct gccgccggag ctgagcgcgc
     1801 tggtcgacct gatgctcgac cgcaaccccc ggcgccgccc ggagtcggcg cgccagatcc
     1861 gcgacgcgct gcgtcgcatg gcccacagca cgctgatcgc atcacccgcg cacaagcccg
     1921 ctgccgccgc cgctgcggtc gccaacccgt tcccccgctg ggtcctgccg gtcagcatcg
     1981 ccgtgctcgt ggcgctggtg gtgatcggcc tgacgatggg tcagggcaag acggcgcaac
     2041 gcccgtctca tacgaccgac acctcgacgg tcacggcgcc agctgccgct gccggtaggg
     2101 tcgaagccgc gcctgcggac tccgacgtgc cggccgagct cgcgggtccg ctgcgagcgc
     2161 tgctcgggcc cggcgacgcg aaggcccgca aggccgccgc ggacgcgatc ctcgaccacg
     2221 agccccggga gggcgtcgcg ctgtacgccc gcaacgtcgc gtggctcgag cgcgccaaca
     2281 cctgcgaggc ccgcaaggcc gtggtcaaga agatcgccga ggacggcgat gcgcgcgccc
     2341 tgccggcctt gcaactgctg agcaagagct cgcgcaaggg ctgtggtctg ttcaagaata
     2401 ccgactgcaa cgagtgcctg cgggacccgc tccggcgcac gatcgaccag ctgagccggt
     2461 ccggcgcgga ctgacgggcg ggtccggggc tgctcacggg gcggggtggc ccgggcgggt
     2521 cacgcagacg cttgtcgagg agcgccgggg tcggtacacc ggagttcatg ttcgaggatc
     2581 aggcgatcgc caccaccgag ccgaactccc ctcgcccgat ctaccgcacg gactatcgtc
     2641 ggcccgactt cgaggtcgat caggtcacgc tgcgcttcgt gctcggcgag accgagacgg
     2701 tcgtgcacgc gacgcttgcg atccgccgtg cggagggtgc cgccgccgac gcgccgctcg
     2761 tgctcgacgg cgagtcgctc gagctgcgcg agctggcgat cgacggccag gcgctcgccc
     2821 ccgacgccta cgccggcacg ccggagacgc tgacgatccc gcgcgtcccc gcggccttcg
     2881 agctgcggtc cgtcgtcgtc atccacccgg agaccaacac cgagctgacc ggcctttatc
     2941 gctcgagcgg caacttctgc acgcagtgcg aggccgaggg ctttcgcagg atcacctact
     3001 tcctcgaccg cccggatgtc atgtcgcgct acagcgtcac cgtcgaggcc gagaaggcga
     3061 agtatccggt gctgctgtcg aacggcaacc gcgtcgacgg cggcgagctg gccgacgggc
     3121 gacacttcgc gcgctgggac gaccccttcc ccaagccctg ctacctgttc gcgctcgtcg
     3181 ccggcgacct gcgctgcgtc ggcgacgagt tcgtgacccg ctcgggccgg accgcgcgcg
     3241 tcgaggtgtg ggtcgagccg gagaacctcg accgctgcgc gcacgcggtc aaggcggcga
     3301 agtgggcgat gaagtgggac gaggacacgt ttggccgcga gtacgacctc gacgtgtaca
     3361 tgatcgtcgc ggtcaacgac ttcaacatgg gggcgatgga gaacaagggg ctcaacatct
     3421 ttaacgccaa gtacgtgctg gcgcgccccg acaccgccac cgacgacgac tacgaggcga
     3481 tcgccacggt gatcgcccac gagtactttc acaactggac cggcaaccgc gtcacctgcc
     3541 gcgactggtt ccagctgacg ctcaaggagg gcctgacggt cctgcgcgac cgcctgttta
     3601 gcgccgacat cggctcggcc gcggtgtcgc ggatcgccaa cgtgctgcag ctgcgcacct
     3661 cgcagttcgc cgaggaccgg ggaccgatgg cccacccgat ccggcccgag tcgtacatcg
     3721 agatgaacaa cttctacacc gcgacggtgt acgagaaggg cgccgaggtg atcggcatgc
     3781 tgcgcacgct gctcggcgtc gccggctttc gcaagggcat ggacctctac ttcgagcgcc
     3841 acgacggcca ggccgtcacc tgcgacgact tcgtcgccgc gatggccgac gccaaccacc
     3901 gcgacctcga gcagtttcgc cgctggtaca gccaggtcgg cacgcccgtc gtccgcgccc
     3961 gcgggcaaca cgaccccggc ggccagcgct tcatcctcac gctcgagcag gccccgccgc
     4021 acggccgcga cagcgccgag ccgctgcaca tcccggtcgc ggtcggcctg ctcggccacg
     4081 acggccagcc gctgccgatg cagctcgacg gcgagaccga cgcgagcagc gagacccgcg
     4141 tgctcgagct gcgcgagcac agccagagct tcgtgttcac gaacatcgtc gcgccgccgg
     4201 tcgtgtcgct gctgcgtggc ttctcggagc cgttcgagct cgagttcgcg cgcagcgacg
     4261 acgacctgga gctgctgatg gcgagcgacc ctgactcgt
//
LOCUS       DASWNJ010001006         5987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_266459, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001006 DASWNJ010000000
VERSION     DASWNJ010001006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5987
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266459"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VGB85_17005"
     CDS             complement(<1..543)
                     /locus_tag="VGB85_17005"
                     /inference="COORDINATES: protein motif:HMM:NF018675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HEY0135788.1"
                     /translation="MAGTHRRLGPDPQVWLDRVLAAPAPYSFLAVVDLLERLFPGPAP
                     LGSVIEPQHERIRLQHSSALMCDIGEVVSVELRGEPPQPTLTLSLLGLCGSATPLPLY
                     MAEEADLDDEQGAAIRGLLDILHHRVLSLLVLGLRAVDLPSSLRPDGTDPWSRRVLAF
                     LGQDESSAAQRLPRATLLRLA"
     gene            complement(519..2240)
                     /gene="tssF"
                     /locus_tag="VGB85_17010"
     CDS             complement(519..2240)
                     /gene="tssF"
                     /locus_tag="VGB85_17010"
                     /inference="COORDINATES: protein motif:HMM:NF017733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HEY0135789.1"
                     /translation="MNLSEYYASELTFLRETARAFAQEHPALASSLQERGTDPDVERL
                     LEGVAFMTAGLRARIDQAGAHLAHGLAELVLPHYLRSLPASTIVEFAPNQKTLRARQR
                     IAPERPLVARAIDGTVCRFRTCFPVDLWPLKVGEVRLDDSLPRAPRLEVQLELFEGGR
                     VALADPQPLRFYIHHSDAALATTLLLWLTQHLTGISVTGASSIHRLPPTAVRVLPASE
                     SAVVPWPDTAPLGYRSVLEFFALPEKLHFFELSGLHEIALADNRLTLQFELDRPPPLP
                     GALGPAPLRLHCTPAINLFEAPGEAVSRGPLTRESLVRADGVDPRHMEVHEVRSVVAV
                     SRTHGGRRVFVPFARAGHVAGGAAPAFALRRALSPVDRGIDTYLSLIEPAEARSSLDE
                     EVLSLQLLCTNRALPEGLHVGDVQEELRGALYRGYTNITPVSPPVRMALGSEALWRLL
                     SHLALNQRGPADAESLRGLLHLYNFHAAANAQRGRLGARTIEAVRDVRRDAVTRLVRG
                     VPIRTVRTTVELDERAFLGVGHARLFGGVLDDLYASHININVASELRAVLSPSRVEFT
                     WPVRIGD"
     gene            complement(2244..2657)
                     /gene="tssE"
                     /locus_tag="VGB85_17015"
     CDS             complement(2244..2657)
                     /gene="tssE"
                     /locus_tag="VGB85_17015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HEY0135790.1"
                     /translation="MSGGDNRLVRTPGRGLYRRLAGSTVRRSEQEAVVAHLQAILNTH
                     IGESMSSAQLGIIDFADVVHNFPASAQALLHSIRATILQYEPRLRTVSIQPVASSDPL
                     ALAFEISARYVDERRGSLRLRTELSVNGRFNVQGS"
     gene            complement(3067..3873)
                     /locus_tag="VGB85_17020"
     CDS             complement(3067..3873)
                     /locus_tag="VGB85_17020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135791.1"
                     /translation="MAMKLRTTQLAVFQVDADADFIARVADYITRKYGDAIVDFPGYA
                     CLVSELPPSVLLAIVADSVAQGRAYRMTWESTLAGFTSLRFLVAPNFASHPLVWPILT
                     DDEVPPDSRLDELARCLDDDDWEAVEDCYEAEAWNAALLAERAGIAFPPHIEPSSIQI
                     PPGCLECGADLSIADSIAAGARTCCEHCGTIFRVVAVDPLDLEYLEEEPAPPRENEEV
                     AGLEAEDAFDAAEIEDEEDEDGIDEAPDEDLEDIDEELQGEDHAEIADED"
     gene            complement(3889..4806)
                     /locus_tag="VGB85_17025"
     CDS             complement(3889..4806)
                     /locus_tag="VGB85_17025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135792.1"
                     /translation="MSQPSAAIHGTSSAFSNTPPRPCATCARAARRRDPLDRKCIRPG
                     ADNDDRNRALNPYYGPGHADKFLFRLRKGTKHEVYDDALNGRGTAALPRVRVNYGMRR
                     KIGTGTYVYCFGVLLDPSSAGRGVSGWIDVQSFEAVEANYLRSARFKVCKFGSTPPAR
                     DCKWKVSGNRPAWSYLKVTPFRPPKGPRRDPRPTDTRAVKKRLERLKAARDRWIRDYD
                     KHMKAGDYLATDDHVNVCYSLPGNGGKSLGTVPVGTIFCRSGARKPVALYKRRGTKKV
                     ATMWFVFGHYQAGGRKVKGWIAEKALRPR"
     gene            5177..>5987
                     /locus_tag="VGB85_17030"
     CDS             5177..>5987
                     /locus_tag="VGB85_17030"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0135793.1"
                     /translation="MGTVYLAEHVLLGEECAVKVLSRRFAFDPEWVDRFLLEARAAIR
                     IRHDHVVQIKDFACPEPGLVYMVMEAISGESLSDLLKRERAVSWQRALKMADDVASAL
                     EAAHACGIIHRDIKPSNCVRTTSRGDTDFIKVLDFGIAKFAASTADGAGAPRTATGVW
                     MGTAEYMAPEMYRGEPPDGRVDVYALGALVYKLITGETPYRGSHIEVAVEQSQRSPMP
                     PSRRAPERNIPANVDTLVLRALAGDLKSRTPSMTALREEIAAVLTSPAEVSL"
BASE COUNT          969 a         2019 c         2093 g          906 t
ORIGIN      
        1 cgcgaggcgc agcagggtcg cgcggggcag gcgctgggcg gcgctcgact cgtcctggcc
       61 gagaaacgcg agcacgcgcc tggaccacgg gtcggtcccg tcggggcgca gcgagctcgg
      121 caggtcgacg gcgcgcaggc cgaggaccag cagggagagc acgcggtggt gcaggatgtc
      181 gagcaggccg cggatcgcag cgccctgctc gtcgtcgagg tcggcctcct cggccatgta
      241 gagcggcagc ggcgtggccg agccgcacag gccgagcagc gagagggtca gcgtcggctg
      301 cggcggctcg ccgcgcagct cgacggacac gacctcgccg atgtcgcaca tcagcgcgct
      361 gctgtgctgc agacgaatgc gctcgtgctg cggctcgatg acgctgccga gcggcgccgg
      421 gcccgggaac aggcgctcga gcaggtcgac gaccgcgagg aacgaatagg gcgcgggcgc
      481 tgcgagcacc cggtcgagcc agacctgggg gtcggggcct aatctccgat gcgtaccggc
      541 catgtgaatt ccacccgcga ggggctgagc accgcccgca gctcgctggc gacgttgatg
      601 ttgatgtgag aggcgtagag gtcgtcgagg acgccgccga acaggcgggc gtggccgact
      661 ccgaggaagg cacgctcgtc gagctcgacg gtggtgcgga cggtgcggat cggtacaccg
      721 cggaccagcc tggtgacggc gtcgcggcgg acatcgcgga cggcctcgat cgtgcgcgcc
      781 ccgaggcggc cgcgctgcgc gttcgcggcg gcgtggaagt tgtagaggtg cagcaaacca
      841 cgcagcgact cggcgtcggc cgggccgcgt tgattgagcg cgaggtgcga gagcagccgc
      901 cacagcgcct ccgagccgag cgccatgcgc accggcggcg agaccggggt gatgttggta
      961 tatccgcgat acagcgcgcc gcggagctcc tcctgcacgt caccgacgtg gagcccctcg
     1021 gggagcgcgc ggttggtgca cagcagctgc aacgagagga cctcctcgtc gaggctgctg
     1081 cgcgcctcgg ccggctcgat cagcgacaga taggtgtcga tgccgcggtc gaccggcgag
     1141 agcgcgcgcc gcagcgcgaa ggctggggcc gcgccgccgg cgacgtgccc ggcgcgggcg
     1201 aacgggacga acacccgacg gccgccgtgg gtgcggctga cggcgacgac cgagcggacc
     1261 tcgtggacct ccatgtgccg cgggtcgacg ccgtccgccc gcaccagcga ctcgcgcgtc
     1321 agcgggccgc ggctcaccgc ctcgccgggt gcctcgaaca ggttgatcgc cggcgtgcag
     1381 tgcaggcgca gcggcgccgg cccgagcgcg ccggggagcg ggggcgggcg gtcgagctcg
     1441 aattgcagcg tcaggcgatt gtcggcgagc gctatctcgt gcaggcccga gagctcgaag
     1501 aagtgcagct tctccggcaa cgcgaagaac tcgagcacgc tgcgatagcc gagcggcgcg
     1561 gtgtccggcc acggtaccac cgcgctctcg ctcgccggca ggacccgcac cgcggtcggc
     1621 ggcaggcgat ggatcgacga cgcgccggtg accgagatcc ccgtcaagtg ctgggtcagc
     1681 cacagcagga gcgtggtcgc cagcgccgcg tcgctgtgat gaatgtagaa gcgcagcggc
     1741 tgcgggtcgg ccagcgcgac gcggccgccc tcgaacagct cgagctggac ctcgagccgc
     1801 ggggcccgcg ggaggctgtc gtcgaggcgg acctcgccga ccttgagcgg ccacaggtcg
     1861 accgggaagc aggtacgaaa gcggcagacc gtgccgtcga tggcgcgagc gaccagcggc
     1921 cgctccggcg cgatgcgctg gcgggcccgc aaggtcttct ggttgggcgc gaactcgacg
     1981 atcgtgctcg ccggcagaga ccgtagataa tgcggcagca ccagctcggc gagcccgtgg
     2041 gccaggtggg cgccggcctg gtcgatgcgg gcgcgcaggc ccgcggtcat gaaggcgacg
     2101 ccctcgagca ggcgctcgac gtcggggtcg gtgcctcgct cctgcagcga gctggcgagc
     2161 gccgggtgct cctgggcgaa ggcccgcgcc gtctcgcgca gaaaggtcag ctcgctcgca
     2221 tagtattcgg acaggttcat gcgtcacgac ccctggacgt tgaatcttcc attgaccgac
     2281 agctcggtgc gcaggcgcag cgagccgcgg cgctcgtcga catagcgcgc cgagatctcg
     2341 aaggccagcg cgagcggatc gctgctggcc accggctgga tgctcacggt gcgcaggcgc
     2401 ggctcatact ggaggatcgt cgcgcgaatg ctgtgcagca gcgcctgggc gctggccgga
     2461 aagttgtgca cgacgtccgc gaaatcgatg ataccgagct gagcagacga catggattcg
     2521 ccgatgtgtg tgttcaagat cgcctggaga tgcgcgacca cggcctcctg ctcgctgcgc
     2581 ctgacggtgc taccggccag gcggcgatag agaccgcggc ccggggtacg cacgagccgg
     2641 ttgtcgcctc ccgacatcat caaacacctc caacgaaacc acgaaccgct atcgggcgca
     2701 cacgcatcat caggcaaata cttagccgac ggcgaaggtt ccgacgaccc caaatcgatt
     2761 tgggggatgg gacccgcctc gcggcagagc catcaaatgc gccttctgcg cgttgcccgg
     2821 ccctgactcg agcggcgtga cacgacgtgc atccttttcc cttgcgactg aaagggcgaa
     2881 ctcgacgttc gtcagatccg caactccagt tggatattgc acaccccggc aggtcgaaat
     2941 acgtttctgc caatctacga gggcgccccg gtggacggca aggtcctgtc gggctggacc
     3001 gcgactgccc ggcgccgcgc tgcgtgatcg catgtgttgt ctgggcggag catatgcgtc
     3061 ccggggtcag tcctcgtctg cgatctcggc gtggtcctcg ccctgaagct cctcgtcgat
     3121 atcctcgaga tcttcgtcgg gggcctcgtc gataccgtcc tcgtcctcct catcttcgat
     3181 ctcggcggcg tcgaacgcgt cctcggcttc gagcccggcg acctcctcgt tttcccgtgg
     3241 cggggccggc tcttcctcga gatactcgag atcgagaggg tcgacagcga ctaccctgaa
     3301 gatcgtccca cagtgctcgc agcaggtccg ggctccggcg gcgatgctat cagcgatgga
     3361 caggtcggcc ccgcactcga ggcagccggg cgggatctga atcgagctcg gctcgatatg
     3421 cggtgggaag gcgatgccgg cccgctcggc gaggagcgcg gcgttccatg cctcggcctc
     3481 atagcagtcc tcgacggcct cccagtcgtc atcgtccaga catcgggcca gctcgtcgag
     3541 ccgcgagtcg ggcggcacct cgtcgtcggt gaggatcggc cacacgagcg gatggctggc
     3601 gaaattcgga gcgaccagga accgcagcga ggtgaagccg gcgagcgtcg attcccacgt
     3661 catacgataa gcccgaccct gagcgacgct gtccgccacg atggcgagca gcacggaggg
     3721 gggcaactca ctgacgaggc aagcataacc gggaaagtcg acgatggcgt cgccgtactt
     3781 ccgggtgatg tagtcggcga cgcgagcgat gaagtcggcg tcggcatcga cctgaaacac
     3841 ggcgagctgg gttgttcgaa gcttcatcgc catgtcggct ccacggcgct accgggggcg
     3901 tagcgccttc tcggcgatcc aacccttgac cttccgaccg ccggcctgat agtggccgaa
     3961 cacgaaccac atggtggcga ccttcttcgt cccgcgccgc ttgtaaagcg ccaccggctt
     4021 gcgagcgccg gagcgacaga agatcgtgcc caccggcacc gtaccgagcg acttgccgcc
     4081 gttgcccgga aggctgtagc agacattgac gtggtcgtcg gtcgcgagat aatcaccggc
     4141 cttcatatgt ttgtcgtaat cgcggatcca gcggtccctg gccgccttga gccgctctag
     4201 ccgcttcttc accgcacgcg tgtcggtcgg tcgcggatcg cgtctcgggc ccttcggcgg
     4261 ccgaaacggc gtgaccttca ggtatgacca cgctggccgg ttgcccgaga ctttccactt
     4321 gcaatctcgt gctggcggcg tggaaccgaa cttgcacacc ttgaaccgag cgctgcgcag
     4381 gtagttcgcc tccacggcct cgaatgactg gacatcgatc cacccggaga cgccgcggcc
     4441 ggcgctcgac gggtcgagca ggaccccgaa gcaatagaca tacgtccccg tcccgatctt
     4501 gcggcgcatg ccgtagttga cgcggacccg cggaagggcc gcggtaccgc ggccattcag
     4561 cgcgtcgtcg tacacctcgt gcttcgtgcc ctttcgcaga cggaacagga acttatcggc
     4621 gtggccgggg ccgtagtacg gattcagggc ccgattccga tcgtcgttgt cggcgccggg
     4681 tcggatacac tttcggtcca gcggatcgcg gcgcctggcg gcccgggcac atgtcgcgca
     4741 tggccgcggt ggcgtgttcg aaaatgcgct gcttgtgcca tggatcgccg cgcttggctg
     4801 gctcatgcgc caggattcta ggcggcgaaa cttcgactga aaagctcgca gaacacaccg
     4861 tcggcgctgg tgatcgcgcc tatgtgcgca ttcaagccgc gcccggtctg gtcgtcgctc
     4921 ggcctgggcc catccgctca cctcggctcc gcgggaccga cgcgcggata tctcggggcg
     4981 cccaccacca ccccgatcgg agagcgcgcc ctcaccgcgg ccacgcgacg caattttgcg
     5041 ccgccgtccc gggccctcgc atggggggta gaatactcgg gggagggtat tggtgcggac
     5101 aggggatccc acgaacctgg tagggcaggt cctcgagaac cggtacaagc tccgggagcc
     5161 gatcggcgtc ggcggcatgg gcaccgtgta tctcgccgag cacgtgctcc tcggggaaga
     5221 gtgcgccgtg aaagtgctga gccggcggtt cgcgttcgac ccggaatggg tcgacaggtt
     5281 cttgctcgag gcgcgggccg ccattcggat ccgccacgac catgtcgtgc agatcaagga
     5341 cttcgcctgt cccgagcccg ggctcgtcta catggtcatg gaggcgatct caggcgagag
     5401 cctgtcggac ctcctcaagc gcgagcgcgc ggtctcgtgg cagcgcgccc taaagatggc
     5461 cgacgacgtc gcctccgccc tcgaggccgc ccatgcctgc gggatcatcc accgcgacat
     5521 caagccttcg aactgcgtac gcacgacctc gcgcggcgac accgacttca tcaaggtcct
     5581 cgacttcggg atcgccaagt tcgctgcatc caccgccgac ggcgccggtg ctccgcggac
     5641 cgccaccggc gtgtggatgg gcaccgccga atacatggcc cccgagatgt accgcggcga
     5701 gccgcccgac ggccgcgtcg acgtctacgc cctcggcgcc ctggtctaca agctgatcac
     5761 cggcgaaaca ccatatcggg gctcccatat cgaagtcgcc gtcgagcagt cccagcgctc
     5821 gccgatgcca cccagccggc gcgcgcccga gcgcaatatc cccgccaatg tcgacaccct
     5881 cgtgctccgc gcgctcgccg gagatctgaa gtcgaggacg cccagcatga ccgcgctgcg
     5941 cgaggagatc gccgcggtcc tcaccagccc cgccgaggtc tcgctcg
//
LOCUS       DASWNJ010001007         6135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_195790, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001007 DASWNJ010000000
VERSION     DASWNJ010001007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195790"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VGB85_17035"
     CDS             complement(<1..388)
                     /locus_tag="VGB85_17035"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0135794.1"
                     /translation="MSEQTATVAYTARLGQIAGAWVDAPWVEVDAAGTVVRAQSGRPE
                     HPPEVVHDLGRALLLPGMVNAHSHAFQRQIRGATHRRGAHDPSSFWSWREAMYKAANA
                     LDPEAVFTATRGCYREMLRAGITCVGE"
     gene            complement(399..2576)
                     /locus_tag="VGB85_17040"
     CDS             complement(399..2576)
                     /locus_tag="VGB85_17040"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HEY0135795.1"
                     /translation="MLRSPRRTPARPASLAIHTGLGAAALVLALAGCLKVPTDTDAPG
                     SEQAAPKAAKEVLDRYIVALGGEPAVRKIQQRTVDARMTFLPETGCTDADKDCRRDEK
                     IGSFTLQTTADARMYRRTNLDNQIDEQGYDGKQGWQLRGGVLILEDPDEAAISREDAN
                     LHWYLDIEKRGIEIALEPTRTTDHAGQPRTLDGVRWHVKGSPAGDKVLWFDRATGLLS
                     EEVLEDKSSEELLRQWVIYDDYREVDGVKVPHKIRLINQVGARSQEVIFTTQRVDHAA
                     LDASQFGVPNVPPPKRGEDPILADLGKRREAAAAAPRDVQAQVAYARAAWAAGHFEEA
                     ATAAQATLKLDPKEIEALWILAREQVMAGRYKEAETTLARAEKAGARPQLVAAQVAWI
                     RSHQRDFMGVARALDALGPENAGLAGRYRTFVGKPLQVTAPGCQVVLPLSRDVGAPMV
                     EVEIAGEKIMAMLDTGAADVIVDTKLAAKLKLPIRSRTPIGEQGEIGHTQVDTIKLGG
                     ITVTGVPVDVFPSETLAQMSGSQRAASAVIGVRVLEQFQVTYDLPAGQLTLVHGGPKC
                     KKALTAQRSGGSAAMWLHETHFVYVLAQMNGAEGLFLLNTGMQGVDLTATSRSYALAG
                     IGAPPIRRGEPAIVEVAQFSIGDLLKAEGLRGAYGYFEQGESSDQFRIDGMIGLDVLT
                     RRRVTLDFPERKLYFADPGPAATPAPAGKPAAPAAKPAAPAAK"
     gene            2801..3625
                     /locus_tag="VGB85_17045"
     CDS             2801..3625
                     /locus_tag="VGB85_17045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135796.1"
                     /translation="MLSAAPAQARVPRGSAVERPEPAVAAGPCHERIVGELRRGEVSA
                     GPGCELERQIAQRLQHRLARWRFGFGLLERATPGLGEVSLLVSEGRGCTTWPLRQPGR
                     ATLGLYEIKQAGCWVRRYTGPLAITGVLPDGRKLPAIAVVQVQEGRARVDLTRLTVDL
                     DRRGLPGLDGFVRLELGADGWAGEVDLVAMRASLADHHAAAVLRGRGVAALLAVRHPD
                     HPRADRIRALALKATLERQDADFKAVERGELSPYRFLERHAWSPYRRLVAAMVGAP"
     gene            3622..4161
                     /locus_tag="VGB85_17050"
     CDS             3622..4161
                     /locus_tag="VGB85_17050"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEY0135797.1"
                     /translation="MKSVPGDMSGAVARLRVHLAIIDGGVAPVVARYRAHIGCRAGCS
                     ECCHQSFRVSEVEGALLREGLAAAPAPVRAQIVARAGEYEAGRGEACPVLGDDGNCRL
                     YDHRPRICRKYGIPLWNPERPHELNTCRLNFRGVEDLDAELIVEPQAAWAEDWILLRE
                     QLGFGRQDNRAIAEWLRED"
     gene            complement(4349..5506)
                     /locus_tag="VGB85_17055"
     CDS             complement(4349..5506)
                     /locus_tag="VGB85_17055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135798.1"
                     /translation="MTGLAIVGFGGPAGCQTQHSPGFQKVRPERTAAAAPAAARPLPE
                     GTAEGVPEHLLHEYAGRDLAPDAEIRTWDLPTKAKRVVVELLILAARDDASRLNTVMT
                     QQARWGAPDRREYDARSVSDDGKVFLDTLRQASSRFARKQNLTCPPIMPPAAQVYVRN
                     GSEPMWCFYGSNDGLDILAFKLVVEGGSAKIDYVGMYAERPAHMIPRTGSQPPPMTPM
                     VRRGPRMPNPNMPGLDGQLGDPNALPGQSDAGGVIINNGQPGAPQPGTGADAGQPGGQ
                     PGQPGQPAGSPDQPGAPAGTAAGQHAGSTAGQPAGTAAPRATTGNASPPPGNIRGQAA
                     GATASETTNSPGDPTRKPAPAKPAAPRPGTPKATEPSGTAAQPAGTTSPGQ"
     gene            5757..>6135
                     /locus_tag="VGB85_17060"
     CDS             5757..>6135
                     /locus_tag="VGB85_17060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135799.1"
                     /translation="MAVVPGPRRRVLAWAALWVGVVIASGCPGRPEVVGTPPAAKQSA
                     AGSMVPAATHEAKLPAAHVATPEPTTDPPRDPPPDPTTPAVAPGAGDPRAVPPGTPPA
                     IARVFQRLPVAIHDGPPLGGIGAT"
BASE COUNT          712 a         2113 c         2426 g          884 t
ORIGIN      
        1 actcgccgac gcaggtgatg cccgcgcgca gcatctcgcg gtaacagccg cgcgtcgccg
       61 tgaacacggc ctcggggtcg agcgcgttgg cggccttgta catcgcctcg cgccagctcc
      121 aaaagctcga ggggtcgtgc gcgccgcggc ggtgggtcgc gccgcggatc tggcgctgga
      181 aggcgtggct gtgggcgttg accatgcccg gcagcagcag cgcgcggccg aggtcgtgca
      241 cgacctccgg ggggtgctcg ggccggccgg actgcgccct cacgacggtg ccggcggcgt
      301 cgacctcgac ccacggcgcg tcgacccagg ccccggcgat ctgcccgagc ctggcggtgt
      361 acgcgacggt agctgtctgt tcggacatgt cctcggggtc acttggctgc gggagcggca
      421 ggcttggctg cgggagcggc gggcttgccg gccggagccg gagtcgcggc cgggcccggg
      481 tcggcgaagt agagctttcg ctcggggaag tcgagggtga cgcggcggcg ggtcaggaca
      541 tcgaggccga tcatgccgtc gatgcgaaac tggtcggagc tctcgccctg ctcgaagtag
      601 ccgtaggcgc cgcgcaggcc ctcggccttg agcaggtcgc cgatgctgaa ctgggcgacc
      661 tcgacgatcg ccggctcgcc gcggcggatc ggtggggcgc cgatgccggc gagcgcgtag
      721 gagcggctcg tcgcggtcag gtcgacgccc tgcatgccgg tgttgagcag gaacagaccc
      781 tcggccccgt tcatctgcgc gagcacgtag acgaagtgcg tctcgtgcag ccacatcgcc
      841 gccgagccgc cgctgcgctg cgccgtcagg gccttcttgc acttcgggcc gccgtgcacg
      901 agcgtcagct ggccggcggg caggtcgtag gtgacctgga attgctcgag cacacgcacg
      961 ccgatcacgg ccgaggcggc gcgctggctg ccggacatct gcgcgagggt ctcgctgggg
     1021 aacacgtcga cgggcacgcc ggtgacggtg atgccgccga gcttgatcgt gtcgacctgg
     1081 gtgtggccga tctcgccttg ctcgccgatc ggcgtgcgcg agcggatcgg cagcttgagc
     1141 ttggcggcga gcttggtgtc gacgatgacg tcggccgcgc cggtgtcgag catcgccatg
     1201 atcttctcgc ctgcgatctc gacctcgacc atcggcgcgc cgacgtcgcg ggacagcggg
     1261 agcacgacct gacagcccgg cgcggtcacc tgcaagggct tgccgacgaa ggtgcggtag
     1321 cggccggcga ggccggcgtt ctccgggccg agggcgtcga gcgccctggc gacgcccatg
     1381 aagtcgcgct ggtggctgcg gatccacgcg acctgggcgg cgaccagctg cgggcgggcc
     1441 ccggccttct cggcgcgcgc cagggtggtc tcggcctcct tgtagcgccc ggccatcacc
     1501 tgctcgcgcg cgaggatcca cagcgcttcg atctccttcg ggtcgagctt gagggtcgcc
     1561 tgcgcggccg tcgccgcctc ctcgaagtgg cccgcggccc acgccgcgcg cgcgtaggcg
     1621 acctgagcct gcacgtccct gggagccgcg gcggcggcct cgcgccgctt gccgaggtcg
     1681 gcgaggatcg ggtcctcgcc gcgcttgggc ggcggcacgt tgggcacgcc gaactggctg
     1741 gcgtcgagcg cggcgtggtc gacccgctgc gtcgtgaaga tgacctcctg cgagcgagcg
     1801 ccgacttggt tgatgaggcg gatcttgtgc gggaccttga cgccgtcgac ctcgcggtaa
     1861 tcgtcgtaga tcacccactg gcgaaggagc tcctcgctcg acttgtcctc gagcacctcc
     1921 tcgctcaaga gccccgtcgc gcggtcgaac cacaggacct tgtcgccggc gggggagccc
     1981 ttgacgtgcc agcgcacgcc gtccagcgtg cgcggctggc ccgcgtggtc ggtggtgcgg
     2041 gtcggctcga gggcgatctc gatgccgcgc ttctcgatgt cgaggtacca gtggaggttg
     2101 gcgtcctcgc gcgagatggc cgcctcgtcc gggtcctcga ggatcaacac gccgccgcgc
     2161 aactgccagc cctgcttgcc gtcgtagccc tgctcgtcga tctggttgtc gaggttggtg
     2221 cgccggtaca tccgcgcgtc ggcggtggtc tgcagggtga agctgccgat cttctcgtcg
     2281 cggcggcagt ccttgtcggc gtcggtgcag ccggtctccg gcaagaaggt catgcgcgcg
     2341 tcgacggtgc gctgctggat ctttcgcacc gcaggctcgc cgccgagcgc gacgatgtag
     2401 cggtcgagga cctccttcgc cgccttcggc gcggcctgct cgctgccggg cgcatcggtg
     2461 tcggtcggca ccttgaggca acccgcgagg gcgagcacga gggccgcggc gccgagcccg
     2521 gtgtggatcg caagcgaggc ggggcgggcc ggggtacgtc gaggagaccg gagcatgggg
     2581 tgggagagtg ccgcagccgc gcgcgtggct caaggctcgg caccccacgc caaccgcggc
     2641 caggacggtt ttctttcccg gcgcgccaca aattcccgcg acacctcgcc gccgccacca
     2701 gcgcgcgaca gtgctggtac gctggtccgc ggtgctgcga gcgcgacgac ttcagggaga
     2761 tcggcgaagg cagggtgcgg cgtggctggc cgccgcggtc ttgctgtcgg ccgcgccggc
     2821 gcaggcgaga gtcccacgcg gcagcgcggt cgagcgaccg gagccggccg tcgccgccgg
     2881 gccgtgtcac gagcggatcg tcggggagct gcggcgcggg gaggtcagcg ccggcccggg
     2941 ctgcgagctc gagcggcaga tcgcccagcg cctgcaacac cggctcgccc gctggcgctt
     3001 cggcttcggc ctgctcgagc gcgcgacgcc ggggctcggc gaggtctcgc tgctggtgtc
     3061 ggagggccgc ggctgcacga cgtggccgct gcgccagccg ggccgcgcga cgctcgggct
     3121 gtacgagatc aagcaggccg gctgctgggt gcgccgctac accgggccgc tggcgatcac
     3181 cggggtgctg ccggacggcc gcaagctgcc ggcgatcgcg gtcgtgcagg tccaggaggg
     3241 ccgcgcgcgc gtcgacctga cgcgcctgac cgtcgacctc gatcgccgcg ggcttccggg
     3301 gctcgacggc ttcgtgcgcc tcgagctcgg cgccgacggc tgggccggcg aggtcgacct
     3361 cgtcgccatg cgtgcgagcc tcgccgatca ccacgccgcc gccgtgctgc gcgggcgcgg
     3421 ggtcgcggcc ctgctggcgg tccgccaccc cgatcatccc cgggccgacc ggatccgggc
     3481 gctcgcgctc aaggcgacgc tcgagcgcca ggacgccgac ttcaaggcgg tcgagcgcgg
     3541 cgagctgtcg ccgtatcgct tcctcgagcg ccacgcgtgg tcgccgtacc gccggctcgt
     3601 cgccgcgatg gtgggtgccc cgtgaagtct gtccccgggg acatgtcagg cgcggtcgcg
     3661 cggctgcggg tgcacctggc gatcatcgac ggcggggtcg cgccggtggt cgcccgctac
     3721 cgggcgcaca tcggctgccg cgcgggctgc agcgagtgtt gccaccagag ctttcgcgtt
     3781 tcggaggtcg agggcgcgct gctgcgggag ggcctggccg ccgcgccggc gccggtgcgt
     3841 gcgcagatcg tcgcgcgggc cggtgagtac gaggcgggcc gcggcgaggc ctgtccggtg
     3901 ctcggcgacg acggcaactg ccggctctac gaccaccggc cgaggatctg ccgcaagtat
     3961 gggatcccgc tgtggaaccc ggagcggccc cacgagctca acacctgccg gctcaacttt
     4021 cgcggcgtcg aggacctcga cgccgagctg atcgtcgagc cgcaggcggc ctgggccgag
     4081 gactggatcc tgctgcgcga gcagctcggc ttcggccgcc aggacaaccg ggcgatcgcc
     4141 gagtggctgc gcgaggactg agctggccgt tcggacaggc cgccctgcgg ccccgggaaa
     4201 ggcatctcgg cctgcatcgc ggccgatcgg agcaggccgg gatgcccgcg caaacgcatc
     4261 gtggcctgct ccgaaggcga tcgaagcagg ccgtcgcgcg tccgggacag gtcccggcgt
     4321 cccgaagctg gtcttcggga caggtcgatc actggcccgg cgaggtcgtg ccggccggct
     4381 gcgcggcggt gcccgagggc tccgtggcct tcggcgtgcc cggacgcggc gcggcgggct
     4441 tcgcgggcgc gggcttgcgc gtcgggtcgc cggggctgtt ggtcgtctcg ctcgcagtgg
     4501 cgccggcggc ctggccgcgg atgttcccgg gcggcgggga ggcgttgccg gtggtggccc
     4561 gcggcgcggc ggtgccggcg ggctggcccg cggtcgagcc ggcgtgctga ccggcggcgg
     4621 tgccggcggg ggcgccaggc tggtcggggc tgccggcggg ctgaccgggc tggccgggtt
     4681 ggccgccggg ctgaccggcg tcggcgccgg tgccgggctg cggcgcgccg ggctggccgt
     4741 tattgatgat gacgccgccg gcgtcggatt gaccgggcag ggcgttgggg tcgccgagct
     4801 ggccgtcgag gccgggcatg ttcgggttgg gcatgcgcgg gccacggcgg accatcgggg
     4861 tcatcggcgg cggctgcgag ccggtgcggg ggatcatgtg ggccggccgc tcggcgtaca
     4921 tgccgacgta gtcgatcttg gcgctgccgc cctcgacgac gagcttgaac gcgaggatgt
     4981 cgaggccgtc gttgctgccg tagaagcacc acatcggctc ggagccgttg cgcacgtaca
     5041 cctgcgcggc gggcggcatg atcggcgggc aggtcaggtt ctgcttgcgg gcgaagcggg
     5101 acgaggcctg gcgcagcgtg tcgaggaaga ccttgccgtc gtcgctgacc gagcgcgcgt
     5161 cgtactcgcg gcgatccggg gcgccccagc gggcctgctg cgtcatcacg gtgttgaggc
     5221 gcgaggcgtc gtcgcgcgcg gcgaggatca gcagctcgac gaccacgcgc ttggccttgg
     5281 tcggcagatc ccacgtgcgg atctcggcgt cgggcgcgag gtcgcggccg gcatactcgt
     5341 gcagcaggtg ctcggggacg ccctcggccg tgccctccgg cagcggacgc gccgccgcgg
     5401 gagccgctgc cgcggtccgc tcgggacgca ccttctgaaa gccggggctg tgctgggtct
     5461 gacaccccgc agggccgccg aacccgacga tggcgaggcc ggtcaccaca aggagcgtac
     5521 gcaagggacg ggacgggacg cggagcatgg ccccgtctct tagaccgccg cgcgccattg
     5581 agcaagcgca cgcgatgaga ggatgctcgg ctcgggtgtc ccgcgcgcgg gcgctggcag
     5641 gtggtctgcg tcgcggggtc tccgggcgga gacgcgcggg cggcggttgt tcacgccggt
     5701 caaacctggc taggatgcac gccgcaggcg cgctcgtggc gagatcagga acattcgtgg
     5761 ccgtcgtgcc ggggccgcgt cggcgggtcc tggcctgggc agcgctgtgg gtgggtgtgg
     5821 tgatcgcctc cggctgtccg ggccggcccg aggtcgtcgg gaccccgccg gcggcgaagc
     5881 agtcggccgc gggatcgatg gtgccggcgg cgacccacga ggcgaagctg ccggccgccc
     5941 acgtcgcgac gcccgagccg acgaccgatc ccccgcgaga tcctcccccg gatccgacga
     6001 cgcccgccgt ggcgccgggc gccggagatc cccgggcggt gccgccgggc acacccccgg
     6061 cgatcgcccg ggtgttccag cgcttgccgg tcgcgatcca cgacggtccg ccgctcgggg
     6121 gcatcggcgc gacgg
//
LOCUS       DASWNJ010001008         1675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_688823, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001008 DASWNJ010000000
VERSION     DASWNJ010001008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688823"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..1311
                     /locus_tag="VGB85_17065"
     CDS             259..1311
                     /locus_tag="VGB85_17065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135800.1"
                     /translation="MATVHILRGRDTLRITLSGPRLITGEGDSAIVQAFASEDAARSH
                     LDMVLARRRRSGYTIETQELDEEPALPDPLVGVVEYDATRRNMRVTFKGSEIPPGRCD
                     QIVARAAEVKPLCLQILCDHASPGAALSSALAGVRLPSIRSFIFDTYFQTVTRQAENS
                     PGDLAAVLEAMPALEAAFITGDVLLRPLAHATLRALFLLADPMTPRSISGLGGSHLPA
                     LTTLGVLLAADNGPLPGEPIAGALLKLGAPKLARVEVDGIEDISTFLAALTSKALPAS
                     WSTLRVDGGIADEDALLSVLSERKTKMAKLSTLALPLADEVSQDAAEQGAKLLPSLKD
                     CDDVPTSLLPATYEKW"
BASE COUNT          258 a          578 c          570 g          269 t
ORIGIN      
        1 ggccgctacc gggtcgtggt cgagggcttc ccggacgcgg ggcagacgct ggcgaccggg
       61 gacgtcgccg acgcgctgct gtgcgcgctg caccgcgagg acctgcacgc gcggcgcgtc
      121 ggcgtcgcgt attgatgcgt cgcgcgcgtc gaggcggcga tcgcgtcggg gcctgacgag
      181 cgacgtcgtt tttcgatgtg cgttacgccg cgcacgaggt ggcgaccgcg gacgcccggg
      241 ctggcgtatg caaggcgcat ggcgacggtg catatcctcc gggggcgcga cacgctgcgg
      301 atcactctgt ccgggccgcg gctgatcacg ggcgagggcg actcggcgat cgtgcaggcg
      361 tttgccagcg aggacgcggc ccgctcgcat ctcgacatgg tgctcgcccg ccggcgccgc
      421 tccggctaca cgatcgagac ccaggagctc gacgaggagc ccgcgctgcc cgacccgctg
      481 gtcggcgtcg tcgagtacga cgcaacccgc cgcaacatgc gagtcacgtt caagggctcg
      541 gagatcccgc cgggccgctg cgaccagatc gtcgcccgcg cggccgaggt caagccgctg
      601 tgcctgcaga tcctctgcga ccacgcgagc cccggcgccg ccctgtcgtc cgcgctcgcc
      661 ggcgtgcgcc tgccgtcgat ccgcagcttc atcttcgata cgtacttcca gacggtcacg
      721 cgccaggccg agaactcgcc cggtgacctc gccgccgtgc tcgaggcgat gcccgccctc
      781 gaggccgcgt tcatcaccgg cgacgtgctg ctgcgaccgc tcgcccacgc gaccctgcgc
      841 gcgctgttcc tgctcgccga cccgatgacc ccgcgcagta tctcgggcct cggcggcagc
      901 cacctccccg cgctgacgac cctcggcgtg ctgctggccg ccgataacgg cccgctcccg
      961 ggcgagccga tcgcgggggc gctgctcaag ctcggcgcgc cgaagctcgc gcgtgtcgag
     1021 gtcgatggga tcgaggacat cagcacgttc ctcgccgccc tgacgagcaa ggcgctcccg
     1081 gcctcgtggt cgacgctgcg cgtcgacggc gggatcgccg acgaggacgc gctgctctcg
     1141 gtgttgagcg agcgcaagac caagatggcc aagctgtcga cgctcgccct gccgctcgcc
     1201 gacgaggtgt cgcaagacgc ggccgagcag ggcgccaagc tgctgccgag cctcaaggac
     1261 tgcgacgacg tgccgacgtc gctgctgccg gcgacctacg agaagtggtg aaggaatcgc
     1321 ctggttctca ggcctcacgt gtacggagcg ctcccgcacg tcacgtgact gatgcgatct
     1381 cgcgccacgt cttgattggt agagatcgtt ccgatattct tggggcgatc atgccagact
     1441 tgtccgacat tgtttccttt attctggggc tctcttccgg atttgccatc aagatagcgg
     1501 tcgacgcaag gcggaccacc aagagtgaaa accaaacgtc acagcaagct tctaatcaag
     1561 ctgggggtca tatcgctggc cgcgacgtaa atgttaatgg agaccgtaag ggatgagtct
     1621 tttgaaacca gccgtagtcc aggaaggaaa caccgcgggg gggcatctcg ctggc
//
LOCUS       DASWNJ010001009         2363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_123535, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001009 DASWNJ010000000
VERSION     DASWNJ010001009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123535"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VGB85_17070"
     CDS             complement(<1..344)
                     /locus_tag="VGB85_17070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135801.1"
                     /translation="MLWSPRRRITPGLVLVTLALATACTADKTASTSAPSAMPPAAIV
                     TPEPEPEPTPVAAVDAQPAEPAIAAEPAPEPEVPAVPETPKKVLILGDSLAATGFGAL
                     LERKLDAHPSIV"
     gene            880..1233
                     /locus_tag="VGB85_17075"
     CDS             880..1233
                     /locus_tag="VGB85_17075"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY0135802.1"
                     /translation="MATSAAVMYRVGGNECRSAHRAQVDTSYSYSGIGAVIQGRGDHV
                     IVRSVLPGAPADGMIREGARLISVDGTYPDSVEGWASALKGPEGTSVTVEVAYRCSGH
                     KFVTLERKMIRVTRD"
     gene            complement(1384..>2363)
                     /locus_tag="VGB85_17080"
     CDS             complement(1384..>2363)
                     /locus_tag="VGB85_17080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135803.1"
                     /translation="VLAAGVAGLCVAPRELLWFALSVAVIDGGGVLVGQRLTGGQLWV
                     YIYELHQTHAFNAERFTRKTWGMFLHAGPFVVVMLVGRLVMWARRRLSLGTGWQGRVY
                     WAIVTAAALLVSALGYSTQWAEPNAFMPGICLGSAFVAVMLPVGHGEATALALVGAQL
                     IFSLVLEPRYQPIQDRGLAGLGDSYKMQDPGRTLPTADARARARALREELVARAGPVL
                     ALQRPWWSVLAGGSGHVGTMGLHDVPRTQQRLIEREIAGRVAAGDYVAVYLDGEPPAW
                     LRPALQRRYAVVRSAQGEARVLPLTGYMSEAGMVTPWRGPQRTYVPREP"
BASE COUNT          342 a          903 c          813 g          305 t
ORIGIN      
        1 cacacgatgc tcgggtgggc gtcgagcttg cgctcgagca gcgcgccgaa gccggtcgcc
       61 gccaggctgt cgccgaggat cagcaccttc ttcggcgtct ccggcaccgc gggcacctcc
      121 ggctcgggcg ccggctcggc tgcgatcgcc ggctcggccg gctgcgcgtc gacggccgcg
      181 accggcgtcg gctccggctc cggctccggc gtcacgatcg ccgcgggagg catggccgag
      241 ggggccgagg tcgaggccgt cttgtccgcc gtgcaggcgg tggccagggc cagggtcacg
      301 agcacgagcc cgggggtgat gcggcgccga ggagaccaga gcatgggccg tgcctatatc
      361 gcggaagacc cgcccgagga aagaaccggc aaattggacg tggcagcgcg aaggtgcaga
      421 ggtgtgggtg acgccggggt gcgacgaccg cgcggcagcc tcgcactcgc tggcatcgtc
      481 tcggcgtcgc aacgccgccg ccgcgacccc ggacgatctc ctgcgacacg cagggccggc
      541 ggaaggagcg gcatcggacg tcggtcgcgc cggcgggccc gtctgtcgcg ctgtgtgccc
      601 gtggggcccc ccgcgcccgg accgccgagc accgcggcgg atgtcgcggc tgtcgcggct
      661 gcccgcgtcc gcgcggggga atacgtgcgg cgcaggcaca gtgttagctg gcccctggca
      721 agccacgcca agctgtgaaa cactcgtcat cagtctgtct cggacaggga gcgaaggcca
      781 atgcgttctc ggaacactga atctccatta gcgacttcca tcggccgctg gcgcactgcc
      841 cggcgcgtca ttctcggctc catcgtcctc gcgggcgcga tggccacctc ggcggcggtg
      901 atgtaccgcg tcgggggcaa cgagtgccgt tcggcacatc gtgctcaggt cgacaccagc
      961 tactcgtaca gcggcatcgg tgcggtgatc cagggtcgcg gcgaccacgt catcgtccgg
     1021 agcgtgctcc cgggtgcgcc ggccgacggc atgatccgtg agggcgcccg cctgatctcg
     1081 gtcgacggta cctaccccga cagcgtcgag ggttgggcct cggcgctcaa gggcccggag
     1141 ggcaccagcg tcacggtcga ggtcgcgtac cgctgcagcg gtcacaagtt cgtgaccctc
     1201 gagcgcaaga tgatccgcgt gacgcgggac tgagacgaag cggcctcgct tctcactcgc
     1261 ccgtcgtcgg ccccacgcaa gtggcggccg aggacgcctg cacccgcgaa cctcccgcga
     1321 ggtccccacc atcccgaagg ctccgaccgt gctcgcacga ccggagcctt cggcgttcca
     1381 gcttcacggc tcgcgcggca cgtaggtgcg ctgcggccct cgccacggcg tcaccatgcc
     1441 ggcctcggac atgtagcccg tcagcggcag cacccgcgcc tcgccctgcg cgctgcggac
     1501 caccgcatag cgccgctgca gtgccggccg cagccaggcc ggcggctcgc cgtcgaggta
     1561 caccgcgaca tagtcccccg cggcgacccg ccctgcgatc tcccgctcga tcagccgctg
     1621 ctgcgtccgt ggcacgtcgt gcaggcccat cgtgccgaca tgccccgacc caccggcgag
     1681 cacgctccac cacggccgct gcaacgccag caccggcccc gcgcgcgcga ccagctcctc
     1741 gcgcagcgcc ctcgcccgcg cccgcgcgtc ggccgtcggc agcgtgcgcc ccgggtcctg
     1801 cattttatag ctgtccccga ggccagccag cccgcgatcc tggatcggct ggtacctcgg
     1861 ctcgagcacg aggctgaaga tcagctgcgc gccgaccagc gcgagcgcgg tcgcctcgcc
     1921 gtggccgacc ggcagcatca ccgccacgaa cgcgctgccg aggcagatcc ccggcatgaa
     1981 cgcgttgggc tccgcccact gcgtgctgta ccccagcgcc gagaccagca gcgccgccgc
     2041 cgtcacgatc gcccagtaca cgcgcccctg ccagcctgtc ccgagggaca gccgcctgcg
     2101 cgcccacatc acgagccgac cgaccagcat cacgaccacg aacggccccg cgtgcaggaa
     2161 catgccccag gtcttgcgag tgaagcgctc ggcgttgaag gcgtgggtct ggtgcagctc
     2221 gtagatgtag acccacagct gcccgccggt gaggcgctgc ccgacgagca cgccgccgcc
     2281 gtcgatcacc gcgacgctga gcgcgaacca cagcagctcg cgcggcgcga cgcacagccc
     2341 cgcgaccccg gccgcgagca cga
//
LOCUS       DASWNJ010001010         4330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_476992, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001010 DASWNJ010000000
VERSION     DASWNJ010001010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476992"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2936
                     /locus_tag="VGB85_17085"
     CDS             <1..2936
                     /locus_tag="VGB85_17085"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0135804.1"
                     /translation="AREEDRYEDALVAFEARQNKKDSDPAPTPPRVEYGRSVALFHQL
                     IERFPAYVHSDAALYMLALLRFEEERFDDARQSLLALACASRFPVPPVDGMKAPVRWR
                     RGDYNECTPRRAGSKYVAEAWLRIGEIHYDLDELDPALAAYTEAARDEKGQFYDEALL
                     RIAWTLYLKRDFPGSAVKLDEFVRFVEALKTSDPENSALALRDDAIRYIAKCYVEEDW
                     DLDGNPDAGWGLPRLDRDYKDRGAEPHVPEIYAALGDLFGTQADYKLAITIYDTALRR
                     WPTARAAPALQKKILDAYVALGDGAGTRKARELLAANYLRGTPWFFANEGDTEAIDEA
                     MKLVEDALVASAVERHAYAQSLRAAGDPKATDEYRAAAAAYEAYLLRYPDTPSSYGYR
                     YDYAESLFFSGQYMLAAQAYTAVRDSNTAASRQVDAAEGVVLALEALLDEAQKANSIT
                     MPDLPKQGVAKGPFDPRPIPPLLVALQSAYDRYVGVKPDSKEAGALMFKAGAISQRYY
                     HFDDAEERFVQVVNTHCGDNIAINAGFAIVDGRVVREDLKGAQEWTDKLLEKSCGSGE
                     DSAKFAGDLKTLGNAVRFQEANLLFEAGEFEAAADRYVALVAGAPKDPNADRALNNAA
                     VAYEKIGRFGSASKTYERIYKDYPTSEFADDALLRSGLNHVRFFEFDSAVQSYLVLAE
                     DPRYKDSEHRLIALKNAADLLDNLQQYPKSSELFVRYAGQTKDPKEAVDATFRAAQVL
                     RKTDNSRATEAAFAAFLGKYGSDPKEAAKAVEAQLRIGQARAAMGDRKGAETAFRDCI
                     ATFTSRGLQVNTDAADFPAEAQFLISEYSLADLLEFKLQGTGKKLADNTKILFDRVVL
                     ASKSYDGVLPYRRIDWVLHAMFRRGYAFEITAIKMREAPVPRELKEFSEPWFAYKDMV
                     EQGASKFEAMAIPLYEETIKRAREYGVANEYTRKALERMNIYKPDQYPLLHDPAIELQ
                     VEDRR"
     gene            2933..>4330
                     /locus_tag="VGB85_17090"
     CDS             2933..>4330
                     /locus_tag="VGB85_17090"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0135805.1"
                     /translation="MKRTKWLFVLGLSACTPKPAETAAPESSEPEPEPTEVTPADAGV
                     DPAKAAAAAAVKKKNRDRDPAEEARKMAASRQQSAQASASLRAGSVAAALDLAREALK
                     THEQNVDAMLVMAEAFLKQGKHELTQTVTTSALAVDPKLLTPVETGKIYNLKGFAYLA
                     ADKQNLATQSFRKAAEADARNATAWNNLGAQYLRTGNFKTAAECFTYATKLDPGFYKA
                     QLNLGNARRALGDVQNAELSYRAALNLRANYPEAYFSLGVLYLDASEYPGIDTQTRLT
                     RALNFFGKYRELAIAAGAAAGDRPGDDVARVKTGPKAAKSQKSAPGKEGVSLAQADLY
                     IELAKKGIEREKKREERNQKRGAEAAAAPADPGKAASPADAGKSPATPAADPAAQPAD
                     KPSPAGTPPAATAKPGAPTPSTPPPTAPSTPTAKPGTATPSTPPAAKPGTAPSTPAAK
                     PGTATPSTPPAAKPGT"
BASE COUNT          785 a         1656 c         1354 g          535 t
ORIGIN      
        1 tcgcccgcga ggaggaccgc tacgaggacg cgctcgtcgc cttcgaggcg cgccaaaaca
       61 agaaagacag cgacccggcc ccgacgccgc cgcgcgtcga gtacggccgc agcgtcgcgc
      121 tgttccacca gctgatcgag cgcttcccgg cctacgtgca cagcgacgcc gccctctaca
      181 tgctcgcgct gctgcgcttc gaggaggagc gcttcgacga cgcgcgccag tccctgctcg
      241 cgctcgcgtg cgcgagccgc ttcccggtcc cgcccgtcga cggcatgaag gctccggtcc
      301 gctggcgccg cggcgactac aacgagtgca ccccgcggcg cgccggcagc aagtacgtcg
      361 ccgaggcctg gctgcgtatc ggcgagatcc attacgacct cgacgagctc gatccggcgc
      421 tcgccgccta caccgaggcc gcgcgcgacg agaaaggtca gttctacgac gaggccctgc
      481 tgcgcatcgc ctggaccctt tatctcaagc gcgacttccc gggctcggcg gtcaagctcg
      541 acgagttcgt gcgcttcgtc gaggcgctca agaccagcga ccccgagaac tccgcgctgg
      601 ccctgcgcga cgacgcgatc cgctacatcg ccaagtgtta cgtcgaggag gactgggacc
      661 tcgacggcaa ccccgacgcc ggctggggcc tgccgaggct cgatcgtgac tacaaggatc
      721 gcggcgccga gccgcacgtc ccggagatct acgccgcgct cggcgacctg ttcggcaccc
      781 aggccgacta caagctggcg atcacgatct acgacaccgc gctgcgccgc tggccgaccg
      841 cccgcgccgc gccggccctg cagaagaaga tcctcgacgc atacgtcgcg ctcggtgacg
      901 gcgccggcac ccgcaaggcg cgcgagctgc tcgccgcgaa ctacctccgc ggcacgccat
      961 ggttcttcgc caacgagggc gacaccgagg cgatcgacga ggcgatgaag ctcgtcgagg
     1021 acgcgctggt cgcctccgcc gtcgagcgcc acgcctacgc ccagagcctg cgcgcggccg
     1081 gcgacccgaa ggccaccgac gagtaccgcg ccgccgcggc cgcctacgag gcctacctgc
     1141 tgcgctaccc cgacaccccg agctcgtacg gctaccgcta cgactacgcc gagagcctgt
     1201 tcttctccgg ccagtacatg ctcgcggccc aggcctacac ggcggtgcgc gactcgaaca
     1261 ccgcggcgag ccggcaagtc gacgccgccg agggcgtcgt gctcgcgctc gaggccttgc
     1321 tcgacgaggc gcaaaaggcc aacagcatca ccatgcccga cctcccgaag cagggcgtcg
     1381 ccaagggtcc cttcgacccg aggccgatcc cgccgctcct ggtcgccttg cagtccgcgt
     1441 acgaccgcta cgtcggcgtc aagccggaca gcaaggaggc cggcgcgctg atgttcaagg
     1501 ccggcgcaat ttcccagcgc tactaccact tcgatgacgc cgaggagcgc ttcgtccagg
     1561 tcgtcaacac ccactgcggc gacaacatcg cgatcaacgc cggctttgcg atcgtcgacg
     1621 gtcgggtcgt gcgggaggac ctcaagggcg cgcaggagtg gaccgacaag ctcctcgaaa
     1681 agtcgtgcgg ctccggcgag gactcggcca agttcgccgg tgacctcaag accctcggca
     1741 acgccgtgcg cttccaggag gccaacctgc tgttcgaggc cggcgagttc gaggcggcag
     1801 ccgaccgcta cgtcgcgctc gtcgccggcg cccccaagga ccccaacgcc gaccgcgcgc
     1861 tcaacaacgc cgccgtcgcg tatgaaaaga tcggccgctt cggcagcgcc agcaagacct
     1921 acgagcgcat ctacaaggac tacccgacga gcgagttcgc cgacgacgcc ctgctgcgct
     1981 ccggcctcaa ccacgtgcgc ttcttcgagt tcgacagcgc cgtgcagagc tacctcgtgc
     2041 tcgccgagga cccgcgctac aaggactccg agcaccgact gatcgccctc aagaacgccg
     2101 ccgacctgct cgacaacctc cagcagtacc ccaagtcgag cgagctgttc gtccgctacg
     2161 ccggccagac caaggacccc aaggaggccg tcgacgccac tttccgcgcc gcccaagtcc
     2221 tgcgcaagac cgacaacagc cgcgcgaccg aggccgcctt cgcggccttc ctcggcaagt
     2281 acggcagcga ccccaaggag gccgccaagg ccgtcgaggc ccagctgcgc attggccagg
     2341 cgcgcgccgc catgggcgat cgcaagggcg ccgagactgc gtttcgcgac tgcatcgcca
     2401 cgttcacctc gcgcggcctg caggtcaaca ccgacgccgc cgacttcccg gccgaggcgc
     2461 agttcctgat ctcggagtac tcgctcgccg acctgctcga gttcaagctc cagggcaccg
     2521 gcaagaagct ggccgacaac accaagatcc tgttcgaccg cgtcgtgctc gcctcgaagt
     2581 cgtacgacgg cgtgctgccg taccgccgca tcgactgggt gctgcacgcg atgttccgcc
     2641 gcggctacgc cttcgagatc accgcgatca agatgcgcga ggccccggtg ccgcgcgagc
     2701 tcaaggagtt tagcgagccc tggttcgcct acaaggacat ggtcgagcag ggcgcctcga
     2761 agttcgaggc gatggcgatc ccgctgtacg aggagacgat caagcgggcc cgcgagtacg
     2821 gtgtcgccaa cgagtacacc cgcaaggccc tcgagcgcat gaacatctac aagccggacc
     2881 agtacccgct gctgcacgac cccgcgatcg agctgcaggt ggaggaccgg cgatgaagcg
     2941 cacgaagtgg ttgttcgtgc tcggcctgtc cgcgtgcaca ccgaagcccg cggagaccgc
     3001 cgcaccggag tccagcgagc ccgagcccga gccgaccgag gtcacgcccg cggacgccgg
     3061 cgtcgatccg gcgaaggccg ccgcggccgc ggcggtgaag aagaagaacc gcgaccgcga
     3121 tcccgccgag gaggcgcgca agatggctgc ctcgcgccag cagagcgcac aagcctcggc
     3181 gtccctgcgc gcggggtccg tcgccgcggc gctcgacctg gcccgcgagg ccctcaagac
     3241 ccacgagcag aacgtcgacg cgatgctcgt gatggccgag gcctttttaa aacagggcaa
     3301 gcacgagctg acgcagaccg tcacgacctc ggccctcgcc gtcgacccca agctgctgac
     3361 gcccgtcgag accggcaaga tctacaacct caagggcttc gcctacctcg ccgccgacaa
     3421 gcagaacctc gccacccagt cgttccgcaa ggccgccgag gccgacgccc gcaacgcgac
     3481 cgcgtggaac aacctcggcg cgcagtacct ccgcaccggc aattttaaaa ctgccgccga
     3541 gtgcttcacc tacgcgacca agctcgaccc cggtttttac aaggcccagc tcaacctcgg
     3601 caacgcgcgc agggccctcg gcgacgtgca aaacgccgag ctctcgtacc gtgcggcgtt
     3661 gaacctgcgg gccaattacc ccgaggccta cttctcgctc ggcgtgctct acctcgacgc
     3721 cagcgagtac ccgggcatcg acacccagac ccgcctgacc cgcgcgctca acttcttcgg
     3781 gaagtaccgc gagctggcga tcgcagccgg agccgcagcc ggagatcgcc ccggcgacga
     3841 cgtcgcccgc gtgaagaccg gccccaaggc ggcgaagtcg cagaagtccg cccccggcaa
     3901 ggagggcgtc tcgctggccc aggcagatct ctacatcgag ctcgccaaga agggcatcga
     3961 gcgcgaaaag aagcgagagg aacgcaacca gaagcgcggc gcggaagcag ccgcggcgcc
     4021 ggccgacccc ggcaaggccg cgtcgccggc cgatgccggc aagagcccgg ccacgcccgc
     4081 agcagatccc gcggcccagc ccgccgacaa gccgagcccc gccggtacac cccccgctgc
     4141 gaccgcgaag cccggagcgc cgacaccctc gacacctccg ccgacggcac cgtcgacgcc
     4201 cactgccaag cccgggacag ccactccgtc gacgccgcct gctgccaagc ccgggacagc
     4261 accgtcgacg cctgctgcca agcccgggac agccactccg tcgacgccgc ctgctgccaa
     4321 gcccgggaca
//
LOCUS       DASWNJ010001011         1510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_18824, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001011 DASWNJ010000000
VERSION     DASWNJ010001011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18824"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1408)
                     /locus_tag="VGB85_17095"
     CDS             complement(<1..1408)
                     /locus_tag="VGB85_17095"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY0135806.1"
                     /translation="MTQARAGSFEFDYVIIGGGSAGCVAAGQLSQDPRVRVLLLEQGD
                     AAEQHPETLRADGYKDAFINDSLMYERFSVPQAACLHRPLFMGSGRGMGGSGSINAMV
                     YLRGSAADFRAWDTPGWSWGDVTPDFEVIEKTLDIHRREPTEFTEAAIVAAESAGFHR
                     QQDLNDGDFLGGLGYEWMNYSGDQRRNAYVGFLRPHLDRPNLVVLTGAAVQRLVFDDR
                     RRLTGVEFDHEGARHTARVRREALLCAGALESPKLLMLSGVGPRAQVVGHGIPPVFDL
                     PGVGENFQDHPNVTLFHLARREVDSNYPQVYGFHRANPQSDLPPDQADTCYVVYPARS
                     SFREAFMRMVPALTVPPALYHRTRLPRMVRAGVAMAFKPLMVRQFIRRVYGLVVILGK
                     PKSRGTVRLASRRATDHPIVDPAYFADPEDMDTMIKGVELARRIADGAPLQSFGNREL
                     LPGRWAGGRTAKAAFVRAM"
BASE COUNT          223 a          492 c          583 g          212 t
ORIGIN      
        1 ccatcgcgcg cacgaaggcg gccttggcgg tgcgtccacc ggcccagcga ccgggcagca
       61 gctcgcggtt gccgaacgac tgcagcggcg cgccgtcggc gatccggcgc gcgagctcga
      121 cgcccttgat catcgtgtcc atgtcctcgg ggtcggcgaa gtaggccggg tcgacgatcg
      181 ggtgatcggt ggcgcggcgc gaggcgagcc gcacggtgcc gcggctcttg ggcttgccga
      241 ggatcacgac gaggccgtac acccggcgta tgaactggcg gaccatcagc ggcttgaagg
      301 ccatagcgac gccggcccgg accatgcgcg gcagacgggt gcggtggtac agcgcgggcg
      361 ggacggtcag cgccgggacc atgcgcatga aggcctcgcg gaagctcgag cgcgcgggat
      421 acacgacgta gcaggtgtcg gcctggtccg gcgggaggtc gctctgcggg ttggcgcgat
      481 gaaagccgta gacctggggg tagttgctgt cgacctcgcg gcgcgcgagg tggaaaaggg
      541 tcacgttggg atggtcctgg aagttctcgc cgacgccggg caggtcgaag acggggggga
      601 tgccgtgacc gacgacctgc gcgcgcgggc cgacgccgga cagcatcaac agcttgggcg
      661 actcgagggc accggcgcac agcagcgcct cgcggcgcac gcgggcggtg tgccgcgcgc
      721 cctcgtggtc gaactcgacg ccggtgaggc ggcggcggtc gtcgaacacg agtcgttgca
      781 cggcggcgcc ggtcaacacg acgaggttgg ggcgatcgag gtgcgggcgc aggaagccga
      841 cgtaggcgtt gcgccgctgg tcgccgctgt agttcatcca ctcgtagccg aggccgccga
      901 ggaagtcgcc gtcgttgagg tcctgctggc ggtgaaagcc ggcgctctcg gccgcgacga
      961 tcgcagcctc ggtgaactcg gtcggctcgc ggcggtggat gtcgagggtc ttctcgatca
     1021 cctcgaagtc gggggtgacg tcgccccacg accagcccgg cgtgtcccag gcgcgaaagt
     1081 ccgcggccga tccgcgcagg tacaccatcg cgttgatcga cccgctgccg cccatgccgc
     1141 gacccgagcc catgaacagc gggcggtgca agcacgcggc ctgcggcacc gagaagcgct
     1201 cgtacatcag gctgtcgttg atgaaggcgt ccttgtagcc gtcggcgcgc agggtctccg
     1261 ggtgctgctc ggcggcgtcg ccctgctcga gcaacagcac gcggacgcgc gggtcctgcg
     1321 acagctggcc ggcggcgacg cagccggccg agccgccccc gatgatgacg taatcgaact
     1381 cgaagctgcc tgcgcgggcc tgggtcatgg gcgcgcggac tttatcacga gcgcggcccc
     1441 gggctatgct gccgcgacat ggcggacacc ctgatccggg cgctgatcct cggcgtgcac
     1501 gtgctcggcg
//
LOCUS       DASWNJ010001012         1802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_654593, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001012 DASWNJ010000000
VERSION     DASWNJ010001012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654593"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..1386
                     /locus_tag="VGB85_17100"
     CDS             196..1386
                     /locus_tag="VGB85_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135807.1"
                     /translation="MTDGTGAFAPSSSLRAWTLVIGVVSLAPGCNREPAATQPTAPAT
                     PAASTPPATVAAPVAAPTPATPTPPVEPVVAVTEPVKPLACPPNFPADRPLSERLDLA
                     KQACGALLLVTRETGIAALTSDLVPIAELTKTAGRHLRTHLAGDARQLHYFAADRPDL
                     MRLDLRTGTETVLATLPKIDHACFTDGEPADPVEYIQEDADLGLDVDGGVLCINISDA
                     NVNMMSAGFNYRVDLTTKKVERRMTSVGEECKQPRDREQKPLCQPHEVGPQVTHKSTQ
                     VPGSEELGEPSSSGRYIAYRNTDLGDQGDYIYSAILLYDTTTRTHLAVTSAGPRPVDL
                     AKLKTSADLPPKTCKIPGEASMWWMPDRDVLILENCGDSPFLIVRPEGRTEPLAAQAL
                     AFHE"
     gene            complement(1391..>1802)
                     /locus_tag="VGB85_17105"
     CDS             complement(1391..>1802)
                     /locus_tag="VGB85_17105"
                     /inference="COORDINATES: protein motif:HMM:NF023390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HEY0135808.1"
                     /translation="ASMSVDSTRMSLEDDVLRVRMSGSTRAAEMQQMFDMSEVLFQRH
                     GYILMLIDAKGSKGLDPAARKLHTERLKKFIRPSYTAIYNVNPVARVMSTLIQRGIQL
                     IAGKTFPVSFNKDEAEARAQLDIQRVVLRRAAVE"
BASE COUNT          293 a          701 c          547 g          261 t
ORIGIN      
        1 acgatctccc gcgccgcgct cgcccgcctg tccgagtccg cgcgcgccga cttctcccgc
       61 gccggcgcgt ttcgcctcaa gggcttcgat acccccgagg aggtgtgggt cgaggccgac
      121 gaggccgggc acggcgacgc cgacgaggcc agcgagaccc tcgacctgtg acgggcccgg
      181 ctgtaggctc ccgcgatgac cgacggcacc ggcgcgttcg ctccatcatc atcactgcgc
      241 gcatggacgc tggtcatcgg cgtcgtgtca ctcgccccgg ggtgtaaccg ggagcccgcg
      301 gccacccagc cgaccgcacc cgccacaccc gccgcgagca cacctcctgc caccgtcgcc
      361 gcccccgtcg ccgcccccac accggcgacc cccacgcctc ccgtcgagcc cgtcgtcgcc
      421 gttacagagc cggtgaagcc gctcgcatgc ccgccgaact tccccgcaga tcgcccgctc
      481 agcgagcgcc tcgacctcgc caaacaggcg tgcggcgccc tgttgctcgt cacgcgcgag
      541 acgggcatcg cggcgctcac gagcgacctc gtcccgatcg ccgagctgac gaagaccgcc
      601 ggcaggcacc tgcgcacgca cctcgccggc gacgcgcgcc agctgcacta cttcgccgcg
      661 gaccggccgg acctcatgcg cctcgacctg cgcaccggca cggagaccgt gctcgccaca
      721 ttgccgaaga tcgatcacgc ctgcttcact gacggtgagc ccgccgatcc ggtcgagtac
      781 atccaggagg acgccgacct cggcctcgac gtcgacggcg gcgtgctttg tatcaacatc
      841 agcgacgcca acgtcaacat gatgtcggcc gggttcaact atcgcgtcga cctcaccacc
      901 aagaaggtcg agcgccgcat gacctccgtc ggcgaggagt gcaagcagcc gcgcgaccgc
      961 gagcaaaagc cgctgtgcca gccccacgag gtcggcccgc aggtcaccca caagtcgacc
     1021 caggtccccg gcagcgagga gctcggcgag ccctcctcct cgggccgcta tatcgcgtat
     1081 cgcaacaccg acctcggcga tcagggcgac tacatctatt cggcgatcct gctatatgac
     1141 accacgacgc gcacccacct cgcggtcacg tccgcgggcc cgcgtccggt cgacctggcg
     1201 aagctcaaga cctccgccga cctgcccccg aagacatgca agatcccggg cgaggcgagc
     1261 atgtggtgga tgcccgaccg cgacgtgctg atcctcgaga actgcggcga ttcgccattc
     1321 ctgatcgtcc gccccgaagg ccgcaccgag ccgctcgcgg cccaggcgct cgccttccac
     1381 gaatagccgc tcactcgacc gcggcgcgcc gcaggaccac gcgctggata tccagctgcg
     1441 cgcgcgcctc ggcctcgtcc ttattaaaac tgaccgggaa ggtcttgccg gcgatcagct
     1501 ggatgccccg ctggatcagc gtgctcatca cccgcgccac cgggttgacg ttgtagatcg
     1561 ccgtgtagct cgggcggatg aacttcttga ggcgctcggt gtggagcttg cgcgccgccg
     1621 gatcgaggcc cttcgagcct tttgcgtcga tcagcatcag gatgtacccg tggcgctgga
     1681 acagtacctc gctcatgtcg aacatctgct gcatctcggc ggcccgggtc gacccggaca
     1741 tgcgcacccg cagcacgtca tcctcgaggg acatccgggt cgagtcgacc gacatgctcg
     1801 cg
//
LOCUS       DASWNJ010001013         4016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_583791, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001013 DASWNJ010000000
VERSION     DASWNJ010001013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583791"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VGB85_17110"
     CDS             <1..956
                     /locus_tag="VGB85_17110"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEY0135809.1"
                     /translation="LAAYETTVFLVGEYTEEQSRLQPEFAVADGIVQFFRQEKSNRDE
                     RFMRVLKLRGSRYSEGVHGFRIHETGLQVFPRLVTPEIPVRYQIITERVSTGVEGLDK
                     LLGGGLWRGSTTLLAGPTGAGKTTIGAQFAIEGLRLGEPSLYINFQENPTQLARMFGS
                     FGVDLEEAKGRGLHALYESPVELQIDSVVSRIFRMLNEHNLRRVVIDSLGDLSVAAGD
                     PQRLHDYMYSLTQHFVIHRTTCIITAESSASDFNSSTGIFGLFSYMSDNVVRLEVTIT
                     DRLQRTVCVTKSRSTAHDLHVHTLELGAKGARIIWPSSKGG"
     gene            complement(960..1379)
                     /locus_tag="VGB85_17115"
     CDS             complement(960..1379)
                     /locus_tag="VGB85_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135810.1"
                     /translation="MPRLAPRLSAFLCVLVLASSPGCASRYKQDAEAPTYAAQAKIKV
                     KVNKTGVRSFRVEMIHLAPPARIDPANKGYMVWIAVPGQPTAKAGMLSYSERRRRGVL
                     TATTPHAKFEIIVTIETDSSATVPGPKVILRKIVARS"
     gene            complement(1443..2222)
                     /locus_tag="VGB85_17120"
     CDS             complement(1443..2222)
                     /locus_tag="VGB85_17120"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HEY0135811.1"
                     /translation="MLVAQISDFHVAVPGSQIDRDHQTSAQLERAVAHLGRLRPRPDL
                     VLCTGDLVQDGEGEEYRRLGELLAPLAMPYYLIPGNHDDREALRAAFAGGGYLPAQGL
                     LHYVVDAGPLRLIALDTHVPGAPGGVLCEEQAAWLDAWLTEASERPAIVFMHHPPFRT
                     GIAAMDAMGLADPGALAAVVQRHPQVERVLCGHLHRSIVTRFAGTIASTAPSTGPQLA
                     LDLGPNGRAVFGDEPPAFLLHAWDGQRLVTHLVHVAGEHAS"
     gene            complement(2257..2973)
                     /locus_tag="VGB85_17125"
     CDS             complement(2257..2973)
                     /locus_tag="VGB85_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135812.1"
                     /translation="MTTDHKTDRLLMQMQAASGVLFALFLLAHLGNMMLAALGPAVFD
                     AAQDNLRAVYQVPPIEVALVGAPLLLHIVTSVWRVVRRRRLAQPQPRAVRTRLQRYSA
                     IVLMVFIVGHVVATRGASLLYEVWPGFDAIAFTMLWVPAYFIPYYALFSVAALYHLIN
                     GLSVALPRLGVRRVGPLRDGRVVVGLAAAGGLLLLLGVAGFAGAFSNDVRGRAIDSSY
                     ARLLEELGLADRAAIAGSRE"
     gene            complement(3047..3811)
                     /locus_tag="VGB85_17130"
     CDS             complement(3047..3811)
                     /locus_tag="VGB85_17130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135813.1"
                     /translation="MDILAGSIVMRLVIVASQAPPASPGPVDATAGEPATEPVVPASP
                     EPTVAPSIAEAPAPTPPAPSSAPPPAPADPSNPPDTRPVVRAGPGNWGMTFIFGGLSP
                     ISVAGLGDQQLADGVIFTEIGVRRVFKRVVVPFSIGAGVTSRQNAGDLKPTIAAGVSA
                     SVAVLRGFRVWRRIAPYYGGFVHTHYVDAAGPDNWLVNVSIGPILGIEYYLGDRVSLY
                     GQGIVGIGPDIRKNSVLWGARATLAAGGQLGLTFYF"
BASE COUNT          658 a         1494 c         1306 g          558 t
ORIGIN      
        1 tgctcgcggc ctacgagacc accgtgttcc tggtcggcga atataccgag gagcagtcgc
       61 gcctccagcc cgagttcgcg gtcgccgacg gcatcgtgca gttcttccgc caggagaaga
      121 gcaaccgcga cgagcgcttc atgcgggtcc tcaagctccg cggcagccgc tactccgagg
      181 gcgtgcacgg ctttcgcatc cacgagaccg gcctgcaggt gttcccgcgc ctcgtcaccc
      241 cggagatccc cgtccgctat cagatcatca ccgagcgcgt ctcgaccggc gtcgagggcc
      301 tcgacaagct gctcggcggc ggcctgtggc gcggcagcac caccctgctc gccggcccca
      361 cgggcgccgg caagaccacg atcggcgccc agttcgcgat cgagggcctg cgcctcggcg
      421 agccgagcct ctacatcaac ttccaggaga acccgaccca gctcgcgcgc atgttcggat
      481 cgttcggcgt cgacctcgag gaggccaagg gccgcggcct gcacgccctc tacgagtccc
      541 ccgtcgagct ccagatcgac agcgtcgtca gccgcatctt tcgcatgctc aacgagcaca
      601 acctgcgccg cgtcgtcatc gactcgctcg gcgacctctc ggtcgcggcg ggcgaccccc
      661 agcgcctcca cgactacatg tattcgctga cgcagcactt cgtgatccac cggaccacct
      721 gcatcatcac cgccgaatcg agcgcctccg acttcaactc ctcgaccggc atcttcgggc
      781 tgtttagtta catgtccgac aacgtcgtgc gcctcgaggt caccatcacg gatcgcctgc
      841 agcgcaccgt gtgcgtcacc aagtcgcgca gcaccgcgca cgacctccat gtgcacaccc
      901 tcgagctcgg ggccaagggc gcgcgcatca tctggccgag ctcgaagggc ggctgaccct
      961 cagcttcgcg cgacgatctt gcgcaagatg accttgggac ccggcaccgt cgccgacgag
     1021 tcggtctcga tcgtcacgat gatctcgaac ttcgcgtgcg gcgtggtcgc cgtcagcacg
     1081 ccgcggcggc gccgctcgga gtacgagagc atgcccgcct tggcggtcgg ctgcccgggc
     1141 accgcgatcc acaccatgta gcccttgttg gccgggtcga tcctcgccgg cggcgcgagg
     1201 tggatcatct cgacgcgaaa gctgcgaacc ccggtcttgt tgaccttgac cttgatcttc
     1261 gcctgcgccg cgtaggtcgg cgcctccgcg tcctgcttgt agcgcgacgc gcacccgggg
     1321 ctgctcgcca gcacgagcac acacagaaac gcggacagac ggggggcgag gcgcggcatc
     1381 gcgggcgagg atagcacggc cacgccgacg cccgccgcgc cgcgcagacc cctctgcacg
     1441 cctcagctcg cgtgctcgcc tgccacgtgg accaggtgcg ttaccagccg ctgcccgtcc
     1501 cacgcgtgca gcaggaacgc cggcggctca tcgccgaaga ccgcgcggcc gttcgggccc
     1561 aggtccagcg ccagctgcgg cccggtgctc ggcgccgtgc tggcgatcgt ccccgcgaag
     1621 cgggtcacga tcgaccggtg caggtggcca cacagcacgc gctcgacctg cgggtgccgc
     1681 tgcaccaccg ccgcgagcgc ccctgggtcc gcgagcccca tcgcgtccat cgccgcgatg
     1741 ccggtgcgaa acggcgggtg atgcatgaac acgatcgccg gccgctcgct cgcctcggtc
     1801 agccacgcgt cgagccacgc cgcctgctcc tcgcacaaca ccccgcccgg cgcccccggc
     1861 acgtgggtgt cgagcgcgat cagtcgcaat ggccccgcgt cgaccacgta atgcagcagc
     1921 ccctgcgccg gcagatatcc gcccccggcg aacgccgccc gcagcgcctc gcggtcgtcg
     1981 tgattgcccg ggatcaggta atacggcatc gccagcggtg cgagcaattc gccgagccga
     2041 cggtactcct cgccttctcc gtcctgcacg agatcgccgg tgcacagcac cagatccggc
     2101 cgcggccgca ggcgcccgag atgcgccacc gcgcgctcca gctgggcgct ggtctgatga
     2161 tcccgatcga tctgcgaacc cgggacagcg acgtggaagt cggagatctg ggcgacgagc
     2221 atagcccacg gtatcacacc cgcaggcgac ctcgcctcac tcgcgcgacc ccgcgatcgc
     2281 agcgcggtcg gcgagcccca gctcctcgag cagccgcgca tagctgctgt cgatcgcccg
     2341 cccgcgcacg tcgtttgaaa aggccccggc gaagccggcg acgccgagca gcagcagcag
     2401 gccccccgcc gcggcgagcc ccaccacgac cctgccgtcc cgcagcggcc ccacgcgacg
     2461 cacgccgagc cgcggcagcg cgaccgataa tccattgatc aggtgataca gcgcggcgac
     2521 cgagaacagc gcgtagtacg ggataaaata cgccgggacc cacagcatcg tgaacgcgat
     2581 cgcgtcgaag ccgggccaca cttcataaag cagcgaggcc ccgcgggtcg cgactacgtg
     2641 gccgacgatg aacaccatca atacgatcgc gctgtatcgc tgcaggcgcg tgcgcaccgc
     2701 tcgtggctgc ggctgcgcga gcctccggcg ccgcaccacc cgccacacgc tggtgacgat
     2761 gtgcagcagc agcggcgcgc cgaccagcgc gacctcgatc ggtggcacct gatataccgc
     2821 ccggagattg tcctgcgcgg cgtcgaacac cgccggtccg agcgcggcga gcatcatgtt
     2881 gccgaggtgg gcgagcagga acagggcgaa cagcaccccg ctggccgcct gcatctgcat
     2941 caggaggcga tccgtcttgt ggtcggtggt catgcacccc tgacgatcgc gcctcgcccc
     3001 gcgtgacact tcctgaaatt tcatcgatcc gcgccatttt cgggcgtcag aaatagaacg
     3061 tgagcccgag ctggccgccg gcggccagcg tcgcgcgggc gccccacagc acgctgttct
     3121 tgcggatgtc ggggccgatg ccgacgatcc cctggccata caggctcacg cgatcgccga
     3181 gatagtactc gatgccgagg atcggtccga tgctgacgtt gacgagccag ttgtcgggtc
     3241 cggccgcgtc gacatagtgg gtatggacaa agccgccata atacggggcg atccgccgcc
     3301 acacgcgaaa gccccgcagc accgccaccg acgcgctgac gcccgccgcg atcgtcggct
     3361 tgaggtcgcc ggcgttttgc cggctggtca caccggcgcc gatcgagaac ggcaccacca
     3421 cccgtttaaa aacacggcgc acgccgatct ccgtaaagat cacgccgtcc gcgagttgct
     3481 ggtcgccgag cccggcgacg gagatcggcg agagcccgcc gaagatgaag gtcatgcccc
     3541 agttgcccgg acccgcacgc accaccggcc gggtgtcggg cgggttgctg gggtcggcgg
     3601 gcgcaggcgg cggggcgctc gacggtgcag gaggcgtcgg cgcgggcgcc tctgcgatcg
     3661 acggcgcgac ggtcggctcc ggcgacgcgg gcacgacagg ctcggtcgcc ggctcgcccg
     3721 ccgtcgcgtc gaccggacca ggggacgcgg gaggggcctg cgaggccacg atgacgaggc
     3781 gcatcacgat cgatccagcg aggatatcca tcaccctcga tgctaccgac agtgcagcgc
     3841 gcagtgcagt gacggcagcc gcagagggtc gggcgcgaac gcgcggcggc ggctcatacg
     3901 gggtcggccg tgcccttcga tggtgcgccg cgtcaccgcg gcgccgtcaa tcctccgacg
     3961 atcgctgcat cacgagcacc ccgtcgcgca gaccgacgag atcgaggatg ccatcg
//
LOCUS       DASWNJ010001014         4107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_195849, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001014 DASWNJ010000000
VERSION     DASWNJ010001014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195849"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..1104)
                     /locus_tag="VGB85_17135"
     CDS             complement(157..1104)
                     /locus_tag="VGB85_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135814.1"
                     /translation="MPASKKWAAIIGGLLVLVGCGASPGNDEGTSGTGSEGSTGTGTV
                     PTGTGDGGESSTGGGTGGLDACDPELPAIAEEEFAERFAAAICEQKAACGCAPEFACE
                     TYFVDELAAVREQAQAAGLAYDGVCAARKLVGLVEQHGCEMASQVEIGPTCAFECAVY
                     RGDIPPGNECPVLPMPNGFLMSRFIEDCAAPGTCDYYYELVCTSPLPVVGPGEACRDQ
                     NAEEIATCEPGHSCDFAQRECVPSLGPGDPCAEQSCPYPLYCSEAELCTAPGETGASC
                     TQKGECLSQRCGDAGCEDWVWICEVAEIVDLFTRNPEVI"
     gene            1138..2007
                     /locus_tag="VGB85_17140"
     CDS             1138..2007
                     /locus_tag="VGB85_17140"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0135815.1"
                     /translation="MAGPSTFLRDATLIAGFELRESLRTRRALVLVLLYVLIASAASY
                     IYVRIVATIDNVTALAGGQDAALAAAQDQGYHEMLLFFAGGDATLANHLATYPAMVLI
                     FAWVSLAFLPWFIALTSYDQIAGELHLRTIRYAALRTSRGAYVLGKLLGQAVLVAGVA
                     ALGMLPVVLIGAIWLPNFAALATITALLSTWPVTVVCALAFLGVVSLASQLTRTPGTA
                     RAAAVGLLFGAWLLSLLTNTYPWLAVLSPWYWKPGLYQPGIADRLVAAAGCLGLCAGY
                     TALGFLRFRRRDL"
     gene            2022..2954
                     /locus_tag="VGB85_17145"
     CDS             2022..2954
                     /locus_tag="VGB85_17145"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0135816.1"
                     /translation="MTELALHLDRVHKRFGNVHALRGADLKVPRGTIFALIGPNGAGK
                     TTLFGLCCGYLRADQGKVEILGGPPDPSHLKGRLGALPQDALLGREITVREHLVFLAR
                     LQGMDPATAGREATRVLGLVDLAELAERRAGALSHGQVKRVGVAQAFLGSPDLILLDE
                     PTAGLDPRHAHDLRALIRSQRGAQTIVVSSHNLQELEAICDEAAFMEKGVTIESGKVA
                     ALTAQDAEFFVQLAPGPEPLEALRARVEATSITWEPERRTLRVCFHPTPTRVAEDVIA
                     EVLRELLAAGARVSALGKGRSLEQRYLEQAAATS"
     gene            complement(2951..>4107)
                     /locus_tag="VGB85_17150"
     CDS             complement(2951..>4107)
                     /locus_tag="VGB85_17150"
                     /inference="COORDINATES: protein motif:HMM:NF040941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0135817.1"
                     /translation="NSCALASCGDGEVQAGEACDDGNQSLTDDCLSTCVLASCGDGAL
                     HAGVELCDDGNPDDADACLNTCVPAKCGDAVVQAGVEECDDANAEETDACLNNCVVAK
                     CGDAVVQAGVEECDDANAEDTDACLGTCKAAKCGDQVLQAGVEQCDDGNLDNTDGCTT
                     TCTVPKNCLEVQMAFPGATDGVYLVDPDGAEGMPGFQAFCDMTTAGGGWTVVERSPFG
                     GLTIGKALFNDLPVNVDDPKASRYRLAKPMMTALRDVSTDMRLDCRGQDFLLTAASNL
                     FNGQGGPAGCNNWTKVLYKEAQLKGNKVMNKTICTWNLGASEGCAGAWHIDETAQNQY
                     GCGLTNYPWKGVAVVSPSADTFATDAVTLDGTNPVHDCHKNSAVRWVMVR"
BASE COUNT          555 a         1649 c         1248 g          655 t
ORIGIN      
        1 gagggcctgc catggagtcg ccgccgcgaa caggagcgcg tcgacgcccg ggatcgccga
       61 ggccgggaac ggcagcatgt agagcacgag gtaggcgacg acccagaggc gaagtatctg
      121 caggaccgac acgcctgcga gcataccacc gcgcggctag atcacctccg ggttgcgcgt
      181 gaacagatcg acgatctccg cgacctcgca gatccacacc cagtcctcgc agcccgcgtc
      241 cccgcagcgc tgcgagaggc actcgccctt ctgcgtgcag ctcgcccccg tctctccggg
      301 cgccgtgcac agctcggcct ccgagcagta cagcggatac gggcagctct gctccgcgca
      361 ggggtccccc gggccgaggc tcggcacaca ctcgcgctgc gcgaagtcgc aggaatgacc
      421 cggctcgcag gtcgctatct cctccgcgtt ctggtccctg cacgcctctc cggggccgac
      481 caccggcaac ggcgatgtgc acaccagctc gtaataatag tcgcaggtgc ccggcgccgc
      541 gcaatcctcg ataaagcgcg acatcaggaa accgttcggc atcggcagga ccgggcactc
      601 gttcccggga gggatgtctc cgcgatacac cgcgcactcg aacgcgcagg tcggcccgat
      661 ctcgacctgc gacgccatct cgcagccgtg ctgttcgacc aggccgacca gcttgcgcgc
      721 cgcgcatacg ccgtcgtagg ccagcccggc cgcctgcgct tgctcgcgga ccgccgccaa
      781 ttcatcgacg aagtacgtct cgcaagcaaa ttccggcgcg cagccgcacg cggccttttg
      841 ctcgcagatc gccgccgcga agcgctcggc gaattcctcc tcggcgatcg ccggcagttc
      901 cgggtcgcag gcgtcgagcc ccccggtccc gcccccggtc gacgactcgc cgccatcgcc
      961 cgtcccggtc ggcaccgtgc ccgtccccgt cgagccctcg ctgcccgtcc cggacgtccc
     1021 ctcgtcattg cccggtgacg cgccgcatcc cacgagcacg agcaaccccc cgatgatcgc
     1081 tgcccacttt ttgctggccg gcataccgcg accgtatcac gtgctacagt ccgcgccgtg
     1141 gccggcccca gcacctttct gcgcgacgcg acgctgatcg caggcttcga gctgcgcgag
     1201 tcgctgcgca cgcgccgggc cctcgtgctc gtgctgttgt acgtcctgat cgccagcgcc
     1261 gcgagctaca tctacgtccg gatcgtcgcc acgatcgaca acgtcaccgc gctcgccggt
     1321 ggtcaggacg cggcgctcgc cgccgcgcag gatcagggct atcacgagat gctgctgttc
     1381 ttcgccggcg gcgacgcgac gctggccaac cacctcgcca cctatccggc gatggtcctg
     1441 atcttcgcct gggtctcgct cgcgtttctc ccctggttca tcgccctgac cagctacgac
     1501 cagatcgccg gcgagctgca cctgcggacg atccgctacg ccgccttacg gacctcgcgc
     1561 ggcgcctacg tgctcggcaa gctgctcggc caggccgtgc tcgtcgccgg cgtcgccgcg
     1621 ctcggcatgc tccccgtcgt gctgatcggc gcgatctggc tgcccaactt cgccgcgctc
     1681 gccacgatca ccgccctgct gtcgacctgg ccggtcaccg tcgtgtgcgc gctcgccttc
     1741 ctcggcgtcg tctcgctcgc ctcgcagctg acccgcaccc ccggcaccgc gcgggccgcc
     1801 gccgtcggcc tgctgttcgg cgcgtggctg ctgtcgctgc tgaccaacac ctatccctgg
     1861 ctcgccgtgc tctccccctg gtactggaag cccggcctgt atcagccggg catcgccgac
     1921 cgcctcgtcg ccgccgccgg ctgcctcggc ctgtgcgccg gttacaccgc gctcggcttc
     1981 ctccgctttc gtcgccgcga cctgtgacca gggacctcct catgaccgag ctcgccctcc
     2041 acctcgatcg cgtccacaag cgcttcggca acgtgcacgc gctgcgcggc gcggacctca
     2101 aggtcccgcg cggcacgatc ttcgcgctga tcggccccaa cggcgccggc aagaccaccc
     2161 tcttcggcct gtgctgcggc tacctgcgcg ccgatcaggg caaggtcgag atcctcggcg
     2221 gcccgcccga cccgagccac ctcaagggtc gcctcggcgc gctcccgcag gacgccctgc
     2281 tcggccgcga gatcaccgtg cgcgagcacc tggtgttcct cgcccggctg cagggcatgg
     2341 accccgcgac cgccgggcgc gaggcgaccc gcgtgctcgg cctcgtcgac ctcgccgagc
     2401 tcgccgagcg ccgcgctggc gccctctcgc acggtcaggt caagcgcgtc ggcgtcgccc
     2461 aggccttcct cggcagcccc gacctcatcc tgctcgacga gccgaccgcc ggcctcgacc
     2521 cgcgccacgc ccacgacctg cgcgcgctga tccgcagcca gcgcggcgcg cagacgatcg
     2581 tcgtctcctc gcacaacttg caggagctcg aggcgatctg cgacgaggcc gccttcatgg
     2641 agaagggcgt cacgatcgag tccggcaagg tcgcggccct gaccgcgcag gacgccgaat
     2701 tcttcgtcca gctcgccccc ggccccgagc cgctcgaggc cctgcgcgcc cgcgtcgagg
     2761 cgacgagcat cacctgggag cccgagcgcc ggaccctgcg cgtctgcttc cacccgaccc
     2821 ccacacgcgt cgccgaggac gtgatcgccg aggtcctgcg cgagctgctc gcggcgggcg
     2881 cacgcgtcag cgcgctcggc aagggccgct cgctcgagca acgttacctc gagcaagcgg
     2941 ccgccacttc ctaacgcacc atcacccagc gcaccgccga gttcttgtgg cagtcgtgca
     3001 ccgggttggt gccgtcgagc gtcaccgcgt cggtggcgaa ggtgtcggcc gacgggctga
     3061 ccaccgcgac gcccttccag gggtagttcg tcaggccgca gccgtactga ttctgcgccg
     3121 tctcgtcgat gtgccacgcg cccgcgcagc cctccgacgc cccgaggttc caggtgcaga
     3181 tcgtcttgtt catcaccttg ttgcccttga gctgcgcctc cttgtacagc accttggtcc
     3241 agttgttaca acccgccggc ccgccttgcc cgttgaacag gttgctcgcc gccgtcagca
     3301 ggaagtcctg tccgcggcaa tcgaggcgca tgtcggtcga gacgtcgcgc agcgccgtca
     3361 tcatcggctt ggccaggcga tagcgcgacg ccttcggatc gtcgacgttg accggcaggt
     3421 cattgaacag cgccttgccg atcgtcagcc cgccgaaggg gctgcgctcg accaccgtcc
     3481 agccgccgcc cgccgtcgtc atgtcgcaga acgcctggaa gcccggcatg ccctccgcgc
     3541 cgtccgggtc gacgagatac accccgtcgg tcgcccccgg gaacgccatc tgcacctcga
     3601 gacaattctt gggcaccgtg cacgtcgtcg tgcaaccatc ggtgttgtcg aggttgccgt
     3661 cgtcgcactg ctccactccg gcctgcagca cctggtcgcc gcacttcgcc gccttgcagg
     3721 tccccagaca cgcatccgtg tcctccgcgt tggcgtcgtc gcactcctcc accccggcct
     3781 gcaccaccgc gtcgccgcac ttggcgacca cgcaattgtt gaggcacgcg tccgtctcct
     3841 ccgcgttggc gtcgtcgcac tcctccaccc ccgcctgcac caccgcgtcg ccgcacttcg
     3901 ccggcacgca agtattgagg cacgcgtccg cgtcgtccgg gttgccgtcg tcacacagct
     3961 cgaccccggc gtgcagcgca ccgtcaccgc agctcgccag cacacacgtg ctcaggcagt
     4021 cgtccgtcag gctctggttg ccgtcgtcgc acgcctcgcc cgcctgcacc tccccgtcgc
     4081 cacagctcgc aagcgcgcag ctgttgc
//
LOCUS       DASWNJ010001015         5841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_54097, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001015 DASWNJ010000000
VERSION     DASWNJ010001015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54097"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="VGB85_17155"
     CDS             <1..539
                     /locus_tag="VGB85_17155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135818.1"
                     /translation="EEAPVVRAGEVPAAAPRGLPRRGRLLAGERVARVPRWPAAIDQA
                     TRERLPAPARAAVERSPVPVLVPADPGWLSSTQVFPGSWGYALAARDGEVTLALHASR
                     IATIVPGVGVATGTHRVRGVDGFLSVNEGIRTASWIEHGVAYSLDLECRDPQACSEAA
                     LRAAVEGLVYVGGVGGGA"
     gene            536..2227
                     /locus_tag="VGB85_17160"
     CDS             536..2227
                     /locus_tag="VGB85_17160"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY0135819.1"
                     /translation="MIRRIVVLAALLVPGTAAAEPFYLPPGDLLPDSGQGIAEYTVFA
                     PGMRFPLAAGPAYPNSQVYMNGGSHGPGGNQCDAINYVYPWRDNYCETRSWEMPLCPA
                     GEGHQGQDIRGPTCEKDKHQIVATAAGEVTNIGSYSIYVTTPAGQRFDYLHGSLDTIV
                     VSMNQQVATGAPLVKLSNNMGDTPTSIHLHFNIKQDVGGVGNVYVSPYMSLVRAYEEL
                     MGLGNKPPTGSFDDSGCTAITGWAQDPDAPDAPVLVDLYFGGPKGDMNAVPVQVTADL
                     PREDLCMALGSCDHGFELEPPLSLRDGQAHAVHAYALDSESGVQAELPLSPAQLQCEP
                     PALPPGQRRWVTDPESMVAWKFSPFWQAIHLAQAEVDAVPQDRDLGAAPLLAVSDEDP
                     ATVWLVDDKFRRLVPGPEVAAAWGFDLAAASTWPAADLAALIAGTPVRPQPVLLQGAG
                     PELYVLDDHQCDPAAQPVDPLCSESGTQTAGADDTGADTGHEGGQLSAGQGDTSDAQD
                     TGASDAQGGSDVDALPPGYGQDGDGACGCTQPGPSAPVLALVGALGLLGRRRRRR"
     gene            2334..5246
                     /locus_tag="VGB85_17165"
     CDS             2334..5246
                     /locus_tag="VGB85_17165"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0135820.1"
                     /translation="MSVPPSSSSQLLTRAETVATEPTRSAEAEAAGPNRIGRFVVLRK
                     LGEGGMGIVYAAYDEELERKVAIKLLRADFARHQQSIGQARLLREAQAMARLSHPNVV
                     QIYEVGKFGDAVFIAMEFVSGCDLREYLRRSERPWRKILGVYMQAGHGLAAAHAAGII
                     HRDFKPDNVLVGDDGRVRVDDFGLARPDEATLEAAASAGTSGVFAHGASGSYANVELT
                     QAGTFIGTPAYMSPEQLQRNPADALSDQFSFCVALFEGLHGYRPFAGADAGQLTQAVL
                     FGKPLDPPRDSAVPGWVHQVLLRGLARAPEQRHKGMDALLQALAADPAITRRRRLGVA
                     GLVLAAGLAGGGGYALHELGAQQNGSCGGGEQRLAAVWDEATAGRVRAALAGAASGYA
                     GDVADRVESRLGAYATAWTAMHRDACEVHQRGEQSDALYDLRMACLDDRKTALRALVG
                     VLASPDAAVVEKAVQAAEHLPLLGRCADASALLAQVPPPEDPAVAQAAQALAVRLAEA
                     RARLEVGQFQPALQDTASLRGEAEALGYAPTLAAALHLEGLIADQLGDYATSEAALLR
                     AVTTADAAGDDDERAAATIDLARAIGLRQARFAEGLHVAALARGSVARLADRSTAEAT
                     LETRVGEILLQRGALDEASAHIERSVDLHAQLYGETSTAYAAAVNARATLRFFRADYA
                     EALAEYRRVAEIYERAYGPAHPIMGKVLNNIGAAELSLFDYAAAQQTYTRALVVLRAA
                     HGASHPSLATVYSNLGLIAFAQAEFPRAIEEFRRSLAIYEVSLPANHPSIGDCLQDLG
                     NGQLIAGQYAEAEATFERALAVYLAAYGPGHEQTTQALAHLGMAQLRGGHRAAAARSF
                     QRALTEFRGDPKDPALGRPRAGVGALRLAEGDFKAAVTELDEALRLLGEGKAPPQELA
                     EVQFALARALWAADRTNAERARTLAAGARTGFLGFGAAYRDTAAEVETWLAGLPPA"
     gene            complement(5254..5568)
                     /locus_tag="VGB85_17170"
     CDS             complement(5254..5568)
                     /locus_tag="VGB85_17170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135821.1"
                     /translation="MIIGYGGGGGGGGGTTTNAAALKGEEPCILDKEIECECFKGVGL
                     IEACIKSGVTLDCINPETPYAELTQKGLVLIAGKEPYLGNLLCSGESDDTAYVTLALP
                     KG"
     gene            5588..>5841
                     /locus_tag="VGB85_17175"
     CDS             5588..>5841
                     /locus_tag="VGB85_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135822.1"
                     /translation="MATAELLATTPTVTNAAMVKNFRMTIPPDGPDPTILDKEAPRGR
                     QPANAGEHGDKALRKRCHIWMAARQTCTDNAYIATDPGHT"
BASE COUNT          807 a         2050 c         2213 g          771 t
ORIGIN      
        1 gcgaggaggc gccggtcgtg cgggcggggg aggtccccgc cgcggcgccg cggggcctgc
       61 cgcgacgcgg acggctgctc gccggggagc gcgtggcccg ggtgccgcgc tggcccgcgg
      121 cgatcgatca ggcgacgcgc gagcggctgc cggcgccggc ccgcgcggcg gtcgagcgct
      181 cgccggtgcc cgtgctcgta ccggcggatc cagggtggct gagctcgacg caggtgttcc
      241 ccgggagctg ggggtatgca ctggcggcgc gcgacgggga ggtgaccctc gcgctgcacg
      301 cctcgcggat cgccacgatc gtgccaggcg tcggcgtcgc gacgggcacg caccgcgtgc
      361 gtggcgtcga cggcttcctc agcgtcaacg aggggatccg tacggcctcg tggatcgagc
      421 atggtgtcgc ttactcgctc gatctcgagt gtcgagatcc gcaggcgtgc agcgaggcgg
      481 cgctgcgggc cgcggtcgag ggcctcgtgt atgtcggcgg cgtcggaggt ggcgcgtgat
      541 ccgtcgtatc gtcgtgctgg cggcgttgct ggtcccgggt acggcggcgg ccgagccgtt
      601 ttatctgccg ccgggggacc tgctgccgga ctccggccaa gggatcgcgg agtacaccgt
      661 gttcgcgccg ggcatgcgct tcccgctcgc ggccgggccg gcgtacccga actcgcaggt
      721 gtacatgaac ggcggctcgc acgggcccgg cgggaaccag tgcgacgcga tcaactacgt
      781 gtatccgtgg cgcgacaact actgcgagac gcgcagctgg gagatgccgc tgtgcccggc
      841 cggcgagggg caccagggcc aggacatccg cggcccgacc tgcgagaagg acaagcacca
      901 gatcgtcgcc acggcggcgg gcgaggtcac caacatcggg tcgtactcga tctacgtgac
      961 gacgccggcg ggccagcgct tcgattatct gcacggctcg ctcgacacga tcgtggtcag
     1021 catgaaccag caggtcgcga ccggggcgcc gctggtcaag ctgtcgaaca acatgggcga
     1081 cacgccgacg tcgatccatc tccacttcaa catcaagcag gacgtcggcg gggtcgggaa
     1141 cgtctatgtg tcgccgtaca tgtcgctggt gcgcgcgtac gaggagctga tgggcctcgg
     1201 caacaagccg ccgaccggga gcttcgacga cagcggctgc acggcgatca ccggctgggc
     1261 gcaggacccc gacgcccccg acgcgccggt gctcgtcgac ctgtacttcg gcgggcccaa
     1321 gggcgacatg aacgcggtgc cggtgcaggt gaccgccgac ctgccgcgcg aggacctgtg
     1381 catggcgctc gggtcgtgcg accacggctt cgagctcgag ccgccgctga gcctgcgcga
     1441 cgggcaagca catgcggtgc acgcctacgc gctcgacagc gagagcggcg tgcaggccga
     1501 gctgccgctg tcgcccgcgc agctgcagtg cgagccgccc gcgttgccgc cgggccagcg
     1561 ccgctgggtg accgacccgg agagcatggt cgcgtggaag ttctcgccgt tttggcaggc
     1621 gatccacctc gcgcaggcgg aggtcgacgc ggtgccgcag gaccgcgacc tcggggccgc
     1681 gccgctgctg gccgtcagcg acgaggatcc ggcgacggtg tggctggtcg acgacaagtt
     1741 ccgccgcctg gtgccggggc ccgaggtcgc cgcggcgtgg ggcttcgacc tcgcggcggc
     1801 gagcacctgg ccggccgccg atctcgcggc gctgatcgcc ggcacgccgg tgcggccgca
     1861 gccggtcctg ctgcaaggcg caggtcccga gctgtacgtg ctcgacgatc accagtgcga
     1921 tcccgccgcg cagccggtgg atccactgtg tagtgaaagc ggcacgcaga ccgcgggcgc
     1981 agacgacacc ggggccgaca ccggacacga gggcggccag ctcagcgcgg gtcagggcga
     2041 tacgagcgac gcccaggaca ccggcgcgag cgatgcccag gggggaagcg acgtcgatgc
     2101 gctgccgccc ggctacggtc aggacggcga cggcgcctgc ggctgtaccc agcccggacc
     2161 ctccgcgccg gtgctggcgc tggtcggcgc gctcggcctg ctcggccgac gccgacgccg
     2221 acgctgaccg cgaaggggcg gtctcgcccc gcgcggcgtg taggaagcgc ttgcgtcggg
     2281 ggccgcctcg ccgggaagat cgtcccggtg tcccgcacga ggagggcaca cggatgtctg
     2341 tcccgccttc gtcctccagc cagttattga cgcgggccga gacggtggcg acggagccga
     2401 cgcgcagcgc ggaggccgag gccgcgggcc cgaatcgcat cggccgcttc gtggtcctgc
     2461 gcaagctcgg cgagggcggc atgggcatcg tgtacgcggc ctatgatgaa gagctcgagc
     2521 gcaaggtcgc gatcaagctg ctgcgcgccg acttcgcccg ccaccagcag agcatcggcc
     2581 aggcccggct gttgcgcgag gcgcaggcga tggcccgcct gtcgcacccg aacgtcgtgc
     2641 agatctacga ggtcggcaag ttcggcgacg cggtgttcat cgccatggag ttcgtcagcg
     2701 gctgtgacct gcgcgagtac ctgcgccgca gcgagcgacc gtggcgcaag atcctcggcg
     2761 tgtacatgca ggccgggcac ggcctggcgg cggcccacgc cgcggggatc attcaccgcg
     2821 acttcaagcc ggacaacgtg ctagtcggcg acgacgggcg cgtgcgcgtc gacgacttcg
     2881 ggctcgcgcg ccccgacgag gcgacgctcg aggcggccgc gagcgccggg acctccgggg
     2941 tgttcgcgca cggggcctcg gggtcgtacg cgaacgtcga gctgacgcag gccgggacct
     3001 tcatcggcac gccggcgtac atgtcgccgg agcagctgca acgcaacccg gccgacgcgc
     3061 tgagcgatca gttcagcttc tgtgtcgcgc tgttcgaggg gctgcacggc tatcgcccgt
     3121 tcgccggcgc cgacgccggc cagctgacgc aggcggtgct gttcggcaag ccgctcgacc
     3181 cgccgcgcga cagcgccgtg ccgggctggg tgcaccaggt gctgctgcgc gggctggcgc
     3241 gcgcgccgga gcagcgccac aagggcatgg acgcgctgct gcaggcgctc gcggccgacc
     3301 ccgcgatcac gcggcggcgg cggctcggcg tcgccgggct ggtgctcgcg gccgggctcg
     3361 cgggcggcgg cgggtatgcg ctgcacgagc tcggcgcgca gcaaaacggc agctgcggcg
     3421 gcggtgaaca gcggctcgcg gcggtgtggg acgaggcgac cgccgggcgc gtgcgggcgg
     3481 ccctcgccgg ggccgcgagc ggctacgccg gcgatgtcgc cgaccgcgtc gagagccgcc
     3541 tcggtgccta tgcgacggcg tggacggcga tgcaccgcga cgcgtgtgag gtgcaccagc
     3601 gcggcgagca gtcggacgcg ctctacgacc tgcgcatggc ctgcctcgac gaccgcaaga
     3661 cggcgctgcg ggccctcgtc ggcgtgctgg cgagcccgga cgcggcggtc gtcgagaagg
     3721 cggtgcaggc ggccgagcac ctgccgctgc tcggccgctg cgccgacgcg agcgcgctgc
     3781 tggcccaggt cccgccgccc gaggaccccg cggtggcgca ggcggcccag gccctcgcgg
     3841 tgcgcctggc cgaggcgcgc gcgcggctcg aggtcggtca gttccagccg gccttgcagg
     3901 acaccgcgag cctgcgcggc gaggccgagg cgctcggcta cgcgccgacg ctggcggccg
     3961 cgctgcatct cgagggcctg atcgcggatc agctcggcga ctacgcgacc tcggaggcgg
     4021 cgctgctgcg cgcggtgacg acggccgacg cggccggcga cgacgacgag cgggccgcgg
     4081 cgacgatcga cctggcgcgc gcgatcggcc tgcgacaggc ccgcttcgcg gagggcctgc
     4141 acgtcgcggc gctggcccgc ggcagcgtcg cgcggctcgc cgatcgcagc accgccgagg
     4201 cgacgctcga gacgcgggtc ggcgagatct tgctgcagcg cggcgccctc gacgaggcga
     4261 gcgcgcacat cgaacgttcg gtcgatctgc acgcgcagct gtacggcgag acctcgaccg
     4321 cgtacgcggc ggccgtcaat gcgcgcgcga ccctgcggtt ctttcgcgcc gactatgccg
     4381 aggcgctggc ggagtaccgc cgggtcgccg agatctacga gcgggcctac ggcccggcgc
     4441 acccgatcat gggcaaggtg ctcaacaaca tcggcgccgc cgagctgagc ctgttcgact
     4501 acgcggcggc ccagcagacg tacacgcgcg cgctcgtggt cctgcgcgcg gcccatggcg
     4561 cgagccatcc gtcgctggcg acggtgtact cgaacctcgg gctgatcgcg ttcgcccagg
     4621 ccgagttccc gcgcgcgatc gaggagtttc gccggtcgct ggcgatctac gaggtgtcgc
     4681 tgccggcgaa ccacccgagc atcggcgact gcctgcaaga cctcggcaac gggcagctga
     4741 tcgccggcca gtacgccgag gcggaggcga ccttcgagcg cgcgctcgcg gtgtacctcg
     4801 ccgcctacgg gccgggccac gagcagacga cgcaggcgct ggcccacctc ggcatggcgc
     4861 agctgcgcgg cggacatcgg gccgcggcgg cccgcagctt ccagcgcgcg ctgaccgagt
     4921 ttcgcggcga cccgaaggac cccgcgctcg gccgtccgcg cgccggtgtc ggcgcgctgc
     4981 gcctggcgga gggcgacttc aaggcggcgg tgacggagct cgacgaggcg ctgaggttgc
     5041 tgggcgaggg caaggcgccg ccgcaggagc tggccgaggt gcagttcgcg ctggcacgcg
     5101 cgctgtgggc ggccgaccgg acgaacgccg aacgggcgcg gaccctggcg gccggcgccc
     5161 gtactggttt cctcggcttc ggcgccgcgt atcgtgacac cgcggccgag gtggagacct
     5221 ggctcgccgg cctgccgcca gcgtgacccg tttttagccc ttgggcagcg ccagggtcac
     5281 gtaggcggtg tcgtcggact cgccgctgca cagcaggttg ccgaggtacg gctccttgcc
     5341 ggcgatcagc acgaggccct tttgggtcag ctcggcgtag ggcgtctcgg ggttgatgca
     5401 gtcgagcgtc acgccgctct tgatgcacgc ctcgatcagg ccgacgccct tgaagcactc
     5461 acactcgatc tccttgtcga ggatgcacgg ctcttcgccc ttgagcgcgg ccgcattggt
     5521 ggtcgtgccg ccaccgccac cgccaccgcc gccgtaaccg atgatcacgc gacccggata
     5581 catgtaattg gcgaccgccg agctgctggc gaccacaccc actgtcacaa acgccgcgat
     5641 ggtgaaaaac tttcgcatga caattccccc cgatgggccc gatcccacga tccttgataa
     5701 agaagcgccc cggggccgtc aaccggcaaa tgccggcgaa catggggata aggccctgcg
     5761 caagcgctgt cacatatgga tggccgcgcg tcagacgtgc accgacaatg cgtacatcgc
     5821 cacggacccc ggccatactt g
//
LOCUS       DASWNJ010001016         1618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_370581, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001016 DASWNJ010000000
VERSION     DASWNJ010001016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370581"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="VGB85_17180"
     CDS             <1..1018
                     /locus_tag="VGB85_17180"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0135823.1"
                     /translation="DFDGDGRLDLALATHSAGGHAVTFAINDGQGGLRPTTGLKFPMT
                     PTAIQAADLDRDGSLDLAIAAVPAAGSGDDPAVHVLLGRGAGQFVAGAVATRVRPVGL
                     WISDFTGDGLTDILVLDADGRTVEVLRATGNGELEAGPRSRLPGKVGPERLTLGDFDL
                     DRRLDLALMYDRGGKSEAIVAIARGDGHGEFKLAARRVIGRRGRALVAADLNRDGLAD
                     LTALAESAGAGPIAAVLLGDGALGFSAISYFGPDAVADAIVADIDRSAGPDLLVSSQR
                     GDAVRIVPGDARGGFGPVITVAAGAAGQVARVADLDGDGRPELLSHGPQAPGLTVLRP
                     RPCR"
     gene            complement(1032..>1618)
                     /locus_tag="VGB85_17185"
     CDS             complement(1032..>1618)
                     /locus_tag="VGB85_17185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0135824.1"
                     /translation="RDRAQGGLGIGLTLARRLVELHGGRIVADSAGPGQGCRMTVHLP
                     LAPQAVPDLAAEAPPDTPALDDNGQRIVIVEDNDDVREMLRYLLESIGHEVDEAADGV
                     QGIAKVAELQPDVALVDIGLPGLDGFEVARRLRASGAVSGTLLIALTGYGLEKDREAV
                     LEAGFDRHLTKPVEPDLLIQILQEHREARRKRKL"
BASE COUNT          187 a          644 c          578 g          209 t
ORIGIN      
        1 cgacttcgac ggcgacggcc gcctcgacct cgcgctggcg acccacagcg ccggcgggca
       61 cgcggtgacc ttcgcgatca acgacggaca gggcgggctg aggccgacca ccggcctcaa
      121 gttcccgatg acgccgaccg cgatccaggc cgccgacctc gaccgcgacg gctccctcga
      181 cctcgcgatc gcggccgtgc ccgccgcggg ctccggggac gaccccgcgg tgcacgtgct
      241 gctcggtcgc ggcgccggtc agttcgtcgc cggggccgtg gcgacgcgcg tgcgccccgt
      301 cggcctgtgg atctccgact tcaccggcga cggcctgacc gacatcctcg tgctcgacgc
      361 cgacggtcgc accgtcgagg tcctgcgcgc caccggcaac ggcgagctcg aggccgggcc
      421 gcgctcgcgg ctgccgggca aggtcggccc ggagcgcctc accctcggcg acttcgacct
      481 cgacaggcgc ctcgacctcg ccctgatgta cgaccgcggc ggcaagtccg aggcgatcgt
      541 ggcgatcgcc cgcggcgacg gccacggcga gttcaagctc gccgcgcgcc gcgtgatcgg
      601 tcgtcgcggt cgggcgctcg tcgccgccga cctcaaccgt gatggcctcg ccgacctcac
      661 cgcgctggcc gagagcgccg gcgccggacc gatcgccgcc gtgctgctcg gcgacggcgc
      721 gctcggcttc tccgcgatca gctacttcgg ccccgacgcg gtcgccgacg cgatcgtcgc
      781 cgacatcgac cgcagcgccg ggcccgacct gctcgtcagc tcgcagcgcg gcgacgccgt
      841 gcggatcgtg cccggggatg ctcgcggcgg cttcggtccg gtcatcacgg tcgccgccgg
      901 cgcggccggc caggtcgcca gggtcgccga cctcgacggc gacggccgcc ccgagctgct
      961 cagccacggc ccgcaggcgc ccggcctgac cgtcctgcgc ccacggccgt gccgctaaag
     1021 cgcccgcgtg ctcacagctt gcgcttgcgc cgggcctcgc ggtgctcctg caggatctgg
     1081 atcagcaggt cgggctcgac cggcttggtg aggtgacgat cgaagccggc ctcgagcacc
     1141 gcctcgcgat ccttctccag cccatacccg gtcagcgcga tcagcagggt ccccgacacc
     1201 gcgccgctcg ctcgcagccg ccgcgcgacc tcgaagccgt cgaggcccgg caggccgatg
     1261 tcgaccagcg cgacgtccgg ctgcagctcg gcgaccttgg cgatcccctg gacgccgtcg
     1321 gcggcctcgt cgacctcgtg gccgatgctc tcgagcaggt agcgcagcat ctcgcggacg
     1381 tcgtcgttgt cctcgacgat gacgatgcgc tggccgttgt cgtcgagcgc gggggtgtcc
     1441 gggggcgcct cggcggcgag gtcggggacc gcctgcggcg cgagcggcag gtgcaccgtc
     1501 atgcgacagc cctgccccgg cccggcgctg tcggccacga tccggccgcc gtgcagctcg
     1561 acgagccgac gcgcgagcgt caggccgatg ccgaggccgc cctgggcccg gtcgcgcg
//
LOCUS       DASWNJ010001017         2196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_266683, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001017 DASWNJ010000000
VERSION     DASWNJ010001017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266683"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="VGB85_17190"
     CDS             <1..994
                     /locus_tag="VGB85_17190"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY0135825.1"
                     /translation="VATLIASLAMHLLLWPIGDRLIKLSWSDPPPPPGEGFMQVALVD
                     EDKPPDPDDEVPRETDPPGKLIKQDRVKKEAVPDDAKHVAEFDQKVEDETRAARGRAK
                     PGAAPTTPGTSPDANNLPATPNLLDPRPAEPKQASGSPGEADEGRGDPATPKVPSPRS
                     LMPLLPEMGPSGRPGVRGNPSEMSPADPGQHGSMDDIQDVEEGDVNQLNSRRWKYASF
                     FNRVRDQVAQHWHPEVVHAARDPDGSRYGIKTRVTRLLIRLNPDGSLNKIKIDQHSGV
                     DFLDEEAIRAVRAAHPFANPPAQLVDAETGFIDFGFGFIFEIESGKPQIFRYRR"
     gene            1044..2111
                     /locus_tag="VGB85_17195"
     CDS             1044..2111
                     /locus_tag="VGB85_17195"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY0135826.1"
                     /translation="MTLIKRLFVVVLLAVALALAYLALAPSAVDPVQWNPPPVPEMAG
                     PLAPNAHLALAELIAQGHVDGPEDVETDAQGRLYAGTNAGTVVRVENGKVEVFATTGG
                     RPLGLDFAPDGQLIVADAIKGLLAISPDGKQITTLADAADGVAFGFTDDVAVARDGTI
                     YFSDASDKFGFGDHMYDLLEGRPHGRLMQYDPTSKTTKVLLTDLYFANGVALAGDESF
                     VIVSETYRYRLTRYWLKGPKAGSTDVLAEGLPGFPDGVSQSGRGTFWVAIFTVRNKAG
                     DFLAPRPLLKRMVANLPRFLWPKPDPYGLIIELDEQGNVLRSLHDASGAYVEQVTSVH
                     ERDGKLYLGHLHRDRITRVGL"
BASE COUNT          356 a          813 c          726 g          301 t
ORIGIN      
        1 cgtcgccacg ctcatcgcgt cgctggcgat gcacctgttg ctgtggccga tcggcgaccg
       61 cctgatcaag ctgtcctgga gcgacccgcc gccgccgccg ggcgagggct tcatgcaggt
      121 cgcgctcgtc gacgaggaca agccacccga ccccgacgac gaggtccccc gcgagaccga
      181 cccgcccggc aagctgatca agcaggaccg ggtcaaaaag gaggccgtgc ccgacgacgc
      241 caaacacgtc gccgagttcg accagaaggt cgaggacgag acccgggccg cgcgcggccg
      301 cgccaagcca ggcgccgcgc cgaccacccc cggcacctcg cccgacgcca acaacctccc
      361 cgcgaccccc aacctgctcg acccgcggcc cgccgagccc aagcaggcct ccggctcgcc
      421 gggcgaggcc gacgagggcc gcggcgaccc ggcgacgccg aaggtcccga gcccgcgctc
      481 gctgatgccg ctgctcccgg agatgggccc atccggtcgc ccgggcgtcc gcgggaatcc
      541 ctccgagatg tcgccggccg acccgggcca gcacggcagc atggacgaca tccaggacgt
      601 cgaggagggc gacgtcaacc agctcaactc gcggcgctgg aagtacgcga gttttttcaa
      661 tcgcgtgcgc gaccaggtcg cccagcactg gcaccccgag gtcgtgcacg ccgcgcgtga
      721 cccggacggc agccgctacg gcatcaagac gcgggtgacc cgcctgctga tccgcctcaa
      781 cccggacggc agtttaaata agatcaagat cgaccagcac tccggggtgg atttcctcga
      841 cgaggaggcg atccgggccg tgcgcgccgc ccaccccttc gcaaatccgc ccgcccagct
      901 ggtcgacgcc gagacgggct ttatcgactt cggcttcggc ttcatcttcg agatcgaaag
      961 cggcaagccg cagatcttcc gctaccgccg ctgagatcct ggccttttgg gcgccgcggg
     1021 tgacgtatga tggccgcgcg gccatgacct tgatcaagcg cctgttcgtc gtcgtgttgc
     1081 tggcggtcgc cctggccctc gcgtacttgg ccctcgcgcc ctcggcggtc gacccggtgc
     1141 agtggaaccc gccgccggtg cccgagatgg ccggcccgct ggcgcccaac gcccacctcg
     1201 cgctcgccga gctgatcgcg cagggccacg tcgacggccc cgaggacgtc gagaccgacg
     1261 cgcagggccg cctgtacgcg ggcaccaacg ccggcacggt cgtccgcgtc gagaacggca
     1321 aggtggaggt gttcgcaacc accggcggcc gcccgctcgg cctcgacttc gccccggacg
     1381 gccagctgat cgtcgccgac gcgatcaaag gcctgctggc gatctccccc gacggcaagc
     1441 agatcacgac cctcgccgac gcggccgacg gcgtggcctt cggcttcacc gatgacgtcg
     1501 cggtcgcccg cgacggcacg atctacttca gcgacgcgag cgacaagttc ggcttcggcg
     1561 atcacatgta cgacctgctc gagggccgcc cgcacggccg cctgatgcag tacgacccga
     1621 cgagcaagac caccaaggtg ctgctcaccg acctgtattt cgccaacggc gtcgcgctcg
     1681 cgggcgacga gagctttgta atcgtcagcg agacctaccg ctatcgcctg acccgctact
     1741 ggctcaaggg gcccaaggcc ggcagcaccg acgtgctcgc cgagggcctg cccggcttcc
     1801 ccgacggcgt gtcccagagc ggccgcggca ccttctgggt ggcgatcttc accgtccgca
     1861 acaaggccgg cgacttcctg gcgccgcggc ccctgctcaa gcgcatggtc gccaacttgc
     1921 cgagattcct gtggcccaag cctgacccgt acgggctgat catcgagctc gatgagcagg
     1981 ggaacgtcct gcgcagcttg cacgacgcga gcggcgccta tgtggaacag gtgacgtcgg
     2041 tgcacgagcg ggacgggaag ctgtatctcg ggcatctgca tcgggaccgt atcactcgcg
     2101 tcgggctgtg attcggatcg gtgggcgccg gagcggggac gtgccgaacc tggcgtgcgc
     2161 acacatggcg cgtgcgggac atatggcgcg tgcggg
//
LOCUS       DASWNJ010001018         2098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_266690, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001018 DASWNJ010000000
VERSION     DASWNJ010001018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266690"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VGB85_17200"
     CDS             <1..248
                     /locus_tag="VGB85_17200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135827.1"
                     /translation="GEAETGGIATIAMRVAVHCPACGGDPAIPCDRCKGVRTVEELFS
                     AWLTVPPGVADGTILRPSVQMQWVVRPVQFRVRLREA"
     gene            complement(267..950)
                     /locus_tag="VGB85_17205"
     CDS             complement(267..950)
                     /locus_tag="VGB85_17205"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEY0135828.1"
                     /translation="MCGRFTLTTPDIEALARVVSAEIDPRLLDLHQPRYNIAPTSVVP
                     LLVPAGERRILEPGVWGLPSPFGDDKRPGGFINARAETAAKLPTFRDAFARGRCGVMT
                     DGFFEWTGPKSQRRPLWFRRHDGAPMVLAGLYRDHSDPETGEVTRRFAILTTRANATL
                     APHHDRMPVILPLPGLERWFGASKPNTLGDLLEPAPDDLLSTTPVSTRVNSVRFDDEG
                     CIAPYAEVA"
     gene            998..>2098
                     /locus_tag="VGB85_17210"
     CDS             998..>2098
                     /locus_tag="VGB85_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135829.1"
                     /translation="MTGWHDGAGGMTLAVRRVLSRFEVRLFLSACIVVSLLPLPEVRE
                     LDGLFLLVFGVEFLLRAAVVFRGEGEDADERGWRWPTRGALLLLSFDALALVSFLPLV
                     AHDTPWMRLLRVIRLVALLSYWAPVMQDLRAVLLRRERSRQVLVMGAMVGLLSFIGAL
                     ALEQLEGGVGTVDYDEDGMITHSDRRFFVHLWWAFRQIQDPGNMLAAPGSAGAVIVSM
                     VLTVFGLLLVSFLIGLGTDVVREVMTVSHLRSPGLVGHTVIVHVNAATDPLLQELVRY
                     YQKLIPAGSLSRRWLRQLIENARRVVLGSRYVLVGRSEERPAFLRRPELAHIVYRPVT
                     TADQDFMVRADVARAQRVVILADLEVANPDADT"
BASE COUNT          264 a          602 c          871 g          361 t
ORIGIN      
        1 cgggggaggc cgaaacggga gggatcgcca cgatcgccat gcgcgtcgcg gtgcactgcc
       61 cggcgtgtgg cggggatccg gcgatcccct gcgatcgctg caagggcgtg cgtacggtcg
      121 aggagctgtt cagcgcctgg ctgacggtgc cgcccggggt cgcggacggc acgatcctgc
      181 ggccctcggt gcagatgcaa tgggtggtgc gtcctgtgca gtttcgggtg cggctgcgcg
      241 aggcgtgagc cggcggatga tgcggatcac gcgacctcgg cgtagggggc gatgcagccc
      301 tcgtcgtcga agcgcacgct gttgacgcgg gtcgagaccg gggtggtcga gagcaggtcg
      361 tcgggggcgg gctcgagcaa gtcgccgaga gtgtttggtt tgctggcgcc gaaccaccgc
      421 tcgaggccgg gcagcggcaa gatgacgggc atgcgatcgt ggtgcggggc gagggtcgcg
      481 ttggcccgcg tggtgaggat cgcaaaccgg cgggtgacct cgccggtctc ggggtcgctg
      541 tgatcgcggt agaggccggc gaggaccatc ggtgcgccgt cgtgccggcg aaaccatagc
      601 gggcggcgct gcgacttggg gccggtccac tcgaagaagc cgtcggtcat gacgccgcag
      661 cggccgcggg cgaaggcgtc gcgaaaggtc gggagcttgg cggccgtctc ggcgcgcgcg
      721 ttgatgaagc cgccgggacg cttgtcgtcg ccgaaggggc tgggcaggcc ccacacgccc
      781 ggctcgagga tcctgcgctc accggcgggg acgagcagcg ggacgacgga ggtcggggcg
      841 atgttgtagc ggggctggtg gaggtcgagc aggcgcgggt cgatctccgc cgagacgacg
      901 cgggcgaggg cctcgatgtc gggggtggtg agggtgaagc ggccgcacat gcgagagagt
      961 gtcgcgcggg tgggggcagt tggctatcct cgcggccgtg acgggctggc acgacggggc
     1021 gggtgggatg acgctggcgg tgcgccgggt gctgtctcgc ttcgaggtgc ggctgtttct
     1081 cagcgcgtgc atcgtcgtca gcctgcttcc gctgccggag gtgcgtgagc tcgacgggct
     1141 gttcctgctg gtgttcgggg tcgagttctt gctgcgggcg gcggtggtat ttcgcggcga
     1201 gggcgaggac gccgacgagc gcggctggcg ctggccgacg cgcggcgcgc tgctgctgct
     1261 gagcttcgac gcgctggcgc tggtgtcgtt cctgccgttg gtcgctcacg acaccccgtg
     1321 gatgcgcctg ctgcgggtga tccgtctcgt cgcgctgctg agctactggg cgccggtgat
     1381 gcaggacctg cgggcggtgc tgctgcggcg cgagcggtcg cggcaggtgc tggtgatggg
     1441 ggcgatggtc gggctgctgt cgttcatcgg ggcgctggcg ctcgagcagc tcgagggcgg
     1501 cgtgggcacc gtcgattacg acgaggacgg catgatcacg cacagcgatc gccggttctt
     1561 cgtgcacctg tggtgggcgt ttcgccagat ccaggacccg ggcaacatgc tcgcggcgcc
     1621 gggctcggcg ggggcggtga tcgtcagcat ggtgctgacg gtgttcgggc tgttgctggt
     1681 cagtttttta attgggctcg gcaccgatgt cgtgcgcgag gtgatgacgg tcagccacct
     1741 gcgatcgccg gggctggtcg ggcacacggt gatcgtgcac gtcaacgcgg cgactgaccc
     1801 attgctgcag gagctggtcc gttattatca aaagctgatc ccggcgggct cgctgtcgcg
     1861 gcggtggttg cggcaattga tagagaacgc gcgacgggtg gtgctcgggt cgcgctatgt
     1921 gctcgtcggg cgcagcgagg agcgcccggc gttcctgcgc cggccggagc tggcacatat
     1981 cgtatatcga ccggtgacga cggccgacca ggacttcatg gtccgcgccg acgtcgcgcg
     2041 ggcgcagcgg gtggtcatcc tcgcggacct cgaggtggcc aatcccgatg cagatacg
//
LOCUS       DASWNJ010001019         3106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_266697, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001019 DASWNJ010000000
VERSION     DASWNJ010001019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266697"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VGB85_17215"
     CDS             <1..502
                     /locus_tag="VGB85_17215"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY0135830.1"
                     /translation="ERLVEELNAYLHDMVAEIRAEGGVVDKYIGDAIMAVFGVPSEHP
                     DDAARAIRAADRMRGALVRHNVDRKARGLPPLAHGVGVHYGPMVAGNIGTAERMQYTV
                     IGDAVNLASRLETATKDVAVEMLISDAAVQAALSSGVALPILRVHGPIHVRGHEAPIV
                     VHTSAV"
     gene            complement(506..1027)
                     /locus_tag="VGB85_17220"
     CDS             complement(506..1027)
                     /locus_tag="VGB85_17220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135831.1"
                     /translation="MSRVSYFAALIALACSCTQPPSPPPAPPSNCSTGGPGPAPGPPQ
                     PKITEGIAGVICFWEGNFMSTLEPDERGTVKPVPRTIEIHAPTRSADGVGGDGATFWG
                     SVSTPLIATANSGDDGWFEVAVPPGTYSLLVRESGRLYANSFNGEGIIAPIEVTAGKV
                     ARTQFDINYRAVE"
     gene            complement(1080..2879)
                     /locus_tag="VGB85_17225"
     CDS             complement(1080..2879)
                     /locus_tag="VGB85_17225"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135832.1"
                     /translation="MRRACLLASLLLVACSDDTGATTEPASTSTTSTTSTGAPDSSST
                     APPTTTVESTTLETSEPTSTGTIDPTTSGSTGTSTGEPLDPVCDQFIKPCEQCSCTDA
                     GWSCECPPLLPEAGFIEIEPVKFTIGQGNKAQGRTSSPARLFYSFRPAEPGVEGPLFV
                     LFNGGPGSSTGTLMAFGTGPVALTPDLIKNPGSWATLGDLLYIDARGTGFSYNLAENP
                     ADLEVRKAEFGISNYNSYLDAADFVRVLLRFIVAHPQLSTREFVIVGESYGGMRATIM
                     LELLLGFPNYDEGGPGLYDDAALVDEIETFLATRDPDVDEWTPAEISKIFPRQILIQP
                     SLGGLQRTIAGQLLDLPDSPIFKIAQELGATFKPCSEQGADCLPWQNAVQFIENTVAR
                     SRYDLDAPNTWLTDLFAAKKAGLSDLLKLEAVLGLPPASVPLLPAEARGGAFRMIGLG
                     SYPADGGTISMLGALEKWDRYYIPFLAESNNAIRSALADHVGIGPDDPHFAALFLHNL
                     AHVDTFITAAERDIAIYAPSIPPLLATYTDIVDSVESLPGEFHVFYKDSPFPDEPAPG
                     TRVVRFPTYDASHAVSLDQPTALRDDVAAWLAP"
     gene            complement(2905..>3106)
                     /locus_tag="VGB85_17230"
     CDS             complement(2905..>3106)
                     /locus_tag="VGB85_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135833.1"
                     /translation="EHVLVLQGSYQDSDGQSARAGDLVHMPAGSSHTFTAGPDTDLIY
                     AVVVHGVEIEGIDPDAIRPPRA"
BASE COUNT          495 a          877 c         1212 g          522 t
ORIGIN      
        1 cgagcgactt gtggaggagc tcaacgcgta tctgcacgac atggtcgcgg agatccgggc
       61 cgagggtggg gtggtcgaca agtatatcgg tgacgcgatc atggcggtgt tcggggtgcc
      121 gagcgagcac ccggacgacg ctgcgcgggc gatccgggcc gcggatcgca tgcgcggggc
      181 gctggtgcgc cataacgtcg atcgcaaggc gcgcgggctg ccgccgctgg cccacggggt
      241 cggcgtgcat tatgggccga tggtcgccgg taacatcggc accgcggagc gcatgcaata
      301 cacggtgatc ggggacgccg tgaacctcgc cagccggctc gagacggcga ccaaggatgt
      361 cgcggtcgag atgctgatct cggatgccgc cgtgcaggca gcgctgtcgt cgggagtcgc
      421 gttgcccatt ttaagggtgc acgggccgat ccacgtgcgg gggcacgagg cgccgatcgt
      481 cgtgcacacg agtgcggtat gatgattatt cgaccgccct ataattaatg tcgaactggg
      541 tgcgagcgac cttgccggcg gtgacctcga tcggggcgat gatgccctcg ccgttgaagc
      601 tgttggcgta gaggcggcca gattcgcgga cgagcaggct gtaggtgccg ggcgggacgg
      661 cgacctcgaa ccagccgtca tcgcccgagt tggcggtggc gatcaagggt gtcgacacgg
      721 agccccagaa ggtcgcaccg tcgccgccga cgccgtccgc gctgcgggtc ggggcgtgga
      781 tctcgatcgt gcggggaacc ggcttgaccg tccctctttc atcgggttcc agcgtggaca
      841 tgaaattgcc ctcccagaag cagatgacgc cggcgatgcc ctcggtgatc ttcggctgcg
      901 gcgggccggg agccgggccc gggccgccgg tgctgcagtt ggatgggggc gctggaggcg
      961 gggacggcgg ctgcgtacag ctacacgcga gggcgatcag ggcggcaaaa tacgagaccc
     1021 gggacatcgc tgaaattgta tgccccggct tcgcgctgtg tcaaacgcgc gcgcgcacct
     1081 cagggcgcga gccaggcggc gacatcgtcg cgcagtgcgg tcggctggtc gagactgacg
     1141 gcgtgcgagg cgtcgtaggt cggaaaccga acgacgcggg tgccgggcgc gggctcgtcg
     1201 gggaaggggg aatctttata aaagacgtgg aattcgcccg ggagagattc gacggagtcg
     1261 acgatatccg tataggtggc gagcagcggc gggatcgatg gggcgtagat tgcgatgtcg
     1321 cgctctgcgg ctgttataaa ggtgtcgacg tgggcgaggt tatggagaaa gagggcggcg
     1381 aagtgtgggt cgtcggggcc gatgccgacg tggtcggcga gcgcggagcg gatggcgttg
     1441 ttggactcgg cgaggaaggg gatgtagtag cggtcccact tctcgagcgc gccgagcatc
     1501 gagatcgtgc caccatcggc cggatagctg ccgaggccga tcatgcgaaa ggcaccgccg
     1561 cgggcctccg cgggcaacag cggcacgctc gcgggcggca ggccgagcac ggcctcgagt
     1621 tttaaaaggt cgctgaggcc agcctttttg gccgcgaaca ggtcggtgag ccaggtgttg
     1681 ggggcgtcga ggtcgtagcg gctgcgggcc acggtgtttt cgatgaattg cacggcgttt
     1741 tgccagggca ggcagtccgc gccctgctcg ctgcacggct tgaaggtcgc gccgagctcc
     1801 tgggcaattt tgaaaattgg cgagtcgggg aggtcgagca gctggccggc gatcgtgcgc
     1861 tgtagtccgc ccaggctcgg ctggatgagg atctggcgcg ggaaaatttt ggaaatctcg
     1921 gcgggggtcc attcgtcgac gtcggggtcg cgggtggcaa ggaaggtctc gatctcgtcc
     1981 accagagcgg cgtcgtcgta gaggccgggg ccgccctcgt cgtagttcgg gaagccgagc
     2041 agcagctcga gcatgatcgt cgcacgcatg ccgccgtagc tctcgccgac gatgacgaac
     2101 tcgcgcgtcg agagctgcgg gtgggcgacg atgaagcgca gcagcacgcg cacgaagtcg
     2161 gcggcgtcga gatagctgtt gtagttactg atcccaaatt cggccttgcg gacctcgagg
     2221 tcggccgggt tctcggcgag gttgtaggag aagccggtgc cgcgcgcgtc gatgtagagc
     2281 aggtcgccga gcgtcgccca cgacccggga tttttaataa ggtccggggt cagcgcgacg
     2341 gggccggtgc cgaaggccat cagggtgccg gtcgacgagc cggggccgcc gttgaacagc
     2401 acgaacaggg gaccctcgac gccgggctcg gcggggcgaa agctataaaa caggcgcgcg
     2461 ggcgaggagg tgcgcccctg ggccttgttg ccctgaccga tcgtgaactt gaccggctcg
     2521 atctcgatga agccggcctc cggcagcagc ggcgggcact cgcaggacca gccggcgtcc
     2581 gtgcagctgc actgctcgca gggtttgata aattggtcgc agacggggtc gagtggctca
     2641 cccgtgctcg tgccggtcga tccgctcgtc gtcgggtcga tggtcccggt ggaggtcggc
     2701 tcgctcgttt cgagggtggt cgactcgacg gtggtcgtcg ggggcgcggt gctcgaggag
     2761 tccggggcgc cggtcgaggt ggtcgaggtg gtcgacgtgc tcgcgggttc ggtggtggct
     2821 ccggtgtcgt cgctgcacgc gacgagcagc aggctcgcta acagacaggc tcgtcgcatg
     2881 gcgagagtgt cgcacgtaag ttatctatgc acgaggcggg cggatcgcgt ccgggtcgat
     2941 gccttcgatc tcgacgccgt ggacgacgac ggcgtagatc aggtcggtgt cggggccggc
     3001 cgtgaaggtg tgcgagctgc cggcgggcat gtggacgagg tcgccggcgc gcgcgctctg
     3061 gccgtcgctg tcctgatagc tgccctggag cacgagcacg tgctcc
//
LOCUS       DASWNJ010001020         3232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_230145, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001020 DASWNJ010000000
VERSION     DASWNJ010001020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230145"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1500
                     /locus_tag="VGB85_17235"
     CDS             <1..1500
                     /locus_tag="VGB85_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135834.1"
                     /translation="TTDASDLFDTFLDALPPELRPIYVCSACRTFMRRFGGVVRVTPT
                     GETVSVMWDPEAVAEPYVAAVRALASAVARAPIVDVFLATEPVWGQPVTGEWHHFAVT
                     PPAALIFKPSAIQTTGQRVAEKREDYAILLRGLTEFPADLVINAHTLLTTGSLYRSEK
                     CIGVARWLMDLHEQRRGALDERRRTNLTWVAVASAPAGFCHVRSSMIGTLLEDLAAEL
                     PFEEIKAKFAAKMHPLQYQRPTAAPSAGNIAQAEKIIEQLRTAGALERRFARLADIVA
                     VWKPRPVETPQGKGVFSHLKPKQAQARKAVVPPPVTMTWDKFSRTVLPDAESIEYHVP
                     AGKESYLGMVTAKHADAPPIVQWDSESQRNPVTWYVYHGGSAPADWNLKAGVRHPVTA
                     VTLAPWMWHATDKNRSSHQGQSVVFILDGARDMKYSKGAAFFPEFLRSEYHSIRSTME
                     AYAKSAVVEGKDEAEACGLVLTKGGNWNHVFRVTSKNGVQVDYKIDRWD"
     gene            1500..2069
                     /locus_tag="VGB85_17240"
     CDS             1500..2069
                     /locus_tag="VGB85_17240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135835.1"
                     /translation="MSGDTALKLGMLTAILLVATLGAMLGYAVTWLLAKVLPRPAVIP
                     LGIVLCLAGAAIAVHVVFPDILRSIRDDASAPTYHFQVPSGFTGRFTLVHDPAGVRLV
                     EDGRRVEIPVPPDRMLRVQPSPALEHTDPRLTATRDGAPTTIIADESGQWGGIRYQTF
                     YVGTYDDRARDPGSSPPEPILSLKRDADR"
     gene            complement(2047..3081)
                     /locus_tag="VGB85_17245"
     CDS             complement(2047..3081)
                     /locus_tag="VGB85_17245"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0135836.1"
                     /translation="MAERPSIRINDVDFVWDTERGDLSVTGLNSVLFWTDPSMYRLLA
                     PLVEELGVDLFRLLVAESSSVGTDADYDFMVKLGVDFVPGFLAWGGLVATAGWGRFEV
                     MAIDPKACTAHVRVHHPWELQMQRDHALRWGCPFLQGKTIGIFTHAFGRPCWAEEECS
                     MDPERPCLDMHIHPSDRILDRELAVLRDRLFDQREGELRRRIDEATTELRSKIAVIEE
                     QRQLIARLTYPILQVWDGVLAAVLVGELNDAALTDLGYALLQRVQATRSRHVILDCTG
                     VPALGATQAAGLARLVSALRLLGTQPTLVGISPEVAQSLADETGALSGTRTLQSLADA
                     LQRVIGLRRA"
BASE COUNT          511 a         1162 c         1076 g          483 t
ORIGIN      
        1 acgaccgacg cgtcggacct cttcgacacc ttcctcgacg ccctgccgcc ggagctgcgc
       61 ccgatctacg tgtgctcggc ctgccggacc ttcatgcgtc gcttcggcgg cgtcgtccgc
      121 gtcaccccga ccggcgagac cgtctcggtg atgtgggacc ccgaggcggt cgccgagccc
      181 tacgtcgcgg ccgtgcgcgc gctcgcgtcc gcggtcgcgc gcgcgccgat cgtcgatgtc
      241 ttcctcgcca ccgagccggt gtggggtcag cccgtgacgg gcgaatggca tcacttcgcc
      301 gtcacgcccc ccgccgcctt aatttttaaa ccctccgcga tccagaccac gggtcagcgg
      361 gtcgcggaga agcgcgagga ctacgcgatc ttgctgcgcg gcctcacgga gttcccggcc
      421 gacctcgtga tcaacgcgca caccttgctg accaccggct cgctgtatcg ctcggagaaa
      481 tgcatcggcg tcgccaggtg gttgatggac ctgcacgagc agcgccgcgg cgcgctcgac
      541 gagcgccgtc gcaccaacct gacctgggtc gcggtggcga gcgccccggc gggcttttgc
      601 cacgtgcgca gctcgatgat cggcacattg ctcgaggacc tcgccgccga gctgcccttc
      661 gaggagatca aggccaagtt cgcggccaag atgcaccctc ttcaatatca gcgcccgacc
      721 gcggcgccga gcgccggcaa catcgcccag gccgagaaga tcatcgagca gctccggacc
      781 gccggcgcgc tcgagcgccg cttcgccagg ctcgccgaca tcgtcgccgt ctggaagccg
      841 aggcccgtcg agaccccgca gggcaagggc gtgttctccc acttgaaacc caagcaggcg
      901 caggcgcgca aggccgtggt cccgccgccg gtcacgatga cgtgggacaa gttctcgcgc
      961 acggtcctgc ccgacgccga gtcgatcgaa taccacgtgc ccgccggcaa ggagtcgtac
     1021 ctcggcatgg tgacggccaa gcacgccgac gccccgccga tcgttcaatg ggacagtgaa
     1081 tcgcagcgca acccggtcac ctggtacgtc tatcacggcg gctcggcgcc ggccgactgg
     1141 aacctcaagg ccggcgtccg tcacccggtc accgcggtga cgctggcgcc gtggatgtgg
     1201 cacgcgaccg acaaaaacag atcctcgcat cagggccaga gcgtcgtgtt catcctcgac
     1261 ggcgcgaggg acatgaaata cagcaagggc gccgcgttct tccccgagtt cctcaggagc
     1321 gaataccact ccattcgtag cacgatggag gcctatgcga agtccgcggt cgtcgagggc
     1381 aaggacgagg ccgaggcctg cggcctggtg ctcaccaagg gcggcaactg gaatcacgtg
     1441 ttccgcgtga cttccaagaa cggcgtgcag gtggattaca agatcgacag gtgggactga
     1501 tgagcggtga tactgccctg aagctgggca tgctgacggc gatcctgctg gtcgccaccc
     1561 tcggcgccat gctcgggtac gccgtgacct ggctgctcgc caaggtcttg ccgcgcccgg
     1621 ccgtgatccc gctaggaatc gttctatgcc tcgccggcgc cgcgatcgcg gtccacgtcg
     1681 tgttcccgga catcctgcga tcgatccgcg acgacgcgag cgcgccgacc tatcacttcc
     1741 aggtgccgtc cggcttcacg gggcgcttca ccctcgtcca cgatccggcg ggcgtgcgcc
     1801 tggtcgagga cggccgacgc gtcgagatcc ccgtgccccc ggatcgcatg ctgcgggtcc
     1861 agccgagccc cgcgctcgag cacacggacc cgcggctgac tgccacccgc gacggcgcgc
     1921 cgaccacgat catcgccgac gagtccgggc aatggggcgg catccgatat cagacctttt
     1981 atgtcggtac gtacgacgat cgcgccaggg acccgggctc gagcccgccc gagccgatcc
     2041 tgagcctcaa gcgcgacgca gaccgatgac gcgctgcagc gcgtcggcga ggctctgcag
     2101 cgtgcgcgtg ccggacagcg cgcccgtctc gtcggcgagg ctctgcgcga cctccggtga
     2161 gatgccgacc agcgtcggct gggttcccaa caatcgcagc gccgagacca ggcgcgcgag
     2221 gccggcggcc tgcgtcgcgc cgagggccgg cacgccggtg cagtcgagga tgacgtgccg
     2281 cgagcgcgtc gcctgcaccc gttgcagcag cgcgtagccg aggtcggtca gcgcggcgtc
     2341 gttgagctcg ccgacgagca ccgccgcgag cacgccgtcc cacacctgca ggatcgggta
     2401 ggtcaggcga gcgatcagct ggcgctgctc ctcgatcacg gcgatcttgc tgcgcagctc
     2461 ggtcgtcgcc tcgtcgatcc gccggcgcag ctcgccctcg cgctggtcga acaggcggtc
     2521 gcgcagcacc gcgagctcgc ggtcgaggat ccggtccgac gggtggatgt gcatgtcgag
     2581 gcacggacgc tccgggtcca tcgagcactc ctcctcggcc cagcacgggc gcccgaacgc
     2641 atgcgtgaag atgccgatcg tcttgccctg caagaacggg cagccccagc gcagcgcgtg
     2701 gtcgcgttgc atctgcagct cccacgggtg atgcacgcgc acgtgggcgg tgcacgcctt
     2761 cgggtcgatc gccatgacct cgaagcggcc ccagccggcg gtcgctacca ggccgcccca
     2821 cgccaggaag ccgggcacga agtcgacgcc gagcttgacc atgaagtcgt agtccgcgtc
     2881 ggtgccgacg ctcgagctct cggcgacgag caggcgaaac aggtcgacgc cgagctcctc
     2941 gaccagcggc gcgagcagtc ggtacatcga tgggtcggtc cagaacagga ccgagttcag
     3001 gccggtgacg ctgaggtcgc cgcgctcggt gtcccacacg aagtcgacat cgttgatgcg
     3061 gatactcgga cgctctgcca cggaccgcga gggtacgctg ttttgcggct cgcagcggga
     3121 tccgcggccg cgcgccatcg cccgcgagtc cgcgatcgcc ggcctgcttt gcataccgcg
     3181 ccgcgaaacc acggtatatc ccgcgcacgt gagccgcgcc ctcctcgcac tg
//
LOCUS       DASWNJ010001021         1839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_123792, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001021 DASWNJ010000000
VERSION     DASWNJ010001021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123792"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VGB85_17250"
     CDS             complement(<1..373)
                     /locus_tag="VGB85_17250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135837.1"
                     /translation="MRSDLRLRLEAAAAARSAAPDRLLDELLERGLGEVEAEIAYERA
                     VAGGGAFLASGMSAAGPELLDAIVPPALTAVLPGRPGWRGRGRGYVPEPKAPRLFRAR
                     RDDGIEVAAQIAFGPEPGPTLS"
     gene            complement(384..1454)
                     /gene="recA"
                     /locus_tag="VGB85_17255"
     CDS             complement(384..1454)
                     /gene="recA"
                     /locus_tag="VGB85_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEY0135838.1"
                     /translation="MADTQVSRRDGNDRQRALDAAIAQIERNFGKGSVMRLGHGGERS
                     EIEVVSTGSLGLDIALGVGGLPRGRIIEIYGPESSGKTTLTLHVLAEAQKVGGTCAFI
                     DAEHALDPSYAKKLGVDIDALLISQPDTGEQALEIADTLVRSGAVDVIVVDSVAALVP
                     KAELEGEMGDTHVGLQARLMSQALRKLTASISRSKCMLIFTNQIRSKIGVMFGSPETT
                     TGGNALKFYASIRLDVRRIGQVKDKDEVVGNQTKVKVVKNKVAPPFRVVEFDIVYGEG
                     ISKSGEIIDLGLKAGVVEKSGSWFSWNGQRIGQGRENAKTYMREHPEAAAEIERTIRA
                     QAVPGGGSAGIDLLEQDMTVDE"
BASE COUNT          254 a          651 c          628 g          306 t
ORIGIN      
        1 ccgagagggt agggccgggc tccgggccga acgcgatctg cgcggcgacc tcgatcccgt
       61 cgtcgcgtcg tgcccggaac agccgagggg ccttcggctc gggcacgtag ccccgcccgc
      121 gccctcgcca accggggcgg cccggcagca cggccgtgag cgccgggggc acgatcgcgt
      181 ccagaagctc aggtccggcg gcgctcatcc ctgacgcgag gaaagcacct ccgccggcga
      241 ccgcccgctc gtaggcgatc tcggcctcga cctcccccaa gccgcgctca agcagctcgt
      301 cgagcaggcg gtcgggtgcg gcggacctcg ccgccgcggc ggcctcgaga cgaagccgga
      361 ggtccgatcg cacgcgggcg ggcctactcg tcgaccgtca tgtcctgctc gagcaggtcg
      421 atgcccgccg agccgccgcc ggggacggcc tgggcgcgga tggtgcgctc gatctcggct
      481 gcggcctcgg ggtgctcgcg catgtaggtc ttggcgttct cgcggccctg gccgatgcgc
      541 tggccgttcc aggagaacca ggagcccgac ttctcgacca cgccggcctt caggccgagg
      601 tcgatgatct cgccggactt cgagatgccc tcgccgtaga cgatgtcgaa ctcgacgacg
      661 cggaacggcg gggcgacctt gttcttgacc accttcacct tggtctggtt gccgacgacc
      721 tcgtccttgt ccttcacctg cccgatgcgg cgcacgtcga ggcggatcga ggcgtagaac
      781 ttcagcgcgt tgccgcccgt ggtggtctcg gggctgccga acatcacgcc gatcttgctg
      841 cggatctggt tggtgaagat cagcatgcac ttggagcggc tgatgctcgc ggtgagcttc
      901 ctgagcgcct ggctcatcag ccgcgcctgg aggccgacgt gggtgtcgcc catctcgccc
      961 tcgagctcgg ccttgggcac caatgccgcc accgagtcga ccacgatcac gtcgacggca
     1021 cccgagcgca ccagggtgtc ggcgatctcg agcgcctgct cgccggtgtc gggctgggag
     1081 atgagcagcg cgtcgatgtc gacgccgagc ttcttggcgt aggaggggtc gagcgcgtgc
     1141 tccgcgtcga tgaaggcgca ggtgccgccg accttctgcg cctcggccag gacgtgcagg
     1201 gtcaacgtcg tcttgccgga gctctccggc ccgtagatct cgatgatgcg cccgcgcggc
     1261 aggccgccca cccccaacgc gatgtcgagg ccgagcgagc ccgtggagac gacctcgatc
     1321 tcggaccgct cgccgccgtg gccgagccgc atgaccgagc ccttgccgaa gttgcgctcg
     1381 atctgcgcga tcgccgcgtc cagcgcccgc tgccggtcgt tgccgtcgcg ccggctgacc
     1441 tgggtatcgg ccatgtctgc tgtctcgctc gctgggtggg acgccctcgg gcaggtggac
     1501 tgatataggc atccggccgg agcaccggaa ggccgtgttc ctgaaatgtt ttccgacact
     1561 tagcgcttga gccacccccg atcgcccgcg tgccatcagc gtatggtacg ctccgggttc
     1621 ctgtcccgct agggccctga agcggaggcc tcccgcccca agactgcggc ttccgacagc
     1681 ccaaactaag ggcgccacct tacgggcgat cccctaatcg cggtcgctcg aggggagcgc
     1741 ctcaggatag agtatcgccg tgccgggtat ggcctttgcc gtccaatcat cgcacgtcgc
     1801 gtcgaactgg agggtcgcct gcatctccag cttaccggt
//
LOCUS       DASWNJ010001022         3787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_88611, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001022 DASWNJ010000000
VERSION     DASWNJ010001022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88611"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..472)
                     /locus_tag="VGB85_17260"
     CDS             complement(116..472)
                     /locus_tag="VGB85_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135839.1"
                     /translation="MTKQIKIVLAALSLTMIAGACSGGAAGGNSLCEKAAAPLSGIVL
                     LEVKDAGDDPAILKTFKAELAAECTAQKTEETAKDALECYDKNASALGYRIFKTCPEQ
                     PGKALIAAVVAKHGAK"
     gene            651..1571
                     /locus_tag="VGB85_17265"
     CDS             651..1571
                     /locus_tag="VGB85_17265"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0135840.1"
                     /translation="MADLFAGVLPFIAVAEAQSFRRAAEQLGVSTAAVSKAIKRLETE
                     LGVRLLERSSRKVALSAEGQALLVRAREAVALLRAGREAAAHGAREPRGSVTVSVPFT
                     LSQQVTPALARLTQQYPSLAVALRFSDRMARFVDEQVDVAVRMGTLADSSLVARMLFR
                     PRWMTVAAPAYLGRRGAPRRPEDLARHELLAFVMPGGQRRDWLFRVAPGDEATRELTP
                     AGAIDASFGDALVHAAVSGVGVAQVLDFMAAEAVARGELIEVLSELAAAGPPVYAVCL
                     PGRQGTPRVRAVLGALADAFKPIPRVGRTA"
     gene            complement(1575..2732)
                     /locus_tag="VGB85_17270"
     CDS             complement(1575..2732)
                     /locus_tag="VGB85_17270"
                     /inference="COORDINATES: protein motif:HMM:NF021466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="App1 family protein"
                     /protein_id="HEY0135841.1"
                     /translation="MRRDLLPLVLLACDRGAPTPAKPTVAEPAATEPAAPTPTALPSP
                     PVPAASGRTSNLKTDEEARVFPAIGRRDGATWSVRVHAWVYEPEENSSSRTATVEALR
                     KALELPEDAPSTDIFRARARPFLVDNESGKHLLVRIGGQEHELAGTLMDGHSHTDLEL
                     RAEGLVPDAAGVVAIDVIPRTGDPRQLSGVAILLADTGTSVVSDIDDTVKISEVRDKK
                     KLLARTFLQEFEPVPGMADAYRRWADAGAAFHYVSASPWQLYDPIDGFFRREKFPQGS
                     VHLKQFRWKDSSFLALFQDPVGYKQPIISGLIKQFPQRRFVLVGDSGEQDPEVYGAIY
                     REFPTQVTAIHIRDVTGEDREAARYKTAFTKVPAERWSIFTDATTLPASLP"
     gene            2989..>3787
                     /locus_tag="VGB85_17275"
     CDS             2989..>3787
                     /locus_tag="VGB85_17275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135842.1"
                     /translation="MASLGLAATSAWLVPGCGGDETASSASASGSSSSGDVVQTTTGT
                     PTTGPDTSSVTGDPPTTGNSDAMTMGTTTDDPMSGSTGEPTTGTTGEEPFCGDGSVDP
                     GETCDDGDDNGPGQACTATCQANACGDGDVGPGETCDDGADNGDMNSCKADCTDNVCG
                     DGAVGPGEGCDDGNLLDDDECTSACALATCGDGKVAPTEQCDDGDPDNTDACTDACTN
                     AACSDGFVQAGAGEECDAGPDNADNGACTTACKANVCGDGAVWNTGEG"
BASE COUNT          524 a         1279 c         1435 g          549 t
ORIGIN      
        1 ctcggcaagc cggagcgcgt ggtcgacgac ggcggcacga tctacgtggt gccgccgatg
       61 gtcgtcgagg gcttccactt cgcgtcggtc tccgacgcgg tgtgaaatca ctcgatcact
      121 tggcgccgtg cttggcgacg accgcggcga tcagcgcctt gccgggttgc tccgggcagg
      181 tcttgaagat ccggtagccg agcgccgagg cgttcttgtc ataacactcg agcgcgtcct
      241 tggccgtctc ctcggtcttc tgcgcggtgc actcggcggc gagctcggcc ttgaaggtct
      301 tgaggatcgc cgggtcgtcg cccgcgtcct tgacctcgag cagcacgatg ccggacagcg
      361 gcgcggcggc cttctcacac agggagttgc cgccggcggc cccacccgaa caggcgcccg
      421 cgatcatcgt cagcgacagg gcagcgagca cgatcttgat ctgcttggtc atgtttctcc
      481 gttgggtcgg ccgaggtggc ccgcagcgcg cgggtctcga agtcgggcac ccgcgcgcat
      541 cctaactcgt aacgggcacg gccgcgccca ggaaaacttt gcgtcgtcaa ccggaacagc
      601 ggtcacgggc ggggctcgcg ggatcgtcaa cattcggtta atcgttggac atggccgacc
      661 tgttcgccgg ggtgctgccc ttcatcgccg tcgccgaggc gcagagcttt cgtcgcgccg
      721 ccgagcagct cggcgtgtcg acggcggcgg tgtcgaaggc gatcaagcgg ctcgagacgg
      781 agctcggcgt gcggctgctc gagcgcagct cgcgaaaggt ggcgctcagc gccgagggcc
      841 aggcgctgct ggtgcgcgcg cgcgaggccg tcgccctgct gcgcgccggt cgcgaggcgg
      901 cggcccacgg cgcccgcgag ccgcgtggca gcgtcaccgt gtcggtgccg ttcacgctgt
      961 cgcagcaggt gacgcccgcg ctggcccggc tgacgcagca atatccctcg ctcgcggtgg
     1021 cgctgcgctt ctcggatcgc atggcccgct tcgtcgacga gcaggtcgac gtcgcggtgc
     1081 gcatgggcac gctcgccgac tcgagcctcg tcgcacgaat gctgtttcgc ccgcgctgga
     1141 tgaccgtcgc ggcgccggcg tacctcgggc gtcgcggcgc gccccgccgg cccgaggacc
     1201 tcgcgcgcca cgagctgctc gccttcgtga tgccgggcgg gcagcgccgc gactggctgt
     1261 ttcgcgtcgc gcccggcgac gaggcgacgc gcgagctgac gcccgccggc gccatcgatg
     1321 cctcgttcgg cgacgcgctc gtgcacgccg cagtgagcgg cgtcggggtc gcgcaggtgc
     1381 tcgacttcat ggccgccgag gcggtcgcgc gcggcgagct gatcgaggtc ctgtccgagc
     1441 tcgccgccgc cggtccgccc gtctacgcgg tgtgcctgcc gggtcgacag ggcacgccgc
     1501 gcgtgcgtgc ggtgctcggg gcgctggccg acgcgttcaa gccgatcccg cgggtcggtc
     1561 gcacggcatg accttcatgg caagctcgcg ggcagcgtcg tcgcgtcggt gaagatcgac
     1621 cagcgctccg cggggacttt agtaaaggcc gtcttgtagc gcgcggcctc gcggtcctcg
     1681 ccggtgacgt cgcggatgtg gatcgcggtc acctgggtcg ggaactcgcg atagatcgcc
     1741 ccgtagacct ccgggtcctg ctcgccggag tcgccgacga gcacgaagcg gcgctgcggg
     1801 aactgtttga tcaggccgga gataatcggc tgcttgtagc cgaccgggtc ctggaacagc
     1861 gccaggaagc tcgagtcctt ccagcgaaac tgcttgaggt gcacgctgcc ctgcgggaac
     1921 ttctcgcgcc tgaaaaaacc gtcgatcggg tcgtacagct gccacgggct cgcggacacg
     1981 tagtggaacg cggcgccggc gtcggcccag cggcggtacg cgtcggccat gccggggacc
     2041 ggctcgaatt cctgcaaaaa ggtgcgcgcg agcagcttct tcttgtcgcg cacctcgctg
     2101 atcttcacgg tgtcgtcgat gtcggagacg accgaggtgc cggtgtccgc gagcaggatc
     2161 gccacgccgc tcaactgccg cgggtcgccc gttcgcggga tgacgtcgat cgccacgacg
     2221 ccggccgcgt cgggcaccag accctccgcg cggagctcga ggtcggtgtg gctgtggccg
     2281 tccatcagcg tgcccgccag ctcgtgctcc tggccgccga tgcggaccag caggtgcttg
     2341 ccggactcgt tgtcgaccag gaaggggcgc gcgcgggcgc ggaagatgtc ggtcgagggg
     2401 gcgtcctcgg gcagctcgag cgccttgcgc agcgcctcga cggtcgccgt gcgactggac
     2461 gagttctcct ccggctcgta gacccacgcg tgcacgcgca ccgaccacgt cgcgccgtcg
     2521 cggcggccga tcgccgggaa cacgcgcgcc tcctcgtcgg tcttgaggtt cgaggtgcga
     2581 ccgctggcgg ctggcacggg cggcgacggc agggcggtcg gcgtcggcgc ggcgggctcg
     2641 gtggccgcgg gctcggcgac cgtcggcttc gcgggcgtcg gcgcgccgcg atcgcaggcg
     2701 agcaggacga ggggcaggag gtcgcgacgc acggcgccac aatagccgga tcaccgggct
     2761 cgtggccggc gatcgtggcc ttcgcggcgc ggtgacatgt cccccgggcc aggtaagccc
     2821 cggcgctgcg cagcgcggcg cgatcgcgcg cggcgcttga tctttgccgg cgaatgcgga
     2881 taaatggcgg tctcgcggcg atgtcgtcgg cgggtggttg gaagtggtgg atgggaaggg
     2941 tggaggcgag cgatgcgttt catgcgatcg atgcgatgtg gtgagatcgt ggcgagcctg
     3001 ggcctggcgg ccacgagcgc gtggctggtg ccgggctgtg gtggcgacga gacggcgagc
     3061 agcgccagcg cgagcggatc gtcgagctcc ggcgatgtcg tgcagacgac gaccgggacg
     3121 ccgacgaccg ggccggacac gtcctcggtg accggcgatc cgccgaccac cggcaactcc
     3181 gacgcgatga cgatgggcac gaccaccgat gacccgatgt cggggtcgac cggcgagccg
     3241 acgaccggca cgaccggcga ggagccgttc tgcggcgacg gcagcgtcga ccccggcgag
     3301 acctgcgacg acggcgacga caacgggccc gggcaggcct gcaccgcgac gtgccaggcc
     3361 aacgcctgcg gcgacggcga cgtcggtccc ggcgagacct gcgacgacgg ggccgacaac
     3421 ggcgacatga acagctgcaa ggccgattgc accgacaacg tgtgcggcga cggtgcggtc
     3481 gggcccggcg agggctgcga cgacggcaac ctgctcgacg acgacgagtg taccagcgcg
     3541 tgcgcgctgg cgacctgcgg cgacggcaag gtcgcgccga ccgagcagtg tgacgacggc
     3601 gaccccgaca acacggacgc gtgcacggac gcgtgcacca acgcggcgtg cagcgacggc
     3661 ttcgtgcagg cgggcgcggg cgaggagtgc gacgcgggcc ccgacaacgc cgacaacggc
     3721 gcgtgcacga ccgcttgcaa ggccaacgtg tgcggcgacg gtgccgtatg gaacaccggc
     3781 gagggcg
//
LOCUS       DASWNJ010001023         4175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_442927, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001023 DASWNJ010000000
VERSION     DASWNJ010001023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442927"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VGB85_17280"
     CDS             <1..331
                     /locus_tag="VGB85_17280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135843.1"
                     /translation="RNLSAGEVQLLAFARVAARAPTLLILDEATASVDSMTEHKVQAA
                     IEELLRGRSVLVIAHRLSTVRRADKILVMQAGEVVEQGTHEALLALGGVYAELYRMGF
                     KEPEPAA"
     gene            639..1067
                     /locus_tag="VGB85_17285"
     CDS             639..1067
                     /locus_tag="VGB85_17285"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY0135844.1"
                     /translation="MAERIWGECTAYRLRVINRAVSRIYDDALRPYRLRIAQLNTLVV
                     VMQTGGLTPNDLSQRMHMDASTVSRNVERMCKSGWLELADIDDARSHEIRITARGMKL
                     IADVAPAWESAQQQAAELLGVQVSKAIARGGDKLVADGER"
     gene            1328..2251
                     /locus_tag="VGB85_17290"
     CDS             1328..2251
                     /locus_tag="VGB85_17290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135845.1"
                     /translation="MLAPALSLLLAIVAPGPAPAAPTSTPTPTPPPTVAEPEASPVPA
                     PPAEVAPAPAPEPAVIHAPITSVAPPPPVATPEPVRTPPPEFGPFFVAEPVSETAFPN
                     GPRTTDPPRFSLGGGSFCFVDDARCKSALILTADIGIGVNIVGGTRVPDLPYAQFTFR
                     GGLVFKPLTIGRKDAWHPWGIGLLGSWSRGTGAPSRESAVVSPHTDTWRVLLVNQLWL
                     SKRRNGFHLDLDLGIIRSTVLGGDKQFTGSSAGVSANWGGWGGIFLSGDFLDHDTRVV
                     FGFRGHGIATAPAIGLILLGLLAGGALGGGQ"
     gene            2248..3228
                     /locus_tag="VGB85_17295"
     CDS             2248..3228
                     /locus_tag="VGB85_17295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135846.1"
                     /translation="MSLLATSLLLLAITQQPSTPSAGPAVQRPTAPATTPAPVRAPAT
                     APAPATVPATAPATIVAPSVDVAAQPPVPPVPQDTPVVATATPPEVGPPFYTPADMQA
                     LRQRHGLEADPPTIERKPKFRCWVADPTCGFVIELNATSAYAMRVRQGSVNQANDFAR
                     WNSARVQYDVWFSIPTMVETRGKFRYTRLSLGPKGGVVASDNKDIWGNVGIAGRYWFS
                     RKRFAPALEFTSALVFKVAGINRAGNFDNVRGPMGFTADIGFGIGGFGSLIVGGQFDS
                     PLAREDVPDVERVSAAGMFFLGFRGNIVWGAPAAIAVGTHAAANRSVRSP"
     gene            3337..4005
                     /locus_tag="VGB85_17300"
     CDS             3337..4005
                     /locus_tag="VGB85_17300"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY0135847.1"
                     /translation="MVLPGSHLRPVPSDGTPDRSGPAANASTASAAEQTLELVQRAQA
                     GQMSAWSKLYQDNFERIYRHVRALTGDPHVSEELVQETFVQALLKISSFDRRSTFSTW
                     LHGVAINVVRNHWRSQKSTAKAHAKLEVVRSMDAPGDEPGMSVHRQQRVKALYAALET
                     LPDPLRVAFVLRDLEGLSPEEAAHRLEITPGNLAVRATRARQRIRKQLVAWGVLPGDA
                     EASP"
BASE COUNT          590 a         1652 c         1355 g          578 t
ORIGIN      
        1 gcgcaacctc tcggccggcg aggtccagct gctggccttc gcccgcgtcg ccgcccgcgc
       61 cccgaccctg ctgatcctcg acgaggcgac cgccagcgtc gacagcatga cggagcacaa
      121 ggtgcaggcc gcgatcgaag agctgctgcg gggtcgcagc gtcctggtca tcgcgcaccg
      181 tctcagcacc gtgcgccgcg ccgacaagat cctcgtcatg caggccggtg aggtcgtcga
      241 gcagggcacc cacgaggccc tgctcgcgct cggcggcgtg tacgccgagc tgtatcgcat
      301 gggcttcaag gagcccgagc cagccgcctg agcggtccga ggtccgggca cgcgaacgcg
      361 cggccgatcg atctcgttgc gtatacgagc atccggtgga cagcgttcgg gtcgcgcagc
      421 cctcggacgc gcagcccgaa gtcgcgcgtc gcgggccatg atcggccatg ctcgaccgaa
      481 cgggcgcgcg ttgtgagtac atcgagcgcc tgtgatcccc gatcggtgta cttgcgcgca
      541 taccggatca cgccgcgaca atgccggttg accggaccca gccgtcccat acaatccctg
      601 ccatgggcgt gcggcgggca aagaatgggg tcggcgaggt ggctgagcgc atctggggcg
      661 agtgcacggc gtaccgtctc cgcgtcatca accgcgctgt cagcaggatc tacgacgatg
      721 cgttacgacc ttaccgtctg cgcatcgccc agctcaacac gctcgtcgtc gtgatgcaga
      781 ccggcggcct gacgcccaac gacctgtcgc agcgcatgca catggacgcc tcgaccgtca
      841 gccgcaacgt cgagcgcatg tgcaagagcg gctggctcga gctcgccgac atcgacgacg
      901 cccgctcgca cgagatccgc atcaccgcca gaggtatgaa gctcatcgcc gacgtcgcgc
      961 cggcctggga gtccgcgcag cagcaggccg ccgagctgct cggcgtccag gtcagcaagg
     1021 ccatcgcgcg tggcggggac aagctcgtcg ccgacggcga gcgctgagcg tccggccggc
     1081 ggagatcccg gcggcccggg ctcctcgcgg gaatgtctga cggcgcgccg gagtaccccc
     1141 cccggtgccc gccccggtcc cgcaaaacaa cgacccgcgg gcgccacggc cagcgtgacg
     1201 gccagtgtga cggccatcgg ggccgcggag ggcgcgcccg aagtccgtcc gggtccgtcc
     1261 caggtccggc ggaggtccga cgcgggtggt tgtcacggcg cccggacttc tgcatgctgg
     1321 ggactccgtg ctcgcgcctg ccctgtcgct cctgctcgcg atcgtcgcgc ctggacccgc
     1381 cccggccgcg ccgacgtcca cgccgacgcc cacgcccccc ccgacagtgg ccgagcccga
     1441 ggccagcccg gtccccgcgc cgccggccga ggtcgcgccg gcccccgcac cggagccggc
     1501 cgtcatccac gcaccgatca cgtcggtcgc tccgccgccg cccgtggcga cgcccgagcc
     1561 ggtccgcacg ccgccgcccg agttcggccc cttcttcgtc gccgagcccg tctcggagac
     1621 cgccttcccc aacggcccgc gcacgaccga tcctccgcgc ttctcgctcg gcggcgggtc
     1681 gttttgcttc gtcgacgacg cgcgctgcaa gtcggcgctg atcctgaccg ccgacatcgg
     1741 catcggcgtc aacatcgtcg gcggcacgcg ggtccccgac ttgccctacg cgcagttcac
     1801 cttccgcggc ggcctcgtgt tcaagccgct gacgatcggc cgcaaggacg cctggcaccc
     1861 gtggggcatc ggcctgctcg gctcctggtc gcgcggcacc ggggccccct cgcgcgagtc
     1921 cgccgtcgtc tcgccgcaca ccgacacctg gcgcgtgctg ctcgtcaatc agctgtggct
     1981 gtcgaagcgc cgcaacggct ttcacctcga cctcgacctc ggcatcatcc gctccaccgt
     2041 gctcggcggc gacaaacagt tcacgggcag cagcgccggg gtctcggcca actggggcgg
     2101 ctggggcggc atcttcctca gcggtgactt cctcgaccac gacacgcgcg tcgtgttcgg
     2161 ctttcgcggc cacggcatcg ccacggcccc cgcgatcggc ctgatcttgc tcggcctgct
     2221 cgccggcggc gcgctgggag gtggccagtg agcctgcttg cgacgagcct cctgctgctg
     2281 gcgatcacgc agcagccctc caccccgagc gctggcccgg ccgtgcaacg cccgaccgcg
     2341 ccggccacca cacccgcgcc cgtgcgtgcc cccgcgactg cgcccgcgcc cgcgacggtg
     2401 cccgcgactg cgcccgcgac gatcgtcgcc ccgtcggtcg acgtcgccgc ccagccgccg
     2461 gtcccacctg tccctcagga cacgcccgtc gtcgccaccg cgaccccgcc cgaggtcggc
     2521 ccgccgttct acacgccggc ggacatgcag gcgctgcgtc agcgccacgg cctcgaggcc
     2581 gacccgccga cgatcgagcg caagccgaag tttcgctgct gggtcgccga cccgacctgc
     2641 ggcttcgtca tcgagctcaa cgccaccagc gcgtacgcga tgcgcgtccg ccagggcagc
     2701 gtcaaccagg ccaacgactt cgcgcgctgg aactccgccc gcgtgcaata cgacgtgtgg
     2761 ttcagcatcc cgacgatggt cgagacgcgc ggcaagttcc gctacacgcg gctgagcctc
     2821 gggcccaagg gtggcgtcgt cgccagcgac aacaaggaca tctggggcaa cgtcggcatc
     2881 gccggccgtt actggttctc gcgcaagcgc ttcgcgccgg cgctcgagtt caccagcgcg
     2941 ctggtattca aggtcgcggg catcaaccgc gccggcaact tcgacaacgt gcgcgggccg
     3001 atgggcttca ccgccgacat cggcttcggc atcggcggct tcggatctct catcgtcggc
     3061 gggcagttcg acagcccgct ggcccgcgag gacgtccccg acgtcgagcg cgtctcggcg
     3121 gccggcatgt tcttcctcgg cttccgcggc aacatcgtct ggggcgcccc ggccgcgatc
     3181 gccgtcggca cccacgcggc cgccaatcgc tccgtgcgct cgccctgagc gttcccgagc
     3241 gcccgatggc ccgacgtaca ccgtcccgcg ggcgatctcc tgagatctgc gggaccgctg
     3301 taatctgacc ctcgctcgcg ttgcatacga gggtccatgg tcctgcccgg ctcgcatctt
     3361 cgacccgtcc ccagcgacgg cacgcccgac cgctccggcc ccgccgcgaa cgcgtcgacc
     3421 gccagcgccg ccgagcagac cctcgagctg gtccagcgcg cccaggccgg ccagatgagc
     3481 gcctggtcca agctctacca ggacaacttc gagcgcatct atcgccacgt ccgcgccctg
     3541 accggcgacc cgcacgtcag cgaggagctg gtccaggaga ccttcgtgca ggccctcctg
     3601 aagatctcca gcttcgaccg ccgctcgacc tttagcacct ggctgcatgg cgtcgcgatc
     3661 aacgtcgtgc gcaaccactg gcgctcgcag aagagcaccg ccaaggcgca cgcgaagctc
     3721 gaggtcgtgc gctcgatgga cgcccccggc gacgagcccg gcatgagcgt gcaccgccag
     3781 cagcgcgtca aggccctcta cgccgcgctc gagaccctgc ccgatcctct gcgcgtcgcc
     3841 ttcgtgctgc gcgacctcga gggcctgagc cccgaggagg ccgcgcaccg cctcgagatc
     3901 acgccgggca acctcgccgt ccgggcgacc cgcgcgcgcc agcggatccg caagcagctg
     3961 gtcgcgtggg gcgtgctccc cggcgacgcg gaggccagcc catgacctcc cacgacctgt
     4021 cccatgaacc agacgagatc gacaccctcg cccgcgacgc ccgcgacgag ctggccgacg
     4081 cgatcacccg cgagctgcga gacatcgccc ccgacttcgc cgccgtcgtc gaggccgccc
     4141 acgagcgcga cccgctgcgc atcagccagg ccatg
//
LOCUS       DASWNJ010001024         2398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_230225, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001024 DASWNJ010000000
VERSION     DASWNJ010001024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230225"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2182
                     /locus_tag="VGB85_17305"
     CDS             <1..2182
                     /locus_tag="VGB85_17305"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HEY0135848.1"
                     /translation="PPEGDVARRARRRASPLVLVGCLLVSAAANYGGLWASLALYNSG
                     SSERPDPVVLRKVCVTGETRSVRLVDAALSGDPAQLMDGLRQVIEDGECEAQPVVVAL
                     VEEPRAERDPIEDLLAPEPEPREQPKKPKEKQKQKDREKPPEPEPEKEPEPEKPKPEP
                     EPEPEKPQEKIDFELQQLKMVEQMEDKDEKEAPSDAHYLSNINRDVTEETRSEISNLH
                     KDADKAKASQTEPSKDPEKGHADENKIAELKEQKSQLAQQAPKVKVSPVEQRPEQNDP
                     KPKTLLSMRDLPKREHSEEMAKNEAQASQDAVGELAPDRAAKSAVNPQPRNQSGATPT
                     KDQTLKFRLSKNDLDALFGRDVAAQKAIISQRESKKKGIWEEQRERYQSPLENMIPEV
                     RPGNQTALRSRKHPFAQFIATMHRTIHDKWAYNFLEQLDTMGRSHALNKVELWTRVEI
                     VLLGDGTIDKVRTVRFSGNMQFDNAAREVVFNSAPYPTPPKEILSPNGKVYIHWAFHR
                     DERACGTFGAEPFILDNAGNGERPDPNRAVRPASSESEALASRRLSRQLGPATPGPVG
                     PAPPPPPAGDGHNHPPGEGHEHEAENENEGAGGDGPGDGTVADPAARTSADSWLKSFA
                     AGDAAKLASRSSLPFYVGEQIVARTREELTGVLSSMLEEAGGKPGASTVYTAGGLRKR
                     FGSVPSGVMEGLGRNYAVTKLGGEVFILLLEKHFGSWKVVGVAR"
BASE COUNT          471 a          829 c          833 g          265 t
ORIGIN      
        1 cccgcccgag ggggacgtgg cccgcagggc ccggcgacgc gccagcccgc tggtgctggt
       61 cggctgtctg ctggtctccg cggcggccaa ctacggcggt ctgtgggcca gcctggcgct
      121 ctacaacagc ggcagcagcg agcggccgga cccggtcgtg ctgcgcaagg tgtgcgtcac
      181 cggcgagacc cgcagcgtcc gcctcgtcga cgccgcgctg tcgggcgacc cggcgcagct
      241 gatggacggg ctgcgacagg tgatcgagga cggcgagtgc gaggcgcagc cggtcgtcgt
      301 cgcgctggtc gaggagccgc gcgccgagcg tgacccgatc gaagacctgc tggcgccgga
      361 gcccgagccg cgcgagcagc cgaagaagcc gaaagagaag caaaagcaga aggacaggga
      421 gaagccgccc gagcccgagc ccgagaagga gcccgagccc gagaagccca agcccgagcc
      481 cgagcccgag cccgagaagc cgcaggagaa gatcgacttc gagctgcagc agctcaaaat
      541 ggtcgagcag atggaggaca aggacgagaa ggaggcgccg agcgacgcgc actacctctc
      601 gaacatcaac cgcgacgtca ccgaggagac ccgctcggag atctcgaacc tccataaaga
      661 cgccgacaag gccaaggcct cgcagaccga gccgagcaag gaccccgaga agggccacgc
      721 ggacgagaac aagatcgccg agctcaagga gcagaagagt cagctcgcgc agcaggcccc
      781 gaaggtcaag gtcagcccgg tcgagcagcg cccggagcag aacgacccca agcccaagac
      841 gctgctgtcg atgcgcgacc tgcccaagcg cgagcactcg gaggagatgg ccaagaacga
      901 ggcgcaggcg agccaggacg cggtcggcga gctggcaccg gaccgcgccg cgaagtcggc
      961 tgtcaacccg cagccgagga accagagcgg tgcgacgccg accaaggatc aaacgctcaa
     1021 gttccggctg tcgaagaacg acctcgacgc gctgttcggc cgcgacgtcg ccgcgcagaa
     1081 ggcgatcatc agccagcgcg agtcgaagaa gaagggcatc tgggaggagc agcgcgagcg
     1141 ctaccagtca ccgctcgaga acatgatccc cgaggttcgc cccggcaacc agacggccct
     1201 gaggtctcgc aagcacccgt tcgcgcagtt catcgcgacg atgcaccgaa cgatccacga
     1261 caagtgggcc tacaacttcc tcgagcagct cgacacgatg ggccgcagtc acgcgctcaa
     1321 caaggtggag ctgtggaccc gcgtcgagat cgtgctgctc ggtgacggca cgatcgacaa
     1381 ggtccgcacg gtccgcttct ccggcaacat gcaattcgac aacgcggccc gcgaggtcgt
     1441 gttcaactcg gccccctacc cgaccccgcc caaggagatc ctcagcccca acggcaaggt
     1501 ctacatccac tgggcgttcc atcgcgacga gcgcgcctgc ggcacgttcg gcgccgaacc
     1561 gttcatcctc gacaacgccg gcaacggcga gcgccccgac cccaaccgcg cggtgcgtcc
     1621 ggcgagctcc gagtccgaag cgctggcgag ccgaagattg tcacgccagc tcggcccggc
     1681 gaccccggga ccggtgggcc ccgccccacc cccaccgccc gccggcgacg gccataacca
     1741 cccgcccggc gagggccacg agcacgaggc cgagaacgag aacgagggtg caggcggcga
     1801 cggcccgggc gacggcacgg tcgccgaccc ggccgcgcgt acttcggccg actcgtggct
     1861 caagtcgttc gcggccggcg acgcggcgaa gctcgcgagc cgctcgagcc tgcccttcta
     1921 cgtcggcgag cagatcgtcg cccgcacgcg cgaggagctg accggcgtgc tcagctcgat
     1981 gctcgaggag gccggtggca agcccggcgc gtcgacggtg tacacggccg gcggcctgcg
     2041 caaacgcttc ggcagcgtgc ccagcggggt gatggaaggg ctcggccgca actacgcggt
     2101 caccaagctc ggcggcgagg tgtttattct gttgctcgag aagcacttcg gatcgtggaa
     2161 ggtcgtcggc gtcgctcgct gagctgcgcg gacaggacat cgcagcggcc ggtcctgtat
     2221 catgctggcc atggccgccc ccaacccgtc cgcgcaaggg ctccggatcg accgtttgca
     2281 ggtcgagaag ttccgctcgc tgcgacacgt ggcctggcca gaggacggga tgggctggaa
     2341 cggtacggtc ccggagatcg tcctggtcgg cggcgcgaac ggcagcggca agacgacg
//
LOCUS       DASWNJ010001025         1950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_689116, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001025 DASWNJ010000000
VERSION     DASWNJ010001025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689116"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1771
                     /locus_tag="VGB85_17310"
     CDS             <1..1771
                     /locus_tag="VGB85_17310"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEY0135849.1"
                     /translation="TLAIERAATGETLVLDTRDLEIEAVEAATMPLTTPPRLATSDLA
                     WATTTWQLGSLHPVLGRPLTIALPPGVNVVRVRYRTSPDSTGLQWLAPEQTADHAAPF
                     LYTQSQAIHARSWAPVQDSPGVRITYDARITTPAPTRPVMAAEALAPETGPEGQVHHR
                     FALDQSIPPYLLALAVGRLESASTGPRTGVWAEPSLIKRASAEFADMEAMLTATERQF
                     GPYRWGRYEVLVLPPAFPFGGMENPRVTFVTPTILAGDRSLVALIAHELAHSWSGNLV
                     SNATWRDLWLNEGFTVYIERRILEAVYGAERAEIEAVLGRQHLEGELAAMADRPGDQV
                     LHVELAGRDPDDVFSDVPYEKGYLLLRRIEEAYGRPAFDAFVQRWFSEHAFTSRTTEE
                     FKTFVRAALLAPQPPLLSQTVPDIELWLSSPGLLPDAPQPSSAALQKVAADAIAVAHG
                     ELAAKQLDSRGYSPMHWIYFLRALPLAEVDAVRVLGQVDAAFHFTDSGNNEVLGEWLI
                     LTSAHDYRAADARLERFLLTVGRRKYLTPIYDALLRTAAGQQLARSIYPRARPGYHAI
                     TRRTLDALLDWKPSSEPTPAADP"
BASE COUNT          271 a          782 c          630 g          267 t
ORIGIN      
        1 caccctggcg atcgagcgcg ccgcgaccgg cgagaccctc gtgctcgaca cccgcgatct
       61 cgagatcgag gccgtcgagg ctgccaccat gcctctgacg acgccgcctc gcctcgcgac
      121 cagcgacctc gcctgggcga cgacgacctg gcaactcggc agcctgcacc ccgtgctcgg
      181 ccgtccgctg acgatcgcgt tgccccccgg cgtcaacgtc gtccgcgtcc gctaccgcac
      241 cagccccgac tcgaccggcc tgcagtggct cgcgccggag cagaccgccg accacgccgc
      301 gccgtttttg tacacgcagt ctcaggcgat ccacgcccgc tcgtgggcgc cggtgcagga
      361 cagccccggc gtgcggatca cctacgacgc ccggatcacc acgcccgcgc cgacccgccc
      421 ggtgatggcc gccgaggccc tggccccgga gacaggtccc gagggccagg ttcaccaccg
      481 cttcgcgctc gaccagtcga tcccgccgta cctgctcgcg ctcgcggtcg gccgcctcga
      541 gtcggcgagc accggcccgc gcacgggcgt gtgggccgag ccgagtttga tcaaacgcgc
      601 cagcgccgag ttcgccgaca tggaggcgat gctcaccgcc accgagcgcc agttcgggcc
      661 gtatcgctgg ggtcgctacg aggtgctcgt gctgccgccc gcgttcccct tcggcggcat
      721 ggagaaccca cgcgtcacct tcgtgacgcc gacgatcctc gccggcgatc gctcgctcgt
      781 cgccctgatc gcccacgagc tggcccacag ctggtccggc aacctggtct cgaacgcgac
      841 ctggcgcgac ctgtggctca acgagggctt caccgtgtac atcgagcggc ggatcctcga
      901 ggccgtctac ggggccgagc gcgccgagat cgaggccgtg ctcgggcgcc agcacctcga
      961 gggcgagctc gccgcgatgg ccgaccgccc cggtgaccag gtgctgcacg tcgagctcgc
     1021 cggccgcgac cccgacgacg tgttctccga cgtcccctat gaaaagggct acctgctgct
     1081 gcgccgcatc gaggaggcct acggccgccc ggccttcgac gcttttgtgc agcggtggtt
     1141 ctccgagcac gcgttcacca gccgtaccac cgaggagttc aagaccttcg tgcgcgcggc
     1201 cctgctggcc ccgcagccgc cgctgctcag tcagaccgtc cccgacatcg agctgtggct
     1261 gagctcgccc ggcctgctcc ccgacgcgcc gcagccgagc agcgccgccc tgcaaaaggt
     1321 cgccgccgac gcgatcgccg tcgcccacgg cgagctcgcg gcgaagcagc tcgacagccg
     1381 cggctacagc ccgatgcact ggatctactt cctgcgcgcg ctgccgctgg ccgaggtcga
     1441 cgcggtgcgc gtgctcggcc aggtcgacgc cgcgtttcac ttcaccgact cgggcaacaa
     1501 cgaggtgctc ggcgagtggc tgatcctgac ctccgcgcac gactaccggg ccgccgacgc
     1561 ccgcctcgag cgcttcctgc tgacggtcgg ccgccgcaag tacctcacgc cgatctacga
     1621 cgccctgctg cgcaccgcgg cgggccagca gctcgcgcga tcgatctacc cgcgcgcccg
     1681 gcccggctat cacgcgatca cgcgacgcac cctcgacgcg ctgctcgact ggaagccgtc
     1741 gagcgagccg accccggccg cggacccctg acgacggaac cccgcggcac gcgacacccc
     1801 gtcacgcaag cgggcgggtc agccggctga cttcgcgggc ttgccgggcg ccgccgccag
     1861 cggctggccg aacttacgcg cgcaggtcga ctctagcgtc tcgacgcggt cgcgcccgat
     1921 ctgcaccgag gccgtcgaca gcgcgttttg
//
LOCUS       DASWNJ010001026         2185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_477472, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001026 DASWNJ010000000
VERSION     DASWNJ010001026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477472"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="VGB85_17315"
     CDS             complement(<1..983)
                     /locus_tag="VGB85_17315"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0135850.1"
                     /translation="MSQALETGGASDELATRHEDGHEPAPELEQQPFRIGRFTILRRL
                     GRGGMGVVYAAYDDRLDRKIALKLVYASPQDGSLGQTRLLREAQALARLSHPNVVQIY
                     EVGEHDRQVFLAMEFVSGVTLRGWSRAGAHGWREILAVYLQAGRGLAAAHAAGLVHRD
                     FKPDNAVLADDGRVRVLDFGLARALLDESDDSPAHSNCLAQLDSQDLTNPLTAAGTLL
                     GTPAYMSPEQLLRHPTDARSDQYSFCVALWEALYGARPFESRTLGELKQRVFSGPPRL
                     PRVRTSVPAHLGRVLLRGLAIHPDRRYPDMDALLRDLADDGRVRVLDFGLAR"
     gene            complement(1089..1970)
                     /locus_tag="VGB85_17320"
     CDS             complement(1089..1970)
                     /locus_tag="VGB85_17320"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEY0135851.1"
                     /translation="MLRKVLRYLVLPLAVIVAVVFGQAYVRNVELRADLPPVAGGGGG
                     ASVLIVLSAAQTLTLREGGAHPTGFFLNELSEPLAHLLAAGYAVEFASPGGRPPRVDA
                     HSVEFLVGDARVQALALIAAQPLDSPHALEDLSEAHLERFIGVFVPGGHAPMQDLHAD
                     PALGRVLAHFHSSGKPTAALCHGPAALLSAPIVDGQWTYAGYRMTGFPRYTERALELF
                     GVIDGHVPFYLDEALTDAGAVYTTTFPPIIPRVIRDRELVTGQDPYAAERLGAKFVHM
                     LRLYQQLGGNVDWSAPA"
     gene            complement(1960..>2185)
                     /locus_tag="VGB85_17325"
     CDS             complement(1960..>2185)
                     /locus_tag="VGB85_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135852.1"
                     /translation="LALGVYSLLAALHRDDEVRRLAIRCFLVFHFAVVTVQAVGFAQG
                     GPLQPVIAHGLLLLAFVCVRVSQGGRGHAA"
BASE COUNT          336 a          720 c          849 g          280 t
ORIGIN      
        1 cgcgcgaggc cgaagtcgag gacgcgcacg cggccgtcgt cggcgaggtc gcgcagcagc
       61 gcgtccatgt cggggtagcg ccggtcgggg tggatcgcca ggccgcgcag gagcacgcgg
      121 ccgaggtgcg ccggcacgct ggtgcgcacc cggggcaggc gcggcgggcc gctgaacacg
      181 cgctgcttga gctcgccgag ggtgcggctc tcgaacggac gcgcgccgta cagcgcctcc
      241 cacagcgcga cgcagaagct gtactggtcg gagcgcgcgt cggtcgggtg acgcagcagc
      301 tgctcggggg acatgtacgc gggcgtgccg agcagggtgc cggcggcggt cagcgggttg
      361 gtgaggtcct gcgagtcgag ctgcgcgagg cagttgctgt gggcgggcga gtcgtcggac
      421 tcgtcgagca atgcgcgcgc gaggccgaag tcgaggacgc gcacgcggcc gtcgtcggcg
      481 agcacggcgt tgtccggctt gaagtcgcgg tgcacgaggc cggcggcgtg ggcggcggcg
      541 aggccacgtc cggcctggag atatacggcg aggatctccc gccagccgtg tgcgccggcg
      601 cgcgaccagc cgcgcagggt gacgccgctg acgaactcca tggcgaggaa gacctggcgg
      661 tcgtgctcgc cgacctcgta gatctggacg acgttggggt gcgagaggcg agcgagcgcc
      721 tgggcctcgc gcagcagccg ggtctgcccg agcgatccgt cctgcggcga ggcgtagacc
      781 agcttgaggg cgatcttgcg gtcgaggcgg tcgtcgtagg cggcgtacac gacgcccatg
      841 ccgccgcgcc cgagccggcg caggatcgtg aagcggccga tgcgaaacgg ctgctgctcg
      901 agctccggcg cgggttcgtg accgtcttcg tggcgggtcg ccagctcgtc gctggccccg
      961 ccggtctcga gcgcttgcga catcagtcgc cggcagtcta gcgggcgccc gtgggcgatg
     1021 acagcgcgcg cgccccccac cgcgcacggc cgccgtgcgc ggagtcgggg cgtggtggcc
     1081 ccggtcgatc aggcgggggc gctccagtcc acgttgccgc cgagctgctg gtaaagtcgc
     1141 agcatatgta cgaacttcgc gccgagccgc tcggctgcgt aggggtcctg gccggtgacc
     1201 aattcgcggt ctctgatcac cctggggatg atgggcggga aggtggtggt atacacggcg
     1261 ccagcatcgg tgagcgcctc gtcgaggtag aacgggacgt ggccgtcgat cacgccgaac
     1321 agctcgagcg cgcgctcggt gtagcgcggg aatccagtca tgcggtaccc ggcgtaggtc
     1381 cattgaccat cgacgatcgg cgccgacagc agcgcagccg gcccgtggca caacgcggcc
     1441 gtcggcttgc ccgacgagtg aaagtgcgcg agcacccggc ccagcgcggg gtccgcgtgc
     1501 aggtcttgca tcggcgcatg accgccgggg acgaacacgc cgatgaaccg ctccaagtgc
     1561 gcttcggaca ggtcttcgag ggcgtgcggc gagtccagcg gctgggcggc gatcaacgcg
     1621 agcgcctgga cacgggcgtc tccgacgagg aactcgacgc tatgcgcgtc gacccgtggc
     1681 ggacggccgc ccgggctcgc gaactcgacg gcgtagcccg ctgcgagcag gtgcgcgagc
     1741 ggctcgctga gctcgttgag gaagaagccc gtcgggtggg cgccgccctc acgcaacgtc
     1801 aaggtttgcg cagcggacag cacgatcagc acggacgcgc cgccaccacc cccggcgacc
     1861 ggggggaggt cggcgcgaag ctcgacgttg cgtacgtacg cctggccgaa cacgaccgcg
     1921 acgatcaccg cgagcgggag gacgaggtaa cggagcacct tacgcagcat gtccacgccc
     1981 tccctgggag acgcgcacgc acacgaacgc cagcagcagc agtccatggg cgatcaccgg
     2041 ctgcaacggc cctccctggg cgaagccgac cgcttgtacc gtgaccacgg cgaagtggaa
     2101 caccaggaag cagcggatcg ccaggcggcg gacctcgtcg tcgcggtgga gcgcggcaag
     2161 taggctgtac accccgagcg cgagg
//
LOCUS       DASWNJ010001027         2758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_477541, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001027 DASWNJ010000000
VERSION     DASWNJ010001027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477541"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1772)
                     /locus_tag="VGB85_17330"
     CDS             complement(<1..1772)
                     /locus_tag="VGB85_17330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0135853.1"
                     /translation="MRDETNVPAAGSQASPASPSSPSHGTGPGPSLSRLIDLASECMA
                     VLDLEGRVVLVNAAWRQALGSEVVAGAPVEGLLRPEDREAWREAWARLGPDARLSVTA
                     RVRGGDAPLLWSLHRSDAELVVYAAGRAAPVEAQLQRSAVHLEVLRSVVANVPLVLFA
                     VDTRGQIVLLEGRGLAAVGLEPHIFDPRADARTPGKSAFDVFAGIPSLLDGVRRALAG
                     AALRGVFEFLDRSFETYTAPFSDSRGHLQGVIGVASDVTESVRSDRRLRDLNEKLEAA
                     RDEAIEASRAKSAFLANMSHELRTPLNAIIGYSEIAQDELRSHAAREADGRGPLAQLG
                     NDVQKIHVAGTHLLGIISDILDLSKIEAGRMELHIETFSVQNMIDDVVATAQRLVAQN
                     GNVFEVRAAPELGEMTADLTKVRQILFNLLSNSAKFTERGAVTLEVGKAAAESGEGEQ
                     VFEFCVSDSGIGMSQEQKDRLFQSFYQGDSSTTRKYGGTGLGLAISRRFAQMMGGEIT
                     VQSSLGSGSKFFVRLPAVIRGHLGVEETTAPGRSAAPADVVAAPQAMLVIDDDPNVYD
                     MMERILSREGLDVYGAATGKEGLAL"
     gene            complement(1842..2696)
                     /locus_tag="VGB85_17335"
     CDS             complement(1842..2696)
                     /locus_tag="VGB85_17335"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY0135854.1"
                     /translation="MSVPARPRLALAVSLLLGGCYVGFDGALEAGSTGTTTGGATAGS
                     VTTNDATGSDASTDPTVDPSTDPGSETSGAVSGDTSTGEIGATTGGDEPMTTDPATSD
                     PSATTTGASGDETTGGADSGMMPPPVDVPDNAHCADVADWSPAWAQLEQDVLVIVNQV
                     RSKGANCGTKGAFNPAGPLTMNPALRCAARKHSADMAARNFFDHVNPDGEKFTDRMAK
                     AGYGGYSTAGENIAAGSPDAQGTMSQWMGSDGHCANIMNPDYKDIGVGYSTGGQYGHL
                     WTQVFGAK"
BASE COUNT          363 a         1037 c          938 g          420 t
ORIGIN      
        1 agcgcgaggc cctccttgcc ggtcgccgcg ccgtacacgt cgaggccctc gcggctgagg
       61 atccgctcca tcatgtcgta gacgttcggg tcgtcgtcga tgacgagcat cgcctgcggc
      121 gcggcgacga cgtcggccgg cgcggcgcta cggcccggcg ccgtcgtctc ctcgacgccg
      181 aggtgcccgc ggatcaccgc cggcaggcgc acgaagaact tcgagccgga cccgaggctg
      241 ctctgcaccg tgatctcgcc gcccatcatc tgcgcgaagc ggcggctgat cgccaggccg
      301 aggccggtgc cgccgtactt gcgggtcgtc gacgagtcgc cctgataaaa gctctggaac
      361 aggcggtcct tctgctcctg cgacatcccg atcccgctgt cgctgacgca gaactcgaac
      421 acctgctcgc cctcgcccga ctccgcggcg gccttgccga cctcgagggt caccgcgccg
      481 cgctcggtga acttggccga gttactgagc aggttgaaca ggatctggcg gaccttggtc
      541 aggtcggccg tcatctcgcc gagctccggc gccgcgcgga cctcgaagac gttgccgttc
      601 tgggcgacga gccgctgcgc ggtggcgacg acgtcgtcga tcatgttctg caccgagaag
      661 gtctcgatgt gcagctccat gcggccggcc tcgatcttcg acaggtcgag gatgtcgctg
      721 atgatcccga gcaggtgcgt gccggcgacg tggatcttct gcacgtcgtt gccgagctgg
      781 gccagcggac cgcggccgtc ggcctcgcgc gccgcgtgac tgcgcagctc gtcctgggcg
      841 atctccgagt agccgatgat cgcgttgagc ggcgtgcgca gctcgtggct catgttggcc
      901 aggaaggcgc tcttggcccg gctcgcctcg atcgcctcgt cgcgggcggc ctccagcttc
      961 tcgttgaggt cgcgcaggcg gcggtccgat cgcacgctct cggtgacatc cgaggcgacg
     1021 ccgatcaccc cctgcaggtg cccgcgactg tcgctaaacg gcgccgtgta ggtctcgaag
     1081 ctgcggtcga ggaactcgaa caccccccgc agcgccgcgc cggcgagcgc ccgccgcacg
     1141 ccgtcgagca gcgacgggat gcccgcgaac acgtcgaagg ccgacttgcc gggcgtgcgc
     1201 gcgtcggccc gcggatcgaa gatgtgcggc tcgaggccga cggccgccag cccacgaccc
     1261 tcgagcaaga cgatctgccc gcgcgtgtcg accgcgaaca gcaccagcgg cacgttcgcc
     1321 accacgctgc gcagcacctc gaggtgcacc gcgctgcgct gcagctgggc ctcgaccggc
     1381 gccgcgcggc cggccgcgta caccaccagc tcggcgtcgc tgcggtgcag gctccacagc
     1441 agcggcgcgt cgccgccacg cacccgcgcc gtcacgctca gccgcgcgtc gggcccgagc
     1501 cgggcccacg cctcgcgcca cgcctcgcgg tcctccggtc gcagcaggcc ctcgaccggc
     1561 gcgcccgcga cgacctcgct gccgagcgcc tgacgccacg cggcgttgac gagcacgacc
     1621 cggccctcga ggtcgagcac cgccatgcac tcgctcgcga ggtcgatcag gcgcgacaga
     1681 ctcggccctg gaccggtccc atgggacggg gaggacgggg aggccggtga ggcctgcgaa
     1741 cccgcggccg gtacattcgt ctcgtcacgc atggcgcccg cggctcgccg agggtgcccc
     1801 atcctagcgg ctggcgcgac gaaatcaacg ccgccgacgg gctacttcgc cccgaagacc
     1861 tgtgtccaca ggtgtccgta ctgtccgccg gtcgagtagc cgacgccgat gtccttgtag
     1921 tccgggttca tgatgttggc gcagtggcca tcgctgccca tccactggct catcgtcccc
     1981 tgcgcgtcgg gcgagccggc cgcgatgttc tctcccgcgg tgctgtagcc cccgtagccg
     2041 gccttggcca tgcggtccgt gaacttctcg ccgtcggggt tgacgtggtc gaagaagttg
     2101 cgcgccgcca tgtcggccga gtgcttccga gccgcgcagc gcagcgccgg gttcatcgtc
     2161 agcgggccgg ccggattgaa cgcccccttg gtcccgcaat tggccccctt gctgcgcacc
     2221 tgattgacga tgacgaggac gtcctgctcg agctgcgccc acgcgggcga ccagtcggcg
     2281 acatcggcgc aatgggcgtt gtcggggacg tccacgggcg ggggcatcat ccccgagtcg
     2341 gcgccgccgg tcgtctcatc gccgctcgcg ccggtcgtcg tcgccgatgg atcgctcgtg
     2401 gccggatccg tcgtcatcgg ctcgtctcca cccgtggtcg caccgatctc gcccgtgctc
     2461 gtatccccgc tcaccgcgcc gctcgtctcg ctgcccggat cggtcgacgg gtccaccgtg
     2521 ggatccgtgc tcgcgtcgct gcccgtcgcg tcgttcgtcg tcaccgatcc cgccgtcgcg
     2581 ccgcccgtcg tcgtgcccgt gctgcccgcc tcgagcgcac cgtcgaagcc gacgtagcag
     2641 ccgccgagca gcaacgagac ggcgagcgcg aggcgtggtc gagccggaac ggacatgcga
     2701 gcacgatagc cccgagcccc gcccgggctc aagcgcccgc gaccgacacg cgcgaccg
//
LOCUS       DASWNJ010001028         1980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_401179, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001028 DASWNJ010000000
VERSION     DASWNJ010001028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401179"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VGB85_17340"
     CDS             complement(<1..261)
                     /locus_tag="VGB85_17340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135855.1"
                     /translation="MGSKLGLVGVGRLCGIALAFAGGCGDAGGGTEGPGSSSGVVSTG
                     GGSTTDVSTTTAPTTDPTTEPAPTSTGTGDLSTTTVEPGETLD"
     gene            834..1541
                     /locus_tag="VGB85_17345"
     CDS             834..1541
                     /locus_tag="VGB85_17345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135856.1"
                     /translation="MRPSLALLAVLAVSCACESATTQADLAPIEAPVERRDLDRGMIL
                     TRPATPLSANPVVELPEEPAPEEAKPDDDLTSLAALEAAVRERLPEHRLACARVKRLP
                     CRMLGDLDGDGTRDIVALVEPEGSRALGLAVLWAHGGVDLLGAGRRGQKWLMQRDGTA
                     EREPIPADLSTVGRWDVWAADGPEGARRGFVDRKRHVRAPAVRGDGILLEGGDLAPTV
                     AYHDGKGWRLQYLEPLN"
     gene            1671..>1980
                     /locus_tag="VGB85_17350"
     CDS             1671..>1980
                     /locus_tag="VGB85_17350"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0135857.1"
                     /translation="MRRLREMVVVITGASSGLGRATAYQLAEHGCRLVLAARRVDELE
                     KTAEGCRERGSEALVVETDVTSEDAVNRLVAAAIDRWGRIDLWINNAGVTLFARIEEG
                     A"
BASE COUNT          280 a          711 c          709 g          280 t
ORIGIN      
        1 gtcgagggtc tcgccgggct cgaccgtcgt ggtcgagagg tcgccggtgc ccgtgctcgt
       61 cggcgcgggc tcggtggtcg gatccgtggt cggggcggtg gtcgtcgaca cgtcggtcgt
      121 cgacccaccg cccgtcgaga ccacgccgga gctgctcccg gggccctccg tgccgccccc
      181 cgcgtcaccg cacccgccgg cgaaggccag cgcgatacca cacaaacgac ccaccccaac
      241 caaccccaac ttgcttccca tgtgacacct gtacaccacg gccacgatgc acacaacggc
      301 cgcgagcgcc gccggatcgg ccttgtccgc gcgatttgga cgaggcagtc gctcgatgtg
      361 gacggcgttt tttggacctg agctcggcga atacagcctc gataaattgc aatgtgcgat
      421 cgatcggacg atctcggata tgtcgatgac agcgccgagc attcgccgat gtacataaaa
      481 ccacggagag cgcgtgggtc gacgtcgaag cccgggcagt gcgcggtgcg accggccggc
      541 ggcgaggtgg tgcgccctgc gctcctgcga cgcatgactg cgcggcgctt gcaggctcgt
      601 cgtggcagcg aggtcgcgtc gggcccgacg cccgcgtcgg gcccgggtcc gcgaccgtcg
      661 cagcccgcgc agcggggagc ctcgccgcgg atcaccgtta cagcgggcct cgcacgtgag
      721 aaggatcccg aaccgacgca ggatgcgtgg tgtcggccac tgcgaggctc cgagagtccg
      781 tcgtcgtgac gttctcttgc ttcgtcggcg ccgcactgtg gcacgatccc cgcgtgcgtc
      841 cctccctcgc gctcctcgct gtcctcgccg tgtcctgtgc ctgtgagtcg gccacgacgc
      901 aggccgacct cgcgccgatc gaagcgccgg tcgagcgccg tgacctcgac cgcggcatga
      961 tcctgacgcg accggcgacg ccgctgtccg cgaaccccgt ggtcgagctc cccgaggagc
     1021 ccgcgcccga ggaggccaag cccgacgacg acctcacctc gctcgcggcc ctcgaggccg
     1081 ccgtgcgcga gcgactgccc gagcatcgcc tcgcgtgcgc gcgggtcaag cggctgccgt
     1141 gccgaatgct cggtgacctc gacggcgacg gcaccaggga catcgtcgcg ctcgtcgagc
     1201 cggagggcag ccgcgcgctc gggctcgccg tgttgtgggc gcacggcggc gtcgacctgc
     1261 tcggtgccgg ccgtcgcggc cagaagtggt tgatgcagcg agacggcacc gccgagcgcg
     1321 agccgatccc cgccgacctc tcgacggtcg ggcgctggga tgtatgggcg gcggacgggc
     1381 ccgagggggc gcgtcgcggc ttcgtcgacc gcaagcgcca cgtccgggcc cccgcggtgc
     1441 gtggggacgg catcttgctc gaaggcggcg acctggcgcc gacggtcgcc tatcacgacg
     1501 gcaagggctg gcgcctgcaa tacctcgagc ccttgaatta aagggcgcag gcgacgcagg
     1561 cgctcgcctg tggggccgcg gggcggtgat acgcggtgat acgcgccgca gccatcccga
     1621 caccacgcgc ccgctggcgc cgcggcgatc cacgtccaca ctgcgcggac atgcgacgcc
     1681 tgcgcgagat ggtagtcgtg atcaccggcg catcgagcgg cctcggtcgc gcgaccgcgt
     1741 atcagctcgc cgaacacggc tgccggctcg tgctcgcggc ccggcgcgtc gacgagctcg
     1801 agaagacggc cgagggctgt cgcgagcgcg gcagcgaggc cttggtggtc gagaccgacg
     1861 tcacctcgga ggacgcggtg aaccgcctcg tcgccgcggc gatcgaccgc tggggccgca
     1921 ttgatctctg gatcaacaac gcgggcgtca ccttgttcgc acggatcgag gagggcgcgc
//
LOCUS       DASWNJ010001029         6550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_331028, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001029 DASWNJ010000000
VERSION     DASWNJ010001029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6550
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331028"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1569)
                     /locus_tag="VGB85_17355"
     CDS             complement(<1..1569)
                     /locus_tag="VGB85_17355"
                     /inference="COORDINATES: protein motif:HMM:NF020075.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEY0135858.1"
                     /translation="MLRLPTVCLLAALPAAALCFVESARAAPVLAGDPQEADPGLVAS
                     GEYRSEPLRQAQRKHGGAPGDLAQPDAPPTAVTPAPLPSAFCQIKVNGVPKDTETDYL
                     PHVIQCENGGANLEALKAQAIAARSVAYYAMAENGTICDGQGCQVYSCGSQPSAIHIQ
                     AVQETAGQYLSFNNTEKGRVLTYAFFVSGDNDQTANCHGVSLNANTENYVTYNEGKTW
                     TGVKETSLGFQYKTPSAYGYGQNRGCMSQWSARCLENNKGYKYDAIIRYFYGEDIEIA
                     QAQGPCVGKVDFAAKYAAQSFPLASQPAVVLTQGEVYDAWIDLTNVGDQTWTANTKLA
                     PTPRDVASPLAGGMWLSPTRITGPDADTPPDAVGRFSFQIAANEPGDYFQTFGLVEEG
                     VTWFADGPKGGGPPDDQLEIHVTVVPAPPDPSTGTGTEDTGVDVTGSDATGPDVTSAT
                     ASDGSGEGTSGSASGSASASGTGDATGDTPTTGGVGSSGDEGGSSGGPMGEDDDSGCG
                     CRGTDSAPMGLLTPL"
     gene            complement(1754..2791)
                     /locus_tag="VGB85_17360"
     CDS             complement(1754..2791)
                     /locus_tag="VGB85_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135859.1"
                     /translation="MHPRSAALGLCLSLAAACGDDGRREGASAGTATAGSITLTISGG
                     DASGTGTGGTPTTGEGGSGEGGTGSSTVDPSGDQPPKFDLGAAPDVGMQEQPEACAKV
                     DLLFVIDNSSSMKNEQDNLVASFPGFVSSMLAQLANADSYHVGVVSTDSYDHNDDPCD
                     NVLGGLITRTGGNDSSNQSCGPYASGKRYMSEADDLGARFTCAAQVGTDGATDEKPLE
                     AAIRGLGPILNAPGECNDGFLRKDALLVLVLITDEEEEGSNGDPPDWYDSLVLIKGGI
                     ETNIVVLSLIGLENPPCVKAAEVGHRIIEFTEKFTYGSVGQICSDSYNLFFSDAIAVI
                     SEACNNFVPPA"
     gene            3061..6519
                     /locus_tag="VGB85_17365"
     CDS             3061..6519
                     /locus_tag="VGB85_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0135860.1"
                     /translation="MRSFGPALLTLFASVQARAQAPGVAPVTSEPVTIEGSVKDENTD
                     DPVPQALVILQCSCLQGQREVMTNADGLYRFRDLPPGRYTAQALAGQANVNKSFTATG
                     GIFRVSFVVDPANRFVIHHTIENVVRRDAVSRTKIKPEQLRLAGTGGTNRDFTSVVDV
                     APTVTSDAGGLRLGGTTSAETRYTLDNANITTPAFGTLSSTIVQEFIEEVEVLEAGYD
                     AEYGGASGGQVRARRVGGSNKLRGVVLMRAAPRLAAPRLIAATDESLRVAEIAEVDAQ
                     GVFQLSGPIVRDRVFFAVGVAPGGQRNSMIQSFYRRRDKDRSGGYESCAYENGTADCA
                     SNGNYIDSVKFAEQRFRTGRFDLQWSATLDWQITPKHRLRLSGGSSPISFRRTSFRLP
                     PGSEPSAFGTNPTQTLGGQSRVGQGVINDTFGSKYASATGVGLDYEGRVDNDRIEIDA
                     TLYYSKFRSTDAWRLDDAGLKNVPLLQQTDTQGRNLFDLLDRDGATRLVPGVDTACNN
                     TELPGLTCPTRTWLSGGLGNYSNYTQDRGGGLFALTHFLNTRRAGAHQIKYGVEIDTV
                     RFRQSTAYSGSNSADFYSNCPAGQQGGGEYCYDPATRSYAVAAGMGRVDNHRSVTVDG
                     DNPDIRTSIGYGRVRAEQNDLRAIASPIGAGVRVPAYDATLATQNYGVFLQDRWAILS
                     NLYVNAGARWELQDMKDLLGRRNVFIRDNVAPRLGLSYDWTDEGKSRLYASYGWFFSQ
                     LPVLLNSRAFGGLVNVTRTYRASDCRTPTSGGEPRSDAAGIPTEHCLDSNQSTTGLSA
                     GAVVPRLRGMFTKQFQVGYEQEVVEDLVIGVNWLHQDLGRAVEDVSTNGGLNYLVANP
                     GESVSASDIARQEQACRELTDRLAAAAVDDPGRDMLSRELNHCKFLAEAFDRVGNLFP
                     RPTRNYDAWTLKINKRFARSWILQASYTYSRLIGNYDGFITRNTGAINLGASPQYDLP
                     DLVRNSFGPLFDNRPHLAKVDTFYTFDLRNAGRLTLGASVRFQSGSPISLYADNIRYR
                     GQFVTYLLPRGAGGRLEPNYFGNISVSFAYPLPRELELEITARLANITNARAVLRVDE
                     VYSFSTTRPIAGGDFEDLKHAKVLSPNAPTSFFRRDIVPRQGNFGVQTVFQQPIQAQF
                     EVRLRF"
BASE COUNT         1018 a         2356 c         2149 g         1027 t
ORIGIN      
        1 cagcggcgtc agcagcccca tgggcgcgct gtcggtcccg cggcagccgc agccggagtc
       61 gtcgtcctcg cccatcgggc cgcccgacga gccaccctcg tccccgctcg acccgacgcc
      121 gccggtggtc ggcgtgtcgc cggtcgcgtc gccggtcccg ctcgcgctcg cgctgccgct
      181 cgcgctgccg ctggtgccct cgccgctgcc atcactggcc gtcgcgctcg tgacatcggg
      241 gccggtcgca tcggagccgg tgacatcgac gccggtgtcc tcggtgccgg tgcccgtcga
      301 cgggtcaggc ggcgcgggca cgaccgtcac gtggatctcg agctggtcgt cgggcggtcc
      361 gccgcccttg ggaccgtcgg cgaaccaggt cacgccctcc tcgacgaggc caaaggtctg
      421 gaagtagtcg ccgggctcgt tggcggcgat ctggaacgag aaccgaccga ccgcgtcggg
      481 cggcgtgtcg gcgtcgggcc cggtgatccg cgtcggcgac agccacatac cgcccgccag
      541 cggcgaggcg acgtcgcgcg gcgtcggggc gagcttggtg ttcgccgtcc aggtctggtc
      601 gccgacgttg gtcaggtcga tccacgcgtc gtagacctcc ccctgcgtca acaccaccgc
      661 cggctgcgag gccagcggga acgactgcgc ggcgtacttg gccgcgaagt cgaccttgcc
      721 gacgcacggg ccctgcgcct gcgcgatctc gatgtcctcg ccgtagaagt aacggatgat
      781 cgcgtcgtac ttgtaaccct tgttgttctc gaggcagcgc gccgaccact gcgacatgca
      841 gccgcggttc tggccgtagc cgtaggccga gggggtcttg tactggaagc cgagcgaggt
      901 ctccttgacg ccggtccagg tcttaccctc gttgtaggtc acataatttt cggtgttggc
      961 gttgaggctc acaccatggc aattcgcggt ctggtcattg tcgccggaca cgaagaatgc
     1021 ataggtgagc acccggccct tttcggtgtt gttgaacgac aggtactggc ccgccgtctc
     1081 ctgcaccgcc tggatgtgga tcgccgacgg ctgcgacccg cacgaataga cctgacagcc
     1141 ctggccgtcg cagatcgtgc cgttttcggc catcgcgtag taagcgaccg agcgcgccgc
     1201 gatcgcctgg gccttgagcg cctcgaggtt cgccccgccg ttttcgcact ggatcacgtg
     1261 cggcaggtag tcggtctcgg tgtccttggg caccccgttg accttgatct ggcaaaacgc
     1321 cgacggcagc ggggccggcg tgacggccgt cggcggcgcg tcgggctgcg cgaggtcgcc
     1381 gggcgcacct ccgtgcttgc gctgcgcctg gcgcagcggc tcgctgcggt actcgccgct
     1441 ggcgaccagc cccgggtcgg cctcctgggg gtcaccggcg aggaccggcg ccgcgcgggc
     1501 agactcgacg aaacagagcg ccgcagcggg cagcgcggcg aggagacaga cggtggggag
     1561 acgaagcatg gggatccagg tcgagtgtcc actgtacccg gttcacgcgg cgcggtggcc
     1621 gacatgacgc acagccctgg cttcgcagac cccgccgggc gcagggccaa cggggggcga
     1681 tggaccacgc gcccgcgctc gcgggcctgt tcaccccacg accaccccac gaccacccca
     1741 cgaccacacc ggctcaggcc ggcgggacga agttgttgca ggcctcgctg atcaccgcga
     1801 tcgcgtcgct gaagaacagg ttgtacgagt cggagcagat ctggccgacg ctcccgtagg
     1861 tgaacttctc ggtgaactcg atgatgcgat ggccgacctc ggccgccttc acgcacggcg
     1921 ggttctccag gccgatcagc gacagcacga cgatgttggt ctcgatgccg cccttgatca
     1981 acaccagcga gtcgtaccag tcgggcgggt cgccgttgga cccctcctcc tcctcgtcgg
     2041 tgatcagcac cagcaccagc agcgcgtcct tgcgcagaaa gccgtcgttg cactcgcccg
     2101 gcgcgttcaa gatcgggccg agcccgcgga tcgccgcctc gagcggcttc tcgtcggtcg
     2161 cgccatccgt accgacctgc gccgcgcagg tgaagcgcgc cccgaggtcg tccgcctcgc
     2221 tcatgtagcg cttgccgctc gcgtatggcc cgcagctctg gttgctcgag tcgttgccgc
     2281 cggtgcgcgt gatcagcccg ccgagcacgt tgtcgcaggg gtcgtcgttg tgatcgtagc
     2341 tgtccgtgct gacgacgccg acgtgataac tgtcggcgtt ggcgagctgc gccagcatcg
     2401 agctgacgaa gcccgggaag ctggccacga ggttgtcctg ctcgttcttc atactgctcg
     2461 agttgtcgat cacgaacagc aggtcgacct tggcgcaagc ctccggctgc tcctgcatgc
     2521 cgacatccgg ggccgcgccg aggtcgaact tcggcggctg atcgccggag ggatcgacgg
     2581 tcgagctgcc agtcccgccc tcgccgctgc caccctcgcc ggtcgtcggc gtgccgccgg
     2641 tgccggtgcc gctcgcgtcc ccgcccgaga tcgtcagggt gatcgatccg gccgtcgcgg
     2701 tgcccgcgga ggcgccttca cggcgaccgt cgtccccgca ggcggcggcg agagataaac
     2761 agaggcccag ggctgcgctg cgggggtgca tcgcagcgaa ggctaggcca gaccggcccg
     2821 cagatacaag cgccctgacg ttcaggtatc accgggacag cactgcggac acgcgccccc
     2881 gcagggggat acactgcccc acggcgaaac caccgccgcc ggggtcgggt ccgcttgctg
     2941 tcccgagaga catttcccgc gcaagtccga agatcgtccg gtgccccgcc cccgcaagct
     3001 tgccaggctt cgatcgttcg ccgcgtatgg gacgcggccg cgtgcgatcg ggggtgggtg
     3061 atgcgatcgt tcggtccggc gcttctgaca ctgttcgcca gcgtacaggc gcgcgcccag
     3121 gcccccggcg tggcgcccgt cacctccgag cccgtgacga tcgagggatc cgtcaaggac
     3181 gagaataccg atgacccggt cccgcaggcg ctggtgatcc tccagtgctc gtgtctgcag
     3241 ggccagcgcg aggtgatgac caacgcggac ggcctgtatc gcttccgcga cctgccgcca
     3301 ggcaggtaca cggcccaggc gctcgccggc caggccaacg tcaacaagtc gttcacggcc
     3361 accgggggca tatttcgggt gtccttcgtc gtcgaccccg ccaaccgctt cgtcattcat
     3421 cacacgatcg agaacgtcgt gcgtcgggac gcggtcagcc gcacgaagat caagccggag
     3481 cagctgcgcc tcgccggcac cggcggcacc aaccgcgact tcacctcggt ggtcgacgtc
     3541 gcgccgaccg tcacctccga cgccggcggc ctgcgcctgg gcggcacgac gagcgcggag
     3601 acccgctaca ccctcgacaa cgccaacatc acgacgccgg ccttcgggac cctgagctcg
     3661 acgatcgtcc aggagttcat cgaggaggtc gaggtgctcg aggccggcta tgacgccgag
     3721 tacggcggcg cgagcggcgg gcaggtgcgg gcgcggcggg tcggggggtc caacaagctg
     3781 cgcggggtcg tactgatgcg cgcggccccg cggctggccg cgccgcggct aatcgccgcg
     3841 accgacgagt cgctgcgcgt cgccgagatc gccgaggtcg acgcgcaggg cgtgttccag
     3901 ctgtccgggc cgatcgtgcg cgaccgggtg ttcttcgcgg tcggcgtcgc cccgggcggg
     3961 cagcgcaact cgatgatcca gtcgttttat cgccgccgcg acaaggatcg ctccggtggt
     4021 tacgagtcgt gcgcctacga gaacggcacc gccgactgcg cgtcgaacgg caactacatc
     4081 gattcggtga agttcgccga gcagcggttt cgcaccggcc gcttcgacct gcaatggtcg
     4141 gcgaccctcg actggcagat cacgcccaaa catcgcctgc ggctgtcggg cggcagcagc
     4201 ccgatctcgt tccggcgcac cagctttcgc ctgccgccag gctccgagcc gagcgccttc
     4261 ggcaccaacc cgacgcagac cctcggcggt cagtcgcgcg tcggccaggg cgtcatcaac
     4321 gacaccttcg gcagcaagta cgcgtccgcg accggcgtcg gcctcgacta cgagggccgc
     4381 gtcgacaatg acaggatcga gatcgacgcg acgctctact acagcaagtt tcgcagcacc
     4441 gacgcctggc gcctcgacga cgccggcctc aagaacgtgc cgctgctgca acagaccgac
     4501 acgcagggcc gtaacctctt cgacctgctc gaccgcgacg gcgcgacccg cctggtgccc
     4561 ggggtcgaca cggcctgcaa caacaccgag ctgccgggcc tgacctgccc gacgcgcacg
     4621 tggctgtcgg gcgggctcgg caactactcg aattacacgc aggaccgcgg cggaggcctg
     4681 ttcgcgctga cccacttttt gaatacgcgc cgcgccggcg cgcaccagat caagtacggc
     4741 gtcgagatcg acacggtccg gtttcgtcag tcgacggcgt actcgggcag caacagcgcc
     4801 gatttttata gcaactgtcc ggccggccag cagggcggcg gcgaatattg ctacgacccg
     4861 gcgacccgca gctacgcggt cgccgccggg atgggccgcg tcgataatca ccgcagcgtc
     4921 acggtcgacg gcgacaaccc cgacatccgc acgagcatcg gctacggccg cgtgcgcgcc
     4981 gagcagaacg acctgcgcgc gatcgcctcg ccgatcggcg ccggcgtgcg cgtgccggcc
     5041 tacgacgcga ccctcgccac ccagaactac ggggtcttcc tgcaggaccg ctgggcgatc
     5101 ctctcgaact tgtatgtcaa cgccggcgcc cgctgggagc tgcaggacat gaaggacctg
     5161 ctcggccgcc gtaacgtgtt catccgcgac aacgtcgccc cacgcctcgg cctcagctac
     5221 gactggaccg acgagggcaa gagccgcctc tacgccagct acgggtggtt cttcagccag
     5281 ctgcccgtgc tgctcaacag ccgcgccttc ggcggcctcg tcaacgtcac ccgcacgtat
     5341 cgtgcctcgg actgccgcac gccgacctcc ggcggcgagc cgcgcagcga cgccgccggc
     5401 atccccaccg aacattgcct cgacagtaat cagagcacca ccggcctcag cgccggcgcg
     5461 gtggtcccgc gcctgcgtgg catgttcacc aaacagttcc aggtcggcta cgagcaggag
     5521 gtcgtcgagg acctcgtcat cggcgtcaac tggctgcatc aggacctcgg ccgcgcggtc
     5581 gaggacgtct ctacaaatgg cggcctcaac tacctcgtcg ccaatcccgg tgaatcggtg
     5641 tccgccagcg acatcgcccg ccaggagcag gcctgccgcg agctgacgga tcgcctcgcc
     5701 gcggcggcgg tcgacgaccc ggggcgcgac atgctgagcc gcgagctgaa ccactgcaaa
     5761 ttcctcgcgg aggccttcga ccgcgtcggc aacctgttcc cgcgcccgac ccgcaactac
     5821 gacgcgtgga ctttaaaaat caacaaacgc ttcgcccgca gctggatctt gcaggcgagc
     5881 tacacctatt cgcggctgat cggaaattat gatggattta tcacccgcaa caccggcgcg
     5941 atcaacctcg gcgcgagccc gcagtatgac ctgccggacc tggtgcgcaa cagcttcggc
     6001 ccgctgttcg acaaccgccc gcacctcgcc aaggtcgata cgttttacac cttcgatctg
     6061 cgcaacgccg gccgcctgac gctcggcgcg tcggtccgct tccagtcagg cagcccgatc
     6121 agtctttatg cggacaacat ccgctaccgc ggccagttcg tcacatactt gctcccgcgc
     6181 ggcgccggcg gccgcctcga gccgaattat tttggaaata tcagcgtcag cttcgcctat
     6241 ccgctgcccc gcgagctcga gctcgagatc accgctcgcc tcgccaatat caccaacgcg
     6301 cgcgcggtgc tgcgcgtcga cgaggtctac tcgtttagca cgacgcgacc gatcgccggc
     6361 ggcgacttcg aggacctcaa acacgccaag gtcctcagcc ccaacgcgcc gaccagcttc
     6421 tttcgccgcg acatcgtccc ccgccagggc aacttcggcg tgcagaccgt gttccagcag
     6481 ccgatccagg cgcaattcga ggtgcggctg cgattctagg ggcggatgtc cggccggggg
     6541 cgcactctcc
//
LOCUS       DASWNJ010001030         3081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_158534, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001030 DASWNJ010000000
VERSION     DASWNJ010001030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158534"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="VGB85_17370"
     CDS             <1..779
                     /locus_tag="VGB85_17370"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0135861.1"
                     /translation="VWEYPSIDLDDALDKHIPVAYSSRFQQMLGYAEEELPQVADSWW
                     RAIHPDDVALSIRAISDHLAGRQKPLFVQYRVYTRQGDLRWWQIYGGTLPDASPRRVR
                     AIGVARDITDMKRAEETLRDQLDLITRQQSAIRAMAAPIIQVWDGIITLPLIGNLDRD
                     RAHEIMDRLLSEIVRRRSRHAILDLTGIEIVDTDTAEHLYRIVGAVRLLGARAVLSGL
                     QPSVAQILTELGADVAPFITYRTLQDALQACVRGDARPRG"
     gene            complement(776..1870)
                     /locus_tag="VGB85_17375"
     CDS             complement(776..1870)
                     /locus_tag="VGB85_17375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135862.1"
                     /translation="MRRGTEDDLDAPLRRALSLATRAASPGNTQPWRFRRDGDSLEVH
                     CDDDSEPSTLNHAGRVSAMTLGGLLELLAIALGAEGYQAIIRLPEQSTPGGPWAVVEL
                     RPVDLSVDPLVHAIAGRFTDRREYRGGDLGDPVFDRIQGELAGHPGCGLRWLPRAAVP
                     EPLMRRIVDAELFFWRHAPLHRQIMALTRWSRTHAARTRDGVPWQAQGIPAVAATVLR
                     FCDRWPVQAALNRLGMLARVERTTRSLVTSAAGLGLISVRSMSRAALVAASRLHARAS
                     LHLHAAGYATQPFTPVSLYASEAELDVLPADWSADDRRRFAEGMPVLREAFGLGRDEL
                     PLWLFRVGVSPGPREVGSYTHRYELDRVLL"
     gene            complement(1867..>3081)
                     /locus_tag="VGB85_17380"
     CDS             complement(1867..>3081)
                     /locus_tag="VGB85_17380"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HEY0135863.1"
                     /translation="EDVLLALGQLWCAGVDVDWDAYYAGERRRRVPLPTYVFERVHHE
                     HPVAAGRRGVVPGEPPRVKQAPAPEPTRAPAAASDPALQVLRELFASMLGVESARPDD
                     HFFDLGGSSFMALRMRVQIEERLGVKLPVHTFVESPTLAGLADRVRSALAPAEGPVEA
                     ATRRLLVPLRSGRGGPELVMIQPIGGTVFTYRALARHLDPAYTITGVRASGMEPGEPV
                     LATVEAIAARYLEELADRGAPPVLCGHSAGGVLAFEMARQLRARGGQAPLVVLLDTPP
                     LSVARGLTLTSDEDTLQVAEMMRSEGSEAHAEFARALREDASFREIVRVTWQALAVHE
                     PAPADLDVLYVQARASGPEGGDRAWMELCRGAFTRVSVDADHFTMLDEPTVATLARYI
                     ERAFEAGARSRR"
BASE COUNT          464 a         1193 c         1039 g          385 t
ORIGIN      
        1 gcgtctggga atatccgtcg atcgacctcg acgacgcgct cgacaaacac atacccgtcg
       61 cctattcgtc gcggttccag cagatgctcg gctacgccga ggaggagctc ccccaggtcg
      121 ccgacagctg gtggcgcgcc atccaccccg atgacgtcgc gctgtcgatc cgcgcgatct
      181 ccgatcacct cgccggccgg caaaagccac tattcgtcca gtatcgcgtg tacacgcggc
      241 aaggcgacct gcgctggtgg cagatctacg gtggcaccct gccggacgcc tcgccccggc
      301 gcgtgcgcgc gatcggggtc gcacgcgaca tcacggacat gaagcgcgcc gaggagacgc
      361 tgcgcgacca gctcgacctg atcacccgcc aacaatccgc gatccgcgcg atggctgcgc
      421 cgatcattca ggtgtgggac ggcatcatca cgctgccgct gatcggcaac ctcgaccgcg
      481 accgcgccca cgagatcatg gaccgcctgc tctcggagat cgttcgccga cggtcccgcc
      541 acgcgatcct cgacctcacc ggcatcgaga tcgtcgacac ggacacggcg gagcacctgt
      601 accgcatcgt cggcgcggtc cgcctgctcg gcgcccgcgc ggtgctgagc ggcctgcagc
      661 cgtcggtcgc ccagatactg accgagctcg gcgccgacgt ggccccgttc atcacctacc
      721 gcacgctgca ggacgcgctg caggcgtgcg tccgcggcga cgcgcggccg aggggctaaa
      781 gcagcacccg gtcgagctcg taccggtggg tgtagctgcc gacctcgcgc ggcccggggc
      841 tcacgccgac tcgaaacagc cacagcggca gctcgtcgcg ccccaggccg aaggcttcgc
      901 gcagcacggg catgccctcc gcgaagcggc gccggtcatc cgcgctccag tcggccggca
      961 gaacgtcgag ctcggcctcg ctcgcgtaca gagacaccgg ggtgaacggc tgagtcgcgt
     1021 aaccggccgc gtgcaggtgc aacgacgcgc gcgcgtgcaa gcgactggcg gcgaccagcg
     1081 ccgcgcgaga catcgatcgt acggagatga gaccgagccc cgcggcgctg gttaccagcg
     1141 accgcgtcgt gcgctcgacc cgggcgagca tgcccagtcg attgagcgcg gcctggaccg
     1201 gccagcggtc gcagaaccgg agcaccgtcg cggcgaccgc ggggatgccc tgcgcctgcc
     1261 acggcacgcc gtcgcgggtc cgcgccgcgt gcgtccgaga ccagcgggtc agcgccatga
     1321 tctgacggtg cagcggcgcg tgccgccaga agaacagctc ggcgtcgacg atgcggcgca
     1381 tcagcggctc cggcaccgcc gcccgcggca gccagcgcag gccgcagccc gggtggcccg
     1441 ccagttcgcc ctgtatccga tcgaacactg gatcgccgag atctccgccg cgatactcgc
     1501 ggcgatcggt gaagcggccc gcgatcgcgt gcaccagcgg gtcgaccgac agatcgaccg
     1561 gccgcagctc gactaccgcc cagggtccgc ccggcgtcga ctgctcgggc aatcggatga
     1621 tcgcctggta cccctccgcg ccgagagcga tcgccaacag ctcgagcagg ccgccgagag
     1681 tcatcgcgct gacccgaccg gcgtggttga gcgtgctcgg ctcggagtcg tcgtcgcaat
     1741 ggacctccag cgagtcgccg tcccggcgaa accgccacgg ctgggtgttg cccgggctcg
     1801 cggcgcgggt cgccaggctc agcgcgcgcc tgagcggcgc atccagatcg tcctccgtcc
     1861 ctcgtctcat ctgcggctcc tcgctccggc ctcgaacgcc cgctcgatgt accgcgcgag
     1921 cgtggcgacc gtcggctcgt ccagcatcgt aaagtggtcc gcgtcgacgc tcacgcgcgt
     1981 gaaggcgccg cggcacagct ccatccacgc ccggtcgccg ccctccggcc cggacgcccg
     2041 cgcctggacg tacagcacgt ccagatcggc cggcgccggc tcgtggaccg ccagcgcctg
     2101 ccacgtgacc cggacgatct cgcggaagct ggcgtcctct cgcagcgccc gcgcgaactc
     2161 ggcgtgcgcc tcggagccct cgctgcgcat catctcggcg acctgaagcg tgtcctcgtc
     2221 gctcgtcagc gtcagcccgc gcgcgaccga cagcggcggc gtgtcgagca acaccacgag
     2281 cggcgcctgg ccgccgcggg cgcgcagctg acgggccatc tcgaaggcca acacgccgcc
     2341 cgcggagtga ccgcagagca ccggcggcgc gccgcgatcc gcgagctcct cgaggtagcg
     2401 cgccgcgatc gcctccacgg tcgccagcac cggctcgccg ggctccatcc cggacgctcg
     2461 aacgccggtg atcgtgtacg cgggatcgag gtggcgcgcg agcgcgcggt aggtgaacac
     2521 cgtcccgccg atcggctgga tcatcaccag ctccgggccg ccgcgcccgg atctcagcgg
     2581 cacgagcagc cgccgcgtcg cagcctccac cggcccctcc gcgggcgcga gcgccgaacg
     2641 aacgcggtcg gcgaggccgg cgagcgtggg cgactcgacg aacgtgtgca ccggcagctt
     2701 gaccccgagc ctctcttcga tctgaacccg catccgcagc gccatgaacg acgatccgcc
     2761 gaggtcgaaa aaatgatcgt cgggccgcgc cgactcgacg ccgagcatcg acgcgaacag
     2821 ctcgcgcagc acctgcagcg ccgggtcgct cgccgccgcg ggcgcgcgcg tcggctccgg
     2881 cgcgggcgcc tgcttcaccc gcggcggctc gcccgggacc accccgcggc gacccgcggc
     2941 gaccggatgc tcgtggtgca cccgctcgaa cacgtaggtc ggcagcggca cccggcggcg
     3001 gcgctcgccg gcgtaatacg cgtcccaatc gacgtccacc cccgcgcacc acagctggcc
     3061 cagcgccagg agcacgtcct c
//
LOCUS       DASWNJ010001031         4046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_88834, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001031 DASWNJ010000000
VERSION     DASWNJ010001031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88834"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3313
                     /locus_tag="VGB85_17385"
     CDS             <1..3313
                     /locus_tag="VGB85_17385"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0135864.1"
                     /translation="PLVRPNLPPEAEEALCGYVPAAVVIRLAAGQRGWMAELRRVAVI
                     FIHLPTVNYATPLGEAQQAMVAVQQVMQSAEGSVTHVSVQPDGATLMAAFGLPPLAHE
                     DDATRAVEAAQQLRAVLGRLGFPARMGVTTGKAFCGSVGGAARCEYTVLGDVVNLAAR
                     LMQAADEQRPILCDGPTYNAARAHFTFDTYPPITVKGKAQPIVIHGPAGNRLVQKGGK
                     ISTLRPPTSDGPGTPRPALVGRMRERVVLIGKLHELLRGRSATVVIEGETGLGKSRLA
                     DDLLEQAGAMGVRRLIGAREAADAAAPYHAWTPVFRAIFGLDDTPGADADPWDRVTTA
                     LRTLVRGAAARAPLLAPALALDAAETDSTAAIAGKARAEMTRDLLTAILRSDLDDSPA
                     LLLIEDAHWVDSASWALLAQVREQVGPLLCVLVTGPIESDTDPAPSELRQVLADPATH
                     HLLLGPLSLAETETLVCRKLGVSRAPQEVIEFVYGRAEGHPLFTEELTWALRDAGLLS
                     ILGNECYLAPEAGDLKSAHFPDNLEAVITSRIDRLSPQLQLIVKVGSVLGRVFPLSLL
                     RAVHPIQADHAGLSEHLATLERRGLLILKGGSVADPTYGFKHALTQEVAYNLLPFALR
                     QQLHQQIAEHYERVFKDRIELHYPLVAHHWYSVVAPGALVSEDQRPAPALIARATEYL
                     QRAGDQAIRNGVGQEAVEHYSHALEVLPALPASSARTEQEIALQIGLGNALIAARGVA
                     SDEVERTFARARELCREVGETKHLFPVLFGLWTYYFVRAEHRVARELAEQILVFAERR
                     KERVPLLIAHRTLGTQMFHVGDLPRARAHLERAVALYAPDFDRPLAFLYRHDPRVASL
                     SLLAICLHMQGNHAEANERSTVALAVARELGHRASMSLASGHAAILRQVAGRVTAPKE
                     LSVLHPLGVEHSNAPWDSFVQVVDAWTLAEQGQLAEASDLLRDALVELQVSGTSVFWP
                     WVLGVYADLCARGGRQAEALGHLHEAIGASQQSGARVWEAELLRRRGELLRQGTTGDV
                     LGSHERTPEDCFVAAIHRAREQGARIFELRAAQSLDDLEGRSDAAVALIEETRMRLTG
                     KLDDPALAALGRPRA"
     gene            3412..>4046
                     /locus_tag="VGB85_17390"
     CDS             3412..>4046
                     /locus_tag="VGB85_17390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135865.1"
                     /translation="MRQQPLGDDRRERGLEAVREAARRAGGRCLSDTYVNHDTKLEFE
                     CRSGHRWHTRPRVVTAGKWCPECGHRPRIRLVAMQALAKVRGGACLSTSYVAAAPGMR
                     WRCAEGHEWSATPARITAGGWCPDCTGRWTIERLQALAAERGGRCLSRSMEEGTNDAC
                     MQWECARGHRFAVRKRCIIAGQWCPECHQHDVVWQRTLARAQALAANQGGR"
BASE COUNT          539 a         1429 c         1549 g          529 t
ORIGIN      
        1 gccgctggtg cgcccgaact tgccgccgga ggccgaggag gcgctgtgcg gctacgtgcc
       61 ggcggcggtg gtgatccggc tggcggccgg ccagcgcggg tggatggcgg agctgcggcg
      121 ggtcgcggtg atcttcatcc acctgccgac ggtcaactat gcgacgccgc tgggcgaggc
      181 gcagcaggcg atggtcgcgg tccagcaggt gatgcagagc gccgagggca gcgtcacgca
      241 cgtcagtgtg caaccggacg gggcgacgct gatggcggcc ttcggtctgc cgccgctcgc
      301 ccacgaggac gacgcgacgc gggcggtcga ggcggcgcag cagctgcgcg cggtgctcgg
      361 gcggctcggg tttccggcgc gcatgggcgt gacgaccggc aaggcgttct gcgggtcggt
      421 cggcggcgcg gcgcgctgcg agtacacggt gctcggggac gtcgtgaacc tggcggcgcg
      481 cctgatgcag gcggccgacg agcagcgccc gatcctgtgc gacggcccga cctacaacgc
      541 cgcgcgcgcg cacttcacct tcgacacgta tccgccgatc acggtcaagg gcaaggccca
      601 gccgatcgtg atccacgggc ccgcgggcaa tcgtctcgtg caaaaaggcg ggaagatctc
      661 gaccctgcgg ccgccgacca gcgacggtcc gggcacgccg aggccggcgc tcgtcggtcg
      721 catgcgcgag cgggtggtcc tgatcggcaa gctgcacgag cttctgcgcg ggcgcagcgc
      781 gacggtcgtc atcgagggcg agacgggtct cggcaagtcg cgactcgcgg atgacctgct
      841 cgagcaggcc ggggcgatgg gcgtgcgccg gctgatcggc gcgcgcgagg ccgccgacgc
      901 ggcggcgccg taccacgcgt ggacgccggt gttccgcgcg atcttcgggc tcgacgacac
      961 gcccggcgcc gacgcggatc cgtgggaccg ggtgacgacg gcgctgcgga ccctggtccg
     1021 cggcgcggct gctcgcgcgc cgctgctggc cccggcgctg gcgctcgacg ccgccgagac
     1081 cgacagcacc gcggcgatcg ccggcaaggc gcgcgccgag atgacccgcg atctactgac
     1141 cgcgatcctg cgcagtgacc tcgacgacag cccggcgctg ctgttgatcg aggacgcgca
     1201 ctgggtcgac tcggcctcgt gggcgctgct cgcccaggtg cgcgagcagg tcggaccgct
     1261 gctgtgcgtg ctggtcaccg ggccgatcga gagcgacacc gacccggcgc cgtccgagct
     1321 gcgccaggtg ctcgccgacc cggcgacgca ccacctgctg ctcgggccgc tgtcgctcgc
     1381 cgagaccgag actttggtgt gtcgcaagct cggggtctcg cgcgcgccgc aggaggtgat
     1441 cgagttcgtg tacgggcgcg ccgagggcca cccgctgttc acggaggagc tgacctgggc
     1501 gctgcgcgac gccggcctgc tgtcgatcct cggcaacgag tgctacctgg cccccgaggc
     1561 aggtgacctc aagtcagcgc acttccccga caacctcgag gcggtgatca cgagccgcat
     1621 cgaccgcctg tcgccgcagc tgcagttgat cgtcaaggtc ggcagcgtgc tcgggcgggt
     1681 gttcccgctg tcgctgctgc gcgcggtcca cccgatccag gccgaccacg ccgggctgtc
     1741 ggagcacctc gcgacgctcg agcgtcgcgg cctgctgatc ctcaaaggcg gcagcgtggc
     1801 cgacccgacc tacggcttca agcacgcgct gacgcaggag gtcgcctaca acctgctgcc
     1861 cttcgcgctg cgccagcagc tgcaccagca gatcgcggag cactacgagc gcgtgttcaa
     1921 ggaccgcatc gagctgcact acccgctggt cgcccaccac tggtacagcg tcgtcgcccc
     1981 gggcgcgctg gtctcggagg accagcgccc ggcgccggcg ctgatcgccc gggcgaccga
     2041 gtacctgcag cgcgcgggcg accaggcgat ccgcaacggc gtcggtcagg aggcggtcga
     2101 gcactacagc cacgcgctcg aggtgttgcc cgcgctgccg gccagctcgg cgcgcaccga
     2161 gcaggagatc gcgctgcaga tcgggctcgg caacgcgttg atcgccgcgc gcggggtcgc
     2221 gtcggacgag gtcgagcgga ccttcgcccg cgcccgcgag ctgtgccgcg aggtcggcga
     2281 gaccaagcac ctgttcccgg tgctgttcgg cctgtggacg tactacttcg tccgcgccga
     2341 gcaccgcgtc gcccgcgagc tggccgagca gatcctggtg ttcgcggagc gccgcaagga
     2401 gcgggtgccg ctgctgatcg cccaccgcac gctcgggacg cagatgttcc acgtcggcga
     2461 cctgccgcgc gcgcgggccc acctcgagcg agcggtcgcc ctgtacgcgc ccgacttcga
     2521 ccgcccgctg gcgttcctgt atcgccacga cccgcgggtc gcgagcctgt cgctgctggc
     2581 gatctgcctg catatgcaag gcaaccacgc cgaggccaac gagcgcagca ccgtcgcgct
     2641 cgcggtcgcc cgcgagctcg gccaccgcgc gagcatgtcg ctggcgagcg ggcacgccgc
     2701 gatcctccgg caggtcgcgg gcagggtcac cgcgccgaag gagctgtcgg tgttgcaccc
     2761 gctcggcgtc gagcacagca acgcgccgtg ggacagcttc gtgcaggtcg tcgacgcgtg
     2821 gacgctcgcg gagcagggac agctggccga ggcgagcgac ctgctgcggg acgcgctggt
     2881 cgagctgcag gtcagcggca ccagcgtatt ctggccgtgg gtgctcgggg tgtacgccga
     2941 cctgtgcgcg cgcgggggcc ggcaagccga ggcgcttggc cacctgcacg aggcgatcgg
     3001 cgcgtcgcag cagtcgggcg cgcgcgtgtg ggaggcggag ctgctgcgcc gccgcggcga
     3061 gctgctgcgc cagggcacca ccggcgacgt gctcggctcg cacgagcgca ccccggagga
     3121 ctgctttgtc gccgcgatcc accgcgcgcg cgagcagggc gcccgcatct tcgagctgcg
     3181 cgcggcgcag agcctcgacg acctcgaggg gcgatcggac gcggccgtcg ccctgatcga
     3241 ggagacccgc atgcgcctga ccggcaagct cgacgacccc gcgctcgccg ccctcggccg
     3301 cccgcgggcg tgaccgtgtt cggcggatcg gcccatgggg ggcctgggac gcgcgctgga
     3361 aagcctcgcg cggtattgag cgcgcccgcg cggaccggta gagtgtaggc catgcgtcag
     3421 caacccctgg gcgacgatcg ccgcgagcgc gggctggagg ccgtccgcga ggcggctcgc
     3481 cgggcgggcg ggcgctgcct ctcggacacc tacgtcaacc acgacaccaa gctcgagttc
     3541 gagtgccgca gcgggcatcg ctggcacacg cggccgcggg tcgtcaccgc ggggaagtgg
     3601 tgccccgagt gcggccaccg gccgaggatc cgcctggtgg cgatgcaggc gctggcgaag
     3661 gtgcgcggcg gcgcgtgcct gtcgacatcc tacgtcgcgg cggcgccggg catgcgctgg
     3721 cgctgcgccg agggtcacga gtggtcggcg acgccggcga ggataaccgc gggcgggtgg
     3781 tgtccggatt gcaccggccg ctggacgatc gagcgcttgc aggcgctcgc ggcggagcgg
     3841 ggcgggcggt gcctgtcgcg cagcatggag gaggggacca acgacgcgtg catgcaatgg
     3901 gagtgcgccc gcgggcatcg cttcgcggtc cgcaagcggt gcatcatcgc gggtcagtgg
     3961 tgccccgagt gtcaccagca cgacgtggtg tggcagcgga ccctcgcgcg cgcgcaggcc
     4021 ctcgcggcca atcagggcgg gcgctg
//
LOCUS       DASWNJ010001032         1691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_511472, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001032 DASWNJ010000000
VERSION     DASWNJ010001032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511472"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..630
                     /locus_tag="VGB85_17395"
     CDS             145..630
                     /locus_tag="VGB85_17395"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEY0135866.1"
                     /translation="MRAGRVVRVNVAAEGGVPKRPVVAALLTVHGVAGDRQRNLKYHG
                     GPDRAVCLYSQERIDALIAEGHPISAGSTGENLTIAGLAWEELRVGDRLAVGEVAVLE
                     LTEPTTPCRTIAGSFKKGEFKRISDVLHPGWSRFYARVVIEGLVREGAQVRRLDPDDA
                     P"
     gene            653..913
                     /locus_tag="VGB85_17400"
     CDS             653..913
                     /locus_tag="VGB85_17400"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEY0135867.1"
                     /translation="MTDKPLVARCEPTVLTLEPGIYWWCACGRSKTQPFCDGTHDITD
                     IKPVKLRIRETSEVALCNCKHSGNKPHCDGTHARMTASEPGT"
     gene            complement(976..>1691)
                     /locus_tag="VGB85_17405"
     CDS             complement(976..>1691)
                     /locus_tag="VGB85_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEY0135868.1"
                     /translation="GVEREVTVPRRDACEECDGTGAAAGTKPQACATCHGQGQVIHRQ
                     GFFTVQTACPRCHGEGQVIASPCRGCGGTGVVQKETTLKLSIPAGVDDGQTLRIPGRG
                     MAGTKGGPAGNLYVVVAVEADPRFQRDEFDIHSKVSISMAQAALGCTVKTPSLQGDQD
                     LEIEAGTQPGHVVVRKGKGIPVLGGRGHGDHHVHVEVTVPQKLSQEQEELLRELAESF
                     GEAVSQKKGFFSSLRKRKR"
BASE COUNT          241 a          588 c          612 g          250 t
ORIGIN      
        1 ggctccaaga ccaagtgagc cgggcctgac gcgacctcag ggtccgtcgc tgttcgagcg
       61 cgcccgcctc cggccggcgc gctcgcgcat ttaagcgatc gcacttaagc gatccggggc
      121 gaacgcggcc ggtacactgc gggcatgcgc gcggggcggg tcgtgcgagt caacgtggcg
      181 gccgagggcg gcgtgcccaa gcggccagtg gtcgcggcgc tgctgacggt gcacggcgtc
      241 gccggcgatc gccagcgcaa cctgaagtac cacggcgggc ccgaccgggc cgtgtgcctg
      301 tattcgcagg agcgcatcga cgcgctcatc gccgagggcc acccgatctc ggccggcagc
      361 accggcgaga acctgacgat cgccggcctc gcgtgggagg agctgcgcgt cggcgaccgc
      421 ctcgcggtcg gtgaggtcgc cgtcctcgag ctcaccgaac cgaccacccc gtgccgcacg
      481 atcgccggaa gttttaaaaa gggggagttc aagcgcatct ccgacgtgct ccaccccggc
      541 tggagccgct tctacgcgcg cgtggtgatc gaagggctgg tccgcgaggg cgcgcaagtg
      601 cgccgcctcg acccggacga cgcgccctga ccgcttggtg tagatccatg ggatgacgga
      661 caagcccctc gtcgcgcgct gcgagcccac ggtgctgacc ctcgagcccg gcatctactg
      721 gtggtgcgcc tgcgggcgct cgaagaccca gccgttctgc gacggcaccc acgacatcac
      781 cgacatcaag ccggtcaagc tgcgcatccg cgagacctcc gaggtcgccc tgtgcaactg
      841 caagcacagc ggcaacaagc cgcactgcga cgggacgcat gcgcgtatga ccgcctccga
      901 gccgggtacc tgagcacgcc ccggacgcca tccgggaagc gagcgaccct gtgccgagcg
      961 accgagcggc cggcactacc gcttgcgctt gcgcagcgag ctgaagaacc ccttcttctg
     1021 gctgacggcc tcaccaaagc tctcggccag ctcgcgcagc agctcttctt gttcctggct
     1081 gagcttttgc gggacggtga cctcgacatg gacgtggtgg tcgccgtggc cgcggccgcc
     1141 gagcacgggg atgcccttgc ctttgcggac gacgacgtgg ccgggctggg taccggcttc
     1201 gatctcgagg tcctggtcgc cttgcaggga cggtgtcttg acggtgcagc cgagcgcggc
     1261 ctgggccatc gagatcgaca ccttgctgtg gatgtcgaac tcgtcgcgct ggaagcgggg
     1321 gtcggcctcg acggcgacca cgacatagag gttgccggcg gggccgccct tggtgccggc
     1381 catgccgcgg ccggggatgc gcagggtctg gccgtcgtcg acgccggcgg ggatcgagag
     1441 cttgagggtg gtctcctttt ggaccacgcc ggtgccgccg cagccgcggc acgggctggc
     1501 gatcacctgg ccctcgccgt ggcagcgcgg gcacgcggtc tgcacggtga agaagccctg
     1561 gcggtggatc acctgaccct ggccgtggca ggtcgcgcag gcttgcggct tggtgccggc
     1621 ggcggccccg gtgccgtcgc actcctcgca ggcgtcgcgg cgcgggacgg tgacctcgcg
     1681 ctcgacgccg g
//
LOCUS       DASWNJ010001033         7261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_54690, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001033 DASWNJ010000000
VERSION     DASWNJ010001033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7261
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54690"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VGB85_17410"
     CDS             complement(<1..713)
                     /locus_tag="VGB85_17410"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HEY0135869.1"
                     /translation="MTREWLRVASGLALLAAASVVMTPGTAEACGGTFCDGQVPGPMP
                     VDQSGESVIFVRGDTLNEVHIQIQIDPNTNAEQFGWLVPLAEVPEFSVGSQPLFDQLR
                     AASVPQYDITTTFEDCGGGDGGAFTSGDPTSPNSSESEGASAGGTGEDDSGGPTVLKE
                     ETVGAFDVVVLQDTEVGPIKEWLLDNGYVWDANAEPILMQYLAEGNVIAAFKLNTDAN
                     VSDVHPITLRYPSNETCFPL"
     gene            complement(961..2763)
                     /locus_tag="VGB85_17415"
     CDS             complement(961..2763)
                     /locus_tag="VGB85_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HEY0135870.1"
                     /translation="MALIGAASLAMTPGTAEACGGTFCDGQIPGPMPVDQSGENVIFV
                     IGETELEVHIQISIDPNTNAEQFGWLVPLADVPEFSVGSQPLFDQIRAASVPQYDITT
                     TFESCDAFQTEGNFSISAGDDSGGGASTTGSQTSGVDEEPTVLKEETVGAFDVVVLQD
                     TEVAPIKQWLLDNGYVWDANAEPILMQYLAEGNVIAAFKLNTDANVSDVHPITLRYPS
                     NETCFPLRLTRIAAVEDMDIRVFVLSEDRAAPTNYRHVLVNPLKIDWLTRAANYKEVI
                     TQAVDAFEADGRAFVTEYAGPSNSVNTGPIYSANWDDTSFVGLDPIEVVNTLNTQGLG
                     DCYDSFSCVWNHPLIFPLLVEFLPAPADVDPAEFYSNLGSYAAQIDAVKWGNGDGFAA
                     ALLKRVIEPGMHADELLKTWPYLTRMYTTISPNEMMEDPIFHINPDLAEVPNLLTANN
                     YVLCNSDSVVTVPDGREFYIPGGQTWPAIPGEEWYAEEVQTVAIKGAPMTIVNNTSAI
                     TKKLTEWNLAHGWPYVPGETGTPTTSDTDGSGTDGTDGTGGTGGTGGSESTAGGLGDD
                     GGCGCRSDEQDGRQGALWLGLAALGLLGARRRRS"
     gene            complement(2942..4354)
                     /locus_tag="VGB85_17420"
     CDS             complement(2942..4354)
                     /locus_tag="VGB85_17420"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0135871.1"
                     /translation="MPSLPLDAIATLLDQGAADSAVRLLRSSWEPELPPDDLVRMYCM
                     WIRGLCETDELDSALTLAKRAASEFPREIDILIALGNVHDLFGELEEARGAFEVAIDV
                     DPAGALQHYNLGAVLERLGRETEAETCYRHANEADRGGGAMYEATSALGAMLRRQGRL
                     EEAEQVYDAYLAYDPIHVEILVEHGICLSDLERFEEAIERFNFALSIDPEHAGAQYNK
                     AITLYRVGKHEQAQAALEQARRLDPENALTLAVLGGWRLSASDCDLDEALGLLYGALD
                     LLERRYGGDAGNAGYCSLVVEEVFEALWQTGRKGEAREVARIAGQREWITPHILDSLN
                     EADHGRSPQVTIFTVLARAEAGERPAYWPENSDGYTTGLTVLACDEDEAREFSLEYLR
                     SIEPSPTVRFTIDVVPPRQPEPAVAAASASATGPAASTTGASGPGGGAGSGLDATGPA
                     QPRARGVFRVHAQRAYIYRS"
     gene            complement(4494..5327)
                     /locus_tag="VGB85_17425"
     CDS             complement(4494..5327)
                     /locus_tag="VGB85_17425"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0135872.1"
                     /translation="MSVQVAVITGASAGLGEAFARAVDVHPAFRGVQEIWLVARREER
                     LQALASALSHGRGVAVVADLASAQGVEQVLARARASDARVRLLVNNAGFGLLGPMLEQ
                     APEDLGRLLDLNVRAGTLLLRGLGPRMDAGAGVLQVASAAAYVPAPYFAAYAASKSYA
                     LALALALREEWRARGVSVCAVCPGPVRTEFFAVAGGRGGEVGEGAGVPASRVVALALR
                     DLLAGRAVSHAGIGPGVLARLAGVLPRGVLVRAAGRRNLRRGPLARTGPGGPSGGSGG
                     V"
     gene            complement(5324..6511)
                     /locus_tag="VGB85_17430"
     CDS             complement(5324..6511)
                     /locus_tag="VGB85_17430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEY0135873.1"
                     /translation="MSWTDPRGDIEALLPQLRQIRRDIHKHPELGFEETRTQALCRAW
                     LEERGYVPRVCAETGLIADLHPDAPGPVVALRADLDCLPMHEPTDIPHRSIHPNKAHK
                     CGHDGHTAILLGVADLLARYRDEVGGNVRLLFQPAEEGVRGGGAKVMVAEGALAGVAE
                     VYGLHNWPGFPKGQVHVKPGAMLAQVHTLKLTIYGKGGHASQPQICRDPIVAGAHLVS
                     ALQTVVARGLGYTGGAVLSITQFSSGTTHNVIPDTAQLQGTVRTFDPVVGERVLERLR
                     EVVAGTAASFGVKIDLEIEAGYPVTSNDPRCAAAVARVAAAQLGPAQVTEQDLPCAGG
                     EDFAYLSQAVPGAYFFVGARRPGEDTPVCHHPDFDFDDDLIPTGIGMFLGLVRDRLAE
                     LRG"
     gene            6565..7080
                     /locus_tag="VGB85_17435"
     CDS             6565..7080
                     /locus_tag="VGB85_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HEY0135874.1"
                     /translation="MPSGRGPLRSKALVLYEGTRTPHRSCGIALAETFDRPSAAYQSL
                     RRGGITGRGTCGAVVAGQLLLGEFLGDPDPTGKVTPALRAAMQSYDDKIAAGLGHGLG
                     PALVCNNLTAPHGDFMGPARHQFCTGLVGQVADIVDAVLREHGIEHHATPVPLADGSV
                     FDPEAAPSDMS"
BASE COUNT         1007 a         2785 c         2395 g         1074 t
ORIGIN      
        1 agcgggaagc aggtctcgtt cgacgggtag cgcagcgtga tcgggtggac gtcggagacg
       61 ttggcgtcgg tgttgagctt gaacgccgcg atcacgttgc cctccgccag gtactgcatc
      121 aggatcggct cggcgttggc gtcccacacg tagccgttgt cgagcagcca ctccttgatc
      181 gggccgacct cggtgtcctg caacaccacg acgtcgaacg ccccgaccgt ctcctccttg
      241 agcaccgtcg gcccgccgct gtcgtcctca ccggtgccgc cggcggacgc gccctcgctc
      301 tcgctgctgt tgggggaggt cgggtcgccg ctggtgaacg cgccgccatc gccgccgccg
      361 cagtcctcga aggtcgtcgt gatgtcgtac tgcggcaccg aggccgcgcg cagctggtcg
      421 aacagcggct gcgagccgac cgagaactcg ggcacctccg cgagcggcac cagccagccg
      481 aactgctccg cgttggtgtt cgggtcgatc tggatctgga tgtgcacctc gttgagggtg
      541 tcccccctca cgaagatcac gctctcgccg ctctggtcga ccggcatcgg gcccgggacc
      601 tgaccgtcac agaatgtgcc accgcaggcc tctgccgtgc ccggcgtcat caccacgctc
      661 gccgcggcca gcaacgccag accactcgca acccgcaacc actctcgagt cattgttcgc
      721 atcgtaagct ctcccgtaaa tccggtagga ccacacgatc ccacagcggg gccagggaat
      781 ccagccccgc tgtcacggcc agctcacggc gtgccgtcgg atggcgcgtt gacgcccgtg
      841 cccgcggaac gacgaacggc ccggcgatac gctcgccggg ccgtgtcgtc catatcgtct
      901 gtgtggtccg cgtcaccgcg cctggccttc gggacatgtt cccgacagcc gggatcgggc
      961 tcaactgcgg cgacgtcgcg cgccgagcag cccgagggcc gcgaggccga gccacagcgc
     1021 gccctgccgg ccgtcctgct cgtcgctgcg gcagccgcag cccccgtcgt cgccgagccc
     1081 gcccgcggtg ctctcgctgc cgccggtccc cccggtgccg ccggtgccgt ccgtgccgtc
     1141 cgtgccgctg ccatcggtgt cgctggtcgt cggggtgccg gtctcaccgg gcacgtacgg
     1201 ccagccgtgc gcgaggttcc actcggtcag cttcttggtg atcgccgacg tgttgttgac
     1261 gatcgtcatc ggcgcgccct tgatcgccac cgtctggacc tcctccgcgt accactcctc
     1321 gcccgggatc gccggccagg tctggccgcc ggggatgtag aactcgcggc cgtcgggcac
     1381 cgtcaccacg ctgtcgctgt tgcacagcac gtagttgttg gccgtcagca ggttcgggac
     1441 ctcggcgagg tccgggttga tgtggaagat cgggtcctcc atcatctcgt tcggcgagat
     1501 cgtcgtgtac atgcgcgtca ggtacggcca cgtcttgagc agctcgtccg cgtgcatccc
     1561 gggctcgatc acccgcttga gcagcgccgc cgcgaagccg tccccgttgc cccacttcac
     1621 cgcgtcgatc tgcgccgcgt agctcccgag gttcgagtag aactccgccg ggtcgacgtc
     1681 cgccggcgcc ggcaggaact cgaccagcag cgggaagatc agcgggtggt tccagacgca
     1741 gctaaagctg tcgtagcagt cgccgaggcc ctgggtattg agcgtgttga cgacctcgat
     1801 cgggtcgagg ccgacgaagc tcgtgtcgtc ccagttcgcg ctgtagatcg gccccgtgtt
     1861 gacgctgttg ctcgggcccg cgtactcggt cacgaacgcg cggccgtcgg cctcgaacgc
     1921 gtcgaccgcc tgcgtgatca cttccttgta gttcgccgcg cgcgtcagcc agtcgatctt
     1981 gagcgggttg accagcacgt gccggtagtt ggtcggcgcc gcgcggtcct cggacagcac
     2041 gaacacgcgg atgtccatgt cctcgaccgc cgcgatccgg gtcagccgca gcgggaagca
     2101 ggtctcgttc gacgggtagc gcagcgtgat cgggtggacg tcggagacgt tggcgtcggt
     2161 gttgagcttg aacgccgcga tcacgttgcc ctcggccagg tactgcatca ggatcggctc
     2221 ggcgttggcg tcccacacgt agccgttgtc gagcagccac tgcttgatcg gcgcgacctc
     2281 ggtgtcctgc aacacgacga cgtcgaacgc accgaccgtc tcctccttga gcaccgtcgg
     2341 ctcctcgtcg acgccgctgg tctggctccc cgtggtcgag gcgccaccac ccgagtcgtc
     2401 gccggcgctg atgctgaaat tgccctcggt ctggaaggcg tcacagctct cgaaggtcgt
     2461 cgtgatgtcg tactgcggga ccgaggccgc ccggatctgg tcgaacagcg gctgcgagcc
     2521 gaccgagaac tccggcacgt ccgcgagcgg caccagccag ccgaactgct ccgcgttggt
     2581 gttcgggtcg atcgagatct ggatgtgcac ctcgagctcg gtctcgccga tcacgaagat
     2641 cacgttctcg ccgctctgat cgaccggcat cgggcccggg atctggccgt cacagaacgt
     2701 gccaccgcag gcctccgccg tgcccggcgt catcgccagg ctcgccgcac cgatcagcgc
     2761 cacgccactc acaagccgca accaattcga agtcgatcgt cgcatcacta agttctccag
     2821 taatcgaggt agggcttcca cactcccacg gtccgccgcc ccgcgccagc ggccgagatc
     2881 cacaaaacac agttttcccg cacgccgggt gccgcgccgg gtgcagttcc ggagcgacgg
     2941 ctcaagagcg ataaatgtag gcccgctgcg cgtgcacccg gaacacgccg cgcgctctcg
     3001 gctgggccgg acctgtggca tcgagccccg agcctgcgcc cccaccgggc ccggaggcgc
     3061 cggtcgtgct cgctgccggc cccgtcgcgc tcgccgatgc ggctgccacc gccggctcgg
     3121 gctgccgcgg cggcacgacg tcgatcgtga agcgcaccgt cggcgagggc tcgatgctgc
     3181 gcaggtactc gagcgagaac tcgcgggcct cgtcctcgtc gcacgcgagc accgtcaggc
     3241 cggtcgtgta gccgtcggag ttctcgggcc agtacgccgg tcgctcaccg gcctcggcgc
     3301 gcgcgagcac ggtgaagatc gtgacctgcg gcgagcggcc gtggtcggcc tcgttgaggc
     3361 tgtcgaggat gtgcggcgtg atccactcgc gctggccggc gatgcgggcg acctcgcggg
     3421 cctcgccctt gcggccggtc tgccacagcg cctcgaacac ctcctcgacc accaggctgc
     3481 aataacccgc gttgccggcg tcgccgccgt agcgccgctc gagcaggtcg agcgcgccgt
     3541 acagcaggcc gagcgcctcg tcgaggtcgc agtcgctggc gctcaggcgc catccgccga
     3601 gcaccgcgag cgtcagcgcg ttctccgggt cgagccttcg cgcctgctcg agcgcggcct
     3661 gggcctgctc gtgcttgccg acgcgatata gcgtgatcgc cttgttgtac tgcgcccccg
     3721 cgtgctcggg gtcgatgctg agcgcgaagt tgaagcgctc gatcgcctcc tcgaagcgct
     3781 cgaggtccga gaggcagatc ccgtgctcga ccaagatctc gacgtggatc gggtcgtagg
     3841 cgaggtacgc gtcgtagacc tgctcggcct cctcgagccg gccctgccgc cgcaacatcg
     3901 cgccgagggc cgaggtcgcc tcgtacatcg ccccgccgcc gcggtcggcc tcgttggcgt
     3961 ggcggtagca ggtctcggcc tcggtctcgc ggccgagacg ctcgagcacc gcgccgaggt
     4021 tgtagtgttg cagcgcgccg gccgggtcga cgtcgatcgc gacctcgaag gccccgcgcg
     4081 cctcctcgag ctcgccgaac aggtcgtgca cgttgccgag cgcgatcagg atgtcgatct
     4141 cccgcgggaa ctcgctcgcc gcccgcttcg ccagcgtcag cgcgctgtcc agctcgtcgg
     4201 tctcacacag cccgcggatc cacatgcagt acatgcggac cagatcgtcg ggcggcagct
     4261 cgggctccca ggagctccgg agcaggcgca ccgccgaatc ggcggcgccc tgatcgagaa
     4321 gtgtggcgat cgcgtcgagc ggcagagagg gcatggcgac gtcgaggaga cctgttttta
     4381 cacgccgcgg ccgggctggc aatgactcgt atagccgtcc ctggggccag agaagccggg
     4441 acgcccgcca gttttgcggc cgggaccgcc gtccggccac gccctgcccg cgctcatacc
     4501 ccgccggagc cccccgaagg gcccccgggg cccgtcctgg cgagcgggcc ccggcggaga
     4561 tttcgccgcc cggccgcacg aacgagcacg ccccgcggca acactccagc cagacgcgcg
     4621 agcacacccg ggccgatccc cgcgtgcgag acagcccggc ccgcgagcaa gtcgcgcagc
     4681 gctagggcca ccacccgcga cgccggcacc ccggcgccct cgcccacctc cccgccgcgc
     4741 cccccggcga ccgcgaagaa ctccgtccgc accggccccg gacacaccgc gcacaccgac
     4801 accccgcgcg cccgccactc ctcgcgcagc gccagcgcca gcgccagcgc gtagctcttg
     4861 ctcgccgcgt aggcggcgaa gtatggcgcc gggacgtagg ccgccgccga ggcgacctgc
     4921 agcaccccgg ccccggcgtc catccgcggc ccgagcccgc gcagcagcag cgtgcctgcg
     4981 cgaacgttca gatcgagcag acggccgaga tcctccggcg cttgctcgag catcggcccg
     5041 agcagcccga agccggcgtt gttgaccagc agccgtacgc gcgcgtcgct cgcccgggcc
     5101 cgcgcgagca cctgctcgac cccctgcgcg ctcgccaggt cggcgaccac cgcgacccca
     5161 cgaccgtggc tcagcgccga cgcgagcgcc tgcaggcgct cctcgcggcg cgcgaccagc
     5221 cagatctcct gcaccccgcg aaacgccggg tgcacgtcga ccgcccgcgc gaacgcctcg
     5281 ccgagccccg ccgacgcgcc ggtgatcacc gcgacctgca cgctcatccg cgcagctcgg
     5341 ccaggcggtc gcgcacgagc ccgaggaaca tgccgatgcc ggtcgggatc aggtcgtcgt
     5401 cgaagtcgaa gtcggggtga tgacacaccg gcgtgtcctc gcccgggcgc cgcgccccga
     5461 cgaagaagta cgcccccggc accgcctgcg acagatacgc gaagtcctcc ccgcccgcgc
     5521 agggcaggtc ctgctcggtc acctgcgccg gcccgagctg cgccgcggcc acgcgcgcca
     5581 ccgcggccgc gcagcgcggg tcgttgctgg tcaccggata gcccgcctcg atctcgaggt
     5641 cgatcttcac cccgaagctc gccgccgtgc ccgcgaccac ctcgcgcagg cgctcgagca
     5701 cccgctcgcc gaccacgggg tcgaaggtcc gcaccgtccc ttgcagctgc gccgtatcgg
     5761 ggatcacgtt gtgcgtcgtg cccgacgaga actgcgtgat cgacagcacc gcgccgcccg
     5821 tgtagccgag cccgcgcgcg accacagtct gcaacgcgct gacgaggtgc gcccccgcga
     5881 cgatcgggtc gcggcatatc tgcggctgac tcgcgtgccc acccttgccg taaatcgtca
     5941 gcttgagcgt gtgcacctgc gccagcatcg cccccggctt gacgtgcacc tgtcccttgg
     6001 gaaatcctgg ccagttgtgc aggccgtaga cctcggccac cccggcgagc gccccctcgg
     6061 cgaccatcac cttcgccccg ccgccgcgca cgccctcctc cgccggctga aacagcaggc
     6121 gcacgttgcc gccgacctcg tcgcggtagc gggccagcag atcggcgacg ccgagcagga
     6181 tcgccgtgtg cccgtcatga ccgcacttgt gcgccttgtt cgggtggatc gatcgatgcg
     6241 ggatgtcggt cggctcgtgc atcggcaagc agtcgaggtc cgcccgcagc gccacgaccg
     6301 gccccggcgc gtccgggtgc aggtcggcga tcaggccggt ctcggcgcac acccggggta
     6361 cgtacccccg ctcctcgagc cacgcgcgac acagcgcctg cgtacgcgtc tcctcgaagc
     6421 cgagctccgg gtgcttgtgg atgtcgcgcc ggatctgccg cagctgcggc agcagggcct
     6481 cgatgtcgcc tcgcgggtcg gtccaggaca tgccccacgg tagacgatcc cccgccggcg
     6541 cgcgagcgtg gcacactgtc ccgcatgccc tccggtcgcg gtcccctgcg cagcaaggcc
     6601 ctcgtgctct acgagggcac ccgcaccccg caccgctcgt gtggcatcgc cctcgccgag
     6661 accttcgacc gtccgagcgc cgcctaccag agcctgcgcc gcggcggcat caccggccgc
     6721 ggcacctgcg gtgcggtcgt cgccggccag ctgctgctcg gtgaattcct cggcgacccc
     6781 gacccgaccg gcaaggtcac ccccgccctg cgcgccgcca tgcagtccta cgacgacaag
     6841 atcgccgccg gcctcgggca tggcctcggc cccgcgctcg tgtgcaacaa cctcaccgcc
     6901 ccgcacggcg acttcatggg ccccgcgcgc caccagttct gcaccggact cgtcggccag
     6961 gtcgccgaca tcgtcgacgc cgtgctgcgc gagcatggca tcgaacacca cgcgaccccc
     7021 gtccccctcg ccgacggcag cgtgttcgac cccgaggctg ccccttcgga catgtcctga
     7081 gcgcgcgccg cggtccgccg ggccgcgcgc gggcgcatcc acagccgagc tgcggccagg
     7141 tgacggccga gttgccagga cgcaggcggg cgtgccaccc ttgccgccat gcagacaccg
     7201 cccgcagacg tgcccgccgc tccgatggcc gccccgggac cgaccgtgcc gccgccgcag
     7261 g
//
LOCUS       DASWNJ010001034         3988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_370884, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001034 DASWNJ010000000
VERSION     DASWNJ010001034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370884"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VGB85_17440"
     CDS             <1..410
                     /locus_tag="VGB85_17440"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY0135875.1"
                     /translation="NGQRVTLGELVRTRCVVLYFYPKDDTPGCTTEACSFRDHYEVLR
                     TAGAEVVGVSSDDVHAHEAFAAKYRLPFTLLSDPDGSVRASYGVKRTLGLVDGRVTFV
                     IDRQGIVRHVFSSQIRPKKHVEEALRIVQQLSR"
     gene            635..1012
                     /locus_tag="VGB85_17445"
     CDS             635..1012
                     /locus_tag="VGB85_17445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135876.1"
                     /translation="MSLLANMEHLAAASGVVAVTIDSPQHPRAFRGVETWSPVLDAAL
                     GLFEKTDEDTIRLVIGQHTIIVQKEHGQTVAVVLPTGHAIAKSLRRMIRRLSKKERGP
                     LQPRPQADASAGQAPTAPHSEAA"
     gene            1224..2450
                     /locus_tag="VGB85_17450"
     CDS             1224..2450
                     /locus_tag="VGB85_17450"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0135877.1"
                     /translation="MRRLFRPRRSAPTLCTLLVCALAGCDAGDAAPPLEVEVSGPAPR
                     RARDDDPTRRPPDTNPRDAMTFHFIDIGQGDATLLEFPCGAMLIDTGGEQNELFDSNK
                     ALLAYLDAFFARRKDLDRTLDLLLITHPHIDHNRSAVAVLERYKVRGVIDNGADTPHS
                     HNDGTADPGATQQMAVHAWLRDHPRDPAISYLGVGASNVPVEGLTNAVVDPVAGCAAS
                     AVDPKVTLLWGTVTEDMRTYGDNPNNHSVVARVDFGRASALFTGDLELIGLSRLAARY
                     EDHPELLDVDIYQVGHHGSKNATIHYQMQMMSPRVAVISMGPYERNIPWTARKYGHPN
                     IIALEHLAHPHHGVRAHRSPIEAWVGIKGAWKDEKKEVFERRTVDRAIYATGWDGTVT
                     ITANANGWLDITTLGR"
     gene            2463..2657
                     /locus_tag="VGB85_17455"
     CDS             2463..2657
                     /locus_tag="VGB85_17455"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEY0135878.1"
                     /translation="MATDERHDHEHDHGHAHEHVHGPGCAHDHEHAGPRPYVREQPKV
                     GRNDPCVCGSGKKFKKCCGA"
     gene            complement(2664..>3988)
                     /locus_tag="VGB85_17460"
     CDS             complement(2664..>3988)
                     /locus_tag="VGB85_17460"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY0135879.1"
                     /translation="ALGPVQLVELPEGAPPRVRLEHGGRSLVLDAEALAAGNVPLARR
                     IAAELLDPAETAEALERFAREAPRQDALRRLTRAMLETSSVERLRQITLLGLTSSAAL
                     GLDRAALFVYDEEARVLVGSSAIGPSDADEARRIAEAAELRDGSLEPLFAELSTNAEE
                     SRFEEFVRSLECDLVRAVEGDEIVAALAASAPLVFTRANPLSPVIAALAPPEEFLVSA
                     IKLRGRPLGVVVADNRYSGAPLEREALGFIGFLLDAAALVLENLRLLESVETLARHDA
                     LTGLFNRREFETRMQEEQSRAQRLGSQCALLLLDLDHFKALNHDQGHKAGDELLQAVG
                     VLLRSTLRSHDIVARFDGDEFAVLVTDTNAEQAMMIARRIGAQALEQGISLSIGGSLW
                     PRDNEDTSVLFAEADACLYAAKRDGRGRVHVEGVAGAVFVPSDAVSVG"
BASE COUNT          670 a         1490 c         1261 g          567 t
ORIGIN      
        1 agaacggcca gcgcgtcacc ctcggtgagc tcgtccgcac ccgctgcgtg gtgctctact
       61 tctatcccaa ggacgacaca cccggctgca ccaccgaggc gtgcagcttt cgcgaccact
      121 acgaggtgct gaggaccgcc ggcgccgagg tggtcggcgt cagctccgac gatgtccacg
      181 cgcacgaggc cttcgccgcc aagtatcgcc tgccgttcac gctgctgagc gaccccgacg
      241 gcagcgtgcg cgcgagctac ggagtgaagc gcacgctcgg cctcgtcgac ggccgcgtca
      301 ccttcgtcat cgatcgccag ggtatcgtcc gacacgtctt ctcctcccag atccgcccga
      361 agaagcacgt cgaggaggcg ctgaggatcg tgcaacagct gtcgcgctga cccggacgcg
      421 tgtcctcttc gctctctgag cgcgcatgca ccgcctgcag cacgcgtcac aacaagttgg
      481 tcgctcacac aaacatgggt gggagacggt ggcacgcctc gcgaaccggg cccatggttg
      541 aactgcgaac gagcctccgg atgcgggcca tggggtccgt cgacaggccg ggcgccgact
      601 tacgtcgggc cgcgggttcg cgcagggagc caccatgagt ctacttgcca acatggaaca
      661 tctggcggcg gcaagcggtg tcgtcgcggt caccatcgat tcgccgcagc acccccgggc
      721 gttccgcggg gtcgagacct ggtccccggt gctggacgcc gcgctgggtc tcttcgaaaa
      781 gaccgacgag gacacgatcc gtctcgtcat cggtcaacac acgatcatcg tgcagaagga
      841 gcacggccag accgtcgcgg tggtcctacc caccggccac gcgatcgcca agagccttcg
      901 tcgcatgatc cgccggctct ccaagaagga gcgcgggcca ctgcagccac gcccgcaggc
      961 cgatgccagc gcgggccagg caccgactgc gccacactcc gaagctgcct gaccatgtga
     1021 tccacgtgat ccacgtgata atggcccttc gcgcggcgta gcccgctact acaacttcat
     1081 acaatcccac caaatcggga tcagcggcgg ctgcgcagcg atgcgagccg ccgttgtcgc
     1141 gttcgcagaa cgaggtcggc ccggccggca gcgaacgtga cgacgtgatc tgactctgcc
     1201 gcgtcgctgg tataatcccg cacgtgcgcc gtctctttcg ccctcgtcgc tccgccccga
     1261 ccctgtgcac gctcctcgtg tgcgcgctcg cggggtgtga cgccggagac gctgcgccgc
     1321 cgctcgaggt cgaggtctcg ggcccggccc cgcgtcgcgc tcgcgacgac gatcccacca
     1381 ggcgtccgcc ggacaccaac ccgcgcgacg cgatgacctt tcattttatc gacatcggtc
     1441 agggcgacgc gacgctgctc gagttcccgt gcggcgcgat gctcatcgac accggcggcg
     1501 aacaaaacga attgttcgac agcaacaagg cgctgctcgc ttacctcgac gccttcttcg
     1561 cgcgccgaaa agatctcgat cgcacgctcg acctgctgct gatcacccac ccgcacatcg
     1621 accacaaccg gtcggccgtc gcggtgctcg agcggtacaa ggtccgcggc gtgatcgaca
     1681 acggagcgga cacgccccac agccacaacg acggcacggc agatcccggc gccacgcaac
     1741 agatggctgt acatgcgtgg ctgcgcgatc atccgcgaga tcctgcgatc tcctatctcg
     1801 gcgtcggggc cagtaatgtc cccgtcgagg gtcttacaaa tgcggtcgtc gatccggtcg
     1861 ccggctgcgc agcctcggcg gtcgatccga aggtcaccct gctgtggggc acggtcaccg
     1921 aggacatgcg cacctacggt gacaatccca acaaccacag cgtcgtcgcc cgcgtcgatt
     1981 ttggcagggc gtccgcgctg ttcacgggag atctcgagct gatcgggctg tcacgcctgg
     2041 ccgcccgcta cgaggaccac cccgagctgc tcgacgtcga tatctatcaa gtcggacatc
     2101 acggctccaa gaacgcgacg atccactacc agatgcagat gatgagcccg cgggtcgccg
     2161 tgatctcgat gggcccctac gagcgcaaca tcccgtggac ggcccgcaag tacggccacc
     2221 ccaacatcat cgccctcgag cacctcgccc acccgcacca cggcgtccgc gcgcaccgca
     2281 gcccgatcga ggcctgggtc ggcatcaagg gcgcctggaa ggacgagaag aaggaggtct
     2341 tcgagcgccg caccgtcgac cgtgcgatct acgcgaccgg ctgggatggc accgtcacga
     2401 tcaccgccaa cgccaacggc tggctcgaca tcaccaccct gggccgctag cctgacagcc
     2461 ccatggccac cgacgagcga cacgaccacg agcatgacca cggccacgcg catgaacacg
     2521 tgcacggccc cggctgcgcc cacgaccatg aacacgccgg cccgcgcccc tacgtgcgcg
     2581 agcagcccaa ggtcggtcgc aacgacccct gcgtgtgcgg cagcggcaag aaatttaaaa
     2641 aatgctgcgg cgcgtgaggg gcttcagccg acgctgaccg cgtcgctcgg cacgaacacc
     2701 gcgcccgcca cgccctcgac gtggacgcgg ccgcgaccgt ctcgcttggc cgcataaagg
     2761 cacgcgtcgg cctcggcgaa cagcaccgag gtgtcctcgt tgtcgcgcgg ccacagcgag
     2821 ccgccgatgc tgagcgagat cccctgctcg agcgcctggg cgccgatccg ccgggcgatc
     2881 atcatcgcct gctccgcgtt ggtgtccgtc accagcaccg cgaactcgtc gccgtcgaag
     2941 cgcgccacga tgtcgtgcga ccgcagagtc gagcgcagta gcaccccgac cgcctgcagc
     3001 agctcgtccc ccgccttgtg gccctggtcg tggttgagcg ccttgaagtg gtcgaggtcg
     3061 agcagcagca gcgcgcactg cgagccgagc cgctgcgccc gcgactgctc ctcctgcatg
     3121 cgcgtctcga actcgcggcg gttgaacagc cccgtcaagg cgtcgtgccg cgcgagcgtc
     3181 tcgacgctct cgagcaaccg caggttttcg agcaccagcg ccgccgcgtc gagcaaaaag
     3241 ccgataaagc ccagtgcctc gcgctcgagc ggcgcgcccg agtaacggtt gtcggcgacg
     3301 acgaccccga gcggccgccc gcgcagcttg atcgcgctga ccaggaactc ctccggcggg
     3361 gccagggcgg cgatcacggg gctcagcggg ttggcccgcg tgaacaccag cggcgcgctg
     3421 gcggccagcg ccgcgacgat ctcgtcgccc tcgaccgcgc gcaccaggtc gcactcgagg
     3481 ctgcgcacga actcctcgaa gcgcgactcc tcggcgttcg tgctcagctc ggcgaacagc
     3541 ggctcgaggc tgccgtcgcg cagctccgcc gcctcggcga tccttcgcgc ctcgtcggcg
     3601 tcggacggcc cgatcgccga gctcccgacg agcacccgcg cctcctcgtc gtagacgaac
     3661 agcgccgccc gatcgagccc gagcgccgcg ctcgaggtca gcccgagcag cgtgatctgg
     3721 cgcagacgct cgacgctcga cgtctcgagc atcgcccgcg tcagccggcg cagcgcgtcc
     3781 tgccgcggcg cctcgcgggc gaagcgctcg agcgcctccg cggtctcggc cggatcgagc
     3841 agctccgccg cgatcctgcg cgccagcggc acgttgccgg ccgccagcgc ctcggcgtcg
     3901 agcaccagcg agcgaccgcc gtgctcgagc cggacccgcg gcggcgcgcc ctcgggcaac
     3961 tcgaccagct gcaccggccc gagcgcgg
//
LOCUS       DASWNJ010001035         5561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_196207, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001035 DASWNJ010000000
VERSION     DASWNJ010001035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196207"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2266
                     /locus_tag="VGB85_17465"
     CDS             <1..2266
                     /locus_tag="VGB85_17465"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0135880.1"
                     /translation="WEYLRLGEYFIAEQRLSIIRHVESSAEPRPLVIAKPRTVLFAYS
                     NPKSRYYDGDTHQKRIVTAIQELDVIVDPALTCTHDELERLLCNREGQVFHYLGHGEE
                     GGSRAAASLTLHGDGGKEVQACSAETLAKWVRDRPRDLVVLGSCFGGAVPKGGLLLSV
                     GGRIVHASATPVVAMQMEVPQEFSTAFAARFYRELKRVDHDIEVAVFMARRAECHERH
                     MFGIPVLLSDARNLPALGALQRPIGEPERWASFAVEASLPADEARSLWRASAAVSPAA
                     SRAVDRALEINARAGHATGYPPPPADSSALEEGLAELERSLAEPAAREVLRGLEPAVP
                     AAAMEPVALARGRVVPVNDVPLSARLTSELCRAAIDRIDRNLSLPEGLAARIVGELRA
                     GNHVMLTGPVGTGKTSLARQVTEALGYRVHLETASADWTRFEVLGGFWPTPDANADRL
                     TFKFRAGVFLEAVQANWRPQPTVNGETPWRREPCGEEHGVWLVLDELNRADMDRALGG
                     VFTALESFKLRVPATDADGRTVEIPIPKDFRVIATINIADRHFLFRLSDALKRRFAFV
                     HVPVTTAWNEEWQLLCRRVCLPAGMDVSDLRRFVALVRLLHPLGTALVQAALKFYAAS
                     NFLERKPPEWPLAQAVEGAILPNLEDLRDAGLQVLCTWAETTDPGRLMGAVKDALPPD
                     LSMIEAELHALAELPSDVSPAWLDGDDAIPDRSDGDRLAAWIARRVARPPGVVTLSRL
                     KAAIAGLRQGATGH"
     gene            2355..3437
                     /locus_tag="VGB85_17470"
     CDS             2355..3437
                     /locus_tag="VGB85_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135881.1"
                     /translation="MFLQAGSGRVLDLALQGLFQRPSGDLPKPERMLANLLALLQRLI
                     DRELPALTRSMRVETTIVGTWTRTPQGGRVDGLASLRASAGAMPELWRVQRVDRNIDT
                     PVNLFVAAVLRTTSARIEAVSALYRRHGLNVPDILNDTGVALRQFLREHPLGSVAIPA
                     DAVPGSYWRAAARRVAEIRRIGSLVQWWDELQKVDLIALHELFAVEGETLAELSIHSV
                     YEMMVALGLVCALSLRLRSIASPDPEALRFAGHAVVVELRFGVRGPATKMRGFTASMK
                     ISSDSAPPATTLIDARNCSAASLPLYALYLEHACRQEACNGLLVAPEPAGAPEPGAVR
                     WVAMPADSDVPSLIRRWLNVLALDPR"
     gene            3460..4827
                     /locus_tag="VGB85_17475"
     CDS             3460..4827
                     /locus_tag="VGB85_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135882.1"
                     /translation="MMPLPSRIFVISDLHLGPVGPLRIFHAGAALARFIRSCAAVLGR
                     VELVILGDALDYLQFTTELAFTAAVGEQKTTEILNANPEVFDALASYMSTPDKSLVWF
                     IGNHDLELIFPSVRAQIVARIGATPTWHLTHTAREYELPGGAKFHLVHGNDPDAFNRV
                     DYTNLAAVAAGGGDPTRVYPIGSRLVDEVLNRLKLKGHGYIDLLKPEVGVALPLALAL
                     WPSEVGKWAGIKYIGKGTRQTVIDAIFTAISGPRGSFGRGSGDGRLTDVDPVLTSILA
                     TIYAGDPTIGGDAYLDWLDRPRAVAAPAAGGQGTFGAPARVSRALLLGAVRKHSAYDA
                     FDVYGADEFSDKSEYVAADGVVLEVVGHTHLARVRERGGSYYINTGTWADLMRLPTLA
                     RVDFDAMVTTLLDHFERPETGPAWLRPFRQLTFVDIALPGGTKWTARLRRWPEDDAIT
                     LAACP"
     gene            complement(4931..5122)
                     /locus_tag="VGB85_17480"
     CDS             complement(4931..5122)
                     /locus_tag="VGB85_17480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135883.1"
                     /translation="MLAACGGGTASGGEFSRTLTCDKVAEYAQRWPGQDIKLGPACKA
                     ANETCPGLVPDPETVSGSR"
     gene            complement(5201..>5561)
                     /locus_tag="VGB85_17485"
     CDS             complement(5201..>5561)
                     /locus_tag="VGB85_17485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135884.1"
                     /translation="GQILVIPKSSVSSVRAHVAPPVPTQNGGGLIAGGSVLTALGVPV
                     FLTGIVFVAICPSCTYLHIPMLVVGAAGLGAGIPMITRGVQRRTAFQKAMEENRVIPM
                     VSRTPYHGWTGGLQIRF"
BASE COUNT          844 a         1845 c         1937 g          935 t
ORIGIN      
        1 ttgggagtac ctgcggcttg gcgaatattt catcgcggag cagcggctct cgatcattcg
       61 ccatgtggag agctccgccg agccgcggcc gctcgtcatc gccaagccgc ggaccgttct
      121 gttcgcgtac tcgaatccga agtcgcggta ttatgacggc gatacgcacc aaaagcggat
      181 cgtcacggcg attcaggagc tcgacgtcat cgtcgatcct gcgctcacct gcacgcacga
      241 cgagctcgag cgcctgctgt gcaatcgcga gggacaggtg tttcactacc tcggccacgg
      301 tgaggagggg ggctcgcggg ccgcggccag cctgactctg cacggggatg gcggcaagga
      361 ggtacaggcc tgttcggcag agacgctggc gaagtgggta cgagaccgcc cgcgcgacct
      421 cgtcgtcctc ggctcctgct ttggtggggc ggtgccgaag ggcgggctgc tgctgagcgt
      481 cggcggacgg atcgttcatg cgagcgcgac gcccgtcgtg gcgatgcaga tggaggtgcc
      541 gcaggaattc agcacagcgt tcgctgcgcg cttctatcgc gaattgaagc gcgtcgacca
      601 cgacatcgag gtcgccgtgt tcatggcgcg gcgggccgaa tgccacgagc gccatatgtt
      661 cgggatcccg gtgctgctca gcgatgcccg caacctccct gcgctgggcg ccctgcagcg
      721 gccgatcggc gagcccgagc ggtgggcgag cttcgcggtc gaggcttcac taccggcgga
      781 cgaggcgagg tcgctgtggc gtgcgagcgc cgccgtgtcg cccgcggcgt ccagagcggt
      841 cgaccgcgcc ctcgagatca acgcgcgcgc cggccacgcg acaggctatc cgccgccgcc
      901 cgccgattcg tcggcgctcg aggaaggact cgcggagctc gagcgctcac tcgccgagcc
      961 ggccgcccgc gaggtcctcc gcggcctcga gccggccgtc ccggccgcgg cgatggagcc
     1021 ggtggcgctg gcgcgcggac gcgtcgtgcc ggtcaacgac gtcccgctgt cggcgcgcct
     1081 cacgagcgag ctgtgccgcg ccgcgatcga ccggatcgac cgcaacctca gcctgcctga
     1141 gggcctcgca gcgcggattg tcggcgaact gcgggcggga aaccatgtga tgctgacagg
     1201 gccggtcggc accggcaaga cctcgctcgc ccgccaggtc accgaggcgc tcggctaccg
     1261 tgtgcatctc gagaccgcct cggccgactg gacccgcttc gaggtgctcg gcggtttttg
     1321 gccgacgccc gacgccaacg cggaccggtt gacgttcaag ttccgtgcgg gcgtgtttct
     1381 tgaggcagtg caggccaatt ggcgcccgca gccgaccgtc aacggggaga ctccatggag
     1441 gcgcgagccc tgcggcgaag agcacggggt ctggctcgtg ctcgacgagc tcaaccgcgc
     1501 cgacatggac cgggcgctcg gtggagtgtt caccgcgctg gagtcgttca agctgcgcgt
     1561 cccggcgacc gacgccgacg ggcgcaccgt cgagatcccg atcccgaagg actttcgcgt
     1621 catcgccacg atcaacatcg ctgaccgcca cttcctcttt cgcctgtccg acgcgctcaa
     1681 gcgacgattt gcgtttgtcc acgtgccggt gacaactgcg tggaacgagg agtggcaatt
     1741 gctgtgccgc agggtctgcc tgcccgccgg catggacgtc agcgacctgc ggcgattcgt
     1801 cgcgctggtg cgcctgctgc atccgctcgg gacagcgctc gtccaggccg cgctgaagtt
     1861 ttatgccgct agcaactttc tcgagcgcaa gccaccggag tggccgttgg cgcaggccgt
     1921 cgagggcgct atcttgccca acctcgagga cctgcgtgat gctggcctgc aggtgctgtg
     1981 tacctgggcc gagacgacgg atccgggtcg tctgatgggg gcggtaaagg acgcgctgcc
     2041 gcccgacctg tcgatgatcg aggccgagct acatgcgctg gccgaattgc cgtccgacgt
     2101 atcgccggcc tggctcgacg gcgacgacgc catcccggat cgcagcgacg gcgaccggct
     2161 cgcggcgtgg atcgcccgtc gagtcgcacg tccccccggt gtcgtcacgc tgtcccgatt
     2221 gaaggccgcg atcgcgggat tgaggcaggg tgcgacgggc cattgatcgg tttggctggg
     2281 gcgcaggtcc acaagaccca accccgccga tcgccggtcc gcaggcggag cgtgcgcgtg
     2341 cgctcgcatt ttggttgttc ctgcaggccg gctcggggcg tgtcctggac ctcgcgctgc
     2401 aggggctatt tcagcgtccg agcggtgatc tccccaagcc cgagcgcatg ctcgccaacc
     2461 tgttggccct gctgcagcga ctgatcgatc gtgagttgcc ggcgctgact cgcagcatgc
     2521 gagtcgagac gacgatcgtg gggacgtgga cgcggacgcc gcagggcgga cgcgtcgacg
     2581 gcttggccag cctgcgggcg agcgccggcg ctatgccaga actgtggcgg gtacagcggg
     2641 tggatcgaaa catcgacacg ccagtcaacc tgtttgtcgc ggcggtcctg cgcaccactt
     2701 cggcgcgtat cgaagcggtc agcgcgctgt accgccggca cggcctgaac gtcccggaca
     2761 tcctcaacga caccggcgtc gccctgcgcc agttcctgcg cgagcacccg ctggggagcg
     2821 tcgccatccc cgccgatgcg gtccccggga gctactggcg ggcagccgct cggcgggtcg
     2881 ccgagatacg aaggatcggt tcactcgtgc agtggtggga cgagttacag aaggtcgacc
     2941 tgatcgcgct tcacgagctg ttcgctgtgg agggcgagac gctcgccgag ctgtcgatcc
     3001 atagcgtcta cgagatgatg gtcgcgcttg gcctcgtctg tgcgctttcc cttcgtttgc
     3061 gctcgatagc ctcccctgac cccgaagcgc tgcgattcgc tggtcacgcg gtggtggtcg
     3121 agctgcggtt cggcgtgcgc ggtccggcca caaagatgcg tggttttaca gcgagcatga
     3181 agatctccag cgacagcgcg ccaccggcga cgacgctgat cgacgcccgc aactgcagtg
     3241 ccgcctcgct cccgctctac gccctgtacc tcgagcatgc gtgccgccag gaggcctgca
     3301 acgggctcct ggtcgcgccg gagcccgccg gcgcgccgga gccgggggca gtacggtggg
     3361 tcgctatgcc cgcggactcc gacgtgccgt cactgatacg acggtggctc aacgtcctcg
     3421 cgcttgaccc tcggtgactc gtcgccgcac catgaggcca tgatgccgtt accctcgcga
     3481 atcttcgtca tcagcgatct tcatctcggc cctgtcggcc cgctgcggat cttccacgcg
     3541 ggggcggcgc tggcccggtt catccggagt tgcgcggctg tgctgggccg ggtcgagctg
     3601 gtcatcctcg gcgacgctct cgactaccta caatttacga cggagctcgc gttcaccgca
     3661 gccgtcgggg agcagaagac gacggagatc ttgaacgcca accccgaggt gttcgatgcg
     3721 ctcgcgtcct acatgtcgac cccggataaa tcgctggtgt ggttcatcgg caaccacgat
     3781 ctcgagctga tcttccccag cgtgcgcgcg cagatcgtgg cgcggatagg ggcgacgccg
     3841 acatggcacc tcacgcacac ggcgcgggag tacgagttac cgggcggcgc caaattccat
     3901 ctcgtgcatg gcaacgatcc tgacgccttc aaccgcgtcg actatacgaa cctcgcggcg
     3961 gtggcggccg gtggtggtga tccgacgcgg gtttatccca tcggctcgcg tctggtggac
     4021 gaggtgctca accggctcaa gctgaagggt cacggttaca tcgacctgct caagcccgag
     4081 gtcggggtcg cgttgccgct ggcgctggcg ctgtggccca gcgaggtcgg caaatgggcc
     4141 ggcatcaagt acatcggcaa agggacgcgg cagacggtga tcgatgccat ctttaccgca
     4201 atcagcggac cgcgcggcag ttttggccgc ggctcgggcg acgggcggct gaccgacgtg
     4261 gaccctgtgc tcacgagcat tctagcgacg atatacgccg gcgatcccac gatcgggggc
     4321 gacgcgtacc ttgattggct cgatagaccg cgcgcggtag ccgcgcccgc ggctggtggg
     4381 cagggcacct tcggcgctcc ggccagggtg tcgcgggccc tgcttctcgg ggctgtccgc
     4441 aagcacagcg cctacgatgc gttcgacgtg tacggcgccg atgaattctc ggacaaatcc
     4501 gagtacgtgg ccgcggatgg ggtcgtcctc gaggtcgtcg gccacacgca cctcgcccgc
     4561 gttcgcgaac gaggcggtag ttactatatc aatacaggca catgggccga tctgatgcgg
     4621 ctgcccacgc ttgcgcgcgt ggacttcgac gcgatggtga cgacgctgct cgatcacttc
     4681 gagcgaccag agaccggacc ggcgtggctg cgtccgttcc ggcaacttac gtttgtcgac
     4741 atcgcgctgc ctggcgggac caagtggacc gcacggctgc gtcggtggcc cgaggatgac
     4801 gcaatcacgc tcgcggcgtg cccgtgacgc gtcccgacca ggttgatgca ggccctcgct
     4861 tgtgctctgc caaatgtggc gacccctcat cacacagcac gctcgaccga cgcagtcgcg
     4921 ttgccggcga tcaccgggag ccggataccg tctccgggtc ggggaccagg ccggggcagg
     4981 tctcgttcgc ggccttgcag gcgggtccga gcttgatgtc ctggccgggc cagcgctgcg
     5041 cgtactcggc gaccttgtcg caggtcagcg tgcgcgagaa ctcgccgccg ctcgccgtcc
     5101 ctcctccgca ggcggccaac acgagcagga gcggtcgggg atgtgtgacg accgcgaacg
     5161 tagactgggc gtcggggccc ggcaaggcga cgccgggaga tcagaaccgg atctgcagtc
     5221 cgccggtcca cccgtgatac ggggtccgcg agaccatcgg gatgacgcgg ttctcttcca
     5281 tggctttctg gaaggcggtt cgtcgctgga ctcctctggt gatcatcggg atcccggccc
     5341 cgaggccggc ggcgccgacc accagcatcg ggatatggag gtacgtgcac gacgggcaga
     5401 tcgcgacgaa tacgatcccg gtgaggaaga ccggcacacc gagcgcggtc aggacgctac
     5461 cgccggcgat caggccgcct ccgttctggg tcgggacggg cggcgcgacg tgggcgcgga
     5521 ccgaggagac gctgctcttc gggatcacga ggatctgccc g
//
LOCUS       DASWNJ010001036         4751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_401351, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001036 DASWNJ010000000
VERSION     DASWNJ010001036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401351"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VGB85_17490"
     CDS             <1..744
                     /locus_tag="VGB85_17490"
                     /inference="COORDINATES: protein motif:HMM:NF021607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HEY0135885.1"
                     /translation="TDETGKTALPYWLEWDTGPVNAFVWVRVPTIAADDITTIRMYYG
                     NPNAAAGSDGFATFLFFDDFEAASLDAAKWKTTAPVSINFGRLSVTAGSVYSVKPVAS
                     FPDTWAEARMVWKPEIGNGYPSGLITSQGQAPGPGIQYFNRQANAAIVFDGKKFLAEK
                     MVQSANGENPVIMGIAADDTDTYFSYNRGFPESPSDQLAFTYYLLFGHPYGKSGDMTN
                     VGDLDVDWVLVRRHADMDPTTAVGGEKTP"
     gene            complement(770..1204)
                     /locus_tag="VGB85_17495"
     CDS             complement(770..1204)
                     /locus_tag="VGB85_17495"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY0135886.1"
                     /translation="MKEIRTELVIAASAGRVWDILVDFARYPEWNPLTPEATGELHPG
                     STLRLRVVAGREMRFAPVVQRVEAPYMLRWRGVIGHRLVFSGEHWFSIEPLGPQQVKF
                     MQGEVYSGLLVPLLARTLERDARPAFEAMNRTLQRRAEQTSG"
     gene            1295..1513
                     /locus_tag="VGB85_17500"
     CDS             1295..1513
                     /locus_tag="VGB85_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04563 family protein"
                     /protein_id="HEY0135887.1"
                     /translation="MTTPPGPQDPATSDKRKQSLYFPEQMLKEIQEEAARQDRSLSWI
                     VQKAWKIARRDILKYPSISDPAGDGTDS"
     gene            1609..2088
                     /locus_tag="VGB85_17505"
     CDS             1609..2088
                     /locus_tag="VGB85_17505"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEY0135888.1"
                     /translation="MASPAKATLKAALLAQLEKDLATARNAHKAAVEGATHAEARPEN
                     DKDTRGLEQSYLARGVALRAAELEAATAGIAGWHVRDFASDAVIALGALVSVDDDGRV
                     RRFLLAAHGGGVTLPGDISVITPTSPIGRALLGRRVDDDCEVHLGGSLRTLSIVGLE"
     gene            complement(2089..3345)
                     /locus_tag="VGB85_17510"
     CDS             complement(2089..3345)
                     /locus_tag="VGB85_17510"
                     /inference="COORDINATES: protein motif:HMM:NF006769.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEY0135889.1"
                     /translation="MSDGGSINTLVVSGTRLWDSLMTLAKIGATDKGGVKRLALTDLD
                     QQGRELVISWAKELGMSVVVDQIGNTFMRRAGTNDALPPVMCGSHIDTQPTGGKFDGN
                     YGVLAGLEVVRTLNEHDIRTEAPIEVAFWTNEEGSRFVPVMMGSGVFASAFSLEHAWS
                     ARDVDGKSVKDELERIGFVGPQRPGDHPVGAYFEAHIEQGPVLEDADVTIGVVTGCLG
                     QRWYDCEVTGMEAHAGPTPMQLRKDALQVATRIMQETVATALRYPPYGRGTNGFVQVF
                     PNSRNVIPGRVKFSIDLRNVDDATLNKMDADIRAFVKRTAEESGLGITIEQVSYFPPC
                     PFEQGCVTAVREGAKRLGYSVMDCVSGAGHDAIYVARLAPAGMIFVPCKDGISHNEIE
                     DAKPEHLEAGCNVLLHAMLDRARVVV"
     gene            complement(3431..>4751)
                     /locus_tag="VGB85_17515"
     CDS             complement(3431..>4751)
                     /locus_tag="VGB85_17515"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0135890.1"
                     /translation="LLVVDSRDEDVVRRGQSFIALCLTFAGFTAALLVPIAIADPAGD
                     LPMSLAVLAIIICNYSLGVVMARRGHVDLAGIVVGGALSTIVALAILLRFQALNDGIW
                     FMVLSVIISGMAIRPGFIWVVLGLDLLLTTAFLVFLPADPVLPYHNFGRVMILDALLM
                     TAAVATYINATRSRDLFRRQRSAVRELEAERLRADLAREQAEQALQLAETANLAKSVF
                     LATMSHELRTPLNAIIGYSEILREEADEAARADLDRIHGAGEHLLMLISDILDLTKIE
                     AGRMEIDAEPFAVGAFVEELAQTMRPLIDRNLNTLVVEHAAGCGEAHTDRTRLRQILL
                     NLLGNAAKFTHAGRIDLRCRCEADWLVFEVADTGIGMDEETLGRVFHEFVQADASTTR
                     RYGGTGLGLVLSRRLAGMLGGSVSVTSAPGKGSTFTLRVPATYDPAA"
BASE COUNT          776 a         1677 c         1581 g          717 t
ORIGIN      
        1 accgacgaga ccgggaagac ggcgttaccc tactggctcg aatgggatac gggtcccgtg
       61 aacgcgttcg tctgggtccg cgtacccacg atcgccgccg acgacatcac gacgatccgc
      121 atgtactacg gcaacccgaa cgccgccgct ggcagcgacg gattcgccac cttcctgttc
      181 ttcgatgact tcgaggccgc gagcctcgac gcggccaagt ggaagacgac cgctccggtg
      241 tcgatcaact tcggccgtct gtcggtcact gccggctcgg tttactcggt caagccggtg
      301 gcgagcttcc cggacacgtg ggccgaggcc cgcatggtct ggaagcccga gatcggcaac
      361 ggatacccca gcggcctgat caccagccag ggacaggcgc cgggacctgg gatccaatac
      421 ttcaaccgtc aggccaacgc ggcgatcgtg ttcgacggca aaaagttcct ggcagaaaag
      481 atggtgcagt ccgccaacgg cgaaaatccg gtgatcatgg ggatcgccgc cgacgacacc
      541 gatacctatt tctcctacaa tcgtgggttt ccggagagcc cctcggacca gctcgccttc
      601 acctactacc tgctgttcgg ccacccgtac ggcaagagcg gtgatatgac caacgtgggc
      661 gatctcgacg tcgactgggt cctcgtccgg cgtcacgccg acatggatcc aaccaccgcc
      721 gtcggcggcg aaaagacgcc gtgaccgcgc gccgcggtcc ggtcggtaat tacccggagg
      781 tctgttcggc gcgccgctgg agcgtgcgat tcatcgcctc gaacgccggc cgtgcgtcgc
      841 gttcgagggt gcgggcgagc agcgggacca gcagcccgct gtagacctcc ccctgcatga
      901 acttgacctg ctgcggtccg agcggctcga tcgagaacca gtgctcgccg gagaacacga
      961 ggcgatggcc gatgacgccg cgccagcgca gcatgtaggg ggcctccaca cgctgcacga
     1021 ccggggcgaa gcgcatctcg cggccggcga cgacccggag ccggagcgtg gagccggggt
     1081 ggagctcgcc ggtcgcctcg ggcgtgagcg ggttccactc cgggtagcga gcgaagtcga
     1141 cgaggatgtc ccagacgcgc ccggcggagg ccgcgatcac cagttcggtc cggatctcct
     1201 tcatcgtcga agggtaccgt cacgcccccc ccgggtgcac tcctcgctgg aacggcgtgc
     1261 caggcacaac cctgatcctg tagacttcgg ggtcatgacc accccacctg gaccccagga
     1321 tcccgcaacc tcggacaagc gcaagcagtc cctctacttc cctgagcaga tgctcaagga
     1381 gatccaggag gaggccgcgc ggcaggatcg gtcgctctca tggatcgtcc agaaggcgtg
     1441 gaagatcgcc cgcagggaca tcctgaagta ccccagcatc agcgaccccg ccggcgacgg
     1501 caccgactcc taaccgactc ctgaccggct cctgaccgac tcctgacctg tttgcccgcc
     1561 cgggccggct cctggtcggg cgggggttgg atggcctacc atcggcgcgt ggcctcgccc
     1621 gcgaaagcaa ccctcaaggc cgccctgctc gcgcagctcg agaaggacct cgccaccgcg
     1681 aggaacgctc acaaggccgc cgtcgagggc gccacgcacg ccgaggcgcg acccgagaac
     1741 gacaaggaca cccgcggcct cgagcagtcg tacctcgcgc gcggcgtggc cctgcgggcg
     1801 gccgagctcg aggccgcgac cgccgggatc gccggctggc acgtgcgcga ttttgcgagc
     1861 gacgccgtga tcgccctcgg tgcgctggtc tcggtcgacg acgacggccg cgtgcgccgc
     1921 ttcctgctcg ccgcgcacgg cggcggcgtc accctgcccg gcgacatctc ggtcatcacg
     1981 ccgacctcac cgatcggccg cgcattgctc ggccggcgcg tcgacgacga ctgcgaggtc
     2041 cacctcggcg gcagcctgcg cacgctgagc atcgtcgggc tcgagtgatc agaccacgac
     2101 gcgggcgcga tcgagcatcg cgtgcagcag cacgttgcag ccggcctcga ggtgctcggg
     2161 cttggcgtcc tcgatctcgt tgtggctgat gccgtccttg cacggcacga agatcatgcc
     2221 cgccggcgcc aaccgggcga cgtagatggc atcgtggccg gcccccgaga cgcagtccat
     2281 caccgagtag ccgagccgct tcgcgccctc gcgcacggcg gtcacgcagc cctgctcgaa
     2341 cgggcacggc gggaagtagg agacctgctc gatcgtgatc ccgagcccgc tctcctcggc
     2401 ggtccgcttc acgaacgcgc ggatgtcggc gtccatcttg ttgagcgtcg cgtcgtcgac
     2461 gttgcgcagg tcgatcgaga acttcacgcg gcccgggatc acgttcctgc tgttcgggaa
     2521 cacctgcacg aagccgttgg tgccgcggcc gtagggcggg tagcgcagcg ccgtcgcgac
     2581 cgtctcctgc atgatcctcg tcgcgacctg cagcgcgtcc ttgcgcagct gcatcggcgt
     2641 cgggccggcg tgcgcctcca tgccggtgac ctcgcagtcg taccagcgct ggcccaggca
     2701 gccggtcacg acgccgatcg tgacatcggc gtcctcgagc accgggccct gctcgatgtg
     2761 cgcctcgaag tacgcgccga ccgggtgatc gccgggccgc tgggggccga caaagccgat
     2821 ccgctcgagc tcgtccttga ccgacttgcc gtcgacgtcc ctcgcgctcc atgcgtgctc
     2881 gaggctaaac gcgctggcga acacgccgct gcccatcatg accggcacga agcgcgagcc
     2941 ctcctcgttg gtccaaaatg cgacctcgat cggggcctcg gtgcggatgt cgtgctcgtt
     3001 gagcgtgcgg accacctcga gaccggcgag cacgccgtag ttgccgtcga acttgccgcc
     3061 ggtcggctgg gtgtcgatgt gactgccgca catcaccggc ggcagggcgt cgttggtgcc
     3121 cgcgcggcgc atgaaggtgt tgccgatctg gtcgacgacc acgctcatgc cgagctcctt
     3181 cgcccacgag atcacgagct cgcgcccctg ctggtcgagg tcggtcagcg ccagccgctt
     3241 cacgccgccc ttgtcggtgg cgccgatctt cgcgagggtc atcagcgagt cccacagccg
     3301 ggtgccgctg acgacgaggg tgtttatcga tccgccgtcg ctcatgcacg ctcaatatag
     3361 ccccgcaagc ggcccccgcg aaagcccaaa tcgccgggcc gacaccagcg ggtggactaa
     3421 ccgaggcgcc tcacgccgcc gggtcgtacg tcgcgggcac gcgcagcgtg aaggtcgagc
     3481 ccttgccggg cgcgctggtg acggacaccg acccgccgag catcccggcg agcctgcgcg
     3541 acagcaccag gccgaggccc gtgccgccat agcgtcgcgt cgtcgaggcg tcggcctgga
     3601 cgaactcgtg gaacacccgc ccgagggtct cctcgtccat gccgatcccc gtgtcggcga
     3661 cctcgaacac cagccagtcg gcctcacagc ggcagcgcag gtcgatcctg cccgcgtgcg
     3721 tgaacttggc cgcgttacca agcaagttga gcaagatctg gcgcaggcgc gtgcggtcgg
     3781 tgtgggcctc gccgcagccc gcggcgtgct cgacgacgag cgtgttgaga ttcctgtcga
     3841 tcagcggtcg catcgtctgc gcgagctcct cgacgaaggc gccgacagcg aacggctcgg
     3901 cgtcgatctc catgcgcccg gcctcgatct tggtgaggtc gaggatatcg ctgatcagca
     3961 tcaggaggtg ctcgccggcg ccgtggatcc ggtcgaggtc cgcgcgcgcg gcctcgtccg
     4021 cctcctcgcg caggatctcc gagtacccga tgatcgcgtt gagcggggtg cgaagctcgt
     4081 gcgacatcgt cgccaaaaac acgctcttgg ccaggttcgc ggtctcggcc agctgcagcg
     4141 cctgctcggc ctgctcgcgc gccaggtcgg cgcgcaggcg ctcggcctcg agctcgcgca
     4201 cggcgctgcg ctggcgccgg aacaggtcgc ggctgcgcgt cgcgttgatg tatgtcgcga
     4261 ccgccgcggt catcagcagc gcgtccaata tcatgaccct gccaaagtta tggtacggca
     4321 gcacggggtc ggccgggagg aacacgagga aggcggtcgt cagcagcagg tcgaggccga
     4381 gcaccaccca tataaaaccc gggcggatcg ccatgccgga gatgatcacg ctgaggacca
     4441 tgaaccagat gccgtcattg agcgcctgga agcgcagcag gatcgccagc gcgacgatcg
     4501 tgctcagcgc cccgccgacg acgatgcccg ccaggtcgac gtgaccccgg cgcgccatca
     4561 ccacgcccaa agaatagttg cagatgatga tcgctagcac ggcgaggctc atcggcaggt
     4621 cgcccgccgg gtccgcgatc gcgatcggca ccagcagcgc cgcggtgaag ccggcgaagg
     4681 tgagacacag cgcgatgaac gactggccgc gccgcacgac gtcctcgtcg cggctgtcga
     4741 cgacgagcag c
//
LOCUS       DASWNJ010001037         2688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_331191, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001037 DASWNJ010000000
VERSION     DASWNJ010001037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331191"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VGB85_17520"
     CDS             <1..195
                     /locus_tag="VGB85_17520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135891.1"
                     /translation="TFGLTYPNVHDGRNTIGPLFEIVGYPTSFLIDRQGAIVWRRDGI
                     LEPGDAELARVLADALAGPA"
     gene            284..859
                     /locus_tag="VGB85_17525"
     CDS             284..859
                     /locus_tag="VGB85_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HEY0135892.1"
                     /translation="MRTYHVIYAGPGMSGRQESLRVLCSPGSPRVPEDPRYPWEHLAD
                     GADIPLASRVARVRIALMYFGNHWFPTVSAVLGSPYGRFVERLIEFLPVLDGVVFVAD
                     SQPERTEAGLAALELLLEFMRERGLDPAQVPFVFQLNKRDLPNVLHVEELRTILSTPR
                     CAHVESIAVRQRGVHQALETLLRLVDSATPP"
     gene            complement(991..1527)
                     /locus_tag="VGB85_17530"
     CDS             complement(991..1527)
                     /locus_tag="VGB85_17530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135893.1"
                     /translation="MTRVLDDAHIPMMFCGSIASTYYGMPRTTQDVDLVVDLRDTDIP
                     RLLAAFPEQEYYVSEDAVREAVQRRSQFNIIDFRTGWKADLIVRRARPFSVEELMRRQ
                     RVAILGVEVWLATAEDTVLAKLEWAALSDSERQERDVTAILRIRGAKLDWAYMERWAD
                     VLGVRQELLRLQQTATDK"
     gene            complement(1551..1805)
                     /locus_tag="VGB85_17535"
     CDS             complement(1551..1805)
                     /locus_tag="VGB85_17535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135894.1"
                     /translation="MPPATDTTPEADAVQFAVYRRMSDARRLELTLQMSDEARRITAA
                     GIRAHHPEYTDAHVEDALRLMILGPRLFHMAWPDKPLLSP"
     gene            complement(1973..>2688)
                     /locus_tag="VGB85_17540"
     CDS             complement(1973..>2688)
                     /locus_tag="VGB85_17540"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0135895.1"
                     /translation="DLETHKFAAMRAAVLAFVEEMSPRGLGYADLRALVQALRGAVLA
                     VPAAAGQRSEVEAWFFELAMVGTMQFVAYRETRLQEQAARVEVQHLESQLDEIKAAYA
                     EKTDLLERIRQSSTPVAPVVEGILVVPLVGLFDTMRAELLTDRLLQEITRARASVVIL
                     DISGVPSFDVQAAELIIRLGGAVRLLGAQLILVGVSPATASTMVEMGVVLTGLRAMGS
                     LQDGLALALRLRRLKIAPL"
BASE COUNT          407 a          965 c          886 g          430 t
ORIGIN      
        1 accttcggcc tcacctaccc gaacgtgcac gacggccgca acacgatcgg gcccctcttt
       61 gaaattgtcg gctacccgac cagcttcctg atcgaccgcc agggggcgat cgtgtggcgc
      121 agggacggga tcctcgagcc cggggacgcc gagctcgctc gcgtgctcgc cgacgccctc
      181 gccggcccgg cttgatcgtg tgggctggtt cggtcccgcg agttgctccg cacccgaatg
      241 ccgctgcgca tcgatccgag ctcatgcgtg gcatgatggt cgggtgcgga cataccacgt
      301 catctacgcc gggccgggca tgtccgggcg tcaggagtcg ctgcgcgtgc tctgctcgcc
      361 cgggtcaccg cgagtcccgg aggacccgcg atatccatgg gagcacctcg cggacggagc
      421 cgacatccca ctcgcctcca gggtcgcgcg ggtgcggatc gccctgatgt acttcgggaa
      481 ccactggttc ccgaccgtga gcgccgtgct ggggtctccg tacgggcgct tcgtcgagcg
      541 cctgatcgaa ttcttgccgg tcctcgacgg cgtcgtcttc gtcgccgact cgcaaccaga
      601 acggaccgag gccggtctcg cggcgctcga gctcctgctc gagttcatgc gcgagcgggg
      661 cctcgacccg gcccaggtcc ccttcgtgtt ccagctcaac aagcgcgacc tcccgaacgt
      721 gctccacgtc gaggagcttc ggaccatcct ctcgaccccc cgctgtgccc acgtggagtc
      781 gatcgccgtg cgccagcgag gcgtccacca ggcgctcgag accctgctcc ggctcgtcga
      841 cagcgcaacc ccgccttgat gccggatgga cggcgacgcg aacgtggcag cccgccccgc
      901 atctgcgcga gcgccgaccc atcttcgctt cgcaccgctc catcagcgga gcgtcgccgc
      961 gcggtccccg ggagcgggca aggtgagtcg ctacttgtct gtcgccgttt gctggaggcg
     1021 cagtaattct tggcggactc caagtacgtc tgcccaccgt tccatgtagg cccaatcgag
     1081 cttcgccccc cggatccgta ggatcgccgt aacatcccgc tcctgacgct ccgagtccga
     1141 gagcgccgcc cactcgagtt tggcgagcac cgtgtcctcg gccgtcgcca gccacacctc
     1201 gaccccgagg atcgccacgc gctggcgtcg catcagctcc tcgacgctaa aaggtcgagc
     1261 cctgcgtacg ataaggtccg ccttccagcc ggtgcggaaa tcgatgatgt tgaactggct
     1321 cctgcgctgc acagcctcgc gtacggcgtc ctcgctcacg tagtactctt gctccggaaa
     1381 agcagcgagt aggcgcggga tgtcggtgtc gcgcaggtcc acgacgaggt ccacgtcctg
     1441 cgtagttctt ggcatcccat agtacgtgct ggcgatggac ccacagaaca tcatcgggat
     1501 gtgggcatcg tcgaggacgc gggtcaaccg cgcgaggagc tcactgacac tcatgggctg
     1561 agcagcggct tgtccggcca tgccatgtga aagagacgcg gaccgaggat catcagccgc
     1621 agggcgtcct ccacatgtgc gtcggtgtac tcgggatggt gcgcgcggat ccccgcggcc
     1681 gtgatgcggc gcgcttcgtc ggacatctgc agcgtaagtt caaggcgtcg cgcgtcgctc
     1741 atccggcggt agacggcgaa ttggacggca tcggcctccg gcgtcgtgtc tgtggcgggt
     1801 ggcatggctg gccgcactat atccggggcc tcgcgtgcgc gccattcatg ggcgagcaca
     1861 agtggggcaa cgctcgatgg agccatgctc ggcaacggcg tcaccgtcgc ggtcggcccg
     1921 cgcaagtgct tcgccctcgc cggcccggcg tgacggccgg gcgagccgcc ggtcacagcg
     1981 gcgcgatctt caggcgtcgc agccgcaacg ccagcgccag gccgtcctgc agcgagccca
     2041 tcgcccgcag tccagtgagc acgacgccca tctcgaccat cgtgctcgcg gtcgccggcg
     2101 agacgccgac gaggatcagc tgggccccga gcaggcgcac cgcgccgccg aggcgaatga
     2161 tcagctcggc cgcctggaca tcgaacgagg gcacgccgga gatgtcgagg atcaccaccg
     2221 aggcgcgcgc acgcgtgatc tcctgcagca gccggtccgt caacagctcg gcgcgcatgg
     2281 tgtcgaacag gccgaccagc ggcacgacga ggatgccctc gacgaccggg gcgaccggcg
     2341 tcgagctctg ccggatccgc tcgagcaggt cggtcttctc cgcgtacgcg gccttgatct
     2401 cgtcgagctg cgactcgagg tgctggacct cgacccgggc cgcctgctcc tgcaggcgcg
     2461 tctcgcggta cgcgacgaac tgcatcgtgc cgaccatcgc cagctcgaag aaccacgcct
     2521 cgacctcgct gcgctgcccg gcggccgcgg gcaccgcgag caccgcgccg cgcagcgcct
     2581 gcaccagcgc gcgcaggtcg gcgtagccga ggccgcgcgg gctcatctcc tcgacgaacg
     2641 cgagcacggc ggcgcgcatc gccgcaaact tgtgggtctc gaggtcct
//
LOCUS       DASWNJ010001038         1547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_584139, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001038 DASWNJ010000000
VERSION     DASWNJ010001038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584139"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1547
                     /locus_tag="VGB85_17545"
     CDS             <1..>1547
                     /locus_tag="VGB85_17545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEY0135896.1"
                     /translation="ALGLKAKIVAENVTSRDALTRANQMREVVERIGQLGDAERVSVM
                     SRLFGKETLGTIGPLATNLELLTESLELAGDKTKALGSVQAEFQSRSATTANTLQLLK
                     NSVSVTAITIGNELLPEISVMAKDLAGWVKQNRELIKDNVVGFVHGLRDAVKGIAPYV
                     AGTAKLLGTVVEMLGGVERAIGPVTVGLGALRVASTLALGPWGVLAAGIIAGAVAIKQ
                     EMADAERRTMSLIQAAGRLKDVRMTGFGLETKSAQELLKMQEDLTAERKKAATISGTS
                     KSGRFLSPSELAQAEKERQADLAAIDERSKLIRDALSTKTKELETSINIRKESERAAA
                     AEQLRSDKLKLELEYLNSLDKLSAAQARRKKALEKVGVEAEEPAAKKGRGGRGPKKTG
                     GEMLDPGDVRIARTFDGTGQTEEVASGAVEAAAAGAAEAEQRRFQEQMLRFEREQEML
                     DASGLREEEAELRRADLQNRRMAREEEYAKFQLNSATDEASREAARTKLETVEHNKRV
                     AGLKNQV"
BASE COUNT          304 a          478 c          556 g          209 t
ORIGIN      
        1 aggcgctggg gctcaaggca aagatcgtcg ccgagaacgt gacctctcgt gacgccctga
       61 cccgcgcgaa ccagatgcgc gaggtcgtgg agcgcatcgg ccagctcggc gacgcggagc
      121 gcgtcagtgt gatgtcccgg ctcttcggca aggaaacgct cggcaccatc ggcccgctgg
      181 cgacaaacct cgagcttctc acggagtcgc tcgagctggc cggtgacaag acgaaggcgc
      241 tgggcagtgt ccaggctgag tttcaatcgc gcagcgcgac gacggcgaac acgcttcagc
      301 ttctgaagaa cagcgtcagt gtcacggcga tcaccatcgg taacgaactg ctgcctgaga
      361 tctccgtcat ggcgaaggac ctggcagggt gggtcaagca gaaccgcgag ctgatcaagg
      421 acaacgtggt cggcttcgtc cacgggctgc gcgacgctgt gaagggcatc gccccctacg
      481 tcgccggcac ggctaagttg ctcggcaccg tggtcgagat gctcggcggc gtcgagcggg
      541 cgatcggccc ggtgaccgta gggctgggag cgctgcgtgt cgcgtcgacg ctggcgctcg
      601 gcccgtgggg cgtgctcgca gccggcatca ttgcaggtgc cgtagccatc aagcaggaga
      661 tggccgacgc ggagcggcgc acgatgtcct tgattcaggc cgcgggccga ctcaaggacg
      721 tgcggatgac cggcttcggg ctcgagacca agtcggcgca ggaactgctc aagatgcagg
      781 aggacctgac cgcggagcgc aagaaggccg cgacgatcag cggcacgtcg aagtccggtc
      841 gcttcctgtc gccgagcgag ctggcccagg cagagaagga gcggcaggcc gacctcgccg
      901 cgatcgatga gcgctcgaag ctcatccgcg atgcgctgtc gacgaagacg aaggagctgg
      961 agacctcgat caacatccgc aaggagtctg agcgagcggc cgccgccgag cagctccgct
     1021 cggacaagtt gaagctcgag ctggagtacc tcaacagcct cgacaagctc agcgcagcgc
     1081 aggctcgccg caagaaggcg ctcgagaaag tcggcgtcga agctgaggag cccgcggcga
     1141 agaagggtcg cggcggtcgc ggccccaaga agaccggcgg cgagatgctc gacccgggcg
     1201 acgttcgcat cgcgcgcacc ttcgacggga ccggccaaac cgaggaggtt gccagcggtg
     1261 cggtcgaggc ggccgcggct ggtgcggccg aagccgagca gcgccgcttt caggagcaga
     1321 tgcttcggtt cgagcgcgag caggagatgc tcgatgcgtc gggcctgcgc gaggaggaag
     1381 cagagctacg ccgtgcagat ctccagaacc gccggatggc gcgcgaggaa gagtacgcca
     1441 agttccagct caactccgcg accgatgagg cgtcgcgcga ggctgcccgg accaagctcg
     1501 agaccgtcga gcacaacaag cgcgtcgctg gcctgaagaa tcaggtt
//
LOCUS       DASWNJ010001039         3539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_302294, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001039 DASWNJ010000000
VERSION     DASWNJ010001039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302294"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VGB85_17550"
     CDS             <1..269
                     /locus_tag="VGB85_17550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135897.1"
                     /translation="NAPISVQGRPTASQVEKGADPRAVITFLGRPQTDDDDAHLEGIV
                     IMRDFLLDEINEDDDIPELMIVSIIDELRRFFRMEGLEMGTGEE"
     gene            281..1531
                     /locus_tag="VGB85_17555"
     CDS             281..1531
                     /locus_tag="VGB85_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY0135898.1"
                     /translation="MATHANTAALIYDWNEKERTGPLLRRAPRFVDESIRDGIQSPSV
                     RDPSIEQKMQLVRLMDRMGIQTVNLGLPGAGKRAQDDVERLLRFIVEEKLRITTNCAA
                     RTMASDITPIIDISQRVGVPIEVMTFIGSSPIRSYAEGWDNDRLKKHTLDAVKLAVDN
                     GLPVGFVTEDTTRSHPKTLDILFRTAIDAGASRLVLCDTCGHATPDGLRNLLNFTRGV
                     LRSMDVEDRIELDWHGHNDRGLALVLGLYAFEWGVDRVHGCGLGIGERVGNTSMDLLL
                     LNLYLLGQLDAKRHDLSCLVEYVEAVSNYTGVPIHPSYPLSGRDAFRTATGVHAAAVI
                     KAEKTGDHDLSDRVYSAVPARVFGRKQEIEIGHYSGRSNVLYWLKSHGYEPSDELVDR
                     VFNFAKLQDHTLTTDEVKAVIAQA"
     gene            complement(1583..3163)
                     /locus_tag="VGB85_17560"
     CDS             complement(1583..3163)
                     /locus_tag="VGB85_17560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135899.1"
                     /translation="MRRQAPIKRSPGTTGQRTVAGTKAADATSLDDRPLAIDLSRDGK
                     HLIVALPYEVWLLSNATLEVERTIELTSPEPSVTEASKDGALWIGGHHLHYGSIFSST
                     VTKVGTKLADFVDRVCLLRPGLLCGVGHHGEVLWDIEKETPVHRRKSPEHPVLGLVAL
                     EGRAVWADGSQNAWVIDPQRPEGYMQLKLRTTSTTEVEAEGIVALGLTSAGRCILAAR
                     DGAVAWTGRHMRVDAEKFPRLPLRAATALAVAGDERWVYVLRPRGLLQRFIIEQPKPA
                     AGEEDDFVPLPEAEECRLEWSAECMLLSGSAAGAPRLVFGGPQADGTLGRLWRVDPGA
                     LSWQPAKLGKRTLVEPKPAEAVDKRPDFTLTRSKLTGPGLGELKVDAVLGADAQAVFV
                     THGHGALLERPVLRRPAAEVLPADALLLPVMIRSREGTARPGLVLWPGTHNPDREPPE
                     LQWLVWGDQPRGWMPLETPQIRAQGWSRTDLFPMQIALSAVPTVAGNRAAIPEKWVDP
                     ELFQALARECKKLLKVLW"
     gene            complement(3189..>3539)
                     /locus_tag="VGB85_17565"
     CDS             complement(3189..>3539)
                     /locus_tag="VGB85_17565"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975356.1"
                     /note="Catalyzes the oxidation of dihydrolipoamide to
                     lipoamide; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY0135900.1"
                     /translation="IYTHPAIASVGKTEAELKQAGIPYNKGSFPFAANGRAKALGDTD
                     GFVKILAHKETDRILGVHIIGPRASDLIAEAAVAVEFSASAEDLARSVHAHPTLSEVV
                     KEAALDAGGRVIHF"
BASE COUNT          538 a         1200 c         1271 g          530 t
ORIGIN      
        1 cgaacgcgcc gatctccgtg caggggcgcc cgacggcctc gcaggtcgag aagggtgcag
       61 atccgcgcgc cgtgatcacc ttcctgggcc gcccgcagac cgacgacgat gacgcgcacc
      121 tcgagggcat cgtcatcatg cgcgacttcc tcctcgacga gatcaacgag gacgacgaca
      181 tccccgagct gatgatcgtg agcatcatcg acgagctgcg ccgcttcttc cgcatggaag
      241 gcctggagat gggcaccggg gaggagtaaa ccaaggtccc atggcgactc acgcgaacac
      301 tgccgccctc atctacgact ggaacgagaa ggagcgtacc ggaccgctgt tgcggcgggc
      361 cccgcgcttc gtcgacgaga gcatccgcga cggcatccag tcgccgtcgg tgcgcgaccc
      421 gtcgatcgaa cagaagatgc agctcgtgcg gctgatggat cgcatgggga tccagaccgt
      481 caacctgggc ctgcccggcg ctggcaagcg ggcccaggac gatgtcgagc ggctgctgcg
      541 cttcatcgtc gaggagaagc tgcgcatcac caccaactgc gcggcgcgga cgatggcctc
      601 ggacatcacg ccgatcatcg acatctcgca gcgggtcggc gtgccgatcg aggtgatgac
      661 cttcatcggc tcgagcccga tccgctcgta cgccgagggc tgggacaacg accggctcaa
      721 gaagcacacg ctcgacgcgg tcaagctggc ggtcgacaac ggcctgccgg tcggcttcgt
      781 caccgaggac acgacgcgct cgcacccgaa gacgctcgac atcctgtttc gtacggcgat
      841 cgacgcgggg gcctcgcggc tggtgctgtg cgacacctgc gggcacgcga cgcccgatgg
      901 tctacgcaac ctcctcaact tcacgcgcgg agtgctccgc tcgatggacg tcgaggaccg
      961 gatcgagctc gactggcacg gccacaacga ccgcgggctg gcgctggtgc tcgggctgta
     1021 cgcgttcgag tggggcgtcg accgggtgca cggctgcggc ctcggcatcg gcgagcgggt
     1081 cggcaacacc tcgatggacc tgctgctgct caacctgtac ctgctcggcc agctcgacgc
     1141 gaagcgccac gacctgtcgt gcctggtcga gtacgtcgag gcggtcagca actacacggg
     1201 cgtgccgatc cacccgagct acccgctgtc gggccgcgac gcgttccgca cggcgacggg
     1261 cgtgcacgcg gcggcggtga tcaaggccga gaagacgggg gaccacgacc tctcggaccg
     1321 cgtgtactcg gcggtgccgg cccgcgtgtt cgggcgcaag caggagatcg agatcgggca
     1381 ctacagcggc cgctcgaacg tcctgtactg gctcaagtcc cacggctacg agccgtcgga
     1441 cgagctcgtc gatcgcgtgt tcaacttcgc caagctgcag gatcacacgc tgacgaccga
     1501 cgaggtcaag gctgtgatcg ctcaggcttg agttgggctg acggaaggga caggtccgat
     1561 cggacctgcc cctttattaa tttcaccaga ggaccttgag cagcttcttg cactcgcggg
     1621 cgagcgcctg gaacagctcg gggtcgaccc acttctcggg gatcgcagcg cggttgcccg
     1681 cgacggtcgg gaccgcggag agggcgatct gcatcgggaa caggtcggtg cgggaccagc
     1741 cctgggcgcg aatttgcggg gtctcgagcg gcatccagcc gcgcggctga tcgccccaga
     1801 ccagccattg cagctcgggc ggctcgcggt ccgggttgtg cgtgccgggc cacagcacca
     1861 ggccgggtct ggccgtgccc tcgcgcgagc ggatcatcac cggcaagagc agggcgtcgg
     1921 ccggcaagac ctcggccgcg gggcggcgga gcaccggccg ttcgagcaag gcgccgtggc
     1981 cgtgggtcac gaacacggcc tgcgcgtcgg cgccgagcac ggcgtcgacc ttgagctcgc
     2041 cgaggccggg gccggtcagc ttgctccgcg tcagggtgaa gtccggccgc ttgtcgaccg
     2101 cctcggccgg cttcggctcg acgagcgtgc gcttgccgag cttggccggt tgccagctca
     2161 gggcgcccgg gtcgactcgc cacaagcggc cgagggtgcc gtcggcctgc ggcccgccga
     2221 acaccagccg cggcgcgccg gcagccgagc cggacagcag catgcactcg gccgaccact
     2281 cgaggcggca ctcctccgcc tccggcaagg gcacgaagtc gtcctcctca ccggcggccg
     2341 gcttcggctg ctcgatgatg aagcgctgca agaggccgcg ggggcgcagg acgtagaccc
     2401 agcgctcgtc accggcgacc gccagcgcgg tggcggcgcg cagcggcagg cgggggaact
     2461 tctcggcgtc gacgcgcatg tgtcggccgg tccaggcgac ggcgccgtcg cgggcggcga
     2521 ggatgcagcg gccggcgctg gtcaggccga gcgcgacgat gccctcggcc tcgacctcgg
     2581 tggtgctcgt cgtgcgcagc ttgagctgca tgtagccctc gggtcgctgc gggtcgatga
     2641 cccacgcgtt ttgcgagccg tcggcccaca cggcgcggcc ctcgagcgcg accaggccga
     2701 gcaccgggtg ctcgggcgac ttgcggcgat ggacgggcgt ctccttttcg atgtcccaca
     2761 ggacctcgcc gtggtggccg acgccgcaca gcagtccggg gcgcagcagg cacacgcggt
     2821 cgacgaagtc ggcgagcttg gtgccgacct tggtgaccgt ggaggagaag atgctgccgt
     2881 agtggaggtg atgcccaccg atccacagcg cgccgtcctt gctggcctcg gtgacgctcg
     2941 gctcggggga cgtgagctcg atcgtccgct cgacctccag cgtggcgttg ctgagcagcc
     3001 acacctcgta cggcagcgcg acgatcaggt gcttgccatc gcggctgagg tcgatcgcca
     3061 gcggccggtc gtccagcgag gtggcgtcgg cggccttggt gccggcgacg gttctctggc
     3121 ccgtcgtgcc cggggatcgc ttgatgggag cctgccgccg catgggctgc gagcgtatca
     3181 cgccacccct agaagtggat cacgcggccg ccggcgtcga gggcggcctc cttgacgacc
     3241 tcggagaggg tcggatgcgc gtgcacgctg cgggccagat cctcggcgct ggccgagaac
     3301 tccacggcga cggccgcctc ggcgatcagg tcgctcgccc gggggccgat gatgtgcacg
     3361 ccgaggatcc ggtcggtctc cttgtgagcc aggatcttca cgaagccgtc ggtgtccccg
     3421 agcgccttgg cccggccgtt ggcggcgaac gggaagctgc ccttgttgta cgggatcccc
     3481 gcctgcttga gctcggcctc ggtcttgccg acgctggcga tcgccgggtg cgtgtaaat
//
LOCUS       DASWNJ010001040         1650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_19421, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001040 DASWNJ010000000
VERSION     DASWNJ010001040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19421"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1457
                     /gene="purB"
                     /locus_tag="VGB85_17570"
     CDS             <1..1457
                     /gene="purB"
                     /locus_tag="VGB85_17570"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEY0135901.1"
                     /translation="EYLAPAMTAPQPGYTSPLGQRYATPAMLENFSDRRRVLLWRDLW
                     IALAQAERDLGLPISQEQVDALRAAREEIDLARVAELEAKLRHDVMAHVHHFGEVAGA
                     DAAKIIHLGATSCFVTDNAELVMIRHGLELVMDRLVAAIEQLAAFAEEHRGLACLAFT
                     HYQPAQLTTVGKRACLWAQDLALDLETIEALVSRLPLRGVKGTTGTQASFLGLFAGDH
                     DKVRALDRRVCEAMGFVGSIAVSGQTYTRKLDTWVVSALADVCGSSAKLATDLRLLAH
                     EREVDEPFEAHQVGSSAMAYKRNPMRSERIVSLARFVHSLAGSPRETHANQWFERTLD
                     DSANRRLVITEAFLATDAVLNLLVDVTGGLIVHPAVIRANMRDELPFMATENVIMRGA
                     AAGESRQHLHEAVRGASLAAVARMKQGEANDLVARMQADPALARFVDPGLIDPERYVG
                     RAPEQVDEYLAESLRPLLARHAHRRGRFVAGVRV"
BASE COUNT          243 a          596 c          581 g          230 t
ORIGIN      
        1 gcgagtatct tgcgcccgcg atgaccgcgc cacaacccgg ctacaccagc cccctgggcc
       61 agcgctacgc gaccccggcg atgctcgaga acttctcgga ccgccgccgg gtgctgctgt
      121 ggcgagatct atggatcgcg ctggcgcagg ccgagcggga cctcgggctg ccgatctcac
      181 aagagcaggt cgacgcgctg cgggccgcca gggaggagat cgatctcgcc agggtggccg
      241 agctcgaggc caagctgcgc cacgacgtga tggcccacgt gcatcacttc ggcgaggtcg
      301 ccggcgccga cgcggcgaag atcattcacc tcggggcgac gagctgcttc gtgaccgaca
      361 acgcggagct ggtgatgatc cgccacggcc tcgagctggt gatggaccgc ctggtcgccg
      421 cgatcgagca gctggcggcg ttcgccgagg agcaccgcgg cctcgcgtgc ctggccttca
      481 cgcactacca gccggcccag ctgacgacgg tcggcaagcg cgcgtgcctg tgggcgcagg
      541 acctggccct cgacctcgag acgatcgagg cgctggtgtc ccgcctgccg ctgcgcggcg
      601 tcaagggcac gaccggcacg caggcgtcgt tcctcggcct gttcgccggc gaccacgaca
      661 aggtccgcgc gctcgaccgc cgcgtctgcg aggcgatggg ctttgtcgga tcgatcgcgg
      721 tctcgggcca gacctacacg cgaaagctcg acacctgggt ggtctcggcg ctcgccgacg
      781 tgtgcggatc ttcggccaag ctggcgacgg acttacgcct gctggcccac gagcgcgaag
      841 tggacgagcc cttcgaggcc caccaggtcg ggtcgtcggc gatggcctac aagcgcaacc
      901 cgatgcgcag cgagcggatc gtcagcctgg cccgcttcgt ccactcgctt gccggcagcc
      961 cccgcgagac ccacgcgaat cagtggttcg agcgcaccct cgatgacagc gcgaaccgcc
     1021 gcctggtgat cacggaggcg ttcctggcga ccgacgcggt gctcaacctg ctcgtcgacg
     1081 tcaccggcgg cctcatcgtc cacccggcgg tgatccgcgc caacatgcgc gacgagctgc
     1141 cgttcatggc gaccgagaac gtgatcatgc gcggggccgc ggccggcgag agccggcagc
     1201 acctgcacga ggcggtccgc ggcgccagcc tggcggcggt cgcccgcatg aagcaggggg
     1261 aggccaacga cctcgtcgcc cgcatgcagg ccgacccggc cctcgctcgc ttcgtcgacc
     1321 ctggcctcat cgatcccgag cgttacgtcg gtcgcgcgcc cgagcaggtc gacgagtacc
     1381 tcgccgagag cctgcgcccg ctgctcgctc gccacgcgca tcgccgcggg cgcttcgtcg
     1441 ctggcgtccg ggtgtgatcc gatgcggtct gagcctgtcc cgaaggacag gcgtcagcga
     1501 gggcgccgtc gcagcgcgcc gagcagcacg agcagcgcca gcgatccgaa catgccggag
     1561 cgcgggtcgc tggcgcagcc gcagccgtcg ctgcgctccg ggtcggtgtt gatgcagtcg
     1621 cacgcctcgc agtcggcggg gcgaagatcg
//
LOCUS       DASWNJ010001041         2990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_655004, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001041 DASWNJ010000000
VERSION     DASWNJ010001041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655004"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VGB85_17575"
     CDS             complement(<1..237)
                     /locus_tag="VGB85_17575"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEY0135902.1"
                     /translation="MGDRKNDLTLAEVSTPLPTLHRTLGPGMEGHDVARLQAGLRRLG
                     YVVGPIDGIFGDRTASAIAEYCTKRKWPGPAVADA"
     gene            complement(292..1371)
                     /locus_tag="VGB85_17580"
     CDS             complement(292..1371)
                     /locus_tag="VGB85_17580"
                     /EC_number="3.1.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF027980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ca2+-dependent phosphoinositide-specific
                     phospholipase C"
                     /protein_id="HEY0135903.1"
                     /translation="MRTHHVVLGSLALLFACASSPPEATTGTTGTMGTMGEASTAAET
                     GTPTTGSAESSTGAPPEQGLRLHQLQAMGTHNSYHISSGESVKDLDYTHKPLPEQLDA
                     GARQFELDINFSKMAGEAIGVYHLELLDQKSNCPLLKDCLQGLRGWSEGHPGHHILYV
                     MFEVKTAFNAVLWDEFIADLEAEILAVWPREDIVAPDDVQGDAASIREGLAANGWPTI
                     AASRGKILFVLHDGGNWRDSYVAAGTHGHLLFPDAFGDLAAPYAAVHTLNDPTGDAAA
                     IAAALDAGHLVRTRADADLVEPLAGDDTRAMAALASGATFISTDYPPPMGEVDYVFEI
                     PEGTPSRCNPRTAPADCTPADIEAP"
     gene            complement(1425..>2990)
                     /locus_tag="VGB85_17585"
     CDS             complement(1425..>2990)
                     /locus_tag="VGB85_17585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135904.1"
                     /translation="QPTLAPGAACDPDRVAAADTPDPEDDKDPQPICAPGLVCDPVVD
                     SDDHVCGAALQIRGRVEDSLTGLGIEAALVAALNENGEPVTDVAATDSCGDYVLPIRV
                     RRNPDGSFAESLKWTLIASARNYQPFPAGPRPALPIDLSEAVPNPDGPEPALASKDAE
                     DDADPPMTYTVEVIDTAATSVALIPLGAAAQGVSVSGSVGADAAGTLVVAEGGAAPAP
                     YAIADASGAFTLFNVQSGAVEVRGYRQGVELAPAPVTVAAADVVDVDLAIVTVDVAAL
                     GTVTGSIGIVNAPGGSMTSVVLVPSSVYNAGLERGPVPLGLRDPPPPATPDVTGQFTL
                     TGVPAGTYKVLVAFENDSLVRDPDEGISGTQIQEITLAAGQMLPVAEGFKVTEALAIV
                     GPGKDGPEAVAATPTLTWVDDSSEDGYDVVVYDALGNLVWETEVAGVSGSDNVELAYA
                     GPALVSGMYYQFRVTSWREVQNKRLNIARTEDLRGVFYTGEAPEQTACVAEDPEPTGG
                     DDTDTGGAVTTGE"
BASE COUNT          400 a         1018 c         1110 g          462 t
ORIGIN      
        1 agcgtcggcg acggccgggc cgggccactt gcgcttcgtg caatactcgg cgatcgcgct
       61 ggcggtgcga tctccgaaga taccgtcgat cggaccgacc acgtagccga ggcggcgcag
      121 cccggcttgc aggcgggcga cgtcgtggcc ctccatgccg gggccgagcg tgcggtgcag
      181 cgtcgggaga ggcgttgaga cttcagcgag ggtgaggtcg ttcttgcggt cgcccatggt
      241 ccgtcagggt aacgcgacga cgcgggcgag agcgcacccc gaggtcgtcg gtcagggcgc
      301 ctcgatgtcc gcgggcgtgc agtcggccgg ggccgtgcgc ggattacagc gtgaaggcgt
      361 accttcgggg atctcgaaca catagtcgac ctcgcccatc ggcggcggat agtccgtgct
      421 gatgaaggtc gcgccgctgg cgagcgccgc catcgcgcgg gtgtcgtcgc cggcgagcgg
      481 ctcgacgagg tcggcgtcgg cgcgcgtgcg aaccaggtgc ccggcgtcga gggccgccgc
      541 gatcgcagcg gcgtcgccgg tcgggtcgtt gagcgtgtgc accgcggcgt acggggccgc
      601 gaggtcgccg aacgcgtcgg ggaacagcag gtgcccgtgc gtcccggccg ccacgtagct
      661 gtcgcgccag ttgccgccgt cgtgcagcac gaacaagatc ttgccgcgac tggcggcgat
      721 cgtcggccag ccgttcgcgg cgaggccctc gcggatgctc gcggcgtccc cctggacgtc
      781 gtcgggcgcg acgatatcct cgcgcggcca gacggcgagg atctccgcct cgagatcggc
      841 gatgaattcg tcccacagca ccgcgttgaa ggcggtcttg acctcgaaca tgacgtataa
      901 aatgtggtgc ccgggatgcc cctccgacca cccgcgcagc ccctgcaggc aatctttcaa
      961 caacgggcaa ttactcttct gatcgagcaa ttcgaggtga tacaccccaa tcgcctcccc
     1021 cgccattttt gaaaaattga tgtcgagctc gaactgacgc gcgccggcgt cgagctgctc
     1081 cggcagtggt ttgtgggtat aatcgagatc tttgacggac tcgccggagg agatatgata
     1141 gctgttgtgc gtgcccatcg cctgcagctg atgcaggcgc aggccctgct ccggcggcgc
     1201 gcccgtgctg ctctcggcgc tgccggtcgt cggtgtgccg gtctccgcgg ccgtgctcgc
     1261 ctcgcccatg gtgcccatag tgccggtggt gccggtggtc gcctccgggg ggctcgacgc
     1321 gcaggcgaac aggagggcga gggagccgag gacgacgtgg tgggtacgca cgcctcacca
     1381 taggcgcagg acgcctcacg atgcaacgtc gccggcagcc aggtctattc gccggtcgtg
     1441 acggcgccgc cggtgtccgt gtcgtcgccg ccggtcggct cggggtcctc ggcgacgcac
     1501 gcggtctgct cgggcgcctc gccggtgtag aacacgccgc gcaggtcctc ggtgcgggcg
     1561 atgttgaggc gcttgttctg gacctcgcgc cacgaggtca cgcgaaattg atagtacatc
     1621 ccggacacca gcgccggacc ggcatatgcg agctcgacgt tatcgctgcc cgacacgccg
     1681 gcgacctcgg tctcccacac gaggttgccg agggcgtcat aaacgacgac gtcgtagccg
     1741 tcctcgctcg agtcgtcgac ccacgtcagg gtgggcgtcg ccgcgacggc ctcgggcccg
     1801 tccttgccgg gcccgacgat cgccagcgcc tcggtgacct tgaagccctc cgcgactggc
     1861 agcatctgcc cggccgccag cgtgatctcc tggatctgcg tgcccgagat gccctcgtcg
     1921 gggtcgcgga ccagggagtc gttctcgaag gcgaccagca ccttgtaggt gcccgcgggc
     1981 acgcccgtca gcgtgaactg gccggtgacg tccggggtcg ccggcggggg cgggtcgcgc
     2041 aggccgagcg gcaccgggcc gcgctctagc cccgcgttgt agacgctgct gggcaccagc
     2101 acgacgctcg tcatgctgcc gcccggcgcg ttgacgatgc cgatgctgcc ggtgacggtg
     2161 ccgagcgcgg cgacatcgac ggtgacgatc gccaggtcga cgtcgacgac gtcggccgcc
     2221 gcgacggtga ccggcgccgg cgcgagctcg acgccctgac gatatccgcg gacctcgacc
     2281 gcgccgcttt gtacgttgaa cagcgtgaac gcgcccgagg cgtcggcgat cgcgtacggg
     2341 gccggagcag cgccgccctc ggcgacgacg agcgtgccgg ccgcgtcggc gccgacgctg
     2401 cccgagacgc tgacgccctg cgcggccgcg ccgaggggga tcagcgcgac gctggtcgcc
     2461 gcggtgtcga tcacctcgac cgtgtaggtc atcggcgggt cggcgtcgtc ctcggcgtcc
     2521 ttgctcgcga gcgccggctc ggggccgtcg gggttgggca ccgcctcgga gaggtcgatc
     2581 ggcagggccg ggcgcgggcc ggcggggaac ggttgatagt tccgcgcgct ggcgatcagc
     2641 gtccacttga gcgactcggc gaagctgccg tcggggttgc gacgcacgcg gatcggcagg
     2701 acatagtcgc cgcacgagtc ggtcgcggcg acgtcggtca ccggctcgcc gttctcgttg
     2761 agcgcggcga ccagcgcggc ctcgatgccg aggccggtca gcgagtcctc gacgcgcccg
     2821 cggatctgca gggccgcgcc gcacacgtga tcgtcgctgt cgaccacggg gtcgcatacc
     2881 aggcccggcg cgcagatcgg ctgcgggtcc ttgtcgtcct ccgggtcggg cgtgtccgcg
     2941 gcggcgacgc ggtcggggtc acaggccgcg cccggggcga gcgtcggctg
//
LOCUS       DASWNJ010001042         2058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_230445, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001042 DASWNJ010000000
VERSION     DASWNJ010001042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230445"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="VGB85_17590"
     CDS             <1..1032
                     /locus_tag="VGB85_17590"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY0135905.1"
                     /translation="PRNIIHDAHPAEWLTVSEVLAASSNICTTKIADRLGKQTLHRFV
                     EKFHFGERPPIELPGSAPGMLAPPEKWSDIQAANISFGQGMSASPLQVTAAFAAIANE
                     GIYHRPTIVQRVLDASGEPLPRVRPEGERLVRASTARTVLRMLESVVHTKLGTGKNAY
                     VEGYRVAGKTSTAQKSGKQGYDDQQWYSSFLGAVPARAPRVVIMISVDSPEGAHYGND
                     VAAPSFARLAGQIMVHLGVPRDDGTAPPPPKAMTLAASDPKLELGFIPDLDVEPDLPG
                     HRHRPAVTVATGLPDFTGLTLAQALDLAHDAKLELRAVGTGIAVLQDRQPGPVDSGSV
                     VQVWFEPPT"
     gene            1080..1757
                     /locus_tag="VGB85_17595"
     CDS             1080..1757
                     /locus_tag="VGB85_17595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135906.1"
                     /translation="MRPLFRIFTAALLAACNPDDKAMTSETSGTSGGVASDTPTTSGG
                     TTGALTTTSSGDSTGDTTGADATCMCAAFSEIEGGCANDSVWMHVAQCELDTICPPLT
                     VQCPRPGADLYDCQGELVFAEDAMTCALAALRDRTPGRFIVEGTEAGMFYFGTLYGLR
                     LRGDEVAVVTECRSGEGTGWHASDRPLAPPAHFAGCLAMPKPLARHGCLLQGLGDPVV
                     LPECAGE"
     gene            1814..1942
                     /locus_tag="VGB85_17600"
     CDS             1814..1942
                     /locus_tag="VGB85_17600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135907.1"
                     /translation="MRRLGVLVLGCMTCGPTSPVPADSDLVDTWFAGLLVPPRTNF"
BASE COUNT          295 a          816 c          657 g          290 t
ORIGIN      
        1 ccgcgcaaca tcatccacga cgcccacccg gccgagtggt tgacggtcag cgaggtgctc
       61 gccgcctcgt cgaacatctg caccaccaag atcgccgacc gcctcggcaa gcagacgctg
      121 caccgcttcg tcgagaagtt ccacttcggc gagcgcccgc cgatcgagct cccgggctcg
      181 gccccgggca tgctcgcgcc gcccgagaag tggtccgaca tccaggccgc caacatctcc
      241 ttcggtcagg gcatgagcgc ctcgccgctg caggtgaccg ccgccttcgc cgcgatcgcc
      301 aacgagggca tctaccaccg gccgacgatc gtgcagcggg tgctcgacgc cagcggcgag
      361 ccgctgccgc gcgtgcgccc cgagggcgag cggctggtcc gggcctcgac cgcgcgcacc
      421 gtcctgcgca tgctcgagtc ggtcgtgcac accaagctcg gcaccggcaa gaacgcgtat
      481 gtcgagggct accgggtcgc cggcaagacc tcgaccgcgc agaagtccgg caagcagggc
      541 tacgacgacc agcagtggta ctcgagcttc ctcggcgccg tgccggcccg agccccgcgc
      601 gtcgtcatca tgatctccgt cgacagcccc gagggcgcgc actacggcaa cgacgtcgcc
      661 gccccgagct tcgcccgcct cgccggccag atcatggtcc acctcggcgt tccacgcgac
      721 gacggcaccg caccgccgcc gccgaaggcg atgaccctcg ccgcctccga cccgaagctc
      781 gagctcggct tcatccccga cctcgacgtc gagcccgacc tccccggcca ccgccaccgc
      841 cccgccgtca ccgtcgccac cggcttgccc gacttcaccg gcctgaccct cgcgcaggcc
      901 ctcgacctcg cccacgacgc caagctcgag ctgcgcgccg tcggcaccgg catcgccgtg
      961 ttgcaggacc gccagcccgg cccggtcgac agcggctcgg tcgtacaggt gtggttcgag
     1021 ccgccgacct gaccaaaaag ccaggtgact gcgcgcggcc gtcgtgctca aatgcaaacg
     1081 tgcgccccct gttccggatc ttcaccgccg cgctgctcgc cgcctgcaac cccgacgaca
     1141 aggcgatgac gtcggagacc tccggcacct ccggcggcgt cgcgtcggac acgccgacca
     1201 cctccggtgg aacgaccggc gcgctcacca cgaccagctc cggcgacagc accggcgaca
     1261 ccaccggcgc ggacgcgacg tgcatgtgcg ccgccttctc cgagatcgaa ggcggctgcg
     1321 cgaacgacag cgtgtggatg cacgtcgcgc agtgcgagct cgacacgatc tgtcccccgt
     1381 tgacggtgca gtgcccgcga ccgggcgccg acctctacga ctgtcagggc gagctcgtgt
     1441 tcgccgagga cgcgatgacc tgcgcgctcg cggccctgcg cgaccggacc cccgggcgct
     1501 tcatcgtcga gggcaccgag gcgggcatgt tctacttcgg gaccctctac ggcctgcgcc
     1561 tgcgcggcga cgaggtcgcc gtcgtgaccg agtgtcgtag cggcgagggc accggctggc
     1621 acgccagcga tcgcccgctc gcccctcccg cccacttcgc gggctgcctg gcgatgccaa
     1681 agcccctcgc gcgccatggc tgcctcctcc agggcctggg cgaccccgtc gtcttgccgg
     1741 aatgcgcggg cgaatgagac ccgcgctgac acgcgcgcgg ccgcccttca acgctggagg
     1801 gatgcagctc tccatgcgcc ggcttggcgt cctcgtcctc ggatgcatga cctgcggccc
     1861 gacctcgcct gtgcctgcgg acagcgacct cgtcgacacc tggttcgcgg gcctgctcgt
     1921 cccgccgcgc acgaacttct gactcaggtc gtgccgggcg tcttgaccag gcggccgatc
     1981 aagttcggga tcaacggcgc cgcgcgcatc ggggccgtgc ccttgttgtc tttatgttgg
     2041 tcctgcgggg tcggtcgc
//
LOCUS       DASWNJ010001043         1724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_331270, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001043 DASWNJ010000000
VERSION     DASWNJ010001043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331270"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..961)
                     /locus_tag="VGB85_17605"
     CDS             complement(47..961)
                     /locus_tag="VGB85_17605"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEY0135908.1"
                     /translation="MATTYSRKDYPPVPDIRTIGIIGAGQMGGGIAQVAAAAGYEVKL
                     TDVSSQQLDAAMERMDGLLDRQVRKGAIGQEAKRATLARVAAGTDQASLGECELVIEA
                     AAESEELKKRIFQDLVPRIRPDAILASNTSSISITRLAAVTGRPERFIGMHFMNPVPA
                     MRLVELIRGIATEEDTYQAVKGVAERLGKTSVTAEDVPAFIVNRILLPMINEAVYTLY
                     EGVGTVESIDTAMRLGANHPMGPLELADFIGLDTCLSVMQVLYDGLADSKYRPCPLLV
                     KYVEAGWLGRKAKRGFYDYRGEKPVPTR"
     gene            complement(976..>1724)
                     /locus_tag="VGB85_17610"
     CDS             complement(976..>1724)
                     /locus_tag="VGB85_17610"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY0135909.1"
                     /translation="EPTAESLAALLETLAPAYDYLLAASTTFGRNVLPRVAAKLDVAQ
                     LSDIVEVLGPDTFKRYVYAGSALATVKSADAKRVITVRTTAFEAAPAEGGSAEVVTAQ
                     AVAASDLAEFLGREVGGGDRPDLTSARVVVSGGRALGSAENFKIVELLADALGAAVGA
                     SRAAVDAGYAPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKVIVAINKDEEA
                     PIFQVADYGLVGDLFKIVPELADELKRAKA"
BASE COUNT          231 a          588 c          630 g          275 t
ORIGIN      
        1 ctgacggccg ggccaccctc cccctcaaag gggggaggga agcgcgctac cgcgttggga
       61 ccggcttctc gccgcggtaa tcgtagaagc cgcgcttggc cttgcggccg agccagccgg
      121 cctcgacgta cttcaccagc agcggacagg gccggtactt gctgtcggcg aggccgtcgt
      181 agagcacctg catgaccgac aggcaggtgt cgaggccgat gaagtcggcc agctccagcg
      241 ggcccatcgg atggttggcg ccgagcctca tggcggtgtc gatgctctcg accgtgccga
      301 cgccctcgta gagggtgtag accgcctcgt tgatcatcgg cagcaggatg cgattgacga
      361 tgaaggccgg gacgtcctcg gcggtgacgg aggtcttgcc gagccgctcg gccaccccct
      421 tgaccgcttg gtaggtgtcc tcttcggtcg cgatgccgcg gatgagctcg acgagccgca
      481 tcgccggcac cgggttcatg aagtgcatgc cgatgaagcg ctcgggccgg ccggtgaccg
      541 cggccaggcg cgtgatcgag atcgaggagg tgttggaggc gaggatcgcg tccgggcgga
      601 tgcgcggcac gaggtcctgg aagatgcgct tcttgagctc ctcgctctcg gccgcggcct
      661 cgatgacgag ctcgcactcg ccgagcgacg cctggtcggt gccggcggcg acgcgcgcga
      721 gcgtcgcccg cttcgcctcc tggccgatgg cgcccttcct cacctgccgg tcgagcagcc
      781 cgtccatccg ctccatcgcg gcatccagct gctgcgagga gacgtcggtg agcttcacct
      841 cgtagccggc ggccgccgcg acctgggcga tgccgccgcc catctggccg gcgccgatga
      901 tgccgatcgt ccggatgtcc gggaccggcg ggtagtcctt gcgcgagtag gtggtggcca
      961 tcacggctcc gggtctcagg ccttggcccg cttcagctcg tcggcgagct cgggcacgat
     1021 cttgaacagg tcgccgacca ggccgtagtc ggcgacctgg aagatcggcg cctcctcgtc
     1081 cttgttgatc gcgacgatga ccttgctgtc cttcatcccg gccaggtgct ggatggcgcc
     1141 ggagatgccg accgctatgt agagttccgg cgccacgatc ttgccggtct ggccgacctg
     1201 catgtcgttg ggcgcgtagc cggcgtcgac cgcggcgcgg ctggcgccca cggccgcacc
     1261 gagcgcgtcg gccagcagct cgacgatctt gaagttctcg gccgagccca gcgcccgccc
     1321 gcccgagacg acgacccggg cggaggtcaa atccggccgg tcgccgccgc cgacctcgcg
     1381 gccgaggaac tcggccaggt cggacgcggc caccgcctgg gcggtgacca cctcggccga
     1441 gccgccctcg gccggtgccg cctcgaaggc ggtggtgcgg acggtgatga cgcgcttggc
     1501 gtcggcgctc ttgacggtgg ccagcgcgct gccggcgtag acgtagcgct tgaaggtgtc
     1561 gggccccagc acctcgacga tgtcgctcag ctgcgccacg tcgagcttgg ccgcgacccg
     1621 cggcagcacg ttgcggccga aggtggtcga ggcggcgagg aggtagtcgt aggccggagc
     1681 gagcgtttcg agcagcgccg cgaggctctc ggccgtgggt tcgg
//
LOCUS       DASWNJ010001044         2180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_584224, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001044 DASWNJ010000000
VERSION     DASWNJ010001044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584224"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VGB85_17615"
     CDS             <1..822
                     /locus_tag="VGB85_17615"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HEY0135910.1"
                     /translation="VRAAGSDVAASSLLLPAGVALGPGELALLAGCGHSVVYVRRAPR
                     VAILCTGDELVPVGAWPQRGQVISANGLMLAWQAREAGAEVIDLGIARDDPDDLRAAL
                     ERGISGEADVLVTCGGISVGDHDLVLPTLIGLGASLVFRRVRLRPGRPTTMLEAPHSD
                     RPAVPVFALPGNPASAHVAFELFVRPVLRRLSGHLRWQRPQREVVLTEACPRDPRRAH
                     VIRAVVAGDEATPLPDQTSGALRSIAGHNALIELASGTGPAEAGTRVTALLVREA"
     gene            819..1364
                     /locus_tag="VGB85_17620"
     CDS             819..1364
                     /locus_tag="VGB85_17620"
                     /inference="COORDINATES: protein motif:HMM:NF039391.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HEY0135911.1"
                     /translation="MRRIADTAVPGDRSRPADRAPGRLRRLALALALTACRPAGTTSP
                     AGACTGAVTDAAWLAGSWRGQHGEVEEHWTSASGRTMLGLGRTVVGGGTMAFEFMRIE
                     ERADGLVFVAHPSAGPGVEFRRTACRADLLRFENPGHDFPKVIEYRRTGDDTIVANVE
                     GDGGDGGKLGQALELRRERPR"
     gene            1361..>2180
                     /locus_tag="VGB85_17625"
     CDS             1361..>2180
                     /locus_tag="VGB85_17625"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY0135912.1"
                     /translation="MSGDARDIMLVVVTEDAGERADVVLGRRVPGLSRRVARSLALAG
                     RLKIDGVSAAPAHRVRAGEQLILRVPVAAVETPPLEILAVTQRLVYVDKPSGLHTHRL
                     RPTDPPALADLVAAAFPECAAASEDPREGGAVHRLDRGTSGVVAFARSPEVHAAARAA
                     FAAGVVRKRYLALVTCPEDQAWPPPVHRWVVPDGDTIEVRAPLGPGDQPSRVAVRPDG
                     QPSLSVVEPPRPRGPGQASVALALHTGRRHQARVHLAWLGLPIVGDDLYGGSPAD"
BASE COUNT          235 a          873 c          791 g          281 t
ORIGIN      
        1 gtgcgcgccg cggggtccga cgtcgccgcg agctcgctgc tgctgccggc cggcgtcgcg
       61 ctcgggcccg gcgagctcgc gctgctcgcc ggctgcgggc actcggtcgt gtatgtgcgt
      121 cgcgccccac gcgtggcgat cctgtgcacc ggcgacgagc tggtcccggt cggcgcgtgg
      181 ccgcagcgcg gccaggtgat cagcgccaac gggctgatgc tcgcctggca agcgcgcgag
      241 gccggcgcgg aggtcatcga cctcggcatc gcccgcgacg atcccgacga cctgcgcgcc
      301 gcgctcgagc gtgggatctc cggcgaggct gacgtgctcg tcacctgcgg tgggatctcc
      361 gtcggggatc acgacctcgt gctgccgacc ctgatcggcc tcggcgcgtc gctggtgttt
      421 cgccgcgtgc gcctgcgccc cggacgaccg acgacgatgc tcgaggcccc gcacagcgac
      481 cgccctgccg tccccgtgtt cgccctgccc ggcaaccctg cgagcgccca cgtcgccttc
      541 gagctgttcg tgcgcccggt gctgcggcgc ctgtcgggcc acctgcgctg gcagcggccg
      601 cagcgtgaag tggtgctgac cgaggcgtgc ccgcgcgacc cccggcgcgc ccacgtgatc
      661 cgggccgtgg tcgccggcga cgaggcgacc cccctgcccg accagacctc gggcgcgctg
      721 cggtcgatcg ccggccacaa cgccctgatc gagctggcct cggggacagg tccggccgag
      781 gccggaacgc gggtcaccgc gttgctggtg cgagaggcat gagacggatc gccgatacag
      841 ctgtccccgg ggacaggtcc cgcccggccg accgcgctcc cggccgcctg cggcgcctcg
      901 ccctcgcgct cgcgttgacc gcatgtcgtc ccgcgggcac cacgagcccg gcgggcgcct
      961 gcaccggcgc ggtgaccgac gccgcctggc tggctggctc gtggcgcggc cagcatggcg
     1021 aggtcgagga gcactggacc agcgcgagcg gtcgcacgat gctcgggctc ggccgcaccg
     1081 tcgtcggtgg cggcacgatg gccttcgagt tcatgcgcat cgaggagcgc gccgacggcc
     1141 tggtgttcgt cgcccacccc tccgccggcc ccggggtcga gtttcgccgc accgcctgcc
     1201 gcgctgacct gctgcgcttc gagaaccccg ggcacgactt cccgaaggtg atcgagtacc
     1261 gccgcaccgg cgacgacacg atcgtcgcca acgtcgaggg tgacggcggc gacggcggca
     1321 agctcggcca ggcgctcgag ctgcgccgcg agaggccgcg gtgagcggcg acgctcgcga
     1381 catcatgctc gtcgtcgtca ccgaggacgc gggcgagcgc gccgacgtag tgctcggccg
     1441 ccgcgtgccc ggcctgtcgc gccgggtcgc ccgcagcctc gcgctcgccg gacgcctgaa
     1501 gatcgacggc gtatctgctg cgcctgccca ccgcgtccgc gcgggcgagc agttgatcct
     1561 ccgggtgccc gtcgccgcgg tcgagacccc gccgctggag atcctcgcgg tcacgcagcg
     1621 cctggtgtac gtcgacaagc cctccggcct gcacacccac cgcctgcgcc ccaccgaccc
     1681 gcccgcgctc gccgacctcg tcgccgccgc gttccccgag tgcgcggccg ccagcgagga
     1741 cccgcgcgag ggcggcgcgg tgcaccgcct cgaccgcggc acctcgggcg tcgtcgcctt
     1801 cgcgcgcagc cccgaggtcc acgcggccgc ccgcgccgcg ttcgcggccg gcgtggtgcg
     1861 caagcgttac ctggccctcg tgacatgtcc cgaggaccag gcgtggccgc cgcccgtcca
     1921 tcgctgggtc gtgccggacg gcgacacgat cgaggtccgc gccccgctcg gccccggcga
     1981 ccagcccagc cgcgtcgccg tgcgccccga cggccagccg agcctcagcg tcgtcgagcc
     2041 cccgcgcccg cgcggccccg ggcaggcgag cgtcgccctg gcgttgcaca cgggccgccg
     2101 tcaccaggcc cgcgtgcacc tggcgtggct cggcctgccg atcgtcggcg acgatctgta
     2161 tggcggcagc ccggccgatc
//
LOCUS       DASWNJ010001045         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_196341, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001045 DASWNJ010000000
VERSION     DASWNJ010001045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196341"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..>2206
                     /locus_tag="VGB85_17630"
     CDS             61..>2206
                     /locus_tag="VGB85_17630"
                     /inference="COORDINATES: protein motif:HMM:NF019298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEY0135913.1"
                     /translation="MSYDPWFAESGFGHGSGSGSGYGYGGRSTRVPSIRAAGQQPARS
                     RFETTPVFLADLAVDSSGEATVKAKLPDNLSTFRITAIASARLVDGTSPGRFGKNDAR
                     TVVTAPLVLRAAMPRALRPGDRAELAAIVQNNTGVAGRLSVTARVVEEPGSKGHVLKL
                     LSPATASAEVTPGDQTRLTFQIEATAPGSPDFELQAALTPTAGGAKITDGLRLPLPVQ
                     PERTLTERVAAYGAVTNDQAVAIPIKLPTDVLRGHGGVTVSTTSSLLGGLEDAVHALV
                     QYPYGCVEQTASRLLPIVALGALEDYPLGVDDPKDFLKIGVERILAMQTDDGGFGYWP
                     GSKEAHVYASAYATWILDLAKKAGHPVPQAALDRALKFLETRVNGLVLTQLPVDWGYH
                     DGVRVAIALHVLADAGRDIHEQAAGLYTLRQQLPLFARAFLLMAIHRFDPHSLEVRTL
                     QSELLGNIAELQATAHTSEAGRYGLDEFFASDGRSDAITLMALLRVQPDHPVVVKLAR
                     GLLERRIGGAWRNTQENAYALVALADYARVYEAELPDFTARAWVGRTNVLDAQFKGRE
                     LATRTSFTDMATILDLPREPGAALLPVVLQRQGQGRLYYRLAAEWAPSQVDLPARNQG
                     FVVTRALRTSAGPATTSVPAGQPIAMDIKISSKIRVRYVVLDVPLPAGLEGVSRTLGV
                     GRSAAVLGGRRGWWVSHEEQRADRVVVFADDLP"
BASE COUNT          324 a          916 c          675 g          291 t
ORIGIN      
        1 cgcaccgccg agacccgcca gctgcacgac taccgccatt tgctttaccc ctacgtcccc
       61 atgtcatacg acccctggtt cgccgagagc ggcttcggtc acggctcggg cagcggctcg
      121 ggctacggct acggcggtcg aagcacccgg gtgcccagca tacgcgccgc cgggcagcag
      181 cccgcgcgca gccgcttcga gaccaccccc gtgttcctcg ccgacctcgc cgtcgacagc
      241 tccggcgagg cgaccgtcaa ggcgaagctg ccggataatc ttagcacctt tcgcatcacc
      301 gccatcgcct cggcgcggct cgtcgacggc acttcaccgg gccgcttcgg caagaacgac
      361 gcgcgcaccg tcgtcaccgc gccgctggtc ctgcgcgccg cgatgccgcg agccctgcgc
      421 cccggcgacc gcgccgagct cgcggcgatc gtgcagaaca acaccggcgt cgccggccgc
      481 ctcagcgtca ccgcgagagt ggtcgaagag ccaggttcga agggacatgt cctcaagctc
      541 ctgtcccccg cgacagccag cgccgaggtc acccccggcg accagacccg cctcaccttc
      601 cagatcgaag cgaccgcccc cggcagcccc gacttcgagc tgcaagccgc gctgaccccc
      661 accgccggcg gcgcgaagat cacggacggc ctgcgcctgc cgttgccggt ccagcccgag
      721 cgcaccctca ccgagcgcgt cgccgcctac ggcgccgtca ccaacgacca ggccgtggcg
      781 atcccgatca agctcccgac cgacgtgctg cgcggccacg gcggcgtcac cgtatcgacc
      841 acctcgagcc tgctcggcgg cctcgaggac gccgtgcatg cgctcgtgca atacccctac
      901 ggctgcgtcg agcagaccgc gagccgcctg ctcccgatcg tcgccctcgg cgcgctcgag
      961 gactacccgc tcggcgtcga cgacccgaaa gacttcttaa agatcggcgt cgagcggatc
     1021 ctcgccatgc agaccgacga cggcggcttc ggctactggc ccggcagcaa ggaggcccac
     1081 gtctacgcct ccgcctacgc gacgtggatc ctcgacctcg ccaaaaaggc cggccacccg
     1141 gtcccgcagg ccgccctcga ccgcgcgctg aagttcctcg agacccgcgt gaacggcctc
     1201 gtgctcaccc agctcccggt cgactggggc taccacgacg gcgtgcgcgt ggcgatcgcg
     1261 ctgcacgtgc tcgccgacgc cggccgcgac atacacgagc aggccgccgg cctctacacc
     1321 ctgcgccagc agctgccgct gttcgcccgc gccttcctgc tgatggcgat ccaccgcttc
     1381 gacccgcaca gcctcgaggt ccgcacgctg cagtcggagc tgctcggcaa catcgcggag
     1441 ctgcaggcca ccgcgcacac cagcgaggcc ggccgctacg gcctcgacga attcttcgcc
     1501 tccgacggcc gcagcgacgc gatcaccctc atggccctgc tgcgcgtcca gcccgaccac
     1561 ccggtcgtcg tcaagctcgc gcgcggcctg ctcgagcgcc ggatcggcgg cgcgtggcgt
     1621 aacacccagg agaacgccta cgcgctggtc gccctcgccg actacgcccg cgtgtacgag
     1681 gccgagctgc ccgacttcac cgcgcgcgcc tgggtcggcc gcaccaacgt cctcgacgcg
     1741 cagttcaagg gccgcgagct cgccacccgc accagcttca ccgacatggc gacgatcctc
     1801 gacctgcccc gagagccagg tgccgcgctg ctcccggtcg tcttgcagcg ccagggccag
     1861 ggccgcctct actaccgcct cgccgccgaa tgggcgccct cccaggtcga cctgcccgcg
     1921 cgcaaccagg gcttcgtcgt cacccgcgct ctgcgtacca gcgccggccc ggcgaccacc
     1981 tcggtccccg ccggccagcc gatcgccatg gacatcaaga tctcgagcaa gatccgcgtg
     2041 cgctacgtcg tcctcgacgt gccgctcccg gccggcctcg agggcgtctc gcgcaccctc
     2101 ggcgtcggcc gcagcgccgc ggtcctcggt ggccgccgcg gctggtgggt ctcgcatgag
     2161 gagcaacgcg ccgaccgcgt cgtcgtgttc gccgacgacc tcccac
//
LOCUS       DASWNJ010001046         5644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_302430, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001046 DASWNJ010000000
VERSION     DASWNJ010001046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302430"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VGB85_17635"
     CDS             complement(<1..494)
                     /locus_tag="VGB85_17635"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0135914.1"
                     /translation="MVRPLATRSASFRLYLARHEDDPESGAPSYVVKLLVPGRSPDHA
                     HLAAQFEHEIKLLRAFNHPGIPSVHGDGEKDGVRYLVMDYIDGCDLAALLGHDMGLSG
                     GARSVSREIAVYVMAQLADALNHIHTFEVLDETTHKFGELGIVHRDVCPSNVLLSRTG
                     NVLLA"
     gene            592..1914
                     /locus_tag="VGB85_17640"
     CDS             592..1914
                     /locus_tag="VGB85_17640"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0135915.1"
                     /translation="MASPSPPVVVLGAGLTGLSAAYHLRRPAVLVERTSEPGGHARST
                     RQAGFTFDVTGHWLHLRDPQTRGLVAGLFAPDELVSVERRTRVFTHGVLLPYPFQANL
                     YGLPLAVVQECLVEFVAARERAARGEQPAPTFEAFAEARFGRGIARHFFVPYNTKLWG
                     MHPNRLTAEWVSRYIPVPDVTQVIGGAIGVPQEGLGYNASFLYPRAGGIDALPRRLAA
                     GLPAGTEVLLGTDVEVVDPANHRVKLTSEPDWREYHALISTIPLPELVRRIVGAPAEV
                     REAAAALRWVRWRWLDVATRTPVPADYHWVYVPETRYPFFRVGVYSNAVASMAPEGCS
                     SLYVELAERDAEIDVPEVLRALVEIGALASVDDVLFTAERRAEYAYVVFDDAYAAATS
                     TILGWLERVGIRSCGRYGAWVYNAMEDCMIAGREAAAWAEGHVRGDRP"
     gene            1911..2654
                     /locus_tag="VGB85_17645"
     CDS             1911..2654
                     /locus_tag="VGB85_17645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0135916.1"
                     /translation="MIELSVVIPVYNEEAILEASVTELIRGFADMDLSAYEIVLAENG
                     SRDRTRELAARLASEHPQVRTFSCAAPNYGAALREGIEGARGEYVLCEEIDLCDLEFH
                     RRALALLRAGTVDLVIGSKAMRGAHDRRPLARRAATRIYNRMLRVTLGFSGTDTHGLK
                     AFRRAALAPVVARCIVGHDVFASELVIRAEREGLRVREIPVELEEKRPPSVHLLRRVP
                     RVLHHLLRLMAAIHLGVGADRSRTSAREP"
     gene            2662..3549
                     /locus_tag="VGB85_17650"
     CDS             2662..3549
                     /locus_tag="VGB85_17650"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY0135917.1"
                     /translation="MSDQSTIVSVDLDDLACYHAIHGLPPPGPSAAGVVLERSLPRFL
                     ELFSELGVRATFFVIGRDLQRDAESGGKGSALLRQALAAGHELGNHSFSHAYDMVTWT
                     PAAIYEDLRRCDVLLRGLGARPAGFRAPGYTHDALLLQQVAALGYRYDSSLLPSPAYY
                     AAKLAAIGWMAVRGRRSQSLAHGWRSFVGPTRPHFMADCGLWEVPVSVSSLLRLPMVG
                     TFVLGGRGVLLREATTTRYLHLELHGLDLVDANEVTDAALVSKAPELKTPLETRLARL
                     RGLLKARQGATSIGGAFAS"
     gene            complement(3500..4219)
                     /locus_tag="VGB85_17655"
     CDS             complement(3500..4219)
                     /locus_tag="VGB85_17655"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HEY0135918.1"
                     /translation="MAWKQFQFKDQTVFVRVLATGKPIVHRGRVEMRYRLGATKSYRA
                     APDNLIDMEGEILPDEDFGGAPVPAVSSQAVREDPVKPAGPATIVIHTDGACSGNPGP
                     AGIGVFIERPDADGIAITEHSEFIGEGTNNIAELTAILRALEMLKPEEQGAHVLLYTD
                     SAWSLGVLVGGWKAKANLELINAIQERMKSLPDVELLKVRGHAGNSGNEEADALATMA
                     VRRESSTTRTRRRSKSPPAAP"
     gene            4388..5491
                     /locus_tag="VGB85_17660"
     CDS             4388..5491
                     /locus_tag="VGB85_17660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135919.1"
                     /translation="MKQKILVSLAATALLGLTACKGGATAAGGKLIPEQATIMVGIDV
                     GGLMKTKLYGENKAEFEKQKDYSEMAAAAKACNLDPEKALNSVLIGTDGKANVVAVLT
                     GEGLGDEKNLTCIADKAKEKNGGKTPFTIVDEAGKKTLKLESGEGTGYIVDGKTVVFA
                     SKAWEGAVKDLIDGKGKSANDGPNKDLFDRADRSKHIWAAGLVPAEMAGGAKSMGADA
                     KDFSVSFDLSDGLALKAMVGLASADQAKDLKKKGDDNLPGAKMMAGMMGVPPKALETV
                     KIDTKDNMITVEASLSNDDLKIIKDKAGAMMGGAGGGGDMGGMPMDKPPLPAEAAPVG
                     DAGAAGAPAGTPPAGGDAAGGTPPAAPAPTPAQ"
BASE COUNT          822 a         1889 c         2045 g          888 t
ORIGIN      
        1 gccaggagaa cgttgccggt gcgcgacagg agcacgttgc tcgggcagac gtcgcggtgg
       61 acgatgccga gctcgccgaa cttgtgggtg gtctcgtcga ggacctcgaa ggtgtggatg
      121 tggttgaggg cgtcggcgag ctgggccatc acatagacgg cgatctcgcg ggacaccgag
      181 cgggctccgc cgctgaggcc catgtcgtgg ccgagcagcg cggcgaggtc gcagccgtcg
      241 atgtagtcca tcacgaggta gcggacgccg tccttttcgc cgtccccgtg gaccgagggg
      301 atccccgggt ggttgaaggc ccgcaggagc ttgatctcgt gctcgaactg cgccgcgagg
      361 tgcgcgtggt cggggctgcg gccggggacc agcagcttga ccacatacga gggggcgccg
      421 ctctcggggt cgtcctcgtg gcgcgccagg tacaggcgga agctcgcgct gcgggtggcc
      481 aggggccgga ccaagcgaaa cgaaccgaga ctctgcagct ccatgggcac cgccagtgtg
      541 catgccggag gatccgctgt ccatcgccct gtctggtaaa accggacgcc catggcatcg
      601 ccctcgcccc cggtcgtggt cctcggcgcc ggcctgaccg gcctgtccgc ggcctaccac
      661 ctgcgccgcc cggccgtact ggtcgagcgc acctcggagc cgggtggcca cgcccgcagc
      721 acccgccagg ctggcttcac cttcgacgtg accggccact ggctgcacct gcgcgacccg
      781 cagacccggg gcctggtggc agggttgttc gcccccgacg agctggtcag cgtcgagcgg
      841 cgcacgcggg tcttcacgca cggggtgctg ctgccgtacc ccttccaggc caatttgtac
      901 ggcctgccgc tcgcggtcgt gcaggagtgt ctggtggaat tcgtggcggc gcgcgagcgg
      961 gcggcccgtg gtgagcagcc tgcgccgacc ttcgaggcct tcgccgaggc gcgcttcggt
     1021 cgggggatcg cgcggcactt ttttgtgccg tacaacacca agctgtgggg catgcacccg
     1081 aaccggctca ccgccgagtg ggtctcgcgc tacatcccgg tgcccgacgt cacccaggtg
     1141 atcggtgggg cgatcggtgt gccgcaggag gggctcggct acaacgcgag ctttttgtat
     1201 ccgcgagccg gcggcatcga cgcgttgccg cggcggctcg cggcagggtt gcctgcgggg
     1261 accgaggtct tgctcggtac ggacgtcgag gtggtcgacc ccgccaacca ccgcgtcaag
     1321 ctgaccagcg agcccgactg gcgcgagtat cacgcgctga tctcgacgat cccgctgccc
     1381 gagctggtcc gtcggatcgt cggcgcgccg gccgaggtgc gtgaggccgc ggcggcgctg
     1441 cgctgggttc gctggcgctg gctcgacgtc gccacccgca ctcctgtgcc cgccgactat
     1501 cactgggtct acgtgcccga gacgcgctac ccgttctttc gcgtcggcgt gtattcgaac
     1561 gcggtcgcca gcatggcgcc ggaggggtgc agcagcctct acgtcgagct cgccgagcgc
     1621 gacgcggaga tcgacgtgcc ggaggtgttg cgcgcgctcg tcgagatcgg cgcgctcgcc
     1681 tcggtcgacg acgtgctgtt caccgcggag cgccgggccg agtacgccta cgtcgtgttc
     1741 gacgacgcct acgcggcggc gacgagcacg atcctcggct ggctcgagcg agtggggatc
     1801 cgcagctgcg ggcgctatgg tgcgtgggtc tacaacgcga tggaggactg catgatcgcg
     1861 gggcgcgagg ctgcggcctg ggccgaggga catgttcgag gggacaggcc atgatcgagc
     1921 tcagcgtcgt cattccggtg tacaacgagg aggcgatcct cgaggcgtcg gtcacggagc
     1981 tgatccgcgg cttcgccgat atggacttga gcgcctacga gatcgtgctc gccgagaacg
     2041 gcagccgcga ccgcacccgc gagctcgcgg cgcggctggc gagcgagcac ccgcaggtgc
     2101 gcaccttctc gtgcgccgcg cccaactacg gggccgcgct gcgcgagggc atcgaggggg
     2161 cgcgcggcga gtatgtgctg tgcgaggaga tcgatctgtg cgacctcgag tttcaccggc
     2221 gggcgctcgc gctgctgcgg gccgggacgg tcgacctcgt gatcggctcc aaggcgatgc
     2281 gcggcgcgca cgaccggcgc ccgctcgccc gccgcgcggc gacccggatc tacaaccgca
     2341 tgctgcgcgt cacgctgggc tttagcggca cggacaccca cgggctcaag gcgtttcgcc
     2401 gggccgcgct ggcgccggtg gtcgcgcgct gcatcgtcgg gcacgacgtc ttcgcctcgg
     2461 agctggtgat ccgggccgag cgcgagggcc tgcgggtgcg ggagatcccc gtcgagctcg
     2521 aggagaagcg gccgccctcg gtgcacttgc tgcgccgcgt cccgcgggtg ctacatcatc
     2581 tgctccgcct gatggcggcg atccacctcg gcgtcggggc ggatcgatct cgaacatccg
     2641 cgcgagagcc gtgagcacac gatgagcgac cagagcacga tcgtcagcgt cgacctcgac
     2701 gacctcgcct gttaccacgc gatccacggg ctgccgccgc cgggtccgtc ggccgcgggg
     2761 gtggtgctcg agcggagcct tccacgtttt ctcgagctgt tttcggagct cggcgtgcgc
     2821 gcgaccttct ttgtcatcgg tcgcgacctc cagcgcgacg ccgagtctgg cggcaagggc
     2881 agcgcgctgc tgcggcaggc gctcgcggcc gggcacgagc tcggcaacca cagtttttcg
     2941 cacgcctacg acatggtcac ctggacgccg gcggcgatct acgaggacct gcgccgctgc
     3001 gacgtgctgt tgcgcgggct cggggcgcgg ccggcgggct ttcgggcgcc gggctacacg
     3061 cacgacgcct tgctgctgca gcaggtcgcg gcgctcgggt atcgctacga ctcctcgctg
     3121 ttgccctcac cggcgtacta cgcggccaag ctggctgcga tcggttggat ggcggtccgc
     3181 gggcggcgct cgcagtcgct ggcgcatggc tggcgcagct tcgtcgggcc gacgcggccg
     3241 cacttcatgg cggactgcgg gctgtgggag gtgccggtca gcgtgtcgtc gctgctgcgc
     3301 ctgccgatgg tcgggacctt cgtgctcggc gggcgcggcg tgctgctgcg cgaggcgacg
     3361 acgacgcggt atctgcatct cgagctgcac ggcctcgacc tcgtcgatgc gaacgaggtg
     3421 accgacgccg cgctggtgag caaggcgccg gagctgaaga cgccgctgga gacgcggctg
     3481 gcgcggctac gcgggctgct caaggcgcgg cagggggcga cttcgatcgg cggcgcgttc
     3541 gcgtcgtaga gctctcgcgg cggacggcca tcgtggcgag ggcgtcggcc tcctcgttgc
     3601 cgctgttgcc ggcgtggccg cggaccttga gcagctcgac gtccggcagg gacttcatgc
     3661 gctcctggat cgcgttgatc agctcgaggt tggccttggc cttccagccg ccgacgagca
     3721 cgccgaggct ccacgcgctg tcggtgtaga gcagcacgtg ggccccctgc tcctcgggct
     3781 tcaacatctc gagcgcgcgc aggatcgcgg tcagctcggc gatgttgttg gtgccctcgc
     3841 cgatgaactc cgagtgctcg gtgatcgcga tgccgtcggc gtcggggcgc tcgatgaaca
     3901 cgccgatgcc ggcaggcccg ggattacccg agcacgcgcc atcggtgtgg atgacgatcg
     3961 tggccggtcc cgccggtttg acagggtcct cacgcacggc ctggctcgat accgccggga
     4021 ccggggcgcc gccgaagtcc tcgtcgggga ggatctcgcc ctccatgtcg atgaggttgt
     4081 ccggggcggc gcgatacgac ttcgtcgcgc cgaggcggta tcgcatctcg acgcggccgc
     4141 ggtgcacgat cggcttgccg gtcgccagca cgcggacgaa cactgtttgg tccttgaact
     4201 ggaactgctt ccacgccatg gcagagtctc cgacgagagc gcgcgtcggc cccgaggctg
     4261 ctgcgcggag tatgggatta ccacggaacg gcccccgcgg accggcgaaa aggcacatct
     4321 ttggcgcgta gcgaccccgg ttatttttct tgcgggtcgt aattgagggt cgtataagag
     4381 ccccgctatg aagcagaaga tcctcgtgtc cctcgccgca acggcgctcc ttggtctcac
     4441 cgcgtgcaag ggtggcgcga cagcggccgg tggcaagctc atccccgagc aggcgaccat
     4501 catggtcggc atcgacgtcg gcggtctgat gaagaccaag ctgtacggcg agaacaaggc
     4561 cgagttcgag aagcagaagg actacagcga gatggccgcg gccgcgaagg cctgcaacct
     4621 cgaccccgag aaggccctca actctgtcct gatcggcacc gacggcaagg ccaacgtggt
     4681 cgccgtgctc actggcgagg gtctcggcga cgagaagaac ctcacctgca tcgcggacaa
     4741 ggccaaggag aagaacggtg gcaagacccc gttcaccatc gtcgacgagg ctggcaagaa
     4801 gaccctcaag ctcgagtcgg gcgagggcac cggctacatc gtcgacggca agacggtcgt
     4861 gttcgcgagc aaggcctggg aaggcgccgt gaaggacctg atcgacggca agggcaagtc
     4921 ggcgaacgac ggcccgaaca aggacctgtt cgaccgcgcc gaccgcagca agcacatctg
     4981 ggctgccggt ctcgtgccgg ccgagatggc cggtggtgcg aagagcatgg gcgccgacgc
     5041 caaggacttc tcggtctcgt tcgacctcag cgacggcctc gcgctcaagg cgatggtcgg
     5101 tctcgcctcg gcggaccagg ccaaggacct caagaagaag ggcgacgaca acctgccggg
     5161 cgccaagatg atggccggca tgatgggcgt gccccccaag gcgctcgaga ccgtcaagat
     5221 cgacaccaag gacaacatga tcacggtcga ggcgtcgctc agcaacgacg acctcaagat
     5281 catcaaggac aaggccggcg cgatgatggg cggcgccggt ggcggcggcg acatgggcgg
     5341 catgcccatg gacaagcccc cgctccccgc ggaggccgct cccgtcggtg acgctggtgc
     5401 tgctggtgcg cccgctggca ccccgcccgc cggtggtgac gctgccggtg gcaccccgcc
     5461 ggccgccccc gccccgacgc ccgcccagtg agcgggtcgt gtgagccggc gagtcccccg
     5521 ggacttgcga ggctcctgcg gatcccgtaa acacctgcgg cccggttcaa tcctgaaccg
     5581 ggccgttttg ctttttaaaa gcgcctgcgc gcgcgcgcca ggcccctttc cttgtagctg
     5641 ctcc
//
LOCUS       DASWNJ010001047        10129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_54852, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001047 DASWNJ010000000
VERSION     DASWNJ010001047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 10129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10129
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54852"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VGB85_17665"
     CDS             <1..584
                     /locus_tag="VGB85_17665"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEY0135920.1"
                     /translation="LFDERPETYKTRSAGLGIDLQPYLDAGRLLLRQLDPGEIAPGEF
                     AQQVRAAVEEGQAKVVVLDSINGYFNAMGTSDILVPQLHELLTFLSRSGVLTLVAGSQ
                     EGFMSIGSQGGVDISYLSDTIAVLGYFEHRGEIRRCITAVKKRHGKHGTMIHELTFDA
                     GGVHVGEVLRKYPNLLVGSAGPSVRREDGIDGD"
     gene            601..2214
                     /locus_tag="VGB85_17670"
     CDS             601..2214
                     /locus_tag="VGB85_17670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0135921.1"
                     /translation="MLAPLGRDAAVVGQLLAGAGIVSTVVRDASALITRLANDVGAVL
                     VTEEALTHDGATGLATALQAQPSWSDVPVLLFATGDRQDTAWQSEILRAAGNITVLER
                     PIAGLTLVTAVQSALRARRRQYELRDVLASEQRARLAAEAATRIKDEFLANVSHELRT
                     PLSAILLWGNLLGSGRAGQDDALSAIERSAQAQSKLIEDLLDVSRMLAGKLRVRLRQG
                     QLAPVARAAVEVVQPMAEAKGVRLAAHIDPGAGTVLIDPDRMQQVLWNLLSNALKFTP
                     TGGEVMIRLSRLGGQVRVRVSDTGIGIGAEFMPHLFERFGQADTTSTRSKGGLGLGLA
                     IARQLVELHGGTLRADSRGEGQGAHFTVDLPVLRADAEVAGPDATSGPLRVLPGLRVL
                     LVEDEPDTRRALALVLESAGAEVTAVDSAAAARAVLASLTAARRPGVLVSDIGLPGED
                     GIMLIRAIRAEEARLGETPFPALAVSAYSSEAAREQALAAGFDRYLVKPILPDALVVA
                     ILALAPPQPLLPPLSPLSPLPPLPPLSDA"
     gene            complement(2250..2984)
                     /locus_tag="VGB85_17675"
     CDS             complement(2250..2984)
                     /locus_tag="VGB85_17675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0135922.1"
                     /translation="MARRPIEKHNVDMIPAPNVPSGPVEAGSDEVRRLPLDDVPSVLI
                     AEDHTLVREGLRLLLSPECRVLDAVSDGRLLLEAAERLQPDIILIDIAMPGLNGLEAA
                     RQLGGLCPESRLVFVTSRVERSYVREAFAAGAVGYVTKSSASRELLSAIRVVIAGRRY
                     LSPGLSLTLEDVLDEAPAPDPLTTRQREVLQLVAEGRTARQIAEALHISRKTAEFHKS
                     RLMQVLRLHSTAELTRYALEHGVAGS"
     gene            complement(3168..3572)
                     /locus_tag="VGB85_17680"
     CDS             complement(3168..3572)
                     /locus_tag="VGB85_17680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135923.1"
                     /translation="MKKYMSAVNQGYHALRQFDALSLEEFAQCIDRAIVRLTTAVRIE
                     GVNASWAATEGLVRRWLSHLDTNARTEVFALALSSGWERHSTALARSITKAFTPHLPV
                     SASDCCVSIDHRQVVNAASEGEGDGRLSVLAA"
     gene            3853..5295
                     /locus_tag="VGB85_17685"
     CDS             3853..5295
                     /locus_tag="VGB85_17685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0135924.1"
                     /translation="MFLDSVQDYAFISFDLDRRVVGWSRGAERILGYQEADVLGLPGS
                     VFFTPEDVVREEDEKEFALAREVGRAEDERWHLRRDGSRFWGSGVMTPLVDVAGRLRG
                     YSKVMRDNTARRLADARLRDSEERLRLFSENVGDYALVPVDLEGMVVGWNPGAARIFG
                     YTADEILGQHCARFFTPADVACGEPDRDFRRAIAEGKSEYEREMVRKDGSVFWAQWVT
                     TPIRDEKGQLRGFAKVLQDTTDRKLAQERLRSQVQSTGEALDRTKEELRALAGSLLSA
                     QEDERRRIARELHDDLSQRLAGLANNLAALQDALPDEPARLRTAVQGVEHAVGLLASE
                     VRQLSHRLHPSILDDLGLGPAIRRLVEDFQVGRAEPATFIKHTLPEQIPRDVATAVYR
                     ITQEALRNITKHGNNGLVRVELGGEASALRLVVSDTGPGFDPAMVRGRGGLGIISMQE
                     RAGLVGGNLRLISRRDHGTTIEVRVPLAPT"
     gene            complement(5352..6008)
                     /locus_tag="VGB85_17690"
     CDS             complement(5352..6008)
                     /locus_tag="VGB85_17690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0135925.1"
                     /translation="MPISETAPPASVVHDLERDGVADVFHADEDRGWLFSGLDGAALA
                     AARVDAPASPCSQMSGAEKVLLPVAATRNPVKDDAMSINSRPRLLIAEDHAQVRDGLR
                     ILLNDEFDIIDAVADGQALLEAAARAPPDAVVLDISMPRLNGIEAATRLVRLYPGVGV
                     VFITNHTEPEYLQAALEAGAHGYVIKTSLFRHLIAAVHAALEGQQFVSPLVSLPSARG
                     "
     gene            6104..6847
                     /locus_tag="VGB85_17695"
     CDS             6104..6847
                     /locus_tag="VGB85_17695"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0135926.1"
                     /translation="MITVQHLAKRFTLSRQQQRTLGDHRKPGLVGKKQLQAVDDVGFH
                     CKPGRIFALLGPNGAGKIEIAGVDALADPVAARRKLGFLTGSTKLYERLTAEETLRYY
                     AALAGVAEADYRRRRDALFAALGIDAFADRRVGKLSTGMQQKVSIARTIIHDPEVLVF
                     DEATSGLDVIAARAIIQFIRQCRADGKTVLFSTHIMSEVTMLADDLAIVHGGKLIYSG
                     TFADFTAASGQSSVEEEFVRRIEASEASV"
     gene            6844..8055
                     /locus_tag="VGB85_17700"
     CDS             6844..8055
                     /locus_tag="VGB85_17700"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0135927.1"
                     /translation="MNMLARTPFLAVLRKELLDTLRDRRTLIAMIVVPLLLFPVLMVG
                     MAKFTQSLAEEARDKVLTVAVADPKNDSGLADHLDSQPGIDIELTRDVSQLASRIAAE
                     EIDAAIVLDPTFRRDVDALGPGGLTLLYKSGDDLDIQKKRVLDALDSFEQGLLDRRFA
                     AMGVSPNIVRAVKVQEHDVASPRERLGKLIGGMLPYMFILFCFTGSMYPAIDLAAGEK
                     ERGTLETLLTAPVHRRTLVLGKFAVVAFAGIASALIGMIGLYVSVRQGIEGIPPEILD
                     AITQVLAPGAIALVFALLVPLSMFFAALLLMLSVYARSYKEAMSIISPLMIVVIVPAA
                     IALAPGIRLTTVTALIPVLNVSLATRELLAGTAEPGLVALVFISLVLLAAGSLWACTR
                     WFAREDIVFRS"
     gene            8076..9050
                     /locus_tag="VGB85_17705"
     CDS             8076..9050
                     /locus_tag="VGB85_17705"
                     /inference="COORDINATES: protein motif:HMM:NF025523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YndJ family transporter"
                     /protein_id="HEY0135928.1"
                     /translation="MNAASLARWTPLLGLVVWAGLVAARAPRLADEATLAELLVSFVP
                     LVLVPLVLPLAVAGDMSREPGRWARALALAHAVGALAVAVGVLLPTGGAASLALAGLW
                     LAFTGLAALHGLHRFRTRARLSLAELVIDLALLWLPGAAVWLLVYRGELVLGGFGGLA
                     ALLTASHFHAAGFATLMMTGLLGRALAGITAPVASTIYRLTAPALLLAFPLLAAGIGG
                     AIRPLELAGAGLYAVFLPVLAALQIRAAIGTRGPTLWRGLLVLSALAVLFATAFAARF
                     AMQGFYGAAVPISTMLRYHALVNMLGFLALGLLAWSRLRPAPLAGPAK"
     gene            complement(9056..9772)
                     /locus_tag="VGB85_17710"
     CDS             complement(9056..9772)
                     /locus_tag="VGB85_17710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135929.1"
                     /translation="MILRTISYTFALVLACGPEKDAGVETSSTGETQTASTGASDPTE
                     ATSDPTSSTTGVASTGDVVTGSSSDSTSSTTGGMESTGATDGTTGELMPGACRSDADC
                     SDDDQESCFAPGQSNCGPCQVPEMPCFGEGSCGADSVCVPFVAACACDPGEGMCAPKC
                     EADSCGRGATCNAMTGICEPFTCSGDGVTCPPLFECVAGSGGDECQRLACETDAECGG
                     APCVEGRCHEGFGVCQPPAP"
     gene            9844..>10129
                     /locus_tag="VGB85_17715"
     CDS             9844..>10129
                     /locus_tag="VGB85_17715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135930.1"
                     /translation="MSSPIPHNWQVPESFRERMGSQAGKQRIMTHGGHLLVILHAVPD
                     PATPEKREARLFWRSPDGTWLGSGGGAPGIASLRAHVEGFIEVANKLEADA"
BASE COUNT         1514 a         3673 c         3337 g         1605 t
ORIGIN      
        1 atctgttcga cgagcgaccc gagacctaca agacccgctc ggccggcctg gggatcgatc
       61 tgcagccata cctcgacgcc ggccggctgc tcctgcgtca gctcgatccc ggcgagatcg
      121 cccccggaga gttcgcccag caggtccggg cggccgtcga ggagggccag gcgaaggtgg
      181 tcgtgctcga cagcatcaac ggctacttca acgcgatggg caccagcgac atactcgtcc
      241 cgcagctgca cgagctcctg accttcctca gtcgcagcgg ggtcttgacg ctggtcgcgg
      301 gctcgcagga gggatttatg agcatcggct cgcaaggcgg cgtcgacatc agctacctgt
      361 cggacacgat cgcggtgctc ggctacttcg agcatcgtgg cgagatccgc cgctgcatca
      421 cggccgtcaa gaagcgccac ggcaagcacg gcacgatgat ccacgagctg acgttcgacg
      481 ctggcggcgt ccacgtcggc gaagtgctgc gcaagtaccc taacctgctg gtcggcagcg
      541 ccgggccgtc cgtgagacgc gaggacggga ttgacggcga ctgaccccca tggcgtcgtc
      601 gtgctcgccc cgctcgggcg tgatgcggcg gtcgtcggcc agctccttgc cggggccgga
      661 atcgtcagca ccgtggtccg cgatgccagc gcgctgatca cccggctggc gaacgacgtt
      721 ggcgccgtgt tggtgaccga ggaggccctg acccacgacg gcgcgaccgg actggcgaca
      781 gccttgcagg cccagccctc gtggtccgat gtcccggtgc tgttgttcgc aaccggagac
      841 cgccaggaca cggcctggca gtccgagatc ttgcgggcgg ccggaaacat cacggtactc
      901 gagcgaccga tcgccggcct gactctggtg accgccgtgc aatcggcgtt gcgggcccgc
      961 cggcgccagt acgagctgcg cgacgtgctc gcctcggagc agcgcgcccg gctcgcggcg
     1021 gaggcggcga cacggatcaa ggacgaattc ctggcgaacg tgtcgcacga gctgcgtact
     1081 ccgctgagcg cgatcctgct gtggggcaac ctgctcggca gcgggcgggc cgggcaagac
     1141 gacgcgttgt cggcgatcga gcgcagcgcg caagcccagt cgaagctcat cgaggacctg
     1201 ctcgacgtgt ctcggatgct ggccgggaag ctgcgcgtac ggctgcgtca ggggcagctc
     1261 gcgcccgtgg ccagggcggc ggtcgaggtc gtgcagccga tggccgaggc caagggggtt
     1321 cggctggcgg cgcacatcga cccgggagcc gggaccgtcc tcatcgatcc cgatcgtatg
     1381 cagcaggtgc tgtggaacct gctgagcaac gcgctgaagt tcaccccgac cggcggcgag
     1441 gtgatgatcc ggctgtctcg cctcggcggt caggtgcgag tccgcgtgtc cgacaccggc
     1501 atcggtatcg gcgcagagtt catgccgcac ctgttcgaac gcttcggcca ggcggacacg
     1561 acgagcaccc gcagcaaggg tggcctcggc ctcggcctgg cgatcgcccg ccagctggtc
     1621 gagctgcatg gcgggacgct gcgcgccgat agccgaggcg aaggccaggg cgcccacttc
     1681 acggtcgatc ttccggtgct ccgcgccgac gccgaagtcg ccggtcctga cgcgacctcc
     1741 gggccgctgc gggtgttgcc cgggttacgc gtgctcctgg tcgaggacga gcccgacacc
     1801 cggcgcgcgc tggccctcgt actcgagagc gcgggcgccg aggtgaccgc cgtcgactcg
     1861 gccgctgccg cgcgcgcggt gctggcgagc ctcaccgcgg cacgtcgacc gggagtgctg
     1921 gtcagcgaca tcggcctgcc cggcgaggat ggcatcatgc tgatccgcgc gatccgtgcc
     1981 gaagaagcgc gcctcggtga gacaccgttc cctgctctgg ccgtctcggc atactccagc
     2041 gaggccgcgc gcgagcaggc gctcgccgcc ggtttcgacc gctacctcgt caagccgatc
     2101 ctgccggacg cgctggtggt tgcgatcctc gcgctcgcac cgcctcagcc gctgctgcca
     2161 ccgctatcac cgctgtcacc gctgccaccg ctgccaccgc tgtctgatgc gtgagcgggt
     2221 gggaggtcac tggcttcact cacctggctt cacgagccgg cgacgccgtg ctcgagggca
     2281 tagcgcgtca actcggcggt gctgtgcagc cggagcactt gcatgagtct gctcttgtga
     2341 aactcggcgg tctttcgcga aatgtgcagc gcctcggcga tctgccgggc tgtgcggccc
     2401 tcggcgacca attgcagcac ttcacgctgg cgcgtcgtca ggggatccgg agccggagcc
     2461 tcgtccagga cgtcctcgag tgtgaggctc aagcccgggg acagatagcg ccggcctgcg
     2521 atgaccactc ggatcgcgct cagcagttcg cgggaggccg agcttttggt cacatagccg
     2581 accgcgccgg cagcgaaggc ttcgcggaca taactcctct cgacgcggct cgtcacgaag
     2641 acgagccggc tctccgggca aagtccaccg agctggcgcg ccgcctccag gccatttagc
     2701 cccggcattg cgatgtcgat gaggatgata tctggctgca gtcgttcggc ggcttcgagc
     2761 aagagtcggc catccgatac ggcgtcgagc acccggcact ccggggacag cagcaggcgc
     2821 aatccctcgc gcacgagcgt gtgatcttcg gcgatcaaga cgcttggcac atcgtccagg
     2881 ggcaggcgac gaacctcatc gctgcccgct tcgactggac cgctcggtac attgggcgcg
     2941 gggatcatgt cgacattgtg tttctcgatc gggcggcgcg ccaaggaccc cctggctcgt
     3001 gctctggtga ccggccaaag gccaggacga cacataccca caagcgtcca cataggtgcg
     3061 cagggggcgc ttctgtcgac ccggaatacc gtatccgtcc gaacaggcca taccgaggct
     3121 acgcggtcat gacgccggtg cgggcagcag acggctctgg tccagcgtca ggctgccagc
     3181 acggacagtc gcccgtcgcc ctcgccctcg ctcgctgcgt tgaccacctg acgatggtcg
     3241 atactcacgc agcagtccga cgcactcacg ggcaagtgag gagtgaacgc cttcgtgatc
     3301 gatcgcgcga gcgccgtgct gtggcgctcc cacccgctcg acagcgcgag cgcgaaaact
     3361 tcggtgcgag cattcgtatc gaggtgcgac agccagcgac gcaccagccc ctcggtcgct
     3421 gcccaggagg cattcacgcc ctcgatgcgc acggccgtgg tgaggcggac gatcgcccga
     3481 tcgatgcatt gcgcgaactc ctcgagcgac agcgcgtcga actgccgcag ggcgtgatag
     3541 ccctgattca cagcgctcat gtatttcttc ataaccgggc caacgccagc cgtcgcctgg
     3601 gaaaatcgcg gagaatcttc tggtcgtcgc tgggagccgt gacctcacgc gactcgccgc
     3661 cgcgtcggaa gcggcatgct cgggggatct taccaggctt cggagaacgg tctcggagcg
     3721 cactgtgaag cgatgtcgac ttccaacgac ggcttagcct tgcgacccga tctcgccgat
     3781 ctcgccgatc tcgccgatct cgccgatgct ggcgagcacc tgcgggtcgc cgcggaggcg
     3841 cggcagttga ggatgttcct cgacagcgtc caggactatg ccttcatcag cttcgatctc
     3901 gaccgtcgag tcgtaggatg gagccgcgga gccgagcgca tcctcggcta ccaggaggcg
     3961 gacgtgctcg gcctccccgg cagcgtcttt ttcacccccg aggacgtcgt gcgagaggaa
     4021 gacgaaaaag agttcgcgct cgctcgcgag gtcggccgcg ccgaggacga acgctggcac
     4081 ctccggcgcg atgggtcccg cttctggggt agcggcgtga tgaccccgtt ggtcgacgtt
     4141 gcgggccggt tgcgcggcta cagcaaggtg atgcgggaca acaccgcacg caggctggca
     4201 gacgcgcgcc tgcgcgacag cgaagagaga ctacgcctgt tctccgagaa cgtcggcgac
     4261 tacgccctgg taccggtgga cctcgagggg atggtcgtgg ggtggaaccc cggggccgcg
     4321 cgcatcttcg ggtacaccgc ggatgagatc ctcggacaac actgcgcccg ctttttcacg
     4381 cccgccgacg tcgcgtgcgg cgagccggac agggactttc gccgagcgat cgccgaggga
     4441 aagtccgagt acgagcgcga gatggtgcgc aaagatggca gcgtcttctg ggcccagtgg
     4501 gtcaccacgc cgatccgcga cgagaaaggc cagctgcgcg gcttcgccaa ggtcttgcag
     4561 gacaccaccg accgcaagct cgcccaggaa cgacttcgca gccaggtaca atccacgggc
     4621 gaggccctcg accgcacgaa ggaggagctc cgggccctgg ctggcagtct gctgagcgcg
     4681 caggaggacg agcgacgacg catcgcacgc gagctgcacg acgacctgtc ccagcggctc
     4741 gccggcctgg cgaacaacct cgcggcgctg caagatgcgc tcccggacga gcccgccagg
     4801 ctccggacgg cggtgcaagg ggtcgagcac gctgtcggcc tgctcgccag cgaggtgcgc
     4861 cagctgtcgc accgactgca cccgtcgatc ctcgatgacc tcggactcgg ccccgcgatc
     4921 cgtcggctcg tcgaggactt tcaggtcggc cgcgccgagc cggccacctt catcaagcac
     4981 accctgcccg agcagatccc acgcgacgtg gccaccgcgg tctatcgcat cacccaggag
     5041 gcgctgcgca acatcaccaa gcacgggaac aacggactgg tgcgcgtcga actcggcggc
     5101 gaggcgagcg ctctgcgcct cgtcgtcagc gacaccggac ccggcttcga tccggccatg
     5161 gtgcgcggcc ggggcggcct ggggatcatc agcatgcaag agcgtgccgg cctggtcggc
     5221 ggcaaccttc gtctgatcag ccgacgcgat cacggcacca cgatcgaagt tcgcgtgccc
     5281 ctggcgccga cctgagcgca tctgcgacat ccgacgaacc ctcggcagag ctgaccaggg
     5341 ctttcggcat ttcacccccg ggcagacggc aagctgacca atggcgagac aaactgctgc
     5401 ccctccagcg ccgcatgcac ggctgcgatg aggtgcctga acaacgaagt cttgatgacg
     5461 tagccatgcg caccggcttc cagggcggcc tgcaaatact cgggttcagt gtggttcgtg
     5521 atgaacacga cgcctactcc ggggtatagg cgcacgagcc gcgtcgcagc ctcgatgccg
     5581 ttgagccggg gcatggagat gtcaaggacg accgcgtcgg gcggcgcacg tgctgccgct
     5641 tccagcaaag cctgaccgtc cgcgacagcg tcgatgatgt cgaattcgtc gttcaaaagt
     5701 attcgtaatc cgtcccggac ctgcgcgtga tcttcggcga tcagcaaccg agggcgagag
     5761 ttgattgaca tggcgtcatc cttgacagga tttctggtgg cggccactgg taaaagtacc
     5821 ttctcggcgc cgctcatttg ggaacatggg gaagccggcg cgtcgacccg agccgcggca
     5881 agcgcggccc cgtcgaggcc cgagaacagc cacccgcgat cttcgtcggc gtggaagacg
     5941 tcggcgacgc cgtcgcgctc gaggtcgtgg accaccgagg ccggcggagc ggtctccgag
     6001 atcggcatct gccgcagcac cgcgccgtcg ccctcgaaga cgacgtcgga ccttcgtcgc
     6061 cggtcagggc cccgcccgtt cggtgtactc tccggccgtc ctcatgatca cggtccagca
     6121 cctcgccaag cgcttcactc tctcgcggca gcagcagcgc acgctcgggg accaccgcaa
     6181 gcccgggctc gtcggcaaga agcagctgca ggcggtcgac gatgtcggct ttcactgcaa
     6241 gcccgggcgg atcttcgcgc tgctcgggcc caacggcgcc ggcaagatcg agatcgccgg
     6301 cgtcgacgcg ctcgccgacc cggtcgccgc gcggcgcaag ctcggctttc tgaccggctc
     6361 gaccaagctg tacgagcgcc tgaccgccga ggagacgctg cgctactacg ccgcgctcgc
     6421 cggggtcgcg gaggccgact accgccgccg ccgcgacgcc ttgttcgcgg cgctcggcat
     6481 cgacgccttc gccgaccgcc gcgtcggcaa gctgtcgacc ggcatgcaac aaaaggtctc
     6541 gatcgcccgc acgatcatcc acgacccgga ggtcctcgtc ttcgacgagg ccacctccgg
     6601 cctcgacgtc atcgccgccc gcgcgatcat ccagtttatt cgtcagtgcc gcgccgacgg
     6661 caagacggtc ctgttctcga cccacatcat gagcgaggtg acgatgctcg ccgacgacct
     6721 ggcgatcgtg cacggcggca agctgatcta cagcggcacc ttcgccgact tcaccgccgc
     6781 gtccggtcag tcctcggtcg aggaagaatt cgtgcgccgc atcgaggcgt cggaggcctc
     6841 cgtatgaaca tgctcgcccg cacccccttc ctcgccgtgc tccgcaagga gctgctcgac
     6901 accctgcgcg accgccggac gctgatcgcc atgatcgtcg tgccgctgct gctgttcccg
     6961 gtgctcatgg tcggcatggc caagttcacc cagtcgctcg ccgaggaggc gcgcgacaag
     7021 gtcctgaccg tcgccgtcgc cgaccccaaa aacgacagcg gcctcgccga tcacctcgat
     7081 tcacagcccg gcatcgacat cgagctcacc cgggacgtct cgcaattggc ttcgcgcatc
     7141 gccgcggagg agatcgacgc cgcgatcgtg ctcgacccga cctttcgccg cgacgtcgac
     7201 gctctcggcc ccggcgggct gaccctgctc tacaagtccg gcgacgacct cgacatccag
     7261 aagaagcggg tcctcgacgc cctcgacagc ttcgagcagg gcctgctcga ccgccgcttc
     7321 gccgcgatgg gcgtcagccc gaacatcgtc cgcgcggtca aggtccagga gcacgacgtc
     7381 gcctccccgc gcgagcgcct cggcaagctg atcggcggca tgctgcccta catgttcatc
     7441 ctcttctgct ttaccggcag catgtacccg gcgatcgacc tcgccgccgg cgagaaggag
     7501 cgcggcaccc tcgagaccct gctgaccgcc ccggtccacc gtcgcaccct cgtgctcggc
     7561 aagttcgccg tcgtcgcctt cgccggcatc gcctcggcgc tgatcggcat gatcggcctc
     7621 tacgtcagcg tacgccaggg catcgagggc atcccgcccg agatcctcga cgcgatcacc
     7681 caggtcctcg cgcccggcgc gatcgcgctg gtgttcgcgc tgctcgtgcc gctgagcatg
     7741 ttcttcgccg cgctgctgct gatgctctcg gtctatgcgc gcagctacaa ggaagcgatg
     7801 agcatcatca gcccgctgat gatcgtcgtc atcgtccccg ccgcgatcgc cctcgccccc
     7861 gggatccgcc tcaccaccgt caccgccctc atccccgtcc tcaacgtctc gctcgccacc
     7921 cgcgagctgc tcgccggcac cgccgagccc ggcctcgtcg cgctggtgtt catctcgctg
     7981 gtgctgctcg ccgccgggag cctgtgggcc tgcacccggt ggttcgcgcg cgaggacatc
     8041 gtgttccgct cgtgagcagc tggtaagctg cgccggtgaa cgcggcttcg ctcgctcgct
     8101 ggacccccct gctcggcctc gtcgtctggg ccggcctcgt cgccgcccgg gcccctcgcc
     8161 tcgccgacga ggccaccctc gccgagctgc tcgtcagctt cgtgccgctc gtgctcgtcc
     8221 cgctggtcct gccgctggct gtcgccgggg acatgtcccg ggagccaggt cggtgggctc
     8281 gcgcgctcgc tctggcccac gccgtcggcg cgctcgcggt cgcggtcggc gtcctgctgc
     8341 cgaccggcgg cgccgcctcg ctcgcgctcg ccgggctgtg gctggccttc accggcctcg
     8401 ccgcgctcca cggcctgcac cgcttccgca cccgcgcccg cctgagcctc gccgagctgg
     8461 tgatcgacct cgcgctgttg tggctcccgg gcgccgcggt gtggctgctc gtatatcgcg
     8521 gcgagctcgt gctcggcggc ttcggcgggc tcgcggccct gttgaccgcc tcgcacttcc
     8581 acgccgccgg cttcgccacc ctgatgatga ccggcctgct cggccgcgcc ctcgccggca
     8641 tcacggcccc cgtcgcgtcg acgatctacc gcctcaccgc gccggcgctg ctgctcgcct
     8701 tcccgctgct cgccgccggg atcggcggcg cgatccgccc gctcgagctc gccggcgccg
     8761 gcctctatgc cgtcttcctg ccggtgctgg cggccctgca gatccgggcc gcgatcggca
     8821 cgcgtggccc gacgctgtgg cgcggcctgc tggtgctgtc ggccctcgcc gtcctgttcg
     8881 ccaccgcctt cgccgcgcgc ttcgccatgc agggcttcta cggcgccgcg gtcccgatct
     8941 cgacgatgtt gcgctaccac gcgctcgtca acatgctcgg ctttttggcc ctcggcctgc
     9001 tcgcgtggtc acgcctgcga cccgcgccgc tcgcgggtcc ggcgaagtag ttccttcatg
     9061 gcgccggcgg ctgacacacg ccgaagccct cgtgacagcg gccctcgacg cacggcgcac
     9121 caccgcactc cgcgtccgtc tcgcaggcca ggcgctggca ctcgtcgccg ccgctgccgg
     9181 ccacgcactc gaacagcggc gggcaggtga caccgtcccc ggagcaggtg aacggctcgc
     9241 agatccccgt catcgcgttg caggtcgccc cgcgcccgca gctgtccgcc tcgcacttcg
     9301 gcgcgcacat gccctcgccg gggtcacacg cgcacgcggc cacgaagggc acgcacaccg
     9361 agtccgcgcc gcagctgccc tcgccgaagc acggcatctc gggcacctgg cacgggccac
     9421 agttcgactg gccgggcgcg aagcaactct cctggtcgtc gtcggagcag tcggcgtcgc
     9481 tgcggcacgc gccgggcatc agctcgcccg tggtcccgtc cgtcgcgccg gtcgactcca
     9541 tgccgccggt cgtcgagctg gtcgagtcgg agctgctgcc ggtgacaaca tcgccggtgc
     9601 tcgccacgcc agtggtcgag gatgtcggat cgctcgtcgc ctcggtggga tcactggcgc
     9661 cggtgctcgc cgtctgcgtc tcgcccgtac tcgatgtctc gacgccggcg tccttttccg
     9721 ggccacaggc gagcacgagc gcgaaagtgt aagagatcgt ccgtaagatc attgcgccag
     9781 cctaacaccg tgatccgccg ccatcctctt ttccccgcgc ggcgcccggc ggatactccc
     9841 cgcatgtcga gcccgatccc gcacaactgg caggtccccg agagcttccg cgagcgcatg
     9901 ggcagccagg ccggcaagca gcgcatcatg acccacggcg gtcacctgct cgtgatcctg
     9961 cacgcggtcc ccgaccccgc gacgcccgag aagcgtgagg cccggctgtt ctggcgcagc
    10021 cccgatggca cctggctcgg cagcggcggc ggagcgcccg gcatcgccag cctgcgcgcg
    10081 cacgtcgagg gcttcatcga ggtcgccaac aagctcgagg ccgacgccg
//
LOCUS       DASWNJ010001048         1871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_267375, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001048 DASWNJ010000000
VERSION     DASWNJ010001048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267375"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..482
                     /locus_tag="VGB85_17720"
     CDS             114..482
                     /locus_tag="VGB85_17720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135931.1"
                     /translation="MRSLHRVYLGFTFVAGLVSSACSSSNVGKACDKDSECGGDGLIC
                     DVHDGKGTCQEPHGHEAGDDDTAHHASEDTAHHATEDTAHHATEDTAHHTTGTSTEDT
                     SHHDTEDTAHHDTEDTSHDH"
     gene            complement(503..1582)
                     /locus_tag="VGB85_17725"
     CDS             complement(503..1582)
                     /locus_tag="VGB85_17725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135932.1"
                     /translation="MPRPHLLLALLLGACGDAASATSATNSTTTTGITGASQPTSDPS
                     TSTGEATPTTGTAGSATGSTSTGDGSDGPKLDLGMPDFGGRDTDGGCVAVDLLFVIDN
                     SPSMGPYQEALGAAFPMFVDEMFAALPPGTDLHVGITTTSFYSGNCSESTNNCKSTSS
                     EAEILEHYTPPTDGDNGENGGQGRLFDWEDMRFFAADTAGDPGPLKTWFTGAATAAGE
                     VGCSFEMAAAGAGWSADPANAGSNAGFLRDEGAVLLVFVLSDEPDKSPEPVSQWVDKL
                     VAAKQGCGGLQCILAAGLVSDFCHDNAADTTLKTFLESFSEPPIIGSIGSVFPNDPPP
                     DYTGVVGSALAQVIGQKCDELQPPG"
BASE COUNT          258 a          602 c          723 g          288 t
ORIGIN      
        1 ggcagcatcg gggggactgg cataacgcga ggtgggcggc ggtggaggcg gttctggtgc
       61 gtgcgacagc gggtcgcagt cgcgcgccgc ggcggtcggg ttatagggcg agcatgcgat
      121 cgcttcaccg cgtttatctc ggtttcacct tcgtcgctgg cctcgtgtcc tccgcttgca
      181 gctcgtccaa cgtcggcaag gcgtgcgaca aggactcgga gtgcggcggc gacgggctga
      241 tctgcgacgt ccacgacggg aaggggacgt gccaggagcc gcacgggcac gaggcggggg
      301 acgatgacac cgcgcaccac gccagcgagg acacggcgca ccacgccacc gaggatacgg
      361 cgcaccacgc caccgaggac acggcgcacc acacgaccgg gacctcgacg gaggacacct
      421 cgcatcacga cacggaggac accgcgcatc acgacacgga ggacacctcg cacgatcatt
      481 gagtcggcgg ggtgttcctc ggtcagccgg gcggctggag ctcgtcgcac ttctggccga
      541 tgacctgcgc gagcgcggag ccgacgacgc cggtgtagtc cggcggcggg tcgttcggga
      601 acacgctgcc gatgctgccg atgatcggtg gctccgagaa cgactcgagg aaggtcttga
      661 gggtcgtgtc ggcggcgttg tcgtgacaga agtcgctgac gaggccggcc gcgaggatgc
      721 actgcaggcc gccgcagccc tgcttggcgg cgacgagctt gtcgacccac tgcgagaccg
      781 gctcggggga cttgtcgggc tcgtcgctga gcacgaagac cagcagcacg gcgccctcgt
      841 cgcgtaaaaa gccggcgttg ctgcctgcat tggcggggtc ggcggaccag ccggcgcccg
      901 cggccgccat ctcgaacgag cagccgacct cgccggccgc cgtcgcggcg ccggtgaacc
      961 aggtcttgag cggcccaggg tcgccggcgg tgtcggcggc gaagaagcgc atgtcctccc
     1021 agtcgaacag gcggccctgg ccgccgttct cgccgttgtc gccgtcggtc ggcggggtgt
     1081 agtgctcgag gatctcggcc tcgctcgagg tgctcttgca gttgttggtg ctctcgctgc
     1141 agttgccgct gtagaaggag gtcgtcgtga tgccgacgtg caggtcggtg cccggcggca
     1201 gggcggcgaa catctcgtcg acgaacatcg ggaaggcggc gccgagcgcc tcctggtacg
     1261 gacccatcga cggcgagttg tcgatcacga acagcaggtc gacggcgacg cagccgccgt
     1321 cggtgtcgcg gccgccgaag tcgggcatgc cgaggtcgag cttgggcccg tcgctgccgt
     1381 cgccggtgct cgtgctgccg gtggccgagc cggcggtgcc ggtggtcggg gtggcctcgc
     1441 cagtgctcgt ggaggggtcg ctcgtcggct gcgaggcgcc ggtgatgccg gtggtggtcg
     1501 tcgagttggt cgccgaggtg gccgaggccg cgtcgccgca ggcgccgagg aggagggcga
     1561 ggagcaggtg cgggcgtggc atcccgtcgc ttctacgccc gcgagcgcgt ccgcgccaac
     1621 cgctcgtcgc catcggcgat gcggtcgagc agctcggcga gcaggtgccg ctcgccgctc
     1681 gagagcgcgg aggtgttggc gaagttcctg gctcaaaagc ccgcaggctg accgcgacgt
     1741 ttcgcgcttg acttcgcgcg ccgctcgggc gcgataatgg gcggtgctct ctgcgcctgt
     1801 ccttcgcgtc ctgaccgggt ttctcgtgct gctcgcctgc gatccgggcg aggaggcgcc
     1861 ggtcgtgcgg g
//
LOCUS       DASWNJ010001049         1531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_158857, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001049 DASWNJ010000000
VERSION     DASWNJ010001049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158857"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="VGB85_17730"
     CDS             complement(<1..1222)
                     /locus_tag="VGB85_17730"
                     /inference="COORDINATES: protein motif:HMM:NF036933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HEY0135933.1"
                     /translation="MHRTLAVPSPLLFTALLLAACGDNGAGTGDVSTGPLTGTETGSS
                     PTSTPTSTPATDTTPTSDPSTDATDSGMSASGTMATMATMATDPATDTNPTTVTTTTN
                     PTSATDTDPGTTGDTTTGAVSGTTSTSGSTGDDTTGGDDSTTGEDPNFGEIPETCEQA
                     ILSKSTVGCLFYAIDMDSHDLAELGQYAVAVANVQKMSPANVTIERKLNNVWEVIAGP
                     TQVGTLNLSVFNLPDKHTDDSQLGKGFAYRVKSDVPVIAYQFNPVDGQNSFLSDAAML
                     YPVTAWDHINHAITFTSTTDNTNTAQHSYASAVAFADGTKITVTPSVATKAGAGVPAG
                     QPGVPFEVTLDEGDVLSVAVNQIGTSLSGTIFESVKDTPFGMFAGLECALIPADKCCC
                     DHMEEQISGVRLWGK"
BASE COUNT          195 a          448 c          610 g          278 t
ORIGIN      
        1 tcttgcccca caggcgcacg cccgagatct gctcctccat gtggtcgcag cagcatttgt
       61 cggcggggat cagcgcgcac tcgaggccgg cgaacatgcc gaagggggtg tccttgacgc
      121 tctcgaagat cgtgccggac agcgaggtgc cgatctgatt gacggcgacg ctgagcacgt
      181 cgccctcgtc gagggtcacc tcgaagggca cgccgggctg gcccgcgggc acgccggcgc
      241 cggccttggt ggcgacggag ggggtgacgg tgatcttggt gccgtcggcg aaggcgaccg
      301 cgctggcgta gctgtgctgg gcggtgttgg tgttgtcggt cgtcgaggtg aaggtgatcg
      361 cgtggttgat gtggtcccag gcggtgaccg ggtacagcat cgcggcgtcc gacaggaacg
      421 agttctggcc gtcgaccggg ttgaactggt aggcgatgac gggcacgtcg gacttgacgc
      481 ggtacgcaaa gcccttgccc agctggctgt catcggtgtg cttgtcgggc aggttgaaga
      541 cgctgaggtt gagcgtgccg acctgggtcg ggccggcgat gacctcccac acgttgttga
      601 gcttgcgctc gatcgtgaca ttggccggcg acatcttctg cacgttggcg acggcgacgg
      661 cgtactggcc gagctcggcg aggtcgtgcg agtccatgtc gatcgcgtag aacaggcagc
      721 cgacggtgct cttggacagg atcgcctgct cgcaggtctc ggggatctcg ccgaagttgg
      781 ggtcctcgcc ggtggtcgag tcgtcgccgc cggtcgtgtc gtcgccggtg ctgccgctgg
      841 tcgaggtcgt gccggagacg gcgccggtcg tggtgtcgcc ggtcgtgccg gggtcggtgt
      901 cggtcgcgga ggtcgggttg gtggtcgtcg tcacggtcgt cgggttggtg tccgtggccg
      961 ggtcggtcgc catcgtcgcc atcgtcgcca tggtccccga ggcgctcatg ccggagtcgg
     1021 tcgcgtcggt gctcgggtcg ctggtcgggg tggtgtcggt ggccggcgtg ctcgtcggcg
     1081 tgctggtcgg cgaactgccg gtctcggtgc ccgtgagcgg gccggtcgag acatcgccgg
     1141 tgccggcacc gttgtcgccg caggcggcga gcagcagggc tgtgaaaagg aggggggacg
     1201 ggacggcaag ggtgcgatgc atcgggttaa ttcctcgggg tctgcagcgt cgagggtccc
     1261 aaaacctgcg acgtcgtgtc caccgacgcg gtcgaattga gcggtacttg cgcgcgccct
     1321 cgggtccctc gcggaccggg gaatcccgcc gagcggaaag cgctgctggc gggctcgtgg
     1381 tcgaggcgag gggagagcta catcactgtt ctgaccggtc aagaccgcgc aaatcggctt
     1441 cggcgcagcc gcgcggggga gcgacgcacg tcacgcccgg cggcggcgat cagtgccggg
     1501 cgtggcactg cgcccatact tgggcgacgc g
//
LOCUS       DASWNJ010001050         1519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_158874, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001050 DASWNJ010000000
VERSION     DASWNJ010001050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158874"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1006)
                     /gene="asd"
                     /locus_tag="VGB85_17735"
     CDS             complement(173..1006)
                     /gene="asd"
                     /locus_tag="VGB85_17735"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HEY0135934.1"
                     /translation="MIASSPDWGASAASKALGAAARARLPRRLSQAMVSAYVRYFGVD
                     LEDVDAANIGDGYASFNAFFTRQLRAGARPVAEDATTIVAPSDGLLRSVDAIEHGTTI
                     AAKGHAYSVGELLADEAAAEQYVGGHATTIYLHPRDYHRVHCPCDARVRSVTAVPGRL
                     LPVTAAAMLREPQLFAVNERLIHHLDTDFGAMAVVMVAAFGVGHMSCAYRPVVTHPRK
                     IQRTTCEPPARLRKGDELGVFHLGSTVVLLTQRGVRPTIDELPVAVRMGQALFEGSET
                     R"
     gene            complement(1170..>1519)
                     /locus_tag="VGB85_17740"
     CDS             complement(1170..>1519)
                     /locus_tag="VGB85_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase catalytic subunit"
                     /protein_id="HEY0135935.1"
                     /translation="DVRRRRPRIATTYGGLSGPAIKPIALRMVHQVARAFPDLPICGM
                     GGVLSGEDALEFLMAGARCVQVGTANFAEPGACLRITHELRAVMAEVGVARVADAVGC
                     LEAWPAEAALSGQ"
BASE COUNT          202 a          523 c          600 g          194 t
ORIGIN      
        1 ggacgcgggg tcgcgcgacc ggtcagcgag ggcatgctga cgccggtcgg cagcgcgacg
       61 agcatcgagc gcaggcgatc gtcgatcgcg tcgagcgact cgccggcgcg gacgcggccg
      121 cgcggcacgc tggtgaagtc cagcagccac tcctcgggtg agacgtgcca ggtcatcggg
      181 tctcgctccc ctcgaacagg gcctggccca tgcgtaccgc caccggtagc tcgtcgatcg
      241 tcggtcgcac gccgcgctgg gtcagcagga cgacggtgct tccgaggtga aacacgccga
      301 gctcgtcgcc cttgcgcagg cgcgccgggg gctcgcacgt ggtgcgctgg atcttccgcg
      361 ggtgggtgac gaccgggcgg taggcgcagc tcatgtggcc gacgccgaag gcggcgacca
      421 tgacgacggc catcgcgccg aagtcggtgt cgaggtggtg gatcaggcgc tcgttgacgg
      481 cgaacagctg cggctcgcgc agcatcgccg cggcggtgac cggcagcagg cgaccgggca
      541 cggcggtgac gctgcggacc cgggcgtcgc atgggcagtg cacgcggtgg tagtcgcggg
      601 ggtggaggta gatcgtggtc gcgtggccgc cgacgtactg ctccgcggcg gcctcgtccg
      661 cgagcagctc gccgacgctg taggcgtggc ccttggcggc gatcgtggtg ccgtgctcga
      721 tcgcgtcgac gctgcgcagc aggccgtcgc tcggggcgac gatcgtggtc gcgtcctcgg
      781 ccaccggccg cgcgcccgct cgcagctggc gcgtgaagaa ggcgttgaag ctggcgtagc
      841 cgtcgccgat gttggcggcg tcgacgtcct cgaggtcgac gccgaagtaa cgcacgtagg
      901 ccgagaccat ggcctgactc aggcgccgcg gcagacgggc ccgtgcggcc gccccgagcg
      961 ccttgctggc ggcgctcgcc ccccagtcgg gcgaggaggc gatcacggcg gccgcgaggc
     1021 gggctctcca gggaattgcg cgatctggca tccttgcagg acagcgtatc caggcgctgc
     1081 gcgggccgca agcgcgacgg gaggcgcgga caggccgtgg cgcgccccgc cgcgcgcgct
     1141 gttgcgcctg ggggccggca gcacgcgcct cactggcccg acagcgcggc ctcggcgggc
     1201 caggcctcga ggcagccgac cgcgtcggcg acccgggcga cgccgacctc tgccatcacg
     1261 gcgcgaagct cgtgggtgat ccgcaggcag gcgccgggct cggcgaagtt ggcggtgccg
     1321 acctgaacac agcgcgcgcc ggccatcagg aactcgagcg cgtcctcgcc gctgagcacg
     1381 ccgcccatcc cgcagatcgg cagatccggg aaggcgcggg cgacctggtg gaccatgcgc
     1441 agggcgatcg gcttgatcgc cggaccggac agcccgccgt aggtggttgc gatcctcggc
     1501 cggcggcggc gcacgtcga
//
LOCUS       DASWNJ010001051         2426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_89368, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001051 DASWNJ010000000
VERSION     DASWNJ010001051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89368"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..1327
                     /locus_tag="VGB85_17745"
     CDS             35..1327
                     /locus_tag="VGB85_17745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02996 domain-containing protein"
                     /protein_id="HEY0135936.1"
                     /translation="MTTPAEQAFLARILAAPDDDAPRLEYADWLERRGDPRGAFIRAM
                     IALHNLGVDGDHDERLRLCAIYQPFERAWTDELCTLLGAPAGTNYFPATFSRGFIEAL
                     RVPADLLAGMSGVLFSIAPLLRSLSITQPTNSAFDHALGEALARRPELAQITSIRLFS
                     CDLDDEPVIAFVASPHLAGLRELDLAANKLGPAAVAAVLAAPFAGNLTTLQLWKNKIG
                     DEGAAVIAASAPPGLRHLSLGQNGIGPHGGLALAGSQRLAAVEEIDLGDNPIADEGVC
                     AIVGSTALAGLRRLDVHQVGLGVDGVRRLAALPGLARIEVLGIGCNDIGDAGLAALAA
                     SPHTGGLVHLDLEGSEIGDVGMQALAQARDLGRLRELGLRYNRITNVGAAALAASRCL
                     PGFIDLDLAYNDIEDEAVLMDRFDHSNLIGQGRRTEIR"
     gene            1397..>2426
                     /locus_tag="VGB85_17750"
     CDS             1397..>2426
                     /locus_tag="VGB85_17750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135937.1"
                     /translation="MRHLGLVPALLLSLAPVGCGDDVAAGSDTDTGTSTDASTSTTDD
                     TPTTGDSTIDPPTGTTGDGLCAADPDCDDDNDCTLDRCGGDGVCTVEAVVSNSCRPQI
                     TVDYPPRGATIVGKPGAPVVTVTGKVTSGAGAISTLMLNDEPVPVQKDGKFATDLLVT
                     PGGTTLDFHVEDIAGGKRRRVQSFLWSSEYRRPEVSGEQMVPHGLAFYLGQQALDDGD
                     PGTPADDIATLLGVALSSFDIAALIDPKKPITSTQGYDVYLTALSFGSTQVGLSARDG
                     GLAITADLKDVLGDLFFDCTTWTCQLAGGDSTGGLSITKLGVTVDTTIYVDAQGQVQV
                     MAMNAMANL"
BASE COUNT          359 a          952 c          772 g          343 t
ORIGIN      
        1 ccatgatacc gtaccgcggt cgaaccgacc caccatgacc acccccgctg agcaagcgtt
       61 cctcgcccgg atcctcgccg cgcccgacga cgacgccccg cgcctcgagt acgcggattg
      121 gctcgagcga cgcggcgacc cgcgcggcgc ctttattcgc gccatgatcg ccctgcacaa
      181 cctcggcgtc gacggtgacc acgacgagcg cctgcgcctg tgcgcgatct accagccctt
      241 cgagcgggcc tggaccgacg agctctgcac gctgctcgga gcgccggccg ggaccaacta
      301 cttcccggcc accttctcgc gcggcttcat cgaggcgctg cgggtgcccg ccgacctgct
      361 cgccggcatg tccggggttc tgttctccat cgccccgctg ctgcgctcgc tctcgatcac
      421 acaaccgacg aattccgcct tcgaccacgc gctcggcgag gcgctggccc gccgccccga
      481 gctcgcgcag atcaccagca ttcgcctgtt ctcctgcgac ctcgacgacg aaccagtgat
      541 cgccttcgtc gcctcgcccc acctcgccgg cctgcgcgag ctcgacctcg ccgccaacaa
      601 gctcggtccg gcggcggtcg ccgccgtgct cgccgcgccg ttcgccggca acctgacgac
      661 cctccagctg tggaagaaca agatcggcga cgagggtgcc gcggtgatcg ccgcctcggc
      721 gccacccggg ctgcgccacc tgtcgctcgg ccagaacggc atcggtcctc atggcggcct
      781 cgcgctcgcc ggctcgcagc gactcgccgc cgtcgaggag atcgatctcg gcgacaaccc
      841 gatcgccgac gagggcgtct gtgcgatcgt cggcagcacc gcgctcgccg gcctgcgaag
      901 gctcgatgtc caccaggtcg gcctcggcgt cgacggtgtg cgccggctcg ccgcgctgcc
      961 ggggctcgcg cgcatcgagg tcctcggcat cggctgcaac gacatcggcg acgctggcct
     1021 cgcggcgctc gccgcgtcgc cgcacaccgg cgggctcgtc cacctcgacc tcgagggctc
     1081 ggagatcggc gacgtcggca tgcaggccct cgcgcaggcg cgcgacctcg gccgcctccg
     1141 cgagctcggg ctccgctaca accgcatcac gaacgtcggc gccgccgcgc tcgcggcctc
     1201 gcgttgtcta cccggcttta tcgacctcga cctcgcctac aacgacatcg aggacgaggc
     1261 cgtcctcatg gaccgcttcg accacagcaa cctgatcggt cagggccgcc gcaccgagat
     1321 ccggtgactg cgacccgcgt gatcccgatt acagcgcccg cggcacgccc gccgcaccgg
     1381 gtgctatggt cgaaagatgc gtcacctcgg cctggtcccc gcgctgctcc tgtctctcgc
     1441 gccggtcggc tgcggcgacg atgtcgcggc cggctccgac acggacaccg gcacgagcac
     1501 ggacgcgagc acgagcacga ccgacgacac cccgacgacc ggcgactcga cgatcgaccc
     1561 gccgaccggg accaccggcg acggcctgtg cgccgccgac ccggactgcg acgacgacaa
     1621 cgactgcacg ctcgaccgct gcggtggaga cggcgtgtgc acggtcgagg ccgtcgtgtc
     1681 gaacagctgt cgcccgcaga tcaccgtcga ctatccgccg cgcggcgcga cgatcgtcgg
     1741 caagccgggc gcgccggtcg tcaccgtgac cggcaaggtc accagcggcg cgggtgcgat
     1801 ctcgaccttg atgctcaacg acgagccggt gcccgtgcaa aaggacggca agttcgctac
     1861 cgacctgctc gtcacgcccg gcggcaccac cctcgacttt cacgtcgagg acatcgccgg
     1921 cggcaagcgg cggcgcgtgc agtcgttcct gtggtcgagc gagtatcgcc ggcccgaggt
     1981 ctccggcgag cagatggtgc cccacggcct cgcgttttac ctcggccagc aggcgctcga
     2041 cgacggcgac ccggggaccc cggccgacga catcgcgacc ctgctcggcg tcgcgctgtc
     2101 gagcttcgac atcgccgcgc tgatcgaccc caaaaagccg atcacgagca cccagggcta
     2161 cgacgtctac ctgaccgcgc tgagcttcgg ctcgacgcag gtcggcctgt cggcgcgcga
     2221 cggtggtctg gcgatcaccg ccgacctcaa ggacgtgctc ggcgacctgt tcttcgactg
     2281 cacgacctgg acctgtcagc tcgccggcgg cgactcgacc ggcggcctga gcatcaccaa
     2341 gctcggcgtc accgtcgata ccacgatcta cgtcgacgcc cagggccagg tgcaggtcat
     2401 ggcgatgaac gcgatggcca acctcg
//
LOCUS       DASWNJ010001052         2480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_196574, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001052 DASWNJ010000000
VERSION     DASWNJ010001052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196574"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VGB85_17755"
     CDS             <1..275
                     /locus_tag="VGB85_17755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135938.1"
                     /translation="AAPAAKAPTPPDPDPSVDEGIACKVDADCPVLGCGPCTPGTPVT
                     RRRLDGPACDVNPCEDAASVCTNQVCVVHPGTHKDPAVWGTATPRP"
     gene            complement(278..892)
                     /locus_tag="VGB85_17760"
     CDS             complement(278..892)
                     /locus_tag="VGB85_17760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135939.1"
                     /translation="MKHTTTHKTATFELVWSLTVAEGEARTLELEYQLTAFEELYLAD
                     RLWDHDRAGRRIADPYGVYRFVKDGGLWLVFAQAPWRPEVRLENIYTPLCSRVRAGEA
                     RRHAVSIALPVDEYSALARNVAAETTLVQVSRVTLVLGHRLRASMDADPLPPPFESAE
                     QAGYIVDDHAMIVSSLAVDPLPVKRRTGAIARFAPPDPRPPSRP"
     gene            complement(913..>2480)
                     /gene="tssI"
                     /locus_tag="VGB85_17765"
     CDS             complement(913..>2480)
                     /gene="tssI"
                     /locus_tag="VGB85_17765"
                     /inference="COORDINATES: protein motif:HMM:NF026241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HEY0135940.1"
                     /translation="DENSSCWVRCSTAWAGRGYGQFSVPRIGQEVLVDFLEGDPDRPV
                     VVGRIYHGDNPPPYPLPDHKTRSTIKSESSPGGGGFNELRFEDRKGQEEVFLHAEKDW
                     NTVILHDLTESVGVNRSSSIGVNESVVVGTNRSVMVGSNNSTIVGNAHSVTIKQPPPP
                     RIGPPPPPIPPTGTTMSDKFFSLTSGLATVSVNGADVTIDATGTVTIHSVGNLSVRSD
                     ADLFLLGQNIIMAAAATMTIGGVDVTVNGTTLALGGKAKAELTSSGPTTVSGTPVQLN
                     GPGLFAGRVTELAPAAITTGAALVLVGGASFPLPVVKLPDGTLQIGQNITVSPGTGRY
                     EDFQNKVLRDLGIMSSTPAGAQRLANIENDPGKHNINIREYSAAEEAEYGANNSLAAA
                     TGDGAGLTYDKDGNPVPGSGSGSVISYNPDAVSGPAGHPKPADSRLFHEMGHAEHNSM
                     GIRRDDPMTGGWTDGEEYQTIQGGVNQPGGTMVPGKPTSPSENQYLEQRNYPYRRTGH
                     GKWGNPDGSPIVP"
BASE COUNT          328 a          857 c          900 g          395 t
ORIGIN      
        1 ccgcagcgcc tgccgcgaag gcgccgaccc cccccgaccc cgacccgtcg gtcgacgagg
       61 gcatcgcgtg caaggtcgac gccgactgcc cggtgctcgg ctgcggtccg tgcacgccgg
      121 ggaccccggt cacgcggagg cgtctggatg gcccggcctg cgatgtcaac ccatgcgagg
      181 acgcggcatc ggtgtgcacg aaccaggtct gcgtcgtgca ccccgggacc cacaaggacc
      241 cggcggtgtg gggcaccgcg acgccgcggc cgtgagctca ggggcggctc ggcgggcgcg
      301 gatcaggcgg cgcgaagcga gcgatcgcgc cggtgcgccg cttgaccggc agggggtcga
      361 ccgcgagcga cgagacgatc atcgcgtggt catcgacgat gtagccggcc tgctcggcgc
      421 tctcgaacgg cggcgggagc ggatcggcgt ccatgctcgc gcgcaggcga tggccgagca
      481 cgagggtcac ccgcgagacc tgaacgaggg tcgtctcggc ggcgacgttg cgcgcgaggg
      541 ccgagtactc gtcgaccggt agcgcgatcg agaccgcgtg gcgcctcgcc tcgccggcgc
      601 gcacccgcga gcacagcggc gtgtagatgt tctccaggcg cacctcgggc cgccagggcg
      661 cctgcgcgaa caccaaccac aggccgccgt ccttgacgaa gcgatacacc ccgtacgggt
      721 cggcgatccg ccggccggcg cggtcgtgat cccacagccg gtccgcgagg tagagctcct
      781 cgaacgccgt gagctggtac tcgagctcga gcgtgcgcgc ctcgccctcg gcgacggtca
      841 gcgaccagac gagctcgaag gtggcggtct tgtgcgtggt ggtgtgcttc atcgtcgtgg
      901 cctcgtctgc cgtcacggca cgatcggcga tccgtcgggg ttgccccact tgccgtgccc
      961 ggtgcggcgg tacggatagt tgcgctgttc gaggtactgg ttctcgctcg ggctggtcgg
     1021 cttgcccggc accatggtgc cgcccggctg gttcacgccg ccctggatcg tctgatattc
     1081 ctcgccgtcc gtccagccgc cggtcatcgg gtcgtccctg cggatcccca tggagttgtg
     1141 ctcggcgtgg cccatctcgt ggaacagacg agagtccgcc ggcttggggt ggcccgccgg
     1201 acccgacacg gcgtcggggt tgtacgagat cacggagccg gagccggagc cggggacggg
     1261 gttgccgtcc ttgtcgtacg tcagccccgc gccgtcgccg gtcgcggcgg ccagcgagtt
     1321 gttcgcgccg tactcggcct cctccgccgc cgagtactcg cggatgttga tgttgtgctt
     1381 gccagggtcg ttctcgatgt tggcgagccg ctgcgcgccc gcgggggtcg aggacatgat
     1441 gccgaggtcg cgcaggacct tgttctggaa gtcctcgtag cggccggtgc ccgggctcac
     1501 cgtgatgttc tggccgatct gcagggtccc gtccgggagc ttcacgaccg ggagcgggaa
     1561 cgaggcgccg ccgaccagca cgagcgcggc gcccgtggtg atcgcggcgg gggccagctc
     1621 cgtcacgcgg ccggcgaaca gaccggggcc gttgagctgc accggcgtgc cgctgaccgt
     1681 ggtcggcccg ctcgacgtca gctccgcctt ggccttgccg ccgagcgcga gcgtggtgcc
     1741 gttgacggtg acgtcgacgc cgccgatcgt catggtcgcc gcggcggcca tgatgatgtt
     1801 ctggccgagg agaaacaggt cggcgtccga ccggaccgac aaattcccga ccgagtggat
     1861 ggtgaccgtg cccgtcgcgt cgatcgtgac gtctgcgccg ttgaccgaga ccgtcgccag
     1921 cccgctcgtc aggctgaaga acttgtccga catggtggtg cccgtggggg ggatcggcgg
     1981 cggtggcggg ccgatccggg gcggtggcgg ctgcttgatc gtcacgctgt gcgcgttgcc
     2041 gacgatcgtc gagttgttcg acccgaccat gaccgagcgg ttcgtcccga ccacgaccga
     2101 ctcgttgacc ccgatcgacg acgaccggtt cacccccacg gactcggtca ggtcatgcaa
     2161 gatgaccgtg ttccagtcct tctccgcgtg caagaagacc tcctcctggc ccttcctgtc
     2221 ctcgaagcgc agctcgttga acccgccgcc cccgggcgag ctctcggact tgatcgtgct
     2281 ccgggtcttg tggtcgggca gcggatacgg cggcggatta tcgccgtggt agatccggcc
     2341 gacgacgacc ggccggtccg ggtcgccctc gaggaagtcg acgagcacct cctgcccgat
     2401 gcgcggcacc gagaactgcc cgtagccgcg gcccgcccac gcggtcgagc agcgcaccca
     2461 gcacgagctg ttctcgtcgc
//
LOCUS       DASWNJ010001053         3551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_615838, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001053 DASWNJ010000000
VERSION     DASWNJ010001053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615838"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2087
                     /locus_tag="VGB85_17770"
     CDS             <1..2087
                     /locus_tag="VGB85_17770"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein
                     kinase family protein"
                     /protein_id="HEY0135941.1"
                     /translation="GTPAYMSPEQHFGHLSGPYSDQFSFAVTLYEALYGARPFVGDSW
                     ASIKTQVESGVVPPPPPDSPVPRRLFKILLRALSTAPEKRWPTLGEMLDALEHDPQRA
                     RIRVAAMVGLLGAASAGSYAVAIARAPEADRCEAGAKEMASVWGEERRTAVARAFAST
                     GTPFAADVLQRVEERLDSYTKTWASEHRLACESNAAGLETTRLMDLRFACLGRRKVHM
                     QALLDVFTTASGEVVENAVQAVAALPGVQACADAEVLLADVAPPGDPETARKVDALRE
                     RLARGAALEGTGGYAEGLQVVQAARAEAITVGYAPLTAEAALAEGSLLTTAGRLNEAE
                     AALAEAVRLGIAQDMHAVAAEAAARRIFVIGVGLRDHPTALASQLFAEALVERASDDG
                     RLEALLLNNLGTVFALKGDDAAARTHFQKTIELLRSSGDADPLLAVVHHNLAEIDLKR
                     GNLEGAEDNSTRAHALFIALLGQRHPLVAHPLAGLGDVELKRGNPDNLVLADKRYREA
                     LALMEAGHGPEHPYLLHPLTGLGRVAARQGNVEQARRRFDRVVAIAERNEFMHPLLGE
                     ALEELGELSAAEGDGSKALASFERAVQVYGDDHELRAAAIRRAGEMAAKLKDPAPAIA
                     WFERIVASAPGPVTDAPMRLAAALALARLYAGSDRVGPVCELLREAEAALPVVDDRRE
                     RVAAGLKSCPPR"
     gene            complement(2084..>3551)
                     /locus_tag="VGB85_17775"
     CDS             complement(2084..>3551)
                     /locus_tag="VGB85_17775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135942.1"
                     /translation="AALVIVAASAAIRWPGFTQGGLASHDVGGILYNAMLLRSGGLPY
                     VDSVEWKAPGSFYVAALVADGNDIAALQVAANLVALGSLVLVMALAWRLWGRGAAVAC
                     GAVFALHDAQLDSMDANYVTWALLPQIAALALAFAARRRPVPSVMWLASGALCGVATL
                     CKQPAGIVIVPVIACALRSAGKWELRAGATVVLGFVAAHAPIGLHYLARGQLGALLAS
                     YVGADWGMAYIGHHADMPLHLAAWEGLAATVFFLALPLTLAVFATAARPRDETASATT
                     RLLWIWALAAIAGAAVGLRFYKGYFLAALPPLCLLAAAPWGMLAAGFWRRGVARWLAA
                     PVLALLVARAGVMLHATRVDRARPHDDGARKLADHIARHSEPGARIWVWGWHLWGVYA
                     YSDRLSASPIYKTPGLLTRFDDDTWRRPSTPVTLIPDSPHADRLLRDLDAARPIFITL
                     GGSVPREKFVALRRFLLTHYERDRRITVGKVEVWRRRD"
BASE COUNT          514 a         1354 c         1274 g          409 t
ORIGIN      
        1 tcggcacgcc ggcgtacatg tccccggagc agcacttcgg gcacctgagc ggcccgtact
       61 cggaccagtt cagcttcgcc gtcacgctct acgaggcgct gtacggcgcg cggccgttcg
      121 tcggcgactc gtgggcgagc atcaagacgc aggtcgagag cggcgtcgtc ccgccgccgc
      181 caccggactc gccggtcccg cgccgactgt tcaagatcct gctacgcgcg ctgtccaccg
      241 cgccggagaa gcgctggccc accctcggcg agatgctcga cgccctcgag cacgaccccc
      301 agcgcgcccg catccgcgtc gcggcgatgg tcggcctgct cggcgccgcc tcggccggca
      361 gctacgccgt cgccatcgcc cgcgcgccgg aagcggaccg ctgcgaggcc ggcgcgaagg
      421 agatggcaag cgtgtggggc gaggagcgcc gaaccgcggt cgcccgcgcc ttcgcgagca
      481 ccggcacacc cttcgccgcc gacgtgctcc agcgcgtcga ggagcgcctc gacagctaca
      541 ccaagacctg ggcctccgag caccgcctcg cctgcgagtc gaacgccgcc ggcctcgaga
      601 cgacccgcct gatggacctg cgcttcgcct gcctcggccg gcgaaaggtg cacatgcagg
      661 cgctgctcga cgtgttcacc acggccagcg gcgaggtggt cgagaacgcg gtgcaggccg
      721 tcgcggcgct gccgggcgtg caggcctgcg ccgacgccga ggtgctgctc gccgacgtcg
      781 cgccgccggg agacccggag accgcgcgca aggtcgacgc gctgcgcgag cgactggccc
      841 gcggcgccgc gctcgagggc accggcggct acgcggaggg cctgcaggtg gtacaggcgg
      901 cccgcgccga ggcgatcacc gtcggttacg cgccgctgac cgccgaggcg gcgctcgccg
      961 agggctcgct gctgacgacc gccggtcggc tcaacgaggc cgaggcggcg ctggccgagg
     1021 cggtgcgcct cgggatcgcc caggacatgc acgcggtcgc ggccgaggcg gcggcgcgac
     1081 ggatcttcgt gatcggcgtc ggtttgcgcg atcacccgac cgcgctcgcc agccagctgt
     1141 tcgccgaggc gctcgtcgag cgcgccagcg acgacggtcg gctcgaggcc ctgctgctca
     1201 acaacctcgg caccgtgttc gccctcaagg gcgacgacgc cgcggcgcgc acccacttcc
     1261 agaagacgat cgagctgctg cgcagctccg gcgacgccga cccgctgctc gcggtcgtcc
     1321 atcacaacct ggccgagatc gacctcaagc gcggcaacct cgagggcgcc gaggacaact
     1381 cgacccgcgc gcacgcgctg ttcatcgcgc tgctcggaca gcgtcacccg ctggtcgctc
     1441 acccgctcgc aggcctcggc gacgtcgagc tcaagcgcgg caaccccgac aacctcgtcc
     1501 tcgccgacaa gcgctaccgc gaggcgctcg cgctgatgga ggccggtcac ggccccgagc
     1561 acccgtacct gttgcacccg ctgacggggc tcggtcgcgt cgctgcgcgc cagggtaacg
     1621 tcgagcaggc ccgccgtcgc ttcgaccgcg tcgtcgcgat cgccgagcgc aacgagttca
     1681 tgcacccgct gctcggcgag gcgctcgagg agctcgggga gctgagcgcg gccgagggcg
     1741 acgggagcaa ggcgctggcc tcgttcgagc gcgcggtgca ggtatacggc gacgaccacg
     1801 agctgcgcgc cgcggcgatc cgacgggccg gcgagatggc cgcgaagctc aaggacccgg
     1861 ccccggcgat cgcgtggttc gagcgcatcg tcgccagcgc ccccgggccc gtgacggacg
     1921 ctccgatgcg cctcgccgcc gcgctcgcgc tcgcgcgcct gtacgccggc agcgaccggg
     1981 tcggcccggt ctgcgagctg ctacgcgagg ccgaggccgc gttgccggtc gtcgacgacc
     2041 gccgcgagcg cgtcgcggcg ggcctcaagt cctgcccgcc gcgttaatcc cgacgccgcc
     2101 agacctcgac cttgccgacg gtgatccggc ggtcgcgctc gtagtgcgtg aggaggaagc
     2161 gacgcagcgc gacgaacttc tcgcgtggca cgctcccgcc gagcgtgatg aagatcgggc
     2221 gcgccgcgtc gaggtcgcgc aacaggcgat ccgcgtgcgg gctgtcgggg atcagcgtca
     2281 ccggcgtgct cgggcggcgc caggtgtcgt cgtcgaagcg cgtcagcagg ccgggggtct
     2341 tgtagatcgg gctcgccgac agccggtcgc tgtacgcata cacgccccag aggtgccagc
     2401 cccacaccca gatccgcgcg ccgggctcgc tgtggcgcgc gatgtggtcg gcgagcttgc
     2461 gcgccccgtc gtcgtgcggg cgcgctcgat cgacgcgggt cgcgtgcagc atcacgccgg
     2521 cgcgggccac cagcagcgcg agcaccggcg cggcgagcca gcgcgccacc ccacgccgcc
     2581 agaagcccgc cgcgagcatc ccccatgggg cggccgccag caggcacagg ggcggcagcg
     2641 cggccaggaa gtagcccttg taaaagcgca gaccgaccgc cgcgccggcg atcgccgcga
     2701 gcgcccagat ccacagcagc cgcgtcgtcg cgctcgcggt ctcgtcgcgt ggacgggccg
     2761 ccgtggcgaa caccgcgagc gtcagcggca gcgccagaaa gaacaccgtc gcggcgagcc
     2821 cctcccacgc ggcgaggtgc agcggcatat cggcgtggtg cccgatgtac gccatccccc
     2881 agtcggcccc gacgtagctc gccagcaggg cgccgagctg cccgcgtgcg aggtaatgca
     2941 gcccgatcgg cgcgtgcgcg gcgacaaagc cgagcaccac cgtcgcgccg gcgcgcagct
     3001 cccacttgcc cgcggaccgc agcgcgcacg cgatcaccgg gacgatcacg atcccggccg
     3061 gctgcttgca cagcgtcgcc acgccgcaca gcgcgccgct cgccagccac atgaccgaag
     3121 ggacaggtct gcgtcgcgca gcgaacgcga gcgcgagcgc ggcgatctgc gggagcaggg
     3181 cccaggtgac gtagttggcg tccatgctgt cgagctgcgc gtcgtgcagc gcgaacacgg
     3241 ccccgcacgc gacggccgcg ccgcgccccc acagccgcca cgcgagcgcc atgacgagca
     3301 ccagagaccc gagcgcgacg aggttcgcgg cgacctgcag cgccgcgatg tcgttgccgt
     3361 cggcgacgag cgccgcgaca taaaagcttc cgggcgcctt ccactcgacg ctgtcgacgt
     3421 agggcaggcc gccgctgcgc agcagcatcg cattgtataa aatgccgccg acgtcgtgcg
     3481 aggcgaggcc cccctgcgtg aacccgggcc agcggatcgc cgccgaggcc gcgacgatca
     3541 ccagcgcggc g
//
LOCUS       DASWNJ010001054         1514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_89455, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001054 DASWNJ010000000
VERSION     DASWNJ010001054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89455"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1514
                     /locus_tag="VGB85_17780"
     CDS             <1..>1514
                     /locus_tag="VGB85_17780"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0135943.1"
                     /translation="GRKIPTWLRKVLLRGLKVVPEERFPNIDALLAALAADPGRVRKR
                     WLAGAGLVAVAVIAGVTLAERRSSEAEVCRGAERLLVGVWDEQRAAAVQTALTATGLP
                     YARDTWPRVQEQLDLYSRSWQALHVEVCEATNVHRNQSAARMDLRMTCLAESLAELRA
                     VVGVLAEADAQVAERAVQVATGLRPLSRCADATTLTAGATLSPATATAVRDLDTLLVQ
                     VRAEHDAGRYARGLQLAREAVVAADRSGHDPARAEALYHRGMLEEGAGDFAAAERSYE
                     AAYWLGEAVAEDRVRAETAVALTELVGERLGRDHDSVQWGRHAEALLHRLGDPTDLEA
                     LLHAALGVVHNRYGRYDDALLALDRAIALTEPGDPRLTTFYRQLGNVHYRRGHYPDAE
                     AAYDRAVELGESALGPDHPDLARSISNLGESHRIQGHVDEAERCFRRSIAIWEQAFGP
                     EHSLLAPPYNGLGTLALLRGDTRAAVAHFERVRSLLSGSYGPDHPDVGAVTSNL"
BASE COUNT          193 a          651 c          492 g          178 t
ORIGIN      
        1 cgggcgcaag atcccgacct ggctgcgcaa ggtgctgctg cgcggcctca aggtcgtgcc
       61 cgaggagcgc ttccccaaca tcgacgccct gctcgcggcc ctcgccgccg accccggtcg
      121 cgtccgcaag cgctggctcg ccggcgccgg gctcgtcgcc gtcgccgtga tcgccggcgt
      181 cacgctcgcc gagcgccgca gcagcgaggc cgaggtctgc cgcggcgccg aacgcctgct
      241 cgtcggcgtg tgggacgagc agcgcgccgc cgccgtccag accgccctca ccgccaccgg
      301 cctgccctac gcccgtgaca cctggccccg cgtacaggaa caactggacc tgtactcccg
      361 gtcatggcag gccctgcacg tcgaggtctg cgaggcgacc aacgtccatc gcaatcaatc
      421 cgcggcgcgc atggacctgc gcatgacctg cctcgccgag agcctcgccg agctgcgcgc
      481 cgtcgtcggc gtgctcgccg aggccgacgc ccaggtcgcc gagcgcgccg tccaggtcgc
      541 caccggcctg cgcccgctgt cccgctgcgc cgacgcgacc acgctcaccg ccggcgccac
      601 cctctcgccc gcgaccgcca ccgccgtgcg tgacctcgac accctgctcg tccaggtccg
      661 cgccgagcac gacgccggcc gctacgcccg cggcctgcag ctcgcgcgcg aggccgtcgt
      721 cgccgccgac cgcagcggcc acgaccccgc gcgcgccgag gcgctgtatc accgcggcat
      781 gctcgaggag ggcgccggcg acttcgcggc cgccgagcgc agctacgagg ccgcgtactg
      841 gctcggcgag gccgtcgccg aggaccgcgt gcgggccgag accgcggtcg cgctgaccga
      901 gctggtcggc gagcgcctcg gtcgcgacca cgacagcgtg cagtgggggc gccacgccga
      961 ggccctgctg catcgcctcg gcgacccgac cgacctcgag gccctgctgc acgccgccct
     1021 cggcgtcgtc cacaaccgct acggccgcta cgacgacgcc ctgctcgcgc tcgatcgggc
     1081 gatcgccctc accgagcccg gtgacccgcg cctgaccacc ttctatcgcc agctcggcaa
     1141 cgtgcactac cgccgcggcc actaccccga cgccgaggcc gcctacgacc gcgccgtcga
     1201 gctcggcgag tccgccctcg ggcccgacca ccccgacctc gccagatcga tcagcaacct
     1261 cggcgagtcg caccggatcc agggccacgt cgacgaggcc gagcgctgct ttcgccggtc
     1321 gatcgcgatc tgggagcagg ccttcggccc cgagcactcg ctgctcgcgc cgccctacaa
     1381 cggcctcggc accctcgccc tgctgcgcgg cgacacgcgg gccgcggtcg cccacttcga
     1441 acgcgtgcgc tcgctgctgt ccggcagcta cggccccgac caccccgacg tcggcgccgt
     1501 caccagcaac ctcg
//
LOCUS       DASWNJ010001055         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_655310, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001055 DASWNJ010000000
VERSION     DASWNJ010001055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655310"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VGB85_17785"
     CDS             complement(<1..459)
                     /locus_tag="VGB85_17785"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0135944.1"
                     /translation="MRDGQGIDVEDVPVHSPVEDAGEASVGERLRQARTAAGLTLEQV
                     SAGTRIRVPLLRELEADRLGPASSAVYTRGHIRAIAAVVGVDPAPIVRLFDDRVGATA
                     PSLPVVAEPVPAPRRPTGSLSVPVSAPPDRTEPRWLVASLAGVTVLVALLA"
     gene            complement(568..>1509)
                     /locus_tag="VGB85_17790"
     CDS             complement(568..>1509)
                     /locus_tag="VGB85_17790"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HEY0135945.1"
                     /translation="RLVLVDPKRVELTAYEGIPHLITPIITNPKKAAEALQWVVREMD
                     MRYDDLAASGFRHVDDFNRAVRAGELVAPPGSERVYQPYPYLMVIIDELADLMMVAPR
                     DVEDSIVRITQLARAAGIHLVIATQRPSVDVVTGLIKANVPSRLAFATSSGTDSKVIL
                     DQPGAEKLIGKGDSLFLPMGASKPMRIQGALVTDAEVAAVVAHCKEQRQPAFRADVTG
                     PAPKSEKEVDEEIGDDLEVLCSAVELVVTTQFGSTSMLQRKLRVGFAKAGRLMDLMES
                     RGIVGPSEGSKARDVLVRPEELENVLVVLRGGDPVEV"
BASE COUNT          199 a          534 c          563 g          213 t
ORIGIN      
        1 ggcgagcagt gcgaccagca cggtcacgcc ggcgaggctg gcgacgagcc agcgcggctc
       61 ggtgcggtcg ggcggcgcgg agaccggcac cgacaggctg ccggtcgggc ggcgcggcgc
      121 ggggaccggc tccgcgacca ccggcagcga gggcgccgtg gccccgacgc ggtcgtcgaa
      181 caggcgcacg atgggggcgg ggtcgacgcc gacgaccgcg gcgatcgcac ggatgtgccc
      241 gcgcgtgtag acggcgctgg aggccggccc gagccggtcg gcctcgagct cgcgcagcag
      301 cgggacccgg atgcgggtgc cggcgctgac ctgctcgagc gtgagcccgg ccgccgtccg
      361 cgcctggcgc aggcgctcgc cgaccgacgc ctcgccggcg tcctcgaccg ggctgtgcac
      421 cgggacgtcc tcgacgtcga tcccctgtcc gtcgcgcacg gtcccacctc cactctcctg
      481 cagcgagccc cgatcgtagg tccgcggagc cccgcaggac gccaacgcca cgtcacgttc
      541 cagccgctga ggtgtgacgt tccgtgctca gacctccaca ggatcgccgc cgcggagcac
      601 gaccagcacg ttctcgagct cctcgggccg cacgagcacg tcacgcgcct tgctgccctc
      661 ggacggcccg acgatgccgc ggctctccat gaggtccatc agccggccgg ccttggcgaa
      721 gccgacgcgc agcttgcgct gcagcatgct cgtactgccg aactgcgtcg tgacgacgag
      781 ctcgaccgcg gagcacagca cctcgaggtc gtcgccgatc tcctcgtcga cctccttctc
      841 gctcttgggg gccgggccgg tgacgtctgc acggaacgcc ggctgccgct gctccttgca
      901 gtgcgcgacg acggcggcga cctcggcgtc ggtgacgagc gcgccctgga tgcgcatcgg
      961 cttgctcgcg cccatcggca ggaacaggct gtcgcccttg ccgatgagct tctcggcccc
     1021 cggctggtcg aggatgacct tggagtcggt gccggagctg gtggcgaacg ccaggcggct
     1081 cggcacgttg gccttgatga ggccggtgac gacgtcgacc gacggccgct gcgtcgcgat
     1141 gacgaggtgg atgccggcgg cgcgagcgag ctgggtgatg cggacgatcg agtcctcgac
     1201 gtcgcgcggc gccaccatca tgaggtcggc gagctcgtcg atgatcacca tgaggtacgg
     1261 gtagggctgg tagacccgct cgctgcccgg cggggcgacg agctcaccgg cgcggacggc
     1321 ccggttgaag tcgtcgacgt ggcggaagcc gctcgcggcg aggtcgtcgt agcgcatgtc
     1381 catctcgcgc acgacccact gcagcgcctc ggcggccttc ttggggttcg tgatgatggg
     1441 cgtgatgagg tgcgggatgc cctcgtaggc cgtgagctcg acccgcttgg ggtcgaccag
     1501 cacgaggcg
//
LOCUS       DASWNJ010001056         5101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_615853, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001056 DASWNJ010000000
VERSION     DASWNJ010001056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615853"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2058)
                     /locus_tag="VGB85_17795"
     CDS             complement(<1..2058)
                     /locus_tag="VGB85_17795"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0135946.1"
                     /translation="MESTQAASGTLPASELPRGSSADPGTIGRFVVLRRLGEGGMGVV
                     YSAFDEQLGRRVAIKLLHQADDSDLARARVYHEAQALARLSHPNVVQVYEVGEHGDGL
                     YVAMEFVTGETLRAWMNHGGHDWRDTVEVFVQAGQGLAAAHVAGLVHRDFKPENTVIG
                     EDGRARVLDFGLALHQTLPDAAPTQVVATADTHAVGVVAGTPRYMSPEQHEGRPLDAR
                     SDQFSFCVALWEALHGEPPHAGDTLLELVHNAVTGTLRPPPGRTGAPEWLRGVLIRGL
                     AVRPQDRWPDMPTLLAALRADPRVRRRRLAALALAVTTLAGLLALAMRREEPCAAVAE
                     SATEVWSPAIREAIEGAFQATGAPSAAASWTMLAGQVDAYVGEWSAAREALCVDHQAA
                     RRSDEFHDLGVECLDDRLSQLRSQLDALTGVDALAVEKATGIRSRLRPISGCADSRAL
                     RARPRPPEDPDRRTTAEALRTRIDALAARRELGQLAEAIPRAQALVAEARRIDDGPLL
                     AEALHELGRCEEALASYTSAESTLQAAFFTAGRVYHDRITAAAAWRLCFINSQIRADV
                     AASWLWLQHMEVAISRLGDPGELLAELHNARGNIHYREGRYKEALAQTERARDLLAEV
                     DPDDEMSRARYIFNIGVMHKSLGEYAAAQRAYDEALALGRRQVGEQHPFVTMLTQNMG
                     SLLL"
     gene            2436..2981
                     /locus_tag="VGB85_17800"
     CDS             2436..2981
                     /locus_tag="VGB85_17800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135947.1"
                     /translation="MTRRNARAGQQKMAYLGPKELIVMQKLRLGTFAAILLMTLSGCG
                     GGCNKDNDNDTAGPDGRPCQTIPPPAGAGYLCPEGYFCHYKEGDDMTNENLYGRCEKM
                     EGYELCMSRMLCDSPDYTPKCESILDTAYCDFYQKSHRCGDCVPPGPFVEAEDDGGIK
                     TPTTTTPTTTTPSTTTQTTTN"
     gene            2986..4047
                     /locus_tag="VGB85_17805"
     CDS             2986..4047
                     /locus_tag="VGB85_17805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135948.1"
                     /translation="MPGTFRTLFHVLGLGLCGAVIGCESDAPLPDYPLDGITVYIGFD
                     EPVCGGTFDWIEARLRWLEAETGLPAAVPPISYYWLREDVYDHCPTGACASATDNTIY
                     TPLELFSHELVHGHLAQLGLPRPWLAEGMAQLLEDSRWAVPDSPVTPTTMLETKAMDL
                     DYFSAASFVRYLRDRFGMPALIELYAALDRVDVQATPEVFLKTLGEAWDVVEDDYLAA
                     YTPTYVGSINCDFPELEPEAGTWTFPITASCEDAGSIGPFLGWIDTVAPRIERYVIVD
                     IPEAGIYSLSMTSSATVSMEIIACDEPLRSYNDYGTQIDQQIEFVPGRKRLVVSPDIA
                     DETVGGIVIRGPLSPPATP"
     gene            4136..>5101
                     /locus_tag="VGB85_17810"
     CDS             4136..>5101
                     /locus_tag="VGB85_17810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135949.1"
                     /translation="MRRLTPLLALAAACGGEPAVGFSSSSPAITSVSATSTGSESSST
                     SSTGGPESSSTSTTGSSGLVPDMGLIPDFGPVKPPGCKGKIDFLFLISRTFTMKMEQA
                     ALQTSLPGFIDTIEATFPDFDTHIMVANPDGPWPGWQCNVPELCGTKKNCGPSAPDFK
                     CGHDTWSLIEACDETLGAGIVFNAGAYATNYPCSLAEGRRYMVLPGEPDPKAAFDCIA
                     RVGTSGDYPPMGDALVAAVSPTLNAEDGCNAGFLRPDALLVVTIITDGDDTESVTKLA
                     DWYAALLNAKGDPGAVVMLAIQAQALIGETKPGCSYDDDGKLRLRD"
BASE COUNT          828 a         1777 c         1722 g          774 t
ORIGIN      
        1 cagcagcaac gagcccatgt tctgcgtcag catggtgacg aaggggtgct gctcgccgac
       61 ctggcgccgc ccgagcgcga gggcctcgtc gtaggcgcgc tgggcggccg cgtactcgcc
      121 gagcgacttg tgcatgacgc cgatgttgaa gatgtaacgg gcccggctca tctcgtcgtc
      181 ggggtcgacc tcggcgagca ggtcgcgggc ccgctcggtc tgcgcgaggg cctccttgta
      241 gcgaccctcg cggtagtgga tgttgccgcg cgcgttgtgg agctcggcga gcagctcgcc
      301 gggatctccg agccgcgaga tcgcgacctc catgtgctgc aaccacagcc acgaggccgc
      361 gacgtcggcg cggatctggc tgttgatgaa gcacaggcgc cacgcggccg cggccgtgat
      421 gcggtcgtgg tagacgcggc cggcggtgaa gaaggcagcc tgcaacgtcg actcggccga
      481 cgtgtagctg gcgagcgcct cctcgcagcg accgagctcg tggagggcct cggccagcag
      541 cgggccgtcg tcgatgcgcc gcgcctcggc gaccagcgcc tgcgcgcggg ggatggcctc
      601 cgccagctgg ccgagctcgc gacgagcggc gagcgcgtcg atgcgggtgc gcagcgcctc
      661 ggcggtggtc cgccggtcgg ggtcctcggg agggcgggga cgggcccgca gcgcccgcga
      721 gtcggcgcag ccggagatcg gccgcaggcg tgagcggatc ccggtcgcct tctcgacagc
      781 gagcgcatcg accccggtga gcgcgtccag ctgcgagcgc agctgcgaga gccggtcgtc
      841 gaggcactcg acgccgaggt catggaactc gtcggaccgg cgcgcggcct gatggtcgac
      901 gcacagcgcc tcgcgggccg ccgaccactc accgacgtac gcgtcgacct gacccgcgag
      961 catggtccag ctcgcggccg cagacggcgc gcccgtggcc tgaaaagcgc cctcgatcgc
     1021 ctcccggatc gcggggctcc agacctcggt cgcggactcg gccaccgccg cgcagggttc
     1081 ctcgcgacgc atcgccagcg cgagcagccc ggcgagcgtc gtcacggcga gcgcgagcgc
     1141 ggcgaggcgc cgccgccgaa cccggggatc ggcccgcagc gcggcgagca gcgtcggcat
     1201 gtcgggccag cggtcctgcg ggcgcaccgc gaggccgcgg atcagcacgc cgcgcagcca
     1261 ctccggcgcg ccggtacgcc cgggcggcgg gcgcagcgtg ccggtgaccg cgttgtgcac
     1321 gagctcgagc agggtgtcgc ccgcgtgcgg cggctcgccg tggagcgcct cccacagcgc
     1381 gacgcagaag ctaaactggt cgctgcgcgc gtcgagcggc cgaccctcgt gctgctcggg
     1441 cgacatgtaa cgcggcgtgc cggccacgac gccgacggcg tgggtgtccg cggtcgccac
     1501 gacctgcgtc ggggcggcgt ccgggagcgt ctggtgcagg gcgaggccga agtcgaggac
     1561 gcgcgcccgg ccgtcctcgc cgatgaccgt gttctctggc ttgaagtcgc gatgcacgag
     1621 gccggcgacg tgggcggcgg cgaggccctg accggcctgc acgaacacct cgacggtgtc
     1681 gcgccagtcg tgtccgccat ggttcatcca cgcgcgcagc gtctcgccgg tgacgaactc
     1741 catcgcgacg tacaggccgt cgccgtgctc accgacctcg tagacctgca cgacgttggg
     1801 atgagacagg cgcgcgagcg cctgggcctc gtggtacacg cgggcccggg ccaggtcgct
     1861 gtcgtcggcc tggtgcagca gcttgatcgc cacgcggcgg ccgagctgct cgtcgaacgc
     1921 cgagtagacg acgcccatgc cgccctcgcc gagccgacgc agaacgacga agcgaccgat
     1981 cgtgcccggg tcggcggacg atccgcgggg gagctcgctg gccggtaagg tgcccgaggc
     2041 ggcctgcgtg gactccatgc cggccgcacg ttcccacacg cgcggcgcgg ctgctacacg
     2101 gacacggccg agcaagcgcc ccgccgaggc caggcccggg ccccgcccgc cgcgacgaat
     2161 tcggggccgc tcacgctcgg gtcgcagagg ggcaccccgc ctcggacagc acggtgcccc
     2221 gacagccggt cgtgcagtcc ggcagccggc gagctgcgaa cccgcggcga ggcgcgacga
     2281 agcaagccga tgcgcgggac gtgaaggtcg ctctcgaaga tgtctggtat ctcgatgtgc
     2341 gcgaccgtga cgacgcgtcg tgctgtgact ggccaggaca ttgcatgcgt cgcaagcgcg
     2401 ctgggcaggg ctgatcatcc ccatcccgag tagggatgac caggcgtaat gctcgtgctg
     2461 gccagcagaa aatggcatat cttggcccca aggagctgat cgtgatgcaa aaacttcgac
     2521 tcggaacctt cgccgccatc ctcctgatga ccctctcggg gtgcggcggt gggtgcaaca
     2581 aagacaacga caatgacact gccggaccgg atggtcggcc ttgccaaaca attccgccac
     2641 ccgctggggc aggctacctc tgccccgagg gctacttctg ccattataag gagggtgacg
     2701 acatgacgaa tgagaatctt tacggccgat gcgaaaagat ggagggttac gagctatgca
     2761 tgagcaggat gctctgcgac tcccccgact acaccccgaa gtgtgaatcc atcctcgata
     2821 cggcgtactg cgacttctat cagaaaagcc accgctgtgg cgattgcgtt ccgccgggtc
     2881 cctttgtcga ggccgaggat gacggcggta taaagacgcc gaccacgacg acccccacta
     2941 cgacgacccc gtccactacg acgcagacca cgacgaacta gtgcgatgcc tggcacgttc
     3001 cgtactctct ttcacgtcct tgggctcggc ctttgcggtg cggtgatcgg ctgtgagtcc
     3061 gacgccccgc tcccggacta tccactcgac ggcatcaccg tgtacatcgg cttcgacgag
     3121 ccggtctgcg gcgggacctt cgactggatc gaggcgcgcc tgcgctggct cgaagcggag
     3181 acgggtcttc ccgccgccgt tcccccgatc tcctattact ggctgcgaga ggacgtatac
     3241 gaccattgcc cgaccggcgc gtgcgcaagc gcgaccgaca acaccatcta taccccgctc
     3301 gagctgttct cccacgagct cgtccacggg cacctggccc agctcggcct gccccggccc
     3361 tggctggccg aggggatggc gcaattgctc gaggactcgc gatgggccgt gcctgattcg
     3421 cccgtgacac caacgacgat gctagagacc aaggcaatgg atctcgatta cttctccgcg
     3481 gccagcttcg tccggtactt gcgtgatcgc ttcggcatgc ccgccctgat cgagctgtat
     3541 gcagcgctgg accgcgtcga cgtgcaagcg accccggagg tgttccttaa gaccctcggt
     3601 gaagcctggg acgtcgtcga ggacgactac ctggccgcct acaccccgac ctacgtgggt
     3661 tcgatcaact gcgatttccc cgagctcgag cccgaggcgg gcacctggac cttcccaatc
     3721 accgcatcgt gcgaggacgc ggggtcgatc ggtcccttcc tcggctggat agacacggtc
     3781 gccccgcgta tcgagcgcta cgtgatcgtg gatatccccg aggccggcat ttactcgctg
     3841 tcgatgacca gctccgccac ggtctccatg gagatcatcg cctgtgacga gccgctgcga
     3901 tcgtacaatg attatggcac gcagatcgac cagcagatcg agttcgtacc cggacgcaag
     3961 cgcctcgtgg tctcgccgga catcgccgat gagacagtcg gcgggatcgt aattcgcggg
     4021 cccctctcgc ccccggccac cccttgaggc ttcgccggac ccggcggccc aaccgccggg
     4081 ctcatcgacg ttgcatgggc tgacttcgcg cagcaaaggc gctaaaatga gcgccatgcg
     4141 ccgcctcacc cccctccttg ccctcgccgc cgcctgcggg ggcgagcccg cggtcgggtt
     4201 ctcgtccagc agccccgcga tcacatcggt gtccgcgacg agcaccggca gcgaatcgtc
     4261 ctcgacctca tcgacgggcg gcccggagag cagctcgacc agcaccaccg gcagctccgg
     4321 cctcgtgccg gacatggggc tcattccaga cttcgggcca gtgaagccgc ccggctgcaa
     4381 gggcaagatc gacttcctgt tcctgatctc gcgcacattc acgatgaaga tggagcaggc
     4441 ggcgctccag accagcctgc ccggcttcat cgacacgatc gaggcgacct tcccggactt
     4501 cgacacgcac atcatggtcg ccaacccgga cggtccctgg cccggctggc agtgcaacgt
     4561 gccggagctg tgcggcacga agaaaaactg tggcccgagc gcacccgact tcaagtgtgg
     4621 ccacgacacc tggtcgctga tcgaagcgtg cgatgagacc ctcggcgccg gcatcgtgtt
     4681 caacgcaggt gcgtatgcga ccaactatcc atgctcactc gccgaaggcc gtcgttacat
     4741 ggtgttgccc ggcgagcccg atccgaaggc cgcgttcgac tgtatcgccc gcgtggggac
     4801 atctggcgat tatccaccca tgggtgacgc gctcgtcgcc gcggtctcgc ccacgctcaa
     4861 cgccgaggat ggctgcaacg ccgggttctt gcggccggac gcgctgctcg tcgtcacgat
     4921 catcacggac ggcgacgaca ccgagtcagt gaccaaactc gcagactggt acgcggcact
     4981 cctgaatgcc aagggtgatc ctggcgccgt tgtcatgctg gcgatccagg cacaggccct
     5041 catcggcgag acaaagccgg gctgcagtta cgacgatgac ggaaagcttc ggctacgcga
     5101 c
//
LOCUS       DASWNJ010001057         1541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_158940, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001057 DASWNJ010000000
VERSION     DASWNJ010001057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158940"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..1009
                     /locus_tag="VGB85_17815"
     CDS             263..1009
                     /locus_tag="VGB85_17815"
                     /inference="COORDINATES: protein motif:HMM:NF022041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0135950.1"
                     /translation="MPLDVITLFQPPPSWGMPSMSPFCIKVETYLRMVDLPYTSRGGD
                     PRKNPKGKIPWIEDDGKQVTDSSDIVDYLKATYGDPLDRDLTPQQRAEALLARRTIEE
                     HLYWVLVHSRWAEDEGFSHVRALFLRFMPKVIGPVLLDRVIRRKVVKSLWAQGVGRHA
                     PADIYRRGAEDLTALSLLLGDRQYLLGDQPTSIDASLYGFLIALWLFPVDTPIKRHLG
                     TLGNLVAYTERMHNRYFGEHPPGRSPPKAA"
     gene            complement(1039..>1541)
                     /locus_tag="VGB85_17820"
     CDS             complement(1039..>1541)
                     /locus_tag="VGB85_17820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135951.1"
                     /translation="KITGPRDTLEKQLADAEAAQLNPKPVEPGDSGEPLDAPAPSDPA
                     PGGKPWKGLVIGGGVAIGLGLGAVVLAGVGGVRGARFDAKFDDPANMCVLDDQSPRCK
                     EFFDAGNASNAMAIAGGIAAPLLVGAGVALLVVGLKRRSAGREATSLVPTLAPGFAGF
                     TLSGRF"
BASE COUNT          230 a          535 c          563 g          213 t
ORIGIN      
        1 ggcaggtcgc gcgggtcggc gtcgacgccg agcagctccg cggacgccac cagcatcgtc
       61 tgctccttga ccgcgaaggt accgtcggcc atcgccaccg tcttgagcgc gcggaggacg
      121 gcgagcgact cggagggcga gaagttgaag aggggcgtca tgcgggggcc acgctaccaa
      181 aacatcgcgg cgaacggcac cccgcggggg ctcctcgcgg caggcgcgcg ggcccgcttg
      241 acgctggcgc taccggcgat ctatgccgct tgacgtgatc accctgttcc agccgccgcc
      301 gtcgtggggg atgcccagca tgagcccctt ctgcatcaag gtcgagacct acctgcgcat
      361 ggtcgacctc ccgtacacaa gccgcggcgg agacccgcgc aaaaatccca agggcaagat
      421 cccgtggatc gaggatgacg gcaagcaggt gacggattcg agcgacatcg tcgattatct
      481 caaggcgacc tatggtgacc cgctcgaccg cgacctgacg ccgcagcagc gggccgaggc
      541 gctgctggcc cgccgcacga tcgaggagca cctgtactgg gtgctggtgc acagccgctg
      601 ggccgaggac gagggctttt cgcacgtgcg cgcgctgttc ctgcggttca tgccgaaggt
      661 gatcggcccg gtcctgctgg accgggtgat ccgcaggaag gtcgtcaagt cgctgtgggc
      721 gcagggggtc ggtcgccacg cgccggccga catctatcgc cgcggcgccg aggacctgac
      781 cgcgctgtcg ctgctgctcg gcgaccggca gtacctgctc ggcgaccagc cgacctcgat
      841 cgacgccagc ctgtacggct ttttgatcgc gctgtggctg ttcccggtcg acacgccgat
      901 caagcgccac ctcggcacgc tcggcaacct cgtcgcgtac accgagcgca tgcacaaccg
      961 ctacttcggc gagcacccgc ccggccgcag cccgccgaag gcggcctgag gacccggcag
     1021 gcgggggcca aagcgggatc agaagcggcc ggacagggtg aagccggcga agccgggcgc
     1081 gagggtcggg accagcgagg tcgcctcgcg gccggcgctg cggcgcttga ggccgacgac
     1141 gagcagggcc acgccggcgc cgacgagcag cggcgccgcg atcccaccgg cgatcgccat
     1201 cgcgttgctg gcgttgccgg cgtcgaagaa ttctttgcag cgcgggctct ggtcgtcgag
     1261 cacgcacatg ttggcggggt cgtcgaactt ggcgtcgaag cgggcgcccc ggaccccgcc
     1321 gacgccagcg agcacgaccg cgccgaggcc gagccctatc gccacgccgc cgccgatgac
     1381 gaggcccttc cacggcttgc cgcccggggc ggggtcgctc ggcgcggggg catcgagcgg
     1441 ctcgccgctg tcgccgggct cgacgggctt ggggttgagc tgcgcggcct cggcgtcggc
     1501 gagctgcttc tcgagggtgt cgcgcggccc ggtgatcttg g
//
LOCUS       DASWNJ010001058         6092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_401671, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001058 DASWNJ010000000
VERSION     DASWNJ010001058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6092
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401671"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VGB85_17825"
     CDS             complement(<1..460)
                     /locus_tag="VGB85_17825"
                     /inference="COORDINATES: protein motif:HMM:NF015210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEY0135952.1"
                     /translation="MSGAPDDSPRALQGRPNARNFSVEDLIAAVQAGRVRIPKFQRPL
                     KWELADVLSLLDSIYRGYPVGTLLLWLRPAGEDDIYHGSVQIKAPAVADALWVVDGQQ
                     RIVSLTRALTGAGFPGEPFAAFFDLRLGTFVRPPRRDAPPSHYLPLTEVLD"
     gene            complement(519..1706)
                     /locus_tag="VGB85_17830"
     CDS             complement(519..1706)
                     /locus_tag="VGB85_17830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135953.1"
                     /translation="MSRSTRVLSILVALSVVAGAPVVQAAPAAAPAAAPAPSFATAWS
                     SSFEPTAVGSYIGDKKPNVMVVGATSASEEAAVALRAAIRTSKTAGLVIDAQAIGSTE
                     GLDDKTIVERAKGQPVGQIVIVRVFEGGPGEPPSAILTFYRPDGTVATAITSTAGTPI
                     AASGGASASAGVSAEAADAVSGIGDQAEKEAEKEATTDAKAQAQYDDQYLWFENWVGV
                     SAQTGTVVAKWSNLKQGKYGADVRGGDFYRIIGRDDLLKQYKRRRGIRAGVGTGTILL
                     GATLMTAGMVALVSNISSPGPEFGDDDPIRMGRSAGPPRISNVGASVMAGVGFSLFLG
                     GFIFVGAFRPHPTNSTEAKRLADEYNQGLRKKLGIKDRQAHLRLAPAIGRQYTGLAVS
                     GRF"
     gene            complement(1706..2395)
                     /locus_tag="VGB85_17835"
     CDS             complement(1706..2395)
                     /locus_tag="VGB85_17835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135954.1"
                     /translation="MRHIKIYTLSMGLGALSAVSLGGVSTGFVAGCARTCSDEYVECT
                     SKPGLRYVECGGARWEFNDGRGFDSEGRALDYCYCGEGMVECIDNTFVSMCNTTPLDG
                     NSIIVYDNNTSADLAPAIADCLGRPSCTLETAGCTHNGWYLDCGPGTYVTSYGEKTSQ
                     SAALESCAGGNGGHCVDAVEDCQELASCAASVACYDDYYDDGCQSFVSCYKNDDAASC
                     LADAACKWETY"
     gene            complement(2416..3258)
                     /locus_tag="VGB85_17840"
     CDS             complement(2416..3258)
                     /locus_tag="VGB85_17840"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEY0135955.1"
                     /translation="MSLLLAMMLAAMPAARADVAAPVDPLAELEMAQQRIFVRLAPSV
                     VFIHTKTGFGSGFVVSRDGLVLTNAHVVEAQATVGVVMHDGRTLTGTVLERAKSHDLA
                     LVQLPLAKTEPIPLGDSESLRVGAWAGAIGHGRGGIWTFNIGMISNIYPAESGRPVFQ
                     TQIPLNPGASGGPILDRKGQAIGIVTAKLKDAEAINFGIKIDVALRELKPLAARCECI
                     IVVAPEGVPVLVDGVMRGVGPRVVVPVSPGAHEVVTVVKDKRRRVSINFPEQRNITIG
                     LDTQ"
     gene            3405..4664
                     /locus_tag="VGB85_17845"
     CDS             3405..4664
                     /locus_tag="VGB85_17845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY0135956.1"
                     /translation="MSAQRAILHVDMDAFFASVEVRDAPELRGKPVLVGGAGKRGVVA
                     AASYEARKFGCHSAQPMGQALRLCPQAVVIPPRHDAYTEVSAQVFEIFERFSPLVEGL
                     SIDEAFIDLAGTERLWGAPRAAAEAIRAAVFAETQLTCSVGIATVKFLAKIASGMNKP
                     DGLTEIAPGTELAFITPLPVGALWGVGPRTRERLEARGIRTVGDLRALGEATLVQWFG
                     AHGQHLYRLSNAQDERAVLPDVAAKSISSEDTYATDLVGIEELRRRLLSQATRVADRL
                     VAQGIRARTVHLKIRDAAFVTETRQLTLAHPTDGYREIYGAACRLLDAVETEGRSFRL
                     TGVGVAGFEAPAAAVQLDLLAPATRGTAAPSPRSEALQAVLSAVRERHGHQALYPAEA
                     GEERRPGATGAVTQQLARPAEPRKPRR"
     gene            complement(4661..>6092)
                     /locus_tag="VGB85_17850"
     CDS             complement(4661..>6092)
                     /locus_tag="VGB85_17850"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY0135957.1"
                     /translation="RGRRRQGKRRMDHAVRRKFPGVRVRVVDRMSVIVQAAARVKHPG
                     TRVNRRYAALCGLLVCCPVLARANPPAPTPRVGPAPPPSPLAPPGDAEAMAERPFGRA
                     TYRPGGGLTIKSQDGRFALGVSLWGQLQFAAKHDQMPATGARPTTATLEFRRARLVLQ
                     GHAFSPHIKYYAHLMFSPKDLGFKDGVPTRAPVFQWYTSVTRLKNAQVQAGFFFVPYA
                     RQRMQPLPRLQFIDNSTASYEFTLDQDIGVQVSSPDIAGLGLMRYYAGVFMGEGYDWY
                     RPSDFGLTYMGRFEVLPLGMFEDFAEADFERPLKPKLSIGTAYAYSDRDRRTRAIAGT
                     GFADGGTMRSHNLTADLMFKWAGLSVLADVYLRRASRQAGDLHDTEGMPIAVQAARNG
                     HGWTAQMGLFVPRTRAEVTARSAGVRPPRTLPTSLGRLDEVGGGLNYYFYRHAFKLQL
                     DYIHTWGPALPTGRGDQLRLQLQLVF"
BASE COUNT          927 a         2178 c         2075 g          912 t
ORIGIN      
        1 agtcgagcac ctcggtgagc gggagataat gcgacggtgg cgcgtctcgg cggggcgggc
       61 gaacgaaggt cccgaggcgc aggtcgaaga acgccgcgaa cggctcccca gggaagcccg
      121 ctccggtcaa tgctcgagta agcgatacga tccgctgctg cccatcaacc acccacagcg
      181 catctgcgac tgccggggcc ttgatctgaa ccgaaccgtg gtaaatatca tcctcacccg
      241 caggtcgtag ccaaagcaac agtgtaccga ccgggtaccc ccggtaaata ctgtcgagga
      301 ggctcagcac gtccgccaac tcccatttga gcggacgctg aaatttcggg atacgaaccc
      361 gaccggcctg caccgctgcg atcagatcct cgacgctgaa attccgggcg ttgggccgcc
      421 cttgcagcgc gcgcggcgag tcgtcgggtg cgccggacat ccagcgcatc ctacaaccgc
      481 gcgaacggct tgccaacgcc gccgccgcgt ccgccgcgct agaaccggcc cgagaccgcg
      541 agtccggtgt actggcgccc gatcgcgggc gcgaggcgca ggtgggcctg gcggtccttg
      601 atgccgagct ttttgcgcag gccctggttg tactcgtcgg ccagacgctt cgcctcggtg
      661 ctgttggtcg gatgcggacg gaacgcaccg acgaagataa agccgccgag gaacaggcta
      721 aagccgacgc cggccatcac gctcgcgccg acgttggaga tccgtggtgg gcccgcactg
      781 cgacccatgc ggatcgggtc gtcgtcgccg aactcgggcc ccgggctgga aatgttgctc
      841 accagcgcga ccatgccggc ggtcatcagc gtcgcgccga gcaaaatggt gccggtgccg
      901 acgccggcgc ggatcccccg gcgacgcttg tactgcttga gcaggtcgtc gcggccgatg
      961 atgcggtaga agtcgccgcc gcggacatcc gcgccgtact tgccctgctt caggttgctc
     1021 cacttggcca cgacggtgcc ggtctgcgcg ctgacgccga cccagttctc gaaccacagg
     1081 tactggtcgt cgtactgggc ttgggccttg gcgtcggtgg tggcctcctt ctcggcctct
     1141 ttctcggcct ggtcgccgat gccggacacc gcgtcggccg cctccgcgct gacgccggcc
     1201 gaggccgacg cgccgccgct cgcggcgatc ggcgtgcccg cggtgctggt gatcgccgtc
     1261 gccaccgtgc cgtccgggcg ataaaacgtg aggatcgcgc tcgggggctc gccggggccg
     1321 ccctcgaaca cgcgcacgat cacgatctgc ccgaccggct gacccttcgc gcgctcgacg
     1381 atcgtcttgt cgtcgaggcc ctcggtcgag ccgatcgcct gcgcgtcgat gacgaggccg
     1441 gcggtcttcg acgtccggat cgcagcgcgc agcgccaccg cggcctcctc cgaggccgag
     1501 gtcgcgccga cgaccatcac gttcggcttc ttgtcgccga tgtacgagcc gaccgccgtc
     1561 ggctcgaagc tgctcgacca cgcggtcgca aaagatggcg ccggcgcggc cgcaggcgcg
     1621 gccgcaggcg ccgcctgcac gacgggcgcc ccggcgacga ccgagagcgc gacgaggatg
     1681 gagaggacac gggtgctgcg tgacatcagt aggtctccca cttgcacgcg gcgtcggcga
     1741 ggcagctggc ggcgtcgtcg ttcttgtagc aggacacgaa cgactgacag ccgtcgtcgt
     1801 aataatcgtc gtagcacgcg acgctcgccg cgcagctggc gagctcctgg caatcttcga
     1861 ccgcgtcgac gcagtggccg ccgttgccgc ccgcgcacga ctcgagcgcg gcgctctggc
     1921 tcgtcttttc tccgtaggag gtcacgtagg tcccggggcc gcagtcgaga taccagccgt
     1981 tatgcgtaca gccggccgtc tccagcgtac agctcgggcg gccgaggcag tcggcgatcg
     2041 ccggcgccag gtcggccgag gtgttgttgt cgtagacgat gatcgagttg ccgtcgagcg
     2101 gggtcgtgtt gcacatcgac acgaaggtgt tgtcgatgca ctcgaccatc ccctcgccgc
     2161 aatagcagta gtcgagcgcc cggccctcgc tgtcgaagcc gcggccgtcg ttgaactccc
     2221 agcgcgcccc gccgcattcg acgtagcgca ggccgggctt cgaggtgcat tcgacgtact
     2281 cgtcgctgca ggtacgggcg cagccggcga cgaagccggt actgacgccg ccgagcgaga
     2341 ccgccgagag ggcgccgagg cccatcgaca gggtgtagat cttgatatgg cgcacgagct
     2401 tgtttgttcc tgaggttact gcgtatcgag gccgatggtg atgttgcgct gctcggggaa
     2461 gttgatgctc acgcggcggc gcttgtcctt gacgacggtg acgacctcgt gggcgccggg
     2521 gctgaccggc acgaccacgc gaggtcccac gccgcgcatg acgccgtcga cgagcacggg
     2581 cacgccctcc ggggcgacga cgatgatgca ttcgcagcgg gcggccagcg gcttgagctc
     2641 gcgcaacgcg acgtcgatct tgatgccgaa gttgatcgcc tcggcgtcct tgagcttcgc
     2701 ggtcacgatc ccgatcgcct ggcccttgcg gtcgaggatc ggcccgcccg aggcgcccgg
     2761 gttgagcggg atctgcgtct ggaacaccgg ccgtccgctc tccgccggat agatgttgga
     2821 gatcatgccg atattgaagg tccagatgcc gccgcggccg tggccgatcg cgccggccca
     2881 cgcgcccacg cgcaggctct cgctgtcacc gagagggatc ggctcggtct tcgccagcgg
     2941 cagctggacc agcgcgaggt cgtgcgactt ggcccgctcg agcaccgtgc cggtcagcgt
     3001 gcgaccatca tgcatcacca cgccgacggt cgcctgcgcc tcgacgacgt gcgcgttggt
     3061 caacaccaga ccgtcacgcg acaccacgaa gccggatcca aagccggtct tggtgtggat
     3121 gaacaccacc gatggcgcga gccggacgaa gatccgctgc tgcgccatct ccagctcggc
     3181 gaggggatcc accggtgccg ccacgtccgc tcgcgcggcc ggcatcgccg cgagcatcat
     3241 cgccaaaagc aggctcatcc ctcgcactct accgggggcc ccgcgccccc ggcgaacaat
     3301 cttcggaccc cagcccgcac catcccgcca gccgcccgct gcgcgcgatc cctcgaagag
     3361 gcatcgcagt ggccccgtac agcggctatc ctgcgcttcc ctgaatgtct gcacagcgcg
     3421 cgattttgca cgtcgacatg gacgctttct ttgcgtcggt cgaggtgcgt gacgcccccg
     3481 agctgcgcgg caagccggtc ctcgtcggcg gcgccggcaa gcgcggggtg gtcgccgcgg
     3541 ccagctacga ggcgagaaag ttcggctgtc actcggcgca gccgatgggc caggcgctgc
     3601 gcctgtgccc gcaggcggtc gtcatcccgc cgcggcacga cgcgtacacc gaggtatcgg
     3661 cccaggtctt cgagatcttc gagcgcttct cgccgctggt ggagggcctg tcgatcgacg
     3721 aggccttcat cgacctcgcc ggcaccgagc gcctgtgggg cgcgccacgg gccgcggcgg
     3781 aggcgatccg cgcggccgtg ttcgccgaga cgcagctgac ctgctcggtc ggcatcgcca
     3841 ccgtgaagtt cctcgccaag atcgccagcg gcatgaacaa gccggacggc ctcacggaga
     3901 tcgcccccgg cacggagctc gcgttcatca caccgctgcc ggtcggcgcg ctgtggggcg
     3961 tcgggccgcg gacccgcgag cgcctcgagg cccgggggat ccgcaccgtc ggcgacctgc
     4021 gggcgctcgg cgaggccacc cttgtccagt ggttcggcgc gcacggtcaa cacttgtatc
     4081 gcctcagcaa cgcgcaggac gaacgcgccg tgctccccga cgtcgccgcc aagtcgatca
     4141 gcagcgagga cacctacgcg accgacctcg tcggtatcga ggagctgcgt cgccggctgc
     4201 tgtcccaggc gacccgcgtc gccgaccgtc tggtcgcgca gggcatccgc gcccgcaccg
     4261 tgcacctcaa gatccgcgac gccgcgttcg tcaccgagac ccgccagctg acgctcgccc
     4321 acccgaccga cggttaccgc gagatctacg gcgctgcttg ccgcctgctc gacgccgtcg
     4381 agaccgaggg tcgcagcttt cgcctcaccg gcgtcggcgt cgccggcttc gaggccccgg
     4441 ccgccgccgt ccagctcgac ctgctcgcgc cggcgacccg cggcaccgcg gcaccgtccc
     4501 cgcgcagcga ggcgctgcag gccgtgctct cggccgtgcg cgagcgtcac ggccaccagg
     4561 cgctgtaccc cgccgaggcc ggtgaggagc gccgcccggg cgcgaccggg gcggtcacgc
     4621 agcagctcgc gcgcccggcc gagccgcgca agccccggcg ctagaacacc agctgtagtt
     4681 gcaagcgcag ctgatcgccc cggccggtcg gcaacgcggg gccccaggta tggatatagt
     4741 cgagctgcag cttgaatgcg tggcgataaa agtaatagtt gaggccgccg ccgacctcgt
     4801 cgaggcgccc gaggctggtc ggcagcgtcc gtggcggccg cacgcccgcg gacctcgcgg
     4861 tgacctcggc gcgcgtgcgc ggcacgaaca ggcccatctg cgcggtccag ccgtgaccgt
     4921 tgcgcgccgc ctgcactgcg atcggcatgc cctcggtgtc gtgcaggtcg ccggcctggc
     4981 gggacgcccg gcgcagatac acgtcggcga gcaccgatag tccggcccac ttgaacatca
     5041 ggtcggcggt caggttatgc gaccgcatgg tcccgccgtc tgcaaaaccg gtccctgcga
     5101 tcgcgcgcgt gcgacggtcg cggtcgctgt acgcatacgc ggtgccgatg ctgagcttgg
     5161 gcttcaacgg gcgctcgaag tccgcctccg cgaagtcctc gaacatgccg agcggcagca
     5221 cctcgaagcg gcccatgtac gtcagaccga agtcgctcgg gcgataccag tcataaccct
     5281 cacccatgaa cacgcccgcg taatatcgca tcaggccgag cccggcgatg tcgggcgacg
     5341 acacctgcac gccgatgtcc tggtcgagcg tgaactcgta gctcgccgtc gagttgtcga
     5401 tgaactgcag gcgcggcagc ggctgcatgc gctggcgcgc gtacggcacg aagaaaaacc
     5461 ccgcctgcac ctgcgcgttc ttcagccgcg tcaccgacgt gtaccactgg aacaccggcg
     5521 cgcgcgtcgg gaccccgtcc ttgaacccga ggtccttcgg cgagaacatc aggtgcgcgt
     5581 agtacttgat gtgcggcgag aaggcgtggc cctgcaacac caggcgagcg cggcgaaact
     5641 cgagcgtcgc cgtggtcggc cgcgccccgg tggccggcat ctggtcgtgc ttcgccgcga
     5701 actgcagctg gccccacagg ctgaccccga gcgcgaagcg gccgtcctgc gactttatgg
     5761 tcaacccgcc acccggacga taggtcgcgc gcccgaacgg ccgctcggcc atcgcctccg
     5821 cgtcgccggg cggcgcgagc ggggacggtg gaggggcagg accgacgcga ggcgtcggcg
     5881 cgggcggatt ggcgcgggcg agcacggggc agcacaccag cagcccgcac agcgccgcgt
     5941 acctgcgatt tacccgcgtc cctgggtgct tcacgcgagc ggcagcttgg acgatgacgc
     6001 tcatcctgtc caccacacgg acgcgaacgc ccgggaactt tcgccgtacg gcgtggtcca
     6061 tgcgccgctt gccctgccgg cgccgacccc gg
//
LOCUS       DASWNJ010001059         1522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_331572, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001059 DASWNJ010000000
VERSION     DASWNJ010001059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331572"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /gene="preA"
                     /locus_tag="VGB85_17855"
     CDS             complement(<1..872)
                     /gene="preA"
                     /locus_tag="VGB85_17855"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006183.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HEY0135958.1"
                     /translation="MSTLIRPRLGASVVPDLTSNTGGIVTPNPFWLASAPPTNSGGQI
                     MRAFDAGWGGAVWKTLGTPIQNVSSRFGAVHYKGTRAIGFNNIELITDRPIETNLREI
                     YEVKKRYPKHGVIVSLMVETREEWQDLIKRAIDAGADGLELNFGCPHGMCERGMGSAV
                     GQEPSLNERITSWAVEYSTVPVLVKLTPNVDEIGPHGLAAQAGGAHGVSLINTIKSII
                     GVDIERKVLEPRVGSLSTNGGYCGPAVKPIALHMIASLARDPDFKLPISGIGGVSNWR
                     DAVEFILLGCSSVQV"
     gene            complement(869..>1522)
                     /locus_tag="VGB85_17860"
     CDS             complement(869..>1522)
                     /locus_tag="VGB85_17860"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0135959.1"
                     /translation="LVIAVGLGEDRMLDIPGKQLAGIHGAVAFIREFKLGQVDLSHVR
                     QAAVLGGGNTAIDAVRELVGLGVPEVTMVYRGSEAQMSGYMHEWKAAKMESVRASWRS
                     QPLEYLGAGGHVTGVRCARLGEDKRPIAGSEIEVPAQLVLLAIGQSRLGAFASALPGV
                     QVEDGRIVVDADGATGRPGVYAAGDCANGGKEVVNAAAEGKRAAQAIDRVLREGGVQ"
BASE COUNT          223 a          555 c          502 g          242 t
ORIGIN      
        1 acctggaccg acgagcagcc gagcaaaatg aactcgaccg cgtcgcgcca gttggacacc
       61 ccgccgatgc cggagatcgg cagcttgaag tcggggtcgc gcgccaggct ggcgatcatg
      121 tgcagcgcga tcggcttgac cgccgggccg cagtagccgc cgttggtcga caggctgccg
      181 acgcggggct cgagcacctt gcgctcgatg tcgacgccga tgatgctctt gatcgtgttg
      241 atcaacgaca cgccgtgcgc gccgccggcc tgcgccgcga gcccgtgcgg cccgatctcg
      301 tcgacgttcg gggtcagttt aactaacacc ggcacggtcg agtactcgac cgcccacgac
      361 gtgatccgtt cattcaagga cggctcttgc ccgaccgccg agcccatgcc gcgctcgcac
      421 atcccgtgcg ggcagccgaa gttgagctcg agcccgtccg ccccggcgtc gatcgcccgt
      481 ttaataaggt cttgccactc ctcgcgggtc tcgaccatca gcgacacgat gacgccgtgc
      541 ttcgggtagc gcttcttgac ctcgtagatc tcgcgcaggt tggtctcgat cggccgatcc
      601 gtgatcagct cgatattatt aaaaccgatc gcgcgggtgc ccttgtagtg gaccgcgccg
      661 aagcggctcg acacgttctg gatcggcgtg ccgagcgtct tccacaccgc gccgccccag
      721 ccggcgtcga aggcccgcat gatctggccg ccggaattgg tcggcggggc cgaggcgagc
      781 cagaaggggt tcggcgtgac gatgccgccg gtgttggagg tcaggtccgg gacgacgctc
      841 gcgcccagac ggggacggat cagagtactc attgcacacc cccttcacgc agcacgcggt
      901 cgatcgcctg cgcggcccgc ttgccctcgg cggccgcgtt gacgacctcc ttgccgccgt
      961 tcgcacagtc acccgcggcg tagacccccg ggcgtccggt cgcgccgtcg gcgtcgacga
     1021 cgatccgccc gtcctcgacc tgcacgccgg gcagcgccga cgcgaacgcg ccgaggcggc
     1081 tctgaccgat cgccagcagc accagctgcg ccgggacctc gatctcgctg cccgcgatcg
     1141 ggcgcttgtc ctcgccgagc cgcgcgcagc ggacccccgt cacgtggccg ccggcgccga
     1201 ggtactcgag cggctgcgag cgccagctcg cgcgcacgct ctccatcttg gcggccttcc
     1261 actcgtgcat gtagccgctc atttgcgcct cgctgccgcg gtacaccatc gtgacctcgg
     1321 ggacgccgag gccgacgagc tcgcggaccg cgtcgatcgc cgtgttgccg ccgccgagca
     1381 ccgccgcctg gcgcacgtgc gacaggtcga cctggccgag cttgaactcg cggataaagg
     1441 cgaccgcgcc gtggatcccc gcgagctgct tgccggggat gtcgagcatg cgatcttcgc
     1501 cgaggccgac cgcgatcacg ag
//
LOCUS       DASWNJ010001060         2208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_267672, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001060 DASWNJ010000000
VERSION     DASWNJ010001060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267672"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VGB85_17865"
     CDS             complement(<1..369)
                     /locus_tag="VGB85_17865"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135960.1"
                     /translation="MRAVIDIGTNSVLLLLARRGPDGALEVLRDQSTITRLGQGAGAS
                     GVLALEAIARTVDCLAEYRATAEAAGAERIVAVATEGVRMARNQSDFLEPAAEALGVP
                     VRLLSGAEEAELSYLSVARET"
     gene            complement(544..1818)
                     /locus_tag="VGB85_17870"
     CDS             complement(544..1818)
                     /locus_tag="VGB85_17870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135961.1"
                     /translation="MKRTLLGTSLLATLLLTGSFYAGQDPAIANAAAPESNVELGGTG
                     FRLLRLAVPADGRIGDILRKNATISGGFEVMDRRSIPAALIKATNFDKKGWQDIGSES
                     VILADEISGQFKFRLYDLGSGDKPVLSRGYPSTDPLKAANRFMNDVIEHYTKTPGVFG
                     SRIAFVRTNRNPTVTKNVYSVEMNGEAPSAVTNNRSLNVLPSIGPGGQVLFTSYAKRN
                     PDLWMSGGGSLSRVSTQPGLNLGGVMSPDGSTIALTLSKDGNSEIYTIDTSGGIKSRL
                     TNNAAIDGSPTWSPGGNQLAFVSSRSGGPQVFRMGSNGSGAQRVSKQGNYNQTPDWNP
                     GAGARANMIAYAGRDNSNRYDIFAVDAGSGKLTRLTQNPGRNLDPSWSPDGRMIAFQS
                     TVGGIVVANEQGNNQQQIAKAGTTPDWGPRAN"
BASE COUNT          318 a          770 c          774 g          346 t
ORIGIN      
        1 cgtctcgcgc gcgaccgaga ggtagctgag ctccgcctcc tccgccccgc tgagcagccg
       61 tacgggcacg cccagggcct ccgcggccgg ctcgaggaag tcgctctgat tcctggccat
      121 gcgcacgccc tcggtcgcca ccgcgacgat gcgctcggcc ccggccgcct ccgcggtcgc
      181 gcgatattcc gcgaggcagt cgaccgtgcg agcgatcgcc tcgagcgcca gcacccccga
      241 cgcccccgcg ccctgcccga gccgcgtgat cgtgctctgg tcgcgcagca cctcgagcgc
      301 cccgtccggc ccccgccgcg ccagcagcaa cagcacgctg ttggtcccga tgtcgatgac
      361 ggcccgcatg ccccacccta aaacgcaaca tgtcccgaag gccagcgccc cgggacatgt
      421 ctctcgcaat ttgtttcgtc cggcgaagcc cgggaggaaa ccagcactgt cacttcgggg
      481 aaagggggct ccgcccccaa tcgccgcgaa gcggcgaccc aggccccgga cggggccgtg
      541 aggtcagttc gcccgcgggc cccagtccgg cgtggtgccg gccttggcga tctgctgttg
      601 gttgttgccc tgctcgttgg cgacgacgat gccgccgacg gtcgactgga acgcgatcat
      661 gcggccatcg ggcgaccagg acgggtcgag gttacggccc gggttctggg tcaggcgggt
      721 cagcttgccg ctgccggcgt cgaccgcgaa gatgtcgtag cggttgctgt tgtcgcgccc
      781 ggcgtaggcg atcatgttgg cgcgggcgcc ggcgccgggg ttccagtcgg gcgtctggtt
      841 gtagttgccc tgcttgctga cgcgctgggc accggagccg ttggagccca tgcggaacac
      901 ctgcgggccg ccggagcggc tcgacacgaa cgcgagctgg ttgccgccgg gcgaccaggt
      961 cggcgagccg tcgatcgcgg cgttgttggt caggcgcgac ttgatgccgc cgctggtgtc
     1021 gatcgtgtag atctccgagt tgccgtcctt ggacagcgtc agggcgatcg tcgagccgtc
     1081 gggggacatc acgccgccga ggttgaggcc gggctgggtc gacacgcgcg acagcgagcc
     1141 gccgccgctc atccacaggt ccgggttacg cttggcgtag ctggtgaaca gcacctggcc
     1201 gcccgggccg atcgacggga gcacgttgag cgagcggttg ttggtgaccg cgctcggggc
     1261 ctcgccgttc atctcgaccg agtacacgtt cttggtgacg gtcgggttgc ggttggtgcg
     1321 gacgaacgcg atgcgcgagc cgaacacgcc gggcgtcttc gtgtagtgct cgatgacgtc
     1381 gttcatgaag cggttggccg ccttgagcgg gtcggtcgac gggtagccgc gcgagagcac
     1441 cggcttgtcg cccgagccga ggtcgtacag gcgaaacttg aactgcccgc tgatctcgtc
     1501 cgccaggatg acggactcgg agccgatgtc ctgccagccc ttcttgtcga agttggtggc
     1561 cttgatcagc gcggccggga tcgagcgccg gtccatcacc tcgaagccgc ccgagatcgt
     1621 ggcgttcttg cggaggatgt caccgatgcg accgtccgcg ggcaccgcga ggcgcagcag
     1681 acggaagccg gtcccaccga gctcgacgtt gctttccggg gcggccgcgt tggcgatcgc
     1741 gggatcttga ccggcgtaga acgagccagt gaggaggagg gtggcgagaa gagatgttcc
     1801 gagcagcgtg cgcttcatgg cgacaaagac ctctgagcgt gagcgggtag aaccatacat
     1861 gtgccccgtg ggcaaaggaa ccccccccgc gccgccaaag tttgcccgct ccaggtcccc
     1921 cgcggacccc cgcgggcccg gtccgccagg aaaacatcgc tgccggcctc gtgcggccga
     1981 actgcggccc tcgccgactg gaccgcacga cccccacggc cgacggccgg gccctgcggg
     2041 gctcacgcgg ggtccggccg cgcttcggcc cggctcaccg cgacttcagc cgcgacttca
     2101 gccccggacc gcatcgcccg gaccacgagg gcccacgagc gatctccacg cgagactcac
     2161 ttgaccttgc ccttttgccc cgagccactc cacgtgaaga cgtagggg
//
LOCUS       DASWNJ010001061         1817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_267677, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001061 DASWNJ010000000
VERSION     DASWNJ010001061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267677"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1817
                     /locus_tag="VGB85_17875"
     CDS             <1..>1817
                     /locus_tag="VGB85_17875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135962.1"
                     /translation="SDELRAAVAHKALTELPAGLRYAAEQGPAEAFTPVLQRTLAAGE
                     SASVLESLYRVDSEVVRPGLLAALAVVPLLPGSFRAVRHIWKMAEVRGDAEVFGLLAH
                     RFERTPANFAGKRRRTSIDGRSMDIAKELKKPDSRLAYGSATRTYLRRRTWNSIARLA
                     TLGDAIGYVRMAVGVLLPFTDGDGGEPRNITTSVNRSSWGTHFDRFASFLAFNHILFG
                     ESKRYELKGLTLTWRCKGRYRPGDPAPEGREEAYPQLWDQVPVGLLHLLSESACLPVH
                     EFASKALRANKEFCEGLDLDAIVMLLGRPYEVTARLGYELALTRYDASRPDFDLVAAL
                     ANCPVPEARRTAREWIDANRMVFAGARDLIASIVVSPHADTRAYARTLLASVVLPAGV
                     AESLIARAVALMLAFPGDDASAARAQEGGETLLRAFPRQLRGLGLGVLRDLVRHPLAG
                     VQSFAAEVLLAHETRPEDLPEDLIAALAVESKHASVRSLGVRLFGSLPDAALLQRVNL
                     LLAFATSGLADQRNAIRPVLQRLAGQPGFGDAIVPPLLITLLSKEAHEGVHSSVLALL
                     KTDLGDALGSVHKDAVLRLLRSKYPAAQELGGILLGRNV"
BASE COUNT          241 a          628 c          676 g          272 t
ORIGIN      
        1 ggagcgacga gctgcgcgcg gcggtcgccc acaaggcgct gaccgagctg ccggcgggtc
       61 tgcgctacgc ggccgagcag gggccggcgg aggcgttcac gccggtattg cagcggacgc
      121 tcgcggcggg cgagtcggcg agcgtgctcg agtcgctgta ccgcgtcgac tccgaggtcg
      181 tgcgtccggg gttgctggcg gcgctggccg tcgtgccatt gctcccgggc agctttcgcg
      241 cggtgcggca catctggaag atggccgagg tgcgcggcga cgccgaggtg ttcggtctgc
      301 tggcgcatcg cttcgagcga acgccggcga acttcgccgg caagcgccgg cgcacgtcga
      361 tcgacgggcg ctcgatggac atcgccaagg agctcaagaa gcccgacagt cgcctggcct
      421 acggcagcgc gacgcggacc tacctgcgcc gccggacctg gaactcgatc gcgcgactgg
      481 cgacgctcgg ggacgcgatc ggctacgtgc gcatggcggt cggggtgttg ctgccgttca
      541 ccgatggcga tggtggtgag ccgcgcaaca tcaccacctc cgttaaccgc tccagctggg
      601 gaacgcactt cgatcgcttc gcgagcttcc tcgcgttcaa ccatatcctg ttcggcgaga
      661 gcaagcgcta cgagctcaag gggctgacgc tgacgtggcg ctgcaagggc cgctacaggc
      721 ccggagaccc ggcgccggag ggccgcgagg aggcataccc gcagctgtgg gaccaggtgc
      781 cggtcggcct gctgcatttg ctttccgaga gcgcgtgctt gcccgtccat gagttcgcga
      841 gcaaggccct gcgcgccaac aaggagttct gcgagggcct cgacctcgac gcgatcgtga
      901 tgctgctcgg ccggccgtac gaggtcacgg cgcggctcgg ctacgagctc gcgctaacgc
      961 gctatgacgc gagccgcccg gacttcgacc tcgtggcggc gctggcgaat tgcccggtgc
     1021 ccgaggcgcg acgcacggcg cgcgagtgga tcgacgccaa ccgcatggtg ttcgccgggg
     1081 cgcgcgacct gatcgccagc atcgtggtct cgccccacgc cgacacgcgg gcctacgcgc
     1141 gcacgttgct ggccagcgtc gtgttgccgg ccggcgtcgc cgagagcctg atcgcgcgcg
     1201 cggtggcgct gatgctcgcg ttcccgggtg acgatgcatc cgcggcgcgg gcccaggagg
     1261 gcggcgagac gctgctgcgc gcgttcccga ggcagctgcg cggcctcggc ctgggggtgc
     1321 tgcgtgacct cgtgcgccac ccgctcgcgg gcgtgcagag cttcgcggcc gaggtgctgc
     1381 tggcccacga gacccgcccg gaggacctgc ccgaggacct gatcgcggcg ctcgcggtcg
     1441 agtcgaagca cgcgagcgtg cgcagcctcg gcgtgcgcct gttcggcagc ctgccggacg
     1501 ccgcgctgct gcagcgcgtc aacctgctgc tcgccttcgc gacctcgggc ctggccgatc
     1561 agcgcaacgc gatccgcccg gtgctgcagc gcctcgccgg ccagcccggc ttcggcgacg
     1621 cgatcgtgcc gccgctgttg atcacgctgc tgagcaagga ggcgcacgag ggcgtgcaca
     1681 gctcggtgct cgcgctgctc aagaccgacc tcggcgacgc gctcgggtcg gtccacaagg
     1741 acgcggtgct gcgtctgttg cgctcgaagt acccggcggc gcaggaattg ggcggcatct
     1801 tgctcgggcg caacgtc
//
LOCUS       DASWNJ010001062         3308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_267726, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001062 DASWNJ010000000
VERSION     DASWNJ010001062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267726"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VGB85_17880"
     CDS             complement(<1..311)
                     /locus_tag="VGB85_17880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135963.1"
                     /translation="MRLPFLAACLCAAACGGGPGEGFASSMAPQITSVSGSAASSTSP
                     ASTSNEPGSSTSGASSTSGDSSTSGPSSTSAGATTETIYDLGAGSDVGPLQPPGCKDK
                     I"
     gene            402..1211
                     /locus_tag="VGB85_17885"
     CDS             402..1211
                     /locus_tag="VGB85_17885"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEY0135964.1"
                     /translation="MEADAKTDDLAKLYTFAAWMLNDRAEGLRRAAEVVRAAPGLGFV
                     QWVEAVIAPLSGATGRTGPKTERPPERKVVLMALDALLRTELTVTAGDHPEVQRDPRR
                     LRVLQWELKRTCLAAVLQGLPPSPRATFVLTKVLGFSPEQLTLMFGVTAGSVDTNLSR
                     AVKVLNEYLGARCQHLAPGNSCRCETRLGVALVHGFVRWPLNPNEAPDLPVYSQADSD
                     VGLLYASLPGFTLGDAARRVLQSAIPSGRAGGVDPKPDARAAAQLETGNRL"
     gene            1498..2196
                     /locus_tag="VGB85_17890"
     CDS             1498..2196
                     /locus_tag="VGB85_17890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0135965.1"
                     /translation="MADPEQVLIVEDDVDIAELLGELLTQEGYAVTLASNAAQGLAQL
                     RSRRFVLVLSDHRMPGQTGLAMLQEAEREGLLAGVAVLIVSAEADALRDSPWPALRKP
                     VDFDVLLDEVHRAVVARVAPEEPLVAATENASVMPVLKLVLYVTATSASSRRAQRNLE
                     RVLAQYDVAAVAVTMIDLSRDADAVDPEDRVAFTPTLVRRSPEPRAWLLGDLRDLGAV
                     RSLLEDAGVARRTS"
     gene            2201..>3308
                     /locus_tag="VGB85_17895"
     CDS             2201..>3308
                     /locus_tag="VGB85_17895"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEY0135966.1"
                     /translation="MTSEMEKITTKIPGFEVLTRGGIPRGRTTLVTGRSGTGKSIFAL
                     QVACNLAAAGEPTIYVAVEERPGDLQTTADSLGLGASALVEAKALQFLDLRRPSSGPT
                     LVTGEYDISGLYEILFARAAVIGAKLIVLDSITALFRPRPDMNTIRTLVFQLVNAIED
                     HGLTLILTAEAPHDYSRATTLGVEDYVCDLVVVLRNVIDGKRRRRSVEVQKYRRSAHY
                     KGEYPFTITERGLTIFPMDAVREVEVEAGERYSSGLAGLDAMSSGGWLRDSIVLVRGP
                     TGSGKTTLAGMYARAGAERGEKVVYYGFEETRSMLLRNFEQIGMSMQGFLADHLHLVC
                     RYPEAMSPEDLLIEMRSDLDRHQPSLIVLDSISSL"
BASE COUNT          517 a         1084 c         1190 g          517 t
ORIGIN      
        1 tcgatcttgt ccttgcagcc ggggggctga agtgggccca cgtcgctgcc cgcgccgagg
       61 tcgtagatcg tctcggtggt ggcgcccgcc gaggtcgagc tgggccccga ggtcgagctg
      121 tcccccgagg tcgagctggc ccccgaggtc gagctgccgg gctcgttcga ggtcgaggcc
      181 ggcgaggtcg agctggccgc actcccggac accgacgtga tctgcggcgc catcgacgag
      241 gcaaagccct cgccagggcc gccgccgcac gcggcggcgc aaagacaggc tgcgaggaac
      301 ggcagacgca tcgccggata gtaataccaa gtaggggcga agtcacgcct ctgcgctttc
      361 accgccgttc ccccgcgcca aagtttggta aagaaaggga aatggaagcc gacgccaaga
      421 cggatgacct ggcgaagctc tacacgttcg ccgcgtggat gctcaacgat cgggcggagg
      481 gccttcggcg cgccgcggag gtcgtgcgag ccgctcctgg tttgggcttc gtccagtggg
      541 tggaggccgt gatcgcaccg ctctctggcg ccacgggcag gacgggacca aagacggaga
      601 gacccccgga gcgtaaagtc gttctgatgg cattagatgc gctcctgcgc accgagctga
      661 cggtcacagc aggggatcac cccgaggtcc aacgtgaccc tcgccgcctg cgcgtgctgc
      721 aatgggagct aaagcgcacg tgccttgccg cggtgttgca agggctcccg ccttctcccc
      781 gcgcgacctt tgtccttaca aaagttctag ggttttcgcc agagcaactc acgttgatgt
      841 ttggtgttac agcggggtcc gtcgacacaa acctctcgcg tgccgtgaag gtgctaaacg
      901 aatacctcgg cgcacgttgc cagcatttag cgcccggcaa ttcgtgtcgg tgcgagacca
      961 ggctgggcgt cgcgctcgtg catggctttg tccgctggcc cttgaacccg aacgaagcac
     1021 cggatctgcc ggtgtactcg caggccgatt cggacgtcgg cctgctatac gcctcattgc
     1081 ctggattcac cttgggtgac gctgcaaggc gagtgcttca gtctgcgatc ccatctgggc
     1141 gcgctggcgg tgtcgacccg aaaccagatg cccgggctgc cgcgcagctc gaaaccggta
     1201 atcgactcta agagcacttc ccggacatcc gcgcgtacgt cctcggcgtc gcgggctgac
     1261 ggcggaccag cgcgccgcgg tgatcgagta cccgcggtga gccggtgcgg gcgtccgact
     1321 tcgcgggcgc cgcgcgagct gcgcaagggc cggggacggg tcgtcttcgc cgaccacaac
     1381 cgggtcgcgg cgaggtacat tctcggtcgg cgagggcgcc atgcgcccga cgttcaaccg
     1441 cgcaaacatc gccgcttgcg aggctcacgc ttcccgcagg ccacactgcc ctatcccatg
     1501 gccgaccccg agcaagtact gatcgtcgag gacgatgtcg acatcgccga gctgctcggt
     1561 gagctgctga cgcaggaggg ctacgcggtc acgctggcga gcaacgccgc gcaggggctg
     1621 gcgcagctgc gcagccgccg gttcgtgctg gtgctgtcgg atcaccggat gccgggtcag
     1681 accggtctgg cgatgttgca ggaggccgag cgcgagggcc tgctcgcggg ggtcgccgtc
     1741 ttgatcgtca gcgccgaggc cgacgcgctg cgcgactcgc cgtggccggc gctgcgcaag
     1801 ccggtcgact tcgacgtgct gctggacgag gtgcaccgcg cggtggtcgc tcgcgtggcg
     1861 ccggaggagc cgctcgtggc cgcgaccgag aacgcgagcg tgatgcctgt gctcaagctg
     1921 gtgttgtatg tgacggcgac gtccgcctct tcgcgccggg cccagcgcaa cctcgagcgg
     1981 gtgctcgcgc agtacgacgt cgcggcggtg gcggtgacga tgatcgacct gtcgcgggac
     2041 gcggacgcgg tcgaccccga ggaccgggtc gcgttcacgc cgacgctggt gcggcgctcg
     2101 ccggagccgc gcgcgtggct gctcggtgac ctgcgcgacc tgggcgcggt gcgctcgttg
     2161 ctggaagacg cgggcgtggc ccgcaggacg agctgaagtg atgacgagcg agatggagaa
     2221 gatcacgacg aagatccccg gcttcgaggt gctgacacgg ggagggatcc cgcgcgggcg
     2281 cacgacgctg gtgaccgggc gcagcgggac cggcaagagc atcttcgcgt tgcaggtcgc
     2341 gtgcaacctc gcggccgcgg gcgagccgac gatctacgtc gcggtcgagg agcgacccgg
     2401 cgacctgcag acgaccgccg acagcctcgg cctcggcgcc tcggcgctgg tcgaggcgaa
     2461 ggcgctgcag ttcctcgacc tgcgccgacc ctcgagcggc ccgaccctgg tcaccggcga
     2521 gtacgacatc tccggcctgt acgagatcct gttcgccagg gccgcggtga tcggcgccaa
     2581 gttgatcgtg ctcgactcga tcacggccct gttccgcccg cgcccggaca tgaacacgat
     2641 ccgcacgctg gtgttccagc tggtcaacgc gatcgaagat cacggcctga cgctgatcct
     2701 gaccgccgag gcgccgcacg actacagccg cgcgacgacg ctcggcgtcg aggactacgt
     2761 gtgcgacctc gtggtcgtgc tgcgcaacgt gatcgacggc aagcgccgcc ggcggtcggt
     2821 cgaggtccag aagtaccggc gcagcgcgca ctacaagggc gagtacccgt tcacgatcac
     2881 cgagcgcggc ctgacgatct tcccgatgga cgcggtgcgc gaggtcgagg tcgaggccgg
     2941 cgagcgctac tcgagcgggc tggcggggct cgacgcgatg agctcgggcg ggtggctgcg
     3001 cgactcgatc gtgctggtgc gcgggccgac cggcagcggc aagacgacgc tcgcgggcat
     3061 gtacgcgcgg gccggggccg agcgcggcga gaaggtcgtg tactacggct tcgaggagac
     3121 gcggtcgatg ttgctgcgca acttcgagca gatcggcatg tcgatgcagg gcttcctggc
     3181 ggaccacctg cacctggtgt gccgctaccc ggaggcgatg agcccggagg acctgctgat
     3241 cgagatgcgc agcgacctcg accggcacca gccgtcgttg atcgtgctcg acagcatctc
     3301 gtcgctcg
//
LOCUS       DASWNJ010001063         4701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_89613, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001063 DASWNJ010000000
VERSION     DASWNJ010001063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89613"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..514
                     /locus_tag="VGB85_17900"
     CDS             68..514
                     /locus_tag="VGB85_17900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135967.1"
                     /translation="MAPRSPRRPRSRLLHGPASAAAPLHGPAFAAALLAPSVAWACSG
                     PDAAAQIADAKQIGGLLFVLSLVIGVCAGWHALAPSVSRGLLGSMALLVIVHPGWWMS
                     ARGGDCGFTLIEASAVATALIVILAAVLSWRARGRSQIGSSSRALP"
     gene            complement(1327..3087)
                     /locus_tag="VGB85_17905"
     CDS             complement(1327..3087)
                     /locus_tag="VGB85_17905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135968.1"
                     /translation="MPIDRACATGLLCAAIVGCAAKPGPPAIVRVSAPAPVPDETPPA
                     PPVVAPAVTPPTRLALHLVAHRDDALLLHHFADQLMLVGAAAFARIEADGQITRLDEH
                     PGDDLPVSMLGGIDLVWHTWALGGRWPDEAWLVVSRFAQVAFVPTVHRYERRGWTRRP
                     AVHGPLYWYYRDIITWPEGQVLGLKVYEPDPWIVAWRYELGTGDDDPAHPDPAPLLAH
                     TRPRFELLGPRPRPAWPRIAAQLEVVTAAAAPDGALFLLAYRGKRSDGVAVVQRWDPA
                     RPAERAGVVDILPGRANVELLRVDAAGIAHVGGAQKSVTSPYLARFDGTTWTEIPAPE
                     GRRLHDLARAPDGVLWAITDQESADEYERDEPPGALARQGSPEAEWEPIELAGVAVPA
                     MPGWEYSDDVEPVLASRHDTQVAMAVDPRQVVVRADGELLIRGAIDLGGIAPHEVILS
                     SRPPPGPPLLLPGDRQQWAIRDDARPTRPLRRGTWCEGGLDPFILVRPVPRGTPDDAP
                     IAEVAALVNDPGLRPKIRGIHEFERAGRRVVGLFYALDDDDDRTALLAALARVAPGVR
                     RVFECRRPPIRRVLWRPGAP"
     gene            4268..>4701
                     /locus_tag="VGB85_17910"
     CDS             4268..>4701
                     /locus_tag="VGB85_17910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135969.1"
                     /translation="MRQPLSPRPLSRLETDLIDEETRVLASLLAEHPGARYFARLRLR
                     TAAGERDVVLGERGWSNARVAMIDWRSAPLAEVFFNSEPGDDYELEHEGRSLTGEVLR
                     RHLVHLQGSELVGLDLADCRLRRGSDGEWYADDRGSPRVAPRP"
BASE COUNT          703 a         1705 c         1605 g          688 t
ORIGIN      
        1 gacagggatg agccgcgtcg cggcgccgcc gcgccctcgg cgcacgaggg cgcctgtact
       61 ccgcgtcgtg gcgccgcgga gccctcggcg cccgaggtcg cgcctgctcc acggcccggc
      121 gtccgcggcg gccccgctcc acggcccggc gttcgcggcg gccctgctcg cgccgtccgt
      181 cgcctgggcc tgctcggggc cggacgcggc ggcgcaaatc gccgatgcga agcagatcgg
      241 cgggctgctc ttcgtgttgt cgctcgtcat cggcgtgtgc gccggctggc acgccctggc
      301 gccgagtgtt tcgcggggcc tcctcgggtc gatggccctg ctcgtgatcg ttcatccggg
      361 ctggtggatg agcgcgcgcg gcggggactg cggctttacc ctcatcgagg cctccgcggt
      421 cgccaccgcc ctgatcgtga tcctcgcggc ggtgctctcg tggcgcgcgc gaggccgcag
      481 ccagatcggg tcgtcttccc gggcgctccc gtgatgccac ccggcgagta ctggcagggt
      541 ccgatcgctc tgaaccggac cgccacgcac gtcctcggcg acctcctcga cgccgacgct
      601 cgcatgcccc cgagcgacgc cgcggccccg cacgtcctgc gtcgcgagga tatcgtccgc
      661 gcctcgacga gctgcggcgc ggcgtcctcg tcccaccgct cgcggagatt ggggtgctgc
      721 ggtgcccgct ccccgcgttc gtttgacgaa gatgagaacg atgacgacga gtctccgacc
      781 gacgaggctt gatacggacg ggagagatta cgtcatgtcg agcttgtcac cgacggcgtg
      841 ggcgcggctc gctgcgcctg ctgcctgcgc cggccatacc gggtcatgcc tcgcggcttg
      901 tacaccgcga gacccctagc gacgagcaac gccacagggg cctcaccggc gtcggctgtc
      961 cgcaaggtca tgtcacaaca gacatgcctg aagtgccaga gtgcccgacg ccatgtatgg
     1021 gcggaccgca ggcaggcggc ctcacggccg ccggctgggg ccggcgctag atggccacgg
     1081 gctccaaatg cgcgacggcg cgatccctct ctggcctgcc aaatttggct ggattttgtc
     1141 gcgattttgg ctggattcga gacaaggtca cgaattctgg cagctacaac cccgtagcac
     1201 ctcgcgttaa ccacatcacc aacgtccagc tcttcgaacg ccgcgaaggg gccgcaaggg
     1261 ggtcccaggg ccggtttata tggaaccgtt ccacatcctc gtgccacagc ctgacccgcg
     1321 gctgcatcac ggcgcgccgg gtcgccagag cacgcgccgt atcggcggcc ggcgacactc
     1381 gaacacccgc cgcacgccgg gcgcgacgcg tgcgagcgcg gcgagcagcg cggtcctgtc
     1441 gtcgtcgtcg tcgagcgcgt agaacaggcc gacgacccga cggcccgcac gctcgaactc
     1501 gtggatgccg cggatcttcg ggcgcagccc cgggtcgttc acgagcgcgg cgacctcggc
     1561 gatcggcgcg tcgtccggcg tgccccgggg caccgggcgg accaggatga aggggtcgag
     1621 gccgccctcg caccatgtcc cgcggcgaag cggacgcgtc ggccgggcgt cgtcgcggat
     1681 tgcccactgc tggcgatcgc ccggcagcag caacggcgga cccggcggcg gccgactcga
     1741 caggatgacc tcgtggggcg caataccgcc gaggtcgatc gccccgcgga tcagcagctc
     1801 gccgtcggca cggacgacga cctgacgcgg atcgacggcc atggcgacct gcgtgtcgtg
     1861 gcgactggcg agcaccggct cgacgtcgtc gctgtactcc cagcctggca tcgccggcac
     1921 ggcgacgccc gcgagttcga tgggctccca ttcggcctcg gggctgccct ggcgggcgag
     1981 cgcgccgggg ggctcgtctc tctcgtactc gtcggcgctc tcctgatcgg tgatcgccca
     2041 caggacgccg tcgggtgcgc gcgccaggtc gtggagccgt cggccttccg gcgcagggat
     2101 ctccgtccag gtcgtgccat cgaagcgcgc gagatagggc gacgtgaccg acttctgggc
     2161 gccgccgacg tgagcgatcc ccgcggcgtc gacgcgcaac agctcgacgt tcgcgcggcc
     2221 gggcaggata tcgacgacgc ccgcgcgctc ggccgggcgc gccggatccc agcgctgcac
     2281 gacggcgacg ccatcacttc gcttaccgcg atatgccagc aggaacaagg cgccgtccgg
     2341 cgcggcggcg gccgtcacga cctcgagctg cgccgcgatc cggggccagg ccggccgcgg
     2401 acgcgggccg agcagctcga agcgtggacg cgtgtgtgcc agcagcggcg cggggtcggg
     2461 gtgggccgga tcgtcgtcgc cagtcccaag ctcgtagcgc caggccacga tccagggatc
     2521 tggctcgtac accttgaggc caaggacctg cccctcgggc caggtgatga tatcccggta
     2581 gtaccagtaa agcggcccgt ggacggccgg gcggcgggtc cacccgcggc gctcgtatcg
     2641 gtgcacggtg gggacgaagg ccacctgggc gaacctgctg acgaccagcc aggcctcgtc
     2701 gggccagcga ccgccgagcg cccacgtatg ccacaccagg tcgatacccc ccagcatcga
     2761 cacgggtagg tcatcgccgg gatgctcgtc gaggcgggtg atctggccgt cggcctcgat
     2821 gcgcgcgaat gcggcggcgc cgacgagcat cagttggtcg gcgaagtggt gcagcagcag
     2881 cgcgtcgtcg cgatgtgcga cgaggtggag cgcgagccgg gtcggcggcg tgacggcggg
     2941 agcgacgact ggcggcgcgg gcggggtctc gtccggcacc ggcgccggcg ccgatacgcg
     3001 gacgatcgcc ggggggccgg gcttcgcggc gcagccgacg atcgccgcgc acagcaggcc
     3061 cgtcgcgcac gctcgatcaa tcggcaccac gcgagcatac accgatcacc gcggtcacag
     3121 aacgagcttg ctcatcagat gccagctcgc gcgcgcttcc gcgcctagtc gaagctactc
     3181 gaggagcacc gtgccgacaa tgaagccgcg acgctagctt cacctcccga gtaacagcgg
     3241 tccgatcgcg gttggtgtcg acgtgacgtc cgctcgttcc tcgacgttct ctccggtata
     3301 gatcactacg aacagggtgc ccgtgggcaa cccgcgacag cgatggatgt ttggtcgcta
     3361 ggagcactgc tcctggccgc gggagcctca acatcaaccg caggagcgac cgtcgcctcc
     3421 gggccgcgga tcgtggtcct ccacggacaa cacgcaatgt ggtcgcggcg aaccaccggc
     3481 acgccaaagc ctcggatggt ttgcgagcga ggtgcgtcaa caagatgcgc agcgacgcgc
     3541 gacctggtcc gaaggccagg cccggcaggc accgagacag cagtcaaagt cgcccagatc
     3601 cggccctggg gctgtctacc ttaccccgcc tacaagcccc cgggcgggca catgcgcgac
     3661 cgcgagatcc cttggttctg gcccgatagt gtccgatttt gtcgcgatcc tggaacggat
     3721 cgagacaact ctccgaattt gggcccgtat ggccccctac gcgaccaccc ccaccacatc
     3781 aaggttccga tttcagaggg ctcagaacaa tgtttctgcc tgcccgtcga ccggcgatct
     3841 ggaaccagga cccagcccgc ggtcaccgag atcgaacccg cggcgcgtaa gcttgtgacg
     3901 ctcgtcgttc acccagcagc ggcgccccca acccgttgac tccccagcac gacctcgcag
     3961 gtgcaggtgc tcgcccacgg ctagccgctg gctgccgccg cacgtgcggc acgccgtctg
     4021 cacgatctcc ctgcggccgc aacgccccgt tcggcggtag ctcgcacgat ctctcgagac
     4081 caatggatgg accttgcagc gttcgcttcc tgggctgcga acaccgcgat tcgaccatcc
     4141 actgtccgaa cactaacacg tccttcgcct caaccccatc cccaccgccc ccggcgcgcg
     4201 gaggaaccgc acccggccgg cgatcgctcg cgctcgcctt gaaaggtccc ggtggcacgt
     4261 tttggcgatg cgacagccgc tctcgccacg acccctttcg cgactcgaga ccgacctcat
     4321 cgacgaggag actcgcgtac tcgcaagcct gctcgccgag caccccggcg cccgctactt
     4381 cgctcgtctg cgtctacgca ccgcggccgg tgagcgcgac gtggtcctcg gcgagcgcgg
     4441 ctggagcaac gcgcgcgtgg cgatgatcga ctggcgaagc gccccgctcg ccgaggtctt
     4501 cttcaacagc gaacccgggg acgactacga gctcgaacac gagggccgct cgctcaccgg
     4561 tgaggtcctg aggcgccacc tcgtacatct gcaagggtcc gagctcgtcg gcctcgacct
     4621 cgcggactgc cgcctgcgtc ggggcagcga tggcgagtgg tacgccgatg atcgcggcag
     4681 cccgcgcgtc gccccgcgtc c
//
LOCUS       DASWNJ010001064         5783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_196761, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001064 DASWNJ010000000
VERSION     DASWNJ010001064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196761"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>5783
                     /locus_tag="VGB85_17915"
     CDS             <1..>5783
                     /locus_tag="VGB85_17915"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0135970.1"
                     /translation="VEARRRFDGDLAAETESADLSLHTADGACVLEISGMSIKRAARD
                     RFLRARPDDALLHCVLEPLDLPVATPTITLASPRLAALLGLPEITASTTPARLVIDAD
                     APLDDLISILTAALAQPTTSLTFIASGSDPISHGALRGLLRAVRHEHPDTPLRLIDTD
                     LEDPSLILRALESPEPELVLRGSQASAPRLRKPTTPAIDPPTLSSGTLLITGGTGELG
                     RVLARHLVTHHQARHLLLTSRRGPEAPDIATFTRELEALGATVDIAACDITDRDALAA
                     LLTTIPTDAPLTAVFHLAGTLDDGLFLDLTPARRARVLAPKLDGARHLDALTRDLPLT
                     AFVLFSSAAGLLGSPGQSSYAAANAALDALASERHRLGLPATSLAWGPWDALGMTAAL
                     TPGDLARMRREGLSPLSTARALALLDLALRRGEPVLALLDLDLRPRDHVPAILRGLIA
                     TNHRTTELRTRLESLPERERLPHLLLLTRTEIAATLGLTAPDQVPADRPLHELGLDSL
                     MAVDLRNRLTDRTRVTLPSTLAFDHPTAAAIAARLLASLGAPPSRPTLAPTLPTDDHA
                     IAVIGMACRFPGGVEDPAALWQLLARGEDAITDVPARRFDIDAWYDPDPAHPGTTYAR
                     TGGFVGEVDGLDAGFFRVAPIEARSLDPQGRLLLECSWEALERAGIVPAALAGTATGV
                     YVGLCGTEYVIDAMADADSIDAYSLLGTAHSAIVGRLSYWLGLEGPNFPVDTACSSSL
                     VALHLACRDLRAGDCDLALTGGANLLLSPEGFVYFSRLQALSPTGRSRAFSADADGYV
                     RAEGCGVLVLKRLADARRDGDPILALVKGSAVNQDGRSNGFTAPNGPAQERVIRRALA
                     DARLAPADIDYVECHGTGTALGDPIEVQALAAVYGEQRDPQRPVIIGSIKSNIGHTEG
                     AAGVAGVIKAILGLQHAQIPATLHVGRKNPHVPWDSLPVEVATTARPFPTHDAPHRAA
                     VSSFGFSGTNAHVILEAAEPATTSAVDTPATDRPGEHATPSLEPNTIPLLLSGRGPAL
                     AAQAARLASWLRTHPDLRLTDVAFTAAVARTHFPERAALHVATREAALEALDALAEQR
                     PHPSVHTASAREQPGVVFVFSGQGGQWPAMGRALLTQSPAFAATVTACEAAFAALTDL
                     PLRAILAGEHDVPLDRPAAIQPVLYTLALALVAAWRELGVEPAAVVGHSLGEIAAAVV
                     AGALTLEEGARVVAHRGRLLETLTDPGAIAHVELPESETRRRIATHGDALCVAGVNSP
                     GTTIVAGDPRALEALLTSLRAESIFCRPIAMGYASHSRHVDPILPAFRESLKNSLTPR
                     ATRIPLYSTITGERTPGESLGAEHWPRSLREPVRLDRALDHLCADRHAVFLEIGPHPV
                     LAMALHAAVPPHGHVAASLTREHGDRSTLIKSLADLHVHGLAVDWPTVLADTNATRVA
                     LPTYAFQRERHWLSKRARLDLGAAGLTAITHPIVRATASLADTDAHIFTGRLSLAEQP
                     WLADHVILGVTLFPATGWLELALAAAHHLGHHTLTTLELTAPLALDTTAVALQITIDP
                     PTPDGQRRLRIHTRRGDEPSICNAHGLLAPISTPRSQALPWPPPGAPVDLAALHAEIV
                     RAGVDYGPSFRGLREAWRAGSQFHARIIAPGGLASERHILHPALLDAALHVLALSRVG
                     VDAGTAMLPVAWSDVTIHSTGASELRVRVDLDDTAGSDLATLALHVTDATGAPVLDVG
                     ALQFRRASLGALQITDTLYRVDWRPLPLAAAAPSPCHILGDGPLARALGQRPLTDLAE
                     LRTLATPPTLLLVDATLAASPALALECVQLLLREPTLARCEFIWISQTTITPPIEDPA
                     SAAAWGLLRSARQEHPDRTLRAIDLDAGPIDLDLLHRALAHRDEPELALRNGAALIPR
                     LGRAPTTTPSPLSLDPDGLVWISGAGG"
BASE COUNT          740 a         2711 c         1604 g          728 t
ORIGIN      
        1 cggtcgaggc ccgccgccgc ttcgacggcg acctcgcggc cgagaccgag tccgccgacc
       61 tctcgctgca caccgccgac ggcgcctgcg tgctcgagat ctccggcatg agcatcaagc
      121 gagccgcccg cgaccgcttc ctgcgcgcgc gccccgacga cgccctgctg cactgcgtcc
      181 tcgagcccct cgacctcccc gtcgctacac cgaccatcac gctcgccagc ccacgactcg
      241 cggccctgct cggcctcccc gagatcaccg cgagcacgac cccggcccgt ctcgtcatcg
      301 acgcagatgc cccgctcgac gacctcatca gcatcctcac cgccgccctc gcgcaaccga
      361 ccaccagcct caccttcatc gcctccggat ccgatcccat ctcgcacggg gccctgcgcg
      421 gcctcctgcg cgccgtccgt cacgagcacc ccgacacccc gctgcgcctg atcgacaccg
      481 acctcgaaga tccctcgctc atcctgcgcg ccctcgagtc gcccgagccc gagctcgtgc
      541 tgcgcggcag ccaggcgagc gccccacgtc tgcgcaaacc gaccacgcca gcgatcgacc
      601 ccccgaccct ctcgtccggc accctgctga tcacaggcgg caccggcgag ctcggccgcg
      661 tgctcgcccg ccacctcgtc acccaccatc aagcccgcca tctcctcctc acttcgcgcc
      721 gcggccccga ggctccggac atcgcaacct tcacccgcga gctcgaggcc ctcggcgcca
      781 ccgtcgacat cgccgcctgt gacatcaccg accgcgacgc cctcgcagcc ctcctgacca
      841 cgatcccgac cgacgccccg ctgacggcgg tgttccacct cgcgggcacc ctcgacgacg
      901 gcctgttcct cgacctcacc cccgcccgac gcgcgcgtgt gctcgccccc aagctagacg
      961 gcgcccgaca cctcgacgcc ctcacccgcg acctcccgct gaccgccttc gtcctgttct
     1021 cctcggccgc cggcctgctc ggcagccccg gccagagcag ctacgccgcg gccaacgccg
     1081 ccctcgacgc cctcgccagc gagcgccacc gcctcggcct gcccgcgacg agcctcgcct
     1141 ggggcccgtg ggacgccctc ggcatgaccg ccgccctgac ccccggcgac ctcgcccgca
     1201 tgcgccgcga gggcctctcg cccctctcga ccgcccgcgc cctcgccctc ctcgacctcg
     1261 ccctgcgtcg cggcgagccg gtgctcgcgc tgctcgacct cgacctgcgc ccgcgcgatc
     1321 acgtccccgc gatcctgcgc ggcctgatcg ccaccaacca tcgcaccacc gagctgcgca
     1381 cccgcctcga gtccctgccc gagcgcgagc gcctgcccca cctcctcctg ctcacccgca
     1441 ccgagatcgc cgcgaccctc ggcctcacgg cccccgacca ggtccccgcc gaccgcccgc
     1501 tgcacgagct cggcctcgac tcgctgatgg ccgtcgacct gcgcaaccgc ctcaccgacc
     1561 gcacccgcgt cacgctgccc tcgaccctcg cattcgatca cccgaccgcc gccgcgatcg
     1621 cggcgcgcct gctcgcctcc ctcggcgccc caccaagtcg cccgaccctc gcccccacgc
     1681 ttcccaccga cgaccacgcg atcgccgtga tcggcatggc ctgccgcttc cccggcggcg
     1741 tcgaggatcc cgcggccctg tggcagctgc tcgcccgcgg cgaggacgcg atcaccgacg
     1801 tccccgcgcg ccgcttcgac atcgacgcct ggtacgaccc cgaccccgcg caccccggca
     1861 ccacctacgc acgcacggga ggcttcgtcg gcgaggtcga cggcctcgac gccggcttct
     1921 tccgcgtcgc accgatcgaa gccaggagcc tcgacccgca gggccgcctg ctgctcgagt
     1981 gcagctggga ggccctcgag cgcgccggca tcgtgcccgc cgcgctcgcc ggcaccgcga
     2041 ccggcgtcta cgtcggcctg tgcggcaccg agtacgtcat cgacgcgatg gccgacgccg
     2101 actcgatcga cgcctactcg ctgctcggca ccgcccacag cgcgatcgtc gggcgcctct
     2161 cctactggct cggcctcgag ggccccaact tcccggtcga caccgcctgc tcctcctcgc
     2221 tcgtcgccct ccacctcgcc tgccgtgacc tgcgcgccgg cgactgcgac ctcgcgctca
     2281 ccggcggcgc caacctgctg ctctcgcccg agggcttcgt ctacttcagc cgcctgcagg
     2341 ccctctcgcc gaccggccgc agccgcgcct tctcggccga cgccgacggc tacgtgcgcg
     2401 ccgagggctg cggcgtgctc gtgctcaagc gcctcgccga cgcccgccgc gacggcgacc
     2461 cgatcctcgc gctcgtcaag ggcagcgccg tcaaccagga cggccgcagc aacggcttca
     2521 cggcccccaa cggcccggcg caggagcgcg tgatccgtcg cgccctcgcc gacgcccgcc
     2581 tcgcgcccgc cgacatcgac tacgtcgagt gccacggcac cggcaccgcc ctcggcgacc
     2641 cgatcgaggt ccaggccctc gcggcagtct acggcgagca gcgcgacccc cagcgccccg
     2701 tcatcatcgg atcgatcaag tccaacatcg gccacaccga gggcgccgcc ggcgtcgccg
     2761 gcgtcatcaa ggcgatcctc ggcctgcaac acgcgcagat ccccgcgacc ctgcacgtcg
     2821 gccgcaagaa cccacatgtc ccatgggaca gcctcccggt cgaggtcgcg accaccgccc
     2881 gcccgttccc gacccacgac gccccccacc gcgccgccgt cagcagcttc ggcttcagcg
     2941 gcaccaacgc ccacgtcatc ctcgaggccg ccgaacccgc gacgacctcc gcagtcgaca
     3001 cgccggcgac cgacaggccg ggcgagcacg cgaccccttc cctcgagccc aacacgatcc
     3061 cgctgctgct ctccggccgc ggccccgcgc tcgcggccca ggccgcccgc ctcgcgtcct
     3121 ggctgcgcac ccaccccgac ctgcgcctca ccgacgtcgc cttcaccgca gccgtcgcgc
     3181 gcacccactt ccccgagcgc gccgccctgc acgtcgcgac ccgcgaggcc gccctcgaag
     3241 ccctcgacgc cctcgccgaa cagcgcccac acccatccgt ccacaccgca agcgcgcgcg
     3301 agcaacccgg cgtcgtgttc gtcttctccg gtcagggcgg ccagtggccc gcgatgggcc
     3361 gcgccctgct gactcaatct cccgccttcg ccgccaccgt caccgcctgc gaggccgcct
     3421 tcgccgcgct caccgacctc ccgctgcgcg ccatcctggc tggcgagcac gacgtcccgc
     3481 tcgaccgccc cgccgcgatc cagccggtcc tctacaccct cgcgctcgcg ctcgtcgccg
     3541 cctggcgcga gctcggcgtc gagccggccg cggtcgtcgg ccacagcctc ggcgagatcg
     3601 cggccgccgt cgtcgccggc gcgctgaccc tcgaggaagg cgcccgcgtc gtcgcccatc
     3661 gcggccgcct gctcgagacc ctcaccgacc ccggcgcgat cgcccacgtc gagctgccgg
     3721 agtccgagac ccggcgccgg atcgccacgc atggcgacgc gctgtgcgtc gccggcgtca
     3781 acagccccgg caccacgatc gtcgccggtg acccgcgcgc cctcgaagcc ctgctcacgt
     3841 cgttacgcgc cgagtcgatc ttctgccgcc cgatcgccat gggctacgcc tcgcactcgc
     3901 gccacgtcga tccgatcctg cccgccttcc gcgaatcttt aaaaaattcc ctcaccccgc
     3961 gcgcgacccg gatcccgctg tactcgacga tcaccggcga gcgcaccccc ggagagtccc
     4021 tcggcgccga gcactggccc cgcagcctgc gcgagccggt ccgcctcgac cgcgccctcg
     4081 atcacctgtg cgccgatcgt cacgccgtct tcctcgagat cggtccacac cccgtgctcg
     4141 ccatggccct gcacgctgcc gtcccgccgc acggccacgt cgctgcctcg ctgacccgcg
     4201 agcacggcga ccgcagcaca ctcatcaaga gcctcgcaga cttgcacgtg cacggcctcg
     4261 cggtcgactg gccgacggtc ctcgccgaca ccaacgcgac ccgcgtcgcc ctgccgacct
     4321 acgccttcca gcgcgagcgt cactggttat caaagcgcgc ccgcctcgac ctcggcgccg
     4381 ccggcctcac cgcgatcacc cacccgatcg tccgcgccac cgcctccctc gccgacaccg
     4441 acgcgcacat cttcaccggc cgtctctcgc tcgccgagca gccgtggctc gccgaccacg
     4501 tcatcctcgg cgtcaccctg ttccccgcga ccggctggct cgagctcgcc ctcgcggcgg
     4561 cccaccacct cggccaccac accctcacga ccctcgagct caccgccccg ctcgccctcg
     4621 acaccaccgc cgtcgcgctc cagatcacga tcgacccgcc gacccccgac ggccagcgca
     4681 ggctccggat ccacacccgc cgcggtgacg agccgtcgat ctgcaacgcc cacggcctgc
     4741 tcgcgccgat ctcgaccccg cgctcgcaag ctctgccctg gcccccgccc ggcgcccccg
     4801 tcgacctcgc ggccctgcac gccgagatcg tccgcgccgg cgtcgactat ggcccgagct
     4861 tccgcggcct gcgcgaagcc tggcgcgccg gctcgcagtt ccacgcccgc atcatcgccc
     4921 cgggcggcct cgccagcgag cgtcacatcc ttcaccccgc gctgctcgac gccgccctgc
     4981 acgtgctcgc cctgagccgc gtcggcgtcg acgccggcac cgcgatgctc ccggtcgcct
     5041 ggtccgacgt cacgatccac agcaccggcg cgagcgagct gcgcgtgcgc gtcgacctcg
     5101 acgacaccgc gggctccgat ctcgcgacgc tcgcgctgca cgtcaccgac gccaccggcg
     5161 cacccgtgct cgacgtcggc gccctgcaat tccgccgcgc ctccctcggc gccctgcaaa
     5221 tcaccgacac cctgtaccgc gtcgactggc gaccgctccc cctcgccgcc gccgcgccgt
     5281 ccccatgcca catcctcggt gacggcccgc tcgcccgcgc cctcggccag cgcccgctca
     5341 ccgacctcgc cgagctgcgc accctcgcca cgcccccgac cctcctgctc gtcgacgcca
     5401 ccctcgccgc cagcccggcg ctcgccctcg agtgcgtgca gctcctcctg cgcgagccca
     5461 ccctggcccg ctgcgaattc atctggatct cgcagaccac gatcacccca ccgatcgaag
     5521 accccgcctc cgcggccgcc tggggcctgc tacgcagcgc ccgacaggag caccccgacc
     5581 gcacgctacg cgcgatcgac ctcgacgccg gcccgatcga cctcgacctc ctgcaccgcg
     5641 ccctcgccca ccgcgacgag cccgagctcg ccctgcgcaa cggcgccgcc ctgatccccc
     5701 gcctcgggcg cgccccgacc accacgccgt cccccctgtc cctcgacccc gacggcctcg
     5761 tatggatctc cggcgccggc ggt
//
LOCUS       DASWNJ010001065         4107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_159012, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001065 DASWNJ010000000
VERSION     DASWNJ010001065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159012"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VGB85_17920"
     CDS             <1..236
                     /locus_tag="VGB85_17920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135971.1"
                     /translation="SKSKLRTMLNDLEKRSWIEKGVEAGTEYYLARDPLDALRRNVED
                     GHLPASIFDTVNELLEQMKRDPVVVPVVKGGLR"
     gene            233..532
                     /locus_tag="VGB85_17925"
     CDS             233..532
                     /locus_tag="VGB85_17925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135972.1"
                     /translation="MSWQVIAATTVAAIIAMSMLGPWPFVALCVALAFLAAFTQGGKL
                     RASFSFWGVKAELEGEKPISPTTIIGPAALPKSLRKATGDMGSGLAEVAAQIKRP"
     gene            complement(519..3038)
                     /locus_tag="VGB85_17930"
     CDS             complement(519..3038)
                     /locus_tag="VGB85_17930"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY0135973.1"
                     /translation="MSTITANAVPTVAAPEEAQCELPVQGMTCASCVLRVERALKATP
                     GVQDAHVNFVTGRASVSYDPAAATPDALARAIEAAGYEVPRAQESVTAELPIVGMTCA
                     SCVRRIETALRAVDGVTEATVNLVTARATVRYEPARTNPGALADAIRGAGYEVPQASA
                     PVSPRVDAGARAEAQAQAEAHEQRTMRRDFIVAAALSVPLLVVAMSHGAIPGTDGMLG
                     RWLQFALATPVVFGPGRRFLRLAWVALRHRAADMNTLVSLGVLAAWGYSTVALVAPGL
                     FPHAEHGTLPHLYFEAAAAILSFVLLGKLMETRARGRLSDAVRGLVALAPKTARRIRG
                     ELEEDVPVEALIPGDLVLVRPGERIASDGEVVRGTSAVDESMLTGESLPVDKQRGSVV
                     YGGTQNHSGALTFRITRTGGDTALARIVEAVEQAQGSRAPIARLADVVSSWFVPIVLG
                     LALVTLLVWALVDPSAAGFAVAVERFVAVLVIACPCALGLATPAAVAVGTGRGAELGI
                     LVKGGAALEAASRIDTVLLDKTGTLTTGKPELTDVVALTGAEDDLLRLVAAVERESEH
                     PVAQAIVRGAVARGVALVPASDFRTEPGLGVEADVGGRTVRVGTTAWLKRAGIDTAAL
                     EATAEELAAGGRTPSFVAVDGALAGLIAVADRPTDGARETVATLRSLGIEVAMVTGDR
                     LGTARAVAAELGIERVVAEVRPEDKARVVAEERARGRIVAMVGDGINDAPALAAAHVG
                     IAIGSGTDIAIAAADVALLRGGIAGLPTALRLARRTLTTIRQNLFWAFVYNVLGIPIA
                     AGVLAPWTGWTLSPVLASAAMSLSSVSVLMNSLRLHRFKAA"
     gene            3247..3423
                     /locus_tag="VGB85_17935"
     CDS             3247..3423
                     /locus_tag="VGB85_17935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135974.1"
                     /translation="MADPLPDQGEDASTPRWVKVFGIVFGILVVLFVVKHLAGGGFRG
                     HMPSGAAESGAHQP"
     gene            3420..3989
                     /locus_tag="VGB85_17940"
     CDS             3420..3989
                     /locus_tag="VGB85_17940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135975.1"
                     /translation="MTMPPGLRKLALTVHVTVSVGWFGAVAGFLALAVAGLNSQDAHT
                     VRGSYMAMDLITWFVIVPFCLASLLTGIVQSLGTPWGLFRHWWVVVKLLLTVLATIIL
                     LLHTQPISYLASVAAETTLASVDLRDLRIQLIADAGAALVVLLVATVLAVYKPRGTTR
                     YGRRRQRASFTAKPYAGAVTHCSMHRSAR"
BASE COUNT          599 a         1427 c         1478 g          603 t
ORIGIN      
        1 agtccaagtc caagctccgg acgatgctca acgatctgga gaagcggtcg tggatcgaga
       61 agggggtgga ggcggggacc gaatattacc tggcccgcga cccgctggac gcgctccgcc
      121 gcaacgttga ggatgggcac ctgccggcgt cgattttcga caccgttaac gagttgctcg
      181 agcagatgaa acgcgacccg gtcgtcgttc ccgtcgtgaa gggagggctg cgatgagctg
      241 gcaagtcatc gcggcgacga ccgtcgcggc gatcatcgcg atgtcgatgc tcggtccatg
      301 gcccttcgtt gcgctttgcg tcgcgctggc cttccttgcc gcatttacgc aaggaggcaa
      361 gctgagggcg agcttctcgt tttggggtgt gaaggctgag ctggagggag agaagcctat
      421 ctcgcccacc accatcatcg gtccggctgc gcttccgaag tcgctccgga aagcgaccgg
      481 tgacatgggc tcgggccttg ccgaggtggc ggcgcagatc aagcggcctt gaagcggtgc
      541 aggcgcagcg agttcatcag caccgagacg ctcgacagcg acatggcggc gctggccagc
      601 acaggcgaca gcgtccagcc ggtccacggg gcgagcacac cggccgcgat gggaatgccg
      661 aggacgttgt agacgaaggc ccagaagagg ttttgccgga tggtcgtcag cgtgcgccgg
      721 gcgagccgca gggccgtcgg caggccagcg atcccaccgc gaagcagcgc cacgtcggcg
      781 gccgcgatgg cgatgtcggt gcccgagccg atggcgatgc cgacgtgcgc cgcggcgagc
      841 gcgggggcgt cgttgatgcc gtcgccgacc atggcgacga tgcggccacg ggcgcgctcc
      901 tcggcgacga cgcgggcctt gtcctccggc cgcacctcgg cgacgacgcg ctcgatgccc
      961 agctcagccg cgaccgcgcg ggccgtaccg aggcggtcgc ccgtcaccat cgccacctcg
     1021 atgccgaggc tgcgcagggt cgcgacggtc tcgcgggccc cgtcggtcgg gcggtcggcg
     1081 acggcgatca ggccggcgag agcgccgtcc acggcgacga aggagggcgt ccgtccacct
     1141 gccgcgagct cctcggcggt agcctcgagc gccgcggtgt cgatgcccgc acgctttagc
     1201 caggcggtgg tacccacccg cacggtgcgt cccccgacat cggcctcgac gccgaggccg
     1261 ggctccgtgc ggaagtcact cgccggcacc agcgccaccc cgcgagcgac ggcgccgcga
     1321 acgatcgcct gcgcgaccgg atgctcgctc tcccgttcca cggccgccac gagccgcagc
     1381 aggtcatcct cggcgccggt gagggcgacg acgtcggtca gctccggctt gccggtggtg
     1441 agcgtgccgg tcttgtcgag caggacggtg tcgatgcggc tcgcggcctc gagggccgcg
     1501 ccacccttga cgaggatgcc gagctccgcg ccgcggccgg tgccgacagc gaccgcggcg
     1561 ggcgtggcga ggccgagggc gcacgggcag gcgatcacga gcacggcgac gaagcgctcg
     1621 acggcgacgg cgaacccggc cgcgctcgga tcgacgagcg cccacacgag cagcgtcacc
     1681 agggcgaggc cgaggacgat cgggacaaac cagctgctga cgacgtcggc caggcgggcg
     1741 atcggtgcgc gtgaaccctg cgcttgctcg acggcctcga cgatccgcgc gagcgccgtg
     1801 tcgccgccgg tgcgggtgat gcgaaaggtc aacgcgccgc tgtgattctg ggtgccgccg
     1861 taaaccaccg agccgcgttg tttgtcgacg ggcaggctct cgccggtcag catcgactcg
     1921 tcgaccgcgg acgtgccgcg cacgacctcg ccgtcgctgg cgatccgctc acccggccgg
     1981 acgagcacga ggtcaccggg gatcagcgcc tcgacgggca cgtcctcttc gagctcgcct
     2041 cggatccggc gggcggtctt cggcgcgagc gccaccaggc cacggaccgc gtcggacaac
     2101 cgccctctgg ctcgcgtctc catcagcttg ccgagcagaa cgaagctcaa gatcgccgcc
     2161 gcggcctcga aatacagatg cggcagcgtg ccgtgctccg cgtgcgggaa caggcccggc
     2221 gcgacgaggg cgaccgtcga gtagccccac gccgccagca cgccgaggct gaccagcgtg
     2281 ttcatgtcgg cggcgcggtg ccgcagcgcg acccaggcga gccgcaggaa ccggcgcccc
     2341 gggccgaaca cgaccggggt cgccagcgcg aactgcaacc accggccgag catgccgtcg
     2401 gtcccgggga tggcgccgtg cgacatggcg accaccagca gcgggacgct cagcgccgcg
     2461 gctacgatga agtctcgccg catggttcgc tgctcgtgtg cctcggcctg cgcctgcgcc
     2521 tcggcgcggg cgcctgcatc gacccgcggg gagaccgggg cagacgcctg cggcacctcg
     2581 tagcctgcgc cgcggatcgc gtcggcgagc gcgcccgggt tcgtccgcgc gggctcgtaa
     2641 cggaccgtgg cgcgcgcggt caccaggttg accgtcgcct cggtgacgcc atcgacggcc
     2701 cgcagcgccg tctcgatccg ccgcacgcac gaggcgcagg tcatgccgac gatcgggagc
     2761 tcggccgtga cggactcctg cgcgcggggg acctcgtagc ctgccgcctc gatggccctt
     2821 gccagggcgt cgggggtggc agcggcgggg tcgtacgaga cgctcgcgcg cccggtgacg
     2881 aagttgacgt gggcgtcttg gacgcccggg gtggccttga gcgcgcgctc gactcggagc
     2941 acgcagctcg cgcacgtcat gccctgcacg ggcagctcgc actgagcctc ctccggggcg
     3001 gcgacggtcg ggacagcgtt tgcggtgatg gtcgacattg ggtctcctcg gggcctgcgg
     3061 agggccctga agggctgacg cagcgggtct gtccggctta caggttcggg tgggcaggtg
     3121 tcgcgcacgt cgcgcagact cgggacatac gcgtgggccg cccctggacc gcgacactcc
     3181 accggacagc ggacgcgcat cgacatagcg atcgcggtct ggataccctg tcacgacgcg
     3241 agacccatgg ctgacccact cccggatcaa ggcgaagacg cgagcacccc gcgctgggtg
     3301 aaggtgttcg ggatcgtctt cggcatcctg gtcgtgctgt tcgtcgtcaa gcacctcgca
     3361 ggcgggggct ttcgcggtca catgccgtcc ggggccgccg agtccggcgc acaccagcca
     3421 tgacgatgcc tccgggcctg cgcaagttgg cgctcacggt gcacgtcacg gtctcggtcg
     3481 gctggttcgg tgcggtcgct ggcttcctcg cgcttgcggt cgccggtctg aacagccagg
     3541 acgcccatac ggtgcgcggc tcctacatgg cgatggacct gatcacctgg ttcgtcatcg
     3601 ttcccttttg cctggcctca ctgctgacgg gcatcgtgca gtccctgggc acgccctggg
     3661 gcttgtttcg ccactggtgg gtcgtggtga agctgctgct gaccgtcctg gcgaccatca
     3721 tcttgttgct acacacgcag ccgatcagct acttggcgag cgtcgcggca gagacgacct
     3781 tggccagcgt cgatctccgc gacttgcgta ttcagctcat cgccgacgcc ggtgccgccc
     3841 tcgtggtgct actcgtggcc accgtcctcg cggtgtacaa accgcggggc acgactcgat
     3901 acggccggcg caggcagcga gcgtcgttca ccgcgaagcc gtatgccggc gcggtcactc
     3961 attgttcgat gcacaggagc gccaggtagt tcgagcacag cagcagctcg gttgcgagcc
     4021 gcccgaggtg ccgatcgacc gtcggcttcc cctcgcctcc gatcggttgg gggcgtccaa
     4081 gccccacgct acgcaagcgg cgcccac
//
LOCUS       DASWNJ010001066         5417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_550222, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001066 DASWNJ010000000
VERSION     DASWNJ010001066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550222"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VGB85_17945"
     CDS             <1..375
                     /locus_tag="VGB85_17945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135976.1"
                     /translation="GERYMAWISTSEVQPIDRLYHARGPYKMVNGLRVAASWDALIAG
                     PLENPINVSELSETVVGPVFTGTLPNGERVMDSTHCDDWTDGGGDNFGWYGASVATNG
                     DWTAAHKSICGGHAGLYCFEQP"
     gene            792..1193
                     /locus_tag="VGB85_17950"
     CDS             792..1193
                     /locus_tag="VGB85_17950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0135977.1"
                     /translation="MARILLVEDFAEIRRLIAALLTSHGHTVVGEVDRGDEVLAAVRA
                     SEPEVVLLDISLPRRNGLDVLVDLCAAAPAVVSIMLSSHDDPYYINEARRRGAVDFVV
                     KNDAPERLIRAIADGLATQDDGAPGTGVGSP"
     gene            complement(1201..1740)
                     /locus_tag="VGB85_17955"
     CDS             complement(1201..1740)
                     /locus_tag="VGB85_17955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135978.1"
                     /translation="MLEAILHPGHVEIAAMASDPQGGLALTGVAFDALDLGPASLPAM
                     TAETPFVARLDPDAAPLWATHFQVDRLSSVAIGPTGIVAVQGNTSGTPMFGTQDPPDP
                     PPGPISSPFIVSPSFVATYEPDGAPRWYHGTQLRSGTIALDNDTVLLAGGQINDLWLS
                     GCFHYLRDREDAVVLRFHL"
     gene            complement(2007..2615)
                     /locus_tag="VGB85_17960"
     CDS             complement(2007..2615)
                     /locus_tag="VGB85_17960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135979.1"
                     /translation="MTWATLRRQRALLLGLVFGTLAAVLVARALDPAHWFRGELGRVY
                     VTGTCSYSRVAVDMLAADPGAPWVALPLAVKTPEFDGPVCHTTLARLQAAGAWWLAVI
                     PEAEACRRLQRWAGEHFAIETPAQGFPAWVGDDGAFRGFGVDPRQIAALGLTPTPKIV
                     AFWIEGGYERALVEGLGFTVPEGVEQGLPIRSPHAVPEPHRR"
     gene            complement(2612..3412)
                     /locus_tag="VGB85_17965"
     CDS             complement(2612..3412)
                     /locus_tag="VGB85_17965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135980.1"
                     /translation="MHAKQFFTVVAGVSAVCALLSLAVSGALPGFGRERASKRASPAA
                     PTYASYLQVVGVQPMDLDSARAGARRDGDAWVLGDVDLEQARSLADASQDLDVLARQD
                     EKWIPTQTWKLRHGGASPFAVAKEHCSMQHLHRMDSVCQNDLDVVVERTSDSEGRVVY
                     ARPRYEDGMDDECRAYADCVARNAWLGRESPLPAGPDELHGFKAGDIHIPVQGSKAEL
                     QAGFQDSIDVCKRNLLALSRLDQKDPIIVQNIVLNEDLLEQLEWLMHL"
     gene            complement(3439..3732)
                     /locus_tag="VGB85_17970"
     CDS             complement(3439..3732)
                     /locus_tag="VGB85_17970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135981.1"
                     /translation="MLSTTKFTLGFALSLLTATTALTQPTEPTAGWFGQCLRCEWDPW
                     GGPAYCAVSSGTGWWGCLAVGYKCTQGGNPCGKKPGGGGIAVATQSPPDLAGG"
     gene            4020..4316
                     /locus_tag="VGB85_17975"
     CDS             4020..4316
                     /locus_tag="VGB85_17975"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY0135982.1"
                     /translation="MTRRELIEYVQRKLDAGEGSTEGRLPTPQIGRVLDAAFAGIIEA
                     LRSEGRYSHPGFGTFTIKEQPARPGLHPKTGAPIEIAAATTVRFKPARELKDSL"
     gene            complement(4318..>5417)
                     /locus_tag="VGB85_17980"
     CDS             complement(4318..>5417)
                     /locus_tag="VGB85_17980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135983.1"
                     /translation="ALTGLAPAPAQQWGAIRMIPLLRSGSPGDLRLQPRKVDAGAVRL
                     NDGTAYFAWMPHAYVMRWTDDGQPIAALGAQLHGRGAPSPARHSPILHRMAKREDRNA
                     LRFVPLHLAMEGFLALHFGGPDVAWHEYSREALASGLSPRSERAMPGVWIAGLEEALR
                     VFEIHDDQVGVLVFVADALASAFVVAHPDDYRQLHRSLLGDFYGELLYQYGLLYHELQ
                     SFDLALDPATISSLSDLRAAVAVGRRQWHEHSALMSRGLLQRATRWQTIYRAGPFRLR
                     RFVGALDPDAESHIGEAIHRADGTLEYLKTYRLSAAQVKRAYLLEQLARHDWHIGRCA
                     AALGDTVHGLLVRLRNAGFGYLLRPHILRGL"
BASE COUNT          824 a         1717 c         2093 g          783 t
ORIGIN      
        1 ggcgagcgct acatggcgtg gatcagcacg agcgaggtcc agccgatcga tcgcctgtat
       61 cacgcgcgcg ggccgtacaa aatggtcaac gggctgcggg tcgcggccag ctgggacgcg
      121 ctgatcgccg gtccgctcga gaatccgatc aacgtcagcg agctcagtga gaccgtcgtc
      181 gggccggtgt tcaccggtac gctgccgaac ggcgagcgcg tgatggactc gacccactgc
      241 gacgactgga ccgacggggg cggagacaac ttcgggtggt acggggcgag cgtggcgacc
      301 aacggcgact ggacggccgc gcacaagagc atctgtggcg ggcatgcggg gctgtactgc
      361 ttcgaacagc cgtgaccgcc gcggctcatg ggccccgccg acccgatggt cgggtggtca
      421 ggccggggtg ggcgcagagt ccgacgccgg ggctgatctc ctcgcagttg gagctcgggc
      481 cgcaagcggc cacgttgacc tggcaaagga tggaggactc cggacccggg cttcgagcct
      541 gcgagcctgc agctgggtca agggctcggt ctcgtcagca tgcgcgaacg cgcgcctggc
      601 cggcggtcac gtcgagataa ccagccgggc cggcagcacg acggtgcttg cgctcgtgcc
      661 gctcgacagg tggaccggga atacgggctc gtaacagagc ggtcgtagcg cagcgtcgcg
      721 catgcagagg cctgcctcca gggtattcct gccgtgctcg aagcgcgggc ctgtgaaact
      781 atcggtcttt catggcgcgc atcctactcg tcgaggattt cgcggagatc cgcagattga
      841 tcgcggcgct gctgacgagt catggtcata cggtcgtcgg cgaggtcgac cgcggcgacg
      901 aggtcctcgc ggccgtccgt gccagcgagc ccgaggtcgt cttgctcgac atctcgcttc
      961 ctcggcgcaa cgggctcgat gtgctggtgg atctgtgcgc ggcggccccg gcggtcgtat
     1021 cgatcatgct ctcgagtcac gacgacccgt actatatcaa cgaggccagg cggcgcggag
     1081 ctgtcgactt cgtcgtcaag aatgacgcgc cggagcggct cattcgtgcg atcgccgacg
     1141 ggctcgccac gcaggacgac ggcgccccgg ggaccggcgt cggctcgccg tgagtggtgc
     1201 tcacaggtga aagcgcagca cgaccgcgtc ctcgcggtcg cggagataat gaaagcagcc
     1261 cgagagccat agatcgttga tctgcccccc ggcgagcagc acggtgtcat tgtcgagcgc
     1321 gatcgtgccg gatcgcagct gggtgccgtg ataccagcgg ggagcgccgt cgggctcgta
     1381 ggtggcgacg aacgaggggg agacgatgaa tgggctgctg atcgggccgg gcgggggatc
     1441 gggcggatcc tgcgtgccga acatcggcgt gccgctggta ttgccctgca cggcgacgat
     1501 gccggtcggg ccgatcgcga ccgacgagag gcgatcgacc tggaagtgcg tcgcccacag
     1561 cggggcggcg tccgggtcga ggcgggcgac gaagggggtc tccgcggtca tcgccggcaa
     1621 cgaggccggg ccgaggtcga gggcgtcgaa ggcgacgccg gtgagcgcca ggccgccctg
     1681 cgggtcggag gccatcgcgg cgatctcgac gtggccgggg tgcaggatcg cctcgagcac
     1741 ctcgccggtc gatgcggcgt gacgggcgag ataccagtgc cagccctcgc cgccgaccgg
     1801 ctgaccgtcg ccgatgtcga gactggagac gccgcggctg gcgaccccca gcgagccgtc
     1861 ggcggcggtg gtgaccgcgt cgacggcggc ccggccgttg gcgccatcag ggccgcgaga
     1921 gtcgcaggca gtcgccgcat cgtgagattg tatcggacct cgcggcgagg gcggcgaggg
     1981 cggcgagggc ggtgtcgggc gtcgctttag cgtcggtgcg gctcggggac cgcgtgcggg
     2041 ctgcggatcg gcaggccctg ctcgacgccc tcgggcacgg tgaagccgag gccctcgacg
     2101 agcgcgcgct cgtagccgcc ctcgatccag aaggcgacga tcttcggcgt cggtgtcagg
     2161 ccgagcgcgg cgatctgccg tgggtcgaca ccgaagccgc gaaacgcacc gtcgtcgccg
     2221 acccacgcgg ggaagccctg cgcgggggtc tcgatcgcaa aatgctcgcc cgcccaccgt
     2281 tgcaggcggc ggcaggcctc ggcctcgggg atcacggcga gccaccacgc gcccgcggcc
     2341 tgcaggcgcg cgagcgtggt gtgacacacg gggccgtcga actcgggggt tttgacggcg
     2401 agcgggaggg cgacccacgg cgcgccggga tcggcggcga gcatgtcgac ggcgacgcgg
     2461 ctgtaggagc aggtgccggt gacgtagacg cggccgagct cgccgcgaaa ccagtgcgcc
     2521 gggtcgaggg cgcgcgcgac gagcacggcg gcgagggtcc cgaagacgag cccgagcagc
     2581 agggcgcgct ggcgacgaag ggtcgcccag gtcacagatg catcagccat tcgagctgct
     2641 cgagcaggtc ctcgttgagg acgatatttt gcacaatgat cgggtctttc tggtcgaggc
     2701 ggctgagcgc gaggaggttg cgcttgcaca cgtcgatcga gtcctgaaag ccggcctgca
     2761 gctcggcctt gctgccttgc acggggatgt ggatgtcgcc ggccttgaag ccgtgcagct
     2821 cgtccgggcc ggccggcagc gggctctcgc ggccgagcca ggcgttgcgc gcgacgcaat
     2881 ccgcgtaggc gcgacactcg tcgtccatac catcctcgta gcgcgggcgg gcgtagacga
     2941 cgcggccctc gctgtcgctg gtgcgctcga ccacgacgtc gaggtcgttt tgacagacgg
     3001 agtccatgcg atgcaggtgc tgcatcgagc agtgctcctt ggcgacggcg aacggcgagg
     3061 cgccgccgtg gcgcagcttc cacgtctgcg tcgggatcca cttctcgtcc tggcgggcga
     3121 gcacgtcgag gtcctgcgag gcgtcggcga ggctgcgcgc ctgctcgagg tcgacgtcgc
     3181 cgagcaccca cgcgtcgccg tcgcggcggg cgccggcgcg ggcgctgtcg aggtccatcg
     3241 gctgcacgcc gacgacctga agataggacg cataggtggg cgccgcgggg ctcgcccgct
     3301 tgctcgcccg ctcgcggcca aagccgggca gcgcccccga caccgcgagc gagagcaggg
     3361 cacatacggc ggatacgccg gcgaccacgg tgaagaattg tttggcgtgc atggcagact
     3421 ccgggaaaat ggtggagatc agccgccggc gaggtcgggc ggggattgcg tggcgacggc
     3481 gatgcctccg ccgccaggct tctttccgca ggggttaccg ccctgcgtgc atttgtaacc
     3541 gacggcgagg caaccccacc agccggtgcc gctggaaacg gcgcaatagg ccgggccgcc
     3601 ccacgggtcc cattcgcagc gcagacactg gccgaaccat ccggcggtcg gctcggtcgg
     3661 ctgggtcagg gcggtggtgg cggtaagaag gctgagggcg aagcccaggg tgaacttggt
     3721 ggtgctcaac atgtcgtctc gttccttgag ggactgaatc ggtgttcagg tggctgcgtg
     3781 cgtccgctag agcaacggca ctgtagcgtg gtccggtcct ggcgcaagct gggcatacat
     3841 gcagtgagcg ggtccgctcc ttcattgcac ctgcatcttt tttttcagcg cgtgtttttg
     3901 cctaacgaag cgcgcatcaa cccgatcgcc tgctgcattc gctgtgttgt gcaatacagg
     3961 tttggcagcg aggcggtcgg gggatcgcgg accactgcgc atatgttagg gtcccgggca
     4021 tgacgcgacg cgagctgatc gagtacgtgc agcgcaagct ggacgcgggg gaggggtcga
     4081 cggaggggag gttgccgacg ccgcagatcg ggcgggtgct cgacgccgcg ttcgccggga
     4141 tcatcgaggc gctgcgcagc gagggccgct acagccaccc gggctttggc accttcacga
     4201 tcaaggagca gccggcgcgg ccgggcctgc accccaagac cggcgcgccg atcgagatcg
     4261 cggcggcgac gacggtgcgc ttcaagccgg cacgcgagct caaggactcg ctgtgactca
     4321 caggccgcgc aggatgtgcg gccgcagcag gtagccgaag ccggcgttgc gcaggcggac
     4381 gagcaggccg tggaccgtgt cgccgagcgc ggcggcgcag cgcccgatgt gccagtcgtg
     4441 gcgggcgagc tgctcgagca ggtaggcgcg cttcacctgc gcggcggaca ggcggtaggt
     4501 cttgaggtac tcgagggtgc cgtcggcgcg gtggatcgcc tcgccgatgt gcgactcggc
     4561 gtcggggtcg agcgcgccga cgaagcggcg caggcgaaac ggtccggcgc ggtagatcgt
     4621 ctgccagcgg gtcgcgcgct gcagcaggcc gcgcgacatc agggcgctgt gctcgtgcca
     4681 ctgccggcgc ccgaccgcga ccgcggcgcg cagatccgac agcgacgaga tcgtggcggg
     4741 gtcgagcgcg aggtcgaagc tctggagctc gtgatacagc aggccatatt gatagaggag
     4801 ctcgccgtag aagtcgccga gcaggctgcg gtgcagctgg cggtagtcgt cggggtgggc
     4861 gacgacgaag gcgctggcga gggcgtcggc gacgaacacg agcacgccga cctggtcgtc
     4921 gtggatctcg aacacgcgca gcgcctcctc gaggccggcg atccacacgc cgggcatggc
     4981 gcgctcgctg cgggggctca ggccgctggc gagggcctcg cgcgaatact cgtgccaggc
     5041 gacgtcgggc ccaccaaagt gcagcgccaa aaaaccctcc atcgccaggt gcagggggac
     5101 gaagcgcagc gcgttgcggt cctcgcgttt ggccatgcgg tggaggatcg ggctgtggcg
     5161 cgcgggggac ggggcgccgc gcccgtgcag ctgggcgccg agggcggcga tcggctggcc
     5221 gtcgtcggtc cagcgcatga cgtaggcgtg cggcatccag gcgaagtagg cggtgccgtc
     5281 gttgagccgg acggccccgg cgtcgacctt gcgcggctgc aaccgcaggt ccccggggct
     5341 gccgctgcgc agcagcggga tcatgcggat cgcgccccat tgctgggccg gcgcgggggc
     5401 caggccggtc agggcga
//
LOCUS       DASWNJ010001067         1531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_124382, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001067 DASWNJ010000000
VERSION     DASWNJ010001067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124382"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1241
                     /locus_tag="VGB85_17985"
     CDS             93..1241
                     /locus_tag="VGB85_17985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0135984.1"
                     /translation="MTMTIETRARRTAWTMMAALAVMLTGAVAVGVGPRVEQSAARDR
                     VVEEAAGPRRVRVTSVRAGDPTVDVTLPGSSAPLRSAVLYSKSTGFVRRNLVDIGDRV
                     AEGQLLAEISAPETGEEIRLARARLAEARANVGIVTTTSQRVETLARSGTSSQQEADD
                     AAYQANSAVAAVATRKADLQRLQALRDYQRIVAPFAGVVTRRLVDPGALVGPAGASGV
                     AMFEVADVDTLRVIVDVPDSHAADITPGVAVDVYSPRDPSRTVQGTVTRSSGVLEQNT
                     RTLRVEVHIPGSGAILPGSFVYVRMHVPRARPAPVIAASALVVRKEGTLVARVVDGTV
                     TLVPVKVGRDFGKEIELLEGVAPGDEVVVQPPDTLESGQAVEVVPTVS"
     gene            complement(1245..>1531)
                     /locus_tag="VGB85_17990"
     CDS             complement(1245..>1531)
                     /locus_tag="VGB85_17990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135985.1"
                     /translation="HVDAKQPLGGHAYWEVASPALADFPHQLRMMEIWDQDNGGLTIR
                     SVAFDFAADDDPVADAGRTLGVMDFTSAWEGDGRGADPADRNVELFIATP"
BASE COUNT          201 a          546 c          576 g          208 t
ORIGIN      
        1 acgctgggct tcgtgcccgt ggtctacagc ctgctcgccg cgaagtggca cccgcccgtc
       61 ctcgaccccg acctcgaacc ctccaggagc gcatgacaat gacgatcgag accagagcca
      121 ggcgcacggc gtggacgatg atggcggccc tcgcggtcat gttgaccggc gcggtcgcgg
      181 tcggcgtcgg cccacgcgtc gagcagagcg cggcgcgtga ccgggtcgtc gaggaggcgg
      241 ccgggccccg ccgcgtccgc gtgacatcgg tgcgcgccgg tgacccgacg gtcgacgtca
      301 cgctgccggg ctcgtcggcg ccgctgcgct cggcggtgct gtacagcaag tcgacgggct
      361 tcgtgcgccg caacctcgtc gacatcggcg accgcgtcgc ggagggccag ctgctcgccg
      421 agatctcggc gcccgagacc ggcgaggaga tccggctggc ccgggcgcgg ctcgccgagg
      481 cgcgcgccaa cgtcggcatc gtcacgacga cctcgcagcg cgtcgagacg ctggcccgca
      541 gcggcacgtc ctcgcagcag gaggccgacg acgcggccta tcaggccaac tcggcggtcg
      601 ctgcggtggc gacccgcaag gccgacctgc agcgcctgca ggccctgcgc gactatcaac
      661 ggatcgtggc gccgttcgcc ggcgtcgtca cgcgccggct cgtcgacccg ggcgcgctcg
      721 tcggtccggc gggcgcgagc ggggtggcga tgttcgaggt cgccgacgtc gacacgctgc
      781 gcgtgatcgt cgacgtgccg gacagccacg ccgccgacat cacgcccggc gtcgcggtcg
      841 acgtgtactc gccgagagat ccgtcgcgca cggtccaggg cacggtgacg cgctcgagcg
      901 gcgtgctcga gcagaacacc cggacgctgc gcgtcgaggt ccacatcccg ggctcgggcg
      961 cgatccttcc gggctcgttc gtgtatgtgc gcatgcacgt gccgcgggcc cgcccggcgc
     1021 cggtgatcgc cgcgagcgcc ttggtcgtac gcaaggaggg cacgctcgtc gcgcgggtcg
     1081 tcgacggcac ggtcacgctg gtgccggtca aggtcggccg cgacttcggc aaggagatcg
     1141 agctgctcga gggcgtcgcg cccggcgacg aggtcgtcgt gcagccgccc gacacgctcg
     1201 agagcggtca ggcggtcgag gtcgtgccga ccgtgtcgtg aggctcacgg cgtggcgatg
     1261 aacagctcga cgttgcggtc cgccgggtcc gcgccgcgac cgtcgccctc ccacgcgctt
     1321 gtaaaatcca tgacgccgag cgtgcggccg gcgtcggcga ccgggtcgtc gtcggcggcg
     1381 aagtcgaagg cgaccgagcg gatcgtcagg ccgccgttgt cctggtccca gatctccatc
     1441 atgcgcagct ggtgcgggaa gtcggccagg gccggcgagg cgacctccca gtaggcgtgg
     1501 ccgccgagcg gctgcttggc gtcgacgtgc a
//
LOCUS       DASWNJ010001068         2601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_689842, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001068 DASWNJ010000000
VERSION     DASWNJ010001068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689842"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VGB85_17995"
     CDS             complement(<1..186)
                     /locus_tag="VGB85_17995"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135986.1"
                     /translation="MLVSDTEIEALVVTLRASPWLAIDTEADSLHAYPEKLCLIQISH
                     AGGDVLVDPLSSASLAPL"
     gene            complement(208..642)
                     /locus_tag="VGB85_18000"
     CDS             complement(208..642)
                     /locus_tag="VGB85_18000"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEY0135987.1"
                     /translation="MTDPRSALCLACGLCCDGNLFTQVPLRADEVEPIRRRGLKIVER
                     AQGVALRQRCGALAGRRCDIYEDRPAACRSYRCMLLTALAEDEVSLGDALAIVEQAHA
                     LLAATPGEATTPVRQRLPGEATPEGARALAYLGRHFQRDAGA"
     gene            complement(639..1829)
                     /locus_tag="VGB85_18005"
     CDS             complement(639..1829)
                     /locus_tag="VGB85_18005"
                     /inference="COORDINATES: protein motif:HMM:NF015875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HEY0135988.1"
                     /translation="MARPTCPRCLRPTRVCYCEHLPSLPTRTRVIVVQHPRERGMPIG
                     TAHMARLCLPNAELHVGVRVAEMPGLQRALADTSRPIGLLYPDPGARDIIADPPPGPV
                     TLVLVDGTWAHARKLVRDNPELASLPRYAFRPPAPSEYRIRKEPSEEYVSTIESLALV
                     LGALEGDPDKFKALLIPFRAMVDAQIACAHSLGKRLTNRRPRALRILPTEVLHRREGE
                     LVCVVGEANTWPHDTPQRQVPGYRSELVHWLAIRMATGERFEAVVRPSGPLAPRTAQI
                     AGLTPEAIAAGVDGPTLGEAFDKFLHEGELVCAWGPHVLELYAGMTGAALPDTVDLRA
                     IASAMNRGGIGRLEEYANASGRVGPALGQGRGGQRLAEVVAALEHLRSLDEEDDDSDE
                     VDEQ"
     gene            1969..2175
                     /locus_tag="VGB85_18010"
     CDS             1969..2175
                     /locus_tag="VGB85_18010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135989.1"
                     /translation="MGFGKNPYVAKAQVAEQKAEEAADDNARSRHYREAAHEWDRAAE
                     REKPGKARSEYEAHALRTRELAGE"
     gene            complement(2190..>2601)
                     /locus_tag="VGB85_18015"
     CDS             complement(2190..>2601)
                     /locus_tag="VGB85_18015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135990.1"
                     /translation="PATPVAPPTVKSSAPVAKGQVEVRPWGGLAAGGGAAIGVGAAGL
                     GVMIAGAVRGKALQQRFDDPARGCDRDAPVGECGEIFRAGQAMNTQLIVGGVIGAVGL
                     VAGTTLAVLAHRRRVSAAPSVGRGFAGVVLQGRF"
BASE COUNT          395 a          957 c          915 g          334 t
ORIGIN      
        1 cagcggcgcg agcgaggccg acgacagcgg gtcgaccagc acatcaccgc cggcgtgact
       61 gatctggatc aggcacagct tctccggata cgcgtgcagg ctgtcggcct cggtgtcgat
      121 cgccagccac ggcgaggcgc gcagcgtgac gaccagcgcc tcgatctcgg tgtcgctgac
      181 gagcatgagc accgcgcgat accacggtca ggccccggcg tcgcgctgga agtgacgccc
      241 gaggtaagcc agcgcccgcg cgccctccgg cgtcgcttca cctggcaaac gctgccggac
      301 cggcgtagtg gcctctccgg gcgtagcagc gagcagtgcg tgcgcctgct ccacgatcgc
      361 cagcgcgtcg cccagcgaga cctcgtcctc cgccagcgcc gtcagcagca tgcagcggta
      421 gctgcgacac gcggccggcc gatcctcata aatgtcgcag cgacggccgg cgagcgcgcc
      481 acaacgctgg cgcagcgcca caccctgcgc gcgctcgacg atcttcaggc cgcggcggcg
      541 gatcggctcg acctcgtccg cgcgcagcgg cacctgcgtg aacaggttgc cgtcgcagca
      601 caggccgcac gcgaggcaca gcgccgagcg cgggtcggtc attgctcgtc cacctcgtcg
      661 ctgtcatcat cttcttcatc gagcgatcgc aaatgctcga gcgccgcgac gacctcggcg
      721 agccgctgac cgccgcggcc ctgcccgagc gcgggaccga cgcgaccgga ggcgttggca
      781 tattcttcca gcctgccgat gcccccgcga ttcatcgcac ttgcgatcgc tcgcaggtcg
      841 acggtatcgg gcagcgccgc cccggtcatg cccgcataca gctcgagcac gtgcggcccc
      901 caggcgcaca ccagctcgcc ctcgtggaga aatttatcga atgcttcacc gagcgttggc
      961 ccgtcgacac cggccgcgat cgcctccggg gtgagccctg cgatctgtgc ggtgcgcggc
     1021 gcgagcgggc ccgacgggcg cacgaccgcc tcgaagcgct cgcccgtggc catgcggatc
     1081 gccagccagt gcaccagctc gctgcgatag ccgggcacct ggcgctgcgg tgtgtcgtgc
     1141 ggccacgtgt tggcctcgcc gaccacgcat accagctcgc cctcacggcg gtgcagcacc
     1201 tcggtcggca ggatccgcag cgcgcgcggg cggcggttcg tcaggcgctt tcctaaactg
     1261 tgcgcgcagg cgatctgggc gtcgaccatc gcgcggaacg gaattaaaag tgccttgaat
     1321 ttatcggggt cgccctcgag cgcgccgagc accagggcga gcgactcgat cgtcgacacg
     1381 tactcctcgc tcggctcttt acggatccgg tactcgctcg gggccggcgg acgaaacgcg
     1441 tagcggggca ggctcgccag ctccgggttg tcgcggacca gcttgcgcgc gtgggcccag
     1501 gtcccgtcga cgagcaccag cgtcacgggt ccgggaggag gatcggcgat gatgtcgcgg
     1561 gcgcccgggt cggggtacag cagcccgatc ggccgcgacg tgtcggcgag cgcgcgctgc
     1621 aggcccggca tctcggccac gcgcacgccg acgtgcagct cggcgttggg caggcacagc
     1681 cgcgccatgt gggccgtgcc gatcggcatg ccgcgctcgc gcgggtgctg gaccacgatc
     1741 acccgggtcc gcgtcggcag cgagggcagg tgctcgcagt agcagacgcg ggtcggacgc
     1801 aggcacctcg ggcaagtcgg gcgggccaag aggcgcggga ggctaacacg gccgccgccc
     1861 ggcgaaatgc cggcgcgacc cgcggtgctt gcacgagctt gctcatgcaa gatcgccgcg
     1921 tgacacgcga ggcaggtgcg ctacattccc gcgcggagac ggacgatcat gggttttggg
     1981 aagaatccct atgttgccaa ggcgcaggtc gccgagcaaa aggccgaaga ggccgccgac
     2041 gacaacgcgc gcagccgcca ctaccgcgag gccgcccacg agtgggaccg cgccgcggag
     2101 cgcgagaagc cgggcaaggc ccgcagcgag tacgaggccc acgcgctgcg cacccgcgag
     2161 ctcgccggcg agtaattttt tccaaaaagt taaaaccggc cctgcagcac gaccccggcg
     2221 aagccgcggc cgacgctggg ggccgcgctg acgcgtcggc gatgggcgag cacggcgagg
     2281 gtcgtgccgg cgacgaggcc gaccgccccg atgacgccgc cgacgatcag ctgggtgttc
     2341 atcgcctgcc cggcgcggaa gatctcgccg cactcgccga ccggcgcgtc gcggtcgcag
     2401 ccgcgggccg ggtcgtcgaa gcgctgctgc agcgccttgc cgcggaccgc ccccgcgatc
     2461 atcaccccga ggcccgccgc cccgacgccg atcgcagcgc cgccgcccgc cgcgaggccg
     2521 ccccacggcc gcacctcgac ctgccccttc gccaccggcg cgctcgactt cacggtcggc
     2581 ggcgcgaccg gggtcgccgg g
//
LOCUS       DASWNJ010001069         3932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_55292, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001069 DASWNJ010000000
VERSION     DASWNJ010001069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55292"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1188)
                     /locus_tag="VGB85_18020"
     CDS             complement(85..1188)
                     /locus_tag="VGB85_18020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135991.1"
                     /translation="MRTPILAALVLAAACGDTAAPGFSTGPAITSVSVSTSGSSTGAD
                     TTSTGPADDSAGSSASSSTGAATLDVGTVMDFGPAQPPGCKGKVDLLFVISRQGTMGT
                     EQEQLVASFPGFIETIEQTLEGFDVHIMTANPDAYWPGWTCQNQTDGCKNVENWPTCG
                     KDAPDYDCQTFPDMMTPCDEQLGAGLIFNAGGGAANKLCELQDGRRYIVSGEPDMQGA
                     LECIAKVGYAGDTALMGDAMIAALSPTLNGPGGCNEGFLRKDALLVVALITDFHDQGK
                     SYAKQQYEAIVAAKGDPSAVVMLAVVPNAPGEFEPAEDCHYWDEPSFGIFEDLLSRFP
                     YKVFGDTCTPSFAPFFEQAADKISEACGSFIPQ"
     gene            complement(1299..1652)
                     /locus_tag="VGB85_18025"
     CDS             complement(1299..1652)
                     /locus_tag="VGB85_18025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135992.1"
                     /translation="MSDASDASDAVASELERALVQVARAVAELIPGHDVFEPRSLVRC
                     WMARLESEDRGRFLILLLSDKWEAQLGSIAQAVIAGRPVWPAPAPVQASAETMPAEAT
                     AEAMPAAAAPDLANA"
     gene            complement(1796..2743)
                     /locus_tag="VGB85_18030"
     CDS             complement(1796..2743)
                     /locus_tag="VGB85_18030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135993.1"
                     /translation="MFVGFDEPICGGTFDWIESRLRWLADETGLPVAEPSIRYYWLRE
                     DVHRYCDTGACGKTIGDRMYSPLELFTHELVHGHMAQLGVPRPWLAEGMARMFEDTRW
                     SLPEYILTPTAMLEEDEALGLDYDSAASFVGFLRDRFGMPALLELYAALDGVDAEDTP
                     DVFLAVLGEDWDAVESAYLPAYTPGGFVGSLNCEFPVLAPVVDTWTLSVATPCEDAAT
                     VGPYLGWSEEDTPASERHVILEIPVAGTYAVTMTSSAKTSINIEDCGDAPGYFSWYDT
                     QLGEEIELSPGRKRLTITSDIADEAVGEVVLSGPLAPDP"
     gene            complement(2854..3330)
                     /locus_tag="VGB85_18035"
     CDS             complement(2854..3330)
                     /locus_tag="VGB85_18035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135994.1"
                     /translation="MQKTPIGILAGVLLLMAISACGGNGCNEDGDADTAGPAGQECQG
                     PNIANAKYTCPKGFFCEYKSEQAMLNENELGQCKAMEQYKVCKSMTLCGSADFSPKCE
                     TINETAYCDWLQTSVRCKCEGPGPFVEGDADGDDLKTPTSTTPTSTPTTATPTTTQ"
     gene            3356..3517
                     /locus_tag="VGB85_18040"
     CDS             3356..3517
                     /locus_tag="VGB85_18040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135995.1"
                     /translation="MPVPWCCWARECVPAELRAQCMSGGLTLVRAGLTLAELREPAVG
                     PAARRSRAR"
     gene            3514..3663
                     /locus_tag="VGB85_18045"
     CDS             3514..3663
                     /locus_tag="VGB85_18045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135996.1"
                     /translation="MTKLREAANMSGGPTLAARELSVGPYFAIFRISRISRILVAVLD
                     CLAGP"
     gene            3722..>3932
                     /locus_tag="VGB85_18050"
     CDS             3722..>3932
                     /locus_tag="VGB85_18050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135997.1"
                     /translation="MRTMRGRPDMFAGPQLGIDRGGAGPPDMFAAPRSSATAHGSVGP
                     PDMFAGSAARPSCVRASGRRTCSPPR"
BASE COUNT          581 a         1359 c         1328 g          664 t
ORIGIN      
        1 tacatcaccg tcaacggcga gacccaggcc cgcgtcacac ggatccacgg gtaggctcgc
       61 gcggcgcgag gccgtgcagc gaggtcactg cgggatgaag ctcccgcacg cctcgctgat
      121 cttgtccgcc gcttgctcga agaacggcgc gaagctcggc gtacaggtgt cgccgaacac
      181 tttgtagggg aaccgcgaca gcaggtcctc gaagatgccg aacgagggct cgtcccagta
      241 gtggcagtct tctgcgggct cgaactcccc cggcgcgttc gggacgaccg cgagcatgac
      301 gacggcgctc gggtccccct tggccgcgac gatcgcctcg tactgctgtt ttgcgtacga
      361 cttcccctga tcgtgaaagt ccgtgatcaa tgcgacgacg agcagcgcgt ccttgcgcaa
      421 aaagccctcg ttgcacccgc ccggcccgtt cagcgtgggc gagagcgcgg cgatcatcgc
      481 gtcgcccata agcgccgtgt cgccagcgta gccaaccttg gcgatgcact cgagcgcgcc
      541 ctgcatgtcg ggctcgccgc tgacgatgta gcggcggccg tcttgcagct cgcacagctt
      601 gttcgccgcg ccgccgcccg cgttgaagat cagcccggcg ccgagctgct cgtcgcacgg
      661 cgtcatcatg tccgggaacg tctggcagtc gtagtccggg gcgtccttgc cgcaggtcgg
      721 ccagttttcc acgttcttac agccgtcagt ttgattctgg cacgtccagc ccggccagta
      781 ggcgtccggg ttggccgtca tgatgtggac gtcaaagccc tcgagcgtct gctcgatcgt
      841 ctcgatgaag ccggggaagc tggcgacgag ctgctcctgc tcggtcccca tcgtgccctg
      901 gcgcgagatc acgaacagca ggtcgacctt gcccttgcag ccgggcggct gcgcgggccc
      961 gaagtccatc acggtcccga cgtccagcgt cgccgcgcct gtgctcgagc tcgcggagct
     1021 gccggccgag tcatcagcgg gcccggtgga cgtcgtgtcg gcgccggtcg agctgccgct
     1081 cgtcgacacc gacacactcg tgatcgccgg cccggtcgag aacccgggcg cggcagtgtc
     1141 cccgcaggcg gcggcgagga ccagggcggc gaggattggc gtgcgcatcg ccccgcaagc
     1201 ctagtcctgg ccgcgccggc tgtcatcccc aggcgtcccc aggagcccca gcgccggcag
     1261 gtgtttacag cgccaacgcg gcgaacgggg ccacgcgctc acgcattggc gaggtctggc
     1321 gcagcggcag cgggcatcgc ttcggcggtc gcctccgcgg gcatcgtctc ggcgctcgcc
     1381 tgcacgggcg cgggcgcggg ccagacgggc ctgcccgcaa tcacggcttg ggcgatcgag
     1441 ccgagttgcg cctcccactt gtccgacagc agcaagatca ggaagcgccc gcgatcctca
     1501 gactcgagcc gggccatcca gcaacgcacg aggctccgcg gctcgaagac gtcgtgcccc
     1561 ggaatcagct cggcgaccgc gcgggcgacc tgcacgagag cgcgctcaag ctcgctcgcc
     1621 accgcgtcgc ttgcgtcgct cgcgtcgctc atccccccgc cgtgtagcat gttcgtcgtc
     1681 ctcgtgcggc gcactcgctg gcagcggggg gagtggttgg ccgacgcaat tgcggtgcga
     1741 cgtctggcgt ggggcgtggg gagcgccgct ccatgagccc cggtgcggca ggtgatcacg
     1801 ggtccggtgc cagcgggccg ctcagaacga cctcgccgac cgcctcatcg gcgatgtcgc
     1861 tcgtgatcgt gaggcgcttg cggccgggtg acagctcgat ctcctcgcct aactgcgtgt
     1921 cataccagga gaaataaccg ggtgcatcgc cgcaatcctc gatgttgatc gacgtcttcg
     1981 cagaactggt catggtcacc gcgtaggtgc cggccaccgg gatctcgagg atcacgtggc
     2041 gctcgctcgc gggagtgtcc tcctccgacc atccgagata ggggccgacc gtggccgcgt
     2101 cctcgcaagg cgtggcaacg gagagggtcc aggtgtcgac caccggcgcg agcacgggaa
     2161 actcgcagtt gagcgagccg acaaagccgc ccggggtgta cgccggcagg tacgcgctct
     2221 caacggcgtc ccagtcttcg ccgaggacgg cgaggaacac gtccggggtg tcctccgcgt
     2281 cgacgccgtc cagcgcggca tacagctcga gcagggcggg catgccgaag cggtcacgca
     2341 ggaagccgac gaagctggcc gcggagtcgt aatcgagacc cagcgcctcg tcctcctcga
     2401 gcatggcggt gggcgtcaag atgtactcgg gcagggacca acgggtgtcc tcgaacattc
     2461 tggccatccc ttccgccagc cagggccgcg gcacccctag ctgcgccatg tgcccgtgca
     2521 caagctcgtg ggtgaacagc tcgagaggag agtacatccg atcgccgatc gtcttcccgc
     2581 acgcgcccgt gtcgcagtag cgatgaacgt cctcgcggag ccagtagtac cggatcgaag
     2641 gttccgcgac cgggaggccg gtctcgtccg cgagccagcg caggcgtgac tcgatccagt
     2701 cgaaggtgcc gccgcagatc ggctcgtcga agccgacgaa caccgtgatc ccatcgagcg
     2761 ggtactctgg atactctgga tcggcctcac agcctaccgc tccgcaaaga gcgattccaa
     2821 ggacggagcc gagggtcgtg cggcgcatcg ctctcactgt gtcgtcgtcg gtgtcgcggt
     2881 cgtcggcgtg ctcgtcggtg tcgtgctcgt cggtgtcttc agatcatccc cgtccgcgtc
     2941 gccctcgaca aacggccctg gcccctcgca cttgcagcgc acgctggttt ggagccagtc
     3001 gcagtaggcc gtctcgttga tcgtctcgca cttcggggag aagtcggccg acccgcacag
     3061 cgtcatgctc ttgcacacct tgtactgctc catggccttg cattgcccga gttcattttc
     3121 gtttagcatg gcctgttcgc tcttgtactc gcagaaaaat cccttgggac acgtgtactt
     3181 cgcgttggcg atgttggggc cttggcactc ctgacccgct ggccccgccg tgtcagcgtc
     3241 gccgtcctcg ttgcagccgt tgccgccgca ggccgagatc gccatcagca agagaacgcc
     3301 tgcgagaatt ccgatcggtg ttttttgcat cagatagagg tcctcagcta agagtatgcc
     3361 ggttccgtgg tgctgctggg cgcgcgagtg cgtgcccgcc gagcttcgcg cgcagtgcat
     3421 gtccggcggc ctgacgctcg tgcgcgctgg cctgacgctc gccgagctgc gcgagccggc
     3481 ggtcggtccg gcggcccgac gctcccgcgc gcggtgacca agctgcgcga ggcggcgaac
     3541 atgtccgggg gcccgacgct cgcggcgcgc gagctgtcgg tcggccctta ctttgcgatc
     3601 ttcaggatct cccggatctc tcggatcctc gtggccgtcc tcgactgcct ggcggggccg
     3661 taggtcgatc ggtgccgcgc gatagatgac cgagatccgc gggtttccag agatcgcaac
     3721 gttgcgaacc atgcgcgggc ggccggacat gttcgccggc ccgcagctcg gcatcgatcg
     3781 cgggggcgcc ggcccgccgg acatgttcgc cgccccgcgc agctcggcca ccgcgcacgg
     3841 gagcgtcggg ccgccggaca tgttcgccgg ctccgcagct cggccatcgt gcgtgcgagc
     3901 gtcgggccgc cggacatgtt cgccgccccg ca
//
LOCUS       DASWNJ010001070         4163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_478097, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001070 DASWNJ010000000
VERSION     DASWNJ010001070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478097"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VGB85_18055"
     CDS             complement(<1..502)
                     /locus_tag="VGB85_18055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0135998.1"
                     /translation="MAASSSTHTAYPAEVYESFDSFMQQVIKDTYDRGAKRPEFVALV
                     LASGELIPMAWGKMRKSGLRELAFGVGGVVALRYGLAYIVSGPVGLALTGFTVATMIS
                     FFWSNQKAVLARRKPYKQLINDTREKFEDIQARYHDGVYDAGERALMVEGLIRRMLSQ
                     IEAPVEE"
     gene            570..3482
                     /locus_tag="VGB85_18060"
     CDS             570..3482
                     /locus_tag="VGB85_18060"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0135999.1"
                     /translation="MRRPRPSRLARPGHVPARVTTPLRLLAVLLALAPACKDRKAAVP
                     PSAGPEISGENTDPDSAEPGEVEVPELEADAPKKERAKRPPPAPVTTLGAAVDAIGHG
                     NPEGAATFLKDALKKAPADEPLRLTLAHALATTGAYDEAEALLSAKPVKGKAGPRLRM
                     HARLRLLQGDPAGAEKLLDQAIAQDPNDLAARGELLALRVSTGRSNEPSTRALMDGLY
                     DAYDAGKASTAEELVAVARAALARGSSGAFHDANMVLGEAEKLPTPTTGPEQGFIVQD
                     RVLLLRGAVFREKYAASEAADTYNVILARDAWQPDALAGLALAHLEELRLASASESAE
                     AALQTNPRHPDAHGVLARVALVEGRRDEAVTRARSQILTIHPRHPVGLAVLAAAALSS
                     DDDAAFKSARDEALAFSPTAVAFYLALNDVLVSMHLYEQTETVLAEAVKRSPDDPYLQ
                     SAHGLNLLRLGKEPEGRAALARAWKRDRFNERTRNTLDLYEQRIDPNYTDVTSGDLRL
                     RLPKEDPEIVQADLLSAIARARKALDTRYGMHPGPLRIEVFADPEDFSVRTIGVPSLG
                     AVGVCFGDLITSVGPYRGTHNFHQVIWHELAHVYAVKLSRGRVPRWFTEGLSEWESEV
                     ADPSWARESAELLAEARRNGQMRKLGELDLAFLRATSPIMMEVAYATAAWAMRYLGET
                     HGLPKLMLVLKGYSTGETTEALFKKHLGQDMSAVEAGFEAWMNRRLDATISGWQPSPR
                     GKIEARDRTLAKAIEQIKAKDYTGAARTLQVLVQGPGDGFRPRMLLGEVLLQGPQWKS
                     AAPHFEKARSFHREALEPIIRLAELARRSGDLTAEKQHLRAGLEIDAMTFDPAARLTM
                     LAVITDDSKNLAVALERAVAIAPLHPITLGAQALQAARAGDKARAGALGKRALKGVED
                     TEGRGPADTLVVLALASEAAGDLTTAKILATRAAAEKSLPAQAKQAMDRITRITGS"
     gene            complement(3486..>4163)
                     /locus_tag="VGB85_18065"
     CDS             complement(3486..>4163)
                     /locus_tag="VGB85_18065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136000.1"
                     /translation="APGCMREVARWVLPRPWVFGHRRSMSPTIRTILSPCLLACALVG
                     GAGCERVAGFFSEPQAQVESPQRYEKDGISFEYPGNWKLGEETSEENGVTVHNITVES
                     AGNAILMVQTFKPAVPLDLATHFELVMNAMQEEVDKQVGGLADGSRGAVTDFQRQFLG
                     EQRPGKRGEMTVTILGEKVPGVVSMFVAGLEDRTVLLFSTVPDADRAKVEPGFDQVID
                     SLKIATK"
BASE COUNT          669 a         1674 c         1275 g          545 t
ORIGIN      
        1 cctcctcgac cggcgcttcg atctgcgaca gcatgcggcg gatcaggccc tcgaccatca
       61 gggcccgctc gccggcgtcg tagaccccgt cgtggtagcg ggcctggatg tcctcgaact
      121 tctcgcgggt gtcgttgatc agctgcttgt acggcttgcg gcgcgcgagc acggcctttt
      181 ggttgctcca aaagaacgag atcatcgtcg ccacggtgaa gcccgtcagc gcgagaccga
      241 ccgggccgct gacgatgtac gccagaccat aacgcagcgc gaccacacca ccgaccccga
      301 acgccagctc gcgcagcccg ctcttgcgca tcttccccca cgccatcggg atcagctccc
      361 ccgaggccag caccagcgcc acgaactccg gacgcttggc cccgcggtca tacgtgtcct
      421 tgatgacctg ctgcatgaag ctgtcgaagc tctcgtagac ctcggcgggg taggccgtgt
      481 gcgtgctgga ggaagctgcc atggtcgcca gcatagcagc ggaacgccag tttgacagag
      541 cgcgcccgga accgcagaat tcgcggccga tgcgtcgtcc gaggccgtct cgcctggctc
      601 gcccgggcca tgtccccgcg cgggtcacca cgcccctgcg cctgctcgcc gttttgctgg
      661 cgctggcgcc cgcttgcaag gaccgcaagg cggccgtgcc accgagcgcc ggcccggaga
      721 tctccggtga aaataccgac cccgattccg ccgagcccgg cgaggtcgag gtccccgagc
      781 tcgaggccga cgccccgaag aaggaacgcg ccaaacgtcc gccgcccgcg ccggtcacca
      841 ccctcggcgc cgccgtcgac gccatcggcc acggcaaccc cgagggcgcc gccaccttcc
      901 tgaaggatgc cctcaaaaaa gcccccgccg acgagcccct gcgcctgacc ctcgcgcacg
      961 ccctcgccac caccggcgcc tacgacgagg ccgaggcgct cctgtccgca aagccagtca
     1021 agggcaaggc cggcccccgc ctgcgcatgc acgcccgcct gcgcctgctg cagggcgacc
     1081 ccgccggcgc cgagaagtta ctcgaccagg cgatcgcgca ggaccccaac gacctcgctg
     1141 cccgcggcga gctgctcgcc ctgcgcgtca gcaccggccg cagcaacgaa ccgagcaccc
     1201 gcgccctgat ggacggcctc tacgacgcct acgacgccgg caaggccagc accgccgagg
     1261 agctcgtcgc ggtcgcccgc gccgccctcg cccgcggcag cagcggcgcc ttccacgacg
     1321 ccaacatggt gctcggcgag gccgaaaaac tcccgacccc gacgaccggc cccgagcagg
     1381 gctttatcgt ccaggaccgc gtgctgctgc tgcgcggcgc cgtgttccgc gagaagtacg
     1441 ccgccagcga ggccgccgac acttacaacg tgatcctcgc gcgcgacgcc tggcagccgg
     1501 acgcgctcgc cggcctcgcc ctcgcgcacc tcgaggagct gcgcctcgcc agcgccagcg
     1561 agtccgccga ggccgccctg cagaccaacc cgcgccaccc cgacgcccac ggcgtgctcg
     1621 cccgcgtcgc cctcgtcgag ggccgccgcg acgaggcggt cacccgcgca cgcagccaga
     1681 tcttgaccat acacccgcgg cacccggtcg gcctcgccgt gctcgccgcc gccgcgctct
     1741 cgtcggacga cgacgccgct tttaaaagcg cccgcgacga ggccctcgcc ttctcgccga
     1801 ccgccgtcgc cttctacctc gcgctcaacg acgtgctcgt ctcgatgcac ctctacgagc
     1861 agaccgagac cgtgctcgcc gaggccgtca agcgctctcc cgacgacccc tacctgcaat
     1921 ccgcgcacgg cctcaacctg ctccgcctcg gcaaggagcc cgagggccgc gccgcgctgg
     1981 cgcgcgcgtg gaagcgcgac cgcttcaacg agcgcacgcg caacaccctc gacctctacg
     2041 agcagcgcat cgaccccaac tacaccgacg tcaccagcgg cgacctgcgc ctgcgtctcc
     2101 cgaaagaaga ccccgagatc gtccaggccg acctgctctc cgcgatcgcc cgcgcccgca
     2161 aggccctcga cacccgctac ggcatgcacc ccggcccgct gcgcatcgag gtgttcgccg
     2221 accccgagga cttcagcgtg cgcacgatcg gcgtgcccag cctcggcgcc gtcggcgtgt
     2281 gcttcggaga tctgatcacc agcgtcggtc cttaccgagg cacccacaac ttccatcagg
     2341 tcatctggca cgagctcgcc cacgtctacg ccgtcaagct cagccgcgga cgcgtgccgc
     2401 ggtggttcac cgagggcctc agcgagtggg agtccgaggt cgccgacccg agctgggccc
     2461 gcgagtccgc cgagctgctc gcggaggccc gccgcaacgg ccagatgcgc aagctcggcg
     2521 agctcgacct cgccttcctg cgcgccacca gcccgatcat gatggaggtc gcctacgcga
     2581 ccgccgcgtg ggcgatgcgc tacctcggcg agacccacgg cctccccaag ctgatgctcg
     2641 tgctcaaggg ctactcgacc ggcgagacca ccgaggccct cttcaaaaaa cacctcggcc
     2701 aggacatgag cgccgtcgag gccggcttcg aggcgtggat gaacaggcgc ctcgacgcca
     2761 cgatctccgg ctggcagccg agcccgcgcg gcaagatcga agcccgcgac cgcaccctcg
     2821 ccaaggcgat cgagcagatc aaagccaagg actacaccgg cgccgcgcgc acgctgcagg
     2881 tgctcgtcca gggccccggc gacggctttc gcccgcgcat gctgctcggc gaggtgctgc
     2941 tgcaaggacc gcagtggaag tccgccgcgc cgcacttcga gaaggcccgc agcttccacc
     3001 gcgaggcgct cgagccgatc atccgcctcg ccgagctcgc ccgtcgcagc ggcgacctca
     3061 ccgccgagaa gcagcacctg cgcgccggcc tcgagatcga cgcgatgacc ttcgaccccg
     3121 ccgcccgcct gaccatgctc gcggtgatca ccgatgacag caagaacctc gccgtcgcgc
     3181 tcgagcgcgc cgtggcgatc gcaccgctgc acccgatcac cctcggcgcc caggccctcc
     3241 aggccgcccg cgccggcgac aaggctcgcg ctggcgcgct cggcaagcgc gcgctcaagg
     3301 gcgtcgagga caccgagggc cgcggccccg ccgacaccct cgtcgtgctc gccctcgcca
     3361 gcgaggccgc cggcgacctg accaccgcga agatcctcgc cacccgcgcc gccgccgaga
     3421 agagcctgcc tgctcaggcc aagcaggcca tggacaggat cacccgcatc accggctcct
     3481 gactcctact tcgtcgcgat cttgaggctg tcgatcacct gatcgaagcc gggctcgacc
     3541 ttggcccggt cggcgtcggg caccgtgcta aacagcagca ccgtccggtc ctcgaggccg
     3601 gcgacgaaca tcgacacgac acccggcacc ttctcgccga ggatcgtcac cgtcatctcg
     3661 ccgcgcttgc ccggacgctg ctcgccgagg aactggcgct ggaagtccgt caccgccccg
     3721 cggctgccgt cggccaggcc gccgacctgc ttgtcgacct cctcctgcat cgcgttcatc
     3781 acgagctcga agtgcgtcgc gaggtcgagc gggaccgccg gcttgaacgt ctgcaccatc
     3841 aagatcgcgt tgcctgcgct ctcgacggtg atgttgtgca ccgtgacccc gttctcttcg
     3901 ctcgtctcct cgccgagctt ccagttgccg ggatactcga aggagatccc gtccttctcg
     3961 tagcgctgcg gcgactcgac ctgcgcctgc ggctctgaaa agaagcccgc gacgcgttca
     4021 cagcccgcgc ctccgaccag cgcgcaggcg agcagacagg gcgagaggat ggtgcggatc
     4081 gtcggcgaca tcgatcgacg atgcccgaag acccacggcc gcggcaatac ccaccgtgcg
     4141 acctcgcgca tgcaccccgg cgc
//
LOCUS       DASWNJ010001071         3482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_196880, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001071 DASWNJ010000000
VERSION     DASWNJ010001071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196880"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VGB85_18070"
     CDS             complement(<1..405)
                     /locus_tag="VGB85_18070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136001.1"
                     /translation="MQPDPKTRLPDRARHEVVRDRLRSLRLILGLILLLGLASMIGAS
                     KTATPAVVRTHIALWFLAQCSIIVGAVYLLRLVRRLRFGALPSSCLHRTSDGLVYIAA
                     AVGGALALLAFAPRALAGAATGVDWLVVVIGLV"
     gene            complement(395..1576)
                     /locus_tag="VGB85_18075"
     CDS             complement(395..1576)
                     /locus_tag="VGB85_18075"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEY0136002.1"
                     /translation="MGTPTNSDDASRPWDIVVFGATGFVGRLIAAYLDQHAPAQLRLA
                     IAGRDANKLADIAAGLDRPFGKLVASVDDPASLHAMAASARVVLTTVGPYARLGEPVV
                     AACIAAGADYLDLTGEPAFVDRIRATHDEAARKGGSLVIHCCGFDSIPTDLGVLFTIK
                     QLPGGLPITIDGYFEGQASFSGGTFASAVGAMAEGRPARAPRAPAPAGDRVVKRQHER
                     PHYQKALRGWALPLPTIDPQIALRSARARADYGPEFRYGHYMCARSLPRLAVTLAGVA
                     TVALLARTRPTRALLLRLRPSGTGPDEATRGKSWYRFTFFGSAGDTRVVTEVAGGDPG
                     YDETARMAAESALCLALQRHELPHTRGIQTTATAMGEQLLARLQAAGMHFRVIEDSRA
                     T"
     gene            1667..>3482
                     /locus_tag="VGB85_18080"
     CDS             1667..>3482
                     /locus_tag="VGB85_18080"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEY0136003.1"
                     /translation="MSLPAKPQGGPGSVFLIDGSNFMFRAFHALPSMTSPTGIPVNAL
                     HGFVRMIQALRKEFAPEVFVAVFDHAGPTFRNELFPGYKAQRPPPPEDLVPQIPLIRA
                     ATTALGLPAIEARGVEADDIIATYTTQAQRDGRRVIIVSSDKDLMQLVDEGDEKRPAV
                     LLYDTMKNRLLGPAQVCEKFGVPPHQLGDLLALTGDSSDNIPGVPGIGPKTAATLLEE
                     FGNLEGLLAGTAKIKQVKRREVIEANADKARLSRKLVELQREVPDLPPLDSLIDRGSD
                     PEVMTAFFAPLGFKSLAGSARATAAAAGKGPEGPLELRTGAPLVLDRARLVLFRAADE
                     AALIEWIATVTAKASELALQITVDVGDATQAQIVGLAIAPHASDAAVELPPPAYIPVG
                     HTGDALLAGQFTRERGLGLLRPLLAALPTWVHAAKFQAIVLRRFGVELPRLIGDSKLA
                     SYTLDPARTSHTLGELAKDLFAYTVLTPESVLGKGKKAVRFDGAQIDQAAAYACERAA
                     LTGLLGRHLRAQLNASGPEIIALFDELEMPLSRVLEAIEERGIAVDTGVLAAQGKAIG
                     ADIDKLRAEIEAEAGHPLNVDSPLQLQKFLFEERGLEAT"
BASE COUNT          515 a         1184 c         1337 g          446 t
ORIGIN      
        1 gacgaggccg atgacgacga cgagccagtc gacgccggtc gccgctccgg cgagggcgcg
       61 cggggcgaag gcgaggaggg cgagcgcgcc gccgacagcg gccgcgatgt agacgaggcc
      121 gtcgctggtc cggtgcaggc acgaggaggg cagggcgccg aagcggaggc ggcgaacgag
      181 gcgcagcagg tacacggcgc cgacgatgat gctgcactgc gcgaggaacc acagcgcgat
      241 gtgggtgcgg acgacggcgg gcgtggccgt cttgctggcg ccgatcatcg aggcgaggcc
      301 gagcaggagg atcaggccga ggatcagccg caggctgcgc aggcggtcgc gcacgacctc
      361 gtgtcgcgcc cggtccggta acctggtctt cgggtcaggt tgcacggctg tcctcgatca
      421 cgcgaaaatg catgccggcc gcctggaggc gggcgagcag ctgctccccc atcgcggtcg
      481 cggtcgtctg gatcccgcgg gtatgcggga gctcgtggcg ctggagggcg aggcacagcg
      541 cggactcggc ggccatgcgc gccgtctcgt cgtagcccgg gtcaccaccc gcgacctcgg
      601 tgacgacgcg ggtgtcgcca gcgctgccaa agaaggtgaa gcggtaccag ctcttgccgc
      661 gggtggcctc gtcggggccg gtgccggagg ggcgcaggcg cagcagcagc gcgcgggtcg
      721 gccgggtgcg ggcgagcagg gcgacggtcg cgacgccagc gagggtgacg gcgagcctgg
      781 gcagcgagcg cgcacacatg taatgtccgt agcgaaactc ggggccgtag tcggcgcggg
      841 cccgggcgct gcgcagggcg atctgcgggt cgatcgtcgg cagcggcagg gcccagccgc
      901 gcagcgcctt ttgatagtgc gggcgctcgt gctgacgctt gaccacgcgg tcacccgccg
      961 gtgcaggtgc acgcggggcg cgggctgggc gaccctcggc catcgccccg accgccgacg
     1021 cgaaggtccc gccggagaag ctggcctggc cctcgaagta gccgtcgatc gtgatcggga
     1081 gaccgcccgg gagctgcttg atcgtgaaca gcacgccgag gtcggtcggg atgctgtcga
     1141 agccgcagca gtggatcacc agcgagccgc ccttgcgggc cgcctcgtcg tgggtggcac
     1201 ggatccggtc gacgaaggct ggctctccgg tcaggtcgag gtagtcggca cccgcggcga
     1261 tgcaggcggc caccaccggc tcgccgagcc tcgcgtaggg accgacggtg gtcagcacga
     1321 ccctcgcgct ggccgccatc gcgtgcagcg aggccgggtc gtcgacgctg gcgacgagct
     1381 tgccgaacgg gcggtcgagg ccggccgcga tgtcggcgag cttgttcgcg tcgcgcccgg
     1441 cgatcgccag gcgcagctgc gcaggcgcgt gttgatcgag gtaggcggcg atcagtcgcc
     1501 cgacgaagcc ggtcgcgccg aacacgacga tgtcccacgg gcgcgacgcg tcgtccgaat
     1561 tggtaggggt gcccacggcg cggaggatac ccccggaccc ggccgtggtc tcgcgtgacg
     1621 gatcacgggt ggccaagggg ccgtgccggt gctagaaggc gcggtcgtga gcttacctgc
     1681 aaagccccag ggcggtcccg gcagcgtctt tttgatcgac ggcagcaact tcatgttccg
     1741 ggcgttccac gccctgccgt cgatgacctc gccgaccggg atcccggtca acgcgctgca
     1801 cggctttgtc cgcatgatcc aggcgctgcg caaggagttc gcgccggagg tgttcgtcgc
     1861 ggtgttcgac cacgcagggc cgacgtttcg caacgagctg ttccccggct acaaggccca
     1921 gcgcccgccg ccgcccgagg acctggtccc gcagatcccg ttgatccgcg ccgcgaccac
     1981 ggcgctgggc ctgccggcga tcgaggccag gggcgtcgag gccgacgaca tcatcgcgac
     2041 ctacaccacg caggcccagc gcgacggccg gcgcgtcatc atcgtgtcgt ccgacaagga
     2101 cctgatgcag ctcgtcgacg agggcgacga gaagcgcccc gcggtgctgc tctacgacac
     2161 gatgaagaac cgcctgctcg ggccggcgca ggtgtgcgag aagttcggcg tgccgccgca
     2221 ccagctcggc gacctgctgg cgctgacggg cgacagctcg gacaacatcc ccggcgtgcc
     2281 gggcatcggg ccgaagacgg ccgcgacgct gctcgaggag ttcgggaacc tcgaggggct
     2341 gctcgcgggc acggcgaaga tcaagcaggt caagcggcgc gaggtgatcg aggccaacgc
     2401 cgacaaggcg cggctgagcc gcaagctcgt cgagctgcag cgcgaggtgc ccgacctgcc
     2461 gccgctcgac agcctgatcg accgcgggtc cgacccggag gtgatgaccg ccttcttcgc
     2521 gccgctcggc ttcaagtcgc tggccggctc ggcgcgggcg accgccgcgg ccgccggaaa
     2581 gggccccgag gggccgctgg agctgcgtac cggcgcgccg ctggtcctcg accgcgcccg
     2641 cctggtgctg ttccgcgccg ccgacgaggc cgcgctgatc gagtggatcg ccacggtgac
     2701 ggcgaaggcc agcgagctcg cgctgcagat cacggtcgac gtcggcgacg cgacgcaggc
     2761 gcagatcgtc ggcctggcga tcgcgccgca cgcaagcgac gcggccgtcg agctgccgcc
     2821 gccggcgtac atcccggtcg ggcacaccgg ggacgcgctg ctcgccggac agttcacgcg
     2881 cgagcggggg ctggggctgc tgcggccgct gctcgccgcg ctgccgacgt gggtgcacgc
     2941 ggcgaagttc caggcgatcg tgctgcgccg cttcggcgtc gagctgccgc gcctgatcgg
     3001 cgactcgaag ctggcctcgt ataccctcga cccggcgcgc acgagccaca cgctcggcga
     3061 gctcgccaag gacctgttcg cgtacacggt gctgacgccc gagtcggtgc tcggcaaggg
     3121 caaaaaggcg gtccgcttcg acggcgcgca gatcgaccag gcggccgcct acgcgtgcga
     3181 gcgcgcggcg ctgacgggcc tgctcggccg gcaccttcgc gcgcagctaa acgcgtcggg
     3241 accggagatc atcgcgctgt tcgacgagct cgagatgccg ctgtcgcgcg tgctcgaggc
     3301 gatcgaggag cgcgggatcg cggtcgacac gggcgtactg gcggcgcagg gcaaggcgat
     3361 cggcgccgac atcgacaagc tgcgcgccga gatcgaggcc gaggccgggc acccgctcaa
     3421 cgtcgactcg ccgctgcagc tgcaaaaatt cctgttcgag gagcgtgggc tcgaggcgac
     3481 gc
//
LOCUS       DASWNJ010001072         6505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_331810, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001072 DASWNJ010000000
VERSION     DASWNJ010001072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331810"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VGB85_18085"
     CDS             complement(<1..377)
                     /locus_tag="VGB85_18085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136004.1"
                     /translation="MQRLLLCTLLLAACDTDMTVIDVRVYGEEFIESGIPAEVFADGW
                     AVDFDKFLVTVGEVKVTGPGASEVTKPEFQVFDLTQPSDGVGYSVVAGTVREDGRYDD
                     TQFVIAPDPVAAPGNASAADMEVL"
     gene            complement(383..1156)
                     /locus_tag="VGB85_18090"
     CDS             complement(383..1156)
                     /locus_tag="VGB85_18090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136005.1"
                     /translation="MQKLPVLASIFVMMACTPSQEATRVPLGVVLDGSGITETTNDAG
                     WTVTLTTARVAASNLQFSIQGEMHAATASLGGWIVARAWAHPGHYAGGDVTGELIGDF
                     VLDWLGSDGMTLGTADMLAGDYNGMNFTFRAATVADGLAADDPLLGHTAQLAGVARKG
                     DVEVAFTAIVDVDANTQLVGAPFEDTIDAGATAPIEVRFLPTDPVAGTSLFDGLDFAA
                     LDALDKSTDGVAAVSPGDVAHNILRRTLQSHVHYNASPK"
     gene            complement(1162..3129)
                     /locus_tag="VGB85_18095"
     CDS             complement(1162..3129)
                     /locus_tag="VGB85_18095"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0136006.1"
                     /translation="MPRYATVVSTRRPPLLASQRVIDARELSVAPRRRSADDLLRLVP
                     GVLLSQHGAEGKGQQIFLRGFDAAHGSDVEVLVAGIPVNELSNIHGQGYLDLNFMIPE
                     VVRQISVSKGPFQIQQGNFANAGTIRFELGVEPGARGTRVGYELGSTIRHRGVVVHAP
                     SSLGRDSFLALEAMHDQGFAPGRQARRVSSMNQVRLIDSPVYGTLDLLGAGYYATFGS
                     PGALAAADVDAGRIARDGAYQTDGGGSSLRALTSLRHQVSLGRGRLEQRLYGQFRHFD
                     MLENFTGDLQDALHGDRRAQMHRFFSAGYQVEYTRPLAERLTLAVGGAWQGDTISQSD
                     VRVDANHAVVKENWDLGVGQHQAHTRAGLRWRPAQWLTLEGGARADLFAYDVIDRLHD
                     DAREKKLLGRVSPRMLAAFRVGERWSLFTAYGRGLRAPEARAILAGVTTPSDVTLDRY
                     KGGTAKIFPSDAVEVGARFTGKRPVSLGVAAFGTWIDRELVFDHVSAVNLELNQTRRL
                     GVELDATVRPREWLELRQDLTLVHARFVESGAAVPGAPPLLSATTLTLVHRSGIRAGA
                     RLQVVGARPLPHGARAATYALLDLSVGYRRGPLELGVQVDNATNARWKEGEYHYASHW
                     SADDPRSTLPRLHFIAGPPLAVRLATTLHFM"
     gene            3537..4313
                     /locus_tag="VGB85_18100"
     CDS             3537..4313
                     /locus_tag="VGB85_18100"
                     /inference="COORDINATES: protein motif:HMM:NF022135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEY0136007.1"
                     /translation="MTVAAPARADDYWLEFQPLQPSAGSELSLSLWLGKDFIPEAQQA
                     MQQARTVALRHISRAGDTDLLPRTREGVLPLLRLRLGDPGGHLLSLERDTSQVQLRAR
                     TFNRYLENEGLLEVLAERKRAGEMWHRASERYTRYLKAFVQVGDVADGVSTKVVGQRL
                     ELVPERDLAGLKVGERLGMRVLFEGRPLANAQVEAFTRGPSGAPGPAQVASTDSSGRV
                     DLQISEPGTVLVRTVHMQRCVGCGDTQWESFWSVYSFAAR"
     gene            4336..5037
                     /locus_tag="VGB85_18105"
     CDS             4336..5037
                     /locus_tag="VGB85_18105"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY0136008.1"
                     /translation="MTQVQAGDIIQIVRPDGHTAPGQVFPASDPKAPAVVLIQEWWGL
                     NDQIRGVARRIADAGYNVLIPDLYRGKLASDADEAKHLMGDLDFPDALFQDLAGAVHT
                     AKEHHGKVATLGFCMGGALSLAAAIHLQELTSAVVFYGIPPAELADPAKLRVPLLAHF
                     ADHDDWCTPDAVRKLEDELRYSTSEYEIHRYAAQHAFFNEARPEVHDPEAATTAWRRT
                     LAFLARTLGEPSTAA"
     gene            complement(5046..>6505)
                     /gene="priA"
                     /locus_tag="VGB85_18110"
     CDS             complement(5046..>6505)
                     /gene="priA"
                     /locus_tag="VGB85_18110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEY0136009.1"
                     /translation="RARFGDTVAVLHSGLTPRQRLDAWQQIRMGLRPIVIGARSAVFA
                     PVPNLKVIVVDEEHDSSFKQEEGVRYNARDVALVRARDEKALAVLGSATPALETYEQA
                     RAGRYRWLRLDTRPTPRPLPQVELVPLAVHRPDPDTLLTARMRAALTETVLAGEQAIL
                     FLNRRGFATTLVCSICGSLHSCPDCSAPSMTYHLQRNRLMCHLCGHIEAPPEHCRACG
                     QGELLHGSAGTERVELAVTKEIPRARVLRLDRDTSRGKGLIDTLTRFRNREADILVGT
                     QMLSKGHDFPGVTLVGILQADHGLGMPDLRAAERTFQLLTQVSGRAGRGDRAGRVLVQ
                     AWAIAHPAIAFAAEHDFAGFAAWELAQRQALGNPPFGFLALLRISGPDETAVRRRAEA
                     LGAFVRAGVEGAAAKQASLPVGFDSRAPLALLGPVPSPIERVNKKIRWQLLIRATSRQ
                     PLRWLLKQLRPQLGLDGSGDFKTLAVLDVDPQTLL"
BASE COUNT          957 a         2410 c         2223 g          915 t
ORIGIN      
        1 aggacctcca tgtccgccgc gctggcgtta ccgggggccg cgacggggtc cggcgcgatg
       61 acgaattgtg tgtcgtcgta gcgaccgtcc tcccggaccg tgcccgcgac gaccgagtaa
      121 cccacgccgt ccgagggctg cgtcaggtcg aagacctgga actcgggctt cgtcacctcc
      181 gacgcgccgg ggccggtcac cttgacctcg ccgaccgtga ccaggaactt gtcgaagtcg
      241 acggcccagc cgtcggcgaa gacctcggcc gggatgccgg actcgatgaa ttcctcaccg
      301 tacacgcgga cgtcgatcac ggtcatgtcg gtgtcgcagg cggcgagcag gagggtgcag
      361 agcaagagac gttgcatggg gctcacttcg ggctagcgtt gtagtgcacg tgcgactgca
      421 aggtgcggcg caggatgttg tgggcgacgt cgccgggtga gaccgcggcg accccgtccg
      481 tgctcttgtc gagggcgtcg agcgccgcga agtcgaggcc gtcgaacagc gacgtgcccg
      541 cgaccgggtc ggtcggcagg aaccggacct cgatcggcgc cgtcgcgccc gcgtcgatcg
      601 tgtcctcgaa gggcgcgccg accagctgcg tgttcgcgtc gacgtcgacg atcgccgtga
      661 acgcgacctc gacgtccccc ttgcgcgcga ccccggcgag ctgcgccgta tgcccgagca
      721 gcgggtcgtc cgccgcgagc ccgtcggcga ccgtcgccgc gcggaacgtg aagttcatcc
      781 cgttgtagtc gccggccagc atatccgcgg tgccgagcgt catgccgtcg ctgccgagcc
      841 agtcgagcac gaagtcaccg atcagctcgc cggtgacgtc gccgccggcg tagtgccccg
      901 ggtgcgccca cgcgcgcgcg acgatccacc cgccgagcga cgcggtcgcg gcgtgcatct
      961 caccctggat cgaaaattga aggttgctcg cggcgacgcg ggccgtcgtc aacgtgaccg
     1021 tccagccggc atcattcgtc gtctccgtga tccccgagcc gtcgagcacg acccccagcg
     1081 gcacgcgcgt ggcctcctgg ctcggcgtgc aggccatcat gacgaagatg ctcgcaagga
     1141 caggtagctt ttgcatgggt gtcacatgaa gtggagcgtg gtcgccagcc gcaccgcgag
     1201 cgggggcccg gcgatgaagt gcaggcgcgg caaggtacta cgcgggtcgt ccgcgctcca
     1261 gtgcgaggcg tagtggtact cgccctcttt ccagcgcgcg ttggtggcgt tgtcgacctg
     1321 gaccccgagc tcgagcggcc cgcggcgata gccgacgctg aggtcgagca gggcgtaggt
     1381 cgccgcgcgg gccccgtgcg gcagcggccg cgcgccgacg acttgcaggc gcgcgcccgc
     1441 acggatcccg cttcgatgca cgagggtcag cgtcgtcgcg ctcagcaacg gcggcgcccc
     1501 gggcaccgcc gcgccggact cgacgaagcg cgcgtgcacc agcgtcaggt cctggcgcag
     1561 ctcgagccac tcgcgcggac gcactgtcgc gtcgagctcg acgccgaggc ggcgcgtctg
     1621 gttgagctcg aggttgacgg ccgagacgtg gtcgaacacc agctcgcggt cgatccacgt
     1681 gccgaaggcc gcgaccccga gcgacaccgg gcgcttgccg gtgaagcgcg ccccgacctc
     1741 gaccgcgtcg gacgggaaga tcttcgccgt gccgcccttg taacggtcga gcgtgacgtc
     1801 cgacggcgtc gtcacgcccg cgaggatcgc ccgcgcctcg ggggcccgca ggccgcgacc
     1861 atacgcggtg aacagcgacc agcgctcgcc gacgcgaaac gccgccagca tccgcggcga
     1921 gacccgcccg agcagcttct tctcgcgcgc gtcgtcgtgc agccgatcga tgacgtcgta
     1981 ggcgaacagg tcggcccgcg cgccgccctc gagcgtcaac cattgcgccg gccgccagcg
     2041 cagcccggcc cgcgtgtgcg cctggtgctg gccgaccccg aggtcccagt tctccttgac
     2101 cacggcatgg ttcgcgtcga cgcgcacgtc gctctgcgag atcgtgtcac cctgccacgc
     2161 gccgccgacc gccagcgtca gccgctccgc cagcggccgc gtgtactcga cctgatagcc
     2221 ggcggagaag aagcgatgca tctgcgccct gcgatcgccg tgcaaggcgt cctgcagatc
     2281 accagtaaaa ttctcgagca tgtcgaagtg ccggaactgg ccgtacaggc gctgctcgag
     2341 gcgcccgcgc ccgagcgaga cctggtgtct caggctggtc agcgcgcgca atgagctccc
     2401 accgccgtcg gtctggtacg cgccgtcgcg ggcgatccga ccggcgtcga catcggcggc
     2461 ggcgagcgcc ccgggcgatc cgaaggtcgc gtagtagccg gcgccgagca ggtcgagggt
     2521 gccgtacacc gggctgtcga tcagccggac ctggttcatg ctgctgacgc gccgggcctg
     2581 tcgaccgggc gcgaagccct ggtcatgcat cgcctcgagc gcgaggaagc tgtcgcgacc
     2641 gaggctgctc ggcgcatgga cgacgacgcc gcggtgccgg atcgtgctgc cgagctcgta
     2701 gccgacgcgg gtcccgcggg cacccggctc gacgccgagc tcgaagcgga tcgtgcccgc
     2761 attggcaaaa ttgccttgct ggatctggaa cggccccttg ctgaccgaga tctggcgcac
     2821 gacctcgggg atcatgaagt tgaggtcgag gtagccctga ccgtggatgt tcgagagctc
     2881 gttgacgggg atccccgcga ccagcacctc gacgtcggac ccgtgcgccg catcgaagcc
     2941 gcgcaggaag atctgctggc ccttgccctc cgcgccgtgt tggctcagca gcacgcccgg
     3001 caccagccgc agcaggtcgt ccgccgagcg ccgccgcggc gccaccgaca actcgcgcgc
     3061 gtcgatcacc cgctgcgagg cgagcaacgg cgggcgccgc gtgctgacca ccgtcgcgta
     3121 gcgcggcaag gccccgaagc tcggcgtgcg acgcggcgag gacgtcggct cagataccga
     3181 gctttcctcc tctacctgct ccgccgaaga cgggaccgat gtgggtgtgg cgacctgtcc
     3241 caaaggaccg gtcgctgccg ccgcccgcgc aactggcaca gtcgccgcga cgatgccgat
     3301 gaaggcggcc gacctcgtcg ctccgcggat atgcctcagc gccctcaaga ccggtcacgt
     3361 gcctgtgccg tacgctcgcc gagccgcgat agatcgcagg ggcaccacat gcgccgcttg
     3421 cgaaagctcc gcgtcccccg tcatgcttgc gacgataccc catgcagccg ctccctcgcc
     3481 cacgtcgttc gcgcccgtcc ccctgggtcc tcgcgttcgc gctcgcgacc gcggccgtga
     3541 ccgtggccgc ccccgcgcgc gccgacgact actggctcga gttccagccg ctgcaaccga
     3601 gcgccggctc cgagctgtcc ctgtccctgt ggctcggcaa ggacttcatc cccgaggccc
     3661 agcaagcgat gcagcaggcc cgcaccgtcg ccctgcgcca catctcgcgc gccggtgaca
     3721 ccgacctgct cccgcgcacg cgcgagggcg tgctcccgct gctgcgcctg cgcctgggcg
     3781 accccggcgg ccacctgctc tcgctcgagc gcgacacctc ccaggtccag ctgcgcgccc
     3841 gcaccttcaa ccgctacctc gagaacgagg gcctgctcga ggtgctcgcc gagcgcaagc
     3901 gggccggcga gatgtggcat cgcgccagcg agcgctacac gcggtacctc aaggccttcg
     3961 tgcaggtcgg cgacgtcgcc gacggcgtat cgacgaaggt cgtcggccag cgcctcgagc
     4021 tggtccccga gcgcgacctc gcggggctca aggtcggcga gcgcctcgga atgagggtgc
     4081 tgttcgaggg ccgcccgctg gcgaacgcgc aggtcgaggc gtttacgcgc ggcccgagcg
     4141 gcgcgcccgg gccggcgcag gtggcgagca ccgactcgag cggtcgggtg gacctccaga
     4201 tctccgagcc cggcacggtg ctggtgcgca ccgtgcacat gcagcgatgc gtcggctgtg
     4261 gggacacgca gtgggagagc ttctggtcgg tgtacagctt cgccgcgcgc tgagctctcg
     4321 ggtaccatgc cgggcatgac tcaggtccag gcaggcgaca tcatccaaat cgttcgtccc
     4381 gacggccaca ccgccccggg tcaggtcttc cccgccagcg accccaaggc gcccgcggtc
     4441 gtactgatcc aggagtggtg gggcctcaac gatcagatcc gcggcgtcgc ccggcggatc
     4501 gccgacgccg gctacaacgt cctcatcccc gacctctacc ggggtaagtt ggccagcgac
     4561 gccgacgagg ccaagcacct catgggcgac ctcgacttcc ccgacgccct gttccaagac
     4621 ctcgccggcg ccgtgcacac cgcgaaggag caccacggca aggtcgcgac gctcggcttc
     4681 tgcatgggcg gcgcgctgtc cctcgccgcc gcgatccacc tgcaggagct cacctccgcg
     4741 gtcgtttttt atggcatccc gccggccgag ctcgccgacc ccgccaagct gcgcgtgccg
     4801 ctcctcgccc acttcgccga tcacgacgac tggtgcaccc cggacgccgt gcgcaagctc
     4861 gaggacgagc tgcgctactc gacctccgag tacgagatcc accgctacgc cgcgcagcac
     4921 gcgtttttca acgaggcgcg cccggaggtc cacgaccccg aggccgcgac gaccgcgtgg
     4981 cgacgcaccc tcgccttcct cgcgcgcacg ctcggcgagc cgtcgaccgc ggcctgatgc
     5041 tttaattaaa gcagcgtctg cgggtcgacg tcgagcaccg cgagcgtctt gaagtcgccg
     5101 ctgccgtcga ggccgagctg cgggcgcagc tgcttgagca gccagcgcag cggctgccgc
     5161 gaggtcgcgc ggatcagcag ctgccagcgg atcttcttgt tgacccgctc gatcggcgac
     5221 ggcaccggtc cgagcagggc cagcggcgcg cggctgtcaa aaccgaccgg cagcgacgcc
     5281 tgcttggccg ccgcgccctc gacccccgcg cgcacgaatg ccccgagcgc ctcggcccgc
     5341 cgccgcaccg ccgtctcgtc gggcccggag atccgcagca gcgcgaggaa gccgaatggc
     5401 gggttgccga gcgcctggcg ctgcgccagc tcccacgcgg cgaagccggc gaagtcgtgc
     5461 tccgccgcga acgcgatcgc ggggtgagcg atcgcccaag cctgcacgag cacgcggccg
     5521 gccctgtccc cacgccccgc gcgcccgctg acctgcgtca gcagctgaaa ggtccgctcc
     5581 gccgcgcgca ggtccggcat accgagcccg tgatcggcct gaaggatccc gacgagcgtc
     5641 acgcccggga agtcgtggcc cttcgacagc atctgcgtgc cgacgaggat gtcggcctcg
     5701 cggttgcgaa accgggtcaa cgtgtcgatc agccccttgc cgcggctggt gtcgcggtcg
     5761 agccgcagga cccgcgcgcg agggatctcc ttcgtgaccg cgagctcgac ccgctccgtg
     5821 cccgcgctgc cgtgcagcag ctcgccctga ccgcacgccc ggcagtgctc cggcggcgcc
     5881 tcgatgtgcc cgcacaggtg acacatcaag cggttgcgct gcaagtgata cgtcatcgac
     5941 ggcgcgctgc agtccgggca cgagtgcagc gagccgcaga tgctgcacac cagcgtcgtc
     6001 gcgaagccgc gacgattcaa aaataagatc gcctgctcgc cggccagcac cgtctccgtc
     6061 agcgccgcgc gcatgcgggc cgtcagcagc gtgtccgggt ccgggcgatg caccgccagc
     6121 ggcaccagct cgacctgcgg cagcggccgc ggcgtcggcc gggtatcgag gcgcaaccac
     6181 cgatagcggc cggctcgcgc ctgctcgtag gtctccagcg ccggcgtcgc gctgccgagc
     6241 accgcgagcg ccttctcgtc gcgcgcgcgc accagcgcga cgtcgcgggc gttgtagcgg
     6301 acgccctcct cctgcttgaa cgacgagtcg tgctcctcgt cgacgacgat cacttttaaa
     6361 ttaggcaccg gcgcgaacac ggccgagcgc gcgccgatga cgatcggtcg caagcccatg
     6421 cggatctgtt gccacgcgtc gaggcgctgc cgcggcgtca acccgctgtg cagcaccgcc
     6481 accgtgtcgc cgaagcgggc ccgga
//
LOCUS       DASWNJ010001073         1740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_19815, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001073 DASWNJ010000000
VERSION     DASWNJ010001073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19815"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1740
                     /locus_tag="VGB85_18115"
     CDS             <1..>1740
                     /locus_tag="VGB85_18115"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEY0136010.1"
                     /translation="RHRKAYPENATERAHRRLEIGGEYQWRREGEVHLFNPQTVFQLQ
                     HSTRSQKYDVFKQYTSTVDGLSERAGTLRGLFQLKEGVRPPVPLDEVEPVSEIVKRFN
                     TGAMSLGSISPEAHQTLAIAMNRLGGRSNTGEGGEDPERLYDPERRSAIKQVASGRFG
                     VTSEYLVNADDIQIKMAQGAKPGEGGQLPGPKVNPYIAKTRHSTPGVGLISPPPHHDI
                     YSIEDLAQLIHDLKNANDRARIHVKLVAEVGVGTVAAGVSKAHADVVLISGHDGGTGA
                     SPLTSLKHAGSPWELGLAETQQTLLLNGLRDRIVVQCDGQLKTGRDVVIAALLGAEEF
                     GFATAPLVVSGCIMMRVCHLDTCPVGIATQNPVLREKFSGKPEFVVNFMEYIAEEVRE
                     WMARLGFRTMAEMIGHVEVLDTMKAVNHWKAKGLDISPILAVPQNPYGQTLLQSVPQD
                     HGLEAALDVQLIEACEPALERGEQVTLVYPIRNVNRTVGTMLGYEVTRRYGGDGLPDG
                     TIDITFRGSAGQSFGAFVPHGVTLRLVGDANDYVGKGLSGGRIVVRPHADAAFVAQEQ
                     VIAGNTIAYGATSG"
BASE COUNT          290 a          622 c          602 g          226 t
ORIGIN      
        1 gcgccaccgc aaggcctacc cggagaacgc gaccgagcgg gcccaccgca ggctcgagat
       61 cggcggcgag taccagtggc gccgcgaggg cgaggtgcac ctgttcaacc cgcagaccgt
      121 cttccagctg cagcactcga cccgctcgca gaagtacgac gtcttcaagc agtacacctc
      181 caccgtcgac gggctgtccg agcgcgccgg caccctgcgc gggctgttcc agctcaagga
      241 gggcgtgcgc ccgccggtgc cgctcgacga ggtcgagccg gtgagcgaga tcgtgaagcg
      301 gttcaacacc ggggcgatgt cgctcggctc catcagcccc gaggcccacc agacgctcgc
      361 catcgcgatg aaccgcctcg gtggccgcag caacaccggt gagggcggtg aggacccgga
      421 gcgcctgtac gaccccgagc gccgcagcgc gatcaagcag gtggcgtcgg gccgcttcgg
      481 cgtgaccagc gagtacctcg tcaacgccga cgacatccag atcaagatgg cgcagggcgc
      541 caagccgggc gagggcggcc agctgcccgg gccgaaggtc aacccgtaca tcgcgaagac
      601 ccgtcactcg acgcccggcg tcgggctcat ctcaccgccg ccgcaccacg acatctactc
      661 gatcgaggac ctcgcccagc tcatccacga cctgaagaac gccaacgacc gcgcccggat
      721 ccacgtcaag ctcgtggccg aggtcggcgt cggcacggtc gccgcgggcg tgagcaaggc
      781 gcacgccgac gtcgtgctca tctccggcca cgacggcggc accggcgcct cgcccctgac
      841 gagcctcaag cacgccggct cgccgtggga gctgggcctg gccgagacgc agcagacgct
      901 gctgctcaac gggctgcgcg accggatcgt cgtgcagtgc gacggccagc tgaagaccgg
      961 gcgcgacgtc gtcatcgcgg ccctgctcgg ggcggaggag ttcggcttcg cgaccgcccc
     1021 gctcgtcgtg tcgggctgca tcatgatgcg cgtctgccac ctcgacacgt gccccgtcgg
     1081 cattgccacg cagaacccgg tgctgcgcga gaagttcagc ggcaagcccg agttcgtcgt
     1141 gaacttcatg gagtacatcg ccgaggaggt gcgcgagtgg atggcgcgcc tcggcttccg
     1201 cacgatggcc gagatgatcg gccacgtcga ggtgctcgac accatgaagg cggtcaacca
     1261 ctggaaggcg aaggggctcg acatctcgcc gatcctcgcc gtcccgcaga acccgtacgg
     1321 gcagacgctg ctgcagagcg tcccgcagga ccacgggctc gaggcggcgc tcgacgtgca
     1381 gctgatcgag gcgtgtgagc cggcgctcga gcgtggcgag caggtgacgc tcgtgtaccc
     1441 gatccgcaac gtcaaccgca ccgtcggcac gatgctcggc tacgaggtca ctcgccgata
     1501 cggcggcgac ggcctccccg acggcacgat cgacatcacg ttccgcggct cggccggcca
     1561 gtcgttcggc gcgttcgtgc cgcacggggt cacgctgcgg ctggtgggcg acgccaacga
     1621 ctacgtcggc aaggggctct ccggcgggcg gatcgtggtg cggccgcacg ccgacgcggc
     1681 gttcgtggcg caggagcagg tgatcgcggg caacaccatc gcgtacggcg ccacctccgg
//
LOCUS       DASWNJ010001074         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_230927, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001074 DASWNJ010000000
VERSION     DASWNJ010001074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230927"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1755
                     /gene="dnaK"
                     /locus_tag="VGB85_18120"
     CDS             <1..1755
                     /gene="dnaK"
                     /locus_tag="VGB85_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEY0136011.1"
                     /translation="NKGGYRTTPSMVAISESGKRLVGHLAKRQVVTNPVNTVYAAKRL
                     IGRKWNSTAGRTVAQTCPYIIVEGPHGDIRLKIREHVYSVPETSAFILAEMKMIAEQY
                     LGGPVEKAVITVPAYFNDNQRQATKDAGRIAGLEVIRIINEPTAAALAYGFGKNLERK
                     VAVYDLGGGTFDVSILDIGHGVFEVISTAGDTFLGGENFDLRVMQYLIETFEEETGVA
                     IREDRMAMQRLKDAAEKARCELSNVRETVIDLPFLHTTPSGKVFHMHEKLTVQQLEDL
                     TEDLIEDTIKICAKTLKSANLDKGELDDVVLVGGMTRMPMVQRKVAEFFAIEPCKGVH
                     PDEVVALGAAIQAAALIDNTPELLLLDVTPHSLGIMIVGGYFHRLIEQNTTIPTRAGH
                     VFTTVRDNQTSVKILVLQGESDKAAENEMLGEFILSGLRKAPRGQVEVEVTFSISVDG
                     IVSVSAKDLETGREQSITVTATSGLTEDELNDLVKQSQSYMIELRASEESERRRQHAE
                     KLIDTIRGLFPQVQDAMSGTRFGRDAIAKAESVLMRAEQDLARSDSKNLLDTIESLDR
                     TLGMFKNVMHKKSEAAHR"
     gene            complement(1758..>2256)
                     /locus_tag="VGB85_18125"
     CDS             complement(1758..>2256)
                     /locus_tag="VGB85_18125"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY0136012.1"
                     /translation="GGGGMAKLMLGGAIAAYRCYRCADGLELSVGCLEPKFWVAFATL
                     VGRPELAGTGLDPGPAGAATAAAMQTWLSGQPREHWLALLRGQSLPVWPVHETEDAAT
                     EPALALLREATPMPDGSTLDLPGPCLPGLGRTPANPAPALGAHTRAVLTEFGVDPEVV
                     ARLGA"
BASE COUNT          411 a          830 c          724 g          291 t
ORIGIN      
        1 aacaagggcg ggtatcggac cacgccgtcg atggtcgcga tctcggagag cggcaagcgc
       61 ctggtcggtc acctcgccaa gcggcaggtc gtcaccaacc cggtcaacac cgtgtacgcc
      121 gccaagcgcc tgatcggccg caagtggaac tcgaccgccg gccgcaccgt cgcgcagacc
      181 tgcccgtaca tcatcgtcga gggcccgcac ggcgacatcc gcctcaagat ccgcgagcac
      241 gtctacagcg tgcccgagac ctccgcgttc atcctcgcgg agatgaagat gatcgccgag
      301 cagtacctcg gcggcccggt cgagaaggcc gtgatcaccg tcccggccta cttcaacgac
      361 aaccagcgcc aggcgaccaa ggacgccggc cggatcgccg gcctcgaggt catccgcatc
      421 atcaacgagc cgaccgccgc cgcgctcgcc tacggcttcg gcaagaacct cgagcgcaag
      481 gtcgccgtct acgacctcgg cggtggcacc ttcgacgtct cgatcctcga catcggccac
      541 ggcgtcttcg aggtcatctc gaccgccggc gacaccttcc tcggcggcga gaacttcgac
      601 ctgcgggtga tgcagtacct gatcgagacc ttcgaggagg agaccggcgt cgcgatccgc
      661 gaggaccgca tggcgatgca gcggctcaag gacgccgccg agaaggcccg ctgcgagctc
      721 tccaacgtgc gcgagaccgt catcgacctg ccgtttttgc ataccacgcc gagcggcaag
      781 gtgttccaca tgcacgagaa gctgacggtc cagcagctcg aggatctgac cgaggacctg
      841 atcgaggaca cgatcaagat ctgcgccaag accctcaaga gcgccaacct cgacaagggc
      901 gagctcgacg acgtcgtgct cgtcggcggc atgacccgca tgccgatggt ccagcgcaag
      961 gtcgccgagt tctttgcgat cgagccgtgc aagggcgtgc acccggacga ggtcgtcgcc
     1021 ctcggcgcgg cgatccaggc cgccgcgctg atcgacaaca ccccggagct gctgctcctc
     1081 gacgtcaccc cgcacagcct cgggatcatg atcgtcggcg gctacttcca ccgcctcatc
     1141 gagcagaaca ccacgatccc gacccgcgcc ggccacgtct tcacgaccgt gcgcgacaac
     1201 cagacctccg tgaagatcct cgtcctgcag ggcgagagcg acaaggccgc cgagaacgag
     1261 atgctcggcg agttcatcct cagcggtcta cgcaaggcgc cgcgcggcca ggtcgaggtc
     1321 gaggtgacgt tctcgatctc ggtcgacggc atcgtcagcg tcagcgccaa ggacctcgag
     1381 accggccgcg agcagagcat caccgtcacc gccacctccg gcctcaccga ggacgagctc
     1441 aacgacctcg tcaaacagtc gcagagctac atgatcgagc tgcgcgcctc cgaggagagc
     1501 gagcgccgcc gccagcacgc cgagaagctg atcgacacga tccgcggcct gttcccgcag
     1561 gtccaggacg cgatgagcgg cacccgcttc ggccgcgacg cgatcgccaa ggccgagtcc
     1621 gtcctgatgc gcgccgaaca ggacctcgcc cgcagcgaca gcaagaacct gctcgacacg
     1681 atcgagagcc tcgaccgcac cctcggcatg ttcaaaaatg tcatgcacaa gaagtccgag
     1741 gcggcccacc gctgaggtca ggcgccgagg cgggcgacga cctccgggtc gaccccgaac
     1801 tccgtgagca ccgcgcgcgt gtgggccccg agcgcgggcg ccgggttggc aggcgtccgg
     1861 ccgagcccgg gcaagcacgg ccccggcagg tcgagcgtgc ttccatccgg catcggcgtc
     1921 gcttcacgca gcagcgcgag cgccggctcg gtcgcggcgt cctcggtctc gtgcaccggc
     1981 cacaccggca gcgactgccc gcgcagcagc gcgagccagt gttcgcgggg ctgtcccgag
     2041 agccaggtct gcatcgccgc ggccgtcgcc gcgcccgcgg gtccggggtc gaggccggtg
     2101 ccggcgagct cggggcggcc gacgagcgtc gcgaacgcga cccagaactt cggctcgagg
     2161 cagccgaccg agagctcgag cccgtcggcg cagcgatagc agcggtacgc cgcgatcgcg
     2221 ccgccgagca tcaacttcgc catcccgccg ccgccc
//
LOCUS       DASWNJ010001075         2700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_655715, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001075 DASWNJ010000000
VERSION     DASWNJ010001075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655715"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VGB85_18130"
     CDS             complement(<1..672)
                     /locus_tag="VGB85_18130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136013.1"
                     /translation="MRMTARLAVLALSLAACASQPTASNDETITLLRRLHAADPGDPA
                     VLYTFARVRQSLGDYPGTLSWLAQLEASAFDDALDAQDFKRTLRRPEARDLAERLAMR
                     ARVFTPSTVALTIDFPDLMPEGHAHDPRRDRFLLSSGRHRKVVAVDREGRTSDLTRSG
                     QDGLLAVLGMKVDARRDWVWVASTHAPFMVDARPQEHGHSAIHAFDLATGETRAAIPY
                     ARTPSL"
     gene            complement(800..1699)
                     /locus_tag="VGB85_18135"
     CDS             complement(800..1699)
                     /locus_tag="VGB85_18135"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0136014.1"
                     /translation="MLATSTLDRGDTTLHVVKSPAYPHERRAPVLFLHGYPDTHTTWS
                     HQLEGLAAMHPVAAFDMRGVGDSTLPVGPRATAISRYLDDIDAVIDDVAGPGGQVHLV
                     GHDWGGVLGWYIAGDPARARRLRSFSAIAAPHPGAADSMLRGRLRRHGLADLRVLADQ
                     IRRSWYIFLFQIPRIPEWYLTRDVRAMWIRTHRSGGMRRDDPEFAELDPARARSSLIT
                     PLALYRQAFRASRSAPRQVDVPVSLIVPLRDMALRPELYDDVPAFVPDLEQHRLDANH
                     WVHRTRAAEVNQILHDFISRHEP"
     gene            complement(1723..2565)
                     /locus_tag="VGB85_18140"
     CDS             complement(1723..2565)
                     /locus_tag="VGB85_18140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136015.1"
                     /translation="MICTPTTRLFAVSLALSACNQLEKIDESGNASGGDSIPTDVQLA
                     FERSCGTSPACHAAGGLPPTLEGAGIGALIGAPSSAGIPLITLGDTANSYVAIKMLPD
                     DVLGGLGVMRMGARMPLGFDYATGSEDILADTRTILAWIGGADYPGGGTGETADPTDD
                     GGESSSSGGALEPTFANVESTIYATSCSCHYFAPGGPGNGELGLQMGMSYAATVGVKS
                     KQLATMDLIKAGDPEASYLYLKTINKHLDAGGVGDKMPQGGMLSPEQLMLLEEWITAG
                     ALNN"
BASE COUNT          398 a          837 c         1064 g          401 t
ORIGIN      
        1 cagcgagggc gtgcgcgcgt aggggatcgc cgcgcgggtc tccccggtgg cgaggtcgaa
       61 ggcgtggatc gccgagtggc cgtgctcttg cgggcgggcg tcgaccatga acggcgcgtg
      121 ggtcgaggcg acccagaccc agtcgcggcg cgcgtcgacc ttcatgccga gcacggcgag
      181 caggccgtcc tggccggagc gcgtcaggtc gctggtgcgg ccctcgcggt cgacggcgac
      241 gaccttgcgg tggcggccgc tgctgagcag gaagcggtcg cgccggggat cgtgggcgtg
      301 gccctcgggc atgaggtcgg ggaaatcgat ggtcagcgcg acggtgctgg gggtgaacac
      361 gcgggcgcgc atggcgaggc gttcggcgag gtcacgcgcc tcggggcggc gcagcgtacg
      421 cttgaagtcc tgggcgtcga gcgcatcgtc gaaggcggag gcctcgagct gcgcgagcca
      481 cgagagcgtg ccggggtagt cgccgaggga ctggcgcacg cgggcaaagg tgtagagcac
      541 cgcggggtcg ccgggatccg cggcgtggag gcgtcggagc agggtgatcg tttcgtcgtt
      601 cgacgccgtg ggctggctgg cacaggcggc gagcgacagg gcgagcacag cgaggcgggc
      661 agtcatacgc atggggcttg gagaccacga gaccataaat tagtttcgcc gtcgcggcgc
      721 gccgcaaaca tgacgctgtc aacctcgccg tgcgccgccg gctccgcgcg gtgaaaccaa
      781 gcccgcgatc cggcggtcgt cagggttcgt gccgcgagat gaagtcgtgg aggatctggt
      841 tgacctccgc ggcgcgtgtg cgatgcaccc agtggttggc gtcgaggcgg tgctgctcga
      901 ggtccggcac gaacgcgggc acgtcgtcgt agagctccgg gcgcagggcc atgtcgcgca
      961 gcggcacgat gaggctcacc ggcacgtcga cctgacgcgg ggcggaccgc gaggcgcgaa
     1021 acgcctggcg gtagagcgcg agcggggtga tcaggctgct gcgcgcgcgc gccgggtcga
     1081 gctcggcgaa ctcggggtcg tcgcgccgca tgccgccgga gcgatgcgta cggatccaca
     1141 tcgcgcgcac gtcgcgggtc aggtaccact ccgggatccg cgggatctgg aacaggaaga
     1201 tgtaccagga gcgccggatc tggtcggcga gcacgcgcag gtcggcgaga ccgtgccggc
     1261 gcaggcggcc gcgcagcatc gagtcggcgg cccccgggtg cggggcggcg atcgctgaaa
     1321 aactgcgcag gcgcctggcg cgggccggat cgccggcgat ataccagccg agcacgccgc
     1381 cccagtcgtg accgacgagg tggacctgcc cgccgggacc ggcgacgtcg tcgatcacgg
     1441 cgtcgatgtc gtcgaggtag cgcgagatcg cggtcgcgcg tgggcccacc ggcaaggtcg
     1501 agtcaccgac gccacgcatg tcgaaggcgg cgaccgggtg catcgccgcg aggccctcga
     1561 gctggtgcga ccaggtcgtg tgggtgtcgg ggtagccgtg caggaacagc accggcgcgc
     1621 gccgctcgtg cgggtaggcc gggctcttga cgacgtgcag cgtggtgtcg ccgcgatcga
     1681 gggtggaggt cgcgagcatg gcttcccttt tttaagaatt gctcagttgt tgagcgcgcc
     1741 ggcggtgatc cactcctcga gcaacatgag ctgctcgggc gagagcatgc cgccctgggg
     1801 catcttgtcg ccgacgccgc cggcgtcgag gtgcttgttg atcgtcttga ggtacaggta
     1861 gctggcctcg gggtcgccgg ccttgatcag gtccatcgtc gccagctgct tcgacttcac
     1921 gccgacggtc gccgcgtagg acatgcccat ctgcaggccg agctcgccgt tgccggggcc
     1981 cccgggcgcg aagtagtggc agctgcacga ggtcgcgtag atcgtgctct cgacgtttgc
     2041 gaaggtcggc tcgagcgcgc cgcccgagct gctcgactcg ccgccatcgt cggtcgggtc
     2101 cgcggtctcg ccggtgccac caccggggta atcggcgccg ccgatccacg cgaggatcgt
     2161 gcgggtgtcg gcgaggatgt cctcgctgcc ggtcgcgtag tcgaagccga gcggcatgcg
     2221 ggcgcccatg cgcatcacac cgagcccgcc gagcacgtcg tccggcagca tcttgatcgc
     2281 cacgtacgag ttggcggtgt cgccgagagt gatcagcggg atgccggccg acgagggcgc
     2341 gccgatcaac gcgccgatgc cggcgccctc gagcgtcggc gggaggccgc cggccgcgtg
     2401 acaggcgggc gaggtgccgc agctgcgctc gaaggcgagc tgcacgtcgg tggggatgct
     2461 gtcgccgccg ctggcgttgc cgctctcgtc gatcttctcg agctgattac acgccgagag
     2521 cgcgagcgac acggcgaaca aacgggtcgt gggggtgcag atcatggcgc tacaggctcc
     2581 actgtcggct cagattaaaa ccgaagaaaa agttaccgcg atcggcgaag gggttcttcg
     2641 actggccgcc gggggcatag agattggtgt ggatctcgcg ggtgttggtg aggaagatct
//
LOCUS       DASWNJ010001076         4173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_268122, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001076 DASWNJ010000000
VERSION     DASWNJ010001076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268122"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VGB85_18145"
     CDS             <1..660
                     /locus_tag="VGB85_18145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136016.1"
                     /translation="QAGDTTLKGGTPASAGVLAALNHLKTLDPGVPRAILLVTDGAAN
                     CAMGAPVPGLFESYDQNLHTIVKAALADDGIPTYVVGIDTKDVVSDNAQDGNPNGTNT
                     YQKLNELAVDGGKPKDDPNEKFYNAVNQIQLKAALDIIAADALSCTIPLETEPAFPNA
                     TKVIVGGKEVPMVTDCATESGWVYDNPMGPFTAVILCGTACEDLKVAKAADVEYYCTA
                     G"
     gene            1113..3167
                     /locus_tag="VGB85_18150"
     CDS             1113..3167
                     /locus_tag="VGB85_18150"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HEY0136017.1"
                     /translation="MSPVMLCLAACGDDGRADTAATTTNPTMVTTITSAGSTTGTADP
                     TEATDPTANPTGPTGSTSGGTPGTESQGDTSGSETTDPGTTETTVTTGPGGCGVCPEG
                     QFCNVDVCEPGCDGDEDCSAPAVCDLGSHTCKGCLDDAGCALGTVCTDGTCVPGCSDT
                     QPCNDGLACCAGGCFDLLADPLHCGGCEAPCPVPANAMATCSESMCGLGACADGFANC
                     DGDVANGCEVSGDCQCTPGEQTACYSGPDGTQDVGVCKGGLQTCNAQGTGFGACMGEV
                     LPGGEDICGNGLDDNCNGVKDEDPDADGDGWTVCGGDCCDAVGPNCLNPELVNPGAFE
                     VMGNMVDDDCDSKQDNVVPACDNGLASNSADPLEYAKAIDLCQFTIENPPLKDKKWGV
                     ISGLFSRSNGAGNPAAVARSIRDGFGANVPPKANTRLAVLSTGNAADASDTNPNFAKF
                     QGGTDLSSEAGVPADWLALNNNNFPNVAGCPEPQGGNTGNDTIQLKLRIRVPTNAQSF
                     NTKIYFFSSEYPEWVCSAFNDFFVTLVDSGGAGNPTDKNIAVYKDNQNKLFPVGVNLV
                     KTANGLFTQCKNGAIGCGGGAVPGNYAGCVNNNELIGTGFDPLNPAPQFANDPGYCGS
                     NNQVGGGTGWLKMSGNVKPGETMEIRFTIWDTGDGFYDSVVLLDDFQWSLQASQPGVQ
                     PG"
     gene            complement(3878..>4173)
                     /locus_tag="VGB85_18155"
     CDS             complement(3878..>4173)
                     /locus_tag="VGB85_18155"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0136018.1"
                     /translation="EFMLAGLATLPGVDTFPPDGAFYLYADISRHLGPGTQFADDLAL
                     AGWLLEQKMVATVPGTAFGTPGFLRISYATDDESLRTGLTRIQEAFATLPTAR"
BASE COUNT          727 a         1546 c         1356 g          544 t
ORIGIN      
        1 caggccggcg acaccacgct caagggcggc accccggcct ctgcgggcgt gctcgcggcg
       61 ctcaaccacc tcaagacgct cgacccgggc gtcccgcggg cgatcctcct cgtcaccgac
      121 ggcgcggcca actgcgcgat gggtgccccg gtcccgggcc tgttcgagag ctacgatcag
      181 aacctgcaca cgatcgtgaa ggccgccctc gccgacgacg gcatcccgac ctacgtcgtc
      241 ggcatcgaca ccaaggatgt cgtcagcgac aacgcgcagg acggtaaccc caacggcacc
      301 aacacctacc agaagctcaa cgagctcgcg gtcgacggtg gcaagcccaa ggacgacccg
      361 aacgagaagt tctacaacgc ggtcaaccag atccagctca aggccgcgct cgacatcatc
      421 gccgccgacg ccctcagctg cacgatcccg ctcgagaccg agccggcctt ccccaacgcg
      481 accaaggtga tcgtcggtgg caaggaagtc ccgatggtca cggactgcgc cacggagagc
      541 ggctgggtct acgacaaccc gatgggcccg ttcaccgccg tcatcctgtg cggcaccgcc
      601 tgcgaggacc tcaaggtcgc caaggccgcg gacgtcgagt actactgcac cgccggctga
      661 gcccgggcat ccggacatcc gaacgcatcc gagcggctgg cagcctcgct gtcagccgct
      721 cttttgcgtc cggggtcgaa catcgggcgc tcagccgcag cggtcggcga cgcagctgcc
      781 agccgagcag accttactga cgatgcgcct cgagccactg tcactaccga cgcaagtaac
      841 ccgcgcccga cgtccatgac ggcctgcgcg gcgcgtcacg gccccgagaa ctcggccgga
      901 atggtaaggt gggtgccgct gggcgacctc ctcgcccact gtcagattca tcgtcgaaca
      961 ggagcagccc ggggtggcga gacagtggag ggaacgggcg cggcgccgcg ggcactcctg
     1021 ggtccagcgc gattgtgatt gcgggacgca cacgggcatg cctatggtga aatatgcgcc
     1081 cctcttcact tggtcttgcg agcctcgccc tcgtgtctcc ggtcatgctg tgcctcgccg
     1141 cctgcggcga cgacggccgg gccgacaccg cggcgacgac caccaacccc accatggtca
     1201 cgacgatcac gtcggccggc agcacgacag gcaccgccga cccgaccgag gccaccgacc
     1261 cgaccgccaa ccccacgggc ccgaccggtt cgacgtccgg cgggacaccg ggcacggagt
     1321 cgcagggcga caccagcggc tcggagacga ccgaccccgg caccaccgag acgaccgtca
     1381 ccaccgggcc cggcggctgc ggcgtctgtc ccgagggaca gttctgtaac gtcgacgtct
     1441 gtgagcccgg ctgcgacggc gacgaggact gcagcgcgcc cgcggtgtgc gacctcggca
     1501 gccacacctg caagggctgc ctcgacgacg ccggctgtgc gctcggcacc gtgtgcaccg
     1561 atggcacgtg cgtgcccggg tgcagcgaca cgcagccgtg caacgacggc ctggcctgct
     1621 gcgcgggcgg ctgcttcgac ctgctggcgg acccgctgca ctgcggcggc tgcgaagcgc
     1681 cctgcccggt cccggccaac gcgatggcga cctgcagcga gagcatgtgc ggcctcgggg
     1741 cctgcgccga cggcttcgcc aactgcgacg gcgacgtcgc caacggctgc gaggtcagcg
     1801 gtgactgcca gtgcaccccg ggcgagcaga ccgcgtgcta ctccggcccc gacggcaccc
     1861 aggacgtcgg cgtctgcaag ggcggcctac agacctgcaa cgcgcagggc accggcttcg
     1921 gcgcgtgcat gggcgaggtc ctgcccggcg gcgaggacat ctgcggcaac ggcctcgacg
     1981 acaactgcaa cggcgtcaag gacgaggacc ccgacgccga cggcgacggc tggaccgtgt
     2041 gcggcggcga ctgctgcgac gccgtcggcc ccaactgcct caaccccgag ctcgtcaacc
     2101 ccggtgcctt cgaggtgatg ggcaacatgg tcgacgacga ctgcgacagc aagcaggaca
     2161 acgtcgtccc ggcctgcgac aacggcctgg cgagcaactc cgcggacccg ctcgagtacg
     2221 ccaaggcgat cgacctgtgc cagttcacga tcgagaaccc gccgctcaag gacaagaagt
     2281 ggggcgtgat cagcgggctg tttagtcgct ccaacggcgc gggcaacccc gccgcggtcg
     2341 cgcgatcgat ccgcgacggc ttcggcgcca acgtgccgcc caaggccaac acgcggctcg
     2401 ccgtgctgtc gaccggcaac gcggccgacg cctccgacac caacccgaac ttcgccaagt
     2461 tccagggcgg caccgacctc agcagcgagg ccggtgtgcc ggccgactgg ctcgcgctca
     2521 acaacaacaa cttccccaac gtcgccggct gccccgagcc gcagggcggc aacaccggca
     2581 acgacaccat ccagctcaag ctgcggatcc gcgtgccgac caacgcgcag tcgttcaaca
     2641 ccaagatcta cttcttctcc tccgagtacc cggagtgggt ctgcagcgcc ttcaacgact
     2701 tcttcgtcac gctcgtcgac tccggcggcg ccggcaaccc gaccgacaag aacatcgccg
     2761 tctacaagga caaccaaaac aagctgttcc cggtcggcgt caacctggtc aagaccgcca
     2821 acggcctgtt cacgcagtgc aagaacggcg cgatcggctg cggcggcggt gccgtgcccg
     2881 gcaactacgc cggctgcgtc aacaacaacg agctgatcgg caccggcttc gaccccctca
     2941 accccgcccc ccagttcgcc aacgaccccg gctactgcgg cagcaacaac caggtcggcg
     3001 gcggcaccgg ctggctcaag atgtccggca acgtcaaacc gggcgaaacg atggagatcc
     3061 gcttcacgat ctgggacacc ggcgacggct tctacgactc ggtcgtgctg ctcgacgact
     3121 tccagtggtc gctgcaggcg tcgcagcccg gcgtgcagcc ggggtgatgc gggcccgaac
     3181 gagacggatg tttggccggt gatgcctctg ccggggggac gggggacccc cggccggtcg
     3241 tcgttgccac cagcagcggc gcttcgcttg ctgctggcgg catccgataa ccgtccgggg
     3301 gtcccccgtc cccccggcac ggcttcgccg tgaaacaagc gtcgttcggc ctggcctcgt
     3361 tcagcaccgt cggcgtgcaa aggacgtcag cgcccgcgac ccgctcgttc ctcgcggctc
     3421 gcggacgctg cgtgtgcgta cgcgatcgat gcctccgacg ttcttcgcgg cgcgccttgc
     3481 gtgctcgaca tgtcccgaag gacaccttgc cacccgccac cccgccaccc ggccacccgg
     3541 ccatccgcac acaggcagga gacccaccga gacgatgccc gcgcagcacg tggccggcga
     3601 acacgctctc cgccgacgca ggatccgacg ggcggtcgaa gaccgacagt cggatcccgg
     3661 gcctctatcc ggcgacgtcc cgcgatcggg cgaggccggc cacgcatgtt ccacggggtc
     3721 gcatgccccg gggggtcggg gttccctcgc ggagatttcg gggccgtgag gacgagcgaa
     3781 gcgaagtcct catgaagcct cgaaatctcc gcgagggaac cccgaccccc cgaaagcagc
     3841 gtctccggca ggacatccgc cgccgtttca cgccgctcta gcgcgccgtc ggaagcgtcg
     3901 cgaacgcctc ctggatgcgc gtaagtccgg tccgcaggct ctcatcatcg gtcgcgtacg
     3961 agatccgcaa gaacccgggc gtcccgaacg cggtgcccgg cacggtggcg accatcttct
     4021 gctcgagcag ccagcccgcg agcgcgaggt cgtccgcgaa ttgcgtgccc ggcccgaggt
     4081 gccgcgagat atcggcgtag agatagaagg cgccgtcggg cgggaaggtg tcgacgccgg
     4141 gcagcgtggc gagccccgcg agcatgaatt cgc
//
LOCUS       DASWNJ010001077         2739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_584894, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001077 DASWNJ010000000
VERSION     DASWNJ010001077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584894"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VGB85_18160"
     CDS             <1..378
                     /locus_tag="VGB85_18160"
                     /inference="COORDINATES: protein motif:HMM:NF039450.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADYC domain-containing protein"
                     /protein_id="HEY0136019.1"
                     /translation="CVEYKVGKKTKKFCWDVSLKDHHQACTRMARADYCGTGQSWTVP
                     GTPIDIYDALDPRIETAETDWPVEAEWNPNGAYCLDDIRQQGWKAEGKYPKCKWPLAR
                     EKSDCGSLKDHRALLVSKFEPVD"
     gene            486..1442
                     /locus_tag="VGB85_18165"
     CDS             486..1442
                     /locus_tag="VGB85_18165"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY0136020.1"
                     /translation="MPTVEFDREKTVEVEDLDLTLLEIAREHGIPHASACGGNGRCST
                     CRVMILEHPENLDPRNAVEARLARAKGFEDNIRLACQTTITGSVKLRRLVIDEEDLAI
                     AQDGTPQTIGKDRQLAILFSDIRNFTPFAESHLPYDVIHILGRYFRKVGEPVIRHGGY
                     IDKYMGDGIMAIFGLERATATEACRDAVAAALGMLDSLRELNVYISKQFDTEFRVGIG
                     IHVGSVIIGEMGHPQKMQFTAIGDVVNIASRIESATKEVGVPLLVSAEVRALLGDDVR
                     WGVSQRVNLKGKSGEHLLSEVTGLNIEVPETTPLPGVTAPVP"
     gene            complement(1445..>2739)
                     /locus_tag="VGB85_18170"
     CDS             complement(1445..>2739)
                     /locus_tag="VGB85_18170"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0136021.1"
                     /translation="YRPPARRGDCLRMAIQRRTFLRYAGGLAGLAACRPTAEPAHPPP
                     GPGPEVARSPATDPPLAPEPILLGGEKLKILVLGGTGFLGPHLVEAMLQRGHTPTLFN
                     RGKTRPGLFPDVEKLRGDRDGGLQPLQGRRWDAVIDTSGYVPRVVRASAELLAPNVRQ
                     YVFVSSISVYADTTTPNPDESHPVATMPDPKSEAVMEFYGALKALCEQAVEAALPGRT
                     TNIRPGLIVGPGDPTDRFTYWPVRVAQGGEVLAPGNPNDPVQFIDARDLAAFILTTLE
                     KGHVGVYNATGPAEPLGIEGLLTACKTVSGSDASFTWADGEFLAAQKVEAWSDMPVWV
                     PPVGDGIGMATTSVARAVGKGLSFRPLADTVKATLDWWATLPADRRAKLRAGITRERE
                     AEVLAAWHKAHPPARPVKPVKPVRGKAKPKASAAAAAG"
BASE COUNT          409 a          933 c         1003 g          394 t
ORIGIN      
        1 tgcgtcgagt acaaggtcgg caagaagacc aagaagttct gctgggacgt ctcgctcaag
       61 gaccaccacc aggcctgcac ccgcatggcc cgcgccgact actgcggcac cggccagtcg
      121 tggaccgtgc ccggcacgcc gatcgacatc tacgacgccc tcgacccgcg catcgagacc
      181 gccgagaccg actggcccgt cgaggccgag tggaacccca acggcgcgta ctgcctcgac
      241 gacatccgcc agcagggctg gaaggccgag ggcaagtacc cgaagtgcaa gtggccgctg
      301 gcccgcgaga agagcgactg cggcagcctc aaggaccacc gcgcgctgct cgtcagcaag
      361 ttcgagccgg tcgactgagc acgggcgagc gcgctcgctc ttgcggatcg ctttgagatc
      421 acgggcctgg cggcgacgcc gggccttttt catggggcgc cggccctggt gtacgcttgc
      481 gcggcatgcc gaccgtcgag ttcgaccgcg agaagaccgt cgaggtcgag gacctcgacc
      541 tcacgctgct cgagatcgcc cgggagcacg gcatccccca cgcgagcgcc tgcggcggta
      601 acggccgctg ctcgacctgc cgcgtgatga tcctcgagca cccggagaac ctcgaccctc
      661 gcaacgccgt ggaggcccgc ctggcccgcg ccaagggctt cgaggacaac atccgcctcg
      721 cctgtcagac gacgatcacc gggtccgtca agctgcgccg cctcgtgatc gacgaggagg
      781 acctggcgat cgcccaggat ggcacgccgc agacgatcgg caaggaccgc cagctggcga
      841 tcctcttcag tgacatccgc aacttcacgc ccttcgccga gagccacctg ccgtacgacg
      901 tcatccacat cctcggcagg tactttcgca aagtgggaga gccggtgatc cgccacggcg
      961 gctacatcga caagtacatg ggcgacggga tcatggcgat cttcggcctc gagcgcgcga
     1021 ccgcgaccga ggcctgtcgc gacgccgtcg ccgcggcgct cggcatgctc gacagcctgc
     1081 gcgagctcaa cgtgtacatc agcaagcagt tcgacaccga gtttcgcgtc ggcatcggga
     1141 tccacgtcgg ctcggtgatc atcggcgaga tgggtcaccc acagaagatg cagttcacgg
     1201 cgatcggcga cgtcgtcaac atcgccagcc gcatcgagtc ggcgaccaag gaggtcggcg
     1261 tgccgctgct ggtctcggcc gaggtgcggg cgctgctcgg cgacgatgtc cggtgggggg
     1321 tgtcgcagcg cgtcaacctc aagggcaagt ccggcgagca cctgctctcg gaggtgaccg
     1381 gcctcaacat cgaggtcccc gagacgacgc cgctgcccgg cgtgacggcg ccggtgccct
     1441 gagctcagcc ggcggccgcg gccgcgctgg cctttggttt ggccttgccc ctgacgggct
     1501 tgacgggctt gacgggcctg gcgggcgggt gcgccttgtg ccacgcggcc aggacctcgg
     1561 cctcgcgctc gcgggtgatg ccggcgcgca gcttggcgcg gcggtcggcc ggcagggtgg
     1621 cccaccagtc gagcgtcgcc ttgacggtgt cggcgagcgg gcgaaagctc aggcccttgc
     1681 cgaccgcgcg cgcgacgctg gtggtcgcca tgccgatgcc gtcgccgacg ggcggcaccc
     1741 acaccggcat gtcgctccac gcctcgacct tttgcgcggc caggaattcg ccgtcggccc
     1801 aggtgaagct ggcgtcgctg ccggacacgg tcttgcaggc cgtcagcagg ccctcgatgc
     1861 cgagcggctc ggccgggccg gtcgcgttgt agacgccgac gtggcccttc tcgagggtgg
     1921 tgaggatgaa ggccgcgagg tcgcgggcgt cgatgaactg gaccgggtcg ttggggttgc
     1981 cgggggcgag cacctcgccg ccctgggcga cgcgcacggg ccagtaggtg aagcggtcgg
     2041 tcgggtcacc ggggccgacg atgaggcccg ggcggatgtt ggtggtgcgg ccgggcagcg
     2101 cggcctcgac ggcctgctcg cacagggcct tgagcgcgcc gtagaactcc atcacggcct
     2161 cggacttggg gtcgggcatg gtcgcgaccg ggtgcgactc gtcggggttg ggcgtggtcg
     2221 tgtcggcgta caccgagatc gacgagacga acacgtactg gcgcacgttc ggcgccagca
     2281 gctcggccga ggcgcggacc accctcggca cgtagccgga ggtgtcgatg acggcgtccc
     2341 agcggcggcc ttgcagcggc tgcaggccgc cgtcgcgatc tccgcgcagc ttctcgacgt
     2401 cggggaacag gccggggcgg gtcttgccgc ggttgaacag ggtgggggtg tgcccgcgct
     2461 gcagcatcgc ctcgacgagg tgcgggccga ggaagccggt gccaccgagc acgaggatct
     2521 tgagcttctc gccgccgagc aggatcggct cgggcgccag cggtgggtcc gtggcggggc
     2581 tcctggcgac ctcggggccc ggccccggcg gtgggtgggc cggctcggcg gtgggccgac
     2641 acgcggccag accggcgagg ccgccggcat atcgcagaaa ggttcgtcgc tggatcgcca
     2701 tgcgcaagca gtctcctctg cgcgcggggg gcctgtaca
//
LOCUS       DASWNJ010001078         1535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_402069, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001078 DASWNJ010000000
VERSION     DASWNJ010001078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402069"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1473
                     /locus_tag="VGB85_18175"
     CDS             <1..1473
                     /locus_tag="VGB85_18175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136022.1"
                     /translation="ADDALGYVADAEGNRIPDFSYAGYHRGEAPIPDVPTVTKIGPIA
                     DDNTKHIQDAIDMVGKMPLGPDGLRGAVELEPGTYTILGTILLRHSGVVLRGAGDGDD
                     PATSTILRAVGDVPHQRDVVVVGSGSNNKWVPEIADTKTDIVSERVEVGERQLEVADP
                     DNFQVGDHVVIFHPCTAKWLAAIDGGGTGTDADWSPSSLPIVFKRQIMAINGATLTID
                     APVYNHLDRSLSQSYMYKLDRKNILTELGVEDLRIDIQTKGGTDENHAWNAVAMRGLE
                     DGWVRRFTALHFGHSAVIVQTGTQITVADSRGIDPVAQITGERMYNFDVSNGQQVLFT
                     GCHASKGRHHFVSNGTTWTSGVVFHRSTSTGAYAMSEGHRQWSTGMLYDNIIESDLNK
                     DGMTALALYNHGDYGTSHGWGNAHSVAWNYDVSTGRGLIQRPPTAQNYAIGGAGTFAG
                     TKPPAPFDHPEGHIEGIGKPGLVPESLYERQLAERLCGLR"
BASE COUNT          282 a          560 c          500 g          193 t
ORIGIN      
        1 gccgacgacg cgctcggcta cgtcgccgac gccgagggca accgcatccc ggacttcagc
       61 tacgcgggct accaccgcgg cgaggcgccg atccccgacg tgccgaccgt gacgaagatc
      121 gggccgatcg ccgacgacaa caccaagcac atccaggacg cgatcgacat ggtcggcaag
      181 atgccgctcg gccctgacgg cctgcgcggc gcggtcgagc tcgagcccgg cacgtacacg
      241 atcctcggca cgatcctgct gcgccacagc ggcgtggtcc tgcgcggcgc gggcgacggc
      301 gacgacccgg cgaccagcac gatcttgcgg gcggtcggcg acgtgccgca tcaacgcgac
      361 gtcgtggtgg tcggcagcgg cagcaacaac aagtgggtcc ccgagatcgc cgacacgaag
      421 accgacatcg tcagcgagag ggtcgaggtc ggcgagcgcc agctcgaagt cgccgatccc
      481 gacaacttcc aggtcggcga ccatgtggtg atcttccacc cctgcaccgc gaagtggctc
      541 gccgcgatcg acggcggcgg caccggcacc gacgccgact ggagcccgag ctcgctgccg
      601 atcgtgttca agcgccagat catggcgatc aacggcgcga cgctgacgat cgacgccccg
      661 gtgtacaacc acctcgatcg ctcgctgtcg cagtcttaca tgtacaagct cgaccgcaag
      721 aacatcctga ccgagctcgg cgtggaggac ctgcggatcg acatccaaac caagggcggc
      781 accgacgaga accacgcgtg gaacgcggtc gccatgcgcg ggctcgagga cggctgggtc
      841 cgtcgcttca ccgcgctgca cttcggccac tccgcggtca tcgtgcagac cgggacccag
      901 atcacggtcg ccgacagccg cgggatcgac ccggtcgccc agatcacggg cgagcgcatg
      961 tacaacttcg acgtcagcaa cggccagcag gtgctgttca ccggctgcca cgcgagcaag
     1021 ggccggcatc acttcgtctc caacggcacg acctggacct cgggcgtggt gttccaccgc
     1081 tcgacctcga ccggcgccta cgcgatgagc gagggtcacc ggcagtggag cacgggcatg
     1141 ctgtacgaca acatcatcga gagcgacctc aacaaggacg gcatgacggc gctcgcgctc
     1201 tacaaccacg gcgactacgg caccagccac ggctggggca acgcgcactc ggtcgcgtgg
     1261 aactacgacg tcagcaccgg ccgcggcctg atccagcgcc caccgaccgc gcaaaactac
     1321 gcgatcggcg gcgccggcac cttcgccggc acgaagccgc cggccccctt cgaccacccc
     1381 gagggtcaca tcgagggcat cggcaagccc ggcctcgtgc ccgagtcgct gtacgagcgc
     1441 cagctcgccg agcgcctctg cggcctgcgc tgatgtgaac gcagggcgct cctcgtacgt
     1501 ctgcggtcgc cgggaggcga ccgcgtccgc gtagc
//
LOCUS       DASWNJ010001079         1788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_55684, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001079 DASWNJ010000000
VERSION     DASWNJ010001079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55684"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VGB85_18180"
     CDS             complement(<1..619)
                     /locus_tag="VGB85_18180"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY0136023.1"
                     /translation="MTMVSGALADAAPAAALDLKGALQHMVREGASDLHLKVGRPPTL
                     RLHGDLVPLAMPAMRPEELRTLAEQLLPPAQLREFVEMREADFAINVPGIGRFRVNVY
                     QQKGTVAFAMRSIAHAAASIRDLNLPPVLEQIALTPRGLVLVTGVTGSGKSTALAAMV
                     QHINDRRAANIITIEDPIEYVHRDVQSHINQREVGTDTATFAQALR"
     gene            complement(631..1131)
                     /gene="accB"
                     /locus_tag="VGB85_18185"
     CDS             complement(631..1131)
                     /gene="accB"
                     /locus_tag="VGB85_18185"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEY0136024.1"
                     /translation="MIDLRYVTKLIEMLDGSTVDSIEISSDKGMKLRISKTPQQRAAM
                     QVAAPVPMPAMLAAPTAPAQPTPAESMRAVAEAAAAPAAPSKLLEVKSPMVGTFYASA
                     EPGAKAFVAVGQRVAKGQVVCIIEAMKIMNELESEYDGVVREVNVADSHPVEYGQALF
                     RIDPTG"
     gene            complement(1215..>1788)
                     /locus_tag="VGB85_18190"
     CDS             complement(1215..>1788)
                     /locus_tag="VGB85_18190"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEY0136025.1"
                     /translation="GALERALRDEGSEGFPFPSIVASGPRSALPHARSSPRVVGANEL
                     LLMDFGAEVGGYCADVTRTFVVGTADARQREVYAVVQGANALASERVRAGMRGRDADA
                     LARDYIDGLGYGDAFGHSLGHGLGLEVHEAPRLAKSAEAALPTDSVVTIEPGIYLPAW
                     GGVRIEDDVYLGAAGPTVLTHFTRDLVELS"
BASE COUNT          250 a          630 c          637 g          271 t
ORIGIN      
        1 gccggagcgc ctgcgcgaac gtcgccgtgt cggtgccgac ctcgcgctgg ttgatgtggc
       61 tctggacgtc gcggtgcaca tactcgatcg ggtcctcgat cgtgatgatg ttggcggcgc
      121 gccggtcgtt gatgtgctgg accatcgccg ccagcgccgt gctcttgccc gacccggtca
      181 cgcccgtcac cagcacgagc ccacgcggcg tgagcgcgat ctgctccagc actggcggca
      241 ggttcaggtc gcggatcgac gcggcggcgt gcgcgatgga acgcatcgcg aaggcgacgg
      301 tgcccttctg ctggtagacg ttgacgcgaa accgcccgat gcccggcacg ttgatcgcga
      361 agtcggcctc gcgcatctcg acgaactcgc ggagctgcgc gggcggcagg agctgctcgg
      421 cgagcgtgcg cagctcctcg gggcgcatcg cgggcatcgc gagcgggacg aggtctccat
      481 ggaggcgcag cgtcggtggg cgccccacct tgagatgcag atccgacgcg ccttcgcgca
      541 ccatgtgctg cagggcgccc ttgaggtcga gcgccgcggc gggcgcggcg tcggcaaggg
      601 cgccggagac catcgtcatg tcgtcatgtc tcagccggtc ggatcgatgc ggaagagcgc
      661 ctgtccatat tcgacgggat gcgagtcggc gacgttcacc tcgcgcacca caccgtcgta
      721 ctcggactcg agctcgttca tgatcttcat cgcctcgatg atgcagacca cctgcccctt
      781 cgcgacgcgc tggccgacgg cgacgaacgc cttggcgccc ggctcggccg aggcgtagaa
      841 ggtgccgacc atcggcgact tgacctcgag cagcttgctc ggtgccgccg gcgcggccgc
      901 cgcctccgcg accgcccgca tgctctccgc cggcgtcggc tgcgcgggcg ccgtgggggc
      961 ggccagcatc gccggcatcg gcaccggcgc ggccacctgc atcgcggcgc gctgctgcgg
     1021 cgtcttcgag atgcggagct tcatcccctt gtcggaggag atctcgatgg agtcgacggt
     1081 cgacccgtcg agcatctcga tgagctttgt gacgtagcgg aggtcgatca tagggtcggt
     1141 aagggagtcg ccgcgtggag cgggcacgtc gcgccgggca tccggtcgcg tggtctcggg
     1201 cgcacgtggg ctggtcacga gagttcgacg agatcgcgcg tgaagtgcgt gagaacggtg
     1261 ggtcctgccg cgccgagata cacgtcgtcc tcgatgcgca cgccgcccca cgctgggaga
     1321 tagataccag gttcgatggt gaccacggaa tcggtgggca gcgccgcctc ggccgacttg
     1381 gccagccgcg gcgcctcgtg cacctcgagg ccgagcccgt gtccgaggct gtgtccgaac
     1441 gcatccccgt acccgagccc gtcaatgtag tcgcgggcca gcgcgtcagc atcccggccg
     1501 cgcattccgg cccgcacccg ctcggaggcc agcgcgttcg ccccctgcac gaccgcgtag
     1561 acctcgcgct gccgcgcgtc ggccgtaccc acgacgaacg tgcgcgtcac gtcggcgcag
     1621 tagccgccca cctcggcgcc gaagtccatg agcagcaact cgttggcgcc gaccacgcgc
     1681 ggcgacgacc gcgcgtgcgg cagcgccgac cgcggccccg aggccacgat ggacgggaac
     1741 gggaacccct cgcttccctc gtcgcggagc gcgcgctcga gcgcgccg
//
LOCUS       DASWNJ010001080         3952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_616204, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001080 DASWNJ010000000
VERSION     DASWNJ010001080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616204"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1456)
                     /locus_tag="VGB85_18195"
     CDS             complement(<1..1456)
                     /locus_tag="VGB85_18195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136026.1"
                     /translation="MRAPVVLGALALILLHAFPYAERTRNANERPRLLQGMALVEEGT
                     LAIDGLIVRGIERGPDIARSPATGRVYPNKPPGTSLVAAVAYATAREVARASAEPLTL
                     RGYTWWARLFGGVLPTLVLAAALGRRFGPVLGARPVWLAIGLLVLATPMAAYAHLLYG
                     HALTACLLWVGAVVVVDACRDDRPGYAAWGGALAGAAVGVEYSAAFAGVPLAVFVLVW
                     ARRHWRASLGATLGAAGSLALLAWYHAAAYGSPLRTGYHHVTDPGFAAKHGEGLLGLV
                     APSWTALHAHVLSHASGLLWWAPLVLLALAGLWDMSRGPADSAVRSHARVHLAMFLAL
                     LLACISLNFEGGWRVGPRYLVAVLPALVLGWAGAVRWVHAGRAGGWVAFVALLTWSTV
                     LNGLAASLWPHFDLGNIHQPVSEVLLPLWKAGRAPYVAGGIGVTAVIAASVLGLLGLL
                     GSRGRIAATVVGVGLGVGLVAATKLIPPHPRARAN"
     gene            complement(1597..2301)
                     /locus_tag="VGB85_18200"
     CDS             complement(1597..2301)
                     /locus_tag="VGB85_18200"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEY0136027.1"
                     /translation="MTNKPGLFSRLKQVISSTLNDAVDAVTDPGQEIALMLDDLADNI
                     RKSELDLKQAIVDQKMMERKLEQLRKDDASWATRAEQAVNLGDDTLARAALQRKAELA
                     IEVSTLDESVVAQRKLVEDLRAGINQSKTKLKSLNLRRGSLMAQARASKQSGGVSNIP
                     GDAGAGARMDAIEDKIARIEALNEVAAEGAEQQLQEAAIDAKLAQLDKKGVGQDDALE
                     ALKAKMRAKALEEGKP"
     gene            2388..3557
                     /locus_tag="VGB85_18205"
     CDS             2388..3557
                     /locus_tag="VGB85_18205"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136028.1"
                     /translation="MPAPAPTPASAAPAGFTGFPGVRGHREAQEALARALGRGQMHHA
                     LVLVGPRGVGKATFARGLACGLLCPERPGEGCGRCTTCQRVLAGNHPDVDWLLPDGKA
                     GTISVEAARACHVRQAHAPYEGKAYVVVVDPADALGEGAGNALLKTIEEPRPGIHFLL
                     LTTNLQGMLPTILSRALPIRVGRLDDTIVGEILQARAPDLPPDRRDMSVLLAEGSAGV
                     AVELASDPSLERCVELLRQALSAVQHGPRAIFSGDQSPLWTAWSAAVGAGEAEPPPEP
                     EPEPPPAPTATGKKKVAAKAKKPEGEAKKKDAPAAQRAAVRRLTELWILHLREQIRGR
                     AGLTGVSPGPRTANPAQLVATIAALQQLEARVERMGNVRLTLEQTLLDLTRPAST"
     gene            3575..3949
                     /locus_tag="VGB85_18210"
     CDS             3575..3949
                     /locus_tag="VGB85_18210"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HEY0136029.1"
                     /translation="MSPPRSFQEFWPYYLGEHRQPLNRAMHYVGTFCALCLLGYIITN
                     RYWGLMLLGLLVGYGPAWVGHFFIENNKPASFKYPLWSFMADVKMLVLKLGGQMDGEM
                     TRLYGSSNPPPDAPLRRGGPTS"
BASE COUNT          542 a         1612 c         1288 g          510 t
ORIGIN      
        1 ggttggcccg cgcgcgcgga tgcggcggga tcagcttcgt cgccgcgacg agcccgaccc
       61 cgagcccgac cccgacgacc gtcgcggcga tccgcccccg cgagccgagc aggccgagca
      121 ggccgagcac gctcgccgcg atcacggccg tcaccccgat accaccggcg acgtagggcg
      181 cgcgcccggc cttccacagc ggcagcagca cctccgacac cggctggtgg atgttgccga
      241 gatcgaagtg cggccacagc gacgccgcca gcccattgag caccgtcgac caggtcagca
      301 gcgccacgaa cgcgacccac ccgcccgcgc gtccggcatg cacccagcgc acggctcccg
      361 cccagccgag caccagcgcc ggcaacaccg cgacgaggta gcgcggcccg acgcgccagc
      421 cgccctcgaa gttgaggctg atacacgcga gcaacagcgc gaggaacatc gccaggtgca
      481 cgcgcgcgtg gctgcgcacc gccgagtcgg ctggcccccg cgacatgtcc caaaggccag
      541 ccagcgccag caggaccagc ggcgcccacc acagcaggcc cgacgcgtgc gacagcacgt
      601 gcgcgtgcag ggccgtccag ctcggcgcga ccagcccgag cagcccctcg ccgtgcttgg
      661 ccgcgaagcc ggggtcggtg acgtggtgat agccggtgcg cagcgggctc ccgtatgccg
      721 ccgcgtggta ccaggcgagc agcgcgagcg agcccgcggc gccgagcgtc gcgccgaggc
      781 tcgcccgcca gtgccgtcgc gcccacacga gcacgaacac cgccagcggc acgcccgcga
      841 acgcggcgct gtactcgacg ccgaccgccg cccccgcgag cgctccgccc cacgcggcgt
      901 accccggccg atcgtcgcgg cacgcgtcga cgacgacgac cgccccgacc cacagcaggc
      961 aggccgtcag cgcgtggcca tacaacaggt gcgcgtacgc ggccatcggc gtcgccagca
     1021 cgagcagccc gatcgccagc cacaccggcc gcgccccgag cacgggcccg aagcgccggc
     1081 ccagcgccgc ggcgagcacc agcgtcggca gcaccccgcc gaacagccgc gcccaccagg
     1141 tgtacccgcg cagcgtcagc ggctcggcgc tcgcgcgcgc gacctcccgc gccgtcgcat
     1201 acgcgaccgc cgcgacgagg ctcgtccccg gcggcttgtt gggatagacc cgccccgtcg
     1261 ccggcgagcg agcgatgtcc ggcccgcgct cgatcccccg cacgatcagg ccgtcgatcg
     1321 ccagcgtccc ctcctcgacc agcgccatgc cctgcagcag ccgcggccgc tcgttggcgt
     1381 tgcgcgtccg ctcggcgtag ggaaacgcgt gaagcaagat cagcgcgagc gccccgagca
     1441 cgaccggcgc tcgcacccac ctgtccccgg ggccagcctc gacgaggctg tcccgagagc
     1501 cagcctgcga gccgcccccc ggttccacct gcgacctgtc cccggggcca gccgacctgt
     1561 cctccgggcc ggcgccctgc gggtccggca cggcgcttac ggcttgcctt cctcgagcgc
     1621 cttggcgcgc atcttggcct tgagggcctc gagcgcgtcg tcctggccga cgcccttctt
     1681 gtcgagctgc gcgagcttgg cgtcgatggc ggcctcctgg agctgctgct cggcgccctc
     1741 ggccgcgacc tcgttgagcg cctcgatcct tgcgatcttg tcctcgatcg cgtccatgcg
     1801 cgcgccggcg ccggcgtccc cggggatgtt gctgacgccg ccgctctgct tgctcgcccg
     1861 cgcctgcgcc atcaggctgc cgcggcgcag gttcaaggac ttgagcttgg tcttcgactg
     1921 gttgatcccg gcgcgcaggt cctcgaccag cttgcgctgg gcgaccaccg actcgtcgag
     1981 cgtgctgacc tcgatcgcca gctccgcctt gcgctgcagg gccgcccgcg cgagcgtgtc
     2041 gtcgccgagg ttgaccgcct gctcggcgcg cgtcgcccac gacgcgtcgt ccttgcgcag
     2101 ctgctcgagc ttgcgctcca tcatcttctg gtcgacgatc gcctgcttga ggtcgagctc
     2161 ggacttgcgg atgttgtccg ccaggtcgtc gagcatcagc gcgatctcct gaccggggtc
     2221 cgtgaccgcg tcgaccgcgt cgttgagcgt gctggagatg acctgcttga gccggctaaa
     2281 gagccccggt ttgttcgtca tgtcgcgtcc ttactgaccg cggccagtct acaccacgtg
     2341 tcgccgaact tgccgcgctc gcccgcccgc gcctaagctt tgcccccgtg cccgcccccg
     2401 cgcccacccc cgcctccgcc gccccggccg gcttcactgg ctttccgggg gtccgcggcc
     2461 accgcgaggc ccaggaggcg ctcgcgcggg cgctcggccg cggccagatg caccatgccc
     2521 tcgtgctcgt cggcccgcgt ggcgtcggca aggcgacctt cgcccgcggc ctcgcctgcg
     2581 gcctgctgtg ccccgagcgc cccggcgagg gctgcggcag gtgcaccacc tgccagcgcg
     2641 tgctcgccgg caaccacccg gatgtcgact ggctgctgcc cgacggcaag gccggcacga
     2701 tctccgtcga ggccgcgcgc gcctgccacg tgcgccaggc ccacgccccc tacgagggca
     2761 aggcctacgt cgtcgtcgtc gacccggccg acgcgctcgg cgagggcgcc ggcaacgccc
     2821 tgctcaagac gatcgaggag ccgcgccccg ggatccactt cctgctgctg acgaccaact
     2881 tgcagggcat gttgccgacg atcctcagcc gcgcgctgcc gatccgcgtc ggtcgcctcg
     2941 acgacacgat cgtcggcgag atcctgcaag cccgcgcccc cgacctcccg cccgaccgcc
     3001 gtgacatgtc cgtactgctc gccgagggca gcgccggcgt ggccgtcgag ctcgccagcg
     3061 acccgagcct cgagcgctgc gtcgagctgc tgcgtcaggc cctctccgcc gtccagcacg
     3121 gcccgcgggc gatcttcagc ggggaccagt cgccgctgtg gaccgcctgg agcgcggccg
     3181 tcggcgccgg cgaggccgag cccccgcccg agcccgagcc cgagcccccg cccgcaccga
     3241 ccgcgaccgg caaaaagaag gtcgccgcga aggccaagaa gcccgagggc gaggccaaga
     3301 aaaaggacgc ccctgcggcc cagcgcgccg ccgtgcgccg cctgaccgag ctgtggatct
     3361 tgcacctgcg cgagcagatc cgcggccgcg ccggcctcac cggggtatct ccgggccccc
     3421 gcacggcgaa tcctgcgcag ctcgtcgcca cgatcgccgc cttgcagcag ctcgaggccc
     3481 gggtcgagcg catgggcaac gtccgcctca ccctcgaaca gacgctcctg gacctcaccc
     3541 ggcccgcctc cacgtaaccg ttatactcgc cgccatgtca cccccccgca gcttccagga
     3601 gttctggccg tactacctcg gcgagcaccg ccaaccgctc aaccgcgcca tgcactacgt
     3661 cggcaccttc tgcgcgctgt gcctgctcgg ctacatcatc acgaaccgct actggggcct
     3721 gatgctgctc ggcctgctgg tcggctacgg cccggcgtgg gtcggccact tcttcatcga
     3781 gaacaacaag cccgcgagtt tcaaataccc gctgtggtcg ttcatggccg acgtcaagat
     3841 gctcgtgctc aagctcggcg ggcagatgga cggcgagatg acgcgcctct acggcagcag
     3901 caacccgccc cccgacgcgc ccctgcgccg cggcggaccc acctcttgag cg
//
LOCUS       DASWNJ010001081         3154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_159309, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001081 DASWNJ010000000
VERSION     DASWNJ010001081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159309"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VGB85_18215"
     CDS             <1..259
                     /locus_tag="VGB85_18215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136030.1"
                     /translation="EALKDAVAGFEAAATNSRTVLLQQRLAGARLARGTQILASTTEP
                     VLQAEADALLDAAEQFYRSSGPGYAWRLPDLNGLRRRTPSP"
     gene            376..1362
                     /locus_tag="VGB85_18220"
     CDS             376..1362
                     /locus_tag="VGB85_18220"
                     /inference="COORDINATES: protein motif:HMM:NF039450.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADYC domain-containing protein"
                     /protein_id="HEY0136031.1"
                     /translation="MTSMNLQKLLLGTLGLMFTGCDVPDEEVTERGYNVSSGECPPWE
                     CGYNSAEVNGRAIHELNLDGLANSDGMRIIGFVAPAGILGSYKLDVQGDELVAVGKFG
                     TLRGYQLLGATILVKPVGLLKPAVPIIVAGYSEQKSWAVGGAPVPSYALVYPDVDALL
                     GQRNVCVGDLKDTLATSAVVLGGETYDLESKTVVPNRDRWLTIACAGSAAAKMRLMNY
                     GPQSDFDGEGNSASVAQRQATLKMLTADYCGNGTSYTANGTRLAWENADGTVATSTPL
                     GPLEAVWTSKGALCLEATRLADADVACGLPACDKIKDPLAVGEWLTHVPMTK"
     gene            complement(1416..2429)
                     /locus_tag="VGB85_18225"
     CDS             complement(1416..2429)
                     /locus_tag="VGB85_18225"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0136032.1"
                     /translation="MTAIKDPLRRVGGMLVAPRATVADLSESTGRYDGVWLALAWLAA
                     VGLVDLGDALADLRALGGLGGALGLVQGLWPLLPWLLASTAVEWLLGASRAHRAALCL
                     VPMLVIGGAAHLLAAVVAVPAYVPAALGAAAALALAAYVRPAIATKDMSPGTGRPRLA
                     MIDMIAGALVTALVLVSAGRDLTRLVRAWPTLAPASAGDPLPVFTAPLLDGGVLRSAD
                     LPGRPHLLIFWTTWCGVCRSEMPMYRELAARHPGVQVIAVNADRDGDAAAVRAYRDTH
                     ALPFPIALDDGTMTRGFRVRMYPHLVLVDGQGQLRGVYQGRSFASTLDAAIMRLTQKG
                     AGP"
     gene            complement(2426..>3154)
                     /locus_tag="VGB85_18230"
     CDS             complement(2426..>3154)
                     /locus_tag="VGB85_18230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136033.1"
                     /translation="CDRSTAHAVLELGAREGIASLAELRRRALAGAVLSDSNEAHARA
                     TAWMLRSGPNEPFPDLGAVTGPPAGLSLRELPSDAAPAVAAPAVSRRLHLPGLGRGGA
                     SLALDHLDDLDHLDDLDELDDLDLPIGDDWDDEQDPALALPPAPRRPLAPGLLSTPVG
                     APPPALLRPPVARVAAQKPAAGSVAALPYAAQVRLVLDPGTPLPLAMSLLHRLRAPEL
                     RRVATGKRLAPALVSAARRRTGAA"
BASE COUNT          484 a         1134 c         1142 g          394 t
ORIGIN      
        1 tgaggccctg aaggacgcgg tcgccggctt cgaagctgcc gcaacgaact cgcggacggt
       61 gcttctacag caaaggctcg cgggtgcccg actggcgcgg gggacgcaga ttttggcgag
      121 caccaccgag cccgttttgc aagcggaggc ggacgcgctt ttggacgccg cagagcagtt
      181 ttaccggagt tctgggccgg gttacgcgtg gcgtttgcca gatctgaatg gtttgcgccg
      241 ccgcaccccc tcgccctgac acctacatcg ccccccacat tccttcgaag ggccctaacc
      301 agcggttgca cggccgctgc aggcccgtca aacgtgaagc atcggacgcg tcaagtcgac
      361 gcagaggtga acgccatgac ttccatgaac ctgcaaaagc tccttctcgg caccctcggg
      421 ctcatgttca ccggctgtga tgtcccggac gaagaggtca ccgagcgcgg ctacaacgtt
      481 tcgagcggcg agtgtccgcc ctgggagtgc ggctacaact ccgccgaggt caacggtcga
      541 gcgatccacg agctcaacct cgacgggtta gcgaactcgg acggtatgcg gatcatcggc
      601 ttcgtcgcgc cggcggggat cctcggcagc tacaagctcg acgtgcaggg cgacgagctc
      661 gtcgcggtcg gcaagttcgg gaccctgcgc gggtaccagc tgctcggcgc cacgatcctg
      721 gtcaagccgg tcgggctgct caagccggcg gtcccgatca tcgtcgccgg ttacagcgag
      781 cagaagtcgt gggctgtcgg cggcgcgccg gtccccagct acgcgctcgt gtaccccgac
      841 gtcgacgcgc tgctcggcca gcgcaacgta tgtgtcggcg atctgaagga caccctggcc
      901 acctcggccg tcgtcctcgg cggcgagacc tacgacctcg agagcaagac cgtcgtcccg
      961 aaccgcgacc gctggctgac gatcgcctgc gccggctcgg ccgccgccaa gatgcgcctg
     1021 atgaactacg ggccgcagtc cgacttcgac ggcgagggca actccgccag cgtcgcccag
     1081 cgccaggcga ccctcaagat gctgaccgcc gactactgcg gcaacggcac cagctacacc
     1141 gccaacggca cccggctggc gtgggagaac gccgacggca ccgtggcgac ctcgaccccg
     1201 ctcggcccgc tcgaggcggt gtggacctcc aagggcgcgc tgtgcctcga ggccacgcgc
     1261 ctggccgacg ccgatgtcgc ctgcggcctg cccgcctgcg acaagatcaa ggacccgctc
     1321 gccgtcggcg agtggctcac ccacgtgccg atgacaaagt gagtcgaagt gagtcgaagt
     1381 gaaccgtcgc tcgacgggtc ggaagggcgc cctcctcacg gaccggcgcc cttctgcgtc
     1441 aagcgcatga tggcggcgtc gagcgtgctg gcgaagctgc ggccctgata cacgccgcgc
     1501 agctggccct gaccgtcgac gagcaccagg tgcgggtaca tgcgcacgcg aaagcctcgc
     1561 gtcatcgtgc cgtcgtcgag ggcgatcggg aacggcagcg cgtgggtgtc tcgataggcg
     1621 cgcaccgccg ccgcgtcgcc gtcacgatcg gcgttgaccg cgatgacctg cacgccgggg
     1681 tgacgcgcag cgagctcgcg gtacatcggc atctccgacc tgcacacgcc gcaccacgtg
     1741 gtccaaaaga tcagcaggtg cgggcgaccc ggcaggtccg cgctgcgcag gaccccgccg
     1801 tcgagcagcg gcgccgtgaa caccggtagt ggatctccgg cgctcgccgg cgccagcgtc
     1861 ggccacgccc gtaccaggcg cgtgaggtcg cgtccggcgc tgacgagcac cagcgcagtg
     1921 acgagcgcgc cggcgatcat gtcgatcatg gcgagcctcg gacgacctgt cccgggggac
     1981 atgtccttgg tggcgatcgc ggggcggacg tatgccgcga gcgcgagcgc ggccgcggcc
     2041 ccgagcgcgg ccggcacgta cgcgggcacg gcgacgaccg ccgcgagcag gtgcgccgcc
     2101 ccaccgatca ccagcatcgg cacgagacac agcgccgcgc ggtgggcccg cgaggcgccg
     2161 agcaaccact ccaccgcggt gctggcgagc agccagggca gcagcggcca caggccctgc
     2221 acgagcccga gcgcgccgcc gagacctccg agcgcgcgca ggtccgcgag cgcgtcgccg
     2281 aggtcgacca gcccgaccgc cgccagccac gcgagcgcca gccagacgcc gtcgtaccga
     2341 cctgtacttt cggacaggtc ggcgacggtc gctcgcggcg cgacgagcat gccgccgacg
     2401 cggcgcagcg ggtcctttat cgcggtcacg cggcccccgt gcggcgcctg gccgccgaga
     2461 ccagcgcggg tgcgaggcgc ttgccggttg cgacgcggcg cagctccggg gcgcgcaggc
     2521 ggtgcagcag gctcatcgcc agcggcagcg gcgtgcccgg gtcgagcacg agccggacct
     2581 gcgccgcgta gggcagcgcg gcgacagagc cggccgcggg cttttgcgcg gcgacgcggg
     2641 cgaccggcgg acgcagcagc gccggcggcg gcgcaccgac cggcgtcgac agcagcccgg
     2701 gcgcgagcgg acggcgcggc gcaggcggca gcgcgagcgc cgggtcctgc tcgtcgtccc
     2761 agtcgtcacc gatcggcagg tcgaggtcgt cgagctcgtc gaggtcatcg aggtggtcga
     2821 ggtcatcgag gtggtcgagc gcgagcgaag cgccgccgcg cccgaggccg gggagatgca
     2881 ggcgtcgcga cacggccggc gccgcgacgg ccggcgccgc gtcgctcggc agctcgcgca
     2941 agctcaggcc cgccggcggg cccgtcacgg cgccgaggtc cgggaacggc tcgttggggc
     3001 ccgagcgcag catccacgcg gtcgcccgcg cgtgcgcctc gttgctgtcc gaaaggacag
     3061 ctccggcgag ggcgcgccgg cgcagctcgg cgaggctggc gatgccctcg cgggcgccga
     3121 gctcgagcac cgcgtgggcg gtcgagcgat cgca
//
LOCUS       DASWNJ010001082         2424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_55749, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001082 DASWNJ010000000
VERSION     DASWNJ010001082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55749"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2345
                     /locus_tag="VGB85_18235"
     CDS             <1..2345
                     /locus_tag="VGB85_18235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0136034.1"
                     /translation="RSAAILDRFAGLAHAGTAPPGVADRDLLAQIPTLLAEIAAGLVD
                     DERPAPLQAPPERVELAAIVHAHDLLHGCVLDLLEEATIVPGPGELRRLAGLLAAATG
                     HAVRHRSDEHRDGAEDERRHLLGLFQQAPGFVCFLRGPELVFELANAAYYQLVGHRDI
                     LGKPVRAALPEVEGQGYFELLGRVLSEGEPFIGRGMRVLLQREPGAAPSEAFIDLIYQ
                     PIRDHDGRVSGILAQGHDVTEVKRQEAQRQLAEAALRDSEARYRTLFESIDDGFCLIQ
                     VIFDERGDPVDYCFIETNAAFENQTGLRDAIGKTIRELVPDLDESWFRLYGRVATTGE
                     STRFENHAPAMGRWFEVYASRVGAPERRLVALVFKDVSERRRVEEERGRLFELESEAR
                     RKAEDASRLRDEFLTTVSHELRTPLTAILGWVQMLRAGSVPPERRERALETVERNARA
                     QAQLIEDLLDVSSILAGKMRLEVESVEVRAVVEAALETVRPSAEAKHVHLQATLAPAA
                     AVMADCQRMQQVVWNLLANAVKFTPAGGHVHVLVERHEVDVEITVADTGKGIPRDFQP
                     HVFERFRQADGATTRAHGGLGLGLSIVRQLVEMHGGTVSVFSEGEGRGATFTVRLPLA
                     APRPRPARVAPGRDPHGPGLPAPPELAGLHVLVVEDEPDTGEMLRNLLEQCGLEVTLA
                     DSVATALDRFAERRPDLLLSDIGMPGEDGHVLIQRIRRLPAGHGGDLPAVALTAYARA
                     EDRTRALLAGFDNHVAKPVDPGELLAVLASLATRPASRRA"
BASE COUNT          330 a          972 c          809 g          313 t
ORIGIN      
        1 cccggagcgc cgcgatcctc gatcgcttcg ccgggctggc gcacgccggg accgccccac
       61 ccggcgtcgc cgaccgcgac ctgctcgcgc agatccccac gctgctcgca gagatcgccg
      121 ccggcctcgt cgacgacgag cgacccgcgc cgctccaggc accgccggag cgcgtcgagc
      181 tcgcggcgat cgtccatgcc cacgacctcc tgcacggctg cgtgctcgac ctgctcgagg
      241 aggcgacgat cgtccccggc cccggcgagc tgcgccgcct cgccgggcta ctggccgcgg
      301 cgaccggcca cgccgtgcgc caccgatccg acgagcaccg cgacggcgcc gaggacgagc
      361 gccggcacct gctcggcctg ttccagcagg cgcccggctt cgtgtgcttc cttcgcgggc
      421 ccgagctcgt gttcgagctc gccaacgccg cctattatca gctcgtcggc caccgcgaca
      481 tcctcggcaa gccggtgcgc gccgccctgc ccgaggtcga gggtcagggc tacttcgagc
      541 tgctcgggcg cgtgctcagc gagggcgagc ccttcatcgg ccgcggcatg cgggtgctgc
      601 tgcagcgcga gcccggggcc gccccgagcg aggccttcat cgacctcatt tatcagccga
      661 tccgcgacca cgacggccgc gtctccggca tcctcgcgca gggccacgac gtcaccgagg
      721 tcaagcgcca ggaggcccag cgccagctcg ccgaggccgc cctgcgcgac tcggaggccc
      781 gctaccggac cctcttcgag tcgatcgacg acggcttttg cctgatccag gtgatcttcg
      841 acgagcgcgg cgaccccgtc gattactgct tcatcgagac caacgccgcc ttcgagaacc
      901 agaccggcct gcgcgacgcg atcggcaaga cgatccgcga gctggtcccg gacctcgacg
      961 agtcgtggtt tcgcctctac ggccgcgtcg ccaccaccgg cgagtcgacc cgcttcgaga
     1021 accacgcccc cgcgatgggc aggtggttcg aggtctacgc cagccgcgtc ggcgcgcccg
     1081 agcggcgcct cgtcgccctc gtcttcaagg atgtcagcga gcgcaggcgc gtcgaggagg
     1141 agcgcgggcg tctgttcgag ctcgagagcg aggcccggcg caaggccgag gacgccagcc
     1201 gcctgcgcga cgagttcctg acgaccgtca gtcacgagct gcgcaccccg ctgaccgcga
     1261 tcctcggctg ggtgcagatg ctgcgcgccg gcagcgtgcc gcccgagcgc cgcgagcggg
     1321 ccctggagac ggtcgagcgc aacgcccggg cccaggccca gctgatcgag gacctgctcg
     1381 acgtcagcag catcctcgcc ggcaagatgc ggctcgaggt cgagtccgtc gaggtacgcg
     1441 cggtcgtcga ggccgcgctg gagaccgtgc gaccgagcgc cgaggccaag cacgtgcacc
     1501 tgcaagcgac cctcgccccc gcggccgccg tcatggccga ctgtcagcgc atgcagcagg
     1561 tcgtgtggaa cctgctcgcc aacgccgtca agttcacccc cgccggcggc cacgtccacg
     1621 tgctcgtcga gcgccacgag gtcgacgtcg agatcaccgt cgccgacacc ggcaagggca
     1681 tcccccgcga cttccagccg cacgtgttcg agcgcttccg ccaggccgac ggcgcgacca
     1741 cccgcgccca cggcggcctc ggcctcggcc tctcgatcgt gcgtcaactg gtcgagatgc
     1801 acggcggcac cgtcagcgtg tttagcgagg gcgagggccg cggcgcgacc ttcaccgtgc
     1861 gcctgccgct cgccgctccg cgcccgcgac cggcacgcgt cgcccccggc cgggacccgc
     1921 acgggcccgg cctgcccgcc ccgcccgagc tcgccggcct gcacgtgctc gtcgtcgagg
     1981 acgagcccga caccggtgag atgttacgaa acctgctcga gcagtgcggg ctcgaggtca
     2041 cccttgcaga ctcggtcgcc acggcgctgg accgcttcgc cgagcgccgc ccggacctgc
     2101 tgctctccga catcggcatg cccggcgagg acggccacgt gctgatccag cggatccggc
     2161 ggctgccggc cggtcatggc ggcgacctgc ccgcggtcgc cctgaccgcc tacgcccgcg
     2221 ccgaggaccg cacccgtgcc ctgctcgccg gcttcgacaa ccacgtcgcc aagccggtcg
     2281 accccggcga gctgctggcc gtgctcgcca gcctcgccac gcgcccggcc tcgcgccgcg
     2341 cctgaaagcc ccgcgtctcc cggggcgccc ggaaaactcc gaaagatcgt cacggcgcca
     2401 tgtcgatcgc gggccgcccc gttc
//
LOCUS       DASWNJ010001083         2443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_371439, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001083 DASWNJ010000000
VERSION     DASWNJ010001083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371439"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VGB85_18240"
     CDS             <1..237
                     /locus_tag="VGB85_18240"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEY0136035.1"
                     /translation="DLQAGETVLVAAHGNSLRGLVAHLDGLSDEEVVGLNIPTGQPLR
                     YDLDEKMHPTNPGGTYLDESAAKAAAEAVASQGR"
     gene            248..919
                     /locus_tag="VGB85_18245"
     CDS             248..919
                     /locus_tag="VGB85_18245"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEY0136036.1"
                     /translation="MPSKRKKRTGPPPRPAAKPHRPRRTSGPATKAQQQAAAAQRRRQ
                     ARLRRLVLALLLLAAVAAVAAYVVSERREDESLAQALTAGTCDVDTESDTISGGDGHV
                     ASPSYSVDPPAGGDHLSSVARGGVYDGDAVPADGQLVHALEHGYVVAWHAPDLPAEQV
                     EQLAAFEQRHDGDVIVAERDGMTTPVAATAWGQRLLCGEVEPDALDRFAGAHVGNGPE
                     DVPRG"
     gene            complement(1023..2213)
                     /locus_tag="VGB85_18250"
     CDS             complement(1023..2213)
                     /locus_tag="VGB85_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEY0136037.1"
                     /translation="MAGRLRDNAGRAKVWELRARGMVTAQIAEALGTSSSVVNQWVHE
                     RGGVRPTPSRPPSGRYLSLDEREVIAIRLAQGVCNADIAVELGRDRSTIGREVKRNAN
                     PFKSASHRTRPPYLAGRAQRRADTRKPRPKPAKLRADVNAELHDYVQTKLTLTWSPEQ
                     IAERLPVDFPDRPEMRVSPETIYQAIYVQSRGALKRELASCLRTGRAVRRPKRSSTAR
                     RPRVPAELLISQRPAEVEDRAIPGHWEGDLIIGKDNGSAVGTLVERTTRFCMLLHLPD
                     KHGAEQVRDAIITTLSTLPQALRATLTWDQGIELTRHAEITLATDLAIYFCDPHSPWQ
                     RGSNENTNGLLRQYLPKGTDLRQHSAERLAEIAAELNDRPRKTLGWRKPREALNELLV
                     ATTG"
BASE COUNT          336 a          879 c          871 g          357 t
ORIGIN      
        1 gacctgcagg ccggcgagac cgtcctcgtc gccgcccacg gcaactcgct gcgcgggctc
       61 gtcgcccacc tcgacggtct gtccgacgag gaggtcgtcg gcctgaacat cccgaccggc
      121 cagcccctgc gctacgacct cgacgagaag atgcacccga ccaaccccgg cggcacctac
      181 ctcgacgagt cggcggccaa ggccgcggcc gaggccgtcg cgtcgcaggg gcgctgagag
      241 gacccgcgtg cccagcaaga ggaagaagcg gaccggcccg ccgccgcgac cggcagccaa
      301 gccccaccgt ccccggcgca ccagcggccc ggcgaccaag gcccagcagc aggcggccgc
      361 cgcccagcgc aggaggcagg cccggctgcg gcggctcgtt ctcgccctgc tcctgctggc
      421 ggccgtcgct gccgtggcgg cgtacgtcgt cagcgagcgg cgggaggacg agtcgctcgc
      481 gcaggcgctc acggcgggca cctgcgacgt cgacaccgag agcgacacga tcagcggcgg
      541 cgacgggcac gtcgcgtcac cgagctactc ggtggaccca cctgccggcg gtgaccacct
      601 gagctcggtc gctcggggtg gcgtctacga cggcgacgcg gtcccggccg acgggcagct
      661 ggtccacgcc ctcgagcacg gctacgtcgt ggcctggcac gcgcccgacc tgccggccga
      721 gcaggtggag cagctagcgg cgttcgagca gcgccacgac ggcgacgtca tcgtcgccga
      781 gcgcgacggc atgacgacgc ccgtggccgc gacggcctgg gggcagcgcc tgctctgcgg
      841 cgaggtggag cccgacgcgc tcgaccgctt cgccggggcc cacgtcggca acggcccgga
      901 ggacgtcccc cgcggctagg cgcgggcgac ggcggcgcct ggcatgatca ccgcaggacg
      961 cgcacgagct cccgcggcgt cgtcgcgagt ggcacgtcga ccgcacgaca cggcgggtgg
     1021 attcagccgg tcgttgcaac gagcagttcg ttgagtgctt cgcgtggctt tctccaacct
     1081 agggtcttgc gcggtcggtc gttgagctcg gcggcgatct cggcgaggcg ctcggcgctg
     1141 tgctggcgca ggtcggtgcc tttgggcagg tactggcgga gcaggccgtt ggtgttctcg
     1201 ttgctgccgc gttgccaggg gctgtgcggg tcgcagaagt agatcgccag gtcggtggcg
     1261 agggtgatct ccgcgtgccg ggtcagctcg atgccctggt cccaggtcaa cgtcgcgcgc
     1321 agcgcctgcg gcaaggtgct gagcgtggtg atgatcgcgt cgcggacctg ctcggcgccg
     1381 tgcttgtccg gcaggtgcag cagcatgcag aagcgggtcg tgcgctccac gagcgtgccg
     1441 acagcggagc cgttgtcctt gccgatgatc aggtcgcctt cccagtggcc ggggatggca
     1501 cggtcttcga cctcggcggg tcgttggctg atgagcagct cggccgggac ccgcggtcgt
     1561 cgagcggtgc tgctgcgctt gggccggcgg accgcccggc cggtccgcaa gcagctggcg
     1621 agttcacgtt tgagcgcacc gcgggactgc acgtagatcg cctggtagat cgtctcaggg
     1681 ctcacacgca tctccggccg atcggggaag tcgaccggca ggcgctcagc gatctgctcc
     1741 ggtgaccacg tcaacgtcag tttcgtctgc acgtagtcgt gcagctcggc gttgacgtcc
     1801 gcccgcagct ttgccggctt cggccgtggc ttgcgggtgt cggcccggcg ctgcgcccgc
     1861 cccgccaggt acggcgggcg agtgcgatga ctggcgctct tgaacgggtt ggcgttgcgc
     1921 ttgacctccc gcccgatcgt gctgcggtcc cggccgagct ccacagcgat gtcggcgttg
     1981 cacacgcctt gggccagacg gatggcgatg acctcacgct catccaggct caggtaacga
     2041 ccagacggcg gccttgacgg cgtgggccgc actccaccgc gctcatgcac ccactggttg
     2101 acgacgctgg aactggtgcc gagcgcctca gcgatctgcg cagtcaccat ccctcgagca
     2161 cgcagctccc agaccttcgc ccgaccggcg ttgtcccgaa gccgccctgc gatccacacc
     2221 gacatcgacg catcccttga tcatccacgt cagtgttgca acgaccacaa gaacccaagc
     2281 cggcgagtct ccggtgatca tgccgaccgc gccgcgcggg tcggcccggc ccgcgcctac
     2341 tcccgcagca gctcgtgctc caccagggcg ttcggcagca ccgatccgag cagctcctcg
     2401 cgcggcatct gcgcctcgcc gccgaaggcc gtgccgagcg cgt
//
LOCUS       DASWNJ010001084         2268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_655968, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001084 DASWNJ010000000
VERSION     DASWNJ010001084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655968"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2268
                     /locus_tag="VGB85_18255"
     CDS             <1..>2268
                     /locus_tag="VGB85_18255"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0136038.1"
                     /translation="TVDLIGAREREIQVLVDPARMTGFGLTVDDVSGAIQAGNIELPA
                     GYVKTGGNELTIKTRGEVKSAQEIADIVIRGVGGGAIRIKDVAEVVDSVEEARSASFF
                     NGEPALALVVRKQSGANVVALAEQVREVVEEMRPELAKKGIKLEMPLDNSVFAEHAID
                     DVKFDLILGAILTIIIILVFLHDIRATFIAALAIPTSVVATFAFMSYMGFSFNNISML
                     ALSLSIGILVDDAIVVIENIYRHLEMGKPRMRAALDATSEIGLAVIATTLSIVAVFVP
                     VAFMEGMIGRFFFQFGLTVSAAVLLSMLVSFTLTPMLSSRLMRMQHTHAPGLFARSFN
                     RMFLGVENMYAAVLKWALRWPWTTVFLAIGSLVGAVALVTRVPAEFIPLQDQSQFSIS
                     IELPTGTGLESTIATTEAIGKDLRENLVGYKDTFTTIGGGAQGQVNRATTTVTLVNRK
                     QRSFSQHDAMQWARDRYAGTPGVMVTVEEVNLFGGDGGFRTQPVQFSIRGTNLDEVTA
                     AAQALRAELDKTGVFVDLDITYRGGKPEVAIQVDRNKAADLGVPVASVARTVRALMAG
                     DPISDLKEGSDIYDVILQMPEAQRSNIEALSNLKVRAVTGQLVDLASVVTVGRTSGPS
                     EIERQNRLRQIVVLANIKGVTLGEAQEIVNEAAARVVPAHLTTSYLGDAEIMIEAFTA
                     MLLALALAVVLVYMILAAQFDSLIQPITIMISLPLSVVGAFGGIYLAGMTLNIFSFIG
                     LIMLMGLVTKTAILLV"
BASE COUNT          361 a          818 c          710 g          379 t
ORIGIN      
        1 accgtcgacc tgatcggcgc ccgcgagcgc gagatccagg tgctcgtcga cccggcgcgc
       61 atgaccggct tcggcctgac cgtcgacgac gtcagcggcg cgatccaggc cggcaacatc
      121 gagctgcccg ccggctacgt caagaccggc ggcaacgagc tgacgatcaa gacccgcggc
      181 gaggtcaagt ccgcccagga gatcgccgac atcgtcatcc gtggcgtcgg cggcggcgcg
      241 atccgcatca aggacgtcgc cgaggtcgtc gactccgtcg aggaggcccg ctcggcctcc
      301 ttcttcaacg gcgagcccgc gctcgccctc gtcgtccgca agcagagcgg cgccaacgtc
      361 gtcgccctcg ccgagcaggt ccgcgaggtc gtcgaggaga tgcgccccga gctcgccaag
      421 aagggcatca agctcgagat gccgctcgac aactcggtgt tcgccgagca cgcgatcgac
      481 gacgtcaagt tcgacctgat cctcggcgcg atcctgacga tcatcatcat cctggtgttc
      541 ttgcacgaca tccgcgcgac cttcatcgcc gcgctggcga tcccgacctc ggtggtcgcg
      601 accttcgcgt tcatgtcgta catgggcttt agcttcaaca acatctcgat gctggcgctg
      661 tcgctgtcga tcggcatcct cgtcgacgac gcgatcgtcg tgatcgagaa catctatcgc
      721 cacctcgaga tgggcaagcc gcgcatgcgc gccgccctcg acgcgacctc cgagatcggc
      781 ctcgccgtca tcgccaccac cctgtcgatc gtcgccgtgt tcgtgccggt cgccttcatg
      841 gagggcatga tcggccggtt cttcttccag ttcggcctca ccgtctcggc ggcggtcctg
      901 ctgtcgatgc tggtgtcgtt caccctgacc ccgatgctgt cgtcgcgcct gatgcggatg
      961 cagcacacgc acgcgcccgg cctgttcgcg cgcagcttca accgcatgtt cctgggcgtc
     1021 gagaacatgt acgccgcggt cctcaagtgg gcgctgcgct ggccgtggac gaccgtgttc
     1081 ctggcgatcg gctcgctcgt cggcgccgtc gcgctcgtca cccgcgtgcc cgccgagttc
     1141 atcccgctgc aggaccagtc gcagttctcg atctcgatcg agctgccgac cggcaccggc
     1201 ctcgaatcca cgatcgccac caccgaggcg atcggcaagg acctgcgcga gaacctcgtc
     1261 ggttacaagg acacctttac gacgatcggc ggcggcgccc agggccaggt caaccgcgcg
     1321 acgaccactg tcaccctcgt caaccgcaag cagcgcagct tctcgcagca cgacgcgatg
     1381 cagtgggcgc gcgaccgcta cgcgggcacg cccggcgtga tggtcacggt cgaggaggtc
     1441 aacctcttcg gcggcgacgg cggctttcgc acgcagccgg tgcagttttc tatccgaggc
     1501 accaatctcg acgaggtcac ggcggcggcc caggccctgc gcgccgagct cgacaagacc
     1561 ggcgtgttcg tcgacctcga catcacctac cgcggcggca agccggaggt ggcgatccag
     1621 gtcgaccgca acaaggccgc cgacctcggc gtgccggtcg cctcggtcgc ccgcacggtc
     1681 cgcgcgctga tggccggtga tccgatctcc gacctcaagg agggctccga catctacgac
     1741 gtgatcctgc agatgccgga ggcgcagcgc tccaacatcg aggcgctgag caacctcaag
     1801 gtgcgcgcgg tgaccggcca gctggtcgac ctcgccagcg tcgtcaccgt cggccgcacc
     1861 agcggcccca gcgagatcga gcgacaaaac cgcctgcgcc agatcgtcgt gctcgccaac
     1921 atcaagggcg tcacgctcgg cgaggcgcag gagatcgtca acgaggccgc cgcgcgggtc
     1981 gtgccggccc acctgacgac cagctacctc ggcgacgccg agatcatgat cgaggccttc
     2041 acggcgatgc tgctggcgct cgcgctcgcg gtcgtgctgg tctacatgat cctcgcggcg
     2101 cagttcgaca gcctgatcca gccgatcacg atcatgatct cgctgccgct gtccgtcgtc
     2161 ggcgccttcg gcgggatcta cctcgccggc atgacgctca acatcttctc gttcatcggc
     2221 ctgatcatgt tgatgggcct ggtgaccaag acggcgatct tgctggtc
//
LOCUS       DASWNJ010001085         2572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_371440, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001085 DASWNJ010000000
VERSION     DASWNJ010001085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371440"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /gene="tssJ"
                     /locus_tag="VGB85_18260"
     CDS             <1..523
                     /gene="tssJ"
                     /locus_tag="VGB85_18260"
                     /inference="COORDINATES: protein motif:HMM:TIGR03352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HEY0136039.1"
                     /translation="AHPCTRYGARQSQPQPEATAHTEVRRQLSRLAVAAVAAVTALLA
                     GCHRAPSSTPAPARSAVQVLIQAGPAINPGDDGQPWPTTLTLYQLRGDPDASEPLDPA
                     IVLTRGGAAFGDLLLDRREYTAFPDTRARLVVSLGPEATHLVAVAHFREPLGDAAVLV
                     YPVPHTRAADPCF"
     gene            691..2013
                     /gene="tssK"
                     /locus_tag="VGB85_18265"
     CDS             691..2013
                     /gene="tssK"
                     /locus_tag="VGB85_18265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HEY0136040.1"
                     /translation="MNRPLWTEGTLLCPQHMQQQDLYHEHNLAARLAAALPHPWGITA
                     LRFDPAALQSGQLALTILRAVLPDGTCIELDDRSPQPPPTRQLAPHFAARSERVPVWL
                     TVPVLRPGLVNSAAEPTAQHRYRTVTRDHFDLSLTRSARPLEQSEPNLALRLGDEPRD
                     DLVCLPLAEVVRDPGGSFVLDETFIPPISTVAAAPPLRATLQNLCALVGARRRTLADD
                     RRRHHDLPRALFFHALSGALPLLRHLADSPDTTPLQLYQHLLRLAGELRSFGDPGEPG
                     DPTPYDFADLRACLGPLLSDLRQRVVDTLPDRFLRVPLEPRADGLWIGELRDDRLLRC
                     TSFVLAVEAGADPAVLSSELPALTRIAAWRRINLIVRNNVLGAPIRAVTHPPVELPVL
                     PRHAYFTITPDDPSWLEVLRERNVAIYLPRPYDPEHARITLMAIPDEA"
     gene            2013..>2572
                     /locus_tag="VGB85_18270"
     CDS             2013..>2572
                     /locus_tag="VGB85_18270"
                     /inference="COORDINATES: protein motif:HMM:NF021377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HEY0136041.1"
                     /translation="MSAQTSNERTVAAWVDRRSAPCFHHALLLQRAELRTWDDPTADP
                     ILQSLLKSLAGLQLDDEARELGLHRDHLAAIRFAIVALLDECAAHQPGSERWRERLFD
                     GAGLPRSANVGHEFFVALEQRLAVPAPTPADLAVLQVHAVCLFLGLRGRYGAHVEAAE
                     SELQHQLRRLHLKLRPLLAPRAPPTFP"
BASE COUNT          331 a         1205 c          684 g          352 t
ORIGIN      
        1 agcgcacccg tgcacccgct atggtgccag gcaatcgcag cctcagcccg aggccacggc
       61 gcacacggaa gtgcggcgac aactatctcg cctcgcagtc gccgcggtcg ctgcagtcac
      121 ggcgctgctc gcaggctgcc accgcgcccc ctcctccacg cccgcgccgg cccgcagcgc
      181 cgtccaggtg ctcatccagg ccggtcctgc gatcaacccc ggcgacgacg gccagccctg
      241 gccgaccacg ctgaccctct atcaactgcg cggcgacccc gacgcgtccg agcccctcga
      301 ccctgccatc gtgctcacgc ggggcggcgc cgccttcggt gacctcctgc tcgaccgccg
      361 cgagtacacc gccttccccg acacccgcgc gcgcctcgtc gtctcgctcg gcccggaggc
      421 cacgcacctc gtcgccgtcg cccacttccg cgagccgctc ggtgacgccg ccgtgctcgt
      481 ctaccccgtc ccgcacaccc gcgccgccga cccctgcttt tagctcggcc tcgagcgcag
      541 cgagctcgac ggcggcgagt tcccgccgcc cggcttcgac ccggccgcct tcgccaccac
      601 ctgcccctcg cccgccgacc tcaccccgct ccaactaccc gctccgggct cacctgtccc
      661 tccggacagc ccgctcgcca cgaggtcgcc atgaaccgcc ctctgtggac cgagggcacc
      721 ctgctgtgtc cgcagcacat gcagcagcag gacctctatc acgagcacaa cctcgcggcc
      781 cgcctcgcgg ccgccctgcc gcacccgtgg ggcatcaccg ccctgcgctt cgacccggcc
      841 gccctgcaat ccggccagct cgccctgaca attctgcgcg ccgtcctccc cgacggcacc
      901 tgcatcgagc tcgacgaccg cagcccccag cccccgccga cccgacagct cgccccgcac
      961 ttcgccgctc gcagcgagcg cgtccccgtg tggctcaccg tcccggtgct gcgacctggc
     1021 ctcgtcaaca gcgccgccga gccgaccgcc cagcaccgct atcgcaccgt cacgcgcgac
     1081 cacttcgacc tcagcctcac ccgcagcgcc cgcccgctcg agcagagcga acccaacctc
     1141 gcgctgcgcc tcggcgacga gccccgcgac gacctcgtct gcctccccct cgcggaggtc
     1201 gttcgtgacc ccggcggcag cttcgtcctc gacgagacct tcatcccacc gatcagcacc
     1261 gtcgccgccg caccgcccct gcgcgccact ttacaaaatt tgtgcgccct cgtcggcgcc
     1321 cgccgtcgca ccctcgccga cgaccgccgc cgccaccacg acctcccgcg cgccctgttc
     1381 ttccacgccc tcagcggcgc cctccccctg ctgcgccacc tcgccgacag ccctgacacc
     1441 acccccctcc agctctacca gcacctcctg cgcctcgccg gcgagctgcg cagcttcggc
     1501 gaccccggcg agcccggcga cccgaccccc tacgacttcg ccgacctgcg cgcctgcctc
     1561 ggccccttgc tgtcggacct gcgccagcgc gtcgtcgaca ccctgcccga ccgcttcctc
     1621 cgcgtcccgc tcgagccacg cgccgacggc ctgtggatcg gtgagctgcg cgacgatcgc
     1681 ctgctgcgct gcaccagctt cgtgctcgcc gtcgaggccg gggccgaccc cgccgtgctc
     1741 tccagcgagc tcccggccct gacgcggatc gcagcctggc ggcggatcaa cctcatcgtc
     1801 cgcaacaacg tcctcggcgc accgatccgc gccgtcaccc atccgccggt cgagctcccg
     1861 gtcctgcccc gccacgccta cttcacgatc acccccgacg acccctcgtg gctcgaggtc
     1921 ctgcgcgagc gcaacgtcgc catctacctc cctcgcccct acgaccccga gcacgcccgc
     1981 atcaccctga tggcgatccc cgacgaggcc tgatgtccgc acagaccagc aacgaacgga
     2041 ccgtcgcggc ctgggtcgac cgccgcagcg ccccctgctt tcatcatgcc ctgctgctgc
     2101 agcgcgccga gctgcgcacc tgggacgacc cgaccgccga cccgatcctt caatctctct
     2161 taaaatccct cgccggtctc cagctcgacg acgaggcccg cgagctcggc ctccaccgcg
     2221 atcacctcgc tgcgatccgc ttcgccatcg tcgccctcct cgacgagtgc gccgcccacc
     2281 agcccggcag cgagcgctgg cgcgagcgcc tgttcgacgg cgccggcctc ccgcgcagcg
     2341 ccaacgtcgg ccacgaattc ttcgtcgccc tcgagcaacg cctcgccgtc cccgccccca
     2401 cccccgccga cctcgccgtc ctccaagtcc acgccgtctg cctgttcctc ggcctgcgcg
     2461 gccgctacgg cgcccacgtc gaggccgccg agtccgagct gcaacaccag ctgcgccgcc
     2521 tccacctcaa gctgcgcccg ctgctcgccc cccgcgctcc accgaccttc cc
//
LOCUS       DASWNJ010001086        11113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_550689, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001086 DASWNJ010000000
VERSION     DASWNJ010001086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 11113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11113
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550689"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="VGB85_18275"
     CDS             <1..1010
                     /locus_tag="VGB85_18275"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY0136042.1"
                     /translation="PAGSASGCNGAALTLPKGTVVARIDGKDVTVDDLGGDLERAEKQ
                     ALRTYCQEVTNARQSVLDNLLQQRLLTAAAQAEGKDINSFVQGKVDAEVPTPDDAAIS
                     AFYEANKSPTAPALELVRDQVIQAMKAEGGERVLTKLLDDLRAKAKVERMLPDVSPPP
                     MDLSTPPYAGVAGPEDALVKVVEFSDFECPYCSRAADTMRGLKERYAGKPVQFVYRHF
                     PLSFHPNARPAAEYAQCAQQQGKFWEMHDGIFARQRELSADMLKDVAAKAGLDTAKLE
                     SCLAAGAVGQEVEEDMRKAQEVGVQGTPSFFVNGQTYGGNPTVDGIAQAIDAELARLQ
                     G"
     gene            1023..1976
                     /locus_tag="VGB85_18280"
     CDS             1023..1976
                     /locus_tag="VGB85_18280"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEY0136043.1"
                     /translation="MHPAFLPLPRRFLYALKPASWPKLLVPMVVGQVLGVMSSGTWSW
                     GGAAVGVGFTAGMLCYLVLLNDWFDARVDAIKRQMFPDAGSPKTIPDAILHPQTVLLA
                     GIGFGLCAASIALCGEIVIGRDGLALAGITCLALLLLYSAPPLRLNDRGIGELVEAAG
                     VGVLLPMFNAYAQGGEMVTRELLLVTPGLTALALASAIASGLADEDSDRAGGKATMTT
                     QLGNVAARDAIEYAVMAGAMLWAVAARVGQHQLSPFAFSAALAVLLWQWRGVAAISPR
                     ATTNAFTEIARYKQRLHRALWGSALTLAVALVLLRVLGVDP"
     gene            1973..2719
                     /locus_tag="VGB85_18285"
     CDS             1973..2719
                     /locus_tag="VGB85_18285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136044.1"
                     /translation="MNPALPAEAHARLAEADRSLDVHAWGRRSLAPGREQSRSLLAAC
                     GAVALPDDDAATLVALAAGVAELAQVQRESFPDNLCCDLDLLIADTLRRARASARPVA
                     EVTARLGQVCRLQRLYGGDTAIRFRYVHDFVYGFDWAKWVGRDPDLRAAVGPFDATFL
                     DHLERRAHVLFGLIAADDPVYGQIPEDQTRNPFPFSREPEAELALHRDLAARGLVPVA
                     AWDPDAAPTWRRAFAALRCERAAALGLCAA"
     gene            complement(2721..3341)
                     /locus_tag="VGB85_18290"
     CDS             complement(2721..3341)
                     /locus_tag="VGB85_18290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136045.1"
                     /translation="MTDAAQKSTSSSPGSGTSPAEEPQGSLGAVLLGAAILLIAGLLI
                     FWPGSDTAKNSGAAGGKGGRGDVQAANGVDGDDASGRARGIVPRQADPVAPRPPPPRV
                     RDGLLAPAGGMAIMPTAEPEPTSFPTPAAEIAHLEKKLVEARANLAARTTFLDRMKRI
                     LKEGPISDYERNTARAKIVQENYDKAQSRVDDFEYRIKVLRKQQGG"
     gene            3366..4400
                     /locus_tag="VGB85_18295"
     CDS             3366..4400
                     /locus_tag="VGB85_18295"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY0136046.1"
                     /translation="MDAKDQDMEPQAGDAMIYIPLVVDSARDGFRLDRFLVSRIHRLS
                     RTRVQEIVASGRVRRADTGEVILRPAQRLRVGEALVIERPAPVEPSVVLDYTELHRDA
                     DLLVLDKPAGLPVHPSARYHHHTLTAVMRGRMGRGHGWDMAHRLDRETSGVLIFGRHG
                     GSASVLKRAFSDRTVEKRYWALCLGRVSEPVRIDMPLGPAKGSEIHIKIGPRALDDGG
                     VHAATGVTPLAHGEFRGAPITLIEVRPETGRQHQIRVHMAEIGHPLLGDKLYGLDEAR
                     FLAVVDGERSGSTTPPALALAKLEAELGLGRHALHAVSICFDHPKTGERVEFTAPWPA
                     DLAEIMPAPG"
     gene            complement(4409..5050)
                     /locus_tag="VGB85_18300"
     CDS             complement(4409..5050)
                     /locus_tag="VGB85_18300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136047.1"
                     /translation="MPADREQSDLDSAGLAPTPQLAREHLADPLADPLRDPLSEQPRV
                     RLEGDLAGEERRTTLPPPRERDDELMDNPPVGDYHRGRLGGHQSGRRMRIIFGILAVA
                     LVAGGVGLMVWYVRNRPPQHSSQFELPPGSDLEGRPRVMTWTGGKARLGLDRKPPGVL
                     EISLPDRTLRLADGSDQAQMKVEVEDGKTVALKVIFGEVVEELAPGANPLLAR"
     gene            complement(5319..6812)
                     /locus_tag="VGB85_18305"
     CDS             complement(5319..6812)
                     /locus_tag="VGB85_18305"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0136048.1"
                     /translation="MNAAIKHSTELSDLSPASRYDVVIIGAGIAGCAAAQALALADTS
                     GRRRILLIDRHPGSSPRFAGEFIHPRGAQILDDLGFYGALKAAGAVDVDGFTVLERAD
                     GRYVELPYDDIPEYRTQGLASHHKTLVKTMRQVVRERPQVDLFEGWIITDLVRSGERV
                     AGVVLTGPGNRTVKIHADLVIGADGKASQVRKLAGIPDQRQTVGFTVGLELRNAKLPG
                     RTWGNIILGAWGPVLVYPISHEPNGDLLYRVTLDLPANLPAKGARLAEFLLETFVPFL
                     PQPLADQIAHAITSHKGPFEMAPTVNLPAPTAVQPGLALIGDAGGCSHPITASGMTMG
                     LRDAECLGEEARRREDAPVHEAWVDDSSLRRYRAEHDRYVPTRQALADAIYEVFRGGN
                     EGARAIQRALFEYWASGKTARARSMALLSCAEGRPQVFLTEYLKAARYAIESSLVARH
                     AAHLPTRDRLRQVSGAMHLASNKLGLVAQVMWAQVRPAWLPHDRTFI"
     gene            complement(6857..8767)
                     /locus_tag="VGB85_18310"
     CDS             complement(6857..8767)
                     /locus_tag="VGB85_18310"
                     /inference="COORDINATES: protein motif:HMM:NF024646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEY0136049.1"
                     /translation="MPALSPIRVPTQGRLRAVIARAAAAAAWWHQQTPAQPDRQPHTD
                     GAAPYLLSIPDIGLACEVLLADRLFHDLGTAERESLAGWVRASQQPSGAWHDPAGRPD
                     LSRTALGWWALVESGDDPAAEHLVRARRIVHELGGAQRASIEVRLWMAMAGHIPWSWL
                     PTMPAELWLLPTVTWLSPSRVSPWARGVMTPYLLLSRAPARVHVSNAATLLLTRPDGE
                     PILPRLVQPGLLGDLLQAFDQGVKLIRKLPRGPVLTAALGRVQRWMIAAQQAHGGWFA
                     AHPTLLSLIALRVLGANYDDPRIVRGLAYMRRARGLVHTPAGPQLAQGLTCAPLTTVA
                     RLVRLALASGDPAGEAAVPFFLAEEIRHVGEWQLRTNAPAGGWPCEAGADKYVDTLST
                     CAVLDALHALPKTSPHQSQVRAAMRRAVEVLFAMQEPDGGFARFERGESEVFMTRFPL
                     RDARLLAAGTPTDLARVRVSATALRQLARLGFQADDDRVARGLQWLWQRVTLDMAGFD
                     LATLGELALCTAALCPPTHPLRQTIERQLRGRQREDGGFGSAVDTAVAMQGLMALEGA
                     ACIQTQRAARHLVQLLDNAPPELAQAELSERLGGAWTAGLGLTPMCQDPSAGVREAAL
                     ALTLFAEHGGQI"
     gene            9027..9662
                     /locus_tag="VGB85_18315"
     CDS             9027..9662
                     /locus_tag="VGB85_18315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136050.1"
                     /translation="MNAQARALLMAGCMVGGLSLMGCKEEESERFGFTCIDLNQGEGI
                     KEDPFIGTYRIIVTLNYEPCIKDFYLNKHPEMRQDGKEGPAVFEEWKERLCSEDVDGR
                     IECEVEEFKQTLMSTGTEAYKMSVSYITPKSDQIAGRTLLWGPGPLPEFAECDEGLQP
                     FVNLSALSGVIGEDKDGKTLWQVQSFGDMRGLMQTRGGGCIQANIQPVGGV"
     gene            9713..10312
                     /locus_tag="VGB85_18320"
     CDS             9713..10312
                     /locus_tag="VGB85_18320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136051.1"
                     /translation="MRETASVTEPPSVALAPTCAPPPAAALALIADAPCPWILVSGDR
                     SEMALRATDMVAPRALVVVPPEACGDRCSFTGASTALGPVVLATRHDPASELADAAFI
                     GAALGGGTLRFTPLWYGASAHGDSTDLGPSYALVPWVCEEALVLAIGGRLPGARAEEP
                     ARALAAAAGVYAIADDELRRTDQAMPALTGCTRVPLELP"
     gene            10309..>11113
                     /locus_tag="VGB85_18325"
     CDS             10309..>11113
                     /locus_tag="VGB85_18325"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY0136052.1"
                     /translation="MTEISVPEDMSQEPARDLGLTLAGGGSRSFYQVGLLDRWGEQLR
                     PRLAAVAGVSAGACTGAVWLAGRTEATREYWHWRRRDVHSNFEWRRLLRGERPTPQGP
                     VYRDVLVHAFQEGGLEQLRASEIPMLVMVAVPPRLLHPLAGMLFGYGVYMAEKKIWPA
                     TLHPRAGRKIGFVPRVFDARLCESPEEVAELILASSATPPFTPFGRYRGERYLDGGMV
                     DNVPAFAVEQIAGVRRNLVMMTRPYPARSLGIHGPRLYVAPSGLTPVTCW"
BASE COUNT         1530 a         3728 c         4279 g         1576 t
ORIGIN      
        1 ccccggccgg ctcggcgagc ggctgcaacg gcgccgcgct gacgctgccc aagggcacgg
       61 tcgtcgcccg catcgacggc aaggacgtca ccgtcgacga cctcggcggg gacctcgagc
      121 gcgcggagaa gcaggcgctg cgcacgtact gtcaagaggt gaccaacgcc cgccagagcg
      181 tgctcgacaa cctgctgcag cagcgcctgt tgaccgccgc ggcgcaggcc gagggcaagg
      241 acatcaacag cttcgtgcag ggcaaggtcg acgccgaggt gccgacgccg gacgacgctg
      301 cgatctcggc gttctacgag gccaacaagt ccccgacggc gccggcgctc gagctggtgc
      361 gcgaccaggt gatccaggcg atgaaggccg agggtggcga gcgcgtgctg accaagctgc
      421 tcgatgacct gcgcgccaag gccaaggtcg agcgcatgct gcccgatgtc agcccgccgc
      481 cgatggacct cagcacgccc ccgtacgcgg gcgtcgccgg gcccgaggac gcgctggtca
      541 aggtcgtcga gttctccgac ttcgagtgcc cgtactgctc gcgcgccgcc gacacgatgc
      601 gcggcctcaa ggagcgctac gccggcaagc cggtgcagtt cgtgtatcgc cacttcccgc
      661 tcagcttcca ccccaacgcc cggcccgcgg ccgagtacgc ccagtgcgcg cagcagcagg
      721 gcaagttctg ggagatgcac gacggcatct tcgcgcgcca gcgcgagctg tcggccgaca
      781 tgctcaagga tgtcgcggcg aaggcggggc tcgacacggc gaagctcgag tcgtgtctgg
      841 ccgcgggtgc ggtcggtcag gaggtcgagg aggacatgcg caaggcgcag gaggtcggcg
      901 tgcaggggac gccgagcttc ttcgtcaacg gtcagaccta cggcggcaac ccgacggtcg
      961 atggcatcgc gcaggcgatc gacgccgagc tggcgcggct tcagggctag tcaggagcgg
     1021 cgatgcatcc ggccttctta ccgctgccgc gccggttcct gtacgccctc aagccggcga
     1081 gctggcccaa gctgctggtg ccgatggtgg tcggacaggt cctcggggtc atgtcctcgg
     1141 ggacatggtc gtggggcggc gccgcggtcg gcgtcggctt cacggcgggg atgctctgtt
     1201 atctggtgct gctcaacgac tggttcgacg cgcgcgtcga tgccatcaag cggcagatgt
     1261 tccccgacgc gggctcgccg aagacgatcc ccgacgcgat ccttcacccg cagacggtgc
     1321 tgctcgccgg catcggcttc ggcctgtgcg cggcatcgat cgcgctgtgc ggcgagatcg
     1381 tgatcgggcg cgacgggctc gcgctcgcgg gcatcacctg tctggcgctg ctgctgctgt
     1441 acagcgcgcc gccgctgcgc ctcaatgacc gcgggatcgg cgagctcgtc gaggccgcgg
     1501 gcgtcggcgt gctgctgccg atgttcaacg catatgcgca gggcggcgag atggtcacgc
     1561 gcgagctgct gctggtgacg cccgggctga ccgcgctggc gctggcgtcg gcgatcgcca
     1621 gcgggctggc cgacgaggac agcgaccgcg ccggcggcaa ggcgacgatg acgacgcagc
     1681 tcggcaacgt ggccgcccgc gacgcgatcg agtacgcggt gatggcgggc gcgatgctgt
     1741 gggcggtcgc ggcccgcgtc gggcagcacc agctgtcgcc cttcgccttc agcgcggcgc
     1801 tggcggtgct gctgtggcag tggcgcggcg tggcggcgat cagcccgcgg gcgacgacca
     1861 acgcgttcac ggagatcgcc cgctacaagc agcgcctgca ccgggcgctg tggggtagcg
     1921 cgctgacgct cgcggtcgcg ctcgtgctgc tgcgcgtgct cggggtcgat ccgtgaaccc
     1981 tgccctgccc gccgaggcgc acgcccggct ggccgaggcc gaccgatcgc tcgacgtcca
     2041 cgcctggggc cggcggtcgc tcgcgcccgg acgtgagcag tcgcgctcgt tgttggccgc
     2101 gtgcggcgcg gtggcgctgc cggacgatga cgcggcgacg ctcgtcgcgc tggcggccgg
     2161 cgtcgccgag ctggcgcagg tgcagcgcga gagcttcccg gacaacctgt gctgcgacct
     2221 cgacctgctg atcgccgaca cgctgcggcg cgcccgcgcg agcgcgcgtc cggtcgcgga
     2281 ggtgacggcg cgcctgggcc aggtgtgccg gctgcagcgg ctctacggcg gcgacactgc
     2341 gatccgcttc cggtacgtgc acgacttcgt gtacggcttc gactgggcca agtgggtcgg
     2401 ccgcgaccct gacctgcgcg ccgcggtcgg ccccttcgac gcgacgtttt tggatcacct
     2461 cgagcgccgc gcgcacgtgc tgttcgggct gatcgccgcg gacgacccgg tgtacggcca
     2521 gatccccgag gaccagacgc gcaacccgtt cccgttctcg cgcgagccgg aggccgagct
     2581 ggcgctgcat cgcgacctcg cggcgcgcgg tctggtgccg gtcgccgcgt gggaccccga
     2641 cgccgcgccg acctggcgcc gcgccttcgc tgcgctgcgc tgcgagcgag ccgcggcgct
     2701 cggcctgtgc gccgcctgac tcagccgccc tgctgcttgc gcaggacctt gatccggtac
     2761 tcgaagtcgt cgacgcggct ctgcgccttg tcgtagttct cctgcacgat cttggcgcgc
     2821 gccgtgttgc gctcgtagtc gctgatcggc ccctctttca ggatccgctt catgcggtcg
     2881 aggaaggtgg tgcgcgccgc gaggttggca cgcgcctcga cgagcttctt ctcgagatgg
     2941 gcgatctcgg cggcgggggt cgggaagctc gtcggctccg gctcggccgt cggcatgatc
     3001 gccatgccac cagctggcgc gagcagcccg tcacgaacgc gcggtggcgg cggacggggc
     3061 gcgaccgggt cggcctgccg cggcacgatg ccgcgggcgc gtccgctggc gtcgtcaccg
     3121 tcgacgccgt tggcagcctg gacgtcgccg cgaccgccct tgccaccggc ggcgccgctg
     3181 ttcttggcgg tgtcgctgcc cggccagaag atcagcaggc cggcgatcag caggatcgca
     3241 gcgccgagca gcacggcgcc gagcgacccc tgcggctcct ccgcgggcga ggtgcccgag
     3301 ccaggggacg aggaggtcga cttctgcgcc gcgtcggtca tcgccaggac gttataacat
     3361 gcgacgtgga cgcgaaagac caggacatgg agccgcaggc cggcgacgcc atgatctaca
     3421 tccccctggt cgtcgacagc gcccgcgacg gctttcgcct cgaccgcttt ctggtcagcc
     3481 gcatccaccg gctgtcacgc acccgcgtgc aggagatcgt cgcgtccgga cgggtccgtc
     3541 gcgccgacac cggcgaggtc atccttcgcc ccgcccagcg cctgcgcgtc ggcgaggccc
     3601 tcgtcatcga gcgacccgcg ccggtcgagc cgagcgtggt gctcgactac accgagctgc
     3661 accgcgacgc cgacctgctg gtgctcgaca agcccgccgg cctgccggtg cacccctccg
     3721 cccgctatca ccaccacacg ctgacggcgg tgatgcgcgg gcgcatgggt cggggccacg
     3781 gctgggacat ggcgcaccgc ctcgaccgcg agacctccgg cgtgctgatc ttcgggcgtc
     3841 acggcggctc ggcgagcgtg ctcaagcggg cgttctcgga ccgcacggtc gagaagcgct
     3901 actgggcgct gtgcctcggc cgcgtctccg aacctgtccg tatcgacatg cccctcgggc
     3961 cagccaaggg ctcggagatc catatcaaga ttggcccgcg cgcgctcgac gacggcggcg
     4021 tgcatgcggc gaccggggtg acaccgctgg cccacggcga gttccgcggc gcgccgatca
     4081 ccttgatcga ggtcaggccc gagaccggtc ggcagcacca gatccgcgtg cacatggcgg
     4141 agatcggcca tccgctgctg ggagacaagc tgtacgggct cgacgaggct cgcttcctcg
     4201 cggtcgtcga cggcgagcgc tccgggtcga cgacgccgcc tgcgctggcg ctggcgaagc
     4261 tcgaggcgga gctcgggctg gggcgccacg cgctgcacgc ggtgtcgatc tgcttcgatc
     4321 acccgaagac cggcgagcgc gtcgagttca cggcgccgtg gcccgccgac ctcgcggaga
     4381 tcatgccggc gccgggctga tcggctgatc agcgggcgag cagagggttg gcgccgggcg
     4441 cgagctcctc gaccacttcg ccaaagatca ccttgagcgc gacggtcttg ccgtcctcga
     4501 cctcgacctt catctgcgcc tgatcactgc cgtcggcgag tcgcaaggtg cggtccggca
     4561 gcgagatctc gaggacgccg ggcggcttgc ggtcgagccc gaggcgcgcc ttgccgccgg
     4621 tccaggtcat cacccgcggc cgaccctcga ggtcgctgcc gggcggaagc tcgaactgac
     4681 tcgagtgctg cggcggccgg ttgcggacgt accagaccat cagccccacg ccgcccgcca
     4741 cgagggcgac cgcgaggatg ccgaagatga tgcgcatgcg acggcccgac tgatgaccac
     4801 cgagccggcc gcggtggtag tcgccgaccg gcgggttgtc catcagctcg tcgtcgcgtt
     4861 cacgcggcgg cggcaaggtc gtgcgccgct cctcgccggc gaggtcaccc tcgaggcgca
     4921 cgcgtggctg ctcggacaac gggtcgcgca gcgggtcggc gagcgggtcg gcgaggtgct
     4981 cgcgcgcgag ctgtggcgtg ggagccagcc cggcgctgtc gaggtcgctt tgttcgcggt
     5041 cggcgggcat cggctcgagg gtagatcgag gtcggctgcc acggggcgtc ggcggcgcaa
     5101 aatgccgtgg ggagggcgta taacgccgtc caggcgcggt gcggcgggcg cggctcgtgt
     5161 gagtgcgtgt cagccgcgcc cggtcgcgag cgaccgcata tccgcgtgtc gggtcgccgc
     5221 ttcgaggcct cgcggccatc cggtcacggc gtgccagagc acgcccgcgt catcgcatga
     5281 acgggccacg ctgcctggcg cggcgtggca cgaccgcttc agatgaacgt gcggtcgtgc
     5341 gggagccagg ccgggcggac ctgggcccac atcacctggg cgacgaggcc gagcttgttg
     5401 ctggcgaggt gcatcgcgcc gctgacctgg cgcaggcggt cgcgcgtcgg gaggtgggcg
     5461 gcgtggcggg cgaccaggct cgactcgatc gcgtagcggg cggccttgag gtactcggtg
     5521 aggaacacct gcgggcggcc ctcggcgcac gagagcaggg ccatcgagcg ggcgcgggcg
     5581 gtcttgccgg aggcccagta ctcgaacagc gcccgctgga tcgctcgggc gccctcgttg
     5641 ccgccgcgga acacctcgta gatggcgtcg gcgagggcct ggcgggtcgg gacgtagcgg
     5701 tcgtgctcgg cgcggtagcg acgcaggctc gagtcgtcga cccacgcctc gtgcaccggc
     5761 gcgtcctcgc ggcggcgggc ctcctcgccg agacactcgg cgtcgcgcag gcccatcgtc
     5821 atgccgctgg cggtgatcgg gtgcgagcaa cccccggcgt cgccgatcag ggcgagcccc
     5881 ggctggaccg cggtcggtgc gggcaggttg acggtcgggg ccatctcgaa ggggcccttg
     5941 tggctggtga tcgcgtgggc gatctggtcg gccagaggtt gggggaggaa cggcacgaag
     6001 gtctcgagca ggaactcggc caggcgcgcg cccttggccg gcaagttggc cgggaggtcg
     6061 agggtgacgc ggtacagcag gtcgccgttg ggctcgtggg agatcgggta cacgagcacc
     6121 gggccccacg cgccgaggat gatgttgccc cacgtgcgcc cgggcagctt ggcgttgcgc
     6181 agctcgagac cgacggtgaa gccgacggtc tggcgctggt cggggattcc ggcgagcttg
     6241 cggacctggc tggccttgcc gtcggcgccg atgacgaggt cggcgtggat cttgacggtg
     6301 cgattaccgg ggcccgtcag cacgacgcca gcgacgcgct caccggagcg cacgaggtcg
     6361 gtgatgatcc agccctcgaa gaggtcgacc tgcgggcgct cgcggacgac ctggcgcatc
     6421 gtcttgacga gcgtcttatg gtgcgaggcg aggccctggg tgcggtactc ggggatgtcg
     6481 tcgtaaggga gctcgacgta gcggccgtcg gcgcgctcga gcacggtgaa gccgtcgaca
     6541 tcgacggcgc ccgcggcctt gagcgcgcca taaaaaccga ggtcgtcgag gatctgcgcg
     6601 ccgcgggggt ggatgaactc gccagcgaag cgcggcgagg atccggggtg gcggtcgatc
     6661 agcaagatcc ttcggcgacc ggaggtgtcg gcgagcgcga gcgcctgggc ggccgcacag
     6721 ccggcgatgc ccgccccgat gatcacgaca tcgtatcggc tggccgggga caggtcggac
     6781 agttcggtcg agtgcttgat cgctgcgttc atggggttcc tcggacgtcg ggatgagaca
     6841 gtcaatgcga agtgagtcag atctggccgc cgtgctcggc gaagagagtc agggccagcg
     6901 cggcctcgcg gacgcctgcg ctcgggtcct ggcacatcgg ggtcaacccg agaccggcgg
     6961 tccaggcccc gccgagccgc tccgacagct ctgcttgtgc cagctcgggc ggggcgttgt
     7021 ccaacaactg gaccaggtgc cgggcggcgc gctgggtctg gatgcaagcg gcgccctcga
     7081 gcgccatcag gccctgcatc gccaccgcgg tgtcgacggc gctgccgaag ccgccatcct
     7141 cgcgctggcg cccgcgaagc tggcgctcga tcgtctggcg caaggggtgg gtcggcgggc
     7201 acagggccgc ggtgcacagc gcgagctcgc cgagcgtggc gaggtcgaag cccgccatgt
     7261 cgagggtgac gcgctgccac aaccattgca ggccgcgcgc gacgcggtcg tcgtcggcct
     7321 ggaagccgag ccgcgcgagc tggcgtaacg ccgtcgccga gacgcgcacg cgggcgaggt
     7381 cggtcggcgt gccggcggcg agcaaccggg cgtcgcgcag cgggaagcgg gtcatgaaga
     7441 cctcggactc gccgcgctcg aagcgagcga agccgccgtc gggctcctgc atcgcgaaca
     7501 gcacctcgac ggcgcggcgc atcgcggcgc ggacctggct ttggtgtggg gaggtcttgg
     7561 gcagcgcgtg gagggcgtcg aggacggcgc aggtcgagag ggtgtcgacg tacttgtccg
     7621 cgccggcctc gcagggccag ccgccggcgg gcgcgttggt gcgaagctgc cactcgccga
     7681 cgtgccggat ctcctcggcg aggaagaacg gcaccgcggc ctcgccggcc gggtcgccgc
     7741 tggcgagggc gaggcggacc aggcgggcga ccgtcgtcag cggggcgcaa gtgaggccct
     7801 gggccagctg cgggcccgcg ggcgtgtgca cgaggccgcg agcgcgacgc atgtacgcga
     7861 ggccgcggac gatccgcggg tcgtcgtagt tggcgccgag cacccgtagg gcgatcagcg
     7921 agagcagggt cgggtgcgcc gcgaaccacc cgccatgggc ctgctgggcg gcgatcatcc
     7981 agcgttgcac gcggccgagc gcggcggtca gcaccgggcc gcgcgggagc ttgcggatca
     8041 gcttcacgcc ctgatcgaag gcctgcagca gatcccccag caggcccggt tggaccagcc
     8101 tcggcaagat cggctcgccg tcggggcggg tcagcagcag cgtcgcggcg ttgctgacgt
     8161 ggacccgcgc tggcgcgcgc gagagcagca ggtacggggt catcacgccg cgcgcccacg
     8221 gcgagacgcg gctcggcgac agccaggtga cggtcggcag cagccacagc tcggccggca
     8281 tcgtcggcag ccaggaccac gggatgtggc cggccatcgc catccacagg cggacctcga
     8341 tgctggcgcg ctgggccccg ccgagctcgt ggacgatgcg acgcgcgcgg acgaggtgct
     8401 cggcggccgg gtcgtcgccg gactcgacga gcgcccacca gccgagcgcg gtgcgtgaca
     8461 ggtcgggtcg accggcgggg tcgtgccagg cgccggaggg ttgctggctg gcgcggaccc
     8521 agccggcgag gctctcgcgc tcggcggtgc cgaggtcgtg gaacagtcgg tcggcgagca
     8581 ggacctcgca ggcgaggccg atgtcgggga tgctcagcag gtagggcgcc gcgccatcgg
     8641 tgtgcggctg tctatcgggc tgggcggggg tttgttggtg ccaccaggca gcggcagcgg
     8701 cggcccgggc gatcaccgcg cggaggcggc cttgcgtggg cacacggatc ggagagagag
     8761 caggcatacg gagaatcagg cgatctgcgc gaccaggacc cggcgcaggg cttacgaagc
     8821 taacatgcgt tcaggtacgc cccgagtgac acggatcaca ttcgggaccg ggcccgactc
     8881 ccgaaagacg gccatagaac aacgttttcg agggtgaaag ccgcttcgcg gtggcaccgg
     8941 gtctgcacgc tgttcggcga acttcgcgca gcctgcgaat acacggaact tgcggtggcg
     9001 tgtccgggtt tgcaagactg gcgacgatga acgcacaagc gcgcgcgctg ctgatggctg
     9061 gctgtatggt cggcggactg tcgttgatgg gctgcaagga ggaggagtcc gagagattcg
     9121 gcttcacctg catcgacctc aaccagggtg agggcatcaa ggaggatccg ttcatcggga
     9181 cgtaccggat catcgtcacg ctgaactacg agccgtgcat caaggacttc tacctcaaca
     9241 agcaccccga gatgcgtcag gacggcaagg aggggccggc ggtgttcgag gagtggaagg
     9301 agcgcctgtg ctcggaggac gtcgacgggc gcatcgagtg cgaggtcgag gagttcaagc
     9361 agacgctgat gtcgaccggc accgaggcat acaagatgtc ggtctcgtac atcacgccga
     9421 agtcggacca gatcgccggc cgcacgctgc tgtggggtcc cgggccgctg ccggagttcg
     9481 ccgagtgtga cgagggcctg cagcccttcg tcaacctgtc ggcgctgtcg ggcgtcatcg
     9541 gcgaggacaa ggacggcaag acgttgtggc aggtgcagtc ctttggtgac atgcgcggcc
     9601 tcatgcagac tcgtggcggc ggatgcatcc aggcgaacat ccagcccgtc ggcggcgtct
     9661 gatctgctcg gtcgcggcgg gcacgctgct cgccgcgtgt aacccacccg cggtgcgcga
     9721 gacggcgagc gtgacggagc cgccgagcgt ggcgctggcg ccgacctgcg cgccgccgcc
     9781 ggccgcggcg ctggccctga tcgccgacgc gccgtgtccg tggatcctcg tgtcgggaga
     9841 tcgctcggag atggcgctgc gggcgaccga catggtggca ccgcgggcgc tggtggtcgt
     9901 gccgccggag gcgtgcggcg atcgttgcag cttcaccggg gcgagcaccg ccctggggcc
     9961 cgtggtgctg gcgacccggc acgacccggc gagcgagctc gcggacgcgg cgttcatcgg
    10021 cgcggcgctc ggcggcggca cgctgcgctt cacgccgctg tggtacgggg cgagcgcgca
    10081 cggcgacagc accgacctcg gtccgagcta cgcgctggtg ccgtgggtgt gcgaggaggc
    10141 gctggtgctg gcgatcggcg ggcgcctgcc cggcgcgcgc gcggaggagc cggcgcgcgc
    10201 gctcgcggcc gccgcggggg tctacgcgat cgccgacgac gagctgcgac gaaccgacca
    10261 ggcgatgccg gcgctgaccg gatgcacgcg cgtaccgctg gagctgccat gaccgagatc
    10321 tctgtccccg aggacatgtc gcaagagcca gcacgtgacc tcggcctgac gctcgccggc
    10381 ggcggcagtc ggtcgtttta tcaggtcggc ctgctcgacc gctggggcga gcagctgcgg
    10441 ccgcggctgg cggcggtcgc gggggtgagc gccggggcgt gcacgggggc ggtgtggctg
    10501 gcggggcgca cggaggcgac gcgcgagtac tggcactggc ggcggcgcga cgtgcattcg
    10561 aacttcgagt ggcgccggct gctacgcggc gagcggccga cgccgcaggg tccggtgtac
    10621 cgcgacgtgc tggtgcacgc gtttcaagag ggcgggctcg agcagctgcg cgcgagcgag
    10681 atcccgatgc tggtgatggt cgcggtgccg ccgcggctgc tgcacccgct cgcgggcatg
    10741 ctgttcggct acggggtgta catggccgag aagaagatct ggccggcgac gctgcacccg
    10801 cgcgcggggc gaaagatcgg gttcgtgccg cgggtgttcg acgcgcgcct gtgcgagagc
    10861 ccggaggagg tcgccgagct gatcctcgcg tcgtcggcga cgccgccgtt tacgccgttc
    10921 gggcgctacc gcggcgagcg ttacctcgac ggcggcatgg tcgacaacgt gcccgccttc
    10981 gcggtcgagc agatcgcagg ggtgcgccgc aacctggtga tgatgacgcg accgtacccg
    11041 gcgcgcagcc tggggatcca tgggccgcgg ctgtacgtcg cgccgagcgg gctgacgccg
    11101 gtgacctgct ggg
//
LOCUS       DASWNJ010001087         2708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_478432, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001087 DASWNJ010000000
VERSION     DASWNJ010001087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478432"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1680
                     /locus_tag="VGB85_18330"
     CDS             <1..1680
                     /locus_tag="VGB85_18330"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0136053.1"
                     /translation="QRLYGAACEARRRGLRTSRALDLQATCAERRRGALAALVGVLRE
                     ADATAVERASQAASRLPAVDRCSDFAALTSEAEPGATRDPIVLARIESQHERLALARA
                     ELEAGHYARARELTQAVVEESEQLADDPLIAEALIARAAAELALGDYRATGATLHRSF
                     TTAEVAGQDDLAADAAVRLVHLHGEREHDLARAETWAELAEAKLRRAGDDGVQRPRLL
                     LYLGQTLAAAGRLQDAQPLLDQAMTLTERRRDRDPTSYVMALNASATLAMDKGALDVA
                     EPLLRRSLALREANLGPEHPSVAVARHNLGRLLYERRDFSRAAAEFRRALEIREHSLG
                     PEHPGTVQTLNGLGAALSDNGDREAGIEIFRRVLAIEERRRGVDHLDLFAPLNNLGQV
                     AINAGELATAADYLERARALLERQGQGQSPDQALVLYNLGALRLAAGDPAEAVTLLER
                     SLILRDQIYGPEHPEVGTSLALLSEAEFAAAIHDRATIHAERAITVLRKRPAGALELG
                     RARLVLARLRWRDPRTRDEARTLAREALKDLTSEARDSEQLREVRLWLATH"
     gene            complement(1691..2488)
                     /locus_tag="VGB85_18335"
     CDS             complement(1691..2488)
                     /locus_tag="VGB85_18335"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0136054.1"
                     /translation="MVERLDATVAEHGGTCSPASEDSGHLDTGTSSSTSTGEVDGSGT
                     EGHSGSAGTSTTSAAETQAGSGGSSSSGGGPVCGDGVVEGDETCDDGNATPDDGCQEC
                     ARDSIVFITSESYQGYALEGLYGADQRCRSLAAKAYLGRPESFRAWLSTPSMAAADRL
                     LHSRGRYVLTNGLVVAQNWDELTSGALQNPIVVDESSQTKEDYVWTGTLPDGQPALGS
                     EFCGEWKETMGFLIFGGMGRALETDGKWSFVEQGGCGLDSSLYCIEQ"
BASE COUNT          386 a         1142 c          822 g          358 t
ORIGIN      
        1 caacgcctct acggcgccgc ctgcgaggcc cgccgtcgcg gcctgcggac cagccgcgcg
       61 ctcgacctcc aggccacctg cgccgagcgc cgccgcggcg ccctcgcggc gctcgtcggc
      121 gtgctgcgcg aggccgacgc gaccgcggtc gagcgcgcca gtcaggccgc gagccggctg
      181 ccggcggtcg accgctgcag cgacttcgcg gcgctgacca gcgaggccga gccgggcgcc
      241 acgcgcgacc cgatcgtgct cgcgcgcatc gagtcgcagc acgagcgcct cgccctcgcg
      301 cgcgccgagc tcgaggccgg tcactacgcg cgcgcccgcg agctgaccca ggccgtcgtc
      361 gaggagtccg agcagctcgc cgacgaccca ctgatcgccg aggccctgat cgcccgcgcc
      421 gccgccgagc tcgccctcgg cgactaccgg gccaccggcg ccaccttgca tcgcagcttc
      481 acgaccgccg aggtcgccgg gcaggacgac ctcgccgccg acgccgcggt ccgcctcgtg
      541 cacctgcacg gcgagcgcga gcacgacctc gcgcgcgccg agacctgggc cgagctcgcc
      601 gaggccaagc tgcgacgcgc cggcgacgac ggcgtccagc gcccgcgcct gctgctctac
      661 ctcggccaga ccctcgccgc cgccggccgc ctgcaggacg cccagccgct gctcgaccag
      721 gcgatgaccc tcaccgagcg ccgccgcgac cgcgacccga cctcctacgt catggcgctc
      781 aacgccagcg ccacgctggc gatggacaag ggcgccctcg acgtcgccga gccgttgctg
      841 cggcgatcgc tcgcgctacg cgaggccaac ctcggccccg agcacccgag cgtcgccgtc
      901 gcgcgccaca acctcgggcg tctgttgtac gagcgccgcg acttctcccg cgccgccgcc
      961 gagttccgcc gcgccctcga gatccgcgag cacagcctcg gccccgagca ccccggcacc
     1021 gtgcagaccc tcaacggcct cggcgccgcg ctctccgaca acggcgaccg tgaggccggc
     1081 atcgagatct ttcgccgcgt gctcgcgatc gaggagcgcc gccgcggcgt cgaccacctc
     1141 gacctgttcg ccccgctcaa caacctcggc caggtcgcga tcaacgccgg cgagctcgcc
     1201 accgcggccg actacctcga gcgcgcgcgc gcgctgctcg agcgccaggg ccagggccag
     1261 agccccgacc aggcgctggt gctctacaac ctcggcgccc tgcgcctcgc cgccggcgac
     1321 cctgccgagg ccgtcaccct gctcgagcga tcgctgatcc tccgcgacca gatctacggc
     1381 cccgaacacc ccgaggtcgg caccagcctc gcgttgctca gcgaggccga attcgccgcc
     1441 gcgatccacg accgcgccac gatccacgcc gagcgtgcga tcaccgtcct gcgcaagcgc
     1501 cccgccggcg ccctcgagct cggccgcgcc cgcctcgtgc tcgcccgcct gcgctggcga
     1561 gatccaagga cccgcgacga ggcgcgcacc ctcgcccgcg aggccttgaa agacctcacc
     1621 agcgaggcac gcgactcgga gcagctccgc gaggtccgcc tgtggctcgc gacgcattga
     1681 tcggcgaccg ctattgctcg atgcagtaga ggctgctgtc gagaccacaa cctccctgct
     1741 cgacgaacga ccacttgccg tcggtctcca gggcgcgccc catcccgccg aagatcagaa
     1801 agcccatcgt ctccttccac tcgccgcaga actccgaccc cagcgccggc tgtccatccg
     1861 gcagcgtccc ggtccatacg tagtcctcct tcgtctgcga gctctcgtcg acgacgatcg
     1921 gattttgcag cgcccccgag gtcagctcgt cccagttttg cgcgaccacg agcccgttgg
     1981 tcaggacgta ccgaccccgc gagtgcaaca aacgatcggc cgccgccatc gatggggtgc
     2041 tcagccacgc ccgaaagctc tcgggccgcc ccagatatgc cttcgccgcc agactgcggc
     2101 accgctgatc cgcgccgtac agcccctcca gcgcgtatcc ctggtagctc tcgctcgtga
     2161 tgaacacgat cgagtcccgc gcgcactcct gacacccatc gtccggcgtc gcgttcccgt
     2221 catcacaggt ctcgtccccc tcgaccaccc catcgccgca gaccggcccc cctccgctac
     2281 tgctgctccc ccctgagccg gcctgcgtct cggccgcgga ggtcgtggac gtcccggccg
     2341 acccgctatg gccctccgtc ccggagccgt ccacctcgcc tgtgctcgtg ctcgacgatg
     2401 tgcccgtgtc gaggtgtccg ctgtcctcgc tcgctggcga gcaggtgccc ccatgctccg
     2461 cgaccgtcgc gtcgagccgc tcgaccacac agcctgggcc aacgagcgcc gcgacgaccg
     2521 cgacccggac ggcgcggcga attcgatgtt ctgaatgacc gcgcataatt cgaccgtcct
     2581 ctcattgctc gatgcagtag aggctgctgt cgagaccaca atctccctgg tcgacgaacg
     2641 accacttgcc gtcggtctcc agggcgcgcc ccaccccgcc gaagatcaga aaacccatcg
     2701 tctccttc
//
LOCUS       DASWNJ010001088         1525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_656048, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001088 DASWNJ010000000
VERSION     DASWNJ010001088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656048"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /locus_tag="VGB85_18340"
     CDS             <1..1393
                     /locus_tag="VGB85_18340"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEY0136055.1"
                     /translation="LGAAASPTALARDCLPRRFAGAVHGMHVEREPLGAMSRAIDLPH
                     AAPRDPGRPCGITPAEIRARYAIPADQHAAGETIALMALGGLPDRRDLEDSWAAWGVP
                     APELHARQVGGGTISQHPLHILETTMSLAWLGAMAPGARLVVYFIDPERCPDPWVTFL
                     QAVIADDDLRPTVATTSWSTPEYQYYRTHGRETFASLLDQAAALGITVLAASGDWGAL
                     AGFPRMLADGRRACVAAWPHAVFPAVEARVLAVGGTMPCADDPAQEEAWNVRVSPEIR
                     RHLGLGRLASSGGFSELVPIPAWQAPLLRPTYARTDGPAVIPDGRGYPDVVLLAGGGA
                     DIPDSGFGLVLGGRWCDDGGGTSLTAPIWATIVAGLNHARREQGLPRLGDCTGKLHDL
                     AARGTGFTPIARGNIDLVVPALDSGELTPHRVAGYVPTPHWNPATGLGTPRVDELRRA
                     LTEPVHEASQAHA"
BASE COUNT          205 a          629 c          489 g          202 t
ORIGIN      
        1 gctcggcgcc gccgcgagcc cgaccgcgct ggcccgcgac tgcctcccgc gccgcttcgc
       61 cggcgccgtg catggcatgc acgtcgagcg cgagccgctc ggcgccatgt cgcgtgcgat
      121 cgacctcccc cacgccgcgc cgcgcgaccc gggccgcccc tgcgggatca cgccggccga
      181 gatccgcgcc cgctatgcga tccccgccga ccagcacgcc gcgggcgaga cgatcgccct
      241 gatggcgctc ggcggcctgc cggatcgccg cgacctcgag gacagctggg ccgcgtgggg
      301 cgtgccggcc cccgagctgc acgcccgcca ggtcggcggt ggcacgatct cgcagcaccc
      361 gttgcatatc ctcgagacga cgatgagcct cgcgtggctc ggcgcgatgg cccccggcgc
      421 gcgcctggtc gtctatttca tcgaccccga gcgctgcccc gacccctggg tcaccttttt
      481 acaggcggtg atcgccgacg acgacctgcg cccgaccgtc gccacgacct cgtggagcac
      541 ccccgaatat cagtactatc gcacgcacgg ccgcgagaca ttcgcgtctc tgctcgacca
      601 ggccgccgcg ctcggtatca ccgtactcgc cgccagcggc gactggggcg cgctcgccgg
      661 cttcccgcgc atgctcgccg acgggcgccg ggcctgcgtc gccgcctggc cgcacgccgt
      721 gttccccgcc gtcgaggcgc gcgtgctcgc ggtcggtggc acgatgccct gcgcggacga
      781 ccccgcgcag gaggaggcgt ggaacgtgcg agtctcgccg gagatccgcc gccacctcgg
      841 cctcgggcgc ctcgccagca gcggcggctt cagcgagctc gtgccgatcc ccgcctggca
      901 agcgcccttg ttacgcccca catatgcacg caccgacggc cctgccgtca tccccgacgg
      961 ccgcggatac cccgacgtgg ttctgctcgc cggtggcggc gcggacatcc ccgacagcgg
     1021 cttcggcctg gtcctcggcg gccgctggtg cgacgacggc ggcggcacca gcctgaccgc
     1081 cccgatctgg gccacgatcg tcgcgggcct caaccacgcg cgccgcgagc agggattacc
     1141 gcggctcggc gactgcaccg gcaagctcca cgacctcgcc gcacgaggca ccggcttcac
     1201 accgatcgcc cgcggcaaca tcgacctcgt cgtgcccgct ttggactccg gcgagctcac
     1261 cccgcatcgc gtcgcgggct atgtcccgac accgcactgg aaccccgcga ccggcctcgg
     1321 caccccccgc gtcgacgagc tgcgccgcgc gctgaccgag ccggtccatg aagcctcgca
     1381 agcacacgcc tgacgcctgt acgcccgccc ggcaccgcat cagctccgcc tgcgtgcgcc
     1441 tcacggcctg cacgcgaata atgacggttg aacgacgagt atctgaaact cgcacgtgca
     1501 ccacatcacc aatgtgatac ggcta
//
LOCUS       DASWNJ010001089         1674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_402257, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001089 DASWNJ010000000
VERSION     DASWNJ010001089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402257"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VGB85_18345"
     CDS             <1..474
                     /locus_tag="VGB85_18345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136056.1"
                     /translation="VLGVNDGMSDLGISPVGSSKNGTNTGTSFGMLGWIPWVLGVLLL
                     ILTIAVVRLMLTRRAIAADRARSEAMLVSILRAVKSGDDDEVPGPADFNTVIARARQQ
                     FPNVDDHDSYLAHIITRASLPTRPTAQVRAKLPTDNPYADAAVAAAKKQPPPPAA"
     gene            complement(501..893)
                     /locus_tag="VGB85_18350"
     CDS             complement(501..893)
                     /locus_tag="VGB85_18350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136057.1"
                     /translation="MSRSIARLWPVVLGIATLAAMYMNWRGDPFDPTLEGTARYGHNH
                     EGALSQIASLVLAELVVLYLVLAPGSAARSIGRAIAALLLFAPWTLFSLLLTMHAGGI
                     AALHALWLMAVDLGIVVVLVARCVKRRQ"
     gene            1131..1538
                     /locus_tag="VGB85_18355"
     CDS             1131..1538
                     /locus_tag="VGB85_18355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136058.1"
                     /translation="MRGLVAILALASACDRVAYGSSADVADILAAHGVTAPSLVCRNP
                     AYEDGAIVRAVACTTTLTPAEVETLRATVPLAPGTPRSSAPVHNCATRIAARAEVLVG
                     ANTRVANGVGPIALYHDRTSGEACVEITYPWSE"
BASE COUNT          261 a          647 c          562 g          204 t
ORIGIN      
        1 gtgctcggcg tcaacgacgg catgagcgac ctcggcatct cgccggtcgg ctcgagcaaa
       61 aacggcacga acaccggcac cagcttcggc atgctcggct ggatcccgtg ggtgctcggc
      121 gtgctgctgc tgatcctgac gatcgccgtc gtgcgcctga tgctgacccg ccgcgcgatc
      181 gcggcggacc gggcccgcag cgaggcgatg ctggtcagca tcctgcgcgc cgtgaagtcc
      241 ggcgacgacg acgaggtccc cggcccggcc gacttcaaca ccgtcatcgc ccgcgcgcgc
      301 cagcagttcc cgaacgtcga cgaccacgac tcgtacctcg cgcacatcat cacgcgcgcc
      361 tcgctgccga cccgcccgac cgcgcaggtg cgcgccaagc tgccgaccga caacccctac
      421 gcggacgccg cggtcgcggc cgccaagaag cagccgccgc cccccgcggc gtgacccggg
      481 gtgcaggtct cgggcgcggg ctactggcga cgcttcacgc agcgcgcgac gagcacgacg
      541 acgatcccga ggtcgaccgc catcagccac aacgcgtgca gggcggcgat cccaccggcg
      601 tgcatcgtca gcagcagcga aaacagggtc cacggcgcga acaacagcag cgccgcgatc
      661 gcccgcccga tgctgcgtgc cgccgatccg ggcgcgagca cgaggtacag caccaccagc
      721 tccgccagca cgagcgacgc tatctgcgac agcgcgccct catgattatg cccgtaccgc
      781 gcggtgccct cgagcgtcgg atcgaagggg tcgccgcgcc agttcatgta catcgccgcg
      841 agcgtcgcga tgcccagcac caccggccac aggcgcgcga tcgaccgact catcatggca
      901 gcatatcacg ccggtccgac cggactcggc ccgcgaagcc gggccgacct gccgcttcga
      961 cacgaacgcg agcgtcgccc gggtcgtgcc gccgtggaag ttctcgccag cgccgaacag
     1021 gctcgccgac gcgccggtgg acgtaaaacc gggctggcag ggagacggac cgacgtgatg
     1081 gcggccggct ccgcctcccc cggcggcccg ggtcgcgtat cctttgcagc gtgcgtggtc
     1141 tcgtcgccat cctcgcgctc gccagtgcgt gcgatcgagt cgcatacggg tcgagcgccg
     1201 acgtcgccga catcctcgcg gcccacggcg tcacggcgcc ctcgctcgtg tgcaggaacc
     1261 cggcgtacga ggacggcgcg atcgtgcggg ccgtcgcctg caccaccacg ctgacgccgg
     1321 ccgaggtgga gacgctgcgc gcgaccgtgc cgctcgcacc gggcacgccg cgcagctcgg
     1381 cgcccgtgca caactgcgcg acgcggatcg cagcgcgggc cgaggtgctc gtcggcgcca
     1441 acacgcgggt cgcgaacggc gtcggtccga tcgccctgta ccacgatcgg acgagcggcg
     1501 aggcctgcgt cgagatcacc tatccctggt cggagtgagc cgcatgaaca cgatgaaccc
     1561 cgaagatcca ccccgcttca gcttcctcgc gccggactgc ccgcgcaccc tcggcgcgtg
     1621 catggccgag ttcgcggcgg cgcagccggg gctgctcgcg cccgcggacc ccga
//
LOCUS       DASWNJ010001090         3400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_478485, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001090 DASWNJ010000000
VERSION     DASWNJ010001090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478485"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..3229
                     /locus_tag="VGB85_18360"
     CDS             143..3229
                     /locus_tag="VGB85_18360"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY0136059.1"
                     /translation="MQAPRELSPGADVAGAAEARLWAALAAMVCGEAELAEAWLVAAV
                     DLLAGSSGQAGLQARVAQLRGNLEHLRGRVDAAVVWYERAAADFASAGEAIGRTLLAA
                     NLAALAIVGGDVGRGLEHGRAALRGYLSLGRVQALPELAVNLVQLLVKAGSLAEARGL
                     ALLLHRLLAGGVGGPLAQARRARVDAELAFGAWLGGERVDVFAAFASAAQQLAEAGAG
                     REAVEAGLRASALARRAGRLDLAARHLAAAQAIGGELHDDDLVLSLALESLALASATG
                     GRESFAAACAALASQPRPEALAAQGRLELAWRYDQVLSAVMPPGDRRRRALAQRMQAT
                     WDALMRKIPPLERAAVRTALYAESGERATTPRSGGAPFVGDTRNETRTARATTSERVP
                     GEASRDERATAGATAPSEAIPSTAPREAATATSSAAPRGPVTAMSSAAPREPAPVAAR
                     HDDESMRGVGREERAMSDPSEPREVSDDDVRLPDPRAALADVLGDLSETTAEPSRERS
                     DERDNRHERLIRLYRRLAREDDLRRLLAQVVDAVMDLCDAERGVVVVAAGGEQMEVAR
                     ELAGSAGVAFSRSIIDRVLGQGAPVLSVDAVADDRFDGARSISHLNLRSVLAVPLLHR
                     GEAIGAVYVDHRLRRGAWGEADLVLVEEFAELAALAIAHARTLAEQRAQAEALVAQGS
                     ALAKLLEERELEVRGLREAVRTPDRQVYRGMVGGTSAMQAVFRLIDRLADSDVPVVIY
                     GESGTGKELVARAIHEAGARKGRPFIAENCGAIPEALLESVLFGHAKGAFTGAHRATP
                     GLFEAADGGTIFLDEIGEMSPGMQTKLLRVLQEGEVRRVGDTAVRKIEVRVIAASNRD
                     LEAMVAGGQFRKDLLYRIRVVKIDLPPLRSRPDDLASLIEHFLGRHDRQRRLTVSGAA
                     MRALARYAWPGNIRELENEVQRWVALVESRVELADLSPAIVEARGDGGRDPDDLRLRP
                     RLDRLERELIERAMTTAHGNQTRAATLLGLSRFGLQKKLRRLAEGTPLVDDDEGAP"
BASE COUNT          447 a         1165 c         1368 g          420 t
ORIGIN      
        1 gaggggtgcc gcggaggtac cgcgtggcct gcccgcgggg acaggtacga aaggggccgc
       61 ggaggggggc ctgtccgcgg ggacaggtac gaagtgggcc gcggaggggg gcctgtccgc
      121 ggggacaggc acgaaaggga ccgtgcaggc gccgcgcgag ctgtcccccg gggcagatgt
      181 cgcgggggcc gcggaggcgc ggctgtgggc cgcgctggcg gcgatggtct gcggggaggc
      241 cgagctcgcg gaggcgtggc tggtcgcggc ggtcgacctg ctcgcggggt cctcgggcca
      301 ggcggggctg caggcgcggg tcgcccagct gcgcggcaac ctcgagcacc tgcgcggccg
      361 ggtcgacgcg gcggtcgtct ggtacgagcg ggcggcggcg gacttcgcga gcgcgggcga
      421 ggcgatcggt cgcacgctgc tggcggccaa cctcgccgcg ctggcgatcg tcggcggcga
      481 cgtcggccgc gggctcgagc atggacgcgc cgcgctgcgc ggctacctct cgctcggccg
      541 cgtgcaggcg ctgcccgagc tggccgtcaa cctcgtgcag ctgctggtca aggccggctc
      601 gctcgccgag gcgcgggggc tcgcgctgct gctgcaccgc ttgctcgccg gcggcgtcgg
      661 cggtccgctg gcccaggcgc gcagggccag ggtcgacgcc gagctcgcct tcggcgcgtg
      721 gctgggcggc gagcgagtcg acgtgttcgc ggccttcgcc agcgccgcgc agcaattggc
      781 cgaggcgggc gcgggacggg aggcggtcga ggccggcctg cgagcgagcg cgctggcccg
      841 tcgcgccggt cggctcgatc tcgcggcgcg tcacctcgcc gccgcgcagg cgatcggcgg
      901 cgagctgcac gacgacgacc tcgtgctgag cctcgcgctc gagtcgctcg cgctggcgtc
      961 ggcgaccggc ggacgcgaga gcttcgcggc cgcctgcgcg gctctggcga gccagccgcg
     1021 accggaggcc ctcgcggcgc agggtcgcct cgagctggcc tggcgctacg accaggtgct
     1081 ctccgcagtg atgcctccgg gcgaccgtcg ccgccgcgcc ctcgcgcagc gcatgcaggc
     1141 gacctgggat gcgctgatgc gaaagatccc gccgctcgag cgcgccgcgg tccgcacggc
     1201 gctgtacgcc gagtcgggcg agcgcgccac gactccgcgg tcaggcggcg cgccgtttgt
     1261 cggtgatacc cgcaacgaga caaggacagc gcgcgcgacg acgagcgagc gtgttccggg
     1321 cgaggcatcc cgcgacgagc gggcgacagc aggcgcgaca gcgccgagcg aggccattcc
     1381 cagcacagcg ccccgcgagg ccgcgacagc gacgagcagc gcagcgcccc gcgggccagt
     1441 gacagcgatg agcagcgcag cgccccgcga gcccgcgccg gtcgcagcgc ggcacgacga
     1501 cgagagcatg cgcggcgtgg gccgcgagga gcgagcgatg agcgatccat ccgagccacg
     1561 cgaggtcagt gacgatgatg tgcggctgcc cgatccgcgg gcggccctcg cggatgtcct
     1621 cggggacctg tccgaaacga cagccgagcc gagccgcgag cgcagcgacg agcgggacaa
     1681 ccgccacgag cgcctgatcc ggctgtatcg ccggctggcc cgcgaggacg acctcaggcg
     1741 cctgctcgcg caggtcgtcg acgcggtgat ggacctgtgc gacgcggagc gcggcgtggt
     1801 cgtcgtcgcc gcggggggcg agcagatgga ggtcgcgcgc gagctggccg gcagcgccgg
     1861 ggtggccttc tcgcgctcga tcatcgatcg agtgctcggg cagggggcgc cggtgctcag
     1921 cgtcgacgcc gtcgcggacg accgcttcga cggggcgcgg tcgatcagcc acctcaacct
     1981 gcgcagcgtg ctcgcggtgc cgctgctgca ccgcggcgag gcgatcgggg cggtgtacgt
     2041 cgatcatcgg ctgcggcgcg gcgcctgggg cgaggcggat ctggtgctcg tcgaggagtt
     2101 cgcggagctg gccgcgctgg cgatcgccca cgcgcgcacg ctcgcggagc agcgggccca
     2161 ggccgaggcc ctcgtcgcgc agggcagcgc gctggcgaag ctgctcgagg agcgcgagct
     2221 cgaggtccgc gggctgcgtg aggccgtgcg cacgccggac cggcaggtct accgcggcat
     2281 ggtcggcggc acgtcggcga tgcaggcggt gtttcgcctg atcgatcgac tggccgacag
     2341 cgacgtgccg gtcgtgatct acggcgagag cggcaccggc aaggagctgg tcgcgcgcgc
     2401 gatccacgag gccggcgcgc gcaagggtcg gcccttcatc gccgagaact gcggtgcgat
     2461 ccccgaggcg ctgctcgaga gcgtgctgtt cgggcacgcg aagggggcgt tcacgggggc
     2521 gcaccgggcc acgccggggc tgttcgaggc cgccgacggc ggcacgatct tcctcgacga
     2581 gatcggcgag atgagcccgg ggatgcagac caagctgctg cgcgtgttgc aggagggaga
     2641 ggtgcgccgc gtcggggaca ccgcggtgcg caagatcgag gtgcgggtga tcgccgcgag
     2701 caaccgcgac ctcgaggcga tggtcgccgg cgggcaattc aggaaggacc tgctctatcg
     2761 catccgcgtg gtgaagatcg acctgccgcc gctgcgcagt cgacccgacg acctcgcatc
     2821 gctgatcgag cacttcctcg ggcgtcacga ccgccagcgg cgtctgacgg tcagcggcgc
     2881 cgcgatgcgg gcgctggcgc gctacgcctg gccgggcaac atccgcgagc tcgagaacga
     2941 ggtccagcgc tgggtcgcgc tggtcgagtc gcgggtcgag ctcgccgacc tcagcccggc
     3001 gatcgtcgag gcgcggggcg atggtggtcg agatcccgac gacctgcgac ttcgtccgag
     3061 gttggaccgt ctcgagcgcg agctgatcga gcgcgccatg acgaccgccc acgggaatca
     3121 gacccgcgcc gcgacgctgc tcggtctgtc gcgcttcggc ttgcaaaaga agctgcggcg
     3181 gctcgcggag ggcacgccgc tcgtcgacga cgacgagggc gcgccgtgaa tccgcgcgcg
     3241 gacccggccg cgtcccgcca accgggttgg cgacttacgg cgatctcgcc accgcggttg
     3301 tcgatcgcgg cgagcgacgg cccgcattcc tggagaattt ccgggcccga atattgcttc
     3361 gttatgggac gcggggacta aaacctctgc ctcgcgcctg
//
LOCUS       DASWNJ010001091         1707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_159392, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001091 DASWNJ010000000
VERSION     DASWNJ010001091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159392"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1680)
                     /locus_tag="VGB85_18365"
     CDS             complement(<1..1680)
                     /locus_tag="VGB85_18365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136060.1"
                     /translation="MPQAALDDRFVSGRELAKQQTRWQRNEQGESAASPMPMPDVTRT
                     TGSHFAPDFMTTESRGHASRIGGRGQPLALHYLGDARLGDLGEHVPALFEDNFDRERE
                     QLLGATAAAGGGKISEDARALLTRARASLGEARYAVDGGVLAIEAGGHFTRSRRIGGY
                     LREVVRYDGDTLVATYPELDLAVERRVGLAEPALLSQWVPWVLPSPDALARWYIVELL
                     DASTLRMRATEGDDIIDLRLDPQLRLAAITRRGGATVVGETRFEHDDHGLTIVVGDER
                     RRVEHTTDTPDLGDLAATTRLQLPLRSQSELDAALAQATPGSPAWRDLQRQLLAVLAA
                     LGQSYHQPAVVAAMRAHGPLTRGELVLAGAGVVNLDGRPGDQVLADLTGDPVAAYLGA
                     LRRLRKNATGPMEQVAKASPGTLVGLLASYTRLLADAPKGVTPAALGRLRDFFARYDH
                     PELAYIATHQLAAPIDWRRPGATVPAWQALATAHPTWAPVALHSAATAQQYAGKHVQA
                     SELYAAALAAAVDGAQIPVIDWNVRNALQHSSGGHAAWRMQWNRWRAAVQKS"
BASE COUNT          209 a          560 c          705 g          233 t
ORIGIN      
        1 ggacttttgc acggcggcgc gccagcggtt ccactgcatg cgccaggccg catggccgcc
       61 gctgctgtgc tggagcgcgt tgcggacgtt ccagtcgatg acggggatct gggcgccgtc
      121 gacggcggcg gcgagggccg cggcgtacag ctcgctggcc tgcacgtgct tgccggcgta
      181 ctgctgcgcg gtcgcggcgg agtgcagcgc gacgggggcc caggtcggat gggcggtcgc
      241 cagcgcctgc caggccggga cggtcgcgcc agggcgccgc cagtcgatcg gcgccgcgag
      301 ctgatgagtg gcgatgtacg cgagctcggg gtgatcgtag cgcgcgaaga agtcgcgcag
      361 gcgcccgaga gcggccggcg tgacgccctt cggggcgtcg gcgagcagcc gcgtgtagct
      421 ggcgagcagg ccgacgaggg tgcccgggct ggcctttgcg acctgttcca tcggaccggt
      481 cgcgttcttg cggagccgac gcaaagcgcc gaggtaggcg gctacggggt cgcccgtgag
      541 gtcggcgagg acctggtcgc ccggcctgcc gtcgaggttg acgacgcccg cgcccgcgag
      601 cacgagctcg ccgcgggtca gcggcccatg ggcccgcatc gcggcgacga cggcgggctg
      661 gtggtaggac tggccgagcg cggcgagcac ggcgaggagc tggcgctgga ggtcgcgcca
      721 ggcggggctg ccgggggtcg cctgcgcgag cgcggcgtcg agctcggact ggctgcgcag
      781 cggcagctgc aggcgggtgg tcgcggcgag gtcgccgagg tcgggggtgt cggtggtgtg
      841 ctcgacgcgg cggcgctcgt cgccgacgac gatcgtcagg ccgtggtcgt cgtgctcgaa
      901 gcgagtctcg ccgacgacgg tggcgccacc acgacgggtg atcgcggcga ggcgcagctg
      961 tgggtcgagg cgcaggtcga tgatgtcgtc accctcggtg gcgcgcatgc gcagggtgct
     1021 cgcgtcgagc agctcgacga tgtaccagcg cgcgagcgcg tcgggcgagg gcagcaccca
     1081 cgggacccac tgcgacagca gggccggctc ggcgaggccg acgcggcgct cgacggcgag
     1141 gtcgagctcg ggataggtgg cgaccagcgt atcgccgtcg tagcggacga cctcgcgcag
     1201 atagccgccg atccgccggc tacgcgtgaa gtgaccgccg gcttcgatcg ccagcacgcc
     1261 gccgtcgacg gcgtagcggg cctcgccgag gctggcgcgc gcgcgggtca gcagggcgcg
     1321 ggcgtcctcg ctgatctttc cgccacccgc ggccgcggtg gcgccgagca attgttcacg
     1381 ctcgcggtcg aagttgtcct cgaacagcgc gggcacgtgc tcaccgaggt cgccgaggcg
     1441 ggcgtcgccg aggtaatgca gcgcgagcgg ctgaccgcgt ccaccgatcc gcgaggcgtg
     1501 gccgcggctc tcggtggtca tgaagtcagg cgcgaagtgc gagccggtgg tcctcgtgac
     1561 gtcgggcatc ggcatcggcg aggccgcgga ctcgccctgc tcgttgcgct gccatcgcgt
     1621 ctgctgtttg gcgagctcgc ggccgctgac gaagcggtcg tcgagcgcgg cctgcggcac
     1681 ggtcggtccg aagctggcgg cgcccat
//
LOCUS       DASWNJ010001092         4353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_656132, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001092 DASWNJ010000000
VERSION     DASWNJ010001092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656132"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1825
                     /locus_tag="VGB85_18370"
     CDS             <1..1825
                     /locus_tag="VGB85_18370"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEY0136061.1"
                     /translation="LGAPTELGLAFPSDTVVQGFSNNADALLVSGLLADQYREAAETV
                     AAEIVKDLPARLGCDPVVDGEAECAAGFIARFGKQAFRRPLTATEAARYQTLWAEATK
                     AEGFGAGIQWTIAALLQSPGFLYRSELGDHVGDGVYTLTPYELASELSYLIVGGPPDA
                     ELIAAADDGTLTDLKVLQAQAVRLLALAESDATLHRFVDEWLHLDRLRTVPRDATLYP
                     ELTPQIRDAMLGETHRFVSSIYREGGTLADLLTASYSLMTDALAQYYDLPPGAGEPDA
                     AGYKRTDTAGAAGLLAHGGLLTTHALPTSSSPIHRGKLVRERLLCQDMPPPPPALDTS
                     PPPVDPSLSTRERYTQHSSDPTCAGCHTRIDPIGFGFEHYDAIGRFRLKDGVHDVDAS
                     GEIVLTDQTNGTFDGIDELAALLAGSPEVEACYTLQWSRHALGSAEASELACVQDEVT
                     AAFTGAEGRLDSLVLALVGTRFFRERGGAVTDDPGSETGSDTGSDTGSDDTGTTTTSG
                     GDTGSDTGDQPGPMTTPGIEAVLVQDSVWAAGECDTVTVTNTTDAPITWQAGVALKGT
                     IQNFWNAKYVAVDGGFVFTGEAYNAVVAPKTSTSFGFCLNY"
     gene            2127..2906
                     /locus_tag="VGB85_18375"
     CDS             2127..2906
                     /locus_tag="VGB85_18375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136062.1"
                     /translation="MPIRVRHRLTGLHLVALALTLPAPALAAPCPTIAGGSEALTEHD
                     SEARLAFIRARMKHDAWRARQWSLGYGTGYALLTGASAIAAPLIKDRASVPDLYVGGF
                     SSIIGFGLIAVSPLKVIFDREELEAQIEKAGPGGDRCALLATAEAMLIADAKNERFGR
                     SWLIHSGNVLVGVGALLVLGLGYDRWGSGIANGIASIAVGEVMIFTQPVGLVRDLKRY
                     RLGDLSQPTRRRKRIAWGGAPMVTPPGTGAGVYGLSLMGRF"
     gene            3001..4098
                     /locus_tag="VGB85_18380"
     CDS             3001..4098
                     /locus_tag="VGB85_18380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136063.1"
                     /translation="MRPVRLAVCILAVALACGGGSGGGFASSMGPAITSVSAGTDAGS
                     TSTTASSTSGDISSTSGEASSTSAASAASTGTIYDLGGGGDIGPIAPPGCKDRIDFLF
                     VMKRGLQLPEHQQKLLAAFPAFIDTIQDKFAGFDVHIMVVDTESWWGQQSCDPEGCLD
                     TDNNGCQIGEHFVADYPCGVKATLGKCDKTLGAGTVFNAGLDSANVPCKLDGGHRYIT
                     QDQTDLKATFACMAQVGQGGGENVGQATAEAVSPALNQDGGCNAGFLRDDALLMITFV
                     APGGDDDSDGTPQDWADAVFAAKHGDKNAVVMLGIHGVAPDGGRGRLVEFLELFPFSL
                     QGYLTEPSFGPIFADAVEMVDAACQGFTPPG"
     gene            complement(4122..>4353)
                     /locus_tag="VGB85_18385"
     CDS             complement(4122..>4353)
                     /locus_tag="VGB85_18385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136064.1"
                     /translation="YNVVVSAVSMGPGLNYALRVTGHRTDDVDLALSYLFDTPGLEMG
                     QAVAVGPLGYIWFGGVRSIGGVLHAVAGRGHG"
BASE COUNT          669 a         1664 c         1380 g          640 t
ORIGIN      
        1 cctcggcgcc ccgaccgagc tcggcctcgc cttcccgtcc gacaccgtcg tgcagggctt
       61 tagcaacaac gccgacgcgc tgctcgtctc gggcctgctc gccgaccagt accgcgaggc
      121 cgccgagacc gtcgccgccg agatcgtcaa ggacctgccc gcgcgcctcg gctgcgaccc
      181 ggtcgtcgac ggcgaggccg agtgcgccgc cggcttcatc gcgcgcttcg gcaagcaggc
      241 gtttcgccgc ccgctgaccg cgaccgaggc cgcgcgctac cagaccctgt gggccgaggc
      301 caccaaggcc gagggcttcg gcgccggcat ccagtggacg atcgccgcgc tgctgcagtc
      361 gcccgggttt ttgtaccgca gcgagctcgg cgaccacgtc ggcgacggcg tctatacatt
      421 gaccccgtac gagctcgcca gcgagctgtc gtacctgatc gtcggcggcc cgccggacgc
      481 cgaattgatc gccgccgccg acgacggcac cctcaccgac ctcaaggtgt tgcaggccca
      541 ggccgtgcgc ctgctcgcgc tcgccgagag cgacgcgacg ctgcaccgct tcgtcgacga
      601 gtggttgcac ctcgaccgcc tacgcacggt cccgcgcgac gcgacgctct accccgagct
      661 gaccccgcag atccgcgacg cgatgctcgg cgagacccac cgcttcgtca gcagcatcta
      721 ccgcgagggc ggcaccctgg ccgacctgct gaccgcctcg tactcgctga tgaccgacgc
      781 gctcgcgcaa tactacgacc tgcctcccgg ggcaggtgag cccgacgccg ccggttacaa
      841 gcgcaccgac accgccggcg cggccggcct gctggcccac ggtggcctgc tgacgaccca
      901 cgcgctgccg accagctcct cgccgatcca ccgcggcaag ctggtgcgcg agcgcctgct
      961 gtgtcaggac atgccgccgc cgcccccggc cctcgacacc tcgccgccgc cggtcgaccc
     1021 gagcctgagc acgcgcgagc gctacaccca gcactcgtcg gacccgacct gcgccggctg
     1081 tcacacgcgc atcgacccga tcggcttcgg cttcgagcac tacgacgcga tcggccgctt
     1141 tcgcctcaag gacggcgtgc acgacgtcga tgccagcggc gagatcgtgc tgaccgacca
     1201 gaccaacggc accttcgacg gcatcgacga gctcgcggcg ctgctcgccg gcagccccga
     1261 ggtcgaggcc tgttacacgc tgcagtggtc gcgccacgcg ctcgggtccg ccgaggccag
     1321 cgagctcgcg tgcgtccagg acgaggtcac cgccgccttc accggcgccg agggccgcct
     1381 cgacagcctc gtgctcgcgc tcgtcggcac ccgcttcttc cgcgagcgcg gcggcgccgt
     1441 caccgacgac cccgggtccg agaccggcag cgacaccggt agcgacaccg gcagcgacga
     1501 caccgggacc actaccacga gcggcggcga caccggcagc gacaccggcg accagcccgg
     1561 cccgatgacg acgcccggca tcgaggccgt gctcgtccag gactccgtgt gggccgccgg
     1621 cgagtgcgac accgtcaccg tcaccaacac gaccgacgcc ccgatcacct ggcaagccgg
     1681 cgtcgccctg aagggcacga tccagaactt ctggaacgcc aagtatgtcg ccgtcgacgg
     1741 cggcttcgtg ttcaccggcg aggcctacaa cgccgtggtc gcccccaaga cctcgaccag
     1801 cttcggcttt tgcctgaact attgaaattg cgcatgcacc tgccccttcg gacagccctc
     1861 gccgcgagca ccctgctcgc gtgcgcttcc tgatcgatca gacccagggc gagctgatca
     1921 cccgggtcga cggcctcgag ttcgcaggct tgcacgtcga cggcgatccg accccggatg
     1981 tcggcctgtc ctcgaggaca tcaagttcgg ctgggagagc tacgccggcc agacgatggt
     2041 cctgtacttc gacgacatcg cgctcgccga cgccccgatc ggctgcgact gacacacgcg
     2101 ctggatctct ggtagcgtcg cggtgcttgc cgatccgcgt ccgccaccgc ctcaccggcc
     2161 tgcatctggt ggcgctcgcg ctgaccctgc cggcccctgc gctcgccgcc ccctgcccga
     2221 cgatcgccgg aggcagcgag gccctgacgg agcacgactc cgaggcccgc ctcgccttca
     2281 tccgcgcgag gatgaaacac gacgcctggc gcgcccgcca gtggagcctc ggctacggca
     2341 ccggctacgc gctgctcacc ggcgcctccg cgatcgccgc cccgctgatc aaggaccgcg
     2401 cctcggtccc ggacctctat gtcggcggct tctcgtcgat catcggcttc ggcctgatcg
     2461 ccgtgtcccc attaaaagtg atcttcgacc gcgaggagct cgaggcccag atcgaaaagg
     2521 cgggccccgg cggcgatcgc tgcgccctgc tggcgaccgc cgaggcgatg ctgatcgccg
     2581 acgcgaagaa cgagcgcttc ggtcgctcgt ggctgatcca cagcggcaac gtgctcgtcg
     2641 gcgtcggcgc cctgctggtg ctcggcctcg gctacgaccg ctgggggtcc ggcatcgcca
     2701 acggcatcgc cagcatcgcc gtcggcgagg tcatgatctt cacccagccg gtcggcctcg
     2761 tccgcgacct caaacgctac cgcctcggtg acctcagcca accgacccgc cgccgcaaac
     2821 gcatcgcctg gggcggcgcc ccgatggtca ccccgccggg gaccggcgct ggcgtgtatg
     2881 gattatcgtt gatgggccgc ttctaggtgc cacccctccg ccagcctggc gcagtgggtc
     2941 gaccaggcca gtcgcaaggg taggcgtgac ttcgcccgtc accgggtcta tcatcgcgcg
     3001 atgcgtcccg tccgcctcgc ggtctgcatc cttgctgtcg ccctcgcgtg cggcggcggc
     3061 tccggtggtg gcttcgcgtc gagcatgggc ccggcgatca cgtccgtatc cgcagggacc
     3121 gacgccggct cgacctccac gacggccagc tcgacctccg gggacataag ctcgacctcg
     3181 ggcgaagcaa gctcgacctc ggcggcgagc gccgccagta cgggcacgat ctacgacctc
     3241 ggcggcggcg gtgacatcgg acccatcgcc ccaccaggct gcaaggacag gatcgacttc
     3301 ctgttcgtga tgaagcgagg tttacagctg cccgagcacc agcagaagct cctcgccgcc
     3361 ttcccggcct tcatcgacac catccaggac aagttcgccg ggttcgacgt ccacatcatg
     3421 gtcgtcgaca ccgagtcgtg gtggggacaa caatcctgcg atcccgaggg ctgcctggac
     3481 accgacaaca acggctgcca gatcggtgag catttcgtcg cggactaccc ctgcggtgta
     3541 aaggcgacgc tcggcaagtg cgacaagacc ctcggcgccg gcaccgtctt caacgccggg
     3601 ctcgactccg cgaacgtgcc ctgcaagctc gacggtggcc accgctacat cacccaggac
     3661 cagaccgacc tcaaggccac cttcgcctgc atggcccagg tcggccaggg tgggggcgaa
     3721 aatgtcggcc aggccaccgc cgaggccgtc agcccggcgc tcaaccagga cggtggatgc
     3781 aacgctggat ttttgcggga cgacgccctg ctcatgatca ccttcgtcgc acccggcggc
     3841 gatgacgatt cggacggaac gccgcaggac tgggccgacg ccgtgtttgc cgccaagcac
     3901 ggcgacaaga acgccgtcgt catgttgggc atccacggcg tggctcccga cggcggccgc
     3961 ggccgactcg tcgagtttct cgagctgttt ccgttctcgt tgcagggcta cctgaccgag
     4021 cccagcttcg ggcccatctt cgccgacgcc gtcgagatgg tcgacgcggc gtgccagggc
     4081 tttaccccac cggggtaatt ggtcgccggt ctcgttattc gtcagccgtg gccgcgaccg
     4141 gcgaccgcgt gcaggacgcc gccgatgctc cgcacgccac cgaaccagat gtagccgagc
     4201 ggcccgaccg ccacagcctg ccccatctcg agcccgggag tgtcgaacaa gtaactcagc
     4261 gcgaggtcga cgtcgtcggt gcggtgcccg gtgacccgca gcgcgtagtt gaggcccggt
     4321 cccatgctca ccgccgacac gacgacgttg tat
//
LOCUS       DASWNJ010001093         4003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_124825, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001093 DASWNJ010000000
VERSION     DASWNJ010001093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124825"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3972)
                     /locus_tag="VGB85_18390"
     CDS             complement(<1..3972)
                     /locus_tag="VGB85_18390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136065.1"
                     /translation="MRAAEGAWSSGQVDGEVDPLAATRVALRGDVATRRGAMQAEIEG
                     VPAFVLARARALALRFVDRAGCLEAVTVWAREAPADPEPAWLRVVQPAMRGDLEGVTA
                     ALAELAERPGPAVVTMQRRLALALALQRSEAGAAQARALLEPMVAGLGEAPAGLQAGV
                     WRALARARAGDAAAPGGVVEDAVAAALGDEGWRRRGETGELRAQVAAALVGSGRAEAD
                     TTRLLRRMLGDERPARTVRSTGVTRPARRRQEDRGTGARIVADYYAQEGRWSELITLL
                     GRELVQLDGVARIQALRRIARIHRHYLHDPASAEQALRVALAQPAEDEAILHDRLLLH
                     GELVTCLEMQGRFAEAVAHLEAALQDELSAGTGEEAEPGPERVELLVRLARLARDGLD
                     DEARAAPAFAALVRAGAAPDDGLATLTRVYHGTGDYPRLAEVLRLRLARVDPEREVER
                     AAELHRRLADLLDGPLAKGSEAAAHHLAAYLADPQAHEVSRARARALLAGANVSAAVR
                     HATVAGVLAELPEEPLREVQAWTLAAEAAAAAGGVEEAEALFRAALASAAVDLSAGTG
                     LSDGGHLAEGTGGVGEGAGAWLGAGGGSHLAEGTGTGGVGEGAGAWLGAPEDDVASAA
                     IGAAAGGLGRVLLGLGRGEEATRALAFAAGRRGLADAAAIEHALLAAGAAIAGGRVAE
                     AEAVLVAVRGARAVDERVLLELARAYALQGRVDAADAVLGELAQANAPTLRAEALLRR
                     AKLRDFMADARGGVGGEALALLQAAFAADPRNAEVREVLRSLAAARGAWPIVVQAIMA
                     ALRGLPAGAERVALLLDLGEVHLLRLHDPDSAARSVERAIALAPNDALVRRRIVGLLA
                     DVREDMSHAPGLDGHAWLRGLAGSQELGDAGAACVWLTLGESCLRAQDVDGAAGAWQQ
                     VFALANPGPAALAEARQHLATLGAGGDLQAQRVALQRLLAGEDQPEERLPILARLWEI
                     GEALGDDELVEASCREALALAHDDPGQDELRAAAASGLRGVLTRRGTTGEIARLYAGL
                     DADTQDASQAARALVDAARFAGQVLHDAKLAASLAHRAWRRAPADAAAQAMLAEVAGH
                     ATHGPALLDALAEESLEMAPELALRLADAAVVLARPALARRWLAALAEVEDLEVRRAA
                     LDRLDALLVGAKAERLPVLRARVRAHGSREVGDMPDRTGAPGSGDTSDRAGLAGPGDM
                     LALELARLEVELGELDAALATCLPGPIAEATDAALLELAGELLARREQWAEVTAVCER
                     RAELAAQAGDGAGESSWLTRAAQVCIDHATHPRRAMQDARRLLLRACAADAAGEAARA
                     LLVP"
BASE COUNT          448 a         1838 c         1301 g          416 t
ORIGIN      
        1 cggcaccagc agggcccgcg ccgcctcgcc cgcggcatcg gccgcgcacg cgcgcagcag
       61 caggcgccgc gcgtcctgca tcgcccgccg cgggtgcgtc gcgtggtcga tgcagacctg
      121 cgccgcgcgc gtcagccacg acgactcgcc cgccccgtca ccggcctgcg ccgccagctc
      181 ggcccgccgc tcgcacacgg cggtcacctc ggcccactgc tcgcgccgcg ccagcaattc
      241 acccgccagc tcgagcagcg ccgcgtccgt cgcctccgcg atcggccccg gcagacaggt
      301 cgccagcgcc gcgtcgagct ccccgagctc gacctcgagg cgcgccagct cgagcgccaa
      361 catgtccccg gggccagcca agcctgcccg atcggacgtg tccccggagc caggcgctcc
      421 tgtccgatcg ggcatgtccc cgacctcgcg cgacccgtgg gcccgcaccc gcgcgcgcag
      481 caccggcagt cgctccgcct tcgcgccgac gagcagcgca tcgagccgat cgagcgcggc
      541 cctgcgcacc tcgaggtcct cgacctccgc cagcgccgcg agccagcgcc gggccagcgc
      601 cgggcgggcc agcacgaccg cagcgtccgc gagccgcagc gccagctccg gcgccatttc
      661 aaggctctcc tcggccagcg cgtcgagcag cgccggcccg tgcgtcgcgt gcccggcgac
      721 ctccgcgagc atcgcctgcg ccgccgcgtc cgcgggtgcg cgtcgccagg cgcggtgcgc
      781 cagcgaggcc gcgagcttcg cgtcgtgcag cacttgcccg gcgaagcggg ccgcgtcgac
      841 cagcgcgcgc gcggcctggc tggcgtcctg cgtatccgcg tcgagcccgg cgtacagcct
      901 ggcgatctcc ccggtggtcc cgcggcgggt cagcaccccg cgcagcccgc tcgccgccgc
      961 cgcgcgcagc tcgtcctgcc cggggtcgtc gtgcgccagc gcgagcgcct cacggcagct
     1021 cgcctcgacc agctcgtcat caccgagcgc ctcgccgatc tcccacagcc gcgcgaggat
     1081 cggcaggcgc tcctcgggct ggtcctcgcc cgcgagcagg cgctgcagcg cgacgcgctg
     1141 ggcctgcaag tcaccgcccg cgccgagcgt cgccaggtgc tgccgcgcct ccgccagcgc
     1201 ggccggcccg gggttggcga gcgcgaacac ctgctgccac gcgcccgccg ccccgtcgac
     1261 gtcctgcgcg cgcaggcacg actcgccgag cgtcagccac acgcacgccg cccccgcgtc
     1321 gccgagctcc tgcgagcccg ccagcccgcg cagccacgcg tgcccgtcca accctggcgc
     1381 atgggacatg tcctcacgga catccgcgag cagcccgacg atccgccggc gcaccagcgc
     1441 gtcgttcggc gccagcgcga tcgcccgctc gacgctgcgg gccgcgctgt cggggtcgtg
     1501 caggcgcaac aggtggacct cgccgaggtc gagcagcagc gcgacccgct ccgcccccgc
     1561 cggcagcccg cgcagcgccg ccatgatcgc ctggaccacg atcggccagg ccccccgcgc
     1621 cgccgccagc gagcgcagca cctcgcgcac ctcggcgttt cgcgggtccg cggcgaacgc
     1681 cgcctgcaac agcgccagcg cctctccgcc gacgccgccc cgcgcatcgg ccatgaagtc
     1741 ccgcagcttc gcgcgccgca gcagcgcctc ggcccgcagc gtcggcgcat tcgcctgcgc
     1801 gagctcgccg agcaccgcgt cggccgcgtc gacccgcccc tgcagcgcat acgcgcgcgc
     1861 cagctcgagc agcacccgct cgtcgaccgc ccgcgccccg cgaaccgcga cgagcaccgc
     1921 ctccgcctcc gccacgcgcc cgccggcgat cgcagccccg gcggccagca acgcgtgctc
     1981 gatcgcggcc gcgtccgcga gcccgcgccg gcccgccgcg aacgccagcg cccgcgtcgc
     2041 ctcctcgccg cgaccgagcc cgagcagcac ccgcccgagc ccccccgccg ccgcgccgat
     2101 cgccgccgac gcgacatcgt cctcgggcgc gccgagccac gcccccgccc cctcgcccac
     2161 gccgccggtg cctgtccctt cagccaggtg actcccgcca cccgcgccga gccacgcccc
     2221 tgccccctcg ccgacgccgc ctgtcccttc agccaggtga cccccgtcgc tcagacctgt
     2281 ccctgcggac aggtcgaccg ccgcgctcgc gagcgccgcg cggaacaggg cctcggcctc
     2341 ctcgaccccg cccgccgccg ccgccgcctc ggccgcgagc gtccacgcct ggacctcgcg
     2401 cagcggctcc tccggcagct ccgcgagcac acccgccacc gtcgcgtggc ggaccgccgc
     2461 cgacacgttc gccccggcga gcagcgcccg cgcccgcgcc cggctcacct cgtgcgcctg
     2521 cgggtcggcg aggtacgccg cgaggtgatg cgccgccgcc tcgctgccct ttgcgagcgg
     2581 cccatcgagc aggtccgcga gtcgccggtg cagctccgcc gcgcgctcga cctcccgctc
     2641 cgggtcgacc cgcgcgaggc gcaggcgcag cacctcggcc agccgcgggt agtcgccggt
     2701 gccgtggtac acgcgggtca gcgtcgccag cccgtcgtcg ggcgctgccc cggcgcgcac
     2761 cagcgccgcg aaggccggcg ccgcgcgggc ctcgtcgtcg agcccgtcgc gcgcgagccg
     2821 ggccaggcgc accagcagct ccacgcgctc gggccccggc tccgcctcct cacctgtccc
     2881 tgcggacagc tcgtcctgca acgccgcctc gaggtgcgcg accgcctcgg cgaagcgccc
     2941 ctgcatctcg aggcaggtca ccagctcgcc gtgcagcagc agccggtcgt gaaggatcgc
     3001 ctcgtcctcg gccggctggg ccagcgcgac ccgcagcgcc tgctccgcgc tcgccgggtc
     3061 gtgcaggtaa tgccggtgga tcctggcgat ccttcgcagc gcctggatcc tcgccacccc
     3121 gtcgagctgg accagctcgc ggccgagcag cgtgatcagc tccgaccagc ggccttcctg
     3181 tgcgtaataa tcggcgacga tccgcgcgcc ggtcccccgg tcctcctgcc gcctgcgcgc
     3241 cggtcgtgtc acccctgtcg acctgaccgt ccgggcgggt cgctcgtcgc cgagcatgcg
     3301 ccgcagcagc cgcgtcgtat cggcctccgc ccgcccgctg ccgaccagcg ccgccgcgac
     3361 ctgggcccgc agctcccccg tctcgccgcg cctgcgccag ccctcgtccc cgagcgccgc
     3421 cgcgaccgcg tcctcgacca ccccaccggg cgcagccgcg tcgcccgccc gcgcgcgcgc
     3481 cagcgcccgc cacacgccag cctgcaaccc ggcaggcgcc tcgccgagcc ccgcgaccat
     3541 cggctcgagc agcgcccgcg cctgcgcggc cccggcctcg ctgcgttgca gcgccagcgc
     3601 cagcgccagc cggcgttgca tcgtcacgac cgccggcccc ggccgctccg ccagctccgc
     3661 cagcgccgcg gtcacgccct ctagatcccc gcgcatcgcc ggctggacga cccgcaacca
     3721 cgccggctcc gggtccgcgg gcgcctcacg ggcccacacc gtgaccgcct cgaggcagcc
     3781 cgcgcgatcg acgaagcgca gcgccagcgc ccgcgcccgc gccagcacga acgccgggac
     3841 cccttcgatc tcggcctgca tcgcgccgcg ccgcgtcgcc acatccccgc gcagcgcgac
     3901 ccgcgtcgcc gccagcggat cgacctcgcc gtcgacctgt cccgaagacc aggccccttc
     3961 cgccgcacgc acgcgcaccc cctcgctccc gacctcgacc acc
//
LOCUS       DASWNJ010001094         2343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_585059, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001094 DASWNJ010000000
VERSION     DASWNJ010001094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585059"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VGB85_18395"
     CDS             complement(<1..663)
                     /locus_tag="VGB85_18395"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY0136066.1"
                     /translation="MPTLPDALASYVPVLITRRLAADPRPLVEPAAELRAAAVLCADI
                     SGFTAFTEMLARQGPAGVEKVGSGLDGYFSRLTAIIEAHGGDVVKFAGDALTAIWLPP
                     DGSEDLQAAALPAIACGLAIQDSLHGYRVSDGVELSLRVGIGASRIAAVQIGGLAGRW
                     ELALTGAALADAWKAEGLAKPGEVVVAPRVWSLIDRHVRGRPAGDGSVHVEALAAAFF
                     CAP"
     gene            934..2196
                     /locus_tag="VGB85_18400"
     CDS             934..2196
                     /locus_tag="VGB85_18400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136067.1"
                     /translation="MIGDLCATARGCGLLAACGTLALAIAACASGDPGVDSANPFTTA
                     RPVDDTGTPVTEASSPTDGSATGEGPGGNSDSNSDTGSSVTNVTTAETGTTEPVDPTE
                     NVDPDCIDDDDDGFGENCDLGPDCDDADYNNHTAEGCANCVDNDGDDVWSGCDQYGEQ
                     APGPDCDDANPQVGAGDAVELCNGIAENCAGEIDPLAPEDMCPTTGEAPNVAPVGGWK
                     CNMVPPGQDGCEIATCMPGFFDANKTPADGCECVGTDRNKSLDACSDDPKGAQGPVAE
                     GGVLPPIKGVIPFVDNGPGMGMEDWYSVTFPEAMAMGARPNTGNVVVTFAINPGDPAN
                     PDYRLEVYRSCGGPAFEGGLATQFGAGAPPAREWTFVDTPNPGNPMPNPNAKNDVPWP
                     EKVFVRVIRVNNSGTCGEYSIQISRLPN"
BASE COUNT          394 a          945 c          740 g          264 t
ORIGIN      
        1 cggcgcgcag aagaacgcgg ccgccagcgc ctcgacgtgc acggagccgt cgcccgccgg
       61 ccgcccgcgc acgtgccggt cgatcaacga ccacacgcgc ggcgcgacga ccacctcgcc
      121 cggcttcgcc agaccctcgg ccttccacgc gtccgccagc gccgcgccgg tcagcgccag
      181 ctcccagcgc cccgcgagcc cgccgatctg caccgccgcg atccggctcg cgccgatccc
      241 gacgcgcagc gacagctcga ccccgtccga cacgcgatat ccgtgcaggc tgtcctggat
      301 cgccagcccg cacgcgatcg ccggcaacgc ggccgcctgc aggtcctccg agccgtccgg
      361 cggcaaccag atcgccgtca gggcgtcgcc cgcgaacttg acgacgtcgc cgccgtgggc
      421 ctcgatgatc gcggtcagcc gactgaaata accatcgagc cccgagccga ccttctcgac
      481 ccccgccggc ccctgccgcg cgagcatctc ggtgaacgcc gtgaacccgg agatgtccgc
      541 acagagcacc gccgccgccc gcagctcggc cgccggctcc accagcggcc gtgggtcggc
      601 cgccagccga cgggtgatga gcaccgggac gtagctggcg agggcgtcag gaagcgtcgg
      661 caccacgcgc ggcgatggta ccccagccgc gacccgggcg atagcgcagc gccgactcga
      721 accccttggt ccggccgcgg ggacccggcg gcccaccccc gccgcgcctc gccgatatcc
      781 cgcggtcgaa agccaggccc gcggccccgg aacccgcgac cgatccacac cggccgaccc
      841 tgagagcctg gtggcgcgga cctgtgtcct cgcgcacgat cgagcgacgt cggtccctgc
      901 acgccgtggt tgcgcctcgt gtatggtcgc caaatgatcg gtgacttgtg tgcaacggca
      961 cgtggctgcg gtctgctggc ggcctgcggc accctcgcgc tggcgatcgc ggcctgcgcg
     1021 agcggcgacc ccggggtcga ctccgccaac ccgttcacca ccgcccgccc ggtcgacgac
     1081 accggcaccc cggtgaccga ggcctcctcc ccgaccgacg gatccgccac cggcgagggc
     1141 ccgggcggca acagcgacag caacagcgac accggcagca gcgtcaccaa cgtgaccacc
     1201 gccgagaccg gcaccaccga gcccgtcgac ccgaccgaga acgtcgaccc cgactgcatc
     1261 gacgacgacg acgacggctt cggcgagaac tgtgacctcg gccccgactg cgacgacgcc
     1321 gactacaaca accatacggc cgagggctgc gccaactgcg tcgacaacga cggcgacgac
     1381 gtgtggtccg gctgcgacca gtacggcgag caggcccccg gccccgactg cgacgacgcc
     1441 aacccccagg tcggggccgg cgacgcggtc gagctgtgca acggcatcgc cgaaaattgc
     1501 gcgggagaga tcgacccgct cgcacccgag gacatgtgcc cgaccaccgg cgaggcgccc
     1561 aacgtcgccc ccgtcggcgg ctggaagtgc aacatggtcc ccccgggcca ggacggctgc
     1621 gagatcgcca cctgcatgcc cggcttcttc gacgccaaca agacccccgc cgacggctgc
     1681 gagtgcgtcg gcaccgaccg caacaagtcc ctcgacgcct gcagcgacga ccccaagggc
     1741 gcccagggcc ccgtcgccga gggcggcgtg ctcccaccga tcaaaggcgt cattcccttc
     1801 gtcgacaacg gccccggcat gggcatggaa gactggtact cggtcacctt ccccgaggcg
     1861 atggcgatgg gcgcccgccc caacaccggc aacgtcgtcg tcaccttcgc gatcaacccc
     1921 ggcgacccgg ccaacccgga ctaccgcctc gaggtctacc gcagctgcgg cggcccggcc
     1981 ttcgagggcg gcctcgccac ccagttcggc gccggtgccc cacccgcccg cgagtggacc
     2041 ttcgtcgaca cgcccaaccc cggcaacccc atgcccaacc ccaacgccaa gaacgacgtg
     2101 ccgtggcctg aaaaggtctt cgtcagggtg atccgggtca acaacagcgg gacttgcggc
     2161 gagtactcga tccagatctc gcggctgccg aactgactcc gacactctac cgcagcagtc
     2221 gatcgtagcg aggcgatcaa cgcgtcacag cgggaggcgg agctgtcgcg ctggcgcggc
     2281 gagctctttg cgtccacggc tcgcaacgca cgcccattct tgatacagcc gaaacgctgt
     2341 aca
//
LOCUS       DASWNJ010001095         2730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_478635, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001095 DASWNJ010000000
VERSION     DASWNJ010001095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478635"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VGB85_18405"
     CDS             <1..415
                     /locus_tag="VGB85_18405"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0136068.1"
                     /translation="KETLTRSVPPATPNIKAKGKSLRLKKSIRYDGNNPDAKTQPLLD
                     ELAAFLRAHPEYEELEIGVHSDDRGNPKQKTTERADNLRNYLLSKGVGPERIKAVGFG
                     ASKPVAVNMTASGRAKNNRTVLRVTKYTKPAAAPK"
     gene            572..2167
                     /locus_tag="VGB85_18410"
     CDS             572..2167
                     /locus_tag="VGB85_18410"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0136069.1"
                     /translation="MPLSWIALLSLTVGCADDAPANATATAITSSATGSSGDDPTTGA
                     PGSTGASAATTGDESTSPTSPATSTSPTSSATATSLTDGSSTDEGETGAPGETSDTDP
                     GATDTTDTGDTEDTDTDGPLLPGCGPTPPDDFMAQPSAPGGGATVPGPAVSLEVTIGG
                     TILADATANFHLREVHALTDADDFTVVILPDTQNYTQFPGNFPQYPAQTKWVWEHRNT
                     DNIVGLMHNGDVVQHGNMVEEEWVRAEKAMVTLETSTEKFIDGMPYSIAVGNHDNGAY
                     AHDDKPFSTSMFNKYFGVSRFKNRKYYGGHYGDKNDNSWILVQAGNLEVLMLSLEYRG
                     DLGQDPKVLAWAKGVFEAHPDALGIVNSHHIVKANGDFSVEGKQIYNVMKTVKNIQLM
                     TSGHIKEFRKRRTDEYQGRKIHSMLADYQDLIVPADDCAGGCGYMRIWRFSPKKNELR
                     VQTYSPPLDKFMTAAQEQFTLTVDLSAATGVFALQGATSGPGKTLAYTVDGLKAGATY
                     EWYAVVKECGAKVTTPKRKFTVN"
     gene            complement(2285..>2730)
                     /locus_tag="VGB85_18415"
     CDS             complement(2285..>2730)
                     /locus_tag="VGB85_18415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136070.1"
                     /translation="FYDGQVQTDSGTVVAFAPTAADPNAGRARQEPAAPDFAAQEPMR
                     SFGSRLRGADDAAPSAPSLSPESAPSVSAEIPSFAGNNLNVAVGPVAPVTPATPATAP
                     RPEVQPDELDQLDTASGSNTKLIVIGLVVVVAIVVAIIMATGGNN"
BASE COUNT          508 a          963 c          912 g          347 t
ORIGIN      
        1 caaggagacc ctgacgcgct cggtgccgcc ggcgaccccg aacatcaagg ccaagggcaa
       61 gtcgctgcgg ctcaagaagt cgatccgcta cgacggcaac aacccggacg ccaagacgca
      121 gcccttgctc gacgagctgg ccgcgttcct gcgcgcccac cccgagtacg aggagctcga
      181 gatcggcgtg cacagcgacg accgcggcaa ccccaagcag aagaccaccg agcgcgccga
      241 caacctccgc aactacctgc tgagcaaggg cgtcggcccc gagcgcatca aggcggtcgg
      301 cttcggcgcg agcaagccgg tcgccgtcaa catgaccgcc tcgggccgcg cgaagaacaa
      361 ccgcaccgtg ctgcgggtga ccaagtacac caagccggcc gccgcgccga agtagcgtcc
      421 gcgcctgcgg gcttcgagtt ccgcgctggc ggcctgtccc gagggacagg ccgccgttcg
      481 tttgtcggcc gcgtcactgc gagcgggggc ggcccgtttg gcgccgtcca cgcgaaggcg
      541 ttgactctat actgcacgga tgcgcgcagc gatgcccctg tcctggatcg ccctcctgtc
      601 cctgaccgtc ggctgcgccg atgatgcgcc cgcgaacgcc accgcgacgg cgatcaccag
      661 cagcgcgacc ggcagcagcg gcgacgatcc gaccacgggc gccccgggca gcaccggggc
      721 cagcgcggcc accacaggcg acgagagcac gtcccccacc agcccggcca cgagcacttc
      781 cccgacgagt tcggcgaccg ccacctcgct caccgacggc agcagcacag acgagggtga
      841 gaccggcgcg cccggcgaga ccagcgacac ggaccccggc gccacggaca ccaccgatac
      901 cggtgacacg gaggacaccg acaccgacgg cccgctgctg cccggctgcg gcccgacgcc
      961 gccggacgac ttcatggccc agccgagcgc gccgggcggt ggcgcgacgg tacccgggcc
     1021 ggcggtcagc ctcgaggtca cgatcggcgg cacgatcctc gccgacgcga ccgccaactt
     1081 tcacctgcgc gaggtgcatg cgctgaccga cgccgacgac ttcacggtcg tcatcttgcc
     1141 ggacacgcag aactacacgc agttcccggg caacttcccc cagtacccgg cgcagaccaa
     1201 gtgggtgtgg gagcaccgca acaccgacaa catcgtcggg ctgatgcata acggcgacgt
     1261 cgtccagcac ggcaacatgg tcgaggagga gtgggtgcgc gccgagaagg cgatggtcac
     1321 cctcgagacc tcgacggaga agttcatcga cgggatgccc tacagcatcg cggtcggcaa
     1381 ccatgacaac ggcgcctacg cgcacgacga caagccgttt agcacgagca tgttcaacaa
     1441 gtacttcggc gtatcgcggt tcaagaaccg caagtactac ggcggccact acggcgacaa
     1501 gaacgacaac agctggatcc tcgtgcaggc gggcaacctc gaggtgctga tgctcagcct
     1561 cgagtaccgc ggtgacctcg gccaggaccc gaaggtgctc gcgtgggcca agggcgtgtt
     1621 cgaggcgcac cccgacgcgc tgggcatcgt caactcgcac cacatcgtca aggccaacgg
     1681 cgacttcagc gtcgagggca agcagatcta caacgtgatg aagacggtca agaacatcca
     1741 gctgatgacg agcgggcaca tcaaggagtt tcgcaagcgt cgcaccgacg agtaccaggg
     1801 ccgcaagatc cactcgatgc tcgccgacta tcaggacctg atcgtcccgg cggacgactg
     1861 cgccggtggc tgcggctaca tgcggatctg gcggttctcg ccgaagaaga acgagctgcg
     1921 ggtccagacc tactcgccgc cgctcgacaa gttcatgacc gcggcccagg agcagttcac
     1981 cctcaccgtc gacctctcgg ccgccaccgg ggtcttcgcg ctccagggcg ccaccagcgg
     2041 cccgggcaag accctcgcgt acaccgtcga cggcctcaag gccggcgcga cctacgagtg
     2101 gtacgcggtc gtcaaggagt gcggcgcgaa ggtgacgacg cccaagcgca agttcaccgt
     2161 caactgagcg gcgccgcgtg catgtcccca aggacatatg tgcgcgcaac caatgaaaaa
     2221 cgccgcccgg tcgacccgga cggcgtctct catgcgctgg cgcttaccag cgcttaccag
     2281 cgccttagtt gttgccgccg gtcgccatga tgatggcgac gacgatcgcc acgaccacga
     2341 cgaggccgat gacgatcagc ttggtgttgc tgccgctggc agtgtcgagc tgatcgagct
     2401 cgtccggctg cacctcgggg cgcggggcgg tcgccggggt cgccggggtc acgggagcga
     2461 ccgggccgac ggcgacgttg aggttgttgc cggcgaagct cgggatctcg gcggacaccg
     2521 agggggcgct ctcgggggac agcgagggcg cggacggggc cgcatcgtcg gcgccacgca
     2581 gccgggatcc gaacgatcgc atcggctcct gcgcggcgaa gtcgggcgca gcgggctcct
     2641 ggcgggcgcg tccggcgttg ggatccgccg cggtcggtgc gaacgcgacg accgtgccgc
     2701 tgtcggtctg gacctggccg tcgtagaact
//
LOCUS       DASWNJ010001096         2197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_231345, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001096 DASWNJ010000000
VERSION     DASWNJ010001096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231345"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /locus_tag="VGB85_18420"
     CDS             <1..1504
                     /locus_tag="VGB85_18420"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEY0136071.1"
                     /translation="QKPGAWNEHLVPFMADGDGQLYCIDRTGSIDGGTPGQVVFYDHE
                     TGAGREFTSFEVMLDLLTTLAKKNLLDQEAREEDEELQEKYDDLYASAKNVGMPKMPA
                     KQLKKTFETIEELTRARAQLEMALPLARQYPAEADLWCLVVHAASELGDWPLVAEAAT
                     TAERLTPARDRPRMATSLATALHKLGRDDEALAVVATAISGNAMSNYAASKIPTDVSP
                     EFRHRCLQHAVELRPRDFDLWLMFGSEAPTPGERAAALEHTITLCSDAKLIRYNEDLA
                     KDAIRKARVVLELDRVEALSGAAKLKGLLALAKRVPTEESRPLWERTIACAVAQGDWK
                     VVSAAGEGLVAQGPDSKRHLACPWQVLALHRLGREAKALASLREALAAMYDEESAEAL
                     RAVPWNEHGGPHHVERAPGDEQFEASCYVLATEVQPENAFVWSERARTAQTSAERRAA
                     LARVVALCTPKRVDFVEDRKLAEVYNEANRKKSAALRRIRDEAIAQLAQA"
     gene            complement(1626..>2197)
                     /locus_tag="VGB85_18425"
     CDS             complement(1626..>2197)
                     /locus_tag="VGB85_18425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136072.1"
                     /translation="DPTRPPDLYERRYMAVEGAVLYRHKHSAPWQLHALFFAATVAAF
                     GSAFAVGGVVGGAVGVAVGVPLLALAWLVSAVLRVSVSEQHVNIQYGLFGPKIPIADI
                     EQAELVQYDWRRIGRWSLKRSLAGELVINMPGDGGRAVRMTWRDARGKRRVVHVGSPR
                     ADDLLRQIGRAQRALTAGPRRASLEPGGP"
BASE COUNT          376 a          742 c          784 g          295 t
ORIGIN      
        1 acaaaagccc ggggcctgga acgagcacct ggtgccgttc atggccgacg gcgacggcca
       61 gctgtactgc atcgaccgca ccggttcgat cgacggcggc acgcccggcc aggtcgtgtt
      121 ctacgaccac gagacggggg ccgggcgcga gttcacctcg ttcgaggtca tgctcgatct
      181 gctgacgacg ctcgccaaga agaacctgct cgaccaggag gcccgcgagg aagacgagga
      241 gctgcaggaa aagtacgacg atctgtatgc cagcgccaag aacgtcggca tgcccaagat
      301 gccggccaag cagctcaaga agacgttcga gacgatcgag gagctgacgc gggcccgggc
      361 gcagctcgag atggccctgc cgctcgcgcg gcagtacccc gcagaggccg atctatggtg
      421 cctggtggtt cacgccgcct cggagctcgg cgactggccg ctcgtggccg aggccgcgac
      481 cacggccgag cgtctgacgc ccgcgaggga tcgtccgcgc atggcgacat ccctcgccac
      541 tgcgctgcac aagctcggcc gtgacgacga ggcgctggcg gtggtggcca cggcgatctc
      601 gggcaacgcg atgagcaact acgccgcgag caagatcccc accgacgtca gcccggagtt
      661 tcgccatcgc tgcttgcagc acgccgtcga gctccgaccg cgagacttcg atctctggct
      721 gatgttcggc tcggaggcgc ccaccccggg agagcgcgcg gctgcgctcg aacacacgat
      781 cacgctgtgc agcgacgcga agctgatccg gtacaacgag gatctggcca aggacgcgat
      841 ccgcaaggcg cgcgtggtgc tcgagctcga ccgcgtcgag gcgctctccg gagcggcgaa
      901 gctcaaaggc ctgctcgccc tggcgaagcg ggtcccgacg gaggagtcca ggccgctgtg
      961 ggagcgcacg atcgcgtgcg ccgtggccca gggcgactgg aaggtggtca gcgccgcggg
     1021 cgaagggctg gtcgcgcagg gcccggatag taagcgtcac ctcgcctgcc cgtggcaggt
     1081 gctcgcgctg caccgactcg ggcgcgaggc caaggcgctg gcgtcgctgc gcgaggcgct
     1141 cgcggcgatg tacgacgagg agtccgcgga ggccctgcgc gcagtgccgt ggaacgaaca
     1201 tggtgggccc catcacgtcg agcgtgcgcc aggcgacgag cagttcgagg ccagctgtta
     1261 tgtgctggcg accgaggttc aacccgagaa tgcgttcgtc tggagcgagc gtgcacgcac
     1321 cgcgcagacc tcggccgaac gcagggccgc gctcgcgcgg gtcgtcgcgc tgtgcacccc
     1381 gaagcgcgtc gatttcgtcg aggaccgcaa attagccgag gtctacaacg aggcgaaccg
     1441 caagaagagc gcggctctgc gccgcattcg ggacgaggcg atcgcccagc tcgcgcaggc
     1501 gtgatcatgc gtgatcatgc gtgatcatgc ctgatcatgc ctgatcatgc ctgatcatgc
     1561 ccgaccgtcg cgcccggttc gcgcgatggt tcgaggctga tacgatgcgg ccgtcatcga
     1621 cgacatcacg gaccgccggg ctcgagcgaa gcgcggcggg gcccggcggt cagggctcgc
     1681 tgcgcgcggc cgatctggcg cagcagatcg tcggcccgcg ggctgccgac gtgcacgacg
     1741 cggcgcttgc cacgagcgtc gcgccaggtc attcggaccg cgcgaccgcc gtcgcccggc
     1801 atgttgatga ccagctcacc cgcgagcgag cgcttgaggc tccagcggcc gatgcgacgc
     1861 cagtcgtact ggacgagctc ggcctgctcg atgtcggcga tcgggatctt ggggccgaac
     1921 aggccgtact ggatattgac gtgctgctcc gagacgctga cgcgcaggac cgcggacacg
     1981 agccacgcca gcgcgagcaa cggcacgccg acggcgaccc cgaccgcacc gccgaccacg
     2041 ccgccgaccg caaacgccga gccgaacgcc gccaccgtcg cggcgaagaa gagcgcgtgg
     2101 agctgccacg gcgcgctgtg tttgtgcctg tagagcacgg cgccctcgac ggccatgtag
     2161 cgccgctcgt agaggtcggg cggccgcgtc ggatcga
//
LOCUS       DASWNJ010001097         2044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_90198, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001097 DASWNJ010000000
VERSION     DASWNJ010001097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90198"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1591)
                     /locus_tag="VGB85_18430"
     CDS             complement(<1..1591)
                     /locus_tag="VGB85_18430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136073.1"
                     /translation="MLAIAGLCVTAVGPAGCKPRGEATDAPFTTDTARARAAELERHL
                     KKEPTDDAAALELAHLYWLHLRAPAKATPILDRLAAAGDPVAQVSRMLIADPRLDMKT
                     TRTMAQALVRGAGKPATGPGASPERAAMQVALAELAARYLSENHGEQPDDDLDFSRFF
                     AEVERLELPATVSQPLQSLRASIARRLDQPYMGYYEAQGCVRAWQVGPVEGTLEAFEL
                     RRAAKDPGAFEADPQAVLTPLSCVVRTWNPTARAGMRRMRSFLSVPGDRLTLNLSSQE
                     PIRVYLDGVPVHRSDRSDRWPTGEATLAMDVKPGVQRLDVHTTVPRDKVWVLVRATDH
                     RGKAVPASMQATGAMATPFTGKPTRASSPFFPYDRGPLAGPSFAPLRMFLAASDAMAD
                     ANSDDAEGFIKALRQLGPNFAEGQLLVAAFESGDPTRERGASTARQRAAIERALALDP
                     ELARARLRLLELTLERGETAEVIDALAALGDQALRNVPGEMLRHAAFLARGNEHLADA
                     ALARAAAIHPEHCGVLKAQRNT"
BASE COUNT          256 a          711 c          780 g          297 t
ORIGIN      
        1 ccgtgttgcg ctgggccttg agcacgccgc agtgctcggg gtggatcgca gcggcgcgag
       61 cgagggccgc gtcggcgagg tgctcgttgc cgcgggcgag gaacgcggcg tggcgcagca
      121 tctcgccggg cacgttgcgc agggcctggt cgccgagcgc cgcgagggcg tcgatcacct
      181 ccgccgtctc gccgcgctcg agggtcagct cgagcaggcg caggcgcgcg cgcgcgagct
      241 cggggtcgag cgccagggcc cgctcgatcg ccgcgcgctg cctcgcggtc gaggccccgc
      301 gctcgcgcgt cgggtcgccg gactcgaagg cggcgacgag cagctggccc tcggcgaagt
      361 tcggcccgag ctggcgcagc gccttgataa aaccctcggc gtcgtcgctg ttggcgtcgg
      421 ccatcgcgtc cgaggcggcg aggaacatgc gcagcggcgc gaagctgggg ccggcgagcg
      481 ggccgcggtc gtaggggaag aacggggagg acgcgcgtgt cggcttgccg gtgaacggcg
      541 tcgccatcgc gccggtcgcc tgcatgctcg cgggcacggc cttgccgcgg tggtcggtcg
      601 cgcggacgag cacccacacc ttgtcgcgcg gcacggtggt gtgcacgtcg aggcgctgca
      661 cgccgggctt gacgtccatc gccagcgtcg cctcgccggt cggccagcgg tcgctgcggt
      721 cgctgcggtg gaccggcacg ccgtcgaggt agacgcggat cggctcttga ctactgaggt
      781 tgagggtcag gcggtcgccc ggcacgctca ggaagctccg catgcgtcgc atgccggcgc
      841 gcgcggtcgg gttccacgtg cgcacgacgc aggacagcgg cgtcagcacg gcctgcgggt
      901 cggcctcgaa ggcgcccgga tccttggccg cgcggcgcag ctcaaaggcc tcgagcgtgc
      961 cctcgaccgg gccgacctgc cacgcgcgca cgcagccctg cgcctcgtag tagcccatgt
     1021 acggctgatc gaggcggcgg gcgatgctcg cgcgcaggct ctgcagcggc tgcgagacgg
     1081 tcgccggcag ctcgaggcgt tctacttcgg cgaagaagcg actaaagtcg aggtcgtcgt
     1141 cgggctgctc gccgtggttc tccgacaggt agcgcgccgc gagctcggcc agcgcgacct
     1201 gcatcgccgc ccgctcgggc gacgcgccgg gccccgtcgc cggcttgccg gccccacgca
     1261 ccagcgcctg cgccatcgtc cgcgtggtct tcatgtcgag ccgcgggtcg gcgatcaaca
     1321 tgcgcgagac ctgggccacg gggtcacccg ccgccgcgag ccggtcgagg atcggcgtcg
     1381 ccttcgcggg cgcgcgcagg tgcagccagt acaggtgcgc cagctcgagc gcggcggcgt
     1441 cgtccgtggg ctccttcttc agatgccgct cgagctcggc cgcgcgagcg cgcgccgtgt
     1501 cggtcgtgaa cggcgcatcg gtggcctcgc cccgcggctt gcagcccgcg ggcccgacag
     1561 cggtcacgca gagccccgcg atcgcgagca tggtccgaag gacaggtccc aaggtcactg
     1621 tacgaagaga catgaatacc ggggtcccct caccggggct ttctcgactg tttgtggggc
     1681 tcgactgccc ggatgtcgcg tgctcgagcc acgcgggact cgactgcctg gatgtcgcgt
     1741 gctcgagata cacggcactc gactgcctgg atgtcgcgtg ctcgagatac acggcactcg
     1801 actgcctcga tgtcgcgtgc tcgagataca cggcactcga ctgcctcgat gtcgcgtgct
     1861 cgagatacgc ggcactcgac tgcctggatg tcgcgtgctc gagcgacgcg gcactcgact
     1921 gcctggatct cgcgtgcttg agcgacgcgg cactcgacgg cctggatgtc gcgtgctcga
     1981 gcgacgcggg gcgaggcgac ccgggccggg gggggccgac ggtctgcggg tccctgcgac
     2041 gcgg
//
LOCUS       DASWNJ010001098         2248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_20197, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001098 DASWNJ010000000
VERSION     DASWNJ010001098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20197"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VGB85_18435"
     CDS             complement(<1..562)
                     /locus_tag="VGB85_18435"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HEY0136074.1"
                     /translation="MRAIILAAGRGSRLAPLTDDRPKPLVEVLGRSLLLRTIDRLREV
                     GVEDRDVVIVTGYRAEMIEAVMRREDLRCELVANPRWHDWNSFNSLLVARDRLRGAPF
                     LQFEGDVVFDERVLPRVLGAPGGAVLAVEVVPMPDDEAMKAVVDADGVLLELSKTLDA
                     RRSIGEYVGITRLDVATGEQVFDDLAR"
     gene            complement(660..2186)
                     /locus_tag="VGB85_18440"
     CDS             complement(660..2186)
                     /locus_tag="VGB85_18440"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY0136075.1"
                     /translation="MKPTPYALAMVFDARTGARLDPLPDSLLAVVAGEALAGGRLGRL
                     VATTEYNRYYAVDRVPAVRASIVGTQPATLILSRDEGRTLRRLNGDSERFEWWYRTFH
                     VNQFTGHLALWTTLLYACAAGVVALIVFGYQLFWWRRPRGASAQRAAHNRAPRRWSRN
                     LHRKLGAVVGGVLAIQLLVGTYLWLSLGPLEDPFRGKASFSTDWRGGFPTRAALAEPG
                     TVLAQVAADPLPLRPDLRLHVQWVAPGSEPPEGVRVVRGDEVEGVRVVDEASRRQVER
                     ALCRTLAKSHQGLIDGWINWRFSMPITRVLSHTPVKPNHITLFSACVGLAAAASVLGG
                     TWASIALGGVLIQTQSILDSCDGELARLRFEGSKFGQWLDNITDDLLDLAFIVCAGVA
                     AGGVFLPLALAGSALRAFGHLAMYHEVVRRTGGGDVYSFRIWFERDSQQVDEVFGVHG
                     IGGYLRALGRRDTYVFVWMLLLLCGQVEVVAVYGAVLGTMIGVLMLLHIVLRPPLPRR
                     "
BASE COUNT          361 a          822 c          790 g          275 t
ORIGIN      
        1 agcgggcgag gtcgtcgaac acctgctcgc cggtggcgac gtcgaggcgc gtgatcccga
       61 cgtactcgcc gatcgatcgc cgcgcgtcga gggtcttcga cagctcgagc agcacgccgt
      121 cggcgtcgac gaccgccttc atcgcctcgt cgtccggcat cggcacgacc tcgaccgcca
      181 gcacggcccc gcccggcgcg ccgagcaccc gcggcagcac gcgctcgtcg aacacgacgt
      241 cgccctcgaa ttgcaggaac ggcgcgccgc gcaggcggtc gcgggcgacc aggagcgagt
      301 tgaagctgtt ccagtcgtgc cagcgcgggt tggcgaccag ctcgcagcgc aggtcctcgc
      361 ggcgcatgac cgcctcgatc atctcggccc ggtagccggt gacgatcacg acgtcgcggt
      421 cctcgacgcc gacctcgcgc agccggtcga tcgtgcgcag cagcaacgaa cggccgagga
      481 cctcgaccag gggcttgggg cggtcgtcgg tgagcggggc gaggcggctt ccacgaccgg
      541 cggcgagaat gatggcgcgc acggcgggca ctttatggca gcccgcgagg gagtcaacgc
      601 cgccgcacac cggcgggacc ccgacatcgt gacctcgcac acgaccgccc tggacgccct
      661 cagcggcgcg gcagcggcgg gcgcaggacg atgtgcagca gcatcagcac cccgatcatc
      721 gtcccgagca ccgcgccgta gacggcgacg acctcgacct gcccgcacag cagcagcagc
      781 atccacacga acacgtaggt gtcgcgccgg ccgagcgcgc gcaggtagcc gccgatgcca
      841 tggaccccga acacctcgtc gacctgctgg ctgtcgcgct cgaaccagat gcgaaagctg
      901 tacacgtcgc cgccgccggt ccggcgcacg acctcgtggt acatcgccag gtgtccgaac
      961 gcgcgcaggg ccgagccggc gagcgccagc ggcaagaaca cgccgcccgc ggccacgccg
     1021 gcgcagacga tgaacgcgag gtcgaggaga tcatcggtga tgttgtcgag ccactgcccg
     1081 aacttgctgc cctcgaagcg caggcgggcc agctcgccgt cgcaggagtc gaggatcgac
     1141 tgcgtctgga tcagcacgcc gccgagggcg atcgacgccc acgtcccgcc gagcacgctg
     1201 gccgccgccg cgaggccgac gcaggccgag aacagggtga tgtggttcgg cttgaccggc
     1261 gtgtgcgaca gcacccgcgt gatcggcatc gagaagcgcc agttgatcca gccgtcgatc
     1321 aggccctgat gcgacttggc gagcgtccgg cacagcgcgc gctcgacctg gcggcgcgag
     1381 gcctcgtcga cgacgcgcac gccctcgacc tcgtcgccac gaaccacccg cacgccctcc
     1441 ggcggctcgc tgcccggcgc gacccactgg acgtgcaacc gcaggtccgg gcgcagcggc
     1501 agggggtcgg ccgccacctg ggcgagcacg gtgccgggct ccgcgagcgc cgctcgggtg
     1561 gggaagcccc cgcgccagtc ggtgctgaag ctggccttgc cgcggaacgg gtcctcgagt
     1621 ggcccgaggc tgagccagag gtaggttccc acgagcagct ggatggccag cacgccgccg
     1681 accaccgccc cgagcttgcg gtgcaggttc ctcgaccagc ggcgcggcgc gcggttgtgt
     1741 gcggcccgct gcgcggacgc gccgcgtggc cggcgccacc agaacagctg gtagccgaac
     1801 acgatgagcg cgacgacgcc ggcggcgcac gcatagagca gcgtcgtcca tagggcgagg
     1861 tggcccgtga actggttcac gtggaaggtg cggtaccacc actcgaaccg ttcagagtcg
     1921 ccgttcagcc gccgcagcgt ccgaccctcg tcgcgcgaga ggatcagcgt cgccggctgc
     1981 gtgccgacga tgctggcacg caccgccggc acccgatcga cggcgtagta ccggttgtac
     2041 tccgtcgtgg ccacgagcct tccaagtcgc ccgccggcca gcgcctcgcc tgccacgacc
     2101 gccagcaggc tgtccggcag cgggtcgagt cgcgctccgg tgcgcgcatc gaagaccatg
     2161 gccagcgcat agggcgtcgg cttcacgacg taccacaggt atggtccgcg tgattggagc
     2221 cggagccagt agacacgctc gatcccgt
//
LOCUS       DASWNJ010001099         2770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_656391, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001099 DASWNJ010000000
VERSION     DASWNJ010001099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656391"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1845
                     /locus_tag="VGB85_18445"
     CDS             <1..1845
                     /locus_tag="VGB85_18445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136076.1"
                     /translation="LDGQISTLRHMTMGFAGVLLRDRVSDVRYDPRRDALSAEAPRPQ
                     AVLPKFFVQWDTDQWAILAGTYRAGFGQRLTFDNTRNYTPNGFYRDLTIYPRAVRLNS
                     LCNENAGELDQSPCQGDSGNTYATPDFRWQETLQGLAVGLKKLEMGPGWLQAYAFMSV
                     KGKTAYQYQLYDPEICEDPRNDEDPRCSAPPIYKRLPNPLAPTSEFAYQKLPQMYRDT
                     TAGGNVSYFFNRRSWVGVTGYGTDITWQTGGKPLGFQEWARTPFGGPFGAVGINASWG
                     KRWSDLGVEVSRSFDSMKAASGSDKTDARGGGGYAAIVRHTAAWDRHEVETIVRYYDR
                     NFANPYARPLSGNDVYEGQRARDEAGFRVRYTGRPAKKTSVRAYINYWVAPSTRISSI
                     HLLARVEHQVSRWFIPGVWFEGRDKNLAVNGPGNCYGGDPGVSSNIDSGDPDSFDPAE
                     TEVDYGNLMGDPAARCPGEALKYNLQAAFFPHRRVKLVPRFQHRFVGDPRSTAISEPM
                     MGYPKPAKVPREGYRQDLSAWLTITTRPIDSVRLRGRLRYLNQDIADNTYLEQSLWSY
                     VDVGYLLRKKFLIQVRYDLYVWLDKRASTLTRIPSPEHRLLLTLEGRF"
     gene            1855..2343
                     /locus_tag="VGB85_18450"
     CDS             1855..2343
                     /locus_tag="VGB85_18450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136077.1"
                     /translation="MSYDQIAARLRCPRCGLETRDEWAVFQMRWSFPAECTTYRPGDR
                     VHIKHPKYWSYHNMRGEIDFDAIDRLRILDTWDCDGCGPWNLAMTTLIIEDDCERIAS
                     VEAVPLSAETLRAADCVTDQIMYIYREVTDTRMLDDEGNSIEGWFESLIQALAARDPA
                     TG"
     gene            complement(2346..>2770)
                     /locus_tag="VGB85_18455"
     CDS             complement(2346..>2770)
                     /locus_tag="VGB85_18455"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEY0136078.1"
                     /translation="IWLDAGELEALVADKEPTGAEPPTMGELRARMREVVPSEAAVKY
                     RDCPRCQQVMRRTNFGTISGVVVDECVQHGVLLDPGELQAIETFVRMGGQALGDEVRI
                     QHGQRVRASIPPPPQIQTYEDRRRETAATTVFDLLFRW"
BASE COUNT          459 a         1055 c          854 g          402 t
ORIGIN      
        1 ctcgacggcc agatctcgac gctgcgccac atgaccatgg gcttcgccgg cgtgctgctg
       61 cgcgaccgcg tcagcgacgt ccgctacgac ccgcggcgcg acgcgctgag cgccgaggcc
      121 ccgcgcccgc aggccgtcct gcccaagttc ttcgtccagt gggacacgga ccagtgggcg
      181 atcctcgccg gcacctaccg cgccggcttc ggccagcgcc tcaccttcga caacacccgc
      241 aactacaccc ccaacggctt ctaccgcgac ctgacgatct acccccgcgc cgtccggctc
      301 aacagcctgt gcaacgagaa cgccggcgag ctcgaccaga gcccctgcca gggcgacagc
      361 ggcaacacct acgcgacccc cgactttcgc tggcaagaga ccctccaggg cctcgccgtc
      421 ggcctcaaga agctcgagat gggcccgggc tggttacagg cctacgcgtt catgtccgtc
      481 aagggcaaga ccgcctatca gtaccagctc tacgaccccg agatctgcga ggacccgcgc
      541 aacgacgagg acccgcgctg ctcggcccca ccgatctaca agcggctgcc caacccgctc
      601 gccccgacct ccgagttcgc gtatcaaaag ctcccgcaga tgtaccgcga caccaccgcg
      661 ggcgggaacg tcagctactt cttcaatcgc cgcagctggg tcggcgtcac cggctacggc
      721 accgacatca cctggcagac cggcggcaag ccgctcggct ttcaggagtg ggcgcgcacc
      781 ccgttcggcg ggccgttcgg cgccgtcggc atcaacgcct cgtggggcaa gcgctggagc
      841 gacctcggcg tcgaggtgtc acgcagcttc gactcgatga aggccgccag cggcagcgac
      901 aagaccgacg cgcgcggcgg aggcggctac gcggcgatcg tgcgtcacac ggccgcctgg
      961 gaccgccacg aggtcgagac gatcgtccgc tactacgacc gcaacttcgc caacccctac
     1021 gcgcgcccgc tgtcgggcaa cgacgtctac gagggccagc gcgcccgcga cgaggccggc
     1081 tttcgcgtcc gctacaccgg ccgcccggcc aagaagacca gcgtccgcgc ctacatcaac
     1141 tactgggtcg cgccgtcgac ccgcatctcg tcgatccacc tgctcgcccg cgtcgagcat
     1201 caggtcagcc gctggttcat ccccggcgta tggttcgagg gccgcgacaa gaacctcgcc
     1261 gtcaacggcc ccggcaactg ctacggcggc gaccctggcg tctcgagcaa catcgacagc
     1321 ggcgaccccg acagcttcga ccccgccgag accgaggtcg actacggcaa cctgatgggc
     1381 gacccggccg cgcgctgccc cggcgaggcg ctcaagtaca acctccaggc ggccttcttc
     1441 ccgcaccgcc gcgtcaagct ggtcccccgc ttccagcacc gcttcgtcgg cgacccgcgc
     1501 tcgaccgcga tctccgagcc aatgatgggt taccccaagc ccgccaaggt cccgcgcgag
     1561 ggctatcgcc aggacctctc cgcctggctg acgatcacca cccgaccgat cgactccgtg
     1621 cgcctgcgcg gccgtctgcg ctacctcaac caggacatcg ccgacaacac ctacctcgag
     1681 cagagcctgt ggtcgtacgt ggacgtcggc tacttgctgc gcaagaagtt cctgatccag
     1741 gtccgctacg acctttatgt ttggctcgat aagcgcgcaa gtaccctcac gcgcatcccc
     1801 tcgcccgagc accgcctgct gctgaccctc gagggtcgct tctaaggcgc gccgatgtcc
     1861 tacgatcaga tcgccgcccg cctgcgatgc ccgcgctgcg gcctcgagac ccgcgacgag
     1921 tgggcggtgt ttcagatgcg ctggtccttc cccgcggagt gtacgacgta tcgcccaggg
     1981 gaccgggtgc acatcaagca cccgaagtac tggagctacc acaacatgcg cggcgagatc
     2041 gactttgacg cgatcgatcg cctgcggatc ctcgacacgt gggactgcga cggctgcggc
     2101 ccgtggaacc tggcgatgac gaccctgatc atcgaggacg actgcgagcg gatcgcgtcc
     2161 gtcgaggcgg tcccgttgag cgcggagacc ctccgcgccg ccgactgcgt gaccgaccag
     2221 atcatgtaca tctatcgcga ggtcacggac acgcggatgc tcgacgacga gggcaacagc
     2281 atcgagggct ggttcgagtc gctgatccag gcgctcgcgg ctcgcgaccc cgcgaccggc
     2341 tgacctcacc agcgaaacag caggtcgaat acggtcgtcg ccgcagtctc tcgccgccgg
     2401 tcctcgtagg tctggatctg cggcggcggt gggatcgaag cgcgcacgcg ttgcccgtgc
     2461 tggatccgga cctcgtcgcc gagcgcctgc ccgcccatgc gcacgaaggt ctcgatcgcc
     2521 tgcagctcgc cggggtcgag cagcacgccg tgctgcacgc actcgtcgac gaccacgccg
     2581 ctgatcgtcc cgaagtttgt gcgccgcatg acctgctggc agcgcgggca gtcgcggtac
     2641 ttgaccgcgg cctcggacgg gaccacctcg cgcatgcggg cccgcagctc gcccatcgtc
     2701 ggcggctcgg cccccgtggg ctccttgtcg gcgacgagcg cctcgagctc gccggcgtcg
     2761 agccagatcg
//
LOCUS       DASWNJ010001100         2139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_20250, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001100 DASWNJ010000000
VERSION     DASWNJ010001100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20250"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="VGB85_18460"
     CDS             complement(<1..983)
                     /locus_tag="VGB85_18460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEY0136079.1"
                     /translation="MVARNDRTAGARPITLAVTPGQPRLAWHAAEVTHGSPRAATLVE
                     RVGAGAAVGRIVHAEDNLEALHALAAEGLAGKVDLVYIDPPFMAGGDREATQGSAMAY
                     RDTWPGGLDGYLDMLRARLLAIRPLLAATGNLFVHLDWHAVHYVKVLCDELLGAANFR
                     NEIVWRRANAHSDSQRFGVITDAILFYGASARAHWADVHVPHEPRYIASHWRRSDARG
                     RYRLVPLDAPRHGEGGNLVYPWRGKTPAPTRTWAIGREAMEALERAGLIAYTASGTPT
                     RKRYLADSPGLRAQNLWDDIPPVNPMAGERLGYPTQKPVALLDRIIKASSNP"
     gene            complement(1007..>2139)
                     /locus_tag="VGB85_18465"
     CDS             complement(1007..>2139)
                     /locus_tag="VGB85_18465"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0136080.1"
                     /translation="EYQELTRGQPPGSPPPSLAELWALRFRRSETYSRFVHDRLEKAP
                     SRVGEGQASGDDANASRTSIAMKVAANRDVRPAIAPVSGLRQFGILVRRYLRLMLSDR
                     RTLAILLLQAPIIGGILLLVAKEDALQNLESSHGRLVLFLLALVAVWFGILNSAREIS
                     KEQAIYRRERLANLRIGPYVASKVCVLAGLCLVQSLVLLLVLAIKVDFSAEVVQFTGT
                     GPVHHMRGEFLLPLGFVGALAITTFLTSMTGLGLGLLISSAANTSDRAMSVIPIALVP
                     QIVFALALMPLPATLAWVSYATSSRFAMEALGAISRLPLPRDYSDCAIPGNPRSCAIY
                     PSVDYDPAVSHVLTLWGSLAGYALVCLLATTMILVARDRQRS"
BASE COUNT          318 a          760 c          773 g          288 t
ORIGIN      
        1 gggttgctgc tggccttgat gatgcgatct agcagggcga ccggcttctg cgtcgggtag
       61 ccgaggcgct cgccggccat cgggttgacc ggcgggatgt cgtcccacag gttctgcgcg
      121 cgcaggcccg gcgagtcggc gaggtagcgc ttgcgcgtcg gcgtgccgct ggccgtgtac
      181 gcgatcaggc ccgcgcgctc gagcgcctcc atcgcctcgc ggccgatcgc ccacgtgcgc
      241 gtcggggccg gcgtcttgcc gcgccacggg tacaccaggt tgccgccctc accgtggcgc
      301 ggcgcgtcga gcgggaccag gcggtagcgg ccgcgggcgt cgctgcggcg ccagtggctg
      361 gcgatgtagc gcggctcgtg tggcacgtgc acgtcggccc agtgcgcccg cgccgacgcc
      421 ccgtaaaaca ggatcgcatc ggtgatcacg ccgaagcgct gcgagtcact gtgcgcgttg
      481 gcgcgacgcc acacgatctc gttgcgaaag ttcgcggcgc cgagcagctc gtcgcacagc
      541 accttgacgt agtgcaccgc gtgccagtcg aggtgcacga acaggttgcc ggtcgccgcg
      601 agcaagggac ggatcgccag caggcgcgca cgcagcatgt cgaggtaccc gtcgaggccg
      661 cccggccagg tgtcgcggta ggccatcgcg ctgccctgcg tcgcctcgcg atcgccgccg
      721 gccatgaacg gcgggtcgat gtagaccagg tcgaccttgc ccgccagacc ctcggccgcg
      781 agcgcgtgca gggcctcgag gttgtcctcg gcgtgcacga tcctgccgac cgccgcgccg
      841 gccccgacgc gctcgacgag cgtcgcggcg cgcggtgatc cgtgcgtgac ctcggccgcg
      901 tgccaggcca ggcgcggctg accgggcgtc accgccagcg tgatcgggcg ggcgccagcc
      961 gtgcggtcgt tacgcgccac catcgctcac gaccgctcac gaccgctcac gaccgctggc
     1021 gatcgcgggc gacgaggatc atcgtcgtcg ccaggaggca gaccagcgcg tagccggcga
     1081 gcgaccccca cagcgtcagg acgtgggaaa ctgccgggtc gtagtcgacc gaggggtaga
     1141 tcgcgcagct gcgcgggttc cccgggatcg cgcagtccga gtagtcgcgc ggcagcggca
     1201 gccggctgat cgcgccgagg gcctccatcg cgaaccggct gctcgtcgcg tacgagaccc
     1261 acgcgagggt cgccggcagc ggcatcaggg ccagcgcgaa gacgatctgg ggcaccagcg
     1321 cgatcgggat gacgctcatc gcccggtccg aggtgttggc cgcgctcgag atcagcaggc
     1381 cgagcccgag gcccgtcatc gacgtcagga acgtcgtgat cgccagcgcg ccgacgaagc
     1441 cgagcggcag caggaactcg ccgcgcatgt ggtgcacggg gccggtgccg gtgaactgca
     1501 cgacctccgc cgagaagtcg accttgatcg ccagcaccag cagcagcacc aggctctgga
     1561 ccaggcacag cccggcgagc acgcacacct tcgatgcgac gtagggcccg atccgcaggt
     1621 tggcgaggcg ctcgcggcga tagatcgcct gctccttgct gatctcgcgc gcgctgttca
     1681 agatcccgaa ccacaccgcg acgagcgcga gcaggaacag caccaggcgt ccgtgcgagg
     1741 actcgaggtt ctgcagcgcg tcctccttgg cgacgagcag caagatcccg ccgatgatcg
     1801 gcgcttgcag cagcaagatc gccagcgtgc ggcgatccga gagcatcagc cgcaggtatc
     1861 gccggaccag gatcccgaac tggcgcaggc ccgagaccgg cgcgatcgcg ggtcgcacgt
     1921 cgcggttggc cgcgaccttc atggcgatcg aagtccgcga cgcgttcgcg tcgtccccgg
     1981 acgcctggcc ttcgccgacc cggctcggcg ccttctcgag gcggtcgtgt acgaagcgac
     2041 tgtaggtctc cgaccggcga aagcgcagcg cccacagctc ggccaggctc ggcggcggcg
     2101 agcccggcgg ctgcccgcgc gtcagctcct ggtactcgc
//
LOCUS       DASWNJ010001101         3361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_402566, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001101 DASWNJ010000000
VERSION     DASWNJ010001101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402566"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VGB85_18470"
     CDS             <1..344
                     /locus_tag="VGB85_18470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136081.1"
                     /translation="QTRSGAPRDAAIAVDYVIIADASGRATIENLAIADVVAPEDKLG
                     PHRASCSLFLGDEVEVVARFKPERFGEDPDPSRLVYGTLGADGNLHVRVCKRASGPTA
                     DPSQPSGTTTS"
     gene            341..1189
                     /locus_tag="VGB85_18475"
     CDS             341..1189
                     /locus_tag="VGB85_18475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136082.1"
                     /translation="MKRTLLCLTAFALLGACKKDPATTPPDDTKSGDTAGTPTGGATG
                     GTQTGIPQEPDPPAIAQAREQYMLGNYDKAKELLEPLTADLKTRQQLRASGLSAGWLA
                     LAVSDPVAENAKEPAEHAVAMGDQTGDKEVQIVAKLAIGSYQLGMDDPATAATSFEAA
                     YKLQTDGPNASLALVMYGQAKVNMAFGGEDKDQIAHPQELDSAASTFVKAQRLAASQP
                     GNELVAARAYEGIASVANYKKNFKEACTQIAEAVKIYQAKSAGQPLIERVNAIMDAAN
                     CQKAAG"
     gene            1287..2915
                     /gene="lysS"
                     /locus_tag="VGB85_18480"
     CDS             1287..2915
                     /gene="lysS"
                     /locus_tag="VGB85_18480"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY0136083.1"
                     /translation="MESHDPYLNHWADQAAAKTLTAHPEAKVITVAAGITPSGVVHVG
                     NFREVMTVDLVARALRDRGAQVRFIYSWDDFDVFRKVPKGMPKAELLAANLGRSIVDV
                     PDPYDQHDSYASHHIAQFEDSLKPLGIAPEFIRQSKRYRAGAYAEGIRKALAGAGKIR
                     EILNSVREQTTASTRIEDDWLPLSGFCDECGRDKLRFRVTGEWTVHYTCDKCKHEREV
                     DLREGGNLKLPWRIDWPMRWAHEGVCFEPGGKDHSTAGGSYDTAKDIVREVYDWRAPE
                     YVGYDFVRIKGRAGKISSSSGEVVTVADCLEIYEPEVLRWIFVSYRPNTEFQISFDLD
                     VIALYESYDRLCRLAYGLEEAKDEQKKLAAQRTFQLASVDHHRIAAGEAMPFVPGFRH
                     LSMILQIHDGDLDRTVAHYEQSGQIKTDAERLACRRRAACVRNWLGSYATDEFRYTIR
                     REPRTRALAPDEVTCLTRLVEVIRQNPDIDEDGLVPTMKTLTAGTSLDNKTFLPVVYD
                     LLIDRDKGPKLTTLVTAMGTRRALSLLEPSLAAS"
     gene            complement(2922..>3361)
                     /locus_tag="VGB85_18485"
     CDS             complement(2922..>3361)
                     /locus_tag="VGB85_18485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136084.1"
                     /translation="APAKEPAKMAQAVLAFNEQFERIAEVALVLCIGAMVPQVSWPEH
                     TAWFLVVLLLGIRPLAVWLGLLGAGLSGVQVAFMSWFGVRGVGSLNYLTVALVVGASG
                     GEVQTLTDLTLIVIAASILLHGVSVTPLMRRYQKTPECSDAPA"
BASE COUNT          589 a         1228 c         1105 g          439 t
ORIGIN      
        1 ggcaaacccg cagcggggcc ccgcgcgacg ccgcgatcgc ggtcgactac gtcatcatcg
       61 ccgacgccag cgggcgcgcg acgatcgaga acctcgcgat cgccgacgtc gtcgcgccgg
      121 aggacaagct cgggccgcac cgggcttctt gctccctctt cctgggcgac gaggtcgagg
      181 tcgtggcgcg gttcaaaccc gagcgcttcg gagaagatcc cgatccctcg cgcctggttt
      241 acggcacact cggcgcagac gggaaccttc acgtccgcgt gtgcaagcgc gcctccggcc
      301 ccactgccga tccatcgcaa ccgtcaggaa ccacgacctc atgaagcgca ccctgctctg
      361 cctcaccgcc ttcgccctgc tcggcgcctg caagaaggac cccgccacca ctccgcccga
      421 cgacacgaag tccggcgaca ccgcgggcac cccgaccggc ggcgccacgg gtggcacgca
      481 gaccggcatc ccgcaggagc ccgatccgcc ggcgatcgcc caggcgcgtg agcagtacat
      541 gctcggcaac tacgacaagg ccaaggagct gctcgagccg ctgaccgccg acctcaagac
      601 gcgccagcag ctgcgcgcct cgggcctgag cgccggctgg ctcgcgctcg cggtctccga
      661 cccggtcgcc gagaacgcca aggagccggc cgagcacgcc gtcgccatgg gcgaccagac
      721 cggcgacaag gaggtccaga tcgtcgccaa gctggcgatc ggcagctacc agctcggcat
      781 ggacgacccg gcgaccgcgg cgaccagctt cgaggcggcg tacaagctgc agaccgacgg
      841 cccgaacgcc agcctcgcgc tcgtcatgta cggccaggcc aaggtcaaca tggccttcgg
      901 cggcgaggac aaggaccaga tcgcccaccc gcaggagctc gactctgccg cctcgacctt
      961 cgtcaaggcg cagcgcctcg cggcctcgca gccgggcaac gagctggtcg ccgcgcgcgc
     1021 gtacgagggc atcgcctcgg tcgccaatta taagaagaac ttcaaggagg cctgcacgca
     1081 gatcgcggag gccgtcaaga tctaccaggc caagagcgcc ggccagccgc tgatcgagcg
     1141 ggtcaacgcg atcatggacg ccgccaattg tcagaaggcc gctggctgag gccgggtctt
     1201 cgctcactcc cgccggtgat ggccgcactc ttcaggtccg ggggccttgt gcccacgggc
     1261 ctgtttgcgc gacaaccgtg cacgccatgg agtcgcacga cccctacctg aaccactggg
     1321 ccgaccaggc cgctgccaag accctcaccg cgcaccccga ggcgaaggtc atcaccgtcg
     1381 ccgccggcat cacgccctcg ggcgtcgtgc acgtcggcaa cttccgcgag gtgatgacgg
     1441 tcgacctcgt cgcccgcgcg ctgcgtgacc gcggcgcgca ggtccgcttc atctactcgt
     1501 gggacgactt cgacgtgttc cgcaaggtcc cgaagggcat gccgaaggcg gagctgctgg
     1561 cggcgaacct cgggcgcagc atcgtcgacg tgccggaccc ctacgaccag cacgacagct
     1621 acgcctcgca ccacatcgcg cagttcgagg acagcctcaa gccgctgggc atcgccccgg
     1681 agttcatccg ccagtcgaag cgctaccggg ccggggcgta cgccgagggc atccgcaagg
     1741 cgctcgccgg cgcggggaag atccgcgaga tcctcaacag cgtgcgcgag cagacgaccg
     1801 cgagcacccg catcgaggac gactggctgc cgctgtcggg cttttgcgac gagtgcgggc
     1861 gcgacaagct gcgctttcgc gtcaccggcg agtggacggt ccactacacc tgtgacaagt
     1921 gcaagcacga gcgcgaggtc gacctgcgcg agggcggcaa cctcaagctg ccgtggcgca
     1981 tcgactggcc gatgcgctgg gcgcacgagg gcgtgtgctt cgagcccggc ggcaaggacc
     2041 acagcaccgc gggcggcagc tacgacaccg ccaaggacat cgtgcgcgag gtctacgact
     2101 ggcgcgcgcc ggagtacgtc ggctacgact tcgtgcggat caagggccgc gccggcaaga
     2161 tctcgagctc gagcggcgag gtcgtcaccg tcgcggactg cctcgagatc tacgagccgg
     2221 aggtcctgcg ctggatcttc gtgtcgtacc gcccgaacac cgagttccag atcagcttcg
     2281 acctcgacgt catcgccctc tacgagtcct acgatcgcct gtgccggctc gcgtacgggc
     2341 tcgaggaggc caaggacgag cagaagaagc tcgccgcgca gcgcaccttc cagctcgcca
     2401 gcgtcgatca tcaccggatc gccgccggcg aggcgatgcc cttcgtgccc ggctttcgcc
     2461 acctgagcat gatcttgcag atccacgacg gtgacctcga tcgcaccgtc gcgcactacg
     2521 aacagagcgg gcagatcaag accgacgccg agcgcctcgc gtgccggcgg cgcgccgcgt
     2581 gcgtgcgcaa ctggctgggg agctacgcga ccgacgagtt ccgctacacg atccgccgcg
     2641 agcccaggac ccgcgcgctc gcgccggacg aggtcacctg cctgacgcgc ctcgtcgagg
     2701 tgatccggca aaacccggac atcgacgagg acggcctcgt gccgaccatg aagacgttga
     2761 ccgccgggac ctcgctcgac aacaagacct tcctgccggt cgtctacgac ctgctgatcg
     2821 accgcgacaa ggggcccaag ctgacgacgc tggtcacggc gatgggcacg cgccgcgcgc
     2881 tgtcgttgct cgagccgagc ctcgcggcgt cgtagtctcg atcatgccgg cgcgtcgctg
     2941 cactccgggg tcttttgata gcgccgcatc agcggcgtca ccgagacccc gtgcagcagg
     3001 atcgacgcgg cgatgacgat cagcgtcagg tcggtgaggg tctgcacctc accgcccgag
     3061 gcgccgacga cgagcgcgac ggtgaggtag ttgagcgagc cgacgccgcg cacgccgaac
     3121 cagctcatga acgcgacctg cacgccggac agcccggcgc cgagcagccc gagccacacc
     3181 gcgagcgggc ggatccccag cagcaggacg accaggaacc acgcggtgtg ctcgggccat
     3241 gacacctgcg ggaccatcgc gccgatgcac agcaccagcg cgacctcggc gatgcgctcg
     3301 aattgctcgt tgaaggcgag cacggcctgg gccatcttcg ccggctcctt cgcgggcgcg
     3361 t
//
LOCUS       DASWNJ010001102         5302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_125100, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001102 DASWNJ010000000
VERSION     DASWNJ010001102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125100"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..630)
                     /locus_tag="VGB85_18490"
     CDS             complement(85..630)
                     /locus_tag="VGB85_18490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0136085.1"
                     /translation="MIVDLDPGQPDLLEQAAALLVARMPEGWPTLADARDHVSELLDP
                     GFILRGYIRGRELLGWVGGQERYTGHVWELHPLVVHASHERRGIGRALVLDLEDRVRE
                     LGIHSMFVGSDDELGLTSLAGVDLYPDVIGHLARLANLGDHPIAFYKRLGYSVIGVMP
                     DANGFGQPDIYLGKRLIARAG"
     gene            complement(648..1556)
                     /locus_tag="VGB85_18495"
     CDS             complement(648..1556)
                     /locus_tag="VGB85_18495"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0136086.1"
                     /translation="MAALVPVKADLSAKTCIVTGASSGVGKEIARNLAAMGATVIVTS
                     PGLDRADAARADIVASTQSLRVLAMKLDYTNRLSIREFAETFAERHERLDVLVHSAAA
                     WSPDREQTVDAIEMTWTVNVLGPHILNRLLTDKLKASAPARIIHLACAHAGALDVDDT
                     DFDRRRYDGWAAFRQSQQAIRMLATSLSPRMAIHGVNVHVCIPGANVRTNLHRKARGL
                     RGLRLKLAAKLFGVSPAAAADGPTWLAAEPNVGPGKLWVGRKESERRFRDAAEIQRLW
                     DSVERQANGLMSKPGLGQSGLFTLPT"
     gene            complement(1721..1960)
                     /locus_tag="VGB85_18500"
     CDS             complement(1721..1960)
                     /locus_tag="VGB85_18500"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136087.1"
                     /translation="MPKKIFIGSLSVATTDSTINTQFSPFGTIVAAAVNRDASGASLG
                     NANVEYTADQAGTDAITAKNGTQIDGSTISVVPAR"
     gene            complement(2015..2536)
                     /locus_tag="VGB85_18505"
     CDS             complement(2015..2536)
                     /locus_tag="VGB85_18505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136088.1"
                     /translation="MGMFRSIIDLFRRLLGLSREPVLSLGSGTQTNAKPAVPSLDEDD
                     DDAEDGDAGPDDATRDGQLSIEDEWSELQILIARREAEGIDLAGLDINDPASFWARQL
                     RIEEHMRQGKGRLHSVVAAGFRGLEHWDEVSRYFQAKWSQLARRDTGELEIRPRQEFT
                     DAAVRARPPVDRQ"
     gene            complement(2727..4427)
                     /locus_tag="VGB85_18510"
     CDS             complement(2727..4427)
                     /locus_tag="VGB85_18510"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY0136089.1"
                     /translation="MLDHDDNLPGETPARYGCLECQEDAELALADRLHPTAVARYRAH
                     LRACGDCRRMNQLLEAVYRGPDPASSGLGTLRESSSDREFAAILRRVPLERPLPWYQR
                     AAQTTGVVALATTAAALALALVLPAEDPLLEAEPLGGISAALQGARQGLRAAESSASE
                     DSAVAPHQAQVDYGRVIAGRGFLSTRGDGPPVLTDTFTVGTRFSVELGQSLQVGLLGR
                     ILANFEPGTEIEWTQAGPAALDLTLERGHIAVRYERQPTDPILNIHTPSALVRVVGTV
                     FTVEVDAEGSTNVAVLRGKVEVLNPEDQAHVADVQAGYRFELERMTYGDVGRHDVRVA
                     MPLSDEVPGSPAHAGELVSLASVTTSADGTIPASWVVPGLPAELPQRRLENILTRTPA
                     PEFFFERPSIVRPDRRYLSSLAAAKAEDGQSLIDKLVRDAAETRLEEGRAALELCKTL
                     YQSAQTRYLSARCLTQFMAEHGENDGLVEGHLLIGILRMDFANDYPAAKLAFERFLER
                     APAHPEAELARYRLWLASIEDGDVNGAIDRGREYLRRYPNGKYAGKILQRFPRLVSEL
                     "
     gene            complement(4449..5072)
                     /locus_tag="VGB85_18515"
     CDS             complement(4449..5072)
                     /locus_tag="VGB85_18515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY0136090.1"
                     /translation="MQLTQLTQAIDLAADVPDVAEDGARAGDYLTAEEQVMLARARAG
                     NPRALRQFYDTHQSQVRGHLYRLLGPDSEIDDLVQTIFARAFAALASFKANSTLSTWL
                     YRITTNTTHNLLRHRFRRNRVKSAFSWWLSGRAGALHNNHADVRDEALRLLQHLAPDL
                     REVFVFYHYEGLTLQEIAQILDKPLSTIGDRLTRARKRLHELVTTTP"
BASE COUNT          763 a         1697 c         2036 g          806 t
ORIGIN      
        1 gctcgacagc gtggtcgcgc ccggctttcg cccggccgca gcgagcaagc cggcgcgcaa
       61 caagccccgc ggacgcagca agcgctagcc cgctcgcgcg atcagccgct ttcctaaata
      121 aatgtcgggc tggccgaagc cgttggcgtc gggcatgacg ccgatgacgc tgtagccgag
      181 gcgtttataa aaggcgatcg ggtggtcgcc gaggttggcc aggcgcgcga ggtggccgat
      241 gacgtcggga tataggtcga cgccggcgag gctggtcagg ccgagctcgt cgtccgagcc
      301 gacgaacatg ctgtggatcc cgagctcgcg tacgcggtcc tcgaggtcga gcacgagcgc
      361 gcggccgatg ccgcggcgct cgtgactggc atgtacgacg agcgggtgca gctcccagac
      421 gtggccggtg tagcgctcct ggccgccgac ccagccgagc agctcgcggc cgcggatgta
      481 gccgcgcagg atgaagccgg ggtcgagcag ctcgctgacg tggtcgcgcg cgtcggcgag
      541 cgtcggccag ccctcgggca tgcgggcgac cagcagcgcc gcggcttgct cgagcaggtc
      601 aggctggccg ggatcgaggt cgacgatcac ctcgagatct tagcgggtca ggtcggcagc
      661 gtgaacaggc cggactggcc gagcccgggc ttcgacatca ggccgttggc ctggcgctcg
      721 acgctgtccc acaggcgctg gatctcggcg gcgtcgcgga agcggcgctc gctctccttg
      781 cggccgaccc acagcttgcc ggggccgacg ttgggctcgg cggcgagcca ggtcgggccg
      841 tcggcggcgg cggccgggct gacgccgaac agcttcgcgg cgagcttgag acgcaggcca
      901 cgcaggccgc gggccttgcg gtgcaggttg gtgcggacgt tggcgccggg gatgcacacg
      961 tgcacgttga cgccgtggat cgccatgcgc ggcgagagcg aggtcgccag catgcggatc
     1021 gcctgctggc tctggcgaaa cgcggcccag ccgtcgtagc ggcggcggtc gaagtcggtg
     1081 tcgtcgacgt cgagcgcgcc ggcgtgggcg caggcgaggt ggatgatccg cgctggcgcg
     1141 ctggccttga gcttgtcggt cagcaggcgg ttgaggatgt gcggcccgag cacgttgacg
     1201 gtccaggtca tctcgatcgc gtcgaccgtc tgctcgcggt cgggcgacca ggcggcggcg
     1261 ctgtggacca gcacgtcgag gcgctcgtga cgttcggcga aggtctcggc gaactcgcgg
     1321 atcgagaggc gattggtgta gtcgagcttc atcgccagga cccgcaggct ctgggtcgag
     1381 gcgacgatgt cggctcgcgc cgcgtcggcg cggtcgaggc cgggcgaggt gacgatgacg
     1441 gtcgccccca tcgccgcgag gttgcgggcg atctccttgc cgacaccgct gctcgcgccc
     1501 gtaacgatac aagtcttggc cgagagatcg gcttttaccg ggaccagtgc tgccatacgc
     1561 gaactccgaa cgagggggac cggagtatat caatcaggaa ccggcgaagt tgctccacga
     1621 aggatcatgc gatcgcaagc acaggtgcgc gttgcgtcta catgctccgc gagcggccgg
     1681 cggcgcgtga aagcgcgccg ccgggccggg tcgaccggcg tcagcgcgcg gggacgacgc
     1741 tgatcgtgct gccgtcgatc tgggtgccgt tcttggcggt gatggcgtcg gtgccggcct
     1801 ggtcggcggt gtactcgacg ttggcgttgc cgagcgaggc gccgctcgcg tcgcggttga
     1861 cggccgcggc gacgatcgta ccgaacggtg agaactgtgt gttgatggtc gagtcggtgg
     1921 tggcgacgct gaggctaccg ataaagattt tcttgggcat gatccctcga ttgttgcggg
     1981 tgtctacggt tgcggtgcga agcgaggaaa aggatcactg ccggtcgaca ggagggcgcg
     2041 cgcggacggc ggcgtcggtg aattcctgac gcgggcggat ctccagctca cccgtgtcgc
     2101 gccgtgcgag ctgcgaccac ttggcctgga aatagcggct gacctcgtcc cagtgctcga
     2161 ggccgcgaaa gccggccgcg acgacgctgt gcagccggcc cttgccctgg cgcatgtgct
     2221 cctcgatgcg cagctggcgg gcccagaagc tcgcggggtc gttgatgtcg aggccggcga
     2281 ggtcgatgcc ctcggcctcg cgccgggcga tcaggatctg cagctcggac cactcgtcct
     2341 cgatgctgag ctgaccgtca cgcgtggcat cgtcggggcc ggcatcaccg tcctcggcgt
     2401 cgtcgtcgtc ctcgtcgagc gacggaacgg ccggcttcgc gttggtctgc gtacctgacc
     2461 cgagagacag caccggctcc cgggagaggc ccagcagccg acgaaatagg tcgatgatcg
     2521 agcggaacat tcccatgctg gcgtctccga cgggacgcgg tcacatcaag ctcgctggga
     2581 tactaccgcc tagaggaagt gtccagatat cccggacggg tgcaccgcgc aacttgatcg
     2641 ccagctacgg tcgcttgtac ggccgaccgg gcgaaaacga tccatcgcgg acgccgcgac
     2701 cgcgatgatg tcgccgggcg ggccggtcag agctcggaga ccagccgcgg gaagcgctgc
     2761 aggatcttgc cggcgtactt gccgttggga taccggcgca ggtactcgcg gccgcggtcg
     2821 atcgcgccgt tgacgtcgcc gtcctcgatg ctggccagcc acaggcgata acgggcgagc
     2881 tcggcctccg ggtgggccgg cgcgcgctcg aggaagcgct cgaacgccag cttggccgcc
     2941 ggatagtcgt tggcgaagtc catgcgcagg atgccgatca gcaggtggcc ctcgacgagg
     3001 ccgtcgttct cgccgtgctc cgccatgaac tgcgtcaggc agcgcgccga gaggtagcgg
     3061 gtctgcgccg actgatacag cgtcttgcac agctcgagcg cggcgcgccc ctcttccagg
     3121 cgcgtctcgg cggcgtcgcg cacgagcttg tcgatcagcg actggccgtc ctcggccttg
     3181 gcggcggcga gcgagctcag gtagcgccgg tcggggcgca cgatgctcgg ccgctcgaag
     3241 aagaactcgg gcgcgggcgt gcgcgtcaga atgttctcga ggcgccgctg cggcagctcc
     3301 gcgggcaagc ccggcacgac ccagctcgcc gggatcgtgc cgtcggcgct ggtggtgacg
     3361 ctggcgagcg acaccagctc gccggcgtgc gcgggcgagc ccgggacctc gtcggacagc
     3421 ggcatggcga cgcggacgtc gtggcgaccg acgtcgccgt aggtcatgcg ctcgagctcg
     3481 aagcggtagc cggcctggac atcggcgacg tgggcctggt cctcggggtt gagcacctcg
     3541 accttgccgc gcaacacggc gacgttggtg ctgccctcgg cgtcgacctc gacggtgaac
     3601 acggtgccga cgacgcggac cagcgcggag ggcgtgtgga tgttgaggat cgggtcggtc
     3661 ggctggcgct cgtagcggac ggcgatgtgg ccgcgctcga gcgtgaggtc gagggcggcc
     3721 gggccggcct gcgtccactc gatctcggtc cccggctcga agttggcgag gatccgcccg
     3781 agcaggccga cctgcagcga ctggccgagc tcgaccgaga agcgggtgcc gacggtgaag
     3841 gtgtccgtca ggacaggtgg cccgtcgcca cgagtcgaca ggaagccgcg accggcgatc
     3901 acgcggccat agtcgacctg ggcctgatgc ggggcgacgg cgctgtcctc ggaggcgctc
     3961 gactcggcgg cgcgcaggcc ctgccgcgcg ccctgcagcg cggccgagat cccgccgagt
     4021 ggttcggcct cgagcaaggg gtcctcggcg gggagcacga gggcgagggc gagggcggcg
     4081 gcggtggtcg cgagcgcgac gacgcccgtg gtctgcgcgg cgcgctggta ccacggcagc
     4141 ggccgctcga gcggcacgcg acgaaggatc gcagcgaact cgcgatcgct ggaggactcg
     4201 cgcagggtgc cgaggccgga cgaggccggg tcggggccgc gatagacggc ctccagcagc
     4261 tggttcatgc gccgacagtc gccgcaggcc cgcaggtgag cccggtagcg ggcgacggcg
     4321 gtcgggtgca ggcggtcggc gagcgcgagc tcggcgtcct cctggcactc gaggcagccg
     4381 tagcgcgcgg gggtctctcc cggcaggttg tcgtcgtggt cgagcatggg atggatggga
     4441 tggcgagctt acggcgtggt ggtcacgagc tcgtggaggc gcttgcgggc gcgggtgagg
     4501 cggtcgccga tcgtcgagag cggcttgtcg aggatctggg cgatctcctg cagcgtcagg
     4561 ccctcgtagt gatagaagac gaagacctcg cgcaggtcgg gcgcgaggtg ctgcagcagg
     4621 cgcagcgcct cgtcgcggac atcggcgtgg ttgttgtgca gggccccggc gcggccgctg
     4681 agccaccacg agaacgccga cttgacgcgg ttgcgccgga accggtggcg cagcaggttg
     4741 tgcgtggtgt tggtggtgat gcgataaagc caggtgctca gcgtcgagtt ggccttgaag
     4801 ctcgccagcg cagcgaaggc gcgggcgaag atcgtctgga cgaggtcgtc gatctccgag
     4861 tccggtccga gcaggcgata gagatggccg cggacctgcg actggtgcgt gtcgtagaac
     4921 tgacgcagcg cccgcgggtt gcccgcccgc gcccgcgcca gcatgacctg ctcctccgcg
     4981 gtgaggtagt cgccggcccg cgcgccgtcc tcggcgacgt cgggtacgtc ggccgcgagg
     5041 tcgatcgcct gcgtcagctg cgtcagctgc atcatccgat ttgttgggcg ctccacggcg
     5101 ttcgtcaacc ttctcgcgaa ccgagggacc agcataccga tctctcgcgc gctggcgagg
     5161 gctacactca ccttcatggc agcagacgcc ttgaccgcgt ggcagaccgc atacgcccgg
     5221 cggccggtga ccccggaggc gtgcgcgtcc atggccgaga agtacgcggc gctcgccgcc
     5281 ctcgtcgcgc tgcccgcggg cc
//
LOCUS       DASWNJ010001103         1921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_585232, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001103 DASWNJ010000000
VERSION     DASWNJ010001103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585232"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="VGB85_18520"
     CDS             complement(<1..1040)
                     /locus_tag="VGB85_18520"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0136091.1"
                     /translation="MTASLSTPPAPFDLLSALAEVAGRPAAARALARRLGADELLLLV
                     RDPELGVLLPAPGFPQTMRGGPTWRTFLATCDVPGQHRGSVQRRDGEPPVPVLGHVGP
                     DGVTLVLIGGAPDLAEVEALLPALPLLGALLRAETAARIAEGAAAAARDAGRQARDLT
                     RALDTTRSDLEGALRESARLNHELQDADRRKDEFLAMLGHELRNPMAAITSALELMRV
                     SPDSLEVLGRARAVIERQSQQLGHLIDDLLDVARVTRGKIVLRSVPVDLNEVVRRAVE
                     TTHSLVMSRKHRLDAQYAAPLLVLGDPTRLEQIATNLLSNAAKYTDECGKITVAVARE
                     GDEAVLRVQDSG"
     gene            complement(1044..>1921)
                     /locus_tag="VGB85_18525"
     CDS             complement(1044..>1921)
                     /locus_tag="VGB85_18525"
                     /inference="COORDINATES: protein motif:HMM:NF021926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEY0136092.1"
                     /translation="GRGIGADRQKAAQELVGAFHGTAIKTHPYRSALIMTDALAGYAD
                     DLVEQLTIATAGQYQFFGGGAGDDAQFRRTTVFFGDEALTDAVVALEMLSTKPLGIGV
                     GHGWKPASAAMRVTEARGVTLVSLNGLPVVDAFEAHAEATGQTFDRELPLPFFLHNIL
                     GIDTGSGHRLRVPLAVGADGSVTCASEIPVGARVHIMATDVDSTVESARRATESAIAA
                     LQGMKPRVALFFDCVATRLRMGEAFGHELDALGQLLGPAVTYLGCNTHGQIARAEGQF
                     GGFHNCTAVVCVLPE"
BASE COUNT          256 a          632 c          780 g          253 t
ORIGIN      
        1 atgccgctgt cctgcacgcg cagcacggcc tcgtcgccct cacgcgcgac cgcgacggtg
       61 atcttgccgc actcatcggt gtacttggcg gcgttgctga gcaggttggt ggcgatctgc
      121 tcgaggcgcg tcgggtcgcc gaggaccagc agcggggccg cgtactgggc gtcgaggcgg
      181 tgcttcctcg acatgacgag cgagtgggtg gtctcgacgg cgcggcgcac gacctcgttg
      241 aggtcgacgg gcacgctgcg cagcacgatc ttcccgcggg tgacgcgggc gacgtcgagc
      301 aggtcgtcga tcaggtggcc gagctgctgc gactggcgct cgatcaccgc gcgcgcgcgg
      361 ccgaggacct cgagcgaatc ggggctgacg cgcatcagct cgagcgcgct ggtgatcgcc
      421 gccatcggat tgcgcagctc gtggcccagc atcgccagga actcgtcctt gcggcggtcg
      481 gcgtcctgca gctcgtggtt gaggcgggct gactcgcgta gcgcgccctc gaggtcgctg
      541 cgcgtggtgt cgagggcgcg ggtcaggtcg cgcgcctgcc ggccggcgtc gcgcgcggcc
      601 gcggcggcgc cctcggcgat gcgggcggcg gtctcggccc gcagcagcgc gccgagcagc
      661 ggcaacgcgg gcagcagcgc ctcgacctcg gcgaggtccg gcgcgccacc gatcagcacg
      721 agggtcacac cgtcggggcc gacgtggccg agcaccggga ccggcggctc gccgtcgcgg
      781 cgttgcacgc tgccgcggtg ctggccgggc acgtcgcagg tcgccaggaa ggtgcgccag
      841 gtcgggccgc cgcgcatggt ctgcgggaag ccgggggcgg gcaggaggac gccgagctcg
      901 gggtcacgga ccagcagcag cagctcgtcg gcgccgagcc ggcgggcgag cgcacgcgcg
      961 gcggccggtc gtccggcgac ctcggcgagc gccgagagca ggtcgaacgg cgcaggcggc
     1021 gtggacaggg acgcggtcac gattcactcg ggcagcacgc acacgacggc ggtgcagttg
     1081 tggaacccgc cgaactgccc ctccgcgcgc gcgatctgcc cgtgcgtgtt gcagccgagg
     1141 taggtgaccg cggggccgag cagctggccg agggcgtcga gctcgtggcc gaaggcctcg
     1201 cccatgcgca ggcgggtggc gacgcagtcg aagaacaggg cgacccgcgg cttcatgccc
     1261 tgcagcgcag cgatcgcgga ctcggtcgcg cggcgggcgg actcgacggt cgagtcgacg
     1321 tcggtcgcca tgatgtgcac gcgggcgccg acggggatct ccgaggcgca ggtgaccgag
     1381 ccgtccgcgc cgaccgccag cggcacgcgc aggcgatgac cgctgccggt gtcgatgccg
     1441 aggatgttgt gcaagaagaa cggcagcggc aactcgcggt caaaggtctg gccggtcgcc
     1501 tcggcgtggg cctcgaaggc gtcgacgacg ggcaggccgt tgaggctgac gagggtgacg
     1561 ccgcgggcct cggtgacgcg catggcggcg ctggcgggct tccagccgtg gccgacgccg
     1621 atgccgagtg gtttggtcga gagcatctcg agcgcgacga cggcgtcggt gagggcctcg
     1681 tcgccgaaga agacggtggt gcggcgaaac tgggcgtcgt caccggcgcc accgccgaag
     1741 aactggtact ggccggcggt ggcgatcgtc agctgctcga cgaggtcgtc ggcgtaaccg
     1801 gcgagcgcgt cggtcatgat cagcgcggag cggtaggggt gggtcttgat cgcggtgccg
     1861 tggaaggctc cgacgagctc ctgcgcggcc ttctggcggt cggcaccgat gccgcggccg
     1921 a
//
LOCUS       DASWNJ010001104         2110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_443520, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001104 DASWNJ010000000
VERSION     DASWNJ010001104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443520"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VGB85_18530"
     CDS             complement(<1..198)
                     /locus_tag="VGB85_18530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136093.1"
                     /translation="MTDAAKLKLVSPQGASPEAAEKPAPRKPRSAVDQPLAINGGPAV
                     RRTPLPGPYPGALLMGREEEKG"
     gene            complement(283..1026)
                     /locus_tag="VGB85_18535"
     CDS             complement(283..1026)
                     /locus_tag="VGB85_18535"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HEY0136094.1"
                     /translation="MAAGEGTRLHPHTLDRPKCLVEVGGRPLLDRALAALEAVGFQEV
                     VIVTGYREDVLQAFLAGRKHGLRVTCVRNPEYARTNNAYSLWSAREAVPGDFVLLDGD
                     LLFEPAVLTALLATAGEAALAVERRADLGDEEMKVLVGPDVSVAAVNKTMDPGAAIGE
                     SVGVARFSAMAARALWDCLGAQIEQGQRNVFYELAFEELIADGWRFEIGDVTGLACME
                     IDTPQDLEAAHELALRIDRAPPSAVPSAS"
     gene            complement(1231..1500)
                     /locus_tag="VGB85_18540"
     CDS             complement(1231..1500)
                     /locus_tag="VGB85_18540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEY0136095.1"
                     /translation="MISTREEVLAVVRRHLADTVEGIEGKEIDPSKSMKELGANSLDI
                     VEVVSCSMRELKVKVPRAELNKLTNIDELVDLLHRSVQEKANAAS"
     gene            complement(1497..>2110)
                     /locus_tag="VGB85_18545"
     CDS             complement(1497..>2110)
                     /locus_tag="VGB85_18545"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0136096.1"
                     /translation="MVKQDHDAVEAIISNVSFAHVSKSHEDLDKKSFLRSLGYSAWPF
                     VGYLQSAHAAFGRYPRYAIGMSSRGPEYFLPGYDFVAASKTVMETYCRYLTADLLSED
                     IRINVVRANPVETRSLEATFGPEFAPFCKKYYGEGFFVTAEEVAASALGLCSGLMDAV
                     KGQVLLLDRGFGFADNVVRLFSEREQHGLSVLTPQDRLVKENK"
BASE COUNT          332 a          670 c          750 g          358 t
ORIGIN      
        1 gcccttctcc tcctcgcggc ccatcagcag ggcgccgggg tacgggcccg gcaggggggt
       61 cctccgcacc gcggggccgc cgttgatcgc gagcggctgg tcgacggcgc tgcggggctt
      121 gcggggcgcg ggcttttcgg ccgcttcggg ggaggcgcct tgcggcgaga cgagcttgag
      181 cttggcggcg tcggtcatac gtgcgggccc ttcgatcgcg gcggaaaatg actcggaaaa
      241 tgactcggaa aatgactcgg aaaatgactc gatggatggc aactatgaag cggagggtac
      301 ggcgctcggg ggcgcccgat cgatgcgcag ggcgagctcg tgggcggcct cgaggtcctg
      361 cggcgtgtcg atctccatgc aggcgaggcc ggtgacgtcg ccgatctcga agcgccagcc
      421 gtcggcgatc agctcctcga aggccagctc gtagaagaca ttgcgctggc cctgctcgat
      481 ctgcgcgccg aggcagtccc acagggcgcg ggcggccatc gccgagaagc gggcgacgcc
      541 gactgactcg ccgatcgcgg cgccggggtc catcgtcttg ttcacggcgg cgacggagac
      601 gtcgggtccg acgaggacct tcatctcctc gtcgccgagg tcggcgcggc gctcgaccgc
      661 cagcgccgcc tcacccgccg tggcgagcag cgccgtcagc accgcgggct cgaacagcag
      721 gtcgccgtcg agcagcacga agtcgccggg caccgcctcg cgcgcgctcc acagcgagta
      781 cgcgttgttg gtccgcgcgt actcggggtt gcgcacgcag gtgacgcgca ggccatgctt
      841 gcggccggcg aggaaggcct ggagcacgtc ctcgcggtag ccggtgacga tgacgacctc
      901 ctggaagccg accgcctcga gcgccgcgag ggcgcggtcg agcagcgggc gaccgccgac
      961 ctcgacgagg cacttggggc gatcgagcgt gtgcgggtgc aggcgagtgc cttccccggc
     1021 ggccaatagg accacggtac tcacggtcga cgcgggttcc aagccaggag tcattaagct
     1081 tgaagatcgg gcttgtcaaa tacggccggg ggcacgacac cacgccgtga gagcccgccg
     1141 tgtgcatctc gtttcacgga ggatgccacg gcggttcacg gtgcgtagcc tgcttcacag
     1201 gcggggtggt agcgacgccg gacgcggcgt ttagctcgcg gcgttggcct tctcttgcac
     1261 gctgcggtgc aggaggtcga cgagctcgtc gatgttggtg agcttgttga gctcggcgcg
     1321 gggcaccttg accttgagct cgcgcatgct gcacgagacg acctcgacga tgtcgaggct
     1381 gttggccccg agctccttca tcgacttcga cggatcgatc tccttgccct cgatcccctc
     1441 gacggtgtcg gcaaggtggc gcctgacgac ggcgaggact tcttcgcgtg tgctgatcat
     1501 ttgttctcct tcaccaggcg gtcttggggg gtcaaaaccg agaggccgtg ctgctctcgc
     1561 tccgagaaca ggcggacgac gttgtccgcg aaaccgaagc ccctgtccag tagtaggacc
     1621 tgtcccttga ccgcgtccat gagtccgctg cacagcccca gcgcggaggc tgcgacttct
     1681 tcggctgtca cgaagaagcc ctccccgtag tacttcttgc agaacggggc aaattccggg
     1741 ccgaacgtgg cttctagcga gcgcgtctcg accgggttgg cgcgcacgac gttgatgcga
     1801 atatcctcac tgagcagatc ggcagtgagg tagcggcaat aggtctccat caccgtcttg
     1861 ctggcggcga cgaagtcata gcccgggagg aagtactccg gcccgcgcga ggacatgccg
     1921 atcgcgtagc gcgggtagcg gccgaaggcc gcgtgagcgc tctgcaaata gcccacgaag
     1981 ggccacgcgc tgtagccgag gctgcgcagg aagctctttt tgtcgaggtc ctcgtgcgac
     2041 ttgctgacgt gggcgaacga cacgttgctg atgatcgcct cgacggcgtc gtggtcctgc
     2101 ttgaccatcg
//
LOCUS       DASWNJ010001105        12568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_125103, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001105 DASWNJ010000000
VERSION     DASWNJ010001105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 12568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12568
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125103"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VGB85_18550"
     CDS             complement(<1..487)
                     /locus_tag="VGB85_18550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136097.1"
                     /translation="MRPHHLLSVALLLVACAHRDPYSTTDSTLGLDRVVLYRNGVGYF
                     ERSGEVDGDVLTIKVRKDQVNDLLKSLTVVDRRNGQAVSVSMPLDPQVWASAALATLS
                     PGQGSLDQILDALRGNEVVLGTTQGKIRGRIVMIEPIVDEPDASPPPHSERVMPPQAG
                     SK"
     gene            720..1685
                     /gene="pip"
                     /locus_tag="VGB85_18555"
     CDS             720..1685
                     /gene="pip"
                     /locus_tag="VGB85_18555"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEY0136098.1"
                     /translation="MVPTRRALYPEIEPYRTGSLQVSGLHCIYYEESGNPEGKPVVFL
                     HGGPGGGTDPKQRRFFDPEAYRIVLFDQRGCGKSTPHAELRENTTWDLVADIEKLRVH
                     LGIERWQVFGGSWGSTLALAYAQTHPKRVSELVLRGIFLLRRAELLWFYQDGASALFP
                     DAWEHFLAPIPVAERGDLMRAYYRRLTGDDEDVRREAARAWSVWEASTSFLIQRPDFI
                     AHAAGDDFSLAFARIEAHYFVHHGFLEREQQLLENVGRLRQIPSVIVQGRYDVVCPMH
                     SAWQLHRAWPEAELVVVPDAGHSAFEPGTLAALIDATDRFSVRRD"
     gene            1703..2686
                     /gene="pip"
                     /locus_tag="VGB85_18560"
     CDS             1703..2686
                     /gene="pip"
                     /locus_tag="VGB85_18560"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEY0136099.1"
                     /translation="MADRPDTLPPRADPLSMLYPPIEPYHLGRLAVDARHDLYFEQSG
                     NPNGKPVVFLHGGPGGGTDPKHRRFFDPEHYRIILFDQRGCGQSEPFASLEDNTTWHL
                     VDDIEALRVHLGIEAWQVFGGSWGSTLAVAYAEAHPDRVTDLVLRGIFMFTTREMEWF
                     YGHGTRMLFPDAWEAFVAIIPESERENLVAAYYTRLTGDDAQVRAEAARAWSLWESRV
                     ATLIADESTLAHCEDPGFTLAFARIECHYFLHGGFLAHETQLLDDAEKIRHIPTTIVH
                     GRYDVICPVENAWRLHRCLPQSELVLVDDGGHSAFEPGVAAALRAATDRHR"
     gene            2733..3857
                     /locus_tag="VGB85_18565"
     CDS             2733..3857
                     /locus_tag="VGB85_18565"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY0136100.1"
                     /translation="MSLRPILALAVLLLSACGHGSSDGATQTSPSQLAKIKLPPGFEI
                     AVYAEVPAARSMTLGADGTVFVGTRADDKVYAVRDTDGDHVAETVQVIASGLDTPNGV
                     AYRNGDLYIAEISRVIKLPGIDGKLADPPKPVVVRDDFPSDTHHGWKYIAFGPDDKLY
                     VPVGAPCNVCLKDDPRYSSITRMNADGGEFEVFAAGIRNTVGFAWHPSTRALWFTDNG
                     RDELGENIPGDELNLAAKPGMHFGFPFCHQGDTPDPEHGKTRRCDEFAPPARVLGPHV
                     AALGMKFYTGTMFPPEYRRQIFIAEHGSWNRTAPLGYRVMLVRLEGERTTSYEVFAEG
                     WLGADGEPWGRPVDILVMPDGALLVSDDKGGKLYRISHRG"
     gene            complement(3862..5097)
                     /locus_tag="VGB85_18570"
     CDS             complement(3862..5097)
                     /locus_tag="VGB85_18570"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEY0136101.1"
                     /translation="MKRVEFWFDFSCPYAYVASTRIEALAERAGAELDARPMLLGGVF
                     KARGTPQNLSATLHPAKAAHNMNDMRRQAQMAGVPLHMPAGHPLRTVEALRCVLAVGA
                     PFMPLAHRFYRAYWAQGRDIGQGAVIDEILREAGLGVREVRSRAASHEIKDELRRRTD
                     EAVEREIFGVPTCFVGDELFWGNDRLDMVEAALGGTPASPRPDPGAALAPVDFWFDYS
                     SPFAYLALAQAEAVLGASATWRPLLLGGLFRSVGTPDVPLFTQSPEKRRHTMQDLTRQ
                     AERLGVEFSWPTRFPVSTVLALRVTLQARLAETPEGRALIVRIARALWAEDRDIADPV
                     VLAGLADEMGFRGDELVAAASEGKAALFAATSAAEAAGVFGVPSFVVHPPGKEPLLYW
                     GVDRMDWAARAASGDETVM"
     gene            complement(5100..6086)
                     /gene="hemB"
                     /locus_tag="VGB85_18575"
     CDS             complement(5100..6086)
                     /gene="hemB"
                     /locus_tag="VGB85_18575"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEY0136102.1"
                     /translation="MHSILPHRPRRNRKHPAIRAMIRERSVGPEHFIYPLFIHAGDAD
                     IPIGSMPGCSRLSAAGLLREVEAARAVGVHSFALFPAVAEGLKTPDGREAHNPDGLIP
                     RTLRILKDRWPDVVLVTDVALDPYNSDGHDGIVGKDGRILNDETVAVLCKQALTQARA
                     GADIVAPSDMMDGRVGAIRITLDQEGFHDVSILAYTAKYASAFYGPFREALDSAPKSG
                     DKKTYQMDPANSREALREAELDQAEGADMLMVKPAGPYLDIIRRLRESTHLPIAAYQV
                     SGEYAMLKAASERGWLDEKKAALESLLGIRRAGADLILTYYARQAAQWLAGE"
     gene            complement(6108..6530)
                     /locus_tag="VGB85_18580"
     CDS             complement(6108..6530)
                     /locus_tag="VGB85_18580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEY0136103.1"
                     /translation="MPSVFTRIIQGELPGRFLWKDELCVVFLSIAPLRPGHALVVPRA
                     EVDHWIDLPPETATHLMAVAQVVGKAQQTAFNPARIGLMIAGFEVPHVHVHVVPTMGM
                     RDLDFANADGRAAAASLDAAADRLRAALRAQGGTHVAD"
     gene            complement(6560..7231)
                     /locus_tag="VGB85_18585"
     CDS             complement(6560..7231)
                     /locus_tag="VGB85_18585"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEY0136104.1"
                     /translation="MPAAKRSSSTMRADTGAGVASVAATGALMPRSFYARDALLVARD
                     LLGKLLIRDEVVLRITEVEAYRHPNDTANHCRAGRTERNAAMWGPGGRAYMYLCYGMH
                     NLLNLVTGLEGEGAAVLIRACEPVAGLPQIQRRRKLESDRPTLLAGPGKVGQALGLDP
                     TWSHHPLYEPGGLTLHDAPPVAAILAGPRVGIDYAQPADRVAPWRLAVAGTAWVTVPG
                     TLRPV"
     gene            7244..7927
                     /locus_tag="VGB85_18590"
     CDS             7244..7927
                     /locus_tag="VGB85_18590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136105.1"
                     /translation="MACSRASRKPLHLACGRVIIFPPRMYVYLYLFCFALGGLLLLAS
                     ILLGDKDGGHDTGDTDTEGETGGTAGTQGIDHGVPASPGSIAGVFTAFLSMRFWMFFL
                     AFFGLTGLVLDGLDLVPDSHVALVISLAMGLATGQATAFVFRHLATSETSTAAGVGDY
                     VGKSGRVLVGFGPGDLGKLRLTLKGTTVDVLATSDEERPFTPGEEGLVIQMNDTTAVV
                     TRVPGRADT"
     gene            8070..9467
                     /locus_tag="VGB85_18595"
     CDS             8070..9467
                     /locus_tag="VGB85_18595"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY0136106.1"
                     /translation="MHLAVVFTPLLGRAPGLEILTQDMATFAVVGAVAVALFILLLLL
                     LVKNYLHIARPNEALIFTGRRRMRDGVDLGPMIVLGRGDTRQAAEHHGGGRGRAWRVP
                     IIERVDRLDMAVMSIDVAVHNAYSAGNIPLRIHAIANVKISSDPRLIRNAIERFLGQS
                     QQEIMTVARQTLEGALREVLAQMTPEGVNEDRLAFADNLVKSAKDDLDKLGLQLDTLK
                     IQHVSDETGYLDSLGRPQIAAVLRDAEKAESQASQEVSEAQAAANQRAEVAKATAETA
                     ILMKRNALQQLRSELEGQASSVEREAEAAAKTARAAAEQDLQTVRGQLEERRLMAEVI
                     IPAEAHRAAEAILAKGAAAPTIEHGAAVAEVLQLTGEAWRSMGPQAKEIYVIQHLEEL
                     ISAVVESVDKIKVREVHVLDPGDGSGIASYASTFPHTVAAVLSALRETTGVDIPAILA
                     GSPSAALATKSTRTP"
     gene            9464..10759
                     /locus_tag="VGB85_18600"
     CDS             9464..10759
                     /locus_tag="VGB85_18600"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY0136107.1"
                     /translation="MNTDVIFALLGVGLVAFVALLLVIKNVLYVCQPNEVLIFSGRQR
                     RGADGKLYGYRIIRGGRAIRIPLFETVDRMDLTNMIIEVQVKNAYSKGGIPLNIQGFA
                     NIKVPGEEPLLNNTLERFLGRGREQIMKTARETLEGNLRGVLSTMTPEQVNTDKETFA
                     QRLAAEAEHDLSKLGLVLDTLKIQNVSDEVGYLDAMGRQRSAAIRRDALIAEAVSQAQ
                     AAEWRWANTRNAEVATLESQMQIAAKENERRVADAQSLRGALIAQQQAEVQALIAQAT
                     GEVAMQEARIEQVRRRLHADVIQPAEAARRQAEEQAKGAASLVIEQGRATASVLARIA
                     ENYNKGGTSSRDVLLMQKLVPLFQRLAGTIGDLKIDRLTILPHRTGDGGGAGLGARLV
                     DANEQVRAVTGVDLGKVLRDKLGDDDLRPTSRPPPPPSR"
     gene            11072..11683
                     /locus_tag="VGB85_18605"
     CDS             11072..11683
                     /locus_tag="VGB85_18605"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0136108.1"
                     /translation="MRDAVREESRAAYREAILSAAERAFGRLGFRDAKMADIAAEAGV
                     AAGTLYNYFKNKDEVFASILQRGQEQVGALFESHDGVDDPLERTRLRLRDAFAYLEEH
                     GVLFSMFLRLGGFIEHAQKRVLEDHSEFGYTRGVERTTAILREAVARGQVRSDVAVEE
                     LAVMLMGACDATILAWTRAGCPPGLVDKADIIIELFIRGAQAP"
     gene            11680..>12568
                     /locus_tag="VGB85_18610"
     CDS             11680..>12568
                     /locus_tag="VGB85_18610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0136109.1"
                     /translation="MTTASTPRPFALSSRLLLAASLLTGVACDSGEEKKPGLPPAKDG
                     ASVPAIPGPPPKSEVGPAGAGASASASTGVAPVTARYVATALPKHSAELGPRMSGTLT
                     AVLVEEGDHVKKGQQLFRLDSRNSRLGVEQAEAGLQGAAIARDNAQRELERQRVLAEK
                     GTISAAVLERAEATFNSTVNGVKQAEVMLSMAKRSTSDSAVVSPIDGVVAKKLKSVGE
                     TVTMMPPTTVVLIQDHSVIELRTRIPESTLKHVKEGELITAHFTAIDATREARVVRIQ
                     PTVDPMTRTIEIVADVDNKD"
BASE COUNT         1929 a         4780 c         4085 g         1774 t
ORIGIN      
        1 ccttggaccc ggcctgcggc ggcatcacgc gctcgctgtg cggcggcggc gaggcgtcgg
       61 gctcgtcgac gatcggctcg atcatcacga tgcgcccgcg gatcttgccc tgcgtcgtgc
      121 cgagcacgac ctcgttgccg cgcagcgcgt cgaggatctg gtcgagcgag ccttgccccg
      181 gggacagggt cgccagcgcc gcgctcgccc acacctgcgg gtcgagcggc atcgacaccg
      241 agaccgcctg accgttgcgg cggtcgacca ccgtcaggct cttgagcagg tcgttgacct
      301 ggtccttgcg caccttgatc gtcaggacgt cgccgtcgac ctcgccgctg cgctcgaagt
      361 agccgacgcc gttgcgatac agcaccaccc ggtcgagccc gagcgtgctg tcggtcgtcg
      421 agtaggggtc gcgatgggcg caggcgacga ggagcaaggc gacggaaagc aggtggtgtg
      481 ggcgcaaggg caagatctcc taccggcgtc gactgtaccc cggggccgtc tcgcaggatc
      541 gcggggcccg ccggcacgag aggcgagcaa agccggctgc gaggccccgc gcgcggccct
      601 gtgcctgtgc cgcgcgttcg cgcggcttcg cgcagatcac gccaacgcag tgactggagc
      661 catacaccag cagaccgctc ggtagacgcg atcgctcgcg tttgccaatg tagcccgcga
      721 tggtccccac ccgtcgcgcg ctctacccgg agatcgagcc ctatcgaacc ggctccctgc
      781 aggtctcggg cctgcattgc atctactacg aggagtccgg caaccccgag ggcaagccgg
      841 tcgtgttcct gcacggcggc cccggtggcg gcacggaccc caagcagcgc cgcttcttcg
      901 accccgaggc ctatcgcatc gtgctgttcg atcaacgcgg ctgcggcaag agcactccgc
      961 acgccgagct gcgcgagaac accacctggg acctcgtcgc cgacatcgag aagttgcgcg
     1021 tgcacctcgg catcgagcgc tggcaggtgt tcggcggctc gtggggcagc acgctcgcgc
     1081 tcgcctacgc gcagacccac ccgaagcgag tcagcgagct cgtgctgcgc gggatcttcc
     1141 tgctgcgccg cgccgagctg ctgtggttct accaggacgg cgccagcgcc ctgttccccg
     1201 acgcgtggga gcacttcctc gcgccgatcc ccgtcgccga gcgcggcgac ctgatgcggg
     1261 cctactaccg ccgcctcacc ggcgacgacg aggacgtgcg ccgcgaggcc gcgcgcgcgt
     1321 ggagcgtgtg ggaggcgagc accagcttcc tgatccagcg cccggacttc atcgcccacg
     1381 ccgccggcga cgacttctcc ctcgccttcg cgcgcatcga ggcccactac ttcgtgcatc
     1441 acggcttcct cgagcgcgag caacagctgc tcgagaacgt cggccgcctg cgccagatcc
     1501 cgagcgtcat cgtgcagggt cgctacgacg tcgtgtgccc gatgcacagc gcgtggcagc
     1561 tgcaccgcgc gtggcccgag gccgagctcg tcgtcgtccc cgacgccggc cactccgcct
     1621 tcgagcccgg cacgctcgcc gccctcatcg acgccaccga ccgcttcagc gtccgccgcg
     1681 actgacaccc ccgagcttcg ccatggccga ccgacccgac accctccccc cgcgcgccga
     1741 cccgctgagc atgctctacc cgccgatcga gccgtaccac ctcggtcggc tcgccgtcga
     1801 cgcgcgccac gacctctact tcgagcagtc cggcaacccg aacggcaagc cggtcgtgtt
     1861 cctgcacggc ggacccggcg gcggcacgga ccccaagcac cgccgcttct tcgaccccga
     1921 gcactaccgc atcatcctgt tcgaccagcg cggctgcggc cagagcgagc ccttcgcctc
     1981 gctcgaggac aacaccacct ggcacctcgt cgacgacatc gaggcgctgc gcgtgcacct
     2041 cggcatcgag gcctggcagg tgttcggcgg ctcgtggggc agcacgctcg cggtcgccta
     2101 cgccgaggcc caccccgacc gcgtcaccga cctcgtcctg cgcgggatct tcatgttcac
     2161 cacccgcgag atggagtggt tctacggcca cggcacccgc atgctgttcc cggacgcgtg
     2221 ggaggccttc gtggcgatca tccccgagtc cgagcgggag aacctcgtcg ccgcctacta
     2281 cacgcgcctg accggcgacg acgcgcaggt ccgggccgag gccgcgcgcg cgtggagcct
     2341 gtgggagagc cgcgtcgcca ccctgatcgc cgacgagtcg accctcgccc actgcgagga
     2401 ccccggcttc accctcgcct tcgcgcgcat cgagtgccac tacttcctgc acggcggctt
     2461 cttggcgcac gagacccagc tgctcgacga cgccgagaag atccgccaca tcccgaccac
     2521 gatcgtgcac ggccgctacg acgtcatctg cccggtcgag aacgcgtggc gcctgcacag
     2581 gtgcctgccg cagtccgagc tcgtgctcgt cgacgacggc ggacactccg cgttcgagcc
     2641 cggggtcgcc gcggccctgc gcgccgccac cgaccgccat cgctgacgcc cggcgtttgc
     2701 aggcggccgc gcgcgcgata tggttcgcgc caatgtcgct gcgcccgatc ctcgccctcg
     2761 ccgtcctcct gctgagcgcg tgcggacacg gcagctccga cggcgcgacc cagaccagcc
     2821 cgtcgcagct ggcgaagatc aagctcccgc ccggcttcga gatcgccgtc tacgccgagg
     2881 tgcccgcggc gcgctcgatg accctcgggg ccgacggcac cgtgttcgtc ggcacccgcg
     2941 cggacgacaa ggtctacgcc gtgcgcgaca ccgacggtga ccacgtcgcc gagaccgtgc
     3001 aggtgatcgc gtccggcctc gacactccca acggcgtcgc ctaccgcaac ggcgacctct
     3061 acatcgccga gatctcgcgc gttattaaat tacccggcat cgacggcaag ctcgccgatc
     3121 cgccgaagcc cgtcgtcgtg cgcgacgact tcccctcgga cacccaccac ggctggaagt
     3181 acatcgcctt cggccccgac gacaagctct acgtcccggt cggcgcgccg tgcaacgtgt
     3241 gcctcaagga cgacccgcgc tactcctcga tcacgcgcat gaacgcggac ggcggcgaat
     3301 tcgaggtgtt cgcggctggg atccgcaaca ccgtcggctt cgcctggcac ccgagcacca
     3361 gggcgctgtg gttcactgac aacggccgcg acgagctcgg cgagaacatc cccggcgacg
     3421 agctcaacct ggccgcgaag ccaggcatgc acttcggctt cccgttttgc catcagggcg
     3481 acaccccgga ccccgagcac ggcaagaccc gcaggtgcga cgagttcgcg ccgccggccc
     3541 gcgtgctcgg cccgcacgtc gccgcgctcg gcatgaagtt ctacaccggc acgatgttcc
     3601 cgccggagta ccgccgccag atcttcatcg ccgagcacgg ctcgtggaac cgcaccgcgc
     3661 cgctcggcta ccgcgtcatg ctcgtgcgcc tcgagggcga gcgcaccact tcctatgaag
     3721 tgttcgcgga gggctggctc ggcgccgacg gtgaaccctg gggccgcccc gtcgacatcc
     3781 tcgtgatgcc cgacggcgcc ctgctcgtct cggacgacaa gggcggcaaa ttgtatcgca
     3841 tcagccaccg cggctgacgc gtcacatcac tgtctcgtcg cccgaggccg cccgtgcggc
     3901 ccagtccata cggtcgacgc cccagtacag cagcggctcc ttaccgggcg gatgcacgac
     3961 gaagctcggc accccgaaca cccccgcggc ctcggccgcg ctcgtcgcag cgaacagcgc
     4021 cgccttgccc tcgctcgccg ccgcgaccag ctcgtcgccg cgaaagccca tctcgtccgc
     4081 caggccggcg agcaccaccg gatcggcgat gtcgcgatct tcggcccaca gcgcgcgcgc
     4141 gatgcgtacg atcagcgcgc ggccctcggg cgtctcggcg aggcgcgctt gcagcgtcac
     4201 gcgcagcgcc agcaccgtgc tgaccgggaa gcgcgtcggc cacgagaact cgacgccgag
     4261 acgctcggcc tggcgagtca ggtcctgcat cgtgtgccgg cgcttctccg ggctctgcgt
     4321 gaacagcggg acatccggcg tgccgacgct gcgaaacagc ccgccgagca gcagcggccg
     4381 ccacgtcgcc gacgccccga gcaccgcctc ggcctgcgcg agcgcgaggt aggcgaaggg
     4441 gctcgagtag tcgaaccaga agtcgaccgg cgccagcgct gcgcccgggt cgggccgcgg
     4501 cgacgccggt gtgccgccga gcgccgcctc gaccatgtcg agccggtcgt tcccccagaa
     4561 cagctcgtcg ccgacgaagc aggtcggcac cccgaagatc tcgcgctcga ccgcctcgtc
     4621 ggtgcgccgg cgcagctcgt ctttgatctc atgactcgcg gcccgtgacc gcacctcacg
     4681 gacaccgagc cccgcctcgc gcaggatctc gtcgatcacc gcgccttgcc cgatgtcccg
     4741 cccctgcgcc cagtacgcgc gatagaagcg gtgcgccagc ggcatgaacg gcgcgccgac
     4801 ggcgagcaca cagcgcagcg cctcgaccgt gcgcagcgga tgccccgcgg gcatgtgcag
     4861 cggcaccccg gccatctgcg cctggcgccg catgtcgttc atgttgtgcg ccgccttggc
     4921 cgggtggagc gtcgccgaca ggttctgtgg agttccccgc gctttaaaaa ctcccccgag
     4981 cagcatcggc cgcgcgtcga gctcggcccc ggcgcgctcc gccagcgcct cgatccgcgt
     5041 cgaggcgacg tacgcgtagg gacacgagaa gtcgaaccaa aactcgacgc gcttcatcgt
     5101 cactccccgg ccagccactg cgcggcctgg cgcgcgtagt acgtcaagat caggtccgcc
     5161 ccggcgcggc ggatgccgag caggctctcg agcgccgcct tcttctcgtc gagccagccg
     5221 cgctcgctcg ccgccttgag catcgcgtac tcgccgctga cctggtacgc cgcgatcggc
     5281 aggtgcgtcg actcgcgcag gcggcggatg atgtcgaggt acggccccgc cggcttgacc
     5341 atcagcatgt cggcgccctc ggcctggtcg agctcggcct ctcgaagcgc ctcgcggctg
     5401 ttggccgggt ccatctggta ggtctttttg tcgccgctct tgggcgccga gtcgagcgcc
     5461 tcgcgaaacg gcccgtagaa cgccgacgcg tacttggccg tgtacgccag gatcgacacg
     5521 tcatgaaagc cctcctggtc gagggtgatg cggatcgcgc cgacgcggcc gtccatcatg
     5581 tcgctcggcg cgacgatgtc ggcccccgcc cgcgcctggg tgagcgcctg cttgcacagc
     5641 accgcgaccg tctcgtcgtt gaggatccgt ccgtccttgc cgacgatgcc gtcgtggccg
     5701 tccgagttgt aggggtcgag cgcgacgtcc gtcaccagca cgacatcggg ccagcggtcc
     5761 ttgaggatcc tgagcgtgcg tgggatcagg ccgtcggggt tgtgcgcctc acgaccgtcg
     5821 ggcgtcttga ggccctcggc gaccgcgggg aacagcgcga agctgtgcac cccgaccgcg
     5881 cgcgccgcct cgacctcgcg cagcagcccc gcggccgaca gccggctgca cccgggcatc
     5941 gacccgatcg ggatgtccgc gtcaccggcg tggatgaaca gcggataaat aaaatgctcg
     6001 gggccgacgc tgcgctcccg gatcatcgcg cggatcgccg ggtgcttgcg gttacgacgg
     6061 ggacggtggg gcaggatcga atgcatgcgt gtgtctctcg gggcaggtca gtcggcgacg
     6121 tgggtgccac cctgcgcgcg cagggccgcg cgcagccggt ccgcggccgc gtcgaggctg
     6181 gccgccgcgg cgcgcccgtc ggcgttggcg aagtcgaggt cacgcatgcc catcgtcggg
     6241 accacgtgca catgcacgtg cgggacctcg aagccggcga tcatcaggcc gatgcgcgcc
     6301 gggttgaacg cggtctgctg ggccttgccg acgacctgcg cgacggccat caagtgcgtc
     6361 gcggtctccg gcggcaggtc gatccagtgg tcgacctcgg cgcgcgggac caccagcgcg
     6421 tgaccgggac gcagcggcgc gatcgacagg aacacgacgc acagctcgtc cttccacagg
     6481 aaccggccgg gaagctcccc ttggatgatg cgggtgaaca cggagggcat gcgcccgatg
     6541 atagtacggt ttggtgcggt tacaccggcc gcagcgtgcc cggaacggtc acccacgcgg
     6601 tacccgcgac cgcgagccgc cagggcgcca cgcggtcggc cggctgcgcg tagtcgatgc
     6661 ccacccgcgg ccccgccaaa atggccgcca ccggcggcgc gtcgtgcagc gtcagcccgc
     6721 ccggctcgta cagcggatgg tgcgaccagg tcgggtcgag cccgagcgcc tggccgacct
     6781 tgcccggacc tgcgagcagg gtcggacggt cgctctccag cttgcgccgg cgctggatct
     6841 gcggcaggcc cgccaccggc tcgcacgccc ggatcaacac cgccgcgccc tccccctcga
     6901 gcccggtcac gaggttgagc aggttgtgca tgccgtaaca cagatacatg taggcgcgcc
     6961 cgcccgggcc ccacatcgcc gcgttgcgct cggtgcgtcc ggcccgacaa tggttcgcgg
     7021 tgtcgttcgg atggcgatac gcctcgacct cggtgatgcg caggaccacc tcgtcgcgga
     7081 tcaacaactt gccgagcaga tcgcgggcga ccagcaaggc atcgcgcgcg tagaacgacc
     7141 gcggcatcag ggccccggtc gccgccaccg aggccacgcc cgcccccgtg tccgcgcgca
     7201 tcgtcgatga actgcgtttc gccgccggca tgtctccgca agcgtggctt gttctcgcgc
     7261 ctcgcgcaaa ccgctgcacc tggcctgcgg gcgtgtcatc atcttccccc cgcgcatgta
     7321 cgtctatttg tacctgttct gcttcgccct cggcggcctg ctgctgctcg cttcgatcct
     7381 gctcggcgac aaggacggcg gacacgacac cggcgacacg gacaccgagg gcgagaccgg
     7441 cggcactgcc ggtacgcagg gcatcgacca cggcgtgccc gccagccccg gcagcatcgc
     7501 cggggtgttc accgccttcc tgtcgatgcg cttctggatg ttcttcctcg cgttcttcgg
     7561 cctgaccggc ctcgtgctcg acggcctcga cctggtcccc gacagccacg tcgcgctcgt
     7621 catctcgctc gccatgggcc tcgccacggg tcaggccacc gcgttcgtgt ttcgccacct
     7681 cgccaccagc gagaccagca ccgccgccgg cgtcggcgac tacgtcggca agtccggccg
     7741 cgtgctcgtc ggcttcggcc ccggcgacct cggcaagctg cgcctgaccc tcaagggcac
     7801 caccgtcgac gtgctcgcca ccagcgacga ggagcgcccc ttcacccccg gcgaggaggg
     7861 cctggtgatc cagatgaacg acaccaccgc cgtggtcacc cgcgtgcccg gccgcgccga
     7921 cacctgactg gtttacacgg ccctccgggc ctgatccgtt tacacggccc tccgggcctg
     7981 atccgtttac acgctccggc tctcatccgc tttacacggc cctcccggcc ctgatcgtcc
     8041 cctccccggc ctcgccagag agaagcccga tgcacctcgc agtcgtcttt acccccctgc
     8101 tgggccgcgc gcccggcctc gagatcttga cgcaggacat ggccaccttc gcggtcgtcg
     8161 gcgccgtcgc ggtcgccctc ttcatcttgc tgctgctctt gctggtcaag aattacctgc
     8221 acatcgcgcg cccgaacgag gccctgatct tcaccgggcg tcggcgcatg cgcgacggcg
     8281 tcgacctcgg gccgatgatc gtgctcggtc gcggcgacac gcggcaggcc gcggagcacc
     8341 acggtggcgg ccgcggtcgc gcctggcggg tcccgatcat cgagcgcgtc gatcgcctcg
     8401 acatggccgt gatgtcgatc gacgtcgcgg tgcacaacgc ctactccgcc ggcaacatcc
     8461 cgctgcggat ccacgcgatc gccaacgtca agatcagctc cgacccgcgc ctgatccgca
     8521 acgcgatcga gcgcttcctc ggccagtcgc agcaggagat catgacggtc gcccgccaga
     8581 ccctcgaggg tgccctgcgc gaggtcctcg cgcagatgac ccccgagggc gtcaacgagg
     8641 accgcctcgc cttcgccgac aacctggtca agagcgccaa ggacgacctc gacaagctcg
     8701 gcctccagct cgacaccctc aagatccagc acgtctccga cgagaccggc tacctcgact
     8761 cgctcggccg cccgcagatc gccgcggtcc tgcgcgacgc cgagaaggcc gagagccagg
     8821 cctcgcagga ggtctccgag gcccaggccg ccgccaacca gcgggccgag gtcgccaagg
     8881 cgaccgccga gaccgcgatc ctgatgaagc gcaacgccct gcagcagctg cgctccgagc
     8941 tcgagggtca ggcctcgtcg gtcgagcgcg aggccgaggc cgccgccaag accgcccgcg
     9001 ccgccgccga gcaggacctg cagaccgtgc gcggccagct cgaggagcgg cgcctgatgg
     9061 ccgaggtgat catcccggcc gaggcccacc gcgccgccga ggcgatcctc gccaagggcg
     9121 ccgcggcccc gacgatcgag cacggcgccg ccgtggccga ggtgctccag ttgaccggcg
     9181 aggcctggcg cagcatgggc ccgcaggcca aggagatcta cgtcatccag cacctcgagg
     9241 agctgatctc cgcggtcgtc gagagcgtcg acaagatcaa ggtccgcgag gtccacgtgc
     9301 tcgaccccgg cgacggctcc ggcatcgcca gctacgcctc gaccttcccc cacaccgtcg
     9361 ccgccgtgct cagcgccctg cgcgagacca ccggcgtcga catccctgcc attttggccg
     9421 gcagtccctc cgccgcgctc gccacgaaat ccacgaggac cccgtgaaca ccgacgtcat
     9481 cttcgccctg ctcggcgtcg gcctcgtcgc cttcgtcgcc ctgctgctcg tcatcaagaa
     9541 cgtcctgtac gtctgccagc ccaacgaggt cctgatcttc agcggccgcc agcggcgcgg
     9601 cgcggacggc aagctgtacg gctaccgcat catccgcggc gggcgtgcga tccggatccc
     9661 gctgttcgag accgtcgatc gcatggacct gaccaacatg atcatcgagg tccaggtcaa
     9721 gaacgcctac tccaagggcg gcatcccgct caacatccag ggcttcgcca acatcaaggt
     9781 ccccggcgag gagccgctgc tcaacaacac cctcgagcgc ttcctgggcc gcggccgcga
     9841 gcagatcatg aagaccgccc gcgagaccct cgagggcaac ctgcgcggcg tgctgtcgac
     9901 gatgaccccc gagcaggtca acaccgacaa ggagaccttc gcccagcggc tcgccgccga
     9961 ggccgagcac gacctgagca agctcggcct cgtgctcgac accctcaaga tccagaacgt
    10021 cagcgacgag gtcggctacc tcgacgcgat gggccgccag cgctccgcgg cgatccgccg
    10081 cgacgccctg atcgcagagg ccgtcagcca ggcccaggcc gccgagtggc gctgggccaa
    10141 cacccgcaac gccgaggtcg ccaccctcga gtcgcagatg cagatcgcgg ccaaggagaa
    10201 cgagcgtcgc gtcgccgatg cccagtcgct ccgcggcgcc ctgatcgccc agcagcaggc
    10261 cgaggtccag gccctgatcg cccaggcgac cggcgaggtc gcgatgcagg aggcccgcat
    10321 cgagcaggtc cgccgccgcc tccacgccga cgtgatccag ccggccgagg ccgcccgccg
    10381 ccaggccgag gagcaggcca agggcgccgc ctcgctggtg atcgagcagg gccgcgcgac
    10441 cgcctcggtg ctcgcacgca tcgccgagaa ctacaacaag ggcggcacca gcagccgtga
    10501 cgtgctgctg atgcagaagc tcgtcccgct gttccagcgc ctcgccggca cgatcggcga
    10561 cctcaagatc gaccgcctga cgatcctgcc ccatcggaca ggtgatggcg gcggcgccgg
    10621 cctcggcgcg cgcctcgtcg acgccaacga gcaggtccgg gccgtcaccg gcgtcgacct
    10681 cggcaaggtc ctgcgcgaca agctcggcga tgacgacctg cgtccgacgt cgcgcccgcc
    10741 cccgcccccg tcgcgctgag atcctcgcgc gctcgcaccc ccatcgaggg cccggccgac
    10801 ggccgtggcc ctcgatgcgc ggttacgcac cgtgaattac gcttcagacc catgaaccgg
    10861 gcgcaatcga ggccccctat gaaacaggtt tcactgggtg ccgacaaaac gcgtaacaat
    10921 ctatattcat tggatttatt ggatgtgaaa ctaatctcac cttgacatga atcgccgttc
    10981 atcgcaatga acccccgcat tggcgagatc agcggagaca gcgcggggtg gcggagcgaa
    11041 gcggggcgcg cccgggccgg acaagcccag cgtgcgcgac gccgtgcgcg aggagagccg
    11101 ggccgcgtac cgcgaggcga tcctcagcgc ggccgagcga gcgttcggcc gcctcggctt
    11161 tcgcgacgcc aagatggccg acatcgccgc cgaggccggc gtcgccgcgg gcacgctcta
    11221 caactatttt aaaaacaagg acgaggtgtt cgcctcgatc ttgcagcgcg gccaggagca
    11281 ggtcggcgcg ctgttcgagt cgcacgacgg cgtcgacgac ccgctcgagc gcactcgcct
    11341 gcgcctgcgc gacgccttcg cgtatctcga ggagcacggc gtgttgttct cgatgttcct
    11401 gcggctcggc ggcttcatcg agcacgccca gaagcgtgtg ctcgaggacc acagcgagtt
    11461 cggctacacc cgcggcgtcg agcggacgac cgcgatcctg cgtgaggccg tcgcccgcgg
    11521 tcaggtgcgc tccgacgtcg cagtcgagga gctcgccgtg atgctgatgg gcgcctgcga
    11581 cgccacaatc ctcgcctgga cccgggccgg ctgtccgccg ggcctcgtcg acaaggccga
    11641 catcatcatt gaactcttca tccggggcgc tcaagcacca tgaccaccgc atccactcca
    11701 cggcccttcg cgctctcctc ccgcctgctg ctggcagcgt cgctgctgac cggcgtcgcc
    11761 tgcgacagcg gcgaggagaa gaagcccggc ctcccgccgg ccaaggacgg cgcctccgtc
    11821 ccggcgatcc ccggtccgcc gcccaagtcg gaggtcgggc cggccggcgc cggtgcgagc
    11881 gcgagcgcct cgaccggcgt cgccccggtg accgcccgct acgtcgcgac cgcgctgccc
    11941 aagcacagcg ccgagctcgg cccgcgcatg agcggcaccc tgaccgccgt gctcgtcgag
    12001 gagggcgacc acgtcaagaa gggccagcag ctgttccgcc tcgactcacg caacagccgt
    12061 ctcggcgtcg agcaggccga ggccggcctg cagggcgccg cgatcgcccg cgacaacgcc
    12121 cagcgagagc tcgagcgcca gcgcgtgctc gccgagaagg gcacgatctc cgcggccgtg
    12181 ctcgagcgcg ccgaggcgac cttcaacagc acggtcaacg gcgtcaagca ggccgaggtc
    12241 atgctgtcga tggccaagcg cagcaccagc gactccgcgg tcgtctcgcc gatcgacggc
    12301 gtcgtcgcca agaagctcaa gagcgtcggc gagacggtga cgatgatgcc gccgacgacc
    12361 gtcgtgctga tccaggacca ctcggtgatc gagctgcgca cgcggatccc cgagagcacg
    12421 ctcaagcacg tcaaggaggg cgagctgatc accgcgcact ttaccgcgat cgacgcgacc
    12481 cgcgaggcca gggtggtccg catccagccg accgtcgacc cgatgacgcg cacgatcgag
    12541 atcgtcgccg acgtcgacaa caaggaca
//
LOCUS       DASWNJ010001106         5601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_443529, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001106 DASWNJ010000000
VERSION     DASWNJ010001106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443529"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1683
                     /locus_tag="VGB85_18615"
     CDS             <1..1683
                     /locus_tag="VGB85_18615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136110.1"
                     /translation="DAFYDRLTEIFNDTLLTDRYINGNEALQQLDENTYPRYWYESIE
                     DEEQKNTVIYHANRGVAREALNLVNYIVRNDKPYTEILTADYQMFTPFSAKSYGVEVK
                     FNDPNDPTEIAPGKLPNVPHAGVLTTTMFLSRFPTTATNRNRARARVVYKFFLDTDVL
                     KLGDRPLDPSSIITVNPTMNNASCTVCHELIDPIAATFQNYQAGGNVYKPLDGTEQMT
                     WFPDMRLPGFGDQQLPEGEEKNSEQWLAKQIVADDRFARSAVRIVWKGLIGQDFLSEP
                     FDAAQPDYVEALNAYQVQERVVNSIADKLRSNNYNLKIVFKEIIKTDYFRAKNVTGEL
                     DDSRKYALADLGTAMFLSPEQLNRKIEAVTGYPWAPGPDQQDFLINGNEYRIFYGGID
                     SNSITQRITDPNGIMANISLRMANEMACIVTARDFSQDPSDRLLFPYVEPGFVPEDDN
                     GFAVPAVTDSIRANIQHMYDHVLGESYALTDQEITEAYNLFVTIWKDGKNGVAAGTYG
                     PELAGPCRSTNDYWTGNDLPEERKVYADPDYTVRAWMGVVTYMLSDYKFLHE"
     gene            1695..3011
                     /locus_tag="VGB85_18620"
     CDS             1695..3011
                     /locus_tag="VGB85_18620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY0136111.1"
                     /translation="MDRRKFLSIASLAGLAVASTDAFGSRPDGITKKSSPLFESYRGP
                     LYIMVNASGGWDPTSLCDPKGAANLENPGDAMNRSYLTSAIGETPSGIRYAPLGKDDD
                     PDYFKKFFEEHDKRLLVINGIDFASNGHDTGSRHIWSGRLAEGYPSFAALASAVLGRD
                     LAMSYLSFGGYDETQGQVARTRSGNTQALARIAYPDRMDPGNAESLFHSDNAAKAILD
                     AQNARKERLLAKEQLPRVQRAVSVMYASRTGSNELKKLQEFLPADLDNSNNPLLRQIQ
                     VAVAAYKAGITVAVNLDIGGFDTHGDHDNQHIPRLAQVLEGLDFIVKECNRQSVGDNY
                     VVMAGSDFGRTPGYNDTNGKDHWAVSSVIAFGKGIRGGRAIGETSERHDVLTVNKTTF
                     KADPAGIRIEPRHIHQNLRRLAGIEKDELMNFYPLAIPAEDDLQLF"
     gene            3159..5063
                     /locus_tag="VGB85_18625"
     CDS             3159..5063
                     /locus_tag="VGB85_18625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136112.1"
                     /translation="MSLRRTHPLALLSLYALACGSGCGGDSPAGTEDTGGSTGTGGGD
                     ASSTTDDTPTGEPMDFGPESELVLRLNDKPAAPLSLNMNKAEVAELFGATARDIQLIE
                     VESKTLLTETLEKIKNACGTEWTKDDPNPNHNCAATPLGQTFKGWDNTWKTSPEYSLI
                     RILTMTPANCSVEGTSIEGMSGLAFAIDNDQCEGQNGGDCDFNGMLAESLGIGRTEEF
                     ITTPELVASLQRNLLATHPAIGGDGSKIPVNLEDALKDLSTLTTKLGPMGEHPGILAA
                     DFPSYSEVLTPEFRMFAEASSNLRLLDGVDLSKGKEYMSTIVDLTGPDFDNALEFDFA
                     DPAKFSITGVADDPRVDMRFAVRESAEFVNSCAGADVCKQNVPENQDYVDANFPGSAW
                     AIDRWLLEAIIVTGGREKYKNRVFSKCYEVWNLCAADITIGNPAGWTKFDVTFNLGNP
                     PKDQYVWEMISEVAQVALHTPPAGDIAEGQANVEFTLFDVPIGLNGPEIAESVRPYLQ
                     QQAGKISELLLGDYKKNNGAVDFYYRRGADGLPYLFFVTADDLADGTPADHYKNPGFF
                     SCPEVDAGCKVSQLELGGAGDSTHEKFKLPAGDTTLYLQDDEGKVYRATFEVPEGDAV
                     EIGVRVAAKL"
     gene            complement(5089..>5601)
                     /locus_tag="VGB85_18630"
     CDS             complement(5089..>5601)
                     /locus_tag="VGB85_18630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136113.1"
                     /translation="QPLDTRRIEIPLYIVLDPVKLPNDMTAGGQGVKPVEVTGLHAFV
                     ERDLQKALSQVFASVTVAPPSQPAPSGIFFTGEVLVNTLSTFAGPPAPGTDKPGAYAR
                     MQWSLHIRAYGAPADAFVYQGTAQGTVAITSVRETSPAFQSMFEDALSRLMANMDAQK
                     IYERLSRTGR"
BASE COUNT         1057 a         2082 c         1670 g          792 t
ORIGIN      
        1 gacgccttct acgaccgcct caccgagatc ttcaacgaca ccctgctgac cgaccgctac
       61 atcaacggca acgaggcgct ccagcagctc gacgagaaca cctaccctcg ctactggtac
      121 gagagcatcg aggacgagga gcagaagaac accgtcatct accacgccaa ccgcggcgtc
      181 gcccgcgagg cgctcaacct cgtcaactac atcgtccgca acgacaagcc gtacaccgag
      241 atcctgaccg ccgactacca gatgttcacg ccgttctcgg cgaagagcta cggcgtcgag
      301 gtcaagttca acgacccgaa cgacccgacc gagatcgcgc ccgggaagct gccgaacgtg
      361 ccccacgccg gcgtcctgac gacgacgatg ttcctgagcc ggttcccgac gaccgcgacc
      421 aaccgcaacc gcgcccgcgc ccgcgtcgtc tacaagttct tcctcgacac cgacgtcctc
      481 aagctcggcg accgcccgct cgaccccagc tcgatcatca ccgtcaaccc gacgatgaac
      541 aacgccagct gcacggtctg ccacgagctg atcgacccga tcgcggcgac cttccagaac
      601 taccaggccg gcggcaacgt ctacaagccg ctcgacggca ccgagcagat gacctggttc
      661 cccgacatgc gcctgccggg ctttggcgat cagcagctgc ccgagggcga ggagaagaac
      721 agcgagcagt ggctcgccaa gcagatcgtc gccgacgacc gcttcgcccg ctccgcggtg
      781 cgcatcgtgt ggaagggcct cattggtcag gacttcctga gcgagccctt cgacgccgcc
      841 cagcccgact acgtcgaggc gctcaacgcc taccaggtgc aggagcgggt cgtgaactcg
      901 atcgcggaca agctccgcag caacaactac aacctgaaga tcgtcttcaa ggagatcatc
      961 aagaccgact acttccgggc caagaacgtc acgggcgagc tcgacgacag ccgcaagtac
     1021 gcgctggccg acctcggcac cgcgatgttc ctcagcccgg agcagctcaa ccgcaagatc
     1081 gaggccgtca ccggctaccc gtgggcgccc ggccctgatc agcaggattt cctgatcaac
     1141 ggcaacgagt accgcatctt ctacggcggc atcgactcca actcgatcac gcagcgcatc
     1201 accgacccca acggcatcat ggcgaacatc tcgctgcgga tggcgaacga gatggcctgc
     1261 atcgtcaccg cccgcgactt ctcgcaggac ccctcggacc gcctgctgtt cccctacgtc
     1321 gagcccggct tcgtccccga ggacgacaac ggcttcgccg tccccgcggt caccgactcg
     1381 atccgcgcca acatccagca catgtacgac cacgtgctcg gcgagtccta cgcgctcacc
     1441 gatcaagaga tcaccgaggc ctacaacctc ttcgtcacga tctggaagga cggcaagaac
     1501 ggcgtcgccg ccgggaccta cggccccgag ctcgccggcc cgtgccgctc caccaacgac
     1561 tactggaccg gcaacgacct ccccgaggag cgtaaagtct acgccgaccc cgactacacg
     1621 gtccgcgcct ggatgggcgt cgtcacgtac atgctgtcgg attacaagtt cttgcacgag
     1681 taggaggtgt catcatggac cgtcgcaaat tcctcagcat cgcatccctc gcgggtctcg
     1741 ctgtcgcctc tacggacgcc ttcggcagtc gccccgatgg catcacgaag aagtcgtccc
     1801 ccctgttcga gagctacagg ggcccgctct acatcatggt caatgccagc ggcggctggg
     1861 acccgacctc gctgtgcgac cccaagggcg ccgcgaacct cgagaacccg ggcgacgcga
     1921 tgaaccgctc gtacctgacc tcggcgatcg gcgagacgcc cagcggcatc cgctacgcgc
     1981 cgctcggcaa ggacgacgac cccgactact tcaagaagtt cttcgaggag cacgacaagc
     2041 gcctgctcgt gatcaacggc atcgacttcg cgagcaacgg ccacgacacc ggctcgcgcc
     2101 acatctggtc gggccggctc gccgagggtt acccgagctt cgcggcgctc gcctccgcgg
     2161 tcctcggccg cgacctggcg atgagctacc tcagcttcgg cggctacgac gagacccagg
     2221 gtcaggtcgc gcgcacgcgc agcggcaaca cccaggcgct cgcccgcatc gcctacccgg
     2281 accgcatgga cccgggcaac gcggagtcgc tgttccactc ggacaacgcc gccaaggcga
     2341 tcctcgacgc gcagaacgcc cgcaaggagc gcctgctcgc caaggagcag ctcccgcgcg
     2401 tgcagcgggc cgtcagcgtc atgtacgcct cgcgcaccgg ctccaacgag ctcaagaagc
     2461 tgcaggagtt cctgccggcg gacctcgaca actcgaacaa cccgctgctg cgccagatcc
     2521 aggtcgcggt cgcggcttac aaggccggca tcaccgtcgc cgtcaacctc gacatcggcg
     2581 gcttcgacac ccacggcgac cacgacaacc agcacatccc gcgcctcgcc caggtcctcg
     2641 agggcctcga cttcatcgtc aaagagtgca accgccagag cgtcggcgac aactacgtcg
     2701 tcatggccgg ctccgacttc ggccgcaccc ccggctacaa cgacaccaac ggcaaggacc
     2761 actgggccgt cagcagcgtg atcgcgttcg gcaagggcat ccgcggcggc cgtgcgatcg
     2821 gcgagaccag cgagcgccac gacgtcctca ccgtcaacaa gacgaccttc aaggccgacc
     2881 ccgccggcat ccgcatcgag ccccggcaca tccaccagaa cctgcgccgc ctcgccggca
     2941 tcgaaaagga cgagctgatg aacttctatc cgctcgccat ccccgccgag gacgacctcc
     3001 agctgttttg acctgagtcc gaacccccgg ctttcgggcc cggggggcgc ccacgctcca
     3061 gcgtggacac cggcgcctgg cgcgtccgca gccgcgggcc accaggcgcc gacgtattcg
     3121 tggcacaggg actctggtag ggttcaaagc tcccggccat gtctttacgt cgcacgcacc
     3181 ccctcgccct cctgtccctg tacgccctcg cctgcgggtc cggctgcggc ggggactccc
     3241 cggccgggac cgaggacacg ggcggcagca ccggcaccgg tggcggtgac gcgtcgagca
     3301 cgaccgacga cacgccgacg ggcgagccga tggacttcgg ccccgagagc gagctcgtgc
     3361 tgcgcctcaa cgacaagccg gcggccccgc tgtcgctcaa catgaacaag gccgaggtgg
     3421 ccgagctgtt cggggccacc gcgcgcgaca tccagctgat cgaggtcgag tcgaagaccc
     3481 tgctgaccga gaccctcgag aagatcaaaa atgcctgcgg cacggagtgg accaaggacg
     3541 atccgaaccc gaaccacaac tgcgccgcga ccccgctcgg tcagaccttt aaaggctggg
     3601 acaacacctg gaagaccagc cccgagtact cgctgatccg gatcctgacg atgaccccgg
     3661 ccaactgttc ggtcgagggc acctcgatcg agggcatgtc gggcctcgcc tttgcgatcg
     3721 acaacgacca gtgcgagggg caaaacggcg gcgactgcga cttcaacggc atgctcgccg
     3781 agtcgctcgg catcggtcgc accgaggagt tcatcaccac ccccgagctc gtcgcctcgc
     3841 tgcagcgcaa cttgctcgcc acgcacccgg cgatcggcgg cgacgggtcg aagatccccg
     3901 tcaacctcga ggacgcgctc aaggacctct cgacgctgac gaccaagctc ggcccgatgg
     3961 gcgagcaccc cggcatcctc gccgccgact tcccgagcta cagcgaggtg ctgacgcccg
     4021 agtttcgcat gttcgccgag gcgagcagca acctgcgcct gctcgacggc gtcgacctgt
     4081 ccaagggcaa ggagtacatg tcgacgatcg tcgacctgac cggccccgac ttcgacaacg
     4141 cgctcgagtt cgacttcgcc gaccccgcca agttctcgat caccggcgtc gccgacgacc
     4201 ccagggtcga catgcgcttc gccgtccgcg agtccgccga gttcgtcaac tcgtgcgccg
     4261 gcgccgacgt ctgcaagcag aacgtgcccg agaaccagga ctacgtcgac gcgaacttcc
     4321 cgggatccgc ctgggcgatc gaccgctggc tgctcgaggc gatcatcgtc accggtggcc
     4381 gcgagaagta caaaaatcgc gtctttagca agtgctacga ggtctggaac ctgtgcgcgg
     4441 ccgacatcac gatcggcaac ccggccggct ggaccaagtt cgacgtcacc ttcaacctcg
     4501 gcaacccgcc caaggaccag tacgtctggg agatgatcag cgaggtcgcc caggtcgccc
     4561 tgcacacccc gccggccggc gacatcgccg agggtcaggc caacgtcgag ttcaccctct
     4621 tcgacgtccc gatcggcctc aacggccccg agatagcgga gtccgtgcgc ccctacctgc
     4681 agcagcaggc cggcaagatc tccgagctgc tgctcggcga ctacaagaag aacaacggcg
     4741 ccgtcgactt ctactaccgc cgcggcgccg acggcctgcc ctacctgttc ttcgtcaccg
     4801 ccgacgacct cgccgacggc acccccgccg accactacaa gaatccgggc ttcttcagct
     4861 gccccgaggt cgacgccggc tgcaaggtct cgcagctcga gctcggcggc gccggtgaca
     4921 gcacccacga gaagttcaag ctcccggccg gcgacaccac gctctatctc caggacgacg
     4981 agggcaaggt ctaccgcgcc accttcgagg tcccggaggg cgacgccgtc gagatcggcg
     5041 tccgcgtcgc ggccaagctc tgaccgacca cgaccgtgtg accggcctct agcggccggt
     5101 ccgcgacagc cgctcgtaga tcttctgggc gtccatgttg gccatcagcc ggctcagggc
     5161 gtcctcgaac atgctctgaa acgccggcga ggtctcgcgc accgacgtga tcgccacggt
     5221 cccctgggcg gtgccctggt acacgaacgc gtcggcgggc gccccgtagg cccggatgtg
     5281 caggctccac tgcatgcggg cgtaggcgcc gggcttgtcg gtgccgggcg cgggcgggcc
     5341 ggcgaaggtc gagagcgtgt tcaccagcac ctcgccggtg aagaagatcc ccgagggcgc
     5401 cggctggctc ggcggcgcga cggtgacgct ggcgaacacc tgcgacagcg ccttctggag
     5461 atctcgctcg acgaaggcgt gaagccccgt cacctcgacc ggcttgaccc cctggccgcc
     5521 cgccgtcatg tcgttgggca gcttcaccgg gtcgagcacg atgtagagcg ggatctcgat
     5581 cctgcgcgtg tcgagcggct g
//
LOCUS       DASWNJ010001107         4975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_56284, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001107 DASWNJ010000000
VERSION     DASWNJ010001107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56284"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VGB85_18635"
     CDS             <1..1083
                     /locus_tag="VGB85_18635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEY0136114.1"
                     /translation="VVEYGDIIEGVTMQVRVDEITNHSSMVIQESRDPDARPRFVIKN
                     EDGEVLRVHGKHEARYFLPVGAAFTMTEGTRIQAGDVIAKMPRESTKTKDITGGLPRV
                     VELFEARTPKDHAIISEIDGVVSFGKDKGGKRRIIVAPEHGDPKEYLVPKVRHLLVRE
                     GDRVRAGEKLMDGPANPHDILAVLGEKELASYLLDEIQEVYRLQGVKINDKHIETIIR
                     QMMRRVRVKEAGDTNFLADDHVERQIFQQENNRVASLGGQPATGQALLLGITKASLST
                     ESFISASSFQETTKVLTEAALQGKIDFLRGLKENVIMGRLIPAGTGLSVYERLHMGSD
                     DSSYTDAAAEPVARRTIERDLINLQD"
     gene            1410..1781
                     /gene="rpsL"
                     /locus_tag="VGB85_18640"
     CDS             1410..1781
                     /gene="rpsL"
                     /locus_tag="VGB85_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEY0136115.1"
                     /translation="MPTINQLVRKGRTQQKKKGTAPALRSCPQKRGVCVRVYTCTPKK
                     PNSALRKVARVRLTNGMEVTTYIPGEGHNLQEHSMVLIRGGRVKDLPGVRYHVVRGTL
                     DTTGVNGRRQRRSKYGAKRPK"
     gene            1812..2300
                     /gene="rpsG"
                     /locus_tag="VGB85_18645"
     CDS             1812..2300
                     /gene="rpsG"
                     /locus_tag="VGB85_18645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEY0136116.1"
                     /translation="MSRKGITTRERPSNDIRYTDHPQEMNRNIHKFICGIMYDGKKSI
                     AEKIVFGAFDIISERTKDDPVKVFEKAVANVFPRLEVRSRRVGGSNYQVPVEVRPERG
                     MALAYRWLIGFARSRPEKTMRERLANEMLDAAQNRGGAVKKREDTHKMADANKAFAHY
                     RW"
     gene            2528..4666
                     /gene="fusA"
                     /locus_tag="VGB85_18650"
     CDS             2528..4666
                     /gene="fusA"
                     /locus_tag="VGB85_18650"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY0136117.1"
                     /translation="MQSPENIAAIRDIGIMAHIDAGKTTTSERILFYTGISHRMGEVH
                     DGAAVMDYMPQEQERGITITSAATTCFWKGHRINLIDTPGHVDFTIEVERSLRVLDGA
                     VAVFDAVAGVQPQSETVWRQATRYNVPRVAFINKMDRIGATLDLSVNMMRANLHAHPL
                     VAQLPIGVESAFSGVIDLMTMETIHWPDELGEQVVREPVTEEHELHTEAVLAREALVE
                     AVAEVDDEVMGVFLEAGVGGVPLELLRAGMRRATISNKGVVVLCGSSLKNKGVQPLLD
                     AILDYLPSPLDLPPVLAVRKHDKKEVERLPSADQPLLALAFKVLHDPHRGPLVFFRVY
                     SGALRVKDGIHNCTQDRKERVTKLLQVHANKTQEIDEVTAGNIAAAVGLRFTTTGDTV
                     ISAKDGEQVILPGLEIPEPVIFRAIEPRSTADQAALVEALDRMQREDPSFRVRIDPDS
                     GQTLMGGMGELHLEIIVDRIGREAKVAANVGKPQVAYRETAGQRLTLPIEYDREIGGK
                     RQYARLVLEVVPRERGAGNLFSNLLPEPVARTGKGGPPAVPAKLLPDMITAVREGASD
                     AMSRGPLLGYAVVDVEIRLLDGGYVEGDSTPMSFRAATAMGLMEALEKATPRLLEPVM
                     KVEIEVPEDFTGHVVSDLNGRRGRILGMGPRRGLQVVDSEVPLAEMVGYATALRSSTQ
                     GRASYSMHFSQYAEVPLDLQAAIITRSRGY"
     gene            4812..>4975
                     /locus_tag="VGB85_18655"
     CDS             4812..>4975
                     /locus_tag="VGB85_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0136118.1"
                     /translation="MAKETFVRNKPHVNIGTIGHVDHGKTTLTAAITRVLSLKGWAEF
                     KGYDQIDKAP"
BASE COUNT          934 a         1788 c         1520 g          733 t
ORIGIN      
        1 gtggtcgagt acggcgacat catcgagggc gtgaccatgc aggtccgcgt cgacgagatc
       61 accaaccact cgagcatggt catccaggag agccgcgacc ccgacgcacg cccccgcttc
      121 gtcatcaaga acgaggacgg cgaggtcctg cgggtccacg gcaagcacga ggcgcgctac
      181 ttcctcccgg tcggcgccgc cttcacgatg accgagggca cgcgcatcca ggccggcgac
      241 gtcatcgcca agatgccgcg cgagagcacc aagaccaagg acatcacggg cggtctgccg
      301 cgcgtggtcg agctcttcga ggcgcggacc cccaaggacc acgcgatcat ctcggagatc
      361 gacggcgtcg tgtcctttgg caaggacaag ggcggcaagc gccgcatcat cgtcgcgccc
      421 gagcacggcg acccgaagga gtacctggta cccaaggtcc gccacttgct cgtccgcgag
      481 ggcgaccgcg tccgcgccgg cgagaagctg atggacggcc cggccaaccc gcacgacatc
      541 ctcgcggtgc tcggcgagaa ggagctcgcc tcgtacctgc tggacgagat ccaggaggtc
      601 tatcgcctgc agggcgtgaa gatcaacgac aagcacatcg agacgatcat tcgtcagatg
      661 atgcgccgcg tccgcgtcaa ggaggcaggg gacaccaact tcctcgccga cgaccacgtc
      721 gagcggcaga tcttccagca ggagaacaac cgcgtcgcct cgctcggcgg tcagccggcc
      781 acgggccagg cgctgctgct cggcatcacc aaggcctcgc tgtcgacgga gagcttcatc
      841 agcgcctcgt cgttccagga gaccaccaag gtgctcaccg aggcggccct ccaggggaag
      901 atcgacttcc tccgcggcct caaggagaac gtcatcatgg gccgcctgat ccccgcgggc
      961 accggcctct ccgtctacga gcgcctgcac atgggctcgg acgacagctc ctacaccgac
     1021 gctgcggccg agccggtcgc ccgccgcacc atcgaacgcg acctcatcaa tcttcaggat
     1081 tgaggtccaa agcccgggcg ggagatctcc atcccgcccg gcgctgtctc acgcgcgaac
     1141 tcgccgccga cccttcgcgg tccgcccgat ttccccgcga ttcgtccaaa cgaagcccgg
     1201 cgccctcctg gccgggtttt aggaagtctc ccccgcctcg atcccgcgga tggtccgcct
     1261 ggaacgatgc ttgatctggg ccttgcccac ctatataacc agcgccccac gcccgaagcg
     1321 ggcgaggacg ctcaaagcct ggcgaaacgt cgctcctcca tcgggagcca ccaaacgccg
     1381 gcgatttgcc tcgaaagcct tcactgcgca tgccgacgat caaccagctt gtccgcaagg
     1441 gacgtaccca gcaaaagaag aaggggaccg ccccggccct ccgtagctgc ccgcagaagc
     1501 gcggcgtgtg cgtccgcgtg tacacctgca cgccgaagaa gccgaactcg gcgctccgca
     1561 aggtcgcccg cgtgcgcctc accaacggta tggaggtcac gacctacatc ccgggtgagg
     1621 gtcacaacct tcaggagcac tcgatggtgc tgatccgcgg cggtcgcgtg aaggacctcc
     1681 ccggcgtccg ctaccacgtg gtccgcggca ccctcgacac caccggcgtc aatggccgtc
     1741 gccagcgtcg ctccaagtac ggcgccaagc gcccgaagta gcccgctcgc cgaaagaatt
     1801 aggaagctga tatgtcccgt aaaggcatta ccacccgcga gcggccgagc aacgacatcc
     1861 gctacaccga tcatccccag gagatgaatc ggaacatcca caagttcatc tgcggcatca
     1921 tgtacgacgg caagaagtcg atcgccgaga agatcgtctt cggcgcgttc gacatcatct
     1981 ccgagcgcac caaggacgac cccgtcaagg tgttcgagaa ggccgtcgcc aacgtgttcc
     2041 cccgcctcga ggtccgctcg cgccgcgtcg gcggctcgaa ctaccaggtc cccgtcgagg
     2101 tccgccccga gcgtggcatg gccctggcat accgctggct gatcgggttc gcccgcagcc
     2161 gccccgagaa gaccatgcgc gagcgcctcg ccaacgagat gctcgacgcg gcgcagaacc
     2221 gcggcggcgc cgtgaagaag cgcgaagaca cccacaagat ggccgacgcc aacaaggcgt
     2281 tcgcccacta ccgctggtag tccgaggccg gcccgagccg gccaccagaa ttcccttact
     2341 gacctgaaca accttcgatc ccaccgagcc ctgtacctct ctctttttaa gagaaggagg
     2401 tacctggcac cgtgggaagc ctcccgggcc cctcacccgc cacacccgct ccaaatggag
     2461 acggatggcc ggtggagcgg cccttggctc gttcccacag aactcctctc gtcccgcaac
     2521 tcctcccatg caatcccccg agaacatcgc ggccatccga gacatcggaa tcatggccca
     2581 catcgatgcc gggaagacca cgacgtccga gcggatcctg ttctacacgg gcatctcgca
     2641 tcgcatgggc gaggttcacg acggcgcggc cgtcatggat tacatgccgc aggagcagga
     2701 gcgtggcatc acgatcacgt cggcggcgac cacctgcttc tggaagggtc accgcatcaa
     2761 cctgatcgac acgcccgggc acgtcgattt tacgatcgag gtcgagcgct cgcttcgcgt
     2821 gctcgatggc gcggtcgccg tgttcgacgc ggtcgccggc gtccagccgc agtccgagac
     2881 cgtgtggcgc caggccaccc gctacaacgt cccgcgcgtc gccttcatca acaagatgga
     2941 ccgcatcggc gcgacgctcg acctctcggt caacatgatg cgcgccaacc tgcacgcgca
     3001 tcccctggtc gcccagctgc cgatcggcgt cgagtcggcg tttagcggcg tcatcgacct
     3061 gatgacgatg gagacgatcc actggccgga cgagctcggc gagcaagtcg tccgcgagcc
     3121 ggtcacggaa gagcacgagc tccacaccga ggccgtcctg gcccgtgagg cgctggtcga
     3181 ggccgtggcc gaggtcgacg acgaggtcat gggcgtgttc ctcgaggccg gcgtcggcgg
     3241 cgtcccgctc gagctgctgc gcgcaggcat gcgccgcgcg acgatctcca acaagggcgt
     3301 cgtcgtcctg tgtggctcct cgctcaagaa caagggcgtc cagcccctgc tcgacgcgat
     3361 cctcgactac ctcccgtcgc cgctggatct cccgccggtg ctcgccgtgc gcaagcacga
     3421 caagaaggag gtcgagcgcc tgccctcggc cgaccagccc ctgctggccc tcgccttcaa
     3481 ggtcctgcac gacccgcacc gcggcccgct cgtgttcttc cgcgtgtatt ccggggccct
     3541 tcgggtcaag gacggcatcc acaactgcac gcaggaccgc aaggagcggg tcaccaagct
     3601 gttgcaggtg cacgccaaca agacccagga gatcgacgag gtcaccgccg gcaacatcgc
     3661 cgcggccgtc ggtctgcgct tcacgaccac cggtgacacg gtgatctcgg ccaaggacgg
     3721 cgagcaggtg atcttgcccg gcctggagat ccccgagccc gtgatcttcc gcgcgatcga
     3781 gccccgctcg accgccgacc aggccgcgct cgtcgaggcc ctcgaccgga tgcagcgcga
     3841 ggaccccagc ttccgcgtcc gcatcgaccc cgactccggc cagaccctca tgggcggcat
     3901 gggcgagctg cacctcgaga tcatcgtcga ccgcatcggc cgcgaggcca aggtcgccgc
     3961 caacgtcggc aagccgcagg tcgcctaccg cgagaccgcc ggccagcgcc tgaccctgcc
     4021 gatcgagtac gaccgggaga tcggtggcaa gcgccagtac gcccggctcg tcctcgaggt
     4081 cgtgccccgc gagcgcggcg ccggcaacct ctttagcaac ctcctgccgg agccggtcgc
     4141 tcgcaccggc aagggcggcc cgcccgccgt accggccaag ctgctgccgg acatgatcac
     4201 cgccgtccgc gagggtgcca gtgacgccat gtcccgcggc ccgctcctcg gatacgccgt
     4261 cgtcgacgtg gagatccgcc tcctcgatgg tggttacgtc gagggtgatt caactcccat
     4321 gagcttccgg gccgccacgg ccatgggtct catggaagcc ctcgaaaagg ccaccccgag
     4381 gctcctggag cccgtaatga aggtggagat cgaagtcccc gaggatttca ccggccacgt
     4441 cgtgtccgat ctcaatggcc gccggggaag gatcctcgga atgggacccc gtcgcggcct
     4501 tcaagtggtc gattcggagg ttcccttggc agaaatggtc ggttatgcta ccgccctccg
     4561 ctcaagtaca cagggtcgcg cgagctactc gatgcacttc agccagtacg ccgaggtccc
     4621 cctggacctg caggccgcca tcatcacccg gagccggggc tactgatccc gggacgagac
     4681 ttcacggcct gtaaagtctc gtacgtcagc gaatgatcgg gctttcgcaa agccgcgcaa
     4741 gctggtagat cccgcgacct tcctgcaatc cccccgccaa gacccctgag caagaagacg
     4801 agaagagcgc catggcgaaa gaaacatttg tccgcaacaa gccccacgtg aacatcggaa
     4861 cgatcggaca cgtggatcat ggcaagacca cgctgacggc ggcgatcact cgcgtgctga
     4921 gcctcaaggg ctgggcggag ttcaagggtt acgaccagat cgacaaggct ccgga
//
LOCUS       DASWNJ010001108         2926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_371807, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001108 DASWNJ010000000
VERSION     DASWNJ010001108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371807"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="VGB85_18660"
     CDS             complement(<1..709)
                     /locus_tag="VGB85_18660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136119.1"
                     /translation="MSRRPLLALLSAVLGTACGDSSGGDTGSATAGMTQGETSTGATA
                     STASTASTGPTTSTPTTDPGSSTSPTTTLTTSPTDATTGPITTTDPGGPTGDPTGCVP
                     TTCAAAGAMCGALADDCGGTLDCGACPGDQVCGNGVGPNQCGGPACKPAEAATTNGGG
                     RCADLKEAWTARGINAGYYPYRVVAPVAQYSGDYSGDPAKIFEAGPATKGVILQIIPP
                     GSYIGLSTTGPWYNPAGD"
     gene            933..1658
                     /locus_tag="VGB85_18665"
     CDS             933..1658
                     /locus_tag="VGB85_18665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136120.1"
                     /translation="MWTHWRTHFETNARRPLPGAPADIATIPEAWREPLCASLARFQL
                     GEGGEGRIAREIQRATIAGIDADYRVALALFVKEEGRHARILAGMVRSLGGALLRQNW
                     SERLFVAGRRLLGLRCKLLVLLVAEVVGLGFYTALARRLGPCTIAEQLRQISADEAMH
                     LEFHVDFFRTQTTHAAARALFRASWWLVAGLACAVLLLDHARQLGRLEIRRGEVAREL
                     WRLISEVDRRVLATRPGPVPASA"
     gene            1702..2469
                     /locus_tag="VGB85_18670"
     CDS             1702..2469
                     /locus_tag="VGB85_18670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136121.1"
                     /translation="MSTDLYRVRVLDLDPTQRKVTFRVFLVYYDWSGVPTDPSFFFRI
                     LWDEADARFGGGGPLGEEVDVRHYLDEAWVNENTQRFVERVTRLSLRNDPLPAGKTPP
                     TFVYDGYTDEDSLTQGDYEVIVTDARWIAHLKVGQTWQTTSYETRAVMPKGRATRLMD
                     QAEEEEETAGKKTAKRGAAKKTGGKKTAAKKTASKGATKKTAAKKTASKGAAKKTASK
                     GAAKKTAAKKTASKGAAKKTAAKKTAAKKTAKVGASA"
     gene            2616..>2926
                     /locus_tag="VGB85_18675"
     CDS             2616..>2926
                     /locus_tag="VGB85_18675"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0136122.1"
                     /translation="MLRSLQLKRLLSFGPDAGPVELGPLNVLIGPNGSGKSNFLEAIG
                     LLHAAPGDLVRPVREGGGVGAWLWQGEAGATAEIEAVLEHPTSPGGGLRHRIAFAEVG
                     QR"
BASE COUNT          432 a          943 c         1155 g          396 t
ORIGIN      
        1 agtcgccggc ggggttgtac caggggcccg tggtcgacag gccgatgtag ctgccgggcg
       61 gaatgatctg gaggatgacg cccttggtgg ccgggccggc ctcgaagatc ttggccgggt
      121 cgccgctgta gtcgcccgag tactgggcga ccggcgcgac gacgcggtac gggtagtacc
      181 cggcgttgat cccccgggcg gtccacgcct ccttgaggtc ggcgcagcgg cccccaccgt
      241 tggtggtcgc ggcctcggcc ggcttgcacg ccggaccacc acactggttg ggcccgacgc
      301 cgttgccgca gacctggtct ccgggacagg cgccgcagtc gagcgtgcca ccgcagtcgt
      361 cggcgagcgc accacacatc gcgcccgcgg cggcgcaggt ggtcggcacg cagccggtcg
      421 ggtcgccggt cggaccgccc gggtcggtgg tcgtgatcgg gccagtggtc gcgtcggtcg
      481 gcgaagtggt gagggtggtg gtcggcgagg tcgagctgcc cgggtcggtc gtgggggtgc
      541 tcgtggtcgg gccagtcgag gcggtcgagg cggtcgaggc ggtcgcgccg gtcgaggtct
      601 cgccctgcgt catgccggcg gtcgcggacc cggtgtcgcc gccggagctg tcgccgcacg
      661 cggtgccgag gacggcggac agtagggcga gcagcgggcg tcgggacatg cggacctcgg
      721 gtgcgagcgt cctgtatgcc aaacctgcgg cggtcgtggc aaacggcgca ggcgcggacc
      781 gggcagaaaa cggatcgggg tagcggcggc ggcgcggcgg cagcggccgc gaggagggcc
      841 ggcgcgaccg ttcacgggcc gcttgcgacc gttcaccggg aacgagggca ggggggccgc
      901 ctgcggcgcg tagaagcccc ggagacgcga acatgtggac tcactggcgc acgcacttcg
      961 agaccaacgc ccgccgcccg ctgccggggg cgccggccga catcgcgacg atccccgagg
     1021 cctggcgcga gcccttgtgc gcctcgctgg cccgatttca gctcggcgag ggcggcgagg
     1081 gccggatcgc ccgcgagatc cagcgggcga cgatcgccgg catcgacgcc gactaccgcg
     1141 tcgcgctggc gctgttcgtc aaggaggagg gccggcacgc gcggatcctg gccggcatgg
     1201 tgcggtcgct cggaggggcg ctgctgcgcc agaattggag cgagcggctg ttcgtcgccg
     1261 gccggcgcct gctcgggctg cgttgcaagt tgctggtgct gctggtcgcg gaggtcgtcg
     1321 gcctcgggtt ctacacggcg ctggcgcggc gcctcgggcc gtgcacgatc gcggagcaac
     1381 tgcgtcagat ctccgcggac gaggcgatgc acctcgaatt ccacgtcgac ttctttcgca
     1441 cccagacgac ccatgcggcc gcgcgggcgc tgtttcgggc ctcgtggtgg ctcgtcgccg
     1501 ggctcgcctg cgcagttttg ttgctggatc acgctcgcca gctcggtcgg ctcgagatcc
     1561 gccgtggcga ggtggcccgt gaactgtggc ggctgatctc cgaggtcgac cggcgcgtgc
     1621 tcgcgaccag gccggggcct gtgccggcct ccgcttgacc ggacgccggt gatcccggac
     1681 gatcaccgct ggcgtccgac catgtccacc gacctctacc gcgtccgtgt cctcgacctc
     1741 gaccccaccc agcgcaaggt caccttccgg gtgttcctgg tctactacga ctggtccggc
     1801 gtaccgaccg acccgtcgtt cttctttcgc atcctgtggg acgaggccga cgcccgcttc
     1861 ggcggcggcg gaccgctcgg cgaggaggtc gacgtgcgcc actacctcga cgaggcgtgg
     1921 gtcaacgaga acacgcagcg cttcgtcgag cgcgtgaccc gactgtcatt gcgcaacgac
     1981 ccgctgcccg caggcaagac gccgcccacg ttcgtatacg acggctatac cgacgaggac
     2041 agcctgacgc agggcgacta cgaggtgatc gtgaccgacg cgcggtggat cgcgcatctc
     2101 aaggtcggac agacctggca aacgacgtca tacgagacgc gagccgtgat gccaaaaggg
     2161 cgagcgacgc ggctgatgga ccaggcggaa gaggaagagg agacggccgg gaagaagacg
     2221 gcgaagcggg gagcggcgaa gaagaccggc gggaagaaga cggcggcgaa gaagacggcg
     2281 agcaagggcg cgacgaagaa gacggccgcg aagaagacgg cgagcaaggg ggcggcgaag
     2341 aagacggcga gcaagggcgc ggcgaaaaag acggcggcga agaagacggc gagcaagggc
     2401 gcggcgaaga agacagccgc gaagaagacg gcggcgaaga agaccgcgaa ggtgggcgcg
     2461 agcgcatagc tgcggcgcgt ggtcctgggc cgtgacgcgt gccgcgttca cggctcgacc
     2521 cgcagggggg ccgctgcgcg tggctcgcgt taaggcacgc ggttgtgtgg gtttgcttga
     2581 cgggcccgcg gcgatgctgg acgctctgcg cgtccatgct ccgcagcctc cagctgaagc
     2641 gactgctctc gttcggcccc gacgccgggc cggtcgagct cgggccgctc aatgtgttga
     2701 tcgggcccaa cggcagcggc aagtcgaact tcctcgaggc gatcgggctg cttcacgccg
     2761 cgccggggga tctggtgcgg cccgtacgcg agggcggcgg ggtcggggcg tggctgtggc
     2821 agggcgaggc cggggccacg gcggagatcg aggcggtgct cgagcacccg acgagcccgg
     2881 gcggcggctt gcggcatcgc atcgccttcg ccgaggtcgg gcagcg
//
LOCUS       DASWNJ010001109         4326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_197577, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001109 DASWNJ010000000
VERSION     DASWNJ010001109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197577"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(532..1869)
                     /locus_tag="VGB85_18680"
     CDS             complement(532..1869)
                     /locus_tag="VGB85_18680"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY0136123.1"
                     /translation="MPRIDENRRALLVGTAVVGFSILGLVGLVLSGSKTLWNEQVSVT
                     ADFRQVSGLKPGSPVQLEGIEIGLVKDRQFVEIEYPCNPLTEDRGRFEHGRTDDCDRT
                     LFCAPEGKCAELEAYSFNKDFYRTCEVDAQCQESEICITPEFRRRYRRVSWTGNTGLC
                     DRYITDHKRIRVFLDIYADRLPQLRDDSRATVSQNGVLGDQLIAISAGRGKPIEAGGQ
                     IQTIPAMIEELDNVKDRADGLFDKVEATIGGVAELAKAMGDEKTVKNVQAALANTNEV
                     TRRTAEGQGTFGMLLNDETYAKDFGANLRGVRETTASIDRGLGQARRGLNTFEHDLEP
                     AVKTGRDAMEKISKTLVERKDPNSKATAALLLNPEGKLTQDVEDSLAATRRLVEGVNA
                     GEGTAGRLLKDPKVYDDLVGFFQDLGKRTSIRFLVRWARARDTRDPRIPRDVD"
     gene            complement(1884..2738)
                     /locus_tag="VGB85_18685"
     CDS             complement(1884..2738)
                     /locus_tag="VGB85_18685"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0136124.1"
                     /translation="MESTRTQYLSTANAARLLGVGPTSVKRWADLGLLRCVRTAGNHR
                     RFTRTEVERFARAVQAGQVVLDPSARPEPLPPPPPATPEEVAWTDRLLALGPFALHGA
                     LLAERQKLGSWWKVADVIGGALAELGNRWECGKISIIDEHLASERLARGVARCAESLT
                     SPANAPIALLAAAAGDEHTLGLALAELVFREAGYEVGWIGRYTPGDHLAAHLRSHPAA
                     IVALSASGCSQDAQALRTATLPVAHACEEVGARLFLGGGGCWPTDLPASTRVYAFAEL
                     HAALVDAA"
     gene            2851..3780
                     /locus_tag="VGB85_18690"
     CDS             2851..3780
                     /locus_tag="VGB85_18690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEY0136125.1"
                     /translation="MRIFITGATGFVGKALVPRLLRDGHSLRAWARSPARAAEQLGPQ
                     VDLVDAAGGAPAILTAIADADAIINLAGEPVIGKRWSERQREALWASRVGLTDMIVDA
                     IVAAPPRPRVLVSTSAVGYYGDTGDREVVEDSPPATDFLAALTVGWEAAAQRAEAHDT
                     RVCIVRVGLVLGREGGVLAKMLPVFRLGLGGPLGSGAQWFPWVHIDDLVELYTQALTD
                     AALRGPVNGVAPGIVTGRDFTSALGRAVHRPTIFRVPAFALRLALGESATAVLAGQRA
                     LPRRTQELGFRFKYSELDAALGDLTATFRPPVA"
     gene            3849..4274
                     /locus_tag="VGB85_18695"
     CDS             3849..4274
                     /locus_tag="VGB85_18695"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY0136126.1"
                     /translation="MTGPQIQPATHLGIDPTLCGEVVTLAPGRAEARLTTLASMAVDA
                     SGLVHGGFVFGLADHAAMLAVNDPNVVLGAASTRFLKPVRAGDVLLARATCTTESGRR
                     RIVAVEVVRGDEPVMTGELTCFVLDRHVLADMSPPPERP"
BASE COUNT          584 a         1512 c         1594 g          636 t
ORIGIN      
        1 tgtcatggct tcgggcgcga cgtggtgctt gcttgcatgc cagatcgcgt gtgcgagcgc
       61 tggcattggc ttcgggtggc cacgcgctac acggcgacag cgcccggcca cgcgccacct
      121 cgtgcacgcg acaagcgggg cgccgacgat gtccgcccgg ggcgcagggg ccgacgagcg
      181 ggggaagcag cgggaggacc caggggtcac gcgcagacgc cccgtcaact tccggacacg
      241 cgatgaaggg tcggtgacag gactctggct atcccgacag atggggcggc gagcatgtcc
      301 cctgggagga cccgcgtcgc agggacccgc agcccgtcgg cccccgcgcc ccggcccagg
      361 aagcctcgcc ccgcggtctc cgagcacgcc cggcccacgc gagcccgggt ctccgagcac
      421 gcccggccca cgcgaggcca cggtctccga gcacgcccga cccgaacacg ctcgccccga
      481 acacgctcgc cccgaacacg cccggcccga acacgccaat caacgaacga ctcagtcgac
      541 atcccgcggg atccgcggat cccgcgtatc ccgagcacga gcccagcgga cgaggaagcg
      601 gatcgaggtg cgcttgccga ggtcctggaa gaagccgacg aggtcgtcgt agaccttggg
      661 gtccttgagg aggcggccgg ccgtgccttc gccggcgttg acgccctcga cgaggcggcg
      721 ggtcgcggcg agcgagtcct cgacgtcctg ggtcagcttg ccctccgggt tgagcaggag
      781 cgccgcggtg gccttgctgt tggggtcctt gcgctcgacg agggtcttcg agatcttctc
      841 catcgcgtcg cggccggtct tcaccgcggg ctcgaggtcg tgttcgaagg tgttgaggcc
      901 gcggcgggcc tggccgaggc cgcggtcgat gctggcggtg gtctcacgga cgccgcgcag
      961 gttggcgccg aagtccttgg cgtaggtctc gtcgttgagg agcatgccga aggtgccctg
     1021 gccctcggcg gtgcggcggg tgacctcgtt ggtgttggcg agggcggcct ggacgttctt
     1081 gacggtcttc tcgtcgccca tcgccttggc gagctcggcg acgccgccga tcgtcgcctc
     1141 gaccttgtcg aacaggccgt cggcgcggtc cttgacgttg tcgagctcct cgatcatcgc
     1201 ggggatcgtc tggatctggc cgccggcctc gatcggcttg ccgcggccgg ccgagatcgc
     1261 gatcagctga tcgccgagca cgccgttttg cgagaccgtc gcgcggctgt cgtcgcgcag
     1321 ctgcggcagg cgatcggcgt agatgtcgag gaacacccgg atccgcttgt gatcggtgat
     1381 gtagcggtca caaaggccgg tgttgccggt ccagctgacg cggcggtagc ggcggcgaaa
     1441 ctcgggggtg atgcagatct cgctctcctg gcactgcgcg tcgacctcgc aggtgcggta
     1501 gaagtccttg ttgaacgagt aggcctcgag ctcggcgcac ttgccctcgg gcgcgcagaa
     1561 cagggtgcga tcgcagtcgt cggtgcggcc gtgctcgaag cggccgcggt cctccgtcag
     1621 cgggttgcag gggtactcga tctcgacgaa ttggcggtcc ttgacgaggc cgatctcgat
     1681 gccctcgagc tgcaccgggc tgccgggttt gaggccggag acctggcgaa agtcggcggt
     1741 gaccgacacc tgttcgttcc acagcgtctt gctgccgctg aggaccaggc cgaccagccc
     1801 gaggatgcta aacccgacca cggcggtgcc gacgagcagg gcgcggcggt tttcgtcgat
     1861 gcggggcata cccgagtgta gcctcaggcg gcgtcgacga gcgcggcgtg cagctcggcg
     1921 aaggcgtaga cgcgggtcga ggccggcaga tcggtcggcc agcagccccc accgcccagg
     1981 aacaggcgcg cgccgacttc ttcgcacgcg tgcgcgaccg gcagcgtggc ggtccgcagc
     2041 gcctgcgcat cctgcgagca gcccgaggcc gacagcgcga cgatcgccgc cgggtggctg
     2101 cgcaggtggg ccgcgaggtg atcaccgggc gtgtagcggc cgatccagcc gacctcgtag
     2161 ccggcctcgc ggaacaccag ctctgccagc gcgaggccga gcgtgtgctc gtcgccggcc
     2221 gccgccgcga gcagggcgat cggcgcgttc gcgggcgagg tcagggactc cgcgcagcgg
     2281 gcgacgccgc gggcgagccg ctccgacgcc aggtgctcgt cgatgatgct gatcttgccg
     2341 cactcccagc ggttgccgag ctcggccagc gcgccgccga tgacgtcggc gaccttccac
     2401 cagctgccga gcttctgccg ctcggccagc agcgcgccgt gcagcgcgaa cggcccgagc
     2461 gcgagcaggc gatcggtcca cgcgacctcc tcgggggtcg ccggcggtgg gggtggcaac
     2521 ggctcgggcc gcgccgacgg gtcgagcacg acctgtccgg cctgcacggc ccgggcgaag
     2581 cgctcgacct cggtgcgcgt gaaccgacgg tggttgccgg ccgtccgcac gcagcgaagc
     2641 aggccgaggt cggcccaacg cttgaccgac gtcggtccga cgccgagcaa acgggccgcg
     2701 ttggcggtgg agagatactg cgttcgcgtg gattccatgc gcgggcgggg tgtcgaacca
     2761 gggatgtggt ggacacgtcg aaacggccgg gatccttgat catccgacca aagcgactat
     2821 aaacctggcc aggcgagagg gcaacgcagc atgcggatct tcatcacagg cgcgaccggt
     2881 ttcgtcggca aggcgctcgt tccccggctc ttgcgcgacg ggcacagcct gcgtgcgtgg
     2941 gcccgatcac cggcgcgtgc ggcggagcag ctcggtccgc aggtcgacct ggtcgatgcg
     3001 gcgggcggcg caccggcgat attgaccgcg atcgcggacg ccgacgcgat catcaatctg
     3061 gcgggcgagc ccgtgatcgg caagcggtgg tcggagcgcc aacgtgaggc gttgtgggcg
     3121 agtcgggtcg gcctgaccga catgatcgtc gacgcgatcg tcgccgcgcc gccgcgcccg
     3181 cgcgtgctcg tctcgacctc cgccgtcggc tactacggcg acaccggcga ccgcgaggtc
     3241 gtcgaggaca gcccgccggc cacggacttc ctcgccgcgc tgacggtcgg gtgggaggcc
     3301 gcggcgcagc gggccgaggc ccacgacacg cgcgtgtgca tcgtccgcgt cggcctcgtg
     3361 ctcggccgcg agggcggcgt gctggcgaag atgctgccgg tgtttcgcct cggtctcggc
     3421 gggccgctcg gcagcggcgc gcagtggttc ccgtgggtcc acatcgacga tctcgtcgag
     3481 ctctacaccc aggccctgac cgacgccgcg ctgcgcgggc cggtcaacgg cgtcgcgccg
     3541 ggcatcgtca ccggtcgcga tttcaccagc gcgctcggcc gcgcggtgca tcgcccgacg
     3601 atcttccgcg tcccggcgtt cgccctgcgc ctcgcgctcg gcgagtcggc gaccgccgtg
     3661 ctcgctggcc agcgggcgct gccccgtcgc acccaggagc tcggctttcg cttcaagtac
     3721 tccgagctcg acgcggccct cggcgacctg acggcgactt tccggccgcc cgtggcctga
     3781 tccgcctccg gccgcccgcg gcagtgggcg gtcgggctcc gcgccgtgtg gtataagcgc
     3841 cgcctcgtat gacgggcccg cagatccagc cggcgacgca cctcggcatc gatcccaccc
     3901 tgtgcggcga ggtcgtcacg ctcgcgcccg ggcgcgccga ggcgcggctg acgacgctgg
     3961 cgagcatggc cgtcgacgca agcggcctgg tccacggcgg cttcgtgttc ggcctcgccg
     4021 atcacgcggc gatgctcgcg gtcaacgacc ccaacgtcgt gctcggggcc gcgagcacgc
     4081 gcttcctcaa gcccgtgcgc gccggcgacg tgctgctggc ccgcgccacc tgcacgaccg
     4141 agtcggggcg caggcggatc gtcgcggtcg aggtcgtgcg cggcgacgag ccggtgatga
     4201 ctggcgagct gacctgcttc gtgctcgatc gacatgtcct cgcggacatg tcaccacccc
     4261 ccgagagacc ctgagccaca ttggaggccc gaatggacgg cggcgcgctg atcgcagagg
     4321 tgttgc
//
LOCUS       DASWNJ010001110         1774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_656562, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001110 DASWNJ010000000
VERSION     DASWNJ010001110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656562"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VGB85_18700"
     CDS             complement(<1..196)
                     /locus_tag="VGB85_18700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136127.1"
                     /translation="MPDSKFEDIATIPGICEVSWYAPTGGAGAPCLLIEVPHGATRSA
                     DFHDLRARLTGELPDQLDHFF"
     gene            complement(285..929)
                     /locus_tag="VGB85_18705"
     CDS             complement(285..929)
                     /locus_tag="VGB85_18705"
                     /inference="COORDINATES: protein motif:HMM:NF012523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatA-like O-acetyltransferase"
                     /protein_id="HEY0136128.1"
                     /translation="MRPGSPTSNFRVLGDYPRRAHLEFYRRYPNPFYSVTFELDATRL
                     RAAIADAGVSTYAAFCWAFHRALLDVEAFRVRLHGDDVVLYDELRLGLTVPAPRRTFS
                     FTDLDWHPDAGVFFPAAASVMARASASSELVGGEAPDFAYYTALPRLPFTGFTHVALP
                     DPLAGQPEIAFGRFAERDARVRLPVGLQVNHVYVDGADLGELYEAAQDSFDRAF"
     gene            1005..>1774
                     /locus_tag="VGB85_18710"
     CDS             1005..>1774
                     /locus_tag="VGB85_18710"
                     /inference="COORDINATES: protein motif:HMM:NF041328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_6577-like cysteine-rich protein"
                     /protein_id="HEY0136129.1"
                     /translation="MSRRLAPVLSCVLACNAGGAVEETGSSGSTGESTGGATDMDSTA
                     STPTTGSGCAAPLGACEGVCVDLDHDPEHCGDCGVVCEVGLTCVEGSCEVACGSGGLV
                     CGEQCVAPDSDPAHCGGCDRPCGEGVACIDGQCAPVCGPDETLCGEQCVALARDEAHC
                     GGCDLACAGDQPCVFGHCVDVAVHHLLIGGQSLSVGYGSQVLSVEQPFDNLSFNTGVR
                     AGAEGLTSFVPLVEAMQGNLGETIASGLANHVAEQQPIV"
BASE COUNT          259 a          565 c          718 g          232 t
ORIGIN      
        1 agaagaagtg gtcgagctga tcgggcaact cgccggtgag gcgcgcgcgc aggtcgtgga
       61 agtcggccga gcgggtcgcg ccgtgcggga cctcgatcag caggcagggc gccccggcgc
      121 cgccggtggg cgcgtaccag ctcacctcgc agatgccggg gatcgtggcg atgtcctcga
      181 acttggagtc gggcacccgg gcaggatgcc ccagcggcgc ggtcgacgtc gaccgatcgc
      241 gcctcgcact tcgcgtgcgc caggtcgctg cgctgcgcgg cgcctcagaa cgcccggtcg
      301 aaggagtcct gggcggcctc gtagagctcg ccgaggtcgg ccccgtcgac gtacacgtga
      361 ttgacctgca gtccgaccgg caggcgaacg cgggcgtctc gctcggcgaa cctgccaaag
      421 gcgatctcgg gctggccggc gagcgggtcg ggcagggcga cgtgggtgaa gccggtgaag
      481 ggcaggcgcg gcagcgcggt gtagtaggcg aagtcgggcg cctcaccgcc gacgagctcg
      541 ctgctcgcgg aggcgcgcgc catcaccgag gcggcggccg ggaagaacac gcccgcgtcg
      601 gggtgccagt cgaggtcggt gaagctgaag gtgcgccgcg gcgcgggtac ggtgaggccg
      661 aggcgcagct cgtcgtacag cacgacgtcg tcgccgtgca ggcgcacgcg aaacgcctcg
      721 acgtcgagca gcgcgcggtg aaaggcccag caaaacgccg cgtaggtcga gacgccggcg
      781 tcggcgatcg cggcgcgcag gcgcgtcgcg tcgagctcga aggtgaccga gtagaagggg
      841 ttcgggtagc ggcgatagaa ctcgaggtgg gcgcggcgcg gatagtcgcc gagcacgcga
      901 aagttcgagg tcggcgagcc cgggcgcatg cgcgccatcg taggcgcccg gggtggcgac
      961 tggcaacttg cggcgcggcg ctcgcagacg gataaaccca ggagatgtcg cgccgcctcg
     1021 ccccggtcct gtcgtgtgtg ctcgcctgca acgccggcgg ggcggtcgag gagaccggga
     1081 gcagcggcag caccggcgag agcacgggtg gggcgacgga catggacagc accgcgagca
     1141 cgccgacgac cgggtccggc tgcgccgcgc cgctcggcgc gtgcgagggc gtctgcgtcg
     1201 acctcgatca cgacccggag cactgcggcg actgtggggt cgtctgcgag gtcgggctca
     1261 cctgcgtgga gggcagctgc gaggtcgcgt gcgggagcgg tggtctggtg tgcggcgagc
     1321 agtgcgtcgc tccggacagc gacccggcgc actgcggcgg gtgtgatcgg ccgtgcggcg
     1381 agggggtcgc gtgcatcgat gggcagtgcg cgccggtgtg tggacccgac gagacgctgt
     1441 gcggcgagca gtgcgtcgcg ctcgcccgcg acgaggcgca ttgcggcggc tgcgacctcg
     1501 cctgcgcggg cgaccagccg tgcgtgttcg gccactgcgt cgacgtcgcg gtccatcacc
     1561 tgctgatcgg cggccagagc ctgtcggtcg gctacggctc gcaggtgctc agcgtcgagc
     1621 aaccattcga taacctgtcc ttcaatacag gtgtgcgcgc cggggccgag gggctgacga
     1681 gctttgtccc gctcgtcgag gcgatgcagg gcaacctcgg cgagacgatc gcgtcggggc
     1741 tggcgaatca cgtcgccgag cagcagccga tcgt
//
LOCUS       DASWNJ010001111         1587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_20506, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001111 DASWNJ010000000
VERSION     DASWNJ010001111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20506"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VGB85_18715"
     CDS             <1..606
                     /locus_tag="VGB85_18715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136130.1"
                     /translation="LAQERRLRPARLIHPMTTPAPSAADIRAALEDLRTADRILLSVQ
                     GTTQFLKRQQFLDTLGIATGDGYFGGETVRDNNHANEELMAAQQAMTDATRRLGLANI
                     EPETLVVWGRLDEIFDGAWSDFLALQQARANQEVAGRVRGRVRALFARACELHPEVAA
                     GVAPLADEAIEDDDLRPARDWQKLGMYVALAIAAVAAVAML"
     gene            643..1065
                     /locus_tag="VGB85_18720"
     CDS             643..1065
                     /locus_tag="VGB85_18720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136131.1"
                     /translation="MLMATLVWLLQAPTLPAAVPFTPSVQEWSLGGAFKLKQEKFLVW
                     APAYDILRGWHVELRPTWIVELTPTHPRLSRLSLAVESNIAGAAGGIGMVRPRLQYKV
                     GKRQTRVGVELPIATLVNTASGFGLRTFRPFAFISGRF"
     gene            complement(1075..>1587)
                     /locus_tag="VGB85_18725"
     CDS             complement(1075..>1587)
                     /locus_tag="VGB85_18725"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0136132.1"
                     /translation="YMLGIQRDVTELMGARREIAVAREEIDAQRNQIDALESARREIE
                     AQKATIQALSTPVIEVWDGIITLPLLGHFNAERASAMTSQLLDAVVRTRARYAILDLT
                     GIAQIDTNTGDHILRIVSAVGLLGATGVLVGIQAEIAQMLVGLGVELGRVRVYQNLRQ
                     ALKACMSPGS"
BASE COUNT          229 a          558 c          548 g          252 t
ORIGIN      
        1 ctcgctcaag agcgtcgcct gcgacccgcc cggctgatcc atcccatgac cacgcctgca
       61 ccctccgcgg ctgacatccg cgccgcgctc gaggacctgc gcaccgccga ccgcatcctg
      121 ctcagcgtgc aggggaccac ccagttcctg aagcgccagc aattcctcga caccctcggg
      181 atcgccaccg gcgatggtta cttcggcggc gagaccgtcc gcgacaacaa ccatgccaac
      241 gaggagctga tggccgccca gcaggcgatg accgacgcga ctcgtcgcct cggcctggcc
      301 aacatcgagc ccgagacgct ggtggtctgg ggccgcctcg acgagatctt cgatggtgcg
      361 tggtccgatt tcctcgcctt gcagcaggcc cgcgccaatc aggaggtcgc cgggcgcgta
      421 cgcggccggg tgcgcgcgct gttcgcccgc gcgtgcgaat tgcaccccga ggtcgccgcc
      481 ggcgtcgcgc cgctcgccga cgaagcgatc gaagacgatg acctgcggcc ggcgcgggac
      541 tggcaaaagc tcggcatgta cgtcgcgctg gcgatcgcag cggtcgcagc agtcgccatg
      601 ctttgagttg tcagcgccgg gccgccgcgc tatcgtacga cgatgctgat ggcgacgctc
      661 gtgtggctgc tacaggcacc gacactgccc gccgccgtgc cgttcacgcc gtcggtgcag
      721 gagtggagcc tcggcggggc gttcaagctc aagcaggaga agttcctggt gtgggcgccc
      781 gcgtacgaca tcctgcgcgg ctggcatgtc gagctgcggc cgacgtggat cgtcgagctg
      841 acgccgacgc acccgcggct gtcgcggctc tcgctcgcgg tcgagtccaa catcgccggt
      901 gctgcgggcg gcatcgggat ggtccgcccg cgcctgcaat acaaggtcgg caagcgtcag
      961 acccgcgtcg gcgtcgagct gccgatcgcc acgctggtca acacggcctc gggcttcggc
     1021 ctgcggacct ttcgcccgtt cgcgttcatc tccgggcgct tctgaggcgg cgcgtcacga
     1081 gcccggggac atgcaagcct tgagggcctg gcgcaggttc tgatacacgc gcacgcgtcc
     1141 gagctcgacg ccgaggccga cgagcatctg ggcgatctcc gcctggatgc cgacgagcac
     1201 cccggtcgcg ccgagcagcc cgaccgcgga gacgatgcgc aggatgtggt cgccggtgtt
     1261 ggtgtcgatc tgggcgatgc cggtgaggtc gaggatcgcg tagcgggccc gcgtgcgcac
     1321 gaccgcgtcg agcagctggg aggtcatcgc gctcgcgcgc tcggcgttga agtgaccgag
     1381 cagcggcaac gtgatgatgc cgtcccacac ctcgatcacc ggggtcgaga gcgcctggat
     1441 cgtcgccttt tgcgcctcga tctcccgcct cgcggactcg agcgcgtcga tctggttgcg
     1501 ctgggcgtcg atctcttcgc gcgcgacggc gatctcgcgg cgcgcgccca tcagctcggt
     1561 gacgtcgcgc tggatgccga gcatata
//
LOCUS       DASWNJ010001112         1843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_512775, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001112 DASWNJ010000000
VERSION     DASWNJ010001112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512775"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1002)
                     /locus_tag="VGB85_18730"
     CDS             complement(166..1002)
                     /locus_tag="VGB85_18730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136133.1"
                     /translation="MCTTLAGQRRAAALVLAMLAACTVPGPSDMPPKTDPAAVPSDMS
                     PQTAPAPACDARDTLGDTSWLPADLRLAVILDLDSPDLPAAIVRLQDGVREGRGLPVV
                     ASRGLGLLGLLLDILRPQLRRAGLDPRELALLHDREGAVVWVLRARCDLEALQARLAA
                     SWSLQVRTLTGGSVAEGRRDGADATGFAFDVAFLAEDRLALTPPGSAPALRRWLSGPP
                     AAPLAGPAAPPPAEVLDEIPAAPIRGVLAGRSLQAPGASTVPLVRTLRATADALEIDG
                     QR"
     gene            complement(1116..>1843)
                     /locus_tag="VGB85_18735"
     CDS             complement(1116..>1843)
                     /locus_tag="VGB85_18735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136134.1"
                     /translation="AALMEGVGPAPSTWLRAEALVSLPPIARGPYLVRGLSRPRAEVE
                     AWLTAGGLARDMMEGMVTYSPLPGEPDAAAAPEALPVHHAPAAAFPWRIVFLEDDVIG
                     CYSLREIGGGLGPLTAARDLPVSELETRLTQEFAEDPEMLLDLVASGPTLSLDISDDV
                     GAVRLGMRRWERSGLDGELILQPLGDIDGATKELEARRPRGETDAVRELYDRIAFTPE
                     PPVVRGRLQMSEADLRLLAREGG"
BASE COUNT          247 a          687 c          699 g          210 t
ORIGIN      
        1 tcggcgacct tgttgaagaa gaagcgcgcg accggctcgt aatagtgatc gaacagcgcg
       61 tgcccggcgg cccggtctcc gcggatccag gcctcgacga gcgcgcggtc gcgggcggcg
      121 tccatgccag cgatggtcgg cgctgcgccg gtcacggtca acgcttcacc gctgaccgtc
      181 gatctcgagc gcgtccgcgg tcgcccgcag ggtccgcacc agcggcacgg tgctcgcgcc
      241 cggggcctgc aggctccggc ccgccagcac gccgcggatc ggggcggccg ggatctcatc
      301 gagcacctcg gccgggggcg gcgcggccgg gcccgccagc ggggcggccg gcggacccga
      361 gagccagcgg cgcagcgccg gcgccgagcc gggcggcgtc agggccagcc ggtcctcggc
      421 gaggaaggcg acgtcgaagg cgaagccggt cgcgtcggcc ccgtcgcgcc ggccctccgc
      481 gaccgatccg cccgtgagcg tgcggacctg caacgaccag ctcgcggcca gccgcgcctg
      541 cagggcctcg aggtcgcagc gggcccgcag gacccacacg acggccccct cgcggtcgtg
      601 cagcagcgcc agctcgcgcg ggtcgagccc ggcgcggcgc agctgcgggc gcaagatgtc
      661 gagcagtaaa ccgagcagcc cgagcccgcg gctggcgacc accggcaggc cccgaccctc
      721 ccgcacgccg tcctgcaggc gcacgatcgc ggccggcagg tcggggctgt cgaggtcgag
      781 gatcacggcg aggcgcaggt ccgcgggcaa ccacgaggtg tcgccgagcg tgtcgcgggc
      841 gtcgcacgcg ggcgctggag ctgtctgtgg ggacatgtcc gacgggacag ccgccgggtc
      901 tgtcttcggg ggcatgtccg acgggccagg cacggtgcag gccgcgagca tcgccaggac
      961 cagcgccgcg gccctcctct gccccgccag cgtcgtgcac atcgacgagt ccattcgctt
     1021 cgcatcgatg gaccggcgcg cgcgatcgat cgaccggagt ccgggcggcc tcgtgggccg
     1081 acctgctcgc ctggacacgg cccgatcgct ggcgctcatc caccctcgcg cgcgagcagg
     1141 cgcaggtcgg cctccgacat ctgcaggcgg ccgcgcacga ccggcggctc gggcgtgaag
     1201 gcgatgcgat cgtagagctc gcgcaccgcg tcggtctcgc ccctcggcct gcgcgcctcg
     1261 agctccttcg tggcgccgtc gatgtcgccg agcggctgaa ggatcagctc gccgtcgagg
     1321 ccgctgcgct cccagcgacg catgccgagg cgcacggcgc cgacgtcgtc gctgatgtcg
     1381 aggctcagcg tcggtccgct ggcgacgagg tcgagcagca tctccgggtc ctcggcgaac
     1441 tcctgggtca gccgggtctc gagctcggac accgggagat cgcgcgcggc ggtcaaggga
     1501 ccgagaccgc cgccgatctc tcgcaatgag tagcagccga tcacgtcatc ctcgaggaac
     1561 acgatccgcc acgggaacgc ggccgcgggg gcgtggtgga ccggcagggc ctcgggcgcg
     1621 gcggcggcgt cgggctcgcc gggcaacggc gagtaggtga ccatgccctc catcatgtcg
     1681 cgggcgagcc cgccggcggt cagccacgcc tcgacctcgg cgcgcgggcg cgataacccg
     1741 cgcaccaggt acggcccgcg cgcgatcggt ggcagggaga ccagcgcctc ggcgcgcagc
     1801 caggtcgacg gcgccgggcc gacgccctcc atcaatgcgg cga
//
LOCUS       DASWNJ010001113         3246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_585513, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001113 DASWNJ010000000
VERSION     DASWNJ010001113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585513"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VGB85_18740"
     CDS             <1..525
                     /locus_tag="VGB85_18740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136135.1"
                     /translation="SSPGVQQDLRYAALTLNTAWYKALVGEYLSFAVRGVADLILGDA
                     PFYELTRFGALMPRDGPGGGWSLRGVPRQRYAGKAKLIQNLELRSLFWRFAIRQQRFV
                     LGAVAFLDTARIWADVHSTVLDGRNVDGGTFKVGTGGGLRVRWGETFLIRADAAYSPT
                     EGTPGFYVDIGHVF"
     gene            542..1909
                     /locus_tag="VGB85_18745"
     CDS             542..1909
                     /locus_tag="VGB85_18745"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0136136.1"
                     /translation="MPASPVAQDRRPPGPPLRRGLAQNLRYYAAFSTDPTGFVEQRFK
                     QYGDIYYAPATDGGLFVLKHPDHLRDLLITHAASYRKTHTALARLRTFLGDGLLTSDG
                     ADWTRQRRMVQPAFARTHLAGFARIMVEETDRTLTTWKGGETRDLGSNIMAMTLRSVS
                     RCLFGQDSAGEVDAVARAMLTFQAEFARVDILPPWLPTPQRLRFRRALATIDRIVFAM
                     IDAQRSDTTATSGHLLALLASAIDSEGDGAGLSREEVRDQLVTLFLAGHDTTSYALLW
                     TMTLLAAHPEATKLLTAELDSVLAGRLPTLEDLPRLTYTEQVIKEAMRLYPPAYVLGR
                     RAASDTEIGGYSVRAGSEVIAWIFMTHRDPRWFPEPEAFRPERFAPGAEANLPRLAYM
                     PFGAGPRACIGRQFAMIEAQLMLARIYQKFTLELAPGHKVEPKFRITLMPKHGLRARL
                     HARQP"
     gene            2034..>3246
                     /locus_tag="VGB85_18750"
     CDS             2034..>3246
                     /locus_tag="VGB85_18750"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEY0136137.1"
                     /translation="MASRKQLIKDVQEATRIAQESFGFDALAPGQQAAIRSLLSGRDT
                     LAVMPTGSGKSAIYQIPALIIPGPTVIVSPLLALQRDQLDSLQRTGVAEAAALNSNLR
                     ASERTAVLERLASGELEYLFLAPEQFGRDDLMTALLAAEPRLFVVDEAHCISEWGHDF
                     RPDYLRLGAVVDALGHPTVLALTATATPSVRAEIAERLHMREPEVLVTGFDRPDLWLG
                     VRIFETEPQKHAAFLAAMREATPPGIVYAATRKHTEDIAAALRDIGITAEAYHAGMAR
                     QARDDVHARFMADETAVIVATVAFGMGVDKPNVRFVYHYDISDSLDGYYQEIGRGGRD
                     GKGARALLFYRRADLGLHKFFAAGGQVNAEAVQQVVEAIAGSDDPVAASDLRDTTNLS
                     QTKVARAISRLA"
BASE COUNT          469 a         1370 c          944 g          463 t
ORIGIN      
        1 agctcgccgg gcgtgcagca ggacctgcgc tacgccgccc tgaccctcaa caccgcctgg
       61 tacaaggcgc tcgtcggcga gtatctgtcg ttcgcggtac gcggcgtcgc cgacctgatc
      121 ctcggcgacg cgcccttcta cgagctgacg cgcttcggcg ccctgatgcc gcgcgacggc
      181 cccggcggcg gctggtccct gcgcggcgtc ccgcgccagc gctacgccgg caaggccaag
      241 ctgatccaga acctcgagct gcgctcgctg ttctggcggt ttgcgatccg ccagcagcgc
      301 ttcgtcctcg gcgcggtcgc cttcctcgac accgcgagga tctgggccga cgtgcacagc
      361 accgtgctcg acggccgcaa cgtcgacggc ggcaccttca aggtcggcac cggcggcggc
      421 ctgcgcgtgc gctggggcga gacctttttg atccgcgccg acgctgccta ctcgccgacc
      481 gagggcaccc cgggttttta cgtcgacatc ggccacgtgt tttgagccta aactgcggcg
      541 catgcccgcc tcgcccgtcg cccaggaccg ccgcccgccc ggacccccgc tgcgccgcgg
      601 cctcgcccag aacctgcgct actacgccgc cttctccacc gacccgaccg gcttcgtcga
      661 gcagcgcttc aagcaatacg gcgacatcta ctacgcgccc gcgaccgacg gcggcctgtt
      721 cgtcctcaag caccccgacc acctgcgcga cctcctgatc acccacgccg ccagctaccg
      781 caagacccac accgccctcg cccgcctgcg aaccttcctc ggcgacggcc tgctgaccag
      841 cgacggcgcc gactggaccc gccagcgccg catggtccag cccgcgttcg cgcgcaccca
      901 cctcgccggc ttcgctcgca tcatggtcga ggagaccgac cgcaccctca ccacgtggaa
      961 gggcggcgag acccgcgacc tcggcagcaa catcatggcg atgaccctgc gcagcgtcag
     1021 ccgctgcctc ttcggtcagg actccgccgg tgaggtcgac gccgtcgccc gcgcgatgct
     1081 caccttccag gccgagttcg cccgcgtcga catcctgccg ccgtggctgc cgaccccgca
     1141 gaggctccgc tttcgccgcg cgctcgcgac gatcgaccgc atcgtcttcg ccatgatcga
     1201 cgcccagcgc agcgacacca ccgcgacgag cggccacctg ctcgccctgc tcgcctccgc
     1261 gatcgacagc gagggcgacg gcgccggcct ctcccgcgag gaggtccgcg accagctcgt
     1321 caccctgttc ctcgccggcc acgacaccac ctcgtacgcc ctgctgtgga cgatgaccct
     1381 gctcgccgcc caccccgagg cgaccaaact cctgaccgcc gagctcgaca gcgtgctcgc
     1441 cggtcgcctc ccgaccctcg aggacctccc gcgcctcacc tacaccgagc aggtgatcaa
     1501 ggaggcgatg cgcctctatc cgcccgccta cgtgctcggt cgccgcgccg cctccgacac
     1561 cgagatcggc ggctactccg tgcgcgccgg cagcgaggtc atcgcgtgga tcttcatgac
     1621 tcaccgcgac ccgcggtggt tccccgagcc cgaggcgttt cgccccgagc gcttcgcccc
     1681 cggcgccgag gccaacctcc cgcgcctcgc ctacatgccc ttcggggcag gtccacgcgc
     1741 ctgcatcggc cgccagttcg ccatgatcga ggcccagctg atgctcgccc gcatctatca
     1801 aaagttcacc ctcgagctcg cccccggcca caaggtcgag ccgaagtttc gcatcaccct
     1861 catgcccaaa cacggcctgc gtgcccgcct gcacgcgcgc cagccctgac ctccgcgccc
     1921 gctgcgcaac agtctcgcct ggctccctgg catcgcgccg cgcgcaccga agccgcgcgc
     1981 ggccacctca tcccgccagc cctcgatcgc ccgcctgcta cacgagcccg acgatggcct
     2041 cgcgcaagca attaatcaaa gacgtccagg aggccacgcg catcgcccag gagagcttcg
     2101 gcttcgacgc cctcgcccct ggccagcagg ctgcgatccg ctccttgctg tccggacgcg
     2161 acaccctcgc cgtcatgccg accggctccg gcaagtccgc gatctaccag atccccgcgc
     2221 tgatcatccc cggcccgacc gtcatcgtct cgccgctgct cgccctgcag cgcgaccagc
     2281 tcgactccct gcagcgcacc ggcgtcgccg aggccgccgc cctcaactcc aacctccgcg
     2341 ccagcgagcg caccgcggtc ctcgagcgac tcgcctccgg tgagctcgag tacctgttcc
     2401 tcgcccccga gcagttcggc cgcgacgacc tgatgaccgc gctgctcgcc gccgagccgc
     2461 gcctgttcgt cgtcgacgag gcccactgca tcagcgagtg gggtcatgac tttcgccccg
     2521 actacctgcg cctcggcgcc gtcgtcgacg cgctcggcca cccgaccgtg ctcgccctga
     2581 ccgccaccgc cacgccctcc gttcgcgccg agatcgccga gcgcctacac atgcgcgagc
     2641 ccgaggtcct cgtcaccggc ttcgaccgcc ccgacctgtg gctcggcgtc cgcatcttcg
     2701 agaccgagcc gcaaaagcac gccgccttcc tcgcagccat gcgcgaggcc accccgcccg
     2761 gcatcgtcta cgccgccacc cgcaagcaca ccgaggacat cgccgccgcc ctgcgggaca
     2821 tcggcatcac cgccgaggct taccacgccg gtatggcccg ccaggcccgc gacgacgtgc
     2881 acgcccgctt catggccgac gagaccgccg tcatcgtcgc cacggtggcc tttggcatgg
     2941 gcgtcgacaa gcccaacgtg cgctttgtat atcactacga catcagcgac tcgctcgacg
     3001 gctattatca ggagatcggc cgcggcggtc gcgacggcaa gggcgcgcgc gccctgctgt
     3061 tctatcgccg cgccgacctc ggcctgcaca agttcttcgc cgcgggcggc caggtcaacg
     3121 ccgaggccgt ccagcaggtc gtcgaggcga tcgccggcag cgacgacccg gtcgccgcga
     3181 gcgacctgcg cgacaccacc aacctgtcgc agaccaaggt cgcccgcgcg atcagccgcc
     3241 tcgccg
//
LOCUS       DASWNJ010001114         6539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_125243, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001114 DASWNJ010000000
VERSION     DASWNJ010001114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6539
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125243"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            511..1017
                     /locus_tag="VGB85_18755"
     CDS             511..1017
                     /locus_tag="VGB85_18755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0136138.1"
                     /translation="MLKTLQKSYASATNLSVQRMARLLIVEDDELSRDIATRWLTRHG
                     YNNVESAACGAEGLELCTDDPPDILILDHSLPDLGGMAIAEHLCLNFRREERPWIVLF
                     TAAADSTINRLMSTGYFDDLLRKPCVGEDYVAALARAHAGLRERRKLAAKNNMMINPG
                     SSSTSLHL"
     gene            1263..1901
                     /locus_tag="VGB85_18760"
     CDS             1263..1901
                     /locus_tag="VGB85_18760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136139.1"
                     /translation="MHLPKSSILFSLVLLGFGCDSGNKDTAATAGTAGTAGTATAGAS
                     TAGSTAGASTAGSTASGSTAGRTTAGDAGGSAAGSTAGSTAGSTAGGSMGAGGAGGSM
                     GAGSAGGTAGSGAGSTAGGSTAGSAGGTMGAGSAGGTAGSGSGSTAGGSTAGGSTAGG
                     STAGSAGGSAGGTAGTAGGSAGGSGSTAGGTAGGTAGGTAGGGSGSKTGGSH"
     gene            2045..2833
                     /locus_tag="VGB85_18765"
     CDS             2045..2833
                     /locus_tag="VGB85_18765"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0136140.1"
                     /translation="MSSVTIPDRPALVAAHGLGLRHPGGVLALDGVDLEVQPGEFVAL
                     VGPSGCGKSTLLRAISGLVPTSSGRLEVAGMAPAAARKQWGRLAFVFQDPTLLPWRTV
                     RDNVALPLELGASTDTADLGRAVDRALELVGLGDFAGTHPAELSGGMRMRASLARALV
                     TRPELLLLDEPFGALDELTREHLDDELLRLWQRDGFAAIAVTHSLAEAIWLADRVLVM
                     SPRPGRILHILPVPLPRPRPPDLLTSPEFTALLATLRTYVQRAA"
     gene            2830..3612
                     /locus_tag="VGB85_18770"
     CDS             2830..3612
                     /locus_tag="VGB85_18770"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0136141.1"
                     /translation="MSEPLHVRILPPLVVFAGVLALWQLLCVALALPAYLVPAPTRVA
                     AAALAGAGPLTAATALTAAAALLGLLISLLLGTAIAVLFSQSRLLARSLYPYAIFLQT
                     VPVVAVAPLIVIWFGTGLASVVLVSVMISLFPVITNGTTGLTRVDPQYLELFAMRDAS
                     RLQILIKLRLPGAVPYLVTGARISSGLAVIGAIVGEFFAGYGAAGYGLGYVILQTSGQ
                     LKTAELFAAIFACTLLGLVLFAAVSLLGELVLRRWRGRESEA"
     gene            3609..4022
                     /locus_tag="VGB85_18775"
     CDS             3609..4022
                     /locus_tag="VGB85_18775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136142.1"
                     /translation="MSARATDDEVAAILADPDKRISGELRWADDEDASGQEFLVNVAC
                     AAAELFVRGQRCAAAGTLRFALISRGLGRIAALDLAPGDLHVHRWSPGRRDEVVPLTD
                     AASDELAAWRLFCRLTEISHDGVLACAPPTQESLL"
     gene            4019..5725
                     /locus_tag="VGB85_18780"
     CDS             4019..5725
                     /locus_tag="VGB85_18780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0136143.1"
                     /translation="MSAPPDPEDRLRAGLAAPLFTADEWGAHLRVHTPFRLPDGDPLV
                     LFLLRHSDPPLLGDLGETLRWLRSHTIEPRRSARQYQQIADACASLGVTLERGELRTT
                     TRPDESLAAAAVRVAQACLRVADLRRAGRPRITRSFPDEVESYLRATLPGPLAITRND
                     RIPGISATTWSVDFRVRTPERHLLLFLLANERRDQARKLAEHVVAACTDLAHLRKLRR
                     MPVQFITVFDDRGAAWNDGDARLVAPFATPHRWSERATWVPALADPELGPDLEDPDDD
                     DNEDEPEEHSDDEATDPREDPPMTLHLRPATPDDYPEFARLFPQLGTSDPVPDEDVWT
                     GHLHASTLMLVEGAQLLGYAYYERMEEFGYVRNVVIDAAHRGRGHGRTLMLRLAAHLR
                     ALGCTRWCLNVEAANTVAIALYRSLGLDVAYRSAALRFLWSAVESLPGEDLALEVVPV
                     DPSEDAEIEAAHELPSGQLATIREDPTRILRRLHDPADPTALALGIASFNPHFPGAYP
                     FRVVRPGLARALLLGLRPHALPDEPRMHVVVEDDEPLRALLLEIGAVLRLQLLFLRGD
                     LP"
     gene            6333..>6539
                     /locus_tag="VGB85_18785"
     CDS             6333..>6539
                     /locus_tag="VGB85_18785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136144.1"
                     /translation="MLSTITLLASTLLVAPNFTAPPPAATHGLGGIDISIGDKGDDLD
                     LDLDLDACLDLDLDVNLDCEIEVGA"
BASE COUNT          902 a         2581 c         2084 g          972 t
ORIGIN      
        1 ggcccgcggc tgcgcgggcc gcttgctgtg gaccgcgatc gggtgaccgt cgaccgtcac
       61 cgtgtgccgc cgcaacacgt tctcggcggc gagcgcgggc gtcgtgctcg cctccgaggg
      121 cccgccgcga acgcaggcgc aaaacaacag cacgaacagc aggcggcccg gcgacatgcg
      181 gcgaatgata ggcggtcgag tcagcttcgg gagatctatg agccgatggt tcatgacggg
      241 gccggcgggt ttgacaggag tcccgtgaat tgagcagcct cggcagtcct ggtgccacag
      301 aacagtgttc aagagcgcgt attgccagcc tcgatccggc gcgccctgcg aaccgaggcc
      361 acctgcggcc cgcttttgaa attcgtcata tcggctcatc cggcatatcg cgggtatttc
      421 tgcggtgggt cgaccgtggt ggctctggcg cagcctcgct ccctcctcgg cgtggctgcg
      481 gtcgaccaaa cgcgggatgc ttcgatcacc gtgctcaaga cgctccagaa gtcctacgcc
      541 tctgcgacca acctgtccgt gcagcggatg gcccgtctgc tcatcgtcga ggacgacgag
      601 ctctcgcgcg acatcgccac tcgctggctg acgcgccacg gttacaacaa cgtcgagagc
      661 gcggcctgcg gcgccgaggg cctcgagctg tgcaccgacg acccgccgga catcctgatc
      721 ctcgaccact cgctgcccga cctcggcggc atggcgatcg ccgagcatct ctgcctcaac
      781 ttcaggcgcg aggaacggcc gtggatcgtg ctgttcacgg ccgccgccga ctcgacgatc
      841 aaccgcctga tgagcaccgg ctacttcgac gacctgctgc gtaagccctg cgtcggcgag
      901 gactatgtcg cggcgctcgc ccgggcccac gccggcctgc gagagcgtcg caagctcgcc
      961 gcgaagaaca acatgatgat caaccccggc agctcgagca cctcgctgca cctctgagca
     1021 gtcgcgtcga gaccagggtt tacgatcatt cgccggtagc acgagacggc caaagcggcc
     1081 gcgtgatggg ggcagtcagg atggtggcat ctgcgcacaa ggcggccaga tgccacccct
     1141 cgctgccccc tcctcgttcc gctggcccat gccacgcgcc gaactcggca gtttgtgcga
     1201 cgagcctcgc cgcccccgac cggcgactgg cacgattcgc ggatctcgta caggagagcg
     1261 ccatgcatct ccccaagtct tcgatcctgt tctccctcgt ccttctcggc ttcggctgcg
     1321 acagcggcaa caaggacacc gcggccacgg ccggtaccgc cgggaccgcc gggaccgcca
     1381 ccgcgggggc ctcgaccgct ggcagcaccg ccggagcctc gaccgctggt agcaccgcct
     1441 cgggcagcac cgccggccgc accaccgccg gtgacgcggg tggcagcgcc gcgggtagca
     1501 ccgccggtag caccgctggc agcaccgccg gcggctccat gggcgccggc ggtgccggtg
     1561 gcagcatggg cgccgggtcg gccggtggca ccgcgggctc cggcgcgggc agcaccgccg
     1621 gtggcagcac cgcgggctcc gctggcggca ccatgggcgc cggttcggcc ggtggcaccg
     1681 cgggctccgg ctcgggcagc accgccggtg gcagcaccgc gggtggcagc accgcgggtg
     1741 gcagcacggc cggttcggcc ggcggctccg cgggcggcac cgccggcacc gcgggcggct
     1801 ccgccggtgg ttcgggcagc accgccggtg gcactgccgg cggcaccgct ggtggcacgg
     1861 ccggtggcgg ttcgggcagc aagaccggcg gaagccacta atccttcgcc gcggctcgcc
     1921 gcttcatcga ccgggcgtcc cgggtcgtcg agtcactcga cgcccgtggg cgcctttgcc
     1981 tttgccgcgc ccgggcccct gagattcgca ggccgtggcg cgggcgtgat aacttctcgc
     2041 cgcggtgtcc tccgtgacca tccccgatcg gccggccctg gtggcggccc acggcctcgg
     2101 tctgcgtcac ccggggggag tgctcgcgct cgacggcgtc gacctcgagg ttcagcccgg
     2161 tgaattcgtc gcgctcgtcg gcccgtccgg ctgcggcaag tcgaccctgc tgcgagcgat
     2221 ctccggcctc gtcccgacct cgtccggccg cctggaggtc gccggcatgg cccccgccgc
     2281 cgcccgcaaa caatggggcc gcctcgcctt cgtgttccag gacccgaccc tgctgccctg
     2341 gcgcaccgtc cgcgacaacg tcgccctccc gctcgagctc ggcgcctcga ccgacaccgc
     2401 cgacctcggc cgtgccgtcg accgggccct cgagcttgtc ggcctcggcg acttcgccgg
     2461 cacccacccc gccgagctgt ccggcggcat gcgcatgcgc gcctcgctcg cgcgcgcgct
     2521 cgtcacccgc cccgagctgc tgctgctcga cgagcccttc ggagccctcg acgagctgac
     2581 ccgcgagcac ctcgacgacg agctgctgcg cctgtggcag cgcgacggct tcgccgcgat
     2641 cgccgtcacc cacagcctcg ccgaggccat atggctcgcc gaccgcgtcc tcgtcatgag
     2701 cccgcgaccg ggccgcatcc tgcatatctt gccagtcccg ctgccgcgac cgcgaccgcc
     2761 cgacctcctg accagcccgg agttcaccgc gctgctcgcc accctgcgca catatgtcca
     2821 gagggccgcg tgagcgagcc gctgcatgtg cgcatattgc cgccgctggt cgtcttcgcc
     2881 ggcgtgctcg cgctgtggca gctgttgtgt gtcgcgctcg cgctgccggc ctacctcgtg
     2941 cccgcgccga cgcgggtcgc cgccgccgcg ctcgcgggcg ccggtcccct gacggccgcg
     3001 acggccctga ccgccgccgc cgcgctgctc ggcctgctga tcagcctttt gctcggcacc
     3061 gcgatcgccg tgctgttctc gcagtcgcgc ctgctggccc gcagcctcta cccctacgcg
     3121 atcttcttac agacagtccc ggtcgtcgcc gtcgcgccgt tgatcgtcat atggttcggc
     3181 accggcctcg ccagcgtcgt gctcgtatcg gtgatgatca gcctgttccc tgtgatcacc
     3241 aacggtacca ccggcctgac gcgcgtcgac ccgcagtacc tcgagctgtt cgccatgcgc
     3301 gacgcctcgc gcctgcagat tttaataaaa ttgcggctgc ccggcgcggt cccctatctc
     3361 gtcaccggcg cgaggatcag cagcggactc gccgtgatcg gcgcgatcgt cggggagttc
     3421 ttcgccggct acggcgcggc cggttacggc ctcggctacg tcattcttca aacttccggc
     3481 cagctcaaga ccgccgagct gttcgcggcg atcttcgcct gcaccctcct cgggctggtc
     3541 ctcttcgccg ccgtgtcgct gctcggcgag ctcgtccttc gtcgctggcg cggccgcgag
     3601 tccgaggcat gagcgcgcgc gccaccgacg acgaggtcgc tgcgatcctc gccgaccccg
     3661 acaagcggat ctccggcgag ctgcgctggg ccgacgacga ggacgccagc ggccaggagt
     3721 tcctggtcaa cgtcgcctgc gcggccgccg agctgttcgt ccggggccag cgctgcgccg
     3781 ccgcgggcac cctgcgcttc gcgctgatct cccgcggact cggccggatc gccgccctcg
     3841 acctcgcccc cggcgacctc cacgtccacc gctggagccc cggccggcgc gacgaggtcg
     3901 tcccactcac cgacgcggcc agcgacgagc tcgccgcctg gcgcctgttc tgccgcctga
     3961 ccgagatctc ccacgacggc gtgctcgcct gcgccccgcc gacccaggag tcgctgctgt
     4021 gagcgcgcca cctgacccgg aggacaggtt gcgcgccggc ctcgcggccc cgctgttcac
     4081 cgccgacgag tggggcgccc acctccgcgt ccacacgccg tttcgcctgc ccgacggtga
     4141 cccgctggtg ctgttcctgc tgcgtcacag cgacccgccc ttgctcggcg acctcggcga
     4201 gaccctgcgc tggctgcggt cgcacacgat cgagccgcgc cgcagcgccc ggcaatacca
     4261 gcagatcgcc gacgcctgcg cgagcctcgg cgtcacgctc gagcgcggcg agctgcggac
     4321 gacgacgcgc cccgacgagt cgctcgctgc ggccgcggtg cgcgtcgccc aggcctgcct
     4381 gcgcgtcgcc gacctgcgcc gcgccggtcg cccgcgcatc acgcggtcgt tccccgacga
     4441 ggtcgagtcc tacctgcgcg cgaccctccc cggcccgctg gcgatcaccc gcaacgaccg
     4501 catccccggc atctcggcga ccacgtggag cgtcgacttt cgcgtgcgca cccccgagcg
     4561 ccacctgctg ctgttcttgc tcgccaacga gcgccgcgac caggcccgca agctcgccga
     4621 gcacgtcgtc gccgcctgca ccgacctcgc ccacctgcgc aagctccggc gcatgccggt
     4681 gcagttcatc accgtcttcg acgaccgcgg cgccgcctgg aacgacggcg acgcccgcct
     4741 ggtcgcgccc ttcgccacgc cgcaccgctg gagcgagcgc gccacctggg tccccgccct
     4801 cgccgacccc gagctcggcc ccgacctcga agatcccgac gatgacgaca atgaggacga
     4861 gcccgaggag cacagcgatg acgaagcaac cgacccccgc gaggacccgc cgatgaccct
     4921 gcacctgcga ccggcgaccc cggacgacta ccccgagttc gcccgcctgt tcccccagct
     4981 cggcaccagc gaccccgtgc ccgacgagga cgtctggacc ggccacctgc acgcgagcac
     5041 gctgatgctc gtcgagggcg cgcagctcct cggttacgcg tactacgagc gcatggagga
     5101 gttcggctac gtgcgcaacg tcgtcatcga cgccgcccac cgcggccgcg gccacggtcg
     5161 cacgctgatg ctgcgcctcg ccgcgcacct gcgcgcgctc ggctgcacgc gctggtgcct
     5221 caacgtcgag gccgccaaca ccgtcgccat cgccctgtac cgcagcctcg gcctcgacgt
     5281 cgcctatcgc tcggccgcgc tgcgcttcct gtggtccgcg gtcgagtccc tgcccggcga
     5341 ggacctggcc ctcgaggtcg tgccggtcga cccctccgag gacgccgaga tcgaagccgc
     5401 ccacgagcta ccgagcggac agctcgccac gatccgggag gacccgacgc gcatcctccg
     5461 gcgcctgcac gaccccgccg acccgaccgc gctcgccctc ggcatcgcca gcttcaaccc
     5521 gcacttcccc ggcgcctatc cgttccgcgt cgtccgcccg ggcctcgccc gcgccctgct
     5581 gctcggcctg cgcccccacg cgctcccgga cgagccgcgc atgcacgtcg tcgtcgagga
     5641 cgacgaacct ctgcgcgcgc tgctgctgga gatcggcgcc gtgctgcgcc tccagctgct
     5701 gttcctgcgc ggcgacctgc cctgacaccg gcaatatggc cggtttacgc cggctatgtc
     5761 aggagcttcg ccctgcgcgc ggatcgccgg atcacataca ttgcgcggcg gacccggacc
     5821 gcctcataaa gctgacacgc attcacaggg ggcaaatgac cccgtgttgc attttttcaa
     5881 caaacggccc cggagcacac caggacgccg cgggacgcgc cgggctcact gcagcgaaac
     5941 agtttttgtt tcaagtactt gcgcgcgatc cagccagacc gcggcgcgcg cagcggcgcg
     6001 agctatgcga gatatcgccg cgccgaactg cggcgttaac aatacacatg tagctttgcg
     6061 acgcgggcga aaactcgcga agtgacagca tttgcgcccc acgagggcgc cgatagaaaa
     6121 tcctcaattg gccgccaaac acgattccgt ggcctctgat agccgatctg ccactcggcg
     6181 ccgaacacgc gagacattgc cgccggctcg tcgtcctggc cgcgagagct tcgcccgcca
     6241 tactcgaatc atcgaagccg cgacggaatc ccccggcggc gccccgacaa gacactcgcc
     6301 ttcgacacgt tcgaagcaaa aggaatcacc caatgctctc caccatcacc ctgctcgcct
     6361 ccaccctcct cgtcgccccg aacttcaccg cgcccccccc ggccgcgacc cacggcctcg
     6421 gtggcatcga catcagcatc ggcgacaagg gtgacgacct ggacctggac ctcgacctgg
     6481 acgcctgtct ggacctcgac ctcgacgtga acctggactg cgagatcgag gtcggcgca
//
LOCUS       DASWNJ010001115         1557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_20630, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001115 DASWNJ010000000
VERSION     DASWNJ010001115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20630"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1163
                     /locus_tag="VGB85_18790"
     CDS             <1..1163
                     /locus_tag="VGB85_18790"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY0136145.1"
                     /translation="PRSVLLAGFVALPACYVGLDGEPPEGTTLATSTGGAGTSTGAAS
                     DPTASSAAPTGEPTTGAEHTSTGPVTATVPGTTDMSGGTGGTTGLDTGGEPGTSEPGT
                     TEPGTTGMTGDPPPDEAAMLCARWNDDRAQLGEGTWSGDVDSCNKGGVSADGLANTLR
                     LVNLYRFIADLPPVVEDAARSGKAQSCALMMHANGTLNHSPPMNWKCYAAEGAEAAGQ
                     SNIAGAPGVFGIDLYMVDDGNTTTIGHRRWILSNSLGPIGMGSTSQYSCLLVIGGNGN
                     AGKAWTAWPPPGLVPLQAMHVPTVGWSDVDKAGWSVQSDSIDLGGATVSVTEGGVDRP
                     VAVNVLGQGYGSTHAIRFVPQGWAVEAGKTYDVTLGNVSQPIAYSVAIVDCG"
     gene            complement(1133..>1557)
                     /locus_tag="VGB85_18795"
     CDS             complement(1133..>1557)
                     /locus_tag="VGB85_18795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0136146.1"
                     /translation="DRLPPLPIVADPTRVRQIVYNLVANALKFTHRGSIELAVELADD
                     TYFVHVADSGIGIAPEKLALLFHNFSQVHDEDAGEYGGTGLGLAISRRYARLMGGDIV
                     AESEPGRGSRFTVRLPAPTPARTGAEASGQPQSTMATL"
BASE COUNT          238 a          521 c          597 g          201 t
ORIGIN      
        1 cgccgagatc tgtgctgctc gccggtttcg tggccttgcc cgcctgttac gtcgggctcg
       61 acggcgagcc gcccgagggc acgaccctcg ccaccagcac cggtggcgcc ggcacgtcga
      121 ccggcgcggc gtccgacccg acggcgagca gcgcggcgcc gaccggcgag ccgaccaccg
      181 gcgcggagca cacgagcacc gggccggtga cggcgaccgt gcctggcacg acggacatgt
      241 ctggagggac aggcggcacg accggcctcg acaccggcgg cgagcccggc acgagcgagc
      301 ccggcaccac ggagccgggc acgaccggga tgaccggcga cccgccgccg gacgaggcgg
      361 cgatgttgtg tgcgcgctgg aacgacgacc gcgcgcagct cggcgagggc acgtggagcg
      421 gggacgtcga cagttgcaac aaggggggcg tcagcgcgga cggtctggcg aacacgctgc
      481 ggctggtcaa cctgtaccgc ttcatcgccg acctgccgcc ggtggtcgag gacgctgcgc
      541 gcagcggcaa ggcgcagtca tgcgccctga tgatgcacgc caacggcacg ctcaatcatt
      601 caccgccgat gaactggaag tgttacgcgg cggagggcgc ggaggcggcg ggtcagagca
      661 acatcgcggg cgcgccgggc gtgttcggca tcgacctcta catggtcgac gacggcaaca
      721 ccacgacgat cggtcaccgg cggtggatcc tttcaaatag cctgggaccg atcgggatgg
      781 gctcgacctc gcagtactcg tgcctgctgg tgatcggggg caacggtaac gccggcaagg
      841 cgtggacggc atggccgccg ccggggctgg tgccgttgca ggcgatgcac gtgccgacgg
      901 tcggctggag cgacgtcgac aaggccggct ggagcgtgca gagcgacagc atcgacctcg
      961 gcggcgcgac cgtgtcggtc accgagggcg gcgtcgaccg gccggtcgcc gtcaacgtgc
     1021 tcggccaggg ctatgggtcc acgcacgcga tccgcttcgt gccgcagggc tgggcggtgg
     1081 aggccggcaa gacttatgat gtcacgctcg gcaacgtgtc gcagccgatc gcctacagcg
     1141 tggccatcgt cgactgcggc tgaccggacg cctcggcgcc cgtgcgcgcg ggcgtcggcg
     1201 cgggcaggcg gaccgtgaag cgggagccgc ggcccggctc gctctcggcg acgatgtccc
     1261 ctcccatcaa gcgcgcgtag cgacggctga tcgccaggcc gaggccggtg ccgccgtact
     1321 cgccggcgtc ctcgtcgtgg acctgcgaga agttgtggaa cagcagcgcg agcttctccg
     1381 gcgcgatgcc gatgccggag tccgccacgt gcacgaagta cgtgtcgtcg gcgagctcga
     1441 ccgcgagctc gatgctgccg cggtgcgtga acttcagggc gttggcgacc aggttgtaga
     1501 cgatctggcg cacgcgggtc gggtcggcga cgatcggcag cggcggcagg cggtcga
//
LOCUS       DASWNJ010001116         2320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_443627, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001116 DASWNJ010000000
VERSION     DASWNJ010001116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443627"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VGB85_18800"
     CDS             <1..822
                     /locus_tag="VGB85_18800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136147.1"
                     /translation="PALNSLTRTLLDARVARIELAIREPRVPADSGDVVLQLPLELLR
                     ETDQGPASGALRKARLHMHLSGRGARLAADGKWLDLRPGAPELEAQLALLRRAYPREH
                     MITIGLADDVLYQQLVDLLRALIGGPQRRFEVAAWRPAAAAPPPEPAAKATAAEERRL
                     QRRAELGGDTARAALQQPFPLAAGDQPRLEALARQLLRCLPELETPLAPGERVLVALR
                     FDEGRLAEVTAQKPRTRVPAERLAALQTCAEAETRGFRLREHRDAIRSEVVLVAR"
     gene            907..1764
                     /locus_tag="VGB85_18805"
     CDS             907..1764
                     /locus_tag="VGB85_18805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136148.1"
                     /translation="MPRLSHEALVQLVRNAPGLIPALLGGAGPGQDVRPVHVTAAEFT
                     DLNFAEHRADVVLTVGGTPDRPDEAFVVEVQTDPDPQKRVTWPLYVAGARTRLGCPVT
                     LVVLALDPAVARWCARPIDLGRGRCVLHPWVLGPADIPAITDLEQARAAPELAVLSVA
                     AHADEPGAEHIALAALTAARMLDNNRAVLYPDFVVALLGAAARTALEKLMHVTRYEFQ
                     SDFARHYYGEGIAQGKAETLLKLLQLRGFVVPEAEQARVLACRDLDRLDTWVARVLTA
                     RDLDEVLAD"
     gene            1946..>2320
                     /locus_tag="VGB85_18810"
     CDS             1946..>2320
                     /locus_tag="VGB85_18810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0136149.1"
                     /translation="MTRPPPRDLEQTSTPPALAARPALWDEVTHASRTATPAMHVTDS
                     EDGLGRARIGRYTVLRKLGSGGMGVVYAAFDDSLDRKVAIKVLRETSDDPQDQARLLR
                     EAQALARLSHPNVVTVHDVGTTD"
BASE COUNT          284 a          983 c          760 g          293 t
ORIGIN      
        1 ccggccctca actcgctgac gcgcaccttg ctcgacgccc gcgtcgcccg catcgagctg
       61 gcgatccgcg agccacgggt gcccgccgac agcggcgacg tcgtgctgca actcccgctc
      121 gagctgctcc gcgagaccga ccagggcccg gcctccggcg cgctacgcaa ggctcgcttg
      181 cacatgcacc tctcgggtcg cggcgctcgc ctggccgccg acggcaagtg gctcgacctg
      241 cgccccggcg cgcccgaact cgaggcgcag ctggccctgc tgcgccgcgc ctacccgcgc
      301 gagcacatga tcacgatcgg cctcgccgac gacgtgctct accagcagct cgtcgacctc
      361 ctgcgcgcct tgatcggcgg gccgcagcgg cgcttcgagg tcgccgcctg gcgcccggcc
      421 gccgccgcgc caccgcccga gccggccgcg aaggcgaccg ccgccgagga gcgccgcctg
      481 caacgccgcg ccgagctcgg cggcgacacg gcccgcgccg cgctgcagca gccgttcccg
      541 ctcgccgcgg gcgatcagcc gcggctggag gcgctcgccc gccagctgct gcgctgcctg
      601 cccgagctcg agaccccgct cgcgcctggc gagcgggtgc tcgtcgcgct gcgcttcgac
      661 gagggtaggc tcgccgaggt caccgcgcaa aagccccgca cgcgcgtgcc ggccgagcgc
      721 ctcgccgccc tgcagacctg cgccgaggcc gagacccgcg gctttcgcct gcgcgagcac
      781 cgggacgcga tccgcagcga ggtcgtgctc gtcgcccgct gacacctgtc ctcggggaca
      841 ggtgctgcgc atgagctgcg accgcggcgt gcattcgcac ccgcgccgcg ctagcgttcg
      901 cgcccgatgc cccggctgtc ccacgaggcg ctcgtccagc tggtgcgcaa cgccccgggc
      961 ctgatccccg cgctgctcgg cggggctggc ccgggccagg acgtgcgccc ggtccacgtg
     1021 accgcggccg agttcaccga cctcaacttc gccgagcacc gcgccgacgt cgtgctgacc
     1081 gtcggtggca cgccggaccg ccccgacgag gccttcgtcg tcgaggtcca gaccgatccg
     1141 gacccgcaaa agcgggtcac ctggccgctc tacgtcgccg gcgcgcgcac gcggctcggc
     1201 tgcccggtga cgctggtggt cctcgcgctc gacccggccg tcgcccgctg gtgcgcccgg
     1261 ccgatcgacc tcgggcgcgg ccgctgcgtc ctccatccgt gggtcctcgg ccccgccgac
     1321 atccccgcga tcaccgacct cgagcaggcg cgcgccgccc ccgagctcgc ggtgctctcg
     1381 gtcgccgccc acgccgacga gccgggcgcc gagcacatcg ccctcgccgc gctcaccgcg
     1441 gcccgtatgc tcgacaacaa ccgcgccgtc ctctaccctg acttcgtcgt cgccctgctc
     1501 ggcgcggcgg cccgcaccgc cctggagaag ctcatgcacg tcacccgcta cgagtttcaa
     1561 agtgacttcg cccggcacta ctacggcgag ggcatcgccc agggcaaggc cgagaccctg
     1621 ctcaagctgc tccagctgcg cggcttcgtc gtcccggagg ccgagcaggc gcgcgtgctc
     1681 gcttgccgcg acctcgaccg cctcgacacc tgggtcgccc gcgtgctgac ggcccgcgac
     1741 ctcgacgaag tcctcgcaga ttgacgacct cacggtcccg cccgtggacc acgacctcac
     1801 gggcccgccc gtggaccact tcctcacggg cccgcccgtg gacccatgtc gcctccggga
     1861 cccacgcgtg gtcgcccgac ctgtcctcga ggccaccccg cctttctgcc cgccgccgcg
     1921 gcgtgtgggg taggctgcgg ttgcgatgac gcggccgcca ccccgcgacc tcgaacagac
     1981 gtcgaccccg ccggccctgg ccgcgcgccc ggccctctgg gacgaggtca cccatgcttc
     2041 gcgcaccgcg acgccggcga tgcacgtcac cgactccgag gacggcctcg gacgcgcgcg
     2101 gatcggccgc tacaccgtcc tgcgcaagct cggcagcggc ggcatgggcg tcgtctacgc
     2161 cgctttcgac gactcgctcg accgcaaggt cgcgatcaag gtcctgcgcg agaccagcga
     2221 cgacccgcag gaccaggccc gcctgctgcg cgaagcccag gcgctcgccc gcctgtcgca
     2281 ccccaacgtc gtcaccgtgc acgacgtcgg caccaccgac
//
LOCUS       DASWNJ010001117         4849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_402800, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001117 DASWNJ010000000
VERSION     DASWNJ010001117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402800"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VGB85_18815"
     CDS             complement(<1..564)
                     /locus_tag="VGB85_18815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136150.1"
                     /translation="MTALPALALALAAGACNLTPAMTDATTDDAATSSSTGGTATTGD
                     TSDTTPTTDNTTPTSNPSEPTTEPTTEPTTNSTDPTTDPTTETAGEDATIYDIQQEKI
                     AVDTVVTVKGVIVTSPVKVKDSKGTMFIEEPAGGEYSGIAVYMYDEVTAALDAPPGSV
                     VNVTGTVSDFFGNSQLVVMAPGDIEVVG"
     gene            complement(755..828)
                     /locus_tag="VGB85_18820"
     tRNA            complement(755..828)
                     /locus_tag="VGB85_18820"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(792..794),aa:Arg,seq:acg)
     gene            complement(950..1780)
                     /locus_tag="VGB85_18825"
     CDS             complement(950..1780)
                     /locus_tag="VGB85_18825"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY0136151.1"
                     /translation="MVHSPQVTPEQAQEPDPAGGPGPAGPGGLAAGVSAATSEILLAV
                     FAFVLAFALLLAIPGVQPAIFWVLDATFGRPLLSPDKVRQVHGIVLTPLATLAAALLY
                     GWLGRRVDGLSAPPPAVWRVAGPAATAGQVVLHVLLAIAGSYVLALLMHLLGAPVSEQ
                     RLVLDLVAGGDLRRPELATLALSALVLAPVGEELFFRGQLMRRTWQRGGPVAAYLASA
                     LLFAAFHGNLQGFVVYTWLGLVFGLVYARTGRIGAAMVVHFGNNALTLAMLLLTPPAP
                     "
     gene            1921..2009
                     /locus_tag="VGB85_18830"
     tRNA            1921..2009
                     /locus_tag="VGB85_18830"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1955..1957,aa:Ser,seq:gct)
     gene            complement(2061..3416)
                     /locus_tag="VGB85_18835"
     CDS             complement(2061..3416)
                     /locus_tag="VGB85_18835"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0136152.1"
                     /translation="MLLIVEDHEDTRETLRDCLEEFGYTVDTAADGQEGLAAVRQKSF
                     DLVITDLNMPRMDGLGMLQAIRRETQGPDLLMLSADGQISRAVDAMRLGAINYLVKPV
                     ELDALVTEVRAVMQARRDRASEHDAVETVTVMGHHTRPLQADPAATLDPRVRGNSSED
                     EALPRTIGRYQVLSELGSGGMGTIFKCFDPALARTVAVKVVADDSDHGGRDDLLRRFA
                     CEAQAAGMLRHPNIVTVYDYSADPEGPVYLAMEYLAGTSLAALLASQGKLPWRRAASI
                     GFQLADALEFAHRNQVIHRDVKPANVFILEGDVAKILDFGIAKLANSELTVPGTFIGS
                     PRYLSPESFRGARIDYRADQFSLGSLMYEMVAGVPAFDFTDFYAGVHHILSEDPRPLE
                     SLVPDAPSLLYDIVERLHQKDPDRRYHNEMRLLDDLLELTEIAGMTLHLGVTRDMSGY
                     R"
     gene            3568..4230
                     /locus_tag="VGB85_18840"
     CDS             3568..4230
                     /locus_tag="VGB85_18840"
                     /inference="COORDINATES: protein motif:HMM:NF013158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136153.1"
                     /translation="MRLLRLTAPLLFALTAAWLLPAPASACSCVPPPPPRGAFAEATA
                     VFEGKVARLDEDPATHRRTATLEVARVWKGAVTASTRVATRDAGAMCGFEFTVGQSYL
                     IYAEGEAASLSTSLCSRSAPSSKAKADFAALGPGKPPPPGQPEPAEPVKPVEPVKPVK
                     PVKPVKPVEPVKPVEPVKPVEPVTPVEPAKTAGCAVGGHGGDAGGLAVLVLFAGRRRR
                     PG"
     gene            complement(4238..>4849)
                     /locus_tag="VGB85_18845"
     CDS             complement(4238..>4849)
                     /locus_tag="VGB85_18845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0136154.1"
                     /translation="RLAGASPSGYRASGGEHAAVIPIHDAPAARPGDDSERADDRAHR
                     ARWAGTLADHGRGISRMAGSYTRGAAEREDLEQDIALALWQALPTFRGESSLKTFAYR
                     VARYCCFRHVRRRKDLEDATDPATVADPGACIESAILRADQRARIEQALTDLPDTLES
                     VLTLHLTGRSYAQIAETLGISERNVSVRLSRARAHLRRQLVAA"
BASE COUNT          721 a         1576 c         1857 g          695 t
ORIGIN      
        1 gccgacgacc tcgatgtcgc cgggggccat cacgaccagc tggctgttgc cgaagaagtc
       61 gctgacggtg ccggtgacgt tgacgacgct gccgggcggg gcgtcgagcg cggccgtgac
      121 ctcgtcgtac atgtacacgg cgatgccgga gtactcgccg cccgcgggct cctcgatgaa
      181 catcgtgccc ttgctgtcct tgaccttgac cggcgaggtg acgatcacgc ccttgacggt
      241 gaccacggtg tcgaccgcga tcttctcctg ctggatgtcg tagatcgtgg cgtcctcgcc
      301 agcggtctcg gtggtcgggt ccgtggtcgg gtcggtcgag ttggtggtcg gctcggtggt
      361 cggctcggtg gtcggctcgc tggggttgct cgtcggcgtg gtgttgtcgg tggtcggcgt
      421 ggtgtcgctc gtgtcaccgg tcgtggccgt gccgcccgtg ctgctgctgg tcgcagcgtc
      481 gtcggtcgtc gcgtcggtca tcgcgggcgt caggttgcac gcaccggcag cgagggccag
      541 ggcgagggcg gggagggcgg tcaatccacg gtgctgcata agggtgggcg aggagtacca
      601 tcgcgcaccc gcgcgcgaga actaccaccc ggggagagtt ttcgcgggcg gcagcgacgc
      661 ggcaggccgg ccctggggca acggtcggcc gtcgccggag tcgccgggta caaaagcaga
      721 aggccccgct gcgtgtgcag cggggccctc tgagcgcatc cggaaggatt cgaacctccg
      781 accctcggtt ccgtagacca atgctctatc cagctgagct acggatgcgt gttgtttccc
      841 ccgtctccag gggtcgcgga aactatcaag ggcactcggc cattgcaagg tccgccgcgc
      901 tgagagggca tgcctcgagg tgagcgcgaa ctcacgctct tgcggaatct caaggagccg
      961 gcggggtcag caggagcatg gcgagggtca gggcgttgtt gccgaagtga accaccatcg
     1021 cggcaccgat gcgaccggtg cgcgcgtaga cgaggccgaa gacgaggccg agccaggtgt
     1081 agacgacgaa gccctggagg ttgccgtgga aggcggcgaa cagcagggcg gaggcgagat
     1141 aggccgcgac gggcccgccg cgctgccagg tgcggcgcat gagctggccg cgaaagaaca
     1201 gctcctcgcc gaccggggcg agcacgagcg ccgacagggc gagggtcgcc agctcgggcc
     1261 gccgcaggtc gccgcccgcg acgaggtcga ggaccaggcg ctgctcgctg acgggggcgc
     1321 cgagcaggtg catcagcagg gcgaggacgt agctgccggc gatcgccagg aggacgtgga
     1381 ggacgacctg gccggcggtg gcggccggac cggcgacgcg ccagacggcc ggcggcgggg
     1441 cggacaggcc gtcgacgcgg cggccgagcc agccgtaaag cagcgcggcc gcgagggtcg
     1501 ccaggggcgt gagcacgatg ccgtggacct ggcggacctt gtccgggctc agcagcgggc
     1561 gcccgaaggt ggcgtcgagc acccagaaga tcgcgggctg gaccccgggg atcgccagca
     1621 gcagcgcgaa cgcgaggaca aaggcgaaca cggcgagcaa gatctcgctg gtcgcggccg
     1681 agacgcccgc ggccagcccc cccgggcccg caggcccggg accgccggcg ggatcgggct
     1741 cctgtgcctg ctcgggggtc acctgtggac tgtgcaccac gcggcgcccg gggaacaagc
     1801 gcaggccgcc acgctcaccc gtgcccctcg aggcgcttgc aatggcggcc aaacccggat
     1861 aggttgcagc ccccgcaaag caacggggaa ttttttaaaa gggaactcat gatctctcca
     1921 ggaacggtgg ccgagtggct gaaggcacag gtttgctaaa cctgcgtagg gggtaacctc
     1981 taccgagggt tcgaatccct cctgttccgc tgagccgccc ctcgaaactt cgacgggcgg
     2041 ctctcttctt ttaagatcgg tcagcggtag ccggacatgt cgcgcgtgac gccgaggtgc
     2101 agcgtcatac cggcgatctc cgtcagctcg agcaggtcgt cgagcaggcg catctcgttg
     2161 tggtagcgcc ggtcggggtc cttctggtgc aggcgctcga cgatgtcgta gagcagcgag
     2221 ggtgcgtccg ggaccagcga ctcgagcggc ctggggtcct cggagaggat gtggtgcacg
     2281 ccggcgtaga agtcggtgaa gtcgaaggcg ggcacgccgg cgaccatctc gtacatcagg
     2341 ctgccgagcg agaactggtc ggcgcggtag tcgatgcgag cgccgcggaa gctctccggc
     2401 gacaggtaac gcggcgagcc gatgaaggtg cccgggacgg tcagctccga gttggcgagc
     2461 ttggcgatcc cgaagtcgag gatcttggcg acgtcaccct cgaggatgaa gacgttcgcc
     2521 ggcttgacgt cgcggtggat gacctggttg cgatgcgcga actcgagggc gtccgcgagc
     2581 tgaaagccga tgctggcggc gcggcgccac ggcagcttgc cctgcgaggc cagcagggcc
     2641 gccagcgagg tgcccgcgag gtactccatc gccagataca cgggaccctc ggggtcggcg
     2701 gagtagtcgt agacggtgac gatgttgggg tggcgcaaca tgcccgcggc ctgggcctcg
     2761 caggcgaagc gccgcagcag gtcgtcgcgg ccgccgtggt cggagtcgtc ggcgacgacc
     2821 ttgacggcga cggtgcgcgc gagcgccggg tcgaagcact tgaagatggt gcccatgccg
     2881 ccggagccga gctcgctcag cacctgatag cggccgatcg tgcgcggaag ggcttcgtcc
     2941 tcggagctgt tgccgcgcac gcgcgggtcg agcgtcgcgg ccgggtcggc ctgcagcggc
     3001 cgggtgtgat gacccatgac ggtgaccgtc tcgacggcgt cgtgctcgct cgcgcggtcg
     3061 cggcgagcct gcatcacggc gcgcacctcg gtgaccagcg cgtcgagctc gaccggcttg
     3121 acgaggtagt tgatcgcacc gagccgcatg gcatcgacgg cccgcgagat ctggccgtcg
     3181 gcggacagca tcagcaggtc gggcccctgc gtctcacggc ggatcgcctg gagcatcccc
     3241 aggccgtcca tccgcggcat gttgaggtcg gtgatcacca ggtcgaagga cttctgacgc
     3301 acggccgcga gaccctcctg gccatcggcc gcggtgtcga cggtgtagcc gaactcctcg
     3361 aggcaatctc gcaaggtctc ccgcgtgtcc tcgtggtcct cgacgatgag gagcatgccg
     3421 gaatctcgcc cgctgtcggc catcgatgcc tcctcaaccc ccatatcgga ccacggacga
     3481 tccgacaagg ccacgcggtg accggaattc gcccggggcg cggcccgaga cgcggcttgc
     3541 gcggacgcgc ggcggtatga tgcgccgatg cgccttctgc gactgaccgc cccgctgctg
     3601 ttcgcgctca ccgcggcctg gctgctgccc gctccggcga gcgcctgctc ctgcgtcccg
     3661 ccgccaccgc cgagaggcgc gttcgcggag gccaccgccg tgttcgaggg caaggtggcc
     3721 cgcctcgacg aagatcccgc cacacatcgc cgcacggcga cgctcgaggt ggcgcgcgta
     3781 tggaagggcg cagtgaccgc gtccaccagg gtcgccaccc gcgacgccgg agcgatgtgc
     3841 ggcttcgaat tcacggtcgg ccagtcgtac ctgatctacg ccgagggcga ggccgcgagc
     3901 ctgtcgacct ccctgtgctc gcgcagcgcc ccgagcagca aggcaaaggc cgacttcgcg
     3961 gcgctcggcc cgggtaaacc accccctccg ggccagcccg agccggccga gccggtgaag
     4021 ccggtcgagc cggtgaagcc ggtgaagccg gtgaagccgg tgaagccggt cgagccggtg
     4081 aagccagtcg agccggtgaa gccggtcgag ccggtcacgc cggtcgagcc cgcgaagacc
     4141 gcgggctgcg cggtcggtgg ccacgggggt gacgcggggg ggctcgcggt gctggtgttg
     4201 ttcgcggggc ggcggcgacg gcctggctga gcgcggatca ggccgcgacg agctgccggc
     4261 gcaggtgggc tcgcgcgcgc gacaggcgga ccgagacgtt gcgctcggag atccccaggg
     4321 tctcggcgat ctgcgcgtag gaccgcccgg tcaggtggag ggtgagcacg gactcgaggg
     4381 tgtcgggcag atcggtcagc gcctgctcga tgcgggcgcg ctggtcggcg cgcaggatcg
     4441 ccgactcgat gcaggcgccg gggtcggcga cggtcgcagg gtcggtggcg tcctcgaggt
     4501 ccttgcggcg gcggacgtgg cgaaaacagc agtagcgggc gacccggtag gcaaaggtct
     4561 tgagcgagga ctcgccgcgg aaggtcggca gcgcctgcca cagggcgagc gcgatgtcct
     4621 gctcgaggtc ctcgcgctcg gccgcgccgc gcgtgtaact gccggccatc cgggagatcc
     4681 cgcggccgtg gtcggcgagc gtgccggccc agcgcgcgcg atgagcccgg tcgtccgccc
     4741 gctcgctgtc gtcgcccggt cgcgcggccg gggcgtcgtg gatcggtatg accgcggcgt
     4801 gctcgcctcc gctggcccgg taacccgagg ggctggcacc cgcgagccg
//
LOCUS       DASWNJ010001118         4030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_125301, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001118 DASWNJ010000000
VERSION     DASWNJ010001118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125301"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..1881)
                     /locus_tag="VGB85_18850"
     CDS             complement(202..1881)
                     /locus_tag="VGB85_18850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136155.1"
                     /translation="MASTLLEPIAAGQTRFHMQGVAPDGRGIAVGRETLLVFPSLDRL
                     VAFLGAYSEECSLDDLIPSLTLERARREGGGQALLLRCAGGDSYALDRLGRMATAVHG
                     QLFTGSGTVFVRYRDRFAPFGYDLAGPAGRAPAPRPPAPRPPGTAPAGAVLLAEEVLV
                     VDHDYQARYRATDRLDPVELIQRLQLRAVPLPPGGLADSPDLCGLHDLALALVAPGLS
                     DRVLAYLWGNQIAMAGVRVALAGERRPSLLLRLRQPSGQVLSVLHGIPGVELLVPVSP
                     RAAVELGYRHPIHLASASSCLPGDEMYLFRGRVARVERLEGAPQFIDGSHLVVSQTRV
                     ASPGAVGQPGDARGVPSTSLQVALRLRPSAGAREPRGALVRWEQAGLLRKLVYLIPPT
                     ALAAARLAVLPQGMLVLTGSSLGGAGAGLAAGVGAAALIPLGTRIVEVAPGVLVPDGF
                     ELWPHVRPQLVRELLGLEAADHALFLGPDTDPLRLRPDHLIPLDAAMISRLELAVSEA
                     PPAELLTRPEGAAASVVNEHLGRFALWGFGRVDNAPIPATAGRELPSGRDD"
     gene            2252..3469
                     /locus_tag="VGB85_18855"
     CDS             2252..3469
                     /locus_tag="VGB85_18855"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0136156.1"
                     /translation="MVGELGRARALEHAGDHAQAAALRLEYAHTLCGTVQRIAMLREG
                     CARNSGATEEGRALHQALAETLLRHAEFMEDGAPRRAILMEAARALEEADQGGVAGEI
                     YERLGMLRRAARAFERAGAISQLEYVLGLLDRHEQIDQALQNALQAVDAALREGSRLL
                     AHSLLQEHLYQQRSGFAAATPPAIGPGAAARGGLVQRLANLEAALPRGPRLGLRWQGH
                     VTQVLLAPQLRIGRAPDVELSLPGAGLSREHVVLRIEAGARGLAIAAVDLGSKAGSFW
                     RGEALEPGEPQVLDEPGELGLGFGNGVHVHPLRESSESQGSFGALLKAQGASDWLLFL
                     PGGGPLWLDPGHALPARIDLRPPFVGLQVAAGVAAHLGSEPLGPGASLELMLGDRLHL
                     TLPDGRLTLEVIA"
     gene            3495..>4030
                     /locus_tag="VGB85_18860"
     CDS             3495..>4030
                     /locus_tag="VGB85_18860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136157.1"
                     /translation="MSVIDPALEQALRAVLARLSEGAALPREHAGVASLTRLLAENAP
                     LRIVEGQVRELSRGGYLRGALVSVAGHQRLRPRPDLLLLLATELCDALAPDLGVELAR
                     AVLALPGVQAARWSPGGPYVAANLLLGEALAAAGEGLAALRHFEAVLAVDVDHARALR
                     GYHEAVRRGAGGPAPSRGL"
BASE COUNT          510 a         1479 c         1608 g          433 t
ORIGIN      
        1 cgacacccag cagccgcgct tcccatctct cgaccgcgac acctggccca cgcagccgag
       61 ccccgcgttg ctcgaacgcg acactcccac acagccgagt cccgcgaacg accgcgacac
      121 tcggcccatg cagccgagcc ccgcgagaga gcccgacatg cctgacatcg gatccaagct
      181 atcggccccc cgatccatcg gtcagtcgtc ccggcccgaa gggagctcgc ggccggcggt
      241 ggccgggatc ggggcgttgt cgacgcgacc gaagccccac agggcgaagc ggccgaggtg
      301 ttcgttgacg acggacgcgg cggcgccctc ggggcgggtg agcagctcgg cgggtggggc
      361 ctcgctgacg gcgagctcga ggcggctgat catcgcggcg tcgagcggga tcaggtggtc
      421 ggggcgcagg cgcagcgggt cggtgtcggg gccgaggaac agggcgtggt cggcggcctc
      481 gaggccgagc agctcgcgga ccagctgcgg gcggacgtgc ggccacagct cgaagccgtc
      541 gggcacgagc acgccggggg cgacctcgac gatgcgggtg ccgagcggga tcagggccgc
      601 cgcgccgacg ccggcggcga ggcccgcccc ggcgccgccg aggctcgagc cggtcagcac
      661 gagcatgccc tgcggcagca cggcgaggcg ggccgccgcg agggccgtcg gtgggatcag
      721 gtagacgagc ttgcgcagca gcccggcctg ctcccagcgc acgagggcgc cgcgcggctc
      781 gcgggcgccg gccgaggggc gcaggcgcag ggcgacctgc agcgaggtgc tcggcacgcc
      841 gcgggcgtcg ccgggctggc cgacggcgcc gggcgaggcg acgcgggtct gcgagacgac
      901 gaggtggctg ccgtcgatga attgcggcgc gccctcgagg cgctcgacgc gggcgacgcg
      961 gccgcggaac aggtacatct cgtcgccggg caggcacgac gaggcggacg cgaggtggat
     1021 cgggtggcgg tagccgagct cgaccgcggc gcgcggcgag accgggacga gcagctcgac
     1081 gccggggatc ccgtgcagca ccgacagcac ctggccgctc ggctggcgca ggcgcagcag
     1141 caggctcgga cggcgctcgc cggcgagggc gacgcgcacg ccggccatcg cgatctggtt
     1201 accccacagg taggcgagga cgcggtccga caggcccggg gcgacgagcg cgagcgcgag
     1261 gtcgtggagg ccgcacaggt cgggcgagtc ggcgaggccg ccgggcggca gcggcacggc
     1321 gcgcagctgc aggcgctgga tcagctcgac ggggtcgagg cggtcggtcg cgcggtaacg
     1381 ggcctggtag tcgtggtcga cgaccagcac ctcctcggcg agcagcacgg cgcccgcggg
     1441 cgcggtgccc gggggtcgtg gggccggggg tcgtggggcc ggggcgcggc cggccgggcc
     1501 cgcgaggtcg tagccgaagg gggcgaagcg gtcgcggtag cggacgaaca cggtgccgct
     1561 gccggtgaac agctggccgt gcaccgcggt ggccatgcga ccgaggcggt cgagcgcgta
     1621 gctgtcgccg ccggcacagc gaagcagcag cgcctgtccc ccaccctcgc ggcgggcgcg
     1681 ctcgagcgtg agcgagggga tcaggtcgtc gaggctgcac tcctcgctgt aggcgccgag
     1741 gaacgcgacc aggcgatcga gcgacgggaa caccagcagt gtctcgcggc cgaccgcgat
     1801 cccgcggccg tcgggggcca caccctgcat gtgaaagcgc gtctggccgg cggcgatcgg
     1861 ctcgagcagg gtggacgcca tggcccgcag tgtacccgcg gaccgtgcta gcatccacag
     1921 ccccgtggcc gcccccaaca agccgcagcg cccccctgcg ccgccgatga cctccgggcc
     1981 cgcgggatcc tccggaagct ccgggtcctc cgggtcctcc ggaccgcgag atccatcgca
     2041 gggccgccgc gagcccgacc cgggggaacg accgcagcgg ccgccggacg cccaggtccc
     2101 gcgcggatcg ggcgccgggc gcctgccgat gcgcagcggc gatccccgga gcggcgggcg
     2161 accgcccggg gtgaccaccg cgtcccccac cgcgtcccct caccatggcc cttcggacag
     2221 gtcgcaaggg acaggtcccc ggggacagca ggtggtcggt gagctcggcc gcgcccgcgc
     2281 gctcgagcac gccggcgacc acgcgcaggc ggccgcgctg cgcctcgagt acgcgcacac
     2341 gctgtgcggc acggtgcagc ggatcgcgat gctgcgcgag ggctgcgcgc gcaactcggg
     2401 cgcgaccgag gagggccgcg cgctgcacca ggcgctcgcc gagacgctgc tgcgccacgc
     2461 cgaattcatg gaggacgggg cgccacgccg cgcgatcctc atggaggccg cgcgcgcgct
     2521 cgaggaggcc gaccagggcg gcgtcgccgg cgagatctac gagcgcctgg gcatgctgcg
     2581 ccgggccgcc cgcgccttcg agcgcgccgg ggcgatcagc cagctcgagt acgtgctcgg
     2641 cctgctcgac cgccacgagc agatcgatca ggcgctgcaa aacgccctgc aggccgtcga
     2701 cgccgcgctg cgcgagggca gcaggctgct cgcccactcg ctcttgcaag agcacctgta
     2761 tcagcaacgc agcggcttcg cggccgcgac cccgcccgcg atcggtcccg gcgcggcggc
     2821 ccgcggcggg ctcgtgcagc ggctcgccaa cctcgaggcc gccctgcccc gcggtccccg
     2881 gctcgggttg cgctggcagg gtcacgtgac ccaggtcctg ctcgcgccgc agctgcggat
     2941 cggtcgcgcg ccggacgtcg agctctcgct gccgggggcc gggctgtcgc gcgagcacgt
     3001 ggtgctgcgg atcgaggccg gcgcgcgggg gctggcgatc gcggcggtcg acctcggctc
     3061 gaaggcgggc agcttctggc gcggcgaggc gctcgagccc ggcgagccgc aggtgctcga
     3121 cgagcccggc gagctcggcc tcggcttcgg caacggcgtg cacgtgcacc cgctgcgcga
     3181 gagcagcgag tcgcaaggaa gcttcggcgc cttacttaaa gcccagggcg cgagcgactg
     3241 gctgctgttc ctgcccggcg gcggtccgct gtggctcgac cccgggcacg ccctgcccgc
     3301 ccggatcgac ctgcgcccgc ccttcgtcgg cctgcaggtc gcggccggcg tcgcggcgca
     3361 cctcggcagc gagccgctcg gtccgggcgc gagcctcgag ctgatgctcg gcgaccgcct
     3421 gcacctcacg ctgccggacg gccgcctgac gctcgaggtg atcgcgtgag catgtccgaa
     3481 gggacaggga tcggatgtcg gtgatcgacc cggcgctcga gcaggcgctg cgggccgtgc
     3541 tggcgcgcct gtccgagggg gcagctttgc cgcgcgagca cgcgggcgtc gcctcgctga
     3601 cgcgcctgct cgccgagaac gcgccgctgc ggatcgtcga gggccaggtg cgcgagctca
     3661 gccgcggcgg ctacctgcgc ggcgcgctcg tcagcgtggc cgggcaccag cgcctgcgcc
     3721 cgcgcccgga cctgctgctg ctgctggcga ccgagctgtg cgacgcgctc gcgccggacc
     3781 tcggcgtcga gctcgcgcgc gccgtgctgg cgctgccggg cgtgcaggcc gcccgctgga
     3841 gcccgggtgg gccctacgtc gccgccaatc tcctgctcgg cgaggcgctc gcggccgcgg
     3901 gcgaggggct cgcggcgctg cgtcacttcg aggcggtgct cgcggtcgac gtcgaccacg
     3961 cgcgcgcgct gcggggctac cacgaggcgg tgcgccgcgg cgcgggtggg ccggcgccga
     4021 gccgcgggct
//
LOCUS       DASWNJ010001119         1711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_656696, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001119 DASWNJ010000000
VERSION     DASWNJ010001119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656696"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /gene="pyrE"
                     /locus_tag="VGB85_18865"
     CDS             <1..649
                     /gene="pyrE"
                     /locus_tag="VGB85_18865"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEY0136158.1"
                     /translation="IELLVRHEVLRFGDFTLKSGRRSPYFVNAGQFRTGAAISGLGRA
                     YAERLHRAGLRCDLLFGPSYKGVPLAVATAIAMAELGHDAPFCFDRKEEKSHGEGGWF
                     VGTAPQGGMRVVIVDDVITSGKSIHESVELLRRTADVQIAGVVVAVDRQERGTGERTT
                     LAELQSELGVPVCPIVTIRDVIAHLHGRDVDGVQVIDDERRAAMEAHLAAFGGTA"
     gene            complement(654..1055)
                     /locus_tag="VGB85_18870"
     CDS             complement(654..1055)
                     /locus_tag="VGB85_18870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136159.1"
                     /translation="MDETLQRTQTQLHASLAFHDLPDERRRKLMTDVAAVAAFLNPPP
                     TPPRAGKGRPGVDRRMSTRPDMLPEYVSSLVMGTFQPVVEASVHQMAAYVELMASVAG
                     SIEGLWADAEQRTLLAHRLEVELRKIFRDGD"
     gene            1405..>1711
                     /locus_tag="VGB85_18875"
     CDS             1405..>1711
                     /locus_tag="VGB85_18875"
                     /inference="COORDINATES: protein motif:HMM:NF019255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1587 domain-containing protein"
                     /protein_id="HEY0136160.1"
                     /translation="MGMGHGMGVRAGLLALALTACGDAGGAEATATAGASEATSGDGT
                     GDEPTSGEPAGPCAGIEPGDQVIRRLNRSEFRNTVRALLGHDGDPAAELAPDPRSFGF
                     "
BASE COUNT          229 a          620 c          606 g          256 t
ORIGIN      
        1 catcgagctg ctcgtccgtc acgaggtgtt gcgcttcggc gacttcacgc tcaagtccgg
       61 ccggcgctcg ccctacttcg tcaacgccgg ccagtttcgc accggcgctg cgatctcggg
      121 cctcggtcgc gcgtacgccg agcgcctgca tcgcgccggg ctgcgctgcg acctgctgtt
      181 cggcccgtct tataaaggcg tgccgctggc cgtcgccact gcgatcgcca tggccgagct
      241 cgggcacgat gcgccgtttt gcttcgaccg caaggaggag aagtcccacg gcgagggcgg
      301 ctggttcgtc ggcaccgcgc cgcagggcgg catgcgcgtc gtcatcgtcg acgatgtcat
      361 cacctccggc aagtcgatcc acgagagcgt cgagctgctg cgccgcaccg ccgacgtgca
      421 gatcgccggc gtggtcgtcg cggtcgaccg ccaggagcgc ggcaccggcg agcgtacgac
      481 cctggccgag ctgcaaagcg agctcggcgt ccccgtgtgc ccgatcgtca cgatccgcga
      541 cgtcatcgcg cacctgcacg gccgtgatgt cgacggcgtg caggtgatcg acgacgagcg
      601 ccgcgcggcg atggaggccc acctcgcggc cttcggcggg accgcctgac cgctcagtcg
      661 ccgtcgcgga agatcttgcg cagctcgacc tcgagccggt gggcgagcag cgtgcgctgc
      721 tcggcgtcgg cccacaggcc ttcgatcgac ccggcgacgc tcgccatcag ctcgacgtag
      781 gcggccatct ggtgcaccga ggcctcgacg accggctgga acgtgcccat caccagcgag
      841 ctgacgtact ccggcaacat gtcggggcgc gtcgacatcc tgcggtcgac cccgggccgg
      901 cccttgcccg cgcgcggagg cgtcggcggc gggttgagga acgccgcgac cgcggcgaca
      961 tcggtcatca gcttgcgccg ccgctcgtcc ggcaggtcgt gaaaggccag cgaggcgtgc
     1021 agctgggttt gagtgcgctg gagggtctca tccatgatcg tgggtcctga tggcccgggc
     1081 tgcgggcgcc cgcgtggcga tgcgcgagta tatcccagcc gcccggcgcc cccgggctcg
     1141 gccgacccca accctgtgac gtcggacacc gcgggcctgt ccgtcaggac agccgggcca
     1201 gccccttcga ccacatgccc gcatacctgc gccgcgagtg cgcctccgcg cctgcggtcc
     1261 tctacccccg tcctcgtggc cgctccatcg ctcgcgcgac agcgccgtga tgatctgtct
     1321 catgagacag gtaacatcgg tgtaggccga cgaccccgcg tagccgcttg acagccgctg
     1381 tgcgccgcgc gtatcgagga tgagatgggg atggggcatg gcatgggtgt gcgtgcgggc
     1441 ctgctcgcgc tcgcgttgac ggcctgcggc gacgccggtg gagccgaggc gaccgcgacc
     1501 gcgggcgcca gcgaggcgac gagcggggac ggtaccggcg acgagccgac ctcgggcgag
     1561 ccggcaggtc cgtgcgccgg catcgagccc ggcgaccagg tgatccgccg cctcaatcgc
     1621 agcgagtttc gtaacaccgt gcgcgcgctg ctcggccatg acggggaccc cgccgccgag
     1681 ctcgcgcccg acccgcgcag cttcggtttt g
//
LOCUS       DASWNJ010001120         3099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_402838, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001120 DASWNJ010000000
VERSION     DASWNJ010001120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402838"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="VGB85_18880"
     CDS             <1..1197
                     /locus_tag="VGB85_18880"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEY0136161.1"
                     /translation="SIFIQTYTGEYPEEDVTDPVDRDVILRPDPNSIRLVPWYEEPTA
                     QVIADCTYHDGKPVEISSRGVLRRILKLYEARGWRPVVAPELEFFLTKVNEDPDYPLV
                     PPIGRSRRQETARQSFGIDAVNEFDPIFEDVYDWCEAQEIDIDTLSHEAGAAQMEINF
                     NHGDALELADQAFLFKRTLRQAALKHGIYATFMAKPMEAEPGSSMHIHLSLYDVEGGH
                     NLFAAPDGGGGHTPLFLNFIGGLQEYLPAAMPLMAPNVNSYRRLRRHSTAPINVHWGL
                     ENRTVGLRVPASRPEACRVENRLAGADANPYLAIAASLACGWLGITRAIEPTDPIKGS
                     AYRLAQTLPQQMPEAMRKLKHATVLKECLGEDFVEVLLAVKAAEHDAYQRVISSWERE
                     HLLLNV"
     gene            1290..1811
                     /locus_tag="VGB85_18885"
     CDS             1290..1811
                     /locus_tag="VGB85_18885"
                     /inference="COORDINATES: protein motif:HMM:NF013853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 domain-containing protein"
                     /protein_id="HEY0136162.1"
                     /translation="MGKQLAREPYDDAAISYEADPYAWALVQADLLRAGRFDDLDLAN
                     LIDEVADLARAQRSAVLNNARIVVEHLLKLAHSPAGEPRNGWRATVREHRQRLALDLT
                     RTLRRELGEALPRVFQLARSSAEGALRDHGEDGAAVALPSTCPFDLDHITGDWWPDDP
                     REPRPAPAEQALA"
     gene            complement(1808..2227)
                     /locus_tag="VGB85_18890"
     CDS             complement(1808..2227)
                     /locus_tag="VGB85_18890"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEY0136163.1"
                     /translation="MLSTLAFVTSAALLGGMLYFALGFAPLVFTRLPAETAARFIRAV
                     FPVYYAMGAGTALLATLLLLPTGRWAAIALAAAVAGGFLLARFALMPAVNAARDRGMA
                     GDDAANRRFAKLHGGSVALNGAQALALVGILVLLAKG"
     gene            complement(2352..2843)
                     /locus_tag="VGB85_18895"
     CDS             complement(2352..2843)
                     /locus_tag="VGB85_18895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136164.1"
                     /translation="MGSGSRSIRSLAAEAGVPVEECLDRLRAAGIKAGVGAQKLDGQA
                     LASAQAALGVRARLAEPTPTAGRRDLEGDELLVRLLRPLREKGKVGRDHTTSVEHVWG
                     HGLPDHQKAEAKRLVEALLTEGCLDEKVSQGRRQLWLTNSGLERLSRAEAVAAASGAP
                     SAS"
     gene            complement(2867..>3099)
                     /locus_tag="VGB85_18900"
     CDS             complement(2867..>3099)
                     /locus_tag="VGB85_18900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136165.1"
                     /translation="CGDHGQFSAWGRMSESDAPQPCPSCREPAGRTLARPAIGGRSGA
                     GDDMGCGEGGCAAGESPMMAGGACCGGGACVH"
BASE COUNT          456 a         1139 c         1096 g          408 t
ORIGIN      
        1 agcatcttca tccagaccta caccggcgag tacccggagg aggacgtcac cgacccggtc
       61 gaccgggacg tgatcctccg ccccgacccg aactcgatcc ggctcgtacc ttggtacgag
      121 gagccgacgg cgcaggtgat cgccgactgc acctaccacg acggcaagcc ggtcgagatc
      181 tcgtcgcgcg gcgtgctgcg gcggatactc aagctctacg aggcgcgcgg ctggcggccg
      241 gtggtggcgc ccgagctcga gttcttcctg accaaggtga acgaggaccc ggactacccg
      301 ctggtgccgc cgatcggccg ctcgcgccgg caggagaccg cgcgccagtc gttcgggatc
      361 gacgcggtca acgagttcga cccgatcttc gaggacgtct acgactggtg cgaggcgcag
      421 gagatcgaca tcgacacgct ctcccacgag gcgggcgcgg cgcagatgga gatcaacttc
      481 aaccacggcg acgcgctgga gctggccgac caagccttcc tgttcaagcg cacgctccgc
      541 caagcggcgc tcaagcacgg catctacgcg accttcatgg ccaagcccat ggaggccgag
      601 cccggcagct cgatgcacat ccacctcagc ctctacgacg tcgagggcgg gcacaacctg
      661 ttcgcggccc cggacggcgg cggcggccac acgcccctgt tcctcaactt catcggcggc
      721 ctccaggagt acctgcccgc cgccatgccg ctgatggcgc ccaacgtgaa ctcctaccgc
      781 cggttgcggc ggcactcgac ggcgcccatc aacgtccatt ggggcctgga gaaccgcacc
      841 gtcggcctgc gcgtcccggc ctcgcgcccc gaggcctgcc gggtcgagaa ccgcctggcc
      901 ggcgccgacg ccaaccccta cctcgcgatc gcggcctcgc tcgcctgcgg ctggctcggc
      961 atcacccgcg cgatcgagcc caccgacccg atcaagggca gcgcctaccg tctggcccag
     1021 accctgccgc agcagatgcc cgaggcgatg cgcaagctga agcacgcgac ggtgctcaag
     1081 gagtgcctgg gcgaggactt cgtcgaggtg ctgctcgcgg tgaaggcggc cgagcacgac
     1141 gcctaccagc gggtgatcag ctcgtgggag cgggagcacc tgctgctcaa cgtgtgaagg
     1201 ttacggacgc gagtgctgtc gaggtcttcg ggcgatagcc gttgcgcttg gcgaagctaa
     1261 gggcggatac cgcctcgcgg agggcaccaa tgggtaagca gcttgcccgc gagccgtacg
     1321 acgacgccgc gatctcctac gaggcggacc cttacgcctg ggcgctcgtg caggccgacc
     1381 ttctccgcgc tggccggttc gacgacctcg acctcgccaa cctgatcgac gaggtggccg
     1441 acttggctcg ggcgcagcgc agcgcggtcc tcaacaacgc caggatcgtc gtcgagcacc
     1501 tgctgaagct cgcgcacagc ccggccggcg agccgcgcaa cggctggcgc gcgacggtgc
     1561 gggaacaccg ccagcgcctc gcgctcgacc tgaccaggac cttgcgccgg gaactcggcg
     1621 aggcgctccc gcgagtcttc cagctggccc gctcgtcggc cgagggtgcg ctgcgcgacc
     1681 acggtgagga tggcgcggcg gtcgccttgc cgtcgacctg cccgttcgac ctggaccaca
     1741 tcaccggcga ctggtggccg gacgacccgc gcgagccacg accggcgccg gccgagcaag
     1801 ccctcgccta gcctttcgcg agcagcacca ggatcccgac cagcgccagc gcctgcgccc
     1861 cgttcagcgc gacgctgccg ccgtggagct tggcgaagcg gcggttcgcg gcgtcgtcgc
     1921 ccgccatccc tcggtcgcgt gcggcgttga ccgccggcat cagggcgaag cgggcgagga
     1981 ggaagcctcc ggccaccgcg gcggcgaggg cgatcgccgc ccagcgcccg gtcggcagca
     2041 ggaggagcgt ggcgaggaga gccgtgccgg cgcccatcgc gtagtagacc gggaacacgg
     2101 cccggatgaa ccgcgccgcc gtctcggccg gtagccgcgt gaagacgagc ggggcgaagc
     2161 cgagcgcgaa gtagagcatg ccacccagga gcgcggcact cgtgacgaag gcgagggtac
     2221 tcagcacgag gcgatagtct ccttgtggag ggccggcacg cgggccgctg ggcgtcaagt
     2281 tgtggtcttc ggcgggtgcc gccacggggc cgagcgtcgc ggtctcgcgg gaggcggacg
     2341 ctccggcgag gtcagcttgc cgacggtgcg cccgatgccg ccgcgaccgc ctcggcgcgg
     2401 gataggcgct cgaggccgct gttggtgagc cagagttgtc gccgcccctg gctgaccttc
     2461 tcgtcgaggc agccctcggt cagcagcgcc tcgaccagcc gcttggcctc ggccttctgg
     2521 tggtcgggca ggccgtggcc ccagacgtgc tcgaccgagg tggtgtggtc gcggccgacc
     2581 ttgcccttct cgcgcaacgg ccgcagcaaa cgcacgagca gctcgtcgcc ctcaaggtcg
     2641 cggcggccgg cggtcggcgt cggttcggct aggcgtgccc ggacgcccag ggcggcctgc
     2701 gcgctcgcca gggcttggcc gtcgagcttc tgcgcgccga ccccggcctt gatgccggcg
     2761 gcgcgcagcc ggtccaggca ctcctcgacc gggacgcccg cctcggcggc gaggctgcgg
     2821 atcgaccggc tgccgctacc cacgcgaccg aagccttcca gtcgtgtcag tgcacgcagg
     2881 ccccgccgcc gcagcacgcg ccgccggcca tcatcgggga ctcgcctgcc gcgcacccac
     2941 cctcgccgca gcccatgtcg tcgcccgcac ccgagcgtcc gccgatggcg ggacgggcca
     3001 gggtgcggcc tgccggctcg cggcaggagg ggcagggctg cggggcgtcg ctctcgctca
     3061 tccggcccca ggcggagaac tggccgtggt cgccgcagg
//
LOCUS       DASWNJ010001121         4636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_159791, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001121 DASWNJ010000000
VERSION     DASWNJ010001121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159791"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4603)
                     /locus_tag="VGB85_18905"
     CDS             complement(<1..4603)
                     /locus_tag="VGB85_18905"
                     /inference="COORDINATES: protein motif:HMM:NF023677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin TcdB middle/N-terminal domain-containing
                     protein"
                     /protein_id="HEY0136166.1"
                     /translation="MTGAPALDPTADGRAWVDVDGDALPDLLDGEPGAWRYRRNIGET
                     RLAATWTDLPSPAVSLTPTTRFADLTGDGVQDLLAQPTPGELWSFVGGGATPFGAASP
                     ITLDLSFDLTDPRVALADMNLDGRVDVLRHDDADGWCWLRLRDTAGYEPAEVVPPPPA
                     GMRLGEPGVQLADMNGDRIPDLVHVITEDNRILVAAGAGLGLFEDPADMVGVPPMMQT
                     DRWEIADINGDGAADLLRIGQVQLGLWINELDGSFAEAGGVAWPALEADEVVLVTDID
                     GSGTLDILRADTDGSQPWRAWSVFSERPGLLSRFENGLGYSREHTYRSAAQLAAEDAA
                     AGTPWTTAPPEAMPVLTETREDDGAGWSSTLRRRLRDGWYDPARGEFRGFAELRDETT
                     GDAYTEAATITRSYDLGQQDEARALQLLAAETRSPRGVLVREAHTLAVDTPAAGVRAV
                     HRTASDTYHLEAGPEAAAVRVRVEWDHDAWANVLEERAFGRVDRQTGADLPGDERITT
                     YSYATASSDDGPRDRIAEQIVMDADGAQITATRTYYDGEPEQGLPLGQLAARGVVART
                     QTWIAGNTWDPTLRQAVDPHGNVTHVRDAEDGTLERRYDAAGLFPVEERLLLPAGALV
                     TTAEWDARFGHPLAVKAPSGATTRATYDGLGRLVAEILPGDTVELPTTRHVYYLDGTT
                     ARPTIVTELRRISGEPDVDIVAAHLDGLGRPRLRVTQDDTGTAAILAETRIYSDAGAV
                     AELVEGQPLAAGALAPGANVEIAGTWPRSVTHADALGRIVFTRDADGRESATTYGPLW
                     TERRAHEDLHPTPPYQDTPERSEQDGLGHTLARKQLLADRTIVHAYSHDAAGRLVATI
                     DPAGHKTTFTRDGAGRLTQIDSPDAGALRQRFDRTGRITECINATGARVTWAFDPLGR
                     LLHERAYSSTGAQVGGVRHYYDGSGPFARGQLTRVDDDAGSVAFTYDARSRIVTSTRT
                     FAAADGPVTLTAGQVYDAQDRILRDIFPDGSTLEHDYSPRGLERPLESFIERADYDAL
                     ARWRELELPSGVTLRRDLDHGGRVLGQRVTAGSRGLLDLAHRYDVAGQLAETHDDLAA
                     EGFSLSQTFVYDDLRRLIGHTAAGKTQTWRYSDDGNLLAHAGRTLGYDDDRPHAAVAL
                     DRQALEYDTAGQLATVTGDGPLPAGAWHFDPQGRVQSFTAADGRRVEHVHGYTGERAI
                     RREYDAAGQLAREVLYFTRNLEIRDGKLVRWVYFAGERIAESPVPLPAGGFPELPAET
                     LTVTPPVDPRHLLGLLLLAFGALALRVLAMRPRRLLVAATLALVCSAATLSCHHNPDH
                     TLTPDEHTRFHVDDRLGSAALVLDHRGSVLTRDAADPYGAPRLAWRADEDTAAPTYRF
                     TGKEDDPLSGAIAIGARHYLPELGRWASPDPHFLLENPDAALATPGEANPYSYVGGNP
                     VTFTDPTGRKGLAHGSENRPGVLDNFPDSPNSGFDSRMAAVAHDRALAEYRGAQLASM
                     AYGVLDVALTGLDWAMTASDVVDVASLAAGPPGLGYKAGK"
BASE COUNT          577 a         1479 c         1905 g          675 t
ORIGIN      
        1 gcttgccagc cttgtagccc aggccgggcg ggccagcggc gaggctggcc acgtcgacca
       61 catcggaggc ggtcatcgcc cagtcgaggc cggtcagtgc cacgtcgagg acgccgtagg
      121 ccatgctggc gagctgggcg ccgcggtact cggcgagggc ccggtcgtga gccacggccg
      181 ccatgcggct gtcgaagccg gaattcgggc tgtcggggaa gttgtcgagg acgccgggac
      241 ggttctcgct gccgtgcgcg aggcccttgc ggccggtcgg gtcggtgaac gtgacggggt
      301 tgccgccgac gtagctgtag gggttggcct cgccgggggt ggctagggcg gcgtccgggt
      361 tctcgagtag gaagtgcggg tcggggctcg cccagcggcc gagctcgggg aggtagtggc
      421 gggcgccgat ggcgatggcg ccgctcaggg ggtcgtcttc cttgccggtg aaacggtagg
      481 tcggtgcggc ggtgtcctcg tcggcgcgcc aggcgaggcg gggggcgccg taggggtcgg
      541 cggcgtcgcg ggtgagcacg ctgccgcggt gatcgaggac gagcgccgcg gagccgaggc
      601 gatcgtcgac gtgaaagcgg gtgtgctcgt cgggggtgag cgtatggtcg gggttgtggt
      661 ggcagctcag ggtggcggcg gagcagacga gcgcgagcgt ggcggcaacg aggagccggc
      721 gcggccgcat ggcgagcacg cgcaaggcca gcgcgccgaa cgcgagcaac aggaggccga
      781 gcaggtgccg cggatccacg ggcggagtga ccgtcagcgt ctcggcgggc aactcgggga
      841 agccgcccgc ggggagcggg acggggctct cggcgatgcg ctcgcccgcg aagtagaccc
      901 agcgcacgag tttgccgtcg cggatctcga ggttgcgggt gaagtagagg acctcgcgcg
      961 cgagctggcc ggcggcgtcg tactcgcggc ggatcgcccg ctcgccggtg tagccgtgca
     1021 cgtgctcgac gcggcggccg tccgccgcgg tgaacgactg tacacggccc tgcgggtcga
     1081 agtgccaggc gcctgcggga agcgggccgt cgccggtgac cgtggcgagc tggccggcgg
     1141 tgtcgtactc gagcgcctgg cggtcgaggg cgacggcagc gtgggggcga tcgtcgtcgt
     1201 agccgagggt gcggcccgcg tgcgcgagca gattgccgtc gtcgctgtag cgccaggtct
     1261 gcgtcttgcc cgcggcggtg tggccgatca ggcgccggag gtcgtcgtag acgaaggttt
     1321 gcgacagcga gaagccctcc gcggccaggt cgtcgtgagt ctcggcgagc tggccggcga
     1381 cgtcgtagcg gtgcgcgagg tcgaggagcc cgcgtgagcc cgcggtgacg cgctggccga
     1441 gcacgcggcc gccgtggtcg aggtcgcggc gcagcgtcac gccggagggc agctccagct
     1501 cgcgccagcg ggcgagcgcg tcgtagtcgg cgcgctcgat gaaggactcg agcggccgct
     1561 cgaggccgcg gggtgagtag tcgtgctcga gcgtgctgcc gtccgggaaa atgtcgcgca
     1621 ggatgcggtc ctgcgcgtca tagacctggc ccgcggtcag cgtgaccggg ccatccgcgg
     1681 cggcgaaggt gcgcgtggac gtgacgatgc ggctgcgggc gtcgtaggtg aacgcgacgc
     1741 taccggcgtc atcatcgacg cgcgtgagct ggccgcgagc gaacgggccg ctgccgtcgt
     1801 agtagtggcg gaccccgccg acctgcgcgc ccgtggagct gtaggcacgc tcgtgcagga
     1861 ggcggccgag cgggtcgaag gcccaggtga cgcgcgcgcc ggtcgcgttg atgcactcgg
     1921 tgatgcggcc cgttcgatcg aagcgctggc gcagcgcgcc ggcgtcgggt gagtcgatct
     1981 gcgtgaggcg accggcgccg tcgcgggtga aggtcgtttt atggccggcg gggtcgatgg
     2041 tggcgacgag gcggcccgcg gcgtcgtgac tgtaggcatg gacgatcgtg cggtcggcga
     2101 ggagctgctt gcgcgcgagc gtgtggccga ggccgtcttg ctcgctgcgc tcgggggtgt
     2161 cctggtacgg gggcgtgggg tggagatcct cgtgagcgcg gcgctcggtc cacagggggc
     2221 cgtaggtcgt ggcgctctcg cggccgtccg cgtcgcgcgt gaagacgatg cggcccagcg
     2281 cgtcggcatg cgtcacgctg cggggccagg tgcccgcgat ctcgacgttg gcgccgggcg
     2341 ccagggcgcc ggcggcgagc ggctggcctt ccacgagttc ggcgacggcg cccgcatcgc
     2401 tgtagatgcg ggtttccgcg aggatcgcgg cggtgccggt gtcgtcttgc gtgacgcgaa
     2461 ggcgcgggcg gccgaggccg tccaggtggg cggcgacaat atcgacgtcc ggctcgccgc
     2521 tgatgcggcg cagctccgtg acgatcgtcg ggcgcgccgt ggtgccgtcc aggtaataaa
     2581 catggcgcgt ggtcggcagc tccacggtgt caccggggag gatctcggcg accaggcggc
     2641 cgaggccgtc gtaagtcgct cgcgtcgtgg cgccgctcgg ggccttgacc gcgagcggat
     2701 ggccgaaccg tgcgtcccac tcggctgtgg taaccagcgc gccggcgggg agtagcaggc
     2761 gttcctcgac ggggaacagg ccggcggcat cgtagcggcg ctcgagcgtg ccgtcctccg
     2821 cgtcgcggac atgcgtgacg ttgccgtggg ggtcgacagc ctgtcgcagc gtggggtccc
     2881 atgtgttgcc cgcgatccag gtctgggtac gagcgacgac gccgcgggcc gcgagctggc
     2941 cgagcgggag gccctgttcg ggctcgccgt cgtagtaggt gcgcgtggca gtgatctggg
     3001 cgccgtccgc gtccatcacg atctgctcgg cgatgcggtc gcgcgggccg tcgtcgctgc
     3061 ttgctgtcgc gtagctgtag gttgtgatcc gctcgtcgcc ggggaggtcg gcgccggtct
     3121 ggcggtcgac gcggccgaag gcgcgttcct cgagcacgtt ggcccaggcg tcgtgatccc
     3181 actcgacgcg cacgcggacg gcggcggcct cggggccggc ttcgaggtgg taggtgtcgc
     3241 tcgcggtgcg gtggaccgcg cgcacaccag ccgcgggcgt atcgaccgcg agcgtgtgag
     3301 cctcgcggac cagcacgccg cgggggctgc gggtctcggc ggcgaggagc tgcagggcgc
     3361 gggcctcgtc ttgctggccg aggtcgtagc ttcgcgtgat ggtggcggcc tcggtgtagg
     3421 cgtcgccggt cgtctcgtcg cgcagctcgg cgaagccgcg aaattcgccg cgggcggggt
     3481 cgtaccagcc atcgcgtagg cggcggcgca gcgtgctcga ccagccggcg ccgtcgtcct
     3541 cacgggtctc ggtgagcacg ggcatcgcct ccggcggcgc tgtcgtccac ggcgtacccg
     3601 cggccgcgtc ctccgcggcg agctgcgcgg ccgagcggta ggtgtgctcg cgcgagtagc
     3661 cgaggccatt ttcgaagcgt gagagcaggc cggggcgctc ggagaacacg gaccacgcgc
     3721 gccagggctg cgaaccgtcc gtgtcggcgc gcaggatatc cagcgtgccg ctgccgtcga
     3781 tgtccgtgac gaggacaacc tcgtcggcct cgagcgcggg ccaggcgacg ccgcccgcct
     3841 cggcgaagct gccgtccagt tcattgatcc acaggccgag ctggacctgg ccgatgcgga
     3901 gcagatcggc cgcaccgtcg ccgttgatgt ccgcgatctc ccagcgatcg gtctgcatca
     3961 tcggcgggac accgaccatg tcggccggat cctcgaacaa cccgaggccc gcacctgccg
     4021 cgacgaggat gcggttgtct tcagtgatca catggaccag atcggggatc cggtcgccgt
     4081 tcatgtcggc gagctggaca cctggctcac ccaaacgcat cccggcggga ggtggcggga
     4141 cgacctccgc gggttcatac ccggcggtgt cgcgcaggcg cagccagcac cagccgtccg
     4201 cgtcgtcatg gcgcagcacg tccacgcggc cgtccaggtt catgtcggcg agcgcgacgc
     4261 ggggatcggt cagatcgaag ctcagatcga gcgtgatcgg cgaagcggcg ccgaagggcg
     4321 tcgcgccacc gccgacgaag ctccacagct cgccgggggt cggctgcgcg agcaggtcct
     4381 gcacgccgtc gccggtgaga tcggcgaagc gggtcgtcgg tgtgagcgac acggcgggcg
     4441 acggaaggtc ggtccaggtc gcggccagcc gggtctcgcc gatgttcctc cggtagcgcc
     4501 acgcgccggg ctcgccgtcg aggagatcgg ggagcgcgtc gccgtccaca tcgacccacg
     4561 cgcggccgtc cgcggtgggg tcgagcgcgg gagcgccggt cacgtcgcgc gccgcgggca
     4621 tcacggcctc gccggt
//
LOCUS       DASWNJ010001122         2355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_690887, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001122 DASWNJ010000000
VERSION     DASWNJ010001122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690887"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VGB85_18910"
     CDS             complement(<1..312)
                     /locus_tag="VGB85_18910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136167.1"
                     /translation="MPARSSARISARLGAAGSGGCSTRPADRDEKEQRQAARIRSHIG
                     WSFRAVYWNREPRAAQDGFLFAAVFFAVFFFAAVFFAAVFFAAGFFFAALFAGFFAAL
                     FA"
     gene            311..514
                     /locus_tag="VGB85_18915"
     CDS             311..514
                     /locus_tag="VGB85_18915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136168.1"
                     /translation="MTRDEFYTVLDEIVVERGLRMSLPTNDDRLVALPVAEGGELLCF
                     MPTGVLNYVEYKGGIFLEKTDGE"
     gene            complement(536..2119)
                     /locus_tag="VGB85_18920"
     CDS             complement(536..2119)
                     /locus_tag="VGB85_18920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136169.1"
                     /translation="MRPYHASLAVLLATAACGDSGSTTASASDSASGTGDTGAAASTG
                     AATGTGTDTPTTGDAGSQSASDSVGTTSTGTTGATEASGSGDSGSTTASTGDLTSADT
                     TGSLSASDTGTTQDDSGTGSSTGELCVDECAEGQSQCDASGEQQCVVNDLGCTAWSDP
                     VPCDPGEVCVGDSCGLGCADECDPGSKQCVDNGVSSCVDDPMTTCTVWSDPVACAPGK
                     VCEAGTCVTPPALCLEDCVFQKQQVNASVDFYSVWGSSAKAVWTVGASGTALYYNGVT
                     WKAVDSGVGTRLECVHGSAANDVYAITGDAQVIRWDGTQWLPHIDLNPTWGESACLSV
                     IGTKDLLALVYDSNGSKVHLYRIKDGVKTLLDTATPQDVYTPDGSKIASMSLRAFSAT
                     AALATADRAYRWTSPDLVNMAAPNPSYGLWALAPDFAYAAASHSGIGHRWDGQSWKIV
                     NPGLNGYLHMFTGTAKDRIFGVGETKVGTIAAIVSFDGIGWSPAVTPADAKSLYAGWS
                     APTGEVFAVGKAGTILIGK"
     gene            complement(2151..>2355)
                     /locus_tag="VGB85_18925"
     CDS             complement(2151..>2355)
                     /locus_tag="VGB85_18925"
                     /inference="COORDINATES: protein motif:HMM:NF024639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEY0136170.1"
                     /translation="AALGLPASECVMIGDTRYDLEAARRAEIPAIAFLCGGMPEDTLG
                     DSLAIYDGPADLLEHLDASPLMA"
BASE COUNT          356 a          781 c          844 g          374 t
ORIGIN      
        1 cgcgaagagt gcggcgaaga agcccgcgaa gagtgcggcg aagaagaagc ccgccgcgaa
       61 gaagaccgcc gcgaagaaga ccgccgcgaa gaagaagacc gcgaaaaaga ccgccgcgaa
      121 gaggaagccg tcctgagcag cgcgcggttc tctattccaa tatactgccc tgaaggacca
      181 accaatgtgg gatcgaatcc gtgctgcctg gcgttgctcc ttctcgtcgc ggtcggcagg
      241 gcgcgtcgag cagcctcccg atccggcggc gcccagcctc gccgagatgc gcgcgctgct
      301 gcgggcgggc atgacgcgcg acgagttcta tactgtgctc gacgagatcg tcgtggagcg
      361 ggggctccgg atgtcgctgc cgaccaacga cgatcggctc gtcgcgctgc cggtggcgga
      421 gggcggcgag ctgctgtgct tcatgcccac cggcgtgctc aactacgtcg aatacaaggg
      481 cggcatcttc ctcgagaaga cggacggcga gtgagacaca gcccgccccc gatatctact
      541 tgccgatgag gatggtgccg gccttgccga ccgcgaacac ctcgccggtc ggggcggacc
      601 agccggcgta cagggacttg gcgtcggccg gcgtgaccgc gggcgaccag ccgatgccgt
      661 cgaagctgac gatcgccgcg atcgtaccga ccttggtctc gccgaccccg aagatgcgat
      721 ccttggcggt cccggtgaac atatgaagat atccgttgag cccggggttg acgatcttcc
      781 agctctggcc gtcccagcgg tggccgatgc cgctgtggct ggctgccgcg taggcgaagt
      841 cgggggcgag ggcccataac ccataagagg ggttcggggc ggccatgttg acgaggtcgg
      901 ggctggtcca gcggtaggcg cggtcggcgg tggcgagcgc ggcggtggcc gagaatgcgc
      961 gcagcgacat gctggcgatc ttgctgccgt cgggcgtata tacgtcctgt ggcgtcgccg
     1021 tatcgagcag cgtcttgacg ccgtccttga tgcgataaag gtgcaccttg ctgccgttcg
     1081 agtcatagac gagcgccagc aggtccttgg tgccgatcac ggacagacac gcggactcgc
     1141 cccaggtcgg gttgaggtcg atgtgaggca gccactgcgt gccgtcccag cggatgacct
     1201 gggcgtcgcc ggtgatcgcg tagacgtcgt tggcggcgct gccatgcaca cattcgaggc
     1261 gcgtgccgac gccgctgtcg acggccttcc aggtgacacc attataataa agcgccgtgc
     1321 cgctggcgcc gaccgtccac accgccttgg cgctgctgcc ccacacgctg taaaaatcga
     1381 cgctcgcgtt gacctgctgc ttctgaaaca cgcaatcttc caggcacagc gcgggcgggg
     1441 tgacgcaggt gccggcctcg cagaccttgc cgggagcgca cgcgaccggg tcgctccaca
     1501 ccgtgcaggt cgtcatggga tcgtcgacgc agctcgacac gccgttgtcg acgcattgct
     1561 tgctgccggg atcgcactcg tcggcgcagc ccaggccgca ggagtcgccg acgcacacct
     1621 cgcccgggtc acacggcacc gggtcgctcc acgcggtgca gccgaggtcg ttcaccacgc
     1681 actgctgctc gccgctggcg tcgcactgcg actggccctc ggcgcactcg tcgacacaga
     1741 gctcgccggt gctgctcccg gtgccgctgt cgtcctgcgt ggtcccggtg tcgctggcgc
     1801 tcagcgagcc ggtggtatcg gcgctcgtga ggtcgccggt gctcgcggtc gtcgagccgc
     1861 tatcgccgga accactggcc tcggtggccc cggtcgtgcc ggtgctcgtg gtcccgacgc
     1921 tgtccgacgc gctctggctg ccggcgtcgc cggtggtcgg cgtgtccgtg ccggtgcccg
     1981 tcgcggcgcc cgtgctcgcc gcggcgccgg tgtctccggt gcccgaggcg ctgtcgctcg
     2041 cggacgcggt ggtcgagccg gagtcgccgc aggcggcggt ggcgagcagg acggcgagcg
     2101 atgcatgata agggcgcatc ctggcggtat cacccgcgca gggtccgcgg tcaagccatg
     2161 agcggcgagg cgtcgaggtg ctcgagcagg tcggccgggc cgtcgtagat cgccagcgaa
     2221 tcgcccaggg tgtcctccgg catgccgccg cacaggaacg cgatcgcagg aatttccgcg
     2281 cggcgggccg cctcgaggtc gtagcgggtg tcgccgatca tcacgcactc gctggccggc
     2341 agcccgagcg cggcg
//
LOCUS       DASWNJ010001123         2000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_690968, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001123 DASWNJ010000000
VERSION     DASWNJ010001123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690968"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..859)
                     /locus_tag="VGB85_18930"
     CDS             complement(125..859)
                     /locus_tag="VGB85_18930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136171.1"
                     /translation="MLSAAFGCTIDLGGGTSTSTTDGPTTEPDTTADTTTGTPTTGAP
                     TTGVTATDTGTTDTGSDPAWCNGFDPKAAGLTVHNNDDVPLMNGSDLAAECGGQGIIM
                     IPIFPHFGGFVPAGDAVTFDVVLDVDGFNIGPGDHFFAATGLSHQVDCGDEDTYGYYF
                     GSYAFIPMFPPDGVPDINLIDGKAGHLSLTLHTPDGDVPFAADVVMKAQIDACGYGGY
                     ETSDYDDTDSDSTSDSTSGSTSGSTG"
     gene            complement(971..>2000)
                     /locus_tag="VGB85_18935"
     CDS             complement(971..>2000)
                     /locus_tag="VGB85_18935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136172.1"
                     /translation="HDSGDDTGTATTTLAPTTSSTSTGSTTEHTPTGEPDTGTTTAWE
                     LGLEIGSDRGALLSVWGPAPDEVYAVGGQQASPTESSGILYKFDGATWSEQSLPPGTP
                     MLHWIHGVDGDLWTVGRDGTTMRREGDEWVVHPTGVDTILWGVWGPRSDLLWTVGGDG
                     VDDDPVMLRWDGVAWASVPLPATDKSGGLFKIWGTAADDVTAVGDRGLALHFDGDAWT
                     AQPTDDLADLISVWGRGPNEHLAVGGRANGRLARWDGAAWSGVTLPVPGLSGVWMDPE
                     GAATVVGMQGTIGEVAPGGLDIVAQASPTYLLLHAVFGFADGPRFAVGGNLAGVPPYV
                     GVIVQRGP"
BASE COUNT          280 a          652 c          751 g          317 t
ORIGIN      
        1 gtgaccggtg cggtgatcgt gtcgccggtg ctcgccacgt cggtgtcgct ggtgttcgtg
       61 ccaccgccgc aggcggcggc gagcgtgagg gcgaggggga ggtggcgcat ggcggtatcc
      121 taactcaacc cgtgctgccc gaggtgctgc ccgaggtgct gtccgaggtg ctgtccgagt
      181 cggtgtcgtc gtagtcggag gtctcgtagc cgccgtagcc gcacgcgtcg atctgcgcct
      241 tcatgacgac atcggccgcg aagggcacgt cgccgtccgg ggtgtgcagg gtcagcgaga
      301 ggtgcccggc cttaccgtcg atcaggttga tgtcggggac gccgtcgggc gggaacatcg
      361 ggatgaatgc atacgacccg aagtagtacc cgtaggtatc ctcgtccccg caatccacct
      421 ggtgcgagag gccggtcgcc gcgaagaagt ggtcgcccgg gccgatatta aaaccgtcga
      481 cgtcgagcac gacatcgaag gtgacggcgt cgccagcggg cacgaagccg ccgaagtggg
      541 ggaaaatcgg gatcatgatg atgccctgac caccacactc ggccgcgaga tccgagccgt
      601 tcatcagcgg cacgtcgtcg ttgttgtgca ccgtcaggcc ggccgccttc gggtcgaagc
      661 cgttgcacca cgcggggtcg ctgccggtgt cggtggtgcc cgtgtcggtc gccgtgacgc
      721 cggtggtcgg cgcgccggtg gtcggtgtgc cggtggtggt gtccgcggtg gtgtccggct
      781 ccgtggtcgg gccgtcggtc gtgctcgtgc tggtgccgcc gccgaggtcg atggtgcagc
      841 cgaaggcggc gctgagcatg agggtcgctg aggtcggtcg aatgttcata agccagtggc
      901 gcgcgtcgat gcggcgcagt gacagtgtag cgaaggccga gggtcgatag agcgcgacgc
      961 gaccgcgcga tcagggcccg cgttgcacga tcacgccgac gtacgggggg acacccgcga
     1021 ggttgccacc gaccgcgaag cgcgggccgt ccgcaaatcc gaacacggcg tgcagcaaca
     1081 ggtaggtcgg gctggcctgc gcgacgatgt cgaggccgcc gggcgcgacc tcgccgatcg
     1141 tgccctgcat gccgacgact gtcgcggcgc cttccgggtc catccacacg ccgctgaggc
     1201 ccggcaccgg caaggtgacg ccgctccatg ccgcgccgtc ccagcgcgcg agtcgaccgt
     1261 tggcgcggcc gccgacggcg aggtgctcgt tcggcccgcg accccacacg ctgatcaggt
     1321 cggcgaggtc gtcggtcggc tgggcggtcc acgcgtcacc gtcgaagtgc agcgcgaggc
     1381 cgcgatcacc gacggcggtg acatcgtcgg cggcggtgcc ccaaattttg aataagccgc
     1441 cgctcttgtc ggtcgcgggc aaggggacgg aggcccacgc gacgccgtcc cagcgcagca
     1501 tcaccgggtc gtcgtcgacg ccgtcaccac cgacggtcca caggaggtcg ctgcgtgggc
     1561 cccacacgcc ccacaggatc gtgtcgacgc cggtcgggtg cacgacccat tcgtcgccct
     1621 cgcgccgcat ggtggtgcca tcacgaccga cggtccacag gtcgccgtcg acgccgtgga
     1681 tccagtgcaa catcggcgtg ccgggcggga gcgattgctc gctccacgtc gcgccgtcga
     1741 acttatataa aatgccgctc gactcggtcg gagaggcttg ctggccgccg accgcgtaca
     1801 cctcgtccgg cgccggaccc cacacgctga gcagggcccc gcggtcgctg ccgatctcga
     1861 ggccgagctc ccaggcggtc gtggtgccgg tgtcgggctc gccagtcggg gtgtgctcgg
     1921 tggtcgagcc ggtggaggtc gagctcgtgg tcggggcgag cgtggtggtc gcggtcccgg
     1981 tgtcgtcgcc ggagtcgtgg
//
LOCUS       DASWNJ010001124         1709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_20948, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001124 DASWNJ010000000
VERSION     DASWNJ010001124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20948"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="VGB85_18940"
     CDS             <1..921
                     /locus_tag="VGB85_18940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HEY0136173.1"
                     /translation="LVWRPAAPLKPGAAHQVTGGFDNPDSIEDYCADDLLALDFEFTA
                     DMGPSQPLPTPEVSAVESVETTDIESLTTLVCCDGAFPEEGQFDCGYDGDYVFWNDGA
                     CAPTASQGALSVQLEAPLAVAPSTAALVVTSVRVDGEVVRTGLAPTATYSLYSPFCTE
                     VEQRNLATGETVTSAKQCHGDAVVAQLGPQPLDPALVLAKECTGPAYVCEVTEDAYPQ
                     WDPDKCTPYEPAQETETPTTSDSDSPTTTASESDTSSSSSSSSSDTAGETPDDNRGCA
                     CTTTPADPTPLLGLLGLGLLRRRRPARKDR"
     gene            1019..1354
                     /locus_tag="VGB85_18945"
     CDS             1019..1354
                     /locus_tag="VGB85_18945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136174.1"
                     /translation="MFDEKTASPEEIAARLHELDQEVHQLITAKLGLIDELIPAKSEQ
                     ELLALSRRAEALATATARLFSEQEHLMELLMALSGDDAPPQDGPDLAVGESPNPREQP
                     AGFDPDEVD"
BASE COUNT          302 a          633 c          541 g          233 t
ORIGIN      
        1 ctggtgtggc ggcccgccgc gccgctcaag ccgggcgcgg cccaccaggt caccggcggc
       61 ttcgacaacc ccgacagcat cgaggactac tgcgccgacg acctgctcgc gctcgacttc
      121 gagttcaccg ccgacatggg cccgtcgcag ccgctgccca cgcccgaggt ctcggccgtc
      181 gagtccgtcg agaccaccga catcgagtcc ctcacgaccc tggtctgctg cgacggcgcg
      241 ttccccgagg agggccagtt cgactgcggc tacgacggcg actacgtgtt ctggaacgac
      301 ggcgcctgcg cgccgacggc cagccagggc gcgctctccg tgcagctcga ggcgcccctc
      361 gcggtcgccc cctcgaccgc cgcgctcgtc gtcaccagcg tgcgcgtcga cggcgaggtc
      421 gtgcgcacgg gactcgcgcc aacggccacg tatagcctgt acagcccctt ttgcaccgag
      481 gtcgagcagc gcaacctcgc gaccggcgag accgtgacct ccgccaagca gtgccacggc
      541 gacgccgtcg tcgcccagct cggcccacaa cccctcgacc cggccctcgt gctcgccaag
      601 gagtgcactg gcccggccta cgtctgcgag gtcaccgagg acgcctaccc gcagtgggac
      661 cccgacaagt gcaccccgta cgagcccgcg caggagaccg agaccccgac gacctccgac
      721 agcgacagcc cgacgaccac cgccagcgag tccgacacca gcagcagctc cagcagcagc
      781 agctccgaca ccgctggcga gaccccggac gacaaccgcg gctgcgcctg caccacgacc
      841 cccgccgacc cgaccccgct gctcggcctg ctcgggctcg gtcttctgcg ccgccgccga
      901 cctgctcgaa aggacaggta gcttcgaggg ctgagcgtat tcgtggagaa ccgtggcctc
      961 accagtccgc gacccgaggg accccgatga gcgcgggacc agggccggag tgagcatcat
     1021 gttcgacgag aagacagcca gtcccgaaga gattgcagca cgcctccatg aattggacca
     1081 ggaggtccat cagctcatca cggcgaagtt ggggctgatc gatgagctca tccccgcgaa
     1141 gtcagagcaa gaattgctcg cgctgagccg ccgcgcggag gcgttggcga ccgcaacggc
     1201 gcggctcttc tcggagcagg agcacttgat ggaattgctc atggcattgt ccggagacga
     1261 cgcacctcct caggacggcc ctgacctcgc tgttggcgag tcgccgaacc cgcgagagca
     1321 gcccgccggg ttcgatcccg acgaagtgga ctaactacgg tccagtcgcg cactgccggt
     1381 gatcgccttg tgactcgaag cgcggggagc cctccgcggg ggcagcggag tggcggtgag
     1441 gcgaagctcc tggagggccg aatctgagga gaacaagagc accgcggggc gtttaaaaaa
     1501 cgcggcgcgg acttccgcaa cacacgagcg tcttcgccct tgaccgcgcg gatctgctgt
     1561 ggcacctaca ctgaacacct gaccatgccg acgtcaaacg ccgattccag cagtgaagcc
     1621 ctgtccccgg cgatccgcga gctgctctcc atcttcgagg gcgagctctc cggggtctcc
     1681 tttccgggcg tcgaccgcaa gaccctgac
//
LOCUS       DASWNJ010001125         2741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_479292, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001125 DASWNJ010000000
VERSION     DASWNJ010001125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479292"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VGB85_18950"
     CDS             complement(<1..303)
                     /locus_tag="VGB85_18950"
                     /inference="COORDINATES: protein motif:HMM:NF024646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136175.1"
                     /translation="MTDGVGIVPSPDSQDMSSGTGDSLRAEAEAAYARALGHLRSVQQ
                     PKGALAGEVVWNPMLVCQYVVLCQILGVPIAEDRKRRIRRSLELQVRPDGGWGMHPD"
     gene            complement(300..1055)
                     /locus_tag="VGB85_18955"
     CDS             complement(300..1055)
                     /locus_tag="VGB85_18955"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0136176.1"
                     /translation="MQRASPPLATRRRIASRRATSSRAAALVIAALTLGLGASACRKG
                     ELVANMDYDDPKQAIAEMDDAKRDPWVMPDKVVRALPIASKDSIIADIGAGSGYFSRR
                     LAREVPGGTVYAVDVDDEFRGFIEQNRESWGTPNIEPHLAFYDDPALPEHGVDLVFVS
                     NTYPYLRSRVSYFRKVAGALKKGGHLVVINFKPDAQAPDNTAPAPQFRTSRETVISEL
                     AQAGFTLTREETFLPYQYFLIFQIGAAKAAATP"
     gene            1184..1987
                     /locus_tag="VGB85_18960"
     CDS             1184..1987
                     /locus_tag="VGB85_18960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136177.1"
                     /translation="MSRAERHALGRRLVAEGSVLWSALPVEWPTEGAKGSVADPPELP
                     GLAWRIWASLSSLLRRPRQSFAYVHEPVAHGAVLRLLHGVRLPLWLVLLVALGLRAWL
                     SPNGATALAPIHEVLDPRLCEVLSLWLLLMVPIGVPLLYFALGMATHVALTLTGGAPR
                     SIAASMRAAGTALAPGLLGISILDLFVHLGMMSGLAYLIALAVVGLSYFHQLGQALTG
                     THGISPIRGYVVAVVPLAIVLAVQLGRALLVLPDFPGWEPSPTHPYFIP"
     gene            complement(2057..>2741)
                     /locus_tag="VGB85_18965"
     CDS             complement(2057..>2741)
                     /locus_tag="VGB85_18965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136178.1"
                     /translation="LAVALAAGGCDNKAEPAPAKTAEVKAEAKPEATNPEATTPEVAK
                     PADDRVLADSGLGVGGKFKPFDIVNCESGDQYCQVCKFGGSPKIMAVGTIDDPAFKTD
                     LQNLDALVQKYGEDKIKAFAVVTDIKDGKSTTPLADREALIAKVKALKTELGLKLPLT
                     VPAAEDGAPNKAFDDYYNVTKSRTLMFADGRNSVKYSAVAPSDLSELNKAIESVVGAA
                     GAPAPTPAG"
BASE COUNT          331 a          951 c         1001 g          458 t
ORIGIN      
        1 atcggggtgc atgccccagc cgccgtccgg gcgcacctgc agctcgaggc tgcggcggat
       61 ccggcgcttg cggtcctccg cgatcggcac gccgaggatc tggcatagca ccacgtactg
      121 gcacaccagc atcgggttcc acaccacctc gcccgcgagc gcgcccttgg gctgctgcac
      181 cgagcgcagg tgcccgagcg cccgcgcgta cgcggcctcg gcctcggcgc gcagggagtc
      241 gccggtcccc gaggacatgt cctgcgagtc aggcgacgga acgatcccga cgccgtcggt
      301 catggcgtcg cggccgcctt cgccgcgccg atctggaaga tgagaaagta ctggtagggc
      361 aagaaggtct cctcgcgcgt cagcgtgaac ccggcctgcg ccagctcgct gatcaccgtc
      421 tcgcgtgagg tgcgaaactg cggcgccggg gcggtgttgt ccggcgcctg cgcgtccggc
      481 ttgaagttga tgacgaccag gtggccgccc ttcttgagcg ccccggcgac cttgcgaaag
      541 taactgacgc ggctgcgcag gtacgggtag gtgttcgaca cgaacacgag gtcgacgccg
      601 tgctcgggca gcgccgggtc gtcgtagaag gcgaggtgcg gctcgatgtt cggcgtgccc
      661 cacgactcgc ggttttgctc gataaagccg cggaactcgt cgtcgacgtc gaccgcgtac
      721 acggtgccgc cggggacctc gcgggcgagg cggcgcgaga agtagcccga gccggcgccg
      781 atgtcggcga tgatcgagtc cttgctggcg atcgggaggg cccgcacgac cttgtcgggc
      841 atcacccagg ggtcgcgctt ggcgtcgtcc atctcggcga tcgcctgctt gggatcgtca
      901 taatccatgt tggcgaccag ctcgcccttg cggcaggcgc tggcgccgag gccgagggtc
      961 agggcggcga tcaccagggc cgcggcacgg gaggaggtcg cgcgacgcga agcgatccgg
     1021 cggcgtgtgg cgaggggagg ggaggcacgc tgcatgcgcc gagggtagtc ccgccgccgg
     1081 gcgctgtcga ccgtgggggc atgcaggggc tggcagactc gggtagactc ccgcggcctt
     1141 ggcggacccc acagcagcgc aggaccctcc acgccccacc gccatgtcca gggccgagcg
     1201 ccacgccctc gggcgccgcc tggtcgccga gggctcggtc ctgtggagcg ccttgcccgt
     1261 cgagtggccg accgagggcg ccaaggggtc ggtcgccgac ccgcccgagc tcccgggcct
     1321 cgcctggcgg atctgggcca gcctgtcgtc gctgctgcgg cgcccgcggc agagcttcgc
     1381 gtatgtgcac gagccggtgg cccacggcgc cgtgctgcgc ctgctgcacg gcgtgcgcct
     1441 gccgctgtgg ctggtgttgc tggtcgcgct cggcctgcgc gcctggctgt cgccgaacgg
     1501 cgccaccgcg ctcgccccga tccacgaggt cctcgacccg cggctgtgcg aggtgctctc
     1561 gctgtggctg ctgctgatgg tcccgatcgg cgtgccgctg ctgtacttcg cgctcggcat
     1621 ggcgacccac gtcgcgctga ccctgaccgg cggcgccccg cggtcgatcg ccgcgagcat
     1681 gcgcgcggcc gggaccgccc tggcccccgg cctgctcggg atctcgatcc tcgacctgtt
     1741 cgtgcacctg ggcatgatgt cgggcctcgc ctatctgatc gccctcgccg tcgtcggcct
     1801 gtcctacttc caccagctcg gccaggcgct gaccggcacc cacggcatct cgccgatccg
     1861 cggctacgtg gtcgccgtcg tgccgctggc gatcgtcctc gcggtccagc tcggccgcgc
     1921 cctgctggtg ctcccggact tccccggctg ggaaccatcg ccgacgcacc cgtacttcat
     1981 cccatgatcg ggtgatcgga tgacgcacga acgccgcgcg aacgaggttc gcgcggcgtt
     2041 tcattgcgcg gggatctcag cccgcgggcg tcggggcggg cgcaccggcg gcgccgacca
     2101 ccgactcgat cgccttgttg agctccgaga ggtcgctcgg ggcgacggcg gagtatttca
     2161 cggagttgcg gccgtcggcg aacatcaggg tgcggctctt ggtgacgttg taataatcgt
     2221 cgaaggcctt gttgggggcg ccgtcctcgg ccgcggggac cgtgagcggc agcttgaggc
     2281 cgagctcggt cttgagggcc ttgacctttg cgatcagcgc ctcgcggtcg gcgagcggtg
     2341 tggtcgactt gccgtccttg atgtcggtga cgacggcgaa cgccttgatc ttgtcctcgc
     2401 cgtacttctg gaccagcgcg tcgaggttct gcaggtcggt cttgaaggcc gggtcgtcga
     2461 tcgtgccgac ggccatgatc ttcgggctgc cgccgaactt gcacacctgg cagtactggt
     2521 cgccgctctc gcagttgacg atgtcgaagg gcttgaactt gccgccgacg ccgaggccgc
     2581 tgtcggcgag cacgcggtcg tccgcgggct tggcgacctc gggggtcgtc gcttcggggt
     2641 tagtcgcctc gggcttggcc tcggccttga cctcggcggt cttcgccggg gccggctcgg
     2701 ccttgttgtc gcagccgccg gccgcgaggg cgacggcgag g
//
LOCUS       DASWNJ010001126         8032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_159914, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001126 DASWNJ010000000
VERSION     DASWNJ010001126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 8032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8032
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159914"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VGB85_18970"
     CDS             complement(<1..872)
                     /locus_tag="VGB85_18970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0136179.1"
                     /translation="MSLLLACGRGELAVTDGDSSGDSSGVATTTTSPTDPTTPTGDPT
                     TDAPTTGNGDATIEPTTAVMTEPTTVDPTTGEPTGTSTGEDTSSTSDTSTTSGDSTST
                     GDTDVSSSGDELVCGDGIVGPGEACDDANLDDTDECLGNCQAATCGDGALQAGVETCD
                     DGNVDDTDACTSACVPAICGDGLVQAGVEGCDDANQVDDDACSNACVPADCGDGILQG
                     GIEECDDGNDLDTDTCTNMCKLAVCGDTVVQEGVELCDEGGETLACNADCTPASCGDL
                     IVNMSAGEACDAGA"
     gene            complement(1006..1251)
                     /locus_tag="VGB85_18975"
     CDS             complement(1006..1251)
                     /locus_tag="VGB85_18975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136180.1"
                     /translation="MITLYNKDSGAVIGPLSDKQLADLIDLLVEESADDQDYYIDSTT
                     LDYLAEQGADPALLEMLRPHVPEGEEGIEIEWRSDAT"
     gene            complement(1304..2701)
                     /locus_tag="VGB85_18980"
     CDS             complement(1304..2701)
                     /locus_tag="VGB85_18980"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEY0136181.1"
                     /translation="MAIRIIPGAVGRTSVRALVLTDQTTPPALTLVNVRGGPVPALTI
                     KQHGSGDERFDQHVIGAGVRMYLATAEGLRPGADYQIDRTHFRTLPAKLPTDGFRLAL
                     ASCYSDDFKRADDYLKVLQGSTTFGQLQAKLLVGDNVYVDVGSIGAQARSGFEETAER
                     YLRHFWRSDYADVLSYLPTFTMWDDHEFWNNFPESQFWLPRSMHSRHDEFRDAALAGI
                     KAFQAVLNPPPVARHGLSYRFEIPPLSVFVLDLRAGRSRHEVADPIMCSEAELAAFER
                     WAAKLEGPGALVVGQPLWQGAGDWQDWNPPAFPAQYARIWKALTDAPFDIAVLSGDVH
                     HSRLVEIDVGGDRRVWELISSPACHIPTVESIAARAFDVQDRGRVGFPAEIPGAGGAR
                     LAAYHMGTGIPNTIALLKLTPVEDDGVAFSGSFVDLVRKTVAPWDAPPPGIPIAAGDH
                     AWCKREPLFVLRRRS"
     gene            2756..3556
                     /locus_tag="VGB85_18985"
     CDS             2756..3556
                     /locus_tag="VGB85_18985"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0136182.1"
                     /translation="MPTLIRDGQRVVYSALGSGPNTVLLVHNLLSQRGSFAEVASHLA
                     HRCRAVAVDLRGHGESGGAPRAFSVHDLAEDLVAVMDALAVERAVLVGTSLGATVSAL
                     VAVEQPSRVSGLVLMSATPHAASARDRLRFASLATVLRTLGPGPVMPAILGQLFGASY
                     RRRAAEEVAATAAQIRATARRDLAWSIRAWTGRPELAGRLRGIEAPTRVVIGAEDTAC
                     PRRFGEAIAAEVPGATLHVVAGSGHSLQLEQATAVAAVIDGLLGQLPP"
     gene            complement(3581..4267)
                     /locus_tag="VGB85_18990"
     CDS             complement(3581..4267)
                     /locus_tag="VGB85_18990"
                     /inference="COORDINATES: protein motif:HMM:NF020135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0136183.1"
                     /translation="MTRDHGWEALLGPAGDPLLVCVRNDDLAAVVERVPVMRRSDLVF
                     VQNGMIDGWLASHGLANSTRGVLFFAVAKRGGPIEPGGVSPFTGPHAPALADWLLSLG
                     VPAEAVDRGQFAAVMLEKLIWNSAFGLLCEYYGVTVGEVLERHAEELTRVTAELNAVG
                     RAALGVTLEDPPLLARLRNFSARIPDNRAGVKEWSWRGGWFVTAAREHGLSMSTHDRL
                     LAGLPRPPTG"
     gene            complement(4553..7513)
                     /gene="uvrA"
                     /locus_tag="VGB85_18995"
     CDS             complement(4553..7513)
                     /gene="uvrA"
                     /locus_tag="VGB85_18995"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY0136184.1"
                     /translation="MDAVTRPDLPAPSVAPATIAAPPASAPSSPPANKEPVREPDFIE
                     IRGAHEHNLHIDELKLPKRKLVVFTGVSGSGKSSLAFDTLYAEGQRRYIESLSAYARQ
                     FLGQLERPKVESLRGLSPTIAIEQKSASNNPRSTVGTITEIYDYLRVLYARVGVQHCT
                     ECGKPVRSSSPEQIVEELLHEAKDARVVLLAPVVTHRKGEYKDLFEELRGRGFARVEV
                     DGEIHPLDTPPKLDKKLKHNIAVVVDRVIARASDRRRITESVELALREGHGEVRAESA
                     DTTGPAAGFKRLFSEARSCCGKSFPELNPQSFSFNSPLGMCPTCNGLGTRMEAAEDLV
                     VPDTSLSIRDGAIAPWASAMDRGEGWTFRIVEALAKSCQVDLDTPWNKLLKKKRTLVL
                     YGAEAGAKRIAVEFGSEGGNSHGTFGMRYEGVLPNLMRRFRETDSDAAREFLRKFMRE
                     ANCDSCGGRRLRNESLFVRVAKTNLAESTAMTVRQAADHFAGLGLTGAQQTIGEGALR
                     EIGSRLSFLLNVGLDYLTLDRSGPTLSGGEAQRIRLASQLGSELSGVMYVLDEPSIGL
                     HQRDNQRLIRTLQHLRDLGNSVVVVEHDEDTIRAADHLVDFGPGAGHLGGEVVFSGPP
                     DEVEACERSLTGAYLSGRKKIEVPATRRKPRAAITVRGASEHNLQNVDVAFPLGVLVA
                     VTGVSGAGKSSLVNGILLPSLSQKLHGSLERVGKHKSIDGLGALDKVIAIDQRPIGRT
                     PRSNPGTYTKSFDQIRELFAQLPDSRARGWKAGRFSFNVKGGRCEACQGDGVIKVEMH
                     FLSDVYVPCEVCNGKRYNAQTLGVTYKGRNIAEVLDLSVDDCLEVFANVPPLKRILQT
                     LVDVGLGYMKIGQTAPTMSGGEAQRVKLSRELCKVQTGNTLYVLDEPTTGLHFEDIHR
                     LLLVLQRLVDAGNTVLVIEHNLDVVKCADWIIDLGPEGGSNGGRVIAEGTPEQVAKIK
                     GSFTGEFLRPLLGR"
     gene            7697..7775
                     /locus_tag="VGB85_19000"
     tRNA            7697..7775
                     /locus_tag="VGB85_19000"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7730..7732,aa:Leu,seq:taa)
BASE COUNT         1111 a         2656 c         3020 g         1245 t
ORIGIN      
        1 tccgcgcccg cgtcgcaggc ctcgccggcg gacatgttga cgatcaggtc gccgcagctc
       61 gcgggggtgc agtcggcgtt gcacgcgagg gtctcgccgc cctcgtcgca cagctcgacg
      121 ccctcctgca cgacggtgtc gccgcagacg gcgagcttgc acatgttggt gcaggtgtcg
      181 gtgtcgaggt cgttgccgtc gtcgcactcc tcgatgccgc cctgaaggat gccgtcgccg
      241 cagtcggccg ggacgcaggc gttcgagcag gcgtcgtcgt cgacctggtt ggcgtcgtcg
      301 cagccctcga cgccggcctg gaccagcccg tcgccgcaga tcgcaggcac gcaggcgctg
      361 gtgcacgcgt cggtgtcgtc gacgttgccg tcgtcgcagg tctcgacgcc ggcctgcagc
      421 gcgccgtcgc cacaggtggc cgcctgacag ttgccgaggc actcgtcggt gtcgtcgagg
      481 ttggcgtcgt cgcaggcctc gcccggaccg acgatgccgt cgccgcagac cagctcgtca
      541 ccgctgctgc tgacatcggt gtcgccggtg ctcgtgctgt cgccgctcgt ggtcgacgtg
      601 tcgctcgtcg agctggtgtc ctcgccggtg ctcgtgccgg tcggctcgcc ggtggtcgga
      661 tcgaccgtgg tcggctcggt catgaccgcc gtggtcggct cgatcgtcgc gtcaccgttg
      721 ccggtcgtcg gcgcgtcggt cgtcgggtcg ccggtcggcg tggtcgggtc ggtcgggctc
      781 gtcgtcgtgg tcgcgacgcc gctgctgtcg ccactgctgt cgccatcggt gacggcgagc
      841 tccccacgac cgcaggcgag gagcagcgac acggctgcga gggcgaagga acgacggtcg
      901 gaccgggtaa gacaagacat gcgccacagc ctaaagatgg ccgcgcggcc cttcaagcgg
      961 cgatttcagg cccgaccggc cggcgtcgtc tgccggcggg gcgcgtcagg tcgcgtcgga
     1021 gcgccactcg atctcgatgc cctcctcacc ctcggggacg tggggccgca gcatctcgag
     1081 cagcgccggg tccgcgccct gctcggcgag gtagtcgagc gtggtcgagt cgatgtagta
     1141 gtcctgatcg tcggcgctct cctcgaccag caggtcgatg aggtccgcga gctgcttgtc
     1201 gctcaggggg ccgatgacgg cgccgctgtc cttgttgtag agcgtgatca cgggccgcgt
     1261 tataccacgg gcccgcgcga ccgcgcgcac agcatccgca gcatcaactg cgccggcgca
     1321 ggacgaacag cggctcgcgc ttgcaccacg cgtgatcgcc cgcggcgatc gggatccccg
     1381 gcggcggcgc gtcccacggg gcgacggtct tgcggacgag atcgacgaag ctgccggaga
     1441 acgcgacgcc gtcgtcctcg accggggtga gcttgaggag cgcgatcgtg ttcgggatcc
     1501 cggtgcccat gtgatacgcg gcgaggcggg ccccgccggc gcccgggatc tccgcaggga
     1561 agccgacgcg gccgcggtcc tgcacgtcga aggccctcgc ggcgatcgac tcgaccgtcg
     1621 ggatgtggca cgcgggcgag ctgatcagct cccatacgcg gcggtcgccg ccgacgtcga
     1681 tctcgacgag gcggctgtgg tgcacgtcgc ccgagagcac ggcgatgtcg aagggcgcgt
     1741 cggtgagcgc cttccagatc cgggcgtact gggccgggaa ggccggcggg ttccagtcct
     1801 gccagtcgcc ggcgccctgc cacagcggct ggccgaccac cagggcgccg gggccctcga
     1861 gcttggcggc ccagcgctcg aaggcggcga gctcggcctc gctgcacatg atcgggtcgg
     1921 cgacctcgtg gcggctgcga ccggcccgca ggtcgagcac gaagaccgac agcggcggga
     1981 tctcgaagcg atacgagagg ccgtggcggg cgaccggcgg cgggttgagc accgcctgaa
     2041 acgccttgat cccggcgagc gcggcgtcgc ggaactcgtc gtgacggctg tgcatgctgc
     2101 gaggcagcca gaactgcgac tcggggaagt tgttccagaa ctcgtggtcg tcccacatcg
     2161 tgaaggtggg caggtacgag agcacgtcgg cgtagtcgct gcgccagaag tggcgcaggt
     2221 agcgttcggc ggtctcctcg aagccgctgc gggcctgggc gccgatcgat ccgacgtcga
     2281 cgtagacgtt gtcgccgacg agcagcttgg cctgcagctg gccgaaggtc gtcgagccct
     2341 ggagcacctt gaggtagtcg tcggcgcgct tgaagtcgtc ggagtagcag gacgcgagcg
     2401 cgaggcgaaa gccgtccgtg gggagtttgg caggtagggt gcgaaagtgg gtgcgatcga
     2461 tctgatagtc cgcgccgggc cgcaggccct cggcggtggc gaggtacatg cggacgccgg
     2521 cgccgatcac gtgctggtcg aagcgctcgt caccggagcc gtgctgcttg atcgtcagcg
     2581 cgggcacggg gccgccgcgg acgttgacga gggtcagcgc gggcggcgtc gtctgatcgg
     2641 tgaggacgag cgcgcgcacg ctggtgcgac cgacggctcc ggggatgatg cggatggcca
     2701 tgaaccacga tacgcccgcg acaggacggc gacaatcaca gtacgatgcg cccgcatgcc
     2761 gaccctgatc cgagacgggc agcgcgtcgt ctacagcgcg ctcggttccg gtccgaacac
     2821 cgtcctgctg gtccacaacc tgctgtcgca gcgcggcagc ttcgccgagg tcgccagtca
     2881 cctcgcgcat cgctgccggg cggtcgccgt cgacctgcgc gggcacggcg agagcggcgg
     2941 ggcgccgcgg gccttctcgg tccacgacct cgccgaggat ctcgtcgcgg tgatggacgc
     3001 gctcgcggtc gagagggcgg tgctcgtcgg gacctcgctc ggcgcgacgg tgtcggcgct
     3061 ggtcgccgtc gagcagccgt cgcgggtgtc gggtctggtg ttgatgtcgg cgacgccgca
     3121 cgcggcgagc gcccgcgacc ggctgcgctt cgcctcgctc gccacggtgt tgcgcacgct
     3181 cggccccggc ccggtgatgc ctgcgatcct cggccagctg ttcggcgcga gctaccgccg
     3241 gcgtgcggcc gaggaggtcg ccgcgaccgc ggcccagatc cgggcgacgg cgcgccgcga
     3301 cctcgcgtgg tcgatccggg cctggaccgg ccgcccggag ctcgcggggc ggctgcgtgg
     3361 gatcgaggcg ccgacgcggg tggtgatcgg cgccgaggac accgcgtgtc cgcggcgctt
     3421 cggcgaggcg atcgcggcgg aggtcccggg cgccaccctg cacgtggtcg cgggctcggg
     3481 gcacagcctg cagctcgagc aggcgaccgc ggtcgcggcc gtcatcgacg gtctgctcgg
     3541 acagctgccg ccctgagccg cctgcgaggc gacgtccgcg ctagccggtg ggaggccgcg
     3601 gcagtccggc gagcaggcgg tcgtgggtgg acatgctcag gccgtgctcg cgcgcggcgg
     3661 tgacgaacca tccgccgcgc cacgaccact ccttcacgcc ggcgcggttg tcggggatcc
     3721 gggccgagaa gttgcgcagg cgggcgagca gcggcggatc ttcgagggtc acgccgagcg
     3781 ccgcgcgtcc gaccgcgttg agttccgcgg tgacgcgtgt cagctcctcg gcgtggcgct
     3841 cgagcacctc gccgacggtg accccgtagt actcgcagag caggccgaag gcggagttcc
     3901 agatcagctt ctcgagcatg accgcggcga actgcccgcg gtcgaccgcc tccgcgggga
     3961 ccccgaggct gagcagccag tccgccagcg cgggcgcgtg cggcccggtg aacgggctga
     4021 ccccaccggg ctcgatcggc ccgccgcgct tcgccaccgc gaagaacagg accccccgcg
     4081 tgctgttcgc caggccgtgc gacgcgagcc agccgtcgat catcccgttt tgtacgaaca
     4141 cgaggtcgct gcgccgcatg accggcacac gctcgaccac cgccgcgaga tcatcgttgc
     4201 gcacgcacac cagcaagggg tcgcccgcgg gccccagcag cgcctcccag ccgtgatcgc
     4261 gggtcacgag cgtacagggc aggggcgacg ccgccgcgag ggcacccccg atccgccccg
     4321 ctccgatgat gacgacctcc ttcaaagccc caccacgata acaccccgag gcgcacgctc
     4381 gccattgccc atgtcacttg catgcccgcc gaaccccatc cacacgccct tcgccgagtt
     4441 ccggcccacc acgcagtttc tcatcggcga atctggcgca cctggctcgt acccagggac
     4501 gcagcgacga ggtctgcatg ccggtccgcc gatcctgcgg accgatatgg cctcaccgac
     4561 cgagcaaggg ccgcaagaac tcgccggtga acgacccttt gatcttcgcg acctgctcgg
     4621 gcgtgccctc cgcgatgacg cgtccgccgt tgctgccgcc ctcgggtccg aggtcgatga
     4681 tccagtcggc gcacttcacg acgtcgaggt tgtgctcgat caccagcacg gtgttgccgg
     4741 cgtcgacgag gcgctgcagc acgagcaaca gtctatggat gtcctcgaag tggaggccgg
     4801 tggtcggctc atcgaggacg tagagcgtgt tgccggtctg gaccttgcac agctcgcgcg
     4861 acagcttgac gcgctgggcc tcgccgccgg acatcgtcgg ggcggtctgg ccgatcttca
     4921 tgtagccgag gccgacgtcg acgagcgtct gcaggatccg cttgaggggg ggcacgttgg
     4981 cgaagacctc gaggcagtcg tcgacgctga ggtcgaggac ctcggcgatg ttgcggccct
     5041 tgtaggtcac gccgagggtc tgggcgttgt agcgcttgcc gttgcacacc tcgcagggga
     5101 cgtacacgtc gctgagaaag tgcatctcga ccttgatgac gccgtcgccc tggcaggcct
     5161 cgcagcggcc gcccttgacg ttgaagctga agcgtccggc cttccacccg cgggcgcgcg
     5221 agtcgggcag ctgggcgaac agctcgcgga tctggtcgaa ggactttgta taggtgccgg
     5281 ggttggaccg cggcgtgcgg ccgatcgggc gctggtcgat cgcgatcacc ttgtcgaggg
     5341 cgccgaggcc atcgatagat ttgtgcttgc cgacgcgctc gaggctgccg tggagctttt
     5401 gcgagaggct cggcagcagg atgccgttga cgagcgacga cttgccggcg ccgctgaccc
     5461 cggtgacggc gacgagcaca ccgagcggga acgcgacgtc gacgttttgc aggttgtgct
     5521 cgctggcgcc gcggacggtg atcgcggcgc gaggcttgcg gcgggtcgcc ggcacctcga
     5581 tcttcttgcg ccccgagagg tacgcgccgg tcaggctccg ctcgcaggcc tcgacctcgt
     5641 cgggcgggcc gctgaacacg acctcgccgc cgaggtgtcc ggcgccgggt ccgaagtcga
     5701 cgaggtggtc ggcggcgcgg atcgtgtcct cgtcgtgttc gacgacgacg acgctgttgc
     5761 cgaggtcgcg caggtgctgg agcgtgcgga tcagccgttg attgtcgcgc tggtgcaggc
     5821 cgatgctcgg ctcgtcgagc acgtacatca cgccgctgag ctccgagccg agctggctgg
     5881 cgaggcggat gcgctgggcc tcgccgccgg acagcgtcgg gccgctgcgg tcgagggtga
     5941 ggtagtcgag gccgacgttg agcaggaagc tcaggcggct gccgatctcg cgcagggcgc
     6001 cctcgccgat cgtctgctgg gcgccggtga ggccgaggcc ggcgaagtgg tcggcggcct
     6061 ggcgcacggt catcgcggtc gactcggcga ggttggtctt ggcgacgcgg acgaacagcg
     6121 actcgttgcg caggcgccgg ccgccgcagc tgtcgcagtt ggcctcacgc atgaacttgc
     6181 gcaggaactc gcgggccgcg tcggagtcgg tctcacggaa gcggcgcatc aggttgggca
     6241 gcacgccctc gtagcgcatg ccaaaggtgc cgtggctgtt gccgccctcg ctgccgaact
     6301 cgaccgcgat ccgcttggcg ccggcctcgg cgccgtacag caccagcgtg cgcttcttct
     6361 tgagcagctt gttccacggg gtgtcgaggt cgacctgaca ggacttggcg agcgcctcga
     6421 cgatgcggaa ggtccagccc tcgccgcggt ccatcgccga ggcccagggt gcaattgcgc
     6481 cgtcgcggat gctgaggctg gtgtcgggca cgacgaggtc ctcggcggcc tccatgcggg
     6541 tgccgaggcc gttgcaggtc gggcacatgc cgagcgggct gttgaacgag aagctctgcg
     6601 ggttgagctc ggggaagctc ttgccgcagc acgagcgggc ctcgctgaac agacgcttga
     6661 agcccgcggc ggggccagtc gtgtcggccg actcggcgcg gacctctccg tggccctcgc
     6721 gcagggcgag ctcgacggac tcggtgatcc ggcggcggtc ggaggcgcgg gcgatcacgc
     6781 ggtcgacgac gacggcgatg ttgtgcttga gcttcttgtc gagcttgggc ggggtgtcga
     6841 gcgggtggat ctcgccgtcg acctcgacgc gggcgaagcc gcggccgcgc agctcctcga
     6901 agagatcctt gtactcaccc ttgcggtggg tgacgaccgg ggcgagcagc acgacgcggg
     6961 cgtccttggc ttcatgcaac agctcctcga cgatctgctc cggcgacgag gagcggaccg
     7021 gcttgccgca ctcggtgcag tgctggacgc cgacgcgggc gtagagcacg cgcaggtagt
     7081 cgtagatctc ggtgatcgtg ccgacggtcg atcgcgggtt gttggaggcg ctcttttgct
     7141 cgatcgcgat cgtcggcgac aggccgcgca gcgactcgac cttggggcgc tcgagctggc
     7201 cgaggaactg gcgcgcgtag gccgagaggc tctcgatgta gcggcgctgg ccctcggcgt
     7261 agagcgtgtc gaacgcgagg ctcgacttgc cggacccgct gacgcccgtg aacaccacga
     7321 gcttgcgctt gggcagcttg agctcgtcga tgtggaggtt atgctcgtgg gcgccgcgga
     7381 tctcgatgaa gtccggctcc cgtacgggct ccttgtttgc cggggggctg ctcggggcgc
     7441 tcgcgggagg ggccgcgatg gtggcaggcg cgacggaggg ggcagggaga tccgggcggg
     7501 tgacggcgtc catggtcggg gtcgattcgg ggcttagcac gcgatctcgc gcaacatagg
     7561 tgtggggcgg acgcgaacga tgggccatgc ctgatagttt gcgcacgtgc tcggacgtgc
     7621 aggtgagcag agtccggtgc gacggtgttg ttgtcggggg cggcgagcct gtcctaaggt
     7681 gcagccagta aactgggccc gggtggtgga atggtagaca cgggggactt aaaatccctt
     7741 gcgcaagcgt gcgggttcga atcccgcccc gggcacccct agaaacgccg tatagactga
     7801 acaggcggtg gctttgcgcc gggtccgcaa ggcgggtgag acgacggggt cgtccggccc
     7861 cgacggcagc tcgagcggtg gtgcgatgac cacgaccacg agcccgacga gcacgacgga
     7921 tccgacgacg acggcgtgcc gacgacgacc ggggcgagcg tcgcaggcga gacggacgcg
     7981 acgacgacga tggggatcga ccggagccga tccggtgtgc ggcgactgca cg
//
LOCUS       DASWNJ010001127         7784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_617152, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001127 DASWNJ010000000
VERSION     DASWNJ010001127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7784
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617152"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1956)
                     /locus_tag="VGB85_19005"
     CDS             complement(<1..1956)
                     /locus_tag="VGB85_19005"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HEY0136185.1"
                     /translation="MSTSIQDFNERALRGLVHTYHGDEVDHSARYVVRTGEYPVDLSG
                     RTFTTVFPYQSIHEHHELAVNPHMLTASGRLLTMDLEPLMDRNGTFIRVATRFIENQS
                     WHLRKLAPEAFVRTDFAEFSWFGTSNLTNTAPIPAFPVLGQGGYEGRRFLLSYDAGRP
                     VEVSPLTLEHLTPVGRSRDYHAAVGSSFSPMIMTSGHPVYDPDFEIGKPRLLYTNIVP
                     RVADFMSINKPIRADLYLMTWDGGPGEPTAPMRVTVDGEPVVLRHASVHQLCLTRNYL
                     VVFNANLVLNTASLLEPLTPLLWRWLIEKRTEEVPKNLRVFYRWLKIRMREATPDHTC
                     PVYFIHKDDIRKALRGEAKHVPARMIELAWEMTHALADYDDDDDKITLFCQHNIGADP
                     ADQMEEGDRLVGGGTVPESLLGMFSAATDLNQVRRHVVDLRTNTVETRAFPEADDREN
                     FPFGLNLLPPLQLAPYLSEHSSSEVPNLLRACRRMDATYWVAGGWLPHMMSTRVFANF
                     RRARRDESWPPRRLMPLSEYTERTQDPANTVRFFRLDHDMRLESAYSFPPGWFMAAPI
                     FVPRPGSTSVLDGYLIAQVWSPDSPDMEVWIWDAARPLDAGPCCTLGPGPYEKGIRPG
                     FPLHSSWVDRAGVENWVRPEYNAPTIEM"
     gene            complement(1993..3711)
                     /locus_tag="VGB85_19010"
     CDS             complement(1993..3711)
                     /locus_tag="VGB85_19010"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0136186.1"
                     /translation="MRRTKDNTREVLEMDSDPEVWRNDLASRSRGETAAEITLDNVPG
                     PGPVGRLDPSLVDGDTNAEDGLAESAVERPGYFGESARIGRFAVLRQLGEGGMGVVYS
                     AYDEELDRRVALKLLRPGRDNSPRNQARMQREARAMAKLSHPNVVQVYEVGRFEEQVF
                     VAMEFVQGRTLGVWLRAQERSWLEIIGVMIQAGRGLQAAHEAGVIHADFKPDNVLIDA
                     EDRVRVVDFGLSRRADPASASLRAAASSSTAGGSRTGTAEAGASQPAASLSLRAVPRP
                     TIDLDESGRLRPGEEPRSNARIAGTPAYMAPEQHRHHPADQRSDQFSFCVTLFTALYG
                     KHPFAGGSLLELVINLTDGKMHPPPADTQVPANVHAVVVRGLAVEREARWPSLAEMLD
                     ALEKASGRDKDPEFDLSVAKHQRVVLAGIVAASSLGMSIWVILGSAKDLMPSPQVNAM
                     AGAGINAVLFTIIFVFRGPLLKNTINRRVTAWLVVSSIAMLVHRVITMLLDVPVPTTM
                     ALDLLLLGSIAALAATTVERWIGWTATLLLTSAMLAALLPGFSSVILAIAVVGVFCLA
                     VLFWSR"
     gene            3950..5251
                     /locus_tag="VGB85_19015"
     CDS             3950..5251
                     /locus_tag="VGB85_19015"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY0136187.1"
                     /translation="MQRVLRFLLPIFVLVGAFMVARALMFAKPQVAAKPQVARELPVT
                     VLRAQPAPHPVELAALGEVKPVHRLVLQPEVSGRVIERSPSLMPGGLLHRGEPLLRVD
                     NRDHATVVAAQAAAVEQAHAALAEERTRKQVAEYEWQGSADKLSEDARSFALRETHGR
                     SVAAGLSSAKAQFEKARRDLGRTQVRVPFDALVLDASVELGQLVTPQSALATLVAIDR
                     YWVEVAVPVSQLIHFDIPGVNVGPGTRGSTAKILLDAGGGVVIERSGWIERLLGQVDS
                     RGRMARMLLVVEDPLGVKNTQTGKPMQAAPEDHAPRATLPLLLGAFVRVQIAGAPLPD
                     TTEVPRVALVDDHQVWLVVDGKLAAREVEVAWRGADTVLVRGLRPGDAVVTTPLASPT
                     EGMAVRIEGESPALVAAVPVSATPAVPATSAVPATSAVSAN"
     gene            5262..>7784
                     /locus_tag="VGB85_19020"
     CDS             5262..>7784
                     /locus_tag="VGB85_19020"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0136188.1"
                     /translation="MSGTEPENKQSLGTGPIAWMAQNPVAANLVMVFLLIGGLLMGAR
                     VKQEVFPETELDMVSISVPYPGASPAEVEQGIILAVEEAVRGIDGVKRVVATAAEGRG
                     YVTTELEVDADKEKALADIKSAVDRITSLPKEAERPIVSLLTNRSEVLSVVVYGQQDL
                     GVLRNLAEQARDQFLTDPRITQVDLAGAPPREISIEVPREQLRIHGLTLDAIAQKVTQ
                     TSLQMPGGGVKTATGEVLVRTNERREDERGFSDIPVVTGPAGTEIRLGEIAQVRKEFA
                     ETDESASFDGMPAIMIRVFRIGEQKPLEVAAAVREQSQKIQNFLPAGVKIAQWRDMSE
                     IYAQRVDLLMRNAYMGLALVMICLGLFLELRVAFWVTMGIPISFMGALMLMPTMGVSV
                     NMITLFAFIIVLGMVVDDAIVVGENIFERTQRGESYLQAAIGGAREVGVPVVFSVLTT
                     VAAFTPLFFVPGFSGKLFSVIPVIVISVLLISLFESLFILPAHLGHMKKPGETGVYGF
                     IHRQQQKVTRALERFIDRWYTPLVRGALRWRYLTLAIGVATLIITMGYVRAGLIGFRF
                     FPAIDGDVVTASIELPYGASVTETRKVQKRVLEAAQALIEEHGGAPIKRGLFAQIGSR
                     GPVMGPGGPGAQSGGGHLANVQVYFVSSDKRDFETSAFAAEWRERVGPVVEAKAVQYT
                     FNMGPSAGRPVDVEISHSDDEVLEKAAADVVAALGTQNDVTDVENGVALGKPQLDLTL
                     RAEATSLGLSATDIARQVRSAFYGSEALRQQDGRNEVRVLVRLPDAERRNENDIADLL
                     IRTPQGGEIPLREAAEIKRGRSWPSIERADGRRIIHVTADIRE"
BASE COUNT         1151 a         2654 c         2833 g         1146 t
ORIGIN      
        1 catctcgatc gtcggcgcgt tgtattccgg ccggacccag ttctcgaccc ccgcgcggtc
       61 gacccacgag gagtgcagcg ggaagccggg cctaatcccc ttttcatagg gtcccggccc
      121 gagcgtgcag cacggcccgg cgtcgagcgg acgggccgcg tcccagatcc acacttccat
      181 gtcggggctg tcgggcgacc agacctgcgc gatcagatag ccgtcgagca cgctggtcga
      241 gccgggtcgc gggacgaaga tcggggcggc catgaaccag cccggcggga acgaataggc
      301 gctctcgagg cgcatgtcgt ggtcgaggcg gaagaagcgg accgtgttcg ccgggtcctg
      361 cgtgcgctcg gtgtactcgg acaatggcat cagtcggcgc ggcggccagg actcgtcgcg
      421 gcgggcgcgg cggaagttgg cgaacacgcg cgtgctcatc atgtgcggca gccagccgcc
      481 ggcgacccag taggtcgcgt ccatgcgccg gcaggcgcgc agcaggttgg ggacctcgct
      541 gctgctgtgc tccgacaggt acggggcgag ttgcaagggc ggtaacaggt tcaggccgaa
      601 cgggaagttc tcccggtcgt cggcctcggg gaaggcgcgg gtctcgacgg tgttggtgcg
      661 caggtcgacg acgtggcggc ggacctggtt gaggtcggtc gcggcggaga acatgccgag
      721 caggctctcg ggcacggtgc caccgccgac gaggcggtcg ccttcttcca tctggtcggc
      781 cgggtcggcg ccgatgttgt gctggcagaa cagggtgatc ttgtcgtcgt cgtcgtcgta
      841 gtcggcgagc gcgtgggtca tctcccaggc gagctcgatc atgcgggcgg gcacgtgctt
      901 ggcctcgccg cgcagcgcct tacggatgtc gtccttgtga atgaaataga ccgggcaggt
      961 gtggtcgggc gtcgcctcgc gcatgcggat cttgagccag cggtaaaata cccgcaggtt
     1021 cttcgggacc tcctcggtgc gcttctcgat cagccagcgc cacagcagcg gggtcagggg
     1081 ttcgagcagg gacgccgtgt tgagcacgag gttggcgttg aagacgacga ggtaattgcg
     1141 cgtgaggcac agctggtgca ccgacgcgtg gcgcagcacg accggctcgc cgtcgacggt
     1201 gacgcgcatc ggcgcggtcg gctcgccggg gccgccgtcc caggtcatca ggtacaggtc
     1261 ggcgcggatc ggcttgttga tcgacatgaa gtcggcgacg cgcggcacga tgttggtgta
     1321 gaggaggcgc ggcttgccga tctcgaagtc ggggtcgtag accgggtggc ccgaggtcat
     1381 gatcatcggg ctgaagctgc tgccgaccgc cgcgtggtag tcgcgcgagc ggccgacggg
     1441 cgtcaggtgc tcgagcgtca gcggcgagac ctcgaccggg cggccggcgt cgtaggacag
     1501 caggaagcgg cggccctcgt agccgccctg accgagcacc gggaacgcgg ggatcggcgc
     1561 ggtgttggtc aggttcgagg tgccgaacca gctgaactcc gcgaagtccg tgcgcacgaa
     1621 ggcctccggc gcgagcttgc gcaggtgcca cgactgattc tcgatgaagc gcgtggcgac
     1681 ccggatgaag gtgccgtttc ggtccatcag cggctcgagg tccatcgtca gcaggcgacc
     1741 cgaggcggtc agcatgtgcg ggttgacggc gagctcgtgg tgctcgtgga tcgactggta
     1801 cgggaagacg gtcgtgaagg tccggcccga caggtccacc gggtactcgc ccgtgcgcac
     1861 gacgtagcgc gcgctgtggt cgacctcgtc gccgtggtag gtgtggacga gaccccgcag
     1921 ggcgcgttcg ttgaagtcct ggatgctcgt cgacatcgat gttgccgggc cgcctggtcc
     1981 tgggtccgcc ggtcagcggc tccagaacag gacggcgagg cagaatacac cgaccacggc
     2041 gatggccaag atgaccgacg agaagccggg caataaggcg gcgagcatgg cgctggtcag
     2101 caggagggtc gcggtccacc cgatccagcg ctcgacggtg gtggcggcca gcgcggcgat
     2161 cgacccgagc agcagcaggt cgagggccat cgtcgtcggc accggcacgt cgagcagcat
     2221 cgtgatcacg cggtggacca gcatggcgat gctggagaca accagccacg cggtcaccct
     2281 gcggttgatc gtgttcttga gcaggggtcc gcggaacacg aagatgatcg tgaacagcac
     2341 cgcgttgatg cccgccccgg ccatcgcgtt gacctgcggg ctcggcatca ggtccttggc
     2401 gctgccgagg atcacccaga tcgacatgcc gaggctgctc gcggcgacga tcccggcgag
     2461 cacgacgcgc tggtgcttgg cgaccgagag gtcgaactcc gggtccttgt cgcggccgga
     2521 cgccttctcg agcgcgtcga gcatctccgc cagcgagggc cagcgcgcct cacgctcgac
     2581 cgccaggccg cgcacgacca ccgcgtgcac gttggccggc acctgcgtgt ccgcgggcgg
     2641 cgggtgcatc ttgccatcgg tcaggttgat gacgagctcg agcaggctgc cgccggcgaa
     2701 cgggtgcttg ccgtagagcg cggtgaacag ggtgacgcag aagctaaact ggtcgctgcg
     2761 ctggtcggcc gggtggtggc ggtgctgctc cggggccatg taggccgggg tgccggcgat
     2821 gcgggcgttg ctgcgcggct cctcgccggg gcgcaggcgg ccggactcgt cgaggtcgat
     2881 cgtcggcctg gggaccgcgc gcagcgacag cgaggcggcc ggctgcgacg cgccggcctc
     2941 ggccgtacca gtgcgcgagc cgccggcggt gctgctgctg gcggcggcgc gcagcgaggc
     3001 gctggccggg tcggcgcggc gcgacaggcc gaagtcgacg acgcgcacgc ggtcctcggc
     3061 gtcgatcagg acgttgtcgg gcttgaagtc ggcgtggatc accccggcct cgtgggcggc
     3121 ttgcaggccg cgcccggcct ggatcatcac gccgatgatc tcgagccagc tgcgctcctg
     3181 ggcccgcagc cacacgccga gcgtccggcc ctgcacgaac tccatcgcca cgaagacctg
     3241 ctcctcgaag cgaccgacct cgtagacctg gacgacgttg gggtgcgaga gcttggccat
     3301 cgcccgcgcc tcgcgttgca tacgcgcctg gttgcgcggg ctgttgtcgc ggccgggtcg
     3361 cagcagcttg agcgcgacgc ggcggtcgag ctcctcgtcg taggccgagt agacgacgcc
     3421 catgccgccc tcgccgagct ggcgcagcac cgcgaagcgg ccgatgcggg ccgactcgcc
     3481 gaagtaaccg gggcgctcga cggccgactc ggcgaggccg tcctccgcgt tggtgtcgcc
     3541 gtcgacgagc gaggggtcga ggcgaccgac gggccccggg cccggcacgt tgtcgagtgt
     3601 gatctccgcg gcggtctcac cacgcgagcg cgacgcgagg tcgttgcgcc agacctcggg
     3661 atccgagtcc atctcgagga cctcgcgcgt gttgtccttg gtccgccgca tgccgggttc
     3721 acacccgcgc ccccgccagc cggcgcgcct gcagagatta gcgcagtggg tgccgcttgg
     3781 cgcgtccgcg agaacgcaca cccccagatc gagggcgggc gggggcccgg ggcccgggag
     3841 cccccggcct gcggtttcag gtcgcagcgg agctttccga cgcggaggcc ccgcgcagcc
     3901 ggtgtacttc ccgccgggcc cgcgctatca cccagcgcag gacgccccca tgcaacgcgt
     3961 gctgcgcttt ttgctgccga tcttcgtgct cgtcggcgcc ttcatggtcg ccagggccct
     4021 gatgttcgcc aagccgcagg tcgcggccaa gccgcaggtc gcccgcgaat tgccggtcac
     4081 cgtgctccgg gcccagcccg cgccgcaccc cgtcgagctc gccgcgctcg gcgaggtcaa
     4141 gccggtgcac cgcctcgtcc tccagccgga ggtcagcggg cgggtcatcg aacgcagccc
     4201 ctcgctgatg cccggcggtc tgctgcaccg cggcgaacct ctgctgcgcg tcgacaaccg
     4261 cgaccacgcg accgtggtcg ccgcgcaggc ggcggcggtc gagcaggccc acgcggccct
     4321 cgccgaggag cggacccgca agcaggtcgc cgagtacgag tggcagggca gcgccgacaa
     4381 gctcagcgag gacgcgcgca gcttcgcgct ccgcgagacc catgggcgca gcgtcgcggc
     4441 cggcctgagc tcggcgaagg cgcaattcga gaaggcgcgg cgggacctcg ggcgcacgca
     4501 ggtcagggtg ccgttcgacg cgctggtgct cgacgcctcg gtcgagctcg ggcaactggt
     4561 gacgccgcag agcgcgctgg cgacgctggt ggcgatcgac cgctactggg tcgaggtcgc
     4621 ggtcccggtc tcgcagctga tccacttcga catccccggg gtcaacgtgg gcccgggcac
     4681 gcggggctcg accgccaaaa ttctgctcga cgcgggcggc ggcgtcgtca tcgagcgcag
     4741 tgggtggatc gagcgcctgc tcgggcaggt cgactcccgc ggccgcatgg ccaggatgct
     4801 gctcgtggtc gaggaccccc tcggggtcaa gaacacccag accggcaagc cgatgcaggc
     4861 cgccccggaa gatcatgccc cgcgggccac cctgccgctg ctgctcggcg ccttcgtccg
     4921 cgtgcagatc gcaggggcgc cgctgccgga caccaccgag gtgccgcgcg tcgccctcgt
     4981 cgacgaccac caggtctggc tcgtcgtcga cggaaaactc gccgcgcgtg aggtcgaggt
     5041 cgcgtggcgg ggcgccgaca ccgtgctggt ccgcggcctg cgccccggcg acgccgtcgt
     5101 gacgacgccg ctcgccagcc cgaccgaggg catggccgtc cgcatcgagg gcgagtcgcc
     5161 ggcgctggtc gccgccgtgc ccgtgtccgc caccccggcc gtaccggcca cctcggccgt
     5221 accggccacc tcggccgtat cggcaaatta aaaggacact catgagcggg acggagccgg
     5281 agaacaagca gtccctgggc acggggccga tcgcctggat ggcgcagaac cccgtggccg
     5341 ccaacctggt gatggtcttc ctgttgatcg gcgggctcct gatgggcgcg cgggtcaagc
     5401 aggaggtgtt ccccgagacc gagctcgaca tggtctcgat cagcgtgccg taccccggcg
     5461 cgagcccggc ggaggtcgag cagggcatca tcctcgccgt cgaggaggcc gtgcgcggca
     5521 tcgacggcgt caagcgggtg gtcgcgaccg ccgcggaggg ccgcggctac gtcaccaccg
     5581 agctcgaggt cgacgccgac aaggagaagg cgctcgccga catcaagagc gcggtcgacc
     5641 gcatcacctc gttgcccaag gaggccgagc gcccgatcgt cagcctgctg accaaccgct
     5701 cggaggtgct gtcggtggtc gtgtacgggc agcaggacct cggggtcctg cgtaacctcg
     5761 ccgagcaggc gcgcgatcag ttcctgaccg acccgcggat tactcaggtc gacctcgccg
     5821 gcgcgccgcc gcgcgagatc tcgatcgagg tcccgcgcga gcagctgcgg atccatggcc
     5881 tgacgctcga cgcgatcgcg cagaaggtca cgcagacctc cctgcagatg cccggcggcg
     5941 gcgtgaagac ggcgaccggc gaggtcctcg tccgcaccaa cgagcgccgc gaggacgagc
     6001 gcggcttctc cgacatcccc gtcgtcaccg gcccggccgg cacggagatc cgcctcggag
     6061 agatcgccca ggtccgcaag gagttcgccg agaccgacga gtcggcgagc ttcgacggca
     6121 tgcccgcgat catgatccgg gtgtttcgca tcggcgagca aaagccgctc gaggtcgccg
     6181 ccgcggtgcg cgagcagagc cagaagatcc agaacttcct gcccgcgggc gtgaagatcg
     6241 cccagtggcg cgacatgtcc gagatctacg cccagcgcgt cgacctcctg atgcgaaacg
     6301 cctacatggg cctcgcgctg gtgatgatct gcctcggtct cttcctcgag ctgcgcgtcg
     6361 ccttctgggt gacgatgggc atcccgatct cgttcatggg cgcgctgatg ctgatgccga
     6421 cgatgggcgt gtcggtcaac atgatcacgc tgttcgcgtt catcatcgtg ctcggcatgg
     6481 tcgtcgacga cgcgatcgtc gtcggcgaaa atatcttcga gcggacccag cgcggcgagt
     6541 cctacctcca ggcggcgatc ggcggcgcgc gggaggtcgg cgtgccggtc gtgttctcgg
     6601 tgctgacgac cgtcgccgcg ttcacgccgc ttttcttcgt gcccggcttc tccggcaagc
     6661 tgtttagcgt catcccggtg atcgtcatct cggtgctgct gatctcgctg ttcgagtcgc
     6721 tgttcatcct gcccgcccac ctcgggcaca tgaagaagcc cggcgagacc ggcgtgtacg
     6781 ggtttatcca ccgccagcag caaaaggtca cccgcgcgct cgagcgcttc atcgaccgct
     6841 ggtacacgcc gctcgtgcgt ggcgccttgc gctggcgcta cctgaccctg gcgatcggcg
     6901 tggcgaccct gatcatcacg atgggctacg tgcgcgccgg cctgatcggc tttcgcttct
     6961 tcccggcgat cgacggcgac gtcgtcacag cctcgatcga gctgccctac ggggccagcg
     7021 tcaccgagac ccgcaaggtg caaaagcgcg tgctcgaggc cgcccaggcg ctgatcgaag
     7081 agcacggcgg ggcgccgatc aagcggggac tcttcgccca gatcggctcg cgcggcccgg
     7141 tgatgggccc cggcggccct ggtgcccaga gcggcggcgg gcacctggcg aacgtgcagg
     7201 tgtacttcgt gtcgagcgac aagcgcgact tcgagacctc ggccttcgcc gccgagtggc
     7261 gcgagcgcgt cggcccggtc gtcgaggcca aggcggtgca atacaccttc aacatgggcc
     7321 cgagcgccgg ccgcccggtc gacgtcgaga tcagccacag cgacgacgag gtgctcgaga
     7381 aggccgcggc cgacgtcgtc gccgccctcg gcacgcagaa cgacgtcacc gacgtcgaga
     7441 acggcgtcgc gctcggcaag ccgcagctcg acctgaccct gcgcgcggag gcgacgagcc
     7501 tcggcctcag cgcgacggac atcgcccgtc aggtgcgctc ggccttttat ggcagcgagg
     7561 cgctgcgcca gcaggacggc cgcaacgagg tgcgcgtgct ggtccgcctg ccggacgccg
     7621 agcgccgcaa cgagaacgac atcgccgacc tgctgatccg caccccgcag ggcggcgaga
     7681 tcccgctgcg cgaggccgcg gagatcaagc gcggccgcag ctggccgtcg atcgagcgcg
     7741 ccgacggccg ccgcatcatc cacgtcaccg ccgacatccg cgag
//
LOCUS       DASWNJ010001128         3692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_125652, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001128 DASWNJ010000000
VERSION     DASWNJ010001128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125652"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VGB85_19025"
     CDS             <1..792
                     /locus_tag="VGB85_19025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136189.1"
                     /translation="ACAAGDLSTSSNFGVSVTNITVDEASVGDTTDTPTSGTGIEPDA
                     STGPAEDPTTGEPATSEPATSEPATTATTGPATTGPATTGTTGDCMCDLAPDACHTAP
                     GECIDGACVYPAAPADGACDDGDPCTGSDLCDGAGACLGTPVVCEAANATGSCQDGAC
                     QFACVAPYEDCDGDWGNGCEVPVGVANQCDLDGLNPDGGCWTAYCGSSGNAKASFGTY
                     YCTDCSNCHTPGDGLWQWCNHATGNWYPAASGTCAAKDEDLVCAP"
     gene            881..1159
                     /locus_tag="VGB85_19030"
     CDS             881..1159
                     /locus_tag="VGB85_19030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136190.1"
                     /translation="MHTPRSQFLLLGGLAGLLLATACQGGTKSGVCKEYFERAEQCAA
                     RATPEKAEMLRGLAKLAREGFEDNQNKTGVEESCRDMLDQLNADPDCK"
     gene            complement(1209..1967)
                     /locus_tag="VGB85_19035"
     CDS             complement(1209..1967)
                     /locus_tag="VGB85_19035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136191.1"
                     /translation="MTAPFHCLVLTEDSGQKSFDVVEAVARKVLRLLGEGAAIDLVEV
                     EPGDERARRAMGFNGYRSTKPRDDHRRIDIAAAIVTQLLREDMPRFVIMHVDGDRRWS
                     ERGRAPGPICELQGLFERNIVRRVREGLRQKGRPELLERLLYMIPFWSIEAWLYQNST
                     ELQKIYSEDPRRHERDAELLAAWREDPSGLDEVERPKARAMTFQDGYNQRLARTFPGR
                     KVYELGQSFTYSVDEFRRCEPLFVALQALKYTAG"
     gene            complement(1964..3235)
                     /locus_tag="VGB85_19040"
     CDS             complement(1964..3235)
                     /locus_tag="VGB85_19040"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0136192.1"
                     /translation="MFKLQRIKIHQCRLVLPGTELEFKAGVNILLGKNGAGKTTLLKL
                     VSAAMGSCFVDEFGADDLHVEYAYTIGDVGVACEVRQGQAGGGGPLAADAVSPRARNF
                     YFKAEFSGPGCTLATAEIRGNRLDVTSAGSPLHSREINPMIAVVPSLDLVALIAAMMP
                     PNDLAPAIRDALGERSRVTYRLDEASDWLRQTIRDFAVIFTAIDDDERWCLPNVRHTG
                     ASEEFRAALAEHRGRFADLPEVITLHSRDLPFLERACRQIGLSDISWTLSFLEHDRRL
                     TETKRYGNSQVYVTTTGGTRLPLEKLSFGQLRLFGFFLHTMMHPHASVIDELTNGLHH
                     EMIDCCLETIGEHQAFLATQNPLLIDYVGFASAEEARRTFILCELQSAANGEKAMLWR
                     NMKEEEAREFYGDYEVGIQHANDILRKRGLW"
     gene            complement(3288..>3692)
                     /locus_tag="VGB85_19045"
     CDS             complement(3288..>3692)
                     /locus_tag="VGB85_19045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEY0136193.1"
                     /translation="DSDGHHIATLLLTFFYRYLPDLIRRGHVYIAQPPLFRVDIGKET
                     LWVADEAHLQRILAEKRGKPEIQRFKGLGEMMPETLRDTTLDPKRRQLIQVAIRDELS
                     TDRTISSLMGKDAASRYSFIMERAVEADDLDV"
BASE COUNT          631 a         1289 c         1182 g          590 t
ORIGIN      
        1 gcctgcgccg cgggcgatct ctcgacatcg agcaacttcg gcgtcagcgt caccaacatc
       61 acggtcgacg aggcgagcgt cggggacacg accgacacgc cgacctcggg cactggcatc
      121 gagcccgacg cctcgaccgg ccctgccgag gacccgacca ccggcgagcc cgcgaccagc
      181 gaacccgcga ccagcgaacc cgcgaccacc gcgaccacgg ggcccgccac gaccggcccg
      241 gcgaccaccg gcacgaccgg cgactgcatg tgcgacctcg cccccgacgc ctgccacacc
      301 gcccccggcg agtgcatcga cggcgcgtgc gtgtatccgg ccgcgcccgc cgacggcgcg
      361 tgcgacgacg gcgacccctg caccgggagc gacctgtgcg acggcgccgg cgcgtgcctg
      421 ggcacgcccg tcgtgtgcga ggccgcgaac gcgaccggca gctgccagga cggcgcctgc
      481 cagttcgcgt gcgtcgcccc gtacgaggac tgcgacggcg actggggcaa cggctgcgag
      541 gtgccggtcg gcgtcgccaa ccagtgcgac ctcgacggcc tcaacccgga cggcggctgc
      601 tggaccgcct attgcggcag cagcggcaac gccaaggcca gctttggcac ctactactgc
      661 accgactgct cgaactgcca cacccccgga gacggcctgt ggcagtggtg caaccatgcc
      721 accggcaact ggtacccggc cgcctccggc acctgcgcgg ccaaggatga agatctggtg
      781 tgcgcaccgt gagcgccgcg cctcgcgcgt gaggcgcggc tacccgcggc ccggcgaccg
      841 ggtggtcagc acgcgaggcc cgtgccatcc ttgccgcgcc atgcataccc cgcgcagcca
      901 gttcctcctt cttggtggcc tcgccggcct gctcctcgcc actgcatgtc agggcggcac
      961 gaagagcggc gtctgcaagg agtacttcga gcgcgccgag cagtgcgccg ccagggccac
     1021 ccctgagaag gccgagatgc tccgcggcct cgccaagttg gcccgcgaag gcttcgagga
     1081 caaccagaac aagaccggcg tcgaggagag ctgccgcgac atgctcgatc agctcaacgc
     1141 cgaccccgac tgtaagtagg ggctcgccct cggccgaccg gcagtcgcgc tgctccacgc
     1201 agcacgcctc agccggccgt gtacttcaac gcctgcagtg cgacgaacaa tggctcgcag
     1261 cgcctgaact cgtcgacgct gtaggtaaac gactgtccga gctcgtagac cttacgaccc
     1321 ggaaaggtcc tcgcaagtcg ctggttgtag ccgtcctgaa acgtcatggc ccgcgccttc
     1381 ggccgctcca cctcgtccag gcccgaaggg tcttctcgcc acgccgcgag cagctcggcg
     1441 tcgcgttcat ggcgccgggg atcctcgctg tagatcttct gcaactcggt ggagttctga
     1501 tacagccacg cctcgatcga ccaaaacggg atcatgtaga gcaatcgttc gaggagctcc
     1561 gggcgtccct tctggcgcag gccctcgcgt acacggcgaa cgatgttgcg ctcgaacagc
     1621 ccttgaagct cacagatcgg gcccggcgca cggccccgct cggaccagcg acggtcgccg
     1681 tcgacgtgca tgatcacgaa tcgcggcata tcctctcgca ggagctgggt gacgatcgcc
     1741 gccgcgatgt ctatccgccg atggtcatcg cgcggcttcg tgctccggta gccgttgaaa
     1801 cccatcgccc ggcgagcccg ctcatcgcca ggttcgacct cgacgaggtc gatcgccgct
     1861 ccttcaccca gcaagcgcaa caccttgcga gcgaccgctt cgacgacatc gaaggacttc
     1921 tgccccgaat cttctgtcag gaccaggcag tgaaacggcg ccgtcaccac aatccgcgtt
     1981 tcctcagaat atcgttggcg tgctggatcc cgacctcata atcgccgtag aactcccgcg
     2041 cctcttcttc cttcatgttg cgccatagca tcgccttttc gccgttcgcc gcgctctgaa
     2101 gctcgcagag gatgaaggtg cgccgggctt cctcggcgct ggcgaagccg acgtagtcga
     2161 tcaatagcgg attttgcgtc gccaggaacg cctggtgctc accgatcgtc tccagacaac
     2221 agtcgatcat ctcgtgatgc agcccgttgg tcaactcgtc gatgacgctc gcgtgtggat
     2281 gcatcatggt atgaaggaaa aaaccaaaca ggcgcagctg accgaagctc agcttttcaa
     2341 ggggtaaacg ggtacctccg gtcgtggtga catacacctg ggagttcccg tagcgcttgg
     2401 tctcggtgag ccggcggtcg tgctccaaaa aggagagggt ccaggagatg tcgctgagcc
     2461 cgatctgccg gcaagcgcgc tcgaggaacg ggaggtcgcg ggagtgcagc gtgatcacct
     2521 ccgggaggtc cgcgaaccgc ccgcggtgct ccgccagggc cgcgcggaac tcctccgaag
     2581 cgcccgtatg acggacattc ggcaggcacc agcgttcatc gtcgtcgatg gcggtgaaga
     2641 tgacggcgaa gtctcgtatc gtctgacgca gccagtcgct cgcctcgtcg agccgatatg
     2701 tgacgcggga tcgctcgccg agcgcgtcgc ggatcgccgg ggctaggtcg ttcgggggca
     2761 tcatggccgc gatcaaggcg accagatcca gcgacggcac taccgcgatc atggggttga
     2821 tctcgcgcga atgcaggggc gacccggccg acgtgacatc gaggcgattc ccgcgaattt
     2881 cggccgtggc cagagtgcag cccggtccag aaaactccgc cttgaagtaa aagttccggg
     2941 cgcgcggcga cacggcgtcg gccgcgagcg gccctccccc gccagcctga ccctgccgca
     3001 cctcgcaggc gacccccacg tcccctatcg tatatgcgta ctcgacgtgg agatcgtccg
     3061 cgccaaactc gtcgacgaag cacgagccca tcgccgccga gacgagcttg agtagtgtgg
     3121 tcttcccggc tccgttcttg cccaggagta tgttgacccc tgctttgaac tccagctccg
     3181 tgcccgggag gaccaagcgg cactggtgga tcttgattcg ctggagcttg aacatggccg
     3241 cctcgacgcc aacgatgccg ggtgccgggt gcgtccgcaa ccccaggtca gacgtcgagg
     3301 tcgtccgcct cgaccgcgcg ctccatgatg aagctgtagc gcgacgccgc atccttcccc
     3361 atcaggctgg agatcgtcct gtcggtcgac agctcgtcac gaattgcgac ctgaatgagc
     3421 tgccgccgct tggggtcgag ggtggtgtcg cgcagggtct cgggcatcat ctcgccgagc
     3481 cccttgaagc gctggatctc cggcttgccg cgcttctccg cgaggatccg ttgcaggtgc
     3541 gcctcgtcgg cgacccacag cgtctccttg ccgatgtcga cgcggaacag cggcggctgg
     3601 gcgatgtaca cgtggccgcg gcggatcagg tccggcaggt agcgatagaa gaaggtcagc
     3661 agcagcgtgg cgatgtggtg gccgtcgctg tc
//
LOCUS       DASWNJ010001129         2254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_479375, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001129 DASWNJ010000000
VERSION     DASWNJ010001129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479375"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VGB85_19050"
     CDS             <1..649
                     /locus_tag="VGB85_19050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136194.1"
                     /translation="FTFGGVPRGRYTLRAHAGGDTGAIREDVRGGVPVKLVLPPALAV
                     AGRLTGAAIPESFIVRLQGHDDEVHRQEMFSHTSGRWAFEGLAAGTYELHYEETERRG
                     FLVVALGDRPQTGLDLAIAPTGGVRGQALDAELGTPLGGIQVMVHIPGVAPKFPGWPL
                     ESMTDHEGRFEFAGMPAGPLVLNFPAYSPSSVEATAAPGSTAEVTARLRLDRNEP"
     gene            818..1390
                     /locus_tag="VGB85_19055"
     CDS             818..1390
                     /locus_tag="VGB85_19055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136195.1"
                     /translation="MDDGPVLRATAPAIAGFGAGPLVATWAALVAGLMYVLFAAEVWW
                     WAALKASLVVVGVSVVIAVVNPANRRAQASLARLRRSPVPLAGLDDWLDPACPGFGPL
                     ELELAEQHPLALRAIEVALPLRSASWTSATTLLLHPEWHGDDRDLAHLAELVTRVLAP
                     HRDELGLRQIRCHPADLVPRLTSGLDPTSP"
     gene            1471..2247
                     /locus_tag="VGB85_19060"
     CDS             1471..2247
                     /locus_tag="VGB85_19060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136196.1"
                     /translation="MYRIPEIVTAGSVLLLQAACPPQIADTTDTTTDPGSTTSAATTT
                     TDGLTSTTAPTTSASTGSTTTDITATTTGDTTTTGEDTSEGPSDPGCTFPGSTSGGAA
                     SPEVDETCACVSEASVICGDKLCPTVMGTCDRIDFDEVCDGTWKFADDALTCAIMAAA
                     AGTEGTIEWYFTANGGFGSHSGFLHIVSDRRAIRQDVEVDDLTTAVSDTELWNLDDAA
                     WFEDCLDLETFCERMDCFFAGTAGPALSLCQEGYSYYYYG"
BASE COUNT          314 a          822 c          760 g          358 t
ORIGIN      
        1 gttcaccttc ggcggtgtcc cacgcggccg gtacacgcta cgggcccacg cgggtggtga
       61 taccggggcg atccgcgagg acgtgcgcgg cggtgtgccc gtgaagctcg tgctcccgcc
      121 ggcgctcgcg gtcgccggcc gcctcacggg cgccgcgatc cccgagtcgt tcatcgtccg
      181 gttacagggt cacgacgacg aggtccatcg ccaggagatg tttagtcaca ccagcgggcg
      241 ctgggccttc gagggtctgg ccgcgggcac atacgagctg cactacgagg aaacggagcg
      301 ccgcgggttc ctcgtcgtcg cgctcggcga ccggccgcag accgggctcg acctggcgat
      361 cgcgccgacc ggtggcgtcc gcggccaggc cctcgacgcg gagctcggca cgccgctggg
      421 agggatccag gtgatggtgc acattccggg ggtggccccg aagttccccg ggtggccgct
      481 cgagagcatg accgatcacg agggtcgctt cgaattcgcc ggcatgcccg cagggccgct
      541 cgtgctcaac ttcccggcgt actcgccgag ctccgtcgag gcgaccgccg cccccgggtc
      601 gacggccgag gtcacggcgc ggctccggct cgatcgcaac gagccgtgat gtgctcgtgg
      661 gggcggtgcc gggcgttcgt ctccgtggac gtccggccgc cgcctgacta tcctcgtcat
      721 catcgatcgc tcgaaccccg actcccgcga cgctcctcga cccgggcgag gtctggcgta
      781 tcgtaccgcc gacgagcgct ctccgctgca ggccctggtg gacgacggcc ctgtccttcg
      841 tgcgaccgcc ccggcgatcg ctggcttcgg cgccggtccc ctcgtcgcaa cctgggccgc
      901 gctcgtggcg ggcctcatgt acgtgttgtt cgcggccgag gtgtggtggt gggccgcgct
      961 gaaggcgtca ctcgtcgtcg ttggcgtgag cgtggtgatc gccgtcgtca acccggcaaa
     1021 ccgacgggcg caggcgtcgc tggcccgact ccggcgctcc cctgtgccgc tcgcgggcct
     1081 cgacgactgg ctcgatccgg cgtgccccgg gttcggcccg ctcgagctcg agctcgcaga
     1141 gcagcacccg ctggccctgc gggcgatcga ggtcgcgctg cccttgcggt ccgcgtcatg
     1201 gacctccgcc acgaccttgc tgctccaccc cgagtggcac ggcgacgatc gcgacctcgc
     1261 gcacctcgcc gagctggtca cccgcgtgct cgcgccgcac cgtgacgagc tgggcctgcg
     1321 gcagatccgg tgtcacccgg cggacctcgt ccctcgcctg acgagcggcc tcgacccgac
     1381 ctcaccatag tcgaccacct gcgcatggca ggctttggcc actgctttac cgtggtcgac
     1441 ggagctcggt ggatccggca tcatgccgat atgtatcgca tccccgagat agtgaccgct
     1501 ggttcggtcc tgctgctgca ggccgcctgt ccgccgcaga tagccgacac cacggatacc
     1561 accaccgacc ccggatcgac gacgtcggcc gcgacgacca ccacggacgg tctgacgagc
     1621 accaccgcgc cgacgacgtc cgcgagcacg ggatccacga cgaccgatat caccgcgacc
     1681 acgaccggag ataccacgac gacgggtgag gacacctcgg aggggccgtc cgacccgggc
     1741 tgtacatttc cgggctcgac gtcaggtggc gccgcctcgc ccgaggtcga cgagacctgc
     1801 gcctgtgtct ccgaggcctc cgtgatctgc ggcgacaagc tctgtccgac cgtcatgggc
     1861 acctgcgaca ggatcgactt cgacgaggta tgcgacggca cctggaagtt cgccgacgac
     1921 gcgctcacct gcgcgatcat ggccgcggcc gccgggaccg agggcacgat cgagtggtac
     1981 ttcaccgcga acggcgggtt cgggagccac tccggctttt tacacatcgt cagcgaccgc
     2041 cgcgcgatcc gtcaggacgt cgaggtcgac gacctgacca ccgcggtcag cgacaccgag
     2101 ctgtggaacc tcgacgacgc cgcctggttc gaggactgcc tcgacctcga gacgttctgc
     2161 gagcgcatgg actgcttctt cgccggcact gccggccccg cgctgtccct gtgccaggag
     2221 gggtactcct attattatta cggctgacac aggc
//
LOCUS       DASWNJ010001130         3507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_269105, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001130 DASWNJ010000000
VERSION     DASWNJ010001130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269105"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="VGB85_19065"
     CDS             <1..881
                     /locus_tag="VGB85_19065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0136197.1"
                     /translation="GFLGPNGAGKTTTLKMLSGLLYPTSGEVRVAGHVPSQRRPEFLR
                     AISLVMGQKRQLSWDLPAVDTFEFNRVVFDIDARDYRRRLDELVTLLAVGEVMRRPVR
                     QLSLGERMKCELVAALLHAPRILFLDEPTIGMDVAMQLAIREFVRRYNVEHGATVILT
                     SHYMEDVAALCPRILVIDGGRLRFDGSIEALTRRIRPLRRASVRADAVPPAEVLAQLG
                     HVIENAAGRLVLDVEPARVPAVVQALLALPGAQDLAVQDAPLEEVMRELFKPTREEAR
                     EEAREEVAAAPTEPVP"
     gene            878..1687
                     /locus_tag="VGB85_19070"
     CDS             878..1687
                     /locus_tag="VGB85_19070"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEY0136198.1"
                     /translation="MSQVGAGRIFRIMGAMWRLGVSEAFAYRATMLIWILTTTFPLVS
                     LALWSSLAEHGPIGDYGRADFVAYFVAAFLIRQLTASWVVWDLAGQIASGDLSTLLMR
                     PVHPLLHHLMSNLAALPVRTMLAAPVGVIVLLVAGGVEVAAGPHLLLVPLAIGMAWLL
                     NLSVQICVACVAFWVTSSAALFEIWLGLYMVLSGYAVPTSLFPAGLAAVVRWLPFHAA
                     LGFPVELVIGRLSLAEIATGFVLQALWIAVFGGLALTLWRRGLRAYGAVGA"
     gene            1684..2487
                     /locus_tag="VGB85_19075"
     CDS             1684..2487
                     /locus_tag="VGB85_19075"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEY0136199.1"
                     /translation="MSAGRSAGYYLRLVAGQLRMSVLAALQYRLGFWTEGVLGVLWSA
                     LGMAPLLVAVEHLDTVEGWNAWELLLLTGCFTLVSGLFSALLQTALIESMNHIRKGTL
                     DYVLLRPADAMVLCLITAFAPWRLVEALGGLVLIIAALIKLGAPAPLDVLAALGIGLA
                     GLVALYALGIMVLCASFRAVQLQNLTYLMEAVLDFARWPASVFRGFLRALFTFVIPLA
                     VMTTYPAQALLGEADWSAVFTALGTSTLLMVAARVMWVRSLRGYTSASS"
     gene            complement(2509..2670)
                     /locus_tag="VGB85_19080"
     CDS             complement(2509..2670)
                     /locus_tag="VGB85_19080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136200.1"
                     /translation="MITTLLTIGVLGAVMFGMAIGVIVAGKTLRGSCGGVGTSCDCST
                     AKRAECAKH"
     gene            complement(2667..>3507)
                     /locus_tag="VGB85_19085"
     CDS             complement(2667..>3507)
                     /locus_tag="VGB85_19085"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY0136201.1"
                     /translation="WRPDSEISRFNAHASTEPFRASAGLLEVVQTASEISRASDGALD
                     VTVAPLVRAWGFGPGGRKPDAAAPTPAELEALRASVGWQRVHVDLAAGTLQKDHPALA
                     LELSAIAPGYAADRIAAGLRALGFTRTLVDVGGEILGSGTAAHGGPWRLGIERPDSAG
                     GVVHEMVRVQDLALATSGDYRNYYEQDGVRLSHTIDPRTARPIAHALASVSVLQPTAA
                     LADGWATALNVLGPEAGFALASAQDIPALFLVRVGDAFEERSTSAFAALRPDAGAATL
                     SRP"
BASE COUNT          417 a         1208 c         1320 g          562 t
ORIGIN      
        1 tcggcttcct cgggcccaac ggcgccggca agacgacgac gctgaagatg ttgtccggac
       61 tgctttatcc gacctcgggc gaggtgcggg tcgccggtca cgtgccgtcg cagcgccgcc
      121 cggagttcct gcgcgcgatc agcctggtga tgggccaaaa gcgccagctg agctgggatc
      181 tgccggcggt cgacaccttc gagttcaacc gcgtggtgtt cgacatcgac gcccgcgact
      241 accggcgtcg cctcgacgag ctggtgacgc tgctcgcggt cggcgaggtg atgcgcaggc
      301 cggtgcgcca gctcagcctc ggcgagcgca tgaagtgcga gctcgtcgcg gccctgctgc
      361 acgcgccgcg gatcctgttc ctcgacgagc cgacgatcgg catggacgtc gcgatgcagc
      421 tggcgatccg cgagttcgtg cgccgctaca acgtcgagca cggggcgacc gtgatcctga
      481 cgagccacta catggaggac gtcgcggccc tgtgcccgcg gatcttggtg atcgacggcg
      541 gccgcctgcg cttcgacggc agcatcgagg ccctgacgcg caggatccgc ccgctgcgcc
      601 gcgccagcgt ccgcgccgac gctgtgccgc ccgccgaggt gctcgcgcag ctcggccacg
      661 tgatcgagaa cgcggccgga cgcctcgtcc tcgacgtcga gccggcgcgc gtgcccgcgg
      721 tcgtgcaggc gctgctggcc ctcccgggcg cgcaggacct cgcggtccag gacgcgccgc
      781 tcgaggaggt gatgcgcgag ctcttcaaac cgacccgcga ggaggcccgc gaggaggccc
      841 gcgaggaggt cgccgcggcg ccgacggagc ctgtcccatg agccaggtgg gtgcggggcg
      901 gatcttcagg atcatgggcg cgatgtggcg cctcggcgtg tcggaggcgt tcgcgtaccg
      961 ggccacgatg ctgatctgga tcctgacgac gaccttcccg ctggtctcgc tggcgctgtg
     1021 gagcagcctc gcggagcacg ggccgatcgg cgactacggg cgcgccgact tcgtcgcgta
     1081 cttcgtcgcc gcgttcctga tccgccagct gacggccagc tgggtggtct gggacctcgc
     1141 cggccagatc gccagcggtg acctgagcac gctgctgatg cgcccggtcc acccgctgct
     1201 gcaccacctg atgtccaacc tcgccgcgct gccggtgcgc acgatgctcg cggcgccggt
     1261 cggcgtgatc gtgctgctcg tcgcgggtgg ggtcgaggtc gccgccggcc cgcacctgct
     1321 gctcgtcccg ctggcgatcg gcatggcctg gctgctcaac ctcagcgtgc agatctgcgt
     1381 cgcctgcgtg gccttctggg tgacctcgtc ggcggcgctg ttcgagatct ggctcggcct
     1441 ctacatggtg ctctcgggct acgcggtgcc gaccagcctg ttccccgcgg gcctcgccgc
     1501 ggtcgtccgc tggctgccgt tccacgccgc gctcggcttt ccggtcgagc ttgtgatcgg
     1561 gcgcctgtcg ctcgccgaga tcgcgaccgg cttcgtcttg caagcgctgt ggatcgcggt
     1621 cttcggcggt ctcgcgctga cgctgtggcg ccggggcctg cgcgcgtacg gggcggtggg
     1681 cgcgtgagcg cgggacgcag cgccggctat tacctgcggt tggtggccgg ccagctgcgc
     1741 atgagcgtgc tcgcggccct gcagtaccgc ctcggcttct ggaccgaggg cgtgctcggg
     1801 gtcctgtgga gcgcgctcgg catggcgccg ttgctggtcg cggtcgaaca cctcgatacc
     1861 gtcgagggct ggaacgcgtg ggagctgctg ctgctgaccg gctgcttcac gctggtctcg
     1921 gggctgttca gcgcgctgct gcagacggcg ctgatcgaga gcatgaatca catccgcaag
     1981 ggcacgctcg actacgtgct gctgcgaccc gccgacgcga tggtcctgtg cctgatcacc
     2041 gcgttcgcgc cgtggcgcct cgtcgaggcg ctcggcggcc tggtgctgat catcgcggcg
     2101 ctgatcaagc tcggcgcccc cgcacccctc gacgtgctcg cggccctcgg catcggcctc
     2161 gccgggctcg tcgccctcta cgcgctcggc atcatggtcc tgtgcgcgag ctttcgcgcg
     2221 gtccagctgc agaacttgac ctacctgatg gaagcggtgc tggacttcgc ccgctggccc
     2281 gcgagcgtgt tccgcgggtt cttgcgggcg ctgttcacgt tcgtgatccc gctggcggtg
     2341 atgacgacgt acccggcgca ggcgctgctc ggggaggcgg actggtccgc cgtgttcacg
     2401 gcgctcggga cgagcacgct gctgatggtc gcggcccggg tgatgtgggt gcgatcgttg
     2461 cgggggtata cctcggcctc gagttgaggc cggaagatct gtccacgctc agtgcttggc
     2521 gcactcggcc cgcttggcgg tcgagcagtc gcagctggta ccgacgccgc cgcaggagcc
     2581 gcgcagggtc ttgccggcga cgatgactcc gatggccatg ccgaacatga cagcgccgag
     2641 gacgccgatg gtgagcaggg tggtgatcat ggccggctca gcgtggccgc tccggcgtcg
     2701 ggacgcaagg cggcgaaggc cgacgtgctg cgctcctcga aggcgtcgcc gacgcgcacg
     2761 aggaacagcg cagggatgtc ctgcgcgctc gccagcgcga agccggcctc cggaccgagg
     2821 acgttgagcg cggtggccca gccgtccgcg agcgctgcgg tcggctggag aacgctcacg
     2881 gaggcgagcg cgtgggcgat gggccgggcc gtgcgcgggt cgatcgtgtg cgacaggcgg
     2941 acgccgtcct gctcgtagta gttgcggtaa tcgccggagg tggcgagcgc gaggtcctgc
     3001 acgcgcacca tctcgtggac gacgccgccg gcgctgtcgg gccgctcgat gccgaggcgc
     3061 cacgggccgc cgtgcgcggc ggtgccggac ccgaggatct cgccgccgac gtcgacgagc
     3121 gtgcgcgtga agccgagcgc ccgcagaccg gcggcgatgc ggtcggcggc gtagcccggg
     3181 gcgatcgcgg acagctcgag cgcgagggca gggtggtcct tttgcaaggt gccggcggcg
     3241 aggtcgacgt gcacgcgctg ccagccgacg ctggcccgca gcgcctcgag ctcggcgggc
     3301 gtcggcgcgg ccgcgtcggg cttgcggccg ccgggaccga agccccacgc gcgcaccagc
     3361 ggcgcgacgg tgacgtcgag cgcgccgtcg ctggcgcggc tgatctcgct cgcggtctgc
     3421 acgacctcga gcagcccggc cgaggcccgg aacggctcgg tgcttgcgtg cgcgttgaag
     3481 cggctgatct ccgagtcggg gcgccag
//
LOCUS       DASWNJ010001131         1950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_231781, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001131 DASWNJ010000000
VERSION     DASWNJ010001131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231781"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VGB85_19090"
     CDS             <1..202
                     /locus_tag="VGB85_19090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136202.1"
                     /translation="IRALHGVEELQRELGHLVVEARMPQIGQVPSGTYSGDGWVIIRH
                     PDTAVVYAAIQQIITRLRVEVG"
     gene            complement(203..1264)
                     /locus_tag="VGB85_19095"
     CDS             complement(203..1264)
                     /locus_tag="VGB85_19095"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY0136203.1"
                     /translation="MQALVVTPGQPDSARLAQMDEPSPDEGAVLVEMVAVGVCGTDRE
                     ILHGDYGEGPGGEQALVLGHESLGRVLEAPAGSGLASGDLVVGMVRHPDPVPCVNCAV
                     GEWDMCRNGQFTEHGIKGRHGFCRERFRLEPGRLIRVEPALGLRGVLLEPTSVVAKAW
                     EHILRIGGRARFAPRRALITGGGPIGLLAALIAVQHGLEVHLLDHAQEGLKPALTREL
                     GATYHGDPGELAGQTFDVIVECTGAPQLVLGVMSWSGPDGIVCLTGVSSGGRKLPVDL
                     GDLARGIVLENTAIFGSVNANRRHYEQAATALARADQGWLGRLITRRVPMARWSEALE
                     RDKGDVKVVILGPGADGLT"
     gene            1316..>1950
                     /locus_tag="VGB85_19100"
     CDS             1316..>1950
                     /locus_tag="VGB85_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY0136204.1"
                     /translation="MSASDLPDLRRAREGAVLLLTLDRPDARNAYSEAMVDSLVRALD
                     EADRDDEVRCIILTGAGAAFSAGGDLKRMLNHEGMFAGGPVELRRRYIDGIQRIPRRF
                     ALAEKPVIAAINGPAIGAGLDLACMCDLRIAARGAQLGSTFVKVGLVPGDGGAYFLSR
                     TVGFSRALELMLTGRLVDTDEALRIGLVHQSVEPAELLPTARALAEQLAALP"
BASE COUNT          262 a          776 c          650 g          262 t
ORIGIN      
        1 gatccgcgcc ctgcacggcg tcgaggagtt gcaaagggag ctcggccacc tcgtcgtcga
       61 ggccaggatg ccgcagatcg gtcaggtgcc ctcggggacg tactcgggcg acggctgggt
      121 gatcatccgc cacccggaca ccgccgtcgt gtacgccgcg atccagcaga tcatcacgcg
      181 cctgcgggtc gaggtcggct gatcaggtca ggccgtccgc gccgggcccg aggatcacga
      241 ccttgacgtc gcccttgtcg cgctcgagcg cctccgacca gcgagccatc ggcacgcgcc
      301 gggtgatcag gcgcccgagc cagccctggt ccgcgcgcgc cagcgcggtc gcggcctgct
      361 cgtagtgccg gcggttggcg ttgaccgacc cgaagatcgc ggtgttctcg agcacgatcc
      421 cccgcgcgag gtcaccgagg tcgaccggca atttgcgacc cccgctcgag acccccgtca
      481 gacacacgat cccatccggc ccgctccacg acatcacgcc tagcacgagc tgcggcgccc
      541 cggtgcactc gacgatgaca tcgaaggtct gccccgcgag ctcgccgggg tcgccgtggt
      601 aggtcgcgcc gagctcgcgc gtgagcgccg gcttgaggcc ctcctgcgcg tggtcgagaa
      661 ggtggacctc gaggccgtgc tggactgcga tcagcgccgc gagcaggccg atcggcccgc
      721 cgccggtgat cagcgcccgc cgcggcgcga accgggcccg cccgccgatc cgcaggatgt
      781 gctcccacgc cttcgccacc acgctggtcg gctcgagcag cacgccacgc aggccgagcg
      841 ccggctcgac gcggatcagc cgcccgggct cgaggcggaa gcgttcgcga cagaacccgt
      901 ggcggccttt gatcccgtgc tcggtgaatt gcccgttgcg gcacatgtcc cactcgccca
      961 ctgcgcagtt tacgcaaggc accgggtccg ggtgccgcac catgccgacg acgaggtcgc
     1021 ccgacgccag gccgctcccg gcgggcgcct cgagcacgcg cccgagcgac tcgtggccga
     1081 gcaccagcgc ctgctccccg cccggcccct cgccgtagtc tccgtgcaag atctcccggt
     1141 ccgtgccgca cacgccgacc gcgaccatct cgacgagcac cgcgccctcg tcgggcgacg
     1201 gctcgtccat ctgcgcgagc cgggccgagt cgggctgacc aggggtgacc accagcgcct
     1261 gcatcccatg catgtagcag ggcccgaccg gcgtgcatgc ggtagcctcg cgcgcatgtc
     1321 cgccagcgac ctcccggacc tccggcgcgc ccgcgagggc gccgtcctcc tgctgaccct
     1381 cgaccgcccc gacgcccgca acgcgtacag cgaggcgatg gtcgacagcc tggtgcgcgc
     1441 gctcgacgag gccgaccgcg acgacgaggt ccgctgcatc atcctcaccg gcgccggcgc
     1501 ggcgttctcg gccggcggcg acctcaagcg catgctcaac cacgagggca tgttcgccgg
     1561 cggtccggtc gagctgcgcc gccgctacat cgacggcatc cagcgcatcc ctcgccgctt
     1621 cgcgctcgcc gagaagccgg tgatcgccgc gatcaacggc ccggcgatcg gcgccggcct
     1681 cgacctcgcg tgcatgtgcg acctgcggat cgccgcccgc ggcgcccagc tcgggtcgac
     1741 cttcgtcaag gtcggcctcg tgcccggcga cggcggggcc tattttctga gccgcaccgt
     1801 cggcttctcc cgcgccctcg agctgatgct gaccggccgc ctcgtcgaca ccgacgaggc
     1861 cctgcggatc ggcctcgtgc atcagtcggt cgagcccgcc gagctgctgc cgaccgcccg
     1921 cgccctcgcc gaacagctcg ccgccctccc
//
LOCUS       DASWNJ010001132         5594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_304188, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001132 DASWNJ010000000
VERSION     DASWNJ010001132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304188"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VGB85_19105"
     CDS             <1..956
                     /locus_tag="VGB85_19105"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY0136205.1"
                     /translation="FPDDPRVYVNYNPQDWQGPGPTYIDEYKLDPNDPNKVDPGSRRL
                     VYAVGQPASNHNGGMISFGPDGMLYIGMGDGGGSNDQFDTGRDPSSQHAKILRIDVEP
                     DGTADSNKACGNCPMVDGFDFTVPADNPHVGDDAYAPEVYASGMRNPWRFSFDTATGV
                     LYVADVGQNQYEEVSIVKAGGDYGWSDMEGNHCFGGANCDDSAAPNAENADGLIAPIA
                     EFSHSDNNRCSITGADVYHSCQVPGWDGVYFYADYCSSEIFALKWDGSNVEQMNVVAN
                     TNDERIIGSGRNGYGDVYINTVVVTGLNTLVDGKVYRIVPE"
     gene            953..1432
                     /gene="ybaK"
                     /locus_tag="VGB85_19110"
     CDS             953..1432
                     /gene="ybaK"
                     /locus_tag="VGB85_19110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEY0136206.1"
                     /translation="MTTKTNAARILDNLGIAYELRSYAVDLEDLTAVSVAAKVGLPPE
                     QVFKTLVARGDRHGVCLALVAGDDQLDLKALARATGDRRCELVALKEVLPLTGYVRGG
                     VTALACKKDYPVLIDETAQLHTTISVSAGARGLQILLAPEDYARAVRGRFVALTGPG"
     gene            1499..2431
                     /locus_tag="VGB85_19115"
     CDS             1499..2431
                     /locus_tag="VGB85_19115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136207.1"
                     /translation="MIVVLFLLCAPAPDLAHDDMRELAVALSDEGRFVEAAVRFEAVA
                     AAGGPPIARFEAGQMRFVAGHMAHAARHFQAYIASGLDADELSLAQSRLAKAATGTRL
                     IELRLSPAITTTLLAHRVADPPGSQRPDLDSPVLGGLAALRLDPGTWELRLDVPGYLP
                     LRHTIEVTDARTPVELRLVPAPSTAPAPTIDPSPRTHDLRRARAETIAGAVTLPLGAA
                     ALAGFITSTVAYRGSVAREKSVGYQCNDRGALDDLRVTARRQVGAMVGLGVASGALLT
                     AGVVLLVRGAALRRAHLTLDLHPDHAGLLLSGRF"
     gene            2443..3336
                     /locus_tag="VGB85_19120"
     CDS             2443..3336
                     /locus_tag="VGB85_19120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136208.1"
                     /translation="MRLTCTLVPLLACGGCLLRDIDRHLDELHCSGTTSTDTTTSDPD
                     TTACPDSTGGSTTTTSTTDTTGDTTSTDSDSAGTGTSTGTSTTDTGSSTGEPVAVCGN
                     GVIEAFGPTPEDCDDGNDDPDDGCSDCGRDRLVFITSADYQGAIFMGIVGVDQRCRNL
                     AAIQNLPKFAEFKAWISDSTISAKQRMFRGRGRYVLRNGLVVAQSWDALLAGELKNPI
                     NVTETSETKNYGAWTGTMPDGSAAVGSDHCADWSSQLDKNSGFWGRSAMVTAEWTIAD
                     PPVDQPGDCAVARALYCFEQE"
     gene            3458..4474
                     /locus_tag="VGB85_19125"
     CDS             3458..4474
                     /locus_tag="VGB85_19125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136209.1"
                     /translation="MTSESSGPTGEPPVPTTGDGPVITSTDGPSSTTTETGDGSTDDT
                     STDDTSTEPAGTPVCGDGVVEGEEICDEGFAANTLHGACLPGCVPARCGDGFVQAGVE
                     ECDMGDEVNELKYGGCVPITCKWSARCGDGQVDAPNEVCDPGDPASQGEELVGCDAAC
                     RFKGRIVFISSVTYDGNLGGLAGADSLCRDLAAGFDASHADDYVAWLGDAKLSPKDRI
                     QHGPEFDATPYVLRSGVQVAANFKDLIDNGPWPGIYLTDTDETLIDAYVWTNTAIDGS
                     LFSAEDHCEGWGSNSGNHSARVGRNALPSDSPELDTWKQLGHWTSRKPLTCEFKHHLY
                     CFEN"
     gene            4642..5415
                     /locus_tag="VGB85_19130"
     CDS             4642..5415
                     /locus_tag="VGB85_19130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136210.1"
                     /translation="MLTRSPRSAACLLLLSAATLACPSNESFESLGDTPATTTATTGG
                     SDPATSSTGAPDTEPTSSSTGGTSTTDSSTTDTSTTDVDTTTDGTTGTTGEPSNLCPD
                     HMATDACCCFETHMGFSVSNVCGTESLCALAQFECNQNDDTCTAVDEVVVDCVLEALR
                     DGTMAGELKVDYNIDQGFGRRKIDLYLQGDGSAYVVDANEEDLSGVSRATGRHLLREP
                     AYFEDCLMGDAQIKADCLENVLGGKATEVCTDEYQYDVN"
     gene            complement(5412..>5594)
                     /locus_tag="VGB85_19135"
     CDS             complement(5412..>5594)
                     /locus_tag="VGB85_19135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136211.1"
                     /translation="TGESPAVTSTGAEVTSASSGSTGAADTGAQGEGGGCGCRGSGGA
                     GLLWAPLLVLLRRRRA"
BASE COUNT          949 a         2157 c         1706 g          782 t
ORIGIN      
        1 acttccccga cgacccgcgc gtctacgtca attacaaccc gcaggactgg cagggccccg
       61 gcccgaccta catcgacgag tacaagctcg accccaacga cccgaacaag gtcgaccccg
      121 gctcgcgccg cctcgtctac gccgtcggtc agccggcctc caaccataac ggcggcatga
      181 tcagcttcgg cccggacggc atgctgtaca tcggcatggg cgacggcggc ggctcgaacg
      241 accagttcga caccggccgc gacccctcct cccagcacgc caagatcctt cgcatcgacg
      301 tcgagcccga cggcaccgcc gacagcaaca aggcgtgcgg caactgcccg atggtcgacg
      361 gcttcgactt caccgtgccc gccgacaacc cccacgtcgg cgacgacgcc tacgcccccg
      421 aggtctacgc ctccggcatg cgcaacccgt ggcgcttcag cttcgacacc gcgaccggcg
      481 tgctctacgt cgccgacgtc gggcaaaatc agtacgagga ggtctcgatc gtcaaggccg
      541 gcggcgacta cggctggagc gacatggagg gcaaccactg cttcggcggc gccaactgtg
      601 acgactccgc ggcgcccaac gccgagaacg cggacggcct gatcgccccg atcgccgagt
      661 tctcgcacag cgacaacaac cgctgctcga tcaccggcgc ggacgtctat cactcgtgcc
      721 aggtcccggg ctgggacggc gtgtacttct acgccgacta ctgctcctcc gagatcttcg
      781 cgctcaagtg ggacggcagc aacgtcgagc agatgaacgt cgtcgccaac accaacgacg
      841 agcgcatcat cggctccggt cgcaacggat acggcgacgt gtacatcaac accgtcgtcg
      901 tcaccggcct caacaccctc gtcgacggca aggtctaccg catcgtcccc gagtgaccac
      961 caagaccaac gccgcccgca tcctcgacaa cctcgggatc gcctacgagc tacgcagcta
     1021 cgcggtcgac ctcgaggacc tcaccgcggt cagcgtcgcc gccaaggtcg gcctcccgcc
     1081 cgagcaagtt tttaaaacgc tcgtcgcgcg aggcgaccgt catggcgtct gcctcgccct
     1141 cgtcgccggt gacgaccagc tcgacctcaa ggccctcgcc cgggcgaccg gcgaccgccg
     1201 atgtgagctg gtcgcgttga aagaggtcct gccgctgacc ggttacgtcc gcggcggcgt
     1261 cacggccctc gcgtgcaaga aggactaccc ggtgctgatc gacgagacgg cgcagctgca
     1321 caccacgatc tccgtgtcgg ccggcgcgcg tggcctgcag atcttgctcg cccccgagga
     1381 ctacgcccgc gcggttcgcg ggcgcttcgt ggccctcacc ggccccggct gagctcgtgt
     1441 tatacgggcg aagacgcgcc tcccgcgtgc cgcccgacaa ggagtcgtcc gagtgcccat
     1501 gatcgtcgtt ctgttcctcc tgtgcgcacc ggcgccggac ctcgcccacg acgacatgcg
     1561 cgagctggcg gtcgcgctct ccgacgaggg ccgcttcgtc gaggccgcgg tccgcttcga
     1621 ggcggtcgcc gcggccggcg gcccaccgat cgcccgcttc gaggccggcc agatgcgctt
     1681 cgtcgccggt cacatggccc acgcggcgcg tcacttccag gcctacatcg ccagcggcct
     1741 cgacgcggac gagctgtccc tcgcccagtc ccggctcgcc aaagccgcca cgggcacgcg
     1801 cctgatcgag ctgcggcttt cgccggcgat caccacgacc ctgctcgccc accgcgtcgc
     1861 agatcccccc ggctcgcagc gccccgacct cgacagcccg gtcctcggag gcctcgccgc
     1921 gctgcgcctc gaccccggga cctgggagct gcgcctcgac gtcccggggt acctgccgct
     1981 gcgccacacc atcgaggtca ccgacgctcg cacgcccgtc gagctgcgac tcgtgcccgc
     2041 gccctccacg gcccccgcgc cgacgatcga cccgagcccg cggacccacg acctgcgtcg
     2101 cgctcgcgcc gagacgatcg ccggcgccgt gaccctgccg ttgggcgcgg cggccctcgc
     2161 cggcttcatc acgtcgaccg tcgcctaccg cggcagcgtc gcccgtgaaa agagcgtcgg
     2221 ctatcagtgc aacgatcgcg gcgccctgga cgacctccgt gtcaccgcgc ggcgacaggt
     2281 cggcgcgatg gtcggcctcg gcgtcgcctc cggggcgctg ctgaccgccg gcgtggtcct
     2341 cctcgtccgc ggcgctgccc tccgccgcgc ccacctcacc ctcgacctcc accccgacca
     2401 cgccggcctc ctgctgtccg gccgcttctg atgcgagacc ccatgcgcct gacctgcacc
     2461 ctcgtccccc tcctcgcctg cggcggctgc ctgctccgcg acatcgaccg ccacctcgac
     2521 gagctgcact gcagcggtac caccagcacg gacacgacca cgtccgaccc cgacacgaca
     2581 gcttgcccgg actcgaccgg cggcagcacg accacgacct cgacgaccga cacgacgggc
     2641 gacaccacca gcaccgactc ggacagcgcc ggcaccggca ccagcactgg caccagcacc
     2701 accgacaccg gcagctccac cggcgagccg gtcgccgtgt gcggcaacgg ggtcatcgag
     2761 gcattcgggc ccacccccga ggactgtgac gacggcaacg acgacccgga cgacggctgc
     2821 agcgactgcg gccgcgaccg cctggtgttc ataacctcgg ccgactacca gggcgcgatc
     2881 ttcatgggca tcgtcggcgt cgaccagcgc tgccgcaacc tggccgccat ccagaactta
     2941 ccgaagttcg ccgagttcaa ggcgtggatc agcgactcga cgatctccgc gaagcaacgg
     3001 atgttccgcg gccgcggccg ctacgtcctg cgcaacggcc tggtggtcgc ccagagctgg
     3061 gacgccctcc tggcgggtga gctcaagaac ccgatcaacg tcaccgagac cagcgagacc
     3121 aagaattacg gcgcgtggac cgggacgatg ccggacggca gcgccgcggt cggctcggac
     3181 cactgcgccg actggagcag ccagctcgac aaaaactcag gtttttgggg gcgtagcgca
     3241 atggttactg ccgagtggac gatcgcagat ccgccggtcg atcaacccgg tgactgcgct
     3301 gtcgcacgcg cgctgtactg cttcgaacaa gaataacgac aagtgaaggc acacgatgag
     3361 ccgcaaatgc tggatatcaa aagttttatt ggctgcgctc ggttgctgcg ttccacccgg
     3421 atgcacgcac gagggcgatg ctgacgctcc ggagccgatg acctcggagt catcggggcc
     3481 caccggcgaa cctccggtgc cgacgaccgg cgatggcccg gtcatcacca gcacggacgg
     3541 accgtcctcg accaccaccg agaccggcga cggatcgacc gacgacacct cgaccgacga
     3601 cacctcgacc gaacctgccg gcacgcctgt ctgcggcgac ggcgtggtcg agggcgagga
     3661 aatttgcgac gagggcttcg ccgccaacac gttacacggc gcctgcctcc ctggctgtgt
     3721 tcctgcccgc tgtggcgacg gcttcgtgca ggccggggtc gaggagtgcg atatggggga
     3781 cgaggtcaac gagctaaagt atggaggttg cgtcccgatc acctgcaaat ggtcggctcg
     3841 ctgtggtgat ggtcaggtcg acgcgccgaa cgaggtctgc gatcccggcg accccgccag
     3901 ccagggcgag gagctggtcg gctgtgacgc ggcctgccgc ttcaagggtc ggatcgtctt
     3961 catttcctcc gtcacgtacg atggcaatct cggcggcctc gctggcgccg attcgctgtg
     4021 tcgtgacctc gccgcaggct tcgacgcctc gcacgcggac gactacgtcg cctggctcgg
     4081 cgatgcgaag ctgtcgccca aagaccgcat ccagcacggc cccgagttcg acgcgacccc
     4141 gtacgtgctt cgcagcggcg tccaggtggc cgccaacttc aaggacctga tcgacaacgg
     4201 accgtggccc ggcatctacc tcacggacac agacgagacg ctcatcgatg cttacgtgtg
     4261 gaccaacacc gcgatcgacg gcagcctctt cagcgccgag gatcactgtg agggctgggg
     4321 ctccaattcc ggcaatcaca gcgcacgcgt cggcaggaac gcgctcccat cggactcgcc
     4381 cgagctcgac acctggaagc agctcggcca ctggaccagt cgaaagccgc tgacctgtga
     4441 attcaagcac cacctctact gcttcgagaa ctgaccgacg gggcggtgca taggcccagc
     4501 agccacccga ccccagccgc gccgatgccc ggcgtctgca ccacgctcgt gaccggcgct
     4561 ctgcctgcgc cgttcgcggg cgcctttgtc ccactcgccg ggccgtagtc gaccgccgcg
     4621 ataagagcga acatgcgccg catgcttacc cgttctcccc gctccgccgc ctgcctgctc
     4681 ctgctgagcg cggccaccct cgcctgtccg agcaacgagt ccttcgaatc gctcggcgac
     4741 actcccgcga ccaccaccgc gaccaccggc ggcagtgacc ccgccaccag ctcgaccggc
     4801 gcgccggaca cggagccgac ctcgagctcg accggcggca cctcgaccac cgacagctcg
     4861 accaccgaca cctcgaccac cgacgtcgac accaccaccg acggcactac cggcaccacc
     4921 ggcgagccct ccaacttgtg ccccgaccac atggccaccg acgcctgctg ctgcttcgag
     4981 acacacatgg gctttagcgt cagcaacgtc tgcggcaccg agagcctgtg cgccctcgcg
     5041 cagttcgagt gcaaccagaa cgacgacacc tgcacggccg tcgacgaggt cgtggtcgac
     5101 tgcgtgctcg aggcgctgcg cgacggcacg atggccggcg agctcaaggt ggattacaac
     5161 atcgaccagg gctttggtcg ccgcaagatc gacctgtacc tccagggtga cggcagcgcg
     5221 tacgtcgtcg acgccaacga ggaggacctg tccggcgtct ccagggcgac cggccgccac
     5281 ctgctgcgcg agccggccta cttcgaggac tgcctgatgg gcgacgccca gatcaaggcg
     5341 gactgcctcg agaacgtgct cggcggcaag gccaccgagg tctgcaccga cgagtaccag
     5401 tacgacgtca actaagcccg cctccggcgt aacaacacca gcagcggcgc ccacagcagc
     5461 ccggcgccgc cgctgccgcg acaaccacaa ccgccgccct cgccctgcgc gccggtgtcg
     5521 gccgcgcccg tcgagcccga cgaggcgctc gtgacctccg cccccgtgct cgtcaccgcc
     5581 gggctctcac ccgt
//
LOCUS       DASWNJ010001133         1535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_656884, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001133 DASWNJ010000000
VERSION     DASWNJ010001133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656884"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(809..>1535)
                     /locus_tag="VGB85_19140"
     CDS             complement(809..>1535)
                     /locus_tag="VGB85_19140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136212.1"
                     /translation="DLLAVQEIHDPAALQALLPGWQLAISEHGGRGQQRLGVAFDPIA
                     LAQVGPGREHAELSMRGHVRPGFSVYLRAHGGGPDFHVLVVHLKAMRDGHSLRVQQWG
                     LLAELAGDLLRRDPDLVVLGDFNVTGPAGGSPADELAALEARLGPLGLRRVTGPESCS
                     AYWDGTRRDAHKEPSLLDLVWVAGLTESHSAAAQARPLHHCARHRCRPFRSTEAHPEP
                     DYADLSDHCPVVVDLAAGLDDDP"
BASE COUNT          246 a          476 c          569 g          244 t
ORIGIN      
        1 tgccgccggc gagcaggcca aaggtacgga aattggtggt gtgcatgagt cgtcttttcc
       61 tgaggtttgc gggctacgaa cagctcgtcg gggtctgggc cgggaggatc gctcgagtgc
      121 ggtccaaccg caaatgatca aaaatgccca cgagtgcccc taacatggtt atttcgaacg
      181 tggcggggtt tggagacagg cttgtaagcc tgtccccgga cagggtcgca aaggtcccgc
      241 cactgacatt tcagcgacgg tcggattcct gcgcagagcg cggacgatcg cagatacgcg
      301 gaggccgacg gcggccgtgt cgacagtgat gtgggtcgag tcaacgccac tgaagtcgac
      361 gtcactgaag tggatgacgt cgacgagggc gtgcgtgcag cagaggacgt caaagggcgc
      421 cgtggcctgc ttcgtcgcgg gccgtgcctt cgagtggtgc gcgggtcgtc tcaagtcgtt
      481 ctctgaaggg ggtgagcgca gccgtgcagg ccgtcgacgg aggtcgaggc tgctcgcttc
      541 atcactttcg tcattccgaa caaagcgcgc atgaacccgg gcgggttagc tatgcgagct
      601 tcgatggaat gacgagctgg catggatccg ggcgagcttg gcttggacag cacagcccgg
      661 caaatcttca gcagtccggc gcccccaatg ttcggcgctg ctttgcatca cccgaagggc
      721 gctggtgggc cggccggggc cgctgtgcca cttacctgtc ccttggggca tgcccgaaag
      781 gccattttaa cgctggctcg gcgtcaactc acgggtcgtc gtcgaggccg gcggcgaggt
      841 cgacgacgac cgggcagtgg tcgctgaggt cggcatagtc gggctccggg tgggcctcgg
      901 tcgagcggaa cggccggcag cggtggcggg cgcagtggtg cagcgggcgg gcctgggcgg
      961 cggcgctgtg gctctcggtc aggccggcga cccacacgag gtcgagcagg gagggctcct
     1021 tgtgggcgtc gcggcgggtc ccgtcccagt atgcggagca cgactcgggg ccggtcacgc
     1081 gacgcagccc gagcggcccg aggcgcgctt ccagggccgc gagctcgtcc gcagggctgc
     1141 cgccggcggg tccggtgacg ttgaaatccc cgagcacgac gaggtccgga tcacggcgca
     1201 gcagatctcc cgcgagctcg gcgagcagcc cccattgctg gacccgcagc gagtgaccgt
     1261 cgcgcatcgc cttgaggtgc acgacgagca catggaagtc gggcccgccg ccgtgagcgc
     1321 gcaggtagac ggaaaaaccc ggacgcacgt ggccgcgcat gctcagctcg gcgtgctcgc
     1381 gcccgggtcc gacctgcgcg agcgcgatcg ggtcgaaggc gacgccgagc cgctgctggc
     1441 cgcgcccgcc gtgctcgctg atcgccagct gccagcccgg cagcaacgcc tgcagcgccg
     1501 ctggatcgtg gatctcctgg accgcgagca gatcg
//
LOCUS       DASWNJ010001134         1771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_125722, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001134 DASWNJ010000000
VERSION     DASWNJ010001134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125722"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VGB85_19145"
     CDS             complement(<1..356)
                     /locus_tag="VGB85_19145"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0136213.1"
                     /translation="MIVLGGGTMGLASAWALARRGARVTVLERFGHVHTQGSHGGHTR
                     IIRESYHEGAGYVPIVREAARLWEGLSRRAGEPLLVRTGMLEIGAPNDPFYKNTIAAN
                     VASEVEFTEVEADEARR"
     gene            complement(374..1498)
                     /locus_tag="VGB85_19150"
     CDS             complement(374..1498)
                     /locus_tag="VGB85_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEY0136214.1"
                     /translation="MSSGKSTVYWDYIKTEEILALQNGIAASDAELANDEVMFIVVHQ
                     IDELWFKLALRELVHVRDLFAQEKVREQQLANGVRGIRRTALLFEQVAHHFSLMETMT
                     TRDYLGFRDKLSPASGFQSAQLREIEILMGLKIEERLPLGHDHDYLRALKYPDGRDSP
                     ASRRVAARLNDRPTLHEAIGVWLYRTPIQGSTPEQADDEQQVNSFLERYLAAQAIELD
                     AAMGLAIDAAPGEADRARLEQRYAQEKINARAFLMAEDATPELRRRWSRIRAALLFIE
                     SYRELPLLAWPREVLDGIVHLEQAFVIFRQRHARMVERVVGRRTGTGGSAGVDYLDQT
                     ALKYRVFRDLWAVRTLLIQRSALPPLERPEVYNFVAADAE"
     gene            complement(1567..>1771)
                     /locus_tag="VGB85_19155"
     CDS             complement(1567..>1771)
                     /locus_tag="VGB85_19155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136215.1"
                     /translation="RWLAELRGEHPEPPMLVTTPAARLAFAALVQRSAPHVAVVSTAE
                     LLAVDLPLPGEPGGPATRWWSPA"
BASE COUNT          240 a          656 c          623 g          252 t
ORIGIN      
        1 cgccgggcct cgtccgcctc gacctcggtg aactcgacct cgctggcgac gttggccgcg
       61 atcgtattct tataaaaggg gtcgttcggc gcgccgatct cgagcatgcc cgtgcgcacc
      121 agcagcggct cacctgcccg ccgggacagg ccctcccaca gccgcgccgc ctcgcgcacg
      181 atcggcacgt agcccgcgcc ctcgtggtac gactcgcgga tgatccgggt gtggccgccg
      241 tggctgccct gcgtgtgcac gtggccgaag cgctcgagca cggtcacccg cgcgccgcgg
      301 cgagcgagcg cccacgcgct cgccagcccc atcgtgccgc cgccgaggac gatcaccgcc
      361 ggctgcatga cggctactcc gcgtcggccg cgacgaagtt gtagacctcg gggcgctcga
      421 gcggcggcag ggcggagcgc tggatcagca gcgtgcgcac ggcccagagg tcacggaaca
      481 cgcggtactt gagcgccgtc tggtcgaggt agtcgacgcc cgccgagccg ccggtgccgg
      541 tgcggcgacc gaccacgcgc tcgaccatcc gcgcgtggcg ctggcggaag atcacgaagg
      601 cttgctcgag gtggacgatg ccgtcgagca cctcgcgcgg ccaggcgagc agcggcagct
      661 cgcggtagct ctcgatgaac agcagcgcgg cccggatccg cgaccagcgg cggcgcagct
      721 ccggggtggc gtcctcggcc atcaggaacg cgcgggcgtt gatcttctcc tgcgcgtagc
      781 gctgctcgag gcgggcgcgg tcggcctcgc cgggggccgc gtcgatcgcc aggcccatcg
      841 ccgcgtcgag ctcgatcgcc tgggcggcga ggtagcgctc gaggaagctg ttgacctgct
      901 gctcgtcgtc ggcctgctcc ggcgtcgagc cctggatcgg cgtgcggtac agccacacgc
      961 cgatcgcctc gtgcagcgtc gggcggtcgt tgaggcgggc ggcgacgcgg cgcgaggccg
     1021 gcgagtcgcg gccgtcgggg tacttgagcg cgcgcaggta gtcgtgatcg tggccgagcg
     1081 gcaggcgctc ctcgatcttg aggcccatca ggatctcgat ctctcgcagc tgcgccgact
     1141 ggaacccgct cgccgggctc agcttgtcgc gaaagccgag gtagtcgcgc gtcgtcatcg
     1201 tctccatcag cgagaagtga tgcgcgacct gctcgaacaa cagcgccgtg cgacggatcc
     1261 cgcgcacgcc gttggcgagc tgctgctcgc gcaccttctc ctgcgcgaac aggtcgcgca
     1321 cgtggaccag ctcgcgcagc gccagcttga accacagctc gtcgatctga tgcacgacga
     1381 tgaacatcac ctcgtcgttg gcgagctccg cgtcgctcgc ggcgatgccg ttttgcagcg
     1441 cgaggatctc ctcggtcttg atgtagtccc agtagaccgt cgatttgccg ctgctcatcg
     1501 cggcggcacg ctgccacgcg ccgcccgacc ccgcaacgcc ccggggcgtg gccacccgca
     1561 caccggtcac gccggcgacc accagcgcgt cgccgggccc ccgggctcgc ccggcagcgg
     1621 caggtcgacc gccagcagct ccgccgtgct gaccaccgcc acgtgcggcg cgctgcgctg
     1681 gaccagcgcc gcgaaggcca gacgcgcagc cggcgtcgtc accagcatcg gcggctccgg
     1741 gtgctctccg cgcagctccg ccagccagcg c
//
LOCUS       DASWNJ010001135         1639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_125771, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001135 DASWNJ010000000
VERSION     DASWNJ010001135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125771"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1020)
                     /locus_tag="VGB85_19160"
     CDS             complement(34..1020)
                     /locus_tag="VGB85_19160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136216.1"
                     /translation="MSALPLLLALVVQAPDTPAATDVDPPASAPTSPAATDVDPPATV
                     PPPPAATTVEPSASVARPAVSPVHEPWRLNPPGRWFALRASAELGFVGVVKHDLQFGK
                     DGTYFDMRKEGGQDVLFPFFRFTGEFAIKQRHTLIFLIQPLKLKTTNTIDRDVRVDDV
                     EFSAGTPMAIKYDFGFYRFAYMYDLLKAPQHELGVGVALQIRNATIDFASRDGELLAS
                     NRNVGVVPLLKLRGRWTPKRGQGFWLGAEVDGAYIRGKVISGTRDYFVGALVDASIRA
                     GFEVPRAGDVFVNVRYVGGGARGTDSTPKPGKDGYAENWLHTMALSLGMYIR"
     gene            complement(1210..>1639)
                     /locus_tag="VGB85_19165"
     CDS             complement(1210..>1639)
                     /locus_tag="VGB85_19165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136217.1"
                     /translation="FVLTYKLRSAEELERIFERGAPLVVYAGGEPVIMPDSEVLVSGS
                     APRGSPTRWYCDEHIPAGAWLPESWFDLVVERGGPRGRYYGRCHGPFAQMLLVDEGTT
                     LLVRTRSAWGPDEPGSVTHVVVHLGPRYSFELMTVTLARA"
BASE COUNT          255 a          569 c          586 g          229 t
ORIGIN      
        1 cccgcgggtc cacgccgccc tcgaccacag gcatcagcgg atatacatcc cgaggctgag
       61 ggccatcgta tgcagccagt tctcggcgta gccgtccttg ccgggcttcg gggtgctgtc
      121 cgtgccgcgg gcgccgccgc cgacgtagcg cacgttgacg aacacgtcgc cggcgcgcgg
      181 gacctcgaag ccggcgcgga tcgacgcgtc gacgagcgcg ccgacgaagt agtcgcgcgt
      241 gccgctgatg accttgccgc ggatgtacgc cccgtcgacc tcggcgccga gccagaagcc
      301 ctggccgcgc ttcggcgtcc agcggccgcg gagcttgagc agcgggacga cgccgacgtt
      361 gcggttgctc gccagcagct caccgtcacg ggacgcgaag tcgatcgtcg cgttgcggat
      421 ctggagggcc acgccgacgc cgagctcgtg ctgcggcgct tttaaaagat cgtacatgta
      481 cgcgaagcgg taaaagccga agtcatactt gatcgccatc ggcgtgccgg cggaaaactc
      541 gacgtcgtcg acgcgcacgt cgcggtcgat cgtgttcgtc gtcttgagct tgagcggctg
      601 gatcaggaag atcagggtgt ggcgctgctt gatcgcaaac tcgccggtga agcgaaagaa
      661 cgggaacagt acgtcctgac cgccctcctt gcgcatgtcg aagtaggtgc catccttgcc
      721 gaattgcagg tcatgcttga cgaccccgac gaagccgagc tcggccgagg cccgcagcgc
      781 gaaccaccgc ccgggcgggt tgaggcgcca cggctcgtgc acaggcgaga cggcgggccg
      841 ggcaacgctc gcggacggtt cgacggtcgt cgcggcgggc ggcggcggga cggtcgcggg
      901 cgggtcgacg tccgtcgcgg cgggcgacgt cggggcgctc gcgggcgggt cgacgtccgt
      961 cgcggcgggc gtgtcgggag cctggacgac aagggcgagc agcagcggca gcgcggacat
     1021 cgtcgcgaag gctgatcgcg gtcggccgat ctttcaaggg tcccgcggac gctcccggac
     1081 gaggtcgaca gcatcctgtc gcagcgctcc cgcgaccggt ggcgggcgtc atgcaggtcg
     1141 gtgggtgggg tgtagccgag aacgtggacc tgcagtgacc acgcgccggt caccgtcacg
     1201 ggttcgcccc tacgcgcgcg cgagcgtcac cgtcatcagc tcgaacgagt agcgcggccc
     1261 gaggtggacg accacgtgcg tgacgctccc gggctcgtcc gggccccagg ccgaccgcgt
     1321 gcgcacgagc agggtcgtgc cctcgtcgac gagcagcatc tgcgcgaacg gcccgtggca
     1381 ccgcccatag tacctgcccc gcggcccccc gcgctcgacc acgaggtcga accacgactc
     1441 gggcagccac gcgcccgccg ggatgtgctc gtcgcaatac cagcgcgtcg ggctcccgcg
     1501 aggcgccgac cccgagacga gcacctcgct gtccggcatg atgaccggct cgccccccgc
     1561 ataaaccacg agcggcgccc cgcgctcgaa gatccgctcg agctcctccg cgctgcgcag
     1621 cttgtatgtc agcacgaac
//
LOCUS       DASWNJ010001136         4761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_21258, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001136 DASWNJ010000000
VERSION     DASWNJ010001136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21258"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..718
                     /locus_tag="VGB85_19170"
     CDS             197..718
                     /locus_tag="VGB85_19170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136218.1"
                     /translation="MPRISALILGLSLAAGCSKSSPDTAAPDDGYASEEAMADEPTER
                     PMRVTGVHLDPALTAVCELDGGPSLLELDAEAEDRMVERTLAAVAECVKTGPLQGRRL
                     EVIGHSTGGDEFRKTFGKTRAEVVRAVLVEDGVPASDLVTHAASPEDDPDAAAMWPSD
                     RRVDIRVATRHAK"
     gene            complement(726..2408)
                     /locus_tag="VGB85_19175"
     CDS             complement(726..2408)
                     /locus_tag="VGB85_19175"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEY0136219.1"
                     /translation="MSVLRTIVSSSRALVTGAKDVARFREIASVFVSHGFGWFFAQLK
                     LRRELQIELTGADLTRATVGSPDTGKRLVAAFSKLGPTFVKLGQILSTRPDLLPPGIL
                     NELTQLQDKVSPLPFDQVVSQLRHALGPGFREHFSELDETPLASASIAQVHRATLKDG
                     SRVVLKIQRPGLRKKIESDLNILRALAGWVEESFEEAAAMDLGGVLRGFAKSLAQELD
                     FNCEARNMARFRRNFADNDRVRIPKVFDELSSAEVLCMEFVEGRKFSEVLASGEDTRP
                     LVEVYFKTAYKMLFVDGFFHGDLHPGNVFVQADGGLAIIDCGMVGRLSPAMKERLIDI
                     LHAVMTEDLEQLARTFYGLAIPTGKVNYAEFEADAIEIAERYLGGVPLSEIEIGTLFS
                     ELVAGATRHNVRMPSDFTMMFKAIVTTEGLAKSIAPDIDPIELARPFITEMVAERYSP
                     ERIKHLLISDFQLISKAAHAMPRLLPQILHDIQEGKLAVGIAPATLKAHHEALAGFLT
                     KTVRTSLVITFVGCGTYCLGIAQLPLFPALGIPIMSVVFYVLALIGLVRMRS"
     gene            2547..3224
                     /locus_tag="VGB85_19180"
     CDS             2547..3224
                     /locus_tag="VGB85_19180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136220.1"
                     /translation="MKRVTTWAAAAVMMALVGGCDASRDAGAEVQRQAGEMAEKTRAG
                     AADMVAVAGERGAEAARAAADWAGEVYRSGQLSETARGWIRDGAAASQVGIEAVLQRG
                     EQAVPVATEIGKTLASAIDSDTMIEPIYQEVSGGSPELALARTEADAAISGMTRVEVI
                     DGLQVGFKELSSLDLGHSVSERAYLVVWRQGDRLVGFVYRSRRDIAIQQLVAAAPRLI
                     GLARSVL"
     gene            3271..3792
                     /locus_tag="VGB85_19185"
     CDS             3271..3792
                     /locus_tag="VGB85_19185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136221.1"
                     /translation="MIGRRLLGVSMFMLAGCGEPSLAGFCANEEVASGLELRVVDAMS
                     SGPAGQPRAGAYTFAVTTELGEFMWSCEISAADRIGLGCASDHQAAAADGMRHLLVSS
                     VASEEKFWIDLKVIDSAEWRGPAEVHVEISRDGEVVADQQFTPTYTLSPASGAEGCPN
                     YYAAEGEPPKIEL"
     gene            3895..>4761
                     /locus_tag="VGB85_19190"
     CDS             3895..>4761
                     /locus_tag="VGB85_19190"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0136222.1"
                     /translation="MLSAVASAKFTDPYNLVGATVADKYLIEAVIDRGGYGLVYRAFH
                     EILRVPVALKFFTGLAQTDPERRDELLERFAQEGALLTSLSTRSANIVQARDIGTLTT
                     EDGTFMPYLVLEWLEGQSLERLLHGSNSPARGRPHDWMDAFFLLDGVARALAIAHAAG
                     VAHRDIKPGNFFVMGEDLHPGVVIKLLDFGIAKVMPADMGMTQGLSNEFTPVYGAPEQ
                     FDRIHGATGPWTDVFGFALVMLELVRGGARVFQEREFVALAQVCQDPTRRPTPRTLGI
                     AASDAVEEVFARA"
BASE COUNT          737 a         1522 c         1798 g          704 t
ORIGIN      
        1 gagatacgtc gggcccggag cgcccgactc cgcgcaggca ggaatgcgtc gtatgtatgt
       61 atgcaccagg gcgagcgccg cgcgagccgt tatggtgcat acattaaaac agctgtcctg
      121 aggccatgga cggccgtcca cggccagcgc gtggctcgcc gctggcacga gtggcatgac
      181 aatatgtatg caaaccatgc cacgtatttc cgcgctgatc ctcggtctgt cgctggcagc
      241 cggatgcagc aagagctccc ctgacaccgc cgccccggac gatggatatg catccgagga
      301 ggcgatggcc gatgagccca ccgagcgccc gatgcgggtg accggcgtgc acctcgaccc
      361 ggcgctgacg gccgtgtgcg agctggacgg cgggccgagc ctgctcgagc tcgacgccga
      421 ggccgaggac cgcatggtcg agcgcacgct cgccgcggtc gccgagtgcg tcaagaccgg
      481 gccgctacag ggccggcgcc tcgaggtgat cggccactcg accggcgggg acgagtttcg
      541 caagacgttc ggcaagacgc gggccgaggt ggtgcgcgcc gtgctggtcg aggacggcgt
      601 cccggcgagt gacctcgtga cgcacgcggc gagcccggag gacgacccgg acgcggccgc
      661 gatgtggccg agcgaccgcc gggtcgacat ccgcgtcgcc acgcgtcacg cgaagtaaca
      721 gcggctcacg aacgcatgcg cacgagcccg atcagcgcga gcacataaaa gaccaccgac
      781 atgatcggga tgccgagcgc cgggaacagc ggcagctggg cgatcccgag gcagtaggtg
      841 ccgcagccga cgaaggtgat cacgagcgag gtgcggaccg tcttcgtaag aaagccggcg
      901 agcgcctcgt ggtgggcctt gagggtcgcc ggggcgatgc cgacggcgag cttgccctcc
      961 tggatgtcgt ggaggatctg cgggagcagg cgcggcatcg cgtgcgcggc cttggagatc
     1021 agctgaaagt cggagatcag caggtgcttg atgcgctcgg ggctgtagcg ctcggcgacc
     1081 atctcggtga tgaaggggcg cgcgagctcg atcgggtcga tgtccggggc gatcgacttg
     1141 gcgaggccct cggtcgtgac gatcgccttg aacatcatcg tgaagtccga gggcatgcgc
     1201 acgttgtggc gcgtggcacc ggcgacgagc tcggagaaca gggtgccgat ctcgatctcc
     1261 gacagcggca cgccgccgag gtagcgctcg gcgatctcga tcgcgtcggc ctcgaactcg
     1321 gcgtagttga ccttgccggt cgggatcgcc aggccataaa aggtgcgcgc gagctgctcg
     1381 aggtcctcgg tcatcaccgc gtgcaggatg tcgatcaggc gctccttcat cgccgggctc
     1441 agccgaccga ccatgccgca gtcgatgatc gccagcccgc cgtcggcctg cacgaacacg
     1501 ttgccggggt gcaggtcgcc gtggaaaaac ccatcgacga acaacatctt gtaggcggtc
     1561 ttgaagtaga cctcgacgag cggccgcgtg tcctcgccgc tcgcgagcac ttcgctgaac
     1621 ttgcggccct cgacgaactc catgcacagc acctcggcgc tgctcagctc gtcgaacacc
     1681 ttggggatcc gcacgcggtc gttgtcggcg aagttgcggc gaaagcgggc catgttgcgg
     1741 gcctcgcaat tgaagtcgag ctcctgcgcg agcgacttgg cgaagccgcg caggaccccg
     1801 ccgaggtcca tcgccgcggc ctcctcgaac gactcctcga cccagccggc gagcgcgcga
     1861 agaatgttga ggtcgctctc gatcttcttg cgcaggccgg ggcgctggat cttgaggacg
     1921 acgcggctac catccttgag cgtggctcgg tggacctggg cgatgctcgc ggaggcgagc
     1981 ggggtctcgt cgagctcgga gaagtgctcg cgaaacccgg gcccgagcgc gtgacgcagc
     2041 tgcgagacga cctgatcgaa gggcagcggg ctgaccttgt cctgcagctg cgtcagctcg
     2101 ttgaggatcc ccggcggcag caggtcgggg cgcgtcgaca ggatctgccc gagcttgacg
     2161 aaggtcggcc cgagctttga aaacgcggcg accaggcgct tgccagtgtc cgggctgccg
     2221 accgtcgcgc gcgtcaggtc ggcgccggtc agctcgatct gcagctcgcg gcgcagcttg
     2281 agctgggcga agaaccaccc gaagccgtgg ctgacgaaca cgctggcgat ctcgcggaac
     2341 ctcgccacgt ccttggcgcc ggtgaccagc gcccgcgagg agctgacgat tgtgcgcagg
     2401 acggacacgg gcgagagtat acgggtcccg gcgcggatct ggcagcggcg atccgcgtcc
     2461 gcgccggcag atccggatcc tgagccgtgg cgccgggatc gccggggctc gctctgctat
     2521 cctgacgggc tcacgaggga ggcgcgatga agcgagtcac gacctgggct gctgctgcgg
     2581 tgatgatggc gctggtgggc ggctgcgatg catcgcgcga cgcgggggcc gaggtgcagc
     2641 gccaggccgg cgagatggcc gagaagacgc gcgcgggcgc ggcggacatg gtcgcggtcg
     2701 ccggggagcg cggggccgag gcggcccggg ccgcggccga ctgggccggc gaggtgtatc
     2761 gcagcgggca gctcagcgag acggcgcgcg gctggatccg ggacggcgcg gcggcctcgc
     2821 aggtcggcat cgaagcggtg cttcagcgcg gcgagcaggc ggtgccggtg gcgaccgaga
     2881 tcggcaagac gctggcgagc gcgatcgact cggacacgat gatcgagccg atttatcagg
     2941 aggtcagcgg gggctcgccg gagctggccc tggcgcgcac ggaggccgac gctgcgatct
     3001 cgggcatgac gcgggtcgag gtgatcgacg gcttgcaggt cggcttcaag gagctctcga
     3061 gcctcgacct cgggcacagc gtcagcgagc gcgcgtacct cgtggtctgg cggcagggcg
     3121 accgcctggt cggcttcgtg taccgctcgc gccgcgacat cgcgatccag cagctggtgg
     3181 ccgcggcgcc gcggttgatc gggctggcgc gctcggtgct gtgagcccgg ggcggttgaa
     3241 gccggccgcg ctcgctggta tgcgagagcg gtgatcggac ggcgactgct cggggtgtcg
     3301 atgttcatgc tggcgggctg cggcgagccg tcgctggccg gcttttgtgc caacgaggag
     3361 gtcgcgtcgg ggctcgagct gcgcgtcgtc gacgcgatga gtagcgggcc ggcgggtcag
     3421 ccgcgggccg gcgcatatac attcgcggtg acgaccgagc tcggcgaatt catgtggtca
     3481 tgcgagatct cggccgcgga tcgcatcggc ctgggttgcg ccagcgatca tcaggccgcc
     3541 gccgcggacg gtatgcggca cctgctggtc tcgtcggtcg ccagcgagga gaagttctgg
     3601 atcgacctca aggtgatcga cagcgccgag tggcgcgggc ccgcggaggt ccatgtcgag
     3661 atctcgcgcg acggcgaggt cgtcgccgac cagcaattca cgccgacata cacgctgtcg
     3721 ccggcgagcg gcgcggaggg ttgcccgaac tactacgcgg cggagggcga gccgccgaag
     3781 atcgagctgt gagcgcgagc gtgcacgggc ttttgcgcgc gagctcgtca ggagcgcgca
     3841 caggcgcgca gggccgcggg ccgtccacgg ggccgccggg gcgaggactg tggcatgctg
     3901 tcggcggtgg cgagcgccaa gttcaccgat ccatacaacc ttgtcggggc gacggtcgcc
     3961 gacaagtact tgatcgaggc ggtgatcgat cgcggcggtt atggcctggt gtaccgggcc
     4021 ttccacgaga tcctgcgcgt gccggtcgcg ctcaagttct ttacggggct ggcccagacg
     4081 gaccccgagc ggcgcgacga gctgctcgag cgcttcgccc aggagggcgc gctgctgacc
     4141 tcgctgtcga cgcgctcggc gaacatcgtg caggcgcggg acatcggcac gctgacgacc
     4201 gaggacggca ccttcatgcc ctacctcgtg ctcgagtggc tcgaggggca gtcgctcgag
     4261 cgcctgctgc acggcagcaa cagcccggcg cgcggccggc cgcacgactg gatggacgcg
     4321 ttcttcctgc tcgacggggt cgcccgcgcc ctggcgatcg cccacgccgc aggggtcgcg
     4381 catcgtgaca tcaagccggg caacttcttc gtgatgggcg aggacctgca ccccggcgtc
     4441 gtcatcaagc tgctcgactt cggcatcgcc aaggtgatgc ccgccgacat gggcatgacc
     4501 cagggcctca gcaacgagtt cacgccggtg tacggcgcgc ccgagcagtt cgaccgcatc
     4561 cacggggcga cggggccgtg gaccgacgtc ttcggcttcg cgctggtgat gctcgagctg
     4621 gtgcgcggcg gggcccgggt gttccaggag cgtgagttcg tcgcgctcgc gcaggtgtgc
     4681 caggacccga cgcgccggcc gacgccgcgg acgctcggca tcgcggcgag cgacgcggtc
     4741 gaggaggtgt tcgcgcgggc g
//
LOCUS       DASWNJ010001137         2750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_56970, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001137 DASWNJ010000000
VERSION     DASWNJ010001137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56970"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VGB85_19195"
     CDS             complement(<1..356)
                     /locus_tag="VGB85_19195"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEY0136223.1"
                     /translation="MKRAAALSPEEPARVIHSGTSRSGVFIAYSHRDRDLLGELQVHL
                     KPLQKAWMVDLWDDFRVDAGPRGEEELRRAVAATRVAVLLVSPGLLSSPFVTEHHLPE
                     MLAAQQHPRAAVMCLYL"
     gene            458..964
                     /locus_tag="VGB85_19200"
     CDS             458..964
                     /locus_tag="VGB85_19200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136224.1"
                     /translation="MLFPLCLLLACSGNETGGSPQSAAAPATDKKVIQTTSKSPGEAP
                     TPTLHAVQASHTGPVPGPTDPPWFKPDLFPGAKVTSSGRTQKDEQGLFASQMLLALPA
                     GTTREACVDALMAAVSGSIPTLDQQPGQDRVTLTGSNDDYHVTMLCGESKGTMSAYVS
                     YRWLRPPT"
     gene            complement(1086..2003)
                     /locus_tag="VGB85_19205"
     CDS             complement(1086..2003)
                     /locus_tag="VGB85_19205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEY0136225.1"
                     /translation="MVLEVLKSMFGRRIRLPRDQVSKVPRAAKKAAEPQVAAMQAAID
                     TIAALPDLADVATTKKLPKGFFAAVQALTAAYDAYIAVVVKHTPALQSAARAGTPEGS
                     NGCYEQPVGVTGVEGLAIYRNARLWKDFAPVAQELARLGEQQFKDIQSLHTGKDPEKI
                     GMTSLAVQQGRVDFARRKEHCPLLDRTTLRCKAWEQRPFVCRMHHILDPASADPQAPD
                     WPKQAKVFNVRVPLRQQVALMQIEKRMLLTPAPFLNANILQWLQLAEGHQISEVGEAP
                     LRFAPDGQPPAKANRNRPNAKKFKDKKRK"
     gene            2121..>2750
                     /locus_tag="VGB85_19210"
     CDS             2121..>2750
                     /locus_tag="VGB85_19210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136226.1"
                     /translation="MTSPRRSSRLSTALALLGVLGLSAATTTLSACKKAADSAAPDSG
                     SGGGQTAGGGHVLRYKSAPTRLKENLNVSFSLTGGGQAASMKADLTGLLDISPSGAER
                     LRVGFSVLEVRALELTGGMKPEAKDGKPAPDAKAKLLEAKGARIIDLLGDTDKDATKG
                     LPENQKKPDAKDDEIDMNSFGSFLGLPPEMPKDGLVEGTPLKISKEEKEN"
BASE COUNT          412 a         1027 c          893 g          418 t
ORIGIN      
        1 aggtacaggc acatcaccgc ggcacgcggg tgctgctgcg cggcgagcat ctccggcagg
       61 tggtgctcgg tcacgaaggg cgagctgagc aggcccggcg acaccagcag caccgcgacc
      121 cgcgtcgccg cgaccgcgcg ccgcaattct tcctcgccgc gcgggccggc gtcgacgcga
      181 aagtcgtccc acaggtcgac catccacgcc ttttgcagcg gcttgaggtg gacttgcagc
      241 tcaccgagca ggtcgcggtc gcggtggctg taggcgatga acaccccgct gcgcgacgtc
      301 cccgagtgga tgacgcgcgc gggctcttcg gggctcagcg ccgcggctct cttcatgggg
      361 gtgctgggcg tcgccgatgt cccgcgcatg tccttacctt gtcgcaagtc tcgcgcgcat
      421 acaacctgat agactcaccg ccgccatgct tcggaccatg ttgtttcccc tgtgcctcct
      481 cctcgcgtgc agcggcaacg agaccggcgg ctcgccgcaa tcggccgccg cgccggcaac
      541 agataaaaag gtaatccaga ccacatcaaa gtcaccgggt gaagcgccga ccccgacgct
      601 gcacgcggtg caggcgtcgc acaccggtcc cgtccccggc ccgaccgatc caccatggtt
      661 caagccggac ctgttccccg gcgccaaggt caccagcagc ggccgcacgc agaaggacga
      721 gcagggtctg ttcgcctcgc agatgctgct cgcgctgccc gccggcacca cccgcgaggc
      781 ctgcgtcgac gccctcatgg ccgccgtctc cggctccatt cccacgctcg accagcagcc
      841 cggccaggac cgcgtcaccc tcaccgggag caacgacgac tatcacgtca cgatgttgtg
      901 cggcgaatcc aagggcacga tgagtgcata cgtcagctat cgctggctgc gcccgccgac
      961 ctgagccctg cggtccgccc gcgccgccgg ccgcgacgtg agcgacgagc gaatcgccgt
     1021 accggccggc gcggggtgct ggcaatcgcc gcgccggcct gcgcagggtc cgcgacggcg
     1081 cgcgcctact tgcgcttttt gtccttgaac ttcttggcgt tcggccgatt gcggttggcc
     1141 ttggccggcg gctggccgtc gggcgcgaag cgcagcggcg cctcgccgac ctcgctgatc
     1201 tggtggccct cggcgagctg caaccattgc aggatgttgg cattgaggaa cggcgccggc
     1261 gtcagcagca tgcgcttttc gatctgcatc agcgcgacct gctggcgcag cggcacgcga
     1321 acgttgaaca ccttggcctg cttcggccag tcgggcgcct gcgggtcggc gctggccggg
     1381 tcgaggatgt gatgcatgcg acacacgaac ggccgctgct cccacgcctt gcagcgcagg
     1441 gtggtcctgt cgagcagcgg gcagtgctcc ttgcggcggg cgaagtcgac gcgtccctgc
     1501 tggaccgcga ggctggtcat cccgatcttc tccgggtcct tgccggtgtg cagcgactgg
     1561 atgtccttga attgttgctc gccgaggcgc gccagctcct gggcgaccgg cgcgaagtcc
     1621 ttccacaggc gggcgttgcg gtagatcgcc aggccctcga ccccggtgac cccgaccggc
     1681 tgctcataac agccgttgct gccctcgggc gtgcccgccc gcgccgcgct ctgcagcgcc
     1741 ggcgtgtgct tgacgacgac ggcgatgtac gcgtcatacg cggccgtcaa cgcctgcacg
     1801 gccgcgaaga agcccttcgg cagcttcttg gtggtggcga cgtcggccag atccggcagc
     1861 gccgcgatcg tgtcgatggc cgcctgcatc gcggccactt gcggctcggc ggccttcttc
     1921 gccgcccgcg gcaccttgct cacctgatcc cgcggcagcc ggatccggcg gccgaacatc
     1981 gacttcaaga cttccaggac catgtgcccg cctgtctagc agagccggcc cccagtttca
     2041 gcccctcctg ggacgatccc ccgagcttcc acactcccct cgtcttgcac cgcccggccc
     2101 tcgcggttag cgtgccgccc atgacctccc cccgccgttc ctcccgcctc tcgaccgctc
     2161 tggccctgct cggcgtcctc ggcctgtccg ccgcgaccac cacgctctcc gcgtgcaaga
     2221 aggccgccga ctccgccgcc ccggacagtg gcagcggcgg cggccagacc gcgggcggcg
     2281 gacacgtgct gcgctacaag tcggccccca cccgcctcaa ggagaacctc aacgtctcgt
     2341 ttagcctcac cggcggcggt caggcggcgt cgatgaaggc cgacctcacc ggcctgctcg
     2401 acatcagccc gtccggcgcc gagcgcctgc gcgtcggctt cagcgtgctc gaggtccgcg
     2461 cgctcgagct gaccggcggc atgaagcccg aggccaagga cggcaagccc gcgccggacg
     2521 ccaaggccaa gctgctcgag gccaagggcg cgcgcatcat cgacctgctc ggcgacaccg
     2581 acaaggacgc gaccaagggg ctgccggaga accagaagaa gcccgacgcc aaggacgacg
     2641 agatcgacat gaactcgttc ggcagcttcc tcggcctccc cccggagatg cccaaggacg
     2701 gcctcgtcga gggcaccccg ctcaagatct cgaaggagga gaaggagaac
//
LOCUS       DASWNJ010001138         2194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_125867, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001138 DASWNJ010000000
VERSION     DASWNJ010001138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125867"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VGB85_19215"
     CDS             <1..267
                     /locus_tag="VGB85_19215"
                     /inference="COORDINATES: protein motif:HMM:NF022150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HEY0136227.1"
                     /translation="GGGNQGGGRGFAGMDEEKQREIASKGGQAAHEKGTAHEFDSEEA
                     REAGRKGGEAVSRDREHMAEIGSKGGQASGGNRGGNQGGGGNER"
     gene            complement(417..656)
                     /locus_tag="VGB85_19220"
     CDS             complement(417..656)
                     /locus_tag="VGB85_19220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136228.1"
                     /translation="MRTALIFAVTLTALTGCAGKLDKVTRTQAAADFKCAEDQLNVAR
                     DEVYGKYHVSGCDQKGVYSAQCLMGKCSAERLKGE"
     gene            978..>2194
                     /gene="dnaB"
                     /locus_tag="VGB85_19225"
     CDS             978..>2194
                     /gene="dnaB"
                     /locus_tag="VGB85_19225"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEY0136229.1"
                     /translation="MARTLPHNNEAEAAILGGILLRGQQALNDVMDVVREDDFYNTAH
                     QAIFKAMKVLDERREPIDVVTLEAQLRAVGELKVAGGLEALNKLGDRYATSQNIVHHA
                     ELVRRASMVRNLVITAREVAEEGMEELENSNEFIDQAEKRIMAVNERGRRTAYQSSSE
                     LLKKIFVEITERAKRQNPITGVPTHFTDLDKMTGGMQPGDLVIIAARPSMGKTAFVLN
                     LAQNACVPQARHINLPVEEQPILHPILFFSLEMGASQLVERVLCSEAKVDYSKLRSGA
                     FIDDDFRRMIGAADRISQAKFFIDDQAAPSILEMRARARRFRDDRSIFTGHKEQFGLI
                     IVDYLQLARGGKSRYDSREQEISEISRGLKAMAKELHMPVLALSQLNRAVDSRADHRP
                     QLSDLRESGAIEQ"
BASE COUNT          396 a          731 c          756 g          311 t
ORIGIN      
        1 ggcggcggca accagggcgg tggccgtggc ttcgccggga tggacgagga gaagcagcgc
       61 gagatcgcca gcaagggtgg tcaggccgcg cacgagaagg gcaccgccca cgagttcgac
      121 tccgaggagg cccgtgaggc cggccgcaag ggcggcgagg ccgtcagccg cgaccgcgag
      181 cacatggccg agatcggctc caagggcggc caggcctccg gtggcaaccg tggcggcaat
      241 cagggcggcg gcggcaacga gcgctgagcc cggagccttc agcgccgcat cgcagcgctg
      301 acgtgacgcg gtgggtcggg gtcacccggt ccaccgcgtc ttcgcgatcg tcagcgcagc
      361 actcgcggcg cccgcgtgac gcggtggggt cgggggccgc cgcgtcttaa cgatcctcac
      421 tcgcccttga gccgctccgc gctgcacttg cccatcagac actgcgccga gtacacgccc
      481 ttctgatcac aaccactgac gtgatacttg ccgtagacct cgtcgcgcgc gacgttgagc
      541 tggtcctccg cgcacttgaa gtcggccgcg gcctgcgtgc gcgtgacctt gtcgagcttg
      601 ccggcgcagc cggtcagtgc ggtgagggtg acggcgaaga tcagggcggt gcgcatgccc
      661 ggactgtatc accccccgga cggatgttcg gccagactgc tcgcgcgtgc tcgtcgagcg
      721 gccagctctc gcgcaagacc agcagacacg ccagcgcacc ggcgcgcgcc gctccgcacg
      781 cgagacgatg catgcagggc atgcgaccgc ggcgacgatg cccgcgcagc acgcgaccgg
      841 cgagcacacc gcccggacga gcgaagcgaa gtcctcgagc tgcaacgacg aatccgcggg
      901 gcatgcccgt ctccccggag agtgcgtctc cggcaggaca tccctcccga gacccgccat
      961 gctataccgc gaccccgatg gcgcgaactc tgccgcacaa caacgaggcc gaggcggcga
     1021 tcctcggcgg catcttgctg cgaggacagc aggctctcaa cgacgtgatg gatgtcgtcc
     1081 gcgaggacga tttttacaac acggcgcacc aggcgatctt caaggcgatg aaggtgctcg
     1141 acgagcggcg cgagccgatc gacgtcgtca cgctcgaggc gcagctacgg gcggtgggcg
     1201 agctcaaggt ggcgggaggt ctcgaggcgc tcaacaagct cggcgaccgc tacgcgacca
     1261 gccagaacat cgtgcatcac gccgagctgg tgcgacgcgc ctcgatggtg cgaaacctgg
     1321 tgatcaccgc gcgcgaggtc gccgaggagg gcatggagga gctcgagaac tcgaacgagt
     1381 tcatcgacca ggccgagaag cggatcatgg cggtcaacga gcgcggccgg cggaccgcgt
     1441 atcagtcgtc gagcgagctg ctcaaaaaga tcttcgtcga gatcaccgag cgggcgaagc
     1501 ggcaaaaccc gatcacgggc gtaccgacgc acttcaccga cctcgacaag atgaccggcg
     1561 gcatgcagcc gggcgacctc gtgatcatcg cggcgcgccc gtcgatgggc aagacggcgt
     1621 tcgtgctcaa cctggcgcag aacgcctgtg tgccgcaggc gcggcacatc aacttgccgg
     1681 tcgaggagca gccgatcttg cacccgatct tgttctttag cctcgagatg ggcgcgagtc
     1741 agctcgtcga gcgcgtgctc tgctcggagg ccaaggtcga ctacagcaag ctgcgctcgg
     1801 gcgccttcat cgacgacgac tttcgccgga tgatcggcgc ggcggatcgc atctcgcagg
     1861 cgaagttctt catcgacgat caggccgcgc cgtcgatcct cgagatgcgc gcacgcgcgc
     1921 gcaggttccg ggacgaccgc tccattttca cgggccataa agagcaattc ggcctcatca
     1981 tcgtcgatta ccttcagctg gcccgcggcg gcaagagtcg ttacgactcg cgcgagcagg
     2041 agatcagcga gatctctcgt ggtctcaagg cgatggcgaa ggagttgcac atgccggtcc
     2101 tggccctgtc gcagctcaac cgggcggtcg acagccgggc cgaccaccgg ccgcagctgt
     2161 ccgacctgcg tgagtcgggc gctatcgagc agga
//
LOCUS       DASWNJ010001139         3480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_56996, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001139 DASWNJ010000000
VERSION     DASWNJ010001139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56996"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..857
                     /locus_tag="VGB85_19230"
     CDS             114..857
                     /locus_tag="VGB85_19230"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0136230.1"
                     /translation="MRRVIVFGASSAIAAEVITLWAARGDRLHLVARDEHKLAAVVER
                     CAGAASVTTAVADLCDLERAPALVTEAFAALGGVDLVLVAHGELGDQERSEHDFTEAE
                     RILRTNFLSVVALLIPIADVLEQQRSGALAVMTSVAGERGRPRNYTYGAAKGALGLYL
                     QGLRSRLYPAGVQVTTLKLGPVDTPMTATHEKNALFATPARVAHDIDRAIDRGAREVF
                     VPWYWAFIMAIVRNAPEPLFQRLRFLSGR"
     gene            complement(885..2495)
                     /locus_tag="VGB85_19235"
     CDS             complement(885..2495)
                     /locus_tag="VGB85_19235"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0136231.1"
                     /translation="MARTPLLRSLVNMARKQREQPVAVERPGSGAAWTRRRFGRVIAG
                     TAAGTGALLAGCTSEAGASQPRIVIVGAGIAGLSAALELADAGIEATVYEAAGRVGGR
                     MFSNTTYFDQGQVFEWCGELIDSGHLTILALAQRFGLTLDDLLGSEPEGSEETYYFQG
                     NYYSRADAERDFLALRPALEQDLGLAGYPTTWDTSTEHGRMLDDMSVYDWIALRVPGG
                     HASPLGMLLNMAYYIELNADTREQSALNIVYLLGFQPDWADLALYGFSDEQFHIRGGN
                     QQIPVAIAESLGSDRVRLGRRMESIVRRPQGDYELLFRRASGDETVIADIVLLTLPFA
                     VLRDLDYSEAGFDDRKHRAIQELGRGRQSKQHLQFDRRVWNEKGPRPEPGTGGSYSDT
                     GYQATWETSRGQPGAAGILVGYCGGACADAMTTEVAFATATVASVRTDATDFLARAEP
                     VFPGLTAAWNGRSTQGIPHLDPNFGCSYSYWRIGQYQSIAGYEGVRQGNVFFAGEHTS
                     IDFQGFMEGGAAEGVRAAQEILAAIAGE"
     gene            2633..>3480
                     /locus_tag="VGB85_19240"
     CDS             2633..>3480
                     /locus_tag="VGB85_19240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ergosterol biosynthesis protein"
                     /protein_id="HEY0136232.1"
                     /translation="MPACFALLALYTLVFVLHLIVPGRWVTGYVHHEHTGVRLRYRLN
                     GLRVMAIMVALYFLASATGMIAWDYLYLHRWENLATACVVGLAFTAAIVLPAPPRGSL
                     LADLYLGRRENPQLLGGHVDAKMFLYLIGAVMLELNLLSFAAHHVMLYPTDPSPGVFL
                     HVGLFSFFLCEYLYFEEVHLYTYDFLAERLGFKLGWGCLTFYGFFYAVGLWSLADAPN
                     PHTPTALLVACALLFFTGWALARGANMQKYFFKRDPARAAFGLIVPEAIEQDGRRLLV
                     NGFWGLS"
BASE COUNT          486 a         1307 c         1104 g          583 t
ORIGIN      
        1 ccaggactgg cgcacgatgg ccggcctgcg cttctcggcg gccgcgcaca tcgaggcccg
       61 cgacaaggcg aaggccgccc cgtgacggcg cggtcgatgt agaattgccc ggcatgcgtc
      121 gcgtcatcgt ctttggtgcc agctcggcga tcgccgccga ggtgatcacg ctctgggccg
      181 cccgcggcga tcgcctgcac ctcgtcgcgc gcgatgagca caagctcgcc gccgtcgtcg
      241 agcgttgcgc cggtgcagcg agcgtcacga cggcggtcgc cgacctctgc gatctcgagc
      301 gcgcgcctgc gctggtcacc gaggccttcg cggcgctcgg cggcgtcgac ctcgtgctcg
      361 tcgcccacgg cgagctcggg gaccaggagc gcagcgagca cgacttcacc gaggccgagc
      421 gcatcctgcg gaccaacttc ctctcggtcg tcgcgctgct gatcccgatc gccgacgtac
      481 tcgagcagca gcgatccggc gcgctcgcgg tgatgacctc ggtcgccggc gagcgcggcc
      541 ggccgcgcaa ctacacctac ggggcggcca agggcgcgct cggcctgtac ctgcaagggc
      601 tgcgcagccg cctctatccc gcgggcgtgc aggtcaccac gctcaagctc ggcccggtcg
      661 acacgccgat gaccgccacc cacgaaaaga acgcactgtt cgccacgccg gcccgggtcg
      721 cgcacgacat cgaccgcgcg atcgaccgcg gcgcccgcga ggtgttcgtg ccgtggtact
      781 gggccttcat catggcgatc gtgcgcaacg cccccgagcc gctgttccag cgcctgcgct
      841 ttttgtccgg ccggtgaatg ttcgcacctg gcccgaaggc caggtcattc gcctgcgatc
      901 gccgcgagga tctcctgcgc agcacgcacc ccctcggccg cgccgccttc cataaaaccc
      961 tggaagtcga tcgacgtgtg ctccccggcg aagaacacgt tgccctggcg cacgccctcg
     1021 tagccggcga tgctctgata ctggccgatc cgccagtacg aatacgagca gccgaagttc
     1081 gggtcgaggt gcgggatccc ctgcgtcgag cggccgttcc acgccgccgt caaacccggg
     1141 aacaccggct ccgcgcgcgc gaggaagtcc gtcgcgtcgg tgcgcacgct ggcgaccgtc
     1201 gcggtagcga acgcgacctc ggtcgtcatc gcgtcggcgc aggcgccccc gcaatacccg
     1261 acgaggatcc cggccgcgcc cggctgcccg cgcgaggtct cccaggtcgc ctgatagccg
     1321 gtgtccgagt agctccctcc cgtgccgggc tcggggcgcg ggcccttttc gttccacacg
     1381 cggcggtcga actgcaggtg ctgcttgctc tgccgcccgc gcccgagttc ctggatcgcg
     1441 cggtgcttgc ggtcgtcgaa gccggcctcg ctgtagtcga ggtcgcgcag caccgcgaac
     1501 ggcagcgtca gcagcacgat gtcggcgatc accgtctcgt cgccgctggc gcggcgaaac
     1561 agcagctcgt agtccccctg cggccggcgc acgatcgact ccatgcgccg tccgagtcgc
     1621 acgcggtcgg agccgagcga ctccgcgatc gccaccggga tctgttgatt acccccacga
     1681 atatgaaatt gctcgtcgga gaagccatat aacgcgaggt ccgcccagtc cggctgaaag
     1741 cccagcagat agacgatgtt gagcgcgctc tgctcgcggg tgtcggcgtt gagctcgatg
     1801 taatacgcca tgttgagcag catccccagc ggcgacgcgt gcccgcccgg gacccgcagc
     1861 gcgatccagt cgtacacgct catgtcgtcg agcatgcgcc cgtgctcggt gctggtgtcc
     1921 caggtcgtcg gatagccggc gagccccagg tcctgctcga gcgccggccg cagcgcgagg
     1981 aagtcgcgct ccgcgtccgc ccgcgaatag taattgccct gaaaatagta cgtctcctcg
     2041 ctgccctccg gctcgctgcc gagcaggtcg tcgagcgtga gcccgaagcg ctgcgcgagc
     2101 gcgaggatcg tcaggtgccc gctgtcgatc agctcgccgc accactcgaa cacctggccc
     2161 tgatcgaaat atgtggtgtt ggagaacatc cgcccgccga cccgacccgc cgcctcgtag
     2221 acggtcgctt cgatcccggc gtcggccagc tcgagcgccg cgctcagccc cgcgatcccc
     2281 gccccgacga tcacgatccg cggctgactc gcccccgcct cgctcgtgca cccggccagc
     2341 agcgcccccg tgcccgccgc cgtgcccgcg atgacacggc caaagcgccg acgcgtccac
     2401 gcggcgccgc tgccaggccg ctccacggcg accggctgct cgcgctgctt cctcgccata
     2461 ttgaccagtg accgcaacag gggtgttcgc gccatctcgt gtactccgcc gctgtaaaag
     2521 cggcaccggc ttacagagcc gccgatgcgg tgtcaaggcc gccccacgcg gacgatgcga
     2581 acgtcgggcg ccgggatgga caccgcgggc cccccctcgc taccctctcg cggtgcccgc
     2641 ctgcttcgcg ctgctcgccc tgtacacgct cgtcttcgtg ctgcacctga tcgtgccggg
     2701 ccgctgggtg accggctacg tgcaccacga gcacacgggc gtgcgcctgc gctaccgcct
     2761 caacggcctg cgcgtgatgg cgatcatggt cgcgctctac ttcctcgcca gcgcgaccgg
     2821 catgatcgcc tgggattatt tatatctgca ccgctgggag aacctcgcca ccgcgtgcgt
     2881 cgtcggcctc gccttcaccg ccgcgatcgt cttgcccgcc ccgccgcggg gctccctgct
     2941 cgccgacctg tacctcgggc gccgcgagaa cccgcagctg ctcggcggtc atgtcgacgc
     3001 caagatgttt ttgtatctga tcggcgccgt gatgctcgag ctcaatctcc tgagcttcgc
     3061 ggcgcaccac gtcatgctgt acccgacgga cccgtctccg ggcgtatttt tgcatgtcgg
     3121 cctgttctcg ttcttcctct gcgagtacct gtatttcgag gaggtacacc tctacaccta
     3181 cgactttctc gccgagcgcc tcggcttcaa gctcggctgg ggctgcctga cgttttacgg
     3241 gttcttttac gcggtcggcc tgtggtcgct cgccgacgcg cccaaccctc acacccccac
     3301 cgcgctgctg gtcgcctgcg ccctgctgtt cttcaccggc tgggcgctcg cccgcggcgc
     3361 caacatgcaa aagtactttt ttaagcggga cccagcccgc gcggcgttcg gtctgatcgt
     3421 gcccgaggcg atcgagcagg acggccgccg cctgctcgtc aacggctttt gggggctctc
//
LOCUS       DASWNJ010001140         6131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_586323, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001140 DASWNJ010000000
VERSION     DASWNJ010001140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6131
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586323"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VGB85_19245"
     CDS             <1..425
                     /locus_tag="VGB85_19245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136233.1"
                     /translation="LAEQGMGGSAVTVSNIVFVGFGVAVPWVAAHTGGLLVSAMDGFL
                     ARLGLLRWLLALPRVLLLLALWMAIVAVSLPIGFTILAVGLVCLIWFSLDDVVATILG
                     HDEDDSTALPVELTQIVREQSPPRDPPLALPRTAGGMS"
     gene            422..1420
                     /locus_tag="VGB85_19250"
     CDS             422..1420
                     /locus_tag="VGB85_19250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136234.1"
                     /translation="MSHAHALSALAGLVVVAAALGGMMSWQSSTDTEARAAAEARGEW
                     RPPCAQRLGSAYAAIDPVPKVNDSPPRTLLFGVDRSPSNLALADEQMDAVVAHAATLP
                     AETGVGVLLISDRSERSSTPDMPFEAASPRRSFTPAPLPCAPDCTADSLFKKKCLEQI
                     DEAQARRITTLDLGESQARQTAQEQRAERIHRWREQVRSWTPKPGTSLLGFWAKVADL
                     PVIRRTPERTTVVVFSDLEEARTPDRKKVEQFYKQYQARGACPEAAWLPQGIGGLDIV
                     LVQTVSDGIDATAWGERWEAVLTCAGAHVRRHRYSPAISVAEVLGPDEPARVATVD"
     gene            complement(1437..2339)
                     /locus_tag="VGB85_19255"
     CDS             complement(1437..2339)
                     /locus_tag="VGB85_19255"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0136235.1"
                     /translation="MRGPSLIAGVLSMMLSLAACGGSDAAQRAPLWPGGAPGAKGDGK
                     GDRPELYYYLPAPEKRTGLAVIVAPGGSYAHTGGLRVEAFPTARWLAKQGITAIVLRY
                     RVSNDDYHHRDFLADGKQAVRIVRAQASKLGVDPQKIGFIGFSAGGHLAAFVGTACPP
                     DGDPDDPDPLMRVSCRPDFIVMVYPVVTLDDQWAHQRSKKSLLGPEDPPPLGLVDRLS
                     LEKQVGPTTPPAFLVHSRRDSKVLFHNSELFHDAMLASGRPSELHLYDDGKHGVGLAQ
                     KPGMPGMRQWPAQMLAWLHGLGML"
     gene            2414..3016
                     /locus_tag="VGB85_19260"
     CDS             2414..3016
                     /locus_tag="VGB85_19260"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0136236.1"
                     /translation="MRLYGTLTSPFVRRVRVVALELHVELEFVDTTTPEGQAGLATLS
                     PLRKVPVLEVDGAGVLDSRTISALLLERHGPGDLRSPRASTRWIEDSLIHAVDGALES
                     AIRLFYFIKRDGVDVAPIPYMRTERERVDRTLAWLEGQIHGPWCTAAEGFGLAELALV
                     TTLEWMHFRGMAELATYPNLQAFAAHHAGRPSLVRTRPPV"
     gene            complement(3036..4667)
                     /locus_tag="VGB85_19265"
     CDS             complement(3036..4667)
                     /locus_tag="VGB85_19265"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0136237.1"
                     /translation="MRPATSSHPLRLLSGLALCAAMLSPGLADGRSPAPREREEEREK
                     PKKMEMVHPMRMAAPPAAYGFSADLEMGATPGGAQDIAFFRDRAAKGEVPHPNTFTPE
                     GLFSEHDLPLATRTKCMQTLCATGEAIGAGLLVQPEVHYLAQLGFSSGLDPKTFKRAP
                     LHLVAVIDKSGSMSGQPLELVKASLQKVLTQLGPDDELSIVLYGDRSHVYMQPTPVKD
                     RAAIAREIRKIESAGSTNMEEGLAVGFSLAQSHRFAGSTRVMLFTDERPNVGSTDKHS
                     FMGMARDASKSGVGMTTVGVGVQFGAELATAISSVRGGNLFFFPDSKRMEKVFEEEFD
                     TMVTELAHDLALEVRPAKGLKIAGVYGIPGQALKWIDGGAISLSVETIFLSRKKGAIY
                     IAMASDTGPGLPETKVAPGSGVASVKLDYQERSGKAVRAQVELPTVETSAASLGLVRG
                     HLLVNQATSLARAASLHHEKNDQEGAYQAVHALASLFRRSTDSTLANERKLVLDLERT
                     LAKLSGHQGEPNSGSGHRPEGGPVDRVSGLPPRVR"
     gene            5029..5997
                     /locus_tag="VGB85_19270"
     CDS             5029..5997
                     /locus_tag="VGB85_19270"
                     /inference="COORDINATES: protein motif:HMM:NF017393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HEY0136238.1"
                     /translation="MKLSPCFLFVGAMMFVACDASEPEQSSALPVAEVEAEEDAAQCL
                     MDATPAACEVELASAPRHRRCAMHVTEIQAATLEADLLNRLALAPNPSAGKPPPSPSP
                     SPTGAAVPVWFHVITDGSADGNISDNALTAQLSVLNETYVGTGFSFTHAGTTRTTNAA
                     WYAMDAAAETQAKSALRKGGANTLNIYLANPGSGYLGWATFPSSYASNPKADGVVILN
                     QSVPGGKAVPYDLGITAVHEVGHWIGLYHTFQGGCAKTGDYVEDTPPEKSAAFGCEIG
                     RDTCPATGLDPIHNFMDYSDDACLDQFSAGQTARMKTMWSTYRANG"
BASE COUNT          894 a         2194 c         2080 g          963 t
ORIGIN      
        1 tgctcgccga gcagggcatg ggcggctcgg ccgtcaccgt ctccaacatc gtgttcgtcg
       61 gcttcggcgt cgcggtcccg tgggtcgcgg cgcacaccgg cggcctgctg gtctcggcga
      121 tggacggctt cctcgcccgg ctcggcctcc tgcgctggct gctcgcgctg ccgcgtgtac
      181 ttctgctgct cgcgctgtgg atggcgatcg tcgccgtctc gctgccgatc ggctttacaa
      241 ttctcgcggt cggcctcgtg tgcctgatct ggttctcact cgacgatgtc gtcgcgacga
      301 tcctcggcca cgacgaggac gacagcaccg cgttaccggt cgagctgacg cagatcgttc
      361 gcgaacagtc ccccccacgc gacccgccgc tggccctgcc gcgcaccgcc ggaggtatgt
      421 cgtgagccac gcccacgccc tctccgccct cgccgggctc gtcgtcgtcg cggcagcgct
      481 cggcggcatg atgtcctggc agtcgagcac cgatacagag gcccgcgccg ccgccgaggc
      541 ccgcggcgag tggcgcccgc cctgcgcgca gcgcctcggc agcgcctatg cggcgatcga
      601 cccggtgccg aaggtcaacg acagccctcc gcgcacgctg ctgttcggcg tcgaccgcag
      661 cccctcgaac ctcgccctcg ccgacgagca gatggacgcc gtcgtcgcgc acgcagccac
      721 tctgccggcc gagaccggcg tcggcgtgct gctgatctcg gaccgcagcg agcgctcctc
      781 gactcccgac atgcccttcg aggccgcgag cccgcgccgc agcttcaccc ccgcgcccct
      841 gccctgcgct cccgactgca ccgccgactc gttgttcaaa aagaagtgcc tcgagcagat
      901 cgacgaggcc caggcgcgtc gtatcaccac gctcgacctc ggcgagtccc aagctcgcca
      961 gaccgcgcag gagcagcgcg ccgagcggat ccatcgctgg cgcgagcagg tgcgcagctg
     1021 gaccccgaag cccggcacct cgctgctcgg cttctgggcc aaggtcgccg acctgcccgt
     1081 catccgtcgc acgcccgagc gcacgaccgt ggtcgtgttc tccgacctcg aggaggcgcg
     1141 cacgccggac cgcaagaagg tcgagcaatt ttataagcag taccaggctc gcggcgcctg
     1201 cccggaggcg gcatggttgc cgcagggcat cggcgggctc gacatcgtgc tcgtgcagac
     1261 cgtcagcgac ggcatcgacg cgaccgcgtg gggtgagcgc tgggaggccg tgctcacctg
     1321 cgcgggcgcc cacgtccgcc gccaccgcta cagccctgcg atctcggtcg ccgaagtgct
     1381 cggccccgac gagcccgccc gcgtcgccac cgtcgattga ttaatttaaa taattatcaa
     1441 agcatgccga gcccgtgcag ccacgcgagc atctgcgcgg gccactggcg catccccggc
     1501 atgcctggct tttgggccag gccgacgccg tgcttgccgt cgtcataaag gtgcagctcg
     1561 gagggtcggc cgctggcgag catcgcgtcg tggaacagct cgctgttgtg aaacaggacc
     1621 ttggagtcgc ggcgcgagtg gaccaaaaac gcgggcggtg tggtcgggcc gacctgcttc
     1681 tcgagcgaca ggcggtcgac gagcccgagc ggcggcgggt cctcggggcc gagcaggctc
     1741 tttttgctgc gctggtgggc ccattgatcg tcgagggtga cgaccggata gaccatcacg
     1801 atgaagtccg ggcgacagct gacgcgcatc agcgggtcgg ggtcgtcggg gtcgccgtcc
     1861 ggtgggcacg cggtgccgac gaaggccgcg aggtggccgc cggccgagaa gccgatgaag
     1921 ccgatcttct gcgggtcgac gccgagcttg ctcgcctgcg cacgcacgat gcggaccgcc
     1981 tgcttgccgt cggccaggaa gtcgcgatga tgataatcgt cgttgctgac gcgatagcgc
     2041 agcacgatcg cggtgatgcc ctgcttggcg agccagcgcg cggtcgggaa cgcctcgacg
     2101 cgcaggccgc cggtatgtgc ataactgccg ccgggcgcga cgatcaccgc gaggccggtc
     2161 cgcttctcgg gcgccggcaa gtagtaataa agctcgggcc ggtcgccctt gccgtccccc
     2221 ttggcccccg gcgccccgcc cggccacagc ggcgcgcgct gggccgcgtc cgacccgccg
     2281 caggcggcga gcgacagcat catggagagc accccggcga tcagcgaagg tccgcgcacg
     2341 tgcccgcact gtcggacgtc tcgcggacaa ttccaagccc cccgccgcgt gcgggtcctg
     2401 atataccggc ggcatgcgcc tgtacgggac cctgacctcc cccttcgtgc gtcgcgtccg
     2461 cgtggtcgcc ctcgagctcc acgtcgagct ggaattcgtc gatacgacca cgccggaggg
     2521 ccaggccggg ctcgccacgc tgtcgccgct gcgcaaggtc cccgtgctcg aggtcgacgg
     2581 cgccggtgtg ctcgactcgc gcacgatctc ggcgctgctg ctcgagcgtc acggcccggg
     2641 tgacctgcgt tcgccgcgcg cgagcacgcg gtggatcgag gacagcctga tccacgccgt
     2701 cgatggcgcg ctcgagtccg cgatccgcct gttttatttc ataaaacgcg acggggtgga
     2761 cgtcgcgccg atcccctaca tgcggaccga gcgcgagcgg gtcgaccgga ccctcgcctg
     2821 gctcgaaggc cagatccacg gcccgtggtg caccgccgcc gagggcttcg gcctcgccga
     2881 gctcgcgctg gtgacgaccc tcgaatggat gcactttcgt gggatggccg agctggccac
     2941 atatcccaac ctccaggcat tcgcggccca ccatgcgggc cgccccagcc tggtgcgcac
     3001 gcgcccgcct gtctgagcgt ttcgcgtcaa ctctttcagc gcacccgcgg cgggagcccg
     3061 cttacgcggt cgaccgggcc gccctcgggg cgatgaccgg agccgctgtt cggctcgccc
     3121 tgatgacccg agagcttggc gagcgtgcgc tcgaggtcga ggaccagctt gcgctcattc
     3181 gccaaagtgc tgtcggtgct gcggcgaaac agcgaggcga gcgcgtgcac ggcctggtaa
     3241 gcgccctcct ggtcgttctt ctcgtggtgc aggctggcgg cgcgggccag gctggtcgcc
     3301 tggttgacga gcagatgccc acgcaccagg ccgaggctgg cggcgctggt ctcgacggtc
     3361 ggcagctcga cctgcgcgcg cacggccttg ccgctgcgct cctggtaatc gagcttcacg
     3421 ctggcgacac cggagccggg cgcgaccttc gtctcgggca gcccgggccc ggtgtccgac
     3481 gccatcgcaa tgtagatcgc gcccttttta cgactgagga agatcgtctc gaccgacagc
     3541 gagatcgcgc cgccgtcgat ccacttcaat gcctgcccgg ggatcccgta cacgccggca
     3601 atttttaacc ccttcgccgg gcggacctcg agcgcgaggt cgtgggcgag ctcggtcacc
     3661 atcgtgtcga actcctcctc gaacaccttc tccatgcgct tgctgtcggg gaaaaagaac
     3721 aggttgccgc cgcgcacgct gctgatcgcc gtcgccagct cggcgccgaa ctgcacgccg
     3781 acgccgacgg tcgtcatgcc gacgccggac ttcgaggcgt cgcgggccat gcccatgaag
     3841 ctgtgcttgt cggtgctgcc gacgttcggg cgctcatcgg tgaacagcat cacgcgcgtg
     3901 ctgccggcga accggtgcga ctgcgccagc gagaagccga ccgcgagccc ctcttccata
     3961 ttcgtcgagc cggcgctctc gatcttgcgg atctcgcggg cgatcgcggc gcggtccttg
     4021 accggggtcg gctgcatata gacgtgcgag cgatcgccgt agaggacgat cgagagctcg
     4081 tcgtccgggc cgagctgcgt gagcaccttt tgcaagctcg ccttgacgag ctcgagcggc
     4141 tggccggaca tcgagcccga cttgtcgatc accgcgacca gatgcagcgg cgcgcgcttg
     4201 aaggtcttcg ggtcgaggcc cgaggagaag ccgagctgcg cgaggtaatg gacctccggc
     4261 tggaccagca ggcccgcgcc gatcgcttcg ccggtggcac acagcgtctg catgcacttc
     4321 gtgcgggtcg ccagcggcag gtcgtgctcg ctaaacaggc cctccggcgt aaaggtgttc
     4381 ggatgcggga cctcgccctt cgcggcgcgg tcgcggaaaa atgcgatgtc ctgcgcgcca
     4441 cccggggtcg cgcccatctc gaggtccgcc gagaagccgt aggcggccgg aggcgcggcc
     4501 atccgcatcg ggtgcaccat ctccatcttc ttcggcttct cccgctcctc ctcgcgctcg
     4561 cgcggcgcgg ggctgcggcc gtcggcgagc ccgggtgaca gcatcgcagc gcacagggcg
     4621 aggccggaga ggaggcgaag aggatgagac gaggtggcgg gtcgcatgtg ccgggctccg
     4681 tacgcgcgag cgccggcccg ggtctgtgat gatgaaaaag ttcgcgggcg ccggcgaaca
     4741 ttctgcgcgc ggaggtcagc cgggcgaaca atgtgtatag tctctggcct cgactgtcgc
     4801 tatcgcggcc gcgctttgcc tgggcaatgt cagggcgcgt ccgaccactg ggggctctgg
     4861 actacgcatg gggccatcgc ggcgctcacg gggattccag ggtcggcaga cgtctccggc
     4921 gtgaattgtc cgacgccggg gggctgttcg gcggctgcct cgccgggggt gatgcgtata
     4981 tgatgcggtc cgcggttggt tgaacgccgc ggaatggaac gcgatcccat gaagctgagc
     5041 ccctgtttcc tgttcgtcgg tgcgatgatg ttcgttgcct gtgatgcgtc ggagccggag
     5101 cagagctccg cgttgcccgt cgccgaggtc gaggccgagg aggacgccgc gcagtgtctg
     5161 atggacgcga cgcccgcggc ctgcgaggtc gagctggcct ctgccccgcg ccatcgtcgg
     5221 tgtgccatgc acgtcaccga gatccaggcc gcgaccctcg aggccgacct gctcaaccgc
     5281 ctcgcgctgg cgccgaaccc gagcgcgggc aagcccccgc cctcaccctc gccctcgccc
     5341 accggcgccg cggtcccggt gtggttccac gtgatcaccg acggcagcgc cgacggcaac
     5401 atctccgaca acgccctgac ggcccagctc tcggtcctca acgagaccta cgtcggcaca
     5461 ggattcagct tcacgcacgc gggcacgacc cgcacgacca acgccgcgtg gtacgcgatg
     5521 gacgcggccg ccgagacgca ggccaagagc gcgctgcgca agggcggcgc caacacgctc
     5581 aacatctacc tcgccaaccc cggcagtggt tacctcggct gggcgacctt cccgtcgtcc
     5641 tacgcgagca acccgaaggc cgacggcgtc gtcatcctca accagagcgt gcccggcggc
     5701 aaggcggtcc cctacgacct cggcatcacc gcggtccacg aggtcggcca ctggatcggc
     5761 ctttatcaca ccttccaggg cggctgcgcc aagaccggtg attacgtgga ggacacgccg
     5821 cccgagaagt ccgccgcctt cggctgcgag atcggccgcg acacctgccc cgccaccggc
     5881 ctcgacccga tccacaactt catggactac tcggacgacg cctgcctcga ccagttcagc
     5941 gccggccaga ccgctcgcat gaagacgatg tggtcgacgt accgcgcgaa cggctgagcg
     6001 agcgcgcccg gggcgtccgg gggtgctctg cgatcgcggg ccgggaacac accggcccgc
     6061 gtgtgtgcag atttagcggc ggcgccggga cgcgtccgcc gcccctttca tgatacctgt
     6121 ccgtcatgaa g
//
LOCUS       DASWNJ010001141         1532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_160077, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001141 DASWNJ010000000
VERSION     DASWNJ010001141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160077"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VGB85_19275"
     CDS             <1..750
                     /locus_tag="VGB85_19275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136239.1"
                     /translation="PLIPHIGPPSYLLPLDILFSASKSYFGTTEYKVEGKPAAVAILF
                     IVNLNLNCGTPFPKRPGVVLAFNTHFAGMTWGDFFHGLLSGLIDWTLQFLLSKALGKF
                     GDWFTAKLAPAALSKAAVKGMINRSSVPKGMSINVFARQIAQQNMAKTERLMALFPAF
                     MRPRAQLVNPIVNAVSGTVAGFFVGGPLGMDAAVIGAPTPGGGMTNGAQDYLNNNVVD
                     EFGNPPPPPPPPPRQPPPPRRPPSIGPARCQ"
     gene            747..>1532
                     /locus_tag="VGB85_19280"
     CDS             747..>1532
                     /locus_tag="VGB85_19280"
                     /inference="COORDINATES: protein motif:HMM:NF021456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HEY0136240.1"
                     /translation="MTVVSRPGPTVAQLSDVLPTGEPMLSVVARRTYTLERGRLVPAD
                     EQSPLVGQPVMDREHPLLLAADSDLYAYKPRSDVVIHGHAYATQGSGRAELVVRVDRH
                     EHRVAVFGERRVSLTGQRLRFSEPAPIERVPLHHGSAYGGRDRGGEAVHGNPLLTDPS
                     LVRGFEGVDLDAASPFIYPRNPAGRGYLVESTPGAVEQLQLPLLEDPTDLLSPERIVA
                     GWVDQWPRMPLPYATGWIDYNWYPRVAFFGIVPMVDFFEQPPRE"
BASE COUNT          215 a          595 c          497 g          225 t
ORIGIN      
        1 ccgctgatcc cccacatcgg cccgccgtcg tacttgctgc cgctcgacat cctgtttagc
       61 gcgtcgaagt cgtacttcgg caccaccgag tacaaggtcg agggcaagcc ggcagcggtg
      121 gcgatcctgt tcatcgtcaa cctcaacctc aactgcggca cgccgttccc caagcgcccc
      181 ggggtggtgc tggccttcaa cacccacttc gccggcatga cctgggggga cttcttccac
      241 ggcctgctca gcggcctgat cgactggacc ctgcagtttc tgctcagcaa ggcgctcggc
      301 aagttcggcg actggttcac cgcgaagctg gcaccggccg cgctgtcgaa ggccgccgtc
      361 aagggcatga tcaaccgctc gtcggtgccc aagggcatgt cgatcaacgt gttcgcgcga
      421 cagatcgccc agcagaacat ggccaagacg gagcgcctga tggcgctgtt ccccgcgttc
      481 atgcgcccgc gcgcccagct ggtcaacccg atcgtcaacg cggtcagcgg caccgtcgcc
      541 ggcttcttcg tcggcggccc gctcggcatg gacgccgccg tcatcggcgc gccgaccccc
      601 ggcggcggga tgaccaacgg cgcgcaggac tacctcaaca acaacgtcgt cgacgagttc
      661 ggcaacccgc cgccgccacc accgccgcca ccgcgacagc cgccgccgcc gcgcaggccg
      721 ccgagcatcg gccccgcgag gtgccagtga ccgtcgtctc tcgcccgggc cccaccgtcg
      781 cgcagctgtc ggacgtgttg ccgaccggcg agccgatgct ctcggtcgtc gcgcgccgca
      841 cgtacacgct cgagcgcggc cgcctggtgc cggccgacga gcagtcgccc ttggtgggtc
      901 agccggtgat ggaccgcgag catccgctgc tgctcgccgc cgactccgac ctgtacgcct
      961 acaagccgcg cagcgacgtc gtgatccacg gccacgccta cgcgacgcag ggcagcggtc
     1021 gcgcggagct ggtggtccgg gtcgaccgcc acgagcatcg cgtcgccgtg ttcggcgagc
     1081 gccgcgtctc gctcaccggg cagcgcctgc gcttctccga gccggcgccg atcgagcggg
     1141 tgccactgca ccacggctcg gcctacggcg gccgcgaccg cggcggggag gccgtccacg
     1201 gcaacccgct gctcaccgac ccgtcgctgg tccgcggctt cgagggcgtc gacctcgacg
     1261 ccgcctcgcc gttcatctac ccgcgcaacc cggccggccg cggctacctg gtcgagtcga
     1321 cgcccggggc cgtcgagcag ctgcagctgc cgctgctcga ggaccccacc gacctgctga
     1381 gccccgagcg catcgtcgcc ggctgggtcg accagtggcc gcggatgccg ctgccgtacg
     1441 cgaccggatg gatcgactac aactggtacc cgcgcgtcgc cttcttcggg atcgtgccga
     1501 tggtcgactt cttcgagcag ccgccgcgcg ag
//
LOCUS       DASWNJ010001142         6284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_125911, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001142 DASWNJ010000000
VERSION     DASWNJ010001142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6284
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125911"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="VGB85_19285"
     CDS             complement(<1..1102)
                     /locus_tag="VGB85_19285"
                     /inference="COORDINATES: protein motif:HMM:NF019321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0136241.1"
                     /translation="MALSLMPGQIERVFPFHLAVDQSGRLTQLGPGLRKLLPGVFVGD
                     AFDAAFTVARPMLRSTGVAAMLARADTLFILEARARTFKLRGQVLAVGEELLFLGTPW
                     ITELGQLQQLGLHFKDFALHDATTDILMVMNTVRTSLTDARKLAEQLGERTAQLQEAT
                     RLAEEASAAKSAFVANMSHELRTPLNAILGYSEMLLEDVEVAGNAEAAADLRRIELAG
                     KHLLSLIASVLDISRIEARKIDLSIRPCSLRTVIESVHATVGPLAGPRVQLSISAVPD
                     LEMVTDEDKLRQILINLLGNACKFTTDGEIRLDVREVGPEVELVVSDTGMGMSAQQLD
                     RLFEAFYQADSSPRRRHDGAGLGLAISRAFIEA"
     gene            complement(1109..1681)
                     /locus_tag="VGB85_19290"
     CDS             complement(1109..1681)
                     /locus_tag="VGB85_19290"
                     /inference="COORDINATES: protein motif:HMM:NF019320.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme NO-binding domain-containing protein"
                     /protein_id="HEY0136242.1"
                     /translation="MYGLVNKAVEDLVCTRFGADVWNQIRTRAGIGVDSFISMDPYPD
                     DVTYKLVGAASEVLGLPPATVLEAFGEFWIRYTAKEGYGEMFSMFGSSLGEFLHSLDA
                     LHTHIGLSFSQLRPPTFDCQDLGEGEYLLSYFSSREGLAPMMIGLLKGLAATFNKNVE
                     VEHVKSRAEVGHDEFHIRELAAPAPESAAS"
     gene            complement(1766..2647)
                     /locus_tag="VGB85_19295"
     CDS             complement(1766..2647)
                     /locus_tag="VGB85_19295"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY0136243.1"
                     /translation="MTALGYVVLCLIWGSTWLGIKMGLDAGMPPLLGVCLRFAVAALV
                     LVPLAWWTGRPAFRDRVAWRLALLIGVCSFGVGYGCTYIGGMYVPSGLGSLTFGVFPF
                     WVAVLAHFILGDRLTAGKIGAIATGLAGLFTLYSGSLRELGPESLLGVAIITASVLIQ
                     GVGQVYVKRDGANVPSAFLSGMGMLIGSVLLALMAGVRGEWSEPFPWTLPVIGSIAYL
                     AVFGSIITFLVYYRLLKQLSATLMAMVALITPPIAVVLGLLFRGERLGIITMTGGALV
                     LLGVLLFQLAERRTARK"
     gene            complement(2786..4651)
                     /locus_tag="VGB85_19300"
     CDS             complement(2786..4651)
                     /locus_tag="VGB85_19300"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEY0136244.1"
                     /translation="MKFSTYSRVVGPVNPGKTVALATVLALTACPGGGETTTTTSSTT
                     DVGTTTGTTGDIEPTGGTTGDTSTTDAPTSSSTTGSTTDPVGTTDTTDTASTTDVSTS
                     TTDASTSTTDTTGGSSSTGADAVCGDGEVEQDEACDDANAGLGDGCTAECTRECTALH
                     FEGKTIAQAANPTTLHTISVTAAGWHKADATAPYGSIFTKSGKDVGGHVTYSVATGNT
                     GTTARLQVGLEAFLDLTAPTVKPDGKWHHFALTYDAVTGNGVLYVDGVVVDSEKKGMG
                     LVAANPALPFSIGAEPLMGKLFYASKADITDVVVYGTALTAGEVKGLIDGVYPPLALA
                     FYPTPEGMGAMSEDVSGNGHTLTVPDGGWAADGPYCVDAVCGNGEVDADEACDDADHD
                     LGDGCTSACKQECTALHFEGKTLAQAADPVKLHTVSLTAAGWHKAAANAPYGVIFVKA
                     GKDVGGHSTYAIATGMTGTTARLQVGLEAFLDLTAPMAKSDGMWHHYALTYDAATGNG
                     VLYRDGVVLDSEKKGMGLVAANPALPFSIGAEPLMGQLFYASKADITDVVVYGTALGE
                     ADVMGLVEGVYPPAPLAFYPTPEGTGLMSQDMSGNGHTLTVPVAGWAADGPYCAP"
     gene            complement(5037..>6284)
                     /locus_tag="VGB85_19305"
     CDS             complement(5037..>6284)
                     /locus_tag="VGB85_19305"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEY0136245.1"
                     /translation="GHITYTIANGMPGTTARLQVGPLAFLDLTAPTVKPDGTWHHYAV
                     TYDAVTGNGVLYRDGVVIDSEIKGKGLVATNPALPFSIGSGILQGNLEAASKADITDV
                     VVYGTALTADEVKGLVEGVYPPLALAFYPTPEGMGAMSEDVSGNGHTLMVPDGGWVDD
                     APYCADRSCGNGEVDMDEACDDANYNLGDGCTSACTKECTALHFEGKTAVQAANPATL
                     SPVSVTAAGWHKAAANAPYGAIVSKAGQDVGGHITYTIANGMPGTTARLQVGPAIFID
                     LSAPTAKSDGTWHHYAVTYDAATGNGVLYRDGVVLDSEKKGMGLVAVNPALPFSIGSG
                     ILQGNLEAASKADITDVVVYGMALSEAEVMGLAEGVYPPAPLAFYPTPEGTGLMSQDM
                     SGNGHTLTVPDGGWVADGPYCAP"
BASE COUNT          958 a         2274 c         2072 g          980 t
ORIGIN      
        1 gcgcctcgat gaacgcgcgg gagatcgcca ggccgaggcc ggcgccgtcg tggcggcggc
       61 gcggcgacga gtcggcctga tagaacgcct cgaacagccg gtcgagctgc tgcgccgaca
      121 tgcccatgcc cgtgtcgctg accacgagct cgacctcggg cccgacctcg cggacatcga
      181 gccggatctc gccgtcggtc gtgaacttgc acgcgttgcc gagcaggttg atcaagatct
      241 ggcgcagctt gtcctcgtcg gtgaccatct cgaggtccgg gaccgccgag atcgacagct
      301 gcacgcgggg ccccgcgagc gggccgaccg tcgcgtgcac gctctcgatc accgtgcgca
      361 gcgagcacgg ccggatcgac aggtcgatct tgcgcgcctc gatgcgcgag atgtcgagca
      421 cgctggcgat cagcgacagc aggtgcttgc ccgccagctc gatccgccgc aggtccgccg
      481 ccgcctccgc gttcccggcg acctcgacgt cctcgagcaa catctcgctg tagccgagga
      541 tcgcgttgag cggcgtgcgc agctcgtggc tcatgttcgc cacgaacgcg ctcttggccg
      601 cgctcgcctc ctccgccagc cgcgtcgcct cctgcagctg cgccgtgcgc tcgccgagct
      661 gctcggcgag cttgcgcgcg tcggtcagcg aggtccgcac cgtgttcatc accatcagga
      721 tgtcggtcgt cgcgtcgtgc agcgcgaagt ccttgaagtg cagcccgagc tgctgcagct
      781 gcccgagctc ggtgatccac ggcgtgccga ggaacagcag ctcctcgccg accgccagca
      841 cctgaccgcg cagcttgaac gtccgcgcgc gcgcctcgag gatgaacagg gtgtcggcgc
      901 gggccagcat cgccgcgacg ccggtgctgc gcagcatcgg ccgcgcgacc gtgaaggccg
      961 cgtcgaacgc gtcgccgacg aacacccccg gcagcagctt gcgcaggccc ggcccgagct
     1021 gcgtcagccg cccgctctgg tcgaccgcca gatggaacgg gaagacgcgt tcgatctggc
     1081 caggcatgag cgacagggcc atgtgcgatc agctcgcggc ggactccggc gcgggcgcgg
     1141 ccagctcgcg gatgtgaaac tcgtcgtggc cgacctccgc ccgtgacttc acgtgctcga
     1201 cctcgacatt tttattaaaa gtcgccgcca gccccttgag cagcccgatc atcatcggcg
     1261 ccaggccctc gcgggacgag aaatacgaca gcagatactc gccctcgccg aggtcctgac
     1321 agtcgaaggt cggcggtcgc agctgcgaga agctcaggcc gatgtgggtg tgcagcgcgt
     1381 cgaggctgtg gagaaactcg ccgaggctgc tcccgaacat cgagaacatc tcgccatagc
     1441 cctccttcgc cgtgtagcgg atccagaact cgccgaaggc ctcgagcacg gtcgccggcg
     1501 gcaggccgag cacctcgctg gcggccccga cgagcttgta tgtgacgtcg tccgggtagg
     1561 ggtccatgct gatgaagctg tcgaccccga tccccgcgcg ggtccggatc tggttccaga
     1621 catcggcgcc gaatcgggta caaaccaggt cttccacggc cttgttgacg agtccgtaca
     1681 tgcgtacccc cggctcaggg tcgcagatcc ccggcgagct tgcacatgtc ggcaagcgcg
     1741 cccggttcga ttttgctcgc gcgcgctatt tgcgggccgt gcgccgctcg gcgagctgaa
     1801 acagcaggac cccgagcagc accagcgcgc cgccggtcat cgtgatgatg ccgaggcgct
     1861 ccccgcgaaa cagcagcccg agcacgaccg cgatcggcgg cgtgatcagg gcgaccatcg
     1921 ccatcagcgt cgcgctcagc tgcttgagca ggcgatagta cacgaggaac gtgatgatcg
     1981 acccgaacac cgcgaggtac gcgatcgatc cgatcaccgg cagcgtccac gggaagggct
     2041 cgctccactc gccgcgcacg ccggccatca gcgccagcag cacgctgccg atcagcatgc
     2101 ccatgccgct gaggaacgcc gaggggacat tggccccgtc gcgtttgacg tagacctggc
     2161 cgaccccctg gatcagcacc gacgcggtga tgatcgctac ccccagtaag ctctccggcc
     2221 cgagctcgcg caggctgccg ctgtagagcg tgaacagccc cgcgaggccg gtggcgatcg
     2281 caccaatttt cccggccgtg aggcggtcgc cgaggatgaa gtgggccagc accgcgaccc
     2341 agaacgggaa caccccgaag gtcagcgagc ccagccccga gggtacgtac atgccgccga
     2401 tgtacgtaca cccgtacccg accccgaagc tgcacacacc gatcaagagc gcgagccgcc
     2461 aggcgacgcg gtcgcggaac gccgggcgcc cggtccacca cgcgagcggc accagcacca
     2521 gcgccgcgac cgcaaagcgc aggcagaccc cgagcagcgg cggcatgccg gcgtcgagcc
     2581 ccatcttgat gccgagccag gtcgagcccc agatcaggca cagcaccacg tagccgagcg
     2641 ccgtcaactg cttcgctccg cgcctggacc gtgcaaatac atcggcgcgg agtatagcgg
     2701 ccatgcatcg catgcccgcc ctaccccgcg ccgtcaacga gtgtggcgag tcaccacctc
     2761 ggcggacgcc tgaccgcccg tgcgatcacg gcgcgcagta cgggccgtcc gcggcccaac
     2821 cagcgacggg caccgtcagc gtgtggccgt tgccgctcat gtcctgcgac atcaaacccg
     2881 tgccctcggg cgtcgggtag aacgcgagcg gggccggcgg gtacacgccc tcgaccaggc
     2941 ccatcacgtc ggcctcgccg agcgccgtgc cgtagacgac gacatcggtg atgtcggcct
     3001 tcgaggcgta gaacagctgg cccatcagcg gctcggcgcc gatgctaaac ggcagcgccg
     3061 ggttcgcggc gaccaggccc atgcccttct tctcgctgtc gagcacgacg ccgtcgcggt
     3121 agagcacgcc gttgccggtc gccgcgtcgt aggtgagcgc gtagtggtgc cacatgccgt
     3181 cggacttggc catcggggcg gtgaggtcga ggaaggcctc caggccgacc tgcagccgcg
     3241 cggtcgtgcc ggtcatgccg gtggcgatcg cgtacgtcga gtggccgccg acatccttgc
     3301 ccgccttgac gaagatcacc ccgtacgggg cgttcgccgc cgccttgtgc cagcccgccg
     3361 ccgtcagcga caccgtgtgc agcttcaccg ggtcggccgc ctgcgcgagc gtcttgccct
     3421 cgaagtgcag cgccgtgcac tcctgcttgc acgccgacgt gcagccgtcg ccgaggtcgt
     3481 ggtcggcgtc gtcgcaggct tcgtccgcgt cgacctcgcc gttgccacac accgcatcga
     3541 cacagtacgg gccgtccgcg gcccagccac cgtcgggcac cgtcagcgtg tgaccgttgc
     3601 cgctgacgtc ctcggacatc gcgcccatgc cctcgggggt cgggtagaac gcgagcgcga
     3661 gcggcgggta cacgccgtcg atcagcccct tgacctcgcc ggcggtcagc gccgtgccat
     3721 agacgacgac atcggtgatg tcggccttcg aggcgtagaa cagcttgccc atcagcggct
     3781 cggcgccgat gctaaacggc agcgccgggt tcgcggcgac caggcccatg cccttcttct
     3841 cgctgtcgac gacgacgccg tcgacgtaga gcacgccgtt gccggtcacc gcgtcgtagg
     3901 tgagcgcgaa gtggtgccac ttgccgtcgg gcttgaccgt cggcgcggtg aggtcgagga
     3961 aggcctcgag gccgacttgc agccgcgcgg tcgtgccggt gttgccggtg gcgaccgagt
     4021 acgtgacgtg accgccgacg tccttgcccg acttggtgaa aatcgacccg tacggggcag
     4081 tcgcgtccgc cttgtgccag cccgccgccg tcaccgatat cgtgtgcagc gtcgtcgggt
     4141 tggccgcctg cgcgatcgtc ttgccctcga agtgcagcgc cgtgcactcc ctggtgcact
     4201 ccgccgtgca accgtcgccg aggcccgcgt tggcgtcgtc gcacgcctcg tcctgctcga
     4261 cctcgccgtc gccacacacc gcgtccgcgc cggtgctgct cgaacccccg gtcgtgtccg
     4321 tcgtgctcgt cgaagcatcc gtcgtgctcg tcgaaacgtc cgtcgtgctc gccgtgtcgg
     4381 tcgtgtccgt cgttccgacc gggtcggtcg tcgaaccggt cgtgctgctc gacgtcggcg
     4441 cgtccgtggt gctggtgtcg ccggtcgtgc cgcccgtcgg ctcgatgtca cccgtcgtgc
     4501 cggtcgtcgt gccgacatcg gtggtgctcg atgtcgtcgt cgtggtctcg ccgccacccg
     4561 ggcaggccgt gagcgccagg accgtcgcca gagcgaccgt cttaccggga ttgacgggac
     4621 cgacgacccg agagtacgtg gagaacttca tatcaacttc cttttgcaca aactacgtat
     4681 atcgaggcga acgcgaggga atctcccctc gcgcgcatcg acgtgaatcg ccgaatctca
     4741 cgccacaacg accctgtcaa gccgcatatt cgccacttcc aaagcggtgg gcgcccgccc
     4801 agcgcccgcc cagcgcccct tccacgcggc ttcccgaatc tccgtgtcac attgcacgcg
     4861 cagcgctcat ttcctcgcac gatcgccgcg ccggcagcgt ctcgagcagt gaggctgtcc
     4921 gcccccgtcc atcgatacgc ggcaaacatg gcgcgattga tcgccacgcc gtcgggcggg
     4981 ccgcgcgcca ggcgtcgcgg cgcgatccac agggactcgc ctgaccgccc aggcggtcac
     5041 ggcgcgcaat acgggccgtc cgcgacccag ccgccgtcgg gcaccgtcag cgtgtggccg
     5101 ttgccgctca tgtcctgcga catcaaaccc gtgccctcgg gcgtcggata gaacgcgagc
     5161 ggagccggcg ggtacacgcc ctcggccaga cccatcacct cggcctcgct cagcgccatg
     5221 ccgtagacga cgacatcggt gatgtcggcc ttcgaggcgg cctcgaggtt gccctgcagg
     5281 atcccggagc cgatgctaaa cggcagcgcc gggttcaccg caaccaggcc catgcccttc
     5341 ttctcgctgt cgagcacgac gccgtcacgg tagagcacgc cgttgccggt cgccgcgtca
     5401 taggtgaccg cgtaatggtg ccacgtgccg tcggacttcg ccgtcggcgc ggagaggtcg
     5461 atgaagatcg cggggccgac ctgcagccgc gcggtcgtgc cgggcatacc gttggcgatc
     5521 gtgtacgtga tgtgcccgcc gacgtcctga cccgccttgc tgacgatcgc accgtagggg
     5581 gcgttcgccg ccgccttgtg ccagcccgcc gccgtcaccg acaccgggct cagcgtcgcc
     5641 gggttggccg cctggaccgc cgtcttgccc tcgaagtgca gcgccgtgca ctccttcgtg
     5701 cacgccgatg tgcaaccgtc gccgaggttg taattggcgt cgtcgcacgc ctcgtccatg
     5761 tcgacctcgc cgttgccgca cgacctgtcc gcgcagtacg gggcgtcatc gacccagccg
     5821 ccgtcgggca ccatcagcgt gtggccgttg ccgctgacgt cctcggacat cgcgcccatc
     5881 ccctcgggcg tcgggtagaa cgcgagcgcg agcggcgggt acacgccctc gaccagcccc
     5941 ttcacctcgt cggcggtcag cgccgtacca tagacgacga catcggtgat gtcggccttc
     6001 gacgcggcct cgaggttgcc ctgcaagatc ccggagccga tgctaaacgg cagcgccggg
     6061 ttcgtcgcga ccaggccctt gcccttgatc tcgctgtcga tgacgacgcc gtcgcggtag
     6121 agcacgccat tgccggtcac cgcgtcatag gtgaccgcgt aatggtgcca cgtgccgtcg
     6181 ggcttgaccg tcggcgcggt gaggtcgagg aaagccaggg gaccgacctg cagccgcgcg
     6241 gtcgtgccgg gcatgccatt ggcgatcgtg taggtgatgt gccc
//
LOCUS       DASWNJ010001143         1838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_332875, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001143 DASWNJ010000000
VERSION     DASWNJ010001143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332875"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VGB85_19310"
     CDS             <1..239
                     /locus_tag="VGB85_19310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136246.1"
                     /translation="LIEVGRADNITATGTIFAVFGTLVQAAGLSMGIAGTVLFVRDGK
                     AQQARQAGLHLGRGVHLRALPRLDGATMNLSYRF"
     gene            complement(355..1422)
                     /locus_tag="VGB85_19315"
     CDS             complement(355..1422)
                     /locus_tag="VGB85_19315"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0136247.1"
                     /translation="MKTDERTRRILDAAVQLAEAGGFAAVRLRDVAQTSGVALGTVYK
                     RFRSKEDLLMGVLSHELHDLRTQLADSPVCGPTPLVRVIGFFGFLTEFLCRRPNLARA
                     VVRSAASGEKALSERLASFNAALFELSFSAMQGGPGRAPSPGEAVMLPALIQVWFSAL
                     CGWAGNVYDRSGVVEQVAAAARLMTHGVDRMPANTSEPRAPLRVLVRGAGRPSLSRDE
                     VVVELVSAAGPGVVGELLARPELDVLQLDGPIDEVDIGEAPAWTGTGRPRIVVITRAT
                     DAALLRRLAGHVDVVLTATGLPAGAGETLTTTLYLALARGASLQDAHDEAREAIAPVA
                     GDAQIALTTRPGADLRALSLR"
     gene            complement(1464..>1838)
                     /locus_tag="VGB85_19320"
     CDS             complement(1464..>1838)
                     /locus_tag="VGB85_19320"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0136248.1"
                     /translation="DVIAPVELGGHRVEAGELVIYLPYFTHRHPDFWDEPERVKPERF
                     TPEAMQGRHKCAYVPFSAGPRMCIGNHFSLLEGALILAMISQRYRFTLSPRARIEPQF
                     QITMRPKYGVLLTVAERRAGGG"
BASE COUNT          263 a          691 c          633 g          251 t
ORIGIN      
        1 cgctgatcga ggtcggccgc gccgacaaca tcaccgccac tggcacgatc ttcgccgtgt
       61 tcgggaccct cgtgcaggcc gcgggcctgt cgatgggcat cgccggcacc gtcctgttcg
      121 tccgcgacgg caaggcccag caggccaggc aagccggcct gcacctcggc cgcggcgtcc
      181 acctgcgcgc cctgcctcgc ctcgacggcg cgacgatgaa cctgtcgtac aggttctgag
      241 acccgtcgcc gcgtgagctc gaagggacat gtcctcgcgg acatgtccct tcgttgcgca
      301 gcgctcaccc gtaggaccgc caggcgcacc ggcctgttcg tggtcgtcaa cccgtcagcg
      361 cagcgacagc gcccgcaggt cggcgccggg gcgggtcgtc agggctatct gtgcgtcgcc
      421 agcgaccggg gcgatcgcct cgcgggcctc gtcgtgggcg tcctgcagcg aggcgccgcg
      481 cgcgagcgcg aggtacagcg tcgtcgtcag ggtctcgccg gcgcccgcgg gcaggcccgt
      541 cgcggtgagc accacgtcga cgtgacccgc gagcctgcgc aggagcgccg cgtcggtcgc
      601 tctggtgatc acgacgatcc gcggccgtcc ggtgccggtc cacgcgggcg cctcgccgat
      661 gtcgacctcg tcgatcgggc cgtcgagctg caacacgtcg agctcgggcc gcgcgagcag
      721 ctcgccgacg acacctggcc ccgcggcgga cacgagctcg acgaccacct cgtcgcgcga
      781 cagcgacgga cgacccgcgc cacgcaccag cacccgcagc ggcgcgcgtg gctcgctcgt
      841 gttcgccggc atgcggtcga cgccgtgggt catcaggcgc gcggcggcgg cgacctgctc
      901 gaccaccccg ctgcggtcgt aaacgttgcc ggcccagccg cacagcgccg agaaccacac
      961 ctggatcagc gcgggcaaca tgaccgcctc gcctggcgag ggtgcgcgtc cgggtccgcc
     1021 ctgcatcgcc gaaaaactga gctcgaacag ggcggcgttg aagctcgcca ggcgctcgct
     1081 gagcgccttc tcgccggacg cggccgatcg caccaccgcg cgcgcgaggt tcgggcggcg
     1141 gcacaggaat tcggtcagga atccgaagaa gccgatcacg cggaccagcg gcgtcgggcc
     1201 gcacaccggc gagtcggcga gctgcgtgcg caggtcgtgc agctcgtggc tgagcacgcc
     1261 catcagcagg tcttccttgc tgcgaaaccg cttgtacacc gtaccgagcg cgacgcccga
     1321 cgtctgggcg acgtcgcgca ggcgcaccgc cgcgaagccc cccgcctccg ccaactgcac
     1381 cgcggcgtcg aggatccgcc gcgtgcgctc gtcggtcttc acgacctcca tcctcccggt
     1441 gtaccggccc ggggcggccg cggtcaaccg ccgcctgccc gccgctccgc caccgtcagc
     1501 aaaacgccgt acttcgggcg catcgtgatc tgaaattgcg gctcgatccg cgcgcgcggg
     1561 ctcagcgtga agcgatagcg ctggctgatc atcgccagga tcagcgcgcc ctccagcagc
     1621 gagaagtggt tgccgatgca cattcgcgga ccggcgctaa acggcacgta tgcgcacttg
     1681 tgccgcccct gcatcgcctc cggcgtgaag cgctccggct tgacgcgctc cggctcgtcc
     1741 cagaagtcgg ggtggcggtg cgtgaagtac ggcaagtaga tcacgagctc gccggcctcg
     1801 acgcggtggc cgccgagctc gaccggcgcg atgacgtc
//
LOCUS       DASWNJ010001144         2508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_657054, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001144 DASWNJ010000000
VERSION     DASWNJ010001144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657054"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VGB85_19325"
     CDS             complement(<1..594)
                     /locus_tag="VGB85_19325"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0136249.1"
                     /translation="MTTIEPTPAPRPLEFQRMFAQVRAGIFGASEPVRVGRYVLRGRL
                     GVGGMGVVYAADDADLGRRVAIKLLHGTGSQDQARGRILREAQALARLSHPNVVQIYE
                     VGEVGRQVFVAMELVEGLTLAQWQRQQTRPWRELLEVYVQAGRGLAAAHDVGLVHRDF
                     KPENVLVGADGRVRVVDFGLARVAGEAPIDPRRPSGDA"
     gene            668..1417
                     /locus_tag="VGB85_19330"
     CDS             668..1417
                     /locus_tag="VGB85_19330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136250.1"
                     /translation="MTSSSSSTEHSVLIALRELDSLEQQRRDELAARERAELAARAAA
                     KAEAEAHIRAEQAARARAIAEAEAEVARRAHALHLRNLEIEAEVQRERDARLLRVQAE
                     IDGQLRSHSRRATAGQRLVGAVLLAILGLAGAIGATIVTRPQASVVEHAVAGANDLRH
                     MAALKEYAATVAAIEQDLGRLRSDNERQEAVVEAAAALRAMLQARPEEPARPVARPVK
                     PRPQTPPGAGKKPTSGQIKICKSDDPLAEDC"
     gene            1648..>2508
                     /locus_tag="VGB85_19335"
     CDS             1648..>2508
                     /locus_tag="VGB85_19335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136251.1"
                     /translation="MLTLTFALSAARRGALVTGLGASLVACSGGGAETTESSGTTENA
                     AASTDTTTSPTGTSTGTGTSTGTSTDPSTGVDEPTTTLDSGDTTSPVMTEPMSGTSTS
                     TGTTTDDTTGDSTGSSTGEAMDSSTSSSTGEAMDTSTGEPAQEVVLSVGPLECKEHAV
                     IFTTVPEEAGHYVAATLTPEAYPVTVERVKYLMRGPGYDPACTTAIAHDVQVFVSLTP
                     KPQAKPSETAKSFVQIPVPAEAMTEQFRTVELVLPQPIELVEGERLVVAVQLAATQDL
                     ASMLCLRSCFD"
BASE COUNT          393 a          943 c          868 g          304 t
ORIGIN      
        1 cgcgtcgccg ctcggccggc gcgggtcgat cggcgcctcg ccggcgacac gcgcgagccc
       61 gaagtcgacg acgcgcaccc gaccatcggc gccgacgagc acgttctcgg gcttgaagtc
      121 gcggtgtacc aggccgacgt cgtgcgccgc cgccagcccg cgtccagcct gcacgtacac
      181 ctcgagcagc tcccgccacg gccgcgtctg ctggcgttgc cactgcgcga gcgtcagccc
      241 ctcgacgagc tccatcgcca cgaacacctg gcgcccgacc tcgccgacct cgtaaatttg
      301 cacgacgttc gggtgcgaca gccgggcgag cgcctgcgcc tcgcgcagga tccgcccgcg
      361 ggcctgatcc tgcgagccgg tgccatggag gagcttgatc gccacgcgcc ggccgaggtc
      421 ggcgtcgtcg gccgcgtaca cgacccccat gccgccgaca ccgaggcgcc cgcgcagcac
      481 atatcgaccc acccgcaccg gctcgctcgc gccgaagatc cccgcccgca cctgcgcgaa
      541 catccgctgg aattccagcg gacgcggcgc gggtgtcggc tcgatcgtgg tcatcgcggg
      601 ccgggacacg aacgccgtga tggttcgctt cggtctggac actcctatcg cacaaggcag
      661 atccaacatg acttcatctt catcgtccac cgaacactcc gtgctcatcg ccctgcgcga
      721 gctcgacagc ctggagcagc agcgccgcga cgagctcgcc gcccgcgagc gcgccgagct
      781 ggcggcccgc gccgccgcga aggccgaggc cgaggcccac atacgcgccg agcaggccgc
      841 gagggcccgc gcgatcgccg aggccgaggc cgaggttgcc cgccgggccc acgcgctgca
      901 cctgcgcaac ctcgagatcg aggccgaggt ccagcgcgag cgcgacgccc ggctgctgcg
      961 cgtccaggcc gagatcgacg gccagctgcg cagccacagc cggcgcgcga ccgccggcca
     1021 gcgcctcgtc ggggccgtgc tgctggcgat cctcgggctc gccggggcga tcggcgcgac
     1081 gatcgtgacc cgcccgcagg cgagcgtcgt cgagcacgcg gtcgccggcg ccaacgacct
     1141 ccggcacatg gcggccctga aggagtacgc cgccacggtc gcggcgatcg agcaggacct
     1201 cgggcgcctg cgcagcgaca acgagcgcca ggaggccgtg gtcgaggcgg ccgcggccct
     1261 gcgcgcgatg ctgcaggcgc ggcccgagga gccggcccgg cccgtcgcgc gtccggtcaa
     1321 gccgcgcccg cagacccctc ccggcgccgg caagaagccg acctccggcc agatcaagat
     1381 ctgcaagtcc gacgacccgc tcgccgagga ctgctgaccc tcaggacatg ccccaagcgc
     1441 caggttccgt cccgaggcgg gcgcccagct cggcggcgcg gtcatcgagc cgctcgaccg
     1501 tcgagcggcg tggcctgtgg tgccacagat ctcgcaatgt gtggcctgcg aagcgacgac
     1561 cggcgccgag actcgccccg gatcggcgct gcgcagctgc gggtcgctcc gcggggttgg
     1621 catacactgt gcacatggcg tttctcaatg cttaccttga ccttcgccct gtctgccgcc
     1681 cgtcgcggcg ccctcgtaac cggcctcgga gcgtcgctcg tcgcgtgcag cggcggcgga
     1741 gcggagacga ccgagagctc gggcaccacc gagaatgccg ccgcaagcac ggatacgacc
     1801 acgtcgccga cgggaacgag cacgggcacg ggcacgagca ccggcaccag caccgacccg
     1861 agcactggcg tcgacgagcc gacgacgacg ctggattcgg gggacacgac gtcccccgtg
     1921 atgacggagc ccatgtccgg cacctcgacg agcaccggca cgacgacgga cgacacgacc
     1981 ggcgattcga cgggatcgag caccggtgag gcgatggact cgtcgacgag ctcgagcact
     2041 ggcgaggcga tggacacctc gacgggcgag ccggcgcagg aggtcgtgct gagcgtcgga
     2101 ccgctcgaat gcaaggagca cgcggtgatc tttacgaccg tcccggagga ggcggggcac
     2161 tacgtggcgg cgacgctgac gccggaggcg tatccagtca cggtcgagcg cgtcaagtac
     2221 ctcatgcgcg ggcccgggta cgacccggct tgcacgaccg cgatcgccca tgacgtccag
     2281 gtgttcgtat cgctgacgcc caagccgcag gcgaagccct cggagacggc gaaatcgttc
     2341 gtccagatcc cggtgccggc cgaggcgatg accgagcagt tccggaccgt cgagctggtg
     2401 ctcccgcagc cgatcgagct cgtcgagggc gagcggctgg tcgtcgcggt ccagctcgcg
     2461 gccacccagg acctcgcaag catgctctgt cttcgcagct gcttcgac
//
LOCUS       DASWNJ010001145         1886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_403331, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001145 DASWNJ010000000
VERSION     DASWNJ010001145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403331"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..1310
                     /locus_tag="VGB85_19340"
     CDS             228..1310
                     /locus_tag="VGB85_19340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136252.1"
                     /translation="MPRVRLLLSLLLLADCRPAASPAPRCPAAAGPVAGERAGPEQQR
                     RARGLAALDAGRFDLARAEFAAILAVTPDDLVARTLHDASTRALMEAQERAAESFAAA
                     TPVVVAEPPWRHTLVREVPVASSTPPQLVALPTSRDAGIDEAEWFRRHGLRLPEYEVP
                     NPMRGLPGNVPPAIAPSFGAHLLVQAIGHPDHTIAIYGPSYSGGRFVAVLDADARRAG
                     FLDFDRYRRAPAAPPASAEQHVIWAEARDGVLYISHGDDERRGDDTAYLSALDVTSGA
                     LRWRSDPGVASAATFVIDGGHILSGHGSATGPAHVFVLDRRTGLVVGRARLASAPEYL
                     FVQGDTLLVRSEATAHAFELRRNRAS"
     gene            1353..>1886
                     /locus_tag="VGB85_19345"
     CDS             1353..>1886
                     /locus_tag="VGB85_19345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136253.1"
                     /translation="MSFLRDDGKAILAREIVAFEARTAAELVIVVEPRVGHYLHIPLI
                     FGSLAALATLAFLLYGEPAFSLHWFLVDPVLVGLLVGYLGSRWPALERAFTPTLWRER
                     WVLRAARAAFTARGVADTRGRTGVLIHIAVAERMATVLADLGVRRAISDAAFAQGSAP
                     LLAAVARGASASALAPYI"
BASE COUNT          204 a          820 c          600 g          262 t
ORIGIN      
        1 ccagcggcac catcgcgccc gaccccccgg tcggctcgcc cgtcgtcggc tcgcccgtcg
       61 tcgtcgtcgt cgcctccgcc cccgtgtcat tgccgtcccc gccacaggcg cctgccagta
      121 cacactcgag cgtcagggcc agccggcgtc gcatgccgcg agcatacgcc agccgcgagc
      181 ccgcgacacg cggcgccccg cgacccattc gcggtatcct caccgccgtg ccgcgcgtcc
      241 gtctcctgct ctcgctgctg ttgctcgccg actgtcggcc ggccgcgagc ccggccccgc
      301 gctgccctgc cgcggcgggg ccagtcgctg gcgagcgcgc aggacccgag cagcagcgac
      361 gcgcccgtgg cctcgcggcg ctcgacgctg gccgcttcga cctcgcgcgc gccgagttcg
      421 cggcgatcct cgcggtcacc cccgacgacc tcgtcgcgcg cacgctccac gacgcctcga
      481 cccgcgcatt gatggaagcc caggagcgag ctgccgagag cttcgccgcc gcgacgccgg
      541 tcgtcgtcgc cgagccgccg tggcgtcaca ccctcgtccg cgaggtcccc gtcgccagct
      601 cgacgccgcc gcagctcgtc gcgctgccga catcgcgcga cgccgggatc gacgaggcgg
      661 agtggtttcg ccgtcacggc ctgcgcctgc ccgagtacga ggtccccaac ccgatgcgcg
      721 gcctgccggg caacgtcccc ccggcgatcg ccccgagctt cggcgcgcac ctgctggtcc
      781 aggcgatcgg gcaccccgat cacacgatcg cgatctacgg cccgagctac agcggcggcc
      841 gcttcgtcgc cgtgctcgac gccgacgccc gccgggccgg cttcctcgac ttcgaccgct
      901 atcgccgggc ccccgccgct ccccccgcct ccgccgagca acacgtgatc tgggccgagg
      961 cccgcgacgg tgtgctctac atctctcacg gtgacgacga gcgacgcggc gacgacaccg
     1021 cgtacctcag cgcgctcgac gtcacgagcg gcgccctgcg ctggcgcagc gaccccgggg
     1081 tcgccagcgc cgcgaccttc gtgatcgacg gcggtcacat cctcagcggc cacggctccg
     1141 ccaccgggcc cgcgcacgtg ttcgtgctcg accgccgcac cggcctcgtc gtcggccggg
     1201 cccggctcgc cagcgccccc gagtacctgt tcgtgcaggg cgacacgctc ctggtgcgca
     1261 gcgaggccac cgcccacgcc ttcgagctgc gccgaaaccg cgcctcgtga ggccgctggc
     1321 cctcggcccc gctttcgtcg acaatctccg cgatgtcctt tctgcgcgac gacggcaagg
     1381 cgatcctcgc gcgagagatc gtcgccttcg aggccaggac cgccgccgag ctggtcatcg
     1441 tcgtcgagcc gcgcgtcggc cactacctcc acatcccgct gatcttcggc agcctcgccg
     1501 ccctcgccac cctcgccttc ctgctctacg gcgagccggc cttctcgctg cactggttcc
     1561 tcgtcgaccc cgtgctcgtc ggcctgctcg tcggctacct cggcagccgc tggcccgcgc
     1621 tcgagcgggc cttcacgccg acgctgtggc gcgagcgctg ggtcctgcgc gcggccaggg
     1681 ccgccttcac cgcccgcggc gtcgccgaca cccgcggccg caccggcgtg ctgatccaca
     1741 tcgccgtcgc cgagcgcatg gccaccgtgc tcgccgacct cggcgtgcgc cgggcgatct
     1801 ccgacgccgc gttcgcccag ggcagcgccc cgctgctcgc cgccgtcgcc cgcggcgcgt
     1861 cggcctcggc cctcgccccc tacatc
//
LOCUS       DASWNJ010001146         1698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_90842, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001146 DASWNJ010000000
VERSION     DASWNJ010001146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90842"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1698)
                     /locus_tag="VGB85_19350"
     CDS             complement(<1..>1698)
                     /locus_tag="VGB85_19350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136254.1"
                     /translation="VFDAVEQADEATLAALIQIVRRPGWLRLPLLLVFHGEPQGAAAE
                     LLAAVRSSDAEAVVEGDAAPASETRAFEWRGLPPRVIRVLRAGALIGAGFDTRMIADL
                     LGIEPLEVLEQIQHAIDLGVPVEDRGEGRFSLPHGALTALSASLLPSLAQAWHRQLGR
                     LLGARVARDAAPPPPPPPGAARSRPQEQRVDDTRVADIIDLSTEVTDLSEETAPPAPA
                     KPPPVRRRTMMIAPVAPAASPPAQPTELDDPVVDALRAAEHMRLAGDLDAAAEHLCEA
                     ARRTAEMGAPQAAVQHAAAALTLLASLPDSANRRQTRLRALLELGRVQWQSAGFELGF
                     TLAQARVTLDAARAQLDDEAPLELAVELAQATAGVHFDLGDPQSLARAHAELAAASQM
                     LKAADDATGAACLLNDQAAVRMRIGDSRGAVELLRESRKFFEARGAGDPVAVRELAET
                     DHLFAKLPLQVRMRVGQEEAGYVMGLDFALAAERVYRELDDVRELARVWETMGRLELR
                     RCRVEQARQRLEAAVEAQTRIGDLMGLATTTEVLSQVLAWCGRNAEAIALLRDSVVF"
BASE COUNT          206 a          628 c          652 g          212 t
ORIGIN      
        1 tgaacaccac cgaatcgcgg agcagcgcga tcgcctcggc gttgcgtccg caccaggcga
       61 gcacctgcga caggacctcg gtggtggtcg ccaaacccat caggtcgccg atgcgggtct
      121 gcgcctcgac ggcggcctcg aggcgctgcc gggcctgctc gacgcggcag cgcctgagct
      181 cgagccgccc catggtctcc cacacgcgcg cgagctcgcg gacatcgtcg agctcccggt
      241 agacgcgctc ggccgcgagc gcaaagtcga ggcccatcac gtagccggcc tcctcctggc
      301 cgacccgcat gcggacctgc agcggcagct tggcgaacag atggtcggtc tcggcgagct
      361 cgcgcaccgc gaccgggtcg ccggcgccgc gcgcctcgaa gaacttgcga gactcgcgga
      421 gcagctcgac ggcgccgcgc gagtcgccga tccgcatgcg cacggcggcc tggtcgttga
      481 gcaggcaggc cgcgccggtc gcgtcgtccg cggccttcaa catctggctc gcggccgcga
      541 gctcggcgtg cgcccgggcc agcgactgcg ggtcgccgag gtcgaagtgc accccggccg
      601 tcgcctgcgc gagctcgacg gcgagctcga gcggcgcctc gtcgtcgagc tgcgcgcggg
      661 cggcgtcgag cgtcacgcgg gcctgcgcga gcgtgaagcc gagctcgaag cccgccgact
      721 gccactgcac gcgcccgagc tcgagcagcg ccctgagcct tgtctgccgc cggttcgcgc
      781 tgtccggcag gctggcgagc agcgtcagcg cggcggctgc gtgctgcacc gcggcctgcg
      841 gcgcgcccat ctcggccgtc ctgcgcgccg cctcgcacag gtgctcggcg gccgcgtcga
      901 ggtcgccggc gaggcgcatg tgctcggcgg ctcgcagcgc gtcgacgacc ggatcgtcga
      961 gctcggtcgg ctgcgcgggc gggctcgcgg ccggcgcgac cggtgcaatc atcatcgtcc
     1021 ggcggcgcac cggcggtggt ttggccggcg cgggcggagc tgtctcttcg gacaggtcgg
     1081 tgacctcggt cgagaggtcg atgatgtcgg cgacgcgggt gtcgtcgacg cgttgctcct
     1141 gcggtcggct gcgcgccgcg ccggggggag ggggcggggg tggagcggcg tcgcgggcca
     1201 cgcgtgcgcc gagcagccgg ccgagctggc ggtgccaggc ctgcgcgagc gagggcagca
     1261 gcgacgcgga cagggcggtc aacgctccgt gcggcagcga gaatcggccc tcgccgcggt
     1321 cctcgaccgg caccccgagg tcgatcgcgt gctggatctg ctcgaggacc tcgagcggct
     1381 cgatccccag caggtcggcg atcatgcgcg tgtcgaagcc ggccccgatc agcgcgccgg
     1441 cccgcaacac gcggatcacc cgcggcggca ggccgcgcca ctcgaacgcc cgcgtctcgc
     1501 tcgcgggtgc ggcgtcgccc tcgacgacgg cctcggcgtc gctgctgcgg accgcggcga
     1561 gcagctcggc ggcggccccc tgcggctcgc cgtggaacac tagcagcagc ggcaggcgca
     1621 gccagcccgg gcggcgaacg atctggatca gcgcggcgag ggtcgcctcg tcggcctgct
     1681 cgaccgcgtc gaacacga
//
LOCUS       DASWNJ010001147         5022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_332909, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001147 DASWNJ010000000
VERSION     DASWNJ010001147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332909"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3640)
                     /locus_tag="VGB85_19355"
     CDS             complement(<1..3640)
                     /locus_tag="VGB85_19355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136255.1"
                     /translation="MSTSTPTTATIEAVIRAHQPALTARLRAPMLTALASRLASAGSD
                     AGLTSLVLAVPRVVAALRDEHAATQRRLAAIEAEVHAETSPAGQQLALARALRREVRD
                     RRRRLGDLRALRRDFDLAALRERFAGELDDQETQVELALGFLGSAATAVAMDPPSATP
                     LRRADLPAFLLTYLTWRPRFTMRLAATTALQQLCDALGPVDPDGTIAAAMTARVRDPE
                     EHPWVQAAALAVLTAVTPAEGMRVCAEILRAPHTDDPDVIASRTSAPVEAPPEMASTS
                     WFDMDVLANSTGGMVGAGARINIEAQAAASASDVWACETDAAMSIEAPIVAADPVPPP
                     PRDFTGRRAFLLRRLVIDWLVTQLGGSHGAAATDLITEAGSAGDPSEHVRIGLCRAAV
                     AMPIGDTRGAAMLTALADPMRESSAVVRSWALQASADTPISEALLHRSLADETSVLVL
                     RIACVAAVQCVAAGQSSGILIRTLTTLAARNDRSAAVHEAAAAALEAIDRATDPARAA
                     WTARLAQALASTPPGKSTAIAVRDLPAPDHLPRDLASRERWLGRILADLTRDDWGVDA
                     DLRGSRVTLRRGDRWRPRLWRLLHELRSPSPNKRQAHSHTRGRVLRGSVRAHSGRLHE
                     VTSTGVPGERVHIDREGGWGRHLPLVDDLLGLPWLRPRPVQVVSSHGVCTMTWRHGLG
                     ARLLARTRIMTRYAWLSALRGESLAAVELAQRGRYVATLRSELGIDLEFTPHPKPRLA
                     LGTAELISGEATPPERTNPGERRTAPELQAARPLPEQLRALFPVDDGAALSLPAGEAE
                     PPASFASFLLAPGLLDWLWLHAPYFISVSANSQQALAYFLAGLIAFGLVAAYLKNRRM
                     ARDRAAIPLVIGGWGTRGKSGTERLKAGLLHGLGYRVFSKTTGCEAMFIHAPPLGPAR
                     EIYSFRPYGKATIWEQHTLLNLAARMHSQVFLWECMALSPAYVQILQHSWMKDDLSTI
                     TNAYPDHEDIQGPAGMDVATVISGFVPTRGRLVTSEINFLPLMRAAAERRGTRISALG
                     DLDGELLPSDLLALFPYEEHPRNIALVARMAVELGLDPTLAIVTMAEHVVPDLGVLKR
                     FGPARLRGRLLEFINGCSANERTGFVSNWRRTGCDKLDLAAEPDRYVITVVNNRDDRV
                     SRSQVFARVMVEDVAVDRHVVIGTNTQGLLGYVRDALADFLARQVIVAPEDLSSGTGG
                     PALAANRLE"
     gene            complement(3637..>5022)
                     /locus_tag="VGB85_19360"
     CDS             complement(3637..>5022)
                     /locus_tag="VGB85_19360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136256.1"
                     /translation="VARSRQPAEVVVLGPTTLAVEARGLVSGGAGPGIVHVSVAQGDG
                     PFAPRGTVALISEVDPRATPEAGRTISAGPARTEVISLPEPGPQRVLLRPDSGAVLVR
                     LQQRLDAEPTPVPRPPIRALDLGALVDPLAPIGLPRTDVPPVAGPPVPQRFGTVWSEV
                     RGGRDDIEDNDDLAQANQVRTRVGYARELLARRMWLGVAPELRWRDHTALAYGGALAL
                     QAVFPTAGLRTRLSAATLAQDYADGPAWMAEGALYIDRLTWIAPRWQVVPSIDLRYRH
                     QSLTALDVAIAMDPVNPRVFTQYTADHPFALRPGVELRYQPLQDLRMFVGADFVPNSD
                     FRGLDQANFRGGLVGVMALLRRVVPEFALAYEGSVRLKDADRGETFLQNRMHAGLGLG
                     MWAGQAARIVFGVGDTLYVSGPFPLRNVVEAWLRLDLVFGRALRDYGPLDMAFKPVRE
                     HRLWTGEGGAR"
BASE COUNT          659 a         1698 c         2056 g          609 t
ORIGIN      
        1 cctcgaggcg gttggcggcc agcgcggggc cccctgtccc actggacagg tcctcggggg
       61 cgacgatcac ctggcgggcg aggaagtcgg ccagggcgtc gcgcacgtag ccgagcaggc
      121 cctgggtgtt ggtgccgatg acgacgtggc ggtcgacggc gacgtcctcg accatgacgc
      181 gcgcgaacac ctgcgagcgg ctgacgcggt cgtcgcggtt gttgacgacg gtgatgacgt
      241 agcgatccgg ctcggcggcg aggtcgagct tgtcgcagcc ggtgcgccgc cagttggaga
      301 cgaagccggt gcgctcgttg gcggagcagc cgttgatgaa ctcgagcagg cggccgcgca
      361 ggcgcgcggg gccgaagcgc ttgagcacgc cgaggtcggg cacgacgtgc tcggccatcg
      421 tgacgatcgc cagggtcggg tcgaggccga gctcgacggc catgcgggcg acgagcgcga
      481 tgtttcgcgg gtgctcctcg taaggaaaca atgcgagcag gtcggagggt aggagctcgc
      541 cgtcgaggtc gccgagcgca gagatccggg tgccgcggcg ctcggcggcg gcgcgcatca
      601 gcgggaggaa gttgatctcg gaggtgacga ggcggccgcg cgtcggcaca aagccggaga
      661 tcacggtcgc gacgtccatg ccggcggggc cctgaatgtc ctcgtgatcg gggtacgcgt
      721 tggtgatcgt ggacaggtcg tccttcatcc acgagtgctg caggatctgc acgtaggcgg
      781 ggctgagcgc catgcactcc cacaggaaca cctggctgtg catgcgcgcg gcgaggttga
      841 gcagggtgtg ctgctcccag atcgtcgcct tgccgtaggg acgaaagctg tagatctcgc
      901 gcgccgggcc gagcggcggg gcgtggatga acatcgcctc gcagccggtc gtttttgaga
      961 acacccggta gccgaggccg tgcagcaggc cggccttgag gcgctcggtg cctgacttgc
     1021 cgcgcgtgcc ccagccgccg atcaccagcg ggatcgcggc gcggtcgcgg gccatgcggc
     1081 ggtttttgag gtaggcggcg accaggccga aggcgatcag gccggcgagg aagtaggcga
     1141 gcgcctgctg gctgttggcg ctgacgctga tgaagtacgg cgcgtgcagc cacaaccagt
     1201 cgagcagacc gggcgcgagc aggaacgagg cgaacgaggc cggcggctcg gcctcgccgg
     1261 cgggcaggct cagcgcggcg ccgtcgtcga cggggaacag cgcgcgcagc tgctccggca
     1321 gggggcgcgc ggcttgcagc tcgggcgccg tgcgccgctc gccggggttg gtacgctcgg
     1381 gcggggtcgc ctcgccgctg atcagctcgg ctgtcccgag ggccaggcgc ggcttcgggt
     1441 gcggggtgaa ctcgaggtcg atgccgagct cgctgcgcag cgtggcgacg tagcggccgc
     1501 gctgggccag ctcgacggcg gcgaggctct cgccgcgcag cgccgacagc caggcgtagc
     1561 gcgtcatgat gcgcgtgcgc gcgagcagcc gcgcgccgag cccgtgccgc caggtcatcg
     1621 tgcagacgcc gtggctgctg acgacctgca ccggacgcgg gcgcagccac ggcagcccga
     1681 gcaggtcgtc gacgagcggc agatggcgac cccagccgcc ctcgcggtcg atgtgcacgc
     1741 gctcgccggg cacgccggtg ctggtgacct cgtgcaggcg gccggagtgg gcgcgcacgc
     1801 tgccgcgcag gacccggccg cgggtgtgcg agtgggcctg gcgcttgttg ggcgaggggc
     1861 tgcgcagctc gtgcagcagc cgccacaggc gcgggcgcca gcggtcgccg cggcgcagcg
     1921 tcacccgcga gccgcgcagg tcggcgtcga cgccccagtc gtcgcgcgtc aggtcggcga
     1981 ggatccggcc gagccagcgc tcgcggctcg cgaggtcgcg tggcaggtga tccggcgcgg
     2041 gcaggtcacg cacggcgatc gcggtcgact tgcccggcgg cgtgctcgcc agcgcctgcg
     2101 cgagccgggc ggtccaggcc gcgcgggccg ggtcggtcgc gcggtcgatc gcctcgagcg
     2161 ccgcggcggc ggcctcgtgc accgccgcgg accggtcgtt gcgggcggcg agggtcgtca
     2221 gcgtgcggat caggatcccg ctgctctggc cggcggcgac gcactgcacg gcggcgacgc
     2281 aggcgatccg caggaccagc acggaggtct cgtcggcgag gctgcgatgc agcagcgcct
     2341 cggagatcgg cgtgtcggcc gaagcctgca gcgcccagct gcggaccacc gcgctcgact
     2401 cgcgcatcgg gtcggcgagc gcggtcaaca tcgcggcgcc gcgggtgtcg ccgatcggca
     2461 tggcgaccgc ggcgcggcac aaaccaatgc gcacgtgctc gctcgggtcg ccggccgagc
     2521 cggcctcggt gatcaggtcg gtcgcggcgg cgccgtggga tccgccgagc tgcgtcacca
     2581 gccagtcgat gacgagccgc cgcagcaaga aggcgcgccg cccggtgaag tcgcgcgggg
     2641 gcggcggcac cgggtcggcg gcgacgatcg gcgcctcgat gctcatcgcc gcgtcggtct
     2701 cgcaggccca cacgtcgctc gcggaggcgg ccgcctgggc ctcgatgttg atccgcgcgc
     2761 cggccccgac catgccgccg gtcgagttgg cgagcacgtc catgtcgaac cacgaggtcg
     2821 aggccatctc cggcggggcc tcgaccggcg cgctggtccg cgaggcgatc acgtcggggt
     2881 catcggtgtg cggggcgcgg aggatctcgg cgcacacgcg catgccctcg gccggcgtga
     2941 cggcggtgag cacggcgagc gccgcagcct gcacccacgg gtgctcctcg gggtcgcgca
     3001 cgcgggcggt catcgccgcg gcgatcgtgc cgtccgggtc gacggggccg agcgcgtcgc
     3061 acagctgctg caaggcggtg gtcgccgcca ggcgcatggt gaagcgcggg cgccaggtca
     3121 ggtaggtcag caggaacgcg ggcaggtcgg cgcggcgcag cggggtggcc gagggcgggt
     3181 ccatggcgac ggccgtcgcg gcgctgccga ggaagccgag cgccagctcg acctgggtct
     3241 cctggtcgtc gagctcgccg gcgaagcgct cgcgcagggc ggcgaggtcg aagtcgcgac
     3301 gcagggcccg caggtcgccg agccggcgcc ggcggtcgcg gacctcgcgg cggagcgcgc
     3361 gggcgagggc gagctgctgg ccggcagggc tggtctcggc gtggacctcg gcctcgatcg
     3421 cggcgagccg gcgctgggtc gcggcgtgct cgtcgcgcag cgccgcgacc acgcgcggga
     3481 cggcgagcac gaggctggtc aggccggcgt cgctgccggc cgaggccagc cgcgaggcga
     3541 gcgcggtgag catcggggcg cgcaggcggg cggtcagcgc cggctgatgc gcgcggatca
     3601 ccgcctcgat cgtggccgtc gtcggcgtgc tcgtgctcat cgcgcgcccc cctcgccggt
     3661 ccacaggcgg tgctcgcgga ctggcttgaa ggccatgtcc agcgggccat agtcgcgcag
     3721 ggcccggccg aacacgaggt cgaggcgcag ccacgcctcg acgacgttgc ggagcgggaa
     3781 cgggccggag acgtagagcg tgtcgccgac gccgaacacg atccgcgcgg cctggccggc
     3841 ccacatgccg aggccgaggc cggcgtgcat gcggttctgc aagaaggtct cgccgcggtc
     3901 cgcgtccttg agccgcaccg agccctcgta ggcgagcgcg aactcgggga cgacgcggcg
     3961 cagcagggcc atcacgccga cgaggccgcc gcggaagttg gcctggtcga gcccgcgaaa
     4021 gtcgctgttg gggacgaagt cggcgccgac gaacatgcgg aggtcttgca agggctggta
     4081 gcggagctcg acgccggggc gcagcgcgaa cgggtggtcg gcggtgtatt gcgtgaagac
     4141 cctggggttg accgggtcca tggcgatcgc gacatcgagc gccgtcagcg actgatgccg
     4201 gtagcgcagg tcgatcgagg gcacgacctg ccagcgcggc gcgatccacg tcaggcggtc
     4261 gatgtagagg gcgccctcgg ccatccacgc cgggccgtcg gcgtagtcct gcgccagggt
     4321 ggccgcagac agccgggtgc gcaagccggc ggtcgggaac acggcctgca aggccagcgc
     4381 gccgccgtag gcgagcgccg tgtggtcgcg ccagcgcagc tcgggggcca cgcccagcca
     4441 catgcgccgg gccagcagct cgcgggcgta gccgacgcgc gtgcgcacct ggttggcctg
     4501 ggcgaggtcg tcgttgtcct cgatgtcgtc gcggccgccg cggacctcgg accacacggt
     4561 gccgaagcgc tgcgggaccg gcggaccggc gaccggcggg acgtcggtgc gcggtaaacc
     4621 aatcggcgcg agcgggtcga cgagcgcgcc gaggtcgagc gcgcggatcg gcgggcgcgg
     4681 caccggggtc ggctcggcgt cgaggcgctg ttgcaggcgc acgagcacgg ccccgctgtc
     4741 gggccgcagc agcacgcgct gagggccagg ctcgggcagc gagatgacct cggtgcgcgc
     4801 cggtccggcg gagatcgttc gcccggcctc gggggtcgcg cgcgggtcga cctcggagat
     4861 cagcgcgacg gtgccgcgcg gcgcgaacgg gccgtcgccc tgcgcgaccg agacgtgcac
     4921 gatgccgggc ccggcgccgc cgctgacgag gccgcgcgcc tcgacggcga gcgtggtcgg
     4981 gccgagcacg acgacctccg ccggctgacg cgagcgggcc ac
//
LOCUS       DASWNJ010001148         4393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_231977, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001148 DASWNJ010000000
VERSION     DASWNJ010001148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231977"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VGB85_19365"
     CDS             <1..231
                     /locus_tag="VGB85_19365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136257.1"
                     /translation="DKADPWIGCAEGTCTTVTIREPPRTLSGIRNSTDIDECAPEQVV
                     DLSSEFERNNAQVGVLLRAPWADDTLGGHGDV"
     gene            218..511
                     /locus_tag="VGB85_19370"
     CDS             218..511
                     /locus_tag="VGB85_19370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136258.1"
                     /translation="MAMSEREIDPVDAELAELDARPPRRPRFVLTRHQVAIIIVAGYA
                     AIAVGIKLFLSYLASTQGVNDERDEQAAMLFALVMVVLCIKALINIYRPVKPF"
     gene            518..1120
                     /locus_tag="VGB85_19375"
     CDS             518..1120
                     /locus_tag="VGB85_19375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136259.1"
                     /translation="MSAAAKEKRGKPRQQALPRLMAAGPSLRQAYFELIRDAVFRIAI
                     PPAKELEFFIGFVFGVITMVAAALAINAFYAIPVPVVLLLLVLPWSVMSGVFFSRAIS
                     LPDRGIFGLRERVDMERADYKYRLKRAAIEKRRRQLRASGASNEETESQLADEEQAAM
                     KQYLAELDHIDSARKALESDEAHKMLGPGGERDDEDKTGK"
     gene            complement(1151..2377)
                     /locus_tag="VGB85_19380"
     CDS             complement(1151..2377)
                     /locus_tag="VGB85_19380"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEY0136260.1"
                     /translation="MSPSPEPRAYPRILGRHEGGRPGPRIVVFGGVHGNEPAGCEAML
                     RVFARITRERLPLRGQLWGLAGNLRALAAGRRYLDRDLNRRWIREDIARIVNTGPEAA
                     IEDNEQLELLEILAPMLSDAHEPIVFFDLHSTSGPAAPFTCMADVLRNRDIALALPIP
                     VVLGLEEVLDGSLLGYLVDLGHIGVAVEGGQHTDPTTIDSHESAVWIMLVAAGALDPE
                     HLPDLALRRDRLARLARGIPPVSEIRHRHVVRPIDEPFVMQPGFENFQKITRGQTVAQ
                     DAHGPIAAPIGGMMMLPRYQGLGDDGFFVAKPVSRLALGASAALRHLRLDRLVPLLPG
                     IRRHPIHHDQYIADPSVARSRVVDIFHLFGYRHIRTVGPDLVFARRAPSVDSLRQPPA
                     EVRAMATRGHGRRREG"
     gene            complement(2361..4286)
                     /locus_tag="VGB85_19385"
     CDS             complement(2361..4286)
                     /locus_tag="VGB85_19385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY0136261.1"
                     /translation="MGEQNISTQVDEEHLRNFMKALLDDVVALEELLQTDRFETGVRR
                     VGAEQEMFLVDRSLRPASVVTDVLERVTDPRFTTELARFNLEANLTPQVFNSSCLRRM
                     EDELNELIGMARDGARACGADVLLTGILPTLRRGDLGLDNMTPKPRYLELNRTLRRLR
                     GGDFHVLIRGIEDFETTHDNVMLESCNTSFQVHFQVSPFEFAKFYNLAQAITGPLLAA
                     AVNSPVLLKHRLWHETRIALFERSVDTRPTGRGDRGARPRVHFGDGWIREGVLEIFRV
                     VIARFRILIANTFDENPREVIARGGIPKLSALRMHNGTVYRWNRACYGFDGDKAHLRV
                     ENRVLPSGPTVLDEVANAAFFFGMMANLAEEPRRLEETMEFEHARTNFFNAARDGIQA
                     QMTWVGGKKSSAQELLLKELLPRAREGLKFVNVDSDDIDRYIGCLEDRVKTGKTGAQW
                     MLSSLHAMGEQGTADVRLRTLSTAMLNNQKTGAPGHTWDLAKIEGAPGHWRDSYQTAG
                     QFMSTDLFTVQSHDIVDLAANIMDWKHIRHLLVEDEAGRLVGLLSHRALVRLVARGTR
                     SGIGEHPTVAALMVKNPLTIGPEMPTLEVMKLMRERRVSCLPVVEGDKLVGVITEADL
                     IEVSSKLLESYLRESQP"
BASE COUNT          686 a         1359 c         1607 g          741 t
ORIGIN      
        1 gacaaggcgg acccctggat cggctgtgcc gagggcacat gcaccaccgt gacgatccgc
       61 gagccgcccc gaacattgtc cgggatcaga aactcgactg atatcgacga gtgcgccccg
      121 gagcaggtcg tggatctttc cagtgaattc gagcgcaata atgcccaggt cggggttctc
      181 ttgcgggccc cgtgggcgga tgatacgttg ggaggtcatg gcgatgtctg agcgcgaaat
      241 tgatcccgtc gacgccgagc tcgccgaatt ggacgcgcgt ccgccacgta gacctcggtt
      301 cgtcctgacg cgccatcaag tggccattat catcgtggcc ggctacgcag cgattgcggt
      361 gggcatcaag ctgttcttat cctatttggc ttccacacag ggagtcaacg acgagcgtga
      421 tgaacaagcc gcgatgttgt ttgctctggt catggtcgtc ctttgtatca aggcgctgat
      481 caatatctat cgtccggtca agccgttctg acggtatatg agcgccgcgg ccaaggagaa
      541 gcggggcaag cctcggcagc aggcgctccc gcggctgatg gccgcgggcc cctctctacg
      601 ccaagcgtac ttcgaactca tccgtgacgc ggtcttccgc atcgccattc cgccggcgaa
      661 ggaactcgag ttcttcatcg gctttgtctt cggtgtgatc acgatggtcg ccgcagctct
      721 ggcgatcaac gcgttctatg cgatcccggt ccccgtggtg ttactgttgt tggtgctgcc
      781 gtggtccgtc atgtccggtg tgtttttctc gcgagcgatc agtctgcccg accgcggcat
      841 cttcggcctt cgggagaggg tcgatatgga gcgtgccgat tataagtatc gcctcaagcg
      901 tgcagcgatc gagaagaggc gccggcagct gcgagcctcg ggagccagca acgaagagac
      961 cgaatctcag ttggccgacg aggagcaggc agccatgaag cagtacctcg ccgagctcga
     1021 ccacatcgat tcggctcgca aggcgctgga gagcgacgag gcccacaaga tgctcgggcc
     1081 tggcggtgag cgagacgacg aagacaagac cgggaagtga tcacgtaacc aggcccgacg
     1141 cctcgacgcc tcaaccttct cggcggcgtc cgtgccctcg cgtcgccatc gcgcgcacct
     1201 cggccggggg ctggcgcagg ctgtcgacgc tcggggcgcg gcgggcgaac acgaggtccg
     1261 ggccgacggt gcggatgtgg cgatatccga acaggtgaaa gatatcgacg acgcggctgc
     1321 gcgcgacgct ggggtcggcg atgtactggt cgtggtggat cgggtggcgg cggatgccgg
     1381 gcagcagcgg gaccaggcgg tcgagtcgga ggtggcgcag cgccgccgag gcgccgagcg
     1441 cgaggcgtga caccggcttg gcgacgaaga agccgtcgtc gccgaggccc tgatagcgcg
     1501 gcagcatcat catgccgccg atcggcgcgg cgatcgggcc gtgggcgtcc tgcgcgaccg
     1561 tttgcccgcg cgtgatcttc tgaaaattct caaagcccgg ctgcatgacg aagggctcgt
     1621 cgatcgggcg gacgacgtgg cggtggcgga tctccgagac cggcgggatg ccccgcgcga
     1681 ggcgggccag gcgatcgcgg cgcagcgcga ggtcgggcag gtgctcgggg tcgagcgcgc
     1741 cggcggcgac gagcatgatc cagaccgcgg actcgtggct gtcgatcgtc gtcgggtcgg
     1801 tgtgctggcc gccctcgacg gcgacgccga tgtggccgag gtcgacgagg taaccgagca
     1861 gcgagccatc tagcacctct tccaaaccga gcacgacggg gatcggcagg gcgagggcga
     1921 tgtcgcggtt gcgcaggacg tcggccatgc atgtgaaggg cgccgccggg cccgaggtgc
     1981 tgtgcaggtc gaagaacacg atcggctcgt gcgcgtccga caacatcggt gcgaggatct
     2041 cgagcagctc gagttgctcg ttgtcctcga tcgccgcctc gggtccggta tttacgatcc
     2101 tggcgatgtc ctcgcggatc catcgccggt tgaggtcgcg gtcgaggtat ctgcggcccg
     2161 ccgcgagggc gcggagattg ccggcgaggc cccacagctg cccgcgcagc ggcaggcgct
     2221 cgcgggtgat gcgggcgaac acgcggagca tcgcctcgca gccggcgggc tcgttgccgt
     2281 gcacgccgcc gaacacgacg atacgcgggc ccgggcgccc gccttcgtgg cgtccgagga
     2341 tgcgagggta ggcccgcggt tcagggctgg gactcacgca ggtagctctc gagcagcttc
     2401 gacgagacct cgatgaggtc ggcctcggtg atgacgccga cgagcttgtc gccctcgacg
     2461 accggcaggc acgagacccg gcgctcgcgc atgagcttca tgacctcgag ggtcggcatc
     2521 tccggcccga tcgtcagcgg gttcttgacc atcagggcgg cgacggtcgg gtgctcgccg
     2581 atgccgctgc gggtgccgcg ggcgaccagg cgcacgaggg cgcggtgcga cagcaggccg
     2641 acgaggcggc cggcctcgtc ctcgaccagc aggtgccgga tgtgcttcca gtccatgatg
     2701 ttggcggcga ggtcgacgat gtcgtgcgac tgcaccgtga acaggtcagt cgacatgaac
     2761 tgacccgccg tttgatagct gtcgcgccag tgcccgggtg cgccctcgat cttggcgagg
     2821 tcccaggtgt ggccgggcgc gccggtcttc tggttgttga gcatcgccgt cgagagcgtg
     2881 cgcaggcgga catccgcggt gccctgctcg cccatcgcgt gcaggctcga gagcatccac
     2941 tgcgcgccgg tcttgccggt cttgacgcgg tcctcgaggc agccgatgta gcggtcgatg
     3001 tcgtcgctgt cgacgttgac gaacttgagg ccctcgcgcg cgcgcggcag cagctccttg
     3061 agcagcagct cctgggcgga ggacttcttg ccgccgaccc aggtcatctg ggcctggatg
     3121 ccgtcgcggg cggcgttgaa gaagttggtg cgcgcgtgct cgaactccat cgtctcctcg
     3181 aggcggcgcg gctcctcggc gaggttggcc atcatgccga agaagaacgc cgcgttggcg
     3241 acctcgtcga gcacggtcgg gccggacggc agcacgcgat tttcgacgcg caggtgggcc
     3301 ttgtcgccgt cgaagccgta gcaggcgcgg ttccagcggt acacggtgcc gttgtgcata
     3361 cgcaacgcgg acagcttcgg gatcccgccg cgggcgatca cctcgcgcgg gttctcgtcg
     3421 aaggtgttgg cgatcaggat gcggaagcgg gcgatgacca cgcggaagat ctcgagcacg
     3481 ccctcgcgga tccagccgtc gccgaagtgc acgcgggggc gagcaccacg gtcgccgcgg
     3541 ccggtcgggc gggtgtcgac cgaccgctcg aacagggcga tgcgggtctc gtgccacagc
     3601 ctgtgcttga gcagcaccgg cgagttgacg gccgcggcga gcagcgggcc ggtgatcgcc
     3661 tgggcgaggt tgtagaactt ggcgaactcg aacgggctga cctggaagtg gacctggaag
     3721 ctcgtgttgc aggactcgag catcacgttg tcgtgggtgg tctcgaagtc ctcgatgccg
     3781 cggatcagga cgtggaagtc gccgccgcgc aggcgccgga gcgtgcggtt gagctcgagg
     3841 tagcgcggct tgggcgtcat gttgtcgagg cccaggtcgc cgcggcgcag ggtcggcaag
     3901 atgccggtca gcagcacgtc ggcgccgcag gcccgcgcgc cgtcgcgcgc catgccgatc
     3961 agctcgttga gctcgtcctc catgcgacgc aggcacgagc tgttgaacac ctgcggggtc
     4021 aggttggcct cgaggttgaa gcgcgcgagc tcggtggtga agcgcgggtc ggtgacgcgc
     4081 tcgagcacgt cggtgacgac ggaggcgggt cgcaggctgc ggtcgaccag gaacatctcc
     4141 tgctcggcgc cgacgcggcg gacaccggtc tcgaagcggt ccgtctgcag cagctcctcg
     4201 agcgcgacga cgtcgtcgag cagagccttc atgaagttgc ggaggtgttc ctcgtcgacc
     4261 tgcgtggaga tgttttgttc gcccatgtgc ttgcgagggg ctcctggccc cgatccgagc
     4321 gcaaggtaac ctgaccggcg cgggcgacaa agtgacttgc gcgggcgcgg cctggcggtc
     4381 tttgcgcggg ctc
//
LOCUS       DASWNJ010001149         2381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_90866, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001149 DASWNJ010000000
VERSION     DASWNJ010001149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90866"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VGB85_19390"
     CDS             <1..326
                     /locus_tag="VGB85_19390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136262.1"
                     /translation="ERELAVEPEERWPSMAVLLAQLADDHPAEALALGERAFKIYDAS
                     EGMLPGELDAHFHRARALIATGGDRTRALADAELARSETHKLGPGKLEALTEIEGWLT
                     EQTAK"
     gene            complement(331..1242)
                     /locus_tag="VGB85_19395"
     CDS             complement(331..1242)
                     /locus_tag="VGB85_19395"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY0136263.1"
                     /translation="MRSAGTIRDKLHARRFADYLLTRGITTRMDSGAEGFTVWVHDDD
                     RLAEVREELPRFIAAPADPRYDVSREAEALRVQARQVEEATQAAARRLAVSVPAAARV
                     GYMGTPLALALVAFCLVVAVYTQFGEPGSPGLNQLMIASVHRVGPGRIGWNGLEDIAN
                     GEVWRLLTPCFIHFGILHLMFNMMWLLDVGRQIEASRGTLALALLVLLIGVGSNYGQY
                     IVEDPQFGGMSGVVFGLFGYVLVMSRFAPSAGMMMSANNALLMGIWFIVCLTGVVGPI
                     ANTAHAVGLVLGLAFGGIGVWLHERRR"
     gene            1403..>2381
                     /locus_tag="VGB85_19400"
     CDS             1403..>2381
                     /locus_tag="VGB85_19400"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEY0136264.1"
                     /translation="MHRLLVLLALAACAPRLSPATSQTLAAALRPTPETDDPAAPLTD
                     LAGLRDIVGAARIVGLGGPGPGARELTRVHHRVLRLLVEETGFTGLALDADATATLAL
                     DAFVRGAAVDLDAALLALADRGLATTEMRALLQYLRTYNAAHGGALRVFGLAPSDPEA
                     AAALALAYLERVDPAYVPKARSLLAGEQQFGVDAVRARLDARRADYLARSDPEAWATA
                     RQQVELVAQARRMVETWEFEAGEFARARNAEWALAQLGAGGKLVILADNRRVAKEVPG
                     AAPAMGDFLRQWFNNDYRAIAASVGDGSRLAARDPGTLCGTALPPPRAGS"
BASE COUNT          334 a          890 c          869 g          288 t
ORIGIN      
        1 tcgagcgcga gctcgccgtc gagcccgagg agcgctggcc gagcatggcc gtgttgctcg
       61 cgcagctcgc ggacgaccac cccgccgagg cgctcgcgct gggcgagcgc gcgttcaaga
      121 tctacgacgc cagcgagggc atgctgccgg gcgagctcga cgcccacttc caccgcgcgc
      181 gggcgctgat cgccaccggc ggcgaccgaa cgcgggccct cgccgacgcc gagctcgcgc
      241 gcagcgagac gcacaagctc ggccccggca agctcgaggc cctgaccgag atcgagggct
      301 ggctcaccga gcagaccgcg aagtgagcgc tcagcggcgg cgctcgtgca gccacacgcc
      361 gatgccgccg aaggcgaggc cgagcacgag gccgaccgcg tgcgccgtgt tggcgatcgg
      421 gccgaccacg ccggtcaggc acacgatgaa ccagatgccc atcagcagcg cgttgttggc
      481 ggacatcatc atgccggccg acggcgcgaa gcgggacatc accagcacgt agccgaacag
      541 cccgaacacg acgcccgaca tgccgccgaa ctgggggtct tcgacgatgt actggccata
      601 gttggagccg acgccgatca gcaggaccag cagcgcgagc gccagggtgc cgcgcgacgc
      661 ctcgatctgg cggccgacgt cgagcagcca catcatgttg aacatcaggt gcaagatccc
      721 gaagtggata aagcagggcg tcagcagccg ccagacctca ccgttcgcga tgtcctcgag
      781 gccattccag ccgatccgcc cggggccgac gcgatgcacg ctggcgatca tcagctggtt
      841 taggcctggc gacccgggct cgccgaattg cgtgtacacg gcgaccacca ggcaaaacgc
      901 gacgagcgcg agcgccagcg gcgtgcccat gtagccgacg cgggccgccg ccggcacgct
      961 gaccgcgagg cggcgggcgg ccgcctgggt cgcctcctcc acctgtcgcg cctgcacgcg
     1021 cagggcctcg gcctcgcgcg agacgtcgta gcgcgggtcg gcgggcgcgg cgatgaagcg
     1081 gggcagctcc tcgcggacct cggcgagccg gtcgtcgtcg tggacccaca cggtgaagcc
     1141 ctcggcgccg ctgtccatgc gcgtcgtgat cccccgcgtc aacaggtagt cggcgaagcg
     1201 ccgggcgtgg agcttgtcgc ggatcgttcc ggccgagcgc atggatcgcc ctgattaata
     1261 tcagagcccg gggccgcccg gggcccgggg ccgccgggaa tgcgcggccc ggcccgaagt
     1321 tgccgccgtg agtctcgccg gccctgcggg cccggccgtg gcccgccgac aggtcgatcg
     1381 cgcgtggtat accgcgcgac gcatgcatcg gctcctggtc ctgctcgcgc tcgcggcctg
     1441 cgccccgcgg ctgtcgcccg cgacctcgca gaccctcgcg gccgcgctgc gaccgacccc
     1501 ggagaccgac gaccccgccg cgcccctgac cgacctcgcg ggcctgcggg acatcgtcgg
     1561 cgccgcgagg atcgtcggtc tcggcgggcc agggcccggc gcgcgcgagc tgacccgcgt
     1621 gcatcaccgc gtgctccggc tcctggtcga agagacaggt ttcaccggcc tcgccctcga
     1681 cgccgacgcg accgccaccc tcgcgctcga cgccttcgtc cgcggcgccg cggtcgacct
     1741 cgacgccgcc ctgctcgcgc tcgccgaccg cggactggcg acgaccgaga tgcgggcgct
     1801 gctgcagtac ctgcgcactt acaacgccgc gcacggcggc gcgctgcggg tgttcggcct
     1861 ggcgccgagc gaccccgagg ccgccgccgc gctcgcgctc gcgtacctcg agcgggtcga
     1921 ccccgcgtat gtcccgaagg ccaggtcgtt gctggcgggc gagcagcaat tcggggtcga
     1981 cgccgtgcgc gcgcgcctcg acgcccggcg cgccgactac ctcgcgcgct ccgacccgga
     2041 ggcgtgggcg acggcgaggc agcaggtcga gctcgtcgcg caggcgcggc gcatggtgga
     2101 gacctgggag ttcgaggccg gggagttcgc gcgggcgcgc aacgccgagt gggcgctcgc
     2161 gcagctcggc gcgggcggca agctggtgat cctggcggac aaccgccgcg tggcgaagga
     2221 ggtcccgggc gccgcgccgg cgatgggcga cttcctgcgc cagtggttta ataatgacta
     2281 tcgcgcgatc gctgccagcg tcggtgatgg ctcgcggctc gcggcgcgcg acccggggac
     2341 gctgtgtggc accgccctgc cgccgccgcg cgccggcagc c
//
LOCUS       DASWNJ010001150         1769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_232001, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001150 DASWNJ010000000
VERSION     DASWNJ010001150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232001"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VGB85_19405"
     CDS             complement(<1..486)
                     /locus_tag="VGB85_19405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136265.1"
                     /translation="MRAGIRHLVACVSLVVLAGCAAAHSDGAADQAVTVPAADHHTHL
                     FTAAAIARLYRPPLPAVAVPPELARLLSERTRRWDDPAAIAELYAEDSVVFDFRGPRW
                     IRGRGEVAKYIGGLFMQAYHITPVILDIAGSRGHLVGYYATGEGSEQRPFGHVSLALQ
                     QG"
     gene            559..1482
                     /locus_tag="VGB85_19410"
     CDS             559..1482
                     /locus_tag="VGB85_19410"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0136266.1"
                     /translation="MTGAIDLRQLRSFLMLAEELHFGRAAQRLHIAQPALTQQIQALE
                     RAFGCALLERRPRVALTEPGQVLRREAQQTLARFDRGIAATLQVARGEAGPLAVGFSA
                     SAVLTHFPELVRQYHLRFPAVTLQLRELSPAAEVDSVRSGAVDVAFVRELADDPELVH
                     EVIVREPLAVLLPPRHPLARSPRVRIEALAREPFVHFPRETAPTLYDHIQAVCQRAGF
                     VPRVVQEVREWLTEISLVQAGIGVALVPASFKRLRLGGVSCRPLLGRAEHTTIALCYR
                     SAGLSPAAAGFIHMARELAARANRRGKPPLV"
BASE COUNT          229 a          641 c          624 g          275 t
ORIGIN      
        1 gccctgttgc agcgccaggc tcacgtgccc gaagggccgc tgctcgctgc cctcgccggt
       61 cgcgtaatag ccgacgaggt ggccgcgaga ccccgcgata tcgaggatca ccggggtgat
      121 gtgatatgcc tgcatgaaca ggccgccgat atacttcgcc acctcgccgc ggccgcggat
      181 ccaccgcggc ccgcggaagt cgaagacgac gctgtcctcg gcgtacaatt cggcgatcgc
      241 cgccgggtcg tcccagcggc gcgtccgctc gctcagcagc cgcgccagct ccggcggcac
      301 cgcgaccgcc gggagcggcg ggcggtacag gcgggcgatc gccgcggcgg tgaacaggtg
      361 ggtgtggtgg tcggccgccg ggaccgtcac cgcctggtcc gcggcgccgt cgctgtgggc
      421 cgccgcgcag ccggccagca cgacgaggga tacgcaggcg acgaggtgac gaattccggc
      481 tctcatgcgc gagagacttg ccacggcccg cgtcataaaa ccaatacctt gatctcaacc
      541 ggctgataag ttctgcttat gaccggggcg atcgacctgc gccagctgcg atccttcttg
      601 atgctcgccg aggagctgca ctttggccgc gcggcgcagc ggctgcacat cgcccagccg
      661 gcgctcacgc agcagatcca ggcgctcgag cgcgcgttcg ggtgcgccct gctcgagcgc
      721 aggccgcggg tggcgctaac ggagccgggc caggtgctgc ggcgcgaggc ccagcagact
      781 ctggctcgct tcgaccgcgg gatcgcggcg acgctgcagg tggcgcgcgg cgaggccggg
      841 ccgctcgcag tcggcttctc cgcgtccgcg gtgttgaccc acttcccgga gctcgtgcgg
      901 caataccatc tgcgcttccc agcggtgacg ctgcagctgc gcgagctgtc cccggccgcc
      961 gaggtcgaca gcgtgcgctc gggcgcggtc gacgtcgcgt tcgtccgcga gctcgcggac
     1021 gaccccgagc tcgtgcacga ggtcatcgtc cgcgagccgc tggccgtgct gctgccgccg
     1081 cgccacccgc tggctcgcag cccgcgcgtg cgcatcgagg cgctcgcgcg cgagcccttc
     1141 gtacacttcc cccgggagac tgcgccgacg ctgtacgatc atatccaggc ggtgtgtcag
     1201 cgggccggct tcgtgccgcg agtggtccag gaggtccgcg agtggttgac ggagatcagc
     1261 ctcgtgcagg cgggcatcgg cgtcgccctg gttccggcgt cgttcaagcg gctgcgcctg
     1321 ggcggcgtat cgtgccgtcc gttgctcggc cgggccgaac atacgacgat cgccctgtgc
     1381 taccggagcg cggggctgtc gcccgccgcc gccggcttca tacacatggc gcgcgagctc
     1441 gcggcgcggg cgaaccggcg cggcaaacct ccactggtct gaatcgcggc ggccggcgaa
     1501 cgagccgccg ccctcgtggt gtcctcgggc agccgcagtc tcccgcgatc gtgccggcct
     1561 cgcccttcac gcggagacac gaccgcaggg cgttgccgcg gatcagcggc ggacccacgt
     1621 gctgccgacc cgcgcgtact tctcgctgta ctgatcgtca tcggccggcg agacctcgcg
     1681 ctcgatcgac ccgtcgggcg cgatcgtcca cttttcgtcc cacttctcgt cgcggtcgag
     1741 gtcgatcttg aggcgattcc tgcgcactt
//
LOCUS       DASWNJ010001151         1762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_198148, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001151 DASWNJ010000000
VERSION     DASWNJ010001151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198148"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VGB85_19415"
     CDS             <1..964
                     /locus_tag="VGB85_19415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136267.1"
                     /translation="ASDTGDTTDTGAPGCGIVESLVIDDTTDLDALGCIEEVNGDLTI
                     GPTIALTSLAPLKNLRRVGGTLDIYDNAALVDLTGLDGLEEVGEWLIVRYNAALVTMD
                     GLGALTRLNALEVSSNDSMVALSSFGGAVEFGPWGYFELDRLPKLKSLKSLAGLKLIT
                     ADTTTIQIRVEQNQALTDLTGLGACCTGAHVDLAVLGNHALPSLAGLEPFTALGRLEI
                     VANHALKDLSGLGGVKTIEWLEIGMQCYDFPVEPSPVGNDSLVSLAGLNALTAVTMQL
                     NIMGNKKLPVAVANAFRDMVADKVGSEICDNQGGFSCESVLCPP"
     gene            complement(969..>1762)
                     /locus_tag="VGB85_19420"
     CDS             complement(969..>1762)
                     /locus_tag="VGB85_19420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136268.1"
                     /translation="DDELGLLELPDDGPARVLTLLPGALPDEHAVRKAHKPDFEALCR
                     WPGDGLLALGSGSTAARHRGCLLRPGARDMSDGTVLMIDMTPLHAHLAGAFPALNLEG
                     AAVLGDALVLLQRGNGPGNRSARVELDLAGAQQRIAADAPWDATLVRAIRDVELGERG
                     GAALGFTDASPLPDGSLVFVAAAEASPDTYQDGLVTGAAIGLMDPRGDVQWIVDFPEP
                     AKLEGIHAELAGDVLKLWLVTDPDDRARAADLYTAQVDRRTGAWR"
BASE COUNT          264 a          611 c          634 g          253 t
ORIGIN      
        1 cgcgagcgac accggcgaca cgacggacac gggcgcgccg gggtgtggga tcgtcgaatc
       61 gctggtcatc gacgatacca ccgacctcga cgcgctcggc tgcatcgagg aggtcaacgg
      121 tgacctgacg atcgggccga cgatcgcgct cacgtcgctg gctccgctca agaacctgcg
      181 cagggtcggc ggtacgctcg acatctacga caatgccgcg ctcgtcgacc tcacgggcct
      241 cgatgggctc gaggaggtcg gggagtggct catcgtccgc tacaacgccg cgctcgtgac
      301 gatggacggg ctcggggcgc tgacgcggct caacgccctc gaggtctcgt ccaacgactc
      361 gatggtcgcg ctgtcctcgt tcggcggcgc ggtcgagttc gggccgtggg ggtacttcga
      421 gctcgaccgg ctgcccaagc tcaagtcgct caagtcgctc gcgggcctga agctgatcac
      481 ggcggacacg acgacgatcc agatccgcgt cgagcaaaac caggcgctga ccgacctgac
      541 gggcctcggg gcgtgctgca ccggcgcgca cgtcgacctc gcggtgctcg ggaatcacgc
      601 gctcccgagc ctcgccgggc tcgagccgtt caccgcgctc ggtcggctcg agatcgtcgc
      661 caaccatgcg ctcaaggacc tctccgggct cggcggcgtc aagacgatcg agtggctgga
      721 gatcgggatg caatgttatg acttcccggt cgagccgagc ccggtcggca acgattcgct
      781 cgtcagcctc gccggactga acgcgctgac cgccgtgacg atgcagctca acatcatggg
      841 caacaagaag ctccccgtcg ccgtggcgaa cgcgtttcgc gacatggtgg cggacaaggt
      901 cgggtccgag atctgtgaca accagggcgg cttctcctgc gagagcgtgt tgtgcccgcc
      961 gtgagccctc agcgccacgc gccggtgcgg cggtcgacct gcgcggtgta gaggtcggcc
     1021 gcgcgggcgc ggtcgtcggg gtcggtgacc agccacagct tcagtacatc ccccgcgagc
     1081 tcggcgtgga tgccctcgag cttggcgggc tcggggaaat cgacgatcca ctgcacgtca
     1141 ccacgcgggt ccatgagccc gatcgcggcg ccagtgacga ggccgtcctg ataggtgtcg
     1201 gggctcgcct cggcggcggc gacgaacacc agcgagccgt ccggcagcgg ggaggcgtcg
     1261 gtgaagccca gcgccgcgcc gccacgctcg ccgagctcga cgtcccggat cgcacgcacg
     1321 agggtcgcgt cccacggcgc gtccgcggcg atccgctgct gcgccccggc gaggtcgagc
     1381 tcgacccgcg cgctgcggtt gccaggtccg ttgccgcgtt gcagcagcac cagcgcgtcg
     1441 ccgagcaccg cggcgccctc gaggttgagc gccgggaagg cgcccgcgag gtgggcgtgc
     1501 agcggggtca tgtcgatcat caggactgtc ccatcggaca tgtccctcgc gcccggtcgc
     1561 aagagacagc cgcggtggcg cgccgcggtc gagccggagc cgagcgcgag caggccgtcg
     1621 cccggccagc ggcacagcgc ctcgaagtcg ggcttgtgcg ccttacggac cgcgtgctcg
     1681 tccggcaatg cgccgggcag cagcgtaagt acccgcgcgg ggccgtcgtc gggcagctcg
     1741 agcaggccga gctcgtcgtc gg
//
LOCUS       DASWNJ010001152         1700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_198171, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001152 DASWNJ010000000
VERSION     DASWNJ010001152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198171"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1359
                     /locus_tag="VGB85_19425"
     CDS             <1..1359
                     /locus_tag="VGB85_19425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136269.1"
                     /translation="TAGTETTGDPTGSNTSGGPQTGLFACDSPTCTLLLVSQTLDDRV
                     DVFDVDSKTLRGRISLDLKPDASGQQIDGNLLDEPYELALTRTDLFVTVGHYPDTDQG
                     SLLRFPRDAFAALPAGGTFATAEFFNNVTFSAGVQGLAHGRREGIFLLPHNSGRLLVG
                     VFANNLQTSLWTTPSELLVIDPADFSAPPASVDLGALSVPCIGAWQMVALDEAMTRIA
                     VACDGSESVAVLTLPDDFGSAPLAEAVAGVTSCGFKLGGGDAWTTQFVAPNGAGGFLA
                     MQTQLAEAPRLWQLSGDCKPTSLPGKDVPPALQQVRLLRQPALLRPAGEGPPLWLVAS
                     ALPDPGVVVVRGGPTATMCGRLEGLGLLAAANAPWALALDAAGEHLAIGAGVPNNPEL
                     SEGKGQVLWATLDRSKEDSCEITATDVVDLNAGRYKISEPETWVRAPNVLVLAELTGG
                     GT"
     gene            1356..>1700
                     /locus_tag="VGB85_19430"
     CDS             1356..>1700
                     /locus_tag="VGB85_19430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136270.1"
                     /translation="MNQRSPRRVRLPVISAQAPTTEEDDPLLLLRGGRFARWFASGLA
                     IFGLAWIVGARVEAREADSVLVHATLQADPQVQALVQAMQAPPKPVVEDLPAAPPMPA
                     VEDITPPGPTPAM"
BASE COUNT          235 a          615 c          606 g          244 t
ORIGIN      
        1 accgccggca ccgagaccac gggcgacccg accggctcga acaccagcgg cggacctcag
       61 accggcctgt tcgcgtgtga cagtccgacc tgcacgctcc tgctcgtcag tcagacgctc
      121 gacgatcgcg tcgatgtctt cgatgtcgac agcaagaccc tgcgcggccg catctccctc
      181 gacctcaagc ccgacgcctc cggccagcag atcgacggca acctgctcga cgagccctac
      241 gagctggcgt tgacccgcac ggacctcttc gtgacggtcg ggcactaccc ggacaccgac
      301 cagggctcgc tgctccgctt cccgcgggac gccttcgcgg cgctgccggc gggtggcacc
      361 ttcgccaccg cggagttttt taataatgtg acgttctcgg cgggcgtgca gggcctcgcg
      421 cacgggcgcc gcgagggcat cttcctgctg ccgcacaact cgggccggct gctcgtcggc
      481 gtgttcgcca acaacctgca aacttcgttg tggaccacgc cgagcgagct cctcgtgatc
      541 gacccggccg acttttcggc gccgcccgcg agcgtcgacc tcggcgcgct gtcggtgcca
      601 tgcatcggcg cgtggcagat ggtcgcgctc gacgaggcga tgacgcggat cgcagtggcg
      661 tgtgatggca gcgagtccgt cgcggtgctg acgctgcccg acgacttcgg gtccgcgccg
      721 ctcgccgagg cggtcgcggg tgtcaccagt tgcggcttca agctcggggg cggtgacgcc
      781 tggaccacgc aattcgtcgc gccgaacggc gctggcgggt tcctggcgat gcagacccag
      841 ctcgccgagg cgccgcggct gtggcagctc agcggcgact gcaaaccgac gagcttgccc
      901 ggcaaggacg tgccgcccgc cttgcagcag gtccgcctgc tgcgccagcc ggcgctgctg
      961 cggcccgcgg gggagggccc gccgctgtgg ctggtcgcct cggcgctgcc ggatccgggg
     1021 gtcgtcgtcg tgcgcggtgg accgaccgcg acgatgtgcg gcaggctcga ggggctcggg
     1081 ttgctggccg ccgccaacgc gccgtgggcc ctcgcgctcg acgccgcggg tgagcatctg
     1141 gcgatcggcg ccggtgtgcc caacaacccc gagctcagcg agggcaaggg ccaggtgctg
     1201 tgggcgacgc tcgaccgcag caaggaggac agctgcgaga tcacggcgac cgacgtcgtc
     1261 gacctcaacg ccgggcgcta caagatcagc gagccggaga cctgggtgcg ggcgccgaac
     1321 gtactcgtcc tcgccgagct caccggaggt ggcacttgaa ccagcgaagt ccccgccgcg
     1381 tacgcctgcc cgtgatctcg gcgcaggcgc cgaccaccga agaagacgac cccctgctgt
     1441 tgctgcgcgg cggtcgcttc gcgcggtggt tcgccagcgg gctcgccatc ttcggcctcg
     1501 cgtggatcgt cggcgcgcgg gtcgaggcgc gcgaggccga ctccgtgctg gtacacgcga
     1561 cgctgcaggc cgacccgcag gtccaggcgc tcgtgcaggc gatgcaggcg ccgccgaagc
     1621 cggtggtcga ggacctgccg gccgcgccgc cgatgccggc ggtggaagac atcacgcccc
     1681 cgggaccgac cccagcgatg
//
LOCUS       DASWNJ010001153         2029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_90948, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001153 DASWNJ010000000
VERSION     DASWNJ010001153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90948"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="VGB85_19435"
     CDS             complement(<1..986)
                     /locus_tag="VGB85_19435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136271.1"
                     /translation="MRRAHGHFIVAAILILACACGPGRPRIADPTANVTTLPSPAPLT
                     TAELAILRKASAERGTLYWRVRHPDGPRCEAWTFEPDPDDESRGHLVHSDGDLRLRFT
                     HELSDGRLRLHSPERERVKLATPGALSTTIAALPCVFSGMSLRPAADPAPRQVVLTSD
                     ERWFLGAEACAAAGPDLDPQPPAPGEIHPLGCASALADPGTRTREDPPAPPDPPALRL
                     AASRRVFWLRHRGGRTVCEAWHHEAASPQRGSLRRDDAGSRTVYGYAAGLGVLTLIGP
                     NVARRVRGPDGPAELVRARGCLLTSAFTLADKVLSFGEDRWYLRRRDCERAR"
     gene            1126..>2029
                     /locus_tag="VGB85_19440"
     CDS             1126..>2029
                     /locus_tag="VGB85_19440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136272.1"
                     /translation="MNSRRKRLEREIDRDARVTTSGYVHEVMTVTLGRLQQVSLASQC
                     ADPVALFDEVDAITRKFRSFHRRLNDGRINRRGERDRHTPPPAELPAEVRPFAYHGDY
                     LGAFASVEAFAHACLDPSRPDQWADYVDLTQAGIDLHLSGRYWTVDANGVTHAFAVGT
                     PGPGGPDGPEGGPGDGEGEPAPSAPPTGPQVSSGAVTSSLPLHGRLLSLPGPRRVAGQ
                     PPLPRHEPERARAALVAAGCDEQTIAELMACYVAAYTTLGAHAWYWLRRGGVPARVLG
                     HTDLEALAADWCASGTLAWASDAEG"
BASE COUNT          270 a          714 c          804 g          241 t
ORIGIN      
        1 tggcgggcgc gctcgcagtc gcggcggcgc aggtaccagc ggtcctcgcc gaacgacagg
       61 accttgtcgg cgagcgtgaa ggcgctcgtc agcaggcagc cgcgcgcgcg caccagctcg
      121 gcggggccgt cggggccacg cacgcggcgc gcgacgttgg ggccgatcag cgtcagcacg
      181 ccgaggccgg cggcgtagcc gtagaccgtg cgactgccgg cgtcgtcgcg gcgcagagag
      241 ccgcgctgcg gcgaggcggc ctcgtgatgc cacgcctcgc agaccgtgcg gccgccgcgg
      301 tgacgcaacc agaacacgcg ccgactggcg gcgagtcgca gggcgggggg gtccggcggc
      361 gcgggcgggt cctcgcgggt gcgcgtgcca gggtcggcga gcgccgaggc gcagccgagc
      421 ggatggatct cgccgggcgc gggcggctgc gggtccaggt cgggcccggc ggcggcgcag
      481 gcctccgcgc cgaggaacca ccgctcgtcg ctggtcagca cgacctggcg cggggccgga
      541 tcggcggctg gccgcaagga catgcccgaa aagacacatg gcagcgccgc gatcgtcgtg
      601 ctgagcgcgc cgggggtcgc cagcttgacg cgctcgcgct cgggcgagtg caggcgcagg
      661 cggccgtcgc tcagctcgtg ggtgaagcgc aggcgcaggt cgccgtcgct gtgcacgagg
      721 tggccgcgcg actcgtcatc cgggtcgggc tcgaaggtcc aggcctcgca gcgcgggccg
      781 tcggggtggc gcacgcgcca gtacagggtg ccgcgctcgg cgctggcctt gcgaaggatc
      841 gcgagctcgg cggtcgtcag cggcgcgggc gacggcagcg tcgtgacgtt cgcggtcgga
      901 tcggcgatgc gcgggcgtcc gggcccgcac gcacacgcga ggatcaagat tgcagcgacg
      961 atgaagtgtc catgcgcgcg gcgcatcgcg gcggcgacgg taacgcagcg ggcgatcggc
     1021 ggccatcgct catcgcgtgg agaacagtcg cgtgaagcgt cgtgtgggtc ttgtgcgcgc
     1081 acccgagcag agagtatcgt gcggggcgga aaaggcactc gcatggtgaa cagccgcagg
     1141 aagcgactcg agcgggagat cgaccgcgac gcccgggtga ccaccagcgg ctacgtgcac
     1201 gaggtcatga ccgtcaccct gggccggctg cagcaggtca gcctggccag ccagtgcgcc
     1261 gacccggtcg cgctgttcga cgaggtcgac gcgatcacgc ggaagtttcg cagcttccat
     1321 cgccgcctca acgacggccg catcaaccgc cgcggtgagc gtgaccgcca caccccacca
     1381 cccgccgagc tcccggcgga ggtccggccg ttcgcgtatc acggcgacta cctcggcgcc
     1441 ttcgccagcg tcgaggcgtt cgcccacgcc tgcctcgacc ccagccgccc cgaccagtgg
     1501 gccgactacg tcgacctcac gcaggccggc atcgacctgc acctgagcgg ccgctactgg
     1561 accgtcgacg ccaacggcgt cacgcatgcg ttcgcggtcg gtacaccggg gcccggtggg
     1621 cccgatgggc cggagggggg acccggggat ggcgagggcg agccggctcc gagcgcgcca
     1681 ccgacgggcc cgcaggtgtc gagcggggcg gtgacctcgt cgctgccgct gcacgggcgg
     1741 ctgctgtccc tgccgggtcc gcgtcgcgtc gccgggcagc cgccgctgcc gcgccacgag
     1801 ccggagcggg cccgcgccgc gctcgtcgcg gccggctgcg acgagcagac gatcgccgaa
     1861 ttgatggcct gttatgtcgc cgcctacacg acgctcgggg cgcacgcctg gtactggctg
     1921 cgtcgcggcg gcgtgccggc gcgggtgctc gggcacacgg acctcgaggc gctcgccgcc
     1981 gactggtgcg cgtcgggtac gctcgcttgg gcgagcgacg cggagggca
//
LOCUS       DASWNJ010001154         3353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_657281, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001154 DASWNJ010000000
VERSION     DASWNJ010001154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657281"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1553
                     /locus_tag="VGB85_19445"
     CDS             <1..1553
                     /locus_tag="VGB85_19445"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HEY0136273.1"
                     /translation="DPTGATETGETGDDPPPAGVCDPPVQLVDVGAPDQVVGDGSAAS
                     CTEAALAVAVAAGGIVTFDCGAEPVTITLTQALAVVKDLVLDGGGTVTLSGGKATRIL
                     DMNTGNFEATGPLLTVQRLRFVDARSSGTQIPLGTDVDGGGGAIFHLGGSVTAVDCDF
                     ENNEAATLGPDVSGGAITGIGVGATVIVGCNFKNNRAANGGAVGGLHTALTIVNSTFT
                     GNQATGTGANYINEMGQQAGQGGNGGAIVMDGSGRPLTICGVTVAGNTGGAFGGALFR
                     TGYEGEPSVIDRSSFLDNEVPDHDDDALPSGAGGLYIQGTAVTMTATTVARNKARSAA
                     GLWVLGHGQQQARADLTNVTLADNETWPQRDFTERGIGGGLVIGDNTVGTLLNCTISG
                     NAAQFASGIGRVSDLTVRNTIISNIAENEYTPLNCTGSSFATPPGAGDHNLQWPNGQK
                     DDMDCTPGIVRVDPMLGALADNGGPTQTVSPLAGSPALAAGSECPTTDQRGMPRGEPC
                     TIGALEVQ"
     gene            1645..1893
                     /locus_tag="VGB85_19450"
     CDS             1645..1893
                     /locus_tag="VGB85_19450"
                     /inference="COORDINATES: protein motif:HMM:NF039376.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136274.1"
                     /translation="MSDTHSLDAGADATPLLIVDFGKRQKPKQIRKLRKGTGKLTEKI
                     QELVDELREQQTIAAGAQPVVIIVREKKKLMPRKLFGM"
     gene            complement(1933..2214)
                     /locus_tag="VGB85_19455"
     CDS             complement(1933..2214)
                     /locus_tag="VGB85_19455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136275.1"
                     /translation="MVRAIPASRSHCLRCDHDVEVEYHHSPAVRRWWKAYFYIPLMLL
                     PGLPFLAGDFAVSLPLMMAYMLGIGPALDIVRDPPLCATCGALIPRGRR"
     gene            complement(2282..2743)
                     /locus_tag="VGB85_19460"
     CDS             complement(2282..2743)
                     /locus_tag="VGB85_19460"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY0136276.1"
                     /translation="MSNTIPATETTWMKQARDLLMGAEPPPIARTIGFELVDIGSGSA
                     SFTLQADPARHANPMGTMHGGVLVDLGDAAMGFAMASTLREGESFTTIELKANYFKPV
                     WNARLRADAKMVKRSRSLGYLECDIVDDTGALVCRLASTCMVLRGDAASGR"
     gene            complement(2740..>3353)
                     /locus_tag="VGB85_19465"
     CDS             complement(2740..>3353)
                     /locus_tag="VGB85_19465"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0136277.1"
                     /translation="VKEALSKAERWAQTHTRIRQEAARLLRTRGLRLPSVAEVMAGAK
                     LTVGGFYGHWDSKEALFEEALRDSFRGNWAQLIEHARGDTTRERHAAIVRRYLSRAHR
                     DNPARGCPQPAAMGDAAALGGPYRDALAEEIEAIVADFARVAGPLGGKQLVLGLFALM
                     VGGLSLARATAGTPLSDAVLTASRALALAALEHAETARPKEQT"
BASE COUNT          531 a         1049 c         1312 g          461 t
ORIGIN      
        1 gtgatccgac gggcgcgacc gagaccggcg agactggcga cgacccgccg ccggcgggtg
       61 tgtgcgaccc accggtgcag ctggtggatg tcggcgcgcc ggatcaggtg gtcggggacg
      121 ggagcgcggc gagctgcacg gaggccgcgt tggcggtcgc ggtcgcggcc ggcgggatcg
      181 tcaccttcga ctgcggggcg gagccggtga cgatcacgct gacgcaggcg ctcgccgttg
      241 tgaaagatct ggtgctcgac gggggcggta cggtgacgct cagcggcggc aaggcgacgc
      301 ggatcctcga catgaacacc ggcaacttcg aggcgaccgg cccgctgctg acggtgcagc
      361 gcctgcgctt cgtcgacgcg cggtccagcg gcacgcagat cccgctcggc acggacgtcg
      421 acgggggcgg cggcgcgatc ttccacctcg gcggcagcgt gaccgcggtc gactgcgatt
      481 ttgagaacaa cgaggcggcg acgctcgggc ccgacgtgtc gggcggtgcg atcacaggga
      541 tcggcgtcgg cgcgaccgtc attgtgggtt gcaactttaa aaacaatcgc gcggcgaacg
      601 ggggggcggt cggggggttg cacacggcgc tgacgatcgt caacagcacg ttcaccggta
      661 atcaggcgac cgggaccggc gcgaattata ttaatgagat gggccagcag gcgggtcagg
      721 gcggcaacgg cggggcgatc gtgatggacg gctcggggcg gccgctgacg atctgcgggg
      781 tgacggtcgc gggcaacacc ggcggtgcgt tcggcggggc tttatttcgc accggctacg
      841 agggcgagcc gagcgtcatc gatcgcagca gcttcctcga caacgaggtg ccggaccacg
      901 acgacgacgc gctgccgagc ggggcgggcg ggttgtacat ccagggcacg gcggtgacga
      961 tgacggcgac gacggtcgcg cgcaacaagg cccgcagcgc ggcgggactg tgggtcctcg
     1021 ggcacgggca gcagcaggcg cgggccgacc tgaccaacgt gacgctcgcg gacaacgaga
     1081 cctggccgca gcgagatttt accgagcgtg ggatcggcgg cgggctggtg atcggcgaca
     1141 acacggtcgg cacgctgctc aattgcacga tctccgggaa cgcggcccag ttcgcgtcgg
     1201 ggatcggtcg cgtgtcggat ctgacggtgc gcaacacgat catcagcaac atcgccgaga
     1261 acgagtacac gccgctcaac tgcacgggca gcagcttcgc cacgccgccg ggcgcgggcg
     1321 atcacaactt gcagtggccg aacgggcaaa aggacgacat ggactgcacg ccggggatcg
     1381 tgcgcgtcga cccgatgctc ggggcgctgg cggacaacgg cggtccgacg cagacggtgt
     1441 cgccgctggc gggcagcccc gcgctcgcgg cgggcagcga gtgcccgacg accgaccagc
     1501 gcggaatgcc gcgcggcgag ccgtgcacga tcggcgcgct cgaggtgcag taagggtggc
     1561 acgatcgcgg gcgggtgcgg atccgacccg gttttgtttc gcgggccagt tgtcccgcgg
     1621 ccgcgggcct gaaatgctct cggcatgtcc gatacacatt cactcgatgc tggcgccgac
     1681 gcgacccccc tgttgatcgt ggattttggt aagcggcaaa agccgaagca gatccgcaag
     1741 ctgcgcaagg gcaccggcaa gctgaccgag aagatccagg agctcgtcga cgagctgcgc
     1801 gagcagcaga cgatcgccgc gggcgcgcag ccggtggtca tcatcgtgcg ggagaagaag
     1861 aagctgatgc cgcgcaagct gttcgggatg tgacaggatg tgacagcgcg taagcgccgg
     1921 acccggcggg cgtcagcggc gtccgcgggg gatcagggcg ccacaggtgg cgcacagcgg
     1981 agggtcgcgg acgatgtcga gggccgggcc gatgccgagc atgtaggcca tcatcagcgg
     2041 cagcgagacc gcgaagtcgc cggcgaggaa ggggagaccc ggcagcaaca tcagcggtat
     2101 ataaaaatag gccttccacc agcgccggac cgcgggcgaa tggtggtact cgacctcgac
     2161 gtcgtgatcg cagcggagac agtgactgcg cgacgcgggg attgctcgta ccacgcggcg
     2221 acggtagcac gccggtatgc ggcgaaccgg gcgtgcgacg cggggccgca gttgacggcg
     2281 ttcaccggcc cgaggccgcg tctccgcgca ggaccatgca ggtgctggcg agccggcaca
     2341 cgagggcgcc ggtgtcgtcg acgatgtcac attcgaggta gccgaggctg cgcgagcgct
     2401 tgaccatctt tgcgtcggcg cgcaggcggg cgttccagac cggcttgaag tagttggcct
     2461 tgagctcgat cgtggtgaag ctctcgccct cgcgcagggt gctggccatg gcgaagccca
     2521 tcgcggcgtc gccgaggtcg acgagcacgc cgccgtgcat ggtgcccatc gggttggcgt
     2581 ggcgggcggg gtcggcttgc agggtgaagc tggcggagcc gctgccgata tcgacgagct
     2641 cgaagccgat cgtgcgggcg atcggtggcg gctcggcgcc catcaggagg tcgcgcgcct
     2701 gcttcatcca cgtggtctcg gtggcgggga tcgtgttgct catgtctgct ccttgggcct
     2761 ggcagtctcg gcgtgctcga gggcggcgag ggcgagggcc cggctggcgg tcaggacggc
     2821 gtcggacagc ggggtgccgg cggtggcgcg cgcgaggctc aggccgccga ccatcagcgc
     2881 gaacaggccg agcacgagct gcttgccgcc gagcggcccg gcgacgcggg cgaagtcggc
     2941 gacgatcgcc tcgatctcct cggcgagggc gtcgcggtag gggccgccga gcgcggcggc
     3001 gtcacccatg gcggccggct gcgggcagcc gcgcgccggg ttgtcgcggt gggcccgcga
     3061 gaggtagcgg cgaacgatcg cggcgtggcg ctcgcgcgtg gtgtcgccgc gcgcgtgctc
     3121 gatcagctga gcccagttgc cgcggaagct gtcgcgcagg gcttcttcaa agagcgcctc
     3181 cttgctgtcc cagtggccgt agaagccgcc gaccgtcagc ttggcgcccg ccatgacctc
     3241 cgcgacggag ggcaaccgca ggccccgcgt gcgcagcaga cgggcggcct cctggcggat
     3301 ccgggtgtgg gtctgggccc accgctcggc cttcgagagc gcctccttca cca
//
LOCUS       DASWNJ010001155         1502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_232215, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001155 DASWNJ010000000
VERSION     DASWNJ010001155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232215"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VGB85_19470"
     CDS             complement(<1..195)
                     /locus_tag="VGB85_19470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0136278.1"
                     /translation="MDDRQRDGEDRFRAMADTAPVLMWMAGPDAQWTFFNASWLRFTG
                     RDMAQELGTGWLDGVHPDDRQ"
     gene            275..862
                     /locus_tag="VGB85_19475"
     CDS             275..862
                     /locus_tag="VGB85_19475"
                     /inference="COORDINATES: protein motif:HMM:NF025268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY0136279.1"
                     /translation="MTPVQRTAAVALVVLLAASFAVAATLTLVVDLSCEGKSPSSQAQ
                     TGAVAPAPPPAPARQATAPAHGYGDKIAWRGLDEGFKEAAALGRPLMLVVHAGWCPRC
                     KELRPRFFDPAIAQASEQFVMVNVDQDETPEALRHGPDGKYIPRILFFDAQGKLDPEL
                     ANPTRSKFKYFYTAHDDLAAMMQRALERHAPAAHL"
     gene            876..>1502
                     /locus_tag="VGB85_19480"
     CDS             876..>1502
                     /locus_tag="VGB85_19480"
                     /inference="COORDINATES: protein motif:HMM:NF017400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HEY0136280.1"
                     /translation="MNATNAMTTVTKQTKSRRRVGLGVLGFGLAALALTTDPVRAAPV
                     DTMPVEDVRPGMKGYAVTVFSGTDSDRFEIEVIDVVRDYLPKQDAILFRSADPRMVHS
                     GIVGGMSGSPIYIEGKLVGALAYGYRFNKDPIGGITPIKNMLTVSDLPFRPEVLPEAR
                     TGPRAQARNGAAGWADAMLGLGVSPLPARRRPADLDPVNGLAPIGAPMA"
BASE COUNT          225 a          559 c          495 g          223 t
ORIGIN      
        1 ctggcggtcg tccgggtgca cgccgtcgag ccagccggtg ccgagctcct gggccatgtc
       61 gcggccggtg aagcgcagcc agctcgcgtt gaagaaggtc cactgcgcgt cgggcccggc
      121 catccacatg agcacgggcg cggtgtcggc catcgcgcgg aacctgtcct ccccgtctcg
      181 ctgccggtcg tccatgggtc ccgcggcatt atccaggccc ggcgaggaca tttccaggca
      241 gaactccgcg agtcgcatgc tatggtcacc ggccgtgact ccggttcaac gcaccgcggc
      301 cgtggcgctg gtcgtcttgc tggcggcatc cttcgcggtg gctgcaaccc tcacgctggt
      361 cgtggacctc tcctgcgagg gtaaatcccc gtccagccag gcgcagaccg gcgccgtcgc
      421 gccggcgcca ccgcccgcac cagcgcgcca ggccaccgcg ccggcccacg gctacggcga
      481 caagatcgcc tggcgcggcc tcgacgaggg tttcaaagag gccgccgcgc tcggccggcc
      541 gctgatgctc gtcgtccatg ccggctggtg tccgcgctgc aaggagctgc ggccgcgctt
      601 tttcgacccg gcgatcgcgc aggcgagcga gcaattcgtc atggtcaacg tcgatcagga
      661 cgagaccccg gaggcgctgc gccacggccc cgacggcaag tacatcccgc ggatcctgtt
      721 cttcgacgcg cagggcaagc tcgaccccga gctcgccaac ccgacccgca gcaagttcaa
      781 gtacttctat accgcgcacg acgatctggc cgccatgatg cagcgagccc tcgaacgcca
      841 tgccccggcc gcccatctgt aacgtgatga acgcgatgaa cgcgacgaac gcgatgacca
      901 cggtgacgaa gcagacgaag tcgcgacggc gggtcggcct cggcgtcctc gggtttgggc
      961 tcgccgcgct cgcgctgacg accgaccccg tgcgcgccgc cccggtcgac acgatgcccg
     1021 tcgaggacgt gcgccccggc atgaagggtt acgcggtcac cgtgttctcg ggcaccgaca
     1081 gcgaccgctt cgagatcgag gtcatcgacg tcgtgcgcga ctatctcccg aagcaggacg
     1141 cgatcctgtt tcgatccgct gacccgcgaa tggtgcactc cgggatcgtc ggcggcatgt
     1201 cgggcagccc gatctacatc gagggcaagc tggtcggcgc gctcgcctac ggctaccgtt
     1261 ttaataaaga cccgatcggc ggcatcacgc cgatcaagaa catgctcacc gtctcggacc
     1321 tgccgtttcg gcccgaggtc ctgcccgagg cgcgcaccgg tccgcgcgcg caggcccgca
     1381 acggcgcggc cggctgggcc gacgcgatgc tcggcctcgg cgtctcgccg ctgccggccc
     1441 gccggcgccc ggctgacctc gaccccgtca acggcctcgc gccgatcggc gcgccgatgg
     1501 cc
//
LOCUS       DASWNJ010001156         5436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_304554, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001156 DASWNJ010000000
VERSION     DASWNJ010001156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304554"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..917
                     /locus_tag="VGB85_19485"
     CDS             177..917
                     /locus_tag="VGB85_19485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136281.1"
                     /translation="MRLRFHRSPGKSTVFFMLFMPFMPVVLVAACGPGTDDSGTGSAT
                     TTTEPGTGDPPTTTTPPTTGEESSGSTTDAPPAAGECAQDSDCILINDCCTCDAAPAD
                     APIKPCEGNCLQSSCDAEGLRDVRVACRLGVCEFAEVDCSEGPVACDETMPSCPEGTR
                     VSVQDGCWGPCVHPRYCLQGEPCPSGGCGAGWTCVQREAGGSVCEVVPLECAGTLGCV
                     CAAPYLDELCPGECSDDSKGAVTCLGGG"
     gene            963..1787
                     /locus_tag="VGB85_19490"
     CDS             963..1787
                     /locus_tag="VGB85_19490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02452 family protein"
                     /protein_id="HEY0136282.1"
                     /translation="MRDPRDIRRSVAAAQGAEAVAIAQAGCYTAPSGRVVDVQEAIAA
                     AVAGTRDLPPDQAVMHAPTRAHATRIEVCNESTLAAARRLVGADPVALNFASAYNPGG
                     GFLGGARAQEESLCRASALHACIVGRPMYAHHSAHRDPMYTAWLMYSPGVPVFRDDDG
                     MLLETPWTCGFITAAAPNVKVLRERSPERLDALPAVMTERITRVLGVAAREGHASVVL
                     GAWGCGAFGGDTGLVAECFHAALHGPFHGVFATVVFAVLDSTAERRFIGPFERMFV"
     gene            1801..2262
                     /locus_tag="VGB85_19495"
     CDS             1801..2262
                     /locus_tag="VGB85_19495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136283.1"
                     /translation="MFIDAITAARIDRAEAELSTAIAIAVGARARERAGVGPRRRRGG
                     VRATRLADQQDDRGRLRGSQSQHNVMRQGFALAYVRAVLVRDAPAAGCQDIGRLPTWS
                     NDPVDLPRAGTHRPPGRIIKILPCGHRILYKDARGWEQRIYRSHGRVENAV"
     gene            2300..4651
                     /locus_tag="VGB85_19500"
     CDS             2300..4651
                     /locus_tag="VGB85_19500"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0136284.1"
                     /translation="MLSTLQMVREPFTAYRRWFAEYGDPVAVPTLHGRVVLTADPEGV
                     KAIFTADADDIDPFLPDVFRPFLGVSSVLLSSGTQHRRQRKLLGPTFHSGRLAAQVPR
                     MVAVCEAVVSRLQPGARVSLRALMQDVTLRVIAEAILCAGEPARADGIARAAYDLIAD
                     FHPILLFAPALHRELGGLGPFARFNRAARVFDEQLLGEIGRRRAAPGDDILGAMLTAR
                     DDQGAGLDDLSIAHQMRTLLFAGYETTGLTLAWALYWLGVHPELRARVLAEVEAVGDP
                     VAATESCPLLAAFCDEVLRVSPILPEVRRTLRAPLLLRGYELPAGTHVSANMAMAHMR
                     PETFSEPERFLPERFMNRRYSPFEFFPWGRRGSPLPRGRLRHARAAGRAGDRAAAISP
                     ALVAARSGPDRPPLGHHVAALRSAGRIRGAPLMPGLRLWLGREQRAFLAAAMQQVSRS
                     FALMTPCLEAPLDSVVATAYLVCRLADCVEDCEAPLAWKRERFAELRAVLATPSAADR
                     LLAAWVDADWRDLAAAEAGLARLGGSSALWSIYAAFPGPVRAAFERWIGVMIEGMART
                     LDPEVSPRWVERGAVHALATADDYDRYCDYVAGTVGQLLTDLATLHYELEPEVVAVLR
                     RHADAAGRGLQKTNILKDYARDLERGVCYLPADWTDTVADAPLGLRGASAAFTEFVVG
                     DVLVDLDALAVYLHAVPASAVGLRLGLLVCLLPAQATMQRATQRPAALFTPEHDIKIS
                     RDTFKRCLLDARHLAGDAAAAVSTIAAGRAAITAALASGSHVP"
     gene            4662..>5436
                     /locus_tag="VGB85_19505"
     CDS             4662..>5436
                     /locus_tag="VGB85_19505"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEY0136285.1"
                     /translation="MERTAGPEKIAVLGGGIASLSAVYELTCLPDWRERFAITVYQMG
                     WRLGGKGASGRNAAAGDRIEEHGLHILMGFYENTHRLLRRCYAALDRPDGAPFARWED
                     AFKPHDFVLHQERFNDEWIDWPVEQEILPGEPGDGGPIPTTRDYLRRLLEYVDTQYDG
                     SPVAELGDDAAPAAALTGAALARLAAVTGLTAAPSPRAGLALARRLLTDGDDEHALVV
                     ALLDRFRGWFGAALGPLRSSHTKAFQAWIAADYALTIARG"
BASE COUNT          701 a         1895 c         1972 g          868 t
ORIGIN      
        1 gtcgtggtgc ccccggtgtc ggggtcgccc gggctgcgag cagtgcacgc ggtggcggcc
       61 gcggtgagga ggagcaggcg cagcgcgaga cgcatgcctc gagtgtccca cgataaaccg
      121 cgactgtcgc ggctccctcg tgatcatcgc ggcgtgcgac ggagtacact gcggcgatgc
      181 gcttgcgatt tcatcgatcc cccggcaaaa gcacggtctt cttcatgttg ttcatgccgt
      241 tcatgccggt cgtgctcgtc gccgcctgcg gccccgggac cgacgacagc gggacgggga
      301 gcgcgacgac gacgaccgag ccgggcaccg gtgacccgcc gaccacgacg acgccaccaa
      361 cgactggaga ggagagcagc gggtcgacca cggacgctcc gccggccgcg ggtgaatgcg
      421 cgcaggacag cgactgcatt ctgataaatg attgctgcac ctgcgacgcg gcgccggccg
      481 acgcccccat caaaccctgc gagggcaatt gcctgcaatc ttcctgcgac gcggagggcc
      541 tgcgcgacgt acgggtggcc tgtcgcctcg gcgtgtgcga gttcgcggag gtggattgca
      601 gcgagggacc ggtggcgtgc gacgagacga tgccgagctg ccccgagggc acgcgggtgt
      661 cggtccagga cggctgctgg ggcccgtgcg tgcacccgcg ctactgcctg cagggggagc
      721 cgtgcccgtc ggggggctgc ggcgcgggct ggacctgcgt gcagcgcgag gccggcggct
      781 ccgtgtgcga ggtcgtgccg ctcgagtgcg ccggcacgct cggttgcgtg tgcgctgcgc
      841 cgtacctcga cgagctgtgc ccgggcgagt gcagcgacga cagcaagggc gcggtcacct
      901 gcctgggcgg cgggtgaata gtggcgctgg acgtcggcgg cgtgtttggc caagctcggg
      961 cgatgcgaga tcctcgtgac attcgccggt ccgtcgccgc ggcccagggt gccgaggcgg
     1021 tggcgatcgc ccaggccggc tgttacacgg cgccgtccgg ccgcgtcgtc gacgttcaag
     1081 aggcgatcgc ggcggcggtc gccgggaccc gcgacctccc gccggatcag gccgtaatgc
     1141 acgcgccgac gcgggcccac gccacgcgca tcgaggtctg taacgagagc acgctggcgg
     1201 ccgcgcggcg gctggtcggg gccgacccgg tcgcgctcaa cttcgcctcc gcgtacaacc
     1261 ccggcggcgg cttcctcggc ggcgcgcggg cccaggagga gtcgctgtgc cgcgcctcgg
     1321 ccctgcacgc gtgcatcgtc ggccgaccga tgtacgcgca tcacagcgcc caccgcgacc
     1381 cgatgtacac cgcgtggctg atgtactcgc ccggcgtgcc ggtgttccgc gacgacgatg
     1441 ggatgctgct cgagacgccg tggacctgcg gctttatcac cgccgctgcg cccaacgtga
     1501 aggtgttgcg tgagcgcagc ccggagcgcc tcgacgcgct accggcggtg atgaccgagc
     1561 gtatcacacg ggtcctcggc gtcgccgcgc gcgagggcca cgcaagcgtg gtgctcggcg
     1621 cgtggggctg cggggcgttc ggcggcgaca ccgggctcgt cgccgaatgc ttccacgcgg
     1681 ccctgcacgg gccgtttcac ggcgtatttg cgacggtcgt gttcgccgtg ctcgacagca
     1741 ccgccgagcg ccgcttcatc gggcccttcg agcgcatgtt tgtttgaacc ggagcttgcg
     1801 atgttcatcg acgcgatcac tgccgcccgc atcgaccggg ccgaggccga gctctcgacc
     1861 gcgatcgcga tcgccgtggg cgcgcgagcc cgggagcgcg caggtgtggg acctcgacgg
     1921 cggcgcggcg gtgttcgtgc gacccggctc gccgatcaac aagatgatcg gggtcggctt
     1981 cgcggctcgc agtcgcagca caacgtgatg cgccagggct tcgcgctcgc gtacgtgcgc
     2041 gcggtgctcg tgcgcgacgc tccggctgcc gggtgccagg acatcggccg gctcccgacg
     2101 tggtcgaacg accccgttga tcttccgaga gcgggcacac atcggccgcc gggccgcatc
     2161 attaaaatcc tcccatgtgg acatcgaatc ctttacaagg atgcccgtgg ctgggaacaa
     2221 cgcatatacc gatcccacgg aagggtcgaa aacgccgttt gaacggccta ttggccgatt
     2281 gccgggcccg cgctcgcgaa tgctctcgac cctgcagatg gtacgagagc cattcactgc
     2341 atatcgtcgg tggttcgcag agtatggcga tcccgtcgcg gtgccgaccc tgcacgggcg
     2401 cgtggtattg actgcggacc cggagggcgt gaaggcgatc ttcaccgccg acgccgacga
     2461 tatcgacccg ttcttgccgg acgtgtttcg cccgttcctc ggcgtcagct cggtcctgct
     2521 atcgtcgggg acgcaacatc gccgccagcg caagctgctg gggccgacct tccactcggg
     2581 tcgcctggcg gcccaggtcc cgcggatggt cgccgtctgc gaggccgtcg tgtcgcggct
     2641 gcagccgggc gcgcgggtgt cgctgcgggc gctgatgcag gacgtgacgc tgcgggtgat
     2701 cgccgaggcg atcctgtgtg ctggcgagcc ggcgcgcgcc gatggcatcg cccgcgcggc
     2761 ctatgatctg atcgccgact ttcacccgat cctgctgttc gcgcccgcgc tgcaccgcga
     2821 gctcggcggc ctcggcccgt tcgcccgctt caaccgcgcg gcgcgggtgt tcgacgagca
     2881 gctgctcggc gagatcggcc ggcggcgcgc cgcgcccggc gacgacatcc tcggcgccat
     2941 gctgacggcc cgggacgatc agggggccgg cctcgacgac ctgtcgatcg cccaccagat
     3001 gcgcaccctg ttgttcgcgg gctacgagac gaccggcctg acgctggcgt gggcgctgta
     3061 ctggctcggc gtgcaccccg agctgcgcgc gcgggtgctc gccgaagtgg aggccgtcgg
     3121 cgacccggtc gccgcgaccg agtcctgccc gctgctcgcc gccttctgcg acgaggtcct
     3181 gcgcgtctcg ccgatcttgc cggaggtgcg acggaccctg cgcgcgccgc tgctgctccg
     3241 cgggtatgag ctgcccgcgg gcacccacgt atcggcgaat atggcgatgg cacatatgcg
     3301 gcccgagacc ttctccgagc ccgagcgctt cctccccgaa cgcttcatga accgccgcta
     3361 cagccccttc gagttctttc cctgggggcg gcggggctca ccgctgcctc gcggccggct
     3421 tcgccacgct cgagctgcgg gccgtgctgg cgaccgtgct gctgcgattt cgcctgcgct
     3481 cgttgcagcg cggtccggtc cagatcgtcc gccactcggc caccatgtgg ccgcgctgcg
     3541 gtctgccggc cgtatacgag gggcgccgct gatgcccggg ctgcgtctgt ggctcggtcg
     3601 cgagcagcgt gcattcctgg ccgcggcgat gcagcaggtg tcgcgctcgt tcgcgttgat
     3661 gacgccgtgc ctcgaggcgc cgctggattc ggtggtggcg acggcgtatc tcgtgtgccg
     3721 gctcgcggat tgtgtggaag attgcgaggc tccgctggcg tggaagcgcg agcgcttcgc
     3781 cgagctgcgc gccgtgctgg cgacgccgtc cgcggccgat cgcctgctcg cagcgtgggt
     3841 cgacgccgac tggcgcgacc tcgccgcggc cgaggccggg ctcgcgcgtc tcggcggctc
     3901 ctcggcgttg tggtcgatct acgccgcgtt cccggggccc gtgcgtgccg ccttcgagcg
     3961 ctggatcggg gtcatgatcg agggcatggc ccgcaccctc gacccggagg tctcgccgcg
     4021 atgggtcgag cgcggggccg tgcatgcgct cgccaccgcc gatgactatg atcgctattg
     4081 cgattatgtc gccggcaccg tcggccaatt gctgacggac ctcgccacgc tgcattatga
     4141 gctcgagccc gaggtcgtcg cggtgctgcg gcggcacgcg gacgcggccg gtcgcgggct
     4201 gcagaagacc aacattctca aggactacgc ccgcgacctg gagcgcggcg tctgctacct
     4261 gcccgcggat tggacggata cggtcgccga cgcgcctctc gggttgcgcg gtgcgtccgc
     4321 ggcgttcacc gagttcgtcg tcggcgacgt gctggtcgac ctcgacgcgc tggcggtcta
     4381 tctccacgcc gtgccggcct cggcggtcgg tctgcggctc ggtctgctgg tctgcctgtt
     4441 gccggcgcag gcgacgatgc agcgcgcgac gcagcggccg gcggccctgt tcacgccgga
     4501 gcacgacatc aagatctccc gcgacacctt caagcgctgc ctgctcgacg cccgccacct
     4561 cgctggcgac gccgcggcgg cggtgagcac gatcgccgcc ggtcgggcgg cgatcacggc
     4621 cgcgctggcg agtggatcac acgtaccata aggagctctg gatggaacga acggctggac
     4681 ccgagaagat cgcggtgctc ggcgggggca tcgcctcgct gagcgccgtc tatgagctga
     4741 cttgcctgcc cgactggcgc gagcgcttcg ccatcaccgt ctatcagatg ggttggcggc
     4801 tcggcggaaa gggggcgagc gggcgcaacg ccgcggccgg cgaccgcatc gaggagcacg
     4861 ggctgcacat cctgatgggc ttttacgaga acacccaccg cctgctcagg cgctgttacg
     4921 cggcgctcga tcggccggac ggggccccct tcgcgcgctg ggaggacgcg ttcaagcccc
     4981 acgacttcgt actccaccag gagcgcttca acgacgagtg gatcgactgg cccgtcgagc
     5041 aggagatctt gcccggcgag cctggcgacg gcgggccgat cccgacgaca cgagattacc
     5101 tgcgccggct cctcgaatat gtcgatacgc aatatgatgg gtcgccagtc gccgagctcg
     5161 gcgacgacgc ggccccggcc gcggcgctga cgggcgccgc gctggcccgg ctggcggcgg
     5221 tgacgggtct gaccgcggct ccttcgcctc gcgcggggct cgccctcgcg cgccggctgc
     5281 tcacggacgg cgacgacgag catgccctgg tcgtcgcgct gctcgatcga ttccgcgggt
     5341 ggttcggcgc ggcgctcggt cccctgcggt ccagccatac caaggcgttc caggcctgga
     5401 tcgccgccga ctatgcgctg acgatcgcgc gcggca
//
LOCUS       DASWNJ010001157         4054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_657431, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001157 DASWNJ010000000
VERSION     DASWNJ010001157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657431"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1706)
                     /locus_tag="VGB85_19510"
     CDS             complement(114..1706)
                     /locus_tag="VGB85_19510"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0136286.1"
                     /translation="MDVLWTPPKAKADPNIRTAYMSDASVENATGQVLCKRFELLAPI
                     GFGATAAVYRALDRDTKSEVAVKILYKSLRDDAEAVRYFAQEGKLAARIVHPHLLRAH
                     YYGQRRGAPFIVFELVPGTSLSQVAAMRPLPWRRLAVIVLQVLDALAKLHEEGVIHGD
                     IQPENVIVQQTTLGQDFAKVIDLGFASARGCSRLTQAAEPPAEVHGTPGFIAPERLAG
                     LDPDARADLYSVGALMYFLLTTRPVPDISLAPEELGVPSPSVMAPGARIPHAIDVIVM
                     RALSDVDDRYPTAADMAHALREALTQPDLPAATPFIVPEATLAAPAGPVIPIAAIEGT
                     EDPPAAITAAAVAGRASAAEPPGSIGVMTQQAATRAPVAVGVGAGLALCGIVVAALWN
                     VGAAQSGGHLNADQAMNLASTPDAGAAAIRRPEPAPAPPNLLPPAPPEPELKPVVPKG
                     SSARRRADDDPRVAEVRGAINRCKPLLPYSRSTITVKPDPRGKTLILFGQHEARGQLG
                     LCVAAIAARTKIAKGDQFSFKL"
     gene            complement(1709..2623)
                     /locus_tag="VGB85_19515"
     CDS             complement(1709..2623)
                     /locus_tag="VGB85_19515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136287.1"
                     /translation="MNGPPLVLCRVLAVSAFLVAGASSARALAGPEQLSFREQAICLK
                     HREANEHALAAEHCLAAYNALPDLTDAMEARSVMAFDAHYSFRDAYVQTGEVKHLCDE
                     IRLFIRFLNYLDQHYPAPERPADRKDAQELLDAARQDLGGRSCKEKLPEPEPVPESKP
                     EPEPEQITAPAPAPAVEVTPPATLKMRGGLRASGWTLFALGLGFGAWSAAELAIGELN
                     QRSRDTLIGSVPAPVPDDLLRQAERLVAAGEAANRRAIVAGSLSGASLLTGITLLAVD
                     VHRRRQHRRLAFAPILGATLGVRLRLEF"
     gene            2717..>4054
                     /locus_tag="VGB85_19520"
     CDS             2717..>4054
                     /locus_tag="VGB85_19520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136288.1"
                     /translation="MTDAVAQRRKKTYGGIGFLALTALPGCFQRTPYPDCWVYSPECV
                     YESAEDGGSGDGTSSMPTSEPFPAGSTETGGDDAGASSGDAGASSGESDVGTTGPDMQ
                     AQPTIVGMSLTPGPGTPQSCVLKSAGPVTVTVQVEDAEEVWITVDGGEAVALEPLGDN
                     ELTFAGEIAVLGESWNGSHAVSAIAQSGELVSAPLGDTFTVEAPAAGSEAWKKKSTIT
                     PSKGNAVAVDAQGEVYELFTQWTQQGTRCHIRRRDTEGEPAWPQDAVALAAGVSCAGE
                     DIEVALDGTIWALTNTVVDEVNRWQLFHLDSDGMLLDAPKVGDFQEFGRGLDMNAAGD
                     LLLCGTRPGLLLPDAWVRLSLSAGKGWTVPWVYKLEGADQEFDERTRDCAFVEDRIVV
                     VGEVFGKHDELDPWKLSRGSVVEFGTNGAKLSEAVAADEPTLQSGYEAVAPDGD"
BASE COUNT          663 a         1251 c         1518 g          622 t
ORIGIN      
        1 cgtgtcacac gcgagcgcga gaatggtgaa aaggcaaaag cagtggctag gccgtcgcat
       61 ggtcggcatg aaaacctcga tggatgtggg gggtgggggg agcgactaac ggattaaagt
      121 ttgaagctga attgatcgcc cttggcgatc ttggtccggg cggcgatcgc agcgacgcac
      181 aggccaagct ggcccctcgc ctcgtgctgg ccgaacagga tcaaggtctt gccgcgcggg
      241 tcgggcttga cggtgatcgt cgatcttgag tacggcaaca gcggcttgca gcggttgatc
      301 gcgccgcgta cctccgcgac acgcgggtca tcgtcggcgc gtcgcctcgc gctgctgccc
      361 ttcggaacca cgggcttcaa ttccggctca gggggcgcgg gggggagcag gtttggcggg
      421 gctggggccg gctcgggtcg gcggatcgca gcagctccgg cgtcgggtgt agaggccaag
      481 ttcatcgcct ggtcggcgtt caagtgcccg ccgctttgcg cggcaccgac gttccacagc
      541 gccgcgacga cgatcccaca caaggccagt ccggcgccca cgccgacggc aaccggcgcg
      601 cgggtggcag cctgctgcgt catgacgccg atggatccag gcggctcggc cgcggacgcg
      661 cggccggcga ccgcggcggc cgtgatcgcg gccggcggat cctcggtccc ttcaatcgcg
      721 gcgatcggga tgaccggccc cgcaggcgcg gccagcgtcg cctcggggac gatgaatggg
      781 gtggcggcgg ggaggtccgg ctgcgtgagc gcttcgcgga gcgcatgtgc catgtccgcc
      841 gcggtcgggt atcgatcgtc cacgtcgctg agcgccctca tcacgatcac atcgatcgcg
      901 tgggggatgc gcgcaccggg ggccatgacg ctcggcgacg gcacgcccag ctcctcgggc
      961 gcgaggctga tgtccgggac cgggcgtgtc gtcagcaaga aatacatgag cgccccgacc
     1021 gagtagaggt cggcgcgggc gtcaggatcg aggccggcga ggcgctcggg ggcgatgaag
     1081 ccgggcgtgc catgcacctc ggccggcggc tccgcggcct gcgtgagtcg gctacagccg
     1141 cgggcgctgg cgaagcccag atcgatgacc ttcgcgaaat cctggccgag cgtcgtctgc
     1201 tggacgatca cattttcggg ctgaatgtcg ccgtggatga cgccctcctc gtggagcttg
     1261 gcgagcgcgt cgagcacctg caggacgatt accgcgaggc gccgccacgg cagcggtcgc
     1321 atggccgcga cctggctgag cgacgtgccg gggaccagct cgaagacgat gaagggcgcg
     1381 ccgcggcgct ggccgtagta gtgcgcgcgc agcaggtgcg ggtggacgat gcgggccgcc
     1441 agcttgccct cctgcgcgaa gtagcggacg gcttcggcgt cgtcgcgcag gctcttgtag
     1501 aggatcttga ccgcgacctc actcttggta tcgcggtcga gcgcccggta cacggcggcg
     1561 gtcgccccga agccgatcgg cgcgagaagc tcgaagcgct tacacaacac ctggcccgtc
     1621 gcgttctcga cgctcgcgtc cgacatatac gcggtgcgta tgttagggtc ggccttggcc
     1681 ttgggcgggg tccacaggac gtccatgatc aaaactccag gcgcaggcgg acgccgaggg
     1741 tcgccccgag gatgggtgcg aacgcgaggc ggcgatgttg ccggcgacga tgaacgtcga
     1801 ccgcgagcag cgtgatgccg gtgagcaacg aggcgccgga gagactgccc gccacgatgg
     1861 cccgtcggtt ggcggcctcg cccgctgcga ccaacctctc ggcctggcgc agcagatcgt
     1921 ccggcaccgg cgcgggcaca gagccgatca acgtgtcgcg gctgcgctgg tttagctccc
     1981 cgatcgccag ctccgcggcg ctccacgccc cgaacccgag gccgagtgcg aacaacgtcc
     2041 acccggaagc acgtagaccg ccacgcatct ttaacgtcgc gggcggcgtg acctcgacgg
     2101 ccggcgccgg agctggtgca gtgatctgct cgggttcggg ttcgggtttg gactccggta
     2161 ctggctccgg ctcgggcaat ttctctttgc agctacggcc gccgaggtct tgacgggcag
     2221 cgtccaggag ttcctgagcg tccttgcgat cggcgggccg ctccggtgcg gggtagtgct
     2281 gatcgaggta gttgaggaat cgaatgaaga ggcggatctc gtcgcagagg tgcttgacct
     2341 cgccggtctg gacgtaggcg tcgcggaacg agtagtgcgc gtcgaaggcc atcacggacc
     2401 tggcctccat ggcatcagtc aggtcaggaa gggcgttgta cgcggcgagg cagtgctcgg
     2461 ccgccagggc gtgctcgttc gcctcgcggt gcttgaggca gatagcttgc tcgcgaaatg
     2521 agagctgctc cggaccggcg agcgcacgcg ccgacgaggc cccggcgacg aggaaggcgg
     2581 agacggccag gacacggcag agaacgaggg ggggcccgtt cataggcgtc caagctaccg
     2641 gcactcgccc acgttgccaa gatgacgcac cgacaattcg cgtgacagcg ggttagcagg
     2701 ggggtagcct ggcggaatga ccgacgcggt tgcccaaagg cgtaagaaaa cctacggcgg
     2761 aattggattt ctggcgctaa ccgcgctgcc cgggtgcttt cagcggacgc catacccaga
     2821 ctgctgggtc tactcgcccg agtgcgtgta cgagagcgcc gaggatggcg gctccggcga
     2881 tggaacatcg tccatgccga caagtgagcc gtttccggca ggaagcacgg agaccggcgg
     2941 cgacgatgca ggggccagct cgggcgacgc gggagccagc tcgggcgaga gcgacgtggg
     3001 gacgacgggc ccagacatgc aggcgcagcc gacgatcgtc ggcatgagcc tgaccccggg
     3061 gcccggtacg ccgcagtcgt gcgtactgaa gtcggccggg ccggtgacag tgacggtcca
     3121 ggtcgaggac gccgaggagg tctggatcac agtcgacggc ggcgaggccg tcgcgctcga
     3181 gccgctcggt gacaacgagc tgacgttcgc tggggagatc gcggtgctcg gcgagtcgtg
     3241 gaacgggtcg cacgccgtct cggcgatcgc ccagagcggc gagctggtga gcgcgccact
     3301 cggggacacg ttcaccgtgg aggccccggc agctgggtcg gaggcgtgga agaagaagag
     3361 tacgatcacg ccgagcaagg ggaacgcggt cgccgtcgac gcccagggcg aggtctacga
     3421 gcttttcact cagtggaccc agcaaggaac ccggtgccac atccggcgcc gcgacaccga
     3481 gggtgagccg gcgtggccgc aggatgcggt cgctctcgct gccggggtga gctgcgccgg
     3541 cgaggacatc gaagttgcac tggatggcac gatctgggcg ctgaccaaca ctgttgtcga
     3601 tgaggtcaac cgctggcagc tcttccatct ggactccgac ggcatgttgt tggatgcgcc
     3661 gaaggtcggt gactttcagg aatttgggcg tgggctcgat atgaacgccg cgggcgatct
     3721 gctcctgtgc ggcacgcgac cgggcctttt gttgccagac gcctgggtcc ggctgtcact
     3781 gtcggcaggc aagggctgga cggtgccgtg ggtctacaaa ctcgagggcg ctgaccagga
     3841 gttcgacgag cggacgcggg actgcgcgtt cgtcgaagac cgcatcgtgg tcgtgggcga
     3901 ggtcttcggt aagcacgacg agctcgaccc ctggaagctg agccgcgggt ctgtcgtgga
     3961 gtttggcacc aacggcgcca agctctccga ggccgtggcc gcggatgaac cgaccttgca
     4021 gagcggttac gaggcagtcg ccccggatgg cgac
//
LOCUS       DASWNJ010001158         2843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_403626, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001158 DASWNJ010000000
VERSION     DASWNJ010001158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403626"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="VGB85_19525"
     CDS             <1..1333
                     /locus_tag="VGB85_19525"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0136289.1"
                     /translation="KFQGSVVVRRNGELLYSRNFGRADIASDRPITDHTPFKIGSLTQ
                     QFTAVAVLQLREQGKLQLDDPVRKHLPWYPQATITIRHLLTHSSGIPNYTDSLEAAGV
                     KTGTRYKPQDIAAIFKDAALEFPPGSEFDPSNSGYFLLGMVIEQLAGVPLGEYMQKYV
                     LGPAGMGDSRLGNQVRINGEADGLATGYIFSEDELLVPAVAYDLSVYGGAAGMVSTPT
                     DLSRWDDALRRPGALLSQASLDEMFSPVIDTYGLGWIVLKERGQTMVGHPGGVEGFNG
                     AIARYLGDGITVIALANTEAIDCRDVLDAVAAIAHGEQVPPFTEHEEVPVSPAMFSRY
                     LGNFRLTPTARTRLAAVIDREALGLMDEVEIYDDDGRLFMLVPMHGAKWLHGLGEDRF
                     FFKDQAGTIAQFGPPGAPVEWLRLWQGDLEFVLAKEKETRSDPPGQPSPAR"
     gene            1472..2203
                     /locus_tag="VGB85_19530"
     CDS             1472..2203
                     /locus_tag="VGB85_19530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136290.1"
                     /translation="MKAPLAIVALSLFLGPVAGCDGGEKKSIADQIGPATKASTDVKP
                     TSKVSEEELAKRRKEAGFKTKEEVDAQLAAENAKMFEKGEREFVKARLKDYRKIVADT
                     RKLVDDVEKEAGKWSTAKDPQKAFDKNGEALGKRAKDIQKALDKLTEKGIKGGNTQAT
                     LNKAYRPLEELGGALGPELAKQPAFAETLKTIRAALDEAELAFTDIDKDESLVASKFA
                     KTGEEPAGGDEGAAPADDKAKAGKK"
     gene            2267..>2843
                     /locus_tag="VGB85_19535"
     CDS             2267..>2843
                     /locus_tag="VGB85_19535"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136291.1"
                     /translation="MSATTLVFFILGLVLLVVGADLLVAGASRIAARLGISSLVIGLT
                     VVAFGTSAPELAVSVTGALNDQADLALGNVVGSNIFNALFILGIAALVAPLVVQPQLM
                     RLDVPIMILVSGVCWLMALDGSIGRVEGIALFLGIVVYTTLLIRMARRQGVAAAASVA
                     ANVSIADNVTPHKGLRDRVITQLAMILGGLVM"
BASE COUNT          486 a         1035 c          914 g          408 t
ORIGIN      
        1 caagttccag ggcagcgtcg tcgtgcgccg caacggcgag ctgctgtaca gcaggaactt
       61 cggccgcgcc gacatcgcca gcgaccgccc gatcaccgat cacaccccgt tcaagatcgg
      121 cagcctgacc cagcagttca ccgccgtcgc ggtcctgcag ctgcgcgagc agggcaagtt
      181 gcagctcgac gacccggtgc gcaagcacct gccctggtac ccgcaggcca cgatcacgat
      241 ccgtcacctg ctgacccaca gctcggggat cccgaactac accgatagcc tggaggccgc
      301 cggcgtcaag accggcacgc gttacaagcc gcaggacatc gccgcgatct tcaaggacgc
      361 cgcgctcgag ttcccgcccg gcagcgagtt cgaccccagc aactccggct acttcctgct
      421 cggcatggtc atcgagcagc tcgccggcgt gccgctgggc gagtacatgc aaaagtacgt
      481 gctcggcccc gccggcatgg gcgacagccg actcggcaac caggtccgca tcaacggcga
      541 ggccgacggc ctcgccaccg gctacatctt cagcgaggac gagctgctgg tccccgccgt
      601 cgcgtacgac ctctcggtct acggcggcgc ggccggcatg gtctcgacgc cgaccgacct
      661 gtcgcgctgg gacgacgcgc tacgcaggcc cggcgcgctg ctctcgcagg cctcgctcga
      721 cgagatgttc agcccggtga tcgacaccta cggcctcggc tggatcgtcc tcaaggagcg
      781 cggccagacc atggtcggcc accctggcgg cgtcgagggc ttcaacggcg cgatcgcccg
      841 ttacctcggc gacggcatca ccgtgatcgc cctcgccaac accgaggcga tcgactgccg
      901 cgacgtgctc gacgccgtcg ccgcgatcgc ccacggcgag caggtcccgc cgttcaccga
      961 gcacgaggag gtacctgtct ctccggccat gttctcgcgc tacctcggca acttccgcct
     1021 gaccccgacc gcgcgcacgc ggctcgccgc cgtgatcgac cgcgaggccc tcggcctgat
     1081 ggacgaggtc gagatctacg acgacgacgg ccgcctgttc atgctggtgc cgatgcacgg
     1141 cgcgaagtgg cttcatggcc tcggcgagga ccgcttcttt tttaaagacc aggccgggac
     1201 gatcgcccag ttcggcccgc ccggcgcccc cgtcgagtgg ctgcgactgt ggcaaggcga
     1261 cctcgagttc gtgctcgcca aagaaaagga gacccgcagc gacccgccgg gccagccctc
     1321 gccggcccga tgatgaggat cacgaggatt ctgatcctga tgtggatctc gatgacgtcg
     1381 gacccggatg acgtaggtcg cgatttcccg cgggcgattt gacacgctct ccgcggcccg
     1441 cgtatattgc gccgctgaag agagccctgt catgaaagca cccctcgcca tcgtcgccct
     1501 gagcctgttt ctcggacctg tcgccggctg tgacggcggc gagaagaagt cgatcgccga
     1561 ccagatcggg ccggcgacca aagcctcgac cgacgtcaag cccacctcca aggtctccga
     1621 ggaggagctg gccaagcgcc gcaaggaggc cggcttcaag accaaggagg aggtcgacgc
     1681 ccagctcgcc gccgagaacg ccaagatgtt cgaaaagggc gagcgcgagt tcgtcaaggc
     1741 gcgcctcaag gactaccgca agatcgtcgc cgacacccgc aagctcgtcg acgacgtcga
     1801 gaaggaggcc ggcaagtggt cgaccgccaa ggacccgcaa aaggccttcg acaagaacgg
     1861 tgaggcgctc ggcaagcgcg ccaaggacat ccagaaggcg ctcgacaagc tgaccgagaa
     1921 gggcatcaag ggcggcaaca cccaggcgac cctcaacaag gcctaccgcc cgctcgagga
     1981 gctcggcggc gcgctcgggc ccgagctcgc caagcagccc gccttcgccg agaccctcaa
     2041 gacgatccgc gccgcgctcg acgaggccga gctcgccttc accgacatcg acaaggacga
     2101 gagcctggtc gccagcaagt tcgccaagac cggcgaggag cctgccggcg gcgacgaagg
     2161 cgcagctccg gccgacgaca aggccaaggc cggcaagaag tagccgcgcc gccgcgcgct
     2221 gacgcgcacg ggcgcggcgc ccggcggggt atacaatcgc ggcgtcatgt ccgcgaccac
     2281 cctcgtattc ttcatcctcg ggttggtgtt gctggtcgtc ggcgccgacc tgctggtcgc
     2341 gggcgcctca cggatcgcgg cgcgcctggg gatctcgtcg ctggtcatcg gcctgacggt
     2401 cgtcgccttc ggcacctcgg cgcccgagct cgcggtgtcg gtcacgggcg cgctcaacga
     2461 ccaggccgac ctcgcgctcg gcaacgtcgt cggcagcaac atcttcaacg ccctgttcat
     2521 cctcggcatc gccgcgctgg tcgctccgct ggtggtccag ccgcagctga tgcgcctcga
     2581 cgtgccgatc atgatcctcg tctccggcgt gtgctggctg atggcgctcg acggatcgat
     2641 cggccgcgtc gagggcatcg cgctgttcct cggcatcgtc gtctacacga ccctgctgat
     2701 ccgcatggcg cggcggcagg gcgtcgccgc ggccgcctcg gtcgccgcca acgtctcgat
     2761 cgccgacaac gtcaccccgc acaagggcct gcgcgaccgc gtgatcacgc agctcgcgat
     2821 gatcctcggc ggcctggtga tgc
//
LOCUS       DASWNJ010001159         2015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_21755, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001159 DASWNJ010000000
VERSION     DASWNJ010001159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21755"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VGB85_19540"
     CDS             complement(<1..472)
                     /locus_tag="VGB85_19540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136292.1"
                     /translation="MKSSRLFRSLTNLALIAAMMPLGGCIINVLPEIESDTGSTGEPG
                     TSGDDPTTGASATTATTDTPTTGGPGDDGGAPTTGEPGDPTGAGGECDFAGEIQPIFT
                     ASCAGCHGGGQPAQSLSLAEGAAYAALVEVDSVGMPGTLRVAPGDPASSFLVHKL"
     gene            607..1485
                     /locus_tag="VGB85_19545"
     CDS             607..1485
                     /locus_tag="VGB85_19545"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0136293.1"
                     /translation="MEPGWDRVVDYYERAGPDYRAWSPRFNMHFGYWRRGMNPFRLEP
                     MLAAMNDEILRRLEIPAGTAATVLDLGCGMGETACQLAGSHAGLRIVGLTKVASHVIA
                     GRRKIAAAGLGERVQILHADYTAIPLPDGSVDHAYAIESSCHAQGVDKAAFVRELGRV
                     LRPGGRFVIADGFMKHARPLPAWLARLRARVCEGWAIETFAELPAITERLRACGMQAL
                     EVRDISWRVAPSVVHVPRVALCFLLGQLRSRGSLELDAARRGNVLAPVLGMLLGLARA
                     HFGYHLIAGQRSDPGP"
     gene            1681..>2015
                     /locus_tag="VGB85_19550"
     CDS             1681..>2015
                     /locus_tag="VGB85_19550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136294.1"
                     /translation="MTTHDPRWSPRPLLALFAALLAGCTGREVPEQSIEETEGASTSS
                     SSPGDPATASTTDTSGTDTGTGSTSVPGTVSDPTYEPPECVYADHLIVLTPEEYEAWL
                     HGDATTGDTT"
BASE COUNT          273 a          745 c          699 g          298 t
ORIGIN      
        1 cgagcttgtg gacgaggaag ctgctcgcgg gatccccggg cgcgacgcgc agcgtgccgg
       61 gcatcccgac gctgtcgacc tcgacgagag ccgcatacgc ggcgccctcg gcgaggctga
      121 ggctctgggc cggctggcca ccgccgtgac acccggcgca gctggccgtg aaaatcggct
      181 ggatctcgcc cgcgaagtca cattcaccgc cagcgccggt cggatcaccg ggctcgcctg
      241 tcgtcggcgc gccgccgtcg tctccgggcc cgcccgtcgt cggcgtgtcc gtggtcgcgg
      301 tcgtcgcaga tgcaccggtc gtcgggtcgt caccggaggt gccgggctcc ccggtggacc
      361 cggtgtcgct ctcgatctcc gggagaacat taataatgca gccacccagc ggcatcatgg
      421 ccgcgatgag cgcgagattg gtcagcgaac ggaacaggcg cgaggacttc attgcaaccc
      481 tcagtacccg cgccgaggcg tctgttacac ggttctgcga agtttgtggc cacacgcccc
      541 cgcacctggc gggcggtccg cccgcagcta tgggcgcggc gcccgcccac gctacactgc
      601 cgggcgatgg aaccggggtg ggatcgtgtg gtggattact acgagcgtgc ggggccggac
      661 tatcgcgcct ggagcccccg cttcaacatg cattttggct actggcgtcg cggcatgaac
      721 ccgtttcgcc tcgagccgat gctcgcggcg atgaacgacg agatcctccg tcgcctcgag
      781 atccccgccg gcacggcggc gaccgtgctc gacctcggct gcggcatggg tgagacggcg
      841 tgccagctcg cgggctcgca cgccggcctg cggatcgtcg gcctgacgaa ggtcgccagc
      901 cacgtgatcg ccggccgaag aaagatcgcc gccgcgggcc tcggcgagcg cgtgcagatc
      961 cttcacgccg actacaccgc gatcccgctg cccgacggct cggtcgacca cgcctacgcg
     1021 atcgagagca gctgtcacgc gcagggcgtc gacaaggccg cgttcgtgcg tgagctcggc
     1081 cgcgtgctgc gaccgggcgg acgcttcgtc atcgccgacg gcttcatgaa gcacgcgcgc
     1141 ccgctgccag cctggctcgc ccgcctgcgc gcccgcgtgt gcgagggctg ggcgatcgag
     1201 accttcgccg agctgcccgc gatcaccgag cgactgcggg cttgcggtat gcaggcgctc
     1261 gaggtccgcg acatctcctg gcgcgtcgcg ccctcggtcg tgcacgtgcc gcgcgtcgcc
     1321 ctctgctttt tactcggcca gctgcgcagc cgcggcagcc tcgagctcga cgcggcccgc
     1381 cgcggcaacg tgctcgcgcc ggtgctcggc atgctgctcg gcctggcgcg cgcccacttc
     1441 ggctatcacc tgatcgccgg ccagcgcagc gaccctggac cctgatcgcc tcgcgtttat
     1501 atcgtcgcgt gccgatggcc ccggcccgcg aggtgcgcgc gcgcgccggg ttcggcgagg
     1561 tgttcaccgc ccacgtgcgc gcgccgtgtc aggcccctga cagccccgtc agggtcatgg
     1621 cgcgccgggg catgtagcgt catgccgtcg ggttggccgg cgacttgcac tgccggggtc
     1681 atgacgacgc atgacccgcg ctggagtcct cgaccgctgc tggccctgtt cgcggcgctg
     1741 ctcgccggct gcacgggccg cgaggtgccc gagcagtcga tcgaggagac ggagggcgcg
     1801 agcacatcat cgagcagccc gggcgacccg gccacggcca gcaccaccga cacgtcgggc
     1861 acggataccg gcacgggctc gacgagtgtc cccggcaccg tctccgaccc gacctacgag
     1921 ccccccgagt gcgtctacgc cgaccacctc atcgtgctga ccccggagga gtacgaggcc
     1981 tggctgcacg gcgacgccac gaccggcgac accac
//
LOCUS       DASWNJ010001160         8995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_91055, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001160 DASWNJ010000000
VERSION     DASWNJ010001160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 8995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8995
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91055"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1175
                     /locus_tag="VGB85_19555"
     CDS             93..1175
                     /locus_tag="VGB85_19555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY0136295.1"
                     /translation="MTPRPLAPLFAALLASLTQPGCPGECVFYDVYEFELPADALASY
                     LADDTLTMEECEEACRIPHLPQMTGEATSSSSGSSEPTTGGTTSGGASGTGGTDGTDG
                     SSGGSDTSSYMDTEFHDWGPLVKCSTRTVDATTVALTCKYRDPLTACGRCPIGTVHGR
                     REPVADPTLAWLLAAAELEAISVPAFTELAADLARHGAPDELRQAALDAADDERRHTA
                     AIVALARRRGGAPVPPSVDPAPAADLETLASHNAVEGCVRETWGALVAAFQARNAAEP
                     DVRAAQARIAEDERTHAELAWRIDAWVRPRLSPSARARVDAARSAAVRILLAAVEQLG
                     IGQADVGLPNSSQARALVRGLQTALW"
     gene            1491..2843
                     /locus_tag="VGB85_19560"
     CDS             1491..2843
                     /locus_tag="VGB85_19560"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HEY0136296.1"
                     /translation="MTLSSRFISICLVGATACSSSPNSEGSEGSSSSTGSSTGSSTGT
                     TIEDPTGSPDTTTSGVSDGGSDSTTGTTSTSTTEVSSGSTSTDASSSSGTTGADTDGG
                     LPSTTFDESLCTIFVDATAGSDSNSGKSKEMARKTIGAAVDIVAPGDTVCVYPGTYGA
                     FKNTVSGTEDARIHFVSVERWQAKVVDATVPLRNNGDYVDIDGFEVTTTAEAVGVGIA
                     NGDGQLASHCRIFNNHVYGIVAKTEGGSGGAGILSAGWVSKMPYQGTDVEIFNNVVHD
                     IGNDGVNAKFVQGIYISHPHAKIYNNIVYNVSGWGIHGWHNANYVTVSNNLTSNTNGI
                     VMGNGDSPCDEITCEFTDYFVTNNILYDDRVGIQLYDGPNHTIANNDFYLTDPSGDNA
                     MTDDPLLVDYALDISADFHLAAGSPMIDAGSAEFAPALDFDGKARPQGDAPDVGPFEH
                     "
     gene            complement(2926..4764)
                     /locus_tag="VGB85_19565"
     CDS             complement(2926..4764)
                     /locus_tag="VGB85_19565"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY0136297.1"
                     /translation="MIRLPLVLAAALAACAAAPTAASSQTRHPQTPSPRQVDYTLEIP
                     APAQQWVHVTMTVPRPKGTRSQVAMPAWTPGSYLVRDHARHVDGLRAEDLDGKPLPVQ
                     RVDKQTWRVDHGRRGFRVRYRVFADEPGVRTSFVDDRHASLNGPSTFMYLVGQTSRPA
                     AVEVKLPARWQAHSALEPAGAISTTSANFTAPDYDTLVDAPIVLGTPELRTFDVGPAR
                     IELVFSAPAGSNADTARIAADLQKIVGTFARLMGGLPLRRYVFLVLADETSDGGLEHG
                     TSTLLRVQRDMFSEPAGYRRFANLAAHEFFHLWNVKRLRDAALVPYDYSRENYTRLLW
                     FHEGFTETMEQQALVRADIVPPETHVRELGEAWTAYLRKPGRNHTPLGDLSQDAWIKA
                     YKPSPAHGNTLISYYEKGHLLGVCLDLELRLRSAEHGQQGSLLGLFRRLMASHGQTGA
                     GLTQADIVAAASAEAGEPMDAFFSKYVDGTAELPLPELLARIGVEVTSEAASKAYAGL
                     VLTPERTIRNIEPASPAAAAGLMLGDELIAVAGKRVRSSDEALARISERKPGERVEVA
                     VFRGGRLELRTVTVTADPHRSFRFELAADAPPRTLGLRNAWLARTP"
     gene            complement(4795..6285)
                     /locus_tag="VGB85_19570"
     CDS             complement(4795..6285)
                     /locus_tag="VGB85_19570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0136298.1"
                     /translation="MASSLMSNGIPSERDLLSDDALRARALPAGLYPAAGVTATLAMR
                     ALARWSASGLTVDLVEGLAYLGGALVLGAAVLLLHRDRSLAAARLAIAGWLATTIAVG
                     LTAETLAALGLTPAALAVAIGLSTVFDARSVLRWTLPCSALWAAIVLARSQLYPAEIP
                     HTDLRLLLIVVPTAMLLIVGLAARAAVRGRELARSAARTARVALAERQAELMQSNVEL
                     TRRSGETVAAQSAADAAAQASKAKSAFLAHMSHELRTPLNAILGYVELVRDEAGERGM
                     SQIVADAERMETAGRHLLQLIGDILDISKIEAGQLRLDPVRFDLAQCLRELVETMEPA
                     IQRRSNTLLVEVLLGDDRMVVGDPVCTRQIVFNLLANAAKFTEHGEIGLAVHREGPWL
                     VLRVADSGIGMTSEQIGRVFEPFEQADAATARRYGGTGLGLTITLGLLKLMGGVLTVD
                     SAPGVGTVFLVKIPADITAARPTPGSPPAPQLGERSVRFSTLRARA"
     gene            complement(6436..8061)
                     /locus_tag="VGB85_19575"
     CDS             complement(6436..8061)
                     /locus_tag="VGB85_19575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136299.1"
                     /translation="MSPASPVTATPPAVLAEVVAHFGERVVDIQHVGVEPEPRVPAAG
                     WYTLGACAMVLGLGLVMTSLPDAPRIEPTDTEAVDAPAPAPGPGAAGLGALLVLLGVV
                     PLVIAARSQHVPRDRYVIGEGPDVHLPVALPPGATQAGVPLVRALERQIVLGLVPGMT
                     GELHDGAQTTAVADLVAQGRSSYALPADARADLRLGDLRFTVQAVERAAFVPKRGRLD
                     RLYMLSNAGALAALGALLLLGEPSTGELEIEEQSARRLLAVQYLTDVPPPPPPPPAVA
                     PPVPRDRPSSAPPKPAAPPPAPSTVDELAVEVTGPAQIVPRGTRRGIRDDHSYARVAG
                     VLNDAEFNASIQRATADTQEGLLGYDNEADRAMWVAVRDAPVINRPLGGLELAETERG
                     GGLHDDRPKPTKAPGKNVEIDMYAKRTPPSAAAIAAARRVVTIELETPNVVGELTPLT
                     IRESLRKQADGLKRCFRETVDNAERVGHVVLMLKVAGAGKVTSAQLKWGSTQLGPIGP
                     CITRIASTWKFPATLDRMPASIVVEVVYSAKDM"
     gene            8208..>8995
                     /locus_tag="VGB85_19580"
     CDS             8208..>8995
                     /locus_tag="VGB85_19580"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HEY0136300.1"
                     /translation="MFLAPLLLVACNSGGGGETGEATTGSSGSSGSSVPGDSSGTGSS
                     GGTTVTTGSELPTTGESETTGDVSTGGVTVTGSDTDVGTTGGTSDLPGEAGWRSVLYP
                     EDWTPAFTGPEGRFLHDFSYAGYHLGETPLAVDVSANKIDVVADHGADPTGTADATAA
                     FQAALDAAGLAGGVVEVPAGLYKIEGSLAVKSSRVVLRGVGAAQSKLWFTRFEGMSYK
                     SHLTFGGPLELTDETLLTADGEPRTNTVQVADAAAFEAGDDVALG"
BASE COUNT         1315 a         3013 c         3410 g         1257 t
ORIGIN      
        1 ctgtgcgcct gcgcgcgact ggcgacggct tgctcagctg cggcggccct gcccgcgctt
       61 gcgggtgggc ggcccgccgg tatcatggga gaatgacgcc tcgcccgctc gcgccgctgt
      121 tcgccgcgct cctcgcgtcc ctgacccagc ccgggtgccc cggcgagtgc gtcttttacg
      181 acgtctacga gttcgaactt cccgccgatg ccctcgcctc gtacctcgcc gacgatacac
      241 tgaccatgga ggagtgcgag gaagcgtgcc ggatccccca cctcccgcag atgactggtg
      301 aggcgaccag cagttcgtca ggttcgagcg agccgacgac aggcggcact acgtccggcg
      361 gcgccagtgg taccggcggt acggacggca ccgacggatc gagcggcggc tcggatacgt
      421 cgtcctacat ggacaccgag tttcatgact gggggcccct cgtgaagtgc tcgaccagaa
      481 ccgtcgacgc cacgacggtc gccctgacct gcaagtaccg cgaccctctt accgcctgcg
      541 gtcggtgccc gatcggcacc gtgcacggcc gccgcgagcc ggtcgcggat ccaacgcttg
      601 catggctgct cgccgccgcc gagctcgagg ccatctcggt ccctgccttc accgaactgg
      661 cggccgacct cgcgcggcac ggggctcccg acgaacttcg gcaggctgcg ctcgacgccg
      721 ctgacgacga gcgccgccac actgccgcga tcgtggcgct cgctcgacgt cgtggtggtg
      781 caccggtgcc gccgtcggtc gaccccgcgc cggctgccga tctcgagacc ttggcctccc
      841 acaacgccgt cgagggctgc gtgcgtgaga cctggggcgc actggtggcg gcgttccagg
      901 cccgcaacgc cgccgagccc gacgtgcgcg ctgcacaggc gcgcatcgcc gaggacgagc
      961 ggacccacgc cgagctcgcc tggcgtattg acgcctgggt tcggccacgc ctgtccccga
     1021 gcgctcgggc ccgcgtcgac gcggcccgaa gcgcggccgt tcggatcctc ctcgccgccg
     1081 tcgagcagct tggcatcggc caggccgacg tcggcctccc gaattccagc caggctcgcg
     1141 ccctcgtgcg ggggctacaa actgcgctgt ggtgacgacg cttggcagta ccgcggctga
     1201 gccaacgagg ggccgcaagg accattacgg ctcgcgccgc tctgcgcaac cgggaccgcg
     1261 atacctcacg acttcaccgc gagcagcggg agcccgcggc aatgcagggt atcgacggcc
     1321 ccatgcgcgg cgcacgtgcg ggcgagcgac cgcaatggcg aagatcgccg cgcgcgagcg
     1381 caccatcatc gtggtcgacg ggccgcgata cgcgagtagg gatgcggcgc tcgacaccca
     1441 cggagctttc gatttgacag cgatcggagc gacccctagc gtgcgccgcc atgactctct
     1501 ccagccggtt tatctcgatc tgcctcgtcg gcgcgacagc gtgctcctcc tcgccgaaca
     1561 gcgagggtag cgagggcagc agcagcagca ccggcagcag caccggcagc agcaccggca
     1621 ccaccatcga ggacccgacc ggcagcccgg acacgaccac gtcgggcgtc tccgacggcg
     1681 gcagcgacag tacgaccggc acgacgagca cgagcaccac cgaagtcagc agcggcagca
     1741 cgagcaccga cgcgagcagc agcagcggca cgaccggggc ggacacggac ggcgggctgc
     1801 cgtcgacgac cttcgacgag agtctgtgca cgatcttcgt cgatgcgacc gcggggtcgg
     1861 acagcaacag cggcaagtcc aaggagatgg cgcgcaagac gatcggcgcg gcggtcgaca
     1921 tcgtcgcgcc cggtgatacg gtctgcgtgt atcctggcac ctacggcgct ttcaagaata
     1981 cggtcagcgg caccgaggac gcacggatcc actttgtctc ggtcgagcgg tggcaggcga
     2041 aggtggtcga cgcgacggtc ccgctgcgca acaacggcga ctacgtcgac atcgacggct
     2101 tcgaggtgac gacgaccgcc gaggcggtcg gcgtcgggat cgccaacgga gacgggcagc
     2161 tcgcctcgca ttgccggatc ttcaacaatc acgtgtacgg gatcgtggcg aagaccgagg
     2221 gcggcagcgg tggcgccggc atcctgtcgg cgggctgggt gtcgaagatg ccgtatcagg
     2281 gcaccgacgt cgagatcttc aataatgtcg tccacgacat cggcaacgac ggcgtcaacg
     2341 cgaagttcgt ccaggggatc tatatcagcc acccgcacgc gaagatctac aataatattg
     2401 tctataacgt gtcaggctgg ggcatccatg gctggcacaa cgccaactac gtgacagtct
     2461 ccaacaacct gacctcgaac accaacggga tcgtgatggg caacggcgac tctccttgcg
     2521 acgagatcac ctgcgaattc accgactatt tcgtgaccaa caatattttg tacgatgacc
     2581 gcgtcgggat ccagctgtac gacggcccca accacaccat cgccaacaac gacttctatc
     2641 tgaccgatcc gagcggcgac aacgcgatga ccgacgaccc gctgctcgtc gactatgcgc
     2701 tcgacatcag cgcggacttc catctcgcgg ccggtagccc gatgatcgat gcgggcagcg
     2761 ccgagttcgc tccggccctc gacttcgacg gcaaggcccg gccgcagggc gacgccccgg
     2821 acgtcggccc gttcgagcat tgatcgccgg ccgcgcttcg cgggtgcttc gaccgcgggg
     2881 cgctaggcga tcgccgtcgc cggcgatccg tgaggcagcg agcgctcagg gcgtgcgagc
     2941 gagccaggcg ttgcgcagtc cgagggtgcg cggcggggcg tcggcggcga gctcgaagcg
     3001 aaagctgcgg tgcgggtcgg cggtgacggt cacggtgcgc agctcgaggc ggccgccgcg
     3061 gaacaccgcg acctcgacgc gctcgccagg cttgcgctcg gagatccgcg cgagcgcctc
     3121 gtcgctgctg cgcacgcgct tgcccgcgac ggcgatcagc tcgtccccca gcatcagccc
     3181 cgcggcggcc gcgggcgagg ccggctcgat gttccggatc gtgcgctcgg gcgtcagcac
     3241 gagccccgcg taggccttgc tcgcggcctc gctggtgacc tcgacgccga tccgcgcgag
     3301 cagctccggc agcggcagct cggccgtgcc atcgacatac ttcgaaaaaa atgcatccat
     3361 cggctcgccg gcctcggcgc tggcggccgc gacgatgtcg gcctgcgtca gtcccgcgcc
     3421 ggtctggccg tggctggcca tcaggcggcg aaacagcccg agcagcgagc cctgctgccc
     3481 gtgctcggca gaccgcaggc gcagctcgag gtcgaggcat acgcccaaca aatgtccctt
     3541 ctcataatag ctgatcagcg tattgccgtg cgcggggctc ggcttgtacg ccttgatcca
     3601 cgcgtcctgg ctgagatcgc cgagcggggt gtggttgcgg ccgggcttgc gcaggtaggc
     3661 ggtccaggcc tcgccgagct cgcgcacgtg ggtctccggt ggcacgatgt cggcccggac
     3721 gagggcctgc tgctccatcg tctcggtgaa gccctcgtgg aaccataaca ggcgggtgta
     3781 gttctcgcgg ctgtagtcgt aggggacgag ggcggcgtcg cgcaggcgct tgacgttcca
     3841 caggtggaag aattcgtggg cggcgaggtt ggcgaagcgg cggtagccgg ctggctccga
     3901 gaacatgtcc ctctggacac gtagcaatgt gctggtgccg tgctcgaggc cgccgtcgct
     3961 ggtctcgtcg gcgagcacga ggaagacgta gcggcgcagc ggcaggccgc ccatcaaacg
     4021 cgcgaaggtg ccgacgatct tctgcaggtc ggcggcgatc ctcgcggtgt cggcgttgct
     4081 gccggccggg gccgagaaca ccagctcgat gcgcgcgggg ccgacgtcga aggtgcgcag
     4141 ctcgggcgtg ccgagcacga tcggggcgtc gacgagggtg tcgtagtcgg gggcggtgaa
     4201 gttggcgctg gtcgtgctga tcgcgcccgc aggttcgagc gcgctgtggg cctgccaccg
     4261 cgccggcagc ttgacctcga cggccgcggg gcgcgaggtc tgaccgacga gatacatgaa
     4321 ggtcgagggg ccgttgaggc tggcgtggcg gtcgtcgacg aagctggtgc gcacgccggg
     4381 ctcgtcggcg aatacgcgat agcggacgcg aaagccccgg cggccgtggt cgacgcgcca
     4441 ggtctgcttg tcgacgcgct ggacgggcaa gggcttgccg tcgaggtcct cggcgcgcag
     4501 gccgtcgacg tggcgggcgt ggtcgcggac gaggtagctg ccgggggtcc acgcgggcat
     4561 ggcgacctgc gagcgggtgc ccttcgggcg cggcacggtc atcgtcacgt ggacccactg
     4621 ctgcgccggc gcggggatct cgagcgtgta atcgacctgg cgcggcgagg gggtttgcgg
     4681 gtgccgggtt tgactggaag ccgcggtagg agccgcggcg caggcggcga gggccgcggc
     4741 gaggacgagc ggcaggcgga tcatgcgggc ggcagtgtaa cagcggcagg cagatcaggc
     4801 gcgggcccgc agggtgctaa agcggacgct gcgctcgccg agctgcggcg cgggcggcga
     4861 gcccggggtc ggcctcgctg cggtgatgtc ggcggggatc ttgacgagga agacggtgcc
     4921 gacgcccggg gcgctgtcga cggtcaacac gccgcccatc agcttgagca ggccgagggt
     4981 gatcgtcagg ccgaggccgg tgccgccgta gcggcgagcg gtggcggcgt ccgcctgctc
     5041 gaagggctcg aagacgcggc cgatctgctc ggaggtcatg ccgatcccgc tgtcggcgac
     5101 gcgcaacacc agccacgggc cctcgcggtg gaccgcgagg ccgatctcgc cgtgctcggt
     5161 gaacttggcg gcgttggcga gcaggttgaa cacgatctgg cgcgtgcaca ccgggtcacc
     5221 gacgaccatg cgatcgtcgc cgagcaggac ctcgacgagc agggtgttgg accggcgctg
     5281 gatcgcgggc tccatcgtct cgaccagctc gcgcaggcac tgggcgaggt cgaagcggac
     5341 cgggtcgagg cgcagctgcc cggcctcgat cttcgagatg tcgaggatgt cgccgatcag
     5401 ctgcagcagg tggcgaccgg cggtctccat gcgctcggcg tcggcgacga tctgcgacat
     5461 gccgcgctcg cccgcctcgt cgcgcacgag ctcgacgtag ccgaggatcg cgttgagcgg
     5521 ggtccgcagc tcgtggctca tgtgcgcgag gaaggccgac ttcgccttgc tcgcctgcgc
     5581 ggccgcgtcg gcggcgctct gggccgcgac ggtctcgccg ctgcgtcgcg tcagctcgac
     5641 gttgctctgc atcagctcgg cctggcgctc ggcgagcgcg acgcgggccg tgcgtgcggc
     5701 gctgcgggcc agctcgcgcc cgcgtacggc ggcgcgggcg gccagcccga cgatcagcag
     5761 catggcggtc gggacgacga tcagcagcag gcgcaggtcg gtgtggggga tctcggcggg
     5821 atacagctgg ctgcgcgcca gcacgatcgc ggcccacagc gcgctgcagg gcagggtcca
     5881 gcgtagaaca ctgcgcgcgt cgaacacggt gctcaggccg atcgcgacgg cgagcgcggc
     5941 cggggtgagg ccgagcgcgg cgagcgtctc ggcggtcagg ccgacggcga tcgtggtggc
     6001 gagccagccg gcgatcgcga ggcgcgcggc ggcgaggctg cgatcgcgat gcagcagcag
     6061 gaccgcggcg ccgagcacga gcgcaccgcc gaggtaggcg aggccctcga cgaggtcgac
     6121 ggtcaggccg ctggcggacc agcgagcgag cgcccgcata gcgagggtcg cggtgacgcc
     6181 cgccgcgggg tagagcccgg ccggcagggc tcgcgcgcgc agagcatcgt ccgagagcag
     6241 gtcacgctcg ctggggatcc cgttggacat caaggagctg gccacgccag ccgggagcat
     6301 acgcagacgg cgcgacgttc gcagcgcccg agcggggccg gggcggggaa aatgtgcgtc
     6361 ccggccccgc gacagtgccc gggcggcccc gaacgcccgg gatcgcgcgc tgtgctccgc
     6421 gccccgcggg cgggctcaca tgtccttcgc gctgtagacg acctcgacga cgatcgaggc
     6481 cggcatgcgg tcgagggtcg cggggaactt ccaggtggag gcgatacggg tgatgcaggg
     6541 gccgatcggg ccgagctgcg tcgagcccca cttgagctgg gccgaggtga ccttgccggc
     6601 gccggcgacc ttgagcatca ggacgacgtg gccgacccgc tcggcgttgt cgaccgtctc
     6661 gcggaagcag cgcttgaggc cgtcggcctg cttgcgcagg ctctcccgga tcgtcagcgg
     6721 ggtcagctcg ccgacgacgt tcggggtctc gagctcgatc gtcacgacgc ggcgggcggc
     6781 ggcgatcgcc gcggcgctcg gcggggtgcg cttggcgtac atgtcgatct cgacgttctt
     6841 gccgggcgcc ttggtgggtt ttgggcggtc gtcgtgcagg ccgccgccgc gctcggtctc
     6901 ggcgagctcg aggccgccga ggggccggtt gatcaccggc gcgtcgcgga cggcgaccca
     6961 catggcgcgg tcggcctcgt tgtcgtagcc gagcagcccc tcctgggtgt cggcggtggc
     7021 gcgctggatc gaggcgttga actcggcgtc gttgagcacg ccggcgaccc gcgcataact
     7081 gtgatcgtca cggatcccgc ggcgcgtgcc cctggggacg atctgcgccg gaccggtgac
     7141 ctcgacggcg agctcgtcca cggtcgacgg ggccggcggc ggcgcggcgg gtttgggagg
     7201 agcgctcgac ggcctgtccc gtgggacagg tggggcgacg gccggcggag gcggcggggg
     7261 cgggggcacg tcggtgaggt actggacggc gagcaggcgc cgcgcgctct gctcctcgat
     7321 ctcgagctcg ccggtcgacg gctcgccgag caacagcagg gcgccgaggg ccgcgagggc
     7381 gccggcgttg ctgagcatgt acaggcgatc gaggcggccg cgcttcggca cgaaggcggc
     7441 gcgctcgacc gcctggacgg tgaagcgcag gtcgccgagg cgcagatcgg cgcgggcgtc
     7501 cgcgggcagg gcgtaggagc tgcggccctg ggcgacgagg tcggcgacgg cggtcgtctg
     7561 cgcgccgtcg tgcagctcgc cggtcatgcc gggcacgagg ccgaggacga tctgccgctc
     7621 gagcgcgcgg accagcggca cgccggcctg cgtcgcgccg ggcggcagcg cgacgggcag
     7681 gtgcacgtcc gggccctcgc cgatcacgta tctgtctctc gggacatgct gcgatctcgc
     7741 cgcgatcacc agcgggacga cgccgagcag gaccagcagg gcgccgaggc cagcggcgcc
     7801 ggggccgggc gcgggcgcgg gcgcgtcgac ggcctcggta tcggtgggct cgatccgggg
     7861 cgcgtcgggc aggctcgtca tcaccaggcc gaggccgagg accatcgcgc aggcgcccag
     7921 cgtgtaccag ccggcggccg ggacccgcgg ctcgggttcg acgccgacgt gctggatgtc
     7981 gacgacgcgc tcgccaaaat gcgcgacgac ctccgcgagg acggcgggcg gggtcgcggt
     8041 gacgggagac gcgggggaca ccacgggagg gtgacacgcc tgcggtccgc gaaagttccc
     8101 gatcgatcga ccttcgtgcg tgtgcgtcgt cgcgcaccgc gggcgcgggc tcccccgcgc
     8161 gcggcccccg tgatacacgc gaatgtatgt catgcgcccg cggttcgttg ttcctcgcgc
     8221 ctctgctgct ggtcgcctgc aattccggcg ggggcggtga aaccggcgag gccaccacgg
     8281 ggtcgtcggg gtcgtcgggg tcgtctgtcc ccggggacag ctcggggacg ggctcgtcgg
     8341 gtgggaccac ggtcaccacg gggtcggagc tcccgacgac gggcgagagc gagaccaccg
     8401 gcgatgtcag taccggcggc gtgacggtga cgggcagcga caccgatgtc ggcacgacgg
     8461 ggggcacgag cgacctgccc ggtgaggccg gctggcgcag cgtgctgtac ccggaggact
     8521 ggacgccggc gttcacgggc cccgagggcc gcttcctgca cgacttttca tacgccggtt
     8581 atcatctcgg cgagacgccg ctggcggtcg acgtgtcggc gaacaagatc gacgtcgtcg
     8641 ccgatcacgg ggccgacccg accggcacgg cggacgcgac ggccgcgttc caggcggcgc
     8701 tcgacgccgc ggggctggcc ggcggggtcg tcgaggtgcc ggcggggctt tataaaattg
     8761 aggggtcgct cgcggtcaag agctcgcgcg tggtgctgcg cggggtcggg gcggcgcaga
     8821 gcaagctgtg gttcacccgc ttcgagggca tgtcctataa gagtcatttg accttcggtg
     8881 ggccgctgga gctgacggat gagaccctgt tgacggccga cggcgagccg cgcaccaata
     8941 cggtgcaggt cgcggacgcg gcggcgttcg aggcgggcga cgacgtcgcg ctcgg
//
LOCUS       DASWNJ010001161         1771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_126160, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001161 DASWNJ010000000
VERSION     DASWNJ010001161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126160"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1710)
                     /locus_tag="VGB85_19585"
     CDS             complement(<1..1710)
                     /locus_tag="VGB85_19585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136301.1"
                     /translation="MLLEIPDLSHLLLRFPQMRDVLTESRCSVAGAAGRPLARGRCLH
                     GPQRIALGRWPRHDDVLLRFERTDPQLEYLLRAECMLRPGSSWLFRVASDRLAYESRG
                     MRVRADSKYILLSTEPFTGSSLARHVDLTCQGLHAVCLDVPSVFSPEWEQLLRQIQVT
                     QAKSIEVWPAGLAAVAWDGEGHGEWLASETPTLAIRSDHTIDELTIAMGKGPSLSLSL
                     TDMPPGEPIFIELPSLPVGLHKFSVAARNGTGADSEVIGDLDVVMRVREARPWSPGST
                     AHGPLDVELDPAAPSFEQLWEGKVSVLVRGPAGRQARCRVRMFVPAREAPLFERQLPP
                     VTLPLSSEEWTRHFGAHLKSDKGAPRAYDDAHVCEVEFSADELGTFTLRCEREFTPLR
                     WSVRRDSAGCIAHLHDDACSGNLAIERFSFERPTVGEKVPASAEHRAPRGGGLYVATI
                     GTFQAAIIVPPIVKGLADLRCEPKVDAGQRTPETIIKLMTFVQLWGRARLPGDLIAGA
                     RRDTVLRSLTAHIHALLGGAAWASAESDLKDERGLVYLKRSISDRKYEVGLGAGLHGE
                     YQSL"
BASE COUNT          319 a          596 c          586 g          270 t
ORIGIN      
        1 gagcgattga tactcgccgt gaagtcccgc gccaagccca acctcgtact tcctgtcaga
       61 gatactgcgc ttcaggtaga cgagcccacg ttcatccttg agatcagact ctgcgctcgc
      121 ccacgcggca ccacccagca gcgcatgaat atgcgcggtg agtgaccgga ggacggtgtc
      181 acgacgcgcg ccggcgatca gatcgccagg gagccgcgct ctcccccaga gctgcacgaa
      241 cgtcatgagc ttgatgatgg tctcaggcgt ccgttgcccc gcgtcgacct tcggctcgca
      301 acgaaggtcc gcgagcccct tcacgatcgg cggaacgatg atcgccgcct ggaaggttcc
      361 aatcgtagcg acgtagagtc ccccaccgcg aggcgcccgg tgctccgcgc tcgccggtac
      421 cttctctcca acggtgggcc gctcgaacga gaagcgctcg atggccaagt taccgctgca
      481 agcgtcgtcg tgcaggtggg cgatgcaccc cgcggagtct ctccgcacag accaccgcag
      541 cggcgtgaac tcgcgctcgc agcggagcgt gaaggtgccg agctcgtcgg cggagaactc
      601 aacctcgcag acatgcgcgt cgtcgtaggc gcgcggcgcg cctttgtcac tcttgaggtg
      661 cgccccgaag tgacgggtcc attcttcgct cgagagcggc aacgtcaccg ggggtagttg
      721 tcgctcgaaa agcggcgcct ctctggcggg cacgaacata cgcacccggc acctagcctg
      781 ccgtccagca gggccgcgga cgaggacgga gaccttcccc tcccagagtt gctcgaatga
      841 gggcgccgcg gggtccaact ccacgtcgag gggtccgtgc gcggtcgacc caggcgacca
      901 agggcgtgcc tctcgcacgc gcatgacgac atcgaggtcg ccgatgacct cggagtctgc
      961 gccggtcccg ttgcgcgccg cgacgctgaa cttgtgtagc ccgacaggga gcgacggcag
     1021 ttcgatgaag atcggctcgc cggggggcat gtcggtcagc gacagcgaga gcgaggggcc
     1081 tttgcccata gcgatggtca gctcgtcgat cgtgtggtcc gagcggatcg ccagcgtcgg
     1141 cgtctccgac gcgagccact cgccatgccc ctcgccgtcc cacgcgaccg ccgctaagcc
     1201 agcgggccac acttcgatcg acttcgcctg cgtgacttgg atctgcctga gcaactgttc
     1261 ccattcaggg gagaacacgg acgggacgtc gaggcacacg gcatgcagcc cctggcacgt
     1321 gaggtcgaca tggcgagcga ggctcgagcc cgtgaagggt tcagtgctca agaggatgta
     1381 cttcgagtcc gcgcggacgc gcatgccgcg cgactcgtat gcgaggcgat cactcgctac
     1441 ccggaaaagc cacgatgagc cgggacggag catgcactcc gcgcgcagca ggtactcgag
     1501 ctgtggatcg gtgcgctcga acctcagcag cacgtcgtca tgccgtggcc agcgaccgag
     1561 cgcgatgcgc tgcgggccat gaaggcagcg ccctcgcgcc aaaggtcgcc cggccgcgcc
     1621 cgcgacgctg cagcgtgact cggtaaggac gtcacgcatt tgagggaagc ggaggagcag
     1681 gtgcgagagg tctgggatct ccagcaacac ctcccagcac gaaccttcaa cgggcctgag
     1741 cacgacgcgc ggctcgatgc cgagccgcac g
//
LOCUS       DASWNJ010001162         2592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_91091, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001162 DASWNJ010000000
VERSION     DASWNJ010001162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91091"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2592)
                     /locus_tag="VGB85_19590"
     CDS             complement(<1..>2592)
                     /locus_tag="VGB85_19590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136302.1"
                     /translation="ASATGSPAVTVPATAIASSLRVESVKVRLTVHRAGERLPDGTKT
                     PHRGGMVDGEAEYTLTRPARPGERVILLNYAAAIPREPTELDEVAVSTYLDGPFRPAK
                     LELVDHSGAVAVQRVGPRGDLEIEPRPGTTLLRLFYRVSVTHRYWPMGCSRGRCSLDG
                     AVAPLPSVPAAGGPALPAGRVVDPVRWDVVEAKFATAPTWSPGQVPSASERRALNGDE
                     LVVTRTAVGGDGRLAYPSVFWGPRWHRSSQFYRGVKIEMLHMKRRPNNHVPDEYRAQL
                     RRDLPGHALRVARESLDVARAVGMEVPVGNTFMIVQGPVRTNVAEFHPSVVVISDQAL
                     QLWPGKRFLQFHTAAIARASLDLLTYGRQVGRHDPSTDLWLHGALTLGMLDIWRIQRE
                     HGDEYVSDIFRRFTFVPVVDNFLYSGQATFAQAYFRGSDDIMPLRVHPLYFSHDLPTG
                     RRIHEKLGDLMSVSQRAQLYIGLVSDPSADPRRLAESAYGRRLGWFFDQWLAPHPKLD
                     YSVRDVKSAEVGGKIRNRITIGRDGDSPLIEPVQVHVRERRGKSHYLVWNGDAEGHPS
                     TKPGEIPTRASHTFTLMTDRPLHSVTVDPRARLLETALPPHANVDPLYNNRHPSQFRF
                     IYSGVGFEVSASEFSAAQTNSARLQAFSGRVLFEASRRRDLRYTGHLQFQRDREAAAA
                     VGTGVSLWFGDKVNRRRRRARVRLFTELQYLNTAGLDQTGGVRVIENAAIMDDTRKFT
                     LWPDRGRRLLFGVAAGQTVRFGDKPDHRLSLTFQASWVQIWHLAHQHTLASRLEMAIM
                     SPLGTAPEYRSLIRVGGLEGLGGYGGNELFGQAMALAQLEYRHVYFNNLDANLGHVMW
                     LRGLGGAL"
BASE COUNT          384 a          885 c          956 g          367 t
ORIGIN      
        1 cagcgcgccg ccgaggccgc gcagccacat cacgtgcccg aggttcgcgt cgaggttatt
       61 gaaatatacg tggcggtact cgagctgcgc gagcgccatc gcctggccga acagctcgtt
      121 gccgccgtag ccgccgaggc cctcgagccc gccgacgcgg atcaggctgc gatactcggg
      181 ggccgtgccg agcggcgaca tgatcgccat ctcgaggcgg ctggcgagcg tgtgctggtg
      241 cgcgaggtgc cagatctgca cccacgaggc ctggaaggtc aggctgaggc ggtggtccgg
      301 tttgtcgcca aagcgcaccg tctggccggc ggcgacgccg aacagcaggc ggcggccgcg
      361 gtcgggccac agcgtgaact tgcgggtgtc gtccatgatc gccgcgttct cgatcacccg
      421 cacgccgccg gtctgatcga ggccggcggt gttgagatat tgcagctcgg tgaacaggcg
      481 cacgcgcgcg cgacggcggc ggcggttgac cttgtcgccg aaccacaggc tcacgccggt
      541 gccgaccgcc gcggccgcct cgcggtcgcg ctgaaactgc aggtggccgg tgtatcgcag
      601 gtcgcggcgg cggctcgcct cgaacaatac gcggcccgag aacgcctgca ggcgcgccga
      661 attggtctgc gccgccgaga actccgaggc cgacacctcg aagccgacgc cggaatagat
      721 gaagcgaaac tgcgaggggt gacgattgtt gtagagcggg tcgacgttgg cgtggggcgg
      781 cagcgcggtc tccagcagac gcgcgcgcgg gtcgacggtg accgagtgca gcgggcggtc
      841 ggtcatcaac gtgaacgtgt ggctcgcgcg cgtcggaatt tcgcccggct tggtcgacgg
      901 gtggccctcg gcgtcgccgt tccacaccag gtagtgcgac ttgccccggc gctcgcggac
      961 gtgcacctgc accggctcga tcaacggact atcgccgtcg cggccgatcg tgatgcggtt
     1021 gcggatcttg ccgccgacct cggcggactt gacgtcgcgc acgctgtagt cgagcttcgg
     1081 gtgcggcgcg agccattgat cgaagaacca cccgaggcgc cggccgtatg cggattcggc
     1141 gaggcgccgc ggatcggcgg agggatcgga gacgaggccg atgtacagct gggcccgctg
     1201 cgacaccgac atcaggtcgc cgagcttctc gtggatccgg cgcccggtcg gcaggtcgtg
     1261 gctgaagtac agcgggtgca cgcgcagcgg catgatgtcg tcgctgccgc gaaagtacgc
     1321 ctgcgcgaag gtggcttgcc ccgagtacag gaagttgtcg accaccggca cgaaggtgaa
     1381 gcggcgaaag atgtcgctga catactcgtc gccgtgctcg cgctggatcc gccagatgtc
     1441 gagcatgccg agggtcagcg cgccgtgcag ccacaggtcc gtcgacggat cgtgccggcc
     1501 tacctggcgc ccgtatgtca acagatcgag gctggcgcgg gcgatcgcgg cggtgtgaaa
     1561 ctgcaagaag cgcttgcccg gccacagctg cagcgcctgg tcgctgatga cgaccacgct
     1621 cgggtggaac tcggcgacgt tggtgcgcac cgggccctgc acgatcatga aggtgttgcc
     1681 gaccggcacc tccatgccga ccgcgcgggc gacgtcgagg ctctcgcggg cgacccgcag
     1741 cgcgtgcccg ggcaggtcgc gccgcagctg cgcgcggtac tcgtcgggca cgtggttgtt
     1801 cgggcgccgc ttcatgtgca gcatctcgat cttgacgccg cgataaaact gcgagctccg
     1861 gtgccagcgc gggccccaga acaccgacgg gtaggcgagc cggccgtcgc cgccgaccgc
     1921 cgtgcgcgtg acgaccagct cgtcgccatt gagcgccctg cgctcgctcg cgctcgggac
     1981 ctgtcccgga gaccaggtcg gcgccgtggc gaacttggcc tcgacgacgt cccagcgcac
     2041 cgggtcgacg acgcgaccgg ccggcagcgc cgggccgccg gccgcgggca cgctcggcag
     2101 cggcgcgacg gccccgtcga gcgagcagcg cccgcgcgag cagcccatcg gccaatagcg
     2161 gtgcgtcacg gacacgcgat agaacaggcg cagcagcgtc gtgccgggcc gcggctcgat
     2221 ctcgaggtcg ccgcgaggac cgacgcgttg caccgcgacc gcgccgctgt ggtcgacgag
     2281 ctcgagcttc gcgggccgaa acggcccgtc gaggtaggtc gagaccgcga cctcgtcgag
     2341 ctcggtcggc tcgcgcggga tcgcggccgc atagttgagc aagatgacgc gctcgccggg
     2401 ccgcgccggg cgggtcagcg tgtactcggc ctcgccgtcg accatgccgc cgcggtgcgg
     2461 cgtcttcgta ccgtcgggca ggcgctcgcc ggcgcggtgc accgtcagcc gcaccttgac
     2521 cgactcgacg cgcagggacg acgcgatcgc cgtcgccggc accgtgaccg ccgggctccc
     2581 cgtcgcgctc gc
//
LOCUS       DASWNJ010001163         1708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_513821, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001163 DASWNJ010000000
VERSION     DASWNJ010001163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513821"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1708)
                     /locus_tag="VGB85_19595"
     CDS             complement(<1..>1708)
                     /locus_tag="VGB85_19595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136303.1"
                     /translation="LLRMWPAIGGAQMRFLLPAGSEPGFVRIEARAVADREGPQRARI
                     YIARDDGPERAVDLWLPPADPSIVPIDAAVGVGPRASVVVGIGPDTRAVWVRVADNAP
                     RLALAASIRSTRDTELSAISKAEDATLLAAALPAPGDESPLDRLTRLSRAIREKPEDL
                     GLRLARAELLLDLDQPGYAFVDWRTVTGGRLSRALAARATALAERLDALDAPDSLDLT
                     TDSPVLVAPALAAAIGPAKARLQKIAPAIAAARKGGPSMGLRALDRLGFDDETRWRAV
                     GDPGRGGALIDAPDPADRSSGTGSKPGANNPGANNPGANKPATNKPARPGVVPIPDTA
                     EPTPVPADRPDPQRVSEPVGDLIAAAVLRAGWLDAQARPEEAARLWARLEARTGLWQA
                     GLNGVRSFLNVLDVETPAGVMPPPPKAEGSALAYGLALSLRRTIRTPPIQRLATVAAT
                     RSRWSRVEGSERDAGHEGLKVPRAPALPSPNAAVRQALVVPPWDPADATLLRPGYSTV
                     LELKRAAPGTLGIDLWCQTVRPDLGEGGLPRVRITLDGATLFDEPIVVEKTSGTTVEA
                     LPE"
BASE COUNT          196 a          587 c          723 g          202 t
ORIGIN      
        1 cctccggcag ggcctcgacg gtggtgcccg aggtcttctc gacgacgatc ggctcgtcga
       61 acagggtcgc gccgtcgagg gtgatccgca cgcgcggcag gccgccctcg ccgaggtccg
      121 ggcgcacggt ctgacaccac aggtcgatgc cgagagtgcc cggcgcggcg cgcttgagct
      181 cgagcacggt cgagtagcct gggcgtaaca gggtggcgtc ggcggggtcc cacggcggga
      241 cgacgagcgc ctggcggacc gcggcgttgg ggctcggcag ggccggcgcg cgcgggacct
      301 tgaggccctc gtggccggcg tcgcgctcgc tgccctcgac ccgcgaccag cggctgcggg
      361 tcgccgcgac ggtcgcgagc cgctggatcg gcggcgtgcg gatcgtgcgg cgcagcgaca
      421 gcgcgaggcc gtaagcgagc gcggagccct cggccttggg cggcggcggc atgacgcccg
      481 cgggggtctc gacgtcgagg acgttgagga acgaccgcac gccgttgagc ccggcctgcc
      541 acagacctgt gcgagcctcg aggcgagccc acagccgcgc ggcctcctcg gggcgcgcct
      601 gggcgtcgag ccagccggcg cgcagcaccg cggcggcgat caggtcgccg accggctccg
      661 agacgcgctg cggatcgggg cggtcggcgg gcaccggcgt cggctcggcg gtgtcgggga
      721 tcggcacgac gcccggccgc gcgggcttgt tggtggccgg cttgttcgcg ccgggattgt
      781 tcgcgccggg attgttcgcg ccgggcttgc tacctgtccc ggaggacctg tccgcagggt
      841 caggtgcgtc gatcagcgcg ccgccgcggc cggggtcgcc gacggcgcgc cagcgggtct
      901 cgtcgtcgaa gccgaggcgg tcgagcgcgc gcaggcccat cgacgggccg cccttgcggg
      961 ccgcggcgat cgccggggcg atcttctgca acctcgcctt ggccgggccg atcgcggcgg
     1021 ccagcgcggg ggcgacgagc acgggcgagt cggtggtcag gtcgaggctg tcgggggcgt
     1081 cgagggcgtc gaggcgctcg gccaaggcgg tcgcgcgggc ggcgagggcg cgagacaatc
     1141 ggccgccggt gaccgtgcgc cagtcgacga aggcgtagcc gggctggtcg aggtcgagca
     1201 gcagttcggc gcgagcgagg cgcaggccga ggtcctcggg cttctcgcgg atcgcacggc
     1261 tcaggcgggt caggcggtcg agcggcgact cgtcgccggg cgccggcagg gccgcggcga
     1321 gtagcgtggc gtcctcggcc ttgctgatcg cgctgagctc ggtgtcgcgg gtcgagcgga
     1381 tcgaggcggc gagggccagg cgcggcgcgt tgtcggcgac gcgcacccac acggcgcggg
     1441 tgtcggggcc gatgccgacg acgaccgacg cgcggggacc gacgccgacc gcggcgtcga
     1501 tcgggacgat cgacgggtcg gcgggcggca gccacagatc gacggcgcgc tcggggccgt
     1561 cgtcgcgggc gatgtagatg cgggcccgct gcgggccctc gcggtcggcg acggcgcgcg
     1621 cctcgatgcg cacgaagccg ggctcgctcc cggccggcaa caggaagcgc atctgggccc
     1681 caccgatcgc cggccacatg cgcagcag
//
LOCUS       DASWNJ010001164         3582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_198378, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001164 DASWNJ010000000
VERSION     DASWNJ010001164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198378"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3582
                     /locus_tag="VGB85_19600"
     CDS             <1..>3582
                     /locus_tag="VGB85_19600"
                     /inference="COORDINATES: protein motif:HMM:NF024820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0136304.1"
                     /translation="VAGTPRAGTLILLAILQQMVGAAIDPNAKINLLKLRAELREKQL
                     ADTQGAMAEWLRVLQIHPGSEEARFELDRLAERDNAWHLVLLVPAWELAQKPGKRDQT
                     RLLNQLADLYENNLGRPEYALRARTSAWRLNAASVADLPPRAGELGPQHARLWKLAEL
                     TGAYVSPPPPRDPLLWPHCNPPELADLAQWQKLGLHPITFARLTPAKTAAREPSDIMD
                     LSEVEIVDETRFSGEIVLEPEPTSFRSQREPSGFMELSEVEELDDARLTGEIDLDADQ
                     RAALRPRREQTGVVELIEIQELDDFEDARLTGEISLSPAEMQALRPRREQTSIVDINE
                     LEELDESGRGPTTRGGHTNIVDIADLMEDDGEDELIDTSSVPAVRPGQPALPRPQAKP
                     ARPSTPPMSTTRLPGRTGPSIPGPEARSKPPGKPTTSPTSPPRASQTTPAPSTHATPS
                     TSVPSAPAPSAAAVAAHRARVEAVTAGLPPLPELAGPALPARPAVHSAWEELALAYAS
                     TPATSKPDKASVAAALARLWDEGAHQPEPALLQLEAALTLTPDDTDLRSRLEDLAARE
                     QLPHRVIDAYSRMIGELTVPEHVVGYGLRLADLYERTGQLDRAEEQYQAVVSVAPRDR
                     DALRALARIHERSGREREYVDASARLLDLEAPELDVETRIGRTLALAQDLVIRVDRLV
                     DATRRLEALAREFPAHPQVQERHADLLIRQGLWTRAVEALRASAATVPDPAFALRATA
                     RIANLCEEHLAQVPQAIAAWQEIHQRRPDDAEALAQLQRLYLKTEQYGPLLPIVEQRL
                     AHVAPDDRDARIALLVVKARALQEGLGDEAAAMATLETLAAEAPENDDVLLGLSRLVR
                     RRGRLDEGLQLLRRRLDDAITAAFQVSGGPLTDDAETPDPFKPLPVDPFGPIPEGEAV
                     DPRIAAAARIVKIAGALADALEHEANEPQTALATIDEALLAIPAEEETNGPGRAALVA
                     RKVALARAQGDAAALIDGLSSLAHPDGLLEAAALARSALGDRELATQLYTRVLATRNP
                     SDPQQGKRLSSAIEGLVRLRIEAGDIDGADALMDAQLAELADTELRARILTEIGRTLL
                     ASLAHASTQLTTSEPEPEPTPDDELGESREPTMMYDLGDAPEPTGSYALADSGDAEPG
                     TLVGVRPETDDIEEIGEAEPDEDIAEELTELTDDDQEPLTLLPQARN"
BASE COUNT          543 a         1515 c         1147 g          377 t
ORIGIN      
        1 acgtcgccgg cacgccccgc gccggcacgc tgatcctgct ggcgatcctg caacagatgg
       61 tcggcgccgc gatcgacccc aacgccaaga tcaatttatt aaagctgcgc gccgagctgc
      121 gcgagaagca gctcgccgac acccagggcg cgatggccga gtggctgcgc gtgctgcaga
      181 tccaccccgg cagcgaggag gcccgcttcg agctcgaccg cctcgccgag cgcgacaacg
      241 cgtggcacct ggtcctgctc gtgcccgcgt gggagctggc ccaaaagcca ggtaagcgcg
      301 accagacccg cctgctcaac cagctcgccg acctctacga gaacaacctc ggccgccccg
      361 agtacgccct gcgcgcccga accagcgcct ggcgcctcaa cgccgccagc gtcgccgacc
      421 tcccgccgcg cgcgggcgag ctcggcccgc agcacgcccg cctgtggaag ctcgccgagc
      481 tgaccggcgc ctacgtgagc ccgccgccgc cgcgcgaccc gctgctgtgg ccgcactgca
      541 accctcccga gctcgccgac ctcgcgcagt ggcaaaagct cggcctgcac ccgatcacct
      601 tcgcgcggct gaccccggcc aagaccgccg cgcgcgagcc ctcggacatc atggacctgt
      661 ccgaggtcga gatcgtcgac gagacccgct tcagcggcga gatcgtgctc gagccggagc
      721 cgacgagctt ccgctcacag cgcgagccgt cggggttcat ggaattgtcc gaggtggagg
      781 agctcgacga cgcccgcctc accggcgaga tcgacctcga cgccgaccag cgcgccgcgt
      841 tgcgaccgcg ccgcgagcag accggcgtcg tcgagctcat cgagatccag gagctcgacg
      901 actttgaaga tgcccgcctc accggcgaga tctcgctcag ccccgccgag atgcaggccc
      961 tgcgaccccg ccgcgaacag accagcatcg tcgacatcaa cgagctcgag gagctcgacg
     1021 agtccggccg cggcccgacc acccgcggcg gccacaccaa catcgtcgac atcgccgacc
     1081 tgatggagga cgacggcgag gacgagctga tcgacacgtc gtcggtgccc gcagtccgcc
     1141 ccggccagcc cgccctgccg cgcccgcagg ccaagcccgc gcgaccgtcc acgccgccga
     1201 tgagcaccac acgcctgccc ggaaggacag gtccgagcat acctggcccc gaggccaggt
     1261 caaagccccc cggcaagccg accacctcgc ccaccagccc gccgcgcgcg agccagacga
     1321 cccccgcgcc gtcgacccac gcgactccct cgacctctgt cccgtccgcc ccggccccct
     1381 ccgccgccgc cgtcgccgcg caccgggccc gcgtcgaggc ggtcaccgcc ggcctgccgc
     1441 cgctgcccga gctggcgggc cccgccttgc ccgcccgccc cgccgtgcac agcgcgtggg
     1501 aggagctcgc gctcgcctac gcgagcaccc ccgcgaccag caagcccgac aaggccagcg
     1561 tcgccgccgc gctcgcccgc ctgtgggacg agggcgccca ccagcccgag cccgcgctgc
     1621 tccagctcga ggcggcgctc accctcaccc ccgacgacac cgacctgcgc agccgcctcg
     1681 aggacctcgc cgcccgcgag cagctgccgc accgcgtcat cgacgcgtat agcaggatga
     1741 tcggcgagct gaccgtgccc gagcacgtcg tcggctacgg cctgcgcctc gctgacctgt
     1801 acgaaaggac aggtcagctc gaccgcgccg aggagcagta ccaggcggtc gtcagcgtcg
     1861 ccccgcgcga ccgtgacgcc ctgcgcgcgc tggcccgcat ccacgagcgc agcggtcgcg
     1921 agcgcgagta cgtcgacgcg agcgcccgcc tgctcgacct cgaggcgccc gagctcgacg
     1981 tcgagacccg catcggtcgc accctggcgc tcgcgcaaga cctcgtgatc cgcgtcgatc
     2041 gcctggtcga cgcgacccgc cgcctcgagg cgctcgcgcg cgagttcccg gcccacccgc
     2101 aggtccagga gcgccacgcc gacctcctga tccgccaggg cctgtggacg cgcgccgtcg
     2161 aggccctgcg cgcctccgcg gcgaccgtgc ccgaccccgc gtttgcgctg cgcgcgacgg
     2221 cccgcatcgc caacctctgc gaggagcacc tcgcccaggt cccgcaggcg atcgccgcct
     2281 ggcaggagat ccaccagcgc cgccccgacg acgccgaggc gctcgcccag ctccagcgcc
     2341 tctacctcaa gaccgagcag tacggcccgc tgctgccgat cgtcgagcag cgcctcgcgc
     2401 acgtcgcccc cgacgaccgc gacgcccgca tcgcgctgct ggtcgtcaag gcgcgcgcgc
     2461 tgcaggaagg gctcggagac gaggccgccg cgatggcgac gctcgagacc ctcgccgccg
     2521 aggcccccga gaacgacgac gtcctgctcg gcctcagccg cctggtccgc cgccgcggtc
     2581 gcctcgacga gggcctgcag ttgctgcgcc gccgcctcga cgacgcgatc acggccgcct
     2641 tccaggtgtc cggcggtccg ctgaccgacg acgccgagac ccccgacccg ttcaagccgc
     2701 tgcccgtcga ccccttcggc ccgatccccg agggcgaggc cgtcgacccg cggatcgccg
     2761 ccgccgcgcg catcgtcaag atcgccggcg ccctcgccga cgcgctcgag cacgaggcca
     2821 acgagccgca gaccgccctc gccacgatcg acgaggccct gctggcgatc cccgccgagg
     2881 aggagaccaa cggccccgga cgcgccgcgc tggtcgcccg caaggtcgcg ctcgcccgcg
     2941 cccagggcga cgccgccgcg ctgatcgacg gcctctcctc gctcgcgcac cccgacggcc
     3001 tgctcgaggc cgccgcgctc gcccgctccg cgctcggcga ccgcgaactc gccacccagc
     3061 tgtatacccg cgtgctcgcg acccgtaacc cgagcgaccc gcagcagggc aagcgcctgt
     3121 cctcggcgat cgaggggctg gtgcgcctgc gcatcgaggc cggcgacatc gacggcgccg
     3181 acgcgttgat ggacgcgcag ctcgccgagc tcgccgacac cgagctgcgc gcccgcatcc
     3241 tcacggagat cgggcgcacg ctgctcgcca gcctcgccca cgcatcgacg cagctgacca
     3301 cctccgagcc cgagcccgag ccgacgcccg acgacgagct cggcgagtcg cgcgagccga
     3361 ccatgatgta cgacctcggc gacgcgcccg agccgaccgg cagctacgcg ctcgccgact
     3421 ccggggacgc cgagcccggc acgctcgtcg gcgtccgccc ggagaccgac gacatcgagg
     3481 agatcggtga ggccgagccc gacgaggaca tcgctgagga gctgaccgag ctcaccgacg
     3541 acgaccagga gccgctcacc ctgctcccgc aggcccgcaa cg
//
LOCUS       DASWNJ010001165         5776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_479897, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001165 DASWNJ010000000
VERSION     DASWNJ010001165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479897"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1530
                     /locus_tag="VGB85_19605"
     CDS             <1..1530
                     /locus_tag="VGB85_19605"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY0136305.1"
                     /translation="PPGRPFQIFAGAIAIGSTAAIVGAYYITLAANFPPNALTQFFVV
                     LAALVLGSIVAVFMAHRRLSGALVGWLRDPGDEVSLAAWRQAINYPAHMSVAVGLSAV
                     AVSLLAAAITALSHADWRLGLHIAVGGLLAATLDAIFAWLYTDWATRPLRRAMAVRDP
                     MLPVTGPGIVSLGLAAKMTIVIVGVSLVGAVVTGTLAYREAEAAIASGQVGALALHLV
                     IVTATGLAVSLSGCLLVTRHTTEPLRELTTLVTELNPERYTQRGVPMSADEVGNLMAA
                     VNSMLGGLEERDFIKDAFSRYVTRQVSDVVLQGGLELGGELLTATILIADIRDFTSLS
                     ERMPPRQVVRVLNRYFTEMVEECMEHGGMIDKFIGDAMMVVFGAPVRQPPEQSALHAA
                     KAALGMLGRLDGLNRSLIADGLPALRIGMGIHTGEAIAGNIGAPQRLDYTLIGDVVNT
                     VARIESVCKEVGADILVSEATRLLLGERARVGEARVVQLKGKAQPTPVYPLLALDSPT
                     A"
     gene            1705..2343
                     /locus_tag="VGB85_19610"
     CDS             1705..2343
                     /locus_tag="VGB85_19610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136306.1"
                     /translation="MQRTPWTPDQAAVLVHALADAALDACAAHFPASEGYETRATQLH
                     GMVEGVRVCVSKGEFSAVVSTQCYLEPAAGSPQTREVALRQVAALRRGEPPVLPTIRF
                     LEIARPALVIIGGSALLFAMISALTASGPSGYFRLSLWMSFMIMMAPAVAWLLGARSS
                     VQMEALDAARSAFIHQDHALADHESRWRRLLHTMHAQQALVMQHRALPFRRG"
     gene            complement(2387..2827)
                     /locus_tag="VGB85_19615"
     CDS             complement(2387..2827)
                     /locus_tag="VGB85_19615"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0136307.1"
                     /translation="MIELRALDGACTGPDHLFAICLRGTATQVGELLVRLDHDDPGLV
                     AYAGHIGFEVLPAHRGHRHALRATRLVSPLARRHGFSELWLMTAPDNAASRRTLELLG
                     AQHVDTVEVPVDSDMRKLGFVQVRRYHWILPLAGDERGSTSAHG"
     gene            complement(2861..>5776)
                     /locus_tag="VGB85_19620"
     CDS             complement(2861..>5776)
                     /locus_tag="VGB85_19620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136308.1"
                     /translation="HRVHGPAGARRALPGARGPQRRRRLRARRVRHDARQRDYATNEQ
                     AASAPGDLYRQADSRGTPAGLEIGEVDLDGPRPEEGGNPISGAMAEDGKPAYGSKSGS
                     KSDSWGDAQVALRKQQKLREYDANTVVQTGAGVPAWNWRVVNLGWNGPVTRDQKMRLI
                     LVPPHLNLALAFIRVLFLTTLMLAVFGVFGRRRARVAGGRVGRVGPMIALLLAFGTGL
                     LSPATAQAQVPSSEVLNELRARLIEPPECLPNCVTSPRMRLEATERGLRVVQELHVAA
                     FVSVLLPGSAEQWLPTTVLVDGSPVTSGLARTPEGTLILELRPGRHEVVLEGPLPVRE
                     TIQIALPQKPHRVEAKARGWTVDGIHEDGLADDNLQLTRVSPQDAKAAPAELEVGTLP
                     PFVRLERSLQLGLSWEVTTRVERLTPKGAAVVLSVPLMQGESVTTADQRVDAGKVLVN
                     MAPDQDSLSWTSVLPITEQIKLTAAAGESWTEVWLVEVGPVWHVEHIGLPTVRQGEAG
                     LREWRPWPGEQVALTVTRPQGVPGQTLTIDSATLYLTPGLRAVDARLSLALRSSRGGH
                     HVVQIPEGALLQNVTSNGVRQTIGQDGRQVRLPVEPGSQSIELSWREAHDLRQRYAGP
                     LVDLGAPAVNVNVEITFPQSRWILLVGGPRLGPAVLFWSYVIMLLLAAAVLSRLRWTP
                     LRGHQWFLLGLGLSPIEVPLVMIVIGWFLAIAWRREHTAVPAWWYDLRQLLLVSWTII
                     ACLCLKEAIHQGLLGQPDMQIEGNGSYGNTLRWYQDRVDHVAGTGGLVPRPWVLSVSM
                     WWYRGLMLAWALWLAWLLVRWLPWAWHGFSTGGLWRPLTSPSRRHDPEHSASGQQPAA
                     GVILPHNSSASAAIFATSGNVLASVEAAPPPSPSDTLPSTRIGGGPLATRQRNSATTR
                     AISGSTGHSTLEIQETMDGGAPVRTRLTPPPVPSATNPPRKSGARGADVEEVTPDDD"
BASE COUNT          790 a         2095 c         2055 g          836 t
ORIGIN      
        1 ccgccgggcc gccccttcca gatcttcgcc ggcgcgatcg cgatcggctc gaccgcggcg
       61 atcgtcggcg cctactacat cacgctcgcc gccaacttcc cgccgaacgc gctgacgcag
      121 ttcttcgtcg tgctggcggc gctggtgctc ggatcgatcg tcgcggtgtt catggcccac
      181 cggcgcctca gcggcgcgct ggtcgggtgg ttgcgcgacc cgggcgacga ggtgtcgctg
      241 gccgcgtggc gccaggcgat caactatccg gcgcacatgt ccgtcgccgt cggcctgtcg
      301 gcggtcgccg tctcgctgct cgcggccgcg atcacggcgc tgagccacgc cgactggcgg
      361 ctcggcctgc acatcgccgt cggtgggttg ttggcggcga cgctcgacgc gatcttcgcg
      421 tggctctaca ccgactgggc gactcggccg ctgcgccgcg cgatggcggt gcgcgacccg
      481 atgttgccgg tcaccggccc cgggatcgtc agcctcggcc tcgcggccaa gatgacgatc
      541 gtcatcgtcg gtgtgtcgct ggtcggggcg gtggtcaccg gcacgctcgc ctaccgcgag
      601 gccgaggccg cgatcgccag cggtcaggtc ggggcgctcg cgctgcacct cgtcatcgtc
      661 accgcgaccg ggctcgccgt cagcctctcc ggctgcctgc tggtcacgcg acataccact
      721 gagcccttgc gcgagctgac caccctggtc accgagctca accccgagcg ttacacccag
      781 cgcggcgtgc cgatgagcgc cgacgaggtc ggcaacctga tggccgccgt caactcgatg
      841 ctcggcggcc tcgaggagcg cgattttatc aaagacgcgt ttagtcgcta cgtgacgcga
      901 caggtaagcg atgtcgtgtt gcagggcggc ctcgagctcg gcggggagct cctgacggcg
      961 acgatcctga tcgccgacat ccgcgacttc acgagcctgt ccgagcgcat gccgccgcgg
     1021 caggtcgtcc gcgtgctcaa ccgctacttc accgagatgg tcgaggagtg catggagcac
     1081 ggaggaatga tcgacaagtt catcggcgac gcgatgatgg tcgtgttcgg cgctcccgtg
     1141 cgccagccgc ccgagcagtc ggccctgcac gccgcgaagg ccgcgctggg catgctgggg
     1201 cgcctggacg ggctcaaccg ctcgttgatc gccgacggtc tacctgccct gcggatcggg
     1261 atggggatcc acaccggtga ggcgatcgcg ggcaacatcg gcgcgccgca gcgcctcgac
     1321 tacacgctga tcggcgacgt cgtcaacacg gtggcgcgca tcgagtcggt ctgcaaggag
     1381 gtcggcgccg acatcctcgt gtccgaggcg acccgcctgc tcctcggcga gcgcgcgcgc
     1441 gtcggcgagg cccgcgtcgt gcaactcaag ggcaaggccc agcccacccc cgtctatccc
     1501 ctgctcgcgc tcgactcgcc gacggcctga cccgctcgtg cgccgttgga gtgcgccgct
     1561 ggagcgagcc gcgagcccgc gcgcagggct cgcctgcgca cggctcgcaa acaccttcgt
     1621 gggcccccct gtggacgcgc tggccgctcg ggggtaactt ggattgtgat gggagacgac
     1681 gccgaactgc gagtcgcgtg ggccatgcaa aggaccccgt ggacaccgga ccaggccgcg
     1741 gtcctcgtgc acgcgctggc cgacgcggcg ctcgacgcct gcgccgccca cttcccggcc
     1801 tcggagggct acgagacgcg ggcgacgcag ctgcacggca tggtcgaggg tgtgcgggtc
     1861 tgcgtgtcga agggcgagtt ctcggcggtg gtcagcacgc agtgctacct cgagccggcc
     1921 gccgggagcc cgcagacccg ggaggtcgcg ctacgccagg tcgccgcgct gcgtcgcggc
     1981 gagccgccgg tgctgccgac gatccgcttc ctcgagatcg ccaggcccgc gctggtgatc
     2041 atcggcggca gcgccctgct gttcgccatg atctcggcgc tcacggcgtc gggccccagc
     2101 ggctactttc gtctctcgct gtggatgagc ttcatgatca tgatggcgcc cgccgtcgcg
     2161 tggctgctcg gggcgcgcag ctccgtgcaa atggaggcgc tcgacgcggc ccgctccgcc
     2221 ttcatccacc aagaccacgc gctcgccgac cacgagtcgc gctggcgccg gctgctgcat
     2281 acgatgcacg cccagcaggc cctggtgatg cagcaccggg ccctgccgtt tcgccgcggc
     2341 tgaccgctgg tgccagtccg cgccccagtt ttctccggca gcgagctcac ccgtgtgcgg
     2401 acgtcgagcc acgctcatca cccgcgagag gcaggatcca gtggtagcgt cggacctgca
     2461 cgaagccgag cttgcgcatg tccgagtcga ccggaacctc caccgtgtcg acatgctgcg
     2521 ccccgagcag ttcgagtgtc cgccgcgatg ccgcgttgtc cggggccgtc atgagccaca
     2581 attccgagaa gccgtggcgc cgcgccagcg gactcaccag ccgcgtcgcc cgcagcgcgt
     2641 ggcggtggcc gcgatgcgct ggtaacacct cgaagccgat gtgtccggcg taagcgacga
     2701 gcccggggtc gtcgtggtcg agacggacca gaagctcgcc gacctgcgtc gcggtgccgc
     2761 ggaggcagat cgcgaacaaa tggtccggtc ccgtgcaggc gccgtcgagc gcccgcaact
     2821 cgatcacctc gtcggcgagc tcgatggggg gaaccatggc tcagtcgtcg tcgggcgtga
     2881 cctcctcgac atcggcgccg cgggccccgc tcttgcgcgg cgggttggtc gcggacggca
     2941 cgggcggcgg tgtgaggcgg gtgcgcaccg gggcgccgcc atccatcgtc tcctggatct
     3001 ctagcgtcga gtgcccggtc gatccggaga tcgcgcgcgt cgtggcgctg ttgcgctggc
     3061 gcgtggcgag cgggccgccg ccgatgcggg tcgaaggcag cgtgtcggac gggctcggcg
     3121 gcggcgctgc ctccacgctg gcgagcacgt tgccgctggt cgcgaagatc gccgccgagg
     3181 ccgacgagtt gtgcggcaag atcaccccgg ccgccggctg ttgaccgctc gccgagtgct
     3241 cggggtcgtg acggcgtgac ggcgacgtca ggggccgcca caggccgccc gtgctgaagc
     3301 cgtgccaggc ccacggcagc cagcggacca gcagccacgc gagccacagg gcccacgcga
     3361 gcatcaggcc gcggtaccac cacatcgaca ccgacagcac ccagggacgc ggcaccaggc
     3421 cgccggtgcc ggcgacgtgg tcgacgcggt cctgatacca gcgcagcgtg ttgccatacg
     3481 acccgttgcc ctcgatctgc atgtcgggct gaccgagcag gccctgatgg atcgcctcct
     3541 tcaggcacag gcaggcgatg atcgtccagc tgacgagcag cagctggcgc aggtcgtacc
     3601 accacgcggg cacggccgtg tgctcgcggc gccaggcgat cgccaggaac cagccgatca
     3661 cgatcatcac cagcgggact tcgatcggcg acaggccgag gccgagcagg aaccactggt
     3721 gtccgcgcag cggcgtccag cgcaggcgac tgagcaccgc cgcggcgagc agcagcatga
     3781 tcacgtacga ccagaacagc accgccggtc cgaggcgcgg cccgccgacg agcaggatcc
     3841 accggctctg gggaaacgtg atctcgacgt tgacgttgac cgcgggcgcg ccgaggtcga
     3901 cgagcgggcc ggcgtagcgc tggcgcaggt cgtgggcctc gcgccagctg agctcgatgc
     3961 tctgcgagcc cggctcgacc ggcaggcgga cctggcggcc gtcctgaccg atcgtctggc
     4021 gcacgccgtt ggaggtcacg ttctgcagca gcgcgccctc ggggatctgg acgacgtgat
     4081 gcccgccgcg gctgctgcga agcgcgaggc tgaggcgagc gtcgaccgcc cgcagacccg
     4141 gcgtcaggta cagcgtcgcc gagtcgatcg tcagggtctg accgggcacg ccctgcgggc
     4201 gcgtcaccgt cagcgcgacc tgctcgccgg gccacggccg ccactcacgc aggcccgcct
     4261 cgccctggcg aacggtcggc aggccgatgt gctcgacgtg ccacaccggc ccgacctcga
     4321 ccagccacac ctcggtccac gactcccccg cggccgcggt cagcttgatc tgctcggtga
     4381 tcggcaggac cgaggtccag ctcagcgagt cctggtcggg cgccatgttg accagcacct
     4441 tgccggcgtc gacgcgctgg tcggcggtcg tcaccgactc gccctgcatc agcggcaccg
     4501 acagcaccac cgccgcgccc ttgggcgtca ggcgctcgac gcgggtcgtc acctcccacg
     4561 acagcccgag ctgcagcgag cgctcgagcc ggacgaaggg cggcagcgtg ccgacctcga
     4621 gctccgccgg cgccgccttg gcgtcctgcg ggctgacgcg cgtcagctgc aggttgtcgt
     4681 ccgcgagccc gtcctcgtgg atcccgtcga cggtccagcc cctggccttc gcctcgacgc
     4741 ggtggggctt ttgcggcaac gcgatctgga tcgtctcgcg gaccggcagc gggccctcga
     4801 gcacgacctc gtggcgaccc gggcgcagct cgaggatcag cgtgccctcg ggtgtgcgcg
     4861 cgaggccgct ggtcaccggc gagccgtcga cgagcacggt cgtcggcagc cactgctcgg
     4921 cggagcccgg cagcagcacg ctgacgaacg cggcgacgtg cagctcctgg accacccgca
     4981 gcccgcgctc ggtcgcctcg aggcgcatgc gcgggctggt gacgcagttg ggcaggcact
     5041 cgggcggctc gatcaggcgc gcgcgcagct cgttgaggac ctcggagctc ggcacctgcg
     5101 cctgcgccgt cgccgggctc agcagacctg tcccgaaggc caggagcagc gcgatcatcg
     5161 gtccgacgcg gccgacgcgg ccgcccgcga cgcgggcgcg acggcgcccg aacacgccga
     5221 acacggcgag catcagggtg gtcaggaaca gcacgcggat gaacgccagc gcgaggttga
     5281 ggtgcggcgg cacgaggatc aggcgcatct tctggtcgcg cgtgaccggg ccgttccagc
     5341 cgaggttgac cacgcgccag ttccaggcgg gcacgccggc gcctgtctgc acgaccgtgt
     5401 tcgcgtcgta ctcgcgcagc ttctgctgct tgcgcagcgc gacctgggcg tcgccccagc
     5461 tgtcggactt cgagccgctc ttgctcccgt acgccggctt gccgtcctcg gccatcgccc
     5521 cggagatcgg gttgccgccc tcctcgggtc gcggcccgtc gaggtcgacc tcgccgatct
     5581 cgagcccggc gggcgtgcca cggctgtcgg cctggcgata caggtcgccg ggggccgagg
     5641 ccgcctgctc gttggtcgca tagtcgcgct ggcgtgcatc atgccgaacg cgccgagccc
     5701 gaagtcgtcg tcgtcgctgc ggaccgcgag cgccgggtag agcccgccgc gcacctgctg
     5761 gaccgtgaac gcgatg
//
LOCUS       DASWNJ010001166         3418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_403726, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001166 DASWNJ010000000
VERSION     DASWNJ010001166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403726"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..1165
                     /locus_tag="VGB85_19625"
     CDS             80..1165
                     /locus_tag="VGB85_19625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136309.1"
                     /translation="MHRPALFVVVSLALFACDSGKPAEAGAKVEVAADAKAGAAVQGD
                     AKAEAEAAVPTVVVAGAVGAELRPVAEVEAALGTKISLAAADLDLAGVARLVATGKLK
                     AAAELELLLNAPGTAAHRIDLDADGKLDYVQVVELRAGSAMTFELRAVPSSKLDASLA
                     VLLGNIVVARAEAGGAKAGGGAKAGGELVVSANYAAAVAGGADFAFSQDVAAEIRGDV
                     VTVTDASAGAFVAWTFAAGRPVYVSTHVSTADIQLSADGAAHFAADASAKLDAAQLAA
                     LRAELKLAAPKLAVDVKADAGLKAGAKAEHGAKAGGSAKVEHSAKVEHSAKAEAGGLK
                     VGGSVKVGGGVSIGGGGKAGGGIKIGK"
     gene            1183..1650
                     /locus_tag="VGB85_19630"
     CDS             1183..1650
                     /locus_tag="VGB85_19630"
                     /inference="COORDINATES: protein motif:HMM:NF017936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0136310.1"
                     /translation="MSALVERDAAAVIAALGLVPDPDGGYFRDHRGAGKRAAGVKLRY
                     HLWTLAAPRRALHRVGGDCLYFYHAGGPLRVHTLGPGGDYTAELLGPPHTFQVAVAGG
                     RWRAIELVSGPWALISAAIVPGGAGHESADMTLRERVSPALWAILGSFVGDAT"
     gene            1647..2144
                     /locus_tag="VGB85_19635"
     CDS             1647..2144
                     /locus_tag="VGB85_19635"
                     /inference="COORDINATES: protein motif:HMM:NF017936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0136311.1"
                     /translation="MSRGEALIAALGMRPHREGGHFVERWRSTQEQATASGPRPLAST
                     IYYLLSSAAPVGRFHRNSSDITHFLHSGGPIVYCLISPEGGWREVVLGHDLAAGQVLT
                     FTCPGGWWKSSHLPAGVEHGLISEIVAPGFDYADHLIADEALFARLFPQLRARWAAYV
                     SPPGA"
     gene            complement(2125..3054)
                     /locus_tag="VGB85_19640"
     CDS             complement(2125..3054)
                     /locus_tag="VGB85_19640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136312.1"
                     /translation="MTYRPTTSLLLSALTATTLAACEPKGDLGEYTDTTADTGDSEAE
                     SGATTGASVGTSLTGSEGTGDTGDTSNTSNIGSEGSGDTGTGDTGDTGDTSDTGDACG
                     EPELDFQANFEITIVPPLPPADTKITADCTVDGLLVEANFVHVELGCGERDVTIKFNV
                     STSFKPLFSKGDALVLTYHQSMPFWINQWFALRTPAPGGGLVLGGIMADALLPLDDDD
                     STLFDPVHLTEVAGVCPEPVDCEDPYQRLALNVAYDQTNQQVLSGNSVSTGMLTSVRV
                     DLGRAVRHFDNDCGVSDTPPEWYNALIIMLPEG"
     gene            3147..>3418
                     /locus_tag="VGB85_19645"
     CDS             3147..>3418
                     /locus_tag="VGB85_19645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136313.1"
                     /translation="MSPLLFTLALHAAPHDIAVTWRGVEGCEQGPFEDSLAGYLAGGT
                     RQRTVAVTVAVERVAAAQWAATLELATGGATSTRRLTGSSCAELSSA"
BASE COUNT          434 a         1078 c         1379 g          527 t
ORIGIN      
        1 gcacgctgtc aacgacgggg gcgcggcccc ccccatgcgc agggttttcg cggcgccggg
       61 tttgaacgat actgcgggca tgcaccgccc cgcactgttc gtcgtcgtct cgctcgccct
      121 gttcgcctgt gactccggga agccggcgga ggccggggcg aaggtggagg tcgccgccga
      181 cgcgaaggcc ggggccgcgg tgcagggtga cgcgaaggcg gaggccgagg ccgcggtgcc
      241 gacggtcgtg gtcgccgggg cggtcggggc ggagctgagg ccggtcgccg aggtcgaggc
      301 cgcgctcggc acgaagatct cgctcgcggc ggcggacctc gacctcgcgg gcgtcgccag
      361 gctggtcgcc accggcaagc tcaaagcggc ggccgagctc gagctgctgc tcaacgcgcc
      421 cggcacggcg gcacatcgta tcgacctcga cgccgatggc aagctcgact acgtgcaggt
      481 ggtcgagctg cgggccggca gcgcgatgac cttcgagctg cgcgcggtgc cgtcgtcgaa
      541 gctcgacgcg tcgctggcgg tgctgctcgg caacatcgtc gtcgcgcgcg cggaggcggg
      601 cggcgcgaag gcgggcggcg gcgcgaaggc gggcggcgag ctggtggtct cggcgaacta
      661 cgcggccgcg gtcgcgggcg gggccgactt cgcgttctcg caggacgtcg cggcggagat
      721 ccgcggtgat gtcgtgacgg tcacggacgc gtcggcgggc gcgttcgtgg cgtggacctt
      781 cgcggcggga cgcccggtgt atgtcagcac gcacgtgtcg acggcggaca ttcagctgtc
      841 ggccgacggc gcggcacatt tcgcggccga cgcctcggcg aagctcgacg cggcgcagct
      901 ggccgcgctg cgggcggagc tgaagctcgc ggcgccgaag ctcgcggtcg acgtgaaggc
      961 ggacgccggg ctcaaggccg gcgcgaaggc ggagcacggc gcgaaggcgg ggggcagcgc
     1021 gaaggtcgag cacagcgcga aggtcgagca cagcgcgaag gcggaggccg gcgggctcaa
     1081 ggtcggcggc agcgtcaagg tcggcggggg tgtgtcgatc ggcggcggcg gcaaggcggg
     1141 cggcgggatc aagatcggga agtgatcttc gcggaggatc gtatgtcggc gctcgtcgag
     1201 cgtgacgccg cggcggtgat cgccgcgctc gggctggtcc ctgatccgga cggcggatat
     1261 tttcgcgatc atcgcggcgc gggcaagcgc gcggccgggg tcaagctgcg ctaccacctg
     1321 tggacattgg cagcgccgcg gcgcgcgttg catcgggtcg gcggcgattg tctgtacttt
     1381 taccacgcgg gcggaccgct gcgtgtccat acgctcgggc cgggcggtga ttatacggcc
     1441 gagctgctgg ggccgccgca cacgtttcag gtcgcggtcg cgggcgggcg ctggcgggcg
     1501 atcgagctgg tgtcggggcc gtgggcgctg atcagcgcgg cgatcgtgcc gggcggggcg
     1561 ggccacgaga gcgccgacat gacgctgcgc gagcgggtgt cgccggcgtt gtgggcgatc
     1621 ctcgggtcgt tcgtggggga cgcgacgtga gccgcggcga ggcgctgatc gccgcgctcg
     1681 ggatgcggcc gcatcgcgag ggcgggcact tcgtcgagcg ctggcggtcg acgcaggagc
     1741 aggcgacggc gagcggtccg cgaccgctgg cgagcacgat ctactatctg ttgagcagcg
     1801 cggcgccggt cggccggttt caccgcaaca gctccgatat cacgcacttt ctccacagcg
     1861 gcgggccgat cgtctattgc ctgatctccc cggagggcgg gtggcgcgag gtcgtgctcg
     1921 ggcacgacct cgccgcgggg caggtgctca cgttcacctg ccccggcggg tggtggaaga
     1981 gctcgcatct gccggcgggt gtcgagcatg gcctgatcag cgagatcgtc gcgccgggct
     2041 tcgattacgc cgatcacctg atcgccgacg aggcgctgtt cgcccgcctg ttcccgcagc
     2101 tgcgcgcgcg ctgggccgcg tatgtcagcc ctccgggagc atgatgatca gcgcgttgta
     2161 ccactccggg ggggtgtcgc tcacgccgca gtcgttgtcg aagtgacgca cggcgcgccc
     2221 gaggtcgacg cgcacggagg tcagcatgcc ggtcgagacg ctgttgccgg agaggacctg
     2281 ctggttggtc tgatcgtagg cgacgttcaa cgcgagccgc tggtacgggt cctcgcagtc
     2341 gaccggctcg gggcacacgc cggcgacctc cgtcaggtgc accgggtcga acagggtgct
     2401 gtcgtcgtcg tcgagcggga gcagagcgtc ggccatgatc ccgccgagca cgagaccgcc
     2461 gcccggggcg ggggtgcgca gggcgaacca ttgattgatc cagaacggca tcgactggtg
     2521 ataggtcagc acgagcgcgt cacccttcga gaacagcggc ttgaagctgg tgctgacgtt
     2581 gaacttgatg gtgacatcac gctcgccgca gccgagctcg acgtgcacga aattggcctc
     2641 gacgagcaaa ccgtcgaccg tgcagtctgc ggtgatcttg gtgtccgcgg gcggcagcgg
     2701 cgggacgatg gtgatctcga agttggcctg aaagtcgagc tcgggctcgc cgcaggcgtc
     2761 gccggtgtcg ctggtgtcgc ccgtgtcgcc ggtgtcaccc gtgccggtat cgcccgatcc
     2821 ctcggatccg atgttgctgg tgttgctggt gtcgccggtg tcgccggtgc cctcggagcc
     2881 ggtcaggctg gtgccgacgc tggcgccggt ggtcgctccg ctctcggcct cgctgtcgcc
     2941 ggtgtctgcg gtggtgtccg tgtactcgcc gaggtcgccc ttgggctcgc aggccgcgag
     3001 ggtcgtcgcg gtgagggcgg agaggagcag ggaggtcgtg ggtcggtaag tcatggcgat
     3061 tatttcccgg ctgtgccgag atccgtgagg cgcccgcgat cttttttgtc ggtccgcgag
     3121 cgggccggcg atgtaggctc tcgcgggtgt cgccgctcct gttcacgctc gcgctgcatg
     3181 cggcgccgca cgacatcgct gtcacgtggc gcggggtcga gggctgcgag caggggccgt
     3241 tcgaggacag cctcgcgggc tacctcgcgg gcggcacccg ccagcgcacg gtcgcggtga
     3301 cggtcgcggt cgagcgcgtc gcggcggcgc agtgggccgc gacgctcgag ctggcgaccg
     3361 ggggcgcgac gagcacgcgc cggctgacgg ggagctcgtg cgccgagctg tcgtcggc
//
LOCUS       DASWNJ010001167         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_618003, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001167 DASWNJ010000000
VERSION     DASWNJ010001167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618003"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /locus_tag="VGB85_19650"
     CDS             complement(<1..1472)
                     /locus_tag="VGB85_19650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEY0136314.1"
                     /translation="MAQPFDADTTRLERAAEAIESLIDVDNGFITDNFILGLMRFGSD
                     PDPVVPDTAVPGDVTGLRDGVKLDVPWYDELALDKQYFQCTDGGQIVAALAGLPAPPD
                     NVGSWTRGALEFTKAYLAQSEADHPQDKGKRGAAVIVYTTGTWTDATGAQKLAPDAAN
                     PASTASELLGLGMPTHVVNLGGAPGKLLADPLAKAGGTGQALDVDDLQGATDAFAALL
                     AALKDEELLPVCEPDSPRVMFMIDASSAMLNVGSMHGEPGQAAWDQVREALAGEASVL
                     QAVVGGHPLDEVGRFGVVAFGGNDPGDQTLVMQYDYCPQQRIAWGLDPASSCEAPGCV
                     DPYAPPPIVWTSQNGAEIDPPGFDQDVLSHMPRCDLDPQTPAACTGSGRFTHLGLERV
                     ANNLAAYRAQCLMPDSPEPCDAATPFINILITDGPYDSTDAQVQAQLVSLFDAGMVTR
                     VFGLGDAVDPSQLEAMAGWGSGGAFPASFVADGDDLADAIA"
BASE COUNT          218 a          548 c          508 g          227 t
ORIGIN      
        1 gcgatcgcgt ccgccaggtc gtcgccatcc gcgacaaagc tggccgggaa cgcgccgccc
       61 gagccccacc cggccatcgc ctccagctgc gagggatcga ccgcgtcgcc gaggccgaac
      121 acgcgcgtca ccatgcccgc gtcgaacaac gataccagct gcgcctgcac ctgcgcgtcc
      181 gtcgagtcgt acggcccatc ggtgatcaat atgttgataa acggcgtcgc tgcgtcgcac
      241 ggctccgggc tgtcgggcat cagacactgc gcccggtagg ccgcgaggtt gttcgccacg
      301 cgctcgaggc cgaggtgcgt gaagcggccc gagcccgtgc acgccgccgg cgtctgcgga
      361 tcgaggtcac agcgcggcat gtggctgagc acgtcctgat cgaagccggg cggatcgatc
      421 tccgcgccgt tttgggacgt ccagacgatc ggcggcggcg cgtacggatc gacgcaaccg
      481 ggcgcctcac acgagctcgc cgggtcgagc ccccatgcga tgcgctgctg cgggcaatag
      541 tcgtactgca tgaccagcgt ctggtcaccc ggatcgttcc ccccgaacgc cacgaccccg
      601 aaacgaccga cctcgtcgag cggatggccg ccgacgactg cctgcagcac cgaggcctcg
      661 ccggcgagcg cctcgcggac ctgatcccag gctgcctggc ccggctcccc gtgcatggag
      721 cccacgttga gcatcgccga cgaggcgtcg atcatgaaca tcacgcgcgg gctgtccggc
      781 tcgcacaccg gcagcagctc ctcgtctttg agcgcggcga gcagcgccgc gaacgcgtcc
      841 gtggctccct ggaggtcgtc gacatcgagg gcctgcccgg tcccgccggc cttcgccagc
      901 gggtcggcga gcagcttgcc cggcgcaccg ccgaggttca cgacgtgggt cggcatcccg
      961 agcccgagca gctcgctcgc cgtcgacgcc gggttcgccg cgtccggcgc gagcttctgg
     1021 gcgccggtgg cgtcggtcca ggtgcccgtc gtgtacacga tgaccgccgc gccgcgcttg
     1081 cccttgtcct gcgggtgatc ggcctccgac tgcgcgaggt atgccttcgt gaattcgagg
     1141 gcgcccctgg tccagctgcc gacattgtcc ggcggcgccg gcaacccggc cagcgccgcg
     1201 acgatctggc cgccgtccgt gcactggaag tactgtttgt cgagcgcgag ctcgtcgtac
     1261 cacggcacgt cgagcttgac gccgtcgcgc agccccgtga catcgcccgg gaccgcggtg
     1321 tccggcacga ccgggtcggg atcggagccg aatcgcatca atccgaggat gaagttgtcc
     1381 gtgatgaagc cgttgtcgac gtcgatcagc gactcgatcg cctcggccgc ccgctcgagc
     1441 cgcgtcgtgt cggcgtcgaa gggctgcgcc atcgccgacg agtattcgag cgcgaacatc
     1501 a
//
LOCUS       DASWNJ010001168         5410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_232582, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001168 DASWNJ010000000
VERSION     DASWNJ010001168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232582"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..1275
                     /locus_tag="VGB85_19655"
     CDS             262..1275
                     /locus_tag="VGB85_19655"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0136315.1"
                     /translation="MTQLEGIAEHFPRGISGATRPKDPAAPVPAAPHAEAPDEPQAES
                     AAVKDPSVEASPVEAAEVETSPVEALPIKTTEVKASPVEAAEVEASPVEVAETEPVFA
                     SAVVEPPTAETASGALVDPLPRTLVSGSQDAEDTRSLQTTLDAEGQRSFIARAQVLGA
                     NAWRAVELGTGLGDIAISLARALPGLHLMAIDSSAAMLRLARRKQWMMNTNVTFTRGD
                     AQATGLVDRSCDLVLAHGVVHHLDDPAALFEEIARIAGDDAAIYICDLQRPETPEALE
                     RAVEAQAEEWSERQRRRLADAMRASLRVDEVRALCVAAGLTGVEVRLSGEHRWEVLRA
                     RRR"
     gene            1406..2251
                     /gene="ligD"
                     /locus_tag="VGB85_19660"
     CDS             1406..2251
                     /gene="ligD"
                     /locus_tag="VGB85_19660"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEY0136316.1"
                     /translation="MLTSQHKPMFPDDGISKGELVDYYRDVAPIMLPHVHDRPLTLQA
                     YPSGIAGMKYYQKNIGEKFPEWMARVTMLSKDEGETTYVLANEAATIVYLANQNCITF
                     HVCSVKPPRLDRPDLLIIDLDPSDNDFEKVREGALALRPMFTEMGLVPYVKTSGSRGL
                     HVVAPIVPRDDQAAVHNVGKALADRLVALAPDRFTVEMTKKRRGDRVFVDYLRNGPAQ
                     TVAAPYTVRGLPTAPVATPLLWDELEDPRLHARSFTLRNVRERLERLGDPWRDMMRDA
                     RALED"
     gene            complement(2363..4906)
                     /locus_tag="VGB85_19665"
     CDS             complement(2363..4906)
                     /locus_tag="VGB85_19665"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0136317.1"
                     /translation="MDGSTSSPTTVTPTTGTPTTEPTPTGSGDGGESTSTTDPTEDTT
                     TGDTTTGDTTSADPFCGDGQIDAGEVCDDGPNNGPGQACNAMCQTNACGDGDAGPGEQ
                     CDDGADNGDMNSCKLDCTNNVCGDGAVGPGEGCDDGNDIDDDECGDDCVPATCGDGKV
                     APTEQCDDGNQVDTDACTSACTNAVCSDGFVQPTLGEQCDAGVDNADNAACTTQCTSN
                     VCGDGALYNTGDGTEACDNGADNGPGQVCNDLCVLNTCGDGDAGPDEECDDGNNAPAD
                     GCSATCKLEGCGNSIVDPGEDCDDGKDGDNDDGCTDECKAPACGDGFLQASQGEQCDQ
                     GGANSDSGACTTMCKAAACGDGLIQQNVEQCDDGANNGAGKSCNAMCKANICGDGDPG
                     PGEACDDGNQSNDDACTNVCKQATCGDGFKQPGEGCDLGANNSNTGACTLVCQLPKCG
                     DTFLQMSNMEECDDGNQSNADMCVGMCKAAKCGDSFVQQGVEQCDDANAVNTDACLST
                     CKSAGCGDGFVQAGVEACDDANAVSTDACVACKAAKCGDGFVQAGVEACDDGNMVDND
                     ACSNTCKAPLIDLTFTPCGQTMQNGPTQAMCDAAYPPGNPLNGKVTVTLGIQAWTVPS
                     TGTYRIEAWGAEGGTQQNYGPGGKGAHVRGDVALMQGEVIRILVGQKGKTGTIYDIGG
                     GGGSFVVKQAGNVPILVAGGGGAPGNCGAGNIPAQNGKGADGNGTGGTSGNDGNWCGC
                     GGDGSAGGGFSQDGGGNGGKSFLNGGKGADTQRPNQCVNAGLGGFGGGGNGGNGGPGG
                     GGYQGGNAGGSGAGGLPYGTGGRSLTTGLNKVQEDGIKAGDGQVKIILL"
BASE COUNT          726 a         1943 c         1853 g          888 t
ORIGIN      
        1 gctcgcgccc ttcggcaaga cggtgctcac cgtcgaggag ctcgcgcgac tgcgcgagcc
       61 ctataccctc gtcgtcccgg cgctcggcgc accgcggcgg tgagcccgac cggcgaggaa
      121 cacgcagaat cgccgcaccc gcagacgcgt gaccgcataa cgtcgcgctg atgcgcgcgc
      181 gcccgcagca gcgagatcga acgttgacgc gctccaacct tggttgatag agcttctgat
      241 tcgatcgctg ggaggggtgc gttgacgcaa ctggaaggca tcgccgaaca tttcccgcgc
      301 ggcatttcag gcgcgacgcg gcccaaggat cccgcagcgc cggtcccggc cgctccgcac
      361 gccgaggcgc cggacgagcc gcaggccgag agcgcggcgg tcaaggatcc gtcggtcgag
      421 gcatcgcccg tcgaggcagc ggaggtcgag acatcgcctg tcgaagccct gccgatcaag
      481 accacggagg tcaaggcatc gccggtggag gcagcggagg tcgaggcatc gcccgtcgag
      541 gttgccgaga ccgagcccgt attcgcgagc gcggtggtcg agcctccgac cgccgagact
      601 gcatccggcg cgctcgtcga cccgctcccg aggacgctgg tgtcgggctc gcaggacgcc
      661 gaggacacgc gctccttaca gacgacgctc gacgccgagg ggcagcgcag cttcatcgcg
      721 cgggcgcagg tgctcggcgc gaacgcctgg cgggcggtcg agctcggcac ggggctcggg
      781 gacatcgcga tctctctggc gcgggcgctg ccggggctgc acctgatggc gatcgactcg
      841 tcggcggcga tgctgaggct cgcgcggcgc aagcagtgga tgatgaacac caacgtcacg
      901 ttcacccgcg gcgacgcgca ggcgaccggc ctcgtcgatc gttcatgcga cctggtgctc
      961 gcccacggcg tcgtgcacca cctcgacgac cccgcggcgc tgttcgagga gatcgcgagg
     1021 atcgccggcg acgacgctgc gatctacatc tgcgacctcc agcggcccga gacgccggag
     1081 gcgctcgagc gcgcggtcga ggcgcaggcc gaggagtgga gcgagcgcca gcgccggcgc
     1141 ctcgcggacg cgatgcgtgc gtcgctgcgg gtcgacgagg tgcgggcgct gtgcgtcgcc
     1201 gccggcctga ccggcgtcga ggtgcgcctg tccggtgaac accgctggga agtcctgcgc
     1261 gcgcgacgtc gttaggtctg cgcgcatgcg cgcgtgcgcg atcgtacccg ttgatcggac
     1321 gcggcctgat ggtccagtct cgccgcggag gacctcgcaa ttggttgcag cgacaacgac
     1381 gatcacggtc ggtgagcgga gcatcgtgct cacgagccag cacaagccga tgttcccgga
     1441 cgacggcatc agcaagggcg agctggtcga ctactaccgc gatgtcgcgc cgatcatgct
     1501 gccgcacgtg cacgaccggc cgctgacgct gcaggcgtac ccctcgggga tcgccggcat
     1561 gaagtactat caaaagaaca tcggcgagaa gttcccggag tggatggcgc gcgtcacgat
     1621 gctcagcaag gacgagggcg agaccaccta cgtgctcgcc aacgaggcgg cgacgatcgt
     1681 gtatctcgcc aaccaaaact gcatcacctt ccacgtgtgc tcggtaaaac cgccgcgcct
     1741 cgaccggccc gacctgctga tcatcgacct cgacccgtcc gacaacgatt ttgaaaaagt
     1801 tcgcgagggg gcgctcgcgc tgcggccgat gttcaccgag atgggcctgg tgccgtatgt
     1861 gaagacctcc gggtcgcgcg gattacacgt ggtcgcgccg atcgtcccgc gcgacgacca
     1921 ggccgcggtg cacaacgtcg gcaaggcgct cgccgaccgc ctcgtcgcgc tcgcgccgga
     1981 ccgcttcacc gtcgagatga ccaagaagcg ccgcggcgac cgcgtgttcg tcgactattt
     2041 gcgaaatggc ccggcccaga cggtcgccgc gccgtacacg gtccggggac taccgaccgc
     2101 gcccgtggcg acgccgctgc tgtgggacga gctcgaagac ccgcgcctgc acgcccgcag
     2161 cttcacgctg cgcaacgtcc gcgagcgttt ggagaggctc ggggacccgt ggcgcgacat
     2221 gatgcgcgac gcccgggcgc tcgaggactg acctgtcgtt tcggacatcg cgcattcacg
     2281 aggtgccggg ccggatccgg cgggcgacga agcctgcccg cgcccgttca tcccgcgcgg
     2341 atgaacggcc gtgcacaccc gctcacagca ggatgatctt cacctggccg tcgccggcct
     2401 tgatgccgtc ctcctgcacc ttgttgaggc cggtcgtcag cgacctgccg ccggtcccgt
     2461 agggcaggcc accggcgccc gagccaccgg cgttgccgcc ctgataacca ccgccgccgg
     2521 ggcctccgtt gccaccgttg ccgccgccac cgaagccgcc gaggcccgcg ttgacgcact
     2581 ggttcgggcg ctgggtgtcg gcccccttgc cgccgttgag gaacgacttg ccaccgttgc
     2641 ccccgccgtc ctggctaaag ccaccacccg cgctgccgtc cccgccgcag ccgcaccagt
     2701 tgccgtcgtt gccgctggtg cctccggtgc cgttgccgtc cgcgcccttg ccgttttgcg
     2761 cggggatgtt gccggcgccg cagttgcccg gcgcaccgcc gccgccagcg acgaggatcg
     2821 ggacgttgcc ggcctgcttg acgacgaagc tcccgccgcc gccgccgata tcgtagatcg
     2881 tgccggtctt gcccttttgt ccgacgagga tgcggatcac ctcgccctgc atcagcgcga
     2941 cgtcaccgcg cacatgcgca cccttgccgc cggggccgta gttctgctgc gtaccaccct
     3001 cggcgcccca cgcctcgatg cgataggtgc cggtcgaggg caccgtccac gcctggatcc
     3061 ccagggtgac ggtgaccttg ccgttgagcg ggttgccggg cggataggcc gcgtcgcaca
     3121 tcgcctgcgt cgggccgttt tgcatggtct ggccgcaagg cgtgaaggtc aggtcgatga
     3181 gcggcgcctt gcaggtgttg ctgcacgcgt cgttgtcgac catgttgccg tcgtcgcagg
     3241 cctcgacgcc ggcctgcacg aagccgtcgc cgcacttggc ggccttgcag gcgacgcacg
     3301 cgtcggtgct gaccgcgttg gcgtcgtcgc aggcctcgac gccggcctgc acgaagccgt
     3361 cgccgcagcc ggccgacttg caggtgctga ggcacgcgtc ggtgttgacc gcgttggcgt
     3421 cgtcgcactg ctcgacgccc tgctgcacga agctgtcgcc gcacttggcg gccttgcaca
     3481 tgccgacgca catgtccgcg ttcgactggt tgccgtcgtc gcactcctcc atgttgctca
     3541 tctgcaagaa ggtgtcgccg cacttgggca gctggcacac gagcgtgcac gcgccggtgt
     3601 tgctgttgtt ggcgccgagg tcacagccct cgcccggctg cttgaagccg tcgccgcagg
     3661 tcgcctgctt gcacacgttg gtgcaggcgt cgtcgttgct ctggttgccg tcgtcgcagg
     3721 cctcgccggg gcccgggtcg ccgtcgccgc agatgttggc cttgcacatc gcattgcagg
     3781 acttgccggc gccgttgttg gccccgtcgt cgcactgctc gacgttttgc tggatcagtc
     3841 cgtcgccgca ggcggccgcc ttacacatgg tcgtgcacgc gccgctgtcg ctgttggcgc
     3901 cgccctgatc gcactgctcg ccctggctgg cctgcaggaa gccgtcgccg cacgcggggg
     3961 ccttgcactc gtcggtgcag ccgtcgtcgt tgtcgccgtc cttgccgtcg tcgcagtcct
     4021 cgccggggtc gacgatgctg ttgccgcagc cctcgagctt gcacgtcgcc gagcagccgt
     4081 cggcgggcgc gttgttgccg tcgtcgcact cctcgtccgg gcccgcgtcg ccgtcgccgc
     4141 aggtgttgag cacacacaga tcgttgcaca cctggccggg gccgttgtcc gcgccgttgt
     4201 cacacgcctc ggtgccgtcg ccggtgttgt acagcgcacc gtcgccgcac acgttgctcg
     4261 tgcactgcgt ggtgcaggcg gcgttgtcgg cgttgtcgac cccggcgtcg cactgctcgc
     4321 cgagcgtcgg ctgcacgaag ccgtcgctgc acaccgcgtt ggtgcacgcc gaggtgcacg
     4381 cgtcggtgtc gacctggttg ccgtcgtcac actgctcggt cggcgcgacc ttgccgtcgc
     4441 cgcaggtggc aggcacacag tcgtcgccgc actcgtcgtc gtcgatgtcg ttgccgtcgt
     4501 cgcagccctc gccgggcccg accgcaccgt cgccgcacac gttgttggtg cagtcgagct
     4561 tgcagctgtt catgtcgccg ttgtcggccc cgtcgtcaca ctgctcgccc ggcccggcgt
     4621 cgccgtcccc gcaggcgttg gtctgacaca tcgcgttgca ggcctgcccg gggccgttgt
     4681 tcggcccgtc gtcacagacc tcgcccgcgt cgatctgccc gtcgccgcag aacgggtcgg
     4741 cgctggtggt gtcgccggtg gtggtgtcgc cagtcgtggt gtcctcggtc gggtcggtgg
     4801 tcgaggtcga ctccccgcca tcacccgacc cggtcggcgt cggctcggtc gtcggcgtgc
     4861 ccgtcgtcgg cgtgaccgtc gtcggcgacg aggtgctgcc gtccatcgcc gtcgtcccac
     4921 ccgtcgaccc gccgctcgac gaacccccac tggtcgccgt gtccccgcct ccgcagccgg
     4981 tccacgccag ccacgacacc aaactcgcca cccgcaccac gctgcgccgt atcatcgcca
     5041 cccatctccc gcggaccaaa ctccgcgccg gcatcctaac cgcggagcgc agatcgtggt
     5101 agcgcgatcg gtcgggtgcg gacggatcga gcacgtgtgc agacgcggcg ctcgcagcga
     5161 actgcgagat gctgtccgat gagacagctc aggaagttga aggcgcgcgc gccggtgcgg
     5221 acgcgcgccg tccgaacgcg cttgttcacg aggtgacggg cggcgcgggc cgcagcacgt
     5281 cagcgatcgc gcgaagctgg gtgatgaggt cctcggtgac cgcatggagc tcgcagccga
     5341 ctcgcagcgg caggtggagc atcaggccct ccttgcccgt ttggagcacc cgcttcgcgt
     5401 cctggttggc
//
LOCUS       DASWNJ010001169         1773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_57343, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001169 DASWNJ010000000
VERSION     DASWNJ010001169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57343"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VGB85_19670"
     CDS             complement(<1..368)
                     /locus_tag="VGB85_19670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136318.1"
                     /translation="MRVALPLTLSLASLVAVLAVPQQADARPLSLIPDDRPWSRGTLM
                     PSFGLGGSFGSRGGGSLLIGAGLNYFFVNNLSVGLNLRNFTTFLPPGLKSEFPGIERQ
                     IPTNEFSMIPGMTAILYRSFR"
     gene            573..971
                     /locus_tag="VGB85_19675"
     CDS             573..971
                     /locus_tag="VGB85_19675"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEY0136319.1"
                     /translation="MKQHIDIITRLLLGAAYADKRLEGAEMSRIEQILRAVAGGEPDP
                     EQMELLRSFNPAAFNVHSAASMLVGLDPADKRKLLDLIASVNDADDELDLAEDAYLRH
                     VALGMGVSESDIADLTLDVLDDEEMFGMLQ"
     gene            complement(977..>1773)
                     /locus_tag="VGB85_19680"
     CDS             complement(977..>1773)
                     /locus_tag="VGB85_19680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136320.1"
                     /translation="HDFKSFHAALREHCDLGTGEFEFVDGIVHAPDHLTLCLGKFVDE
                     APYLSDYRWLDIFYKSTRHRAQDFLTAEDYFFRYDTECHWLTRTVPGLEHRLPRLLLG
                     KLILGSTNLIRWSNRLAPLMRRLKKRPDVVVDLFIPDRRFADFYKWYEREARFFPLWV
                     IPYRMPRVYPWVSDAHAARMADDLLIDCAIYGMPNSEPDRDWSAVFEEQTFRHDGVKT
                     LISRNHYDRARFFEIHDEARYVAAKQRLDPHALFHRGVFEKLHRVE"
BASE COUNT          317 a          562 c          636 g          258 t
ORIGIN      
        1 cggaagctgc gatacaggat cgcagtcatg ccggggatca tgctgaactc gttggtcggg
       61 atctgccgct cgatgcccgg gaactccgat ttgagccccg gcggcaggaa ggtcgtgaag
      121 ttgcgcaggt tgaggccgac cgagaggttg ttgacgaaga agtagttgag cccggcgccg
      181 atcagcaggc tgccgccgcc gcgactgccg aacgaaccgc cgaggccgaa gctcggcatc
      241 aaggtgccgc gagaccacgg ccggtcgtcg gggatcaatg acagcgggcg cgcgtcggct
      301 tgctgcggga ccgcgagcac cgccacgagc gaggcgagcg agagcgtgag gggcagggcg
      361 acgcgcaagg ggacctccga acccgcagtg tcgcacgctc cgggacatgc gcacgcgtga
      421 gggcccgcca gcgggcaaaa gacccccgtg cacccctgtg acggggatcc agctccgggc
      481 acatgcgcgc cacgccccgc gccccggggc caggatctgg gctaggctgg gtccgtcgcc
      541 gccggcccgg cggcgggtaa gggaagaccg cgatgaagca gcacatcgac atcatcaccc
      601 ggctcctgct gggagccgcc tacgccgaca agcgcctcga gggcgccgag atgagtcgca
      661 tcgagcagat ccttcgcgcg gtggccggcg gcgagccgga ccccgagcag atggagctgc
      721 tgcgcagctt caacccggcc gccttcaacg tgcacagcgc cgcctcgatg ctcgtcggcc
      781 tcgaccccgc cgacaagcgc aagctgctcg acctgatcgc cagcgtcaac gacgcggacg
      841 acgagctcga cctcgccgag gacgcctacc tgcgccacgt cgcgctcggc atgggcgtct
      901 ccgagagcga catcgccgac ctcaccctcg acgtgctcga cgacgaggag atgttcggga
      961 tgctccagta gagccgctac tcgacgcggt gcagcttttc aaacaccccg cggtggaaca
     1021 acgcgtgggg gtcgaggcgc tgcttggcgg cgacgtagcg ggcctcgtcg tggatctcga
     1081 agaagcgggc gcggtcgtag tggttgcgtg agatcagggt cttgacgccg tcgtggcgaa
     1141 aggtctgctc ctcgaagacc gccgaccagt cgcggtccgg ctcgctgttc ggcatgccgt
     1201 agatcgcaca gtcgatcagc aggtcgtcgg ccatgcgggc cgcgtgggcg tcgctgaccc
     1261 acgggtatac ccgcggcatg cgatacggga tcacccacag cgggaagaac cgggcctcgc
     1321 gctcgtacca cttataaaaa tcggcgaagc ggcggtcggg gatgaacagg tcgacgacga
     1381 cgtccggccg cttcttgagc cggcgcatca gcggcgcgag ccggttcgac cagcggatca
     1441 ggttggtcga cccgaggatc agcttgccga gcaacaggcg gggcagccta tgctcgaggc
     1501 cgggcacggt gcgcgtcagc cagtggcact cggtgtcgta gcgaaagaag tagtcctcgg
     1561 cggtcaggaa gtcctgcgcg cggtggcgtg tggacttgta aaaaatgtcg agccagcggt
     1621 agtcgctgag gtagggcgcc tcgtcgacga atttgccgag gcacagggtc aggtggtcgg
     1681 gcgcgtgtac gatgccgtcg acgaattcga actcgccggt gccgagatcg cagtgctccc
     1741 gcagcgcggc gtggaagctt ttaaaatcgt ggt
//
LOCUS       DASWNJ010001170         1975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_618040, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001170 DASWNJ010000000
VERSION     DASWNJ010001170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618040"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="VGB85_19685"
     CDS             complement(<1..1139)
                     /locus_tag="VGB85_19685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136321.1"
                     /translation="MLARFVPVVRLALPLCLLFACGDDAREDASSATGSGTGTLTNVS
                     GISIGTDTPTTGEGSISITATEGEEPKLDLPANDTEVVPGGGDCQGGGGMEEDYEFSV
                     IWIGNTGQGTVSKIDTTTAKELARYRTGPTDASDPSRTSVNLRGDVAVANRSGSITKI
                     AVNPARCVDSNGNGTIDTSQGPEDIRAWGEDECVLWHHDVGFPQGLDSNQGGPRAVAW
                     DSGDPALCYSDARLWIGWRDQPNTGVKLRRLNGETGAQEVTLDVPDWQCNWGHGTYGG
                     ATDKSGAFWALGTLQTLVHVDPKTFAVNRYEGPGSVVYGIALDGTGQPWLGGYDGRLW
                     RFDLAGKSFVDMGAMGPGALRGLAIDAEGFAWIAGNDPCVLVQYNT"
     gene            complement(1187..>1975)
                     /locus_tag="VGB85_19690"
     CDS             complement(1187..>1975)
                     /locus_tag="VGB85_19690"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY0136322.1"
                     /translation="VAGEALKSERKFLRTLRGVFARKYQLEQREGSSFTGMLLNGEAD
                     LPVYWIVRETALQRRASEGSETLVKTDAPIPARRSRYPFKRKVTIGNVQFYEDADGLL
                     MPAYAVGQSYKLRRPPGVGVEERWVHVDLSEQVLVAYQGDTPVFTTLVSTGREPGMTP
                     IGVHRLQSKHVATSMRDQPIEEDAYSIEDVPWTQYFHNSVALHGAFWHGGFGLVRSHG
                     CVNLSPSDARWLFGFTEPHLPDGWHAMSPGVGSFARGSAVVITE"
BASE COUNT          272 a          726 c          671 g          306 t
ORIGIN      
        1 gtgttgtact gcacgagcac gcacgggtcg ttgccggcga tccacgcgaa gccctcggcg
       61 tcgatcgcca gcccgcgcag cgcgcccggc cccatcgcgc ccatgtcgac gaagctcttg
      121 cccgcgaggt cgaagcgcca cagccggccg tcgtaaccgc cgagccacgg ctgcccggtg
      181 ccgtcgagcg cgatgccgta gaccacgctg cccgggccct cgtagcggtt gacggcaaag
      241 gtcttggggt cgacgtggac cagcgtctgc agcgtgccca gggcccagaa cgcccccgac
      301 ttgtcggtcg cgccgccgta cgtgccgtgc ccccagttgc actgccagtc gggcacgtcg
      361 agcgtgacct cctgggcccc ggtctcgccg ttgaggcggc gcagcttgac gccggtgttc
      421 ggttgatcgc gccagccgat ccacaggcgc gcgtcgctgt agcacagcgc cgggtcgccc
      481 gagtcccacg cgaccgcccg cgggccgccc tgattactgt cgaggccctg cgggaagccg
      541 acgtcgtggt gccacagcac gcactcgtcc tcgccccagg cgcggatgtc ctccgggccc
      601 tgcgaggtgt cgatcgtgcc gttgccgttg ctgtcgacgc agcgcgccgg gttgaccgcg
      661 atcttggtga tgctgcccga gcggttggcg accgcgacgt cgccgcgcag gttgaccgag
      721 gtgcgcgagg gatccgacgc gtcggtcgga ccggtgcgat agcgggccag ctccttcgcc
      781 gtcgtcgtgt cgatcttcga caccgtgccc tgcccggtgt tgccgatcca gatgacgcta
      841 aattcgtagt cttcctccat accgccgccg ccctgacagt cgccgccgcc gggcacgacc
      901 tcggtgtcgt tggccggcaa gtcgagcttg ggctcctcac cctcggtggc cgtgatcgag
      961 atcgacccct cgccggtcgt cggcgtatcg gtgccgatcg agatccccga gacgttggtc
     1021 agcgtgcccg tgccgctgcc cgtcgccgac gacgcgtcct cgcgcgcatc gtcgccacac
     1081 gcgaacagca gacacagcgg cagggccaga cggaccacgg ggacgaagcg agcgagcatt
     1141 ccgccacgat accgcgcgcg cgctgcgctg accacccgcc cgttcgctac tccgtgatca
     1201 cgaccgccga gccgcgcgcg aagctcccga cgccggggct catcgcgtgc cagccgtcgg
     1261 gcaggtgcgg ctcggtgaag ccgaacagcc atcgcgcgtc gctgggcgac aggttcacgc
     1321 agccgtggct gcggaccagg ccgaagccgc cgtgccagaa ggcgccgtgc agcgcgacgc
     1381 tgttatgaaa atactgcgtc cacggcacgt cctcgatcga gtaggcgtcc tcctcgatcg
     1441 gctggtcgcg catcgaggtc gccacgtgct tgctctgcag gcgatgcacc ccgatcggcg
     1501 tcatgcccgg ctcgcggccg gtcgagacca gggtcgtgaa caccggcgtg tcgccctgat
     1561 aggcgacgag cacctgctcc gagaggtcga cgtggaccca ccgctcctcg acgccgacgc
     1621 ccggcgggcg gcgcagcttg tacgactgcc cgaccgcgta ggcgggcatc agcaggccgt
     1681 cggcgtcctc gtagaactgc acgttgccga tcgtcacctt gcgcttgaac gggtagcgcg
     1741 accgcctcgc ggggatcggc gcgtcggtct tgaccagcgt ctcgctgccc tccgacgcgc
     1801 gccgctgcag cgccgtctcg cggacgatcc agtacaccgg caggtcggcc tcgccgttga
     1861 gcagcatgcc ggtgaagctc gagccctcgc gctgctcgag ctggtacttg cgggcgaaca
     1921 cgccgcgcag cgtgcggagg aacttgcgct cgctcttgag ggcctcgccc gcgac
//
LOCUS       DASWNJ010001171         8585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_657687, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001171 DASWNJ010000000
VERSION     DASWNJ010001171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 8585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8585
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657687"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VGB85_19695"
     CDS             complement(<1..193)
                     /locus_tag="VGB85_19695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136323.1"
                     /translation="MMDLPTPHDLHTAIARTLPDALQGYLDPALIVLAAAAIGVLVRF
                     TLVHWLRQLSLLNKSPYDEI"
     gene            401..1417
                     /locus_tag="VGB85_19700"
     CDS             401..1417
                     /locus_tag="VGB85_19700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136324.1"
                     /translation="MMRRLHRAPFACLALTAITCTGKPDAPAPVTATAPATPATPATP
                     ATPATPATPATPVPPAMPAVAIEPPPKGSCDVTGTWRLKLVFWDSACVAAEDVLVDLA
                     LAPGPANTVVGKLQPVGTVGMFAAVVPALRVTPYSAPGTKCGLRLELGARSGTHAELL
                     LGREREGLDGLAALWVTGATPCQDHASVFGEYIATEPAPWSALTRAGPWPKLSAAKPA
                     SAELTAAARKITATSIFGGAPAEDPRIKLRGSIVDYVGAVVETPGAVKLHEVPCTATS
                     DGRCVAIVGDPCRPGIHEGEDCEGMYLTVVVNTTTGALDRADAGGYPVESQADIEKRN
                     AEAP"
     gene            1468..1881
                     /locus_tag="VGB85_19705"
     CDS             1468..1881
                     /locus_tag="VGB85_19705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136325.1"
                     /translation="MHATNLLLSSIAGLLVIACSAAEPAPSDPSLPVIAQYSEASNAF
                     PTALIRGTVVEQDGCIRLRTSDDARGRLLMWQPSHRVHRNGERIEIVDGKGKVIARVG
                     ESIDLGGGEMPAIENYLKSPIPPACTGPYWMVGEP"
     gene            2060..2980
                     /locus_tag="VGB85_19710"
     CDS             2060..2980
                     /locus_tag="VGB85_19710"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0136326.1"
                     /translation="MAKLHGPDGQRLDAIAARCIVGRSRACELVLDARDVSSEHAAIR
                     WNGTAWELEDLGSRNGTFVDGQRLPPRTIRALERGRSIRFANTLGAWLFVDDAPPQPM
                     AINLVDRRMVHAGADALALPDRGAPEVWLRHTDLGQWVAEREDATAVRVEDQEVLLVG
                     GQPWRLYLPSGAPATWQGTAAVPGAGPLRLRFRVAADEEYIELVACHDERTIDLKARA
                     HHYPLLLLARARLADQAAGSPEDQQGWMLQDRLQAMLKVDANSLNVSIHRIRVQLTQA
                     GVPDAAHVVERRPGTRQLRLGVAAITIEPL"
     gene            complement(2995..4860)
                     /locus_tag="VGB85_19715"
     CDS             complement(2995..4860)
                     /locus_tag="VGB85_19715"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAHL domain-containing protein"
                     /protein_id="HEY0136327.1"
                     /translation="MASDPVFDEPGHAFGTTICSGPTSEVGRWPSTSAAGSVDDDPSE
                     CVDDVLARGAILGRYVILSRIGKGGMGVVYAAYDPDLDRKVALKLLLSHHGGGVDGRL
                     RLLREAQAMAQLTDPNVVAVYDVGAFGDRVWIAMEFIHGQTLSSWLKQRPRSWREVLP
                     VFLRAGDGLAAAHTAGLVHRDFKPDNVMIDETGRVHVMDFGLVRSDRSLTKDLPNPLS
                     DLRGLDALRAMLTHEGAMVGTPQYMAPEQWAGTNTDTRTDQFSFCVALWEALHGGPPF
                     AATTVDELVVAVLEGRLQKPANSRVPRWLNRAMLRGLQVDPGRRFPTTRALLDALGFG
                     GSRGQVWRILVGLCVAATVVAGVLVGQRLEHAQRAAHAVLREKVRGARDHSAAIVSHV
                     LGLRAGMLADYDPLALELRRLVELQVELRAVAQTVGGAHQPEVLALLDERDGAVGRTV
                     EQVERFKTQYSVLRQSSQVFPVLVAELMVAAPELDDRLDTLLRELLRFNNMADDEAFE
                     QIRAAMRDLVAASSSEVAHAQLELLRRHTELILEHRPAVDRLVREVAQLQADPSYEAL
                     ADAIVASDAATIGPDDALRWMLIVAGLGGLGGLGSIAAWLLLRARRRFDGAAPLR"
     gene            complement(4850..5782)
                     /locus_tag="VGB85_19720"
     CDS             complement(4850..5782)
                     /locus_tag="VGB85_19720"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEY0136328.1"
                     /translation="MLPPHLLVAARPGEPIRPIPPASELAGDERQVALGRRLFVDPAL
                     SQDGKTRCIDCHPFEHGGADPRPRSLGSGGMVGAVQAPSIFNLAFNHCYNWDGRTCDL
                     AAHALLPLANPKVMNMGSEQLVAKLAAEGDYAGRFAAAYPDGLTANNVAHALASYERT
                     LVTPNSPLDRYLLGDDDALAPGALAGYRLFKEIGCVSCHQGTNVGGNLFQRFGVLDQP
                     GAAADTTPDAYMGRERVTRRAEDRRVFRVPGLRNVALTAPYFHDASAATLPDAVRVMA
                     RRQLGHELTEEQLARLVEFLGALTGDQPEAPHGE"
     gene            complement(6109..6273)
                     /locus_tag="VGB85_19725"
     CDS             complement(6109..6273)
                     /locus_tag="VGB85_19725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136329.1"
                     /translation="MRSDRFVTLLRERTPDRGATVVGPVRWIEPPARPFILTAPSEAV
                     DMTQATPTTP"
     gene            complement(6273..7373)
                     /locus_tag="VGB85_19730"
     CDS             complement(6273..7373)
                     /locus_tag="VGB85_19730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136330.1"
                     /translation="MTPGPASPELVQGAVDPDRYVVTPGGEPERLRHNFFYRDGTPAH
                     ARAMLEAFVRDEFPALMAGADSGDAERVELAAERILQRVVFFTAAARGRPEVLRAYED
                     VARALPGEQVGIVIKLLAHAGDAETERFLRSSAELSKGQPAAALIAETLRGGVGPAPE
                     IEAATRAPITGGGQLDVRWIQFMATGDTVHLGDILAVLAWPDRVRDHLQALLAASPVD
                     AQAATLRALAPLGFRFDPRTHAPQNLDDLDLRAIMSDGRLDGPRFKSFVDALPAPLPP
                     EVAVAAATKASAMWSLASNAAEHPPVLAACEAALAKQTGAARLSLLEILAIAHERRGD
                     LAAAERAWSSYRELDPRRPGVAEHLEELATKR"
     gene            7630..7815
                     /locus_tag="VGB85_19735"
     CDS             7630..7815
                     /locus_tag="VGB85_19735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136331.1"
                     /translation="MPREPARPAAPRFTAQALTADLDPRICIVDVALAEKLQTRHTQT
                     FAIRREPGDERDVPNVV"
     gene            7784..8407
                     /locus_tag="VGB85_19740"
     CDS             7784..8407
                     /locus_tag="VGB85_19740"
                     /inference="COORDINATES: protein motif:HMM:NF012771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136332.1"
                     /translation="MTSATSRTSFEEDLAQLDDRGLAMIDSPVGPGAILVGKSSPVEG
                     GSLSPEELLRAIFGDAAGPVRDTSLRAPPWCTGEVSLARAGITTTRERTSRRADERGD
                     DVARLALLRELPRAVAGDHGRAVAQHGDGGPALILFCAELTRPLLGSTGRVPGHEEVA
                     AAHRGVAVQRTEGRAADQDGAGLVDDDAEARRGARLRVGRGGGRRLA"
BASE COUNT         1299 a         3044 c         3010 g         1232 t
ORIGIN      
        1 cgatctcgtc gtacgggctc ttgttgagca gcgacagctg gcgcagccag tgaaccagag
       61 tgaaccggac cagcaccccg atcgcagccg cggcgagcac gatcagcgcc ggatcgaggt
      121 agccctgcag cgcgtccggc aaggtccgcg cgatcgcggt atgaaggtcg tgcggcgtcg
      181 ggagatccat caacccccgc acatagcacc gatcggcgcc ggacgtttcg aaccaccgac
      241 gagaaccgcg tcccatcggc gtggacatct ccgcgacctc acggcccgct cacgcagcgc
      301 cacagcggcg tgacacggcg atcgcggccg gcgccagcga atatgagcgc gtgaatcacc
      361 gcactccacc cgccgggcgt gcgatgatgc cgcgaggttc gtgatgcgca gactccatcg
      421 agcacccttc gcctgtctcg ccctcacggc gatcacgtgc accggcaaac cggacgcacc
      481 cgccccggtg accgcgaccg ctcctgcaac acctgcgacg cccgcgacac ccgcgacgcc
      541 cgcgacgccc gcgacgcccg cgacgcccgt gccaccggcg atgcccgcag tagcgatcga
      601 accgccgccg aagggcagct gcgacgtcac cggcacgtgg cggctcaagc tcgtgttctg
      661 ggacagcgca tgcgtcgcgg cagaggacgt gctcgtcgac ctcgcgctcg cgcccgggcc
      721 cgcgaacacg gtcgtcggca agctgcagcc agtgggcacc gtcgggatgt tcgccgccgt
      781 cgtgcccgcg ctgcgggtca cgccctacag cgcccccgga acgaagtgcg gcctgcgcct
      841 cgagctgggc gcgaggtccg gcacgcacgc cgagttgttg ctcggccgcg agcgcgaggg
      901 tctcgacggc ctcgccgcgc tgtgggtcac gggcgccacg ccatgtcagg atcatgcctc
      961 ggtgttcggt gagtacatcg cgaccgagcc ggcgccgtgg tccgcgctca cgcgcgcggg
     1021 gccgtggccg aagctgtccg ccgcgaagcc agcgagcgcc gagctgaccg cggccgctcg
     1081 caagatcacc gcgacgtcga tcttcggggg cgcgcccgcc gaggatccgc ggatcaagct
     1141 acgcgggtcg atcgtcgact acgtcggtgc ggtcgtcgag acgccgggcg ccgtcaagct
     1201 ccacgaggtc ccgtgcaccg ccaccagcga cggccgctgc gtggcgatcg tcggcgaccc
     1261 gtgccggccc ggcatccacg agggcgagga ctgcgagggc atgtatctca ccgtcgtcgt
     1321 gaacaccacg accggcgcgc tcgatcgcgc cgacgccggc ggttatccgg tcgagtcgca
     1381 ggccgacatc gagaagcgca acgccgaggc gccctgaccc gggcgcgccc gttgtcgctg
     1441 acccggatcc atgcgaaaaa cgtcgtcgtg cacgcgacga acctgctcct gtcctcgatc
     1501 gccggtctgc ttgtgatcgc ctgctcggct gccgagcccg ccccgtccga tccgtcgctg
     1561 ccggtgatcg cccagtatag cgaggcgagc aacgcgttcc cgacggcgtt gatacgcggc
     1621 accgtcgtcg agcaggacgg ctgcatccgc ctccgcacca gcgacgacgc acgcggccgc
     1681 ctgctcatgt ggcagccgag ccaccgggtc caccgcaacg gcgagcgcat cgagatcgtc
     1741 gacggcaaag gcaaggtcat cgcgcgcgtc ggtgagtcga tcgatctcgg cggtggcgag
     1801 atgcccgcga tcgaaaacta tctcaagtcg ccgatcccgc cggcctgcac cgggccgtac
     1861 tggatggtcg gagagccgtg atcgggccct gaccccgcgc tcggcccacg tgcacgaggc
     1921 agtcgtggcc ccgccggctt caggaatgac gtgatgttga cggcgttagg caatgaaagc
     1981 tgggcaagcc ggaggagccg ggatatcctc gcgaaaggac gcgtcaggat cttcgccggt
     2041 cctcaagatc ggatcattta tggcgaagct ccacggtcca gacggtcagc gtctcgacgc
     2101 catcgccgcc cggtgcatcg tcgggcgctc gcgtgcctgt gagctcgtcc tcgacgcgcg
     2161 cgacgtctcc agcgaacacg cggcgatccg ctggaacggg accgcctggg agctggaaga
     2221 tctgggcagt cgcaacggca ccttcgtcga cggccagcga ctgccaccac gaacgatccg
     2281 ggcgctcgag cgcggcaggt cgatccgctt cgccaatacc ctcggcgcgt ggttattcgt
     2341 cgacgacgcg cccccgcagc cgatggcgat caacctcgtc gatcggcgga tggtgcacgc
     2401 tggcgcggat gcgctggcgc tccctgaccg cggcgcgccg gaggtatggc tgcgccacac
     2461 cgacctcggc cagtgggtcg ccgaacgcga ggatgccacg gcggtgcgtg tcgaggacca
     2521 ggaggtgctg ctcgtcggcg gccagccgtg gcggctctac ctgccctcgg gcgctccggc
     2581 gacctggcag ggcaccgcgg cagtgcccgg cgcgggtccg ctgcgcctgc gttttcgcgt
     2641 cgccgccgac gaggaatata tcgagctggt cgcgtgccac gacgagcgaa cgatcgacct
     2701 caaggcccgc gcccaccact acccgctgct gctcctcgcc cgggcgcgcc tcgcggacca
     2761 ggccgcaggg agccccgagg atcagcaagg ctggatgctg caagatcggc tgcaggcgat
     2821 gttgaaggtc gacgcgaaca gcctcaatgt cagcatccac cgaatccgcg tccagctcac
     2881 gcaagcgggg gtccccgatg cggcgcacgt cgtcgagcgt cgtccgggca cgcgccagct
     2941 ccggctcggc gtcgcggcca tcaccatcga gcccctgtga gccgcgcgcc aatgtcagcg
     3001 taggggcgcc gcgccgtcga accggcgccg agcccggagc agcagccacg ccgcgatgct
     3061 gccgagaccg ccgaggccgc cgaggccggc gacgatgagc atccagcgca acgcgtcgtc
     3121 gggcccgatc gtggcggcgt cgctggcgac gatcgcgtcg gcgagcgcct cgtagctggg
     3181 gtcggcctgg agctgcgcga cctcgcggac gagccgatcg accgcggggc gatgctcgag
     3241 gatgagctcg gtgtgtcgtc gcagcagctc gagctgcgca tgcgcaacct cggagctcga
     3301 ggctgcgacg agatcccgca tcgccgcgcg gatctgctcg aaggcctcgt cgtcggccat
     3361 gttgttgaag cgcaggagct cgcgcaggag cgtgtccagc cgatcgtcga gctccggggc
     3421 ggccaccatc agctcggcga ccagcaccgg gaacacctgc gacgactggc ggagcaccga
     3481 gtactgggtc ttgaagcgct cgacctgctc gacggtgcgg ccgacggctc cgtcccgctc
     3541 gtcgagcagc gcgagcactt cgggctgatg ggcgccgccg acggtctgtg ccacggcgcg
     3601 cagctcgacc tgcagctcca cgagacggcg gagctcgagc gccagcggat cgtagtccgc
     3661 gagcatgccg gcgcgcagcc ccagcacgtg actcacgatc gcagccgagt ggtcgcgggc
     3721 gccgcgaacc ttctcccgca acacggcgtg cgccgcccgc tgcgcgtgct cgaggcgctg
     3781 tccgaccagg acaccggcga ccaccgtcgc cgcgacgcac aggccgacga ggatgcgcca
     3841 gacctgaccg cgcgacccac cgaatccgag ggcatcgagg agcgcgcgcg tcgtcgggaa
     3901 gcgccggccc ggatcgacct gcagcccgcg cagcatcgcc cggttcagcc agcgggggac
     3961 ccgcgaattg gccggcttct ggagccgacc ctcgagcacc gcgaccacca gctcgtcgac
     4021 ggtcgtggcc gcgaacggag ggccgccgtg gagggcctcc cacagggcca cgcagaacga
     4081 gaactggtcg gtgcgcgtat cggtgttggt gcccgcccac tgctccgggg ccatatattg
     4141 cggcgtcccg accatcgcgc cctcatgcgt cagcatcgct cgcaacgcgt cgagcccgcg
     4201 gaggtcggag agcgggttcg ggaggtcttt cgtgagcgac cggtcggagc gcacgaggcc
     4261 gaagtccatg acatgcacgc ggccggtctc gtcgatcatc acattgtcgg gcttgaagtc
     4321 gcggtggacc aggcccgccg tgtgcgcggc cgcgaggccg tcgccggcgc ggagaaacac
     4381 gggtagtacc tctcgccagc tccgcggtcg ctgcttgagc cagctcgaga gcgtttgtcc
     4441 gtggatgaat tccatggcga tccatacccg gtcgccgaat gcgccgacgt cgtagacggc
     4501 gacgacgttg gggtcggtga gctgggccat cgcctgggcc tcacgcaaca gtcgcaggcg
     4561 cccgtcgacg ccgccgccgt ggtgcgagag cagcaacttg agggcgacct tgcgatcgag
     4621 atcggggtcg tacgccgcgt acacgacccc catgccgccc ttgccgatgc gtgacaggat
     4681 cacgtatcgc ccgaggatgg cgccgcgcgc caggacatcg tcgacgcact ccgacgggtc
     4741 gtcgtcgacg ctcccggccg ccgaggtcga gggccaccgg ccgacctcgc tcgtcgggcc
     4801 gctgcagatc gtcgttccga aggcgtgccc cggttcatcg aagaccgggt cactcgccat
     4861 ggggcgcctc cggctggtca cccgtcagcg cgccgaggaa ctccaccagt cgcgcgagct
     4921 gttcctcggt cagctcgtgc ccgagttggc gacgcgccat gacccgcacg gcgtcgggca
     4981 gggtcgcggc ggaggcgtcg tggaagtatg gcgccgtcaa tgcgacgttg cgcaggcccg
     5041 gcacacgaaa cacccggcgg tcctccgcgc gcctggtcac gcgctcgcga cccatgtatg
     5101 catccggcgt cgtgtcggcg gcggcgcctg gttgatcgag cacgccgaat cgctgaaaca
     5161 aattaccgcc gacgttggtg ccctggtggc agctgacgca cccgatctcc ttgaacaggc
     5221 gatatcccgc gagcgccccc ggagcgagcg cgtcgtcgtc gccgaggaga tagcggtcga
     5281 gcggggaatt cggggtgacc agcgtgcgct cgtacgatgc gagcgcgtgg gcgacgttgt
     5341 tcgcggtcag cccgtccgga taggccgccg cgaagcgccc ggcatagtcg ccctcggcgg
     5401 cgagcttggc gacgagctgc tccgaaccca tgttcatgac cttcgggttg gcgagcggga
     5461 gcagggcgtg cgcggcgagg tcgcaggtgc gtccgtccca gttataacaa tggttgaagg
     5521 cgaggttgaa gatgctcggt gcctggaccg cccccaccat gccaccgctg ccgagcgacc
     5581 gcggccgggg gtcggcgccg ccatgctcga aggggtgaca atcgatgcag cgcgtcttgc
     5641 cgtcctgtga cagcgccggg tcgacaaaca ggcgccgtcc caaagcgacc tgccgctcgt
     5701 cgcccgcgag ctcggacgcc ggtgggatcg gccggatcgg ctcgccgggc cgggccgcga
     5761 cgagcaggtg cggagggagc aacgacggca tccggagctc gggcgcgtcc gtgcaaccgg
     5821 cagcaaaggt gagccacgat cccagggcac gggcggccat ggcatgaccc gaagcccgcg
     5881 gcattcgtta ctttccaccc gcgcgctcgg ccggcgccat cggctcgtcc gcggggtcca
     5941 taccgcattc tacttcagag tcgcagcgct gggtgccaaa gcgcagacat tcgtcccgac
     6001 gcgcgtggag gacgtccaca cctcgacatt acgattatcg aggccctgga tcccgcgctg
     6061 gtgcgagaat cgacgtttta gggccagata tcgcgtaccc atggcgcgct acggcgtcgt
     6121 gggcgtggcc tgcgtcatgt cgaccgcctc ggacggcgcc gtgaggataa acggacgcgc
     6181 ggggggctcg atccatcgga ccggccccac gaccgtcgcg ccgcggtccg gggttcgttc
     6241 gcgcagcagc gtgacgaatc gatcgctgcg catcagcgct ttgtcgccag ctcctcgagg
     6301 tgctccgcca ctccggggcg tcgcgggtcg agctcgcggt agctcgacca ggcgcgctcg
     6361 gcagccgcga ggtcgccgcg acgctcgtgg gcgatcgcca ggatctcgag caggctgagc
     6421 cgggcggccc cggtctgttt ggcgagcgct gcctcgcagg cggcgagcac cggcggatgc
     6481 tcggccgcgt tgctggcgag tgaccacatc gcggacgcct tcgtggccgc ggccacggcg
     6541 acctcgggag gtaagggcgc cggcaatgcg tcgacgaagc tcttgaaccg ggggccgtcg
     6601 agccggccgt cgctcatgat cgcgcgcagg tcgagatcgt cgaggttttg cggcgcatgc
     6661 gtccgtgggt cgaagcgaaa gcccagcggg gcgagggccc gcagggtcgc cgcctgggcg
     6721 tccacgggcg acgcggcgag cagcgcttgc aggtgatcgc ggacgcgatc tggccacgcc
     6781 aggaccgcga ggatgtcccc gagatggacg gtgtcgccgg tcgccatgaa ctggatccac
     6841 cgcacgtcga gctgaccccc gccggtgatc ggtgcgcggg tcgcagcttc gatctccggg
     6901 gcaggcccga ccccaccacg cagcgtctcg gcgatgagcg ccgcggccgg ctggcccttc
     6961 gacagctcgg ccgagcttcg caggaagcgc tcggtctctg catcgccggc gtgggcgagt
     7021 aacttgatga cgatccccac ctgctcgcca ggcaatgctc gcgccacatc ttcgtaggcc
     7081 cggagcacct ccggccggcc ccgcgccgcg gccgtgaaga acacgactcg ctggaggatc
     7141 cgctctgccg ccagctcgac tcgttcggcg tctcccgagt ccgccccggc catcagcgcg
     7201 gggaattcat cgcggacgaa cgcctccaac atcgcgcgtg cgtgcgccgg tgtgccgtcg
     7261 cgataaaaaa agttgtggcg caggcgctcc ggctcgccgc ccggagtgac gacataacga
     7321 tcgggatcca cggcgccctg cacgagctcg gggctcgctg gcccaggtgt catggtgtct
     7381 cgtggcgccg attcgccggg cgtcgggcgc gagggcgctg gagcgacctg cgcagcccgc
     7441 tcctccgtcg cgcaggcgag gctctgtgcg agcacggtga tcgacaaggt gcgccatcga
     7501 tgcataccgt ccagcatccg cgcttttcgt cgtgcacgca agtacacgcg ccagccggag
     7561 cgctgcgata tttgatcgta agggtgcatg gggttcccca aagcgccgcg atccgataca
     7621 tgcgcgggcg tgccccgcga gcctgcccgt cccgccgcgc cgcgcttcac ggcgcaggcc
     7681 ctgaccgccg acctcgatcc acgtatctgt atcgtcgatg tcgcgttggc ggagaagctg
     7741 cagacgcggc acacgcagac ctttgccatc cgccgcgagc ctggtgacga gcgcgacgtc
     7801 ccgaacgtcg tttgaggagg acctggcgca gctcgacgac cgcggcctgg cgatgatcga
     7861 cagccctgtc gggccgggcg cgatcctcgt cggcaagtcc tcgcccgtcg agggtggctc
     7921 gctgtcgccg gaggagctgc tgcgggcgat cttcggcgat gccgcgggcc ctgtgcgcga
     7981 cacctcgctg cgcgcgccgc cgtggtgcac cggcgaggtc agcctggccc gcgccggcat
     8041 caccacgacc cgcgagcgca cgtcacggcg ggcagatgag cgcggcgacg atgtcgcccg
     8101 gctcgccctc ctgcgcgaat tgccccgcgc tgtcgccggc gatcacgggc gtgccgtcgc
     8161 tcagcacggc gacggaggcc ccgctctcat ccttttttgt gccgaactga cgcgtccact
     8221 gcttggttcc actggccgcg tacctggtca cgaagaagtc gctgccgccc accgcggcgt
     8281 tgccgtccag cggaccgaag gccgagccgc cgatcaggac ggcgccggcc tcgtcgacga
     8341 cgacgctgag gccaggcggg gcgcccggct ccgggtcggg aggggcggcg gtcgtcgcct
     8401 ggcctgatca tcactgctga aaacagtaga ggtgcgccgt gagatccttg cacgactggg
     8461 cgctgccgct cctggtccag ccatcggtgg tgacgtcgcg caggccgtag aagcccatgt
     8521 ttccctgggc cgaccagacc gagcagtccg acgggctcca gttggtcccg ttgatgttgg
     8581 tcgcc
//
LOCUS       DASWNJ010001172         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_160514, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001172 DASWNJ010000000
VERSION     DASWNJ010001172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160514"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VGB85_19745"
     CDS             complement(<1..792)
                     /locus_tag="VGB85_19745"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0136333.1"
                     /translation="MRVQLGSWRATIVACEPRVRPVPGRLGMIGGSPAFYALERSLLR
                     LAGLPQAVLVRGETGTGKELAARALHTQGPRVAGPFVPLNCGAIPEGLFESELFGHVK
                     GAFTGATRVHTGAFVRAHGGTLFLDEIAELPLPLQAKLLRVLETRRVSPVGGEAEVPI
                     DVRVVAATHQPLEALVAAGRFRGDLYHRLSALEIDLPALRQRRADIPPLLEHFAAELA
                     IELGRPVVLTPSAVTAATAHDWPGNIRELRNAVLRAAAGCDGPLTA"
     gene            complement(708..1118)
                     /locus_tag="VGB85_19750"
     CDS             complement(708..1118)
                     /locus_tag="VGB85_19750"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0136334.1"
                     /translation="MHGTPTPPILEPTATPLRDREATIHLGAATLAVRLTLLHDDGSV
                     ALDPTRPLLLGTAAPADVRLTDRYASARHARIVAEGKRLVIEDLESTNGTWVDGVRVQ
                     RAYLAPACACSSARGAPRSSPASRACAPSPAASA"
     gene            complement(1181..1408)
                     /locus_tag="VGB85_19755"
     CDS             complement(1181..1408)
                     /locus_tag="VGB85_19755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136335.1"
                     /translation="MATTIYAWDFFGPHAEGTAQHFLRHLHEFWRQSGVEGCEAGLES
                     AGTGHHAVWCKTPEAADAAIQKLRPRRRAPA"
     gene            1477..2181
                     /locus_tag="VGB85_19760"
     CDS             1477..2181
                     /locus_tag="VGB85_19760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136336.1"
                     /translation="MLLIRRFMGFNGFNVVVAGLMTMAACGDSSGGTASDSNGVSQSG
                     TGGVTEPTGGDTEPTGGASMSGTTGAEGGMSDSLSGTTGAEGGMSDSLSGTVSSGVES
                     DSMSGSGSSATMTTGMTGTSVGTEGTEGTEDGGDETSTGDPVDCASFVDEVLCKAAGC
                     MAIVGRRFVSDDAMFCLDPSSFLACTEEAACAGVITTACKGQVKYQLPNACIPDNFVQ
                     CDPPADPDMDGYVDCQ"
BASE COUNT          333 a          782 c          939 g          297 t
ORIGIN      
        1 cgcggtcagc gggccatcgc agccggcggc ggcgcgcagg acggcattgc gtagctcgcg
       61 gatgttgccg ggccagtcgt gcgccgtcgc ggccgtgacc gcggagggcg tcaacacgac
      121 cggacggccg agctcgatcg ccagctcggc ggcgaagtgc tcgagcagcg gcgggatgtc
      181 ggcccgccgc tggcgcaggg ccggcaggtc gatctcgagg gcgctcagac ggtggtagag
      241 gtcgccgcgg aagcgaccgg cggcgaccag cgcctcgagc ggctggtggg tcgccgcgac
      301 gacgcggacg tcgatcggga cctcggcctc gccgccgacc gggctgacgc gccgcgtctc
      361 gagcacgcgc agcagcttgg cctgcagggg cagcggcagc tcggcgatct cgtcgaggaa
      421 cagggtgcct ccgtgggccc ggacgaacgc gccggtgtgc acgcgggtcg ccccggtgaa
      481 ggcgcccttg acgtgcccga acagctcgct ctcgaacagg ccctcgggga tcgccccaca
      541 gttgagcggc acgaacgggc cggcgacccg cgggccctgg gtgtgcagcg cccgcgcggc
      601 gagctccttg ccggtgccgg tctcgccgcg cacgagcacg gcctgcggca agccggcgag
      661 gcgcagcagc gaccgctcta gcgcatagaa cgcgggcgat ccgccgatca tgccgaggcg
      721 gccggggacg gggcgcacgc gcggctcgca ggcgacgatc gtggcgcgcc acgagccgag
      781 ctgcacgcgc acgccggggc caggtaggcc cgttgcacgc gcacgccgtc gacccaggtg
      841 ccgttggtcg actcgaggtc ctcgatcacg aggcgcttgc cctcggcgac gatgcgggcg
      901 tgccgggccg aggcgtagcg gtcggtgagc cgcacgtcgg cgggggcggc ggtgccgagc
      961 agcagcggcc tcgtggggtc gagcgcgacg ctgccgtcgt cgtggaggag ggtcagccgg
     1021 acggccagcg tcgcggcgcc gaggtggatc gtcgcctcgc ggtcacgcag gggcgtcgcg
     1081 gtgggctcga ggatgggggg agtgggcgtt ccgtgcacgc tcactgttcg acctcgcgcg
     1141 cgccgctgtt gcgcgccgat caccgacgaa acgagccggc tcaggcgggg gcgcggcgcc
     1201 gcgggcggag cttctggatc gcggcgtcgg cggcctcggg tgtcttgcac cacaccgcgt
     1261 ggtgcccggt ccccgccgac tcgaggccgg cctcgcaacc ctcgaccccc gactggcgcc
     1321 agaactcgtg cagatgtcgc aggaagtgct gcgcggtgcc ctccgcgtgc gggccgaaga
     1381 agtcccaggc gtagatcgtg gtcgccatcg ccgcgggtac tgtcggcgcg aacgcccggt
     1441 ttgacaagta atacgcgagg ctcctatgct cgtcatatgc tccttattcg gcggtttatg
     1501 ggcttcaacg gcttcaatgt ggtggtggcc gggctgatga cgatggccgc ctgcggcgac
     1561 agcagcggcg gcacggcctc ggattcgaac ggcgtgagcc agagcggcac cggcggggtg
     1621 accgagccga cgggcggcga caccgagccg acgggcggcg cctcgatgag cggcacgacg
     1681 ggagccgagg gcgggatgag cgacagcctc agcggcacga ccggggccga gggcgggatg
     1741 agcgacagcc tcagcggcac ggtcagctcc ggggtcgagt cggacagcat gtcgggctcg
     1801 gggtcgtcgg cgacgatgac gacggggatg acgggtacga gcgtcgggac cgagggcacc
     1861 gagggcaccg aggacggcgg ggacgagacc agcaccggcg atccggtcga ctgcgcgagc
     1921 ttcgtcgacg aggtgctgtg caaggcggcc ggctgcatgg cgatcgtcgg gcgtcggttc
     1981 gttagcgacg acgcgatgtt ctgcctcgac ccgtcgagct tcctggcgtg caccgaggag
     2041 gccgcgtgcg ccggggtgat cacgaccgcc tgcaaggggc aggtcaagta tcagctgccc
     2101 aacgcctgca tcccggacaa cttcgtgcag tgcgacccgc ccgcggaccc ggacatggac
     2161 ggctacgtcg actgccagtg agcgtcagcc ggcgggcggc gtgcggtcgt cggtcgaccg
     2221 cacgacgagc acgctgcacg tcgcgtggtt gacgacgcgg gcggcggtgg tgccgagcac
     2281 acgatcgagg acgccgaagc cgtggctgcc gatcacgatc aggtcgaccg ccagctcggc
     2341 ggcgacgtcg g
//
LOCUS       DASWNJ010001173         6499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_160523, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001173 DASWNJ010000000
VERSION     DASWNJ010001173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160523"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1807
                     /locus_tag="VGB85_19765"
     CDS             <1..1807
                     /locus_tag="VGB85_19765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136337.1"
                     /translation="SGRLAEAAEHTRVLADMSEDPVERGRTLLRAGMLFHEAAGVAPE
                     DGDEDTGPGVALRASAFECVRIGISLIADRPAPVLDRSQLEAAFWTAAPRNAGIALAC
                     LDRLLLNPDVKAVTRQALLIEGARIARERGEPDDEGRALELARAALETLPEAASGAHA
                     VFDVLAMQERISELETLVLGFFDRVGRGRGPNPGETRDRQRLLTRLAVLEVDNPDLAI
                     RLLEQAAELDPQALELESRRRLAHLYEAAAIEGPPVHGNDEALISLDPLDERGLASVA
                     RRCIEAGEKDRAHALYQVLRLVNPGHPEAVAFLGKNDLTQIGNGKLDPALIADKPPPG
                     GGVIAAMTQLWEGAAELICDELPKLEASSAAWIEADSDRDTLLWKVWTELGLTMSTQG
                     VRLADAAMIPGVEVGDGWTQVRAAHPPIVVVGEHARGADTAPRLRFVLGRALFCSRPA
                     AAPIVGLPPHLAAAVVSAALQAFHPRHTRRTRVREDADLAARLAQSFARKLPIRLARQ
                     LSALFKEHEQESFDTRDWRAWAHRGGQRVGLCLARNLGVALDILGLATEPGERSNALK
                     ARVAEDPDLHDLVVFATSPAYVAARKALGFEVRTR"
     gene            complement(2042..2767)
                     /locus_tag="VGB85_19770"
     CDS             complement(2042..2767)
                     /locus_tag="VGB85_19770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136338.1"
                     /translation="MTCEFIARRLILSGLLVAACGGDPGGSTDGGSTGTGTSATTSGT
                     TGEPTTTGVTTGPTSSGESGSGTQGQTDTGATSTGDASTGATGTGSTGSTGSTGEPGS
                     STGEPGSSSGGADLCADFVPPGCMESGCPEGQECKVLADECVSSDCTCDAGTGEIICS
                     PDCGGGSCVPSSCECAADNDCVKTSAGCCPCNAGGQEVPAHKDCVDEVMQCDIPPDQV
                     NCPQVYLCTDAQPACVNGQCVLL"
     gene            complement(2830..3078)
                     /locus_tag="VGB85_19775"
     CDS             complement(2830..3078)
                     /locus_tag="VGB85_19775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136339.1"
                     /translation="MPIQTGEDGVPRALSRFHDLTHVRQTERERFTAQAVRLRGVRPL
                     LTGIQPVVAAALVALAVDFGSLEVFSSLQDAVERATRG"
     gene            complement(3146..4612)
                     /locus_tag="VGB85_19780"
     CDS             complement(3146..4612)
                     /locus_tag="VGB85_19780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY0136340.1"
                     /translation="MRTERTRGAAALGVLAAAVYLWTAPGRIQFPDDEIVFQTAASLW
                     DDGDLDIPGIARRTGEPADRPTGTFGWAPGPDGQRFGFFGHGLSLVAVPAYAIASATA
                     EHVPPAWPHAMRSDHFVFHRRDHRGDWTRLVVSLTNCLVTAVTASVLVGWLRRLGFAA
                     RTALWTGLAYAFATSAWAYSRTFLSEPLSALCLVAAAYCVTRARTDGRRWLWLAGAIA
                     GLSLHVHVLNLVFVPCLIGYVLGDRSWRGWPGAALAFMVGAGLLLIGQQLRFGDPLET
                     GRFGHYSEFVAPWEGLLAQVVAPGRSFFVYSPAVLLGLCGWAALRRRAPAALWFAVAA
                     VVLRWAVISTRSDWFGGWGLGPRHLVPVIPVAMLGFAAALERMPAWRPALRRAWWAAL
                     GLATTLSGWLAAHSIFEWMWKLMSDPRVGDRGLMHVSHWAPWASPIAGFAELRPDVLA
                     LGSILLASLGHPGLLLGFVVIAVVGAVAGWRVVSALRS"
     gene            complement(4658..5167)
                     /locus_tag="VGB85_19785"
     CDS             complement(4658..5167)
                     /locus_tag="VGB85_19785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136341.1"
                     /translation="MSFLRALGVTAIVTMTASLVTACGDDGTSASGFSNGTEGSASSA
                     SSGSSGSSGGVAPTTTETTEAPTGGTGTASGAESSTSTSAPGTTSTTGASDTTTEEGT
                     TAGSSSTTSPAACGNGIIDGKEQCDGADLNGFDCVALGNAGGTLLCDSMTCTFDTQMC
                     DAGGGGTTG"
     gene            5426..>6499
                     /locus_tag="VGB85_19790"
     CDS             5426..>6499
                     /locus_tag="VGB85_19790"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY0136342.1"
                     /translation="MSATTPAVVPPAGAPSWQDSLQRVCAAVVSVRIDATRPFDTEWN
                     GTSQATAFVVDATRGILLTNRHVVHPGPVVAEAVFLNHEEISLRPLYRDPVHDFGFFQ
                     YDPAALRFIAPTSLRLAPEKARVGAEIRVVGNDAGEKLSILAGTLARLDRPAPSYGVH
                     RYNDFNTFYIQAASGTSGGSSGSPVVDIDGDVVALNAGSRTQAASSFFLPLDRVVRAF
                     ERVRAGLPVPRGTLTTTFAYTPYDELRRLGLSPASEQASRDHDPAATGLLVVEKILPR
                     GVTDGLLEPGDILLRANDVPLADFVGLSALLDDHVGEAVRIDIERGGEAMRIDPVVQD
                     LHAITPASFLEVGGGILHDLSYQR"
BASE COUNT          888 a         2665 c         2085 g          861 t
ORIGIN      
        1 cagcggtcgg ctcgccgagg ccgccgagca cacgcgtgtc ctcgcggaca tgtccgaaga
       61 tccagtcgag cgcggccgga ccctgctgcg cgccggcatg ctgttccacg aggccgccgg
      121 cgtcgcgccc gaggacggcg acgaggacac cggcccgggc gtcgccctgc gcgcgtcggc
      181 cttcgagtgc gtgcggatcg gcatctcgct gatcgccgac cgccccgccc cggtgctcga
      241 ccgcagccag ctcgaggccg cgttctggac cgccgcgccg cgcaacgccg gcatcgcgct
      301 cgcctgcctc gaccgcctgc tgctcaaccc cgacgtcaag gccgtcaccc gccaggccct
      361 gctgatcgaa ggcgcacgga tcgcacgcga gcgtggcgag cccgacgacg agggccgcgc
      421 cctcgagctc gcgcgcgccg ccctcgagac cttgcccgag gccgcctccg gcgcgcacgc
      481 ggtgttcgac gtgctggcga tgcaggagcg gatctccgag ctcgagaccc tcgtgctcgg
      541 cttcttcgac cgcgtcggtc gcggccgcgg ccccaacccc ggcgagacgc gcgaccgcca
      601 gcgcctgctg acccgcctcg ccgtgctcga ggtcgacaac cccgacctgg cgatccgcct
      661 gctcgagcag gccgccgagc tcgacccgca ggccctcgag ctcgagtcgc gccgccgcct
      721 cgcgcacctc tacgaggccg ccgcgatcga gggcccgccg gtgcacggca acgacgaggc
      781 gctgatctcc ctcgacccgc tcgacgagcg cgggctcgcc agcgtcgccc gccgctgcat
      841 cgaggccggc gagaaggacc gcgcccacgc gctctaccag gtgctgcgcc tggtcaaccc
      901 cggccacccg gaggccgtcg ccttcctcgg caagaacgac ctcacgcaga tcggcaacgg
      961 caagctcgac ccggctttga tcgccgacaa gccgcccccc ggcggcggcg tgatcgcggc
     1021 gatgacccag ctgtgggagg gcgccgccga gctgatctgc gacgagctgc cgaagctcga
     1081 ggcctcgtcc gccgcgtgga tcgaggccga cagcgaccgc gacaccctgc tgtggaaggt
     1141 gtggaccgag ctcggactca ccatgtcgac ccagggcgtg cgtctcgccg acgccgcgat
     1201 gatccccggc gtcgaggtcg gcgacggctg gacgcaggtc cgcgccgcgc acccgccgat
     1261 cgtcgtcgtc ggtgagcacg cgcgcggagc cgacaccgct ccgcgcctgc gcttcgtgct
     1321 cggccgcgcc ctgttctgct cgcgccccgc cgccgcgccg atcgtcggcc tgccgccgca
     1381 cctcgccgcc gccgtcgtgt cggccgcgct gcaggccttc cacccccggc acacccgccg
     1441 cacccgcgtg cgcgaggacg ccgacctcgc cgcgcgcctc gcccagagct tcgcccgcaa
     1501 gctcccgatc cgcctcgccc gccagctcag cgcgctgttc aaggagcacg agcaggagag
     1561 cttcgacacc cgcgactggc gggcctgggc ccatcgcggc gggcaacgcg tgggactgtg
     1621 cctcgcgcgc aacctcggcg tcgcgctcga catcctcggc ctggcgaccg agcccggcga
     1681 gcgcagcaac gccctcaagg cccgcgtcgc cgaggacccc gacctgcacg acctcgtcgt
     1741 cttcgccacc agcccggcct acgtcgccgc gcgcaaggcc ctcggcttcg aggtccgcac
     1801 gcgctaaaaa acccagcggc gcggtgccgg aggagtgcga cgacggcaac ctcgacggag
     1861 gacggctgcg acgcgacctg tgcggtcgac cgcagggcgt tcgtgctcgg cccccggcac
     1921 cgcggtcccg tccgggcgcg tcccgtccac acgtccggct cgatcacccc gtgccccctg
     1981 cactccgcga acgaccgccc tcaccggcgc tggcgcgctc accgggcgtt cgtgccttcg
     2041 atcagagcag cacgcactgc ccgttgacgc aggccggctg cgcatcggtg cacaggtaca
     2101 cctgcgggca gttgacctgg tccggcggga tgtcgcactg catcacctcg tcgacacagt
     2161 ccttgtgcgc cgggacctcc tggccacccg cgttgcacgg gcagcagccg gcgctggtct
     2221 tgacgcagtc gttgtcggcc gcgcactcgc acgagctcgg cacacacgac ccgccgccgc
     2281 agtccggcga acagatgatc tcgcccgtac ccgcgtcgca ggtgcagtcc gacgacacgc
     2341 actcgtcggc gagcaccttg cactcctgtc cctcgggaca gccgctctcc atgcaccccg
     2401 gcggcacgaa gtcggcgcac aggtccgcgc caccgctgct cgagcccggc tcccccgtgc
     2461 tcgagcccgg ctcacccgtg ctgcccgtgc tgcccgtgct gcccgtgccc gtcgccccgg
     2521 tgctcgcgtc gccggtgctc gtcgccccgg tgtccgtctg gccctgggtg ccgctgcccg
     2581 actcgccgct gctcgtcggt ccggtcgtca cgcccgtggt cgtcggctcg ccggtcgtcc
     2641 cgctcgtcgt cgcgctcgtg ccggtcccgg tgcttccgcc gtcggtgcta ccgcccggat
     2701 cgccgccgca ggcggccacg agcaggccag agaggatgag acgcctcgca atgaattcac
     2761 aagtcatgcg cgagggctta gcacggaacg cgccgccctc gtcgctgtcg tacgcgcccg
     2821 tcgcccggct cagccgcggg tcgcccgttc gacggcgtcc tgcaagctgc tgaacacctc
     2881 gagcgagccg aagtcgaccg cgagcgcgac cagggccgcg gccaccacgg gctggatgcc
     2941 ggtcagcagc ggtcgcacgc cgcggagccg caccgcctgg gccgtgaagc gctcgcgctc
     3001 cgtctggcgc acgtgggtca ggtcgtggaa ccgcgacagc gcccgcggca ccccgtcctc
     3061 gccggtctgg atcggcacgg tcgtgacatc gaagatgctg cacgtgccgt ctggccacat
     3121 cggcctgctg cagctccccg acttttcatg agcgcagcgc agacacgacc cgccaccccg
     3181 ccactgcccc gacgaccgcg atcaccacga agccgagcag cagccccgga tgtccgaggc
     3241 tcgccagcag gatcgacccg agcgccagca catccggtcg cagctcggcg aagcccgcga
     3301 tcgggctcgc ccacggcgcc cagtgtgaca cgtgcatcaa tccgcgatcc ccgacgcggg
     3361 ggtcgctcat cagcttccac atccactcga agatcgagtg cgccgccagc cagcccgaga
     3421 gcgtcgtcgc gaggccgagc gccgcccacc acgcgcgccg cagcgctggg cgccacgccg
     3481 gcattcgctc gagcgccgcc gcgaagccga gcatcgccac cgggatcacc ggcacgaggt
     3541 ggcgcggccc gagcccccag ccgccgaacc agtcgctgcg cgtcgagatc accgcccagc
     3601 gcagcacgac cgccgcgacc gcgaaccaca acgccgccgg cgcccgccgg cgcagcgccg
     3661 cccacccgca cagcccgagc agcaccgccg gcgagtacac gaagaagctg cgccccggcg
     3721 cgaccacctg cgccagcagg ccctcccacg gggccacgaa ttcgctgtag tggccaaagc
     3781 gcccggtctc gagcgggtcg ccgaagcgca gttgctgccc gatcagcagc agccccgcgc
     3841 cgaccatgaa cgcgagcgcc gcgcccggcc agccgcgcca tgacctgtcc cccagcacat
     3901 agccgatgag acatggcaca aagaccaggt tgaggacgtg cacgtgcagc gagagccctg
     3961 cgatcgcgcc ggcgagccac agccagcgcc ggccgtccgt gcgcgcccgc gtgacgcagt
     4021 acgccgccgc gaccaggcac agcgcgctca gcggctcgct gaggaacgtc cgcgagtacg
     4081 cccacgccga ggtcgcgaac gcgtacgcca acccggtcca cagggccgta cgcgccgcga
     4141 agccgagccg ccgcagccac ccgacgagca ccgacgccgt gaccgccgtg accaggcagt
     4201 tcgtcaggct gacgaccagc cgggtccagt cgccgcggtg gtcgcggcga tgaaacacga
     4261 agtggtcgga ccgcatcgcg tgcggccagg ccgggggcac gtgctccgcc gtcgcgctcg
     4321 cgatcgcgta tgccggcacg gcgacgagcg acagcccgtg gccgaagaat ccgaagcgct
     4381 gaccgtccgg gccgggcgcc cagccgaagg tcccggtcgg gcggtcggcg ggctcgccgg
     4441 tgcgtcgcgc gatgccgggg atgtcgaggt caccgtcgtc ccacaggctc gcggccgtct
     4501 ggaacacgat ctcgtcgtcg gggaactgga tccggccggg cgcggtccac agatagaccg
     4561 ccgccgcgag cacccccagc gccgccgctc cgcgcgtgcg ctccgtcctc atgtcctcgc
     4621 gagcccacgc atggggcgtc actcgtgtgc cgcgacctca gccggtcgta ccgccaccgc
     4681 cggcgtcgca catctgcgtg tcgaaggtgc aggtcatcga atcacacagc aaagtgccgc
     4741 cagcgttgcc aagcgccacg cagtcgaagc cgttgaggtc cgccccgtcg cactgctcct
     4801 tgccgtcgat gatgccgttg ccgcaggccg ccgggctcgt cgtgctgctg ctgccggccg
     4861 tcgtgccctc ctccgtggtc gtgtcggagg cgcccgtcgt gctcgtcgtg cccggagcgc
     4921 tcgtgctcgt cgacgactcc gcgcccgacg ccgtgccagt gccgcccgtc ggcgcctcgg
     4981 tcgtctcggt cgtcgtcggc gcgacgccgc cgctgctccc gctgctgccg ctgctcgcac
     5041 tgctggcgct gccctcggtg ccgttcgaga agccgctcgc ggacgtcccg tcatcaccgc
     5101 acgccgtcac cagcgaggcc gtcatcgtga cgattgcagt tacaccgagg gcgcgcaaaa
     5161 agctcatgtc gccgttttcc tcccggcggc cccagcggtc aagcaaaaag cccggaccgc
     5221 ccgcgacccc cacgcccgcc ggtcatgagc gcggtcacga cctcgctcga ccgacctcgc
     5281 tcgaccgacc tccgctctac cctcgctcta cctccgcctc gtccggggac cgtcgcctga
     5341 cgcccccgct tgcttccgct tgcttgcgct tgcttccgca tcgcggtctt gattaccggc
     5401 gcgtccacag ccacgttagc aggccgtgag cgcgacgacc cccgctgtag tcccccccgc
     5461 tggcgcgccg agctggcaag acagcctgca acgggtgtgc gccgcggtcg tctcggtgcg
     5521 catcgacgcc acccgcccgt tcgacaccga gtggaacggc accagccagg caacggcgtt
     5581 cgtcgtcgac gcgacgcggg gcatcctgct gaccaaccgt cacgtcgttc accccggccc
     5641 cgtcgtcgcc gaggccgtct tcctcaatca tgaggagatc tcgctgcggc cgctctatcg
     5701 cgaccccgtc cacgacttcg gcttctttca atacgacccg gccgcgctgc gcttcatcgc
     5761 cccgacctcg ctgcgcctcg cccccgagaa ggcccgcgtc ggcgccgaga tccgcgtcgt
     5821 cggcaacgac gccggcgaga agctctcgat cctcgccggc accctcgccc gcctcgaccg
     5881 ccccgccccc tcctacggcg tccatcgcta caacgacttc aacaccttct acatccaggc
     5941 cgcgtcgggc acctccggcg gctcctccgg ctctcccgtc gtcgacatcg acggcgacgt
     6001 cgtcgccctc aacgccggct cgcgcaccca ggccgcctcg agcttcttcc tcccgctcga
     6061 ccgggtcgtc cgcgccttcg aacgcgtgcg cgccgggctc cccgtcccgc gcggcaccct
     6121 gaccacgacc ttcgcgtaca ccccctacga cgagctgcgc cgcctcggcc tcagccccgc
     6181 cagcgaacaa gcttcgcgtg accacgaccc cgccgccacc ggcctgctcg tcgtcgagaa
     6241 gatcctcccg cgcggcgtca ccgatggcct gctcgagccc ggcgacattt tactgcgcgc
     6301 gaacgacgtc ccgctcgccg acttcgtcgg cctctcggcg ttgttggacg accacgtcgg
     6361 cgaggccgtc cgcatcgaca tcgagcgcgg cggcgaggcc atgcggatcg accccgtcgt
     6421 ccaggacctg cacgcgatca cccccgcgag cttcctcgag gtcggcggcg ggatcttgca
     6481 cgacctctcg tatcagcgc
//
LOCUS       DASWNJ010001174         4294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_22141, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001174 DASWNJ010000000
VERSION     DASWNJ010001174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22141"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VGB85_19795"
     CDS             <1..465
                     /locus_tag="VGB85_19795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136343.1"
                     /translation="PEGDAGRLWHIVPRLDGIREHLALRPAQARPRLLAALATAVTAA
                     LRLWLRHGVALELDLGSFAVEAGRVVHIGDRIGTQQRDVGVALLGLCERLSGDAAALA
                     AFTAALEQELAELAPEERARLELEAALVRAETRYQDAHVARNRLLAALRAPG"
     gene            complement(505..807)
                     /locus_tag="VGB85_19800"
     CDS             complement(505..807)
                     /locus_tag="VGB85_19800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEY0136344.1"
                     /translation="MSLESYTETVRSKVGENANLNSTIKFAFKEGGALYIDGKSTPNT
                     VSNDENAPADCTVKVSLEDFGKLMERKLDPTTAFMMGKIKIEGDMGVAMKLGRLFG"
     gene            1011..1883
                     /locus_tag="VGB85_19805"
     CDS             1011..1883
                     /locus_tag="VGB85_19805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0136345.1"
                     /translation="MGAYRSVFRDGLFAGRTIVVTGGGSGIGRCTAHELAALGAHAVL
                     VGRKPERLAAVAAEITADRGDPGTVSFHACDIRDEAAVKATIATILTDRGKIDGLVNN
                     AGGQFPAPLSAISQKGFETVVRTNLVGGFLVAREVYTQWMAAHGGAIVNIVADMWRSM
                     PGMGHSGAARAGMVNFTQTAAVEWGSSGVRVNAVAPGWIASSGLDHYEGAAKAMIPRL
                     RGAVPLGRLGTESECSAAICFLLSEAAAFISGTTLRVDGAAPNATSLWPLEAHDNAPA
                     YDGFHRATLPEVLR"
     gene            2779..>4294
                     /locus_tag="VGB85_19810"
     CDS             2779..>4294
                     /locus_tag="VGB85_19810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY0136346.1"
                     /translation="MPVLVSKIDTQSDAFKANRDAQLALISEFRAAEQRVRDNSNRQA
                     EKFSKRGQLLPRERVARLLDRGAPFIELATLAGHKMHDDDGRSDAAGGGNISGIGFVS
                     GVRCIIGASDSAIKGGSIAPMGLKKSLRTQEIALQNKLPVVSLIESAGANLLYQSEIF
                     VDGGRVFANMARLSAAGLPQITVVHGSSTAGGAYMPGLSDYVIAVRGAAKVFLAGPPL
                     VKAAIGEDADDEQLGGAELHATVTGTAEYMAETDAHAIHMAREIMANLAKAPWRNLHG
                     ETTTRPHAPPRHDIEELLGVVPADFKKQYDAREVIARIVDGSDFLEFKHLYGRATVCG
                     HAALEGHACAIIANNGPIDPDGATKAGQFIQLCCQSGTPIIYLQNTTGYMVGSAAERA
                     GMVKHGSKMIQAVANATVPQITLVIGGSFGAGNYGMCGRGFDPRFVFAWPASRVAVMG
                     GEQAAKVMEIITLAKFARMGVQGDADAIATMGKSIRDKLDAESHALYATARLWDD"
BASE COUNT          614 a         1694 c         1381 g          605 t
ORIGIN      
        1 ccggagggcg atgcgggccg cctgtggcac atcgtcccgc gcctcgacgg catccgcgag
       61 cacctcgcgc tgcgccccgc ccaggcccgc ccgcgcctgc tcgccgcgct cgctaccgcc
      121 gtcaccgcgg cgctgcgcct gtggttgcgc cacggcgtcg cgctcgagct cgacctcggg
      181 tccttcgccg tcgaggccgg ccgcgtcgtc cacatcggcg accggatcgg cacgcagcag
      241 cgcgacgtcg gcgtcgccct gctcggcctg tgcgagcgcc tcagcggcga cgcggccgcg
      301 ctcgcggcct tcaccgccgc gctcgagcag gagctcgccg agctcgcccc cgaggagcgc
      361 gcccgcctcg aattggaggc cgccctggtg cgcgccgaga cgcgctacca ggacgcccac
      421 gtcgcccgca accgcctcct cgcggccctg cgcgcgcccg gttgacttca cggccctccg
      481 ggcctgatcg cccccgaccg ccgatcagcc gaacaggcgg ccgagcttca tggcgacgcc
      541 catgtcgccc tcgatcttga tcttgcccat catgaacgcc gtcgtcgggt cgagcttgcg
      601 ctccatcagc ttgccgaagt cctcgaggct gaccttgacg gtgcagtccg cgggggcgtt
      661 ctcgtcgttg ctgaccgtgt tcggcgtgct cttgccgtcg atgtagagcg cgccgccctc
      721 cttgaaggcg aacttgatcg tggagttgag gttggcgttc tcgccgacct tgctgcggac
      781 cgtctccgtg taactctcga ggctcatggc tgctgtccct ttccccgcga acgggtggat
      841 cttcgatagc ctcgcgtgcg taccacaccc ggacccacgg acgcgacccg gtgaaacgcc
      901 cggcgcacac cgctaaaagc cggccgcggt cccccggacg tcgcgcgggc gcccgccccc
      961 ggccgcttcc ctgctgtgcc ggccccgccg tgcgcgtaag atgcaccgcc atgggcgcct
     1021 accgttccgt gtttcgtgac ggcctcttcg ccggtcggac catcgtcgtc accggcggcg
     1081 gctccggcat cggccggtgc acggcgcacg agctggcggc cctcggcgcg cacgcggtgc
     1141 tcgtcggtcg taaacccgag cgcctcgccg ccgtcgccgc ggagatcacc gccgaccgcg
     1201 gtgaccccgg caccgtcagc ttccacgcct gcgacatccg cgacgaagcc gccgtcaagg
     1261 cgacgatcgc cacgatcctt actgaccgag gcaagatcga cggcctcgtc aacaacgccg
     1321 gcggccagtt cccggccccg ctctcggcga tctcgcagaa gggcttcgag acggtcgtgc
     1381 gcaccaacct cgtcggcggc ttcctcgtcg cccgcgaggt gtacacgcaa tggatggccg
     1441 cgcacggcgg cgcgatcgtc aacatcgtcg ccgacatgtg gcgcagcatg cccggcatgg
     1501 gccactccgg ggccgcgcgc gccggcatgg tcaacttcac gcagacggcc gcggtcgagt
     1561 gggggtcgtc cggcgtgcgc gtcaacgccg tcgcgccggg atggatcgcg tcgagcggcc
     1621 tcgatcatta cgagggcgcc gccaaggcga tgatcccccg cctgcgcggg gccgtgccgc
     1681 tcggccgcct cggcaccgaa tccgagtgca gcgccgcgat ctgcttcctc ctgtccgagg
     1741 ccgccgcctt catcagcggc accaccctgc gggtcgacgg cgccgccccg aacgccacca
     1801 gcctgtggcc gctcgaggcc cacgacaacg cacccgccta cgacggcttc catcgcgcca
     1861 ccctccccga ggtcctgcgt tgagctgcac cgcccgcacc cgccccggct cgccctcgcg
     1921 tcgcgcgggc gccgggtcgc gttgcgcggg cggctcgcgt tgcgcgggcg actcgcgttg
     1981 cgcgggcgcc cgctcgcgtc gggtggacac cgcggggcga ggctacatgg gccgacgggc
     2041 gggggccgac aggcagcggg gccctgcgac gcgggtcctc ccagggcacg tgctcgccgc
     2101 cccacctgtc cggaaggaca tgtctctttc accatcactc tctcgccgct ctcgactggc
     2161 ggtggggcgt ctgcgcgtga gccctgggtc ctcccgctgc ttcccccgcc agtcggcccc
     2221 tgcccgccgg accaacaccg tcggcgcccc gctcgttgca cacgaccgct gtctcgcgcg
     2281 ctcgcggatc ttcgttgccc ggagcggcga catccgggcg cccgatagca cccgaactgt
     2341 agatagcggc gtcgcaacga tccgcgacat cgtcccactc gcgcgctgca tcgacggcat
     2401 ccggactccc cacaatctcc gtgccgcata catcgagagc gtccggcgac tccaccgatc
     2461 gccggcgacc gacagcgggg cgccgacggt gcccgcccgg ggggcagggg ccgacggcgg
     2521 gggaagcagc gggaggaccc agggctcgcg cgcagacgcc ccctcgtccc tgcggtcacg
     2581 gtatacaggc tcgatgaaac ggccatgtct ttcgggacag atggggcggc gagcacgtgc
     2641 tctgggagga cccgcgtcgc agggacccgc agtccgacgg cccccgcccc ccggcccaca
     2701 cagcctcgcc ccgcgctccc cgaacgcgcc cacaccgagc ccgcgaaccc cgaacccgcc
     2761 ccagacagcg ctgtagccat gcccgtcctc gtctccaaga tcgacacgca gtccgacgcc
     2821 ttcaaggcca accgtgacgc ccagctcgcg ctgatctccg agtttcgcgc ggccgagcag
     2881 cgggtccgcg acaactcgaa tcgccaggcc gagaaatttt ccaagcgcgg ccagctgctc
     2941 ccgcgcgagc gcgtcgctcg tctgctcgac cgcggcgcgc ccttcatcga gctggcgacc
     3001 ctcgccggcc acaagatgca cgacgacgac ggccgctccg acgccgcggg tggcggcaac
     3061 atcagcggca tcggcttcgt ctccggcgtg cgctgcatca tcggtgccag cgacagcgcg
     3121 atcaaaggcg gatcgatcgc tccgatgggt ctaaaaaaat ctctgcgcac ccaggagatc
     3181 gcgctgcaaa acaagctccc ggtcgtctcg ctgatcgaga gcgccggcgc caacctcctc
     3241 taccagtccg agatcttcgt cgacggcggc cgcgtgttcg ccaacatggc gcgcctgtcc
     3301 gccgccggcc tgccacagat caccgtcgtg cacggctcct cgaccgccgg cggcgcgtac
     3361 atgccgggcc tgtcggacta cgtgatcgcc gtgcgtggcg cggccaaggt gttcctcgcc
     3421 ggcccgccgc tggtcaaggc cgcgatcggc gaggacgccg acgacgagca gctcggcggc
     3481 gccgagctgc acgcgaccgt caccggcacc gccgagtaca tggccgagac cgacgcccac
     3541 gcgatccaca tggcccgcga gatcatggcc aacctcgcga aagccccgtg gcgcaacctc
     3601 cacggcgaga ccaccacgcg cccgcacgcc cccccgcgcc acgacatcga ggagctgctc
     3661 ggcgtcgtcc ctgccgactt taaaaaacaa tacgacgcgc gcgaggtgat cgcccgcatc
     3721 gtcgacggct ccgacttcct cgagttcaag cacctctacg gccgcgccac cgtatgcggc
     3781 cacgcggcgc tcgagggcca cgcctgcgcg atcatcgcga acaacggccc gatcgacccc
     3841 gacggcgcga ccaaggctgg ccagttcatt cagctgtgct gtcaatccgg caccccgatc
     3901 atctatctac aaaacaccac cggttacatg gtcggcagcg cggccgagcg cgccggcatg
     3961 gtcaagcacg ggtcgaagat gatccaggcg gtcgccaacg cgaccgtccc gcagatcacc
     4021 ctcgtcatcg gcggcagctt cggcgccggc aactatggca tgtgcggccg cggcttcgac
     4081 ccgcgcttcg tgttcgcgtg gccggccagc cgcgtcgccg tcatgggcgg cgagcaggcc
     4141 gccaaggtca tggagatcat cacgctcgcc aagttcgccc gcatgggcgt gcagggcgac
     4201 gccgacgcga tcgccaccat gggcaagtcg atccgcgaca agctcgacgc cgagtcccac
     4261 gccctctatg ccaccgcccg cctgtgggac gacg
//
LOCUS       DASWNJ010001175         2397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_160532, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001175 DASWNJ010000000
VERSION     DASWNJ010001175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160532"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..1900)
                     /locus_tag="VGB85_19815"
     CDS             complement(248..1900)
                     /locus_tag="VGB85_19815"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEY0136347.1"
                     /translation="MSTGTPGATTAKAGEVAEIVDLRALGLPRLVFALLRARFSGLLT
                     LPQTEPEPGERTVWFQGGMPIFTDWVSPEDSLGQILVETGQLAAEERDRAAQALDSLP
                     PTAARERFGHYLVRRRLLTTAQLRKALRVQCGRKLVHCFAVRRGEARVTGGESSEVDE
                     HTLGAQVNALELIFAGVTRHYDLGRIAIDMARLFEAPLRVRSSLERYRAHFRFTPADE
                     AVIAAFNPSATILEVAARSSESRLRVAQIAYTLWACQMLKGAQAMRDTSGAQAVVGAK
                     AEGEPRPAARKASIGAGNVDPDRLAEFAAELGRLEAMVTAGGNAFELMGLPLTAGRAE
                     VRAAWHGLSRRFHPDALAHQELGHLRERSAAVFASLNEAYQILSDTRQREELAALLRS
                     GGRPQGHNTPAPTTQAILESEAIAREADKLLRAGNFERALERYRKAAELSPEDLELQV
                     AIAWCEYQRSADKAPLRGPTEVTLRGVLSRQPRCARAHYFLGLLYMHASDDQAALAAF
                     TAALEIEPEMLDAKRQIHAIELRGSTPPATDPRSSGVFRRRI"
     gene            1952..>2397
                     /locus_tag="VGB85_19820"
     CDS             1952..>2397
                     /locus_tag="VGB85_19820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEY0136348.1"
                     /translation="MSISFMVGPHRVRFAGKTDIGRVRAHNEDNILVPKEMALAVVSD
                     GMGGHASGEVASRITVETIDQYYRDTARSGPTTWPLKLPNLEVERQRMAVAIQLANSN
                     IFETAAAEVGKKGMGCTVDAMYFSQGRFYIGHVGDSRVYRIRGSNI"
BASE COUNT          350 a          859 c          854 g          334 t
ORIGIN      
        1 cagcgcgcga aagcacagga cctgttgcag gcgcgccagg tcggcgtcgc tgtacaggcg
       61 atagccgacg gcgctgcgcc ccgatggttt gagcaggccg atctcgtcgt aatgatgcag
      121 cgttcgtacg ctcaccccgg cgatgcccgc gacctgactg actgtgagtg ccatggaagt
      181 ctgcgcctcg acgacaggga tacggcctca cgtcgcgtga gggtcaagcc cgccgcgcaa
      241 gaaaatttca gatacgccgg cgaaacacgc cggaggagcg cgggtcggtc gccggcggcg
      301 tcgagccgcg aagctcgatc gcgtggatct ggcgcttggc gtcgagcatc tccggctcga
      361 tctccagcgc ggccgtgaag gcggccagcg cggcctgatc gtcgctcgca tgcatgtaca
      421 gcaggccgag gaagtagtgc gcccgggcgc agcgcggctg gcgggagagg acaccgcgca
      481 gcgtcacctc ggtcgggccg cgcagcggcg ccttgtcggc gctgcgctgg tactcgcacc
      541 aggcgatcgc aacctgcagc tcgaggtcct ccgggctcag ctcggcggcc ttgcggtagc
      601 gctcgagcgc gcgctcgaag ttgccggcgc gcagcagctt gtcggcctcg cgagcgatcg
      661 cctcgctctc gaggatcgcc tgggtcgtcg gcgccggcgt gttgtgaccc tgcgggcgcc
      721 cgccggagcg cagcagggca gccagctcct cgcgctgtcg cgtgtcgctg aggatctggt
      781 aggcctcgtt gaggctggcg aacaccgccg ccgagcgctc gcgcaagtgg ccgagctcct
      841 ggtgcgccag cgcgtccggg tgaaagcgac ggctcaggcc gtgccacgcc gcgcgcacct
      901 cggcgcgacc ggccgtcagc ggcaggccca tcagctcgaa ggcgttgccg ccggccgtga
      961 ccatcgcctc gagccggccg agctccgccg cgaactcggc gaggcggtcg gggtcgacgt
     1021 tgccggcgcc gatgctcgcc ttgcgcgcgg ccggcctggg ctcgccctcc gccttcgcgc
     1081 cgacgaccgc ctgcgcgccg gaggtgtcgc gcatcgcctg ggcgcccttg agcatctggc
     1141 aggcccacag cgtgtacgcg atctgggcga cgcgcaggcg gctctcactg ctgcgcgccg
     1201 cgacctcgag gatcgtcgcg ctggggttga acgccgcgat cacggcctcg tccgcgggcg
     1261 tgaagcgaaa gtgggcccgg tagcgctcga gcgacgagcg cacgcgcagc ggcgcctcga
     1321 acagccgcgc catgtcgatc gcgatccgcc cgaggtcgta gtgcctggtg acgccggcga
     1381 agatcagctc gagcgcgttg acctgggcgc cgagcgtgtg ctcgtcgacc tcgctcgact
     1441 cgccgccggt cacccgcgcc tcgccgcgac gcaccgcgaa gcagtgcacc agcttgcgac
     1501 cgcactggac ccgcagcgcc ttgcgcagct gggcggtcgt cagcaggcgc cggcggacca
     1561 ggtagtggcc gaagcgctcg cgggcggccg tcggcggcag gctgtcgagc gcctgggcgg
     1621 cgcgatctcg ctcctcggcc gcgagctggc cggtctcgac gaggatctgc ccgagcgagt
     1681 cctccgggct gacccagtcg gtgaagatcg gcatgccgcc ctggaaccac accgtgcgct
     1741 cgccgggctc cggctcggtc tgtggcagcg tcagcagacc gctgaagcgg gcccgcaaaa
     1801 gcgcgaacac gagccgcggc agacccaggg cccggagatc gacgatctcc gcgacctccc
     1861 ccgcctttgc ggtcgttgcg ccaggcgtgc ccgtgctcat ccgcgggaga tgctagcact
     1921 cggcggtgca gccctgctac ggtgcgagcg catgagcatc agcttcatgg tcgggccgca
     1981 ccgcgtccgg ttcgccggca agacggacat cggccgggtc cgggcccaca acgaggacaa
     2041 catcctggtc cccaaggaga tggccctcgc ggtcgtctcc gacggcatgg gcgggcacgc
     2101 cagcggcgag gtcgccagcc gcatcaccgt ggagacgatc gaccagtatt atcgggacac
     2161 ggcgcgctcc ggcccgacga cctggccgct caagctgccc aacctcgagg tcgagcgcca
     2221 gcgcatggcc gtcgccatcc agctcgccaa ctcgaacatc ttcgagaccg ccgcggccga
     2281 ggtcggcaaa aagggcatgg gctgcaccgt cgacgcgatg tacttcagcc agggccggtt
     2341 ctacatcggc cacgtcggcg acagccgcgt ctatcgcatc cgcggcagca acatcca
//
LOCUS       DASWNJ010001176         1644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_657854, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001176 DASWNJ010000000
VERSION     DASWNJ010001176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657854"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VGB85_19825"
     CDS             <1..586
                     /locus_tag="VGB85_19825"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY0136349.1"
                     /translation="PTTAPNAFATGRNPQHAAVCCTEGILELLDERELRGVLGHELAH
                     VGNRDILIASVAASLATVITFMANMAQFAMIFGGRSREGGNVLSSLLLIILGPLAAGV
                     IQMAISRNREYAADVTGAQVTGDPLALASALRKLERGTAARPLVEDASLAPTSSLMIA
                     NPFRGGGIGRMFSTHPPMAERIARLEQMAGYGYR"
     gene            complement(577..1065)
                     /locus_tag="VGB85_19830"
     CDS             complement(577..1065)
                     /locus_tag="VGB85_19830"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEY0136350.1"
                     /translation="MADPSFDVVSKVDRQEVDNALNQTAKEAATRYDFKGTGAEIAWS
                     GESIVIKANSEERCNALLDVFKEKLIKRQVSLKTLDAGEPQQSGKEYRLTATVNEGIS
                     QDNAKKVSKVIRDQGPKGVQAQIQGDQLRVSAKKKDDLQTVIALLKGEDFDFALQFTN
                     YR"
     gene            1115..1196
                     /locus_tag="VGB85_19835"
     tRNA            1115..1196
                     /locus_tag="VGB85_19835"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1149..1151,aa:Tyr,seq:gta)
     gene            1510..1582
                     /locus_tag="VGB85_19840"
     tRNA            1510..1582
                     /locus_tag="VGB85_19840"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1543..1545,aa:Thr,seq:ggt)
BASE COUNT          225 a          600 c          543 g          276 t
ORIGIN      
        1 ccccaccacc gccccgaacg ccttcgccac cggccgcaac ccgcagcacg ccgccgtctg
       61 ctgcaccgag gggatcctcg agctgctcga cgagcgcgag ctgcggggcg tgctcgggca
      121 cgagctggcc cacgtcggca accgcgacat cctcatcgcc tccgtcgccg cgtcgctggc
      181 cacggtcatc accttcatgg ccaacatggc gcagttcgcc atgatcttcg gcgggcgcag
      241 ccgcgagggc ggcaacgtgc tgtccagcct gctgctgatc atcctgggtc cgctggccgc
      301 cggagtgatc cagatggcca tcagccgcaa ccgggagtac gccgccgacg tcaccggcgc
      361 acaggtcacc ggcgatccgc tcgcgctggc cagtgccctg cgcaagctcg agcgcggcac
      421 cgccgcgcgg cccctggtcg aggatgcctc actggccccg accagctcgc tgatgatcgc
      481 gaaccccttc cgcggcggcg gcatcggcag gatgttcagc acccacccgc cgatggccga
      541 gcggatcgcc cgcctcgagc agatggccgg ctacggctac cggtagttgg tgaactgcag
      601 ggcgaagtcg aagtcctcgc ccttcagcag cgcgatcacc gtctgcaggt cgtccttctt
      661 cttggccgac acccgcagct ggtcgccctg gatctgcgcc tgcacgccct tggggccctg
      721 gtcgcggatg accttgctga ccttcttggc gttgtcctgc gagatgccct cgttgacggt
      781 ggccgtcagg cggtactcct tgccggactg ctgcggctcg ccggcgtcga gggtcttgag
      841 cgagacctgc cgcttgatca gcttctcctt gaagacgtcg agcagggcgt tgcagcgctc
      901 ctccgagttc gccttgatca cgatcgactc gccggaccag gcgatctcgg cgccggtgcc
      961 cttgaagtcg tagcgggtgg ccgcctcctt ggcggtctgg ttcagggcgt tgtcgacctc
     1021 ctggcggtcg accttgctca cgacgtcgaa cgacgggtca gccacgctgc gctccttgct
     1081 atcgtctccg gcgcacccgc gagggtgtgc atggggcggg ttgcccgagt ggtcaaaggg
     1141 agcagactgt aaatctgccg gctcagccta cgaaggttcg aacccttcac ccgccacagg
     1201 cgccgcgacg gccctcgacc tctccggtcg ggggccgtcg ccgtacgccg cgagcgcccg
     1261 tcccccggcg tgccgtcagc ggcgcaggag gccgcggcga gcaggggctc ccggcagggc
     1321 cacgagcccg acggtcctgc cgacgacgcc caacggcctc ccggaccgcg tccacaggct
     1381 gtccacacag tgtgagcaag gcgctacgca tagttacttc ggatcacgag aggggcgaga
     1441 gcgggccatc ctcggcccgg gcgctccggg ccgtgcccga ggtcgtgtag tctctcctcg
     1501 gcccgcgccg cccccttagc tcagtcggca gagcgtctcc atggtaagga gaaggtctac
     1561 ggttcgattc cgtaaggggg ctctgtccac tctcggctcc ggccgtcggg tggctcgcct
     1621 cacggcggtg tagctcagcc tggt
//
LOCUS       DASWNJ010001177         1666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_657856, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001177 DASWNJ010000000
VERSION     DASWNJ010001177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657856"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VGB85_19845"
     CDS             <1..243
                     /locus_tag="VGB85_19845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136351.1"
                     /translation="GVEDDFTTAAAGLERLQSITGGTLATLQIDQLVTCGRTVGAFLS
                     DPNSEGALAVMLRADPHGDIESLTAVWSPTPVVLSP"
     gene            complement(244..1008)
                     /locus_tag="VGB85_19850"
     CDS             complement(244..1008)
                     /locus_tag="VGB85_19850"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0136352.1"
                     /translation="MDPSLWLPNADLRPTARLRLLCFAHAGGGAAPFFRWNQVLPAQI
                     AVCPVRRPGRESAHAEPLLRDIKAIAAGSVRALQSLPPLPTVLLGHSLGGLVAYEVAR
                     LMQSLGATPELLIVSAKPPPHVPSLLPNLSHLPNDRFLRELDELYGGIPKEIKAVPEL
                     LEMMLPVLRADMAASENYRHTPGPRLRCPIVACHGDADRAVDPTTQGAWGELTDCGLT
                     RRTFPGGHFYVYEPGSGFLAWLRDELSEELRARLPA"
     gene            1137..>1666
                     /locus_tag="VGB85_19855"
     CDS             1137..>1666
                     /locus_tag="VGB85_19855"
                     /inference="COORDINATES: protein motif:HMM:NF013161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY0136353.1"
                     /translation="MTSLELLADPDDARIICRCVPVTLGTLRQAMQRGCQDFDALRRE
                     TRCGSVCNSCVPTVLELLGEDVWVVAEVVAVHAHAADVRSIELAPRSADYPQPRPGQH
                     IILEAQLDDARTLRRTYTISSAARDSRLRLTIKREPQGVFTGWLFATCSPGATLRITR
                     PRGDFILDHAPGPVVC"
BASE COUNT          241 a          633 c          569 g          223 t
ORIGIN      
        1 ggcgtcgagg acgacttcac gaccgccgcc gccggcctcg agcgcctcca gtcgatcacc
       61 gggggcacgc tcgccaccct gcagatcgat cagctcgtca cctgcgggcg caccgtcggc
      121 gccttcctgt ccgacccgaa cagcgagggc gcgctcgccg tgatgctgcg cgccgacccg
      181 catggcgaca tcgagtcgct caccgccgtg tggtcgccga cgccggtcgt cctgtcgccc
      241 tgactacgcg ggcaggcgcg cgcgcagctc ctcgctcaac tcgtcacgta gccaggcgag
      301 gaagcccgag cccggctcgt acacgtagaa gtgtccgccg gggaaggtcc tgcgcgtgag
      361 cccgcagtcg gtcagctcgc cccacgcgcc ttgcgtcgtc gggtcgacgg cccggtcggc
      421 gtcgccgtgg cacgcgacga tcgggcagcg cagccgcggc cccggcgtgt ggcgatagtt
      481 ctcggacgcg gccatgtccg cgcgcagcac cggcaacatc atctcgagca gctcgggcac
      541 cgccttgatc tccttcggga tcccgccata cagctcgtcg agctcgcgca ggaaccggtc
      601 gttcggcaag tgcgagaggt tcggcaagag cgacggcacg tgcggcggcg gcttggcgga
      661 cacgatcagc agctccggcg tcgcgccgag cgactgcatc agccgcgcga cctcgtaggc
      721 gaccagaccg ccgaggctgt gcccgagcag caccgtcggc agcggcggca agctctgcag
      781 cgcccgcacc gagccggccg cgatcgcctt gatgtcgcgc agcagcggct ccgcgtgcgc
      841 gctctcccgc ccgggccgtc gcaccggaca caccgcgatc tgtgccggca gtacttggtt
      901 ccagcgaaaa aacggcgccg cgccgccgcc cgcgtgcgcg aagcacaaca gccgcagccg
      961 cgcggtcggc cgcagatcgg cgttcggtag ccagagagaa gggtccacgc ggggcgcaga
     1021 gaaacagcga acgccgcgag tgtcaactcg cgacgccatc gcgcgcccgc gcaaccgctg
     1081 cacgcgcgag agcgcagctt gtgagcgccg cgtcgagcgg cctaccatcg cgccgcatga
     1141 catcgctgga gttgctcgca gaccctgatg acgctcgcat catttgtcgc tgcgtccccg
     1201 tcacgctcgg gacgctgcgc caggcgatgc agcgcggctg ccaggacttc gacgcgctgc
     1261 gccgcgagac ccgctgcggc tcggtgtgca acagctgcgt gccgaccgtg ctcgagctgc
     1321 tcggtgagga cgtgtgggtc gtcgccgagg tcgtcgccgt gcacgcgcac gccgccgacg
     1381 tgcggtcgat cgagctggcc ccgcgcagcg ccgactaccc gcagccgcgg cccggtcaac
     1441 acatcatcct cgaggcgcag ctcgacgacg cgcggacgct tcgtcgcacg tacacgatct
     1501 cgtccgcggc ccgcgactcg cggctgcgcc tgacgatcaa gcgcgagccg cagggcgtgt
     1561 tcaccggctg gctgttcgcg acgtgcagcc ccggcgcgac cctgcggatc acccggccgc
     1621 gcggcgactt catcctcgac cacgcgcccg gccccgtcgt gtgcat
//
LOCUS       DASWNJ010001178         4781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_552620, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001178 DASWNJ010000000
VERSION     DASWNJ010001178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552620"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VGB85_19860"
     CDS             <1..238
                     /locus_tag="VGB85_19860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136354.1"
                     /translation="PGPAHAEIHAHNPEMTLETMTSAAAALAPLAAVAGGRPGAMTPA
                     RWQLLADQLAELGVIAPGSVDPTGAFTTAFLPGV"
     gene            287..964
                     /locus_tag="VGB85_19865"
     CDS             287..964
                     /locus_tag="VGB85_19865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0136355.1"
                     /translation="MIDLYTAATPNGHKISIALEEMALPYTVHALSLTAGDQKQPAFL
                     EINPNGRIPAIVDRDNDDFAVFESGAILVYLAEKTGQFLPTDVKGRSRVMQWLMFQMG
                     GVGPMMGQANVFFRYAPEKIPYAIGRYQREVRRLFEVLDRQLGREAHLAGDYSIADMA
                     TWPWVRAYEWSGVEIDGLDNLQRWLDAVGERPAVVRGKEVPTASPLAAGQSRDELAKN
                     VRDNLLA"
     gene            complement(972..2948)
                     /locus_tag="VGB85_19870"
     CDS             complement(972..2948)
                     /locus_tag="VGB85_19870"
                     /inference="COORDINATES: protein motif:HMM:NF036933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HEY0136356.1"
                     /translation="MRTRHATLAFVLGVAACGDSGTTSATTEGTTGDTTAATDPSSPI
                     STPTSTPMTGEPTGTTVVTDGSVSETIGTTVTTTTDTTAGPTTESDASSDTTMGVVTQ
                     TTGDTTNTGTTGDTTGGGCPQGQVVCEGDIAKTCDGMGGFTDETPCALGCVEGEGCVM
                     CEPGAKSCEGEISKICNETGTAEDEFLCDGVQGVTCDPNAGECVGACSPNALGKSYIG
                     CDYYPTITANIVTDNFSFAVVVANTSNQTANLEIDKAGKLVLNDSVGPQSVKVIKLPW
                     DYALKGDDVMPVSMLVPGGAYRLRSDQPVTVYQYNPIEYEIDFFDNSVTNDASLLLPV
                     NVWTGDYFVAARNTWDAVGFGQIVPGFYTVTASEDDTTVTLLPSETGNKVLAGGGVKA
                     DGTGQVKLDRGDALQIFSALINGNPSAADVTGTRITADKKIQVIGGHICTFIPYDIGY
                     CDHLEESMFPYETLAKKYIITPALIPTGGDVPKAQMVRIIATEDNTTLTYDPPQNGGP
                     AMIAKAGQYVEIADTVADFEVTSNNKILISQYMQGQDAGGDSGDPAMALAVATEQYRK
                     SYLFHAPINYEVNYVNITAPLDAKVTLDNVAVAGLKPIGATGLGVARVKLGNNVDGNH
                     DVKADKPIGISVYGYGQYTSYWYPGGLDLEPLPQ"
     gene            complement(3024..4229)
                     /locus_tag="VGB85_19875"
     CDS             complement(3024..4229)
                     /locus_tag="VGB85_19875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136357.1"
                     /translation="MAGRTNRSREAATSEVGADTDSLYGLPLAEFTAARDRMAKALAG
                     AGDKREAARIKALRKPATAAWAANQLARRHPDELAGLLKAGDELRAAQRSLRPSGAGA
                     LREASERLRRAVAELSGTAEEVLAEAGFSASGKHVEGVRRILQATAAASESERARLRR
                     GVLTEELQAADFTDVLGMMSGQVFAGGDEDAEEEEEDAPEPARGRKKAGDEARAADAG
                     RGKAGAKQAPAKAAAKAATKATTKAAAKATKKTAAKATTKPAAKATTKTATKQADAKA
                     AAREKQAAAKAKQAAAKQAAREAAARAKEEAREGREAAQAERATRASARRARDVASRE
                     AQAQARQAERAAAKAQREREVAEQAEARAQEARARAEEAGRAAEEAQARADEAAQRLD
                     EAQAALDGA"
     gene            complement(4282..4578)
                     /locus_tag="VGB85_19880"
     CDS             complement(4282..4578)
                     /locus_tag="VGB85_19880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136358.1"
                     /translation="MRLTDEAALPVVAGVLQVRREVGLVTYPRGKSAMILYAGVEDVR
                     AACRRLAAEHPGRAWLCRWNRDPVEDVAGAAARLLADFAGRFGAEPSRMYEGED"
     gene            complement(4593..>4781)
                     /locus_tag="VGB85_19885"
     CDS             complement(4593..>4781)
                     /locus_tag="VGB85_19885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136359.1"
                     /translation="ERRPEIEAAQAGQMRAYGMVQKPMLVQHVMFSMLGLVMRMKKFA
                     MPAPPVVEARYAVACEAA"
BASE COUNT          582 a         1839 c         1558 g          802 t
ORIGIN      
        1 ccccggcccc gcgcacgcgg agatccacgc gcacaacccc gagatgaccc tcgagacgat
       61 gaccagcgcc gccgcggccc tcgccccgct cgccgcggtc gcgggcggtc gacctggcgc
      121 gatgaccccc gcgcgctggc agctgctcgc cgaccagctc gccgagctcg gcgtgatcgc
      181 gccgggctcc gtcgacccga cgggcgcctt cacgaccgcg ttcttgccgg gcgtttgacc
      241 gcgcgtcagc ccgggccggg gccggggtta cactgccagc gccgccatga tcgacctcta
      301 taccgccgcg acacccaatg gccacaagat ctccatcgcg ctcgaggaga tggccctccc
      361 ctacaccgtg cacgcgctgt cgctcaccgc gggcgatcag aagcagcccg cgttcctcga
      421 gatcaacccc aacgggcgga tcccggcgat tgtcgatcgc gacaacgacg acttcgcggt
      481 gttcgagtcc ggcgcgatcc tcgtgtacct cgccgagaag accgggcagt tcctcccgac
      541 cgacgtcaag ggccgctcgc gcgtgatgca atggctgatg ttccagatgg gcggcgtcgg
      601 cccgatgatg ggccaggcca acgtgttctt ccgctacgcc cccgagaaga tcccgtacgc
      661 gatcggccgc taccagcgcg aggtccggcg cctgttcgag gtgctcgacc gccagctcgg
      721 ccgcgaggcc cacctcgctg gcgactactc gatcgccgac atggcgacct ggccgtgggt
      781 gcgcgcgtac gagtggtccg gcgtcgagat cgacggcctc gacaacctcc agcgctggct
      841 cgacgcggtc ggcgagcgcc cggccgtggt ccgcggtaag gaggtcccga ccgcctcgcc
      901 gctcgccgcc ggccagagcc gggacgagct ggcgaagaat gtgcgcgaca acctgctcgc
      961 gtgacggttc gttactgcgg cagcggttcc aggtcgaggc cgcccgggta ccagtagctc
     1021 gtgtactggc catagccgta cacgctgatc ccgatcggct tgtcggcctt gacgtcgtgg
     1081 ttgccgtcga cgttgttgcc gagcttgacg cgggcgacgc cgaggccggt cgcgccgatc
     1141 ggcttgaggc ccgcgacggc gacgttgtcg agcgtcacct tggcgtcgag cggcgcggtg
     1201 atgttgacat agttgacctc gtagttgatc ggcgcgtgga acaggtagct cttgcgatac
     1261 tgctcggtgg cgaccgccag cgccatcgcg gggtcgcccg agtcgccgcc ggcgtcctgg
     1321 ccctgcatgt actggctgat caggatcttg ttgttcgagg tcacctcgaa gtcggccacg
     1381 gtgtcggcga tctcgacgta ctggccggcc ttggcgatca tcgccggccc gccgttttgc
     1441 ggcggatcgt aggtcagcgt cgtgttgtcc tcggtggcga tgatgcgcac catctgcgcc
     1501 ttgggcacgt cgccgccggt cgggatcagc gccggcgtga tgatgtactt cttcgccagc
     1561 gtctcgtacg ggaacatcga ctcctcgagg tgatcgcagt agccgatgtc gtacggaatg
     1621 aaggtacaaa tgtggccgcc gatcacctgg atcttcttgt cggcggtgat ccgcgtgccg
     1681 gtgacgtcgg cggcgctcgg gttgccgttg atcagcgcgg agaagatctg cagcgcgtcg
     1741 ccgcggtcga gcttgacctg gccggtgccg tcggccttca ctccgccgcc cgcgagcacc
     1801 ttgttgccgg tctcggaggg cagcagcgtg accgtcgtgt cgtcctcgct ggccgtcacg
     1861 gtgtagaagc cgggcacgat ctggccgaag ccgaccgcgt cccaggtgtt gcgcgcggcg
     1921 acgaagtagt cgcccgtcca cacgttgacc ggcagcagca gcgaggcgtc gttggtgacc
     1981 gagttgtcga agaagtcgat ctcgtactcg atcgggttgt actgatacac cgtgaccggc
     2041 tggtcggacc gcaggcggta ggcgccgccg gggaccagca tcgacaccgg catgacgtcg
     2101 tcgcccttga gcgcgtagtc ccacggcagc ttgatcacct tgacgctctg cggcccgacg
     2161 ctgtcgttga ggaccagctt gcccgccttg tcgatctcga ggttggccgt ctggttggag
     2221 gtgttggcga cgacgaccgc gaagctgaag ttatcggtga cgatgttggc ggtgatcgtc
     2281 gggtagtagt cacagccgat gtagctcttg ccgagcgcgt tgggcgagca cgcgccgacg
     2341 cactcgcccg cgttcgggtc gcaggtgacc ccctgcaccc cgtcgcacag gaactcgtcc
     2401 tcggcggtgc cggtctcgtt gcagatcttc gagatctcgc cctcgcagct cttggccccc
     2461 ggctcgcaca tgacgcagcc ctcgccctcg acgcagccga gcgcgcacgg cgtctcgtcg
     2521 gtgaagccgc ccatgccgtc gcaggtcttg gcgatgtcgc cctcgcacac gacctgtccc
     2581 tgcggacagc cgccgccggt cgtgtcgccc gtcgtcccgg tgttcgtggt gtcgcctgtc
     2641 gtctgggtga cgacgcccat cgtcgtgtcg gacgacgcgt ccgactccgt cgtcggcccc
     2701 gccgtggtgt cggtggtcgt ggtgaccgtc gtgccgatcg tctcgctgac cgagccgtcc
     2761 gtgaccaccg tcgtgcccgt cggttcaccg gtcatcgggg tcgaggtcgg ggtcgagatc
     2821 ggcgacgagg ggtcggtggc cgcggtcgtg tcgccggtcg tgccctcggt ggtggcggac
     2881 gtggtgccgc tgtcaccgca agcggccacg ccgaggacga aagccagcgt cgcgtgacgc
     2941 gtgcgcatgc aagtaggggt cggagtcata cgcgcgaggc tggcagcttc gcccgcgctc
     3001 cgcaattcga gattgcggcc ccgtcacgcc ccgtcgaggg ccgcctgggc ctcgtccagc
     3061 cgctgcgccg cctcgtcggc ccgcgcctgc gcctcctcgg ccgcgcgccc ggcctcctcg
     3121 gcccgcgcgc gggcctcctg ggcccgggcc tccgcctgct cggccacctc gcgctcgcgc
     3181 tgcgccttcg cggccgcacg ctcggcctgc cgcgcctgcg cctgcgcctc gcggctcgcc
     3241 acgtcgcgcg cacgccgggc cgaggcccgc gtcgcccgct cggcctgcgc cgcctcccga
     3301 ccctcgcgcg cctcctcctt cgcccgcgcc gcggcctcgc gcgccgcctg cttcgccgcc
     3361 gcctgcttcg ccttcgcagc agcctgcttc tcgcgggcgg ccgccttcgc gtcggcctgc
     3421 ttcgtcgcag tcttcgtcgt cgccttcgcc gcgggcttcg tcgtcgcctt cgccgcggtc
     3481 ttcttcgtcg ccttcgccgc agccttcgtc gtcgccttcg tcgcagcctt cgcggccgcc
     3541 ttcgcaggtg cctgcttcgc tcccgccttc ccccgcccgg catccgcagc tcgcgcctcg
     3601 tcccccgcct tcttgcgccc ccgcgccggc tccggcgcgt cctcttcttc ctcctccgcg
     3661 tcctcatccc cgcccgcgaa cacctgcccg ctcatcatcc cgagcacatc ggtgaagtcg
     3721 gcggcttgca gctcctccgt cagcacgcca cggcgcaggc gcgcccgctc gctctcggag
     3781 gccgcggcgg tcgcttgcag gatcctccgc accccctcga cgtgcttccc ggacgccgag
     3841 aaccccgcct ccgcgagcac ctcctcggcg gtcccggaca gctcggccac cgctcgtcgc
     3901 agccgctcgc tcgcctcccg cagcgcccct gcgccggacg gccgcaggga tcgctgcgcc
     3961 gcgcgcagct cgtccccggc cttgagcagc cccgcgagct cgtccggatg ccgccgcgcg
     4021 agctgattgg ccgcccacgc cgcggtggca ggcttgcgca gcgccttgat ccgcgccgcc
     4081 tcgcgcttgt cgcccgcgcc cgccagcgcc ttcgccatcc gatcacgcgc cgcggtgaac
     4141 tcggcgagcg gcagcccgta cagcgagtcg gtatcggcgc cgacctccga ggtcgcggcc
     4201 tcgcggctcc tgttcgtgcg gccggccatg cgttcacgtt cctattacac gtgaagcgcg
     4261 ctcacggcca cgcccccgcg ctcaatcctc gccctcatac atccgcgagg gctccgcccc
     4321 gaagcgcccg gcaaagtccg cgagcaaccg cgccgccgcc ccggcgacat cctccaccgg
     4381 atcccgattc caccgacaca accacgcccg ccccggatgc tccgccgcga gccgccgaca
     4441 ggccgcgcgc acgtcctcga cgcccgcgta caggatcatc gcgctcttgc cccgcggata
     4501 cgtgacgagc ccgacctcac gccgcacctg cagcaccccc gccaccaccg gcagggccgc
     4561 ctcatcggtc aggcgcaccc agggtccgaa cctcacgccg cctcgcaggc gaccgcgtac
     4621 cgggcctcga cgaccggcgg ggccggcatc gcgaatttct tcatacgcat gaccagaccg
     4681 agcatcgaga acatcacatg ctggaccagc atcggctttt ggaccatccc atacgcgcgc
     4741 atctggccgg cctgggccgc ctcgatctcc ggccggcgct c
//
LOCUS       DASWNJ010001179         1511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_618204, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001179 DASWNJ010000000
VERSION     DASWNJ010001179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618204"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1511)
                     /locus_tag="VGB85_19890"
     CDS             complement(<1..>1511)
                     /locus_tag="VGB85_19890"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0136360.1"
                     /translation="PAPATSARCSCELLLSLCDGAEVQACLSTFAVAVADLRRGIAEL
                     HAETEAEPADWRVRIGQRSAARKGAGELELLLAIVRSADCHAYQLLERAGVRCGALRK
                     RLIDQLREAENLAAHAVAPRAPHRGRQSQLANQRPPATREARVLPIRGDTRASDTRTT
                     SEPGRRGSGLEDSSTRHRLAADDLPTRQSRDDSATRHRLAADDLPTRQHRGASEDSSP
                     RHRLLDEGPRRHRLAEDSSTRLSAPVPEDSRRPPAPRPRLLAPPAEPERARPRVLVYH
                     DEPEPYREEPDTSAHARPDPQPAADPGPRPELRPGPRLEPIDPRDLPPLHGREATLAR
                     LADAVLRRSPRPPLLVGPHGCGRTLVASHLARILGVPVFLLSAASHADEDTLRADLAV
                     VARQRGVAILDDLDQVGSDAPPIFLPALVKIWSSGQPPILTIVSPEGRARLETWLPGL
                     SSTFDVLELPVLEGDELRAAVAAAAPAVLAQHGLSLGTGSRLAELTRVADRFL"
BASE COUNT          166 a          496 c          678 g          171 t
ORIGIN      
        1 gaggaagcgg tcggcgacgc gggtcagctc ggcgagcctc gagcctgtcc cgagggacag
       61 gccgtgctgg gcgagcaccg cgggcgcggc ggcggcgacg gcggcgcgca gctcgtcacc
      121 ctcgagcacg ggcagctcga gcacgtcgaa ggtcgaggac aggccgggca gccaggtctc
      181 gaggcgcgcg cggccctcgg gcgagacgat cgtcaggatc ggcggctgtc ccgaggacca
      241 gatcttgacg agcgcgggga ggaagatcgg cggggcgtcg ctgccgacct ggtcgaggtc
      301 gtcgaggatc gccacgccgc gctggcgcgc gacgacggcg aggtcggccc gcagggtgtc
      361 ctcgtcggcg tgactggcgg cgctgagcag gaacaccggg acgccgagga tccgcgcgag
      421 gtggctggcg acgagcgtgc gcccgcagcc gtgggggccg acgagcaagg gcggccgcgg
      481 cgagcggcgc agcacggcgt cggcgaggcg ggcgagcgtc gcctcgcggc cgtgcagcgg
      541 cggcaggtcg cgggggtcga tcggctcgag gcgggggccc ggacgcagct cggggcgcgg
      601 gccgggatcg gcggcgggct ggggatcggg gcgcgcgtgg gccgaggtgt cgggctcctc
      661 gcgatatggc tcgggctcgt cgtgatagac gagcacgcgc gggcgcgcgc gctcgggctc
      721 ggccggtggc gcgagcaggc gtgggcgcgg ggcgggcggg cggcggctgt cctccgggac
      781 aggtgcggag agccgggtcg agctgtcctc ggcgaggcga tgccggcgcg ggccctcgtc
      841 gagcagacgg tggcgcggcg agctgtcttc ggaggcgccg cggtgctgcc gggtcgggag
      901 gtcgtcggca gcgaggcggt gacgcgtcgc gctgtcgtcg cgggactgcc gggtcgggag
      961 gtcgtccgcg gcgaggcggt ggcgggtcga gctgtcctcg aggccagatc cgcggcggcc
     1021 cggctcgctc gtcgtgcgcg tatcactggc gcgggtgtcc ccgcggatcg gcagcacacg
     1081 cgcctcgcgg gtggcaggcg gccgctggtt cgcgagctgg ctctggcgcc cgcgatgcgg
     1141 cgcgcgcgga gcgacggcgt gggcggcgag gttctcggcc tcgcgcagct ggtcgatcag
     1201 ccgcttgcgc agggccccgc agcgcacgcc ggcgcgctcg agcagctggt aggcgtggca
     1261 gtctgcgctg cggacgatcg ccagcagcag ctcgagctcg cccgcgccct tgcgcgcggc
     1321 gctgcgctgg ccgatccgga cccgccagtc ggcgggctcg gcctcggtct cggcgtgcag
     1381 ctcggcgatc ccgcggcgca ggtcggcgac ggcgacggcg aaggtggaca ggcacgcctg
     1441 cacctcggcg ccgtcgcaca gcgagagcag cagctcgcag gagcagcggg cgctcgtggc
     1501 gggcgcgggg g
//
LOCUS       DASWNJ010001180         5920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_403917, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001180 DASWNJ010000000
VERSION     DASWNJ010001180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5920
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403917"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VGB85_19895"
     CDS             complement(<1..377)
                     /locus_tag="VGB85_19895"
                     /inference="COORDINATES: protein motif:HMM:NF017739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HEY0136361.1"
                     /translation="MKDLWDMATSAKGVLPQVEYSFRSEAWRGAPWHVRRVELTEALS
                     DPYRLRLDLVVDDLDGAVDPLLGAGCELTIGRGAAARSVCGLVLELDDLGVVGGRRLV
                     RMHIGPALALLAQRVNTRAWQDR"
     gene            718..1212
                     /gene="tssD"
                     /locus_tag="VGB85_19900"
     CDS             718..1212
                     /gene="tssD"
                     /locus_tag="VGB85_19900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HEY0136362.1"
                     /translation="MAEFVHLELYANDERIEGESTQTSLGREGTIECFNLDHKIRSSI
                     DPVSGRAAGRRVYEEFKINKRLDSSTPLIIRALCNNEEIRGIFRFFRPHHSGDGTTEH
                     FFTVEVSEARIASYRLLSPNASDRNNADPPMEEVAFVFHNIRWCDEANHKEHADSWKE
                     GGSR"
     gene            1376..1768
                     /gene="tssE"
                     /locus_tag="VGB85_19905"
     CDS             1376..1768
                     /gene="tssE"
                     /locus_tag="VGB85_19905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HEY0136363.1"
                     /translation="MSGGLLQRIDALGLGRVDDEVTAILTHVRVLLGTRQGSSASTPG
                     YGLQDITDAIHSYPVGAQQIGSRIRAAIEAFEPRLARGVTVELLDSDSPLQLDYRIVA
                     RLASDRRRLLVFHACVSPDREIALNLEG"
     gene            1772..3547
                     /gene="tssF"
                     /locus_tag="VGB85_19910"
     CDS             1772..3547
                     /gene="tssF"
                     /locus_tag="VGB85_19910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HEY0136364.1"
                     /translation="MFAKFFESELAYLRERGAEFASGPYQAAARLLDRGGDPDVERML
                     EGFAFLTARIRERLEDAVPELVENLVELFLPHMLRPLPATAIVQFSTNIHALRGPQKI
                     ARGRPLEAPTRHGVPCRFRTAYDLELLPIDVASAEVDRTLPSAPALVLRLRLTEASRA
                     LLAQPRELRFFIAGEPALTYALRLLLLSGACKSLRSRLLPAAGPPANAPPGQELGPEA
                     IVPVGFADDEALVPWPVLSHPGLRLVQEYMFAPEKFSFFGVRGPFFAEGKGELLELRF
                     QFDGHEVLPARVSADNFRLHCTPAINLFDITADPVRLDPLVHEHRLQAAGLRPDHAEV
                     YAIQSVRSSRAGRADERVYTPFFSYAHAQQPGALYYSARRSRSPVDGNIDLYLAVHAG
                     QPGHAGPDSATRPAPEQLGEILHVDMLCTNRMLAADLGAGAINREPRAGALQLRSITP
                     ITPPVPAPLGSELYWRLLSHLSLGQASLADATVLRNMLGLYDFQAGSPRSAAARIAAI
                     RKVETRAVTRLVSGAPVRGTETNLELDEAGFPGPGEAHLLLSVLDALFADSVGINSFN
                     QVTARLHPSDARFRWPARNGNLAVI"
     gene            3511..4542
                     /gene="tssG"
                     /locus_tag="VGB85_19915"
     CDS             3511..4542
                     /gene="tssG"
                     /locus_tag="VGB85_19915"
                     /inference="COORDINATES: protein motif:HMM:NF018675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HEY0136365.1"
                     /translation="MAGPQRQPGSDLSRVLPLLRSAGRYGFLAVVDLLERDGGLPIRF
                     RHDDSMSFHPGELRSVTEIELPPATADGPPRRAFEVTACFLGLTGSFGPVPVYLSEMT
                     VCDDESSGLRRALLAPFHHRLYELYHRAARRCDPPRSFAAGATDLWSRRLLAWLGVGT
                     VDLRHIPPVALLRLAPLLVSRVRSARTLHLALREVLSDLLPAGAELRIVQFTGGHAPL
                     SAGTQMQLGQRDTLALGRSTVIGTSCRDPAGGLRIEVGPLAAAQVAAFSPRGVAFARI
                     AELVKLLVRGPLDVELDLLTDSAASSLPLGQLRLGSTFRLVAPDGARRRHLRFNLSEE
                     TATRIAARA"
     gene            4617..>5920
                     /locus_tag="VGB85_19920"
     CDS             4617..>5920
                     /locus_tag="VGB85_19920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0136366.1"
                     /translation="MRVDPKALVRRLDPACTQALEEAVASAASGRFYEIVVEHVLLAL
                     LEPADGEAAAVLTEVRRDRRRATTTLQNVLQRMRTGNAGRPVFSEGVFQLFEEAWLVA
                     SVEHGRQLLRPADVLLEFATRGNRYTAETIPELTGIPLDELRRIVASYSEQQPAAPTA
                     TQGAAPGAAGAPGAAPGAAAAGGGREMLARFTVSYTDRAKAGKIDPIFGRDAEIRQMI
                     DILARRRKNNPILVGEPGVGKTALVEGLARAIVAGDVPSGLRDVELLGLDLGLLMAGA
                     GVRGELENRLKKVIAEVKSSATPNILFIDEAHTIMGASGDSAELPNLLKPELARGELR
                     TLAATTWSEYKKYFEKDAALARRFQLVKVGEPDQETAILMLRGLRDMYSKAHGVVIRD
                     EAVVAAVELSERYISGRQLPDKAIDLIDTASARVKIEQDAPP"
BASE COUNT          855 a         2410 c         1769 g          886 t
ORIGIN      
        1 ctgcggtcct gccacgcgcg cgtgttgacc cgctgggcca gcagcgccag cgccggcccg
       61 atgtgcatgc ggaccaggcg acgtccgccg acgaccccga ggtcatcgag ctcgagcacc
      121 agcccacaca ccgaccgcgc cgccgcgccg cgaccgatcg tcagctcgca tcctgccccg
      181 agcaaggggt ccaccgcgcc gtcgaggtcg tcgaccacga ggtcgaggcg caggcgatac
      241 gggtccgaca gcgcctccgt cagctcgacg cgccgcacgt gccacggcgc cccacgccag
      301 gcctcgctac gaaagctgta ctcgacctgc ggcagcacgc ccttggcgct cgtcgccatg
      361 tcccagaggt ctttcacacg cccgcccatc tcatcaccat atcgtagtct gctcgccgct
      421 caccataggc cctcagtcgc cggccacgct cgcctcttcg gcgcacatgc gcgcatcgct
      481 tacggcgccc gtcacgctgc gccaggggcc gatcacggcc ccaccacgtc gactccgctg
      541 cgatctgccg gggccgcgaa accccgacgc tgcagcatca cgcacacagc ctttcagggc
      601 cctcgtgtgc tcatcgatca ctaggatcgc atcacacgcg tgatcatgtc acaccgcgct
      661 tacccgctcc gtcgatcgcc tcttgcgaca ccttgtgatt ttgtctagcc ttcaaaaatg
      721 gccgaattcg ttcatctaga gctgtacgcc aacgacgagc gcatcgaggg tgagtcgaca
      781 cagaccagcc tcgggcgcga gggcaccatc gagtgcttca acctcgatca caagatccgg
      841 agctccatcg atccggtctc gggacgcgcc gccggtcgtc gcgtctacga ggagttcaag
      901 atcaacaagc gcctcgactc ctcgaccccg ctcatcatcc gcgccctgtg caacaacgag
      961 gagatccggg gcatcttccg cttcttccgt ccgcatcact ccggcgacgg caccaccgag
     1021 cacttcttca ccgtcgaggt cagcgaggcc cgcatcgcct cctaccgcct gctctccccc
     1081 aacgccagcg accgcaacaa cgccgacccg ccgatggaag aggtcgcctt cgtgttccac
     1141 aacatccgct ggtgcgacga ggccaaccac aaggagcacg ccgactcctg gaaagagggc
     1201 ggctcccgct gagcccccgc cgcgggccgc tcgcccgcag ccgttgaacg cccggcctgg
     1261 ccggctcgga cgcgccctgc cgcgtccgcg ctcgctggcc gttcttgctc tcggctctct
     1321 cggcgctccg gtcgccgccg cacacgaggc acgaaatgca gtataaaaac agaagatgtc
     1381 cggcggcctc ctccaacgca tcgacgcgct cggcctcggc cgcgtcgacg acgaggtgac
     1441 cgcgatcctc acccacgtgc gcgtgctgct cggcacgcgc cagggctcgt ccgcatcgac
     1501 gcccggatac ggcttgcagg acatcaccga tgcgatccac agctatcccg tcggcgccca
     1561 gcagatcggc agccggatcc gtgccgcgat cgaggcgttc gagccgaggc tcgcgcgtgg
     1621 tgtcaccgtc gagctgctcg acagcgactc gccgctgcag ctggactatc gcatcgtcgc
     1681 caggctcgcc tccgaccgcc ggcgcctgct ggtgttccac gcctgcgtct cgcccgaccg
     1741 cgagatcgcg ctgaacctcg agggctaagc cgtgttcgcc aaattcttcg agagtgagct
     1801 cgcctatctc cgtgaacgcg gggccgagtt cgcctcgggg ccgtaccagg cggccgcgcg
     1861 gctgctcgac cgcggcggcg acccggacgt cgagcgcatg ctcgagggct tcgccttcct
     1921 gaccgcgagg atccgcgagc gcctcgagga cgccgtgccc gagctcgtcg agaacctcgt
     1981 cgagctgttc ctgccgcaca tgctgcgacc gctgcccgcg accgcgatcg tgcagttctc
     2041 gaccaatatc catgcgctgc gcggcccgca gaagatcgcc cgcggccggc cgctcgaggc
     2101 gccgacccgc cacggcgtac cctgtcgctt ccgcaccgcc tacgacctcg agctgctgcc
     2161 gatcgatgtc gccagcgcgg aggtcgaccg caccctcccg agcgcgcctg ccctcgtgtt
     2221 gcgcctacgg ctgaccgagg ccagccgcgc cctgctcgcc cagcctcgcg agctgcgctt
     2281 cttcatcgcc ggcgagcccg ccctgaccta cgcgctgcgc ctgctcctgc tctccggcgc
     2341 ctgcaagtcg ctgcgcagcc gcttgctgcc cgccgccggt ccgcccgcca acgccccacc
     2401 cggccaggag ctcggccccg aggcgatcgt cccggtcggc ttcgccgacg acgaggcgct
     2461 cgtcccctgg cccgtgctct cgcatccggg cctgcggctc gtccaggagt acatgttcgc
     2521 gccggagaag ttctccttct tcggcgtgcg cggcccgttc ttcgccgagg gcaagggcga
     2581 gctgctcgag ctgcgcttcc agttcgacgg tcacgaggtc ctgccggccc gcgtctccgc
     2641 cgacaacttt cgcctgcact gcacgcccgc gatcaacctc ttcgacatca ccgccgaccc
     2701 cgtccgcctc gatccgctcg tgcacgagca tcgtctacag gctgcgggcc tgcgtcccga
     2761 tcacgcggaa gtctacgcga tccagtcggt gcgcagcagc cgggccggtc gcgccgacga
     2821 gcgcgtctat acccccttct tcagctacgc ccacgcccag cagcccggcg ccctgtacta
     2881 cagcgcccgc cgcagccgct cgccggtcga cggcaacatc gacctctacc tcgccgtcca
     2941 cgccggacaa cctggtcatg caggccccga cagcgcgacg cgccccgccc cggagcagct
     3001 cggcgagatc ttgcacgtcg acatgctgtg caccaaccgc atgctcgccg ccgacctcgg
     3061 cgccggcgcg atcaatcgcg agccccgcgc tggcgcgctg cagctgcggt cgatcacgcc
     3121 gatcaccccg cccgtgcccg caccgctcgg cagcgagctg tactggcgcc tgctctcaca
     3181 cctgtctctc gggcaagcct cgctcgccga cgccaccgtg ctgcgcaaca tgctcggcct
     3241 ctacgacttc caggccggca gcccgaggtc cgccgccgcc cgcatcgcag cgatccgcaa
     3301 ggtcgagacc cgcgccgtca cccgcctcgt cagcggcgcg cctgtccgcg gtaccgagac
     3361 caacctcgag ctcgacgagg ccggcttccc cggccccggc gaggcccacc tgctgctcag
     3421 cgtcctcgac gcgttgttcg ccgacagcgt cggcatcaac agcttcaatc aggtgaccgc
     3481 caggctccac ccctctgacg cgaggttccg atggccggcc cgcaacggca acctggcagt
     3541 gatctgagcc gcgtgctgcc gctgctgcgc agcgccggtc gctacggctt cctcgccgtc
     3601 gtcgacctgc tcgagcgcga cggcggcctc ccgatccgct ttcgccacga cgacagcatg
     3661 tcgtttcacc ccggcgagct gcgcagcgtc accgagatcg agctgccacc cgccaccgcg
     3721 gacgggccgc cgcgccgcgc cttcgaggtc accgcctgct tcctcggcct gaccggctcg
     3781 ttcggacctg tccctgtgta cctgtccgaa atgacagtgt gcgacgacga gagctccggc
     3841 ctgcgccgcg ccctgctcgc cccgttccac caccgcctct acgagctcta ccaccgcgcc
     3901 gcgcggcgct gcgaccctcc gcgcagcttc gccgccggcg cgaccgacct gtggtcgcgc
     3961 cgcctgctcg cctggctcgg cgtcggcacc gtcgacctgc gccacatccc cccggtcgcg
     4021 ctgctgcgcc tcgccccgct gctcgtctcg cgcgtgcgat cggcccgcac cctgcacctc
     4081 gccctgcgcg aggtcctgtc cgacctgctg cccgccggcg ccgagctgcg gatcgtccag
     4141 ttcaccggcg gccacgcgcc gctcagcgcg ggcacgcaga tgcagctcgg ccagcgcgac
     4201 accctcgcgc tcggccgctc gaccgtgatc ggcacctcgt gtcgtgatcc cgccggcggc
     4261 ctgcgcatcg aggtcggccc gctcgctgcc gctcaggtcg cggcattcag cccgcgcgga
     4321 gtcgcattcg cgcggatcgc cgagctcgtc aaattgctcg tccgtggccc cctcgacgtc
     4381 gagctcgacc tgctgaccga cagcgccgcc agcagccttc cactcggtca attacggctc
     4441 ggaagcacgt ttcgcctggt cgcgcccgat ggcgcgcgcc gccgtcacct ccgcttcaac
     4501 ctgtctgaag agacagccac gcgcattgct gcacgggctt gatctcgcgc cgatccaatc
     4561 gcattcacgg gtccggcggc ctgttacact cgcagccctt tccggagcaa agaccaatgc
     4621 gcgtcgatcc caaagccctc gtccgtcgcc tcgaccccgc gtgcacccag gccctcgagg
     4681 aggcggtcgc cagcgccgcc tccggccgct tctacgagat cgtcgtcgag cacgtgctgc
     4741 tcgccctgct cgagcccgcc gatggtgaag ccgccgccgt gctcaccgag gtccgccgcg
     4801 atcgtcgacg cgccaccacc accctgcaaa acgtcctcca gcgcatgcgc accggcaacg
     4861 ccggccggcc cgtgttctcc gagggcgtgt tccagctgtt cgaggaggcc tggctcgtcg
     4921 cctccgtcga gcacggccgc cagctcctgc gccccgccga cgtcctgctc gagttcgcga
     4981 cccgcggcaa ccgctacacc gccgagacga tccccgagct gaccggcatc ccgctcgatg
     5041 agctgcgccg catcgtcgcc agctacagcg agcagcaacc ggccgccccc acggcgaccc
     5101 agggcgccgc accgggcgct gcgggtgcgc cgggcgctgc accgggcgcc gccgcggcag
     5161 gcggcggccg cgagatgctc gcccgcttca cggtgtccta taccgaccgc gccaaggccg
     5221 gaaagatcga ccccatcttc ggccgcgacg ccgagatccg gcagatgatc gacatcctcg
     5281 cccgccgtcg caagaacaac ccgatcctcg tcggtgagcc cggcgtcggc aagacggcgc
     5341 tcgtcgaggg cctcgcccgt gcgatcgtcg ccggcgacgt gccctcgggc ctgcgggacg
     5401 tcgagctgct cggcctcgac ctcggcctgc tgatggccgg cgccggcgtc cgcggcgagc
     5461 tcgagaaccg cctgaaaaaa gtcatcgccg aggtcaagtc ctccgccacc ccgaacatcc
     5521 tcttcatcga cgaggcccac acgatcatgg gcgcctcggg tgacagcgcc gagctcccca
     5581 acctgctcaa gcccgagctc gcccgcggcg agctgcgcac gctcgccgcg accacctggt
     5641 ccgagtacaa gaaatacttc gagaaggacg ccgcgctcgc gcgtcgcttc cagctcgtca
     5701 aggtcggcga gcccgaccag gagaccgcga tcctgatgct ccgcggcctg cgtgacatgt
     5761 acagcaaggc ccacggcgtc gtcatccgcg acgaggccgt cgtcgccgcc gtcgagctgt
     5821 ccgagcgcta catctccggc cgccagctcc ccgacaaggc gatcgacctg atcgacaccg
     5881 cctccgcccg cgtgaagatc gagcaggacg cgccccccga
//
LOCUS       DASWNJ010001181         2344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_480150, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001181 DASWNJ010000000
VERSION     DASWNJ010001181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480150"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VGB85_19925"
     CDS             <1..892
                     /locus_tag="VGB85_19925"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY0136367.1"
                     /translation="SGSAGGSDVLTPAQVEAYERDGALVLPGFLAPETCAALITRAEQ
                     IAQARAEEGPRSVFSTAEQARRSDEWFLGSGDQVRCFFEEHAFTAEGTLKVAAARAIN
                     KIGHALHDLDPLFAAASYDPRVAEIAADLGLREPLAMQSMAIFKQPGIGGEVGCHQDG
                     TFLYTDPMTVLGFWIALEDATLDNGCLWIAPGEHRGPLRRRFRRAAGGGSEFEVLDPT
                     PLPGPEALHALPVAAGTLVLLHGLLPHFSGANRSPRRRLAYSLHCIETGAEYPASNWL
                     QRGPHMPPRRLADGPPALAR"
     gene            943..2118
                     /locus_tag="VGB85_19930"
     CDS             943..2118
                     /locus_tag="VGB85_19930"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEY0136368.1"
                     /translation="MLARSTVVSALVLSLCAAFACGGEAGKADPKAEAKAPAKVEPQP
                     PPVAEPAAPAEEYAWDLPRGLKAPEVPADNPMTAAKVELGHALFFDKRLSVDGSRSCY
                     SCHQNELGNADGRVKALGPLDKHLQRNTPTIWNVAYYAELYWDGRTKGLEKQMIGAWK
                     GGNMGVGEANLAAKATEIGALPEYAEKFRAVFGEGNVTPERVAQAVSAYERTLLCGAT
                     AWDSNSMPAEAQRGWELFRGKAACSTCHSGDNFSDGLYHRVGVGVPENGEGGDPGRFD
                     ATKADADKYKFRTPTMRNVSRTAPYFHDGSVADLRAAVKQMATGGNRKVKELDSNLAD
                     RGLTDAEIDDIVTFLKALDCPGKLEVIGDQTVAGITPGTSSPADAKGEAAVDKKKAG"
BASE COUNT          355 a          794 c          888 g          307 t
ORIGIN      
        1 ctcggggtcg gcgggcggtt ctgacgtgct gacaccggcg caggtggagg cgtacgagcg
       61 cgacggggcg ctggtgttgc cgggcttcct ggccccggag acatgcgccg cgctgatcac
      121 ccgcgccgag cagatcgccc aggcgcgggc cgaggagggg ccgcgatcgg tgttctcgac
      181 ggcggagcag gccaggcgca gcgacgagtg gttcctgggg tccggcgatc aggtgcgctg
      241 cttcttcgag gagcacgcat tcacggccga gggcacgctc aaggtcgcgg cggcacgggc
      301 gatcaacaag atcgggcacg cgctgcacga cctcgacccg ctgttcgcgg cggcctcgta
      361 cgacccgcgc gtcgccgaga tcgcggcgga cctcggcctg cgcgagccgc tggcgatgca
      421 gagcatggca attttcaagc agccggggat cggcggcgag gtcggctgtc atcaggacgg
      481 cacgttcctc tacaccgacc cgatgacggt gctcggcttt tggatcgcgc tcgaggacgc
      541 gaccctcgac aacggctgcc tgtggatcgc gccgggcgag catcgtgggc cgctgcgccg
      601 gcggtttcgc cgggcggccg gcggcggcag cgagttcgag gtcctcgacc cgacgccctt
      661 gccggggccg gaggcattgc acgccctgcc ggtcgcggcc ggtacgctgg tgctgctgca
      721 tggcctgttg ccgcacttta gcggggccaa ccgctcgccg cggcggcgcc tggcgtactc
      781 gctgcattgc atcgagaccg gcgcggagta tccggcgtcg aactggctgc aacgcgggcc
      841 gcacatgccg ccgcgtcggc tcgccgatgg gccgcccgcg ctggcgcgct gatgggccgc
      901 ccgcgctggc gcgctgatgg gcgctggtgt agcgtcgcgg ccatgctcgc tcgctccacc
      961 gttgtatctg cgctcgtcct ctcgctctgc gccgcgttcg cctgcggagg tgaggccggc
     1021 aaggccgacc cgaaggccga ggccaaggcg ccggcgaagg tcgagccgca gcctcctccg
     1081 gtggcggagc ccgcagcgcc ggccgaggag tacgcgtggg acctgccgcg cgggctcaag
     1141 gcgccggagg tgccggcgga caacccgatg acggcggcga aggtcgagct cgggcacgcg
     1201 ctgttcttcg acaagcggct cagcgtcgac ggctcgcgca gctgctacag ctgccaccag
     1261 aacgagctcg gcaacgcgga cggtcgggtc aaggcgctcg ggccgctcga caagcacctc
     1321 cagcgcaata cgccgacgat ctggaacgtc gcgtattacg ccgagctgta ctgggatggc
     1381 cgcacgaagg gcctcgagaa gcagatgatc ggcgcgtgga agggcggcaa catgggcgtc
     1441 ggcgaggcca acctcgcggc caaggcgacg gagatcggcg cgcttccgga gtacgcggag
     1501 aagttccgcg cggtgttcgg cgagggcaac gtgacgcccg agcgcgtcgc ccaggcggtg
     1561 tccgcctacg agcgcacgct gctgtgcggc gcgacggcgt gggacagcaa cagcatgccg
     1621 gcagaggccc agcgcggctg ggagctgttc cgcggcaagg cggcgtgctc gacctgtcac
     1681 agcggcgaca acttctcgga cgggctgtac caccgcgtcg gcgtcggcgt gccggagaac
     1741 ggcgagggcg gcgaccccgg ccgcttcgac gcgaccaagg ccgacgccga caagtacaag
     1801 tttcgcacgc cgacgatgcg taacgtcagc cgcacggcgc cgtacttcca cgacggcagc
     1861 gtcgcggacc tgcgtgccgc ggtgaagcag atggcgaccg gcggcaaccg caaggtcaag
     1921 gagctcgaca gcaacctcgc ggaccgcggc ctgaccgacg cggagatcga cgacatcgtc
     1981 acgttcctca aggcgctcga ctgccccggc aagctcgaag tgatcggcga ccagacggtc
     2041 gccggcatca cgccgggcac gagctcgccc gcggacgcga agggcgaggc cgcggtcgac
     2101 aagaagaagg ccggctgagc gggatcgatc gcgggatcga tcgcgggctc cgtgcgcgcc
     2161 tcgacgtgat cgccggccca cgcgatcaag gcggtgctgt gaccgcgtcg cgcggcgccc
     2221 ggtagaactc gacgaggaag cgcagcggct cgaccggctc gaccgcttcg accacttcga
     2281 tgtggtgcgg cagctcgggc gcgacgatgc cgggcgcgtc gggggtcagc agcgtgacga
     2341 ggcc
//
LOCUS       DASWNJ010001182         5853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_444029, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001182 DASWNJ010000000
VERSION     DASWNJ010001182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5853
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444029"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..1347)
                     /locus_tag="VGB85_19935"
     CDS             complement(232..1347)
                     /locus_tag="VGB85_19935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136369.1"
                     /translation="MSSPRLTNVSAVCLALQLTACQPEKAPGGGDDTAATTGTDGNSD
                     TGHADGTTGAATSTTTDAEDPTTGGPGPASDSDPGSTSTSTTGAPDPTGSDPVCGGTG
                     EDQPPGPLVGLGPADPDEVDAYGGEFGGRFSVTASRWAEDAQWQVSAFAEFFDVPEAS
                     ESVMCAVEVDPLTGIDDCGVRWYGGLGWGGAEVLPRSAGSATLLGPGWTEPMVMGMTP
                     QKYYDADILGSGHPAGWGEAYGITWAGERLPAGELPQFVTLPAELKITAPAGVSWTFG
                     DGPFELAWTGTGAAPLQLRAQFVEPEGHTTWFELVCKIADDGSFVVPAEVVTLLPPGW
                     SGLGGLRRAVSTTAEAADRTIVGVAAVAIDLEVAVAP"
     gene            1673..2701
                     /locus_tag="VGB85_19940"
     CDS             1673..2701
                     /locus_tag="VGB85_19940"
                     /inference="COORDINATES: protein motif:HMM:NF021456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HEY0136370.1"
                     /translation="MSELENLTGFAATCLPSMLPDDRQLTLVVVSGRFVLPRPDGREA
                     PRPCEEQGEVRLADEYAGEPDALLLLHEGQSAPTRSGTDLTMLGHAWAPGGKLATRSD
                     VELQVGRCHKRALVFGDRVWCRTMAGVAPSRPAAFESIPLSYRRCFGGTPEQPSTSVA
                     EAAEYNPVGLGLHESEREAIDRPVPNFEDPQSPLQRPTGRPRPAGFGPIARHWRPRRT
                     YAGTYDQAWVEHRAPLWPRDLDERFFSAAPPGLFAVPHLQGGEPVRIAGMSPHGDHAF
                     SLPRVRLRARFELADRSIRRDLVLDAVHFELDGSIFTMYWRTAIATDPLRVRAVVVRT
                     LDLWEQER"
     gene            2707..3723
                     /locus_tag="VGB85_19945"
     CDS             2707..3723
                     /locus_tag="VGB85_19945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136371.1"
                     /translation="MVVSIVGVGLACPVGLTSKTATAAIRAGISRFQEIDEVVGPGGP
                     VRASMLAQLGADRSRTERATFFARHALCEAVGGLGTIDALPCFFALPEPDLGPRINLE
                     DLLRGLADVPTRAGSPLRLSLRPEAVVTAGRAGVFAALEKAIATFVDGGATTALIGGF
                     DTLVDPETLDALAEQDRLLGRTNLDGIIPGEAAAFIVLSALDAPSRRSALARISVSAT
                     AHEPLSFARANDRASASDGLTSVFRSLRAAVDGRADRVYAGVTGEGYFGQEFAHASLR
                     NAALMPEPLRCEPVSDALGDIGAAAGAVCLVQAVRAAAGQRSLVYASSDGGLVGGCVV
                     EPTR"
     gene            complement(3736..4479)
                     /locus_tag="VGB85_19950"
     CDS             complement(3736..4479)
                     /locus_tag="VGB85_19950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136372.1"
                     /translation="MIVPFLLKFFAARAPMYSASLRGVSLDDIRLCEKNCSLSFPRTY
                     TDFLVAMGDECGDFHPFGPSQSCDFYELLESFSPDGYPDRYFKVAFESSPMAVTRFDF
                     FLDLQRSDGIDAPLVSFEEMDEFVPQAVQEVGFTLGEQIARRAFDVFEVDRRAFTRTL
                     RLLPASSAEARSAMKLAIATLELQGLSEVLPPLPRVRCLDDDRLSALVELRESTTGAR
                     IRLGSADRGDLERCTEQLLERLPDAVVDD"
     gene            complement(4480..5616)
                     /locus_tag="VGB85_19955"
     CDS             complement(4480..5616)
                     /locus_tag="VGB85_19955"
                     /inference="COORDINATES: protein motif:HMM:NF025047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4150 domain-containing protein"
                     /protein_id="HEY0136373.1"
                     /translation="MSSVFANGRSILHSGDGLKHVAAPPDVCKTPSPGGPVPIPYVNL
                     ASDSDLSRGTKGVKIEGSSVALADSHLRTSTGDEPGTAGGGIISAKTKGKMGWGTSSA
                     DVRFEGKGVARFMDITRHNGNSYNDAFKARGGTGLAYADDFQGPCPICKKGPREHRIP
                     EKLRSHDLCKRIVDGLKAAYADPARKKGVVKRGYGYMVGVMICKCGQTFAATSGWSPS
                     GFQTVAADVVDEVIQQSSVSLDELIRANKTKKAAPATKRQKLSKAWQEVEDKRAVLTA
                     DAGYNVPGSCAAAKLLTRGHAPLELTEMMFWPKGTWSQEYDLLTTKDPPPRTHSWRKA
                     ILQNRRATKVKSKFSSAANPPTGVASCHTCQTLLYMTNCPKRSC"
BASE COUNT          876 a         2019 c         1994 g          964 t
ORIGIN      
        1 gcgcgaacgc tgcactggtc ctcgatgcat ggatttgccg cgaccggttc ggcgccgccg
       61 acgaacagga cgcccacaat cgccaggagc cccgcctgaa gcacagcgcg ccgccatgat
      121 ccggtttgcc gcatcacgat aatatccacc cgcacgaccc gcgcgtatac ccccgacccc
      181 gtgcgcccgc gctccggcgc gctccccgag gacggaccca atgtgccgcg ctcacggagc
      241 gaccgccacc tcgagatcga tcgccaccgc cgcgacgccg acgatcgtgc ggtcggcggc
      301 ctcggcggtc gtcgacaccg cgcgccgcag cccgccgagc ccggaccagc cggggggcag
      361 cagcgtgacg acctcggcgg gcacgacgaa cgacccgtcg tcggcgatct tgcacacgag
      421 ctcgaaccat gtggtgtggc cctcgggttc gacgaactgc gccctcagct gcagcggcgc
      481 cgcgccggtg ccggtccacg cgagctcgaa cgggccgtcg ccgaaggtcc acgagacccc
      541 ggccggcgcg gtgatcttca gctcggccgg cagcgtgacg aattgcggca actcgcccgc
      601 gggcaggcgc tcgccggccc acgtgattcc gtacgcctcg ccccagcccg ccgggtggcc
      661 cgacccgagg atatcggcgt catagtattt ctgcggcgtc atccccatca ccatcggctc
      721 ggtccagccc gggccgagca gcgtcgccga gccggccgag cgcggcagga cctcggcgcc
      781 gccccagccg aggccgccat accagcgcac gccgcagtcg tcgatccccg tcagcgggtc
      841 gacctcgacc gcgcacatca ccgactcgct cgcctccggc acgtcgaaga attcggcgaa
      901 cgcggagacc tgccattgtg cgtcctccgc ccaccgactg gccgtcactg aaaagcgccc
      961 gccgaactcg ccgccgtacg catcgacctc gtcggggtcg gccggaccga gcccgaccag
     1021 cggccccggc ggctggtcct cgcccgtgcc gccgcagacg gggtcgctgc cggtcgggtc
     1081 gggcgcgccg gtcgtcgagg tcgaggtcga tccggggtcg ctgtcgctcg cggggccggg
     1141 tccgcccgtc gtcgggtcct cggcgtcggt ggtcgtcgac gtggcggcgc ccgtggtgcc
     1201 gtcggcgtga ccggtgtcgc tgttaccgtc ggtgccggtc gtggcggcag tgtcgtcgcc
     1261 gccgccgggc gccttctcgg gctgacaggc ggtgagctgc agggcgaggc acacggccga
     1321 tacattcgtg agacgaggtg agctcatgtg gcgccaagct atcacgcgcg cgcgaagccc
     1381 gaggcgtccg cagcgactcg ccagcggcgc accatgagct cgtgcgttgg ccaggcgcta
     1441 gcgtgcgggt gtcgctcgag gagcagtcgc gcggcgtcgt actcgaacgc gcgcccacgt
     1501 acgaccgttc tctcctcgcc tggttaccgg agctctcgcc tgagatacac tgggccatgg
     1561 tcgccacgcc acgccacgcc acgccgtgct cgcagccgac ggtgacttta taccgcctcc
     1621 ccaagttgcc ggacaggact gaagacgctg ctatggaggc gagctcgacg ccatgagcga
     1681 actggagaac ctgactggat tcgcggcaac atgcctgccg tcgatgttgc cggacgaccg
     1741 gcagttgacg ctagtggtcg tgtcgggccg cttcgtcctg ccgcgtccgg acgggcgcga
     1801 ggcgccgcgg ccgtgcgagg agcagggcga ggtccggctg gccgacgagt acgccgggga
     1861 gccggacgcg ctgctgctgc tccatgaggg gcagagcgcg cccacgcgtt cgggaaccga
     1921 tctgaccatg cttggtcacg cttgggcgcc gggtgggaag cttgccacgc gtagcgatgt
     1981 cgagctgcag gttggccggt gtcacaagcg tgcgctcgtg ttcggagatc gcgtatggtg
     2041 taggacgatg gctggtgtcg cgccgtcgcg ccctgcggcc ttcgagtcga tcccgctgag
     2101 ttatcggcgg tgcttcggcg gcacccccga gcagccatcc acgtcggtcg ccgaggccgc
     2161 ggagtacaac cccgtcgggc ttggcctgca cgagagtgag cgcgaggcga tcgatcgccc
     2221 cgtgccgaac ttcgaagatc ctcaatcacc attgcagcga ccgacaggcc gcccgcgtcc
     2281 ggccggcttc ggcccgatcg cgcggcattg gcgaccgcgg cggacgtacg caggaaccta
     2341 cgatcaggcg tgggtggagc accgcgcgcc actgtggccc cgcgatctcg acgagcgctt
     2401 cttctccgcc gcaccaccgg gcctcttcgc ggtgccgcac ctccaggggg gggagccggt
     2461 gcggatcgcc gggatgtcgc cgcacggcga ccacgcgttc tcgctgcccc gcgtacgcct
     2521 gcgggcgcgc ttcgagctcg ccgatcgttc gatccgcagg gacctggtgc tcgacgccgt
     2581 gcacttcgag ctcgatggct cgatcttcac gatgtactgg cggaccgcga tcgctaccga
     2641 tcctctgaga gtgcgcgcgg tggtcgtacg gaccctcgat ctgtgggagc aagaacggtg
     2701 atacgtatgg tcgtatcaat cgtcggtgtg ggcttggcct gtccggtcgg cctgacctca
     2761 aagacagcca ctgcggcgat ccgggccggg atctcacggt tccaggagat cgacgaggtc
     2821 gtcgggccgg gtgggccggt gcgtgcctcg atgctggcgc aactcggggc cgaccgctcg
     2881 cggaccgagc gcgcgacctt cttcgcccgt cacgcgctgt gcgaggcagt cggcgggctc
     2941 ggcacgatcg acgcgctgcc gtgcttcttc gccttgcccg aacccgacct cgggccgcgc
     3001 atcaatctcg aggatctgct gcgcggcctc gctgacgtcc cgacccgcgc agggagcccg
     3061 ttgcggctct ccctccgccc cgaggccgtc gtgacggctg ggcgtgcggg cgtgttcgct
     3121 gcactcgaga aggccatagc gacgttcgtc gacggcggag cgacgacggc cctgattggt
     3181 gggttcgaca cgctcgtcga tcctgagacg cttgatgccc tcgccgaaca agaccgcctc
     3241 ctcgggcgca ccaacctcga cgggatcatc cccggcgagg cggcggcgtt catcgtcctt
     3301 tccgctctcg acgcgccaag ccgccgatcc gctctggctc ggatctctgt ctcggcgacc
     3361 gcacacgagc cgctgtcgtt cgctcgcgcg aatgatcgcg ccagcgcgtc ggacggcctg
     3421 acgagcgtat ttcgctcgct ccgggcggcg gtcgatggcc gggccgaccg cgtgtacgcc
     3481 ggcgtgaccg gcgagggcta cttcggccaa gagttcgccc acgcgagtct gcgcaacgcg
     3541 gcattgatgc ctgaaccgct gcgctgcgag ccggtcagcg acgcgctcgg cgacatcggc
     3601 gcggctgctg gcgcggtgtg cctcgtgcag gcggtccgcg ccgccgcggg tcagcggagt
     3661 ctcgtctacg cgtcttccga cggcgggcta gtcggcggtt gcgtcgtcga gcccacgcgt
     3721 tgagtcgcgg acacgtcagt cgtcgacgac ggcgtcggga aggcgctcga gcagttgctc
     3781 tgtgcaccgc tcgaggtcgc cacggtcagc cgaccccagc ctgatgcgag cgccagtggt
     3841 cgactcgcgt agctccacga gagcgctgag gcgatcgtcg tcgaggcacc gcacccgcgg
     3901 aaggggcgga agcacctccg agagaccctg gagctccaac gtcgcaattg ccagcttcat
     3961 ggcggagcgg gcctcggccg aggaagcggg caatagcctc agcgtgcgcg tgaaggcgcg
     4021 ccggtcgacc tcgaagacgt cgaacgctcg ccgcgcgatc tgctcgccca gcgtgaagcc
     4081 gacctcctgc actgcctgcg gcacgaactc atccatctcc tcgaacgaga ccagcggcgc
     4141 gtcgataccg tcggaccgtt gcaggtcgag gaagaaatcg aagcgcgtga cggccatcgg
     4201 cgagctctcg aaagcgacct tgaaatatcg gtccggatag ccatccgggg agaagctctc
     4261 aagcagttcg tagaaatcac aagactgaga gggcccgaag gggtggaagt caccgcattc
     4321 gtcgcccatg gcgaccagga agtcggtgta cgtacggggg aacgacaggg agcagttctt
     4381 ctcgcagaga cgtatgtcgt cgagcgatac tccgcggagc gaagcactat acatgggtgc
     4441 acgggccgcg aagaacttca gcaggaatgg cacgatcatt tagcacgatc tcttgggaca
     4501 gttcgtcatg tagagcaggg tttgacaggt atggcaagag gcgacgcccg tgggagggtt
     4561 ggcggcgctg ctaaacttgc tcttgacctt ggtcgcccgc ctgttctgaa gaatggcctt
     4621 ccgccaggaa tgcgtcctgg gaggcggatc cttggtggta agcagatcat actcctgcga
     4681 ccaggtgccc ttgggccaga acatcatctc cgtgagctcg agcggcgcgt gccctctggt
     4741 gagcagtttg gctgccgcac acgagcccgg tacgttgtag ccagcgtcgg cggtgagcac
     4801 ggcgcgcttg tcctcgacct cctgccacgc cttgctgagc ttctgccttt tggtcgcggg
     4861 agccgctttc ttcgtcttgt tggcgcggat cagctcgtcg aggctcacgc tgctctgttg
     4921 gatcacctcg tcgacgacat cggcggcgac ggtctgaaat cccgacgggg accaccccga
     4981 ggtcgcagcg aaggtctgcc cgcacttgca gatcatcaca cctaccatgt agccgtaacc
     5041 ccgcttgacc acgccttttt tgcgcgcagg atcggcgtat gccgctttga ggccatcgac
     5101 gatccgcttg cacaggtcat ggcttcgtaa cttctcgggg atccgatgct cccggggacc
     5161 cttcttgcag atcgggcacg ggccctggaa gtcatcggcg tacgccaggc ctgtgccgcc
     5221 cctcgccttg aaggcgtcgt tgtaagaatt gccattgtgc cgcgtgatgt ccatgaagcg
     5281 cgcgacaccc ttgccttcga agcggacgtc ggcgctgctg gtgccccagc ccatcttgcc
     5341 cttggtcttg gcggaaataa tgccgcctcc tgccgtgccg ggctcatcgc cggtgctcgt
     5401 gcgcaggtgc gagtcggcga gcgcgaccga gcttccctct atcttcacgc ctttggtccc
     5461 tcgtgaaagg tcggagtcgg aggccagatt tacgtacggg atcggcactg gcccgccggg
     5521 agacggtgtc ttgcagacgt cgggcggcgc cgccacgtgc ttcaagccgt cgcctgagtg
     5581 gaggatcgag cgtccgttgg caaataccga ggacatgggg tcagggtatc ggttgaccgg
     5641 atttggtcaa gcacgccgag atgcccgcgc ggcgtcgcgc taccccgaac taccctcgcc
     5701 tcgctcgacg atcagcagca gaacctgtcg acgccgacgg tcccgctcga gcacccgccg
     5761 ccgccgccgc gcgtggcggg gccgtccggt ccgtcgaccc aaccgtgacc gccgctacca
     5821 cggcgcgcag gcgtcgcgcg ggcgcatggg cgt
//
LOCUS       DASWNJ010001183         1695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_480162, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001183 DASWNJ010000000
VERSION     DASWNJ010001183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480162"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1457
                     /locus_tag="VGB85_19960"
     CDS             <1..1457
                     /locus_tag="VGB85_19960"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEY0136374.1"
                     /translation="AGQIDEVRLYGAALSPAQVKSLTRTVYHVARTGLDTNPGTKARP
                     FRTLARGASMAQVGDTVLARAGVYDVGFDEPVVKLDNIGTADEHILFRAYPGESVKLD
                     GGKAPADTDLMQVRGRFIDIVGLELANASKTAINLYRWEGQGGRDIWVQDNEIHHSWR
                     GAVYPNADSTGLHFIGNDVHHNVRVNETKSYCHNGGWPSAVNLTGAGDVVVGNTIHEN
                     WGEGIGAYGSDHVVTDNVLHDNYGVEIYVNNIEDSEIARNFMYTLGIETFKRYFYPDG
                     QGCPLPPAQCTCSLVNKVAAPAVGIGLANEAPGATVLRNNEVVNNIVIGARRSGIQLT
                     SWSSSGPLDMNTTRVAHNTVVVAAESEAFRLWGASTPASDGEVGTIGNNIFVQVRPDR
                     AVAKVMHKVDLVFTHNNWFGGNAAVPALASGPGDVLADPEFSGVGPAPEYYQLGAGSP
                     NIDAGAALGVANDFWGDPRPLGQGPDIGADEYAD"
BASE COUNT          287 a          586 c          579 g          243 t
ORIGIN      
        1 tcgccgggca gatcgacgag gtgcggctgt acggcgcggc gctgtcgccc gcgcaggtca
       61 agtcgctgac gcgcaccgtc tatcacgtgg cgcgcaccgg cctcgacacc aacccgggca
      121 ccaaggcgcg gccgtttcgc acgctcgcca ggggcgcatc gatggcccag gtcggcgaca
      181 ccgtgctcgc gcgcgcgggc gtgtacgacg tcggcttcga cgagccggtg gtcaagctcg
      241 acaacatcgg caccgcggac gagcacatcc tgttccgcgc ctacccgggc gagtcggtga
      301 agctcgacgg cggcaaggcg ccggccgaca ccgacctgat gcaggtgcgc gggcgcttca
      361 tcgacatcgt cgggctcgag ctggcgaacg cgtcgaagac ggcgatcaac ctgtatcgct
      421 gggaggggca gggcgggcgc gacatctggg tgcaggacaa cgagatccac cacagctggc
      481 gcggcgcggt ctacccgaac gccgactcga ccggcctgca cttcatcggc aacgacgtcc
      541 accacaacgt gcgcgtcaac gagaccaaga gctactgtca caacggcggc tggccgtcgg
      601 cggtcaacct gacgggcgcg ggcgacgtcg tcgtcggcaa cacgatccac gagaactggg
      661 gcgagggcat cggcgcctac ggcagcgacc acgtggtcac cgacaacgtg ctgcacgaca
      721 attacggcgt cgagatctac gtcaacaaca tcgaggactc ggagatcgcg cgcaacttca
      781 tgtatacgct cgggatcgag accttcaagc gctacttcta ccccgacggc cagggctgcc
      841 cgttgccgcc ggcgcagtgc acctgctcgc tggtcaacaa ggtcgcggcg ccggcggtcg
      901 gcatcggcct cgccaacgag gccccgggcg cgacggtgct gcgcaacaac gaggtcgtca
      961 acaacatcgt gatcggcgcg cggcgctcgg gcattcagct gacctcgtgg tcgagctcgg
     1021 gtccgctcga catgaacacc acgcgggtcg cccacaacac ggtcgtcgtc gcggcggagt
     1081 cggaggcgtt tcgcctgtgg ggcgcgagca cgcccgcgag cgacggcgag gtcggcacga
     1141 tcgggaacaa tatcttcgtg caggtgcgcc ccgaccgggc ggtcgccaag gtgatgcaca
     1201 aggtcgacct cgtatttacc cacaacaact ggttcggcgg caacgcggcg gtgccggccc
     1261 tggcgagcgg ccccggcgac gtgctggccg acccggaatt ctcgggcgtg gggccggctc
     1321 ccgagtacta ccagctcggc gccggctcgc cgaacatcga cgccggggcg gcgctcggcg
     1381 tggcgaacga cttctggggt gacccgcgtc cgctcggcca gggcccggac atcggcgccg
     1441 acgagtacgc cgactgatcg acgagcatca ctgcttacat accgacgacg ctgacaagcg
     1501 cacttgtcac tggatcttca ccgccctcgg cctggtcggg ctacggctgc gacgacgtcg
     1561 ccgtccgcct gctcaactgc ttctttcgcg tcgagctcgc gcacccgcat attcctgggg
     1621 cgcgttctcg gcccgaggtg ggatcaccca cagcgacatg gccgtgttca acctgccgtg
     1681 agcagggggc ggggc
//
LOCUS       DASWNJ010001184         2904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_514087, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001184 DASWNJ010000000
VERSION     DASWNJ010001184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514087"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VGB85_19965"
     CDS             <1..535
                     /locus_tag="VGB85_19965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136375.1"
                     /translation="LAQQRGAALRNAVPARTIADGQVARPSTRPPNRPPSRAVIHPHE
                     PAPDFTEQGRDDGSAQPERTMILSDASGPPRAEATLMLPSDAPPESTMMLPDDGGRPE
                     STMMLPDDGGHQAEGPSPVHEGTMQLAGEAPAARDGWPLQKILIAINVACGLLILIGL
                     LVLVLGGGADTPPGLEK"
     gene            540..2276
                     /locus_tag="VGB85_19970"
     CDS             540..2276
                     /locus_tag="VGB85_19970"
                     /inference="COORDINATES: protein motif:HMM:NF015376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HEY0136376.1"
                     /translation="MVDRALKVIAPGGEVRTVPLTTAELQVGSSIDSSLLLSGHGVSA
                     KHCRIEQRGDRILVVDRGSKNGTYVNSKRCVEPTELKDGDRIGVGAYTLELAPQRSRN
                     DAARVEARLRGSAVKLARDDGERRAIERERLQRYAREWHGAGRPRRLLLHARDLSTAR
                     GWPEEDRRADPTLAALIEATTRAQRLRLGLSLGLIGAVLAGGMIWWVAVGQPARERAL
                     ATERAQAEEISPAVVAARDVAQPAQPVAPAVKLLEHEVQTGETYEDIANYYGVSPAVL
                     QRFNKIAIGEPAVPGTKLRIPTNKPPRPPLEEERYLVSPGDDWQSIAGFYGLSSTALR
                     RQNPRFGDQLRGGEELRFLVEREPFAAASPNPDDLPIFIVPQGASSEGTVTGGALRNA
                     VQLLPSDLAQVRCASHSYATGYTIKALLQAVAEFRRLGYTGELMIADLSRRDGGQYGP
                     HKSHQSGRDADIWLLPKAKAFKKGCKNCSTDRCRPEPEDIDWATQWRFIQALDAGGLV
                     KEIFLSWRLHEHLHKAAVAQGASPEELKRLIQWPRKPGVPALVMHSEGHVHHIHVRFK
                     CDPSDAACSDLR"
     gene            complement(2281..>2904)
                     /locus_tag="VGB85_19975"
     CDS             complement(2281..>2904)
                     /locus_tag="VGB85_19975"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136377.1"
                     /translation="APHESAVLSLTFSPNSEYLISGGEDARALLRRNREGRLESDAEH
                     PSFAHERRIQGLSFSPDRRWLATGSDDGVISVWNVEAGKTKQRDLTGHEGPIVALAFD
                     ASSEVLVSASQDKTLRLWRVGDLDVGGEVSSVVLRGHGGPITSLRLDPGGRFAISGGE
                     DGAIHVWPLQHELLLRLACRVVGRDLREDEWEELFPGETPAPICERR"
BASE COUNT          420 a         1073 c         1045 g          366 t
ORIGIN      
        1 gctcgcgcag cagcgggggg ccgcgctgcg caacgccgtt ccggcgcgca cgatcgccga
       61 cgggcaggtg gccaggccgt cgacgcgtcc gccgaaccgg ccgccgtcgc gggcggtcat
      121 tcacccgcac gagccggccc ccgacttcac ggagcagggg cgcgacgacg gatcggcgca
      181 gcccgagcgg acgatgatcc tcagcgacgc cagcggcccg cctcgcgccg aggcgacgtt
      241 gatgctgccc agcgacgcgc cgcccgagag cacgatgatg ctgcccgacg acggggggcg
      301 acccgagagc acgatgatgc tgcccgacga cggggggcac caggccgagg ggccgtcgcc
      361 ggtccacgag ggcacgatgc agctcgcggg cgaggccccg gccgcgcgcg acggctggcc
      421 gctgcagaag atcctgatcg cgatcaacgt cgcctgcggc ctgctgatct tgatcggcct
      481 gctggtcctc gtgctcggcg gcggcgcgga cacgcccccc ggtctggaga agtgatctcg
      541 tggtcgaccg ggcgctcaag gtcattgcgc cgggcggcga ggtccgcact gtcccgctga
      601 cgaccgcgga gctgcaggtc ggcagctcga tcgactcgtc gctgctgctc tccgggcacg
      661 gggtctcggc gaagcactgc cggatcgagc agcgcggcga tcgcatcctc gtcgtcgacc
      721 gcggctccaa gaacggcacc tacgtcaact cgaagcgctg cgtcgagccg accgagctca
      781 aggacggcga ccgcatcggc gtgggcgcct acaccctcga gctcgcgccg cagcgctcgc
      841 gcaacgacgc cgcgcgggtc gaggccaggc tgcgcggctc ggccgtcaag ctcgcgcgcg
      901 acgacggcga gcgccgggcg atcgagcgcg aacgactgca acgctacgcc cgcgagtggc
      961 acggcgccgg ccgtccgcgc cgcctgctgc tgcacgcgcg cgacctctcg accgcgcgcg
     1021 gctggcccga ggaggaccgt cgcgccgacc cgaccctggc cgcgctgatc gaggcgacga
     1081 cccgggccca gcgcctgcgc ctggggctga gcctcggcct gatcggcgcc gtgctggccg
     1141 ggggcatgat ctggtgggtc gcggtcggcc agccggcgcg cgagcgagca ctggcgaccg
     1201 agcgggcgca ggccgaggag atctccccgg cggtcgtcgc cgcgagggac gtcgcacagc
     1261 cggcgcagcc ggtggccccg gccgtcaagc tgctcgagca cgaggtccag accggcgaga
     1321 cctacgagga catcgccaac tactacggcg tgtcgccggc ggtgctgcag cgcttcaaca
     1381 agatcgcgat cggtgagccg gcggtgcctg gcaccaagct gcggatcccg accaacaagc
     1441 ccccgcgtcc gccgctcgag gaggagcgct acctcgtcag cccgggcgac gactggcagt
     1501 cgatcgccgg cttctacggg ctgtcgtcga cggcgctgcg ccgccagaac ccgcgcttcg
     1561 gcgatcagct gcgcggcggc gaggagctgc ggtttttggt cgagcgcgag cccttcgccg
     1621 cggcctcgcc caaccccgac gacctgccga tcttcatcgt gccgcagggc gcgagctcgg
     1681 agggcaccgt caccggcggc gcgctgcgca acgccgtgca gctgctgccc tcggatctgg
     1741 cccaggtgcg ctgcgcgtcg cactcgtacg cgaccggcta cacgatcaag gcgctgctgc
     1801 aggcggtcgc cgaatttcgt cgtctggggt acaccggcga gctgatgatc gccgacctca
     1861 gccgccgcga cggcggtcag tacgggccgc acaagtcgca ccagtccggc cgcgacgccg
     1921 acatctggct gctaccgaag gccaaggcct tcaagaaggg ctgcaagaac tgtagcaccg
     1981 accgctgtcg ccccgagccg gaggacatcg actgggcgac gcagtggcgc ttcatccagg
     2041 cgctcgacgc cggcggcctc gtcaaggaga tcttcctgtc gtggcgcctg cacgagcacc
     2101 tgcacaaggc cgcggtcgcg cagggcgcct cgccggaaga attgaagcgg ctgatccagt
     2161 ggccgcgcaa gcccggcgtg ccggcgctgg tgatgcactc ggaggggcac gtgcatcaca
     2221 tccacgtgcg cttcaagtgc gacccgagcg acgccgcctg ctccgacctg cgctgaccgc
     2281 ctagcggcgc tcgcagatcg gcgcgggggt ctcgccgggg aacagctcct cccactcgtc
     2341 ctcgcgcagg tcgcgaccga cgacgcggca ggcgaggcgc agcagcagct cgtgctgcag
     2401 cggccacacg tggatcgcac cgtcctcgcc gccggagatc gcaaagcggc cgcccgggtc
     2461 gaggcgcagc gaggtgatcg gcccgccgtg gccgcgcagc accaccgagc tgacctcacc
     2521 gccgacgtcg aggtcgccga cccgccacag gcgcagggtc ttgtcctggc tggcggacac
     2581 cagcacctcg ctgctcgcgt cgaaggcgag cgcgacgatc ggcccctcgt ggccggtcag
     2641 gtcgcgctgc ttggtcttgc cggcctcgac gttccacacg ctgatcacgc cgtcgtcgct
     2701 gccggtcgcc agccaccggc gatcggggct gaagctcagg ccctggatcc ggcgctcgtg
     2761 ggcgaacgac gggtgctcgg cgtcggactc gaggcggccc tcacggttcc tccgcagcag
     2821 cgcgcgcgca tcctcgccgc cggagatcag gtactcgctg ttggggctaa aggtcagcga
     2881 cagcaccgcc gactcgtgcg gcgc
//
LOCUS       DASWNJ010001185         1585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_91329, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001185 DASWNJ010000000
VERSION     DASWNJ010001185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91329"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VGB85_19980"
     CDS             <1..233
                     /locus_tag="VGB85_19980"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY0136378.1"
                     /translation="DRWTADWQHVAPPGGETVAQLEARVRGWWSALCREHTHLLLAHA
                     GVIRALQVIAGADWPTAMATPIDHLAPHRFTL"
     gene            complement(271..708)
                     /locus_tag="VGB85_19985"
     CDS             complement(271..708)
                     /locus_tag="VGB85_19985"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY0136379.1"
                     /translation="MKFMVMVMVPTSSESEAGVLPDAKELEAMDKFGEEMVTAGVMLA
                     AEGLQPTSRGARIAFSGGTTTVVDGPFTESKELIAGFSILKAKSREEVVAMMRRAPFA
                     EGVTLEVRQLHDPADFASLDPTGELLAKDAVLREQMLANSKLP"
     gene            1055..>1585
                     /locus_tag="VGB85_19990"
     CDS             1055..>1585
                     /locus_tag="VGB85_19990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136380.1"
                     /translation="MNPATALLLPLLASGCLVVNPVFMLDEGTTSVAQTSVQDPLTTD
                     APPTSDGSGSATTGTTSMGGTVGGTTGDLTTGGATSMDTSTTGDPLDSGTDTSTSTST
                     STDTDTDTGTSGGSPDGLPDCDPGDPALIACYDFEPDPNDPTRLVDGSQFDNDGVLSN
                     VGVIAGVEGQAVEILEN"
BASE COUNT          243 a          585 c          529 g          228 t
ORIGIN      
        1 tcgaccgctg gaccgccgac tggcagcacg tcgcgccgcc cggcggcgag accgtcgcgc
       61 agttagaggc gcgcgtgcgc ggctggtgga gcgccctgtg ccgcgagcac acccacctgc
      121 tgctcgccca cgccggcgtg atccgtgccc tgcaggtgat cgctggcgcc gactggccga
      181 ccgcgatggc gaccccgatc gaccacctcg ccccgcaccg cttcaccctg taacagggga
      241 cctgtccttc gggccaggcg cgtccgcgga tcacggcagc ttgctgttgg ccagcatctg
      301 ctcgcgcagc acggcgtcct tggcgagcag ctcgccggtc gggtcgagcg acgcgaagtc
      361 ggcggggtcg tggagctgcc ggacctcgag cgtcacgccc tcggcgaacg gcgcccgccg
      421 catcatggcg accacctcct ccctcgactt cgccttgagg atcgagaagc ccgcgatcag
      481 ctccttcgac tcggtgaagg ggccgtcgac caccgtggtc gtgccacccg agaacgcgat
      541 gcgcgcgccc ctcgacgtcg gctggagccc ctcggccgcg agcatcacac cggccgtgac
      601 catctcctcg ccgaacttat ccatcgcctc gagctccttc gcgtcgggca ggacaccggc
      661 ctcggactcg ctggatgtgg ggaccatgac catgaccatg aacttcatcg ttgcttcctc
      721 gggtatgtcg tcgccgggcg ctccatcgtg gggcccggct ccactgcgac gacgaacgag
      781 ccgccgcagg atcgacacgc cccgcgatct ttttttgccg gcccggcgga cgccggtcgg
      841 tgctcgaggt cggcaggact gatcgacggt acgcgtccgg aaattcaggt cccatcctcg
      901 gccggggagc cgggcgcggg gcggcgcggc gggagcccgt cgcgctcgag caatgcctcg
      961 tcgaggtcga atcggtgcat cgacgagcgc caggtcggcc tgcatcacgg ggcacccacg
     1021 agacgggctt tctcggcgtg gtagtctgct cccgatgaac cccgccaccg ccctcctgtt
     1081 gccgctgctg gcgagcggct gcctggtggt caatcccgtg ttcatgctcg acgagggcac
     1141 cacgagcgtc gcccagacca gcgtccagga tccgctcacc accgatgctc caccgaccag
     1201 cgacgggagc gggtctgcca ccaccggcac cacctccatg ggcggcacgg tcggggggac
     1261 gaccggtgac ctgaccacgg gcggtgcgac gtccatggac accagcacga ccggcgatcc
     1321 gctcgacagc ggcacggaca cgagcacgag cacgagcacg agcacggaca cggacacgga
     1381 cacgggcacc tccggggggt cgccggacgg gctgccggac tgcgatccag gcgaccccgc
     1441 gctgatcgcc tgttacgact tcgagcccga cccgaacgac cccacgcgcc tcgtcgacgg
     1501 ctcgcaattc gacaatgacg gcgtattgtc gaacgtcggg gtcatcgccg gcgtcgaggg
     1561 ccaggcggtc gagatcctcg aaaac
//
LOCUS       DASWNJ010001186         2465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_514099, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001186 DASWNJ010000000
VERSION     DASWNJ010001186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514099"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="VGB85_19995"
     CDS             <1..1138
                     /locus_tag="VGB85_19995"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0136381.1"
                     /translation="KPIREARAEVRGVAARIESFIGQAQAACAEETATEGGIRMKVRW
                     RPLGVVAVIGPWNYPVATPNNLVVSALLTGNAVVFKPSEFTPHTGALYHALLSRHLAG
                     GLVGLAQGTGQVGAALVAGDVDMIAFTGSIATGQAIMRTAAATMKRLVLELGGKDPMI
                     VLPGADLEAAAKHAATEATRNAGQVCVAVERIIVHQDIAEAFSARVAEIVRGLAVGDP
                     RDEATQVGPMANARQRDLVLGQLARARAAGARFLVEGESRGPGFYLTPSVVVGVTPEM
                     DLAREETFGPVVAIQVARDADEAVARANDTVYGLGASVWGPPGAETEAIADRLEVGMV
                     GVNRGLSTAGGAPWVGWKMSGFGYTRSVAGMRQFMQPQSRASRA"
     gene            1232..>2465
                     /locus_tag="VGB85_20000"
     CDS             1232..>2465
                     /locus_tag="VGB85_20000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136382.1"
                     /translation="MGLLSTLLHEASARRPAEVVIETDRPVRMRLADGTSAELAAAVQ
                     GTAISDELSDLLTTEQQVDLALGEPVKFDLVVGLYTWHLVGETAQEVVSVRACPTGEL
                     LEASDSVQIPIEPDDVDLESEPEDIPRIRRAPSSGPSEIDIDINFDDSFEDEAASPTT
                     QAPDDRPPAGLRLAALKPVPGDSKPQPVWPRRVARGTPERGARSPGARTAATARAPDT
                     ATNWPRDGRRGRTPPASTQGTQGTQGPQTTNTRQLPLRPAPRREDMAELLAAFEPGSL
                     ALLLRAPGQAEALAAALELPSLTLGESDTPAQASARLGELGHHGQHGHHGHHGHGNGG
                     VLIVRAEDPSPWLAWLLRRVEEGRRVIVETDALTPAGARRILLGVGATPRAELWLDAV
                     RVLSATVSEDRWILLSPGN"
BASE COUNT          328 a          925 c          916 g          296 t
ORIGIN      
        1 caagccgatc cgcgaggcgc gggcggaggt ccgcggggtc gcggcgcgca tcgagtcgtt
       61 catcggccag gcgcaggccg cctgcgccga ggagaccgcg accgagggcg ggatccggat
      121 gaaggtccgc tggcggccgc tcggcgtggt cgcggtgatc gggccgtgga actaccccgt
      181 ggcgacgccg aacaacctcg tcgtgtcggc gctgctgacc ggcaacgcgg tcgtgttcaa
      241 gccctcggaa ttcacgccgc acaccggcgc gctgtaccac gccctcctgt cacggcacct
      301 ggccggcggg ctcgtcggcc tggcccaggg gacaggtcag gtcggcgcgg cgctcgtcgc
      361 cggcgacgtc gacatgatcg cgttcaccgg atcgatcgcc accggtcagg cgatcatgcg
      421 caccgcggcg gcgacgatga agcgcctcgt gctcgagctc ggcggcaagg acccgatgat
      481 cgtgctgccg ggcgccgacc tcgaggcggc ggcgaagcac gcggcgaccg aggcgacccg
      541 caacgccggc caggtgtgcg tcgcggtcga gcggatcatc gtgcaccagg acatcgccga
      601 ggcgttctcg gcccgcgtgg cagagatcgt gcgcggcctg gcggtcggcg atccccgtga
      661 cgaggcgacg caggtcgggc cgatggccaa cgcccgccag cgtgacctgg tgctcgggca
      721 gctcgcccgg gcccgggccg ccggcgcccg cttcctcgtc gagggcgagt cgcgcggccc
      781 gggcttctat ttgaccccca gcgtcgtcgt cggcgtcacc cccgagatgg acctcgcgcg
      841 cgaagagacc ttcgggccgg tcgtcgccat ccaggtcgcc cgcgacgccg acgaggcggt
      901 cgcccgcgcc aacgacaccg tctacggcct cggcgccagc gtgtggggcc cgccgggcgc
      961 cgagaccgag gcgatcgccg accggctcga ggtcggcatg gtcggcgtca atcgcggcct
     1021 gagcaccgcg ggtggggccc cgtgggtcgg ctggaaaatg tccggcttcg gctacacccg
     1081 gtccgtcgcc ggcatgcggc aattcatgca gccgcagtcg cgggcctccc gcgcctgagc
     1141 cccggatccc cggtagggcg cactggagtg tcgccgggcc ggccctgatc atctccgccc
     1201 aaccggcgcg cgggtttgtt atggtaccgc cgtggggctc ctttccacgc tgctgcacga
     1261 ggcgagcgca cgtcgcccgg ccgaggtcgt catcgagacc gatcggccgg tccgcatgcg
     1321 cctggccgac ggcacgagcg cggagctggc cgcggcggtg cagggcaccg cgatctcgga
     1381 cgagctgtcg gacctgctga ccaccgagca gcaggtcgac ctcgcgctcg gcgagccggt
     1441 gaagttcgac ctcgtcgtcg gcctctacac ctggcacctc gtcggcgaga ccgcccagga
     1501 ggtcgtgtcg gtgcgcgcct gcccgaccgg cgagctgctc gaggccagcg acagcgtcca
     1561 gatcccgatc gagcccgacg acgtcgacct cgagagcgag ccggaggaca tcccgcggat
     1621 ccgtcgcgcg ccctcgtcgg gcccgtcgga gatcgatatc gacatcaact tcgacgacag
     1681 cttcgaggac gaggccgcga gcccgacgac gcaggccccc gacgaccgac cgcccgcggg
     1741 cctgcgcctc gccgcgctca aacctgtccc cggagacagc aagccgcagc cggtgtggcc
     1801 gcggcgggtc gcccggggca cgccggagcg aggggcgcgg agccccggcg cacgcacggc
     1861 cgcgacggcg cgcgctccgg acaccgccac gaactggccg cgcgacggcc ggcgtggccg
     1921 cacgccgccg gcgtccacgc aaggcacaca aggcacacag ggcccgcaga ccacgaacac
     1981 ccgccagctg ccgctgcgcc cggcgccgcg ccgcgaggac atggccgagc tgctcgccgc
     2041 gttcgagccc ggcagcctgg cgctgctgct gcgcgcgccg ggtcaggccg aggcgctggc
     2101 ggcggcgctc gagctgccgt cgttgacgct cggcgagtcc gacacgccgg cgcaggcgag
     2161 cgcgcgtctc ggcgagctcg gccaccatgg ccagcacggc caccatggcc accacggcca
     2221 cggcaatggc ggcgtgctga tcgtccgcgc cgaggacccg agcccgtggc tcgcgtggct
     2281 gctgcgccgc gtcgaggagg gccgccgggt gatcgtcgag accgacgcgc tgacgcccgc
     2341 gggcgcgcgg cggatcctgc tcggcgtcgg cgccacgccg cgcgcggagc tgtggctcga
     2401 cgcggtccgg gtgctcagcg cgaccgtcag cgaggatcgc tggatcctgc tgtcgcccgg
     2461 caact
//
LOCUS       DASWNJ010001187         1883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_270084, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001187 DASWNJ010000000
VERSION     DASWNJ010001187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270084"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            470..685
                     /locus_tag="VGB85_20005"
     CDS             470..685
                     /locus_tag="VGB85_20005"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0136383.1"
                     /translation="MRSRREAELPPPLLTVRDLAVLLATSSSGVYSRVARGQIPGVLR
                     IGRSLRFDPRVIAQWLSANKASAGGLS"
     gene            682..1746
                     /locus_tag="VGB85_20010"
     CDS             682..1746
                     /locus_tag="VGB85_20010"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY0136384.1"
                     /translation="MIKLRPWKDTKNEFEADIIVNGPNGRTLRKRVKAPVTGKSNAER
                     WARAIEGELLAQLLAPEPEPEKPPAPTFEEFAVTFLGLCRSNRLGVNTLTNYEWHLRL
                     YLVPVLGKRRLDEAKPSDITAIKDSLSAKARNTMCEVLKTLRRVFNAAIDEKILEHAP
                     VKFEIPARVHKAPVAYDEADQAALLAAAQVLGPDYVAMVLLGIDGGLRRGEILGLQWS
                     DINIKRATLTIRHNVVRGKLDVPKGRTEDDVSMTKRLGEALAAARHQRGPFVFSTERG
                     EHYKEHNIERWMEQLVEASKVPWRATHVLRKTCGTRIADGGGGVAAIAAHLRHKDMQT
                     ANRYIDRNGASSRAINALES"
BASE COUNT          367 a          629 c          604 g          283 t
ORIGIN      
        1 gaagggatta ctgcaaccac tgtggaaaga cctgctgcaa tctctgcgga agagattgct
       61 gcaaccactg cggaaagttc gttgccacca ttgcagcatt attttccgaa agtacgacac
      121 tatccatcgc ccgcggcctg acggacagtc gaggcgtggg cgatacagaa gctgatcttc
      181 gaggtgctca ggccagacca agcagccggc gttgcagaga gtcctgtact tcgactttcg
      241 ctgaatctgg ccgaggccgg ttggcggcac ggctcgagcg gagcgcgacg gtctccgccg
      301 cagcaccgcc tgccgtcgcg ccaaatgtcg gcgtcaggtc ggcacggcct gaagaacgag
      361 tggaagggag aagaccgtga aggcgcccgg tcccagggtg gccgcggggc agagccatgg
      421 cctccaagac agggtccgtg cgatcccagt tcgcaccggc cacgaaggcg tgagatcgcg
      481 cagagaggcc gagttgccgc cgccgctgct caccgtccga gaccttgccg tgctgctcgc
      541 cacgagcagt agcggcgttt actcccgggt tgcacgcggc cagatccccg gtgttcttcg
      601 catcggtcga agccttcgct tcgacccgcg ggtcatcgca caatggctct cggcgaacaa
      661 ggcgagcgca ggaggtctct catgatcaag ctgcgcccgt ggaaggacac gaagaatgaa
      721 tttgaagccg acatcatcgt caacggcccc aacggccgga ccttgcgcaa gcgcgtcaag
      781 gcaccggtca cgggcaagtc caacgccgag cggtgggccc gcgcgatcga gggggagttg
      841 ctcgcgcagc tcctcgcccc cgagcccgag cccgagaagc cgcccgcgcc caccttcgag
      901 gagttcgcgg tgaccttcct cggtctatgc cggtccaacc ggctcggcgt gaacacgttg
      961 acgaactacg agtggcacct gcggctctac ctcgtccccg tgctcggcaa gcgacggctc
     1021 gacgaggcca aaccttcgga catcaccgcg atcaaggact cgctctcggc gaaggcgcgc
     1081 aacacgatgt gcgaggtgct caagaccttg cgccgcgtgt tcaacgcggc catcgacgag
     1141 aagatcctcg agcacgcgcc ggtgaagttc gagatcccgg cgcgggtcca caaggcgccg
     1201 gtggcctacg acgaggccga ccaggcggcg ctgctcgccg ccgctcaggt gctcgggccc
     1261 gactacgtcg ccatggtgct acttggcatc gacggcgggc tgcggcgcgg ggagatcctc
     1321 gggctccagt ggtcggacat caacatcaag cgcgccaccc tcaccatccg gcacaacgtc
     1381 gtccgcggca agctcgacgt tcccaaggga aggactgagg acgacgtcag catgacgaag
     1441 cgtctcggcg aagcgctcgc cgccgctcga caccagcgcg gcccgttcgt gttctcgacc
     1501 gagcgcggcg agcactacaa ggagcacaac atcgagcgct ggatggaaca gctcgtcgag
     1561 gccagcaagg tcccgtggcg ggcaacccat gtgctccgca agacctgcgg cacccgcatc
     1621 gcggacgggg gcggcggtgt ggcggccatc gccgcccacc tgcgccacaa ggacatgcag
     1681 accgcgaacc gctacatcga ccgcaacggt gccagttccc gcgcgatcaa cgcgctggag
     1741 agctgatgga ggcgtgatca cgcccgcggc taacttagcg taactataag atcaatcaac
     1801 tagactctca ctgctccacg caataaagcg cccagtcgta ggcgcagtcg gtcgtcggct
     1861 gccctgcgac ctcggtcgag acc
//
LOCUS       DASWNJ010001188         2924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_57536, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001188 DASWNJ010000000
VERSION     DASWNJ010001188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57536"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VGB85_20015"
     CDS             complement(<1..461)
                     /locus_tag="VGB85_20015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136385.1"
                     /translation="MHRMFLGSGLAFGFGLVGSLATLGMQGCGGGDYCAVVFNKQIEC
                     APAEQKALFEGLKDLALKECRDKKDRDADVEKSELECAQKAGCEDFKKCMDALSDAKY
                     AKKVKREIEAALSTGEKLEDALSTCKFSDIKDDGVKKLCGDLFTKALDTATT"
     gene            complement(648..1745)
                     /locus_tag="VGB85_20020"
     CDS             complement(648..1745)
                     /locus_tag="VGB85_20020"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006592.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEY0136386.1"
                     /translation="MTDLADGETFEMQGSGAKPYVLKNTGGVYSCSCPAWRNQSAPPE
                     RRSCKHLKKLRGDAQEQARVGEAASAARPARAPVSSASTDATESEEATAPPLLLAHVW
                     DNETDLAGWWMSEKLDGVRAYWDGQRFLSRLGNPYHAPSWFTAGLPDFPLDGELWLAR
                     KAFQRTVSVVRRQDQSDAWQQIRFLVFDAPGHGGAFEARVAHYHAYIADKKPAFVQAH
                     PHEPCKGVDHLRAELARVEALGGEGLMLRRPGSAYEVGRSHSLLKVKSFFDAEARVTG
                     HQDGAGRHRGRLGALLVELQDGTKFAVGSGLSDAERDDPPPVGSIITFRYQELSDGGV
                     PRFPTYVGVRHDVAWKPGKGPGAVRPAAKRK"
     gene            complement(1803..>2924)
                     /locus_tag="VGB85_20025"
     CDS             complement(1803..>2924)
                     /locus_tag="VGB85_20025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136387.1"
                     /translation="GYFKRRAGVGVRPLHGDAVHFLNPEERRNLRRIERHAVLRAGAA
                     GALAASLGAVISVLARPLLGDEPADASWGETLRYLGVTLLTAVPVAIVELSFIYSNAI
                     VSVHRLAEAAGVALFRPDDASGDEEDEIFAAVLARAALEIPNPPRPLFGIDPRREASR
                     WRLILATVIYKAKVSVSNFVLRKLLVRAFGRAGLRSWIPFVAVPVTATWDAFVCRRAL
                     REARLRAIGPSAARELVDCLLAQVPAPSALLHEAMFRAIATAVVRSVDFHPNLVELLN
                     ALKRRVGAFETDEIDDSRRALAVMQRLEPTERRVALEMLVVACVFDGRIGRKEWAVLG
                     RAYALFGWTTPRAEVLAFKRSLLNGDELGVDQLAGLLPS"
BASE COUNT          419 a         1041 c         1019 g          445 t
ORIGIN      
        1 gtggtcgcgg tgtcgagcgc cttggtgaac aggtcgccgc acagcttctt gacgccgtcg
       61 tccttgatgt cgctaaactt gcaggtcgac agggcgtcct cgagcttctc gccggtggag
      121 agcgccgcct cgatctcccg cttgaccttc ttggcgtact tggcgtcgct gagcgcgtcc
      181 atgcacttct tgaagtcctc acagccggcc ttctgcgcgc actcgagctc ggacttctcg
      241 acgtcggcat cgcggtcctt cttgtcgcga cactccttga gcgcgaggtc cttgaggccc
      301 tcgaacaggg ccttttgctc ggcgggcgcg cactcgatct gcttgttgaa gacgacggcg
      361 cagtagtcac cgccgccgca tccctgcatg cctaaggtgg cgagagagcc gacgagaccg
      421 aagccgaagg cgaggccgga gccgagaaac atgcgatgca tcgtgttcat gggggtcgag
      481 atttccgtgt tgatccgtgg tggcgcccgc agactgcgtc ccggcagcat ggccattcga
      541 gtcgggcgaa ttcgcgccgc gcgtagtacc acgaaatcga ctcccctgta aatctctctg
      601 cgcaccgcgc tgctcgctcg cagtgaacac cgctccgacg gccccggcta tttgcgtttg
      661 gctgccggcc ggaccgcgcc cggccccttg cctggcttcc acgcgacgtc gtgacgcacg
      721 ccgacgtagg tcgggaagcg cggcacgccg ccgtcgctga gctcctgata gcggaacgtg
      781 atgatgctac cgaccggcgg cgggtcgtcg cgctcggcgt cgctgaggcc gctgccgacg
      841 gcgaacttgg tgccgtcctg cagctcgacc agcaacgcgc cgagtcggcc gcgatggcgg
      901 ccggcgccgt cctggtggcc ggtcacgcgc gcctcggcgt cgaagaagct cttgaccttg
      961 agcaggctgt gcgagcgacc gacctcgtag gcggagccgg ggcgacgcag catcaggccc
     1021 tcgccgccga gcgcctcgac gcgcgcgagc tcggcccgca ggtgatcgac gcccttgcat
     1081 ggctcgtgcg ggtgggcctg cacgaacgcc ggcttcttgt cggcgatgta cgcgtggtag
     1141 tgggcgacgc gcgcctcgaa ggcgccgccg tggccgggcg cgtcgaacac gaggaaccgg
     1201 atctgctgcc acgcgtcgct ctggtcctgc cggcgcacga cgctgaccgt ccgctgaaat
     1261 gccttgcgcg cgagccacag ctcgccgtcg agcgggaagt cgggcaggcc cgcggtgaac
     1321 cacgacggcg cgtgatacgg gttgccgagc cgcgacagga agcgctgacc gtcccagtac
     1381 gcgcgcacgc cgtcgagctt ctcgctcatc caccaccctg ccaggtcggt ctcgttgtcc
     1441 cagacgtggg cgagcagcag cggcggcgct gtcgcctcct cgctctcggt cgcgtccgtg
     1501 ctggccgaag agacaggtgc gcgggccggt cgtgcggcgc tcgccgcctc gccgacgcgg
     1561 gcctgctcct gcgcgtcacc gcggagcttc ttgaggtgct tgcagctgcg ccgctcgggc
     1621 ggcgccgact ggttacgcca cgccgggcag ctgcacgagt acacgccgcc ggtgttcttc
     1681 aacacgtagg gcttggcccc ggagccctgc atctcgaagg tctcgccatc cgccagatcc
     1741 gtcatcacca ccacgctcct gttgagccgc aagcgtaggg tgtttgtcgc gtccagggga
     1801 gatcacgagg gcagcagacc cgcgagctga tcgacgccga gctcgtcgcc gttgagcagg
     1861 ctgcgcttga atgcgagcac ttccgcgcgc ggcgtcgtcc acccgaacag cgcgtatgcg
     1921 cgccccagca ccgcccactc cttgcgaccg atccgcccgt cgaacacgca agcgacgacg
     1981 agcatctcca gcgcgacgcg gcgctccgtc ggctcgaggc gctgcatcac cgccagggcg
     2041 cgccgcgagt cgtcgatctc gtcggtctcg aaggcgccga cgcgccgctt gagcgcgttg
     2101 agcagctcga cgaggttcgg gtgaaagtcg acgctgcgga ccacggcggt ggcgatcgcc
     2161 cggaacatcg cctcgtgcag cagggccgac ggcgcgggga cctgcgcgag caggcagtcg
     2221 acgagctcgc gcgccgccga cgggccgatc gcccgcagcc tcgcctcgcg cagggccctg
     2281 cggcacacga aggcgtccca ggtcgcggtg accggcaccg cgacgaaggg gatccagctg
     2341 cgcaggccgg cgcggccgaa ggctcgcacc agcagcttgc gcagcacgaa gttgctgacg
     2401 ctgaccttgg ccttgtagat gaccgtcgcc aggatcaggc gccagcgcga ggcctcgcgc
     2461 cgcgggtcga tgccgaacaa cggccgcggc gggttcggga tctcgagcgc cgcgcgggcg
     2521 agcacggccg cgaagatctc gtcctcctcg tcgccgctgg cgtcgtccgg gcggaacagc
     2581 gcgaccccgg ccgcctccgc gaggcgatgc accgacacga tcgcgttgga gtagatgaac
     2641 gagagctcga cgatcgcgac cgggaccgcg gtcagcagcg tgaccccgag gtagcgcagc
     2701 gtctcgcccc agctggcgtc ggccggctcg tcgccgagca gcgggcgcgc gagcacgctg
     2761 atcaccgcgc cgagcgaggc cgcgagggcc ccggccgcgc ccgctcgtag caccgcgtga
     2821 cgctcgatcc gccgcaggtt ccggcgctcc tcggggttga ggaagtggac cgcgtcgccg
     2881 tgcagcggtc gcacgccgac gcccgcgcgc cgcttgaagt agcc
//
LOCUS       DASWNJ010001189         2424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_232941, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001189 DASWNJ010000000
VERSION     DASWNJ010001189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232941"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(350..667)
                     /locus_tag="VGB85_20030"
     CDS             complement(350..667)
                     /locus_tag="VGB85_20030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136388.1"
                     /translation="MAPRPPESREQFIRRLRAAGDSVAGQPLGFGGHHFTHAVVVEGG
                     RWSVRRLILDQAKVDAYRAEHRVFMPEHAEMLSEPGPEEVLGADSLAGLVAAIEAGEW
                     PLD"
     gene            complement(725..1567)
                     /locus_tag="VGB85_20035"
     CDS             complement(725..1567)
                     /locus_tag="VGB85_20035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0136389.1"
                     /translation="MNAPEDDAQGLKARVREHWDREPCGTRGLDDAAGDDARARQARA
                     EQERYAVDAHIPGFARFVESAGLRVLEIGVGAGTDFVQWLRNGAKATGVDLSPNSLAE
                     CRARVIAEGHPDVSLQVADAEHLPFADASFDLVYSYGVLHHSPNTAAAIGEVLRVLRP
                     GGEARIMIYHVPSWTGLMLWAVHGAARLRPWISPRRAIYEHLESPGTKAYTLAEARAL
                     MRDFEEVELETALLAGDLLAMRPSARYQSPVHRLLWAVYPRGLIKRFGARLGLGLLIR
                     ARKP"
     gene            1615..>2424
                     /locus_tag="VGB85_20040"
     CDS             1615..>2424
                     /locus_tag="VGB85_20040"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEY0136390.1"
                     /translation="MCGIAGMIGRQDPALLAAMTAGLRHRGPDGEGVHVDGPLGLGHR
                     RLSVLDLAGGAQPMASVDGRAVVSFNGEIYNFKQLRRELLAAGKPLHTQGDTEVLLAG
                     YQHWGLHGLLARLRGMFAFALWDLSSGTCVLVRDRLGIKPLYHASLPDGALLFASEPK
                     ALLECAEVDRSLDLVSLDAYLDLFYIPPPRSIFAGVRQLPPGHLLTWRAGHVTIDRWW
                     DAPPRPADPRLRTLDDWAEAIEPVLREAVELHTVSDVPLGAFLSGGLDSSAI"
BASE COUNT          343 a          892 c          828 g          361 t
ORIGIN      
        1 cgtgacaggc cggcgttata accgacttaa ctaccacccc tcccaggtcg acctttttgg
       61 gggccgacag ggaccgggag aatgctgtac gccccccggc tctgtcaaga acgatcgttc
      121 gtgcctcgtc ggatcgttcg caagcggccc cagctggtca ccacggccag aataggcgga
      181 ccctgcccca cgagcgagtc gcggacggct ctcacgacgc gcacccgacc gaccttcgaa
      241 cctgcccatg cgcgggtcca gggagatctc tccgggctca tcatgggccc gcgagtcgtc
      301 gccgacgagc gccggaggtc gatctcgtga gcaaactcga gatcgcaggt caatccagcg
      361 gccactcgcc ggcctcgatc gcggcgacca gcccggcgag cgagtcggcc ccgagcacct
      421 cctcgggtcc gggctcgctg agcatctcgg cgtgctccgg cataaagacc cggtgctccg
      481 cgcgataggc atcgaccttc gcctggtcga ggatcagtcg gcggaccgac cagcgtccgc
      541 cctcgacgac caccgcatgc gtgaagtggt gtcctccaaa gcccagcggt tggccggcga
      601 cgctgtcgcc cgcggcgcgc aggcgccgga tgaattgctc gcgcgactcg ggcggacggg
      661 gagccatccc caaaggatcg ccgacctcgc ggcggcgtac cagaggtccc gcctgtcctt
      721 tcggtcaggg ctttcgcgcg cggatcagca acccgaggcc gaggcgtgcg ccgaagcgct
      781 tgatgagacc tcgcgggtac acggcccaca gcaggcggtg taccgggctc tggtagcgcg
      841 cggagggccg catcgccagc aggtcacctg ccaacagcgc cgtctcgagc tcgacctctt
      901 cgaaatcccg catcagcgcg cgcgcctcgg cgagcgtgta cgccttggta ccggggctct
      961 cgaggtgctc ataaatggcg cgccgcgggc tgatccacgg gcgcaggcgg gcggcgccgt
     1021 gcacggccca cagcatcagg ccggtccacg agggcacgtg gtagatcatg atgcgcgcct
     1081 cgccgccggg tcgcagcacc cgcagcacct cgccgatcgc agccgcggtg ttcggcgagt
     1141 ggtgcaggac cccgtacgag tacacgaggt cgaagctcgc atctgcaaac ggcaggtgct
     1201 cggcgtcggc gacctgcagc gagacatccg ggtgtccctc ggcgatcacg cgggcgcgac
     1261 actcggcgag gctgttgggg ctgaggtcga cgccggtcgc cttcgcgccg ttgcgcagcc
     1321 actgcacgaa gtcggtgccg gcaccgacac cgatctccag cacccgcagg ccggcggact
     1381 cgacgaagcg ggcgaacccg gggatgtgcg cgtcgacggc gtagcgctcc tgctcggccc
     1441 gggcctggcg cgcgcgcgcg tcgtcgccgg ccgcgtcgtc gaggccccgc gtcccgcagg
     1501 gctcgcggtc ccagtgctcg cggacgcggg ccttcagccc ctgcgcgtcg tcttcgggtg
     1561 cgttcatccg ggcgagctta cgcgagttcg gcgggcgcgc taagctcccg gcgcatgtgc
     1621 gggatcgcgg ggatgatcgg ccggcaggac ccggcgttgc tcgcagcgat gaccgccggc
     1681 ttgcggcacc gcgggcccga cggcgagggc gtgcacgtcg acggtccgct cgggctcgga
     1741 caccggcgcc tctccgtcct cgacctcgcc ggtggcgcgc aaccaatggc cagcgtcgac
     1801 ggtcgcgccg tcgtcagctt caacggcgag atctacaatt ttaaacagct gcgccgcgag
     1861 ctgctcgccg ccggcaaacc attgcatacg cagggcgaca ccgaggtgct gctcgccggc
     1921 tatcagcact ggggcctgca cggcctgctc gctcgcctgc gcgggatgtt cgccttcgcc
     1981 ctctgggacc tgtcctccgg gacatgcgtg ctcgtacgcg accgcctcgg gatcaagccg
     2041 ctgtatcatg cgagcctgcc cgacggcgcg ctgctgttcg cctcggagcc caaggcgctg
     2101 ctcgagtgcg ccgaggtcga ccgcagcctc gacctcgtca gcctcgacgc ctacctcgac
     2161 ctgttctaca tcccgccgcc gcggtcgatc ttcgccggcg tccgccagct cccgccgggt
     2221 cacctgctga cctggcgcgc gggacatgtg acgatcgatc gctggtggga cgcgccgcca
     2281 cggcccgccg acccgcgcct gcgcacgctc gacgactggg ccgaggcgat cgagccggtg
     2341 ctgcgcgagg cggtcgagct gcacaccgtc tcggacgtcc cgctcggcgc cttcctgtcc
     2401 ggcggcctcg actcctcggc gatc
//
LOCUS       DASWNJ010001190         2138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_658146, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001190 DASWNJ010000000
VERSION     DASWNJ010001190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658146"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..745
                     /locus_tag="VGB85_20045"
     CDS             209..745
                     /locus_tag="VGB85_20045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136391.1"
                     /translation="MPARPHAARRLPGVLAGLLVGSLMLLACSPYQLGPPPADPLAVP
                     RPFTGYVDAMATVCAIRTARLAMAVTFLVHDNDLLVGATRGPTWFCWRAEPGRHRIVV
                     TSEDGGQRFEVDLVERGRYYLDQGLDYRLGFVTPRARWVDEAAAAALLQTSEHRVLQA
                     APAREPLVLGTDVAAALP"
     gene            complement(753..>2138)
                     /gene="otsB"
                     /locus_tag="VGB85_20050"
     CDS             complement(753..>2138)
                     /gene="otsB"
                     /locus_tag="VGB85_20050"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEY0136392.1"
                     /translation="GEILLLGVDRLDYTKGIRRRLKAVERVLERRPDLRGKLRMVQVA
                     VPSRENVGAYGDFRARVEQTVGAINGKFSTLGNVVVHYLYRGLPQEQLLSLYRAAAVI
                     LVTPLRDGMNLVAKEFAAARTDGDGVLVLSEFAGAAEEMPEALLVNPFDIDGMADAIE
                     QALAMPEAERRTRMTQLNARVRHFDVHRWADNFLEALAEDDPAAHQRQPPLLATQTLL
                     AAVREARRLVVLLDYDGTLVRFAPRPEMAAPDPELLTLLEDLAKRPGCSLHIVSGRPR
                     EDLERWFAGLPVGLHAEHGLFSRVDGDWHARGSLPEDLRSKVRATLERVVQCTPGSRV
                     EEKAVGFAWHYRQAEPEHGASQARELRLHLRELLIGTPLEVLPGDKIVEVRLRGVHKG
                     VVLHGLIGHGELALAIGDDRTDEDLFTTLPETAITVRVGRAASAARYHLPDVTAVRAT
                     LRALVDAASEQ"
BASE COUNT          279 a          890 c          668 g          301 t
ORIGIN      
        1 cgcctcgagc gccgtgaaca cctccttgag cgacatcgcc gccgccacct tgagctcgcg
       61 cggccgctcc cccgcgcacg cgcatcccag cgagatcgtc acgagcaagg tcgagagccg
      121 caccggcgca gcatagcgac gcccccgccg cgcacgcaaa cctgcgattg gcccgcgccc
      181 gaccccgcgg tatacctcgc agggagccat gccagcccga ccccacgcgg cccgccgcct
      241 gcccggtgta ctcgccggcc tgctcgtggg ctccctcatg cttttggcct gctccccgta
      301 ccagctcggc ccgccgcccg ccgacccgct cgccgtcccg cgcccgttca ccggctacgt
      361 cgacgccatg gcgaccgtct gtgcgatccg cacggcccgg ctggcgatgg ccgtcacctt
      421 cctcgtgcac gacaacgacc tgctcgtcgg ggccacccgc ggcccgacct ggttctgctg
      481 gcgcgccgag cccggccgcc accgcatcgt cgtcaccagc gaggacggcg gccagcgctt
      541 cgaggtcgac ctggtcgagc gcggccgcta ctacctcgac cagggccttg actatcgcct
      601 cggcttcgtc acgccgcgcg ctcgctgggt cgacgaggcc gccgccgccg cgctgctgca
      661 gaccagcgag caccgcgtgc ttcaggccgc gcccgcgcgc gagcccctcg tgctcggcac
      721 cgacgtcgcc gccgcgctgc cctgagcccg cgctattgtt cgctcgcggc gtccaccagc
      781 gcccgcagcg tcgcccgcac cgccgtcaca tcgggcaaat gatagcgggc cgcgctcgcc
      841 gctcgtccga cgcgcaccgt gatcgcggtc tccggcagcg tcgtgaacag atcctcatcg
      901 gtgcggtcgt cgccgatcgc cagcgccagc tcgccgtggc cgatcagccc gtgcagcacc
      961 actcccttgt ggaccccacg cagccgcacc tcgacgatct tgtcgcccgg cagcacctcg
     1021 agcggcgtgc cgatcaacag ctcgcgcagg tgcagccgca gctcgcgggc ctggctcgcc
     1081 ccgtgctccg gctccgcctg gcggtagtgc cacgcgaagc cgaccgcctt ctcctcgacc
     1141 cggctgcccg gcgtgcactg gaccacccgc tcgagcgtcg ctcgcacttt gctgcgcagg
     1201 tcctcgggca gcgagccacg cgcgtgccag tcgccgtcga cccgcgagaa cagcccgtgc
     1261 tccgcgtgca gcccgaccgg cagccccgcg aaccaccgct cgaggtcctc gcgcggccgg
     1321 cccgacacga tgtgcaacga acaacctggc cgtttggcca ggtcctccag cagcgtcagc
     1381 agctcggggt ccggcgcggc catctccggc cgcggggcga agcgcaccag ggtgccgtcg
     1441 tagtcgagca gcaccaccag ccgcctcgcc tcgcgcaccg ccgccagcag cgtctgcgtc
     1501 gccagcagcg gcggctgtcg ctggtgcgcc gccgggtcgt cctcggcgag cgcctcgagg
     1561 aagttgtcgg cccagcggtg cacgtcgaag tgtcggacgc gcgcgttgag ctgcgtcatg
     1621 cgcgtgcgtc gctccgcctc cggcatcgcc agcgcctgct cgatcgcgtc ggccatcccg
     1681 tcgatgtcga aggggttgac cagcagcgcc tccggcatct cctccgccgc gcccgcgaac
     1741 tcgctgagca ccagcacccc gtcgccgtcc gtgcgcgccg ccgcgaactc cttggcgacc
     1801 aggttcatcc cgtcgcgcag cggcgtcacc agtatcaccg ccgccgcgcg gtacaggctc
     1861 agcagctgct cctgcggcag tccgcgatac aggtaatgca cgaccacgtt gccgagcgtc
     1921 gagaacttgc cgttgatcgc cccgaccgtc tgttcgacgc gggcacgaaa gtccccgtat
     1981 gcgccgacgt tctcgcgcga cggcaccgcc acctgcacca tccgcaattt gccgcgcagg
     2041 tccggccgcc gctcgagcac ccgctcgacc gccttgagcc ggcgccggat ccccttcgtg
     2101 taatcgaggc gatcgacccc gagcagcagg atctcgcc
//
LOCUS       DASWNJ010001191         2477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_91419, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001191 DASWNJ010000000
VERSION     DASWNJ010001191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91419"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VGB85_20055"
     CDS             complement(<1..447)
                     /locus_tag="VGB85_20055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136393.1"
                     /translation="MRAALVLLLTTPALGACFSDPADPTETTGGGGTSSSGAVSFTTT
                     QDDTPTTGDATASATGDATGDALTTTTNTETTATATGDTGDDTTTGAELPEDEALLRK
                     AIAGEVDPQDALRTIADRGGFPVETSTGSYLFACLCGPGEWQLAGAH"
     gene            complement(495..>2477)
                     /gene="glgB"
                     /locus_tag="VGB85_20060"
     CDS             complement(495..>2477)
                     /gene="glgB"
                     /locus_tag="VGB85_20060"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEY0136394.1"
                     /translation="GVWQGLVPDAAPGMLYKYRVVGLRGEVMDRADPCAQGTELPPQT
                     ASRIVALAHAWNDAAWLAARAQRQGPGAPISVYEVHLGSWLRDAAGDFLTYGEIAPKL
                     VAHVQALGFTHVELMPITEHPFYGSWGYQTSAYFAPTARHGGPEGLMQLIDALHQADI
                     GVILDWVPGHFPADAHGLARFDGTALYEHQDPRRGVHPDWNTLIFNFGRHEVRAFLLS
                     SAMMWIERYHIDGIRVDAVASMLYLDYSRQHGQWLPNERGGRENLEAVAFLRALNAAI
                     HREHPDVITIAEESTSWPQVTGAIEAGGLGFDYKWDMGWMHDTLRYLGRDPIVRQYHH
                     NELTFRMWYAYKERYMLPLSHDEVVHGKYSLIRKMHGSEPARFASLRLLLAYMFTTPG
                     RKLLFMGGEFGQLREWNHDRQLDWELREQPLHAGLERWVARLARLYREVPALHEQDDE
                     PSGYQWLIVDDHLRSLAVYLRFPTRGPVVMIALNFTPVTWVDHAIGVPAEGTWTVVDR
                     SDAHTYSDVEPEAQPASFAASETPAQNLPFSLRVTLPPLTALVLAGPEVAELRAAAER
                     HRVARIAREAEAVRAAEAAAAEAARVAEVAREAQAAAEAEAAEAARVAAEAEAAAAET
                     VAAGIASEKGVRVEEAGRSAADAARRNDPPGSTNER"
BASE COUNT          310 a          901 c          902 g          364 t
ORIGIN      
        1 gtgggcgccc gcgagctgcc actcgccggg gccgcacagg caggcgaaca ggtagctacc
       61 ggtcgaggtc tcgaccggga atcctcctcg atcggcgatc gtgcgcagcg cgtcctgcgg
      121 gtcgacctcg ccggcgatcg ccttgcgcag cagcgcctcg tcctccggga gctcggcgcc
      181 ggtcgtcgtg tcgtcgccgg tgtcgccggt cgcggtcgcc gtcgtctccg tgttcgtcgt
      241 cgtcgtgagc gcgtccccgg tcgcgtcccc ggtcgcgctg gcggtcgcgt cgccggtcgt
      301 cggcgtgtcg tcctgcgtgg tggtgaacga caccgccccg ctgctcgacg tgcctccgcc
      361 gcccgtcgtc tcggtgggat cggcgggatc ggagaagcag gcgccgaggg ccggtgtggt
      421 caggagcagg acgagggcag cacgcacggc cggacttcta gcaggtccgt cgcggttcgg
      481 aaacgcccgc cgcctcagcg ctcgttggtc gagcccggcg ggtcgttgcg ccgcgccgcg
      541 tcggctgccg aacgcccggc ctcctcgacg cgcacgccct tctcggacgc gatgcccgcg
      601 gcgacggtct cggccgctgc cgcctccgct tccgcggcga cgcgcgccgc ctcggcggcc
      661 tccgcctccg cggcggcctg cgcctcgcgg gcgacctcgg cgacgcgcgc cgcctcggcc
      721 gcggccgcct ccgccgcgcg cacggcctcg gcctcgcgcg cgatccgtgc gacgcgatgc
      781 cgctcggcgg ccgcgcgcag ctccgcgacc tcgggccccg cgagcacgag tgcggtcagc
      841 ggcggcagcg tcacgcgcag cgagaagggc aggttctgcg ccggcgtctc gctggccgcg
      901 aagctcgcgg gctgcgcctc gggctcgacg tcgctgtacg tgtgggcgtc gctgcggtcg
      961 acgaccgtcc acgtgccctc ggccggcacg ccgatcgcgt ggtcgaccca ggtcaccggc
     1021 gtgaagttga gggcgatcat caccacgggg ccgcgggtcg ggaagcgcag gtacaccgcg
     1081 aggctgcgca ggtggtcgtc gacgatcagc cactggtagc cggacggctc gtcgtcctgc
     1141 tcgtgcagcg ccgggacctc gcggtagagc cgggcgaggc gggcgaccca gcgctcgagg
     1201 ccggcgtgca gcggctgctc gcgcagctcc cagtcgagct ggcggtcatg gttccattca
     1261 cgcagctggc cgaactcgcc gcccatgaac agcagcttgc ggcccggcgt cgtgaacatg
     1321 tacgcgagca gcaggcgcag gctggcgaac cgggccggct cgctgccgtg catcttgcgg
     1381 atcagcgagt acttgccgtg cacgacctcg tcgtgcgaca gcggcagcat gtagcgctcc
     1441 ttgtacgcgt accacatgcg aaaggtcagc tcgttgtggt ggtactggcg cacgatcggg
     1501 tcgcgcccga ggtagcgcag ggtgtcgtgc atccagccca tgtcccactt gtaatcgaag
     1561 ccgaggccgc cggcctcgat cgcgccggtc acctgcggcc atgacgtcga ctcctcggcg
     1621 atcgtgatca cgtccgggtg ctcgcggtgg atcgcggcgt tgagggcccg caggaacgcg
     1681 accgcctcga ggttctcccg cccgccgcgc tcgttgggca gccactggcc gtgctggcgc
     1741 gagtaatcga ggtacagcat cgaggcgacc gcgtcgaccc ggatcccgtc gatgtgatag
     1801 cgctcgatcc acatcatcgc cgagctgagg aggaacgcgc ggacctcgtg gcgtccgaag
     1861 ttgaagatca gcgtgttcca gtcgggatgc acgccgcgcc gcggatcctg atgctcgtac
     1921 agcgcggtgc cgtcgaagcg cgccaggccg tgggcgtcgg ccgggaagtg tccgggcacc
     1981 cagtcgagga tcacgccgat gtcggcctga tgcagcgcgt cgatcagctg catcaggccc
     2041 tcgggcccgc cgtgacgggc cgtcggcgcg aagtaggcgc tggtctgata gccccacgac
     2101 ccgtaaaacg ggtgctcggt gatcggcatc agctcgacgt gcgtgaagcc cagcgcctgc
     2161 acgtgcgcga ccagcttcgg cgcgatctcg ccgtaggtca ggaagtcgcc ggccgcgtcg
     2221 cgcagccacg acccgaggtg cacctcgtac accgagatcg gcgcgccggg accctgccgc
     2281 tgcgcccgcg cggccagcca tgcggcgtcg ttccaggcgt gcgcgagcgc gacgatccgc
     2341 gaggcggtct gcggcggcag ctcggtcccc tgcgcgcacg gatcggcgcg gtccatcacc
     2401 tcgccgcgca ggccgacgac gcgatacttg tagagcatgc ccggcgcggc gtcgggcacc
     2461 agcccctgcc acacccc
//
LOCUS       DASWNJ010001192         2064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_373267, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001192 DASWNJ010000000
VERSION     DASWNJ010001192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373267"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="VGB85_20065"
     CDS             complement(<1..1314)
                     /locus_tag="VGB85_20065"
                     /inference="COORDINATES: protein motif:HMM:NF040941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0136395.1"
                     /translation="MNGCGDDGGATSGFTTVPQTSESTMMTIGATTTGTPTSTTDPST
                     GAPTTGESTTAITGDTTGEPSTGGPVCGDGILAGDEQCDEGPANADDGACTSQCMTAV
                     CGDGLVLASVEACDDGNVAAGDGCAADCTLESCGNGKVEPPEACDDGNTEDADACRNT
                     CVAAACGDGVLYTDMEGCDDGNLADDDECLATCVAATCGDGFVWSGMEVCDDGDLDAI
                     ECTADCVLPTCMDAAQNGVETDVDCGGAACPKCAVMKKCAAASDCASGVCTDAVCTPP
                     PSCKAILAADPAAPSGKYNLDLDGVGPLKPFNGHCDMTTDGGGWTVFYAVSGADAEQA
                     MVSDAELLLNDPLVFQAFNLNRARKIALSQISAATLFVRTGNVWLRADKPAFDADLNV
                     PNTTSKGPVMLTSSDGVTAAAFMGYANFNYNGGGDFGLSMSPDAAT"
     gene            1475..>2064
                     /locus_tag="VGB85_20070"
     CDS             1475..>2064
                     /locus_tag="VGB85_20070"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0136396.1"
                     /translation="MGELRLSDINMNLLLALEALLVEANVTRAARRVHVTQSAMSHTL
                     AQLRELLGDPLLIRGRGGMVLTPRAQALAAPLGLGLRQLQRALGEEVGFDPASTTRCF
                     TLAMGDFVSVLLMPALLQRLRAAAPAITLEVAAIDRRRNAELLESGEHDLVVGVRLEP
                     APGLIVDPLISQRFVCVVREGHPEIDGSISLDQYER"
BASE COUNT          249 a          758 c          732 g          325 t
ORIGIN      
        1 ggtcgccgcg tcgggtgaca tcgacaggcc gaagtctccg ccgccgttgt agttgaagtt
       61 cgcgtagccc atgaacgccg ccgccgtcac gccgtcgctg ctggtcagca tcaccgggcc
      121 cttggaggtc gtgttgggca cgttgaggtc ggcgtcgaag gccggcttgt cggcgcgcag
      181 ccagacgttg ccggtccgca cgaacaaggt cgcggccgag atctggctca gcgcgatctt
      241 gcgggcgcgg ttgaggttga acgcctggaa cacgagcggg tcgttgagca gcagctcggc
      301 gtcgctgacc atcgcctgct ccgcgtcggc gccgctgacc gcgtaaaaca cggtccagcc
      361 gccgccgtcg gtcgtcatgt cgcagtggcc gttgaacggc ttgagcgggc cgacgccgtc
      421 gaggtcgagg ttgtacttgc cgctcggggc cgccggatcg gcggcgagga tcgccttgca
      481 gctcggcggc ggcgtgcaca cggcgtcggt gcacacgccc gaggcgcagt cgctcgcggc
      541 cgcgcacttc ttcatgaccg cacacttcgg gcaggccgcg cccccacagt cgacgtcggt
      601 ctcgacgccg ttttgcgcgg cgtccatgca ggtcggcagc acgcagtccg cggtgcactc
      661 gatcgcgtcg agatcgccgt cgtcgcacac ctccatgccg gaccacacga agccgtcgcc
      721 gcaggtcgcc gcgacgcagg tcgccagaca ctcgtcgtcg tccgcgaggt tgccgtcgtc
      781 gcacccctcc atgtccgtgt acagcacgcc gtcgccgcag gccgccgcga cgcaggtgtt
      841 gcggcacgcg tcggcgtcct cggtgttgcc gtcgtcgcac gcctcgggcg gctcgacctt
      901 gccgttgccg cagctctcga gcgtgcagtc ggcggcgcag ccgtccccgg ccgcgacgtt
      961 gccgtcgtcg caggcctcga cgctcgcaag cacgagcccg tccccgcaga ccgcggtcat
     1021 gcactggctc gtgcacgcgc cgtcgtcggc gttggccggg ccctcgtcgc actgctcgtc
     1081 gccggcgagg atgccgtcgc cgcacacggg cccgcccgtc gacggctcgc cggtcgtgtc
     1141 gccggtgatc gcggtggtcg actcgccggt cgtcggcgcg ccggtcgagg ggtccgtggt
     1201 cgacgtcggc gtgccggtcg tcgtcgcgcc gatcgtcatc atcgtcgact cgctcgtctg
     1261 cggcaccgtc gtgaagccgc tcgtggctcc gccgtcatcg ccacacccgt tcatcaccac
     1321 catcgtcgcg acagcgacgc cgatcccacg catacccatg acgcgcaggg tagcggccgg
     1381 gcgccgcgct gcccagcgtc cgcgacgcaa gtccgctcac gagcgcgcgc aggcgcgtgt
     1441 tatggtccgg cgcggggcgg cgggggacgg gactgtgggc gagcttcgac tctcggacat
     1501 caacatgaac ctgttgctgg cgctcgaggc cctgctcgtc gaggccaacg tgacgcgggc
     1561 ggcgcggcgc gtgcacgtga cgcagtcggc gatgagccac accctcgcgc agctgcgcga
     1621 gctgctcggc gacccgctgc tgatccgcgg ccgcggcggc atggtcctga ccccgcgcgc
     1681 gcaggccctc gcggccccgc tggggctcgg gctgcgccag ctccagcgcg cgctcggtga
     1741 ggaggtcggc ttcgatccgg cgagcaccac gcgctgcttc acgctggcga tgggtgactt
     1801 cgtatcggtg ctgctgatgc cggcgctgct ccagcgcctg cgcgcggcgg ccccggcgat
     1861 cacgctcgag gtcgcggcga tcgaccgccg gcgcaacgcc gagctgctcg agtcgggcga
     1921 gcacgacctc gtcgtcggcg tgcgcctcga gcccgcgccc ggcctgatcg tcgacccgct
     1981 gatctcgcag cgcttcgtgt gcgtggtccg cgagggccac ccggagatcg acggcagcat
     2041 ctcgctcgat caatacgagc gctg
//
LOCUS       DASWNJ010001193         3528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_480425, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001193 DASWNJ010000000
VERSION     DASWNJ010001193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480425"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VGB85_20075"
     CDS             <1..386
                     /locus_tag="VGB85_20075"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEY0136397.1"
                     /translation="QPFGNSLVVMTLTGAQIKTMLEQQFSPGYDNVLHVSKGFTYTYK
                     QSAPIGMKVDAASIKLNGVLIAPGQKYRVTVISYLAAGGDSFSVLVDGTERLGGVTDM
                     EALEDYLADNSPVSPPPLDRITVVP"
     gene            528..1736
                     /locus_tag="VGB85_20080"
     CDS             528..1736
                     /locus_tag="VGB85_20080"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0136398.1"
                     /translation="MTETFAFNPFVPGFDEDPAPFYRELRTRAPVYFWPMAEAYLVSR
                     YDDILAMMKDPRLSRSQRDGKRYQPLPDVPEYVEYRAATENGLFQVGREDHLRLRRLV
                     NPGFTPRAIELLRADIHAITVAALARLPDEEVVDLAAMIDDVPLRVIGRLLAIPAEME
                     AGFLTFARIRLLLISPGLGEDERDELVRSLAPGYGDIRRLISDRRRAPGADLLSNLIH
                     LEEQGTRLTEPELLGLVESIIIGGADTTVHTLRFMLLNLLRNPQQAARVSQDPTLARA
                     AMDETLRFDHFNKFGTPAYALEDLTIGGVEIAKGDTVLPMRAAGSRDPEVFARPDEFD
                     IGRADLDAAHNFGLGPHTCLGMHLARLQAEIVVPTILRRFPRMTLAGPPEFTPHLFFR
                     VMSRLPVRTG"
     gene            complement(1829..2236)
                     /locus_tag="VGB85_20085"
     CDS             complement(1829..2236)
                     /locus_tag="VGB85_20085"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0136399.1"
                     /translation="MRPQPLIAVNDVEVSSRWYQRLLGCRSDHGGGEYERLLDGAGRL
                     VLQLHAWEVEHGHGKIGDASKPRGNGVLLWFEVDDFDAAVTRSGELRAEVVLGVHRNP
                     PAGEPGGPAHREMWIRDLDGYTVVLVSPDGEAA"
     gene            complement(2388..>3528)
                     /locus_tag="VGB85_20090"
     CDS             complement(2388..>3528)
                     /locus_tag="VGB85_20090"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0136400.1"
                     /translation="VPIVNLISLIRRSSTMTLRDALRVDDTLGQAMMAYDPEATYAKY
                     DHMSAAEFLDRLGFPPRARQMLFDVFAHSFFNPEASLSAAELMMMFHFYFIGNPEGIV
                     FDVLDGPFSTRLWQPMQRWLEQRGVQILLGEGVERLQREDTCRWSVTTSTRTLCAEAV
                     VLAPHVDGLRKIIAASPTVGDATWRAAIAGQTVTNAFAVWRLWLDTPCNPGRSPFVGT
                     TGLGLLDNISLYHLFEDESRDWAARTGGSVVELHAYALPDGLAEPQIKADMLATLHLL
                     YPETRGARIVEQRYLQHQDCPAFRPGTAATRPRVATPEPDLVLAGDLVHTDFPSALME
                     RAASTGMLAANLLLQRRGVAEEPLHSVVPRGMFARLVHRGFKRSA"
BASE COUNT          553 a         1203 c         1234 g          538 t
ORIGIN      
        1 tccagccctt cggcaacagc ctcgtggtga tgacgctgac cggcgcgcag atcaagacga
       61 tgctcgagca gcagttctcg cccggctacg acaacgtgct gcacgtctcg aagggcttca
      121 cgtacacgta caagcagtcc gcgccgatcg ggatgaaggt cgacgccgcc agcatcaagc
      181 tcaacggcgt gctgatcgcg cccgggcaga agtatcgcgt cacggtgatc agctacctgg
      241 ccgccggcgg cgacagcttc tccgtgctgg tcgacggcac cgagcggctc ggcggtgtca
      301 ccgacatgga ggcgctcgag gactacctgg ccgacaactc gccggtctcc ccgccgccgc
      361 tcgaccgcat caccgtggtg ccctgacgaa caggcgcagc gcgttgatcg acgagaagag
      421 ccacgacctc cgattttacc gggggccgtg ccggcgtggc aaggcggcgt gtacacgcgc
      481 gacgccggac gtgcgacact ttccatgagg cgcatcagga gcacggcatg acggagactt
      541 tcgcgttcaa ccccttcgtg cccggcttcg acgaggatcc cgcgccgttt taccgcgagc
      601 ttcgcacccg cgccccggtg tacttctggc cgatggcgga ggcgtacctc gtcagtcgtt
      661 atgacgacat cctggcgatg atgaaggacc cgcgattatc gcggtcgcag cgggacggca
      721 agcgttacca gccgctgccg gacgtgcccg aatatgtcga gtaccgtgcg gcgaccgaga
      781 acgggctgtt ccaggtcggc cgcgaggatc acctgcggct gcgccggctg gtgaacccgg
      841 gattcacgcc gcgggcgatc gagctgctgc gcgcggacat tcacgcgatc accgtcgcgg
      901 cgctggcccg actcccggac gaggaggtcg tcgacctcgc ggcgatgatc gacgacgtcc
      961 cgctgcgcgt gatcgggcgc ctgctggcga tccccgcgga gatggaggcc ggctttctca
     1021 ccttcgcgcg gatccgcctg ctgctgatca gccccgggct cggcgaggac gagcgcgacg
     1081 agctggtccg cagcctcgcg ccgggatatg gcgatattcg ccggctgatc tccgatcgca
     1141 gacgagcgcc cggcgccgac ctgctgtcga acctcattca tctcgaggag cagggcacgc
     1201 gcctgaccga gcccgagctg ctcgggctgg tcgagtcgat catcatcggt ggcgccgaca
     1261 cgacggtgca cacgctgcgc ttcatgctgc tcaacctcct gcgcaacccg cagcaggccg
     1321 cgcgcgtctc ccaggacccg acgctcgctc gcgccgcgat ggacgagacc ctgcgcttcg
     1381 accattttaa taagttcggc acacccgcct atgcgctcga agatctgacg atcgggggcg
     1441 tcgagatagc gaagggggac accgtgctgc cgatgcgggc cgccggttcg cgggatcccg
     1501 aagtgttcgc gcgacccgac gagttcgata tcggccgcgc cgacctcgac gctgcccata
     1561 acttcgggct cggcccgcac acctgcctcg ggatgcacct cgcccgcctg caggccgaga
     1621 tcgtggtgcc gacgatcctg cgccggttcc cacgcatgac cctcgcgggg ccccccgagt
     1681 tcacgccgca cctgttcttt cgcgtcatga gccgcctgcc cgtacggaca ggttgagaaa
     1741 gacggtcgcg cgccgacagc ccggacgcga cagagcgcac catggcgggc gcgtctatgt
     1801 gtggcaggcg aagtcagcag gcgccggatc aggccgcctc gccgtcgggg ctgaccagta
     1861 cgaccgtgta gccgtcgagg tcgcggatcc acatctcgcg gtgggccgga ccgcccggct
     1921 cgcccgcggg cgggttgcga tgcacaccga gcacgacctc ggcccgcagc tcgccgctgc
     1981 gagtcaccgc cgcgtcgaag tcgtcgacct cgaaccacag cagtacgccg ttcccgcgcg
     2041 gcttgctcgc atccccgatc ttgccgtgac cgtgctcgac ctcccaggcg tgcaactgca
     2101 gcaccaggcg gcccgcgccg tcgagcagcc gctcgtactc acctccgccg tggtcgctgc
     2161 ggcacccgag cagacgctga taccagcgac tgctcacctc gacatcgttc acggcgatca
     2221 atggttgggg tcgcatcgag ctccctcatc tcgcaagtca ccggcatcgt caatgctgcc
     2281 gggcccggag agtcgggccg tcccgcggtc gattcggtcc gcgaccctcc ccgggctcag
     2341 ggtcccgcga cgggctcggg cttcccccat gtttgacccg gctggcctca ggcgctgcgc
     2401 ttgaagccgc ggtgcacgag ccgggcgaac ataccgcgcg ggaccaccga gtgcagcggc
     2461 tcctcggcga cgccgcggcg ctggagcagc aagttggcgg caagcatgcc ggtcgaggcg
     2521 gcgcgctcca tcagggcgct cgggaagtcg gtgtgcacga ggtcgccggc gagcacgagg
     2581 tcgggctcgg gggtggcgac gcgcgggcgg gtggcggcgg tgcccggacg aaacgccggg
     2641 cagtcctgat gctggagata tcgctgctcg acgatccgcg cgccgcgggt ctcggggtac
     2701 agcaggtgca gggtggcgag catgtcggcc ttgatctgcg gctcggcgag gccgtccggc
     2761 agcgcgtagg cgtgcagctc gacgacgctg ccgccggtgc gcgcggccca gtcgcggctc
     2821 tcgtcctcga acaggtgata tagcgaaatg ttgtcgagca ggccgaggcc ggtggtgccg
     2881 acaaaggggg agcgcccggg gttgcacggc gtgtcgagcc acaggcgcca caccgcgaat
     2941 gcattggtga cggtctggcc ggcgatcgcg gcgcgccagg tcgcgtcgcc gacggtcggg
     3001 gaggcggcga tgatcttgcg caggccgtcg acgtgcgggg cgagcacgac ggcctcggcg
     3061 cagagcgtgc gtgtgctcgt cgtgacggac cagcggcagg tatcttcgcg ttgcaggcgc
     3121 tcgacgccct cgccgagcag gatctggacc ccgcgctgct cgagccagcg ctgcatcggc
     3181 tgccacaggc gggtcgagaa cgggccgtcg agcacgtcga agacgatgcc ctcggggttc
     3241 ccgatgaagt agaagtgaaa catcatcatc agctcggcgg ccgacaggct ggcctccggg
     3301 ttgaagaagg agtgcgcgaa cacgtcgaag agcatctgcc gggcgcgcgg cgggaagccg
     3361 aggcggtcga ggaactcggc ggcgctcata tgatcgtact tggcgtaggt cgcctcgggg
     3421 tcgtaggcca tcatcgcctg gccgagcgtg tcgtcgacgc gcagcgcatc gcgcagggtc
     3481 atcgtcgacg agcgacggat caggctgatg aggttgacga tcgggacg
//
LOCUS       DASWNJ010001194         4506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_22394, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001194 DASWNJ010000000
VERSION     DASWNJ010001194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22394"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="VGB85_20095"
     CDS             <1..1062
                     /locus_tag="VGB85_20095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0136401.1"
                     /translation="AEGPTGAVAGAELDTSEAVPRGPIRLAFRTLRPVSLDDPEHAAL
                     RDPYLARTRPRSALCLPLVMQGQAQGVLYLENSVAAGAFTPARRETLAMLSTQIAISL
                     QHAKLYAHLEQARAAAEAASRTKSTFLANMSHELRTPLNAIIGYADLIEEEIEARGSG
                     SEDLSDIRKIQRAGRHLLDIISDILDLSKIEADKLDIFVQDFALAPVVSEVLETVRPA
                     MQRGGNRLVFNHEAPLGTLRSDPVRLRQVLLNLLSNSAKFTQDGTVTLEIVRAGGRIR
                     FAVEDTGIGMSQAELERIFDAFHQVDASATRQVGGTGLGLTISRHLCQLLGGDIEVSS
                     TPGVGSKFTIDLPADPVHT"
     gene            1118..1708
                     /locus_tag="VGB85_20100"
     CDS             1118..1708
                     /locus_tag="VGB85_20100"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0136402.1"
                     /translation="MKLYHAHHTSAFRCRWMLEEAGLPYELINISLGKGEHKRPEYLQ
                     IHPLGSVPALVDGEHNYIESAGIVMHVADMDPEQRFAPAIGDPRRGHYYQWILFAMTN
                     LYQVVHPAYLRYFFKRETASSEDRDALSKQLGPIDDILNKSPFLLGEQFTGADVIVGG
                     TLLWAESCGLLLGREPQASYLERLKARPAFQRAAAE"
     gene            1723..2160
                     /locus_tag="VGB85_20105"
     CDS             1723..2160
                     /locus_tag="VGB85_20105"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY0136403.1"
                     /translation="MEPRAHVRLVAVMNHDPVEFERVSSRAIWEERVGYCRALRAGNL
                     VFVTGTAPVDDSGSGVHAVGDAYAQARRCLELIEHAIAQFGVDRTRIVRTRMFVTDIS
                     RWEEFGKAHSEFFGEHRPTTSMVEVRRLIDPDMLIEIEADAVA"
     gene            complement(2227..>4506)
                     /locus_tag="VGB85_20110"
     CDS             complement(2227..>4506)
                     /locus_tag="VGB85_20110"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF009520.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEY0136404.1"
                     /translation="VLNQGDIYYPDSLVGTDSHTTMINGLGIVGWGVGGIEAEAGMLG
                     QPVYFLTPDVVGFHLKGALPPGTTATDLVLTVTEVLRKHKVVGKFVEFFGEGAASLSV
                     TDRATLGNMAPEYGATMGFFPVDEKTCAYLRDTGRSEAQVDAVRQYHVAQGMFGMPAD
                     GELDYSARVELDLSTIEPSVAGPKRPQDRIALGQLGSKFNELLHSPKEYARPEAESRR
                     RLQVVTGSGSGIISGGGSQTAIPEEINRVTTPSNTNPLTEIEMMHNRPTPDRVQDSEP
                     APERVVLRHGDVVIAAITSCTNTSNPSVMLAAGLLAKKAVERGLKVGKSVKTSLAPGS
                     RVVTAYLEKTGLQPFLDKLGFQTVGYGCTTCIGNSGPLDPQLEAAIQDNNVVVASVLS
                     GNRNFEARIHQAIRANFLMSPPLVVAFALAGRVDIDLATESLGHDSEGKDVYLRDLWP
                     TSEEIGELLTSATDPATYREIYGDTGALSPMWAEIPTSTGKVYPWDESSTYIQEPPYF
                     EKFSVEPAPTKSIAGARPLAIFGDSVTTDHISPAGSIQPSSPAGKHLIERGVGVDDFN
                     SYGARRGNDRVMVRGTFANVRIRNLMVAGVEGGVTVHQPTGERMSIYDASIRYQAAGI
                     PLIIFAGQEYGTGSSRDWAAKGSRLLGVRAVVATSFERIHRSNLVGMGVLPCQFQSGT
                     DAQSLGLDGSETFDLTGLDTVTPRATLTLKIHRTSGKVDDVPVTLRIDTPIEVEYYLH
                     GGILPYVLRQRVASTGAAS"
BASE COUNT          682 a         1523 c         1586 g          715 t
ORIGIN      
        1 gcggagggcc cgaccggcgc cgtcgccggc gccgagctcg acaccagcga ggccgtccca
       61 cgcggaccga tccgcctcgc gtttcgcacc ctgcgacccg tctccctcga cgaccccgag
      121 cacgccgccc tgcgcgaccc ctacctcgcc cgcacgcgcc cgcgctcggc cctgtgcctg
      181 ccgctcgtca tgcagggcca ggcgcagggc gtgctgtatc tcgagaacag cgtcgcggcc
      241 ggggccttca cgccggcccg gcgcgagacc ctggcgatgc tgagcacgca gatcgcgatc
      301 tcgctgcagc acgccaagct atacgcccac ctcgagcagg cgcgcgcggc cgccgaggcc
      361 gccagccgga ccaagagcac cttcctcgcc aacatgagcc acgagctgcg gaccccgctc
      421 aacgcgatca tcggttacgc cgacctgatc gaggaggaga tcgaggcccg cggcagcggc
      481 agcgaggacc tgtccgacat ccgcaagatc cagcgcgcgg ggcggcacct gctcgacatc
      541 atcagcgaca tcctcgacct ctcgaagatc gaggccgaca agctcgacat cttcgtccag
      601 gacttcgccc tcgcccccgt ggtctccgag gtgctcgaga ccgtgcgccc ggcgatgcag
      661 cgcggcggca accgcctggt cttcaaccac gaggccccgc tcgggaccct gcgcagcgat
      721 cccgtgcgcc tgcgccaggt cctgctcaac ctgctcagta actcggcgaa gttcacgcaa
      781 gacggcaccg tcaccctcga gatcgtgcgc gccggcgggc ggatccgctt cgcggtcgag
      841 gacaccggca tcggcatgag ccaggccgag ctcgagcgca tcttcgacgc gttccatcag
      901 gtcgacgcct ccgcgacccg ccaggtcggc ggcaccggcc tcggcctgac gatcagccga
      961 cacctgtgtc agctgctcgg cggcgacatc gaggtgtcca gcaccccggg cgtcggctcg
     1021 aagttcacga tcgacctgcc cgcggacccg gtccacacct gaagtccacg cacgaaccgt
     1081 ggtggcgtcc gccctcgctt tgcgcgatcg tcgggccatg aagctgtacc acgcccacca
     1141 cacctcggcc tttcgctgcc gatggatgct cgaggaggcc ggcctcccct acgagctgat
     1201 caacatcagc ctcggcaagg gcgaacacaa gcggcccgag tacctgcaaa ttcacccgct
     1261 cggctcggtg cccgcgctcg tcgacggcga gcacaactac atcgaatccg ccggcatcgt
     1321 catgcacgtc gccgacatgg accccgagca gcgcttcgcc cccgcgatcg gcgacccccg
     1381 gcgcggccac tattatcagt ggatcctgtt cgcgatgacc aatctctatc aagtggtcca
     1441 cccggcctac ctgcgctact tttttaagag ggagaccgcc tccagcgagg atcgcgacgc
     1501 tctttcaaag cagctcggcc cgatcgacga cattcttaat aaatccccgt tcctgctcgg
     1561 ggagcagttc actggggccg acgtcatcgt cggcggcacg ctcttgtggg ccgagtcctg
     1621 cggcctgctg ctcggccgcg agccgcaagc ctcgtacctc gagcgcctca aggcccgccc
     1681 cgccttccag cgcgccgccg ccgagtaggc gcgagcccga gcgtggagcc acgcgcccat
     1741 gttaggctcg tggccgtcat gaatcacgac cccgtcgagt tcgagcgcgt gtcctctcgt
     1801 gcgatctggg aggagcgcgt cggctactgc agggcgctgc gcgccggcaa tctggtcttc
     1861 gtgacaggta ccgcgccggt cgacgacagc ggctccggcg tgcacgcggt cggcgacgcg
     1921 tacgcacagg cccggcgctg cctcgaactg atcgagcacg cgatcgcaca attcggggtc
     1981 gaccgcacgc ggatcgttcg cacgcggatg ttcgtcaccg atatctcgcg ctgggaagag
     2041 tttggtaagg cccactcgga gttctttgga gaacaccgac cgacgacctc gatggtcgag
     2101 gtccgccggc tgattgatcc ggatatgctg atcgagatcg aggcggacgc ggtggcctga
     2161 tccgcggcac gaacgaacgc ggcccgccgt gagggcgagc cgcgtctttc agggcgcccg
     2221 agatggtcag gacgcggcgc cggtgctggc gacgcgctgg cggagcacgt aggggaggat
     2281 gccgccgtgg aggtagtact cgacctcgat cggggtatcg atgcgcaggg tgacgggcac
     2341 gtcgtcgacc ttgccgctgg tgcgatggat cttcagggtg agggtcgcgc gcggggtgac
     2401 ggtgtcgagg ccggtgaggt cgaaggtctc gctgccgtcg aggccgaggc tctgggcgtc
     2461 ggtgccggac tggaactggc agggcagcac gcccatgccg acgaggttgc tgcggtggat
     2521 gcgttcgaag gaggtcgcga ccacggcgcg gacgccgagc aggcgcgagc ccttggcggc
     2581 ccagtcgcgc gaggagccgg tgccgtactc ctggccggca aagatgatca gcgggatgcc
     2641 ggcggcctgg tagcggatcg acgcgtcata gatgctcatg cgctcgccgg tcggctggtg
     2701 gaccgtgacg ccgccctcga cgcccgcgac catgaggttg cggatccgga catttgcaaa
     2761 agtgccgcgg accatgacgc ggtcgttgcc gcgacgggca ccgtacgagt tgaagtcgtc
     2821 gaccccgacg ccgcgctcga tcaggtgctt gccggcgggg gacgacggct ggatgctgcc
     2881 ggcggggctg atgtggtcgg tggtgacgct gtcgccgaag atcgccagcg ggcgggcgcc
     2941 ggcgatcgac ttggtcgggg ccggctcgac gctgaacttc tcgaagtagg gcggctcctg
     3001 gatgtacgtc gaggactcgt cccacgggta gaccttgccg gtgctcgtgg ggatctcggc
     3061 ccacatcggg ctcagcgcgc cggtgtcgcc gtagatctca cgatacgtcg cggggtcggt
     3121 cgcgctggtc agcagctcgc cgatctcctc gctggtcggc cacaggtcgc gcaggtacac
     3181 gtccttgccc tcgctgtcgt ggccgagcga ctcggtggcg aggtcgatgt cgacgcggcc
     3241 ggcgagcgcg aaggcgacga cgagcggcgg gctcatcagg aagttggcgc ggatcgcctg
     3301 atggatgcgg gcctcgaagt tgcggttgcc cgagagcacg ctggcgacga cgacgttgtt
     3361 gtcctggatc gcagcctcga gctgcgggtc gagcgggccg gagttgccga tgcaggtggt
     3421 gcaaccataa ccgacggtct ggaacccgag cttgtcgagg aagggctgca ggccggtctt
     3481 ctcgaggtaa gcggtgacga ccctcgagcc cggcgcgagc gaggtcttga ccgacttgcc
     3541 gaccttgagg ccgcgctcga cggccttctt ggcgagcagg ccggcggcga gcatgacgct
     3601 ggggttcgag gtgttggtgc agctggtgat cgcggcgatg acgacgtcgc cgtggcgcag
     3661 cacgacgcgc tcgggcgcgg gctcgctgtc ctgcacgcgg tccggcgtcg gccggttgtg
     3721 catcatctcg atctcggtca gcgggttggt gttgctcggg gtggtcaccc ggttgatctc
     3781 ctcggggatc gccgtctgcg agccgccgcc gctgatgatg ccgctcccgg agccggtgac
     3841 gacctgcagg cgacgccggg actcggcctc gggacgcgcg tactccttgg ggctgtgcag
     3901 cagctcgttg aacttgctgc cgagctggcc gagcgcgatg cggtcctgcg ggcgcttggg
     3961 gccggcgacg ctcggctcga tcgtcgagag gtcgagctcg acgcgggcgc tgtagtcgag
     4021 ctcgccgtcg gccggcatgc cgaacatgcc ctgcgcgacg tgatactggc gcacggcgtc
     4081 gacctgggcc tcgctgcggc cggtgtcgcg caggtaggcg caggtcttct cgtcgaccgg
     4141 gaagaagccc atcgtcgccc catactcggg ggccatgttg cccagagtgg cccggtcggt
     4201 gacggacagc gacgcggcgc cctcgccgaa gaactcgacg aacttgccga cgaccttgtg
     4261 cttgcgcagg acctcggtga cggtgagcac gaggtcggtg gcggtggtgc cgggcgggag
     4321 ggcgcccttg aggtggaagc cgacgacgtc cggggtgagg aagtagaccg gctggccgag
     4381 cataccggcc tcggcctcga tgccgccgac gccccagccg acgatgccga ggccgttgat
     4441 catggtggta tgcgagtcgg tgccgaccag cgagtccggg taatagatat caccctggtt
     4501 gagcac
//
LOCUS       DASWNJ010001195         8961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_404254, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001195 DASWNJ010000000
VERSION     DASWNJ010001195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 8961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8961
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404254"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VGB85_20115"
     CDS             <1..247
                     /locus_tag="VGB85_20115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HEY0136405.1"
                     /translation="PTNPNGSTESDGPTGGETPTGGETPTGSGTDTGLTDGTGSGGAT
                     ETGGQDEAGGCSCRSDAPNGLAGGLLGLGLLGLRRRR"
     gene            complement(248..1255)
                     /locus_tag="VGB85_20120"
     CDS             complement(248..1255)
                     /locus_tag="VGB85_20120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136406.1"
                     /translation="MSATSEDELHFVQQLIARERLSLDLCARGRTSFLSHVGAPPRLV
                     PGPWIGHRDLIYALALANLAPAQRTALARAVLVEHDREFGVGRLQLRTLPSGLPLGGS
                     SLRLQPRGPGSGPSCLYTWALGASARAAPCEWLLLRAEPCWSLPDPPRPLTPEAIETL
                     HALSASVVILVATAPDARMVADRCLRNVPFTAHPRFLPHLEGDDSKVTRPTGAEPARV
                     RLWPHDAVNAPGFWPTHRGKPAETAGAVLLIDAPEPVRQQVQAWHAQHPEARRIEVAR
                     ASCPGRADRAAIAAFWEASERPQILLRGDPEWAGDARRFLQDLGARVEAQAQGTQLAL
                     L"
     gene            complement(1252..1770)
                     /gene="tadA"
                     /locus_tag="VGB85_20125"
     CDS             complement(1252..1770)
                     /gene="tadA"
                     /locus_tag="VGB85_20125"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEY0136407.1"
                     /translation="MLATTDLDLVYMREALRLAGTAAARGDVPVGAIVVMNGAITGVG
                     FNTREAAADPTGHAEIVALREACRREGRWRVDGATLYVTLEPCPMCAGALVNARVARL
                     VYGAADPKAGAARSLFSLCDDPRLNHRMEVVPGVLAGPCGEVLSKFFRGLRRSGRDLS
                     SGTASAIPGVSS"
     gene            1925..2053
                     /locus_tag="VGB85_20130"
     CDS             1925..2053
                     /locus_tag="VGB85_20130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136408.1"
                     /translation="MTLAAGCGGEVVTQQIQQRRRARREIGPAQAQAGQRGQQQRG"
     gene            complement(2060..3643)
                     /locus_tag="VGB85_20135"
     CDS             complement(2060..3643)
                     /locus_tag="VGB85_20135"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY0136409.1"
                     /translation="MKQFLFDLLTRFRGVILVALLPVGYIFDAALRLRNFIYERLIAD
                     PSQHDLRVRRVQEQVRRWYAQPAEQRKPMCTDRRTWQNLSTRFAPKHAWHKIQMAGLC
                     NVLGVDLGRRTVRVEPFVTVGQATRYLLPRGHMLSVTLEIEDATIGGLAMAVGMTTHS
                     HKVGLLFETIVAYEVVIADGTLLKVTRESHPDLFHALPWSHGTLGLLVALELQIMPVK
                     SHVRLRYIPVHTQQQYCDQLHAMAVASDAPDFLEATVFAKDHAVIMAGDFADAPADRS
                     EVNPLGRWYKPWFFKHVETFLWREQGGEEYIPLEHYLMRHNRSIFWVVQHMIPFGNHP
                     AFRWLLGWLMPPRIAFIKFSTTPAVREMTFTKQVFQDIVLPMSAMSRAIDASHRLFEM
                     YPVLLYPSRIYDHGALQGQLRAPRDQDRVPGTDYGMFFDLGVYGVPKPILEGKPFKTV
                     HAMRKMEEFTQEVGGYPFLYADTFMTREEFEKMFSLDLYRQVRAKYGADAAFPDLYDK
                     IKPEVDVFAVLEREAQQPD"
     gene            complement(3640..4788)
                     /locus_tag="VGB85_20140"
     CDS             complement(3640..4788)
                     /locus_tag="VGB85_20140"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0136410.1"
                     /translation="MANTVKLGQLPAESQSLVGRWRLVLGKDAEASGLGLAPGGGAGG
                     GENQAGAEAALSFVYGESRGGGAEESQLTVPKWIDQVNELFPKQAREVLEREMIQKKG
                     IAQILDQPQLLDRIEPNVELVKTLLTHKDLLTPKTRILARKVIDKVVQDLKKKLEIRV
                     EQSITGALRKDRHSPRRVFRNLDLKTTLRRNLRNWNHERRKLFVDRIFFYAAEKNKRP
                     WHVIVCVDQSGSMLESAIFSAVMASIFAELPGVKTSLVLFDTQVVDLTDRVGAPVDVL
                     LSVQLGGGTDITKGLEYCSGLVREPARTIIVLITDFYEGRPEEDLIRVVARLAGADVR
                     MIGLGALGYDAQPQYNRATAKKLRKVGMDILSCTPEKLADCMGEIIRG"
     gene            complement(4807..7272)
                     /locus_tag="VGB85_20145"
     CDS             complement(4807..7272)
                     /locus_tag="VGB85_20145"
                     /inference="COORDINATES: protein motif:HMM:NF040143.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HEY0136411.1"
                     /translation="MQDPGANVLEAVDATPGLDASAVEATAMQVLGEPLYWFPVRHHS
                     PAAARYLAEALRARRPKIIFIEGPAEATELVQHIQDSKTKPPIAIYSSYRDDDNVLGL
                     AGIASSAPDVPAKWGVWYPMLPYSPEYVAIKVAKEIGAQVVFIDLPHHGLIEPHREDA
                     PAEEEETEETPSAEPPTWENLAVESSFFKVLAETAGYRSWSECWDALFEVGGRHGDAE
                     AFRRDMAFFCAALRATTSDASMQHDGTFAREAHMWRAISTNLGEQRLRPEDAVVVCGG
                     FHLFMRREVLPAKPLPAGTVYATVAPYSYYRTSEHSGYGAGNRAPKYYEMLYQHMQGP
                     APEEAPIRAMVDHVVAVLSRGRRAGELLSSADAISITQHARMLASLRGHQAPTLDDVR
                     DGVLSCCCKGRPEEEGKYLLTAMSEVEIGTAIGRVTPALGRLPLVFDFYRSIDELDLG
                     ETMGKEKKHKLTIDLRQEAGERRSVFFHRLAELGVSFATIVDTGETNTLFREVWRVEW
                     SPKVETQLIENNLYGDTMEAAAAAKIDEELAKEQDDAASVCARLLRATNMDLPGLVLR
                     IEATAGHAIDADTRLVSLAQALTHLVVLRRQAVRKKIREDIVIDLQGRCFARACFAIP
                     EVANVPVEDHEDVLSALKSIAEALLGDSDAGFDRDLFVESVRSAYAQSNSPYLRGAMC
                     GLLTETRAQPAEVLSQEILKFAQSRPEVMLTCGEFLDGVMATSRTAIMLGAQSLVEAF
                     DVLLRAASWDQFLVMLPRTRKAFERLHDRSRVAFADRVAQLYGLQEGEGDKLAQLDTS
                     AGAAVWMARIDARVAEVMKEWDF"
     gene            complement(7281..8477)
                     /locus_tag="VGB85_20150"
     CDS             complement(7281..8477)
                     /locus_tag="VGB85_20150"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0136412.1"
                     /translation="MAKAKTTPADVSAPPEPGSAPVAPESELLREPAEVKYRRELEAL
                     RQNEADQAPAAWKLSPRSVLTYIVGGKKPLKATIDGKVVEVTITRKFFGDDSIVERSI
                     VTLASERALLLVGDPGTGKSWLSEHLAAAISGTSLLTIQGTAGTTEEQIKYSWNIARI
                     IAEGPKPDNMIQSPCMLAMRAGKIFRFEEITRCVGDVQDALVSICSDKTIAVPELPDN
                     PMVFAKPGFNIIATANARDQGVNELSAALKRRFNYIHIPVVSDQKTEVEIVRTRSAEL
                     LERYKIPGRIEEPVLRLLATVFREMRQGTTGEGLSLQKPSTTLSTAEAIGVALDAAIH
                     SRYFGTGDVGPADIARNLIGSIVKEDLADLQVLKEYMNLVAKKRAASDPAWKTFYDAA
                     VKVLSA"
     gene            complement(8491..>8961)
                     /locus_tag="VGB85_20155"
     CDS             complement(8491..>8961)
                     /locus_tag="VGB85_20155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136413.1"
                     /translation="GTVTQQDRSVQTWEPYLAWQPQAALQRAQSHPPSPLDLPNELQE
                     EVVLPADWTCQEDTARATPERRVFTVAAHGLELDAVVPAGADGSTLAKFFEKRVKRGI
                     DPKRADAKLYGLVHYELGRLVLSPLATLTAKGPEHLMLTGEKINLQALLGSLHL"
BASE COUNT         1345 a         2911 c         3266 g         1439 t
ORIGIN      
        1 cccgacgaat ccgaacggct cgaccgagtc cgacgggccg accggcggcg agacgccgac
       61 gggcggtgag acgccgaccg gcagcggcac ggacactggc ctgaccgatg ggacaggttc
      121 gggcggggcg accgagacgg gcgggcagga cgaggcaggc ggctgcagct gccgttcgga
      181 cgcgccgaac gggctcgcgg gcgggctgct cgggctcggc ctgctcggcc tgcgccggcg
      241 tcgctgatca gagcagcgcg agctgggtgc cctgcgcctg cgcctcgacg cgggcgccga
      301 gatcttgcag gaaccggcgg gcgtccccgg cccactcggg gtcgccgcgc aggaggatct
      361 gcgggcgctc gctggcctcc cagaaggccg cgatcgcggc gcggtcggcc cgcccggggc
      421 acgaggcgcg cgcgacctcg atccgtcgcg cctccggatg ttgcgcgtgc caggcctgga
      481 cctgctggcg tactggttcg ggcgcgtcga tcaacagcac cgcgcccgcg gtctcggccg
      541 gcttgcctct gtgagtaggc cagaagccgg gcgcgttgac ggcgtcgtgc ggccacaggc
      601 gcacgcgcgc cggctcggcg ccggtcgggc gggtgacctt gctgtcgtca ccctcgaggt
      661 gcgggaggaa ccgcgggtgc gcggtgaagg gcacgttacg caggcagcgg tcggcgacca
      721 tgcgcgcgtc gggggcggtg gcgacgagga tgacgacgct tgccgacagc gcgtgcaggg
      781 tctcgatcgc ctcgggcgtg agcgggcggg gcgggtcggg cagcgaccag cacggctcgg
      841 cgcgcagcag cagccactcg cacggcgcgg cgcgggccga ggcgccgagg gcccaggtgt
      901 agaggcagga cggtccgctg ccgggtccgc gaggttgcag gcgcaggctg ctgccgccga
      961 gcgggaggcc ggacgggagg gtacgcagct gcaggcgacc gacgccgaac tcgcggtcgt
     1021 gctcgacgag cacggcgcgc gcgagcgcgg tgcgctgggc cggcgcgagg ttggcgagcg
     1081 cgagcgcgta gatgagatct cgatgcccga tccacggccc gggcacgagg cgcggcgggg
     1141 cgccgacgtg cgacaggaag ctggtcctgc cccgcgcgca cagatcgagg ctcaggcgct
     1201 cgcgggcgat cagctgctgc acgaagtgca gctcgtcctc ggaggtcgcg ctcacgacga
     1261 gacccccggg atcgccgagg ctgtcccgga ggacaggtcg cggcccgacc ggcgcaggcc
     1321 gcggaagaac tttgaaagga cctctccgca cggaccggcg agcacgccgg gcacgacctc
     1381 catgcggtga ttgaggcgcg ggtcgtcgca cagcgagaac aggctgcgcg cggcgccggc
     1441 cttggggtcg gcggcgccgt acaccagccg cgcgacgcgg gcgttgacga gggccccggc
     1501 gcacatcggg cacggctcga gggtgacgta gagggtcgcg ccgtcgaccc gccagcgacc
     1561 ctcacgccgg cacgcctcgc gcagcgcgac gatctccgcg tggccggtcg ggtcggccgc
     1621 ggcctcgcgg gtgttgaagc cgacgccggt gatcgcaccg ttcatcacga cgatcgcgcc
     1681 gaccgggacg tcgccgcggg cggccgcggt cccggcgagg cgcaacgcct cgcgcatgta
     1741 cacgaggtcg aggtcggtcg tcgcgagcat gcgtccggag catcgcgaaa aagcgcaggc
     1801 ggagcaaccg acgcacgccg gtgtccgctg cgcactcgcg gctcagcggg cgagcgcggc
     1861 gaccaggtcg ctggcggcga gtcgtcgcac ctgcaccggc gagaggccgc tcccaccgcg
     1921 gggcgtgacc ctcgctgccg ggtgcggcgg cgaggtcgtc acgcagcaga tccagcagcg
     1981 ccggcgtgct cgccgggaga tcggccctgc gcaagcgcag gcgggccagc gcggtcagca
     2041 gcagcgcggg tgagaccgat caatccggct gctgcgcctc gcgctcgagc acggcgaaca
     2101 cgtcgacctc gggcttgatc ttgtcataca ggtcggggaa ggccgcgtcg gcgccgtact
     2161 tcgcgcggac ctgtcgatac aggtcgagcg agaacatctt ttcaaactcc tcgcgggtca
     2221 tgaaggtgtc ggcgtagagg aacgggtacc cgccgacctc ttgcgtaaat tcctccatct
     2281 tgcgcatcgc gtgcaccgtc ttgaacggct tgccctcgag gatcggcttc gggacgccgt
     2341 agacgccgag gtcgaagaac atcccataat cggtgcccgg cactcggtcc tggtcccgcg
     2401 gcgcgcgcag ctggccctgc aaggcgccat gatcgtagat ccgcgacggg tacaacaata
     2461 cggggtacat ctcgaacagg cggtgcgacg cgtcgatcgc ccggctcatc gcgctcatcg
     2521 gcagcacgat gtcctggaac acctgttttg tgaaagtcat ctcgcgcacg gccggcgtgg
     2581 tcgagaattt gatgaatgcg atccgcggcg gcatcagcca gccgagcagc cagcgaaacg
     2641 ccggatggtt cccgaacggg atcatgtgct gcacgaccca gaagatgctg cgattgtgcc
     2701 gcatgagata atgctcgagc gggatgtact cctcgccgcc ctgctcacgc cacaagaacg
     2761 tctcgacgtg tttaaagaac cacggcttgt accagcggcc gagcgggttg acctcgctgc
     2821 ggtccgcggg ggcgtcggcg aagtcgccgg ccatgatcac ggcgtggtcc ttggcgaaca
     2881 cggtcgcctc gaggaaatcc ggagcgtcgg aggcgaccgc catcgcgtgc agctggtcgc
     2941 agtattgttg ctgcgtgtgc acggggatgt aacgcagccg cacgtgcgac ttgaccggca
     3001 tgatctgtaa ctcgagggca acgagcagcc cgagcgtgcc gtgtgaccac gggagcgcgt
     3061 ggaacagatc cggatgactc tcgcgcgtaa cttttaaaag ggtgccgtcg gcgatcacga
     3121 cctcgtaggc gacgatcgtc tcgaacagca ggccgacctt gtgtgaatgc gtggtcatgc
     3181 cgacggccat cgccaggccg ccgatcgtcg cgtcttcgat ctcgagggtg accgagagca
     3241 tgtggccgcg cggcagcagg tagcgggtgg cctggccgac ggtgacgaag ggctcgacgc
     3301 gaacggtcct gcgccccagg tcgacgccga gcacgttgca caaccccgcc atctggatct
     3361 tgtgccaggc gtgcttcggg gcgaagcggg tgctcaggtt ttgccaggtg cgcctgtcgg
     3421 tgcacattgg tttgcgctgc tcggccggtt gcgcgtacca gcggcggacc tgctcctgaa
     3481 cccggcggac ccgcaggtcg tgctgcgagg ggtcggcgat caggcgctca tatatgaaat
     3541 tgcgcaggcg cagggcggcg tcgaagatgt agccgacggg cagcagcgcg acgaggatga
     3601 cgccgcgaaa gcgcgtcagg aggtcgaaca gaaattgttt catccgcgga tgatctcccc
     3661 catgcagtcg gccagctttt cgggcgtgca cgacaggatg tccatcccga ccttgcgcag
     3721 tttcttggcg gtggcacgat tgtattgcgg ctgggcgtcg tagccgagcg cgccgaggcc
     3781 gatcattcgt acgtcggcgc cagcgagccg ggcgacgacg cggatgaggt cctcctcggg
     3841 gcggccctca taaaagtctg tgatcaacac gatgatcgtg cgcgccggct cgcgcacgag
     3901 gccggagcag tactcgaggc ccttcgtgat atcggtgccg ccgccgagct gtacgctcag
     3961 cagcacgtcg accggggcgc cgacgcggtc ggtcaggtcg acgacctggg tgtcgaacag
     4021 caccagcgag gtcttgacgc cgggcagctc ggcgaagatc gacgccatca cggccgagaa
     4081 gatcgccgac tcgagcatcg agccgctctg gtccacgcag acgatcacgt gccacgggcg
     4141 cttgttcttc tcggcagcgt agaaaaagat gcggtcgacg aacagcttgc gccgctcgtg
     4201 gttccagttg cgcaggttcc ggcgcagggt ggtcttgagg tcgaggttgc ggaacacgcg
     4261 acgcggcgag tgcctatcct tgcgcagcgc gcccgtgatc gactgctcga cgcggatctc
     4321 gagttttttc ttaagatcct gcacgacctt gtcgatgacc ttgcgcgcca agatgcgggt
     4381 cttcggggtc agcaggtcct tgtgggtcag cagggtcttg acgagctcga cgttgggctc
     4441 gatgcggtcc agcaattgcg gctggtcgag gatctgcgcg atgcccttct tctggatcat
     4501 ctcgcgctcg agcacctccc tggcctgctt tggaaacagc tcgttgacct gatcgatcca
     4561 cttcgggacc gtcagctgcg actcctccgc gccgcctccg cgtgactcgc catatacgaa
     4621 cgacagggcc gcctcggcgc ccgcctggtt ctcacccccg cccgcgccgc cgccgggcgc
     4681 gaggccgagg ccgctggcct ccgcgtcctt gccgagcacc agccgccagc gaccgaccag
     4741 cgactgcgac tccgcgggga gctgaccgag cttgaccgtg ttcgccatat ggatctctct
     4801 ctctttttaa aaatcccact ctttcatgac ctcggcgaca cgcgcgtcga tgcgggccat
     4861 ccagaccgcc gcgccggccg aggtgtcgag ctgcgcgagc ttgtcgccct cgccctcttg
     4921 caggccgtag agctgcgcga cgcggtcggc gaaggcgacg cgggaccggt cgtgcaggcg
     4981 ctcgaaggcc ttgcgggtgc gcggcagcat gaccaggaac tggtcccacg aggcggcccg
     5041 cagcagcacg tcgaaggctt ccacaaggga ctgcgcgccg agcatgatcg ccgtgcggct
     5101 ggtcgccatc acgccgtcga ggaactcgcc gcaggtgagc atcacctcgg gccgcgattg
     5161 cgcgaatttg aggatctcct gcgagagaac ctcggccggc tgggcgcggg tctcggtcag
     5221 caggccacac atggcgccgc gcaggtaggg cgagtttgac tgcgcgtagg ccgagcgcac
     5281 gctctcgaca aaaaggtcgc ggtcgaagcc ggcgtcgctg tcgccgagca gggcctcggc
     5341 gatgctcttg agggcggaga gcacgtcctc gtggtcctcg acgggcacgt tggcgacctc
     5401 ggggatcgca aagcaggcgc gcgcgaagca gcggccctgg aggtcgatga cgatgtcctc
     5461 gcggatcttc ttgcgcacgg cctggcggcg cagcacgacc aggtgcgtca gcgcctgcgc
     5521 gagcgagacg agccgcgtgt cggcgtcgat cgcgtggccg gcggtcgcct cgatgcgcag
     5581 cacgaggccg ggcaggtcca tgttggtcgc gcgcagcagg cgggcgcaca cgctggcggc
     5641 gtcgtcttgc tccttcgcca gctcctcgtc gatcttcgcg gcggcggcgg cctccatggt
     5701 gtcaccatag agattgttct cgatcagctg ggtctccacc ttgggtgacc actccaccct
     5761 ccagacctcg cgaaacaggg tgttggtctc gccggtgtcg acgatcgtcg cgaacgagac
     5821 gccgagctcg gcgaggcgat gaaagaacac ggagcggcgc tcgccggcct cctggcggag
     5881 atcgatcgtc agcttgtgct tcttctcctt gcccatcgtc tcgccgaggt cgagctcgtc
     5941 gatcgagcga tagaagtcga agacgagcgg gagccgcccg agcgccgggg tcacgcgacc
     6001 gatcgcggtg ccgatctcga cctccgacat cgcggtcagg agatacttgc cctcctcctc
     6061 ggggcggccc ttgcagcagc acgagagcac gccgtcgcgc acgtcgtcga gcgtcggcgc
     6121 ctgatggccg cgcaggctgg cgagcatgcg cgcgtgctgg gtgatgctga tcgcgtcggc
     6181 cgaggagagc agctcgcccg cgcgacgccc ccgcgagagc accgcgacga cgtggtcgac
     6241 catcgcccgg atcggggcct cctcgggcgc cggaccctgc atgtgctgat ataacatttc
     6301 gtagtacttc ggcgcgcggt tgccggcgcc gtagcctgaa tgctcgctgg tgcgataata
     6361 cgagtagggg gcgacggtcg cgtagaccgt gccggcgggt aagggcttcg cggggaggac
     6421 ctcccggcgc atgaacaggt gaaagccgcc gcacacgacc acggcgtcct ccgggcgcag
     6481 gcgctgctcg ccgaggttcg tcgagatcgc gcgccacatg tgggcctcgc gggcgaaggt
     6541 gccgtcgtgc tgcatgctcg cgtcgctggt ggtcgcgcgc agggcggcgc agaagaacgc
     6601 catgtcgcgg cgaaacgcct cggcgtcgcc gtgccggccg ccgacctcga acaatgcatc
     6661 ccagcactcg gaccacgagc gatagcccgc ggtctccgcg agcactttaa aaaagctgct
     6721 ctcgacggcg aggttctccc aggtcggcgg ctcggcgctc ggggtctcct cggtctcttc
     6781 ttcctcggcg ggtgcatcct cgcggtgggg ttcgatcagg ccgtggtgcg gcaggtcaat
     6841 gaacacgacc tgtgcgccga tctccttcgc gactttgatc gccacgtact cgggggagta
     6901 cgggagcatg gggtaccaca cgccccactt cgccggcacg tccggggcgg agctggcgat
     6961 gccggcgagg ccgagcacgt tgtcgtcgtc gcgatacgac gaatagatcg cgatcggcgg
     7021 cttggtcttg ctgtcctgga tgtgctggac cagctcggtc gcctcggcgg ggccctcgat
     7081 gaagataatc ttcgggcgac gggcgcgtag cgcctcggcg aggtagcgcg cggcggcggg
     7141 cgagtggtgg cgaacgggga accagtacag cggctcgccg agcacctgca tcgcggtcgc
     7201 ctcgacggcg gacgcgtcga ggccgggggt cgcatcgacc gcttcgagga cgttcgcgcc
     7261 ggggtcttgc atcgcttcgc tcaggcggac aggaccttca cggccgcgtc ataaaaggtc
     7321 ttccaggccg ggtcgctggc ggcgcgcttc ttggcgacca ggttcatgta ctccttgagc
     7381 acctggaggt cggcgaggtc ctcttttaca attgacccga tcaggttgcg ggcgatgtcg
     7441 gcggggccga cgtcgccggt gccgaagtat cggctgtgga tcgcagcgtc gagggccacg
     7501 ccgatcgcct cggcggtcga cagcgtggtc gagggctttt gcaggctcag gccctcgccg
     7561 gtggtgccct gccgcatctc gcggaacacg gtggcgagca ggcgcaggac cggctcctcg
     7621 atgcggccgg ggattttgta acgctcgagc agctcggcgg agcgggtgcg aacgatctcc
     7681 acctcggtct tctggtcgga gacgacgggg atgtggatgt agttgaagcg gcgcttgagc
     7741 gcagccgaga gctcgttgac gccctggtcg cgcgcgttgg cggtggcgat gatgttgaag
     7801 ccgggctttg cgaacaccat cgggttgtcg ggcagctcgg gcaccgcgat cgtcttgtcc
     7861 gagcagatcg agaccagcgc gtcctggacg tcgccgacgc agcgcgtgat ctcctcgaag
     7921 cggaagatct tgccggcgcg catcgccagc atgcacgggc tctggatcat gttgtcgggc
     7981 ttgggaccct cggcgatgat ccgcgcgatg ttccaggagt atttgatctg ctcctcggtg
     8041 gtgccggcgg tgccctggat cgtcagcagc gaggtgccgg agatcgcggc ggcgaggtgc
     8101 tcggacagcc agctcttgcc ggtgccgggg tcaccgacca acagcagcgc gcgctcgctg
     8161 gcgagcgtga cgatcgaccg ctcgacgatc gagtcgtcgc cgaagaattt tctcgtgatc
     8221 gtgacctcga cgaccttgcc gtcgatcgtc gccttgagcg gcttcttgcc gccgacgatg
     8281 taagtcagca ccgagcgcgg cgagagcttc caggcggccg gcgcctggtc ggcctcgttt
     8341 tggcgcaggg cctcgagctc gcggcgatac ttgacctcgg cgggctcacg cagcagctcg
     8401 gattcggggg ccacgggtgc ggagcccggc tcgggcggtg cgctgacgtc ggcgggggtg
     8461 gtcttcgctt tggccatggt cgtgtcttct ttagaggtgc aggctgccga gcagcgcctg
     8521 gaggttgatc ttctcgccgg tgagcatcag gtgctccggg cccttggcgg tcagcgtggc
     8581 gagcgggctg agcaccaggc ggccgagctc gtagtgcacc aggccgtaga gcttcgcgtc
     8641 cgcccgtttc gggtcgatgc cacgcttgac gcgtttctcg aagaacttcg ccaacgtgct
     8701 accgtcggcg ccggccggca cgacggcgtc gagctcgagg ccgtgcgccg cgacggtgaa
     8761 cacgcggcgc tccggggtgg cgcgcgcggt gtcctcctgg caggtccagt cggcgggcag
     8821 cacgacctcc tcctgcagct cgttgggcag gtcgagcggg ctcggcgggt ggctctgtgc
     8881 gcgctgtaat gcggcctgcg gctgccacgc gaggtagggc tcccaggtct gcaccgatct
     8941 gtcctgctgg gtgacggtgc c
//
LOCUS       DASWNJ010001196         1585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_404326, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001196 DASWNJ010000000
VERSION     DASWNJ010001196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404326"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="VGB85_20160"
     CDS             complement(<1..1029)
                     /locus_tag="VGB85_20160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136414.1"
                     /translation="MDEDRPTLSYRTPAAAAPDAAPTPSEQLADLTVPMLGQVSVELG
                     LGAPVRLSLDREELRLTPGSGVRVRAAGLPPLLLRHLRIDLGTAAFEHDATGLGAFEA
                     RAVSVALGAAFTHLAPWQPGRSIVDLVMQNLPQRADGRRRVWGAGPAGVWVDPDTRLD
                     LAVTPEALELALSHAVYLRVIGLGIGLLAVRYLFGSQRLEIEGPRGQPLRNALLRFVA
                     WVATRYLRKRLPPALATPGYDPFADPDRRAHFQQLAAAFAAKQPAPAPSDSQPAATST
                     GDAVDLTAAAKPTDTLPTDAVTAGAATGITRDPAAATARITDAARPTATATAPIETSA
                     RGPATATTT"
     gene            complement(1214..>1585)
                     /locus_tag="VGB85_20165"
     CDS             complement(1214..>1585)
                     /locus_tag="VGB85_20165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136415.1"
                     /translation="PGAWRPGSRAICEAELAEIPGGARLVVRFRMHPLTRSAFIFLTV
                     IGLAMVGFQLIVAGPATAAVLLVPILVVTSILAADRGRLRRQQAALRSLIESTCTPLA
                     QPRAPAPEDPFRLTALPGTDR"
BASE COUNT          198 a          496 c          706 g          185 t
ORIGIN      
        1 ggtggtcgtc gcggtcgcgg ggccccgcgc ggaggtctcg atcggggcgg tcgcggtcgc
       61 ggtcgggcgc gcagcgtcgg tgatccgcgc ggtcgcggcg gcggggtcgc gggtgatgcc
      121 ggtcgccgca ccggcggtga cggcgtcggt gggcagcgtg tccgtgggct tcgcggcggc
      181 ggtgaggtcg acggcgtcgc cggtgctcgt cgcggcgggc tggctgtccg aaggggcagg
      241 tgcgggctgc ttcgcggcga aggcggcggc gagctgctgg aagtgggcgc ggcggtcggg
      301 gtcggcgaag gggtcgtagc cgggcgtggc gagggccggg ggcaggcgct tgcggaggta
      361 gcgggtggcg acccaggcga cgaagcgcag cagggcgttg cgcagtggtt ggccgcgcgg
      421 gccctcgatc tcgaggcgct ggctgccgaa caggtagcgc acggcgagca ggccgatgcc
      481 gaggccgatc acgcgcaggt agacggcgtg cgagagggcg agctcgaggg cctcgggggt
      541 gacggcgagg tcgaggcggg tgtcggggtc gacccacacg ccggccggtc cggcacccca
      601 cacgcggcgg cgcccgtcgg ctcgctgcgg gaggttctgc atcacgaggt cgacgatgct
      661 gcgaccgggc tgccacggcg cgaggtgggt gaaggcggcc ccgagggcga ccgagacggc
      721 gcgcgcctcg aaggcgccga ggccggtggc gtcgtgctcg aaggcggcgg tgccgaggtc
      781 gatgcggagg tggcggagca gcagcggcgg gagcccggcg gcgcgcacac gcacgccgct
      841 gccgggggtg aggcgcagct cctcgcggtc gaggctgagg cggaccggtg cgccgagccc
      901 gagctcgacg ctgacctggc cgagcatcgg cacggtgagg tcggcgagct gttcgctggg
      961 cgtcggcgcg gcgtcgggcg cggcggcggc gggggtgcgg tagctcaggg tcggccgatc
     1021 ttcatccatg acctccgttt aatggatctg gcgcgatgtg acgggtcagg gccgcgacgg
     1081 ggcggcgaga ggcggtcgtc gaggacgatc gccggggtcg atccgcgatc gcctcgaccg
     1141 cgccggagaa gccgtggcgg accactggac ggcgttttgg acgatctcgc ggcggcgccc
     1201 cggagtcgac ggatcagcgg tcggtgccag gcagggcggt gaggcggaag gggtcctcgg
     1261 gcgccggggc gcgcggctgc gcaagtggcg tgcaggtcga ctcgatcagc gaccggagcg
     1321 ccgcctgctg cctgcgcaga cgcccgcggt cggcagcgag gatcgaggtc acgacgagga
     1381 tcggcaccag caacacggcc gcggtcgccg gcccggcgac gatcagctgg aagccgacca
     1441 tcgccaggcc gatgacggtc aggaagatga aggcgctgcg cgtcagcggg tgcatgcgaa
     1501 accggaccac cagacgcgcg ccgccgggga tctcggcgag ctccgcctcg cagatcgccc
     1561 gcgagccggg ccgccacgcc cccgg
//
LOCUS       DASWNJ010001197         2892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_514269, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001197 DASWNJ010000000
VERSION     DASWNJ010001197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514269"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2892
                     /locus_tag="VGB85_20170"
     CDS             <1..>2892
                     /locus_tag="VGB85_20170"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0136416.1"
                     /translation="RAVAPRLAAVVLRLLAKNAEDRPQDTSEVLAALAEHDEHGAAPQ
                     QFRLPQKLYGRETASAELIAGFQRAAAGACVFALVAGYAGVGKSSLVLELHRPLTASR
                     GFFASGKFDQYNRGVPFAAVITAAATLLQHVLTLGAREIDGWRERIAHAVGGALPVLA
                     QIVPDVQAIVGPQPEPPPLPTTEAQQRTQSTLQRFIGVFAAEEHPLVLFLDDLQWSDG
                     ATMRLLEALVTDPGAHHLLLLAAYRDHELDPGHRLAALLAELRNGAAPPLEIRLGPLT
                     TDDVAALLRDTLATPNVDDLARELVHRTHGNPLFIREFLRFLHRESLLRHDEARNAWT
                     WDLTSVDAARIPDSVAALLGDELRRLPQGSQEVLQLASCLGTRFDAGTLALVHGDDVA
                     ALARLLQPAIDLDLIIPLHPSYRLLAHAEAAELELSVPLRFLHDRVRQAAYALIPADE
                     RPARHALVGRRLLARARAEDQLHEALFDVVSHLNEATALLDADELDDLAALDLAAARR
                     AKASAAYDTALILLQAGITALGPDAWDRRRDLSFALCSEQAECEYLSGAPDRALERLE
                     QIEPHAQDSVQRLQLADLRVVLYASMGDTRGALQAGLAGLAAAGVEIPGDDAACRAEV
                     AAELARIEADLARAPLDALVEAPPLADPKIRGVLKLLADMLAPANMTRPDLYALINTK
                     QIRLSIEHGHTDISAYTYVVYGYFLATVLRQYAQADLFGRFALQLNDRLGNSALRCRL
                     RFVFATYAHFTRPLRGVLAEFVGAQDDGRESGDHIYRASACSHVLISRIGLGDPLSEV
                     AEQADRFIALMQRMRVASSAAAQTVSRQFTAALQGHTRAAYSLDDDGFDEATFIVRAE
                     RGGLTFALRWYATVKLQLCLLFDRQADARALLDRFAGAIASNFAFYFTTEFAFYAALM
                     LLRLAGEADPAERPALLAAHAPYADALAGWAAACPDDHANNYAHKAALVR"
BASE COUNT          372 a         1221 c          900 g          399 t
ORIGIN      
        1 cgcgccgtcg ctccgcggct cgccgccgtc gtcctgcgcc tgctcgccaa gaacgccgag
       61 gaccgcccgc aggacaccag cgaggtgctc gccgcgctcg ccgagcacga cgagcacggc
      121 gccgccccgc agcagtttcg cctgccacaa aagctctacg gccgcgagac cgccagcgcc
      181 gagctgatcg ccggcttcca gcgcgcggcc gccggcgcct gcgtgttcgc gctcgtcgcc
      241 ggctacgcgg gcgtcggcaa gtcgtcgctc gtgctcgagc tgcaccgccc gctgaccgcc
      301 agccgcggct tcttcgcgtc cggcaagttc gatcaataca accgcggcgt cccgttcgcg
      361 gcggtcatca ccgccgccgc gaccctgctg cagcacgtgc tgaccctcgg cgcgcgcgag
      421 atcgacggct ggcgcgagcg catcgcccac gccgtcggcg gcgcgctgcc ggtgctcgcc
      481 cagatcgtgc ccgacgtgca ggcgatcgtc ggcccgcagc ccgagccgcc gccgctgccg
      541 accaccgagg cccagcagcg cacccagtcg accctgcagc gcttcatcgg cgtgttcgcg
      601 gccgaggagc acccgctcgt gctgttcctc gacgacctcc agtggtccga cggcgcgacg
      661 atgcggctgc tcgaggcgct cgtcaccgac cccggcgcgc atcacctgct gttgctcgcg
      721 gcctaccgcg accacgagct cgaccccggc caccgcctcg ccgccctgct cgccgagctg
      781 cgaaatggcg ccgcgccgcc gctcgagatc cggctcgggc cgctgaccac cgacgatgtc
      841 gccgccctgc tgcgcgacac cctcgccacg cccaacgtcg acgacctcgc gcgcgagctg
      901 gtccaccgca cccacggcaa cccgctgttc atccgcgagt tcctgcgctt cctccaccgc
      961 gagagcctgc tgcgacatga cgaagcgcgc aacgcctgga cctgggacct gaccagcgtc
     1021 gacgccgcga ggatccccga cagcgtcgct gccctgctcg gcgacgagct gcgccggctg
     1081 ccgcagggct cgcaggaggt gctgcagctg gcctcctgcc tcggcacgcg cttcgacgcc
     1141 gggaccctcg cgctcgtgca cggcgacgac gtcgccgcgc tcgcgcgcct gttgcagccc
     1201 gcgatcgacc tcgacctgat catcccgctg cacccgagct accgcctgct cgcgcacgcc
     1261 gaggccgccg agctcgagct ctcggtcccg ctgcgcttcc tccacgaccg cgtgcgccag
     1321 gccgcctacg cgctgatccc cgcggacgag cggcctgcca ggcacgcgct cgtcggtcgc
     1381 cgcctgctcg cccgggcgcg cgccgaggac cagctgcacg aggcgctgtt cgacgtcgtc
     1441 tcccacctca acgaggcgac cgcgctgctc gacgccgacg agctcgacga cctcgccgcc
     1501 ctcgacctcg ccgccgcgcg ccgggccaag gcgagcgccg cctacgacac cgcgctgatc
     1561 ttgcttcagg ccggtatcac cgcgctcggc cccgacgcct gggaccggcg gcgcgacctg
     1621 agcttcgcgc tgtgctccga gcaggccgag tgcgagtacc tcagcggcgc ccccgaccgc
     1681 gcgctcgagc ggctcgagca gatcgagccg cacgcgcagg acagcgtgca acggctccag
     1741 ctcgccgacc tgcgcgtggt cctgtacgcg agcatgggcg acacccgcgg cgccttgcag
     1801 gccggcctcg ccgggctcgc ggccgccggc gtcgagatcc ctggggacga cgcggcgtgt
     1861 cgggccgagg tcgccgccga gctcgcgcgc atcgaggccg acctcgcccg cgccccgctc
     1921 gatgcccttg tagaagcccc accgctcgcc gacccgaaga tccgcggcgt gctcaaattg
     1981 ctcgccgaca tgctggcccc ggccaacatg acgcgccccg acctctacgc gctgatcaac
     2041 accaagcaga tccgcctgtc gatcgagcac ggccacaccg atatctccgc ctatacatac
     2101 gtcgtatacg gctacttcct cgccacggtc ctgcgccagt acgcgcaggc cgacctgttc
     2161 ggccgcttcg ccctccagct caacgacagg ctcggcaact ccgcgctgcg ctgccgcctg
     2221 cgcttcgtct tcgccaccta tgcccacttc acccgcccgc tgcgcggcgt gctcgccgag
     2281 ttcgtcggcg cccaggacga cggccgcgag tccggcgacc acatctatcg cgcctccgcg
     2341 tgcagccacg tcctcatctc ccgcatcggc ctcggcgacc cgctctccga ggtcgccgag
     2401 caggccgacc gcttcatcgc cctgatgcag cgcatgcgcg tcgcctcctc ggccgccgcg
     2461 cagaccgtct cgcgtcagtt caccgccgcc ctgcagggcc acacgcgcgc cgcgtacagc
     2521 ctcgacgacg acggcttcga cgaggccacc ttcatcgtcc gggccgagcg cggcggcctg
     2581 accttcgcgc tgcgctggta cgcgaccgtc aagctccagc tgtgcctgct gttcgaccgc
     2641 caggccgacg cgcgcgcgtt gttggatcgc ttcgcgggcg cgatcgccag caacttcgcg
     2701 ttttacttca ccaccgagtt cgccttttat gccgcgctga tgctgctgcg cctcgccggc
     2761 gaggccgacc cggccgagcg ccccgcgctg ctcgccgccc acgcgcccta cgccgacgcg
     2821 ctcgccgggt gggccgccgc ctgccccgac gaccacgcca ataactacgc ccacaaggcc
     2881 gccctcgtgc gc
//
LOCUS       DASWNJ010001198         1537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_126639, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001198 DASWNJ010000000
VERSION     DASWNJ010001198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126639"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..337
                     /locus_tag="VGB85_20175"
     CDS             95..337
                     /locus_tag="VGB85_20175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136417.1"
                     /translation="MSVVRGLVLLHLSATALLLLVLVAVMACQRAADVVRSRRALRGA
                     TAVAYPQEAGASVLDWTIDLRTPVAADGGAVLQRTS"
     gene            425..>1537
                     /locus_tag="VGB85_20180"
     CDS             425..>1537
                     /locus_tag="VGB85_20180"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0136418.1"
                     /translation="MSNVAGVRGSASGDDARDRSGREVAELQAQVGYLEQEVSVLRRK
                     LADSPRQVRALEEGLTQAQASLAAVTGQNDRLVGTLREARDQILALKEEVERLAQPPS
                     GYGVFLTRHDDDTVDVFHGGRKMRVAVSPSVELDTLRRGQEVMLNEAMNVVLALEFER
                     QGDVVMLKELLDGEDGTRDRALVIGHTDDERVVMLADSLKDQPLKAGDSLLLETRSGY
                     AYERIPKSEVEELVLEEVPDIDYGDIGGLAAQIEQIRDAVELPFLHKDLFKEHQLRPP
                     KGVLLYGPPGCGKTLIAKAVANSLAKKVAEVTGKPDGRAYFLNIKGPELLNKYVGETE
                     RHIRLIFQRAREKASEGTPVIVFFDEMDSIFRTRGSG"
BASE COUNT          238 a          550 c          543 g          206 t
ORIGIN      
        1 aagaagtgtg cgacgacgcg acccgtggct cgtcctgact agtgaggcga ccgtctctga
       61 ccgttgacgg tcctcgaaca ccctggagat gcccgtgtcc gtcgtccgtg gcctggtgct
      121 gctgcacctc agcgccaccg cgctcctgct cctcgtcctc gtggccgtca tggcctgcca
      181 gcgggccgcg gacgtggtgc gctcccgccg ggccctgcgc ggcgcgaccg ccgtcgccta
      241 cccgcaggag gccggcgcgt ccgtcctcga ctggaccatc gacctgcgca cgccggttgc
      301 ggcggacggc ggcgccgtgc tgcagcggac ctcctgacag cagccggcct tcggcccgtc
      361 ggacggcgca gtcgcgcacc gcgaccgtag gatgccgagg gagcaggatc ccctcagacg
      421 ggtggtgagc aacgtggcag gagtgcgcgg gagcgcctcg ggcgacgacg cccgggaccg
      481 gagcggccgc gaggtcgccg agctgcaggc gcaggtcggc tacctcgagc aggaggtctc
      541 ggtcctgcgg cgcaagctgg ccgactcgcc tcgccaggtg cgggcgctcg aggaggggct
      601 gacgcaggcg caggccagcc tggcggcggt gacgggccag aacgaccggc tcgtcggcac
      661 cctgcgggag gcgcgcgacc agatcctcgc gctcaaggag gaggtcgagc gcctcgcgca
      721 gccgccgagc ggctacggcg tcttcctcac ccggcacgac gacgacaccg tcgacgtctt
      781 ccacggcggc cgcaagatgc gcgtggcggt gagcccgtcc gtcgagctgg acacgctgcg
      841 gcgcggccag gaggtcatgc tcaacgaggc gatgaacgtc gtgctcgccc tcgagttcga
      901 gcgccagggc gacgtcgtca tgctcaagga gctgctcgac ggcgaggacg gcacccgcga
      961 ccgcgcgctg gtcatcggcc acacggacga cgagcgcgtc gtcatgctgg ccgactcgct
     1021 caaggaccag cccctcaagg ccggcgactc gctgctgctc gagacgcgca gcggctacgc
     1081 ctacgagcgg atccccaagt cggaggtcga ggagctcgtc ctcgaggagg tccccgacat
     1141 cgactacggc gacatcggcg gcctggcggc gcagatcgag cagatccgcg acgccgtcga
     1201 gctgccgttc ctgcacaagg acctgttcaa ggagcaccag ctccgcccgc cgaagggcgt
     1261 gctgctctac gggccgccag ggtgcggcaa gaccctcatc gccaaggcgg tcgcgaacag
     1321 cctggccaag aaggtcgccg aggtcaccgg caagcccgac ggccgcgcgt acttcctcaa
     1381 catcaagggc cctgagctcc tcaacaagta cgtcggcgag accgagcggc acatccggct
     1441 catcttccag cgggcccgcg agaaggcgtc tgagggcacg cccgtcattg tcttcttcga
     1501 tgagatggac tcgatcttcc gcacccgcgg ctcgggc
//
LOCUS       DASWNJ010001199         8974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_553121, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001199 DASWNJ010000000
VERSION     DASWNJ010001199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 8974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8974
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553121"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VGB85_20185"
     CDS             complement(<1..723)
                     /locus_tag="VGB85_20185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136419.1"
                     /translation="MLAIGCATERGRGWDTPARAPSAPAVSLVLLGEVGHPGRTAGHV
                     AAEVRRTLAVRRAAGVPVILLWLGDMLGDTCEPTASLGRPGVRAFAELAREHAAAGGR
                     GYAVLGAHEWRCGAPSVHVQAEAGPRPWRMPAANYVVRVARDGASTVVSKCSERLCTV
                     EPAADGALVDLVLLDTAAWLGPAARGGPLLAAADASLVQQQALLAAIEATPSSVPRLL
                     VTHHPIETAGPHGQGGLFPDSAY"
     gene            1059..1559
                     /locus_tag="VGB85_20190"
     CDS             1059..1559
                     /locus_tag="VGB85_20190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY0136420.1"
                     /translation="MSHLLLSMTVNGEPVQVAAPAHHTLLEVLRYKLDLIGTKQGCDK
                     GDCGACTVQVDGVPQLSCLTLAAHAEGRSVTTVEGLGMQAGGCHPLQDAFDRWDAAQC
                     GFCTPGILMSAEALLRRTAGRPLTRQAVCEALAGNLCRCTGYTKIIDAVLDVGQQQTT
                     EVSRAD"
     gene            1549..3924
                     /locus_tag="VGB85_20195"
     CDS             1549..3924
                     /locus_tag="VGB85_20195"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY0136421.1"
                     /translation="MPIEDRERDWQLGKSHRKVDAMERMRGVTRYTDDLKLPGMLHAK
                     ILRSPHAHARIKSIDLAPALAIPGVFAAVTGQDFKIPYGIIPWTQDENALAVDKVCHV
                     GDGVVAIAAIDEDTANAACKAVRIEYEVLRAFFDPEEALKVRDRDGTINPYARKGNLS
                     KHVLLEFGEVDAELASSDLVVEGEYHFEGTTHAAIEPHCALAHVETNGILTVWSATQV
                     SHYLHRELSKVLELPPHRIRVIQPPLGGAFGGKSEPFDLEFCVAKLAMITRRPVKLLY
                     TREEVFYAHRGRHPMKMKFRTGFARDGKIKAVESKTILDGGAYSSFGLVTTYYSGQLL
                     CAPYGFGTYRFDSTRAYTNKPACGPKRGHGSVQPRFAMEIQLDKAAVALGIDPIELRR
                     RNDIGSNTQTVNEFRVGSNGFLECLDKVEAASGWRERRATLPYGRGLGVAGSTYISGT
                     NYPIYPNEMPQAGVQICLDRSGRARVFSGANDIGQGSNTMLAVIVSEELGLPLEDIRV
                     LSADTDLCPVDLGAYSSRITLMVGNACLAAAQKLRSKVCESVAKRWKVPKNRVVLAGR
                     MAIDREDPERNISSAEAFVWAEVDHGLLGEVGSYNTPKDRHGEYRGGTIGASPAYSFT
                     AHLAEVEVDPETGIVDVKKIWVAHDCGKALSKRIVEGQIEGSTYMGFGEALMEEHLVD
                     SKHRGAHAGPSLLDYRIPTFLDTPEIAAMIVEAPDANGPYGAKEAGEGPLHSTIPAIA
                     NAIYDAVGIRLDRLPFTPARVLAAIQERQRREQAGELAPHKPVHDVERKIA"
     gene            3924..4928
                     /locus_tag="VGB85_20200"
     CDS             3924..4928
                     /locus_tag="VGB85_20200"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEY0136422.1"
                     /translation="MLRLPQFEVESPDTVEGVVNALQRRPGARLIAGGTDILPNLKHF
                     LDTPPALISLARVTALRGVVRDDAAGVLRIGASVTLAGVSEHPEVRALFPSLADAAGS
                     VATPLIRNMGTLGGNVNLDTRCRYVNQSEFWRASIGGGCLKSEGNVCHVVPKGRNCVA
                     AMSSDNVPVLIGLGASIVLVGPDGRRELPLEEYYTADGTAHTLAKPGEMTVELRVPLP
                     QGPRRTKYVKWSVRKSVDYPLLSVALRFDLATDSAHGEVTGVYVVCGVLGSKPRVVTR
                     LDEVLGKKLSDAAVARIVSEATYAQCKPLENVPYEAAYRREMLRVYTRRAVEAMVAGG
                     "
     gene            4937..5707
                     /locus_tag="VGB85_20205"
     CDS             4937..5707
                     /locus_tag="VGB85_20205"
                     /inference="COORDINATES: protein motif:HMM:NF017551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HEY0136423.1"
                     /translation="MCTLLLAWQVDPARPLVVAANRDEFYARPSERAAFWPDAPQVLA
                     GRDLEGGGTWLGVTRDGRFAALTNVREPGAPTPPQAPSRGRLVAEFLLARGAPDEYLA
                     GLRPADYAGFNLVVGDGRSLWYLSNRAGPARALGPGVYGVSNAALDTPWPKLLRGRER
                     LAALVAGGADLPALLEMLEDRSPTADAELPDTGVGLAIERMLSPLFIASPAYGTCSST
                     ALVVHRDGALELRERTQNPLAPAGPGEVAYALAAPPAA"
     gene            5819..6442
                     /locus_tag="VGB85_20210"
     CDS             5819..6442
                     /locus_tag="VGB85_20210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136424.1"
                     /translation="MSAAALHASVAHAADGDKPKPAWRTEKHILEVGGYLGALFPAAD
                     HGLYAENITKTQRPIKTGFDVGIRFAYLPLRFVGVEVEGGIVPTKLDGNGGYKTTLFA
                     VRGHLILQMPTRLALFVVGGGGILGVTSSAKGLGSNVDGTLHVGGGLKYYLTEKVVLR
                     IDGRDIASPNFSRGMAGGQDWAHNGEFTFGASFVIGRKSTKMLPRGG"
     gene            7537..8895
                     /locus_tag="VGB85_20215"
     CDS             7537..8895
                     /locus_tag="VGB85_20215"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0136425.1"
                     /translation="MPGGVLKRRTLRSNANGAVDLSGQVLGGRYRLLRPLGQGGMAII
                     YEAEHVAIGKRVALKVLAQARCSATVQRFLMEARAAAELRHEHIIDVTDFGQDTLADG
                     EPVAFLAMEYLEGEDLAATLEQDGPLRWTRVLAIGKQICRALIAAHDQGIVHCDIKPA
                     NCYRIQRGETADYIKVLDFGVASFACKRTGRCTPDPIPAPEGGRPRSGPRIGTPGYMA
                     AELLRGGSYDHRVDIYALGVLMYRLLTNKMPYPAAKLYAAASDSSPGHALARPASATG
                     ESSGMTPVAPFPMQRAIPTLEISPAFEAVVLQAIAHDPEQRQPNARALYEALVAAEQA
                     SMRGSLLPRDPLCWGATREVPRADDSSSMSQTEVSRPAFAASGLSNAQLRRTAPDLGP
                     GSSHASQASQASSAASGAWRPTTRPAMPPLAVSPFGLLVRATLALGVTTAVVRAAWAL
                     VA"
BASE COUNT         1338 a         3131 c         3266 g         1239 t
ORIGIN      
        1 gtaggcggag tcggggaaca ggccgccctg accgtgcggg ccggcggtct cgatcggatg
       61 atgcgtgacg agcaggcgcg gcacgctcga cggcgtcgct tcgatcgcgg ccagcaacgc
      121 ctgctgctgg accaggctgg cgtcggcggc ggccagcagc gggccaccac gcgcggccgg
      181 gccgagccac gcggcggtgt cgagcagcac gaggtcgacg agcgcgccgt cggcggcggg
      241 ctcgacggtg cacaggcgct cgctgcactt cgacacgacg gtgctggcgc cgtcgcgggc
      301 gacgcggacc acgtagttgg cggccggcat gcgccacggg cgggggccgg cctcggcttg
      361 cacgtgcacc gagggcgcgc cgcagcgcca ctcgtgggcg ccgagcaccg cgtagccgcg
      421 cccgccggcg gccgcgtgct cgcgcgcgag ctcggcgaag gcgcggacgc cgggccggcc
      481 gagggacgcg gtgggctcgc aggtgtcgcc gagcatgtcg ccgagccaga gcaagatcac
      541 cggcacgccg gcggcgcggc gcaccgcgag cgtgcggcgg acctcggcgg cgacgtgtcc
      601 ggcggtgcgc ccggggtggc cgacctcgcc gagcagcacc aggctgaccg cgggcgcgga
      661 cggggctcgc gcgggggtgt cccagccgcg gccacgctcg gtcgcgcagc cgatcgccaa
      721 catggccgcg aggacaggta cgagccggcg catcacaggg tgtccgtccc ccggtcgagg
      781 cacgcgcgcg cgtcgtgcgg gtcggcgagg ccctcgagcg gtcgccgggc gaccggagtt
      841 cgtcgggtgg agcgcatggg cggtcgcgcg agcctaccac gccggcccgt gagatcggcc
      901 gcgctacgcg ccacggaggg tcggcgcgtc aagttcacgc ggggggccgg tggctggcgt
      961 ggacccgcga attcccgggc tgcggcgagc tttcgcacgg tttccgggcg cgtgggcgct
     1021 tgcacccatg gaggcgctcg ggcaatgctt tgcgaacgat gtctcatctg ctgctgtcga
     1081 tgaccgtcaa cggcgagccc gtgcaggtcg cagcgccggc gcaccacacg ttgctggagg
     1141 tgctgcgcta caagctcgac ctgatcggca ccaagcaggg ctgtgacaag ggcgactgcg
     1201 gggcgtgcac ggtgcaggtc gacggcgtgc cgcagctcag ctgcctcacg ctggcggccc
     1261 acgccgaggg ccgctcggtg acgacggtcg agggcctcgg gatgcaggcc ggcggctgtc
     1321 acccgttgca ggacgccttc gaccgctggg acgcggcgca gtgcgggttc tgtacgccgg
     1381 ggatcctgat gagcgccgag gccctgctgc gccgcaccgc gggtcgaccg ctgacgcggc
     1441 aagccgtgtg cgaggcgctg gccggcaacc tgtgccgatg cacgggctac accaagatca
     1501 tcgacgccgt gctcgacgtc ggacaacagc agacgacgga ggtgtcgcgt gccgattgaa
     1561 gaccgcgagc gcgactggca gctcggtaaa tcccaccgca aggtggacgc gatggagcgg
     1621 atgcggggcg tcacccgcta caccgacgac ctcaagctgc cggggatgct tcatgccaag
     1681 atcctgcgca gcccgcacgc gcacgcccgg atcaagtcga tcgacctcgc cccggcgctg
     1741 gcgatccccg gcgtgttcgc ggcggtgacc ggccaggact tcaagatccc gtacggcatc
     1801 atcccgtgga cgcaggacga gaacgcgctc gcggtcgaca aggtgtgtca cgtcggcgac
     1861 ggcgtggtcg cgatcgcggc gatcgacgag gacacggcga acgcggcctg caaggccgtg
     1921 cgcatcgagt acgaggtgct gcgcgccttc ttcgaccccg aggaggcgct caaggtccgc
     1981 gaccgcgacg gcacgatcaa cccctacgcg cgcaagggca acctctcgaa gcacgtgctg
     2041 ctcgagttcg gcgaggtgga cgcggagctg gcgagctcgg acctcgtcgt cgagggcgag
     2101 tatcacttcg agggcaccac gcacgcggcg atcgagccgc actgcgccct cgcgcacgtc
     2161 gagaccaacg ggatcctcac ggtgtggtcg gcgacgcagg tgtcgcacta cctgcaccgc
     2221 gaattatcca aggtgctcga gctcccgccg caccggatcc gcgtgatcca gccgccgctc
     2281 ggcggggcct tcggcggcaa gagcgagccc ttcgacctcg agttctgcgt cgccaaattg
     2341 gcgatgatca cgaggcggcc ggtcaagctg ctgtacaccc gcgaggaggt gttctacgcc
     2401 catcgcggtc gccacccgat gaagatgaag tttcgcaccg gcttcgcgcg ggacggcaag
     2461 atcaaggcgg tcgagtcgaa gacgatcctc gacggtgggg cctactcgag cttcggcctg
     2521 gtgacgacct actactcggg ccagctgctg tgcgcgccgt acggcttcgg gacctaccgc
     2581 ttcgacagca cgcgcgcgta caccaacaag ccggcgtgcg gacccaagcg cgggcacggc
     2641 tcggtgcagc cgcgcttcgc gatggagatc cagctcgaca aggccgcggt ggcgctcggc
     2701 atcgacccga tcgagctgcg ccgccgcaac gacatcggca gcaacacgca gacggtcaac
     2761 gagttccgcg tcggatccaa cggcttcctc gagtgtctgg ataaagtcga ggcggcctcg
     2821 gggtggcgtg agcggcgggc gacgctgccg tacgggcgcg ggctcggggt cgcgggctcg
     2881 acctacatct cgggcacgaa ttatccgatc taccccaacg agatgccgca ggccggcgtc
     2941 cagatctgcc tcgaccgcag cggtcgcgcg cgggtgttct ccggcgccaa cgacatcggc
     3001 cagggcagca acacgatgct cgcggtgatc gtcagcgagg agctcgggct gccgctcgag
     3061 gacatccgcg tgctctcggc ggacaccgat ctgtgcccgg tcgacctcgg ggcgtacagc
     3121 tcgcgcatca cgctgatggt cggcaacgcc tgcctggcgg cggcgcagaa gcttcgcagc
     3181 aaggtgtgtg agagcgtggc gaagcgctgg aaggtgccca agaaccgggt cgtgctggcc
     3241 ggtcgcatgg cgatcgaccg cgaggatccg gagcgcaaca tctcgagcgc ggaggccttc
     3301 gtgtgggcgg aggtcgacca cggcctgctt ggcgaggtcg gcagctacaa cacaccgaag
     3361 gaccgccacg gcgagtaccg cggaggcacg atcggcgcgt cgccggcata cagcttcacg
     3421 gcgcacctcg ccgaggtcga ggtcgacccg gagaccggca tcgtcgacgt caagaagatc
     3481 tgggtcgcgc acgactgcgg caaggcgctg agcaagcgca tcgtcgaggg gcagatcgaa
     3541 ggctcgacgt acatgggctt cggggaggcg ttgatggagg agcacctcgt cgacagcaag
     3601 caccgcggcg cgcacgcggg cccgagcctg ctcgactatc gcatcccgac cttcctcgac
     3661 acgccggaga tcgcggcgat gatcgtcgag gcgccggacg ccaacgggcc gtacggggcc
     3721 aaggaggccg gtgaggggcc gctgcactcg acgatcccgg cgatcgccaa cgcgatctac
     3781 gacgcggtcg gcatccgcct cgaccgcctg ccgttcacgc ccgcgcgggt gctcgcggcg
     3841 atccaggagc gccagcgccg ggagcaggcc ggcgaattgg cgccacacaa gcccgtacac
     3901 gacgtcgaga ggaagatcgc ctgatgctgc gcctcccgca attcgaggtc gagagccccg
     3961 acacggtcga gggcgtggtc aacgcgttgc agcggcgacc cggggcgcgg ctgatcgccg
     4021 gcggcaccga catcctgccc aacctcaagc acttcctcga cacgccgccc gcgctgatca
     4081 gcctcgcgcg ggtcacggcg ctgcgtgggg tcgtgcgcga cgacgcggcc ggggtgctgc
     4141 ggatcggcgc gagcgtgacg ctcgcggggg tctcggagca cccggaggtc cgcgcgctgt
     4201 tcccgagcct cgccgacgcg gccggctcgg tggcgacgcc gctgatccgc aacatgggca
     4261 cgctcggcgg caacgtgaac ctcgacacgc gctgccgcta tgtcaaccag tccgagttct
     4321 ggcgcgcgtc gatcggcggc ggctgcctca agtcggaggg caacgtgtgc cacgtggtgc
     4381 ccaagggccg caactgcgtc gcggcgatga gcagcgacaa cgtgccggtg ctgatcgggc
     4441 tcggggcgag catcgtgctc gtcggcccgg acggccgccg cgagctgccg ctcgaagaat
     4501 attacacggc cgacggcacc gcccacacgc tggcgaagcc gggcgagatg accgtcgagc
     4561 tgcgtgtgcc gctgccgcag ggaccccggc gcacgaagta cgtcaagtgg tcggtgcgca
     4621 agagcgtcga ctacccgctg ctctcggtcg cgctgcgctt cgacctcgcg acggactccg
     4681 cgcatggcga ggtgaccggg gtatacgtgg tgtgcggcgt gctcggctcc aagccgcgcg
     4741 tggtcacccg gctcgacgag gtgctcggca agaagctgtc ggacgcggcg gtcgcccgca
     4801 tcgtcagcga ggccacctac gcgcagtgca agccgctgga gaacgtgccc tacgaggcgg
     4861 cgtatcgcag ggagatgctg cgcgtgtaca cgcggcgcgc ggtcgaggcg atggtcgccg
     4921 ggggctaggc ggccgggtgt gcacgctgtt gttggcgtgg caggtcgacc cggcgcggcc
     4981 gctggtggtc gcggcgaacc gcgatgaatt ttacgcgcgc ccgagcgagc gtgccgcgtt
     5041 ctggcccgac gcgccgcagg tgctggccgg tcgagatctc gagggcggcg ggacctggct
     5101 cggtgtcacg cgcgacggtc gcttcgccgc gctgaccaac gtgcgcgagc ccggcgcgcc
     5161 gacccctccg caggcgccct cgcgcgggcg cctggtggcc gaattcctgc tcgctcgcgg
     5221 cgcgcccgac gagtatctgg ccggcctgcg ccccgcggac tatgccggct tcaacctcgt
     5281 cgtcggtgac gggcgctcgc tgtggtacct gtccaatcgg gcaggtccgg cgcgcgccct
     5341 cgggcccggc gtatatggcg tgtcgaacgc ggcgctcgat accccgtggc ccaagctgct
     5401 gcggggtcgc gagcgtctgg ccgcgctggt cgcgggtggg gcggatctgc cggcgctgct
     5461 ggagatgctc gaagatcggt caccgacggc cgacgccgag ctgccggaca cgggcgtcgg
     5521 gctggcgatc gagcgcatgc tgtccccgct gttcatcgcc agcccggcct acgggacctg
     5581 cagctcgacc gcgctggtcg tccaccggga cggggcgctg gagctccgcg agcgcacgca
     5641 aaaccccctc gcgcccgcgg gacccggcga ggtggcgtat gccctcgcgg cgccgccggc
     5701 cgcgtgaggg ctcgtctttt cgccgggttc gcggtactcc ttggcagcct cgtccggaga
     5761 ccgcatgtcg cgccgcacgc cacttcagag tctcgcccag atcttcgtcc tcgcgttcgt
     5821 gtccgccgcg gcactgcatg cgagcgtcgc ccacgccgcc gacggcgaca agcccaagcc
     5881 cgcctggcgc acggagaagc acatcctcga ggtcggcggc tacctcggcg cgctgttccc
     5941 cgccgccgac cacggcctct acgccgagaa catcaccaag acgcagcgcc cgatcaagac
     6001 cggcttcgat gtcggcatcc gcttcgcgta cctgccgctg cgcttcgtcg gcgtcgaggt
     6061 cgagggcggc atcgtcccga ccaagctcga cggcaacggc ggctacaaga ccacgctgtt
     6121 cgccgtgcgc ggccacctca tcctccagat gccgacccgc ctggccctgt tcgtcgtcgg
     6181 cggcggcggc atcctcggcg tcacctcgtc ggccaagggc ctcggcagca acgtcgacgg
     6241 cacgctgcac gtcggcggcg gcctcaagta ctatcttacg gagaaggtcg tcctgcgcat
     6301 cgacggccgc gacatcgcct ccccgaactt ctcccgcggc atggccggcg ggcaggactg
     6361 ggcgcacaac ggggaattca cgttcggggc gagcttcgtg atcggccgca agtcgaccaa
     6421 gatgctgccg cgcggcggct gaggcggtca tgcgggagcc cgcggcgccg ggcggtcgac
     6481 agcgagagca gccctggcgt gcacgctcga tgggcgagcg catgggtccg cccgcgtccg
     6541 cccgcgtccg cccgcgtccg cccgcgtcat ggctgctcga agcagatcag gttggcaccc
     6601 acgtcgcagt tgctcttgag cgcgtgggtc cagttgctgt cgctctccgt gctgacgcca
     6661 gtcgtcatca ccatcatcgg tggtgctcgc ctcgggcgcc ccggtgatga tgtgcgccgg
     6721 acacccgggc gcagtcacgg cgaggccggc gatgcgagcg acaagtttcg aggcacgcgc
     6781 tgcatgcacc gtgcattgca cgagcgcgct tccccgtcaa aacgcacccg tgggtgattc
     6841 atatgtaata tgaattatct attcgtcata ccgtgatctg ctggaaactc catcctgtac
     6901 cgcgggtcga gccgcgagcc gcgaccggtc gcggcgctcg aggtcgacgt gtgcctggac
     6961 ctcgacgtgg cgagggggtg tgcgctcgag gccgtgggtc gcaacacgac acgacggccg
     7021 cgacctgcgc cgaggccaag gaccggtgaa agccctggcg gacgcatcgg gtccgtggcg
     7081 acggctgcgc cggtctgccg agccggcgag gaggggggag ggcccgcgag catgagccca
     7141 tcgaggcgcc cgaaagcgcc gatcggaacg gccgggaccg agtcacgagc gcgcccgggg
     7201 ggccggccga ccgggcgagc tggatccaca ggccgggccg gattgtggat ccccagctgt
     7261 ggatggtggg gggctctcca ggatccaatg cgagaaagct gtggataact ccgggggctg
     7321 ccggggtcgc ggcagctttg aaggtaattg cgactacata aaaaaccggc gaaaactggc
     7381 ccctcgtggc agttttcgcg ttgatcgccg ccacgcattg aagctatggt gatcccacgg
     7441 cgccccccgc cgcagcttcg catgccggtc tcccaggccc accctagaga cgccgacgcc
     7501 cccgcggtcc gctccgcagg gcgaaagcgt ggcgacgtgc ccggtggcgt cctgaagcga
     7561 cgaaccctgc ggagcaatgc gaacggcgcg gtcgacctgt ccggacaggt gctcggcgga
     7621 cgctaccggc tcctgcgtcc gctcggccag ggcggcatgg cgatcatcta cgaggccgag
     7681 cacgtggcga tcggcaagcg ggtcgcgctc aaggtgctgg cgcaggcgcg gtgtagcgcg
     7741 acggtgcagc gcttcctgat ggaggcgcgc gcggccgcgg agctgcgcca cgagcacatc
     7801 atcgacgtca ccgacttcgg ccaggacacc ctcgccgacg gtgagccggt cgcgttcttg
     7861 gcgatggagt acctcgaggg cgaggacctc gcggccacgc tcgagcagga cggcccgctg
     7921 cgctggaccc gggtgctggc gatcggcaag cagatctgtc gcgcgctgat cgccgcgcac
     7981 gaccagggca tcgtccactg tgacatcaag ccggccaact gttatcgcat ccagcgcggc
     8041 gagacggcgg actacatcaa ggtgctcgac ttcggggtcg ccagcttcgc gtgcaagcgc
     8101 accggccggt gcacgccgga cccgatcccc gcgcccgaag gcggccgccc gcgctcgggc
     8161 ccacggatcg ggacccccgg ctacatggcg gccgagctgc tgcgcggcgg cagctacgac
     8221 caccgcgtcg acatctacgc gctcggcgtg ctgatgtacc gcctgctgac caacaagatg
     8281 ccgtacccgg ccgccaagct gtacgcggcc gcgagtgaca gcagccccgg acacgcgctc
     8341 gcgcgaccgg cgagcgcgac cggcgagtcg agcggcatga ccccggtggc gccgttcccg
     8401 atgcagcgcg ccatcccgac gctcgagatc tcaccggcct tcgaggcggt ggtgctccag
     8461 gcgatcgcgc acgaccccga gcagcgccag ccgaacgcgc gcgcgctcta cgaggcgctg
     8521 gtcgccgcgg agcaggcctc gatgcgcggc tcgctgctgc cgcgcgaccc gctgtgctgg
     8581 ggcgcgacgc gcgaggtgcc gcgcgccgac gactcgtcgt cgatgtcgca gacagaggtc
     8641 tcgcgcccgg ccttcgccgc gagcggcctg tcgaacgcgc agctgcgacg caccgccccg
     8701 gacctcggcc ccggcagctc gcacgcatcg caagcatcgc aagcatcgag cgctgcgagc
     8761 ggcgcgtggc gaccgacgac ccggcccgcg atgccgccgc tcgcggtgag ccccttcggt
     8821 ctgctcgtcc gcgcgacgct ggcgctcggt gtcacgaccg cggtcgtgcg cgcagcctgg
     8881 gcgctggtcg cctgagtcat cgcgtccact gcgtccactg cgtccactgc gtcatcgggt
     8941 catcgggtca tcgggtcatc gggtcatcgg gtca
//
LOCUS       DASWNJ010001200         2721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_587260, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001200 DASWNJ010000000
VERSION     DASWNJ010001200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587260"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="VGB85_20220"
     CDS             <1..728
                     /locus_tag="VGB85_20220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136426.1"
                     /translation="AQEPAAPEPASDPVEVADPFAASPSTVPAGAVRGGPPGVVPPAR
                     PPAPTAPASGPAAQVWETERPDTPAPGQLRRGLRVRSNYLHLAPGIIAADLRVADRYH
                     AWGVGFGRYFAGDSRFAASIGGFFEHAWLFREAPNVSPPLGATHLLRFGPEARLGASG
                     ERVFGYVLARIGIDVAIDRRGMTFSDRRRDVALMHTSVGVGLQVAPGPWHRLILGAEP
                     AVDLVFPAPLFLMRVRVFLGLRF"
     gene            complement(725..1876)
                     /locus_tag="VGB85_20225"
     CDS             complement(725..1876)
                     /locus_tag="VGB85_20225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136427.1"
                     /translation="MPRPSQIPLACLLVLCACGSPPAGPAKQDPKTEAKQTSTPEAQR
                     DPAPAARPEAAPADPTTPLANERHFAGKLRQLTNGGENAEAYFSADEQRLVFQSTRDG
                     GSCDQIYTMNVADLAVQRVSSGKGRTTCAYFLEKDARILYASTHAAADDCLPPPDRSR
                     GYVWKLYPEFDIYTARPDGSDIQPLVTGPGYDAEATVSPQGDRIVFTSTRDGDPELYV
                     IDADGKNLKRLTHAKGYDGGAFFSPDGKRIVYRANHPEGADELAKYDELVKDHLVRPT
                     RLELFVMNADGSEQKQITRNGKANFAPFFHPDGKRILFSSNQADPKGRDFDLFLINDD
                     GTGQEQVTFNPSFDGFPMFTRDGKSLVFASNRGAAKEGDTNVFLADWVD"
     gene            1929..2636
                     /locus_tag="VGB85_20230"
     CDS             1929..2636
                     /locus_tag="VGB85_20230"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY0136428.1"
                     /translation="MIDLVLEGPGKNALGTVLMREVQARLAAAGGAPVLLTGSGDAFS
                     AGLNLVEIAGLDEAGMRAYLELLDETIRALYLYPGPIVAFVNGHAIAGGCIFTLCCDH
                     RVCTPASSVKVGLNEVALGLRFPRGIFQLIRRRVAPQGLDEVVLGAQLHAPQDALRLG
                     LIDELGDLELARTRLAALARHPAGTYAGTKAELRDGVLVDDAAQRRAFLDAALPSWTA
                     PELKQRLLGFLKKPARG"
BASE COUNT          314 a          945 c         1017 g          445 t
ORIGIN      
        1 gcgcccagga gccggccgcg cccgagccgg cgtcggatcc cgtcgaggtc gccgacccgt
       61 tcgccgcgtc gccgtcgacc gtcccggccg gggccgttcg cggcgggcca cccggagtcg
      121 tgccgccggc acgaccgccc gcgccgacgg cccccgcgtc cgggcccgcg gcgcaggtgt
      181 gggagaccga gcggcccgat acgccggcgc ccgggcagct gcgccgcggt ctgagagttc
      241 gcagcaacta cctgcacctc gcgcccggca tcatcgcggc cgatctgcgg gtggcggacc
      301 gctaccacgc ctggggcgtc ggctttggcc gctacttcgc cggggactcg cggttcgcgg
      361 cctcgatcgg cgggttcttc gagcacgcat ggctgttccg cgaggcgccg aatgtctcgc
      421 cgccgctcgg cgccacccac ctgctccgct tcgggcccga ggcgcggctc ggcgccagcg
      481 gtgagcgggt gttcggctac gtgctcgcgc gcatcggcat cgacgtggcg atcgatcggc
      541 gcgggatgac cttctcggac cgtcgccgcg acgtcgcgct gatgcacacg tcggtcgggg
      601 tcggccttca ggtcgcgccc ggaccctggc accggctgat cctcggcgcc gagccggcgg
      661 tcgacctcgt gttcccggcc ccgctgttcc tgatgcgtgt gcgggtgttc ctcgggctgc
      721 gcttctagtc gacccagtcg gcgaggaaga cgttggtgtc gccctccttc gccgcgccgc
      781 ggttgctggc gaacaccagc gacttgccgt cgcgggtgaa catcgggaag ccgtcgaagc
      841 tcgggttgaa ggtgacctgc tcctggccgg tgccgtcgtc gttgatcagg aacagatcga
      901 agtcgcggcc cttcgggtcg gcctggttcg agctgaacag gatccgcttg ccgtccgggt
      961 ggaagaacgg ggcgaagttg gccttgccgt tgcgcgtgat ctgcttctgc tcgctgccgt
     1021 cggcgttcat cacgaacagc tcgaggcggg tcgggcggac caggtggtcc ttgaccagct
     1081 cgtcgtactt cgccagctcg tcggcgccct cggggtggtt ggcgcggtag acgatgcgct
     1141 tgccgtcggg cgagaagaag gcgccgccgt cgtagccctt cgcgtgggtc aggcgcttga
     1201 ggttcttgcc gtcggcgtcg atgacgtaga gctcggggtc gccgtcgcgg gtcgaggtga
     1261 acacgatgcg gtcgccctgc ggagacacgg tcgcctcggc gtcatagccg gggccggtga
     1321 ccagcggctg gatgtcgctg ccgtcgggcc tggcggtgta gatgtcgaac tcggggtaga
     1381 gcttccagac gtagccacgg ctgcgatccg ggggcggcag gcagtcgtcg gcggcggcgt
     1441 gcgtcgaggc gtagaggatc cgcgcgtcct tttcgaggaa atacgcgcag gtggtgcggc
     1501 ccttgccgct gctgacgcgc tgcaccgcga ggtcggcgac gttcatcgtg tagatctgat
     1561 cgcaggatcc gccgtcgcgc gtcgactgga acacgagccg ctgctcgtcg gcggagaagt
     1621 acgcctcggc gttctcgccc ccgttggtca gctggcgcag cttgccggcg aagtggcgct
     1681 cgttcgccag cggcgtggtc gggtcggccg gggcggcctc cggcctggcg gcgggcgccg
     1741 gatctcgctg cgcctcgggc gtgctcgtct gcttcgcctc ggtcttcgga tcttgcttcg
     1801 ccggcccggc gggcgggctg ccgcaggcgc acaggacgag caggcaggcg agggggatct
     1861 gcgacgggcg gggcatgcgg gcgcggatga taacaacgcg ggcggcggct ttgctatggt
     1921 cggcccgcat gatcgacctc gtcctcgaag gccccggaaa gaacgccctc ggcactgtat
     1981 tgatgcgcga ggtgcaggcc aggctcgcgg ccgcaggcgg ggccccggtg ctgctgacgg
     2041 gctcgggcga cgcgttctcg gccggcctca acctcgtcga gatcgccggc ctcgacgagg
     2101 ccggcatgcg cgcgtacctc gagctgctcg acgagacgat ccgggcgctc tacctctacc
     2161 cgggcccgat cgtcgccttc gtcaacggac acgcgatcgc cggtggttgc atcttcacgc
     2221 tgtgctgcga ccaccgcgtg tgcaccccgg cgagcagcgt caaggtcggg ctcaacgagg
     2281 tcgcgctcgg gctgcgattc ccgcgtggga tctttcagct gatccgccgg cgcgtcgcgc
     2341 cgcaggggct cgacgaggtc gtgctcggcg cgcagctgca tgccccgcag gacgcgctgc
     2401 ggctcgggtt gatcgatgag ctcggcgacc tcgagctcgc gcgcacgcgc ctcgccgcgc
     2461 tcgcccgcca ccccgcaggg acctacgccg gcaccaaggc cgagctgcga gacggcgtgc
     2521 tcgtcgacga cgccgcgcag cgccgcgcct tcctggacgc ggccctcccg agctggaccg
     2581 cgccggagct caagcagcgc ctgctcggct tcctcaagaa gccggcgcgc gggtagtcgt
     2641 ccgggccgct tgccaggcgg ggcggggagg cggacgatcg ccgcgatgcg tcgcgccgtc
     2701 ctgtgtctgc tgctcgcctg c
//
LOCUS       DASWNJ010001201         6148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_404377, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001201 DASWNJ010000000
VERSION     DASWNJ010001201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404377"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="VGB85_20235"
     CDS             <1..1021
                     /locus_tag="VGB85_20235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136429.1"
                     /translation="DAVLAAAAALQGSAARLRIELQAWSAGRALQPGPTLGLARLHES
                     IGHAATARALWQLAAFMVPQGPIAGQVAALPALPVPADVLANDREPALESREALRAVL
                     RRVAGETAGIRGRGEPPATASTPAEHEALAIAEAEVAPLRAALGLPLPLLLGRGGPDG
                     GVSVRNDRPPAIVVSAALAELPAAERRFRLALAAAMIAGGLAVVTDPQGASLPELLAA
                     LLHLADETCPARLAGAQTIVRAFAARGFTRERLPAGLREALAREVSRWQQDRSSLVRL
                     AHLLRRDSLRVATRLSGSLDGALRALGRDARLLPSGALDERGAAQVLASEDAQWLLRS
                     LGVFA"
     gene            1098..1931
                     /locus_tag="VGB85_20240"
     CDS             1098..1931
                     /locus_tag="VGB85_20240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136430.1"
                     /translation="MLILVGTAACSDEGGSASQTTKATTATTEDTGDDAGTSTPTSGA
                     ESGAESEVAEICVRYIDCIVATVPEALPAAQAGFGEGSMCWSGSAMMAMQCIDACKNA
                     LANSHEAFPTEQKCSVCVEDSECAVEEGEACLGGDCVLLDVCGNGVVEADEICDSGSG
                     CGDDCVSGPECSPLTQVGCGKSVCFYHAQGGLICVNPTGLPGNEAGEGCTDTFDCAAR
                     LACIAGEDLSGACASAACCVPYCDLAAPTTCAAGLTCRPLSESLDISEAGTDYLGICV
                     M"
     gene            2136..3923
                     /locus_tag="VGB85_20245"
     CDS             2136..3923
                     /locus_tag="VGB85_20245"
                     /inference="COORDINATES: protein motif:HMM:NF024209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEY0136431.1"
                     /translation="MTSRLIVLALATLTACSNDLALGDSDASSSGPSTDMSAGQTSAT
                     PTTGAPDSSSTGEPTSSTSTGGPDTSTSSDTSDTSTTTGDASSTTGGGDTSTGDEPGT
                     STTTGGMGEVPEGCEVVPIVDDVLLLQLQSKLNLPKQQPISGASALTVEALHLDPPKG
                     LTSLAGMQCFINLKSLIMHGGGVSDLGPLSTLPALELLEIESNTISDLGPLVGAPALR
                     KLYVSGNQIVDLAPVVAMPELEELEIPHNPITSFAALAGHPSLRRLDGSYTKTTNLDG
                     LGALPKLDTLVLSHAGVTDIALLASVTGLVSLDLSQNAIADISALAGMSGLTTLKINN
                     NKVVDIAALAGKPALVDLDLSINQIGDIAALAGDTALVDVNLGGNAVTDLSVLASMPA
                     LAIVRVAGNMVTDLSPLAGHAALYELSAQHNQIADVTGLVDLPVLQRLFLSGNQISDP
                     APLGGLSSLTYLYLDENPLAAGIAGLAPLSKLESLEVSSTGITELIAISPATLLALFA
                     ADNTIVDLSPLAGHSELGYLGLKNNQITTISPIAMAPYWDGTCATLEITGNPLDVQTT
                     SVFLPSLCDLQNSVFWDGGGCGGCEPAPP"
     gene            complement(3930..>6148)
                     /locus_tag="VGB85_20250"
     CDS             complement(3930..>6148)
                     /locus_tag="VGB85_20250"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY0136432.1"
                     /translation="CAPVPSQPHYRQAVEADLQRAMQALVRPGKGYVGCKEVLETLVA
                     PGARLAELASLPLGTLGHRLMALRYFIANGATWLRFFAWEPALSLVASEGPDKIRALA
                     ARIESARSGLPAEEELARELDGQTLLALAGTLETASELGDGALRLLCAIAGGPALATR
                     LRERLRAEAGVLHREAPRAADLIYLASHDLSRFPATTVGEAALHYVIVADKGEMGVRA
                     VREAVALGKVPVVLHSAQDDANALQVRLADRHGGFAIPLTGSFRESYANPVQMARKIV
                     EAYGTRFGSSAEAELARSALYPGYGPLAESTVAIEFFRRSGIVFVGPMQDVVERAGDK
                     RKFRLLAQSIDEHAVVPGIVIDVVDPAAIIAAIRAGHAQGRFQFPGRLKAANGGGGRG
                     QMVVPSEAGIEAAVTKVLGEITANGWDHGVMFEQNIAETVHLEVQVLRDRYGNTRHFG
                     MRDCSEQRASQKIQEEAPPAIMRAYPGLTERICAVAVRIADAVGYTGACTVELMFKNG
                     HFFLLEMNTRIQVEHPVSEEAHRIRTPAGLEPLNLVALQLAIANGAPLSFAQEDIVNT
                     HVAREFRINAESWRADVKDSRDGKLGLFVPNAGSFDTITVPDATQVRAALEGRGVTGI
                     VDLTVRFDCGFEVKDKLVNKDPTFGKLIVAVAADEAHAEQRYELLRQASLEVLGSMSI
                     EGQGLMPDGSIIRGARFQTNLTDHIRVLKSECMIEHAQRVVPGRHVNWVVESLRRA"
BASE COUNT          869 a         2095 c         2269 g          915 t
ORIGIN      
        1 cgacgcggtg ctggcggccg cggcggcgct gcagggctcg gcggcgcggc tgcggatcga
       61 gctgcaggcg tggtcggccg ggcgcgcgct gcagccgggg ccgacgctcg ggctggcgcg
      121 cctgcacgag tcgatcgggc acgccgcgac ggcgcgcgcg ctgtggcagc tggcggcgtt
      181 catggtgccg caggggccga tcgcggggca ggtggccgcg ttgccggcgt tgccggtgcc
      241 ggccgacgtg ctggccaatg accgcgagcc cgcgctcgag tcgcgcgagg cgctgcgggc
      301 ggtgctgcgg cgggtggccg gcgagacggc ggggatccgc gggcgcggcg agccgccggc
      361 gacggcgagc acgcccgcgg agcacgaggc gctggcgatc gcggaggccg aggtggcgcc
      421 gctgcgggcg gcgctcgggc tgccgctgcc gctgttgctc gggcgcggcg ggccggacgg
      481 aggggtcagc gtgcgcaacg atcgaccacc ggcgatcgtc gtcagcgcgg cgctggcgga
      541 gctgccggcg gccgagcgca ggttccggct cgcgctcgcg gcggcgatga tcgcgggcgg
      601 gctggcggtc gtcaccgacc cgcagggagc gtcgctgccg gagctgctgg cggcgttgct
      661 gcacctggcc gatgagacat gtcccgcgcg cctcgccggg gcgcagacga tcgtgcgcgc
      721 gttcgcggcg cggggcttca cccgcgagcg cctgccggcc gggctgcgtg aggcgctcgc
      781 gcgcgaggtg tcgcgctggc agcaggaccg cagcagcctg gtccgactcg cgcacctgct
      841 gcgccgcgac agcctgcgcg tggcgacgcg gctgtcgggc agcctcgacg gcgcgctgcg
      901 agccctcggt cgcgacgcgc ggctgctgcc gtccggcgcg ctcgacgagc gcggcgcggc
      961 gcaggtgctc gccagcgagg acgcgcaatg gttgctgcgc agcctcgggg tgttcgcgtg
     1021 agcgagcgcc gtgcttgtcc gcggatgtcg gcgtcggcat actcggaggg atgagagatc
     1081 gtctgcggtt tacgtgcttg cttatcctcg tcggcacggc ggcctgttcg gacgaagggg
     1141 gctcggcgtc acagacgacg aaagcgacga ccgcgacgac ggaggacacg ggcgatgatg
     1201 ccggcacctc cacgccgacc agcggcgccg agtcgggcgc cgagagcgag gtcgccgaga
     1261 tctgcgtgcg ttacatcgat tgcatcgtgg cgacggtccc ggaggcgctg cccgcggcgc
     1321 aggcgggctt cggcgagggc agcatgtgct ggagcggcag cgcgatgatg gcgatgcagt
     1381 gcatcgacgc ctgcaagaac gcgctcgcca acagccacga ggcgttcccc accgagcaaa
     1441 agtgcagcgt gtgcgtggag gacagcgagt gcgcggtcga ggagggcgag gcctgtctcg
     1501 gcggagattg cgtgctgctc gacgtgtgcg gcaacggggt cgtcgaggcc gacgagatct
     1561 gcgactcggg gtccggatgc ggcgacgatt gcgtgtccgg gccggagtgc agcccgctca
     1621 cccaggtcgg atgtgggaag tccgtttgct tctaccatgc gcagggcggc ctcatatgcg
     1681 tcaacccgac cggcctgccg ggtaacgagg caggcgaggg ctgcaccgac accttcgact
     1741 gtgccgcccg actcgcatgt atcgccgggg aggacctctc cggggcatgc gcgagcgctg
     1801 cttgctgcgt accgtattgc gacctcgcgg cgccgacgac ctgcgcggcg gggctgacgt
     1861 gccggcccct ctccgagtct ctggatatca gcgaagccgg gaccgactat ctcgggatct
     1921 gtgtgatgtg acggccgggc gcggcggtgc ggctgctgcc gtccggtatg ctcgacgagc
     1981 gcggcgcggc acaggtgctc gccagcgagg acgcgcaatg gtcgctgcgc agcctcgggt
     2041 gttcggctgg gggcgggcgt tcccacaact ggaagctcgt gcctgtgcgg tttccagagt
     2101 ggaccgcggg tacgacctct gcgagcgtgg actccatgac ttcacgtctc attgtgctcg
     2161 ccctggcgac gctgaccgcc tgctcgaacg acctcgcgct cggcgacagc gacgcgagca
     2221 gttcgggacc atctacggac atgtcggcgg gccagaccag cgcgacaccg acgactggtg
     2281 cgccggactc gtcgagcacg ggtgagccga cgtcgtcgac gagcacgggt ggaccggata
     2341 cgtcgacgag cagtgacacg agcgacacct cgacgaccac aggcgacgcc tcgtcgacca
     2401 ccggcggggg cgacacctcg acgggcgacg aaccggggac ctcgacgacg accggtggga
     2461 tgggcgaagt ccccgagggc tgtgaggtgg tcccgatcgt cgacgatgtg ctgctgttgc
     2521 agcttcagag caagctcaat ctgcccaagc agcagccgat ctccggggcg agcgcgctga
     2581 cggtcgaggc gctgcatctc gacccgccga agggcttgac gagcctcgcg ggcatgcagt
     2641 gcttcatcaa cctcaagagc ttgatcatgc acggcggcgg ggtctccgac ctcgggccgc
     2701 tgtcgacgct gcccgcgctg gagctgctgg agatcgagag caacacgatc tccgacctcg
     2761 ggccgctggt cggcgcgccg gcgctgcgca agctgtacgt cagcggcaac cagatcgtcg
     2821 acctcgcgcc ggtcgtggcg atgccggagc tcgaggagct ggagatcccg cataacccga
     2881 tcacctcctt cgcggcgctc gccggtcacc cgtccctgcg gcggctcgac ggctcgtata
     2941 ccaagaccac caacctcgac gggctcggcg cgctgccgaa gctcgacacg ctggtgctca
     3001 gccacgcggg cgtgaccgac atcgccctgc tggcgagcgt gaccggcctg gtgtcgctcg
     3061 acctgtcgca aaatgccatc gccgacatct cggcgctcgc gggcatgtcc gggctcacca
     3121 cgctcaagat caacaacaac aaggtcgtcg acatcgcggc gctcgccggc aagccagcgc
     3181 tcgtcgacct cgacctcagc atcaaccaga tcggcgatat cgcggcgctc gccggcgaca
     3241 ccgcgctcgt cgacgtcaac ctcggtggca acgcggtcac cgacctctcc gtgctggcga
     3301 gcatgccggc gctcgccatc gtgagggtcg cggggaacat ggtcaccgac ctgtcgccgc
     3361 tcgccgggca cgccgcgctc tacgagctga gcgcgcagca caatcagatc gcggacgtca
     3421 cggggctcgt cgacctgccc gtgctgcagc ggctgttcct gtcgggcaat cagatctccg
     3481 accccgcgcc gctcggcggt ctgtcgagcc tcacctacct ctacctcgac gagaacccgc
     3541 tggccgccgg gatcgccggg ctcgcgccgc tgtccaagct cgagtcgctc gaggtgtcga
     3601 gcaccggcat caccgagctg atcgcgatct caccggcgac gctgctggcg ttgttcgcgg
     3661 ccgacaacac gatcgtcgac ctgtcgccgc tcgccgggca cagcgagctc ggttacctcg
     3721 gcctcaagaa caaccagatc acgacgatct cgccgatcgc catggcaccg tactgggacg
     3781 ggacctgcgc gacgctcgag atcaccggga acccgctcga cgttcagacg acgagcgtgt
     3841 tcctgccgag cctgtgtgat ctgcaaaact ccgtgttctg ggacggcggc ggctgtgggg
     3901 gctgcgagcc ggcgccgccg tgagccgctt taggccctgc gcagcgactc gacgacccag
     3961 ttgacgtgac gaccggggac gacgcgctgc gcgtgctcga tcatgcactc ggacttgagg
     4021 acccgtatgt ggtcggtcag gttggtctgg aagcgtgcgc cgcggatgat gctgccgtcg
     4081 ggcatcaagc cctggccctc gatgctcatg ctgccgagga cctcgagcga ggcctgccgc
     4141 aacagctcgt agcgctgctc ggcgtgcgcc tcgtcggccg cgaccgccac gatcaacttc
     4201 ccgaaggtcg ggtccttgtt caccagcttg tccttgacct cgaacccgca gtcgaagcga
     4261 accgtcaggt cgacgatgcc ggtcacgccg cgtccttcga gggccgcgcg cacctgcgtc
     4321 gcgtccggca ccgtgatcgt gtcgaacgag cccgcgttgg gcacgaacag gccgagcttg
     4381 ccgtcgcgcg agtccttgac gtccgcgcgc catgactccg cgttgatccg gaactcgcgc
     4441 gcgacgtggg tgttgacgat gtcctcctgc gcgaacgaga gcggagcacc gttggcgatc
     4501 gccagctgca gcgcgacgag gttgagcggc tcgagaccgg cgggcgtgcg gatccggtgc
     4561 gcctcctcgc tgaccgggtg ctcgacctgg atccgcgtgt tcatctcgag caggaaaaag
     4621 tgcccatttt tgaacatcag ctcgacggtg cacgcgcccg tgtagccgac ggcgtcggcg
     4681 atgcgcacgg cgacggcgca gatgcgctcg gtgaggccgg ggtaggcgcg catgatcgcg
     4741 ggcggggcct cctcctggat cttctggctg gcgcgctgct cgctgcagtc gcgcatcccg
     4801 aagtggcgcg tgttgccgta gcggtcgcgc agcacctgca cctcgaggtg caccgtctcg
     4861 gcgatgttct gctcgaacat cacgccgtga tcccagccgt tggccgtgat ctcgccgagc
     4921 accttggtga ccgcggcctc gatgcccgcc tcggacggga cgaccatctg tccgcgccca
     4981 ccaccgccgt tggcggcctt gagtcgcccg gggaattgaa agcgtccctg cgcgtggccg
     5041 gcacgaatgg cggcgatgat ggcggccggg tcgacgacgt cgatgacgat gcccggcacc
     5101 acggcgtgct cgtcgatcga ctgggccagc aggcggaact tgcgtttgtc gccggcacgc
     5161 tcgacgacgt cctgcatcgg cccgacgaag acgatgccgc tgcgccggaa gaattcgatc
     5221 gccacggtgc tctcggcgag cggcccgtac ccgggataca gcgccgagcg ggcgagctcg
     5281 gcctcggcgc tggagccgaa gcgcgtgccg taggcctcga cgatcttgcg cgccatctgt
     5341 acggggttgg cgtagctctc gcggaagctg cctgtcagcg ggatggcgaa gccgccgtgc
     5401 ctgtcggcga ggcgcacctg cagcgcgttg gcgtcgtcct gcgcgctgtg caggaccacc
     5461 gggaccttgc cgagcgcgac ggcctcacgc acggcccgca cgcccatctc gcccttgtcg
     5521 gcgacgatga cgtagtgcag cgccgcctcg ccgacggtgg tcgccgggaa gcgcgagagg
     5581 tcgtggctgg cgaggtagat caggtcggcg gcgcgcggcg cctcgcggtg cagcacgccg
     5641 gcctcggcgc gcaggcgctc gcgcaggcgg gtggcgagcg cgggcccacc ggcgatcgca
     5701 cacaacagtc gcagcgcgcc gtctccgagc tcgctcgcgg tctcgagggt gccggcgagc
     5761 gcgagcaggg tctggccgtc gagctcacgg gcgagctcct cctcggcagg caggccgctc
     5821 cgggcgctct cgatgcgcgc cgcgagtgcg cggatcttgt cggggccctc gctggcgacg
     5881 agcgacagcg ccggctccca ggcgaagaag cgcagccagg tggcgccgtt ggcgatgaag
     5941 tagcgcagcg ccatgagccg gtggccgagc gtaccgagcg gtagcgaggc gagctcggcg
     6001 agccgcgctc ccggtgcgac gagcgtctcc aggacctcct tgcagccgac gtagcccttg
     6061 cccggcctga cgagggcctg catggcgcgc tggaggtcgg cttcgacggc ttggcgataa
     6121 tggggctgac tcggcacggg cgcgcaat
//
LOCUS       DASWNJ010001202         2711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_333852, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001202 DASWNJ010000000
VERSION     DASWNJ010001202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333852"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VGB85_20255"
     CDS             <1..865
                     /locus_tag="VGB85_20255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136433.1"
                     /translation="TDPAKPGSLVVYDVAQKKKVREIAPAGTVDDLILPLALPRELAA
                     SLKEYQAPPPPATEAELKVIAELDEPSKTPEELRAEADAIKRAQESVDTGMSEAVDTS
                     GVTVDLNKDFKTYCRTGPRKPRVNKDDPKDLRPACKMGPKFGYFQTRGWVALGLTAGA
                     LVTTGLFYTLGMVARKPYKDANAKLEASGFNPLDPTMSTEYTALAADTAAKGNTMRKR
                     FVGGDVALLASVLLGAVLGIIIYQDRTDAKEFIRTEKSLKAVSRFKPKNFRGGPAFDR
                     TMQGVTFGFDF"
     gene            complement(1058..1837)
                     /locus_tag="VGB85_20260"
     CDS             complement(1058..1837)
                     /locus_tag="VGB85_20260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136434.1"
                     /translation="MIGRTRRIRPSLPLASGLALGLALVAACDDSGETRKDGAPTTAG
                     ATAGNVPGDGKIAAPPALTKYKSPAEAIGAYNKCYSDCFSSGGNSTNRETCKLDCDTL
                     VETGMDTLPDEASKATYKTTWQTLRTCINGCWGDRKLNETNRATCLLTCSDNAEVSAI
                     PVPNGPGAQPGAPAGTGTPPATGTPPTKTGTGTATTPGAATTPGAATGTTPGRTAPAT
                     GTPPAAGTPGKTAPTTGTPPATGTSPARPTSPGPTPVTGNR"
     gene            1968..>2711
                     /locus_tag="VGB85_20265"
     CDS             1968..>2711
                     /locus_tag="VGB85_20265"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0136435.1"
                     /translation="MTWALSAQGARLIVRDGDAAPVAVNLERRPLMLGRTADNDIVVN
                     AQFVAAHHARIEPVGADYRIVDLGGACGIMFGGQRVGTHNLRDGDVIRIGDPLTGSFV
                     TLLYQRNATSTTVPAAASTVASIPLDQRITTIGREGSALTLASPLVSRAHAEVGRLPG
                     GGHILRDLGSTNGTFVGGQRVTVPRLLQPGQLIQIGPFKLAYDGNSLLPFDQRGAMTL
                     EARDLVRTVAAGRVILNKVSLIIQPREFVA"
BASE COUNT          383 a          930 c         1015 g          383 t
ORIGIN      
        1 gaccgacccg gccaagcccg gctcgctcgt cgtctacgac gtcgcgcaga agaagaaggt
       61 ccgcgagatc gccccggccg gcacggtcga cgacctgatc ctgccgctgg cgctgccgcg
      121 cgagctggcg gcgagcctca aggagtatca ggccccgccg ccgccggcga ccgaggccga
      181 gctcaaggtg atcgccgagc tcgacgagcc gtcgaagacg cccgaggagc tgcgcgccga
      241 ggccgacgcg atcaagcggg cgcaggagtc ggtcgacacc ggcatgagcg aggcggtcga
      301 caccagcggc gtcaccgtcg acctcaacaa ggacttcaag acctactgtc gcacgggccc
      361 ccgcaagccg cgcgtcaaca aggacgaccc caaggacctg cgccccgcgt gcaagatggg
      421 gccgaagttc ggctacttcc agacccgcgg ctgggtcgcg ctcggcctga ccgccggcgc
      481 gctggtcacc accggcctgt tctacacgct cggcatggtc gcccgtaagc cgtacaagga
      541 cgccaacgcc aagctcgagg cgtcgggctt caacccgctc gacccgacga tgagcaccga
      601 gtacacggcg ctcgcggccg acactgcggc caagggcaac acgatgcgca agcgcttcgt
      661 cggcggcgac gtggccctgc tcgcgtccgt gctgctcggc gcggtgctcg gcatcatcat
      721 ctatcaggac cgcaccgacg cgaaggagtt catccgcacg gagaagagcc tcaaggcggt
      781 gtcgcggttc aagcccaaga acttccgcgg cggcccggcc ttcgaccgga cgatgcaggg
      841 cgtgaccttc ggcttcgact tctgaagatc tctcgagata agctcgagac aagaaagagg
      901 caccgtcgct ggcgacggtg cctcttctcg ttcgtggttt cggaggtcgg ggagatccgt
      961 gcgcgcgagg ccggcccgcg atcgggccgg tctcggaaca gtgcgcgcga gtgcgcgagg
     1021 gatcggccct gcgatcgtgc gcagggcacg cgcgcgatca gcggttgcca gtgaccggcg
     1081 tcggacccgg cgaggtcggc cgcgcgggcg aggtgccggt ggccggcggg gtgccggtcg
     1141 tcggcgcggt cttgccaggc gtgccggcgg cgggcggcgt gccggtggcc ggcgcggtcc
     1201 ggccgggcgt cgtgccggtg gcagcgccgg gcgtggtggc agcgccgggc gtggtggcgg
     1261 tgccggtgcc ggtcttcgtc gggggcgtgc cggtggccgg cggggtgccc gtgccggcgg
     1321 gggcgccggg ctgggcgccg gggccgttgg ggacggggat cgccgagacc tcggcgttgt
     1381 ccgagcaggt cagcaggcag gtcgcgcggt tggtctcgtt gagcttcctg tcgccccagc
     1441 agccgttgat gcacgtgcgc agggtctgcc aggtggtctt gtaggtcgcc ttggaggcct
     1501 cgtccgggag ggtgtccatg ccggtctcga ccagggtgtc acagtcgagc ttgcaggtct
     1561 cccggttggt cgagttgccg ccggacgaga agcagtcgct gtaacacttg ttgtaggcgc
     1621 cgatcgcctc ggccgggctc ttgtacttcg tcagggccgg gggggcggcg atcttgccgt
     1681 caccgggcac gttgcccgcg gtggcgccgg cggtggtcgg cgcgccgtcc ttgcgggtct
     1741 cgccggagtc gtcgcacgcg gcgaccagcg ccagaccaag cgcgaggccc gaagcgaggg
     1801 ggagggaggg tcggatcctg cgggttctgc cgatcatgcc ggcaaggata gggcacatgg
     1861 gcgaccccgg cgaagatttg acaggccggg ccggtggcgc gaaggtttgt ccgcccttgc
     1921 cggccgatcc cgcagcccgc ccgaccgtcc ccggcgagtc aggcgatatg acctgggcgc
     1981 tcagcgccca gggagcgcgg ctcatcgtcc gcgatgggga cgcggcaccg gtcgcggtga
     2041 acctcgagcg gcgtccgctg atgctcgggc gcacggcgga caacgacatc gtcgtcaacg
     2101 cccagttcgt cgcggcccac cacgcgcgca tcgagccggt cggcgccgac tatcgcatcg
     2161 tcgacctcgg tggcgcgtgc gggatcatgt tcgggggcca gcgcgtcggc acccacaacc
     2221 tgcgcgacgg cgatgtcatc cgcatcggcg acccgctgac cggcagcttc gtcacgctgc
     2281 tgtaccagcg caacgcgacg agcacgacgg tcccggcggc ggcgagcacg gtcgccagca
     2341 tcccgctcga ccagcggatc acgacgatcg gtcgcgaggg cagcgcgctg accctggcga
     2401 gcccgctggt gtcgcgggcc cacgccgagg tcggccggct gccgggcggc gggcacatcc
     2461 tgcgcgacct cggcagcacc aacggcacct tcgtcggagg ccagcgggtc acggtgccgc
     2521 gcctgctgca gcccggccag ctgatccaga tcgggccgtt caagctcgcg tacgacggca
     2581 actcgctgct gcccttcgac cagcgcggcg cgatgaccct cgaggcgcgc gacctggtgc
     2641 gcacggtcgc ggccggccgc gtgatcctca acaaggtctc gctgatcatt caaccgcgcg
     2701 agttcgtggc g
//
LOCUS       DASWNJ010001203         4057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_161073, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001203 DASWNJ010000000
VERSION     DASWNJ010001203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161073"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VGB85_20270"
     CDS             <1..622
                     /locus_tag="VGB85_20270"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF002288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEY0136436.1"
                     /translation="VAAEAVLGLGLAPTQLVDLAREGELAACGAAHPDNVAPAILGGI
                     VLIPCLEPLRVVNLPSPKNLWLAVYTPGCSVATADARRVLPKQVALASAVQQAARLGL
                     LVHALHEGDLELLGEAIADDIVEPVRAHLIPGFLDAKAACQEAGALACSISGSGPTTF
                     ALAASSSRAEALLQILDECFTLIGVPGKGHVDQVGPGARVLSTPLH"
     gene            complement(1036..2772)
                     /locus_tag="VGB85_20275"
     CDS             complement(1036..2772)
                     /locus_tag="VGB85_20275"
                     /inference="COORDINATES: protein motif:HMM:NF041770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CFI-box-CTERM domain-containing protein"
                     /protein_id="HEY0136437.1"
                     /translation="MSRVPGQLATRLVGALALLCAVDARAAEDLLVEVHFKPVPGAQI
                     AIWLEDDAGKWVQDILVTQAVGKLGIGNRPGLWNFLSSWRAPYGPRPSVLPIWAHRRG
                     KTYPKLVFYDNNPADKESLGYHESTSSAETYYCRPLTAAEHDVIAVDTMTCPSPAVFQ
                     TDKGVFDPVATSVYPPRNDLLTFEDKQDSPDAMKLAALNDLDAVTAATPPGDAPEHAT
                     AMIPGAKAQGPLTAWIEISLEHDENPSHDYDRADDHYIDPRLGAYGVEYLGQPSVVYK
                     LPFDPRIEGFRGTSTWAGYGDWNGATGTIHPPDATISTTKGSGADRLQQYTLNNETFR
                     FGVYSYGSGVVPPDPDTGDTLDADTTTDPDTGDTGDTGGWGHCQPRPLPPVTGLMLEG
                     LSFDRVRVTYTLPPQTDPDFMLSRMRVHLLNGETPLTQDRLPSAQLPAFQVIEAATPG
                     ASGVLEVDQLWGNFTYQVGVTYQDKCGNTSALSSAEITTEAQKFQQVEGFCFLATAAY
                     GGAWVSQVQALRWFRDAYLKTSPIGRDLVRFYYTYSPPLARIVAHQPVLRGTVRVVLQ
                     PIADLARLGARG"
     gene            complement(2791..3063)
                     /locus_tag="VGB85_20280"
     CDS             complement(2791..3063)
                     /locus_tag="VGB85_20280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136438.1"
                     /translation="MTASLRAPSPAVLAVLTALLGLTLGVACRISNEDHCVHKALDSD
                     AWCAEQVPGKPFCSPCEAVDHGCVAARPDDEDCPAYAPDSSSGESG"
     gene            complement(3100..3678)
                     /locus_tag="VGB85_20285"
     CDS             complement(3100..3678)
                     /locus_tag="VGB85_20285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136439.1"
                     /translation="MAASSIAATLIVALVAPASPPTRAATSSPAPASTAPASTPAPAS
                     TPASPRATTTPPASPPAPVPGDMSARPAARAVEGPAPAPATPVTPRGICHGSYPCKRL
                     VVFSTVTGALGLATIVTGAVIAARPVTVDPDDPTMAIAYRPAGTAVLTIGIGVLATSV
                     LMALAAARASRQAARRGPLARRAPRVAPLLAH"
     gene            complement(3684..>4057)
                     /locus_tag="VGB85_20290"
     CDS             complement(3684..>4057)
                     /locus_tag="VGB85_20290"
                     /inference="COORDINATES: protein motif:HMM:NF041770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CFI-box-CTERM domain-containing protein"
                     /protein_id="HEY0136440.1"
                     /translation="DVEQLWGNYTYQIGVTYQDKCGNTSALVSTAITTEAQKFQQVEG
                     FCFLATAAYGAAWVGQVQALRWFRDAYLKTSPVGRDLVRFYYTYSPPLARIVHHQPVL
                     RGMVRVVVQPIADMARLSTRG"
BASE COUNT          546 a         1295 c         1626 g          590 t
ORIGIN      
        1 ggtcgccgcg gaggcggtgc tcgggctcgg gctcgcgccg acgcagctgg tcgacctggc
       61 gcgtgagggc gagctggcgg cctgcggggc ggcgcacccc gacaacgtgg cgccggcgat
      121 cctcggcggg atcgtgctga tcccgtgcct cgaaccactg cgcgtcgtca atcttccgag
      181 cccgaagaac ctgtggctcg cggtgtatac gccgggctgc tcggtcgcca cggcggacgc
      241 ccggcgcgtg ctgccgaagc aggtggcgct ggcgtcggcg gtgcagcagg cggcgcgcct
      301 cgggctgctg gtccacgcgc tgcacgaggg ggacctcgag ctgctcggcg aggcgatcgc
      361 ggacgacatc gtcgagccgg tgcgcgcgca cctgatcccc gggttcctcg acgccaaggc
      421 ggcgtgtcag gaggccgggg cgctggcgtg cagcatcagc ggctcggggc cgacgacctt
      481 cgcgctcgcg gcctcgtcgt cgcgcgccga ggcgctgctg cagatcctcg acgagtgctt
      541 cacgctcatc ggggtgcccg gaaagggaca cgtcgatcag gtcgggcccg gggcgcgcgt
      601 gctgtcgacg ccgctgcact gagggacgca tccgttggcc agtgtggtag accccgaatg
      661 accgctccac tccaaacccg cctgctgctc tggacgctcg cggcgatggt cgctgccggc
      721 tgctcacctg ccgccgagtc cgaggtggct ccgccgaccg ggccagtggt cgcggcgagg
      781 cccgcacccg ccgaaccagg agcgccagcg gagtgcatgg ccgacgcgga ttgcctgcgg
      841 acccactgct gcggggtcgg tgccggctgt gctccggcgc cggagggtca ggcgtgcgcc
      901 gatgcggtct gctccatcag cgtgtggagc ctctgccgtt gcgaggcgag tcgctgcacg
      961 ggcctcttct gggatccgac ctccgacaag ccgaggcccg cagaattcgc cgattggtaa
     1021 cgagcaggcc tcgcatcagc cgcgagcgcc gaggcgggcc aggtcggcga tcggctggag
     1081 aacgacgcgg accgtgccgc gcaggaccgg ctggtgggcg acgatgcgcg cgagcggcgg
     1141 gctgtatgtg taataaaaac ggaccaggtc gcggccgatc ggcgacgttt ttaaatatgc
     1201 atcgcggaac catcgcaggg cctggacctg gctgacccag gcgccgccgt aagccgcggt
     1261 ggcgaggaag cagaagccct cgacctgctg gaacttctgg gcctcggtcg tgatctcggc
     1321 ggagctgagg gcgctggtgt tgccgcactt gtcctgatag gtgacgccga cctgatatgt
     1381 aaaattgccc cacagctgat cgacctcgag cacgccgctg gccccgggcg tcgcggcctc
     1441 gatgacctgg aaggccggca gctgggcgga gggcaggcgg tcctgcgtca gcggcgtctc
     1501 accgttgagc aggtgcacgc gcatccgcga gagcatgaag tcggggtcgg tctgcggcgg
     1561 gagcgtgtaa gtgacgcgga ccctgtcgaa gctgagaccc tcgagcatca ggccggtgac
     1621 cggcgggagt gggcgcggct ggcagtgacc ccagccgccg gtgtcgccgg tgtcgccggt
     1681 gtccgggtcg gtggtggtgt cggcgtcgag cgtgtcgccg gtgtcggggt cgggcgggac
     1741 gacgccggag ccgtagctgt agacgccgaa gcggaaggtc tcgttgttga gggtgtactg
     1801 ctggaggcga tcggcgccgc tgcccttggt ggtggagatc gtggcgtcgg gggggtggat
     1861 cgtgccggtg gcgccgttcc agtcgccgta gccggcccag gtcgaggtgc cgcggaagcc
     1921 ctcgatgcgc gggtcaaagg gcagcttgta gacgaccgag ggctggccga gatactcgac
     1981 gccgtaggcg ccgaggcgcg ggtcgatgta atgatcgtcg gcgcggtcat agtcgtggga
     2041 ggggttctcg tcgtgctcga gcgagatctc gatccaggcg gtcagcgggc cctgggcctt
     2101 cgcgccgggg atcatcgcgg tcgcgtgctc gggcgcgtcg ccgggcgggg tcgcggcggt
     2161 cacggcgtcg aggtcgttga gcgcggcgag cttcatcgcg tccggtgagt cctgcttgtc
     2221 ctcgaaggtc agcaggtcgt tgcgcggcgg atagacgctg gtggcgaccg gatcgaagac
     2281 gcccttgtcg gtctggaaca ccgcgggcga cggacaggtc atcgtgtcga ccgcgatgac
     2341 gtcgtgctcg gcggcggtca gcgggcgaca gtagtaggtc tccgcggagc tggtgctctc
     2401 gtggtagccg agcgactcct tgtcggccgg gttgttgtcg tagaagacga gcttcggata
     2461 ggtcttgccg cgccggtggg cccagatcgg cagcaccgag ggccgcgggc cgtaaggcgc
     2521 gcgccagctc gacaggaagt tccacagacc gggacggttg ccgatgccga gcttgccgac
     2581 agcttgcgtg acgaggatgt cctgcaccca cttgccggcg tcgtcctcga gccagatcgc
     2641 gatctgggcg ccgggcaccg gcttgaagtg gacctcgacc agcaggtcct cggcggcgcg
     2701 cgcatccacg gcgcacagga gcgcgagcgc cccgacgagg cgcgtggcga gctggcccgg
     2761 gacgcgtgac atccgggcag tatacacgcg tcagccggac tcgccgctgc tgctgtcggg
     2821 cgcgtaggcg gggcagtcct cgtcgtccgg gcgcgcggcg acgcagccgt gatcgaccgc
     2881 ctcgcagggc gagcagaacg gcttgccggg gacctgctcg gcgcaccagg cgtcgctgtc
     2941 gagcgccttg tggacacagt gatcctcgtt ggagatccgg caggcgacgc cgagcgtcag
     3001 gccgagcaga gccgtgagga cggccaggac cgcggggctc ggcgcgcgca gggaggccgt
     3061 cacgcggcga gcatcgcatg caacgcggcg cgcggccagt cagtgtgcga gcagcggcgc
     3121 gacgcggggg gcccggcgcg cgagggggcc acggcgggcg gcttgccggc tcgcccgcgc
     3181 ggcggccagg gccatcaaca cgctggtcgc cagcacgccg atgccgatcg tcagcaccgc
     3241 cgtgccggcc gggcggtagg cgatcgccat cgtcgggtcg tcggggtcga cggtgaccgg
     3301 cctggccgcg atcacggccc cggtgacgat cgtcgccagg ccgagcgcgc ccgtgacggt
     3361 gctgaacacg accagacgct tgcacgggta cgagccgtgg cagatcccac ggggcgtcac
     3421 gggggtcgcg ggcgccggcg ccggtccctc gaccgcgcgc gcggctggcc gcgcggacat
     3481 gtcccccggg acaggtgccg gcggggacgc ggggggcgtc gtggtggcgc gcggggacgc
     3541 cggggtgctc gcgggagccg gagtgctcgc gggagcggtg ctcgcgggag ccggggagct
     3601 cgtcgcggcc cgcgtgggcg ggctcgcggg ggcgacgagg gcgacgatca gggtcgcggc
     3661 gatcgacgac gcggccacgg tcgtcagcct cgcgtgctca ggcgggccat gtcggcgatc
     3721 ggctggacga cgacgcggac catgccgcgc aggaccggct ggtggtggac gatgcgggcg
     3781 agcggcgggc tgtacgtgta gtagaagcgg accaggtcgc ggccgacggg ggaggtcttg
     3841 agataggcgt cgcggaacca ccgcagggcc tggacctggc cgacccaggc ggcgccgtag
     3901 gccgcggtgg cgaggaagca gaagccctcg acctgctgga acttctgggc ctcggtcgtg
     3961 atcgcggtcg agacgagggc gctggtgttg ccgcacttgt cctggtacgt gacgccgatc
     4021 tggtaggtgt agttgcccca cagctgctcg acgtcga
//
LOCUS       DASWNJ010001204        10178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_91554, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001204 DASWNJ010000000
VERSION     DASWNJ010001204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 10178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10178
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91554"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..989)
                     /locus_tag="VGB85_20295"
     CDS             complement(174..989)
                     /locus_tag="VGB85_20295"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY0136441.1"
                     /translation="MLLIDTHAHLDLPRFGDAKAQREAALRAQAADVGAVVIPGVEPA
                     SWDGMLATAARLRAEAPSVRFHTAFGIHPQVLPDLDSANDGEICAALAERVAAPPADM
                     VAIGECGLDFAADIVAGASRERQVAVLRAHFALARRTGLPLLLHCLRAHGPMLELLRA
                     EPLPPSILHSWSGSAEMARHFCKLGHYLSFAGALTLPRARRPLESARAVPADRLLIET
                     DSPDQTPYVRRPLRNEPAFLPDIAAALASARGEPLAEVAEYTTANARRLLSLP"
     gene            1197..1469
                     /locus_tag="VGB85_20300"
     CDS             1197..1469
                     /locus_tag="VGB85_20300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136442.1"
                     /translation="MTAEQAQAGEPSWVGEERWKPIVLEAVRLRQVANEPSVRILVGE
                     HAILIAGDEKHVVGVVFIKGHPVVKSVVRMVRQLMRPNSAAAAAIV"
     gene            1507..2931
                     /locus_tag="VGB85_20305"
     CDS             1507..2931
                     /locus_tag="VGB85_20305"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY0136443.1"
                     /translation="MDRVPDLLALRRDLVAAVGDERCSDAADDLAYYGADRCRGGWPV
                     RPSMIVFPRSIPEVQAVVRACAAHGVAIVPSGGRTGLAGAATATAGEVVLSLEKMQRV
                     LAVDVAARTLRCEPGLTLQGAHDAAAAHGLLYPIDYAAKGSAQIGGSLATNAGGVKVL
                     RYGLTRDWVLGLKAVIASGECLELGGALVKNNTGYDLRQLFIGSEGTLGVIVEVTLRL
                     CRPPADTLVALCAVADDAAVMALFTRLRASSLTLSAFEFLDDGCLRHVLTHRTGAPPF
                     TTPSPRYALVEIEIPGPGEAARAHAESELTALLGDATEAGEVDDALLAGTPAQARSLW
                     ALREDISESLHRHTPHKADIALPVARVGEFLAAWRPAVAAALPEVEALCFGHVGDGNL
                     HLNLLRPEALALPDFLARVHGFDDETYALVQAHGGSISAEHGVGLLKRDHLHYSRSPA
                     ELAIFRGIKAALDPAGLFNPGKIF"
     gene            3214..3295
                     /locus_tag="VGB85_20310"
     tRNA            3214..3295
                     /locus_tag="VGB85_20310"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3248..3250,aa:Leu,seq:tag)
     gene            3361..3843
                     /locus_tag="VGB85_20315"
     CDS             3361..3843
                     /locus_tag="VGB85_20315"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HEY0136444.1"
                     /translation="MIGTLIKRLTVRTLDAVVERGRESERPTLRFAANGLDRARGWVG
                     LDRIGRRTVSPPWDGSQPEQPMWQTDKDKLHKHRVDRGIIKTDGAQPPKPAEPAVKIY
                     IKRGCPYCRAAIDLVNERNIPFTQIDYSDDHDLRRAIRGQTGRKTSPHVFIHGKSIGG
                     "
     gene            3886..4239
                     /locus_tag="VGB85_20320"
                     /pseudo
     CDS             3886..4239
                     /locus_tag="VGB85_20320"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            4450..4686
                     /locus_tag="VGB85_20325"
     CDS             4450..4686
                     /locus_tag="VGB85_20325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEY0136445.1"
                     /translation="MIARVREVLEEVRPMVQSDGGDIELMDVIDDVVGVRLTGNCIGC
                     PSSQATLRQGIERKLKLRIPQIKGISSPQLTPLA"
     gene            4909..6732
                     /locus_tag="VGB85_20330"
     CDS             4909..6732
                     /locus_tag="VGB85_20330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136446.1"
                     /translation="MTTTTTTPQQRSSLLNIVSQPQVQSLLDEAKQRFSEEEGVSFRS
                     VNAHFKKPVERDFVAPQRAHTTLLFGGLTFRHEHLLKGVFESLGYKTAVVPTANVNAF
                     QLGKEYGNNGQCNPTYFTVGNLVQYVQHLRDVEGMTTQEIIDGYIFLTAGACGPCRFG
                     MYEAEYRLALRNAGFDGFRVLLFQQKGGLKQGDLEAGLKMDLDFFMGMLNAFNAGDLL
                     NEVANQVRAFETTTGATDKALAESTQYMYEVMKSKKPAQLEGKVADLLDGRKKAFDIA
                     EQAIKFLDQLTTDDYLEPLREVSARLDQIEVDRLQPKPIVKVTGEFWAQTTEGDGNFN
                     MFRFLTAEGAEIIVEPIATWIAYMLWQTKIKARDRKDLVEKGEEPKWYELGKRAALEA
                     GYLKKRAIAGFADTVFRREYARMVEALGGTAHEIVDMDEMERLAHDFYHSRSEGGEGH
                     LEVAKNIYYSHKYYAHMVLSLKPFGCMPSTQSDGAQSAVVNRFKDMIFLPIETSGEGE
                     INAHSRVQMALGEAKAKAKREFAEVLEGLPYSLEELRAYVARNPELKRPMYKFPRKTP
                     KVVGTAAHFAIHLTERMRAEGVISRRSAGPADVAAAEAAAQ"
     gene            6817..>10178
                     /locus_tag="VGB85_20335"
     CDS             6817..>10178
                     /locus_tag="VGB85_20335"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEY0136447.1"
                     /translation="MSQYKYMIGFDLGSTTVKAVVVDVASDEIIWKDYQRHESKQADR
                     ALKMLKQIEAEVPGLRAGNTRMFMTGSGGSNVGRWIGAKFVQEVNAVSLAVEKLHPEV
                     NSVIELGGQDAKIIIFKPDPETGRKKKIPSMNDKCAGGTGAVIDKVNAKLKIPAAELC
                     QQGYHGHKLHPVAGKCGVFAETDVNGLQKLGVPSNELMASLFESIIQQNLSVLTRGHT
                     LMPYVLLLGGPNTYIKGMVECWKANIPPIWAERGVELPPCDDPADLIIVPDNAQYFAA
                     LGAVEYGKTEDENVGVYTGIAKLEWYLTEGRLAEKKKAGGSGLSKNPEELASFMSDYT
                     PIKFTPKTFQPGEDVRVYLGIDGGSTSSKAVLIDEAGEVVKKVYQLSKGNPIVDTKEL
                     LADLREQVEGAGAKLTILGVGTTGYAKDILRDVLRADAAIVETVAHCESALHFYDNVD
                     VVCDVGGQDIKIIILKNGKVKDFKLNTQCSAGNGYFLQSTADGFGHDVRDYAKLAFDA
                     KAMPSFGYGCAVFMQSDIVDFQRQGWAPEEIMAGLANVLPKNIWLYVSQIPNLAKLGQ
                     RFVLQGGTQHNLAAVKAQVDFIQERFKAKGLEADVIVHKHCGESGAIGAALEVRRMHQ
                     QLGHQTEWIGIDKIDSIGFSQRRDESTRCYFCKNKCLRTFIDVDLEMQDKDAEKRMAL
                     GQLLKIRKKENSVDESIGTKRLIIATCEKGEVEDIESMKKIKGGLDALKKNYPNLCSK
                     AAKDIWDAQEPPRVADNIDDVGLGMREHLDLPPALAEHQGKVDELLKKVNRSPKVAGF
                     LGKAAEKLGQVASKIEVGGSIAAVATAARETLEAQQRRIELMKKRETLRIGIPRVLNQ
                     YSQNPFFSAYFESLGVSPKNIVYSDYTSEELYKEGSKRGAIDPCFPSKVCIAHMHNLL
                     EVKHKRRPLHVIVFPQVDSMETWLSGTVGSRACPTVVGSADTTRAAFVKESDIFAQKG
                     IKLVIPFVQMAERKLCKRQMFEYFKDILGLSEEENSRAVDEGFVAQDRFFVDHRKDGR
                     AIIEQLERDQKIGVVLLARPYHNDPGMNHEIPDELQKLGYPILTVQSLPLDEHIVGPL
                     FQADIDAGFIKDPFDVSDVWKNSYSENTNQKV"
BASE COUNT         1779 a         3635 c         3330 g         1434 t
ORIGIN      
        1 ggggtctcct cgaccacctc gaccgcctgg ctgaccggcg gcgcgaccac gggctcctcc
       61 ccgcaggcga ggccgacgag cgcgccgagg cagagccagc gaagatgggt ggggccgggc
      121 aaggtcggtg cgagggaagc accaagtcgg cggcgtcgca actaacccgg ggctcagggg
      181 agggacagca gccggcgggc gttggcggtc gtgtactccg cgacctcggc gagcggctcc
      241 ccgcgggccg aggccagcgc cgccgcgatg tcgggcagga acgccggctc gttgcgcagc
      301 ggccggcgca cgtacggggt ctggtcgggg ctgtcggtct cgatcagcaa cctgtccgca
      361 gggacagccc gcgccgactc gagcggtcgc cgcgcgcgcg gcagcgtgag cgcccccgcg
      421 aacgagaggt agtgcccgag tttacagaag tggcgcgcca tctcggcgct gcccgaccag
      481 ctgtgcagga tcgacggcgg caggggctcg gcgcgcagca gctcgagcat gggcccatgc
      541 gcgcgaagac aatgcagcag caacggaagc ccggtgcgcc gcgccagcgc gaagtgagcg
      601 cgcaacacgg cgacctgacg ctcgcgcgag gccccggcca cgatgtccgc ggcgaagtcg
      661 aggccgcact cgccgatcgc caccatgtcg gccggcggcg ccgcgacccg ctccgccagc
      721 gccgcgcaga tctccccgtc gtttgcagaa tcgagatctg gcagcacttg cgggtgaatt
      781 ccgaaggccg tgtgaaagcg caccgatggc gcttccgcgc gcagtcgagc ggccgtcgcc
      841 agcatcccat cccagctcgc cggctcgacc ccggggatca ccacggcccc gacgtcggcc
      901 gcctgggccc gaagggccgc ctcgcgctgg gcctttgcgt cgccgaagcg gggcaggtcg
      961 aggtgggcat gtgtgtcgat gagaagcatg gccgtcaagg tgcgacacga ggggcacgaa
     1021 acgttgatcc gcgacttgcg gaattgacgc acctagagcc tttttgtaca accactgccc
     1081 cggcgcccgc gacccccagg tccaggatta gaagccggtg aactcaggga aattccatgt
     1141 catcgacacg tattgagcgc gttcttcgcc acgacggcat cgtcgcggtc ctcgacatga
     1201 ccgcagagca ggcgcaagcc ggcgagccgt cgtgggtggg cgaagagcgc tggaagccga
     1261 tcgtgctgga ggcggtgcgc cttcggcaag tcgccaacga gccgtccgtc cgcatcctcg
     1321 tcggcgagca cgccatcctc atcgccggcg acgagaagca cgtcgtcggt gtggtgttca
     1381 tcaagggcca cccggtcgtg aagtccgtgg tgcgcatggt ccggcagctg atgcgcccga
     1441 actcggccgc agccgccgcg atcgtctaaa ccggcggacg gccccccgtc cccggacgtc
     1501 cacagcatgg accgcgtccc cgacctgctc gcactgcgtc gcgacctcgt cgcggccgtc
     1561 ggcgacgagc gctgcagcga cgccgccgac gacctcgcct actacggcgc cgaccgctgc
     1621 cgcggcggct ggccggtgcg gccgtcgatg atcgtgttcc cacgcagcat ccccgaggtc
     1681 caggcggtgg tccgggcctg cgccgcgcac ggcgtggcga tcgtgccctc gggcgggcgc
     1741 accgggctcg cgggcgccgc gacggcgacc gccggcgagg tcgtgctctc gctggagaag
     1801 atgcagcgcg tgctcgcggt cgacgtcgcc gcgaggaccc tgcgctgcga gcccggcctg
     1861 acgctgcaag gtgcgcacga cgccgccgcc gcgcacggcc tgctctaccc gatcgactac
     1921 gccgccaagg gctcggcgca gatcggtggc agcctcgcga ccaacgccgg cggcgtgaag
     1981 gtcctgcgct acggtctcac gcgtgactgg gtcctcgggc tcaaggccgt catcgcctcc
     2041 ggcgagtgcc tcgagctcgg cggggcgctc gtcaagaaca acaccggcta cgacctgcgc
     2101 cagctgttca tcggctccga gggcacgctc ggcgtgatcg tcgaggtcac gctgcgcctg
     2161 tgtcgcccgc ccgccgacac cctcgtggcc ctgtgcgccg tcgccgacga cgccgccgtc
     2221 atggccctgt tcacccgcct gcgtgccagc agcctgacgc tctccgcctt cgaattcctc
     2281 gacgacggct gcctgcgtca cgtgctcacg caccgcaccg gcgcgccgcc cttcaccacg
     2341 ccgagccctc gctacgcgct cgtcgagatc gagatccccg gccccggcga ggccgcgcgc
     2401 gcccacgccg agtccgagct gaccgccctg ctcggcgacg cgaccgaggc cggcgaggtc
     2461 gacgacgccc tgctcgccgg cacccccgcg caggcgcgca gcctctgggc cctgcgcgag
     2521 gacatcagcg agtccttgca tcggcacacg ccgcacaagg ccgacatcgc cttgccggtc
     2581 gcgcgcgtcg gcgagttcct cgcggcctgg cgcccggccg tcgcggccgc gctgccggag
     2641 gtcgaggcgc tgtgcttcgg tcacgtcggc gacggcaacc tgcacctcaa cctcctgcgc
     2701 cccgaggcgc tcgcgctgcc ggacttcctc gcgcgcgtgc acggcttcga cgacgagacc
     2761 tacgcgctgg tccaggccca cggcgggagc atctcggcgg agcacggcgt cggcctgctc
     2821 aagcgcgacc acctgcacta cagccgcagc cccgccgagc tggcgatctt ccgcggcatc
     2881 aaggccgccc tcgaccccgc cggcctgttc aacccgggca agatcttctg atcttcccgg
     2941 cggcgcgcag gccagcgagt cgcccgcgcc gtgatcgccg caaagcacga ccttcgcgtc
     3001 acgaccttct tcgcggccgg cgccgccttc gcgggcccgg gctgctcggc cacgacggga
     3061 acggcgtgct cggccacgac ggggacggcg tgctcggcca cgacgggggc ggcgtgctcc
     3121 gtggacggcc cgagccgcac accgtgagca tccggcagcc cctggagaac ggggtgtcga
     3181 cgcgcacggc ggcatgataa cgtccgcgct cccgcgagcc tggcgaaatt ggtagacgcg
     3241 ctggatttag gttccagtac cgcaaggtgt gtcggttcga gtccgacggt tcgcactcgc
     3301 gcggggtccc cgcagacctc cggcttgacg gcgtcgaacg aatgcgggta cccgtgaccc
     3361 gtgatcggga cccttattaa acgcttgacg gtgcgcacgc tggacgcggt ggtggagcgg
     3421 ggcagggagt cggagcggcc gacgttacgg ttcgccgcga acggcctcga ccgcgcgcgc
     3481 gggtgggtcg gcctcgaccg cattgggagg cgaacggtct cgccgccctg ggacggctcg
     3541 cagcccgagc agccgatgtg gcagaccgac aaggacaagc tgcacaagca ccgggtcgac
     3601 cgcggcatca tcaagaccga cggggcccag ccgcccaagc cggccgagcc cgccgtcaag
     3661 atctacatca agcgcggctg cccctactgc cgcgccgcga tcgacctcgt caacgagcgc
     3721 aacatcccgt tcacgcagat cgactacagc gacgaccacg acctgcgccg tgcgatccgc
     3781 gggcagaccg ggcgcaagac ctccccgcac gtcttcatcc acggcaagtc gatcggcggc
     3841 taagacgagc tgcgggagct cgaccagtcg ggtgccctca aggacatgct cgaaaagcca
     3901 ggcgccgtga aggtcgccgc gcccgtgttg ccgcccttcg acgaggagga cgacgacagc
     3961 gaggacgaga tctcggccca gggcctgcgc acgcgcgtca gcgagggcgc gaagctgctg
     4021 ctgctcgacg tccgcgagcc cgacgaacgc aaccgcaccg gcgtcctgcc acacgcggtc
     4081 ctgatcccgc tcggcgagct cgagcagcgc cacgccgagc tcgacccgac cggcgtgtgg
     4141 atcacctact gccaggctgg caagcgctcg ctgcaggccc aggcgatcct gcgcgagaag
     4201 ggctttggcc gggtcgtcag cctgcgtggg ggcatcgtcg cgtggaccga ggccggcggc
     4261 gccaccgtgg ccgcgagcca ggtcgacccc cacggcctcg tgcctgccgc cacctccgtg
     4321 agcgccgggc cgaccactgc cgtccgtcgc gtctcgctgc cggtgatcca cccggagcgc
     4381 tcgcccttcg aggatctgct gttcggcgac ccggccgagg ccgaggtcga gcacctcgtc
     4441 ggtgacgccc tgatcgcccg cgtacgcgag gtgctcgagg aggtccggcc gatggtccag
     4501 agcgacggcg gcgacatcga gctgatggat gtcatcgacg acgtcgtcgg cgtgcgcctg
     4561 accggcaact gcatcggctg cccgagctcg caggccaccc tgcgccaggg catcgagcgc
     4621 aagctcaagc tgcgcatccc gcagatcaag ggcatctcct cgccccagct gaccccgctg
     4681 gcctgaggaa aacgccgggg cgggccttat cgcccccacc cggctgataa aaagacgacc
     4741 gcggccacgc ctgcgatcgg ccctgcacgc ccgtggctgt ggccctgtgg cccctgcggc
     4801 tttttcttgc cggtcgcggc ggattatgcg tcactggccc aaagttgact gacacccttg
     4861 tcagtgacga gcgtgtcagc gaagtttgaa ctttcccgaa ggcccacgat gacgaccacc
     4921 acgacgaccc cgcaacagcg cagctccttg ctcaacatcg tgtctcaacc gcaggtgcag
     4981 tcgctgctcg acgaggccaa gcagcgcttc tccgaggagg agggcgtcag ctttcgcagc
     5041 gtcaacgcgc acttcaagaa gccggtcgag cgggacttcg tcgcgccgca gcgcgcgcac
     5101 acgacgctgc tgttcggtgg tctgaccttc cgccacgagc acctgctcaa gggcgtgttc
     5161 gagtcgctgg gttacaagac cgcggtggtg ccgacggcga acgtcaacgc gttccagctc
     5221 ggcaaggagt acggcaacaa cgggcagtgc aacccgacct acttcaccgt cggcaacctc
     5281 gtgcagtacg tccagcacct gcgcgacgtc gagggcatga ccacccagga gatcatcgac
     5341 ggttacatct tcctgaccgc cggcgcctgc ggtccgtgcc gcttcggcat gtacgaggcc
     5401 gagtaccggc tcgccctgcg caacgccggc ttcgacggct ttcgcgtgct gctgttccag
     5461 caaaagggcg gcctcaagca gggcgacctc gaggctggcc tcaagatgga cctcgacttc
     5521 ttcatgggca tgctcaacgc gttcaacgcg ggcgacctgc tcaacgaggt cgccaaccag
     5581 gtccgcgcct tcgagacgac caccggcgcg accgacaagg cgctcgccga gagcacccag
     5641 tacatgtacg aggtgatgaa gagcaagaag ccggcgcagc tcgagggcaa ggtcgccgac
     5701 ctgctcgacg gccgcaagaa ggccttcgac atcgccgagc aggcgatcaa gttcctcgac
     5761 cagctgacga ccgacgacta cctcgagccg ctgcgcgagg tgtccgcgcg cctcgaccag
     5821 atcgaggtcg accgcctcca gcccaagccg atcgtcaagg tgaccggcga gttctgggcc
     5881 cagaccaccg agggcgacgg caacttcaac atgttccgct tcctcaccgc cgagggcgcc
     5941 gagatcatcg tcgagccgat cgcgacctgg atcgcgtaca tgctgtggca gaccaagatc
     6001 aaggcgcgtg accgtaagga cctcgtcgag aagggcgagg agccgaagtg gtacgagctc
     6061 ggcaagcgcg ccgcgctcga ggccggttac ctcaagaagc gtgcgatcgc cggcttcgcc
     6121 gacaccgtgt tccgccgcga gtacgcgcgc atggtcgagg ccctcggcgg caccgcccac
     6181 gagatcgtcg acatggacga gatggagcgg ctcgcccacg acttctacca ctcgcgctcc
     6241 gagggcggcg agggccacct cgaggtcgcc aagaacatct actactcgca caagtactac
     6301 gcgcacatgg tgctgtcgct caagccgttc ggctgcatgc cgagcaccca gtccgacggc
     6361 gcgcagtcgg cggtcgtcaa ccgcttcaag gacatgatct tcctgccgat cgagacctcc
     6421 ggcgagggcg agatcaacgc ccacagccgc gtccagatgg cgctcggcga ggccaaggcc
     6481 aaggccaagc gcgagttcgc cgaggtgctc gagggcctgc cctacagcct cgaggagctg
     6541 cgcgcctacg tcgcccgcaa ccccgagctc aagcgtccga tgtacaagtt cccgcgcaag
     6601 accccgaagg tcgtcggcac cgccgcgcac ttcgcgatcc acctcacgga gcgcatgcgt
     6661 gccgagggcg tcatttctcg gcgttccgcc ggccccgcgg atgtcgccgc cgccgaagcc
     6721 gccgcgcagt gagccccggg ggcgcacgcg ggccctggcc ccgcgctgcg cccctcgttc
     6781 gcgtcagggc cgagtccttc cggagttctt tttcaaatgt ctcagtacaa gtacatgatc
     6841 gggttcgatc tgggttctac caccgtcaag gccgtcgtcg tcgacgtcgc cagcgacgag
     6901 atcatctgga aggactacca gcgtcacgag agcaagcagg ccgaccgcgc cctgaagatg
     6961 ctcaagcaga tcgaagccga ggtcccgggc ctgcgcgccg gcaacacgcg catgttcatg
     7021 accggctcgg gcggctcgaa cgtcggccgc tggatcggcg ccaagttcgt gcaggaggtc
     7081 aacgccgtct cgctcgcggt cgagaagctc caccccgagg tcaactccgt gatcgagctc
     7141 ggtggtcagg acgccaagat catcatcttc aagcccgacc cggagaccgg ccgcaagaag
     7201 aagatcccgt cgatgaacga caagtgcgcg ggtggtaccg gcgccgtcat cgacaaggtc
     7261 aacgccaagc tcaagatccc cgccgccgag ctgtgccagc agggctacca cggccacaag
     7321 ctgcacccgg tcgccggcaa gtgcggcgtg ttcgccgaga ccgacgtcaa cggcctgcaa
     7381 aagctcggcg tcccgtccaa cgagctgatg gcctcgctgt tcgagagcat catccagcaa
     7441 aacctgtccg tgctgacgcg cggccacact ctcatgcctt atgtgctgct gctcggcggc
     7501 ccgaacacgt acatcaaggg catggtcgag tgctggaagg ccaacatccc gccgatctgg
     7561 gccgagcgcg gcgtcgagct cccgccctgc gacgacccgg ccgacctgat catcgtcccc
     7621 gacaacgccc agtacttcgc ggcgctcggc gccgtcgagt acggcaagac cgaggacgag
     7681 aacgtcggcg tatacaccgg catcgccaag ctcgagtggt acctgaccga gggccgcctc
     7741 gccgagaaga agaaggccgg cggctccggc ctgagcaaga acccggagga gctcgcctcc
     7801 ttcatgtcgg actacacgcc gatcaagttc accccgaaga ccttccagcc gggcgaggac
     7861 gtgcgcgtct acctcggcat cgacggcggc tcgacctcct ccaaggcggt gctgatcgac
     7921 gaggccggcg aggtcgtcaa gaaggtctac cagctctcca agggcaaccc gatcgtcgac
     7981 accaaggagc tgctcgccga cctgcgtgag caggtcgagg gggccggcgc caagctgacg
     8041 atcctcggcg tcggcaccac cggctacgcc aaggacatcc tgcgcgatgt cctgcgcgcc
     8101 gacgcggcga tcgtcgagac ggtcgcccac tgcgagagcg cgctgcactt ctacgacaac
     8161 gtcgacgtcg tgtgcgacgt cggcggccag gacatcaaga tcatcatctt gaagaacggc
     8221 aaggtaaagg acttcaagct caatacccag tgctcggccg gcaacggcta cttcctgcag
     8281 agcaccgccg acggcttcgg ccacgacgtg cgcgactacg ccaagctcgc cttcgacgcc
     8341 aaggcgatgc cgagcttcgg ctacggctgc gccgtgttca tgcagtcgga catcgtcgac
     8401 ttccagcgcc agggctgggc gcccgaggag atcatggcgg gcctcgccaa cgtgctgccc
     8461 aagaacatct ggctgtatgt cagccagatc ccgaacctcg ccaagctcgg ccagcgcttc
     8521 gtgctgcagg gcggcaccca gcacaacctc gccgcggtca aggcccaggt cgacttcatc
     8581 caggagcgct tcaaggccaa gggcctcgag gccgatgtca tcgtccacaa gcactgcggc
     8641 gagtccggcg cgatcggtgc ggccctcgag gtccggcgca tgcatcagca gctcggccac
     8701 cagaccgagt ggatcggcat cgacaagatc gacagcatcg gcttctccca gcgccgcgac
     8761 gagagcaccc gctgctattt ttgtaagaac aagtgcctgc gcaccttcat cgacgtcgac
     8821 ctcgagatgc aggacaagga cgccgagaag cgcatggcgc tcggccagct gctcaagatc
     8881 cgcaagaaag agaatagcgt cgacgagtcg atcggcacca agcgcctgat catcgccacc
     8941 tgcgagaagg gcgaggtcga ggacatcgag tcgatgaaga agatcaaggg cggcctcgac
     9001 gcgctcaaga agaactaccc gaacctgtgc tccaaggccg ccaaggacat ctgggacgcc
     9061 caggagccgc ccagggtcgc cgacaacatc gacgacgtcg gcctcggcat gcgcgagcac
     9121 ctcgacctgc ccccggccct cgccgagcac cagggcaagg tcgacgagct gctcaagaag
     9181 gtcaaccgca gccccaaggt cgccggtttc ctcggtaagg ccgccgagaa gctcggccag
     9241 gtcgccagca agatcgaggt cggtggcagc atcgccgccg tcgccaccgc cgcgcgcgag
     9301 accctcgagg cccagcagcg ccgcatcgag ctgatgaaga agcgcgagac cctgcgcatc
     9361 ggcatcccgc gggtgttgaa ccagtactcg cagaacccgt tcttcagcgc ctacttcgag
     9421 tcgctcgggg tctcgccgaa gaacatcgtg tactccgact acacctccga ggagctgtac
     9481 aaggagggtt ccaagcgcgg cgccattgac ccctgcttcc cgtccaaggt gtgcatcgcc
     9541 cacatgcaca acctgctcga ggtcaagcac aagcgccggc ccctgcacgt gatcgtgttc
     9601 ccgcaggtcg actcgatgga gacctggctg tcgggcaccg tcggctcgcg cgcctgcccg
     9661 acggtggtcg gctccgcgga cacgacgcgc gccgcgttcg tgaaggagtc ggacatcttc
     9721 gcccagaagg gcatcaagct ggtgatcccg ttcgtgcaga tggccgagcg caagctgtgc
     9781 aagcgccaga tgttcgagta cttcaaggac atcctcggcc tgagcgagga ggagaacagc
     9841 cgcgcggtcg acgagggctt tgtcgcccag gaccgcttct tcgtcgacca ccgcaaggac
     9901 ggccgcgcga tcatcgagca gctcgagcgc gaccagaaga tcggcgtggt ccttctggcc
     9961 cggccgtacc acaacgaccc gggcatgaac cacgagatcc cggacgagct gcaaaagctc
    10021 ggctacccga tcctcaccgt gcagtcgctg ccgctcgacg agcacatcgt cggtccgctg
    10081 ttccaggccg acatcgacgc cggcttcatc aaagacccgt tcgacgtcag cgacgtgtgg
    10141 aagaacagct acagcgagaa caccaaccaa aaggtctg
//
LOCUS       DASWNJ010001205         2009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_587327, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001205 DASWNJ010000000
VERSION     DASWNJ010001205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587327"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VGB85_20340"
     CDS             <1..185
                     /locus_tag="VGB85_20340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136448.1"
                     /translation="GKGDGRISIADAQRLIAAVTDGGRYTDVEKETMAHIRENFKFTE
                     EADTFFRDEIRRFAAR"
     gene            complement(198..989)
                     /locus_tag="VGB85_20345"
     CDS             complement(198..989)
                     /locus_tag="VGB85_20345"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0136449.1"
                     /translation="MHVHREGPEHARTSVLLLHAGASSGAQWTGYARALGPDVRTLAP
                     DLHGHGETPLPADITVTGAIDRMVDDLLEVPGADRFHLVGHSFGGLLALALALRMPAR
                     VRSLTVVEPACFDALRVGGPRALHEESSHEVEHLTALITRGDHEQAMQLVVARWGMGR
                     WELFTPQQRAQFVALAPALLGIGLVAAWRWRCEPAELARLAGIPTVLVHGEHSPAVAF
                     HVSENLCGLIPGARRECIAGAGHMLPLTHRAALRRLLQRQLEPGA"
     gene            complement(998..>2009)
                     /gene="sppA"
                     /locus_tag="VGB85_20350"
     CDS             complement(998..>2009)
                     /gene="sppA"
                     /locus_tag="VGB85_20350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEY0136450.1"
                     /translation="GYRDELMAHVRDKAGEDAELLFHDKYLARAGKPHTRGKRIAVVY
                     GVGPVMRGGNSFDPLFGGGPTMGSKTVAGALREAIDDEDVKAIIFRVDSPGGSYVASD
                     TIWRETVRARQLGKPVIVSMGNVAGSGGYFVAMNATKIVAHPATITGSIGVLGGKMLT
                     RKLFEKLGVTYGTIKTAANADMFSELDDYSAHGWQRFQAWLDRVYLDFTSKVAEGRGL
                     PLAKVQEIAKGRIWSGEDAKQIGLVDELGGWAVATRLAREAAGIGADEDIELRTYPKD
                     RSLFEQVLGGGRDNSDAPTSIHAQVELMEQLRPVAAELRALGLGPGNTGVLGMPALEV
                     EP"
BASE COUNT          274 a          694 c          757 g          284 t
ORIGIN      
        1 ccggcaaggg cgacggccgc atctcgatcg cggacgccca gcgcctgatc gcggcggtca
       61 ccgacggcgg ccgctacacc gatgtcgaga aggagacgat ggcgcacatc cgcgagaact
      121 ttaagttcac cgaggaggcc gacaccttct tccgcgacga gatccgtcgc ttcgcggcgc
      181 gctgagtcgt ccggtcgtca cgcgccgggt tcgagctgcc gctgcaacag cctgcgcagc
      241 gcggcccggt gcgtgagcgg caacatgtgg cccgcgcccg cgatgcactc gcgccgggcc
      301 cccgggatca agccgcacag gttctcgctg acgtggaagg cgacagccgg gctgtgctcg
      361 ccgtggacca gcacggtcgg gatcccggcg aggcgcgcca gctcggcggg ctcgcagcgc
      421 cagcgccagg cggcgaccag gccgatcccg agcagcgccg gggcgagggc gacgaactgg
      481 gcccgctgct gcggcgtgaa cagctcccag cggcccatgc cccagcgcgc gacgaccagc
      541 tgcatcgcct gctcgtgatc gccgcgggtg atcagggcgg tcaggtgctc gacctcgtgg
      601 ctgctctcct cgtgcagcgc ccgcgggccg ccgacgcgca aggcatcgaa gcaggccggc
      661 tcgacgacgg tgaggctgcg gacgcgggcc ggcatccgca gcgcgagggc gagggcgagc
      721 aggccgccga acgagtgacc gacgaggtga aagcggtcgg cgccggggac ctcgagcagg
      781 tcgtcgacca tgcggtcgat cgcgccagtg acggtgatgt cggcgggcag gggggtctcg
      841 ccgtggccgt gcaggtcggg cgcgagggtc cggacgtcgg ggccgagcgc gcgcgcgtag
      901 ccggtccact gcgcgccgga gctggccccc gcgtgcagta gcagcacgct cgtgcgagcg
      961 tgctcggggc cctcccggtg gacgtgcatc accacgctca gggctcgact tcgagggccg
     1021 gcatgccgag cacgccggtg ttgccggggc cgaggcccag cgcgcgcagc tcggccgcga
     1081 cggggcgcag ctgctccatc agctcgacct gggcgtggat cgaggtcggg gcgtcgctgt
     1141 tgtcgcgacc gccgccgagg acctgctcga acagcgacct gtcttttgga taggtgcgca
     1201 gctcgatgtc ctcgtcggcg ccgatccctg cggcctcgcg ggcgagccgg gtggcgacgg
     1261 cccagccgcc gagctcgtcg accaggccga tctgcttcgc gtcctcgccg gaccagatgc
     1321 ggcccttggc gatctcctgg accttggcga gcggcagccc gcggccctcg gcgaccttgc
     1381 tggtgaagtc gaggtagacg cggtcgagcc aggcctgaaa gcgctgccag ccgtgcgcgg
     1441 agtagtcgtc gagctcgctg aacatgtcgg cgttggcggc ggtcttgatc gtgccgtagg
     1501 tgacgccgag cttctcgaac agcttgcgcg tcagcatctt gccgccgagc acgccgatcg
     1561 agcccgtgat cgtggcgggg tgggcgacga tcttggtcgc gttcatggcg acgaagtaac
     1621 caccggagcc ggcgacgttg cccatgctga cgatcacggg cttgccgagc tgccgggcgc
     1681 gcaccgtctc gcgccagatc gtgtcgctgg cgacgtacga gccgccgggg gagtcgacgc
     1741 ggaagatgat cgccttgacg tcctcgtcgt cgatcgcctc gcgcagggcg ccggcgacgg
     1801 tcttcgagcc catcgtcggg ccaccgccga acagcgggtc gaagctgttg ccgccgcgca
     1861 tcaccgggcc gacgccgtag acgacggcga tccgcttgcc gcgggtgtgc ggcttgccgg
     1921 cgcgcgcgag gtacttgtcg tggaacagca gctcggcgtc ctcgccggcc ttgtcgcgca
     1981 cgtgggccat cagctcgtcg cggtagccg
//
LOCUS       DASWNJ010001206         5556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_333979, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001206 DASWNJ010000000
VERSION     DASWNJ010001206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5556
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333979"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VGB85_20355"
     CDS             complement(<1..591)
                     /locus_tag="VGB85_20355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0136451.1"
                     /translation="MTPQIAVLDDEARMTEVLAMVLRREGHEVRCFTDPHACLAEVAQ
                     GGFDLLFTDLKMPGMDGLAVLKEARRLAPDMPVVLMTAHASVQTALAAVRAGAFDYVE
                     KPFDNETCKALAQRALEVTRLQRDNRYLRARLRQLHADDEVVTASPAMREVFALAARA
                     ARSRATALISGESGTGKELVARAIHRHSDRVQGPFVA"
     gene            complement(588..1553)
                     /locus_tag="VGB85_20360"
     CDS             complement(588..1553)
                     /locus_tag="VGB85_20360"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0136452.1"
                     /translation="MAPPRTRGDAAKPRVMAGKPLFGAAFGLGVGLLDYFVFRALGVQ
                     MSLGGHDLMLAICGLFAVTYAVLGYILGRLAAAQAAVRASAALVVESQRRALQSEKLA
                     EIGQLAAGVAHEVRNPLGVIRSSAAILLEDLPAGSDAARAGSFICEEVDRLNAVVRGL
                     LDYARPLAPRHERLPLAQLMTAVRPLVEDSLRGQGVTLATGASEAQVAGDPDLLAQLL
                     LTLIENAAQACAPGGRISVSVVREGDAVELRVADDGPGVDPAIAGQIFEPFFTTRAEG
                     TGLGLALAARVAQAHGGQLSLRTGAGLGPGGRGACFALRLPGGDR"
     gene            1792..2088
                     /locus_tag="VGB85_20365"
     CDS             1792..2088
                     /locus_tag="VGB85_20365"
                     /inference="COORDINATES: protein motif:HMM:NF022643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2834 domain-containing protein"
                     /protein_id="HEY0136453.1"
                     /translation="MNRKQIILAVFALDFLLLTAWAIVQHGYLGIFAHAFQNSATILL
                     AVDLVLAIVMIAGWMWVDANRHGLRVWPYLLVTALFGSAGPLLYLIRRESALKP"
     gene            2189..3538
                     /locus_tag="VGB85_20370"
     CDS             2189..3538
                     /locus_tag="VGB85_20370"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HEY0136454.1"
                     /translation="MLTARRSLARLSTLAALGLLACGPTTPTKPDEKPATPAMPATPD
                     TRPTPPDTQPEISEPDAAQVNPPSAETRAGVADSINAFAVDLHRALASRPGNLFVSPA
                     SIAIAFAMTHAGARGETGQEIARVFHFGGDATKTREGFAAALAGWSAPQEDLELSLAN
                     RLFGEKTVKFEPAFVDLTRTIFAAPLEPTDFKGAAEPARAHINAWVASKTHDKIADLL
                     PGGSVDPTTRLVLVNAVYFKASWRTPFAEGLTRSADFHTATGKRSVKMMEKTEHFAVH
                     VDAKARLKVLELPYMSTFSLVIVLPDAHDGLAAVEQALDATTFQRWIAALREQRVTVK
                     LPRFKIDPGEGLRLSGVLAGLGLRTAFDLNTADFTGMAPRSEQIVLSEAFHKAFIAVD
                     EKGTEAAAATAVMMASGGAPPSGEPLAFTADHPFLFMICDTRSGAILFMGRLEDPPA"
     gene            complement(3507..4910)
                     /locus_tag="VGB85_20375"
     CDS             complement(3507..4910)
                     /locus_tag="VGB85_20375"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEY0136455.1"
                     /translation="MGLRWATCGFVACIAGCAPWRADARTWGPEQAGISVELRAQARP
                     LFAGGQIDVEPLWAMTREARIIGIGEATHGTREFRELMNAMVRRAAQDERPLVVALEL
                     AFTQGLMLDAWVGDRWYALDPGLRGRNFDEVIGVGWLYATEEFRALFTWIRAYNETAA
                     PGREIRVVGADVCLHPSCVAGIVAYLAAVDPRYDDRARALLAPMKHLMVDLRRSPALA
                     AEARAGLAAVRARLEAQRDAYVAMHGESAHHDALRLVWLGERRIDAAIAGEGGSPTAD
                     RDWIMADTVGWIAQGRPDARIIVLAHDNHLTRDLYRLPNGRLTQGPVMGHELARRYDQ
                     DYVAVRTTFASGSFLAHHTRSTLATRRIRAGALRVFAVGTAPSGTLEATLRGPGDGYV
                     LDVRSAAAGEGPLARYLRGWHHTRSHGYAWRRALAAVPVAWTTLVPATSTDILVYFTR
                     TSATTPYLRPGGPRAGP"
     gene            complement(4943..>5556)
                     /locus_tag="VGB85_20380"
     CDS             complement(4943..>5556)
                     /locus_tag="VGB85_20380"
                     /inference="COORDINATES: protein motif:HMM:NF037202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="HEY0136456.1"
                     /translation="FGPEELLAYVYALLSAPGYTSRFAADLRGSGPRVPLVRDRSAWA
                     ATVALGRRAIALHCFTAGVSGAARCVQAPARQPDAVRHVDGALKFLVGGTCIGEYSPV
                     SAEVWAYSLSKYRPVAGWISGRLAGRVGRRSSRLDAIHCEQWTAAMNAELLELLWTVE
                     ATLALAPALDEALAIACAGPCWLADELPLPTDAERREPELPGR"
BASE COUNT          854 a         2258 c         1688 g          756 t
ORIGIN      
        1 cgccacgaac ggcccctgca cccggtcgct gtgccggtgg atcgcgcgcg cgacgagctc
       61 cttgccggtg ccgctctccc cggagatcag cgccgtcgcc cggctgcgag ccgcgcgggc
      121 cgccagcgcg aacacctcgc gcatcgccgg gctcgccgtc accacctcgt cgtcggcgtg
      181 cagctgccgc aggcgcgccc gcagataccg gttgtcacgc tgcagccgcg tcacctcgag
      241 ggctcgctgc gcgagcgcct tgcaggtctc gttgtcgaac ggcttctcga cgtaatcgaa
      301 ggcccccgcg cgcaccgccg ccagcgccgt ctgcacgctc gcgtgcgccg tcatcagcac
      361 cactggcatg tcgggcgcga gccggcgcgc ctccttgagc accgcgagcc cgtccatgcc
      421 tggcatcttg aggtcggtga acagcaggtc gaagccgccc tgtgcgacct cggcgaggca
      481 cgcgtgcggg tcggtgaagc agcgcacctc gtggccctcg cggcgcagca ccatcgccag
      541 cacctcggtc atgcgcgcct cgtcgtcgag caccgcgatc tgcggcgtca tcggtcgcct
      601 ccggggaggc gcaacgcgaa gcaggccccg cgcccgccgg gcccgaggcc cgcacctgtc
      661 cgcaaggaca gctgcccgcc atgcgcctgt gcgaccctcg cggcgagcgc gagccccagc
      721 cccgtgccct ccgcgcgcgt cgtgaagaac ggctcgaaga tctgccccgc gatcgccgga
      781 tcgaccccgg gcccgtcgtc ggcgacgcgc agctcgaccg catcaccctc tctcacgacc
      841 gacaccgaga tcctcccgcc cggcgcgcac gcctgcgccg cgttctcgat cagcgtcaac
      901 agcagctgcg ccagcaggtc cgggtcgccg gcgacctgcg cctcgctcgc gccggtcgcc
      961 agcgtcacac cctggcctcg caggctgtcc tcgacgagcg ggcgcacggc cgtcatcagc
     1021 tgcgcgagcg gcagtcgctc gtgtcgcggc gccagcggcc gcgcgtagtc cagcagcccg
     1081 cggaccaccg cgttgagccg gtcgacctcc tcgcagatga agctgcccgc gcgcgccgca
     1141 tcactgcccg cgggcagatc ctcgagcagg atcgccgccg acgagcggat cactcccagc
     1201 ggattacgca cctcgtgggc gacgcccgcc gcgagctgcc cgatctccgc cagcttctcg
     1261 ctctgcagcg cgcgccgctg gctctccacc accagcgccg cgctcgcgcg caccgccgcc
     1321 tgggccgccg cgagccgacc gaggatgtag ccgagcaccg cataggtgac cgcgaacagc
     1381 ccgcagatcg ccagcatcag gtcgtgcccg ccgagcgaca tctgcacgcc gagcgcacga
     1441 aacacgaagt agtcgagcag cccgaccccg agcccgaacg ccgcgccgaa cagcggcttg
     1501 ccggccatga cccgcggctt cgcggcgtcg cctcgtgtgc gcggtggagc catcaggagg
     1561 tcgagagtgt acccgcggtg tggcatcccc ggccagcaag caccacgcgt gccagtcgcc
     1621 gcggccacct gcgcgccgct ttcgcggccg tggcacgctg cgggtctgca acgcacgttg
     1681 cagccttgca acgtcgcgtc acctcgcgcg acgaacaact caatctccgc gccctcgcgg
     1741 cgctggcacg cgggttgctc atggtctccc cgtacccgca acggagccac catgaatcgc
     1801 aagcagatca tcctcgccgt gttcgccctc gacttcctcc tcctgaccgc ctgggcgatc
     1861 gtccagcacg gttacctcgg gatcttcgcc cacgccttcc agaactccgc gacgatcctg
     1921 ctcgccgtcg acctcgtgct ggcgatcgtc atgatcgccg gctggatgtg ggtcgacgcc
     1981 aatcgccacg ggctgcgcgt gtggccctac ctcctcgtca ccgcgctgtt cggctcggcc
     2041 gggccgctgc tctacctcat ccgccgcgag tccgccctca agccatgaac gatgtcagcg
     2101 tgcagtagcg cgccacaggc accccgcgat gacagcgatc gcggggtgcc tcgcttctca
     2161 acgctcgtgt gccgcggtaa accgggctgt gctcacggcc cgccgctccc tcgcccgcct
     2221 gtcgaccctc gccgcgctcg gcctgctcgc ctgcggcccg acgacgccca cgaagcccga
     2281 tgaaaaacca gccactcccg ccatgcccgc gacgcccgac accaggccca cgccaccaga
     2341 cacgcagccc gagatctccg agccggacgc cgcgcaggtg aacccaccga gcgccgagac
     2401 ccgcgccggt gtggccgaca gcatcaacgc attcgcggtc gacctgcacc gcgccctcgc
     2461 ctcgcgtcct ggcaacctct tcgtctcacc cgcgagcatc gctatcgcct tcgccatgac
     2521 ccacgccggc gcccgcggcg agaccgggca ggagatcgcc cgcgtgttcc acttcggcgg
     2581 cgacgccacg aagacccgcg agggcttcgc ggccgcgctc gccggctgga gcgccccgca
     2641 agaggacctc gagctctcgc tcgccaatcg tctgttcggc gagaagaccg tgaagttcga
     2701 gccggccttt gtcgacctca cgcgaacgat cttcgccgcc ccgctcgagc cgaccgactt
     2761 caagggcgcc gccgagcccg cgcgcgcgca catcaacgcc tgggtcgcca gcaagaccca
     2821 cgacaagatc gccgacctcc tgcccggtgg cagcgtcgac ccgaccaccc gcctcgtgct
     2881 cgtcaacgcc gtctacttca aggccagctg gcgcaccccc ttcgccgagg gcctgacccg
     2941 cagcgccgac ttccacaccg ccaccggcaa acgttcggtg aaaatgatgg agaagaccga
     3001 gcacttcgcc gtccacgtcg acgcgaaggc tcgcctcaag gtgctcgagc tgccgtacat
     3061 gagcaccttc tcgctggtga tcgtgctccc ggacgcccac gacggcctcg ccgcggtcga
     3121 gcaggcgctc gacgccacga cttttcaaag atggatcgcc gcgttgcgcg agcagcgggt
     3181 gaccgtcaag ctgccgcgct tcaagatcga ccccggcgag ggcctgcgcc tgtcgggcgt
     3241 gctcgccggg ctcggcctgc gcaccgcctt cgacctcaat accgccgact tcaccggcat
     3301 ggccccacgc tccgagcaga tcgtgctgtc cgaggcgttt cacaaggcct tcatcgccgt
     3361 cgacgagaag ggcaccgagg ccgctgcagc caccgccgtc atgatggcgt ccggcggcgc
     3421 cccaccctcc ggcgagccgc tcgccttcac cgccgaccac cccttcctgt tcatgatctg
     3481 cgacaccaga tccggcgcga tcctgttcat gggccggctc gaggaccccc cggcctgaga
     3541 tatggtgtcg tcgccgacgt ccgcgtgaag tacaccaata tatcggtgct cgtcgccggc
     3601 acgagggtgg tccatgccac cggcaccgcc gcgagcgccc gcctccacgc gtacccgtgc
     3661 gaacgcgtgt ggtgccagcc tcgcagatag cgggcgagcg ggccctcacc tgcggcggcg
     3721 ctgcgaacgt cgagcacata tccatctccc gggccgcgaa gcgtcgcctc cagggtgccg
     3781 ctcggcgccg tcccgaccgc aaagactcgc agcgcacctg cgcggatccg acgcgtcgcc
     3841 agggtgctcc gggtgtggtg cgcgaggaag ctgccgctgg cgaaggtcgt acgcaccgcg
     3901 acataatctt gatcgtaccg tcgcgccagc tcatggccca tcacggggcc ctgcgtcagc
     3961 cgcccattgg gcaatcgata cagatcacgc gtcaggtgat tatcgtgcgc gagcacgatg
     4021 atccgcgcat cggggcgccc ttgcgcgatc cagccgaccg tatcggccat gatccagtcg
     4081 cgatcggccg tcggcgatcc tccttccccg gcgatcgcgg cgtcgatccg gcgctcaccg
     4141 agccacacca gccgcagcgc gtcatgatgg gccgactcgc cgtgcatcgc gacatacgcg
     4201 tctcgctgcg cttcgagccg cgcccgcacc gcggccagac cggcgcgcgc ctcggcggcc
     4261 agcgcgggcg agcgacgaag atccaccatg agatgcttca tcggagccag cagcgcccga
     4321 gcccgatcgt cgtaccgagg atcgaccgcc gcgagatacg ccacgatacc ggccacacac
     4381 gacggatgca ggcacacgtc ggccccgacg acccggatct cccgccccgg cgcggccgtc
     4441 tcgttgtatg cgcggatcca cgtgaacagc gcgcgaaact cctcggtggc atacagccag
     4501 ccgacgccga tcacctcatc aaagtttcgc ccacgcaacc ccgggtccag cgcgtaccag
     4561 cggtccccga cccacgcgtc gagcatcagc ccctgagtaa acgccagctc gagcgcgacc
     4621 accagcggtc gctcatcctg cgcggcccgc cgcaccatcg cgttcatgag ctccctgaat
     4681 tctcgggtcc cgtgggtcgc ctcaccgatg ccgatgatgc gcgcctcccg ggtcatcgcc
     4741 cacagcggct cgacgtcgat ctgcccaccc gcgaacagcg gccgggcctg cgcccgcagc
     4801 tccaccgaga tccccgcctg ctccggcccc cacgtacgcg catcggctcg ccacggcgcg
     4861 cagccggcga tgcacgccac aaagccacac gtcgcccagc ggagccccat ggtgcccgta
     4921 ccgacgattc gaccccgtta tttcagcgcc ccggcaattc cggctcgcgc cgctcggcgt
     4981 cggtcggcag cggcagctcg tccgccagcc agcacggccc agcgcacgcg atcgccagcg
     5041 cctcgtcgag cgcaggcgcc agcgccagcg tcgcctcgac cgtccacagc agctcgagca
     5101 gctccgcgtt catcgccgcg gtccactgtt cgcagtggat cgcgtcgagc cgcgaggatc
     5161 gccgcccgac cctgcccgcc agccgaccgg agatccaccc cgccaccggt cgatattttg
     5221 acagcgaata tgcccacact tccgccgaca ccggcgaata ctccccgata cacgtaccac
     5281 cgacaagaaa cttcagcgca ccatcgacgt gccgcaccgc gtccggctgc cgcgccggtg
     5341 cctgcacgca gcgcgccgcc cccgacacgc ccgccgtaaa acagtgcagc gcgatcgccc
     5401 gccgccccag cgccacagtc gccgcccacg ccgaccgatc ccggaccagc ggcacccgcg
     5461 gcccgctccc ccgcaggtcc gccgcgaacc tgctcgtata ccccggcgcc gacagcagcg
     5521 catacacata ggccagcagc tcctccggcc cgaacg
//
LOCUS       DASWNJ010001207         2864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_91621, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001207 DASWNJ010000000
VERSION     DASWNJ010001207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91621"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1312)
                     /locus_tag="VGB85_20385"
     CDS             complement(<1..1312)
                     /locus_tag="VGB85_20385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136457.1"
                     /translation="MAKKKSKPGTTIDIDKELTVESLLARAEGLEVERLARAASERDK
                     PRATRRSEAVRPSNPVHWGYPYNFDGALPADERAALIAFFERFTTVSGSRSGFFIAGP
                     GTTPETRRAAWDGGATVLEVDQLRAQMPPDDFTARLDRLIAVLAQPGYQAWFEVAMLL
                     STWDAATLPDAIAEATRGLESWPDELRTMIEPWQRRPELRALVRVRWGDLDGDDDVGR
                     ITTIRSQDADGLERNQERFSHVTALDMSGKPDLPELVIRCTGLTRLERLLLQQPTYTS
                     GTAKLSLGRLLAAPHLQGLKALSLYGYTLAAADLKALAAGPQPLEHLRIQYARLKPTA
                     GKALAQLASRRQLRTLDLKYNDLGPKGAAALFGDPDDWRSLRVLDISANEIGDGGVEA
                     LVAASLEELRWLNISSNDSKQQLTAASGHALASAASLGRLETLNL"
     gene            1721..>2864
                     /locus_tag="VGB85_20390"
     CDS             1721..>2864
                     /locus_tag="VGB85_20390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEY0136458.1"
                     /translation="MRPFTPALSLLLLTLPACAARDDGDAEACIVERFELQPHDRFVL
                     GETYWLPRLDDGADCKSRAWEVVQAPTDSPDELVAGADGLTRFTPTTTGEHSFGLGGE
                     ALVSITVVPSAGRPFHNLNYYPSRSVARVGEELWVADVYSPTVTRLDPASLAVRGRVE
                     VGPWPVALAWQEGMTRALVVQRGGDTLGIVDIASNRLVDAVWVGDEPSNVVVSADGKT
                     AWVALATEAAIAVVDVETAAVTARIATVQDPLALALAPDGSRLYVASHRSGHPSRFPF
                     AADPVAAEFDLDIIDTASSAVVKRVLDVGTTITGLLVAPDGKRLYVATVRNDTEISQL
                     EAPAFQNMVLVLDAATGAELAATDLDVQDTSAGAAAAPHQLALAGDK"
BASE COUNT          376 a         1015 c         1058 g          415 t
ORIGIN      
        1 acaggttgag ggtctccagc cggccgagcg acgcggcgga cgcgagggcg tggcccgagg
       61 cggcggtcag ctgctgcttc gagtcgttcg acgagatgtt gagccagcgc agctcctcga
      121 gcgacgcggc gacgagcgcc tcgacgcccc cgtcgccgat ctcgttggcg gagatgtcga
      181 ggacccgcag cgaccgccag tcgtcggggt cgccgaacag ggcggccgcg cctttcggtc
      241 cgaggtcgtt gtatttgagg tcgagggtcc gcagctgccg gcgcgacgcg agctgggcga
      301 gcgccttgcc ggcggtcggc ttgagcctgg cgtactggat ccgcaggtgc tcgagcggct
      361 gcgggccggc ggcgagcgcc ttgaggtcgg cggccgcgag cgtgtagccg tacaacgaca
      421 gcgccttgag gccctggagg tgcggcgcgg ccagcagccg gcccagcgac agcttcgcgg
      481 tgccggaggt gtaggtcggc tgttgcagga ggagccgctc gagccgcgtc agcccggtgc
      541 agcggatcac gagctcgggc aggtcgggct tgccggacat gtcgagcgcg gtcacgtggg
      601 agaagcgctc ttgattgcgc tcgaggccgt cggcgtcctg gctgcggatc gtcgtgatgc
      661 ggccgacgtc gtcgtcgcca tcgaggtcgc cccagcgcac gcggacgagc gcgcgcagct
      721 cggggcggcg ctgccacggc tcgatcatcg tgcgcagctc gtcgggccag gactcgagcc
      781 cgcgcgtggc ctcggcgatc gcgtccggca gcgtcgccgc gtcccaggtc gagagcagca
      841 tcgcgacctc gaaccatgcc tggtaccccg gctgcgcgag cacggcgatg aggcgatcga
      901 ggcgggcggt gaagtcgtcg ggcggcatct gggcgcgcag ctgatcgacc tcgaggacgg
      961 tggcgccgcc gtcccacgcg gcgcggcgcg tctcgggcgt ggtcccgggg ccggcgatga
     1021 agaagcccga gcggctcccg gagacggtcg tgaagcgctc gaagaacgcg atcaatgcgg
     1081 cgcgctcgtc cgccggcaag gcgccgtcga agttgtacgg gtagccccag tgcacggggt
     1141 tgctcggacg gaccgcctcc gaccgacgcg tcgcgcgggg cttgtcgcgc tcgctggcgg
     1201 ctcgcgccag gcgctcgacc tcgaggccct cggcgcgggc gagcaggctc tcgacggtca
     1261 gctccttgtc gatgtcgatg gtcgtgccgg gcttcgactt cttcttcgcc atggacgccg
     1321 agcttgccgc gcgcggcggg cggcgtcacc tgctcatcga ctcacggttc gtcatcgtcg
     1381 gcggcgccga tgagctcgca actccgcgtc gcagcgccgg cgcaggtcga ggtcgtggca
     1441 ccgctcgacc ggcgcacgga tcgagatcca gcatcgcgcg agccgcatat gtcgagcgta
     1501 ccagcggcct gcatcctgcc tgcgagcgcg tgtcgaccgg gggcggcagc ccgcatgatc
     1561 gcggtgtgtc gatgtgcgtc gttcaggtca cgcgcggacg cgagcgagac gcgcgacaga
     1621 tcggccgacc agtgctattc gcccctcgcc tggccggtag atccgtccaa ggcgtccgtt
     1681 tgcctacttc gcggtgaccc ccgcgcgccc tggagactcc atgcgtccgt ttacacctgc
     1741 cctgtccctg ctgctgctga ccctgcctgc ctgtgccgcc cgcgacgacg gcgacgccga
     1801 ggcgtgcatc gtcgagcgct tcgagctgca accacacgac cgcttcgtgc tcggtgagac
     1861 ctactggctg ccgcgactcg acgacggcgc ggactgcaag tcccgcgcgt gggaggtcgt
     1921 gcaggccccg accgacagcc ccgacgagct ggtcgccggc gccgacgggc tgacccgctt
     1981 cacgccgacg accaccggtg agcacagctt cggcctcggc ggcgaggcgc tggtcagcat
     2041 caccgtcgtg ccctccgcgg gccggccgtt tcataacctc aactattatc cgagccgctc
     2101 ggtcgcgcgg gtcggcgagg agctgtgggt cgccgacgtg tacagcccga cggtcacgcg
     2161 cctcgacccg gccagcctcg cggtgcgtgg tcgcgtcgag gtcgggccgt ggccggtcgc
     2221 gctggcctgg caggagggca tgacgcgcgc gctggtggtt cagcgcggcg gcgacactct
     2281 cggcatcgtc gacatcgcca gcaaccggct ggtcgacgcg gtgtgggtcg gcgacgagcc
     2341 gagcaacgtg gtcgtcagcg ccgacggcaa gaccgcctgg gtcgcgctcg ccaccgaggc
     2401 cgcgatcgcg gtcgtcgacg tcgagacggc ggcggtgacg gcccggatcg ccaccgtgca
     2461 ggacccgctg gcgctggcgc tcgcgcccga cggcagccgc ctgtacgtcg ccagccaccg
     2521 ctccgggcac ccgagccgct tccccttcgc ggccgacccg gtggccgcgg aattcgatct
     2581 cgacatcatc gacactgcca gcagcgcggt cgtcaagcgc gtgctcgacg tcggcacgac
     2641 gatcaccggc ctgctcgtgg cgccggacgg caagcggctg tacgtcgcca ccgtccgcaa
     2701 cgacaccgag atctcgcagc tcgaggcgcc ggcgtttcag aacatggtcc tcgtgctcga
     2761 cgccgccacc ggcgcggagc tggcggcgac cgacctcgac gtccaggaca cctcggcggg
     2821 cgccgccgcg gcgccgcacc agctcgcgct cgcgggcgac aagc
//
LOCUS       DASWNJ010001208         2688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_404500, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001208 DASWNJ010000000
VERSION     DASWNJ010001208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404500"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VGB85_20395"
     CDS             <1..240
                     /locus_tag="VGB85_20395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136459.1"
                     /translation="GIGRYHGDEGLLSFMNPKAILVDRGKADYDPIWFPHKDKLGHAL
                     NVFRGVASKKLTSVIKGFLGMRKVAADAHKPDGDK"
     gene            293..1966
                     /locus_tag="VGB85_20400"
     CDS             293..1966
                     /locus_tag="VGB85_20400"
                     /inference="COORDINATES: protein motif:HMM:NF012435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEY0136460.1"
                     /translation="MTEVPGAKKERWGTRVGLVLAVAGNAVGLGNFLRFPAQAAQNGG
                     GAFLIPYLISFLLMGLPLLWVEWAIGRHGGQYGHHSAPGMFARMGKSPWLKYVGVFGL
                     FTNLTVAAYYCYIESWTLAYVWHSLAGTFVSTPATEFFPDYLGTTNASITAIPYEAAG
                     WFVATLALNTYILSRGLVKGVELAAKIGMPLLLLFGMVLAIRGLTLGPGDPGVIESPL
                     AGLNFVWQPQTSGLWNPSTWLAAAGQVFFTLSVGMGSIHCYAAYLRERDDIALNATTA
                     GSVNEFVEVILGSAILIPIATAYLGLNLVQAATATGSGFDLAFLTLPTLFHQWGAFGP
                     IACAMWFGLLFFAGITSSLAMGQPVVAFLQDEFEVRRSPAAAAFGTATLVLGLMCVIL
                     YPGGTFAEFDFWTGTFSLIIFAFAEVIIFGWIFGIERGWAEIQRGADLRLPEFFKYVI
                     KYVTPAFIGVVLLGALIKPVGSWGDAMGSLISAGHWPLDPGSVIGKVLLVGATESWFD
                     AQGNITKVAVEDITRVVLITVFVLIALLVRKAWKGRVIVHERQEGNVKL"
     gene            2122..>2688
                     /locus_tag="VGB85_20405"
     CDS             2122..>2688
                     /locus_tag="VGB85_20405"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY0136461.1"
                     /translation="MDDGARSPLATYADLLALPSEERAEVLYGVVHTLPGALPEHNRV
                     QRCLSRFIGGPFDDDDGSGGPGGWWILIETDVRFGPHMITRPDVVGWRRARLESPWGK
                     RPIDVVPDWICEVTSPSNGRRDRVDKSRIYLAQGVPHYWLIDPEERMLEAYSAHDGYW
                     LQTGVYEETSVARIAPFEAIELPVGRLFP"
BASE COUNT          385 a          911 c          924 g          468 t
ORIGIN      
        1 ggcatcggcc ggtaccacgg cgacgagggc ctgctcagct tcatgaaccc caaggcgatc
       61 ctcgtcgacc gcggcaaggc cgactacgac ccgatctggt tcccccacaa ggacaagctg
      121 ggccacgcgc tcaacgtgtt ccgcggggtc gccagcaaga agctgacgag cgtcatcaag
      181 ggcttcctcg gcatgcgcaa ggtcgccgcc gacgcccaca agcccgacgg cgacaagtga
      241 tcgggacggc gcgcggttga catcgcgcgc ggtccatgta gcctcggcgg cgatgaccga
      301 ggttccgggg gcgaagaagg agcgctgggg cacgcgggtc ggcctggtgt tggcggtggc
      361 cggcaacgcg gtcggcctcg ggaacttcct gcgcttcccg gcccaggccg cgcaaaacgg
      421 cggcggcgcg ttcctcatcc cgtacctgat ctcgttcctg ctgatgggcc tgccgctgct
      481 gtgggtcgag tgggcgatcg gccgccacgg cgggcagtac ggccaccact cggcgcccgg
      541 catgttcgcc cgcatgggca agtcgccgtg gctcaagtac gtcggcgtgt tcggcctgtt
      601 caccaacctg acggtcgccg cgtactactg ttacatcgag agctggacgc tggcgtacgt
      661 gtggcactcg ctggccggca cgttcgtgag cacgccggcg accgagttct tcccggacta
      721 cctcgggacc accaacgcgt cgatcacggc gatcccctac gaggccgcgg gctggttcgt
      781 cgccacgctc gcgctcaaca cgtacatcct gtcgcgcggg ctggtgaagg gcgtcgagct
      841 ggcggccaag atcggcatgc cgctgctgct gctgttcggc atggtcctgg cgatccgcgg
      901 cctgaccctc gggccaggcg acccgggcgt gatcgagagc ccgctcgccg gcctcaactt
      961 cgtgtggcaa ccgcagacct cgggcctgtg gaacccgagc acctggctcg ccgccgccgg
     1021 ccaggtgttc ttcacgctgt cggtcggcat ggggtcgatc cactgctacg cggcgtacct
     1081 gcgcgagcgc gacgacatcg ccctcaacgc gaccaccgcc ggcagcgtca acgagttcgt
     1141 cgaggtcatc ctcggcagcg cgatcctcat cccgatcgcc accgcgtacc tcgggctcaa
     1201 cctcgtgcag gcggcgacag ccaccggcag cggcttcgac ctcgcgtttt tgacgctgcc
     1261 gacgctgttc caccagtggg gcgcgttcgg gccgatcgcc tgcgcgatgt ggttcggcct
     1321 gctgttcttc gccggcatca cgtcgtcgct ggcgatgggc cagccggtcg tcgcgttcct
     1381 gcaggacgag ttcgaggtcc gccgcagccc ggccgccgcc gcgttcggca cggcgacgct
     1441 ggtgctcggc ctgatgtgcg tgatcctcta tcccggcggg accttcgccg agttcgactt
     1501 ctggaccggc actttctcgc tgatcatctt cgcgttcgcc gaggtgatca tcttcggctg
     1561 gatcttcggc atcgagcgcg gctgggccga gatccagcgc ggcgcggacc tgcggctgcc
     1621 cgaatttttc aaatacgtca tcaagtacgt gacgcccgcg ttcatcggcg tggtcctgct
     1681 cggggccctg atcaaaccgg tggggtcgtg gggcgacgcg atggggtcgc tgatctccgc
     1741 cggccactgg ccgctcgacc ccgggagcgt gatcggcaag gtgctcctcg tcggcgccac
     1801 cgagagctgg ttcgacgccc agggcaacat caccaaggtc gccgtcgagg acatcacccg
     1861 cgtcgtcctg atcaccgtgt tcgtgctgat cgccctgctc gtgcgcaagg cctggaaggg
     1921 ccgcgtcatc gtgcacgagc ggcaagaagg gaacgtgaag ctgtgaccac cagcgcctgg
     1981 atcatgctca tctcgacctg gctcgtcgtc ggcgggttct cgatttacct ggttgcgagg
     2041 gtgctgacga cgccgcccag agatggggag taacgcggga tccgtctggt cgcgggggcc
     2101 gcgccgggtt atgctgggtg catggacgac ggagcccgca gtccactcgc cacgtacgcc
     2161 gacctgctgg cgctgccctc agaggagcgc gccgaggtgc tgtatggcgt ggtgcacacg
     2221 ctgccggggg cgctgccgga gcacaatcgc gtgcaacggt gtctgtcgcg gttcatcggc
     2281 ggccccttcg acgatgacga tggcagtggc ggccccggtg gctggtggat cctgatcgag
     2341 accgacgttc gcttcgggcc acacatgatc acgcggccgg atgtcgtcgg ttggcgacgc
     2401 gcgcggctcg agtcgccgtg gggcaagcgg ccgatcgatg tcgtgccgga ctggatctgc
     2461 gaggtcacct cgccttccaa tggtcggcga gaccgcgtcg acaagagtcg catctatctc
     2521 gcgcaaggcg tgccgcacta ctggctgatc gaccccgagg agcgcatgct cgaggcctac
     2581 tcggcgcacg acggctactg gctgcagacg ggcgtgtacg aggagaccag cgtcgcccgc
     2641 atcgccccct tcgaggcgat cgagctgccg gtcggtcggc tgtttccg
//
LOCUS       DASWNJ010001209         2918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_161300, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001209 DASWNJ010000000
VERSION     DASWNJ010001209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161300"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2470
                     /locus_tag="VGB85_20410"
     CDS             <1..2470
                     /locus_tag="VGB85_20410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136462.1"
                     /translation="GCGDDGAMGDSTQSGTTSGTITGMTTGEPTTEQPTDGSATVGTM
                     TGATMTGTTPTEATGTETADTGDTTAGLTGDTTATGTDGTDTDPGPCGDCPANFLCKY
                     DTCIPDLGACTTNDDCPGDSYCDKDGQCIPYGVPADIINDPECTKPALPEGVKPIVQC
                     EWSKPPPGDPTEASSLIYTTPMIADLNLDKDPGKLQPSVIVTTFRQEPGLGRLGTLRV
                     FDGRTCEEQMRIGGDDEPDDADRPGYGTQWAIGDLDGDVPQGGHPEIVGLHRLTDPGD
                     DGPESMIAYAIDSSGPEPVLGRLWYGRDCTTQQVIKFGTARACNGPTILDIDDDGVPE
                     VFIDQMVFDNEGCLISPFADFDYGPDLGLFSVVADVDQDDSPDLVRHDRVSSWDANMG
                     QWQVKSWFVANPAQQKAGHIGIADAGHYTDLFGFDITTLPEVVIVAASKPDAGAADTG
                     SVRMQSLTGEVVWGPVEIYRVGNQIGGRGGAPTISDFDGDGQVEFAVAGASYYAVYDP
                     DCAAAAPPQRPGGTCERPMGMENLPNGVLWAQPSKDVSSNMTGGSIFDFDGDGVGEAV
                     YRDECYLRVYNGKTGEVVFSAPATSGTGLEYPVIADVDGDFATEIVVPRAGGGGGCPS
                     PDPLFPGSGPIKADTGFVILRDPMDRWASSRPVWNQHAYSVTHVRDDVTIPKSSEVVA
                     NWLEGTLNNFRQNSRGGFGELLIADLTVELANLGDLCDFAGGSLELQAEICNRGTNPV
                     QDGVAVRFMQTETVDQPVEQATLVCEAVTSKLLLPGECEIVTCMGEIGGEGNVYVDVD
                     PEDKIADCHPANNLGADAFDLCPG"
     gene            2493..>2918
                     /locus_tag="VGB85_20415"
     CDS             2493..>2918
                     /locus_tag="VGB85_20415"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEY0136463.1"
                     /translation="MSLPRCFGVLGDPVAHSRSPAMHAAAFASLGLPHRYLAFHVAPA
                     GLGAAIAGARALGFGGLNLTLPHKQAALAHVDRLTDVARRIGAVNTVLFTGEEVVGDN
                     TDGAGFVAAARELEGPPVEVATVLGAGGAALAVVDAVVHE"
BASE COUNT          464 a          956 c         1086 g          412 t
ORIGIN      
        1 cggatgtggc gacgacgggg cgatggggga cagcacgcag tccggcacga cgtccggcac
       61 gatcacgggg atgacgacgg gcgagccgac gaccgagcag ccgaccgatg gctcggcgac
      121 cgtggggacg atgaccggtg cgacgatgac gggcacgacg ccgaccgagg cgacaggtac
      181 ggagacggcg gacacgggcg acacgacggc gggcctgacc ggcgacacga ccgcgaccgg
      241 gacggacggc accgatacgg accctggccc gtgtggagat tgcccggcga acttcctgtg
      301 caaatacgac acctgcatcc cggacctcgg ggcgtgcacg accaacgacg attgcccggg
      361 ggactcgtat tgcgacaagg atggtcagtg cattccgtat ggtgtgccgg cggacatcat
      421 caacgacccg gagtgcacca agccggcctt gccggagggc gtgaagccga tcgtgcaatg
      481 tgagtggtcg aagccgccgc cgggcgaccc gaccgaggcg tcgagcctga tctacaccac
      541 gccgatgatc gcggacctca atttagacaa ggatccgggc aagctgcagc cctcggtgat
      601 cgtcacgacg tttcgccagg agccggggct cgggcgtctc gggaccctgc gcgtgttcga
      661 cggccgcacc tgcgaggagc agatgcggat cggcggcgac gacgagccgg acgacgccga
      721 ccgcccggga tatggcacgc agtgggcgat cggcgacctc gacggcgacg tgccgcaggg
      781 cgggcacccg gagatcgtcg gcctgcatcg tctgaccgac cccggcgacg acggccccga
      841 gagcatgatc gcgtacgcga tcgacagcag cgggcccgag ccggtgctgg ggcgcctgtg
      901 gtacgggcgc gactgcacga cccagcaggt catcaagttc gggaccgcgc gggcctgcaa
      961 cgggccgacg atcctcgaca tcgacgacga cggcgtgccc gaggtgttca tcgaccagat
     1021 ggtctttgat aatgaggggt gtttgattag tccgttcgcg gacttcgatt atgggcccga
     1081 cctcgggctg ttctcggtgg tcgccgacgt cgaccaggac gattcgcccg acctcgtgcg
     1141 ccacgaccgg gtgtcgagct gggatgcgaa catggggcag tggcaggtca agtcgtggtt
     1201 cgtcgccaac ccggcgcagc agaaggccgg gcacatcggc atcgccgacg ccgggcatta
     1261 cacggacctg ttcggcttcg acatcacgac cttgcccgag gtcgtgatcg tcgccgcgtc
     1321 gaagcccgac gccggcgcag cggacacggg gtcagtgcgc atgcagagcc tgaccggcga
     1381 ggtggtgtgg ggtccggtcg agatctatcg cgtcggtaat cagatcggcg gacgcggcgg
     1441 ggcgccgacg atcagcgact tcgacggcga cggccaggtc gagttcgcgg tcgccggcgc
     1501 cagctactac gcggtctacg accccgactg cgcggccgca gcgccgccgc agcgccccgg
     1561 cggcacctgc gagcggccga tggggatgga gaatcttccc aatggcgtgc tgtgggccca
     1621 gccgtcgaag gatgtctcgt cgaacatgac gggcgggtcg atcttcgact tcgacggcga
     1681 cggggtcggc gaggcggtct accgcgacga gtgttacctg cgcgtgtaca acggcaagac
     1741 cggcgaggtc gtgttcagcg cgccggcgac gagcggcacc ggtctcgagt atccggtgat
     1801 cgccgacgtc gacggcgact ttgcgaccga gatcgtggtg ccgcgcgcgg gtggaggcgg
     1861 cggctgcccg tcgcctgacc cgctgttccc cggctcgggc ccgatcaagg ctgacaccgg
     1921 cttcgtgatc ctgcgcgacc cgatggaccg ctgggcctcg tcgcggccgg tgtggaacca
     1981 gcacgcgtac tcggtgacgc acgtgcgcga cgatgtcacg atcccgaagt cgagcgaggt
     2041 ggtcgccaac tggctcgagg gcacgctcaa caacttccgg cagaacagcc gcggcgggtt
     2101 cggtgagctg ctgatcgccg acctgacggt cgagctggcc aacctcggcg acctgtgcga
     2161 cttcgcgggc ggctcgctcg agctgcaggc ggagatctgc aaccgcggca ccaacccggt
     2221 gcaggacggc gtcgccgtgc gcttcatgca gaccgagacg gtcgatcagc cggtcgagca
     2281 ggcgacgctg gtgtgcgagg cggtgacgag caagctgctg ctgcccggcg agtgcgagat
     2341 cgtgacgtgc atgggcgaga tcggcggcga ggggaacgtc tacgtcgatg tcgacccgga
     2401 ggacaagatc gcggactgcc acccggccaa caacctcggc gccgacgcct tcgacctgtg
     2461 cccgggctga cggcacgcgc taggatgcgg cgatgagcct gccgcgttgc ttcggggtcc
     2521 tcggcgatcc ggtcgcccac tcgcgctcgc cggcgatgca cgcggccgcg ttcgcgtcgc
     2581 tcggcctgcc gcaccgttac ctggcgtttc acgtcgcgcc ggcggggctg ggcgccgcga
     2641 tcgcgggggc gcgggcgctc ggcttcggcg ggctcaacct gaccctgccg cacaagcagg
     2701 ccgcgctcgc gcacgtcgat cgcctgaccg acgtggcgcg gcggatcggc gcggtcaaca
     2761 cggtgctgtt caccggtgag gaggtcgtcg gcgacaacac cgatggcgcc ggcttcgtcg
     2821 ccgcggcgcg cgagctcgag gggccgccgg tcgaggtcgc cacggtgctc ggcgccggcg
     2881 gggccgcgct cgcggtcgtc gacgcggtcg tgcacgag
//
LOCUS       DASWNJ010001210         2143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_553417, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001210 DASWNJ010000000
VERSION     DASWNJ010001210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553417"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="VGB85_20420"
     CDS             <1..980
                     /locus_tag="VGB85_20420"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEY0136464.1"
                     /translation="GDVLVGLAPAAAILALISFMEAFSATLAVARRGEHLDPSQELIA
                     LGLANAAAGLVRGYPIAGGLSRTAVNAQAGARTPLAGVFTAMLVALTLVLLAPLLRTL
                     PRAVLSAIIMVAVAGLIDIGFVRELWRVKRRELLSFAVTFGATLIFGVGLGLAFGVGT
                     SLLFFVLRTTRPHTAVLGRLPGTTVYRNVLRFPDAETVPGLVIVRLDAPLFFANAAYL
                     TAQVRCLVEGKPVRAVILDMGAVYEIDVSALASLRELDRDLSDQNITLHLADLKGPVR
                     DVLGRTDFPAALGAARFTFTVHEAVVRIQDPSPPPCDPRVTQAWCGVQS"
     gene            complement(997..1563)
                     /locus_tag="VGB85_20425"
     CDS             complement(997..1563)
                     /locus_tag="VGB85_20425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY0136465.1"
                     /translation="MSTTPRKVAVFVGSLRAGSFSRMAARALADVAPALELEIVEIGQ
                     LPFYNQDEDGATPPPAYTAFRAQVQAADAVLFVTPEYNRSVPAVLKNAIDVGSRPYGH
                     SVWNGKPGAVMSITPGALGAFGANHHLRQSLVFLNVPTMQQPEAYIAGAATLFDASGA
                     LTNEGTRNFFKTFMESFAAWIELNLRAK"
     gene            complement(1609..>2143)
                     /locus_tag="VGB85_20430"
     CDS             complement(1609..>2143)
                     /locus_tag="VGB85_20430"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY0136466.1"
                     /translation="HLRDGALRLVVVADTHSRPHAQARTHMAAQAPDHILHGGDIGDL
                     EVLDDLARVAPVLAVRGNIDARTPALPDVLTLDIRDGDTSLLTIVVVHIAVAGPKLRA
                     DALRLARSEGASLVVCGHSHVPFIGRDRGVAVFNPGSIGPRRFSLPIVFGVLDITPAN
                     VGMRHVDCETGAVWTPG"
BASE COUNT          283 a          724 c          800 g          336 t
ORIGIN      
        1 acggcgatgt gctcgtcggc ctcgcgcccg cggcggcgat cctggcgttg atctcgttca
       61 tggaggcgtt ctcggcgacg ctggcggtcg cgcgccgcgg cgagcacctc gacccgagcc
      121 aggagctgat cgcgctcggc ctcgccaacg cggcggcggg cctggtgcgc ggctacccga
      181 tcgccggcgg gctgtcgcgc accgccgtca acgcgcaggc gggggcccgc acgccgctgg
      241 cgggggtgtt cacggcgatg ctggtggcgc tgacgctcgt gctgctcgcg ccgctgctgc
      301 gcacgctccc gcgggcggtg ctctcggcga tcatcatggt cgcggtcgcg ggcctgatcg
      361 acatcggctt cgtgcgcgag ctgtggcgcg tcaagcgacg cgagctgctg tcgttcgcgg
      421 tgaccttcgg cgcgacgctg atcttcggcg tcgggctcgg cctggcgttc ggcgtcggca
      481 cctcgctgct gttcttcgtg ctccgcacga cccgcccgca caccgcggtg ctcggtcgac
      541 ttccgggcac gacggtctat cgcaatgtgc tgcgcttccc cgacgccgag acggtgcccg
      601 ggctcgtgat cgtccgcctc gacgcgccgc tgttcttcgc caacgcggcg tacctgacgg
      661 cgcaggtccg ctgcctggtc gagggcaaac cggtgcgcgc ggtgatcctc gacatgggcg
      721 cggtctacga gatcgacgtg tcggcgctgg cgagcctgcg tgagctcgac cgcgacctga
      781 gcgaccagaa catcaccctg cacctggcgg acctcaaggg gcccgtgcgc gacgtgctcg
      841 gtcgcaccga cttccccgcg gccctcggcg cggcgcgctt caccttcacg gtccacgagg
      901 cggtggtcag gatccaggat ccatcgccgc cgccctgcga tccacgggtg acgcaggcgt
      961 ggtgcggcgt gcagtcatga ggttgcagcc ggcgcgtcac ttcgcgcgca ggttgagctc
     1021 gatccaggcg gcgaagcttt ccatgaaagt tttgaaaaaa ttgcgggtgc cctcgttggt
     1081 cagcgcgccg ctggcgtcga agagggtcgc cgcgccggcg atgtaggcct cgggctgctg
     1141 catcgtcggc acgttgagga acacgagcga ctggcggagg tgatgattgg ccccgaaggc
     1201 cccgagcgcg cccggtgtga tgctcatgac ggcgccgggc ttgccgttcc acacgctgtg
     1261 tccgtacggg cgcgagccga cgtcgatcgc gttcttgagc accgcaggca ccgagcggtt
     1321 gtactccggc gtgacgaaca ggacggcgtc ggcggcctgc acctgcgcac gaaaggcggt
     1381 gtacgcgggc ggcggcgtgg cgccgtcctc gtcctgatta taaaacggga gctgcccgat
     1441 ctcgacgatc tcgagctcga gggcgggcgc gacgtcggcg agggccctcg cggccatgcg
     1501 actgaacgat cctgcgcgca ggctgccgac gaatactgcg actttacggg gcgtggtgct
     1561 catgcgcgcc caccctacgc gccggcgcga tcgctgacaa gcggacgctc agcccggggt
     1621 ccacacagcc ccggtctcgc aatccacgtg acgcatgccg acgttggccg gcgtgatgtc
     1681 gaggacgccg aagacgatcg gcaggctgaa gcggcgcggg ccgatcgacc ccggattgaa
     1741 gacggcgacg ccgcggtcgc ggccgatgaa gggcacgtgc gagtggccgc agacgaccag
     1801 cgaggcgccc tcgctgcggg ccaggcggag ggcgtcggcg cgtaacttcg gcccggccac
     1861 ggcgatgtgc acgacgacga tcgtcagcaa ggatgtgtcg ccgtcgcgaa tgtcgagggt
     1921 caacacgtcg ggcagcgcgg gggtcctggc gtcgatgttg ccgcgcaccg cgagcaccgg
     1981 cgcgacgcgg gcgaggtcgt cgagcacctc gaggtcgccg atgtcgccgc cgtggaggat
     2041 gtggtcgggc gcctgcgctg ccatgtgcgt gcgagcctgc gcgtgcggcc tgctgtgcgt
     2101 gtcggcgacg acgacgaggc gcagggcgcc gtcgcggagg tgc
//
LOCUS       DASWNJ010001211         2191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_553504, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001211 DASWNJ010000000
VERSION     DASWNJ010001211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553504"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VGB85_20435"
     CDS             <1..532
                     /locus_tag="VGB85_20435"
                     /inference="COORDINATES: protein motif:HMM:NF019626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine/phenylalanine carboxypeptidase
                     domain-containing protein"
                     /protein_id="HEY0136467.1"
                     /translation="TFAELITNAMDLPRLRRLALRVKAIQMGLDGADFIDLFRFFLAA
                     GQTEEESYFSAARVFRGGDVRGRVVFTKDVVYLQGLISVHTFFLKAIHQGRPEYIRDL
                     FAGRMALGDVLDLDAYFRAGAIAPPRYEPAWIKNQPTLAAFLIYSIFNTRLEIGGLEL
                     ADFHEPQPEPTDDWTG"
     gene            complement(529..837)
                     /locus_tag="VGB85_20440"
     CDS             complement(529..837)
                     /locus_tag="VGB85_20440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136468.1"
                     /translation="MTNLKTTFESGLQELKTLRDEIRVRLHLATQEARGHYESQIEPH
                     VERIEQQLKDAHADTMETVKEALEAAKAAFQEFRGKLGGDQDRQALDETHRAPVKNLS
                     "
     gene            complement(904..>2191)
                     /locus_tag="VGB85_20445"
     CDS             complement(904..>2191)
                     /locus_tag="VGB85_20445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136469.1"
                     /translation="DPSATDPTSTTDTGDTDTGEAGCEPPLPTFVDGKAPTSTLYVKA
                     GAAPGGDGSVAKPFATLLAGITAAQPGTDIRVTGELPGLTYTSGAGGTPEAPIWISGE
                     PGAKIGPLTLESGHDIVLQDLELSSAPEGHVLHFFFADRILIRRLKIHDCGQGCIKGS
                     QATEVYVEDSELWDAGKFKGHPVLDYVGVNGGHIVRSDFHDGPGVMIMLKGGTSDLLF
                     AWNEVHHQTDPGDVLSLGQSTGPQFFQPIDAAFEGLRIIAYANLLHDLTGPPVAFTGC
                     KDCAAVRNTIWNTTGNQLVRFLPGEAGMNSGVMKSIPEGNRFTGNIVVGGQPGGASLN
                     ADEPNTGPNNVLDYNVFLKPGALNWWGVVPQDMVHSTYDQDPMLDAGGIPANVALVDG
                     KGPPDLAGLPFAATFTKDAAGACVQQPPDIGAFDVP"
BASE COUNT          299 a          707 c          799 g          386 t
ORIGIN      
        1 gaccttcgcc gagctgatca ccaacgcgat ggacctgccg cgcctgcgcc ggctcgcgct
       61 gcgcgtcaag gcgatccaga tgggcctcga cggggccgac ttcatcgacc tgtttcgctt
      121 cttcctcgcc gccggtcaga ccgaggagga gagctacttc tccgcggccc gggtgttccg
      181 cggcggcgac gtgcgcgggc gcgtggtctt caccaaggac gtcgtgtacc tgcagggcct
      241 gatcagcgtg cacaccttct tcctgaaggc gatccaccag ggccggcccg agtacatccg
      301 cgacctgttc gccggccgca tggcgctcgg cgacgtgctc gacctcgacg cctacttccg
      361 ggccggcgcg atcgccccgc cgcgctatga accggcgtgg atcaagaacc agccgaccct
      421 ggcggcattt ttgatttact cgatcttcaa tacgcggcta gagatcggtg ggctcgagct
      481 cgcggacttt catgagccgc agcccgagcc gacggatgac tggacgggct agctcaggtt
      541 cttgaccggg gcgcggtggg tctcgtcgag ggcctggcgg tcctggtcgc cgccgagctt
      601 gccgcggaac tcctggaacg cggccttggc cgcctcgagc gcctccttca cggtctccat
      661 ggtgtccgcg tgggcatcct tgagctgctg ctcgatgcgc tcgacgtgcg gctcgatctg
      721 ggactcgtag tggccgcgcg cctcctgggt ggcgaggtgc aggcgcacgc ggatctcgtc
      781 gcgcagggtc ttgagctctt gcaggccgga ctcgaaggtg gtcttgaggt tcgtcatggc
      841 cctggttaat gggtcgagtt gaccgcgacg caagccgcaa accacgcagg gcgcgccggg
      901 cgctcagggc acgtcgaagg cgccgatatc gggcggctgc tgtacgcatg cgccggccgc
      961 gtccttcgta aaagttgcgg cgaagggcag gccggcgagg tcgggcgggc ccttgccgtc
     1021 gacgagcgcg acgttggccg gaatgccgcc ggcgtcgagc atcgggtcct ggtcgtaggt
     1081 cgagtggacc atgtcttgcg ggacgacgcc ccaccagttg agcgcgccgg gctttaaaaa
     1141 gacgttgtag tcgaggacgt tgttcgggcc ggtgttgggc tcgtcggcgt tgagcgaggc
     1201 gccgccaggc tggccgccga cgacgatgtt gccggtgaag cggttgccct cggggatcga
     1261 cttcatcacg ccggaattca tgccggcctc gccgggcagg aagcggacca gctgattccc
     1321 ggtggtgttc cagatcgtgt tgcgcaccgc ggcgcagtcc ttgcaaccgg tgaacgcgac
     1381 cggcgggccg gtgaggtcgt gcagcaggtt ggcgtaggcg atgatccgca ggccctcgaa
     1441 ggcggcgtcg atcggctgga aaaattgcgg gccggtgctc tgcccgagcg agagcacgtc
     1501 gccggggtcg gtctggtggt ggacctcgtt ccacgcgaac agcaggtcgc tggtaccacc
     1561 cttgagcatg atcatcacgc cggggccgtc atggaagtcg ctgcgcacga tgtgcccgcc
     1621 gttgacgccg acgtagtcga gcacggggtg gcccttgaac ttgccggcgt cccacagctc
     1681 ggagtcctcg acgtagacct ccgtcgcctg gctgcccttg atgcagccct ggccgcagtc
     1741 gtggatcttc aggcggcgga tcaggatccg gtcggcgaag aagaagtgca gcacgtggcc
     1801 ctcgggtgcg ctcgacagct cgaggtcctg aaggacgatg tcgtggccgc tctcgagggt
     1861 cagcgggccg atcttcgcgc ccggctcgcc ggagatccag atcggggcct cgggcgtgcc
     1921 gccggcgccg gaggtgtagg tcagcccggg cagctcaccg gtgacgcgaa tgtcggtgcc
     1981 cggctgcgcc gccgtgatgc cggccagcag ggtggcgaag ggcttggcca cgctgccgtc
     2041 cccgcccggg gccgccccgg ccttgacgta cagcgtgctc gtcggcgcct tgccgtcgac
     2101 aaaggtcggc aggggcggct cgcagccggc ctcgccagtg tcggtgtcac cggtgtcggt
     2161 ggtgctggtc gggtcggtcg cgctcgggtc g
//
LOCUS       DASWNJ010001212         3084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_161485, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001212 DASWNJ010000000
VERSION     DASWNJ010001212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161485"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="VGB85_20450"
     CDS             complement(<1..984)
                     /locus_tag="VGB85_20450"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0136470.1"
                     /translation="MAHKGSGVDAEIDRRARGGGPARDAGDVARDPTALDATAGPQLR
                     APAGVTHRPAALDATTAGPQLHAREVVALDVTAPGDLQLHAPASDRRATRADGPLAVD
                     ATTAGNLQLRSHAGAGSEREASLPGEGDRVGRFVVLSELGRGAMGVVFAAYDEELARR
                     VALKLLHFDPQLDASAGRSQLLREAQALARLTHPNVVAVYEAGVWRGSVYVAMEYVQG
                     VDLQAWLAATTRPWREVLTVLRQAGEGLLAAHRSGLVHRDFKPSNVLVGDDGRARVAD
                     FGLAAGRGDAVIAGSTISGPTLLSRTIAGEGALVGTPAYMAPEQIRREEATA"
     gene            1107..2024
                     /gene="prs"
                     /locus_tag="VGB85_20455"
     CDS             1107..2024
                     /gene="prs"
                     /locus_tag="VGB85_20455"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEY0136471.1"
                     /translation="MPRPSHPSLVFALPEYRSMQERLCAAGHEAGEVAIDTFPDGERY
                     MRIVSEVDGRDVVLLGGTTSDAATLMVFDLGCALVRCGARRLTLIVPYYGYATMERAV
                     RAGEVVTAKTRAVMLSAIPMACYGNRILLLDLHAEGIPHYFESGLTAFHVYAKPVLRD
                     VITRLGGGDFVLACTDAGRAKWVESLANDVGVTAAFVFKRRLAGDRTEVSALDAQVQG
                     KRVVIYDDMIRTGGSLLGAARAYRDAGATSLAAVATHGLFPGESLAKIQASGLFTDIV
                     CTDSHPRALALQAQGLVVEPIGQLFLPYL"
     gene            2048..>3084
                     /locus_tag="VGB85_20460"
     CDS             2048..>3084
                     /locus_tag="VGB85_20460"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEY0136472.1"
                     /translation="MQLVKVAAAALNQTPLDWDGNQARIIRAIHDARAQGAALVCLPE
                     LCIPGYGCEDAFHSRGTTLESWRILQELLPETKGIAVALGLPIMHRGALYNAACMIVD
                     GVILGFVAKRFLAGDGIHYEPRWFKPWPPGKHIQWKHDGKAYPLGDLLFEIGNMRVGF
                     EICEDAWVPGRPGPELAAQGVDIILNPSASHFAFGKHEVRKQIVVDASRTFGATYVYT
                     NLIGNEAGRVIYDGEALIASCGKLLAEGPRFGFDDVYITSVVIDIDYARMSHSRTTYL
                     PAVDDSMRAVIHAEFELPPAAYAVPDASQATGWPDARFEKHEEFTRAVTLGLYDYLRK
                     SRSRGFVVSLSG"
BASE COUNT          422 a         1208 c         1017 g          437 t
ORIGIN      
        1 cgccgtcgcc tcctcgcggc ggatctgctc cggtgccatg tacgcgggtg tgccgaccag
       61 cgcgccctcg ccggcgatcg tcctcgatag cagggtcggc cccgagatcg tcgagccggc
      121 gatcaccgcg tcaccgcgac ccgccgccag cccgaagtcg gcgacgcgcg cgcgaccgtc
      181 gtcgccgacg agcacgttgc tcggcttgaa gtcgcggtgc accaggccgc tgcggtgcgc
      241 cgccagcaag ccctcgccgg cctgccgcag caccgtcagc acctcgcgcc acggcctcgt
      301 cgtcgccgcc agccaggcct gcaggtcgac cccctgcacg tactccatcg ccacgtagac
      361 gctgccgcgc cacacccccg cctcgtagac ggcgaccacg ttcgggtgcg tcagccgggc
      421 gagcgcctgg gcctcgcgca gcagctggct gcgcccggcc gaggcgtcga gctgcgggtc
      481 gaagtgcagc agcttgagcg cgacgcgacg cgcgagctcc tcgtcgtacg ccgcgaacac
      541 gacccccatc gcgccgcgac cgagctcgct cagcacgacg aagcgcccga cgcggtcacc
      601 ctcacccggc agcgacgcct cgcgctcgct gccggcgccc gcgtgcgacc gcagctgcaa
      661 gttccccgcg gtcgtcgcgt cgaccgcgag aggaccgtcc gcccgggtcg ctcgccggtc
      721 gctcgccggt gcgtgcagct gcaggtcgcc gggcgcggtg acatcgagcg ccacgacttc
      781 acgcgcgtgc aactgcgggc ccgcggtcgt cgcgtcgagc gccgcgggtc gatgtgtgac
      841 gccggccggc gcgcgcagct gcgggcctgc ggtcgcgtcg agcgcggtgg gatcacgcgc
      901 gacgtcaccg gcgtcacgcg ccggtccgcc gcccctcgcc cgccgatcga tctccgcatc
      961 gacgccgctc cccttgtgtg ccaccaggat cgccacgcta acacaggccc tggcgggccc
     1021 gcggcgcggt cccgcacgat acagcgcggg cgcggcgaac cgcggttgag caactcgcga
     1081 ccgccgggct atgctccccg cccggcatgc ctcgcccctc ccacccgtct ctggtgttcg
     1141 ccctccccga gtaccggagc atgcaggagc ggctgtgcgc cgcgggtcac gaggcgggcg
     1201 aggtggcgat cgacaccttc ccggatggcg agcgctacat gcggatcgtc agcgaggtcg
     1261 acggtcgcga cgtcgtgctg ctcggcggca ccacctccga cgccgcgacg ctgatggtct
     1321 tcgacctcgg atgcgcgctg gtccgctgcg gcgcgcgccg cctgaccctc atcgtcccct
     1381 actacggcta cgcgacgatg gagcgggccg tgcgggccgg cgaggtcgtc accgccaaga
     1441 cccgcgccgt catgctctcg gcgatcccga tggcctgcta cggcaaccgc atcctgctgc
     1501 tcgacctcca cgccgagggc atcccgcact acttcgagag cggcctgacg gcgtttcacg
     1561 tgtatgccaa gccggtcctg cgcgacgtca tcacgcgcct cggcggcggt gacttcgtgc
     1621 tcgcgtgcac cgatgccggg cgcgccaagt gggtcgagtc gctcgccaac gacgtcggcg
     1681 tcaccgcggc cttcgtgttc aagcgccggc tcgcgggcga ccgcaccgag gtctccgcgc
     1741 tcgacgctca ggtgcagggc aagcgcgtcg tcatctacga cgacatgatc cgcaccggcg
     1801 gctcgctgct cggcgccgcg agggcgtacc gggacgccgg cgcgaccagc ctcgccgcgg
     1861 tcgccaccca cggcctgttc cccggcgagt ccctggccaa gatccaggcc tccggtctgt
     1921 tcaccgacat cgtctgcacc gacagccacc cgcgcgcgct cgcgctccag gcccagggcc
     1981 tggtcgtcga gccgattggc cagctgttcc tgccgtacct ctaacctgtc ccgaaggtca
     2041 tctcatcatg cagctcgtca aggtcgccgc cgccgcgctc aaccagaccc cgctcgactg
     2101 ggacggtaat caggcccgca tcatccgtgc gatccacgac gcgcgggccc agggcgccgc
     2161 gctggtgtgc ctgcccgagc tgtgcatccc cggctacggc tgcgaggacg cgttccacag
     2221 ccgcgggacc acgctcgagt cgtggcggat cctgcaagag ctgctgccgg agaccaaagg
     2281 catcgcggtc gccctcggcc tgccgatcat gcaccgcggc gcgctctaca acgccgcgtg
     2341 catgatcgtc gacggcgtga tcctcggctt cgtcgccaag cgctttttgg ccggcgacgg
     2401 catccactac gagccgcggt ggttcaagcc ctggccgccc ggcaagcaca tccagtggaa
     2461 gcacgacggc aaggcctacc cgctgggcga cctcctgttc gagatcggga acatgcgcgt
     2521 cggcttcgag atctgcgagg acgcgtgggt gcccgggcgc cccgggcccg agctcgccgc
     2581 gcagggcgtc gacatcatcc tcaacccgag cgccagccac ttcgcgttcg gcaagcacga
     2641 ggtccgcaag cagatcgtcg tcgacgcgtc gcggaccttc ggcgcgacct acgtctacac
     2701 caacctgatc ggcaacgagg ccggccgcgt catctacgac ggcgaggccc tgatcgcgtc
     2761 gtgcggcaag ctgctcgccg agggcccgcg cttcggcttc gacgacgtgt acatcacctc
     2821 cgtcgtgatc gacatcgact acgcgcgcat gagccactcg cgcacgacct acctccccgc
     2881 cgtcgacgac agcatgcgcg ccgtcatcca cgccgagttc gagctgcccc ccgccgcgta
     2941 cgccgtcccg gacgcgagcc aggcgaccgg gtggcccgac gcccgcttcg agaagcacga
     3001 ggagttcacc cgcgcggtga cgctgggcct ctacgactac ctgcgcaaga gccggtcgcg
     3061 cggcttcgtc gtgtcgctca gcgg
//
LOCUS       DASWNJ010001213         4534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_161505, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001213 DASWNJ010000000
VERSION     DASWNJ010001213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161505"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="VGB85_20465"
     CDS             <1..1032
                     /locus_tag="VGB85_20465"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY0136473.1"
                     /translation="HTFTQPRGELVTLTIESTALRDNLLGDPATRTVLVYLPAGYHDS
                     DARYPMFVDLAAFSGSGLRRVAWTGFGESVPQRIDRLVADGAMGPVIAVFPDCFTSLG
                     GNQYVDSAVMGNWATFLSDELVPRISAEFRVRPGPGGRALYGKSSGGYGALVHGMRRA
                     DLWGAVACHSGDMGFDLVYRPDLPRALDVLARDGGSVPAFLARIAAAKKIGGPDFQAL
                     TMIAMAASYDPRPSEPGRVKLPVDPHTAELDEAAWARWLAHDPLHMLAEPTCQANLRK
                     LRGLYIDVGSRDQYMLHYGARALARRLTTAGIDHFYEEFPDDHNSTDYRLDVSLPFLY
                     RALERGGRA"
     gene            1100..2845
                     /locus_tag="VGB85_20470"
     CDS             1100..2845
                     /locus_tag="VGB85_20470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136474.1"
                     /translation="MRAATRLLAVLAGASLTSCNADPGRRADPDLDNRAVPEPTKEQP
                     PVPVDPEVEAAAKISAKVQRFAPAELQADISGLPESEQQALQKLIAAARLMDPILDRQ
                     SWAGTPALRERLRRDGGPLGVARLEYFELMRGPWDRQDHFAPFAIDKPHPPGAGFYPE
                     DMTADEFKKWVSDHPKDRAAFESLTTMIVRDSIGLRAVPYREAFKEWLEPSAQLLREA
                     AALTKNASLKKFLESRAKAFGTDDYYQSDKDWMDLDSPVEITIGPYETYEDELLGLKA
                     TFEAFVTVSDPKASADLARFKNYLPDMEQHLPIPDDIKTKRGSESPIRVVDLVFTSGD
                     ARKSVQTIAFNLPNDEKVRAEKGAKKVLLRNLIATKFNVIMRPIGEQVMVGEQIRYLD
                     DQAFFQEVLFHELSHSLGPAFTKKDGKDVDVRIALEGSYSPLEEAKADVMGAYNVLYM
                     IERGEFPKDMREKLLVSYFAGLFRSVRFGVAEAHGKGAALQINRLVEAGAAVVDETTG
                     KFKVDLEKIEEAIAGLVRDICVIQHAGDKAAADAMLAKYGVVSPAMQRALDGLGAIPV
                     DIRPFYPLAGEILPK"
     gene            2870..>4534
                     /locus_tag="VGB85_20475"
     CDS             2870..>4534
                     /locus_tag="VGB85_20475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HEY0136475.1"
                     /translation="MSERTAPRPLRELAAGLLAQRSATVSSDALLEVFLDWVTENGLE
                     LYPAQEEAILEVLAGKHVILNTPTGSGKSLVAVAMHLASMARGRRSFYTCPIKALVSE
                     KFFALCRDFGPENVGMLTGDASVNHDAPIICCTAEILANMALRDGRAARVDDIIMDEF
                     HYYSDRERGIAWQIPLLTLPYANFMLMSATLGDTSKLEESLKRLTGREVTVVRSAVRP
                     VPLDFEYRETPLHETLAYLLDRERAPIYIVNFTQRECAEEAQNLMSLQLVSREEKHAI
                     AEALQGFRFDTPFGKEISGFIRNGIGLHHAGLLPKYRLLVEQLAQQGKLRVICGTDTL
                     GVGVNIPIRTVLFTKLCKYDGENTAILSVRDFKQIGGRAGRKGFDTQGYVVCQAPAHV
                     IENKRLEAKSLGADGKKKKFVRRGPPERGYVPWDKATFEKLITSEPEPLRSRFQVTHG
                     MIMNLLQSPLNEGAWGGGYRRLVEIIAAAHDESRAKSRNRREAAVLFRALRTAGIVHL
                     IALNQRKQGSRGRAVRVDADLQHDFSLNHTLSLYLLDASKRLELASE"
BASE COUNT          744 a         1702 c         1416 g          672 t
ORIGIN      
        1 cacacgttca cccagccgcg cggcgagctc gtcaccctca cgatcgagtc gaccgccctg
       61 cgtgacaacc tgctcggcga ccccgcgacg cgcaccgtcc tcgtgtacct ccccgccggc
      121 taccacgaca gcgacgcgcg ctacccgatg ttcgtcgacc tcgccgcgtt ctccggctcc
      181 ggcctgcgcc gcgtcgcctg gaccggcttc ggcgagtccg tcccgcagcg catcgatcgc
      241 ctcgtcgccg acggcgcgat gggccccgtc atcgccgtgt tccccgactg cttcacctcg
      301 ctcgggggca accagtacgt cgactccgcc gtcatgggca actgggcgac cttcctctcc
      361 gacgagctgg tgccgaggat ctccgccgag tttcgcgtgc gccccggtcc gggcgggagg
      421 gccctgtacg gcaagtcgag cggcggctac ggggcgctgg tccacggcat gcgccgcgcc
      481 gacctctggg gcgccgtcgc gtgtcactcc ggcgacatgg gcttcgacct cgtctatcgc
      541 cccgacctac cgcgcgccct cgacgtgctc gcccgcgacg gcggctccgt gccggccttc
      601 ctcgcgcgca tcgccgccgc gaagaagatc ggcggccccg acttccaggc cctgacgatg
      661 atcgccatgg ccgccagcta cgacccacga ccctccgagc ccggccgcgt caagctcccg
      721 gtcgacccgc acaccgcgga gctcgacgag gcggcctggg cccgctggct cgcgcacgac
      781 cccctgcaca tgctcgcgga gccgacctgt caggccaacc tgcgaaagct ccgcggcctc
      841 tacatcgacg tcggctcgcg cgaccagtac atgctgcact acggcgcccg cgcgctggcc
      901 cggcgcctga ccaccgccgg catcgatcat ttttatgagg aattcccgga cgaccacaac
      961 agcaccgatt accggctgga tgtctcgctg ccgtttctgt atcgggcgct cgagcgcggc
     1021 ggccgggcct agaacctgtc ccgagggaca gccgaccgct ggcgccccgc cggcccgttc
     1081 ggttatgctc gcgccccgta tgcgcgccgc aacccgcctc ctcgccgtcc tcgccggtgc
     1141 ctccttgacc tcgtgcaacg ccgaccccgg tcgtcgcgcc gaccccgacc tcgacaaccg
     1201 ggccgtgccg gagcccacga aggagcagcc gcccgtgccg gtcgaccccg aggtggaggc
     1261 cgccgcgaag atctccgcca aggtccagcg gttcgcgccc gcggagctgc aggccgacat
     1321 ctccgggctc ccggagtccg agcagcaggc cctgcaaaag ttgatcgccg ccgcgcgtct
     1381 gatggacccg atcctcgacc ggcagtcatg ggccggcacc ccggcgctgc gtgaacgcct
     1441 gcggcgcgac ggcggcccgc tcggcgtcgc ccggctcgag tacttcgagc tcatgcgcgg
     1501 cccctgggat cgccaggacc acttcgcccc gttcgcgatc gacaagccgc acccgcccgg
     1561 cgccggcttc tacccggagg acatgaccgc ggacgaattt aaaaagtggg tcagcgacca
     1621 ccccaaggac agggccgcct tcgagagcct gaccacgatg atcgtccgcg actcgatcgg
     1681 cctgcgcgcg gtgccctacc gcgaggcttt caaagagtgg ctcgagccct cggcgcagct
     1741 gctgcgcgag gccgccgcgc tgacgaagaa cgcctcgctc aagaaattcc tcgagtcgcg
     1801 cgcgaaggcc ttcggcaccg acgactacta tcagagcgac aaggactgga tggacctcga
     1861 cagccccgtc gagatcacga tcggccccta cgagacctac gaggacgagc tgctcgggct
     1921 caaggcgacc ttcgaggcct tcgtcaccgt cagcgacccg aaggcctccg ccgacctcgc
     1981 ccgcttcaaa aattacctgc cggacatgga gcagcacctc ccgatcccgg acgacatcaa
     2041 gaccaagcgc ggcagcgaga gcccgatccg cgtcgtcgac ctcgtgttca cctcgggtga
     2101 cgcgcgcaag tccgtgcaga cgatcgcctt caacctcccc aacgacgaga aggtccgcgc
     2161 cgagaagggc gccaagaagg tcctgctgcg caacctgatc gccaccaagt tcaacgtcat
     2221 catgcggccg atcggcgagc aggtgatggt cggcgagcag atccgctacc tcgacgacca
     2281 ggcatttttt caagaggtcc tgttccacga gctgtcgcac agcctcggcc ctgccttcac
     2341 caagaaagat ggcaaggacg tcgacgtccg catcgccctc gagggcagct actccccgct
     2401 cgaggaggcc aaggccgacg tgatgggcgc ctacaacgtg ctctacatga tcgagcgcgg
     2461 tgagttcccc aaggacatgc gtgagaagct gctggtctcc tacttcgcgg gcctgttccg
     2521 cagcgtccgc ttcggcgtcg ccgaggccca cggcaagggc gccgcgctgc agatcaaccg
     2581 cctggtcgag gccggcgccg ccgtcgtcga cgagacgacc ggcaagttca aggtcgacct
     2641 cgagaagatc gaggaggcga tcgccgggct cgtgcgtgac atctgcgtga tccagcacgc
     2701 gggcgacaag gccgcggcgg acgcgatgct cgccaagtac ggggtcgtct cccccgccat
     2761 gcagcgcgcg ctcgacggcc tcggcgccat ccccgtcgac atccgcccgt tttacccgct
     2821 cgccggcgag atcctgccga agtagccggt ttgggctacc ctgccgcccg tgtccgaacg
     2881 taccgcgccc cggcccctgc gtgagctcgc cgccgggctc ctggcccaaa ggtcagccac
     2941 agtctcgagc gacgccctgc tcgaggtctt cctcgactgg gtgaccgaga acggcctcga
     3001 gctgtacccc gcgcaggagg aggcgatcct cgaggtgctc gcgggcaagc acgtcatttt
     3061 gaacacgccg acgggctccg gcaagtcgct ggtcgcggtg gcgatgcacc tggcctcgat
     3121 ggcccgcggc cgccgaagtt tttacacctg cccgatcaag gcgctcgtca gcgagaagtt
     3181 cttcgcgctg tgccgcgact tcgggcccga gaacgtcggc atgctgaccg gcgacgcgag
     3241 cgtcaaccac gacgcgccga tcatctgctg caccgcggag atcctcgcca acatggcgct
     3301 acgcgacggc cgggccgcgc gcgtcgacga catcatcatg gacgagtttc attactactc
     3361 ggaccgtgaa cgcggcatcg cctggcagat cccgctgctg acgctgccgt acgcgaactt
     3421 catgctgatg tcggccacgc tcggcgacac cagcaagctc gaggagagcc tcaagcgcct
     3481 gaccggccgc gaggtgaccg tcgtgcgctc cgcggtgcgc ccggtgccgc tcgacttcga
     3541 gtaccgcgag acgccgctgc acgagaccct cgcgtacctg ctcgaccgcg agcgcgcgcc
     3601 gatctacatc gtcaacttca cgcagcgcga gtgcgccgag gaggcgcaga acctgatgtc
     3661 cctccaattg gtctcgcgcg aggagaagca cgcgatcgcc gaggcgctgc agggctttcg
     3721 cttcgacacc ccgtttggaa aagagatctc gggcttcatc cgcaacggca tcggcctgca
     3781 tcacgcgggc ctgctgccga agtatcgctt gctcgtcgag cagctggccc agcagggcaa
     3841 gctgcgcgtc atctgcggca ccgacacgct cggcgtcggc gtcaacatcc cgatccgcac
     3901 ggtcctcttc acgaagctgt gcaagtacga cggcgagaac acggcgatcc tcagcgtccg
     3961 cgacttcaag cagatcggcg gccgcgccgg ccgtaagggc ttcgatacgc agggctacgt
     4021 ggtctgccag gcgcccgcgc acgtgatcga gaacaagcgc ctcgaggcca agtcgctcgg
     4081 cgccgacggc aagaagaaga agttcgtccg ccgcggcccg cccgagcgcg gctacgtccc
     4141 gtgggacaag gccacctttg aaaagctgat caccagcgag cccgaaccac tgcgctcgcg
     4201 cttccaggtc acgcacggca tgatcatgaa cctgctgcag tcgccgctca acgagggcgc
     4261 ctggggcggt ggttatcgcc ggctggtcga gatcatcgcc gcggcgcacg acgagtcgcg
     4321 cgccaagtcc cgcaaccgcc gcgaggccgc ggtgctgttc cgggccctac gtaccgcggg
     4381 catcgtccac ctgatcgcgc tcaatcagcg caagcagggc agccgcggcc gggccgtgcg
     4441 cgtcgacgcc gacctccagc acgacttctc gctcaaccac acgctgtcgc tctacctgct
     4501 cgacgccagc aagcgcctcg agctcgcctc cgag
//
LOCUS       DASWNJ010001214         2472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_480899, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001214 DASWNJ010000000
VERSION     DASWNJ010001214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480899"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VGB85_20480"
     CDS             <1..366
                     /locus_tag="VGB85_20480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136476.1"
                     /translation="SQGGYGGSQNFGGCSQGGYGGGSQGGSQGGYGGSQNYGGSSQGG
                     YGGSQGGHGGSQGGGYGGSQGHIGQGSHGGSQSFGQHGGGSQGSFGSQGGSQGDDGRG
                     GYGGHEQGQHGHEGHRHSR"
     gene            518..955
                     /locus_tag="VGB85_20485"
     CDS             518..955
                     /locus_tag="VGB85_20485"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0136477.1"
                     /translation="MTKNPPEGMQRITPGLVYTDAPAAIEFLRDAFGFEVRERLDMSD
                     GRVGHAQLELEGGVVTLSSVWPELGLASPRDLTAVHGQTRCFVDDVDAHHARARAAGA
                     TIVAGLSDEFYGDRIYRAVDPEGHRWIFATHVRDVPPEEWPAM"
     gene            952..1290
                     /locus_tag="VGB85_20490"
     CDS             952..1290
                     /locus_tag="VGB85_20490"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY0136478.1"
                     /translation="MTGPDLDDTLAALADPTRRRVVELLRDGPRRAGDLASTFELSAP
                     AMSRHLKVLRTSGLIEVTGGDTDARLRIYHLRREPFAALQAWLGEVEAFWGDQLGAFK
                     KHVERKARRP"
     gene            1287..1760
                     /locus_tag="VGB85_20495"
     CDS             1287..1760
                     /locus_tag="VGB85_20495"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY0136479.1"
                     /translation="MTIPSSDRVEVTTLVAVDPETAFTVFTEELDLWWRYGPRYRTQG
                     DRSMLRMEAGVGGRLLEYGGVEPVVLGEVLVWDPARRLVLQWRRGFADGEGTEVEVRF
                     IVEGAGTRVTIEHRGWDRVPEGHRARQGLVGHAFEAATGLWWGDLLVSLRARAKA"
     gene            1785..>2472
                     /locus_tag="VGB85_20500"
     CDS             1785..>2472
                     /locus_tag="VGB85_20500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136480.1"
                     /translation="MQPAGGNRVLEDFLRLAQQRGEASVATARMVACLVFLGMHLPAR
                     VHLLAVGDLKSWILIACLAVGIGFSILGLLWSRAGTVSQLRLDLSIAVDAMVVYVTLG
                     AGVIWPHALYSGVVREPELAAVYLGTIGAGTRLSRRGVLLGAALHGLGLAALLFLDCR
                     NAEILDYSATEMVFVVALFAVAALMGHSVAGRTRALVLQGASAAVLAERARQRLGVYV
                     SEAVASTAMNS"
BASE COUNT          323 a          752 c         1007 g          390 t
ORIGIN      
        1 tcgcagggcg gctacggtgg ctcgcagaac ttcggcggct gctcgcaggg cggctacggt
       61 ggtggctcgc agggcggttc gcagggcggc tacggtggct cgcagaacta cggtggcagc
      121 tcgcagggtg gctacggcgg ctcgcagggt ggccatggcg gctcgcaggg cggcggctac
      181 ggtggctcgc agggccacat cgggcagggc agccatggtg gctcgcagag cttcggccag
      241 cacggtggcg ggtcgcaggg cagcttcggg tcgcagggcg gctcgcaggg tgatgacggc
      301 cgcggtggtt acggcggtca cgagcagggc cagcatggcc acgagggtca ccgccacagc
      361 cgctgatcca gcgtgagcgt cgacgcgcga gcgtcgagga gggcgacgcc gcggagcacc
      421 gcggcgtcgt ttttttgctc gaggcgcgtt gacaatcctt aacggattcg ttaaatgttt
      481 cctgtcccgc gcaagtactt cgggacagga gaacatcatg acgaagaacc ctccagaggg
      541 catgcagcgg atcacaccgg ggctggtgta cacggacgcg ccggcggcga tcgagttctt
      601 gcgcgacgcg ttcggcttcg aggtgcgcga gcgcctcgac atgagcgatg gcagggtcgg
      661 gcacgcgcag ctcgagctcg agggcggcgt ggtcacgctg tcgtcggtgt ggccggagct
      721 cggcctggcg agcccgcggg acctcactgc ggtgcacggg cagacgcgct gcttcgtcga
      781 cgacgtcgac gctcaccacg cgcgcgcgcg tgctgcggga gcgacgatcg tcgccgggct
      841 gtcggatgag ttctatggcg accgcatcta tcgtgcggtc gaccccgagg ggcaccgatg
      901 gatcttcgcc acgcatgtgc gggacgtgcc gcccgaggag tggccggcga tgtgacgggc
      961 ccggacctcg acgatacgct cgcggcgctg gcggacccga cgcggcggcg cgtggtcgag
     1021 ctgctgcgtg acgggccgcg gcgcgcaggc gacctcgcgt cgaccttcga gctcagcgcg
     1081 ccggcgatga gccggcacct caaggtgctg cgcacctcgg ggctgatcga ggtcaccggc
     1141 ggcgacacgg acgcgcgcct gcggatctac cacctgcggc gcgagccgtt cgcggcgctg
     1201 caggcgtggc tcggcgaggt cgaggcgttc tggggcgacc agctcggcgc cttcaaaaag
     1261 catgtcgagc gcaaggcgag gcgaccatga ccattccatc gagtgaccgc gtggaggtga
     1321 cgacgctcgt cgccgtcgat ccggagaccg cgttcaccgt gttcaccgag gagctcgacc
     1381 tgtggtggcg gtatggaccg cggtatcgca cgcagggcga tcgcagcatg ttgcgaatgg
     1441 aggcgggcgt cggcggacgg ctgctcgagt atggcggcgt cgagccggtg gtgctcggcg
     1501 aggtgctcgt gtgggatccg gcgcggcggc tggtattgca gtggcggcgc ggcttcgcgg
     1561 acggcgaggg gaccgaggtc gaggtgcgct ttatcgtcga gggcgcaggc acgcgggtga
     1621 cgatcgagca tcgcggctgg gaccgggtgc cggagggcca tcgcgcgcgg caagggctgg
     1681 tcggacacgc cttcgaggcg gcgaccgggc tgtggtgggg cgacctgctg gtgtcgctgc
     1741 gcgcccgcgc gaaggcgtga tagcctcgct acatcgaggc cgtggtgcag ccggcggggg
     1801 gcaaccgggt gctggaggac ttcctgcgcc tggcccagca gcgcggtgaa gcatcggtgg
     1861 cgacggcccg catggtcgca tgtctggtgt tcctcggcat gcacctgccg gcgcgcgtgc
     1921 acctgctcgc ggtcggcgac ctgaagtcgt ggatcttgat cgcgtgcctg gcggtcggga
     1981 tcgggttctc gatcctcggg ttgctgtgga gccgcgcggg cacggtgtcg cagctgcggc
     2041 tggacctgtc gatcgcggtc gacgcgatgg tcgtctacgt gacgctcggc gcgggcgtga
     2101 tctggccgca cgcgctgtac tccggcgtcg tgcgggagcc cgagctggcc gcggtgtatc
     2161 tggggacgat cggcgcaggc acccggctgt cgcgccgcgg ggtgctgctc ggcgcggcgc
     2221 tgcacgggct cgggctcgcc gcgctgctgt tcctcgactg caggaacgcg gagatcctcg
     2281 attactcggc gacggagatg gtgttcgtgg tcgcgctgtt cgccgtggcg gcgctgatgg
     2341 ggcacagcgt ggcgggcagg acgcgcgcgc tggtgttgca gggcgcgagc gcggcggtgc
     2401 tggccgagcg ggcgcgccag cggctcgggg tgtatgtgtc ggaggcggtc gcgtcgacgg
     2461 cgatgaacag ca
//
LOCUS       DASWNJ010001215         3380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_658384, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001215 DASWNJ010000000
VERSION     DASWNJ010001215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658384"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1632)
                     /locus_tag="VGB85_20505"
     CDS             complement(<1..1632)
                     /locus_tag="VGB85_20505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEY0136481.1"
                     /translation="MPATRRNNHGIRGNNHADALTEQVPPPPWGPGEIRALAAQVRED
                     SERPGAAPWDLLVVGAGITGAGVARDAAMRGLRVLVVDGRDLAYGTSSRSSRLIHGGV
                     RYLEQGEIGLVFEALRERRRLYKIAGHLVSPTRFVFPAYRGDRLPLWKLRVGLSLYDA
                     LSLYRSRGHRTLGPAATRRLEPLLVAEGLRGAVTYEDAVTDDARLTLTTLQSARRHGA
                     EVLTYTPVVGLTREGELHVAELGDGSKVRARSVLVAAGPWTSERLLGTAGRQLLSLSR
                     GIHIVLKAEDLPLRQPVVVQAAKERRILFVVPWGARTYLGTTDTAYEGDPGASGVTSA
                     DAEDLFKLIGRILPSQGLRHDRIVSAWSGVRPLVRAASADDGDSTVELSRSHRIIETA
                     TGVLAVVGGKLTTYRAMAEEAVDRVEKALGRDLPRCTTHRRPLVPGVPLDPAELGNPV
                     LADLAGRHGPLARELAERVARQPALGERLIADLPYLWVEVEHAIAAEGCVHLEDILRR
                     RLPLALTDAELGVRVARPVAERLVAAWGGPASEVDA"
     gene            1668..3359
                     /locus_tag="VGB85_20510"
     CDS             1668..3359
                     /locus_tag="VGB85_20510"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0136482.1"
                     /translation="MSSYTLHIQYVDRPAETRTFTQAKVTLGRDSGDIVLHDTQVSGR
                     HGEITFEGGTLRYTDVGSTNGSFLLQGQRVANIELTPGIALRLGNSLITVQVIDAPNV
                     AGKGRTVIAGPGMAPGFPAPMRPPGASVPPAGPAGIPRPGMPFPAPAPPSPPPGGFAH
                     APTAQMQSPAMPVGMPPGPSGMPGMAPAPGAFIPQPMQPMTMPAPVPGAPLGGPAPMT
                     PVPNPITQPPNPITQPPQPLAQQPMAPPPMAPPGPPPGGYAGFPQAPGYPQAPGGYPP
                     QAPGGYGGVPQGPVATPPASSMSAPAMSSPAMSGNNMVDPVAGAANDVMGFVTGAWEA
                     IKPQIVQATMITAILTVPMAVLGWFVLVTFGPFGFGAVLLILLALAQLVGTLILTPAL
                     VRFVLGVHLGQPIDVRGAIMEQVADLKTVAPNFLVFGLLAGVAAMFLVVPAVFVAVFV
                     GQIYFVEGKRYLDAIGRNFELVKRDAITPILNVFVPALVFGLGLFIAGYILGVLPLIG
                     GLMGALGRAIGQAIMLPVILMLCTRLYFQQRRRFEGGDPEGEARARLGAPALPAA"
BASE COUNT          429 a         1312 c         1167 g          472 t
ORIGIN      
        1 cgcgtcgacc tcgctggccg ggccgcccca cgcggcgacc agacgctcgg cgaccggccg
       61 cgcgacgcgg acgcccagct cggcgtcggt cagcgcgagc ggcaggcggc ggcgcaggat
      121 gtcctcgagg tgcacgcagc cctcggccgc gatcgcgtgc tcgacctcga cccacaggta
      181 cggcaagtcg gcgatcaggc gctcgccgag cgcgggttgc cgggcgacgc gctcggcgag
      241 ctcacgcgcg agcgggccgt gccggccggc gaggtcggcg agcaccgggt tgccgagctc
      301 ggcagggtcg agcggcacgc ccggcaccag cggccggcgg tgggtggtgc agcgcggcaa
      361 gtcgcggccg agcgccttct cgacccggtc gacggcctcc tcggccatcg ccctgtaggt
      421 cgtcagcttg ccgccgacga ccgcgagcac gccggtggcg gtctcgatga tgcggtggct
      481 gcgggacagc tcgacggtcg agtcgccgtc gtcggccgag gcggcgcgca cgagcggccg
      541 gacccccgac cacgcgctga cgatgcggtc gtgccggagc ccctgactcg gcaagatgcg
      601 gccgatcagt ttaaataagt cctccgcgtc ggccgacgtg actccggagg cccccgggtc
      661 gccctcgtag gccgtatcgg tggtgccgag ataggtgcgg gcgccccacg ggaccacgaa
      721 caggatccgc cgctccttgg ccgcctggac gacgaccggc tggcgcagcg gcaggtcctc
      781 ggccttgagc acgatgtgga tcccgcgcga cagcgagagc agttgccggc cggcggtgcc
      841 gagcagccgc tcggaggtcc acggcccggc cgcgacgagc accgagcgcg cccgaacctt
      901 gctgccgtcg ccgagctcgg ccacgtgcag ctcgccctcc cgcgtcagcc cgaccaccgg
      961 cgtgtacgtc agcacctcgg ccccgtgccg gcgcgcgctc tgcagcgtcg tcagggtcag
     1021 gcgcgcgtcg tcggtgaccg cgtcctcgta ggtgaccgcg ccgcgcaggc cctcggcgac
     1081 gagcaagggc tcgaggcggc gggtcgcggc gggcccgagg gttcggtggc cgcggctgcg
     1141 atacaaactg agcgcgtcgt acagcgacag gccgacgcgc agcttccaca gcggcagccg
     1201 gtcgccgcgg tacgccggga acacgaagcg cgtcggcgag accaggtgcc cggcgatctt
     1261 gtacaggcga cgacgctcgc gcaacgcctc gaataccagc ccgatctcgc cttgctcgag
     1321 ataccggacg ccaccgtgga tcagccggct cgagcgggag ctcgtgccgt aggcgaggtc
     1381 ccggccgtcg accacgagca cccgcagccc gcgcatggcg gcgtcgcggg ccacgccggc
     1441 cccggtgatg ccggccccga cgacgagcag gtcccacggt gccgcgccgg gcctctcgct
     1501 gtcctcgcgg acctgggccg cgagagcgcg gatctccccc ggtccccagg gcggcggggg
     1561 gacttgttcg gtgagcgcat cggcgtgatt gttgccgcgg atgccgtgat tgttgcgcct
     1621 agtggcgggc atttgtgaca ctgtgctcag cctacctgga gacctcgatg tcctcgtaca
     1681 cgctccacat ccaatacgtc gaccgccccg ccgagacccg cacctttacc caagccaagg
     1741 tcacgctcgg ccgtgacagc ggcgacatcg tcttgcatga cacccaggtc tcgggacgac
     1801 acggcgagat caccttcgag ggcggcaccc tgcgttacac cgacgtcggc tcgaccaacg
     1861 gcagcttcct gctgcagggc cagcgcgtcg ccaacatcga gctcacgccg ggcatcgcgc
     1921 tccgcctcgg caactcgttg atcacagtgc aggtcatcga cgcccccaat gtagccggaa
     1981 agggccgcac cgtgatcgcc ggtccgggca tggcccccgg ctttcccgcg cccatgcgac
     2041 cccccggtgc ctcggtcccc cccgcaggac ccgccggcat cccgcgcccc ggcatgccgt
     2101 tcccggcgcc ggccccccct tcgcccccgc ccggcggctt cgcccacgcc ccgaccgcgc
     2161 agatgcagag ccccgcgatg cccgtcggca tgccccccgg cccgtccggc atgcctggca
     2221 tggcccccgc gcccggcgct ttcattcctc agccgatgca gccgatgacg atgcccgcgc
     2281 cggtcccggg cgcgccgctc ggtggccccg cgccgatgac cccggtgccg aacccgatca
     2341 cgcagccgcc gaacccgatc acgcagccgc cgcagccgct ggcgcagcag ccgatggccc
     2401 cgccgccgat ggccccgccg ggcccgccgc cgggtggcta cgccgggttc ccgcaggcgc
     2461 cgggctatcc tcaggcgccg ggcggctatc cccctcaggc gccgggtggt tacggcgggg
     2521 tcccgcaggg ccccgtggcg accccgcccg cgtcctcgat gtccgccccc gcgatgtcgt
     2581 caccggcgat gtccggcaac aacatggtcg acccggtcgc cggcgccgcc aacgacgtga
     2641 tgggctttgt caccggcgcg tgggaggcga tcaagcccca gatcgtccag gcgaccatga
     2701 tcaccgcgat cctcaccgtg ccgatggcgg tgctcgggtg gttcgtgctc gtcaccttcg
     2761 ggccgttcgg cttcggcgcc gtgctgctga tactgctcgc gctggcccag ctggtcggca
     2821 ccctgatcct gacgccggcg ctggtccgct tcgtgctcgg cgtccacctc ggccagccca
     2881 tcgacgtccg cggcgcgatc atggagcagg tcgccgacct caagaccgtc gccccgaact
     2941 tcctggtgtt cggcctcctc gccggcgtcg ccgccatgtt cctggtcgtg ccggcggtgt
     3001 ttgtcgccgt gttcgtcggc cagatctact tcgtcgaggg caagcgctac ctcgacgcga
     3061 tcggccgcaa cttcgagctc gtcaagcgcg acgcgatcac cccgatcctc aacgtgttcg
     3121 tgccggcgct ggtgttcggg ctcggcctgt tcatcgccgg ctacatcctc ggcgtgctgc
     3181 ccctgatcgg cggcctcatg ggcgcgctcg gccgcgcgat cgggcaggcg atcatgctgc
     3241 cggtgatcct gatgctgtgc acccgcctgt acttccagca gcgccgccgc ttcgagggcg
     3301 gcgaccccga gggcgaggcc cgcgcccgcc tcggcgcgcc cgcgctgccc gccgcctgag
     3361 cgcaggccac ctgtccggag
//
LOCUS       DASWNJ010001216         3314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_444248, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001216 DASWNJ010000000
VERSION     DASWNJ010001216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444248"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..1524
                     /locus_tag="VGB85_20515"
     CDS             88..1524
                     /locus_tag="VGB85_20515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEY0136483.1"
                     /translation="MDGAPQTALAPFTGAIAVDAQVAGRVLRAALADGGEYADLFFEH
                     RRSLHVSLEDGRVRSVVAGIDAGVGIRVVQGGSVGFAYAESFEPAVMLAAARTAAQVA
                     RARGVRRSTPRTRTPDRAPAALDPLPAVACIEVLRRVDRGARRADPAIVRVEASLSVS
                     RREILIVTSEAAPVHDVQPLLRLGVTAIATRDGRREAGATGGGGRVGLEHFTAHTPER
                     YGQEAARIAVVNLDARPAPAGTLPVVLAPGDAGIWLHEAVGHGLEADFIRKRSSSYSD
                     RVNHQVAPPCVTLLDDPGVPGGRGSLAVDDEGVVPGVHLLVDRGVLRGLLHDRTSART
                     AGLAPTGSGRRQSFRDLPLPRMSNTWLAAGDEDPADIVRSVQRGIYAAGFSGGQVNIA
                     AGDFVFALTEGYLIEDGKITAPLRGVHLIGHGPTALAAVSRVGHDLRMSDGTWTCTKD
                     GQNVPVGVGMPTVKIDALTIGGECGAAR"
     gene            1521..2894
                     /locus_tag="VGB85_20520"
     CDS             1521..2894
                     /locus_tag="VGB85_20520"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEY0136484.1"
                     /translation="MSLPRASQALLAGLERRAAQILGRLAPMVDHAALRLEHGEMLAA
                     SVRDGALEQLSEARSLGLSLRLVRGGRVACVTTTDLQPAALEPFLAEAVALARLADED
                     PHAEPPPPRTLARHWPELDLFDAGIRRLGADRATRLAILAEAAALRADPRITASGGST
                     CSRTVTHALLATSGGFRGPSAGTRVALRAHVIASDADGRRRSGHYWSGGRHLASLEPP
                     EIVGREAAHRALRGLGAAPIATGVYPVVFAPEAAAGIVALVASCLLGDAVVRERSYLA
                     RRLGTAVASPQVTLVDDPLRARGPASRGFDGEGLATRRNVLVRRGELRGFLLDSASAR
                     RLDLRPTASASGGGGLVHPATSNFYMQAGRRGPRALLAGIRRGLYVRATLGHGFDPGS
                     GYFSRGAEGLLIEDGVLTRPVAEVTISRSLDALLRGIEAVANDFELRTAIASPSFRVD
                     AMTVAGT"
     gene            complement(2903..>3314)
                     /locus_tag="VGB85_20525"
     CDS             complement(2903..>3314)
                     /locus_tag="VGB85_20525"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY0136485.1"
                     /translation="DGRRESLRTGASDMTALRLASSKSHASPKIEGVMRALAIPEARN
                     FRMGSVGVKVSTIARGVTDLYVHASRGTKLWDSCAPHAVLAAAGGQMTDVRGEPLVYD
                     PRHLGNDFGLLASHGPHHAEIAAALAPVIGDWFT"
BASE COUNT          371 a         1308 c         1199 g          436 t
ORIGIN      
        1 gtcggtccat ggccgtaaag acaggcgcac ttgtgcaccc gggcggccgc ccgcgccgtc
       61 tctggcgccc tgctacactg cctcccggtg gatggcgcac cgcagacggc cctggccccg
      121 ttcacgggtg cgatcgccgt cgacgcgcag gtcgccggcc gggtgctgcg cgccgcgctg
      181 gccgacgggg gcgagtacgc cgacctgttc ttcgagcacc gtcgctcgct gcacgtgtcg
      241 ctcgaggacg gccgcgtgcg cagcgtcgtc gccggcatcg acgccggcgt cgggatccgc
      301 gtcgtgcagg gtgggtcggt cggcttcgcc tacgcggaga gcttcgagcc cgcggtgatg
      361 ctcgcggcgg cccgtacggc cgcccaggtc gcccgcgccc gcggtgtccg ccgctccacg
      421 ccgcgcacgc ggacgcccga tcgggcgccg gcagctctcg acccgctgcc cgcggtcgcc
      481 tgcatcgagg tcctgcgccg cgtcgatcgt ggagcccgcc gcgccgaccc ggcgatcgtc
      541 cgcgtcgagg cgagcctctc cgtgtcacgg cgcgagatcc tgatcgtgac cagcgaggcc
      601 gcgccggtgc acgacgtcca gccgctgctg cgcctcggcg tcacggcgat cgccacgcgt
      661 gacggccggc gcgaggcggg cgcgacgggt gggggcgggc gcgtcggcct cgagcacttc
      721 accgcgcaca cgccggagcg ctacgggcag gaggccgcgc ggatcgccgt cgtcaacctc
      781 gacgctcgcc ccgcgcccgc cgggaccctg ccggtcgtgc tcgcccccgg cgacgccggc
      841 atctggctgc acgaggcggt cggtcacggg ctcgaggccg acttcatccg caagcgcagc
      901 tcgagctaca gcgatcgcgt caatcaccag gtcgccccgc cgtgtgtcac cctgctcgac
      961 gaccccggcg tgcccggcgg tcgcggcagc ctcgcggtcg acgacgaggg cgttgtgccg
     1021 ggcgtgcacc tgctggtcga tcgcggcgtc ctgcgcggcc tgctgcacga ccgcaccagc
     1081 gcccgcacgg ccggcctggc gccgaccggc tcgggccggc gccagagctt tcgcgacctg
     1141 ccgctgccgc gcatgagcaa cacctggctc gcggctggcg acgaggaccc cgccgacatc
     1201 gtgcgctcgg tgcagcgagg gatctacgcg gccggcttca gcggcggtca ggtcaacatc
     1261 gccgccggcg acttcgtgtt cgcgctgacc gagggctacc tgatcgagga tggcaagatc
     1321 accgccccgc tgcgcggcgt gcatctgatc ggccacggtc cgaccgcgct cgcggccgtc
     1381 agccgggtcg gccacgacct gcgcatgtcc gatggcacgt ggacctgcac caaggacggc
     1441 cagaacgtgc ccgtcggggt cggcatgccg acggtgaaga tcgacgccct gacgatcggt
     1501 ggcgagtgcg gggcggcgcg atgagcctgc cgcgcgcctc gcaggcgctg ctggcgggcc
     1561 tcgagcggcg cgcggcgcag atcctcggcc ggctcgcgcc gatggtcgac cacgccgcgc
     1621 tgcgcctcga gcacggcgag atgctggccg cgagcgtccg cgacggcgcg ctcgagcagc
     1681 tgtccgaggc ccgcagcctc ggcctctcgc tgcgcctggt ccggggtggt cgcgtcgcct
     1741 gcgtcaccac caccgacctg cagccggcgg cgctcgaacc cttcctcgcc gaggccgtcg
     1801 cgctcgcgcg gctcgccgac gaggacccgc acgccgagcc gccgccgccg cgcacgctgg
     1861 cccgtcactg gcccgagctc gacctgttcg acgccggcat ccgccgcctc ggcgctgacc
     1921 gtgcgacgcg cctggcgatc ctcgccgagg ccgcggcgct gcgggccgac ccgcggatca
     1981 ccgcctcggg cggtagcacc tgctcgcgca cggtcaccca cgcgctgctc gccacctccg
     2041 gcggctttcg tgggccgtcc gccggcacgc gtgtcgccct gcgcgcgcac gtgatcgcca
     2101 gcgacgcgga cggccgccgg cgcagcggcc actactggtc cggcggccgt cacctcgcca
     2161 gcctcgagcc gcccgagatc gtcggccgcg aggccgcgca ccgggccctg cgtggcctcg
     2221 gcgccgcacc gatcgcgacg ggcgtatacc cggtggtctt cgcccccgag gcggccgccg
     2281 gcatcgtcgc gctggtcgcc tcgtgtctgc tcggcgacgc cgtcgtccgc gagcgcagct
     2341 acctcgcgcg acgcctcggc accgccgtcg ccagcccgca ggtcaccctc gtcgacgacc
     2401 cgctgcgcgc ccgtggtccg gcctcgcgcg gcttcgacgg cgagggcctg gcgacgcggc
     2461 gcaacgtgct ggtccggcgc ggcgagctgc gcggcttcct gctcgacagc gccagcgcgc
     2521 ggcgtctcga cctgcgcccg accgcctcgg cgagcggcgg cggcggcctc gtgcaccccg
     2581 cgacgagcaa cttctacatg caggccgggc gccgcggccc gcgggcgttg ctcgccggga
     2641 tccgccgcgg cctctacgtg cgcgccacgc tcggtcacgg cttcgacccg ggcagcggat
     2701 acttcagccg cggcgccgag ggtctgttga tcgaggacgg cgtcctgacc cgcccggtcg
     2761 ccgaggtgac gatctcacgc agcctcgacg cgctgctgcg cggcatcgag gccgtcgcca
     2821 acgacttcga gctgcgcacc gcgatcgcct cgccgagctt tcgcgtcgac gcgatgaccg
     2881 tcgccgggac ctgacccgaa gctcaggtga accagtcgcc gatcaccggc gcgagcgcgg
     2941 cggcgatctc ggcgtggtgc gggccgtgcg aggcgagcag cccgaagtcg ttgccgaggt
     3001 ggcgcgggtc gtagaccagc ggctcgccgc gcacgtccgt catctgcccg cccgcggccg
     3061 cgagcacggc atgcggcgcg cacgagtccc acagcttcgt gccgcgcgag gcgtgcacgt
     3121 acaggtcggt cacgccgcgg gcgatcgtcg acaccttcac gccgaccgag cccatgcgaa
     3181 agttgcgggc ctcggggatc gccagcgccc gcatcacgcc ctcgatcttc ggcgacgcgt
     3241 ggctcttgct gctcgccagg cgcagcgccg tcatgtcgct ggcgccggtg cgcaatgatt
     3301 ctcggcggcc gtcg
//
LOCUS       DASWNJ010001217         4386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_233450, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001217 DASWNJ010000000
VERSION     DASWNJ010001217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233450"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..547)
                     /gene="tssB"
                     /locus_tag="VGB85_20530"
     CDS             complement(53..547)
                     /gene="tssB"
                     /locus_tag="VGB85_20530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HEY0136486.1"
                     /translation="MKTNAGSVAPRERVNITYRPATEGAQEDVELPFKILVLGDFTGR
                     PEDTTVEERAPITVDKDNFAKVMAAQKLSVEISVPNAISDDADAGELPVALRFASLAD
                     FRPEGLAEQVPELRRLLELRAALVALKGPIVNVPGFVRKIRELLKDPGQRARLLQELE
                     LEDA"
     gene            732..1229
                     /gene="tssD"
                     /locus_tag="VGB85_20535"
     CDS             732..1229
                     /gene="tssD"
                     /locus_tag="VGB85_20535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HEY0136487.1"
                     /translation="MAETIHLTLKSNGNQIAGESTQHDLGREKTIECLEFKDRVRTAR
                     ELGTRAPVGRRVHEPLEICKRIDSSSPLLAKALCKNEKIEGVFRFYRPSPVGDGTTEQ
                     FFTIEISDGRVDSIERHSASAIDPAAAMTPPLETVRFVYHTIKWIYEPSGAEYVDNWS
                     ASDAA"
     gene            complement(1311..>4386)
                     /locus_tag="VGB85_20540"
     CDS             complement(1311..>4386)
                     /locus_tag="VGB85_20540"
                     /inference="COORDINATES: protein motif:HMM:NF040127.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HEY0136488.1"
                     /translation="GHPVDVVTGNLVFAACDLDLPGALPLRLTRSYSSTWSARDSPFG
                     RGWSHSLDEAIWVEPRHLVYRAEDGRELELPHSDDPETHVPMHRLTLRRLGPDRWQIE
                     DRSGVRRDFAAIAGDPRPGLARLIRRCDRAGHSLHLRYDDRARLVSAHADGDREIHLH
                     YGENDRLTRIDLPDPDGDGYLPHIHYVHDRDDLVEIHDALGQVTRYRHDRHRIVQETF
                     PNGLSFHFEYDGETSDAACVRTWGDGGLLDHRLIYDRPRRTTLVINACNETTTYRADP
                     RGLVTEIVDPRGAVTRFEHDERLQLTATIDALGHVTRHEYDDRGNCTRSLNPDGTAVV
                     TEYHANFNLPVTRIDASGGISRWTYDPQGRVIRHTDPLGRSTIHQHELSSGTHHVEAI
                     VYADGRSELRTHDTAGRLLRHRPPDGSCHSYRYDRRGRLRDRSDAHGRRETREYDLLG
                     RLTRHTLPNGETRLYTHDARGRIIRACDDLGDLRCGYTGLGWLATCGEANAAPCTVER
                     DLEGRVTRVAGPTGTLLRIERDAAGQVRTTVDALGVERRYTRDLLGRVVAIRRPGGKT
                     TRYTRDAVGHITEIHHDDGMHDSFAYRPDGTLQAATRRHTDGQVTTVRREHDALGRVT
                     REFNIINNNNDHAVALEYDLRDRLTRLRSSLGADLRFIYDERGLTRITVPHESWGLLF
                     ERDRDGHEQARHLPGEILSWWQSDSLGRPTAHGIVASHPPQIHRQRRYSWTHTRLDNI
                     EETTRRRTGVAPPTPSVVALRHDPEGRRVAADLSDGTSWKYFWNGAGELSSASTPNTL
                     IKYRHDALGRRIARICDGVETRWLWHGDVPLHSWAGQLGHDPATWVFDPGGFAPLARL
                     TATTRHAFVADHLGTPLAAFDERGQLAWAAELDERGQPCTSRGDPTLCPFRNPGQIAD
                     PDTGLAYNRFREYDPATGQYLSPDPLGLLGGLDTHAYVDDPRTSTDVFGLSTDRPTSL
                     QVYIAEQLTLDFSLADIAPALADVIVRSSVLPTDRKALVAGAFQNPPRGPCD"
BASE COUNT          668 a         1302 c         1690 g          726 t
ORIGIN      
        1 aggagggagg gctgggcggc ggtggtcatg gcgtgcattg cgggtggtcc tttcaggcgt
       61 cctcgagctc gagctcctgg aggagacgcg cgcgctgccc ggggtccttg agcagctcgc
      121 ggatcttgcg gacaaagccg gggacgttga cgatcgggcc cttgagggcg accagcgcgg
      181 cgcgcagctc gagcagccgg cgcagctccg ggacctgctc ggcgaggccc tcggggcgga
      241 agtcggccag cgaggcgaag cgcagcgcca ccggcagctc gccggcgtcg gcgtcgtcgc
      301 tgatcgcgtt ggggaccgag atctcgacgg agagcttctg cgccgccatc accttggcaa
      361 aattgtcctt gtcgacggtg atcggcgcgc gctcctccac ggtggtgtcc tccgggcgac
      421 ccgtgaagtc gccgaggacg aggatcttga acgggagctc gacgtcttcc tgggcaccct
      481 ccgtcgccgg acgataggtg atgttgacgc gctccctcgg ggcgaccgaa ccagcattcg
      541 ttttcatggt ccgtgggtat ggcccgccgg atcggcgctg tccaggatcg tcgatgaaaa
      601 taatgtgcgg agaattggca gggttgctcc ggcgtcagat tgtgtaattg cgcttacaca
      661 atctgcttcc gtgagtccgc gcagcgccta cacccgggcc cgggcgtgcg gcggttgcta
      721 gcttggcgac catggctgaa accatccatt tgaccctcaa atccaacggc aaccagatcg
      781 cgggggagag cacccagcac gacctcggac gcgagaagac gatcgaatgc ctcgagttca
      841 aggaccgggt gcgtacagcg cgtgagctgg ggacgcgggc gccggtcggt cgtcgcgtgc
      901 acgagccgct cgagatctgc aagcgcatcg actcgagctc gccgctgctg gccaaggcgc
      961 tgtgcaaaaa cgagaagatc gagggcgtgt tccgcttcta tcgcccgagc ccggtcggcg
     1021 acggcaccac cgagcagttc ttcacgatcg agatctccga cggccgcgtc gactcgatcg
     1081 agcgccactc cgccagcgcg atcgacccgg ccgccgcgat gacgcccccg ctcgagaccg
     1141 tccgcttcgt ctatcacacg atcaagtgga tctacgagcc gagcggcgcg gagtacgtcg
     1201 ataactggag tgcgtcggac gcggcatgat cgggtgaagc ggtgaacttt taaaaaaagg
     1261 ggccccgccg agcagcgctc ggcggggctc ttttgtcgta catcgagccg tcagtcgcag
     1321 ggcccgcggg gcgggttttg aaatgcgcca gcgacgagcg ccttgcggtc ggtcgggagg
     1381 acggagctcc gcacgatcac gtcggccagg gccggcgcga tgtctgccag ggagaagtcg
     1441 agcgtgagct gctcggcgat gtagacctgg aggctcgtcg gtcgatcggt ggacaggccg
     1501 aagacatccg tgctggtccg cgggtcgtcg acgtaggcat gggtgtcgag gccgccgagc
     1561 agaccgagcg ggtcagggct caggtattgg cccgtggcag ggtcgtactc gcggaagcga
     1621 ttgtacgcga ggccggtgtc cggatcggcg atctggccgg ggttgcggaa cggacagagc
     1681 gtcggatcgc cccggctcgt gcagggttgg ccgcgctcgt cgagctccgc ggcccaggcg
     1741 agctgaccac gctcgtcgaa ggcggccagc ggtgtgccga ggtggtcggc gacgaaggcg
     1801 tggcgggtcg tcgcggtcag acgggccagc ggcgcgaagc cgccggggtc gaacacccag
     1861 gtcgcagggt cgtggccgag ctggccggcc cagctgtgca gcgggacgtc gccatgccat
     1921 aaccaccgcg tctcgacgcc gtcgcaaatg cgggcgatcc gacgaccaag cgcgtcgtgg
     1981 cggtacttta ttaaagtgtt cggggtggat gcgctgctga gctcgccggc gccgttccaa
     2041 aaatatttcc aggatgtccc gtcggacagg tcggcggcga cacgccggcc ctcggggtca
     2101 tggcgcaggg cgacgaccga cggcgtcggc ggggcgacgc ccgtgcgccg gcgtgtggtc
     2161 tcctcgatgt tatcgaggcg agtgtgggtc cagctgtacc ggcgctgacg atggatctgc
     2221 ggcgggtgac tggcgacgat gccgtgcgcg gtcggacgac cgaggctgtc ggattgccac
     2281 catgacagga tctcaccggg caggtggcgg gcctgttcgt gaccatcgcg gtcgcgctcg
     2341 aacaggaggc cccaggactc gtgcggcacg gtgatgcggg tcaggccgcg ctcgtcatat
     2401 ataaagcgta gatcggcgcc cagcgacgag cgcaggcgag tcaggcggtc gcggaggtcg
     2461 tattcgaggg cgactgcgtg gtcattatta ttattaataa tattaaactc tcgcgtgact
     2521 cggcccagcg cgtcgtgctc gcggcggacg gtggtgacct ggccgtcggt gtgtcggcga
     2581 gtcgcggcct ggagggtccc atcagggcga taagcgaagc tgtcgtgcat gccgtcgtcg
     2641 tggtggatct cggtgatgtg gccgacggcg tcgcgggtgt agcgggtcgt ttttccgccg
     2701 ggacgacgga tcgccaccac gcgtccgagc aggtcacggg tgtagcggcg ctcgacgccc
     2761 agagcgtcga cggtggtgcg gacctggccg gcggcgtcgc gctcgatgcg aagcagggtg
     2821 ccggtggggc cggcgacgcg ggtgacgcgg ccctcgaggt cgcgctcgac ggtgcaaggc
     2881 gcggcgttgg cctcgccgca ggtcgcaagc catcccagcc cggtgtagcc gcagcgcagg
     2941 tcgccgaggt cgtcgcaggc gcggatgatg cgaccacggg cgtcgtgggt ataaaggcgg
     3001 gtctcgccgt tcgggagcgt gtggcgggtg aggcggccga ggagatcgta ttcgcgggtc
     3061 tcgcggcggc catgggcatc gctgcggtcg cgcagacggc cgcggcggtc gtacctgtac
     3121 gagtgacacg agccgtcggg tgggcggtgt cgcagcaacc ggcccgccgt gtcatgggtg
     3181 cgcagctcgc tgcggccgtc ggcatagacg atcgcctcga cgtgatgtgt cccggaggac
     3241 agctcgtgtt gatgtatggt cgaacggccg agcgggtcgg tgtggcggat gacacgaccc
     3301 tgtgggtcgt acgtccagcg cgatatacca ccggaggcat cgatgcgcgt cacggggaga
     3361 ttgaagtttg cgtggtactc ggtgacgacg gcggtcccgt cggggttgag cgagcgcgtg
     3421 cagttgccgc ggtcgtcgta ctcgtgacgg gtgacgtgac cgagggcgtc gatggtcgcg
     3481 gtgagctgga gcctctcgtc gtgctcgaag cgggtgacgg cgccgcgcgg gtcgacgatc
     3541 tcggtgacca ggccgcgtgg gtcggcgcgg taggtggtgg tctcgttgca ggcgttgatg
     3601 acgagcgtcg tgcgccgggg tcgatcgtag atcaggcgat gatcgaggag cccgccgtcg
     3661 ccccatgtgc gcacgcaagc ggcgtcgctg gtctcgccgt cgtactcgaa gtggaagctg
     3721 agcccatttg gaaaggtctc ctggacgatg cggtggcggt cgtgacggta gcgtgtgacc
     3781 tgcccgagag cgtcgtggat ctcgaccagg tcgtccctgt cgtggacgta gtgaatatgc
     3841 ggtaggtagc cgtcgccgtc gggatccggg aggtcgatcc gggtcaggcg atcgttctcg
     3901 ccgtagtgca ggtggatctc acggtcgccg tcggcgtgcg ccgacacgag gcgggcgcgg
     3961 tcgtcgtagc gaaggtgcag cgagtgaccg gcgcgatcgc agcgtcgaat caggcgggcg
     4021 aggccggggc gcgggtcgcc ggcgatcgcg gcgaagtcgc gacgaacgcc gctgcggtcc
     4081 tcgatctgcc agcgatccgg gcccaggcga cgcaaggtga ggcgatgcat ggggacgtgg
     4141 gtctccgggt cgtcgctatg cggcagctcg agctcacggc cgtcctctgc gcgatagacg
     4201 aggtggcgcg gctcgaccca gatggcctcg tcgaggctgt gcgaccagcc gcggccgaac
     4261 ggcgagtcgc gggcgctcca cgtcgagctg taactacggg tcagccgcag gggcagggcg
     4321 ccgggcaagt cgaggtcgca ggccgcgaag acgaggttgc cggtgacgac gtcgacgggg
     4381 tgcccg
//
LOCUS       DASWNJ010001218         3952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_199282, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001218 DASWNJ010000000
VERSION     DASWNJ010001218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199282"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VGB85_20545"
     CDS             <1..767
                     /locus_tag="VGB85_20545"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0136489.1"
                     /translation="NKACTTTCKPNICGDGFVYMGVEECDDGNQVDTDSCTKQCKQAV
                     CGDGFKQPGETCDDGNQVNTDLCNTACNPTKCGDAIVQKPNGSNQTEDCDDGNQVNED
                     ACTNVCHKAVCGDGYTQPPEGCDNGPNNANNKTCNVTCQPTSCGDGVVQNPNGNNQAE
                     TCDDNNNIGTDKCNLCKPTFCGDKIIQNPNGANFAEFCDDGNNVNTDTCNACIKTKCG
                     DGIIQKPNGAGQIEACDDGNTINNDGCSNLCQVNPN"
     gene            complement(842..3517)
                     /locus_tag="VGB85_20550"
     CDS             complement(842..3517)
                     /locus_tag="VGB85_20550"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0136490.1"
                     /translation="MSADAPPEESTEAQGRRIGRYVLLGEIGTGAMGVVLAAYDPDLD
                     RKVALKVLRQGAKGGSQGPLRMRREAQAMAKLSHPNVAQVYEVAEAHGRLYLAMEFIP
                     GATLRAWLAKAPRTWHAVLRVYIEAGRGLAAAHAAGILHRDFKPDNAMIDGEGRVRVL
                     DFGLSRAEPSRDAVDTVSSGESLHVTAAGSLIGTPAYMSPEQHRGGQADARSDQFSFC
                     VALWEALHGVRPFAGDTREEIARNAMIGRLSEQGRRGEVPPWVARILERGLKADPDLR
                     WPSMDALLAALAHDPMRTRRRWIAAVGAIAAIAGAGYGLAVYQVAQAQVCSGGADDLI
                     GVWDEARRGEVSRTIAAIDVPYAGRSLAATVAHLDAYAGRWVAAQDGSCEEHRRGLLS
                     SGLFDRRMACLRQRRAQLAATVEVLGQTTRDNVANMLDMATGLQGVETCEDDARLLAG
                     VPLPAEPGRAARVEAERERLARAQALERGGRHGDALAAVTPMIEEAEALGYLPHMAEV
                     HLLAGRLYMHMSQISQASAQLDLALRQGLEGHVDEIAAEAKAVEIFVLAALARRPTEA
                     LANVPLAWSLVRRAGSPPQLAALLHNSAAAAQSELGETARSVVEYEESLALLVKYAPD
                     DPLRWALVNNLALEFVESGQYERGLVLAQDGLAQVEARFDACYPVAGSLRTVLAGSKM
                     AAGHFASARADLEQAVACFGEDYALHTVDAIGGLAEIEYLTGDDAEARRQIERGAALT
                     ARHPEAGITAARLDCISIDLDVRAGQLARARQSLVDAEARADAIAGAESKILFNVHTR
                     RALLAHLEHDDTAALAALEHAVKWLKPGIWLTEQGLYFFTRARVLHGLGRDRQVVVAQ
                     AERAIDAFRVAGPGFGVRVTEVQDWLAAQPELRAP"
     gene            complement(3592..>3952)
                     /locus_tag="VGB85_20555"
     CDS             complement(3592..>3952)
                     /locus_tag="VGB85_20555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136491.1"
                     /translation="DEAGQIIDLRSGAAGVPAPAPLQSAAETVAASLPWSSPTPRPFK
                     HNLDRPAANELGSCRLVRPWVVGPRQALFHATCEHGDAAVLRVGVHEDGTIAGLSLWR
                     VSDVYFGPPVTVPAESA"
BASE COUNT          629 a         1567 c         1262 g          494 t
ORIGIN      
        1 acaacaaggc ctgcacgacc acctgcaagc ccaacatctg cggcgacggc ttcgtctaca
       61 tgggcgtcga ggagtgcgac gacggcaacc aggtcgacac cgacagctgc accaagcagt
      121 gcaagcaggc cgtctgcggc gacggcttca agcagcccgg cgagacctgc gacgacggca
      181 accaggtcaa caccgacctg tgcaacaccg cctgcaaccc gaccaagtgc ggcgacgcga
      241 tcgtccagaa gcccaacggc agcaaccaga ccgaggactg cgacgacggc aaccaggtca
      301 acgaggacgc ctgcaccaac gtgtgccaca aggcggtctg cggcgacggc tacacccagc
      361 cgccggaggg ctgcgacaac ggcccgaaca acgccaacaa caagacctgc aacgtcacct
      421 gccaaccgac cagctgcggc gacggcgtcg tgcagaaccc caacggcaac aaccaggccg
      481 agacctgcga cgacaacaac aacatcggca ccgacaagtg caacctgtgc aagccgacct
      541 tctgcggcga caagatcatc cagaacccca acggtgccaa cttcgccgag ttctgtgacg
      601 acggcaacaa cgtcaacacc gacacgtgca acgcctgcat caagaccaag tgcggcgacg
      661 gcatcatcca aaagcccaac ggcgccggcc agatcgaagc ctgcgacgac ggcaacacga
      721 tcaacaacga cggctgcagc aacctctgcc aggtcaaccc gaactgagcg aaaatccacg
      781 cgcgacgagc cgtccctgcc aagcaggtgc ggctctcgcc gtttaagggc ctcgaagaaa
      841 cctacggcgc gcgcagctcg ggctgcgccg ccagccagtc ctgaacctcg gtgacccgca
      901 cgccgaaacc cgggccggcc acgcggaacg cgtcgatcgc ccgctcggcc tgggccacga
      961 cgacctgccg gtcccgcccc agcccatgca gcacccgcgc gcgcgtgaag aagtacaggc
     1021 cctgctccgt gagccagatg ccgggcttga gccacttcac ggcgtgctcg agcgccgcga
     1081 gcgcggccgt gtcgtcgtgc tcgaggtgcg cgagcagggc gcgccgcgtg tggacgttga
     1141 acagtatctt gctctcggct cctgcgatgg cgtcggcgcg ggcctcggcg tccacgaggc
     1201 tctgccgggc cctggcgagc tgcccggcgc gcacgtcgag gtcgatgctg atgcagtcga
     1261 gccgcgccgc ggtgataccc gcctcggggt gccgggcggt cagcgctgcc ccacgctcga
     1321 tctgccggcg cgcctcggcg tcgtcgccgg tcaaatactc gatctccgcc agccctccga
     1381 tcgcgtcgac ggtgtgcaat gcatagtcct cgccgaagca ggcgaccgcc tgctcgaggt
     1441 ccgcgcgcgc cgacgcgaag tgacccgccg ccatcttgct tccggccagc acggtgcgca
     1501 gcgagccggc caccggatag cacgcgtcga agcgggcctc gacctgcgcc agcccgtcct
     1561 gcgcgagcac cagcccgcgt tcatactgcc ccgactcgac gaactccagc gccaggttgt
     1621 tgaccagcgc ccagcgcagc ggatcgtccg gcgcatactt gacgagcaac gccaggctct
     1681 cctcgtactc gacgacgctg cgcgccgtct cgccgagctc ggactgggcg gccgcggcgc
     1741 tgttgtgcaa cagcgccgcc agctgcggcg gggagccggc gcgccgcacc agcgaccacg
     1801 ccagcggcac gttcgccagc gcctccgtgg ggcgccgggc cagcgccgcc agcacgaaga
     1861 tctcgaccgc cttcgcctcc gccgcgatct cgtcgacgtg cccctcgagc ccctgacgca
     1921 gcgccagatc gagctgcgcc gacgcctgcg agatctggga catgtgcatg tacagcctgc
     1981 ccgccagcag gtgcacctcc gccatgtgcg gcagataccc gagcgcctct gcctcctcga
     2041 tcatcggtgt cacggccgcg agcgcgtccc cgtgtcgtcc gccgcgctcg agcgcctgcg
     2101 ctcgcgccag ccgctcgcgc tccgcctcca ccctcgccgc ccgccccggc tccgccggca
     2161 atggcacgcc cgcgagcagc cgcgcgtcgt cctcgcaggt ctccaccccc tgcagacccg
     2221 tcgccatgtc gagcatgttc gccacgttgt cgcgcgtcgt ctgcccgagc acctcgaccg
     2281 tcgccgccag ctgggcccgt cgctggcgca ggcaggccat gcgccggtcg aacagccccg
     2341 acgacagcag cccgcgccgg tgctcctcgc acgacccgtc ctgcgccgcg acccagcgcc
     2401 ccgcgtacgc gtcgaggtgg gcgaccgtcg ccgccagcga gcgcccggcg tagggcacgt
     2461 cgatcgccgc gatcgtccgc gagacctcgc cgcgccgcgc ctcgtcccac acgccgatca
     2521 ggtcgtccgc tccgccgctg cacacctgcg cctgcgcgac ctgatacacc gcgagcccgt
     2581 agccggcccc ggcgatcgcc gcgatcgccc ccaccgctgc gatccagcgc ctgcgcgtgc
     2641 gcatcgggtc gtgcgccagc gcggccagca gcgcgtccat cgacggccag cgcagatcgg
     2701 ggtccgcctt gaggccgcgc tcgaggatcc gcgcgaccca cggaggcacc tcgccgcggc
     2761 gcccttgctc gctcagccgg ccgatcatcg cgttgcgcgc gatctcctcg cgagtgtcgc
     2821 cggcgaacgg acgcaccccg tgcagcgcct cccacagcgc cacgcagaag ctgaattgat
     2881 cgctgcgggc gtcggcctgc ccgccgcggt gctgctccgg ggacatgtac gccggcgtcc
     2941 cgatcaggct gcccgccgcg gtcacgtgca gcgattcgcc gctgctgacc gtgtccaccg
     3001 cgtcgcgcga cggttcggcg cgcgacagcc cgaagtccag cacgcgcacg cggccctcgc
     3061 cgtcgatcat cgcgttgtct ggcttgaagt cgcggtgcaa gatccccgcg gcgtgggccg
     3121 ccgccagccc gcggccggcc tcgatgtaca cgcgcagcac cgcgtgccac gtgcgtggcg
     3181 ccttggccag ccaggcccgc agcgtcgcgc ccgggatgaa ctccatcgcc aggtacagcc
     3241 ggccgtgcgc ctcggcgacc tcgtacacct gcgcgacgtt cgggtgcgac agcttggcca
     3301 tcgcctgcgc ctcgcgtcgc atgcgaagcg gcccctgcga cccgcccttc gcgccctgac
     3361 gcaggacctt gagggccacc ttgcggtcga ggtcggggtc ataggccgcg agcacgacgc
     3421 ccatcgcccc ggtcccgatc tccccgagca gcacgtagcg cccgatcctg cggccctggg
     3481 cctcggtcga ctcctccgga ggcgcatcag ccgacatcgc ggcaccgacg ttatcccgaa
     3541 cgcagcgccc gcgacacgtg gcgaccgcga gcgagttcga ccggcgagcg cctacgcgct
     3601 ctccgctggc accgtcactg gcgggccgaa gtacacgtcc gagacgcgcc acagactcag
     3661 cccggcgatc gtgccgtcct cgtgaacccc gacgcgcagc accgccgcgt cgccgtgctc
     3721 acatgtcgca tggaacagcg cctgacgcgg cccgaccacc cacggccgca cgagtcggca
     3781 cgagccgagc tcgttcgccg cgggccggtc gaggttgtgc ttgaacggac gcggcgtcgg
     3841 ggagctccac ggcagcgacg cggccacggt ctccgccgcg ctctgcagcg gcgccggggc
     3901 ggggacgccg gccgcgccgg atcgcaggtc gatgatctgc cccgcctcgt cg
//
LOCUS       DASWNJ010001219         3022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_305579, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001219 DASWNJ010000000
VERSION     DASWNJ010001219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305579"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1279
                     /locus_tag="VGB85_20560"
     CDS             110..1279
                     /locus_tag="VGB85_20560"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0136492.1"
                     /translation="MSTRVELRRDAGRVGPHDGPWVRRRQVAKVWGPADPAALAELTD
                     HRGQTLAWGLLSPDSEIPLRIVGFGAAAPPADWLDRRMTAAVTSRAALGLRDGGPTSG
                     WRVVNSEGDGLPGLTVDRYGEDTVVQVATAAMAARQAEVVRFFAAHTAGRVFVVSPTQ
                     AAEREGFTPQQQLHGDDATLTFTEHGLRFTVPAPPAQKTGAYLDQRDNRRTIAALAQT
                     SGGPLLDVGCHVGGFALHAAALGVPAVGLDQSAKALACAQANAQDNGLAQRCAWVQGD
                     MFAPLEDPALAGPFGVIVFDPPRVASSARDVGRAAGAMTGSLARLLPRVQPGGFVVVC
                     SCSQHLGGEVLDGVMLDAGAGAWTRVHTLGPGPDHPVWPGHREGSYLRVNVYQRR"
     gene            1322..2203
                     /locus_tag="VGB85_20565"
     CDS             1322..2203
                     /locus_tag="VGB85_20565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136493.1"
                     /translation="MPKLPHEALVQLVRSAPEFVFRLLQHELGVDLPLHVPGRVTAAE
                     FVDLNFAEHRADAILTVGDPERPSEAFVVEAQTDINPRKRRSWPIYVAGPHARLGCPV
                     TLVVIAPDPAVAAWAATPIDLGRGRTIVRPLVIGPDQIPVITDHDEARRFPELAVLSV
                     AAHGREPGAEHIALAALVASRDLDSERDIFYADFVFALLGEVARKALEQLMPARNYEF
                     QSDFARKYFYGGKAEGRAEGKAEGKAELLLRQMRRKGFTVDATIEARVADCKDDQLDT
                     WADRVLTATTLDEIFAA"
     gene            complement(2215..2451)
                     /locus_tag="VGB85_20570"
     CDS             complement(2215..2451)
                     /locus_tag="VGB85_20570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136494.1"
                     /translation="MATAVGKAYEGFLISEPVVGRGMVIFRDPNGRRMVTTAVRRILT
                     TEQRRLFYVETENSVYRIAIVDQLAARADSTAVG"
     gene            complement(2663..>3022)
                     /locus_tag="VGB85_20575"
     CDS             complement(2663..>3022)
                     /locus_tag="VGB85_20575"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEY0136495.1"
                     /translation="GGRAPGGVVGSVVGSVVGVLAAIYLVVGGLHGYAVRAESEAPQA
                     IALPAKLEVREGPGNHRKALFVVQGGSRVRIVDRGSGWLRIRLPDGLEGWAPQASLGE
                     LGGEVPRPGSLVRGRTP"
BASE COUNT          438 a         1142 c         1035 g          407 t
ORIGIN      
        1 ccgcgcggtc cggccaggaa cacggccgcg gccagcgctg catgggacac gagcatcgcg
       61 ggcaagatag caaagcgcga cgccggccgg gcgtggtaga tgcgagcgcg tgagcacacg
      121 cgtcgagctt cgccgcgacg cgggccgggt cggcccgcac gatggtccct gggtgcgccg
      181 ccgccaggtc gccaaggtct ggggtcccgc cgatcccgcg gcgctggccg agctgaccga
      241 ccaccgcgga caaaccctcg cgtggggcct gctgtcgccc gactcggaga tcccgctgcg
      301 catcgtcggc ttcggcgccg ccgcgccacc cgccgactgg ctcgatcggc gcatgaccgc
      361 ggcggtcacc agccgggcgg cgctcggcct gcgtgacggg ggaccgacga gcggctggcg
      421 ggtcgtcaac agcgagggcg acggcctgcc cggcctgacg gtcgaccgct acggcgagga
      481 cacggtggtc caggtcgcga ccgcggcgat ggccgcgagg caggccgagg tcgtgcggtt
      541 cttcgcggcg cacacggccg gccgcgtgtt cgtggtctcg ccgacccagg ccgccgagcg
      601 cgagggcttc acgccgcaac agcagctgca cggcgacgac gcgacgctga cgttcaccga
      661 gcacggcctg cgcttcaccg tgccggcacc accggcgcaa aagaccggcg cgtacctcga
      721 ccagcgggat aatcgccgca cgatcgccgc gctcgcgcag accagcggcg ggccgctgct
      781 cgacgtcggc tgtcacgtcg gcggcttcgc gctgcacgcc gcggcgctcg gcgtgccggc
      841 cgtcggcctc gaccagagcg cgaaggccct cgcctgcgcg caggccaacg cgcaggacaa
      901 cggcctcgcc cagcgctgcg cctgggtcca gggcgacatg ttcgcgccgc tcgaggaccc
      961 cgccctcgcc ggcccgttcg gcgtgatcgt gttcgacccg ccgcgcgtcg ccagcagcgc
     1021 ccgcgacgtc ggccgcgcgg ccggggcgat gaccggcagc ctcgcccgcc tgctgccgcg
     1081 ggtccagccg ggcggcttcg tggtcgtgtg cagctgcagc cagcacctcg gcggcgaggt
     1141 gctcgacggc gtgatgctcg acgccggtgc aggtgcatgg acccgcgtgc acacgctcgg
     1201 ccccgggccc gaccaccccg tgtggccggg ccaccgcgag ggctcatacc tgcgggtgaa
     1261 cgtgtatcag cggcgctgag gtgcaacacg ggcgggctcg cggccgaatg ggcatccgtc
     1321 gatgccgaag ctgccgcacg aagccctggt ccaactcgtg cgcagcgcgc ccgagttcgt
     1381 cttccgcctg ttgcagcacg agctcggcgt cgatctgccg ctgcacgtgc ccgggcgcgt
     1441 caccgcggcc gagttcgtcg acctcaactt cgcggagcac cgcgccgacg cgatcctcac
     1501 cgtcggcgac ccggagcgcc cgagcgaggc cttcgtcgtc gaggcgcaga ccgacatcaa
     1561 cccgcgcaag cgccgatcgt ggccgatcta cgtcgccggc ccgcacgcgc gcctcggctg
     1621 cccggtgacg ctcgtcgtca tcgcccccga cccggcggtc gccgcgtggg ccgccacgcc
     1681 gatcgacctc ggccgcggtc gcacgatcgt ccggccgctg gtgatcggac ccgatcaaat
     1741 ccccgtgatc accgatcacg acgaggcccg acgcttcccc gagctcgcgg tgctctcggt
     1801 cgccgcacac ggccgtgaac ctggcgccga acacatcgcg ctcgctgccc tcgtcgccag
     1861 ccgcgacctc gacagcgaac gcgacatctt ttatgctgac ttcgtcttcg ccctgctcgg
     1921 cgaggtcgcc cgcaaggccc tggagcagct catgccagcc cgtaactacg agttccagag
     1981 tgacttcgcc aggaagtatt tttacggcgg caaggccgaa ggcagggccg agggcaaggc
     2041 ggagggcaag gccgagctgc tgctgcgaca aatgcggcgc aagggcttca ccgtcgacgc
     2101 caccatcgag gcgcgcgtgg ccgactgcaa ggacgaccag ctcgatacct gggcggaccg
     2161 cgtgctgact gccaccaccc tcgacgagat cttcgcggcc tgacgcttct gctgtcagcc
     2221 gaccgcggtg ctgtcggcgc gggcggccag ctgatcgacg atggcgatcc gataaacgct
     2281 gttctcggtc tcgacgtaga acagccggcg ctgctcggtg gtcaggatcc gtcggaccgc
     2341 ggtggtgacc atgcggcggc cgttcgggtc gcggaagatg accatgccgc gaccgaccac
     2401 gggctccgag atcaggaagc cctcgtaggc cttgccgacc gcagtcgcca cagacgcctc
     2461 accattgctc cgaatcttgg ttgcgaggac cgcaagcacg attccactac cttatccccc
     2521 ggcggaaaaa acgctacgac caatttcgac cgcaccgggc tccacgcccg tctaccgccc
     2581 gctgggcgat ccctgctgct cccttgcgct agcgagcttc gcgtccacga agtgtttcgg
     2641 cggtgtcgca ttcctggcgc gatcagggtg tccggccgcg gacaagtgag ccgggtcgcg
     2701 ggacttctcc gccgagctcg ccgaggctgg cctgcggtgc ccagccttcg aggccgtccg
     2761 gcaggcggat gcgcagccag cccgagccgc gatcgacgat acgcacccta ctccccccct
     2821 gtacgacgaa cagcgccttg cggtgattgc cgggaccctc gcgcacctcg agcttggccg
     2881 gcagcgcgat cgcctgcggg gcctccgact cggcgcgcac cgcgtagccg tgcagaccgc
     2941 cgacgaccag gtagatcgcc gcgagcacgc cgaccacgct accgaccacg ctgccgacca
     3001 caccaccggg ggcgcggccc cc
//
LOCUS       DASWNJ010001220         3003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_658408, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001220 DASWNJ010000000
VERSION     DASWNJ010001220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658408"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VGB85_20580"
     CDS             <1..322
                     /locus_tag="VGB85_20580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136496.1"
                     /translation="GDEMSDEDWQDRSTRTLGMFLAGKGIDDLDDDGDPIHDDDLLLL
                     LSSDKDPVEFKLPDFRTGPQGWEVLIDTNEDDTWGRRIEGMVPMAARSLLLLRRVNDT
                     EAAT"
     gene            319..2136
                     /gene="treZ"
                     /locus_tag="VGB85_20585"
     CDS             319..2136
                     /gene="treZ"
                     /locus_tag="VGB85_20585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEY0136497.1"
                     /translation="MNPETIQPPPVEIRELGATIVDDGVRFRVWSTRAERVAVVLYDK
                     PGDGGEPQIAHEHPLEARGDGLFAATLELTEGALYKFRVNDDLYPDPYARVLPYGVHG
                     PAEVMRPGTGKPIKRERPALSECVIYELHIGTFTPEGTYAAAMKRLPALAELGVTAIE
                     LLPLSSFSGRWGWGYDGVAHFAPHAAYGRPEDLRALVDAAHGLGLAVIFDVVYNHFGP
                     EGNYLGAYSPEYFTDAVKTPWGAALDYATSAMRRFVIENAMYWLTEFDGDGLRLDATH
                     TIHDVSDRKILAELVDAVRGLDDPRILIAEDETNDPQLIVGTGLDAVWADDFHHIVHV
                     LSTGERDGYYDEYEPSLAQLARCIDRGFFYEGQPWRPTGHPRGAPADAMPAPAFVYCL
                     QNHDQVGNRALGERLHHIAPRDLYCAMTALLLFLPTTPMLWMGQEWGASTPWQFFTDH
                     PEELGKLVTEGRRGEFKAFAAFADPNTRAKIPDPQAESTYRNSQLRWEEREQSPHREL
                     LALHRALLQLRRDDAVLRVPDRHRTSVGTDGLRSLWVRRWTDAGQRLLVIDLGERPDL
                     APPAAVHGPNWKLLLHAGAESIPGLPAARAAIYAAEATS"
     gene            2133..>3003
                     /locus_tag="VGB85_20590"
     CDS             2133..>3003
                     /locus_tag="VGB85_20590"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY0136498.1"
                     /translation="MTIPSSTYRLQLHAGFGFAAAAEITDYLHALGVDAVYTSPYLHA
                     ERGSTHGYNIVDHGHFGPELGTEEDYVRWLDDVRARGMGHILDMVPNHMGIGSGENRW
                     WNDVLENGMASLYADNFDIEWDPPKIGMRGKILWPVLGGQFGEELEAGRLKVVREGGA
                     FFVAYYERRLPVTVKSLPHVLRPALERLELAAGDPSRDELHSILTSIDHLHGESSTPV
                     EAREERSREKDVIKRRIDRLLAESPEVTAAVDASLTTLAGEVGKPRSFDALEAMLHDQ
                     VYRLSFWRVATEEI"
BASE COUNT          465 a         1123 c         1012 g          403 t
ORIGIN      
        1 cggcgacgag atgagcgacg aggactggca ggaccgctcg acgcgcacgc tcgggatgtt
       61 cctcgccggc aagggcatcg acgacctcga cgacgacggc gacccgatcc acgacgacga
      121 cctgctgctg ctgctcagca gcgacaagga ccccgtcgag ttcaagctcc ccgactttcg
      181 caccggaccc cagggctggg aggtgctgat cgacaccaac gaggacgaca cgtggggccg
      241 gcgcatcgag ggcatggtgc cgatggcggc ccgctcgctg ctgctgctgc gccgcgtcaa
      301 cgatacggag gccgcgacat gaaccccgag accatccagc ccccgccggt cgagatccgc
      361 gagctcggcg cgacgatcgt cgacgacggc gtccgcttcc gcgtgtggag cacccgcgcc
      421 gagcgcgtcg cggtcgtgct ctacgataaa cccggcgacg gcggcgagcc gcagatcgcc
      481 cacgagcacc cgctcgaggc ccgcggcgac ggcctgttcg ccgcgaccct cgagctgacc
      541 gagggcgccc tctataaatt tcgcgtcaac gacgacctct accccgaccc ctacgcgcgc
      601 gtcctgccgt acggcgtgca cgggccggcc gaggtcatgc gaccgggcac cggaaagccc
      661 atcaagcgcg agcgcccggc gctgtccgag tgcgtgatct acgagctgca catcggcacc
      721 ttcacgcccg agggcaccta cgcggcggcg atgaagcgcc tgcccgcgct cgccgagctc
      781 ggcgtcaccg cgatcgagct gctgccgctg tcgagcttct cgggccgctg gggctggggc
      841 tacgacggcg tcgcccactt cgcgccgcac gccgcctacg gccggccgga ggatctgcgc
      901 gccctggtcg acgcggccca cggtctcggc ctcgcggtga tcttcgacgt cgtctacaac
      961 cacttcgggc ccgaggggaa ctacctcggc gcctacagcc cggagtactt caccgacgcg
     1021 gtcaagaccc cgtggggtgc ggcgctcgac tacgcgacct cggcgatgcg ccgcttcgtg
     1081 atcgagaacg cgatgtactg gttgaccgag ttcgacggcg acggcctgcg cctcgacgcg
     1141 acgcacacga tccacgacgt ctcggatcgc aagatcctcg ccgagctggt cgacgccgtg
     1201 cgcggcctcg acgacccgcg gatcctgatc gccgaggacg agaccaacga cccccagctg
     1261 atcgtcggca ccgggctcga cgccgtgtgg gcggacgact tccaccacat cgtccacgtg
     1321 ctctcgaccg gcgagcgcga cggctactac gatgagtacg agccctcgct cgcgcagctg
     1381 gcccgctgca tcgaccgcgg cttcttctac gagggccagc cgtggcgccc gaccggccac
     1441 ccgcgcggcg cgccggccga cgccatgccc gcgccggcct tcgtgtactg cctgcagaac
     1501 cacgaccagg tcggcaaccg ggcgctgggc gagcggctgc atcacatcgc gccgcgcgac
     1561 ctctactgcg cgatgacggc gctgctgctg ttcttgccga ccacgccgat gctgtggatg
     1621 ggccaggagt ggggcgcgag cacgccgtgg cagttcttca ccgaccaccc ggaggagctc
     1681 ggcaagctgg tgaccgaggg tcggcgcggc gagttcaagg ccttcgccgc cttcgccgac
     1741 ccgaacacgc gcgcgaagat ccccgacccg caggccgagt cgacctaccg taactcgcag
     1801 ctgcgctggg aggagcgcga gcagtcgccg caccgcgagc tgctcgcgct gcaccgcgcg
     1861 ctgctgcagc tgcgccgcga cgacgcggtg ctgcgcgtgc ccgaccggca ccgcaccagc
     1921 gtcggcaccg atgggctgcg ctcgctgtgg gtgcgccgct ggaccgacgc cggccagcgc
     1981 ctgctggtga tcgacctcgg cgagcgaccc gacctggcgc cgccggccgc ggtccacggg
     2041 cccaactgga aactgctgct tcacgccggc gccgagtcga tccccggtct gccggccgcg
     2101 cgtgcggcga tctacgccgc ggaggccacc tcgtgacgat cccaagctcg acctatcgcc
     2161 tccagctcca cgccggcttc ggcttcgccg cggccgcgga gatcaccgat tacctgcacg
     2221 cgctcggcgt cgacgcggtc tacacctcgc cgtacctgca cgccgagcgc ggcagcaccc
     2281 acggctacaa catcgtcgac cacggtcact tcgggcccga gctcgggacc gaggaggact
     2341 acgtccgctg gctcgacgac gtgcgcgcgc gcggcatggg tcacatcctc gacatggtgc
     2401 ccaaccacat gggcatcggc agcggcgaga accgctggtg gaacgacgtg ctcgagaacg
     2461 gcatggcctc gctgtacgcc gacaacttcg acatcgagtg ggacccgccc aagatcggca
     2521 tgcgcggcaa gatcttgtgg ccggtgctcg gcgggcagtt cggcgaggag ctcgaggccg
     2581 gccggctcaa ggtcgtccgc gagggtgggg ccttcttcgt cgcctattac gagcgccgcc
     2641 tgccggtcac cgtcaagagc ctgccgcacg tgctccgccc cgcgctcgag cgcctcgagc
     2701 tggccgcggg cgacccctcg cgcgacgagc tgcacagcat cctgacctcg atcgaccacc
     2761 tgcacggcga gtcgagcacg ccggtcgagg cccgcgagga gcggtcgcgc gagaaggacg
     2821 tcatcaagcg gcgcatcgac cgactgctcg cggagagccc cgaggtcacg gccgcggtcg
     2881 acgcgagcct gaccacgctc gcgggcgagg tcggcaagcc gcgcagcttc gacgcgctcg
     2941 aggcgatgct gcacgaccag gtctaccgcc tcagcttttg gcgcgtggcg accgaggaga
     3001 tca
//
LOCUS       DASWNJ010001221         1749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_233537, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001221 DASWNJ010000000
VERSION     DASWNJ010001221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233537"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..>1749)
                     /locus_tag="VGB85_20595"
     CDS             complement(2..>1749)
                     /locus_tag="VGB85_20595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEY0136499.1"
                     /translation="DSASWLFHSTLAYVALRLLGAAPTDPLAVGAMRWIRAHGGVVQI
                     PTWGRIWLAMLGMYPWAGVQPILPEMWLLPEATPVHPRRLYNHMRLIYLGLSYVYGVR
                     LQSAPAPILTAIRDELYPEGYARVPFAAHRDEIAATDLYEMPGAGLRAAFAGMRAIER
                     FTPGLLRKRALARALGHIHFEFRSTDYVCLSPVNGLLFCLALFHSNPRHPELPRALRG
                     LEYWVWEDEAEGMRICGARSDIWDTSFLLQALSEGPALPTAKAIARDAAAWLPRAQIH
                     GDIPGGAEHYREPADGGWGFADEHHPWPVSDCTAEALEALLSLEGHLTEETALSLDRQ
                     RAAVRFILRRQNDDGGFGSYEPRRGSMVLRHFNPAEIYGNCMLEYSYTECTASCVRGL
                     AVARKHMGAALGDVLADQVDDAVRRGAAFLLAAQHKNGAWSGFWGINFTYGTFFAIPA
                     LLASGLSPHHPAIGRAVQWLLGAQRVDGGWGESFQGVVEDRDIALPPEEPSLAVQTAW
                     AVLALMAVSRDDARVRRAVDRGVAYLLARQGTDGAWPEERASGVFFNTAVLDYRLYRQ
                     VFPAWALARYLGRSA"
BASE COUNT          266 a          627 c          629 g          227 t
ORIGIN      
        1 ttcacgccga acggccgagg taccgcgcga gcgcccaggc cgggaatact tgccgataca
       61 ggcgatagtc gagcacggcc gtgttgaaga acacgccgct cgcccgctcc tccggccacg
      121 cgccgtcggt tccctgtcgt gccagcaagt acgcgacgcc gcgatcgacg gcccgccgca
      181 cccgtgcgtc gtcgcggctg accgccatca acgcgagcac ggcccacgcc gtctgcaccg
      241 cgaggctcgg ctcctccggc ggcaacgcga tgtcacgatc ctcgacgacg ccctggaagc
      301 tctcgcccca gccgccgtcg acgcgctggg ccccgagcag ccactgcacc gcgcgaccga
      361 tcgccgggtg gtgcggcgac aggccggagg cgagcagcgc cgggatcgcg aagaaggtcc
      421 cgtaagtgaa gttgatcccc caaaagccgg accacgcgcc gttcttgtgc tgggccgcga
      481 gcaggaacgc ggcgcctctg cgcaccgcgt cgtcgacctg gtccgcgagc acgtcgccga
      541 gcgccgcgcc catgtgcttg cgcgcgaccg ccaggccgcg cacgcagctc gccgtgcact
      601 cggtgtacga gtactcgagc atgcagttgc cgtagatctc ggccgggttg aagtggcgca
      661 gaaccatgct gccgcgccgc ggctcgtacg acccgaagcc gccgtcgtcg ttctggcggc
      721 gcaggatgaa gcggaccgcg gcgcgctggc ggtcgagcga cagggctgtc tcttcggtca
      781 ggtgaccctc gagcgagagc agcgcctcga gggcctcggc cgtgcagtcg ctgaccggcc
      841 acgggtgatg ctcgtcggcg aagccccagc cgccgtcggc cggctcgcgg tagtgttcgg
      901 cgccgccggg gatgtcgccg tggatctgcg cgcgaggcag ccacgcggcg gcgtcgcggg
      961 cgatcgcctt cgcggtcggc agggccgggc cctcggacag cgcctgcagc aggaagctgg
     1021 tgtcccagat gtcgctgcgg gccccgcaga tccgcatgcc ctcggcctcg tcctcccaca
     1081 cccagtactc gaggccccgc agcgcgcgcg gcagctcggg atgtcgggga ttactgtgga
     1141 acagcgccag gcagaacagt aggccgttga cggggctcag gcacacataa tcggtgctgc
     1201 gaaactcgaa gtggatgtgc ccgagcgcgc gggcgagcgc ccgcttgcgc agcagccccg
     1261 gcgtgaagcg ttcgatcgcg cgcatgcccg cgaacgcggc gcgcaggccg gcgccgggca
     1321 tttcataaag atcggtcgcg gcgatctcgt cgcggtgcgc cgcgaacggc acacgcgcgt
     1381 agccctcggg gtacagctcg tcgcggatcg ccgtcaggat cggcgccggc gcgctctgca
     1441 gtcgcacgcc gtacacgtac gagagcccga ggtagatcag gcgcatgtga ttgtagaggc
     1501 ggcgcgggtg caccggggtc gcctcgggca gcagccacat ctcgggcagg atcggctgca
     1561 cgccggccca cgggtacatg ccgagcatcg ccagccagat ccggccccag gtcgggatct
     1621 ggaccacgcc gccgtgggcc cggatccagc gcatcgcccc gaccgcgagc gggtccgtcg
     1681 gcgccgcgcc gagcaggcgc agggcgacgt acgcgagcgt ggagtggaac agccacgaag
     1741 cgctatcgg
//
LOCUS       DASWNJ010001222         3539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_553606, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001222 DASWNJ010000000
VERSION     DASWNJ010001222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553606"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VGB85_20600"
     CDS             <1..571
                     /locus_tag="VGB85_20600"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY0136500.1"
                     /translation="IIFYAGVRRRDTDLLRSLHAALADSDLARAQSLVKNSAAPGGRI
                     ATQMLETSARGHGSMAAIMAALRPGEKLRLERNLNFLGTVGANAPFIGLLGTVLGIIE
                     TFRAIERTGVAGSSEYSNQVMAGIYEALVATAVGLLVAIPAVIAYNIFQRRVKALLSE
                     ADALTNMVLALFSNTTSRPSGPGGNDGRA"
     gene            584..1033
                     /locus_tag="VGB85_20605"
     CDS             584..1033
                     /locus_tag="VGB85_20605"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY0136501.1"
                     /translation="MAGDTNLDDDEGINGINVTPLVDVMLVLLIIFMVTTTKIQESEA
                     MAVDKPDANTGQKLETNKHQILLVCAKDGSITVDLKPVTDDAAIKTAITAKLAEDRDL
                     QGVIKCDEEANVGSMVHLIDILRESGVKKYAIATEKPKSKPQTSPGT"
     gene            1056..1907
                     /locus_tag="VGB85_20610"
     CDS             1056..1907
                     /locus_tag="VGB85_20610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY0136502.1"
                     /translation="MSSGSPYARPSLGAAGSIVVHVVVAALLAYFGQDECATQQTACL
                     AAGNAPLMCQAVYDVCKGAPPTYEITEFELPPEPEPEPPKPEPEPEPEPEPIVKPVVP
                     PSKVATPKPERASKPAPISDKPKSDEPPPPFRMDQSQTVQNGNPVKVNVGTPGGVPGG
                     TGDPKKNGTGSRPSNAPVGDGDAAPWEPRGELYIRELPQVLSVPQEQCPAVQELGIEG
                     VVILTVQVRRDGTVKEVKISKDIGHGCGKVAAKALRRAKFKPAVATNGQPADFELRYE
                     YEFQLNE"
     gene            1930..>3539
                     /locus_tag="VGB85_20615"
     CDS             1930..>3539
                     /locus_tag="VGB85_20615"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY0136503.1"
                     /translation="MSLRTTIAAALLALLAARPAVAQAPEGYEAPVLQAAVDLDYPAS
                     LRSLPDPPAGRIELKYVVGVDGVPREIEVTKALHPVLDAAARDAVSRLRYQPGQLRGR
                     PVEVVLALGLDIAPPEPLPAAPTSPVPADSPAAEPASPVPKDSPPASAPERLRGRVLS
                     AGQRTPVPGASVLVLPAPADEPLGVLKKTRYGEDPPPAWTMRAVTDVDGRFSLRGVPS
                     GKLRVIVLAQGFGRLEAVERLGDNERIELAYYPEPLVFNPYKTVVKSRRDDSGEVDRR
                     TITPEEISKLPGTQGDALKAIQNFPGVARAPFGAGLLVIRGAAPSDSATFLGYHEIPQ
                     LFHFGGLTSVFNADILTQIDFVPGNFDSRFGDAIGGIINVSPRKGRRDGFHGYVKSDV
                     FDTGILAEGPIGKGSLIVAGRRSYIDALLPLAIPRDAGLNFTLAPRYYDYQLLFDYPI
                     SGGEFSARVFGSDDRSKLLFSAANDTDPSNNAGLETTQFFHRADLVYRKTLGPWEFLV
                     TPSYRRDNISFLIGDAFRFLVITDVFSGR"
BASE COUNT          587 a         1387 c         1080 g          485 t
ORIGIN      
        1 catcatcttt tacgctggcg tgcggcgccg cgacaccgac ctgctgcgct ccctccacgc
       61 cgcgctcgcc gacagcgacc tcgcccgcgc ccagtcgctc gtcaagaaca gcgccgcccc
      121 cggcggtcgc atcgccaccc agatgctcga gaccagcgca cgcggtcacg gctcgatggc
      181 agcgatcatg gcggccctgc gccccggcga gaagctgcgc ctcgagcgta acctcaactt
      241 cctcggcacc gtcggcgcca acgccccgtt catcgggctg ctcggcaccg tgctcggcat
      301 catcgagacc ttccgcgcca tcgagcgcac cggcgtcgcc ggctcgtcgg agtactcgaa
      361 ccaggtcatg gccggcatct acgaggcgct cgtcgccacc gccgtcggcc tgctggtcgc
      421 gatcccggcc gtcatcgcct acaacatctt ccagcgccgc gtgaaggcgc tgctgagcga
      481 ggccgacgcg ctgaccaaca tggtcctggc tctcttcagc aacaccactt cacggccctc
      541 cgggcctggt gggaacgacg ggcgcgcgta acaggggcgg acagtggcgg gcgacaccaa
      601 cctcgacgac gacgaaggca tcaacggcat caacgtcacc ccgctggtgg acgtgatgct
      661 cgtgctcctg atcatcttca tggtcacgac cacgaagatc caggagtccg aggcgatggc
      721 ggtcgacaag ccggacgcca acaccggtca aaagctcgag accaacaagc accagatcct
      781 gctcgtctgc gccaaggacg gttcgatcac ggtcgacctc aagccggtga ccgacgacgc
      841 cgcgatcaag accgcgatca ccgcgaagct cgccgaggac cgcgacctgc aaggcgtgat
      901 caagtgcgac gaagaggcca acgtcggctc gatggtgcac ctcatcgaca tcttgcgtga
      961 gtcgggcgtg aagaagtacg cgatcgccac cgagaagccg aagtcgaagc cccagaccag
     1021 ccccggaacc tgaagcgttg acggagccag cgcccttgag ctccgggagc ccatacgcgc
     1081 ggcccagcct cggcgccgcg ggatctatcg tcgtccacgt cgtggtcgcg gcgctgctcg
     1141 cctactttgg tcaggacgag tgcgcgaccc aacagaccgc atgcctcgcc gctggcaacg
     1201 ccccgctcat gtgccaggcc gtctacgacg tgtgcaaggg cgcccccccg acctacgaga
     1261 tcaccgagtt cgagctgccc cccgagcccg aacccgaacc acccaagccc gagcccgagc
     1321 ccgaaccgga gcccgagccg atcgtgaagc ccgtggtccc gccgagcaag gtcgcgactc
     1381 cgaagcccga gcgcgcctcc aagcccgcgc cgatctccga caagcccaag tccgacgagc
     1441 cgccgccgcc gtttcgcatg gaccagagcc agaccgtgca aaacggcaac ccggtcaagg
     1501 tcaacgtcgg tacccccggt ggtgtgcccg gtggcaccgg cgacccgaag aagaacggta
     1561 ctggtagtcg cccgagcaac gccccggtcg gggacggcga cgcggccccg tgggagccac
     1621 gcggcgagct gtacatccgc gagctgccgc aggtgctcag cgtgccgcag gagcagtgcc
     1681 cggccgtgca ggagctcggc atcgagggcg tcgtcatcct caccgtgcag gtccgccgcg
     1741 acggcacggt caaggaggtg aagatctcca aggacatcgg ccatggctgc ggcaaggtcg
     1801 ccgcaaaggc cctgcggcgc gccaagttca agccagccgt cgccaccaac ggccagcccg
     1861 cggacttcga gctgcgctac gagtacgaat tccagctcaa cgagtgagct gctcgcctgc
     1921 cccgcgctca tgtccctccg gacgaccatc gccgctgccc tcctggccct gctcgccgcc
     1981 cgccccgcgg tcgcgcaggc gcccgagggc tacgaggcgc ccgtcctgca ggccgcggtc
     2041 gacctcgact accccgcgag cctacgcagc ctgcccgatc cgcccgcggg ccgcatcgag
     2101 ctcaagtatg tcgtcggcgt cgacggcgtg ccgcgcgaga tcgaggtcac gaaggccctg
     2161 cacccggtcc tcgacgcggc cgcccgcgac gccgtcagcc gcctgcgcta ccagccggga
     2221 cagctgcgcg gccgccccgt cgaggtcgtg ctcgccctcg gcctcgacat cgccccgccc
     2281 gaaccactcc ccgcggcccc cacgtcacct gtccccgcgg acagccccgc ggccgagccc
     2341 gcatcacctg tccccaagga cagcccgccc gccagcgccc ccgagcgcct gcgcggccgg
     2401 gtcctctccg ccggccagcg caccccggtc ccgggcgcaa gcgtgctcgt gttgcccgcg
     2461 cccgccgacg agccgctcgg cgtcttgaag aagacccgct acggcgagga cccgccgccc
     2521 gcgtggacca tgcgcgccgt caccgacgtc gacggtcgct tcagcctgcg cggcgtgccg
     2581 agcggcaagc tgcgggtcat cgtcctcgcc cagggcttcg ggcgcctcga ggccgtcgag
     2641 cgcctcggcg acaacgagcg catcgagctc gcctactacc ccgagcccct cgttttcaac
     2701 ccctacaaga cggtcgtcaa gagccgccgc gacgacagcg gcgaggtcga ccgccgcacg
     2761 atcacccccg aggagatcag caagctcccg ggcacgcagg gcgacgcgct caaggcgatc
     2821 cagaacttcc ccggggtcgc gcgtgcgccc tttggcgccg gcctgctcgt gatccgcggc
     2881 gcggcgccca gcgactccgc gaccttcctc ggctaccacg agatccccca gctgtttcac
     2941 ttcggcggcc tgaccagcgt cttcaacgcc gacatcttga cgcagatcga cttcgtgccc
     3001 ggcaacttcg acagccgctt cggcgacgcg atcggcggca tcatcaacgt ctcgccgcgc
     3061 aagggccggc gcgacggctt tcacggctac gtcaagagcg acgtgttcga caccggcatc
     3121 ctcgccgagg gcccgatcgg aaagggctcg ctgatcgtcg ccggtcggcg cagctacatt
     3181 gacgccctgc tcccgctggc gatccccagg gacgccggcc tcaacttcac gctcgccccg
     3241 cgctactacg actaccagct gctgttcgac tacccgatct ccggcggtga gttctcggcg
     3301 cgcgtgttcg gctccgacga ccgaagtaag ctgctgttct ccgccgccaa cgacaccgac
     3361 ccgagcaaca acgccggcct cgagaccacc cagttcttcc accgcgccga cctcgtctac
     3421 cgcaagacgc tcggcccgtg ggagttcctg gtcacgccct cgtaccgccg cgacaacatc
     3481 tccttcctga tcggcgacgc gtttcgcttc ctcgtgatca ccgacgtctt ctcgggtcg
//
LOCUS       DASWNJ010001223         3424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_270833, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001223 DASWNJ010000000
VERSION     DASWNJ010001223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270833"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(332..859)
                     /locus_tag="VGB85_20620"
     CDS             complement(332..859)
                     /locus_tag="VGB85_20620"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY0136504.1"
                     /translation="MATTPAKIQVFTYKDGLLARLAHDLRLSVGRFEVVREGDEVRGK
                     FWPASLAVDGVVGRDGAVDTTALSDSDRKKITGNIAEHVLALARYPEATFTGKIVEGE
                     RAIDGTLTLVGRAVPLRVEVHAANGRLTAEVPLVPSRWGITPYRALAGAIKLQDRVLV
                     KIDLPADGDGRWSAG"
     gene            complement(873..1964)
                     /locus_tag="VGB85_20625"
     CDS             complement(873..1964)
                     /locus_tag="VGB85_20625"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY0136505.1"
                     /translation="MEIIDLPIVQEIYDAILRALPRSLRGYLDPALVVLTAALFGVLI
                     RVTLFRWLRWLTARNRSRYDDIIVTSLTRRIVGWTILGALYFQCEDLPWRPRATLLAE
                     NIVASLLIASVTLVLVRMISSLIQTYGTTSAAGVGGTTLIRYLAAVVLLFVGTVSILA
                     LFGISVVPAITALGIGGLAVALAFQDTLANVFSGLNLTLAGQVRVGDYVKVGTDVDGF
                     VVDIGWRATTLRTIAGLVIFIPNKKLAEAVMTNYTREPGMSIELLFRVEIACDPERVE
                     SIVIDEVQQAAEEMPGIRTDVPAIVRFKAFGEYALEFAAYVDILNFQDRMALRHELMK
                     RLYHRLKREKIAVPVPLLRIDAAAATSSS"
     gene            complement(2000..2926)
                     /locus_tag="VGB85_20630"
     CDS             complement(2000..2926)
                     /locus_tag="VGB85_20630"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEY0136506.1"
                     /translation="MSEVLPVDVDPRLLMVLLAPWAPLLAFVFGALWGSFANVVIYRV
                     PRDLSVVRPGSACPGCGAPIAWYDNIPVLSYLLVRGRCRRCGEKIALRYLVVELLAGV
                     MAFALYMQHVVRPLVQGGGPALAAWLLWLIFGLALLIVTYTDIDLWVIPDEVVLPVAA
                     IGLLTAAIDPTVLGVGVVEAAAAGAVGYLLVAGLRWVYLRFRGLEALGLGDGKLLLMI
                     GAFCGARGLIWALAAGAIQGLIVAVPALLLGKKIATTDLQEVHGDDPELGEDAEGVMG
                     ARVPFGPFLAIAAFEYILIPDQIEALIALVLP"
     gene            2989..>3424
                     /locus_tag="VGB85_20635"
     CDS             2989..>3424
                     /locus_tag="VGB85_20635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136507.1"
                     /translation="MTRPRSEPRTTSLAPRVAILALALTSPLTVLALATLAQACGGEP
                     ATDAAAPAEPAKPGAIDTQAGAIAHAGEQRAAQARGRVDALLAEPTPATLLAVLAQGH
                     AEARALLGPHNLRYKASFALTPEIATRPVVDEPIQQEQRVVDE"
BASE COUNT          560 a         1299 c         1142 g          423 t
ORIGIN      
        1 aagtcgaggc cggcaccgag gcctgcgcgc aggcctccgg gtgcaccttg atctgcgtgc
       61 cgcgctcgag gtcgtggagg atgaagcacc cggggtggcg caccgcggcc gcggcggtcg
      121 ggcgcgacgc gcaggcgagc ccggcgcagg cgaggacgag aggaagcaga cgcataccgg
      181 cccctacgcg gcggcgcgcg ccttgcttcc cgcgccttcg atctcccggg ctgcctggcc
      241 tgcgcaagcg cgacgcaagc agcgccccga tcgccccgca gcggggccct ggcagcctcg
      301 gcgtgcacct gtccctttga ccagacgcgc gtcagcccgc cgaccagcgt ccatcaccgt
      361 ccgccggcag gtcgatcttc acgagcacgc ggtcctgcag cttgatcgcc ccggcgagcg
      421 cccgataagg cgtgatgccc cagcggctcg gcaccagcgg aacctcggcc gtcagccgtc
      481 cgttcgcggc atgtacctcg acccgcaagg gcaccgcgcg gccgacgagc gtcagcgtgc
      541 cgtcgatcgc ccgctcgccc tcgacgatct tcccggtgaa cgtcgcctcg gggtaccgcg
      601 cgagcgccag cacgtgctcg gcgatgttgc cggtgatctt cttgcgatcc gagtccgaga
      661 gcgccgtcgt gtcgaccgcg ccgtcgcgcc cgaccacgcc gtcgaccgcc agcgaggcgg
      721 gccagaactt gccgcggacc tcgtcgccct cgcgcacgac ctcgaagcgg ccgaccgaca
      781 gccggagatc gtgggcgagg cgggccagta gcccatcctt gtacgtgaac acctggatct
      841 tcgcgggtgt ggtcgccatc gtcgtctccg tgtcagctcg atgaggtcgc cgcggccgcg
      901 tcgatccgca gcagcggcac cggcaccgcg atcttctcgc gcttgaggcg gtggtacagc
      961 cgcttcatca gctcatggcg cagggccatg cgatcttgaa agttgaggat gtcgacgtac
     1021 gcggcgaact cgagcgcata ctcgccgaac gccttgaagc gcacgatcgc cggcacgtcc
     1081 gtgcggatcc ccggcatctc ctccgcggcc tgctggacct cgtcgatcac gatcgactcg
     1141 acgcgctcgg ggtcgcacgc gatctcgacg cggaacaaca gctcgatcga catgccgggc
     1201 tcgcgcgtgt agttcgtcat caccgcctcg gcgagcttct tgttggggat gaagatcacc
     1261 agccccgcga tcgtgcgcaa ggtcgtcgcg cgccagccga tgtcgacgac gaagccgtcg
     1321 acgtccgtcc cgaccttgac gtagtcgccg acccggacct gccccgcgag cgtcaggttg
     1381 agcccggaga acacgttggc gagcgtgtcc tgaaacgcca gcgcgaccgc gaggccgccg
     1441 atgcccagcg ccgtgatcgc cggcaccacc gagatcccga acagcgccaa aatcgacacc
     1501 gtgccgacga acagcagcac gaccgcggcg aggtagcgga tcagcgtggt gccgccgacg
     1561 ccggccgcgc tggtcgtgcc gtaggtctgg atcagcgacg agatcatgcg caccagcacc
     1621 agcgtcaccg acgcgatcag cagcgaggcg acgatgttct ccgcgagcag cgtcgcgcgc
     1681 ggccgccacg gcaggtcctc gcactgaaag tacagcgcgc cgaggatcgt ccagccgacg
     1741 atccggcggg tcaggctggt gacgatgatg tcgtcgtagc ggctacggtt gcgcgcggtc
     1801 agccagcgca gccagcgaaa cagcgtgacg cggatcagca ccccgaacag cgccgcggtg
     1861 agcacgacga gcgcggggtc gaggtagccc cgcagcgacc gtggcagcgc ccgcaagatc
     1921 gcgtcgtaaa tttcctgcac gatcgggagg tcgatgatct ccatggcgtc cccgcggccg
     1981 ccgacatacc acgcctcgct tacggcagca cgagggcgat cagcgcctcg atctggtccg
     2041 ggatcaggat gtactcgaag gccgcgatcg cgaggaacgg cccgaacggc acccgcgcgc
     2101 ccatgacgcc ctccgcatcc tcaccgagct cggggtcgtc gccgtgaacc tcctgcaggt
     2161 ccgtcgtggc gatcttcttg ccgagcagca gcgccggcac ggcgacgatc aggccctgga
     2221 tcgcgcccgc ggccagcgcc cagatcagcc cgcgcgcccc gcaaaatgcc ccgatcatca
     2281 gcagcaactt gccgtcgccg aggccgagcg cctcgagccc gcgaaagcgc aggtacaccc
     2341 agcgcagccc cgcgaccagc aggtacccga cggcccccgc cgcggccgcc tcgacgacgc
     2401 cgacgccgag caccgtcggg tcgatcgccg ccgtcagaag gcctatcgcc gcgaccggca
     2461 gcacgacctc gtcggggatc acccacaggt cgatgtccgt gtacgtgacg atcaagagcg
     2521 cgaggccgaa gatcagccac agcagccacg ccgccagcgc gggcccaccc ccctgcacca
     2581 gcggacggac cacgtgctgc atgtacagcg cgaaggccat cacgcccgcg agcagctcga
     2641 cgacgaggta gcgcagggcg atcttctcgc cgcagcggcg gcagcggccg cgcaccagca
     2701 ggtacgagag caccgggatg ttgtcgtacc aggcgatcgg cgcgccgcac cccgggcacg
     2761 ccgagcccgg gcgcaccacg ctgaggtcgc gaggcacgcg atagatcacg acgttggcga
     2821 agctgcccca cagcgcaccg aacacgaagg ccagcagggg cgcccagggg gccagcagca
     2881 ccatcaggag ccgcggatcg acgtcgacgg gcaggacttc cgacaaggcc cgcactgtag
     2941 ctcgccgccg acgcgcgcgc acgcggaccc cgtgttaggc tcgcccgcgt gacccgcccc
     3001 cgctccgagc cgcggacgac ctccctcgcg ccacgtgttg ccattttggc gctggcgctg
     3061 acgagcccgc tgactgttct ggcgctcgcc accctcgcgc aggcttgcgg gggcgagccc
     3121 gccaccgacg cggcagcgcc ggccgagccg gccaagcccg gtgcgatcga cacccaggcc
     3181 ggcgcgatcg cccacgccgg cgagcagcgg gccgcccagg cgcgcggccg cgtcgacgcc
     3241 ttgctcgccg agccgacccc cgcgaccctg ctcgccgtgc tcgcccaggg ccacgccgag
     3301 gcgcgcgccc tgctcggccc gcacaacctc cgctacaagg ccagcttcgc cctgaccccg
     3361 gagatcgcga cccgccccgt cgtcgacgag ccgatccagc aagagcagcg cgtcgtcgac
     3421 gagc
//
LOCUS       DASWNJ010001224         6070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_233611, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001224 DASWNJ010000000
VERSION     DASWNJ010001224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6070
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233611"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1507
                     /locus_tag="VGB85_20640"
     CDS             <1..1507
                     /locus_tag="VGB85_20640"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEY0136508.1"
                     /translation="PFVLQSAEAMKTAVRHLEPHLSRAAGPRKGCMVLATVKGDVHDI
                     GKNLVDIILSNNGYEVVNLGIKQPLDNILAAAREHKPDVIGMSGLLVKSTVIMKENLE
                     AMREQGVAMPVICGGAALNRGYVDHNLQNAYSGNEEAKPAAGATPVPEASPEAEDAPR
                     MQAARPRARVFYAKDAFEGLALMDELCGHVAPDKITLTGPKPRKAYRHTTQEEYEAHL
                     KKAIETYVKSDVQPVSRVPTPPFWGARVVGPEELQLGEIFRYINKKALFTGQWQYRRG
                     RLNEAEYEDLIHNTVEPKFRMWCERVIVDKMLEPRVVYGYFPCQSDRNDLLIYDPEDK
                     APPLRIAFPRQLEKGRLCIADFFRSVRSGERDVIALTCVTMSEIATRLSGEMFAADRY
                     DDYLHYYGLGVESAEALAELWHKRVRRELNIAQDDATDIRDLFKQHYQGSRYSFGYPA
                     CPRLEDQVPLVKLLRTDRIGVSLTEEGFQLVPEQSTSAIIVHHPDAKYFNI"
     gene            complement(1550..2623)
                     /locus_tag="VGB85_20645"
     CDS             complement(1550..2623)
                     /locus_tag="VGB85_20645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136509.1"
                     /translation="MRWLLLVAGVCAGCSGSSGETDTTGSSGGLTDGTGSETGGPTTS
                     TTASDPTSSTGVTTEDATSGATGTGTTGGSSDPTTGGSSEATATDTTMGVTDTGDTSD
                     TTNPSGEPIGGCGKVPDVSGSSMQKITAAGMERDYILSMPADYDPSKAYPLVFAWHGR
                     GGNGALAQLYYKIEEAAQGEAIFVYPDGLPLADMANQTGWDLDPNNEDFALFDALFSE
                     VQSRLCIDPERIFSTGHSFGGYMSNQLGCHRGDVVRAIGEVAGGGPFGGCTGQVATWL
                     AHGDLDMVVPFSEGENSRDHWTAANSCGATADPVDPAPCTAFTGCDDGFPVVWCHHSE
                     PDFSGHGWPTWVGPAIWAFFAQF"
     gene            complement(2674..3621)
                     /locus_tag="VGB85_20650"
     CDS             complement(2674..3621)
                     /locus_tag="VGB85_20650"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEY0136510.1"
                     /translation="MLAPLLRAGQTGEIPDPALTAQIAAQAERITALSPAEVYTLLTP
                     ALCGHHPEVAMQWLRDAGLLIHLIPELDATVAFSQEGGRRHKDVWEHTKAVVRQAVPR
                     PAVRWAAVLHDIGKVPTRRFVGPGKVTFHGHAEEGVRMFRRGPRRRIGFPADMVERVE
                     LLILYHLRGGQYDSSWTDAAVRRFAHEMGPVLTDLLDLSRADVTSKRPGKRQRCLCLI
                     SELAKRIRDIAAEDARVPPLPPGLGNLLMTALGLPPGKHIGVLRGQLEGLCEAGEIDA
                     RQPLEYYVEVVRSRGLADGLTVVPPRGIAAPAGVEDPAR"
     gene            3841..4542
                     /locus_tag="VGB85_20655"
     CDS             3841..4542
                     /locus_tag="VGB85_20655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136511.1"
                     /translation="MAGHQSPQLGYNHNIPHRGRLYHVQTEDSGVARAHIFTHVFYDG
                     TIVASNRVDYKPRLESENLDAVVIGLMQESHKSMIRQLRSGAYDEKIVRFIGAHPEGT
                     NKVEAPAAAQPDRKPRPDRRPPPEPRDVEPQGPPLQAGAAGPTIDPGLTHPVFSTPEA
                     QAQVAAAAAGKGPARPSRGPARVGRRMLGGIGGQGVTEGTPIAQRRDIVVGKFASEHQ
                     YQLDDEILALLAEEQ"
     gene            4549..4857
                     /locus_tag="VGB85_20660"
     CDS             4549..4857
                     /locus_tag="VGB85_20660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEY0136512.1"
                     /translation="MGIKNAHTTFKQPQELGADVVIYVTEYCGFCRMAEGLLQRKSIP
                     FEAVDVTNSSDARSWLIEQTGQRTVPQIFIKGQSIGGYSELSALDRAGKLMPMLGGGS
                     "
     gene            5119..5856
                     /locus_tag="VGB85_20665"
     CDS             5119..5856
                     /locus_tag="VGB85_20665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HEY0136513.1"
                     /translation="MTNRLPMQSHRRWTLPTAVALATLCSTAASVAWAGEIKVGASAL
                     EVDDKGNLTAAGKKAAVDTIDEVPGEDTWQVNVWAKLDNAAEGPLYFEFYQSIGGSES
                     LVWRHEEPNFPGGKYFSGEIELDGGRGFNKDRTYEIKAVQVSPKGKDLTLATGKVKLI
                     KSGKKPAKTEGDDEDKVPEDQDAHDSLAGGDEPAGDEPAAGGETPPPVEPKAKKGCTV
                     DSDDSAWNGALLLALLGAGLVARRRRS"
BASE COUNT          983 a         2212 c         2001 g          874 t
ORIGIN      
        1 gccctttgtc ctgcaatccg cggaggcgat gaagaccgcc gtccgccacc tcgagcccca
       61 cctttcgcgc gccgccggcc cgcgcaaggg ctgcatggtg ctcgccaccg tcaagggcga
      121 cgtccacgac atcggcaaga acctcgtcga catcatcctg agcaacaacg gctacgaggt
      181 cgtcaacctc ggcatcaagc agccgctcga caacatcctc gcggccgccc gcgagcacaa
      241 gccggacgtc atcggcatga gcggcctgct ggtcaagtcg accgtgatca tgaaggagaa
      301 cctcgaggcg atgcgcgagc agggcgtcgc gatgccggtg atctgcggcg gcgccgcgct
      361 caaccgcggg tacgtcgacc acaacctgca gaacgcctac agcggcaacg aggaggccaa
      421 gcccgccgcc ggcgcgacgc ctgtccccga ggccagcccc gaggccgagg acgccccgcg
      481 catgcaggcc gcgcgcccgc gtgcgcgcgt cttctacgcc aaggacgcct tcgagggtct
      541 ggcgctgatg gacgagctgt gcggccacgt cgcgcccgac aagatcaccc tgaccggccc
      601 caagccgcgc aaggcctacc gccacaccac gcaggaggag tacgaggcgc acctcaagaa
      661 ggcgatcgag acctacgtca agagcgacgt gcagccggtc tcacgcgtcc cgacgccgcc
      721 gttctggggc gcgcgcgtgg tcggcccgga ggagctccag ctcggcgaga tcttccgcta
      781 catcaacaag aaggcgctgt tcacgggtca gtggcagtac cgccgcggtc ggctcaacga
      841 ggccgagtac gaggacctga tccacaacac cgtcgagccc aagtttcgca tgtggtgcga
      901 gcgcgtcatc gtcgacaaga tgctcgagcc gcgggtcgtc tacggctact tcccgtgcca
      961 gagcgaccgc aacgacctcc tgatctacga ccccgaggac aaggccccgc cgctgcgcat
     1021 cgccttcccg cgccagctcg agaagggtcg cctgtgcatc gccgacttct ttcgcagcgt
     1081 ccgctccggc gagcgcgacg tcatcgccct cacctgcgtc accatgagtg agatcgccac
     1141 gcgcctcagc ggcgagatgt tcgccgccga ccgctacgac gactatcttc attattacgg
     1201 cctcggggtc gagagcgccg aggccctcgc cgagctgtgg cacaagcgag tccgccgcga
     1261 gctcaacatc gcccaggacg acgccaccga catccgcgac ctttttaaac agcactacca
     1321 gggctcgcgc tacagcttcg gctaccccgc ctgccctcgc ctcgaggacc aggtccccct
     1381 cgtcaagctg ctgcgcaccg accgcatcgg cgtcagcctg accgaggagg gcttccagct
     1441 ggtccccgag cagtcgacca gcgcgatcat cgtccaccac ccggacgcga agtacttcaa
     1501 tatttgaagc cctctccgac ctgtcccgcg ggacaggtcg ggggcagcct cagaactggg
     1561 cgaagaacgc ccagatcgcc ggcccgaccc acgtcggcca gccgtgcccg ctgaaatccg
     1621 gctcgctgtg gtgacaccag acgaccggga agccgtcgtc gcacccggtg aacgcggtgc
     1681 acggggccgg gtcgaccggg tcggccgtgg cgccgcagct gttggccgcc gtccagtggt
     1741 cgcggctgtt ctcgccctcg ctaaacggca cgaccatgtc gaggtcgccg tgggccagcc
     1801 aggtcgcgac ctggccggtg cagccgccga acggaccgcc gccggcgacc tccccgatcg
     1861 cgcggacgac atcgccgcga tggcagccga gctggttcga catgtaaccg ccgaagctgt
     1921 gcccggtgct gaagatccgc tcggggtcga tgcacaggcg cgactgcacc tcgctaaaca
     1981 gcgcgtcgaa cagcgcgaag tcctcgttgt tcgggtcgag gtcccagccg gtctggttgg
     2041 ccatgtcggc cagcggcaag ccgtcggggt acacgaagat cgcctcgccc tgcgcggcct
     2101 cctcgatctt gtaatagagc tgcgcgagcg ccccgttgcc gccgcggccg tgccacgcga
     2161 acaccagcgg gtaggccttc gacggatcgt agtcggccgg catcgacagg atgtagtcgc
     2221 gctccatgcc ggccgccgtg atcttctgca tcgacgatcc gctgacgtcc ggcaccttgc
     2281 cgcagccgcc gatcggctcg ccggacgggt tggtcgtgtc cgacgtgtcg cccgtgtccg
     2341 tcacgcccat cgttgtgtcg gtcgccgtcg cctcgctcga cccgccggtc gtcgggtcac
     2401 tcgagccacc ggtcgtcccg gtccccgtcg cacccgaggt cgcgtcctcg gtcgtcacgc
     2461 ccgtcgagct ggtcgggtcg ctcgcggtcg tcgacgtcgt cggcccgccg gtctccgaac
     2521 ctgtcccatc ggtcagccca ccgctcgagc ccgtcgtgtc ggtctcgcca ctggaccctg
     2581 aacaacccgc gcacacgccc gcaacaagga gaagccatcg catgccgcga cggtaccgca
     2641 gcgacggccg caaacccagc cgcggcgccc ccttcagcgc gcgggatcct cgacgccggc
     2701 cggggccgcg atcccccgcg ggggcacgac cgtcaggccg tccgcgagcc cgcgcgagcg
     2761 cacgacctcg acataatatt cgagcggctg gcgagcatcg atctcgccgg cctcgcacag
     2821 cccctcgagc tggccgcgca ggaccccgat gtgcttgccg ggcggcaggc cgagggccgt
     2881 catcaacagg ttgccgagcc cggggggcag cggcggcacg cgggcgtcct cggccgcgat
     2941 gtcccggatc cgctttgcca gctcggagat caggcacagg cagcgctggc gcttgccggg
     3001 ccgcttgctg gtgacgtcgg cgcgcgacag gtcgagcagg tcggtcagca ccgggcccat
     3061 ctcgtgggcg aagcggcgca ccgcggcgtc cgtccagctg ctgtcgtact gcccgccgcg
     3121 caggtgatac aggatcagca gctcgacgcg ctcgaccatg tcggccggaa acccgatccg
     3181 ccgccgcggg ccgcgacgga acatccgcac gccctcctcg gcgtggccat gaaacgtcac
     3241 cttgccgggg ccgacgaagc gccgcgtcgg caccttgccg atgtcgtgca gcaccgccgc
     3301 ccagcgcacc gccggccgcg gcaccgcctg gcgcacgacc gccttggtgt gctcccagac
     3361 gtccttgtgg cgccggccgc cctcttgcga gaacgcgacg gtcgcgtcga gctcggggat
     3421 gaggtgtatg agcaggccgg cgtcgcgcag ccactgcatc gcgacctcgg ggtggtgtcc
     3481 gcacagcgcc ggggtcagca gcgtgtagac ctcggcgggc gacagggcgg tgatccgctc
     3541 ggcctgggcg gcgatctgcg cggtgagcgc gggatcgggg atctcgccgg tttggcccgc
     3601 ccggagcagc ggagcgagca tttcgccggg ttcgaggagg gtggaggcgg gagcggcaga
     3661 cacggtggct tcaccggccg cgatcggtcg ggtcgcggag cataccaggg gaggcgcggc
     3721 gggtctgtgg ccgagttggg cggggcaccc gaacggggaa aagccggcgg gattcgcacg
     3781 ctggacgcac cggagggctc tcggcgaggg tgctcccgtg ttagccttgg agccaccccc
     3841 atggccggcc atcagtctcc gcagctagga tacaaccaca acatcccgca ccgggggcgg
     3901 ctctatcacg tgcagaccga ggattcgggg gtcgcacgcg cgcacatctt cacgcacgtg
     3961 ttctacgacg gcacgatcgt cgcttccaac cgggtcgact acaagcccag gctcgagagc
     4021 gagaacctcg acgcggtcgt catcggcttg atgcaggagt cgcacaagtc gatgatccgc
     4081 cagcttcgca gcggcgcata cgacgagaag atcgttcgct tcatcggcgc gcaccccgag
     4141 ggtaccaaca aggtcgaggc gccggccgcc gcgcagcccg accgcaagcc ccgccccgac
     4201 cgccgcccgc cgcccgagcc gcgggacgtc gagccgcagg gccccccgct ccaggccggc
     4261 gcggccggcc cgacgatcga tccggggctc acgcacccgg tgttctcgac ccccgaggcc
     4321 caggcccagg tcgccgctgc ggccgccggc aaggggcctg cgcgaccgag ccgcgggcct
     4381 gcacgcgtcg gtcggcgtat gctgggtggc atcggtggcc agggggtcac cgagggcaca
     4441 ccgatcgccc agcgccgtga catcgtcgtc ggcaaattcg ccagcgagca tcagtaccag
     4501 ctcgacgacg agatcctcgc gctgctggcc gaagaacagt gaggggtcat gggcatcaag
     4561 aacgcgcaca ccaccttcaa gcagccgcaa gagctcggcg cggacgtcgt catttacgtc
     4621 accgagtatt gtggtttttg tcggatggcc gagggtttgc tgcagcgcaa gagcatcccc
     4681 ttcgaggcgg tcgacgtcac gaactccagc gacgcccgca gctggctcat cgagcagacc
     4741 ggccagcgca ccgtcccgca gatctttatc aaggggcaga gcatcggtgg gtacagcgag
     4801 ctctccgcgc tcgaccgcgc tggcaagctg atgccgatgc tcggcggtgg gtcttgatct
     4861 gcccgtgaat ctgctctgat ctttgcgcgc acctcatccg cgccggacag gtgtacaagc
     4921 aacgctggcg cgcgcgatcg attggttcca gttcgctcgc cccgccagtg cggcctcata
     4981 cgtcgatcat tgcaacgttc gaccgggatg gacgtacact gctgggcagt tcgtccccac
     5041 gacgaacggc gacgggcgat cacgagtctt cgtggtactc tcccgccgct tcgatcgacg
     5101 agactagaga tgaccgccat gaccaatcga ctgcccatgc aatctcaccg acgctggacc
     5161 ctccccacgg ccgtggcgct ggcgaccctc tgtagcaccg cggcctccgt cgcctgggcg
     5221 ggcgagatca aggtcggcgc ctcggcgctc gaggtcgacg acaagggcaa cctcaccgcc
     5281 gccggcaaga aggccgccgt cgacacgatt gatgaggtcc cgggcgagga cacctggcag
     5341 gtgaacgtgt gggccaagct cgacaacgcc gccgagggcc cgctctactt cgagttctat
     5401 cagagcatcg gtggcagcga gtcgctggta tggcgccacg aggagcccaa cttccccggc
     5461 ggcaagtact tcagcggcga gatcgagctc gacggcggcc gcggctttaa taaagaccgc
     5521 acctacgaga tcaaagccgt ccaggtcagc cccaagggca aggacctcac cctcgccacc
     5581 ggcaaggtga agctgatcaa gagcggcaag aagcccgcga agaccgaggg cgatgacgag
     5641 gacaaggtgc ccgaggacca ggacgctcac gactccctcg ctggtggcga cgagcccgcc
     5701 ggcgacgagc ccgccgctgg cggcgagacc ccgccccccg tcgagcccaa ggccaagaag
     5761 ggctgcaccg tcgacagcga cgactccgcc tggaacggcg ccctgctgct ggccctcctc
     5821 ggcgccggcc tggtcgcccg ccgccgccgc agctgagccg ccccgacgta ccgcccccga
     5881 gccccgtgtc ctccggacgc ggggctcttt gcgttttccc gactggtgtc tccggacgca
     5941 ggcctctctt gggtgcctcg cctcgggatc ttcccgacgc ggggctcttt gcgttcccgc
     6001 tggctcgtct cccagctcat gtcctccgga cgcgggcctc cgtgcgtgtc ccggctcgtc
     6061 ttccggacgc
//
LOCUS       DASWNJ010001225         2773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_58335, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001225 DASWNJ010000000
VERSION     DASWNJ010001225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58335"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VGB85_20670"
     CDS             complement(<1..525)
                     /locus_tag="VGB85_20670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136514.1"
                     /translation="MPDSSSNRPWRSRTFDWPVDRLRIDVEVVEPRLLTALEWVILRV
                     ADEFPDATPTLSEVADELGLDRPEFLHDTLREVVRLRALAPRDPDVPIRDLGDVAFTD
                     SGRALYRRGQIEADPATHAVLVDIDALTDEDLKAGERKPMASSPVLADPPPRQSIGLD
                     RLRSVMRRFHADIVR"
     gene            complement(503..>2773)
                     /locus_tag="VGB85_20675"
     CDS             complement(503..>2773)
                     /locus_tag="VGB85_20675"
                     /inference="COORDINATES: protein motif:HMM:NF024486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HEY0136515.1"
                     /translation="RAERDAELRANNLEARLVAAMQAHEQAVLAVDAARVHEAAARRL
                     AAWAAGAAFPTSLPADVRFADRSIEQLAELATVAATREPLTRLDQVLAGVRLDASVHE
                     TSPEVTALRREQRELRESDHDDDLAKLKEINRRLKKLEESGWLATGRQLRQAAGDAFL
                     SGVPTCIERLIDALKPEPGLAGDLEQAQQVVRTRLQTAVTAQAIVAELAGPLAAGALA
                     SQESHSLTRALQDRRAHELVALRDALAAAHESREAAAQQRHAAVAGWHAAHRILDRES
                     PAPAIIASALQEAADRVVAANTADDAQLRRANRWRKIQSEWLARLQTITEADRDHLKD
                     LYIRHANVVGMTCNEAGKREHWQSPEWRPFDLVIIDEVSKATPTELLLPMLLGARVVL
                     VGDHRQLAPMFRESRDSFAEAIESGELAAADFDRFRRMVTASLFEELYAQAPETIKAT
                     LWTQYRMHPQIMHAVNQFYEGRLTAGPDEQSLDRTRQHHLQAADRDGGRFLEPGQHLL
                     WIDSSEHEGVPRIEEQRGSSKFNRLEVDLVLATLTTLARALHGRGFVGVRERTLDETD
                     ADRTMAALAAHLLPGAPAETLDELFAERRVRLDGRSQGPDALGQPGALLHIDARKEVG
                     VLTFYFAQLQDIRRAIDSALAKSATIFDALDLRSNTVDRFQGMEKAIVIASLVRATRG
                     QLGDFVREYQRINVGLSRAQQLLVVIGAVDTWKRARVPLPPLTGGEAEMRPVYADIFE
                     IARRHGGRRIARQLLE"
BASE COUNT          412 a          887 c         1024 g          450 t
ORIGIN      
        1 gcggacgatg tcggcatgga accggcgcat caccgagcgc aggcggtcga ggccgatgga
       61 ttgacgaggc ggtgggtcgg cgagcacggg gctgctcgcc atcggcttcc gctcgccggc
      121 cttcaggtct tcgtcggtga gggcatcgat atcgaccagg acggcgtggg tggccggatc
      181 ggcctcgatc tgaccgcggc ggtagagcgc acggccgctg tcggtgaagg cgacatcgcc
      241 gaggtcccgg atcggcacgt ccgggtcccg cggcgcaagg gcccgcaggc gcacgacctc
      301 gcggagggtg tcgtgcaaga actcggggcg atcgagaccg agctcgtcgg cgacctcgga
      361 gagcgtcggg gtagcgtccg ggaactcgtc ggcgacccgc aggatgaccc actcgagcgc
      421 cgtaagcagg cgcggctcga cgacctcgac gtcgatccgc aggcgatcga cgggccagtc
      481 gaacgtccgg ctgcgccatg gcctattcga ggagctgtcg ggcaatacgt ctccctccgt
      541 ggcgcctggc gatctcgaaa atatcggcat agacggggcg catctcggcc tcaccaccgg
      601 tgagcggcgg cagcggcacg cgagcgcgct tccacgtgtc gaccgcgccg atgacgacga
      661 gcagctgctg ggcgcgcgag agaccgacgt tgatccgctg atactcgcgg acgaagtctc
      721 cgagctgtcc gcgcgtcgcg cggaccaggc tggcgatcac gatcgccttc tccatgccct
      781 ggaaacggtc gaccgtgttg gagcgcaggt ccagcgcgtc gaagatcgtg gcgctcttgg
      841 cgagcgcgct gtcgatggcg cggcggatgt cttgcagctg agcgaagtag aaggtgagga
      901 ccccgacctc cttgcgcgca tcgatatgca gcagcgcacc gggctgtccc agggcgtcgg
      961 ggccttggga tcgcccgtcg agtcgcacgc ggcgctcggc gaacagctca tcgagcgtct
     1021 ctgcaggcgc ccccgggagg agatgggccg cgagggccgc cattgtgcga tcggcgtcgg
     1081 tctcgtcaag ggtgcgctcg cggacgccga cgaagccgcg gccatggagc gcgcgcgcca
     1141 gggtcgtcag ggtggcgagc acgaggtcga cctcgagccg attgaacttc gagctgccac
     1201 gctgctcctc gatccggggc accccttcat gctccgatga atcgatccat aacagatgct
     1261 ggcctggctc gaggaaacgg ccgccgtcgc ggtcggccgc ctgcagatgg tgctggcgag
     1321 tccggtcgag cgattgttca tcggggccag cggtgagtcg gccctcgtag aattggttga
     1381 ccgcgtgcat tatctgcggg tgcatgcggt actgtgtcca cagcgtcgcc ttgatcgttt
     1441 cgggagcctg agcatagagt tcctcgaaca ggctcgcggt gaccatcctg cgaaacctgt
     1501 cgaagtcggc ggccgcgagt tcgccgctct cgatcgcttc agcgaagctg tcccggctct
     1561 cgcggaacat cggcgccagc tgacggtgat ctccgacgag gacgacgcgc gcgccgagta
     1621 gcatcggcag gagaagctcg gtcggggtgg ccttgctgac ttcgtcgatg atgaccaggt
     1681 cgaagggccg ccactccggc gactgccagt gctcgcgctt tccggcctcg ttgcaggtca
     1741 tgccgaccac gttggcgtgg cgaatataga ggtctttgag gtgatcacgg tcagcctcgg
     1801 tgatcgtctg cagacgcgcg agccactcgc tctggatctt ccgccaccgg ttggcgcgcc
     1861 gcagctgggc atcgtcggcg gtgttggccg cgacgacgcg gtctgcggcc tcctgaagcg
     1921 cgctggcgat gatcgcgggt gcaggactct cgcggtcgag gatccgatgg gctgcatgcc
     1981 agccagctac ggccgcgtga cgctgctgcg cggcggcctc gcgcgactca tgagctgcgg
     2041 cgagcgcgtc acggagcgcg acgagctcat gcgcgcgacg gtcctggagc gcgcgcgtca
     2101 gggagtgcga ctcctgcgac gccagggcgc cagcggcgag aggcccggcg agctcggcga
     2161 cgatcgcctg ggcggtgacg gccgtctgaa gccgagtacg gacgacctgc tgcgcctgtt
     2221 ccaggtcccc cgccaggccg ggctcgggct tcagggcgtc gatcaggcgc tcgatgcagg
     2281 tgggtacacc ggacaggaac gcatcaccgg cggcctggcg gagctgccga ccggtcgcta
     2341 gccagccgct ctcctcgagc ttcttgagac ggcggttgat ctccttgagt ttggcgaggt
     2401 cgtcatcgtg atctgactcg cgaagctctc gctgctcccg acgcagcgcg gtgacctcgg
     2461 gcgatgtctc gtggacggag gcgtcgaggc gcacgcctgc gaggacctgg tcaaggcggg
     2521 tcaatggctc gcgggtggcg gcgacggtgg cgagctcggc cagctgctcg atcgagcggt
     2581 cggcaaaccg aacgtcggcc ggcaatgagg tcgggaacgc tgcgccggcg gcccaggcgg
     2641 cgagcctgcg agcggcggcc tcatggacgc gggcggcgtc gacggctagc accgcctgct
     2701 cgtgtgcctg catggcagcg acgagcctcg cttcgaggtt atttgcgcgg agttcggcgt
     2761 cgcgctcggc tcg
//
LOCUS       DASWNJ010001226         2590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_22876, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001226 DASWNJ010000000
VERSION     DASWNJ010001226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22876"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..1902
                     /locus_tag="VGB85_20680"
     CDS             73..1902
                     /locus_tag="VGB85_20680"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY0136516.1"
                     /translation="MVARIEDYALIGDCHSAALVSREGSIDWLCLPRFDAPACFAALL
                     GTREHGRWCITPRGRFETTRWYVPGTMVLVTEFRGPAGVCRLIDCMVVEDTHPTLVRT
                     VEGVHGSVDLELELIIRFDYGSIVPWIRRRRDGSLLAIAGPDNLLLHTPVPLRGENLR
                     TCATFTVHAGERVPFTLVWHPSHLDHPPPPTDPAAMVAHTIAWWQAWSSRCTYAGPHR
                     DLVQRSLLTLKALTYAPTGGIVAAPTTSLPELIGGVRNWDYRYCWLRDATFTLYALLG
                     AGYREEASRWREWLIRAVAGTPSQINIMYGLSGERRLTELELPWLPGYAGSRPVRIGN
                     AACAQLQLDVFGEVMDTLHLARRSGLEYSESSWRVQRAMLEYLESAWDQPDEGIWEVR
                     GPRRHFTHSKVMAWVAFDRGIKAVQGFGRPGPVARWRAVRQQIFEQVCRYGYDPELDS
                     FVQSYGSREVDASLLMMSLVGFLPANDPRIVGTVAQIERTLMHGGLLQRYRPAHTADG
                     LPGGEGAFLACSFWLADNYILQGRRADAERLFSKLAGLCNDVGLLSEEYDPVQRRFLG
                     NFPQAFSHIALVNTAINLSRRGSAADDRSHVPGGPRTPGQASG"
     gene            complement(1899..2570)
                     /gene="udk"
                     /locus_tag="VGB85_20685"
     CDS             complement(1899..2570)
                     /gene="udk"
                     /locus_tag="VGB85_20685"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEY0136517.1"
                     /translation="MSATIVGIAGGSGSGKTTIARKIAESLPSDGVGIIEHDAYYRDR
                     QDLSFEERCQLNFDHPESLETELLVEHLIALKAGRRVAVPVYDFKTHRRTDDPRWLDA
                     SPVIIVEGILVLVDARLRAELDIKIYVDTDPDIRIFRRIRRDIEQRGRTFESVREQYY
                     RHVRPMHLQFVEPAKRRADLIIPEGGENRVALDLVTSKLRSIIDRSREGVAAAGADER
                     SRAGR"
BASE COUNT          378 a          938 c          866 g          408 t
ORIGIN      
        1 gcggaggctc tgctatgagt cggacgtgca cgcaccggct cgcaccgcaa ccccaggagc
       61 ccacgggcgc tgatggtcgc gcgcatcgag gactacgcgc tgatcggcga ctgccacagc
      121 gccgcgctcg tcagccgcga ggggtcgatc gactggctgt gcctgccgcg cttcgacgcc
      181 ccggcctgct tcgccgcgct gctcgggacc cgtgaacacg gccgttggtg catcacgccg
      241 cgcggccgct tcgagacgac ccgctggtac gtgcccggca cgatggtgct cgtgaccgag
      301 ttccgcggcc ccgccggcgt gtgccggctg atcgactgca tggtcgtcga ggacacgcac
      361 ccgacgctgg tgcgcaccgt cgagggtgtg cacggcagcg tcgacctcga gctcgagctg
      421 atcattcgct tcgactacgg gtcgatcgtg ccgtggatcc gccgccgccg cgacggctcg
      481 ctgctcgcga tcgccggccc cgacaatctg ctcctgcaca cgcccgtccc gctgcgcggc
      541 gagaacctgc gcacctgcgc cacgttcacc gtgcacgccg gcgagcgcgt gccgttcacg
      601 ctggtgtggc acccctcgca cctcgaccac ccgcccccgc cgaccgaccc ggcggcgatg
      661 gtggcccata cgatcgcgtg gtggcaggca tggtcgtcgc gttgcactta cgccggcccg
      721 caccgcgacc tcgtgcagcg ctcgctgctg acgctcaagg ccctgaccta cgcgccgacc
      781 gggggcatcg tcgcagcgcc gacgacctcg ctgccggagc tgatcggcgg cgtgcgcaac
      841 tgggactacc gctattgctg gctgcgcgac gcgaccttca ccctgtacgc cctgctcggc
      901 gccggatacc gcgaggaggc gagccgctgg cgcgaatggc tgatccgcgc cgtcgccggc
      961 accccgagcc agatcaacat catgtacggc ctgtccggcg agcgccgcct caccgagctc
     1021 gagctgccgt ggctgcccgg ctacgccggc tctcgcccgg tccggatcgg caacgccgcg
     1081 tgcgcgcagc tgcaactcga cgtattcggc gaggtcatgg ataccctgca cctcgcccgc
     1141 cgctccggcc tcgagtacag cgagagctcg tggcgagtcc agcgcgcgat gctcgagtac
     1201 ctcgaatccg cgtgggacca gcccgacgag ggcatctggg aggtccgcgg cccgcggcgc
     1261 cacttcaccc actccaaggt gatggcctgg gtcgccttcg accgcggcat caaggccgtg
     1321 caaggcttcg gccgcccggg acccgtcgcc cgctggaggg cggtgcgcca gcagatcttc
     1381 gagcaagtgt gtagatatgg ttacgacccg gagctcgaca gcttcgtgca gtcctacggc
     1441 agccgcgagg tcgacgcctc gctgttgatg atgagcctcg tcggcttcct gcccgccaac
     1501 gacccgcgca tcgtcggcac cgtcgcgcag atcgagcgca ccctgatgca cggcggcctg
     1561 ctccagcgct accgcccggc gcacaccgcc gacggcctgc ccggcggcga gggggcgttc
     1621 ctcgcctgct cgttctggct ggccgacaac tacatcctgc aaggccgccg cgccgacgcc
     1681 gagcgcctgt tttcaaaact cgccggcctg tgcaacgacg tcggcctgct ctccgaggaa
     1741 tacgacccgg tgcagcgtcg attcctcggc aactttcccc aggccttctc acatatcgcg
     1801 ctcgtcaaca ctgcgatcaa tctctcgcgg cggggcagcg ccgcggacga ccgcagccat
     1861 gtacccgggg gaccccgaac acctgggcag gcgagcggct agcgcccggc ccgggaccgc
     1921 tcgtcggccc cggcagcagc aaccccttcg cgcgagcgat cgatgatgct gcgcagcttg
     1981 gaggtcacca gatcgagcgc gacgcggttc tcgccgcctt cggggataat aaggtcggcg
     2041 cggcgcttgg cgggctcgac gaattgcagg tgcatcggcc ggacgtggcg gtagtactgc
     2101 tcgcgcaccg actcgaaggt gcggccgcgc tgctcgatgt cacggcgtat acggcgaaag
     2161 atgcggatgt ccgggtcggt gtcgacgtag atcttgatgt cgagctcggc gcgcaggcgg
     2221 gcgtcgacga gcacgaggat gccctcgacg atgatcaccg gcgaggcgtc gagccagcgc
     2281 gggtcgtcgg tgcggcggtg ggtcttgaag tcgtagaccg gcaccgcgac gcgccgtccc
     2341 gccttgaggg cgatcaggtg ctcgacgagc agctcggtct ccagcgactc cggatgatcg
     2401 aagttaagct gacagcgctc ctcaaagctg aggtcctgac gatcgcggta gtacgcgtcg
     2461 tgctcgatga tcccgactcc gtcggagggc agactctcgg cgatcttgcg agcgatcgtc
     2521 gttttgcccg agccgctgcc gccggcgatc cccacgatgg tcgcgctcat ggtgcccaga
     2581 taccacagtt
//
LOCUS       DASWNJ010001227         2449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_514739, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001227 DASWNJ010000000
VERSION     DASWNJ010001227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514739"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VGB85_20690"
     CDS             complement(<1..657)
                     /locus_tag="VGB85_20690"
                     /inference="COORDINATES: protein motif:HMM:NF016341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(His) guanylyltransferase Thg1 family
                     protein"
                     /protein_id="HEY0136518.1"
                     /translation="MTSETSPESTGDDPLGDRMKAYEMVEAGRRAEPGLPILVRLDGK
                     AFHTFTKGLERPFDARLQGLMDATTRHLVKESGAIVGYTQSDEISLVLHTATARSEHY
                     LGGRFQKLCSILAAMATSVFGRRLAEALPEKADKSPIFDARAWTVPTLEEAANTLLWR
                     EQDATKNSIAMAAQARLSHTQLQGKSKREQQALLLATHGVDWNDYPARSKHGAWFRRV
                     E"
     gene            complement(722..2386)
                     /locus_tag="VGB85_20695"
     CDS             complement(722..2386)
                     /locus_tag="VGB85_20695"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEY0136519.1"
                     /translation="MLSSQASLVDPRGPAYLRAKPFPPLLAALLALCACPAGSGETTE
                     SSADATTSTGTAAPTSTTGTTGDPNTGTTGAPTTGPTSTTAATTTTTDETTTGAEPTT
                     GGSETTGAGSSTGEPAIPPECDGIAPSPDPAVNHAAILACLNNFGVARLAPGEFPVAA
                     EIDMPAGSRLLGDATWPRIKMVAKAQSLVRVHDDCEVAFLRLDGNDQMTVAHNAIVRL
                     LGNNGFVHDNHVQNGDGVASLTDHVTGVRFWDPTVTGNRVFRNQIHHVHYGVIFDLYK
                     NGADNLLEDNKIYEVRCDGVTFRGYGRAHGNEIHHVGFQCLNPPESPIPGGGFYTLNN
                     FEGFEIVGNHVHETCGQPLDLDRAANLVIKDNLIEKPGFSWDGHKCGAGIAGHLLDIR
                     DSLIEGNTFRNTGFTTIKSDPNHVMSADGSGVPSDMPSGANQAVAFALTHREGKPETS
                     THNTIKNNKFIANCQAPCVGLGYFIGRGTGFAANKTWSADTTNYFTGNSAFGSNIGSK
                     RCGGGWFAANSDCKEGMLEPECNADDPQHEGPGHDWTRSDNCPHYQ"
BASE COUNT          310 a          766 c          922 g          451 t
ORIGIN      
        1 ctcgacgcgg cgaaaccatg cgccgtgctt gctgcgcgcc gggtagtcgt tccagtcgac
       61 gccgtgggta gcgagcagca gcgcctgctg ctcgcgcttg ctcttgccct gcagctgcgt
      121 gtgcgacagc cgggcctgcg cggccatggc gatgctgttc ttggtcgcgt cctgctcgcg
      181 ccacagcagc gtgttggcgg cctcctcgag ggtcggcacg gtccacgcgc gggcgtcgaa
      241 gatcggcgac ttgtcggcct tctcgggcag cgcctcggcg agccgccggc cgaacacgga
      301 ggtcgccatc gccgcgagga tcgagcacag cttttgaaac cggccgccga ggtaatgctc
      361 ggacctcgcg gtcgcggtgt gcagcaccag cgagatctcg tcggactgcg tgtagccgac
      421 gatcgcgccc gactccttga cgaggtggcg cgtggtcgcg tccatcaacc cttgcaggcg
      481 cgcgtcgaag ggccgctcga ggccttttgt gaaggtgtgg aaggccttgc cgtcgaggcg
      541 cacgaggatc ggcaggccgg gctcggcgcg acggccggcc tcgaccatct cgtaggcctt
      601 catgcggtcg ccgagcggat cgtcgccggt cgactcgggg ctcgtctcgc tggtcatggg
      661 ccgcgcatga caacagatcg ccggaccggc gacaaccggc gtacggcatg cgcggcggcg
      721 ctcactggta gtgcgggcag ttgtcgctgc gcgtccagtc gtggccgggg ccctcgtgct
      781 gcgggtcgtc ggcgttgcac tcgggctcca gcatgccctc cttgcagtcg ctgttggccg
      841 cgaaccatcc gccgccgcag cgcttcgagc cgatgttcga cccgaacgcg ctgttgccgg
      901 tgaagtagtt ggtggtgtcg gcggaccagg tcttgttggc cgcgaagccg gtgccgcgcc
      961 cgatgaagta gccgaggccg acgcacggag cctggcagtt ggcgatgaac ttgttgttct
     1021 tgatcgtgtt gtgcgtcgag gtctcgggct tgccctcgcg gtgggtcagc gcgaaggcga
     1081 cggcctggtt ggccccgctc ggcatgtcgc tcgggacgcc gctgccgtcg gccgacatca
     1141 cgtggttggg gtcgctcttg atcgtggtga agccggtgtt gcgaaaggtg ttgccctcga
     1201 tcagcgagtc gcgaatgtcg agcaagtggc ccgcgatccc ggccccgcac ttgtggccgt
     1261 cccacgagaa accgggcttt tcgatcaggt tgtccttgat cacgaggttg gccgcgcggt
     1321 cgaggtcgag cggctgcccg caggtctcgt gcacgtggtt gccgacgatc tcgaagccct
     1381 cgaaattgtt gagcgtgtaa aaaccgccgc cggggatcgg gctctcgggc ggattgaggc
     1441 actgaaatcc gacgtggtgg atctcgttgc cgtgcgcgcg cccgtagccg cggaaggtga
     1501 cgccgtcgca gcggacctcg tagatcttgt tgtcctcgag caggttgtcg gcgccgtttt
     1561 tatagaggtc gaagatcacg ccgtagtgca cgtggtggat ctggttgcga aacacccggt
     1621 tgccggtgac ggtcggatcc cagaagcgca cgccggtgac gtggtcggtc aggctggcga
     1681 cgccgtcgcc gttttgcacg tgattgtcgt ggacaaagcc gttgttgccg agcaggcgca
     1741 cgatcgcgtt gtgggcgacg gtcatctgat cgttgccgtc gaggcgcagg aacgcgacct
     1801 cgcagtcgtc gtgcacgcgc accagcgact gcgccttggc gaccatcttg atccgcggcc
     1861 aggtcgcgtc gccgagcagg cgcgagccgg ccggcatgtc gatctcggcg gcgaccggga
     1921 actcgccggg ggccaggcgg gcgacgccga aattatttaa acaggcgagg atcgccgcgt
     1981 ggttgacggc cgggtcgggg ctcggcgcga tgccgtcgca ctcgggcggg atcgcaggct
     2041 cgcccgtgct gctcccggcg cccgtggttt cggacccgcc ggtggtcggc tcggcgccgg
     2101 tcgtggtctc gtcggtcgtg gtcgtggtcg cggcggtggt cgaagtcggg ccggtcgtcg
     2161 gcgcgccggt cgtgccggtg ttcgggtcgc cggtcgtgcc ggtcgtgctc gtcggtgcgg
     2221 cggtgccggt gctcgtcgtg gcgtcggcgc tgctctcggt ggtctcgccc gagcctgcag
     2281 ggcatgcgca aagcgccagg agtgcggcca ggagcggtgg gaacggcttt gcgcgcaagt
     2341 acgcggggcc gcgcgggtcg acgagggacg cctgagagga cagcatgacc cgcgagtatg
     2401 cgcgccggga tctgcgcttg cgaggctctg cgctcgcgct gtgcggcgc
//
LOCUS       DASWNJ010001228         3598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_692680, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001228 DASWNJ010000000
VERSION     DASWNJ010001228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692680"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1574)
                     /locus_tag="VGB85_20700"
     CDS             complement(213..1574)
                     /locus_tag="VGB85_20700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0136520.1"
                     /translation="MRTSSTPSSVLARPRGVVLAGLLALGALLPSCNREVSAAKKAPG
                     GPVEVGVMTVTASPVTLTTELPGRTSAVRVAEVRARVNGIVQKRLFTEGSDVKEGQPL
                     FEIDPAPYRATLAGAKAALARAEANRTAARQQASRLEALVASNTASREELERVTATAA
                     TAEADVEAARATLTQAKLNLEYTRVTAPIAGRIGRSTVTEGAYVQQSAATLMATIQQL
                     DPVYVDLTQSTAEVLRLRRAMEAAVLQAPGAETKVTLILEDGRAYAEPGTLQFSDVTV
                     DTGTGSISLRAVFPNPRQELLPGMFVRARLDEGINPSALLVPQRAVTRDPKGQAIALV
                     VTAENKVERRTLVTDRAVGNAWLVSQGLAPGDQVILEGLQKVRPGADVIAVPAAPDAP
                     PAQPGTPQPAGGAPAGQQAAGQQPGAAQPAAGEPAATTPAAGTTTPAAPASPGSAASP
                     ANR"
     gene            1659..2330
                     /locus_tag="VGB85_20705"
     CDS             1659..2330
                     /locus_tag="VGB85_20705"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0136521.1"
                     /translation="MASTEKLPDPAACSPKRRQILAGARQVFAEMGFERASVDLIAAR
                     TGVSKATLYNHFHDKKALFVACFSEEADALREQVREALLREPEGELATALQAVGEQFL
                     AMILTPAIVSLWRHTSAEAARFPEIGQMLFDRGPGLMIDGIAGYLQRWIARGALRITD
                     VRAAAVQFFMLCHGDLMLRSQLGILPYPADDQIREVVRGAVAVFVRAYQPPHQAESAE
                     LQASP"
     gene            complement(2315..2797)
                     /locus_tag="VGB85_20710"
     CDS             complement(2315..2797)
                     /locus_tag="VGB85_20710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136522.1"
                     /translation="MSDDQDDTANEASDGWTWASEGWTARVQKNEHDEGWAVTMTRDG
                     DAEPILTTHWTMGRDKKNPKPLNVSDFRTLLKGARDVLTRHEAQNRSARHRTVTVTDD
                     AGAAVRVDLDIAADEDDPHAMLTAWDGPEKLGERRVAPNFKLTAAAAGKWVSSGYGDA
                     "
     gene            complement(2915..>3598)
                     /locus_tag="VGB85_20715"
     CDS             complement(2915..>3598)
                     /locus_tag="VGB85_20715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136523.1"
                     /translation="LSRYIQKIRIPRAPSNLDPALVAAGATLFKDVAQGGNCQGCHGG
                     DKWTISRVFYTPSGPTNEALKSKVWDEAAVLATGFPAALLPATTPMNQRMRFGVPAND
                     QLQCIIRNVGTFGVAPPAVGVAELRADMMTAAQGGTVDAKGYNPPSLLGLAVGAPYFH
                     AGNARTLEELLSPTFDAHRKALTKNNSFLLDPTDVQKLVAYLLSIDGTTQAIPIPALG
                     AQGGDFCKQ"
BASE COUNT          472 a         1197 c         1370 g          559 t
ORIGIN      
        1 gacccgcagc ccgtcggccc ccaccccccg gcccacacag cctcgccccg cgctctccga
       61 tcgcgccccg cgatgtccga gccccgcgcg ctccgaacgc gccccgcgat gtccgagccc
      121 cgcgctctcc gaacgcgccc cgcgatatac gagcgccgga gcgcgcggat cactgcatat
      181 cgggtaatac ataggagctc gcatcgaggg attcagcgat ttgcgggcga agcagcggag
      241 ccgggcgagg cgggcgcagc aggcgtcgtg gttccggcgg cgggtgtggt cgcagcaggc
      301 tcaccggcgg caggctgcgc agcgccgggc tgctgaccgg cagcctgctg gccggcaggc
      361 gcgccgccgg caggctgcgg cgtgccgggc tgggcgggcg gcgcgtcggg ggccgcgggc
      421 acggcgatga cgtcggcgcc cggtcgaact ttttgcaggc cctcgaggat cacctggtcg
      481 ccgggcgcga ggccttgact gacgagccag gcgttgccga cggcgcggtc ggtgacgagg
      541 gtgcggcgtt cgaccttgtt ctcggcggtg acgacgagcg cgatcgcctg gcctttgggg
      601 tcgcgggtga ccgcgcgctg gggcacgagc agggcgctgg ggttgatgcc ctcgtcgagg
      661 cgggcgcgca cgaacatgcc gggcagcagc tcctggcgcg ggttggggaa cacggcgcgc
      721 agggagatcg atccggtgcc ggtgtcgacg gtgacgtcgg agaattgcag ggtgccgggc
      781 tcggcgtagg cgcggccgtc ctcgaggatc agcgtcactt tggtctcggc gccgggggcc
      841 tgcaggaccg cggcctccat cgcgcgacgc aggcgcagga cctcggcggt cgactgcgtg
      901 aggtcgacgt agacggggtc gagctgctgg atcgtcgcca tcagcgtggc ggcgctctgc
      961 tgcacgtagg cgccctcggt gacggtgctg cggccgatcc ggccggcgat cggggcggtc
     1021 accctggtgt actcgaggtt gagcttggcc tgcgtcagcg tcgcgcgggc ggcctcgacg
     1081 tcggcctcgg cggtcgcggc ggtcgcggtg acgcgctcga gctcctcgcg gctggcggtg
     1141 ttgctggcga cgagcgcctc gaggcggctc gcctgctggc gcgcggcggt gcggttggcc
     1201 tcggcccggg ccagcgccgc cttcgctccg gcgagcgtcg cccggtacgg ggccgggtcg
     1261 atctcgaaga ggggctggcc ctccttcacg tcactgccct cggtgaacag acgcttttga
     1321 acgatgccgt tgacgcgcgc gcgaacctcg gcgacgcgaa ccgcggaggt ccgacccggt
     1381 aactcggtcg tcaacgtgac ggggctcgcc gtcaccgtca tcacgccgac ctcgaccgga
     1441 ccaccgggcg ccttcttggc cgcagagacc tcgcggttac acgacgggag cagggcaccg
     1501 agcgcgagca ggccggcgag cacgacgccg cgcggacgag cgaggacgga ggacggggtc
     1561 gaggacgtgc gcatgagaag gaaaccggca ggtttcaggc cagaactatg aaccaggcgc
     1621 cttgactgtc aagactgtac cgtacagtat aggtcgcagt ggcctccacc gaaaagctgc
     1681 cggacccggc ggcgtgctcg ccgaagcgcc ggcagatcct cgccggggcg cgccaggtgt
     1741 tcgccgagat gggcttcgag cgggccagcg tcgacctgat cgcggcgcgc accggggtgt
     1801 cgaaggcgac gctctataat catttccacg acaagaaggc gctgttcgtc gcctgcttct
     1861 cggaggaggc ggacgcgctg cgcgagcagg tgcgcgaggc cctgctgcgc gagccggagg
     1921 gcgagctggc gacggccctc caggcggtcg gcgagcaatt cctggcgatg atcctgacgc
     1981 ccgcgatcgt ctcgctgtgg cgccacacct cggcggaggc ggcccgcttc ccggagatcg
     2041 ggcagatgct gttcgaccgc gggcccggcc tgatgatcga cgggatcgcc gggtacctcc
     2101 agcgctggat cgccaggggc gcgctgcgga tcaccgatgt gcgcgccgcg gcggtccagt
     2161 tcttcatgct gtgccacggc gacctgatgc tgcgctcgca gctcggcatc ttgccgtatc
     2221 cggccgacga tcagatccgc gaggtcgttc gtggggccgt cgcggtgttc gtgcgggcgt
     2281 accagccgcc gcatcaggcc gagagcgccg agcttcaggc gtcgccgtag ccgctgctga
     2341 cccacttgcc ggcagcggcg gccgtcagct tgaagttcgg cgcgacgcgg cgctcgccga
     2401 gcttctcggg gccgtcccag gcggtcagca tggcgtgcgg gtcgtcctcg tcggcggcga
     2461 tgtcgaggtc gacgcgcacg gcggcgccgg catcatcggt gacggtgacc gtgcggtgcc
     2521 gcgcgctgcg gttctgggcc tcgtggcggg tcagcacgtc gcgcgcgccc ttgagcaggg
     2581 tgcgaaagtc cgacacgttg agcggcttgg ggttcttctt gtcgcgcccc atcgtccagt
     2641 gggtcgtcaa gatcggctcg gcgtcgccgt cgcgggtcat cgtcacggcc cagccctcgt
     2701 cgtgctcgtt cttctgcacg cgcgccgtcc agccctccga ggcccaggtc cagccgtcac
     2761 tcgcttcgtt cgccgtgtcg tcctgatcgt cgctcatcgc cgtactcctg cgcccgctcg
     2821 ggctgaacag gtgtatacca cccgaccgcc ccgggcgaaa gtgggccgcg tgagcagcgg
     2881 cacctgtgga gatatcgcgg gccccgcctg aaatttactg cttacaaaag tcgccgccct
     2941 gggcgccgag cgccgggatc gggatcgcct gggtggtgcc gtcgatcgac agcagatacg
     3001 cgacgagctt ctgcacgtcg gtcgggtcga ggaggaagct gttgttcttg gtcagcgcct
     3061 tgcgatgggc gtcgaaggtc ggggacagca gctcctcgag ggtgcgcgcg ttgccggcgt
     3121 ggaagtacgg ggcgccgacg gcgaggccga gcagcgacgg cgggttgtag cccttcgcgt
     3181 cgacggtgcc gccctgcgcg gcggtcatca tgtcggcgcg tagctcggcg acgccgacgg
     3241 cgggcggggc gacgccgaag gtgccgacat tgcggatgat gcactgcagc tggtcgttcg
     3301 ccggcacgcc gaagcgcatg cgctgattca tcggcgtcgt cgcgggcagc agcgcggcgg
     3361 ggaagccggt ggcgagcacg gcggcctcgt cccatacctt gctcttgagc gcctcgttgg
     3421 tcgggccgga cggggtgtag aacacgcgag agatcgtcca cttgtcgccg ccgtggcagc
     3481 cctggcagtt gccgccctgg gcgacatcct taaaaagtgt cgcgccggcg gcgacgagcg
     3541 cggggtcgag gttgctcggc gcacgcggga tgcggatctt ctggatgtag cgcgacag
//
LOCUS       DASWNJ010001229         1634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_58471, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001229 DASWNJ010000000
VERSION     DASWNJ010001229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58471"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1634
                     /locus_tag="VGB85_20720"
     CDS             <1..>1634
                     /locus_tag="VGB85_20720"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEY0136524.1"
                     /translation="PEITKGSCENIRRLLEGTLDFALVQYDVAAEAHRASLSAAPVTT
                     DDQLAGWMCKISPELARRRGLRLVAAISDSAVHMLVRRPVRLDDLDDLESAPIFIGKD
                     GSGSFETARVILGAGGHPLEHLTLFNGSADEALAAMGRRELLMMLRTTGRGHADVQNV
                     LDSGLAALNPLPEDVLNRLIDGYPYYRVCPIEPKHYRGGLEFSLPTVCVSAVLLTVAP
                     PDGVEEAAVQAHGDAVGQLLDSLARVADDPAHHGLGLELRYRDFAEREPIPLHEVAAA
                     RESRSNWTAGLELIGLALAVGLVVFFGRRALRRRGIATGFAFEGQLSNPLVPFAAFAL
                     VVVCSTYLVWWFEHDSNTRLRTLNESFWEMNTFATGNFSAEALKTSGARLVGAVATIL
                     GLGLLAWFTAALTNIFAQDQTRLWRRTRDHFVVLNFREDMLPLIRLLRAPGPARLRSL
                     HVVVPDALPKRVRLQLGKIKALTIHYDNPEAPENLSALRLPRASRVIVLGGEAESRAA
                     SYDPLRIARAVHQACARDPHNLALQGRSHAARAITV"
BASE COUNT          217 a          590 c          587 g          240 t
ORIGIN      
        1 gccggagatc accaagggct cgtgtgagaa catccggcgg ctgctcgagg gcaccctcga
       61 cttcgcgctc gtgcagtacg acgtcgccgc cgaggcccac cgcgcgagcc tcagcgccgc
      121 gcccgtcacc acggacgacc agctcgccgg atggatgtgc aagatctcgc cggagctggc
      181 ccggcgccgg ggccttcggc tggtcgccgc gatcagcgac agcgcggtgc acatgctggt
      241 gcgccggccg gtgcgcctcg acgacctcga cgacctcgag tcggcgccga tcttcatcgg
      301 caaggacggc tcgggcagct tcgagacggc gcgcgtgatc ctcggcgcgg gcgggcatcc
      361 gctcgagcac ctgaccctgt tcaacggcag tgccgacgag gccctcgcgg cgatgggccg
      421 gcgcgagctg ctgatgatgc tacgcaccac cggccgcggc cacgccgacg tgcaaaacgt
      481 gctcgactcg ggcctcgccg cgctcaaccc cctgccggag gacgtgctca accgcctgat
      541 cgacggctat ccgtactatc gcgtgtgccc gatcgagccg aagcactacc gcggcggcct
      601 cgagttcagc ctgccgacgg tgtgtgtcag cgcggtgctg ttgaccgtgg cgccgcctga
      661 cggggtcgag gaggccgcgg tgcaggccca tggcgacgcg gtcgggcagc tgctcgactc
      721 gctggcgcgg gtcgccgacg atccggcgca ccacgggctc gggctcgagc tgcgctatcg
      781 cgacttcgcc gagcgcgagc cgatcccgct gcacgaggtc gccgcggcgc gcgagagccg
      841 gtccaactgg accgcggggc tcgagctgat cggcctcgcg ctggcggtcg ggctggtggt
      901 cttcttcggt cgccgcgcgc tgcgacgccg cgggatcgcc acgggattcg cgttcgaggg
      961 ccagctgtcc aacccgctgg tgccgttcgc cgcgttcgcg ctggtcgtcg tgtgctcgac
     1021 ctacctggtg tggtggttcg agcacgactc gaacacccgc ctgcgtacgc tcaacgagtc
     1081 gttctgggag atgaacacct ttgcgaccgg caacttcagc gccgaggcgc tcaagacctc
     1141 gggcgcgcgg ctggtcggcg cggtggcgac gatcctcggg ctcggcctgc tggcgtggtt
     1201 cacggcggcg ctgaccaaca tcttcgcgca ggaccagacc cggctgtggc ggcgcacgcg
     1261 ggaccacttc gtcgtgctca acttccgcga ggacatgctg ccgctgatcc ggctgctgcg
     1321 cgcgccgggt ccggcgcgcc tgcggtcgct gcacgtggtc gtgcccgacg cgctgcccaa
     1381 gcgggtgcgg ctgcagctcg gcaagatcaa ggcgctgacg atccactacg acaaccccga
     1441 ggcgcccgag aacctgagcg cgctgcggct gccacgggcc tcgcgggtga tcgtgctcgg
     1501 cggcgaggct gagtcgcgcg cggccagcta cgacccgctg cggatcgcac gcgcggtgca
     1561 ccaggcctgc gcccgcgacc cgcacaacct cgcgttgcag ggccgatcgc acgcggcgcg
     1621 ggccatcacg gtcg
//
LOCUS       DASWNJ010001230         1639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_514773, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001230 DASWNJ010000000
VERSION     DASWNJ010001230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514773"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VGB85_20725"
     CDS             complement(<1..337)
                     /locus_tag="VGB85_20725"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0136525.1"
                     /translation="MPRLSHVLVHLSLGAGLALAPACEGKPKQAPPAAEAAKGPPAPA
                     FVGRTIDGEPLELVSLRGQVVLLNVWATWCEPCRKEMPELQALHARHQAQGFTVLGVS
                     VDAARLSAEV"
     gene            complement(409..966)
                     /locus_tag="VGB85_20730"
     CDS             complement(409..966)
                     /locus_tag="VGB85_20730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0136526.1"
                     /translation="MTTACDDFLAQTTPHLPRLFRVGMRLTHQTSEAQDLVQEALMKA
                     WSNWHRYHPGGSLGAWLSRILVNTFISRHRHQRVVDVTAARSDLLDHLFDGGRLDEAQ
                     DPEQAWHGQHLSDEVLAALDALPAHYREVVLLVDLHGLPYRDAADKMHCPLGTVMSRL
                     HRARRLLRDQLTDYARECGLAAAPA"
     gene            complement(1116..>1639)
                     /locus_tag="VGB85_20735"
     CDS             complement(1116..>1639)
                     /locus_tag="VGB85_20735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136527.1"
                     /translation="ALASLGRVDRDHVPLRDQAIAAAAADRDPAMRACAATTARDGST
                     LAGLLRDPSPRVRAFAGFTAAACPVGRARDTEAELARLALADPHPGVARAAAAALVRP
                     RGGEPCGWQLVDAGEANPGDEAGPGWVDLEWREQTLRLPALPLAAHRWLLLPTSEPVS
                     PRRATLASGRTAL"
BASE COUNT          201 a          535 c          693 g          210 t
ORIGIN      
        1 ggacctcggc ggagaggcgc gccgcgtcga cgctgacgcc gagcacggtg aagccctggg
       61 cctgatgacg ggcgtgcagg gcctgcagct cgggcatctc cttgcggcag ggctcgcacc
      121 aggtcgccca cacgttgagc agcacgacct ggccgcgcag gctcacgagc tcgagtggtt
      181 cgccgtcgat cgtgcggccg acgaaggcgg gggcgggcgg gcccttggcg gcctcggctg
      241 cgggcggagc ttgtttgggc ttgccctcgc aggcgggggc cagcgcgaga cctgctccga
      301 gggacaggtg gacgaggacg tgcgagaggc ggggcatgcg ggccggggca gcatagtcgc
      361 tgcgcgggcc gccggcgctg cgccggtgcg tgcagtgggt cacctggttc acgccggggc
      421 ggccgcgagc ccgcactcgc gggcgtagtc ggtcagctgg tcgcgcagga ggcggcgggc
      481 gcggtgcagg cggctcatga cggtgccgag cggacagtgc atcttgtcgg cggcgtcgcg
      541 gtaggggagg ccgtggaggt cgacgagcag cacgacctcg cggtagtggg cgggcagggc
      601 gtcgagggcg gcgagcacct cgtcggagag gtgctgaccg tgccacgcct gctcggggtc
      661 ctgggcctcg tcgaggcggc cgccgtcgaa caggtgatcg agcaggtcgc tgcgggcggc
      721 ggtcacgtcg accacgcgct ggtggcggtg gcgcgagatg aaggtgttga cgaggatccg
      781 cgacagccag gcgccgaggc tgccgcccgg gtgatagcgg tgccagttcg accacgcctt
      841 catcagcgcc tcctggacga ggtcctgcgc ctcgctggtc tggtgcgtca ggcgcatgcc
      901 gacgcggaac agtcgcggga ggtgcggcgt ggtctgggcc aggaagtcat cgcaggcggt
      961 ggtcatgccc ggtctttagc aatctgagac ccatgaaaaa tcatctgcga tctcgcattg
     1021 ttatcgatct cgcgccgggc cgctccgggc gccgggtagc aaagtcggcg gtgatgctac
     1081 gaatcgtggc tggcccggcg cggcccgcga gccgctcaca gcgccgtgcg gccgctcgcc
     1141 agcgtcgcac ggcgcgggct caccggctcg ctcgtcggca gcagcagcca gcgatgggcg
     1201 gccagcggca gggccggcag gcgcaaggtc tgctcgcgcc actcgaggtc gacccagccg
     1261 gggccggcct cgtcgccggg gttcgcctcg ccggcgtcga cgagctgcca gccgcagggt
     1321 tcgccgccgc ggggtcggac cagcgcggcc gctgcggccc gggcgacacc cgggtgcggg
     1381 tcggcgagcg cgaggcgggc cagctcggcc tcggtgtcgc gcgcgcggcc gacggggcag
     1441 gcggcggcgg tgaagccggc gaacgcgcgg acccgcggcg aggggtcgcg cagcagcccg
     1501 gcgagggtcg atccgtcgcg cgccgtggtc gcggcgcagg cccgcatcgc ggggtcgcgg
     1561 tcggcggcgg cggcggcgat cgcctggtcg cgcaggggga cgtgatcgcg gtcgacccgg
     1621 ccgaggctgg cgagggcga
//
LOCUS       DASWNJ010001231         1586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_334399, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001231 DASWNJ010000000
VERSION     DASWNJ010001231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334399"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VGB85_20740"
     CDS             complement(<1..786)
                     /locus_tag="VGB85_20740"
                     /inference="COORDINATES: protein motif:HMM:NF024818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0136528.1"
                     /translation="MTDAEPDDRGELYEEAPCGLILADAEATIVTANRTFVGWLGYSA
                     QELAGRRFQTLLTVPGALLYETHCAPLLRLQGHVGELSLELLARDRSTLPVLLTAVVR
                     TAADGAKRLQIAVFQAPTRREYERELQQARRKAEETAELLHAQAELLAEHSALLIPIQ
                     DGLRVMPVLGTIDGTRGQQMIRALLELDASRVRVVLIDLTGVPSIDAAAAETLRQAAA
                     GVRLRGVRPILTGIRPAVASALVSGGVELQRMLVCGTLQDGIAM"
     gene            complement(783..>1586)
                     /locus_tag="VGB85_20745"
     CDS             complement(783..>1586)
                     /locus_tag="VGB85_20745"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0136529.1"
                     /translation="IVTRAHNVVDAGLPDGRVMLFAHGFGCDQNMWRYVAPAFADTHR
                     IVLFDHVGAGSSDLSAYDPDRYASLHGYADDVIAICDALDLRDVVFVGHSVSAMIGVL
                     AAIEEPQRFRRLVLVAPSPRYIHDDDYVGAFSQQDIDGLLELLESNYMAWSSMLGPVV
                     MANPERPELAEELTASFCRMDPEIARRFARVTFLSDNRADLPKLRVPSLILQCSHDAI
                     APVAIGEYLHARLKNSELVKLDATGHCPNLSAPAETVAAIQAYLERASP"
BASE COUNT          231 a          565 c          571 g          219 t
ORIGIN      
        1 catcgcgatg ccgtcctgca gcgtcccgca cacgagcatg cgctggagct cgacgccgcc
       61 gctcaccagc gcgctggcga ccgcggggcg gatgccggtc aggatcggtc gcacgccacg
      121 caggcgcacg ccggcggcgg cctggcgcag ggtctccgcc gccgccgcgt cgatcgacgg
      181 cacgccggtc aggtcgatca gcaccacgcg cacgcgcgat gcatcgagct cgagcagcgc
      241 gcggatcatc tgctgaccgc gggtcccgtc gatcgtgccg agcaccggca tcacccgcag
      301 gccgtcctgg atcgggatca gcagcgcgga gtgctcggcg agcagctcgg cctgcgcgtg
      361 cagcagctcg gccgtctcct ccgccttgcg ccgcgcttgc tgcagctcgc gctcgtactc
      421 gcggcgcgtc ggcgcctgga acacggcgat ctgcagccgc ttcgctccgt ccgcggcggt
      481 ccgcacaacc gcggtcagca gcaccgggag ggtcgagcgg tcgcgggcga gcagctcgag
      541 cgagagctcg ccgacgtgcc cttgcaggcg cagcagcggc gcgcagtggg tctcgtacag
      601 cagcgccccg gggacggtca gcagcgtctg aaaccgccgc cccgccagct cctgcgcgct
      661 gtacccgagc cacccgacga aggtgcgatt ggcggtcacg atcgtcgcct cggcgtcggc
      721 gaggatcagg ccgcaggggg cctcctcgta cagctcgccg cggtcgtcgg gctcggcgtc
      781 ggtcacgggc tcgctcgctc gaggtacgcc tggatcgccg cgaccgtctc ggccggcgcg
      841 ctcaggttgg ggcaatgtcc ggtcgcgtcg agcttgacca gctcgctgtt cttgaggcgc
      901 gcgtgcaggt actcgccgat cgccaccggc gcgatcgcgt cgtgcgagca ttgcaagatc
      961 agcgagggca cgcgcagctt gggcaggtcg gcccggttgt ccgacaggaa cgtgacgcgc
     1021 gcgaagcgac gcgcgatctc cgggtccatc cggcaaaagc tcgcggtcag ctcctccgcc
     1081 agctcgggcc gctccgggtt cgccatcacc accgggccga gcatgctcga ccaggccatg
     1141 taattgctct cgagcagctc gagcagcccg tcgatgtcct gctgcgagaa cgccccgacg
     1201 tagtcgtcgt cgtggatgta gcggggcgac ggggcgacga gcacgagccg gcgaaaccgc
     1261 tgcggctcct cgatcgccgc gagcacgccg atcatcgcgc tgaccgagtg gccgacgaag
     1321 acgacgtcgc gcaggtcgag cgcgtcgcag atggcgatga cgtcgtcggc gtagccgtgg
     1381 agcgacgcgt agcggtcggg gtcgtaggcg gacaggtcgg acgacccggc gccgacgtgg
     1441 tcgaacagca cgatccggtg ggtgtccgcg aacgcgggag ccacgtagcg ccacatgttc
     1501 tggtcgcacc cgaacccgtg cgcgaacaac atcacgcggc cgtcgggcag acccgcgtcg
     1561 accacgttgt gcgcgcgggt gacaat
//
LOCUS       DASWNJ010001232         1984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_271103, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001232 DASWNJ010000000
VERSION     DASWNJ010001232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271103"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            338..>1984
                     /locus_tag="VGB85_20750"
     CDS             338..>1984
                     /locus_tag="VGB85_20750"
                     /inference="COORDINATES: protein motif:HMM:NF025943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chondroitinase-B domain-containing protein"
                     /protein_id="HEY0136530.1"
                     /translation="MSTSDTSGSTASGDPSSTGTDSPTTGGEPSSTGADTPTTGASTG
                     GGTSTSSGPGQSTGPDDPGCGTTDVGESCGDSSGGEPPPPVCPPGASPPPPTTIVDGV
                     IPSLPWDLAPVTGTEHIVASKDELLAAAAIAAPGDAISIVGGTYDGWGEVVVPASASG
                     TEDAPIVIRSHMEPVTFTGATRLTISGDHVHVYGLSFTGVAAEALKVDGASPAEPAVG
                     VRLSMIHCRETIGQCLLVGDALGTRFDHSLIEKSQSKGVHVGNESRFTRIDHNVFRDR
                     PKPDQNNGFEQIQLGHMRYTGTNDGVDTMVFSRTDALVDHNVFHECKGEGEMIGIKTS
                     GNKIVFNVIADAHDLGRLSFRGGPNNLAYGNVFRDTHGAVRLLGKSNRVLHNLVVDPN
                     QYPGIDLAWGSDMAYQPDNKLYYTVDYIASRQGLIQHNTVAMTAHASSTGRALADDKQ
                     SPDECEIGSKADVIECTVTPFDNTFKANLFVNDDENATVKLSPEMVDMNTYVDNVAAL
                     GPVATLGTTPEMTAALASTFPAPAGDLADIHRALADFVWAP"
BASE COUNT          361 a          731 c          615 g          277 t
ORIGIN      
        1 ctccccaccc acatcgactt gtgcatcgtc ctcatcccgc cctccattcc catcgccatc
       61 atgccagcga tcgacgcgcg ctccggcccc gcgtcacgct cgaatgagcg ccgcaccgtg
      121 accgcgagcg ccgcgcgccg cggcatcgag cgccgaaggc ctgcagatgg tcaggtgcga
      181 gcgcaaccgc gatgacctcc gcggctcacg ctgcttgcct ttgtctggcg gcccgtccga
      241 gtgtgaagtc atgaaatctc cgaacagccc cgcggccaac acgagcgcga tcgcctgctt
      301 tctcctcgcg gcctgtactg ccgagcctcc ctcatcgatg agcacctcgg acacatcagg
      361 atcgacggcg agcggtgacc cgagcagcac cggaacagat tcgccgacga cgggcggcga
      421 accgagcagc acaggcgccg acacgccgac gaccggcgcc tcgaccggag gagggacctc
      481 gacgtcctcc ggacccggac agagcacggg acccgacgat ccgggatgtg gaaccaccga
      541 tgtcggtgag agctgcggcg acagcagcgg cggcgaacca ccgccgcccg tgtgcccgcc
      601 gggtgcgtcc ccgccaccgc ccacgacgat cgtcgacggc gtgatcccct cgctgccgtg
      661 ggacctggcg cccgtcaccg ggaccgagca catcgtcgcc tcgaaggacg agctgctcgc
      721 ggccgccgcg atcgctgccc ccggggacgc catctcgatc gtcggcggca cgtacgacgg
      781 ctggggtgag gtcgtggtgc cggcgagcgc cagcgggacc gaggacgcgc cgatcgtgat
      841 ccgctcacac atggagcccg tcaccttcac gggcgcgacc cgcctcacca tcagcggcga
      901 tcacgtgcac gtgtacggcc tgtcgttcac cggcgtggcg gctgaagcgc tcaaggtcga
      961 cggcgcgagc ccggccgagc cggcggtcgg tgtacgcctg agcatgatcc actgccggga
     1021 gacgatcggg cagtgcctgc tcgtcggcga cgcgctgggc acccgcttcg atcactccct
     1081 catcgagaag tcgcagagca agggggtgca cgtcggcaac gagagcaggt tcacgcgcat
     1141 cgaccacaac gtcttccgcg accgcccgaa gccggaccaa aacaacggat tcgagcagat
     1201 ccagctcggc catatgcgct acaccggcac gaacgacggt gtcgacacca tggtgtttag
     1261 ccgcaccgat gccctcgtcg atcacaacgt ctttcacgag tgcaaaggcg agggggagat
     1321 gatcgggatc aagacctcgg gcaacaagat cgtcttcaac gtgatcgccg acgcgcacga
     1381 tctcggtcgc ctgtcgttcc gcggcggccc gaacaacctc gcctacggca acgtctttcg
     1441 cgacacgcac ggcgccgtgc gcctgctcgg caaatcgaac cgcgtcctgc acaacctcgt
     1501 cgtcgacccg aaccagtacc cgggcatcga cctggcgtgg ggctccgaca tggcgtacca
     1561 gccggacaac aagctgtact acaccgtcga ttacatcgcg tcgagacagg gcctgatcca
     1621 gcacaacacc gtcgccatga ccgcccacgc ctcgtcgacc gggcgcgcgc tcgccgacga
     1681 caagcagtcg cccgacgagt gcgagatcgg gtcgaaggcc gacgtgatcg agtgcacggt
     1741 cacgccgttc gacaacacct tcaaggccaa cctcttcgtc aacgacgacg agaacgcgac
     1801 cgtgaagctg tcgccggaga tggtggacat gaacacctat gtcgacaacg tcgcggcgct
     1861 cggccccgtc gccaccctgg ggacgacacc cgagatgacc gcggcgctcg cgtcgacctt
     1921 ccctgcgcct gccggcgacc tcgccgacat tcaccgcgcg ctcgccgact tcgtctgggc
     1981 tccg
//
LOCUS       DASWNJ010001233         1572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_92235, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001233 DASWNJ010000000
VERSION     DASWNJ010001233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92235"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VGB85_20755"
     CDS             <1..346
                     /locus_tag="VGB85_20755"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY0136531.1"
                     /translation="PLLGGEQFHKGIDFSANTGDPVLAVADGVVSFVGERSGYGNVVE
                     IDHGNGYVTRYAHNSRLGRRVGELVRTGDEIAKAGSTGRSTGAHVHLELWQDGRVVNP
                     RPFLEQGRRLRG"
     gene            433..>1572
                     /locus_tag="VGB85_20760"
     CDS             433..>1572
                     /locus_tag="VGB85_20760"
                     /inference="COORDINATES: protein motif:HMM:NF019156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0136532.1"
                     /translation="MAAYERRHRPFSHRSIMLNSLLTRVFGSRNERLLRQLQRSVEKI
                     NSLEAGLKALSDDELRAKTPEFQQRLANGENIEKLLPEAFAVCREASIRVLGMRHYDV
                     QLIGGMVLHLGKIAEMRTGEGKTLVGTLPVYLNALAGKGVHVITVNDYLARRDAAWMG
                     KLYSWLGLSVGVVYPGMPHADKHAAYASDITYGTNNEFGFDYLRDNMALSKTDRVQRG
                     LHYAIVDEVDSILIDEARTPLIISGPADESPELYIKVNRIVPQLARQEKEDSDGDYWV
                     DEKGKQVHMSEAGQEHAEALLRAAGIIGEDDDLYDPKNLSVVHHLNAAMRAHAIYQRD
                     VDYIVRDGEVVIVDEFTGRTLPGRRWSDGLHQAVEAKEGVPVQREN"
BASE COUNT          280 a          542 c          515 g          235 t
ORIGIN      
        1 tccgctgctc ggcggcgagc agttccacaa gggcatcgat ttctcggcca ataccggcga
       61 cccggtgctc gcggtcgccg acggcgtggt gagcttcgtc ggcgagcgct cgggctacgg
      121 caacgtcgtc gagatcgacc acggcaacgg ctacgtgacg cgctatgcgc acaactcgcg
      181 gctcggccgc cgggtgggcg agctggtgcg tacgggcgac gagatcgcca aggccggctc
      241 gaccggccgc tcgacggggg cccatgtgca tctggagctc tggcaggacg gtcgcgtcgt
      301 caatccgcgc ccgttcctcg agcagggccg ccgcctgcgc ggttgaggca tcgggaccgg
      361 cttgcaaacg ccggtcccag ccaccagcta caatccgcga tgccgtaaaa gggcgcccag
      421 cgcccttttt tattggcggc gtacgagcgc cgccaccgtc ccttttccca ccggtccatc
      481 atgctcaaca gcctgctcac ccgcgttttc ggcagccgta acgaacgcct gctccgtcag
      541 ttgcagcggt cggtcgaaaa gatcaacagc ctcgaggccg gcctcaaggc gctcagcgac
      601 gatgaactca gggccaagac gccggagttc cagcagcgcc tggcgaacgg cgagaacatc
      661 gagaagctgc tgcccgaagc ctttgccgtc tgccgcgagg cgtccatccg cgtgctcggc
      721 atgcgccact acgacgtgca gctgatcggc ggcatggtgc tgcacctggg caagatcgcg
      781 gagatgcgca ccggcgaggg caagaccctg gtcggcacgc tgccggtcta cctcaacgcg
      841 ctcgccggca agggcgtgca cgtgatcacc gtgaacgatt acctggcgcg tcgcgacgcc
      901 gcctggatgg gcaagctcta cagctggctc ggcctgtcgg tcggcgtggt gtatccgggc
      961 atgccgcatg cggacaagca tgccgcctac gcctccgaca tcacctacgg caccaacaac
     1021 gagttcggct tcgactacct gcgcgacaac atggcgctgt cgaagaccga ccgcgtgcag
     1081 cgtggcctgc attacgcgat cgtcgacgag gtcgactcga tcctgatcga cgaggcgcgc
     1141 accccgctca tcatctccgg ccccgccgac gaatcgcccg agctctacat caaggtcaac
     1201 cgcatcgtcc cgcagctcgc gcggcaggag aaggaagaca gcgacggcga ctactgggtc
     1261 gacgagaagg gcaagcaggt ccacatgtcc gaggccggtc aggagcatgc cgaggcgctg
     1321 ctccgcgcgg cgggcatcat cggcgaggac gacgacctct acgatccgaa gaacctcagc
     1381 gtcgtgcatc acctcaatgc ggcgatgcgc gcgcatgcga tctaccagcg cgacgtggac
     1441 tacatcgtcc gcgacggcga ggtggtcatc gtcgacgagt tcaccggccg cacgctgccg
     1501 ggccgccgct ggtccgacgg cctgcaccaa gcggtcgagg cgaaggaggg cgtgcccgtc
     1561 cagcgcgaga ac
//
LOCUS       DASWNJ010001234        12453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_587633, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001234 DASWNJ010000000
VERSION     DASWNJ010001234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 12453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12453
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587633"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VGB85_20765"
     CDS             <1..253
                     /locus_tag="VGB85_20765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136533.1"
                     /translation="GYRTKGLWHRANLGRYVGALDLVDFMDELTAPRPRKQVMRTTEK
                     IFNAVRGRALDLLADLGETASPAERTLPLLDFLLETPIA"
     gene            336..1571
                     /locus_tag="VGB85_20770"
     CDS             336..1571
                     /locus_tag="VGB85_20770"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEY0136534.1"
                     /translation="MTPRRVVITGAGVASPIGNSLAEVSLSLQTDRHGIVTMPEWDRI
                     GDLRTRLAGVVQNLDLDKRWNRKQTRTMGRVALLATYATEQALAMAGLTGDEVIGSGH
                     AGLAYGSTSGSSEALAEFCTALFFNMNMRGLQSTSFLKFMAHTCAANLAQFFGVRGRI
                     VPTNSACTSGAQAIGYAYEAIKYGMQDVMIAGGADEMHYTTAVTFDLMFATSANYNAQ
                     PELSPRPFDVKRDGLVVGEGAGTLILESLERAQARGANILGELRGFGTNCDGIHITAP
                     SEPGMRAAMVLSMRDAGVDPADIDYINAHGTATDIGDIAESQATRAVFERAAPISSSK
                     SFTGHTMGACGAIEAVYCLAMLQGGFMAPTRNLELVDPRCAELDYVRELRRAELRTIM
                     SNNFAFGGVNTSMIFGRHE"
     gene            1592..2311
                     /locus_tag="VGB85_20775"
     CDS             1592..2311
                     /locus_tag="VGB85_20775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136535.1"
                     /translation="MLVRLSLAVALLLGVVLAPTSAPQASSCQKAGDCPGGLRCHQGS
                     CVAEPIRCAAACAARDLRGECFGYTADFCGPDAVCARACVHRSMYGDCTTWAADACGE
                     NMACAIRCEQRATDGTCLGYDADVCETRAACTTNCTQRASNGACVAYGGDLCGPSYAC
                     APRCLQRGLGGDCTEWGQDVCGPHAACAKRCTARDGAGNCNAWGGDVCGEGAVCVKHC
                     ISRGTFGACLAYGPDLCEAGN"
     gene            complement(2343..3074)
                     /locus_tag="VGB85_20780"
     CDS             complement(2343..3074)
                     /locus_tag="VGB85_20780"
                     /inference="COORDINATES: protein motif:HMM:NF016629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HEY0136536.1"
                     /translation="MTGTPHDALFKATFSQVVYAAEELRYVLPPELVAVIDFSTMQVE
                     PGSFVDEALRARYTDLLYSLAIAGQDARIYVLFEHQSSGERWMALHERAASACVRLVL
                     SALQQARTGTEVRKLMFGWVDLIQALLREPNGHSALGLIVRYLLEVRGAGELGVIDTT
                     LREITADNGEVMQTIAQMLESRGMERGLQQGRQEVVLRLLRRRFGELPAAVTQRVTAA
                     GTDELDLWADRLLFAGDLDEVFAAP"
     gene            3353..4570
                     /locus_tag="VGB85_20785"
     CDS             3353..4570
                     /locus_tag="VGB85_20785"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0136537.1"
                     /translation="MPDPHPDALVPRLGDRSLFPTLEPAAYLNHAAISPHSLAVQRAI
                     TAVTADYARRGMGAFLTWDAERRRLRGQLARLLGAEVDDIALISNTTRGVTDIALCLP
                     WRQGDRIVLFEGEFPANVTPWQRAAELFGLEVEMLPLRDFLHDHGHGESQGLRSLREA
                     LRQPTRLVAVSAVQFQSGLRMPVPEIAALCHEAGAELFVDAIQALGVCPIDVVASGVD
                     YLAAGGHKWMMGCEGLGCLYIRRDRVEALQPRVAGWLSHEDGLGFLLAGAGLLRYDRP
                     IRRRADFVEGGAYNTIGCAALAASLDLILTLGVPAIFEHVQHYIDELEDGLCFRGFTS
                     LRARKTAERSGILSVLPPNPGDVIGLQRELVERGVSCSTPDGVLRFSPHWPNALAEIP
                     GVLAAVDAALGNP"
     gene            4633..6372
                     /locus_tag="VGB85_20790"
     CDS             4633..6372
                     /locus_tag="VGB85_20790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136538.1"
                     /translation="MDRQRPRTTSDEIDLYIRTYYSLLRSSGEVRVRALEESHSFSDS
                     SLHANALSGTPDVAAFAYSAARLPACMPKIHRIVLGQSHESFEAAGFAIRTWERVQTR
                     GRRRPLRFDGGDLLGAFISSTSDIDDIVPIVTAYQIVWNQMHLLLADSELGRRLRAAP
                     PEGEPPKVDCAELGPLLGIDDDAARTLCQALDKDWVNSLREISMRTCDLSLRVLSGSY
                     SQYQRASQRWWSGIEPGYLRETDPRRPPIYFVSSNTHSLLNMLGGYARSHRDAIVEYA
                     KRRNPENLAKPLLEAIAGGREPEICNITYYLLRAFLHDDPSQTQNRLAQIQAYESESG
                     ITSIDVPGKIDVNAQIFQLSSLIPDRLDPRLDMPGLERLKRSDAVIINIDYPLGMAAY
                     HHLARIGQASGEIRGLYIMGKAASLNARVGDIMISTVVQDEHSRNTYLFRNCFAAADV
                     QPYLKFASVFDQQKALTVRGAFLQNREYMSVFYREGYTVLEMEAGPYLSAVYELVNPQ
                     RHPNDEIVHLSVNTPFDVGILHYASDTPYSRRQSLLSKSLSFFGVEATSACAVAICRQ
                     ILSREIARLGDCP"
     gene            6369..7346
                     /locus_tag="VGB85_20795"
     CDS             6369..7346
                     /locus_tag="VGB85_20795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136539.1"
                     /translation="MTTMHEPSPWDVIAGPAPAGEPSQLLRHALHFALTAPSPGNSQP
                     WRFMLDELEVLVFTDPARSLPVRDPEGQQRIVAGGAAIGLLRVALRALGLGEQTTLLP
                     GDHPDLLARITLTGSVAPIPEETWLMQAAPKRRTHRGPFADRPVRERLQARLQAMARD
                     AGAELTLLHTRAQREAYAARVEAALELSESDPAAQAERAAWPEPGPEPFESPTGGPAR
                     GPEIAAGTPLLALITTETDDPRAWLIAGQALARVLLRGRVDHLQASYLQGPLARGEDR
                     FAVAEQAAVLGDLTQTPGYAQLALRLGGGAEQPASSRRPLSEVLLDAVP"
     gene            7407..8609
                     /locus_tag="VGB85_20800"
     CDS             7407..8609
                     /locus_tag="VGB85_20800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136540.1"
                     /translation="MLRLMAPLVALLVTGCGEAPPATPPQPAKTPATPAAPPATPPAA
                     PETAPTDEQRCGPAHTYLDAVRSDPAAVTDPLAAMRTAYQGTALQDLVKRSDAATARQ
                     DDAAISTALAGTGTDAAVAAQYAIHGAIGQWMRHNLKVAAEGKGASERAFAWTEARCV
                     WTRHLRHLGVPLVARATEAARDDATVLEVIDLAFAGGAGAVTAEPIDERVLLRARQTV
                     EKNWYRLVHRELAHHATLARSGDPLAARRALGLFDMLRDRMPDKNSPGITIVTAMLSG
                     PAASIDPAAVMREIDVALVKRARKYCSEAVDPKFVGTAAGLASVAEGAAYTRVLMPGL
                     LKLPEFDATAHMAAWQAFSEAVGAGESPDELRRLSDELVHWNCAYQQALGIRECTATA
                     DEVAAKPK"
     gene            complement(8613..9158)
                     /locus_tag="VGB85_20805"
     CDS             complement(8613..9158)
                     /locus_tag="VGB85_20805"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY0136541.1"
                     /translation="MTAAQHFARILVPIDFQPATDEQIAAGQAVLVEKQYIDYAPASL
                     RSISLAAGLASLTGGSIILLHVTPPLAYSSMYTGSLTVGLSQQVMAEVHKAAHDTSIA
                     ALQVVAQRHCPGIPVELSARPGVPLSVILGEAERHAVDLIVLAASGRSRVARFFVGST
                     ADRIIREANCPVLVVPAQPST"
     gene            9216..10559
                     /locus_tag="VGB85_20810"
     CDS             9216..10559
                     /locus_tag="VGB85_20810"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0136542.1"
                     /translation="MRLDVPGPDPLPIATLRFARDPYRYFSSCRRRLGPVFQSWLAGN
                     RIVLSGEPEHVRTIFAADHDTAGAFNPGHLEALLGKHSLILIDGARHQSERKLLSPPL
                     HGARMRSYAGIVRESALHRAAAWRVGAPLVMQDATQALSLDVIVKAVFGVQEPARVAA
                     FEAALLATMSSVWMPAVLIRGLQRDLGPLTPWRRFARARDHADALIREEIAVRRRAPP
                     GDDILSLMISARYDDGEGMRDDQIRDELMTLLAAGHETTAIALAWAMYWLHRHPDTLE
                     RLRAELALAGDDLDAIASLPYLDAVCHETLRLYPIVPLAPRTLRRPLTLGPHTLPPGT
                     HVGACIALLHRHPDLYPEPDEFRPARWLARKFTPWEFAVFGGGIRRCIGAAFALYAMK
                     HALAALLPRHRFRLLEDRPVTPVRRNITLGPRGGIRMVLTERTGQPPAPAGAHVS"
     gene            10635..11792
                     /locus_tag="VGB85_20815"
     CDS             10635..11792
                     /locus_tag="VGB85_20815"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY0136543.1"
                     /translation="MPDSASLVRTDASPDALELRPVDGFPRWLGTRLARFFLRVVSME
                     ENDAAKLARMVTPTSGYFRRDRHVHPAVDPTTYSVKFTGVAAPRSFSLADLQALPRET
                     RVCVQECAGNGNHIMGSAGLCGQAVWSGPTFESLLELCGGPGDATHFAFHGLDDLGLV
                     KRGYHYGLSLDELRRAQAIVALTMNGEPLTRRHGFPARLIVPRIYSMSHVKWLGHVEG
                     KTRPHMGIHNRFVFTNKERRDGKWVRVQARWIGLKSVIAHCTRDGSGWRLTGCAWGGE
                     RPIAGVEVSTDGGLSWQPARWQTPIEYFNEHPELATPEPEALAGAWGVFTATWLPSGP
                     GTYKVGCRAIDVDGNIQQLDNDPNVHGHFNQTRVKWRMVTVPERRLGPAGS"
     gene            complement(11807..>12453)
                     /locus_tag="VGB85_20820"
     CDS             complement(11807..>12453)
                     /locus_tag="VGB85_20820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ribulose phosphate epimerase"
                     /protein_id="HEY0136544.1"
                     /translation="PATEKCMPYANDGGGSWNATKCTPLDASPGQPGDECQAEGDGTS
                     GVDNCALGTMCWFLDAENKGTCIEMCTGSADAPMCPGGKTCDESNDGVIIICLDTCDP
                     LAQTCPDGQICFFDGVDAFICDFDASGEQGKYGDPCEYINVCDYGLFCADVTLVPGCA
                     ETMATGCCSPYCNVNDPNTCPGKDAMPGQECVPWYGEDTPPPGQEDIGACAIVL"
BASE COUNT         1769 a         4844 c         4075 g         1765 t
ORIGIN      
        1 gggctaccgc accaagggtc tgtggcaccg ggccaacctc gggcgctacg tcggcgcgct
       61 cgacctcgtc gacttcatgg acgagctgac ggcgcctcgc ccgcgcaagc aggtcatgcg
      121 caccaccgag aagatcttca acgccgtgcg tgggcgggcg ctcgacctgc tcgccgacct
      181 cggcgagacg gccagcccgg cggagcgcac gctcccgctg ctggacttcc tgctggagac
      241 cccgatcgcc tgagcgagtg gccttacgca cgtcgcctgg acgtgccgac gctcgctggc
      301 taccatctcc cgttgctcac gaaacgcacc gatccatgac tccacgtcgc gtcgtcatca
      361 ccggggccgg cgtcgcctcg ccgatcggga actcgctcgc cgaggtcagc ctgtccttgc
      421 agacagaccg ccacggcatc gtcaccatgc ccgagtggga ccgcatcggc gacctgcgca
      481 cgcggctcgc cggcgtcgtc cagaacctcg acctcgacaa gcgctggaac cgcaagcaga
      541 cgcgcacgat gggccgcgtc gcgctgctcg ccacctacgc gaccgagcag gcgctggcga
      601 tggccggcct gaccggcgac gaggtgatcg gctcgggcca cgcggggctc gcctacgggt
      661 cgacctcggg ctcgtcggag gcgctcgccg agttctgcac cgcgctgttc ttcaacatga
      721 acatgcgggg cctgcagagc acgagcttcc tcaagttcat ggcgcacacc tgcgcggcca
      781 acctggcgca gttcttcggg gtgcgcggtc gcatcgtgcc gaccaactcg gcgtgcacca
      841 gcggcgccca ggcgatcggc tacgcctacg aggcgatcaa gtacggcatg caggacgtca
      901 tgatcgccgg cggcgcggac gagatgcact acaccaccgc cgtcaccttc gacctgatgt
      961 tcgccacctc ggcgaactac aacgcccagc cggagctgag cccgcgcccc ttcgacgtca
     1021 agcgcgacgg cctggtcgtc ggcgagggcg ccggcacgct gatcctcgag agcctcgagc
     1081 gcgcgcaggc ccgcggggcc aacatcctcg gagagcttcg cggcttcggc accaactgcg
     1141 acggcatcca catcaccgcc ccgtccgagc ccggcatgcg cgccgcgatg gtgctgtcga
     1201 tgcgcgacgc cggggtcgac cccgccgaca tcgactacat caacgctcac ggcacggcga
     1261 cggacatcgg cgacatcgcc gagagccagg cgacccgcgc ggtgttcgag cgcgcggccc
     1321 cgatctcgtc gtccaagagc ttcaccggcc acacgatggg cgcctgtggc gcgatcgaag
     1381 cggtctactg cctggcgatg ctccagggcg gcttcatggc cccgacccgc aacctggagt
     1441 tggtcgaccc gcgctgcgcc gagctcgact acgtccgcga gctgcgacgc gccgagctgc
     1501 gcacgatcat gagcaacaac ttcgcgttcg gcggggtcaa cacctcgatg atcttcggcc
     1561 gccacgagtg agccctgcta cgctgacggc cgtgctggtc cgcctgtccc tggccgtcgc
     1621 gctgctgctc ggcgtcgtgc tcgcgccgac gagcgcgccg caggcgagca gctgccaaaa
     1681 ggcgggtgac tgcccgggcg gcctgcgctg ccaccaggga tcttgcgtcg ccgagccgat
     1741 ccgctgcgcg gccgcctgcg ccgcccggga cctgcgtggc gagtgttttg gttataccgc
     1801 cgacttctgc gggcccgatg ccgtgtgcgc gcgcgcctgc gtgcaccgca gcatgtacgg
     1861 cgactgcacg acctgggccg ccgacgcctg cggcgagaac atggcctgcg ccatccgctg
     1921 cgagcagcgc gcgaccgacg gcacctgcct cggctacgac gccgacgtgt gcgagacccg
     1981 ggccgcctgc accacgaact gcacccagcg cgccagcaac ggcgcgtgcg tggcctacgg
     2041 cggcgacctg tgcggcccgt cgtacgcctg cgcgccgcgc tgcctgcagc gcggtctcgg
     2101 cggcgactgc acggagtggg gccaggacgt ctgcggcccg cacgcggcct gcgccaagcg
     2161 ctgcaccgcc cgcgacggcg ccggcaactg caacgcctgg ggcggcgacg tgtgcggcga
     2221 gggggcggtc tgcgtcaagc actgcatcag ccgcggcacc ttcggcgcct gtctggccta
     2281 cggcccggac ctctgcgagg ccggcaactg agcggtgctc ggggcggcca gggccgcggc
     2341 cctcacgggg cggcgaagac ctcgtcgagg tcgccggcga acagcagccg gtcggcccag
     2401 agatcgagct cgtcggtccc ggcggcggtg actcgctggg tgaccgccgc cggcagctcg
     2461 ccgaagcgcc gtcgcagcag ccgcagcacc acctcctggc ggccctgctg gagcccacgc
     2521 tccattcccc tcgactccag catctgcgcg atggtctgca tgacttctcc gttgtcggcg
     2581 gtgatctcgc gaagggtcgt gtcgatgacg ccgagctccc cggcgccacg gacctcgaga
     2641 agataccgca cgatcagccc gagcgcactg tggccgttcg gctcgcgcag cagggcctgg
     2701 atcaggtcga cccagccgaa catcagcttg cggacctccg tgccggtgcg cgcctgctgc
     2761 agcgccgaca gcaccaggcg gacgcaggcc gaggccgccc gctcgtgcag cgccatccac
     2821 cgctcgccgc tgctctggtg ctcgaacagc acgtagatgc gagcgtcctg ccccgcgatc
     2881 gcgagtgagt agagcaggtc ggtgtagcga gcgcgcagcg cctcgtcgac gaagctgccg
     2941 ggctcgacct gcatcgtcga gaagtcgatc accgcgacga gctcgggcgg cagcacgtac
     3001 cgcagctcct cggccgcgta cacgacctgc gaaaaggtgg ccttgaacag ggcgtcgtgc
     3061 ggcgtaccgg tcacgccgcc gcacgctagc gcgccacgat gccgatgcaa ggttcgcggt
     3121 cgcaaaactt cttgagtgac cggcgatcgc cgttgggtcg agtcgcctcg acaacaccgt
     3181 gtggagcacg gtctattgac tcacatcgcc ggcggtctcg accctccaaa acctccgcgc
     3241 cgaccgctac cggccgtccc catgcagcgc tgccccccgc ctccctgcat cttcaccggc
     3301 aatgcacgcc gggcgagcgc gtcgctgcgg ccccgtgcta gcctgcgccg ccatgcccga
     3361 tccgcacccg gacgcgctgg tcccccgcct cggggaccgc agcctgtttc cgacgctcga
     3421 gcccgccgcg tacctcaatc acgccgccat ctcgccgcac tcgctcgccg tgcaacgagc
     3481 gatcaccgcc gtcaccgccg actacgcccg ccgcggcatg ggggccttcc tgacctggga
     3541 cgccgagcgc cggcgcctgc ggggccagct cgcgcgcttg ctcggcgccg aggtcgacga
     3601 catcgccctg atctccaaca ccacccgcgg cgtcacggac atcgccctgt gccttccgtg
     3661 gcgccagggt gaccgcatcg tgctgttcga gggcgagttc ccggccaacg tcaccccctg
     3721 gcagcgcgct gcggagctgt tcggcctcga ggtcgagatg ttgccgctac gcgacttctt
     3781 gcacgaccac gggcacggcg agagtcaggg cctccggagc ctgcgcgagg ccctgcgcca
     3841 gccgacgcgc ctcgtcgcgg tctcggcggt gcagttccag agcggcctgc gcatgcccgt
     3901 ccccgagatc gccgcgctgt gtcacgaggc cggcgccgag ctgttcgtcg acgcgatcca
     3961 ggcgctcggc gtgtgtccga tcgacgtcgt cgccagcggc gtcgactatc tcgcggccgg
     4021 cggccacaag tggatgatgg gctgcgaggg cctcggttgc ctgtacatcc gccgggaccg
     4081 cgtcgaggcc ctgcagccgc gcgtcgccgg ctggctcagc cacgaggacg gcctcggctt
     4141 cctgctcgcc ggcgccggcc tgctgcgcta cgaccggccg atccgccggc gcgccgactt
     4201 cgtcgagggt ggcgcctaca acacgatcgg ctgcgccgcg ctcgcggcct cgctcgacct
     4261 gatcctcacg ctcggtgtcc cggcgatctt cgagcacgtg cagcactaca tcgacgagct
     4321 cgaggacggc ctgtgcttcc gcggcttcac cagcctgcgc gcgcgcaaga ccgccgagcg
     4381 ctcgggcatc ctgtcggtgc tgccgccgaa ccccggcgac gtcatcggcc tgcagcgcga
     4441 gctcgtcgag cgcggcgtgt catgttccac gcccgacggc gtgctgcgct tctcgcccca
     4501 ctggcccaac gccctcgccg agatccccgg cgtgctcgcg gccgtcgacg ccgctctcgg
     4561 caacccctga tcttcccatc gctccgctcg cttcctcatc gctccctcat cgctccctca
     4621 tcgcgagacc ccatggaccg ccagcgcccc cgtacgacca gcgacgagat cgacctctac
     4681 atccgcacct actactcgct gctgcgcagc agcggcgagg tgcgcgtgcg cgcgctcgag
     4741 gagtcccact ccttcagcga ctcgagcctg cacgccaacg cgctctcggg cacgccggac
     4801 gtcgccgcct tcgcctacag cgccgcgcgc ctgccggcgt gcatgccgaa gatccaccgc
     4861 atcgtgctcg gtcagtcgca cgagagcttc gaggccgccg gctttgcgat ccgcacctgg
     4921 gagcgcgtgc agacccgcgg ccgccgtcgc ccgctgcgct tcgacggcgg cgacctgctc
     4981 ggcgccttca tctcgagcac cagcgacatc gacgacatcg tcccgatcgt caccgcctac
     5041 cagatcgtct ggaaccagat gcacctgctg ctcgccgaca gcgagctcgg tcggcgcctg
     5101 cgggccgcgc cgcccgaggg tgagcccccg aaggtcgact gcgccgagct cggcccgctg
     5161 ctcggcatcg acgacgacgc cgcgcgcacg ctgtgccagg cgctcgacaa ggattgggtc
     5221 aacagcctgc gcgagatctc gatgcgcacc tgcgacctct cgctgcgcgt gctcagcggc
     5281 tcctactcgc agtatcagcg ggcctcgcag cgctggtggt cgggcatcga gcccggctac
     5341 ttgcgagaga ccgacccgcg ccgcccgccg atctacttcg tgtcctcgaa cacgcactcg
     5401 ctgctcaaca tgctcggcgg ctacgctcgc tcgcaccgcg acgcgatcgt cgagtatgcc
     5461 aagcggcgca acccggagaa cctcgccaag cccttgctgg aggcgatcgc cggcggccgc
     5521 gagcccgaga tctgcaacat cacgtattac ctgctgcgcg ccttcctgca cgacgacccc
     5581 tcgcagaccc agaaccgcct ggcccagatc caggcctacg agtccgagag cgggatcacc
     5641 tcgatcgacg tgcccggcaa gatcgacgta aatgcccaga tctttcagct ctcgagcctg
     5701 atccccgacc gcctcgaccc gcgcctcgac atgccgggcc tcgagcgcct caagcgcagc
     5761 gacgcggtga tcatcaacat cgactacccg ctcggcatgg ccgcctatca ccacctcgcg
     5821 cgcatcggcc aggccagcgg cgagatccgc gggctctaca tcatgggcaa ggccgcgagc
     5881 ctcaacgccc gcgtcggcga catcatgatc tcgacggtcg tgcaggacga gcactcgcgc
     5941 aacacctacc tgtttcgcaa ctgcttcgcc gccgcggacg tccagcccta cctcaagttc
     6001 gcctccgtct tcgaccagca gaaggcgctg accgtccgcg gcgcgttcct gcagaaccgc
     6061 gagtacatga gcgtgttcta ccgcgagggt tacaccgtgc tcgagatgga ggccggcccc
     6121 tatctctccg cggtgtacga gctggtcaac ccccagcgcc accccaacga cgagatcgtg
     6181 cacctctcgg tcaacacgcc cttcgacgtc ggcatcctcc actacgcctc ggacacgccc
     6241 tactcgcggc gccagagcct gctgtccaag agcctgtcgt tcttcggcgt cgaggcgacc
     6301 agcgcctgcg cggtcgccat ctgccggcag atcctgtcgc gggagatcgc ccgcctcggg
     6361 gactgcccgt gaccacgatg catgagccga gcccctggga cgtgatcgcc gggccggcgc
     6421 cggccggcga gccttcacag ctgctgcgcc acgcgctgca cttcgccctg accgcgccct
     6481 cgcccggcaa ctcgcaaccg tggcgcttca tgctcgacga gctcgaggtc ctggtgttca
     6541 ccgacccggc gcgcagcctc ccggtccgcg acccggaggg ccagcagcgc atcgtcgccg
     6601 gcggcgccgc gatcgggctg ctgcgcgtcg ccctgcgcgc gctcggcctc ggcgaacaaa
     6661 caaccctgct gcccggcgac caccccgacc tgctcgcccg catcaccctc accggctcgg
     6721 tcgcgccgat acccgaggag acgtggctga tgcaggcggc ccccaagcgc cgcacgcatc
     6781 gcgggccgtt cgccgaccgc ccggtccgtg agcgcctgca agcgcgccta caggcgatgg
     6841 cccgcgacgc gggcgccgag ctgacgctgc tgcacacccg cgcgcagcgt gaggcctacg
     6901 ccgcccgcgt cgaggccgcg ctcgagctct ccgagtccga ccctgccgcg caggccgagc
     6961 gcgccgcctg gcccgagccc ggcccggagc ccttcgaatc accgaccggc ggccccgcgc
     7021 gcggcccgga gatcgccgcc ggcacgcccc tgctcgcgct gatcaccacc gagaccgacg
     7081 acccgcgcgc gtggctgatc gccggccagg cgctcgcgcg cgtgctgctg cgcggccgcg
     7141 tcgaccacct ccaggcctcg tacctgcaag ggccgctcgc gcgcggggag gaccgcttcg
     7201 cggtcgccga gcaggcggct gtcctcgggg acctgaccca aacgccaggt tacgcccagc
     7261 tcgcgctgcg cctcggcggc ggcgccgagc agcccgcgtc ctcgcgccgc ccgctgtccg
     7321 aggtcctcct cgacgccgtg ccatgagccc gatgcccgga tagatccccc gctcgggctg
     7381 cacggcgctg cgtgataggc tggggcatgc tccgcctcat ggcaccactg gtggccctgc
     7441 tcgtgaccgg ctgcggcgag gccccgcctg ccacaccgcc ccagcccgcg aagacccccg
     7501 cgacgccggc cgccccaccc gcgaccccgc ccgcggcccc cgagaccgcg cccaccgacg
     7561 agcagcgctg cggcccggcc cacacctacc tcgacgccgt gcgcagcgac cccgccgcgg
     7621 tcaccgaccc gctcgcggcc atgcgcaccg cctatcaggg caccgccctg caggatctgg
     7681 tgaagcgcag cgacgccgcc accgcgcgcc aggacgacgc agcgatctcc accgcgctcg
     7741 cgggcactgg cacggacgcc gcggtcgcgg cccagtacgc gatccacggc gcgatcgggc
     7801 agtggatgcg ccacaacctc aaggtcgccg ccgagggcaa gggcgcctcc gagcgcgcat
     7861 tcgcgtggac cgaggcgcgc tgcgtgtgga ccaggcacct gcgccacctc ggcgtaccac
     7921 tggtcgcgcg ggccaccgag gccgcccgcg acgacgccac cgtgctcgag gtcatcgacc
     7981 tcgcgttcgc cggcggggcc ggggccgtga ccgccgagcc gatcgacgag cgcgtgctct
     8041 tgcgtgcccg ccagacggtc gagaagaact ggtaccgcct ggtccaccgc gagctcgcgc
     8101 atcacgccac gctcgcgcgc agcggcgacc cgctcgcggc ccgccgcgcg ctgggcctgt
     8161 tcgacatgct gcgcgaccgc atgcccgaca agaacagccc cggcataacg atcgtcaccg
     8221 ccatgctctc gggccccgcc gcgagcatcg accccgccgc cgtcatgcgc gagatcgacg
     8281 tcgccctcgt caagcgcgcc cgcaagtact gcagcgaggc cgtcgacccc aagttcgtcg
     8341 gcaccgccgc cggcctcgcc agcgtcgccg agggcgcggc ctacacccgc gtgctgatgc
     8401 ccggcctcct caagctcccc gagttcgacg cgaccgccca catggccgcc tggcaggcgt
     8461 tctccgaggc cgtcggcgcc ggcgagagcc ccgacgagct caggcgcctc agcgacgagc
     8521 tcgtgcactg gaactgcgcg taccagcagg cgctcgggat ccgcgagtgc accgcgaccg
     8581 ccgacgaggt cgcggcgaag ccgaaatgac ttctacgtgc tcggctgggc cgggaccacg
     8641 agcaccgggc agttggcctc gcggatgatg cggtcggccg tgctgccgac gaagaagcgg
     8701 gcgacgcggc tgcggccgct cgccgcgagc acgatgaggt cgaccgcgtg gcgctcggcc
     8761 tcgccgagga tcacgctcag cggcacgccg gggcgcgccg acagctcgac cgggatgccc
     8821 gggcagtggc gctgcgcgac cacctgcagc gccgcgatcg acgtgtcgtg ggcggccttg
     8881 tggacctcgg ccatcacctg ctgcgacaaa ccaaccgtca ggctgccggt gtacatcgag
     8941 ctgtaggcca gcggcggcgt cacgtgcagc aagatgatgc tgccgccggt caggctcgcc
     9001 aggcccgcgg cgagcgagat gctgcgcagc gacgccgggg cgtagtcgat gtactgcttc
     9061 tcgaccagca ccgcctggcc ggcggcgatc tgctcgtcgg tcgccggctg aaagtcgatc
     9121 gggaccaaaa tgcgggcgaa gtgctgggcg gcggtcatcg tcgtcagcat agcaaagccg
     9181 cgcagccggc cggaagtcca ggtacaaaac tccgcatgcg cctcgacgtg cctggccccg
     9241 acccgctgcc gatcgccacc ttgcggtttg cgcgggaccc gtaccggtat ttctcctcat
     9301 gtcgtcggcg gctcggcccg gtgtttcaga gctggctcgc cggcaatcgc atcgtcctca
     9361 gcggcgaacc cgagcacgtg cggacgatct tcgccgccga tcacgacacc gccggcgcct
     9421 tcaaccccgg ccacctcgag gccctgctcg gcaagcactc gctgatcctc atcgacggcg
     9481 cccggcacca gagcgagcgc aagctgctgt cgccgccgct gcacggcgcc cgcatgcggt
     9541 cctacgccgg catcgtccgc gagagtgcat tgcacagggc cgccgcctgg cgggtcggcg
     9601 cgccgctcgt catgcaggac gcgacccagg cgctgtcgct cgatgtcatc gtcaaggcgg
     9661 tgttcggcgt gcaggagccg gcgcgcgtcg ccgccttcga ggccgcgctg ctggcgacga
     9721 tgtcctcggt gtggatgccg gcggtgttga tccgcggcct gcagcgggac ctcgggccct
     9781 tgaccccgtg gcgtcgcttc gcccgcgccc gcgaccacgc cgacgcgctg atccgcgagg
     9841 agatcgccgt ccgccggcgc gcgccgcccg gcgacgacat cctcagcctg atgatctccg
     9901 cccgctacga cgatggcgag ggcatgcgcg acgaccagat ccgcgacgag ctgatgaccc
     9961 tgctcgccgc gggccacgag accaccgcga tcgcgctcgc ctgggcgatg tactggttgc
    10021 atcgccaccc cgacaccctg gagcggctgc gcgccgagct ggccctcgcc ggcgacgacc
    10081 tcgacgcgat cgcctcgctg ccctacctcg acgccgtctg ccacgagacc ctgcgcctgt
    10141 acccgatcgt gccgctcgcc ccgcgcacgc tccggcgccc gctgaccctc ggcccgcaca
    10201 ccctgccgcc cggcacccac gtcggcgcat gcatcgcgct gctgcaccgg caccccgacc
    10261 tctaccccga gcccgatgaa tttcggcccg cgcgctggct cgctcgcaag ttcacgccgt
    10321 gggaattcgc ggtgttcggc ggcggcatcc gccgctgcat cggcgccgcc ttcgcgctgt
    10381 acgcgatgaa gcacgccctc gccgccctgc tcccgcgcca ccgcttccgt ctgctcgagg
    10441 atcgcccggt cacgccggtg cgccgcaaca tcaccctcgg tccgcgcggc gggatccgca
    10501 tggtcctgac cgaacggaca ggtcagcccc ctgcgcccgc cggtgcgcac gtgtcctgag
    10561 acacgtacgc ccggcgggcc gccccgcgcg cagcgttgct ccgtcgccgg gcttccgcta
    10621 gcttgcgcct gcccatgccc gacagcgcgt ccctcgtccg caccgacgcc tcacccgatg
    10681 cgctcgagct gcgccccgtc gacggcttcc cacgctggct cggcacccgc ctcgcgcgct
    10741 tcttcctgcg cgtcgtcagc atggaagaga acgacgccgc gaagctcgcg cgcatggtca
    10801 ccccgacctc gggctacttc cgccgcgacc gccacgtcca ccccgcggtc gacccgacga
    10861 cctacagcgt caagttcacc ggcgtcgccg ctccgcggag cttctcgctc gccgacctcc
    10921 aggccctgcc gcgagagacc cgcgtgtgcg tgcaggagtg cgccggcaac ggcaatcaca
    10981 tcatgggttc ggcgggtctg tgcggacagg ccgtgtggtc gggcccgacc ttcgagagcc
    11041 tgctcgagct gtgcggtggt ccgggcgacg cgacgcactt cgccttccat ggcctcgacg
    11101 acctcggcct ggtcaagcgc ggctatcact acggcctctc gctcgatgaa ctccgccgcg
    11161 cgcaggcgat cgtcgcgctg acgatgaacg gcgagccgct gacgcgccgc catggcttcc
    11221 cggcgcgcct gatcgtcccg cggatctact cgatgtcgca cgtcaagtgg ctcggccacg
    11281 tcgagggcaa gacccgcccg cacatgggga tccacaaccg cttcgtgttc accaacaagg
    11341 agcggcggga cggcaagtgg gtgcgcgtgc aggcgcggtg gatcgggctc aagtcggtga
    11401 tcgcccactg cacgcgcgac ggcagcggct ggcggctgac cggctgcgca tggggtggcg
    11461 agcgaccgat cgccggcgtc gaggtcagca ctgacggcgg cctgtcgtgg cagcccgccc
    11521 gctggcagac cccgatcgaa tattttaatg aacaccccga gctggcgacg cccgagcctg
    11581 aggcgctcgc gggcgcatgg ggcgtgttca cggccacctg gctgccctcc ggccctggca
    11641 cctacaaggt cggctgccgc gcgatcgacg tcgacggcaa cattcagcag ctcgacaacg
    11701 accccaacgt gcacggccac ttcaaccaga cccgggtgaa gtggcgcatg gtcacggtgc
    11761 ccgagcgtcg gctcgggccc gcgggctcct gacgcgcgtc agccgatcag agcacgatcg
    11821 cgcaggcgcc gatgtcttcc tggcccggag ggggcgtgtc ctcgccgtac cacggcacgc
    11881 actcctgtcc cggcatcgcg tccttgccgg ggcaggtgtt cgggtcattc acgttgcagt
    11941 acggggagca gcagccggtc gccatcgtct cggcgcagcc cgggacgagc gtcacgtcgg
    12001 cgcagaacag gccgtagtcg cacacgttga tgtactcgca ggggtcgccg tacttgccct
    12061 gctcgccgct cgcgtcgaag tcgcagataa aggcgtcgac gccgtcgaag aagcagatct
    12121 ggccgtcggg acaggtctgc gccagcgggt cgcaggtgtc gaggcagatg atgatcacgc
    12181 cgtcgttcga ctcgtcgcag gtcttgccgc cggggcacat cggcgcgtcc gcggagccgg
    12241 tgcacatctc gatgcaggtg cccttgttct cggcgtcgag gaaccagcac atcgtcccga
    12301 gcgcgcagtt gtcgacgccc gaggtgccgt cgccctcggc ctgacactcg tcgcccggct
    12361 ggccggggct cgcgtccagc ggcgtacact tggtcgcgtt ccacgagccg ccgccgtcgt
    12421 tcgcgtacgg catgcacttc tcggtcgccg gac
//
LOCUS       DASWNJ010001235         1747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_514810, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001235 DASWNJ010000000
VERSION     DASWNJ010001235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514810"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(892..>1747)
                     /locus_tag="VGB85_20825"
     CDS             complement(892..>1747)
                     /locus_tag="VGB85_20825"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0136545.1"
                     /translation="GAGPFPAVLLLPGSGPIDRNSDHKRMPLGITGQLAQALDAAGLA
                     TFRYDKRGVGASTGRWHCAGFYDNRDDAAAALELLRTHPEVDSDRVLVLGHSEGALHA
                     TGLAAAGAGTAGLVLLSCSAIPGEALLRWQSAQIAPTLPTPVRVLLRLLRTDLEARTA
                     KTHAKIKATTTDEARIEGAKINARWHREFMAHDPRTDLARIRVPVLAVTGGKDLQVPP
                     KDLATIQDLVEAPVETHEMPDLSHILRAQPGPASLSGYKREMRQPVDAELVSLVVGWV
                     SMRVRATA"
BASE COUNT          259 a          542 c          651 g          295 t
ORIGIN      
        1 ccggcgtacc cccggaatgc aacgggggtg gtttcaggca ttgcatgagt tgcgtcgcag
       61 gcgcgcgaga tgcatgagca gatagtggtc tctcgtgatg agaaggtcgt cggttcgatt
      121 ctgacaggcg gctcagccgg aaacagggtc tgaccagcgg aaacgcggtc aggcccttct
      181 tcatgtcaac caattcctgt gccccgggcg tgccccgtgg ggtacgccgt gggtacacga
      241 gagtttcggg cagggctcct tatgtcgttg catgcattgg gtggggcgtc acgcggcgtc
      301 tgtggttgca ggcgttacgg gtcgcctgaa tcatttcggt gagaaggtca gggatgggat
      361 cgcctcgcgc tccatgagtc gtcgaactat cgagagctgc agaacgccgg aagccgcgtc
      421 agttcgggtt gagccatcag tggctcgcac ccggcgcaga gcggctcacc cccgcgtacc
      481 tcggtcccac ctggttcttc aacgcctgac tgcggcccgc gtggtgtgcg ggtggcagct
      541 ggactcgagc gacgggcctc gtatctggcc ggattcgtcg atggctccga cgccaggcgc
      601 tcagcgctgc gccgaacaac aggttaggtg cgcgtctccg cggtgacgct gagttcggcg
      661 gcgcgttccc aggccgcgga cggggcttcg tgcccgccgc ggacccaaga ccgttcggtg
      721 acccggtcga gtgcggaacg ctgtgcaaca cgagtcggtg ccgatcgcgc cccaagcccg
      781 agcccacatc tgcgatgacg gccgatcttg caggcggcgc ggatcggggc aaaccccggc
      841 ggagccgacc gagcgaacga ccgtagcagg gtcgggcgag gaggcttcgc atcaggcggt
      901 agcgcgcacg cgcatgctga cccacccgac gacgagcgac acaagctcag cgtccaccgg
      961 ttggcgcatc tctcgtttgt acccagagag cgacgcaggg ccgggttgcg ctcggaggat
     1021 gtggctcagg tcgggcatct cgtgggtctc gacgggcgcc tccacgaggt cctggatcgt
     1081 agcgaggtcc ttcgggggca cttgaaggtc cttgcccccg gtgacggcga ggacgggaac
     1141 gcggatgcgc gcgaggtcgg ttcgcgggtc gtgggccatg aactcgcggt gccagcgggc
     1201 gttgatcttc gcgccctcga tgcgcgcctc gtcggtcgtt gtggccttga tcttggcgtg
     1261 cgtcttggct gtccgggcct cgaggtcggt tcgcagcagg cggagcagga cgcggacggg
     1321 ggttggcagc gtgggggcga tctgcgcgga ctgccagcgc aggagggctt cgcccggaat
     1381 ggcggagcag ctcaggagga ccaagccagc cgtgcctgcc ccggccgcgg cgaggccggt
     1441 tgcgtgcagg gcgccttctg agtgtccgag gacgagcacg cggtcggaat cgacctcagg
     1501 gtgtgtccgg aggagctcga gcgcggctgc ggcatcgtct cggttgtcgt agaacccggc
     1561 gcagtgccag cggccggtgc tagcccccac gccgcgcttg tcgtaacgga aggtcgcgag
     1621 ccccgccgcg tccagagcct gggcgagctg ccccgtgatg cccaacggca tgcgcttgtg
     1681 gtcggagttt cgatcgatcg gccctgaccc gggcaggagc agcaccgcgg ggaacgggcc
     1741 cgcgccc
//
LOCUS       DASWNJ010001236         7271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_92286, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001236 DASWNJ010000000
VERSION     DASWNJ010001236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7271
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92286"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1871
                     /locus_tag="VGB85_20830"
     CDS             <1..1871
                     /locus_tag="VGB85_20830"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0136546.1"
                     /translation="LDTLGQAEDLALKTDNHWLRFEVLRQQAHALRAAGHGESAEREA
                     RLALALAEQQAWPLRAMALRREFELRSEQAARGDVSALKLRRHLDALLALSLASARAR
                     GFDDQARAAIEELTRILPAERTALFLGGEGEPLVMVAGRDAQGRELPADLEVDRSVLE
                     QVRDSQAPVLARGDRDRRSVLAAPLLLKEALIGIVYLDNRTIRGAFTGDDVEILQAVA
                     GHLAIALETARTTQLEAQIVASVHEKSALIEHATSAVGIGIAVLQADGRLAQVSPTLR
                     EMTRAWPSAEAWWQDAARQLLLPEPTPCPRCGEQQNLGRSVADLRHGGERQVFEVTFT
                     GHFHEFTRAEAGHVLLVSDITRRKISEENLLRLNEELTHTRDEALAASRAKSTFLANM
                     SHELRTPLNAIIGFAEMLVEDAEHLGARHMVADLGKIRMSGTHLLELISTILDLSKVE
                     TGRMELEILEFSLVGLVNRVAAMLTPMVAKNRNMLSHTVAKGVDLMTGDETKVKQILF
                     NLLSNAAKFTRDGVIKLDAEPLHEDGRDWVICTVSDTGIGFRADQIDKLFQDFYQADM
                     STTRKYGGTGLGLAIVHRFCGLMEGEVTVTSEPGIGSSFRVKLPRVLSKPGRPAPP"
     gene            1936..2658
                     /locus_tag="VGB85_20835"
     CDS             1936..2658
                     /locus_tag="VGB85_20835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136547.1"
                     /translation="MAFRKFCALGLAATVAVMVAVACDPTEEPETGECALAEGVVAHF
                     SIDAGGWPASGDNLEIAAACTVGAVGPTISLDCDDGGATRAVHVTLMAAPALAVPVEV
                     GASVDLVLRRKADAAPDRGFFRLRGSDGALLLAGARSYSSTPGADAQFFAPLKVSLDL
                     KTCEERKTDECRLEQKVVLVVDDGAASTRVPHGSATTLPSGYELRVGRAMLSQASGDP
                     KVCPLDDTTPETYEYLIAAPAK"
     gene            complement(2662..3465)
                     /locus_tag="VGB85_20840"
     CDS             complement(2662..3465)
                     /locus_tag="VGB85_20840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136548.1"
                     /translation="MLLTALSLLHCLGLAYAAEDPGRVDPDRPNIANSTKTVAPGAVQ
                     IEAGVDAQVLGKARMDSFRFSAPLTLRIGVHEKIELRIADGDPWRWAQGLLGARQQGE
                     ISLGAKLRLYEKETGTKVSLGVLPQLIPTSPRGEARFWAPLPGAVFLTTVEPGDWHLD
                     FNAGIKMRANEKGKCCDAEGVLAASFGRGFADEVVLLWIEAYSRTNLGQGELAEIAGD
                     AGIIVTPTRRVALDLAVLVSQVNKTLVTALLAGITVRMGPMGPWWSRRR"
     gene            complement(3471..3821)
                     /locus_tag="VGB85_20845"
     CDS             complement(3471..3821)
                     /locus_tag="VGB85_20845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136549.1"
                     /translation="MRITPQKNHLAAGVALLLTATVPSCGDDTEPGTESATDGGGTGT
                     SATATATAGTATTDSGTASGTAGTATGGTATGGTATGGSGGSGGSGGSGGSGAQSDEA
                     PPPDEPSRPVTAPR"
     gene            4119..6521
                     /locus_tag="VGB85_20850"
     CDS             4119..6521
                     /locus_tag="VGB85_20850"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0136550.1"
                     /translation="MPWRWSTPAGTSSTPTRRWRCSRVTRAPRSTGRAFGRCFRAGST
                     TRSGPRCARRSTATPRRRVARTGSRTAAGSSSTRCPRTAACSSPCATSASARDPRRPA
                     ATPTSAAASPSPARAWAPGSGRSPPTRCAGRRSSGACTAARRAPSTGRSTSSSTASTR
                     RGATACSGLRASIDEGRELHMEYLLRRPDGSTTWIETHGRVLRDEAGQPQRMIGVSAD
                     INERKRIEEALRNSEEAHRFLTESVPTQVWTARPDGQLDHVNRQVLEYFDKTSEAMLG
                     EGWQHVIHPADLAECVKRWTRSLTTGEPYEVEFRLRRSDGEYRWHIARARPLRDRDGQ
                     IVRWFGTNTDIDEQKRVGQAQRFIIEASNVLAASLDYETTLASVARLAVPEIADWCAV
                     DLIDPEDPTRLRRVTVAHVDPAKVALAHEMHRRYPIDMNAQSGVPQVIRSGKPEVFEV
                     IEDWMLVQSCRDPEELRLARELGLTSSMAVPLRARGRTLGAISLVAAEPGRHFDQEDL
                     RVAEALALRCAVAIDNAILLRQAQEAEQDLRRLNEDLERRVAERTADVKKAKERVEEA
                     NARLKELDTMKDEFLGALSYQLASPINAVIGYTDLLLEGAGGQLREEQRRYVRRITAS
                     SKVLLSLVHDLLDMSRISAGKFELDRESLDPGALAREVLDFLEPLTEMQGLRVVSRIA
                     DDLRGIPGDAQRLEQVLANVMHNAIQLTSSGGLLRLSVERAGDVIRFEVHHTGTTLAP
                     DDLEKIFRRFTRLGGAWLGLSIARRIIETHGGAMGVQPHAGGGGGNTFWFTIPAPVRV
                     KA"
     gene            6573..>7271
                     /locus_tag="VGB85_20855"
     CDS             6573..>7271
                     /locus_tag="VGB85_20855"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0136551.1"
                     /translation="MIHGTQMKLAGRVVAATVAGAIGLAAGAVRLRRRARRIDLAGRV
                     VVVTGGGRGFGYAIAREFLRHGCKLAIGSRDGETIARATADFRLHGADVFGMACDASD
                     PLQVRDFIAAVLERFGGIDILVNNAGQVFFGPAAELHPDDLESAMRNIFWTHYRPTMA
                     VLPYMRARGFGRIVNITSLAGKVPLPHHGAYVAGKHAATGWSETLAIELRKDGILVST
                     ITPPALNDGAPLHTH"
BASE COUNT         1043 a         2601 c         2610 g         1017 t
ORIGIN      
        1 cgctggacac cctggggcag gcggaagatc tcgcgctcaa gaccgacaat cactggctgc
       61 gcttcgaggt gttgcggcag caggcgcacg ctttgcgggc ggccgggcac ggcgagtcgg
      121 ccgagcgcga ggcccggctg gcgctggcgc tggccgagca gcaggcctgg ccgctgcggg
      181 cgatggccct gcgccgggag ttcgagctgc gctccgagca ggcggctcgc ggcgacgtct
      241 cggcgctcaa gctgcggcgc cacctcgatg cattgttggc cctgagcctc gcctcggccc
      301 gtgcgcgggg cttcgacgac caggcgcggg ccgcgatcga ggagctgacg cggatcctgc
      361 ccgcggagcg cacggcgctg ttcctcggcg gcgagggcga gccgctggtg atggtcgccg
      421 gccgcgacgc ccaggggcgc gagctgccgg ccgacctcga ggtcgaccgt tcggtgctcg
      481 agcaagtacg tgacagccag gcgccggtgc tcgcgcgtgg ggaccgtgat cggcgcagcg
      541 tcctcgcggc accgctcttg ctcaaggagg cgctgatcgg catcgtgtac ctcgacaacc
      601 gaaccattcg cggcgccttc accggcgacg acgtggagat ccttcaggcc gtcgccggcc
      661 acctcgccat cgccttggag accgcccgca ccacgcagct agaagcgcag atcgtcgcct
      721 cggtgcacga gaagtcggcg ttgatcgagc acgccaccag cgcggtcggc atcggcatcg
      781 ccgtgctgca ggccgacggt cggctcgcgc aggtcagccc gacgctgcgc gagatgacgc
      841 gcgcgtggcc gtcggcggag gcgtggtggc aggacgcggc gcgccagctg ctgctgcccg
      901 agccgacgcc gtgcccgcgc tgcggcgagc agcagaacct cgggcgcagc gtcgccgacc
      961 tgcgccacgg cggcgagcgt caggtcttcg aggtcacctt caccggtcac ttccacgagt
     1021 tcacgcgggc cgaggccggc cacgtgctgc tggtctcgga catcacgcga cgaaagatca
     1081 gcgaggagaa cttgctccgg ctaaatgagg agctgaccca cacgcgcgac gaggcgctgg
     1141 cggccagccg ggccaagagc accttcctcg ccaacatgtc gcacgagctg cgcacgccgc
     1201 tcaacgcgat catcggcttc gcggagatgc tggtcgagga cgcggagcac ctcggcgcgc
     1261 gccacatggt cgccgacctc ggcaagatcc ggatgtcggg cacgcacctg ctcgagctga
     1321 tcagcacgat cctcgacctc tccaaggtcg agaccgggcg catggagctc gagatcctcg
     1381 agttctcgct cgtcggcctc gtcaaccgcg tcgccgcgat gctgacgccg atggtcgcca
     1441 agaaccgcaa catgctgtcg cacacggtcg ccaaaggcgt cgacctgatg accggcgacg
     1501 agaccaaggt caagcagatc ctgttcaatc tgctgtcgaa cgcggccaag ttcacccgcg
     1561 acggcgtcat caagctcgac gcggagccgc tgcacgagga cggccgcgac tgggtgatct
     1621 gcacggtcag cgacaccggc atcggctttc gtgccgacca gatcgacaag ctgttccagg
     1681 acttttatca ggccgacatg tcgacgaccc gcaagtacgg cggcaccggc ctcggtctgg
     1741 cgatcgtgca ccgcttctgc gggctgatgg aaggcgaggt caccgtgacc agcgagccgg
     1801 gcatcggctc cagcttccgg gtcaagctgc cgcgcgtgct cagcaagccc ggccgcccgg
     1861 ccccacccta gaggcgtgat ctcgcccgat gtggccggcc gtgacgaccg cggggtctgg
     1921 tcgtatgctc cggccatggc tttcaggaag ttctgtgcgc tcgggctcgc ggccactgtc
     1981 gcggtcatgg tcgcggtcgc gtgtgacccg accgaggagc cggagaccgg cgagtgcgcg
     2041 ctggcggagg gcgtcgtggc ccacttctcg atcgacgctg gcgggtggcc ggcgagcggc
     2101 gacaacctcg agatcgcggc ggcctgcacc gtcggcgcgg tcggcccgac gatctccctc
     2161 gactgcgacg acggcggcgc cacgcgggcc gtccacgtga cgttgatggc cgcgcccgcg
     2221 ctcgcggtgc cggtggaggt cggcgcgagc gtcgacctcg tgctgcggcg caaggccgac
     2281 gccgcccccg accgcggctt ctttcgcctg cgcggcagcg acggcgccct gctgctggcg
     2341 ggcgcccgca gctactcgag cacgcccggc gccgacgcgc agttcttcgc gccgctcaag
     2401 gtctccctcg acctcaagac ctgcgaggag cgcaagaccg acgagtgccg gctcgagcaa
     2461 aaggtcgtgc tggtggtcga cgacggcgcc gcgagcacgc gcgtgccgca cggcagcgcg
     2521 acgacgctgc cctcggggta cgagctgcgg gtcgggcggg cgatgctcag ccaggcgagc
     2581 ggggacccga aggtgtgccc gctcgacgac acgacgcccg agacctacga gtacctgatc
     2641 gccgcgccgg cgaagtgagc gtcagcgccg gcgagaccac cacggtccca tcggtcccat
     2701 gcgcaccgtg atgccggcga gcagcgcggt gacgagggtc ttgttgacct ggctgacgag
     2761 gacggcgagg tcgagggcga cgcgccgcgt cggtgtcacg atgatgcccg cgtcgcctgc
     2821 gatctcggcg agctcgccct gtccgaggtt ggtgcggctg taggcttcga tccacagcag
     2881 cacgacctcg tcggcgaagc cgcggccgaa gctggccgcg agcacgccct cggcgtcgca
     2941 gcacttgccc ttctcgttgg cgcgcatctt gatgccggcg ttgaagtcga ggtgccagtc
     3001 gcccggctcg acggtggtca ggaacacggc cccgggcaag ggcgcccaga acctggcctc
     3061 cccgcgcggc gaggtcggga tcagctgcgg caggaccccg agcgagacct tcgtgccggt
     3121 ctccttttcg tacagacgaa gcttggcgcc gagcgagatc tcaccctgct ggcgcgcgcc
     3181 gagcaggccc tgggcccagc gccacggatc accgtcggcg atccgcagct cgatcttctc
     3241 gtgcacgccg atcctcaggg tcagcggcgc cgagaatcgg aacgagtcca tcctggcctt
     3301 gccgagcacc tgcgcgtcca cgccggcctc gatctgcacc gcgccggggg cgacggtctt
     3361 ggtcgagttg gcgatgttcg gccggtcggg gtcgacgcgg ccgggatcct cggccgcata
     3421 tgccagtccg aggcagtgca ggaggctgag tgcggtcagg agcatccggg tcagcggggc
     3481 gccgtgaccg gacggctcgg ctcgtccggg ggaggagcct cgtccgactg cgcgccgctt
     3541 ccaccactac cgccactacc accacttccg ccacttccgc cggtcgcggt gccgccggtc
     3601 gcggtgccgc cggtcgcggt gcctgcggtg ccgctcgcgg tgccgctgtc agtagtcgcg
     3661 gtgcctgccg tggcagtcgc ggtcgcggag gtaccggtgc cgccgccgtc ggtggcgctc
     3721 tcggtgccgg gttcggtgtc gtcgccgcag ctggggacgg tggccgtcag caacaaggcg
     3781 acgccggccg cgaggtgatt tttttgagga gtgatgcgca tacgagttcc tttgttgccc
     3841 ggctcatgga tgaggaccgc cgcggcgcaa cgccgacggg gcgggcggcg aggtcgggac
     3901 gccggggcgc gaacgcgccg gccgtgaacg cttcaccggc gcggacagcg aacgtttcgc
     3961 gggctttcgc gctatgggcg cggcttcggt aggaatcgcc gatactggcg gttttactgg
     4021 ccggttcgct cgcaaggacc gcccggggtt ataaagcccg ggcttgtccg cccccgagac
     4081 cgaggcccga ctcaccgctt gcctggaccg ggcgaccgat gccctggcgc tggtcgacgc
     4141 cggctggcac atcctctacg ccaacccggc gctggcgctg ctcgcgggtc acccgggcgc
     4201 cccgctccac ggggagagct tttggccgct gtttccgggc tgggtcgacc acccgctcgg
     4261 gaccgcgctg cgcgaggcgg tcgacggcca caccgcgacg cagggtggcc cgcaccgggt
     4321 cccggacggc cgctgggtcg agttcaacgc ggtgccctcg gactgcggcg tgctcgtcac
     4381 cctgcgcgac gtcagcgagc gccagagatc cgaggaggcc cgccgcgacg ccgacgagcg
     4441 cagccgcttc gccctcgcca gcacgcgcat gggcacctgg gagtggacgc tcgccaccaa
     4501 ccaggtgcgc tggtcggagg agctcgggcg cctgtacggc cgctcgccgg gcgccttcga
     4561 cgggccgttc gacttcgtcg tcgacagcgt cgacccggcg tggcgcgacc gcgtgctcgg
     4621 gcctgcgcgc gagcatcgac gagggccgcg agctgcacat ggagtacctg ctgcgccgcc
     4681 cggacggcag caccacctgg atcgagaccc acggccgtgt gctgcgcgac gaggccggcc
     4741 agccgcagcg catgatcggc gtgtccgccg acatcaacga gcgcaagcgg atcgaggagg
     4801 ccctgcgcaa cagcgaggag gcccaccgct tcctcaccga gtcggtcccg acccaggtgt
     4861 ggaccgcccg ccccgacggc cagctcgacc acgtcaaccg ccaggtcctc gagtacttcg
     4921 acaagaccag cgaggcgatg ctcggcgagg gctggcagca cgtcatccac ccggccgacc
     4981 tcgcggagtg cgtcaagcgc tggacccgct cgctgacgac cggcgagccc tacgaggtcg
     5041 agtttcgcct gcgccgctcc gatggcgagt accgctggca catcgcccgc gcccgcccgc
     5101 tgcgcgaccg cgacggtcag atcgtccggt ggttcggcac caacacggac atcgacgagc
     5161 agaagcgcgt cggccaggcc cagcgcttca tcatcgaggc gagcaacgtg ctcgcggcct
     5221 cgctcgacta cgagacgacg ctcgcgagcg tcgcccgcct cgcggtgccg gagatcgccg
     5281 actggtgcgc ggtcgacctg atcgacccgg aggacccgac gcgcctgcgc cgggtcaccg
     5341 tcgcccacgt cgacccggcc aaggtcgcgc tcgcccacga gatgcaccgc cgctacccga
     5401 tcgacatgaa cgcccagagc ggcgtgccgc aggtgatccg cagcggcaag cccgaggtct
     5461 tcgaggtgat cgaggactgg atgctcgtgc agagctgccg cgaccccgag gagctgcgcc
     5521 tcgcgcgtga gctcggcctg acctcgtcga tggcggtgcc gctgcgggcc cgcggccgca
     5581 cgctcggcgc gatctccctc gtcgccgccg agccggggcg gcacttcgat caggaggacc
     5641 tgcgcgtcgc cgaggcgctg gcgctgcgct gcgccgtggc gatcgacaac gcgatcctgc
     5701 tgcgccaggc ccaggaggcc gagcaggacc tgcgccgcct caacgaggac ctcgagcgcc
     5761 gcgtcgccga gcgcaccgcg gacgtcaaga aggccaagga gcgggtcgag gaggccaacg
     5821 cgcggctcaa ggagctcgac acgatgaagg acgagttcct cggcgcgctg tcgtaccagc
     5881 tcgccagccc gatcaacgcg gtgatcggct acacggacct gctgctcgag ggcgccggcg
     5941 gtcagctgcg cgaggagcag cgccgctacg tccgccggat cacggcgtcg tcgaaggtgc
     6001 tgctgtcgct ggtgcacgac ctgctcgaca tgagccggat ctcggccggc aagttcgagc
     6061 tcgaccgcga gagcctcgac cccggcgcgc tcgcccgcga ggtgctcgac ttcctcgagc
     6121 cgctgaccga gatgcagggc ctgcgcgtgg tctcgcggat cgccgacgac ctgcgcggga
     6181 tcccggggga tgcccagcgg ctcgagcagg tgctcgccaa cgtgatgcac aacgcgatcc
     6241 agctgacctc ctcggggggg ttgctgcgcc tgtcggtcga gcgcgcgggg gacgtgatcc
     6301 gcttcgaggt ccaccacacc ggcacgacgc tcgcgccgga cgacctcgag aagatcttcc
     6361 gccgcttcac ccgcctcggc ggcgcgtggc tcggcctgtc gatcgcgcgc cgcatcatcg
     6421 agacccacgg cggggcgatg ggcgtccagc cccatgccgg cggcggcggc ggcaacacgt
     6481 tctggttcac gatcccggcg cctgtgcgcg tgaaggcgtg agccggggcg aggcggtttg
     6541 cctcgcccgg tttctcggcc acgaacatgg gcatgatcca cgggacgcag atgaagctgg
     6601 cagggcgcgt cgtcgcggcg acggtggcgg gcgcgatcgg cctcgcggcc ggggcggtgc
     6661 ggctgcgccg gcgagcgcga cgcatcgacc tcgccgggcg ggtggtcgtc gtcaccggag
     6721 gcgggcgcgg cttcggctac gcgatcgcgc gcgagttcct gcgccacggc tgcaagctgg
     6781 cgatcggcag ccgcgacggc gagacgatcg cgcgggccac cgccgacttt cgcctgcacg
     6841 gcgccgacgt gttcggcatg gcctgtgacg ccagcgaccc cctgcaggtc cgcgacttca
     6901 tcgccgccgt gctcgagcgc ttcggcggca tcgacatcct cgtcaacaac gccggtcagg
     6961 tgttcttcgg gccggccgcc gagctgcatc cggacgacct cgagtccgcg atgcgcaaca
     7021 tcttctggac gcactaccgg ccgacgatgg cggtcttgcc gtacatgcgc gcgaggggct
     7081 tcgggcgcat cgtcaacatc acctcgctcg ccggcaaggt gccgctgccg caccacgggg
     7141 cgtacgtcgc cggcaagcac gcggcgaccg gctggtcgga gaccctggcg atcgagctgc
     7201 gcaaggacgg catcctcgtc agcacgatca ccccgccggc gctcaacgac ggcgcgccgc
     7261 tgcacaccca c
//
LOCUS       DASWNJ010001237         2233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_162057, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001237 DASWNJ010000000
VERSION     DASWNJ010001237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162057"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2233)
                     /locus_tag="VGB85_20860"
     CDS             complement(<1..>2233)
                     /locus_tag="VGB85_20860"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY0136552.1"
                     /translation="VPAWIGPLLLRGLAKDPARRFPSLAELVVELARDPEAERARRRR
                     LALQLLATAAMTVLMIVGGIRLYGVLVERAAERRADARLEQLHEQWASARAQGDTAKA
                     DRLLAAFVEFPDNRGTAAVSRAYREYAAELTDPAAAVDAFAGAYLTATDRDDEISALR
                     GLIPRLVAAGRHLEAGAALATLDRRAPELAADPELTLARSAAALRSRDLPTAAAALAM
                     LAPDDPTRAYGQVLENLFRATAITVEQFEGGPVRMGLDAADLDGDGRSEVATLAAQGP
                     GFFALTTGAGGLSSLGVIRGGPGDPKISLLPHTLAGAPMAILVGPGDGLAIDRSHRLV
                     ELLADGGTVTRLAWPDSKLRAPVVTDLDGDGTPEIYIGSGAYARRLSRIDRGPGGSWR
                     RRSVHPEIDRIASDINPLVAGDLDGDGRDELVVALGAWRAFDVRVLRGGPDGAIDQVA
                     RKTTGFVSALGLVRVGDETWIAALKRDENASAARFSPTAPYGPPAGIYLFALRDDALV
                     EVGFVPSRPPELESFLQPLLVTDLDGDGHDDLVATALASGDVEVHLAVLRQHAGGFLP
                     PLLIRGLEPQLLVEVDGDPARELIVGGERLRPTEHFVLGLGDAPLPPLPVTRSEPRPL
                     PAAVDDPTLAAAWRHAEEMVAVGLTHRSAVELAGLAGLAGHVREDLLLRAGELEALAG
                     DHKGAAERFIAAAERPDLADQALAGAIRSRRALGEVDAEAALVARRAALPGLSPDDRR
                     AAQA"
BASE COUNT          280 a          814 c          885 g          254 t
ORIGIN      
        1 ggcctgcgcc gcgcggcggt cgtcgggtga cagcccgggc agcgccgcac gacgggcgac
       61 gagcgccgcc tcggcgtcga cctccccgag cgcgcgccgg ctgcggatcg cccccgcgag
      121 cgcctgatcg gcgaggtccg ggcgctcggc cgccgcgatg aagcgctccg ccgcgccctt
      181 gtggtcgccc gcgagcgcct ccagctcacc tgcccgaagg agcaggtcct cgcggacatg
      241 tcctgcgaga ccggcgagcc cggccagctc gaccgcgctg cggtgcgtca ggccgaccgc
      301 gaccatctcc tcggcgtggc gccacgccgc cgcgagggtc gggtcgtcga cggccgccgg
      361 cagcgggcgc ggctcgctgc gcgtcaccgg cagcggcggc agcggggcgt cgccgaggcc
      421 gagcacgaag tgttccgtcg gtcgcaggcg ctccccgccg acgatcagct cgcgggcggg
      481 atcgccatcg acctcgacga gcagctgcgg ctcgaggccg cggatcagca gcggcggcag
      541 gaagccgccg gcgtgctggc gcagcacggc gaggtggacc tcgacgtcgc cggacgcgag
      601 cgcggtcgcc acgaggtcat cgtgaccgtc gccgtcgagg tcggtgacca gcaggggctg
      661 gaggaagctc tcgagctcgg gcgggcggct cggcacgaag ccgacctcga cgagcgcgtc
      721 gtcacgcagc gcgaacaggt agatgccggc gggcgggccg tacggtgcgg tcggcgagaa
      781 gcgggccgcg ctggcgttct cgtcgcgctt gagcgccgcg atccacgtct cgtcgccgac
      841 gcgcaccagc ccgagcgcgg acacgaagcc ggtggtcttg cgggcgacct gatcgatcgc
      901 gccgtcgggt ccgccgcgga gcacccgcac gtcgaaggcc cgccacgcgc cgagcgcgac
      961 gaccagctcg tcgcgtccgt caccgtcgag gtcgccggcg accagcgggt tgatgtcgga
     1021 cgcgatccgg tcgatctccg gatgcaccga ccgacggcgc cagctcccgc cggggccgcg
     1081 atcgatccgc gacagccggc gggcgtacgc gccggagccg atgtagatct ccggcgtacc
     1141 gtcgccgtcg aggtcggtga cgacgggggc ccgcagcttc gagtcgggcc aggccaggcg
     1201 cgtgacggtc ccgccgtcgg cgagcagctc gacgaggcga tgcgagcggt cgatcgccag
     1261 gccgtcgccg ggccccacga ggatcgccat cggcgcgccg gcgagcgtgt gcggcagcaa
     1321 gctgatcttg gggtcaccgg gcccgccgcg gatcacaccg agcgacgaca gccccccggc
     1381 gccggtcgtc agcgcgaaga agcccgggcc ctgggccgcc agggtcgcca cctcgctgcg
     1441 gccatcgccg tcgaggtcgg ccgcgtcgag gcccattcgc accggaccgc cctcgaactg
     1501 ctccacggtg atcgctgtcg ctcggaacag gttctcgagg acctgtccat aagcgcgggt
     1561 cgggtcgtcc ggggcgagca tcgctagcgc ggcggcggcg gtcggcaggt cgcggctgcg
     1621 cagcgcggcg gcgctgcggg cgagggtcag ctcggggtcg gcggcgagct cgggcgcgcg
     1681 gcggtcgagg gtcgccaggg ccgcgccggc ctcgaggtgg cgcccggcgg cgacgagccg
     1741 cgggatgagc ccgcgcagcg cagagatctc gtcgtcgcgg tcggtcgcgg tcaggtaggc
     1801 gccggcgaac gcgtcgacgg ccgcggcggg gtcggtgagc tcggcggcgt actcacggta
     1861 ggcgcgcgac acggccgcgg tgccgcggtt gtcggggaac tcgacgaagg ccgcgagcag
     1921 gcggtcggcc ttcgcggtgt cgccttgcgc gcgcgcggag gcccattgct cgtgcaattg
     1981 ttcgaggcgc gcgtcggccc ggcgctccgc ggcgcgctcg acgagcaccc catacagccg
     2041 gatcccgccg acgatcatca gcaccgtcat cgccgcagtg gcgagcagtt gcagcgcgag
     2101 ccggcgtctg cgcgcgcgct cggcctcggg gtcgcgcgcg agctcgacga cgagctcggc
     2161 gagcgagggg aagcggcgcg cggggtcctt ggcgagccca cgcagcagca gcgggccgat
     2221 ccacgccggc acc
//
LOCUS       DASWNJ010001238         2293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_199571, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001238 DASWNJ010000000
VERSION     DASWNJ010001238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199571"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VGB85_20865"
     CDS             complement(<1..194)
                     /locus_tag="VGB85_20865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136553.1"
                     /translation="MAPPLLPVTRLVVCAACAEHVKVSDDRCPHCGVTLPGRAGLMAR
                     AAGVALAGVMLVGCSEAMSV"
     gene            complement(249..740)
                     /locus_tag="VGB85_20870"
     CDS             complement(249..740)
                     /locus_tag="VGB85_20870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136554.1"
                     /translation="MAPPLLPNLLPATRLIACSACAQHVKSNESSCPHCGAELRGSES
                     RALRAAGAVLLGLALSGCADKEPDTASDSEGMTGTSGTSGATDATGSDTGATGTGSDS
                     SGDTSTGGPEPEYGVPMTTSEPDYGVPSTDPTTGDTDASTGDTASDTSSGSGEPLYGA
                     GAT"
     gene            873..1310
                     /locus_tag="VGB85_20875"
     CDS             873..1310
                     /locus_tag="VGB85_20875"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEY0136555.1"
                     /translation="MTRDPVFFAYTLTVVVNVLNVMTLWNLSGAARARTRTTLNPEDA
                     ATVVRGAAVVEAEPPAVARVLRAHRNSFDNTIPFLLLGLLFVFQRPNLLEAQILFGAF
                     TAARVAFSITYLAGLQPWRTISYILGVLSTLALIVEVCRHAFA"
     gene            complement(1340..1624)
                     /locus_tag="VGB85_20880"
     CDS             complement(1340..1624)
                     /locus_tag="VGB85_20880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136556.1"
                     /translation="MLHLPTGPNPAVEVDGYSLDEIGERIGRAIARLLRALGEPAEAE
                     PYVRAWIAGLDSGTRSRFIIRVLAPDWPDGSDSLVAAVREGLAVRRSPGH"
     gene            1859..>2293
                     /locus_tag="VGB85_20885"
     CDS             1859..>2293
                     /locus_tag="VGB85_20885"
                     /inference="COORDINATES: protein motif:HMM:NF018271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136557.1"
                     /translation="MLAQFCIYGAFGWCAEIVWTALYELVTGLRSDPCDPDAFVAVPP
                     AERWRLTGHTYLWMFPIYGAGGLMFGPLHDALRDMVWWQRGVLWTVLIFATEYLSGWV
                     LRRVTGRCPWDYSYTRRHLHGLIRFDYTPVWFAFGLVFERLHD"
BASE COUNT          303 a          888 c          743 g          359 t
ORIGIN      
        1 tacaccgaca tcgcctcgct gcacccgacg agcatcaccc ccgcgagcgc gacccctgcc
       61 gcgcgggcca tcagcccggc gcgccccggc agcgtcacgc cgcagtgcgg gcagcggtcg
      121 tcggagacct tgacgtgctc ggcgcacgca gcacacacga cgagacgcgt gacgggtaac
      181 agtggtggtg ccatcgccgt cacgagacca cgcggcccgc ggaccgtcaa gcgcgcgcgc
      241 ccccgccctc aggtcgcgcc ggcgccgtag agcggttcac cacttccgct cgaggtgtcg
      301 ctcgcggtgt cgcccgtgct cgcgtcggtg tcccccgtcg tcggatccgt cgagggcacg
      361 ccgtagtcgg gctcggaggt cgtcatcggc acgccgtact cgggctcggg cccgccggtg
      421 ctcgtgtcgc ccgaactgtc gctcccggtc cccgtcgccc cggtgtcact accggtcgca
      481 tcggtggcgc ccgaggtgcc cgatgtcccg gtcatgccct cgctgtcgct cgcggtgtcg
      541 ggctccttgt ccgcgcagcc cgacagcgcc aggccgagca gcaccgcacc ggcggcgcgc
      601 agcgctcgac tctcgctgcc gcgcagctcc gccccgcaat gtgggcagct cgactcgttc
      661 gacttgacgt gctgtgcaca cgccgagcac gcgatcagac gcgtggccgg caggaggttc
      721 ggaagcagtg gtggcgccat gggatcccgg ccgccagcgt cgcacgcagc cccggcgggg
      781 tcaacgccgg ccctggaccc cacccggacg cccgcaaacc cgggtccgcc gccccctgcc
      841 ctgcgccgcg tatcggccta tgatccgcgg ccatgacccg agatcccgtg ttcttcgcct
      901 acacgctgac cgtcgtcgtc aacgtcctca acgtcatgac gctgtggaac ctcagcggcg
      961 ccgcgcgggc gaggacgcgg accaccctca accccgagga cgccgccacg gtggtccgcg
     1021 gcgccgccgt cgtcgaggcc gagccgcccg cggtcgcgcg cgtgctccgg gcccaccgca
     1081 acagcttcga caacacgatc cccttcctgc tgctcggcct gctgttcgtg ttccagcgcc
     1141 ccaacctgct cgaggcgcag atcctgttcg gcgcgttcac ggccgcccgc gtcgccttct
     1201 cgatcaccta cctcgccggc ctccagccgt ggcggacgat cagctacatc ctcggcgtgc
     1261 tctctaccct cgcgctgatc gtcgaggtct gccgccacgc cttcgcatga gcgagcatgc
     1321 gtaacgtgat tgagccgagt cagtggcccg gcgagcgccg gaccgccagc ccctcgcgca
     1381 cggcggcgac cagcgagtca ctgccgtccg gccagtcggg cgcgagcacc cggatgatga
     1441 agcggctgcg cgtgcccgag tcgagccccg cgatccacgc ccggacatac ggctctgcct
     1501 ctgcgggctc acccagcgcc cgcaacaggc gggcgatcgc ccgaccgatg cgctcgccga
     1561 tctcgtcgag gctataacca tccacttcca cggcggggtt gggtccggtg ggtagatgca
     1621 gcatgacccc tgagtatcct cgtcttcgct cgctgtctgc tgggaatgtc ccgaatgtct
     1681 tgaataaccg gatgcgccga atgcccgtac cacggcgcgt cggccgaccc gcgctgctgc
     1741 cgagtcgccg agaaccccgg tggagcggcg ttacgcggct atcacggcac gtacacgcca
     1801 gcggcgcccg cgatcgcttg cccgtccgtc ggactgcgac atgacggcgt gagaccccat
     1861 gctggcacag ttctgcatct acggagcctt cggctggtgc gccgagatcg tgtggacggc
     1921 cctctacgag ctcgtcaccg gcctgcgcag cgacccctgc gaccccgacg cattcgtggc
     1981 ggtgccgccg gccgagcgct ggcgcctgac cggccacacg tacctgtgga tgttcccgat
     2041 ctacggcgcc ggcggcctga tgttcggccc gctgcacgac gcgctgcgcg acatggtctg
     2101 gtggcagcgc ggggtcctgt ggaccgtcct gatcttcgcc accgagtacc tcagcggctg
     2161 ggtcctgcgc cgcgtgaccg ggcgttgccc gtgggactac agctatacgc gccgccacct
     2221 ccacgggctg atccgcttcg attacacgcc ggtgtggttc gccttcggcc tcgtcttcga
     2281 gcgcctgcac gac
//
LOCUS       DASWNJ010001239         1766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_162100, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001239 DASWNJ010000000
VERSION     DASWNJ010001239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162100"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /gene="ispG"
                     /locus_tag="VGB85_20890"
                     /gene_synonym="gcpE"
     CDS             <1..1005
                     /gene="ispG"
                     /locus_tag="VGB85_20890"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEY0136558.1"
                     /translation="ADVNATLQQIAELTASGCQIVRVAVPDPVDAEALPRVVKQSQIP
                     VIADIHFQSKYIFAAIDAGCAAVRVNPGNIREFDGKVGEVARAAKASGTPIRIGVNAG
                     SLDKRIMAKHGKATPEALVESALWECSLFEEHDFRDIKISVKHNDPVVMVQAYRLLSE
                     RCDYPLHLGVTEAGPAFQGTIKSAVAFGALLAEGIGDTIRVSLSAPPVEEVKVGLAIL
                     ESLNLRQRGLEIVSCPSCGRAQVDVYTLAEQVTAGLEGLTVPLRVAVMGCVVNGPGEA
                     READLGVASGNGKGQIFVKGEVIKTVPESQIVETLIEEAMRLAEDYDGASGEPSVGVS
                     "
     gene            1062..>1766
                     /locus_tag="VGB85_20895"
     CDS             1062..>1766
                     /locus_tag="VGB85_20895"
                     /inference="COORDINATES: protein motif:HMM:NF024708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4081 domain-containing protein"
                     /protein_id="HEY0136559.1"
                     /translation="MLRTTAPVRLLERRDTDEVLDLLDRDPVADVFVASRVRGAGLAE
                     SRLGAQVWGYGERGRLEALCYSGANLVPVQAGPEAVRAFAERARRQGRRCSSIVGPAD
                     AVGELWRLLESSWGPAREVRDCQPLLAIDGPPALPADPRVRPVRPDELDVLLPACVAM
                     FTEEVGVSPTAGDGGALYRARVRELIAQGRAYARIEDGRVLFKAEVGAATATTCQVQG
                     VWVDPALRGRGLGTAGT"
BASE COUNT          228 a          643 c          668 g          227 t
ORIGIN      
        1 gccgacgtca acgccacgct gcagcagatc gccgagctca cggcgtccgg ctgccagatc
       61 gtccgcgtcg cggtgcccga cccggtcgac gccgaggcgc tgccccgcgt cgtgaagcag
      121 tcgcagatcc ccgtgatcgc cgacatccac ttccagagca agtacatctt cgcggccatc
      181 gacgccggct gcgccgccgt gcgcgtcaac cccggcaaca tccgggagtt cgacggcaag
      241 gtgggcgagg tggcccgcgc ggccaaggcc agcggcacgc cgatccgcat cggggtgaac
      301 gccggcagcc tcgacaagcg gatcatggcc aagcacggca aggcgacgcc ggaggcgctc
      361 gtcgagtcgg cgctgtggga gtgctcgctg ttcgaggagc acgacttccg cgacatcaag
      421 atctcggtga agcacaacga cccggtcgtg atggtccagg cctaccggct gctgtccgag
      481 cgctgcgact acccgctcca cctcggcgtc accgaggcgg gcccggcctt ccagggcacg
      541 atcaagtcgg cggtggcctt cggcgccctg ctggccgagg gcatcggcga cacgatccgg
      601 gtctcgctga gcgcgccgcc ggtcgaggag gtcaaggtcg gcctcgcgat cctcgagtcg
      661 ctcaacctgc gccagcgcgg gctcgagatc gtgtcctgcc cgtcctgcgg ccgggcccag
      721 gtcgacgtct acaccctcgc cgagcaggtg accgccgggc tcgaggggct caccgtcccg
      781 ctgcgcgtcg ccgtcatggg ctgcgtcgtg aacggcccgg gcgaggcccg cgaggccgac
      841 ctcggcgtcg cgtccggcaa cggcaagggg cagatcttcg tcaagggcga ggtcatcaag
      901 accgtccccg agtcgcagat cgtcgagacc ctcatcgagg aggcgatgcg cctcgccgag
      961 gactacgacg gcgcgagcgg ggagccgtcg gtcggcgtgt cctgaccggt ctacgctcct
     1021 ctcgtgggga agcggacgcc cgagggcggg cggcggcgcg cgtgctgagg acgacagcgc
     1081 cggtgcgcct gctcgagcgc cgcgacaccg acgaggtgct cgacctgctc gaccgcgacc
     1141 cggtcgccga cgtcttcgtc gcctcacggg tccgcggtgc gggcctggcc gagagccggc
     1201 tcggagcgca ggtgtggggc tacggcgagc ggggccggct cgaggcgctg tgctactccg
     1261 gcgccaacct cgtcccggtg caggccggac cggaggccgt ccgggcgttc gccgagcggg
     1321 cccgccgcca gggccgccgg tgctcgtcca tcgtcggccc cgccgacgcg gtcggcgagc
     1381 tgtggcggct gctcgagtcc agctggggcc cggcccgcga ggtccgcgac tgccagccgc
     1441 tgctggcgat cgacggcccg cccgcgctgc ccgccgaccc ccgggtgcgg ccggtccgcc
     1501 cggacgagct cgacgtgctg ctgcccgcct gcgtcgcgat gttcaccgag gaggtgggcg
     1561 tcagcccgac ggcgggcgac ggcggcgctc tctaccgcgc gcgggtgcgc gagctcatcg
     1621 cgcagggccg ggcgtacgcg cggatcgagg acggccgggt gctgttcaag gccgaggtcg
     1681 gcgcggcgac cgcgacgacc tgccaggtgc agggcgtgtg ggtcgacccg gcgctgcgcg
     1741 ggcgcgggct gggcaccgcc ggcacg
//
LOCUS       DASWNJ010001240         2874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_127246, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001240 DASWNJ010000000
VERSION     DASWNJ010001240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127246"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VGB85_20900"
     CDS             <1..326
                     /locus_tag="VGB85_20900"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HEY0136560.1"
                     /translation="ERFQSPDGPPVMLVSLRAGGTGLNLTAADHVYLLDPWWNPAVED
                     QAADRTHRIGQQRPVFVHRLVAEGTVEEKILELHQRKRALAGAVIEGAAAPALTREDL
                     IELLR"
     gene            complement(329..>2874)
                     /locus_tag="VGB85_20905"
     CDS             complement(329..>2874)
                     /locus_tag="VGB85_20905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136561.1"
                     /translation="GRVQAVKANHDVPAWLHRVVMRGLQPDPASRWPSMDALLVALQP
                     PSSKRWIWTTVIAGSTSALLLLGGMGYVFMMSSPPPPRADRGEEAAAAAEQVRVEADL
                     LRAQDEKALTEARAASTRDPVETVRALAKLAGDDPATWQDARFLAAVAQARGLPEQVL
                     RAGDRSLAEVWPLAGGGFVGRDDLGAVWQWKLSEATGTQILPAGAAAQLLVARDVPVW
                     AVMNGKSIQVFAADQAQKIDLDEATVLASDWRLASDGRTLTATTWMQGTDLGSGGTVY
                     LWDLTQPGTKPRTIAVPASTHAVIADDAAVVVTKELKNIRVTRPREGAENTLKYGGTP
                     WALSADGRFVIARPVDKDGVMDVVELETGKTRRVEAEGVTVLAGADVLFMQTVYTRPQ
                     YRRESLATGEVAWRLATPAQAGYDDRLIVDPANGQFAIALGEQWGIGDLVRGDLTSYM
                     TVPKDRRPQWAGPGLLMVSTGSELRIHRPAHAPVQMRHDGSGCALAPGGRWAVVQPWD
                     LERGEYTRVDLTTKQTATFRCPSTPKPVDMGGDSLMHFVSSAIDGAGQVTLMGADGWS
                     CWYDDLHGARAGAQSLKGGHLVGLPRGTAITDGAEVELWSGPEERTQRWTAAGAVMDL
                     AASPSGALLAVRSDRGVQLLRVDTGEVVPVITQATPVGLRDVIAGALAWSPDGTRLAT
                     LDKVDGALELIVWELAAVPREVSRHGFKDSPVRRRNWLEFTPSGAAVALSDRNKSAML
                     LPLGGQPMQRLAVPELYALHMRSETDAIGIDFEGLPVMIDFAAAEVAGLTPNPEIGLS
                     TSPPMQRGEDGSLWTCASLGPGALVEHAPQGVTPLADTREHVRKLASSL"
BASE COUNT          393 a         1114 c          963 g          404 t
ORIGIN      
        1 tcgagagatt ccagagcccg gacggcccgc cggtgatgct ggtgtcactg cgcgccggcg
       61 gcacggggct caacctcacc gccgccgatc acgtgtatct gctcgacccg tggtggaacc
      121 ccgccgtcga ggatcaggcg gccgaccgca cgcaccggat cggccagcag cgcccggtgt
      181 tcgtgcaccg cctggtcgcg gagggcaccg tcgaggagaa gatcctcgag ctgcaccagc
      241 gcaagcgcgc cctcgcgggc gcggtgatcg agggcgccgc cgccccggcg ctgacccgcg
      301 aggacctgat cgagctgctg cgctgagctc acagcgatga cgcgagcttg cggacgtgct
      361 cccgcgtgtc cgccagcggc gtcacgcctt gcggcgcgtg ctcgacgagc gcgccgggcc
      421 cgagcgaggc gcaggtccac agcgagccgt cctcgccccg ctgcatcggc gggctggtcg
      481 agagcccgat ctccgggttc ggcgtcagcc ctgcgacctc ggccgccgcg aagtcgatca
      541 tgaccggcag gccctcgaag tcgatcccga tcgcgtcggt ctcgctgcgc atgtgcagcg
      601 cgtacagctc cggcaccgcg agccgctgca tcggctgacc gccgaggggg agcaacatcg
      661 ccgacttgtt gcggtcactc agcgccaccg ccgcgcccga gggcgtgaac tcgagccagt
      721 tgcgccggcg aaccgggctg tccttgaagc catgtcggga gacctcgcgc ggcaccgcgg
      781 cgagctccca cacgatcagc tcgagcgcac catcgacctt gtcgagggtc gccagcctcg
      841 tgccgtcggg gctccacgcc agcgcacccg cgatcacgtc gcgcagaccc accggcgtcg
      901 cctgcgtgat caccgggacg acctcgccgg tgtcgacgcg cagcagctgc acgccccggt
      961 cgctgcgcac cgccaacagc gcgcccgacg ggctcgccgc caggtccatc accgcgcccg
     1021 cggccgtcca gcgctgcgtc cgctcctccg gtcccgacca cagctcgacc tccgcgccgt
     1081 cggtgatggc ggttccgcgt ggcagcccga cgagatggcc gcccttgagc gactgcgcgc
     1141 cggcgcgcgc gccgtgcaga tcgtcgtacc agcagctcca tccgtccgct cccatcaggg
     1201 tgacctgtcc cgcgccgtcg atcgccgagg atacgaagtg catcaaggag tcaccaccca
     1261 tgtcgaccgg cttcggcgtg ctcgggcagc gaaacgtcgc cgtctgcttc gtcgtaaggt
     1321 cgacccgtgt gtactccccg cgctcgaggt cccacggctg cacgaccgcc cagcggccgc
     1381 cgggggccag cgcgcagccg gagccgtcgt ggcgcatctg taccggcgca tgcgcgggcc
     1441 gatggatccg cagctcgctg ccggtcgaca ccatcaacag gcccggcccg gcccactgcg
     1501 gtcgcctgtc cttcgggaca gtcatgtacg aggtcagatc accgcgcacg aggtcgccga
     1561 tgccccactg ctcgccgagc gcgatcgcga attgaccgtt ggccgggtcg acgatcagcc
     1621 ggtcgtcata gccggcttgc gccggcgtcg ccagccgcca cgcgacctcg ccggtcgcca
     1681 gcgactcgcg ccggtactgc ggacgcgtat agaccgtctg catgaacagc acgtcggcgc
     1741 ccgcgagcac tgtcaccccc tcggcctcga cgcgccgcgt cttgccggtc tcgagctcga
     1801 ccacgtccat gacgccgtcc ttgtcgactg gccgtgcgat gacgaagcgg ccgtcggcag
     1861 acagcgccca gggcgtgccg ccgtacttga gcgtgttctc ggcgccctcg cgcggccgcg
     1921 tcacccggat attcttcaat tctttggtga ccaccaccgc ggcatcgtcg gcgatgaccg
     1981 catgcgtgct cgctggcacc gcgatcgtcc gcggcttggt tccgggctgc gtcaggtccc
     2041 acaggtacac cgtgccgccg gatcccaggt cggtcccttg catccacgtc gtcgcggtca
     2101 gcgtgcgccc gtcggacgcc agccgccagt cgctggcgag caccgtcgcc tcgtcgaggt
     2161 cgatcttctg cgcctggtcg gccgcgaaca cctggatcga cttgccgttc atcacggccc
     2221 acaccggcac gtcgcgcgcg acgagcagct gggccgcggc gcccgcgggc aatatctgcg
     2281 tacctgtcgc ttcggacagc ttccattgcc acaccgcgcc gagatcgtcg cggccgacga
     2341 agccgccgcc cgcgagcggc cacacctcgg cgagcgaccg atcgccggcg cgcaggacct
     2401 gctccgggag cccacgcgcc tgcgcgacgg cggcgaggaa gcgcgcgtcc tgccaggtcg
     2461 ccgggtcgtc gccggccagc ttcgccagcg cgcggaccgt ctcgaccggg tcacgcgtcg
     2521 atgcggcgcg cgcctcggtc agcgccttct cgtcctgcgc ccgcagcagg tcggcctcga
     2581 cgcgcacctg ctcggccgcg gcggcggcct cctcgccccg atctgcgcgc gggggagggg
     2641 gtgagctcat catgaacacg tagcccatgc caccgagcag cagcagcgcg gacgtcgagc
     2701 ccgcgatcac cgtggtccag atccagcgct tcgagctcgg gggctgcagc gcgaccagca
     2761 gcgcgtccat cgacggccag cgcgacgcgg ggtccggctg caggccgcgc atcacgacgc
     2821 ggtgcagcca cgccggcaca tcatgattcg ccttgaccgc ctgcacgcgc cccg
//
LOCUS       DASWNJ010001241         2263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_305963, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001241 DASWNJ010000000
VERSION     DASWNJ010001241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305963"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1167
                     /locus_tag="VGB85_20910"
     CDS             97..1167
                     /locus_tag="VGB85_20910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136562.1"
                     /translation="MAVVVGLLGSATACQAHVLSHSTGESSSSGDDGDPEPDPPVPTS
                     DPDPTAAPTATGEVDTTSGTSGTSDTGDATSTTTDPSGVCGDGVVDAGETCDLGFAGN
                     SDEGSCTQACQTAYCGDGLVWVGEEICDNGANNNDQTYNGCQRACTPGPRCGDGALQI
                     EEECDPSVMQDEVVVSCDPDTCRFNARVAFVTSASFAGNFGGLTGADAACVAAAQTAG
                     LDNASNFMAWVSDGVTGPAKRFTLVPGYPYTRRDGQPIAYDLDTMYEEGLQRPLDITE
                     LGTTLPPGEYAWSNTKVNGQPLDPQDHCQAWQSDSSQQLGRAGQVSPLPADFATWIQS
                     AQWTQYTASLCKDSCHLYCFEQ"
     gene            1180..2046
                     /locus_tag="VGB85_20915"
     CDS             1180..2046
                     /locus_tag="VGB85_20915"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0136563.1"
                     /translation="MTRVFLSVSFLLSGCGVEELAAILAKYDTFGESSSSGELDTGAA
                     GSSTGGGEPGESGTDSTGGPAPASTTSTNSGTGGLDTGGETTAGPGSTGPAGPVCGDG
                     VVEGEEECDDANTIDGDGCYNNCPRSWLVFVTSEPRTQGGLKGLIGADYQCRHRASKL
                     FLPNGDRYMAWVSTSEVQPADRMYHARGPYRLVNGDQVAANWDALVAGPLDHPIMLDE
                     LGEVVNALVFTGTTPEGWRVPNSTRCDDWTDSSGKNFTWYGDSTATDKAWTFAAESHC
                     GAHAALYCFEQP"
BASE COUNT          395 a          760 c          757 g          351 t
ORIGIN      
        1 aaatgtatgc gccattacat ttaattcatc tgtcggccga tcttgacatg gggtagcgat
       61 atcgcgcatc ctcccggtgt gcaaagacaa cgcgtcgtgg ccgtggtcgt cgggttgctc
      121 gggtcggcga cagcctgcca ggcgcacgtg ttgtcccact cgaccggcga gtccagctcg
      181 agcggcgacg acggcgaccc cgaaccggac ccgcccgtgc cgacctcgga cccggacccc
      241 accgctgctc ccaccgcgac cggggaagtc gacaccacgt ccgggacgtc cggcacgagt
      301 gacaccggcg acgcgacgag cacgaccacg gacccgtccg gggtctgcgg cgacggcgtg
      361 gtcgacgccg gggaaacttg tgacctgggg tttgcaggca acagtgatga gggctcctgc
      421 acccaggcct gccagacggc gtattgtggc gatgggttgg tctgggtcgg cgaggagatc
      481 tgcgataatg gagccaataa taacgaccaa acctataacg gatgccagcg agcgtgtacg
      541 ccagggccca ggtgcggtga tggcgcgttg cagatcgagg aagaatgcga tccgagcgtg
      601 atgcaggacg aggtcgtcgt ctcctgtgac cccgatacct gccgattcaa cgcgagagtt
      661 gcttttgtta catccgctag ctttgccggt aatttcgggg gcctcaccgg ggccgatgcg
      721 gcctgtgtgg ccgcggccca gaccgccggc ctcgacaacg ccagcaactt catggcgtgg
      781 gtcagcgacg gcgtgaccgg acccgcgaag cgcttcaccc tggtgccagg ctacccctac
      841 acccgccgcg acggccagcc gatcgcctac gacctcgaca ccatgtacga ggaggggttg
      901 cagcgcccgc tcgacatcac ggagctcggc accaccctgc cgcccgggga atacgcctgg
      961 tccaacacca aggtgaatgg ccagccgctc gacccgcaag atcactgcca agcctggcag
     1021 tcggattcct cgcaacaact ggggcgggcc gggcaagtct cgccgctccc ggcggatttt
     1081 gcgacgtgga tccagtccgc gcaatggacg cagtacactg cgagcctctg caaagatagc
     1141 tgccatctct actgtttcga gcagtgaccc ggagccccca tgacccgcgt tttcctttca
     1201 gtctcgttcc tcctctccgg gtgcggcgtc gaggagctcg cagcgatcct cgccaagtac
     1261 gacacgttcg gggagtcgag cagctcgggc gagctcgaca ccggggcagc cgggagctcg
     1321 accggcggag gcgagccggg cgagtccggg accgatagca cgggcgggcc cgcaccggcg
     1381 tcgaccacgt ccacgaatag cggcactggc ggcctcgaca cgggcggcga gacaacggcc
     1441 gggcccggca gcacaggccc ggccgggccg gtgtgcggtg acggggtggt cgagggcgag
     1501 gaggagtgcg acgacgccaa cacgatcgac ggcgacggtt gctacaacaa ctgcccgcgt
     1561 tcgtggctgg tgttcgtgac cagcgagccg cggacccagg gcggcctcaa ggggctgatc
     1621 ggggccgatt atcagtgccg gcaccgggcg agcaagctgt tcttgcccaa cggcgaccgc
     1681 tacatggcgt gggtcagcac cagcgaggtc cagccggccg accgcatgta ccacgcccgg
     1741 ggcccgtacc gcctcgtcaa cggcgaccag gtggccgcca actgggacgc gctggtcgcc
     1801 ggcccgctcg accacccgat catgctcgac gagctgggcg aggtcgtcaa cgccctggtg
     1861 ttcacgggca ccacgcccga gggctggcgc gtgcccaact cgacccgctg cgacgactgg
     1921 accgactcca gcggaaagaa cttcacctgg tacggcgact cgacggcgac cgataaggcc
     1981 tggaccttcg ccgccgaatc tcactgtggt gcccacgccg cgctgtactg cttcgagcag
     2041 ccgtgatcat ggtctgccgc cgtccagggt gagtcgggag agtctcggct ctcccggctg
     2101 atgatgcctc acggatcgag agcgaccccg gagcccgcat gacacgcgtt ttcctttcag
     2161 tctcgtttct cctctccggg tgcggcgtcg aggagctcgc agcgatcctc gccaagtacg
     2221 acacgttcgg ggagtcgagc agctcgggcg agctcgacac cgg
//
LOCUS       DASWNJ010001242         2376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_374198, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001242 DASWNJ010000000
VERSION     DASWNJ010001242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374198"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..2241)
                     /locus_tag="VGB85_20920"
     CDS             complement(172..2241)
                     /locus_tag="VGB85_20920"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY0136564.1"
                     /translation="MTARDPSISAIFQPDPSAPVRALLVAGSCGNVGFGKLGQFARVL
                     ARHGVPVIALDLSADVLTVRERLAKEFSARLGADEIAAITAGITPIQGTLADIPADLA
                     IGMVFEAIPERLSIKRPFYEAVRARDPEAYIFSATSGLTTRKLFEGLAGSERCGVLHP
                     FFPHLTNKLWELPEHGAVTGKAEAKAIKSFLGRAGMNLISVRDVPSFAADRLFCGMML
                     EAVRIHADTGLTPAQIDDVCKQVLGTSPFFVHNLIRGANGLSAHCMELMQEEVDSSLY
                     SIPEVWQPYIVDPGKQWPYERGQRCPPEAVQGVADRMLGMLFALTAYMLKHDIAGADA
                     LNFLAEKALAFREGPPAIIEGMGLEPAADLTRRFLDQQRITKADEVAPLEVFQKTGPG
                     ADVGWHSVYVGRSVMNGVGLLSLKRSTLNFTFVAEIDRAYEALQRDPDVKAIVLAPDG
                     TYSREFGHGADLQCFVPVLGDKAGALKLIAQWKTTLGKLRAGKPTVAALVGRVLGGSL
                     ELATSCHARIAAEGTVLGQPEATVGVLPGLGGCHQIHRLSTPDAHARINEILLTGGNF
                     TAEEAVQWGFVSKVVPVKDLPRASMALAAEIADSGTLPAFRSEPLEVTVDPAIAGRNS
                     AGVALDRKHRELLVRTIEDCNNLPYAVAADVEAERAAESFALSSSKIGVQALLRGKPP
                     VFEAPLE"
BASE COUNT          340 a          787 c          910 g          339 t
ORIGIN      
        1 gtcgaagccg gcctcgccgg gcgaggtgac gccctcctgg ctctcctgca cgccctcacg
       61 cgcgagcagc cgcgcgagca cctgcgccgg cgggatcgcg agcgcgtaga cgagggtcga
      121 ggggccgccg cgcacggcca gcgcgtgacg gtccgtcggc atcgtggcgg gttactcgag
      181 cggagcctcg aacaccggcg gcttgccgcg cagcagcgcc tgcacgccga tcttgctgct
      241 cgagagggcg aagctctcgg cggcgcgctc ggcctcgacg tcggcggcga cggcgtaggg
      301 caggttgttg cagtcctcga tcgtgcgcac gagcagctcg cggtgcttgc ggtcgagggc
      361 gacgcccgcc gaattgcggc cggcgatcgc ggggtcgacg gtgacctcga ggggctcgct
      421 gcgaaacgcc ggcagggtgc cggagtcggc gatctcggcg gccagcgcca tgctggcgcg
      481 cgggaggtcc ttgaccggca cgaccttgct gacgaagccc cactgcacgg cctcctcggc
      541 ggtgaagttg ccgccggtca gcaggatctc gttgatgcgc gcgtgggcgt cgggggtcga
      601 gaggcggtgg atctgatgac aaccaccgag gccgggcagc acgccgacgg tcgcctcggg
      661 ctggccgagc acggtgccct cggcggcgat gcgggcgtgg caggaggtcg cgagctcgag
      721 gctgccgccg agcacgcgac cgacgagcgc ggcgacggtc ggcttgccgg cgcgtagttt
      781 gccgagcgtg gtcttccact gcgcgatcag cttgagcgcg ccggccttgt cgccgagcac
      841 gggcacgaag cactgcaggt ccgcaccgtg gccgaattcg cgggagtatg tcccgtccgg
      901 cgcgagcacg atcgccttga cgtcggggtc gcgctgcagg gcctcgtagg cgcggtcgat
      961 ctcggcgaca aaggtgaagt tgagggtgct gcgcttgagc gagagcagac cgacgccgtt
     1021 catgacgctg cgcccgacat acaccgagtg ccagccgacg tcggcgccgg ggccggtctt
     1081 ctggaacacc tcgagcggcg cgacctcgtc ggccttcgtg atccgctgct ggtcgaggaa
     1141 gcgacgggtc aggtcggcgg cgggctcgag gcccatgccc tcgatgatcg ccggcggacc
     1201 ctcgcggaac gcgagcgcct tctcggcgag gaagttgagc gcgtcggcgc cggcgatgtc
     1261 gtgcttgagc atgtaggcgg tcagcgcgaa cagcatgccg agcatgcggt cggcgacgcc
     1321 ctgcacggcc tcgggcgggc accgctggcc gcgctcgtac ggccactgct tgccggggtc
     1381 gacgatgtac ggctgccaca cctcggggat gctgtagagg ctgctgtcga cctcctcctg
     1441 catcagctcc atgcaatgcg cggacaggcc gttggcgccg cggatcaggt tgtggacgaa
     1501 gaacggggag gtgccgagca cctgcttgca gacatcgtcg atctgcgcgg gcgtcaggcc
     1561 ggtgtcggcg tggatccgca cggcctcgag catcatgccg cagaagaggc ggtcggccgc
     1621 gaagctcggc acgtcgcgca cggagatcag gttcatgccg gcgcgcccga ggaacgactt
     1681 gatcgccttc gcctcggcct tgccggtgac ggcgccgtgc tcgggcagct cccagagctt
     1741 gttggtcagg tgggggaaga acgggtgcaa cacgccgcag cgctcgctgc ccgccagacc
     1801 ctcgaacagc ttgcgcgtgg tcaagccgga ggtcgcggag aagatgtacg cctccgggtc
     1861 gcgggcccgc acggcctcgt aaaagggacg cttgatcgag agccgctcgg ggatcgcctc
     1921 gaagaccatg ccgatcgcca ggtccgccgg gatgtcggcg agcgtgccct ggatcggggt
     1981 gatgccggcc gtgatcgcgg cgatctcgtc ggcgccgagg cgcgcggaga actccttggc
     2041 gaggcgctcg cgcacggtca acacgtcggc cgagaggtcg agggcgatca ccggcacgcc
     2101 gtgccgcgcg agcacgcgcg cgaactgccc gagcttgccg aagccgacgt tgccgcagct
     2161 gcccgccacc aggagcgccc gcaccggggc ggagggatcg ggctggaaga tcgcggagat
     2221 cgaagggtcg cgggccgtca tcgcggccga gcataggggg tcgtggcccc gtccccacga
     2281 cgaccaactg gagctttccc acgggacggc gcccacagcg gcccctgggc gggttctggt
     2341 gccccccagt aggtttgggg tcggcgctga gcgcgc
//
LOCUS       DASWNJ010001243         1959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_514905, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001243 DASWNJ010000000
VERSION     DASWNJ010001243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514905"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            628..996
                     /locus_tag="VGB85_20925"
     CDS             628..996
                     /locus_tag="VGB85_20925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136565.1"
                     /translation="MSLNSRVLKVAALAAVVLIPAVAHADTYGIAGTVISLTINESSA
                     DNYLGERGSLILSEGGVPRKYQWGGTACNGRNVTEANVALLFRAMEHGDRMTVLPSYK
                     AGAGQARCLVGFTFTPVMTP"
     gene            complement(1017..1445)
                     /locus_tag="VGB85_20930"
     CDS             complement(1017..1445)
                     /locus_tag="VGB85_20930"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEY0136566.1"
                     /translation="MTDHDRAGCKSVELGFCVDALAGFPGPYTKYVLTTLGVAGLLRL
                     IDPLAARSYRFVAALVHVDGEGVAHTFLDDRAAGQLALEADGTPCAGAWSALWSVFIP
                     EGATRPLVALSTTEREALWTVCRAHSVYAQFVRWLSAAQR"
     gene            complement(1570..>1959)
                     /locus_tag="VGB85_20935"
     CDS             complement(1570..>1959)
                     /locus_tag="VGB85_20935"
                     /inference="COORDINATES: protein motif:HMM:NF024930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RCC1 domain-containing protein"
                     /protein_id="HEY0136567.1"
                     /translation="EGAIRCTGTGILSAQLADGSSPPVQLHQCPAEGLTGVKQMVDPC
                     ALMNDGTVRCWAGLSPPVAGGARTGKPTAIEGVTDVVALDVGASHACALSTGGAVSCW
                     GYNTQGQIDGKPSETFVWPPRRIDLPR"
BASE COUNT          321 a          655 c          691 g          292 t
ORIGIN      
        1 tgtgtagcgc tcgagcagcc gctgggcgct ctcctgcgtg ctggcgacgg tgcattccat
       61 cacagcaccc tagcagaggg gtcgcgcttg cagaagactc ctgagcggcc cgcagcgcca
      121 caaaaccgcg cgctttgggc cgctgcaccg cgcaatcgtt gctggcctgc acggggtgcc
      181 ggcgcgtaat ccacggaccc gagccgcacc tacctgtagc tcgagctcac cgagcgcggc
      241 gcgtgactct cccgtgcgta cgaccgtttt ggtgaagcag gacttgagtg cggagcgaga
      301 ttggcgtgag cgaggaagct cgacaggtcg aggccgacga taagcgtgaa cccacgagct
      361 gccaccagcc ccgagcagct caccgacctc gaagctgtcg ccgaggacgg cgcgtcgacc
      421 gaggtctgcg ctccgtcgca taaggaaggg tgcgtcatcc atgagggctg gtgagctgcc
      481 gcggcccggc gaagtcggca gcgccggggc gcggaaccga tgtgctgccg gtgaaggcgt
      541 atgcataatt tcgcaggaaa attgcgaggc cgggtctgtt aatgcagact gcgcgacggg
      601 ctctgaggcc cgcttccgag gaataccatg tcgcttaatt cacgagttct caaagttgcc
      661 gccctcgcgg cggttgtgtt gatcccggcg gtcgcccacg ccgacaccta cggcatcgcc
      721 gggacggtga tttcgttgac catcaacgag tcctccgccg ataactatct gggcgagcgc
      781 gggagcctca tcctgtccga gggtggggtg ccgcggaagt accagtgggg cggaacggcc
      841 tgcaacggtc gtaacgtcac cgaagcgaac gtcgccctgc tgttccgcgc gatggagcac
      901 ggggatcgca tgacggtcct gccgagctac aaggccggcg ccgggcaggc gcgctgtctg
      961 gtgggcttca ccttcacgcc ggtcatgacc ccctgaaaaa cgtcgcgcct gggccttcat
     1021 cgctgggcgg ccgagagcca ccggacaaat tgcgcatata ccgagtgggc gcggcaaact
     1081 gtccataatg cctcacgctc ggtcgtcgat agcgcgacga gcggccgcgt ggcgccctcg
     1141 gggatgaaga cgctccatag cgccgaccag gccccggcgc agggcgtgcc gtcggcttcg
     1201 agcgccagct gccccgcggc ccggtcgtcg aggaaggtgt gcgcgacgcc ctcgccgtcg
     1261 acgtggacca gcgccgcgac gaaccggtag ctgcgcgcgg cgagcgggtc gatgagccgc
     1321 agcaggccgg cgacgccgag cgtcgtcagc acatacttgg tgtaggggcc ggggaagccg
     1381 gcgagcgcgt cgacgcaaaa ccccagctcg acgctcttgc agcccgcccg atcgtggtcg
     1441 gtcacgcagg ccccgttcgc ccggcacgcg gcccacaggg ctaaaagatc ggcgcgcatg
     1501 ccggggtgac gctcgagggt caagagcgcc ggctcgcgcg cgagatccga cgcactcgga
     1561 tctcagcgat cagcgcggca ggtcgatccg ccgcggcggc cagacaaagg tctcgctcgg
     1621 cttgccgtcg atctgcccct gggtgttgta gccccagcag ctgaccgcgc cacccgtgga
     1681 cagcgcgcag gcgtggctgg ctccgacgtc gagggcgacg acgtcggtga caccctcgat
     1741 cgccgtcggc tttccggtcc gcgcaccgcc ggcgacggga ggggacagac cggcccagca
     1801 gcgcacggtg ccgtcattca tgagcgcgca aggatcgacc atctgcttca cgcccgtgag
     1861 gccctcggcc gggcactggt gcagctggac cggcggcgag ctgccgtcgg cgagctgggc
     1921 gctgaggatg ccggtgccgg tgcagcggat cgcgccctc
//
LOCUS       DASWNJ010001244         7695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_404981, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001244 DASWNJ010000000
VERSION     DASWNJ010001244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7695
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404981"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VGB85_20940"
     CDS             <1..352
                     /locus_tag="VGB85_20940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136568.1"
                     /translation="HLARALTIRTRALGPNHPDVAQCLAAQSELERLAGGDATTPAER
                     ALQIFEAHPSTLKEDIYEARLRLARALAARKPPDTARARTLAEAARDGFAALAPADFA
                     SRRADAEAFLASLR"
     gene            complement(357..1412)
                     /locus_tag="VGB85_20945"
     CDS             complement(357..1412)
                     /locus_tag="VGB85_20945"
                     /inference="COORDINATES: protein motif:HMM:NF039450.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADYC domain-containing protein"
                     /protein_id="HEY0136569.1"
                     /translation="MKNLTSTHSFLALALTTLMSAGCDEVAQDEAFGEGDVSERCVNC
                     GGGILFNTFMWGKEDGGSLDVKGAVWSNARLVSVELACPKSEKDLWRFPKECLKDTMF
                     RLDAVRAVSGELLGKRDIFDFGGADFLGSRWTVDLYDGEKVVGTHVQNITAYAYDATQ
                     KPHALHYYTFMFYGDGANGGEKGVWTPACKESVDPITGLPVGTKAVVYDDIEVDTKTG
                     KISERESSLYLACITGAVGKAGNWGYPAWEIGHEDFTTSVRMVRADYCGDGVSWTTIG
                     SSLQAKDVFKYRDFSNANGATEAMWGEKGALCLGTPRWTTQYAYSDVSCNGAKLQPCG
                     AATLTDYSEAIAWTKLP"
     gene            1806..2423
                     /locus_tag="VGB85_20950"
     CDS             1806..2423
                     /locus_tag="VGB85_20950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0136570.1"
                     /translation="MDDQELLSAWRQGDEDAGEALFERFYDSLSRFFRNKAGEHAPDL
                     IQRTFLVLLETQSRMREGTTFQAYLFGVARNILLEHYRGKRRDGERFDQAEQSVADLG
                     PTPTTLLARAQESQLLLQALRSIPIESQIILELYYWENMTARDIADVLEIPEGTARTR
                     IRRAKQLLETQLATLAATPALLQSTVSDLDSWARRLRDDLKPPPG"
     gene            2517..5738
                     /locus_tag="VGB85_20955"
     CDS             2517..5738
                     /locus_tag="VGB85_20955"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0136571.1"
                     /translation="MTDEPQAPTGDRALASSLALARTHTPLSPALAQTLTPLPLELDQ
                     TRTPTPDDPRPGDSMSALLAPTLLSNDDGASHLALTPPEDDDHRAKQLLKARLFRGKA
                     TPVKIGRFTILDRLGEGGMGVVYTAYDDQLDRKIAVKVLRGEATRRDDSGRTRLLREA
                     QAMARLSHPNIVTVHEVGALGDQIYIAMEFVRGLSLDAWLKQQQVRPWREVLDTFIKA
                     GRGLEAAHRAGIIHRDFKPHNVLVGDEGAVKVLDFGLARAVEHAGSEELSTTPESGAY
                     NHDGNLLDTPLTRTGAIMGTPAYMAPEQHEGRTATAASDQFSFCVSLHDGLYGVHPFD
                     CSNLGALIAGVTSGRVLEPVSHARVPGWLRRVLLRGLAVDPDSRWPSMTALLAELAKD
                     PAQTRRRWLATAGLIGFVGAGSFGAATLLPAAQAVCQGIDAELADIWDPARSSAVQAA
                     ISSTGLAYAGDTWARVQPGLDAYARSWLAMRAEACETHHAARQSDQLFDLRTACLDQR
                     RDSLDALVDILADADAVAVEKAVTAVAALPSIAACNDTDALTQAVPPPEDPALRERVA
                     QARSTLARVKAHEDAGQYAQALAALAPVLADAELATYKPLLAEALLRRGTLKMYTGDA
                     NGSHEDLAAAARAAVAAGHDNIAAQSVSRDLFVRAQLLHQASAALEDAGLTQAMVERV
                     ADDRPLYAEFLNNLGVAQLVADDFVHALESLTTSLEIKQQALGEEHPEVAYTLANMAG
                     LKVQANRFDEAVVDFQRALEIIQRSFGPAHPTAAAIEYNLAWALRLNGKPAQATARLR
                     HVLELHAANKTVAGLDQCHFDLGFAALALNDLTGAQHHFAAVSRTARDGDKLAGSYLH
                     EAQGDLHAARGDIISARQEYAASLAARIEILGADSLGVAHTHQRYGEMLLRAGQAPEA
                     LFELRAALAIHERSLSTDNPATAKTLELIGRAELAHGDLTAARTSLERALQIRQAALP
                     PTSLDIARSLRHLGELRLAADEHAAAVDLLTRALTSLTAGQAEPHELSATRFVLARAL
                     ASAGQRDAAVSAARQARDAFKSAGEHWQREAAEVDAWLASDPG"
     gene            complement(5839..7074)
                     /locus_tag="VGB85_20960"
     CDS             complement(5839..7074)
                     /locus_tag="VGB85_20960"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HEY0136572.1"
                     /translation="MATTFRIDYTRLLDLWRSIEQAGGRDKYITAQLKEKGYLLDRKP
                     TEGMSKAELDRYKKTLKEEAAERKRLAKEAWQAYKAAHLVHLGEGVFWNDDLDHDKWD
                     LPDAEERQAENELPRLDKPKQLAEALKLSIAELRVLAYHRDAATRLNYYRFTIPKRDG
                     SRRAIWAPHKRLKAAQRWLLREVVERLPVHGAAHGFMHGRSIASNAQVHTDAKTVLKV
                     DLKDFFPTVTLPRVKGVFRKAGYREQIATLLALLCTEAPRQISEVDGKQYYLALGPRC
                     LPQGAPTSPGLTNTLCMRLDRRLQGAAHKLGWRYTRYADDLTFSLPTHHKGEPKLGKL
                     LGTISAIAVGEGFVVHPDKTRVSRSGGRQRVTGLVVNGKGGPRVPREIKREIRAALHN
                     LSKGKPLRAELDKVQAAVG"
     gene            complement(7080..>7695)
                     /locus_tag="VGB85_20965"
     CDS             complement(7080..>7695)
                     /locus_tag="VGB85_20965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136573.1"
                     /translation="MYDLARGVYRLRPLTDAPIDLDRLQYRNVRERVAHDLVARGAVK
                     IASENRIYGTGLEVTGKVAVEADRREYRPQLVLDDDGRVKSAECTCTFYRKHKLKEGP
                     CAHLIALRVAQAQEEERRRQARGKARATITVETRTYAKRNGDSEEVVQLSLDRQRIKL
                     RWGPRDQGLRVQSLVFNSVNEARAAYFERVDQLEARGYLDGTAG"
BASE COUNT         1118 a         2980 c         2442 g         1155 t
ORIGIN      
        1 ccacctcgcc cgcgccctga cgatccgcac gcgcgcgctc ggacccaacc acccggacgt
       61 cgcccagtgc ctcgccgcgc agtcggagct cgagcgcctc gccggcggcg acgcgacgac
      121 gcccgccgag cgcgccttgc agatcttcga ggcccacccc tccacgctca aggaggatat
      181 ttatgaggcc cgtctgcgac tcgcccgggc cctcgccgct cgcaagccgc ccgacacggc
      241 gcgcgcccgg accctcgccg aggccgcgcg cgacggcttc gccgcgctcg cccctgccga
      301 cttcgccagc cgccgcgccg acgccgaggc cttcctggcg agcctgcgtt gacgcctcag
      361 ggcagcttgg tccaggcgat cgcctcggag tagtccgtca gggtcgcggc gccgcacggc
      421 tgaagcttgg cgccgttgca gctcacgtcg ctgtacgcat actgggtggt ccagcgcggg
      481 gtgccgaggc acagcgcgcc cttctcgccc cacatcgcct ccgtggcgcc gttggcgttg
      541 ctgaagtcgc gatacttgaa cacgtccttg gcctgcaacg agctgccgat cgtcgtccac
      601 gacaccccgt cgccgcagta gtcggcgcgg accatgcgga ccgaggtcgt gaagtcctcg
      661 tgcccgatct cccacgccgg gtagccccag ttgccggcct tgccgaccgc gccggtgatg
      721 cacgccaggt acagcgagct ctctcgctcg ctgatcttgc cggtcttggt gtcgacctcg
      781 atgtcgtcgt agacgacggc cttggtgccg accggcaggc cggtgatcgg gtcgacgctc
      841 tccttgcacg ccggcgtcca cacgcccttc tcgccgccgt tggccccgtc gccgtagaac
      901 atgaacgtgt agtagtgcag cgcgtgcggc ttctgcgtcg cgtcgtacgc gtacgccgtg
      961 atgttctgga cgtgcgtgcc gaccaccttc tcgccgtcgt acaggtcgac cgtccagcgc
     1021 gagcccagga agtccgcgcc gccgaagtcg aagatgtccc gcttgcccag cagctcgccc
     1081 gagacggccc gcaccgcgtc gagcctgaac atcgtgtcct tgagacactc cttcgggaac
     1141 cgccacaggt ccttctcgct cttggggcac gccagctcga cgctgaccag ccgcgcgttg
     1201 gaccacacag cccccttgac gtcgagcgag ccaccgtcct ccttgcccca catgaaggta
     1261 ttgaacaaga tgccgccacc gcagttgacg cagcgctcgc tgacatcgcc ctcgccaaag
     1321 gcctcgtcct gcgcgacctc gtcacagccg gcgctcatca gggtcgtcag agccagggca
     1381 agaaaagaat gggtgctggt aaggttcttc atggctgtcg ttcgctccac cgtgccgctt
     1441 cgaatcggta ggtgatcgtc gaccgccgaa caattgcggg gatcgcaaaa aaaccacggc
     1501 gcgttccagc gccgcggccc cgaacgggca gcgacgcgga tgcgcgcgga ctcacaggcc
     1561 tgtggagcgg ccagggtgct gcctcgcagc tcgtcgcgac gcgacacacg aggagccagg
     1621 gccagcctcc tcgtgtgtca tcaatcttcg cgtagtcgat cgcacggcct tcgggccctc
     1681 tcgggcagtg gtaccaggtc gccgaacaat tgcccgggcc cgcgaaaaat tcgcgggctg
     1741 cccgagcgag gtccgttccg ggcccgctcc ccgcgaccac gatccgggct aacatccgcc
     1801 ccgtgatgga cgatcaagag ctcctcagcg cctggcgcca gggtgacgag gacgccggag
     1861 aagcgctgtt cgagcgcttc tacgacagtt tatcgcggtt tttccgtaac aaggccggcg
     1921 agcacgcccc cgacctgatc cagaggacct tcctggtgct cctggagacg cagagccgga
     1981 tgcgcgaggg cacgaccttt caggcttacc tgttcggggt cgcccgtaac atcctgctcg
     2041 agcactaccg cggcaagcga cgcgacggcg agcgcttcga ccaggccgag caatcggtgg
     2101 ccgacctcgg gccgacgccg acgaccctgc tggcgcgcgc ccaggagtcc cagctgctgc
     2161 ttcaggccct gcgctcgatc ccgatcgagt cgcagatcat cctcgagctc tactactggg
     2221 agaacatgac ggcacgcgac atcgccgacg tgctcgagat ccccgaaggc acggcgcgca
     2281 cccgcatccg ccgggccaag cagctgctcg agacccagct cgccaccctc gctgccaccc
     2341 ccgcgctgct ccagagcacc gtctccgacc tcgacagctg ggcccgacga ctccgcgacg
     2401 acctcaagcc gccgcccggg tgaggcggct ttgatgtccc cttcgatctg cgcaattgtt
     2461 cgggcggcga gcgtcactcg cctcccagca tgttgcgtgc acttcggagg gtggccatga
     2521 cagacgaacc gcaagcgccg acaggcgacc gcgccctcgc ctcgtccctg gccctcgcgc
     2581 gcacccacac gccgctgagc ccggcgctgg cgcagacgct gacccccctg ccgctcgagc
     2641 tcgaccagac ccggacgccc acgcccgatg acccgcgacc cggcgactcc atgtctgcgc
     2701 tgctcgcccc gacccttctc agcaacgacg acggggcgag ccacctcgcg ctcacgcccc
     2761 ccgaggacga cgaccaccgc gccaagcagt tgctcaaggc ccgcctgttc cgcggcaagg
     2821 ccaccccggt caagatcggc cgcttcacga tcctcgaccg cctcggcgag ggcggcatgg
     2881 gcgtcgtcta caccgcctac gacgaccagc tcgaccgcaa gatcgccgtc aaggtgctgc
     2941 gcggcgaggc cacccgccgc gacgacagcg gccgcacccg gctgctgcgc gaggcccagg
     3001 cgatggcccg cctgtcgcac cccaacatcg tcaccgtcca cgaggtcggc gccctcgggg
     3061 accagatcta catcgccatg gagttcgtcc gcggcctcag cctcgacgcc tggctcaagc
     3121 agcagcaggt ccgaccctgg cgcgaggtcc tcgacacctt catcaaggcc gggcgcggcc
     3181 tcgaggccgc ccaccgcgcg ggcatcatcc accgcgactt caagccgcac aacgtgctcg
     3241 tcggcgacga gggggccgtc aaggtcctcg acttcggcct cgcccgcgcc gtcgagcacg
     3301 ccggcagcga ggagctgtcc accacccccg agagcggcgc ctacaaccac gacggcaatc
     3361 tgctcgacac gccgctgacc cgcaccggcg cgatcatggg cacgcccgcg tacatggccc
     3421 ccgagcagca cgagggccgc accgccaccg cggcgagcga ccagttcagc ttctgcgtca
     3481 gcctccacga cggcctctac ggcgtccacc ccttcgactg cagcaacctc ggggccctga
     3541 tcgccggcgt cacctccggg cgcgtcctgg agcccgtcag ccacgcgcgc gtgccggggt
     3601 ggctgcgtcg cgtgctgctg cgcggcctcg ccgtcgaccc cgacagccgc tggccctcga
     3661 tgaccgcgct gctcgccgag ctcgcaaaag accccgcgca gacccgccgg cgctggctcg
     3721 ccaccgcggg gctgatcggc ttcgtcggcg ccggcagctt cggcgccgcc acgctgctcc
     3781 cggccgcgca ggccgtatgt cagggcatcg acgccgagct cgccgacatc tgggatccgg
     3841 cgcgcagctc ggcggtccag gccgcgatct cgagcaccgg cctcgcctac gccggcgaca
     3901 cctgggcccg cgtgcagccc ggcctcgacg cctacgcacg atcctggctg gcgatgcgcg
     3961 cggaggcctg cgagacccac cacgcggccc gccagtccga ccagctgttc gacctgcgca
     4021 ccgcctgcct cgatcagcgg cgcgacagcc tcgacgcgct cgtcgacatc ctcgccgacg
     4081 ccgacgccgt cgccgtcgag aaggcggtca ccgccgtcgc cgcgctgccg tcgatcgccg
     4141 cgtgcaacga caccgacgcg ctgacccagg ccgtgccgcc gcccgaagac ccagccctgc
     4201 gcgagcgcgt cgcgcaggcc cgcagcaccc tcgcccgcgt caaggcccac gaggacgccg
     4261 gccagtacgc gcaggccctc gccgcgctcg cgccggtgct cgccgacgcc gaactcgcca
     4321 cctacaagcc gctgctcgcc gaggccctgc tgcgccgcgg cactttaaaa atgtacaccg
     4381 gcgacgcgaa cggctcgcac gaggacctcg ccgccgccgc gcgtgctgcg gtcgccgccg
     4441 ggcacgacaa catcgccgcg cagtcggtct cgcgcgacct gttcgtccgc gcccagctgt
     4501 tacatcaggc ctccgcggcg ctcgaggacg ccgggctgac gcaggcaatg gtcgagcgcg
     4561 tcgccgacga ccgcccgctg tacgccgagt tcctcaacaa cctcggcgtc gcccagctcg
     4621 tcgccgacga cttcgtccac gcgctcgagt cgctgaccac ctcgctcgag atcaagcagc
     4681 aggcgctcgg agaggagcac ccggaggtcg cgtacaccct cgcgaacatg gccgggctca
     4741 aggtgcaagc gaaccgcttc gacgaggctg tcgtcgactt ccaacgagcg ctcgagatca
     4801 ttcagcgcag cttcggtccg gcacacccga ccgcagccgc gatcgaatac aacctggcct
     4861 gggctctccg cctcaacggc aagcccgcac aggcgaccgc gcgactgcgc catgttctcg
     4921 agctccatgc agcaaacaag accgtggcgg gcctcgatca gtgccacttc gacctcggct
     4981 tcgctgccct cgcgctcaac gacctcacgg gggctcaaca ccacttcgcc gccgtgtccc
     5041 ggaccgctcg agatggcgac aagctggccg gctcttatct tcatgaagca cagggagacc
     5101 tgcacgcggc tcgcggggac atcatcagcg cacgccaaga atacgctgct tccctcgcgg
     5161 ctcgcatcga gatcctcggc gcggactccc ttggcgtcgc gcacacccac cagcgctacg
     5221 gcgagatgct gttacgcgcc gggcaagcgc ccgaagctct gttcgagctg cgggccgcgc
     5281 tggcgatcca cgagcggtcc ctgtctaccg acaaccccgc gaccgccaag acgctcgagc
     5341 tgatcggccg cgccgagctg gcccacggtg acctcaccgc cgcccgcacc tcgctcgagc
     5401 gcgccctcca gatccgtcag gccgcgctgc ctccgaccag cctcgacatc gcccgctcgc
     5461 tgcgtcacct cggcgagctg cgcctcgccg ccgacgagca cgccgccgcc gtcgacctgc
     5521 tcacgcgcgc gctcaccagc ctcaccgccg gccaggccga gccccacgag ctgtccgcga
     5581 cccgcttcgt cctcgcgcgc gccctcgcga gcgccggcca gcgcgacgcc gccgtctcgg
     5641 ccgcccgcca ggcccgcgac gcttttaaaa gtgcaggcga gcactggcag cgcgaggccg
     5701 ccgaggtcga cgcctggctg gcgagcgacc ccggctagcg cctgtccttc cggacaggcg
     5761 ccggtcgtgg ccgcatcggc gagcgcgtca tcctccccgc gcgactggct cggagatcgt
     5821 ctgtccttcc ggacaggctc agccgaccgc ggcttgcacc ttgtcgagct ccgcgcgcag
     5881 cggcttgccc tttgaaaggt tatgcagcgc ggcgcggatc tcccgcttga tctcccgcgg
     5941 cacccgcggc ccgcccttgc cgttgaccac caaccccgtg acccgctgac gtcccccgga
     6001 cctcgacacc ctggtcttgt cgggatgcac cacaaagccc tcgccgaccg cgatcgcgct
     6061 gatcgtcccc agcagcttgc cgagcttcgg ctcgcccttg tgatgggtcg gcaggctaaa
     6121 ggtcaggtcg tcggcgtatc gcgtgtagcg ccagccgagc ttgtgcgcgg cgccctgcag
     6181 gcgccggtcg aggcgcatgc acagcgtgtt cgtcaggccc gggctcgtcg gagcgccctg
     6241 cggcaggcac cgcggcccga gcgcgaggta gtactgcttg ccgtcgacct cggagatctg
     6301 gcgcggcgcc tcggtgcaca gcagcgccag cagcgtggcg atctgctcgc ggtagccggc
     6361 cttgcggaac acgcccttga cccgcggcag cgtcaccgtc gggaagaagt ccttgaggtc
     6421 gaccttgagc accgtcttcg cgtcggtgtg cacctgcgcg ttgctggcga tcgagcgccc
     6481 gtgcatgaac ccgtgcgccg ccccgtgcac cggcaggcgc tctaccactt cgcgcagcag
     6541 ccaccgctgc gccgccttga gccgcttgtg tggggcccag atcgcccggc gcgagccgtc
     6601 gcgcttgggg atcgtgaagc ggtagtagtt gaggcgcgtc gccgcgtcgc ggtggtacgc
     6661 gagcacccgc agctcggcga tgctgagctt gagcgcctcg gccagctgct tgggcttgtc
     6721 gagcctcggc agctcgttct ccgcctggcg ctcctcggcg tccggcaggt cccacttgtc
     6781 gtggtcgagg tcgtcgttcc aaaacacccc ctcgccgagg tgcaccaggt gcgccgcctt
     6841 gtaggcctgc caggcctcct tcgccagacg cttgcgctcg gccgcctcct ccttgagcgt
     6901 cttcttgtag cggtcgagct ccgccttgct catgccctcg gtcggcttgc ggtcgaggag
     6961 gtagcccttc tccttgagct gcgccgtgat gtacttgtcg cgcccgccag cctgctcgat
     7021 gctgcgccag aggtcgagga gtcgcgtgta gtcgatgcgg aaagtcgtgg ccatgtgcat
     7081 taccccgcgg tgccgtccag gtagccgcgc gcctcgagct ggtcgacgcg ttcaaagtag
     7141 gccgccctcg cctcgttgac cgagttgaag accaggctct gcacgcgcag gccctggtcg
     7201 cgcgggcccc agcgcagctt gatgcgctgg cggtcgagcg agagctgcac gacctcctcg
     7261 ctgtcgccgt tgcgcttggc gtaggtccgc gtctcgacgg tgatcgtcgc gcgggccttg
     7321 ccgcgggcct gccggcgccg ctcctcttcc tgcgcctgcg cgacgcgcaa ggcgatcaaa
     7381 tgggcgcagg ggccctcctt gagcttatgc ttgcgataga aggtgcaggt gcactcggcc
     7441 gacttcacgc ggccgtcgtc gtcgagcacc agctgcgggc gatactcgcg gcggtcggcc
     7501 tcgaccgcga ccttgccggt cacctcgagc ccggtcccgt agatccggtt ctcgctggcg
     7561 atcttgaccg cgccgcgggc gacgaggtca tgtgcgacgc gctcgcggac gttgcggtac
     7621 tgcaggcggt cgaggtcgat cggcgcgtcc gtcaacgggc gcaggcggta gaccccgcgg
     7681 gcgaggtcgt acatg
//
LOCUS       DASWNJ010001245         3006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_619693, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001245 DASWNJ010000000
VERSION     DASWNJ010001245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619693"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2193)
                     /locus_tag="VGB85_20970"
     CDS             complement(<1..2193)
                     /locus_tag="VGB85_20970"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0136574.1"
                     /translation="MLALVGAAVWLWTCRGDEPEADPGTTAVVRGAVGTGPRDRPELP
                     EDPRHARSAAISGTIRDPQGQPIAGARVCALAGSPGQSRADAQPICSEAGRDGHYQIA
                     GLLPVRHRVHAMAPGFVPTLHTHGEGIRRRDTVELRPGQQVTGVDITLAGGGVEIHGV
                     VYDLSGGPIEGARVNAGGGGAGMAIASSGPDGEFSLWVRPGRAIVWSDTEGYAQASSE
                     GVAPGHRFELYLTPESVVIGKVVRAGDGSPVADAEVWVERGDSNSMIMSGGGAVFTDA
                     GGNFRVEGLSPGAYKARAEADDAVGVAAEQVVLGLGETSEPIVIQAHPAFYAEGRIVV
                     IGGGDGCDDGSVHLKDAAHKREGFGQPGPDGELRARGLLPGTYAVTVHCPGHLLAESY
                     PPVVIGEAHVTGLRWEVERGQAIRGVVVDAAGKPAPGLDVGAFSRPDPSQPRTRQTTA
                     TQRSDEQGRFELAGLVPGAYQVTVNAWTMPRAVPAEPIDVTLPAGQDVDDLRIELPAA
                     GEVRGSVRDAKGQPVSQVQLQILTTRGTQTTRTADDGTFRFAEVPAGEHRLVASRAGN
                     LLRAPGASDDEPAERKVEVKVGGVVTVDLVVEGPAGRISGVVRDEGGAIVADAFVEAS
                     REPESAAAAAGEGARARWRGSLDRPRLTDADGRFTIDGLLPGKYAVRALRRGGGEALV
                     EHVALGEAVTLTIEATGRLAGTVSVDRGGVPAQFIVRIEDRRTGFLRRD"
     gene            complement(2410..2694)
                     /locus_tag="VGB85_20975"
     CDS             complement(2410..2694)
                     /locus_tag="VGB85_20975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136575.1"
                     /translation="MSPSLRLFAGLLAVSLVGCAGGGTPPTATPGAAYVVKRETPFYD
                     SGCAQDRKTDGKLKKGTRFTLVSEREGCWNIKLAGEDEVYIVPDRVAPAG"
BASE COUNT          386 a         1244 c          951 g          425 t
ORIGIN      
        1 gtcgcggcgc aaaaacccgg tcctgcggtc ctcgatgcgc acgatgaact gcgccggcac
       61 cccgccacgg tcgacgctca ccgtgccggc gagccgtccg gtcgcctcga tcgtcagcgt
      121 cactgcctcg ccgagcgcga cgtgctcgac gagcgcctca ccgccgccgc gtcgcagagc
      181 ccgcaccgca tacttgccgg gcagcagccc gtcgatcgtg aagcgcccgt cggcgtcggt
      241 caggcgcggc cgatcgagcg agccacgcca gcgcgcccgc gccccctcgc cggcggccgc
      301 cgccgcgctc tcgggctccc ggctggcctc gacgaatgca tcggcgacga tcgccccgcc
      361 ctcgtcgcgc acgaccccgg agatgcgccc cgcgggcccc tcgaccacga ggtcgaccgt
      421 gaccacgccg ccgaccttca cctcgacctt gcgctccgcc ggctcgtcgt cgctggcccc
      481 cggcgcgcgc agcaggttac cggcccgcga cgcgaccagc cggtgctcgc cggccggcac
      541 ttccgcgaac cggaacgtgc cgtcatccgc ggtccgcgtc gtctgcgtcc cgcgcgtcgt
      601 caggatctgc agctgcacct gcgagaccgg ctggcccttc gcgtcgcgca cgctgccacg
      661 cacctcgccg gccgccggca gctcgatccg caggtcgtcg acatcctgcc cggcgggcag
      721 cgtcacgtcg atcggctcgg ccgggaccgc ccgcggcatc gtccatgcat tgaccgtcac
      781 ctgatacgcg ccgggcacga gacccgcgag ctcgaagcgc ccctgctcgt cggaccgctg
      841 ggtggccgtg gtctggcgcg tacgcggctg tgatggatcc ggccgcgaga acgccccgac
      901 atcgagcccg ggcgccggct tccctgccgc gtcgacgacc acgccgcgga tcgcctgtcc
      961 gcgctcgacc tcccagcgca gcccggtcac gtgcgcctcg ccgatcacca ccggcggata
     1021 gctctcggcc agcaggtgac ccgggcagtg caccgtcacc gcataggttc caggcagcag
     1081 cccgcgcgcc cgcagctccc cgtccggccc cggctggccg aagccctcgc gcttgtgcgc
     1141 cgcgtctttc aaatgcaccg acccatcgtc gcagccgtcg ccgccgccga tgaccacgat
     1201 ccggccctcc gcataaaacg ccgggtgcgc ctggatgacg atcggctccg acgtctcgcc
     1261 gagcccgagc accacctgct cggccgcgac accgaccgcg tcgtcggcct ccgcgcgagc
     1321 tttgtaagcg ccgggcgaga gcccctcgac gcggaagtta ccgccggcgt cggtgaacac
     1381 cgcaccgccg ccgctcatga tcatgctgtt gctgtcgccg cgctcgaccc acacctccgc
     1441 gtcggcgacc ggcgagccgt cgcccgcgcg cacgaccttg ccgatgacga ccgactcggg
     1501 cgtcaggtac agctcgaagc gatgccccgg cgcgacgccc tcgctcgacg cctgcgcgta
     1561 gccctcggtg tccgaccaaa cgatcgcccg cccgggccgg acccacagcg agaactcgcc
     1621 gtccggcccc gagctggcga tcgccatccc ggccccgccc ccgccggcgt tgacccgcgc
     1681 gccctcgatc ggcccgccgc tgaggtcgta cacgaccccg tggatctcga cgccgccgcc
     1741 cgcgagcgtg atgtcgaccc cggtcacctg ctgtcccggc cgcagctcga ccgtgtcacg
     1801 ccggcggatc ccctcgccgt gcgtgtgcag cgtcggcacg aagccgggcg ccatcgcatg
     1861 cacgcggtgc cggaccggga gcagccctgc gatctggtag tgcccgtcgc gtccggcctc
     1921 gctgcagatc ggctgcgcgt cggcgcggct ctggcccggg ctccccgcga gcgcgcacac
     1981 cctggccccc gcgatcggct gcccctgcgg atcgcggatc gtccccgaga tcgccgccga
     2041 ccgggcgtgc cgcgggtcct ccggcagctc cggccgatcc ctcggcccgg tcccgaccgc
     2101 cccccggacg accgccgtcg tccccgggtc cgcctccggc tcgtcgcccc tgcatgtcca
     2161 cagccacacc gccgcgccga ccagcgcgag catcacgacc cagatcccag cgcgtgaacc
     2221 tcttgtctgc ggcaattctc gagacgctcc gtacctggcc ccggggacat gtcctccggg
     2281 acctggtcat tcgtacaggc ccgccacgaa cgacgggggt cgtcgtcggt acacgcaggc
     2341 gcgaggaaaa agttcctcgt ttgcggcccc gcgcatcgtg acacgcaggc gcccgcgtgt
     2401 gaagacccct cagccggccg gcgcgacccg gtccggtacg atgtaaacct cgtcctcgcc
     2461 ggcgagcttg atgttccagc agccctcgcg ctcgctgacc agcgtgaagc gggtgccctt
     2521 cttgagcttg ccgtcggtct tgcggtcctg cgcgcagccg ctgtcgtaaa acggcgtctc
     2581 acgcttgacc acataggccg ccccgggcgt ggcggtcggc ggcgtaccac ctccggcgca
     2641 gccgaccagc gacaccgcga gcaacccggc gaacagacga agtgatggcg acatgcggcg
     2701 acctcggccg cattgtacgt cctgcgcggg ccacagtgtg gccctccagg cccggtcgtc
     2761 acgatccgcg ttcatcctgc gatggtgctc cgctcgcgct cgcgctcacg ggcctctacc
     2821 ctgagcacga tcgtcatggc gccttcggcc gccgcttcgc cttgccgcgc cgcttgagca
     2881 gcggcttgct cgtcggcgcc gtcttcgtcg cctcgagctc ctccgccgac agccgcgagc
     2941 gcagctcgta cacccggatc ttcccgaagc gctttcgcat cacatactgc tcggcgaaga
     3001 tcttct
//
LOCUS       DASWNJ010001246         3445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_271394, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001246 DASWNJ010000000
VERSION     DASWNJ010001246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271394"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="VGB85_20980"
     CDS             complement(<1..808)
                     /locus_tag="VGB85_20980"
                     /inference="COORDINATES: protein motif:HMM:NF023425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050825 - ice binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ice-binding family protein"
                     /protein_id="HEY0136576.1"
                     /translation="MSLALVAGCSGDVNIDISNTDGSQGSTSAQTTGGTTQTTGGPGT
                     TDDLPTTGATSTSGLDTEQGGSQSASQSGSETTADPSSSGDPDTTSTGTTATTGELIE
                     TTGTAGECMPKVGGPAPVILGMPDDLGMPGAYAVLAKTAVSSVPGGSIDGGHVGVSPA
                     AASAITGFSLILDPSGEFATSTTVIPPYRVYAANYTVPTPALLTTAVLGMQAAYTDAA
                     SRVPSDQLDLEDGDIGGLTLEPGIYTWGSTVLIPEDVTLFGCEDDVWILQV"
     gene            complement(1104..2105)
                     /locus_tag="VGB85_20985"
     CDS             complement(1104..2105)
                     /locus_tag="VGB85_20985"
                     /inference="COORDINATES: protein motif:HMM:NF023425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050825 - ice binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ice-binding family protein"
                     /protein_id="HEY0136577.1"
                     /translation="MSLLLLAGCSDPMDLDGSDTAASQSTGTSQADDSDAAPTTGGAT
                     TTTGGTDGSADEGTTAATEQGGSQGSSQSGTGGPQDTGDSASSGTTGDTENATCMGKL
                     GPAPVVLGGPDDLAAPGAYAVLAKAAITNVPGTKIDGGHVGISPAAASSITGFALVLA
                     PPGVYATSPVLKAPFRVYAADYTVPTPILLTTAVLAMQAAYTDAAGRVPTDYLNLAGG
                     DLGGLTLAPGIYTWGSTVLITDDVTLEGCEDDVWIFQISGDLDVSTSKRVLLAGGAQA
                     KNVFWQIAGQATLHVGAHVEGILLAKTGITLQTKASLHGRAFAQTMVALDKNAVTGP"
     gene            2678..>3445
                     /locus_tag="VGB85_20990"
     CDS             2678..>3445
                     /locus_tag="VGB85_20990"
                     /inference="COORDINATES: protein motif:HMM:NF013765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136578.1"
                     /translation="MNAPHTSADTPVMRQFLDIKAGYPDAILMFRMGDFYEMFFDDAI
                     TVAPVLDIAVTSRDKGDVDRVPMAGVPYHAIGGYLRTLVERGFKVAICEQMETPEQAR
                     LRKGPKIVRREVVRVVTPGVLVDEEHLRSEEPNYLLAIVAEGVGTGAQSEGFGVAALD
                     VSCGEFFALRCVDGVSLRAALSRLAPREILVDAGLHGWLREALGPACPRLEGRDSKAL
                     PAEMVARVQHLRAESGGEPLLPSEARAAALTLAYAEET"
BASE COUNT          474 a         1093 c         1330 g          548 t
ORIGIN      
        1 cgacctgaag gatccacacg tcgtcctcgc agccaaagag cgtgacgtcc tccgggatca
       61 gcacggtgct gccccaggtg tagatgccgg gctcgagggt caggccgccg atgtcgccgt
      121 cctcgaggtc gagctggtcg ctcggcacgc ggctggccgc gtcggtgtag gcggcctgca
      181 tgccgagcac cgcggtcgtc agcagggcgg gggtcgggac cgtgtaattg gcggcgtaga
      241 cccggtacgg cgggatgacc gtggtcgagg tggcgaattc gccggaggga tcgaggatca
      301 gcgagaagcc ggtgatcgcg ctcgcagccg ccgggctgac gccgacgtgg ccgccgtcga
      361 tcgatccccc gggcacgctc gagaccgcgg tcttggcgag cacggcgtac gcgccgggca
      421 tgccgaggtc gtcaggcatg ccgaggatca ccggcgcggg accgccgacc ttgggcatac
      481 attcgccggc cgtcccggtg gtctcgatga gctcgccggt ggtggcggtg gtgccagtgc
      541 tggtcgtgtc ggggtcgccc gagctcgagg ggtccgcggt cgtctcggag ccggactgcg
      601 aggcgctctg gctgccgccc tgctcggtgt cgaggcccga cgtgctggtg gcgccggtgg
      661 tcggcaggtc gtcggtggtg cccggcccgc cggtggtctg cgtggtgcca ccggtggtct
      721 gcgcggaggt cgagccctgc gatccatcgg tgttgctgat gtcgatgttg acgtcgccgg
      781 agcaaccggc gacgagcgcc agggacatgg agatacgata tttcatgagc agatgtcttc
      841 ctgtcgccgg gcccgcaatg ggctgaccga cgacccgcta cgacgactaa tttcgcacga
      901 tcgcgccgac gacgagtcgg cgcgatcgac agggccccat tgtcgtcacg aaaggccgga
      961 tcacaagcgt ggccgcgaga aataaccaga ttctcgatcc gcgacgcgtg gtccaacgaa
     1021 acccacgctg gcaattcact tcaattcgcg gagcgcaatg tgggcagttt ttgccgcgcg
     1081 cgatcggctg gtgcaacgga cgttcatgga cccgtcaccg cgttcttgtc gagcgcgacc
     1141 atcgtctgtg cgaacgcgcg gccgtgcagc gaggccttgg tctgcagggt gatgccggtc
     1201 ttcgccagga ggatcccctc gacgtgcgcg ccgacgtgca gggtcgcctg gccggcgatc
     1261 tgccagaaga cgtttttggc ctgcgcgccg ccggcgagca gcacccgctt gctggtgctg
     1321 acgtcgaggt cgcccgagat ctggaagatc cagacgtcgt cctcgcagcc ctcgagggtg
     1381 acgtcgtccg tgatcaggac cgtgctgccc caggtgtaga tgccgggggc gagcgtcagg
     1441 ccgccgaggt cgccgcccgc gaggttgagg taatcggtgg ggacgcggcc ggccgcgtcg
     1501 gtgtaggcgg cctgcatggc gagcacggcg gtcgtcagca ggatcggcgt ggggaccgtg
     1561 taatcggccg cgtagacgcg aaacggcgcc ttgagcacgg gcgaggtcgc gtacacgccc
     1621 gggggcgcga gcacgagggc gaagccggtg atcgagctcg ccgcggccgg gctgatgccg
     1681 acgtgaccgc cgtcgatctt ggtgccgggc acgttggtga tcgcggcctt ggcgagcacg
     1741 gcgtaggcgc ccggcgcagc gaggtcgtcc gggccgccga gcacgaccgg cgcggggccg
     1801 agcttgccca tgcaggtggc gttctccgtg tcgccggtcg tgccggagga cgccgagtcg
     1861 ccggtgtcct ggggcccgcc ggtgcccgac tgcgaggagc cctggctgcc gccctgctcg
     1921 gtggccgcgg tggtgccctc atcggcgctc ccgtcggtgc ctccggtggt cgtcgtggcc
     1981 ccgccggtgg tcggcgcggc gtcgctgtcg tccgcctgcg aagtgccagt gctctgcgac
     2041 gcggcggtgt cgctgccgtc gaggtccatg gggtcggagc agccggcgag gaggagcagg
     2101 ctcaaataga tacgaggatt catgagacgc ctgtccggtc gccggcggtt cgctgtgaac
     2161 cggccttgaa cccacgagga tgtaatgtgt gcacggacgg cgaacgcctg tcccagccaa
     2221 catcacgacg tgctccttgt atcacctgca ggggccaatt cacaagggta cgcggcgaca
     2281 tggggcgttt tgggcgttgc cgcgggccgc ctcaaaacgc acgcgaagcg ccgggggccg
     2341 cgagctgaag ggatcgcggg gcgaggttcc tgtggccaat gtggatccgg cgattcggtg
     2401 cagttgctcg tgcgatcgtt tggtctcggt tgcgtcaccg gtggcagaca cgcggggtcc
     2461 gcggcgggag ccgtgcgcag gcggggaccc ctgccgcgat ctcgggcgcg ccggggtggt
     2521 tcggaccggt gcgccgcgcg accggcggga ggtggcccgg aggggcacgg acggggctgc
     2581 cggcggcgtc ctgtgtaatt tatgtttgcc agctcaccga agttgctgag gcgcgagagc
     2641 gagcggcgcc gctctccatg cgctacaacc acgcaccgtg aatgctccgc acacctcggc
     2701 cgacacgccg gtcatgcgtc agttcctcga catcaaggcc gggtatccgg acgcgatctt
     2761 gatgtttcgc atgggggact tctacgagat gttcttcgac gacgcgatca cggtcgcgcc
     2821 ggtgctcgac atcgcggtca cctcgcgcga caagggggac gtcgaccgcg tgccgatggc
     2881 aggggtgccg taccacgcga tcggcgggta cctgcggacg ctggtcgagc gcggcttcaa
     2941 ggtcgcgatc tgcgagcaga tggagacgcc cgagcaggcc cggctgcgca aggggccgaa
     3001 gatcgtgcgc cgcgaggtgg tccgggtcgt gacaccgggc gtgctcgtcg acgaggagca
     3061 cctgcgcagc gaggagccga actacctcct ggcgatcgtc gccgaggggg tcggcaccgg
     3121 cgcgcagtcg gagggcttcg gggtcgcggc gctcgatgtc agctgcggcg agttcttcgc
     3181 gctgcgctgc gtcgacgggg tgtcgctgcg ggccgcgctg tcgcgcctgg ctccgcgcga
     3241 gatcctcgtc gacgcggggc tgcacggatg gctgcgcgag gcgctcgggc cggcgtgtcc
     3301 gcggctggaa gggcgcgaca gcaaggcgct gcccgccgag atggtcgcgc gcgtgcagca
     3361 cctgcgcgcc gagagcggcg gggagccgct gctgcccagc gaggcgcggg cggccgcgct
     3421 gacgctcgcc tacgcggagg agacc
//
LOCUS       DASWNJ010001247         2334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_481547, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001247 DASWNJ010000000
VERSION     DASWNJ010001247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481547"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VGB85_20995"
     CDS             <1..405
                     /locus_tag="VGB85_20995"
                     /inference="COORDINATES: protein motif:HMM:NF016627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEY0136579.1"
                     /translation="GRVVTVVPEPGARCHGVAYRVAPEVAERVIADLDHRERGGYASV
                     TLDVELLGGPAPERARARMYLATPDNPHYLGPAPVDAIAGQILRSHGPSGPNAEYLLR
                     LADALRELEAEDDHVFTLERTLRALQAGLAAG"
     gene            469..1383
                     /locus_tag="VGB85_21000"
     CDS             469..1383
                     /locus_tag="VGB85_21000"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HEY0136580.1"
                     /translation="MSAVSLSLRLPVLGVLGAPWLAGCLLVSNPAYEDTGDATTGTPD
                     FDTTTGEPVFPCPDDQPFSLWYPDADADSYGDRDATPAMACEAPAGHVQDAGDCNDKS
                     PEIRPKLPEQCNGFDDNCNALVDESSPNCGACVIELTDSFVYWVCAQPGGITRAEARQ
                     HCVARSTKENPVRLASIHSAEEHARLAELVGLHVPPVGGEQHAWIGLVKREEAALSCD
                     PPDPVTAWRWDDGSDFNLDPPPWNWNEPNNSPQDCACDVAAGCVEDCGEIQVVPAQNL
                     SGWNDFRCDATSATGFICKTHRDPVLFP"
     gene            1468..1590
                     /locus_tag="VGB85_21005"
     CDS             1468..1590
                     /locus_tag="VGB85_21005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136581.1"
                     /translation="MTALKLALLVLLCLVLAPLALLGMALLPLLDLRPLTEDLG"
     gene            1587..2156
                     /locus_tag="VGB85_21010"
     CDS             1587..2156
                     /locus_tag="VGB85_21010"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEY0136582.1"
                     /translation="MTRKRLFDLALLLPLVPLFASVTGLLALAVLLADGRPVFFHQPR
                     VGAHLRPFTVWKLRTMTCEPDIAARKPTRLGQWLRQRGLDELPQLFNILRGDMSLVGP
                     RPLTPADATRLCALYPAFAARFAVAPGLTGLAQVCLAQGAATTARLDAAYVERRSAAL
                     DLKILVRTAWMNLVGKRRGAMPTPDNVTT"
BASE COUNT          308 a          985 c          723 g          318 t
ORIGIN      
        1 ggccgggtcg tcaccgtcgt gcccgagccc ggcgcccgct gccacggcgt cgcctaccgc
       61 gtcgcccccg aggtcgccga gcgcgtgatc gccgacctcg accaccgcga gcgcggcggt
      121 tatgccagcg tcaccctcga cgtcgagctg ctcggcggtc cggccccgga gcgtgcccgc
      181 gcgcgcatgt acctcgccac ccccgacaac ccccactacc tcggccccgc ccccgtcgac
      241 gcgatcgccg ggcagatcct gcgcagccac ggacccagcg gccccaacgc cgagtacctg
      301 ctgcgcctcg ccgacgccct gcgtgagctg gaggcggagg acgatcacgt atttacgctc
      361 gagcgcacgc tgcgggcgct ccaggccggt ctggccgccg gctgagcgac cgcggcgcgc
      421 gagaccgcaa acgcaggctc gccagcctgc gctaccctcg gagccgcgat gtccgccgtc
      481 tccctctcgc tccgcctccc cgtgctcggg gtcctcggcg cgccctggct cgccggctgt
      541 ctgctggtca gtaatcccgc atacgaggac accggcgacg cgaccaccgg cacgcccgac
      601 ttcgacacga cgaccgggga gcccgtgttc ccctgccctg acgaccagcc gttctcgctg
      661 tggtacccgg acgccgacgc cgactcctac ggcgaccgtg acgcgacccc ggcgatggcc
      721 tgcgaggccc cggccggcca cgtgcaggac gccggcgact gcaacgacaa gtcgccggag
      781 atccgtccca agctgcccga gcagtgcaac ggcttcgacg acaactgcaa cgcgctcgtc
      841 gacgagtcga gcccgaactg cggcgcctgc gtcatcgagc tcaccgacag cttcgtgtac
      901 tgggtctgcg cgcagccggg cggcatcacc cgcgccgagg cccgtcaaca ctgcgtcgcg
      961 cgcagcacca aggagaaccc ggtgcgcctc gccagcatcc actccgccga ggagcacgcc
     1021 cgcctcgccg agctggtcgg cctgcacgtc ccgcccgtcg gcggcgaaca gcacgcgtgg
     1081 atcggcctgg tcaagcgcga ggaggcggcc ctgagctgcg acccgcccga cccggtcacc
     1141 gcctggcggt gggacgacgg cagcgacttc aacctcgacc ccccgccgtg gaactggaac
     1201 gagcccaaca acagccccca ggactgcgcg tgcgacgtcg cggccggctg cgtcgaggac
     1261 tgcggcgaga tccaggtcgt gcccgcgcag aacctgtccg gctggaacga ctttcgctgc
     1321 gacgcgacct ccgcgaccgg cttcatctgc aagacgcacc gcgacccggt gctgttcccg
     1381 tgaaccccgc gaccgctcga cccgcgaacg cgaccgttca cccgacctcc gcctgtcatc
     1441 ccgccacgcg accgtcacct tgatcgggtg accgccctca agctcgcgct gctcgtgctc
     1501 ctgtgcctgg tcctcgcccc gctcgcgctg ctcggcatgg ccctgctgcc gctgctcgac
     1561 ctgcgcccgc tgacggagga cctgggatga cccgcaagcg actgttcgac ctcgccctcc
     1621 tgctgcccct cgtgccgctg ttcgccagcg tcaccggcct gctcgcgctc gccgtgctgc
     1681 tcgccgacgg ccgccccgtg ttcttccacc agccccgcgt cggcgcccac ctgcgaccct
     1741 tcacggtgtg gaagctgcgc acgatgacct gcgagcccga catcgccgcg cgtaaaccaa
     1801 cgcgcctcgg ccagtggctg cgccagcgcg gcctcgacga gctgccgcag ctgttcaaca
     1861 tcctccgcgg cgacatgagc ctggtcggcc cgcgcccgct gacgcccgcc gacgcgacgc
     1921 gcctgtgcgc cctgtatccg gccttcgccg cccgcttcgc cgtcgcgccc ggcctgaccg
     1981 gcctcgccca ggtctgcctc gcccagggcg ccgcgacgac cgcccgcctc gacgccgcct
     2041 acgtcgagcg ccgcagcgcc gccctcgacc tcaagatcct cgtgcgcacc gcctggatga
     2101 acctcgtcgg caagcgccgc ggcgcgatgc cgacgcccga caacgtcacc acatgaccct
     2161 tcgagcaggt catactggcc cgcccccgtg agcctgctgc gccacaccct gctcgccctg
     2221 accgccccgc gccgcctcgt gccgctcgtg gtcgtcagcg tgcccttggt cgccgcgcag
     2281 cacagcctct cgcgtgaccc gctggcgatg ccgctcggcg tgctcatgtg cgtc
//
LOCUS       DASWNJ010001248         2978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_658902, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001248 DASWNJ010000000
VERSION     DASWNJ010001248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658902"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /locus_tag="VGB85_21015"
     CDS             <1..1233
                     /locus_tag="VGB85_21015"
                     /inference="COORDINATES: protein motif:HMM:NF039296.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5924 family protein"
                     /protein_id="HEY0136583.1"
                     /translation="QLRPVTAPSPITSAPVTSGPITELTPEMAAAVADLERATDGDLP
                     PTPTSAAGPVLPEGITEQDLAALRRFARVRAFMARHGNKLWYLHSAYALTFGAFVVMY
                     ASKGYDNARWMVVLLGLGWLVLILFFRVFGTGPGQKEKIKDTKSRVRFYVMTLILKNL
                     YQSMLFFLVPFYYKTATFDDPNRFFVYALVLLAILSTVDVVFDQVLMRWKIPASTVYF
                     FILFACLNLVLPALLPDTRTLLTQMSAAAIAAVVFFTMHVPIRNLLRPAWVLALMTFV
                     AAAVFATYHGRHAIPPVPMYIATGAVGPSLLADGRLSMQVSKLHESLIQEMHALTDVV
                     IPGGHGNTLHHVWRREGVALQRGPVTPSGEPPPGTVRLRSTLRSFNLPSDLAGLWSID
                     VVTDDDQLVGRVPFEVIR"
     gene            1230..2564
                     /gene="aroA"
                     /locus_tag="VGB85_21020"
     CDS             1230..2564
                     /gene="aroA"
                     /locus_tag="VGB85_21020"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEY0136584.1"
                     /translation="MNATKPSPSICVRPPPGPVFATLSPPGSKSLTNRALILASLAAG
                     RSTLRGCLDSEDTRVMLAAIARFGLPHAICNEGTRVTVEGGGGPPRHLSDPEQPLEVA
                     TAGTAARFLTAVLAASPLRALVDGSPRMRERPMEGLLRALREQGAEIRCLAADDFLPI
                     EIVGARLHGGEIRLPRPPSSQFISALILAAALADAPTRIILEQGTPARPYVDMTLVAL
                     REFGGDARWEGLDTIVITPAPLLAREYAVEPDASAASYLLAIPAIFGGRVTIPDLGHA
                     SVQGDALFCDVLRRMGAEVTQDATSTTVAGSGPLLGANLDLSDMPDMTLTAAVLALHA
                     RGRSEIHGVEVLRHHESDRLAAATTELRKLGATVEELPGGLIIDPPANPRRDVAIDTY
                     LDHRMAMAFSLAGHVEIRDPACVSKTFPRYFEVLAELGMAGTAEMNDTVVDP"
     gene            2689..>2978
                     /locus_tag="VGB85_21025"
     CDS             2689..>2978
                     /locus_tag="VGB85_21025"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEY0136585.1"
                     /translation="MKSGDALELDLDALGPAGEGVGHHGDRAVHVPGGFPGERVDVRI
                     TNVSRQRPLAHAELRKVVTPHPGRRASPCVHHDTGEPGKRKQVLCTGCPLLSL"
BASE COUNT          404 a         1232 c          887 g          455 t
ORIGIN      
        1 cagctgaggc cagtcaccgc accgagcccg atcacctcag ccccggtcac ctcgggcccg
       61 atcaccgagc tcacccccga gatggccgcc gcggtcgccg acctcgagcg cgccactgac
      121 ggcgacctcc cgccgacgcc caccagcgct gcgggtcctg tcttgcccga gggcatcacc
      181 gagcaggacc tcgccgcgct gcgtcgcttc gccagggtgc gggccttcat ggcgcgtcac
      241 ggcaacaagc tgtggtacct gcactccgcc tacgcgctga cctttggcgc cttcgtggtg
      301 atgtacgcca gcaagggcta cgacaacgcc cgctggatgg tcgtgctgct cggcctgggc
      361 tggctggtgc tgatcctgtt cttccgggtg ttcggcactg gtccggggca aaaggagaag
      421 atcaaggaca ccaagtccag ggtccgcttc tatgtcatga ccctgatcct gaagaacctg
      481 tatcagagca tgctgttctt cctggtgccc ttttattaca agacggccac cttcgacgac
      541 cccaaccgct tcttcgtcta cgcgctcgtg ctcctggcga tcctctcgac cgtcgacgtc
      601 gtgttcgacc aggtcctgat gcgctggaag atcccggcct cgaccgtcta cttcttcatc
      661 ctgttcgcct gcctcaacct cgtgctgccg gcgctgctgc ccgacacgcg cacgctgctg
      721 acacagatgt ccgccgcggc gatcgccgcc gtcgtgttct tcacgatgca cgtcccgatc
      781 cgcaacctgc tgcggcccgc gtgggtgctc gcgctgatga ccttcgtcgc cgccgccgtg
      841 ttcgccacct accacggccg ccacgcgatc ccgccggtcc cgatgtacat tgccaccggc
      901 gccgtcggcc ccagcctgct cgccgacggc cgcctgagca tgcaggtcag caagctccac
      961 gagtcgctga tccaggagat gcacgccctc accgacgtcg tcatccccgg cggccacggc
     1021 aacaccctgc atcacgtgtg gcgccgcgag ggcgtcgcct tgcagcgggg ccccgtcacg
     1081 ccctccggcg agccgccgcc cggcaccgtt cgtctgcgct ccacccttcg ctcgttcaac
     1141 ctgccctccg atctcgccgg tctgtggagc atcgatgtcg tcaccgacga cgaccagctg
     1201 gtcggtcgtg tccccttcga ggtcatccga tgaacgccac caagccgtcc ccgagcatct
     1261 gcgtacgtcc ccccccgggc cccgtgttcg ccacgctctc gcccccgggc tcgaagagtc
     1321 tgaccaaccg cgcgctgatc ctcgcgtcgc tcgccgccgg ccggtcgacc ctgcgcggct
     1381 gcctcgactc cgaggacacc cgcgtcatgc tcgccgcgat cgcccgcttc ggcctgccgc
     1441 acgcgatctg caacgagggc acgcgtgtca ccgtcgaggg cggcggtggt cccccgcgtc
     1501 acctctcgga tcccgagcag ccgctcgagg tcgccaccgc cggcaccgcc gcccgcttcc
     1561 tcaccgccgt gctcgccgcc tccccgctgc gcgcgctggt cgacggcagc ccgcgcatgc
     1621 gcgaacgccc catggagggc ctgctgcgcg cgctccgcga gcagggcgcc gagatccgct
     1681 gcctcgccgc cgacgacttc ctcccgatcg agatcgtcgg cgcccgcctg cacggcggtg
     1741 agatccgcct gccgcgcccg ccgtcctcgc aattcatctc ggcgctgatc ctcgccgcgg
     1801 ctctcgcaga cgccccgacc cgcatcatcc tcgagcaagg cacccccgcg cgcccctacg
     1861 tcgacatgac cctcgtcgcc ctgcgcgagt tcggcggcga cgcccgctgg gagggcctcg
     1921 acacgatcgt catcaccccc gcgccgttgc tcgcccgcga gtacgcggtc gagcccgacg
     1981 cctccgccgc cagctatctc ctggcgatcc ccgcgatctt cggcggccgc gtcacgatcc
     2041 ccgacctcgg gcacgccagc gtccagggcg acgccctgtt ctgcgacgtg ctgcgccgca
     2101 tgggcgccga ggtcacgcag gacgcgacga gcaccaccgt cgccggcagc ggcccgctgc
     2161 tcggcgccaa cctcgacctc agcgacatgc ccgacatgac gctgaccgcc gccgtgctcg
     2221 ccctgcacgc ccgcggccgc agcgagatcc acggcgtcga ggtcctgcgt caccacgaga
     2281 gcgaccgcct cgccgccgcc actacagaac ttcgcaagct cggcgccacc gtcgaggagc
     2341 tgcccggcgg cctgatcatc gacccacccg cgaacccccg ccgcgacgtc gcgatcgaca
     2401 cctacctcga ccaccgcatg gcgatggcct tcagcctcgc cggccacgtc gagatccgcg
     2461 accccgcctg cgtcagcaag accttcccgc gctacttcga ggtcctcgcc gagctcggca
     2521 tggccggcac cgccgagatg aacgacacgg tcgtcgaccc ctgacctgtc cgttcggacc
     2581 tgtcccgcag gccatgcggg tggaacagca ggtggaacag cgggtcgagc agcagcggtg
     2641 ccccccgcgc cgcttggcgc ccccgccggt ctgtggcaca ttgcgcgcgt gaagtccggt
     2701 gacgccctcg agctcgacct cgacgccctc ggccccgccg gcgagggcgt cggccaccac
     2761 ggcgaccgcg ccgtccacgt gcccggcggc ttccccggcg agcgcgtcga cgtgcgcatc
     2821 acaaacgtct cgcgccagcg cccgctcgcc cacgccgagc tgcgcaaagt ggtcacgccg
     2881 caccccggcc gacgcgcctc cccctgcgtg caccacgaca ccggcgagcc cggcaagcgc
     2941 aagcaggtcc tgtgcaccgg ctgcccgctg ctctcgct
//
LOCUS       DASWNJ010001249         3631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_162173, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001249 DASWNJ010000000
VERSION     DASWNJ010001249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162173"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VGB85_21030"
     CDS             complement(<1..713)
                     /locus_tag="VGB85_21030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136586.1"
                     /translation="MTTPLTSVAPPTPLSRLAAAEDLGHICLVATDVDGTLTRAGKLD
                     PAALAAIWRLMVAGVTVVPVSGRPAGEVQGLVRYMPGVVRGLAENGLVEIVPDHAPRW
                     LGKPTDKQRLRAAGERLNDDFAARLRITGDDYCRLGDVAYERDGRDEAELLRLRGHAE
                     ALGVHLVWSNVHVHLAEAVPDKGVAVLQLLRELAIDPGRAATIGDAPNDAGLFIAGRF
                     GLTVGTADVTPQLAYFPSPP"
     gene            823..2031
                     /locus_tag="VGB85_21035"
     CDS             823..2031
                     /locus_tag="VGB85_21035"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing serine/threonine-protein
                     kinase"
                     /protein_id="HEY0136587.1"
                     /translation="MAVVLQPGDVYAGNIKVVSVLGAGAFANVYKVEVRGFDRPLALK
                     LTREPVTTSDAAQRALREITILRSLTNPHVVRTFDCGLRPDGHVYMLMDYLEGDTLNT
                     WHSFEEPLHPAQAVTIVHQACLGLAEPHAQGIVHRDVKPENIFVQDNGQIKVLDFGLA
                     RSWDGSPVIGANATQIHMVVGTPHYSQPEQLKTRVLTPASDVYSLATILYELLSAHTP
                     FHPDRPLNAVREEYREKPVRWLQAHANMPVFALDTMPGCEALPPALVDGIMLALDKDP
                     EKRPPDASAFANILGEVLHRDLGIPVAATLKLRWPEGGVEERLFLPGSYRIGSGERCE
                     IMLPDHHATHIRRVHAVLEWSGIPNRPYLRPIIGDGSVKIGDAPIRKPAELAPDDSFW
                     VGPYQLSLVM"
     gene            2101..3006
                     /locus_tag="VGB85_21040"
     CDS             2101..3006
                     /locus_tag="VGB85_21040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0136588.1"
                     /translation="MELKIQDLTKTYPNGVRALRGISLTIPAGMFGLLGPNGAGKSTL
                     MRTIATLQEPDTGSIRFGELDVLRQKSQLREVLGYLPQEFGLYPNLSPEAILEHFAVL
                     KGLHNRGERNAQVKALLEQTNLAAVRKKAVGGFSGGMKQRLGIAIALLGRPRLVIVDE
                     PTAGLDPTERHRFLNLLGEIGEDVVVILSTHIVEDVAELCPNFAIIAGGQLLYTGKPE
                     AVIATLRGKIWRKVTTRAELPALQKELRVISTRLITGRPVIQVYDEGNPGADFESVEP
                     SLEDVYFHHIASAAQPSVTPIARTA"
     gene            3003..>3631
                     /locus_tag="VGB85_21045"
     CDS             3003..>3631
                     /locus_tag="VGB85_21045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0136589.1"
                     /translation="MSQLLSFAAFELRHHLRRPITYVFMAAMFFLAFLFATTDAVSIG
                     GVGGKLVVNSPWVVNMAVMILTLIGMIMTSAISGTAVLRDFEIKAHELLFTTRLRKSS
                     FVFGRFLGAYAVTVLVFSCSALGLAAAMWWPWMDPERIGPGGLVNYLWPITVYILPNA
                     LLACALFFAVGIVSRSFTAVYVQGVVLFSGYSVALTLLSDLDNEAIAGLL"
BASE COUNT          569 a         1389 c         1113 g          560 t
ORIGIN      
        1 ggcggcgagg ggaagtacgc gagctggggc gtgacgtcgg cggtcccgac cgtcagcccg
       61 aagcgcccgg cgatgaacag gccggcgtcg ttgggcgcgt cgccgatggt cgccgcgcgc
      121 ccggggtcga tcgccagctc acgcagcagc tgcaacaccg cgacgccctt gtccggcacc
      181 gcctccgcca ggtgcacgtg aacgttcgac cagaccaggt gcacccccag cgcctccgcg
      241 tggccgcgca ggcgcagcag ctcggcctcg tcgcgaccgt cgcgctcgta cgcgacgtcg
      301 ccgagccggc aataatcgtc ccccgtgatc cgcagccggg ccgcaaaatc atcgttcaag
      361 cgctcgcccg cggcgcgcag gcgctgcttg tcggtcggct tcccgagcca gcgcggcgcg
      421 tgatccggca cgatctccac gaggccgttc tcggccagcc cacggaccac gcccggcatg
      481 tagcgcacga gcccctgcac ctcgcccgcg ggtcgccccg acaccggcac caccgtgacg
      541 cccgccacca tcagccgcca gatcgccgcg agcgccgcag gatcgagctt cccggcgcgc
      601 gtcagggtcc cgtcgacgtc cgtcgctacc agacaaatgt gccctaggtc ctccgccgcc
      661 gcgaggcgcg aaaggggcgt cggaggggca acggaggtga gtggcgtggt catcagggcg
      721 tcgtccggga gtccgcgggg ccgcggggcc gcgccgacaa ttcgtgcgcg gccttgctcg
      781 cgacctgcgg cctcgtgata gcttgtctcg agatccaaag gcatggccgt tgtacttcag
      841 cctggagacg tttacgcggg caacatcaag gtggtcagcg tcctcggggc tggcgccttc
      901 gcaaacgtct acaaggtgga ggttcgcggc ttcgatcggc cgctcgcgct caagctcacg
      961 cgcgagccgg tgaccaccag cgacgccgcc cagcgcgccc tgcgagagat cacgatcctg
     1021 cggtcgctga ccaacccgca cgtcgtccgc accttcgact gtggcctgcg ccccgacggc
     1081 cacgtctaca tgttgatgga ctacctcgag ggcgacaccc tcaacacctg gcactccttc
     1141 gaggagccgc tacaccccgc ccaggccgtc acgatcgtcc atcaggcctg cctcggcctc
     1201 gccgagccgc acgcgcaggg catcgtccac cgcgacgtaa aaccggaaaa catctttgtc
     1261 caggacaacg gccagatcaa agtcctcgac ttcggcctcg cccgctcgtg ggacggctcg
     1321 ccggtgatcg gcgccaacgc gacccagatc cacatggtcg tcggcacgcc gcactacagc
     1381 cagcccgaac agctcaagac ccgcgtgctc acccccgcga gcgacgtcta ctcgctcgca
     1441 accatcttgt atgagctgct ttcggcgcac acccccttcc accccgaccg cccgctcaac
     1501 gcggtgcgcg aggagtaccg cgagaagccg gtccgctggc tgcaagccca cgccaacatg
     1561 ccggtgttcg ccctcgacac gatgcccggc tgtgaggccc tgccgcccgc gctcgtcgac
     1621 ggcatcatgc tcgccctcga caaggacccc gagaagcgcc cgcccgacgc cagcgccttc
     1681 gctaacatcc tcggcgaggt tctccatcgc gacctcggca tccccgtcgc cgcgaccctc
     1741 aagctgcgct ggcccgaagg cggcgtcgag gagcgcctgt tcctccccgg cagctaccgc
     1801 atcggcagcg gcgagcgctg cgagatcatg ctcccggacc accacgcgac ccacatccgc
     1861 cgcgtgcatg ccgtcctcga gtggagcggc atccccaacc gcccctacct gcggccgatc
     1921 atcggcgacg gctccgtgaa gatcggcgac gcaccgatcc gcaaacccgc cgagctcgcc
     1981 cccgacgaca gcttctgggt cgggccctac cagctctccc tcgtgatgta gtcgtactcc
     2041 gagcttctga caccacgagg cctgattccg ggtctatgct cgcggacctc aggccgcgcc
     2101 atggaactca agattcagga cctcaccaag acctatccca acggcgtacg cgccctgcgg
     2161 gggatcagcc tcacgatccc ggccggcatg ttcggtctgc tgggccccaa cggcgccggc
     2221 aagtcgacct tgatgcgcac gatcgccacc ctgcaggagc ccgacaccgg cagcattcgc
     2281 ttcggcgagc tcgacgtgct gaggcaaaag tcgcagctgc gcgaggtcct cggttatctc
     2341 ccgcaggagt tcgggctcta cccgaacctc tcgcccgagg cgatcctcga gcacttcgcg
     2401 gtgctcaagg gcctgcataa ccgcggcgag cgcaacgcgc aggtcaaggc gctgctcgag
     2461 cagaccaacc tcgccgccgt gcgcaagaag gccgtcggcg ggttctctgg cggcatgaag
     2521 cagcgcctcg gcattgcgat cgcgctgctc ggccgcccgc gcctggtcat cgtcgacgag
     2581 ccgaccgccg gcctcgaccc gaccgagcgc caccgcttcc tcaacctgct cggcgagatc
     2641 ggcgaggacg tggtcgtcat cctcagcacg cacatcgtcg aggacgtcgc cgagctgtgc
     2701 cccaactttg cgatcatcgc cggcggccag ctgctctaca ccggcaagcc cgaggccgtg
     2761 atcgccaccc tgcgcggcaa gatctggcgc aaggtgacga cccgcgccga gcttccggcc
     2821 ttgcagaagg agctgcgcgt gatctccacg cgcctgatca ccgggcggcc ggtgatccag
     2881 gtctacgacg agggcaaccc gggcgccgac ttcgagtccg tcgagcccag cctcgaggac
     2941 gtgtacttcc accacatcgc cagcgccgcg cagcccagcg tcacgccgat cgcgaggacc
     3001 gcgtgagcca gctactgagc ttcgccgcgt tcgagctgcg gcatcacctg cggcgcccga
     3061 tcacctacgt gttcatggcc gcgatgttct tcctggcgtt cctgttcgcc acgaccgacg
     3121 cggtcagcat cggcggcgtc ggcggcaagc tggtcgtcaa ctcgccgtgg gtcgtcaaca
     3181 tggccgtcat gatcctgacc ctcatcggca tgatcatgac ctccgcgatc agcggcaccg
     3241 ccgtcctgcg cgacttcgag atcaaggccc acgagctgtt gttcaccacc cggctgcgca
     3301 agtcgagctt cgtattcggg cgcttcctcg gcgcctacgc cgtcaccgtg ctcgtgttct
     3361 cgtgcagcgc gctcggcctc gccgccgcga tgtggtggcc gtggatggac cccgagcgga
     3421 tcggccccgg cgggctcgtc aactacctct ggccgatcac cgtctacatc ctgcccaacg
     3481 cgctgctcgc ctgcgccctg ttcttcgccg tcggcatcgt cagccgcagc ttcaccgccg
     3541 tctatgtgca gggcgtcgtg ctgtttagcg gctactcggt cgccctcacc ctcctgtcgg
     3601 acctcgacaa cgaggcgatc gccggcctgc t
//
LOCUS       DASWNJ010001250         2790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_481562, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001250 DASWNJ010000000
VERSION     DASWNJ010001250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481562"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="VGB85_21050"
     CDS             <1..836
                     /locus_tag="VGB85_21050"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY0136590.1"
                     /translation="LLPHLKARAPGSTASTASTASSWRTQIEAGVAEWRETVRARAMQ
                     DANPLNPQRVVWELSARLPDRCIVTADSGTSAVWFARDLQLRRGMLASVSGTLATMGC
                     GVPYALAAKLAFPDRPVVALVGDGAMQMNGNSELITLARHWQAWKDPRLVVVVLNNRD
                     LNFVTWEMRLMGGSPRWPASQQVPDFPYARYAELLGLRGIRVDAPDQVGPAWDQALAS
                     DRPVVLEAFVDPDVPVLPPLFTRAQEKTLRGSLADDAEASGVRRQLERELEPGGVRRR
                     E"
     gene            1013..1855
                     /locus_tag="VGB85_21055"
     CDS             1013..1855
                     /locus_tag="VGB85_21055"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY0136591.1"
                     /translation="MVANTTDLPSDFKDHEDWPDEINVHPIERMASISIGLVFAGFGL
                     LRRGLGGAGIGLLGGALIHRGLTGHCQLYSALRVSTAHNTRGPSASVPHGQGIRIKHS
                     LNVRETPETLYQFWRQLENLPSFMRHIESVTVVDATRSRWRAHGPADQPIEWDAEIIN
                     DVPNELIAWRSLPGTLVPNAGSVRFERLLDNHGTKVTVTLEYDPPGGLIGTAIAKWLG
                     ADPNVQVEEDLQRLKTVFEAGEVPRVGAQWRGRLAEFPDLETARQVLESEAAPPIDDG
                     QLKN"
     gene            complement(1915..>2790)
                     /locus_tag="VGB85_21060"
     CDS             complement(1915..>2790)
                     /locus_tag="VGB85_21060"
                     /inference="COORDINATES: protein motif:HMM:NF013246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005254 - chloride channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HEY0136592.1"
                     /translation="VLLLVFAVACGVAAVHQFLNYKPSVAPLPFSVVGVALSIFLAFR
                     NNAAYDRYWEGRKLWGGLVNASRSFGRQITTMAAAPEGETEALRRFQRTVVYRQIAYV
                     NALRSLLRGDKIEEQQAALLDPAEAAAVVGLENPPVGLMQAQGRGLMEAATRGWISDR
                     RHVAIDATLSELTALQGGCERIKRTPIPLAYRFFAQTFVRIFVVMLPFALVEQLGLFT
                     IVASLMVAFVFLVLDRIGDIIQDPFTTNFNGLPLAAICRTIEVDLRRQLGETDVPPLL
                     TAQRVPGGHNTSVLL"
BASE COUNT          416 a         1017 c          977 g          380 t
ORIGIN      
        1 cgctgttacc ccacctcaag gcccgcgcgc ccggctcgac cgcctcgacc gcctcgaccg
       61 cctcatcatg gcggacgcag atcgaggccg gggtcgccga gtggcgggag accgtgcgcg
      121 cgcgcgcgat gcaggacgcc aacccgctca atccgcagcg ggtcgtctgg gagctgtcgg
      181 cgcggttgcc cgatcggtgc atcgtcacgg cggactcggg cacctcggcg gtgtggttcg
      241 cgcgcgactt gcagctgcgt cgcggcatgc tggcctcggt ctcgggcacg ctggcgacga
      301 tgggctgcgg cgtgccctac gcgctggcgg cgaagctcgc gttccccgat cgtccggtcg
      361 tcgcgctggt cggcgacggc gccatgcaga tgaacggcaa cagcgagctg atcacgctgg
      421 cgcgccactg gcaggcctgg aaggacccgc ggctggtcgt cgtcgtgctc aacaaccgcg
      481 acctcaactt cgtcacgtgg gagatgcgcc tgatgggcgg cagcccgcgc tggccggcgt
      541 cgcagcaggt gcccgacttc ccctacgccc gctacgccga gctcctcggg ctgcgaggga
      601 tccgcgtcga cgcgccggat caggtcggtc cggcgtggga ccaggccctc gccagcgacc
      661 gccccgtcgt gctcgaggcc ttcgtcgacc ccgacgtgcc cgtgctgccg cccctgttca
      721 cgcgggccca ggagaagacc ctgcgcgggt ccctggcgga cgacgccgag gcgtccggcg
      781 tgcgccgcca gctcgagcgc gagctcgagc cgggcggcgt gcgccgccgc gagtgaccgg
      841 cgccgccagc gacctcgcac gatcgcgcgc ggctgccacg ggcccgtggc ggcgctgcgg
      901 cggctgatcg cgcccacggg ccccggcacc gcgatcgagg cgcttgcgtg gcgccgacac
      961 cgggaccaag aaccttggcc gacgccccgc gggggcgccc aaggagactg ccatggttgc
     1021 gaacaccacc gatcttccga gcgacttcaa ggaccacgag gactggcccg acgagatcaa
     1081 tgtccacccg atcgagcgca tggcgtcgat ctcgatcggc ctcgtgttcg ccggcttcgg
     1141 cctgctgcgt cgcggcctcg gcggcgccgg catcggcctc ctcggcggcg cgctgatcca
     1201 ccgcggcctg accggccact gccagctgta ctcggcgctg cgcgtcagca cggcccacaa
     1261 cacgcgcggc ccctcggcca gcgtgccgca cggtcagggc atccggatca agcactcgct
     1321 caacgtccgc gagacgcccg agaccctgta tcagttctgg cgccagctcg agaacctgcc
     1381 ctcgttcatg cgccacatcg agtcggtcac cgtcgtcgac gccacgcgct cgcgctggcg
     1441 cgcccacggc cccgccgatc agccgatcga gtgggacgcg gagatcatca acgacgtccc
     1501 caacgagctg atcgcctggc gctcgctgcc cggcacgctg gtccccaacg ccggctcggt
     1561 ccgcttcgag cgcctgctcg ataatcacgg caccaaggtg accgtcaccc tcgagtacga
     1621 cccgcccggc ggcctgatcg gcaccgcgat cgccaagtgg ctcggcgccg accccaacgt
     1681 ccaggtcgag gaggacctcc agcgcctcaa gaccgtgttc gaggcgggcg aggtgccccg
     1741 cgtgggcgcg cagtggcgcg gtcgcctcgc cgaattcccc gacctcgaga ccgcccgcca
     1801 ggtgctcgag agcgaggcgg cgccgccgat cgacgacggt cagctcaaga actgacccgc
     1861 tcgcctgttc agcgcctgca cgcatcgcgt gcgggcgctg attcgcgagc accgctacag
     1921 cagcacgctg gtgttatgcc cacccggcac ccgctgcgcg gtcagcagcg ggggcacgtc
     1981 ggtctcgccg agctgccgcc gcaggtcgac ctcgatcgtg cgacagatcg cagcgagcgg
     2041 gagcccgttg aaattggtgg tgaacgggtc ctggatgatg tcgccgatgc gatcgaggac
     2101 caggaagacg aaggcgacca tcagcgaggc gacgatcgtg aacaggccga gctgctcgac
     2161 gagggcaaac ggcagcatca cgacgaagat gcggacaaag gtctgggcga agaagcggta
     2221 ggcgagcggg atcggggtgc gcttgatgcg ttcgcagccg ccttgcaggg cggtcagctc
     2281 gctcagcgtc gcgtcgatcg cgacgtggcg gcggtcgctg atccagccgc gggtggccgc
     2341 ctccatgagg cccctgccct gcgcctgcat caggccgacg ggagggttct cgagcccgac
     2401 gaccgcggcg gcctcggccg ggtcgagcag cgcggcctgt tgctcctcga tcttgtcgcc
     2461 gcgcaggagc gaccgcaggg cgttgacgta ggcgatctgg cgatacacca cagtccgctg
     2521 gaagcggcgc agcgcctcgg tctcgccctc gggagcggcg gccatcgtcg tgatctggcg
     2581 accgaagctg cgcgaggcgt tgacgagccc gccccacaac ttcctgccct cccagtagcg
     2641 gtcgtaggcg gcgttgttgc gaaacgcgag gaagatgctc agcgcgacgc cgacgaccga
     2701 gaacggcagc ggcgcgacgc tcggcttgta gttcaggaac tggtgcaccg ccgcgacgcc
     2761 gcacgcgacg gcgaacacga gcagcagcac
//
LOCUS       DASWNJ010001251         2444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_127413, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001251 DASWNJ010000000
VERSION     DASWNJ010001251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127413"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /gene="lnt"
                     /locus_tag="VGB85_21065"
     CDS             <1..1334
                     /gene="lnt"
                     /locus_tag="VGB85_21065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEY0136593.1"
                     /translation="PAPRLFIHLLFAGFQGLQWAVPAALLIWLRRRTGRDVLLLAPLL
                     WVAGEALLPHVFPSYLALMWCYQPLWLQLAEIGGVTTIGLAQLSINAGLYVLLRDWFT
                     DRRINRAAAAVFLGWLIGTPLYGAIRMAQVDAVMAAAPRVKVGVVQGNFGIYEWSKGR
                     AKYGILQRLQEQSARLEAEGAQLLLWGETGYPFSHTLPRRAGHDLPEGHPRRVRRHFT
                     APLIFGVVTSDPKASPYAWNTAMVLHRDSRLGDTYDKVFPLYFGEYVPLVDPAWYLKM
                     VPAASYINPGDRPGVLDVDGMRFGPLICYEDILPRFARETAAGGVHAFVNLTNDSWFG
                     KSKEQYQHMGLAVLRTIENRRALLRSVNAGTSVYVDPNGRILHQTEVTDPDMSETGPL
                     PATGFVADVPLMPLDYLTPYTRTGELFNGLVVLLIAGIAIRGRRRRSSNDR"
     gene            complement(1331..1885)
                     /locus_tag="VGB85_21070"
     CDS             complement(1331..1885)
                     /locus_tag="VGB85_21070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136594.1"
                     /translation="MRLRLALLALTSLTPLLAPKVAHATQLARATSYETPPRKGFYAE
                     LALRPGLVVLRDGVVPMIRHHFMFGAGLTDRFKLGMNLHIGGYLGGQMAKKPVLGLDV
                     VATGYVWRGLYLRGGGGVVNRLPLALGSSETRPGYGGLVGIGYEWTIQKAAGIGLGAD
                     FDMRLISPREVRHSFIAGLHFHFH"
     gene            complement(1934..2302)
                     /locus_tag="VGB85_21075"
     CDS             complement(1934..2302)
                     /locus_tag="VGB85_21075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEY0136595.1"
                     /translation="MTAVFHAYPGCSTCRSARRWLDEHGVAYREIDISVTPPSAGDLR
                     NIRNMAKVPVRRLFNTSGQVYRAGNYAARLADMDDDAAFAELAANGMLIRRPLLVLGE
                     TAVVGFRAEEYAEALAASNA"
BASE COUNT          366 a          872 c          826 g          380 t
ORIGIN      
        1 ggcccgcgcc gcggctgttc atccacctgc tgttcgccgg cttccagggc ctgcagtggg
       61 ccgtgcccgc ggcgctgttg atttggctgc gccgccgcac cggccgcgac gtgctgctgc
      121 tcgccccgct gctgtgggtc gccggcgagg ccctgctgcc gcacgtgttc ccctcgtacc
      181 tcgcgctgat gtggtgctat cagccgctgt ggttgcagct cgccgagatc ggcggcgtca
      241 ccacgatcgg cctcgcccag ctgagcatca acgccggcct gtatgtactg ctgcgcgact
      301 ggttcaccga ccgacggatc aaccgcgccg cggcggccgt gttcctcggg tggctgatcg
      361 gcaccccgct gtacggcgcg atccgcatgg cccaggtcga cgcggtgatg gccgcggcgc
      421 cgagggtcaa ggtcggcgtc gtccagggca acttcggcat ctacgagtgg tccaagggcc
      481 gcgccaagta cggcatcttg caacgcttgc aggagcagtc ggcccgcctc gaggccgagg
      541 gcgcgcagct actgctgtgg ggcgagaccg gctatccctt ctcgcacacg ctgccgcgtc
      601 gcgccggcca tgacctgccc gaggggcatc cgcgccgcgt gcgacgccac ttcaccgcgc
      661 cgctgatctt cggcgtcgtc acctcggacc cgaaggccag cccctacgcg tggaacaccg
      721 cgatggtgct gcatcgtgac agtcgcctcg gcgacaccta cgacaaggtg ttcccgctgt
      781 acttcggcga gtacgtgccg ctcgtcgacc cggcctggta cctcaagatg gtgcccgccg
      841 cctcgtacat caaccccggc gaccgccccg gcgtgctcga cgtcgacggc atgcgcttcg
      901 gcccgctgat ctgctacgag gacatcctgc cgcgcttcgc ccgcgagacc gcggccgggg
      961 gcgtccacgc cttcgtcaac ctgaccaacg actcgtggtt cggcaagtcg aaggagcagt
     1021 accagcacat gggcctggcg gtcctgcgca cgatcgagaa ccgccgcgcg ctgctgcgat
     1081 ccgtcaacgc cggcaccagc gtctacgtcg accccaacgg ccgcatcctg catcagaccg
     1141 aggtcaccga ccccgacatg tccgagactg gaccgctgcc cgcgaccggc ttcgtcgccg
     1201 acgtgccgct gatgcccctc gactatctca ccccgtacac gcgcaccggc gagctgttca
     1261 acggcctcgt ggtgctgctg atcgcgggca tcgcgatccg cggccgccgt cgtcgctcat
     1321 caaacgatcg ctagtggaaa tgaaagtgca gcccggcgat gaagctgtgg cgcacctcgc
     1381 gaggcgagat caggcgcatg tcgaagtccg cgccgaggcc gatgccggcg gccttctgga
     1441 tcgtccactc gtagccgatg ccgaccaggc cgccgtagcc cggtcgcgtc tcgctgctgc
     1501 cgagcgccag cggcaggcga ttgacgaccc cgccgccgcc gcgaaggtac agcccgcgcc
     1561 acacataacc ggtggcgacg acgtcgaggc cgagcaccgg ctttttggcc atctgccccc
     1621 cgaggtagcc gccgatgtgc aggttcatgc cgagcttgaa gcggtcggtc aggccggcgc
     1681 cgaacatgaa gtgatgccgg atcatcggga ccacgccgtc gcgcagcacg acgaggccgg
     1741 ggcgcagcgc gagctcggcg tagaagccct tgcgcggcgg cgtctcgtac gaggtggcgc
     1801 gcgcgagctg ggtcgcgtgg gcgaccttcg gggcgagcag cggagtcagc gaggtcaggg
     1861 cgagcagggc gaggcgtaag cgcatggcaa ggcagggaac gccgcgcgtg cgccatgcct
     1921 tacatcgccg ccgtcacgcg ttgctggcgg cgagcgcctc cgcgtactcc tcggcccgaa
     1981 acccgacgac cgcggtctcg ccgagcacga gcagcggacg acggatcaac atgccgttcg
     2041 ccgcgagctc ggcgaacgcg gcgtcgtcgt ccatgtctgc cagccgcgcc gcgtagtttc
     2101 ctgcgcggta gacctggccg ctggtgttga acagccgtcg caccggcacc ttcgccatgt
     2161 tgcggatgtt gcgcagatca ccggcgctcg gcggtgtgac cgagatgtcg atttcgcggt
     2221 acgcgacgcc gtgctcgtcg agccagcggc gcgcgcttcg gcaggtgctg caaccgggat
     2281 atgcgtggaa aacggccgtc ataccgggcg gaatcgtacc aggcccgcgc tgcgggagtc
     2341 tgcacaaaag gtcaggtcgc ctcgcggcgc cagcaggaat ggacggggcc ggaaaatttg
     2401 ccggagccta cctgtccgaa cggccagctc gcgtaacgtc gtcc
//
LOCUS       DASWNJ010001252         2655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_199816, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001252 DASWNJ010000000
VERSION     DASWNJ010001252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199816"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VGB85_21080"
     CDS             <1..475
                     /locus_tag="VGB85_21080"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEY0136596.1"
                     /translation="RARVPEPSPLAVALWELVRRHVGDDTIRLMQVREVWPRIAGAGL
                     EAHTWPATVAGDTLVVHVHDSQWLHELTYMRQDLLRKLATLAPLSRLRAIKLRLGEVP
                     PVAGRVLPETPRPAPLPYPRLSAEPDAATREALDAVTDPELKQIIAGARVMLGRS"
     gene            958..1989
                     /locus_tag="VGB85_21085"
     CDS             958..1989
                     /locus_tag="VGB85_21085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136597.1"
                     /translation="MILRDLKTIALVGSLAMLSACTINGGGDDGNDDTGTATDNPSTG
                     NPTTTPTTSGADTGGEDAQLCANFGGYAGVKTVVGDFVGRVLVDDRINAYFLSTDVDG
                     GKLTTCLEEQVGNATGCAGVTYTCGDMKTVHAGMGISMADFGDLAEDFSKAMDAHQVN
                     TPTLTQADKDAVLGVLGGMAGDIVEDANNNVTTYQKLGRKPGIATVIGGPSDPKSFVA
                     MVAGDATLVTFFAMSDLDRLKTCLVRQVTTATSGPQIYGKEVTAPPPADPGVTTENPC
                     KDMLSSHKDLKDSMGVGIEKADFDALVGHLVTAMNNYTVPMAEQNTIIGALGPMCKEI
                     VTVDPEMCG"
     gene            2068..>2655
                     /locus_tag="VGB85_21090"
     CDS             2068..>2655
                     /locus_tag="VGB85_21090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136598.1"
                     /translation="MALQPLRSIVTLVTLATLAAFACNGPSPAPAETPAPAAPADSAA
                     PDADSAAAKGLRGLASLVGGSKPIQPWTFDFKDPPAGPRAPATAAFGATIGTSMYPEV
                     EAYVQKLGLHCADTSVRAMMDRRRESERAKVEDARARGEDAVTAASWVNKRSKREANP
                     QVRFSCPKTTADQLTDRTRPPSSGRLLYIFDDTAYP"
BASE COUNT          430 a         1047 c          819 g          359 t
ORIGIN      
        1 ccgcgcccgc gtccccgaac cctcgccgct cgccgtggcg ctgtgggagc tcgtgcggcg
       61 ccacgtcggc gacgacacga tccggctcat gcaggtccgc gaggtgtggc cgcggatcgc
      121 cggggcgggg ctcgaggctc acacctggcc ggcgaccgtc gcgggcgaca ccctggtcgt
      181 gcacgtgcat gacagccagt ggctacacga actgacgtac atgcgccagg acctgctgcg
      241 caagctcgcc acgctcgcgc ccctgtcgcg gctgcgagcg atcaaactcc ggctcggcga
      301 ggtcccgccg gtcgccggcc gtgtgctgcc cgagaccccg aggccggccc ccctccccta
      361 tcctcggctg tccgccgagc ccgacgccgc gacccgcgag gcgctcgacg ccgtcaccga
      421 ccccgagctc aagcagatca tcgccggcgc gcgggtcatg ctcgggcgca gctagacgcc
      481 gatcactggc gccgcagcgc tcgcggcgag cgaccgatcc gcccgcgagc ctcgtgcgta
      541 ccgcagcgcc tccgcgcacg ccatgctcgt cgctcctgcg aagtatctgc gagatacgcc
      601 gtgcactcgc ttgcacggcc agtgcatgac cgtcgctgta cgcggaccgt ggcggcttcg
      661 cgtcagcgca cgagagcgct cgtcgatcgc ttccgatcct cgcataacga tggtgcatgc
      721 cggacgtgtc gagcacgtcg agcgtgccca ccaactcgac gttgaagttg tgacacacgg
      781 ctcgcacaac cgcccgcgga cccgtgtcac actccggccc tcgctcccgc acttcgttcg
      841 cgagctgtgc ttagaacccg gccccagcaa tgccgcccct gtcccgcttc cggggccggg
      901 acggcacgga ccttcgcagg ccccgtgggc ctgataacca ggagaagttg aatactcatg
      961 atccttcgtg acctcaaaac catcgccctc gtcggctccc tcgccatgct ctccgcctgc
     1021 accatcaacg gcggcggcga cgatggcaac gacgacaccg gcactgccac cgacaacccg
     1081 tccaccggta atccgaccac cacgccgacg acctccggcg ccgacaccgg cggcgaggat
     1141 gcccagctgt gcgccaactt cggcggctac gccggcgtca agaccgtcgt cggtgacttc
     1201 gtcggccgcg tgctcgtcga cgaccgcatc aacgcctact tcctgtcgac cgacgtcgac
     1261 ggcggcaagc tgacgacctg cctcgaggag caggtcggca acgcgaccgg ctgcgccggc
     1321 gtcacctaca cctgtggcga catgaagacc gtgcacgccg gcatgggcat ctccatggcg
     1381 gacttcggcg acctcgccga ggacttcagc aaggcgatgg acgctcacca ggtcaacacg
     1441 ccgaccctga cccaggccga caaggacgcc gtgctcggcg tgctcggcgg catggccggc
     1501 gacatcgtcg aggacgccaa caacaacgtg acgacctacc agaagctcgg ccgcaagccc
     1561 ggcatcgcga ccgtcatcgg cggcccgagc gaccccaaga gcttcgtcgc gatggtcgcc
     1621 ggcgacgcca cgctcgtcac gttcttcgcc atgtcggacc tcgaccgcct caagacctgc
     1681 ctcgttcgtc aggtcaccac ggcgacctcg ggcccgcaga tctacggcaa ggaggtcacc
     1741 gcccccccgc cggccgatcc gggcgtcacc accgagaacc cgtgcaagga catgctcagc
     1801 tcccacaagg acctgaagga cagcatgggc gtcggcatcg agaaggccga cttcgacgcg
     1861 ctcgtaggtc acctcgtcac cgcgatgaat aactacacgg tcccgatggc cgagcagaac
     1921 acgatcatcg gcgcgctcgg gccgatgtgc aaagagatcg tcaccgtcga tcccgagatg
     1981 tgcggctgat agccgtcccg cgcgccgtcg tcggccttgc cggcggcggc gcttcccgtc
     2041 ctcctctcct ttaggtagct tccccccgtg gccctgcaac cccttcgctc catcgtcacg
     2101 ctcgtcacgc tcgccacgct cgccgcgttc gcatgcaacg ggccgagccc cgcgcccgcc
     2161 gagacgcccg cgcccgcggc cccggccgac agcgccgcgc ccgatgccga cagcgccgcc
     2221 gcgaagggcc tgcgtggcct cgccagcctg gtcggcggga gcaagccgat tcagccgtgg
     2281 accttcgact tcaaggaccc gcccgcgggc ccgcgcgccc cggccaccgc cgccttcggc
     2341 gcgacgatcg gcacctcgat gtaccccgag gtcgaggcgt acgtgcaaaa gctcgggctc
     2401 cactgcgccg acacctcggt gcgcgccatg atggaccgcc gccgcgagag cgagcgcgcc
     2461 aaggtcgagg acgccagagc ccgcggcgag gacgccgtga ccgccgcgtc gtgggtcaac
     2521 aagcgcagca agcgggaggc caacccgcag gtccgcttca gctgccccaa gaccaccgcc
     2581 gaccagctga ccgaccgcac gcgcccgccc tcgagcggcc gcctgctcta catcttcgac
     2641 gacaccgcct acccg
//
LOCUS       DASWNJ010001253         2209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_405087, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001253 DASWNJ010000000
VERSION     DASWNJ010001253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405087"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..1637
                     /locus_tag="VGB85_21095"
     CDS             198..1637
                     /locus_tag="VGB85_21095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136599.1"
                     /translation="MLPRLTVLPFLMLTPLVLGLLMGSSAHAAPDDLAAAIPVTSPYH
                     APVVLGADAPPPLAAQPGVLFINFDGGEMNDCGWSNDPKNNCSWIFDGTVLPYSGDAG
                     QRAAVVQYIRKDFEAFAIKVTDIRPGDNVDYDMEMVGDWDPAPQGGFAGVAPSIDCFN
                     QTGGETSFTLDYTNSAGGIARAVLQEAAHTWGLEHVDSKADLLYPTTAGVQNPTFEDT
                     CHQIVVLNEANQVEPSQGECVSQHKQFCDSGDQQNSFRELLQIFGPPTPDLTAPELSI
                     LSPVQGAEVGTTFDLVLHAEDEQSPQLLAIEIVATGAADFTTDTTRASPSDLTYPIKG
                     LPAGDYTVTVTVTDEDDNLATAKVDFTVVATPSPTTAGEDDSGAADTGTTATDDTGAG
                     SSSGAGEDTGAGEDTGTPTTGDVPGDDSGPTPTTGPATGDASDSGDSGEGPMGEPSGD
                     DGCGCRSRPGGAITLLLPLALGLRRRRRS"
     gene            1678..>2209
                     /locus_tag="VGB85_21100"
     CDS             1678..>2209
                     /locus_tag="VGB85_21100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136600.1"
                     /translation="MRWFIVALALSSAPARADETPGRHLATAPVTQAAPPPLVLPQRA
                     QAPARPHLLYINFDGAVLRRGCGNDSRHDCSSLADRFNGYVGPFVGTEHRRLSIVQAV
                     RKDLADFGVRAILERPPAETGYTMVLYGDLGTQEFAGIAPYIDCDNLWENDTCFAGAF
                     QGSNIGSTIILQEAAHT"
BASE COUNT          347 a          872 c          711 g          279 t
ORIGIN      
        1 cgacgtcgac ctcacccgcg ccgcggccct cgactgcaag tacttcaacg cggccgtcgc
       61 ccgcggcgcc ttcgccctcc ccgagttcgc ccgaaggctg atcgatccgg cctgaccgcg
      121 caggccacgg gcaaggtcac acggacccac accgcgaccg gcgcgtggcc acggtcgcgc
      181 gtgatacgtt ggccgcaatg ctgccacgcc tcacagtcct gccgttcctg atgctgaccc
      241 cgctcgtttt gggtctcctg atgggcagtt cggcccatgc ggcaccggat gacctcgccg
      301 cggcgatccc cgtgacctcg ccatatcacg cgccggtggt gctcggggcc gacgctccgc
      361 cgccgctcgc ggcccagccc ggcgtgctgt tcatcaactt tgatggcggc gagatgaacg
      421 actgcgggtg gagcaacgac ccgaagaaca actgctcgtg gatcttcgac ggcacggtgc
      481 tgccctactc gggcgacgcc ggccagcgcg cggcggtcgt gcagtacatc cgcaaggact
      541 tcgaggcctt cgcgatcaag gtcaccgaca tccgcccggg cgacaacgtc gactacgaca
      601 tggagatggt cggcgactgg gacccggccc cgcagggcgg cttcgccggg gtcgcgccga
      661 gcatcgactg cttcaaccag accggcggcg agaccagctt caccctcgac tacaccaaca
      721 gcgccggcgg catcgccagg gccgtgctgc aggaggccgc gcacacctgg ggtctcgagc
      781 acgtcgactc caaggccgac ctgctgtacc cgaccaccgc cggcgtgcaa aacccgacct
      841 tcgaggacac ctgccaccag atcgtcgtgc tcaacgaagc caaccaggtc gagccgagcc
      901 agggcgagtg cgtcagccag cacaagcagt tctgtgactc gggcgaccag cagaacagct
      961 ttcgcgagct gctgcagatc ttcggcccgc cgacgcccga cctgaccgcg ccggagctga
     1021 gcatcctcag cccggtgcag ggcgccgagg tcgggacgac cttcgacctc gtgctccacg
     1081 ccgaggacga gcagtctccg cagctgctcg ccatcgagat cgtcgccacg ggcgcggccg
     1141 acttcaccac cgacacgacg cgcgcgagcc ccagcgacct cacctacccg atcaagggcc
     1201 tgcccgccgg cgactacacg gtcacggtca ccgtcaccga cgaggacgac aacctcgcca
     1261 ccgccaaggt cgacttcacc gtcgtcgcca caccgagccc gacgaccgcg ggcgaggacg
     1321 actccggcgc cgcggacacc ggcaccaccg cgaccgacga caccggcgcg ggctcgtcct
     1381 ccggcgcggg cgaggacacc ggcgcgggcg aggacaccgg cacgccgacc accggcgacg
     1441 tgcccggcga cgacagcggc ccgaccccga ccacgggccc ggccaccggc gacgccagcg
     1501 actcgggcga ctcgggcgag ggtccgatgg gcgagccgag cggcgacgac ggctgcggct
     1561 gccgcagccg cccgggcggc gcgatcacgc tgctgctccc gctcgcgctc ggcctgcgtc
     1621 gccgtcgtcg cagctgagcc ggtgcgtcgc gccgcgatcg gcggtagcct ggcggcgatg
     1681 cgctggttca tcgtggcact cgccctctcc tccgcgcccg cgcgcgccga cgagaccccc
     1741 gggcgccacc tcgccaccgc cccggtgacg caggccgccc cgccgccgct cgtgctgcca
     1801 caacgcgcgc aggcgcccgc tcgcccgcac ctgctgtaca tcaacttcga cggcgccgtg
     1861 ctgcgtcgcg gctgcggcaa cgactcgcgc cacgactgca gcagcctggc cgaccgcttc
     1921 aacggttacg tcgggccctt cgtcggcacc gagcaccgcc gcctctcgat cgtgcaggcg
     1981 gtgcgcaagg acctcgccga cttcggcgta cgcgcgatcc tcgagcgccc gcccgccgag
     2041 accggctaca cgatggtgct ctacggcgac ctcgggaccc aggagttcgc gggcatcgcc
     2101 ccctacatcg actgcgacaa cctgtgggag aacgacacct gcttcgcggg cgcgttccag
     2161 ggctcgaaca tcggctcgac gatcatcttg caggaggccg cgcacacct
//
LOCUS       DASWNJ010001254         2295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_23191, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001254 DASWNJ010000000
VERSION     DASWNJ010001254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23191"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VGB85_21105"
     CDS             <1..379
                     /locus_tag="VGB85_21105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136601.1"
                     /translation="VADVERVYQARMLGPRGHAELSHFEERLRSVLGPRMQPLAIELL
                     TEAAHGPLTGEAATILVDDYLPDADPRQERLPFLREVLAILEHDGYLRNDDLGYVFAS
                     RLVGDWWRARFGFGFVPAAKRRS"
     gene            382..>2295
                     /locus_tag="VGB85_21110"
     CDS             382..>2295
                     /locus_tag="VGB85_21110"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0136602.1"
                     /translation="MATLRLKHNPSHMPDDELIRSFVVRQRDLAAILETVRENTGPSN
                     QHLLILGPRGMGKTTLVNRAVAEIRRDNALNRLWYPILFDEESYTVTSAGELWFQALS
                     HLADQTQDREIDRARRSLQHITDRARLRDASLARLLEFADAQKKRLLLVFENIDMIFD
                     EQLTEDDAWDVRHTLQNEPRIAVLATSPTRLDSFHDSDKPLYELFREHALDRLDLEEC
                     RTVWQLITGSELPLNQARPIQIFTGGNTRLLTILATFAKDRSFRELMDDLVGLIDENT
                     PYFKHNVEALPKESRKIFVTLAELWSPATARQVADQTRGDIRAVSAQLGRLQQQGIIE
                     VAKRVDKTMMYQVAERMYNLYHLLRRRGSPRVRAVVEFIVRYYDQDGMQRLLASMATE
                     AVGLDNDRREDHIRAYCELLRRLPDQQQRHEVIGHAPAQFLEIPDVALLLSELKTDPL
                     DVPILEEPDLRMRLAESPADSVALHQLINILLSRGENDQALELLERSVLIDGGSLLAV
                     SLTIRVLFSAPTITPESRVDYLRRLAARAKDGALELALSALALGLAVIGHDEEARELA
                     NEALERWPNNPLTGFLVGGVFSRLGHRGMALDAFTHAVDLDASSVEAVLSLVRLLVDD
                     RKFQEAEDRLRSCL"
BASE COUNT          428 a          806 c          665 g          396 t
ORIGIN      
        1 tgtggccgac gtcgagcgcg tctaccaggc ccggatgctc ggcccccgcg gccacgccga
       61 gctcagccac ttcgaggagc gccttcgcag tgtcctcgga ccccgcatgc agccgctggc
      121 catcgagctg ctcacggagg ccgcacacgg cccgctgacc ggcgaggccg cgaccatcct
      181 cgtcgatgac tacctcccgg acgcggaccc ccggcaggag cgcttgccct tcctgcgcga
      241 ggtgctggcg atcctcgagc acgacggcta cctgcgcaac gacgacctgg ggtacgtatt
      301 cgcatcgcgc ctcgtcggcg actggtggag ggcacgcttc ggcttcggct tcgtccccgc
      361 cgcgaagcgg aggagctgac gatggccacg ctgcgcctca agcacaaccc ctcgcacatg
      421 ccggacgacg agctgatccg cagcttcgtc gtccgccagc gcgacctcgc tgccatcctc
      481 gagaccgtcc gcgagaacac cggcccctcc aaccagcacc tgctcatcct cggcccgcga
      541 gggatgggca agaccactct ggtcaaccgc gcagtcgccg agatccgccg cgacaacgcg
      601 ctaaatcggc tgtggtaccc gatcctcttc gacgaggaga gctacacggt caccagcgcc
      661 ggggaactgt ggttccaggc gctgtcgcac ctcgccgacc agacgcagga ccgcgagatc
      721 gatcgcgcaa ggcgttcact gcaacacatc accgaccgcg cccgcctccg tgacgcctcg
      781 ctcgcccgcc tgctcgagtt cgccgacgcc cagaagaagc gactgctgct ggtcttcgag
      841 aacatcgaca tgatcttcga cgagcagttg accgaggacg acgcctggga cgtgcgccac
      901 acgctgcaaa atgagcctcg catcgcggtc ctcgccacgt cgccgacccg cctcgactct
      961 ttccatgaca gcgacaagcc gctctacgag ctgttccgcg agcacgccct cgaccgcctc
     1021 gatctcgagg agtgccgcac cgtctggcaa ctgatcaccg gctcggagct gcccctgaac
     1081 caggcccgcc cgatccagat cttcacgggt ggaaacacgc gcctgttgac gatcctcgcc
     1141 accttcgcca aggaccgatc gttccgcgag ctgatggacg acctcgtcgg cctcatcgac
     1201 gaaaatacgc cctacttcaa gcacaacgtc gaggctctcc cgaaggagag ccgcaagatc
     1261 ttcgtcaccc tcgcggagct ctggagcccc gcgactgccc ggcaggtcgc ggaccagacg
     1321 agaggcgaca tccgggccgt cagcgcccaa ctcggccgat tgcaacaaca gggcatcatc
     1381 gaggtcgcca agcgcgtcga caagaccatg atgtatcagg tcgccgagcg catgtataac
     1441 ctttaccact tgctgcgccg ccgcggctca ccccgtgtgc gggccgtcgt agaattcatc
     1501 gtccgctact acgatcagga cggaatgcag cgtctcctgg cctcgatggc gaccgaggcc
     1561 gtcggcctcg acaacgatcg ccgcgaggat catattcgag cgtactgcga actcctgcga
     1621 cgtctgcccg accaacaaca gcgccacgaa gtcataggcc acgcgccggc acaatttctc
     1681 gagataccag acgtcgccct gttgctcagt gaactgaaaa ctgacccact ggatgttcct
     1741 atcctcgaag agccagatct acgcatgcgt ctggccgaat caccggccga cagtgtggca
     1801 ctgcaccagc tcatcaatat actgcttagc cgtggggaga acgaccaagc gctggagttg
     1861 ctggagcgct ctgtgctgat cgacggcgga tcactcctgg ccgtgtctct tacgatacga
     1921 gtgcttttta gcgctccgac cattacgccc gaatctcgcg tcgattacct acgccggctc
     1981 gccgccaggg caaaggacgg tgcactcgag cttgctctgt ctgctctcgc gctgggtctg
     2041 gcggtgattg gtcatgatga agaggcgaga gaactcgcta atgaagctct cgagagatgg
     2101 ccgaacaatc ccctgacagg cttcctggtc ggcggcgtat tttcacgcct tgggcatcgc
     2161 ggcatggctc tggatgcttt tactcatgcc gtcgatctcg acgcttcgag cgtcgaggca
     2221 gtcctctccc tggtcaggct actggtcgac gaccgtaagt tccaggaggc cgaggaccgt
     2281 ttgcgaagct gtctg
//
LOCUS       DASWNJ010001255         1605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_659118, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001255 DASWNJ010000000
VERSION     DASWNJ010001255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659118"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VGB85_21115"
     CDS             complement(<1..395)
                     /locus_tag="VGB85_21115"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY0136603.1"
                     /translation="MGRYVFKLPDVGEGTAEAELVGWHVKVGDMVAEDQIIADIMTDK
                     ATVELTSPVAGKVLAAHGEPGQQLAVGSPLVEFEVEGAGNAAGAEAPPPSNPAAQASV
                     PLTGGQTASVSSPRSGEGDREAVEGRRVSL"
     gene            complement(407..1213)
                     /locus_tag="VGB85_21120"
     CDS             complement(407..1213)
                     /locus_tag="VGB85_21120"
                     /inference="COORDINATES: protein motif:HMM:NF039197.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5694 domain-containing protein"
                     /protein_id="HEY0136604.1"
                     /translation="MGALALLGAAVNPALAAQETPQRQRAQVMILGTFHFHNPNSDAA
                     QFKGIDVMSEARQSEIVGVVADLARFQPTRIALEVRIGQGDHAQRLQDEYARYRAGEL
                     TLSSNETHQLGFRLASQFGHSAVYPVDYRLGLDIGELMTYATANDPAFATWFQDYIAD
                     ATRQIDAMQGAETINRNLRWMNEPETLALAQTNYARMASVGANDTYIGARVAAQWYQR
                     NLHIFGNLARIATPGERVLLIVGQGHAPILRQLVRDHPDMELVEPNDYLR"
     gene            complement(1249..>1605)
                     /locus_tag="VGB85_21125"
     CDS             complement(1249..>1605)
                     /locus_tag="VGB85_21125"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0136605.1"
                     /translation="TILCYGTMVWVSLAGAEHAGVDAEVIDLRTLVPLDIETIEASVK
                     KTGRCVIVHEAPKTSGFGGELAALVQERCFYHLEAPIARVTGWDTPYPHAFEWEYFPG
                     PQRVADALKWAMGSRP"
BASE COUNT          254 a          521 c          545 g          285 t
ORIGIN      
        1 agcgagactc ggcgcccctc caccgcttcg cggtccccct ccccactgcg tggggaggag
       61 acggaggccg tctgtccccc tgtcaggggg accgaagcct gcgcagcagg gttcgagggg
      121 ggcggcgcct cagcaccggc cgcgtttccc gcgccctcga cctcgaactc caccagcggc
      181 gaacccaccg ccagctgctg gcctggctcg ccgtgcgcgg cgagcacctt gcccgcgacc
      241 ggcgaggtca gttccaccgt cgccttgtcg gtcatgatgt cggcgatgat ctggtcctcg
      301 gcgaccatgt cgccgacctt gacgtgccag ccgaccagtt cggcctcggc ggtgccttcg
      361 cccacgtcgg ggagtttgaa gacgtagcgg cccatctcaa gcgcccctaa cggagataat
      421 cattcggctc tacgagctcc atatcagggt ggtcgcggac gagctgcctc aggatcggcg
      481 cgtggccctg cccgacgatc agcaacacgc gctctccggg cgtcgcaatg cgcgcgaggt
      541 ttccgaagat gtggagattg cgctgatacc actgcgccgc cacgcgcgcc ccgatgtagg
      601 tgtcgttggc gccgaccgag gccatcctgg cgtagttcgt ctgcgccagc gccagggttt
      661 cgggttcgtt catccagcgc aggttgcggt tgatcgtctc ggcgccctgc atggcgtcga
      721 tctgacgggt ggcgtcggcg atgtagtcct gaaaccaggt cgcgaacgcc ggatcgttgg
      781 cggtggcgta ggtcatcagc tcgccgatat cgagcccgag gcggtaatcc acgggataga
      841 ccgccgaatg gccgaactgc gaagcgagcc ggaaacccaa ctggtgggtc tcgttgctgc
      901 tgagggtcag ttcgccggcg cgatagcggg catactcgtc ctgcagccgt tgagcgtggt
      961 ccccctggcc gatgcggacc tcgagcgcga tccgggtcgg ctgaaagcgg gcaaggtccg
     1021 ccacgacgcc gacgatctcg ctctggcggg cttcggacat gacgtcgatg cccttgaact
     1081 gggccgcgtc ggagttgggg ttgtgaaagt gaaaggtgcc caggatcatc acctgtgcgc
     1141 gttgtcgctg aggcgtctcc tgcgcggcca aggcgggatt gacggctgcg cccagcaggg
     1201 ccagcgcgcc catcaaggct gatatcagtc gcgggtatac aaatcgcgtc atggccggct
     1261 ccccatcgcc catttcaacg cgtcggcgac gcgctgcgga ccgggaaagt actcccactc
     1321 gaaggcgtgc ggataggggg tgtcccagcc ggtgacgcgg gcaattgggg cctccaggtg
     1381 ataaaaacag cgctcttgta cgagtgctgc aagttcgccg ccgaagcctg acgttttggg
     1441 cgcctcatgg acgattacgc atctgccggt tttcttcacc gacgcctcga tggtctcgat
     1501 gtcgagcggc acgagggtgc gcaggtcgat gacctcggcg tcgacgccgg cgtgctccgc
     1561 gcccgccagc gagacccaga ccatggtgcc gtagcagagg atggt
//
LOCUS       DASWNJ010001256         2106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_374385, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001256 DASWNJ010000000
VERSION     DASWNJ010001256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374385"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..>2106
                     /locus_tag="VGB85_21130"
     CDS             164..>2106
                     /locus_tag="VGB85_21130"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEY0136606.1"
                     /translation="MPGIESCCPATISPGDRPGQLSDLVRERENMNTHTFTDELKHTI
                     AVRPQEVVVVVGAGVTIGALRGSPLAEQASWHGLLLGGLERLRATGASPAADVDRIRG
                     LLDSDDVNLWIDAAQMITRGLGGPRGGEQRAWLRASVGAFAGAVHDRDVLTSLRALSE
                     RGVLLATVNYDGVLEAATGLPAVTWRDAARVQRVLRGDERGILHLHGYWEEPESLVFG
                     ARSYDEVVADEHARTVLKSLGMQRTFLFVGHGTGLQDPNWGSFLRWAEAVLAGMEHRH
                     YRLVRDEERVRVQAEHPQGQRVVALPYGATHGALAPFLRSLLPAAPNAAPPGAAAPVA
                     TSPRPVRTIVLRINIGDPNDRGFHPVSEQEARDLVDVPDPTFHALEAQVEPRRLTARN
                     WRGLAQDVDDLLTRMRGGIGVKEAVRIVIVGQAPLPVFAYLGYQMRRERGQIELINYR
                     QRGDAWDHIGAPGVAVDGDDGPFQVDEPELGRERRGKVVLSILCSNEYPYNPSMVEPM
                     ITAEGAALLCSYRIQHKRKHDDAPMDHADLTVLVGLVNRALVWIKDRCPDSDGLVVAM
                     GGPTWAAFWVARALNANVRGRIDFPYFTPGRGYERALAARMHEAPWFTGRAKLMIMAA
                     EPDDQGSVRAGRAADAIQQALER"
BASE COUNT          327 a          724 c          748 g          307 t
ORIGIN      
        1 gctgatcagc ggcggacccg ttgaagcttg ccgtagcgaa cctcgacaag atcgacaggc
       61 tcgactcgcc gccgctgctc agcagcggac ccgttgaagc ggaatcgggt cgacctggag
      121 caaccgaacg cgtcgagcgc gtccgtggcc aggctcaacg agtttgccgg ggatcgaaag
      181 ctgctgtccc gcgactatat ctcccggcga ccgccctggg cagctgagcg acctcgtgcg
      241 agaacgggag aacatgaata cacacacatt tacggatgaa ctaaagcata cgatcgccgt
      301 gcggcctcag gaggtcgtgg tggtcgtggg cgctggcgtg accatcggcg cgctgcgggg
      361 cagcccgctg gcggagcagg cctcatggca cgggctgctg ctcggcgggc tcgagcgtct
      421 gcgtgcgacc ggcgccagcc cggccgcgga tgtcgaccgg atccgcggct tgctcgacag
      481 cgacgacgtg aacctctgga tcgacgcggc gcagatgatc acccgcggcc tgggcggacc
      541 gcgcggcggg gagcagcgcg catggctgcg ggcgagcgtg ggcgcgttcg cgggggcggt
      601 ccacgaccgc gacgtgctga cctcgctgcg ggcgctcagc gagcggggcg tgctgctcgc
      661 caccgtcaac tacgatggtg tgctcgaggc cgcgaccggc ctgccggcgg tcacctggcg
      721 cgacgccgcc agagtccagc gggtgctccg gggcgacgag cgcggcatcc tccatcttca
      781 cggatactgg gaggagcccg agtcgctggt gttcggcgcc cgctcctatg atgaggtcgt
      841 cgccgacgag cacgcccgga ccgtgctcaa gtcgctggga atgcagcgca ccttcctgtt
      901 cgtgggccac gggacgggcc tccaggaccc caattggggc agcttcttgc gctgggcgga
      961 ggccgtgctc gcgggcatgg agcaccgcca ctaccggctc gtgcgcgacg aggagcgcgt
     1021 ccgcgtccag gccgaacacc cgcaggggca gcgggtcgtg gcgctcccgt atggggcgac
     1081 ccacggcgcg ctcgcgccct tccttcgctc cctgctcccc gcggcgccga acgccgcccc
     1141 gcccggcgct gcagccccgg tcgcgacctc gccgcggccc gtacgcacga tcgtgctgcg
     1201 catcaacatc ggcgacccga acgaccgcgg cttccatcca gtgagcgagc aggaggcccg
     1261 cgacctcgtc gacgttcccg atcccacctt tcacgcgctc gaggcccagg tcgagcccag
     1321 gaggctcacc gctcgcaact ggcgggggct cgcgcaggac gtcgacgacc tgctcacgcg
     1381 gatgcgcggg ggcatcggcg tgaaggaagc cgtgcggatc gtcatcgttg gacaggcgcc
     1441 gctccccgta ttcgcctatt tggggtacca gatgcggcgc gagcgcggcc agatcgagct
     1501 catcaactat cgccagcgtg gcgatgcctg ggaccatatc ggcgcgcctg gggtcgccgt
     1561 ggacggcgac gacggcccgt ttcaggtcga cgagccggag ctcggccgtg aacggcgggg
     1621 caaggtggtg ctctcgatcc tgtgctcgaa cgagtatccc tacaacccga gcatggtcga
     1681 gccgatgatc acggccgagg gcgccgcctt gctgtgttcg tacaggatcc agcacaagcg
     1741 caagcacgac gacgcgccga tggaccacgc cgatctgact gtcctcgtcg gtctggtcaa
     1801 ccgagcgctg gtatggatca aggacaggtg tcccgactcg gatggcctag tcgtggcgat
     1861 gggcggtcca acctgggccg cgttctgggt cgccagggcg ctcaacgcca acgtacgcgg
     1921 ccgcatcgac ttcccctact tcacccccgg ccgcggctac gagcgcgccc tggccgcccg
     1981 gatgcacgag gcgccgtggt tcaccgggcg ggcgaagctg atgatcatgg cagccgagcc
     2041 cgacgatcag ggcagcgtgc gcgccggtag agccgcagac gcgatccagc aggcgcttga
     2101 gcgcga
//
LOCUS       DASWNJ010001257         1765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_23251, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001257 DASWNJ010000000
VERSION     DASWNJ010001257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23251"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VGB85_21135"
     CDS             complement(<1..253)
                     /locus_tag="VGB85_21135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136607.1"
                     /translation="MLIDHVSVSGGVDKNVDVDQASDITVQWSVIAEAATEGHPESEH
                     NNGMLNNEGRISVHHVLFAHNKNQNPALASGPAESINNTA"
     gene            complement(261..>1765)
                     /locus_tag="VGB85_21140"
     CDS             complement(261..>1765)
                     /locus_tag="VGB85_21140"
                     /inference="COORDINATES: protein motif:HMM:NF018449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HEY0136608.1"
                     /translation="TTGMPGDCTPGDTQPCYGGPPGTEGIGLCAAGEQLCTPQGEWAP
                     CLGEVLPAAESCVTPGDENCDGVDACGGTGTYQWDHAFGSSSRENGLRVAFDGAGNIV
                     LAAHSKGDVDLGGGLLAGAGSYDLLLARFAPDGAHLWSKRFGDAANQFDDGYALDVAA
                     TGEIVLSGDFEGKIDFGGGPLDAQSSGDLFLVKLAADGTHVWSKVFHAPNGYAVPGAV
                     TRDDTGSILLGGYFVSGLDLGGGMLTSAGLADAFVGKFDPDGKHLWSQRFGDPQGQYV
                     QGLATDLAGNVFMTGGFQGTTNFGNGPLVSAGDADIFLARLDPKGTAVWSKRFGNAQL
                     QTGRDLAIDAAGRLVITGELQGTVDLGGGPVTAMSTAGYVAQFDSAGAHQWSRLVGDG
                     KILSRAVAYDGLGQVLLTGNFNDTVDFGGGPLISEGGGDIFALKLDGAGEHVWSKRFG
                     DFQIQTGTDIAGSATGIVALTGQFVSGLNFGGGPKTSKGDYDIFLVVFGP"
BASE COUNT          229 a          589 c          665 g          282 t
ORIGIN      
        1 atgcggtgtt gttgatcgac tcggcgggcc cggacgcgag ggcggggttt tggttcttgt
       61 tgtgggcgaa cagcacgtgg tggacgctga tccggccctc gttgttgagc atcccgttgt
      121 tgtgctcact ctcggggtgg ccctcggtcg cggcctcggc gatcaccgac cactgcacgg
      181 tgatgtcgct ggcctggtcg acgtcgacgt tcttgtcgac gccgccggag accgacacat
      241 gatcgatgag cacctgcagg tcacgggccg aagacgacga ggaagatgtc gtagtcgccc
      301 ttgctggtct tcgggccgcc gccgaaattg agcccgctga cgaactgacc ggtcagcgcg
      361 acgatgccgg tcgccgagcc ggcgatgtcg gtgccggtct ggatctggaa gtcgccgaag
      421 cgcttgctcc acacgtgctc gccggcgccg tcgagcttga gcgcgaagat gtcgccgccg
      481 ccctcggaga tcagcggacc gccgccgaag tcgacggtgt cgttgaagtt gccggtcagc
      541 agcacctggc cgaggccgtc gtacgcgacc gcgcgcgaca ggatcttgcc gtcgccgacg
      601 aggcggctcc actggtgcgc gcccgcgctg tcgaactggg cgacgtagcc ggccgtcgac
      661 atggccgtga cggggccgcc gccgaggtcg acggtgccct gcagctcgcc ggtgatgacg
      721 aggcggccgg ctgcgtcgat cgccaggtcg cgtccggtct gcagctgcgc gttgccgaag
      781 cgcttgctcc acacggcggt gcccttgggg tcgaggcgcg cgaggaagat gtccgcgtcg
      841 ccggcgctga ccagcggccc gttgccgaag ttggtcgtgc cctggaagcc gccggtcatg
      901 aacacgttgc cggcgaggtc ggtggcgagg ccctggacgt actggccctg ggggtcaccg
      961 aatcgctggc tccacaggtg cttgccgtcg gggtcgaact tgccgacaaa ggcgtcggcg
     1021 aggccggcgg aggtgagcat gccgccgccg aggtcgaggc cgctgacgaa gtagccgccg
     1081 agcaggatgc tgccggtgtc gtcgcgggtg accgcgccgg ggaccgcgta gccgttgggc
     1141 gcgtgaaaca ccttgctcca cacgtgtgtg ccgtcggcgg cgagcttgac gaggaacagg
     1201 tcgccgctgc tctgggcgtc gagcgggccg ccgccgaagt cgatcttgcc ctcgaagtcg
     1261 ccgctgagca cgatctcccc ggtggcggcg acgtcgaggg cgtagccgtc gtcgaactgg
     1321 ttggcggcgt cgccgaagcg cttgctccac aggtgcgcgc cgtcgggggc gaacctggcg
     1381 agcaggaggt cgtagctgcc cgcgcccgcc agcaggccgc cgccgaggtc gacgtcgccc
     1441 ttcgagtggg cggcgagcac gatgttgccc gcgccgtcga acgcgacccg caggccgttc
     1501 tcgcggctcg agctgccgaa cgcgtggtcc cactgatagg tgccggtgcc gccgcaggcg
     1561 tcgacgccgt cgcagttctc gtcgcccggg gtcacgcagc tctcggcggc cggcaacacc
     1621 tcgccgaggc acggggccca ctcgccctgc ggcgtgcaca gctgctcgcc ggcggcgcag
     1681 aggccgatgc cctcggtgcc gggcgggccc ccgtagcacg gctgggtgtc gcccggcgtg
     1741 cagtcgcccg gcatgccggt ggtgt
//
LOCUS       DASWNJ010001258         4243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_444634, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001258 DASWNJ010000000
VERSION     DASWNJ010001258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444634"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..659
                     /locus_tag="VGB85_21145"
     CDS             75..659
                     /locus_tag="VGB85_21145"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY0136609.1"
                     /translation="MPAPAVRRATYQDVLDAPENMIAEVLYGTLYTMPRPAFSHARVS
                     SRLGVALGKHFDDEDGPTGWLLLDEPELHLGSEPDILVPDLAGWRLARVQPGMDDAPW
                     TSIAPDWICEVLSPATHVRDRGIKLDIYLRERVGHVWLVDPIAETLEIFRHNGTQWSR
                     GSLHTGNVTVRAEPFDAIELELARLWKRRAPPQP"
     gene            710..1279
                     /locus_tag="VGB85_21150"
     CDS             710..1279
                     /locus_tag="VGB85_21150"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY0136610.1"
                     /translation="MPAPAVRRATYQDVLDAPPNTIAEVIFGTLHTQPRPAFAHGDVT
                     SNLGAIVVTRFGFGDGGPGGLIIRDEPELHLGAEPDILVPDLAGWHRERLRALPENAP
                     WTNIAPDWVCEVLSPSTQALERNEKMAIYLRERVTHVWLIDPLARILEVYRHDGPHWA
                     RIATHQAGETVRAEPFDATEIQLRRLWER"
     gene            complement(1337..3016)
                     /gene="ettA"
                     /locus_tag="VGB85_21155"
     CDS             complement(1337..3016)
                     /gene="ettA"
                     /locus_tag="VGB85_21155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEY0136611.1"
                     /translation="MVAYQYIYTMKDLRKVYPSGKEVLKGIYLSFFPGAKIGVLGANG
                     AGKSTLLKIMAGVDKEFLGEAWAAEGVSVGYLPQEPALDPKKTVLGNIEEGVSVMKGV
                     LTRFEEVSMKLGEPMSDAEMNRLLSEQAELQDKIDAGNGWELDRTLEIAMDALRCPPG
                     DADVTTLSGGERRRVALCRLLLQRPDMLLLDEPTNHLDAESVAWLERFLQEYPGTVVA
                     ITHDRYFLDNVAGWILELDRGAGIPWKGNYSSWLDQKGKRLAQEEKAESNRQRTLARE
                     LEWVQMSPKARQAKSKARVSAYEALLAKSDDKRTDTTELTIPFTRRLGNTVIEAKKLV
                     KGFGERLLFDDLNFTLPRGGIVGIIGPNGAGKTTLFRLITGTEKPDRGDLKIGDTVDI
                     AAVDQSRDTLDPDKSIYQEISGGQEVLSVGGRDINARAYVSKFNFNGADQQKKVGLLS
                     GGERNRVHLAKLIKKGGNLLLLDEPTNDLDVDTLRHLEDALLGFPGCAVIISHDRWFL
                     NRLATHILAFEGESKVVWFEGNYEDYEADKKRRLGEEALTPHRIKYRKLVH"
     gene            3224..>4243
                     /locus_tag="VGB85_21160"
     CDS             3224..>4243
                     /locus_tag="VGB85_21160"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136612.1"
                     /translation="MSHASEVEAKSEARSKSEGADTLPPSSGVVPGSTPGTNLPAFEA
                     AGAAVEADKRAAQSHALAVDASFKRYGLDRWGKDFLRSNEHGHLVFTAPNCPPVDLHA
                     LSVELARRGIRTPYVIRFPTMIEKQMDTLKSAFAKAIAENSYQGGHIGVYPLKVNQRR
                     SVVDVVVKARERLNYGMEAGSKPELLLAMAQPALKQSLLVCNGYKDREFMRMAFHAAE
                     LGHHVIIVLESTREVRRYLDVLNEQDWIARPEVGVRAKLYSRGSGRWQSSGGESSKFG
                     LTTNEILAVVRELASANMQGQLSLLHFHIGSQITQIKRIKTVVREGARIYADLQEHCP
                     QLRYLD"
BASE COUNT          652 a         1520 c         1371 g          700 t
ORIGIN      
        1 ggtcctcgcc gagcgcctcg agatccacta aaggcccgcg gcgccgcgcg cgagcctctg
       61 ttacgctgac cgtgatgccc gcgcccgccg tccgtcgcgc cacctaccag gacgtcctcg
      121 acgcccccga gaacatgatc gcggaggtgc tctacgggac gctatataca atgccgcgcc
      181 cggcgttctc tcatgcacgg gtgagctcgc gcctcggtgt cgcgctcggc aaacacttcg
      241 acgacgaaga cggtccgacc gggtggcttc tcctcgatga gcccgagttg cacctcggca
      301 gcgagcccga catcctcgtg cccgacctcg caggctggcg actggcgcgg gtgcagccgg
      361 gcatggacga tgcgccatgg acctccatcg cgccggactg gatctgcgag gtgctctcgc
      421 ccgcgacgca cgtccgcgac cgcggcatca agctcgacat ctatctccgc gagcgcgtcg
      481 gccacgtctg gctcgtcgac ccgatcgccg agaccctcga gatcttccgc cacaacggca
      541 cccagtggag ccgcggctcg ctgcacaccg gcaacgtcac cgtccgcgcc gagcccttcg
      601 acgccatcga actcgagctc gcccgcctct ggaaacgccg cgccccaccg cagccctgag
      661 gccgcgcacg catgcgccgt ggcctggtgt ctgttaggct gacgaggtca tgcccgcgcc
      721 cgccgtacgc cgcgccacct atcaggacgt cctcgacgcc ccgccgaaca cgatcgcgga
      781 ggtcatcttc ggcacgctgc atacccagcc gcgcccagct ttcgcccacg gtgatgtcac
      841 ctcgaacctc ggtgccatcg tcgtcactcg ctttgggttt ggcgacggcg gtccgggcgg
      901 gttgatcatt cgcgatgagc ccgagctgca cctgggcgcc gaacccgaca tcctcgtgcc
      961 cgatctggcc ggatggcacc gcgagcgtct gcgggcactc ccagagaatg ccccgtggac
     1021 aaatattgct ccggactggg tctgcgaggt cctctcaccc tcgacccagg cgctcgaacg
     1081 caacgagaag atggccatct atctgcgcga acgcgtcacc catgtctggc tcatcgatcc
     1141 tctggcccgg atcctcgagg tgtaccgtca cgacggcccc cactgggcac gtatcgctac
     1201 ccatcaggct ggcgagaccg tacgcgctga gcccttcgat gccaccgaga tccagctccg
     1261 gcgtctgtgg gagcgctgac ttgaacgaaa acgcggccgc tctctcgagc cggccgcgcc
     1321 tgtcctcgcg gacagctcag tgtacgagct tgcggtactt gatgcggtgg ggcgtcagcg
     1381 cctcttcgcc gaggcggcgc ttcttgtcgg cctcgtaatc ttcatagttg ccctcgaacc
     1441 acacgacctt ggactcgccc tcgaaggcga ggatgtgggt ggcgaggcgg ttgaggaacc
     1501 agcgatcgtg gctgatgatg acggcgcagc cggggaagcc aagcagcgcg tcctcgaggt
     1561 ggcgcagggt gtcgacgtcg aggtcgttgg tcggctcgtc gagcaacagc aagttgccac
     1621 cctttttgat cagcttggcg aggtggacgc ggttgcgctc gccgcccgac agcaggccga
     1681 ccttcttctg ctggtcggcg ccgttgaagt tgaacttgct gacgtacgcg cgcgcgttga
     1741 tgtcacgccc gccgacgctg agcacctcct ggccgccgga gatctcttga tagatcgact
     1801 tgtcggggtc gagcgtgtcg cggctctggt cgacggcggc gatgtcgacg gtgtcgccga
     1861 tcttgaggtc gccgcggtcg ggcttctcgg tgccggtgat caggcggaac agggtcgtct
     1921 tgccggcgcc gttcgggccg atgatgccga cgatgccgcc gcgcggcagc gtgaagttga
     1981 ggtcgtcgaa cagcaggcgc tcgccgaagc ccttgaccag cttcttggcc tcgatcaccg
     2041 tgttgccgag ccgccgcgtg aacgggatcg tcagctcggt cgtgtcggtg cgcttgtcgt
     2101 ccgacttggc gagcagcgcc tcgtaggcgc tgacgcgggc cttgctcttg gcctggcggg
     2161 ccttgggcga catctgcacc cactcgagct cgcgcgcgag cgtgcgctgg cggttgctct
     2221 cggccttctc ctcctgcgcg agccgcttgc ccttctggtc gagccacgac gagtagttgc
     2281 ccttccacgg gatccccgcc ccgcggtcga gctcgaggat ccagccggcg acgttgtcga
     2341 ggaagtaacg atcgtgggtg atcgccacca ccgtgcccgg atactcctgc aagaagcgct
     2401 cgagccacgc gacgctctcg gcgtcgagat ggttggtcgg ctcgtcgagc agcaacatgt
     2461 ccggacgctg cagcagcagc cggcacagcg cgacgcggcg ccgctcgccg cccgacagcg
     2521 tcgtgacgtc ggcgtcgccc ggcgggcagc gcagcgcgtc catcgcgatc tcgagcgtgc
     2581 ggtcgagctc ccagccgttg ccggcgtcga tcttgtcctg aagctcggcc tgctcgctga
     2641 gcaggcggtt catctcggcg tcgctcatcg gctcgccgag cttcatgctg acctcctcga
     2701 agcgagtcag cacgcccttc atgaccgaga cgccctcctc gatgttgccg aggaccgtct
     2761 tcttggggtc gagcgcgggc tcttgcggca ggtatccgac gctgacgccc tccgccgccc
     2821 aggcctcgcc gaggaactcc ttgtcgacgc cggccatgat cttgagcagc gtcgacttgc
     2881 cggccccgtt cgcgcccagc acgccgatct tcgcccccgg gaagaacgaa aggtagatgc
     2941 ccttgagcac ctccttgccg gaggggtaga ccttgcgcag gtccttcatc gtgtagatgt
     3001 actgataggc gaccatggct ctcggcgtct cctcggcggc ccgtgccagc gatctgctgg
     3061 ctcaatcatg gctgggcgcg cgcggcggga ggataccagc accgtgacgc gaccgcgcgc
     3121 ctgtctgcgc cgcgacggcg ccgcaaaacc tgtgtgaaat ctcgggcccg cggccggccg
     3181 ggggctggtg ccccgcgaac accgctccca taatccgggc ccgatgagcc acgcatccga
     3241 agtcgaagcc aagtccgagg cacgcagcaa gtccgagggc gccgacacgc tcccgcccag
     3301 ctccggcgtc gtccccggga gtaccccggg taccaacctc cccgccttcg aggccgccgg
     3361 cgccgccgtc gaggccgaca agcgggccgc ccagagccac gcgctggcgg tcgacgcctc
     3421 gttcaagcgt tacggcctcg accggtgggg caaggacttc ctgcggagca acgagcacgg
     3481 ccacctggtg ttcacggccc cgaactgccc gccggtggac ctccacgcgc tgtcggtcga
     3541 gctcgcccgg cgcggcattc gcacgccgta cgtgatccgc ttcccgacga tgatcgagaa
     3601 gcagatggac accctcaaga gcgccttcgc caaggcgatc gccgagaaca gctaccaggg
     3661 cggccacatc ggcgtgtacc cgctcaaggt caaccagcgg cgcagcgtcg tcgacgtcgt
     3721 cgtcaaggcg cgcgagcgcc tcaactacgg catggaggcc ggcagcaagc cggagctgct
     3781 gctggcgatg gcccagccgg cgctcaagca gtcgctgctg gtctgcaacg gctacaagga
     3841 ccgcgagttc atgcgcatgg ccttccacgc ggccgagctc gggcaccacg tcatcatcgt
     3901 gctcgagagc acccgcgagg tccgtcgcta cctcgacgtg ctcaacgagc aggactggat
     3961 cgcccggccc gaggtcggcg tgcgcgccaa gctgtacagc cgcggcagcg gccgctggca
     4021 gagctccggc ggcgagagct ccaagttcgg cctgacgacc aacgagatcc tcgcggtcgt
     4081 gcgcgagctc gccagcgcca acatgcaggg ccagctgtcg ctgctgcact tccacatcgg
     4141 ctcgcagatc acccagatca agcgcatcaa gaccgtcgtc cgcgagggcg cccgcatcta
     4201 cgccgacctg caggagcact gcccgcagct gcgctacctc gac
//
LOCUS       DASWNJ010001259         5042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_374413, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001259 DASWNJ010000000
VERSION     DASWNJ010001259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5042
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374413"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>5042)
                     /locus_tag="VGB85_21165"
     CDS             complement(<1..>5042)
                     /locus_tag="VGB85_21165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136613.1"
                     /translation="LLDHKDVQVQAAALLEPIYLDTQDHASRVKILEVRRAQAEQLGS
                     VDEATGYLLELARIHESDLDNPEAAFEAVRQAYLLEPRRSDIRSELQRLGIQLEKQRE
                     LVEVWQAALPKLPDAGAKIELLSRIAVVLDDHLQDQDGARRAYAELLALDPPDVELAR
                     RTTVALARLHRAAGDAPALVETLRGLLRFIDKDVEQVRVLLEIAELQRDRIEDSQAAA
                     GTYQEVLDIDPANMPAMDALERIFVAQGSWEPLTQVLRGRVMAVEDVSERARLWRKIG
                     EIQRDQLKDPYQAIEAFQAIVDLKAPTRETVTAIDAIVRLNAELERWPEVEEGLRSLL
                     RLADDDETRASLLGRTAAVVGDKLGRYPDAIDLLEQVLRISPEDERARSTLTEYLGND
                     EVRERVIGILHPIYEAEHNWPALLDLEERRARHLPPGRNRLLALLQVARTQEERLGDA
                     DRAFAVLGDALAEAGDQPELADILDRLERFDADPERGAALLDAYLKVVDGVQDPALQG
                     RVLRNIGRLAANQNRLDEARRAHERLLDQDPSDTAAADALEQVLVRQADDDALAELLV
                     RRAERTEGDERDRFFMRAAEIYRTRLSRPEDAIGLYERVRPDGAASAEAQTLLAPLYE
                     STGRWHDLTRFLEAKLDRQSGRERTENHLRLATIYRDQLGDLASGLRHFAEALRHEPE
                     YVSAGPDLERYFADEDLRPQVIEQLEPVFRAAQDWPRLIRVQELRLAEAGDDTAARVK
                     ILTAVAQIQEDMLENYDAGFDTYARIFKEDPTHKPARDQLTRLAHALARTEALAELLT
                     AHVTGDAAGDERDESLAIVREAADLWISLDKPARAVPLYERLIAARPNDPNVFPALEQ
                     ALTRAEMWKELAEAYWREADTSLDEQRQIQVLMRLSELALGILGDSELAIRAFVRVLE
                     TQPAEETARARLERVYENLARWDALIESLRQRLERADEMITREAIQLRIADLQDTRLD
                     NPDAAIDTLEALLGEQPGNISAIQALEQLAETRAEQRVRLFAILRPLYEQSQDAMRLV
                     AVNEWQLTQTEDAVTRHELLRETAALLEPQGPEGPEYAFNALVRALSEPGPEGALEAL
                     DAEILRVARQYGYDYDLRQAQLQAAESEPLQADVGRRVSLMLQAGQLALNAGDNTAAI
                     TILRRALEVSPDDRGALALLDEALAIEGDFEGLRAVLERRVEVATDDEERIDLLRRLA
                     RLVEQALHLPDAAEAAWRRILDIEPGNPDAMLRLRRLYAAKGDHAKLIGVLEQQIEST
                     DDPEARLGLRKELARVHREGTHDRPAEIEALRALLMDAPEDEEALTALTTAQLATENY
                     PEAATTLLERVNLAPRERKVALLLEVARLQTGPMADPAGAMPHYESIFAITPAQNDAV
                     ADLMRLVVQPTTSESAAALLIPQLDQAHRYAELAQVWEARANLAESSTDAVEALRQLA
                     RIRFERLGDIAGSLTANNLLMDRVGPEDLRPVLEASNKMSVRLDKAEEHVDALARRAA
                     RTDLDPQSRVMIAQSAADMAEGILGDRTRALHLLATLLDAEIADENTVRNIERLARPA
                     GDKQLLARALAESAKLAVGQPTHAEILVRLGDARHDIGDLAGATAAYAEALDDRPGFA
                     GAVAGLERVLERSQRTSVAIPPGLLEALDNAYHVAGNKPGLTRVARLRLNDATGADRV
                     AGLETLARLIDD"
BASE COUNT          583 a         1742 c         1945 g          772 t
ORIGIN      
        1 gtcgtcgatc aggcgcgcga gggtctcgag gccggcgacg cggtcggcac cggtcgcgtc
       61 gttgaggcgc aggcgagcga cccgcgtcag gcccggcttg ttgccggcga cgtggtacgc
      121 gttgtcgagc gcctcgagca ggcccggcgg gatcgccacg ctggtccgct gtgagcgctc
      181 gagcacccgc tcgagccccg cgaccgcgcc ggcgaagccc gggcggtcgt cgagcgcctc
      241 ggcgtaggcc gcggtcgcgc cggcgaggtc accgatgtcg tggcgggcgt cgccgaggcg
      301 cacgaggatc tccgcgtgcg tcggctgtcc aaccgcgagc ttggccgact cggcgagcgc
      361 ccgcgcgagc agctgcttgt cgcccgcggg ccgcgcgagc cgctcgatgt tgcggacggt
      421 gttctcgtcg gcgatctcgg cgtcgagcag cgtcgccagc aggtgcagcg cgcgcgtacg
      481 gtcgccgagg atgccctcgg ccatgtcggc ggccgactgc gcgatcatca cgcgcgactg
      541 cgggtcgagg tcggtgcgag ccgcccggcg cgcgagcgcg tcgacgtgct cctcggcctt
      601 gtcgaggcgc accgacatct tgttgctggc ctcgagcacg ggtcgcaggt cctcgggacc
      661 gaccctgtcc atcagcaggt tgttggcggt gaggctgcct gcgatgtcgc cgagccgctc
      721 gaagcggatg cgggcgagct ggcgcagcgc ctcgacggcg tcggtcgagg actcggccag
      781 gttggcgcgg gcctcccaca cctgcgcgag ctcggcgtag cggtgcgcct ggtcgagctg
      841 cgggatcagc agcgcggccg ccgactccga ggtcgtcggc tggaccacga ggcgcatcag
      901 gtccgcgacc gcgtcgtttt gcgccggcgt aatcgcgaag atcgactcgt agtgcggcat
      961 ggccccggcc gggtcggcca tcggcccggt ctgcaggcga gcgacctcga gcaacaacgc
     1021 gaccttgcgc tcgcgcggcg cgaggttcac ccgctcgagc agggtcgtcg cggcctcggg
     1081 gtagttctcg gtggcgagct gcgcggtggt cagggcggtc agggcctcct cgtcctccgg
     1141 cgcgtccatc agcagggcgc gcagggcctc gatctcggcc gggcggtcgt gcgtgccctc
     1201 gcggtggacg cgggcgagct ccttgcgcag gccgaggcgc gcctcggggt catcggtgct
     1261 ttcgatctgt tgctcgagca cgccgatcag cttcgcgtga tcgcccttgg cggcgtacag
     1321 ccggcgcagg cgcagcatcg cgtccgggtt gccgggctcg atgtcgagga tccgccgcca
     1381 ggcggcctcg gccgcgtcgg gcaggtgcag cgcctgctcg acgaggcgcg cgagccggcg
     1441 cagcaggtcg atgcgctcct cgtcgtcggt ggcgacctcg acgcggcgct cgagcacggc
     1501 gcgcaggccc tcgaagtcgc cctcgatcgc gagcgcctcg tcgagcagcg ccagcgcgcc
     1561 gcggtcgtcc gggctgacct cgagcgcgcg gcgcaggatc gtgatcgcgg ccgtgttgtc
     1621 gccggcgttg agcgcgagct gaccggcctg caacatcagc gagacgcggc ggccgacgtc
     1681 ggcctgcagc ggctcggact cggcggcctg gagctgcgcc tggcgcaggt cgtagtcgta
     1741 gccgtactgg cgagcgaccc gcaggatctc ggcgtcgagg gcctcgagcg cgccctccgg
     1801 gccgggctcg gacagcgccc gcacgagggc gttgaacgcg tactcggggc cctcggggcc
     1861 ctgcggctcg agcagcgcgg ccgtctcgcg cagcagctcg tgccgcgtca ccgcgtcctc
     1921 ggtctgggtc agctgccact cgttgacggc gaccaggcgc atcgcgtcct ggctctgctc
     1981 gtagagcggg cgcaggatcg cgaacaggcg cacgcgctgc tcggcgcgcg tctcggcgag
     2041 ttgctcgagc gcctggatcg cgctgatgtt gccgggctgc tcgccgagca gcgcctcgag
     2101 ggtgtcgatc gcggcgtcgg ggttgtcgag cctcgtgtcc tgcaggtcgg cgatccgcag
     2161 ctggatcgcc tcgcgggtga tcatctcgtc ggcccgctcg aggcgctggc gcagcgattc
     2221 gatcagggcg tcccagcggg ccaggttctc gtacacgcgc tcgaggcgag cgcgcgcggt
     2281 ctcctcggcg ggctgcgtct cgagcacgcg gacgaacgcg cggatcgcca gctcggagtc
     2341 gccgaggatg ccgagcgcga gctcgctgag gcgcatcagg acctggatct ggcgctgctc
     2401 gtcgaggctg gtgtcggcct cgcgccagta cgcctcggcg agctccttcc acatctcggc
     2461 gcgggtcagg gcctgctcga gcgcggggaa cacgttcggg tcgttcgggc gcgcggcgat
     2521 caaacgctcg tacagcggca ccgcgcgggc gggcttgtcg aggctgatcc acaggtcggc
     2581 ggcctcgcgg acgatcgcca gcgactcgtc gcgctcgtcg ccggcggcgt cgccggtgac
     2641 gtgcgccgtc agcagctcgg cgagcgcctc ggtgcgcgcg agcgcgtggg ccagccgggt
     2701 cagctgatcg cgcgcgggct tgtgggtcgg gtcctccttg aagatgcggg cgtaggtgtc
     2761 gaagcccgcg tcgtagttct cgagcatgtc ctcctggatc tgcgcgacgg cggtcaggat
     2821 cttcacgcga gccgcggtgt cgtcaccggc ctcggccagc cgcagctcct gcacgcggat
     2881 cagccgcggc cagtcctggg ccgcgcggaa caccggctcg agctgttcga tcacctgcgg
     2941 gcgcaggtcc tcgtcggcga agtagcgctc gaggtccggg cccgccgaga cgtactcggg
     3001 ctcgtggcgc agcgcctcgg cgaagtggcg taaaccactg gcgaggtcgc cgagctgatc
     3061 gcgatagatc gtcgccaggc gcaggtggtt ctcggtgcgc tcgcggccgc tctgccgatc
     3121 gagcttggcc tcgaggaagc gggtcaggtc gtgccagcgg ccggtcgact cgtacagcgg
     3181 cgcgagcagg gtctgcgcct cggcactcgc ggcgccgtcg ggccgcacgc gctcgtacag
     3241 gccgatcgcg tcctcggggc ggctcaggcg ggtgcggtag atctccgcgg cgcgcatgaa
     3301 gaagcggtcg cgctcgtcgc cctcagtgcg ctcggcgcgg cgcaccagca gctcggcgag
     3361 cgcgtcgtcg tcggcctggc gcacgagcac ctgctcgagc gcgtcggcgg cggccgtgtc
     3421 gctcgggtcc tggtcgagca gccgctcgtg ggcccggcgc gcctcgtcga ggcgattttg
     3481 attcgcggcg agacggccga tgttgcgcaa taccctgccc tgcagcgcgg ggtcctgcac
     3541 gccgtcgacg accttgaggt aggcgtcgag caacgcggcg ccgcgctcgg ggtcggcgtc
     3601 gaagcgctcg aggcgatcga ggatgtcggc gagctcgggc tgatcaccgg cctcggccag
     3661 cgcgtcaccg agcaccgcga acgcccggtc ggcgtcgccg aggcgctcct cctgcgtgcg
     3721 cgcgacctgc agcagcgcga gcaggcgatt gcgtccgggc gggaggtgcc gggcgcggcg
     3781 ctcctcgagg tcgagcagcg cgggccagtt gtgctccgcc tcgtagatcg ggtgcaagat
     3841 gccgatcacg cgctcgcgga cctcgtcgtt gccgaggtac tcggtcaggg tgctgcgggc
     3901 gcgctcgtcc tcggggctga tccgcaggac ctgctcgagc aggtcgatcg cgtcggggta
     3961 gcgcccgagc ttgtcgccga cgaccgccgc ggtacgtccg agcagcgacg cgcgcgtctc
     4021 gtcgtcgtcg gcgagcctga gcaggctacg caggccctcc tcgacctccg gccagcgctc
     4081 gagctcggcg ttgagccgga cgatcgcgtc gatcgccgtg acggtctcac gcgtcggcgc
     4141 cttgaggtcg acgatcgcct ggaacgcctc gatcgcctgg tacgggtcct tgagctggtc
     4201 gcgctggatc tcgccgatct tgcgccacag gcgggcgcgc tcgctgacgt cctcgacggc
     4261 catcacgcgg ccgcgcagca cctgcgtcag cggctcccag ctgccctgcg cgacgaagat
     4321 gcgctcgagg gcgtccatcg ccggcatgtt cgcggggtcg atgtcgagga cctcctggta
     4381 ggtgccggcg gcggcctggc tgtcctcgat gcggtcgcgc tgcagctcgg cgatctcgag
     4441 cagcacgcgg acctgctcga catccttgtc gatgaagcgc agcaaaccgc gcagggtctc
     4501 gacgagcgcc ggggcgtcgc ccgcggcgcg gtgcaggcgg gcgagcgcga cggtggtgcg
     4561 gcgcgcgagc tcgacgtccg gggggtcgag cgcgagcagc tcggcgtagg cccggcgggc
     4621 gccgtcctgg tcctgcaggt ggtcgtcgag gaccacggcg atgcgcgaga gcagctcgat
     4681 cttcgcgccc gcgtcgggca gcttgggcag cgcggcctgc cacacctcga cgagctcgcg
     4741 ctgcttctcg agctggatgc cgaggcgctg gagctcgctg cggatgtcgc tgcggcgcgg
     4801 ctcgagcagg taggcctggc gcacggcctc gaaggcggcc tcggggttgt cgaggtcgct
     4861 ctcgtggatc cgggccagct cgagcaggta gccggtcgcc tcgtcgaccg agccgagctg
     4921 ctcggcctgg gcgcggcgga cctcgaggat cttcacgcgg ctcgcgtggt cctgggtgtc
     4981 gaggtagatc ggctcgagca gcgcggcggc ctgcacctgc acgtccttgt ggtcgagcag
     5041 gc
//
LOCUS       DASWNJ010001260         1877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_587904, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001260 DASWNJ010000000
VERSION     DASWNJ010001260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587904"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..1466
                     /gene="tssK"
                     /locus_tag="VGB85_21170"
     CDS             102..1466
                     /gene="tssK"
                     /locus_tag="VGB85_21170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HEY0136614.1"
                     /translation="MADNSLQPVVWKDGVLIAPQHFQQQDRHHAASLHARLAALSPQA
                     WGVSALTLDETALRTGVVKVNEFRGILPDGLIAAFRDGEPGAPPSRPLAEHFPATART
                     LDVAIGAPLLRDGIENFAGNEYARYRTVVRNDVPDLTGARSKVDLELGQPNLSILLGA
                     EIQQSGLAAVKIGEVVREAAGSYAWNSHYIPPCLTISVSRHLTSGLKDLLGAAIARRR
                     ALVEIRRERDGEVAEFLARDITNYLLLDALGSNIPLIKHLVDTPSTPPLAAHLALLQF
                     AGALTSFATAVDPAELPSFNYLDLRASFDELLRQIRAMLGLQLREKFVKIPLTLRQED
                     GMWLGRLSDDSMLGCSSYVLAVESALPVNELATTLPRLSKLASWKKIYDHIKLATPGV
                     RISWLKTPPPELPARPRQVYFVINTTDPDWRAITSERTIAAFFPDPFEPEQLRVELFG
                     ILGT"
     gene            1478..>1877
                     /locus_tag="VGB85_21175"
     CDS             1478..>1877
                     /locus_tag="VGB85_21175"
                     /inference="COORDINATES: protein motif:HMM:NF021377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HEY0136615.1"
                     /translation="MELVTQIRAITARCFTAVELLRRIDTGATHTFERLHEHLMTDLE
                     AAERLASQAFGPVVAEDIKYALVALSDETALRQPGPLRDFWKPRSLQLHYFSDNRAGF
                     NFFERLGEIQSDPGRHPALPVYALCLQFGLR"
BASE COUNT          281 a          793 c          529 g          274 t
ORIGIN      
        1 gtgcccccgg cgcgggcgct gccccgaccg gcgctgcacc ttcgggcgcc ggcggtgtcc
       61 cctccactcc ctccgcgcca cgtcgcccct tctgagagcc catggccgac aacagcctgc
      121 aaccggtcgt gtggaaggac ggcgtgctca tcgcgccgca gcactttcaa caacaggacc
      181 gccaccacgc cgccagcctg cacgcccgcc tcgccgccct ctcgccgcag gcgtggggcg
      241 tctcggcgct gaccctcgac gagaccgccc tgcgcaccgg cgtcgtcaag gtcaacgagt
      301 tccgcggcat cctgcccgac ggcctgatcg ccgccttccg ggacggtgag cccggcgccc
      361 cgccctcgcg tccgctcgcc gagcacttcc ccgccaccgc gcgcaccctc gacgtcgcca
      421 tcggcgcccc gctgctgcgc gacggcatcg agaacttcgc cggcaacgag tatgcccgct
      481 accgcaccgt cgtccgcaac gacgtccccg acctcaccgg cgcgcgcagt aaagtggacc
      541 tcgagctcgg ccagcccaac ctctcgatcc tgctcggcgc cgagatccag cagagcgggc
      601 tcgctgccgt gaagatcggc gaggtcgtgc gcgaggccgc cggcagctac gcgtggaaca
      661 gccactatat cccgccctgc ctgaccatct cggtctcccg ccacctgacc agcggcctca
      721 aggacctgct cggcgccgcg atcgcccgcc gccgcgcgct cgtcgagatc cgccgcgagc
      781 gtgacggcga ggtcgccgag ttcctcgccc gcgacatcac caactacctg ttgctcgacg
      841 ccctcggcag caacatccca ctaattaaac acctcgtcga cactccctcg accccgccgc
      901 tggccgccca cctcgcgctg ctgcaattcg ccggcgcact caccagcttc gccaccgccg
      961 tcgaccccgc cgagctgccc tcgttcaact acctcgacct gcgcgccagc ttcgacgagc
     1021 tcctgcgcca gatccgggcg atgctcggcc tgcagctgcg cgaaaagttc gtcaagatcc
     1081 ccctgaccct gcgccaggag gacggcatgt ggctcggccg cctcagcgac gacagcatgc
     1141 tcggctgctc gagctacgtg ctcgccgtcg agtccgccct gcccgtcaac gagctcgcca
     1201 cgacgctccc gcgcctctcg aagctcgcct cgtggaagaa gatctacgac cacatcaagc
     1261 tcgccacacc gggcgtgcgc atcagctggc tcaagacccc gccgccggag ctgcccgcgc
     1321 gcccgcgcca ggtctacttc gtcatcaaca ccaccgaccc cgactggcgc gcgatcacca
     1381 gcgaacgcac gatcgccgcc ttcttccccg accccttcga gcccgagcag ctgcgcgtcg
     1441 agctgttcgg catcctcggc acctgacctg atcatcgatg gagctcgtca cccagatccg
     1501 cgcgatcacg gcccgctgct tcaccgcggt ggagctgctg cgtcgcatcg acaccggcgc
     1561 cacccacacc ttcgagcgcc tccacgagca cctgatgacc gacctcgagg ccgccgagcg
     1621 cctcgccagc caggcatttg gtcccgtggt cgccgaggac atcaagtacg cgctcgtcgc
     1681 cctctccgac gagaccgccc tgcgccagcc cggcccgctg cgcgacttct ggaagccccg
     1741 ctcgttgcag ctgcactact tcagcgacaa ccgggccggc ttcaatttct tcgagcgcct
     1801 cggcgagatc cagagcgacc ccggccgtca ccccgcgctc ccggtctacg cgctgtgcct
     1861 gcagttcggc ctgcgcg
//
LOCUS       DASWNJ010001261         1958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_92851, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001261 DASWNJ010000000
VERSION     DASWNJ010001261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92851"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1478
                     /locus_tag="VGB85_21180"
     CDS             <1..1478
                     /locus_tag="VGB85_21180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0136616.1"
                     /translation="ERRKAEDELRASEERFRMLVSGVRDYAIFLLDPTGHVVTWNVGA
                     ERIKGYTAAEIVGKHFSTFYTPEDLIAGKAQRELEVASRDGSYEEEGWRVRKDGSKLW
                     ASVVITALRDGQGRLRGFSKVTRDITERRESERIKSIVDNVVDGIVTFDENGTIESLN
                     PAAERVFGYQAAELVGTAIARLAAEGDKLDWIGPSEGGDIREVFGRRKDGAAFPMDLA
                     IGPFHFQGRRAYTAVVRDITERRKDEERLRFYAQELRDKNAELARSNQELDDFAYIAS
                     HDLKEPLRGIHNYANFLLEDYADKLDADGRAKLETLTLLAMRMEALIDSLLQFSRVGR
                     VELSNQETDLNEVVRMVLESLQITVREEAIDVRIPRALPTVRCDQVRVREVFHNLVIN
                     AIKYNDKPHRWVEIGWQDGGGGPPVFHVRDNGIGMQQKHHDAIFRMFKRLHGRDKYGG
                     GTGVGLTIVKKIVERHGGRIRVESTVGEGTTFYFTLAPEAA"
     gene            1475..1924
                     /locus_tag="VGB85_21185"
     CDS             1475..1924
                     /locus_tag="VGB85_21185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0136617.1"
                     /translation="MNLGPRQTILLVEDSPEDFEATVRALRRSGLANPIARCEDGDEA
                     LDYLHRRGRFCDPATSPRPGIILLDLNLPGTDGREVLADLKGDVGLRSIPVIILTTSI
                     DERDVERCYSIGANSYMKKPVDLDGFMRAIQRLKEFWFEVVIVPRPE"
BASE COUNT          353 a          688 c          649 g          268 t
ORIGIN      
        1 gcgagcgccg caaggccgag gacgagctgc gggccagcga ggagcgcttc cggatgctcg
       61 tcagcggggt ccgggactac gcgatcttcc tgctcgaccc caccggacac gtcgtcacct
      121 ggaacgtcgg cgccgagcgg atcaagggct acaccgccgc ggagatcgtc ggcaagcact
      181 tctcgacctt ctacaccccg gaggacctga tcgccggcaa ggcgcagagg gagctcgagg
      241 tcgcgtcgcg cgacggcagc tacgaggagg agggctggcg cgtccgcaag gacggctcga
      301 agctgtgggc cagcgtcgtc atcaccgccc tgcgcgacgg ccagggccgc ctgcgcggct
      361 tctcgaaggt cacgcgcgac atcaccgagc ggcgagagag cgagcggatc aagtcgatcg
      421 tcgacaacgt cgtcgacggc atcgtcacct tcgacgaaaa cggcacgatc gagagcctca
      481 accccgcggc cgagcgcgtc ttcggctacc aggccgccga gctggtcggc accgcgatcg
      541 cgcggctcgc cgccgagggc gacaagctcg actggatcgg tccgagcgag ggcggcgaca
      601 tccgcgaggt gttcggccgg cgcaaggacg gcgccgcctt cccgatggac ctcgcgatcg
      661 gcccgttcca cttccagggc cggcgcgcct acacggcggt tgtccgcgac atcaccgagc
      721 ggcgcaagga cgaggagcgc ctgcgcttct acgcccagga gctgcgcgac aagaacgccg
      781 agctggcgcg cagcaatcaa gagctcgacg acttcgcgta catcgccagc cacgacctca
      841 aggagccgct gcgcgggatc cacaactacg ccaacttctt gctcgaggac tacgccgaca
      901 agctcgacgc cgacggccgc gccaagctcg agacgctgac cctgctcgcg atgcgaatgg
      961 aggcgctgat cgactcgctg ctgcagttct cgcgggtcgg ccgcgtcgag ctgtcgaacc
     1021 aggagaccga cctcaacgag gtcgtgcgca tggtgctcga gtcgctgcag atcacggtgc
     1081 gcgaggaggc gatcgacgtc cgcatcccac gcgcgctgcc gaccgtccgc tgcgaccagg
     1141 tgcgcgtgcg cgaggtgttc cacaacctcg tcatcaacgc gatcaaatac aacgacaagc
     1201 cgcacaggtg ggtcgagatc ggctggcagg acgggggcgg cggcccgccc gtgtttcatg
     1261 tccgcgacaa cggcatcggc atgcaacaaa agcatcacga cgcgatcttt cgcatgttca
     1321 agcgcctgca cggccgcgac aagtacggcg gcggcaccgg cgtcggcctg acgatcgtca
     1381 agaagatcgt cgagcgccac ggcggtcgga tccgcgtcga gtcgaccgtc ggcgagggga
     1441 ccacgttcta cttcacgctc gcgccggagg ccgcatgaac ctcggacccc ggcagacgat
     1501 cctgctcgtc gaggacagcc ccgaggactt cgaggcgacc gtccgggccc tgcggcgctc
     1561 cgggctcgcc aacccgatcg cgcgctgcga ggacggcgac gaggcgctcg actacctcca
     1621 ccgccgcggc cgcttctgcg atccggcgac ctcgccgcgg cccggcatca tcctgctcga
     1681 cctcaacctg cccggcaccg atggacgcga ggtgctcgcg gacctcaagg gcgatgtcgg
     1741 cctgcgatcg atcccggtga tcattttgac gacctcgatc gacgagcggg acgtcgagcg
     1801 ctgctacagc atcggcgcca acagctacat gaagaagccg gtcgacctcg acggcttcat
     1861 gcgcgcgatc cagcggctca aggagttctg gttcgaggtc gtcatcgtgc cacgcccgga
     1921 ataacccgct cgcatgtccg acccccgcaa acgactgc
//
LOCUS       DASWNJ010001262         9980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_306273, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001262 DASWNJ010000000
VERSION     DASWNJ010001262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 9980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9980
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306273"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1885)
                     /locus_tag="VGB85_21190"
     CDS             complement(29..1885)
                     /locus_tag="VGB85_21190"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0136618.1"
                     /translation="MNLVLRFFKRYVRRNLPQYALGAVMLVITNYAVVRIPTLIGGAL
                     DALGPNGAGMAAGRSLAVELMFWALAVVIVRTLSRVLFFNPGRDVEFRLGVDLFTHLL
                     AMQRPFYASRRVGELVSMATNDTMAVRLLVGFAGLQVFNVAVAIPMHLIQMWATDPVL
                     TLWCLGPIALGAFYTQLVVRQFYAMIRVSFEQLARLSDRVLESYAGIGTARAHAAEEA
                     LLRRFDERNQPYLDLQLKLSSQRAFAMPVLGLSGLVATALVLWVGGDRVIDGEMQVGA
                     IATFTTLLASLVALLMALAWVLASISRGLIAISRIDQVIQTPDDLPVPTASLSLPRPP
                     SLALRSLSFTYPGADGPALQDIDVSLEPGQTLGIFGRTGAGKSTLINLLARVYTPPAG
                     SVLVDGHDVTSVPLADLRAGLAVVPQDPFLFSTTLRDNIRLQGERSSHTRGEDAEPDR
                     SGARKPIPAAIADDPLLRTVLASACLDDDIRALPEGLDTVVGERGVTLSGGQRQRTAL
                     ARALYRRPALLLLDDVLSAVDQGTELKLVAAIRQLHEAPIPVGQARERTTGPGLPPTT
                     VIVSHRTSVLEHADEILVLEAGKVVERGTHQSLLAQNGLYAETHRHQEAARE"
     gene            complement(2049..2294)
                     /locus_tag="VGB85_21195"
     CDS             complement(2049..2294)
                     /locus_tag="VGB85_21195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136619.1"
                     /translation="MKKATIAFVFAALSVGTMGCNKSKADTATPEDGTKTDTPPPSDA
                     TTPADGEGAGAGDAGAGAGDAGGAGDAAGDTAAAPTP"
     gene            complement(2525..3148)
                     /locus_tag="VGB85_21200"
     CDS             complement(2525..3148)
                     /locus_tag="VGB85_21200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136620.1"
                     /translation="MRRPATLALIVLISRVDLACNEPTPGRAVAAPLPTRSDPPEPPP
                     MTPVELAEARRKAGFRDDDLAAEKAHAREQGGRQYVREHLAAYRGLVKALRRSVDDID
                     RSARRWASSRDPQRSYRRWREARGRMFADLSKRYREVRREGVDAGSTQALIERIYGAW
                     EDVHNDLGGDAAEQERFTAVIRELRDGLDAAEAALDDIEHDEDLEGK"
     gene            3262..4140
                     /locus_tag="VGB85_21205"
     CDS             3262..4140
                     /locus_tag="VGB85_21205"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HEY0136621.1"
                     /translation="MSNPAATLEVLQAAAGVLAGHGGPAALATVIARSGSAPQVVGAR
                     LLLRADGSMVGTVGGGAIEAQVLEACRTTLREGTPSVVKAHLVRDLGMCCGGSMEVFV
                     EYLDAQARLVLIGGGHVAQALAPVARAVGFRVVVVDDREDMLGHPAFAADRRELADVD
                     ELAAAVPDLDERDYLVIATRDHARDEQALAELLRRPHRYIGMIGSRRKVHAIVARIVR
                     REQALGRGAPDLGRLRAPVGLALGGRTPAEIAVSIVAELLAERHGGSGAPMNVVSQAI
                     SRIDRDLAAAPDEAAE"
     gene            4293..6116
                     /locus_tag="VGB85_21210"
     CDS             4293..6116
                     /locus_tag="VGB85_21210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136622.1"
                     /translation="MSRLLPAKIVAVTAVLLTVPACTHKKPPPTAVVVDDSSSDQGGG
                     TISEPADGTRQPQRTGKPIQVRASVEGLGDLLALVKQATTAWNPKNPIDANAWIQAAL
                     LQFGYGPGMWNSLDLNGVMAVDTAFYPDISTADMRLIGSVAATNPKGIMEGMPSGRRP
                     QPLGNGLWELLHGDLRMVLREQPKALEFATTQPDLDRASALGKSAAGGRRFKIHASDL
                     PPGMLSVDGVAAIPAGLRRQVSAVLREATRASFELDAGTDRDLTLELAADAPFERLGL
                     SPLGAARTQTTGLEGRLPGGAAIVVAMPWGSPELLHKMVDNAAKQASQLGGAFETQVR
                     SVITASHSVLDQIQNDVVFAVYPGPKGEMTILIAADVKDEAAVRKAAHDILTTAGAGV
                     AAFNALGGDKKEASFTVTLKDKGIKSGAVEGELFTLGLPKNMANDLEDARPLLTSKNQ
                     IELASVVSGGSAVITIGFDAKKLAGDVAGGLKSARKTSLAADGGLVLARSASQGCHFC
                     VGIDPVGFARVAVFADKSMRADKARNKDLDAAATVVSRLGGALGFGLRLEPGKGALAG
                     GLKKSLLVLSPADAAQVGKLWESMSPAPAAEDKPLSGSRAK"
     gene            6166..6741
                     /locus_tag="VGB85_21215"
     CDS             6166..6741
                     /locus_tag="VGB85_21215"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY0136623.1"
                     /translation="MIATPAPAAVVLAAGASTRMGRPKALLEHHGRSFVACAVDLAAR
                     AGCAPIVVVTGAQELDGLGLAARLVHNENWQRGQLSSLQTGLAALEDPAGVLVLTVDR
                     PHLRATTVVTLVAAFRTAPQFSWQPAHAGRRGHPVIYPRSLLPMLAALPTSADPRELL
                     RAHEALRHTIAVDDPAVHDNLDRPEDLARLP"
     gene            6764..7600
                     /locus_tag="VGB85_21220"
     CDS             6764..7600
                     /locus_tag="VGB85_21220"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY0136624.1"
                     /translation="MDLQRIAPLHPDFDPALGLLRLDLEHGKANEIGGVELDAFEALC
                     DLLEAESEVSCLCTTSRRVSGRGTPIFISGANVSERVGWTDDRVKLHVVRQRSIMQRL
                     RHAPLYTIVLTHGVTFGWGAEFVLTADYCLATPTARFALPETGLGILPGARGTAELAS
                     LVGPAQALRLGCTGEEIGADEALRIGLVHETVADLEAGLARVRDVARRLRSRSPTAVA
                     AFKRGLLGALGRPEAERLAIERAAYEQCIDTGEAAIGRTAFSARRASGEAEVPPWGPR
                     RL"
     gene            7874..>9980
                     /locus_tag="VGB85_21225"
     CDS             7874..>9980
                     /locus_tag="VGB85_21225"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0136625.1"
                     /translation="MNLPDAHNKSTAGDEAEQPPMPERIAGRYQIRGMLGRGGFGAVY
                     EAYDELEDRPVALKVIRRDASLDPPGSRSSELPSATIAPASHAHSTRHLDSLRKAQVT
                     RSFGNTQGVPGDDITLAFKAEFRLLTQLHHPNLAAVYDFGRCEDEMVYFTQELLSGQS
                     LQDYLRSASRESIVEIFVQLARALDYIHTLGFVHEDIKPSNVIVCQPDTDGSQPQAKL
                     IDFGLARIFRRQKVASKLDDEFAVLGTPGFSAPEKLKGEPTDARSDIYSLAATIYTAV
                     RGSRPFPSRTFKEALRNQIDWRPELAGALLQQAGPVVAELVGRMLSPDPNVRPQSARS
                     IVLELLRRESVHIRDRQKTAQDRREFARVLVEHLPFVDRADYLDILLVRASEVLRPES
                     TRVAARGKPIRTILIEAPEGMGKARLMAELRREVQLGDGVFVEGSCWTSDGDGLGPFA
                     PVVLQLATALGERSLTVKQYGDLVRAARERSHETTATAQLLEFLIRCAAEHPYVLHLS
                     DLARGSERTRSLVEQLARAVDHNESRILLCISTEPQPKLRPALDALRRDQVAELFNLR
                     PFQAREMLEVLQGILGDAPALKDLVVMLDKLTGGHPLSFRETLRVLIEESILVRDNDT
                     WVLRSASIAAEQLHKTLAQRSEARLDALGVSAWEIVSILYLIEAPIDEEQLAALTDLR
                     RERFRRTLDRLEGEGLILCS"
BASE COUNT         1423 a         3548 c         3641 g         1368 t
ORIGIN      
        1 cgtcgtaccg ccggtgttgg gcttggcctc actcacgggc ggcctcctgg tggcggtggg
       61 tctcggcgta caggccgttt tgcgcgagca gggactgatg cgtgccgcgc tctactactt
      121 tgccggcctc gagcacgagg atctcgtcgg cgtgctcgag caccgaggtg cggtgcgaga
      181 cgatgacggt ggtcggcggc aggccggggc ccgtcgtgcg ctcgcgggcc tggccgaccg
      241 ggatcggcgc ctcgtgcagc tggcggatcg cggcgacgag cttgagctcg gtgccctggt
      301 cgaccgcgct gagcacgtcg tcgagcagca gcagcgcggg ccggcggtag agcgcgcgcg
      361 cgagggcggt gcgctggcgc tggccgcccg agagcgtcac gccgcgctcg ccgacgacgg
      421 tgtcgaggcc ctcgggcaag gcgcggatgt cgtcgtcgag gcaggcggac gcgagcacgg
      481 tcctcagcag cgggtcgtcg gcgatcgccg cggggatcgg cttgcgcgcg ccgctgcggt
      541 cgggctcggc gtcctcgccg cgggtgtggc tgctgcgctc gccttgcagg cggatgttgt
      601 cgcgcagggt ggtcgagaac aggaaggggt cctgggggac cacagccagc ccggcgcgca
      661 ggtcggcgag cggcacgctg gtgacgtcgt ggccgtcgac gagcacgctg ccggcgggcg
      721 gcgtgtagac gcgggcgagc aggttgatga gggtgctctt gccggcgccg gtgcggccga
      781 agatgccgag ggtctgacct ggctcgaggg acacgtcgat gtcttgcagc gcgggtccgt
      841 cggcgccggg gtaggtgaag ctcagggagc gcagcgcgag ggacggcggg cgcggcaggc
      901 tgagggacgc ggtgggcacc gggaggtcgt cgggggtctg gatgacctgg tcgatgcgcg
      961 agatggcgat caggccgcgg gagatcgacg cgagcaccca ggcgagggcc atcagcaagg
     1021 cgacgaggct ggcgagcagg gtggtgaagg tggcgatcgc gccgacctgc atctcgccgt
     1081 cgatcacccg gtcgccgccg acccacagca cgagcgcggt ggcgacgagg ccggacaggc
     1141 cgagcaccgg catggcgaag gcgcgctgcg aggacagctt gagctggagg tcgaggtagg
     1201 gctggttgcg ctcgtcgaag cggcgcagca gggcctcctc ggccgcgtgg gcgcgggcgg
     1261 tgccgatgcc ggcgtagctc tcgagcacgc ggtccgacag gcgggcgagc tgctcgaagg
     1321 acacgcggat catcgcgtag aactggcgga cgacgagctg cgtataaaag gctccgagcg
     1381 cgatcggccc gaggcaccac agcgtgagca ccgggtcagt ggcccacatc tggatcaggt
     1441 gcatcgggat cgccacggcg acgttgaaca cctgcaggcc ggcgaagccg acgagcaggc
     1501 ggaccgccat ggtgtcgttg gtcgccatgc tgacgagctc gccgacccgc cgcgaggcgt
     1561 aaaacggccg ctgcatcgcc aacaagtggg tgaacaggtc gaccccgagg cgaaactcga
     1621 cgtcacgccc ggggttgaag aacagcacgc gcgacagggt ccgcacgatg accaccgcga
     1681 gcgcccagaa catcagctcg acggcgagcg agcgacccgc ggccatgccc gcgccgttgg
     1741 gtccgagcgc gtcgagtgcg ccaccgatca acgtcgggat gcggaccacg gcatagttgg
     1801 tgatgacgag catcaccgcg ccgagcgcgt actgcggcaa gttccgccgc acgtagcgct
     1861 tgaagaatcg caggacgagg ttcacagggc ggccggggcg cgaacataca gcaacggccg
     1921 ccagacccga caaggaaatc agggcgacgg atcccgcggc cgcgcgaaac caatgaaaat
     1981 gagccaggcc gggagaaccg gactggccca ggttggggtt ccttcagtgg gccgagggcc
     2041 cgccgaattc acggggtggg ggcagcagcg gtgtcacccg cggcatcgcc ggcaccgcca
     2101 gcgtcaccgg caccggcacc ggcgtcgccg gcacccgcac cctcgccgtc ggcgggggtg
     2161 gtggcgtcgg acggcggcgg cgtgtcggtc ttggtaccgt cctccggcgt ggcggtgtcg
     2221 gccttggact tgttgcagcc catggtgccg acggagagcg cagcgaacac aaacgcgatg
     2281 gtggccttct tcataaacgt tctcctgtgc aaccgttcgc cgggaaaacc cctgggaagg
     2341 ggcgtcgtct tgcgacagac gaagcgggcc aaggcagata ccgcagttcc acgacgattg
     2401 ccagaactgg tcgcatgagc tcgctgggat ggggtgaact gcgcagaggt cgccgaaaac
     2461 tggggcctcg agggcacgag gcgaggccgg ggtgggggta gacgaccact ggtttgggac
     2521 gccgttattt accctcgagg tcctcgtcat gttcgatgtc gtcgagggcc gcctcggcgg
     2581 cgtcgaggcc gtcgcgcaat tcacggatca cagcagtgaa gcgctcctgc tcggccgcgt
     2641 cgccgccgag gtcgttgtgc acgtcctccc aggcgccgta aatgcgctcg atcagcgcct
     2701 gggtgctgcc ggcgtcgacg ccctcgcgcc ggacctcgcg gtagcgcttg ctcaggtcgg
     2761 cgaacatccg gccccgggcc tcccgccagc gccggtagga ccgctgcggg tcgcgtgagc
     2821 tggcccagcg gcgcgccgaa cggtcgatgt cgtcgacgct gcggcgcagc gccttgacca
     2881 gcccgcggta ggccgcgagg tgctcgcgga catattgccg gccgccctgc tcgcgcgcgt
     2941 gggccttctc ggcggcgagg tcgtcgtcgc gaaagccggc cttgcggcgg gcctcggcca
     3001 gctcgacggg ggtcatcggc ggcggctcgg gtgggtcgct gcgggtcggc agcggcgcag
     3061 cgacggcgcg gccgggggtc ggctcgttac aggcgaggtc gacccgggaa atgaggacga
     3121 tcagggcgag agtcgcggga cgacgcatgg ctcgctccgg ctgcgccggt ggacgccggc
     3181 ggcggccctt tttacggcgg tcgccggcgc acggcaagcg gcctcggccg gacggtcggg
     3241 gctgctacac tggcggcgac gatgtcgaac ccggccgcca cgctcgaggt cttgcaggcg
     3301 gcggccgggg tgctggcggg ccacggcgga cctgcggcgc tggcgaccgt gatcgcccgc
     3361 tccggttcgg cgccgcaggt ggtcggcgcc cgtctgctgc tgcgggccga cggctcgatg
     3421 gtcggcacgg tcggcggcgg cgcgatcgaa gcccaggtgc tcgaggcgtg ccgcaccacc
     3481 ctgcgcgagg gcaccccgag cgtcgtcaag gcccacctgg tccgcgacct cggcatgtgc
     3541 tgcggcggct cgatggaggt gttcgtggag tatctcgacg cgcaggcgcg actggtgttg
     3601 atcggcggcg gacacgtcgc gcaggccctg gccccggtcg cccgcgcggt cggctttcgc
     3661 gtcgtcgtcg tcgacgaccg cgaggacatg ctcgggcacc ccgcgttcgc cgcggaccgc
     3721 cgcgagctcg ccgacgtcga cgagctcgcc gcggcggtcc cggacctcga cgagcgcgac
     3781 tacctggtga tcgcgacccg cgaccacgcg cgcgacgagc aggcgctcgc cgaattgctg
     3841 cgccggccgc accgctacat cggcatgatc ggcagccggc gcaaggtcca cgcgatcgtt
     3901 gcgaggatcg tgcggcgcga gcaggcgctc ggtcgcgggg cgccggacct cggccgcctg
     3961 cgcgcgccgg tcggcctggc gctcgggggg cggacgccgg ccgagatcgc ggtgtcgatc
     4021 gtcgccgagc tgctcgccga gcgtcacggc ggcagcgggg cgccgatgaa cgtcgtctcg
     4081 caggcgatct cccgcatcga ccgcgacctc gcggcggccc cggacgaggc ggcggagtag
     4141 cccgcggacg cggcgcggcc tgcgtcccgg gccggcctgg acctgccgca acggtcgctg
     4201 tgggcccctg gcgggtattc gtggggctct ccgggggtcc gggtggcgcg ggccgcgaca
     4261 gggccgcgtc acgtcgtcta cactcgccgc cgatgtcccg cctgctcccc gcgaagatcg
     4321 tggccgtcac ggccgtgctg ctcaccgtcc cggcctgcac ccacaagaag ccgccgccga
     4381 cggcggtcgt ggtcgacgac agctcgtcgg accagggcgg cggcacgatc tcggagccgg
     4441 ccgacggcac caggcagccg cagcggaccg gcaagccgat ccaggtgcgc gcgagcgtcg
     4501 aggggctcgg cgacctgctg gcgctggtca agcaggcgac gaccgcgtgg aacccgaaga
     4561 acccgatcga cgccaacgcg tggatccagg ccgcgctcct gcagtttggt tacgggccgg
     4621 ggatgtggaa cagcctcgac ctcaacggcg tgatggcggt cgacactgcc ttttaccccg
     4681 acatctcgac cgcggacatg cgcctgatcg gcagcgtcgc ggcgaccaac cccaagggca
     4741 tcatggaggg catgccgtcg gggcggcggc cgcagccgct cggcaatggt ctgtgggagc
     4801 tgctgcacgg cgatctacgc atggtgctgc gcgagcagcc gaaggcgctc gagtttgcga
     4861 cgacccagcc cgacctcgac cgcgccagcg cgctcggcaa gtcggctgcc ggcggccggc
     4921 gcttcaagat ccacgcgagc gatctcccgc ccggcatgct ctcggtcgat ggcgtcgctg
     4981 cgatccccgc cgggctgcgg cgtcaggtct ccgcggtgct gcgcgaggcg acccgcgcgt
     5041 ccttcgagct cgacgccggc accgaccgcg acctgaccct cgagctcgcc gccgacgcgc
     5101 ccttcgagcg cctcggcctg agcccgctcg gcgccgcgcg cacgcagacg accggcctcg
     5161 agggccgcct gccgggaggc gcggcgatcg tggtggcgat gccgtggggt tcgcccgagc
     5221 tgttgcacaa gatggtcgac aacgccgcca agcaggcgag ccagctcggc ggcgccttcg
     5281 agacccaggt ccgctcggtg atcaccgcga gccacagcgt gctcgaccag atccagaacg
     5341 acgtcgtgtt cgcggtgtac cccggcccca agggcgagat gacgatcctg atcgcggcgg
     5401 acgtcaagga cgaggcggcg gtccgcaagg cggcgcacga catcctgacg accgcgggtg
     5461 ccggggtcgc ggccttcaac gcgctcggcg gcgacaagaa ggaggcgagc ttcacggtga
     5521 cgctcaagga caagggcatc aagtccggcg cggtcgaggg cgagctgttc acgctcggcc
     5581 tgcccaagaa catggccaac gacctcgagg acgcgcgccc gctgctgacc agcaagaacc
     5641 agatcgagct cgcctcggtg gtctccggcg gcagcgcggt catcacgatc ggcttcgacg
     5701 ccaagaagct cgccggcgac gtcgccggcg gcctcaagtc cgcccgcaag accagcctcg
     5761 ccgcggacgg cggcctcgtg ctcgcccgct ccgccagcca gggctgccac ttctgcgtcg
     5821 gcatcgaccc cgtcggcttc gcccgcgtcg ccgtgttcgc cgacaagtcg atgcgcgccg
     5881 acaaggcccg caacaaggac ctcgacgccg ccgccaccgt cgtctcccgc ctcggcggcg
     5941 ccctcggctt cggcctgcgc ctcgagcccg gcaagggcgc cctcgccggc ggcctgaaga
     6001 agagcctgct cgtgctctcg cccgccgacg ccgcccaggt cggcaagctg tgggagagca
     6061 tgtcgcccgc gccggccgcc gaggacaagc cgctgtcggg cagccgcgcg aagtgaacgc
     6121 gcctggccgc gaggacatgt ccttgcggac atcccggagg cccgcgtgat cgcgacgccc
     6181 gcgccggcgg ccgtcgtgct ggcggcgggc gcgtcgacgc ggatgggccg gcccaaggcg
     6241 ctgctcgagc accacggccg cagcttcgtc gcctgcgcgg tcgacctggc ggcgcgggcc
     6301 ggctgcgcgc cgatcgtcgt cgtcaccggc gcgcaggagc tcgacggcct cggcctcgcc
     6361 gcgcggctcg tgcataacga aaattggcag cgaggccagc tgtcgagcct acagaccggc
     6421 ctcgccgcgc tcgaagaccc cgccggtgtg ctggtgctga cggtcgaccg cccgcacttg
     6481 cgagcgacga cggtcgtcac cctcgtcgcc gcgtttcgca ccgcgccgca gttctcgtgg
     6541 cagccggcgc acgcgggccg gcgcggccac ccggtgatct acccgcgaag cctgttgccg
     6601 atgctcgcgg ccctgccgac gagcgccgac ccgcgcgagc tgctgcgcgc ccacgaggcc
     6661 ctgcgccaca cgatcgccgt cgacgacccc gcggtccacg acaacctcga tcgcccggaa
     6721 gacctcgcgc gcctgccctg accatcggtt atcctcgcgg ccgatggacc tgcagcgcat
     6781 cgcgcccctc caccccgact tcgaccccgc gctcggcctg ctgcgcctcg acctcgagca
     6841 cggcaaggcc aacgagatcg gtggggtcga gctcgacgcc ttcgaggcgc tgtgcgacct
     6901 gctcgaggcc gagagcgagg tctcgtgcct gtgcacgacc agccggcggg tcagcggccg
     6961 cggcacaccg atcttcatct ccggcgccaa cgtcagcgag cgcgtcggct ggaccgacga
     7021 ccgcgtcaag ctgcacgtcg tgcgccagcg gtcgatcatg cagcgcctgc gccacgcgcc
     7081 gctgtacacg atcgtgctga cgcacggcgt caccttcggc tggggcgccg agttcgtgct
     7141 gaccgccgac tactgcctgg cgacgccgac cgcccgcttc gccctgcccg agaccggcct
     7201 ggggatcctt ccgggtgcgc gcggcaccgc cgagctcgcc tcgctggtcg gtccggcgca
     7261 ggctctgcgc ctcggctgca ccggcgagga gatcggggcc gacgaggcgc tgcgcatcgg
     7321 cctcgtgcac gagaccgtcg ccgacctcga ggccggcctc gcccgcgtcc gcgacgtcgc
     7381 ccgccgcctg cgctcgcgtt cgccgacggc cgtcgccgcg tttaagcgcg ggctgctcgg
     7441 cgccctcgga cgccccgagg ccgaacgcct cgccatcgag cgcgccgcct acgagcaatg
     7501 catcgacacc ggcgaggccg cgatcggtcg tactgcgttt agcgcgagac gcgcgagcgg
     7561 cgaggccgag gtcccgccat ggggtccgcg gcggctttag ctggccacgt ggccagagtc
     7621 gcgcggcccg gcgcgtgaac cccggatgca gatgagagat tgtccggggt ttggtgagag
     7681 cctcacaagc ttcgcgccga cgttgtcgtg gattgcgggt gaccgggtcg gcggccgggc
     7741 gcccgccgat ttttcgcgct ggcgtgggcg ggcgtgtgga atactcggga ttcacgtgca
     7801 acgcggccgg gcgcggcccg ttgtccgtgt gtgcgttcgc cgttacagtc cggccgccgt
     7861 cgctcgagcc tccatgaacc ttcccgacgc ccataacaag tccacggcgg gggacgaagc
     7921 ggagcagccg ccgatgccgg agcgcatcgc gggtcgctac cagatccgcg ggatgttggg
     7981 gcgtggcggg ttcggggcgg tctacgaggc gtacgacgag ctcgaggacc gcccggtcgc
     8041 cctcaaggtc atccggcgcg acgccagcct cgacccgccg ggctcacgct cgagcgagct
     8101 gccgtccgct accatcgcgc cggcctcgca cgcgcacagc acccggcacc tcgacagcct
     8161 gcgcaaggcc caggtgacgc gcagcttcgg caatacccaa ggcgtccctg gcgacgacat
     8221 caccctggcg ttcaaggccg agtttcgcct gctgacgcag ctgcaccacc ccaacctcgc
     8281 ggcggtctac gacttcgggc gctgcgagga cgagatggtg tacttcacgc aggaactgct
     8341 ctcggggcag agcttgcagg attacctgcg aagcgcgtca cgcgagagca tcgtcgagat
     8401 cttcgtgcag ctcgcgcgcg cgctcgacta catccacacg ctgggctttg tccacgagga
     8461 catcaagccg agcaacgtca tcgtctgcca gcccgacacc gacggcagcc agccccaggc
     8521 caagctgatc gacttcggcc tcgctcgcat ttttcgccgg caaaaggtcg ccagcaagct
     8581 cgacgacgaa ttcgcggtgc tcggcacccc cggcttctcg gccccggaga agctcaaggg
     8641 cgagccgacg gatgcacgca gcgacatcta ttcgctggcg gcgacgatct acaccgcggt
     8701 gcgtggctcg cggccgttcc cctcgcgcac cttcaaggag gcgctgagga accagatcga
     8761 ctggcggccc gagctcgcgg gtgcgctgct gcagcaggcc gggccggtgg tcgccgagct
     8821 cgtcgggcgc atgctgagcc ccgaccccaa cgtgcgcccg cagagcgccc gcagcatcgt
     8881 gctcgagctg ctgcgccgcg agtcggtgca catccgcgac cgccagaaga ccgcgcagga
     8941 ccgccgcgag ttcgcgcggg tgctcgtcga gcacttgccc ttcgtcgacc gcgccgacta
     9001 cctcgacatc ttgctggtgc gcgcctcgga ggtgctgcgt ccggagagca cgcgggtcgc
     9061 cgcgcgcggc aagccgatcc gcacgatcct catcgaggcg ccggagggca tgggcaaggc
     9121 gcgcctgatg gccgagctgc gccgcgaggt ccagctcggc gacggcgtgt tcgtcgaggg
     9181 cagctgctgg accagcgacg gcgacggcct cggcccgttc gcgccggtcg tgttgcagct
     9241 ggcgaccgcg ctcggcgagc gctcgctgac cgtcaagcag tacggcgacc tggtccgcgc
     9301 cgcccgcgag cgcagccacg agacgaccgc caccgcccag ctgctcgagt tcctgatccg
     9361 ctgcgccgcc gagcacccgt acgtcctgca cctctcggac ctcgcccgcg gctccgagcg
     9421 cacgcgctcg ctcgtcgaac agctggcccg cgccgtcgac cacaacgagt cccgcatctt
     9481 gttgtgtatc agcaccgagc cgcagcccaa gctgcgcccc gcgctcgatg cactgcggcg
     9541 cgatcaagtc gccgagctgt tcaatctccg cccgttccag gcccgcgaga tgctcgaggt
     9601 gttgcagggc atcctcggcg acgccccggc gctcaaggac ctcgtggtga tgctcgacaa
     9661 gctgaccggc ggccacccgc tgagctttcg cgagaccctg cgcgtgctga tcgaggagtc
     9721 gatcctcgtc cgcgacaacg acacctgggt gctgcgctcg gcctcgatcg ccgccgagca
     9781 attgcacaag accctcgccc agcgctccga ggcgaggctg gatgcgctcg gcgtgtccgc
     9841 gtgggagatc gtcagcatcc tctatctcat cgaggcgccg atcgacgagg agcagctcgc
     9901 cgcgctcacc gacctgcgcc gcgaacgctt ccgtcgcacc ctcgaccgcc tcgagggcga
     9961 gggcctcatc ctctgtagcg
//
LOCUS       DASWNJ010001263         4050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_405191, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001263 DASWNJ010000000
VERSION     DASWNJ010001263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405191"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VGB85_21230"
     CDS             complement(<1..642)
                     /locus_tag="VGB85_21230"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY0136626.1"
                     /translation="MPSLAHARLVRLIRLLIAVTLLVPAVARADEPLRLWHAYRDAEL
                     AALVAMIEAHGAAGGARVELLQVAHESFGSKLAAAIPLGDGPDLFIDSHERIGDYRLR
                     GLLGPADAAIDEPGAFAPNALAAVTQEGVRWGVPLSQKSVALFVNTRLVPLDRVPADL
                     EGIAELAPTLPTGVYPLAYEAQGAYGHMPLLLAFGGRMLTDEQQYAFIGPAAES"
     gene            complement(700..1341)
                     /locus_tag="VGB85_21235"
     CDS             complement(700..1341)
                     /locus_tag="VGB85_21235"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0136627.1"
                     /translation="MALTLYYGSGSPYAWKVWLALEHKQLPYELRTLSFDSRENRAPE
                     YLGLNPRGKVPTLVDGDFALWESTVILEYLEDRYPARPLLPADPRTRAVARRIAAEAD
                     AYLYPAQRALMQATLYTPEASRDPGAIAAAEAGILAELARFATYLSGDWFAGELSIAD
                     LTIYPYLRLIQRVTERMPEVTILQRFPAEITGFMRRFAALPYLEKTLPPHWRG"
     gene            1422..3491
                     /locus_tag="VGB85_21240"
     CDS             1422..3491
                     /locus_tag="VGB85_21240"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEY0136628.1"
                     /translation="MSADLHCVDLDEVLRERFGFPEFRPGQRACVEALLDGGNLLAIQ
                     PTGYGKSLLYQLPAAILEGMTVVVSPLLALVRDQLGHLNDRFDLPAASVNSDQSEEEN
                     AAARDAAAEGRIKLLFVSPEHLDHVERFAAVARLPVALLVVDEAHCISTWGHDFRPAY
                     RQIVGLVRELRAQRPALRVLALTATADARVEEDIRRQLEGPGAPPLHVHRSSMDRPNL
                     ALAVVPVNGVAHKLAYLEQLLPRLPAPGLLYCATRERTELVAEYLRLRGQDVVAYHAG
                     LEPERKRELQHEFLAGRYPAIAATNALGMGIDKSDLRFVVHVDVPGSITAYYQEVGRA
                     GRDGQPARGILLFDEDDRRIQEHFIDAAQPDPRDFEAILAAADPDDPPGLTEIKVRTG
                     LHPTRVTVVVAELVEQEFLEKRSKGGRQIYVPTGKAGGPDLERYQRQSAVRGRELDAM
                     LAYGRLGTDCLMATLRRALGDGEVAVCGRCSLCKGQRSGVHAEARVAEAEAWLERRAS
                     PIPTSMRPPMAEGLAVLDGELAGPMFRWFMKHREDGQALPQDLRELLIDRASELAGGR
                     EIAGVVVVPSRRWAGREEVTRTIAEALGVPALTSALVWREEPEHTQAELANNDQRRKN
                     VDGKLGVSGSLPRGDLLVLDDVIGSGATLREAGRALRKEGRVGGALIPIVVARVRRRL
                     GASGSGP"
     gene            complement(3543..>4050)
                     /locus_tag="VGB85_21245"
     CDS             complement(3543..>4050)
                     /locus_tag="VGB85_21245"
                     /inference="COORDINATES: protein motif:HMM:NF036518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEY0136629.1"
                     /translation="VEAMTLADLRFVLNKGQVEQSGTPLDVYNFPQTRFVAGFLGSPA
                     MNFLPATAERDEHDRHIARLADGSAVPLDPAVFPALSDGRRITLGVRPHDLSLRTGDE
                     PASLGVRAGLVELLGPELQIHGELASTPITLCLETACGVKKGDLLRLQPRSLHVFDAA
                     TGASLRAL"
BASE COUNT          557 a         1239 c         1686 g          568 t
ORIGIN      
        1 gctctcggcg gcggggccga tgaaggcgta ttgctgctcg tcggtcagca tgcggccgcc
       61 gaaggccagc agcagcggca tgtggccgta ggcgccctgg gcctcgtagg cgagcgggta
      121 gacgccggtc ggcagcgtcg gcgcgagctc ggcgatgccc tcgagatcgg cgggcacacg
      181 gtcgaggggg accagcctgg tgttgacgaa cagcgcgacc gacttctgcg acagcggcac
      241 gccccagcgc acgccctcct gggtgaccgc ggcgagggcg ttgggggcga acgcgccggg
      301 ctcgtcgatc gcggcgtcgg ccgggccgag caggccgcgc aggcggtagt cgccgatgcg
      361 ctcgtgcgag tcgatgaaca ggtcggggcc gtcaccgagc gggatcgcgg cggcgagctt
      421 gctgccgaag ctctcgtgcg cgacctgcag cagctcgacc ctggcgccgc cggccgcacc
      481 gtgcgcctcg atcatggcga cgagcgcggc gagctcggcg tcgcgatagg cgtgccacag
      541 gcgcagtggt tcgtcggcgc gggcgaccgc cggcacgagc agcgtgacgg cgatcagaag
      601 gcggatcagg cggacgagtc gcgcgtgggc gagggacggc acgaggccgc cagcatagcg
      661 aactcgcccg gtttttgccg tgtccgtggc ggccggagtt tagccgcgcc agtgcggcgg
      721 cagggtcttc tcgaggtagg gcagggcggc gaagcggcgc atgaagcccg tgatctcggc
      781 cgggaagcgc tgcaggatcg tgacctccgg catgcgctcc gtgacgcgct ggatcaggcg
      841 caggtagggg tagatcgtga ggtcggcgat cgagagctcg ccggcgaacc agtcgcccga
      901 gaggtaggtg gcgaagcggg ccagctcggc gaggatcccg gcctcggcgg cggcgatcgc
      961 cccggggtcg cggctggcct ccggggtgta gagggtggcc tgcatgaggg cgcgctgggc
     1021 ggggtagagg taggcgtcgg cctcggcggc gatccggcgg gcgacggcgc gggtccgggg
     1081 gtcggccggc aacagcgggc gcgcggggta gcggtcctcg aggtactcga ggatgacggt
     1141 cgactcccac agtgcgaagt caccgtcgac gagggtcggc accttgccgc gcgggttgag
     1201 gccgagatac tccggggcgc ggttctcgcg gctgtcgaag ctgagggtcc gcagctcgta
     1261 ggggagctgc ttgtgctcga gcgcgagcca gaccttccag gcgtagggag aaccggagcc
     1321 gtagtacagc gtgagggcca tgccacagct atacacccgc gggcgcgcga gctgacaggg
     1381 cagtcgtggt gggatcgcgc gggcggtgct atgcaggcgc gatgtctgcc gatctccatt
     1441 gcgtcgatct cgacgaggtc ctgcgcgaac gcttcggttt tccggagttt cgtccgggcc
     1501 agcgggcgtg cgtcgaggcg ctgctcgacg gcgggaatct cctggcgatc cagccgaccg
     1561 ggtacggcaa gagcctgctg tatcagctgc cggcggcgat cctcgagggg atgacggtgg
     1621 tggtgtcgcc gctgctggcg ctggtgcgcg accagctcgg gcacctcaat gaccgcttcg
     1681 atctgccggc ggcctccgtc aacagcgacc agagcgagga ggagaacgcg gccgcgcgcg
     1741 acgccgccgc cgaggggcgc atcaagctgt tgttcgtgtc gccggagcac ctcgatcacg
     1801 tcgagcgctt cgcggcggtc gctcgcctgc cggtcgcgct gctcgtcgtc gacgaggcgc
     1861 actgcatctc gacgtggggg cacgacttcc ggccggcgta tcggcaaatt gtcgggctgg
     1921 tccgcgagct gcgggcgcag cggccggcgc tgcgggtgct ggcgttgacg gcgacggccg
     1981 acgcgcgggt cgaggaggac atccgccgcc agctcgaggg gcccggcgcg ccgcccttgc
     2041 acgtgcaccg aagcagcatg gaccggccga acctcgcgct cgcggtcgtg ccggtgaacg
     2101 gggtcgcaca caagctggcg tacctcgagc agctgttgcc gaggttgccg gcgccggggc
     2161 tgctgtattg cgcgacgcgc gagcgcaccg agctggtcgc cgagtacctg cggctgcgcg
     2221 ggcaggacgt cgtcgcctat cacgcggggc tcgagccgga gcgcaagcgc gagctgcagc
     2281 acgagttcct ggcggggcgc tatccggcga tcgcggcgac gaacgcgttg ggcatgggca
     2341 tcgacaagag cgacctgcgc ttcgtcgtgc acgtcgacgt gccggggtcg atcacggcgt
     2401 attatcagga ggtcggtcgc gcgggtcgtg acgggcaacc tgcgcggggg atcctgctgt
     2461 tcgacgagga cgaccgtcgc attcaggagc attttatcga tgcggcccag ccggacccgc
     2521 gcgacttcga ggcgatcctc gcggcggccg acccggatga cccgccgggg ctgacggaga
     2581 tcaaggtgcg caccgggctg catccgacgc gggtgacggt ggtggtcgcc gagctcgtcg
     2641 agcaggagtt cttggagaaa cggtcgaagg gcgggcggca gatctatgtg ccgaccggca
     2701 aggcgggtgg gccggacctc gagcgctatc aacgacagag cgcggtgcgt gggcgggagc
     2761 tcgacgcgat gctcgcgtat ggcaggctgg gcacggactg cctgatggcg acgctgcggc
     2821 gcgcgctcgg ggacggcgag gtggcggtgt gcgggcgctg ctcgctgtgc aagggccagc
     2881 gctcgggggt gcacgcggag gccagggtcg ccgaggcgga ggcgtggctc gagcggcggg
     2941 cttcaccgat accgacgagc atgcgaccgc cgatggcgga ggggctcgcg gtgctcgacg
     3001 gggagctcgc agggccgatg ttccggtggt tcatgaagca ccgcgaggac gggcaggcgt
     3061 tgccgcagga cctgcgtgaa ttgttgatcg accgcgcgag cgagctggcg ggcgggcgcg
     3121 agatcgcggg cgtggtcgtg gtgccgtcgc ggcgctgggc cgggcgcgag gaggtcacgc
     3181 ggacgatcgc ggaggcgctc ggggtgccgg cgttgacgtc ggcgctggtg tggcgcgagg
     3241 agccggagca cacgcaggcg gagctcgcca acaacgatca gcggcgcaag aacgtcgacg
     3301 gcaagctcgg tgtctcgggg tcgttgccgc gtggggactt gttggtactc gatgacgtca
     3361 tcggctcggg cgcgacgctg cgcgaggccg gcagggccct gcgcaaggag ggccgcgtcg
     3421 gcggggcgtt gatcccgatc gtggtggccc gggtccggcg gcggctcggg gcgtccggca
     3481 gcgggccgtg aggcgcccgg tgtggggtcg gcgaacgcag gtggtcgccg gggacctgca
     3541 ggtcagagcg cgcgcaggga ggcgccggtg gccgcgtcga acacgtggag gctgcgcggc
     3601 tgcaggcgca ggaggtcgcc ttttttgacg ccgcaggcgg tctccaggca cagcgtgatc
     3661 ggggtcgagg cgagctcgcc gtggatctgc agctcggggc cgagcagctc gacgaggccg
     3721 gcgcgcacgc cgaggctggc gggctcgtca cctgtccgca gggacaggtc gtgggggcgc
     3781 acgccgaggg tgatccggcg gccgtcggag agcgcgggga acacggcggg gtcgagcggg
     3841 acggcgctgc cgtcggcgag gcgggcgatg tggcggtcgt gctcgtcgcg ctcggcggtc
     3901 gcgggcagga agttcatcgc cgggctgccg aggaagccgg cgacgaagcg ggtctgcggg
     3961 aagttgtaga cgtcgagcgg ggtgccggac tgctcgacct ggcccttgtt gagcacgaac
     4021 ctgaggtcgg cgagggtcat cgcctcgacc
//
LOCUS       DASWNJ010001264         3419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_374554, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001264 DASWNJ010000000
VERSION     DASWNJ010001264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374554"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3174)
                     /locus_tag="VGB85_21250"
     CDS             complement(<1..3174)
                     /locus_tag="VGB85_21250"
                     /inference="COORDINATES: protein motif:HMM:NF018470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein IcmF/TssM N-terminal
                     domain-containing protein"
                     /protein_id="HEY0136630.1"
                     /translation="MLKILLALLVVSLTAALWLLSDTLGLPSWAAAWATVAALFLLVG
                     MVALELLQRRSRRLRDQKRQAERSPFHQTLGNLRSRCEHALETLRAQNAEALPWVLLL
                     GPSGAGKTAALRSSGVRFLDGLGPERFITGGDVSPTESVQFLASDRLVFVDTAGRYLC
                     ETPDHEDRREWLALLNLLRTQRADRPLTAIVLTVSADALARTDPEAAASLGLGLRRRL
                     EDIQREFTATFPVYILVTRIDELAGISRLVAAELNARDPSQGEPTDRFGFEVELSGAG
                     ASSARRVAEGPLADLSLALERRAFALIEQSSTPEERGEIYRAPGHFRRLAERSADLLG
                     TLFPDHGQADAPIFRGLYFASAGASTVTPAAPADLELQALTREYGNLPQRGSGPAAPL
                     ARPAFLRGLFAQVLPEDSWIAGWTRRRQGQQRVRHGLRVALLSSAALGALSLSLGSAQ
                     SNHRLQGELSAAITAVQSDPGDPRLPLRPANLAPIQAVHNTLHAHREDGAPWSLRLGL
                     YQGAAVADEVDRVYFTTARDRLLNPTVARAQTQLTRLLAQHKDSSAALPTEQFWPAVD
                     ALHLYMLLTRPGSGSLVLQDPAQRLWLAAHLPRAWADALALTDREQAEARSVSRDYVD
                     DLAARADDLVRRDDTLVLGVREILRRTSRGQLWADELALEQIPGAGAISLSTIASAAW
                     LTTKEGRRVEAAYTRKGWDHVRNKIRCPAAYDDRYLAAALTIDDRLCPDERAALHGQY
                     FKRYVESWTQFLGDIYVREPEGYKDIESQIVDMTTPGGPGVNALENLFRVVGENAQLP
                     MVADATAAEPADFIAAVFVLARRQAAQKGIDFAGAPAAAGDQVTVATVRDAFKPYYSY
                     GYSPPGADAGAASPLSAYILLLSKVAHPLGLYTKERNQEALTQAKTSASQVYELVHNE
                     HFTQRDRRWTPALEGILDPPVRGLLDAINRGSLDELTMRWCNEVVYPFDTMRPCYPFN
                     KGATCDVSGDEVAGLFQPQSGKLWQLYADKLQERFPFQGDRYVPASQGYNSRVKLNPA
                     VARFLTNARELGEVLFPANSPAPSFSFA"
     gene            complement(3185..>3419)
                     /locus_tag="VGB85_21255"
     CDS             complement(3185..>3419)
                     /locus_tag="VGB85_21255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136631.1"
                     /translation="VAAGLVQAGKPPLLLHAPRTGDPRRPLPETRSFMWIGVSLTLFT
                     IGFYLLARYELAHMTATLLERLQSLAAAATTPA"
BASE COUNT          470 a          991 c         1481 g          477 t
ORIGIN      
        1 agcgaaggag aagctcgggg ccggtgagtt ggcggggaac aggacctcgc cgagctcgcg
       61 cgcgttggtc aggaagcgcg cgacggcggg gttgagcttg acgcgcgagt tgtaaccctg
      121 cgaggcgggg acgtagcggt caccctgaaa cgggaagcgc tcctgcagct tgtcggcgta
      181 cagctgccat agcttgccgc tctgcggctg gaataggccg gcgacctcgt cgcccgagac
      241 atcacaggtt gcgcccttat taaatgggta gcaggggcgc atggtgtcga aggggtagac
      301 gacctcgttg caccaccgca tcgtcagctc gtcgagcgag ccgcggttga tcgcgtcgag
      361 caggccgcgt acgggcgggt cgaggatgcc ctcgagcgcg ggggtccagc ggcggtcgcg
      421 ttgcgtgaag tgctcgttgt ggacgagctc gtagacctgc gaggcgctgg tcttggcctg
      481 cgtcagcgcc tcctggttgc gctccttggt gtagaggccg agcgggtggg cgaccttgga
      541 gagcagcagg atgtaggccg acagcggcga ggcggcgccg gcgtcggcac cgggcgggct
      601 gtagccgtag ctgtagtagg gcttgaaggc gtcgcgcacg gtggcgacgg tgacctggtc
      661 gccggcggca gcgggggcgc cggcgaagtc gatgcctttt tgcgcggcct ggcggcgggc
      721 gagcacgaac acggcggcga tgaagtccgc gggctcggcg gcggtggcgt cggcgaccat
      781 cggcagctgg gcgttctcgc cgacgacgcg gaacaggttc tcgagcgcgt tgacgccggg
      841 tccgccaggc gtggtcatgt cgacgatctg cgactcgatg tccttgtagc cctcgggctc
      901 gcggacgtag atgtcgccga ggaactgcgt ccacgactcg acgtagcgct tgaagtactg
      961 gccgtgcagg gcggcgcgct cgtcggggca caggcggtcg tcgatcgtga gggcggcggc
     1021 gaggtagcgg tcgtcgtagg cggcggggca gcggatcttg ttgcggacgt ggtcccagcc
     1081 cttgcgcgtg tacgcggcct cgacgcggcg accctccttg gtagtcagcc acgcggcgct
     1141 ggcgatcgtc gacagcgaga tcgcgccggc gccggggatc tgctcgagcg cgagctcgtc
     1201 ggcccacaac tggcctctgc tagtccggcg caggatctcg cggacgccga gcacgagggt
     1261 gtcgtcgcgg cgcacgaggt cgtcggcgcg ggcggcgagg tcatcgacgt agtcgcggct
     1321 gacgctgcgc gcctcggcct gctcgcggtc ggtcaaggcg agcgcgtcgg cccaggcgcg
     1381 gggcaggtgc gcggcgagcc acaggcgctg cgcggggtcc tgcaagacga ggctgcccga
     1441 gccggggcgc gtcagcagca tgtagaggtg cagggcgtcg acggcgggcc aaaactgctc
     1501 ggtcggcagg gcggcgctgc tgtccttgtg ctgggcgagc aggcgggtca gctgggtctg
     1561 ggcgcgggcg acggtcgggt tgagcaggcg gtcgcgggcg gtggtgaagt acacgcggtc
     1621 gacctcgtcg gcgacggctg ccccttgata gaggccgagg cgcagcgacc acggggcgcc
     1681 gtcttcacgg tgcgcatgca aggtgttgtg cacggcctgg atcggcgcga ggttggcggg
     1741 gcggagcggg aggcgcgggt cgccggggtc gctctgcacg gcggtgatcg ccgcgctgag
     1801 ctcgccttgc aggcggtggt tgctctgggc tgacccgagg gacaggctga gcgcgccgag
     1861 ggcggcgctc gagagcagcg cgacgcgcag gccgtgacgc acgcgctgct ggccctgacg
     1921 acgacgggtc cagccggcga tccagctgtc ctcgggcagc acctgggcga acaggccgcg
     1981 caggaaggcg gggcgcgcga gcggggctgc cgggccggag ccgcgctgcg ggaggttgcc
     2041 gtactcgcgg gtgagggctt gcaactcgag gtcggcgggg gcggcagggg tgaccgtgct
     2101 ggcgccggcg ctggcgaagt agaggccgcg gaagatcggc gcgtcggcct ggccgtgatc
     2161 ggggaacagg gtgccgagca ggtcggcgga gcgctcggcg agtcggcgga agtggccggg
     2221 cgcgcggtag atctcgccgc gctcctcggg ggtggaggac tgctcgatca gggcgaaggc
     2281 gcggcgctcg agcgcgagcg agaggtcggc gagcggaccc tcggcgacgc ggcgcgccga
     2341 gctggcgccg gcgcccgaga gctcgacctc gaagccgaag cgatccgtcg gctcgccttg
     2401 tgatgggtcg cgggcgttga gttcggcggc gacgaggcgc gagatgccgg cgagctcgtc
     2461 gatgcgggtg acgaggatgt aaacggggaa ggtcgcggtg aactcgcgct ggatgtcctc
     2521 gaggcggcgg cgcaggccga ggccgaggct ggcggcggcc tcggggtcgg tgcgcgcgag
     2581 ggcgtcggcc gagacggtga ggacgatggc ggtcagcggg cggtcggccc gctgggtgcg
     2641 gagcaagttg agcagcgcga gccactcgcg gcggtcctcg tggtcggggg tctcgcacag
     2701 gtagcggccg gcggtatcga cgaagacgag gcggtcgctg gcgaggaact ggaccgactc
     2761 ggtgggggag acgtcgccgc cggtgatgaa gcgttcgggg ccgaggccgt cgaggaagcg
     2821 gacgccgctg ctgcgcaggg cggcggtctt gccggcgccg gaggggccga gcaggaggac
     2881 ccacgggagg gcctcggcgt tttgggcgcg cagggtctcg agggcgtgct cgcagcggct
     2941 gcgcaggttg ccgagggtct ggtggaaggg tgagcgttcg gcctggcgtt tttggtcacg
     3001 cagtcggcgg ctgcggcgtt gcaggagctc gagggcgacc atgccgacga gcaggaacag
     3061 tgcggcgacg gtggcccagg cggcggccca gctcggcagg ccgagcgtgt cggacagcag
     3121 ccacagggcg gcggtcagcg agacgacgag cagcgcgagg aggatcttga gcacgaggag
     3181 gatctcaggc gggcgtggtg gcggcggcgg cgaggctctg aaggcgctcg agcagggtcg
     3241 cggtcatgtg ggcgagctcg tagcgggcga gcaggtaaaa gccgatcgtg aagagggtca
     3301 ggctgacgcc gatccacatg aaggatctgg tttcggggag gggacggcgc gggtcgccgg
     3361 tgcgcggggc gtgcagcagc agggggggct tgccggcctg gaccagcccg gcggcgacg
//
LOCUS       DASWNJ010001265         2956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_659231, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001265 DASWNJ010000000
VERSION     DASWNJ010001265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659231"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..685)
                     /locus_tag="VGB85_21260"
     CDS             complement(161..685)
                     /locus_tag="VGB85_21260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136632.1"
                     /translation="MKYLAFFSLIVSIQLSGCTEKSDDTGDEGGSSGTTGGTASTTAS
                     TTDGDPTTGTPTTTGGSETGSESDTSGTGTTVAFDEDCAFLIGKKFVSQMQFPCGPPP
                     DPNDPGPLCSDRVSFEADTFVYMSSDFGVTGTYTCEGGVIAGVDDGDGTYQGTIDAAT
                     GALVWNGNDFDVEP"
     gene            1091..2212
                     /locus_tag="VGB85_21265"
     CDS             1091..2212
                     /locus_tag="VGB85_21265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136633.1"
                     /translation="MTIPAACGAGSGEDTGTGATTGTTAPMTLSDPGATDPTTTDGLT
                     TGSPTDGTTTATTTTSTTTGDTTTTTSEPTTDATTTTTTTETTTTETTTGDTTTGNGL
                     WDSPNLWYAVDKALIYIEVDPMDGSIIKLVHNELVADTELWHGQNGLTMLEDGTLLGS
                     RESEDGTQIYHIPSPPTAENTPATAVFLGIVPGQQGEAPPRVEALYTDCDGRIYLMDT
                     GEDVGSSAGNRLLRFKGAYLKGELEFDVITDLGDASVADIDDMSPGIIDGEISDGVGF
                     AMDSSDLWRIDYTTGTGMQLAGTDGTWGIHALGGPLFTDGEPRLYILSSDAELLRVKL
                     DDYSSTSLITGPDLELDSGYNGWSGLAGPLTECMTTIPG"
     gene            complement(2230..2736)
                     /locus_tag="VGB85_21270"
     CDS             complement(2230..2736)
                     /locus_tag="VGB85_21270"
                     /inference="COORDINATES: protein motif:HMM:NF022161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 family protein"
                     /protein_id="HEY0136634.1"
                     /translation="MVRLSLRQDIDCDVETFWQHFFDPELARRLYVEGLGFPAYTTVR
                     QHETATTIERTSAIEPKLTIPAPLARVFGSSFRYTEEGVFDRATATWRWKMISGTLPD
                     KIRVTGTLRAVPVGPARVTREVEIEIEARVPLLGGLVEDTFKKQLSAGWERSAVVENQ
                     WLREVAAT"
BASE COUNT          483 a         1094 c          956 g          423 t
ORIGIN      
        1 cggcggaggc cgattcccgc cagcccgccc cgggcacgat cgatcgaccg atcggctcag
       61 ccgtcctcgg tgatcggacg aggtcgcgcg cacatacggg ccctgcgcga gcatttacgc
      121 gcacccggtc acgaggtcga gcacgcggcc ccgtgtcgcc tcacggctcg acgtcgaagt
      181 cgttcccgtt ccacacgagc gcgcccgtgg ccgcgtcgat cgtgccctgg taggtcccgt
      241 cgccgtcgtc gacgcccgcg atcacgccgc cctcgcaggt gtacgtgccg gtgacgccga
      301 agtccgagct catatacacg aacgtatccg cctcgaacga gacgcggtcg gagcacagcg
      361 ggcccggatc gttcggatcc ggcggcggcc cgcacggaaa ctgcatctgc gacacgaact
      421 tcttcccgat caggaaggcg caatcctcat cgaaggccac ggtggtcccg gtcccgctcg
      481 tatcgctctc gctgcccgtc tcgctgccgc cggtcgtcgt cggtgtaccg gtcgtcgggt
      541 cgccgtccgt ggtcgacgcc gtggtggagg ccgtgccgcc ggtcgtgccc gagctcccgc
      601 cctcgtcgcc ggtgtcgtcc gacttctcgg tgcagcccga cagttgaatg ctcacgatca
      661 gcgagaaaaa agccaagtac ttcatatgcg cacgttagca cgccgaggat cgacatacag
      721 cgccttcgga gagcaaagtc gccgggcagg tccatacatt cgccagcgct cgtcgtcgac
      781 cgcgacatgc cggtccgaac cgcgagcccg tgccattgga aactcccctc gacacgcagc
      841 cacgagcccg cgccgtgatc ctcgggtgac ggtgcgcggg ctgcccccgc gccgggtgcg
      901 gaccggcgtg ccacaccgcg cgcgctgcac cgcgcatcgc ggcagcgcgg cgcaggctgg
      961 cccccgccgc gacatcgcta gcgggcccgc ggcccggcgt gtggccggcg gcggcgtggc
     1021 ttatgctcgc cgacctggcg accccgcgat gcagcacttg gcccttaccc gcgcactcac
     1081 cgtccttttc ttgaccatcc ccgcggcttg cggcgccgga tccggcgagg acaccgggac
     1141 cggagcgacc accggcacga ccgcgcccat gacgctcagc gacccgggcg cgaccgaccc
     1201 gacgaccacc gacggcctga ccaccggctc gccgaccgac ggcacgacga ccgcgacgac
     1261 cacgacctcg acgacgaccg gcgacaccac gaccaccacg agcgagccga ccaccgacgc
     1321 caccaccacg accaccacca cggagacgac gaccaccgag accacgactg gcgacaccac
     1381 gaccggcaac ggtctgtggg actcgccgaa cctgtggtac gccgtcgaca aggcgctgat
     1441 ctacatcgag gtcgacccga tggatggatc gatcattaaa ctcgtgcata acgagctggt
     1501 cgccgacacc gagctgtggc acggccaaaa cggcctgacg atgctcgagg acggcaccct
     1561 gctcggctcg cgcgagtccg aggacgggac ccagatctat catatcccca gcccgccgac
     1621 cgcggagaac acgcccgcga cggccgtgtt cctcggcatc gtgcctggcc agcagggcga
     1681 ggcgccgccg cgggtcgagg cgttgtatac ggattgcgac gggcggatct atctcatgga
     1741 taccggtgag gatgtcggct cctcggcggg caatcgtctg ttgcgcttca agggcgcgta
     1801 tctcaagggc gagctcgagt tcgacgtgat caccgacctc ggcgacgcct cggtcgccga
     1861 tatcgacgac atgtcgcccg gcatcatcga cggcgagatc agcgacggcg tcggctttgc
     1921 gatggactcc agcgacctgt ggcggatcga ttatacgacc ggcaccggca tgcagctcgc
     1981 cggcaccgac ggcacctggg ggatccacgc gctcggcggc ccgctgttca ccgacggcga
     2041 gccgcgcctg tacatcctca gctccgacgc cgagctgctg cgcgtcaagc tcgacgacta
     2101 ctcgagcacc tccctgatca ccggccccga cctcgagctc gacagcggct acaacggctg
     2161 gagcggcctc gcgggcccgc tgacggagtg catgaccacg atccccgggt gacgccccga
     2221 gcgcgcggct caggtcgccg cgacctcgcg cagccactgg ttctcgacca ccgcgctgcg
     2281 ctcccagccg gcgctgagct gctttttaaa cgtgtcctcg acgaggccgc cgagcagcgg
     2341 gaccctggct tcgatctcga tctcgacctc acgcgtgacc cgggcagggc ccaccgggac
     2401 ggcccgcagc gtgccggtga cgcggatctt gtcgggcagg gtcccggaga tcatcttcca
     2461 gcgccacgtc gcggtcgccc ggtcgaacac gccctcctcc gtgtaacgaa acgacgatcc
     2521 gaacacgcgg gccagcggcg cggggatcgt caacttcggc tcgatcgccg aagtacgttc
     2581 gatcgtcgtc gccgtctcgt gctggcgcac ggtcgtgtac gccgggaacc ccaggccctc
     2641 gacgtacagc cggcgcgcga gctcggggtc gaagaagtgc tgccaaaacg tctccacgtc
     2701 gcagtcgatg tcctgacgca ggctgaggcg gaccatgcgc cagcgatacc acgaacccgt
     2761 gtcgcgcggg cggccggcgc gctgtgctat gggggtgagg tgatggacga cgattattac
     2821 gccccccacg cgacgaccac cctcgcgcag ccgctggcgc tgatcggcgg gtatggcagc
     2881 ggcgcgtcgc aggtcgccca ggcgctgtgc gctcgcaccg ggctgccctt cgacgacatc
     2941 gaccggacga tcgagc
//
LOCUS       DASWNJ010001266         2362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_23331, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001266 DASWNJ010000000
VERSION     DASWNJ010001266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23331"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1470
                     /locus_tag="VGB85_21275"
     CDS             <1..1470
                     /locus_tag="VGB85_21275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136635.1"
                     /translation="HIFNVDSSLRAPAEAFMARHGIERLETGRDWERWSEAEMQEQLR
                     HVLARELVPEVTTYRSPLTAVDAVRVVGLVAGSLAMLLLMLFAPVLAGTQMAQEVHEN
                     TLQPLTGTALSARQLVLGMTLGPAAVIALLAAPQLLLFLAAAVAFGHTLPALGMVAVC
                     LASGTFLTMLAQLAGLALGRQRSSGMLGVTLLALLAPLTMLGAALALELPSRAVGVLA
                     LLPQAAASHMLLESFLPAGTLMRCPNLDGAELASAQWAVVIGALGMLCFGWLGMRALE
                     RRVGELSPTALSRGEALLGAVVSIVLITLANPYAPQSYQAGPDFYLLNFGMILVPLSI
                     LLMMRVPMSETPTVLRRVPIASLLLELTAGVALFFAVCAGCMGGVQHLGVFASPVALA
                     YLVWTIAVAGLLTLRVTALPMGMLAKLWVGVCSVGLGVALVHTIEWTREPGQEIAAAL
                     GFWQVSPLLGAVQAIMLVLIPALLLRALRHPASTTPAEG"
     gene            1539..>2362
                     /locus_tag="VGB85_21280"
     CDS             1539..>2362
                     /locus_tag="VGB85_21280"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0136636.1"
                     /translation="MTDSPLLISSLIRHAARVFGDQEIVSRSVEGPIHRTNYAEIHAR
                     SQRLANALQALGVGPGDRVATLAWNTHRHVEIYFGVSGLGAICHTLNPRLFPEQLLYM
                     LRHAGDVLLFVDLTFLPLVAKLAPQCPELRGVVALCERGCLPADAPKELLVYEELLAA
                     QSPSFAWPTLDENSASSLCYTSGTTGEPKGVLYSHRSTLLHSFAIALPDLFNLGEHET
                     VLPVVPMFHVNAWGIPYAAAMTGTRLVMPGPKLDGESVYQLLESERVTMTAGVPTV"
BASE COUNT          269 a          962 c          744 g          387 t
ORIGIN      
        1 cacatcttca acgtcgactc cagcctgcgc gcccccgccg aggccttcat ggctcgccac
       61 ggcatcgagc gcctcgagac cggacgcgac tgggagcgct ggtccgaggc cgagatgcag
      121 gagcagctgc gccacgtcct cgcgcgtgag ctcgtccccg aggtcaccac ctaccgctcc
      181 ccgctgaccg ccgtcgacgc cgtccgcgtc gtcggcctcg tcgccggcag cctcgccatg
      241 ctcctcctca tgctgttcgc gccggtcctc gccggcacgc agatggccca ggaggtccac
      301 gagaacaccc tccagccgct gaccggcacc gccctgtccg cccgccagct cgtgctcggc
      361 atgaccctcg gcccggccgc cgtcatcgcc ttgctcgccg cgccgcagct gctgctgttc
      421 ctcgccgccg ccgtcgcctt cggacacacc ctgcccgccc tcggcatggt cgccgtgtgc
      481 ctcgccagcg gcaccttcct gaccatgctc gcgcagctcg ccggcctcgc gctcggccgc
      541 cagcgcagca gcggcatgct cggcgtcacc ctgctcgccc tgctcgcccc gctgacgatg
      601 ctgggcgccg ccctcgcgct cgagctgccg agccgcgccg tcggcgtgct cgccctgctc
      661 ccgcaggccg ccgcctcgca catgttgctc gagagcttcc tgcccgcggg caccctgatg
      721 cgctgcccga acctcgacgg cgccgagctg gcgtcggcgc agtgggccgt cgtcatcggc
      781 gcgctcggca tgctgtgctt cggctggctc ggcatgcgcg cgctcgagcg ccgggtcggc
      841 gagctgtccc cgaccgcgct gtcgcgcggc gaggcgctgc tcggcgcggt cgtcagcatc
      901 gtgctgatca cgctcgccaa cccctacgcc ccgcagagct accaggcggg ccccgacttc
      961 tacctcctca acttcggcat gatcctcgtg ccgctgtcga tcctgctgat gatgcgcgtg
     1021 ccgatgtccg agaccccgac ggtcctgcgc cgcgtcccga tcgcgtcctt gctgctcgag
     1081 ctcaccgccg gcgtcgcgct gttcttcgcg gtgtgcgcgg gctgcatggg cggcgtccag
     1141 cacctcggcg tcttcgccag cccggtcgcc ctcgcctacc tcgtgtggac gatcgccgtc
     1201 gccggcctgc tgaccctccg cgtcaccgcg ctgccgatgg gcatgctcgc caagctgtgg
     1261 gtcggcgtgt gctcggtcgg cctcggcgtc gcgctcgtcc acacgatcga gtggacccgc
     1321 gagcccgggc aggagatcgc cgccgcgctc ggcttctggc aggtcagccc gctgctcggc
     1381 gccgtgcagg cgatcatgct ggtcctgatc cccgccctgc tgctgcgggc cctgcgtcac
     1441 ccggcctcca ccacgcccgc ggagggctga gcgtcggcca ccgccaggcg gttgacggcc
     1501 gcgcgcgagg cccctagcgt ggtctcatgc gcggcctgat gacggactct cccctgctga
     1561 tctcctcgct gatccggcac gccgcgcgcg tgttcgggga ccaggagatc gtcagccgca
     1621 gcgtcgaggg gccgatccac cgcaccaact acgcggagat ccacgcccgc tcccagcgcc
     1681 tcgccaatgc actgcaggcg ctcggcgtcg gccccggcga ccgcgtcgcc acgctcgcgt
     1741 ggaacaccca ccgccacgtc gagatctact tcggcgtgtc cggcctcggc gcgatctgcc
     1801 acaccctcaa cccgcgcctg ttccccgagc agctgctcta catgctgcgc cacgctggcg
     1861 acgtgctgct gttcgtcgac ctgaccttct tgcccttggt cgcaaagctc gcgccgcagt
     1921 gccccgagct gcgcggcgtc gtcgcgttgt gcgagcgcgg ctgcctgccc gcggacgccc
     1981 cgaaggagct gctcgtatac gaggagctgc tcgccgccca gtcgccgagc ttcgcctggc
     2041 cgaccctcga cgagaacagc gcctcctcgc tctgctacac ctccggcacc acgggcgagc
     2101 ccaagggcgt gctctactcg catcgctcga ccctgctgca ctcctttgcg atcgcgctgc
     2161 ccgacctgtt caacctcggc gagcacgaga ccgtgctgcc cgtggtcccg atgttccatg
     2221 tcaatgcatg ggggatcccc tacgccgcgg cgatgaccgg cacgcggctc gtcatgcccg
     2281 gccccaagct cgacggcgag agcgtgtacc agttgctcga gtcggagcgc gtcacgatga
     2341 ccgccggcgt gccgaccgtg tg
//
LOCUS       DASWNJ010001267         2611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_92998, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001267 DASWNJ010000000
VERSION     DASWNJ010001267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92998"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VGB85_21285"
     CDS             <1..253
                     /locus_tag="VGB85_21285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136637.1"
                     /translation="LRRGDVVREEHAGSDGTFAFRGVTPGECTLQLLVGGVTQTTALV
                     FASGERRVLDLVVDPTKRPKVDPALREKARREGDMVELR"
     gene            complement(246..1322)
                     /locus_tag="VGB85_21290"
     CDS             complement(246..1322)
                     /locus_tag="VGB85_21290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136638.1"
                     /translation="MQMLQMDIARRLDEIASMTTDDARSAWNRCRYDPARGAGHYESY
                     FQRANHPTRPLGFWIRYTIFSPEDHPRDAVGELWAIWFDGERGKVTAVKSVVPVGHCR
                     FDRTCLSAQVGEARLNEARLAGAAASTGHAIAWDLEYSGDDAPVLLLPENLYEGPFPN
                     AKALVGTPNARYHGTISVDGEVHAIDRWVGSQNHNWGSRHTDRYAWAQVAGFDGVPDS
                     FLECSTAQIKIGPLWSPRFTLVVLRIEGEEYAINSLPRAMIADGRWAAVESGFEWHLR
                     TKQAGVEIAAKISAPRERFIGLRYQNPPGGTKTCLNTKLARVELTLRRAGRPELHLRS
                     EHRAAFEMLTDDSVAGVPGVPIIT"
     gene            complement(1335..>2611)
                     /locus_tag="VGB85_21295"
     CDS             complement(1335..>2611)
                     /locus_tag="VGB85_21295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136639.1"
                     /translation="VCAAPVRILGQVVDAVTLAPLPDALVNGLDRTGAPLGEIAVTDA
                     TGHYELLVSAPRTIDGELSPGAAYTLQGFAADYQPFPSGIRVALPISAADATYDDGLG
                     SFVIDNPSTVVGLIGLPMAERGGVTISGKVLGSEPGGTLVVAEGQTPASYGVADSTGA
                     YTVFNVRDGAATLHGYRRGLEVVPRDVTVAAADLTGIDLDVAAEGEGLAVVSGSVNIV
                     NAPGGSKTSVVLVPSSVFNTNLLRGPVPFGLRAPDPGIAPEVSGAFRITGVPAGTYKV
                     LAAFENDGLVRDPDLTIGGTSLQEITVEAGRDTPLAESFKVTEALAVTSPGAEAPEVV
                     SGTPTFVFADDSSEDYYIVRVFDVFGELVWEDAMVPGVSGSATVEVKYAGPPLTPGMY
                     YQFRATSAREKGGGSAISQTEDLRGVFIAGGQ"
BASE COUNT          370 a          946 c          915 g          380 t
ORIGIN      
        1 cctgcgccgc ggtgatgtcg tacgcgagga gcacgccggc tccgacggca ccttcgcgtt
       61 tcgcggcgtc acgcccggcg agtgcacgct ccagctgctc gtcggcggcg tcacgcagac
      121 caccgcgctg gtgttcgcgt ccggcgagcg ccgcgtcctc gacctcgtcg tcgaccccac
      181 gaagcggccg aaggtcgacc ccgccctgcg cgagaaagca cggcgcgagg gcgatatggt
      241 cgagctcagg tgatgatcgg cacacccggc acgccggcga cgctgtcgtc ggtcagcatc
      301 tcgaacgcgg ctcgatgctc gctgcgcagg tgcagctccg ggcggccggc gcggcgcagg
      361 gtcagctcga cgcgcgccag cttggtgttg aggcaggtct tggtgccacc gggcgggttc
      421 tgatagcgca ggccgatgaa gcgctcgcgc ggcgccgaga tcttggccgc gatctcgacc
      481 ccggcctgtt tggtccgcag gtgccactcg aagccggact cgaccgcagc ccagcggccg
      541 tcagcgatca tggcgcgcgg cagcgagttg atcgcgtact cctcgccctc gatgcgcagc
      601 accaccaggg tgaagcgcgg cgaccacagc gggccgatct tgatctgcgc ggtcgagcac
      661 tccaaaaagc tgtccggcac cccgtcgaag ccggcgacct gggcccaggc gtagcggtcg
      721 gtgtggcggc tgccccagtt gtggttctgg ctgccgaccc accggtcgat cgcgtgcacc
      781 tcaccgtcga ccgagatcgt cccgtggtac ctcgcgttcg gcgtcccgac gagcgccttg
      841 gcgttcggga acgggccctc gtacaaattc tcgggcagca gcagcaccgg cgcgtcgtcg
      901 ccgctgtact cgaggtccca cgcgatcgcg tggccggtgc tcgccgcggc gccggcgagc
      961 cgggcctcgt tgaggcgggc ctcgccgacc tgcgccgaga gacaggtgcg gtcgaagcga
     1021 cagtgcccga ccggcaccac cgacttcacc gccgtcacct tgccgcgctc accgtcgaac
     1081 cagatcgccc acagctcgcc gaccgcgtcg cgtgggtggt cctccgggct gaagatcgtg
     1141 tagcggatcc aaaacccgag cgggcgcgtg gggtggttcg cgcgttgaaa gtaactctcg
     1201 tagtgccccg ccccgcgggc ggggtcgtac cggcaccggt tccaggcgga ccgcgcgtcg
     1261 tccgtggtca tgctggcaat ctcgtccagc cggcgcgcga tgtccatctg caacatttgc
     1321 atggggtcga tccctcactg gccgccggcg atgaacactc cgcgaaggtc ctcggtctgc
     1381 gagatcgccg agcccccacc cttctcgcgg gccgaggtcg cccggaattg ataatacatt
     1441 ccgggcgtca acgggggccc ggcgtacttc acctcgaccg tggcgctgcc cgacaccccg
     1501 gggaccatcg cgtcctccca gaccagctcg ccgaacacgt cgaacacgcg aacgatgtaa
     1561 tagtcctcgc tcgagtcgtc cgcgaacaca aaagtcggcg tcccgctgac gacctcgggg
     1621 gcctcggcac ccgggctcgt gaccgccagc gcctccgtga ccttgaagct ctccgcgagc
     1681 ggggtgtcgc ggccggcctc gacggtgatc tcctgcaggc tggtgccgcc gatcgtcagg
     1741 tcggggtcgc ggaccaggcc gtcgttctcg aaggccgcca gcaccttgta cgtgcccgcc
     1801 ggcacgcccg tgatccggaa cgccccgctg acctccggcg cgatgccggg gtcgggcgcc
     1861 cgcaggccaa agggcaccgg accgcgcagc aggttggtgt tgaacacgct cgacggcacg
     1921 agcaccacgc tcgtcttgct gccgccgggc gcgttgacga tgttgaccga gccgctgacg
     1981 accgccagac cctcgccctc ggcggcgacg tcgaggtcga tgcccgtcag gtcggcggcg
     2041 gcgaccgtga catcgcgggg gacgacctcg aggccgcggc ggtagccgtg cagcgtcgcc
     2101 gccccgtcgc gcacgttgaa cacggtgtac gccccggtgc tgtcggccac gccgtagctc
     2161 gccggcgtct ggccctcggc gaccaccagc gtgccgccag gctcgctgcc gaggaccttg
     2221 ccggagatcg tgacgccgcc gcgctcggcc atcggcaggc cgatcaggcc gacgacggtc
     2281 gacgggttgt cgatcacgaa cgacccgagg ccgtcgtcgt aggtcgcgtc cgcggcggag
     2341 atcggcagcg cgacgcggat gccggacggg aacggctgat agtcggcggc gaagccctgc
     2401 agcgtgtacg cggccccggg cgagagctcg ccgtcgatcg tccgcggcgc gctgaccagc
     2461 agctcgtagt gacccgtcgc gtcggtgacc gcgatctcgc cgagcggcgc gccggtgcgg
     2521 tcgaggccgt tgacgagcgc gtccggcagc ggcgcgagcg tgacggcgtc gaccacctga
     2581 ccgaggatcc gcaccggagc ggcgcacacg t
//
LOCUS       DASWNJ010001268         1970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_93015, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001268 DASWNJ010000000
VERSION     DASWNJ010001268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93015"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /gene="greA"
                     /locus_tag="VGB85_21300"
     CDS             complement(<1..246)
                     /gene="greA"
                     /locus_tag="VGB85_21300"
                     /inference="COORDINATES: protein motif:HMM:NF015414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY0136640.1"
                     /translation="MSTNRIPMTPEGHTRLSAELKRLKNIERPAVIKAIAAAREHGDL
                     SENAEYHAARERQGFIEGRVMELEDKLSRAEVIDLNSL"
     gene            complement(319..>1970)
                     /gene="carB"
                     /locus_tag="VGB85_21305"
     CDS             complement(319..>1970)
                     /gene="carB"
                     /locus_tag="VGB85_21305"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY0136641.1"
                     /translation="PYYYSTYETPYTVNGKTVVEDEIRLTDRPKVIIIGGGPNRIGQG
                     IEFDYCCVHAAFGLKEAGIETVMVNCNPETVSTDPETSDRLYFEPLTAEDVLEICRVE
                     ASRGELMGVIVQLGGQTPLKLARHLEAAGVPVLGTSPDAIDLAEDRERFQGLLHRLGL
                     KQPENDIAMDAAEAVAKAAGLGFPLVIRPSYVLGGRAMRIAHLPEEVHAFAVEAARAS
                     ESGPVLLDRYLADAIEVDVDVLADQRTVRVVGVMEHVEEAGVHSGDSACSLPPYTLAA
                     EVVAEIERQAVALAEALGVRGLMNAQMAVKDGEVYILEVNPRASRTVPFVAKAIGYPV
                     AKVAARIMAGERLADFPFRDRSSSGGHVAVKEAVFPFARFPGVDLLLGPEMKSTGEVM
                     GLDRDFAAAFVKSQIAAGNELPRSGSVFVSVRDRDKPVVVGLARTLCDLGFTLLATQG
                     TAAALREAGLPVRDVLKVHEGRPHIVDLIKDRGVALVINTTEGRQAVRDSYSLRRAAL
                     TARLPYYTTLPGARAVVQALDRLISGELEVAPLQAYFDTPH"
BASE COUNT          268 a          724 c          683 g          295 t
ORIGIN      
        1 caggctgttg aggtcgatga cctcggcccg gctgagcttg tcctcgagct ccatcacccg
       61 gccctcgatg aacccctggc gctcgcgcgc ggcgtggtac tcggcgttct ccgagaggtc
      121 gccgtgctcg cgcgccgccg cgatcgcctt gatgacggcc gggcgctcga tgttcttgag
      181 ccgcttgagc tcggcgctca gccgggtgtg gccttccggc gtcatcggga tcctgttcgt
      241 cgacatcgcg agacgagttc cgtgctgggt gcccaaaacg agatcgcgcc gcgcgtccgc
      301 ttcccgacgc cggcgcgatc aatgtggggt gtcaaaatac gcttgcagcg gcgctacttc
      361 aagctcgccg ctgatcagcc ggtccagggc ctgcaccacg gcccgcgcgc cgggcagcgt
      421 cgtgtagtag ggcagcctcg cggtgagggc cgcgcgccgc agcgagtagc tgtcgcggac
      481 cgcctggcgg ccctcggtgg tgttgatgac caacgctacg ccgcggtcct tgatcaggtc
      541 gacgatgtgc ggccggccct cgtggacctt cagcacgtcg cgcaccggca gcccggcctc
      601 gcgcagcgcc gcggccgtgc cctgcgtcgc caggagggtg aagccgaggt cgcacaaggt
      661 tcgcgccagc cctaccacca ccggcttgtc ccggtcgcgc acggagacga acaccgagcc
      721 cgagcgcggc agctcgttgc cggccgcgat ctgcgacttc acgaacgccg ccgcgaagtc
      781 gcggtccagg cccatgacct cgccggtcga cttcatctcc ggcccgagca gcaggtcgac
      841 gccggggaag cgcgcgaagg ggaacaccgc ctccttcacc gccacgtgcc cgcccgacga
      901 ggaccggtcg cggaagggga agtcggccag ccgctcgccc gccatgatcc gggccgcgac
      961 cttggccacc gggtagccga tcgccttggc cacgaacggc acggtccggc tggcgcgcgg
     1021 gttgacctcc aggatgtaga cctcgccgtc cttgacggcc atctgcgcgt tcatcagccc
     1081 gcgcacgccc agcgcctcgg ccagcgccac cgcctgccgc tcgatctcgg ccaccacctc
     1141 ggcggccaag gtgtagggcg gcagcgagca ggcgctgtcg cccgagtgca ccccggcctc
     1201 ctcgacgtgc tccatgacgc ccaccacccg cacggtgcgc tggtcggcca gcacgtcgac
     1261 gtcgacctcg atcgcgtcgg cgaggtagcg gtcgagcagc accggccccg actccgacgc
     1321 gcgcgcggcc tcgaccgcga aggcgtggac ctcctcgggc aggtgcgcga tccgcatcgc
     1381 ccgcccgccc agcacgtagg acgggcggat caccagcggg aagcccagcc cggccgcctt
     1441 cgcgaccgcc tcggccgcgt ccatcgcgat gtcgttctcg ggctgcttca ggccgagccg
     1501 gtgcagcagc ccctggaagc gctcgcggtc ctcggccagg tcgatggcgt cgggcgaggt
     1561 gcccagcacg ggcacgccgg cggcctccag gtggcgggcc agcttgagcg gcgtctggcc
     1621 gccgagctgg acgatgacgc ccatcagctc gccgcgggag gcctcgacgc ggcagatctc
     1681 gagcacgtcc tcggcggtga gcggctcgaa gtagagccgg tcggaggtct cggggtcggt
     1741 cgagacggtc tccgggttgc agttgaccat caccgtctcg atcccggcct ccttcaggcc
     1801 gaaggcggcg tggacgcagc agtagtcgaa ctcgatcccc tggccgatcc ggttcgggcc
     1861 gccgccgatg atgatcacct tcgggcggtc ggtcagccgg atctcgtcct cgacgaccgt
     1921 cttgccgttc acggtgtagg gcgtctcgta cgtcgagtag tagtagggcg
//
LOCUS       DASWNJ010001269         2505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_200171, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001269 DASWNJ010000000
VERSION     DASWNJ010001269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200171"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VGB85_21310"
     CDS             complement(<1..264)
                     /locus_tag="VGB85_21310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136642.1"
                     /translation="MHARVSWVLFALLACSNRTSTPEPGVPVSEPAQPERAVPTPLRV
                     TPVPAGQGVEGVVRIESGDHRFRGVLLERTDGERLVLAYRPLEW"
     gene            complement(423..1139)
                     /locus_tag="VGB85_21315"
     CDS             complement(423..1139)
                     /locus_tag="VGB85_21315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136643.1"
                     /translation="MRTMFIGVCLASLAACGDSGATSTTSGSTSEAAPSTTGATTGEP
                     TTGTGATTGTTTGTTTGETTTGQTATGDTTSSPSPTSSTTGDASTGTASTTEPDDTTT
                     TDPEDTTGGATSPLVIAVVDAYLVADCTQPTPDPVQGGWYVEFDNVNNLAETSVILKS
                     ASLSLLDADPPVLEPITVQPTESGPLPAGEYVSQEMIKLPGAAHSACDHCGEFYMLVL
                     EYDEAGYIHHVDEEMTIQCD"
     gene            1236..1817
                     /locus_tag="VGB85_21320"
     CDS             1236..1817
                     /locus_tag="VGB85_21320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136644.1"
                     /translation="MRSCVGVTVLAMSITSDAAASPWALRRMQPEAVGEPAPAAVPGA
                     AAPVQPGPPPPIAGGPGSTDVPGTRPGAVEQRPIIIHQTPPPGGSGLIAGGIVVTILG
                     AAIVGGGAALIVRNRPRDDDGLTEEVVGAYANVGGIILIVLGVAPVLGGVVMTSVGVG
                     RERDLQNWQQKQLSPGVWRTPHGSWTLGMKLAF"
     gene            complement(1814..>2505)
                     /locus_tag="VGB85_21325"
     CDS             complement(1814..>2505)
                     /locus_tag="VGB85_21325"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0136645.1"
                     /translation="RRVYQTVGKDLELGQPDAFPALLAVLGNQRSSMGVALTEILTGM
                     NIGFEAVSEDFATYFADDPDARIYWELSRARIAYAGAIALADAYMAAREKVVRAQADE
                     ILRLSTQVLPLYRGIVLFPLVGTIDATRAQAILTVLLGVVSKYSARVVLIDISGVPVV
                     DSEAARHLCRTAQAVGLLGAAAVLVGTSADVARTMVTAGIDLGRMETLSDLESGLQHA
                     LALLGRKIVAR"
BASE COUNT          325 a          811 c          996 g          373 t
ORIGIN      
        1 ccactcgagc ggccggtacg cgagcacgag gcgctcgccg tcggtgcgct cgagcaagac
       61 gccgcgaaac cgatggtcgc ccgactcgat ccgcacgacg ccctcgacgc cctggccggc
      121 cggcaccggg gtgacccgca gcggcgtggg caccgctcgc tccggctgcg ccggctcgct
      181 cactggcacc cccggctcgg gggtcgaggt gcggttgctg caggcgagca gcgcgaacaa
      241 gacccaggag acccgagcgt gcatggcggg agagtatcag cgcaggacaa catgcggcga
      301 ggtgctcgcg tgggccgatc gcgcggcgcg agccaccacg cgcgcgggca ctcgccgagt
      361 cgcgcgcgtg gcccgcgagg gcgtctcgca agctcgaccg tggtgtgtcg gccgcgcccg
      421 cgtcagtcac actggatcgt catctcctcg tcgacgtggt ggatgtagcc ggcctcgtcg
      481 tactcgagca cgagcatgta gaactcgccg caatgatcgc aggcgctgtg ggcggcgccg
      541 gggagcttga tcatctcctg gctgacgtac tcgccggcgg gcagcgggcc gctctcggtc
      601 ggctgcaccg tgatcggctc gagcaccgga ggatcggcgt cgagcagcga gagcgaggcc
      661 gacttgagga tcacggaggt ctcggcgagg ttattgacgt tgtcgaactc gacgtaccag
      721 ccgccctgca ccgggtcggg ggtcggctgc gtgcagtcgg cgacgaggta tgcatcgacg
      781 acggcgatca ccagcggact cgtcgcgccg ccggtcgtgt cctcggggtc agtggtcgtg
      841 gtgtcgtcgg gctcggtggt gctcgcggtg ccggtgctcg cgtcgccggt ggtcgatgat
      901 gtcggtgatg gtgatgaggt cgtgtcgccg gtcgcggtct ggcccgtggt cgtctcgccg
      961 gtggtcgtgc ccgtggtcgt gcccgtggtc gcgcccgtcc cggtggtcgg ctcgccggtg
     1021 gtcgcgccgg tggtcgacgg ggccgcctcg ctggtgctgc cgctcgtggt ggaggtcgcg
     1081 ccgctgtcgc cgcaggcggc gagggaggcc agacagacgc cgatgaacat tgttcgcatg
     1141 ggcggcgtgt agcccgggcc tcgcgggcct gcaagcgggg gtctgcccgc ggggtcgtct
     1201 gttcagcgtg agggccggtc gtttatgctg gggtcatgcg gtcgtgcgtg ggcgtcacgg
     1261 tgctggcgat gtcgatcacg agcgatgcag cggcgagccc gtgggcgctg cgacggatgc
     1321 agccggaggc ggtcggtgag cctgcgcccg ctgcggtgcc cggggcggcg gcgccggtgc
     1381 agcccgggcc gccgccgccg atcgcgggtg gaccggggtc gaccgacgtg cctggcacgc
     1441 ggcccggggc ggtcgagcag cggccgatca tcattcacca gaccccgccg ccgggcggct
     1501 cggggctgat cgccggcggg atcgtcgtga cgatcctggg ggccgcgatc gtcggcggcg
     1561 gggcggcgct gatcgtccgg aatcggccgc gggacgacga tggcctgacc gaggaggtcg
     1621 tcggcgccta cgcgaacgtc ggcgggatca tcctgatcgt gctcggcgtg gcgccggttc
     1681 tcggcggggt cgtgatgacc tcggtcggcg tcgggcgcga gcgtgacctg caaaactggc
     1741 agcaaaagca gctctcgccg ggcgtctggc gcacgccgca cggcagctgg acgctcggca
     1801 tgaagctggc gttctagcgg gcgacgatct tgcgaccgag cagcgcgagc gcgtgttgca
     1861 ggccgctctc gaggtccgag agggtctcca tgcggccgag gtcgatgccg gcggtgacca
     1921 tcgtgcgggc gacgtcggcg ctggtgccga cgaggaccgc ggcggcgccg agcaggccga
     1981 ccgcctgggc ggtgcgacac aggtgccggg cggcctcgct gtcgacgacg ggcacgcccg
     2041 agatgtcgat cagcacgacg cgcgcgctgt acttgctgac gacgccgagc agcacggtca
     2101 agatcgcctg ggcccgcgtc gcgtcgatcg tgccgacgag cgggaacagg acgatgccgc
     2161 gatacagcgg caacacctgg gtcgagaggc gcaggatctc gtcggcctgg gcgcggacca
     2221 ccttctcgcg ggcggccatg taagcgtcgg ccagcgcgat cgcgccggcg taggcgatgc
     2281 gggcccgcga cagctcccag tagatccggg cgtcggggtc gtcggcgaag tacgtagcga
     2341 agtcctccga gaccgcctcg aagccgatgt tcatgccggt caagatctcg gtcagcgcga
     2401 cgcccatcga ggagcgctgg ttcccgagca cggcgagcag cgcggggaaa gcgtcgggtt
     2461 ggccgagctc gaggtccttg ccgacggtct ggtacacgcg gcgga
//
LOCUS       DASWNJ010001270         1677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_619930, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001270 DASWNJ010000000
VERSION     DASWNJ010001270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619930"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VGB85_21330"
     CDS             <1..805
                     /locus_tag="VGB85_21330"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY0136646.1"
                     /translation="AAFPDPTLVKWNSFDGKEISGFAYRPDPAKFPGKRPIVINIHGG
                     PESQYRPGFMGAYNYYFTRLGVAAVFPNVRGSSGYGKTYLTLDDGRKREDSVKDIGAL
                     LDWIATQPELDADRVMITGGSYGGYMTLAAAVQYADRIRCAIDIVGISNFVTFLEHTE
                     AYRRDLRRVEYGDERDPQMRAFLQKISPLTQADRITKPLFVIQGRNDPRVPASEAEQI
                     VATLKQRGTPVWYLEGKDEGHGFQKKRNQDYQMYATIQFMKTYLLDGMP"
     gene            complement(823..>1677)
                     /locus_tag="VGB85_21335"
     CDS             complement(823..>1677)
                     /locus_tag="VGB85_21335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136647.1"
                     /translation="LAAVGLLVSIPSVAQASLDACGGVYLSVDAQCELVPTETCTTKC
                     EEVAMEVSCAAQLYADCEIGCTATAEVTCIEDCGPNCAPTCETQRATDDPPNARGLCM
                     SSCQQDCNNDCAGSERMGECRSSCAHTCGDLCEKNCDTEPSTVCAPTCEVACQGSCRA
                     QASSSCVVTCEAESFATCKTELVTVCNEDCKTTGGVLFCDAQFLNATDLQACADQLYA
                     EFAIEVDVEINVECKDGMCEADSKGAVEAEGEGGCLSTIDTRGGLGGLLMAMGIVGLG
                     LIRRRRAA"
BASE COUNT          272 a          580 c          578 g          247 t
ORIGIN      
        1 ggccgcgttc cccgacccga cgctggtgaa gtggaacagc ttcgacggga aggagatcag
       61 cgggttcgcg taccgcccgg acccggccaa gttcccgggc aagcggccga tcgtgatcaa
      121 catccacggc ggcccggagt cgcagtaccg gcccgggttc atgggcgcgt acaactacta
      181 cttcacccgg ctcggggtgg cggccgtgtt cccgaacgtc cgcgggtcga gcgggtacgg
      241 caagacgtac ctgacgctcg acgacgggcg caagcgcgag gactcggtca aggacatcgg
      301 cgcgctgctc gactggatcg cgacgcagcc ggagctcgac gccgaccggg tgatgatcac
      361 cggcggctcg tacggcggct acatgacgct cgccgcggcg gtgcagtacg ccgaccggat
      421 ccgctgcgcg atcgacatcg tcgggatctc caacttcgtc accttcctcg agcacaccga
      481 ggcgtaccgc cgcgacctgc gtcgcgtcga gtacggcgac gagcgcgacc cgcagatgcg
      541 cgccttcttg cagaagatct cgccgctgac gcaggccgat cggatcacaa agccgctgtt
      601 cgtcattcag ggccgcaacg acccgcgcgt gcccgccagc gaggccgagc agatcgtcgc
      661 cacgctcaag cagcgcggca cgcccgtctg gtacctcgag ggcaaggacg agggccacgg
      721 cttccagaag aagcgcaacc aggactatca gatgtacgcg acgatccagt tcatgaagac
      781 ctacctgctc gacggcatgc cgtgagcagg taggccgcgt gatcacgcgg cgcgacgacg
      841 acggatcagg ccgaggccga cgatacccat cgccatcagc aggccgccga gcccgccgcg
      901 ggtgtcgatc gtcgacaggc agccgccctc gccctcggcc tcgacggcgc ccttgctgtc
      961 cgcctcgcac atgccgtcct tgcactcgac gttgatctcg acgtcgacct cgatcgcgaa
     1021 ctcggcgtac agctggtcgg cgcaggcctg gaggtcggtc gcgttgagga actgggcgtc
     1081 gcagaacagc acgccgccgg tcgtcttgca gtcctcgttg cacaccgtga ccagctcggt
     1141 cttgcaggtc gcgaacgact ccgcctcgca ggtcacgacg cagctgctgc tcgcctgggc
     1201 ccggcaggag ccctggcacg cgacctcgca ggtcggcgcg cacacggtcg agggctcggt
     1261 gtcgcagttc ttctcgcaca ggtcgccgca ggtgtgcgcg caggacgagc ggcactcgcc
     1321 catccgctcc gagccggcgc agtcgttgtt acagtcctgc tggcagctcg acatgcacag
     1381 gccgcgggcg ttgggcgggt cgtcggtggc gcgctgggtc tcgcaggtcg gcgcgcagtt
     1441 gggtccgcag tcctcgatgc aggtgacctc ggcggtcgcg gtgcagccga tctcgcagtc
     1501 ggcgtagagc tgggcggcgc aggagacctc catggcgacc tcctcgcact tggtggtgca
     1561 ggtctcggtc ggcacgagct cgcactgcgc gtcgacggac aggtacacgc cgccacaggc
     1621 gtccaggctg gcctgcgcga cggaggggat cgagacgagc agcccgacgg ccgcgag
//
LOCUS       DASWNJ010001271         2319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_482058, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001271 DASWNJ010000000
VERSION     DASWNJ010001271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482058"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..500)
                     /locus_tag="VGB85_21340"
     CDS             complement(165..500)
                     /locus_tag="VGB85_21340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136648.1"
                     /translation="MRDHSIEASASATSCLLRLVWILVGHGALYLALGMIAANEAPLP
                     SHLDGIAAAAIAVILGARYLDIAHHGGTTVYGDPATLWHWRRHALVLLGLTAPAWLLA
                     HWLAGSPVV"
     gene            complement(659..2062)
                     /locus_tag="VGB85_21345"
     CDS             complement(659..2062)
                     /locus_tag="VGB85_21345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0136649.1"
                     /translation="MRLRTRLLLSVTAVTTAVLVASFVPLYVVIDAAETRDLDHALFR
                     HAYVAAQRLPASYVPGQPLDAGWVRVPESLEPTNRYLATYDSEGNLLAASANFADEAP
                     SLADLGVSEVSVEGTPLDVSVGQHTLRGVVLPVPGRSEVLLYAASQRSVDDDTQFLWR
                     ILLLLFVFATLATSLLARWVGERLARDVHAIAGVARAVAEGNLDARVQADARGSDETR
                     ALAADLDHMISQLSELVRSQRTFISHAAHELRSPLATLLGELQLALRRPREAGAYRGS
                     LEAMRGDVETLVHLTEDLLLLARLQAGAVPLPNPTPIESVVHDALRMARGRAEERGIE
                     VRATGLEAAGDARVPAVRGGELARLLRNLIDNAITHSAPGGVVEVAVIRGARLEVAVS
                     DSGPGVAPEDRPHIFAPFYRGAHETPEHPTGAGLGLSIARAIAQGVGGDLYLDETRTA
                     GARFVAVLPFTTSSSIA"
     gene            complement(2053..>2319)
                     /locus_tag="VGB85_21350"
     CDS             complement(2053..>2319)
                     /locus_tag="VGB85_21350"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY0136650.1"
                     /translation="HLHVATLDGKHLDLTPRELALLTYLVAHAGQVVPRTELLAKVWH
                     AGFDPGSNLVEAHVKNLRAKLGAETSTLETVRGVGYRWKVPACD"
BASE COUNT          349 a          774 c          881 g          315 t
ORIGIN      
        1 gcgccggctc gccgcgatta accgcggatt acatcttcgt aatatacgtg cgcgccgcgg
       61 tcgctgcgcc aaatcggtgg gtcgtcttca cccatacctg tacgcgcttt cgggattgcc
      121 gcgtcggata caatctgcgc agatggtcgg ctccatcgag cgcctcagac caccggcgag
      181 ccggcgagcc agtgggcgag cagccacgcc ggggccgtga ggccgagcag gaccagcgcg
      241 tgccggcgcc agtgccacag cgtcgccgga tcgccgtaca cggtcgtccc gccgtggtgc
      301 gcgatgtcga ggtaacgggc gcccaggatg acggcgatcg cggcggcggc gatgccatcg
      361 aggtgggagg gcaggggggc ctcgttcgcc gcgatcatgc cgagcgcgag gtacagcgcg
      421 ccgtggccga cgaggatcca caccagccgc aacaaacaac tcgtcgccga agcggacgct
      481 tcaatcgaat gatctcgcat gttcgccaat ttctgcgatc gccaagcgat cgcgaaatgg
      541 cggcccgacc attccagtta cctgtgacgg acgatccatc acacccgagg tgcatcatgg
      601 cagcgctacg tgaaatgtca acccgaagtg tccacattac gcgcactgat gatcatgctc
      661 atgcgatcga gctcgaggtc gtgaacggca gcaccgcgac gaagcgagcg cctgcggtgc
      721 gcgtctcgtc gaggtagagg tcaccgccga caccctgggc gatcgcgcgg gcgatcgaca
      781 ggccgaggcc ggcgccggtc gggtgctcgg gggtctcgtg cgcgccgcgg taaaacggcg
      841 caaagatgtg gggccggtcc tccggcgcga cgcccgggcc cgagtcgctg accgcgacct
      901 cgagacgggc gcctcgtatc accgcgacct cgaccacgcc gccgggcgcg ctgtgggtga
      961 tcgcgttgtc gatcaggttg cgtagcaggc gggccagctc gccgccgcgg accgccggca
     1021 cccgggcgtc gccggcggcc tcgagcccgg tcgcgcggac ctcgatgccg cgctcctcgg
     1081 cgcggccgcg ggccatgcgt agcgcgtcgt ggacgacgct ctcgatcggc gtcgggttcg
     1141 gcagcgggac ggccccggcc tgcaggcgcg cgagcagcag caggtcctcg gtcaggtgca
     1201 cgagcgtctc gacgtcgccg cgcatcgcct cgagcgagcc gcggtacgcc ccggcctcgc
     1261 gcggccggcg gagcgcgagc tggagctcgc cgagcagcgt cgccagcggc gagcgcagct
     1321 cgtgtgcggc gtgcgagatg aaggtccgct gggagcgcac gagctcgctg agctggctga
     1381 tcatgtggtc gaggtcggcg gcgagcgccc gcgtctcgtc gctgccgcgg gcgtcggcct
     1441 ggacccgcgc gtcgaggttg ccctcggcga cggcgcgggc gaccccggcg atcgcgtgga
     1501 catcccgcgc gaggcgctcg ccgacccagc gcgcgagcag cgaggtcgcg agcgtggcga
     1561 acacgaacag caacagcaag atccgccaca ggaattgcgt gtcgtcgtcg acggacctct
     1621 gcgaggcggc gtagagcagc acctcggagc gccccggcac cggcagcacg accccgcgca
     1681 gggtgtgctg accgacgctg acgtcgagcg gcgtgccctc gaccgagacc tcgctgacgc
     1741 cgaggtcggc gaggctcggc gcctcgtccg cgaagttggc ggaggcggcg agcaggttgc
     1801 cctcgctgtc gtaggtggcg agatagcggt tggtcggctc gaggctctcc ggcacgcgca
     1861 cccagccggc gtcgagcggc tggccgggga cgtagctggc gggcagccgc tgggcggcga
     1921 cgtaggcgtg gcgaaacagg gcgtggtcga ggtcgcgggt ctcggcggcg tcgatcacca
     1981 cgtagagcgg gacgaacgag gcgaccagga ccgcggtcgt cacggccgtc accgacagca
     2041 ggaggcgggt cctcagtcgc acgccgggac cttccagcga tagccgacgc cgcgcacggt
     2101 ctccagcgtg ctggtctcgg cgccgagctt ggcgcgcagg ttcttgacgt gggcctcgac
     2161 gaggttggag ccgggatcga agccggcgtg ccagaccttg gcgagcagct cggtgcgcgg
     2221 gacgacctgg ccggcgtggg cgacgaggta ggtcagcaag gcgagctcgc gcggggtgag
     2281 gtcgaggtgc ttgccgtcga gcgtggcgac gtggaggtg
//
LOCUS       DASWNJ010001272         1737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_234454, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001272 DASWNJ010000000
VERSION     DASWNJ010001272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234454"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="VGB85_21355"
     CDS             complement(<1..808)
                     /locus_tag="VGB85_21355"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0136651.1"
                     /translation="MTVVQGNTDDPGFEVDQSAAAADTQNRAGEVVDRSAHLKAAETA
                     LLRCDEVVAGYVPGVNILNGCDLYVGDGELVGIIGPNGAGKSTLLKAMFGLVTIRSGT
                     VVLRGEDITGLKAHALVRKGVGFVPQTENVFPSLTIEENLEMGSYQAPKRFAERFDFV
                     AGLFPALADRRKQRAGSLSGGERQMVAMGRALMMEPSVIFLDEPSAGLSPALQDEVFI
                     RVRQINRAGVSVVMVEQNARRCLQICDRAYVLDQGKDAYTGTGRELMKDPK"
     gene            complement(805..>1737)
                     /locus_tag="VGB85_21360"
     CDS             complement(805..>1737)
                     /locus_tag="VGB85_21360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0136652.1"
                     /translation="SSGGAMAPAAAPRRQAALAALADVAPEPGSKKPDPILVASGVTR
                     RFGGLTAVEVDHVEIQRGAITALIGPNGAGKSTFFNLLTGFDRPDDGTWSFNGQSLAG
                     VPSHKVARRGMVRTFQLTKALSRMTVMQNMLLGATGQKGEGFLSAIFPPLWRSQEQAN
                     TQRAEELLARFKLDKKRDDFAGTMSGGQRKLLEMARALMVGPEMVMLDEPMAGVNPAL
                     TQSLLGHVKGLREDGMTVLFVEHDMDMVRDISDWVIVMAQGRIIAEGPPHDIMANEAV
                     IDAYLGGHHDAPLTEAEEERVLAEAQQAHEEEVR"
BASE COUNT          235 a          596 c          633 g          273 t
ORIGIN      
        1 ccttcgggtc cttcatgagc tcacgcccgg tgcccgtgta cgcgtccttg ccctggtcga
       61 gcacataggc gcggtcgcag atctgcaggc agcggcgggc gttctgctcg accatgacca
      121 ccgagacgcc ggcgcggttg atctggcgca cgcggatgaa cacctcgtcc tgcagggcgg
      181 gggacagccc ggccgacggc tcgtcgagga agatcaccga cggctccatc atgagcgccc
      241 ggcccatggc caccatctgc cgctcaccgc cggacagcga gccggcgcgc tgcttgcgcc
      301 ggtcggccag cgcggggaac agcccggcga cgaagtcgaa gcgctcggcg aaccgcttgg
      361 gcgcctggta ggagcccatc tcgaggttct cctcgatggt gaggctcggg aagacgttct
      421 cggtctgcgg cacgaagccg acgcccttgc ggacgagggc gtgcgccttg aggccggtga
      481 tgtcctcgcc gcgcagcacg acggtgccgg agcggatcgt gaccaggccg aacatcgcct
      541 tcagcagcgt ggacttgccg gcgccgttcg ggccgatgat gccgacgagc tcgccgtcgc
      601 cgacgtagag gtcgcagccg ttgaggatgt tgacacccgg cacgtagccc gcgacgacct
      661 cgtcgcagcg cagcagcgcc gtctcggccg ccttgaggtg cgccgaccgg tcgacgacct
      721 cgccggcgcg gttctgcgtg tcggcggccg cggcgctctg gtcgacctcg aagccggggt
      781 cgtcggtgtt gccctgcacg acggtcatcg cacctcctcc tcgtgggcct gctgcgcctc
      841 ggcgagcacg cgctcctcct cggcctcggt gagcggtgcg tcgtggtggc caccgaggta
      901 ggcgtcgatg acggcctcgt tcgccatgat gtcgtgcggc ggcccttcgg cgatgatgcg
      961 gccctgggcc atcacgatga cccagtcgct gatgtcgcgg accatgtcca tgtcgtgctc
     1021 gacgaacagg acggtcatcc cgtcctcccg cagccccttg acgtggccga gcagcgattg
     1081 cgtcagggcg gggttcacgc ccgccatcgg ctcgtcgagc atgaccatct cagggccgac
     1141 catgagcgcg cgggccatct cgagcagctt gcgctgaccg ccggacatcg tgccggcgaa
     1201 gtcgtcgcgc ttcttgtcga gcttgaaccg cgcgagcagc tcctcggcgc gctgggtgtt
     1261 ggcctgctcc tgcgagcgcc acagcggggg gaagatcgcg gacaggaagc cctcgccctt
     1321 ctggccggtg gcgccgagca gcatgttctg catgaccgtc atgcgcgaca gcgccttggt
     1381 cagctggaac gtgcggacca tcccgcgccg ggcgaccttg tgggacggga cgcccgcgag
     1441 cgactgcccg ttgaacgacc aggtgccgtc gtcgggccgg tcgaagccgg tgagcaggtt
     1501 gaagaacgtg ctcttgccgg cgccgttggg gccgatgagc gcagtgatcg cgccgcgctg
     1561 gatctcgacg tggtcgacct cgacggcggt gagaccgccg aagcgccggg tcacgccgct
     1621 ggcgacgagg atcgggtcgg gcttcttgga gccgggctcg ggtgcgacgt cggcgagcgc
     1681 ggccagggcg gcctgacggc gcggggccgc agccggcgcc atggcgccgc cggagct
//
LOCUS       DASWNJ010001273         3209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_482062, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001273 DASWNJ010000000
VERSION     DASWNJ010001273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482062"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="VGB85_21365"
     CDS             complement(<1..920)
                     /locus_tag="VGB85_21365"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0136653.1"
                     /translation="MPIFRNRPSARLVLAISTIIASAVVLAYTLHWLNGPAQETTAPP
                     ARVDLPRDPIPGARPGTEDVDLAVAGITGTVRDLDGRPLAGARVCAWRLTAPWPNAER
                     ATPQCTEAAPDGFYVLPGLPPLRHAIHASAPQHRPARLDGDLSLRPGEHRGNLDFALA
                     PGGVRVAGVVQDISGGVVEGAWVSSGDGQGPGTASARSDAAGRFVLWVGAGFVHLVGQ
                     ADGYADATEYGSAPGEAYVLRLTPESVLVGEVVLADTRAPVAGARVTVIGELGEAGAV
                     GMGVTYTDAAGKFRLARLQPGVYKPRVQADG"
     gene            1078..1695
                     /locus_tag="VGB85_21370"
     CDS             1078..1695
                     /locus_tag="VGB85_21370"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY0136654.1"
                     /translation="MQRLLRVLPLLALGFIGACDEPEYEDADGNLLGEGLSDDGLIET
                     LTSELDPAAAGYCDSVTNALWNDSWETLETQVIAEINKRRLAGATCGGVAKPKVAALT
                     LNTKLRCASRKHSKDMKVNNFFSHTGSNGSTPWARIQAAGYVYTNAAENIAAGQATAA
                     AVVTGWMNSTGHCNNIMNGILKEVGVGYYPGGTYGHYWTQDFGKQ"
     gene            complement(1750..1968)
                     /locus_tag="VGB85_21375"
     CDS             complement(1750..1968)
                     /locus_tag="VGB85_21375"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEY0136655.1"
                     /translation="MHEGASLHAKCVVVDGEHTLIGSANFTDRAHTRNVEAGALTSHR
                     RFAALLIDQLHGLINAGHFLPLSSARDA"
     gene            complement(2744..2983)
                     /locus_tag="VGB85_21380"
     CDS             complement(2744..2983)
                     /locus_tag="VGB85_21380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136656.1"
                     /translation="MTDDEPGLPTSPPDSECLGPYPSLEAFVRATLAPLLPAELRWLL
                     DCLDLERVLHAMTAGGRDHLRLEGGRVYLDRLDPP"
BASE COUNT          513 a         1072 c         1176 g          448 t
ORIGIN      
        1 ccatccgcct gcacgcgcgg cttgtagacg cccggctgca gccgcgcaag gcgaaacttg
       61 cccgcggcgt ccgtgtacgt gacgcccatc ccgaccgccc ccgcctcgcc gagctcgccg
      121 atcaccgtga cccgcgcacc ggcgaccggc gcccgcgtgt cggcgaggac gacctcgccg
      181 acgagcaccg actcgggcgt caagcgcagg acgtaggcct cgcccggcgc gctgccgtac
      241 tcggtcgcgt cggcgtaacc gtcggcctga ccgacgaggt gcacgaagcc cgcgccgacc
      301 cacagcacga agcgacccgc cgcgtcgctg cgcgcgctgg cggtgccggg gccctggccg
      361 tcgccgctcg agacccacgc gccctcgacg acgccgccgc tgatgtcctg gaccaccccc
      421 gcgacgcgca cgcccccggg cgcgagcgcg aagtcgaggt taccgcggtg ctcgcctggc
      481 cgaagggaca ggtccccatc gaggcgcgcg ggtcgatgct gcggcgcgct ggcatggatc
      541 gcgtggcgca gcggcggcag gccaggcagg acatagaagc cgtcgggtgc ggcctcggtg
      601 cattgtggcg tggcgcgctc ggcgttcggc cacggcgccg tcagccgcca cgcacagacg
      661 cgtgcgcccg cgagcggccg accatcgagg tcacggaccg tgccggtgat ccctgcgacc
      721 gcgagatcga cgtcctcggt gcctggccgc gcgccgggga tggggtcgcg cgggaggtcc
      781 actctggcag gcggcgccgt cgtctcctgc gcgggcccgt tcaaccagtg gagcgtatac
      841 gcgagcacga ccgccgacgc gatgatcgtc gagatcgcga ggacgaggcg agcggacggg
      901 cggttcctga agatcggcat cgcgggtccg cccgagtatc ccatgtctcg cgcgccgaca
      961 cgcgcaaagg atctcgcgcg ccgagaatga ccgtcacgcg ggctggggtg gcccgtcccc
     1021 ggcgtgtcgc ccgggcggcc ccttgccgga attcgcggac ctctctagcg tgggagcatg
     1081 caacgactgc ttcgtgtcct cccgctgctt gccctcggat tcatcggcgc ctgtgacgag
     1141 cccgagtacg aggacgccga cgggaacctc ctcggtgagg gcctcagcga cgacgggctc
     1201 atcgagaccc tgacctccga gctcgacccg gccgcggccg gctactgtga cagcgtcacc
     1261 aacgcgctgt ggaacgacag ctgggagacg ctcgagaccc aggtgatcgc cgagatcaac
     1321 aagcgccgcc tggccggcgc gacctgtggc ggcgtcgcca agcccaaggt cgccgccctg
     1381 accctcaaca ccaagctgcg ctgcgcctcg cgcaagcact ccaaggacat gaaggtcaac
     1441 aacttcttct cccacaccgg atccaacggc tcgaccccgt gggcccgcat ccaggccgcc
     1501 ggctacgtct ataccaacgc cgccgaaaac atcgccgccg gccaggcgac cgccgccgcc
     1561 gtcgtcaccg gctggatgaa cagcaccggc cactgcaaca acatcatgaa cggcatcctg
     1621 aaggaggtcg gcgtcgggta ctacccgggt ggcacgtatg ggcattactg gacgcaggat
     1681 tttggtaagc agtgacgtgt attgaaggcg cctgagactc gttcgcgttt gggcgggccg
     1741 agatgcccgc taagcgtctc gcgcggatga cagcgggaga aagtggcccg cgttgatcag
     1801 accgtggagc tggtcgatca ggagggcagc aaagcggcgg tgggaggtca gggcgccggc
     1861 ctcgacattg cgggtgtggg cgcggtcggt gaaattggcg gagccgatca gcgtgtgctc
     1921 gccgtcgacg acgacgcatt tggcgtgcaa cgaggcgccc tcgtgcactg cgccagggag
     1981 ggcggtgcgt gggtcgtaaa acacggcggg ccggggtgca cgctgctgat agatcattcg
     2041 gccatgacgc gaaattggag gatggcgcga ggcagtcgat atagccgtcg aggcatgagt
     2101 cgacgccgcg cttggcgtcg gtcgccttgc tcaggaccca caatcgctgg gttccgtaat
     2161 atccgacgac cggcagctcg aatgtcctgc cgcgctggac atcgccggcc gcggagcggg
     2221 ccagcccgcg cagggtggtg gtcccgggtc gtcgtgcgac cgccgggctc gtcagctcgc
     2281 gggcccaggt cagcgcctcg ccaaacaggc gagcggcggc ctccacacgg actggccact
     2341 gggtctggag gtccgggacg ccgccgaggt cgtagacgat gcggcgcgcg tcgtcgtcct
     2401 cgcggaggtt acacgacggg acgtcgcgga tctcgcccac gaggcgccca gcgcgacgga
     2461 gagaccctcg aggatcccgg tcttgccagc gccgttgtcg ccgagcagga gggtccagtg
     2521 gggtggaggc tcagctcgat acgctcgaag cagcagaaat tggcgagcgt gagccggtcg
     2581 atctgcatga gccgcggggc gccgcagggt cgccgtgccg ggcaggtcgt gacaaggcgc
     2641 cggcccggcc ctgcgggggc cagatcgcgt gggctgccac tttacgtgcg gatcataggg
     2701 tggtgaggga tcggcgaggt cgtcggggcg tccgtcgcgg agctcagggc gggtcgagac
     2761 ggtcgaggta gacccggccg ccttcgaggc gcaggtggtc ccggccgccg gcggtcatgg
     2821 cgtgcaggac tcgctcgagg tccaggcagt cgagcagcca gcgcagctcg gccgggagca
     2881 gcggcgcgag cgtggcccgg acgaaggcct cgaggctggg ataggggccc aggcactcgc
     2941 tgtcgggcgg ggacgtgggg aggccgggct cgtcgtcggt catgattgtc gcggtcctga
     3001 ggggcagacg tgatcgcggg cctggtcgag aagcgcacgc cactccaggc tagactggag
     3061 tctatggaaa agcgttcatc ggcaattgag cgagcatgaa cttgggttat taacatggga
     3121 aatgcaagct gcaaagtcga ggcacgctcc gatagactcc gcccgtggca gcggtcacca
     3181 cggaggagct cgccgaggcg ctcggcatg
//
LOCUS       DASWNJ010001274         1837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_306372, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001274 DASWNJ010000000
VERSION     DASWNJ010001274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306372"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..1736
                     /locus_tag="VGB85_21385"
     CDS             213..1736
                     /locus_tag="VGB85_21385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase"
                     /protein_id="HEY0136657.1"
                     /translation="MREDRVIVIGSGLGGLAAAIRLRAMGHEVEVLEAMDQPGGRAAV
                     FKQDGFSFDAGPTVVTASYLFDELFALVGRDRRDYIEFIPVDPFYRVEFPDGGHFDYV
                     GDEDRLLAQIAAISPRDVEGYRKLAAHARQIFDVGYTQLVDKPFDRFTDMVKIVPDLV
                     RLGCWRSLYGWVSQYISDERLRQVFTFQPLLIGGNPFRSPAIYALIHWLERKWGVEFA
                     RGGTTSIVRGMVQLLAEIGVPVRCNTPVTEIEVESGKAVAVRTGAGERIACSAVVSNA
                     DPAFVYTRMVAARHRKWNRDFLVHRKKLSMGLFVGYFGARGTWPKLAHHTIILGHRYR
                     ELLEDIFERKILAEDFSLYLHAPTRSDSTLAPPGHENFYVLSPVPNTQGKVDWEAVGP
                     RYFDAILDFLDASHVPGLKANIVTRKHITPQYFETTLRSVGGAAFGPEPRLEQSAYFR
                     YHNRSEDVEGLYFVGAGTHPGAGMPGVLSSAKVLERVMQAPRVRAPVPHAVPRKAAS"
BASE COUNT          270 a          677 c          611 g          279 t
ORIGIN      
        1 caacgcgccg atgggcgagc ccgacggtgg cacccgcgtc gcccacttcg aggacgccct
       61 cgccgcggtg gtcgccggca agccggtcgc cgccgccgag accagggcgt ggggctgctc
      121 ggtcaagtac gccaagtgaa cgtgacgttt cgcagggccg cctggcgggc gaatccgccg
      181 agcttgacta tggtgcccgg gaggacagcc agatgcgcga agatcgagtg atcgtcattg
      241 gttcgggcct gggcggcctc gcagcggcga tccgcctgcg ggcgatgggc cacgaggtcg
      301 aggtgctgga ggcgatggac cagccaggcg ggcgcgcggc ggtgttcaag caggacggct
      361 tctcgttcga cgccggcccg accgtcgtca ccgcgagcta cctgttcgac gagctgttcg
      421 cgctggtcgg ccgcgaccgt cgcgactaca tcgagttcat cccggtcgat ccgttctacc
      481 gcgtcgagtt cccggacggc ggccacttcg actacgtcgg cgacgaggac cgcctgctcg
      541 cccagatcgc cgcgatcagc ccccgcgacg tcgagggcta ccgcaagctc gccgcgcacg
      601 cgcgccagat cttcgacgtc ggctacacgc agctcgtcga caagcccttc gaccgcttca
      661 ccgacatggt gaagatcgtc ccggacctgg tgcgcctcgg ctgctggcgc tcgctctacg
      721 gctgggtcag ccagtacatc agcgatgagc ggctgcgcca ggtcttcacg tttcagccgc
      781 tgctgatcgg cggcaacccg ttccgctccc cggcgatcta cgcgctgatc cactggctcg
      841 agcgcaagtg gggcgtcgag ttcgcccgcg gcggcacgac ctcgatcgtg cgcggcatgg
      901 tccagctgct cgccgagatc ggcgtgccgg tccgctgcaa cacgcccgtc accgagatcg
      961 aggtcgagag cggcaaggcg gtcgcggtgc gcaccggcgc cggcgagcgg atcgcgtgct
     1021 cggcggtcgt cagcaacgcc gatccggcct tcgtgtacac gcgcatggtc gcggcgcggc
     1081 accgcaagtg gaaccgcgac ttcctggtcc accgcaagaa gctgtcgatg ggcctgttcg
     1141 tcggctactt cggcgcgcgt ggcacctggc ccaagctcgc gcaccacacg atcatcctcg
     1201 ggcaccgcta ccgcgagctg ctcgaggaca tcttcgagcg caagatcctc gccgaggact
     1261 tctcgctgta cctgcacgcg ccgacgcgca gcgactcgac cctcgcgccg cccggccacg
     1321 agaatttcta cgtgctctcg cccgtgccga acacccaggg caaggtcgac tgggaggccg
     1381 tcggcccgcg ctacttcgac gcgatcctcg acttcctcga cgcctcgcac gtccccgggc
     1441 tcaaggccaa catcgtgacg cgcaagcaca tcacgccgca gtacttcgag acgaccctgc
     1501 gatccgtcgg cggcgcggcc ttcggaccgg agccgcggct ggaacagtcg gcctactttc
     1561 gctaccacaa ccgctccgag gacgtcgagg gcctgtactt cgtcggcgcc ggcacgcacc
     1621 cgggcgcggg catgccgggc gtgctgtcct cggccaaggt gctcgagcgc gtgatgcagg
     1681 cgccgcgcgt gcgtgcgccc gtcccccacg ccgtgccgcg caaggcggct tcctgacacc
     1741 atgacggacc tccgcgccca ggtgatcgcc gagagccgcg ccgtgctggc caggcacgcg
     1801 cgcaccttca acctcgccgg acgcttcctc ggcccca
//
LOCUS       DASWNJ010001275         1893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_515482, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001275 DASWNJ010000000
VERSION     DASWNJ010001275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515482"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VGB85_21390"
     CDS             complement(<1..189)
                     /locus_tag="VGB85_21390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136658.1"
                     /translation="MTSDERHESSLRETRGAHHTTAATSITTSRSVAQQTPPLYRQQP
                     HCAALASPTKAAYPRKRSA"
     gene            251..1246
                     /locus_tag="VGB85_21395"
     CDS             251..1246
                     /locus_tag="VGB85_21395"
                     /inference="COORDINATES: protein motif:HMM:NF039450.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADYC domain-containing protein"
                     /protein_id="HEY0136659.1"
                     /translation="MKLTKHFVTLFSSPVVISALLLATACDAEPELEAPDQPDQNGPE
                     EAPVELRPGVANGSGVYLNTSVIGADEVDLTKVLHAEVKLVSVKIKVGNKLQLLDSVW
                     AVEGELFGKIGATTYSGSAFLGAEVRVRLYTQKYYNVEVPYTIATFTPATVDEMPRYT
                     FKYWDGSAHKYLCAPDSVEAAAAIVLDSFSVNKTTGDITTRPNTIYLGCASGAVGKAP
                     TWGYTPWGLGLADFEAMVRMVRADYCGDGVSWTVPGRAVTIEDVWGINSFAAQAGATE
                     AIWGPSGALCIGQPREPSITAASVVCGGVPVPACPADVSLATYPEALAWLKSAAQ"
     gene            complement(1297..>1893)
                     /locus_tag="VGB85_21400"
     CDS             complement(1297..>1893)
                     /locus_tag="VGB85_21400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0136660.1"
                     /translation="CKTATCGDGVVQAGVEQCDDGNVVATDACTGTCATAKCGDGIIQ
                     AGVEQCDDGNAVDNDGCSNLCKVGATTCAHSACLSGAALSANSCSYTGTNNDCVAKVC
                     AIDPFCCGANGGNWDATCVAEAKDASICPTGLNPTNFSCTCAHSYCTVGGLLARTCDP
                     CVKKICELDSFCCNTSWDQACVNQTVSVCKMPAAPNCK"
BASE COUNT          276 a          590 c          710 g          317 t
ORIGIN      
        1 agcactgcgt ttgcgaggat aagctgcctt ggttggactc gccagcgcag cgcaatgcgg
       61 ctgttgcctg tacagcggcg gcgtttgctg cgcgacggat ctcgaggtgg tgatcgacgt
      121 cgcggcggtc gtgtggtgag caccgcgtgt ctcgcgtagc gacgattcat ggcgctcgtc
      181 gctcgtcatc gcgctcgagt ggttctatct cgggcgtgta caacaccgcc tgcgagcgga
      241 ggagacagcc atgaagctca cgaaacattt tgtcactctt ttctcctctc cagtggtgat
      301 cagcgcgtta ctgctggcga cggcctgcga cgccgagccc gagctcgagg cgcccgacca
      361 gcccgaccag aatgggccgg aggaggcccc tgtcgagctg cgccccggcg tggccaacgg
      421 tagcggcgtg tacctcaaca ccagcgtgat cggtgcggac gaggtcgacc tgaccaaggt
      481 gctgcatgcc gaggtgaagc tggtcagcgt caagatcaag gtcggcaaca agctgcagct
      541 gctcgactcg gtgtgggcgg tcgagggcga gctgttcggc aagatcggcg cgacgaccta
      601 cagcggctcg gccttcctcg gcgccgaggt gcgggtccgg ctgtacacgc agaagtacta
      661 caacgtcgag gtgccgtaca cgatcgccac cttcacgccg gcgaccgtcg acgagatgcc
      721 gcgttacacg ttcaagtact gggacggctc ggcgcacaag tacctgtgcg cgccggactc
      781 ggtcgaggcg gcggcggcga tcgtgctcga ctcgtttagc gtcaacaaga cgaccggcga
      841 catcacgacc cgccccaaca cgatctacct cggctgtgcg tcgggcgcgg tcggcaaggc
      901 gccgacctgg ggctatacgc cgtgggggct cgggctcgcg gacttcgagg cgatggtccg
      961 gatggtgcgc gccgactact gcggcgacgg ggtgtcgtgg acggtccccg ggcgcgcggt
     1021 gacgatcgag gacgtgtggg ggatcaacag cttcgcggcg caggcgggcg cgaccgaggc
     1081 gatctggggg ccgagcgggg cgctgtgcat cggtcaaccg cgcgagccga gcatcaccgc
     1141 cgcgagcgtc gtgtgcgggg gcgtgccggt gccggcgtgc ccggcggacg tcagcctggc
     1201 gacgtacccc gaggcgctcg cctggctcaa gtcggcggcg cagtgagagc gcgcgcgccc
     1261 gttgcgacgc cggtcgtcgc aacgggcggg gcggcgtcac ttgcagttcg gggccgcggg
     1321 catcttgcac acggagaccg tctggttgac gcaggcctgg tcccagctgg tgttgcagca
     1381 gaacgagtcg agctcgcaga tcttcttgac gcaagggtcg caggtacgag ccagcaggcc
     1441 gccgaccgtg cagtagctgt gcgcgcaggt gcagctgaag ttggtcgggt tgaggccggt
     1501 cgggcagatg ctggcgtcct tggcctcggc gacgcaggtg gcgtcccagt tgccgccgtt
     1561 ggcgccgcag cagaacgggt cgatcgcgca gaccttggcg acgcagtcgt tgttggtgcc
     1621 ggtgtagctg cagctgttgg cgctcagggc ggcgccggag aggcaggccg agtgcgcgca
     1681 ggtggtcgcg ccgaccttgc acaggttgct gcagccgtcg ttgtcgaccg cgttgccgtc
     1741 gtcgcactgc tcgacgccgg cctggatgat gccgtcgccg cacttggcgg tcgcgcaggt
     1801 gccggtgcag gcgtcggtgg cgacgacgtt gccgtcgtcg cactgctcga cgccggcctg
     1861 gacgacgccg tcgccgcagg tggcggtctt gca
//
LOCUS       DASWNJ010001276         2342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_619998, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001276 DASWNJ010000000
VERSION     DASWNJ010001276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619998"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2281)
                     /locus_tag="VGB85_21405"
     CDS             complement(<1..2281)
                     /locus_tag="VGB85_21405"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY0136661.1"
                     /translation="MLELFTCRNCGTAHARAYTNNVDAPDFLWGEPGGAFRTLAGFVD
                     ELSPLDLLLEKPVFHNLAEPAEYDLITGRLNPQKLGARNRQVYIRAGRSQPVTDDEPP
                     DAKPGELRPCAVCGGSASFGRTSVQDHQTKGDQPFQALIAKQIQVQPPSPVLQTRLAP
                     LRGRKVLIFSDSRQTAARLAPNIQTYSTQDAIRPLIVAGYERLLGTAAIAPFLSLDDL
                     YFGVLIAAKELGVRLRPELKAGESFQDELDVDVAIKGGALTNPAALMLLWGKLRASRP
                     PESLLRAVVNSLGDRYLGLEALAIASLRERGDHTTWIETLPDIPSFATRAEQKLATAR
                     SWMRCWGRAGLSMSQMPPAWRNSGRQFQARSGKFDEMERLIADKLARSFFNKEWLPKL
                     LQRFGEQVAPNKFYLRGSELSLQVGGSWAYCQSCKTSQRPFPGHTICVNCGQPTATPI
                     DPNTDKVFVARKGYYRASTIDALKIPPVPPMALIAAEHTAQLNTAQAVEVFSKAEEHE
                     LLFQDVDLGVDGTGHDRPAIDVLSCTTTMEVGIDIGSLSGVSLRNMPPARANYQQRAG
                     RAGRRGNAVATVTAFGSADSHDEHYFSNPDEMIRGAVDDPLLTLDNSEIIRRHVTAYL
                     LQRYHQDRLPTINPEEQPQLFAVLGTVADFKKSAKVLNRWDLKKWLDANETTLRAEVD
                     AWLPQQFSAAERKRLLDELVQKTMEPIDAAIELEPPAGAPASPPAPTAASETPTTATV
                     DTALEVPDEEGEEKPRRDPA"
BASE COUNT          376 a          749 c          791 g          426 t
ORIGIN      
        1 aggcggggtc gcgcctcggc ttctcctcgc cttcctcgtc agggacctcg agcgcggtgt
       61 ctacggtggc cgtcgtgggc gtctcggatg cggcggtcgg cgcaggtggg gatgcgggtg
      121 cccctgcggg aggctcgagc tcgatcgccg cgtcgatcgg ctccatcgtc ttctgcacga
      181 gttcatcgag gaggcgcttt cgctccgcag cggagaactg ctgcgggagc cacgcgtcga
      241 cctcagcgcg aagcgtcgtc tcgtttgcgt cgagccactt cttgaggtcc catcgattga
      301 gcaccttcgc cgacttcttg aagtcggcca cggtgccaag caccgcgaag agttgcggtt
      361 gctcctccgg atttatcgtg gggagcctgt cctggtgata acgctggagg aggtaagcag
      421 tgacgtgtcg cctgatgatc tcgctgttgt cgagagtgag caacgggtcg tcgaccgcgc
      481 cgcggatcat ctcgtcgggg ttcgagaagt agtgctcgtc gtgactgtcc gcgctgccga
      541 acgccgtcac cgtcgcgacc gcgttgccgc ggcgacccgc gcgaccagca cgctgctggt
      601 agttcgcgcg tgcaggcggc atgttccgca gcgacacacc ggagagcgac ccgatgtcga
      661 tacccacctc catcgtcgtc gtacaggaca gcacatcgat cgcgggccga tcgtgcccgg
      721 tcccatcgac gccgaggtcc acgtcctgga acaggagttc gtgctcctcg gccttcgaga
      781 agacctcgac ggcctgcgcc gtgttgagtt gcgcggtgtg ctctgccgcg atgagcgcca
      841 tcggcggcac cggcggtatc ttcagcgcgt ctatggtgct cgcgcggtag tagcccttgc
      901 gcgcgacgaa gaccttgtcg gtgttgggat cgatgggcgt cgcggtgggc tggccgcagt
      961 tcacacagat cgtgtgacca gggaacgggc gctgcgaggt cttgcaggac tggcagtagg
     1021 cccacgagcc gccgacctga agcgagagct cactgcctct caggtagaac ttgttcggcg
     1081 cgacctgctc accgaagcgc tggagcagct tgggcagcca ctctttgttg aagaaggaac
     1141 gcgcgagctt gtccgcgatg agccgctcca tctcgtcgaa cttgcccgaa cgcgcctgga
     1201 actggcggcc tgagttgcgc catgcgggtg gcatttggct catcgataag ccggcacgcc
     1261 cccaacaccg catccatgag cgcgcggtcg ctagcttctg ctccgcccga gtagcgaagc
     1321 ttgggatgtc tgggagggtc tcgatccacg tagtgtggtc cccgcgctcc cggagcgacg
     1381 cgatagcgag tgcctcgagg ccgaggtagc ggtcgcccag cgagttcacg accgcgcgca
     1441 acagcgactc cggtggccgt gacgcgcgta gcttgcccca gaggagcatt aatgccgcag
     1501 gattagtgag ggcaccgccc ttgatagcga cgtccacgtc gagttcatcc tggaagctct
     1561 ctcccgcctt gagctccggc cgaagcctga cacctaactc cttcgcggcg atgagcacgc
     1621 cgaagtacag gtcgtcgaga gacagaaacg gggctatcgc ggctgtcccc aacagccgtt
     1681 cgtagcccgc gacgatgagc ggacggatcg cgtcctgagt cgagtaggtc tggatgttcg
     1741 gtgcgagacg cgccgcagtc tggcgagagt ccgaaaagat gaggaccttg cgcccgcgca
     1801 gcggcgcgag ccgcgtctga agcacagggc tcggcggctg gacttggatc tgcttagcga
     1861 tgagcgcctg gaacggctgg tcaccctttg tctgatgatc ctggacagac gttcgcccga
     1921 acgacgcgct ccctccgcat acagcgcagg ggcggagctc gcccggcttc gcgtccggcg
     1981 gctcatcgtc cgtcacgggc tgggaccgcc ccgcccggat gtagacctgc ctgtttcgcg
     2041 cgccgagctt ctgcgggttc agcctcccgg tgatcaggtc gtactcggcg ggctcggcca
     2101 ggttgtggaa gacgggtttc tcaaggagaa ggtcgagcgg ggagagctcg tcgacgaacc
     2161 cggccagcgt ccgaaatgcg ccgcccggct ctccccagag gaagtccgga gcgtcgacat
     2221 tattagtgta cgcgcgcgca tgggccgtac cgcagttcct gcacgtgaac agctccagca
     2281 cgcgcgcacc acactcacac gagtcccgcg gctggctgta gagcttgccc gcaggaccgc
     2341 cg
//
LOCUS       DASWNJ010001277         1564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_127865, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001277 DASWNJ010000000
VERSION     DASWNJ010001277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127865"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..526)
                     /locus_tag="VGB85_21410"
     CDS             complement(170..526)
                     /locus_tag="VGB85_21410"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY0136662.1"
                     /translation="MYEISSHGRIRSLRYVSDGQPLVMSLVLTKGYLQTRVTMDDGRK
                     KTIFAHRAVCEIWHGPCPSPAHVVAHRRDVRTLNTPDELHWATRSENARAAIENGAHI
                     TRRGYVRGHQGDLTVF"
     gene            complement(852..1442)
                     /locus_tag="VGB85_21415"
     CDS             complement(852..1442)
                     /locus_tag="VGB85_21415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136663.1"
                     /translation="MKTETKTTIPRKYLLENNIPHSLLPGNEASIGCDYLRMVAAVLT
                     YAAKPVSLAPEFNKRNMNKVAQLCCEKVQHTPRPYPKLDQQRDRIVCAFLFVVAFPRL
                     GGGDGAAGRGLLNGPLPKGVGLALWRAHENLREVVAARLPMLAEAIRAFGPTMSAPVN
                     LDAKTLDMFLQIGQGIDALVDAWEGQESLAAHRRAG"
BASE COUNT          257 a          481 c          508 g          318 t
ORIGIN      
        1 gcagctcggc gccctcgcat acctccgaca ccgggacctt ctcgaggtgg tggcggcgca
       61 ggaggttaat tttctggtct ggggtgtggt gacggcgtgt cttcttgctc atgggctctc
      121 cttgcccggc tgcttttacc aggccggggc gtgtcccatt ccagctgagc taaaacaccg
      181 tcaggtcgcc ctggtgaccg cgcacgtaac cgcgacgggt gatgtgcgcg ccgttctcga
      241 tcgcggctcg ggcgttctcg ctgcgcgtcg cccagtggag ttcatcgggg gtgttcagcg
      301 tgcgcacgtc acgacggtga gccacaacgt gggccggact cgggcagggg ccgtgccaga
      361 tctcgcagac ggcacgatga gcgaagatcg tcttcttgcg gccgtcgtcc atcgtgacac
      421 gggtctgcaa gtaacccttc gtcagtacga gggacatcac cagcggctgg ccgtcgctca
      481 cgtagcgcag cgaccgaatg cggccgtggc tgctgatctc gtacatggat gcgccgatgg
      541 gcgcgaggga acgccactct tcgggggcgt cggaggttcg gttgttactg tcagttgtca
      601 tctgccatcc actcacgccg gctcaatgcc tggcgttcaa tccggccctc accattgcac
      661 ggggttacgg gccaccctac ggtcgccgtt gtgaggcggc tcagctcggg gtttgcgatc
      721 ggggcggctc gatgagcaca aacgacgaaa cccgccggaa gcgggtctcg cacgatgcct
      781 tgtgatgtgt tgtcgggtta cgtgccgtcg cgtgtagtga tgtcgcgtgc cgtggagttg
      841 ccgtggtgtg gtcatcccgc acggcggtga gccgcgaggg attcttggcc ctcccaagca
      901 tcgaccaagg catcgatgcc ctgaccgatc tgtagaaaca tgtccagcgt cttggcgtcg
      961 agattgacgg gagcggacat cgtaggcccg aatgccctga tcgcttcggc aagcatcggc
     1021 aggcgagcag cgacgacttc ccggaggttc tcgtgcgctc gccacaaggc cagcccgacg
     1081 cccttgggca aaggcccgtt gagaagacct cgaccggcag cgccgtcacc tccacccaac
     1141 ctcggaaagg ccaccacgaa caggaacgca caaacaatcc ggtcgcgctg ctgatcgagc
     1201 ttggggtacg gccgcggggt gtgctgcact ttctcacagc agagctgagc gaccttgttc
     1261 atgttccgtt tgttgaactc cggtgccagg ctcaccggtt tcgcagcgta ggtcaggacc
     1321 gctgcgacca tccgaagata atcacagccg atggatgcct cgttgccggg gaggaggctg
     1381 tgagggatat tgttttccag caggtacttt cgcgggatgg tcgtcttcgt ctcggttttc
     1441 atcgtctact ccactcggcg gcgctcaagg tgctccgctc cctccccttg tactgatctg
     1501 cggccctgcc tgacaaggaa aaagagcagg cttcggcagc tgcaatcctc acagggcgcg
     1561 ggcc
//
LOCUS       DASWNJ010001278         3633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_515524, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001278 DASWNJ010000000
VERSION     DASWNJ010001278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515524"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VGB85_21420"
     CDS             complement(<1..838)
                     /locus_tag="VGB85_21420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136664.1"
                     /translation="MLRLHRSLIVLAASFASACPAGGDESTTGSSSTSTSTSPTTGTS
                     DAPTTSGTTSTDTPTTSTTDTPATTTTTDPGTTTDLATSTGSETGDPVVSDCETPPCF
                     NVINKCAFPLWIHAANNDQVVLQPDNVELAPGGVQQYPVPAEWPAGRVNAFYAEPNAN
                     PEAHDKVEMTVTGGIMNYNITYVDYVALPSEMQAVGPECQATPEFDPKVGCYVPRDQL
                     LDGCPDDLLTGERCLSASLYCADPAHQALPYCHALDGEIAKCAAQHPETCGVAAQLGN
                     TTR"
     gene            1079..2527
                     /locus_tag="VGB85_21425"
     CDS             1079..2527
                     /locus_tag="VGB85_21425"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEY0136665.1"
                     /translation="MQPNKQLNRLFAALGREGLEVSREDRVYRVRRKEEGGPPAEVLL
                     PDDFPVEARALRQLAGLMAVRHPAGGSVERVCASPDFHPGDAGVAIGSIAVTEGMVIP
                     AAVGSDINCGMRLHVVDLAPDALLARRDELVELLKGDFFFGTRDLPMAAASMRALFHE
                     GLLGWIDSVSRRPLGVLAGSDFAQISAELERVHLGGALDGHARWAPEDLAPAHGQVRD
                     GGMATIGGGNHFVEIQVVESVVHGGLAHAWGVKAGQLAFMIHSGSRGVGKHVGAAWRD
                     KARAAWPKGQRHPDEALFALSLADRPELVASYLEAEATAANYGFVNRLLLAELLRLRL
                     RQLFGAGVSAPLVYDLPHNITLPAQGPARRWITRKGACPADAGQPVIIPGSMGAASYL
                     AVGRGSSKLLSSASHGAGRARSRFMMSNHSDQDERALGLTGVDCITLREERRVEEAPA
                     AYKPIEPVIACQVEAGTIDVVARLRPLMTFKA"
     gene            2625..>3633
                     /locus_tag="VGB85_21430"
     CDS             2625..>3633
                     /locus_tag="VGB85_21430"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEY0136666.1"
                     /translation="MIATTLAACEGLGAAQPADPVDPMFMRDEPALPSAPARRIPEAA
                     PVVRSPLARRLEAFAAEVAARRAERVAPKPGGQPQDLTIAAGPPAVFDVAGLDAAIRK
                     IVAPLERSTDISVHIRELASEDVLFDYRGDAPLNPASNHKLLTAAAALDLLGADFRFE
                     TRVVLHDSALHVIGEGDPTLDDLALRELAAEVAGAVDPGSLRKIVVDDLVFSPRTLAP
                     GFSDEGVGEAYQAPSGALSLDFNTVEVTAVAPRVKTKPGELVPLQVTLRPQSPHVVVD
                     NRAVVGRGAVTVRSYAGMELAGQARTVVELTGKLRAGRTLVERRRVVDPGLYTGGALA
                     VM"
BASE COUNT          459 a         1217 c         1384 g          573 t
ORIGIN      
        1 cccgggtcgt gttgccgagc tgggccgcga cgccgcaggt ctccgggtgc tgcgcggcgc
       61 acttggcgat ctcgccgtcg agggcgtggc agtagggcag ggcctggtga gcaggatctg
      121 cgcagtagag gctggcggac aggcagcgct cgccggtcag caggtcgtcc gggcagccgt
      181 cgagcagctg gtctctcggc acataacagc cgaccttcgg gtcgaactcg ggggtggcct
      241 ggcactcggg accgacggcc tgcatctccg acggcaaggc gacataatcg acgtaggtga
      301 tattataatt catgatcccg ccggtcaccg tcatctccac cttgtcgtgg gcctccgggt
      361 tggcgttcgg ctcggcgtag aaggcattga cccgacccgc gggccactcg gcaggcacgg
      421 gatactgctg cacgcccccg ggggcgagct cgacgttgtc cggctggagc accacctgat
      481 cgttgttggc cgcgtggatc cacagcggga acgcgcactt gttgatcacg ttgaagcacg
      541 gcggcgtctc gcagtcgctg acgaccgggt cacccgtctc gctgccggtc gaggtagcga
      601 ggtcggtggt ggtgccgggg tccgtggtcg tcgtggtcgc gggcgtgtcg gtcgtgctgg
      661 tcgtgggcgt gtccgtgctc gtggtcccgg aggtggtcgg cgcgtcgctc gtgcccgtgg
      721 tcggcgaggt ggatgtcgag gtgctcgagc tgccggtcgt gctctcgtcc ccgccggcgg
      781 ggcaggcgga ggcgaacgag gccgcgagga cgatcaggga gcggtggagc cggagcaccg
      841 cgcgatctta catcgatcaa aaattcctcg gatcggtcaa aattgttttg aagctttgga
      901 gtcggcctgc gtataaggcc ccaacgcttc cgcggctgct aactgtcgga gtgtccatgt
      961 ttgccggtcg tgcctcgttg gggcggccgt gtcgggtcca gggcgcatgt gcgccggggt
     1021 ccagggatcg tgcctttgcg tgccttgtgg gttcgcgggg gcgcgggagt gacgtgtcat
     1081 gcagccgaac aaacaattga accgactgtt cgcggcgctc ggtcgtgaag ggttggaggt
     1141 cagccgtgag gaccgggtct accgggtgcg gcgcaaggag gagggcggtc cgccggcgga
     1201 ggtgctgttg ccggacgatt tcccggtcga ggccagggcg ctgcggcagc tcgcgggcct
     1261 gatggccgtg cgtcaccctg cggggggctc ggtggagcgc gtgtgcgcgt cgccggactt
     1321 tcatcccggc gacgcggggg tggcgatcgg atcgatcgcc gtcaccgagg ggatggtgat
     1381 ccccgccgcg gtcgggtccg acatcaactg cggcatgcgt ctgcacgtcg tcgacctcgc
     1441 gccggacgcc ttgctggcgc ggcgcgacga gctggtcgag ctgctcaagg gggacttctt
     1501 cttcggtacg cgcgacctgc cgatggccgc ggcgtcgatg cgcgcgctgt tccacgaggg
     1561 cctgctcggg tggatcgaca gcgtgtcgcg gcggccgctc ggcgtgctcg cgggctcgga
     1621 cttcgcccag atctctgcgg agctggagcg cgtgcacctc ggcggcgcgc tcgacgggca
     1681 cgcgcgctgg gccccggagg acctggcgcc cgcgcatggg caggtccgcg acggcggcat
     1741 ggcgacgatc ggtggcggga accacttcgt cgagatccag gtcgtcgagt cggtcgtgca
     1801 cggcgggctc gcgcacgcgt ggggcgtcaa ggcggggcag ctcgcgttca tgatccactc
     1861 gggctcgcgc ggggtcggca agcacgtcgg cgccgcgtgg cgcgacaagg cgcgcgccgc
     1921 ctggcccaag ggacagcgtc atccggacga ggcgttgttc gcactgtcgc tcgccgaccg
     1981 gcccgagctg gtcgccagct acctcgaggc cgaggcgacc gcggccaact acggcttcgt
     2041 caaccggctg ttgctcgccg agctgctgcg gctgcggctg cgccagctgt tcggggcggg
     2101 cgtctcggcg ccgctcgtct acgacctgcc gcacaacatc accttgcccg cgcagggtcc
     2161 ggcgcgccgc tggatcaccc gcaagggggc ctgtccggcc gacgccgggc agccggtgat
     2221 catcccgggc tcgatgggcg cggcgtccta cctcgcggtc gggcgcggca gctcgaagct
     2281 gctgtcgtcc gcctcgcacg gggcgggtcg ggcccgctcg cgcttcatga tgtcgaacca
     2341 cagcgaccag gacgagcgcg cgctcgggct gaccggggtg gactgcatca ccctgcgcga
     2401 ggagcgtcgc gtcgaggagg cgccagcggc gtacaagccg atcgagccgg tgatcgcctg
     2461 tcaggtcgag gccgggacga tcgacgtggt cgctcgcctg cgcccgctca tgaccttcaa
     2521 ggcgtgagcc gacgcgcgca cggttgtcga cccgccgccg ggctgcgatg ctcggcggcg
     2581 ggagcgtgcg aatacacgga caggccagcc ggcgtttgct cgtcgtgatc gcgacgaccc
     2641 tcgctgcatg tgaggggctt ggcgcggcgc agcccgccga cccggtcgat ccgatgttca
     2701 tgcgcgacga gccggcgctc ccgagcgcgc cagcgcggcg gatccccgag gccgcgccgg
     2761 tcgtgcgctc gccgctggct cggcgcctcg aggccttcgc ggccgaggtg gccgcgcgcc
     2821 gggccgagcg cgtcgccccg aagccgggcg ggcagccgca ggacctgacg atcgccgcgg
     2881 ggcctccggc tgtgttcgac gtcgccggcc tcgacgcggc gatccgcaaa atcgtcgcgc
     2941 cgctcgagcg cagcacggac atttcggtgc acatccgcga gctcgcctcg gaggacgtgc
     3001 tgttcgacta tcgcggcgac gcgccgctca acccggccag caatcacaag ctgttgaccg
     3061 ccgcggccgc gctcgatctg ctgggcgccg actttcgctt cgagacgcgc gtggtcctgc
     3121 acgacagcgc gctgcacgtc atcggcgagg gcgacccgac cctcgacgac ctggcgctgc
     3181 gcgagctggc agcggaggtc gcaggcgcgg tcgacccggg cagcctgcga aagatcgtcg
     3241 tcgacgacct cgtgttctcg ccgcgcacgc tcgcgccggg cttctccgac gagggcgtcg
     3301 gcgaggccta tcaggcgccc agcggcgcgc tcagcctcga cttcaatacg gtcgaggtga
     3361 cggccgtcgc tccccgcgtc aagacgaagc ccggcgagct cgtgcccctg caggtgacgc
     3421 tgcgcccgca gagcccgcac gtcgtcgtcg acaaccgggc cgtcgtcggc cgcggcgccg
     3481 tcacggtgcg cagctacgcg ggcatggagc tggctgggca ggcccgcacg gtcgtcgagc
     3541 tgacgggcaa gctgcgcgcc ggtcggacgc tggtcgagcg ccgccgcgtc gtcgaccccg
     3601 ggctgtacac cggcggggcg ctggcggtga tgc
//
LOCUS       DASWNJ010001279         2578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_234559, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001279 DASWNJ010000000
VERSION     DASWNJ010001279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234559"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..610)
                     /locus_tag="VGB85_21435"
     CDS             complement(128..610)
                     /locus_tag="VGB85_21435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136667.1"
                     /translation="MRGLSAAAGLGLAIGLVAACYNDDFLLGALCGRDTDCGEDQCCA
                     GRRCRPQPDDCYLLEADNDQPFVAAYMACDDDAACLEHGMPRCVLWQGAARGFCADLC
                     AGAPSLYCNRHVFLAPVDTLPRTCVDVDGQSLCALDCSQNNLCPDEMQCHAGVCVPTP
                     "
     gene            720..1949
                     /gene="murG"
                     /locus_tag="VGB85_21440"
     CDS             720..1949
                     /gene="murG"
                     /locus_tag="VGB85_21440"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEY0136668.1"
                     /translation="MPALMSSAPLVLPRTLLHAAVADPPETTHLEVPASDETRVRGTR
                     PLRVLIAGGGTGGHLFPGVALAEEVRARGGEVLFVGTQRGIEARVLPEQGWSLQTIDA
                     TGIKGRGLRGLFTGLLRLPRAWWQSVKIVRGFRPDVVVGVGGYASGPVVATAAMLLRP
                     TAILEQNSIPGITNRILSRLVRRVFCTFPDRGGFFPRRKVVLSGNPIRRPILAALQAA
                     RGEASAPVSRAPRLFVFGGSQGARAINDMMIAALPGLLARVPGLEIWHQTGRGDQERV
                     AAAYRGLGLESPRVRVADFLTDMATPYAWSDLVLCRAGATSLSELAAAGRPAVLVPFP
                     QATDNHQEHNAHALVEANAAVLLREREWSAATLADTLAGLLADPARLAAMREAMLAAA
                     RPAAAGSIYAELVALCS"
     gene            complement(1982..2527)
                     /locus_tag="VGB85_21445"
     CDS             complement(1982..2527)
                     /locus_tag="VGB85_21445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136669.1"
                     /translation="MSMFHVRTFAVFTLLLSSCDTDVAPLPVSTPDPAAAMSLRAELP
                     AIELAGGELVALDLTDEQQASLECAGDPRISQLWIGPLQLGERELAAPVLEVACDGEH
                     AQPQLSLRETGAPSVRDELPLRAPEPMPVNTCDWAGTTVCVPCGGIGMRTRTTVVTNY
                     NPYTNHCTYGYTYGGCSVNAC"
BASE COUNT          339 a          887 c         1003 g          349 t
ORIGIN      
        1 gcctcggcct ggatcccgag ctcgtaggcg aggtcgcgct cggacggggc ggcctgcgcg
       61 gaacctgtcc cgagggacag gctgagcgcg agcgcgaggc tcgtcaggcg agtgagggcc
      121 gggcgcgtca cggtgtcggc acgcacacgc ccgcgtggca ctgcatctcg tcggggcaga
      181 ggttgttctg cgagcagtcg agcgcgcaca gcgactggcc gtcgacgtcg acgcaggtgc
      241 gcgggagggt gtcgacaggg gcgaggaaca cgtgccggtt gcagtacagg ctcggcgcgc
      301 cggcgcagag gtccgcgcaa aagccccgcg cggcgccctg ccagagcacg cagcgcggca
      361 tgccgtgctc gaggcaggcg gcgtcgtcgt cgcaggccat gtaggcggcg acgaagggct
      421 ggtcgttgtc ggcctcgagc aggtagcagt cgtcgggctg gggtcgacag cgccggccgg
      481 cgcagcactg gtcctcgccg cagtcggtgt cgcgcccgca cagggctccg agcaggaagt
      541 cgtcgttgta gcaggcggcg accagcccga tcgccaggcc gagccccgcc gccgctgaca
      601 ggccacgcat cacccggcag ggtagcgcgc ggcggcgcgg cggtacaggc cgcgcggcgc
      661 ccctggcggg ttttgccata aacctgcgag cccgggcgta atgcgctacc aagcccacca
      721 tgcccgcgct catgtcttct gcccccctgg tgctgccgag gaccctcctg cacgccgccg
      781 tcgccgatcc gccggagacc acccacctgg aggtcccggc gagcgacgag acgcgggtcc
      841 gcggcacgag gccgctgcgc gtgctgatcg cgggcggtgg gacgggcggg cacctgttcc
      901 ccggcgtcgc gctcgccgag gaggtgcggg cgcgcggcgg cgaggtgctg ttcgtcggca
      961 cgcagcgcgg catcgaggcc cgcgtattgc ccgaacaggg ctggagcttg cagacgatcg
     1021 acgcgacggg gatcaagggc cgcggcctgc gcgggctgtt caccggcctg ttgcggctgc
     1081 cgcgggcgtg gtggcagagc gtcaaaattg tccgcggctt tcggcccgat gtcgtcgtcg
     1141 gggtcggcgg ttatgcgagc ggtccggtgg tcgcgaccgc ggcgatgctg ctgcgaccga
     1201 cggcgatcct cgagcagaac agcatccccg ggatcaccaa ccggatcctt tcgcggctgg
     1261 tgcggcgggt gttctgcacg ttccccgatc gcggagggtt cttcccgcgg cgcaaggtgg
     1321 tgctcagcgg caacccgatc cgccggccga tcctcgcggc gctgcaggcc gcgcgcgggg
     1381 aggcgagcgc gccggtctcg cgagcgccgc ggctgttcgt gttcggcggc tcgcaggggg
     1441 cgcgggcgat caacgacatg atgatcgccg cgctgccggg gctgctcgcg cgcgtgcccg
     1501 ggctcgagat ctggcaccag accggacgcg gcgatcagga gcgggtcgcg gcggcctacc
     1561 gcgggctcgg gctcgagtca ccccgcgtgc gcgtcgccga ctttttgacc gacatggcga
     1621 ccccctacgc gtggtcggac ctggtgctgt gccgcgccgg cgcgacctcg ctgtccgagc
     1681 tggcggccgc cggacgaccc gcggtgctgg tgccgttccc gcaggcgacc gataatcacc
     1741 aggagcacaa cgcccacgcg ctcgtcgagg cgaacgcggc ggtgctgctg cgcgagcgcg
     1801 agtggtcggc ggcgacgctc gccgatacgc tcgccgggct gctcgcggac ccggcccgac
     1861 tcgccgcgat gcgcgaggcg atgctcgcgg cggccaggcc cgccgcggcg gggtcgatct
     1921 acgccgagct cgtcgcgctg tgctcgtgag ggccggcgct cgacatggat ggtgacgccg
     1981 cctagcaggc gttgacgctg cacccgccgt aggtgtagcc gtaagtgcaa tgattggtgt
     2041 aggggttgta gttggtcacg acggtggtgc gcgtccgcat cccgatgccg ccgcacggca
     2101 cgcagacggt ggtgcccgcc cagtcgcacg tgttcacggg catcggctcg ggcgcccgca
     2161 gcggcagctc gtcgcggacc gacggcgcac cggtctcgcg caggctcagc tgcggctgcg
     2221 cgtgctcgcc gtcgcaggcg acctcgagca ccggcgccgc gagctcgcgc tcgccgagct
     2281 gcagcggccc gatccacagc tgcgagatgc gcgggtcgcc ggcgcactcg aggctcgcct
     2341 gctgctcgtc ggtgaggtcg agcgcgacga gctcaccgcc cgcgagctcg atcgcgggca
     2401 gctcggcgcg cagcgacatc gcggcggcgg ggtccggcgt cgacacgggg agcggcgcca
     2461 cgtcggtgtc acagctggac aggagcaggg tgaagacggc gaatgtacgg acatgaaaca
     2521 tggacatggg acctcggcga acggtaacag aggcggcccg cgcctcgacg cgggtcga
//
LOCUS       DASWNJ010001280         3366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_659366, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001280 DASWNJ010000000
VERSION     DASWNJ010001280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659366"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..547)
                     /locus_tag="VGB85_21450"
     CDS             complement(233..547)
                     /locus_tag="VGB85_21450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEY0136670.1"
                     /translation="MKVEAAITEKLREAFAPAELRVENQSDQHSGPRGRETHFKVVVV
                     SERFDKQSLVQRHRMVNATLAEELRGGVHALSIEALTPAQWEARGGLVAASPPCLGGS
                     KS"
     gene            complement(544..2685)
                     /locus_tag="VGB85_21455"
     CDS             complement(544..2685)
                     /locus_tag="VGB85_21455"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY0136671.1"
                     /translation="MYKQIAFAVVVAFSLGLFTVTMTHMVRIIKLGRPSELKETWAER
                     IASLMAFFFGQGKVVEERRSWHHLPIYWGFLILTIATVDLGLGGLLGSWFNLGVLLGD
                     TAYGWLKAVIDWANLMVFAALMYAFVRRFRRPAFVPLSLDAMLILGAITMLVVSHFGM
                     HAFEIAATAAYQPGSPVSGRIGDWLGLYDFNGSSVSVHVDQNVAHYSVEVFWWVHMGI
                     VLAFLNYLPFSKHIHVLGSGPNILLRKQGQRGAMPKAKLLPDDPEGDPLFENWGVGKV
                     EDFTWKSLLDNYACTECARCTAYCPAFATEKPLSPMHMIHDLKDEMRDRGGLLVKLKK
                     AGGTLNDEVEPGPGADKDKIAKIKAELQGLPPLVGGRVKDETLWACTTCGACQEVCPV
                     FIDHPLKILQMRQHIVLNDESGRTPGEVTKVLGNISSAGNPWRFNQDDRMNWAEGLDV
                     KTVEDQPDAEYLLFIGCAGAFDDNAKKTSRALVKVLNAANVKFAVLGKREKCSGDPVR
                     RLGAEMDYQTMAQENVDMFNEAKVTKVIASCPHCFHTIKNEYPQFGGNFEVIHHSQLI
                     SHLLTTGKLKVETPSDKQFTYHDSCYIGRWNGVYDAPRDILDHVTKGKGSVRELDRNK
                     EHGFCCGAGGGRMWMEEEPSKRVNINRATEVAASGANAVAVACPFCNTMLSDGLKHLG
                     RDEDIAVVDLAVAIAASLPDAPRVTSAEAQA"
     gene            complement(2821..>3366)
                     /locus_tag="VGB85_21460"
     CDS             complement(2821..>3366)
                     /locus_tag="VGB85_21460"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEY0136672.1"
                     /translation="GLHSNGYSLARKALLDPAHGGLTLHDPLPGDPAGRSVGESLLIP
                     TRIYEYAMGPLRRLADAPLHGAAHITGGGLLENPQRALADSLALTLDLTEVPCPPVLA
                     AIAAQGVPPRELLRTFNGGIGMILYVDPEQEALVQSTLRRVGEEAIPLGRVVARGSAD
                     EPQVRIRGGAWLGGADFTSEG"
BASE COUNT          492 a         1146 c         1175 g          553 t
ORIGIN      
        1 ctcgccaccg gcggcaccgc gggcgctgcc tctcgtctga tcgagaagct cggcggcacc
       61 gtcgcggcct gcctgttcct gatcgagctc gcgggcctcg agggccgcgc caagctcggc
      121 gagcgacgcg tcgagtcgat catcgtgtac tgaactggca tgtgcgggcc tggcgttcgg
      181 ccggaatttg cccaacgccg agcaggtgcc ccggagcccg ggccgcgcgc gatcagctct
      241 tcgacccgcc gaggcaaggc ggcgaagcag cgacgagccc gccgcgagct tcccactgcg
      301 cgggcgtcag cgcctcgatc gacagcgcat gcacgcctcc gcgcagctcc tccgcgagcg
      361 tcgcgttgac catgcgatgc cgctgcacga gcgactgctt atcaaagcgc tcgctgacga
      421 cgacaacttt aaaatgtgtc tcacgcccgc gcggccccga gtgctggtcg ctttgattct
      481 cgacgcgcag ctccgcgggc gcgaacgcct cgcggagctt ctcggtgatc gcagcctcga
      541 ccttcacgcc tgcgcctccg cggacgtgac ccgcggagcg tcgggcaggc tcgccgcgat
      601 ggcgaccgcg aggtcgacga ccgcgatgtc ctcgtcgcgg ccgaggtgct tgaggccgtc
      661 gctgagcatc gtgttgcaga acgggcaggc gaccgcgacc gcgttggcgc ccgaggccgc
      721 gacctcggtg gcgcggttga tgttgacgcg cttgctcggc tcctcttcca tccacatgcg
      781 gccgccgccg gcgccgcagc agaagccgtg ctccttgttg cggtcgagct cgcgcacgga
      841 ccccttgccc ttcgtgacgt ggtcgaggat gtcgcgcggc gcgtcgtata cgccgttcca
      901 gcggccgatg tagcagctgt cgtggtacgt gaactgcttg tcgctcgggg tctcgacctt
      961 gagcttgccg gtcgtcagca ggtggctgat cagctggctg tggtggatga cctcgaagtt
     1021 gccgccgaac tgcgggtatt cgttcttgat cgtgtggaag cagtgcgggc acgaggcgat
     1081 caccttggtg accttggcct cgttgaacat gtcgacgttc tcctgcgcca tcgtctggta
     1141 gtccatctcg gcgccgaggc ggcggaccgg gtcgccgctg cacttctcgc gcttaccgag
     1201 caccgcgaac ttcacgttgg cggcattgag caccttgacg agcgcgcgcg acgtcttctt
     1261 ggcgttgtcg tcgaacgcgc cggcgcagcc gatgaacagc aggtactcgg cgtcgggctg
     1321 gtcctcgacc gtcttgacgt cgaggccctc ggcccagttc atgcggtcgt cttgattgaa
     1381 acgccacggg ttgccggcgc tgctgatgtt gccgagcacc ttggtcacct cgccgggcgt
     1441 gcggccggac tcgtcgttga ggacgatgtg ctggcgcatc tgcaggatct tgagcgggtg
     1501 gtcgatgaac accgggcaca cctcctggca cgcgccgcag gtggtgcagg cccacagggt
     1561 ctcgtccttg acgcgaccgc cgacgagcgg cggcaggccc tgcagctccg ccttgatctt
     1621 ggcgatcttg tccttgtcgg cgccggggcc gggctcgacc tcgtcgttga gggtcccgcc
     1681 ggccttcttg agcttgacga gcagaccgcc gcggtcgcgc atctcgtcct tgaggtcgtg
     1741 gatcatgtgc atcggcgaca gcggcttctc ggtcgcgaac gcagggcagt aggccgtgca
     1801 gcgggcgcac tcggtgcacg cgtaattgtc gagcaggctc ttccaggtga agtcctcgac
     1861 cttgccgacg ccccagttct cgaacagcgg gtcgccctcg gggtcgtccg gcagcagctt
     1921 ggccttgggc atcgcgccgc gctggccctg cttgcgcagc aggatgttgg ggcccgagcc
     1981 gagcacgtgg atgtgcttgg agaacgggag gtagttgagg aacgcgagca cgatccccat
     2041 gtggacccac cagaacacct cgacgctgta atgcgcgacg ttctggtcga cgtgcaccga
     2101 cacggagctg ccgttgaagt cgtagagccc gagccagtcg ccgatccggc ccgacaccgg
     2161 gctaccgggc tggtaagccg cggtagcagc gatctcgaag gcgtgcatgc cgaagtggct
     2221 gacgacgagc atcgtgatcg cgccgaggat cagcatcgcg tcgaggctca gcggcacgaa
     2281 cgcggggcgc cggaagcggc gcacgaacgc gtacatcagc gcggcgaaga ccatcaggtt
     2341 ggcccagtcg atcaccgcct tgagccagcc gtacgcggta tcgccgagca gcacgccgag
     2401 gttgaaccag ctgccgagca ggccgccgag cccgaggtcg acggtggcga tcgtcaggat
     2461 caaaaagccc cagtagatcg gcaggtggtg ccagctgcgg cgctcctcga cgaccttgcc
     2521 ctggccgaag aagaacgcca tcaagctggc gatccgctcg gcccaggtct ccttgagctc
     2581 cgaggggcgg ccgagcttga tgatccgcac catgtgcgtc atcgtgacgg tgaagagccc
     2641 cagcgagaac gcgacgacga cggcgaaggc gatctgtttg tacatgacgc tgccgcctgc
     2701 atagcacagg agcgccagtc cgcgctcgcg gcccgaaccc gccccaggcc cgccagagcg
     2761 ctccaaaacc gtcggggccc gggaaaggcc tgtgcgcttg ctccgcgtcc gcgcccgtcc
     2821 tcagccctcg ctggtgaagt cggcgccgcc cagccaggcg ccgccgcgaa tgcgaacctg
     2881 cggctcgtcg gcgctgccgc gggcgaccac gcggcccagc gggatcgcct cctcgccgac
     2941 gcgccgcagc gtcgactgca cgagcgcctc ctgctcgggg tcgacgtaca ggatcatgcc
     3001 gatgccgccg ttgaaggtcc gcagcagctc gcgcggtggt acgccctggg cggcgatcgc
     3061 cgcgagcacg ggcgggcagg gcacctccgt cagatcgagg gtcagcgcca gcgagtccgc
     3121 gagcgcccgc tgcgggttct cgagcaagcc gccgccggtg atgtgcgcgg cgccgtgcag
     3181 cggcgcgtcg gcgaggcggc gcagcgggcc catcgcgtac tcgtagatcc gcgtcgggat
     3241 cagcagcgac tcgccgacgc tgcgcccggc cgggtcgccg ggcagtgggt cgtgcagcgt
     3301 cagcccgccg tgcgccgggt cgagcagggc cttgcgcgcc aggctgtagc cgttgctgtg
     3361 caggcc
//
LOCUS       DASWNJ010001281         2100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_554399, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001281 DASWNJ010000000
VERSION     DASWNJ010001281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554399"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VGB85_21465"
     CDS             <1..194
                     /locus_tag="VGB85_21465"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HEY0136673.1"
                     /translation="SRLIRVAACDSPAFAHYKPGPAGGHVPWSLIVLELAGDGIAAMT
                     YFLDTQALFPRFGLPLALP"
     gene            279..707
                     /locus_tag="VGB85_21470"
     CDS             279..707
                     /locus_tag="VGB85_21470"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0136674.1"
                     /translation="MSTPTVTSRKLFVNLPVQDLQRSIAFFTRLGFRFNPHFTDATAT
                     CMIVSEEAYVMLLVRDRFKDFTNKQICDTAAATEGLFALSATSRAEVDELVHRAIDAG
                     GSHAMPPQDHGFMYGWSFYDPDGHHWEVFYMDPAAIPPNP"
     gene            866..1471
                     /locus_tag="VGB85_21475"
     CDS             866..1471
                     /locus_tag="VGB85_21475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136675.1"
                     /translation="MNATTATPSLARRSLRAPLLWMAVLAAPVAVAAAVSTSATSAFA
                     AEAEPAEESAKISTRVTSKGVVIAEMRVHAKAAAVRELLAGAERAHGLASTTVSVKAS
                     PDGKCEKVKLQTRGLITPFVLETRRCPTATGWRETLVTSDNFVEYWNEWTVKDLGDGA
                     LVTFKTRTLPNVAVPESLILSQTRKVLGKLMKSVLVALGET"
     gene            complement(1495..>2100)
                     /locus_tag="VGB85_21480"
     CDS             complement(1495..>2100)
                     /locus_tag="VGB85_21480"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEY0136676.1"
                     /translation="GLELWPRDPGGEQCITLEQRILDSKQATNPACEVGEILVPPALG
                     SGAAITSAIDPLTTHICSTTPTASGLITASDWLIDHVVPGRKQYIVLVTDGADWSFSC
                     PDPSPLLTTQQIAAAGIQTYVVGFFGQEAQAGALAFLNDMACAGQTAKDFANNCVQMG
                     AGYVAKDPMFPTPLYLQAGDNELPATLEGVAAEILQFCVPG"
BASE COUNT          305 a          782 c          683 g          330 t
ORIGIN      
        1 gttcgcgact gatccgcgtc gccgcctgcg actcaccggc cttcgcccac tacaagcccg
       61 ggcccgccgg cggccacgtc ccctggtccc tgatcgtgct cgagctggcc ggcgacggca
      121 tcgccgcgat gacctacttc ctcgacacgc aggccctgtt cccccgcttc ggcctgcccc
      181 tcgcgctgcc ctgagcggcc cgaagagatt ttttgccggc gaccgatgag tccgcgcggg
      241 cgcggccgtc tatcgcacag aacccggaac ggagccccat gagcaccccc accgtcacgt
      301 cccgcaagct cttcgtcaac ctgcccgtgc aggacctcca gcggtcgatc gcgtttttta
      361 cccgcctcgg ctttcgcttc aacccgcact tcaccgacgc caccgcgacc tgcatgatcg
      421 tcagcgagga ggcctatgtc atgctgctgg tccgcgaccg cttcaaggac ttcaccaaca
      481 agcagatctg cgacaccgcg gccgccaccg agggcctgtt cgcgctcagc gccacgagcc
      541 gggccgaggt cgacgagctg gtgcaccggg cgatcgacgc cggtggctcg cacgccatgc
      601 cgccgcagga ccacggcttc atgtacggct ggagcttcta cgaccccgac ggccaccact
      661 gggaggtctt ttacatggac cccgcggcca tcccgccgaa cccgtgatcg ccggaggcag
      721 cacgcgaggg cgaggacgcg ccccgcgttg cgccgccctc gcacgcacaa gtccgatcgc
      781 ggcccggatc ctcgccatgg atccgtgcgc gggcgcctgc acggccaccg gcacacccgt
      841 gtctttgggt ctatagtccc cgatgatgaa cgcgacgacc gccacgccct ccctcgcacg
      901 tcgctctctg cgtgccccgt tgttgtggat ggcggtgctc gcggcgccgg tcgctgttgc
      961 ggcagcggtc agcacctctg ccacctcggc attcgccgcc gaggccgagc ccgctgagga
     1021 gtcggcgaag atctcgacgc gggtcacctc caagggcgtc gtcatcgccg agatgcgtgt
     1081 acacgccaag gccgccgccg tgcgcgagct gctggccggc gccgagcggg cccacggcct
     1141 cgcctcgacc accgtgtcgg tcaaggccag cccggacggc aagtgcgaga aggtcaagct
     1201 gcagacccgc ggcctgatca cgcccttcgt gctcgagacg cgccgctgcc cgaccgcgac
     1261 cggctggcgc gagacgctcg tcacctccga caacttcgtc gagtactgga acgagtggac
     1321 cgtcaaggac ctcggcgacg gcgccctcgt caccttcaag acccgcaccc tgcccaacgt
     1381 cgcggtgccc gagtcgctga tcctctcgca gacccgcaag gtgctcggca agctgatgaa
     1441 gagcgtgctc gtcgcgctcg gcgagacctg agccttcgct tcctacctcc ggttttagcc
     1501 cggcacgcag aactggagga tctcggcggc gacgccctcg agcgtcgccg gcagctcgtt
     1561 gtcgccggcc tgcaggtaca gcggcgtcgg gaacatcggg tccttggcga cgtagccggc
     1621 gcccatctgc acgcagttgt tcgcgaagtc cttggcggtc tggccggcgc aggccatgtc
     1681 gttgaggaac gcgagcgcgc cggcctgggc ctcctggccg aagaagccga ccacgtaggt
     1741 ctggatgccc gccgcggcga tctgctgggt cgtcagcagc ggcgaggggt ccgggcagct
     1801 aaagctccag tcggcgccgt cggtgacgag cacgatgtac tgcttgcggc ccggcacgac
     1861 gtggtcgatc agccagtccg acgccgtgat cagcccggag gcggtcggtg tcgtcgagca
     1921 gatgtgggtc gtcagcgggt cgatcgccga cgtgatcgcc gcgccgctgc ccagcgccgg
     1981 cggcaccagg atctcaccga cctcgcaggc ggggttggtc gcctgcttgc tgtcgaggat
     2041 gcgctgctcg agcgtgatgc actgctcgcc gcccgggtcg cgcggccaca gctcgaggcc
//
LOCUS       DASWNJ010001282         3403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_620170, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001282 DASWNJ010000000
VERSION     DASWNJ010001282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620170"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VGB85_21485"
     CDS             <1..618
                     /locus_tag="VGB85_21485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136677.1"
                     /translation="PYVKSANVDEQAIIDAVVGMPMVGDEYGLIPAFSVYLANMPADF
                     YNQICIGFVEAMRALGRDKAAMRLLVSDAEHCGMNTFRGIMHSPEWDGLIAPMVQGRA
                     DALHAIIAISNALGWGDWHVTAHTPLTLALESRYGYEAVGYREHRGEAHNPQCAMLTG
                     VAAGVMELVYGEGTVEERFGTFASREERCISCGDASCNFNVERVE"
     gene            624..893
                     /locus_tag="VGB85_21490"
     CDS             624..893
                     /locus_tag="VGB85_21490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136678.1"
                     /translation="MRREKVPACVHPETYKYYLEREDVLETWRRLAERYGQPRRHAGF
                     RTMEIGAPRFILRSTLLGNPITVLRRRDCTPSDILELHALLGFLG"
     gene            890..2746
                     /locus_tag="VGB85_21495"
     CDS             890..2746
                     /locus_tag="VGB85_21495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0136679.1"
                     /translation="MSTVGGAAPWAFALELDDQLRILRVDPALWERLGCTANDVAGQS
                     LDELFSLRDRKGQRLFYEGMSRADEAGLDLLITLEIGDREVLTRLQMAPREVGWVAQI
                     EPLTADGNLVYQLFSAQERWAHIVKRSTEGVAVLDAEGRIVDSNASFFELMQFRSPHG
                     VILSEEALRGRSLRPLLDSHGSGLAPLAQHLGGPGVARERFAAALAWGDLWLDVTATP
                     LQLPVRGYVGVCVTIRDVTERRQTESLLRQKEAAEAANLAKTRFLANMSHELRTPLNA
                     IIGYSDMLLEEAEDDGMDAARVTDLRRIGTAGVHLLELINNILDLSKIEAGRMELWPE
                     TFSARALVQGVIAALEPLAHRRGNRMELRVPREIGFMDTDMLKLRQVLFNLLGNAIKF
                     TEDGTIRVHVQRMTIEERDWIEIAVVDSGIGIDAEALPRLFQDFTQADGSTTRRYGGA
                     GLGLSIAREFCTLMGGKITAESTPGEGSTFTVLVPAELGPAKVTRAVAPVQRPILVID
                     DDLAVLDQVQRALQREGLFVVSCSSPAQGASVARHLRPQAIVLGVGLGDRTAWTTLAQ
                     LRGEVGLRGVPVLVASLLDEQARAIGLGASAYLSKPIDRAHLLSALAQLRGP"
     gene            complement(2756..3265)
                     /locus_tag="VGB85_21500"
     CDS             complement(2756..3265)
                     /locus_tag="VGB85_21500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136680.1"
                     /translation="MLRAHVVRGAALCVAVTVVPPPAAASPWSPRLVQAPATPAAGAE
                     PAAPAAGVEPAAPAPPDPADIPRPVRRRGGDDDEEPRDPPPGDGTGMLVSGIFMLGLG
                     VVLLGFGVARGDERDDYGGTLMLIGGVPLTIAGTSLTIAGGVRRHRYNQTAEQARLQL
                     GPGGLRLEF"
BASE COUNT          499 a         1180 c         1248 g          476 t
ORIGIN      
        1 ccctacgtga agtcggccaa cgtcgacgag caggcgatca tcgacgcggt cgtcggcatg
       61 ccgatggtcg gcgacgagta cggcctgatc ccggcgttta gcgtgtacct ggcgaacatg
      121 cccgcagatt tctacaacca gatctgcatc ggcttcgtcg aggcgatgcg ggcgctcggg
      181 cgcgacaagg cggcgatgcg gctgctggtc tcggacgccg agcactgcgg catgaacacc
      241 tttcgcggga tcatgcactc gccggagtgg gacggcctga tcgcgccgat ggtccagggc
      301 cgggccgacg ccctgcacgc gatcatcgcg atcagcaacg cgctcggctg gggcgactgg
      361 cacgtcaccg cccacacgcc gctgaccctc gcgctcgagt cgcgctacgg ctacgaggcg
      421 gtcggctacc gcgagcaccg cggggaggcc cacaacccgc agtgcgccat gctgaccggc
      481 gtcgccgcgg gcgtgatgga gctggtctac ggcgagggca ccgtcgagga gcgctttggc
      541 acctttgcct cccgcgagga gcgctgcatc agctgcggcg acgcctcgtg caacttcaac
      601 gtcgagcggg tggagtgaag gccatgcgac gcgagaaggt ccccgcctgc gtacacccgg
      661 agacctacaa gtattatctc gagcgcgagg acgtgctgga gacgtggcgg cgcctcgcgg
      721 agcgctacgg ccagccgcgc cggcacgccg gctttcgcac gatggagatc ggcgcgccgc
      781 ggttcatcct gcgttcgacg ctgctgggca acccgatcac cgtgctgcgc cgccgggact
      841 gcacgccctc ggacatcctc gagctccacg cgctgctggg attccttgga tgagcaccgt
      901 cggtggcgcg gcgccgtggg cgttcgccct cgagctcgat gatcaactgc ggatcttgcg
      961 ggtggatccg gcgctgtggg agcgcctggg gtgcacggcg aacgacgtcg cggggcagag
     1021 cctcgacgag ctgttctcgc tgcgtgaccg caagggccag cgcctgttct acgaggggat
     1081 gagccgcgcc gacgaggccg gcctcgacct gctgatcacc ctcgagatcg gcgaccgcga
     1141 ggtgctcacg cggctgcaga tggccccacg cgaggtaggc tgggtcgcgc agatcgagcc
     1201 gctgacggcc gacggcaacc tcgtatatca gctgttctcg gcgcaggagc ggtgggctca
     1261 catcgtcaag cggtcgaccg agggcgtcgc cgtgctcgac gccgagggcc ggatcgtcga
     1321 ctcgaacgcg agcttcttcg agctgatgca gtttcgttcg ccgcacggcg tgatcctcag
     1381 cgaggaggcg ctgcgcgggc ggtcgctgcg gccgttgctc gacagccacg gctcgggcct
     1441 cgcgccgctc gcccagcacc tcggcggccc cggcgtcgcg cgcgagcgct tcgcggcggc
     1501 gctggcgtgg ggcgacctgt ggctcgacgt caccgcgacg ccgctgcagc tgccggtgcg
     1561 cggctacgtc ggcgtgtgcg tgacgatccg cgacgtcacc gagcggcgcc agaccgagag
     1621 cctgctgcgc cagaaggagg ccgcggaggc ggccaacctc gccaagacgc gcttcctcgc
     1681 caacatgagc cacgagctgc gcacgccgct caacgcgatc atcggctaca gcgacatgtt
     1741 gctcgaggag gccgaggacg acggcatgga cgccgcgcgc gtcaccgacc tgcgccggat
     1801 cggcaccgcc ggcgtgcacc tgctcgagct gatcaacaac atcctcgacc tgagcaagat
     1861 cgaggcgggg cgcatggagc tgtggcccga gacgtttagc gcccgcgcgc tggtgcaggg
     1921 cgtgatcgcg gcgctcgagc cgctggcgca tcgccgcggc aaccgcatgg agctccgcgt
     1981 cccgcgcgag atcggcttca tggacaccga catgttgaag ctgcgccagg tgctgttcaa
     2041 cctgctcggc aacgcgatca agttcaccga ggacggcacg atccgcgtgc acgtgcaacg
     2101 catgacgatc gaggagcgcg actggatcga gatcgcggtc gtcgacagcg ggatcgggat
     2161 cgacgcggag gcgctgccgc ggctgttcca ggacttcacg caggccgacg gctcgacgac
     2221 ccgccgctac ggcggcgccg gcctcggcct gtcgatcgcg cgcgagttct gcacgctgat
     2281 gggcggcaag atcaccgccg agagcacgcc gggcgagggc tcgaccttca ccgtcctcgt
     2341 gcccgccgag ctcgggcctg cgaaggtcac ccgcgcggtc gccccggtgc agcggccgat
     2401 cctcgtgatc gacgacgacc tggcggtcct cgaccaggtc cagcgcgccc tgcaacgcga
     2461 gggcctgttc gtcgtcagct gctcgagccc ggcgcagggc gccagcgtgg cccgccacct
     2521 gcggccgcag gcgatcgtgc tcggcgtcgg cctcggtgat cgcaccgcct ggacgacgct
     2581 ggcccagctg cgcggcgagg tcggcctgcg cggcgtgccg gtgctcgtcg cctcgttgct
     2641 cgacgagcag gcgcgcgcga tcgggctcgg tgcgtccgcg tatctgagca agccgatcga
     2701 ccgggcgcac ctgctcagcg cgctcgccca gctgcgtggg ccgtgagccc ggcggtcaga
     2761 actcgagtcg gaggccgccg ggaccgagct ggagccgggc ttgctcggcg gtctgattat
     2821 agcggtggcg tcggacccca ccggcgatcg tcaggctggt gccggcgatc gtcagcggga
     2881 ccccgccgat cagcatgagg gtgccgccgt agtcgtcgcg ttcgtctccc cgcgcgacgc
     2941 cgaagccgag cagcaccacg ccgagcccga gcatgaagat gccactgacg agcattccgg
     3001 tgccgtcgcc cggcggcggg tcgcgcggct cctcgtcgtc gtcgcctcca cgtcgtcgta
     3061 cggggcgcgg gatatcggcg gggtcggggg gcgcaggtgc ggcgggctcg accccggcag
     3121 cgggcgcggc gggctcggct cccgcagcgg gcgtggccgg ggcctgcacc agccgcggcg
     3181 accacggcga ggccgcggcc ggaggtggaa cgacggtgac ggcgacgcac agcgcggcgc
     3241 cgcggacgac gtgcgcgcgg agcatgccgg tacgataccc tcgccgtatg cgaggccgca
     3301 gtgaccgcct ccacagccgc ggggggagag gaggccacac gaacgaccgg agcagccgcg
     3361 cggggcactt gtgatacctt tgcgcgcgaa agcgagaatg aac
//
LOCUS       DASWNJ010001283         1554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_554448, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001283 DASWNJ010000000
VERSION     DASWNJ010001283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554448"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VGB85_21505"
     CDS             complement(<1..180)
                     /locus_tag="VGB85_21505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136681.1"
                     /translation="MTTRPQEARAAREGSAAKDPLLGATFAGRYTIRRLLARGGVGLV
                     YLAQQRDPDRDVVVKV"
     gene            complement(458..529)
                     /locus_tag="VGB85_21510"
     tRNA            complement(458..529)
                     /locus_tag="VGB85_21510"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(495..497),aa:Cys,seq:gca)
     gene            complement(647..1408)
                     /locus_tag="VGB85_21515"
     CDS             complement(647..1408)
                     /locus_tag="VGB85_21515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136682.1"
                     /translation="MSNTENSALIALSELKNLEANRVAEIESAKKAKEEAERRAREEA
                     ERIAKAEADRLAKLAAEQEARDREERLRLQEADLRARAEQEARLKEEQMRLDAQVKLA
                     EKKAKPVWLYAVLGLLVVGLAGGGYWAYDNAQRVAEETRIAKAAQEKRDAEQKAAMES
                     LVAELKGLKDEQDKLQKQKQELDDKLNNVTDAAEKSRLLAEKAALDAQLADNASKQTR
                     KRGQSSKPAAGGDDKPAKSKGNVSVTKTDDPLDGL"
BASE COUNT          198 a          498 c          569 g          289 t
ORIGIN      
        1 caccttgacg acgacgtcgc ggtccgggtc gcgctgctgg gcgaggtaca ccaggccgac
       61 gccgccgcgc gcgagcaggc gacggatcgt gtagcgtccg gcgaaggtcg cccccagcag
      121 cgggtctttc gcggcggagc cctcgcgggc tgcgcgtgct tcctgtggcc tggtcgtcat
      181 cggcatacgt gcgaggaccc gaacgatcca cgcagcaaac gaatggtaac gccggccgtg
      241 acgcctgtct cgctcgccgt ggcgcgcaga tcacagccgc ctggcgcaac acgggcgcgg
      301 tgactgcgtg tgaacaacgt tcacgagcac ggcacgcggc gacgccgggc gagagcacgc
      361 gacgcatgcg atgcgtcgtg tcgcggtgcg ccagaaacga cgaagccccc gcgggcgaac
      421 cggcgggggc tttgtggatc ctcgaggatc gattgagagg cctgggccgg aattgaaccg
      481 gcgtatagag cttttgcaga gctctgcctt accacttggc taccaggccg gtagcgggga
      541 gcctgtctaa caaagatcag agcgccgggc aaggggctgt ttcctcgccg gcgctcccgt
      601 ttatggtgcc agctcgcgac ggacgccgcg aggaccggag atcggatcag aggccgtcga
      661 gcgggtcgtc ggtcttggtg accgagacgt tgcccttgga cttcgcgggc ttgtcgtcgc
      721 cgccggccgc gggcttgctg gactggccgc gcttgcgggt ctgcttgctg gcgttgtcgg
      781 cgagctgggc gtccagggcg gccttctcgg cgagcaggcg ggacttctcg gccgcgtcgg
      841 tgacgttgtt gagcttgtcg tcgagctcct gcttctgctt ctggagcttg tcctgctcgt
      901 ccttgaggcc cttgagctcg gcgaccagcg actccatcgc ggccttttgc tcggcgtccc
      961 gcttctcctg ggcggccttg gcgatgcggg tctcctcggc gacgcgctgg gcgttgtcgt
     1021 aggcccagta gccgccgccg gcgaggccga cgacgagcag gccgagcacg gcgtacagcc
     1081 acacgggctt ggccttcttc tcggcgagct tgacctgggc gtcgaggcgc atctgctcct
     1141 ccttgaggcg ggcctcctgc tccgcacggg cgcgcaggtc ggcctcttgc aggcgcaggc
     1201 gctcctcgcg gtcacgggcc tcttgctccg cggcgagctt ggcgaggcgg tcggcctcgg
     1261 ccttggcgat gcgctcggcc tcctcgcggg cgcggcgctc ggcctcctcc ttggccttct
     1321 tcgcggactc gatctcggcc acgcggttgg cttcgaggtt cttgagctcg ctgagtgcga
     1381 tcagggctga gttctcggtg ttggacatgg cgactaactc ctggacgact ggaaggcggc
     1441 ggcttccggg ggcggtcaac gcatcacggc ccgttacggc ttgagggctt gtgggcttgc
     1501 gggcttgcgt tgaaaattaa tgcggggatc gaggcgagtg cggcccgaag acag
//
LOCUS       DASWNJ010001284         2167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_200299, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001284 DASWNJ010000000
VERSION     DASWNJ010001284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200299"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VGB85_21520"
     CDS             <1..739
                     /locus_tag="VGB85_21520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136683.1"
                     /translation="QRPDSPLALIDFGGVRMALAPDGGSTMIGTFGYMAPEQLHGEAT
                     AATDIYALAATVAALAAGIPADRLPRRGLTIDLSQVLPDGPLRAVLTQMLAPEPAQRL
                     GSVAAVRQALAHAHLSPPPRPAGHVPPDRSHATGGTGRQDLAAAPEHALTPHRMPRVL
                     AFVIWFWTLMAAGMFWMIEMVALPLFFTLVGQSRRYRKPNRRKRLADRQQRATLAVRG
                     VRRSIEAMSERFDPSRPALPPGDDERR"
     gene            809..1474
                     /locus_tag="VGB85_21525"
     CDS             809..1474
                     /locus_tag="VGB85_21525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136684.1"
                     /translation="MRASPVLTLALLAALPACEAVDGFFTEVPRTYSSAGNVQRIFAD
                     DFSRAELGPEWRTTGPGARIVDGALEVEGLRNHPVWLARPLPDALRIEFDATALADEG
                     DIKVELAGDGESFARAASYVASGYVMIFGGWNNSTNALVRRDEHGGDRRTANRPKVEP
                     GRRYHIVLTRDDGQIRWELDGEELLTYDDPDPLVGPGQQHFAFGGWEARVRFDNLVIS
                     SLD"
     gene            1497..>2167
                     /locus_tag="VGB85_21530"
     CDS             1497..>2167
                     /locus_tag="VGB85_21530"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0136685.1"
                     /translation="MRLLALSDLHVGYGVNREALAGLAACPDDWLILGGDLGETGEHL
                     RFVFDTLVPRFRQLVWVPGNHELWTSREHGQLLGGLAKYEHLVALCRSYGVLTPEDPY
                     PVWTGEGGPHLLVPMFLLYDYSMRPDDIPQAQARQWAAEAGIVAADEFRIEAAPHANV
                     PDWCAARLETTQARLAHAPSLPKILINHWPLREDLIHLPRIPRYTPWCGTRATHDWHR
                     RYDAAV"
BASE COUNT          286 a          846 c          744 g          291 t
ORIGIN      
        1 gcagcggccc gactcgccgc tggcgctgat cgacttcggc ggcgtgcgca tggccctcgc
       61 gcccgacggc ggctcgacga tgatcggcac cttcggctac atggcgcccg agcagctgca
      121 cggcgaggcg accgcggcga ccgacatcta cgcgctcgcg gcgaccgtcg cggcgctcgc
      181 ggccggcatc ccggccgacc gcctgccgcg ccgcggcctg acgatcgacc tctcgcaggt
      241 gctgcccgac ggcccgctgc gcgccgtgct gacccagatg ctcgcgccgg agccggccca
      301 gcgcctcggc agcgtcgccg cggtgcgcca ggcgctcgcg cacgcccacc tgagcccgcc
      361 gccacgcccc gcgggacatg tccctccgga caggtcccac gcgacaggcg gcaccggtcg
      421 ccaggacctc gccgccgccc cggagcacgc gctgacgccc caccgcatgc cgcgcgtgct
      481 cgccttcgtg atctggttct ggaccctgat ggccgccggc atgttctgga tgatcgagat
      541 ggtcgcgctg ccgctgttct tcacgctcgt cggacagagc cgccgctacc gcaagcccaa
      601 ccgccgcaag cgcctcgccg accggcagca gcgggcgacg ctcgcggtcc gcggggtgcg
      661 gcgcagcatc gaggcgatgt ccgagcgctt cgacccgagc cgccctgccc tgccgcccgg
      721 cgacgacgag cggcgctgat gtgatccggt gcttgcggac gccgcgacgg ttgcatttcg
      781 cggcgcccgg gccataccct gcgacaccgt gcgcgcgtcg cctgtcctca ccctcgccct
      841 gctggccgcg ctgcccgcct gcgaggccgt ggacggcttc ttcaccgagg tcccgcgcac
      901 ctacagcagc gccggcaacg tgcagcggat cttcgccgac gacttctcgc gtgccgagct
      961 cgggcccgag tggcgcacca ccgggccggg cgcgcgcatc gtcgacggcg cgctcgaggt
     1021 cgagggcctg cgcaaccacc ccgtgtggct ggcccggccg ctgcccgatg cgttgcggat
     1081 cgagttcgac gcgaccgcgc tcgccgacga gggggacatc aaggtcgagc tcgccggtga
     1141 cggcgagtcg ttcgcgcgcg cggccagcta cgtggcgagc ggctacgtga tgatcttcgg
     1201 cggctggaac aacagcacca acgcgctggt gcgccgcgac gagcacggcg gcgaccggcg
     1261 caccgccaac cgcccgaagg tcgagcccgg gcgccgctac cacatcgtgt tgacgcgcga
     1321 cgacggccag atccgctggg agctcgacgg cgaggagctg ctcacctacg acgaccccga
     1381 cccgctggtc ggcccgggcc agcagcactt cgcgttcggc ggctgggagg cgcgcgtgcg
     1441 cttcgacaac ctcgtgatct ccagcctcga ctgacgattc accaagccac cgacccatgc
     1501 gcctgctcgc cctcagcgac ctccacgtcg gatacggcgt caaccgcgag gcgctcgcgg
     1561 ggctggccgc gtgccccgac gactggttga tcctcggcgg tgatctcggc gagaccggcg
     1621 agcacctgcg cttcgtgttc gacaccctcg tgccgcggtt tcgccagctc gtgtgggtgc
     1681 ccggcaacca tgagctgtgg acctcgcgcg agcacggcca gctgctcggc gggctcgcca
     1741 agtacgagca cctggtcgcg ctgtgccggt cgtacggcgt gctgacgccc gaggacccgt
     1801 accccgtgtg gaccggcgag ggcggcccgc atctgctcgt cccgatgttc ctgctgtacg
     1861 actacagcat gcgcccggac gacatcccgc aggcgcaggc gcgtcagtgg gccgccgagg
     1921 ccggcatcgt cgcggccgac gagtttcgca tcgaggccgc cccgcacgcg aatgtcccgg
     1981 actggtgcgc ggcgcggctg gagacgacgc aggcccgcct cgcgcacgcg ccgagcctgc
     2041 cgaagatcct gatcaaccac tggcccctgc gcgaggacct gatccacctg ccccggatcc
     2101 cgcgttacac cccctggtgc ggcacgcggg cgacccacga ctggcaccgc cgatatgacg
     2161 cggcggt
//
LOCUS       DASWNJ010001285         4552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_515636, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001285 DASWNJ010000000
VERSION     DASWNJ010001285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515636"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..3541
                     /locus_tag="VGB85_21535"
     CDS             92..3541
                     /locus_tag="VGB85_21535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HEY0136686.1"
                     /translation="MPSPAYAFSTRIHIMIANKVRESLVDSGNGKIALRLGEHSVTLT
                     AADIEALSDHPLSFRAGAVGPDNMVFPGMTDPSHALGQRPFEQCELLYQAAELPEERA
                     YALGCFLHGSTDAVAHHYVNYMTGETFTLTPITSNRESSFDNVVRHILAESQIQAAAI
                     AQDPGAFTDSRLLHSIPIGFVLRAYLDQDSPLWQMMAAHPKAEYDKVLAEDPEASLPT
                     IVAKMEVGPADHLVLSPVYLASVDDLIVKKKIELMDRIATMQDWNSPEGSQLKVTAGD
                     DGTIGTKDDETDCSASCPELFATYFTFVGLLAPRYNAQNQELPSAFEKVSEQLRKELF
                     LFHEAYLEVVAGLSAKLNAAPNEMSGEFGVNKEELKVLFQPLNDWADKLSTIDYDTLV
                     YATVPEWFIELDTFMQAFGVDFDIAALIKAVFDPIIQPIKDAIKQAFIEQAQVFIDQL
                     VAEIEAKKEPIFAEYDARLAAAAHPNLDGDMLDHFYDSGLFAHAFNIAATAIANHASV
                     LPVGDDPIGVGPASFDASYTPAWMQAGACDYLQPIVFPLGIDVRGALSVRTDTDDYPA
                     QVDVDSPIECHAGSLGAFADLPTVTSCAIVKIAALLGDPLGSLSRAYPPNLAEQPATC
                     ISITVPGLPAPPPVEDTDTDAGTDGTDAGTDAGTGGDGTGNNDTGETPTTGGETPTTA
                     GPTSATAGDTTGDTAGTDGDSGCGCNTSTPDPTGLALGLFGLLGLRRRRRAATLAVAA
                     TLGLGACGDSGGMTTDDASTGAPTTMTTATTTTATTTDPGTTGDVDPTTTDGSASGTS
                     TGDDSTTDPTTGDDTSTTEDPTGASQAGELLDALNGTTWRGEQTRAGKTRAYELRFDS
                     DSLLWSELRNPFGPARNREMRSFKVEADGLTAHSTVIQPQGWPVNPENGRKDDWTIEV
                     IDGDPRILRTVRDGAVEEFTEGSYEAPTDGLTAIVRVFKVGGVVDKAFCDSGPNGFEY
                     PTIFDFARGKSDEIVATDFVAGARLLKWTDPSNNNQFSINDIDGFDRLGGTELSDTFN
                     FFVTYTGTIAHPGGTLSMRENDDSVEDAVWTFLGDKAGNGGENDIFFEVQGFAWPDAS
                     SDEPDADFGPGELPIEAIVVRCTESIKDVDVEIQLPNSQWSLVGNVQSTPPIDPKLFP
                     PAL"
     gene            complement(3609..>4552)
                     /locus_tag="VGB85_21540"
     CDS             complement(3609..>4552)
                     /locus_tag="VGB85_21540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136687.1"
                     /translation="AVETKPVETKAVEMKPVHEAKAETKPVEMKAVETKAVETKAETK
                     PVEMKAVETKAVEAKPLAETRPVETTPVVETKSAVEAKPVETKPVVETKPAVATKPVE
                     STPVTETKPELRPVTETKPAPAPAPAPAPAPVSQARPAVASSEPRSVPPARPDVMRVP
                     APPAGAALFGSLPSGAPRPSSGPVLPPAPALGLPRGATSAPVPKAMPTMSAPPSVASA
                     SPLRPPVVPSSSAAPWAGTPPPTLTRNLPPVAGAPVLPPRPEAVPLPRAPGAIVPTGP
                     SPVAPAAAPTLAQALPDTDDGWDDMLDALEADAPGPR"
BASE COUNT          752 a         1720 c         1446 g          634 t
ORIGIN      
        1 gcccgtgacg ctcgcccgcg tgatatgagg gtcgtctgcc atgacttgtg tacgccctgc
       61 aatcgccatt ggactcgccg cgctgctgtg catgccctcc ccggcgtacg ccttctcgac
      121 gcggatccac atcatgatcg ccaacaaggt ccgcgagtcg ctcgtcgaca gcggcaacgg
      181 caagatcgcg ctcaggctgg gtgagcactc cgtcacgctg accgcggccg acatcgaggc
      241 gctgagcgat catcccctgt cctttcgggc aggcgccgtc gggcccgaca acatggtctt
      301 cccgggcatg accgacccct cgcacgcgct cggacagcgc cccttcgagc agtgcgagct
      361 gctctaccaa gccgcggagt tgcccgagga gcgggcctac gcgctcggct gcttcctgca
      421 cggctcgacc gacgccgtcg cgcaccacta cgtcaattac atgaccggcg agaccttcac
      481 gctgacgccg atcaccagca accgcgagtc gagcttcgac aacgtcgtcc gccacatcct
      541 cgccgagtcg cagatccagg ccgccgcgat cgcgcaggac ccgggcgcct tcaccgacag
      601 ccgcctgctg cacagcatcc cgatcggctt cgtgctgcgc gcctacctcg atcaggacag
      661 ccccctgtgg cagatgatgg ccgcccaccc gaaggccgag tacgacaagg tcctcgccga
      721 ggaccccgag gcctcgctgc cgacgatcgt cgccaagatg gaggtcggcc ccgccgacca
      781 cctcgtgctc tcgccggtgt acctcgcctc cgtcgacgac ctgatcgtga agaagaagat
      841 cgagctgatg gaccgcatcg ccacgatgca ggactggaac agccccgagg gctcccagct
      901 caaggtcacc gccggcgacg acggcacgat cggcaccaag gacgacgaga ccgactgctc
      961 cgcctcctgc cccgagctgt tcgccaccta cttcaccttc gtcggcctgc tcgccccgcg
     1021 ctacaacgcc cagaaccagg agctgccgag cgccttcgag aaggtcagcg agcagctgcg
     1081 caaggagctg ttcctgtttc acgaggccta cctcgaggtc gtcgccggcc tgtccgccaa
     1141 gctcaacgcc gcgccgaacg agatgagcgg cgagttcggc gtcaacaagg aggagctcaa
     1201 ggtcctgttc cagccgctca acgactgggc cgacaagctg tcgacgatcg actacgacac
     1261 gctcgtctac gcgacggtcc ccgagtggtt catcgagctc gacaccttca tgcaggcctt
     1321 cggcgtcgac ttcgacatcg ccgcgctgat caaggccgtg ttcgacccga tcatccagcc
     1381 gatcaaggac gcgatcaagc aggccttcat cgagcaggcc caggtcttca tcgaccagct
     1441 cgtcgccgag atcgaagcca agaaggagcc gatcttcgcc gagtacgacg cccgcctcgc
     1501 cgccgccgcc cacccgaacc tcgacggcga catgctcgac catttttatg acagcggcct
     1561 atttgcccac gccttcaaca tcgccgccac cgcgatcgcc aaccacgcgt cggtgctgcc
     1621 ggtcggtgac gacccgatcg gcgtcggccc ggcctccttc gacgcctcgt acacccccgc
     1681 gtggatgcag gccggcgcct gcgactacct gcagccgatc gtgttcccgc tcggcatcga
     1741 cgtccgcggc gcgctttcgg tccgcaccga caccgacgat tacccggccc aggtcgacgt
     1801 cgacagcccg atcgaatgcc acgccggatc tctcggcgcc ttcgccgacc tgcccaccgt
     1861 caccagctgc gcgatcgtca agatcgccgc gctgctcggc gacccgctcg gatcgctcag
     1921 ccgcgcgtac ccgccgaacc tcgccgaaca gcccgccacc tgcatctcga tcaccgtgcc
     1981 cggcctgccc gcgcccccgc cggtcgagga caccgatacc gacgccggca ccgatggcac
     2041 tgacgccggc accgacgccg gcaccggcgg cgatgggacc ggcaataacg acaccggcga
     2101 gacgccaacc actggcggcg agaccccgac caccgcgggc ccgacctcgg cgaccgccgg
     2161 tgacacgacc ggcgacaccg ccggcaccga cggcgacagc ggctgcggct gcaacaccag
     2221 cacacccgac cccaccggcc tcgcgctcgg cctgttcggc ctgctcggcc tgcgtcgccg
     2281 ccggcgcgcc gcgacgctcg ccgtcgcggc gacgctcggc ctcggcgcct gtggtgacag
     2341 cggcggcatg accaccgatg acgccagcac gggcgcgccg accaccatga cgaccgcgac
     2401 caccacgacc gcgacgacga ccgacccggg cacgaccggc gacgtcgacc cgacgacgac
     2461 cgacggcagc gcgagcggca cgagcacggg cgacgactcg acgaccgacc cgacgaccgg
     2521 cgacgacacg agtacgaccg aggacccgac cggcgcctcg caggcgggcg agctgctgga
     2581 cgcgctcaac ggcaccacct ggcgcggcga gcagacacgc gccggcaaga cccgcgcata
     2641 cgagctgcgc ttcgacagtg attcgctgtt gtggtccgag ctgcgcaacc ccttcggccc
     2701 ggcgcgcaat cgcgagatgc gcagcttcaa ggtcgaggcc gacggcctga ccgcgcactc
     2761 gaccgtcatc cagccgcagg gctggccggt caacccggag aacggccgca aggacgactg
     2821 gacgatcgag gtgatcgacg gcgacccgag gatcctccgg accgtgcgcg acggcgccgt
     2881 cgaggagttc acggagggca gctacgaggc gccgaccgac ggcctgacgg cgatcgtccg
     2941 cgtattcaag gtcggcggcg tcgtcgacaa ggcgttctgc gactcgggcc ccaacggctt
     3001 cgagtacccg acgatcttcg acttcgcccg gggcaagtcc gacgagatcg tcgccaccga
     3061 cttcgtcgcc ggcgccaggc tgctcaagtg gaccgacccg agcaacaaca accagttctc
     3121 gatcaacgac atcgacggct ttgaccgcct cggcggcacc gagctgagcg acacttttaa
     3181 ttttttcgtc acctacaccg gcacgatcgc ccacccgggc ggcacgctgt cgatgcgcga
     3241 gaacgacgac agcgtcgagg acgcggtgtg gaccttcctc ggcgacaagg ccggcaacgg
     3301 cggcgagaat gacatctttt tcgaggtgca gggcttcgcc tggcccgacg cctcgtccga
     3361 cgagcccgac gccgacttcg gccccggcga gctcccgatc gaagcgatcg tggtccgctg
     3421 caccgagtcg atcaaggacg tcgacgtcga gatccagctc ccgaacagcc agtggtcgct
     3481 cgtcggcaat gtgcagagca ccccgccgat cgacccgaag ctgttcccgc cggcgctgtg
     3541 aaggcttttt tttaataaaa tgagaagtgc ccgtccgctc gacgcggacg ggcactggcg
     3601 cgtgcaggtc agcgcgggcc cggagcgtcg gcctcgagcg cgtcgagcat gtcgtcccag
     3661 ccgtcgtccg tgtcgggcaa cgcctgcgcg agcgtcggcg cggccgcagg cgcgaccggg
     3721 ctcggccccg tcggcacgat cgcaccgggc gcgcgtggca gcgggaccgc ctcgggacgc
     3781 ggcggcaaca ccggcgcacc cgcgaccggc ggcaggttgc gcgtgagggt cggcggcggc
     3841 gtcccggccc acggcgcagc gctcgacgag ggcacgaccg gcggacgcag cggcgaggcg
     3901 ctggcgaccg acgggggcgc agacatcgtc ggcatggcct tcgggacagg tgcagaggtc
     3961 gccccgcgcg gcagaccgag cgccggcgca ggcggcaata ccggcccgga cgacggccgc
     4021 ggggcccccg acggcagcga accaaacagc gcagccccgg ccggcggcgc cggcacgcgc
     4081 atcacatcgg ggcgcgctgg cgggaccgaa cgcggctccg acgacgcgac cgcgggcctg
     4141 gcctgagaga caggtgcggg cgcgggtgcg ggtgcaggtg cgggcgcggg ctttgtctcg
     4201 gtgacaggcc tcagctcggg cttcgtctcg gtgaccggcg tcgactcgac aggcttcgtt
     4261 gcgaccgcgg gcttcgtctc gacgactggc tttgtctcaa ccggcttcgc ctcgaccgca
     4321 gacttcgtct cgaccaccgg cgtcgtctcg accggccttg tctcggcgag cggcttcgcc
     4381 tcgacggcct tcgtctcgac ggccttcatc tcgaccggct tcgtctcggc cttcgtctcg
     4441 acagccttcg tctcgacggc cttcatctcg accggcttcg tctcggcctt cgcctcgtga
     4501 accggcttca tctcgacagc cttcgtctcg accggcttcg tctcgacagc ct
//
LOCUS       DASWNJ010001286         3213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_128059, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001286 DASWNJ010000000
VERSION     DASWNJ010001286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128059"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..1301
                     /locus_tag="VGB85_21545"
     CDS             27..1301
                     /locus_tag="VGB85_21545"
                     /inference="COORDINATES: protein motif:HMM:NF013333.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0010142 - farnesyl diphosphate biosynthetic
                     process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEY0136688.1"
                     /translation="MTAMTRPERVGIERIAAYPCTLSLDMRELAQARDHDPRHPVEEL
                     WVTSRSINPPWEDPVTMAVNAALRVVTPQDREDIELVIVGTESSPDYGKPMSTYVQRW
                     CQIQPNCRNFEAKHACYGGTSGLMMAAHWVASGASPGKKALVVTSDQSRTHLGQPWEY
                     VLGAAATAMIVSAQPDVLEIELDKTGYWTSEIFDTYRPTSQKEVGHADTSLYGYLEAL
                     EGAYDHFLRKVGPIDYDTFFKKHVYHVPFGGMTFRAHRALLRRWTAHTGGADLGVKAA
                     REHFERKSKPALKYNAQFGGTYTSATFIALMGLIDSFDDLDPGDRVSMFSYGSGSCAE
                     FYSGKVGPHARARVAEAALQAGLDARHKLTVAEYEAVERARSEQIDRSDVTVDHAGAS
                     CSLEGLYDRVYAGKGRLVLDGVTEYQRNYRLS"
     gene            1436..2227
                     /locus_tag="VGB85_21550"
     CDS             1436..2227
                     /locus_tag="VGB85_21550"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY0136689.1"
                     /translation="MYLRFEDLGSPVWRVYLTPDEHGEVAIGGPGVADLEELLQRAEQ
                     DPGCRVIVIASEGGAEFCRGMDLRGLSSGSVASRAEGVARYARCLGLMRGLRAAVVCL
                     VDGAVSGGGVGLVAACDLAIVGPAASFVLTELYFGLVPAVILPVLGERMGPARARWLA
                     LSGVTLNGDTAVHLGLADERSDDFTRTLAVRLKHLLRLNPEAVGRLKRLSAEITGQPL
                     ELALAAGVARTTIDIDGPEVAAAVESYLAGELPPWAIRQRPEGQR"
     gene            2224..3003
                     /locus_tag="VGB85_21555"
     CDS             2224..3003
                     /locus_tag="VGB85_21555"
                     /inference="COORDINATES: protein motif:HMM:NF005496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase"
                     /protein_id="HEY0136690.1"
                     /translation="MTGPVPTDSLELTVDGEGLATLRMRDEAGKNAFSEPFVAALVQT
                     LTAIGGRADIKVVVLCGLPDVFSAGGDRSVLLGLAEGRIAPYELLLTRTLLELPQPSI
                     AAIAGAAVGGGLVFGLACDLVFMARESRYGCNFMDLGFTPGMGTTRLLQAAVGPYVAA
                     EMMLSAQYFRGADLASRGAQVNGVLPRVEVEARALDVAGRLCEKPRAALLLLKRSLGL
                     PRRLAFEEARTVESMMHEICFADPHTQATIRENYRENYLKP"
     gene            3000..>3213
                     /locus_tag="VGB85_21560"
     CDS             3000..>3213
                     /locus_tag="VGB85_21560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0136691.1"
                     /translation="MTANFNDFTGKAVLITGGTRGIGLACGLAFARHGARVILTHRWG
                     SADEGELRAKFAELGAPEPMIVEADVS"
BASE COUNT          463 a         1162 c         1122 g          466 t
ORIGIN      
        1 cggcctcgac tggacctact gacgccatga ccgcgatgac cagacccgag cgcgtcggca
       61 tcgagcggat cgccgcgtac ccgtgcacgc tgtcgctcga catgcgcgag ctggcccagg
      121 cccgcgatca cgacccgcgg catccggtcg aggagctgtg ggtcacctcg cgctcgatca
      181 acccgccgtg ggaagacccg gtgacgatgg ccgtcaacgc ggccctgcgc gtcgtcaccc
      241 cgcaggaccg cgaggacatc gagctcgtga tcgtcggcac cgagtcctcg ccggactacg
      301 gcaagccgat gtcgacctac gtgcagcgct ggtgccagat ccagcccaac tgccgcaact
      361 tcgaggccaa gcacgcctgc tacggcggca cctcgggcct gatgatggcc gcgcactggg
      421 tcgcctccgg ggcgtcccca ggcaagaagg cgttggtcgt caccagcgat cagagccgca
      481 cgcacctcgg gcaaccgtgg gaatatgtgc tcggcgcggc cgcgacggcg atgatcgtca
      541 gcgcccagcc cgacgtgctg gagatcgagc tcgacaagac cggctactgg acgagcgaga
      601 tcttcgacac ctaccgcccg acctcgcaaa aggaggtcgg ccacgccgac accagcctgt
      661 acggctacct cgaggcgctc gagggcgcgt acgaccactt cctgcgcaaa gtcggcccga
      721 tcgactacga caccttcttc aagaagcacg tctaccacgt gcccttcggc ggcatgacct
      781 tccgggccca ccgcgccctg ctgcgccgct ggaccgcgca cacgggcggc gccgacctcg
      841 gcgtcaaggc ggcccgcgag cacttcgagc gcaagtcgaa gccggccctc aaatacaacg
      901 cgcagttcgg cggcacgtac acctcggcga ccttcatcgc cctgatgggc ctgatcgaca
      961 gcttcgacga cctcgacccc ggcgaccgcg tgtccatgtt ctcgtacggc tccggctcct
     1021 gcgcggagtt ctactccggc aaggtcggcc cgcacgcgcg ggcgcgcgtc gccgaggcgg
     1081 cgctgcaggc cgggctcgac gcccgccaca agcttacggt cgccgagtac gaggcggtcg
     1141 agcgcgcccg cagcgagcag atcgatcgca gcgacgtcac ggtcgaccac gcgggcgcgt
     1201 cgtgcagcct cgagggcctg tacgaccgcg tgtacgccgg caaggggcgc ctcgtgctcg
     1261 acggcgtcac cgagtatcag cgcaactatc gcctcagcta ggctcgtcgg gtcgtccatg
     1321 cacggtgacc tcggcacgcg acttcttcgg caccacgccc gcgctccccg ggagcgcacg
     1381 gccggcgcct cgcctgtcgt gaacgcgaga agcgtcgcgc ccctggaggt ctgtcatgta
     1441 cctgcgattt gaagaccttg gttcgcccgt gtggcgcgtg tacctgacgc ccgacgagca
     1501 cggcgaggtc gcgatcggcg ggcccggggt cgcggacctc gaggagctgt tgcagcgcgc
     1561 cgagcaggac ccgggctgtc gcgtgatcgt gatcgccagc gagggcgggg cggagttctg
     1621 tcgcggcatg gacctgcgcg ggctcagcag cggcagcgtc gcctcgcggg ccgagggcgt
     1681 cgctcgctac gcgcgctgcc tcggtctgat gcgcggcctg cgggccgcgg tcgtgtgcct
     1741 ggtcgacggg gcggtgtcgg gcggcggggt cggcctggtc gccgcgtgcg acctggcgat
     1801 cgtcggaccg gcggccagct tcgtgctgac cgagctgtac ttcgggctcg tgcccgcggt
     1861 gatcttgccg gtgctcggcg agcgcatggg gccggcgcgc gcgcgctggc tcgcgctctc
     1921 cggggtcacc ctcaatggcg acacggcggt gcacctcggc ctcgccgacg agcgcagcga
     1981 cgacttcacg cgcacgctgg cggtccgcct caagcacctg ctgcgcctca acccggaggc
     2041 ggtcgggcgg ctcaagcgac tgtcggcgga gatcaccggg cagccgctgg agctcgcgct
     2101 cgcggccggg gtcgcgcgca cgacgatcga catcgacggc cccgaggtcg ccgccgcggt
     2161 cgagagctac ctcgccggtg agctcccgcc gtgggcgatc cgccagcgcc cggagggcca
     2221 gcgatgaccg gacctgtccc aacggacagc ctcgagctga cggtcgacgg cgagggcctg
     2281 gcgaccctgc gcatgcgcga cgaggccggc aagaacgcgt ttagcgagcc cttcgtcgcc
     2341 gcgctggtgc agacgctgac cgcgatcggc ggccgcgcgg acatcaaggt cgtcgtactc
     2401 tgcggcctgc cggacgtgtt ctctgccggc ggcgaccgca gcgtgctgct cggcctcgcc
     2461 gagggacgga tcgcccccta cgagctcctg ctgacgcgga ccctgttgga gctcccgcag
     2521 ccgagcatcg ccgcgatcgc gggcgccgcg gtcggtggcg gactggtgtt cgggctcgcc
     2581 tgcgatctcg tgttcatggc ccgtgagagt cgctacggct gtaacttcat ggacctgggg
     2641 ttcacccccg gcatgggcac cacgcggctc ctgcaggccg ctgtgggccc ctacgtggcc
     2701 gccgagatga tgctgagcgc gcagtacttt cgcggcgccg atctcgcgag ccgcggcgca
     2761 caggtcaacg gtgtgttgcc ccgcgtcgag gtcgaggcgc gggcgctgga cgtcgccggt
     2821 cgcctgtgcg aaaagccccg cgcggcgctg ctgctgctca agcgcagcct cggtctgccg
     2881 cggcgcctgg ccttcgagga ggcccgcacc gtcgagtcga tgatgcacga gatctgcttc
     2941 gcggaccccc acacccaggc gactatccga gagaattacc gcgagaatta cttgaagcca
     3001 tgaccgcgaa tttcaacgac ttcaccggga aggcagtgct gatcaccggc ggcacccgcg
     3061 gcatcggcct cgcctgcggc ctcgcgttcg cccgccacgg ggcccgcgtg atcctgacgc
     3121 accgctgggg cagcgccgac gagggcgagc tccgggccaa gttcgccgag ctcggcgcgc
     3181 ccgagccgat gatcgtcgag gccgacgtct cgc
//
LOCUS       DASWNJ010001287         2153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_588221, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001287 DASWNJ010000000
VERSION     DASWNJ010001287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588221"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="VGB85_21565"
     CDS             <1..951
                     /locus_tag="VGB85_21565"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0136692.1"
                     /translation="CNIGGVLPGTGPRRIVVGAHVDHLGLGTGSSLAPGQRAVHNGAD
                     DNASGVATMLALAAALAGVPAASRPYTVEFVAFGAEEMGLLGSKHYVAGLSPDARAAL
                     VAMVNFDMVGRLQGDAVVVSGVGTSSLWKGLLAEVAAPLEVRPSEDGFGASDQASFYA
                     AGLPVLHFCIGTHADYHKPSDDLDKINFPGAAAIGDLSLRVLGRLMRDQPNLDFIKVA
                     TPTPRAGGFRVSLGTVPDYGAKADGVALADVRPGGPAAAAGLKAGDVITLLGTREIHS
                     LDDYMASFAELQPGVEVPVRVLRNGAAVDLRITPAAPQRR"
     gene            1124..>2153
                     /locus_tag="VGB85_21570"
     CDS             1124..>2153
                     /locus_tag="VGB85_21570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136693.1"
                     /translation="MQRIWTTLVDGFDVPLLGASLLALGACSLPGCVFNGSGAGTTEV
                     ALDTGDLTTEASGAESSTGVSDPGDSGDTSAAADTSTSAADDETSSSSGGPPDPETSS
                     TTGTDTGEDLCGNGKLDPGEGCDGELGPESCAEIDAAYTEGAPTCDACQLDISACVTC
                     QAPNIKPCDDGSDEPLHALELGCDSMNGWSPADAVHLENEKLAALDATSYRVVRRFGS
                     HPDAWLPHAGSKALLIGTGSFQMADDNGVVTDKAGDAQYGGSNLNSDSMGMFPDIPGV
                     QPVNGGSAPFVDCDGEHDCSNTLSGQWNALSPKDARDVFWLTVDTVVPPGTHGFALDL
                     AFFTAHYPE"
BASE COUNT          312 a          835 c          687 g          319 t
ORIGIN      
        1 tgcaacatcg gcggcgtgct gcccggcacc ggcccgcggc ggatcgtcgt cggcgcgcac
       61 gtcgaccatc tcgggctcgg caccggcagc tcgctcgcgc cggggcagcg ggccgtccac
      121 aacggcgccg acgacaacgc ctccggcgtc gccaccatgc tcgccctcgc cgccgcgctc
      181 gccggggtcc cggccgcctc gcgcccctac accgtcgagt tcgtcgcctt cggcgccgag
      241 gagatgggcc tgctcggctc gaagcactac gtcgccggtc tctcgccgga cgcccgcgcc
      301 gcgctggtcg ccatggtgaa cttcgacatg gtcggccggc tacagggtga cgccgtcgtc
      361 gtttcggggg tcgggacctc ctccttatgg aagggtctgc tcgccgaggt ggcggccccc
      421 ctcgaggttc gcccgagcga ggacggcttt ggcgcctcgg atcaggccag cttttatgca
      481 gccggtctgc cggtcctaca tttctgtatc ggcacccacg ccgactatca caagcccagc
      541 gacgacctcg acaagatcaa cttccctggc gccgccgcga tcggtgatct ctccctgcgt
      601 gtgctcgggc gcctcatgcg cgaccagccg aacctcgact ttatcaaggt cgcgacgccc
      661 acgccgcggg ctggcggctt tcgtgtgtcg ctggggaccg tgccggatta cggggccaag
      721 gccgacggcg tcgccctcgc ggatgtccgt cccgggggtc cggccgccgc cgcggggctc
      781 aaggcgggtg acgtgatcac cttgctcgga acgcgggaga tccacagcct cgacgactat
      841 atggccagtt tcgcggagtt gcagccaggc gtcgaggtgc cggtccgggt cctgcgaaac
      901 ggcgccgccg tcgatctgcg gatcacaccc gccgcaccgc agcgacgcta ggcgtcaccc
      961 cggcgtcacc ccggagtcgc tggatccacg gtctccccga ccccggcgct tccttgccac
     1021 ccggtgtcgc ggcgcacccc agccgcagtt ggaacgtcgc gccccgcttg accgccagat
     1081 cccacctttg tatgaccaag atatcggttt gcggaggctc gacatgcaaa ggatctggac
     1141 gaccctggtt gacggtttcg acgtccccct tctcggcgcc tcgttgctgg ccctcggggc
     1201 gtgcagcctc ccgggctgcg tcttcaacgg gagcggcgcc ggcaccacgg aggtcgccct
     1261 cgataccggc gacctgacca ccgaggcctc cggcgccgag tccagcaccg gcgtgtccga
     1321 tcccggtgat tccggtgata ccagcgccgc cgcggatacc tccacctcgg ccgccgacga
     1381 cgagaccagc agctcctccg gcgggccgcc cgaccccgag accagcagta ccaccggtac
     1441 cgacaccggg gaggacctgt gcggcaacgg caagctcgac cccggcgagg gctgcgacgg
     1501 cgagctgggc cccgagtcct gcgccgagat cgacgccgcc tacaccgagg gcgcgccgac
     1561 ctgcgatgcc tgtcagctcg acatcagcgc gtgtgtcacc tgccaggcgc cgaacatcaa
     1621 gccctgcgac gacggcagcg acgagccgct gcacgccctc gagctcggct gcgacagcat
     1681 gaacggctgg tcccccgccg acgccgtcca cctcgagaac gagaagctcg ccgcgctcga
     1741 cgcgacctcg taccgcgtcg tccgccgctt cggcagccac cccgacgcct ggctcccgca
     1801 cgccggcagc aaggccctcc tgatcggcac cggcagcttc cagatggcgg acgacaacgg
     1861 cgtcgtcacc gacaaggccg gcgacgccca gtacggcggc tccaacctca actccgacag
     1921 catgggcatg ttccccgaca tcccgggcgt gcagccggtc aacggcggca gcgccccctt
     1981 cgtcgactgc gacggcgagc acgactgctc gaacaccctc tcgggtcagt ggaacgcgct
     2041 gtcgcccaag gacgcgaggg acgtcttctg gctcacggtc gacaccgtcg tgccgcccgg
     2101 cacccacggc ttcgccctcg acctcgcgtt tttcaccgcc cactaccccg agt
//
LOCUS       DASWNJ010001288         3060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_515762, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001288 DASWNJ010000000
VERSION     DASWNJ010001288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515762"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VGB85_21575"
     CDS             complement(<1..756)
                     /locus_tag="VGB85_21575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136694.1"
                     /translation="MMAAGFLAADKAPAIDVSAYLPTATGVLWTVLALALLFFAVRPE
                     LWRRLWFQRVDPRPAGLLRIVFGIAVLWTIVDLLPLARFLFTDEGLWLPKMARRNYGG
                     DFRTIWDPEHGFEHWYDVLLAFWYRFSLFHIRADPAFVYTIYAITIAALTLMILGIKT
                     RISTIVSWLLVNSIYVYSPIFYSGGDTVVRVFLFLGMLSGWGEAYSLDAWWRRKRAVL
                     AGAAKIPPLRPIAAWPLRLMMLQLAIIYCATGAL"
     gene            complement(753..1685)
                     /locus_tag="VGB85_21580"
     CDS             complement(753..1685)
                     /locus_tag="VGB85_21580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136695.1"
                     /translation="MTTAPDRPPSHPSHPSHPSDTASDHPTDSLAESLLRAPLEVRAE
                     DGRRWAYRPVGLVLVSLFVLYHVVALLQHTTPGGGLAANFSRRLAEVLHIGSYMRATS
                     NVQSWSMFAPNPHRSNMFLQVFVELADGTILDLEHDMYGRRAYPYVFYDRMGKINRRL
                     LEQDRYQRHYAAWVCRDWALHHGGEMPVRVKYVKMWTKVPHPAKVIPNWGFDPMELKL
                     TKEHLPSIECATTVHAQLPDDIRARHGLPPLRPNEFRDAVVQTWWDKAEMRAKTIERR
                     RALTPGEDSERDEKVVEPPAAETPPPAAGEGGGD"
     gene            complement(1699..>3060)
                     /locus_tag="VGB85_21585"
     CDS             complement(1699..>3060)
                     /locus_tag="VGB85_21585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136696.1"
                     /translation="LRRMHLALAAVIPIVAALWFVLARWPARIRRWKFDQVSLRRWLF
                     GRRVWLGLGLMFHGFLILFMNIGMFPFIMLMTYAAWLTGEEFAAALRWCVETVQRSPL
                     GRALPARVFERMLTRAGPAEAPEDVPPRGRPIPDLLVVLLGLGLLYLLWRRIDGDREL
                     TTLVYCWAGLVLAVGVTCRLWRNRPPSGAPALAYGPAGRALALAFMLSHALAVGLSLG
                     PNYNIFASWRGPARSFFGAWPTVTGTSQSWKMFAPNPPRSNVFMKTVVVTQNGERWNI
                     GNNAYDYRPFPWVWNDRMRKMQRRMVSKGKSYLRNWANFHCREWYLQTGQRAASVEIH
                     KLSTRIPSPEQVATKGYYDPTRLKLNDELVETYACPRAGDILPFMKLRRGWPLSAEEQ
                     AQLAEEAEREAKAAENKRKSWASRRDWGGSGPPPLTYRATPPPVAPADDDTPAAKAGD
                     DGE"
BASE COUNT          485 a          975 c         1161 g          439 t
ORIGIN      
        1 gagggcgccg gtggcacagt agatgatcgc cagctgcagc atcatcaggc gtagcggcca
       61 cgcggcgatc gggcgcagcg gggggatctt cgcggcgccg gcgagcacgg cccgcttgcg
      121 gcgccaccag gcgtcgagcg agtaggcctc gccccagccg ctgagcatgc ccaggaacag
      181 gaacacgcgc acgacggtgt cgccgccgct gtaaaagatc gggctgtata cgtagatcga
      241 gttgacgagc agccagctga cgatcgtgct gatgcgggtc ttgatcccga ggatcatcag
      301 cgtcagcgcg gcgatcgtga tcgcgtagat cgtgtagacg aaggccgggt cggcgcggat
      361 gtgaaacagc gagaagcggt accaaaacgc cagcaagacg tcgtaccagt gctcgaagcc
      421 gtgctccggg tcccagatcg tgcgaaagtc gccgccgtag ttgcggcgcg ccatcttcgg
      481 cagccacagg ccctcgtcgg tgaacaggaa gcgggccagc ggcagcaggt cgacgatcgt
      541 ccacagcacc gcgatgccga acacgatccg cagcaggccc gcggggcgcg ggtcgacgcg
      601 ctggaaccac aggcggcgcc acagctccgg gcgcacggcg aagaacagca gcgcgagcgc
      661 gagcacggtc cacagcacgc cggtggcggt cggcaggtag gccgagacgt cgatcgcggg
      721 ggccttgtcg gcggcgagga atccggcggc catcaatcgc cacctccttc accggcggcg
      781 ggcggaggtg tctcggcggc cggcggctcg accaccttct catctcgctc gctgtcctcg
      841 ccgggcgtga gggcccggcg gcgttcgatc gtcttggcgc gcatctcggc cttgtcccac
      901 caggtctgga cgacggcgtc gcggaactcg ttcgggcgca gcggtggcag gccgtggcgg
      961 gcgcggatgt cgtcggggag ctgcgcgtgc acggtggtcg cacactcgat gctcggcagg
     1021 tgctccttgg tcagcttgag ctccatcggg tcgaagcccc agttcgggat caccttggcg
     1081 gggtgcggga ccttggtcca catcttgacg tacttgaccc gcaccggcat ctccccacca
     1141 tggtggagcg cccagtcgcg gcacacccac gcggcgtagt gcctctggta tctgtcctgc
     1201 tcgagcaggc ggcggttgat cttgcccatg cgatcgtaaa agacatacgg gtaggcccgc
     1261 cggccgtaca tgtcgtgctc gaggtcgagg atcgtgccgt ccgcgagctc gacgaagacc
     1321 tgcaagaaca tgttgctgcg gtgcgggttg ggggcgaaca tcgaccagct ctgcacgttc
     1381 gaggtcgcac gcatgtacga accgatgtgc agcacctcgg cgagccggcg gctgaagttg
     1441 gccgccaaac caccaccggg ggtcgtgtgc tgcagcaacg cgaccacgtg gtaaagcacg
     1501 aacaggctga cgagcacgag gccgaccgga cggtaggccc agcggcgacc gtcctcggcg
     1561 cggacctcga gcggggcgcg tagcagcgac tcggcgagcg agtcggtagg gtggtcgctc
     1621 gcggtgtccg acggatgcga cggatgcgac ggatgcgacg gaggtcggtc gggtgcggtc
     1681 gtcacgggtc ctttgcggct actcgccgtc gtcgccggcc ttggcggcgg gggtgtcgtc
     1741 gtcggcgggg gcgacggggg gcggggtggc gcggtacgta aggggcggcg gtccgctgcc
     1801 gccccagtcg cggcggctgg cccagctctt gcgcttgttc tcggcggcct tggcctcgcg
     1861 ctcggcctcc tcggcgagct gggcctgctc ctccgcggag agcggccagc cgcggcgcag
     1921 cttcatgaac ggcaagatgt cgccggcgcg cgggcaggcg taggtctcga ccagctcgtc
     1981 gttgagcttg aggcgggtgg ggtcgtagta gccctttgtg gcgacctgct cggggctcgg
     2041 gatgcgggtc gagagcttgt ggatctcgac ggacgcggcg cgttgaccgg tctgcaggta
     2101 ccactcgcgg cagtgaaagt tggcccagtt gcgcaggtag ctcttgccct tgctgaccat
     2161 gcgacgctgc atcttgcgca tgcggtcgtt ccagacccac gggaacggcc ggtagtcgta
     2221 ggcgttgttg ccgatgttcc agcgctcgcc gttttgcgtg acgacgacgg tcttcatgaa
     2281 cacgttcgag cgcggcgggt tgggggcgaa catcttccag ctctgcgagg tgccggtgac
     2341 ggtcggccag gcgccgaaga aggagcgcgc cgggccgcgc cagctggcga agatgttgta
     2401 gttgggcccg aggctgaggc cgacggcgag cgcgtgcgag agcatgaacg cgagcgcgag
     2461 ggcgcggccg gccgggccgt aggcgagggc aggggcgccg ctgggcggtc ggttgcgcca
     2521 cagccggcag gtgacgccga ccgcgagcac gaggccggcc cagcagtaga cgagggtggt
     2581 cagctcgcgg tcgccgtcga tccggcgcca gaggaggtac agcaggccga ggccgagcag
     2641 cacgacgagc aggtcgggga tcggccggcc gcgggggggc acgtcctcgg gggcctcggc
     2701 cgggccggcg cgcgtcagca tgcgctcgaa cacccgcgca ggcagggccc ggccgagcgg
     2761 cgagcgctgc acggtctcca cgcaccagcg cagcgccgcc gcgaactcct cgccggtcag
     2821 ccacgcggcg taggtcatca gcatgatgaa ggggaacatg ccgatgttca tgaacaggat
     2881 gagaaacccg tggaacatca ggccgaggcc gagccacacg cggcggccga acagccaccg
     2941 gcgcagcgag acctggtcga acttccaccg gcggatccgg gccggccagc gcgcgagcac
     3001 gaaccacagc gccgcgacga tcgggatcac cgcggcgagg gccaggtgca tccgccgcag
//
LOCUS       DASWNJ010001289         2564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_200382, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001289 DASWNJ010000000
VERSION     DASWNJ010001289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200382"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VGB85_21590"
     CDS             complement(<1..559)
                     /locus_tag="VGB85_21590"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0136697.1"
                     /translation="MAKDIHRSSEVSRLIGAIEEAPEGLSIVGISGPGGIGKSFLLSH
                     VLETVDPTRLGYLVLTADAANPDTRGDFLGLVEGQLFRRSLPPPADRSKDYFPHLREV
                     AALHRDLVDRAVAELSRQGAPDLVRRAAVALLRNARVLNKAVPKSRLALDLTRIDDAD
                     VEKVLDQAWDTVRALPGLRDSTALWG"
     gene            705..872
                     /locus_tag="VGB85_21595"
     CDS             705..872
                     /locus_tag="VGB85_21595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136698.1"
                     /translation="MRRRSAKLPERVIISEYGPPPTSSTAFSITGDRAMKVMPKIDPC
                     EMPYQCSCCGS"
     gene            complement(917..1597)
                     /locus_tag="VGB85_21600"
     CDS             complement(917..1597)
                     /locus_tag="VGB85_21600"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEY0136699.1"
                     /translation="MPAGAVFFLKVCCIASVEEARLAVAHGAAGLGLVSAMPSGPGVI
                     DEDTIAEIVAAVPGEVATFLLTSQTTAAGIVAQQRRTGANTLQLVDAVPAEQLRALRV
                     ALPGVRLVQVIHVRDAGSLVEARSVASLVDALLLDSGDPGAVVRTLGGTGRVHDWAIS
                     RQICEAVEIPVFLAGGLRPENVADAVAAVRPAGVDVCSGLRTDGRLDADKLRRFAGAL
                     TLRAPIFA"
     gene            complement(1654..>2564)
                     /locus_tag="VGB85_21605"
     CDS             complement(1654..>2564)
                     /locus_tag="VGB85_21605"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY0136700.1"
                     /translation="PILVRWSMGFGKVEFVRALLDAGADPQKAPGALAWAAHYGHTEV
                     VRVLLAAGYPPDDCAGTTPALACAAAFARREIVAMLLAAGAKQRDVALYCAAGHSLQS
                     VGPDIRERPAERLEVLRMLLAAGANPSAHEQRFIGSTPLHVAARVGSGEIAELLLTAG
                     GAVDERDDLGRTPLWVAAEQGSARVVELLLRAGATGRTTDHAGVSAYDIARKTTSDAG
                     VVEIYPGTGNHLRVMALLREASAGPVKPVAPAPPAGPAAGDRVRHAKFGEGTIRAIEG
                     EGAKLTIDFDTSGQKVLLARFVERIV"
BASE COUNT          381 a          936 c          906 g          341 t
ORIGIN      
        1 ggccccacag cgccgtcgag tcgcgcaggc ccggcaacgc gcgcaccgtg tcccacgcct
       61 ggtcgagcac cttctcgacg tccgcgtcgt cgatccgcgt caggtcgagc gcgaggcggc
      121 tcttgggcac cgccttgttg agcacccgcg cgttgcgcag cagggcgacc gccgcgcggc
      181 gcaccaggtc cggcgcgccc tgacgcgaga gctcggcgac ggcgcggtcg acgaggtcac
      241 ggtgcagcgc ggcgacctcg cgcaggtgcg ggaagtagtc cttgctgcgg tcggccggcg
      301 gcggcagcga gcggcgaaac agctggccct cgacgagccc gaggaagtcg ccgcgggtgt
      361 ccgggttggc ggcgtcggcg gtgagcacga gataaccgag tcgggtcggg tcgacggtct
      421 cgagcacgtg cgacagcagg aagctcttgc cgatcccgcc gggcccgctg atgccgacga
      481 tcgagagccc ctcgggggcc tcctcgatcg cgccgatcag ccgcgagacc tccgagctgc
      541 ggtggatgtc cttggccatg cccgcgagag cataccccgg gacgacgttc gctgccgctc
      601 gccgccgcgc agagcgctca ctccgggtcg caagggccgt cgcagaagtt ctcgatcttg
      661 ccgcccggtt tttaaagggc gtcgatcgcg tcgcggatct tcggttgcgg cgcaggtccg
      721 cgaagctgcc cgagcgggtc atcatcagcg agtacggccc gccgccgacg tcgagcacgg
      781 ccttctcgat caccggcgac agggccatga aggtcatgcc gaagatcgac ccctgcgaga
      841 tgccgtacca gtgcagctgc tgcgggtcgt agatcggctc gccgaacgcc tggacggcct
      901 cgcagcccgg gtattttcag gcgaagatcg gcgcgcgcaa tgtcagcgcg ccggcgaagc
      961 ggcgtaattt atccgcgtcg agccggccgt cggtgcgcag cccggagcag acatcgaccc
     1021 ccgccggccg cacggcggcg accgcatcgg cgacgttctc cgggcgcagc ccgccggcca
     1081 gaaacacggg gatctccacg gcctcgcaga tctgccggga tatcgcccag tcgtggacgc
     1141 gcccggtgcc gccgagcgtg cgaaccaccg cgcccgggtc ccccgagtcc agcaacagcg
     1201 cgtcgacgag gctcgccacg ctgcgcgcct cgacgagcga accggcgtcg cgcacgtgga
     1261 tcacctggac gaggcgcaca cccggcagcg cgactcgcag cgcccgcagc tgctccgccg
     1321 gcacggcgtc gaccaattgc agggtgttgg cgcccgtgcg ccgctgctgg gcgacgatgc
     1381 cggcggccgt cgtctggctg gtgagcagga aggtcgccac ctcccccggc acggcggcga
     1441 cgatctccgc gatcgtatcc tcgtcgatca cgcccggccc gctcggcatc gccgacacca
     1501 gaccgagccc cgcggcccca tgcgccaccg cgagccgcgc ctcctccacg gaagcaatac
     1561 agcacacttt taaaaaaaag acggcacccg caggcatggc cgagggagtt aacacggcag
     1621 caggggcgct cgcaagtgtg gtgatatcga cgattacacg atgcgctcga cgaaccgcgc
     1681 cagcagcacc ttctggcccg aggtgtcgaa gtcgatcgtg agcttggcgc cctcgccctc
     1741 gatcgcgcgg atcgtgccct cgccgaattt cgcgtggcgc acgcggtcgc ccgcggccgg
     1801 gccggcgggc ggcgccggtg cgaccggctt gaccgggccg gccgaggcct cgcgcagcag
     1861 cgccatcacc cgcaagtgat tgccggtgcc gggatagatc tcgacgacgc cggcgtcgct
     1921 ggtcgtcttc ctggcgatgt cgtacgccga tacgccggcg tggtcggtgg tcctcccggt
     1981 cgcgcccgcg cgcagcagca gctcgacgac gcgggcgctg ccttgctcgg ccgcgaccca
     2041 cagcggcgtg cggccgaggt cgtccctctc gtcgaccgcg ccgcccgcgg tgagcagcag
     2101 ctcggcaatt tctcccgatc ccacgcgcgc cgcgacgtgc agcggcgtcg agccgatgaa
     2161 ccgctgctcg tgcgcggacg ggttcgcgcc ggccgcgagc aacattcgta gcacctcgag
     2221 gcgctcggcc ggacgttcgc ggatatcggg gccgaccgac tgcaatgaat gtcccgcggc
     2281 gcagtacaac gcgacatctc gctgcttcgc cccggccgcg agcaacatcg ccacgatctc
     2341 gcggcgcgcg aaggcggccg cgcaggcgag cgcgggcgtc gtgccggcgc aatcgtccgg
     2401 cggataaccg gccgcgagca gcacgcggac gacctccgtg tggccgtagt gcgcggccca
     2461 ggcgagcgcg ccgggggcct tttgcgggtc ggcgccggcg tcgagcaagg cgcgcacgaa
     2521 ctcgaccttg ccaaagccca tcgaccagcg gacgaggatc gggc
//
LOCUS       DASWNJ010001290         5441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_515859, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001290 DASWNJ010000000
VERSION     DASWNJ010001290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515859"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="VGB85_21610"
     CDS             <1..944
                     /locus_tag="VGB85_21610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0136701.1"
                     /translation="TGEPYLCGDVQSDERELIGVREGLGVQSSIAVVIVVGERRGVLS
                     IASLQPQRFDEADLRFVETVARWLGILIHRTELVERVAQEAASQARKIAAEEIVTIVA
                     HDLRNHLAAIHGRLHLIGARSRKDGRAQDQGDADQALRVLARLRSMVSDMLDLSRLGR
                     GSLVVCPEPLDLVMLVQETVDLLRAPNLEITITGPESLVVGGDPLRLRQVLENLLGNA
                     IKHSPTGAPVFVMLAKQARGADEFAVVSVKDSGPGVDPRILPRLFDRFTTGPGSSGLG
                     LGLYIARGITEAHGGTLTVESAPGQGATFSLALPLTA"
     gene            complement(934..2478)
                     /locus_tag="VGB85_21615"
     CDS             complement(934..2478)
                     /locus_tag="VGB85_21615"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0136702.1"
                     /translation="MAQEEQDLEIGVETVTADDTLMTPGGEHPWHEPEALVGALLGER
                     YKLLRLIGQGGMGAVYLAEHVVIGKQIAVKVLNPEYSKNPGDVQRFLQEARAASLIRH
                     DHVVDIADFGNSPQGQAYLVMEYLEGEDLAQTLKSVGRMDWFRVSNIVMQIGSALAAA
                     HAKGIVHRDMKPENCFRVKRDGDADFIKVLDFGIAKIVDDRMSKGHQSLTIEGGIIGT
                     PEYIAPELCRGLKADSRVDIYALGVVMYRMLTGVLPFRTDTDNYMAVLSQHLTDPPVP
                     PREQAPDADIPPKIEQIVLRTLEKDPADRYQRIDELIAALREAQRTLTGSSSTAIMPG
                     LPPIPRRRSTKQPARRPVGLIVALIAVLLAGAAAITWTVLSGPGEPPPPVATLTPAEL
                     LPPAPPPPVPPAPVAAASTGPDTDTDEPDVTGDTTVPEALSTAEFRRVAGQIQRSLKG
                     RCPGLPGFEVGARVYVEASGKVARVVPEGRQAGSSFSACVAKLVRQTRFKKARKPSVH
                     VASFKL"
     gene            complement(2498..3403)
                     /locus_tag="VGB85_21620"
     CDS             complement(2498..3403)
                     /locus_tag="VGB85_21620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136703.1"
                     /translation="MSIAAPTSAAQLAPAGDDGLAAATATYNEGIAERDAGNFVAAAE
                     SFTEAYAAIPMQSREIRSAVLFDLVDARRQAFALGEGPAQICECERLLGSYLADVKQT
                     FGSKGDKFPDTRKAKKLLVEVRKQLTKQMLETPELDCAATTVARPPEPEPVPEPPPLP
                     PEPTGPSEAQLQAQAARDRRARTQVIAGAVTTGVGGLFLGVMAAGLVVGRRAEQAGTT
                     ATQEGLASGQPLSEDDPELRDIVARGKLGNGLAIAGGVLATVAVAAGVSLLVLGLRAR
                     KEPPRAAVAPALAPGFVGAGVVLRF"
     gene            4039..5028
                     /gene="meaB"
                     /locus_tag="VGB85_21625"
     CDS             4039..5028
                     /gene="meaB"
                     /locus_tag="VGB85_21625"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEY0136704.1"
                     /translation="MSKQRLDVDEYVAGVRAGDRALLGRAITLIESQRPDDQQRAQQV
                     VAALLPFTGGAHRVGITGVPGVGKSTLIEALGTHLTAQGRRVAVLAVDPSSLRSGGSI
                     LGDKTRMQSLARDPRAFVRPTPTLGALGGVARHTRETLLLCEAAGYDVVLVETVGVGQ
                     SEVMVAQLVDCFIVLMLAGAGDELQGIKRGILELVDILAITKADGDGAEAARRARSEY
                     AMALHLMRPRHPAWTVPVLTCSAYTQDGIAALWRAVVEHRDTLERAGAFTASRAQQQV
                     YWMWRAIEAGLLERFTRDPAVAAGIGGLEAEVRGGAVSPDHAAAQLLALFRRG"
     gene            complement(5029..>5441)
                     /locus_tag="VGB85_21630"
     CDS             complement(5029..>5441)
                     /locus_tag="VGB85_21630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136705.1"
                     /translation="WQGLVGIVYVVAPVPIMGMFAEGDVDAAELASLGAVLLAISAAW
                     QLFDAAVMTISEALRAAGDTAWVLYARVGLAWLVWLPLSMWVVYGLGGGPPAATACFL
                     VYFITLAVAVWWRFNSGAWRRIDLTGQSELPLVV"
BASE COUNT          764 a         1898 c         2009 g          770 t
ORIGIN      
        1 tgaccgggga gccgtacctg tgcggcgatg tacagagcga cgagcgcgag ctgatcggcg
       61 tgcgcgaggg gctcggggtg cagtcgtcga tcgcggtcgt gatcgtcgtc ggtgagcgcc
      121 gcggcgtgct gagcatcgcc tcgttgcagc cgcagcgctt cgacgaggcc gacctgcgct
      181 tcgtcgagac cgtcgcgcgc tggctcggga tcttgatcca tcgcaccgag ctggtcgagc
      241 gcgtcgcgca ggaggccgcc tcgcaggcgc gcaagatcgc cgcggaggag atcgtcacga
      301 tcgtcgcgca cgacctgcgc aaccacctcg cggcgatcca cgggcgcctc catctcatcg
      361 gtgcgcgcag ccgcaaggac ggccgcgcgc aggatcaggg cgacgccgat caggcgctca
      421 gggtgctcgc tcgcctgcgt agcatggtgt ccgacatgct cgacctgagc cgactcggcc
      481 gcggcagcct cgtcgtgtgc ccggagccgc tcgacctcgt catgctggtc caggagacgg
      541 tcgacctcct gcgcgcgccg aacctggaga tcacgatcac cggccccgag tcgctggtcg
      601 tcggcggcga tccgctgcgg ctgcgtcagg tgctcgagaa cctgctcggc aacgcgatca
      661 agcactcgcc gaccggggcg ccggtgttcg tgatgctcgc caagcaggcc cgaggggccg
      721 atgaattcgc ggtcgtcagc gtcaaggact ccggccccgg cgtcgacccg cggatcttgc
      781 cgcgcctgtt cgaccgcttc accaccggcc ccggctcgtc tggcctcggc ctcggcctgt
      841 acatcgcccg cggcatcacg gaggcccacg ggggcacgct gaccgtcgag tcggcgccgg
      901 ggcagggcgc gaccttctcg ctcgcgctgc cgctcacagc ttgaagctgg cgacgtgcac
      961 cgacggcttg cgcgcctttt tgaaacgggt ctggcgcacg agcttggcga cgcacgcgct
     1021 aaacgacgag cccgcctgtc ggccctcggg cacgacgcgg gcgaccttgc cgctcgcctc
     1081 gacgtagacg cgggcgccga cctcgaagcc gggcaggccc gggcagcgac ccttgagcga
     1141 tcgctggatc tggccggcga cgcggcgaaa ctcggcggtg gacagcgcct cgggcacggt
     1201 cgtgtcaccg gtgacgtccg gctcgtcggt gtcggtatcg gggccggtgc tcgcggcggc
     1261 caccggcgcc ggtgggaccg gtggaggtgg tgccggcggc agcagctcgg cgggcgtcag
     1321 ggtcgcgaca gggggcggcg gttcgccggg cccggagagc acggtccagg tgatcgcggc
     1381 ggccccggcg agcagcacgg cgatcagcgc gacgatcagg ccgacgggtc ttcgcgccgg
     1441 ctgcttcgtc gagcggcggc gcgggatcgg cgggaggccc ggcatgatcg cggtcgagct
     1501 cgagccggtg agcgtccgct gggcctcgcg cagcgcggcg atcagctcgt cgatccgctg
     1561 atagcggtcg gccgggtcct tctccagcgt gcgcagcacg atctgctcga tcttcggcgg
     1621 gatgtcggcg tcgggggcct gctcgcgcgg cggcaccggc gggtcggtca ggtgttggct
     1681 gagcaccgcc atgtaattgt cggtgtcggt gcgaaacggc agcacgccgg tcaacatgcg
     1741 gtacatgacg acgccgagcg cgtagatgtc gacgcgcgag tcggccttga ggccgcggca
     1801 cagctcgggg gcgatgtact ccggcgtgcc gatgatgccg ccctcgatcg tcagcgactg
     1861 gtggcccttg ctcatccggt cgtcgacgat cttggcgatg ccgaagtcga ggactttgat
     1921 gaaatcggcg tcgccgtcgc gcttcacgcg aaagcagttc tccggcttca tgtcgcggtg
     1981 gacgatgccc ttggcgtgcg cggccgccag cgccgagccg atctgcatga cgatgttgct
     2041 gacgcggaac cagtccatgc gcccgacgga cttcagggtc tgcgcgaggt cctcgccctc
     2101 gaggtactcc atcaccaggt aggcctggcc ctgcggcgag ttgccgaagt cggcgatgtc
     2161 gacgacgtgg tcgtggcgga tcagcgaggc ggcgcgggcc tcttgcagga agcgctgcac
     2221 gtcgcccggg ttcttgctgt actccgggtt gagcaccttg acggcgatct gcttgccgat
     2281 gacgacgtgc tcggcgaggt acaccgcgcc catgccaccc tggccgatca ggcgcaggag
     2341 cttgtagcgc tcgccgagca gcgcgccgac cagcgcctcg ggttcatgcc aggggtgctc
     2401 gccgccgggc gtcatcaggg tgtcgtcggc cgtgacggtc tcgaccccga tctccagatc
     2461 ttgctcctcc tgcgccacct tcgcgcagca tatcggatca gaagcgtagt acgacgcccg
     2521 cgccgacgaa gcccggggcc agcgccgggg ccacggcggc gcgcggaggc tccttgcgcg
     2581 cgcgcagacc gaggaccagc agcgagactc cggcggcgac cgcgaccgtt gccagtacac
     2641 cgcctgcgat cgcaaggccg ttgccgagct tgccgcgagc gacgatgtct cgcagctcgg
     2701 ggtcgtcctc ggacagcggc tgcccgctgg cgaggccctc ctgcgtcgcc gtggtgcccg
     2761 cctgctcggc cctgcgaccg accaccagcc cggccgccat cacgccgagg aacagcccgc
     2821 cgacgcccgt cgtcaccgcg ccggcgatca cctgcgtgcg ggcgcgccgg tccctggcgg
     2881 cctgggcctg cagctgcgcc tcgctcggcc cggtcggctc cggcgggagt ggtggcggct
     2941 cgggtacggg ctcgggttcg ggcggccttg cgaccgtcgt cgcggcgcag tcgagctcgg
     3001 gcgtctccag catctgtttg gtgagctgct tgcggacctc gacgagcagc ttcttggcct
     3061 tgcgggtgtc ggggaacttg tcgcccttgc tgccgaaggt ctgcttgacg tccgcgaggt
     3121 aggacccgag caggcgctcg cactcgcaga tctgcgccgg gccctcaccg agcgcgaagg
     3181 cctggcggcg cgcgtcgacg aggtcgaaca gcacggccga gcggatctcc cgcgactgca
     3241 tcgggatcgc cgcgtaggcc tccgtgaacg actccgcggc ggcgacgaag ttgccggcgt
     3301 cgcgctccgc gatgccctcg ttgtaggtgg cggtcgcggc cgcgaggccg tcgtcgcccg
     3361 ccggagccag ctgcgcggcg ctcgtcggtg cggcgatcga catggccaaa aggacagcca
     3421 gcacgcgcgc catcggccct cgcggggacg cgaggaaggg gcggtgcgat gcggcggcca
     3481 tcggaaggat gataggtcag ctcgctggca caggtacagc gacgaacgag ggcccgcgcg
     3541 atcgtgacac cggctcacag cgggcctggc gctgcgcatg gagaggtcac cgcacgactg
     3601 gcaggacgga cggcggtggt gtgatacgac gcggcgtcgg cgggtggaat gatgggcatg
     3661 cgcaagttgc agaggcgacg gctgcgagaa tccctgcaag aagacggtgc tgcagaagta
     3721 caccgcccac gcgccgcgcg tcagcgtcgt caccgacact ggcatgggca acgtcccgac
     3781 cgacgcgaac ggtagcggct ggtacttcaa cgcctcgtag ctcgtgcgac accggggcga
     3841 ccaactccga cctgcgcgtg tgcatccata ccaacgccaa cgcgacccag aaccgctgcg
     3901 gcaacaccta cctcaacgac aacggcgcgt gggagcgcgt cgtgctccac gcggactgag
     3961 gtcggcgcag gccggcgacc cgcccgggtc gccggcgcat gcgtgtggtg tgctcgcgcg
     4021 ggtatcgtcc cggcctcgat gagcaagcag cgcctcgacg tcgacgagta cgtggcgggc
     4081 gtacgcgccg gagaccgcgc gctgctcggc cgcgcgatca cgctgatcga gagccagcgc
     4141 ccggacgacc agcagcgggc ccagcaggtc gtcgcggcgc tgctgccgtt caccggcggg
     4201 gcgcatcgcg tcggcatcac cggggtgccg ggggtcggca agagcacgtt gatcgaggcg
     4261 ctcgggaccc acctgaccgc gcagggccgg cgcgtcgcgg tgctcgcggt cgacccgagc
     4321 tcgctgcgca gcggcggcag catcctcggc gacaagacgc gcatgcagtc gctggcccgc
     4381 gacccccggg ccttcgtgcg cccgacgccg acgctcggcg cgctcggcgg ggtcgccagg
     4441 cacacgcgcg agaccctgct gctgtgcgag gcggccggct acgacgtcgt gctcgtcgag
     4501 accgtcggcg tcgggcaaag cgaggtgatg gtcgcgcagc tggtcgactg cttcatcgtg
     4561 ctgatgctgg ccggcgcggg cgacgagctg cagggcatca agcgcggcat cctcgagctc
     4621 gtcgatattt tggcgatcac gaaggccgat ggtgatggcg ccgaggcggc caggcgcgcg
     4681 cgcagcgagt acgcgatggc cctgcacctg atgcggccgc gacacccggc gtggacggtg
     4741 ccggtgctga cctgctcggc gtacacgcag gacggcatcg ccgcgctgtg gcgcgcggtc
     4801 gtcgagcacc gcgacacgct cgagcgcgcc ggcgcgttca cggcctcgcg cgcgcagcag
     4861 caggtgtact ggatgtggcg cgcgatcgaa gccggactgc tcgagcgttt cacccgcgac
     4921 cccgcggtcg cggccggcat cggcgggctc gaggccgagg tccgcggcgg cgccgtcagc
     4981 cccgaccacg ccgccgcgca gctgctcgcg ctgtttcgcc gcggctgact acacgacgag
     5041 cggcagctcg ctctggccag tgaggtcgat gcgccgccac gcgccgctgt tgaaccgcca
     5101 ccacaccgcg accgccaggg tgatgaagta caccaggaag caggccgtgg cggccggtgg
     5161 cccgccgccg aggccgtaga cgacccacat cgacagcggc agccacacca gccaggcgag
     5221 gccgacgcgg gcgtacagca cccacgcggt gtccccggcg gcgcgcaggg cctcgctgat
     5281 cgtcatgacg gcggcgtcga acagctgcca ggcggccgag atcgccagca gcacggcgcc
     5341 gaggctggcg agctcggcgg cgtcgacgtc gccctcggcg aacatcccca tgatcgggac
     5401 cggcgcgacg acgtacacga tgccgaccag gccctgccag g
//
LOCUS       DASWNJ010001291         2169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_162779, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001291 DASWNJ010000000
VERSION     DASWNJ010001291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162779"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VGB85_21635"
     CDS             <1..804
                     /locus_tag="VGB85_21635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136706.1"
                     /translation="PTVFVPQTKIADDQAARVQALVGAGAARVASLGDPAGLRAAIAA
                     VLDPEVAAGLRAAGMAHVPHNHARDAALELLGLVLPAALLRQAREVVDDAALAEVGAL
                     GVELGVAVDVACALHIGRAAVDRATLELDAAIDLLRTAAAHTVPPATLLRLAGLWSRK
                     LRAPRVAPPEAVAEALTLLLEFPGLAGQWSAAPLLLGALTGEREWGPVELARQLCGLL
                     DVGLSAGRDVFATTRALVEAQQEQSEHGEGGSNAALLARARARLRGGGR"
     gene            801..1952
                     /locus_tag="VGB85_21640"
     CDS             801..1952
                     /locus_tag="VGB85_21640"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0136707.1"
                     /translation="MTAMTASAAKQRLQSLDDGVVRLGPQTVHLDITNSCNTDCVTCW
                     DHSPHLRAPPPTAWKRRRADPAALAALLDDITGLGGLEAVIVSGMGEPFTHPAVYEIL
                     ADIKRRGLHLTVITNLVAAEVERIVEIGVDSLLIGVQGASEASYLAFHPSFSALHWAR
                     LQAALTRLRAAGRRDKHVQVICGVNAHELVAMVEQADTFAAAQINFKLASLRAGTEAV
                     MISADQRERLLTRDVPAAIDRAAALGVAHNLDVFAQQLRTGGEATAPIAEVGCFLGYH
                     YSRITVDGAVLYCCNTEVEVGHLQDGFAALWRGPAWTALRARLRNGHYFAGCAQCGKF
                     NQNVKLSARFRQLHGEARWLAATGRGPGGAPSVAAPGRPATRKALPVLP"
     gene            1949..>2169
                     /locus_tag="VGB85_21645"
     CDS             1949..>2169
                     /locus_tag="VGB85_21645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136708.1"
                     /translation="MRRLPVLSEGAGPEVHVLEDWPAAPPWAHAPTVEWQICGHCNYD
                     CSYCIQSRKHRVGYPSDAEIERALAVLAGL"
BASE COUNT          266 a          830 c          804 g          269 t
ORIGIN      
        1 ccgacggtgt tcgtgccgca gaccaagatc gccgacgacc aggccgcgcg ggtgcaggcg
       61 ctggtcggcg cgggcgcggc ccgggtcgcc agcctcggcg accccgcggg cctgcgcgcc
      121 gcgatcgcgg cggtgctgga ccccgaggtc gcggccggcc tgcgcgcggc cggcatggcc
      181 cacgtgccgc acaaccacgc ccgtgacgcc gcgctcgagc tgctcggcct ggtgctgccc
      241 gccgcgctgc tgcgccaggc gcgtgaggtg gtcgacgacg cggcgctcgc ggaggtcggc
      301 gcgctcgggg tcgagctcgg cgtcgcggtc gacgtcgcct gcgcgctgca catcggccgc
      361 gccgcggtcg accgcgcgac gctcgagctc gacgcggcga tcgatctgct gcgcaccgcc
      421 gcggcccaca cggtcccgcc ggcgaccctg ctgcggctcg ccgggctgtg gagccgcaag
      481 ctgcgcgcgc cacgggtcgc cccgcccgag gcggtcgccg aggcgctgac gctgctgctc
      541 gagttccccg gcctcgccgg ccagtggtcg gccgcgccgc tgctgctcgg cgcgctgacc
      601 ggcgagcgcg agtggggccc ggtcgagctg gcgcggcagc tgtgcggcct gctcgacgtc
      661 ggcctgtcgg cgggccgcga cgtgttcgcg accacgcgcg cgctcgtcga ggcccagcag
      721 gagcagagcg agcacggcga gggcggcagc aacgcggccc tgctcgcgcg ggcgcgggcg
      781 cggctgcggg ggggcgggcg atgacggcga tgacggcgag cgccgcgaag cagcgattgc
      841 agagcctcga cgacggggtg gtccggctcg gcccgcagac ggtgcatctc gacatcacca
      901 acagctgcaa caccgactgc gtgacctgct gggaccacag cccgcacctg cgcgcgccgc
      961 cgccgaccgc gtggaagcgc cggcgggccg accccgccgc gctcgcggcg ttgctcgacg
     1021 acatcaccgg cctgggcggc ctcgaggccg tgatcgtctc gggcatgggc gagccgttca
     1081 cccaccccgc ggtctacgag atcctcgccg acatcaagcg ccgcgggctg cacctgaccg
     1141 tgatcaccaa cctcgtcgcc gccgaggtcg agcgcatcgt cgagatcggc gtcgacagcc
     1201 tgctgatcgg ggtgcagggc gccagcgagg cgtcgtacct ggcgtttcat ccgtcgttct
     1261 cggcgctgca ctgggcccgc ctgcaggccg cgctgacccg cctgcgcgcc gccggccgcc
     1321 gcgacaagca cgtgcaggtg atctgcggcg tcaacgcgca cgagctggtc gcgatggtcg
     1381 agcaggccga caccttcgcg gcggcgcaga tcaacttcaa gctcgcctcg ctgcgcgccg
     1441 gcaccgaggc ggtcatgatc tccgcggacc agcgcgagcg gctgttgacc cgcgacgtgc
     1501 cggcggcgat cgaccgcgcc gcggcgctcg gcgtggccca caacctcgac gtcttcgccc
     1561 agcagctgcg caccggcggt gaggcgaccg cgccgatcgc cgaggtcggc tgcttcctcg
     1621 gctatcacta cagccggatc accgtcgacg gcgcggtgct ctactgctgc aacaccgagg
     1681 tcgaggtcgg gcacctgcag gacggcttcg cggcgctgtg gcgcggcccg gcgtggaccg
     1741 ccctgcgcgc ccgcctgcgc aacggtcatt acttcgccgg ctgcgcccag tgcggcaagt
     1801 tcaaccagaa cgtcaagctc tcggcccgct tccgccagct ccacggcgag gcccgctggc
     1861 tcgcggcgac cggccgcggc cccggcggcg cgccgagcgt cgcggcgccc ggacgcccag
     1921 cgacccgcaa ggccctgccg gtgctgccat gaggcgactt cctgtgctgt ccgaaggggc
     1981 aggccccgaa gtacatgtcc tcgaggactg gcccgcggcg ccgccgtggg cgcacgcgcc
     2041 gaccgtcgag tggcagatct gcgggcactg caactacgac tgcagctact gcattcagag
     2101 ccgcaagcac cgggtcgggt acccgagcga cgccgagatc gagcgcgcgc tcgcggtgct
     2161 cgccggcct
//
LOCUS       DASWNJ010001292         4774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_306632, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001292 DASWNJ010000000
VERSION     DASWNJ010001292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306632"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VGB85_21650"
     CDS             complement(<1..232)
                     /locus_tag="VGB85_21650"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0136709.1"
                     /translation="MQVSRRNNVRIAGQGSPTLVFAHGFGCDQGMWRHVAPAFTDRHR
                     VITFDHVGAGGSDIRAYSRTRHASLAGYAADVL"
     gene            641..2143
                     /locus_tag="VGB85_21655"
     CDS             641..2143
                     /locus_tag="VGB85_21655"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0136710.1"
                     /translation="MPADFPSLRTHALACPRANVLQFETIDLHLAPGWTGLVGANGSG
                     KTTLLRLLAGDLAPAGGRVVRDPTDLSLVTCPQDVDECSPEITRLAEARGPARRLHGS
                     LHLEPRDLGRWSSLSPGERKRWQIGAALAAEPDVLLLDEPTNHLDAEGRDLLLHALRR
                     FTGVGLVVSHDRALLDQLTTRTLRIHRGALRCWAGSYTAARAAWEAERAAHNAERGAL
                     QARQRALAVQLGDARRELGAVDLQRSAGRRMKGLHDHDATGVLADGRVANAERKVGRR
                     VHLLRGELGRTAEALAGTHHDHERGAGVTLTGERAPTQHLMAIDLPELRVGDRRLLGP
                     VRLALTRDARVQLAGRNGAGKTTLLRALVASARCPPGRILEVPQDMSPETCQALLHEV
                     RRADAGERGRILTRLDALGVDPGALLATPRPSPGEARKLLLAQGLARGVWALVLDEPT
                     NHLDLPAIERLETALLDYPGAVLLVSHDAALASRVTNVTWTIADGEVHVH"
     gene            complement(2240..2785)
                     /locus_tag="VGB85_21660"
     CDS             complement(2240..2785)
                     /locus_tag="VGB85_21660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136711.1"
                     /translation="MKRWLALALVLACEAPAQPVVTAAPAVVEWRETRPVITAAVVPS
                     EPAAMAEWLQSFVYRQWPPQTEIRATGEHGGERLYFNAVLAASMRAGAAEHPVGSAAV
                     RELYAADLATLKGFAVMHKTGPSGPTGEGWYWYEVFSATAQAEPTVAGPAARGCVGCH
                     SHAVDFVHSPDTPRADPQPLL"
     gene            complement(2782..3420)
                     /locus_tag="VGB85_21665"
     CDS             complement(2782..3420)
                     /locus_tag="VGB85_21665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136712.1"
                     /translation="MSPRVPLFLVSLALACSVTNDPADTDASTSAASTGASGPTSTTG
                     STASTSSTTATASTATSATTGPDDPTTAGTTPATEASSGGSEDSSASEAGTAETGDGV
                     SFTAVYEQVIMPNACNSGYCHGGGAGGLELTDEATSYANLVDMAATSMVCGQSMRVVP
                     GSLEESILWYRVRPAALDAGNPCAPKMPQGSMGLSDAEAKLVDDWIAGGALE"
     gene            complement(3452..4624)
                     /locus_tag="VGB85_21670"
     CDS             complement(3452..4624)
                     /locus_tag="VGB85_21670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEY0136713.1"
                     /translation="MQKSSILLPALLGLTGCFKDTQGSADASASSTASEGSSTDTTSA
                     PTTGEDSDAGTDGATGGGDTTTGAPCPGVAQSLPPARPGVMFVLDRSGSMILPWDDDG
                     DVATPEVTRWSSLHAALATTLTTHEDRVAFGVATSPGQKATAQYSEAACVVANKAEVS
                     LAPDNASTILDKLPAADDDTLAGADPIAATLSNALAELESVHPALPRRIVLISNGLPQ
                     CHAGATAAELFEVYDADAPVVAAEALAAGVPVHVLGLGLTDQTTPIAQDGVPDSVGYF
                     AKYDELAMAGGTAPFVNATTGAALPAALAEVLEAALTGVDPCVVPLDVAATEPGQAIA
                     EIDGEPLARVEDCLVDSGWHFVGAAPHAAIELCGAACEALKTAGAVDVFYCEGAPD"
BASE COUNT          639 a         1805 c         1680 g          650 t
ORIGIN      
        1 cgagcacgtc cgcggcgtag ccggccagcg aggcgtggcg cgtgcggctg tacgcgcgga
       61 tgtccgagcc accggcgccg acgtggtcga aggtgatcac ccgatgacgg tcggtgaacg
      121 cgggggcgac gtgccgccac atgccctgat cgcagccgaa gccgtgggcg aagaccagcg
      181 tcggcgagcc ctggccggcg attcgaacat tattcctacg cgatacctgc acgaccccgc
      241 cccgaaaatc cgatcgatta atacgggccg tgcctggcct gtcaacctga ggcttacccg
      301 gatcgccgcg cacgcgcgcg tgagcgggcg ccgaggcggc gtcactctcg tgatttgcgc
      361 ggccaccgta attctccccg gctcgaaaac gccgcggtca cgtccatgca tgccgacctg
      421 acgcggtcac cgccagcgcg ggcaacttcg gcttgcacga cctcgacgct cgtggcaatt
      481 gcatcgctgc cgcgagctcc gcggcgtctc atgagcagca agcgctgaag ttcccgtccg
      541 tcgcgtccgg cccccacacc ttggtgtgtc tggtggagcc ggccgcatgt gtccgaccgc
      601 gccgtcgatc ctgcggcggc gccgtgaccc cggaggcttc atgcctgctg acttcccctc
      661 tttgcgtact catgcgctcg cgtgcccgcg cgcgaacgtc ctgcaattcg aaacgatcga
      721 ccttcacctc gcccctggct ggaccggcct cgtcggcgcc aacggctccg gcaagacgac
      781 cctgctgcgc ctgctcgcgg gggacctcgc ccccgcgggc ggtcgcgtcg tgcgcgaccc
      841 gaccgacctg tccctcgtga catgtccgca ggacgtcgac gaatgcagcc cggagatcac
      901 gcgactcgcg gaggcccgcg gccccgcgcg ccgcctccac ggcagcctgc acctcgagcc
      961 ccgcgacctc gggcgctggt cctcactgtc ccccggcgag cgcaagcgct ggcagatcgg
     1021 cgcggcgctg gcggccgagc ccgacgtcct gctgctcgac gagccgacca accacctcga
     1081 cgccgagggc cgcgacctgc tgttgcacgc gctgcgtcgc ttcaccggcg tcggcctcgt
     1141 cgtctcgcac gaccgcgcgc tgctcgacca gctgaccacc cgcacgctgc ggatccatcg
     1201 cggcgcgctg cgctgctggg cgggctccta cacggccgcc agggccgcgt gggaggccga
     1261 gcgcgcggct cacaacgccg agcgcggcgc cttgcaggcc cggcagcggg cgctcgccgt
     1321 gcagctgggc gacgcgcgcc gcgagctcgg cgcggtcgac ctccagcgca gcgcgggccg
     1381 gcggatgaag ggcctccacg atcacgacgc caccggcgtg ctcgccgacg gccgggtcgc
     1441 caacgccgag cgcaaggtcg gccggcgcgt gcacctgctg cgcggcgagc tcggtcgcac
     1501 cgccgaggcg ctcgcgggca cccaccacga ccacgagcgc ggcgccgggg tgaccctgac
     1561 gggcgagcgg gcgccgacgc agcacctgat ggcgatcgac ctgcccgagc tgcgggtcgg
     1621 cgaccggcgc ctgctcgggc cggtccgcct cgcgctgacc cgcgacgccc gcgtgcagct
     1681 ggccgggcgc aacggggccg gcaagacgac cctgctgcgc gccctcgtcg ccagcgcccg
     1741 ctgcccgccg gggcggatcc tggaggtccc gcaggacatg tccccggaga catgtcaggc
     1801 actgctgcac gaggtccgcc gcgccgacgc cggcgagcgc gggcggatct tgacgcggct
     1861 cgacgcgctc ggcgtcgacc ccggggcgct gctcgccacc ccgcggccct caccgggcga
     1921 ggcgcgcaag ctcctgctcg cgcagggtct ggcccgcggc gtgtgggcgc tggtgctcga
     1981 cgagccgacc aaccacctcg acctgccggc gatcgagcgc ctcgagaccg cgctgctcga
     2041 ctatcccggc gcggtcctgc tggtcagcca cgacgccgcg ctggcgagcc gcgtcacgaa
     2101 cgtcacctgg acgatcgccg acggcgaggt gcacgtgcat tgaaaaacct tcagggcgtg
     2161 atgatcccga tgatcccgat gatcccgatg atccatcgtt ttacgcctgc acggatcgat
     2221 gcacccacgc aagcgccgat cacagcagcg gctgcgggtc cgcgcgcggg gtgtccggcg
     2281 agtgcacgaa gtcgaccgcg tgcgagtggc agccgacgca cccgcgggcg gccggtccgg
     2341 cgactgtcgg ctcggcctgc gcggtggcac tgaacacctc gtaccagtac cagccctcgc
     2401 cggtcggccc cgaggggccc gtcttgtgca tcaccgcgaa gcccttgagc gtcgcgagat
     2461 cggcggcgta cagctcgcgc accgcggcgc tgccgaccgg gtgctcggcc gcgccggccc
     2521 gcatcgacgc cgcgagcacc gcattgaaat acaggcgctc gccgccgtgc tcgcccgtcg
     2581 cgcggatctc cgtctgcggc ggccattgcc ggtacacgaa cgactgcaac cattcggcca
     2641 tcgccgcggg ctccgacgga acgaccgcgg cggtgatgac cggtcgcgtc tcacgccact
     2701 cgaccacggc gggcgcggcg gtgacgaccg gctgcgcggg cgcctcacac gcgagcacga
     2761 gcgcgagcgc gagccacctt ttcactcgag cgcgccccct gcgatccagt cgtcgacgag
     2821 cttcgcctcg gcgtccgaca ggcccatcga gccctgcggc atcttcggcg cgcacgggtt
     2881 gccggcgtcg agcgccgcgg ggcgcacgcg ataccacaag atgctctcct cgagcgaccc
     2941 ggggaccacg cgcatgctct ggccacatac catcgaggtc gccgccatat cgacgaggtt
     3001 cgcgtagctg gtcgcctcgt cggtcagctc gagcccgccg gccccgccgc cgtggcaata
     3061 cccggagttg caggcgttcg gcatgatgac ctgctcataa acggcggtga aggacacgcc
     3121 atcgccggtc tccgccgtgc cggcctcgct cgccgagctg tcctccgatc caccactgct
     3181 cgcctccgtc gccggggtcg tgcccgcggt ggtcgggtcg tccggcccgg tcgtggcgga
     3241 tgtcgcggtc gacgcagtag cggtggtcga cgaggtcgac gcggtcgaac cggtcgtgct
     3301 cgtcgggccg ctcgcgccgg tgctcgcggc cgaggtcgat gcatccgtgt cggcggggtc
     3361 gttggtcacg gagcacgcga gcgcgaggct cacgaggaac aggggtacac ggggcgacat
     3421 cggccgacga tacggacacg cgcgctcgcg gtcaatcggg ggcgccctcg caatagaaca
     3481 catcgaccgc gccggcggtc ttgagcgcct cgcaggcggc cccgcacagc tcgatcgcgg
     3541 cgtggggcgc cgcgccgacg aagtgccatc cgctgtcgac gaggcagtcc tcgacgcgcg
     3601 cgagcggctc gccgtcgatc tcggcgatcg cctgcccggg ctcggtcgcg gcgacgtcga
     3661 gcgggacgac gcagggatcg acgccggtga gcgcagcctc gagcacctcg gcgagcgcgg
     3721 ccggcagcgc ggccccggtg gtcgcgttga cgaacggcgc cgtcccgccg gccatcgcca
     3781 gctcgtcgta ttttgcgaag taaccgacgc tgtcgggcac gccgtcctgg gcgatcggcg
     3841 tggtctggtc ggtcaggccg agcccgagga cgtgcaccgg gaccccggcc gcgagggcct
     3901 cggccgcgac gaccggcgcg tcggcgtcgt agacctcgaa cagctccgcc gcggtggcgc
     3961 ccgcgtgaca ctgcgggagg ccgttggaga tcagcacgat ccgccgcggc agcgccgggt
     4021 ggaccgactc gagctcggcg agcgcgttgc tcagagtcgc cgcgatcggg tcggcgcccg
     4081 cgagcgtgtc gtcgtccgcc gccggcaact tgtcgaggat cgtgctggca ttgtccgggg
     4141 ccagcgagac ctcggccttg ttcgcgacga cacacgccgc ctcggagtac tgcgccgtcg
     4201 ccttctgacc cggcgaggtc gccacgccga acgcgacgcg gtcctcgtgg gtcgtcagcg
     4261 tcgtcgctag cgccgcgtgc aggctgctcc atcgcgtcac ctccggcgtc gcgacgtcgc
     4321 cgtcgtcatc ccacggcagg atcatcgagc ccgagcggtc gagcacgaac atcacgcctg
     4381 ggcgcgccgg cggcaacgac tgggcgacgc ccggacacgg cgcccccgtc gtcgtgtcgc
     4441 cgccgcccgt ggccccgtcc gtgcccgcgt ccgagtcctc gcccgtcgtc ggcgcggacg
     4501 tcgtatccgt gctcgacccc tccgaggcag tggacgaggc gctcgcgtcc gcggacccct
     4561 gggtgtcctt gaagcatccg gtgaggccga gcagcgccgg caggaggatc gacgactttt
     4621 gcatgggcat gagactcagg cgccgggcgg tccggcggtg catcacgcta acacgtctcg
     4681 tcgcgacgac ggtcgactcc gcgcggtcgc accggcggct tcgagacccc acgggcgcta
     4741 acatccggcg ccgcgatatg cacggcgcgg gcct
//
LOCUS       DASWNJ010001293         3893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_515888, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001293 DASWNJ010000000
VERSION     DASWNJ010001293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515888"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3893)
                     /locus_tag="VGB85_21675"
     CDS             complement(<1..>3893)
                     /locus_tag="VGB85_21675"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEY0136714.1"
                     /translation="AAVEIWLRALQGAPPRLELPARAAAGGEGASAERTLDAALCERL
                     GEIAGGKPLAAVLLASLWVLVARHTGQDDIVALYGGAGAQAPARGRLEDDPSFAELVV
                     RADAAIVEAERWPVELSALDEGLARGGQALAWQVSLRLVDGVAPVDAGVHSLAVELRP
                     DANGLWIGLRGDPSLFDGETLRAWAMRWEVLLRGAAQGPERPVSALPLLSAQERAAAA
                     AAWNDTAMALPTEQCVHSLVDAQIARTPDAIAVIAEEGQLSYAELGARASRLAAALRR
                     RGVGPEVLVAICAERSLDLIVGLLAILRAGGAYVPLDPDHPRDRLAYMLADTSARLLL
                     TQRALVAGLPAFDGEILLLDELDELDELADIADPAAGEGPAVTASNLVYVIYTSGSTG
                     RPKGAMNIHRGVVNRLLYLQRVDPLGPDDRVLQKTPCSFDISVYEYFWPLLAGATLVM
                     ARPGGHKDPGYLLEVIREHSITIIHFVPPMLAVFLEQADLSACGSLRRVLCTGDVLPP
                     AVVQRCFMRLDVELYNLYGPTEASIEVTMYRCRRDEACAVVPIGQPMANTTIYLLDRH
                     GEPVPPGAPGELFIGGVQVGRGYLGRPDLTAASFVPDPFSDDPGARLYRTGDLARRRA
                     DGELEFLGRRDQQAKIRGVRVELGEIEAMLREHPGVRDVVVVVSQAAGGDKQLAAYLL
                     ANEGAVIEAAGLHAWACERLPVYMVPGAFMVLESFPLLSNGKLDRKSLPPAVAFRPVS
                     SAEPRTETERAVAALWYEVLGLERCGVDDDLLMLGGHSLHAAQLLGRVRDGFAVELVF
                     PEIFAHPTIAALARLIDERRGAATAETRAPAPAEALSPAEERLFFLHGLAPDSPAYHC
                     PSIFRVRGPFAPAQLESAVRAVVARHEVLRARFIEVDGEARRITSPDDSFELRTVELP
                     GASADDLLGYLAAEVARPFDLRSAPPIRAGVLRIAADDHVLWLNLHHIVTDGRSTAIL
                     LREVSAVYEALCAGEAPALPGPPLAHAEVVALQRAALASEAPRLERWKQRLAGVSRLE
                     LPVDGPCPGAPTWRGGQVGFAVSGELFDRLRSLARRRGATPSMAILAVIAAWLRRVTG
                     QDDRVIALIAAARGRSALENVVGFLVDTLPIRVELAGEAGFGALLEHVRARALEAQDG
                     EHVPLERVVKALALPRDAGRDPLIQVAFAPQPAGERDLRLAGAMVEPITVDRGAAIFD
                     LTILTWEAAGGGLEVVFEYASDLFSRARAESMMRSFAAVFAAAVDEPEAPVARLAMAS
                     ASEREALLAASHGPQPARERVGAVHAYFEAQAERTP"
BASE COUNT          595 a         1571 c         1275 g          452 t
ORIGIN      
        1 ggcgtccgct cggcctgcgc ctcgaaatag gcgtgcacag cacccacgcg ctcgcgcgcc
       61 ggctgcggcc cgtgcgacgc cgcgagcagc gcctcgcgct cgctcgccga ggccatcgcc
      121 aggcgggcca ccggggcctc cggctcgtcc acggcggccg cgaacacggc cgcgaagctg
      181 cgcatcatcg actcggcgcg ggcccgactg aacagatcgc tcgcatactc gaacacgacc
      241 tcgaggccgc cgcccgcggc ctcccaggtc agtatcgtca gatcgaagat cgccgccccg
      301 cggtcgaccg tgatcggctc gaccatcgcg ccagccagcc gaaggtcgcg ctcacccgcc
      361 ggctgcggcg caaacgcgac ctggatcagc ggatctcggc ccgcgtcacg cggcagcgcg
      421 agcgccttga ccacgcgctc gagcggcacg tgctcgccgt cctgcgcctc gagggcgcgc
      481 gcgcgcacgt gctcgagcag cgccccgaag cccgcctccc ccgcgagctc gacccggatc
      541 ggcaaggtat cgacgaggaa gccgaccacg ttctcgagcg ccgaacgccc gcgcgccgcg
      601 gcgatcagcg cgatcacccg atcgtcctgg ccggtgaccc gccgcagcca cgcggcgatc
      661 accgcgagga tcgccatcga cggcgtcgcg ccccggcggc gcgcgagcga acgcagccgg
      721 tcgaacagct cgcccgacac cgcgaagccg acctgtcccc cgcgccaggt cggcgcgccc
      781 gggcatgggc cgtcgacggg cagctcgagc cggctcacgc ccgcgagccg ctgcttccag
      841 cgctcgagcc tcggcgcctc gctcgccaga gccgcccgct gcagcgcaac gacctccgcg
      901 tgggccagag gcggaccggg cagcgccggc gcctcaccgg cgcacagggc ctcgtacacc
      961 gccgagacct cgcgcagcag gatcgcggtc gagcgcccgt ccgtgacgat atggtgcagg
     1021 ttcaaccaca gtacgtgatc gtccgcggcg atccgcaaca cgcccgcgcg gatcggcggc
     1081 gcagaccgca gatcgaaggg ccgcgcgacc tcggcggcga ggtaccccag cagatcgtcc
     1141 gccgacgcac cgggcagttc gacggtcctg agctcgaacg agtcgtccgg cgacgtgatc
     1201 cgccgcgcct cgccgtcgac ctcgatgaag cgcgcgcgca ggacctcgtg cctcgccacc
     1261 accgcgcgca ccgccgattc gagctgcgcc ggcgcgaacg gcccgcgcac gcgaaagatg
     1321 gacgggcagt gatacgccgg gctgtccggg gccagaccgt gcaggaagaa caggcgctcc
     1381 tctgcgggcg acagcgcctc tgcgggcgcc ggcgccctcg tctccgccgt cgccgcgccg
     1441 cgccgctcgt cgatcagccg cgccagcgcg gcgatcgtcg gatgagcgaa gatctccggg
     1501 aacaccagct cgaccgcgaa gccgtcgcgc acccgaccga gcagctgcgc cgcgtgcaat
     1561 gaatggccgc cgagcatcaa caggtcgtcg tcgaccccgc agcgctcgag ccccagcacc
     1621 tcgtaccaca gagccgccac cgcgcgctcc gtctcggtcc gtggctcggc gctgctcacc
     1681 ggccgaaacg cgaccgccgg cggcaacgac ttgcggtcga gcttgccgtt cgacagcagc
     1741 gggaagctct ccagcaccat gaacgcgccc ggcaccatgt acaccggcag ccgctcgcag
     1801 gcccacgcgt gcagccccgc cgcctcgatc accgcgccct cgttcgccag caggtacgcc
     1861 gccagctgct tgtcaccgcc ggccgcctgg ctcaccacca ccacgacgtc gcggaccccc
     1921 gggtgctcgc gcagcatcgc ctcgatctcg ccgagctcga cccgcacccc gcggatcttg
     1981 gcctgctgat ctcttcgacc gaggaactcg agctccccgt ccgcgcgccg gcgggccagg
     2041 tcgcccgtgc gatatagtcg agcgcccggg tcgtccgaga aggggtcggg cacgaagctc
     2101 gccgcggtca gatcgggcct cccgaggtac ccgcggccga cttgcacccc gccgatgaac
     2161 agctccccgg gcgcaccggg cggcactggc tcgccgtgtc ggtcgagcag gtagatcgtc
     2221 gtattcgcca tcggctgacc gatcggcacc accgcgcacg cctcgtcgcg gcgacaacgg
     2281 tacatggtga cctcgatcga cgcctcggtc ggcccgtaga ggttgtagag ctcgacgtcc
     2341 agccgcatga agcagcgctg gaccactgcc ggcggcagca catcgcccgt gcacagcacc
     2401 cgccgcagcg atccgcaggc cgacaggtcg gcctgctcga ggaacaccgc gagcatcggc
     2461 gggacgaaat gaatgatcgt gatcgaatgc tcgcggatca cctcgaggag gtaccccgga
     2521 tccttgtgcc cgcccgggcg cgccatcacc agggtcgccc cggcgagcag gggccagaaa
     2581 tactcgtaca ccgagatgtc gaagctgcac ggcgtctttt gcagcacccg gtcgtccggc
     2641 cccagcggat cgaccctctg taagtacagc agccgattga ccaccccgcg gtggatgttc
     2701 atcgcgcctt tcggtcgacc ggtcgagccc gaggtataga tcacatacac gaggttcgag
     2761 gcggtcaccg ccggcccctc gcctgccgcg ggatccgcga tatccgcgag ctcgtcgagc
     2821 tcgtcgagct cgtcgagcag cagtatctcg ccgtcgaacg ccggcaggcc ggcgaccagc
     2881 gcccgctggg tcagcagcag cctcgcgctg gtgtcggcga gcatgtaggc cagccgatcg
     2941 cgcgggtggt ccggatcgag cggcacatac gcgcccccgg cccgaaggat cgccagcagg
     3001 ccgacgataa ggtccagcga gcgctcggcg cagatcgcca ccagcacctc ggggccgacc
     3061 ccgcgccgcc gcagcgccgc cgccagtcgc gacgcacggg ccccgagctc cgcgtagctg
     3121 agctgtccct cctccgcgat caccgcgatc gcgtccggcg tgcgagcgat ctgagcgtcg
     3181 acaagagagt gcacacattg ttcggtcggc agcgccatcg ccgtgtcgtt ccacgccgca
     3241 gccgcagccg cgcgctcctg cgcggagagc agcggcaagg ccgacaccgg ccgctccggc
     3301 ccctgcgccg cgccgcgcag gagcacctcc cagcgcatcg cccaggcccg cagcgtctca
     3361 ccgtcgaaca gcgaggggtc tccccgcagg ccgatccaca ggccgttcgc gtcgggcctc
     3421 agctccaccg ccagcgaatg gacaccggcg tcgaccggcg cgaccccgtc caccagccgc
     3481 agcgagacct gccacgccag cgcctgaccg ccgcgcgcga ggccctcgtc gagcgcgctg
     3541 agctcgaccg gccaccgctc cgcctccacg atcgccgcgt cggcgcggac caccagctcg
     3601 gcgaacgagg gatcgtcctc cagccgcccg cgcgcgggcg cctgcgcacc cgcgccgccg
     3661 tacagcgcca cgatgtcgtc ctgccccgtg tgccgcgcga ccagcaccca gaggctcgcc
     3721 agcagcaccg cggccagcgg cttgccaccg gcgatctcac cgagccgctc gcacagcgcc
     3781 gcgtcgagcg tgcgctccgc cgacgcaccc tcgccgcccg cggccgctcg cgcgggcagc
     3841 tccagccgcg gcggcgcgcc ctgcagcgcg cgcaaccaaa tctccaccgc cgc
//
LOCUS       DASWNJ010001294         5087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_234800, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001294 DASWNJ010000000
VERSION     DASWNJ010001294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234800"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="VGB85_21680"
     CDS             <1..821
                     /locus_tag="VGB85_21680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136715.1"
                     /translation="AVGIEALGTSGQVVRALAVDQEGACFAVGLAGVMGDWDFAYWRI
                     DTTGEQTLDNTYDYRPSGAPPHSFGDLASDVVIDGDVAWIVGMSKGKHDAEQKDQSLY
                     LRGVLVPMNLHTGKLAGPVIVAPPVAGVWPQSAFFGATLHPAGVLVTGYGCDKTCSTY
                     RIETSRYSVDGVRTWFEFEADNDGLAYGSDVALDGQGRALVSAAVTQNGKLRGYVFGR
                     KIGPPGAVLFHHWYPGAGLSEALGIARDSFDRIFPVGYITVNGETEARVTRIHG"
     gene            complement(850..1953)
                     /locus_tag="VGB85_21685"
     CDS             complement(850..1953)
                     /locus_tag="VGB85_21685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136716.1"
                     /translation="MRVLILAALVLATACGDAAAPGFSTGPAITSVSGSTSGSASSGD
                     TSSTGPADDSAGSSTSTSSSTGAATLDVGTVMDFGPGQPPGCKGKVDLLFVISRQHTM
                     LTEQTQLLASFGGFIDTIEQKLEGFDVHIMTANPDGDWPGDVCQYQPEGCKTNWPACG
                     DWAEGYNCETYPEMVTSCDRKLGAGLIFNTGGGAANKLCDLQDGKRYIMSGEPDMAGA
                     LECIAKVGWSGGAAAMGDAMIAALSPALNGAGGCNEGFLRKEALLVVALITDWADEDS
                     KSYAKQQYEAIIAAKGDPRAVVMLAVVKNAPGEFEPAEACDYEEPSYGIFEDLLSRFP
                     YTVFGDTCTPSFAPFFDQAADKIGEACGSFIPQ"
     gene            complement(2063..3832)
                     /locus_tag="VGB85_21690"
     CDS             complement(2063..3832)
                     /locus_tag="VGB85_21690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0136717.1"
                     /translation="MLRTTCLALVLPLLACGDSGQDSASESSATTQATLPPPGMTGTT
                     TTTPTSTGTDATDGTASASMGETVDPDTGPVPTTDVVTSAGSATELPPDTSTSSGTTT
                     GVDTGTTADPSVGDTSTTEPIDVCKVHEDMEISDCAVVAPPNSFEPEIQWTWTGVQEP
                     YSIVTPLVANLTDDNGDGAIDLCDVPDVVVVASISSGFPDTVGHIYVLDGATGTLHFQ
                     IATAVDHTVTPAIGDIDADGLPEIVTSIVGGNPVAFEHDGAMKWQSATGWPEAYSGAL
                     ALADADNDGDVEILAGNRLYDHLGAHLFTAPQSAGNWSASAFADLDGDGDQEIVLGHA
                     AYHHDGAPLYVTGVFPGYPSIANLDADPEPEVLVTNQNGLALIDHDGTVQYQNMRPTG
                     DPPSATTWLRPSTVHDFDGDGPAEFATSSANNYTVYKPTGPTIVWKTPVSDQSGIAAG
                     TAFDFLGDGVAEAMYADEHFMHIFGATGEVLLKIPRTSGTLSEYPIVADIDNDGSAEI
                     VVVSCQLHPNPVSSPTVQVIRDKEDRWIQARRIWNQHTYHVTNVREDGTIPQFEKPSW
                     KALNTYRTNSQIEGGAVCDPKPQ"
     gene            complement(4037..>5087)
                     /locus_tag="VGB85_21695"
     CDS             complement(4037..>5087)
                     /locus_tag="VGB85_21695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136718.1"
                     /translation="GGSLVGFAPISDTDIWAFDEVTVVRWNGTRWDDLSPGPVSALAS
                     DYDVRHQMNDLHVDREGRVWASALRTLHLRDGDVWRNLVPALNLKGEQINAIVADTAG
                     VVHLHLSDHSFLRVVPGDRDRVTSVPPLPGTTWHDEEQVTIVGVDGKRRTYAGGPDFP
                     GRNYGYFIGDASGRLWTLTSAGLAVLGPGDARTVWPIGTLPALSGASRDILVAGRGPQ
                     TLPPVGPPRTGGLRGVVYLDGLPLPRAAIELCPHPLMIQPRPCEGSEPTLAGSTTPDG
                     SWEIKDVPLGSLGITVQTPEGWKLAFQAWKLLDKQRLKEGETLDLGSIDVSGGMVAQE
                     MPWQVKPKRGARATK"
BASE COUNT          719 a         1787 c         1741 g          840 t
ORIGIN      
        1 aggcggtggg gatcgaagct ctgggcacct cgggccaggt ggtccgagcg ctcgcggtgg
       61 accaggaagg ggcctgcttc gcggtggggc tcgccggggt catgggcgac tgggacttcg
      121 cgtactggcg catcgacacc acgggcgagc agacgctcga caacacgtac gactaccgcc
      181 cgtccggcgc accaccgcat agcttcgggg acctcgccag cgacgtcgtg atcgacggtg
      241 acgtcgcctg gatcgtcggc atgagcaagg ggaagcacga cgccgaacag aaggaccaga
      301 gcctctacct gcgcggggtc ctcgtgccga tgaacctcca caccggcaag ctcgccggtc
      361 ccgtgatcgt cgccccgcca gtcgctggcg tctggcctca aagcgcattc ttcggcgcga
      421 cgctccatcc cgcgggcgtg ctggtcactg gttacgggtg tgacaagacc tgctcgacgt
      481 accggatcga gacctcgcgc tacagcgtcg atggcgtgcg cacgtggttc gagtttgagg
      541 ccgacaacga cggcctcgcc tacggcagcg acgtcgcgct cgacggtcag gggagggcgc
      601 tcgtctcggc cgcggtgacg cagaacggca agctgcgcgg ctacgtgttc ggccgcaaga
      661 tcggcccgcc aggcgcggtc ctgttccacc actggtaccc gggcgccggc ctctcggagg
      721 ccctcggcat cgcccgcgac agcttcgatc gcatcttccc ggtcggctac atcaccgtca
      781 acggcgagac cgaggcccgc gtcacgcgca tccacgggta agctcgcggc gcgaggccgt
      841 gtagcgaggt cactgcggta tgaagcttcc gcacgcctcg ccgatcttgt ccgcggcctg
      901 gtcgaagaac ggcgcgaagc tcggcgtaca ggtgtcgccg aacacggtgt aggggaaccg
      961 cgatagcagg tcctcgaaga tgccgtacga gggctcctcg tagtcgcagg cttccgcggg
     1021 ctcaaactcc cccggcgcgt tcttcacgac cgcgagcatg acgacggcgc gcgggtcccc
     1081 tttggctgcg atgatcgctt cgtactgctg cttcgcgtac gactttgagt cctcgtcagc
     1141 ccagtccgtg atcaacgcga cgacgagcag cgcttccttg cgcaagaacc cctcgttgca
     1201 gccgcccgcg ccgttgagcg ccggggacag cgcggcgatc atcgcgtcgc ccatcgccgc
     1261 ggcgccgccg gaccatccga ccttggcgat gcactcgagt gcgcccgcca tgtccggctc
     1321 gccgctcatg atgtagcgct tgccgtcttg caggtcgcac agcttgttcg ccgcgccccc
     1381 gccagtgttg aagatcaacc cggcgccgag cttcctgtca cacgacgtca ccatctcggg
     1441 gtacgtctcg cagttgtaac cctcggccca gtcgccgcac gctggccagt tcgtcttgca
     1501 gccctctggc tggtactggc agacgtcgcc cggccaatcg ccgtccgggt tggcggtcat
     1561 gatgtggacg tcgaagccct cgagcttctg ctcgatcgtg tcgatgaagc ccccgaagct
     1621 ggccaggagc tgcgtctgct cggtcaacat cgtgtgctgg cgcgagatca cgaacagcag
     1681 gtccaccttg cccttgcagc cgggcggctg gccgggcccg aagtccatca cggttcccac
     1741 gtccagcgtg gccgcgcctg tactcgagct cgtgctcgtg gagctgccag ccgagtcgtc
     1801 ggcggggccg gtagacgatg tgtcgccaga ggacgcgctg ccactcgtcg aacccgacac
     1861 gctcgtgatc gccggcccgg tcgagaaccc gggcgcggca gcgtccccgc aggcggtggc
     1921 gaggaccagg gcggcgagga tgaggacgcg catgacctgg gtagcgtaga cctccccgca
     1981 cctgctgtca ccaggcatcc ccagcagtcc cgccggagcc gatctgttgc gccgccagtg
     2041 gtgtcagcgg ggcgacaggt cctcactgcg gcttggggtc gcagacggcc ccgccctcga
     2101 tctgcgagtt ggtacgatac gtgttgagcg ccttccacga gggcttctcg aactgcggga
     2161 tcgtgccgtc ctcgcgcacg ttggtcacat gatacgtgtg ctggttccag atccggcgcg
     2221 cctggatcca ccggtcctcc ttgtcgcgga tcacctgcac ggtcggcgac gacaccgggt
     2281 tcggatgcaa ctggcacgat accaccacga tctccgccga gccgtcgttg tcgatgtcgg
     2341 cgacgatcgg gtactcgctc agcgtccccg acgtccgcgg gatctttagc agcacctcgc
     2401 cggtcgcgcc gaagatgtgc atgaagtgct cgtcggcgta catcgcctcg gcgacgccgt
     2461 cgccgaggaa gtcgaacgcg gtgcccgccg cgatcccgct ctggtcggac accggcgtct
     2521 tccagacgat cgtcgggcct gtcggcttgt acaccgtata attgttggcc gagctggtcg
     2581 cgaattcggc cggcccgtcg ccgtcgaagt cgtggacggt cgagggccgc agccaggtcg
     2641 tcgcgctcgg cgggtcgccg gtcgggcgca tgttctgata ctgcacggtc ccgtcgtgat
     2701 cgatcagcgc cagcccgttt tggttggtga ccagcacctc cggctccggg tcggcgtcga
     2761 ggttggcgat cgacgggtag ccggggaaga cgccggtgac gtagagcggc gcgccgtcgt
     2821 ggtggtacgc cgcgtggccg agcacgatct cctggtcgcc gtcaccgtcg agatcggcga
     2881 acgccgaggc cgaccagttg ccggccgact gcggcgccgt gaacaggtgg gcgccgaggt
     2941 ggtcatacag ccggttgccg gcgaggatct cgacgtcgcc gtcgttgtcg gcgtcggcca
     3001 gcgcgagcgc ccccgagtac gcctccggcc agccggtcgc gctctgccac ttcatggcgc
     3061 cgtcgtgctc gaacgcgacc gggttgccgc cgacgatcga cgtcacgatc tcgggcaggc
     3121 cgtccgcgtc gatgtcgccg atcgccggcg tcaccgtgtg gtcgaccgcc gtggcgatct
     3181 ggaagtgcag cgtcccggtc gccccgtcga gcacgtagat gtgccccacc gtgtccggga
     3241 acccggagct gatcgacgcg acgacgacga catcggggac gtcgcacagg tcgatcgccc
     3301 cgtcgccgtt gtcgtcggtc aggttggcga ccagcggcgt gacgatgctg tacggctcct
     3361 gcacgcccgt ccacgtccac tggatctccg gctcgaagct gttcggcggg gccaccacgg
     3421 cgcagtcgct gatctccatg tcctcgtgga ccttgcacac gtcgatcggc tcggtcgtcg
     3481 acgtgtcccc gaccgatggg tcggccgtcg tcccggtgtc gaccccggtg gtcgtgccgc
     3541 tcgaggtcga cgtgtccggg ggcagctccg tcgccgatcc cgccgacgtc accacgtccg
     3601 tcgtcggtac ggggccggtg tccggatcca ccgtctcccc catcgatgcg ctcgccgtcc
     3661 cgtcggtcgc gtccgtgccg gtcgacgtcg gcgtcgtcgt cgtcgtcccc gtcatcccgg
     3721 gagggggcaa cgtcgcctgg gtcgtcgccg acgactcgga cgcggagtcc tggccgctgt
     3781 cgccgcaggc caggaggggg aggacgagcg cgagacaggt cgttcgaagc atcgggtcag
     3841 cgtcgccagc cggcgcgatg cggtcaaggg acggagccgc gacgcgcgac cccgtccggt
     3901 gcgatcgcac gcgtgccacg ctcgtccgcg acccactgcc cacaatttca agccgtcgag
     3961 tccacgaggc ccgcgtgcga atcgctcgac aacttcggtc cggcatgccc gcgcgttcgc
     4021 gggctgctcg accgcctcac ttcgtcgccc gcgcgcctcg cttcggcttg acctgccacg
     4081 gcatctcctg cgcgaccatg ccgccgctca cgtcgatcga tccgaggtcg agggtctcgc
     4141 cttccttcag gcgctgcttg tcgagcagct tccaggcctg gaacgcgagc ttccagccct
     4201 cgggggtctg caccgtgatg ccgagcgatc cgagcgggac gtccttgatc tcccaggagc
     4261 cgtcgggtgt ggtgctgcct gcgagggtcg gctcgctgcc ctcgcatggg cgcggctgga
     4321 tcatcagcgg gtgcgggcac agctcgatcg cggcgcgcgg cagcggcagg ccatcgaggt
     4381 acacgacccc gcgcaggccg cccgtgcgcg gcgggccgac gggaggcagg gtctgcgggc
     4441 cgcggccggc caccaggata tcgcgcgatg cacccgacag cgccggaagc gtgccgatcg
     4501 gccacacggt gcgcgcgtct cccgggccga gcaccgccag cccggcgctc gtcaatgtcc
     4561 acagtcggcc gctcgcgtca ccgatgaaat acccatagtt gcgccccggg aagtcgggcc
     4621 cgccggcgta tgtcctgcgc ttgccgtcga cgccgacgat cgtgacctgc tcctcgtcgt
     4681 gccacgtggt gcccggcaac ggcggcacgc tcgtcacgcg gtcgcggtcg cccggcacga
     4741 cccgcaaaaa gctgtgatcc gacagatgca ggtgcacgac gccggccgtg tccgcgacga
     4801 tcgcgttgat ctgctcgccc ttgaggttga gcgcggggac gaggttgcgc cacacgtcgc
     4861 cgtcgcgcag gtgcagcgta cgcagcgcgc tggcccacac gcggccctcg cggtcgacgt
     4921 gcaggtcgtt catctgatgg cggacgtcat agtcgctggc gagcgcggag actggccccg
     4981 gggacaggtc gtcccagcgc gtgccgttcc agcggaccac cgtgacctcg tcgaaggccc
     5041 agatgtccgt gtccgagatc ggcgcaaagc cgacgagaga gcctccg
//
LOCUS       DASWNJ010001295         1532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_335360, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001295 DASWNJ010000000
VERSION     DASWNJ010001295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335360"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1532
                     /locus_tag="VGB85_21700"
     CDS             <1..>1532
                     /locus_tag="VGB85_21700"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEY0136719.1"
                     /translation="AVEDKGRWSYKYETVDVPAGSCQLTSDVAPATCELDVGKAGTYV
                     ARAEIKDAKGRRALTRHTLYVYGKDAVVWDQDQRRVDLVPDKRSYEPGEQATVLLRSP
                     FDRARGLAVIEREGIVEYRELVIEGGAGTLEVPVTEDNIPGVEVAVLLIRGRVDVPGA
                     PPDQDLGRPAVAIGNVSLAVATTRKKIDLRLIPERSEVAPKETLKLEIQARDPSGAGQ
                     KASVAIMVVDEGVLSLMAFKTPDPLAFFHRARAPGVSLFDLRQYLLARSEEDTLTTDE
                     RDNDKGRQKNGYLNGDAGGATSTGSLGLVGTGRGGGGTGEGADMPAPPMPTAARSAAP
                     MAEMADEEAKPTKAAEKKMARSVAPDALDASVAMSQPISLRTLFATTAYFNPEVVVGP
                     TGLATIEIPMPENLTSFRVMAVAVDPDRPDHFGSAETQVKVRKPIMLRPSLPRFANFG
                     DSFQASVMVDNQTDKPQAIIVGTRGTNVVLPGETTKPIEVPAGQSQEVRFPMAVDRVG
                     TM"
BASE COUNT          248 a          581 c          516 g          187 t
ORIGIN      
        1 cgccgtcgag gacaagggcc gctggtcgta caagtacgag accgtcgacg tgcccgccgg
       61 cagctgccag ctgacctccg acgtcgcccc tgcgacctgc gagctcgacg tcggcaaggc
      121 cggcacctac gtcgcccgcg ccgagatcaa ggacgcgaag gggcggcgcg cgctgacccg
      181 ccacacgctc tacgtgtacg gcaaggacgc ggtcgtgtgg gaccaggacc agcgccgcgt
      241 cgacctcgtg cccgacaagc gcagctacga gcccggcgag caggcgaccg tcctgctgcg
      301 ctcgcccttc gaccgcgcgc gcggcctcgc ggtgatcgag cgcgagggca tcgtcgagta
      361 ccgtgagctc gtgatcgagg gtggcgccgg cacgctcgag gtccccgtca ccgaggacaa
      421 catccccggc gtcgaggtcg cggtgctgct gatccgcggc cgcgtcgatg tccccggcgc
      481 gccgccggac caggacctcg gacgtcccgc cgtggcgatc ggcaacgtct cgctcgcggt
      541 cgcgaccacg cgcaagaaga tcgacctccg gctgatcccc gagcgcagcg aggtcgcccc
      601 caaggagacg ctcaagctcg agatccaggc ccgcgacccc tccggcgccg ggcagaaggc
      661 ttcggtggcg atcatggtcg tcgacgaggg cgtgctctcg ctgatggcct tcaagacccc
      721 cgacccgctg gccttcttcc accgcgcccg cgcgcctggc gtcagcctgt tcgacctgcg
      781 ccagtacctg ctcgcgcgca gcgaggagga cacgctcacg accgacgagc gcgacaacga
      841 caagggccgg cagaagaacg gctacctcaa tggcgacgcc ggcggcgcga cgtccaccgg
      901 cagcctcggc ctcgtcggca ccggacgtgg gggcggtggc acgggcgagg gcgccgacat
      961 gccggcccca ccgatgccca ccgcggcgag gtccgcggcc ccgatggccg agatggccga
     1021 cgaggaagcc aagcccacga aggcggccga gaagaagatg gcccgctccg tcgccccgga
     1081 cgccctcgac gcctcggtgg cgatgagcca gccgatctcg ctgcgcacct tgttcgcgac
     1141 gacggcctac ttcaaccccg aggtcgtcgt cggcccgacc ggcctcgcga cgatcgagat
     1201 cccgatgccg gagaacctga ccagcttccg cgtgatggcg gtcgcggtcg accccgaccg
     1261 gcccgaccac ttcgggtccg ccgagacgca ggtcaaggtc cgcaagccga tcatgctgcg
     1321 cccctcgctg ccgcgcttcg ccaacttcgg cgacagcttc caggcgtcgg tgatggtcga
     1381 caaccagacc gacaagccgc aagcgatcat cgtcggcacc cgcggcacca acgtggtgct
     1441 gcccggcgag acgaccaaac cgatcgaggt ccccgccggc cagtcgcagg aggtccgctt
     1501 cccgatggcc gtcgaccgcg tcggcaccat gc
//
LOCUS       DASWNJ010001296         1690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_271982, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001296 DASWNJ010000000
VERSION     DASWNJ010001296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271982"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..>1690
                     /locus_tag="VGB85_21705"
     CDS             27..>1690
                     /locus_tag="VGB85_21705"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY0136720.1"
                     /translation="MEGPEIQFAEAVIDNGRFGTRTVRFETGRLARQAAGSAVAYLDG
                     DTMLLSATTAGKHPKEQFDFFPLTVDVEERMYAVGRIPGSFFRREGRPSEDAILTCRL
                     TDRPLRPSFKKGLRNEVQIVSTVLSLDPTHLYDVLAINSAACSVLLSGLPFTGPVGAT
                     RVACVAGQWIAFPTTEELEGATFDMVVAGRMLDDGDVAIMMVEAEATREAVEVIAGGG
                     RMPTEDVVAEGLEAAKPAIAAVCKAQLELQAKSPKEPLDFPVFLDYEDDVLAAVTEAG
                     EADLTAAMSIAGKQEREAATDAAKATVQEALADRFEGREKEVSAAFRSLTKSVVRRRI
                     VKDKVRIDGRGLEDIRQLSAEVEVLPRVHGSALFERGETQIMGVTTLNMLRMEQMVDT
                     LNPENRKRYMHHYNFPPYSTGETGRVGSPKRREIGHGALAERALLPVLPSREDFPYAI
                     RQVSEALGSNGSTSMGSVCASTMSLLNAGVPLKAPVAGIAMGLVFEGGEYATLTDILG
                     AEDAFGDMDFKVAGTPQYVTALQLDTKLDGIPSNVLAAALQQARTAR"
BASE COUNT          258 a          645 c          562 g          225 t
ORIGIN      
        1 gggggcttcg acagaggagg tgtcccatgg agggacccga gatccagttc gccgaggccg
       61 tcatcgacaa cggccgcttc ggcacccgca ccgtccgctt cgagaccggc cgcctggcgc
      121 gtcaggccgc cggctccgcc gtcgcctacc tcgacggcga cacgatgctc ctgtccgcca
      181 cgacggccgg caagcacccc aaggagcagt tcgacttctt ccccctgacg gtcgacgtcg
      241 aggagcggat gtacgcggtc ggccgcatcc ccggctcgtt cttccgccgc gagggccgcc
      301 cgtccgagga cgccatcctc acctgccgcc tcaccgaccg cccgctgcgc ccgtcgttca
      361 agaagggcct gcgcaacgag gtccagatcg tctcgacggt gctgtcgctc gacccgaccc
      421 acctctacga cgtgctcgcg atcaacagcg ccgcctgctc ggtgctgctg tccggcctgc
      481 cgttcaccgg cccggtcggc gccacccgcg tcgcctgcgt cgccgggcag tggatcgcct
      541 tcccgaccac cgaggagctc gagggcgcga ccttcgacat ggtggtcgcc gggcggatgc
      601 tggacgacgg cgacgtcgcg atcatgatgg tcgaggccga ggccacccgc gaggccgtcg
      661 aggtcatcgc cggcggcggc aggatgccga ccgaggacgt cgtcgccgag ggcctcgagg
      721 ccgccaagcc cgccatcgcc gcggtctgca aggcccagct cgagctccag gccaagtcgc
      781 ccaaggagcc gctcgacttc cccgtcttcc tcgactacga ggacgacgtg ctcgccgccg
      841 tgaccgaggc cggcgaggcc gacctcaccg ccgcgatgag catcgccggc aagcaggagc
      901 gcgaggccgc caccgacgcc gccaaggcca cggtgcagga ggcgctcgcc gaccgcttcg
      961 agggtcgcga gaaggaggtc agcgccgcgt tccgcagcct gaccaagagc gtcgtgcgcc
     1021 gccgcatcgt gaaggacaag gtgcgcatcg acggccgcgg cctcgaggac atccgccagc
     1081 tctcggccga ggtcgaggtc ctgccgcggg tgcacggctc ggcgctgttc gagcgcggcg
     1141 agacccagat catgggcgtc accacgctga acatgctgcg catggagcag atggtcgaca
     1201 cgctcaaccc cgagaaccgc aagcgctaca tgcaccacta caacttcccg ccgtactcca
     1261 ccggtgagac cggtcgcgtc ggctcgccga agcgccgcga gatcggccac ggcgcgctcg
     1321 ccgagcgcgc cctgctgccg gtgctgccga gccgcgagga cttcccctac gccatccggc
     1381 aggtctccga ggcgctgggc tccaacggct cgacgtccat gggctcggtc tgcgccagca
     1441 cgatgtcgct gctcaacgcc ggtgtgccgc tcaaggcgcc ggtcgccggc atcgccatgg
     1501 gcctggtctt cgagggtggc gagtacgcca cgctcaccga catcctcggc gcggaggacg
     1561 cgttcggcga catggacttc aaggtcgccg gcaccccgca gtacgtcacg gcgctgcagc
     1621 tcgacaccaa gctggacggc atcccgagca acgtgctcgc cgccgccctg cagcaggccc
     1681 gcaccgcccg
//
LOCUS       DASWNJ010001297         2247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_405614, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001297 DASWNJ010000000
VERSION     DASWNJ010001297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405614"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..849
                     /locus_tag="VGB85_21710"
     CDS             49..849
                     /locus_tag="VGB85_21710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136721.1"
                     /translation="MTLTLDLFARKYLCLVAALTIGCGPGKSDTGDDDGTTGASGAST
                     TDTPTGDGPPQDVCATGTAKTQVAIDFGVPVVPCEDEICSDERKGPCTVSAITSEGPT
                     EVTLACDYEDLGPATAVLTLGLEPDDTLDLEVGAAVQLSYDSSSGFEVGGSSYLRITD
                     DRGLVLGRGDIQRYGGLSGGTQWAEDYLTGLALPLTATIASAGCPDDENLSVITVARD
                     ESSVTVAQGTTGLLASGDAWRVVVETASIRHDEIEEGDVRLTIVRVEQ"
     gene            936..1829
                     /locus_tag="VGB85_21715"
     CDS             936..1829
                     /locus_tag="VGB85_21715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136722.1"
                     /translation="MASSLNMITLKCACLAAALAAGCTLDKSTTDDDGTTDATAATQT
                     STTDIPTTGGEHPTSAPPNDGGTDSTAATSDTSTTSTTSTTSTTDPPPPVACETGAAT
                     TAFKVDWDVDLAPCETESCHAKRVSPCIVSEIVKKPGPTILVLECNNLETGPTTDMVD
                     VFPEPGGTIDLEVGAAVQFTYESQSHGEIGGWERVRVTDDRGLVFAAGRDRLYSFPDD
                     DTQWAAEYLAELASPLGASFVDCTGEPQGHGIEVTRKAASVIVGDGGAATLTADASWL
                     VVVEYASRQGQLELVILRVKP"
     gene            complement(1830..2120)
                     /locus_tag="VGB85_21720"
     CDS             complement(1830..2120)
                     /locus_tag="VGB85_21720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136723.1"
                     /translation="MLAGCATRSGRTDQAAPLVIEVPDVDLDAEVFVDGNYVGQVKAL
                     RVPETGPILLAPGVHRVEVRKPGRFPVQRTVTVGKAQRTPAVVRAELLEDPA"
BASE COUNT          375 a          832 c          746 g          294 t
ORIGIN      
        1 ccgggggagg ctcgtggacg cccgcggctt atcgagtatc ctggcccgat gaccttgacc
       61 cttgatttat tcgcccgcaa atatctgtgc ctcgtcgccg cgctcacgat cggatgtgga
      121 cccggcaagt ccgacaccgg cgacgacgac gggaccaccg gggccagcgg ggcctcgacc
      181 accgatacgc cgaccggcga cggtccgccg caagacgtgt gcgcgacggg cactgcgaag
      241 acgcaggtgg cgatcgactt cggcgtgccc gtcgtgccgt gtgaggacga gatctgctcg
      301 gacgaacgca agggaccctg caccgtcagc gcgatcacca gcgagggtcc caccgaggtc
      361 acgctcgcat gtgactacga ggacctcggt cccgcgaccg ccgtcctcac cctcggcctc
      421 gagccggacg acacgctcga cctcgaggtc ggcgccgcgg tccagctcag ctacgactcg
      481 tcgtccggct tcgaggtcgg cggctcgagc tacctgcgga tcaccgacga ccgcggcctc
      541 gtgctcggca ggggcgacat ccagcggtac ggcggcctct cgggcggcac ccagtgggcc
      601 gaggactacc tgaccgggct cgcgctgccg ctgaccgcca cgatcgccag cgcgggctgc
      661 ccggacgatg agaacctcag cgtgatcacg gtcgccaggg acgagtcgag cgtcaccgtc
      721 gcccagggca ccacgggcct gctcgccagc ggcgacgcct ggcgcgtcgt cgtcgagacg
      781 gcttcgatcc gccacgacga gatcgaggaa ggcgacgtca gactcacgat cgtccgtgtc
      841 gagcagtgac gcggcgagcc ggccgatata gatgccgaca tcgggccgcg gccatcgttc
      901 gtggacatcg ccggtccgtc ggctatcctg cgacgatggc ctcttcgctt aatatgatca
      961 ccttaaaatg cgcctgtctc gcggccgccc tggccgctgg atgcaccctc gacaagtcga
     1021 cgacggacga cgacggcacc acggacgcga ccgccgcgac ccagacatcg accaccgaca
     1081 taccgacgac gggcggcgag cacccgacca gcgcgccgcc gaacgacgga ggcacggact
     1141 ccaccgccgc gaccagcgac acctcgacca cctcgaccac ctcgaccacc tcgaccaccg
     1201 acccgccgcc cccggtcgcc tgcgaaaccg gcgcggcgac cacggccttc aaggtcgact
     1261 gggacgtcga cctcgcgccc tgcgagaccg agtcctgcca cgccaagcgg gtcagcccct
     1321 gcatcgtcag cgagatcgtc aagaaacccg ggccgacgat cctcgtgctc gagtgcaaca
     1381 acctggagac gggccccacg accgatatgg tcgacgtctt ccccgagccg ggcgggacga
     1441 tcgacctcga ggtcggcgcc gccgtccagt tcacctacga gagccagagc cacggcgaga
     1501 tcggcggctg ggagcgcgtg cgagtcaccg acgaccgcgg cctcgtgttc gccgcgggcc
     1561 gggatcggct ctacagcttc cccgatgacg acacccagtg ggccgccgaa tatctggcgg
     1621 agctcgccag cccgctcggc gccagcttcg tcgactgcac cggcgagccc cagggccacg
     1681 gcatcgaggt cacgcggaag gcggccagcg tgatcgtcgg tgatggcggc gcggcgaccc
     1741 tcaccgccga cgccagctgg ctcgtcgtcg tcgagtacgc gtcacgtcag ggccagctcg
     1801 agctggtgat cctgcgcgtc aaaccctgat caggccgggt cttcgagcag ctcggcgcgc
     1861 accaccgcgg gtgtccgctg cgccttgccg accgtcaccg tgcgctgcac cgggaagcgc
     1921 ccgggcttgc ggacctcgac gcggtggacg cccggcgcca gcaggatcgg gccggtctcg
     1981 ggcacgcgta gcgccttgac ctgaccgacg tagttgccgt cgacgaacac ctcggcgtcg
     2041 aggtcgacgt cgggcacctc gatcaccagc ggggccgcct gatccgtgcg accggagcgc
     2101 gtcgcgcagc ccgcgagcac gcacgcgagc gccgcgacga cgagcgcacg gcgtcgctca
     2161 cagcgcgggg gtcggggcag gcgaagcatt ctgtaagctg acatgcacga cattggactc
     2221 gacctctccg acgaccagcg cgacgcc
//
LOCUS       DASWNJ010001298         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_23916, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001298 DASWNJ010000000
VERSION     DASWNJ010001298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23916"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VGB85_21725"
     CDS             <1..423
                     /locus_tag="VGB85_21725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136724.1"
                     /translation="VFERTDFGPEGERAREELALAGSVVAALRARGGQGALAVEVRLP
                     TPGDPGAAVVAGSLALDAASVVRITEGVLGAWSAGRVQVELAPARAEASEAAQATVAA
                     ADGAGRVASWQGWLLAVALLGLGVSAGVSIERLRQRRG"
     gene            475..>2006
                     /locus_tag="VGB85_21730"
     CDS             475..>2006
                     /locus_tag="VGB85_21730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY0136725.1"
                     /translation="MSSSTLVYVGIDLGTTNSTAAVFDGDKVTLVRNAQGSPITPSVV
                     RIDARGNMTIGSRARRFLESDPDNTRGEFKRLMGTAQTIRFAASGAEKLPEQLAAEVL
                     RSLRKDIEDQLGFVPARAVISVPALFELPQSSATSEAARLAGFERVELLQEPIASALA
                     AGWTIADSSGSWLVYDLGGGTFDVSLLETRDGLLRVVGHDGDNFLGGRDFDWAIVDWI
                     LGELARKEGVTLSRAEPGHAATLRKIKLAAEEAKIELSRVEETSIALPGLTAGDQSFD
                     LDLVISRDTLEKLCSPAIERSLAVCRRLLAAHGVPDGALTRVVMVGGPSVMPVVRRYV
                     SAALGVPPTEGLDPMTLVAQGAAIYAATAGLEARPTALEPIRGCKMWLQYPAMSSDLS
                     PHVIGRLVDRAASGSGVPAEIRLTRADGLWDSPWTALDEEGAFVTAVSLLPRKPNVFR
                     LEGRNGGGGQILLDPPTLTIVQGLTISDPPLSRTIGVATAGDDVRVYFERGAPLPARR
                     TFT"
BASE COUNT          242 a          684 c          813 g          267 t
ORIGIN      
        1 gtcttcgagc gcaccgactt cgggcccgag ggcgagcggg cgcgcgagga gctggccctc
       61 gccggcagcg tcgtcgccgc gctgcgggcg cggggcgggc agggggcgct cgcggtcgag
      121 gtgcggctgc cgacgccggg agatccgggg gcggccgtgg tcgcggggtc gctcgcgctc
      181 gatgctgcga gcgtggtccg catcaccgag ggggtgctcg gggcgtggag cgccgggcgg
      241 gtgcaggtcg agctggcgcc ggcgcgggcg gaggcgagcg aggcggcgca ggcgacggtg
      301 gcggccgcgg acggggccgg gcgcgtcgcc tcgtggcagg gctggctgct ggcggtcgcg
      361 ctgctcggcc tcggcgtctc ggcgggggtc tcgatcgagc gcctgcgaca gcgccggggg
      421 tgacctggcg gggggacgtg gcgagccccg gcggcccctg tatgcttgcc ggcgatgagc
      481 agctcgaccc tcgtgtacgt cggcatcgac ctcggcacca cgaactcgac ggcggcggtg
      541 ttcgacggtg acaaggtgac cctggtgcgc aacgcccagg gctcgccgat cacgccgtcg
      601 gtcgtccgca tcgacgcccg cgggaacatg acgatcggtt cgcgggcgcg gcgcttcctc
      661 gagagcgacc cggacaacac gcgcggcgag ttcaagcgcc tgatgggcac cgcgcagacg
      721 atccgcttcg cggcctcggg cgcggagaag ctgccggagc agctcgccgc ggaggtgctg
      781 cgatcgctgc gcaaggacat cgaggaccag ctcggcttcg tgcccgcgcg cgcggtgatc
      841 tcggtgccgg cgctgttcga gctgccgcag tcgagcgcga cctcggaggc cgcgcggctc
      901 gccggcttcg agcgcgtcga gctgctgcag gagccgatcg cctcggcgct ggcggccggc
      961 tggacgatcg ccgactcgag cgggagctgg ctggtctacg acctcggcgg cggcaccttc
     1021 gatgtctcgc tgctggagac gcgcgacggc ctgctgcgcg tcgtcgggca cgacggcgac
     1081 aacttcctcg gcggtcgcga cttcgactgg gcgatcgtcg actggatcct cggcgagctg
     1141 gcgcgcaagg agggcgtcac cctcagccgc gccgagcccg ggcacgcggc cacgctgcgc
     1201 aagatcaagc tcgcggcgga ggaggcgaag atcgagctca gccgcgtcga ggagaccagc
     1261 atcgcgctgc cggggctcac cgccggcgac cagagcttcg acctcgacct cgtgatcagc
     1321 cgcgacacgc tggagaagct gtgcagcccg gcgatcgagc gctcgctggc ggtgtgccgg
     1381 cgcctgctgg cagcgcatgg cgtgccggac ggggcgctga cgcgggtcgt gatggtcggc
     1441 ggtccgtcgg tgatgccggt cgtgcggcgc tacgtcagcg cggcgctcgg cgtgccgccg
     1501 acggagggcc tcgacccgat gacgctggtc gcccagggcg cggcgatcta cgcggcgacg
     1561 gcgggcctcg aggcgcggcc gaccgcgctc gagccgatcc gcggatgtaa aatgtggttg
     1621 cagtacccgg cgatgtcgtc ggacctcagc ccgcacgtga tcggtcgcct ggtcgaccgc
     1681 gccgcctcgg gcagcggcgt gccggccgag atccgcctga cccgcgcgga tggcctgtgg
     1741 gacagcccgt ggacggcgct ggacgaggag ggggcgttcg tcacggcggt ctcgctgctg
     1801 ccgcgcaagc cgaacgtgtt ccgcctcgag ggtcgcaacg gcggcggcgg gcagatcctg
     1861 ctcgacccgc cgacgctgac gatcgtgcag ggcctgacga tcagcgatcc gccgctgtcg
     1921 cgcacgatcg gggtggcgac cgcgggggac gacgtgcgcg tgtacttcga gcgcggggcc
     1981 ccgttgccgg cccgccgcac cttcac
//
LOCUS       DASWNJ010001299         2369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_93603, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001299 DASWNJ010000000
VERSION     DASWNJ010001299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93603"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1803)
                     /locus_tag="VGB85_21735"
     CDS             complement(<1..1803)
                     /locus_tag="VGB85_21735"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0136726.1"
                     /translation="MPVNLLGTHPLGGTRFRIRGRLGAGSMGVVYEAHDRERDEVVAL
                     KTLLHAEANALYRFKREFRALADIHHRNLVALHELLVDEQQCFFTMERVVGCTFLEYV
                     RPEHGPATDDPTFEGLDPEGDTGDLGDTRVPGDDPDEVEESQPADSLADASLASDGSF
                     VTDATLARDLSLGRASLLDASLEPETPPTRAASEDRPTGGAVDLARLRNALGQLAEGV
                     VALHQAGIVHRDLKPSNVMVTEDGRVVILDFGLATELTRPGAPEAEEDGLTGTAAYMA
                     PEQVAHGRGVAASDWYAVGVMLYEALAGVRPYTGALLQVLLAKRQHDPPPLAQVAPHA
                     PHDLAMLCEMLLRRDPATRPGDADVLRLLGRATRDAGSSGSGQIPRGLDAPLVGRTAH
                     LQALRDAFAATARGQAVTVYVHGPSGAGKSALVRGFLDELTGQDPRPVILSGRCYLRE
                     SVPYKALDPVVDSLSRFLNAMPEEQARVLMPRDLWALARLFPVMQTVVDNLHGRGPAA
                     AVLDQVELRRRAFAALRELLGRLADRRRVVITIDDLQWADADSSALLDGLLRPPDAPA
                     LLLLASFRSEEIDHQPFLRELLAQTGTAARRELAV"
     gene            complement(1834..>2369)
                     /locus_tag="VGB85_21740"
     CDS             complement(1834..>2369)
                     /locus_tag="VGB85_21740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEY0136727.1"
                     /translation="VCKVHGFCVAGGTDMALCSDLLVIADDAKIGYPPARVWGVPTSA
                     LWAHRIGPERAKRLLFTGDCLSGKEAAAWGLAVESAPADQLDERFEILLQRIALMPVN
                     QLVMMKLLVNQTLGAQGLQATQILGTVFDGIARHTPEGYAFRQRAMDAGFRQAVRDRD
                     EPFGDHGMSTFKGAPKG"
BASE COUNT          330 a          773 c          964 g          302 t
ORIGIN      
        1 gacggcgagc tcgcggcgcg cggcggtgcc ggtctgggcc agcagctcgc gcaaaaacgg
       61 ctggtgatcg atctcctcgc tgcgaaagct ggcgagcagg agcagggcgg gggcgtcggg
      121 cgggcgcagc aggccgtcga gcagggcgct cgagtcggcg tcggcccact gcaggtcgtc
      181 gatcgtgatg acgacgcggc ggcggtcggc gaggcggccg agcagctcgc gcagcgccgc
      241 gaaggcgcgc cggcgcagct cgacctggtc gagcacggcg gccgcgggtc cacgcccgtg
      301 caggttgtcg acgacggtct gcatgaccgg gaacaggcgc gcgagggccc agaggtcacg
      361 cggcatcagt acgcgggcct gctcctcggg catggcgttg aggaagcgcg agaggctgtc
      421 gacgaccggg tcgagcgcct tgtacgggac cgattcgcgc aggtagcagc ggcccgagag
      481 gatgacgggc cgcgggtcct ggcccgtgag ctcgtcgagg aagccgcgga cgagggcgct
      541 cttgccggcg ccgctgggac cgtgcacgta cacggtgacg gcctggccgc gcgcggtggc
      601 ggcgaaggcg tcgcgcaggg cctggaggtg ggcggtgcgg ccgacgagcg gggcatcgag
      661 gccgcgcggg atctggcccg agccggagct tccggcgtcg cgggtggcgc ggccgagcag
      721 gcgcagcacg tcggcgtcac cggggcgcgt ggcggggtcg cgacgcagca acatctcgca
      781 cagcatggcg aggtcgtgcg gggcgtgcgg ggcgacctgg gccagcgggg gcgggtcgtg
      841 ctggcgcttg gcgagcagca cctgcagcag cgcgccggtg taggggcgca cgccggcgag
      901 ggcctcgtag agcatgacgc cgacggcgta ccagtcgctc gccgcgacgc cgcggccgtg
      961 cgcgacctgc tcgggcgcca tgtaagcggc ggtgccggtg aggccgtcct cctcggcctc
     1021 gggcgcgccg ggccgcgtga gctcggtggc gaggccgaag tcgaggatga cgacgcggcc
     1081 gtcctcggtg accatgacgt tgctcggctt gaggtcgcga tggacgatgc cggcctgatg
     1141 cagcgcgacg acgccctcgg cgagctggcc gagggcgttg cgcaggcggg cgaggtcgac
     1201 ggcgccgccg gtcgggcggt cctcggacgc ggcgcgcgtg gggggcgtct cgggctccag
     1261 cgaggcgtcg agcagcgagg cgcgcccgag cgagaggtcg cgcgcgagcg tggcgtcggt
     1321 cacgaaagat ccgtcgctgg cgagcgaggc gtccgcgagc gagtcggcgg gctggctctc
     1381 ctcgacctcg tcggggtcgt cgccgggcac gcgggtgtcg ccgaggtcgc cggtgtctcc
     1441 ctcggggtcg aggccctcga aggtcgggtc gtcggtggcg ggcccgtgct cggggcgcac
     1501 atactcgagg aaggtgcagc cgacgacgcg ctccatcgtg aagaagcact gctgctcgtc
     1561 gacgagcagc tcgtgcaacg cgacgaggtt gcggtgatgg atgtcggcga gcgcgcgaaa
     1621 ctcccgcttg aaccggtaga gggcgttggc ctcggcgtgc aggagggtct tgagcgcgac
     1681 gacctcgtcg cgctcgcggt cgtgggcctc gtagacgacg cccatgctgc cggcgccgag
     1741 acgaccccgg atgcgaaagc gcgtgccgcc gagcggatgg gtcccgagga ggttcaccgg
     1801 caccgggaag gataacgagt cgcgccggcc gcgtcagccc ttgggcgcgc ccttgaaggt
     1861 cgacatgccg tggtcgccga agggctcgtc gcggtcgcgc acggcctggc gaaagccggc
     1921 atccatggcg cgctggcgaa acgcgtagcc ctcgggggtg tggcgggcga tgccgtcgaa
     1981 cacggtcccg aggatctgcg tcgcctgcag gccctgcgcg ccgagggtct gattgaccag
     2041 cagcttcatc atgacgagct gattgaccgg catgagcgcg atccgctgga gcaggatctc
     2101 gaagcgctcg tcgagctggt cggccggcgc ggattcgacc gcgagacccc acgccgcggc
     2161 ctccttgccc gacagacagt cacccgtgaa cagcagccgc ttggcccgct cgggcccgat
     2221 ccggtgcgcc cacagggcag aggtcggcac gccccacacg cgcgccggcg gatagccgat
     2281 cttggcgtcg tccgcgatca cgagcaggtc ggagcacagg gccatgtcgg tcccacccgc
     2341 gacgcaaaaa ccgtgcacct tgcacacga
//
LOCUS       DASWNJ010001300         2860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_444959, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001300 DASWNJ010000000
VERSION     DASWNJ010001300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444959"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VGB85_21745"
     CDS             <1..498
                     /locus_tag="VGB85_21745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136728.1"
                     /translation="KVQTGDNVDEEKNRRAVYVISFEPPTGAWFPAAGAWKKAAGARP
                     RPKTIPPYPEKWKQHEEDERAKRGGGGGGGSSSGGGSSDSDGGSASDGGGSDDSGSGS
                     ARRPGLGEGGDSGQPYIGGADGPPPVEGEPGASKKGCNMHGEEMPGALIVALAGILRL
                     RRRRG"
     gene            572..1918
                     /locus_tag="VGB85_21750"
     CDS             572..1918
                     /locus_tag="VGB85_21750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136729.1"
                     /translation="MVRRCGLVVSMLVACTAPEPAAEVPAWLRDDGERAPADLPEKPG
                     DPRWRVLTSIPECDAHLAADPAKLGRTLEWRPCATGKPGCRELVPPYINSAFGQIDIS
                     LLAATTARGVQVLLFEPNGGPERQRISVGPLDGPPFIVFDLKSHGGKCSFVNALFAAD
                     SAAVALIREANIDHNETIWFAGPLRADPAWHEPAARILTPQTAVRTGETSLSGRSLVH
                     AHEEGVRRSDPASASFLPVQAAPGMHHGFAVGASYVYATGDRIVVAAAPGAPIPLYAS
                     PEGAVIGPAYLEGERFFWAQGYGKDQVGRFDRIELWTALATDDPGISPRAGSPTRRCT
                     SFATSTASRQGTACWPIGSSTSRPRSCGRSSSMSRAVSVASGDPAPMRRPTRCCSSTA
                     RSSACTSWTTAAAAIAGSVSTCSTRCEPRAATDTLRSCRSRRSSPPPTPPLRSGGC"
     gene            1858..2283
                     /locus_tag="VGB85_21755"
     CDS             1858..2283
                     /locus_tag="VGB85_21755"
                     /inference="COORDINATES: protein motif:HMM:NF025473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABA4-like family protein"
                     /protein_id="HEY0136730.1"
                     /translation="MSLASIFSAANTTAALGWLLMIFAPRWRITDRVVLSGALALGLA
                     ALYTALLIAHFGDGEGGFSSLDEVVKLFRQPAMVLTGWVHYLAFDLFIGAWEARDARR
                     RGVPHLALVPCLLLTFMFGPVGLLVYRGVRVLLGRGRRV"
     gene            2491..>2860
                     /locus_tag="VGB85_21760"
     CDS             2491..>2860
                     /locus_tag="VGB85_21760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136731.1"
                     /translation="MVLGLVGCSDDGSGSSGAASDSSGGIVTGGLTTGSDTDVAPTGT
                     TTPTTEGPGGNSMSGTDDTSATATMTTATDPTATDTTATEPGTGTETASDTTAAIDTE
                     TAGTSTGGGGDLCEGGTLCGQ"
BASE COUNT          382 a         1036 c         1029 g          413 t
ORIGIN      
        1 aaggtccaga ccggcgacaa cgtcgacgag gagaagaacc gtcgcgccgt gtatgtcatc
       61 agcttcgagc cgccgaccgg cgcgtggttc ccggcggccg gcgcctggaa gaaggccgcc
      121 ggcgcgcgcc cgcgcccgaa gaccatcccg ccctaccccg agaagtggaa gcaacacgag
      181 gaggacgagc gcgccaagcg tggcggcggc ggcggtggtg gtagcagcag cggcggtggc
      241 tcgtccgaca gcgacggcgg ctcggcctcc gacggtggtg gctccgacga cagcggctcg
      301 ggcagcgcgc gtcggcccgg cctcggcgag ggcggcgact ccggtcagcc ctacatcggc
      361 ggcgccgacg gcccgccacc cgtcgagggt gagccgggcg cgagcaagaa gggctgcaac
      421 atgcacggcg aggagatgcc gggtgcgctg atcgtcgcgc tcgccggcat cctccgcctg
      481 cgacgccgcc gcggctgatc gcgccctgtg catggcatgg cgcgcggtcc tgcccggacg
      541 ctccgctcgg cgtacgtgtt aacgtacgac gatggtgcgt cgctgcggac tcgtcgtgtc
      601 gatgctcgtc gcctgcaccg cgccggagcc ggccgccgag gtcccggcct ggctgcgcga
      661 cgacggcgag cgggcccccg cggacctgcc cgaaaagcca ggtgatccgc gctggcgggt
      721 gctgacctcg atccccgagt gcgacgccca tctcgccgcc gacccggcga agctcggtcg
      781 caccctcgag tggcgcccct gcgcgaccgg caaaccgggc tgccgcgagc tggtcccgcc
      841 gtacatcaac tcggcgttcg ggcagatcga catctcgctg ctcgccgcga ccacggcgcg
      901 cggcgtgcag gtcctgctgt tcgagcccaa cggcggtccg gagcgccagc gtatctcggt
      961 cgggccgctc gacggcccgc ccttcatcgt cttcgacctc aagagccacg gcggcaagtg
     1021 cagcttcgtg aacgcgctgt ttgccgccga ctcggccgcg gtcgcgctga tccgcgaggc
     1081 gaacatcgat cacaacgaga cgatctggtt cgcgggaccg ctgcgcgccg acccggcctg
     1141 gcacgagccg gccgcgcgga tcctgacgcc gcagacggct gtaaggaccg gcgagacctc
     1201 gctgtccggc cgctcactcg tccacgccca cgaggagggc gtacgccgct cggatcccgc
     1261 gagcgccagc ttcctgcccg tgcaggcggc cccgggcatg caccacggct ttgcggtcgg
     1321 cgcctcgtac gtgtatgcga ccggcgacag gatcgtggtc gcggcggccc ccggcgcccc
     1381 gatccccttg tatgccagcc cggagggggc cgtgatcggc ccggcgtacc tcgagggtga
     1441 gaggttcttc tgggcgcagg ggtacggcaa ggaccaggtc ggtcgcttcg atcgcattga
     1501 gttatggacc gcgctggcca ccgatgaccc cggcatttct ccgcgcgcag gctcaccgac
     1561 acggcgctgc acgtctttcg cgacctcgac ggcctcaagg caggggacgg catgctggcc
     1621 tatcggcagt tcgacgagca ggccaaggtc atgcgggcga tcgtcgtcga tgtcgcgggc
     1681 ggtcagcgtc gcgtctggag atccggcgcc gatgcgacgc cctacgaggt gctgttcgtc
     1741 gaccgcgagg agctcggcct gcacgtcatg gacgaccgcg gcagcggcta tcgccgggtc
     1801 cgtctcgacg tgctcgaccc gctgtgagcc gcgcgccgcg actgataccc tgcgctcatg
     1861 tcgctcgcgt cgatcttctc cgccgccaac accaccgctg cgctcgggtg gttgctgatg
     1921 atcttcgcgc cgcgctggcg gatcaccgac cgcgtggtgc tctcgggcgc gctcgcgctc
     1981 gggctcgccg cgctgtacac cgcgctgctc atcgcgcact tcggcgacgg cgagggcggc
     2041 ttctcctcgc tcgacgaggt cgtcaagttg ttccgccagc cggcgatggt cctgaccggc
     2101 tgggtgcact atctcgcctt cgacctgttc atcggcgcgt gggaggcccg cgacgcgcgg
     2161 cggcgcggcg tcccgcacct cgcgctggtg ccgtgcctgc tgctgacctt catgttcggc
     2221 ccggtcggcc tgctggtcta ccgcggcgtg cgggtcctgc tcggccgggg ccggcgcgtg
     2281 tgacgaagct cgtcgagctc gccgtggaca gcgcccgccg ccgctcgctt atgtgccggc
     2341 gcggggcagg ccgggctggc cgatcctgcg cgtcgacctg tgcatgggcc tgcgcgtgct
     2401 gtgcggctcg cgcccgttga tcgctggtgg cgtgcgtggt cccatgcggc catgcatggt
     2461 catcgtcttg gaataggctt gagtggttgg atggtgctcg gcctggtcgg ctgtagcgac
     2521 gacgggagcg gtagctcggg cgctgccagc gacagcagcg gcggcatcgt caccggcggg
     2581 ctcacgaccg ggagcgacac cgacgtcgcg ccgaccggca cgaccacgcc gacaaccgag
     2641 ggccccggcg gcaacagcat gtcgggcacc gatgacacct cggcgaccgc caccatgacg
     2701 acggcgaccg atcccacggc gaccgacacg accgcgaccg agcccggcac cggcaccgag
     2761 actgccagcg acacgaccgc cgcgatcgac accgagaccg cgggcaccag caccggcggc
     2821 ggcggcgatc tgtgcgaggg cggcaccctg tgcggccaga
//
LOCUS       DASWNJ010001301         2434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_444982, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001301 DASWNJ010000000
VERSION     DASWNJ010001301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444982"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(360..1076)
                     /locus_tag="VGB85_21765"
     CDS             complement(360..1076)
                     /locus_tag="VGB85_21765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136732.1"
                     /translation="MTRTSSIPLLTAFFCLSTLAGCGKEAPKVKKSGEEDAPSAADNV
                     KVELPPPPDFDEGKVAEKYEDGVWSVYGLRKELDANVKQGEAGVDIEVRGYVQDIYEP
                     PVCPEGQQCPPGKQPHVWITDKPDDKGKKRAMMVVNYAFTIPEYDVKRWKDVPTVMMA
                     KGQQYTFKGKFKRFSDTGFADARGLLDFVAYKDPKSGTEGWVAPPGAPWHPLEEAAEA
                     ERQKALIEKVNKNAAKNRGG"
     gene            1402..>2434
                     /locus_tag="VGB85_21770"
     CDS             1402..>2434
                     /locus_tag="VGB85_21770"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0136733.1"
                     /translation="MTIGCGDEPTMPADPGPASVEAAAEDVPVADTVVIALRRLPAAL
                     DPTAELDPWGQRVVDDLLFEGLTRRLPDAPWAEPALADRCEVEHEGRGVACRLRPGAR
                     FHDDQPVTIEDVLYSINLWLGPRGANMRQRYGLDDLKSVEAGSPPGAEGGPWVRIGFA
                     GRDPLVLERLAAIKVVPRARHAGPGRFSQKPIGTGPMALVSQTEERLIFARREPVPGR
                     ARQIELRVQADGAMALTALRRGEIHMLPEVAPAHVPRELGKPGMAARFSAFLLSPPRY
                     DLILYNLREGPQAGPRMRGALDLSIPRVEIATTVHGVAGLATAAPVDLTVPTAIDLVA
                     IAEARIDDAG"
BASE COUNT          328 a          861 c          845 g          400 t
ORIGIN      
        1 cgtcctcgca gagcccgtcg agctcgctgc gcgggtcacc gctcatgagc ccgcactcct
       61 gggcggcgct gacgaagtcc tcgcaggact cgacctccgg agacttgcac ccgggggcgc
      121 caacgagggt cgagacgaac acgacgacga tggagaggtg cttcacggct gaaagatttc
      181 ctacaccttt gccgcgggcg ccgccagagc cgagaaacgg cgtcacgggg gcgctggcga
      241 gcatccacga caaaaccgcg atgacccggt cgtgtggtcg tcgcggtttt gtgtcgtcga
      301 acagctcgat cggtggggct tgccggccgc ggtctcagcc gcggtctcag ccgccgcggt
      361 cagccgcctc ggtttttcgc ggcgttcttg ttgaccttct cgatcaacgc cttctggcgc
      421 tcggcctcgg cggcctcctc gagcgggtgc cacggcgcgc ccggcggggc gacccagccc
      481 tcggtgccgg acttcgggtc cttgtacgcg acgaagtcga ggagaccgcg ggcgtcggcg
      541 aagccggtgt ccgagaagcg cttgaacttg cccttgaacg tgtactgctg gcccttggcc
      601 atcatcacgg tcgggacgtc cttccagcgc ttgacgtcgt actccgggat cgtgaaggcg
      661 tagttgacga ccatcatggc ccgcttcttg cccttgtcgt ccggcttgtc ggtgatccac
      721 acgtggggct gcttgccggg ggggcactgc tggccctcgg ggcacaccgg cggctcgtag
      781 atgtcctgga cgtagccgcg gacctcgatg tcgacgccgg cctcgccctg cttgacgttg
      841 gcgtcgagct ccttgcgcag gccgtagacc gaccacacgc cgtcctcata cttctccgcg
      901 accttgcctt cgtcgaagtc gggcggcggc ggcagctcca ccttcacgtt gtcggcggca
      961 ctgggtgcgt cctcttcgcc cgactttttg accttggggg cttccttgcc acagcccgcg
     1021 agggtgctga gacaaaaaaa cgcggtgagc agggggatcg aagaagtgcg cgtcatcgcc
     1081 ggggttgacc tccaagctct ccagggtgga gagccgttcg catggagcct gtaacacgcg
     1141 cgcaagcggg ttttcaagtg ctcgggaaac gcgatagtag agatggacat gcccgccgcg
     1201 ccaggaagcg cgccgtccgt ggcgcagtac ttcgccgccc ggccgccctc atccatcgcc
     1261 agacggcccc tgcgggcccg tggtggaccc gcggtcaccg gcgggggcgg gtgcgaggcc
     1321 gcgcgcgcgc tggctttcgc gcccacgaac ccgccgcgga taaaatttct tcgtgcagcc
     1381 gcgctgctcg ccggcgcgat cttgacgatc ggctgcggcg acgagccgac catgccggcc
     1441 gatcccgggc ccgcgtcggt cgaggccgcg gccgaggacg tgccggtcgc cgacaccgtg
     1501 gtcatcgcgc tgcgccgctt gccggccgcc ctcgatccta cggccgagct cgacccgtgg
     1561 ggccagcgcg tcgtcgacga cctgctgttc gaggggctga cgcgccgcct gcccgacgcg
     1621 ccgtgggccg agccggcgct cgccgaccgc tgcgaggtcg agcacgaggg tcgcggtgtc
     1681 gcctgtcgcc tgcgccccgg ggcccgcttc cacgacgacc agccggtgac gatcgaggac
     1741 gtgctctaca gcatcaacct ttggctcggt ccacgcggcg caaacatgcg ccagcgttac
     1801 ggtctcgatg acctcaagtc ggtcgaggcc gggtcgccgc cgggcgccga ggggggcccg
     1861 tgggtgcgga tcggcttcgc tggccgcgac ccgctggtgc tcgagcgcct cgccgcgatc
     1921 aaggtcgtgc cgcgggcgcg tcacgccgga ccgggccgct tctcgcagaa gccgatcggc
     1981 acgggaccga tggcgctggt ctctcagacc gaggagcgcc tgatcttcgc ccgccgcgag
     2041 ccggtcccgg ggcgcgcgcg gcagatcgag ctgcgcgtgc aagccgacgg cgcgatggcc
     2101 ctaaccgccc tgcgacgcgg cgagatccac atgctccccg aggtggcccc ggcccatgtg
     2161 ccccgtgagc tcggcaagcc cggcatggcc gcccgcttct cggctttcct tttgagtccg
     2221 ccacgttacg atctgatcct ttataatctg cgtgagggac cgcaggcggg tccgcggatg
     2281 cgcggggcgc tcgacctctc gatcccgcgg gtggagatcg ccaccaccgt gcacggcgtc
     2341 gccggcctcg cgaccgcagc cccggtcgac ctgacggtcc cgaccgcgat cgacctggtg
     2401 gcgatcgccg aggcccggat cgacgatgcc ggac
//
LOCUS       DASWNJ010001302         1885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_588589, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001302 DASWNJ010000000
VERSION     DASWNJ010001302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588589"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VGB85_21775"
     CDS             <1..502
                     /locus_tag="VGB85_21775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136734.1"
                     /translation="AGSAEKAYALLQRTRLSSGHHYMMADGRGFFGVETSGQLKVLTQ
                     TGARAAHLHTNHCFDPVLRKRERVPKVSTTFRRLELASTLYVQQRPQTAEQLWAFLGS
                     HEGYPRSLCSHVDDASGDPSASRTCGMMVMRLVGGEVLAGAGCGQEAPPRKFTVTRWR
                     GLTEGA"
     gene            499..1785
                     /locus_tag="VGB85_21780"
     CDS             499..1785
                     /locus_tag="VGB85_21780"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0136735.1"
                     /translation="MSSGHSSNSSNSQMIRASELAAAQRSHLLATWSVQGAVETLTID
                     QARGARFHTIEHGWLWDLESQVYNVNIGHGNLHVQERMINQIREMPTAAPNAVLPIRA
                     ELGALLHRVTGLEKAFLATGGSEAVENAIKIARLVTGRSKVITRRSSYHGATLAVLGV
                     AGDARKQPFVRDLAPAYHIEDPYPFAPGSPSEWVRSAERVIEDAGPDKVAAILLEGFT
                     GTNGMQVPPGDFWPAIRALCDRHGILLIDDEIFSGFGRTGKWFAREHWGVKPDMMVLG
                     KGLTSGYAPLSAVMVSAAIAAHFDDHKLWCGLTQYAHPVSCAAAVGVIEVMEREGLVD
                     NSERIGAALHTRLTALARRPAVHGAIRDVRGLGLMRAVEFDRPVAQLHAILLAKGAYV
                     PYRDQTLFLCPPLCLSGAEMHTVADLLEDAVRLFLK"
BASE COUNT          285 a          639 c          691 g          270 t
ORIGIN      
        1 ggccggatcc gcggagaagg cgtacgcgct gctacagcgg acgcggctgt cgagtgggca
       61 ccactacatg atggccgacg ggcgcggctt cttcggcgtc gagacctcgg ggcagctcaa
      121 ggtcctgacg cagaccggcg ctcgggcagc gcacctgcac accaaccatt gcttcgaccc
      181 ggtgctgcgc aagcgcgagc gggtcccgaa ggtcagcacg acgtttcgcc ggctcgagct
      241 ggcgagcacg ttgtacgtgc agcagcggcc gcagacggcc gagcagctgt gggccttcct
      301 cggcagccac gagggttatc cgcgcagcct gtgctcgcac gtcgacgacg cgagcggcga
      361 cccctcggcc tcgcgcacct gcggcatgat ggtgatgcgg ctcgtcggcg gcgaggtgct
      421 cgcgggggcc ggctgcgggc aggaggctcc gccgcgcaag ttcacggtga cgcgctggcg
      481 tggcctgacg gagggcgcat gagtagtggt cacagcagca acagcagcaa cagccagatg
      541 atccgcgcga gcgagctcgc ggcggcccag cgatcgcact tgctggcgac gtggagcgtg
      601 cagggcgcgg tcgagaccct gacgatcgac caggcccgcg gggcccgctt ccacacgatc
      661 gagcacggtt ggctgtggga cctcgagagt caggtctaca acgtcaacat cgggcacggg
      721 aacctgcacg tgcaggagcg gatgatcaac cagatccgcg agatgccgac ggcggccccg
      781 aacgcggtgc tgccgatccg cgccgagctc ggggcgctgc tgcaccgcgt caccgggctc
      841 gagaaggcgt tcctggcgac cggcggcagc gaggcggtcg agaacgcgat caagatcgcc
      901 aggctcgtca ccggccgcag caaggtcatc acgcggcgca gcagctacca cggggcgacg
      961 ctcgcggtgc tcggcgtcgc cggggacgcg cgcaagcagc cgtttgtccg cgacctcgcg
     1021 cccgcgtatc acatcgagga tccgtatccg ttcgcgccgg gctcgccctc ggagtgggtg
     1081 cgcagcgcgg agcgggtgat cgaggacgcc gggcctgaca aggtcgcggc gatcctgctc
     1141 gagggcttca cgggcaccaa cggcatgcag gtcccgcccg gagacttttg gccggcgatc
     1201 cgggcgctgt gcgaccgcca cgggatcctg ctgatcgacg acgagatctt tagcggcttc
     1261 gggcgcaccg ggaagtggtt cgcgcgcgag cactggggcg tcaagccgga catgatggtg
     1321 ctcggcaagg gcctgacctc cggctacgcg ccgctgtcgg ccgtgatggt ctcggccgcg
     1381 atcgcggcgc acttcgacga ccacaagctg tggtgcggcc tgacgcagta cgcccacccg
     1441 gtcagctgcg cggcggcggt cggcgtgatc gaggtgatgg agcgcgaggg cctggtcgac
     1501 aacagcgagc gcatcggcgc cgccctgcac acgcgcctga ccgcgctcgc gcgtcgcccc
     1561 gcggtccacg gtgcgatccg cgacgtccgc gggctcgggc tgatgcgcgc ggtcgagttc
     1621 gatcgaccgg tcgcgcagct gcacgcgatc ttgctcgcca agggcgcgta cgtgccttac
     1681 cgcgaccaga cgctgttcct gtgcccgccg ctgtgcctct cgggcgccga gatgcacacc
     1741 gtcgccgacc tgctcgagga cgccgtgcgg ctgttcctca agtgagcggg tctctgcggc
     1801 ttatccggcc gagatcggga gcgtcagcac cggaccgccg tgcttcatct cgccggactg
     1861 cagctcgagg gtctggctga ggaac
//
LOCUS       DASWNJ010001303         1956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_24035, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001303 DASWNJ010000000
VERSION     DASWNJ010001303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24035"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..463)
                     /locus_tag="VGB85_21785"
     CDS             complement(8..463)
                     /locus_tag="VGB85_21785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136736.1"
                     /translation="MYLRRNKVRCGESRRTYLSIAHNVWWSGEGNKKAQSRPIVVASF
                     GVEDQVDVDLARDLVAAVERCAPKFQVRRGEGKDATMRIAQEIRKIEPFLKALASRKL
                     GLSEHLPPHPERGVILEALIRDKLSDPTESSIGIRPEAILSGIKGGASD"
     gene            complement(773..>1956)
                     /gene="asnS"
                     /locus_tag="VGB85_21790"
     CDS             complement(773..>1956)
                     /gene="asnS"
                     /locus_tag="VGB85_21790"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004244664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEY0136737.1"
                     /translation="EVVGELVASPAAGQRVELQATEVIVVGLADVSFPLQKKRHGFEF
                     LREIAHLRARTNTFGAVFRVRSALAQAVHQFFQDRGFAYVHTPIITAADAEGAGEMFR
                     VSTLDPANPPRIDGKPDGKVDYTQDFFSKPTYLTVSGQLNGETFAMGLSEIYTFGPTF
                     RAENSNTTRHVAEFWMIEPEMAWTDLDDDADLAEAFVKHLMTTALERCEDDLAFFNKQ
                     IDQGLIARLQHVLSSSFERMSYTEAVRRLEQSGQKFEFPVFWGANLQAEHERYLTETL
                     VGRPVFVTDYPTEIKAFYMRRNDDGKTVAAMDLLVPKIGELIGGSQREERLDVLLAAL
                     EHGKLSAADYSWYLDTRRFGTVPHSGFGLGFERLVMYVTGMENIRDVIPFPRTPRNCE
                     F"
BASE COUNT          300 a          633 c          664 g          359 t
ORIGIN      
        1 ggacgactca gtccgacgcg ccgcccttga tgccggacag gatcgcctcg gggcggatgc
       61 cgatcgagga ctcggtcggg tcgctcagct tgtcgcggat gagcgcctcg aggatcacgc
      121 cgcgctcggg gtgcggcggg aggtgctcgg agagcccgag cttgcggctc gccagcgcct
      181 tgaggaacgg ctcgatcttg cggatctcct gggcgatgcg catcgtcgca tccttgccct
      241 ctccgcggcg tacctggaac ttcggggcgc agcgctcgac ggccgccacg aggtcgcggg
      301 cgaggtcgac atcgacctgg tcctcgacgc cgaagctggc gaccacgatc gggcggctct
      361 gcgccttctt gttgccctcg cccgaccacc acacgttgtg ggcgatcgac aggtaggtcc
      421 tacgggactc gccacagcgg actttgtttc ttcgcaggta cataggggta atcctttcct
      481 cagaccaagc tcatcgttgt ttcggcactt gtgccggcgc ttttggacgc cagtcttggg
      541 agatccttca tgcctcgatc cttggcctgc atcccttcgt ctgcctcacc aaaagggccg
      601 ttgtgcccac gtttccaagg ctagcgtttg cgccagacgc ccgccagggg cttttcgaat
      661 tttcctcact atacccacgg ggatgctgtt cccggccagt ctaccggagg tccgcaggtg
      721 ccatcgcgga ctccagaccc cagccaggtg tccgtggctc cagtcaatgg ctctagaact
      781 cgcagttcct cggcgtgcgc ggaaagggga tcacgtcgcg gatgttctcc atccccgtga
      841 cgtacatgac gaggcgctcg aagccgaggc cgaagcccga gtgcggcacg gtgccgaagc
      901 gacgagtgtc gaggtaccac gagtagtcgg cggcgctgag cttgccgtgc tcgagcgcgg
      961 cgagcagcac atcgagccgc tcctcgcgct gtgagccgcc gatcagctcg ccgatcttcg
     1021 ggaccagcag gtccatcgcc gcgaccgtct ttccgtcgtc gttgcggcgc atgtagaacg
     1081 ccttgatctc ggtcgggtag tcggtgacga acaccgggcg gccgacgagg gtctcggtca
     1141 ggtagcgctc gtgctcggcc tggagattcg cgccccagaa caccgggaac tcgaacttct
     1201 ggccgctctg ctcgagccgg cgcacggcct cggtgtagct catgcgctcg aagctcgacg
     1261 acagcacgtg ctgcaagcgg gcgatgagac cctgatcgat ctgcttattg aaaaacgcga
     1321 ggtcgtcctc gcagcgctcg agcgcggtcg tcatcaggtg ctttacaaag gcctcggcga
     1381 ggtcggcgtc gtcgtcgagg tcggtccacg ccatctccgg ctcgatcatc cagaactcgg
     1441 cgacgtggcg cgtggtgttg gagttctcgg cgcggaaggt cggaccgaag gtgtagatct
     1501 ccgacaggcc catcgcgaag gtctcgccgt tgagctggcc cgagacggtc aggtaggtcg
     1561 gcttgctgaa gaagtcctgc gtgtagtcga ccttgccgtc gggcttgccg tcgatccgcg
     1621 gcgggttggc cgggtcgagc gtgctgacgc ggaacatctc gcccgcgccc tcggcgtcgg
     1681 cggcggtgat gatcggcgtg tgcacgtagg cgaagccgcg gtcctggaag aactgatgca
     1741 ccgcctgcgc gagcgcgctg cggacccgga acaccgcgcc gaaggtgttg gtgcgcgcgc
     1801 gcaggtgggc gatctcccgc aggaactcga agccgtggcg cttcttctgc agcgggaagc
     1861 tgacgtcggc gaggccgacg acgatcacct cggtcgcctg aagctcgacg cgctggcccg
     1921 cggccggcga ggcgacgagc tcgccgacga cctcga
//
LOCUS       DASWNJ010001304         3351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_554756, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001304 DASWNJ010000000
VERSION     DASWNJ010001304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554756"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1024)
                     /locus_tag="VGB85_21795"
     CDS             complement(149..1024)
                     /locus_tag="VGB85_21795"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0136738.1"
                     /translation="MDIHQLRTFVAVAREGSITRASELLHLSQPAVSAHIKAIEDTLG
                     LALFERTSRGMILTRDGERLLARAEGTLAAHRELMAEATRIKGQLTGQLRLGAASNLN
                     GDAPARLLKILSDRCPEVEVVLRHGTSVEVLTGVRNGSLDAGFYHEAGAVDHELAAIE
                     VGRFTVCVVAPPGMVTVGEPVDWPALAEKPWIYPTASACCGQTAEALFKIHRIRPRRI
                     LSVDREDMTRTLVAAGTGVGLLHADTCKEARARGEVELVFEAPTPVRVLFAHLASRAQ
                     DPLLTAAAAILREGS"
     gene            1097..2008
                     /locus_tag="VGB85_21800"
     CDS             1097..2008
                     /locus_tag="VGB85_21800"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0136739.1"
                     /translation="MSHFHPDLAFAARFLPRFSIGPRLLRIARRLMRDPAPPRSTPGV
                     SASLRQIPGAGTARVFVPTGVTGPMPALLWVHGGGFIFGTPAQDDALATTFARELGLI
                     VVAPSYRLAPEHPFPAAIDDLYATLAWLHTSAGELHIRPDRIAVAGASAGGGLAAGLA
                     LLARDRGQIPLAFQLLVYPMLDDRTVDRTIDGANHRLWTAGSNRFGWTSYLGRTPGGA
                     DVPVYAAPARAGTLAGLPPTWIGVGTFDLFHDEDVAYARRLADAGVPVELEVVPGVFH
                     GFDVLRRANVSRNFVAAQVAALRRALA"
     gene            complement(2017..2823)
                     /locus_tag="VGB85_21805"
     CDS             complement(2017..2823)
                     /locus_tag="VGB85_21805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEY0136740.1"
                     /translation="MSSEAPRVTVHVDSGVADVRLDRPDKRNGLDLAMFLALVATGER
                     LRDDPGVRCVVLSGAGKAFCAGLDWPAFLALGEAGTRRLLERGADSPANLAQRAAWVW
                     SELPVPVIAAIHGATLGGGLQLALACDLRIAAADTQLSAMEVRYGIIPDMTASQTLLR
                     LVRPDLARELIYTGRIVGAEEALRIGLVTRIAADPHAEALALARTIAGQSPQAIRAAK
                     RLCNAAPQLDVAASFRLETELQVGLLATPNQVEAVQAAVARRPPVFADPD"
     gene            2928..>3351
                     /locus_tag="VGB85_21810"
     CDS             2928..>3351
                     /locus_tag="VGB85_21810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136741.1"
                     /translation="MKSEGFNPYAPPQADITAVPGAPGLDYFEENGCLVVRSGSVLPP
                     LCVRTGEVTVGNLKTRTVRQIPAWTIWVFVLVSPIVGGILMLVMQKKFKVTMAIGDAA
                     RRRRRVVMVIGLVVAVGAFAGVMAFARFGVRLSPVTLFA"
BASE COUNT          444 a         1206 c         1200 g          501 t
ORIGIN      
        1 ttcggcgagc tgctcgcccg cgcgggtgac gacacccttt ttaaagcggc acgcgaggcg
       61 ctgactgcct cgttcgcccg ccatcacggc gccgtgtcga tgcacgcccg cgtccatatc
      121 gtcaccggca cccgtctctg aattcatctc aagagccctc gcgcaggatc gcggcggcgg
      181 cggtcagcag ggggtcctgg gcccggctcg ccaggtgcgc gaagaggacg cgcacgggcg
      241 tcggggcctc gaacaccagc tcgacctcgc cgcgggcccg cgcctccttg caggtgtcgg
      301 cgtgcagcag ccccacgccg gtccccgcgg cgacgagcgt gcgggtcatg tcctcgcggt
      361 cgacgctgag gatccgccgc ggccggatcc ggtgaatttt aaaaagggcc tccgcggtct
      421 gtccgcagca ggcgctcgct gtcggataga tccacggctt ctccgcgagg gccggccagt
      481 ccaccggttc gccgactgtc accattcccg gaggcgcgac cacgcatacg gtaaatctcc
      541 cgacctcgat cgccgcgagc tcatgatcga ccgcgccggc ctcatgataa aatccggcgt
      601 cgaggctgcc gttgcgcacg ccggtgagaa cctccacgga cgtgccgtgg cgcaggacga
      661 cctccacctc ggggcagcga tccgacagga tcttcaagag ccgcgcgggc gcgtccccat
      721 ttaaattgct cgccgcgccg aggcggagct ggccggtcag ctggcccttg atgcgcgtcg
      781 cctcggccat cagttcgcga tgggcggcga gcgtgccctc ggccctggcg agcaggcgct
      841 cgccgtcgcg ggtcaagatc atcccccgcg aggtgcgttc gaacagggcg agaccgagcg
      901 tgtcctcgat cgccttgata tgtgcgctca ccgcaggctg gctcaggtgc agcagctccg
      961 aggcgcgcgt gatgctgccc tcgcgggcca ccgcgacgaa ggtcctgagc tgatgtatgt
     1021 ccatcgtgtc gtccgctcgc gtcacgcaag cctgccagag aactcgcccc acggggtccc
     1081 gcccggagca cccaccatga gccactttca ccccgacctc gccttcgccg cccgcttctt
     1141 gccccgcttc tcgatcggcc cgcgcttgct gaggatcgcg cgacgattga tgcgtgaccc
     1201 ggcgccgccc cgcagcaccc cgggggtgag cgcctcgctg cgacagatcc cgggcgccgg
     1261 cacggcccgc gtcttcgtgc ccaccggcgt caccgggccg atgccggcgc tgctgtgggt
     1321 ccacggcggc ggcttcatct tcggcacacc ggcgcaggac gacgcgctcg cgacgacctt
     1381 cgcgcgcgag ctcggcttga tcgtcgtcgc gccgtcgtac cgcctcgcgc cggagcaccc
     1441 cttccccgcc gcgatcgacg acctgtacgc gacgctcgcg tggctgcaca ccagcgccgg
     1501 ggagttgcac atacgccccg atcgcatcgc cgtcgccggc gcgagcgcgg gtggtgggct
     1561 cgcggccggg ctcgcgctgc tggcccgcga ccgcgggcag atcccgctcg cgttccagct
     1621 gctcgtctat ccgatgctcg acgatcgcac cgtcgacagg accatcgacg gcgccaacca
     1681 caggctgtgg accgcgggca gcaaccgctt cggatggacc tcgtacctcg ggcgcacccc
     1741 gggcggcgcg gacgtgccgg tgtacgccgc accggcgcgc gccgggaccc tcgcgggcct
     1801 gccaccgacg tggatcggcg tcggcacctt cgacctgttt catgacgagg acgtcgcgta
     1861 cgcgcggcgc ctcgcggacg ccggggtgcc cgtcgagctc gaggtcgtcc cgggggtgtt
     1921 tcacggcttc gatgtcctgc gccgcgcgaa cgtctccagg aacttcgtcg ccgcccaggt
     1981 cgccgcgctg cggcgcgcgc tggcctgaaa cacccgtcag tcggggtcgg cgaacaccgg
     2041 cgggcggcga gcgaccgccg cctgcacggc ctcgacctgg ttcggggtcg ccagcaggcc
     2101 gacctgcagc tcggtctcga ggcggaacga ggcggcgacg tcgagctgcg gggccgcgtt
     2161 gcacaggcgc ttggcggcgc ggatcgcctg cggcgactgg ccggcgatcg tgcgcgcgag
     2221 cgcgagggcc tcggcgtgcg ggtcggcggc gatccgcgtg acgaggccga tccgcagcgc
     2281 ctcctcggcg ccgacgatac ggccggtgta gatcagctcg cgggcgaggt cggggcgcac
     2341 gagccggagc agcgtctgcg aggcggtcat gtcggggatg atcccgtaac ggacctccat
     2401 cgccgagagc tgggtgtcgg ccgcggcgat ccgcaggtcg cacgcgagcg cgagctggag
     2461 gccgccgccg agggtcgccc cgtggatcgc ggcgatgacc ggcaccggca gctcggacca
     2521 cacccacgcg gcgcgctggg ccaggttggc cggggagtcc gcgccgcgct cgagcagccg
     2581 gcgggtgccc gcctcgccga gcgcgaggaa ggccggccag tcgaggccgg cgcagaacgc
     2641 cttgcccgcg ccggagagca cgacgcagcg cacgccgggg tcgtcgcgca ggcgctcgcc
     2701 ggtagcgacg agcgcgagga acatcgcgag gtcgaggccg ttgcgcttgt ccggccggtc
     2761 gaggcggacg tcggcgacgc cggaatcgac gtggacggtg acgcggggtg cttcgctgga
     2821 catgccggcg agtgtaccgt gggcgccgcg acactgtgta tatttgcatc gtctcgccgg
     2881 acccggcgcg gttgacccgc ggggcatttt agtgcgatcc ctcgtggatg aagtccgagg
     2941 gtttcaaccc ctacgcgccg ccccaggccg atatcaccgc agtcccgggc gcccccgggc
     3001 tcgactattt cgaggagaac ggctgcctgg tggtgcgcag cggctcggtg ctgccgccgc
     3061 tgtgcgtgcg caccggtgag gtcacggtcg gcaacctcaa gacccggacc gtgcgccaga
     3121 tccccgcgtg gacgatctgg gtgttcgtgc tggtctcacc gatcgtcggc gggatcctga
     3181 tgctcgtcat gcaaaaaaaa ttcaaggtga cgatggcgat cggcgacgcg gcacgtcggc
     3241 gccggcgggt cgtcatggtg atcggtctgg tggtcgcggt cggagcgttc gccggcgtca
     3301 tggcgttcgc ccggttcggg gtccggctgt cgccggtcac gctcttcgcc a
//
LOCUS       DASWNJ010001305         3016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_516126, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001305 DASWNJ010000000
VERSION     DASWNJ010001305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516126"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3016
                     /locus_tag="VGB85_21815"
     CDS             <1..>3016
                     /locus_tag="VGB85_21815"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0136742.1"
                     /translation="LLPSLNYILGEQPPVAALGPIEAQNRFTLYFERFVAVFARSSHP
                     LVLFLDDLQWIDAASLALLRTVLLSDRMESLFFCGAYRDNEVSAAHPLMLLREELTTR
                     GIAIRDLVLAPLDLANLVELIGDSLKNPHCGALAEVVLRKTGGNPFFVKQFMRSLHEQ
                     GVLVHEPALGWQWNLRKIEALQYTANVVDLMSDAIRRLPAATQDALKLAATIGNRFDL
                     GMLATVSECSVDEAYGRLDRALHDGLIARRGEQLHFIHDKVQEAAYLLVPAAERPAVH
                     LRIGRLLRARLALDDRQGLFDVVNHFNSAAALLVDPDERLDIAALDLLAAEKAEESAA
                     FAGALRYLELGATLLPADAWDHHYELALAYATRTGVMQSLCDLHDPAIATLVACYAHA
                     RGRLDQTAVRRMIMNVQVLKNDLLAALDEGLVALRAFDIHLPPFPDDDALAAELQATL
                     ALLELHFARTLGPDPAARTDEAMLAALTDLPALHDPELAALQDVMQEMFAPCYFLATN
                     NFGITVMKLVQNALLHGTSRHSLYAYVNFGTYLCATLDVERGYLFGRAAVRLDQLQPD
                     NKSQSMLNNMWGAFVQHWKEPYARYKETLQQGLHVGLETGQYIWAFYNTVNSNTNSLL
                     RGAPLADVLAESRSYMPMWRLDKFNAITWMVGAIAQIAHNLSNPVAEPHRLQGEWIDV
                     STIYAEAKRINSRASMFFANFYQVLLGVFQGAHLHTARLCDDTDPSIVGIASWHGNPA
                     YHFYAGVSYALALAELAPEDAETRARWHANVVTYAARVAAWADLCPPNLRHRDLLLHA
                     ELRRLEGDPTGAAELYDAGIAAAASGGFLQDEALGNELCGRFYASLGKQTIARAYLAE
                     AQRRYARWGASEAVARLEREWSQLLPREPGRRAVTHRDSVNVELDVGSVLKASQALSG
                     EIVLPRLLERLLDIILENAGARRGVLLLREDEVLRVEAGRDHDTARPAVLRGVLLEDS
                     PDVPIRVIQYVARTEEDVVLAELPGEGPFAGDP"
BASE COUNT          443 a         1179 c          965 g          429 t
ORIGIN      
        1 agctgctgcc ctcgctcaac tacatcctcg gcgagcagcc gccggtcgcc gcgctgggac
       61 cgatcgaggc gcagaaccgg ttcacgctgt acttcgagcg cttcgtcgcc gtgttcgcgc
      121 gcagcagcca cccgctggtg ctgttcctcg acgacctgca atggatcgac gccgccagcc
      181 tcgcgctgct gcgcaccgtg ctgctctcgg accggatgga gtcgctgttc ttctgcggcg
      241 cgtatcgcga caacgaggtc agcgccgcgc acccgctgat gctgctgcgc gaggagctga
      301 cgacccgcgg gatcgcgatc cgcgacctcg tgctcgcccc gctcgacctc gccaacctgg
      361 tcgagctgat cggcgacagc ctcaagaacc cgcactgcgg cgcgctcgcc gaggtcgtgc
      421 tgcgcaagac cggcggcaac ccgttcttcg tcaagcagtt catgcgctcg ctgcacgagc
      481 agggcgtgct ggtccacgag ccggcgctcg gctggcagtg gaacctgcgc aagatcgagg
      541 cgctgcagta caccgccaac gtcgtcgacc tgatgtccga cgcgatccgc cggctgcccg
      601 ccgccaccca ggacgcgctc aagctcgccg ccacgatcgg caaccgcttc gacctcggca
      661 tgctcgcgac cgtcagcgag tgcagcgtcg acgaggccta cggacgcctc gaccgcgcgc
      721 tgcacgacgg cctgatcgcc cgccgcggcg agcagctgca cttcatccac gacaaggtcc
      781 aggaggccgc ctacctgctc gtgcccgccg ccgagcggcc cgcagtgcac cttcgcatcg
      841 gccgcctgct gcgcgcccgc ctcgcgctcg acgaccgcca gggcctgttc gacgtcgtca
      901 accacttcaa cagcgccgcc gcgctgctcg tcgacccgga cgagcgcctc gacatcgccg
      961 ccctcgacct gctcgccgcc gagaaggccg aggagtcggc cgccttcgcc ggcgcgctgc
     1021 gctacctcga gctcggcgcc acgctgctgc cggccgacgc gtgggaccac cactacgagc
     1081 tcgcgctcgc ctacgcgacc cgcaccggcg tgatgcagtc gctgtgcgac ctgcacgacc
     1141 ccgcgatcgc cacgctcgtc gcctgctacg cgcacgcccg cggccgcctc gaccagaccg
     1201 ccgtgcgccg catgatcatg aacgtgcagg tgctcaagaa cgacctgctc gccgccctcg
     1261 acgagggcct cgtcgccctg cgggcgttcg acatccacct gccgcccttc cccgacgacg
     1321 acgccctcgc ggccgagctc caggccacgc tcgcgctgct cgagctgcac ttcgcccgca
     1381 ccctcggccc ggaccccgcc gcgcgcaccg acgaggcgat gctcgccgcg ctgaccgacc
     1441 tgcccgcgct acacgacccc gagctcgccg cgctgcagga cgtgatgcag gagatgttcg
     1501 cgccctgcta cttcctggcg accaacaact tcggcatcac cgtgatgaag ctggtccaaa
     1561 acgccctgct ccacgggacc tcgcgccact cgctgtacgc ctacgtcaac ttcggcacgt
     1621 acctgtgcgc gaccctcgac gtcgagcgcg gctacctgtt cggacgtgcg gcggtgcggc
     1681 tcgaccagct ccagcccgac aacaagtcgc agtcgatgct caacaacatg tggggggcct
     1741 tcgtccagca ctggaaggag ccctacgccc gctacaagga gacgctgcag cagggcctgc
     1801 acgtcgggct cgagaccggc cagtatatct gggcgtttta caacaccgtc aactcgaaca
     1861 ccaacagcct gctgcgcggg gcgccgctgg ccgacgtgct cgccgagtcg cgctcgtaca
     1921 tgccgatgtg gcggctcgac aagttcaacg cgatcacctg gatggtcggc gccatcgccc
     1981 agatcgccca caacctgagc aacccggtcg ccgagccgca tcgactgcaa ggcgagtgga
     2041 tcgacgtgtc gacgatctac gccgaggcca aacggatcaa cagccgcgcc tcgatgttct
     2101 tcgccaactt ctatcaggtg ctgctcggcg tgttccaggg cgcccacctg cacaccgcgc
     2161 gcctgtgcga cgacaccgac ccgagcatcg tcggcatcgc cagctggcac ggcaaccccg
     2221 cgtatcactt ctacgctggc gtctcctatg cgctcgccct cgccgagctc gcgcccgagg
     2281 acgccgagac ccgcgcccgc tggcacgcca acgtcgtcac ctacgcggcc cgggtcgccg
     2341 cgtgggccga cctgtgcccg ccgaacctgc gccatcgtga cttgctgctg cacgccgagc
     2401 tgcgccgcct ggagggcgac ccgaccggcg cggccgagct gtacgacgcg ggcatcgccg
     2461 cggccgcgtc cggcgggttc ttgcaggacg aggcgctcgg caacgagctt tgcggccggt
     2521 tctacgcgag cctcggcaag cagacgatcg cccgcgccta cctggccgag gcgcagcgcc
     2581 gctacgcccg ctggggcgcg agcgaggcgg tggcccggct cgagcgcgag tggtcgcagc
     2641 tgctcccgcg cgagccgggg cgccgcgccg tcacccaccg cgacagcgtc aacgtcgagc
     2701 tcgacgtcgg ctcggtgctc aaggcctcgc aggcgctgtc cggcgagatc gtgctgccgc
     2761 ggctgctcga gcggctgctc gacatcatcc tcgagaacgc cggggcccgc cgcggcgtgc
     2821 tgctgctgcg cgaggacgag gtgctgcgcg tcgaggccgg ccgcgaccac gacaccgcgc
     2881 gcccggcggt gctgcgcggg gtcctgctcg aggacagccc ggacgtcccg atccgggtga
     2941 tccagtacgt cgcgcgcacg gaggaggacg tcgtgctcgc cgagctgccg ggcgagggcc
     3001 ccttcgccgg cgaccc
//
LOCUS       DASWNJ010001306         6645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_588643, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001306 DASWNJ010000000
VERSION     DASWNJ010001306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6645
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588643"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..1761)
                     /locus_tag="VGB85_21820"
     CDS             complement(235..1761)
                     /locus_tag="VGB85_21820"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEY0136743.1"
                     /translation="MTPAQRPSASFHARLWGLSALLLLACRPAPSSAPVVRGVPVAAP
                     PPELREPARPGRTVVQDGPVLPTREASRPVTTSAALPPELVEVAEKLTAASAGRRRGW
                     ERLGTMSDRFGHRLSGSPALERAIDWTLEVMRQDGLANVRREKVMVPHWVRGAERARV
                     TAPIDRPLVILGLGGTVGTKGPLRGEVAVVSSLAEVAEKGAALRGKIVVINQPMPAFD
                     PVQQETGYRVTVPIRTSGASEAAKVGAKAVLIRSVTASSLRTPHTGAVRYADGVAKIP
                     AAALTLEDAELLARLAARGKTTVELSLGARTLPDAVSGNAIAEIRGRELPDEVVVIGG
                     HIDSWDTGDGSTDDGVGCVMAMDAAAMLLELGLRPRRTIRVVLFTNEENGLRGGFAYH
                     AAHGKEKHAGAIESDSGAGAPLGFGIAGTPEQVTAMQAYAPLFQGLGASQIAAGGGGA
                     DISPLMKDGVLGVGLEPDTSAYFDLHHSPADTFDKIDPDHMARNAAAMALMAYILAER
                     "
     gene            complement(1812..4223)
                     /locus_tag="VGB85_21825"
     CDS             complement(1812..4223)
                     /locus_tag="VGB85_21825"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0136744.1"
                     /translation="MRVAAKYRRLAAIGHGGMADVHLAVACGPGDFRKLLVLKELRPG
                     LAQDPEMQAMFRQEARIAGRMAHPNVVQTHEIGNDDSCAGGGRPFIAMEFLDGQSLQR
                     ILRRVRRDTGAGLPLPLHLRVLVELLAGLHHAHELCDYDGRPFGLVHRDVTPHNVIVT
                     HDGQVKLIDFGIARSLDGGADTQVGTFKGKASYCAPEQARGEPVDRRADLFAVGVMLW
                     EALTQRRMWPGLDEVAIVQRLRIGEVPALPDGPYSGLRALCRRTLAHDPAARPATAAE
                     LAEAIEQHAPGPRATQRQLAAIVSEHFAAERADLRALVEAQMRRPRERDPDAPALEIS
                     LFTTGNDNGPGALPGRVVDDPTRPDGLAARPRVTVPSGTSDEREEATESSPSRRIEPP
                     RTYGGGRRAVAAAFVALGLGAAGLSAQVYSDRSQGAGPKGQVCDATNGPEVELSGEIE
                     GDARLTCDKRYRLSFLTFVRPGATLTIDPGTTIVGDLATRGALVVQPGARLIAEGTAE
                     RPIVFTSAAPEGQRQAGDWGGLLLLGRAPINLQDAHGRSIRGRVEGIADGGEYGGDDP
                     NDSSGVLRHVRIEYSGVALGPNNEINGLTLAGVGRGTRVDHVQVRHSADDCFEFFGGT
                     VDASHLLCQDPGDDGFDWDLGYTGRLQFLLMRSAGGHGGHGLEGDNDPSGSDHAPVSA
                     PQIYNATLCGGDSRGHGLLLRHRSQATVGNAIFAGFAGGLEVRDGGSLRLPGGPVVGA
                     GELAAGPTVVDAALVGCDGASVVSTAGAVAPPDDGFFDASASFHGAVRDGQDAWARAA
                     WTRWD"
     gene            4393..5010
                     /gene="gstA"
                     /locus_tag="VGB85_21830"
     CDS             4393..5010
                     /gene="gstA"
                     /locus_tag="VGB85_21830"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF007831.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HEY0136745.1"
                     /translation="MKLYFSPGACSLSPHIALREAGLDFETERVDMATKKTRSGDDFT
                     TINPKGYVPALRLDNGEVLTEGPAIVQYIADLAPEKQLAPAAGTFERVRLQETLNFIS
                     TELHKGFSPLFRKDCPEEWKEVVRANLGRRFAALDAQLASQPFLLGEQFTIADGYLFT
                     VLGWTRFVNYDLAPYPNLVAYQARVAARPAVHAALEAEGLIKRGA"
     gene            complement(5024..5899)
                     /locus_tag="VGB85_21835"
     CDS             complement(5024..5899)
                     /locus_tag="VGB85_21835"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEY0136746.1"
                     /translation="MRAVLEAVAAVVERQRAALTAEFGWTQCDLTAHAEPGVLRLSGQ
                     VLLPRITTGLCACVAPLLPAGWRIDAGGVTVRPGLGWRALGPGVTPLWRTPRSAHVAA
                     DLTSELLASDGPVELLAERGGWALVRADDGTVGWLRGALRPGTRPPRRTSRVTPEVAL
                     RRLTARLRAHLGVQYRLGGTTRRHIDCSGLVQRCVREALGLVLPRHSTDQLACSVAAS
                     RSLGEPGDLLFMAGLDAAVCHVGVVLRGPRPGTRTLLHASSRRGRVVEEPLDRCLARA
                     SRVQHMELAQLLELR"
     gene            complement(5896..>6645)
                     /locus_tag="VGB85_21840"
     CDS             complement(5896..>6645)
                     /locus_tag="VGB85_21840"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY0136747.1"
                     /translation="RRLWFGVASRGPALREEDLAARSVEVTPIGTRVILEPSPLAEAL
                     GGVIDLRLVGEVFAENALAAACATHAVGIDASAIVRGLAACPGVPGRFELFAREGPVV
                     AVDYAHTPDALVRTCATARVLAGSGRVIVVFGAGGDTDAGKREPMGEAVGAAADLAIV
                     TTDNPRHEDPRAIAAMLIAGIDRGGRARVRVEPDRARAIALALQEAGRRDVVVIAGKG
                     HETGQTIGDETLPFSDREQLRALLGLPAEDA"
BASE COUNT          923 a         2672 c         2216 g          834 t
ORIGIN      
        1 ctcggcaccg tcgagatcgc cgacgacggc accggcgtgt tcatgaagac cgacctgtgg
       61 tcgatcggca ccggcgaggc caacgacgtc gtcgaccact cgatcatcat ccacgccgaa
      121 gccagcggcg gcacccgcat cgcctgcggc gtgatcgcca aggactgaaa aacgcagcgg
      181 cggcgactcg ccgacgaccg cgagacccgg caacggcgcc tcgcgccgcc cgcctcagcg
      241 ctcggccagg atatacgcca tcaacgccat cgcggccgcg ttgcgggcca tgtggtccgg
      301 gtcgatctta tcaaacgtgt cggcggggct gtggtggagg tcgaagtagg cgctggtgtc
      361 gggctcgagg ccgacgccga gcacgccgtc cttcatcagc gggctgatgt ccgcaccgcc
      421 gccgccggcg gcgatctgcg acgcgccgag gccctggaac agcggcgcgt aggcttgcat
      481 cgcggtgacc tgctccggcg tgccggcgat gccgaagccg agcggagcgc ccgcgccgga
      541 gtcggactcg atcgcgccgg cgtgcttctc cttgccgtgc gccgcgtgat acgcgaaccc
      601 gccgcgcagg ccgttctcct cgttggtgaa cagcaccacg cggatcgttc gtcgcggcct
      661 caggccgagc tcgagcagca tcgccgccgc gtccatcgcc atcacgcagc cgacgccgtc
      721 gtcggtgctg ccgtcgccgg tgtcccacga gtcgatgtgt ccgccgatca cgacgacctc
      781 gtcgggcagc tcgcgcccgc ggatctccgc gatcgcgttg ccgctgaccg cgtcgggcag
      841 cgtgcgcgcg ccgagcgaga gctcgacggt cgtcttgccg cgcgcggcca ggcgcgcgag
      901 cagctcggcg tcctcgagcg tcagcgcggc cgcggggatc ttcgccacgc cgtccgcgta
      961 gcgcaccgcg ccggtgtgcg gtgtccgcag gctcgacgcc gtgaccgacc ggatcagcac
     1021 cgccttcgcg ccgaccttgg ccgcctcgct cgcgcccgac gtgcggatcg ggaccgtcac
     1081 gcggtagccg gtctcctgct gcacgggatc gaacgcaggc atcggttggt tgatgacgac
     1141 gatcttgccc ctgagcgccg cgcccttctc ggcgacctcg gcgagcgagc tgacgaccgc
     1201 gacctcgccg cgcagcggcc ccttcgtacc gaccgtgccg ccgaggccga ggatcaccag
     1261 cgggcggtcg atcggcgcgg tcaccctggc ccgctcggcc ccgcggaccc agtgcggcac
     1321 catcaccttc tcgcggcgca cgttggccag accgtcctga cgcatgacct cgagcgtcca
     1381 gtcgatcgcg cgctcgagcg cgggcgaacc actgaggcgg tgcccgaagc ggtccgacat
     1441 cgtgccgaga cgctcccagc cgcggcgccg gcccgccgac gcggccgtca acttctccgc
     1501 cacctcgacc agctccggcg gcaacgcggc cgacgtcgtc accggccgcg acgcctcgcg
     1561 cgtcggcaac accggcccgt cctgcacgac cgtccgcccc ggccgcgccg gctcgcgcag
     1621 ctccggcggc ggcgcagcca cgggcacacc gcggaccacc ggcgcgctcg acggcgccgg
     1681 ccggcacgcg agcagcagca gcgccgacag gccccagaga cgcgcgtgga agctggcgga
     1741 cgggcgctgg gcgggagtca ccgcggagct atagcgcccc cgacggccgc gacgcaagcc
     1801 accgctcgcc atcaatccca gcgcgtccac gcggcccggg cccacgcgtc ctgcccgtcg
     1861 cgcacggccc cgtggaagct cgccgaggcg tcgaagaagc cgtcgtcggg cggcgccacg
     1921 gcgccggcgg tgctcaccac cgacgcgccg tcgcagccga ccagcgccgc gtcgacgacc
     1981 gtcggacccg ctgccagctc gccggcgccg accaccgggc ctccgggcag ccgcagcgaa
     2041 ccaccgtcac ggacctcgag cccgcccgcg aagccggcga agatcgcgtt gccgacggtc
     2101 gcctggctgc ggtggcgcag cagcagcccg tggccccgcg aatcgccgcc gcacagcgtc
     2161 gcgttgtaga tctgcggcgc gctgaccggc gcgtggtcgc tgcccgaggg gtcgttgtcg
     2221 ccctcgagac cgtgcccgcc gtggccaccc gcgctgcgca tcagcaagaa ctgcaggcgt
     2281 ccggtgtagc cgaggtccca gtcgaagccg tcgtcccccg ggtcctggca cagcaggtgg
     2341 ctcgcgtcga cggtgccgcc gaagaactcg aagcaatcgt ccgcgctgtg gcgcacctgc
     2401 acgtggtcga cgcgggtgcc ccgaccgacg cccgcgagcg tcagcccgtt gatctcgttg
     2461 ttgggcccga gcgcgacccc gctgtactcg atccgcacgt gacgcaggac cccgctgctg
     2521 tcgttcgggt cgtcgccgcc gtactcgccg ccgtccgcga tcccctcgac gcgcccgcgg
     2581 atcgacctgc cgtgcgcgtc ctggaggttg atcggcgcgc gccccagcag cagcagcccg
     2641 ccccagtcac cggcctggcg ctggccctcc ggcgccgcgc tggtgaacac gatcggccgc
     2701 tccgccgtgc cctcggcgat cagccgcgcg cccggttgca cgaccagcgc gccgcgggtc
     2761 gccaggtcgc cgacgatcgt cgtgcccgga tcgatcgtca gcgtcgcgcc cggtcgcacg
     2821 aaggtcagga agctcaggcg gtagcgcttg tcgcaggtca atcgcgcgtc gccctcgatc
     2881 tcgcccgaca gctcgacctc cggcccgttc gtcgcgtcgc agacctgtcc cttcggacct
     2941 gccccctggg acctgtccga atagacctgt gccgacagac cggcagcgcc gagccccagc
     3001 gccacgaacg cggccgcgac cgcccggcga ccgccaccat acgtccgcgg cggctcgatc
     3061 cggcgcgacg gcgaggactc ggtcgcctcc tcacgctcgt cggaggtgcc gctcggcacc
     3121 gtcacgcgcg ggcgcgcggc gaggccgtcc ggtcgggtcg gatcgtcgac cactcgcccc
     3181 ggcagcgccc cggggccgtt gtcgttgccg gtcgtgaaca gcgagatctc gagggccggc
     3241 gcgtcggggt cgcgctcgcg agggcggcgc atctgcgcct cgacgagcgc gcgcaggtcg
     3301 gctcgctcgg ccgcgaagtg ctcgctgacg atcgcggcca gctggcgctg cgtcgcccgc
     3361 ggtccgggcg cgtgctgctc gatcgcctcg gcgagctcgg ccgcggtcgc cgggcgggcc
     3421 gcggggtcgt gcgcgagggt ccgccggcac agcgcgcgca ggcccgagta cggcccgtcc
     3481 ggcagcgccg ggacctcacc gatccgcagg cgctgcacga tcgccacctc gtcgagcccg
     3541 ggccacatgc ggcgctgcgt cagcgcctcc cacagcatca cgccgaccgc gaacaggtcg
     3601 gcgcggcggt cgaccggctc gccgcgggcc tgctccggcg cgcagtagct ggccttgccc
     3661 ttgaacgtcc cgacctgggt gtcggcgccg ccgtcgaggc tgcgcgcgat gccgaagtcg
     3721 atcagcttga cctgcccgtc gtgcgtgacg atcacgttgt gcggcgtgac atcgcggtgg
     3781 accaggccga acgggcggcc gtcgtagtca cacagctcgt gcgcgtggtg cagcccggcg
     3841 agcagctcga cgagcacgcg caggtgcagc ggcagcggca gcccggcgcc ggtgtccctg
     3901 cggacgcgcc gcaagatccg ctgcagcgac tggccgtcga ggaactccat tgcgatgaac
     3961 ggccggcccc cgcccgcgca cgagtcgtcg ttgccgatct cgtgggtctg cacgacattg
     4021 gggtgggcca tgcgtccggc gatacgcgcc tcctggcgga acatcgcctg catctcgggg
     4081 tcctgcgcga gcccggggcg cagctccttg agcaccagca gcttgcgaaa gtcgccggga
     4141 ccgcaggcga ccgcgaggtg cacgtcggcc atgccgccgt gaccgatcgc cgcgagccgg
     4201 cgatatttcg ctgccaccct catcgagcgg aagctcccac acgaagtagg ccgcagcaag
     4261 cgcattcaca gcggcatgac gcacaccttg gtgctgacat ggtgcgcacc cgcgcgcaca
     4321 tttgcatccc cgggagaacc cgactggagc atgccttgtc acgcggacag cgttcatgcg
     4381 aagaggcaga ccatgaagct ctacttcagt cccggtgcct gctcactgtc ccctcatatc
     4441 gcgctgcgcg aggctggtct cgacttcgag accgagcgcg tcgacatggc gaccaagaag
     4501 accaggagcg gcgacgactt cacgacgatc aaccccaagg gctacgtccc ggcgctgcgc
     4561 ctcgacaacg gcgaggtgct gaccgaaggt ccggcgatcg tgcaatacat cgccgacctc
     4621 gcgcccgaga agcagctcgc cccggccgcc gggaccttcg agcgcgtgcg cctgcaagag
     4681 acgctcaact tcatctcgac cgagctgcac aagggcttta gcccgctgtt ccgcaaggac
     4741 tgcccggagg agtggaagga ggtcgtgcgc gccaacctcg gccgccgctt cgccgccctc
     4801 gacgcgcagc tcgcctccca gccgttcctg ctcggcgagc aattcacgat cgccgacggt
     4861 tacctgttca ccgtgctcgg ctggacccgc ttcgtgaact acgacctcgc gccctacccc
     4921 aacctcgtcg cctatcaggc ccgcgtcgcc gcccgcccgg ccgtgcacgc cgccctcgag
     4981 gccgagggcc tgatcaagcg cggcgcctga tctcgcccga gcatcagcgc agctcgagca
     5041 gctgcgcgag ctccatgtgc tgcacccgac tcgcgcgcgc gaggcagcgg tcgagcggct
     5101 cctcgaccac ccgcccgcgc cgcgacgagg cgtgcagcag cgtcctcgtc cccggcctcg
     5161 gcccgcgcaa caccaccccg acgtggcaca ccgcggcgtc cagccccgcc atgaacagca
     5221 ggtcgcccgg ctcgcccagc gagcggctcg ccgccaccga acacgcgagc tggtccgtcg
     5281 agtgccgcgg caacaccagc cccagcgcct cacgcacgca ccgctgcacc agccccgagc
     5341 agtcgatgtg ccgccgcgtc gtccccccga gccgatactg caccccgagg tgtgcacgca
     5401 gccgcgccgt cagccgccgc agcgccacct ccggcgtcac gcgcgacgtc cgtcgcggcg
     5461 gccgcgtgcc gggccgcaac gcccctcgca accagccgac cgtgccgtcg tccgcccgca
     5521 ccagcgccca cccgccgcgc tcggccagca gctcgaccgg gccatcactc gccagcagct
     5581 cgctcgtcag gtccgcggcg acatgtgccg acctcggtgt tcgccacagc ggcgtcacgc
     5641 cgggcccgag cgcgcgccag ccgagccccg gccgcaccgt caccccgcca gcgtcgatcc
     5701 gccagccggc cggcagcagc ggcgccacgc aggcacacag ccccgtcgtg atccgcggca
     5761 acaacacctg acccgacaat cgcagcaccc ccggctcggc gtgcgccgtc aggtcgcact
     5821 gcgtccaccc gaactcggcc gtcaacgcgg cgcgctggcg ctcgaccacc gccgcgaccg
     5881 cctcgagcac agctctcacg cgtcctccgc gggcaacccg agcagcgcgc gcagctgctc
     5941 ccgatccgag aacggaagcg tctcatcgcc aattgtctgc ccagtctcgt ggcccttgcc
     6001 ggcgatcacc acgacgtcgc ggcgaccggc ctcctgcaac gccagcgcga tcgcccgcgc
     6061 gcggtcgggc tcgacgcgca cgcgggcccg cccgccgcga tcgatccccg cgatcagcat
     6121 cgccgcgatc gcccgcggat cttcgtgtcg cggattatcg gtggtgacga tcgccaggtc
     6181 cgccgcggcc ccgaccgcct cgcccatcgg ctcgcgcttg cccgcgtccg tgtccccgcc
     6241 cgcgccgaac accacgatca cccgcccgct gcccgccagc acccgcgccg tcgcgcacgt
     6301 gcgcaccagc gcgtcgggcg tgtgtgcata gtcgaccgcg accaccggac cctcccgcgc
     6361 gaacagctcg aagcgccccg gcacccccgg acacgccgcc agcccgcgca cgatcgccga
     6421 ggcgtcgata ccgaccgcat gcgtcgcgca agcggccgcc agagcgttct ccgcgaacac
     6481 ctcgccgacg agccggaggt cgatcacccc gccgagcgcc tccgccagcg gcgaaggctc
     6541 gaggatcacc cgcgtgccga tcggcgtcac ctcgacgctg cgcgccgcga ggtcctcctc
     6601 gcgcagcgcc ggcccgcgcg acgcgacccc gaaccacagc cgccg
//
LOCUS       DASWNJ010001307         2211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_200595, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001307 DASWNJ010000000
VERSION     DASWNJ010001307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200595"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VGB85_21845"
     CDS             <1..681
                     /locus_tag="VGB85_21845"
                     /inference="COORDINATES: protein motif:HMM:NF036590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N C-terminal domain-containing
                     protein"
                     /protein_id="HEY0136748.1"
                     /translation="ALAEALRDPLLRVYEAGVPDTGSERMKVDARRLRNLALGYLVAL
                     GEPALVELAAKQFRDADNMTDGQEALAALIDHGGPARDAALAEFHARWAGDPLVLDKW
                     FTLQAISSHPDTLARVLALADHPDFNLLNPNRARSLLVSFGARNQFHFHDASGRGYAL
                     LADKVIALDPHNPHVAARLVAAFNSWRRFDAARQALMRTQLGRIAGQPGLSKSVGEIV
                     GRALAPAA"
     gene            706..1179
                     /locus_tag="VGB85_21850"
     CDS             706..1179
                     /locus_tag="VGB85_21850"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEY0136749.1"
                     /translation="MTPLRVTLVIALLGLLACKKSGGEQARPPEGDTSEQAADAGSDG
                     KLEDPEESPYLDVSNFNAMVESHHPEVVACWRETAGKAADAPKGRVKTTLVIDADGRV
                     KKVDFDAQRSTLKDPALDACIKEKASQWRFNISLTGADTPMPYTFDFSESGLLRG"
     gene            1374..>2211
                     /locus_tag="VGB85_21855"
     CDS             1374..>2211
                     /locus_tag="VGB85_21855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136750.1"
                     /translation="MPTSDPGTTGATTTADTGYTTTTDTDATSETGDSSTTTATTGTP
                     GICGDGLLDPGETCDLGFAGNSDEGACTQACQQAYCGDGLVWAGQELCDEGPNNNDQT
                     YNGCQRACTPGPRCGDGALQLEEECDPSAPQGENVVSCDPDNCRFNARVAFVTSLTFA
                     GDFGGLAEADAACTTAAANAGLDNASNFLAWLSDGVTGPAERFTIPAPDYPYARRDGQ
                     QLAYDLDTLYAQGLQVPLDITEHGTTLPPEEGAWTNTKFNGQPLSPQDHCQLWTSESS
                     LQL"
BASE COUNT          373 a          817 c          726 g          295 t
ORIGIN      
        1 gcgctcgccg aggccctgcg cgacccgctg ctgcgcgttt acgaggccgg cgtgccggac
       61 acggggagcg agcgcatgaa ggtcgacgcc cgccgcctgc gcaacctcgc gctcggatac
      121 ctggtcgcgc tcggcgagcc ggcgctggtc gagctcgcgg cgaagcagtt ccgcgacgcc
      181 gacaacatga ccgacggcca ggaggcgctc gcggccctga tcgaccacgg cggcccggcc
      241 cgcgacgcgg cgctggccga gttccacgcg cgctgggccg gcgacccgct ggtgctcgac
      301 aagtggttca cgctgcaggc gatctcgagc cacccggaca cgctcgcgcg ggtgctcgcg
      361 ctcgccgacc accccgactt caacctgctc aaccccaacc gggcgcgctc gctgctggtc
      421 agcttcggcg cgaggaacca gttccacttc cacgacgcca gcggccgcgg ctacgcgctg
      481 ctggccgaca aggtgatcgc gctcgacccc cataatcccc acgtcgccgc ccgcctggtc
      541 gccgccttca actcctggcg ccgcttcgac gccgcccggc aggcgttgat gcggacccag
      601 ctggggcgca tcgccggcca gcccggcctg tcgaagtcgg tcggggagat cgtcgggcgc
      661 gcgctggccc ccgcggcctg acgaccgcgt gataccgtcg cggacatgac tccgcttcgc
      721 gtcaccctcg tgatcgcgct cctcgggcta ctcgcgtgca agaagtccgg cggcgagcag
      781 gcccggccgc cggagggtga cacctccgag caggccgccg acgccgggag cgacggcaag
      841 ctcgaggacc ccgaggagtc gccctacctc gacgtctcca acttcaacgc gatggtcgag
      901 tcgcaccacc ccgaggtcgt cgcgtgttgg cgcgagaccg cgggcaaggc cgccgacgcg
      961 ccgaagggcc gcgtgaagac gaccctggtg atcgacgccg acggccgggt caagaaggtc
     1021 gacttcgacg cccagcgcag caccctcaag gaccccgcgc tcgacgcctg catcaaggag
     1081 aaggcgtcgc agtggcgctt caacatcagc ctcaccggcg ccgacacgcc gatgccctac
     1141 accttcgact tcagcgagtc ggggctgctc aggggctgac gtccggtcga gttgacaaca
     1201 gaagcgaaat cgcgcatcgt cgaggcgtgc cgggtagacc ccacgtcgcg tccatattcg
     1261 tcgtcgggct gctcgggccg gtaaccgcct gtcaggcgca cgccttgtcg cattcgacca
     1321 gcgagtccag ctcgagtgat gacgacggcg gacccggacc ggactcgccc cccgtgccga
     1381 cctcggatcc gggcacgacc ggcgcgacca cgaccgcgga caccgggtac accacgacca
     1441 cggataccga cgcgacgagc gagacgggtg actcgtcgac caccactgcg accaccggca
     1501 cgccggggat ctgcggagat ggcctgctcg acccgggaga aacttgcgat ctggggtttg
     1561 caggcaacag cgatgagggc gcttgtaccc aggcttgcca gcaagcttat tgtggcgatg
     1621 ggctcgtctg ggcgggccag gaattgtgcg acgaagggcc gaataacaac gatcagacgt
     1681 acaacggctg ccagcgagct tgtacgccgg ggcccagatg cggtgatggc gcgctacagc
     1741 tcgaagagga gtgcgaccca agcgcaccgc agggagagaa cgttgtttcc tgtgatcccg
     1801 acaactgccg cttcaacgcg agagtcgcgt tcgtcacgtc cctgaccttc gccggggact
     1861 tcggcgggct cgccgaggcc gacgctgcct gtaccacggc cgccgcgaac gccggcctcg
     1921 acaacgccag caacttcctc gcctggctca gcgacggcgt gaccggaccg gcggagcgct
     1981 tcactattcc ggccccagac tacccctacg cccgccgcga cggccagcag ctggcctacg
     2041 acctcgacac cctgtacgcc cagggcctgc aggtcccgct cgacatcacg gagcacggca
     2101 caaccctgcc gcccgaagag ggcgcctgga ccaacaccaa gttcaacggc cagccgctga
     2161 gcccgcagga ccattgccag ctctggacct cggagtcgtc gctacagctc g
//
LOCUS       DASWNJ010001308         7345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_163194, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001308 DASWNJ010000000
VERSION     DASWNJ010001308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7345
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163194"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1455
                     /locus_tag="VGB85_21860"
     CDS             <1..1455
                     /locus_tag="VGB85_21860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136751.1"
                     /translation="TGGEPIDTGTDPGSTTAVLPEPPVIVGFEVEPDLVEDNGLIQLA
                     VATKHADGVRMALDTGEVIELTAGQPASFTGQISAFTGLDNGEHIATLTPWREGVDDG
                     MSVPAPYYIALPDPGQQTAWETGDKIDSSGFVVAIAVLPDRRFVELGTYYLQGEPRCF
                     LRVRGLDNKWGAANMVSILPAYCTATDLTINPDTGALYVLVDRKGDNGVRWWLAEIPS
                     WGKGAKQIAIGSIGDKGLALARHPDMLAVCGAKKVATEDGIDAFAALVRPGQDVEERL
                     FDYTLNMMKHKFAETARDCAFADDTLVMVGEAWGEHSDKEMMQRDRLALLEYDVLTSI
                     GTWTVAGPNLGLQSRGLAVAIDDKKRYHLAGYNCLDDCKPNGDLWGYLPGGMLFTHTP
                     LGPLGSDAFGPHDIAWSPAGYMVIALGELSGQSYVFKVQAFAPEVPVPLWTFTPNDKQ
                     GLQIAYAVAVDPFGKVCAGGIGETNYPAFACMGS"
     gene            complement(1479..2624)
                     /locus_tag="VGB85_21865"
     CDS             complement(1479..2624)
                     /locus_tag="VGB85_21865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136752.1"
                     /translation="MRTRCPRLTLLAMVGACQGPTAESSGFSSTPAITTVGVGSTDTT
                     AGGTTGGSAGGSSTSSGGSTSGESGSGDTTVVLDLGTLPDAGDGNPVGCKGKIDLLFV
                     ISRDPNMEARQAKLIEAFPKFIETIEAKFEDFDYHIMVIDGDDEQWGSEFCNDDCPIF
                     DCEIGEECCPINAAPEKVGESCCSVEDYPCEYVDLVSDCDTKFGAGSVFPAGNYASNR
                     LCPIDGGRRYMVKGQKDLAETFACVAQIGVNGRGQLGEALTAAMHHYSNDPGGCNPGF
                     LRDDALLMVTFIQANPDHGGGGLESDGFAEDWAKAVLDAKHGDPESVVMLSFGTTYDW
                     GDYDEITRMAKMFPYGRYADLYETDFSAAFEEATGLVETACAVFVPG"
     gene            complement(2756..3106)
                     /locus_tag="VGB85_21870"
                     /pseudo
     CDS             complement(2756..3106)
                     /locus_tag="VGB85_21870"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            complement(3119..3460)
                     /locus_tag="VGB85_21875"
     CDS             complement(3119..3460)
                     /locus_tag="VGB85_21875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136753.1"
                     /translation="MRLHAILLSGLSLVAGCRAQAPGAPPVADAARPDPRDVTRPGDD
                     PPMYPASSDGPRIAITSNAYGAYRYAPDAPQLGDTLADFALPNARGGSYALREARAKG
                     PVVLVFYRGFW"
     gene            3568..5979
                     /locus_tag="VGB85_21880"
     CDS             3568..5979
                     /locus_tag="VGB85_21880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136754.1"
                     /translation="MTFRPLIGTFMICGAGLGLVACGDAAGTDDASSSGPGSSSGGDL
                     TEVTPTGDVPTTGDASSDATGDDSTTGDPAPVACNALPLPDALVDGGAWDPTLTIAGF
                     AGADGLNPGVHDFARDAEGHTLAVGYFQYLGVDRVPPFVRLAGDVWQAEPTLTIDLER
                     PTISAVAADELGNVAIATYSALPGDLAQRTGELLIREDGEFTLVGTFKGAVRRMAWYD
                     GELWVGGVYDLDGESVGPGLAVHGSMGWQDPPGGPLVGDGVYEVTVEGDELLIGGAFS
                     GVGGIAAQSVASWDGAEWTGFDLPEANVYALARDEQDQLYAGGLFSVEGSFTAGGVAR
                     WSGDGWVSMAGGLANSSFRGVVSDLVIHDGALTVAGCFASAGGAPDDPAAVPAQDLAR
                     WRDGAWEPLAEPGAGVGSVWFSPLKCGDEGPAAAWEMQYQRLFVDDERLLVGGFFPGV
                     AGTPSQSVLAREGDAWVPQGVAGRGFSGAPRSLAVGGPDCAAHVMGGTTHAGALAVDG
                     RVLRDDGDAWTPVGPPVPADMYCWQLAVDGAGAPVLACDGPQVGDDPPAGQVLRLVDD
                     AWQQVGDGFAQGGAGAVGFDPAGTLWAAGGGAEGYVARLVDEVFVVSGGFNGRVSALA
                     FRPTGAGQPVQVVAGGYFTELDGAPAGGVARWTGDAWETLGQGLSGSVLAVAYADDGS
                     IYASTADDGTPDRKILARWDGDTWSDVATPEPGPVPAGYAFYSLIARGKYVVAAGFAW
                     PGSGQRNVFVYDGESFTSLRGGVTAISVDAAVLAGDGLWFAGTVAEAGPPDARISSVG
                     VAHLE"
     gene            complement(6008..>7345)
                     /locus_tag="VGB85_21885"
     CDS             complement(6008..>7345)
                     /locus_tag="VGB85_21885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136755.1"
                     /translation="ALTIARGMLCDRVLQAGFDALDDEDFTQWMRRHGADERTLDSAL
                     CKAIYVLAFAYRDGREEQADIGAGTGLRTILRLTFGYKGSVFWLMQAGMGDVVFAPLY
                     LVLRRRGVRFEFFHRVERLRLDAAGRGVEAIEVAEQATPRGDYEPLLEVDGLPCWPCE
                     PHHDQLVEGAALRAGGVNLESWWAAWTDPRARVLQRGVDFDRVLLGIPVSCLRELCAE
                     IVDRDPRWRAMVDGLAAIQTQGVQLWLRGDAGALGWVMPRAVASNGDAPLNVWADMSH
                     LLAQERWGDGGEPPGHLLYFCGAMETAAHVPGRADHDFPAREAARTRESMRRWLLANT
                     ARTLPGAVDARGELAWDLLIDPGEGVGAGRLAAQVARANIDPTELYILSVRGSLRHRL
                     RCDESGLDNLVLTGDWLRNGLNIGCIESAVMSGMQAARALSGAPIVVVGEHDV"
BASE COUNT         1077 a         2579 c         2576 g         1113 t
ORIGIN      
        1 accggcggtg aaccgatcga taccggcacc gatcccggaa gcacgacggc ggtcctgccg
       61 gagccgccgg tgatcgtcgg cttcgaggtc gagcccgacc tcgtggagga caacggcctg
      121 atccagctcg cggtggcgac caagcatgcc gacggcgtcc gcatggcgct ggacaccggc
      181 gaggtgatcg agctgactgc cggccagccc gccagtttca ccggccagat ctccgccttc
      241 accgggctgg acaacggcga gcacatcgcc acgctgacac cgtggcgcga gggtgtcgac
      301 gatggaatgt ccgtcccggc gccctactac atcgcgctcc ccgatcccgg gcaacagacg
      361 gcctgggaga ccggcgacaa gatcgacagc agcgggttcg tcgtggcgat cgccgtgctg
      421 cccgaccgcc gtttcgtcga gctcggcacc tactatctgc agggtgagcc gcgctgcttc
      481 ctgcgcgtcc gcggcctgga caacaagtgg ggcgccgcga acatggtctc catcctgccg
      541 gcctactgca cggcgaccga cctgacgata aacccggaca ccggcgccct gtacgtgctc
      601 gtcgaccgca agggcgacaa cggggtgcgc tggtggctcg cggagatccc atcgtggggt
      661 aaaggtgcca agcagatcgc cattggatcg atcggcgaca agggcctcgc gctcgcgcgg
      721 cacccggaca tgctcgcggt ctgcggcgca aagaaggtgg cgacggaaga cggcatcgac
      781 gcgttcgcgg cgctcgtgcg accggggcag gacgtcgagg agcggctgtt cgattacact
      841 cttaatatga tgaagcacaa gttcgcggag accgcccgag actgcgcctt cgccgatgac
      901 acgctcgtga tggtgggcga ggcgtggggc gagcacagtg acaaggaaat gatgcagcgc
      961 gaccgcctcg ccctgctcga gtacgatgtg ctcacgagca ttggaacatg gaccgtcgcc
     1021 gggcccaatc tcggcctcca gagccgtggg ctcgccgtcg ccatcgacga caagaagcgc
     1081 taccacctcg ccggctacaa ctgcctcgac gattgcaaac caaacggtga cctctggggc
     1141 tacctgcctg gcggcatgct cttcacccac acgccgctcg gcccgctggg ttccgacgcg
     1201 ttcggcccgc acgacatcgc atggagcccg gccggctata tggtgatcgc actcggcgag
     1261 ctttcgggcc agtcctatgt gttcaaggtc caggcgttcg cccccgaggt tccggtgcca
     1321 ctgtggacct tcaccccgaa cgacaagcaa gggctacaga tcgcctacgc cgtggccgtc
     1381 gacccgttcg gcaaggtctg cgccggcggc attggcgaga ccaactaccc ggccttcgct
     1441 tgcatgggct cgtagcttac gcggccgccg aggcgcgatc aacccggcac aaacacggcg
     1501 cacgcggtct cgacgaggcc ggtggcctcc tcgaacgcgg cggagaagtc ggtctcgtac
     1561 aggtccgcgt atctgccgta cgggaacatc ttcgccatcc tggtgatctc gtcatagtcg
     1621 ccccagtcat acgtagttcc aaacgagagc atgaccaccg actcggggtc gccgtgcttc
     1681 gcgtcgagta cggcctttgc ccagtcctcc gcgaagccat cagattccag accaccaccg
     1741 ccgtggtccg ggttggcctg aataaaggtc accatcagca gcgcgtcgtc gcgcaggaag
     1801 ccagggttgc agccgccggg gtcgttgctg tagtgatgca tcgcggccgt gagcgcctcg
     1861 ccgagctggc cgcgcccgtt caccccgatc tgcgcgacgc aggcgaaggt ttcagcgagg
     1921 tccttctgac ccttgaccat gtaacggcgg ccgccgtcga tcgggcacag cctattggac
     1981 gcatagttcc cggcgggaaa cacgctgccg gccccgaact tcgtatcgca gtcggacacg
     2041 aggtccacgt actcacaagg ataatcctcg acactgcagc acgactcgcc gaccttttcg
     2101 ggcgcggcat tgatcgggca gcactcttcg ccgatctcgc agtcgaaaat cgggcagtca
     2161 tcgttgcaga attccgagcc ccactgctcg tcgtcaccgt cgatgaccat gatgtggtaa
     2221 tcgaagtcct cgaatttcgc ctcgatcgtc tcgatgaact tcgggaaggc ctcgatcagc
     2281 ttggcttgcc gcgcctccat gttcgggtcg cgcgagatca cgaacagcag gtcgatcttc
     2341 cccttgcagc ccaccgggtt gccgtccccg gcgtccggga gggtgccgag gtcgagcacc
     2401 accgtggtgt cgccggagcc ggactcgccc gaggtcgacc cccccgacga ggtcgagctg
     2461 ccgcccgcgc tcccgccggt cgtcccgccc gccgtggtgt cggtcgaccc gacaccgacc
     2521 gtcgtgatcg ctggtgtgct cgaaaagccc gatgattccg cggtcggccc ctggcaggcg
     2581 ccgaccatgg cgagcaacgt cagtcgcgga cagcgtgtcc tcatgaggcg cattttagcg
     2641 ccttttgcct cgcaagccag ccccgcgact tggcaggtga acgttcggac cgtgaacatc
     2701 ggtccagtca ttcacctgcg cctcaggtga atcgccagcg cgaccgattc atccgtcatt
     2761 tcgggcccga cttcgcgacc ggccttcgat acctgccctg atgatagtcg atcgcgtcga
     2821 gcagctcatt ggcgtaggcg cggcggcggg cgaccttgcg gtagaacacc ttgccgtggc
     2881 tatcgatgat gtagatcgcg tgcagcgaca gctcggggcg gtcctcgttg atgaggccga
     2941 atttgccgat cacgcgctgc tccgggtcgg cgagcaggtc gaaggtgatg cccttgcggt
     3001 cggcccacgg caccgtgtcg gcggggtcgt cgacgctgat gccgaccatg ctgaccccgc
     3061 ggtccgtgaa ttgtgtgctg aattcctgca ggtcaccgac ctgcgtgttg caagcgggtc
     3121 accagaagcc gcggtagaac acgaggacca cggggccctt cgcccgggcc tcgcgcaggg
     3181 cgtacgaccc gccgcgcgcg ttcggcagcg cgaagtctgc cagcgtgtcg ccgagctgcg
     3241 gcgcgtcggg ggcgtagcga tatgcgccgt atgcattgct ggtgatcgcg atccgcgggc
     3301 catcggagga cgcggggtac atcggtgggt cgtcgccggg ccgcgtcacg tcgcgcggat
     3361 ccgggcgggc ggcgtcggcc accggcggcg cgcctggtgc ttgcgcgcga cagcccgcga
     3421 ccagagacaa ccccgacagc aagatcgcgt gcagccgcat gatcaccact gtacccgggc
     3481 cgccgcctgt cacatcgctg cggggtggga tggacgcagg gggctccacc gactcggggc
     3541 tggccgtccg ggtgtatggc gaggggcatg acttttcgac ccttgatcgg caccttcatg
     3601 atctgcggcg cgggcctcgg cctggtcgcg tgcggcgacg cggccgggac cgacgacgcg
     3661 agcagcagcg gccccggcag ctcgagcggc ggcgacctca ccgaggtcac gccgaccggc
     3721 gacgtgccca ccaccggtga cgcgagcagc gatgcgaccg gcgacgacag cacgaccggc
     3781 gatcctgcgc cggtcgcctg caacgcgtta ccgctgcccg acgcgctcgt cgacggcggc
     3841 gcgtgggatc ccacgctcac gatcgccggc ttcgcgggcg cggacgggct caaccccggc
     3901 gtccacgact ttgcgcgcga cgcggagggc cacacgctcg cggtcggcta ttttcagtac
     3961 ctcggggtcg atcgggtccc gccattcgtg cggctcgcgg gtgatgtctg gcaggccgag
     4021 ccgacgctga cgatcgacct cgagcggccg acgatctcgg cggtcgccgc cgacgagctc
     4081 ggcaacgtcg cgatcgccac gtactcggcg ctgcccggcg acctcgcgca gcgcaccggc
     4141 gagctcctga tccgcgagga cggcgaattt acgctcgtcg gcacgttcaa gggcgcggtg
     4201 cggcggatgg cctggtacga cggcgagctg tgggtcggcg gcgtgtacga cctcgacggc
     4261 gagtcggtcg ggcccggcct cgcggtgcac ggctcgatgg gctggcaaga tcctccgggc
     4321 ggtccgctcg tcggtgacgg cgtgtacgag gtgacggtcg agggtgacga gctgctgatc
     4381 ggcggcgcat tttcgggcgt cggtgggatc gcggcccagt ccgtcgcgtc gtgggacggc
     4441 gccgagtgga ccggcttcga cctgcccgaa gccaacgtct atgcgctcgc gcgcgacgag
     4501 caggatcagc tgtacgccgg cggtctgttc tcggtcgagg ggtcgttcac ggccggtggg
     4561 gtcgcgcgct ggtcggggga cggatgggtg tcgatggccg gcggcctcgc caactcgagc
     4621 tttcgcgggg tcgtcagcga cctggtgatc cacgacggtg cgttgaccgt cgccggttgc
     4681 ttcgcgtcgg ccggtggtgc gcccgacgac cccgccgcgg tcccggccca ggacctcgca
     4741 cgctggcgcg acggcgcctg ggagccgctc gcggagcccg gcgccggcgt cggcagcgtg
     4801 tggttctcgc cgctcaagtg cggcgacgag gggcccgccg ccgcatggga gatgcaatac
     4861 cagcggctgt tcgtcgacga cgagcgcctg ctcgtcggcg gcttcttccc cggcgtcgct
     4921 ggcacgccct cgcagagcgt acttgcgcgc gagggcgacg cctgggttcc gcagggcgtg
     4981 gccgggcgcg gcttctcggg cgcgccgcgc tcgctggcgg tcggcggccc ggactgcgcc
     5041 gcgcatgtga tgggcgggac gacccacgcg ggcgcgctcg cggtcgacgg ccgggtgctg
     5101 cgcgacgacg gcgacgcgtg gacgccggtc ggcccacctg tccccgcgga catgtactgc
     5161 tggcagctcg cggtcgacgg cgccggcgcg ccggtgctcg cgtgcgacgg cccgcaggtc
     5221 ggcgacgacc cgccggccgg ccaggtgttg cggctcgtcg acgacgcctg gcagcaggtc
     5281 ggcgacggct tcgcgcaggg cggcgccggg gccgtcggct tcgaccccgc gggcacgctg
     5341 tgggccgcgg gcggcggcgc ggagggctac gtcgcgcggc tcgtcgatga agtcttcgtc
     5401 gtctcgggcg ggttcaacgg ccgcgtttcg gcgctcgcgt ttcgcccgac gggcgctggt
     5461 cagccggtgc aggtcgtcgc cggtggttac ttcacggagc tcgacggggc gcccgcgggg
     5521 ggcgtcgcgc gctggaccgg cgacgcctgg gagacgctcg gtcagggtct gtccggcagc
     5581 gtgctcgcgg tcgcctacgc ggacgacggc agcatatacg ccagcaccgc cgacgacggc
     5641 accccggacc gcaagatcct cgcgcgctgg gacggtgaca cgtggtccga cgtcgccacc
     5701 ccggagcccg gccccgtgcc ggccggttat gccttctact cgctgatcgc gcgcggcaag
     5761 tacgtggtcg cggccggctt cgcgtggccg ggctccgggc agcgcaacgt cttcgtgtac
     5821 gacggcgaga gcttcaccag cctgcgtggc ggcgtcactg cgatctcggt cgacgcggcc
     5881 gtgctcgcgg gcgacgggct gtggttcgcc ggcaccgtcg ccgaggccgg gccgcccgac
     5941 gcccgcatct cgagcgtcgg cgtcgctcac ctcgagtgag gcccggcaga gcgccggggc
     6001 gggcggctca gacgtcgtgc tcgccgacca ccacgatcgg cgccccgctg agcgcgcgcg
     6061 cggcctgcat gcccgacatc accgcggact cgatgcagcc gatgttgagt ccgttgcgca
     6121 gccagtcgcc ggtgagcacc aggttgtcga ggccggactc gtcgcagcgc aggcgatgac
     6181 gcaggctgcc acgcaccgag aggatgtaca gctcggtcgg gtcgatgttg gcgcgcgcga
     6241 cctgggcggc gagccgcccg gcgccgaccc cctcccccgg atcgatcagc agatcccacg
     6301 cgagctcgcc gcgcgcatcg acggccccgg gcaaggtgcg cgccgtgttg gcgagtaacc
     6361 agcggcgcat gctctcgcgc gtgcgcgcgg cctcgcgggc ggggaagtcg tgatcggcgc
     6421 gaccgggcac atgggcggcg gtctccatcg ccccgcagaa gtagagcagg tgcccgggcg
     6481 gctcgccgcc gtcgccccag cgctcctgcg ccaacagatg cgacatatcg gcccacacgt
     6541 tgagcggcgc gtcaccgttg ctggcgaccg cccgcggcat cacccagccg agcgccccgg
     6601 cgtcgccgcg cagccacagc tgcacgccct gggtctggat cgccgcgaga ccgtcgacca
     6661 tggcgcgcca gcgcgggtcc cgatcgacga tctccgcgca cagctcgcgc aggcagctga
     6721 ccgggatgcc gagcaacacg cgatcgaagt cgacgccgcg ctgcaacacc cgcgcccgcg
     6781 ggtcggtcca cgcggcccac catgactcga gattgacgcc gccggcccgc agcgccgcgc
     6841 cctccaccag ctggtcgtga tgcggctcgc aaggccagca cggcaggccg tcgacctcga
     6901 gcagcggctc atagtcgccg cgtggggtcg cttgctcggc gacctcgatc gcctcgacgc
     6961 cgcgccccgc ggcgtcgagg cgcaggcgct cgacccggtg gaagaattcg aagcgcacac
     7021 cgcggcggcg cagcaccaga tacagcggcg caaacacgac gtcgcccatg cccgcctgca
     7081 tcagccagaa caccgagccc ttgtaaccga aggtcaggcg caggatcgtg cgcagaccgg
     7141 tgcccgcgcc gatatcggcc tgctcctcgc gaccgtcccg gtaggcaaac gcgagcacgt
     7201 agatcgcctt gcatagcgca gagtccaggg tccgctcgtc ggccccgtgg cggcgcatcc
     7261 actgcgtgaa atcctcgtcg tcgagcgcat cgaagccggc ctgcagcacc cgatcgcaca
     7321 gcatgccgcg cgcgatcgtc agcgc
//
LOCUS       DASWNJ010001309         2494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_93733, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001309 DASWNJ010000000
VERSION     DASWNJ010001309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93733"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="VGB85_21890"
     CDS             <1..1263
                     /locus_tag="VGB85_21890"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0136756.1"
                     /translation="RAVGERRTTSTAAARGTTRAPVLWRFETGQAALGIFIDDELCWI
                     GNQRGDVYAIDHAGKVVRRFKLGDGVMCLVRDEDWLYCGCNDGKVYDLTRKVPFVAYE
                     ISDDLSIYWLDIHAGALAVSDANGGLTVLDHDCDLLWHKKSPGDSGWMVRCDDAGVYH
                     GYDDGLTAYGWSNGKKLWSQKISGCVMFGWQEATTLYAGTTGDEVLAFTKKGKQLRAY
                     KCDDSVYSCAAAGDGKYVFAGDSSSSIYCFDADGKRLWKLDTGAGSALSMQFFKDRLY
                     VVTSAGILACIDVSEAAIAAARQGTVPNAKLVAAGTAKAASVGDALEAADTGAEGVLV
                     ECYSEDGRVRVRPKSPGYKKLNVQFPRNIRTAGATYLVDGLRETAGGFYRVVGQIRTT
                     AGAASKTVAKKVSKTAAKKTTKKLSKRA"
     gene            1292..1897
                     /locus_tag="VGB85_21895"
     CDS             1292..1897
                     /locus_tag="VGB85_21895"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY0136757.1"
                     /translation="MPRSANHPVLLFASVLACAVPDDDLGVDPGEPTPVEYWLPLACG
                     VTARVGQGNASDFSHTGDATYAFDILLALDTPVHAMAAGTVLHIRADNDPGDPCHDGG
                     DESCFDFANLVVLLHADGTTTLYKHLNTVAVAEGSAVARGDVLGLSGSTGWSLTPHLH
                     AMRMEACDELQCPSIPLAFVDVPDGGVPITGQLVTSMNCRG"
     gene            1983..2162
                     /locus_tag="VGB85_21900"
     CDS             1983..2162
                     /locus_tag="VGB85_21900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136758.1"
                     /translation="MKDQLQQRLHQLQSEFESGQKVLRDLEVRQQEVRDTLLRIGGAI
                     QVLQELLKADDPAGT"
BASE COUNT          387 a          914 c          814 g          379 t
ORIGIN      
        1 cgcgctgtcg gcgagcggcg cacgacgagc accgccgccg cgcgcggcac cacccgggcg
       61 ccggtgctgt ggcgcttcga gaccgggcaa gccgcgctcg gcatcttcat cgacgacgag
      121 ctgtgctgga tcggtaacca gcgcggcgat gtctacgcga tcgatcacgc cggcaaggtg
      181 gtccggcggt tcaagctcgg cgacggtgtc atgtgtctgg tgcgcgacga ggactggctg
      241 tattgcggct gcaacgacgg caaggtctac gacctgacgc gcaaggtccc gttcgtcgcc
      301 tacgagatct cggacgacct cagcatctac tggctcgaca tccacgccgg cgcgctcgcg
      361 gtgtccgacg ccaatggtgg cctgaccgtg ctcgaccacg actgtgacct gctgtggcac
      421 aagaagtcgc ccggcgactc gggctggatg gtccgctgcg acgacgccgg cgtgtaccac
      481 ggctacgacg acggtttgac cgcctacggt tggtcgaacg gcaagaagct gtggtcgcag
      541 aagatctccg gctgcgtgat gtttggctgg caggaggcga ccacgctgta cgccggcacc
      601 accggcgacg aggtgctcgc cttcaccaag aagggcaagc agctgcgcgc ctacaagtgc
      661 gacgacagcg tgtactcgtg cgccgccgcg ggcgacggca agtacgtgtt cgccggcgac
      721 tcgtcctcgt cgatctactg cttcgacgcc gacggcaagc gcctgtggaa gctcgacacc
      781 ggcgccggct cggccctgtc gatgcaattt ttcaaagatc ggctctacgt cgtcacctcc
      841 gccggcatcc tcgcctgcat cgacgtcagc gaggctgcga tcgcagcggc gcgccagggc
      901 accgtgccca acgccaagct cgtcgccgcc ggcaccgcca aggcggccag cgtcggcgac
      961 gcgctcgagg ccgccgacac cggcgccgag ggggtgctcg tcgagtgcta cagcgaggac
     1021 ggccgcgtcc gcgtgcgccc gaagtcgccc ggctacaaga agctcaacgt gcagttccct
     1081 cgcaacatcc gcaccgccgg cgccacctat ctggtcgatg gcctgcgcga gaccgccggc
     1141 ggcttctacc gcgtcgtcgg ccagatccgc acgaccgccg gcgccgcgag caagaccgtc
     1201 gccaagaagg tcagcaagac cgccgccaag aagaccacca agaagctcag caagcgggcc
     1261 tagacctgtc gcccgggaca gctcgccgga catgccacgc agcgcgaacc accccgttct
     1321 cctcttcgcc tccgtgctcg cctgcgccgt cccggacgac gacctcggcg tcgaccccgg
     1381 cgagcccacg ccggtcgaat actggctgcc gctcgcctgc ggcgtgacgg cgcgggtcgg
     1441 tcagggcaac gccagcgact tcagccacac tggcgacgcc acatacgcct tcgacatcct
     1501 gctcgcgctc gacactccgg tccacgcgat ggccgccggg accgtcctgc acatccgcgc
     1561 cgacaacgac cctggagacc cctgccacga cggcggcgac gagtcctgct tcgacttcgc
     1621 caacctggtc gtgctgctgc acgccgacgg caccaccacc ttatataaac atctcaacac
     1681 cgtcgccgtc gccgagggat cggccgtcgc gcgcggggac gtgctcggcc tctcgggttc
     1741 gaccggctgg tcgttgacgc cccacctgca cgcgatgcgg atggaggcgt gcgacgagct
     1801 ccagtgcccg tcgatcccgc tcgccttcgt ggacgtcccg gacggcggcg tgccgatcac
     1861 cggccagctc gtcacctcga tgaattgtcg cggctgatcg cccgcgctcg caaacacccg
     1921 tcgccgccgc gcgtccgggg ccaccgcgat tcgtggtact ttcgccgccc gcggaacatg
     1981 ccatgaaaga ccagctccag cagcgcctcc atcagctcca gtccgaattc gagtccggac
     2041 agaaggtcct tcgcgacctc gaggtccggc aacaggaggt gcgtgacacc ctgctccgga
     2101 tcggcggcgc gatccaggtg ctgcaggagc tcctcaaggc ggacgacccc gcgggcacct
     2161 gagcgcattc gccagccgcg cgagcgcggc cgctcacccc tgcgcggaca tgatgcattg
     2221 tgtatgcatt cgcccgcgtc gcgagcactt ccatggcaat ttccgcgtgc ggagctcgcc
     2281 gcggtttcgt ctacaatcgc cgcgcgttgg tgcggatgtg gggattgggt cgcggtgatc
     2341 gcaggcgtcg gggacgtgcg tcctggccgc tggcggccct gctcgtgctc gcgggctgcc
     2401 tcgtgccggg gctcgcccgc gccggggatt gcatcgccgc gcgggccctc gccggactct
     2461 cgcccgacga gcaccagacc ctcgccggcc tgtg
//
LOCUS       DASWNJ010001310         1507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_693577, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001310 DASWNJ010000000
VERSION     DASWNJ010001310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693577"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VGB85_21905"
     CDS             <1..844
                     /locus_tag="VGB85_21905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136759.1"
                     /translation="LTAGEGAPVWSEESPNLSLPQPFEPRAREQSEDAGELVLDVAAD
                     VARGETVRLGWGGGDPGLGEPHVVLQRDEGGSFTDVTSPSGWAGAVFDNSRYHMLTHY
                     DPIPQQNGAILPSRQHHWYVDFQVPLELPAGTYRLVATGTHWDGAMTQTYSVDSTPFV
                     VTTGKADLLTATLDGDTLTVVWTHTPPALETVETWPVAGYRLLDPEVGPADPATIRAP
                     LTLDFFVDDQPAGDPQQVAHTPGKGHVLDFAALGLPTQGLSVRAHLGADIVPAFVTAD
                     VTGG"
     gene            926..>1507
                     /locus_tag="VGB85_21910"
     CDS             926..>1507
                     /locus_tag="VGB85_21910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136760.1"
                     /translation="MPSVRPLVIAALLLACNTDGSDDTTTTTGITGVSTATMTSPTGN
                     ATDAMTSAPTTSGAATATTGASAPGETGMTGEPVTTDDSLNTTGLVATSEDPPPSVDL
                     PPPPPEGDYAAQVIPGDPTRLSVRKADKDNDLCTMITFLSPAEGSPLEYDVQLPATWF
                     VQGALVQQGAAGCLTFEGFVEEPIVAYSGNGTAT"
BASE COUNT          235 a          602 c          489 g          181 t
ORIGIN      
        1 cctcacggcc ggcgagggcg cgccggtgtg gagcgaggag tcgcccaacc tctcgttgcc
       61 gcagcccttc gagccgcgcg cgcgcgagca gagcgaggac gccggcgagc tcgtgctcga
      121 cgtcgccgcc gacgtcgcgc gcggcgagac cgtgcgtctc ggctggggcg gcggcgaccc
      181 gggcctcggc gagccgcacg tggtcctgca gcgcgacgag ggcggcagct tcaccgatgt
      241 caccagcccg agcggctggg ccggcgccgt gttcgacaac agccgctatc acatgctgac
      301 ccactacgac ccgatcccgc agcaaaacgg cgcgatcttg ccgtcgcggc agcaccactg
      361 gtacgtcgac ttccaggtcc cgctcgagct gcctgcgggc acctatcgcc tggtcgccac
      421 cggcacgcac tgggacggcg cgatgactca gacttacagc gtcgattcga cgcccttcgt
      481 ggtgacgacc ggcaaggccg acctgctgac ggcgacgctc gacggcgaca cgctgacggt
      541 cgtctggacg cacacgcccc ccgcgctcga gaccgtcgag acctggccgg tcgccggtta
      601 tcgcctgctc gaccccgagg tcgggcccgc cgaccccgcc acgatccgcg cgccgttgac
      661 cctcgacttc ttcgtcgacg atcagcccgc aggagatccg cagcaagtcg cgcacacgcc
      721 ggggaagggc cacgtcctcg acttcgccgc gctcggcctg ccgacccagg gcctcagcgt
      781 gcgcgcccac ctcggcgccg acatcgtacc cgccttcgtc accgccgacg tcaccggagg
      841 atgaagcgtg cgcgcgcatg cacgcatcgc acggcggtat ccccgaactg gacagccgcg
      901 cccgcagggg cccatgatcg caaacatgcc ctccgtgcgc cccctcgtga tcgccgccct
      961 gctcctcgcc tgcaacaccg atggatcgga cgacacgacg accaccacgg gcatcacggg
     1021 tgtgtcgacg gcgacgatga ccagcccgac cggcaacgcc accgacgcga tgacctcggc
     1081 cccgaccacg agcggcgccg cgacggcgac caccggcgcc tccgcgccgg gcgagacggg
     1141 catgacgggc gagccggtga ccaccgacga ctcgctcaac accaccggcc tggtcgccac
     1201 cagcgaggac ccgcccccga gcgtcgacct cccgccgccg ccgccggagg gtgactacgc
     1261 ggcgcaggtc atccccggcg acccgacgcg cctcagcgtg cgcaaggccg acaaggacaa
     1321 cgacctgtgc acgatgatca ccttcctcag ccccgccgag ggcagcccgc tcgagtacga
     1381 cgtccagctg cccgcgacgt ggttcgtgca gggcgcgctc gtgcagcagg gcgccgccgg
     1441 ctgcctcacc ttcgagggct tcgtcgagga gccgatcgtc gcctactccg gcaacggcac
     1501 cgccacc
//
LOCUS       DASWNJ010001311         2151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_306862, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001311 DASWNJ010000000
VERSION     DASWNJ010001311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306862"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VGB85_21915"
     CDS             complement(<1..409)
                     /locus_tag="VGB85_21915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136761.1"
                     /translation="MRRPAPLKLSTPLLPLLLLSVLGGCGSDPADSDTEASTGTAAGS
                     TGTATDTGAASFPTSTETPTTEAPTTDAGTGTGDTTGDDTGEPPAPVDLDPWRDAIMY
                     FVFVDRFANGDPGNDAPLGAPVDPEADYLGGDWK"
     gene            complement(667..1377)
                     /locus_tag="VGB85_21920"
     CDS             complement(667..1377)
                     /locus_tag="VGB85_21920"
                     /inference="COORDINATES: protein motif:HMM:NF020448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HEY0136762.1"
                     /translation="MLHIHCGDSAATRAHQATLPEVAGGHQILVWHDSSAVGPCALDF
                     AAHRQLRARWWNADPSRMQDPRELPRDRPLVLWFGPDPWEQLALVELLAGLPDATVDV
                     SLVPLDRGVGESSPARLPAHFARRRPAPAREPLRRLWADFCADDRSALAAAVDDLRGD
                     PQLPHLAPALARILADRRDQLTERRVRTLVAAGVHDLMDLMPRLHALEAPGHGAWYGD
                     AIVARLRDAALGQHATPR"
     gene            1430..>2151
                     /locus_tag="VGB85_21925"
     CDS             1430..>2151
                     /locus_tag="VGB85_21925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136763.1"
                     /translation="MTSHFASIGIALASSDDMQALVERVADEAEYVEVPGGVYLVWTG
                     GDGPELWLQCDAEDNLVGMNPHFAGPTRMKVGLVAEIDRPGQTGLDGAMYGWVNPPGD
                     DAESGDYPLVFDCPDAAMHAELELPCVVTVQLAAFAHHLAVWDDVADYDRERDGDDTQ
                     LSSQAFIPAGLLRPEGDEDPPPPVAYAIVTGHVLAAEVRENPLTGLKYQWAAIATHGG
                     TLDVVAEMSLVTREIREGSVVSG"
BASE COUNT          283 a          663 c          910 g          295 t
ORIGIN      
        1 ccttccagtc gccgccgagg tagtcggcct cggggtcgac cggggcgccg agcggggcgt
       61 cgttgccggg gtcgccgttg gcgaagcggt cgacgaacac gaagtacatg atcgcgtcgc
      121 gccaggggtc gaggtcgacc ggggccggcg gctcgccggt gtcgtcgccg gtggtgtcgc
      181 cggtgccggt gccggcgtcg gtcgtcgggg cttcggtcgt cggggtctcg gtggaggtcg
      241 ggaagctggc ggcgccggtg tccgtcgcgg tgccggtcga gccggccgcg gtcccggtgc
      301 tggcctcggt gtcgctgtcg gcggggtcgc tgccgcagcc gccgagcacg gacagcagca
      361 gcagggggag caggggcgtc gatagtttca agggtgcagg gcgtcgcatg ggggagatcc
      421 ttggtgcggg gccgggatgg ccgcgcgcag gatcgcagag tcgcggcaga tgttatgttg
      481 gccgtcttac ggagattgca gcgaatgatc ggcgacgact gacattgcta tctggggagt
      541 ttccgtgtga atgctggcac ccgtgaatga caggagaatt tgcccggggg tggtcgccac
      601 catgcaggct cacattcgcg cggacatcgc cggtcgtggt cgcgcgcagc gccgctgctg
      661 gcaggctcag cgaggagtcg cgtgctggcc gagggccgcg tcgcgcaggc gggcgacgat
      721 cgcgtcgccg taccacgcgc cgtgaccggg ggcctcgagc gcgtgcaggc ggggcatgag
      781 gtccatgagg tcgtgcacgc cggcggcgac gagcgtacgc acgcgacgct cggtgagctg
      841 gtcgcggcgg tcggcgagga tccgcgcgag ggcaggggcg aggtggggca gctgcgggtc
      901 gccgcggagg tcgtcgacgg cggcggcgag ggcggagcgg tcgtcggcgc agaagtcggc
      961 ccacaggcgc cgcagtggct cgcgggcggg cgcggggcgg cggcgggcga agtgagcggg
     1021 cagccgggcg ggcgagctct cgccgacgcc gcggtcgagc ggcacgaggc tgacgtcgac
     1081 ggtcgcatcg gggaggccgg cgagcagctc gacgagcgcg agctgctccc acgggtcggg
     1141 gccgaaccac agcacgaggg ggcggtcccg cgggagctcg cgcgggtcct gcatgcgcga
     1201 ggggtcggcg ttccaccagc gggcgcgcag ctggcgatgg gcggcgaagt cgagggcgca
     1261 ggggccgacg gcggagctgt cgtgccagac caggatctga tggccgccgg cgacctcggg
     1321 gagggtcgcc tggtgggccc gcgtggcggc actgtcgccg cagtggatgt gcagcatcac
     1381 gcgggcggcc ataccacggg aggggcgatt atgggctaag gtcgcgccgg tgacgagtca
     1441 cttcgcgagc atcgggatcg ccctggcctc ctcggacgac atgcaggcgc tggtcgagcg
     1501 tgtggccgac gaggccgagt acgtcgaggt gcccggcggg gtgtacctcg tgtggaccgg
     1561 cggcgacggg ccggagctgt ggttgcagtg cgacgcggag gacaacctcg tcggcatgaa
     1621 cccgcacttc gccgggccga cgcgtatgaa ggtcgggctg gtcgccgaga tcgatcggcc
     1681 ggggcagacg ggcctcgacg gggcgatgta cgggtgggtc aacccgccgg gcgacgacgc
     1741 cgagtcgggt gattatccgc tggtgttcga ctgcccggac gcggcgatgc acgccgagct
     1801 cgagctgccc tgcgtggtca cggtgcagct cgcagcgttc gcgcaccacc tggcggtgtg
     1861 ggacgacgtc gcggactacg accgcgagcg cgacggggac gacacgcagc tgtcgtcgca
     1921 ggcgttcatc cccgcggggc tgctgcgccc cgagggcgac gaggatccgc cgccgccggt
     1981 cgcctacgcg atcgtgaccg gccacgtgct cgccgcggag gtgcgcgaga acccattgac
     2041 cggcctcaag taccagtggg ccgcgatcgc gacccacggc ggcacgctcg acgtcgtcgc
     2101 cgagatgagc ctcgtgaccc gtgagatccg cgagggcagc gtcgtgtcgg g
//
LOCUS       DASWNJ010001312         3329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_405913, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001312 DASWNJ010000000
VERSION     DASWNJ010001312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405913"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2020)
                     /locus_tag="VGB85_21930"
     CDS             complement(<1..2020)
                     /locus_tag="VGB85_21930"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0136764.1"
                     /translation="MAAVPLAQAAPAGPQGTTRPEVAGTNGPTGEPEFPPSMQFAHTA
                     IGDTALALSPSVLLSGHADTRDGARDAPSAPPLSGEFGPYQIVRQVGAGGMGAVYEAI
                     DGRTGGRVAIKSLLRMGAGDLHRFKYEFRSMAGVGHPNLVTLYELVSHASTWCITMEF
                     VEGVDLCKALRQRGRAAQLRGLVRQLALAIEALHAHNLLHRDLKPSNVLVTPGDRVVV
                     LDFGLVAEISRTSLLTHGGGAPVHAGTPVYMAPEQCASQLATPASDWYALGVMLYEAV
                     TGRLPFRGTPAQIFQAKQTAAPPVPAIPDDPALAALIAALLRRDPVERAAAAHVLAWC
                     DGVSAPLLGPGASASHATGDPAGEGVPVPRARAPSQATGTPRRAALIARRAERAALEQ
                     AYLEAAAGRPTSVYLRGVSGAGKTALGQCFLADVAQTGEAVVLSGRCYEHESVPFKAF
                     DSVIDALTEHLLGYASEELGPLLGAHCAELARLFPVLERIPAVRASAREHAARLGRAP
                     ALPGAEHESRRRAFAALKRLLYTLALRRPLVLAIDDLHWSDVDSLHLLRELLAPPEVP
                     PLLLLAAYRSDWPGSAAARQELERIQLRVLPEQSVVQIELGLLPQADAEALARAMLRA
                     AGLPTHPWARTIAAESGGNAFMMSAVVRHLSHEPEVLRAARLGAGVGLD"
     gene            2206..3162
                     /locus_tag="VGB85_21935"
     CDS             2206..3162
                     /locus_tag="VGB85_21935"
                     /inference="COORDINATES: protein motif:HMM:NF013246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005254 - chloride channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HEY0136765.1"
                     /translation="MLVQSRRTWLSEIFTYQGTVLPRIWRRLLFVAGLTFALTLGHVE
                     LRQFMVRDVTPLPFTLTGFALSVFLGFRTTTAYNRFWEGRTLWGGLVNVSRNFTRQVQ
                     TWLAPPPGTALDEPATAALTDLRRRLVHHTIAFNHALRQRLRDEDDLDEYEVMLDPEL
                     RASIVADVNRPLAITSHMAGLLRGAYDAGLIHPQHVPVLEASLGVMTDLQGGCERIRS
                     TPLPYVYRVLVHQIVGFYCFSLPFGLIHTLQWFAPVVAVLVAYAFFGLDAIGEELDNP
                     FETDANDLPLAAITRGIEIDLRARLGETDLPAAAVPHHGLLL"
BASE COUNT          418 a         1256 c         1201 g          454 t
ORIGIN      
        1 cgtcgaggcc gacgcccgcg ccgagccgcg cggcccgcag cacctctggc tcatgggaca
       61 ggtggcgaac gacggccgac atcatgaacg cgttgccgcc cgactccgcg gcgatcgtgc
      121 gcgcccacgg gtgcgtcggc aggccggcgg cgcgcagcat cgcccgcgcg agcgcctcgg
      181 cgtccgcttg tgggagcaga ccgagctcga tctgcacgac gctctgctcg ggcagcacgc
      241 gcagctggat ccgctcgagc tcctgccgcg ccgcggcgct gcccggccag tcgctgcggt
      301 acgccgccag caacagcagc ggcggcacct ccggcggggc gagcagctcg cgcagcaggt
      361 gcaggctgtc gacgtcgctc cagtgcaggt cgtcgatcgc gagcaccagc ggccggcgca
      421 gcgcgagcgt gtagagcagg cgcttgagcg ccgcgaaggc ccggcgccgc gactcgtgct
      481 ccgcgcccgg cagcgccggc gcgcgaccga ggcgcgccgc gtgctcgcgc gcgctggccc
      541 gcaccgcggg gatccgctcg agcaccggga acagccgcgc cagctcggcg cagtgcgccc
      601 ccagcagcgg cccgagctcc tcggacgcgt agccgagcag gtgctcggtc agcgcatcga
      661 tcacgctgtc gaaggccttg aagggcaccg actcgtgctc gtagcagcgg cccgagagca
      721 ccaccgcctc gccggtctgc gcgacgtcgg cgaggaagca ctggccgagc gccgtcttgc
      781 ccgcgcccga gaccccgcgc aggtacacgc tcgtcggtcg ccccgcggcc gcctcgaggt
      841 aggcctgctc gagcgccgcg cgctcggccc ggcgggcgat cagcgcggcg cggcgcgggg
      901 tgccggtcgc ctgcgagggc gcgcgcgcgc ggggcaccgg cacgccctcg ccggctggat
      961 cgccggtcgc gtggctggcc gaagcgccag gtccgagcag cggggccgac accccgtcgc
     1021 accacgcgag cacgtgcgcg gccgcggccc gctcgaccgg gtcgcggcgc agcagcgcgg
     1081 cgatcagggc cgcgagcgcg gggtcgtcgg ggatcgcggg gaccggcggc gccgcggtct
     1141 gcttggcctg gaagatctgc gccggcgtgc cacgaaatgg caggcgaccg gtcaccgcct
     1201 cgtagagcat cacgccgagc gcgtaccagt cgctcgccgg ggtcgccagc tggctcgcgc
     1261 actgctcggg cgccatgtac accggcgtgc ccgcgtgcac cggggcgccg ccgccgtgcg
     1321 tcagcaggct ggtgcgcgag atctcggcga cgaggccgaa gtccagcacg acgacgcggt
     1381 cgcccggcgt caccagcacg ttcgacggct tgaggtcgcg gtgcaggagg ttgtgggcgt
     1441 gcagggcctc gatcgcgagc gcgagctggc ggaccagccc gcgcagctgg gccgcgcgcc
     1501 cgcgctggcg cagcgccttg cacaggtcga cgccctcgac gaactccatc gtgatgcacc
     1561 acgtcgacgc gtgcgacacc agctcgtaga gcgtgacgag gttggggtgg ccgacgccgg
     1621 ccatgctgcg aaactcgtac ttgaagcggt gcaggtcgcc ggcgcccatg cgcagcagcg
     1681 acttgatcgc cacgcgcccg ccggtgcgcc cgtcgatcgc ctcgtacacc gcccccatgc
     1741 cgccggcgcc gacctgccgc acgatctggt agggcccgaa ttcgcccgac agcggcggcg
     1801 cggacggggc gtcgcgcgcc ccgtcgcgcg tgtcggcgtg gccgctgagc agcacgctcg
     1861 gagacagcgc gagcgcggtg tcgccgatcg ccgtgtgcgc gaattgcatc gacggcggga
     1921 actcgggctc gcccgtcggc ccgtttgtgc ccgcgacctc cggccgggtc gtgccctgcg
     1981 gacccgccgg ggccgcctgg gccagtggta cggcggccac gctgctgcgc ccggcgtcgt
     2041 gcgcgggtac gcctggggac tgttgggggc gctcggaggg ccgcatcgcc gtactgcggt
     2101 caggctatca cgccccgcgc gcggtttcct gaccgctttt gcggcggccc gacgcccccg
     2161 accctggcgc atcacgccct ctcttgccgt atctttgccc ctcccgtgct cgtccagtcc
     2221 cgcagaacct ggctcagcga gatctttacc taccagggca ccgtgctgcc gcggatctgg
     2281 cggcgcctgc tgttcgtcgc cggcctgacc ttcgcgctga ccctgggcca cgtcgagctg
     2341 cgccagttca tggtccgcga cgtcaccccc ctgcccttca cgctgaccgg cttcgccctg
     2401 agcgtgttcc tcggctttcg cacgacgacc gcctacaacc gcttctggga gggccgaacg
     2461 ctgtggggcg gcctggtcaa cgtctcgcgc aacttcaccc gtcaggtgca gacctggctc
     2521 gccccgccgc cgggcaccgc gctcgacgag ccggcgacgg ccgcgctgac cgacctgcgc
     2581 cgccgcctcg tccaccacac gatcgccttc aaccacgccc tgcgccagcg cctgcgcgac
     2641 gaggacgacc tcgacgagta cgaggtcatg ctcgacccgg agctgcgcgc cagcatcgtc
     2701 gccgacgtca accgcccgct ggcgatcacc tcgcacatgg ccggcctgct gcgcggggcc
     2761 tacgacgcgg gcttgatcca cccccagcac gtacctgtcc tcgaggccag cctcggcgtg
     2821 atgaccgacc tgcagggcgg ctgcgagcgg atccgcagca cgcccctgcc ctacgtctac
     2881 cgcgtcctcg tgcaccagat cgtcggcttt tactgcttct cgctgccgtt cggattaatt
     2941 cataccctgc agtggttcgc gccggtggtc gcggtgctcg tcgcgtatgc attcttcggg
     3001 ctcgacgcga tcggcgagga gctcgataac ccgttcgaga ccgacgccaa cgacctcccg
     3061 ctcgccgcga tcacgcgggg tattgagatt gacttgcgcg cgcggctcgg tgagaccgac
     3121 ctcccggcgg ccgcggtgcc gcatcacggg ctgctgctgt agttggcggg gcccggacag
     3181 ggcgtattcg ggagaagtgg gcggcgtggg cggtcggaca gggcgtgagc gaagaacgcg
     3241 gggcgatgct atctgggccg ggggggccga cggacggcgg gtccctgcga cgcgggtcct
     3301 cccaggggac gtgctcgccg ccccatctg
//
LOCUS       DASWNJ010001313         2504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_93788, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001313 DASWNJ010000000
VERSION     DASWNJ010001313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93788"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1635
                     /locus_tag="VGB85_21940"
     CDS             <1..1635
                     /locus_tag="VGB85_21940"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0136766.1"
                     /translation="YRELVQNSIDAGSAAVEVWMEYEPGAEDEGTIAIHVDDFGEGMN
                     EEIIDGQLTKLFSSSKDGDLTKIGKFGIGFVSIFAPDPRAVLLHTGRGGEAWEVMFHP
                     DRSFTKTRLETPVEGTQITLYIAGDRGRYRELVTGSRETLRRWCSHSDVDITFEDRSG
                     GAGPEAINLPFEVPGECLTRSAAEGTEVVLAFHGEPEYGFYNKGLALTVSRDGASLLG
                     EHAQRLAHVGFKVKSRYLEHTLSRDTVVREGNFLKAIELVLAAADGPLQTALIEQIAA
                     IGQQRAWSIAELRRYGELLGYLAREPDTALARHEERPLLRVVDGSAVSLAQLWEAASE
                     DRRVFVADALGPLAEQLIAAGTPVLLAARAPGERSPVAGVAGLVERYVRHRHRRTLRG
                     RLARLGLPFSTPELLVCAPEAAFVRVEMSTPRPEEARLIDAAAKLLAAVDAGYRRLVA
                     CTLPEDGPLFVLGRTISPMMQRPPDGRLFLDRRPRRPEAAVHVGHPLFRRWIDLQKTA
                     PALAAYCLAKDLLLIEDRLLGSDIALMAAARPEVAA"
     gene            1632..>2504
                     /locus_tag="VGB85_21945"
     CDS             1632..>2504
                     /locus_tag="VGB85_21945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136767.1"
                     /translation="MSTIDAVIAQARRPGNFSERRRFTLARTQAIQKLRQFALADPAA
                     YILELIQSAVANGATWIEVVRDSETVTVAYIGGGIPESALGRLFDFLFASKDDPGLGY
                     LRELAIGVNALMVFEPEKIIIESGDGTLAGTSRMELLAGEDRLDVGRPDHALSGTFIR
                     AEGLKRSRNVSRERSIIEMRCLAAPVPILYNSEVMFGHSRQRAPMLPGAFKRISFDEG
                     DLYGTIGLRDPGSPRNDFALLTRGVLIENVAHTLVAGSAFGGIICFDGLRKTADHARI
                     VRDDRMAEMWLRVLP"
BASE COUNT          353 a          845 c          945 g          361 t
ORIGIN      
        1 tatcgtgagc tggtccaaaa cagcatcgac gccggctcgg cggcggtcga ggtgtggatg
       61 gagtacgagc ccggcgccga ggacgagggc acgatcgcga tccacgtcga cgacttcggc
      121 gagggcatga acgaggagat catcgacggc cagctgacga agctgttctc gtcgagcaag
      181 gacggcgacc tgacgaagat cgggaagttc ggcatcggct tcgtctcgat cttcgcgccc
      241 gacccgcggg cggtgctgct gcacaccggc cgcggcggcg aggcgtggga ggtgatgttc
      301 caccccgacc gcagcttcac caagacgagg ctggagacgc cggtcgaggg cacgcagatc
      361 acgctgtaca tcgccggcga ccgcggccgc taccgcgagc tggtcaccgg cagtcgcgag
      421 accctgcgcc gctggtgctc acactcggac gtcgacatta ctttcgagga ccgcagcggc
      481 ggcgccggac cggaggcgat caacctgccc ttcgaggtgc ccggcgagtg cctgacccgc
      541 agcgcggccg agggcaccga ggtggtgctc gcgttccacg gcgagcccga gtacgggttt
      601 tataataaag ggttggcgct gacggtctcg cgcgacgggg cgtcgctgct cggcgagcac
      661 gcgcagcggc tcgcgcacgt cggcttcaag gtcaagtcgc gctatctcga gcacacgctg
      721 agccgcgaca cggtggtccg cgagggcaat tttttgaagg cgatcgagct ggtgctcgcg
      781 gcggcggacg ggccgctgca gacggcgctg atcgagcaga tcgcggcgat cgggcagcag
      841 cgggcgtggt cgatcgcgga gctgcggcgc tatggcgagc tgctcgggta cctcgcgcgc
      901 gagcccgaca cggcgctcgc ccgccacgag gagcgcccgc tgctgcgcgt ggtcgacggc
      961 agcgcggtca gcctggccca gctgtgggag gcggcgagcg aggaccggcg ggtgttcgtc
     1021 gccgacgcgc tcgggccgct ggcggagcag ctgatcgccg cgggcacgcc ggtgctgctg
     1081 gccgcgcgtg cgccgggtga gcgctcgccg gtcgcggggg tcgcggggct ggtcgagcgc
     1141 tacgtccggc atcgccatcg ccggaccctg cgcggacgtc tggcgcggct cggcctgccg
     1201 ttctcgaccc cggagctgct ggtctgcgcg cccgaggcgg cgttcgtgcg cgtcgagatg
     1261 agcacgccgc ggccggagga ggcgcggctg atcgacgcgg cggcgaagct gctggcggcg
     1321 gtcgacgccg gctatcgccg gctggtcgcg tgcaccttgc cggaggacgg gccgctgttc
     1381 gtgctcggcc ggacgatctc gccgatgatg cagcggccgc cggacgggcg gctgttcctc
     1441 gacaggcggc cgcgccggcc cgaggcggcg gtgcacgtcg ggcacccgct gtttcgccgc
     1501 tggatcgacc tgcaaaagac ggcgccggcg ctggcggcgt attgcctcgc caaggacctg
     1561 ctgctgatcg aggaccgcct gctcgggtcg gacatcgcgc tgatggccgc ggcgcgcccg
     1621 gaggtggcgg cgtgagcacg atcgacgcgg tcatcgccca ggctcgccgg cccggcaact
     1681 tcagcgagcg ccggcgcttc acgctcgcgc gcacgcaggc gatccagaag ctgcggcagt
     1741 tcgcgctcgc cgaccccgcg gcgtacatcc tcgagctgat ccagtcggcg gtcgccaacg
     1801 gggcgacgtg gatcgaggtc gtgcgcgaca gcgagacggt gacggtcgcg tacatcggcg
     1861 gcgggatccc ggagtcggcg ctcgggcggc tgttcgactt cctgttcgcc tcgaaggacg
     1921 acccggggct cggttatctg cgcgagctgg cgatcggcgt caatgcactg atggtcttcg
     1981 agccggagaa gatcatcatc gagtccggcg acggcaccct cgcgggtacg tcgcgcatgg
     2041 agctgctggc cggcgaggac cgcctcgacg tcggccggcc ggaccacgcg ctctcgggca
     2101 ccttcatccg cgccgagggg ctcaagcgca gccgcaacgt cagccgcgag cgcagcatca
     2161 tcgagatgcg ctgcctcgcc gcgccggtgc cgatcctcta caacagcgag gtgatgttcg
     2221 ggcactcgcg ccagcgggct ccgatgctgc ccggcgcgtt caagcggatc agcttcgacg
     2281 agggcgacct gtacggcacg atcggcctgc gcgacccggg cagcccgcgc aacgacttcg
     2341 cgctgctgac ccgcggcgtg ttgatcgaga acgtcgcaca tacactcgtc gcggggtcgg
     2401 cgttcggcgg gatcatctgc ttcgacgggc tgcgcaagac ggccgaccac gcccgcatcg
     2461 tccgcgacga ccgcatggcg gagatgtggc tgcgcgtgct gccc
//
LOCUS       DASWNJ010001314         2552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_306883, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001314 DASWNJ010000000
VERSION     DASWNJ010001314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306883"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VGB85_21950"
     CDS             <1..826
                     /locus_tag="VGB85_21950"
                     /inference="COORDINATES: protein motif:HMM:NF043005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SCE4755 family polysaccharide monooxygenase-like
                     protein"
                     /protein_id="HEY0136768.1"
                     /translation="ASLVLLAGPARAHISLSSPPARTEFQKDGPCGAGADQRGPTLAT
                     FKPGEKIVVTWTETVNHPGHFRIALDPDGQDGFFEPKAFDDVSGGPGVIVDGIADKDG
                     GEYSQEITLPNIECDSCVLQLIQVMTDKKPYGDGNDLYYQCADIAIVGEMVGTTGEPG
                     STGPDTTTSGASDPSAGETGTTGEPGSTGGGETAAGNTGGDEPTTGPATGDASTGGGT
                     GGGSTGDASSGGDSSGTTDGASEGSGCGCRGDAGGPRGAGWLALGLLSVLVRRRHA"
     gene            897..1496
                     /locus_tag="VGB85_21955"
     CDS             897..1496
                     /locus_tag="VGB85_21955"
                     /inference="COORDINATES: protein motif:HMM:NF043005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCE4755 family polysaccharide monooxygenase-like
                     protein"
                     /protein_id="HEY0136769.1"
                     /translation="MVGSIVLLIAGSAAAHIHVTSPVSRGTQQKEGPCGGGAGDTRGE
                     VVHSFRPGQTIVVEWDETVDHPGHFRISFDADGQDDFVDPASFTDLDGNAAILIDGIA
                     DGHGGSYRQEVTLPAVECDRCTLQVVQVMTDKPPYGDGNDLYYQCVDLVLSEDAEAEA
                     TTSSLDGASGGCDVGGGGPGAVMLLCGLLAIRRIRRACR"
     gene            1579..>2552
                     /locus_tag="VGB85_21960"
     CDS             1579..>2552
                     /locus_tag="VGB85_21960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0136770.1"
                     /translation="MAATQEFKDVVRAVFNQLDLMAWAIDTGGQILFSEGAALKNIGV
                     EPGGTVGYNIFELYGSSENVGDIRRGLAGHSHSALTRIDGRTLETRYEPMRDEADAIT
                     GMVGITRDITEQVNARDTLDRQTLAMREQAELLDLAHDAIRVRRLDGTITYWNQGAER
                     VFGHKRDDAIGKHVHALLRTEPVTGDLAAVERQLLTNGFWEGELTQRRSDGQEVLVAS
                     RWVLKRGATGERDAVLEIDTDITARRRAEEAEARRQQEIIRAQAQAIEELSTPLIPIA
                     DEILVMPLIGMMDAIRAQQVMHSLLTGLAASRGKFAILDITGVPVVDTA"
BASE COUNT          416 a          823 c          963 g          350 t
ORIGIN      
        1 cgcctcactc gtcttgctcg ccggtccagc gcgggcgcac atctcgctga gctcgccgcc
       61 ggcgcggacc gagttccaga aggatgggcc gtgcggggcg ggggcggacc agcgcgggcc
      121 cacgctggcg accttcaagc cgggcgagaa gatcgtcgtc acgtggaccg agacggtcaa
      181 ccatcccggc cacttccgca tcgcgctcga cccggacggt caggacggct tcttcgagcc
      241 gaaggcattc gatgatgtct cgggcgggcc gggcgtgatc gtcgacggca tcgccgacaa
      301 ggacggtggc gagtactcgc aggagatcac gctgccgaac atcgagtgtg acagctgcgt
      361 gctgcagctg atccaggtga tgaccgacaa gaagccgtac ggcgacggca acgacctgta
      421 ctaccagtgc gccgacattg cgatcgtcgg tgagatggtc ggcaccaccg gggagccggg
      481 atcgacgggc cccgacacga cgaccagcgg cgcgagcgac ccgagcgcgg gtgagaccgg
      541 cacgaccggc gagccgggca gcacgggtgg cggcgagact gcggcgggca acaccggcgg
      601 cgacgagccg acgacggggc cggcgaccgg cgacgcctcg accggcggcg gcacgggcgg
      661 tggttcgacc ggcgatgcga gcagcggtgg tgacagcagc ggcacgaccg acggcgcgag
      721 cgagggctcg ggttgcgggt gtcgcggcga tgccgggggt ccgcgcgggg cggggtggct
      781 ggcgctgggt ctgctgtccg tgctcgtcag gaggcgtcat gcctagtcga gcagcccggg
      841 atctgccggg cacagggcgc tctggacatg tccccggggc caggtggttg gtcggcttgg
      901 tcggatcgat cgtgctgctg atcgccggta gcgcggcggc gcacattcac gtgacctcgc
      961 cggtctcgcg cggaacgcag cagaaggaag ggccgtgcgg gggcggggcg ggcgacacgc
     1021 gcggcgaggt cgtgcacagc tttcgtccgg gccagacgat cgtggtcgag tgggacgaga
     1081 cggtcgacca ccccgggcat tttcgcatca gcttcgacgc cgacggtcag gacgacttcg
     1141 tcgatccggc gagcttcacc gacctcgacg gcaacgcggc gatcctgatc gacggcatcg
     1201 ccgacggcca tggcggcagc tatcgccagg aggtcacgtt gccggcggtc gagtgcgatc
     1261 gctgcacctt gcaggtggtt caggtgatga ccgacaagcc gccgtacggg gatggcaacg
     1321 acctctacta tcaatgcgtc gacctcgtgt tgagcgagga cgcggaggcc gaggcgacga
     1381 cgtcgtcgct cgatggtgcg agtggcgggt gcgatgtagg cggcggcgga ccgggcgcag
     1441 tgatgctgct gtgcgggctg ctggcgatcc gtcgaatacg tcgcgcctgt cgttgacgca
     1501 tccacaatcg cggtcgcgag cgtcgacgag atcgtcgcgg ctcgccgtcg cggtgcgact
     1561 cgctggtaga ctgcctcgat ggctgcgacg caggaattca aggatgtcgt ccgggcggtg
     1621 ttcaaccagc tcgacctgat ggcctgggcg atcgacaccg gcggccagat cctgttctcc
     1681 gagggcgcgg cgctgaagaa tatcggcgtc gagccgggcg ggacggtcgg ctacaacatc
     1741 ttcgagctct acggatcctc cgaaaacgtg ggcgacatcc gccgcgggct cgctggccac
     1801 agccacagcg cgctgacccg gatcgacggc cgcacgctcg agacccgcta cgagccgatg
     1861 cgcgacgagg cggacgcgat caccgggatg gtggggatca cgagggacat caccgagcag
     1921 gtgaacgccc gcgacacgct cgaccgccag accctggcga tgcgcgagca ggccgagctc
     1981 ctcgacctcg cccacgacgc gatccgcgtg cgtcggctcg atggcacgat cacatactgg
     2041 aatcagggcg ccgagcgggt gttcggccac aagcgcgacg acgcgatcgg gaagcacgtg
     2101 cacgcgctgc tgcgcacgga gccggtgacc ggcgatctcg cggcggtcga gcgccagctg
     2161 ctcaccaacg gcttctggga gggcgagctg acccagcggc gcagcgacgg ccaggaggtg
     2221 ctggtcgcga gtcgctgggt gctcaagcgc ggcgcgacgg gcgagcgcga cgcggtgctc
     2281 gagatcgaca cggacatcac ggcccgccgg cgcgccgagg aggcggaggc ccgtcgccag
     2341 caggagatca tccgcgcgca ggcccaggcg atcgaggagc tgtcgacccc gctgatcccg
     2401 atcgccgacg agatcctcgt catgccgctg atcggcatga tggacgcgat ccgcgcgcag
     2461 caggtgatgc acagcctgct gaccgggctg gccgcgtcgc gcggcaagtt tgcgatcctc
     2521 gacatcaccg gcgtgccggt cgtcgacacc gc
//
LOCUS       DASWNJ010001315         2744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_588737, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001315 DASWNJ010000000
VERSION     DASWNJ010001315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588737"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VGB85_21965"
     CDS             <1..883
                     /locus_tag="VGB85_21965"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEY0136771.1"
                     /translation="QGQIDKETRERWTAGLRAGLARGVAPLVEPGEMPEVDASCDRKG
                     CYDKVRSATGATHVVRTTLVAKNRDFLLKLDLVDAETGEVTLSQDEVCEICGSEEVVG
                     LLDSQGALLQTRLDALGTGPAVLILDSKPRGAMVTIDGEVVGTTPLERPVLEGVHTVR
                     LSLDGHVAEERELTLVNGAREQLSLTLKRTPGNAKSRALGAAGLGGGLALLGAGIALL
                     VLDDKPYKANCSGENVDKDMDCRFLFNTAPLGGALIGVGAVLATLGAVALYRNRGAKA
                     PRRAMLRPGGLGVVGRF"
     gene            1003..>2744
                     /locus_tag="VGB85_21970"
     CDS             1003..>2744
                     /locus_tag="VGB85_21970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HEY0136772.1"
                     /translation="MGVLSTIWALIGNRPVFTYEVEGLAEPPRVTRLSGHEGLSRPYE
                     FRVELACGPEVELADLSGKAGALCIEGIDEPRFVHGIIGEVDYVGESRNYGLFEVVLV
                     PTIWRLKHRHDCRIFQHKTTEEVVRQVLKAAGVSAKAYRFDLHGNYTARNYCVQYRES
                     DLAFISRLLEEDGIFYFFEHTAESHVMVIADHVGAHPRIPGADTVWWNPPGGLLHARE
                     HVLDFRFGQRIRPGTATLRDFHFPTPTADMQAREQAADDRDLEVYDYPGEYREAAGAS
                     LDRGQMLAKVRLQELQSSRRVGSGKADCMRLVPGHTFHLAGHGRGELNLEYLLTGVVH
                     HGRQPQALDEDAAGEFSYSAEFTCMERSVPYRPARATPRPFVRGLQSATVVGPAGEEV
                     HTDDQGRVMVQFHWDRQGERDEQSSCWVRVSQLWAGAGWGAMFLPRIGHEVLVDFLEG
                     DPDRPIITGRAYHGLNDLPYALPAEKTKSTIKSDSSRGGGGYNELRFEDRKGEEQVFI
                     RAEKNLDIFVQHDRMEDVHHDRHLRVGETTPQGKVGDYNQLVLRDKHTRVERDTHTHI
                     GGNVLLRIGGIDGEG"
BASE COUNT          415 a          937 c         1015 g          377 t
ORIGIN      
        1 ccagggccag atcgacaagg agacccgcga gcgctggacg gcggggctgc gcgccggcct
       61 cgcgcgcggg gtcgcgccgc tcgtcgagcc gggtgagatg cccgaggtcg acgcgagctg
      121 cgaccgcaag ggctgctacg acaaggtccg cagcgcgacc ggggcgaccc acgtggtccg
      181 cacgacgctg gtcgccaaga accgcgactt cctgctgaag ctcgacctcg tcgacgccga
      241 gaccggcgag gtgacgctca gccaggacga ggtgtgcgag atctgcggat ccgaggaggt
      301 ggtcggcctg ctcgactcgc agggcgcgct gctgcagacg cgactcgacg cgctcggcac
      361 cggccccgcg gtgctgatcc tcgacagcaa accccgcggc gcgatggtga cgatcgacgg
      421 cgaggtcgtc ggcacgacgc cgctcgagcg gccggtgctg gaaggcgtgc acacggtccg
      481 cctctcgctc gacggacacg tcgcggagga gcgcgagctg acgctcgtca acggggcgcg
      541 cgagcagctg tcgctgaccc tcaagcgcac gcccggcaac gccaagagcc gcgcgctcgg
      601 tgcggccggg ctcggcggcg gtctggcgct gctcggggcc ggcatcgcgt tgctggtgct
      661 cgacgacaag ccctacaagg cgaactgctc cggcgagaac gtcgacaagg acatggactg
      721 ccggttcctc ttcaatacgg cgccgctcgg cggggcgctg atcggggtgg gggccgtgct
      781 ggcgacgctc ggggcggtcg cgctctatcg caatcgcggg gcgaaagctc cgcggcgggc
      841 gatgctgcgg ccgggcgggc tcggcgtggt cgggcgcttc tagcgggacg gagcgcgcat
      901 cggcacgcgc gcatcgacag ggtcgcgcgt cgccgccagt cgggcgcgct tgctggcctc
      961 gcgggcatcc tgttagcgtg cggccgtggg ggcgggaggg tcatgggcgt actgagcacg
     1021 atctgggcgt tgatcggcaa tcggccggtg ttcacgtacg aggtggaggg cctcgcggag
     1081 cccccgcgcg tgacgcggtt gagcggccac gagggcctgt cgcgcccgta cgagtttcgc
     1141 gtcgagctcg cgtgcgggcc ggaggtcgag ctcgccgacc tcagcggcaa ggccggggcg
     1201 ctgtgcatcg agggcatcga cgagccccgg ttcgtgcacg gcatcatcgg cgaggtcgac
     1261 tacgtcggcg agagccgcaa ctacgggctg ttcgaggtgg tgctggtgcc gacgatctgg
     1321 cgcctgaagc accgccacga ctgccggatc tttcagcaca agaccaccga ggaggtggtg
     1381 cgtcaggtgc tcaaggccgc cggcgtctcg gcgaaggcct accgcttcga tctccacggc
     1441 aactacaccg cgcgcaacta ctgtgtgcag tatcgcgagt cggacctggc gttcatctcg
     1501 cgcttgctcg aggaggacgg gatcttctac ttcttcgagc acaccgcgga gtcacacgtg
     1561 atggtgatcg ccgaccacgt cggcgcccat ccgcggatcc ccggcgccga cacggtgtgg
     1621 tggaaccccc cggggggcct cctgcatgcc cgcgagcacg tcctcgactt tcgcttcggt
     1681 cagcggatcc gccccggcac ggcgacgttg cgcgacttcc acttcccgac cccgaccgcc
     1741 gacatgcagg cgcgcgagca ggccgcggac gaccgcgacc tcgaggtcta cgactatccc
     1801 ggcgagtacc gcgaggccgc cggcgcgagc ctcgatcgcg gtcagatgct cgccaaggtc
     1861 cgcctgcagg agctgcagag ctcgcggcgc gtcggctccg gcaaggccga ctgcatgcgc
     1921 ctggtccccg gccacacctt tcacctcgcg ggccacgggc gcggcgagct caacctcgag
     1981 tacctgctga ccggcgtggt ccaccacggc cgccagccgc aggcgctcga cgaggacgcg
     2041 gccggcgagt tctcgtacag cgcggagttc acgtgcatgg agcggtcggt gccgtatcgt
     2101 ccggcgcgcg cgaccccgcg gccgttcgtg cgcgggctgc agtcggcgac cgtggtcggc
     2161 ccggccggcg aggaggtgca cacggacgac caggggcggg tgatggtgca gtttcactgg
     2221 gaccgccagg gcgagcgcga cgagcagagc tcgtgctggg tgcgggtcag tcagctgtgg
     2281 gcgggcgccg gctggggcgc gatgttcctg ccgcggatcg gccacgaggt gctcgtcgac
     2341 ttcctcgagg gcgaccccga ccggccgatc atcaccggtc gcgcctatca cggcctcaac
     2401 gacctgccct acgcgctgcc cgccgagaag accaagagca cgatcaagtc ggacagctcg
     2461 cgcggcggcg gcggctacaa cgagctgcgc ttcgaggacc gcaagggcga ggagcaggtg
     2521 ttcatccgcg ccgagaagaa cctcgacatc ttcgtccagc acgaccgcat ggaggacgtg
     2581 caccacgacc gccacctgcg ggtcggcgag accacgccgc agggcaaggt cggcgactac
     2641 aaccagctgg tgctgcgtga caagcacacg cgggtcgagc gcgacacgca cacgcacatc
     2701 ggcggcaacg tccttttgcg gatcggcggc atcgacggcg aggg
//
LOCUS       DASWNJ010001316         4180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_59429, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001316 DASWNJ010000000
VERSION     DASWNJ010001316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59429"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2099
                     /locus_tag="VGB85_21975"
     CDS             <1..2099
                     /locus_tag="VGB85_21975"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0136773.1"
                     /translation="EDLLRPRSPTGERQPRSLAPAHAVRIALAVCEGLIAAHAAGVVH
                     RDLKPANILIEPGGRVVITDFGIARGLADDGGRTQGMVGTPLYMAPEQVEGAPVDGRT
                     DLYALGLTLFEMLTGAQPFIADAPLIAALRRIEEPPPDPRTRCPELPAALAQIVLDCL
                     AREPHGRPASAAALADRLRGWLATQADVPELASPIALPAPPVDARPITAQASRGASIP
                     ARGGSGSLRPGATSECALAVLPVRYQGPADERDLADAVTDALIDVLSRTGGLRVASSG
                     ATARFRDNRDPRAVGADLGVDVIVDATLQRGPSQVRLLARLVEVTTGVQLWSDRFELR
                     PEDPFEIADHVSKRLAESLRVELMTLVHRADADADSIALYTRARRKLYAMHVLGFDSA
                     VELLEGSLAIAPEFRPALAAHAVACVRAWFIARSQSGEATPRDLEGDAQRAVERALLV
                     APAIAETHLARGMLAVQRGEWREAVKALVKALELAPAYPHALQYLGQLQCEAGNTREG
                     VARARLAADLEPSLALGLLEAARVHALHGELDEYERLMARIEDHPPYRFSILLLRMRV
                     AAWFGDRELPRQILAAQHGDILAGYNRILIGYGRALLGDVDRAEMDAFIRDLLTSNLS
                     PRLYTAICQLCVELHCARGDLEAALAHFRLAADAVLTDIEWVDRCPLLAPMRALPGFG
                     AVRRTVRLRVDQMWML"
     gene            2146..3663
                     /locus_tag="VGB85_21980"
     CDS             2146..3663
                     /locus_tag="VGB85_21980"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEY0136774.1"
                     /translation="MAKSAPLVHESDWKSRAVWSNDGYFSLETGDTGDVPVRMFVTQK
                     LLTEAEPTLYRQLINATRFPGVKLVAVTPDVHYGYGVPVGCVILTDRAEGSVAMGPVG
                     FDIGCGMMSARSDVAAELATPDRKLEFNREVIRRVSLGAGGKSVKLGSLSQTEFQDLI
                     RGGAEHYVETYGAAFDRSRAERHRIPVEDSWNPPWGGKGSPERGLDQLGSLGGGNHFI
                     ELQRCEETGTLFVQVHTGSRGFGHGLATNYFEMAKAEHAGLGGEVSDIDLGYFTPQSR
                     HFRSYLNAVAAGGNFAILNRLVIFEQIAEAFRKVFKADLELIYEISHNLVQAEDHPEF
                     GEVWVHRKGATRAFPGGHAALKGTIWQDTGHPVLIPGSNRDYSYILMPEPGAAKSGFS
                     VNHGAGRRMSRGEAMRQLDQQKVNEQYRRDGILVNLNGDVPLDESNQCYKSSEEVISA
                     VVAAGLARVQYTLWPLASIKGDEKGASRAARKAKKNQERSRIKDRDAARRTKEHF"
     gene            complement(3691..>4180)
                     /locus_tag="VGB85_21985"
     CDS             complement(3691..>4180)
                     /locus_tag="VGB85_21985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136775.1"
                     /translation="GPGGLGVLPGVALGYRRELPRVVGVQADLITMMTPGWRSQPSGS
                     IVIGLVGARALLVVQARRDRRVVPRIGLGGGGAVVWAIGRAPQPLRGNPDVGVVPLLS
                     ASVALAVRVRPRLGVVLGGGLDVLLSPVVVRVAGDERAQLGPAMLHGSLGLEWSWPVR
                     SK"
BASE COUNT          612 a         1682 c         1355 g          531 t
ORIGIN      
        1 tcgaggacct gctgcgcccg cgcagcccca ccggcgagcg ccagccccgt tcgctcgcgc
       61 cggcgcacgc ggtccgcatc gcgctcgccg tgtgcgaggg cctgatcgcc gcgcacgcgg
      121 ccggcgtggt ccatcgcgac ctcaagccgg ccaacatcct gatcgagccc ggcggtcgcg
      181 tcgtcatcac cgacttcggc attgcccgcg gcctcgccga cgacggcggc cgcacccagg
      241 gcatggtcgg cacgccgctc tacatggcgc ccgagcaggt cgagggcgcg ccggtcgacg
      301 gccgcaccga cctttatgcg ctcggtctga cgctgttcga gatgctgacc ggcgcccagc
      361 cgttcatcgc cgacgccccc ctgatcgccg cgctgcgccg catcgaggag ccgccgcccg
      421 acccgcgcac gcgctgcccg gagctgcccg ccgcgctcgc gcagatcgtc ctcgactgcc
      481 tcgcccgcga gccccacggc cgccccgcca gcgccgccgc cctcgccgac cgcctgcgcg
      541 gatggctggc gacgcaggcc gacgtgccgg agctcgccag cccgatcgcc ctgcccgcgc
      601 ctcccgtcga cgcccgaccg atcaccgcac aggcatcgcg cggcgcctct atcccggcac
      661 gcggcggcag cggctcgctg cgccccggcg cgacctccga gtgcgccctc gccgtgctgc
      721 ccgtgcgcta ccagggcccc gccgacgagc gcgacctcgc cgacgccgtc accgatgcgc
      781 tgatcgacgt gctgtcgcgg accggcggcc tgcgcgtcgc cagcagcggc gcgacggccc
      841 gcttccgcga caaccgcgac ccccgggccg tcggcgccga cctcggcgtc gacgtcatcg
      901 tcgacgcgac gctgcagcgc ggcccctcgc aggtgcggct gctcgcccgc ctcgtcgagg
      961 tcaccaccgg cgtgcagctg tggagtgacc gcttcgagct gcgccccgag gacccgttcg
     1021 agatcgccga ccacgtcagc aagcgcctcg ccgagagcct gcgcgtcgag ctgatgaccc
     1081 tcgtccaccg cgccgacgcc gacgccgatt cgatcgccct ctacacccgc gcccgccgca
     1141 agctctacgc gatgcacgtg ctcggcttcg acagcgcggt cgagctgctc gagggcagcc
     1201 tggcgatcgc gccggagttt cgccccgcgc tcgccgccca cgcggtcgcc tgcgtgcgcg
     1261 cgtggttcat cgcgaggtcg cagagcggcg aggccacccc gcgcgacctc gagggcgacg
     1321 cgcagcgagc cgtcgagcgc gccctgctcg tcgccccggc gatcgccgag actcacctcg
     1381 cgcgcggcat gctcgcggtc cagcgcggcg agtggcgcga ggccgtcaaa gcgctcgtca
     1441 aggcgctcga gctggcgccc gcctacccgc acgcgctgca gtacctcgga cagctgcaat
     1501 gcgaggccgg caatacccgc gagggcgtcg cccgggcccg gctcgccgcg gacctcgagc
     1561 cgagcctcgc gctcggcctg ctcgaggccg cccgcgtgca cgccttgcat ggagagctcg
     1621 acgagtacga gcgcctgatg gcccgcatcg aggaccaccc gccctaccgc ttctcgatcc
     1681 tcctgctgcg catgcgcgtc gcggcatggt tcggcgaccg cgagctcccg cgccagatcc
     1741 tcgccgcgca gcacggcgac atcctcgccg gctacaaccg gatcctgatc ggctacggcc
     1801 gggccctgct cggcgacgtc gaccgcgccg agatggacgc gttcatccgc gacctgctga
     1861 ccagcaacct gagcccgcgc ctctacaccg cgatctgcca gctgtgcgtc gagctccatt
     1921 gcgcccgcgg cgacctcgag gccgcgctcg cgcactttcg cctcgccgcc gacgccgtgc
     1981 tgaccgacat cgagtgggtc gaccgctgcc cgctgctcgc cccgatgcgc gctctccccg
     2041 gcttcggcgc cgtgcgccgc acggtccgcc tgcgggtcga ccagatgtgg atgctctgag
     2101 gtccgccggg cccggccccg actccgcgct atactccggg ccaccatggc caagtctgcc
     2161 cccctcgttc acgagagtga ctggaaatcg cgggccgtct ggtccaacga cggctacttc
     2221 agcctcgaga ccggcgacac cggcgacgtg cccgtgcgca tgttcgtcac gcagaagctg
     2281 ctgaccgagg ccgagccgac gctctatcgc cagctgatca acgcgacgcg cttcccgggc
     2341 gtcaagctcg tcgccgtcac ccccgacgtg cactacggct acggcgtgcc ggtcggctgc
     2401 gtcatcctca ccgaccgcgc cgagggctcc gtcgccatgg gtccggtcgg cttcgacatc
     2461 ggctgcggca tgatgagcgc gcgcagcgac gtcgccgccg agctcgccac ccccgaccgc
     2521 aagctcgagt tcaaccgcga ggtcatccgc cgcgtcagcc tcggcgccgg cggcaagagc
     2581 gtcaagctcg gcagcctgtc gcagacggag ttccaggacc tgatccgcgg cggcgccgag
     2641 cactacgtcg agacctacgg cgccgccttc gaccgcagcc gcgccgaacg ccaccggatc
     2701 cccgtcgagg actcgtggaa ccccccgtgg ggcggcaagg gctcgcccga gcgcggcctc
     2761 gatcagctcg gctcgctcgg cggcggcaac cacttcatcg agctccagcg ctgcgaggag
     2821 accggcaccc tgttcgtgca ggtgcacacc ggatcccgcg gcttcggcca cggcctggcg
     2881 accaactact tcgagatggc caaggccgag cacgccggcc tcggcggcga ggtctcggac
     2941 atcgacctcg gctacttcac gccccagtca cggcactttc gcagctacct caacgccgtc
     3001 gccgccggcg gcaacttcgc gatcctcaat cgcctcgtca tcttcgagca gatcgcggag
     3061 gcgtttcgta aggtgttcaa ggccgacctc gagctcattt acgagatcag ccacaacctc
     3121 gtgcaggccg aggaccaccc ggagttcggc gaggtgtggg tgcaccgaaa aggcgcgacg
     3181 cgtgcgttcc ccggaggcca cgccgccctc aaaggaacga tctggcaaga cacggggcac
     3241 cccgtgttga tcccggggtc caaccgcgat tacagctaca tcctgatgcc cgagccgggc
     3301 gccgccaaga gcggcttctc cgtcaaccac ggcgccggac gccgaatgtc gcgcggcgag
     3361 gcgatgcggc agctcgacca gcaaaaggtc aacgagcagt accgccgcga cggcatcctc
     3421 gtgaacctca acggcgacgt cccgctcgat gaatccaacc agtgttacaa gagctccgag
     3481 gaggtcatct ccgcggtggt cgccgccggg ctcgcgcgcg tgcaatacac gctgtggccg
     3541 ctcgcctcga tcaagggcga cgagaagggc gcctcccggg ccgcccgcaa ggccaagaag
     3601 aaccaggaac gctcgcgcat caaggaccgc gacgccgccc gccgcaccaa agaacatttt
     3661 tgaaaggtcc gtcgctccgc ctcggcgcga ctacttggag cgcaccggcc acgaccactc
     3721 gaggccgagc gatccgtgca gcatcgcggg tccgagctgc gcacgctcgt cgccggcgac
     3781 ccggacgacg accggcgata gcaagacatc gagcccgccg ccgaggacca cacccagtcg
     3841 cgggcgcaca cgcacggcga gcgcgaccga ggccgacagc agcgggacga ccccgacgtc
     3901 gggattgcct cgcaggggct gcggcgcgcg gccgatcgcc cacacgaccg ccccgccccc
     3961 accgagcccg atccgcggga ccacccgccg gtctcgccgc gcctgcacga ccagtagggc
     4021 ccgcgcgccg accagcccga tcacgatcga cccgctgggc tgcgaccgcc agcccggggt
     4081 catcatggtg atcaggtcgg cctgcacgcc gacaaccctc ggcagctcgc ggcggtagcc
     4141 gagcgcgacc ccgggcagca cgccgagccc gcccggcccg
//
LOCUS       DASWNJ010001317         2266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_588775, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001317 DASWNJ010000000
VERSION     DASWNJ010001317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588775"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..1699
                     /locus_tag="VGB85_21990"
     CDS             74..1699
                     /locus_tag="VGB85_21990"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0136776.1"
                     /translation="MKMRRLVMSVASWAIACGGGAGADGTGQDTASEDVDTGASAPTG
                     GETSEPPTPTCESVGGSATVLAPELLIILKDRWEEAWLASPAIADLDGDGVPEIVVPR
                     GEAVLAWHADGTLLWKAGASGRVWASPVVADFTGDGKLEVAFASREQVHMLDAAGMPV
                     PGFPVSWQDELRSLAAGDLDGDGDLDLAAALANGGPEDVIHAWDGQGQPVAGFPPNAT
                     GSSGCDDKCYLAGCYDQNLAVGDLDGDGRADLVAPHDNAYASIHRGSGEAFDAAATYM
                     VKKTPGVRYLHSLAESMQGYADDEASALQAHFTNTAPAIADIDGDGRREVIMLASVQN
                     AAQDQREQGVALWVVGSDAARHPAFPEPLHFPGYRSGLWDYGDNIVAVTNSVSVADIS
                     AEPGLEIVFADFDGTIHAVTAAGATLWSYTFTADAQVVTAGVAIADLSGDGSPEIVFN
                     TYSTAQGRGELVILDAQGSPLHALPLPRRGAMPVPTIGDVDGDGVADIVVSLKDAEDK
                     LESVYVYRVPGSAMNCLPWPTGRGNSLRSGLVP"
     gene            1892..>2266
                     /locus_tag="VGB85_21995"
     CDS             1892..>2266
                     /locus_tag="VGB85_21995"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0136777.1"
                     /translation="MLPKLDMRSTRKPVLISVYGPTPGTVYPIDGELVIGRADDASIT
                     VQDDRVSRHHTRLVVRGPESVHVEDLGSKNGTWINGRRVATAELQNGDTLQVGNETVF
                     HFVYRDPIEQRLQERQKLEAIGR"
BASE COUNT          353 a          752 c          838 g          323 t
ORIGIN      
        1 actacgaggc cgagttcgac gtcgacgcgg tctgccgggc ctgacgtaca ctcgtggctc
       61 cccggaggga gcgatgaaga tgcgacgcct ggtgatgtcg gtggcctcgt gggcgatcgc
      121 ctgcggcggc ggtgcgggtg cggacgggac cggccaggac acggcgtccg aggacgtcga
      181 taccggagcg agcgcgccca ccggcggcga gaccagcgag ccgcccacgc cgacttgcga
      241 gtcggtcggc ggcagcgcga cagtgttggc gccggagctg ctgataattt taaaagatcg
      301 gtgggaggag gcgtggctgg cctcgccggc gatcgccgac ctcgatggtg atggtgtgcc
      361 ggagatcgtc gtgccccgcg gggaggcggt gctcgcgtgg cacgccgacg gcaccttgtt
      421 gtggaaggcc ggagcgagtg ggcgcgtgtg ggccagcccg gtggtcgcgg acttcaccgg
      481 cgacggcaag ctcgaggtcg cgttcgccag ccgtgaacag gtgcacatgc tcgacgccgc
      541 gggtatgccg gtacctggct ttccggtcag ctggcaggac gagctgcgca gcctcgcggc
      601 cggcgacctc gacggcgacg gcgacctcga cctcgcggcc gcgctggcga acggcggtcc
      661 agaggatgtg atccacgcgt gggacgggca gggccagccg gtcgcaggct tcccgccgaa
      721 cgcgaccggc agcagtggtt gcgacgacaa gtgttacctc gctggttgtt acgaccagaa
      781 cctcgcggtc ggcgacctcg acggtgacgg ccgggccgac ctggtcgcgc cgcacgacaa
      841 cgcctacgcg agcatccacc gcggcagcgg cgaggccttc gacgccgcgg cgacctacat
      901 ggtcaagaag accccgggcg ttcgctatct acactcgctg gccgagtcga tgcagggcta
      961 cgcggacgac gaggcgagcg ctctacaggc ccatttcacc aacaccgcgc cggcgatcgc
     1021 ggacatcgac ggggacggca ggcgcgaggt catcatgctc gcgagcgtac agaacgccgc
     1081 gcaggaccag cgcgagcagg gcgtcgcgct gtgggtcgtc ggctccgacg ccgcgcgcca
     1141 ccccgccttt cccgagccgc tgcactttcc cgggtaccgc tcgggcctgt gggattacgg
     1201 ggacaacatc gtcgcggtca ccaattcggt cagcgtcgcg gacatcagcg ccgagccggg
     1261 cctcgaaatt gtgttcgcgg acttcgacgg gacgatccac gcggtcacgg cggcgggcgc
     1321 gaccctgtgg agctacacct tcaccgccga cgcgcaggtc gtcaccgcgg gtgttgcgat
     1381 cgcggatctc tccggggatg gctcgccgga gatcgtcttc aacacctaca gtaccgcgca
     1441 ggggcgcggc gagctggtga tcctcgacgc gcaggggtca ccgctgcacg cgctgcccct
     1501 gccgcggcgc ggcgcgatgc cggtgccgac gatcggcgat gtcgacggcg atggcgtcgc
     1561 cgacatcgtc gtgtcgctca aggacgccga ggacaagctc gagagcgtgt atgtgtatcg
     1621 ggtgccgggc tcggcgatga actgcctgcc gtggccgacc ggtcgcggga attcgctgcg
     1681 cagcgggctc gtgccctgaa gcggccggcg gcggcgcggg cgacggcccg ggcgaatgtg
     1741 cgagatctgc gcagcaaccc cgaccgcgcc ctgcgtgtgc gcccgattgc agggtcgagg
     1801 gggagccctt gccagatccg cggggcggcg ttcaagatcg tcgtcggtgc cctttagccc
     1861 cgaccaggcg cgcgcgactc atcccgccgg gatgttgccg aagctcgaca tgcggagcac
     1921 ccgcaagccg gtgctgatct ccgtgtatgg cccgacgccg ggcacggtgt acccgatcga
     1981 cggcgagctg gtgatcggtc gcgctgacga tgcatcgatc accgtgcagg acgaccgcgt
     2041 ctcgcggcac cacacgcggc tggtcgtccg cggcccggag agcgtgcacg tcgaggacct
     2101 cggcagcaaa aacggcacgt ggatcaacgg gcgtcgggtg gccacggccg agctgcaaaa
     2161 cggcgacacg ctgcaggtcg gcaacgagac ggtgtttcac ttcgtctacc gcgacccgat
     2221 cgagcagcgc ctgcaagagc gccagaagct cgaggcgatc ggccgc
//
LOCUS       DASWNJ010001318         9995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_516250, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001318 DASWNJ010000000
VERSION     DASWNJ010001318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 9995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9995
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516250"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VGB85_22000"
     CDS             <1..355
                     /locus_tag="VGB85_22000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136778.1"
                     /translation="DLETFEKALVADPTLLAHAKFADIFASPADNVLVFFADLLGYQE
                     PYNVPGTVNDDNWSLRITPDYATAYAEHCRRRTALDLPTALAMALRRADQPPSPDLTE
                     VLAALDALGQASTAP"
     gene            395..1450
                     /locus_tag="VGB85_22005"
     CDS             395..1450
                     /locus_tag="VGB85_22005"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0136779.1"
                     /translation="MRLEHAQIRNFKAIRELSLSLLTTRGDPRPLTLLLGDNGSGKTT
                     ALQAIALTLSLATRRISAPAGLDWQGFLAERVGSLGPSRVALDVCFEDEELKTTRNLF
                     DTWIAQQPLELQHRFAAPGDMKRVELIYEKGALFSPQGPGAISQFLGRHFIRDLSKTN
                     EAARDSFSQIGDVFWFDQNRNPTTTGAELAWDMGINRLRSELIQWYAYHTSRRVIPAN
                     DYLQKLEDMFARFFPGARFVGIEPREVFALGDFESYFLIQRGDRVYDIAEMSSGEQAI
                     FPLLHEFARRSIARSVVLIDELELHLHPPLQQMLLSALRTIGPDCQFIVTTHSPYLEE
                     ATPDEFEIRLPGGRRCL"
     gene            1441..2265
                     /locus_tag="VGB85_22010"
     CDS             1441..2265
                     /locus_tag="VGB85_22010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136780.1"
                     /translation="MPLSLLYVEGSLDVILMAGVASTLSEGAPSVVSGGSKGALRPRV
                     MEARNKPQPIEACYLRDRDFDTDPPGTLNVPTADKNAQGDVIGWRWCRHEIENYLLEP
                     RIVAQATGWNEADFIRSLLAAARALRSYTAARWAVGTARRSLPPLYELCTRPESLKNE
                     IKVPGDLSEQACRRWITEYVGDFHSKVALQLADTALASTFEHWSRRLDRVSTPQEALL
                     DHSGKDLLAALEPEIAQRPLNNPIVFRNAIRDWIVANPALALAHLPEWAALLRGFA"
     gene            2587..4584
                     /locus_tag="VGB85_22015"
     CDS             2587..4584
                     /locus_tag="VGB85_22015"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HEY0136781.1"
                     /translation="MNQPIPTPPSPRAPAPIIVEGVMPELDAGRYAVKRVVGDELEVS
                     AAIFKDGHDLLAARVRYRGPGDTDWRMKRMRYDNDADRWSTHFPLDRVGTWTFVVEAW
                     TDAFATWRSELEKKHNAAQDITSELLEGAQLLEHTASRVEYPKAKAQMLRLAGLLRDT
                     DFEPYTRVRVGVAPELLAVMEAHYIPVDLSTFEREITVHVDRERARFAAWYEFFPRSA
                     TTDPKRHGTFADATKMLDRIAWLGFDVVYLPPIHPIGVANRKGPNNTLTPGPDDPGSP
                     WAIGNEHGGHTAIEPKLGTLEDFDRFVKRAGELGIEIALDYALQCSPDHPWLKEHPEW
                     FFVRPDGTIKYAENPPKKYQDIYPLNFWCKDREGLWNACLDIFLFWIGHGVRTFRVDN
                     PHTKALSFWEWVIAEVRKQHPDVIFLAEAFTRPNRMRYLAKAGFSQSYTYFTWRNSAF
                     ELQEYLTELTQGPMREYFRGNLFPNTPDILHEFLQHGGRGAFRARLLLAATLSPVYGI
                     YSGFELLENTPVRPGSEEYLDSEKYQIRVRNFDAPGNLNEDMRRLNQIRRENRALQLY
                     DNLRFHHSENGAVLFYRKSFFKNDLLIIVNCDPHNPQETMVHVPLAELGLNEHTPFVV
                     EDLLTGARYTWQGVRNYVRLDPKIQPGHLLRLVRPEGEQHT"
     gene            4587..6248
                     /gene="treS"
                     /locus_tag="VGB85_22020"
     CDS             4587..6248
                     /gene="treS"
                     /locus_tag="VGB85_22020"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEY0136782.1"
                     /translation="MDPLWYKDAIIYQIHLKSYYDSNRDGFGDFAGLIQKLDYIQSLG
                     VNTLWLLPFYPSPLRDDGYDIAEYKGINPQYGTLDDFSRFMEEAHRRHIRVITELVVN
                     HTSDQHPWFQRARNAPAGSPERDFYVWSDTAEKYTDARIIFTDTEKSNWTWDPVARQF
                     YWHRFFSHQPDLNFDNPVVTEAILDVMRFWFAMGVDGMRLDAVPYLIEREGTNCENLP
                     GTHAILKQIRAALDSEFEGRLLLAEANQWPPDVVPYFGDGTDECHMAFHFPLMPRMYI
                     AQRREDATPIIEIMERTPEIPPQCQWAIFLRNHDELTLEMVTDEERDYMYREYARDPR
                     MRINVGIRRRLAPLMDNGRRQIELMNALLMSMPGTPIVYYGDEIGMGDNVYLGDRNGV
                     RTPMQWTGDRNAGFSQGDTAALYSPLIVDAPYSYHAINVEAQEKFPSSLLHWMRSLIR
                     VRKQYKAFGRGTWESLPSANRRVLVFLRKYENETLLCVNNLSRYVQYTEVDLARFDGC
                     SPIELFSEGPFPPITDKPYLLTLGPYSFLWFRLLKTEKAQEMAIP"
     gene            6245..7702
                     /locus_tag="VGB85_22025"
     CDS             6245..7702
                     /locus_tag="VGB85_22025"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY0136783.1"
                     /translation="MTTTPAHDLPTLEISDNLGRAALGALGRDQLAAYLSARRWFGAK
                     GQVIEAAGFDEVAPLAAGDDAIALARIAVRFASGQTEHYQIPLAIDRSGSVSSLVLAN
                     LHGGGVLYDAVESPEFRRQLGRAFAHKATAVAQTVRWEFEPLTDLADMADAETRIVGG
                     EQSNTSIVFGTRAIMKLFRHVETGENPDVEIGRFLTTRTSFRGTPALLGVLHMRGPDG
                     DGVAGMVQEFLPGSSDGWAHVLAHLGKHGADESLITQLQALGRLTRELHEALASVSDD
                     PDFSPEPTEQAHLDRWRTDIDTQVSTTLTLLGTQTNKLPPQIATAATALIARGDELRA
                     LLKSSTGASAAGPRIRHHGDYHLGQVLHTADGGWRIIDFEGEPARPISERRAKHHPLR
                     DVAGMLRSFAYAAAVAAQTDSSRDPAESERSMRKAFLTGYDPALETDAQRAGLLALFE
                     TEKVFYELRYELGSRPDWTWIPLAGIENLLSARPA"
     gene            7699..>9995
                     /gene="glgB"
                     /locus_tag="VGB85_22030"
     CDS             7699..>9995
                     /gene="glgB"
                     /locus_tag="VGB85_22030"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF003811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEY0136784.1"
                     /translation="MTPPPKSSRKATATTPTPAKSNPTPVAQPAKPAPAAQSPAPSSP
                     AATSQTAAQVKAGEAAPPITDSTTQATVTQTVTSPPVTPTVSQTQVTRPTTRPEDQTA
                     RAATPIAHALRDALTKGLSKVPHGILGPHPITQDGEPGIVVRAFHPNAITVECVLEAG
                     PRLPLHHEGGGLFSAFLPGASSTLRYHLRFEFPFGDPWEHADPYRFAPTIGPLDLHLL
                     GEGNHHRLWEVLGAHPRVLDGYTGTAFAVWAPNAQRVSVVGDFCAWDGRLFPMRELGA
                     GFFEIFIPDVHPGTLYKYELITREGLIRVKTDPCAASLEAPPSTASRIVRSDTYTWGD
                     AEWMQARRRQADPTRQPMAVYEVHLGSWAHEPTPDGKTRPLTYHELAARLPAYVKRLG
                     FTHIEVLPVAEHPFTGSWGYQVSGYFAPTARFGDPDGLRHFVDACHQHGLGVILDWVP
                     AHFPKDDFALRRFDGTALYEHEDPRLGEHPDWGTLIFNFGRPEVRNFLIANALYWLEV
                     FHFDALRVDAVASMLHLDYSRQPGQWLRNRLGGRENLEAIDFLKSLNTVIRTDQPGCF
                     TIAEESTSFPHVTGRPEDGGLGFTFKWNMGWMHDTLDYFSKDPIYRSHLHDKLTFGMM
                     YEYSERFVMPLSHDEVVHLKRSLVEKMPGDEWQRFANVRALLAYQYTRPGKKLLFMGS
                     ELGVPTEWNHDKGLDWDLLKDPMRAALHQYIARLGELYRDLPALWRGDPDPQAFAWLD
                     VHERERSILAYMRSDGDDHVIVVLNL"
BASE COUNT         1724 a         3878 c         2833 g         1560 t
ORIGIN      
        1 cgacctcgag acctttgaaa aggccctcgt cgccgacccg accctgctcg cgcacgccaa
       61 gttcgccgac atcttcgcca gccccgccga caacgtcctg gtcttcttcg ccgacctgct
      121 cggttatcaa gagccgtaca acgtccccgg caccgtcaac gacgacaact ggtccctgcg
      181 catcaccccc gactacgcca ctgcctacgc cgagcactgc cgccgacgca ccgcgctcga
      241 cctgccgacc gccctcgcca tggccctacg ccgcgccgac cagcccccct cacccgacct
      301 caccgaagtc ctcgccgccc tcgacgccct cggccaagcc tcaaccgcgc cttgagcttt
      361 gcaggcggac ggcgtagcgt catcgacgct cgccatgcgc ctggaacacg cacagatcag
      421 gaacttcaag gccattcgcg agctttcgtt gtctctcctg accacgcgtg gagacccccg
      481 cccactcacg ctcctgctcg gcgacaatgg ctccggcaag acgaccgctc tccaggcgat
      541 cgcgttgacc ctgtcgcttg cgacccggcg catcagcgcc ccggcaggcc tcgactggca
      601 aggcttcttg gccgagcgcg tcggctcgtt gggacccagc cgcgtcgcgc tcgacgtatg
      661 ttttgaagac gaggagttga agacgacgcg caacctcttc gacacgtgga tcgcacagca
      721 gcccctcgag ctccagcatc gcttcgcagc tcccggagat atgaaacgag tcgagctgat
      781 ctacgagaaa ggcgcactat tctccccgca aggccccggt gccatcagcc aatttctcgg
      841 cagacatttt atccgggatc tgtcgaagac caacgaggcc gcacgcgaca gcttctcgca
      901 gatcggtgac gtattctggt tcgaccaaaa ccgcaacccg acgaccacgg gtgccgagtt
      961 ggcgtgggat atgggcatta acaggctgcg cagcgagttg atccagtggt acgcctatca
     1021 tacatccagg cgtgtcattc cagccaacga ctatctgcaa aaattagagg atatgttcgc
     1081 ccggttcttc cccggcgcac gcttcgtcgg catcgagccg cgagaggtct tcgcgctcgg
     1141 cgatttcgag tcttacttcc tgatccagcg tggagaccgc gtgtacgaca tcgcggagat
     1201 gtcgtccggc gaacaggcga tcttccctct cctccacgag tttgcccgcc gaagcatcgc
     1261 ccgttcagtc gtcttgattg acgagctcga gctccacctg catccgccgt tgcaacaaat
     1321 gctcctctcg gccctgcgaa cgatcggccc tgactgccaa ttcatcgtca ctacccactc
     1381 gccgtacctc gaggaagcga cgcccgatga attcgagatc cgcctccccg gagggcggcg
     1441 atgcctctga gcctgctcta cgtcgagggc agcctcgacg tgatcctgat ggctggcgtc
     1501 gcgtcgaccc tctctgaggg cgccccttcg gtcgtctccg gcggctcaaa gggcgccctg
     1561 cgcccccggg tcatggaggc ccgtaacaaa ccacagccga ttgaggcctg ctatctgcga
     1621 gatcgcgact tcgacaccga cccgcccggg accctgaacg tacccaccgc tgataaaaac
     1681 gcgcaaggcg atgtcatcgg ctggcggtgg tgtcgccacg agatcgagaa ctacctgctc
     1741 gagcccagga tcgtcgcgca ggcgactggt tggaacgagg cggacttcat ccgctcgctg
     1801 ctcgcggcag cacgcgcttt acggtcctat actgccgccc gctgggctgt cggcaccgcg
     1861 cgtcgcagct tgcccccgct ctacgagctt tgcacccgcc cagagagcct gaagaacgag
     1921 atcaaggtcc ccggtgacct gtctgagcag gcgtgccgcc gctggatcac agagtacgtc
     1981 ggcgactttc attccaaggt ggcgctccaa ctcgccgata ccgcgctggc atcgaccttc
     2041 gagcattggt cacggcgcct ggatcgcgtg agcacgccac aggaagccct cctcgaccac
     2101 tccggcaagg acctcctggc ggcgctggag ccggagatcg cccaacgtcc tctcaataat
     2161 ccaatcgtgt tccgcaacgc gatccgcgac tggatcgtgg ccaatccggc gctggcgctg
     2221 gcacacctcc ccgaatgggc cgctctcctg cgcggcttcg cgtagccacc gcatttgccg
     2281 ggcctgccca ccatcagacc gcccgaaacg ctcgctcgtg cggcctgcgc gtgtgcgcca
     2341 ggccacctgc gtcatccccg cccatccgcc cgcgagacac ccctcgcccg gcaatttttc
     2401 ccccacccag cccccgggta acgttgtctt ccgctgcaca ggccgctctc tcgccgattc
     2461 ggcgcgcgct caccacggcc tccgcctcgc cacctcgccc cgcgccttgc cgaattccgg
     2521 caggccccac agggatgata cgcgccgccg ccggcgaacc tccccgggtg gcgcgaggcc
     2581 agtgccgtga accagccaat tcctactccg ccaagccccc gcgcacctgc accgatcatc
     2641 gtcgagggcg tgatgccgga gctcgacgcc gggcgttacg ccgtcaagcg ggtcgtcggt
     2701 gacgagctcg aggtcagcgc agcgatcttc aaggacggcc acgacttgct ggccgcgcgc
     2761 gtccgctacc gcggccccgg cgacaccgac tggcgcatga aacggatgcg ctacgacaat
     2821 gacgccgacc gctggtcgac gcacttcccg ctcgaccgcg tcggcacctg gaccttcgtc
     2881 gtcgaggcct ggaccgacgc cttcgccacc tggcgcagcg agctcgagaa gaagcacaac
     2941 gccgcccagg acatcacctc cgagctgctc gagggcgccc agctgctcga gcacaccgcg
     3001 tcgcgcgtcg agtatcccaa ggccaaggcc cagatgttgc gcctcgccgg gctgctgcgc
     3061 gacaccgact tcgagccgta cacgcgcgtg cgcgtcggcg tcgcgcccga gctgttggcg
     3121 gtgatggagg cgcactacat cccggtcgac ctcagcacct tcgagcgcga gatcaccgtg
     3181 cacgtcgacc gcgagcgggc ccgcttcgcc gcgtggtacg agttcttccc gcgctccgcg
     3241 accaccgacc ccaagcgcca cggcaccttc gccgacgcca ccaagatgct cgaccggatc
     3301 gcctggctcg gcttcgacgt cgtgtatctc ccgccgatcc acccgatcgg cgtcgccaac
     3361 cgcaagggcc ccaacaacac cctgacgccc ggcccggacg accctggcag cccgtgggcg
     3421 atcggcaacg agcacggcgg gcacacggcg atcgagccca agctcggcac gctcgaggac
     3481 ttcgaccgct tcgtcaagcg ggccggcgag ctcggcatcg agatcgccct cgactacgcc
     3541 ctccagtgct cgcccgacca cccttggctg aaagaacacc ccgagtggtt cttcgtccgc
     3601 cccgacggta cgatcaagta cgcagaaaat ccccccaaga agtaccagga tatctatccc
     3661 ctcaacttct ggtgtaagga ccgcgagggc ctgtggaacg cctgcctcga catctttttg
     3721 ttctggatcg ggcacggcgt tcgcaccttc cgggtcgata atccgcacac caaggcgctg
     3781 tcgttctggg agtgggtgat cgccgaggtc cgcaagcagc acccggacgt catcttcctc
     3841 gccgaggcct tcacccggcc caaccgcatg cgctacctcg ccaaggccgg gtttagccag
     3901 tcgtatacct acttcacgtg gcgcaatagc gccttcgaat tgcaggagta cctgaccgag
     3961 ctcacccaag gcccgatgcg cgagtacttc cgcggcaacc tgttccccaa cacccccgac
     4021 atcctccacg agttcctgca gcacggcggc cgcggggcgt tccgcgcccg cctgctgctc
     4081 gccgccacgc tgtcaccggt gtacggcatc tacagcggct tcgagctgtt ggaaaatacc
     4141 cccgtgcgtc ctggcagcga ggagtatctc gactccgaga agtaccagat ccgcgtccgc
     4201 aacttcgacg cgccgggcaa cctcaacgag gacatgcggc gcctgaacca gatccgccgc
     4261 gagaaccggg cgctgcagct gtacgacaac ctgcgctttc accactcgga gaacggcgcc
     4321 gtcctgttct accgcaagtc gttctttaaa aatgacctgc tgatcatcgt caactgcgat
     4381 ccccacaacc cgcaggagac gatggtgcac gtcccgctcg ccgagctcgg cctcaacgag
     4441 catacgccct ttgtcgtcga ggacctcttg accggcgcgc gatacacctg gcaaggcgtc
     4501 cgcaattacg tccgactcga cccaaagatc cagcccggcc acctgctgag gctcgttcgc
     4561 cccgaaggag agcagcacac atgaccatgg acccgctctg gtacaaggat gcgatcatct
     4621 accagatcca cctgaagtcg tactacgaca gcaaccgcga cggcttcggc gacttcgccg
     4681 gcctgatcca gaagctcgac tacattcagt cgctcggcgt caacaccctc tggctgctcc
     4741 ccttctaccc ctcgccgctg cgcgacgacg gctacgacat cgccgagtac aagggtatca
     4801 acccgcagta cggcacgctc gacgacttct cgcgcttcat ggaggaggcc caccgccgcc
     4861 acattcgcgt gatcaccgag ctcgtcgtca accacacctc ggaccagcac ccatggttcc
     4921 agcgcgcccg caacgccccc gccggctcgc ccgagcgcga cttctacgtg tggagcgaca
     4981 ccgccgagaa gtacaccgac gcgcgcatca tcttcaccga caccgagaag tcgaactgga
     5041 cctgggaccc ggtcgcgcgc cagttctact ggcatcgctt cttcagccac cagcccgacc
     5101 tcaacttcga caaccccgtc gtcaccgagg cgatcctcga tgtcatgcgc ttttggttcg
     5161 ccatgggcgt cgacggcatg cgcctcgacg ccgtgccgta cctgatcgag cgcgagggca
     5221 ccaactgcga gaacttgccc ggcacccacg cgatcctcaa gcagatccgc gccgcgctcg
     5281 acagcgagtt cgagggccgc ttgctgctcg ccgaggccaa ccagtggccg ccggacgtcg
     5341 tcccttactt cggcgacggc accgatgagt gccacatggc atttcacttc ccgctgatgc
     5401 cgcgcatgta tatcgcccag cgccgcgagg acgcgacgcc gatcatcgag atcatggagc
     5461 gcaccccgga gatccccccg cagtgccagt gggcgatctt cctgcgcaac cacgacgagc
     5521 tcaccctcga gatggtgacc gacgaggagc gcgactacat gtaccgcgag tacgctcgcg
     5581 acccgcgcat gcgcatcaac gtcggcatcc gccgccgcct cgcgccgctg atggacaacg
     5641 gccggcgcca gatcgagctg atgaacgccc tgctgatgtc gatgcccggc accccgatcg
     5701 tctattacgg cgacgagatc ggcatgggcg acaacgtcta cctcggcgac cgcaacggcg
     5761 tgcgcacccc gatgcaatgg accggcgacc gcaacgccgg cttctcgcag ggtgacaccg
     5821 ccgcgctcta cagcccgctg atcgtcgacg ccccgtacag ctaccacgcg atcaacgtcg
     5881 aggcgcagga gaagttcccg agctcgctgc tgcactggat gcgcagcctg atccgcgtgc
     5941 gcaagcaata caaggcgttc ggccgcggca cctgggagtc gctgccctcg gccaatcgtc
     6001 gcgtgctcgt gttcctgcgc aagtatgaga acgagacgct gctgtgcgtc aacaacctct
     6061 cgcgttacgt gcagtacacc gaggtcgacc tcgcacgctt cgacggctgc tccccgatcg
     6121 agctgttcag tgagggcccg ttcccgccga tcaccgacaa gccctatctg ctgaccctcg
     6181 gcccctactc gttcctgtgg ttccgcctgc tcaagaccga gaaggcgcag gagatggcga
     6241 tcccatgacc acgacccccg cgcacgacct cccgaccctc gagatctccg acaacctcgg
     6301 ccgcgccgcc ctcggcgcgc tcggccgcga ccagctcgcg gcgtacctct cggcccgacg
     6361 ctggttcggc gccaagggcc aggtgatcga ggccgccggc ttcgacgagg tcgccccgct
     6421 cgccgccggc gacgacgcga tcgcgctcgc ccgcatcgcc gtgcgcttcg ccagcggcca
     6481 gaccgagcac tatcagatcc ccctggcgat cgaccgcagc gggagcgtca gctcgctggt
     6541 gctcgccaac ctccacggcg gcggcgtgct ctacgacgcc gtcgagagcc ccgagttccg
     6601 ccgccagctc ggccgcgcct tcgcccacaa ggccaccgcc gtcgcacaga cagtccgctg
     6661 ggagttcgag ccgctcaccg acctcgccga catggccgac gccgagaccc gcatcgtcgg
     6721 cggcgagcag agcaacacct cgatcgtgtt cggcacccgc gcgatcatga agctgtttcg
     6781 ccacgtcgag accggcgaga accccgacgt cgagatcggc cgcttcctga ccacgcgcac
     6841 cagcttccgc ggcaccccgg ccctgctcgg cgtgctccac atgcgcggcc ccgacggcga
     6901 cggcgtcgcc ggcatggtcc aggagttcct ccccggatcc tccgacggct gggcccacgt
     6961 gctcgcccac ctcggcaagc acggcgccga cgagtcgttg atcacgcaac tgcaagccct
     7021 cggccgcctc acccgcgagc tgcacgaggc cctcgccagc gtctccgacg accccgactt
     7081 ctcccccgaa ccaaccgagc aggcccacct cgaccgctgg cgcaccgaca tcgatacgca
     7141 agtcagcacc accctgaccc tgctcggcac ccagaccaac aagctcccgc cgcagatcgc
     7201 caccgcggcc accgccctga tcgcccgcgg cgacgagctg cgcgcgctcc tcaagagctc
     7261 cacgggcgcc agcgcggccg gcccacgcat ccgccatcac ggcgattatc acctcggtca
     7321 ggtcctccac accgccgacg gcggctggcg catcatcgac ttcgagggcg agccggcgcg
     7381 cccgatctcc gagcgccgcg ccaaacacca cccgctgcgt gacgtcgccg gcatgctgcg
     7441 gtcgttcgcc tacgcggccg ccgtggccgc ccaaaccgac tcttcccgcg accccgccga
     7501 gtccgagcgg tccatgcgca aggcgttcct caccggctac gaccctgcgc tcgagaccga
     7561 tgctcagcgc gccggcctgc tcgccctctt cgagaccgag aaggtgttct acgagctgcg
     7621 ctacgagctc ggcagccgcc ccgactggac ctggatcccg ctcgccggga tcgaaaacct
     7681 cctgtccgca aggccagcat gacacccccg cccaaatcct cccgcaaagc gaccgcgacg
     7741 accccgaccc ccgcgaagtc gaatcccacg cccgtcgccc agcccgcaaa gccggccccc
     7801 gccgcacagt cacccgcccc gtcatcaccg gccgcgacct cccagaccgc cgcgcaggtc
     7861 aaggccggcg aggccgcccc accgatcacc gattcgacca cgcaggccac ggtcacgcag
     7921 accgtcacct cgcccccggt cacccccacc gtctcgcaga cccaggtcac gcgcccgacc
     7981 acgcgacccg aggaccagac cgcccgcgcc gcgacaccga tcgcccacgc gctgcgtgac
     8041 gcgctgacca agggcttatc aaaagttccg cacggcatcc tcggcccgca cccgatcacc
     8101 caggacggcg agcccggcat cgtcgtgcgc gcatttcacc caaatgcgat caccgtcgaa
     8161 tgcgtgctcg aggccggccc gcgcctgccg ctgcaccacg agggcggcgg cctgttctcc
     8221 gccttcctgc ccggggcctc ttcgaccctg cggtatcacc tccgcttcga gttccccttc
     8281 ggcgacccgt gggagcacgc cgacccctac cgcttcgccc cgacgatcgg cccgctcgac
     8341 ctccacctgc tcggcgaggg taatcatcac agattatggg aggtgctcgg cgctcacccg
     8401 cgcgtgctcg atgggtatac cggcaccgcc ttcgccgtgt gggctcccaa tgcccagcgc
     8461 gtcagcgtcg tcggtgactt ctgcgcctgg gacggccgcc tgttccccat gcgggagctc
     8521 ggcgccggct tcttcgagat cttcatcccc gacgtccacc ccggcacttt atataaatac
     8581 gagctgatca cccgcgaggg gctgatccgc gtcaagaccg acccctgcgc cgcctcgctc
     8641 gaggcgcccc cgagcaccgc ctctcgcatc gtccgcagcg acacctacac ctggggcgac
     8701 gccgagtgga tgcaggcccg acgccgccag gccgacccga cgcgccagcc gatggccgtc
     8761 tacgaggttc acctcggctc ctgggcccac gagccgacgc cggacggcaa gacccgcccg
     8821 ctcacctacc acgagctcgc cgcgcgcctg cccgcatacg tcaagcgcct cggcttcacg
     8881 cacatcgagg tcctcccggt cgccgagcac cccttcaccg ggtcgtgggg atatcaggta
     8941 tcggggtact tcgcgccgac cgcccgcttc ggcgaccccg acggcctgcg ccacttcgtc
     9001 gacgcctgcc atcaacacgg cctcggcgtg atcctcgact gggtgcccgc gcattttccc
     9061 aaagacgact tcgccctgcg ccgcttcgac ggcaccgccc tctacgagca cgaggacccg
     9121 cgcctcggcg agcacccgga ctggggcacg ctgatcttca acttcggccg ccccgaggtc
     9181 cgcaacttcc tgatcgccaa cgccctctac tggctcgagg tgttccactt cgatgccttg
     9241 cgcgtcgacg ccgtcgcctc gatgctgcac ctcgactaca gccgtcagcc tggccagtgg
     9301 ctgcgcaacc gcctcggcgg ccgcgagaac ctcgaggcga tcgacttcct caagtcgctc
     9361 aacaccgtca tccgcaccga ccagcccggc tgcttcacga tcgccgagga gtcgacctcg
     9421 ttcccgcacg tcaccggccg ccccgaggac ggcggcctcg gcttcacctt caagtggaac
     9481 atggggtgga tgcacgacac cctcgattat ttttcaaagg acccgatcta ccgcagtcac
     9541 ctgcacgaca agctgacctt tggcatgatg tacgagtaca gcgagcgctt cgtgatgccg
     9601 ctgtcccacg acgaggtcgt ccacctcaag cgctcgctcg tcgagaagat gccgggcgac
     9661 gagtggcaaa ggttcgccaa cgtgcgcgcc ctgctcgcct accagtatac gcgccccggc
     9721 aagaagctcc tgttcatggg cagcgagctc ggcgtgccga cggagtggaa ccacgacaag
     9781 ggcctcgact gggacctgct caaggacccg atgcgcgcgg cgctgcatca atacatcgcc
     9841 cgcctcggcg agctctaccg cgacctgccg gccctgtggc gcggcgaccc ggacccgcag
     9901 gccttcgcct ggctcgacgt gcacgagcgc gagcggtcga tcctcgcgta catgcgcagc
     9961 gacggtgacg accacgtgat cgtcgtcctc aacct
//
LOCUS       DASWNJ010001319         2089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_24383, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001319 DASWNJ010000000
VERSION     DASWNJ010001319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24383"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1089)
                     /locus_tag="VGB85_22035"
     CDS             complement(67..1089)
                     /locus_tag="VGB85_22035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136785.1"
                     /translation="MAMISRKKRSYRVSEGLFGFLKRHNRASEVPVSYTDLTRYDDTT
                     PVYDKRGRDTLWQSVLYPSSDRAQINQDLVRIYASLRVMGNTALMKHLVTDRVDVCTY
                     GNTQPFRVRILNTLNENFDYFYVKNADASRIYGLELEHLLSPNRVDFLSDGTTLIEEH
                     ISGIPGDQFIRDNLKDSHIDEVRLGKEFVKFNERCFLRLLGDMHSSNFVVNVIPDFDE
                     TMYRIRAIDFDQQSYEGRCNVYMPKYYSQNNAIIFLAIRCMTPESVRQYQEEEHTLLR
                     HRARAEHEALTELLSVMIQDELAPDDNIARLRQELAYHHRDDRFLTCTSMGSLVRASL
                     SLLEWE"
     gene            1215..>2089
                     /locus_tag="VGB85_22040"
     CDS             1215..>2089
                     /locus_tag="VGB85_22040"
                     /inference="COORDINATES: protein motif:HMM:NF015541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HEY0136786.1"
                     /translation="MHLRPLSLCLAAITLTTACGDDPGGDTGDSSGEPSTGADAGTTT
                     DTPTTDAPTTAESSGSSGDDTTGDDTGEAAFEVGGCGLPEYPLVATADMGQVLDHAFV
                     IEFEPATIDALLEGQGFGALTPVKYGARVYKIRYVTQDRGEAVEATGFVSLPVGATVE
                     ERPVVLWAHGTTGFTDKCAPTAAADGYVVPALLAALGYVSVAPDYLGMNGWGAASGFI
                     HPYIVPEPTAIATLDSLRALARFGVDTGEEMSATPGKDIILFGASEGGFATLWADRYA
                     PFYAPEFKILANVASV"
BASE COUNT          315 a          691 c          733 g          350 t
ORIGIN      
        1 cccccccccc cccccccccc ccgccgcgag gcaaattccg gccgaatgct cgtgtcccgc
       61 tctcgcctac tcccattcga ggaggctgag gctggcgcgc acgagggagc ccatcgaggt
      121 acacgtcaaa aaccggtcgt cgcggtggtg gtatgcgagc tcctggcgca gccgggcgat
      181 gttgtcgtcg ggggcgagct cgtcctggat catcacgctg agcagctcgg tgagggcctc
      241 atgttcggcg cgggcgcggt ggcgcagcag cgtgtgttct tcttcctggt actggcgcac
      301 cgactccggc gtcatgcagc gaattgccaa aaaaatgatc gcgttgttct gcgagtagta
      361 cttcggcatg tagacgttgc agcggccctc gtagctctgc tggtcgaagt cgatcgcgcg
      421 gatgcggtac atcgtctcgt cgaagtcggg gatgacattg acgacgaagt tgctcgagtg
      481 catgtcgccg agcaggcgta aaaagcagcg ctcgttgaac ttgacgaact ccttgccgag
      541 ccggacctcg tcgatgtgcg agtccttgag gttgtcgcgg atgaactggt cgccggggat
      601 ccccgagatg tgctcctcga tcagcgtcgt gccgtcggac aggaagtcga cgcggttggg
      661 tgacagcagg tgctcgagct cgagcccgta gatccgcgag gcgtcggcgt tcttgacgta
      721 gaagtagtcg aagttctcgt tgagcgtgtt gaggatccgg acgcgaaacg gctgggtgtt
      781 gccgtacgtg cagacgtcga cccggtcggt caccaggtgc ttcatcagcg ccgtgttgcc
      841 catgacgcgc aggctggcgt agatccggac caggtcctgg ttgatctgcg cgcggtccga
      901 cgagggatag agcaccgact gccacagcgt gtcgcggccg cgcttgtcgt agacgggggt
      961 cgtgtcgtcg tagcgggtga ggtccgtgta gctgaccggc acctccgagg cccggttgtg
     1021 tcgcttcagg aacccgaaga ggccctcgga gacccggtag ctccgcttct tgcgcgagat
     1081 catcgccatg cagcgcgcag tctgtcaggc cgctgtgtac ccggcaagcg ccggccgtgc
     1141 gccgagtcat gacgccgggc gccgaccggg gtctggcgca gccgcggtcg cctctgccaa
     1201 gatgcgcggt cgatatgcac ctccgtcccc tgtcgctctg tctcgccgcg atcaccctca
     1261 ccaccgcctg cggagacgac ccgggtggcg acaccgggga cagcagcggc gagccgagca
     1321 ccggcgcgga cgcggggacc acgaccgaca cgccgacgac ggacgcgccg acgacggccg
     1381 agagcagcgg cagctcgggt gacgacacga ccggcgacga caccggcgag gccgcgttcg
     1441 aggtcggtgg ttgtggcctg ccggagtacc cgctggtcgc caccgcggac atgggccagg
     1501 tgctcgacca tgcgttcgtg atcgagttcg agccggcgac gatcgacgcg ctgctcgagg
     1561 gccagggctt cggcgcgctg acgccggtga agtacggcgc gcgcgtgtac aagatccgct
     1621 acgtcacgca ggaccgcggc gaggccgtcg aggcgaccgg cttcgtcagc ctgccggtcg
     1681 gcgccacggt cgaggagcgc ccggtcgtgt tgtgggccca cgggacgacc ggcttcaccg
     1741 acaagtgcgc gccgacggcc gccgcggacg gctacgtcgt gccggcgctg ctcgccgcgc
     1801 tcggctacgt cagcgtcgcg ccggactacc tcggcatgaa cggctggggc gcggcgtcgg
     1861 gcttcattca cccgtacatc gtcccggagc cgaccgcgat cgccacgctc gactcgctgc
     1921 gcgcgctggc ccgcttcggc gtcgacaccg gcgaggagat gtcggcgacg ccgggcaagg
     1981 acatcatcct gttcggggcc tccgagggcg gcttcgccac gctgtgggcc gaccgctacg
     2041 ccccgttcta cgcgcccgag ttcaagatcc tcgccaacgt cgcctccgt
//
LOCUS       DASWNJ010001320         1676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_588846, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001320 DASWNJ010000000
VERSION     DASWNJ010001320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588846"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1488)
                     /gene="ccrA"
                     /locus_tag="VGB85_22045"
     CDS             complement(175..1488)
                     /gene="ccrA"
                     /locus_tag="VGB85_22045"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR01751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HEY0136787.1"
                     /translation="MSDAQAASGEAFTALDASVPDTKVIHDGTARAWQRIPTPGHTPE
                     TMLAYVIRREREGDLRRAFQVERVATPRPGPGEVVVLVMAAGINYNGIWAALGRPRSV
                     FDIHKHDLHIAGSDASGVVWEVGPGVKRWKPGDEVVIHGNMTCGECEACNGYDPMGCE
                     KHRVCGYETPYGSFAQMMVVQGQQLLRKPPKLTWEEAASYGVGCFTAYRMFDRANLRT
                     NETVLIWGAAGGLGCFAIQLARLQGAIPIAVVSNEEKAAVARLLGAEIVIDRTQFPDL
                     AYHRDESPEQTTRRLAATKAFGRTIQGALAERQIRSDGVDVVFEHTGQETFPASVFLA
                     KRMGRVVICGATSGYELTFDVRHLWMRQKSIIGCHGSNAQDSQRANELLCQGKIRPVL
                     SKAYTVDEFVLAHEEMRLNLKIGTTACLMVAPRLGLRNLAETLAA"
BASE COUNT          225 a          610 c          569 g          272 t
ORIGIN      
        1 ctcggcaccg cggtgatcct gaccggcatc tcggacatcg cccagacgct ggtcaacctc
       61 ggcatcaacc tcgcggcggt cgtcgtccgc gggacgctcg agagtggcat cgcccacgcg
      121 ctcgagcggg tcaaggaggg cgccgcgggg ccgcggcgcc gcggctgatc gccgtcaggc
      181 cgccagcgtc tcggccaagt ttcgcaggcc gaggcgcggc gcgaccatca ggcacgcggt
      241 cgtgccgatc ttcaggttga gccgcatctc ctcgtgcgcc agcacgaact cgtcgaccgt
      301 gtacgccttg ctcagcaccg gccggatctt gccctggcac agcagctcgt tggcgcgctg
      361 gctgtcctgg gcgttggagc cgtggcagcc gatgatgctc ttttgtcgca tccacaggtg
      421 gcgcacgtcg aaggtcagct cgtagccgct ggtcgccccg cagatcacga cgcggcccat
      481 ccgtttggcc aggaatacgc tcgcggggaa cgtctcctgc ccggtgtgct cgaacacgac
      541 gtcgacgccg tcggagcgga tctgccgctc cgccagcgcc ccctggatcg tgcgcccgaa
      601 cgccttggtg gccgcgagcc gccgcgtcgt ctgctcgggg ctctcgtcgc ggtggtaggc
      661 gaggtccggg aattgggtcc ggtcgatgac gatctcggcg ccgagcaggc gcgccactgc
      721 ggccttctcc tcgttgctga cgacggcgat cgggatcgca ccctgcagcc gcgcgagctg
      781 gatggcgaag cagccgagcc cgcccgctgc cccccagatc agcacggtct cgttggtccg
      841 gaggttcgcc cggtcgaaca tgcggtacgc cgtgaagcag ccgacgccgt acgaggcggc
      901 ctcctcccag gtcagcttcg gcggcttgcg cagcagctgc tggccctgga cgaccatcat
      961 ctgggcgaac gatccgtacg gggtctcgta gccgcagacg cggtgcttct cgcagcccat
     1021 cgggtcgtag ccgttgcagg cctcgcactc gccgcaggtc atgttgccgt ggatgacgac
     1081 ctcgtcgccg ggcttccagc gcttgacgcc gggcccgacc tcccacacca cgcccgaggc
     1141 gtcggagccg gcgatgtgca ggtcgtgctt gtggatgtcg aagaccgagc gcgggcgccc
     1201 gagcgccgcc cagatcccgt tgtagttgat gcccgcggcc atcacgagga ccacgacctc
     1261 gcccgggccc ggccgcggcg tggcgacgcg ctcgacctga aacgcgcgcc gcaggtcacc
     1321 ctcgcgctcc cggcggatca cgtacgcgag catggtctcg ggggtgtggc cgggcgtcgg
     1381 gatccgctgc cacgcgcgcg ccgtcccgtc gtgaatgacc ttcgtgtccg ggacgctcgc
     1441 atcgagcgcg gtgaaagctt cgccactggc tgcctgtgcg tccgacatga tgcctccctc
     1501 tatccctcgt cgcccacgac gctcgtcagg tacgcccgga ccatgcgggc cagccgcgat
     1561 actgtgggcg tctcgagcat cgagaagtga tcaccacgaa ctgtccacgc cgcgctctct
     1621 ccggcgcagc gatcgagcca gtactgctcc gcgcgccgct gctcggcgcc ctgcgg
//
LOCUS       DASWNJ010001321         2200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_516292, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001321 DASWNJ010000000
VERSION     DASWNJ010001321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516292"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VGB85_22050"
     CDS             <1..296
                     /locus_tag="VGB85_22050"
                     /inference="COORDINATES: protein motif:HMM:NF024736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0136788.1"
                     /translation="GGKPVDLRQVVDGIRWVLHTQAHWSDIPATYAATSTCWRWYKRW
                     CADGTWPKVEALLAAPPVKSARRPTPRALPPAPGSRTQRVPRVSGDPGCFTAS"
     gene            complement(1364..1798)
                     /locus_tag="VGB85_22055"
     CDS             complement(1364..1798)
                     /locus_tag="VGB85_22055"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEY0136789.1"
                     /translation="MTTSTTRFRSVLFLCVANSARSQMAEALARARFGDRVRVQSAGT
                     QPSQVNPNAVAVMAERGVDTARQHSKSVSTIDPATVDLVVTLCAEEVCPLFLGAATRL
                     HWPIDDPASSDPSISREEFLARFRRARDEIDARLDELTPEAP"
     gene            complement(1795..>2200)
                     /locus_tag="VGB85_22060"
     CDS             complement(1795..>2200)
                     /locus_tag="VGB85_22060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HEY0136790.1"
                     /translation="VTGRTLCAGIEIGSRFGLLATVVLLFDFQATTIVAQPLTIALIA
                     VQLIIQSYGIFGIAYLWARMWRLPHSIAAPAALIGTSNFFELAVAVAISLFGLQSGAA
                     LATVVGVLVEVPVMLSLVAFANRTARHFPAAA"
BASE COUNT          356 a          757 c          763 g          324 t
ORIGIN      
        1 gcggcggcaa gcccgtggat ctgcggcagg tcgtggacgg catccggtgg gtgctgcaca
       61 cgcaagcgca ctggtcggac atcccggcga cctatgccgc cacgtcgacg tgctggcgct
      121 ggtacaagcg gtggtgcgcg gacgggacct ggccgaaggt cgaggccctg cttgcggcgc
      181 cgccggtcaa gtcggcgcgc cggccgacgc cacgcgctct accacccgca ccagggagcc
      241 gtacccagcg agtgccccgt gtgtccggcg accccggctg ctttacggcg tcctgagtgg
      301 gcccacgacc ccttgctcac acgaaagggc cgtcttggcg ctgcgaggcg ctgtggacgg
      361 ccctccgttc gtccccgagg cggccatgac cgtccggcgc gccgcgcgaa cctgctccgc
      421 gagctgcgac atagctgcga cataactgca tcatgcctgc gccatgcctg cgccatgcct
      481 gccccatgcc tgccccatgc ctgcgtcata cctgcgctat gcctgcgcca taactgcgac
      541 gtaactgcga catcgccgat cgcggcgcgg agcgatgtcg gctaatctcg catggtaagg
      601 gcttctggtg ggtatacagg agagtttgga caacggccgt gcccccagag taacagccgc
      661 gaggcgagca aaccaccgca tggccgtgcc cgctggcacc accgaggcgg agggaccttc
      721 gccgagccgc ccacagtgcg gcgtcgcgcc tggtcggcct aatctgcgat cgcactgagc
      781 ccttgagcgg tgacagcctg cgaggcatcc tcggtcagcg agcactccaa actcctgcat
      841 ccgctcgcgg gcctttcgcg tctgtccagg catgaacccc accagccgca cctctgccgt
      901 cgagttcccc acgcgcctgc acgtgctgtc gtcgtgcgcg acgtggccgc cgcgctcccg
      961 ttctaccggg cccggttcgg cccggagccg accaagatct gcggccgtcc gggtcggtga
     1021 cccagaattt atcctggacc gcgtagcagc aggtcacggc cgcctcgccg tgcccgcttt
     1081 tagacgcgct cgacgcagcc gaggagcccg gctaagacgc ccagtccatc gccaggcgcg
     1141 gcgggctggg tcgggcgcca gtcacggcat catgcgcagc cgaggcccat cgccttgagc
     1201 gcatcgatat cgatagctcc ggcggcgcag cagtcggcca gcttgccgtc gatgacgacc
     1261 agggccgccg aggcgcgagc tgctgcggcc ttgtcgctgg acctcctcgc aggtcagcgg
     1321 ggccccaacc accgacttcg gcgacgctct gatattgacc gagctatggg gcttctggcg
     1381 tgagctcgtc gaggcgagcg tcgatctcgt cgcgggcgcg gcgaaagcgg gccaggaatt
     1441 cctcgcggga gatcgatggg tcggagctgg cggggtcgtc gatcggccag tgcagccgcg
     1501 tcgcggcgcc caggaacagg gggcacacct cctccgcaca cagcgtcacg acgaggtcga
     1561 ccgtcgcggg gtcgatggtc gacaccgact tggagtgctg cctggccgtg tcgacgccgc
     1621 gctcggccat cacggcgact gcgttggggt tgacctgcga gggctgcgtg ccggcgctct
     1681 ggacccgcac acggtcgccg aagcgggcgc gcgcgagggc ctcggccatc tggctgcgtg
     1741 ccgagttggc gacacacagg aacaggacgc tgcgaaagcg ggtcgtcgac gtggtcatgc
     1801 tgccgccgga aagtgacgcg cggtgcggtt ggcgaaggcg acgagcgaga gcatgaccgg
     1861 cacctcgacg agcacgccga cgacggtggc cagagccgcg ccggactgga ggccgaacaa
     1921 actgatcgct acggcgacgg ccagctcgaa gaagttggac gtgccgatga gagccgcggg
     1981 cgcggcgatc gagtgcggaa gccgccacat ccgggcccag aggtaggcga tgccgaagat
     2041 gccgtagctt tggatgatca actgcacggc gatcagcgcg atcgtcagcg gctgcgcgac
     2101 gatcgtggtc gcctggaaat cgaacagcag cacgaccgtg gccaggaggc cgaagcgcga
     2161 gcctatttca atcccggcac aaagcgtccg acccgtgaca
//
LOCUS       DASWNJ010001322         2191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_235283, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001322 DASWNJ010000000
VERSION     DASWNJ010001322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235283"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2191
                     /locus_tag="VGB85_22065"
     CDS             <1..>2191
                     /locus_tag="VGB85_22065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136791.1"
                     /translation="QWGDRDSAALLADLLRPPDAPALLLIASYRSEASNGGVVSALRE
                     AARGEVDMRSLDLGPLSEEEAADLALQLLAEKAPQLKGRARQIAHEAQGSPLFVRELV
                     RYAIAGGARPGEVSLDQVLLARLGALPPAARGLLEVVAVAGGPVDVRLAAAAANVQGE
                     DRTALATLRGEHLVLTGDENTVETYHDRIREVVLGTLGSEGIKDRHRQLAFAMEAAGD
                     RDSDALALHFREAGEWERAADYARMAAEQAAAGLAFDRAARLYRLALEFKPQSDPARG
                     ELTCRLADALANAGRGVKAAKLYLAAAAVMPAEAGLDLRRRATGQLLRAGQFTEGLAT
                     MRQVVAEVDLTIPTTPRGMVAALTYERARLKLRGLTLRERDPAIIDRADLAKLAVCRT
                     IASSISLHDPLVAALFHARHLNLALKTGHVFHAAIALATEVAHVSLRGEPGRERTERI
                     ATQATALAQRVPDALPRGMLALTRGIAAFHFGRWQEAIAALDRAEQILRGECVGTGYE
                     VTSAQSFRLVAIFLLGELRTLGTRMASFIAEARERDDTWADTILRAGPQVAYWLADDD
                     LPRARAELAYVQQRWPDDAPNVQRLWTFIGGIHIDFYGGDPATAWGRVAREWPDFARS
                     FLFQSQWARVMMSAMRAGAALGRLQSDHGESERTGLLRTVEADLDTLKGEKTLWATPF
                     VELVHAGLMIVHKQKDRAIHGLRAAEQGFRAVDMLLYAAAARRRCGQ"
BASE COUNT          285 a          837 c          792 g          277 t
ORIGIN      
        1 tccagtgggg cgaccgcgac agcgccgcgc tgctcgccga cctactgcgg ccgccggacg
       61 caccggcgct gctgttgatc gccagctatc gcagcgaggc gagcaacggc ggggtcgtca
      121 gcgcgctgcg agaggccgcg cgcggcgagg tcgacatgcg ctcgctcgat ctcgggccgc
      181 tctcggagga ggaggccgcc gacctcgcgt tgcagctgct cgccgagaag gcgccgcagc
      241 tcaagggccg cgcgcggcag atcgcccacg aggcccaggg ctcgccgttg ttcgtccgcg
      301 agctggtgcg ctacgcgatc gccggcgggg cccggcccgg cgaggtctcg ctcgaccagg
      361 tgctgctcgc ccgcctcggc gcgctgccgc ccgcagcacg cggcctgctc gaggtcgtcg
      421 ccgtcgccgg tgggcccgtc gatgtccgcc tcgccgccgc ggccgccaac gtgcagggcg
      481 aggaccgcac cgcgctggcg accctgcgcg gcgaacacct ggtgctcacc ggcgacgaga
      541 acaccgtcga gacctatcac gaccgcatcc gcgaggtcgt gctcggcacg ctcggcagcg
      601 agggcatcaa ggaccgccac cgccagctcg cgtttgcgat ggaggccgcg ggcgaccgcg
      661 actcggacgc gctggcgctg cactttcgcg aggccggcga gtgggagcgg gccgccgact
      721 acgcgcgcat ggccgcggag caggcggcgg cgggcctggc cttcgaccgc gcggcccggc
      781 tgtatcgcct cgccctcgag ttcaagccgc agagcgaccc tgcgcgcggc gagctgacgt
      841 gccgcctcgc ggacgcgctg gccaacgcgg gacgcggggt caaggcggcc aagctgtacc
      901 tcgccgcggc cgcggtgatg cccgccgagg ccggcctcga cctgcgccgg cgggccaccg
      961 gacagctgct gcgcgcgggc cagttcaccg agggcctggc gaccatgcgc caggtcgtcg
     1021 ccgaggtcga cctgacgatc ccgacgaccc cgcggggcat ggtggcggcg ctgacctacg
     1081 agcgcgcccg cctcaagctg cgcggcctga ccctgcgcga gcgcgacccg gcgatcatcg
     1141 accgcgccga cctggcgaag ctcgccgtgt gccgcacgat cgccagcagc atctcgttgc
     1201 acgacccgct ggtggccgcg ctgttccacg cgcgccacct caacctggcc ctcaagacag
     1261 gccacgtgtt ccacgccgcg atcgccctcg ccaccgaggt cgcgcacgtg tcgctgcgcg
     1321 gcgagccggg ccgcgagcgc accgagcgga tcgcgaccca ggcgaccgcg ctcgcccagc
     1381 gggtcccgga cgcgctgccc cgcggcatgc tggcgctgac ccgcgggatc gcggcgtttc
     1441 acttcggccg ctggcaggag gcgatcgcgg cgctcgaccg cgccgagcag atccttcgcg
     1501 gcgagtgtgt cggcaccggc tacgaggtga cgagcgcgca gtccttccgc ctggtggcga
     1561 tcttcctgct cggtgagctg cgcacgctcg gcacgcgcat ggcgagcttc atcgccgagg
     1621 cccgcgagcg cgacgacacc tgggccgaca cgatcctgcg cgccgggccg caggtcgcct
     1681 actggctggc cgacgacgac ctcccgcgcg cccgcgccga gctcgcgtac gtgcaacagc
     1741 gctggccgga cgacgccccg aacgtgcagc gcctgtggac cttcatcggc gggatccaca
     1801 tcgacttcta tggcggtgat ccggcgaccg cgtggggtcg cgtcgcccgc gagtggccgg
     1861 acttcgcccg cagcttcctg ttccagagcc agtgggcccg cgtgatgatg tctgccatgc
     1921 gcgccggcgc ggcgctcggt cgcctgcagt cggaccacgg cgagagcgag cggaccggcc
     1981 tgctgcgcac cgtcgaggcc gacctcgaca cgctcaaggg cgagaagacc ctgtgggcga
     2041 cccccttcgt cgagctggtc cacgccggcc tgatgatcgt gcacaaacaa aaagatcgcg
     2101 cgatccacgg cctgcgcgcg gcggagcagg gctttcgcgc cgtcgacatg ctgctctacg
     2161 ccgccgccgc gcgccgccgc tgcgggcagt t
//
LOCUS       DASWNJ010001323         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_483004, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001323 DASWNJ010000000
VERSION     DASWNJ010001323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483004"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VGB85_22070"
     CDS             complement(<1..267)
                     /locus_tag="VGB85_22070"
                     /inference="COORDINATES: protein motif:HMM:NF014494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEY0136792.1"
                     /translation="MKIVLGITGASGATYARRAVQLLSALRSGALPGVPARPGLEVDV
                     VASATAPQVWRHELGVDMPAQWDGLRRWDRGDFNAPFASGSNAPD"
     gene            complement(264..1025)
                     /locus_tag="VGB85_22075"
     CDS             complement(264..1025)
                     /locus_tag="VGB85_22075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HEY0136793.1"
                     /translation="MTSLSQLETPAVTLVDAHGAQVQSMFDGIAHGYDRANRWMSFGT
                     DIRWRRKAVAGMLPAGRDPHGARLLDLCAGTLDSTLEIHQQYPAADILAGDFSAGMLA
                     HGAGKLTGAAAQRITTQQMDAHALPLADASHDAIFCAFGIRNLSDLEVATCEQLRVLR
                     PGGRLTILEFFRPTAAITRAVHALHNRTLLPLLGWAATGKLGAYLYLPRSIGRFTTVE
                     SYTQQLRDCGFVDVQVERLTLGIAWVVRASRPETA"
     gene            complement(1044..>1501)
                     /locus_tag="VGB85_22080"
     CDS             complement(1044..>1501)
                     /locus_tag="VGB85_22080"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY0136794.1"
                     /translation="TSALIAWCASLGGLVEPRIAAALHRYGLEIGYAFQIADDVLDYT
                     SGVDQTGKARGQDLRDGKLTLPMILACSHDAGLRRRLMQLLHAGPPVDLDAATDLVTR
                     VGASSAVEQASAVAEAHAATAIAQLAVLPPSPAREALAELARYVARRRT"
BASE COUNT          200 a          464 c          647 g          190 t
ORIGIN      
        1 gtcgggggcg ttggagccgg aggcgaaggg ggcgttgaag tcgccgcggt cccagcggcg
       61 caggccgtcc cactgcgcgg gcatgtcgac gccgagctcg tgccgccaca cctgcggggc
      121 ggtcgcggag gcgacgacgt cgacctcgag gccggggcgc gcgggcacac ccggcagcgc
      181 gcccgagcgc agcgcggaca gcagctgcac ggcgcggcgg gcgtaggtcg caccgctcgc
      241 gccggtgatg ccgagcacga tcttcatgcg gtctccggcc ggctcgcgcg cacgacccag
      301 gcgatgccga gggtcaggcg ctcgacctgc acgtcgacga agccgcagtc acgcagctgc
      361 tgcgtgtacg actcgacggt ggtgaagcgg ccgatgctgc gcgggaggta gaggtaggcg
      421 ccgagcttgc cggtcgcggc ccagccgagc agcggcagca gggtgcggtt gtgcagcgcg
      481 tgcacggccc gggtgatcgc ggcggtcggg cggaagaact cgaggatcgt caggcgaccg
      541 ccgggacgca ggacgcggag ctgctcgcag gtggcgacct cgagatcgct gaggttgcgg
      601 atcccgaagg cgcagaagat cgcgtcgtgg ctggcgtcgg cgagcgggag cgcgtgggcg
      661 tccatctgct gggtcgtgat gcgctgcgcg gcggcgccgg tgagcttgcc ggcgccgtgc
      721 gccagcatgc cggccgagaa gtcgccggcg aggatgtcgg cggcgggata ctgctggtgg
      781 atctcgaggg tcgagtcgag ggtgccggcg cacaggtcga gcaggcgggc gccgtgcgga
      841 tcgcggccgg caggcaacat gccggcgacg gccttgcggc gccagcggat gtcggtgccg
      901 aagctcatcc agcggttggc ccggtcgtag ccgtgggcga tgccgtcgaa catgctctgc
      961 acctgggcgc cgtgggcatc gacgagggtg acggcgggag tctcgagctg gctgaggctg
     1021 gtcatgggga caggtccgat cggtcaggtg cggcggcggg cgacgtagcg ggcgagctcg
     1081 gcgagcgcct cgcgggcggg ggagggcggc aacacggcga gctgggcgat cgcggtcgcg
     1141 gcgtgggcct cggcgacggc ggaggcctgc tcgacggccg aggaggcgcc gacgcgggtc
     1201 acgagatcgg tggcggcgtc gaggtcgacc ggcggaccgg cgtggagcag ctgcatcagg
     1261 cgccggcgca ggccggcgtc gtggctgcag gccaggatca tcggcagggt cagcttgccg
     1321 tcgcgcaggt cctggccgcg ggccttgccg gtctgatcga cgccgctggt gtagtcgagc
     1381 acgtcgtcgg cgatctggaa ggcgtagccg atctcgaggc cgtagcggtg cagcgcggcg
     1441 gcgatgcgcg gctcgacgag gccgccgagg ctggcgcacc aggcgatcag cgcggaggtc
     1501 t
//
LOCUS       DASWNJ010001324         1866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_163448, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001324 DASWNJ010000000
VERSION     DASWNJ010001324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163448"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VGB85_22085"
     CDS             <1..838
                     /locus_tag="VGB85_22085"
                     /inference="COORDINATES: protein motif:HMM:NF023454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HEY0136795.1"
                     /translation="LKGEKVAELKAAGVEYEERMAELEKISYPKPNADFIYDTFNEFV
                     LRHPWVARESIRPKSIVREMVENFSAFNDYVKDYSLQRSEGVLLRYLMQAYKVLAQTV
                     PEAAKTDEVHDIIGFLRAMFAHVDTSLLQEWERMLHPPAEIAGPEKVGPQHAGEDKKG
                     FAARVRAEMHALVRALGQRDWDVAAAAVQQDPEDLWPASRFESAMVEYFAEYPELRFD
                     HGARFPKYTIIEHESDRVWRVRQVLVDPEGDGAWAIEGTVDLRNDPFPEGPMVRVRSI
                     GV"
     gene            complement(842..>1866)
                     /locus_tag="VGB85_22090"
     CDS             complement(842..>1866)
                     /locus_tag="VGB85_22090"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEY0136796.1"
                     /translation="CRTIRESAPLEDQHRFDRHAEGMLLSLCYAASLGGTATPVGTAP
                     NVIALDLLERQALVRIDFFQWMSFALPTALASLGVVLLYAWWRFPSPVRRLDRLTETV
                     EAELAQLGPLSRGERRVAGIFFVAVAGWLTPSLLKLSLGAAHPWTAWAAGGLDEGVVA
                     LLAGILLCLTPNGRGEGKPLLAWDRAMQLDWGTLLLLGGGLSLGKLMFDTGLAKAVGT
                     GVIEAAGPLASSPLGLLGCSTFLVLYLTEITSNVATTSMMVPVLLAIAQAGGNDPVPT
                     TLCVTMAASYAFMLPVSTPPNAIAYGTGKIRIGAMIRFGFVLDILGFIIIFACGALLI
                     PHLNFG"
BASE COUNT          341 a          595 c          676 g          254 t
ORIGIN      
        1 gctcaagggc gagaaggtcg ccgagctcaa ggccgcgggc gtcgagtacg aggagcgcat
       61 ggcggagctc gagaagatct cgtaccccaa gcccaacgcc gacttcatct acgacacttt
      121 taatgagttc gtgctgcgcc acccgtgggt cgcgcgcgag agcatccgcc ccaagtcgat
      181 cgtgcgcgag atggtcgaga acttctcggc gttcaatgat tatgtgaagg attacagcct
      241 gcagcgcagc gagggcgtgc tgctgcgcta cctgatgcag gcctataaag tcctcgcgca
      301 gacggtgccc gaggccgcca agaccgacga ggtccacgac atcatcggct ttttgcgagc
      361 catgttcgcc cacgtcgata ccagcctgct gcaggagtgg gagcgcatgc tgcacccgcc
      421 cgccgagatc gcagggcccg agaaagttgg gccacagcac gccggcgagg acaagaaggg
      481 cttcgcggcg cgtgtgcggg ccgagatgca cgcgctcgtc cgggcgctcg ggcagcgcga
      541 ctgggacgtc gccgccgccg cggtacagca ggatccggag gacctgtggc cggcgagtcg
      601 ctttgaaagc gcgatggtcg agtacttcgc ggagtacccg gagctgcgct tcgaccacgg
      661 ggcgcgcttt ccgaaataca cgatcatcga gcacgagagc gaccgcgtgt ggcgcgtgcg
      721 tcaggtgctc gtcgaccccg agggcgacgg cgcgtgggcg atcgagggca ccgtcgacct
      781 gcgcaacgac cccttccccg aggggccgat ggtgcgggtg cgcagcatcg gcgtgtgacg
      841 atcagccgaa gttgaggtgc gggatcagca gggcgccgca ggcgaagatg atgatgaagc
      901 cgaggatgtc gagcacgaag ccgaagcgga tcatcgcgcc gatccggatc ttgccggtgc
      961 cgtaggcgat cgcgttcggc ggggtcgaga ccgggagcat gaacgcgtag ctcgcggcca
     1021 tcgtcacgca cagcgtggtg gggaccgggt cgttgccgcc ggcctgcgcg atcgccagca
     1081 gcaccgggac catcatgctc gtcgtcgcca cgttgctggt gatctcggtc aggtacagca
     1141 ccaaaaacgt gctgcagccg agcaggccga gcggcgagga ggcgagaggg cccgcggcct
     1201 cgatcacacc ggtgccgacc gccttcgcaa gcccggtgtc gaacatcagc ttgccgagcg
     1261 agaggccgcc gccgagcagc aagagcgtgc cccagtcgag ctgcatcgcg cggtcccagg
     1321 cgagcaatgg cttgccctcg ccgcggccgt tcggggtcag gcacagcagg atgccggcga
     1381 gcagcgcgac gacgccctcg tcgaggccac ccgccgccca cgcggtccac gggtgagctg
     1441 ccccgagaga cagttttaat aagctcggcg tcagccagcc ggccacggcg acgaagaaga
     1501 taccggcgac gcgacgctcg ccgcgcgaca gcggtccgag ctgcgcgagc tcggcctcga
     1561 ctgtttcggt cagtcggtcg agccggcgca ccggcgaggg gaagcgccac cacgcgtaca
     1621 gcaggaccac gccgagcgag gcgagcgcgg tcggcagggc gaagctcatc cattgaaaaa
     1681 aatcgatgcg gacgagggcc tggcgctcga gcaggtcgag ggcgatcacg ttgggcgccg
     1741 tgccgacggg cgtggcggtg ccaccgagcg aggcggcgta gcacagcgac agcagcatgc
     1801 cctcggcgtg gcggtcgaag cggtgctggt cctcgagcgg cgcggactca cgaatggtcc
     1861 ggcaca
//
LOCUS       DASWNJ010001325         2301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_59529, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001325 DASWNJ010000000
VERSION     DASWNJ010001325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59529"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VGB85_22095"
     CDS             <1..766
                     /locus_tag="VGB85_22095"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0136797.1"
                     /translation="RKKPTPKLDVDTDGDGILDRLDKCVKVPGVAPDGCPLPPPAEEP
                     DTDGDGFKDSVDACVDVPGTGPDGCPDTDGDGFKDNVDKCPQVAGVAPDGCPPPDTDG
                     DGIIDANDKCINEPESKNNYQDADGCPDEVPKAVAKFSGVIKGIYFDVNKDTIKSNSA
                     ATLNSAVKVLKEFPDVNVEISGHTDSDGDRDYNVDLSRRRAESVKKFLVDKGIESNRL
                     HTRGVGPDEPVADNKTKKGKALNRRIEFKLMNELSK"
     gene            complement(898..>2301)
                     /locus_tag="VGB85_22100"
     CDS             complement(898..>2301)
                     /locus_tag="VGB85_22100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0136798.1"
                     /translation="LVAAEILCRAKGDEFCRFIMAHPTHIEGYIQAYFQSQPNLVRHT
                     ARYETPGFFSRKKQEDELRNREEQYRGIFEAGTNALLILADDGQIVQANPAATDLFRS
                     DAGPALPGSTVADLITDPRFFADFRARIHEHGHYNAEKIAVARDGQRYHVEIRGSRFR
                     FQNRDHLLAIVNDISEQKVAQIALRAAHDELEGRVRDRTAELESVNQQLKLVNEKLVT
                     ARDHAIDASKAKSAFLANMSHELRTPLNAIIGYSELIEEESRDAEQQSLTDLGKIRAA
                     ARHLLALIDDILDVSKIEAGKMDLLVESFAARELLEEIVATIQPLTARNRNTLVLDLD
                     PDPGALGVLVMDRTKLKQIALNLLSNACKFTHDGQVRLSVHRTRGPGGEWLHLKVEDS
                     GIGIPQERLEELFQPFRQADESTTRKYGGTGLGLSISRHYCQMMRGSIQAVSSPGGGS
                     TFSVQLPVDISRPRLRA"
BASE COUNT          370 a          740 c          825 g          366 t
ORIGIN      
        1 ccgcaagaag ccgaccccca agctcgacgt cgacaccgac ggcgacggca tcctcgaccg
       61 cctcgacaag tgcgtcaagg tgcccggcgt cgcgcccgac ggctgcccgc tgcccccgcc
      121 cgccgaggag ccggacaccg acggcgacgg cttcaaggac agcgtcgacg cctgcgtcga
      181 cgtgcccggc accgggccgg acggctgccc cgacaccgac ggcgacggct tcaaggacaa
      241 cgtcgacaag tgcccgcagg tcgccggcgt cgcgcccgac ggctgcccgc cgcccgacac
      301 cgacggcgac ggcatcatcg acgccaacga caagtgcatc aacgagcccg agtccaagaa
      361 caactaccag gacgccgacg gctgcccgga tgaggtcccc aaggccgtcg ccaagttcag
      421 cggcgtcatc aagggcatct acttcgacgt caacaaggac acgatcaagt ccaactcggc
      481 ggcgaccctc aacagcgccg tcaaggtgct caaggagttc ccggacgtca acgtcgagat
      541 cagcggccac accgacagcg acggcgaccg cgactacaac gtcgacctct cgcgccgccg
      601 cgccgagtcg gtcaagaagt tcctcgtcga caagggcatc gagtccaacc gcctgcacac
      661 gcgtggcgtc ggtcccgacg agccggtcgc cgacaacaag accaagaagg gcaaggccct
      721 caaccgccgc atcgagttca agctcatgaa cgagctctcg aagtaactcg tcccgctccg
      781 ggcacgcccg gtggcgtaca caggcgctcg atccctcagg atcgggcgct ttttcgttcg
      841 gtgggcctcg gggtgctcac cagcctgcgt tgcgcgtcgc aggccgtgca agccacgcta
      901 ggcgcgcagg cggggccggc tgatgtcgac gggcagctgg acgctgaagg tcgatccgcc
      961 gcccggcgag ctgacggcct ggatcgatcc acgcatcatc tggcagtagt gccggctgat
     1021 cgacaggccg aggccggtgc cgccgtactt gcgggtggtc gactcgtcgg cctggcgaaa
     1081 cggctggaac agctcttcca ggcgctcttg cgggatgccg atgccggagt cctcgacctt
     1141 gagatgcagc cattcgccgc cggggccacg ggtgcggtgg acggacaggc ggacctggcc
     1201 gtcgtgggtg aacttgcagg cgttgctgag caggttgagg gcgatctgct tgagcttggt
     1261 gcgatccatg accagcacgc cgagggcgcc ggggtcgggg tcgaggtcga ggacgagggt
     1321 gttgcggttg cgggcggtca gcggctggat cgtggcgacg atctcctcga gcagctcgcg
     1381 ggccgcgaag gactcgacga ggaggtccat cttgccggcc tcgatcttcg agacgtcgag
     1441 gatgtcgtcg atgagggcga gcaggtggcg cgcggcggcg cggatcttgc cgaggtcggt
     1501 gaggctctgc tgctcggcgt cgcggctctc ctcctcgatc agctcgctgt aaccgatgat
     1561 cgcgttgagc ggcgtgcgca gctcgtggct catgttggcc aggaaggcgc tcttggcctt
     1621 gctggcgtcg atcgcatggt cgcgggcggt gaccagcttc tcgttgacca gcttgagctg
     1681 ctggttcacc gactcgagct cggcggtgcg gtcgcggacg cggccctcga gctcgtcgtg
     1741 tgcggcgcgc agggcgatct gggcgacctt ttgttcgctg atgtcgttga cgatcgccag
     1801 caggtgatcg cggttttgaa agcggaagcg agagccgcgg atctcgacgt ggtagcgctg
     1861 gccatcgcgg gcgacggcga tcttctccgc gttgtagtgg ccgtgctcgt ggatccgggc
     1921 gcgaaagtcc gcgaaaaagc gcgggtcggt gatcaggtcg gcgacggtgg agccgggcag
     1981 ggcggggccg gcgtcggacc ggaacaggtc ggtcgcggcg gggttggcct gcacgatctg
     2041 gccgtcgtcg gcgaggatca gcagcgcgtt ggtgccggcc tcgaagatcc cgcggtactg
     2101 ctcctcgcgg ttgcgcagct cgtcctcttg cttcttgcgc gagaagaagc cgggggtctc
     2161 gtagcgcgcc gtgtggcgca cgaggttggg ctggctctga aagtaggcct ggatgtagcc
     2221 ctcgatgtgg gtcgggtggg ccatgatgaa gcggcagaac tcgtcgccct tggcgcggca
     2281 caggatctcc gcggcgacca g
//
LOCUS       DASWNJ010001326         3085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_307039, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001326 DASWNJ010000000
VERSION     DASWNJ010001326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307039"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VGB85_22105"
     CDS             <1..336
                     /locus_tag="VGB85_22105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136799.1"
                     /translation="MAGMASLEQQGATLDSEGCVDRVLAWHKQCDALSVMCNDAVKIA
                     MFHCLEARDRTDDCGKLKPDVVSKGQWVFAVCKDRGTQCRTRKQCACADAYRALDSFC
                     RTDQQAVQL"
     gene            336..1319
                     /gene="cyoE"
                     /locus_tag="VGB85_22110"
     CDS             336..1319
                     /gene="cyoE"
                     /locus_tag="VGB85_22110"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEY0136800.1"
                     /translation="MNEATHTAPGSAPAPRKQRRLLHDLMTLTKPGVTRMCALTAAGA
                     AWMALRSSDNPFGAETPADGWCSPGFAVAFAGVVGASLAVAGASALNMWLERAKDPLM
                     ARTKTRPLALPLAKRQLSPGTALGFAVTLCVTAIAVLWLFTNPLTAMLAAWAIVGYAL
                     VYTPLKYRTPLALVIGAVPGAAPPLLGWTAVTGDIDAGGLALFAILLVWQMPHFLAIT
                     LFRESDFARAGIQCVPVVRGSLVTRIQSVLWSIVLVPISLLPTVIGLTGYLYGAVALA
                     LSLAFLACACTGMQVFGSKADELRWARRFFFASLLYLPALIAALVVDVLQT"
     gene            1332..2102
                     /locus_tag="VGB85_22115"
     CDS             1332..2102
                     /locus_tag="VGB85_22115"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY0136801.1"
                     /translation="MRPDADMPPLEERGGWLQRAFVRRPWHFAAGFLVVVITASRPFM
                     RNVPEAPPVLLQVPDFALQDQDGQPFTLASMRGKVWVAGFIFTTCPSTCPKISRAMLE
                     LQQRYARNGVDVELVSFTVDPENDTPAVLARYAENLGADQSHWRFVTGDRKAMEDLVV
                     GGFKTGMDRIPDPTRVDMYDVAHSEKLVLIDGEGGIRGFYGVRHPKGAPVEPLPMGVH
                     ETPEARALGLDVLSLDELYHRSTHVLRDQNKKNGGCNR"
     gene            2090..2554
                     /locus_tag="VGB85_22120"
     CDS             2090..2554
                     /locus_tag="VGB85_22120"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY0136802.1"
                     /translation="MQPLISPRVILVAGLWVLSFGTACEGGPPPFTGPRTLLRTGEAP
                     LEVSAELLNDGQKLFNRYCASCHGYEGGGDGPSARSLDPKPRDFRAAQFLYSSAGEGS
                     LPTDAELKSTIQNGVVKRGMPAWKGMNADDLDALVSYIKTFSPRWQPPAAAP"
BASE COUNT          435 a         1154 c         1017 g          479 t
ORIGIN      
        1 atggccggca tggcctcgct cgagcagcag ggcgccacgc tcgacagcga gggctgcgtc
       61 gaccgggtgc tcgcctggca caagcagtgt gatgcgctgt cggtgatgtg caacgacgcc
      121 gtcaagatcg ccatgttcca ctgcctcgag gcgcgcgacc ggaccgacga ctgcggcaag
      181 ctcaagcccg acgtcgtgtc caaggggcag tgggtgttcg cggtctgcaa agatcgcggc
      241 acacagtgcc ggacccgcaa gcaatgcgcc tgcgccgacg cctatcgcgc gctcgacagc
      301 ttttgtcgca ccgaccaaca ggccgtgcaa ctctgatgaa cgaagccacg cacaccgccc
      361 ccggctctgc ccccgcgcct cgcaagcagc gtcgactgct gcacgacctg atgacgctga
      421 cgaagccggg cgtcacccgc atgtgcgccc tgacggccgc cggcgccgcg tggatggccc
      481 tgcgcagcag cgacaacccc ttcggcgccg agaccccggc cgacggctgg tgctcgccgg
      541 gcttcgccgt cgcgttcgca ggcgtcgtcg gggccagcct cgccgtcgcc ggcgcgagcg
      601 ccctcaacat gtggctcgag cgggccaagg acccgctgat ggcgcgcacc aagacccgcc
      661 cgctcgcctt gccgctcgcc aagcgccagc tgtccccggg gacagctctc ggcttcgccg
      721 tcaccctctg cgtcaccgcg atcgcggtgc tgtggctgtt caccaacccg ctgacggcga
      781 tgctcgccgc gtgggcgatc gtcggctacg cgctggtcta caccccgctc aagtaccgca
      841 ccccgctcgc gctggtgatc ggcgccgtgc ccggtgccgc cccgccgctg ctcggctgga
      901 cggccgtcac cggggacatc gacgccggcg gcctcgcgct gttcgcgatc ctgctggtct
      961 ggcagatgcc gcacttcctc gcgatcacgc tgtttcgcga gtcggacttc gcccgcgcgg
     1021 ggatccagtg cgtgccggtc gtgcgcggct cgctggtcac ccggatccag tccgtgctgt
     1081 ggtcgatcgt gctggtgccg atctccctgc tgccgaccgt gatcggcctc accggttacc
     1141 tctacggcgc cgtcgccctc gcgctgagcc tcgccttcct cgcgtgcgcc tgcacgggca
     1201 tgcaggtgtt cggcagcaag gccgacgagc tccgctgggc ccgtcgcttc ttcttcgcct
     1261 cgctcctcta cctcccggcg ctgatcgccg cgctcgtggt cgacgtgctc cagacctgat
     1321 gcgacgacgc catgcgacct gacgccgaca tgcctccact ggaggagcgc ggaggctggc
     1381 tgcagcgcgc cttcgtgcgc cgcccgtggc acttcgccgc cggcttcctg gtggtcgtca
     1441 tcaccgcgag ccggccgttc atgcgcaacg tgcccgaggc cccgccggtg ctcctgcagg
     1501 tcccggactt tgccctgcag gaccaggacg gccagccctt caccctcgcc agcatgcgcg
     1561 gcaaggtctg ggtcgccggc ttcatcttca ccacctgccc gagcacctgc ccgaagatct
     1621 cgcgcgcgat gctcgagctg cagcagcgtt acgcccgcaa cggcgtcgac gtcgagctcg
     1681 tcagcttcac cgtcgacccc gagaacgaca cccccgcggt gctcgcccgc tacgccgaga
     1741 acctcggcgc cgaccagagc cactggcgct tcgtcacggg tgaccgcaag gcgatggagg
     1801 acctcgtcgt cggcggcttc aagaccggca tggaccggat ccccgatccg accagggtgg
     1861 acatgtacga tgtcgcgcac tccgaaaaac tggtcctgat cgacggggag ggcgggatcc
     1921 gcggcttcta cggggttcgc cacccaaagg gcgccccggt cgagcccctg ccgatgggcg
     1981 tgcacgagac cccggaggcc agggcgctcg gcctcgacgt gctctcgctc gacgagctgt
     2041 accaccgctc gacccacgtg ctgcgcgacc agaacaagaa aaacggcggg tgcaaccgtt
     2101 gatatccccc cgcgttatcc tcgtagctgg cctttgggtc ctgtccttcg ggacagcctg
     2161 cgagggcgga cctccgccgt tcaccgggcc tcgcacgctg ctgcgcaccg gtgaggcgcc
     2221 gctcgaggtc tccgccgagc tgctcaacga cggccagaag ctgttcaacc gctactgcgc
     2281 ctcgtgccac ggctacgagg gtgggggcga cggcccctcc gcgcgcagcc tcgaccccaa
     2341 gccgcgcgac ttccgggccg cgcagttcct gtacagcagt gcaggcgagg gcagcctgcc
     2401 gaccgacgcc gagctcaagt cgacgatcca gaacggcgtc gtcaagcgcg gcatgccggc
     2461 ctggaagggc atgaacgccg acgacctcga cgcactggtg agttacatca agaccttctc
     2521 gccgcgctgg cagccgcccg cggcggctcc ctgagcacct gtcccgatgg acagccggcg
     2581 tcggggcccc gggagatcca cccgacgttt tcgtgcctgc gcgcacgtac agggcgctga
     2641 ctgggccatt caccgtgcgt ggcgaccgcc aggtttgcgg tccacgaagt gtccacgtgt
     2701 ccggggcgcc tgcgggcgca ccacgaatcg ccgcgaaaca gcgtcgccgg ccgacgcagc
     2761 ggcggatcct tgggcgaggg cagggcggcg caccgcgacg gtctgtcgcg cccgcggacg
     2821 ccgaccggga cggcgtgtcg cggcgctggc tggtggtcgc gtacccggct acggtccctc
     2881 cgcgggtccc tggcgacggc ttggcgaggt gccagcggtc acgggcccgg gcggcaaaac
     2941 ctcgctttac cgccgctcac gactgttgcg cacctggcca cagtgctgtt agggtgcgcg
     3001 ccgaaacaag ggtcctggac gtatttcgtc gctttgttca aagcccccga gatccgcaca
     3061 gcgcgagggt cggcgagcct cgctc
//
LOCUS       DASWNJ010001327         2090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_235432, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001327 DASWNJ010000000
VERSION     DASWNJ010001327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235432"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            382..>2090
                     /locus_tag="VGB85_22125"
     CDS             382..>2090
                     /locus_tag="VGB85_22125"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HEY0136803.1"
                     /translation="MTTAVIAEKPSVARDIAAALGATRRGEGCFEGAGYVVTWAIGHL
                     VALAQPHEIDPAWKRWSLATLPMLPRSFPLVVVPGTRDQFAVVKRILGDRSVTQVICA
                     TDAGREGELIFRYIYEAASCKKPVRRLWISSLTREAILDGLSRLRSGAEYDGLADAAR
                     GRSRADWLVGMNLSRAYSLVFDQDLSVGRVQTPTLAILVERELAIRDFVPEDYREVVA
                     RFQPIAEPAPLAYVGTWFRPPGPRDKEDGDPGKRKRLPADGVEAAAIVARALTGQAAI
                     ASTKAETKKIPPPLLYDLTELQRHANRLYGMSAQKTLDVAQALYERHKLISYPRTDSR
                     HLSSDIAATLGAVVAAVGGRYPGLLAPGTGTTPLGRRFVDDTRVSDHHAILPTSARAG
                     ALAADEQKIYDLICRRFLEAWHEDHVYSATTVITTITSAGPDQPAIVDHYESRGTAIQ
                     NVGWKVLEVGHGKPPAKPRSKPADSTSEPDPGEQALPAGLVRGQAQRVLEAKAIAKQT
                     RPPPRHSEGTLLTAMETAGKALEEKELSEAMRDLGLGTPATRAQIIETLLRREYVVRD
                     GKSL"
BASE COUNT          310 a          775 c          699 g          306 t
ORIGIN      
        1 gagtgccatc gtccaccggc tcgcactgtc cctcttctgt gaacgtccgg cgccccgccc
       61 cccggtcgcg ttgccgtccg aaccgggttc ggcgagttgg ctgcctgatg atccctgggc
      121 ctgatgccgc ggccgtccaa cgagagcttc acgtcgcgct tctgcaagtc ctcgccgctc
      181 tccccgaagg cgactgacac tctggactca agacgccggg ccttctcggt tgccccagtc
      241 caagaaattc tgtgcatgat ccttcgccgc tcgggcctgt tcgtgccgcc gccctcgccc
      301 gcctgcgcgg ctgctgcggc gcagatccgc atgtgctaag gtcccgcgct cgctggcgtc
      361 gccgctggcg ccaccgtcgc cgtgaccacc gccgtcatcg ccgagaagcc ctccgtggcc
      421 cgcgacatcg ccgcggcgct cggcgcgacg cggcgcggcg agggctgctt cgagggggcg
      481 ggttacgtcg tgacctgggc gatcggccac ctcgtcgcgc tcgcccagcc gcacgagatc
      541 gatcccgcgt ggaagcgctg gagcctggcg accctgccga tgttgccgcg cagcttcccg
      601 ctggtcgtcg tgcccggcac gcgcgaccag ttcgcggtgg tgaagcggat cctcggcgat
      661 cgctcggtca cgcaggtcat ctgcgcgacc gatgccggcc gcgagggcga gctgatcttt
      721 cgctatattt atgaggccgc cagctgcaaa aagccggtcc gccggctctg gatctcctcg
      781 ttgacgcgag aggcgatcct cgacggcctg tcgcggctgc gcagcggcgc cgaatacgac
      841 ggcctcgccg acgccgcgcg cggtcgcagc cgcgccgact ggctggtcgg catgaacctg
      901 tcgcgcgcat acagcctggt cttcgatcag gacctctcgg tcggtcgggt ccagacgccg
      961 acgctggcga tcctcgtcga gcgcgagctg gcgatccgcg acttcgtgcc cgaggattat
     1021 cgcgaggtcg tcgcccgctt ccagccgatc gccgagccgg cgccgctcgc ctatgtgggc
     1081 acatggtttc ggccgccggg tccgcgcgac aaggaggacg gggatcccgg gaaacgcaag
     1141 cggctgccgg cggacggagt ggaggccgcg gcgatcgtcg cccgcgcgct caccggccag
     1201 gcagcgatcg cgtcgacgaa ggccgagacg aagaagatcc cgccgccgct gttgtatgac
     1261 ctcaccgagc tgcagcgcca cgccaatcgc ctgtacggca tgagcgcgca gaagaccctc
     1321 gatgtcgccc aggcgctgta cgaacgccac aagctgatca gttatcctcg caccgacagt
     1381 cgccacctct cgagcgacat cgccgcgacg ctcggcgccg tggtcgcggc cgtcggcggt
     1441 cgttaccccg gcctgctcgc gcccggcacc ggcacgacgc cgctcggccg gcgcttcgtc
     1501 gacgataccc gggtcagcga ccaccacgcg atcttgccga cctcggcccg cgcgggcgcg
     1561 ctcgcggccg acgagcagaa aatttacgat ctgatctgcc ggcgcttcct cgaggcatgg
     1621 cacgaggacc acgtctattc ggcgacgacc gtgatcacca cgatcacctc cgccggaccc
     1681 gaccagccgg cgatcgtcga tcactacgag agccgtggca ccgcgatcca gaacgtcggc
     1741 tggaaggtgc tcgaggtcgg ccacggcaaa ccgcccgcga agccgcgcag caaaccggcc
     1801 gactccacga gcgagccgga ccccggcgag caggccctgc ccgccggcct cgtgcgtggc
     1861 caggcgcagc gggtgctcga ggcgaaggcg atcgcgaagc agacgcggcc gccgccgcgc
     1921 cacagcgagg gcaccctgct gaccgcgatg gagaccgccg gcaaggcatt ggaggagaag
     1981 gagctgtcgg aggcgatgcg cgacctcggc ctcggcaccc cggcgacgcg cgcccagatc
     2041 atcgagaccc tcctgcgccg cgaatatgtc gtccgcgacg gcaagtcgct
//
LOCUS       DASWNJ010001328         1916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_307056, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001328 DASWNJ010000000
VERSION     DASWNJ010001328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307056"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /locus_tag="VGB85_22130"
     CDS             complement(<1..1327)
                     /locus_tag="VGB85_22130"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0136804.1"
                     /translation="MEPRTTRTRRRGVAPIHDPQPGTTLGGRYTIVRLIARGGVGLVY
                     LARRSISNDAVVVKVLAASVVGDSETAARFDREAQRLRGIQHPNIVAMVDYGRTDGSA
                     YLVMEYLEGELLSAYAARRGPLALADFTPIAAQILKAIGYAHEHGLMHRDLKPSNIML
                     CTRKGRANFVKILDFGMAKLVDDERDITSEQIVGTANYLSPEQIRGEAIDARVDVYAI
                     GVMFYALLSGRLPFTADNNAALLYKHVHEPPPPLGSRLPDGHDIPPQLVALVHRCLAK
                     DVADRPLDANAVAAELVACIPGELFHLPVATSGAATASSSYAVLPASIAAGPDDLSDR
                     LTRPVQSLRAARTGQAATTDDVEAAATAPITGATAATGRARTGNRLPRMRPATLPPSI
                     PIMPIVMTPPPGEGGKLWLVLAGVVVLALLGLGGWLVSHAGRTADASQGP"
     gene            complement(1405..>1916)
                     /locus_tag="VGB85_22135"
     CDS             complement(1405..>1916)
                     /locus_tag="VGB85_22135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136805.1"
                     /translation="LIANLEGVAPDVLDPDNFASFNVRYKLRFNDTDAITFSSLAYDA
                     ALVTIFAMSAIPQGEPVTGAAIAGNIGKLVGGNGPKVSFGDVDGTDLLFIKKAHNALV
                     SGGTVDLKGVSGELNFDLTTGEVRTNVIGWGLEPKDMMNPTVPVLAPARVYVLDPVPS
                     EVGTWMNLP"
BASE COUNT          252 a          611 c          767 g          286 t
ORIGIN      
        1 cggggccctg cgaggcgtcg gcggtgcgcc cggcgtggga cacgagccag ccgccgaggc
       61 cgagcagcgc gagcacgacg acgcccgcga gcaccagcca cagcttgccg ccctcgcccg
      121 ggggcggggt catgacgatc ggcatgatgg ggatactcgg cggcagcgtg gccgggcgca
      181 tccggggcag gcggttgccg gtgcgggcgc ggccggtcgc ggcggtcgcc ccggtgatcg
      241 gcgccgtcgc ggcggcctcg acgtcgtcgg tcgtcgcggc ctggccggtc cgggcggccc
      301 gcagcgactg cacgggtcgc gtcaggcggt cgctgaggtc gtcggggccg gcggcgatgc
      361 tggccggcag caccgcgtac gacgacgagg ccgtcgccgc gccgctggtg gcgaccggca
      421 ggtggaacag ctcgccgggg atgcaggcga ccagctcggc ggcgacggcg ttggcgtcga
      481 gcggccggtc ggcgacgtcc ttggcgaggc agcgatgcac gagcgcgacc agctgcggcg
      541 ggatgtcgtg gccgtccggc aggcgggagc ccagcggcgg cggcggctcg tggacgtgct
      601 tgtagagcag cgcggcgttg ttgtcggcgg tgaacggcag gcgcccggag agcagcgcgt
      661 agaacatgac gccgatcgcg tagacgtcga cgcgggcgtc gatcgcctcg ccgcggatct
      721 gctccggcga gaggtagttg gcggtgccga cgatctgctc ggaggtgatg tcgcgctcgt
      781 cgtcgacgag cttcgccatg ccaaagtcga ggatcttgac gaagttggcg cgccccttgc
      841 gggtgcacag catgatgttg ctcggcttga ggtcgcggtg catcaggccg tgctcgtgcg
      901 cgtagccgat cgccttgagg atctgcgcgg cgatcggcgt gaagtcggcg agcgccagcg
      961 ggccccttcg cgccgcgtag gcgctgagca gctcgccctc gaggtactcc atcaccaggt
     1021 aggcggagcc atcggtgcgg ccgtagtcga ccatcgccac gatgttgggg tgctggatcc
     1081 cgcgcaggcg ctgggcttcg cgatcgaagc gcgccgcggt ctcgctgtcg ccgacgacgc
     1141 tggccgcgag caccttgacg accacggcgt cgttgctgat cgaccggcgc gcgagataga
     1201 ccaggccgac gcccccgcgc gcgatcaggc ggacgatcgt gtagcgaccg ccgagcgtgg
     1261 tgccgggctg cgggtcgtgg atcggcgcga cgccgcggcg gcgcgtccgg gtggtgcgag
     1321 gctccacgct gcgagcttag cgcaggccct caccggctgt ctgcacgctc gacgcgatcg
     1381 cgccggggcc ggtaaatcag tgttctatgg gagattcatc cacgtgccga cctcggaggg
     1441 caccgggtcg agcacgtaga cgcgggccgg ggccaggacc ggcacggtcg ggttcatcat
     1501 gtccttgggc tcgaggcccc agccgatgac gttggtgcgg acctcgccgg tggtcaggtc
     1561 gaagttgagc tcgccgctga cgcccttgag gtcgacggtg ccgccgctga cgagcgcgtt
     1621 gtgggccttc ttgatgaaca ggaggtcggt gccgtcgacg tcgccgaagc tgaccttggg
     1681 gccgttgccg ccgacgagct tgccgatgtt gccggcgatc gcggcgccgg tgaccggctc
     1741 gccctggggg atcgccgaca tcgcgaagat cgtcaccagc gcggcgtcgt aggccagcga
     1801 ggagaaggtg atcgcgtcgg tgtcgttgaa gcgcagcttg tagcggacgt tgaagctggc
     1861 gaagttgtcg gggtcgagca cgtccggggc gacgccctcg aggttggcga tcagcg
//
LOCUS       DASWNJ010001329         1669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_24722, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001329 DASWNJ010000000
VERSION     DASWNJ010001329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24722"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VGB85_22140"
     CDS             complement(<1..436)
                     /locus_tag="VGB85_22140"
                     /inference="COORDINATES: protein motif:HMM:NF015884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HEY0136806.1"
                     /translation="MTKFKLEYIWLDGHTPVPNLRGKTQIKEFGEFPSLEELPLWGFD
                     GSSTMQAEGRSSDCVLKPVRVFPDSERKNGALVLCEVMMPDGETPHPSNKRATILDDE
                     GAWFGFEQEYFFYKNGRPLGFPESGYPAPQGPYYTGVGYSQVG"
     gene            complement(527..865)
                     /locus_tag="VGB85_22145"
     CDS             complement(527..865)
                     /locus_tag="VGB85_22145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY0136807.1"
                     /translation="MRKIEAVIKPFKLDEVKEALQEIGLKGMTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEIVVEDGVVERAVEAIQAAARTDRIGDGKIFIIPVEDAIRI
                     RTGERGTDAL"
     gene            1156..1232
                     /locus_tag="VGB85_22150"
     tRNA            1156..1232
                     /locus_tag="VGB85_22150"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1190..1192,aa:His,seq:gtg)
     gene            complement(1265..>1669)
                     /locus_tag="VGB85_22155"
     CDS             complement(1265..>1669)
                     /locus_tag="VGB85_22155"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0136808.1"
                     /translation="AHTTFAMCYVAVIVQSRLAGFDRSLEEAAMDLGAPPLRTFMLVT
                     LPLIAPALVAGWLLAFTLSLDDLVIASFTTGPGATTLPMRIYSQVRLGVTPEINAVST
                     VLVGLVTVAVVAASLAQRRRLARDMAGTTNAR"
BASE COUNT          251 a          583 c          545 g          290 t
ORIGIN      
        1 cgccgacttg gctgtagccg acgcccgtgt agtaggggcc ttggggtgcc gggtagccgg
       61 actcggggaa gcctagcggc cggccgttct tgtagaagaa atactcctgc tcgaagccga
      121 accaggcgcc ctcgtcgtcg aggatggtcg cccgcttgtt cgacgggtgc ggcgtctcgc
      181 cgtcgggcat catgacttcg cacaggacga gcgcgccgtt cttgcgctcg ctgtccggaa
      241 acacccggac cggcttcagg acgcagtcgg aactccgccc ctccgcctgc atggtggagc
      301 ttccgtcgaa gccccacaac ggcagctcct cgagcgacgg gaactcgccg aattccttga
      361 tctgcgtctt gccgcgcagg ttggggacgg gcgtgtgccc gtcgagccag atgtactcga
      421 gcttgaactt tgtcactgcc gccctcttgc aagctgttgg cccgggacaa gaagtcgtgt
      481 cgcgtgcacg acgcccggcc gccctgtcgt cctgccgccg atggtgctag agcgcgtccg
      541 tgccccgctc gcccgtccgg atgcggatgg cgtcctcgac cgggatgatg aagatcttgc
      601 cgtcgccgat ccggtcggtc ctggccgcgg cctggatcgc ctcgaccgcg cgctcgacca
      661 cgccgtcctc gaccacgatc tcgatcttca ccttcggcag gaagtcgacc acgtactcgg
      721 cgccgcggta cagctccgtg tgccccttct gccgcccgaa gcccttcacc tcggtcacgg
      781 tcatgccctt caggccgatc tcctgcaggg cctccttcac ctcgtcgagc ttgaacggct
      841 tgatgaccgc ctcgatcttc ctcatgcgcg cctggtctcc cgtagcgttc ccgcgaatcg
      901 cggtgcgccc gctccttggt tcccgttctt agcagggagc gtgccagcgg tgccgcgatg
      961 ccgtcgcgga cggcaatcgg acgacctctg ccgccgcttt caccacagcg gttgcgcctg
     1021 cgcgaggcag aaagccagaa atttaggcaa ctcgggatgc tcgccgacgc gcaggacggg
     1081 actaggcttt gcgccggggc tagcggcccg ccttgacgac gcgagcccgt tgcgccaaat
     1141 caggcgccga tcatggcggg tgtagctcag taggttagag caccaggttg tggccctggg
     1201 ggtcgccggt tcaagtccgg tcactcgccc caaatccctt cgctcgatta gccgtcgtcc
     1261 cacctcagcg ggcgttcgtg gtcccggcca tgtcgcgcgc cagccggcgc cgctgcgcca
     1321 gcgacgccgc caccacggcc accgtcacca gcccgaccag caccgtcgag acggcattga
     1381 tctccggcgt aacacctaag cgcacctggc tgtagatccg catcggcagg gttgtagcgc
     1441 cgggcccggt ggtgaagctc gcgatgacga ggtcgtcgag cgagagggtg aaggcgagca
     1501 gccagcccgc cacgagggcc ggcgctatca gcggcagggt gaccagcatg aaggtgcgca
     1561 gcggcggtgc gcccaggtcc atcgccgcct cctcgagcga gcggtcgaaa ccggcgaggc
     1621 gcgactggac gatcacggcc acgtagcaca tggcgaaggt cgtgtgcgc
//
LOCUS       DASWNJ010001330         7732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_694085, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001330 DASWNJ010000000
VERSION     DASWNJ010001330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7732
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694085"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..735
                     /locus_tag="VGB85_22160"
     CDS             133..735
                     /locus_tag="VGB85_22160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136809.1"
                     /translation="MSRLSLCLSLLCTLSLACGDGSNDETAGSSSSGATSDTPTGEPI
                     ALDCNTYCTTITSNCTAQNAQYASMAQCETSCGAFALGTDADQMGNTLGCRIYHAGAA
                     AGAPETHCTHAGPGGNNQCGSNCEGFCALASFACPGSFSDGEACMSACAGYPDSEPFD
                     AADVGGDTLACRLYHLTVASEAPTVHCPHILPDSEPCGGA"
     gene            802..1707
                     /locus_tag="VGB85_22165"
     CDS             802..1707
                     /locus_tag="VGB85_22165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136810.1"
                     /translation="MPTSVGVLRLAPLALLLCGCFTDPGRYGASEGSSTGAGEATADP
                     GTTAPPTTDDTLTTTLPGHATTSSSDPTGEPPDGTTTTGPPDPTSTTTSTTEPATTGP
                     PPAQCGNGKIDPGEACDDGDFNGGAGCTFDCSVPSCFDLLLNQGESDLDCGGPCQPCA
                     ACRACNDDTDCVVPTPCAGGVCAGATPITINYLLNCAENVDDWPLGPVFPAGEYRITA
                     VGGGGAVKEGPDYYGWLVQCDGLDITEMSVPFVYASPQDAFQALPTKKIVRAYPGGPL
                     RCGLQDIACEDNSGAVSLSFAPECL"
     gene            complement(1739..3967)
                     /locus_tag="VGB85_22170"
     CDS             complement(1739..3967)
                     /locus_tag="VGB85_22170"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0136811.1"
                     /translation="MNAPSLAKWILLATPLMFSGCRTSNSVTPIGGTIEPGRDGGDGN
                     GALLVQGQGSYDAPPHLFSPRPHPPRARMDPPAPDLNALPKLVNRIDRCYGSERPADL
                     STPSPRPRPMPRPTSGHKGKYKQVTYGGATTTPSTPAPANGGMIAGGTSTSSKSASGR
                     ASSAGDAKPKDGSARPAPKKRAESAYEGAPSVAPSAPAGASAAPSANASPQEDALTSR
                     DRRAERKEKRDERRNHKAGGDSASAPQEIAADDDEEAEEAEARPVQLDDPRDQYKDWG
                     SAIYLSNDDTMSLSSAQRVIFAIEKFLPLPVEHIRPHELLNYFSFETAPVAPQDDFSV
                     KADISPDAKQQGIYNLALAIRGRPVDMQSRRNANLTFVVDRSGSMSDEGRMDYLKRGM
                     TRMTSQLKRGDLINVVLFDHEECTPIENFVVGRDRPEILQKAIDKMQPRGSTDVNLGL
                     TKGYALADAAHQPTYNNRVVLVTDALANTGDTDPRTMAMVAKYYDSRKIRLSGVGVGR
                     EFNDALLDKMTERGKGAYVFLGSEAEVDAVFGARFISLLETTAMDVHFQLHLPPSLRM
                     NVFYGEESSVVKEDVQAIHYFANTSQLFLSDLMARGGKLRPQDQIMLTIEYEDPESGT
                     KMLEERAFTLGEVAQEQSFNVSKGRLLIGWADRLAALATRPQPYGHNAMAGSWDDPEG
                     WQTCDTGRAELGNLAQGLNDPEAARVLGLWDKFCARYEQPRSPVRRTPAASPSAWPGA
                     SG"
     gene            4656..5831
                     /locus_tag="VGB85_22175"
     CDS             4656..5831
                     /locus_tag="VGB85_22175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136812.1"
                     /translation="MESLLLVVAELVSVPRLVALTLLVALAAAVALILGHLTIGRRLH
                     SWPLRWWRRLVWSTIGGLGFMLAGLVFIDLLLFAPALRIALDQIELRSGLDVGFRSAD
                     GSLLTGRLELHDVTLRRSSTGLDLSLTVQHLSIDVDMLRLYRAELPIDLVRADGVRGT
                     IVRRDGDTPGPARPFIIDRLELEDVQVDFEDAITAPFRSLPIVFTSLKLSPLRSEFAL
                     LDALCGSEASGSARGYAFGAGGHAWQARGIPIGPAAHKLGAAGRWIRGGDIDLTLRCD
                     PGRDTEAVPLAITLHLHDLKIAPPGDSGRHPMASQIAGALARLGSEATMDFAFELPRE
                     RFRGASNVGQLGLWEGAILGWNLALAARLGLTQEQLRVFGLGSRVLDKLAPEPARRN"
     gene            5863..6615
                     /locus_tag="VGB85_22180"
     CDS             5863..6615
                     /locus_tag="VGB85_22180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136813.1"
                     /translation="MLGLRFDRLVHAALAGGGLTLSACPGGGQDTDTDTDTDGTDSDP
                     TTSVEPTTGPPPPPPPPADITPPALVAVEFLDPQILRLSFTEAIASVDEVNPKRFRLS
                     IGRFYENAYYGYSRTLYNDPDDFNHNLYCAEECYYGSCYEQCYYGPTLEVDILDLLTD
                     AYDPAKIVLLLEQPVTPSLCQLVTNISGSGTGGLLIHYADGGASQITDTAGLPLPGTG
                     ATWVKIANQNYYTIDNQKFPEQNPFLPIPCPF"
     gene            6752..>7732
                     /locus_tag="VGB85_22185"
     CDS             6752..>7732
                     /locus_tag="VGB85_22185"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0136814.1"
                     /translation="MPPRTAVPWDATVAEHLAAASGALASAGERPPQEWIGRVIDERY
                     RIVEILGEGGMGAVFVAEHLKLRKQVALKTIRPEFASNSQAEARFAREALATAQIDHP
                     HVASAIDFGHLPEGGAYLVIQLVRGVSLAAHLDTHGRIAWATAATLGAQIADGLAAAH
                     AAGIVHRDLKPDNILIETRDDGRLHAKVLDFGIAHVAEVTSADASGASANSLSQPLTR
                     MGAILGTPGYMPPEQAVGEAVDARGDLYALGVILWECCTGRRLWQGESVIELVTCQLG
                     STPPTLQQELGSQVPEALSELVASLLAASPSKRPEAAGPVRDALRRLSLKE"
BASE COUNT         1041 a         2972 c         2582 g         1137 t
ORIGIN      
        1 cgttgccggc cggcgcccgc gagtgcgtcg cgcaggccgg gtccgccgag cagatcagcc
       61 acgagtgcct gcactgatcg cgcgcacgaa accctttgac cgccgcggtg cgccgcgcca
      121 atagtgtcgg gcatgtcgcg cctgtccctg tgtctctccc tgctgtgcac cctctcgctc
      181 gcctgcggcg acggctcgaa cgacgagacc gcgggctcga gcagcagcgg ggccaccagc
      241 gacacgccga ccggcgagcc gatcgcgctc gactgcaaca cctactgcac cacgatcacc
      301 agcaactgca ccgcgcaaaa cgcccagtac gcgagcatgg cgcaatgtga gacctcgtgc
      361 ggcgccttcg cgctcggcac cgatgccgac cagatgggca acacgctcgg ctgtcgcatc
      421 taccacgccg gcgccgccgc gggcgcgccg gagacccact gcacgcacgc gggccccggc
      481 ggcaacaatc aatgcggcag caactgcgag ggcttctgcg cgctcgcctc gttcgcgtgc
      541 ccgggcagct tctccgacgg cgaggcctgc atgagcgcct gcgccggtta ccccgacagc
      601 gagcccttcg acgccgcgga cgtcggcggt gacaccctcg cgtgtcgcct ctaccacctg
      661 acggtcgcca gcgaggcgcc gaccgtgcac tgcccgcaca tcctccccga ctcggagccg
      721 tgcggcggcg cctgatcggc gtgctcgcgc gaggctgcct cgccttgaca accggccccg
      781 ccgcttcgat cgtggttggc catgcccacg tccgtcggcg tcctgcgcct cgctccgctc
      841 gcgctcctgc tgtgcggctg cttcaccgac cccgggcgct acggcgcgag tgagggcagc
      901 tcgacgggcg ccggcgaggc cacggccgat ccaggcacga cggcgccgcc gaccaccgat
      961 gacaccctga ccaccacgct gcccgggcac gccaccacct catcgtcgga cccgacgggc
     1021 gagccgcccg acggcaccac gaccaccggc ccgcccgacc ccacttcgac cacgacctcg
     1081 accaccgagc cggcgaccac cggcccgccg ccggcgcagt gcggcaacgg caagatcgat
     1141 cccggcgagg cctgcgacga cggcgacttc aacggcggcg cgggctgcac tttcgactgc
     1201 agcgtcccga gctgcttcga cctcctgctc aatcagggcg agtccgacct cgactgtggc
     1261 ggcccgtgtc agccgtgcgc ggcctgccgc gcgtgcaacg acgacaccga ctgtgtcgtg
     1321 ccgaccccgt gcgccggcgg cgtgtgtgcc ggcgcgacgc cgatcacgat caactacctg
     1381 ctcaactgcg ccgagaacgt cgacgactgg ccgctcgggc cggtcttccc cgcgggcgag
     1441 taccggatca ccgccgtcgg cggcggcggg gcggtcaagg aagggcccga ctactacggc
     1501 tggctggtcc agtgcgacgg cctcgacatc accgagatga gcgtgccctt cgtctacgcg
     1561 agcccgcagg acgccttcca ggccctgccg accaagaaga tcgtgcgtgc gtaccccggc
     1621 ggccccctgc gctgcggcct gcaggacatc gcctgcgagg acaactccgg cgccgtcagc
     1681 ctgtcgttcg cgcccgagtg cctgtgaagg gccaggcgcg cctggccccg gggacatgtc
     1741 agccgctcgc gcccggccac gcgctcggcg acgccgccgg tgtccgccgc accgggctgc
     1801 gcggctgctc gtagcgcgcg cagaacttgt cccacaggcc gagcacgcgc gccgcctccg
     1861 ggtcgttgag gccctgggcc aggttaccga gctcggcgcg gccggtgtcg caggtctgcc
     1921 agccctcggg gtcgtcccag ctgccggcca tcgcgttgtg cccgtacggc tgcgggcggg
     1981 tcgccagcgc cgcgaggcgg tcggcccagc cgatcaacag ccggcccttg ctgacgttga
     2041 acgactgctc ctgcgcgacc tcgccgagcg tgaacgcccg ctcctcgagc atcttggtgc
     2101 cgctctccgg atcctcgtac tcgatcgtca gcatgatctg gtcctgcggg cgcagcttgc
     2161 cgccgcgggc catcaggtcg gacaggaaca gctgcgaggt gttggcgaag tagtggatcg
     2221 cctgcacgtc ctccttcacc accgagctct cctcgccgta gaacacgttc atgcgcagcg
     2281 acggcggcag gtgcagctgg aagtgcacgt ccatcgccgt cgtctcgagc agcgagatga
     2341 agcgcgcccc gaacaccgcg tcgacctcgg cctcggagcc gaggaacacg tacgccccct
     2401 tgccgcgctc ggtcatcttg tcgagaagcg cgtcgttgaa ctcgcgcccg accccgaccc
     2461 ccgagagccg gatcttgcgc gaatcgtagt acttcgccac catcgccatc gtccgcggat
     2521 ccgtgtcgcc ggtgttggcc agcgcgtcgg tcaccagcac cacgcggttg ttgtaggtcg
     2581 gctgatgcgc cgcgtcggcc agggcatagc ccttcgtcag ccccaggttc acgtcggtcg
     2641 acccgcgcgg ctgcatcttg tcgatcgcct tttgcaggat ctccggccgg tcacggccga
     2701 ccacgaagtt ctcgatcggc gtgcactcct cgtggtcgaa cagcaccacg ttgatcaggt
     2761 cgccgcgctt gagctgcgag gtcatcctcg tcatgccgcg cttgaggtag tccatgcggc
     2821 cctcgtcgga catcgagccc gagcggtcga ccacgaaggt caggttggcg ttgcgccgcg
     2881 actgcatgtc gaccgggcgg ccccggatcg ccagcgcgag gttgtagatc ccctgctgct
     2941 tcgcgtcggg cgagatgtcc gccttgaccg agaagtcgtc ctgcggcgcg accggggccg
     3001 tctcgaagct gaagtaattg agcagctcgt gcggccggat gtgctcgacc ggcagcggca
     3061 ggaacttctc gatagcgaag atcacccgct gcgccgacga caggctcatc gtgtcgtcgt
     3121 tcgagaggta gatcgccgag ccccagtcct tgtactggtc gcgcgggtca tcgagctgca
     3181 ccggccgcgc ctcggcctcc tcggcctcct cgtcatcgtc ggccgcgatc tcctgcggcg
     3241 ccgaggccga gtccccgccg gccttgtgat tacgccgctc gtcgcgcttc tccttgcgct
     3301 ccgcccgacg gtcacgcgaa gtcaacgcgt cctcctgcgg cgacgcgttc gccgagggcg
     3361 cggccgatgc cccagccggc gccgacgggg ccaccgacgg ggcgccctcg tacgcggatt
     3421 cggccctctt cttcggcgcc ggcctggccg agccgtcctt cggcttcgcg tcgccggccg
     3481 agctcgcgcg accgctcgcg ctcttgctcg aggtcgacgt cccccccgcg atcatgcccc
     3541 cattcgccgg cgctggcgtc gacggcgtcg tcgtcgcgcc gccgtaggtc acctgcttgt
     3601 acttgccctt gtgcccgctg gtcggccgcg gcatcggtcg cggtcgcggc gatggcgtcg
     3661 aaaggtcggc cgggcgctcg ctgccgtagc agcggtcgat gcgattgacg agcttcggca
     3721 gcgcgttgag gtccggcgcc ggtgggtcca tacgcgcgcg cggcggatgc gggcgcggtg
     3781 agaacaggtg cggcggcgcg tcgtagctgc cctgcccctg gaccagcagc gcgccgttgc
     3841 cgtcgccgcc gtcacgaccg ggctcgatcg tgccgccgat cggcgtgacg ctgttcgagg
     3901 tgcgacagcc ggagaacatc agcggcgtgg cgagaaggat ccacttggcg agagacgggg
     3961 cgttcatcgg gcactcctgc aagatcggac ttcgcgaggc aacaccgccg cacgaccggc
     4021 catcccggcc gtcgcgcacc ggagcctccg ggcgccgatg gaacgacgcc gaccacctcg
     4081 agctttcgcc tccccgacaa aattgtccac gccgccagcc tcgccctgtt aacgccgttc
     4141 gtcggccccg gcgagcgacc accgacgcga tgcgtcgcgc tgcgcggccg ggcatcgccg
     4201 cacgaggccc ggatcgccgg cgggatcgcc cgtgtgggtg gcgtcgccgc ggtgcccgtc
     4261 gcggggtcgc tcgcgcccgt atcgctcgcg gcgctcatgc caccgccggt cgcctcggtc
     4321 gtcgtcgcgc cgctcgtcgg cggccccgcc ggaccggtcc tgccttcacc tgtcccgtgg
     4381 gacatgctcg aaaagccacc ccggtcgcgt ccccggtcgc cgtgagcccc gcgtcggccc
     4441 tcaccgcggt cgcacgggcg cgccggaggt ggcgggcgcc gatcggcgag gcgcgacgcg
     4501 ccccaccgag ctcggggcgc gggcgtgtgg cgatcgtcgc cggtgtaggt ccgatactgc
     4561 gcgcatgtca aggcgagcgg ctgccctcta tcgagcccga cggccggggg cgccgcggct
     4621 tgttccggcg ggcggcgcgg tgcgacactg gcagcgtgga gtcgctgctg ctcgtcgtcg
     4681 ccgagctggt gtcggtgccg cgtctcgtgg cgctcacgtt gctcgtggcg ctcgccgccg
     4741 ccgtcgcgct gatcctcggg cacctgacga tcgggcggcg gctgcactcg tggccgctgc
     4801 ggtggtggcg acggctggtg tggagcacga tcggcggcct cggcttcatg ctcgccggcc
     4861 tggtgttcat cgacctgctg ctgttcgcgc cggccctgcg gatcgcgctc gatcagatcg
     4921 agctgcgctc gggcctcgac gtcgggtttc gcagcgccga cggcagcctg ttgaccggcc
     4981 gcctcgagct gcacgacgtc accttgcgcc gcagcagcac cggcctcgac ctctcgctga
     5041 cggtgcagca cctgagcatc gacgtcgaca tgttgcggct gtatcgcgcc gagctgccga
     5101 tcgacctcgt gcgcgcggac ggggtccgcg ggacgatcgt ccgccgcgac ggcgacaccc
     5161 cgggcccggc gcggcccttc atcatcgacc gcctcgagct cgaggacgtg caggtcgact
     5221 tcgaggacgc gatcacggcc ccgtttcgca gtcttccgat cgtcttcacc agcctcaagc
     5281 tcagcccgct gcgcagcgag ttcgccctgc tcgacgcgct gtgcggcagc gaggccagcg
     5341 gcagcgcccg cggctacgcc ttcggggccg gcggtcacgc ctggcaggcg cgcgggatcc
     5401 cgatcggccc cgcggcgcac aagctcgggg ccgccggccg atggatccgc ggcggtgaca
     5461 tcgatctgac gctgcgctgc gaccccggtc gtgacaccga ggccgtgccg ctggcgatca
     5521 cgctgcacct gcacgacctc aagatcgcgc ctcccgggga cagcggccgc cacccgatgg
     5581 ccagccagat cgccggcgcg ctggcccgcc tcggctccga ggcgaccatg gacttcgcct
     5641 tcgagctgcc gcgcgaacgc ttccgtggcg ccagtaacgt cggtcagctc gggctgtggg
     5701 agggggccat cctcggctgg aacctcgcgc tcgcggccag gctgggcctg acccaggagc
     5761 agctgcgggt gttcgggctc ggctcgcgcg tgctcgacaa gctggccccc gagcctgcgc
     5821 gccgcaattg accgcccgcc gggcatttgc aaggatcgcc ggatgcttgg cctacgtttc
     5881 gaccgcctcg tccacgccgc gctcgcgggc ggaggcttga ccctcagcgc ctgtccgggc
     5941 ggcggtcagg acaccgacac cgacaccgac accgatggca ccgacagcga cccgacgacg
     6001 agcgtcgagc cgaccaccgg accgccgccg ccaccgccgc cgccggccga catcacgccg
     6061 cccgcgctgg tcgccgtcga gttcctcgac ccacagatcc tccgcctcag cttcaccgag
     6121 gcgatcgcgt cggtcgacga ggtcaacccc aagcgctttc gcctcagcat cggtcgcttc
     6181 tacgagaacg cctactacgg ctacagccgc accctctaca acgaccccga cgacttcaac
     6241 cataacctct actgcgccga ggagtgctac tacggcagct gctacgagca gtgttactac
     6301 ggcccgacgc tcgaggtcga catcctcgat ctgctgaccg acgcgtacga ccccgcgaag
     6361 atcgtcctgc tcctcgagca gccggtgacg ccgagcctgt gccagctggt gaccaacatc
     6421 agcggctccg ggaccggcgg cctgctgatc cactacgccg acggcggcgc ctcccagatc
     6481 accgacaccg cgggcctgcc gctgcccggc accggcgcga cctgggtgaa gatcgccaac
     6541 cagaactact acacgatcga caaccagaag ttccccgagc aaaacccgtt tttgccgatc
     6601 ccctgcccgt tctagcccgt tcatcgcaac ctgccggggt cgcgcgcggc cctgacggcc
     6661 gcgcatcgcc gcccgtcgcg cgcgagcgac cgcccgggca tggcttttcg gacaggctcg
     6721 ccgcgccggg ccgcgggtat actccgccgc catgcccccc cgcaccgcgg tcccgtggga
     6781 cgccacggtc gccgagcacc tcgctgcggc ctcgggcgcg ctcgcgtcgg cgggcgagcg
     6841 acccccgcag gagtggatcg ggcgcgtcat cgacgagcgc taccgcatcg tcgagatcct
     6901 cggtgagggc ggcatggggg ccgtgttcgt cgccgagcac ctcaagctgc gcaagcaggt
     6961 cgcgctcaag acgatccgcc cggagttcgc cagcaactcg caggccgagg cccgcttcgc
     7021 ccgcgaggcc ctcgccaccg cgcagatcga tcacccgcac gtcgccagcg cgatcgactt
     7081 cgggcacctg ccggagggcg gcgcctacct cgtcattcag ctggtccgcg gggtcagcct
     7141 cgccgcgcac ctcgacaccc acggtcggat cgcctgggcg accgccgcga ccctcggggc
     7201 ccagatcgcc gacggcctcg ccgctgcgca cgccgccggc atcgtgcatc gcgacctcaa
     7261 gcccgacaac atcctgatcg agacccgcga cgacggccgc ctgcacgcca aggtcctcga
     7321 cttcggcatc gcccacgtcg ccgaggtgac ctccgcggac gccagcggtg cgagcgcgaa
     7381 cagcttgagc cagccgctga cgcgcatggg cgcgatcctc ggcacgcccg gctacatgcc
     7441 gcccgagcag gccgtcggcg aggccgtcga cgcgcgcggc gatctctacg cgctcggcgt
     7501 catcctctgg gagtgctgca ccggccgtcg tctgtggcag ggcgagagcg tgatcgagct
     7561 cgtcacctgc cagctcggct cgaccccgcc gaccctgcaa caggagctcg gcagccaggt
     7621 gcccgaggcg ctcagcgagc tcgtcgccag cctgctcgcc gcgtcgccga gcaagcgccc
     7681 cgaggccgcc gggcccgtgc gcgacgctct gcgccgcctg tcgctcaagg ag
//
LOCUS       DASWNJ010001331         1603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_24786, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001331 DASWNJ010000000
VERSION     DASWNJ010001331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24786"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..340)
                     /locus_tag="VGB85_22190"
     CDS             complement(50..340)
                     /locus_tag="VGB85_22190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY0136815.1"
                     /translation="MIGTIIGMIIIGAIAGFLARALVPGKDSMSIPATILLGIVGSFV
                     GGFLASLLFNSGSGDFLRPSGLIGSVIGAIIALLIYNATAGRKGRTHSRSRV"
     gene            complement(468..695)
                     /locus_tag="VGB85_22195"
     CDS             complement(468..695)
                     /locus_tag="VGB85_22195"
                     /inference="COORDINATES: protein motif:HMM:NF022749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 domain-containing protein"
                     /protein_id="HEY0136816.1"
                     /translation="MEVKIGVQFAPRELSLESSQTPEEVEKAVADALKADLGVLTLVD
                     EKGRRVLVPADKLAYVEIAENEQRRVGFAAL"
     gene            complement(815..1450)
                     /locus_tag="VGB85_22200"
     CDS             complement(815..1450)
                     /locus_tag="VGB85_22200"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0136817.1"
                     /translation="MTTAEDGRTPRGTRLARPARRKQLLGAAHELFVAHGYHAAAMDD
                     IAERAGVSKPVLYQHFPSKLELYCALLDQHAEDLLAKLRGALDSTTDNKARVAASVAA
                     YFDFVDGKGSDVEGAFRLVFESDLRNDPAVRERVERMTRGCADAIAETIAQDTGYRPE
                     EAQLLSIGLTGLMEVSARWWLTSPDRIPKDRAVELLLGLAWRGISGSPRAV"
BASE COUNT          213 a          563 c          593 g          234 t
ORIGIN      
        1 ccggaggccg gccacccgag ggtgaccggc ctccgcagtg cggggagtgc tacacgcggc
       61 tgcgcgagtg cgtgcggccc ttgcggccag cggtcgcgtt gtagatcagc agcgcgatga
      121 tggcgccgat gaccgagccg atgaggccgc tcggacggag gaagtcgcca ctgccgctgt
      181 tgaacagcag cgaggccagg aagcctccga cgaacgagcc gacgatgccg agcaggatcg
      241 tcgccgggat gctcatgctg tccttgccgg gcaccagggc gcgagccagg aagcccgcga
      301 tggcgccaat gatgatcatg ccgatgatgg tgccgatcat gggtcctcct cgatgtgcgg
      361 ggcctacgcc cgttcgagga cctgtgtgcc cacttgcacg atcagcgaac ctgtggtcgt
      421 gcaggtcaca ggcctgtcac ccgttcggag cagtgccgac acacctgcta cagcgcggcg
      481 aagcccaccc ggcgctgctc gttctcggcg atctcgacgt aggcgagctt gtcggccggg
      541 accagcacgc ggcggccctt ctcgtcgacg agggtgagca ctccgaggtc ggccttgagc
      601 gcgtcggcga ccgccttctc gacctcctcg ggcgtctggc tgctctcgag gctcagctcg
      661 cggggcgcga actgcacgcc gatcttgacc tccacggagg gctcctcttc catcagggac
      721 gggtgcgtcg gatccacgct agtgcgtggg acccgagccg gcccgggcgt acgccgtggg
      781 cgcaacgccg tcgcagcgcc gccgcgaccg ggggtcagac cgcccgcggg gagccggaga
      841 tgccccgcca ggccagcccc agcaggagct cgacggcccg gtccttgggg atgcggtcgg
      901 gggaggtcag ccaccagcgg gcgctgacct ccatgagccc ggtcaggccg atcgacagca
      961 gctgcgcctc ctccggccgg tagccggtgt cctgggcgat cgtctcggcg atcgcgtcgg
     1021 cgcagccgcg ggtcatgcgc tcgacgcgct cgcggacggc cgggtcgttg cgcaggtcgc
     1081 tctcgaagac gagccggaac gccccctcga cgtcgctgcc cttgccgtcg acgaagtcga
     1141 agtaggcggc caccgaggcg gcgacgcgcg ccttgttgtc cgtggtgctg tccagcgcgc
     1201 cccgcagctt ggcgagcagg tcctcggcgt gctggtcgag cagcgcgcag tacagctcga
     1261 gcttgctcgg gaagtgctgg tagagcaccg gcttgctgac gccggcccgc tcggcgatgt
     1321 cgtccatcgc ggctgcgtgg tagccgtggg cgacgaacag ctcgtgcgcc gccccgagca
     1381 gctgcttgcg gcgggcggga cgggccaggc gcgtcccccg aggggtgcgg ccgtcctccg
     1441 ccgtggtcat ggctcgaccc taacggcgcc gccgagcctc agcggcgctc gtcctcgtcg
     1501 agctcgacga ccgctgcctg ctccgcggcg tcggcggggt cggcctccag cgggaggtcg
     1561 gcgacgaccg gcggctcggg cggaccggcg cccgcagcct gct
//
LOCUS       DASWNJ010001332         2665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_516730, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001332 DASWNJ010000000
VERSION     DASWNJ010001332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516730"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..2000)
                     /locus_tag="VGB85_22205"
     CDS             complement(120..2000)
                     /locus_tag="VGB85_22205"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEY0136818.1"
                     /translation="MRGPAAPKIAALERLIAAGVPVPAFVALADTAEPDTTAWLAIDA
                     ILLRGGPVIVRAALAGEDTADASGAGLGTSIAGCRDRDEVRAALRQIAATLHDPWLAD
                     YFSGPPRAQVLVQREIAGPWLAVVAQALLHLDEHDRPPPPASPAPFVPDAQPAAPARV
                     CHVELHRRDHSEPLAAGLSPALAGPLELFPADLREPIAHLCERVTAALPAATHGLDLE
                     LVADHEGRIWVVQARPLTRPLHPGWPAFAAAARLDLPASDLRDRLPLPGLWVLDAEHN
                     PTPLSPAHAGLIRRLTAEGGARMQVLAGWLYIPGKLAAPTPSSTTASLRAALTALQER
                     HIPDARAELARIDRLLDFTPLTTLLDLAHQHLRRVLATYATLPARPARLATTTTSLSL
                     HGREDHLDVLPTTWDIASPTLQDLAFETSPPGPLTPPDDPDVLATLLGELDDHLFALG
                     LAPLRRVYLCAAALLGLSTADIFLLTADELAAGALPDLEQRRASHASHASLFPPSQLH
                     DGLPVPASLTSHLRGVAIGPVAEGPLHLRRDLAELLADPPPPGAVLAIPALTAQAAVV
                     LRALKISAVCCEHGGALSHAALMARELGISALIGCRGCTEVPPGTHVRLDTRTGRLRV
                     RA"
     gene            2045..2545
                     /locus_tag="VGB85_22210"
     CDS             2045..2545
                     /locus_tag="VGB85_22210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136819.1"
                     /translation="MRGADQLPGPVLAEWLIHTLRPLRTAVLRGPEVALAGTGAMLHE
                     RVAAVAAPYGWTTVDGNTDMGIDFAALIVIRALGDAIVAPEVARAAARVQPGGQIIEL
                     AHPPAVRPWQVLRWLRRTRVMRSAAELRARAWLEFGLYGVEQWAPVDLPRLLVTCGRR
                     RAIPGR"
BASE COUNT          369 a          835 c         1146 g          315 t
ORIGIN      
        1 ccgccgatgc gccggcgaag cgcgcgaacc agggcgagcg agccgccgcc gcgacgccgc
       61 gcccccgacc ggcccgcgcg cgcgacgaga acctgtccga agacagcatc taaaaatttt
      121 caggcgcgca cgcgcagccg cccagtgcgc gtgtcgagcc ggacgtgggt gcccggtggg
      181 acctccgtgc agccgcggca gccgatcagc gccgagatac cgagctcgcg ggccatcagc
      241 gcggcgtgac tgagggcgcc gccgtgctcg caacacacgg ctgagatctt cagcgcgcgc
      301 aggacgaccg cggcctgggc ggtcaacgcg gggatcgcca gcacggcgcc cggtggtgga
      361 ggatctgcga gcagctcggc gaggtcgcgg cgcagatgca aggggccctc ggcgaccgga
      421 ccgatcgcca cgccgcgcag gtgactggtg agcgaagccg ggaccggcag gccgtcgtgg
      481 agctgtgacg gagggaagag agaggcgtgc gaggcgtgcg aggcgcggcg ctgctcgagg
      541 tcggggagcg cgcccgcggc gagctcgtcg gcggtcagga ggaagatgtc ggcggtcgag
      601 aggccgagca gcgccgcggc gcacaagtac acgcgtcgca gcggggccag accgagcgcg
      661 aacaggtggt cgtcgagctc gccgagcagc gtggcgagga cgtccgggtc gtcggggggt
      721 gtgaggggtc ctggcgggga tgtctcgaag gccaggtcct gcagcgtcgg gctggcgatg
      781 tcccaggtcg tcggcaatac gtcgaggtgg tcctcgcggc cgtgcaggga cagggaggtc
      841 gttgtcgtgg cgaggcgcgc gggtcgggcg gggagcgtcg cgtaggtcgc gagcacgcgg
      901 cgcaggtgtt ggtgggcgag gtcgaggagc gtggtgagcg gggtgaaatc gaggaggcgg
      961 tcgatgcggg cgagctcggc gcgcgcgtcg gggatgtgtc gctcctgcaa ggcggtgagc
     1021 gcggctcgca agctggcggt ggtggacgaa ggggtcgggg ccgcgagctt gccggggatg
     1081 tacagccagc cggcgagcac ctgcatgcgc gcgccacctt cggcggtgag gcggcggatc
     1141 aggccggcgt gcgccggtga gagcggggtg gggttgtgct cggcgtcgag gacccacagg
     1201 ccagggagcg gcaggcggtc gcggagatcg gaggcaggca ggtcgaggcg cgcggcggcg
     1261 gcgaaggcgg gccagcccgg gtgcagcggg cgcgtcagcg ggcgagcctg gacgacccag
     1321 atccggccct cgtgatcggc gacgagctcg aggtcgaggc cgtgcgtcgc tgccggcagg
     1381 gccgcggtga ctcgctcgca gaggtgtgcg atcggctcgc gcaggtcggc agggaacagc
     1441 tcgagcggtc cggcgagcgc ggggctgaga ccggctgcga gcggctcact gtggtcgcga
     1501 cggtgcagct cgacgtggca gacgcgggcc ggcgcggcgg gttgcgcgtc ggggacgaac
     1561 ggggcaggcg aggcgggtgg aggaggtcga tcgtgctcgt cgaggtgcag cagggcctgc
     1621 gcgacgacgg cgagccaggg gccggcgatc tcgcgctgca cgagcacctg cgcgcgcggc
     1681 gggccggaga agtagtcggc gagccagggg tcgtgcagcg tggctgcgat ctgccgtaac
     1741 gcggcgcgga cctcgtcgcg gtcgcggcag ccggcgatcg aagtgccgag gccggcgccc
     1801 gaggcatcgg ccgtgtcctc gccggccaac gcggcgcgca cgatcaccgg gccacccctt
     1861 aataaaatgg cgtcgatcgc gagccaggcg gtggtgtcgg gctcggcggt gtcggcgagc
     1921 gccacgaacg cgggcaccgg tacgcccgcg gcgatcagac gctcgagcgc ggcgatcttc
     1981 ggggcggctg ggcctcgcac gggcgcactg tagccggtcg cggcacccgt ggtagaggag
     2041 gcgggtgcgc ggcgccgatc agctcccggg tccggtgctc gccgagtggc tgatccacac
     2101 gctgcggccg ctgcggacgg cggtgctgcg cgggccggag gtcgccctcg cgggcacggg
     2161 cgccatgttg cacgagcggg tcgcggcggt ggcggctccg tacggctgga ccacggtcga
     2221 cggcaacacc gacatgggga tcgacttcgc ggcgttgatc gtgatccggg ccctcggtga
     2281 cgcgatcgtc gcgccggagg tcgcgcgggc ggccgcacgc gtgcaacccg gcgggcagat
     2341 catcgagctg gcccacccgc ccgccgtgcg accatggcag gtgctgcgct ggctgcgacg
     2401 cacgcgagtg atgcgctcgg cggccgagct gcgcgcgcgg gcgtggctcg agttcgggct
     2461 ctacggcgtg gaacagtggg ccccggtcga cctgccgcgc ctgctcgtga cctgcgggcg
     2521 ccggcgtgcg atacccggtc gatgaacgcg aacggagcga acgggccgga gacgctgtgg
     2581 gtcgaggtca gcgacgagcg cttcacggag ccggtgcagc gcagcttcaa gcgcgccggg
     2641 gagccgcagc acgtgaccgc ggtcg
//
LOCUS       DASWNJ010001333         7217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_200946, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001333 DASWNJ010000000
VERSION     DASWNJ010001333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200946"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VGB85_22215"
     CDS             <1..199
                     /locus_tag="VGB85_22215"
                     /inference="COORDINATES: protein motif:HMM:NF026037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136820.1"
                     /translation="DGRFTIAGVPPGTYTRVAWHSRGEAVRGEVTVTAGKAAGIEPAL
                     VETPQSSLHTRKDGSPYGRYN"
     gene            304..1338
                     /locus_tag="VGB85_22220"
     CDS             304..1338
                     /locus_tag="VGB85_22220"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEY0136821.1"
                     /translation="MAHPARRCGECHESHLTEWTGSSHAEADRSPVYQAMRAAAPDAA
                     ACDRCHAPLAAVLGRADPLAAEGVTCEVCHSIAAVELADEPGAAAGWTLQLAGNRKYG
                     PLCDVEEPYFHRAGCSPLHAASRLCAACHHLTQPGAAGVALPVFSEFAEWQHGEAMSA
                     GLQCQGCHMPKSGGTVASGGPRRVGVSQHGDVVAAGDALKVEAAVLARAGGLEVAGRV
                     RVSGVAHGLPAGVPGRELALVCELVDGTGRILASDEVTYGRVLVDDGGREVPFFAARR
                     VGADTRLHPGDARAFTLRLPAPPEGGAVVLRLLDRPISPSLARALAIAPPAARELSQR
                     RFTGPWQAAP"
     gene            1335..3125
                     /locus_tag="VGB85_22225"
     CDS             1335..3125
                     /locus_tag="VGB85_22225"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEY0136822.1"
                     /translation="MKLRTTLLLGMLALVAALVAATVLVLSVVLEREARRQIVGDLER
                     AEAVFADLHALRQSLYRSESLTVAQEPRLKAVLGTSDIDRATIVDVATEMQKAGGADL
                     FLLTDGAGRLIVDTAEPNTQNVELGELPLVRAALADGDAGGVWTQAQRVFQVHARRLA
                     FGDEVVGALVLGHRLDERVIRTVQRQTGSGVVLVLDGVAVDHALADPALAAAVAPLAS
                     ELGDGVHERTLLDDRYLVRRARLPGYAGDRALGVVFIESLDDALATSRALTRWISVLA
                     ALALVLAAGLAWAIARRLSRPVVELVEFTRNIAAGALEARAPLRGPVEVQALAAAMNH
                     MAGDLAAARLDLAAKERLEQELEIARRIQTSILPRHIIAPGMQLDARMRPASEVGGDY
                     YDILPVVDGCWLGIGDVAGHGLTAGLVMLMVQSLIAMLVQERPGARPSELLPALNMML
                     YKNIRERLSQDEHVTLTLLRVLADGQVYFAGAHEDIVVCRAASGRCERIETPGTWLGV
                     VAEIGPPGPDGQLRLAPGDLMLLYTDGVTEAMSTRREQFGMDRLCAALEQVRERPVGE
                     ICDHLMGVVAGWTAVQRDDVTLLVLRHTGA"
     gene            complement(3142..4443)
                     /locus_tag="VGB85_22230"
     CDS             complement(3142..4443)
                     /locus_tag="VGB85_22230"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEY0136823.1"
                     /translation="MLIVPLLAACTAEEELVDGLYAPAEWERIQTLAPLPDPPKDPTN
                     KYADDPAAAALGHKLFHEKGYSGAIVTEDLANGGLGKAGETGKISCASCHSGPWLIDE
                     RSKPGDNSLGSTWLPRNANAVVNAVYYLPWIENDGISDSLWSDALVDPEGIAMNGSRL
                     AVAHVLWDNYRDEYNAIFDPDLDPALDPAAPDAARFPPEGKPGVEAWEAMAPADQEHV
                     TRIFANFGKALDAYMRLLISRDAPFDRYVAGDMTAIDASAKRGLKLFIGKAACVECHD
                     GPHFDDSDDKFRVNGLRPEGSQVVPEETGRQFVIDYILANPFNGAGELSDDVAEGKRR
                     LAEISGLDAAAIAELNGKWRVKGLRQVAVTAPYMHTGQFKTLREVVEFYNDGGHPDGF
                     VGIKDPLMVPLNLSESEIDDLVAFLGTLTGEQVPAALIAPP"
     gene            complement(4578..6035)
                     /locus_tag="VGB85_22235"
     CDS             complement(4578..6035)
                     /locus_tag="VGB85_22235"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY0136824.1"
                     /translation="MTGKTIRIQRLRSPQALLAFLGLTVVGGGAAALGHIGSAEAAGE
                     TLWREGTGTPVITDNYEARRSLAPLIKEIGATVVNVRVAGKMRPAIDLDGIDPRMLPF
                     LDMGPQQKQGMGSGVILSSDGLVVTNHHVVDDAEKLEVKLADGRMFNAKVLGSDPLTD
                     IALIQLEGAKGLKAATLGSSATMSVGDWVLAIGSPMGLEQTATTGIVSAKGRGSLGLY
                     ANSYIDFLQTDAAIAPGSSGGPLFNMNGEVIGINTAVGAMGRGPGFAVPIDQAKQVLP
                     QLKHSGKVVRGWLGISGRDIEPAVGRTPETGAVIGAVVPGTPAAKAGLQNGDRIVGVD
                     GHDIASFGDLRGRIAEMKPGSEAALRVVRKDRPLTLKATVGKLPGDDELRKMSQRDER
                     GGKGGKGELFPDGQPRLGVEVEPGADGLQVRSVRAGSLADELGLRPGDQLQTINGEAI
                     RSTADVAAALGKDSGRVEVKVKRGSASHSAVIERR"
     gene            6167..>7217
                     /locus_tag="VGB85_22240"
     CDS             6167..>7217
                     /locus_tag="VGB85_22240"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0136825.1"
                     /translation="MTADSTPPTPPVAAPIVPAEDGSAAVPGPATAPARSATTIAWTS
                     STYFAEGLPWSVLHQIAAEFFTAAGLPPSQVGYTSALHLTSTLKFVWSPIVDLFGTLR
                     RWMISTQAVLGGLIGLLAVLAHDLALQTGDAKDTTFIWLTLIAIGVASATHDIACDGY
                     YMDALDKPSQARYTGVRIAAYRAAMLVGNAGLVYLGGRHGWLLGFGVAAALMVGLSVA
                     HRLMLPRGASERERVRAAAPRRTGAEKGAHIKEAYLSFLRQDRAVFVVLFLLTYKLGD
                     ALMFNMSKVLLRDLGVTTAERGVINGFGTIASIVGAMLGGAWIARTSLARAIVPITIL
                     MAATEPLYLLLAAPRR"
BASE COUNT          919 a         2558 c         2653 g         1087 t
ORIGIN      
        1 cgacggccgc ttcacgatcg ccggagtacc gcccggcacg tacaccaggg tcgcgtggca
       61 cagccgcggc gaggcggtcc gcggcgaggt caccgtcacc gccggcaaag cggcgggcat
      121 cgagccggcg ctcgtcgaga cgccgcagtc gagcctgcac acgcgcaagg acggttcgcc
      181 atacgggcgc tacaactgat ctgcctcgcc gcggcgctgg gctgtggggg atccggcgca
      241 cccgggcccg atgcgctgcc ggatcgagcc gagccggtcg atgaggtcgc ggtcgctgca
      301 gtcatcgccc atccggcacg ccggtgcggc gagtgccacg agtcccacct gaccgaatgg
      361 acaggctcat cgcacgcgga ggccgaccgc tcgccggtgt atcaggcgat gcgcgcggcg
      421 gcccccgacg cggcggcctg cgatcgctgc cacgcgccgc tcgccgcggt gctcggccgg
      481 gccgacccgc tcgccgccga gggcgtcacc tgcgaggtct gccactcgat cgccgccgtc
      541 gagctcgcgg acgagccggg cgccgccgcc ggctggaccc tgcaactggc cgggaaccgc
      601 aagtacggcc cgctgtgtga cgtcgaggag ccgtacttcc atcgcgccgg ctgctcgccg
      661 ctgcacgccg cgtcaaggct gtgcgccgcc tgccatcacc tcacgcagcc cggtgcggcc
      721 ggcgtcgcgc tgccggtgtt cagcgagttc gcggagtggc agcatggcga ggccatgtcc
      781 gccgggttgc agtgccaggg gtgccacatg ccgaagtccg gcggcacggt cgccagcggc
      841 gggccgcggc gcgtcggggt gtcgcagcac ggcgacgtcg tcgcggccgg tgacgcgttg
      901 aaggtcgagg ccgcagtgct ggcccgcgcg ggcgggctcg aggtcgccgg ccgcgtgcgc
      961 gtcagcggcg tcgctcacgg gctgccggcg ggcgtgcccg ggcgcgagct ggcgctggtc
     1021 tgcgagctgg tcgatgggac aggtcggata ttggcgagcg acgaggtcac ctacggccgc
     1081 gtgctcgtcg acgatggtgg ccgcgaggtg ccgttcttcg cggcgcgacg cgtcggcgcc
     1141 gatacccggc tgcacccggg cgacgcgcgg gcgttcacgc tgcggctgcc ggccccgccg
     1201 gagggcggcg ccgtcgtgct gcgtttgctc gaccgaccga tctcgccgag cctcgcgcgg
     1261 gccctggcga tcgcgccacc ggccgcgcgg gagctgtcgc agcggcgctt caccggaccc
     1321 tggcaggcgg ccccgtgaag ctgcgcacca ccctgctgct cggcatgctc gcgctggtcg
     1381 ccgccctggt ggccgcgacc gtgctggtgc tctctgtcgt gctcgaacgc gaggctcgca
     1441 ggcagatcgt cggcgacctc gagcgcgccg aggcggtgtt cgccgacttg cacgcgctgc
     1501 gtcagtcgct gtaccgctcc gagtcgctga cggtcgccca ggagccgcgc ctcaaggcgg
     1561 tgctcggcac cagcgacatc gaccgcgcga cgatcgtcga cgtcgccacg gagatgcaga
     1621 aggccggcgg cgccgacctg ttcttgctca ccgacggcgc gggccggctg atcgtcgaca
     1681 ccgccgagcc gaacacgcaa aatgtcgagc tcggcgagct gccgctggtc cgcgccgcgc
     1741 tggccgacgg cgacgccggc ggcgtgtgga cgcaggcgca gcgggtgttc caggtccacg
     1801 cgcggcgcct cgcgttcggc gacgaggtcg tcggcgcgct cgtgctcggg catcgcctcg
     1861 acgagcgtgt gatccgcacg gtgcagcgtc agaccggcag cggcgtggtg ctggtgctcg
     1921 acggtgtcgc cgtcgatcac gcgctcgccg accccgcgct ggcggccgcg gtcgcgccgc
     1981 tggcgagcga gctcggggac ggggtgcacg agcggacctt gctcgacgac cgctacctcg
     2041 tgcgtcgcgc tcgcctcccg ggctacgccg gcgatcgtgc gctcggggtc gtgttcatcg
     2101 agtcgctgga cgacgcgctg gcgaccagca gggcgctgac gcggtggatc tccgtgctcg
     2161 ccgcgctggc gctggtgctc gccgccggcc tcgcgtgggc gatcgcccgc cgtctttcgc
     2221 gcccggtcgt cgagctcgtc gagttcacgc gtaacatcgc cgccggggcg ctcgaggccc
     2281 gcgcgccgct gcgaggtccc gtcgaggtcc aggcgctcgc cgccgcgatg aaccacatgg
     2341 cgggcgacct cgcggccgcg cggctcgacc tcgcggcgaa ggagcgcctc gagcaggagc
     2401 tggagatcgc caggcggatc cagacctcga tcttgccgcg tcacatcatc gcacccggca
     2461 tgcagctcga cgcccgcatg cggccggcca gcgaggtcgg cggcgattac tacgacatct
     2521 tgccggtcgt ggacggctgc tggctcggca tcggcgacgt cgccggccac ggcctgaccg
     2581 ccggcctggt gatgctgatg gtccagagcc tgatcgccat gctcgtgcag gagcggcccg
     2641 gcgcgcggcc ctcggagctg ctgcccgcgc tcaacatgat gctctacaag aacatccgcg
     2701 agcgcctgtc gcaggacgag cacgtcacgc tgacgctgct gcgcgtgctc gccgacggcc
     2761 aggtgtactt cgccggcgcg cacgaggaca tcgtcgtgtg tcgggccgcg agcggccggt
     2821 gcgagcgcat cgagacgccc ggcacctggc tcggcgtggt cgcggagatc gggccgcccg
     2881 ggccggacgg gcagctgcgg ctcgcgcccg gcgacctcat gctgctctac accgatggtg
     2941 tcaccgaggc gatgagcacg cggcgcgagc agttcggcat ggatcgcctg tgcgccgcgc
     3001 tcgagcaggt ccgcgagcgg cccgtcggcg agatctgcga tcacctgatg ggcgtcgtcg
     3061 ccggctggac cgcggtccag cgcgacgatg tcacgctgct ggtgctgcgc cacaccggcg
     3121 cgtgagcacc tggccaaaag gtcagggcgg agcgatcaac gccgccggca cttgctcgcc
     3181 ggtcagggtg ccgaggaacg cgacgaggtc gtcgatctcc gactcgctca ggttgagcgg
     3241 gaccatcagc gggtccttga tgccgacgaa gccgtccggg tggccgccgt cgttgtagaa
     3301 ctcgaccacc tcgcgcagcg tcttgaactg cccggtgtgc atgtacgggg cggttacggc
     3361 gacctgacgc agccccttga cccgccactt gccgttgagc tccgcgatcg cggccgcgtc
     3421 gagcccggag atctccgcga ggcggcgctt gccctccgcg acgtcgtcgc tgagctcgcc
     3481 cgcgccgttg aacgggttgg cgaggatgta gtcgatgacg aactggcggc cggtctcctc
     3541 ggggaccacc tgcgagcctt cggggcgcag cccgttgacg cggaacttgt cgtcgctgtc
     3601 gtcgaagtgc gggccgtcgt ggcactcgac gcaggcggcc ttgccgatga acagcttgag
     3661 cccgcgcttg gccgaggcgt cgatcgccgt catgtcgccg gcgacgtaac gatcgaaggg
     3721 cgcgtcgcgg ctgatcagca ggcgcatgta ggcgtcgagc gccttgccga agttggcgaa
     3781 gatccgcgtg acgtgctcct ggtcggccgg ggccatcgcc tcccacgcct cgacgccggg
     3841 cttgccctcg ggcgggaagc gggcggcgtc cggcgcggcc gggtcgagcg cgggatcgag
     3901 gtccgggtcg aagatcgcgt tgtactcgtc gcggtagttg tcccacaaga cgtgcgcgac
     3961 cgcgaggcgg ctgccgttca tcgcgatgcc ctcggggtcg accagcgcgt cggaccacag
     4021 cgagtcgctg atgccgtcgt tctcgatcca cggcaagtag tacacggcgt tgacgaccgc
     4081 gttggcgttg cgcggcagcc aggtcgagcc gagcgagttg tcgccgggct tcgagcgctc
     4141 gtcgatcagc cacggcccgg agtggcagct cgcgcacgag atcttgccgg tctcgccggc
     4201 cttgccgagc ccgccattgg cgaggtcctc ggtgacgatc gcaccggagt agcccttctc
     4261 gtggaacagc ttgtggccga gcgcggcggc ggccggatcg tcggcgtact tgttggtcgg
     4321 atccttcggc ggatcgggca gcggggccag ggtctggatg cgctcccact ccgctggcgc
     4381 gtacagcccg tcgacgagct cctcctccgc cgtgcaggcg gcgagcaggg gcacgatgag
     4441 catcagacga gcgacgaacg aaggcagggc cggacagctg tccatggcgt cactatcgcg
     4501 gcaatgcatg gcgataagca aggcaatacg ggccccccac gtgtgcaggg ggccagcgca
     4561 tcggctcgca tcggcgatta tcggcgttcg atgaccgccg agtggctcgc gctgccgcgc
     4621 ttgaccttga cctcgacgcg gccgctatct ttgccgagcg ccgcggcgac atcggcggtg
     4681 ctgcggatcg cctcgccgtt gatcgtctgc agctggtcgc cgggccgcag gccgagctcg
     4741 tcggccagcg agccggcacg gacgctgcgg acctggagac cgtctgcgcc gggctcgacc
     4801 tcgacgccga ggcgcggctg gccgtccggg aacagctcgc ccttgccgcc cttgccgccc
     4861 cgctcgtcgc gctggctcat cttgcgcagc tcgtcgtcgc ccggcagctt gccgacggtc
     4921 gccttgagcg tcagcgggcg gtccttgcgg acgacccgca gggcggcctc ggagccgggc
     4981 ttcatctcgg cgatgcggcc gcgcaggtcg ccgaagctgg cgatgtcgtg gccatcgacg
     5041 ccgacgatgc ggtcgccgtt ctgcaggccg gccttggcgg cgggcgtgcc gggcacgacg
     5101 gcgccgatca ccgcgccggt ctcgggggtc cggccgaccg cgggctcgat gtcgcgaccg
     5161 ctgatgccga gccagccgcg cacgaccttg ccgctgtgct tgagctgcgg gaggacctgc
     5221 ttggcctgat cgatcgggac cgcgaagccg ggaccgcgac ccatcgcgcc gacggccgtg
     5281 ttgatgccga tcacctcgcc gttcatgttg aacagcggac cgccgctcga gcccggggcg
     5341 atcgcggcgt cggtctgcag gaagtcgatg tagctgttgg cgtagaggcc gaggctgccg
     5401 cggcccttgg ccgagacgat gccggtcgtc gccgtctgct cgaggcccat cggcgagccg
     5461 atcgccagca cccagtcgcc gaccgacatc gtcgcgctcg agccgagagt ggccgccttg
     5521 agccccttgg cgccctcgag ctggatcagc gcgatatcag tcaaggggtc ggagccgagc
     5581 accttggcgt tgaacatgcg gccgtcagcg agcttgacct cgagcttctc ggcgtcgtcg
     5641 acgacgtggt ggttggtgac gacgaggccg tcgctgctga ggatcacgcc ggagcccatg
     5701 ccttgcttct gctgcgggcc catgtcgagg aagggcagca tgcgcgggtc gatgccgtcg
     5761 aggtcgatcg ccgggcgcat cttgccggcg acgcgcacgt tgacgaccgt cgcgccgatc
     5821 tctttgatta gaggcgcgag gctgcggcgc gcttcgtagt tgtcggtgat caccggggtg
     5881 ccggtgccct cccgccacag cgtctcgccc gccgcctccg cgctgccgat gtggccgagc
     5941 gcggcggcgc caccaccgac caccgtcagc ccgaggaagg cgagcagggc ctgcggcgag
     6001 cgtaacctct ggatgcggat cgtcttgcca gtcatgcccg cgacaaccgc gggtccgcgc
     6061 cgcctattcc cgccacgtgt cccctgcggg cccctgggcg gcccggcgag gcgcccgcaa
     6121 cggcaagaac ttggcgtgat cgatctgggg tgtactctcg cccgccgtga ccgctgactc
     6181 gacgccgccg acgccgcccg tggccgcacc gatcgtgccc gcagaggacg gcagcgccgc
     6241 agttcccggg ccggcgaccg cgccggcgcg cagcgcgacg acgatcgcgt ggaccagctc
     6301 gacctacttc gccgagggcc tgccgtggtc ggtgctgcat cagatcgcgg ccgagttctt
     6361 cacggcggcc ggcctgccgc cctcgcaggt cggctacacc tcggcgctgc acctcacgag
     6421 cacgctcaag ttcgtgtgga gcccgatcgt cgacctgttc gggaccctgc ggcggtggat
     6481 gatctcgacc caggcggtgc tcggcggcct catcggcctg ctcgccgtgc tcgcacacga
     6541 cctggccctc cagacaggtg acgccaagga cacgaccttc atctggctga cgctcatagc
     6601 gatcggcgtc gcctcggcga cgcacgacat cgcctgcgac ggctactaca tggacgcgct
     6661 cgacaagccg agccaggccc gctacaccgg cgtgcgcatc gcggcctatc gcgccgcgat
     6721 gctcgtcggc aacgccggcc tcgtgtacct cggcgggcgg catggctggt tgctcggctt
     6781 cggcgtcgcg gcggccctga tggtcgggct gtcggtcgcg caccgcctga tgttgccgcg
     6841 cggcgcgagc gagcgggagc gggtgcgggc ggccgcgccg cggcggaccg gcgcggagaa
     6901 gggcgcgcac atcaaggagg cgtacctcag cttcctgcgc caggaccgtg cggtgttcgt
     6961 ggtgttgttc ctgctgacct acaagctcgg cgacgccctg atgttcaaca tgtcgaaggt
     7021 gctgctgcgc gacctcggcg tgacgaccgc ggagcgcggc gtgatcaacg gcttcgggac
     7081 catcgcgagc atcgtcgggg cgatgctcgg cggggcgtgg atcgcccgca cctcgctcgc
     7141 gcgggcgatc gtgccgatca cgatcctgat ggcggcgacc gagccgctgt acctgctgct
     7201 cgccgcgccg cggcgga
//
LOCUS       DASWNJ010001334         4013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_307192, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001334 DASWNJ010000000
VERSION     DASWNJ010001334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307192"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            459..1526
                     /locus_tag="VGB85_22245"
     CDS             459..1526
                     /locus_tag="VGB85_22245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136826.1"
                     /translation="MITSVSDGSSTGSTSSSSSSGTTTTTGESGPGDGDTHAEPPRDL
                     GTMPDFGDGAPAGCAGKIDFLFVISRQELMGERQAQLALAVPQFIDTIKTKFANFDYH
                     IMVVDGDGPTSVGGWGSMSCNEVCPNLACKVGEDCCSIGNSKLDGMPCCDDPSYPCDK
                     LDLVTACDEAWGAGTVFPAGTYTADKPCPIDGGRRYLVMGQTDLDKTFECIARVGNSG
                     YGMLGQAFAAAMQEDINEPGGCNAGFLRKDALLMVTFIAANPDWGDATGSDGTPAKWA
                     KAVIDAKHGDKNSVVMFNIGACDCGPYEGECHPVDRLCVLSAMFPYHHSEYVGVADYG
                     PAFDKAASLVETACAGFVVPG"
     gene            complement(1536..3149)
                     /locus_tag="VGB85_22250"
     CDS             complement(1536..3149)
                     /locus_tag="VGB85_22250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136827.1"
                     /translation="MNIRIDPVLVLPLAVLLGCPLPAEQVTCEEEDACGTTQPAAVTG
                     DGPPTTSAGVQTMTGASADAGSTLPDAETGAESTTGQPAEPPEIVTVAVIPKFIDKNG
                     LIEVAIMAEHADGVRMAIDTGEPIELTPIGPGELGGQIPAFTGLDNGEHTVIFTPWRN
                     TLVGESVAANYVIALPPPGYEVSWQTTDIEGHVAAIDVLPDGRPVELGTYEEMGEPHC
                     YLQLREETGEPVEFFPLLPLAHCTAIDLKIDRDTGLMHVLLRRKSGDGLRWWAGEIPG
                     WGAGPKNIGIGEVGDTALALASRPGLVAVCGAKPVATPEGLDGLVVLLRPNQPAEERL
                     FDYVPAVHDHKFKETIRDCTFTGDTLVLVGEASGVHGGEDQERERLTLLEHNVVADTE
                     VWTVAGPGPGLQSRALAVDADDEGRYHLAGYTCLDACAPEGEVRVYEHGGKLVDQIPL
                     GPLGSDWSGPHDIAWSPAGYAVVALGEQQGQSFVFKVQAFAPGVPGPLWTFTPNDKQA
                     LQIAFAVAVGPFGEIYAGGVGPTFAVIGG"
     gene            complement(3170..>4013)
                     /locus_tag="VGB85_22255"
     CDS             complement(3170..>4013)
                     /locus_tag="VGB85_22255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136828.1"
                     /translation="DRVEYLYFAHRAYLALAKKAPASATHDLCKAKQLIDQALALPGT
                     ELRERLVASRQETLSRLTEQRAQCRQPSKARKNVALAAPRPAVKTPDPPAGAANPVKP
                     GVSVASDAPTADVTTSLVSAAPAAATPDLMPVTARRVPRPSVVDEPPPRHLVPTRRVD
                     EPRPGRDLVIAGGVTLGLGVGLSVAAGVMGHRLAEKRRDVVALDERIDDYATPTQDAK
                     DAALRREYEAMKPPTVALAITAGATLVVAVILTTIGARRMARSASRTVLAPAPGGLVF
                     HARF"
BASE COUNT          595 a         1406 c         1404 g          608 t
ORIGIN      
        1 cgagcacggc gccggtatcc tccggatcgt cgaccgcgaa catcgcggaa ccgcgccggg
       61 tccagccgat gctggcgccg ttaccgtgga cgaagcgctc ggtgcagcag ccggcccctg
      121 gcgcgcacgc tgcatcgcac gggtcggatc cgcgcggggt gaccacgtcg aacgcggatg
      181 gtccggctgg atcgcacgcg ggagcgaaaa cgacaaagcc cgccaggagt ggcaaagggg
      241 acgcccggtg catcatccga ctgcaccacc cgcgcaccac gctgtcaact tgatgcagag
      301 gtcttcatga aaccaaacgc gcattttcgc acgatcgggg gtggccagcg ggcccgaggc
      361 gcgttatcgt ccatgctgtg aggctcccgc tcccgctcct cgtccttggc gcctgtcagt
      421 cgccggccgc ggacacatct ccgttcacga cgacgcccgt gatcacgtcc gtgtccgacg
      481 gatcgtcgac cggctcgact tcgagctcga gcagctccgg gacgacgacg acgaccggtg
      541 agagcggccc gggcgacggc gacacgcacg ccgagccgcc tcgcgatctc ggcacgatgc
      601 cggacttcgg cgacggcgcg cccgccggct gcgcggggaa gatcgacttc ctgttcgtga
      661 tctcgcggca ggagctcatg ggggagcgcc aggcccagct cgccctggcg gtgccgcagt
      721 tcatcgacac gatcaagacg aagttcgcga acttcgacta tcacatcatg gtcgtggacg
      781 gcgacggccc gacgtccgtg ggaggatggg gcagcatgag ctgtaacgag gtctgcccga
      841 acctcgcctg caaggtcggc gaggactgct gctcgatcgg gaactccaag ttggatggca
      901 tgccgtgctg cgatgatccg agctacccct gcgacaagct cgatctcgtg accgcctgcg
      961 acgaggcgtg gggcgccggc acggtgttcc ccgccggcac ttacacggcg gataagccct
     1021 gtccgatcga cggcggtcgc cgttacctcg tgatgggcca gaccgatctg gacaagacct
     1081 ttgagtgcat cgccagggtc ggtaacagcg gctacggcat gctcgggcag gcattcgccg
     1141 ccgcgatgca ggaggatatc aacgaacccg gcggctgcaa cgcggggttc ctgcgcaagg
     1201 acgcgctgct gatggtgacg ttcatcgccg ccaacccgga ctggggcgat gcgaccggct
     1261 cggacgggac gccggccaag tgggccaagg cggtgatcga cgccaagcac ggcgacaaga
     1321 actcggtggt gatgttcaac atcggcgcat gtgactgcgg gccgtacgag ggcgagtgcc
     1381 accccgtgga ccggctctgt gtgctgagcg cgatgttccc gtaccaccac agcgaatacg
     1441 tgggggtcgc cgactatggg cccgcgttcg acaaagcggc gagcctcgtc gagaccgcgt
     1501 gcgccggttt cgtggtgccg ggctaaagcc cggccctacc cgccgatgac ggcgaacgtc
     1561 ggaccgacgc ctcccgcgta gatctcgccg aacggaccca ccgccaccgc gaaggcgatc
     1621 tgcagggcct gcttgtcgtt tggggtgaag gtccacagcg gccccgggac gcccggcgcg
     1681 aacgcctgca ccttgaacac gaaggactga ccctgctgct cgccgagggc gacgacggcg
     1741 tagcccgccg gactccacgc gatgtcgtgc gggccggacc agtccgaccc gagcgggccg
     1801 agcgggatct ggtcgacgag cttgccgccg tgctcgtaga ccctgacctc gccctccggt
     1861 gcgcaggcgt cgaggcaagt gtagccggcg aggtggtagc ggccctcgtc gtccgcgtcg
     1921 accgcgaggg cccggctctg gaggccgggt cctggtccgg ccacggtcca cacctccgtg
     1981 tcagccacga cgttgtgctc gagcaacgtg agacgctcgc gctcctggtc ttccccgccg
     2041 tgcacgccac tggcttcacc gacgagcacg agcgtgtcgc ccgtgaacgt gcagtcgcgg
     2101 atggtctcct tgaacttgtg gtcatgcacc gcgggcacgt agtcgaacag ccgctcctcc
     2161 gccggctggt tcggccgcag cagcacgacg agcccgtcga gtccctcggg cgtcgctacc
     2221 ggcttcgcgc cgcagaccgc caccaggccg ggccggctcg ccagcgcgag cgccgtatca
     2281 ccgacctcgc cgatgccgat gttcttcggt ccggcgcccc acccggggat ctcgccggcc
     2341 caccaccgca ggccgtcgcc gctcttgcgt cggagcagca cgtgcatcag gccggtgtcg
     2401 cggtcgatct tcaggtcgat cgcggtgcag tgtgcgagcg gcaacaaggg gaagaactcg
     2461 accggctccc cggtctcctc gcgcagctgg aggtagcagt gcggctcgcc catctcctcg
     2521 taggtgccga gctcgacggg gcgaccgtcc ggcaacacgt cgatcgcggc gacgtgcccc
     2581 tcgatgtcgg tcgtctgcca cgacacctcg tagcctggcg gcggcagggc gatcacgtag
     2641 ttcgccgcga ccgactcgcc gacgagggtg ttgcgccacg gtgtgaagat caccgtatgc
     2701 tcgccgttgt cgagcccggt gaacgccggg atctggccgc cgagctcgcc ggggccgatc
     2761 ggcgtcagct cgatcggctc gccggtgtcg atcgccatcc gcacgccgtc cgcgtgctcc
     2821 gccatgatcg cgacctcgat cagaccgttc ttgtcgatga acttgggtat caccgccacg
     2881 gtgacgatct ccggcggctc ggcgggctgg ccggtcgtac tctccgcacc cgtctccgca
     2941 tcggggagcg tggagccggc gtcggcgctg gcgcccgtca tcgtctggac ccccgcgctc
     3001 gtcgtcggtg ggccatcgcc cgtgacggcg gccggctgcg tggtgccgca cgcgtcttct
     3061 tcctcgcaag tcacctgctc ggccggcagc gggcacccca ggagaaccgc gaggggcagg
     3121 acaaggaccg gatcgatacg gatgttcacg gggtctccgg gacaggaggt cagaaacgcg
     3181 cgtggaacac aagccccccg ggagccggcg ccagcacggt gcgcgaggcg gagcgggcca
     3241 tccgtcgcgc accgatggtg gtcaggatca cagcgacgac gagggtcgcc ccggcggtga
     3301 tcgccagcgc gacggtcggg ggcttcatcg cctcgtactc gcgccgcaag gcggcgtcct
     3361 tcgcgtcctg ggtcggtgtc gcgtagtcgt cgatcctctc gtcgagcgcg acgacgtccc
     3421 gacgcttctc ggccaggcgg tgccccatga cccccgccgc cacgctgagg ccgaccccaa
     3481 ggccgagtgt caccccgccc gcgatcacga gatcgcgacc cgggcgtggc tcgtccacac
     3541 ggcgggtcgg gacgaggtgt cgcgggggcg gctcatccac cacgctgggc cggggcacac
     3601 gccgggccgt caccggcatc aggtccggcg tcgcggcagc tggcgccgcc gacacgagcg
     3661 aggtggtcac gtccgcggtc ggggcgtcgc tggcgaccga cacgcccggc tttaccgggt
     3721 tcgcggcgcc ggccggcggg tccggggtct tgaccgcggg tcgcggcgcc gcgagtgcga
     3781 cgttcttcct ggccttgctc ggctggcggc actgggcccg ctgctccgtg agccgcgaca
     3841 gcgtctcctg cctggacgcg acgaggcgct cgcgcagctc ggtcccgggg agcgcaagag
     3901 cctggtcgat caactgcttc gccttgcaca ggtcgtgtgt cgccgaggcg ggcgccttct
     3961 tcgcgagcgc gaggtaggcc cggtgtgcga agtacaggta ctccactcga tcc
//
LOCUS       DASWNJ010001335         1761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_59909, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001335 DASWNJ010000000
VERSION     DASWNJ010001335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59909"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..>1761
                     /locus_tag="VGB85_22260"
     CDS             110..>1761
                     /locus_tag="VGB85_22260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEY0136829.1"
                     /translation="MTPASSTSLGADGGGAKTHGWGVSLRFRGRPLVERSVRDPAPLR
                     IGEDPACTLVVPGLGDASTLANADRLHAVPGLTGVVHRGAGQGSEPMGRELTLAPGDH
                     ASFGFVDHPEITLELRRETYPRLPLSTLINLRELAQQLMLGAGLVAGLILLVRHEAPV
                     NTLEVKGDPDAPEESALVRAMFAVEVPPIELRYHQLWAPIPAPAPAVVGPVEPIAVEP
                     EAAPIPMLVEAEAVPVPSAPADAQPRKRKKRGEALEMQMLSALVASDDDSLGGVVGGV
                     LSDGVGNDLLAELRSDAPLAAGTGGYGEGIVPTDEAVVEVVEEKVVDLVGAIADVEVV
                     EPVEFMENIMGPGVPDAAPPDAVPVPKDIPTPPGMNRYPIDGTAVVTRGVIDGLAAAA
                     PESRCEDPALVRQSQLDVVFVLDVSTTMTFMLDRIEKQIAQVDLETRAQGLDTRYGLV
                     VFVDDIKLGNAGKPYADLAALQAELATWRAFTAGNRQINSPDLNLDWPENTLDAVHAA
                     ATEFAWRPADTTLRMIVHATDDDFGEAPAIQSGQPVKHGYAAT"
BASE COUNT          250 a          686 c          597 g          228 t
ORIGIN      
        1 cccggagacc tcgcggccgc ctcccgccga aaccggcgcc ggagaggccg ccgcgacgcc
       61 cgtgacgccg gcctcctccc cggcttccac cccggcttcg accccggctt tgaccccggc
      121 ttcgtcgacc tccctcgggg ccgacggcgg gggcgcaaag acccacggtt ggggcgtcag
      181 cctgcgtttt cgcgggcgcc ctctggtcga gcgcagcgtc cgggacccgg caccgctgcg
      241 catcggcgag gatcccgcgt gtaccctcgt cgtccccggc ctcggcgacg ccagcaccct
      301 ggcgaacgcg gaccgcctgc acgccgtgcc tggcctgacc ggcgtcgtgc accgcggggc
      361 ggggcagggc agcgagccca tgggtcgtga gctgacgctg gccccgggtg atcacgccag
      421 cttcggcttc gtcgaccacc cggagatcac cctcgagctg cgccgcgaga cctatccgcg
      481 cctgccgctc tcgacgctga tcaacctccg cgagctcgcg cagcagctga tgctcggcgc
      541 cggcctggtc gctggcctga tcctgctggt ccgtcacgag gcccccgtca acacgctcga
      601 ggtcaagggc gacccggacg cgcccgagga atcggccctc gtgcgggcga tgttcgccgt
      661 cgaggtcccg ccgatcgagc tgcgctacca ccagctctgg gccccgatcc ccgcgccggc
      721 gcccgcggtc gtcgggccgg tcgagcccat cgccgtcgag cccgaggccg cgccgatccc
      781 gatgctcgtc gaggccgagg ccgtgccggt cccatccgca cccgccgacg cccagccccg
      841 caagcgcaag aagcgtggcg aagccctcga gatgcagatg ctcagcgcgc tggtcgcgag
      901 cgacgatgac agcctcggcg gcgtggtcgg cggcgtgctc agcgacggcg tcggcaacga
      961 cctgctcgcc gagctccggt ccgacgcgcc cctggccgcc ggtacgggcg gttatggcga
     1021 gggcatcgtg cccaccgacg aggcggtcgt cgaggtcgtc gaggagaagg tggtcgacct
     1081 ggtcggggcc atcgcggacg tcgaggtcgt cgagcccgtc gagttcatgg agaacatcat
     1141 gggccccggt gtgcccgacg cggcgccgcc cgacgcagta cctgtcccga aggacatccc
     1201 gacgccgccg ggcatgaaca ggtacccgat cgacggcacg gccgtggtca cgcgcggcgt
     1261 gatcgacggc ctcgcggcgg ccgcccccga gtcgcgttgc gaggacccgg cgctggtccg
     1321 ccagagccag ctcgacgtcg tgttcgtcct cgatgtatcg acgacgatga ccttcatgct
     1381 cgaccgcatc gagaagcaga tcgcgcaggt cgacctcgag acgcgcgcgc agggcctcga
     1441 cacccgctac ggcctggtcg tgttcgtcga cgacatcaag ctcggcaacg ccggcaagcc
     1501 ctacgccgac cttgcggcgc tgcaggccga gctcgccacg tggcgggcct tcaccgccgg
     1561 caaccgccag atcaacagcc ccgacctcaa cctcgactgg cccgagaaca ccctcgacgc
     1621 cgtgcacgcg gccgcgaccg agttcgcctg gcgccccgcc gacacgaccc tgcggatgat
     1681 cgtgcacgcg accgacgacg acttcggcga ggcccctgcg atccagagcg gacagcccgt
     1741 caagcacggc tacgccgcga c
//
LOCUS       DASWNJ010001336         1649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_24798, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001336 DASWNJ010000000
VERSION     DASWNJ010001336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24798"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="VGB85_22265"
     CDS             complement(<1..1212)
                     /locus_tag="VGB85_22265"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEY0136830.1"
                     /translation="MSAQPPTDPEDRASSGIPGLDDILGGTGFPRNQMYLVEGNPGAG
                     KTTLGLHFLMEGVRRGEKVAYIVLAESEKALRRLARSHGWDLTDVIVIESADQSGNDD
                     QYSLFHPSEIELGEASDTILGEIDRLRPSRVVLDSLSEVRLLAQDPLRYRRQILTLRK
                     FFDDRNITALLLDFVSDHDDRQLESLCHGIVQLSQRTPEYGGHRRRLRVTKLRESAFR
                     DGYHDFTIDRGGIQVFPRLVASEHGRGLGFKQETCSSGVPELDLLCGGGLDRGTSTLI
                     LGPAGAGKSTLSAVYAKAAVERGEKVAFFVFDEVPNTLIVRGEGMSLNVRAQVASGHM
                     IIRQVDPAELAPGQFAHQVRHAVEVLGASVVVIDSVNGYHSAMPEENFLSAHLHELLA
                     YLNQRNIVSIMV"
BASE COUNT          250 a          520 c          616 g          263 t
ORIGIN      
        1 caccatgatg ctgacgatgt tgcgctggtt gaggtacgcg agcagctcgt gcaggtgcgc
       61 gctgaggaag ttctcctcgg gcatcgcgct gtggtagccg ttgacgctgt cgatgacgac
      121 gacgctggcg ccgaggacct cgacggcgtg gcggacctgg tgggcgaact gaccgggggc
      181 gagctcggcg gggtcgacct ggcggatgat catgtggccc gaggcgacct gcgcgcgcac
      241 gttgaggctc atgccctcgc cgcggacgat cagcgtgttg gggacctcgt cgaacacgaa
      301 gaaggcgacc ttctcgccgc gctcgaccgc ggccttggcg tagaccgccg agagcgtcga
      361 cttgccagcg ccggcgggcc cgaggatcag cgtgctggtg ccgcggtcga ggccgccgcc
      421 gcacagcaga tccagctcgg gcacgccgct cgagcaggtc tcctgcttga agccgaggcc
      481 gcggccgtgc tcgctggcga ccaggcgcgg gaacacctgg atgccgccgc ggtcgatcgt
      541 gaagtcgtgg tagccgtcgc gaaacgccga ctcgcgcagc ttggtgacgc gcaggcgccg
      601 gcggtggccg ccgtactcgg gggtgcgctg gctgagctgg acgatgccgt ggcacaggct
      661 ctcgagctgg cggtcgtcgt ggtcgctgac gaagtcgagc agcagggcgg tgatgttgcg
      721 gtcgtcgaag aacttgcgca gcgtcaggat ctgccggcga tagcgcaggg ggtcctgggc
      781 gagcaggcgg acctccgaca gcgagtcgag cacgacccgg ctcgggcgca ggcgatcgat
      841 ctcgccgagg atcgtgtcgc tggcctcgcc gagctcgatc tccgaggggt gaaacaggct
      901 gtactggtcg tcgttgccgc tctggtcggc agactcgatg acgatgacat ccgtgaggtc
      961 ccagccgtgc gaccgggcca gccggcgcag cgccttttca ctctcggcga gcacgatgta
     1021 cgcgaccttt tcgccgcgac gcacgccttc catcaggaag tgcaggccga gagtcgtctt
     1081 gcccgcgccc ggattaccct cgaccaggta catctggttg cgcggaaacc cggtgccgcc
     1141 gaggatatcg tcgaggccgg ggatgccgga ggaggcccgg tcttcagggt cggtgggggg
     1201 ttgagcagac atggaggcta cgccggaaga cgctcgggac ccaacgacgc atagcatggc
     1261 tcgccgcgaa cgatgtctgg ccgcggtggg ctcgcctcgc cgccgggaaa tgccaccagg
     1321 gagcgtgtag agcggtgatg gcgtgggtag cgcggcgcga ggtcgcgccg gacaggtgcc
     1381 cgtaattttc cgcggatgtg ccaagacttc ggggcaagac gggccgcgga tcggccggca
     1441 gccatctccg ggagagcccg ggatcaggag tgcgagaacg cgcgttaagg ggtatggacc
     1501 atgagcgtca tgacccccgt tctccatctc ctggtcgccc tgacgatcgc tgcgcccccg
     1561 gtcgacccct cgacgtgcgc cgccgatgac ctgcaatgtt cggcgcctgc ttttgctgcc
     1621 gcggcccggc gcgcgcggtc caacgtgga
//
LOCUS       DASWNJ010001337         1572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_200976, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001337 DASWNJ010000000
VERSION     DASWNJ010001337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200976"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VGB85_22270"
     CDS             complement(<1..438)
                     /locus_tag="VGB85_22270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0136831.1"
                     /translation="MRILVIEDNQDIAANLGDFLEDRGHTVDFAADGITGLHLAVVHD
                     FDAIVLDLNLPGLDGLEVCRKLRNEARKQTPVLMLTARDSLDNKLAGFDSGADDYLIK
                     PFALQEVEVRLAALSRRGRGVQTRVLNVADLEYNLDTLEVRRGG"
     gene            complement(751..>1572)
                     /gene="rimK"
                     /locus_tag="VGB85_22275"
     CDS             complement(751..>1572)
                     /gene="rimK"
                     /locus_tag="VGB85_22275"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF007764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HEY0136832.1"
                     /translation="ARVLDPLRCYMRIACDSFDMHYKGKPLTGYDAVIPRIGASVTRY
                     GTAVLRQFELMGTCTPNPSDAILKARDKLRAHQLLAAQGIHMPVTAFGDNPDDTSDLL
                     NLLGRPPHVIKLTESTQGAGVILAEKLSASRGITEALRGLYANFLVQEFIAEAKGADV
                     RCFVVGDRVVAAMRRQAPAGEFRSNLHRGGSAVGVKPSRAECDVALRATQVLGLGVAG
                     VDLIRSERGPLVLEVNASPGLEGIEAASGKDVAGEIIKFVAQGCKPRRTVRAPRK"
BASE COUNT          239 a          514 c          556 g          263 t
ORIGIN      
        1 gccgccgcgg cgcacctcga gggtgtcgag gttgtattcg aggtccgcga cgttgagcac
       61 gcgcgtctgc acgccacgac cgcggcgcga gagcgcggcc agccgcacct ccacttcctg
      121 cagcgcgaac ggcttgatga ggtagtcgtc ggcgccggag tcgaaaccgg ccagcttgtt
      181 atcgaggctg tcgcgcgcgg tgagcatcag caccggcgtc tgcttgcgcg cttcgttgcg
      241 cagcttcctg cacacctcca ggccgtcgag gcccggcagg ttgaggtcga ggacgatggc
      301 gtcgaagtcg tgcaccaccg cgaggtggag cccggtgatg ccatcggcag cgaaatcgac
      361 ggtatgtccg cggtcttcga ggaagtcgcc gaggttcgcg gcgatgtcct ggttgtcctc
      421 gatgacgaga atgcgcattg agctttcctg gggctggagt cgcgcagcgc cggctaccgg
      481 acgggcggcc atggaggcgg ggcgtgtgtc gtagcgggga gtatgcattc gtccgattct
      541 cgcagaggcg gttcatgcgc catgttcaca tcggaccccg tcgcccaaaa aaagtgccca
      601 ggcacgtggc ctgggcaaaa agtgttaccc cgattaccgc ggaccacccg ggtcggccga
      661 ccacggggcg aggagcgcgg tccgggcacg ctacgcccgg aaggcttaaa cgcgagttaa
      721 gaaccggccc cgttttttgt tagggatccg ttatttgcgg ggcgcacgca ccgtccgcct
      781 tggcttgcag ccctgggcga cgaacttgat gatttctcct gccacgtcct tgccggaggc
      841 cgcctcgatc ccctccagcc cgggcgacgc attgacctcg agcaccagcg ggccgcgctc
      901 ggaacggatg aggtcgacgc ccgcgactcc aagaccgagc acctgggttg cccgcagcgc
      961 gacgtcgcac tccgcccggc tgggcttcac cccgaccgcg ctgccgccac gatgcaggtt
     1021 ggagcggaac tcccccgccg gggcctggcg gcgcatcgcg gccaccacgc ggtcccccac
     1081 gacgaagcag cgcacgtccg cgcccttggc ctcggcgatg aactcctgca ccaggaagtt
     1141 ggcatagagg ccacgcaggg cttcggtgat gccgcgggag gcggacagct tctcggcgag
     1201 gatgactccg gccccctggg tgctttcggt cagcttgatc acgtgcggcg ggcggccgag
     1261 caggttcagc aggtcgctgg tgtcgtccgg gttgtcgccg aaggccgtca ccggcatgtg
     1321 gatgccctgc gctgcgagca gctggtgcgc gcggagcttg tcgcgcgcct tgaggatcgc
     1381 gtcggacgga ttgggcgtgc aggtgcccat cagttcgaac tggcgcagca ccgcggtgcc
     1441 gtagcgcgtg accgacgcgc cgatgcgcgg gatcaccgcg tcgtagccgg tcagcggctt
     1501 gcccttgtag tgcatgtcga agctgtcgca ggcgatccgc atgtagcagc gcagcgggtc
     1561 gagcacgcgt gc
//
LOCUS       DASWNJ010001338         2593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_621070, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001338 DASWNJ010000000
VERSION     DASWNJ010001338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621070"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..866
                     /gene="dapB"
                     /locus_tag="VGB85_22280"
     CDS             45..866
                     /gene="dapB"
                     /locus_tag="VGB85_22280"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEY0136833.1"
                     /translation="MSERLTPIVIVGARGRMGKALLREIIISDSMVLEAAVDRSGGPG
                     LGQDAGRLAGLHELGLVVTDELLPRRSSVVVDFSLAQATEANVRKCADAGVPLILGTT
                     GLQKTTRELLRAAAKDIPIVSAANFSVGVTLLTKIAGLVAQSLGEQWDAEILEIHHSH
                     KRDAPSGTALRIAKAVAKATGRDLETALRSDRADAHARPREAGEIGISAIRGGDSVGE
                     HTLMFLTDGERLELTHRATDRAIFARGALRAAQWVVDQPPGLYSMADVLGLSALG"
     gene            873..1220
                     /locus_tag="VGB85_22285"
     CDS             873..1220
                     /locus_tag="VGB85_22285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136834.1"
                     /translation="MTRLEFRLLARTRTDAGEHGSFAVQIPTDLLYLQGHFPDYPLLP
                     GVAQLLALVLDRTHALWPELDQPRRISRLKFRHGIFPNDELEVRLDRSGDEVRFALQR
                     GDQVCTSGALIFT"
     gene            1255..>2593
                     /locus_tag="VGB85_22290"
     CDS             1255..>2593
                     /locus_tag="VGB85_22290"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0136835.1"
                     /translation="MVQDPRTVDIAILGGGLAGNLLARQLRRQLPTLSVALFERTRET
                     SFKVGESTVEIASNYLTRRLGLSTYLYENQLPKNGLRFFFDTPQKDAALVDMSEIGSA
                     HLPKFPSFQIDRQRIEADLMRMNVADGVQLHTGARVHDLKLHEQASPNASGMHEFTVT
                     EGEAQYPVRARWVVDASGRASAIARLKDLRVPTPEHGIAAAWGRFTGHADMDDWGPPA
                     WRGRVHHTSRVLSTNHFCYPGYWIWFIPLGRGVMSVGWVGEHKIFRDSMRKPEGFLEF
                     LKGHRACWDLLKERAELIDVLAYKQLAYGTKQFFGGADRWFMVGEAAAFTDPFYSPGS
                     DFISMENDFITDMIARDHAGESRAAIAERSQTYDEFMQFRFQATMLLYRDLYPVLGSF
                     ELLKLKWDYDIACYYNLWAAPFWHDQHLDMRHLQGELRRKEFVLQGLSNFSNLF"
BASE COUNT          414 a          989 c          789 g          401 t
ORIGIN      
        1 caggccctcg ccgataccct gcaaaccctc ggactcgaca caccatgagc gaacgcctga
       61 cccccatcgt catcgtcggc gcccgcggcc gcatgggcaa agccctcctc cgcgagatca
      121 tcatcagcga cagcatggtg ctcgaggccg ccgtcgaccg ctccggcggc cccggcctcg
      181 gccaggacgc cggccgcctc gccggcctcc acgagctcgg cctcgtcgtc accgacgagc
      241 tgctcccacg ccgcagcagc gtcgtcgtcg acttctcgct cgcgcaggcg accgaggcca
      301 acgtgcgcaa gtgcgccgac gccggcgtcc cgctgatcct cggcaccacc ggcctgcaaa
      361 agaccacccg cgagctgctg cgcgccgccg ccaaggacat accgatcgtc agcgccgcca
      421 acttcagcgt cggcgtcacc ctgctgacca agatcgccgg cctcgtcgcg cagtcgctcg
      481 gcgagcagtg ggacgccgag atcctcgaga tccaccacag ccacaagcgc gacgccccgt
      541 cgggcaccgc cctgcggatc gccaaggccg tcgccaaggc gaccggacgc gacctcgaga
      601 ccgccctgcg cagcgatcgc gccgacgccc acgcccgccc gcgcgaggcc ggcgagatcg
      661 gcatctcggc gatccgcggc ggcgattcgg tcggcgagca caccctcatg ttcctgaccg
      721 acggcgagcg cctcgagctc acccatcgcg ccaccgaccg cgcgatcttc gctcgtggtg
      781 ccctgcgcgc cgcccagtgg gtcgtcgacc agcccccggg gctgtactcg atggcggacg
      841 tgctcggcct gtccgccctc ggctaagggc tcatgacgcg gctcgagttt cgcctgctcg
      901 ctcgcacccg cacggacgcg ggcgagcacg gcagcttcgc cgtgcagatc cccaccgacc
      961 tgctgtacct gcaaggtcac ttcccggact acccgctgct cccgggcgtc gcgcagctgc
     1021 tcgcgctcgt gctcgaccgc acccatgcgc tgtggcccga gctcgatcag ccccggcgga
     1081 tctcccgcct aaagtttcgt cacggcattt ttccaaacga cgagctcgag gttcgcctcg
     1141 atcgcagcgg tgacgaggtc cgcttcgcgc ttcagcgcgg ggaccaggtg tgcaccagcg
     1201 gcgcgctgat cttcacctga gggatctccc tgggatcggc tatcttcgcc gtccatggtc
     1261 caagacccgc gcaccgtcga cattgcgatc ctcggcggcg gcctggccgg caacttgctg
     1321 gcccggcagc tgcgccggca gctcccgaca ctgtcggtcg cgctgttcga gcgcacccgc
     1381 gagaccagct tcaaggtcgg cgagtcgacc gtcgagatcg ccagcaacta cctgacgcgt
     1441 cggctcggcc tctcgaccta tctctacgag aaccagctgc ccaaaaacgg cctgcgcttc
     1501 ttcttcgaca cgccgcaaaa agacgccgcg ctggtcgaca tgagcgagat cggcagcgct
     1561 cacttaccca agttcccgag cttccagatc gatcgccagc gcatcgaggc cgacctgatg
     1621 cgcatgaacg tcgccgacgg cgtgcagctg cacaccggcg cccgcgtgca tgacctgaag
     1681 ctgcacgagc aggccagccc gaacgcgagc ggcatgcacg agttcaccgt caccgagggc
     1741 gaggcgcagt accccgtgcg cgcgcgctgg gtcgtcgacg ccagcggccg cgccagcgcg
     1801 atcgcccgcc tcaaggacct gcgcgtgccg accccggagc acggcatcgc ggccgcctgg
     1861 ggccgcttca ccggccacgc cgacatggac gactggggcc cgccggcgtg gcgcggccgc
     1921 gtgcaccaca cctcgcgtgt cctctcgacc aaccacttct gctaccccgg ctactggatc
     1981 tggtttatcc cgctcggccg cggcgtgatg tcggtcggct gggtcggcga gcacaagatc
     2041 tttcgcgact cgatgcgcaa gcccgagggc ttcctggaat tcctcaaggg ccaccgcgcc
     2101 tgctgggacc tgctcaagga gcgcgccgag ctgatcgacg tgctcgccta caagcagctc
     2161 gcgtacggca cgaagcagtt cttcggcggc gccgaccgct ggttcatggt cggcgaggcg
     2221 gcggccttca ccgacccctt ctacagcccc ggcagcgact tcatctcgat ggagaacgac
     2281 ttcatcaccg acatgatcgc gcgcgatcac gctggtgagt cgcgcgccgc gatcgccgag
     2341 cgctcgcaga cctacgacga gttcatgcag tttcgcttcc aggcgacgat gctgctgtac
     2401 cgcgacctct acccggtgct cggcagcttc gagttattaa aactcaagtg ggactacgac
     2461 atcgcctgct actacaacct gtgggccgcg ccgttttggc acgaccagca cctcgacatg
     2521 cgccacctcc agggcgagct gcgccgcaag gagttcgtgc tgcagggttt gagcaatttt
     2581 tcgaatctgt tcc
//
LOCUS       DASWNJ010001339         5179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_59986, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001339 DASWNJ010000000
VERSION     DASWNJ010001339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59986"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VGB85_22295"
     CDS             <1..201
                     /locus_tag="VGB85_22295"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEY0136836.1"
                     /translation="LVALGVPIDLNRASADELEGLPGIGPTLARRIIDARPLRRVDDL
                     VRVSGIGPVRLARLRPLVTTGL"
     gene            289..2109
                     /locus_tag="VGB85_22300"
     CDS             289..2109
                     /locus_tag="VGB85_22300"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0136837.1"
                     /translation="MNSKRLPRYLGLGLTTLVLAACGDDGNTTVTAGSQTDSATSDDA
                     TDSAPTEATTQTSQTSQTSKTTDDPTGGSDSLTGGDTGSSTVASDGDTDDTAAETANV
                     SATDTATETATDTAPDTTTGDVTPECGNGKVEGTEECDDGNDEPADGCEPGCQNTPVC
                     GNSEIEVGESCDDGNTVDGDECSADCQTVTTPIVCGDGKQEGDEQCDNGPDVPDDGCE
                     ADCTISPPECGNGKVEEGEACDDGNLVNGGPNDFCKNDCSVFTPASCQAPAEYTICDD
                     AINLGDKNDKTNAHKAMGICNADANNSVQITNFDFKSNNNAAWQVAKGFGTYTYDHDM
                     DANTPNQLFYSPREGSTFLMVSSGVIKAPNNNGIVVESNNSQGGNGDNGNDDGNQLPA
                     PFQSKVGSANGAGGTPFQNCDGVKDCSDTLQAQWTKGNSNPNDKLWFTFKTKVPLGTF
                     GYSFDFVFCSSEWPVYVNSGFNDLLIAYQVDPSADDPNADPPIDPYTGNVTFIPDPND
                     AKKGLPLTITALDPYFDGPGFTYNEEPLKGTGFEQHACTEWFTAKGGVQPGAEVTIGF
                     FIADMSDNILATLAILDNFRWDCEGCIPSEVDDCGVQDPQ"
     gene            2331..4130
                     /locus_tag="VGB85_22305"
     CDS             2331..4130
                     /locus_tag="VGB85_22305"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0136838.1"
                     /translation="MTLQRSIFRSLGLGLGAVVLVACGDDGATTDAGTGTGTTTDQAT
                     TDPPTTTPTSTPTTTMSTQAGVTEGGTEGATGDDTGQTTADTGDTSGTGEPGDTTTAT
                     STGALSDSDTEPQAECGNGVPDVGEACDDGNDVPGDGCEPSCVLTPECGNGVPEEGEA
                     CDDGNTMDGDECSADCQTATPPPVCGDGEVQDPETCDDGNMDAGDGCEPDCTITPAEC
                     GNGKLEEGEACDDGNNVNGGPGDFCKNDCSVFVPATCNAPAEYTVCDNALDLANKADK
                     TQAHKAIGICNAEAANSVQITDWSFNANDNASWQIAKGFGTYTYDHDMMPATPPRLLY
                     SPREGDTMLIVSTGTVTAPNNQGVVVQPPNSQAGFVGNNTNPDLPDTMPAPMVAKNGS
                     NNGAGGTPFQNCDGINDCSDTLQAQWFDIADADPNDKLWFTFKTKVPAGTFGYTFDFV
                     FCSGEWPVFVNSSFNDLFVAWQVDPTPDDPNANPPVDPYTGNVTFIPSPNEPDKGLPL
                     TITALDPYYDGPGYTYAEPQLQGTGFEQHACSDWFTAKGGVQPGADLTIAFFITDMGD
                     SSYTSTALIDNFRWDCKGCVPSEVDDCGVQPPM"
     gene            4479..>5179
                     /locus_tag="VGB85_22310"
     CDS             4479..>5179
                     /locus_tag="VGB85_22310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0136839.1"
                     /translation="MKTRSISRIFTLGLSAAALAACGENSGNTTSDATDSASSEASTT
                     NGPTTPTSTTNMTQVSVTEGDTTVEPTGGSGTATGDESSGTATITDGTSNSSSEGSAT
                     TSTTDPVAECGDGVVEGTEECDGGEGCEADCTLTPVCGNGNVEGGEVCDDGNTDDGDE
                     CSADCQTASPPAVCGDGTVQDPEQCDNGPDVPEDGCEDDCTLTPVECGNGIKEADEQC
                     DDGNKTNGGPGDFCK"
BASE COUNT         1025 a         1835 c         1683 g          636 t
ORIGIN      
        1 ctggtcgccc tcggcgtccc gatcgacctc aaccgcgcct ccgccgacga gctcgaggga
       61 ttaccgggga tcgggcccac gctggcccgg cgcatcatcg acgccagacc gctgcgccgg
      121 gtcgacgacc tcgtacgggt cagcggcatc ggtccggtcc ggctcgcacg cctgcgaccc
      181 ctcgtgacca ccggcctgtg acagcggcga cgaggccgtg agtctcatct cgatactcag
      241 ctcatgcacg cccgctttta gcggagggtg tgcggtaata cacggaatat gaactcgaag
      301 agactgcccc gatatctcgg ccttggcctg acgactctgg tgctcgctgc gtgtggtgat
      361 gacggcaata cgaccgtgac cgccggcagc cagaccgaca gcgcgacctc ggacgacgcc
      421 accgacagcg cgccgaccga ggcgacgacg cagaccagcc agaccagcca gacctcgaag
      481 acgaccgacg atccgaccgg cggcagcgat tcgctcaccg gcggggacac cggcagcagc
      541 acggtggcga gcgacggtga caccgacgac accgcggccg agacggcgaa cgtcagcgcg
      601 acggacacgg cgacggagac ggcgacggac accgctccgg acacgacgac cggggacgtg
      661 acgccggagt gcggcaacgg caaggtcgag ggcaccgagg agtgtgacga cggcaacgac
      721 gagcccgcgg acggctgcga gccgggctgc cagaacacgc ccgtgtgtgg caacagcgag
      781 atcgaggtcg gcgagtcgtg cgacgacggc aacaccgtcg acggcgacga gtgcagcgcc
      841 gactgccaga cggtgaccac gccgatcgtc tgcggcgacg gcaagcagga gggcgacgag
      901 cagtgtgaca acggccccga cgtgcccgat gacggctgcg aggccgactg cacgatcagc
      961 ccgccggagt gcggcaacgg caaggtcgag gagggcgagg cctgcgacga cggcaacctg
     1021 gtcaacggcg ggccgaacga cttctgcaag aacgactgca gcgtgttcac cccggcgtcg
     1081 tgtcaggcgc cggccgagta cacgatctgc gacgacgcga tcaacctcgg cgacaagaac
     1141 gacaagacca acgcgcacaa ggcgatgggc atctgcaacg ccgacgccaa caactcggtg
     1201 cagatcacca acttcgactt caagagcaac aacaacgccg cgtggcaggt ggccaagggc
     1261 ttcggcacct acacttacga ccacgacatg gacgccaaca cccccaacca gctgttctac
     1321 agcccgcgcg agggtagcac cttcctgatg gtcagctccg gcgtcatcaa ggcgccgaac
     1381 aacaacggca tcgtcgtcga gagcaacaac tcgcagggcg gcaacggtga caacggcaac
     1441 gacgacggca accagctgcc cgcgccgttc cagtccaagg tcggctcggc caacggcgcc
     1501 ggcggcacgc cgttccagaa ctgcgacggg gtcaaggact gctccgacac gttgcaggcc
     1561 cagtggacca agggcaacag caacccgaac gacaagctgt ggttcacctt caagaccaag
     1621 gtcccgctcg gcacctttgg ttacagcttc gacttcgtgt tctgctcgtc cgagtggccg
     1681 gtctacgtca attccggctt caacgacctg ctgatcgcct accaggtcga cccgtcggcc
     1741 gatgacccga acgccgaccc gccgatcgac ccgtacaccg gcaacgtcac cttcatcccg
     1801 gacccgaacg acgcgaagaa gggcctgccg ctgacgatca cggcgctcga cccgtacttc
     1861 gacggcccgg gcttcaccta caacgaggag ccgctgaagg gcaccggctt cgagcagcac
     1921 gcgtgcaccg agtggttcac cgccaagggc ggcgtccagc cgggggccga ggtcacgatc
     1981 ggcttcttca tcgccgacat gagcgacaac atcctcgcca ccctggcgat cctcgacaac
     2041 ttccgctggg actgcgaggg ttgcatcccg agcgaggtcg acgactgcgg cgtccaggac
     2101 ccgcagtaat ctcgtgatca cctgaccggc gctcacgcgc cgctcggaga acggcaggcg
     2161 tgcatgcacg gcctgccgtt cgcaattccg cgttctcgta ttcgcctcgc ggtccatgcg
     2221 caacccggcg cagcccgggc cggcgcccga tccaccggca gcaagccccg catcgaccac
     2281 gaatgcgggc gtcgggcggc gccgcgcagc ggtccgggac aagaacgccc atgaccttgc
     2341 aacgaagcat ctttcgatcc ctcggcctgg gcctcggcgc cgtggtcctc gtcgcctgtg
     2401 gagacgacgg cgcgaccacc gatgcgggca cgggcactgg cacgaccacc gaccaggcga
     2461 ccaccgaccc gccgacgacc acgccgacga gcaccccgac gacgacgatg agcacgcagg
     2521 ccggcgtgac cgagggcggc accgaggggg cgactggcga cgacacgggc cagaccaccg
     2581 cggacacggg cgacacgagc ggcactggcg agccgggcga cacgaccacg gccacctcga
     2641 ccggggcgct cagcgacagc gacaccgagc cgcaggcgga gtgcggcaac ggcgtgcccg
     2701 acgtgggcga ggcctgcgac gacggcaacg acgtgcccgg cgacggctgc gagccgagct
     2761 gcgtattgac gccggagtgt ggcaacggcg tgcccgagga gggcgaggcc tgcgacgacg
     2821 gcaacacgat ggacggcgac gagtgcagcg ccgactgtca gacagcgacc ccgccgccgg
     2881 tgtgcggcga cggtgaggtt caggacccgg agacctgcga cgacggcaac atggacgccg
     2941 gcgacggctg cgagccagac tgcacgatca ctccggcgga gtgcggcaac ggcaagctcg
     3001 aggagggcga ggcctgcgac gacggcaaca acgtcaacgg cggccccggc gacttctgca
     3061 agaacgactg cagcgtgttc gtgccggcga cctgcaacgc gccggccgag tacaccgtct
     3121 gcgataacgc gctcgacctc gccaacaagg ccgacaagac ccaggcccac aaggcgatcg
     3181 gcatctgcaa cgcggaggcg gccaactcgg tgcagatcac cgactggagc ttcaacgcga
     3241 acgacaacgc ctcgtggcag atcgccaagg gcttcggcac ctacacctac gatcacgaca
     3301 tgatgccggc caccccgccc aggctgctgt acagcccgcg cgagggtgac acgatgctga
     3361 tcgtcagcac cggcacggtc accgcgccga acaatcaagg ggtcgtcgtg cagccgccga
     3421 actcgcaggc gggcttcgtc ggcaacaaca ccaaccccga cctgcccgac acgatgcccg
     3481 cgccgatggt cgccaagaac ggcagcaaca acggcgccgg cggcacgccg ttccagaact
     3541 gcgacgggat caacgactgc tcggacacct tgcaggcgca gtggttcgac atcgccgacg
     3601 ccgacccgaa cgacaagctg tggttcacct tcaagaccaa ggtccccgcc ggcaccttcg
     3661 gctacacctt cgacttcgtg ttctgctccg gcgagtggcc ggtcttcgtc aacagctcct
     3721 tcaacgacct gttcgtcgcc tggcaggtcg acccgacccc cgacgacccg aacgcgaacc
     3781 cgccggtcga cccgtacacc ggcaacgtca cctttatccc ctcgccgaac gagccggaca
     3841 agggcttgcc gctgacgatc accgcgctcg acccgtacta cgacggcccg ggctacacct
     3901 acgccgagcc gcagctgcag ggcaccggct tcgagcagca cgcctgctcc gactggttca
     3961 ccgccaaggg cggcgtgcag ccgggcgccg atctgacgat cgcattcttc atcaccgaca
     4021 tgggtgactc gtcctacacg agcaccgcgc tgatcgacaa cttccgctgg gactgcaagg
     4081 gttgtgtgcc gagcgaggtc gacgactgcg gcgtgcaacc gccgatgtga tcgaccgatc
     4141 gcgtcgcctg caagatcgaa cgacaggtcg tgcatcgcac gacctgtcgt cgtcgtgcgc
     4201 gccacgagcg gcgacttccc gttccatggt cgtggccggc gccgtataca tcttcattgc
     4261 gctgtcacga agccgcgccg atcctcgcgc tacgtcgcgc gccggctcca gttcgcgccg
     4321 cacgaccacg cgttcggctt gaaaacaatc gcggtcaagt gaccgctgtg cccgggggcg
     4381 gcgcgctgca tcgcctcttg gaaccaccgc gccgtcgtat tagcctgggc tcgcccctcg
     4441 ggctgcgcgt gctgttcgac gagggccgca ggattatcat gaaaactcga agcatctctc
     4501 gaatcttcac ccttgggctc agcgccgccg ccctcgctgc atgcggcgaa aacagtggga
     4561 ataccacctc ggacgcgacc gacagcgcgt cgtccgaggc cagcacgacc aacggaccga
     4621 ccaccccgac gtcgaccacc aacatgacgc aggtcagcgt cacggaaggc gacaccaccg
     4681 tggagccgac cggtggttcg ggcaccgcga cgggcgacga gagcagcggc accgcgacga
     4741 ttacggacgg cacgagcaac tcgtccagtg agggcagcgc gaccaccagc accaccgacc
     4801 cggtcgcgga gtgtggcgac ggcgtcgtcg agggcaccga ggagtgcgac ggtggcgagg
     4861 gttgcgaggc cgactgtact ttgacgccgg tgtgcggcaa cggcaacgtc gagggcggcg
     4921 aggtctgcga cgacggcaac accgacgacg gcgacgagtg cagcgccgac tgtcagactg
     4981 ccagcccgcc ggcggtctgc ggcgacggca cggtccagga ccccgagcag tgcgacaacg
     5041 gccccgacgt ccccgaggac ggctgcgagg acgactgcac cctgaccccg gtcgagtgcg
     5101 gcaacggcat caaggaggcc gacgagcagt gcgacgacgg caacaagacc aacggcggcc
     5161 ccggcgactt ctgcaagaa
//
LOCUS       DASWNJ010001340         2301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_128836, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001340 DASWNJ010000000
VERSION     DASWNJ010001340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128836"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VGB85_22315"
     CDS             complement(<1..187)
                     /locus_tag="VGB85_22315"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HEY0136840.1"
                     /translation="MQNASTLPASLAGMNLEKLGIELPIEGMTCASCVSRVEKALAKV
                     PGVERASVNLATESASVS"
     gene            356..550
                     /locus_tag="VGB85_22320"
     CDS             356..550
                     /locus_tag="VGB85_22320"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEY0136841.1"
                     /translation="MIELNLPDMTCGHCASTVAKTVAFVDPTAKVEVDLDSKKVKIQS
                     TEDRQDFAEALTDAGYPPAQ"
     gene            complement(919..1629)
                     /locus_tag="VGB85_22325"
     CDS             complement(919..1629)
                     /locus_tag="VGB85_22325"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEY0136842.1"
                     /translation="MTPADHKPLAGALLAEFLGTAGLLCAVIGSGIMAEKLSDGNDGV
                     ALIANTLATVFALFVLIEVFGPVSGAHFNPAVTLALAIRGLFRPPEKAAVPLYVAAQL
                     AGAAVGAWVAHAMFDLPILQLSSKARGGGGQLLGEFVATAGLMLVILRAPAGKGSALV
                     ACYIGAAYWFTSSTSFANPAAVFGRLLSDTFAGIAPANAPGFVVAQLLGAAAGCGLAV
                     LLERSGKPRPPASESGSS"
     gene            complement(1626..2051)
                     /gene="arsC"
                     /locus_tag="VGB85_22330"
     CDS             complement(1626..2051)
                     /gene="arsC"
                     /locus_tag="VGB85_22330"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065799.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEY0136843.1"
                     /translation="MTDVTIYHNPDCGTSRNTLAILRHAGVEPNVVEYLKTPPSKEEL
                     RKLLADAGLSVRDALREKGTPYGELNLADPKWTDEELLDFVGKHPILMNRPLVVTPLG
                     AKLCRPSEVVLEILPVGKMPDHVKEDGERVVDSGQRRSK"
     gene            complement(2072..>2301)
                     /locus_tag="VGB85_22335"
     CDS             complement(2072..>2301)
                     /locus_tag="VGB85_22335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEY0136844.1"
                     /translation="QAAGELCPVWPGQPVSAHWGMPDPGAVEGSEETRLKAFRDVAVM
                     MKRRIDLLLVMPLEKLDRLTIEKQARDIGRA"
BASE COUNT          372 a          785 c          761 g          383 t
ORIGIN      
        1 ccgagacgct cgcgctctcg gtggcgaggt tcacgctggc gcgctcgacg ccggggactt
       61 tcgcgagcgc cttctcgacg cgcgacacgc acgaggcgca ggtcatgccc tcgatcggca
      121 gctcgatgcc cagcttctct aggttcatgc cagcgaggct ggcaggcaat gttgacgcgt
      181 tctgcatggt tggctccttg cgcccgaaaa ccaggcgatg gaggaatgat caacgttgcc
      241 atcatggcaa ggtcaagccc ctaccgcaag aaagtttttc gcagctccct gttgactctg
      301 acacggtgac aaccttgacg atgcagtcct tcactcactt ttcaaggagt cagccatgat
      361 cgaactcaac ctcccggaca tgacctgcgg ccactgcgcg agcaccgtcg ccaagacggt
      421 cgctttcgtc gaccccaccg ccaaagtcga ggtcgacctg gacagcaaaa aggtcaagat
      481 ccagtcgacg gaggaccggc aggacttcgc cgaagcgctg accgacgccg gctacccgcc
      541 ggcccagtga cgcgggagcc gcggcgatgg acctgggtta tctgccgccc ctccccgtgg
      601 ggctgatgtc agctgcggcg cgcgacctgg ccgggcgcgt tggacctgtc gccgcggccg
      661 ccgagcgccg actgcctagc cggccagcgg ccgtcgttct cgcctcggtg tgcgccgcgt
      721 ggctaatcaa cgctatggat tggggctaca gcgacgtccg tttcctagtc gccatgagcc
      781 tgggcagcct ggtggccacc tccgccctcg catttggttg gtcgtccgag cctgaggagc
      841 tcgagcgatg gtctgcgagg gctctgctcg ccgcggtcgc gtccgcgctc ccagcgctta
      901 gctgggcttg acccccggct aagaggagcc tgactcggag gccggcggcc ttggcttacc
      961 cgagcgctcc aggaggaccg ccaagccgca cccggcggcg gcgcccagga gctgcgccac
     1021 gacaaatccg ggcgcgttcg ccggcgcgat tcccgcgaac gtgtcggaaa gcaggcgccc
     1081 gaagacagcg gcgggattcg cgaacgaggt gctggaagtg aaccagtagg ccgccccgat
     1141 gtagcaggcg accagggccg agccctttcc ggctggagcc cgcaaaatca ccaacatcaa
     1201 gcccgcggtg gcgacgaact cgcccaggag ttggcccccg ccgccgcgcg ccttcgagct
     1261 cagttggagg atggggaggt cgaacatcgc gtgggcgacc caagctccga ctgccgcccc
     1321 cgcgagctgg gccgcaacgt atagaggcac ggccgctttc tcgggaggac ggaacaggcc
     1381 tcttatggct aaggccagcg tcaccgccgg attgaagtgc gcgccgctga cgggtccgaa
     1441 gacctcgatc aggacgaaga gcgcgaagac ggtggcgagc gtgttggcga tgagcgccac
     1501 cccgtcgttt ccgtcggaga gcttctcggc catgatccct gagccgatca ccgcgcacag
     1561 cagccccgcg gtgccaagga actccgcgag cagggctccc gcgagcggct tgtggtccgc
     1621 cggggtcact tcgagcgccg ctggcccgag tcgacgacgc gctcgccgtc ctctttcacg
     1681 tggtcgggca tcttgcccac cggcaggatc tcgaggacca cctccgaggg cctgcaaagc
     1741 ttggcgccca acggcgtgac caccagcggc cgattcatca ggatcggatg cttgccgacg
     1801 aagtccagca gctcctcgtc ggtccacttc gggtcggcca gattgagctc gccgtagggc
     1861 gtgcccttct cgcgcagcgc gtcgcggacc gagagccccg cgtcggccag gagcttgcgc
     1921 agctcctcct tcgaaggagg cgtcttcaag tattcgacga cgttaggctc gacgccggcg
     1981 tggcgcagga tggccagcgt gttgcgcgag gtgccgcagt cggggttgtg atagatcgtg
     2041 acgtccgtca tgtgggcctc ttggtttgtg ttcatgcgcg cccgatgtcg cgggcctgct
     2101 tctcgatggt cagccggtcg agcttttcga gcggcatgac caagagcagg tcgatgcgcc
     2161 gcttcatcat caccgcgacg tcgcgaaacg ctttgaggcg cgtctcctcg ctgccttcca
     2221 ccgccccggg gtcgggcatg ccccagtgcg cgctcaccgg ctgccccggc cacaccgggc
     2281 acagctcgcc ggcagcctgg t
//
LOCUS       DASWNJ010001341         2573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_128873, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001341 DASWNJ010000000
VERSION     DASWNJ010001341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128873"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..1515
                     /locus_tag="VGB85_22340"
     CDS             184..1515
                     /locus_tag="VGB85_22340"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEY0136845.1"
                     /translation="MAIFGGTEVEACGWPTVVGVSGGGFECTGTLVHPRVVMFAAHCG
                     DGLKKIHFGEDLDAPATTIQAEMCQVNPDYGGPQDQEHDWGFCRLGAPITELPVTPVV
                     FGCERDLVVPKDMATIAGYGYISDDSAAGRKHWAAAPIRQVYSQTADVGGLGDPGVCA
                     GDSGGAAFVRYPDGSWHTFGIASTVGGSCGGVGTYALAWNAVPFIEENAGIDITPCHD
                     ADGTWNPSFRCGDFFAGEPGAGAGDWPGWCAGSPAIAASTTCGAAFDAEPDDDPPSVE
                     ITTPTSGEHPQNGFSTAIEIAADDGEGWGIAAVRIKINGMVQPVADDEPPYSFAKVNF
                     PAGTWELIAVAEDAAGHVAESAPVTLVVGSDAPATGGEDTTPTSSSAGSDDTGASDTG
                     DTATMNAGEDGCGCASGDPAAGALLLGLLGFRRKRPATTTTSGSVPRSSRA"
     gene            1594..1914
                     /locus_tag="VGB85_22345"
     CDS             1594..1914
                     /locus_tag="VGB85_22345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136846.1"
                     /translation="MSLAPLLLLASLSLMFAGLVLLIEREHDELSELCPAELSTSAPV
                     PRSCWERIQERVRHWRAPQPRRRRRSGGPRQVVLEMAEISAARRLFCGRVADLGSRPG
                     AWES"
     gene            complement(1917..2378)
                     /locus_tag="VGB85_22350"
     CDS             complement(1917..2378)
                     /locus_tag="VGB85_22350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136847.1"
                     /translation="MPSRRLLLSCLLLAACPPRTPPEPPPVDAANAGDGGSAAEEGQP
                     PPDSDVAPLAPDDPDAPDEPGEVEPGDKPGNKPGKPGKPGKPGSMRGDNLGDTLSTRA
                     SRGSSRKSSGGCDGGARRIGETWKIDCNSCSCGDDGQSTCTELACGSEPAR"
BASE COUNT          329 a          904 c          985 g          355 t
ORIGIN      
        1 gcagcgtcag cattttggcc aggggcgccc gaccggatgt gtcatcctcg ggcgatgcgc
       61 gcgtacctgt gggtgcttcc ggcctgtctg gtccaactgg ccgcgggagg gctctgcgcg
      121 gccgctgcga ggccgggcgg cgccgggagc ccggtcggga tctccgcggg cggacccggg
      181 ccggtggcca tcttcggcgg cacggaggtc gaggcgtgcg gctggccgac ggtggtcggg
      241 gtctcgggcg ggggcttcga atgtaccggc acgctggtcc acccgcgcgt cgtgatgttc
      301 gccgcacact gcggcgatgg cctgaagaag atccacttcg gggaagatct ggacgcgccc
      361 gcgaccacga tccaggccga gatgtgccag gtcaaccccg actacggcgg gccgcaggac
      421 caggagcacg actggggctt ttgtcggctc ggcgcgccga tcaccgagct gccggtcacg
      481 ccggtggtgt tcggctgcga gcgcgacctc gtcgtgccca aggacatggc gacgatcgcc
      541 ggctacggtt acatcagcga cgacagcgcg gccggccgca agcactgggc ggcggcgccg
      601 atccggcagg tctactcgca gacggcggac gtcggcggcc tcggcgatcc gggcgtgtgc
      661 gccggcgact ccggcggcgc ggccttcgtg cgctacccgg acggcagctg gcacaccttc
      721 ggcatcgcct cgacggtcgg tggctcgtgc ggcggcgtcg gcacctacgc gctcgcctgg
      781 aacgcggtgc ccttcatcga ggagaacgcg ggcatcgaca tcacgccgtg ccacgacgcg
      841 gacgggacct ggaacccgag ctttcgctgc ggcgacttct tcgccggcga gccgggggcc
      901 ggcgccggcg actggcccgg gtggtgcgcg ggcagcccgg cgatcgcggc gagcaccacc
      961 tgcggcgcgg ccttcgacgc cgagcccgac gacgatccgc cgagcgtcga gatcacgacg
     1021 ccgacctccg gcgagcaccc acaaaacggc ttcagcaccg cgatcgagat cgccgcggac
     1081 gacggcgagg gctgggggat cgccgcggtg cggatcaaga tcaacggcat ggtccagccc
     1141 gtcgccgacg acgagccgcc gtacagcttc gccaaggtca acttccccgc ggggacgtgg
     1201 gagctgatcg cggtcgccga ggacgccgcg ggacacgtcg ccgagtcggc gccggtcacc
     1261 ctcgtcgtcg gctcggacgc gcctgcgacg ggcggcgagg acacgacgcc gacgtcctcg
     1321 agcgccggca gcgacgacac gggcgcgagc gacacgggcg acaccgcgac gatgaacgca
     1381 ggcgaggacg gctgcggttg tgccagtggc gatcccgcgg ccggtgcgct gctgctgggt
     1441 ctgctgggtt tccggcgaaa acggccggcc acgacaacga cgtccggatc ggtgccgcgt
     1501 tcgtcgcggg cttgacactg aattctcgga gggtttcgcg gcttcgcggc tggcctgggg
     1561 cttgctctac ctggagtgtc ccacgaggtc accatgtcgc tcgctcccct cctgctgctc
     1621 gcttcgctct cgctgatgtt cgccggcctc gtgctgctga tcgagcgcga gcacgacgag
     1681 ctgagcgagc tctgcccggc cgagctcagc accagcgcgc cggtgccccg cagctgctgg
     1741 gagcggatcc aggagcgcgt gcggcactgg cgagcgccgc agccgcgccg gcgccggcgg
     1801 tcgggtggcc cgcgccaggt cgtgctcgag atggcggaga tcagcgccgc gcgccgcctg
     1861 ttctgcgggc gcgtcgcgga cctcggcagc cgtccgggcg cctgggagag ctgagctcag
     1921 cgcgccggct cggacccgca ggccagctcg gtgcacgtgc tctggccgtc gtccccgcag
     1981 ctgcacgagt tgcagtcgat cttccaggtc tcaccgatcc ggcgcgcgcc gccgtcgcag
     2041 ccgcccgagg acttgcgtga ggagccccgg ctcgcgcgcg tcgagagggt gtccccgaga
     2101 ttgtcgccgc gcatgctacc gggcttaccg ggcttgccgg gcttgcccgg cttgttgccc
     2161 ggcttgtcgc cgggctcgac ctcgccgggc tcgtccgggg cgtccgggtc gtccggcgcg
     2221 aggggcgcga catcgctgtc ggggggcggc tggccctcct cggccgcgga gccgccgtcg
     2281 cccgcgttcg cggcatcgac gggcggcggc tccgggggcg tccgaggcgg gcaggcggcc
     2341 agcagcaggc acgagagcag caggcggcgc gagggcatgg ccgcaaggta gcacgggtgc
     2401 ggcgcgcgtt cggtgcgggg gattacacga cgcgcaggcg gcgatccacg ccacgtcact
     2461 ggcagacgat cgccggcgcc gggcccggag tctgccagtg ccgccggcgc gcgatcgtct
     2521 ggccccgagg acatgtcgca taggacatgt ccgtcgggac atcccatgca gac
//
LOCUS       DASWNJ010001342        10529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_445778, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001342 DASWNJ010000000
VERSION     DASWNJ010001342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 10529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10529
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445778"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VGB85_22355"
     CDS             <1..357
                     /locus_tag="VGB85_22355"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY0136848.1"
                     /translation="WAARAPLLLILIARRNFTHNNKPNRNAAFDAGAAWMSLALQAER
                     LGLHTHAMAGFDTARAYSELGVDPEAYEALAAIAVGRRDEPETLPEPLRAREVPSGRN
                     PQASFVFHGRFPAPTP"
     gene            435..1250
                     /locus_tag="VGB85_22360"
     CDS             435..1250
                     /locus_tag="VGB85_22360"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY0136849.1"
                     /translation="MSWSWNIGRLFGIDTRVHASFVLLLLWAAFSSYTSAGTVTAAVM
                     GVVFMIAVFASVVAHELGHALTARVHGIETRQILLLPIGGVAQLEHGHMRPGVELQVA
                     LAGPVVSFLLAGVLFTFAAVAGDVSPDSFIGSLAWTNLGLAVFNMLPAFPMDGGRVLR
                     AALSSRMGHLRATDIAVTLGKAGAVLFLVLGLFTGRFMLMLLAAFLYFAARSEPRVLP
                     HPGPRRSQGIHNIMGAPGYDPALLRDGDDPDLVLARSPRGRLWVLRRNDPRWN"
     gene            1368..2840
                     /locus_tag="VGB85_22365"
     CDS             1368..2840
                     /locus_tag="VGB85_22365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HEY0136850.1"
                     /translation="MSPHRLLALLLPLAACPSGADETSLTASTGPGTTGAPDTGSSPT
                     TGDTTDVPTTATATSEPTTATATTDPSTTDAPTTDTTDDTTTDAPPAMPTVLLPRASI
                     TNTELAVLVNDQDPQSMAVAAYYQQARQIPAQNVITVSFPPTETLATDAFNSLKAQID
                     AAAGPQIQAFAVAWTNPHKVDCQSLVSALALGADMKYCQPQPPCNPTAPGGFYDSASL
                     APFTDLGIRPAMHLAGVTADDVFKLIDRGVAADNTYPSATGYLLRTTDVARSVRFFQF
                     EAAKAAWAYDGGLALEYIDNSGGMGLDYIEGKTDVLFYLTGLTEVPQIASNMYLPGAI
                     ADHLTSIGGHIPENAGQMSIVRWLEAGATASYGTAFEPCNYTNKFPDAGVLLPHYFRG
                     ETLIEAYWKSVDWPGEGVFVGEPLARPWGGPIFTWEPPMLTVETYQLVPGKLYTLESG
                     PGVDGPWTLVQDDIMTTQNKRTAITIADASEPFYRLTVKP"
     gene            2866..3375
                     /locus_tag="VGB85_22370"
     CDS             2866..3375
                     /locus_tag="VGB85_22370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136851.1"
                     /translation="MAWPRAITLALLSLVGCTSEPDAPTTQDTSSSTGVTTGSTGATG
                     TTTGTTGEPTSGTTAADGCASHPPGDWAACQKGGLTDNSLCGWMEGSGNGMITCLSPQ
                     SGSFNVCGIRDCVDDCDCFAAPLTGDAVPSCRVVFGGGGTACVLYCANGQTCPDGMEC
                     SSGYCYWPN"
     gene            3382..4632
                     /locus_tag="VGB85_22375"
     CDS             3382..4632
                     /locus_tag="VGB85_22375"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0136852.1"
                     /translation="MRLAYLSHYFPPETNAPAARVHELSRALVRLGHEVHVVTGQPNH
                     PRGVVFPGYRAAEYRREVVDGIVVHRVPTFPAANRGVLLRGLGYAAVPTAQVALGLLK
                     IPRVDLVLATSPQIFTGVAGLALARLRRVPFVLEVRDLWPESIVSVGALPARHPVIRA
                     LERVEHHLYRSAAGIVSVTDSFVEHFVAHGVARSRIAVVKNGADLDLFDPDTPPTPLR
                     ARLGIPTDALLVMFCGTIGMAHDVGLLARAAAIVPDDLPVHFVIVGDGAGREALAAEI
                     AALGVGDRVHLLPAVPRSAVPGLLAAADLSAIVLRDSPTFAGVLPSKIFEAMAMRVPI
                     LLGVRGEARALVERAGAGLPFEPGDPRDLVATLRAFAMLGAPGRRAMGDAARAHALTD
                     HDRTRQAQQLAVFLERLGGAGRSQ"
     gene            4559..5899
                     /gene="egtB"
                     /locus_tag="VGB85_22380"
     CDS             4559..5899
                     /gene="egtB"
                     /locus_tag="VGB85_22380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEY0136853.1"
                     /translation="MTAPAKPSSSPSSSSDSAEPADHSELDAIRAASLALVASLSAED
                     MLAQSMPDASPVKWHLAHTTWFFETFVLAELPEHRPFSPSYASIFNSYYNSVGAPHPR
                     AQRGLLTRPDLAEVLRYRQHVDHALRRALTQGLLSPELRARVQVGLQHEQQHQELILT
                     DVQHLLFQNPLRPAFRRPTDDMSFGTAALSTSSASSTSSTSSASSASSASSAAPDAWH
                     SYPGGVVQVGHRGDDFCFDNELPRHRILLAPFEVATRPVSNAAYLQFIRDGGYRRPEL
                     WLSDGWDAVHRRGWFAPLYWSDDLATRFTLHGPRPIDPWAPVCHVSYYEADAYARWAG
                     ARLPTEHEWESAAEGLPVTGNFVEAGRLEPVHAHTPHFFGDVWVWTASPYVAYPGFKP
                     AAGALGEYNGKFMCNQIVLRGGSCATPRTHIRASYRNFFPPDARWQFSGLRLAR"
     gene            5902..6903
                     /gene="egtD"
                     /locus_tag="VGB85_22385"
     CDS             5902..6903
                     /gene="egtD"
                     /locus_tag="VGB85_22385"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEY0136854.1"
                     /translation="MLEVQRRGPRVPSIPVGKALPLLDLAPEAGDLRRDALAGLAASP
                     KTLPCKYFYDDHGSWLFDQICSLPEYYPTRTELAISHQYAAEMAAACGPQVLLLELGS
                     GSSTKTRVLLDHLRAPAGYVPVDIAREFLRSAADDLASAYAGLPVLPVCADFTRPFTA
                     PVLPARRTVVYFPGSTIGNFTAGEATLLLRNIGRLMGDAGGLLIGVDLRKDTAVLERA
                     YNDARGVTAAFNLNLLTRLNRELAADFDLTQFQHRAIWDPGPGRIEMQLVSTRAQTVH
                     IDGQSFAFTADEHIRTEYSHKYTLEQFAGMATAAGLAVRKVWTDRASLFSVQYLTRA"
     gene            complement(6920..9082)
                     /locus_tag="VGB85_22390"
     CDS             complement(6920..9082)
                     /locus_tag="VGB85_22390"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0136855.1"
                     /translation="MSVEAQIAELRARLSLLQGTPTLLLAVAQSDAVVDETRRILLEM
                     LRATPLELADLGPCKIDTGPARWAELTRQHADAVAYALTFIPTTTLEAKVFAQRLNAE
                     RQWLRELAGPVVFVVSRTTERALRQHAQDFVTWVAHSDELPAPQELAALAPRLGVSQA
                     AVTPKLPPEPPVRFLHISDLHLDASRRYDQDRVLDGLLTFLERDRTGFALDLVFVTGD
                     LASTGKPAQYELVVALFKELMLRTGVPAERFFVVPGNHDADREVGRWLLRTLSSDAEA
                     TAFFEDARSRQFHAQKLAAYQQSMQALLGATRPLGIGVGEDSVEIVEIRGARLAVAAF
                     NSAWFAQGDDDREKLWVGEANVMRAVDRIADEEATFAVAMLHHPFKDLHPLDRELVEP
                     WMERGFDLVLRGHLHADKTRSIVTQRGGFVELAAPAAYQGSSWGNGCFLGEIRPRERT
                     VRVRPFMYTAGPDPWVLDPRVFPDDAEEGYCRTFAVPAKRRIKSAVGRPLRAATAAAM
                     RSLPIERQKAIERQAGQNSDQLASELSQEDLMRGVTYLGNMGGWDSGIGWVAAITRAG
                     EDLDFAGRITVKDVDTLQDALTAAGRLFWAAAARRKVKKDLYERTCRAAFTAAFAAVV
                     NGRVMPEQEFGDRPRSGRADILLETMGRRVVINIARETSETILLTEGLDRLSRSIDLA
                     GAELGALVLLGPLAITSSGPKITRATSATGMPILIVVL"
     gene            complement(9079..10347)
                     /locus_tag="VGB85_22395"
     CDS             complement(9079..10347)
                     /locus_tag="VGB85_22395"
                     /inference="COORDINATES: protein motif:HMM:NF019313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0136856.1"
                     /translation="MVDVRAKFRELTQVFDPIYQPSQAAEGGPGKSYVSETHEDYRRQ
                     LDASLDLNDNAKLLVAGQPGCGKTTLLLSLAEDLRRQGRLVAFVDLEAHTAVQDLDPV
                     EMYLAALAEILAETERRGVSVEREALDSCHTWLTRLRLDAEPSTVPELSTSLRGLLSI
                     ARSDQAMRDEMRLRMKPGTEDDPLLLLSCVLRSLASLRPVVILDGLDKLPPNRASQTF
                     LDDKKKPMAEAPGAAILTIPLSVVYEPTFNVLSGRYNNADSAVLPAVRLWDFEPRTRE
                     RRPSDKGMNILRKIIAARVDPIDTRIVLPAVVDRAIVGSGGNIRELARLMQASVVKAY
                     ARRSSFIELQDIEAAIADQRESFRRAYDPSFRTLLLRVRTDYRLDDSSDVAKQLLYGL
                     WVVEYRNGSAWYSLPEPVEQLLQHMERDRA"
BASE COUNT         1560 a         4110 c         3254 g         1605 t
ORIGIN      
        1 tgggccgccc gcgccccgct cctgctgatt ctgatcgccc gccgcaactt cacccacaac
       61 aacaagccca accgcaacgc cgccttcgac gccggcgccg cctggatgtc gctcgccttg
      121 caagccgagc gcctcggcct ccacacccac gcgatggccg gcttcgacac cgcgcgcgcc
      181 tacagcgagc tcggcgtcga ccccgaggcc tacgaggccc tcgctgcgat cgccgtcggc
      241 cggcgcgacg agcccgagac cctccccgag ccgttgcgcg cccgcgaggt cccgagcggt
      301 agaaaccccc aggccagctt cgtattccac ggccgcttcc ccgcgccgac gccatgaccg
      361 cggcgcggcc gccacggccg tgcacacccc cggcccttga ccgcgggcga accgtgttca
      421 cgctgaaccc cgcaatgtcc tggtcctgga acatcggccg tctgttcggc atcgacacgc
      481 gcgtccacgc ctccttcgtg ctgctgctcc tgtgggccgc cttcagcagc tacaccagcg
      541 ccggcaccgt gaccgccgcg gtgatgggcg tggtcttcat gatcgcggtg ttcgcctcgg
      601 tggtcgcgca cgagctcggc cacgcgctga ccgcccgcgt gcacggcatc gagacccgcc
      661 agatcctcct gctcccgatc ggcggcgtcg cccagctcga gcatggccac atgcgccccg
      721 gcgtcgagct gcaggtcgcc ctcgccggcc ccgtcgtcag cttcctgctc gccggcgtgc
      781 tgttcacctt cgccgccgtc gcgggcgacg tcagccccga ctcgttcatc ggcagcctcg
      841 cgtggaccaa cctcggcctc gccgtcttca acatgctgcc cgccttcccg atggacggcg
      901 gccgcgtgct gcgggccgcg ctgtcgagcc gcatgggtca cctgcgcgcc accgacatcg
      961 ccgtcaccct cggcaaggcc ggcgccgtgc tgttcctggt cctcggcctg ttcaccggcc
     1021 gcttcatgct gatgctgctc gccgccttcc tgtacttcgc cgcgcgcagc gagccaaggg
     1081 tcttgccgca ccccggcccg cgccgcagcc agggcatcca caatatcatg ggcgcccccg
     1141 ggtacgatcc cgccctcctg cgcgacggcg acgaccccga cctcgtgctc gcccgcagcc
     1201 cccgcggccg cctctgggtc ctgcgccgca acgacccgcg ctggaactga cagcccccgc
     1261 cctgacgccc gccgaccccg gcgggcgtcc gcgtttaatc tgtccccggg gacatgtcgc
     1321 gtacggcccc cacgacgaac ccggctcccc gcgaatatcc tcgcagcatg tcgccgcatc
     1381 gcctgctcgc gctcctgctc ccgctcgccg cctgcccctc cggcgccgac gaaaccagcc
     1441 tgaccgccag caccggccct ggcaccaccg gcgccccgga cacgggcagc tcgccgacca
     1501 ccggcgacac cacggacgtc ccgaccaccg cgaccgcgac gagcgagccg accaccgcga
     1561 ccgcgacgac cgacccgagc acgaccgacg cgccgaccac ggacaccacc gacgacacca
     1621 ccaccgacgc tccgccggcg atgccgaccg tcttgctgcc gcgcgcatcg atcacgaaca
     1681 ccgagctggc ggtcctcgtc aacgaccagg acccgcagtc gatggccgtc gccgcctact
     1741 atcaacaggc gcgccagatc cccgcgcaga acgtgatcac cgtcagcttc ccgccgaccg
     1801 agaccctcgc caccgacgcc ttcaattcac tcaaggccca gatcgacgcc gccgccggcc
     1861 cgcagatcca ggcgttcgcg gtcgcgtgga ccaacccgca caaggtcgac tgccagtcgc
     1921 tcgtcagcgc cctcgcgctc ggggccgaca tgaaatattg ccagccgcag cccccgtgca
     1981 acccgacggc cccgggcggc ttctatgact cggcctccct cgctcccttc acggacctcg
     2041 gcatccggcc cgccatgcac ctcgcgggcg tcactgccga cgacgttttt aaattgatcg
     2101 accgcggcgt cgcggcggac aacacctacc cctcggcgac cggttatctc ctgcgcacca
     2161 ccgacgtcgc ccgctccgtg cgctttttcc agttcgaggc cgccaaggcc gcctgggctt
     2221 acgacggcgg cctcgcgctc gagtacatcg acaacagcgg cggcatgggc ctcgactaca
     2281 tcgagggcaa gaccgacgtc ctgttctatc tgaccggcct caccgaggtc ccgcagatcg
     2341 ccagcaacat gtacctgccc ggggcgatcg ccgaccacct gacgtcgatc ggcgggcaca
     2401 tcccggagaa cgccggccag atgagcatcg tgcgctggct cgaggccggc gcgaccgcca
     2461 gctacggcac cgccttcgag ccgtgcaatt acaccaacaa gttccccgac gccggcgtgc
     2521 tgctcccgca ctactttcgc ggcgagaccc tgatcgaggc ctactggaag tcggtcgact
     2581 ggccgggcga gggcgtgttc gtcggcgagc cgctcgcgcg cccgtggggc gggccgatct
     2641 tcacctggga gccgccgatg ctgaccgtcg agacttacca gctcgtcccc ggcaagctct
     2701 acaccctcga gtccggccct ggcgtcgacg gcccctggac cctcgtccag gacgacatca
     2761 tgaccaccca gaacaagcgc accgcgatca cgatcgccga cgccagcgag cccttctatc
     2821 gcctcaccgt gaaaccctga gcccgggcag ctggtagcat gcgccatggc ctggcctcgc
     2881 gcgatcacgc tcgctctcct ctcgctcgtc ggatgtacca gcgagcccga cgctcccacc
     2941 acccaggaca ccagctcgag caccggcgtc accactggca gcaccggcgc gaccgggacc
     3001 accaccggca ccaccggcga accgacgtcc gggaccaccg ccgccgacgg ctgtgccagt
     3061 catccgcccg gcgactgggc cgcgtgtcaa aagggcggcc tgaccgacaa cagcctgtgc
     3121 ggctggatgg agggctcggg caacggcatg atcacctgcc tgagcccgca gagcggcagc
     3181 ttcaacgtgt gtgggatccg cgactgcgtc gatgactgcg actgcttcgc cgcgcccctc
     3241 accggcgacg ccgtgccgtc gtgcagggtc gtgttcggcg gcggcggcac ggcctgcgtg
     3301 ctctactgcg ccaacggcca gacctgcccc gatggcatgg agtgctccag cggctactgc
     3361 tactggccca actgagctcc catgcgcctc gcctacctct cgcactactt cccgccggag
     3421 accaacgccc ccgccgcgcg cgtgcacgag ctctcgcgcg ccctcgtgcg cctcggccat
     3481 gaggtccacg tcgtcaccgg ccagcccaac cacccgcgcg gcgtcgtgtt ccccggctac
     3541 cgcgcggccg agtatcgccg cgaggtcgtc gacggcatcg tcgtgcaccg cgtgccgacc
     3601 ttcccggccg ccaaccgcgg cgtgttgcta cgcggcctcg gctacgccgc cgtgcccacc
     3661 gcccaggtcg cgctcggctt gttaaaaatt ccccgcgtcg acctcgtgct cgcgacctcg
     3721 ccgcagatct tcaccggcgt cgccggcctc gcgctcgcgc ggctgcgccg ggtgcccttc
     3781 gtgctcgagg tgcgggacct gtggcccgag tcgatcgtca gcgtcggcgc gctgccggcc
     3841 aggcaccccg tcatccgcgc gctcgagcgg gtcgagcacc atctgtaccg cagcgccgcc
     3901 ggcatcgtct cggtcaccga tagcttcgtc gagcacttcg tcgcccacgg cgtcgcgcgc
     3961 tcgcggatcg ccgtcgtcaa gaacggcgcc gacctcgacc tcttcgaccc ggacaccccc
     4021 ccgaccccgc tgcgcgcccg cctgggcatc cccacagacg cgctgctggt gatgttctgc
     4081 ggcacgatcg gcatggccca cgacgtcggc ctgctcgccc gcgccgccgc gatcgtgccc
     4141 gacgacctcc ccgtgcactt cgtgatcgtc ggcgacggtg ccggccgcga ggcgctcgcc
     4201 gccgagatcg ccgcgctcgg cgtcggcgac cgcgtgcact tgctgcccgc cgtgccacgc
     4261 agcgccgtcc ccggcctgct ggcggccgcc gacctcagcg cgatcgtgct gcgcgacagc
     4321 ccgaccttcg ccggggtctt gccctcgaag atcttcgagg cgatggcgat gcgcgtgccg
     4381 atcttgctcg gtgttcgtgg cgaggcgcgg gcgctggtcg agcgcgccgg cgcaggtctc
     4441 ccgttcgagc ccggggatcc gcgcgacctg gttgcaactt tgcgcgcctt tgccatgctt
     4501 ggggcccccg gccgccgggc catgggggac gccgcgcgtg cccacgcgct gaccgatcat
     4561 gaccgcaccc gccaagccca gcagctcgcc gtcttcctcg agcgactcgg cggagccggc
     4621 cgatcacagt gagctcgacg cgatccgggc cgcctcgctc gcgctcgtcg cctcgctgtc
     4681 ggccgaggac atgctcgccc agtcgatgcc cgacgccagc ccggtcaagt ggcacctcgc
     4741 ccacacgaca tggttcttcg agaccttcgt gctcgccgag ctgcccgagc atcgcccgtt
     4801 ttccccgagc tacgcgagca tcttcaacag ctattataac agcgtcggcg ccccacaccc
     4861 tcgcgcccag cgcggcctgc tgacccgccc cgacctcgcc gaggtcctgc gctaccgcca
     4921 gcacgtcgac cacgccctcc gccgcgcgct cacgcagggc ctgctgtccc ccgagctgcg
     4981 cgcgcgcgtg caggtcggcc tgcaacacga gcagcagcac caggagctga tcctcaccga
     5041 cgtccagcac ctgctgttcc agaacccgct gcgcccggcg tttcgccggc ccaccgacga
     5101 catgtccttc gggacagccg cattatctac atcatccgca tcatctacat catctacatc
     5161 atccgcatca tccgcatcat ccgcatcatc cgcggcgccc gacgcgtggc acagctatcc
     5221 cggcggcgtc gtccaggtcg gtcaccgcgg cgacgacttc tgcttcgaca acgagctgcc
     5281 gcgccaccgc atcctgctcg cgcccttcga ggtcgccacg cgcccggtca gcaacgccgc
     5341 gtacctgcaa ttcatccgcg acggcggcta ccggcgcccg gagctgtggc tgtccgacgg
     5401 ctgggacgcc gtgcatcgcc ggggctggtt cgcgccgctg tactggtccg acgacctcgc
     5461 gacccgcttc acgctgcacg gcccgcgccc gatcgacccg tgggcgccgg tgtgtcacgt
     5521 ctcgtactac gaggccgacg cctacgcgcg ctgggccggc gcccgcctgc cgaccgagca
     5581 cgagtgggag agcgccgccg agggcctgcc cgtcaccggc aacttcgtcg aggccggccg
     5641 cctcgagccg gtgcatgctc acacgccgca cttcttcggc gacgtgtggg tttggaccgc
     5701 gagcccttac gtcgcatatc cgggcttcaa gccggccgcc ggcgctctcg gcgagtataa
     5761 cggcaagttc atgtgcaacc agatcgtgct gcggggtgga tcgtgtgcca cgccgcgcac
     5821 ccacatacgg gccagctacc gcaacttctt cccgccggac gcccgctggc agttctccgg
     5881 tctccgcctt gcgaggtgat catgctcgaa gtccagcgcc gaggccctcg cgtcccgtcc
     5941 atccctgtcg gcaaggcgct cccgctgctg gacctcgcgc ccgaggccgg tgacctgcgc
     6001 cgcgacgcgc tcgccgggct cgcggcttcg ccgaagaccc tgccctgcaa gtatttctac
     6061 gacgaccacg gctcctggct cttcgaccag atctgcagcc tgccggagta ctacccgacg
     6121 cgcaccgagc tggcgatctc ccaccagtac gccgcggaga tggcggccgc ctgcggcccg
     6181 caggtgctgc tgctcgagct cggcagcggc agctcgacca agacccgcgt cctgctcgac
     6241 caccttcgcg cccccgccgg ctacgtgccc gtcgacatcg cccgcgagtt cctgcgcagc
     6301 gccgccgacg acctggcctc ggcctacgca ggcctgcccg tgctcccggt ctgcgccgac
     6361 ttcacgcggc ccttcaccgc cccggtgttg ccggcgcgcc gcaccgtcgt ctacttcccg
     6421 ggctcgacga tcggcaactt caccgccggc gaggcgacgc tgttgctgcg caacatcggc
     6481 cgactcatgg gcgacgccgg cggcctgctg atcggcgtcg acctgcgcaa agacaccgcc
     6541 gtcctcgagc gcgcctacaa cgacgcccgg ggcgtcaccg ctgcttttaa tttaaacctg
     6601 ctgacacgcc tcaaccgcga gctcgcggcc gacttcgacc tcacccagtt ccagcaccgc
     6661 gcgatctggg acccgggccc cggccgcatc gagatgcagc tcgtcagcac ccgcgcccag
     6721 accgtgcaca tcgacggcca gagcttcgcc ttcaccgccg acgaacacat ccgcaccgag
     6781 tactctcata agtacacact cgagcaattc gccggcatgg cgaccgccgc cggcctcgcg
     6841 gtccgcaagg tctggaccga ccgcgcgagc ctgttcagcg tgcaatacct gacccgcgcc
     6901 tgaatccgcc tgcggccggc tacaacacca cgatcaatat tggcatgcca gtagcactcg
     6961 tcgctctggt gattttgggc ccgctggaag ttatcgcaag cggcccgagc aagaccaagg
     7021 ctccgagctc ggccccggcg aggtcgatgg aacggctgag ccggtcgagg ccctccgtga
     7081 gcaggatagt ttccgatgtc tcgcgcgcta tattgatgac cacccgtcga cccatcgtct
     7141 cgaggagtat gtccgcgcgc cccgagcgcg gcctatctcc aaattcctgc tcgggcatga
     7201 ctcgaccatt cacgacggct gcgaaggctg cggtaaaagc cgcacgacac gtccgttcgt
     7261 agagatcttt ctttactttt cgccgggccg ccgccgccca gaacagccga ccggccgcag
     7321 tgagcgcatc ctgcaaagta tcgacatcct tgaccgtgat tcggccagcg aaatcgaggt
     7381 cctccccggc tcgggtaatg gcggccaccc agccgatgcc ggaatcccac ccccccatat
     7441 tcccgagata tgtgacccct ctcatcaggt cttcttgcga tagctcggat gcaagttggt
     7501 cactattctg gcccgcctgc cgctcgatgg ctttctgccg ctcaatcggc aacgagcgca
     7561 tcgccgcggc cgtcgccgct cgcagcggcc gacccaccgc gctcttgatc cgcctcttcg
     7621 ccggcaccgc gaacgtacgg caatagcctt cctccgcgtc atccgggaac actcgcggat
     7681 cgagcaccca gggatccggc ccggcggtgt acatgaaggg ccgcacgcgc accgttcgct
     7741 cgcgtggccg gatctccccc aaaaaacacc cgtttcccca gctgctcccc tgataggccg
     7801 cgggggccgc gagctcgacg aagccgccgc gctgggtgac gatcgaccgc gtcttgtcgg
     7861 cgtgcaggtg ccccctcaac accagatcga agccgcgctc catccacggt tcgaccagct
     7921 cgcggtccag cgggtgcaga tctttgaagg ggtgatgcag catggcgacg gcgaaggtcg
     7981 cctcctcgtc ggcgatgcga tcgaccgcgc gcatcacgtt agcctcgccg acccacagct
     8041 tctcacggtc gtcgtccccc tgggcgaacc acgccgaatt gaaggcggcg acggcgagcc
     8101 gggccccgcg aatctccacg atctcgacgc tgtcctcgcc gacgccgatg ccgagcgggc
     8161 gcgtcgcgcc gagcagcgcc tgcatgctct gctgataagc agcgagcttc tgcgcgtgga
     8221 attgccggct ccgggcatct tcaaaaaatg cggtcgcctc cgcgtcgctc gacagcgtcc
     8281 gcagcagcca ccgaccgacc tcgcggtcgg cgtcatggtt gcccgggacg acgaagaagc
     8341 gctccgccgg cacgcccgtc cgcagcatca attccttgaa gagcgcgacg accagctcgt
     8401 actgcgccgg cttgccggtg ctggccagat ctccggtcac gaacacgagg tccagcgcaa
     8461 agccggtacg atcgcgctca aggaaggtca gcagcccgtc gaggacacga tcctggtcgt
     8521 atctccgcga tgcatcgagg tgcagatcgg agatatgcag gaagcggacc ggcggctcgg
     8581 gcggcagctt cggggtcacg gccgcttgcg acacgccgag ccggggcgcc agcgcggcga
     8641 gctcctgcgg cgcaggcagc tcgtcgctgt gcgccaccca ggtgacgaag tcctgcgcgt
     8701 gctggcgcag cgcccgttcg gtcgtccgcg acacgacgaa gacgacaggc ccggcgagct
     8761 cgcgcagcca ctgccgctcg gcgttgaggc gctgcgcgaa caccttggcc tcgagcgtgg
     8821 tcgtcgggat gaacgtcagc gcatatgcga cagcgtccgc gtgctgtcgg gtgagctcgg
     8881 cccagcgcgc gggtcccgtg tcgatcttgc acgggcccag gtcggcgagc tccagcggcg
     8941 tggcgcgaag catctcgagc aagatgcgcc gcgtctcgtc gacgaccgcg tcgctctggg
     9001 cgacggcgag cagcagggtc ggcgtcccct gcagcagcga gagccgcgct cgcagctcgg
     9061 cgatctgcgc ctcgacgctc acgcgcggtc gcgctccatg tgctgcagca gctgctcgac
     9121 cggctcgggc aggctgtacc aggcgctgcc gttgcggtac tcgacgaccc acaatccata
     9181 cagcagctgc ttggcgacgt cgctcgaatc gtcgagtcga tagtcggtgc gcacgcgcag
     9241 gagcagcgtg cgaaagctcg ggtcgtaggc cctgcgaaac gactcgcgct gatcggcgat
     9301 cgccgcctcg atgtcctgga gctcgatgaa gctgctgcga cgcgcgtacg ccttgaccac
     9361 gctcgcctgc atgagccgcg cgagctcgcg gatgtttcca cccgacccga cgatcgcgcg
     9421 atcgacgacc gcgggcagga cgatcctggt gtcgatgggg tcgacccggg ccgcgatgat
     9481 cttgcgcagg atgttcatcc ccttgtccga gggcctgcgt tcgcgggtcc gcggctcgaa
     9541 gtcccacagg cgcacggccg ggagcaccgc gctgtcggca ttgttatatc tgccgctgag
     9601 cacgttgaac gtcggctcgt acaccacgga caatgggatg gtcaggatcg ccgcgccggg
     9661 cgcctcggcc atcggcttct tcttgtcgtc gaggaaagtc tggctggcgc gattgggcgg
     9721 gagcttgtcc aggccgtcga ggatgaccac cggccgcaag ctcgccaggc tgcgcagcac
     9781 acaggacagc agcaggagcg ggtcatcctc ggtgcccggc ttcatgcgca gccgcatctc
     9841 gtcgcgcatc gcctgatcac tgcgcgcgat cgacagcagg ccgcgcagcg aggtcgacag
     9901 ctcgggcacg gtcgagggtt cggcgtcgag ccgcagacgg gtcagccatg tatgacagct
     9961 gtccagcgcc tcgcgctcga cggacacccc gcgtcgctcg gtctccgcga ggatctccgc
    10021 gagcgcggcg agatacatct cgaccggatc gaggtcctgc accgccgtgt gcgcctcgag
    10081 gtcgacgaag gcgacgagcc gcccctggcg acgcaggtcc tccgcgaggc tgaggagcag
    10141 cgtcgtcttc ccgcaccccg gctgaccggc gaccagcagc ttcgcgttgt cgttgaggtc
    10201 gagcgacgcg tcgagctggc gacgataatc ctcgtgcgtc tccgacacat acgacttgcc
    10261 gggcccgccc tccgcggcct ggctcggctg gtagatcggg tcgaagacct gcgtcagctc
    10321 cctgaatttc gcgcgtacgt cgaccatgac gccccggggc gatcgcttgc aggccaacgt
    10381 acccgcgcca gcgaggcgcg gcaagcaaac cacagcgatc ctgcgcgaca ggcgctcggc
    10441 gcggtcgcgg ggtcaggccg cccgcgtcgc cggcagcagc tcgaccaggc ggtcgagcgc
    10501 cgccgcggcc ccacgcctgc gcagggccg
//
LOCUS       DASWNJ010001343        10660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_201095, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001343 DASWNJ010000000
VERSION     DASWNJ010001343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 10660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10660
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201095"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VGB85_22400"
     CDS             complement(<1..193)
                     /locus_tag="VGB85_22400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136857.1"
                     /translation="MPMSIFTPRTAATAGSYLGLLALLCVGRVAHAQAPMGGGMGGMG
                     GMGGGGMPQGPGQGGKPDKK"
     gene            305..1450
                     /gene="nagZ"
                     /locus_tag="VGB85_22405"
     CDS             305..1450
                     /gene="nagZ"
                     /locus_tag="VGB85_22405"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEY0136858.1"
                     /translation="MTRTTSLWQPGQLLFAGFQGKELPRALAELLAQGRQGGVILFAR
                     NIGDPAQVRALTAALHAAAPPEAPVLLAIDQEGGRVQRLRAPWTEWPPMRLLGERDDP
                     ATTAAVASAIARELADLGIGLDFAPVVDVDTNPDNPVIGDRSFSRDPARVGRHAAAFI
                     TAMQDAGVAACAKHFPGHGDTTTDSHLELPRLEHDLHRLREVELPPFKAAVAAGVASI
                     MTAHVLFPRLDKKRPATLAPDVMDLLRGELGYDGVVFSDDLEMKAVADHFQPEPLVRG
                     CLEAGCDAVLVCSQFDLVREALRLLEKTPDALLEHPLRRMLAFKQRYAPAHRRRALGL
                     PDPAAKPPALLADLFAAPELLRTPEPVVETPPFAEHQALAEQIRRER"
     gene            1935..2672
                     /locus_tag="VGB85_22410"
     CDS             1935..2672
                     /locus_tag="VGB85_22410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136859.1"
                     /translation="MLASAALAAASVPEANATEPAHLTWAKYLVETITPANNYYGDPG
                     SVLWKGENGLTTSKNRTRCAPLLTQLLKKAYSTDMVGWLGCTSPHAATYHDAIEVEDG
                     FELIESISDIKPGDIIAIEYLDEDCDNLTCGDFSGCNTTGHVAIVLEAPTPRYETSPL
                     IKGTEQYSVRIIDSSATPHGINDTRYQADKYEWDDQGVGRGRMRLYVDADDPDQPIVG
                     YTWSTSVGSTYYPYYKRDLVIGRFKKT"
     gene            complement(2674..5034)
                     /locus_tag="VGB85_22415"
     CDS             complement(2674..5034)
                     /locus_tag="VGB85_22415"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEY0136860.1"
                     /translation="MRVSPPLAALAFLLAGCTDSPAIEEPGTSTGTTSTSTTDTPTTG
                     ATSDDTTASEPPGPTGMPASPPLTQWVDPFIGTGGKGFGTGSAFPGPQRPFGLARPGP
                     DTALAGGLALSFAHCSGYSFDDTMVMGLSQTRMHGTGIVDYGAISVMPTLGFTPEKAA
                     PKGVMQALDKASEQASPGRYAVTLADGIGVELTATDHVGMHRFTFPEGQPAAVVLDLG
                     RTLPDVEVVEAELTIDDAGAHGFMHVSGGYSGRFGGVKIYFAAKLSRPLTGHGTWDDG
                     VITADGAGAKGQAIGAYFEFSNTEPVVLGVGLSFVDAEHAAQNLAAEAPALDFDAART
                     EAEAAWEALLGRVELHGTSEREFEIFYSALYHTLLMPTLASDTDGSYRGLDGEVHTAD
                     FPYYTDLSLWDTFRTEHPLLTLLYPEFQKNFLRSLARMAKDGGYMPRWPLGTGYTGGM
                     LGESATIVFADSYAKGVTIPADEAAVAYAAMRKTAMAPVPADSPYPGRSGVLDYIELG
                     YVPQEAGGSSVSWTLESAYDDFALASLATALGEPDDAAMFAARAGNYRNTYDPTSGLM
                     VARRKDGSFVADIDPLGWQDFYAEGNAMQYLWYVPHDIVGLAELMGGRAVMLDRLRDL
                     FEKSEAEVVGIMPQQYYWHGNEPDLHAAFIFSALGEPAEAARWSRWIGDRRYNDGPGG
                     LAGNDDAGTLSAWYVLAASGLYPIAGTATYLVGSPRFTRMTLHLAGGDLVIDAPEASA
                     DAIYVDAATLDDTSITTPSLRHAELAAGGLLRFDMAQTPGPWGQAP"
     gene            complement(5088..7169)
                     /locus_tag="VGB85_22420"
     CDS             complement(5088..7169)
                     /locus_tag="VGB85_22420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0136861.1"
                     /translation="MSLLAPLMLLGLLGLGLPLLAHLRGKEEPKVVRFAGLRFLAAPD
                     EVITQRRRLRDVLLLLVRLGLLALLVFALARPVSTGDARVAVLGEPHDALLLLDGSFS
                     MALRTGDESLRAASEDAAAAILEALPPGSRVGLLTTDPAGPRLELSADPDRVRAAVTA
                     WAAEPPRAGAWPLESGLAGAVALLGQAGEGPGRRVVYALGDFTGRGLGSLPATAAADI
                     PVLPVAIRGELGAPPPVPEHMSLESALWTPARELDPRAVRISGEVRRHGPADGPSKRV
                     ALALRVDGEIAARAEIDVPADGRAPYEFTHTSATAEPTPASVELLLADDPLPGDEVRH
                     LWLAGEDGVEVALVNGDPSEMRAHDELYFLATALTSGPDAQRLRIHSLAPDQLETRLR
                     EGDRLAGVDVLVLANLRAPGEDLAPLLKSRVAEGMGLWISVGDRVEARAYNERLGDLL
                     PLLLREPLLVGTAPGRTEARTEGVAPPNLAHPILLGLQGELGLLGTRTRRLFLLEPDP
                     SRGAEVALSFTSGAPALLSRPYQRGRVVLLTTSIDRDWSDLPLRPGFVPLAERVLAWL
                     DSSHPGGAAGQILAGEPRIIATDKPVTVRLPDGRERILPPEREGQAVFKDTYTPGHYL
                     VTTAAGEPLTFAVQQDPAESDTTPVPVRPPSLDGDQVMAAAQHPRWRPLVLLAAALLL
                     LETLLRARARR"
     gene            complement(7231..8193)
                     /locus_tag="VGB85_22425"
     CDS             complement(7231..8193)
                     /locus_tag="VGB85_22425"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY0136862.1"
                     /translation="MSADPAQALAAVVPPELRDLLRGRQLVLARPVGGPHAGRHASTR
                     AGLGDQFRGHRPYVPGDDPRRLDWRAVARRDRLVLRQTDSEDALSTVLLVDGSGGMAH
                     GTGATNKQRHAAALAGALAHLALRQGDRVGFAVGRDDALELSQLRPQAGSPRLAALAQ
                     ALLRPPAGLCPWPALLAAVAPALPRRSLVVALSDFLDPDPRSADADAQLHRGLAGLRS
                     RGHDVVLVQVLHRDELEFPWAGRAPWRFEDPRGVREPIEGHAAGLREAYLERLHAHLR
                     ALDLACGEAGLHLHRSVSDRPLAASLLALLARLAGNPQPAEATP"
     gene            complement(8233..9309)
                     /locus_tag="VGB85_22430"
     CDS             complement(8233..9309)
                     /locus_tag="VGB85_22430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY0136863.1"
                     /translation="MPSAQPPPPSPPSPPSHAPPRPPAGSSGSGDAGGDRAALQALAR
                     SRERLVEQLHHRVVGQQGVIDLLLICLFAGGHGLFVGVPGLAKTLLVSSLAQCLDLQF
                     SRVQFTPDLMPSDITGSEVLEEDPATGRRQFRFVAGPVFCNLLLADEINRTPPKTQAA
                     LLQAMQEARVSAGGRTHPLPQPFHVFATQNPIEQEGTYPLPEAQLDRFTLQIVVRYPD
                     EAAERQIVALGSDGGTAISPVLTPAELTTHQALVRRIPVPDSVVAHVVALLRATRPGE
                     SDCPPAVRELLQWGAGPRAGQQLVAAAQARAALDGRPAVTIADVRALAEPVLEHRLVR
                     SFAAESRAVATAEILHRVLAAVPG"
     gene            9438..10607
                     /gene="moeB"
                     /locus_tag="VGB85_22435"
     CDS             9438..10607
                     /gene="moeB"
                     /locus_tag="VGB85_22435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEY0136864.1"
                     /translation="MASFSDILSQVKRSIREVSVDETKAQVLGGTGDKPVLIDIRERD
                     EYEQGFIPRAEWIPRGFLEMKIEDLVPERGREIILYCAGGNRSALAARSLEELGYTKV
                     SSMAGGFRAWKQSGHAFDRPRSLTAEQIKRYSRHIMLPEVGELGQGKLLDAKVLCLGA
                     GGLGSPSALYLAAAGVGTLGMVDDDLVDESNLQRQVLHNMERLGMPKVESARMTLQAL
                     NPDVKVIAHQTRLNSENIMEILAGYDLVVDGADNFPTRYLLNDAALKLGKPVIHASIF
                     RFEGQLTTFLPFDGPCYRCLYPDPPPPGMAPSCQEAGVLGVLPGIIGTLQANEALKVI
                     LGAGTSLSGRLLVFDALGTKFRTLKLRKDPECRVCSKKPEEIELIDYEAFCSLAQ"
BASE COUNT         1546 a         3501 c         4146 g         1467 t
ORIGIN      
        1 ccttcttgtc gggcttgccg ccctgaccgg gtccttgcgg catgccgccg ccacccatgc
       61 cgcccatgcc tcccatgccg ccgcccatcg gcgcctgcgc atgggcgacc cgaccgacgc
      121 agaggagcgc aaggaggccg agataggatc cggcggttgc ggcggtgcga ggcgtgaaaa
      181 tcgacatcgg caaggcgccg gacgctaaca accgcgccgg ggggcgtcaa ccgcaggctt
      241 gccgcggagg cgggccctga tgggagtttg cacgcggctg cgcgagcctc taacctgccg
      301 gccgatgacg aggacgacga gcctgtggca gccgggtcag ctcctgttcg cgggattcca
      361 gggcaaggag ctgccgcgtg cgctggcgga attgctggcc cagggccgtc agggcggggt
      421 gatcctgttc gccaggaata tcggcgaccc ggcgcaggta cgggccctga cggcggccct
      481 gcacgcggcg gcgccgcccg aggcgcccgt gttgctggcg atcgaccagg agggtggtcg
      541 tgtccagcgg ctgcgggcgc cgtggaccga gtggccgccg atgcgtctgc tcggcgagcg
      601 cgacgatcct gcgacgaccg cggcggtcgc gtcggcgatc gcccgtgagc tggccgacct
      661 cgggatcggc ctcgacttcg ccccggtggt cgacgtcgac accaaccccg acaacccggt
      721 gatcggcgac cgcagcttct cgcgcgaccc ggcccgcgtc ggccggcacg cggcggcctt
      781 catcacggcg atgcaggacg cgggggtcgc cgcgtgcgcc aagcacttcc ccggccacgg
      841 cgacacgacg accgacagcc acctcgagct gccgcgcctc gagcacgacc tgcaccgcct
      901 gcgagaggtc gagctgccgc cgttcaaggc ggccgtcgcc gccggcgtgg cgagcatcat
      961 gacggcgcac gtgctgttcc cccgcctcga caagaagcga cccgccacgc tcgcgcccga
     1021 tgtcatggac ctgctgcgcg gcgagctcgg ctacgacggc gtggtgttca gcgacgacct
     1081 cgagatgaag gcggtcgccg accacttcca gcccgagccg ctggtccgcg gctgcctcga
     1141 ggccggctgc gacgcggtgc tggtgtgcag ccagttcgac ctggtgcgcg aggccctgcg
     1201 cctgctcgag aagacccccg acgcgctgct cgagcacccg ctgcggcgca tgctggcgtt
     1261 caagcagcgc tacgcgccgg cgcaccggcg gcgggcgctg ggtctgcccg acccggccgc
     1321 caagcccccg gcgttgctcg cggacttgtt tgccgcgccg gagctgctgc gcacgccgga
     1381 gccggtggtc gagacgccgc ccttcgcgga gcatcaggcg ctggccgagc agatccgtcg
     1441 cgagcgctga gcggtcggcg tcccgggacg cggacgcttc gcgagcttgt atgggcaata
     1501 cgagcttcgt gaggcgttcg cgccgcgcgc ccgaggaatt gggctcgaca acccatatat
     1561 tcctattata aagcgggctg ctgttcctgc gattcgtggc ccgggaatgt ccgcagtctg
     1621 cgatgagaga tatcggtgtg ggtgattcaa tgtatcgagg ttgaggatac atatagacat
     1681 cgttgcattg ttgcccacat cgccgatcgg atcacgagga tcgtgccggc gccggtgtcg
     1741 ctgtgcgacg cgccaccgga cacgtgtgcg gggctggaga gacaggtgta tggaaggtcg
     1801 gacggatgct cgcggacttt tgcgcgggag aaggacgaaa gatgcgtacg acgacgcgaa
     1861 tgggccgaac ggtgggaatg atgggaactg gacgaatgat ggcaggctcg tggtcccgtc
     1921 gaatcttcgg atcattgctg gcgagcgcgg cgctcgccgc cgcctcggtg cccgaggcga
     1981 acgcgacgga gccggcgcac ctgacctggg ccaagtacct ggtcgagacg atcacgccgg
     2041 ccaacaacta ctacggagac ccgggcagcg tgctgtggaa aggcgagaac gggctcacca
     2101 cgagcaagaa ccggacccgc tgcgcgccgc tgctgaccca gctgctgaag aaggcgtaca
     2161 gcacggacat ggtcggctgg ctcggctgta ccagcccgca cgcggcgacc taccacgacg
     2221 cgatcgaggt cgaggacggc ttcgagctga tcgagtcgat ctcggacata aagccgggtg
     2281 acatcatcgc gatcgaatac ctcgacgagg actgcgacaa cctgacctgc ggcgacttct
     2341 cgggctgtaa taccaccgga cacgtggcaa tcgtgctcga ggccccgacg ccgcgctacg
     2401 agaccagccc gctgatcaag ggcaccgagc agtactcggt ccggatcatc gactcgagcg
     2461 cgaccccgca cgggatcaac gacacccgct accaggccga caagtacgag tgggacgacc
     2521 agggcgtcgg ccgcggccgc atgcgcctct acgtcgacgc cgacgacccc gaccaaccga
     2581 tcgtcggcta cacgtggagc acctcggtcg gctcgaccta ttatccttac tacaagcgcg
     2641 acctggtgat cgggcgcttt aaaaagacct gagtcacggg gcctgtcccc agggacctgg
     2701 cgtttgggcc atgtcgaagc gcagcaggcc gccagcggcg agctcggcgt gacgcaggct
     2761 cggcgtggtg atcgatgtgt cgtcgagcgt cgcggcgtcg acgtagatcg cgtcggcgct
     2821 ggcctcgggg gcgtcgatca cgaggtcgcc gcccgccagg tgcagggtca tgcgcgtgaa
     2881 cctcgggctg ccgaccaggt aggtggcggt gcctgcgatc gggtagaggc cgctcgcggc
     2941 gagcacgtac caggcgctca gggtgccggc gtcgtcgttg ccggcgaggc cgcccgggcc
     3001 gtcgttgtag cgcctgtcgc cgatccagcg cgaccacctc gcggcctcgg cgggctcgcc
     3061 gagcgcgctg aagatgaagg cggcgtgcag gtcgggctcg ttgccgtgcc agtagtactg
     3121 ctgcggcatg atgccgacga cctcggcctc ggacttttca aagaggtcgc gcaggcggtc
     3181 gagcatgacg gcgcggccgc ccatcagctc ggcgaggccg acgatgtcgt ggggcacata
     3241 ccacaggtac tgcatcgcgt tgccctcggc gtagaagtcc tgccagccga gcgggtcgat
     3301 gtcggcgacg aagctgccgt ccttgcggcg cgcgaccatc aggcccgaag tgggatcgta
     3361 ggtgttgcgg tagttgcccg cgcgggcggc gaacatcgcg gcgtcgtccg gctcgccgag
     3421 cgcggtggcg aggctggcga gggcgaagtc gtcgtaggcg gactctaggg tccacgacac
     3481 gctcgagccg ccggcctctt gcggcacgta gccgagctcg atgtagtcga gcacgcccga
     3541 gcggccaggg taggggctgt ccgcagggac aggtgccatg gcggtcttgc gcatcgcggc
     3601 gtaggcgacc gcggcctcgt cggccgggat cgtgacgccc ttcgcatagc tgtcggcgaa
     3661 cacgatcgtc gcgctctcgc cgagcatgcc gccggtgtag ccggtgccca gcggccagcg
     3721 cggcatgtaa ccgccgtctt ttgccatgcg cgcgagcgag cgcaggaaat tcttttgaaa
     3781 ttcggggtac agcagggtca gcagcgggtg ctcggtgcga aaggtgtccc acagcgagag
     3841 gtcggtgtag taggggaagt cggccgtgtg gacctcgccg tcgaggccgc ggtagctgcc
     3901 gtcggtgtcg ctggcgaggg tcggcatcag cagcgtgtgg tagagcgcgc tataaaaaat
     3961 ctcgaactcg cgctcgctgg tgccgtgcag ctcgacgcgg ccgagcagcg cctcccaggc
     4021 ggcctcggcc tcggtgcggg cggcgtcgaa gtcgagggcc ggagcttcgg cggcgaggtt
     4081 ctgcgcggcg tgctcggcgt cgacgaagct gaggccgacg ccgaggacga ccggctcggt
     4141 gtttgaaaat tcgaagtagg cgccgatcgc ctggcccttg gcgccggcgc cgtcggccgt
     4201 gatcacgccg tcgtcccagg tgccgtggcc ggtgagcggg cgcgagagct tggcggcgaa
     4261 gtagatcttc acgccgccga agcgaccgct gtagccgccg ctgacgtgca tgaagccgtg
     4321 cgcgccggcg tcgtcgatcg tcagctcggc ctcgacgacc tcgacgtccg ggagggtgcg
     4381 cccgaggtcg agcacgacgg cggccggctg tccttcgggg aaggtgaagc ggtgcatgcc
     4441 gacgtggtcg gtggcggtca gctcgacgcc gatgccatcc gcgagggtca cggcgtagcg
     4501 gccggggctc gcctgctcgc tggccttgtc gagggcctgc atcacgccct tgggcgccgc
     4561 cttctcgggc gtgaagccga gcgtcggcat gaccgagatg gcaccatagt cgacgatgcc
     4621 ggtgccgtgc atgcgcgtct ggctcaggcc catcaccatc gtgtcgtcga agctgtaacc
     4681 ggagcagtgc gcgaagctca gggccaggcc gcccgcgagc gcggtgtcgg ggccgggcct
     4741 ggcgaggccg aaggggcgct gcgggcccgg gaaggcgctg ccggtgccga agcccttgcc
     4801 gccggtgccg atgaaggggt cgacccactg cgtcaagggt ggtgacgcgg gcatgccggt
     4861 cgggccgggc ggctcgctgg cggtcgtgtc atcgctggtc gcgccggtcg tcggcgtgtc
     4921 ggtggtgctc gtcgaggtcg tgccggtgct cgtgcccggt tcttcgatcg ccggtgagtc
     4981 ggtgcagccg gcgagcagaa aggcgagagc cgcgagggga ggcgagacgc gcatgccgca
     5041 tcgtagccgg ccatggtcgc ggcgcgcgat ggcgatcgcc cgatccctca acgccgcgcc
     5101 cgggcgcgga gcagggtctc gagcagcaac agggccgcgg cgagcaagac gaggggccgc
     5161 cagcgcgggt gctgggcggc ggccatgacc tgatctccgt cgaggctcgg cgggcgcacg
     5221 gggaccgggg tcgtgtcgct ctcggccggg tcctgctgga ccgcgaaggt cagcggctcg
     5281 ccggcggcgg tggtgacgag gtagtgcccg ggcgtgtagg tgtctttaaa gacagcctgg
     5341 ccctcgcgct cgggcgggag gatgcgctca cgtccatcgg gcagacgcac ggtgaccggc
     5401 ttgtcggtgg cgatgatgcg cggctcaccg gcgaggatct gcccggccgc gcctccgggg
     5461 tggctgctgt cgagccaggc gagcacgcgt tctgccagag gcacgaagcc gggacgcagc
     5521 ggcaggtcgg accagtcgcg gtcgatcgag gtcgtcagca atacgacgcg gccgcgctgg
     5581 tatggacgcg agagcagggc cggcgcgccg ctggtgaagc tcagcgccac ctcggcgccg
     5641 cgcgaggggt cgggctcgag gagaaataat cggcgcgtgc gggtgccgag caggccgagc
     5701 tcgccctgca gtccgagaag gatcgggtgg gcgaggttcg gcggggcgac gccctcggtg
     5761 cgcgcctcgg tgcggccggg cgcggtgccg acgagcagcg gctcgcgcag cagcagcggc
     5821 agcaggtcgc cgaggcgctc gttgtaggcc cgcgcctcga cgcggtcgcc gacggagatc
     5881 cacagcccca tgccctcggc gaccctactc tttaaaagag gggcgaggtc ctcgccgggg
     5941 gcgcgcaggt tggcgagcac gagcacgtcg acgccggcga ggcggtcgcc ctcgcgcagc
     6001 cgcgtctcga gctgatccgg cgcgaggctg tggatccgca ggcgctgcgc gtcggggccg
     6061 gacgtcagcg cggtggcgag gaagtagagc tcgtcgtggg cccgcatctc gctcgggtcg
     6121 ccgttgacga gcgcgacctc gacgccgtcc tcacccgcga gccagaggtg gcggacctcg
     6181 tcgccgggca gggggtcatc cgcgagcagc agctcgacgg aggcgggggt cggctcggcg
     6241 gtggcgctgg tgtgggtgaa ctcgtagggg gcgcggccgt cggcgggcac gtcgatctcg
     6301 gcgcgcgctg cgatctcgcc gtcgacgcgc agggccaggg cgacgcgctt gctcggaccg
     6361 tcggcggggc cgtgacgccg gacctcgccg gagatcctca cggcgcgggg gtcgagctcg
     6421 cgggcggggg tccacagggc tgactcgagg gacatgtgct cggggacagg cggcggggcg
     6481 ccgagctcgc cgcggatcgc cacgggcagc acggggatgt cggcggcggc ggtggcgggg
     6541 aggctgccga ggcctctgcc agtaaagtcg ccgagcgcgt agacgacgcg gcgacccggg
     6601 ccctcgcccg cctgaccgag cagggcgacg gccccggcga ggccgctctc gagcggccac
     6661 gcgccggccc gcgggggctc ggcggcccac gcggtgacgg ccgcgcgcac gcggtcgggg
     6721 tcggcgctga gctcgaggcg cgggcccgct gggtcggtgg tcagcaggcc gacgcggctg
     6781 cccggcggca gggcctcgag gatcgcggcc gccgcatcct cgctggcggc gcgcaggctc
     6841 tcgtcgccgg tgcgcagcgc catgctgaac gagccgtcga gcaggagcag ggcgtcgtgc
     6901 ggctcgccga gcacggcgac ccgcgcatcg ccggtgctga ccgggcgcgc cagcgcgaac
     6961 accagcaggg cgagcaggcc gaggcggacg agtaacaaga gcacgtcgcg caggcggcgg
     7021 cgctgggtga tcacctcgtc gggcgcggcg aggaagcgca ggccggcgaa gcggacgacc
     7081 ttcggctcct ccttgccgcg caggtgggcg agcagcggca agccgaggcc gagcaggccg
     7141 agcagcatca gcggcgcgag caggctcatg cgcgcggctc cacggctgtc cgaagggaca
     7201 ggtgaacggg ggctgtccga agggacaggt tcatggcgtc gcctccgcgg gctgggggtt
     7261 gccggcgagg cgggcgagca gggccagcag ggaggcggcc agggggcggt cgctgacgct
     7321 gcggtgcagg tgcaggccgg cctcgccgca ggcgaggtcg agcgcgcgca ggtgcgcgtg
     7381 caggcgctcg aggtaggcct cgcgcaggcc ggcggcgtgg ccttcgatcg gctcgcgaac
     7441 gccgcggggg tcctcgaagc gccacggggc ccggccggcc cacgggaact cgagctcgtc
     7501 gcggtgcagc acctggacga gcacgacgtc gtggccgcgg ctgcgcaggc cggcgaggcc
     7561 gcggtggagc tgggcgtcgg cgtcggcgct gcgcgggtcg gggtcgagga agtcggacag
     7621 cgcgacgacg aggctgcgcc gcggcaaggc cggcgcgacc gcggcgagca gcgccggcca
     7681 cgggcacagg ccggcgggcg ggcgcagcag cgcctgcgcg agggcggcga ggcgcgggct
     7741 gccggcttgc gggcgcagct ggctcagctc gagcgcgtcg tcgcggccga ccgcgaagcc
     7801 gacgcggtcg ccctggcgca gcgcgaggtg ggcgagcgcg ccggcgaggg cggcggcgtg
     7861 gcgttgtttg ttggtggcac ctgtcccatg ggccatgcca ccggagccgt cgacgagcag
     7921 cacggtcgac agcgcgtcct cgctgtcggt ctggcgcagc acgaggcggt cgcggcgagc
     7981 gacggcgcgc cagtcgaggc ggcgcgggtc gtcgccgggc acgtaggggc gatgcccgcg
     8041 gaactgatct ccgaggccgg cgcgggtcga ggcgtggcgg cccgcgtgcg gtccgccgac
     8101 cggccgcgcg agcacgagct ggcgtccgcg gagcaggtca cgcagctcgg gcgggacgac
     8161 ggcggcgagc gcctgggcgg gatcggcgga cacgcttcga gctgtacctt gggacaggaa
     8221 ggaaaggaca ggtcagcccg gcacggcggc gagcacccgg tggaggatct ccgcggtggc
     8281 gacggcgcgg ctctcggcgg cgaagctgcg gacgaggcga tgctcgagca ccggctcggc
     8341 gagggcgcgg acgtcggcga tcgtgaccgc ggggcggccg tcgagcgcgg cgcgggcctg
     8401 ggccgcggcg accagctgct ggcctgcgcg cggtcctgcg ccccactgca gcagctcgcg
     8461 cacggcgggc gggcagtcgc tctcgcccgg acgggtggcc cgcagcagcg cgacgacgtg
     8521 ggcgacgacc gagtccggca cggggatcct gcgcacgagg gcctggtgcg tggtcagctc
     8581 ggcgggggtc agcacggggg agatcgcggt gccgccgtcg ctgccgagcg cgacgatctg
     8641 ccgctcggcg gcctcgtcgg ggtagcgcac gacgatctgc agggtgaagc ggtcgagctg
     8701 ggcctcgggc agcggatagg tgccctcctg ctcgatcggg ttctgggtcg cgaacacgtg
     8761 gaagggctgc ggcagcgggt gcgtgcgccc gccggcgctg acgcgcgcct cctgcatcgc
     8821 ctgcaacagc gcggcctggg tcttgggggg ggtgcggttg atctcgtcgg cgagcaggag
     8881 gttgcagaac accgggccgg ccacgaagcg aaactgacga cgaccggtcg cggggtcttc
     8941 ttccagcacc tccgagccgg tgatgtcgga gggcatcagg tccggggtga actgcacgcg
     9001 cgagaactga aggtcgaggc actgcgcgag gctgctgacg agcagggtct tggcgagccc
     9061 gggcacgccg acgaacaggc cgtggccgcc cgcgaacagg cagatcagga gcaggtcgat
     9121 caccccctgc tgaccgacga cgcggtggtg gagctgctcg acgaggcgct cgcggctgcg
     9181 ggccagcgcc tgcagggcgg cgcggtcgcc tccggcgtcc ccggacccgg aggagccagc
     9241 aggcggccgt ggaggagcat gggacggcgg tgacggcggt gacggtggtg gcggctgggc
     9301 gctcggcacg gaggcgcgga gtatagcagc cggatccgtg aggaggaccg cgggacggcg
     9361 tttgtcaggc gcctgggatc ttctggtaaa acctggctcg ctggcgcgcg atgcggcagc
     9421 gagggaggca cgcggtcatg gccagcttta gcgacatcct cagccaggtg aaacgatcga
     9481 tccgcgaggt gtcggtcgac gagacgaagg cccaggtcct cggcgggacc ggcgacaagc
     9541 cggtgctgat cgacatccgt gagcgcgacg agtacgagca gggctttatc ccccgcgccg
     9601 agtggatccc ccgcggcttc ctcgagatga agatcgaaga tctggtgccc gagcgcggcc
     9661 gcgagatcat cctctactgc gccggcggta accgctcggc cctcgccgcc cgctcgctcg
     9721 aggagctcgg ctacaccaag gtctcctcga tggccggcgg gttccgggcc tggaagcagt
     9781 ccggccacgc cttcgaccgc ccgcgctcgc tgaccgcgga gcagatcaag cgctacagcc
     9841 gacacatcat gctcccggag gtcggtgagc tcggccaggg caaattgttg gacgccaagg
     9901 tcctgtgcct cggcgccggt ggcctcggct cgccctccgc gctgtacctc gcggcggccg
     9961 gcgtcggcac gctcggcatg gtcgacgacg acctcgtcga cgagagcaac ctgcagcgcc
    10021 aggtcctcca caacatggag cgcctcggca tgccgaaggt cgagagcgcc cgcatgacgc
    10081 tccaggccct caaccccgac gtcaaggtca tcgcccacca gacccgcctc aactccgaga
    10141 acatcatgga gatcctcgcg ggctacgacc tcgtggtcga cggcgccgac aactttccca
    10201 cgcgttattt gctgaacgac gcggcgctca agctcggcaa gccggtgatc cacgcgtcga
    10261 tcttccgctt cgagggccag ctgacgacct tcctgccgtt cgacgggccc tgctaccgct
    10321 gcctgtaccc ggatccgcct ccgccaggca tggctccgtc gtgccaggag gccggcgtgc
    10381 tcggcgtgct gccgggaatt attgggacgc tgcaggccaa cgaggcgctc aaggtgatcc
    10441 tcggggccgg gacgtcgctc tcgggccggt tgctggtgtt cgacgcgctc ggcaccaagt
    10501 tccgtacgct caagctgcgc aaggaccccg agtgccgggt ttgtagtaaa aagcccgagg
    10561 agatcgagct gatcgactac gaagcctttt gtagtctggc tcagtgatcg cctgacgacg
    10621 gatgtttggc gggtgatgcc tatgacgggg gtgggggggg
//
LOCUS       DASWNJ010001344         5170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_516963, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001344 DASWNJ010000000
VERSION     DASWNJ010001344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5170
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516963"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VGB85_22440"
     CDS             <1..434
                     /locus_tag="VGB85_22440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0136865.1"
                     /translation="LLSRVAGEAEPVATRNGTTVTVVAPPDLRVYCDRRRLETVVSEA
                     VGNACRFTRGGHVTLRASAEPDGARIEVEDDGPGMRPEDCERATTPFFQADGSSTRAH
                     DGAGLGLTVAARLCARMGGSLALRSAPGRGTTVTIHVPAHV"
     gene            625..1743
                     /locus_tag="VGB85_22445"
     CDS             625..1743
                     /locus_tag="VGB85_22445"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEY0136866.1"
                     /translation="MIERSRRLWIGLSWCVAAAGCNGGGAGSDGGTGTSTGMGSGITT
                     LTTGGAGDSEPTAGSDGGTTGMRLDLGEGSVATTETCADADVKFEAQTPTVLLLIDQS
                     GSMTENFGGTDRWDAVYDALLDQDDGLVPDLEDKIRFGMALYTSFDGNEGGECPVITA
                     TTPALNNYAAIEASYEAAKPEDETPTGDSIAAVTPTLVMDPSPGNKVIVLATDGEPDT
                     CAEPNPQNGQQASVDAAAAAFAQGVRLYVISVGAEVSDEHLQDVANAGVGVGQGDPNA
                     PFYKANDADALAAAFAEIVEGVRDCKLDVDGKVAEGGEAECVVSVNGEPVPYGTDDGW
                     QLNNPSEIELVGGSCDAIQDGDVAVSIKCACGVVTPVG"
     gene            complement(1900..3330)
                     /locus_tag="VGB85_22450"
     CDS             complement(1900..3330)
                     /locus_tag="VGB85_22450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEY0136867.1"
                     /translation="MSYSLLSVTYIAAAALFIMSLGGLSRQETARRGNLYGIIGMLLA
                     VLATAFGMNAQGLMNVSGTGFAMLAAVIIPGAVIGATLAARVAMTSMPELVAILHSFV
                     GAAAVLVGLASFLDPGAHPTGTEGTIHNVEIFVGVFIGAITFTGSVIAFLKLRGSIGG
                     KPLALPGRHLLNLAMILGSCFLGYQFLAGGHVDGLQPLLIMTGIACVIGIHMVMAIGG
                     ADMPVVVSMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCRAMNRSFLS
                     VIAGGFGSDGGVAAPKAIGGPVVDAKEMSLDEVVEALREARNVIVVPGYGMAVAQAQH
                     PVAEITKILKTRGVNFRFAIHPVAGRLPGHMNVLLAEANVPYDIVMEMDEINQDFPTT
                     DVVLVIGANDIVNPGAQDDPSSPIAGMPVLEVWKSRLVVVMKRGKGVGYAGVDNPLFY
                     KDCTRMLYGDAKKNVDALLTRLRDSA"
     gene            complement(3342..4904)
                     /gene="pntA"
                     /locus_tag="VGB85_22455"
     CDS             complement(3342..4904)
                     /gene="pntA"
                     /locus_tag="VGB85_22455"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - NAD(P)+ transhydrogenase
                     (AB-specific) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEY0136868.1"
                     /translation="MKIGIPKEIHPGERRVAATPDTAKRLKSFGFDVLVETGAGVAAE
                     FVDTAYTAVGCEIAADAPSLWASADLVLKVRPPTLDEVGLLREGGTLVSFIYPAQNKQ
                     LVEALAARRATVIAMDAVPRISRAQKLDALSSMANIAGYRAIVEAANNFGRFFQGQIT
                     AAGKVPPAKVLVIGAGVAGLAAIGVAKGLGAIVRSFDTRPVVKEQIESMGAEFLLLDF
                     AEDGTGDGGYAKPMSDEFIAAEMELFRKQAKEVDIIVTTALIPGRPAPKLITHDMLAL
                     MRPGSVVVDLAAEAGGNCEATVPGEKVTYGGVTIIGYTDLPSRMASQSSQLYGSNLCH
                     LLDDMGKAANYRVDEKDEVVRGALILRDGELKWPAPKPAAPLPQTQPIASIKPMPNKP
                     AQRGHGGGHHGPPAGEPGGPLPLLLAGAALIGVGVGAPPAFLSHFTVFVLACFIGYQV
                     VWNVAPALHTPLMSVTNAISGIIILGGMLQVSGAPTSAATILGAAAILLATINIAGGF
                     LVTQRMLKMFRR"
BASE COUNT          785 a         1676 c         1983 g          726 t
ORIGIN      
        1 cgctgctgag ccgggtcgcc ggcgaggcgg agccggtggc gacgcgcaac ggcacgacgg
       61 tcacggtcgt ggcgccgccg gacctgcggg tgtactgcga ccggcggcgg ctcgagacgg
      121 tggtgtcgga ggcggtcggc aacgcgtgtc gattcacgcg cggcggtcac gtcacgctgc
      181 gcgcgagcgc ggagccggac ggggcgcgca tcgaggtcga ggacgacggg ccgggcatgc
      241 gcccggagga ctgcgagcgg gcgacgacgc cgttctttca ggccgacggc agctcgacgc
      301 gagcgcacga cggggccggc ctgggcctga cggtggcggc ccgcctgtgc gcgcgcatgg
      361 gtggctcgct ggcgctgcgc agcgccccgg gccgcggcac gacggtgacg atccacgtgc
      421 cggcgcacgt gtgagcacct ggccggaggg acaggctcgg tgatggttcg agaacgagcg
      481 accgacgagc ggtcctgcac cacgctgtcc acgcaggtga cagcccggcc agcgtgacgc
      541 gcgtgcaccg gcgagcgcgc gcccgggggt gcggcccggt gattccacgc tggcagatgg
      601 ccgcggctgg ggcatcctgg gcgaatgatc gagcgttcgc ggagattatg gatcggtttg
      661 tcgtggtgcg tcgcggcggc gggctgcaac ggtggcggtg cggggtccga cggcggcacc
      721 ggtacgagca cgggcatggg ctcgggcatc acgaccttga cgaccggcgg agccggcgac
      781 agcgagccga ctgcgggcag cgacggcggc acgaccggga tgcgcctcga cctcggtgag
      841 ggcagcgtgg cgaccacgga gacctgcgcc gacgccgacg tgaagttcga ggcccagacg
      901 ccgacggtgc tgctgctgat cgatcagtcc ggcagcatga ccgagaactt cggcggcacc
      961 gaccgctggg acgccgtgta cgacgcgctg ctcgaccagg acgacgggct cgtgcccgat
     1021 ctcgaggaca agatccgctt cggcatggcg ctgtacacca gcttcgacgg caacgagggc
     1081 ggcgagtgcc cggtgatcac cgcgacgacc ccggcgctca acaactacgc cgcgatcgag
     1141 gcgagctacg aggcggccaa gcccgaggac gagacgccga ccggtgactc gatcgccgcg
     1201 gtcacgccga cgctggtgat ggacccgtca ccgggcaaca aggtcatcgt gctggcgacc
     1261 gacggcgagc ccgacacctg cgcggagccc aacccgcaga acggtcaaca ggcgtcggtc
     1321 gacgcggccg cggcagcgtt cgcgcagggc gttcgcctgt acgtgatcag cgtcggcgcg
     1381 gaggtcagcg acgagcacct gcaagacgtc gccaacgcgg gcgtcggcgt cggtcagggc
     1441 gacccgaacg cgccgtttta caaggccaac gacgcggacg cgctggcggc cgcgttcgcc
     1501 gagattgtcg agggggtgcg cgactgcaag ctcgacgtcg acggcaaggt cgccgagggc
     1561 ggcgaggccg agtgcgtggt gtcggtcaac ggcgagcctg tcccctacgg caccgacgac
     1621 ggctggcagc tcaacaaccc cagcgagatc gagctcgtcg gtgggtcgtg cgacgcgatc
     1681 caggacggcg acgtcgcggt gtcgatcaag tgcgcttgcg gcgtcgtcac cccggtggga
     1741 tgatcgacgc atgcggctcg cgtggttgcc ggtcgtgctt gcgagcgtcg ggtgcacgca
     1801 gtccgggccg cggcgcgagc cggtgccgtt cgcggcgtgc cccggagacg gggggccggg
     1861 gctggggaac cggcctgacg ccgatccggg agccgtgact cacgcgctgt cgcgcaggcg
     1921 ggtgagcagg gcgtcgacgt tcttcttggc gtcgccgtag agcatgcgcg tgcagtcttt
     1981 ataaaatagc gggttgtcga cgccggcgta gccgacgccc ttgccgcgct tcatgacgac
     2041 gacgagccgc gacttccaga cctcgagcac cggcatgccg gcgatcgggc tgctcgggtc
     2101 gtcctgggcg ccggggttga cgatgtcgtt ggcgccgatg acgaggacga cgtccgtggt
     2161 cgggaagtcc tggttgatct cgtccatctc catgacgatg tcgtaaggga cgttcgcctc
     2221 ggcgagcagg acgttcatgt ggccgggcag gcggccggcg accgggtgga tcgcgaagcg
     2281 gaagttgacg ccgcgcgtct tcaggatctt ggtgatctcg gccaccggat gctgggcctg
     2341 ggcgaccgcc atgccgtagc cgggcacgac gatgacgttg cgggcctcgc ggagcgcctc
     2401 gacgacctcg tcgagcgaca tctccttggc gtcgacgacc gggccgccga tcgccttggg
     2461 ggccgcgacg ccgccgtcgc tgccgaagcc gccggcgatg acgctcagga acgagcggtt
     2521 catcgcgcga cacatgatgt acgagaggat cgcgccgctg ctgccgacga gcgcgccggt
     2581 gacgatcagc aggtcgttcg acagcatgaa gccggccgcg gccgcggccc agccggagta
     2641 gctgttgagc atcgagacga cgacaggcat gtcggcgccg ccgatcgcca tcaccatgtg
     2701 gatgccgatc acgcaggcga tgccggtcat gatcagcagc ggctggaggc cgtcgacgtg
     2761 gccgccggcg aggaactggt agccgaggaa gcagctgccg aggatcatcg ccaggttgag
     2821 caggtggcga cccggcagcg cgagcggctt gccgccgatg ctgccgcgca gcttcaggaa
     2881 ggcgatgacg gagccggtga aggtgatcgc gccgatgaac acgccgacga agatctcgac
     2941 gttgtggatc gtgccctcgg tgccggtcgg gtgggcgccg gggtcgagga aggaggcgag
     3001 accgacgagc accgccgcgg cgccgacgaa gctgtgcagg atcgccacca gctcgggcat
     3061 cgaggtcatg gcgacccggg cggccagcgt cgcgccgatg accgcgccgg ggatgatcac
     3121 cgccgcgagc atggcgaagc cggtgcccga gacgttcatc aggccctgcg cgttcatgcc
     3181 gaacgcggtg gcgagcacgg cgagcagcat gccgatgatg ccgtagaggt tgccgcggcg
     3241 cgccgtctcc tggcgcgaga ggccgcccag gctcatgatg aagagcgcgg cggcggcgat
     3301 gtaggtgacg gagagaaggc tgtaggacac ggtctagaac ctcacctgcg gaacatcttg
     3361 agcatgcgct gggtgacgag gaagccgccc gcgatgttga tcgtcgccag caggatcgcc
     3421 gcggcgccga ggatcgtcgc cgcggaggtc ggcgcgcccg agacctgcaa catgccgccg
     3481 aggatgatga tgccgctgat cgcgttggtg acgctcatca gcggggtgtg cagcgccggc
     3541 gcgacgttcc agacgacctg gtagccgatg aagcaggcga gcacgaacac ggtgaagtga
     3601 ctgaggaacg ccggcggcgc gccgacgccg acgccgatca gcgccgcgcc tgccaacaac
     3661 agcggcagcg gaccgccggg ctcgcccgcg ggtgggccgt gatgtccgcc gccgtggccg
     3721 cgctgggccg gcttgttcgg catcggcttg atggacgcga tcggctgggt ctgcggcagc
     3781 ggggcggccg gcttgggcgc gggccacttg agctcaccgt cgcgcaggat cagcgcgccg
     3841 cggacgacct cgtccttctc gtcgacgcga tagttggcgg ccttgcccat gtcgtcgagc
     3901 aggtggcaca agttgctgcc gtagagctgg ctcgactggc tggccatgcg gctcggcagg
     3961 tcggtgtagc cgatgatcgt cacgccgccg taggtgacct tctcgccggg tacggtcgcc
     4021 tcgcaattgc caccggcctc ggccgcgagg tcgacgacga cggagcccgg gcgcatcagc
     4081 gcgagcatgt cgtgggtgat cagcttgggc gcggggcggc cggggatcag cgcggtggtg
     4141 acgatgatgt cgacctcctt cgcctgcttg cggaacagct ccatctccgc ggcgatgaac
     4201 tcgtcgctca tcggcttggc gtagccgccg tcgccggtgc catcctcggc gaagtcgagc
     4261 agcaggaact cggcgcccat gctctcgatc tgctccttga cgaccggccg ggtgtcgaag
     4321 gagcggacga tcgcgccgag gcccttggcg acgccgatcg cggcgaggcc ggccacgccg
     4381 gcgccgatca ccagcacctt ggcgggcggg accttgccgg cggcggtgat ctggccctgg
     4441 aagaagcggc cgaagttgtt ggcggcctcg acgatcgcgc ggtagcccgc gatgttggcc
     4501 atcgacgaga gtgcatcgag cttctgcgcc cgcgagatgc gaggcaccgc gtccatcgcg
     4561 atcacggtgg cgcgccgagc agcgagggct tcgacgagct gcttgttctg cgcggggtag
     4621 atgaagctga cgagcgtgcc gccctcgcgc agcaggccga cctcgtcgag cgtcggcggg
     4681 cggaccttga gcacgaggtc ggcgctggcc cacaggctcg gcgcgtccgc tgcgatctcg
     4741 cagccgacgg cggtgtaagc ggtgtcgacg aactccgccg cgacgccggc tccggtctcg
     4801 acgaggacgt cgaagccgaa gctcttgagg cgcttggcgg tgtcaggcgt ggctgccacc
     4861 cgacgctcgc ccgggtggat ctccttgggg atgccgatct tcatggtttt ggggacctgc
     4921 aggcgcgcgc agggaccagg tgaccctgga gccgcgcccc tggatatcac ggcggcacgg
     4981 ggggtgcata ccccatggaa gctggcgtac ggtggtccgg atgacggacc ccctcaacca
     5041 cttcgacccg ggcggcgcgg cccacatggt ggcggtgacg ggcaagccgg agaccccacg
     5101 cggcgcggtg gcccgggccc ggatccgcgt cgcacctgcc accttcgcgc ggctctccgg
     5161 cggtacggtc
//
LOCUS       DASWNJ010001345         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_516972, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001345 DASWNJ010000000
VERSION     DASWNJ010001345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516972"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..744)
                     /locus_tag="VGB85_22460"
     CDS             complement(148..744)
                     /locus_tag="VGB85_22460"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEY0136869.1"
                     /translation="MTTRDDLGATLVQLRSRVDAHFDAAVARSPHAFRCGEGCDACCH
                     VRIGVFAVEAAPIREALARLAREDPALRERVRMQADDPGHRDRCALLVEGRCAVYEQR
                     PLICRSHGLPVLAADPGAAPGAALQLDHCPLNFTDEAPPRASMLRLDAVNQPLSMLAR
                     LWTATDDAGRSPPDDDRIALEDLARAADPDEPGQKSPP"
     gene            complement(749..1729)
                     /locus_tag="VGB85_22465"
     CDS             complement(749..1729)
                     /locus_tag="VGB85_22465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136870.1"
                     /translation="MAVHVLHHGWCFDGAASSALFAAFWRQRFGAGQVLRYIPKDHRP
                     GDPYDPEDFAGADPAVGLDFRYSQHPAMTWYFDHHASAFQLPGDRAHFAADRSGQKFH
                     DGSAASCAGYIVRVVRERFGCDLSAHAELIGWAERIDAASFPSPEMPVLLVEPALQLA
                     SFVESNHDMALVATFIDDLRTRPLAEHAAADYVQKIVRPRRAQNQDDLALIASRAIVR
                     DGVLAYEVLDQPARAYNKFIPYYHHPRVRYVVALIRGSDRRIRLTAGYNPWLPREARE
                     HSMAALLEPHGGGGHPYVGGCSFDADQEERALAVQRSLVDILRGAAPAAS"
     gene            complement(1785..>2256)
                     /locus_tag="VGB85_22470"
     CDS             complement(1785..>2256)
                     /locus_tag="VGB85_22470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136871.1"
                     /translation="PAGRADEVEALRAGLRSTLPEFLIRSGGPIGDRELVVLSFLATL
                     HAAGELGKAYATPATIRKALVALDERLGHPADLNVMVSDGRSVGVLHRGGRLVHLTPP
                     PPPRPARAISQPHQRPTASLLLFDPHGASEAGDPDVPEGVFTVHVRQPGVHDRD"
BASE COUNT          311 a          736 c          903 g          306 t
ORIGIN      
        1 tgacctccgg gttatgagcc cggcgagcta cctggctgct ccaccccgca gcgaggaggc
       61 gatttgtact ctcgaccctt gggcccgtca accactttcg accgcgaata acatcgcgcg
      121 agttccgcgg tttggcccag aacacgctca gggcggcgat ttttgcccgg gctcgtccgg
      181 gtcggctgcc cgggccagat cttcgagggc gatccggtcg tcgtcgggcg gagatcttcc
      241 ggcgtcgtcc gtggcagtcc acagccgggc gagcatcgag agcggctgat tgacggcgtc
      301 gagccgcaac atactcgcac gcggcggggc ctcgtcggtg aagttgaggg ggcagtggtc
      361 gagctgcagc gcggcgcccg gggcggcgcc gggatcggcg gcgagcacgg ggagcccgtg
      421 cgaccggcag atcagcgggc gctgctcgta gacggcgcag cgaccttcga ccaacagggc
      481 gcagcggtcc cggtgccccg ggtcgtcggc ctgcatgcgc acgcgctcgc gcagggccgg
      541 gtcctcgcgg gcgaggcggg cgagggcctc gcggatcggg gcggcctcga cggcgaacac
      601 accgatgcgc acgtggcagc aggcgtcgca gccctcgccg cagcgaaacg cgtgcgggct
      661 gcgggcgacc gcggcgtcga agtgggcgtc gacccggctt cgcagctgca cgagggtcgc
      721 gccgaggtcg tcgcgggtcg tcatctcgct aactcgccgc cggcgcggct ccgcggagga
      781 tgtcgaccag gctgcgctgg accgcgaggg cgcgctcctc ctggtcggcg tcgaagctgc
      841 aaccgccgac gtaaggatgg ccaccgccgc cgtggggctc gagcagggcg gccatgctgt
      901 gctcgcgggc ctcgcggggc agccacgggt tgtagccggc ggtcaggcgg atcctgcgat
      961 cggagccgcg gatcagggcg acgacgtagc gcacgcgcgg gtgatgatag taggggatga
     1021 acttgttgta cgcgcgggcc ggctggtcga ggacctcgta ggcgagcacg ccgtcgcgca
     1081 cgatcgcgcg cgaggcgatc agggcgaggt cgtcctggtt ctgggcccgg cgcgggcgga
     1141 cgatcttctg cacgtagtcg gcggcggcgt gctcggcgag cggccgggtg cgcaggtcgt
     1201 cgatgaaggt ggcgacgagg gccatgtcgt ggttgctctc gacgaagctg gccagctgca
     1261 gcgccggctc gacgagcaac accggcatct cggggctcgg gaagctggcg gcgtcgatgc
     1321 gctcggccca gccgatcagc tcggcgtggg ccgagaggtc gcagccgaag cgctcgcgga
     1381 cgacgcggac gatgtagccg gcgcagctcg ccgccgaccc gtcgtggaac ttctggccgc
     1441 tgcggtcggc cgcgaagtgg gcccggtcgc ccgggagctg gaaggcgctg gcgtggtggt
     1501 cgaagtacca ggtcatcgcc gggtgctggc tgtaacggaa gtcgaggccg accgccgggt
     1561 cggcgccggc gaagtcctcg gggtcgtagg ggtcgccagg acggtggtcc ttggggatgt
     1621 agcgcaggac ttgtccggcg ccgaagcgct ggcgccagaa agcggcgaac agcgccgacg
     1681 aggcggcgcc gtcgaagcac cagccatgat ggaggacgtg gactgccatg agctcgcccg
     1741 cgggttatac ccgcgccttc cggccacgtc tcgaaaccgg cgggtcagtc ccggtcgtgc
     1801 acgcccggct ggcggacatg gacggtgaac acgccctccg ggacgtccgg gtcgccggcc
     1861 tcgctggcgc cgtgggggtc gaacagcagc aggctggcgg tcgggcgctg gtgcggctgc
     1921 gagatggcgc gagccggccg cggcgggggc gggggggtca agtggaccag gcggccgccg
     1981 cgatggagca cgccgacgct gcgaccgtcg ctgaccatga cattgaggtc ggccgggtgg
     2041 ccaaggcgct cgtcgagcgc gaccagggcc ttgcggatcg tcgccggggt cgcgtaggcc
     2101 ttgccgagct caccggccgc gtggagcgtc gccaaaaagg acagcaccac gagctcgcga
     2161 tcgccgatcg gtccgccgct gcggatcagg aactccggga gggtcgagcg cagtccggcg
     2221 cgcagggcct cgacctcgtc cgcgcgcccg gccggc
//
LOCUS       DASWNJ010001346         1516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_406492, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001346 DASWNJ010000000
VERSION     DASWNJ010001346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406492"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VGB85_22475"
     CDS             <1..300
                     /locus_tag="VGB85_22475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136872.1"
                     /translation="RLPGGEVHPFVGYRRDAGLAYGHFKLGERAAALAYAQSALEHAG
                     DGGYVRLRAMGLGLLARILGEQAGAAARARALGIARRLEDEELLGRIEARHAPEV"
     gene            458..1051
                     /locus_tag="VGB85_22480"
     CDS             458..1051
                     /locus_tag="VGB85_22480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136873.1"
                     /translation="MSEHSLIEVDRRATCAAEAEARLQERDPVAAEALLRGLSIEDPS
                     GRLSALLGDACFLQGRYEQAAAAYLEALHNVPGTSDWEAAAKRAWENAALHRVGEAVG
                     GALANVAGGALYALMEAFGRAGIAEEVWTHWYRDLEGVPGAVDEAIVIFKLAYMRREL
                     FANNIVPTYLDDAAAGCLHASDRAAGSVCGRSAASTP"
     gene            1261..>1516
                     /locus_tag="VGB85_22485"
     CDS             1261..>1516
                     /locus_tag="VGB85_22485"
                     /inference="COORDINATES: protein motif:HMM:NF037747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HEY0136874.1"
                     /translation="MANATSKPGRASKNIERVEFPELTWKSDGDVANGLTSLYKHAEA
                     RAESTIAWYLRAKASKSFNSRALRLLTILLGTLGGLAPILD"
BASE COUNT          227 a          508 c          580 g          201 t
ORIGIN      
        1 cggttgcccg gcggcgaggt ccatccattc gtcggctatc gccgcgacgc cgggctggcg
       61 tacgggcact tcaagctcgg tgagcgcgcc gcggccctcg catatgcaca gtcggcgctc
      121 gagcacgcgg gcgacggcgg ctatgtccgc ctgcgtgcga tgggcctcgg cctgctcgcg
      181 cggatcctcg gcgagcaggc cggcgccgcg gcgcgtgcac gggccctggg catcgcccgc
      241 cggctcgagg acgaggagct gctcgggcgc atcgaggccc gccacgcgcc cgaagtgtga
      301 tgatcgatca cctcgaccca tgcgtgcagc gacacgaatg cgcgcacccg gacagcgtac
      361 ggatgtcgcg cgaaaggact gcgcagtcgg gctgcccgcg aagccgttgc ctcgcccacg
      421 gaccctgcgg taatgtgaca gcggtttgga ggggtacatg agcgagcatt cgctgatcga
      481 ggtggatcgc agggcgacgt gcgcggccga ggccgaggca cggctgcaag agcgcgaccc
      541 ggtggccgcg gaggcgctgc tgcgtgggct gagcatcgag gacccgagcg ggcgcctgtc
      601 ggcgctgctc ggcgacgcgt gcttcctgca aggccgctac gagcaggcgg cggcggcgta
      661 cctcgaggcg ctgcacaacg tacccgggac gtcggactgg gaggccgcgg cgaagcgcgc
      721 ctgggagaac gcggcgctgc atcgcgtcgg cgaggcggtc ggcggcgcgc tggcgaatgt
      781 cgccggcggg gccctgtacg cgctcatgga ggccttcggc cgcgccggga tcgccgagga
      841 ggtgtggacc cactggtacc gcgacctcga gggcgtgccc ggggccgtcg atgaggcgat
      901 cgtcatcttc aagctcgcgt acatgcgccg tgagctgttc gccaacaaca tcgtgcccac
      961 atacctcgac gacgcggccg cggggtgcct gcatgcgagc gatcgcgccg cgggctcggt
     1021 ctgtggccga tcggcagctt cgacgccttg accgaggacg ccgagcaggt cgcgagctgc
     1081 gcgaggtaac ggtcagacgg acggccgcga cgacgtcgcg ggttcgtgag gcgggcaagc
     1141 ccacgagcgc gtagcgggcg cggccgcaca gcagcgccag catacgcgcc gggcccggcg
     1201 aagctcccac gccggcgcgg tagtttcgcc cgcgggccga agtgctatcc tggcgcggtc
     1261 atggcgaacg cgaccagcaa gccggggaga gcctcgaaga acatcgaacg ggtcgagttc
     1321 cccgagctga cgtggaagtc ggacggcgac gtcgccaacg gtctgacgag cctctacaag
     1381 cacgccgagg cgcgcgccga gtcgacgatc gcgtggtacc tgcgggccaa ggcgagcaag
     1441 tcgttcaact cgcgggcgct gcggctgctg acgatcctgc tgggcacgct cggtgggctc
     1501 gcgccgatcc tcgact
//
LOCUS       DASWNJ010001347         1812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_406511, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001347 DASWNJ010000000
VERSION     DASWNJ010001347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406511"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1433
                     /locus_tag="VGB85_22490"
     CDS             171..1433
                     /locus_tag="VGB85_22490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEY0136875.1"
                     /translation="MTNLLVFLLVVSVLAACGLIQIDKIVSGTGKLTADAPNILLRPF
                     DQAIVESIDARKGDIVRKGQVLARLNSTFSSADLTTLQDQVDLLSAEVMRLQSEFANS
                     TYKPDPSNPHAKLQASIFGQRSSEYKSSLQNYDQTINQLKAQIAGDSTQAKYYRERLS
                     LASKNEQMRQRLQDLEIGSTLNTQIATDARLNMASALAQAETDGAQAGSKLAAQKAER
                     ETFVQRWKGQTSQELADTRRRLVQAQQEFAKTSLRNNLVTMTAPRDSVVLSVAQVTAG
                     SVVSSAEPLIHLVPIDAPLSVEVDISGIDSGYVRPGDEVTIKFDTLPYLQYGTGRGVV
                     RSISADSFNPESTAQEGGSALPNRPSSLYYKGDIALAELLLHDTPPGFRLMPGMPVAA
                     DVKVGTRSVLSYFIARILPVVYGSMHEP"
     gene            1446..>1812
                     /locus_tag="VGB85_22495"
     CDS             1446..>1812
                     /locus_tag="VGB85_22495"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136876.1"
                     /translation="MTRPNILARSLDRLASVSPKRARVRAERLAETAPERALALFAVA
                     AEAGDARAAFAVGEGFLEGKGTPRDPVEAARWYHRAAAAGHGRAQCRLAQLHLFGLAG
                     PAVGRNAGLFEPVATGGGGA"
BASE COUNT          294 a          695 c          584 g          239 t
ORIGIN      
        1 ccaggacaag atccgcgtcg cccccgcccc cgcactcgcc ctctccccga ggctcgcgca
       61 gagtggctag ggccggaccg aggggcacga ccccgctccc gctggccgct ctcgagttcg
      121 agagcccgtc ggcggcgatc atcgccaccg cggtgccccc gctgtcgcgc gtgaccaacc
      181 tcctcgtctt cctgctggtc gtctccgtgc tcgccgcctg cgggctgatc cagatcgaca
      241 agatcgtctc gggcaccggc aagctgaccg ccgacgcgcc caacatcctc ctgcggccct
      301 tcgaccaggc catcgtcgag agcatcgacg cgaggaaggg cgacatcgtc cgcaagggcc
      361 aggtgctggc ccgcctcaac tccaccttca gctccgccga cctcaccacc ttgcaagacc
      421 aggtcgacct gctcagcgcc gaggtcatgc gcctgcagtc cgagttcgcc aactcgacct
      481 acaagcctga cccgagcaat cctcacgcca agctgcaggc ctcgatcttc ggtcaacggt
      541 ccagcgaata caaatcgtcc ctgcagaact acgaccagac catcaaccag ctgaaggcgc
      601 agatcgcggg cgatagcacg caggccaagt attatcgcga gcgtctcagc ctcgcctcca
      661 agaacgagca gatgcgccag agattgcagg atctcgagat cggcagcacg ctcaacaccc
      721 agatcgccac cgacgcccgg ctgaacatgg cgtccgcgct cgcccaggcg gagacggacg
      781 gcgcccaggc gggcagcaag ctggccgccc agaaggccga gcgcgagacc ttcgtccagc
      841 gctggaaggg ccagacctcg caggagctcg ccgacacgcg ccgccggctg gtgcaggccc
      901 agcaggagtt cgccaagacc agcctgcgca acaacctcgt gacgatgacg gcgccgcgcg
      961 actcggtcgt gctgtcggtc gcccaggtca ccgcgggctc ggtcgtcagc agcgccgagc
     1021 cgctgatcca cctcgtgccg atcgacgcgc ccctctcggt cgaggtggac atctcgggca
     1081 tcgacagcgg ctacgtccgc ccgggcgacg aggtgacgat caagttcgac acgctgccct
     1141 acctgcaata cggcaccggg cgcggcgtcg tgcgctcgat cagcgccgac agcttcaacc
     1201 ccgagtccac ggcgcaggaa ggcggctccg cgctgccgaa ccggccgagc tcgctctact
     1261 acaagggcga catcgccctc gccgagctgc tgctgcacga cacgccgccg ggcttccgcc
     1321 tgatgccggg catgccggtc gccgcggacg tgaaggtcgg gacgcgctcc gtgctcagct
     1381 acttcatcgc caggatcctg ccggtcgtct acggcagcat gcacgagccc tgagccgcca
     1441 accgcatgac ccgcccgaac atcctcgcca ggtcgctcga ccgcctggcc tccgtctcgc
     1501 cgaagcgggc gcgggtgcgc gccgagcgcc tcgccgagac ggcgccggaa cgcgcgctcg
     1561 cgctgttcgc ggtggcggcc gaggcgggcg acgcccgggc ggccttcgcc gtcggcgaag
     1621 gcttcctcga aggcaagggc acgccgcgcg acccggtcga ggccgcgcgc tggtaccatc
     1681 gcgccgccgc ggcggggcac ggtcgcgcgc agtgccgcct agcccagctc cacctcttcg
     1741 gcctagctgg gcccgcggtc gggcggaacg ccgggctgtt cgagcccgtc gcgacgggag
     1801 ggggtggcgc gg
//
LOCUS       DASWNJ010001348         4029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_555597, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001348 DASWNJ010000000
VERSION     DASWNJ010001348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555597"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1681
                     /locus_tag="VGB85_22500"
     CDS             <1..1681
                     /locus_tag="VGB85_22500"
                     /inference="COORDINATES: protein motif:HMM:NF024220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0136877.1"
                     /translation="DPGEACDDGKNGDQDDGCTDSCKAPVCGDSFVQMSLGEQCDLGG
                     ANNNTGECTLICKNAVCGDSLIQEGVEGCDDGANNGNNKACKADCSPNFCGDGFVGPG
                     EACDDGNQSNDDACTNVCKQATCGDGFKQPGEECDLGNLNNNAGACTLACKLPKCGDQ
                     LVQPLTEQCDDGNLDNTDLCLSTCKDAKCGDAFVQAGVEACDDGNQLNTDTCTNMCKA
                     AKCGDAFVQASNGETCDDGNLVNTDACTGMCKMAACGDAIVWQGVETCDDGNQLNNDA
                     CVMCKPAKCGDGFAQMGVEECDDGNLANGDGCTAMCKKEAVVKTFACTKAAQSFVVPQ
                     GVTKIKIESWGAQGNYPTNNDGGVCGGVPDIGGRGGYVTGDLTVVPGETLQINVGCMG
                     GKGNLPGWNGGGGSCADPSTCSSGGGGSDVRKGGTGLANRVLVAGGGGGAEYSCGGQG
                     GGHGGGLTGNTGLGSDFDSASGGGGTQGAGGAAGISYTIAQNGSPGTLGQGGSGSTEQ
                     HAGGGGGGYWGGGGGGVDGHAGGGSSYIGGVANGSTQVGLKTGDGQIVLSY"
     gene            complement(1774..3534)
                     /locus_tag="VGB85_22505"
     CDS             complement(1774..3534)
                     /locus_tag="VGB85_22505"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEY0136878.1"
                     /translation="MITRTTTGGVMRGALVLAALGLACGPGAPGADTGEASTGAAGSD
                     GSSEPTGAPPTTATVTTADSEATTPTSDGTDGGDETGTPPAACPPGPSTPQRLSNLQY
                     INTLTDLFPGVDLPPLDLLPNNAVEGFENIADANPDHPDLLAKFGAAADAVATAATQQ
                     GGPWLPCPPDGGADPEACGHAVLPGLVARMFRRPLTAEEETDLLAHFDGQLQEGSFAG
                     ALHLELATRLASDEFLHLRNTGGTPVEGQPGVLARDGFDMAARMAYFLWDTTPDAPLL
                     ALAAGGGLDTAEGIAAQVAAMQADPRAAAGLARFARQWLYLHALDDAAGLPENLPPEL
                     QEPLRLDIDRFVVGVLHDGEGTLAALLTSPVAFVTPWTANFYDVAQKEAQVELDPERR
                     PGLLTRVGWLALRSTSTHHSPFKRGWKLADSLLCSQLPPPPPDVEATLPDDPGPDATT
                     RQLYDTILAQPQCAGCHEPMHRIGYGFEHYDLFGAWQDQEKMQAVDASGEVSAFFGSD
                     IGGPFEGAAELTARLAGSRSVHDCVTRKTLMYALGRTLADIDACTLESLQAQFFAGGG
                     DFEALQAAIILSDGFRRRAE"
     gene            3648..>4029
                     /locus_tag="VGB85_22510"
     CDS             3648..>4029
                     /locus_tag="VGB85_22510"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY0136879.1"
                     /translation="MDAPEYTIDELAALTRVPTRTIRFYQTHKALMPPVIRGRVAYYG
                     PAHRERLERIAQLQDRGLRITAIRDLLGSIDRGEVGLAEWLGVEREVAASWVEDRPRT
                     LTAAELYELAGSRRPGLLADLARSG"
BASE COUNT          597 a         1435 c         1475 g          522 t
ORIGIN      
        1 cgaccccggc gaggcctgcg acgacggcaa gaacggcgac caggacgacg gctgcaccga
       61 tagctgcaag gccccggtct gcggtgacag cttcgttcag atgagcctcg gcgagcagtg
      121 cgacctcggc ggcgccaaca acaacaccgg cgagtgcacg ctcatctgca aaaacgcggt
      181 gtgcggcgac agcctgatcc aggagggcgt cgagggctgc gacgacggcg cgaacaacgg
      241 caacaacaag gcctgcaagg ccgactgctc gcccaacttc tgcggcgacg gcttcgtcgg
      301 cccgggcgag gcctgcgacg acggcaacca gagcaacgac gacgcctgca ccaacgtctg
      361 caagcaggcc acctgcggcg acggcttcaa gcagcccggc gaggagtgcg acctcggcaa
      421 cctcaacaac aacgccggcg cgtgcacgct cgcctgcaag ctgcccaagt gcggcgacca
      481 gctggtccag ccgctgaccg agcagtgcga cgacggcaac ctcgacaaca ccgacctgtg
      541 cctcagcacc tgcaaggacg ccaagtgcgg cgacgcgttc gtgcaagccg gcgtcgaggc
      601 ctgcgacgac ggtaaccagc tcaacacgga cacctgcacc aacatgtgca aggcggccaa
      661 gtgcggcgac gccttcgtcc aggcgagcaa cggcgagacc tgcgacgacg gcaacctggt
      721 caacaccgac gcgtgcacgg ggatgtgcaa gatggccgcc tgcggcgacg cgatcgtgtg
      781 gcagggcgtg gagacctgcg acgacggcaa ccagctcaac aacgacgcct gcgtgatgtg
      841 caagccggcc aagtgcggcg acggcttcgc gcagatgggc gtcgaggagt gcgacgacgg
      901 caacctcgcc aacggcgacg gctgcaccgc gatgtgcaag aaggaggcgg tcgtcaagac
      961 cttcgcctgc accaaggcgg cgcagagctt cgtcgtcccg cagggggtca cgaagatcaa
     1021 gatcgagagc tggggcgcgc agggcaacta cccgaccaac aacgacggcg gcgtctgtgg
     1081 cggcgtcccg gacatcggcg gccgcggcgg ctatgtgacc ggcgacctca ccgtggtccc
     1141 cggtgagacg ctgcagatca acgtcggctg catgggcggc aagggcaacc tgccgggctg
     1201 gaacggcggc ggcggcagct gcgccgatcc gagcacctgc tcgagcggcg gcggcggatc
     1261 cgacgtgcgc aagggcggca ccggcctcgc caaccgcgtc ctcgtcgcgg gcggcggcgg
     1321 tggtgcggag tactcgtgcg gcggtcaggg cggcggccac ggcggtggcc tgaccggtaa
     1381 caccggcctc ggcagcgact tcgacagcgc ctccggcggc ggcggcacgc agggtgcggg
     1441 cggcgcggcc ggcatcagct acacgatcgc gcaaaacggc tcccccggca cgctgggtca
     1501 gggcggctcc ggctcgaccg agcagcacgc cggtggcggc ggtggcggtt actggggcgg
     1561 cggcggcggc ggtgtcgacg gccacgctgg cggcggctcg tcgtacatcg gcggggtcgc
     1621 caacggctcg acgcaggttg gtctcaagac cggcgacggc cagatcgtgc tcagctactg
     1681 atcaagtgag ccacgcggcg ccacgcggcg cccgaacccc acgcgcccgg tcgctgtcct
     1741 ttcggacagc ctccgggcgc gcccgttttc tagctactcg gcgcggcggc gaaagccgtc
     1801 gctgaggatg atcgccgcct gcagcgcctc gaagtcgccg ccgccggcga agaactgcgc
     1861 ctgcaacgac tcgagcgtgc acgcgtcgat gtcggcgagc gtgcgcccga gcgcgtacat
     1921 cagcgtcttg cgggtcacgc agtcgtgcac gctgcggctg cccgcgagcc gcgcggtgag
     1981 ctcggcggcg ccttcgaacg gcccgccgat gtcgctgccg aagaaggccg agacctcgcc
     2041 gctggcgtcg accgcttgca tcttctcctg gtcctgccag gcgccgaaca ggtcgtagtg
     2101 ctcgaagccg tagccgatcc ggtgcatcgg ctcgtgacag ccggcgcact gcggctgggc
     2161 gaggatcgtg tcgtacagct ggcgcgtcgt cgcgtccggg ccggggtcgt cgggcagcgt
     2221 cgcctcgacg tccggcggtg gcggcggcag ctgcgagcac agcaggctgt ccgcgagctt
     2281 ccagccgcgc ttgaacggcg agtggtgggt gctcgtcgag cgcagcgcga gccagccgac
     2341 gcgcgtcagc agcccggggc ggcgctccgg atcgagctcg acctgcgctt ctttctgcgc
     2401 cacatcgtag aagttggccg tccagggggt cacgaacgcg accggcgagg tcagcagcgc
     2461 cgcgagcgtg ccctcgccgt cgtgcagcac gccgacgacg aagcggtcga tgtcgaggcg
     2521 cagcggttct tgcagctccg gcggcaggtt ctcggggagc cccgccgcgt catcgagcgc
     2581 gtgcagatac agccactgtc gcgcgaagcg ggccagcccc gcggccgcgc gcgggtcggc
     2641 ctgcatcgcg gcgacctgcg cggcgatgcc ctccgcggtg tcgagcccgc ccccggccgc
     2701 gagcgcgagc agcggggcgt cgggcgtcgt gtcccacagg aagtaggcca tgcgcgcggc
     2761 catgtcgaag ccgtcgcgcg cgagcacccc cggctggccc tcgaccggcg tgccgccggt
     2821 gttccgcagg tgcaggaact cgtcggaggc gagccgggtc gccagctcga ggtgcagcgc
     2881 gccggcgaag ctgccctctt gcagctgtcc atcgaagtgc gcgagcaggt ccgtctcctc
     2941 ctcggcggtc agcgggcgcc ggaacatccg cgccacgagc cccggcagga ccgcgtgccc
     3001 gcaggcctcg gggtcggccc cgccgtccgg cggacacggc aaccacgggc cgccctgctg
     3061 ggtcgcggcg gtcgcgaccg cgtccgccgc ggcgccgaac ttggcgagca ggtccgggtg
     3121 gtccgggttg gcgtcggcga tgttttcaaa gccctcgacc gcgttgttcg gcaggaggtc
     3181 gagcggcggc agatcgacgc cggggaacag gtccgtgagc gtgttgatgt actgcaggtt
     3241 gctcaggcgc tgcggggtcg acggaccggg ggggcacgcg gccggcggcg tgcccgtctc
     3301 gtcgccaccg tcggtcccgt cgctcgtcgg cgtcgtcgcc tcgctgtccg ccgtggtcac
     3361 cgtggccgtc gtcggcggcg cgccggtcgg ctcgctgctg ccgtcgctcc cggccgcgcc
     3421 ggtgctcgcc tcgccagtgt cggcaccggg ggcacccggc ccgcacgcga ggcccagcgc
     3481 cgcgagcacc agcgcgccgc gcatcactcc acccgtcgtc gttcgcgtga tcatccgctg
     3541 tctccgtgtt gctggccacc gtacgccagc gtccgggctc cgacaatccg gcccgtggcc
     3601 cccagcgacc ttgacactcg caactgtaac ggttacagct gccacggttg gacgcgccgg
     3661 agtacacgat cgacgaattg gccgcgctga cgcgcgtgcc gacgcggacg atccgcttct
     3721 atcagaccca caaggctctg atgccgccgg tgatccgtgg gcgcgtcgcc tactacggtc
     3781 ccgcgcaccg cgagcggctc gagcgcatcg cccagctgca ggaccgcggc ctgcggatca
     3841 cggcgatccg cgacctgctc ggcagcatcg atcgcggcga ggtcgggctc gccgagtggc
     3901 tcggggtcga gcgcgaggtc gcggcctcgt gggtcgagga tcgcccgcgc acgctcacgg
     3961 ccgcggagct gtacgagctc gccggctcgc gccgcccggg gctgctcgcc gacctcgcgc
     4021 gcagcggcc
//
LOCUS       DASWNJ010001349         1646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_128972, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001349 DASWNJ010000000
VERSION     DASWNJ010001349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128972"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VGB85_22515"
     CDS             <1..230
                     /locus_tag="VGB85_22515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136880.1"
                     /translation="RGFTGFIFRHITRSSGGPRVSRIAKFFLVKDRKAFKEHLLRLAD
                     LPALQRMIVSHHLMVHDDAAGAIRQAAATLG"
     gene            400..1362
                     /locus_tag="VGB85_22520"
     CDS             400..1362
                     /locus_tag="VGB85_22520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136881.1"
                     /translation="MRTVELTIVLSIVASCIDTSTATDTGDTGTATTTTTSGTVGTPT
                     TSSGETETETETETETTSTGADESTGTTGAADICTPVVETIPEAEFVSTVINTICAQK
                     VECGCADDMCSVKFKADLEGVVKYAGDQGLVYDPSCAALAVHDYVTARGCMNGEQASY
                     PFCGSCPVFRGDTADGAACMDAEDFATFLFSQPCAGSESLCIDGTCAVPPSGTLKLGE
                     VCYSDEKTILLDCEPGTACDFFDSLRCEQELLEGDPCLVDDGVCGVDLVCGGKSVCAP
                     YLPPGEPCAYDYDCASFYCEGSVCTEYVWICDLTSINDLFGGLH"
     gene            1401..>1646
                     /locus_tag="VGB85_22525"
     CDS             1401..>1646
                     /locus_tag="VGB85_22525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136882.1"
                     /translation="MQDQVLAYFAGERQAGMLAALLGALALAAAGVLFQPRWELRSLA
                     VTLGLMGLLELTVGLGLLVRTGPQVSQLLAQLGADAAA"
BASE COUNT          255 a          549 c          568 g          274 t
ORIGIN      
        1 gccgcggctt caccggcttc atctttcgcc acatcacccg gagctccggc ggcccccgcg
       61 tatcgcggat cgccaagttc ttcctggtca aggaccgcaa ggcgttcaag gagcacctcc
      121 tgcgcctcgc cgacctgccc gccctgcagc gaatgatcgt atcgcatcac ctgatggtcc
      181 acgacgacgc cgccggcgcg atccgccagg ccgccgcgac gctcggctaa aaagcctgcg
      241 agctttcccg cgctccgggg tccggaccga tcatccgcgg ggaactacct atagcccatc
      301 cacgcggcag tgcggcggcg cgaggggcct ggcgccgaca cccgttcgcg cgccgttttg
      361 cgtgttgcga tgtgaaggct ccgcgagcat gctcccccga tgcggacggt cgagcttaca
      421 attgtattgt cgatcgttgc gagctgtatc gatacgtcga ccgcaaccga cacgggggac
      481 accgggacgg cgacgacgac gacgacgagc ggcacggtcg ggacgccgac gacgagcagc
      541 ggcgagaccg agaccgagac cgagaccgag accgagacca cgtcgaccgg cgccgacgag
      601 tcgaccggca cgacgggcgc ggccgacatc tgcacgccgg tggtcgagac gatccccgag
      661 gcggagttcg tgtcgaccgt catcaacacg atctgcgcgc agaaggtcga gtgcggctgc
      721 gccgacgata tgtgctcggt caagttcaag gccgacctcg agggcgtggt caagtacgca
      781 ggcgaccagg gcctggtgta cgacccctcg tgcgccgcgc tggccgtgca cgactatgtg
      841 acggcgcgcg ggtgcatgaa cggtgaacag gccagctatc cgttctgtgg cagctgcccg
      901 gtgtttcgtg gcgacacggc ggacggcgcc gcgtgtatgg acgcggagga cttcgcgacc
      961 ttcctgtttt cgcagccgtg cgccggctcg gagtcgctgt gcatcgacgg cacctgcgcc
     1021 gtgccgccct ccggtacgct caagcttggc gaggtgtgtt actcggacga gaagacgatc
     1081 ctcctggact gcgagcccgg gactgcgtgc gatttcttcg actccctgcg ctgcgagcaa
     1141 gagctgctcg agggcgaccc ttgcctcgtg gacgacggcg tctgcggcgt cgacctcgtc
     1201 tgcggcggca aatcggtgtg cgcgccgtat ctcccgccgg gggaaccgtg cgcgtacgac
     1261 tacgattgtg cgtcgtttta ctgtgagggc tccgtgtgca cggagtatgt gtggatctgc
     1321 gacctcacga gcatcaacga cctgttcgga gggttgcact gaccaaaacg accggctgac
     1381 ttcgtgcgac catgcgggcc atgcaggacc aggtgctcgc gtacttcgca ggagagagac
     1441 aggccggcat gctcgccgcg ctgctcggcg cgctcgcgct ggcggccgcg ggtgtgctgt
     1501 ttcagccgcg ctgggagctg cgctcgctgg cggtcacgct cgggctgatg gggctgctcg
     1561 agctcacggt cggcctcgga ctgctcgtgc gcaccgggcc gcaggtctcg cagctgctgg
     1621 cacagctcgg cgccgacgcg gcggcc
//
LOCUS       DASWNJ010001350         5102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_24981, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001350 DASWNJ010000000
VERSION     DASWNJ010001350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24981"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VGB85_22530"
     CDS             <1..720
                     /locus_tag="VGB85_22530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136883.1"
                     /translation="AGITALHYYDPAIGPWRISKQQAAVLFAVIVAVVPMLAIGGYLL
                     LRRRLPAWHRFVVQWLLGKRLWLGIGLLMHLGIDVLMNVGTFVQVMIAVYVAWLSGPE
                     LDTIWHTLMSRPQAPGTGERPRYKKRWQTLLRAPIDRLRFRVPRPAYVVHHNPGEDSV
                     RRAALLRVWDLGDRLSFVADDGLAPRRLCVEVPGVGLRCDADAGRALVTVLPGFWWMY
                     PWCLLPGLSRLCGWLVVRTFR"
     gene            878..2545
                     /locus_tag="VGB85_22535"
     CDS             878..2545
                     /locus_tag="VGB85_22535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136884.1"
                     /translation="MRPSTLVFLVAVAAAGCGDAPALASLTSAATETPHGSTSTGTDE
                     PGSTSETGTPGSDETGTSTTPSGASTTSDADSSTGQPALPKLLEATLTPEVITVEGSI
                     TAHALAQDADGVRMQVDGGAPVELDELAPGEFTGQLGVFTALSNGTHDVVFTAWDDST
                     ASEPLSRQYEAKLNPAGGESYWDASGVLGQGFGRGVAIDPATGAVYEFFDVKEGDGER
                     CHVRRRGAQGEYFEADLIAVLPEQQCTGTAISVAADGTLYVLAEGQLNGDATWWLGRK
                     DSWDAPMLTLAWGDPGEKAYAIAVHAERAEVAVCGTTLTDGTDFDELDGRVAIFRKDM
                     PGYEPAPFDYTANQIGMSHFFDETLRDCAYDGDTLVLAGEANGKHPDNLFVDYSRLLL
                     IEYDTVTDAAHWTVAEPGPGLAVQSGARSLAITDGDTYLTFGYACAKQCTPQLHLREF
                     LPGGIPSGWNVLPAVNATFATDVAWSPAGYAVIAAGKVQGQWSTEFWLQGWVPGEEIP
                     AWEYGHLDQPGLQIALAVAVAKFGRIYAVGVAGQGDLGVPALAIIDP"
     gene            complement(2559..3536)
                     /locus_tag="VGB85_22540"
     CDS             complement(2559..3536)
                     /locus_tag="VGB85_22540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136885.1"
                     /translation="MVTTEGSSSTGGASGSTTGGTPESTGTSGTTSGSSGSGTGMVWD
                     LGSAPDLGDPTPPGCKGKVDFLFAISRAQNMPTAQEQLIASFAPFIASIEGLGAEFED
                     FDFHIMVVDGDRSWGMEYCEKNCGQDDFCDIMSSYPCAYEPTVCDETLGAGTIFNAGG
                     DTPNKNCGLAPGKRYLDTTTWALEETFTCIAQVGISGSDNLGNALGQAVTTQRETCNK
                     GFLRDDALLVVAHIMRGDVGSGVDGTPEEWANALLDAKGGDEDAIVMLGMNGFPDLET
                     CLEEDMRMCAMLTMFSHRVYATTEQASYAAAFQETVDLIDVACSEFVPG"
     gene            complement(3721..>5102)
                     /locus_tag="VGB85_22545"
     CDS             complement(3721..>5102)
                     /locus_tag="VGB85_22545"
                     /inference="COORDINATES: protein motif:HMM:NF044849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY0136886.1"
                     /translation="RPADLPVMTGAPPVVIVGAGPAGMFCAWGLARAGVRSVVLERGR
                     AVRARRHDLASLSQRGELDTESNYCFGEGGAGTFSDGKLYTRASKRGPVRLVLEALTG
                     YGAPPEILVDARPHIGTNRLPRVITAMREQLGTAGVEICFESRVDALLTGPSGQVRGV
                     RLRSGDEVEAAAVVVAPGHSARDVQRWLAAAGVAMSFKPFAVGVRIEHPQGLIDRLQY
                     AGLAGHPALGAASYRLVEQTPGGAVFSFCMCPGGYIVPAATDPGMQVVNGMSPKSRTG
                     GFANSGFVTEVGAAELARAGHDPTDPLAGVAYQAALEVLAYEAGGGNYVAPAQGLGDL
                     ARGRLSSALPPTSYHRGLTSARLDQLLGPLALPIQHALRALGERMPGFLGPEAVAVGL
                     ESRTSCPVRIERDSETLASPSTAGLYPCGEGAGFAGGIVSAALDGLRVAQAVAVQLGR
                     SADAVVAAI"
BASE COUNT          742 a         1894 c         1725 g          741 t
ORIGIN      
        1 gccggcatca ccgcgctgca ctactacgac cccgcgatcg gcccctggcg gatcagcaag
       61 cagcaggcgg cggtgttgtt cgcggtgatc gtcgcggtgg tcccgatgct cgcgatcggc
      121 ggctacctgc tgctgcgccg ccggctcccg gcgtggcacc gcttcgtcgt ccagtggctg
      181 ctcggcaagc gcctgtggct cggcatcggc ctccttatgc acctcggcat cgacgtgctg
      241 atgaacgtcg gcaccttcgt ccaggtgatg atcgccgtat acgtcgcgtg gctctccggc
      301 cccgagctcg acacgatctg gcacaccctg atgtcgcggc cccaggcgcc cggcaccggc
      361 gagcggccgc gctacaaaaa acgctggcag accctcctgc gcgcgccgat cgaccgcctg
      421 cgctttcgtg tgccgcggcc ggcctacgtc gtccatcaca accccggcga ggacagcgtc
      481 cgccgcgcgg cgctgctgcg cgtgtgggac ctcggcgacc gcctgagctt cgtcgcggac
      541 gacggcctcg cgccgcgacg gctgtgcgtc gaggtgcccg gcgtcgggct gcgctgcgac
      601 gccgacgccg gccgggcgct cgtcaccgtg ctgccgggct tctggtggat gtacccctgg
      661 tgcctgctgc cggggctgtc gcggctgtgc ggctggctcg tcgttcgcac gttccgctga
      721 ccgccgggct cggccgacct gacgcgctcg gtggcggcgc atcaggttgc atgctgccgt
      781 gtcgcgtgcg gctgggtgtg accggacctc cgccggcctc tccgtcgcgg gcaggttcta
      841 gatgcgcgat ttcgccaaac cgggtacggt gccacagatg cggccctcca ccctcgtgtt
      901 cctggtcgcg gtcgcggccg caggctgcgg cgacgcgcca gcgctggcca gcctgacctc
      961 ggcggcgacc gagaccccgc acggctcgac gagcacgggg accgacgagc ccgggtccac
     1021 gagcgagaca gggacacccg gcagcgacga gacgggcacc agcaccaccc cgtccggggc
     1081 gagtacgacg tcggacgcgg actccagcac gggccagccg gcgctaccga aactcctcga
     1141 ggcgacgctg accccggagg tgatcacggt cgagggttcg atcaccgcgc acgcgctggc
     1201 gcaggacgcg gacggcgtgc gcatgcaagt cgacggcggc gctccggtcg agctcgacga
     1261 gctggcaccc ggcgagttca cgggccagct cggggtcttc accgccctgt ccaacggcac
     1321 ccacgacgtc gtcttcaccg cctgggacga cagcaccgct agcgaacctc tctcccgcca
     1381 atacgaggcg aagctgaacc ccgcgggcgg cgagtcgtac tgggacgcct ccggcgtgct
     1441 cgggcagggc ttcggtcgcg gagtcgccat cgatccggcg accggcgcgg tctacgagtt
     1501 cttcgatgtt aaagaaggtg acggcgagcg ctgtcatgtc cgccgccgcg gcgcgcaggg
     1561 cgagtacttc gaggccgatc tgatcgcggt gctcccggag cagcagtgca cgggaaccgc
     1621 gatctcggtc gccgcggacg gcaccctgta cgtactcgcc gaggggcagc tcaacggcga
     1681 cgcaacgtgg tggctcggcc gcaaggacag ctgggacgcg ccgatgctga cgctcgcgtg
     1741 gggtgacccg ggtgaaaagg cgtacgcgat cgctgtgcac gccgagcggg ccgaggtggc
     1801 cgtctgcggc acgacgctga cggacggtac cgatttcgat gagctggacg ggagggttgc
     1861 aatcttccgc aaggacatgc cggggtacga gccggcgcca ttcgactaca cggcaaacca
     1921 gatcggcatg tcccacttct tcgacgagac gctgcgcgac tgcgcctacg acggggacac
     1981 cctggttctg gcgggcgagg caaatgggaa gcacccggac aacctcttcg tcgattacag
     2041 ccgcctcctg ctgatcgagt acgacaccgt caccgatgcc gcccactgga ccgtcgccga
     2101 accggggccg ggactcgccg tccagagcgg cgcccggtcg ctcgccatca ccgatggcga
     2161 cacctacctg accttcggct acgcctgcgc gaagcaatgc acgccgcagc tccacctgcg
     2221 cgagttcctc ccgggcggta ttccctcggg ctggaacgtg ttacccgccg tcaatgcgac
     2281 cttcgcaacc gacgtcgcct ggagcccggc cggctacgcc gtcatcgccg ccggcaaggt
     2341 ccaggggcag tggtccactg agttctggtt gcagggctgg gtgccggggg aggagatccc
     2401 tgcatgggag tacggccacc tcgaccagcc cggcttgcag atcgcgcttg cggtcgcggt
     2461 cgccaagttc ggccgcatct acgcggtcgg tgtcgccggg cagggcgacc tcggcgtgcc
     2521 cgccctggcg atcatcgacc cctgagatcg atcgcccgtc agccggggac gaactcggaa
     2581 caggcgacgt cgatcaggtc gaccgtctcc tgaaacgccg ccgcataact ggcctgttcc
     2641 gtggtcgcat aaacccggtg cgagaacatg gtgagcatgg cgcacatgcg catatcctcc
     2701 tcgaggcaag tctcaagatc ggggaaaccg ttcatgccga gcatgacgat cgcgtcctcg
     2761 tcgccaccct tggcgtcgag cagcgcattg gcccattcct ccggcgtgcc atccaccccg
     2821 gaccccacat ccccgcgcat gatgtgcgcc acgaccagca gcgcgtcgtc ccgcaggaac
     2881 cccttgttgc aagtctcgcg ctgggtcgtc acagcctggc ccagtgcgtt tccaaggttg
     2941 tcgctgccag atatgcccac ctgggcgatg caggtgaacg tctcctcgag cgcccaggtc
     3001 gtcgtgtcga ggtagcgctt gccgggcgcc aggccgcagt tcttgttggg ggtgtcgccg
     3061 ccagcgttga agatcgtgcc ggctccgagg gtctcgtcgc acacggtcgg ctcgtacgcg
     3121 cacgggtaac tcgacatgat gtcgcagaag tcgtcctggc cgcagttctt ctcgcagtat
     3181 tccatgcccc aactgcggtc cccgtcgacg accatgatgt gaaagtcgaa gtcctcgaac
     3241 tcggccccga ggccctcgat cgacgcgatg aacggcgcga agctggcgat caactgctcc
     3301 tgcgccgtcg gcatgttctg cgcacgcgag atcgcgaaca aaaagtcgac cttgcccttg
     3361 cagcccggcg gcgtcgggtc cccgaggtcc ggcgcgctcc ccaggtccca caccatgccg
     3421 gtgcccgacc cgctcgagcc cgacgtggtc cccgacgtgc ccgtgctctc gggcgtcccc
     3481 cctgtcgtcg agcccgaggc cccgcccgtg ctactcgagc cttcggtagt gaccacgggc
     3541 gccgtctggc caaacgacgt cgccccgacc ggacccggcg cacagccggc gaagctcagg
     3601 agtcccacca ggtcccatcc ggtgtgctgg cgccgcattt cgctcatcct accacgggac
     3661 gagcgaagtt gcgcatcaga cccttcggca gaccttcggc gatcggcggc cccccacccg
     3721 tcagatcgca gcgacgacgg cgtcggcgct gcgcccgagc tgcacggcga ccgcctgcgc
     3781 gacgcgcaga ccatcgagcg ccgccgacac gatgccgccc gcgaagccgg ccccctcgcc
     3841 gcagggatac agccccgcgg tgctcggtga cgccagcgtc tcgctgtctc gctcgatgcg
     3901 gaccgggcag ctcgtgcggc tctcgaggcc gaccgcgacc gcctcgggtc cgaggaaccc
     3961 cggcatgcgc tcgccgagag cccgcagcgc gtgctggatc ggcagcgcca gcgggccgag
     4021 cagctggtcg agccgcgcgg aggtcaggcc gcggtggtag ctcgtcggcg gcagcgcgga
     4081 ggacagccgc ccgcgcgcca ggtccccgag gccctgcgcc ggcgcaacat agttgccccc
     4141 gcccgcctcg tacgcgagga cctcgagcgc cgcctgatag gcgacccccg cgagcgggtc
     4201 cgtcgggtca tgacccgcgc gcgccagctc ggccgcgccg acctccgtca cgaaccccga
     4261 attggcgaag cctccggtcc gcgacttcgg gctcatgccg ttgaccacct gcatgccggg
     4321 gtcggtcgcc gccggcacga tgtagccgcc cgggcacatg cagaacgaga acaccgcgcc
     4381 gccgggcgtc tgttcgacga ggcggtacga cgcggcgccg agcgccgggt ggccggcgag
     4441 gccggcgtat tgcaggcggt cgatcagccc ctgcgggtgc tcgatgcgca cgccgacggc
     4501 gaacggcttg aagctcatcg ccacgccggc ggccgcgagc cagcgctgga cgtcgcgcgc
     4561 cgagtggccg ggcgcgacga ccacggcggc cgcctcgacc tcgtcgccgg atcgcagccg
     4621 gaccccgcgg acctgtcccg aaggaccggt gagcagcgcg tcgaccctcg actcgaagca
     4681 gatctcgacc ccggccgtgc ccagctgctc gcgcatcgcc gtgatcacgc gcgggagcct
     4741 gttggtgccg atgtgcggcc gcgcgtcgac gaggatctcc ggcggcgcgc catagcccgt
     4801 caacgcctcg agcaccagcc gcaccggccc gcgcttgctc gcccgcgtgt acagcttgcc
     4861 gtccgagaac gtgcccgccc cgccctcacc gaagcaatag ttgctctcgg tgtcgagctc
     4921 gccgcgctgg ctcaggctcg cgaggtcgtg ccgccgcgcc cgcacggccc gcccgcgctc
     4981 gagcaccacc gagcggaccc cggcgcgcgc gagcccccac gcgcagaaca tccccgccgg
     5041 cccggccccg acgatcacca ccggcggcgc cccggtcatc accggcaggt ccgcgggccg
     5101 tg
//
LOCUS       DASWNJ010001351         1664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_163847, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001351 DASWNJ010000000
VERSION     DASWNJ010001351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163847"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="VGB85_22550"
     CDS             complement(<1..931)
                     /locus_tag="VGB85_22550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136887.1"
                     /translation="MDNAQVMATDGSWFLFDDWQGMTRISLPSNRIKTDANLDRPSED
                     RPLVERPGGTVWYAYARDVDPVTLKRVKQDPEGHYSPDPLGGPEIVVEHLGAGVSIRG
                     ETLPLVASGDEIRLGGTPERRDTFIGGRYNIYPDRSLVVFGVAEAPVEASAGARAWRP
                     QVALVTRAPDGASRVAWCRPVARLQNSSPDAFRDAGKTWLADRDIVADIAYLVEVADD
                     GEVVGEARAPAVAGPWVLDGQVWWQSDDATLCSGARLGEAARSFTLAPEHAGPGRLLR
                     VPGRVLFLAWHRGTILDLAPAKRGKVELSRKHRV"
     gene            complement(1009..1527)
                     /locus_tag="VGB85_22555"
     CDS             complement(1009..1527)
                     /locus_tag="VGB85_22555"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY0136888.1"
                     /translation="MSQGSTDIDPLTGVWNRRRLLADLTEAFVQRRPATLVMFDLDNF
                     KHFNLHNGHLTGDEVLRAVPRVIASVLGAGDVVYRYAGQRFAATMPGSDMRAAAARAE
                     QIRAEIERTLAPPQLEHCGDRDCLGPVRPLTVSIAVVPPGGTVEETIALALETIHRAK
                     LEGRNRVCLAAS"
BASE COUNT          242 a          640 c          532 g          250 t
ORIGIN      
        1 cgaccctgtg tttgcgcgac agctcgacct tgccgcgctt cgccggcgcc agatcgagga
       61 tcgtgccgcg atgccaggcg aggaacaaca cccggccggg cacccgcagc agccgtccag
      121 gccccgcgtg ctccggcgcc agcgtgaacg atcgcgccgc ctcaccgagc cgcgccccgg
      181 agcacagcgt cgcgtcgtcg ctctgccacc acacctgccc gtcgagcacc cacggcccgg
      241 cgaccgcggg cgcccgcgcc tcgccgacga cctcgccgtc gtccgcgacc tcgaccagat
      301 acgcgatatc cgcgacgatg tcgcggtcgg cgagccaggt cttgccggcg tcacgaaacg
      361 cgtcgggcga gctgttttgc agccgcgcca ccgggcggca ccacgcgacg cggctggcac
      421 cgtcgggcgc gcgcgtcacc agtgcgacct gcggccgcca cgcgcgagcc cccgcgctcg
      481 cctcgaccgg cgcctcggcg acgccgaaca cgaccagcga acgatcggga taaatgttgt
      541 aacgcccccc gatgaatgtg tcgcgccgct ccggcgtgcc gccgagccgt atctcgtcgc
      601 cgctcgccac cagcgggagc gtctccccgc ggatcgacac gcccgcgccg agatgttcga
      661 ccacgatctc gggcccaccg agtgggtccg ggctgtagtg gccctccgga tcctgcttga
      721 ccctcttgag cgtcacggga tcgacgtcgc gcgcatacgc gtaccaaacc gtgccccccg
      781 gacgctcgac cagtggcctg tcctcgctcg gccgatcgag gttcgcgtcg gtcttgatcc
      841 ggttgctcgg caagctgatg cgggtcatcc cctgccagtc gtcgaacaaa aaccagctgc
      901 cgtcggtggc catgacctgt gcgttgtcca tgatcgtatg acttgagcta cacgaaaccg
      961 ccgcgaggct ccaccccgtc cgcccgggga ctgcgcgtca tcccggtgtc acgacgcggc
     1021 caggcatacg cgattgcggc cctccaactt ggcgcggtgg atcgtctcca gcgccagggc
     1081 gatcgtctcc tcgaccgtgc cacccggggg gacgaccgcg atcgacaccg tcagcgggcg
     1141 gaccgggccg aggcagtccc ggtcgccgca gtgctcgagc tgcggcggcg cgagcgtgcg
     1201 ctcgatctcc gccctgatct gctcggcgcg cgccgcagcg gcccgcatgt cgctccccgg
     1261 catcgtcgcc gcgaagcgct ggcccgcgta gcgatacacg acatcgcccg cccccagcac
     1321 cgacgcgatc acccgcggta cggcccgcag gacctcgtca cccgtgagat gtccattgtg
     1381 caaattgaaa tgcttgaagt tgtcgaggtc gaacatcacc agcgtcgccg gccggcgctg
     1441 tacaaacgcc tcggtgaggt cggcgagcag ccgccgccgg ttccacacgc cggtcagcgg
     1501 atcgatgtcg gtggatccct gggacatggt cgaggtctcg ccctgcggcg ccgacccaat
     1561 ctacccgaga tcatgatgca tggggcatgg gctcacttcc gtgcccgcgc acttctggta
     1621 gtgtccgccg catgcgcctg gccgtcctgc tgctcctcgc cgcc
//
LOCUS       DASWNJ010001352         2043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_201350, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001352 DASWNJ010000000
VERSION     DASWNJ010001352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201350"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..777
                     /locus_tag="VGB85_22560"
     CDS             97..777
                     /locus_tag="VGB85_22560"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0136889.1"
                     /translation="MRKPWSFETDRQMLRLLIGRRFGDAPEERVEEIREERRRHAAQV
                     VARMRIGAADVVADLGSGCGFMAGPVAGAARHLHCLDVSEDFAAVCARETADLANVTV
                     HVIEPGRLDKLAGVGVTKAFSHAVFMHLNLFDAVLYLREFRRVLPEGGLLLIDINDLD
                     RIDTAEDPAFNGHLDHYRRDRGVVFDLMQWHSAASFVRVARQNGFELLDQKPARSGAY
                     TELLLGRL"
     gene            complement(747..>2043)
                     /locus_tag="VGB85_22565"
     CDS             complement(747..>2043)
                     /locus_tag="VGB85_22565"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0136890.1"
                     /translation="GSAAWAQALRLLELVEIPAAPQRLREYPHLLSGGMRQRVMIALA
                     MACEPALLIADEPTTALDVTTQAQILGLVRELQAKHGTGVLFITHDFGVVAEIADRVA
                     VMRAGRVVETGTRDEVLRRPRDPYTRLLIAAVPSLTPRHRTLPPARPGQPQPAPVLRT
                     AGLGKVYSSGRFPRKRRVVPAAEGVTLEIRRGEVLGIVGESGSGKSTVARCVARLLAP
                     SSGEVWLGSDEIGRLPLRRLHPHRRRVQLVFQDPYRSLNPRRTVGASIVEGPMNYGIA
                     SSEAWRRAAELMGLVRLDADALGRYPHQFSGGQRQRICIARALAMEPELLIADEAVSA
                     LDVSVQAQVLRLLDQVRERLNLAMLFITHDLRVAAQVCDRVAVMHRGRIVEQGDVAQI
                     FAEPRHEYTKALFAAAPGRGYDFGGVADALQSRPSSSSV"
BASE COUNT          291 a          748 c          731 g          273 t
ORIGIN      
        1 acccctccgc cctacatcgc cgccgtggtc gcagccttga gtcaccacct ggacgactgg
       61 taagtaacgg gcttcgatcg ccggaggtgt cgacgcttgc gcaagccctg gagcttcgag
      121 accgaccggc agatgctgcg gctgctcatc ggccggcggt tcggcgacgc gccggaggag
      181 cgggtcgagg aaatccgcga ggagcggcgt cgccacgcgg cccaggtcgt ggcgcgaatg
      241 aggatcggtg ctgccgacgt ggtggccgac ctcggcagcg gctgcgggtt catggccggg
      301 ccggtagcag gggcggcacg ccacctccac tgcctcgacg tcagcgagga cttcgccgcc
      361 gtctgcgcgc gcgagacggc ggatctcgcc aacgtcacgg tgcacgtcat cgagcccggc
      421 cggctcgaca agctcgcggg cgtcggcgtc accaaggcgt tctcgcacgc ggtgttcatg
      481 cacctgaacc tgttcgacgc ggtgctctac ctgcgcgagt tccgccgcgt gctgcccgag
      541 ggcgggctgc tgctgatcga catcaacgac ctcgaccgga tcgacacggc cgaagacccg
      601 gccttcaacg gccacctcga ccactaccgg cgcgaccgcg gcgtggtgtt cgacctgatg
      661 cagtggcact cggcggcgtc gttcgtccgg gtcgcgcggc agaacggctt cgagctgctc
      721 gaccagaagc cggcgcggag cggcgcctac acggagctgc tgctaggccg gctctgaagc
      781 gcgtcagcga ccccgccaaa gtcgtagcca cggccgggtg ccgccgcgaa cagggccttg
      841 gtgtactcgt gccggggttc ggcgaagatc tgggccacgt cgccctgctc cacgatgcgc
      901 ccccggtgca tcaccgcgac ccggtcgcag acctgggccg cgacgcgcag gtcgtgggtg
      961 atgaacagca tcgcgaggtt cagccgctcg cggacctggt cgagcagccg gagcacctgg
     1021 gcctgcaccg agacgtccag cgccgagacc gcctcgtcgg cgatcagcag ctcgggctcc
     1081 atcgccagcg cccgggcgat gcagatccgt tgccgctgac cgccggagaa ctggtgcggg
     1141 tagcggccga gcgcgtcggc gtcgagccgg accaggccca tcagctcggc ggcccggcgc
     1201 caagcctccg aggaggcgat cccgtagttc ataggaccct cgacgatcga ggcgcccacg
     1261 gtgcggcgcg ggttgagcga tcggtagggg tcctggaaga cgagctggac ccggcggcgg
     1321 tgggggtgga gacgccgcag cgggagccgg ccgatctcgt ccgaccccag ccagacctcg
     1381 ccgctggagg gtgcgagcag ccgggccacg cagcgcgcca cggtcgactt gcccgagccc
     1441 gactcgccga cgatgcccag cacctcgccg cggcgaatct ccagcgtcac cccctccgcc
     1501 gcgggcacga cgcgccgctt tcgcgggaaa cgcccggagc tgtagacctt gccgagcccg
     1561 gccgtgcgca gcaccggggc cggctgcggc tgacccggtc gggccggcgg cagcgtccgg
     1621 tgccgcggcg tcaggctcgg caccgccgcg atcagcaggc gcgtgtacgg gtcccgcggc
     1681 cggcgcagca cctcgtcgcg ggtccccgtc tcgaccacac ggcccgcccg catcaccgcc
     1741 acccggtcgg cgatctccgc caccaccccg aagtcgtggg tgatgaacag gacgcccgtg
     1801 ccgtgcttcg cctggagctc gcggacgagg cccaggatct gcgcctgcgt ggtcacgtcg
     1861 agcgcggtcg tgggctcgtc ggcgatcagc agcgccggct cgcaggccat ggccagcgcg
     1921 atcatcacgc gctgccgcat gccccccgac agcaggtgcg ggtactcgcg cagccgttgc
     1981 ggtgccgccg ggatctccac cagttccagc aggcgcagcg cctgtgccca tgccgcggat
     2041 ccg
//
LOCUS       DASWNJ010001353         3367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_307512, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001353 DASWNJ010000000
VERSION     DASWNJ010001353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307512"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            466..2232
                     /locus_tag="VGB85_22570"
     CDS             466..2232
                     /locus_tag="VGB85_22570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136891.1"
                     /translation="MKLTAGPESESGDASVGPDTEDPQPTTGAETGDFPDETGGSSCV
                     EIREQAREVLIANCSGCHTGAMAIKFDYVDDLDALLKNGKVTGGDAASSVLYQQIEIG
                     AMPKGGPPLDDGDRAVVHDWIEQCTVPSVSDPLDAPSCVGQNKFISIDQMLGVMLDGI
                     NDFDDVEPDDQPFIRFFTLTHLWNAGYCDTQLDAHRHALAKLLNSLSNNGKIRVPKAI
                     DAERTIFRFDLRDYTWDKDLWELLVSENEFAVQYTQAKAVQLQALTGTKVPFQWADWF
                     VTDASEAPLYDQVLFERVLKVKPDVLNAAAPMTRFELEAKLGIDVEGDIADEIESNVD
                     LTSRAGFQFSGVSEQNRVIERHEFPGTFSRAYWISYDFLTEVETGNIFYHPLDFIADG
                     GEIIFNLANGLQAYLLVDSAGRRVDEADQDIVNNKEEGGDAIRNGRSCMGCHYQGMRL
                     IDDEIRVFVEGQVGVFDAETVEKVQHIYPSIEEFAATLTQDGDVFMSQLLKTGAPLLV
                     AGKEVISAAYHAFEDPYIDEGRAAAELGITAEALVPKIGQLPAALQMINGGVVSRQTM
                     RDQYAVAICNLKLGITKACKPG"
     gene            2252..3178
                     /locus_tag="VGB85_22575"
     CDS             2252..3178
                     /locus_tag="VGB85_22575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136892.1"
                     /translation="MPIRTSILHSWLSRAGAFLLALSLATPVVHAADPSRKPGASKKK
                     LGKRAEAEAAAKAKARADALKSALDEAQTQRDQALAGVRPLLSRGHHEDAALHLLDAA
                     LTYDDAVLHIEAAEQFLAAADRRHTAALARGRDAVTAARTLLAEVGPDPVADERVDVR
                     VVRVPHAQVEALLERCVAVERGLEARAKQLRRQTRGRQELAAGASLLAIGLTGFGVLA
                     GGTVYRAARREELAAIAGHESEYDLSALDAQGRRGDAMIGVGAVLGTVGVVLGASLLA
                     LGARDLRGRRAPELASLRVAPTLGGLVLSGRF"
BASE COUNT          543 a         1163 c         1157 g          504 t
ORIGIN      
        1 tgcacgggag cgaccgcctg tggatcagct tcctggcctc gcggatctac acggcgctca
       61 acgtgtggcg cgaggacatc acggacgtcg acgaggcggc gttccacgcc cgccacctcg
      121 cgtggttgca ggccgagcgc atgctgggtt gaggcgccag tttcgccggt gcgccgagtg
      181 atcggcccgg ggcggtacgg cgaatgggag gtgccacgcg aggccggccg cgatcacttc
      241 gcagcatcgt ctcgctggcg gatcgttctt cgctctcgca agtgatcgcg cggcccggca
      301 cgcggcactc ctggaggcga gacgccggcc gagtgggccg cctcgtccag gacacccatg
      361 cttcgccgac atccccagtc tgcctctatc catcgcctat ccgcccccta ttcgctgctc
      421 gcgagcctcc tcgtgtgcac ggccggatgc ccgctggccg aaccggtgaa gctcaccgcc
      481 ggccccgaga gcgagtccgg cgacgcgagt gtcgggcccg acaccgagga cccgcagccg
      541 acgaccggcg ccgagaccgg ggactttccg gacgagaccg gcggtagctc ctgcgtcgag
      601 atccgcgagc aggcccgcga ggtgctgatc gcgaactgta gcggctgtca caccggggcg
      661 atggcgatca agttcgacta tgtcgatgac ctcgacgcgc tgctcaagaa cggcaaggtc
      721 accggtggcg acgccgcgag ctcggtgctt tatcagcaga tcgagatcgg cgcgatgccc
      781 aagggcggcc cgccgctcga cgacggcgac cgggcggtgg tccacgactg gatcgagcag
      841 tgcacggtgc cctcggtgag cgacccgctc gatgcgccga gctgcgtcgg gcagaacaag
      901 ttcatctcga tcgatcagat gctcggcgtg atgctcgacg gcatcaacga cttcgatgac
      961 gtcgagccgg acgaccagcc gttcatccgc ttcttcacgc tgacgcacct gtggaacgcc
     1021 ggctactgcg acacgcagct cgacgcccac cgccacgcgc tcgccaagct gctcaacagc
     1081 ctctcgaaca acggcaagat ccgcgtgccg aaggcgatcg acgccgagcg gacgatcttt
     1141 cgcttcgacc tgcgcgacta cacgtgggac aaggacttgt gggagctgct ggtcagcgag
     1201 aacgagttcg cggtgcaata cacgcaggcc aaggccgtgc agctgcaggc gctgaccggc
     1261 accaaggtgc cgtttcagtg ggccgactgg ttcgtgaccg acgcctccga ggcgccgctc
     1321 tacgaccagg tgctgttcga gcgggtgctc aaggtcaagc cggacgtgct caacgcggcg
     1381 gcgccgatga cccgcttcga gctcgaggcg aagctcggca tcgacgtcga gggggacatc
     1441 gcggacgaga tcgagagcaa cgtcgatctc accagccgcg ccggcttcca gttctccggg
     1501 gtatcggagc agaaccgggt gatcgagcgt cacgagttcc cgggtacgtt tagccgggcg
     1561 tactggatca gctacgattt tctcaccgag gtcgagaccg gcaatatctt ctatcatccg
     1621 ctcgacttca tcgccgacgg cggcgagatc atcttcaacc tggcgaacgg actgcaggcg
     1681 tatctgctgg tcgacagcgc cggccggcgc gtcgacgagg ccgaccagga catcgtcaac
     1741 aacaaggaag agggcggcga cgcgatccgg aacggccgct cctgcatggg ctgccattat
     1801 cagggcatgc gcctgatcga cgacgagatc cgcgtcttcg tcgagggcca ggtcggggtg
     1861 ttcgacgccg agaccgtcga gaaggtccag cacatctacc cgtccatcga ggagttcgcg
     1921 gcgacgctga cgcaggacgg cgatgtgttc atgagccagc tgctcaagac cggcgcgccg
     1981 ctgctggtcg ccggcaagga ggtcatctcg gcggcctatc acgccttcga ggacccctat
     2041 atcgacgagg gccgcgccgc cgcggagctc gggatcaccg cggaggcgct cgtgccgaag
     2101 atcggccagc tcccggcggc gctccagatg atcaatggcg gcgttgtcag ccgccagacc
     2161 atgcgcgacc agtacgcggt cgcgatctgc aacctcaagc tcggcatcac caaggcctgc
     2221 aagcccggct gagcccagga ccatcaccat catgcccatc aggacctcca tcctccattc
     2281 atggctgtcg cgcgccggcg ccttcctgct cgcgctcagc ctcgccaccc cggtggtcca
     2341 cgccgccgat ccatcgcgca agccgggcgc gagcaagaag aagctcggca aacgcgccga
     2401 ggccgaggcc gcggcgaagg ccaaggctcg cgccgacgcg ctgaagtccg cgctcgacga
     2461 ggcccaaaca cagcgcgacc aggcgctggc gggcgttcgc ccgctgctca gccgcgggca
     2521 ccacgaggac gcggcgctgc acctgctcga cgcggccctg acgtacgacg acgcggtcct
     2581 ccacatcgag gcggcggagc agttcctcgc ggccgcggac cgccgtcaca ccgcggcgct
     2641 ggccaggggt cgagacgcgg tcaccgcggc gcgcacgctg ctcgcggagg tcggcccgga
     2701 cccggtggcg gacgagcgcg tcgacgtgcg cgtggtccgg gtcccgcacg ctcaggtcga
     2761 ggccctgctc gagcgctgcg tggcggtgga gcgcgggctc gaggcgcggg cgaagcagct
     2821 gcgccggcag acgcgtggtc gccaggagct ggccgcgggg gcctcgctgc tggcgatcgg
     2881 cctcaccggc ttcggcgtgc tcgcgggcgg cacggtctac cgcgcggcgc gaagggagga
     2941 gctggcggcg atcgccggtc acgaatccga gtacgacctc agtgccctcg atgcgcaggg
     3001 ccggcgcggc gacgcgatga tcggcgtcgg cgccgtgctc ggcacggtcg gggtggtgct
     3061 cggcgccagc ctgctcgccc tcggcgcccg cgacctccgc ggccgacgtg caccggagct
     3121 cgcgtcgcta cgtgtcgcgc cgaccctcgg cggactcgtg ctcagcggcc gcttctgaca
     3181 cgcgggctgg cgctggtgat cgctgcacgc tccgcgtgct ggacgccacg cgatcaggct
     3241 tcgtcttcag tgcatgcgca agaacgtggg aggtccgctg ttcgctcgac tcgccggccc
     3301 ggtgattttt ttcccggagg acatccttgt gctagtcgtc ctaatggagg catcgatgaa
     3361 accgacc
//
LOCUS       DASWNJ010001354         1594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_517170, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001354 DASWNJ010000000
VERSION     DASWNJ010001354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517170"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VGB85_22580"
     CDS             <1..222
                     /locus_tag="VGB85_22580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY0136893.1"
                     /translation="APMPGGVVELRVKAGETVAAGAPVVVLSAMKMETVVAAPHAGTV
                     QRIAVAVGDSVDAGDLLLVLTPAAASTAA"
     gene            complement(236..457)
                     /locus_tag="VGB85_22585"
     CDS             complement(236..457)
                     /locus_tag="VGB85_22585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136894.1"
                     /translation="MTADTAKVMGWGTIIAILVATALGIGLLLGLLGGALGWAPGRTG
                     GGVGVGVGLLAANLISRRRAALARANPPR"
     gene            586..>1594
                     /locus_tag="VGB85_22590"
     CDS             586..>1594
                     /locus_tag="VGB85_22590"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0136895.1"
                     /translation="MPRELRLALALATLAACTSNVPVNQEPAPEPVAQPEPSLPVPVP
                     PKPEGAPVNGFFDVGGMVRGEQTSTPPVIRRQYAKLPVKWKAKVGKTTFRTTMALTGG
                     SVVIGTHGATLDGKNEASDGVYVLDARTGKQTRLIRTPGAGDRDVGGIAIDGDTVYFT
                     TDNSQIVAATLAGKVLWTAKATGKVRPAPALADLDHDGTFDVVVGDESGELHALSGKT
                     GAPLWTVKTGESESGARGYIAAAAIADLDGDGNDDVIAAARDAILGAYRGKDGSVLWQ
                     HPGDSGVHASPTVADFDHDGKPEVLAAWSYGDVSVHDGKTGGLRWGTQLQRDDGGIEG
                     LF"
BASE COUNT          242 a          562 c          594 g          196 t
ORIGIN      
        1 gcgccgatgc ccggcggcgt ggtcgagctg cgcgtcaagg ccggcgagac ggtggccgcg
       61 ggcgcgccgg tcgtcgtgct ctcggcgatg aagatggaga ccgtggtcgc ggccccacac
      121 gcgggcacgg tgcagcggat cgccgtcgcg gtcggcgaca gcgtcgacgc gggcgacctg
      181 ctgctcgtgt tgaccccggc agccgcgtcc acggcggcct gaccgggtcc gcccgtcagc
      241 gtggcgggtt cgcccgcgcg agcgcggccc ggcgccgtga gatcaggttc gcggcgagca
      301 gcccgacgcc gacgccgacg ccaccgccgg tgcgcccggg cgcccacccg agcgcgccac
      361 cgaggagacc gaggagtaga ccgatgccca gggccgtggc gaccaggatc gcgatgatcg
      421 tgccccagcc catcaccttc gcagtgtctg ccgtcatatc gagccccacg gcgatcgagc
      481 gtgcgccggt tcgccggcga tcacaagccg tgccgctcgg cttcgcgcga cgggcagcgc
      541 gcggtgagac cgcaggccgc accgcgccgg gtgtaggctt cgcggatgcc tcgcgaactc
      601 cgcctcgccc tcgccctcgc cacgctcgcg gcctgcacga gcaacgtgcc cgtgaaccag
      661 gagcccgcgc cggagccggt ggcgcagccc gagccgtccc tgccggtccc cgtgccgccg
      721 aagccggagg gcgcgccggt caatggtttc ttcgatgtcg gcggaatggt ccgcggcgag
      781 cagacgagca cgccgccggt gatccgccgg caatatgcga agttgccggt gaagtggaag
      841 gcaaaggtcg gcaagaccac ctttcgcacg acgatggcgc tgaccggagg gtccgtcgtg
      901 atcggcaccc acggcgcgac gctcgacggc aagaacgagg cgtcggacgg cgtatatgtg
      961 ctcgacgcga ggactggcaa gcagacgcgg ctgatccgca cgcccggcgc cggggaccgc
     1021 gatgtcggtg gtatcgcgat cgacggtgat accgtgtact tcacgaccga caacagccag
     1081 atcgtcgcgg cgacgctcgc cggcaaggtg ctgtggacgg cgaaggcgac cggcaaggtc
     1141 cgccccgcgc ccgcgctcgc cgacctcgac cacgacggca ccttcgatgt cgtcgtcggc
     1201 gacgagtccg gcgagctgca cgccctgagc ggcaagaccg gcgcgccgct gtggacggtg
     1261 aagaccgggg agagcgagtc cggggcgcgt ggctacatcg cggccgcggc gatcgccgat
     1321 ctcgacgggg acggcaacga cgatgtcatc gccgccgcac gcgacgcgat cctcggcgca
     1381 tatcggggca aggacggcag cgtattgtgg caacatcccg gcgactccgg agtgcacgcg
     1441 tcgccgacgg tcgccgactt cgaccacgac ggcaagcccg aggtgctcgc ggcgtggtcg
     1501 tacggcgacg tcagcgttca tgacggcaag accggcgggc tgcgctgggg cacgcagctg
     1561 cagcgcgacg acggcggcat cgagggcctg ttcg
//
LOCUS       DASWNJ010001355         2319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_406680, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001355 DASWNJ010000000
VERSION     DASWNJ010001355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406680"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="VGB85_22595"
     CDS             complement(<1..969)
                     /locus_tag="VGB85_22595"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY0136896.1"
                     /translation="MNSSPNSPGAGSPNEPTADPTAPDAGPGGSSTAEPWATLEIRLR
                     TADTTGDGDWSAAGERLEALAAMIADDDRALGVETHDMSTLGQQTLHPELVVYTRPDA
                     LEGLGELATRLGEELRLDLALTATVRVDDDWRDTWKQFYAPIVFGSQDGSDSDGALLL
                     RPSWIDRRPGDPVVELVLDPGRAFGTGQHETTRLCVDEMIALQREGLAPGTVLDLGCG
                     SGILALAAARLFPGATTITAVDNDPEATETTRENADHNALTDRLEIRTGTARELDGRH
                     ALILANIRPSVLIPIAGDLAGLLAPGGSAVLSGSLGEEAEQVLAASV"
     gene            1239..1460
                     /locus_tag="VGB85_22600"
     CDS             1239..1460
                     /locus_tag="VGB85_22600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136897.1"
                     /translation="MAFTGVKVFSATKAWEREQISDRINEWLEHNPGVDIVDKVVTQS
                     SDSEFHCLTITLFYVRPTASNQQHAHAAV"
     gene            complement(1639..2202)
                     /locus_tag="VGB85_22605"
     CDS             complement(1639..2202)
                     /locus_tag="VGB85_22605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136898.1"
                     /translation="MVAAHGKFFLAGLASLALLACDLDEVEVKNDKGATYQTESESRG
                     TRVLTAPDGSKVTLQRDGTIIKPDGTKMQVKRDGTIVMPNGSRVRVFPDGSKSTLSIE
                     RTEFRSDGSTVVTAEDGSKTVIAADGSVSREFADGTRSRLTVVKPNATPTPVPPTTTT
                     TTTTTTTTPDPTPAPAPAPTPAPTKAG"
BASE COUNT          330 a          757 c          884 g          348 t
ORIGIN      
        1 gacggaggcg gcgagcacct gctcggcctc ctcgccgagg ctcccgctga gcaccgcgga
       61 gccgccgggc gcgagcaggc cggcgaggtc gccggcgatc gggatcagca cggagggccg
      121 gatgttggcg aggatcagcg cgtggcgacc gtcgagctcg cgcgcggtgc cggtgcggat
      181 ctcgaggcga tccgtcaagg cgttgtggtc ggcgttctcg cgggtggtct cggtcgcctc
      241 ggggtcgttg tcgacggcgg tgatcgtggt ggcgccgggg aacagccgcg ccgccgcgag
      301 cgcgaggatc cccgagccgc agccgaggtc gagcacggtg ccgggcgcga ggccctcgcg
      361 ctgcaaggcg atcatctcgt cgacgcacag gcgggtcgtc tcgtgctggc cagtgccgaa
      421 ggcgcggccg ggatcgagga ccagctcgac gacaggatcg ccgggtcggc gatcgatcca
      481 gctcggacgc agcagcagcg cgccgtcgct gtcggacccg tcctgcgagc cgaacacgat
      541 cggcgcgtag aactgcttcc aggtgtcgcg ccagtcgtcg tccacgcgca cggtcgcggt
      601 cagggcgagg tcgaggcgca gctcctcgcc gagccgcgtc gccagctcgc cgagcccctc
      661 gagcgcgtcg gggcgggtgt acacgaccag ctccgggtgc agggtctgct gcccgagggt
      721 gctcatgtcg tgggtctcga cgccgagcgc ccggtcgtcg tcggcgatca tcgcggccag
      781 ggcctcgagg cgctcacctg cggcgctcca gtcgccatcg ccggtcgtgt cggcggtgcg
      841 cagccggatc tccagcgtgg cccacggctc ggcggtcgac gatccgccgg ggccagcgtc
      901 cggggccgtc gggtccgcgg tgggttcgtt aggactgccg gcgcccggcg aattcggcga
      961 ggagttcatg cggcgcagtg tagcggcgcc gctggcccct gcggaatcgg gtcgatgtgt
     1021 agagaatgtc gtttgaatta caaaaattgc acaaggtatt cgtaagaaca gtacccggga
     1081 tcctgacatg acccgccgcc ggcccggcca tcgcccgcca gggggccaca ggggccagca
     1141 gatttgacct ttataaattc gcgcaggggt gtcatagagg ggatgtgcgg gcccatgcaa
     1201 agacgacaac cggcccggcg aacggggaga ggaacatggt ggcatttacc ggagtcaagg
     1261 tgttctcggc gaccaaggcc tgggagcgcg agcagatcag cgatcgcatc aacgagtggc
     1321 tggagcacaa ccctggcgtc gatatcgtcg acaaggttgt cacgcagagc tccgacagcg
     1381 agtttcactg cctgacgatc acgctgttct acgtgcgacc cacggccagc aatcagcaac
     1441 acgcccacgc cgcagtctag gctgccggcg cgggagatcg tccctaccac attagtctgc
     1501 gaccgcgagc cgccgctggc ccccccggcg gcggctcgcg gacgtcgtgg gggctcaggg
     1561 ccggagggcc ggggagcagc tcagggccgg agggccgggg agcagctcag gcccggaggg
     1621 ccggggagca gcctcgggtc agccggcctt cgttggggcg ggcgtcggcg cgggggccgg
     1681 ggccggcgtg gggtccgggg tcgtcgtggt ggtggtcgtg gtggtggtcg tcgtcggcgg
     1741 cacgggggtc ggcgtcgcgt tgggcttcac gacggtgagg cgcgagcggg tgccgtcggc
     1801 gaactcgcgg gacaccgagc cgtcggcggc gatgacggtc ttgctgccgt cctcggcggt
     1861 gacgacggtc gagccatcgc tgcggaactc ggtgcgctcg atcgatagcg tcgacttgga
     1921 accgtcgggg aacacccgca cgcgcgagcc gttgggcatg acgatcgtgc cgtcgcgctt
     1981 gacctgcatc ttggtgccgt caggtttgat gatcgtgccg tcgcgctgca gcgtcacctt
     2041 ggagccgtcc ggggcggtca gcacccgcgt gccgcgtgac tcggactcgg tctggtaggt
     2101 cgcgcccttg tcgttcttga cctcgacctc gtcgagatcg caggccagca gcgccagaga
     2161 cgccagaccc gccagaaaaa acttcccgtg tgccgccacc atcggaccac gagcctatca
     2221 cggcgcgggc cgtcgtcggc gcgtcatggc ttgcacggtg cagacctccc aggcggaccg
     2281 gcccggggcc cccgcgcggg gccgcgcggt cgatcccgc
//
LOCUS       DASWNJ010001356         2630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_201400, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001356 DASWNJ010000000
VERSION     DASWNJ010001356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201400"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2630)
                     /locus_tag="VGB85_22610"
     CDS             complement(<1..>2630)
                     /locus_tag="VGB85_22610"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0136899.1"
                     /translation="LQRSTPYLALSQALRSIVRRRLADTAEALARWKTVWTQAAGPNG
                     QILVGLMPELVHILGVPAPLAEVGPVEAKNRFLLTVQYFLRATASPEHPLVLFIDDLQ
                     WADPASMTLLLQIVTDPAAAHILVVGAYRDNEVDAEHPLHALARATREAGRGVQTISL
                     APLGIDALIGMVADMLGQPAEAVGPLAALIKSKTDGSPFFVEQYVQALHAQRLLVRDR
                     ETGAWQWDAGQLERAGVTDNVAALLTEKLGQLSPTARRVLALGACAGASFEAALVGEA
                     GELTAPALQVAVEELKDHGLVLAATDAGPDVCEFVHDRVQQAAYALLADEPEQAHAHD
                     ALARTLDRRERGDAELFAMLYHYLRALTRLRDADDRQRVAGHCLRGGQRAKDGSAYVE
                     AVKFLHAGRELLGESGWDAHFDLTFEIHLALAEAEWLAGLPEIGEPLVETCFARAATR
                     QARGRVTIVWITLLCLSGRYPDATARGLATLEEFGWRFPQRSEEIYAFMGAQVGAIVP
                     RLMSATSEQLAAWPRCTDPEAIMAGALLARVAVAVVYARPELFPTICFAMVEHTLRHG
                     VSWMTAVGGGAGAMMSVLFLQELGLARRLVEFGRLHLRDAVGKTAYTINAMSLARQYF
                     DPLVVVSEYWESGEEIGAREGDISFSEYCAVMPALTRLLGPGSLLRMPALRADYTDFS
                     SRETAMSAAGVRAALTQASPREALELARGWTRAASPTPYLMHWANACTAFTALHLDED
                     ELALEHALIAEPFWPASICGPNLIVLCFALCLGAARYPDRVAGKTAEVAAQLARLGKW
                     AEFTPESFLHVKLLVDAWQARNDGRHADAERLFEAAIADARAHGFLNDEALGLRMAGE
                     YFCERGLARAARGYLEDACET"
BASE COUNT          383 a          940 c          966 g          341 t
ORIGIN      
        1 gtctcgcacg cgtcctcgag gtacccccgg gccgcgcggg ccagcccgcg ctcgcagaag
       61 tactcgccgg ccatgcgcag gccgagcgcc tcgtcgttga gaaagccgtg cgcgcgcgcg
      121 tcggcgatcg cggcctcgaa caggcgctcg gcgtcggcgt ggcggccgtc gttgcgggcc
      181 tgccaggcgt cgacgagcag cttgacgtgc aaaaagctct cgggggtaaa ctcggcccat
      241 ttgccgagcc gggccagctg cgcggcgacc tcggcggtct tcccggcgac cctgtcaggg
      301 tagcgggcgg cgccgaggca cagcgcgaag cacagcacga tcaggttcgg gccgcagatc
      361 gacgccggcc agaatggttc ggcgatcaac gcgtgctcga gggccagctc gtcctcgtcg
      421 aggtgcagcg ccgtgaacgc ggtgcacgca ttggcccaat gcatcaagta cggggtcggc
      481 gaggcggcgc gggtccagcc gcgggccagc tcgagcgcct cgcgcgggct ggcttgcgtc
      541 aacgcggcgc gcacgcccgc ggccgacatc gccgtctcgc gcgacgagaa atcggtgtag
      601 tcggcccgca gcgccggcat gcgcagcagc gagcccgggc cgagcaggcg cgtcagggcc
      661 ggcatcacgg cgcagtactc actaaacgag atgtcaccct cgcgcgcgcc gatctcctcg
      721 ccgctctccc agtattcgct gacgacgacg agcgggtcga agtattgccg ggcgaggctc
      781 atcgcattga tcgtataggc ggtcttgccg acggcgtcgc gcaggtggag gcgaccgaac
      841 tcgacgagtc ggcgggccag gccgagctct tgcaggaaca gcaccgacat catggccccc
      901 gcgccgccgc cgacggccgt catccacgag acgccgtggc gcagggtgtg ctcgaccatg
      961 gcgaagcaga tcgtcgggaa cagctcgggc ctcgcgtaca cgacggcgac ggcgacgcgg
     1021 gcgagcagcg ccccggccat gatcgcctcg gggtcggtgc agcgcggcca cgcggccagc
     1081 tgctccgagg tcgcggacat cagccgcggc acgatcgcgc cgacctgcgc gcccatgaac
     1141 gcgtagatct cctcgctgcg ctgcgggaag cgccagccga attcctcgag cgtcgccagg
     1201 ccgcgggcgg tcgcgtcggg gtagcgcccc gacaggcaca gcagcgtgat ccacacgatc
     1261 gtcacccggc cgcgcgcctg ccgggtcgcc gcgcgcgcga agcaggtctc gacgagtggc
     1321 tcgccgatct cggggaggcc cgcgagccac tcggcctcgg cgagcgcgag gtggatctcg
     1381 aaggtgaggt cgaagtgcgc gtcccagccc gattcgccga gcagctcgcg gccggcgtgc
     1441 aggaacttca ccgcctcgac gtaggccgaa ccatccttgg cccgctgccc accacgcagg
     1501 cagtgcccgg cgacccgctg gcgatcgtcg gcgtcgcgca gccgcgtcag cgcgcgcagg
     1561 tagtgataca gcatcgcgaa cagctcggcg tcgccgcgct cgcggcggtc gagcgtgcgc
     1621 gcgagggcgt cgtgcgcgtg ggcctgctcg ggctcgtcgg cgagcaacgc gtacgcggcc
     1681 tgctgcacgc ggtcgtgcac gaactcgcag acgtcgggcc ccgcgtcggt cgccgcgagc
     1741 accagcccgt gatccttgag ctcctcgacg gcgacctgca gcgccggcgc cgtcagctcg
     1801 ccggcctcgc cgacgagcgc ggcctcgaag ctggcgccgg cgcaggcgcc gagggcgagc
     1861 acacgccggg cggtcggcga cagctggccg agcttctcgg tcagcagggc ggcgacgtta
     1921 tcggtgacgc cggcgcgctc gagctgtccg gcgtcccact gccaggcgcc ggtctcccgg
     1981 tcgcgcacga gcagccgctg cgcgtgcagg gcctgcacat attgctcgac gaagaacggg
     2041 ctgccgtcgg tcttggactt gatcagcgcc gcgagcgggc cgacggcctc cgccggctgg
     2101 ccgagcatat cggcgaccat cccgatcaat gcatcgatcc cgagcggcgc gagcgagatc
     2161 gtctgcacgc cgcgcccggc ctcgcgggtc gcccgggcca gcgcgtgcag cgggtgctcc
     2221 gcgtcgacct cgttgtcgcg atatgcaccg accacgagga tatgcgcggc cgccgggtcg
     2281 gtgacgatct gcagcagcag ggtcatcgac gccgggtcgg cccattgcag gtcgtcgatg
     2341 aacagcacca gcgggtgctc ggggctcgcc gtggcgcgga ggaagtactg caccgtcagc
     2401 aagaagcggt tcttggcctc gaccgggccg acctcagcca gcggcgcggg cacaccgagg
     2461 atatggacca gctcgggcat caggccgacg aggatctggc cgttggggcc cgccgcctgc
     2521 gtccacaccg tcttccagcg ggcgagcgcc tcggcggtgt cggccagccg gcggcgcacg
     2581 atcgatcgca gcgcctgcga cagcgcgagg tacggggtgc tgcgctgcag
//
LOCUS       DASWNJ010001357         5731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_660982, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001357 DASWNJ010000000
VERSION     DASWNJ010001357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660982"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1305)
                     /locus_tag="VGB85_22615"
     CDS             complement(<1..1305)
                     /locus_tag="VGB85_22615"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY0136900.1"
                     /translation="MWRAARSGSRRQLVTLRCYHDRLVLVFVLAADLAAALAADLGEP
                     TSKERIVNLPALGRPGDRRAYPRVTCTLTCAVPGLGEGRVLNVSDGGAFIALPAAQVH
                     PPRHIQVRLGLTDPPIELPAEVVRVTTLEPHGLGLGLQFRSVNDTAGEHIHRFVLARL
                     LAEIAEIMEGDPRPVDPRNVQTISSVESVAAAMRQMIEAEGPVPGTLFQRDVGELVDI
                     QLLAADHRTATVSLRDPNARRPVAGDHIHLTLTRGSFNAHAHTQVLSRNGERVSLDLP
                     ASLTVFELRRAPRRAPAPEEMFISIPVPYPPATKLRCEVLDISSTGLAFKLAPNAPYF
                     LPGTPLREVAISGLGGDDERRKSAQVMHITPVDDEHGKLSYLRVGIDFGITDENFAKG
                     ARPRAGGEKRSVSFVEKMGMLIGRLVPKRGPQTSAIAASAGVE"
     gene            1734..2141
                     /locus_tag="VGB85_22620"
     CDS             1734..2141
                     /locus_tag="VGB85_22620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136901.1"
                     /translation="MLRIFAVLALSSTVLSAVACKKDDATNKPGGCEAGFVMTQGNVC
                     VMQNIVDYNQCVESAAARESINVGLTKEILGRLQLVWVEPGIGPTPEARAKAAKFIQL
                     NPGSCRELEVVWGCYQVANKVQEDASAAPICAK"
     gene            complement(2234..3229)
                     /locus_tag="VGB85_22625"
     CDS             complement(2234..3229)
                     /locus_tag="VGB85_22625"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEY0136902.1"
                     /translation="MIDLRMWARALRVIPRVSKPEWDRLDIVSRWLIATRAAVFIMTA
                     VSAGVGGLLAVRDGVFSGPRFVACLLGLVFAHATNNLLNDLTDHLKGVDRDNYFRAQY
                     GPQPLEHGLLTIRQLLRYIAFSGAIALVAGIYLVAVTGGVTLSLFAAGIFFVLFYTWP
                     LKYYGLGEPAVLLVWGPLMVGGTYFVVSNGHYDPSVTWISLAYAIGPTAVLFGKHTDK
                     LEADRAKGIRTLPVLLGEPAARLGVVGMLIVQQLLTLGLVARGALGWPMLLVLFALPA
                     LRETIAVFLRPRPTTRPEAFPAHIWPIYLVAYAFRYNRVFGLLFLGGLLIDLWLS"
     gene            3308..4138
                     /locus_tag="VGB85_22630"
     CDS             3308..4138
                     /locus_tag="VGB85_22630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136903.1"
                     /translation="MTILKRSLRIGLPVLLLAGGCKGDDETATESQSTSDTAAPSTGG
                     SSPTEAGETDTGGGGDGGGDGTGTTGEQPTTGDDPTAPTTGEPVACDTPEGCTAMGGG
                     EVASTTLPFFRGEVCISDKLRTGDPLALSVTTCVHPCLKTDGYKFKWVYRCTEGVCEA
                     GLVFYHPNVTGSMCPADVFGAFDPAGCKFTLPAALAIKLPDTMGDASLLMPFLTNDDA
                     AAIDGGETENAVIWGRIDGHVQASERRLPINFAAENPAPPATCSAGEPGCTCTKIGVP
                     "
     gene            complement(4142..4933)
                     /locus_tag="VGB85_22635"
     CDS             complement(4142..4933)
                     /locus_tag="VGB85_22635"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0136904.1"
                     /translation="MTTPLHKAPIIDLTGKVAIITGSSRGIGRSIAETMARAGARVVV
                     SSRKRGPCEEVAEGIRGAGGEAIVIPCNISDKAQVENLVDETLATWGRVDSLVCNAAS
                     NPYFGPLTQVTDEIFTKIMHNNVLANLWLARRVVPGMTERRDGSIIVVSSIGGLRGDA
                     WLGTYNISKAADMQMVRNLAVELGPANIRVNGIAPGLIKTDFARALWDNPEILERIKR
                     STPLGRIGEPDEIGGFAAFLASDRASFLTGQVVVIDGGLTTTGAW"
     gene            complement(5308..>5731)
                     /locus_tag="VGB85_22640"
     CDS             complement(5308..>5731)
                     /locus_tag="VGB85_22640"
                     /inference="COORDINATES: protein motif:HMM:NF015376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HEY0136905.1"
                     /translation="PKVRVGEIAKRSGGRVAPHASHRSGRDVDLGYIFNGGDDGETRW
                     QRMSERNFDVEKNWFLIQELLKSGKVETIYISKKLQKLLYKEAAKTLSEPELAALFEY
                     PRTDASPHAVLQHWRGHGDHMHVRFQCEPGNRRCRSRH"
BASE COUNT          903 a         1771 c         2205 g          852 t
ORIGIN      
        1 ctcgacgccc gcgctggcgg cgatcgccga ggtctgcggc ccgcgcttgg ggacgaggcg
       61 gccgatcagc atgcccatct tctcgacgaa gctgaccgac cgcttctcgc cgccggcgcg
      121 cgggcgggcg cccttggcga agttctcgtc ggtgatgccg aagtcgatgc cgacccgcag
      181 gtacgagagc ttgccgtgct cgtcgtcgac gggggtgatg tgcatgacct gggccgactt
      241 gcggcgctcg tcgtcgccgc cgaggcccga aatggcgacc tcgcgcaacg gggtgccggg
      301 caggaagtag ggggcgttcg gggcgagctt gaaggcgagg ccggtgctcg agatgtcgag
      361 gacctcgcag cgcagcttgg tcgccggcgg gtagggcacc gggatcgaga tgaacatctc
      421 ctcgggcgcg ggggcgcgac gaggggcgcg gcgcagctcg aagacggtga ggctggcggg
      481 caagtcgagg ctgacgcgct cgccgttgcg gctgagcacc tgggtgtgcg cgtgggcgtt
      541 gaacgagccg cgggtgaggg tgaggtggat gtggtcgccg gcgacgggcc ggcgcgcgtt
      601 ggggtcgcgc agcgagacgg tggcggtgcg gtgatcggcg gcgagcagct ggatgtcgac
      661 gagctcgccg acgtcgcgct ggaacagggt gccgggcacg gggccctcgg cctcgatcat
      721 ctggcgcatg gcggcggcga cggactcgac cgagctgatc gtctggacgt ttcgcgggtc
      781 gaccgggcgc gggtcgccct ccatgatctc cgcgatctcg gcgagcaggc gggcgagcac
      841 gaagcggtgg atgtgctcgc cggcggtgtc gttgacgctg cgaaattgta agccgaggcc
      901 gaggccgtgc ggctcgaggg tcgtgacgcg gacgacctcc gcggggagct cgatcggcgg
      961 gtcggtgagc ccgagtcgca cctggatgtg cctgggcggg tggacctggg cggcgggcaa
     1021 ggcgataaag gctcccccgt cggagacgtt gaggacgcgc ccctctccga gcccggggac
     1081 ggcgcaggtg agcgtacaag tcacacgagg ataggcgcgg cggtcaccgg gcctcccgag
     1141 agcaggaaga ttcacgatcc tctccttgga tgttggttcg ccgaggtcgg cagcgagtgc
     1201 ggcagcgagg tcggcagcga ggacaaaaac gaggacaaga cggtcatggt aacaccgcaa
     1261 tgtgaccagt tgccggcgcg atcccgatcg ggcggcgcgc cacatccgcc cggggagccg
     1321 ccacgggcga cctggggccg gatgtctcaa gggacaggag catgggcagg catggaatat
     1381 ggcggtgctg tggcagtgca ccggcaatgg tggttcgcga cgtcagtggc gatgtaagcc
     1441 aaggtcgtcc ctgccgggac agggccgggg gacccccccg cgccggtacg cgggcccccg
     1501 gcccaggggc tctcggtttt tcttaaaact tcgcgtcacg ggaggtcgag cgcgaagttt
     1561 ctcgaagtgg ttggatcgtg gcgtgtcggt cgggcccgga tcacaggctg gctcacgtaa
     1621 tggacagcac ggacgacgtt tgtcacgggg gtgccgcggg attggcgatt ttcgtgctct
     1681 ataggcgcgg ctgtggcata agatgggccg caacacgcca gaggctcccc cccatgcttc
     1741 gtatttttgc cgtcctcgcc ctctcgtcca ccgtcctcag cgccgtcgcc tgcaagaagg
     1801 acgacgccac gaacaagccg ggcggctgtg aagccggctt cgtgatgacg cagggcaatg
     1861 tctgcgtcat gcagaacatc gtcgattaca atcagtgcgt ggagagcgct gccgcgcgcg
     1921 agagcatcaa cgtcggcctc accaaggaga tcctcggtcg cctgcagctg gtgtgggtcg
     1981 agccgggcat cggtcccacc ccggaggcgc gggccaaggc cgccaagttc atccagctca
     2041 accccggctc gtgccgcgag ctcgaggtcg tgtggggctg ctaccaggtc gccaacaagg
     2101 tccaggaaga cgccagcgcc gccccgatct gcgcgaagtg agccgcgagc tcgccagggc
     2161 tcgctcgatc ctcgcttgat cagagatcat gacgacccgg ccgcggtgcg gcgcgggtcg
     2221 ttttgttttg tggtcaggag agccagagat cgatgagcag gccgccgagg aacagcaggc
     2281 cgaacacgcg attgtaacgg aacgcgtagg cgacgaggta gatcggccag atgtgggccg
     2341 ggaaggcctc ggggcgggtc gtggggcgcg ggcgcaggaa cacggcgatc gtctcgcgca
     2401 gggccggcag ggcgaacagg accagcagca tcggccagcc gagcgcgccg cgggcgacga
     2461 ggccgagggt cagcagctgc tggacgatca acatgccgac gacgccgagg cgcgcggccg
     2521 gctcgccgag cagcaccggc aaggtgcgga tgcctttggc gcggtcggcc tcgagcttgt
     2581 cggtgtgctt gccgaacagc acggcggtcg gaccgatcgc gtaggcgagc gagatccagg
     2641 tgaccgaggg gtcgtagtgg ccgttgctga cgacgaagta ggtgccaccg accatcagcg
     2701 ggccccacac cagcaggaca gcgggctcgc cgaggccgta gtacttgagc ggccaggtgt
     2761 agaacaggac gaagaagatg ccggcggcga acaggctgag cgtgacgccg ccggtgacgg
     2821 cgacgaggta gatcccggcc acgagggcga tcgcgccgga gaaggcgatg tagcgcagga
     2881 gctggcggat cgtcagcagg ccgtgctcga gcggctgcgg gccgtactgg gcgcgaaagt
     2941 aattgtcgcg gtcgacgccc ttgaggtggt cggtcaggtc gttgaggagg ttgttggtcg
     3001 cgtgggcgaa cacgaggccg agcaggcagg cgacaaagcg cggtccggag aacacgccgt
     3061 cccgcacggc gagcagtcca ccgacgccgg ccgacacggc ggtcatgatg aagacggccg
     3121 cgcgcgtggc gatcagccag cgactgacga tgtcgaggcg gtcccactcg ggcttggaga
     3181 cccgtgggat cactcgaagg gcgcgtgccc acatgcggag gtcgatcatg cggggacgct
     3241 cgcacgcggg gtcgcgtggg gcaactgcgc gggtgtcggc gcccggggcc gcggtacact
     3301 ggtgcggatg acgatcttga agcgttccct gcgcattggc ttgccggtgc tgctcctcgc
     3361 gggcggctgc aaaggtgatg acgagacggc gacggagtcg cagtcgacga gcgacacggc
     3421 ggcaccatcg accggcggca gcagtccgac cgaggccggc gagacggaca ccgggggcgg
     3481 cggcgacggc gggggcgacg ggaccggcac gaccggcgaa cagccgacga ccggcgatga
     3541 cccgaccgcg ccgacgaccg gcgagccggt ggcctgcgac acccccgagg gctgcacggc
     3601 gatgggcggc ggcgaggtgg cgagcacgac gctgccgttc tttcgcggcg aggtgtgtat
     3661 cagcgacaag ctgcgcacgg gtgacccgct ggcgctgtcg gtgacgacct gcgtgcaccc
     3721 gtgcctcaag accgacggct acaagttcaa gtgggtgtac cgctgcacgg aaggggtgtg
     3781 cgaggccggg ctggtgttct atcacccgaa cgtgaccggc agcatgtgcc cggcggacgt
     3841 gttcggcgcg ttcgacccgg ccggttgcaa gttcacgctg ccggcggcgc tggcgatcaa
     3901 gctgcccgac acgatgggcg acgcgtcgct gctgatgccg ttcctgacca acgacgacgc
     3961 ggcggcgatc gacggcggcg aaaccgagaa cgcggtgata tggggccgca tcgacggtca
     4021 cgtgcaggcg agcgagcgcc ggctgccgat caacttcgcg gccgagaacc ccgcgccgcc
     4081 ggcgacgtgc agcgccggcg agccgggttg tacgtgcacg aagatcggcg tgccgtagcg
     4141 atcaccacgc gccggtggtc gtcaggccgc cgtcgatgac gacgacctgg ccagtgagga
     4201 agctggcccg gtccgaggcg aggaaggccg cgaacccgcc gatctcgtcg ggctccccga
     4261 tccgcccgag cggggtgctg cgcttgatcc gctcgaggat ctcggggttg tcccacagcg
     4321 ccctcgcaaa gtcggtcttg atcaggcccg gcgcgatgcc gttgacgcgg atgttggcag
     4381 gcccgagctc gaccgcgagg ttgcgtacca tctgcatgtc ggcggccttc gagatgttgt
     4441 acgtgccgag ccaggcgtcc ccgcgcagac cgccgatgct cgagacgacg atgatgctgc
     4501 cgtcacgccg ctcggtcatc ccgggcacga cgcgacgcgc cagccacagg ttcgccagca
     4561 cgttgttgtg catgatcttg gtgaagatct cgtcggtcac ctgggtcagc ggaccgaagt
     4621 aagggttgct cgcggcgttg cacacgaggc tgtcgacgcg accccaggtg gcgagggtct
     4681 cgtcgaccaa attctcgacc tgcgccttgt ccgagatgtt gcacgggatg acgatcgcct
     4741 cgccgccagc gccgcgaatg ccctccgcga cctcctcgca cgggccacgc ttgcggctgg
     4801 agacgacgac ccgcgccccg gcccgcgcca tggtctccgc gatcgaccgg ccgatgccgc
     4861 ggctcgaccc ggtgatgatg gcgaccttgc cggtcaggtc gatgatcgga gccttgtgaa
     4921 gaggtgtggt catacggggg cgctttcgcg cgaacgatgc ctgccattcg cgtgctgggc
     4981 aagagtatcc gggtggatag gagagcgtgg gctcgggggt ctgcggggcc ccctccacct
     5041 gcgtccctgg cattcggcct gccccccggg cgggcatcgt cggcgccccg ctttcgacca
     5101 cgcagggatc gtttgctcgg cgagctccgg gaagctttgg cgtcgtcggg ggccgacggg
     5161 actcgccagg tgcgagcagg gccgcgttgg ccggactcgc caggcgcgag cagggtcgcg
     5221 ttgcccggtc acgcaggcgc gagcagggcc cttaaacagc caagccccgc gttgccggtc
     5281 ggcaaggcgg ggcgagcggg gagaagctca gtggcgggag cggcagcggc ggttgccggg
     5341 ctcgcactgg aagcggacgt gcatgtggtc gccgtggccg cgccagtgct ggaggacggc
     5401 gtgcgggctg gcgtcggtgc gcgggtactc gaagagggcg gcgagctcgg gctccgagag
     5461 ggtcttggcc gcctccttat agaggagctt ctggagcttc ttgctgatgt agatcgtctc
     5521 gaccttgccg gacttgagca gctcctggat gaggaaccag ttcttctcga cgtcgaagtt
     5581 gcgctcgctc atccgctgcc agcgggtctc gccgtcgtcg ccaccgttga agatgtagcc
     5641 gaggtcgaca tcgcggccgg agcggtggga ggcgtgcggg gcgacgcggc cgccgctgcg
     5701 cttggcgatc tcgccgacgc ggaccttggg g
//
LOCUS       DASWNJ010001358         1551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_621297, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001358 DASWNJ010000000
VERSION     DASWNJ010001358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621297"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VGB85_22645"
     CDS             complement(<1..675)
                     /locus_tag="VGB85_22645"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEY0136906.1"
                     /translation="MTAARPRRRFVTFLSRSLTVIGGITTALFVLLLIFVAFTCAVGG
                     GKVPTKTIIEIDFERSFVEYGSTDPVSRIFGGGDANVAELVAALERAGEDDRVVGLVG
                     HIGAGGYGMAMTQEMRDAVQAFRAKGKFAVAYSESFGEFGPGNQGYYLAAAFDEIWLQ
                     PSGDVGLNGIAAQTMFLRGTFDKLDVDVRMDHRHEYKSAKNTYTERDYTPAHREALAA
                     VLTSMHT"
     gene            complement(672..1364)
                     /locus_tag="VGB85_22650"
     CDS             complement(672..1364)
                     /locus_tag="VGB85_22650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0136907.1"
                     /translation="MVDDFALLEAWRGGDLQAGSQLFDRHFESIHRFLRNKVGDDTDE
                     VMQRTLLACVETRDRFRGASSFRTYLFGVARLELLAHYRRRRKMDNQVDLESRSFYDL
                     DPSPSTVVAKHMEQKILHEALRRLPIVLLIALELHFWENMSATEVATVLEIPSGTVKS
                     RLRRAKEMLRDTMSGLQLSKPALESTLGDLEGWAKSLGETLREGAAVSRKPAIDSLDS
                     LDGLDAHDETTP"
BASE COUNT          228 a          469 c          617 g          237 t
ORIGIN      
        1 cgtgtgcatg ctggtcagca cggccgcgag cgcctcgcgg tgcgcggggg tgtagtcgcg
       61 ctcggtgtag gtgttcttgg cgctcttgta ctcgtggcga tggtccatgc ggacgtcgac
      121 gtcgagcttg tcgaaggtgc cgcgcaggaa catggtctgc gcggcgatgc cgttgaggcc
      181 gacatcaccg gagggctgca gccagatctc gtcgaaggcg gcggcgaggt agtagccctg
      241 gttgccgggg ccgaactcgc cgaagctctc ggagtaggcg acggcgaact tgcccttggc
      301 gcggaaggcc tggaccgcgt cgcgcatctc ctgggtcatc gccatgccgt agccgccggc
      361 gccgatgtgg ccgacgaggc cgacgacgcg gtcgtcctcg ccggcgcgct cgagcgcggc
      421 gaccagctcg gcgacgttgg cgtcgccgcc cccgaagatc cgcgagacgg ggtcggtgct
      481 gccgtactcg acgaagctgc gctcgaagtc gatctcgatg atcgtcttcg tcgggacctt
      541 cccaccgccg acggcgcagg tgaaggcgac gaagatcagc agcagcacga agagcgcggt
      601 ggtgatgccg ccgatgaccg tgagggagcg ggagagaaag gtgacgaagc ggcggcgggg
      661 gcgtgcggcg gtcatggggt ggtctcgtcg tgtgcgtcga ggccatcgag ggagtcgagg
      721 gagtcgatcg cgggcttgcg tgagaccgcg gcgccctcgc gcaatgtttc gccgagcgac
      781 ttggcccagc cctcgaggtc gccgagcgtg ctctcgagcg cgggcttgga cagctgcagg
      841 ccggacatgg tgtcgcgcag catctccttg gcgcggcgca ggcggctctt gacggtgccg
      901 gacgggatct cgagcacggt cgcgacctcg gtcgcggaca tgttctccca gaagtgcagc
      961 tcgagggcga tcaggaggac gatcggcagg cggcgcaggg cctcgtgcag gatcttctgc
     1021 tccatgtgct tggcgacgac cgtgctcggc gaggggtcga ggtcgtagaa cgaccgcgac
     1081 tcgaggtcga cctggttgtc catcttgcgg cgccggcgat agtgcgcgag cagctcgagg
     1141 cgggcgacgc cgaacaggta ggtgcgaaag ctcgaggcgc cgcggaagcg gtcgcgggtc
     1201 tcgacgcagg cgagcagggt gcgctgcatg acctcgtcgg tgtcgtcgcc gaccttgttg
     1261 cgcaggaacc ggtggatgct ctcgaagtgg cgatcgaaga gctggctgcc ggcctgcaag
     1321 tcaccgccgc gccaggcctc gaggagggcg aaatcgtcga ccatcgcgcg tcaggctaac
     1381 acggggcgga tggccggccg caggagcagc ctcacggggc gagcacgtcg gcgaggcgcc
     1441 cggggtcctg gacgccgagg tctcgcaggg cccgggcgcc gtcggggtcg cgcaggcgga
     1501 tcagggcgac ggcgtggtgg atggcgcggc cgctggcggc gaacttgccg g
//
LOCUS       DASWNJ010001359         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_272809, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001359 DASWNJ010000000
VERSION     DASWNJ010001359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272809"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VGB85_22655"
     CDS             <1..254
                     /locus_tag="VGB85_22655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136908.1"
                     /translation="LARAMDEGFRLVRRLGNTITPASMVPLSRLPTPVCAALLWGLSR
                     SAAIRKAGAAGPGEPRTLIDMMAAAAPGRTPALLAVRPA"
     gene            complement(329..1318)
                     /locus_tag="VGB85_22660"
     CDS             complement(329..1318)
                     /locus_tag="VGB85_22660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136909.1"
                     /translation="MIVEWPGADRGSLEHQFRTGVVAVRYEGCALEVLHACRVDGRYT
                     YAGFNRKRDTVVIQNIDELYTLMPSGAPRLESTLVRGQELHVDMTLVGMYTAGRTSFT
                     RDQLAGQCDAATHVVAAAQVGAFSFYSGAAMAFGENVGSREVINTDGDQARCDAATST
                     DSAPPEGCGGLLRLELVPLSAATEPVAVEVPRARAAKRDAGARSRDPKKHRWDHAAAA
                     SAASFWIGSMLFLGGIVSGGFYIAYKGRANNLAWEMKEHKSGSATWQELAERRRPYEM
                     RAKVSGWTGIGAWLLGGPLIVIGSTTGSGGLVRAGVTPIVQRTYGGLSLTFQF"
     gene            1605..>2264
                     /locus_tag="VGB85_22665"
     CDS             1605..>2264
                     /locus_tag="VGB85_22665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136910.1"
                     /translation="MFLVRSPAPALAPFVAGYWFVEDIAGAHEGLPIRTSPHPGAVLS
                     VNIGRPNAVEGGPLVPGASLLGVQTAARGWRSWSDTYFVMAMLTVRGLARLFPHTGPG
                     SADALLDLGAMIGDAASHALAADVGAAWRPGAIAGQLDRWLLARLATVEPAPGLARFA
                     AAAEVLRAGGTVEHAARHAEVTRRQLHRWFCQHIGVGPKQLMDLERFQASLQAVQREQ
                     GD"
BASE COUNT          298 a          822 c          824 g          320 t
ORIGIN      
        1 agctcgcgcg ggcgatggac gagggctttc gcctcgtacg tcggctcggc aacacgatca
       61 cgcccgcctc gatggtgccg ctgagccgct tgccgacgcc ggtctgcgcg gcgctgctgt
      121 ggggcctcag caggtccgcg gcgatccgca aggcgggcgc cgcggggccc ggtgagccgc
      181 gcacgctgat cgacatgatg gccgcggcgg cgcccggccg cacgcccgcg ctgctcgccg
      241 tgcggcccgc gtgaaacggg cgggtcgccc ccggggtccg gagccgctcg gccagctgct
      301 cgcgctgcgg cgccgggccg cgcggacctc aaaactgaaa cgtcaggctg agaccgccgt
      361 aggtccgctg gacgatcggc gtcacgccgg cgcgaacgag gcccccggat ccggtcgtgc
      421 tgccgatgac gatcagcggc ccaccgagga gccaggcgcc gatgccggtc cacccgctga
      481 ccttggcgcg catctcgtag ggccggcgcc gctcggccag ctcctgccag gtcgcgctgc
      541 cggacttgtg ctccttcatc tcccaggcga ggttattggc gcggcccttg taggctatat
      601 aaaacccgcc gctgacgata ccgccgagga acagcatcga gccgatccag aaggatgcgg
      661 ccgaggccgc ggccgcgtgg tcccaccggt gcttcttggg gtcgcggctg cgtgctccgg
      721 cgtcacgctt cgccgcgcgc gcgcgcggga cctcgaccgc gacgggctcg gtggccgcag
      781 acaggggcac cagctcgagc cgcagcagcc cgccacagcc ctccggcggc gcgctgtcgg
      841 tcgaggtcgc cgcgtcgcac ctggcctgat caccgtcggt gttgatcacc tcgcggctcc
      901 cgacgttctc gccgaacgcc atcgccgcgc cgctgtagaa cgagaacgcg ccgacctgcg
      961 cggcggcgac gacgtgggtc gccgcgtcac attggcccgc gagctgatcg cgggtgaagc
     1021 tcgtccggcc ggcggtgtac atgccgacga gggtcatgtc gacgtgcagc tcctggccgc
     1081 gcaccagcgt cgattcgagt ctgggggcgc cgcttggcat gagcgtgtag agctcgtcga
     1141 tgttctggat caccaccgtg tcgcgcttgc ggttgaagcc ggcgtaggtg taccgaccat
     1201 cgacgcgaca ggcgtgcaac acctcgagcg cgcagccctc gtagcgcacc gcgacgacgc
     1261 cggtgcgaaa ctggtgctcg agcgagcccc ggtccgcgcc gggccactcg acgatcaaag
     1321 gccgcagctg actcggtacg atcctacagt gcggtagacc ggcggccccc ggcgtcggca
     1381 gctcgggtgg cgccggcgtt cggtccgagc gacagcccgc gagcagcagc gcgagcgcga
     1441 cgccggatgc cacaaagcgc gggttcgacc tcgatatcaa tcgcaccgag cctacgcgcc
     1501 gcgcaggcgg tcggcaaaca aaacgggcca cagaccgccc gcgggccgat cgtccgcggt
     1561 ttcagcggct cgacgcgcgc gtcgcgagcg ggcacgatcc agcggtgttc ctcgtccggt
     1621 ctccggcccc cgcgctcgcg cccttcgtcg ccgggtactg gttcgtcgag gacatcgccg
     1681 gtgcgcacga gggtctgccg atccgcacca gcccgcaccc tggcgctgtg ctctcggtca
     1741 acatcgggcg ccccaacgcg gtcgagggcg ggccgctggt gccgggcgcg tcgctgctcg
     1801 gcgtgcagac cgcggcccgc gggtggcgtt cgtggtcgga tacatacttc gtgatggcga
     1861 tgctgacggt gcgggggctc gcgcggctgt tcccgcatac cggccccggc tccgccgacg
     1921 cgctgctcga cctcggcgcg atgatcggtg atgcggcgtc ccacgccctc gcggcggacg
     1981 tcggcgccgc gtggcggccg ggtgcgatcg cggggcagct cgaccgctgg ctgctcgcgc
     2041 ggctcgccac cgtcgaacca gctcccggcc tcgcccgctt cgccgccgcg gcggaggtgc
     2101 tacgcgcggg cggcacggtc gagcacgccg cccggcacgc ggaggtgacg cggcgtcagc
     2161 tgcacaggtg gttttgtcag cacatcgggg tcggccccaa acagctgatg gatctggaga
     2221 gattccaggc gagcctccag gcggtgcagc gcgagcaggg tgat
//
LOCUS       DASWNJ010001360         3820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_94321, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001360 DASWNJ010000000
VERSION     DASWNJ010001360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94321"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1486)
                     /locus_tag="VGB85_22670"
     CDS             complement(<1..1486)
                     /locus_tag="VGB85_22670"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0136911.1"
                     /translation="MTRPGEPLADVDATRAPATGPLWQMPASQLGATTASAELDATSR
                     AAETLARMERRASWPGIAQTRGTAAAGPGAPTMPERLGRYMVIKELGAGGMGVVYKAF
                     DPDLDRKVALKLLLGQATPQARARLLREAQAMAKLSHPNVVPVFDVGVVDGQVFVAMD
                     FVDGMTLSVWMESRHPWQDVVEVFVQAGRGLAAAHAVGLIHRDFKPDNVLLRRDPATG
                     ELQAQVADFGLARRDDETGESITRPEPAVVMQSRHSLLDQGLTRIGAVIGTPTYMSPE
                     QHVGATVDARTDQFSFCVALFEALYGERPFTGETLEALAIAASGPRRPPPDRKVPGWL
                     YRLCLRGMQPAREARFPDMEALLLEISRRRAQGRHRWFAAAGGVAVIGALAAVLVNRG
                     QPAEQRCAGGPARVAGVWDEAVQARAEQAFAATGVVYAADAWKAARGHVDRYGEAWAT
                     MYRDSCEATDRGEQSAELMDLRTACLGRALQDLSALARLYVAADK"
     gene            1891..3723
                     /locus_tag="VGB85_22675"
     CDS             1891..3723
                     /locus_tag="VGB85_22675"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY0136912.1"
                     /translation="MLRRLSISFLAAIPLIPLVLSGCEGGGRDPGESAGEDGEFPVPD
                     PVDPAAPADPGEYLMGIEGEFMAASEEFNVPVDVLKAVGYVESQWQMVAGEVEFEGIM
                     PAFGAMALRGPNLARGATLLGVEEDAVKQTRQLNIRAAAAVLSEKADIKGIDRANLGA
                     WAPAIAELSGIEPESAAAMSYIHQNVYSLISNGLVVTDLAGNVISEIKPKTGVLPDYP
                     DPNGPVLAANPDFAGATFRASPNFSARPAGTTGQIRIVIIHTCEGAYAGCWGWLAQSK
                     SGVSAHYVVKEDGSEITQLVKEKDKAWHIAAKYKKSLNGGMFGEVEGSSGNNFTVGIE
                     HAGFGSQKSWNPNLIDQSAKLVCDITRRQGIPRDKYHIVGHGQLQPYNRTDPGPNWPW
                     ATYFNKIIAACGEAPQDDPPPPPPPEPPPPHEDDPPPPPPPDDSTTSGGDEGGSTTDP
                     YPDDPPPDDPPPDVPPPADASEIIIDNDNSNNASNADYFAPAAWTATASTPGFYGNNY
                     TYAATTTEDNGAEHWFYLETGGTYNVDVWYTAGANRAASAAIVAFDAANTEIGYQTIN
                     MQQGGKAWTPAGTYTFTAGWNMVMVSRWADAGFVVVSDAVRVRP"
BASE COUNT          574 a         1558 c         1181 g          507 t
ORIGIN      
        1 gcttgtccgc ggcgacgtac agccgcgcca gcgcggacag gtcctgcagc gcgcgcccga
       61 ggcaggccgt gcgcaggtcc atcagctccg ccgactgctc gccgcggtcg gtcgcctcgc
      121 agctgtcgcg atacatcgtc gcccacgcct cgccgtaacg atcgacgtgc ccgcgcgcgg
      181 ccttccacgc gtcggccgca tagaccacgc cggtcgcggc gaacgcctgc tccgcgcgcg
      241 cctgcacggc ctcgtcccac accccggcga cgcgcgccgg gccgccggcg cagcgctgct
      301 ccgcgggctg accccggttc accagcaccg ccgccagcgc gccgatcacc gccaccccgc
      361 cggccgccgc gaaccagcgg tgccggccct gcgcgcgccg ccgcgagatc tcgagcagca
      421 gcgcctccat gtccgggaac ctcgcctcgc gcgccggctg catcccgcgc aggcacaggc
      481 gatacagcca gcccggcacc ttgcgatccg gcggcggccg ccggggtccg ctcgccgcga
      541 tcgccagcgc ctcgagcgtc tcgccggtga acggacgctc gccgtacagc gcctcgaaca
      601 gcgcgacgca gaagctgaac tggtcggtgc gcgcgtcgac cgtcgcgccg acgtgctgct
      661 ccggcgacat gtacgtcggc gtcccgatca ccgcgccgat gcgcgtcagc ccctggtcga
      721 gcaggctgtg ccgcgactgc atcaccaccg cgggctccgg ccgcgtgatc gactcgccgg
      781 tctcgtcgtc gcggcgcgcc agcccgaagt ccgcgacctg ggcctgcagc tcccccgtcg
      841 cgggatccct gcgcagcagc acgttgtcgg gcttgaagtc gcggtggatc agcccgaccg
      901 cgtgcgccgc cgccaggccg cgccccgcct gcacgaacac ctcgacgacg tcctgccacg
      961 ggtgccgcga ctccatccac acgctcagcg tcatgccgtc gacgaagtcc atcgccacga
     1021 acacctggcc gtcgaccacc ccgacgtcga acaccggcac cacgttcggg tgcgacagct
     1081 tggccatcgc ctgcgcctcg cgtagcagcc gcgcgcgcgc ctgcggggtc gcctgcccga
     1141 gcagcagctt gagcgccacc ttgcggtcga ggtccgggtc gaaggccttg tagaccaccc
     1201 ccatcccgcc ggccccgagc tccttgatca ccatgtagcg accgagccgc tccggcatcg
     1261 tcggcgcccc cggccccgcc gcggcggtcc cccgcgtctg cgcgatgccc ggccacgacg
     1321 cccggcgctc catgcgtgcc agcgtctcgg ccgcgcggct cgtcgcgtcg agctctgccg
     1381 acgccgtcgt cgcgccgagc tgcgacgcgg gcatctgcca cagcggtccc gtcgcgggcg
     1441 cccgcgtcgc gtcgacgtcc gcgagcggct cgcccgggcg tgtcacatcg acgcccggcg
     1501 cttccccggc ccgcgtcgcg tcgacgtccg gcgctcctgc atccgcccgc gtcgcatcga
     1561 gatccctccg ctctgccatc gttcccggcg gactctaaca tgcgacgtgc agcactacgc
     1621 gggcgcggct acgtcacgca cccacatgca gacagccgcg tcacgaacgc tcgccgatcg
     1681 atcatccgcc gcgccgcctg agccccctcg ccgcattcgc cgacatgccg gcccgcgccg
     1741 taacagccgc atacctcgcc gcacgcacat gcggggctcg caacccccga tgcatcgcgt
     1801 ctcacttcac cctcgtcgcc tgctcgcaac agcgcgctca tcggcgccgc ttgtgggcgc
     1861 ttcacggctt cgatatagaa gtatgtgatg atgctgcgcc gactatcgat ctccttcctg
     1921 gccgcgatcc cattgatccc gctggtgctg tccggctgcg aaggcggcgg acgtgacccc
     1981 ggcgagagcg cgggcgaaga cggcgagttc ccggtccccg acccggtcga ccccgccgcg
     2041 cccgccgatc ccggcgagta tctgatgggt atcgagggcg agttcatggc cgcctccgag
     2101 gagttcaacg tccccgtcga cgtgctcaag gccgtcggct acgtcgaatc gcagtggcag
     2161 atggtcgccg gcgaggtcga gttcgagggc atcatgcccg ccttcggcgc catggcgctg
     2221 cgcggaccca acctcgcccg cggcgccacc ttgctcggcg tcgaggagga cgcggtcaag
     2281 cagacgcgcc agctcaacat ccgcgccgcc gcggcggtgc tcagcgagaa ggccgacatc
     2341 aagggcatcg accgcgccaa cctcggcgcg tgggccccgg cgatcgccga gctctcgggc
     2401 atcgagcccg agtcggccgc cgcgatgagc tacatccacc agaacgtcta ctcgctgatc
     2461 tccaacggcc tcgtcgtcac cgacctcgcc ggcaacgtca tctcggagat caagcccaag
     2521 accggcgtcc tgcccgacta ccccgatccc aacggccccg tgctcgcggc caacccggac
     2581 ttcgccggcg ccacctttcg cgcctcgccg aacttcagcg cgcgcccggc cggcaccacc
     2641 ggccagatcc gcatcgtcat catccacacc tgcgagggcg cctacgccgg ctgttggggc
     2701 tggctcgcgc agtccaagtc cggcgtcagc gcccactacg tcgtcaaaga ggacggctcg
     2761 gagatcaccc agctcgtcaa ggagaaggac aaggcctggc acatcgccgc caagtacaag
     2821 aagtcgctca acggcggcat gttcggcgag gtcgagggct cgagcggcaa caacttcacc
     2881 gtcggcatcg agcacgccgg cttcggcagt cagaagtcct ggaacccgaa cctgatcgac
     2941 cagtcggcca agctcgtctg tgacatcacc cggcgccagg gcatcccgcg cgacaagtac
     3001 cacatcgtcg gtcacggcca gctccagccg tacaaccgca ccgacccggg cccgaactgg
     3061 ccgtgggcga cctacttcaa caagatcatc gccgcctgcg gcgaggctcc gcaggacgac
     3121 ccgccgcccc cgcccccgcc cgagccgccg ccgccgcacg aggacgaccc gccgcccccg
     3181 cccccgccgg acgacagcac cacctccggt ggcgacgagg gtggctcgac caccgacccg
     3241 tacccggacg acccgccgcc cgacgatccg ccgcccgatg tgcccccgcc ggccgacgcc
     3301 agcgagatca tcatcgacaa cgacaacagc aacaacgcct cgaacgccga ctacttcgcg
     3361 cccgcggcgt ggaccgcgac tgcatcgacg ccgggcttct acggcaacaa ctacacctac
     3421 gcggccacca ccacggagga caacggcgcc gagcactggt tctacctcga gaccggtggc
     3481 acctataacg tcgacgtctg gtacaccgcc ggcgccaacc gggccgccag cgccgcgatc
     3541 gtcgcgttcg acgccgcgaa caccgagatc ggctaccaga cgatcaacat gcagcagggc
     3601 ggcaaggcct ggacccccgc gggtacctac accttcaccg ccggctggaa catggtcatg
     3661 gtgtcccgct gggccgacgc cggcttcgtc gtcgtctccg acgccgtccg cgtccgcccg
     3721 taacaccggc tgatgcgcac gcctgccggc gcccggtcct ccggtcgccg gcagtcgctt
     3781 tttaatacct tacgcgtcgg tcgcgtcgtc gagcgcgtcc
//
LOCUS       DASWNJ010001361         6522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_201494, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001361 DASWNJ010000000
VERSION     DASWNJ010001361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6522
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201494"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..862)
                     /gene="lexA"
                     /locus_tag="VGB85_22680"
     CDS             complement(233..862)
                     /gene="lexA"
                     /locus_tag="VGB85_22680"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEY0136913.1"
                     /translation="MKPSLTHRQQQALDFISSCLDENGYPPTLREIGEHMGIKSTNGV
                     NDHLKALERKGYLMREELKSRALRPVEQPENARKLEVPILGRVAAGQPILAEENVVDR
                     INIDKRFLGSYPSREVFGLIVQGESMIEDGIFDGDYLFIRRQSTAERGEIVVAMIEGE
                     ATCKRYYLEGEKIRLQPANAAMEPIYVHRGDGRSFDILGKVVGVYRNLH"
     gene            1167..1514
                     /locus_tag="VGB85_22685"
     CDS             1167..1514
                     /locus_tag="VGB85_22685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136914.1"
                     /translation="MLASAVLGVVVAVAMQSLVEATPTRTGTWAAGLPGRLPPRSVPV
                     QAPPFTASEAEALHDEAERLTPAAVALDEQAALRWWPLRGELEAAADDPATSEHVRNE
                     LRATLEALGRVGL"
     gene            1626..2606
                     /locus_tag="VGB85_22690"
     CDS             1626..2606
                     /locus_tag="VGB85_22690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose phosphate epimerase"
                     /protein_id="HEY0136915.1"
                     /translation="MLRPGLSWIASLVLVSAACSDKEPAETTKATTEPADTTGGAELT
                     SSGGETPATNPGSATEAASDPTTTGATASGTSNPDPTGTTNNSDPSDPTNAESGGFID
                     RPDGGIAGQCDPRAQDCPEGEKCSAVSIAAGDPWGANTCVPLKGDGQVGDPCDIEDGK
                     YTGVDNCDLGLICLLTDDEGKGGACVEFCDTNDACPDTPGAKCVTYNDGSLPICLNSC
                     DPLIQDCPEGQACYNSAGDNFVCFKESAMPGEGTPGDECAYINQCQKGSFCAAATALA
                     DCPTPSCCTPYCPVSGGNAPCNMGEDCVAFFAEGMSPPGLEDVGVCAIPG"
     gene            2801..2959
                     /locus_tag="VGB85_22695"
     CDS             2801..2959
                     /locus_tag="VGB85_22695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136916.1"
                     /translation="MRVLISAIITGFGLKLGTDIYKYVKQRVGVFGDDQKDGGDEAVD
                     QAGGTAGA"
     gene            2956..3285
                     /locus_tag="VGB85_22700"
     CDS             2956..3285
                     /locus_tag="VGB85_22700"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY0136917.1"
                     /translation="MSEELQIDDLVPGTGPAVRSGQRVSVHYTGWLADGTKFDSSYDR
                     NRPFAFKLGASEVILGWEDGIVGMQVGGKRRLTVPPNLAYGRRGMRGVIPPNATLVFE
                     VELLAIP"
     gene            complement(3290..4243)
                     /locus_tag="VGB85_22705"
     CDS             complement(3290..4243)
                     /locus_tag="VGB85_22705"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY0136918.1"
                     /translation="MLLERALRGLVILTIAGTGLASLLGRFGAWAWWLDLFAHFRVQY
                     FLALVFALVLAAVVRRRRLLVIAGALLVLELATICAVAGLRDRSSAGPSLRLVHFNLL
                     SSNHRHAEVQRWISDSGADLVFVQEVIPRWADVLAATPGHAVIEVVDRDDNFGLAILA
                     RSDSGVEVLSHERVVFAGLPALAVRLRHEGRVLAVLSLHTLPPMSAAHTATRDTQLIA
                     AAQWAAAQKAAGAAAVVLGDFNATPFSSGVAPLAAAGLRDSLDVRSLLFAGSWPGLSW
                     PLRIAIDHCWHDERLVTVERAIGPALGSDHRPLTVTLAWAR"
     gene            4425..6521
                     /locus_tag="VGB85_22710"
     CDS             4425..6521
                     /locus_tag="VGB85_22710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEY0136919.1"
                     /translation="MSTKTLAIKVYHGEQLVDTQTLSQDVIKIGKLKSSHLCLDDETV
                     ARMHAVIEVSGDEVRVIDLGSATGTTLNGARIDKNAALSDGDVLTFGPYRLEVGFVRQ
                     APVAAAAAPGHAPIHAPMHAAPLAAVPAPMMQRQPVQIDASEFEVQNGSRVAEVVAMY
                     GNTVLDVQHVGQLKNKKSQAPMFLAIGGLMLVAGTGIFLSEVKQDWAGYQDKLAEANE
                     TGGLRPQKPGNGLGALGVALALLGVVPFGFGLIRLSDFVNRSYTIGESHGASFHVPPQ
                     GLPDGSAFPLVRGSDHEFSLNFTQNMTGEVTFDGQSIPLGQLVSSGRAGSTGSSYTFP
                     LPPGANCRVKYNDVTFFVNSVAPGAVIKGGSDTDKPFWFYNGASFAVIGSLLVLTHLI
                     PNEAGELSLDDMGEENRFVGYLNTPDKPPEEEEPPPEDQENTDEEAGGTGQRHKGEEG
                     KMGKPTSKSKSGLYAMKGPKDAIPQMARSFDPEMAARQAGILGVMQQQSGHFLASPYG
                     GAFAVGNDDADVWGGLTGTEIGEAYGVGGLGLVGTGRGGGGTGEGTIGLGNTGLIGKG
                     GGGGSGSGYGRGAGAGFGGRGTRVPTVRQAKAEVQGALDKDIIRRIVRAHINEVRYCY
                     NQALARDPNAKGRVAVQFTIGGTGKVPSAVVQETTMKDAGVGNCIAQAVKRWTFPKPE
                     GGGSVIVTYPFVLEPG"
BASE COUNT         1048 a         2382 c         2187 g          905 t
ORIGIN      
        1 gggctatctc ggcgggacga caccggcatc actgtagcgg aagtccctcg ccggggcctg
       61 tggagcgcga acgcggcgcc tcagcggaga acgcgctggc gcctgcgcgg gcctgtctgc
      121 gggccctatg aggcacgcag ctggcataca gccggcccgc agccggacac gcagccgggc
      181 gcgcagccgg gcgatgcccg cgtgtgagct ggccgacccg gcgaggaaat cctcagtgga
      241 ggttgcggta cacgccgacg accttgccga ggatgtcgaa gcttcgaccg tcgccgcgat
      301 gcacgtagat cggctccatc gccgcgttgg cgggctggag gcggatcttc tcgccctcga
      361 ggtagtagcg cttgcaggtc gcctcgccct cgatcatcgc cacgacgatc tcgccgcgct
      421 cggcggtcga ctggcggcgg atgaacaggt aatcgccgtc gaagatgccg tcctcgatca
      481 tgctctcgcc ctgcacgatc aggccgaaca cctcgcgcga cgggtacgac ccgaggaagc
      541 gcttgtcgat gttgatgcgg tcgaccacgt tctcttcggc caggatcggc tgaccggcgg
      601 ccacgcgacc gaggatcggg acctcgagct tgcgggcgtt ctcgggctgc tcgaccgggc
      661 gcagcgcgcg gctcttgagc tcctcgcgca tcaggtaacc cttgcgctcg agcgccttga
      721 ggtgatcgtt gaccccgttg gtcgacttga tccccatgtg ctcgccgatc tccctgagcg
      781 tgggcgggta gccgttttcg tccaggcagg agctgatgaa gtcgagcgct tgctgttggc
      841 gatgtgtcag gctgggtttc acggctgggc ctccgacgtc gcggttgcgc cgaatgcgct
      901 tctgcgacac tgaacgggtg tatacaaaca atttgcgcag gtcaagcttt tgcaccgcca
      961 cgcggacccc attggcgccc gaaatttggc gatcgccggc atgacagcca acctccccct
     1021 cgatgcagcc acaggccccg atcgaacgtc gttccgatcg ccaggaccgg cgccggccag
     1081 gaccgacgac gtggcccgcc ccgctcgagc gccgcagcgg cgagcgccgc cgactgccgc
     1141 gctcacgctg gcagcagtgg ccgtccttgc tggcttccgc ggtcctcggg gtcgtcgtcg
     1201 cggtcgcgat gcagtcgctc gtcgaggcga cgccgacccg caccgggacc tgggccgcgg
     1261 gcctgcccgg gcgcctgccc ccgagatcgg tgcccgtgca agcacctccc ttcaccgcgt
     1321 ccgaggccga ggccctgcac gacgaggccg agcgcctgac gcccgccgcg gtcgccctcg
     1381 acgagcaggc ggcgctgcgc tggtggccgc tgcgtggcga gctcgaagcg gccgccgacg
     1441 accccgcgac ctccgagcac gtgcgcaatg agcttcgcgc gaccctcgag gccctcggcc
     1501 gcgtcggcct ctaggtcctc ggacgcgacg accccggtcc cggccgcgaa gtttcatccg
     1561 ccgaaccaac taccgggatt tttacccgcg atttttgtta gggtcgaagc gtggagagac
     1621 cgctcatgct tcgacctggc ttatcgtgga tcgcgtccct ggtgctcgtg tcggcggcct
     1681 gcagcgacaa ggaacccgcc gagactacca aggccaccac cgagcctgcg gacaccaccg
     1741 gcggcgccga gctcacctcc agcggcggcg agaccccggc caccaaccct ggcagcgcga
     1801 ccgaggcggc cagcgacccg acgaccaccg gcgccaccgc atcgggcacg agcaaccccg
     1861 acccgaccgg caccaccaac aacagcgacc cctccgaccc gaccaacgcg gagagcggcg
     1921 gcttcatcga tcgccccgac ggcggcatcg ccggccagtg cgacccccgg gcccaggact
     1981 gtcccgaggg cgagaagtgc tcggccgtgt cgatcgcggc gggcgatcca tggggcgcca
     2041 acacctgcgt gccgctcaag ggcgacggcc aggtcggcga cccctgcgac atcgaggacg
     2101 gcaagtacac cggcgtcgac aactgcgacc tcggcctgat ctgcctgctg accgacgacg
     2161 agggcaaggg cggcgcctgc gtcgagttct gcgacaccaa cgacgcctgc cccgacaccc
     2221 ccggggccaa gtgcgtgacc tacaacgacg gctcgctgcc gatctgcctc aacagctgcg
     2281 acccgctgat ccaggactgt cccgaaggac aggcctgcta caactccgcc ggcgacaact
     2341 tcgtgtgttt caaggagagc gccatgccgg gcgagggcac gccgggcgac gagtgcgcgt
     2401 acatcaatca gtgccaaaag ggctcgttct gcgcggccgc gacggcgctc gccgactgcc
     2461 cgaccccgag ctgctgcacc ccgtactgcc cggtctccgg cggcaacgcc ccgtgcaaca
     2521 tgggtgagga ctgcgtcgcc ttcttcgccg agggcatgtc ccctccgggc ctcgaagacg
     2581 tcggtgtctg cgcgatcccg gggtgagtcg agcgctcgcc ggatcctgcg cggtcggcgg
     2641 aggatcggcc ggcggccagg cgcctcgccg tcggccgccg ccgccaaacc cggcctgatc
     2701 gccgtgcccc ggcccgaaat ccgcgatgtg gcagcgaccc ggcccggcgc ggccgggcgc
     2761 ccgcggcggc acattggcgc gggcctgcta ggctagcgcc atgcgcgttc tcatctcggc
     2821 gatcatcacc ggcttcggcc tcaagctcgg caccgacatc tacaagtacg tgaagcagcg
     2881 cgtcggcgtg ttcggcgacg accaaaagga cggcggcgac gaggccgtgg accaggccgg
     2941 gggcacggcc ggcgcttgag cgaagaactg cagatcgacg atctcgtccc ggggaccgga
     3001 cctgctgtcc gcagcggcca gcgggtctcc gtccattaca cggggtggct cgccgacggc
     3061 accaagttcg acagctcgta cgaccgcaac cgtccgttcg ccttcaagct cggtgccagc
     3121 gaggtgatcc tcggctggga agacggcatc gtcggcatgc aggtcggcgg caagcgccgc
     3181 ctgaccgtcc ccccgaacct cgcctacggc cgtcgcggca tgcgtggcgt catcccgccg
     3241 aacgcgaccc tcgtcttcga ggtcgagctg ctggcgatcc cgtgacttcc taccgggccc
     3301 acgccagcgt gaccgtcagc ggccgatgat ccgagcccag cgccggaccg atcgcccgct
     3361 cgacggtgac gaggcgctcg tcgtgccagc aatgatcgat ggcgatccgc agcggccacg
     3421 atagtcccgg ccagctgccg gcgaacagca gcgagcgcac gtccagcgag tcgcgcagcc
     3481 ccgccgcggc gagcggcgcg acgccgctgc taaatggcgt cgcgttgaag tccccgagca
     3541 cgaccgcggc ggcccccgcg gccttttgtg ccgcggccca ctgcgccgcg gcgatcagct
     3601 gggtgtctct cgtggccgtg tgcgccgcgg acatcggcgg cagcgtgtgc aggctgagca
     3661 ccgccagcac gcgcccctcg tgacgcagcc gcaccgcgag cgcgggcagc ccggcgaaca
     3721 cgacgcgctc gtgcgagagg acctcgacgc cgctgtcgct gcgcgcgagg atcgccagac
     3781 caaaattgtc gtcgcggtcg acgacctcga tcaccgcatg cccgggcgtg gccgcgagca
     3841 cgtcggccca gcgtgggatc acctcctgca cgaacaccag gtccgccccg ctgtccgaga
     3901 tccaccgctg cacctccgcg tgtcgatggt tgctcgacag caggttgaag tgcaccagcc
     3961 gcagcgacgg ccccgcggag gacctgtccc gaaggccagc cacggcgcag atcgtcgcca
     4021 gctcgagcac cagcagggcc cccgcgatca cgagcagacg gcgccggcgg acgaccgccg
     4081 cgagcaccag cgcgaacacc agcgcgagga agtactgcac gcgaaaatgc gcgaacagat
     4141 cgagccacca cgcccacgcg ccgaatctcc cgagcaagct ggcgaggccg gtcccggcga
     4201 tcgtcaggat gacgaggccc cgcagcgcgc gttcgaggag caccgcgcga gcataaccgg
     4261 ccgagcctcg cggtcgcgcg ccccctcagg cgatgtcgcg ccaggcgggc cgcagggccg
     4321 tgtgtgggca cacgggcggc accagtgaac cgttgatcgc cggggaaccc cggccggggg
     4381 gcgctgtctg tgccggagta actgctggcc caaggacccg gaaaatgtcc accaagactc
     4441 tcgcaatcaa ggtttatcac ggcgaacaac tcgtcgacac ccagaccctc tcacaagacg
     4501 tcatcaagat cggcaagctc aagagcagcc acctgtgtct cgatgacgag accgtggcgc
     4561 gcatgcatgc cgtcatcgag gtctccggcg acgaggttcg cgtcatcgac ctcggcagcg
     4621 ccaccgggac caccctcaac ggcgcccgta tcgacaagaa cgcggcgctc tccgacggcg
     4681 acgtcctgac cttcgggccc taccgcctcg aggtcggctt cgtccgccag gcccccgtcg
     4741 ccgcggccgc tgcaccgggc catgctccga tccacgcgcc gatgcacgcc gccccgctgg
     4801 ccgcagtgcc ggcgccgatg atgcagcgcc agcccgtgca gatcgacgcg agcgagttcg
     4861 aggtccagaa cgggtcgcgg gtcgccgagg tcgtggcgat gtacggcaac accgtgctcg
     4921 atgtgcagca cgtcggtcag ctcaagaaca agaagtcgca ggcgccgatg ttcctggcga
     4981 tcggcgggct catgctcgtc gccggcaccg gcatcttcct cagcgaggtc aagcaggact
     5041 gggcgggcta tcaggacaag ctcgccgagg ccaacgagac cggcggtctg cgcccgcaaa
     5101 agcctggcaa cggcctcggc gctctcggcg tcgcgctcgc gctgctcggc gtggtgccct
     5161 tcggcttcgg cctgattcgc ctcagcgact tcgtcaaccg cagctacacg atcggcgaga
     5221 gccacggcgc ctccttccac gtgccgccgc agggcctgcc cgacggctcg gcgttcccgc
     5281 tggtccgcgg cagcgaccac gagttctcgc tcaacttcac ccagaacatg actggcgagg
     5341 tcaccttcga cggccagagc atcccgctcg gccagctggt cagctcgggc cgggccggct
     5401 cgaccggctc gtcgtacacc ttcccgctgc ctccgggtgc caactgccgc gtcaagtaca
     5461 acgacgtcac cttcttcgtg aactcggtcg ccccgggcgc cgtgatcaag ggcggcagcg
     5521 acaccgacaa gccgttctgg ttctacaacg gtgcgtcctt cgccgtgatc ggctcgctgc
     5581 tggtgctcac gcacctgatc cccaacgagg ccggtgagct gagcctcgac gacatgggcg
     5641 aggagaaccg cttcgtcggc tacctcaaca cgcccgacaa gccgccggag gaggaggagc
     5701 cgccgccgga ggatcaggag aacaccgacg aggaggccgg tggtacgggc cagcgtcaca
     5761 agggcgagga gggcaagatg ggcaagccca cctccaagtc caagtctggc ctctacgcga
     5821 tgaagggccc gaaggacgcg atcccgcaga tggcccgctc gttcgacccc gagatggccg
     5881 cgcgtcaggc cggcatcctc ggcgtcatgc aacagcagag cggtcacttc ctcgcctcgc
     5941 cgtacggcgg cgcgttcgcg gtcggcaacg acgacgcgga cgtgtggggc ggtctgaccg
     6001 gcaccgagat cggcgaggct tacggcgtcg gcggcctcgg cctggtcggc accggccgcg
     6061 gcggtggtgg taccggtgag ggcacgatcg gcctcggcaa caccggcctg atcggcaagg
     6121 gcggcggcgg tggctccggc tccggctacg gccgtggcgc aggcgccggc ttcggcggtc
     6181 gtggcacccg cgtgccgacg gtccgtcagg ccaaggccga ggttcagggc gcgctcgaca
     6241 aggacatcat ccgtcgtatc gtccgcgcgc acatcaacga ggtccgctac tgctacaacc
     6301 aggcgctggc ccgtgacccc aacgccaagg gccgcgtcgc cgtccagttc acgatcggcg
     6361 gcaccggcaa ggtcccctcg gccgtcgtcc aggagaccac catgaaggac gccggcgtcg
     6421 gcaactgcat cgcgcaggcc gtcaagcgct ggaccttccc caagcccgag ggcggcggca
     6481 gcgtcatcgt gacctacccc ttcgtgctcg agccgggctg at
//
LOCUS       DASWNJ010001362         3818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_406753, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001362 DASWNJ010000000
VERSION     DASWNJ010001362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406753"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..765
                     /gene="pth"
                     /locus_tag="VGB85_22715"
     CDS             178..765
                     /gene="pth"
                     /locus_tag="VGB85_22715"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY0136920.1"
                     /translation="MDTAPWLIVGLGNPGPRYARTRHNIGFMAVDAWVGELASGSAPP
                     PGWSEKWKGQTTALKCGSTRCVVLKPQTYMNRSGFSVVPAAQFHRVPPPQIVVVHDEI
                     DFPLGRMAIKKGGGHGGHNGLRDIVAQLGSAEFLRIRLGVGRPVHGEVADYVLSDFGS
                     DEQGPAADMVERAVKALTCMMTDGVVMAMNKFNTT"
     gene            821..3103
                     /locus_tag="VGB85_22720"
     CDS             821..3103
                     /locus_tag="VGB85_22720"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEY0136921.1"
                     /translation="MDIPIETLLYAVPACGGLALLYAFFTTAWITKQDPGNLRMQKIA
                     DQIFRGAMAFLAAEYKVLAIFVVIIAGLLAVSADETSSPLVGLSFVAGALCSALAGFF
                     GMRVATRANVRTAAAARIGLAPALAVAFRGGAVMGMCVVGLGLVGFGTLFIVYLGQFD
                     ALQDVDGQAFQFNRVVNMLAGFAMGASSIALFARVGGGIYTKAADVGADLVGKVEAGI
                     PEDHFLNPATIADNVGDNVGDVAGMGADLFESYIGAILGTMVLGAVAISGRLEASILP
                     LLIAAAGIVMSILGTFLVRTKEGGNPQLALDAGSFGAAFIMLPISWGIIYFCVMSAAG
                     HGTPMITTDLSNLAACSVNGVMDAANTAVISSDACSMSQTWALFWAIVAGLISGVAIG
                     AITGFYCSKGKRPVNSIVEQSSTGPATNIIAGIGVGFESTAIPILLIAAAIFVANYFA
                     GLYGVALAALGMLSTTGIQLAVDAYGPISDNAGGIAEMSGLPHEVRERTDNLDAVGNT
                     TAAIGKGFAIASAALAALSLFAAYRNIALKDSDLLLTDPKVLIGLLIGGMLPFLFSAM
                     AMKAVGAAAMDMITEVRRQFREIPVLREALTLVARAEKEGRDLTSEEEAQVLAAGEKT
                     EVERCVAISTKASIRRMVMPGVLALTSPVIVGFWSRNALGGLLAGTLVTGVMLAIFMS
                     NAGGAWDNAKKQVEDQKKDSSQAVNTGKGSERHKAAVIGDTVGDPFKDTAGPSLNILI
                     KLMTIVAVVIAPILVKYGGL"
BASE COUNT          572 a         1417 c         1254 g          575 t
ORIGIN      
        1 ggaagaggtc gacgacaagg ccaagaagcc ggcggccaag gccgccgcga agccggcagc
       61 agcagctgcc gcgaagccgg cggccgccgc agccgccgcg cccaaggcag ccgccgccaa
      121 gccgaagaag tgagccgccc ctgatgtggt ggcgccgcac gcgaacggag aaggccaatg
      181 gacaccgcac catggctgat cgtgggcctc gggaacccgg ggccgcgtta cgcgcgtaca
      241 cgccacaaca tcgggttcat ggcagtcgac gcctgggtcg gggagctcgc ctcgggctcg
      301 gcacccccgc caggctggtc cgagaagtgg aagggccaga cgaccgcgct caagtgcggc
      361 agcacccgct gcgtggtcct caagccgcag acgtacatga accgcagcgg gttcagcgtc
      421 gtgccggcgg cccagttcca ccgcgtaccg ccgccgcaga tcgtggtcgt gcacgacgag
      481 atcgacttcc cgctcggtcg catggcgatc aaaaagggcg ggggtcatgg aggccacaat
      541 ggcctgcgtg acatcgtcgc ccagctcggc tcggccgagt tcctgcggat ccgcctcggc
      601 gtcgggcgac ccgtgcatgg cgaggtcgcc gactacgtgc tgtccgactt cggctccgac
      661 gaacagggcc ccgccgccga catggtcgag cgcgccgtga aggccctgac ctgcatgatg
      721 accgacgggg tcgtcatggc gatgaacaag ttcaacacga cctgagcggc tccccccgcc
      781 ggggtcgcac cccctccccc gcaagaaagc accctccccc gtggacatcc caatcgaaac
      841 actcctctac gccgtgccgg cctgcggcgg cctggcgctg ctctacgcat tcttcacgac
      901 cgcatggatc accaagcagg accccggtaa cctgcgcatg cagaagatcg cggaccagat
      961 cttccgcggc gcgatggcct tcctggccgc cgagtacaag gtcctggcga tcttcgtcgt
     1021 catcatcgcc ggccttttgg ccgtgtcggc cgacgagacg tcctcgccgc tggtcggcct
     1081 gtcgttcgtc gccggcgccc tgtgctccgc gctcgccggc ttcttcggca tgcgcgtggc
     1141 gacccgcgcc aacgtccgca ccgcggccgc ggcccgcatc ggcctcgcgc cggcgctcgc
     1201 cgtcgcgttc cgcggcggcg ccgtgatggg catgtgcgtc gtcggcctcg gcctggtcgg
     1261 cttcggcacg ctattcatcg tctacctcgg ccagtttgac gccctccagg acgtcgacgg
     1321 tcaggcgttc cagttcaacc gcgtcgtcaa catgctcgcc ggcttcgcga tgggcgcctc
     1381 gtcgatcgcg ctgttcgctc gcgtcggcgg cggcatctac accaaggcgg ccgacgtcgg
     1441 cgccgacctg gtcggcaagg tcgaggcggg catccccgag gaccacttcc tcaaccccgc
     1501 gacgatcgcg gacaacgtcg gcgacaacgt cggtgacgta gccggcatgg gcgcggacct
     1561 gttcgagtcg tacatcggcg cgatcctcgg cacgatggtg ctcggggccg tggccatcag
     1621 cggccgcctc gaggcctcga tcctgccgct gctgatcgcc gcggccggca tcgtgatgtc
     1681 gatcctcggc accttcctgg tgcgcaccaa ggagggtggc aacccccagc tcgcgctcga
     1741 cgccggctcg ttcggcgcgg cgttcatcat gctgccgatc tcgtggggca tcatctactt
     1801 ctgcgtgatg tcggccgccg gccacggcac gccgatgatc accaccgacc tgtccaacct
     1861 ggcggcgtgt agcgtcaacg gcgtgatgga cgcggccaac accgccgtca tcagctccga
     1921 cgcgtgcagc atgtcccaga cctgggccct gttctgggcg atcgtcgccg gcctgatctc
     1981 cggcgtggcg atcggcgcca tcaccggctt ttactgctcc aagggcaagc gcccggtcaa
     2041 cagcatcgtc gagcagtcga gcaccggccc ggcgaccaac atcatcgccg gcatcggcgt
     2101 cggcttcgag tcgacggcga tcccgatcct ccttatcgcc gccgcgatct tcgtcgccaa
     2161 ctacttcgcc ggcctgtacg gcgtcgccct cgcggcgctc ggcatgctgt cgaccaccgg
     2221 catccagctc gccgtcgacg cttacggtcc catttcggac aacgccggtg gcatcgccga
     2281 gatgagcggc ctcccgcacg aggtccgcga gcgcaccgac aacctcgacg cggtcggcaa
     2341 cacgaccgcc gcgatcggca agggcttcgc catcgcctcg gccgccctcg ccgccctgtc
     2401 gctgttcgcg gcctaccgca acatcgccct caaggacagc gacctgctgc tcaccgaccc
     2461 caaggtcctc atcggcctgc tgatcggcgg catgctcccg ttcctgttct cggcgatggc
     2521 catgaaggcc gtcggcgccg ccgcgatgga catgatcacc gaggtccgcc gccagttccg
     2581 ggagatcccc gtgctgcgcg aggccctgac gctcgtcgcc cgcgccgaga aggaaggccg
     2641 cgacctcacc tccgaggagg aggcccaggt cctcgccgcc ggtgagaaga ccgaggtcga
     2701 gcgctgcgtc gccatctcga ccaaggcctc gatccgccgc atggtcatgc cgggcgtgct
     2761 cgccctgacc tcgcccgtca tcgtcggctt ctggtcgcgc aacgcgctcg gcggcctgct
     2821 cgccggcacc ctggtcaccg gcgtcatgct ggcgatcttc atgtccaacg ccggtggcgc
     2881 gtgggacaac gccaagaagc aggtcgagga ccagaagaag gactccagcc aggccgtcaa
     2941 caccggcaag ggctcggagc gccacaaggc cgccgtcatc ggcgacaccg tcggcgaccc
     3001 cttcaaggac accgcgggcc ccagcctcaa catcctcatc aagctgatga cgatcgtcgc
     3061 tgtcgtcatc gccccgatcc tcgtcaagta cggcggcctg taaaccccag gctctcgccc
     3121 gtcgacccgc gtgctggatc cagcacgcgg gttttgcttt cggtgggcct cctctgtcgg
     3181 ggctcgactt cccgagtggc gcgctcggct tcacaagtgg cgagctcgga gagcgtgcgg
     3241 gctcgacttc ccgagtggcg agctcggaga gcttgcgggc tcgacttccc gatggcgagc
     3301 tcggagaccg cggggcgagg cttcatgggc cggggagggg ccgacaggct gccctactcc
     3361 ccgggccagc accgtatgcg ccctgctcgt tgcgagcatc actgacgacg ctcggccgac
     3421 cacccgatcg tgccggtctc gtgagtcccg cggcgtcgcg aggcgtcgcg gccgccagag
     3481 tgagatgcgc cgactcgacg ttgccccggc gccgacaagc ggggcgcaga cagcgtccgt
     3541 ccgggggtag ggccgccgag gcgggggaag cagcgggagg acccaggggt cgcgcgcaga
     3601 cgccccctcg ttccttcgct cacgagaggc atggtcggtg aaaccgtcgc ggtgatcccg
     3661 acagatgggg cggcgagcac gtcccctggg aggacccgcg tcgcagggac ccgcagcccg
     3721 gcggcccccc cggcccaggc agcctcgccc cgcgttcttc gatctcgcca cgcccgggcc
     3781 gccgcgaagc cagagccggc gaggcccccg gccacttg
//
LOCUS       DASWNJ010001363         3587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_94383, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001363 DASWNJ010000000
VERSION     DASWNJ010001363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94383"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..296
                     /locus_tag="VGB85_22725"
     CDS             36..296
                     /locus_tag="VGB85_22725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136922.1"
                     /translation="MFDTINAAFMIVALILAVIAGLWFGAILGESTNATDKDGKPTKR
                     RNFTQALRNAATTSAVRLWKWNRARTREREQAERRRGEPRDL"
     gene            complement(330..3062)
                     /locus_tag="VGB85_22730"
     CDS             complement(330..3062)
                     /locus_tag="VGB85_22730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136923.1"
                     /translation="MSCLFAMLAPAAASSDDPIPNLDALALAPTVGWLMLTGFALLVA
                     IFVIHRETWRRLFLRAEDPRSVAAFRIAFGLMTMANINGLWELFHYLFTDEGLFFADV
                     AQQVFAKEQFEGFADGIGEGEPYGFLGWAGFLEFIKGPKFSLLFFWDTPTAFWLHLVA
                     FWVACTCLIVGYKTKYAKWATFILFNSIILRNQVFWEGTENVYRCFLFYLCLSRCGAA
                     YSVDNWLRCRRLRKQGLLSERDGPGGGAGVAPSPEHPRGLAAVYELVPAWPRMLMILQ
                     VAALYCSTGTVKNGTIWMNGDAFYYALNLDHFYRFEPQQLSAIFGTNLFRLNTWVVHW
                     WEACFPLVVLGLVSRFMRREKVPPPPRIQLWVARGMWVLLGLVACAVVQVTLPVHIPA
                     RVPEGKWTLADWQRWFPVVWISGMAVIAGAIAWMRLRPPAVTIRGQRYVLDLDWFCTW
                     FLGRRVWLGLGMIFHAHLIMMMNIGWFTPATLSTYFVFLNGNELAHLLQPLGAGLARL
                     GIPMPASVRRGDPPLPAQDPTLPQLHRDAAAMPAWALFAGLGVAIGGVWLALPREVVN
                     ASGAVTTRPGMHWALVGVGIFAGLAVIGFITAWRHGATQLSRIDPARGTPRRPWAYGP
                     MGRYLACALCVYQITGVAIWLLPEKDSLGGNGQNSWRLRAQDPFKWWLRITQTNQGWK
                     MFAPNPPRTNLMMRVLVTDANGEVYDLNTDVYHPANKPIPWIFYSRIRKINRRIVGAE
                     GGKGKWYQKWHGRYICRDWALHHDGVPPKQVDLIKVSYQIPTPEEVAEKGPYVPEERM
                     QRTHSTRHVHTTHCDDINAQLPNHIRERHGLPPLPADTPFRPWVKNRREAFDKKVAKL
                     AKQGRDPNRVPFVPLLGGLVVLVAGWRWWQLDRDNKRRALAQELAAPVPETPVS"
BASE COUNT          610 a         1212 c         1250 g          515 t
ORIGIN      
        1 ccgagcgcag tccagttcgc tgagcccgag gcaagatgtt cgacaccatc aacgcggcct
       61 tcatgatcgt cgccctcatc ctcgcggtga tcgccggcct gtggttcggc gcgatcctcg
      121 gtgagagcac caacgctacc gacaaggacg gcaagccgac caagcgccgc aacttcacgc
      181 aggcgctgcg taacgccgcc accacctcgg ccgtccgcct gtggaagtgg aaccgcgccc
      241 gcacccgtga gcgcgagcag gccgagcgcc gccgcggcga gccgcgggac ctctgacgcc
      301 cgcgggacct ctgaactggc cgcggcgccc taactgaccg gtgtctccgg caccggggca
      361 gcgagctcct gggcgagcgc gcggcgcttg ttgtcgcggt cgagctgcca ccagcgccag
      421 ccggcgacga gcacgacgag cccgccgagc agcggcacga acggcacccg gttggggtcg
      481 cggccctgct tggcgagctt ggcgaccttc ttatcaaaag cctcgcggcg gttcttgacc
      541 cacggacgaa acggcgtgtc ggcgggcagc ggcgggaggc cgtggcgctc gcggatgtgg
      601 ttgggcagct gcgcgttgat gtcgtcgcag tgggtcgtgt gcacgtgccg cgtgctgtgg
      661 gtcctctgca tgcgctcctc gggcacgtag ggacccttct ccgcgacctc ctcgggcgtc
      721 gggatctggt acgacacctt aattaaatcg acctgcttgg gcggcacgcc gtcgtggtgg
      781 agggcccagt cgcggcagat gtagcgcccg tgccacttct ggtaccactt gcccttgcca
      841 ccctcggcgc cgacgatcct gcggttgatc ttgcggatcc gcgagtagaa gatccacggg
      901 atcggcttgt tggcggggtg gtacacgtcc gtgttgaggt cgtagacctc gccgttggcg
      961 tcggtcacca gcacgcgcat catcaggttg gtgcgcggcg ggttgggggc gaacatcttc
     1021 caaccctggt tggtctgggt gatccgcagc caccacttga aggggtcctg cgcgcgcagg
     1081 cgccaggagt tctggccgtt gccgccgagc gagtccttct ccggcaacag ccagatcgcc
     1141 acgccggtga tctgatagac gcacagagcg caggcgaggt agcgccccat cggcccgtac
     1201 gcccacggcc tccgtggggt cccgcgcgcc gggtcgatgc gcgacagctg cgtcgccccg
     1261 tgccgccacg cggtgataaa gccgatcacc gccaggccgg cgaagatgcc gacgccgacg
     1321 agggcccagt gcatgccggg gcgcgtcgtc acggcgccgg aggcgttgac gacctcgcgc
     1381 ggcaaggcga gccacacccc gccgatagcg acgccgagcc cggcgaacag cgcccaggcg
     1441 ggcatcgccg cggcgtcgcg gtgcagctgc ggcagcgtcg ggtcctgggc gggcagcggc
     1501 gggtctccgc ggcgcacgct ggccggcatc gggatgccga gacgggccag gccggcgccg
     1561 agcggctgca gcaggtgcgc gagctcgttg ccgttgagga acacgaagta ggtcgacagc
     1621 gtcgccggcg tgaaccaccc gatgttcatc atcatgatca ggtgggcgtg gaagatcatg
     1681 ccgaggccga gccacacgcg ccggccgagg aaccaggtgc agaaccagtc gaggtcgagc
     1741 acgtaccgct ggccgcggat cgtgaccgcg ggcggccgca ggcgcatcca cgcgatcgcg
     1801 ccggcgatga cggccatgcc ggagatccac acgaccggga accaccgctg ccagtcggcg
     1861 agcgtccact tgccctcggg gacccgcgcc gggatgtgca ccggcagcgt gacctggacg
     1921 accgcgcagg cgaccaggcc gagcagcacc cacatgccgc gcgcgaccca gagctggatg
     1981 cgtggcggcg gggggacctt ctcgcggcgc atgaaccggc tgacgagccc gagcacgacg
     2041 agcgggaagc aggcctccca ccagtgcacg acccaggtat tgaggcgaaa caggttggtg
     2101 ccgaagatcg ccgacagctg ctggggctcg aagcgataga agtggtcgag gttgagcgcg
     2161 tagtagaacg cgtccccgtt catccagatc gtgccgttct tgacggtgcc ggtcgagcag
     2221 tacagcgccg cgacctgcag gatcatcagc atgcgcggcc acgcggggac cagctcgtag
     2281 acggccgcga ggccgcgcgg gtgctcgggg ctcggggcca cgccagcgcc gccgccgggc
     2341 ccgtcgcgct cgctcagcag cccttgcttg cgcagccgcc ggcaacgcaa ccagttgtcg
     2401 accgagtagg ccgcgccgca gcgcgacagg cacaggtaga acaggaagca gcgatagacg
     2461 ttctccgtgc cctcccagaa cacctgattg cgcaggatga tcgagttgaa caagatgaag
     2521 gtcgcccact tggcatactt cgtcttgtag ccgacgatca ggcaggtaca cgcgacccaa
     2581 aaggccacca ggtgcagcca gaaggccgtc ggcgtgtccc agaagaacag cagcgagaac
     2641 ttcggacctt tgatgaattc gaggaagccg gcccacccga ggaagccgta cggctcaccc
     2701 tcgccgatgc cgtccgcgaa gccctcgaac tgctccttgg cgaacacctg ctgcgcgacg
     2761 tcggcgaaga acaggccctc gtcggtgaac agataatgaa acagctccca caggccgttg
     2821 atgttggcca tcgtcatcag cccgaaggcg atgcgaaacg cggccaccga gcgcgggtcc
     2881 tcggcgcgca ggaacagccg ccgccaggtc tcgcggtgaa tgacgaagat cgccaccaac
     2941 aacgcgaagc cggtcaacat caaccacccg accgtcggcg ccagcgccag cgcatcgagg
     3001 ttggggatcg ggtcgtccga agacgcagcc gcaggtgcca gcatggcaaa caggcaggac
     3061 atggtggcga tcattgcgat cctcttgcgc tctctggctc gtcggctgct cgggtggtca
     3121 taaaatgtgg atcgattgcc cgctcgcgcg tgcactcgct cgcggtgatc gactgccagc
     3181 tcgcgtgcgc gagtgtgcgg agctcgaccg cctgctcgcg tgaacgagag gtgcggagct
     3241 cggctgcctg ctcgcgtcgg cgagtgacgc gaagctcggc tgcctgctca cgtggaccag
     3301 agacgcgcag ctcgactgcc tgctcgcgtg aacgagtgac gcgaagctcg actgcctgct
     3361 cacgtggacc agagacgcga agctcgactg cctgctcgcg tgaacgagtg acgcgaagct
     3421 cgactgcctg ctcgcgtgga ccagagacgc ggggcgaggc tgcctgggcc ggggaggggc
     3481 cggtggactg cgggtccctg cgacgcgggt cctcccgggt ctcgtgctcg ccgccccatc
     3541 tggcccgaac gacatccgct gtcaccgacc ctggatcgca cgcccgc
//
LOCUS       DASWNJ010001364         4244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_307743, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001364 DASWNJ010000000
VERSION     DASWNJ010001364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307743"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1041
                     /locus_tag="VGB85_22735"
     CDS             223..1041
                     /locus_tag="VGB85_22735"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEY0136924.1"
                     /translation="MKILVTVKRIPDPDESLKFAAGGLDYSASKWVPNAFDEYAVETA
                     LRLAEVVGGTQRLAEVIVLSICPENQRQHVTQFLAMGADRGIIVDADPDKLDTITVSK
                     LIAAVFKKEACDLLISGKLSQDNEGNQVPQRVAGLLALPQACFAAGIKWDQTGRALDV
                     SREVDDGVEQKRIPLPAVVSVDLRIVLPKSVANGSTPATHAYNEGPRLASLKGITMAK
                     RKPVAVHKLADLGVTATGTEQNVSVAAPPKRKAGQKVKTVEELFEKLTKEAKVL"
     gene            1038..2024
                     /locus_tag="VGB85_22740"
     CDS             1038..2024
                     /locus_tag="VGB85_22740"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEY0136925.1"
                     /translation="MSKVLVILETANGKIRPHCLPGITAGARISAATGKPLQLLVLGA
                     DASAAETIAAAGYGATAVLSMAAPGLEPFTAEAWADAIVAAVKAVGGDGLNGLIIGGS
                     ASSTLRDALPRAAALLDAPLVADVVGVKDATTFTRPVSAGRALVDVKVSAPIVFFTAR
                     GTEFEPAQPGAGVSVSTLSAPQVNNRGVQVKGVQKTESSRPALTEAKVVVSGGRGMRE
                     GANFKLLEQLTDLLGGALGASRAATDAGMVPADLQVGQTGKIVAPDLYIAVAVSGAIQ
                     HLAGMKGSKVIVAINKDEEAPLFQVADYALAAKWEDAIPRLIELIKAKRGAA"
     gene            2172..3785
                     /locus_tag="VGB85_22745"
     CDS             2172..3785
                     /locus_tag="VGB85_22745"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0136926.1"
                     /translation="MPGSVASRRERVWALGIALLACIAVLACSQTMLAWMTGAQVLRL
                     GPAAAVLAGISGLAHLLLLYMSSRTGRIDQAGEAAVRALIFAPPLALLVVVPSMWGPA
                     LFATSVPNLLPQVVPVLAAVWSAALLASAPVGVLLRRLAPIDQAEPDSVVGRWANLFL
                     ACTTATALIALGYALGRGGADPPPTGLRELGLIVGLFGVVVLAVLAGLGVGAAGSGEV
                     VAVARRLDRVDEYTGAKPLVVTRLDSMGELMSELESLRRDLAAEHQRYRDTLAQTREA
                     AVAKAEFLAAVSHELRTPLNSICGFSQLLLEGMMPTILAAEQREDVRLIRTSGMQLLG
                     LINDILDISMIESGELRLYFAAEDIAEIVDEVARIHRPLVQEAGLELRAEVPEGLPRV
                     VCDRRRISQILNNLLSNATKFTERGSITISALHDPLAGRLLVRVTDTGIGIAPADIDA
                     IFEEYHQVGSLSSRKQGTGLGLAIARSIAVHHGGALSVSSTPGRGSSFTLALPLDPPR
                     KPTSIDMTAEVVRASQRFRHVRHAPEQAS"
     gene            3782..>4244
                     /locus_tag="VGB85_22750"
     CDS             3782..>4244
                     /locus_tag="VGB85_22750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136927.1"
                     /translation="MNADSESSGNLSESSGWDGRTDELDVESDLVPGLRARGLPTRLA
                     AERDVMAVLAAAFVAGATMTVTTTLITWDGRSADAPWPVFALVVPHLLVLAAVLAAAY
                     LILPAARWLAPEDLARRRRPPLLRLAWILGAPAWPIGRLLVARERRELGRVL"
BASE COUNT          595 a         1695 c         1365 g          589 t
ORIGIN      
        1 ggccgcgggc aacgctaacg cacgccccgg cgggccgcaa gaccggcgag gcgacccgcg
       61 acgcggtcag gcccgccgcc cccgccgcca gcaccgcggc ggagccccga acgccggcgc
      121 cggggcggcc ccgcggacgg gcgtccgagg tctgcgccgc agcgacgcat tgacaggtcc
      181 agggtcgcgg gtggtacacc ccccgctcag gaggagtccc ttttgaagat cctggttact
      241 gtcaagcgga tccccgatcc ggacgagagc ctcaagttcg ccgcgggcgg gctcgactac
      301 tcggcctcga agtgggtccc caacgcgttc gatgagtacg ccgtggagac cgccctgcgc
      361 ctcgcggagg tcgtcggggg cacccagcgg ctcgccgagg tcatcgtgct cagcatctgc
      421 ccggagaacc agcgtcagca cgtgacgcag ttcctggcga tgggcgccga ccgcggcatc
      481 atcgtcgacg ccgacccgga caagctcgac acgatcaccg tctcgaagct gatcgccgcc
      541 gtcttcaaga aggaagcgtg cgacctgctg atctcgggca agctgtcgca ggacaacgag
      601 ggtaaccagg tgccgcagcg cgtcgccggc ctcctcgccc tgccccaggc ctgcttcgcc
      661 gccggcatca agtgggacca gaccggtcgc gccctcgatg tctcacgcga agtcgacgac
      721 ggcgtcgagc aaaagcgcat cccgctgcca gccgtcgtct ccgtcgacct gcgcatcgtc
      781 ctccccaaga gcgtcgccaa cggctcgacc ccggcgacgc acgcctacaa cgagggcccg
      841 cgcctcgcct cgctcaaggg catcacgatg gccaagcgca agccggtcgc cgtgcacaag
      901 ctcgccgacc tcggcgtgac cgcaaccggc accgagcaga acgtctccgt cgccgcgccg
      961 ccgaagcgca aggccggcca gaaggtcaag accgtcgagg agctgttcga gaagctgacg
     1021 aaggaggcga aggttctatg agcaaggtac tcgtcatcct cgagaccgcg aacggcaaga
     1081 tccgtccgca ctgtctccct ggcatcaccg ccggcgcgcg gatctccgcc gccaccggca
     1141 agccgctgca gctgctcgtg ctcggcgcgg atgcgtccgc cgccgagacg atcgcagccg
     1201 ccggctacgg cgccaccgcc gtgctgtcga tggccgcccc cggcctcgag cccttcaccg
     1261 ccgaagcctg ggccgacgcg atcgtcgccg ccgtcaaggc cgtcggcggt gacggcctga
     1321 atggcctgat catcggcggc agcgcctcct cgaccctgcg tgacgcgctc ccacgcgccg
     1381 cggcgttgtt ggacgccccg ctcgtcgccg acgtcgtcgg ggtcaaggac gcgaccacct
     1441 tcacccgccc ggtctcggcc ggtcgcgccc tcgtcgacgt caaggtcagc gcaccgatcg
     1501 tgttcttcac ggcgcgcggc accgagttcg agccggccca gccgggcgcg ggcgtgagcg
     1561 tcagcaccct ctcggcgccc caggtcaaca accgcggcgt gcaggtcaag ggcgtccaga
     1621 agaccgagag cagccgcccc gcgctcaccg aggccaaggt cgtcgtctcc ggcggccgcg
     1681 gcatgcgcga gggcgccaac ttcaagctgc tcgagcagct gaccgacctg ctgggcggcg
     1741 ccctcggggc gagccgcgcg gcgaccgacg ccggcatggt cccggccgac ctgcaggtcg
     1801 gacagaccgg caagatcgtc gcgccggacc tgtatatcgc cgtcgccgtc agcggcgcga
     1861 tccagcacct cgcgggcatg aagggctcga aggtcatcgt cgcgatcaac aaggacgagg
     1921 aggcccccct gttccaggtc gccgactacg ccctcgccgc caagtgggag gatgcgatcc
     1981 cccgcctcat cgagctgatc aaggccaagc gcggcgcggc ctgacgcccc cgcttccaca
     2041 tcgcgtggag cgcgatcgtt ccaacttgaa agagggtcgt cgagcaaaaa tcgacgaccc
     2101 tcttctcacg tcatgacacg ctcccggcac ctgctcgtca caggcgcggc gccggtgata
     2161 tcctcgcctt cgtgccggga tcggtggctt cgcggcgaga acgcgtgtgg gcgctgggca
     2221 tcgccctgct cgcgtgcatc gccgtgctcg cctgctccca gacgatgctc gcctggatga
     2281 ccggcgccca ggtcctgcgc ctcggacccg ccgccgccgt gctcgccggc atctccggac
     2341 tcgcccacct cctgctcctc tacatgtcct cacggacagg tcgcatcgac caggccggcg
     2401 aggccgccgt gcgcgcgctg atcttcgccc cgccgctggc cctgctcgtc gttgtcccgt
     2461 cgatgtgggg ccccgccctg ttcgccacca gcgtgccgaa cctgctgccc caggtcgtgc
     2521 ccgtgctcgc cgccgtgtgg tcggccgccc tgctcgcctc ggccccggtc ggcgtgctgc
     2581 tgcgccggct cgcgccgatc gaccaggccg agcccgactc cgtcgtcggg cgctgggcca
     2641 acctgttcct cgcctgcacc accgccaccg ccctgatcgc gctcggctac gcgctcggcc
     2701 gcggcggcgc cgacccgccg cccaccggcc tgcgcgagct cgggttgatt gttggcctgt
     2761 tcggcgtcgt cgtgctcgcc gtgctcgccg gcctcggcgt cggcgcggcc ggcagcgggg
     2821 aggtcgtcgc cgtcgcccgc cgactcgacc gcgtcgacga gtacaccggc gccaagccgc
     2881 tcgtcgtcac ccgcctcgac agcatgggcg agctgatgag cgagctcgag tccctgcgtc
     2941 gcgacctcgc cgccgagcac cagcgctacc gcgacaccct cgcgcagacc cgcgaggccg
     3001 cggtcgccaa ggccgagttc ctcgccgccg tcagccacga gctacgcacc ccgctcaact
     3061 cgatctgcgg cttctcgcag ctgctgctcg aaggaatgat gccgacgatc ctcgccgccg
     3121 agcagcgcga ggacgtgcgc ctgatccgga ccagtggcat gcagctgctc ggcctgatca
     3181 acgacatcct cgacatctcg atgatcgagt cgggagaact tcgcctgtac ttcgccgccg
     3241 aggacatcgc cgagatcgtc gacgaggtcg cccggatcca ccgcccgctg gtccaggagg
     3301 ccggcctcga gctgcgcgcc gaggtccccg agggcctccc gcgcgtcgtc tgcgaccgcc
     3361 gccggatctc ccagatcctc aacaacttgc tgtcgaacgc gaccaagttc accgagcgcg
     3421 gcagcatcac gatctcggcg ctgcacgacc cgctcgccgg ccgcctgctc gtccgcgtca
     3481 ccgacaccgg catcggcatc gctcccgccg acatcgacgc gatcttcgag gagtaccacc
     3541 aggtcggcag cctctcgagc cgcaagcagg gcaccggcct cggcctggcg atcgcccgca
     3601 gcatcgccgt ccaccacggc ggcgcgctgt ccgtcagctc cacccccggc cgcggctcga
     3661 gcttcaccct cgccctcccc ctcgaccccc cgcgcaagcc gacctcgatc gacatgaccg
     3721 ccgaggtcgt ccgcgcctcg cagcgctttc gccacgtccg ccacgccccg gagcaagcct
     3781 cgtgaacgcc gactccgaga gcagcggcaa cctgtccgaa agttcaggct gggacggccg
     3841 gaccgacgag ctcgacgtcg agtccgacct ggtccccggc ctgcgcgccc gcggcctgcc
     3901 gacccgcctc gccgccgagc gcgacgtcat ggccgtgctc gccgccgcct tcgtcgccgg
     3961 cgcgacgatg accgtcacca ccaccctgat cacctgggac ggccgcagcg ccgacgcgcc
     4021 gtggccggtg ttcgcgctgg tcgtcccgca cctgctcgtg ctcgccgcgg tgctcgccgc
     4081 cgcctacctg atcctgcccg ccgcccgctg gctcgccccc gaggacctcg cccgccgccg
     4141 ccgcccgccg ctgctgcgcc tcgcgtggat cctcggcgcg cccgcgtggc cgatcggtcg
     4201 cctgctggtc gcacgtgagc gccgcgagct cggccgcgtg ctcg
//
LOCUS       DASWNJ010001365         3596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_129085, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001365 DASWNJ010000000
VERSION     DASWNJ010001365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129085"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VGB85_22755"
     CDS             complement(<1..371)
                     /locus_tag="VGB85_22755"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEY0136928.1"
                     /translation="MAGQIWLLRGDITGRDVDAIVNAANSSLLGGGGVDGAIHRAAGP
                     GLLEECRPLRGCPTGQAKITGGHRLQARHVIHTVGPVWQGGSKGEPAALASCYRNSLA
                     LAAKHGLRSVAFPAISCGVYG"
     gene            448..1131
                     /locus_tag="VGB85_22760"
     CDS             448..1131
                     /locus_tag="VGB85_22760"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0136929.1"
                     /translation="MPPRSELPHEPEADPRARRLARKSRRQENRRHIVLACARDVLVA
                     HGPVAFTMEQLAAAAGTSKATLYYYFRSREEVIGELAVAVLRREVEVLSRVVIAAETG
                     IDGLAALVRARVEHHLADPDGFRILYVWAPVLGDPQRLLMSEIYPLSAVVNTTLEAKL
                     QRDRKAGLLDPDADPRRLADLAWLTAQGLLGLAMNPPTTDPPFSWQALCDEACNTLVR
                     AAKRSRPPT"
     gene            1226..2011
                     /locus_tag="VGB85_22765"
     CDS             1226..2011
                     /locus_tag="VGB85_22765"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY0136930.1"
                     /translation="MFEHYLHHQVPDTRRRWKLALALNVAGFATAGLVGFTWLMDKMM
                     IAQVAPPTGHFVMVQMSLDSLPPPPPAPPAAPAVKAQDDVKPEPDEDPLTPPEDFTET
                     LPTPRPRVAKVTGTGAPDSTGTLPIGGPSKFGLPGGFPGMPPITGIAVRQPSKPPETR
                     TPVPIATVHAQAIYAPPPDQGKLGATKAATFDRRPGENETAFCVDPTGRTTDVRTVKK
                     FPNDPRVDEICRDTIKTWRFKPFLVDGKPTRTCSVQVFNISFK"
     gene            complement(2015..3139)
                     /locus_tag="VGB85_22770"
     CDS             complement(2015..3139)
                     /locus_tag="VGB85_22770"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY0136931.1"
                     /translation="MRATLNLCSLVCLLGFPGCGDNGGGTETGSDSSGTSSSGGVVDT
                     SSTGDETPTGEPATTGPVTSTGETTGTQPGTTSTDEGTTGGESTTGDETTGGAIDCDA
                     VGAGPFTLEVFVSGFTGSEDLAFDGKGGLALKRGADIVVVRADKSETTLAMAIPPAYG
                     TRFLADGRLLVALPQGGAVITVDETDVKAKFLGGLKGPNGIYPDLEGNVWVTEFGGSR
                     LIRVAADMSETTIVSGAAASSANGVVYDPLRGFVFYTNYQSGQIRRVAIDGEGTPGSP
                     ALVTTIADAAPDGLTLDACGNLYVVDQGNSVLYRVMLDELGQATAPAIALASFPSNVA
                     NAQFGVGDGFDARTLYLAGNPGDVYALALEVPGAPIVTVQ"
     gene            complement(3150..>3596)
                     /locus_tag="VGB85_22775"
     CDS             complement(3150..>3596)
                     /locus_tag="VGB85_22775"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY0136932.1"
                     /translation="RVQHVPSVRRWAPRAPIKRVAWGLLVLFMVGMGTLHFTATPFFL
                     RAMPPWLPLHLEAVYLSGVCEIAGGLGLLIPRLRVAAAWGLVALFVAVFPANVYVAVA
                     GVQLTPEPIAWWIWWARLPLQAVFIAWAWWMTRDDGEDVADATRLA"
BASE COUNT          546 a         1422 c         1110 g          518 t
ORIGIN      
        1 tagccgtaca ctccgcagga gatcgcagga aacgccaccg accgcagccc gtgcttcgcg
       61 gccagcgcga ggctgttgcg atagcagctc gccagcgccg ccggctcgcc cttcgacccg
      121 ccttgccaca ccggcccgac cgtgtggatc acgtgtcgcg cctgcaggcg atgcccgccg
      181 gtgatcttcg cctgtcccgt gggacagcca cgcagcggcc ggcactcctc gagcagcccc
      241 ggtccggccg cgcgatggat cgcaccatcg accccgccgc cgccgagcag gctgctgttc
      301 gccgcgttga cgatcgcgtc gacgtcacgg cccgtgatgt cgccccggag cagccagatc
      361 tgtcctgcca tgccccagca tcgcacgcca ttgcattttt ttcgacgcca ggtcagcttc
      421 gccccggacc cgcgtttccc tttggccatg ccgccccgca gcgaactccc gcacgagccc
      481 gaggctgacc cgagggccag acgactcgcg cgtaaatcga ggcgccagga gaaccgccgg
      541 cacatcgtcc tcgcgtgcgc gcgcgacgtc ctggtcgccc acggacccgt cgccttcacg
      601 atggagcagc tcgccgccgc cgcgggcacc agcaaggcca cgctttatta ttactttcgt
      661 tcgcgcgagg aggtcatcgg cgagctcgcc gtcgccgtgc tgcgccggga ggtcgaggtg
      721 ctcagtcgcg tcgtcatcgc cgccgagacc ggcatcgatg gcctcgccgc gctcgtgcgc
      781 gcccgcgtcg agcaccacct cgccgacccc gacggctttc gcatcctcta cgtgtgggcg
      841 cccgtgctcg gcgaccccca gcgcctgttg atgtccgaga tttatccgct gagcgccgtc
      901 gtcaacacca cgctcgaggc caagctgcag cgcgaccgca aggccggcct gctcgacccg
      961 gacgccgacc cgcgccggct cgccgacctc gcgtggttga ccgcgcaggg tctgctcgga
     1021 ctggcgatga acccgccgac caccgacccg ccgttctcgt ggcaggcgct ctgcgatgag
     1081 gcctgcaaca ccctggttcg ggccgcaaag cgctcgcgac cgcccacctg acccctcccg
     1141 gatcagacat ttataagaaa actaatccgt atcaaatcga aacttcctac cgtttgtttg
     1201 gtcatccaac cccggaggaa gtttcatgtt cgagcactac ctacaccacc aggtccctga
     1261 cactcgtcgc cgctggaagc tggccctcgc gctcaacgtc gccggcttcg ccacggctgg
     1321 cctcgtcggc ttcacctggc tgatggacaa gatgatgatc gcccaggtcg ccccgccgac
     1381 cggccacttc gtgatggtcc agatgtcgct ggactcgctg ccaccgccgc cgccggcccc
     1441 tccggccgcg ccggcggtca aggcccagga cgacgtcaag cccgagcccg acgaggaccc
     1501 gctgaccccg ccggaggact tcaccgagac gctgccgacc ccgcggccgc gcgtcgcaaa
     1561 ggtcaccggc accggcgcgc ccgacagcac cggcacgctc ccgatcggcg gcccgtcgaa
     1621 gttcggcctc cccgggggct tccccggcat gccgccgatc accggcatcg ccgtgcgcca
     1681 gccgagcaaa cccccggaga cgcgcacccc cgtgccgatc gccaccgtgc acgcccaggc
     1741 gatctacgcc ccgccgccgg atcagggcaa gctcggcgcg acgaaggccg ccaccttcga
     1801 ccgccgcccc ggcgagaacg agaccgcctt ctgtgtcgac ccgaccgggc gcaccaccga
     1861 cgtgcgcacc gtcaagaagt tccccaacga ccccagggtc gacgagatct gtcgcgacac
     1921 gatcaagacc tggcgcttca agccgttcct cgtcgacggc aagccgaccc ggacctgcag
     1981 cgtccaggtc ttcaacatct cgttcaagtg accctcactg caccgtgacg atcggggccc
     2041 cggggacctc gagcgccagc gcgtacacgt cgccggggtt gcccgcgagg tacagcgtgc
     2101 gcgcatcgaa gccgtcgccg acgccgaact gcgcgttggc gacgttgctc gggaagctcg
     2161 ccagcgcgat cgccggcgcg gtcgcctggc cgagctcgtc gagcatcacg cggtacagca
     2221 cgctgttgcc ctggtcgacc acgtacaggt tgccgcacgc gtcgagggtc agcccgtcgg
     2281 gcgccgcatc ggcgatcgtc gtcaccagcg cggggctgcc gggcgtgccc tcgccgtcga
     2341 tcgccacccg gcggatctgc ccgctctgat agttcgtgta gaacacgaag ccgcgcagcg
     2401 ggtcgtacac caccccgttg gcgctgctcg ccgcggcgcc ggacacgatc gtcgtctcgc
     2461 tcatgtcggc cgcgacgcgg atcaggcggc tgccgccgaa ctccgtcacc cacacgttgc
     2521 cctcgaggtc cgggtagatc ccgttcggac ccttgagccc gccgaggaac ttcgccttca
     2581 cgtccgtctc gtcgaccgtg atcacggccc cgccctgcgg cagcgcgacc agcaggcgcc
     2641 cgtccgccag gaagcgcgtg ccgtaggccg gcgggatcgc catcgccagc gtcgtctcgc
     2701 tcttgtcggc gcgcacgacc acgatgtcgg cgccgcgctt gagcgccagc ccgcccttgc
     2761 cgtcgaaggc gaggtcctcg ctgcccgtga acccgctgac gaacacctcg agcgtgaacg
     2821 gccccgcccc gaccgcgtca cagtcgatcg ccccgccggt cgtctcgtcg ccggtcgtcg
     2881 actctccgcc ggtcgtgccc tcgtccgtgc tcgtcgtgcc cggctgcgta cccgtcgtct
     2941 cgcccgtgct cgtgaccggg ccggtcgtcg ccggctcgcc tgtcggagtc tcgtcgccgg
     3001 tgctgctcgt gtcgaccacg ccgcccgagc tgctcgtacc ggagctgtcg ctgcccgtct
     3061 ccgtgccgcc gccgttgtca ccgcagcccg ggaacccgag caagcacacc agggagcaca
     3121 ggttcaaggt cgcgcgcatg gcggggaagc tacgccagcc tggttgcatc ggcaacgtct
     3181 tccccatcat cccgcgtcat ccaccacgcc cacgcgatga acaccgcctg caacggcagt
     3241 cgggcccacc agatccacca cgcgatcggc tccggagtca gctgcacgcc cgcgacggcg
     3301 acgtacacgt tggccgggaa cacggcgacg aacagcgcga cgagccccca cgccgccgcg
     3361 acccgcagcc gcgggatcag cagcccgagc ccgccggcga tctcacacac gccgctgagg
     3421 tacacggcct ccaggtgcaa gggcagccac ggcggcatcg cgcgcaggaa gaacggcgtc
     3481 gcggtgaagt gcagcgtccc catgccgacc atgaacagga ccaacagacc ccacgcgacg
     3541 cgcttgatcg gggcccgggg cgcccacctc ctgaccgatg ggacatgttg cacccg
//
LOCUS       DASWNJ010001366         1971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_406849, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001366 DASWNJ010000000
VERSION     DASWNJ010001366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406849"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..683)
                     /locus_tag="VGB85_22780"
     CDS             complement(54..683)
                     /locus_tag="VGB85_22780"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEY0136933.1"
                     /translation="MTASHDLHGACPDSCRVALLIIDMINQLDFAEAEQLRPQAEAAA
                     TRIAALAERARAAGVPVIYVNDNFGRWRSDFKATLEQVVRDTPGRAIAEILRPHETDY
                     FVLKPKHSGFFATALDVLLRYLDARWLILTGISGDVCVLFTAHDAYMRDYGLVVPEDC
                     IASVDPRANEHALEMMRATLGAESTPSDQLELGRYLDPGHRSPREQGES"
     gene            829..>1971
                     /locus_tag="VGB85_22785"
     CDS             829..>1971
                     /locus_tag="VGB85_22785"
                     /inference="COORDINATES: protein motif:HMM:NF023662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136934.1"
                     /translation="MQLAVPRFQFRFVPALGNVIHPGNFHPVGAQAGAEALIDRVLAA
                     TPERPATGSGGNWLLVGGSGGFGSAARAVLGAGHGANTLNLSFDAGPQPESSNKQRKI
                     GSPGFHRSLAIERRLRAIGLVARSLSGDAFDPKMREQAIAELREHFGGQLHGLVWALA
                     APRALDPRTGKAVQSVLKPLGKAADIVTFTSPSESPEGLPKIAKFTLPPGNPEEAIAT
                     QYVMGGRIVEQWVDALLAADLLAPGFTLVTVSYRGNPLNAPVYREGLIGLAKADLEFH
                     TKALNELLRARVGGRGVVVEGPAVVTEASGGIPGVPHYMAQLLDVMGDRFEDPLASML
                     RMFRTKLPLGTGEPELDEQGLLRMDDRELAGEIQDELARRFAGLQVG"
BASE COUNT          268 a          678 c          729 g          296 t
ORIGIN      
        1 ctcctggacc gacacttcga tcgcgggcgc ggggctcggc tcgcccggct ggctcatgac
       61 tcgccctgct cgcgcggcga cctgtgcccg gggtcgaggt agcgcccgag ctcgagctgg
      121 tcggagggcg tgctctcggc gcccagcgtc gcgcgcatca tctccagcgc gtgctcgttg
      181 gctcgcgggt cgacggaggc gatgcagtct tcgggcacga cgagcccgta gtctcgcatg
      241 tatgcgtcgt gcgcggtgaa cagcacgcag acgtcgccgc tgatcccggt gaggatcagc
      301 cagcgcgcgt cgaggtagcg caggagcacg tcgagcgcgg tcgcgaagaa gccggagtgc
      361 ttgggcttga gcacgaagta gtcggtctcg tgcgggcgca ggatctcggc gatcgcgcgg
      421 ccgggcgtgt cgcggacgac ctgctcgagc gtcgccttga agtcggagcg ccagcggccg
      481 aagttgtcgt tgacgtagat gaccgggacc ccggccgcgc gcgcgcgttc ggccagcgcg
      541 gcgatcctcg tcgccgccgc ctcggcctgc gggcgcagct gctcggcctc ggcaaagtcc
      601 agttgattga tcatgtcgat gatcaacaga gcgacgcggc agctgtccgg acatgcgccg
      661 tggaggtcgt ggctggcagt catcccgtgt ctgggatgtg cccggtacat cgccagggca
      721 aggtcgcggg gaggagcccg tcggagggct cacagcggca cgcacgtggc cgtgcgtgcg
      781 catttgcgcg gcaggcgcgt gcgcgcatgt ggtacacggc aaggcgccat gcagctcgcc
      841 gttcctcggt ttcagttccg cttcgtgccc gcgctgggca acgtgatcca cccgggaaat
      901 tttcatccgg tcggggccca ggccggcgcc gaggcgctga tcgaccgcgt gctcgcggcg
      961 acgccggagc ggccggccac gggctcgggc ggtaactggt tgctcgtcgg cggctcgggc
     1021 ggcttcggca gcgccgcgcg tgcggtgctc ggcgcgggcc acggggcgaa caccctcaac
     1081 ctgtccttcg atgccggtcc gcagcccgag agctcgaaca agcagcgcaa gatcggctcg
     1141 cccgggtttc atcgcagcct ggcgatcgag cgccggctgc gggcgatcgg cctcgtcgcc
     1201 cggtcgctgt ccggcgacgc cttcgacccg aagatgcgcg agcaggcgat cgccgagctg
     1261 cgcgagcact tcggcggcca gctgcacggc ctggtgtggg cgctcgcggc cccgcgcgcg
     1321 ctcgaccccc gcaccggcaa ggcggtccag agcgtgctca agccgctcgg caaggccgcc
     1381 gacatcgtca ccttcacgag cccgagcgag agccccgagg gcctgccgaa gatcgccaag
     1441 ttcacgctgc ccccgggcaa ccccgaggag gcgatcgcca cgcagtacgt gatgggcggg
     1501 cgcatcgtcg agcagtgggt cgacgcgctg ctggccgccg acctgctggc gcccggcttc
     1561 accctcgtca ccgtgtcgta ccgcggcaac ccgctcaacg caccggtgta ccgcgagggg
     1621 ctgatcgggc tcgccaaggc cgacctcgag tttcatacca aggccctcaa cgagctgctg
     1681 agggcccgcg tcggcggacg tggggtcgtc gtcgaggggc cggcggtcgt caccgaggcc
     1741 tcgggtggca tcccgggggt gccgcactac atggcgcagc tgctcgatgt catgggcgat
     1801 cgcttcgagg acccgctcgc ctcgatgctg cgcatgttcc ggaccaagct gcctctcggg
     1861 acaggtgagc ccgagctcga cgagcagggc ctgctgcgca tggacgaccg cgagctggcc
     1921 ggcgagatcc aggacgagct ggcgcgccgc ttcgcgggcc tgcaggtcgg c
//
LOCUS       DASWNJ010001367         1680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_406864, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001367 DASWNJ010000000
VERSION     DASWNJ010001367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406864"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..457)
                     /locus_tag="VGB85_22790"
     CDS             complement(155..457)
                     /locus_tag="VGB85_22790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136935.1"
                     /translation="MSQAEAATNLPTLQDLPTKESVRYNTAEARVLGDAWDPESRAVR
                     VAGLTDISDSPEALLFLESKPLGVPIVGVYQGDDGYWILAKDDQDRIVFPRRNLLW"
     gene            721..>1680
                     /locus_tag="VGB85_22795"
     CDS             721..>1680
                     /locus_tag="VGB85_22795"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0136936.1"
                     /translation="MALDVRGRSGEGRLCYIGDVLSYLHIRGLALLDDVALELGRGMN
                     VLTGETGAGKSIIVDALALLRGARGRADLVRQGADSARVEAQFELDLPAQGRIAPVLA
                     ELEAEGGEALVLERVVGRSGRGRSAVQSILTTQGVLERVGEQLIDICSQHEHHSLTHV
                     ARHLDLLDAFAGLDADVVRYGAAYEKWQAAVAAEADLRRRAAEGAARAEFLKYQIEEL
                     ERIDPQPGELPALRARITLLRDGQRWAAFARQAHDALYEADDAIAGRLAALLDEARRG
                     ADSSALLAEIHEQLAAAQVACEEAASAASRFASELSFEPGELEL"
BASE COUNT          220 a          560 c          650 g          250 t
ORIGIN      
        1 agtcgaggag gcggctctgg ttctgccggt cgcgccgcat cctccgcagc gcgtcgttca
       61 tgaatcccgc tgcgctcccg cctcccacgc gacgagcata ctgcggcccg gggggcctgt
      121 cgccgtcacg atctcgcgcc ggggcgcgcg gcggctacca gagcaggttg cggcgcggga
      181 agacgatgcg gtcctggtcg tccttggcga ggatccagta gccgtcgtcg ccctgataca
      241 cgccgacgat cgggacgccg agcggcttgc tctcgaggaa cagcagcgcc tcggggctgt
      301 ccgagatgtc cgtcaggccc gcgacacgca cggcgcggga ctccggatcc caggcatcac
      361 cgaggacgcg cgcctctgcc gtgttgtagc ggacgctttc cttggtcggg aggtcctgca
      421 gcgtcgggag gttggtggca gcttcggctt gggacatgca gcatcacctg aacatggcga
      481 gcacggaggg gtcaagcgcg gcccgggcag tgaacggtgt tcgtgcggag gactcgctgc
      541 gtcattgcca ggatcattgc aggggcatgt cggagaacgc ggtgcacggg tcgcggaggt
      601 ccgcggtttt ccacatcggg ggtcgggcga agttctctct gcgaggccgg ggtaatccgg
      661 acggcgcgcc gggcgaacgg gggtggggag ggggcgtgct tgctctgaac tggatcacgc
      721 gtggctctgg atgttcgcgg tcgctcgggc gaggggcggc tgtgctacat cggcgacgtg
      781 ctctcgtacc tgcacatccg cggactggcc ttgctcgacg acgtcgccct ggagctcggg
      841 cgtggcatga acgtgctcac cggcgagacg ggagcgggca agtcgatcat cgtcgacgcg
      901 ctggcgctgc tgcgtggggc ccgggggcgg gccgacctgg tccgccaggg ggccgactcg
      961 gcccgggtcg aggcgcagtt cgagctcgat ctgccggccc agggccgcat cgccccggtg
     1021 ctcgccgagc tcgaggccga gggcggcgag gcgctggtgc tcgagcgcgt cgtcggtcgc
     1081 tcgggccgcg ggcgctcggc ggtgcagtcg atcctgacga cgcagggcgt gctcgagcgg
     1141 gtcggcgagc agctgatcga catctgcagc cagcacgagc accactcgct gacccacgtc
     1201 gcgcgccacc tcgacctgct cgatgcattc gcggggctcg acgcggacgt cgtccgttac
     1261 ggcgcggcct atgaaaaatg gcaggcggcg gtcgctgccg aggccgacct gcgccggcgc
     1321 gcggccgagg gcgcggcgcg ggcggagttc ctgaagtacc agatcgagga gctcgagcgc
     1381 atcgaccccc agccgggcga gctgccggcg ctgcgcgccc ggatcacgct gctgcgcgac
     1441 ggccagcgct gggcggcgtt cgcccgccag gcccacgatg cgctgtacga ggccgacgac
     1501 gcgatcgccg ggcgactcgc cgcgctgctc gacgaggccc gccgcggcgc cgacagctcg
     1561 gccctgctcg cggagatcca cgagcagctg gcggcggcgc aggtcgcctg cgaggaggcg
     1621 gcctcggcgg cctcgcgctt cgccagtgag ctgtccttcg agccaggtga gctcgagctc
//
LOCUS       DASWNJ010001368         3096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_272949, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001368 DASWNJ010000000
VERSION     DASWNJ010001368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272949"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3096)
                     /locus_tag="VGB85_22800"
     CDS             complement(<1..>3096)
                     /locus_tag="VGB85_22800"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0136937.1"
                     /translation="GLVGRGVDGRADLYALGALLFHAASGRPPFVAADAAELLRLHAV
                     VPAPALASVRPQCSPVLAEVVARLLAKDPDDRYQTGEAVAAALAPLLGLVPEAVPEDR
                     PATALVGRGPELQQLTQAWQQTRRTAERLATGAGALPVIVAMEGEAGAGKSRLVAEFI
                     DRVAGPSAAIVLRSRCSDDGLPFAAVREALDGVALRASADTAVARELAARLRTALAEL
                     AGLLRGLSPALATLVAGPPARGDPKLTREQVDGAVVDGLARLTATGGLVWIVDDLQWI
                     DEGSARLLGRFAARSLEGPALLLCCVRGETGGERIGSGPLPAARGGSGPLPAARGGSG
                     PMPAALGAEPDAPRPRDRARSGPVDVPEGRSWPGSGPLGRRSGPRPAARSRGAWSRFA
                     ELLPASLYLWLGPLPTMAVGQLMAGLLGGRRIPPELVELVARRSDGMPLAVLELVRVM
                     LDEGVLHPSWDVWVADAEALAALPLGGDALRLISGRIAHLSERTGEVLRLAAVCGDRF
                     EPALLARAGAIAAAEVEAAIDEADAAGLIERGRAGYNFVHHRVREALLAALAVEQRQA
                     LHQAVAATLGPREVYARAHHYLAGTLRDTRAVKEACLAAALFALSEAADEEAHRFLSR
                     AQGFAGADEPALVDALAEACLRTGRLVEAEQHIDRGLATAHEPWRRSALLRRRARAAQ
                     LRRDHGRALATMAGAFAALGERLPGHRPIDAVASAWWWLLAVIVGATGVGRGTLRGPA
                     RAHGALVAAYLTEAYVMAYLAFDRRTMVQAVLRHYYVAERLGEGPELAMAYCNYATLL
                     VSLGHRARGLAHARAAEALGQRLGQPTLARVHERLMVVYEFAGEPRVCEAIGRQIHAH
                     ERRWLDTWGQTATGEVLGFNLWLRGRDEAALAALQPLIQELAARGPGVAGQQAQVLLQ
                     VIAGLALTALGRIGEADACFDAAARGFASAPEDRYQNAVLLTCGVMRAAEQGTLGAPL
                     EELIARHAQLELGERRVPFYGRFFYPFVAYARLMQALGSDASVRPAALGRLAQAIAEA
                     E"
BASE COUNT          397 a         1321 c         1080 g          298 t
ORIGIN      
        1 gctcggcctc cgcgatcgcc tgggcgagcc gcccgagcgc cgccgggcgg acgctcgcgt
       61 cgctgccgag cgcctgcatc agccgcgcgt acgcgacgaa gggatagaag aagcggccgt
      121 agaacggcac ccgccgctcg ccgagctcga gctgcgcgtg gcgggcgatc agctcctcga
      181 gcggcgcgcc gagggtgccc tgctcggccg cccgcatcac cccgcaggtc agcagcaccg
      241 cgttctggta gcggtcctcg ggtgcgctgg cgaagccgcg ggcggccgcg tcgaagcagg
      301 cgtcggcctc accgatccgc ccgagggccg tcagcgccag cccggcgatc acctgcaaca
      361 gcacctgcgc ctgctgcccg gcgacccccg gaccacgcgc cgccagctcc tggatcagcg
      421 gctgcaaggc ggcgagcgcc gcctcgtcgc gcccgcgcag ccacaggttg aagccgagca
      481 cctcgccggt cgcggtctgc ccccaggtgt cgagccagcg ccgctcgtgc gcgtggatct
      541 gccggccgat cgcctcgcac acccgcggct ccccggcgaa ctcgtacacg accatcagcc
      601 gctcgtgcac ccgcgccagc gtcggctggc cgagccgctg cccgagcgcc tcggccgcgc
      661 gcgcgtgggc caggccgcgg gcgcgatgcc cgagcgagac cagcagcgtc gcataattgc
      721 agtacgccat cgccagctcg ggcccctcgc cgagccgctc ggcgacgtag tagtggcgca
      781 gcaccgcctg gaccatcgtt cgccggtcga acgcgaggta ggccatcacg taggcctcgg
      841 tcagatacgc ggcgaccagg gccccgtgcg cgcgcgccgg tccacgcagg gtcccgcgcc
      901 cgaccccggt cgcgccgacg atcaccgcga gcaaccacca ccacgcgctc gccaccgcgt
      961 cgatcggccg gtgtcccggc aagcgctcgc cgagcgcggc aaacgccccg gccatcgtcg
     1021 ccagcgcccg cccgtggtcg cgccgcagct gcgccgcccg ggcccgccgg cgcagcaggg
     1081 ccgagcgccg ccacggctcg tgcgccgtcg ccagcccgcg gtcgatgtgc tgctccgcct
     1141 cgacgagccg acccgtgcgc aggcaggcct ccgcgagcgc gtcgaccagg gccggctcgt
     1201 cggccccggc gaagccctgc gcgcgcgaca ggaaccggtg ggcctcctcg tccgcggcct
     1261 cgctcaacgc gaacagggcc gcggcgagac acgcctcctt caccgccctc gtgtcgcgca
     1321 gcgtgccggc gaggtagtga tgcgcgcgcg cgtagacctc gcgcggccca agcgtcgcgg
     1381 cgaccgcctg gtgcagcgcc tggcgctgtt cgaccgccag cgcggcgagc agcgcctcgc
     1441 gcacgcggtg atgcacgaag ttgtagccgg cccgcccgcg ctcgatcagc ccggccgcgt
     1501 cggcctcgtc gatcgccgcc tcgacctcgg ccgccgcgat cgccccggcg cgggccagca
     1561 gcgccggctc gaagcgatcg ccgcagaccg ccgccaggcg cagcacctca cctgtccgct
     1621 cggacaggtg agcgatccgc ccggagatca gccgcagcgc atcgccgccg agcggcagcg
     1681 cggccagggc ctccgcgtcg gcgacccaca cgtcccagct cgggtgcagc acgccctcgt
     1741 cgagcatcac ccgcaccagc tcgagcaccg ccagcggcat gccgtcgctg cgccgggcga
     1801 ccagctcgac cagctccggg gggatcctcc gcccgccgag caggccggcc atcagctggc
     1861 cgacggccat ggtcggcaag gggccgagcc acaggtacag gctcgccggc aacagctcgg
     1921 cgaagcgaga ccacgccccg cggctgcgcg ccgccggccg cggccccgag cgccggccca
     1981 gcggacccga acccggccag ctgcggccct ccggcacatc gaccggcccc gagcgggccc
     2041 ggtcgcgcgg ccgtggcgcg tccggctcgg cccccagcgc ggccggcatc ggccccgaac
     2101 cgccgcgcgc ggccggcaac ggccccgagc cgccgcgcgc ggccggcagc ggccccgagc
     2161 cgattcgctc gccgccggtc tccccgcgca cgcagcacag cagcagcgct ggcccctcga
     2221 gcgagcgcgc cgcgaagcgc ccgagcagcc gcgcgctgcc ctcgtcgatc cactgtagat
     2281 cgtcgacgat ccacaccagg ccgcccgtgg ccgtcagtcg ggcgagaccg tcgaccaccg
     2341 cgccgtcgac ctgctcgcgc gtcagcttgg ggtccccgcg cgccggcgga ccggcgacca
     2401 gcgtcgccag ggccggcgag agcccgcgca gcagcccggc cagctcggcc agcgccgtgc
     2461 gcaggcgggc cgccagctcg cgcgcgaccg ccgtgtcggc gctggcccgc agcgccaccc
     2521 cgtcgagcgc ctcgcgcacg gccgcgaacg gcaagccgtc gtccgagcag cggctgcgca
     2581 gcacgatcgc cgccgagggt cccgcgacgc ggtcgatgaa ctcggcgacc agccggctct
     2641 tgcccgcccc cgcctcgccc tccatcgcca cgatcaccgg cagagccccg gcccccgtcg
     2701 ccagccgctc ggcggtccgg cgcgtctgct gccacgcctg cgtcagctgt tgcagctccg
     2761 gcccgcgccc gaccagcgcc gtcgccggcc tgtcctccgg gacagcctcg ggcaccagcc
     2821 cgagcaacgg cgccagcgcg gccgccaccg cctcgccggt ctggtagcgg tcgtccgggt
     2881 ccttggccag cagccgcgcg accacctcgg ccagcaccgg cgagcactgc ggacgcacgc
     2941 tcgccagcgc cggcgccggc acgaccgcgt gcaggcgcag cagctcggcc gcgtcggccg
     3001 cgacgaacgg cgggcgcccc gacgccgcgt gaaacagcag agccccgagg gcatacaggt
     3061 cggcgcgccc gtcgacgcca cgaccgacca gccccg
//
LOCUS       DASWNJ010001369         4013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_446055, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001369 DASWNJ010000000
VERSION     DASWNJ010001369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446055"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="VGB85_22805"
     CDS             complement(<1..1193)
                     /locus_tag="VGB85_22805"
                     /inference="COORDINATES: protein motif:HMM:NF018516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HEY0136938.1"
                     /translation="MDSLDSAPVTSPALLPPLLARIDALVAVTGAASSVPSDDRYHDL
                     RQEVDKLSALAGGEVDWRKIAALGDTLTLEVGKDLGVIAYVALARQRMLELDGLLAGL
                     YSLARLLRAPPALLTPAKPRGRASAIEWLLARIHSAVQTQALPPAWAADLEPAIRELR
                     GAAREALGDLAPSFGPLMQAMHGLRERAGPESVAPEASPVAPAAGSPASSVPLAVPAS
                     PGPSAPLAGPVTASAVPSVTLAVPAVEPEPASVPAAPACFAEAAAWLAPLAGASPCGV
                     DPAGSEAFIDVQDQLARLASPSGGAVDWARVEAGSSAVLLGLSKDLRAAIWFTLARAH
                     RGGPTGLAVGLAVVIGLLETFGEALYPRKARSTRDQSEWLIKHAATALAGSQATVEAP
                     LLATLR"
     gene            complement(1213..2646)
                     /gene="tssC"
                     /locus_tag="VGB85_22810"
     CDS             complement(1213..2646)
                     /gene="tssC"
                     /locus_tag="VGB85_22810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HEY0136939.1"
                     /translation="MTPLCDELLRDCPPEGLARARLGVTSVLRTVSADTPASALDDVL
                     LDLLISDCDRRMAAQLDEILHDPQFQALEATWRSLELVVRRVEFDENIRVEILQCRKQ
                     DLLDDFAEAPELPRSGLYQIVYGRAIGTYGAHPYGLLCADFDFGPGAEDVSLLRQCSA
                     IAAMAHAPFIANVDPALFHLPDFTALPRLRDFAAALGGPRLRAWDSLRGTEDARYLGL
                     CLPRVLLREPHDLHTDPRAPLPYRETVQRHRELLWGRPSYVFATLAAGSFARHRWCVH
                     VLGSRAGAVAAQLRWDYPSLAGLWHRVPLECLLSSRVEQALAEGGLIGLVYERSSECV
                     RLVSAASVQRPRQVFTSTEEERAALAGERLGSQLPYIFLVSRLAHYLKCVQRERIGTW
                     SDRSALQRELELWLRQFVSDQDDAQWEVRARRPLRRASVLVEAVEGQTGWYRCRLQLQ
                     PHLTHNSASFTLSLFGKLDRPTGESST"
     gene            complement(2643..>4013)
                     /locus_tag="VGB85_22815"
     CDS             complement(2643..>4013)
                     /locus_tag="VGB85_22815"
                     /inference="COORDINATES: protein motif:HMM:NF045283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion IcmF C-terminal
                     domain-containing protein"
                     /protein_id="HEY0136940.1"
                     /translation="EELRHLYAERYIQAWSDFFERLRLDSPANWTEAVAITTDLTRQD
                     PLKIVFQAIADNTQKLPPIDCSEKPKGLAKWVPMAPPPVNPIARNSTDVAARFARLVA
                     FAFPPAPPPGSQGTAATARLASYQQRLAELRAAAEKAKANPTEANVAGEAATGAQSHL
                     HELVLGASLGNWQESVERLLRPPLDGIALLSVEGVVGKLNREWCDAVVRPIEQTIAGL
                     YPFAPGARIDARLDSIKLLFAPQTGVIAKFRQDNLTGYVEDHGNEIHVRDIGAGAPLH
                     LNRSVVELLDAAHKLGLLLYRDAEIGVDMNVLLSCEQSIQKVVLTIDDVQHEYLCTKN
                     SARQIQWPGKGSGPHQALLDVKGENGRNDRKINVGDFGLIRMLEDGGPRRRAGHNSFA
                     LVYDFKRFNLGNLDMVVDPTPTRGGDVFHGFGVGARFLAPFRAAGFVSPPHSLFAESG
                     YSCGAP"
BASE COUNT          568 a         1306 c         1599 g          540 t
ORIGIN      
        1 cgcagggtgg cgagcagcgg cgcctcgacg gtcgcctggg acccggcgag cgcggtggcg
       61 gcgtgcttga tcagccactc gctctggtcg cgggtcgacc gggccttgcg cggatacagg
      121 gcctcgccga aggtctcgag caggccgatc acgaccgcga gcccgaccgc gaggcccgtc
      181 ggaccgccgc ggtgcgcccg ggccagcgtg aaccagatcg cagcgcgcag gtccttgctc
      241 aggcccagca gcaccgcgct gctgccggcc tcgacgcgcg cccagtcgac cgcgccgccg
      301 ctcgggctgg ccagcctggc gagctggtcc tgaacgtcga tgaacgcctc gctgccggcc
      361 gggtcgacac cgcaggggct cgcgccggcg agcggcgcga gccatgcggc ggcctcggcg
      421 aagcaggccg gcgcggccgg tacactggcg ggctcgggct cgaccgcggg cacagcgaga
      481 gtcaccgacg gcaccgcgct cgccgtgaca ggacccgcga gaggcgctga cggccccggg
      541 ctcgcgggga cagcgagagg caccgacgac gccgggctcc ctgccgcggg cgcaacaggc
      601 gaggcctccg gtgcgacgga ctcgggcccg gctcgctcac gcaggccatg catggcctgc
      661 atgagcgggc cgaagctcgg ggcgaggtcg ccgagcgcct cgcgtgcggc tccgcggagc
      721 tcgcggatcg cgggctcgag gtcggcggcc caggcgggcg gcaacgcctg cgtctgtacg
      781 gcgctgtgga tgcgcgccag cagccactcg atcgcgctgg cgcggccccg cggcttcgcg
      841 ggggtcagca gcgccggtgg ggcgcgcagc agccgggcga ggctgtacag gccggcgagc
      901 aggccgtcga gctcgagcat ccgctggcgg gcgagcgcga cgtaggcgat cacaccgagg
      961 tccttgccga cctcgagcgt cagcgtgtcc ccgagcgcgg cgatctttcg ccagtcgacc
     1021 tcgccgccgg cgagcgcgct cagcttgtcg acctcctggc gcagatcgtg atagcggtcg
     1081 tcggagggga cgctgctcgc ggcgcccgtg acggcgacga gggcgtcgat ccgggcgagc
     1141 agcggcggca agagtgcggg cgacgtgaca ggggccgagt cgaggctgtc catgcgtgaa
     1201 ctgtcttttg gatcaggtgg acgattcgcc cgtcgggcga tcgagcttgc cgaacagcga
     1261 gagcgtgaac gaggcgctgt tgtgggtcag gtgcggctgg agctggaggc ggcagcggta
     1321 ccatccggtc tggccctcga cggcctcgac gagcacggac gcgcgacgca gcgggcgacg
     1381 ggcgcggacc tcccactgcg cgtcgtcctg gtcggagacg aactgccgca gccacagctc
     1441 gagctcgcgc tgcagcgccg accggtcgct ccacgtgccg atccgctcgc gctggacgca
     1501 cttcagatag tgcgcgagcc gggacacgag gaagatgtag gggagctggg acccgaggcg
     1561 ctcgcccgcg agggcggctc gctcctcttc ggtgctggtg aagacctggc gcgggcgctg
     1621 cacggatgcg gcggagacga ggcgaacgca ctcgctgctg cgctcgtaga cgaggccgat
     1681 caggccgccc tccgcgagcg cctgctcgac gcgggacgac agcaggcact cgagcgggac
     1741 gcggtgccac aggccggcca gcgacggata gtcccagcgc agctgggccg cgacggcgcc
     1801 ggcgcgcgat ccgaggacgt gcacgcacca gcgatgccgc gcgaacgacc ccgcggcgag
     1861 ggtggcgaac acataggagg gtcggcccca cagcagctcg cggtggcgct gaacggtctc
     1921 gcggtagggc agcggcgcgc gcgggtcggt gtggaggtcg tgcggctcgc ggagcaggac
     1981 gcgggggaga cacaggccga ggtagcgcgc gtcttcggtg ccgcgcaggg agtcccaggc
     2041 acgcagacgc gggccgccga gcgcggccgc gaagtcacgc agccggggga gcgcggtgaa
     2101 gtcgggcaga tggaacaggg cggggtcgac gttggcgatg aagggcgcgt gggccatcgc
     2161 cgcgatcgcg ctgcactggc gcaggaggct gacatcctcg gcgccggggc cgaagtcaaa
     2221 gtcggcgcac agcagcccgt aggggtgcgc gccgtacgtc ccgatcgcgc ggccatagac
     2281 gatctgatac aggcccgagc gcggcagctc gggggcctcg gcgaagtcgt cgaggaggtc
     2341 ctgcttgcgg cattgcagga tctcgacgcg gatgttctcg tcgaactcga cgcgcctgac
     2401 gacgagctcg aggctgcgcc aggtcgcctc gagcgcctgg aactgcgggt cgtgcaagat
     2461 ctcgtcgagc tgggcggcca tgcggcggtc gcagtcggag atcaggaggt cgagcaggac
     2521 gtcgtcgagc gcgctcgcgg gggtgtccgc ggagaccgtg cgcagcacgg aggtcacgcc
     2581 gaggcgcgcg cgcgcgaggc cctccggcgg gcagtcgcgc agcagctcgt cgcacagcgg
     2641 ggtcatggcg cgccgcagct gtagccggac tcggcgaaca gcgagtgcgg cggggagacg
     2701 aagcccgcgg cgcggaaggg ggccaggaag cgcgcgccga cgccgaagcc gtggaacacg
     2761 tcgccgccgc gggtgggcgt cgggtcgacg accatatcga ggttgccgag gttgaagcgc
     2821 ttgaagtcgt agacgagcgc gaagctgttg tgaccggcgc gccggcgcgg gccgccgtcc
     2881 tccagcatgc ggatcaggcc gaagtcgccg acgttgatct tgcggtcgtt gcgtccgttc
     2941 tcccccttga cgtcgagcag cgcctggtgc gggccggacc cctttccggg ccactggatc
     3001 tggcgggcgc tgttcttggt gcacaggtac tcgtgttgga cgtcgtcgat cgtcagcacg
     3061 accttctgga tcgactgctc gcaggagagc aggacgttca tgtcgacgcc gatctcggcg
     3121 tcgcggtaga gcagcaggcc gagcttgtgc gcggcgtcga gcagctcgac gacgcttcgg
     3181 ttgaggtgca aaggggcgcc ggcgccgatg tcccgcacgt ggatctcgtt gccgtgatcc
     3241 tcgacgtagc cggtcaggtt gtcctggcga aacttggcga tcacgccggt ctggggcgcg
     3301 aacaggagct tgatgctgtc gagccgcgcg tcgatgcggg cgccgggcgc gaaggggtac
     3361 agacccgcga tcgtctgctc gatcgggcgg acgaccgcgt cgcaccactc gcggttgagc
     3421 ttcccgacga caccttcgac ggagagcagg gcgatgccgt cgagcggcgg cctgagcagg
     3481 cgctcgacgc tctcctgcca gttgccgagg ctcgcgccca ggaccagctc gtggaggtga
     3541 ctctgggcgc cggtggccgc ctcaccagcg acgttggcct cggtcgggtt ggccttggcc
     3601 ttttcggcgg cggcgcgcag ctcggcgagg cgctgttgat agctggcgag acgggcggtg
     3661 gccgcggtgc cctggctgcc ggggggcgga gccggcggga acgcgaaggc gacgaggcgg
     3721 gcgaagcggg ccgcgacgtc ggtcgagttg cgggcgatcg ggttgaccgg cggcggggcc
     3781 atcgggaccc atttggcgag ccccttcggc ttctcgctgc aatcgatcgg cgggagcttc
     3841 tgtgtattgt cggcgatcgc ctggaacacg atcttgagcg ggtcttgccg ggtgaggtcg
     3901 gtggtgatgg cgacggcctc ggtccagttg gcggggctgt cgaggcgcag gcgctcgaag
     3961 aagtcgctcc aggcctggat gtagcgctcg gcgtagaggt ggcgcagctc ctc
//
LOCUS       DASWNJ010001370         2202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_307873, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001370 DASWNJ010000000
VERSION     DASWNJ010001370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307873"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1676
                     /locus_tag="VGB85_22820"
     CDS             <1..1676
                     /locus_tag="VGB85_22820"
                     /inference="COORDINATES: protein motif:HMM:NF016488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HEY0136941.1"
                     /translation="ADRVFWSDQRFGQVELRATLRTPYQAYKLAEDVDIEIDKVSSMV
                     SFQVITRDRASCYNALGVLHGWFTPVPGRFRDYIALPRPNRYQALHTAVINRQGVRLE
                     LQIRSAAMDAVAERGIVAEWQRGGVKDGGWQRLTWLQGLMDWQSDVQDPHEFIEAVKA
                     DLFADEVYVFTPEGDIHTFPKGATPIDFAFAIHTDVGVHCSGARVNGHLVPLRYQLRQ
                     GDTIEIITNPAPCVRKEWLKMCHTSRARARIKQLLRQEERLRVRALGRSLVEQELASR
                     GRTLAEFEELGLVARRAAEFDLGKDLRDVPGADGLYEAVGGGQLASLRVADALAPEHE
                     PGADESLIRRMFRRMSGSKIGGKVATGLGSSFGTTIPGFGSNFGNKGGKGPQPEPGEG
                     APGSPMIITRERTEGAGSSAPMIQLAPCCSPVPGDPLIGYFAPGKGIVAHVESCPEAL
                     ERVEGRRVYLAWQAELELESPAIVEVRTANAVGLLAEMSRAFSHHGINIKQANCRTYD
                     NGQRAMNTFHTSVRSLAQLSSLMRTLKSIPGVLGVERVFSRTAAAAGAD"
     gene            1780..1986
                     /locus_tag="VGB85_22825"
     CDS             1780..1986
                     /locus_tag="VGB85_22825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136942.1"
                     /translation="MASFSKVTESKRSRRHRNAGHARKVKLSRKSTPTYQELFAALGE
                     PGQAAPKGASSSKPAAGSPASTKA"
BASE COUNT          354 a          821 c          722 g          305 t
ORIGIN      
        1 aggccgaccg cgtgttctgg tccgaccagc gcttcggcca ggtcgagctg cgcgcgacgc
       61 tgcgcacgcc ctaccaggcc tacaagctcg ccgaggacgt cgacatcgag atcgacaagg
      121 tctcgagcat ggtcagcttc caggtgatca cccgcgaccg cgccagctgc tacaacgcgc
      181 tcggggtcct gcacgggtgg ttcacgccgg tgccgggccg gtttcgcgac tacatcgcgc
      241 tgccgcggcc caaccgctac caggccctac atactgcggt gatcaaccgc cagggcgtgc
      301 gcctcgagct gcagatccgg tcggcggcga tggacgcggt cgccgagcgg ggcatcgtcg
      361 ccgagtggca gcgcggcggc gtcaaggacg gcggctggca gcgcctgacc tggctgcaag
      421 ggttgatgga ctggcagagc gacgtccagg acccgcacga gttcatcgag gccgtgaagg
      481 ccgacctgtt cgccgatgaa gtctacgtgt tcaccccgga gggcgacatc cacaccttcc
      541 ccaagggcgc cacgccgatc gacttcgcgt tcgctatcca caccgatgtc ggcgtgcact
      601 gctcgggcgc gcgggtcaac ggccacctcg tgccgctgcg ctatcaactg cgccagggcg
      661 acacgatcga gatcatcacc aacccggcgc cgtgcgtgcg caaggagtgg ctcaagatgt
      721 gccacacctc gcgggcccgc gcgcggatca aacaattgct gcgccaggag gagcgcctgc
      781 gcgtgcgagc gctcggccgc agcctcgtcg agcaggagct cgccagccgc gggcgcacgc
      841 tcgccgagtt cgaggagctc ggcctcgtcg cccgccgcgc cgccgagttc gacctcggca
      901 aggacctgcg cgacgtcccc ggcgccgacg ggctgtacga ggccgtcggt ggcggtcagc
      961 tcgcgtcgct gcgcgtcgcc gacgcgctcg ccccggagca cgagcccggc gccgacgaga
     1021 gcctgatccg ccgcatgttc cggcgcatgt cgggcagcaa gatcggcggc aaggtggcga
     1081 ccggcctcgg cagcagcttc ggcaccacga tccccggctt cgggagcaac ttcggcaaca
     1141 agggcggcaa gggcccgcag cccgagccgg gggagggcgc cccgggatcg ccgatgatca
     1201 tcacccgcga gcgcaccgag ggcgcgggat ccagcgcgcc gatgatccag ctcgcgccgt
     1261 gctgctcgcc ggtcccgggc gacccgctga tcggctactt cgcgccgggc aaggggatcg
     1321 tcgcccacgt cgagagctgc ccggaggcgc tcgagcgcgt cgagggccgc cgcgtgtacc
     1381 tcgcctggca ggccgagctc gagctcgaga gcccggcgat cgtcgaggtc cgcaccgcca
     1441 acgcggtcgg cctgctcgcc gagatgagcc gcgccttctc ccaccacggc atcaacatca
     1501 agcaggccaa ctgccgcacc tacgacaacg gccagcgggc gatgaacacc ttccacacct
     1561 cggtgcgctc cctcgcccag ctcagctccc tcatgcgcac cctcaagagc atccccgggg
     1621 tgctcggcgt cgagcgggtg ttctcacgca ccgcggccgc cgcgggcgcg gactgacgta
     1681 tttttctccc tcgatccctt cccaaatgcc cgcaaagggc gttgacaagc acgcgacgcc
     1741 cggttatgcg tgcaccccct ccgcacagga ctcaccgcga tggcttcatt ctccaaagtt
     1801 accgagagca agcgctcccg ccgccacagg aacgcgggcc acgcccgcaa ggtcaagctc
     1861 agccgcaaga gcacgcccac ctaccaggag ctgttcgcgg cgctcggtga gcccggccag
     1921 gccgctccca agggcgccag cagcagcaag cccgccgccg ggtccccggc gtcgaccaag
     1981 gcctgagcct gtccccgacc tgcgcgcgcg caggtccttt gtccaccttc agatcaccag
     2041 gcgagggacg tgcaccaacg ggcatgtccc ttgttcgtgt tcggtgcata cgcagctgga
     2101 tcctccccgc acggaccgca gcccccgtga acgcggcatc tttggccgtc cgggcgatcc
     2161 atgcgaaggt tgcgcgcgcg cttgatcaac catgcaccat tg
//
LOCUS       DASWNJ010001371         3161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_517391, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001371 DASWNJ010000000
VERSION     DASWNJ010001371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517391"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VGB85_22830"
     CDS             complement(<1..582)
                     /locus_tag="VGB85_22830"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0136943.1"
                     /translation="MRPNLPGATFSGDDLGATQAPPGDTLVPARAGTPDAPNPGETLH
                     AFPVPLAPTGASHPDADLNLSTERTTLRVGTSATISGLDKTAVGSSGSRPSLEETLPR
                     TGDPRRIHRFAVLRQLGAGGMGVVYAGYDEELERRVAIKVVREDMVASQGRSRMLREA
                     QAMAKVSHPNVVQVYEVGEFAGQVFVAMEFVKGI"
     gene            complement(696..2036)
                     /locus_tag="VGB85_22835"
     CDS             complement(696..2036)
                     /locus_tag="VGB85_22835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0136944.1"
                     /translation="MNDNTKMSSKQIVEDCKQYTMWSWSKGSAVNPLAIERAEGVYLY
                     TTDGKRYIDFNSQLMSVNIGHSHPRVLDAMKRAIDSQLLFVAPGLATAPRARLGKRLA
                     RLVPGDINTFFFTNGGAEANENAVKAARLFTGRHKILSRYRSYHGATHAGMMMTGDPR
                     RLPNEPGAPGFIRVMDPVPYDYSFGDTDEQRTENHLRYLEETITYEGPGTIAAMIIET
                     VIGTNGILPPPRGWLKGLRALLDKYGILLICDEVMCGFGRTGKMFAFEHGDIVPDILT
                     MAKGLTSSYLPLGAMGVNDKIAKHFQDNVFFGGLTYNAHALCLATAEAVLDVMEDENL
                     VDNAARMQEVMLAEMAALAHKHPSVRNFRAIGLFGLIELQKDRQGTRMAPYGGSHPAI
                     DKLMGFFKQEGLYTISHWWNIMCNPPLSITPEQLREGFEIIDRGLEITDAAVEG"
     gene            complement(2048..>3161)
                     /locus_tag="VGB85_22840"
     CDS             complement(2048..>3161)
                     /locus_tag="VGB85_22840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0136945.1"
                     /translation="GLSLENMAQGEQLDVSRGINCSTTHEPLGVVAGVVPFNFPVMVP
                     LWMLPQALAAGNTFVLKPSEVVPYGAMKLASLLQEAGLPDGVFNTVNGGQPTVEGLVD
                     HLDVKAMAFVGSTKVARLLYARGAALGKRMLCLGGAKNHLLVVPDADPELTASTVVAS
                     AYGCAGQRCMAASLMIAVGDIDPIIESIASITRKIRLGEDMGPVIHAAAALRIHRYID
                     QAEQMGAKVLVDGRGAKGPDGGHWVGPTILDHVRPDMPAGCEEIFGPVLSIVRARTVD
                     EAIAIENASSYGNAASIFTSSGAVARHCIERFEAGMCGINVGVPVPREPFGFGGWNDS
                     LFGQGDLTGYDGFRFWTRARKLTSRWSLSKDATWMS"
BASE COUNT          462 a         1006 c         1166 g          527 t
ORIGIN      
        1 gatgcccttg acgaactcca tcgccacgaa cacctggccg gcgaactcgc cgacctcgta
       61 gacctggacg acgttggggt gcgagacctt ggccatcgcc tgggcctcgc gcagcatgcg
      121 agagcggccc tgactggcga ccatgtcctc gcgcacgacc ttgatcgcga cgcggcgctc
      181 gagctcctcg tcgtagccgg catacacgac gcccatgccg ccggcgccga gctggcgcag
      241 caccgcgaag cggtggatcc ttcgcgggtc gccggtgcgc ggcagcgtct cctcgagcga
      301 gggccggctg ccggagctgc cgaccgcggt cttgtcgagg cccgagatgg tcgccgacgt
      361 gcccacgcgc agcgtggtgc gctcggtgct caggttgagg tcggcgtccg ggtgcgaggc
      421 gccggtcggc gccagcggga ccgggaaggc gtgcagggtc tcgccggggt tcggcgcgtc
      481 gggggtacct gcgcgagcgg gcacgagggt gtccccggga ggggcctgcg tggcccccag
      541 gtcgtcgcct gaaaaggtcg cacctggcag gttcgggcgc atggggaagg gtcgcaagcg
      601 ggggcgtgcg gcgcaagttc cgtctgtcgc ctgaacgccg cggagattac agcgggcacg
      661 tcgccgcggc ggggcgcggt agtgcccgtg ctcgtttagc cctcgacggc ggcgtcggtg
      721 atctcgaggc cgcggtcgat aatctcaaag ccctcgcgca gctgctcggg ggtgatcgac
      781 aggggagggt tacacatgat gttccaccag tgggagatcg tgtagaggcc ctcctgcttg
      841 aagaagccca tcagcttgtc gatcgccggg tgcgagccgc cgtacggggc catgcgcgtg
      901 ccctggcggt ccttttgcag ctcgatgagg ccaaagaggc cgatcgcgcg gaagttgcgg
      961 acgctggggt gcttgtgcgc gagcgcggcc atctcggcga gcatgacctc ttgcatgcgc
     1021 gcggcgttgt cgacgaggtt ctcgtcctcc atgacgtcga gcacggcctc ggcggtggcg
     1081 aggcacagcg cgtgggcgtt gtaggtcagg ccgccgaaga agacgttgtc ctggaagtgc
     1141 ttggcgatct tgtcgttgac gcccatcgcg ccgagcggca agtacgagct ggtcaggccc
     1201 ttcgccatcg tcaagatgtc ggggacgatg tcgccgtgct cgaaggcgaa catcttgccg
     1261 gtgcgcccga agccgcacat gacctcgtcg cagatcagca ggatgccgta cttgtcgagc
     1321 agcgcgcgca ggcccttgag ccagcccctc ggcggcggga ggatcccgtt ggtgccgatc
     1381 acggtctcga tgatcatcgc cgcgatcgtg cccgggccct cgtaggtgat cgtctcctcg
     1441 aggtaacgca ggtggttctc cgtgcgctgc tcgtcggtgt cgccgaacga gtagtcgtag
     1501 gggaccgggt ccatcacgcg gataaagccg ggcgcgccgg gctcgttggg caggcggcgc
     1561 gggtcgccgg tcatcatcat gccggcgtgg gtcgccccgt ggtagctgcg gtagcggctg
     1621 aggatcttgt ggcggcccgt gaacaggcgc gccgccttga ccgcgttctc gttggcctcg
     1681 gcgccgccgt tggtgaagaa gaaggtgttg atgtcaccgg gcacgagccg cgcgagccgc
     1741 ttgccgaggc gcgcccgcgg ggcggtggcg aggccggggg cgacgaacag cagctggctg
     1801 tcgatcgccc gcttcatcgc gtcgagcacg cgcgggtgcg agtggccgat gttgacgctc
     1861 atcagctggc tgttgaagtc gatgtaacgc ttgccgtcgg tcgtatagag gtacacgccc
     1921 tcggcgcgct cgatggccag cgggttgacc gcgctgccct tcgaccacga ccacatcgtg
     1981 tactgcttgc aatcttcgac gatctgcttg ctgctcatct tggtgttgtc gttcatgacg
     2041 gtctccttca cgacatccag gtcgcgtcct tgctcaggga ccagcgagag gtcagcttgc
     2101 gcgcccgggt ccagaagcga aagccgtcgt agccggtcag gtcgccctgg ccgaacaggc
     2161 tgtcgttcca gccgccgaag ccgaagggct cgcgcgggac gggcacgccg acgttgatgc
     2221 cgcacatgcc ggcctcgaag cgctcgatgc agtgacgagc caccgcgccg gagctggtga
     2281 agatcgaggc ggcgttgccg tagctgctgg cgttctcgat cgcgatcgcc tcgtcgacgg
     2341 tgcgcgcgcg gacgatcgag agcaccggcc cgaagatctc ctcgcagcca gcgggcatgt
     2401 cggggcggac gtgatcgagg atcgtcgggc cgacccagtg gccgccgtcg gggcccttgg
     2461 cgccgcgtcc gtcgacgagc accttggcgc ccatctgctc ggcctggtcg atgtaacgat
     2521 ggatgcgcag ggcggcggcg gcgtggatca ccgggcccat gtcctcgccg agacggatct
     2581 tgcgcgtgat gctcgcgatc gactcgatga tcggatcaat atcaccgacg gcgatcatca
     2641 gcgaggcggc catgcagcgc tggccggcgc agccgtacgc ggaggcgacg acggtgctcg
     2701 cggtcagctc cgggtcggcg tcaggcacga cgagcaggtg gttcttggcg ccgccgaggc
     2761 acagcatgcg cttgccgagc gcggcgccgc gggcgtacag caggcgcgcg accttggtcg
     2821 agccgacgaa ggccatcgcc ttgacatcca agtggtcgac gagcccctcc accgtgggct
     2881 gcccgccgtt gacggtgttg aacacgccgt cgggcaggcc cgcctcctga aggagactcg
     2941 cgagcttcat cgcgccataa ggcacgacct cggagggctt gagcacgaag gtgttcccgg
     3001 cggcgagcgc ctgcgggagc atccacagcg ggaccatgac ggggaagttg aagggcacga
     3061 cgccggcgac cacgccgagc ggctcgtggg tggtgctgca attgatgccg cggctgacgt
     3121 cgagctgttc gccctgggcc atgttctcga ggctgaggcc g
//
LOCUS       DASWNJ010001372         2718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_376122, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001372 DASWNJ010000000
VERSION     DASWNJ010001372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376122"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1285)
                     /locus_tag="VGB85_22845"
     CDS             complement(83..1285)
                     /locus_tag="VGB85_22845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY0136946.1"
                     /translation="MSETRTMGSASEPLPAAVDVAIVGAGIMGLALAYHLARRARGLK
                     IAVIERSYLVSGASGRNGGGLRMQWGDAGNVRLMMESIDECKGLAQDLGINLWFRQGG
                     YLILARNEAGEQRLIRNVELHAEVGAPTRLLSAAEAQAIVPQLDVSEVRLATFNPEDG
                     VVFPWPFVWGYASQAVKHGVALRTHTAVTRVEPTGDRYTLQLSTGERLTADRVVNATG
                     AWSHGLNHGLGIALPNRPHRHEILSSEPLKPFLDPLVVDLGSGLYFSQSTRGELVTGI
                     TLPDAADGSDTALTLHSSLRFLGHISRTLTRLMPVVAGMKVLRQWVGPYDVSPDGDAI
                     VGPSPGHPKVIQVCGFTGHGFMMAPAVGRLLAHWLADGTTHPMLTRWDPSRFASGRPL
                     RREDMIIG"
     gene            complement(1282..1569)
                     /locus_tag="VGB85_22850"
     CDS             complement(1282..1569)
                     /locus_tag="VGB85_22850"
                     /inference="COORDINATES: protein motif:HMM:NF037640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY0136947.1"
                     /translation="MSDRSFVCRCEDVTLAELDHTIARGLATVEEIKRYTGLGTGPCQ
                     GKECVPLLCRILVDRGLLTPATAQPFTARPPVEPVTFATLAASQEEEETGP"
     gene            complement(1569..>2718)
                     /locus_tag="VGB85_22855"
     CDS             complement(1569..>2718)
                     /locus_tag="VGB85_22855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY0136948.1"
                     /translation="DCGTCWVRVDGQPNVRACLTPVRDGMQVASQNSYGDLDPTALID
                     AVFPGGIDHHHLVVYPRVANTVMQGVARNLTGFGSLPDHAPVTDAWREDHAPEVLIVG
                     AGRSGRAAAATLIAAGLDVLVLDRRDATALTAWDPAPVPASVRAGVGVFAAYPHEGLW
                     AAAAASGEDGAAPTLHIIRPRHVILALGSHLPTLPVPGNDVPGVLSARGLVHLLARTG
                     RRPHGPVVVIGAGATAELYAQQLGATLVAPAAVKKIVGRRRVEAVETFNGQITGRITA
                     RLVALAPAPAPASELAAQAGAALRWNGDGFAPVCDADGRVQLRAEDHPLAGPASFDVG
                     TILDGPPAPPTPAWTLWACGSLRATGDDGAAVARALLRHMQPTQAGGQ"
BASE COUNT          351 a          913 c         1103 g          351 t
ORIGIN      
        1 cgtccgggtc gcgggggcgc ggggatcacc gccgtcaccg ccggtgccgt cgtcgccccc
       61 aggggcacga gggtcgcgga gctcagccga tgatcatgtc ctcgcgccgc agcgggcggc
      121 cgctggcgaa gcgcgaggga tcccagcgcg tgagcatcgg gtgcgtggtg ccgtcggcga
      181 gccagtgcgc gagcaggcgg ccgaccgcgg gcgccatcat gaagccgtgg ccggtgaagc
      241 cgcacacctg gatgaccttc gggtggccgg ggctcgggcc gacgatcgcg tcgccgtcgg
      301 gcgagacgtc gtaggggccg acccactggc gcagcacctt catgccggcg accaccggca
      361 tcagccgcgt cagcgtgcgg ctgatgtggc cgaggaagcg caggctcgag tgcagggtca
      421 gggcggtgtc gctgccgtcg gcggcgtcgg gcaaggtgat gccggtgacg agctcgccgc
      481 gggtgctctg cgagaagtac aggccgctgc cgaggtcgac gacgagcgga tcgaggaacg
      541 gcttgagcgg ctcggacgag aggatctcgt gccggtgcgg ccggttgggc agggcaatgc
      601 cgagaccgtg gttgaggccg tggctccacg cgccagtcgc gttgacgacg cggtcggcgg
      661 tcagccgctc gccggtcgag agctgcaggg tgtagcgatc gccggtcggc tccacgcgcg
      721 tgacggcggt gtgggtgcgc agcgcgaccc cgtgcttgac cgcctgcgac gcgtagcccc
      781 acacgaaggg ccacgggaac acgacgccgt cctcggggtt gaaggtggcg aggcggacct
      841 cggagacgtc gagctgcggc acgatcgcct gggcctcggc ggcgctgagc aggcgggtcg
      901 gggcgccgac ctcggcgtgc agctcgacgt tgcggatcag cctctgttca ccagcctcgt
      961 tgcgcgcgag gatcaggtag ccgccctgac ggaaccagag gttgatgccg aggtcctgcg
     1021 cgaggccctt gcactcgtcg atgctctcca tcatcaggcg cacgttgccg gcgtcgcccc
     1081 attgcatgcg caggccgccg ccgttgcggc ccgaggcgcc gctgacgagg tagctgcgct
     1141 cgatgaccgc gatcttgagg ccgcgcgcgc ggcgggccag gtgatacgcg agcgcgaggc
     1201 ccatgatgcc ggcgccgacg atcgcgacgt cgacggcggc cggcagcggc tcgctcgcgc
     1261 tgcccatcgt gcgggtctcg ctcacgggcc ggtctcctct tcttcctggc tggccgcgag
     1321 ggtcgcgaag gtgacgggct cgaccggcgg gcgcgcggtg aacggctggg cggtcgccgg
     1381 cgtcagcagg ccgcggtcga cgaggatccg gcacagcagc ggcacgcact ccttgccctg
     1441 acaggggccg gtgccgaggc cggtgtagcg cttgatctcc tcgacggtgg cgaggccgcg
     1501 ggcgatcgtg tggtcgagct cggcgagggt cacgtcctcg cagcggcaca cgaagctacg
     1561 atccgacatc actgccctcc ggcctgagtt ggttgcatgt gccgcagcag cgcgcgcgcg
     1621 acggcggccc cgtcgtcgcc ggtggcgcgc agcgagccgc aggcccacag ggtccacgcg
     1681 ggggtcggcg gggcgggcgg gccgtcgagg atcgtgccga cgtcgaagct ggccgggccg
     1741 gcgagcgggt ggtcctcggc gcgcagctgg acgcggccgt cggcgtcgca caccggggcg
     1801 aagccgtcgc cgttccaccg cagggcggcg ccggcctgcg cggcgagctc ggaggcgggc
     1861 gcgggtgccg gggcgagcgc gaccaggcgc gcggtgatcc gtccggtaat ctgtccgtta
     1921 aaggtctcga cggcctcgac ccggcgccgg ccgacgatct tcttcacggc cgcgggggcg
     1981 accagcgtcg cgccgagctg ctgcgcgtac agctcggcgg tcgcgccggc gccgatcacg
     2041 acgacggggc cgtgcgggcg ccgacctgtc cttgcgagca ggtgcacgag gccacgcgcc
     2101 gagagcacgc cgggcacgtc gttgccgggg acggggaggg tcgggaggtg gctgccgagc
     2161 gcgaggatca cgtggcgcgg gcggatgatg tgcagggtcg gggcggcccc gtcctcgccg
     2221 ctcgcggcgg cggcggccca caggccctcg tgggggtagg ccgcgaacac gccgacgccg
     2281 gcgcgcacgc tcgcgggcac cggcgcggga tcccaggccg tcagcgcggt ggcgtcgcgg
     2341 cgatcgagca cgagcacgtc gaggccggcg gcgatcaagg tcgccgccgc ggcgcgaccc
     2401 gagcggccgg cgccgacgat caggacctcg ggggcatgat cctcgcgcca ggcgtcggtg
     2461 acgggcgcgt ggtcgggcaa gctgccgaag ccggtcaggt tgcgcgcgac gccctgcatc
     2521 accgtgttgg cgacccgggg atagacgacc aggtgatggt gatcgatgcc accggggaac
     2581 acggcgtcga tcaacgcggt cgggtcgagg tcgccgtagc tgttctgcga ggcgacctgc
     2641 atgccgtcac gcacgggcgt caggcaggcg cgcacgttgg gctggccgtc gacgcggacc
     2701 cagcaggtgc cgcagtcg
//
LOCUS       DASWNJ010001373         1701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_589867, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001373 DASWNJ010000000
VERSION     DASWNJ010001373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589867"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..1596
                     /locus_tag="VGB85_22860"
     CDS             244..1596
                     /locus_tag="VGB85_22860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136949.1"
                     /translation="MLFRTHTLMAATLAAALASACTNGGDDTTSAGTGSTGSTSTGTS
                     AGPATDPTTQTDTSGDPPTGSTDPTASVTDGTTDPTTTTTGPDPTQGTTTEPGTDATG
                     DPSGDPGGDPTTDTAGDDSTLCASQAEGRYFPDDAWFYQNVTKAPVTADSSAITQWMV
                     QNMGPGGWGTGSMRIDFSIVAIDAPAGTMKRDFVLDENFYYEPDCDTAPVPLPPGGYV
                     EDYPQEPPDPNDQWAGYDCSGFGDGADCHMIVVSRSEQRLYEIYHATLDDTDTLYAGC
                     EAVWSTGAAPGPDGRGQQCTSADAAGFPIAPLLFSAEEVKAGVIDHAIRFILPNSMIR
                     AKKYVAPATHGTNTTGPATSIPYGGHMRLRADYPLENLKPAAQVVARAMQTYGMYMAD
                     GGNIALTAQADVVGCATWAEVDLGPQDLNMLKATDFEVIDHGPTMDVTYECGRTPVTE
                     "
BASE COUNT          302 a          594 c          589 g          216 t
ORIGIN      
        1 gaggcctccg tcggacatcc ggctactcga cagtgcgcgc gcccccagcc ggacccgcgt
       61 cggcccgtgc gacgtcgccc gcgcaccggg ccccagcgtc cgcgcaaaca cggcccccgc
      121 acacgacagt acccaggccc gcacacattc gcgcggaaca aaacagaagt gggtccacgc
      181 gaccccgcgc ccgcccgcga gcggctcgct atacgcgaag cccgccgatg gggatgatgg
      241 cggatgctct ttcgcaccca tacattgatg gccgcgacgc tggcagcggc gctcgcgtcc
      301 gcatgcacga acggcggcga cgacacgacc tccgccggca ccggcagcac tggaagcacc
      361 agcactggca ccagcgccgg ccccgccacg gatccgacca cgcagaccga cacgagtggt
      421 gatccaccga ccggatcgac ggacccgacc gcgagcgtga cggacgggac gacagatccg
      481 acgaccacga cgaccggccc ggatccgacc cagggtacga ccaccgagcc gggcacggat
      541 gcgaccggcg acccgagcgg tgacccgggc ggagatccga cgacggacac cgcgggcgac
      601 gacagcaccc tgtgtgcgag ccaggcggag gggcgttact ttccggacga tgcgtggttc
      661 tatcagaatg tcaccaaggc gccggtgacc gcggattcgt cggcgattac gcagtggatg
      721 gtgcagaaca tggggccggg cggctggggg acgggatcga tgcggatcga cttcagcatc
      781 gtggcgatcg acgcgcccgc ggggacgatg aagcgggact ttgtgctcga cgagaatttt
      841 tattacgagc cggattgcga caccgcgccg gtgccgctgc cgccgggtgg gtatgtcgag
      901 gattacccgc aggagccgcc ggacccgaat gatcaatggg cgggctacga ttgcagcggg
      961 ttcggcgatg gggcggattg ccacatgatc gtcgtgtcgc gctccgagca gcgtttatat
     1021 gagatctacc acgctacgct cgacgacacg gacaccctgt atgccgggtg cgaggcagtg
     1081 tggtcgacgg gcgcggcgcc ggggccggac gggcgcgggc agcagtgcac cagcgcggac
     1141 gcggcgggct tcccgatcgc gccgctgctg tttagcgccg aggaggtcaa ggccggggtc
     1201 atcgaccacg cgatccgctt cattttgccg aactcgatga tccgggccaa gaagtacgtc
     1261 gcgccggcga cccacgggac caacacgacc gggccggcga cctcgatccc gtacggcggc
     1321 cacatgcggt tgcgggccga ctacccgctc gagaacctca agccggcggc gcaggtggtc
     1381 gcccgggcca tgcagaccta cgggatgtac atggcggacg gcggcaacat cgccctgacg
     1441 gcccaggccg acgtcgtcgg ctgcgccacc tgggccgagg tcgatctcgg gccgcaggac
     1501 ctcaatatgc tcaaggcgac cgatttcgag gtcatcgacc atggcccgac catggacgtg
     1561 acctacgagt gcggccggac cccggtcacg gagtaggccc cggggctgcg gcgtgctaag
     1621 acgcgccgca gcgatgaccc gcgcccctgc cactcccccc ggctcgccga gccgcgcgcc
     1681 gatcctcgag ctctcggccg a
//
LOCUS       DASWNJ010001374         2550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_376176, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001374 DASWNJ010000000
VERSION     DASWNJ010001374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376176"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VGB85_22865"
     CDS             <1..756
                     /locus_tag="VGB85_22865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136950.1"
                     /translation="LVEAPDLAETHLARAMLGAQLGEWRETVKALVRALEIAPTYAHA
                     HQYLAQLQCEAGNIKEGLERAKLAHDLEPSLVMSLFDIARLHALHGRREECERVLAMV
                     DRNSRMHSPMIQFRLRVAGWFGDLDAVRDIADDLRNDHTDGFGRYVINYARCLAGELS
                     KQEMDAVTSELVKHSPSPRFYTLMCQSAVEIYAARGYPEDALRYFQSAADRVLIDIEW
                     TDRCPLLASIRGLPGYTEARRKIRQRVQAMWSL"
     gene            753..1793
                     /locus_tag="VGB85_22870"
     CDS             753..1793
                     /locus_tag="VGB85_22870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0136951.1"
                     /translation="MTALELRDIHKAFAHGGALALKGVSLSLPLAAYTCVMGASGSGK
                     TTLLRVVAGLERPDRGEVVLAGKVVTQVPAERRPVHTLFQGYALFPHLSAWENVAFAP
                     RLAGVGGAVLRERVLALLADVGLAPRLHARRPAQLSGGERQRVALARALAGAPTMVLL
                     DEPLAALDRPLRGGLRRLLKETQQRRKIGFLHVTHEPEEALALADHLVLMDQGLVLAQ
                     GGPAALYSRPPDMASARLLGDLTAVPGEPGRWLRPERLAIGDVSGDMSDATGAWRRAL
                     VTLDGVACLGDRWELQLRTEGQRLLARSMNPPASSPGAVVAARWRAEDELALGEAGGR
                     STAGWSSTALRL"
     gene            complement(1844..>2550)
                     /locus_tag="VGB85_22875"
     CDS             complement(1844..>2550)
                     /locus_tag="VGB85_22875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136952.1"
                     /translation="FVAYYTLQFAYAWMVGAFTTLIAVLYSLLGHFSPDLLYLRVLET
                     IVGAGVGAVVAALLYPVHTRPAIRHGMVEALRMLAEHIELVMVHPPAQREALRASARA
                     LDRKLRDLRTEVQRAAGVPLLRGGRERARVFLAFATIYFHARPLVATDWQAADREPLR
                     AVGVGLAANARALADLLAGAGGPDMLSVMPALEAARARLTTPATLRWLAALDDALLTL
                     RREILELDWAGLASKQ"
BASE COUNT          348 a          823 c         1056 g          323 t
ORIGIN      
        1 ctcgtcgagg cgccggacct ggccgagacg cacctggcgc gggcgatgct cggggcccag
       61 ctcggcgagt ggcgcgagac ggtcaaggcg ctggtgcggg ccctcgagat cgcgccgacc
      121 tacgcgcacg cgcatcagta cctcgcgcag ctgcagtgcg aggccggcaa catcaaggag
      181 gggctcgagc gggccaagct ggcgcacgac ctcgagccga gtctggtgat gagcctcttc
      241 gacatcgccc gcctgcacgc gctgcacggc cggcgcgaag agtgcgagcg ggtgctggcg
      301 atggtcgacc gcaactcgcg gatgcacagc ccgatgatcc agtttcgcct gcgcgtcgcc
      361 gggtggttcg gcgacctcga cgcggtgcgc gacatcgccg acgacctcag gaacgaccac
      421 acggacggct tcgggcgcta cgtgatcaac tacgcgcgct gcctcgcggg ggagctcagc
      481 aagcaggaga tggacgcggt gacgtccgag ctggtcaagc actcgccgag cccgcgcttc
      541 tacacgctga tgtgccagtc ggcggtcgag atctacgcgg cgcgcgggta cccggaggac
      601 gcgctgcggt acttccagtc cgcggcggat cgcgtgctga tcgacatcga gtggaccgac
      661 cgctgcccgc tgctcgcgtc gatccgcggg ttgcccggct acaccgaggc gcgtcgcaag
      721 atccggcagc gggtccaggc gatgtggagc ctgtgacggc gctcgagctg cgagacatcc
      781 acaaggcgtt cgcgcacggc ggggcgctcg cgctcaaggg agtgtcgctg tcgctgccgc
      841 tcgcggcata cacgtgcgtg atgggggcct cgggctcggg caagacgacg ctgctgcgcg
      901 tggtcgccgg gctcgagcgg cccgaccgcg gtgaggtggt gctcgcgggc aaggtggtca
      961 cgcaggtgcc ggcggagcgc cggcccgtgc acacgctgtt ccagggctac gccctgttcc
     1021 cgcacctgag cgcgtgggaa aacgtcgcgt tcgcgccgcg gctggcgggg gtcggcgggg
     1081 cggtgctgcg cgagcgggtg ctggcgctgc tcgccgatgt cgggctggcg ccgcggctgc
     1141 acgcgcgccg gccggcgcaa ttgtcagggg gcgagcggca gcgggtcgcg ctggcgcggg
     1201 cgctcgcggg ggcgccgacg atggtgctgc tggacgagcc gctggcggcc ctagatcggc
     1261 cgctgcgcgg cgggctgcgg cggctgctca aggagacgca gcagcggcga aagatcgggt
     1321 tcttgcacgt gacgcacgag ccggaggagg cgctggcgct cgccgatcac ctggtgctga
     1381 tggatcaggg gctggtattg gcgcagggcg ggccggcggc gctgtactcc cggccgcccg
     1441 acatggcgag cgcgcggctg ctcggggacc tgacggcggt cccgggcgag ccggggcggt
     1501 ggctgcggcc cgagcggctg gcgatcgggg atgtctccgg ggacatgtcc gatgcgacag
     1561 gcgcgtggcg gcgcgcgctg gtgaccctcg acggggtcgc gtgcctcggc gaccgctggg
     1621 agctgcagct gcggaccgag ggccagcgcc tgctcgcgcg cagcatgaac ccgcccgcga
     1681 gctcgccggg ggcggtcgtc gcggcgcgct ggcgggccga ggacgagctg gcgctcggcg
     1741 aggccggcgg gcgctcgacg gcggggtggt cgtcgacggc gctgcggctc tgactggagg
     1801 gacaggttcg cgagccgggg cgcgggctga ccgaagggac aggtcattgt ttgctggcga
     1861 ggccggccca gtcgagctcg aggatctccc ggcgcagggt cagcagggcg tcgtcgaggg
     1921 cggcgagcca gcgcagggtg gcgggcgtgg tcaggcgagc gcgggcggcc tccagcgcgg
     1981 gcatgacgga gagcatgtcg gggccgccgg cgccggcgag caggtcggcg agggcgcggg
     2041 cgttggccgc gagaccgacg ccgacggccc gcagtggctc gcggtcggcg gcctgccagt
     2101 cggtggcgac gagcgggcgg gcatgaaagt agatcgtggc gaaggcgagg aacacgcggg
     2161 cgcgctcgcg gccgccgcgg agcagaggca cgccggcggc ccgctggacc tcggtccgca
     2221 ggtctcgcag cttgcggtcg agcgcgcggg cggaggcccg aagcgcctcg cgctgcgcgg
     2281 gtggatgcac catcacgagc tcgatgtgct cggcgagcat gcgcagcgcc tcgaccatgc
     2341 cgtgacggat cgcagggcgc gtgtgcaccg ggtagagcag cgcggcgacg acggccccga
     2401 cgcccgcgcc gacgatcgtc tcgagcacgc gcaggtacaa caggtcgggc gagaagtgac
     2461 ccaggaggct gtagagcacg gcgatcaggg tcgtgaaggc gccgaccatc caggcgtagg
     2521 cgaattgcag ggtgtagtag gcgacgaaca
//
LOCUS       DASWNJ010001375         1635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_376188, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001375 DASWNJ010000000
VERSION     DASWNJ010001375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376188"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1635
                     /locus_tag="VGB85_22880"
     CDS             <1..>1635
                     /locus_tag="VGB85_22880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136953.1"
                     /translation="LVNALRSSKLVRLTAGSEQLELYHDRIRETLAAQIGPDRRPAIH
                     RALADELVSAGADDPEVLFVHYHASGQIEAARGAAVRSAAKATAALAFDQAAQFYQRA
                     LDLGHELGIDHDTASVTSRARLEEGLADALANAGACRPAAAAYQRAASFWQGSKALDL
                     RRLAAEQLLVSGHHAEGRVVLQDVLAEVGLQLPLTPRRALLSLVTRRLQLKLHGMRFA
                     LRREAECDPDQLMLIDICTVVSMGLSMNDNIRAAGFQSYGTLLALRAGEPVRLSRALA
                     MESSLVASSGGPARTHALRLSQAALDLAHQVRSPASIATALLHAGACAYLAGGEWRRA
                     AKLCCESETLFREKVPGSTWAKTTVRRFWLGSLMYLGELAELRRRLAEFLGDARDRGN
                     VYALTDMRARLNLAWLIADEPAEARRQLDDAMRSWPSDGYHVQHFNALLSQVQADLYE
                     GNPAAAVPKIEAVWPALTGSMLMRIQLVRTEAHQLRARAGLATLAASGRPTRDAIKVI
                     EREVDALGKEKMPWIDPLAELLRAGLQRVQGRSDAAV"
BASE COUNT          219 a          684 c          521 g          211 t
ORIGIN      
        1 ctcgtcaacg cgctgcgatc gagcaagctc gtccgcctca ccgcgggcag cgagcagctc
       61 gagctctacc acgaccggat ccgcgagacc ctcgcggccc agatcggccc cgaccgccgc
      121 cccgcgatcc accgcgccct cgccgacgag ctcgtctccg ccggcgccga cgaccccgag
      181 gtcctgttcg tgcactacca cgcgtcgggt cagatcgagg ccgcgcgggg cgctgcggtc
      241 cgatccgccg ccaaggcgac cgccgccctc gcgttcgatc aggccgcgca attctatcaa
      301 cgcgccctcg acctcggcca cgagctcggc atcgaccacg acaccgccag cgtcacctcg
      361 cgcgcccgcc tcgaggaggg cctcgccgac gcgctcgcca acgccggcgc ctgccgcccc
      421 gccgcggcag cctaccagcg cgccgcgagc ttctggcagg gctccaaggc gctcgacctg
      481 cgtcgcctcg ccgccgagca gctgctcgtc agcggtcacc acgccgaggg ccgcgtcgtc
      541 ctgcaggatg tcctcgccga ggtcggcctc cagctcccgc tgacaccgcg ccgcgccctg
      601 ctctcgctgg tcacgcgccg cctccagctc aagctccacg gcatgcgctt cgcgctgcgt
      661 cgcgaggccg agtgcgaccc cgatcaactg atgctgatcg acatctgcac cgtcgtgtcg
      721 atgggcctga gcatgaacga caacatccgc gccgccggct tccagtcgta cggcacgctg
      781 ctggccctgc gcgccggtga gccggtccgg ctgtcgcgcg cgctcgccat ggagtcgagc
      841 ctcgtcgcgt cctccggcgg ccccgcgcgc acccacgcgc tgcgcctctc gcaggccgcc
      901 ctcgacctcg cacaccaggt caggtcgccc gcctcgatcg ccaccgccct gctgcacgcc
      961 ggcgcctgcg cttacctggc cggcggcgag tggcgccgcg ccgccaagct gtgctgcgag
     1021 agcgagaccc tgttccgcga gaaggtcccc ggcagcacct gggccaagac caccgtgcgc
     1081 cgcttctggc tcggatcgct gatgtacctc ggcgagctcg ccgagctgcg ccgccgcctc
     1141 gccgagttcc tcggcgacgc ccgcgaccgc ggcaacgtct acgcgctcac cgacatgcgc
     1201 gcccgcctca acctcgcctg gctgatcgcc gacgagccgg ccgaggcccg ccgccagctc
     1261 gacgacgcga tgcgcagctg gccgagcgac ggctaccacg tgcagcactt caacgccctg
     1321 ctctcgcagg tccaggccga cctctacgag ggcaacccgg ccgccgcggt cccgaagatc
     1381 gaggccgtgt ggcccgcgct gaccggctcg atgctgatgc ggatccagct cgtacgcacc
     1441 gaggcccatc agctgcgcgc ccgcgccggc ctggcgacac tcgccgcctc gggccgcccg
     1501 acccgcgacg cgatcaaggt gatcgagcgc gaagtggatg cgctcggcaa ggagaagatg
     1561 ccgtggatcg acccgctcgc cgagctgctg cgcgccggcc tgcagcgcgt gcagggccgc
     1621 agcgacgccg ccgtc
//
LOCUS       DASWNJ010001376         1729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_94539, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001376 DASWNJ010000000
VERSION     DASWNJ010001376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94539"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1714)
                     /locus_tag="VGB85_22885"
     CDS             complement(<1..1714)
                     /locus_tag="VGB85_22885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0136954.1"
                     /translation="MDTYLAAFFARSSFEMEYRLRRHDGEYRWMVDSGAPRMLIGAGG
                     GDASEFAGYVGSCIDITPRRWAKQELARVNAQLQAVLDASHQVAIIATDPRGEITLWN
                     RGAEQMFGVRAEAVVGRLCTPLVRGDPAFAARRARLSAEFGRAIDGFEVMALRARLYD
                     HDEQEWTLYRGSGERLTVNLVASPMRGGDGEITGYVALARDITDSKAAQVAMQESAVR
                     VQGALHGSMDAIVMLVAIRDDAGVLIDLMFTDLNPRAEQMLGKAAGMLVGVRMSESYN
                     APGLLAFLGRCEQVIKTGQPLEEEFQLRQGLVPGLDWMRMQVVALGDGVVVTSRDISQ
                     RKRDEEQLRLSEARLNMALEATQDALWDWNFVTGEIYVSPRWLARLGLDPRTPVLPQK
                     EMDARTHPEDVASVREAVLDHIAGRTAAYEREYRVRDAAGEWSWVLARGQVVERDTRG
                     RVKRMLGTASDVTARKHQEAELLRAKEAAERANQAKSEFLANVSHEIRTPMNGILGMV
                     ELALDDDLAQEQRDRLRLVHDSARSLLAIINDLLDLAKIEAGKFVLDPNRFALREEVE
                     QTLRS"
BASE COUNT          227 a          657 c          582 g          263 t
ORIGIN      
        1 gcgagcgcag cgtctgctcg acctcctcgc gcagcgcgaa gcggttcggg tcgagcacga
       61 acttgccggc ctcgatcttc gccaggtcga gcaggtcgtt gatgatcgcc agcagcgagc
      121 gcgccgagtc gtggaccagg cgcagacgat cacgctgctc ctgcgcgagg tcgtcgtcga
      181 gcgccagctc gaccatcccg aggatcccgt tcatcggcgt ccggatctcg tggctcacgt
      241 tggcgaggaa ctcgctcttg gcctggttgg cccgctccgc cgcctccttc gcgcgcagca
      301 gctccgcctc ctggtgcttg cgcgccgtca cgtcgctcgc cgtgccgagc atccgcttca
      361 cgcggcccct ggtgtcgcgc tcgaccacct ggccacgcgc gagcacccac gaccactcgc
      421 cggccgcgtc gcgcacgcga tactcgcgct cgtaggccgc ggtgcgcccg gcgatgtggt
      481 cgagcaccgc ctcgcgcacg ctcgcgacgt cctccgggtg ggtgcgcgcg tccatctctt
      541 tttgcggcag caccggcgta cgcgggtcga ggcccaggcg cgccagccag cgcgggctga
      601 cgtagatctc gccggtgacg aagttccagt cccacagcgc gtcctgggtc gcctcgagcg
      661 ccatgttgag gcgcgcctcg ctgagccgca gctgctcctc gtcgcgcttg cgctgcgaga
      721 tgtcgcgcga cgtgacgacc accccgtcgc cgagcgcgac cacttgcatg cgcatccagt
      781 cgagcccggg cacgaggccc tggcgcagct ggaactcctc ctcgagcggc tgcccggtct
      841 tgatcacctg ctcgcagcgg ccgaggaacg ccagcaggcc gggggcgttg taggactccg
      901 acatgcgcac gccgacgagc atccccgcgg ccttgccgag catctgctcc gcgcgcgggt
      961 tgaggtcggt gaacatcagg tcgatcagca cgccggcgtc gtcgcggatc gccaccagca
     1021 tcacgatcgc gtccatgctg ccgtgcaggg cgccctgcac gcgcacggcg ctctcctgca
     1081 tcgctacctg cgccgccttg ctgtcggtga tgtcgcgggc gagcgcgacg tagccggtga
     1141 tctcgccgtc gccgccgcgc atcggcgagg cgacgaggtt gacggtcagg cgctcgccgc
     1201 tgccgcggta cagcgtccac tcctgctcgt cgtggtcgta gaggcgggcg cgcagcgcca
     1261 tcacctcgaa gccgtcgatc gcgcgcccga actcggcgct caggcgcgcg cgccgggccg
     1321 cgaacgccgg gtcgccgcgg accagcggcg tgcacagccg gccgaccacc gcctccgcgc
     1381 ggaccccgaa catctgctcg gccccgcggt tccacagggt gatctcaccc cgggggtccg
     1441 tcgcgatgat cgccacctga tgcgacgcgt cgagcaccgc ctgcagctgc gcgttgaccc
     1501 gcgcgagctc ctgcttggcc cagcgccgcg gcgtgatgtc gatgcacgac ccgacgtagc
     1561 ccgcgaactc ggaggcgtcg ccgccgccag caccgatgag catccgcggc gccccgctgt
     1621 cgaccatcca ccggtactcg ccgtcgtggc ggcgcaggcg atactccatc tcgaagctgc
     1681 tgcgcgcgaa gaaggccgcg agataggtgt ccatgcaacg ctggcggtc
//
LOCUS       DASWNJ010001377         2806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_376253, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001377 DASWNJ010000000
VERSION     DASWNJ010001377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376253"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /locus_tag="VGB85_22890"
     CDS             complement(<1..1349)
                     /locus_tag="VGB85_22890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0136955.1"
                     /translation="MTSPQQRIKDAMLWGVPYAGSPSLAARIMLTNQMLMLVMATALV
                     YAIIFSVAGPGNEGLLALPFALEFFACMLANRMGWYNVARVGLMFGCGAALAVFASLF
                     GRDSGIQLVLFPIVGFPLICFEPRERWQRWACSGFIIATFFGLELTDYRILRILSASP
                     IDAAVQHAIYYTVILTTFVLTLLPLRLFYLASTRAEERLGRTNAELQRVNDQLNRARD
                     EAVLANQAKNLFLANMSHELRTPLNAIIGYSELVREELEESGVSEASVDLDKIRGAGK
                     YLLNIVSDVLDLSKIEAGRVDMFWENFPLSDLIDDVAAWVRPHAEKRRNRLEVAYPAS
                     ASGSALGSAALGSITADKTRLRQALVNLLQNACKFTENGVVRVAVSREHTSDAEWIVF
                     KITDTGIGMAPETVRELFQPFTRGDLDSMRKYEGTGLGLAISRRLCRMMGGDITVEST
                     "
     gene            complement(1353..2348)
                     /locus_tag="VGB85_22895"
     CDS             complement(1353..2348)
                     /locus_tag="VGB85_22895"
                     /inference="COORDINATES: protein motif:HMM:NF040195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136956.1"
                     /translation="MSFHMHPPAGETVNPHHAEVELHNVAWVQRHSPGMADDDDLLRR
                     LRRTRFSRLAAFTYPRVGAAELALICNWITWLFLHDDCYCDDGDGDEASLTAFHREVL
                     AVLRGEHAPRPGDVSLLHMLVDLRRRMLVWSDAAWMSRFIVSVDRYLQSTRWEAHNRG
                     QGVVPPLAAYVKMRCYTGAMDSVYDCIELGEHLRLGPAIREHSVITTLEQMAGNCVCW
                     ANDIFSVNKELLEHNAHNLVFVLRHEHQLTLADALAKAVAMHDAELHAFEWAAARLPE
                     FHREFGAEKTQQVRRYVAGLRSWVRGNAAWSWETPRYKGCLSMRGPNESQLSPGA"
     gene            2476..>2806
                     /locus_tag="VGB85_22900"
     CDS             2476..>2806
                     /locus_tag="VGB85_22900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136957.1"
                     /translation="MSAAPRTTLASFAALGPLLWFAGMIALAVVLVGGDYEAHGGGLV
                     PPEHHPPAGSHVRVSLTSEAAEEAWPQVVDLLGPTLPGHWPLAPPPAETRAWLDAHLL
                     YRLQPGDH"
BASE COUNT          425 a          940 c         1019 g          422 t
ORIGIN      
        1 gtggattcca ccgtgatgtc gccgcccatc atgcgacaca ggcgccggct gatcgccagg
       61 ccgaggccgg tgccctcgta cttgcgcatg ctgtcgaggt cgccgcgggt gaacggctgg
      121 aacagctcgc gcacggtctc gggcgccata ccgatgcccg tgtcggtgat cttgaacacg
      181 atccactcgg cgtcggaggt gtgctcgcgg ctgaccgcga cgcgcacgac cccattttcg
      241 gtgaacttgc aggcgttctg aagcaaattg acgagcgcct ggcgcaggcg ggtcttgtcg
      301 gcggtgatgc tgccgagggc ggcgctgccg agggcgctgc cggaggcgga ggccgggtag
      361 gcgacctcga ggcggttgcg tcgcttctcg gcgtgcggcc ggacccaggc ggcgacgtcg
      421 tcgatgaggt cggagagcgg gaagttctcc cagaacatgt cgacgcggcc ggcctcgatc
      481 ttcgacaggt cgagcacgtc gctgacgatg ttgagcaggt acttgccggc gccgcggatc
      541 ttgtcgaggt cgacgctcgc ctcgctgacg cccgactcct cgagctcctc gcgcaccagc
      601 tcgctgtagc cgatgatcgc gttgagcggc gtgcgcagct cgtggctcat gttggcgagg
      661 aacaggttct tggcctggtt ggcgagcacg gcctcgtcgc gggcgcggtt gagctggtcg
      721 ttgacgcgct gcagctcggc gttggtgcga ccgaggcgct cctcggcgcg cgtcgaggcg
      781 aggtagaaca ggcgcagcgg cagcagggtc agcacgaagg tcgtcaggat gacggtgtag
      841 tagatcgcgt gctggaccgc ggcgtcgatc gggctcgcgc tcaggatccg caggatgcgg
      901 tagtccgtca gctcgaggcc gaagaaggtc gcgatgatga agccgctgca ggcccaccgc
      961 tgccagcgct cgcgcggctc gaagcagatc agcgggaagc cgacgatcgg gaacagcacc
     1021 agctggatcc ccgagtcgcg gccgaacagc gaggcgaaca ccgccagcgc ggcgccgcag
     1081 ccgaacatca ggccgacgcg ggcgacgttg taccagccca tgcggttggc gagcatgcac
     1141 gcgaagaact cgagcgcgaa gggcagcgcg agcaggccct cgttgccggg cccggcgacg
     1201 ctgaagatga tcgcgtagac gagcgcggtc gccatcacca gcatcagcat ctgattcgtc
     1261 agcatgatgc gggccgcgag gctgggcgag ccggcgtacg gcacacccca gagcatcgcg
     1321 tccttgatcc gttgctgcgg cgaggtcacc actcacgctc ccggcgacag ctgtgactcg
     1381 ttggggccgc gcatgctcag gcagcccttg tagcgcggcg tctcccacga ccacgccgcg
     1441 ttgccgcgga cccacgagcg caggccggcg acgtagcggc ggacctgctg ggtcttctcc
     1501 gcgccgaact cgcggtgaaa ctcggggagg cgggcggcgg cccactcgaa ggcgtggagc
     1561 tcggcgtcgt gcatggcgac ggccttggcg agcgcgtcgg cgagggtgag ctggtgctcg
     1621 tggcggagca cgaacacgag gttgtgggcg ttgtgctcga gcagctcctt gttgacggag
     1681 aagatgtcgt tggcccagca cacgcagttg ccggccatct gttcgagggt ggtgatcacg
     1741 ctgtgctcgc ggatggcggg gccgaggcgc aggtgctcgc cgagctcgat gcagtcatac
     1801 accgagtcca tcgcgccggt gtagcagcgc atcttgacat aggcggcgag cggcggcacg
     1861 accccttgcc cgcggttatg cgcctcccag cgggtgctct ggaggtagcg gtcgacgctg
     1921 acgatgaagc ggctcatcca cgcggcgtcg gaccacacga gcatgcgccg gcgcaggtcg
     1981 acgagcatgt gcagcagcga gacatcgccg ggtcgcggcg cgtgctcgcc acggaggacc
     2041 gcgagcacct cgcggtggaa cgcggtcagg ctggcctcgt cgccgtcgcc gtcgtcgcag
     2101 tagcagtcgt cgtgcaggaa cagccaggtg atccagttgc agatcagcgc cagctcggcg
     2161 gcgccgaccc gcgggtacgt aaaggccgcg agccgcgaga accgggtccg tcgcaggcgt
     2221 cgcaggaggt cgtcgtcgtc ggccatcccg gggctgtgac gctgcaccca cgcgacgttg
     2281 tgcagctcga cctccgcgtg gtgcggattg accgtctcac ccgccggtgg atgcatgtga
     2341 aaggacatcg ccgaccagag agccgccgtt acccaacacc catacccagc agccgggcgc
     2401 ggtgcaagcg acccgcgccg cggacacgtg tcgcccgcgg taccctgccg gggccttgtg
     2461 gtatggtccg ccggcttgtc ggccgcgccc cgcacgacgc tcgcctcgtt cgccgcgctc
     2521 ggaccgctgc tgtggttcgc cggaatgatc gctctggcgg tcgtgctcgt cgggggcgac
     2581 tacgaggcgc acggcggcgg gctcgtgccc cccgaacacc acccgcccgc cggcagccac
     2641 gtgcgcgtca gcctcaccag cgaggccgcc gaggaggcgt ggcctcaggt cgtcgacctg
     2701 ctggggccca ccctccccgg acactggccg ctcgccccgc cacccgccga gacgcgcgcc
     2761 tggctcgacg cccacctgct ctaccgcctg caaccgggcg accacg
//
LOCUS       DASWNJ010001378         2457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_556040, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001378 DASWNJ010000000
VERSION     DASWNJ010001378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556040"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VGB85_22905"
     CDS             complement(<1..523)
                     /locus_tag="VGB85_22905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136958.1"
                     /translation="MIDALLSRLRSATPELNLAAVEEEYRDPFWWDRYGERGHRFAVE
                     DGQHHFSYVAEAVAGGSPQLLARYARWLRGVLVARGMCSEHLADGFRIRARVLAARGW
                     PHVDLALAAFTAAEDALRYTEGPAAGLVPAPGDPPPGSDVAAAFAVPADEVAHDARTL
                     LSYLADALALANPQ"
     gene            complement(520..1173)
                     /locus_tag="VGB85_22910"
     CDS             complement(520..1173)
                     /locus_tag="VGB85_22910"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEY0136959.1"
                     /translation="MPRELAAIRTEYLEAQLAGDQRAALRVVREALADGHPVRALQGE
                     VVQAAQLEIGRLWQENRISVAHEHMATAISQVALVHLIEHGEMAQARERKIVVACVEG
                     EHHDLPARLVADYLELAGFDIRFLGGNVPSDSLASVVAIERPDLLALSMTMSFNARGL
                     ESAVTSVRARCPGLPILVGGHALDWLPELAKKLDVTAVSGSPDAIVAAVERTLESAK"
     gene            complement(1352..>2457)
                     /locus_tag="VGB85_22915"
     CDS             complement(1352..>2457)
                     /locus_tag="VGB85_22915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0136960.1"
                     /translation="LGPELARRMGIRAIVRPAGENFGNRRAAVALDLPTPTVADTPPG
                     PADARLPAGLWRIEATAPGFLPSTREFTVDATTPEQTLRWNLLPDSAHDDVRFVVSAT
                     GTPQVSVRVRSADGARTWTCLSQRSACVLRLTPGEWHAEASAPGFRTTRRSFTVVRGA
                     PSEVTLELVAGSDDLASATGPKGQIPANLRKRLALGLGLSAAPVFAAGIGLTLTGRLQ
                     YVSALHGDACEGSYRVGCGDAAIGPSHRASAGVGLLGAAAGLIATGLTGLYPVPRTAW
                     YVELGLGGALTVAGIGWITANSAALERAHASGPITELAARADRRLAPSVFIGLGVGTL
                     AGALTGLLVQRKAGRVAPYAGSGQAGLLWYGRF"
BASE COUNT          320 a          803 c         1032 g          302 t
ORIGIN      
        1 cctgcgggtt ggcgagggcg agggcgtcgg cgaggtagga gagcagggtg cgggcgtcgt
       61 gcgcgacctc gtcggcgggc acggcgaagg ccgcggcgac gtcgctgcct ggcggcgggt
      121 cgccgggggc cggcacgagg ccggcggcgg ggccttcggt gtatcgcagg gcgtcctcgg
      181 cggcggtgaa ggcggcgagg gcgaggtcga cgtgcggcca gccgcgggcg gcgaggaccc
      241 gggcccggat gcgaaagccg tcggcgaggt gctcgctgca catgccgcgc gcgacgagca
      301 cgccgcgcag ccagcgggcg tagcgggcga gcagctgcgg gctgccgccg gcgacggcct
      361 cggcgacgta gctgaagtga tgctggccgt cctcgacggc gaagcggtgg ccgcgttcgc
      421 cgtagcggtc ccaccagaac gggtcgcggt actcctcctc gacggcggcg aggttgagct
      481 ccggcgtggc gctgcgcagc ctggagagca gtgcatcgat cacttcgcgg actccagggt
      541 gcgctcgacg gcggcgacga tcgcgtcggg gctgccgctg acggcggtga catcgagctt
      601 tttcgcgagc tcgggcaacc agtcgagggc gtgaccaccg acgaggatcg gcaagccggg
      661 gcagcgcgcg cgcacgctgg tgaccgcgga ctcgaggcca cgcgcgttga aggacatcgt
      721 catcgacagg gcgagcaggt ccggccgctc gatcgccacg accgaggcga ggctgtcgct
      781 cggcacgttg ccgccgagga agcggatgtc gaagccggcg agctcgaggt aatccgcgac
      841 gagccgcgcg ggcaggtcgt ggtgctcgcc ctcgacgcag gcgacgacga tcttgcgctc
      901 gcgcgcctgc gccatctcgc cgtgctcgat caggtggacc agcgcgacct gcgagatcgc
      961 ggtcgccatg tgttcgtgcg cgacgctgat gcggttctcc tgccacagcc gcccgatctc
     1021 cagctgcgcg gcctggacga cctcgccctg cagcgcccgc accgggtggc cgtccgcgag
     1081 cgcctcgcgg acgacccgca gcgcggcccg ctgatcaccc gcgagctgag cctcgaggta
     1141 ttcggtgcgg atcgctgcga gctcgcgggg catggatcca caatgtgtct cgaggccgcg
     1201 acccggccaa aacggtcgcg tgacgcgctg cacgcccggc ctcgtcctcg ctggactggc
     1261 agggatgctc gagacgaggg gtcttcccag gtcgggtggc tttcgctcgg ccgcacaaag
     1321 tgcgcaccgg gcgccttgca aggtccagcg atcagaagcg gccgtaccac agcaggccgg
     1381 cctggccgct gccggcgtag ggggcgacgc ggccggcctt gcgctggacg aggaggccag
     1441 tgagggcgcc ggcgagggtg ccgacgccga gcccgataaa gacgctgggc gcgaggcgcc
     1501 ggtcggcgcg ggccgccagc tcggtgatcg ggccggaggc gtgggcccgc tcgagggcgg
     1561 ccgagttggc ggtgatccat ccgatccccg cgaccgtcag cgcgccgccg aggccgagct
     1621 cgacgtacca ggcggtccgc gggaccgggt agaggccggt gaggccggtg gcgatcaggc
     1681 cggcggcggc gccgagcagg ccgacgccgg cgctggcgcg gtggctgggg ccgatcgcag
     1741 cgtcgccgca gccgacccgg tagctgccct cgcaggcgtc gccgtgcagg gcgctgacgt
     1801 attgcaggcg gccggtcagg gtcaggccga tgccggcggc gaacaccggg gccgcggaca
     1861 ggccgaggcc gagcgcgagg cgcttgcgga ggttggccgg gatctggcct ttgggacctg
     1921 tcgcggaggc caggtcgtcg ctgccggcga cgagctcgag ggtgacctcg gacggggcgc
     1981 ccctgaccac ggtgaagctg cgccgggtgg tgcgaaagcc gggcgcgctg gcctcggcgt
     2041 gccactcgcc gggggtgagg cgcaggacgc aggcggagcg ctggctgagg caggtccagg
     2101 tccgggcgcc gtcggcagat cgcacgcgga cgctgacctg cggggtgccg gtggcggaca
     2161 cgacgaagcg gacatcgtcg tgggcgctgt cggggagcag gttccagcgc agggtctgct
     2221 cgggggtggt cgcgtcgacg gtgaactcgc gggtgctcgg gaggaagccg ggagcggtgg
     2281 cctcgatgcg ccacaggccg gcgggcaggc gggcgtcggc ggggccgggc ggcgtgtcgg
     2341 cgacggtcgg cgtcgggagg tcgagggcga cggcggcgcg gcggttgccg aagttctcac
     2401 cggccggccg aacgatcgcg cggatgccca tgcggcgagc gagctcgggg ccgaggt
//
LOCUS       DASWNJ010001379         3987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_556102, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001379 DASWNJ010000000
VERSION     DASWNJ010001379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556102"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VGB85_22920"
     CDS             <1..401
                     /locus_tag="VGB85_22920"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY0136961.1"
                     /translation="CGFASRNARLAAALMAEAAEKVPPAVLEDARAAATLMAMNNIYY
                     RTKHMLGREEYQTLPARLRMTRIAKPATNKLDFELMCLAVSAINGCERCIQAHEKTVR
                     DAGMPAEKVHDAIRIAATIQGVAVGLELGT"
     gene            678..1655
                     /locus_tag="VGB85_22925"
     CDS             678..1655
                     /locus_tag="VGB85_22925"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0136962.1"
                     /translation="MSNQDQSASTRSSITTGITPASQSQYEGYQGTELPPGTVVSCSS
                     QFEIVQRLGEGGMSKVYKAFDRLMNRYVALKVMKNDVPDWAHRHFRREARMCASFMHN
                     NLVHVMQVGTTEEYDLLWFAMEYLRGKDVGWFVDSGTSMGIPVICEIFSQVLDALRYV
                     HLRRIVHCDIKPSNIFVTRDSHDPLLRVVKLLDFGVARDLSDLSSHSTGHVMGDPRYI
                     APEQTIPGNYIDHRADIYALGMTFFELVTGGRHPFEDLFDEHPRQLLVAHREREPLPV
                     SHFLSESVPAAKARAIDAFFEKSCGKQAHHRFADAAQMKEALSELAKFG"
     gene            1660..2637
                     /locus_tag="VGB85_22930"
     CDS             1660..2637
                     /locus_tag="VGB85_22930"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY0136963.1"
                     /translation="MTTPVLAGDDPAAIARAAQVLRDGRLVAFPTETVYGLGGDALDA
                     AVITKIFAAKQRPADNPLIVHVAEVAGARALVREFPEVAEKAATLWPGPLTIVLRRND
                     RVPDATTAGQDTVALRVPDHPVALALLRACGLPLAAPSANRSGRPSPTLASHVLADLD
                     GRVDLILDGGPTRHGLESTVLDLTGPRPLLLRRGAVTLEQLRALLGEVDSVDEADEAA
                     KARSPGLRHRHYAPRARVELVSEGTGEAAAARAIAGGEQVALVCRREVRSAALVERLP
                     AGLPEFARALFASLRALDATGVDRIVVEAVPEDGIGAAIMDRLRRAAAG"
     gene            2682..>3987
                     /locus_tag="VGB85_22935"
     CDS             2682..>3987
                     /locus_tag="VGB85_22935"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0136964.1"
                     /translation="MAEPDDPVNAPGLRLGALLGSGAVATVARVESNDGRVFAGKVLH
                     ASHGQDSAATQRFAQEAALLRGVVDPNIVTVFGVAEIEGKQVLLLELVEGPTLAQLIA
                     REAPLAESRLARLAAGVARGLARAHAAGVIHRDLKPANILVAAGDVPKIADFGMARAT
                     SLAGVERSALAVLGTPDYMAPESLDPLAVDTRSDLYALGCILFEMATGRPPHTGATAF
                     GVIEAHRKAITPALGEGFSPPLRALVASLLAKSPADRPQAATQVAALLEQIADGHVTT
                     LAVVAEHTPGGPACAGCGQPLVPELAVCMHCGLAVARAEPGRWTVLVAGPGEPGDKLD
                     SALRARLIGWIAGNPGLGVVAGSLEKTIPRVPFTLLTRVSEASGRSIVAALARLGLEA
                     EVVQGGALRSVRMRKKAGALIGRTLAVAFASSAGLMSNKYILF"
BASE COUNT          568 a         1408 c         1460 g          551 t
ORIGIN      
        1 cctgcgggtt cgcgtcgcgc aacgcgcgac tggccgcggc cctgatggcc gaggccgccg
       61 agaaggtccc gcccgccgtc ctcgaggacg cgcgggcggc cgccacgctg atggcgatga
      121 acaatattta ttaccgcacc aagcacatgc tcgggcgcga ggagtaccag acgctgccgg
      181 cgcgcctgcg gatgacccgc atcgccaagc cggcgaccaa caagctggac ttcgagctga
      241 tgtgcctggc ggtcagcgcc atcaacggct gcgagcgctg cattcaggcc cacgagaaga
      301 ccgtgcggga cgctggaatg ccggccgaga aggtccatga cgcgatccgc atcgcggcga
      361 ccatccaggg cgtcgcggtc gggctcgagc tcgggacctg agagccccgc cacatcgaca
      421 cgcgccagcc cgcgcatccg cggcggtagg cgcggcgtcg cgcaatcgta gacgagtggc
      481 ggcacggacg ccccggaaca gggttcgccc tggaccgggg cgcgcgtcgt tccgcttcgc
      541 cgcgccgcga cccgcagacc gcgtcagatg cagggaagct gacgagatgg gtacaggtgg
      601 tgtagcttgc gtagcctgcc tcacggcagt tacaccgccg cgcgtcgcgg cacccacttt
      661 cgggtctgta ccctcacgtg tcgaatcagg accaaagcgc cagcacccgt agctcgatca
      721 cgaccggcat cacgccggcg tcgcagtcgc agtacgaggg ctatcagggc accgagctgc
      781 cgcctggcac ggtggtctcg tgctcgtcgc agttcgagat cgtgcagcgg ctcggcgagg
      841 gcgggatgag caaggtctac aaggccttcg accgcctgat gaaccgctac gtcgcgctca
      901 aggtgatgaa gaacgacgtg cccgactggg cccaccggca ctttcgccgc gaggcccgga
      961 tgtgcgcgag cttcatgcac aacaacctcg tgcacgtgat gcaggtcggc accaccgagg
     1021 agtacgacct gctgtggttc gcgatggagt acctgcgcgg caaggacgtc ggctggttcg
     1081 tcgacagcgg cacgtcgatg ggcatcccgg tgatctgcga gatcttctcg caggtgctcg
     1141 atgcgctgcg ttacgtgcat ctgcgcagaa ttgtccactg cgacatcaag ccgtcgaaca
     1201 tcttcgtcac ccgcgactcg cacgacccgc tgctgcgcgt cgtcaagctg ctcgacttcg
     1261 gcgtcgcgcg tgacctcagc gacctctcgt cgcacagcac cggccacgtg atgggcgacc
     1321 cccgctacat cgcgccggag cagacgatcc ccggcaacta catcgaccac cgcgccgaca
     1381 tctacgcgct cggcatgacc ttcttcgagc tggtcaccgg cggccgccat cccttcgaag
     1441 acctgttcga cgagcacccg cggcaattgc tggtcgcgca ccgcgagcgt gaaccattgc
     1501 cggtgtcgca ctttttgtcc gagtcggtgc cggccgccaa ggcgcgggcg atcgacgcgt
     1561 tcttcgagaa gagctgcggc aagcaggcgc accaccgctt cgccgacgcg gcccaaatga
     1621 aggaagcgct cagcgagctg gcgaagttcg ggtgagcgcg tgacgacgcc agtgctcgcg
     1681 ggcgatgacc ccgccgcgat cgcaagggcc gcgcaggtac tgcgagacgg caggctggtc
     1741 gccttcccga ccgagacggt gtacgggctc ggcggcgacg cgctcgacgc ggcggtgatc
     1801 acgaagatct tcgcggcgaa gcaaagaccg gcggacaatc cgctgatcgt gcacgtcgcc
     1861 gaggtcgcgg gggcaagagc gctggtccgc gagttcccgg aggtcgcgga gaaggccgcg
     1921 acgctgtggc cggggccgct gacgatcgtg ctgcggcgca acgacagggt gcccgacgcg
     1981 acgacggcgg ggcaggacac ggtggcgctg cgggtgcccg atcatccggt cgcgctcgcg
     2041 ctgctgcgcg cctgcgggct gcccctcgcg gccccctcgg ccaatcgctc cgggcggccg
     2101 agcccgacgc tggcgagcca cgtgctcgcg gacctcgacg ggcgcgtcga cctgatcctc
     2161 gacggcggcc cgacgcggca cgggctcgag tcgacggtgc tcgacctgac cgggccgcgg
     2221 ccgctgctgc ttcgccgcgg tgcggtgacg ctcgagcaac tgcgcgcctt gctcggcgag
     2281 gtcgacagcg tcgacgaggc cgacgaggcc gcgaaggcgc gctcgccggg actgcggcac
     2341 cggcactacg cgccgcgggc ccgggtcgag ctggtgtccg aggggacagg tgaggccgcg
     2401 gcggcgcggg cgatcgcggg cggcgagcag gtcgcgctgg tgtgccgcag ggaggtgcgc
     2461 agcgcggcgc tcgtcgagcg actgccggcc gggctcccgg agttcgcgcg ggccctgttc
     2521 gccagcctgc gcgcgctcga tgccacgggg gtcgatcgca tcgtcgtcga ggcggtgccc
     2581 gaggacggca tcggcgcggc gatcatggat cgcctgcggc gcgcggcggc ggggtagcag
     2641 cgggctcgcg aaagcggccg cgatcgggca tgatgcgccg cgtggccgag cccgacgatc
     2701 ccgtgaacgc gccgggcctg cggctcggcg cgctgctcgg cagcggggcg gtcgccacgg
     2761 tcgcgcgcgt cgagtcgaac gacggccggg tgttcgccgg caaggtcctg cacgcgtcgc
     2821 acggccagga cagcgcggcg acccagcgct tcgcgcagga ggccgcgctg ctacgcgggg
     2881 tcgtcgaccc caacatcgtc acggtgttcg gtgtcgccga gatcgaaggc aagcaggtgc
     2941 tgctgctcga gctcgtcgag ggcccgacgc tcgcccagct gatcgcccgc gaggcgccgc
     3001 tcgccgagtc acggctggcg aggctcgccg cgggcgtcgc tcgtgggctc gcgcgggcgc
     3061 acgcggcggg cgtgatccat cgcgacctca agccggccaa catcctcgtc gcggccggcg
     3121 atgtcccgaa gatcgcggac ttcgggatgg cccgcgcgac gtccctcgcc ggcgtcgagc
     3181 gctcggcgct ggcggtgctc ggcacgcccg actacatggc gcccgagtcg ctcgacccgc
     3241 tcgccgtcga cacccgcagc gacctctacg cgctcggctg cattctcttc gagatggcca
     3301 ccggccgccc gccgcacacc ggcgcgaccg ctttcggcgt gatcgaggcc caccgcaagg
     3361 cgatcacgcc ggcgctcggc gagggcttct cgccgccgct gcgggcgctg gtcgccagct
     3421 tgctcgccaa gtcgccggcg gaccgcccgc aggcggcgac gcaggtcgcg gcgctgctcg
     3481 agcagatcgc ggacgggcac gtcacgacgc tcgcggtggt cgccgagcac acgccgggtg
     3541 gaccggcctg tgcgggctgc gggcagccgc tggtgcccga gctggcggtg tgcatgcact
     3601 gcggcctcgc ggtcgcgcgc gcggagcccg gtcgctggac ggtgctcgtc gctggccccg
     3661 gcgagcccgg cgacaagctg gacagcgccc tgcgagcgcg gctgatcggc tggatcgccg
     3721 gtaatcccgg gctcggcgtc gtcgcgggct cgctcgagaa gacgatcccc cgcgtgccct
     3781 tcacgctgct gacgcgcgtc tcggaggcga gcggtcgcag catcgtcgcc gcgctcgcgc
     3841 gcctcggtct cgaggccgag gtcgtgcagg gcggggcgct ccggtcggtg cgcatgcgca
     3901 agaaggccgg ggcgctgatc ggccgcacgc tcgcggtcgc gttcgcctcg agcgccgggc
     3961 tgatgagcaa caagtacatc ctgttcg
//
LOCUS       DASWNJ010001380         2070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_201796, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001380 DASWNJ010000000
VERSION     DASWNJ010001380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201796"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1427
                     /locus_tag="VGB85_22940"
     CDS             135..1427
                     /locus_tag="VGB85_22940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136965.1"
                     /translation="MKRSLFSAGLLALACQAPAAPPAAILAAPAPAPTVPPVLRHADE
                     LARIDEKIAAVLARADAQPRGWLALEQAANLYLTRARLSGDYDDYARAEALVERAFAV
                     AGDGVGPFFTRVGLDFTLHRLDRVARDLDTIAGFAIVSDEDRRTLVALRADLAFQQGR
                     YTEAGRGFEQALAAHEDVNSLAHLALYRWKTGDFLAAEDLYHRALAKLRPGAGEPLAW
                     LHLQLGLMDLERGRLDEALAHYHDGAAELGGYWLIDEHIAEILTLQGKTDEALAAYAD
                     ILRRTDNPEFMDAVAAIHLAAGRTDEALRMITRAREIYEAQLRRFPEAAHGHALDHFL
                     ELGDDPARTLVIAEANHRTRPNAEAKISLARAYLEAGRVDAARTILAEALATPWNTAD
                     LHAAAAEVFVAAGDVAAADRARARALAIDPHVLDSPGR"
     gene            1459..1797
                     /locus_tag="VGB85_22945"
     CDS             1459..1797
                     /locus_tag="VGB85_22945"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEY0136966.1"
                     /translation="MKRSVQVEIAGQQLTIRSDEGAEYVHQLAEYVDAHLRVLSGGRR
                     ITNTSGLQRVALLVAMQLADELFREKDLHQRFRRRVNDRLLGLRRALDEHERRLAALD
                     EPPKDTPAAL"
     gene            1865..>2070
                     /locus_tag="VGB85_22950"
     CDS             1865..>2070
                     /locus_tag="VGB85_22950"
                     /inference="COORDINATES: protein motif:HMM:NF012788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136967.1"
                     /translation="MTASPALSARSPALATFLERGYFEQASDFAAIDADMASGTVTFY
                     VGYDPTGASLHVGHLVQIMAMRAL"
BASE COUNT          278 a          849 c          657 g          286 t
ORIGIN      
        1 gccaagctcc cgctcaaccc gacgaagaac gacaaggagc tcctcccgga cttcccctac
       61 gtcgccgcgc cgcactagtc gccggcgtcg ctcctccgcg gcccgcccgg ccatctcgtc
      121 ggatccgtat cacgatgaag cgatccctct tctcggccgg cctgctcgcg ctcgcctgcc
      181 aggcgcctgc cgccccgccc gctgcgatcc tcgccgcgcc cgcgcccgcg cccaccgtgc
      241 cgccggtgtt gcgccacgcc gacgagctcg cgcgcatcga cgaaaagatc gccgccgtgc
      301 tcgcgcgcgc cgatgcccag ccgcgcggat ggctcgcgct cgagcaggcc gcgaacctgt
      361 acctcacgcg cgcccgcctc agcggcgatt acgacgacta tgcgagggcc gaggcgctcg
      421 tcgagcgcgc cttcgcggtc gcgggcgacg gcgtcggtcc gttctttacc cgcgtcggcc
      481 tcgacttcac gctgcatcgc ctcgaccgcg tcgcccggga cctcgacacg atcgccggct
      541 ttgcgatcgt cagcgacgag gaccggcgca cgctcgtggc cctgcgcgcc gacctggcct
      601 ttcagcaagg tcgctacacc gaggccgggc ggggcttcga gcaggcgctc gcggcccacg
      661 aggatgtcaa cagcctcgcg cacctcgccc tgtaccgctg gaagaccggc gactttttgg
      721 ccgccgagga cctctatcac cgggcgctcg ccaaactgcg acccggcgcc ggtgagccgc
      781 tcgcgtggct gcacctccag ctcggcctga tggacctcga gcgcggccgc ctcgacgagg
      841 cgctcgcgca ctaccacgac ggcgcggccg agctcggcgg ttactggctg atcgacgagc
      901 acatcgccga gatcctgacc ttgcagggca agaccgacga ggcgctcgcg gcctacgccg
      961 acatcctgcg ccgcaccgac aaccccgagt tcatggacgc cgtcgccgcc atccacctcg
     1021 ccgccggtcg cacggacgag gcgctgcgca tgatcaccag ggcccgcgag atctacgagg
     1081 cccagctgcg gcgcttcccg gaggccgccc acggccacgc gctcgatcac ttcctcgagc
     1141 tcggcgacga ccctgcccgt acgctggtga tcgccgaggc caaccaccgc acccgtccca
     1201 acgccgaggc gaagatctcg ctggcccgcg cgtacctcga ggccgggcgc gtcgacgcgg
     1261 cgcggacgat cctcgccgag gcgctggcga ccccgtggaa caccgccgac ctgcacgccg
     1321 ctgccgccga ggtgttcgtc gccgcgggcg acgtcgcggc cgccgacagg gcccgtgccc
     1381 gggctctggc gatcgacccc cacgtgctcg actcgcccgg ccggtgacag cggcgcgcgc
     1441 ccgcgctatc gtgccggcgt gaaacggtcg gttcaggtcg agatcgccgg gcagcagctc
     1501 accattcgca gcgacgaggg cgccgagtac gtccaccagc tcgccgagta cgtcgacgcc
     1561 cacctgcgcg tgctgtccgg tggccgccgg atcaccaaca ccagcggcct ccagcgcgtc
     1621 gcgctgctgg tcgccatgca gctcgccgac gagctgttcc gcgagaagga cctccaccag
     1681 cggttccgcc ggcgcgtcaa cgaccgcctg ctcggcctgc gtcgcgccct cgacgagcac
     1741 gagcgccgcc tcgccgccct cgacgagccg cccaaggaca cgcccgccgc cctctgaggc
     1801 gctcccgctc gctgtttcgg ccggtggcca gcgggccgcc ggactgcgct atccttcgcc
     1861 ggtcatgacc gcctcgcctg ccctttcagc gcgctcccct gccctcgcca ccttcctcga
     1921 gcgcgggtac ttcgagcagg cctccgactt cgcggcgatc gacgccgaca tggcctcggg
     1981 cacggtcacc ttttacgtcg gctacgaccc gaccggcgcc tcgctgcacg tcggtcacct
     2041 ggtccagatc atggcgatgc gcgcgctgca
//
LOCUS       DASWNJ010001381        10018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_446431, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001381 DASWNJ010000000
VERSION     DASWNJ010001381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 10018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10018
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446431"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..1771
                     /locus_tag="VGB85_22955"
     CDS             92..1771
                     /locus_tag="VGB85_22955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136968.1"
                     /translation="MKRLRPCGLATPVYLFSLLGACGDDQTTTTSASTTTGTTNETST
                     TTGTPTSGASTSSSTSSTSSSSSSSSSSPGDPTDEPPLEIAHGVRLTRVTATQGVQTE
                     LVRDGVEVPAEEYAVPLISRRKTVLRADWLLHAGFTARPLIGRLTVWTPEGDVHVDTS
                     EVTVDGPSNDGDMLKTFAWQLPPALVRPGMEYRIEAFEADPTLATGDISDPPPILPLA
                     GRGALIVEDHPMQIRVELIPIKHVFAGQTCMPEITDADVTAMRQWMEQHNPVERAELF
                     VGEPLEYTASIGTAQDGFVPLLTELGKRRALVKPPPDLYYYGLITSCDAYPPGLVGQA
                     YGIPDAPTMNFAFQRIATGRYLGSGAAARDTFVHEIGHTQGRYHVRCSGGEGGWDPDY
                     PYPNGRIGVWGYGIHDTLLRSPTGFRDYMSYCNTSFVSDYGWKHTYNYIKELSSWDAA
                     SVASDEPILLATIGENGDMSGSGHATWWTTRGQVPQRGRSPGTRVEFTAGGQVYTLSA
                     SIIEIPDSHAHAVAVALPARWTEVSELRLRSAGRPDLVTTRAAVTELHARP"
     gene            complement(1888..2589)
                     /locus_tag="VGB85_22960"
     CDS             complement(1888..2589)
                     /locus_tag="VGB85_22960"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0136969.1"
                     /translation="MTSSEIKLYLPECSTVRVVESSPASWMIQLALEEKDLPYQVKLL
                     PPTTGKDASRELLTRNPRGTFPVLTDGSIVVSEPFAIIEYLEYAHPDPPLLPGTNALR
                     ALALTRLHEAHNLSEVCGQIYDYLSKTKGKDLEPSRVYALADALHDELFFWEYYYGVS
                     AWAAGSELTFADLAVYVHLATAVHLGLGLGEDYPHLHALYQRMRNRPSVDHTWPTSWK
                     PTPYTFLQPAEPDDP"
     gene            2729..4105
                     /locus_tag="VGB85_22965"
     CDS             2729..4105
                     /locus_tag="VGB85_22965"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0136970.1"
                     /translation="MSEPTTSQTPRRVVIVGGGFGGLCAGMGLAGARGVEVTVIDRRN
                     HHLFQPLLYQVAMAGLSPAEIAYPIRTIFSGKRNIRVLLARATGVDLQRKMLRTDDTE
                     IPYDNLILACGARHSYFGHNEWETYAPGLKSLEEATEIRRRVLTAFELAEKEPDPDKQ
                     RKLLTFVVVGGGPTGVELAGALGEISRFTLSKDFRRIDPRRTRVILIEAAPRILAAFD
                     ETLANRATADLESLGVTVWTSTKVTRIDEDGVDLGEETVQAATLLWAAGVAPSELNKS
                     LGVPLDRGGRVIVEKDCSLKDHPEVFVLGDQANFTGEDGKPLPGLAPVAMQQGRYVAR
                     LLLGELRGKPRVPFKYNDKGIMATIGRRRAIVQYGRLKFAGFFAWLAWLFIHVLYLIG
                     FKNRIEVMLNWGWNYFAFSRGARLIVGKDWRAAANSPVPNAVVDMPSAPHPMAGPQTP
                     AAPPAPAA"
     gene            4496..5287
                     /locus_tag="VGB85_22970"
     CDS             4496..5287
                     /locus_tag="VGB85_22970"
                     /inference="COORDINATES: protein motif:HMM:NF021998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HEY0136971.1"
                     /translation="MSSLESSSPLPMDAAPHSEADAARLDMSGSLYMRLVDGGSMTVF
                     FLLEYLLAIEVYQGFYKLDATAHAGWLVPVAFMAGYITADFASGFFHFLADNYGSTTR
                     PFIGPVFIRPFREHHIDPRAITRHDFLEVNGANCAMSVPVLLATWMFLPVGANLVTLF
                     LGAYVGLFLFGIFLTNQFHSWAHVERPPRIVRALQRSGLILGPEHHQRHHTAPFNTYY
                     CITSGWLNPLLARSRFFERIHEPLRRVLEPLFGVADEVGATAPTE"
     gene            complement(5288..5821)
                     /locus_tag="VGB85_22975"
     CDS             complement(5288..5821)
                     /locus_tag="VGB85_22975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136972.1"
                     /translation="MRRGPLAWWLTLCLGVTVPPGAVGGCEATEPAVAPGDPWPSGLA
                     KPKDAAEAASLRAAGVAWTDRQVRQLYLERVAEIGPHDAALRAQGQTAEARARAAFQA
                     RHDARVTARAMMQDSAAVEALRARDQEKYGTPDGPSFEYLVERAQQKGLAGDAVYESI
                     VESAQRTDAATNRSLGL"
     gene            5967..6881
                     /locus_tag="VGB85_22980"
     CDS             5967..6881
                     /locus_tag="VGB85_22980"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEY0136973.1"
                     /translation="MSRLPLLLCCALLGLVPGCAKRADYESSAPSPESASMGGGGDGA
                     TLTDVAAPGPMPASYDGDFATKSTGDYEARDEMAPTSVQAAAARGTPTGGVAADTGKL
                     AEPRPVTPESREGKGTAAQEDDKKTYARQIIYTAEMQISVYKLDDAMQRAEELTLAAG
                     GYIQNLSQGYYVLRIPAPALRGVMDDLARLGVVEARTLQASDVTEQYVDLTTRIKVLR
                     ETQAQLLSLLKQARNVEEALKVRASVDAVTMELEQAMGRLRMLESLIGFSTLTVRMSQ
                     RGPQNTVPSSNDPFPWVDGLGVEATEWN"
     gene            6883..7479
                     /locus_tag="VGB85_22985"
     CDS             6883..7479
                     /locus_tag="VGB85_22985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136974.1"
                     /translation="MTYFKTCSLGLVAAAVLSGCAAYKIDPPVGFAAVSEHDYETRMK
                     AQDNVGLSVRRFENVKGGTLAFWGADLVKKLGDRGYMLRNQSAIKTKNGKTGTRFDFD
                     YETFDTEIPKFYTAILVVTDKYKVVLQIAGGKEFASTYEARVPEIVGQLKVRGCKLGS
                     KICGGPQPPRLSTPKPPAKKVATDEPAEPAASPTAPKP"
     gene            7582..8718
                     /locus_tag="VGB85_22990"
     CDS             7582..8718
                     /locus_tag="VGB85_22990"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY0136975.1"
                     /translation="MHDSAAPSEPAPSPPPRAPGPLDHVDFAAFLADIQALRRELDDS
                     LEEADFKHLQKMERWGRAATAVGLLTCWIGPNPLSAAALGLGRSTRWLLMHHVGHRGY
                     DKVPGVPPRYTSKVFARGARRWLDWLDWIVPEAWIYEHNVLHHSHTGEERDPDLVERN
                     TEALRAKNLPKLARYGIVAGLAATWRATYYAPETMRQWLTRRDGADGSTATTTEATIR
                     RRLLTDSYLPYALANFVLLPAAFLPLGPWAATSALINSVFADVLTNLHTFLVVGPNHT
                     GDDLYRFASKPGSRGEHFARQVIGSTNYACGTNLVDFAHLWLNYQIEHHLFPDMPMLK
                     YQQMQPRVKALCDKHGITYTQEGVFRRFRKLVDVIVGDAQMGRA"
     gene            8785..9138
                     /locus_tag="VGB85_22995"
     CDS             8785..9138
                     /locus_tag="VGB85_22995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136976.1"
                     /translation="MVSDAAAAVRPARDPSLARAARIALAPIVFLATLCLLVALTGHG
                     PAISRLELFYTTMLSLAPVPVVLFGRTYRRLAAAARVPLGLSPRDAARPSLMSAIGDA
                     IRQTIVLAVGLAPSC"
     gene            9300..9917
                     /locus_tag="VGB85_23000"
     CDS             9300..9917
                     /locus_tag="VGB85_23000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136977.1"
                     /translation="MPKFLDGDGKTALTRAVAAFESGTAAELVVSVRPRSGTYPRAGI
                     LVGTLAALITTAFLLYSEPEFDLWWFLVWPAAVGLVLGHAADVRTLQWLLTSAATREK
                     RVLEAARAVFVERGIADTRGRTGILLYVSVAERIAVVIPDLGVRQAVPATDWDPAVAA
                     ITGAVARGATALELVAPIAALAAVARKHLPRATDDINELADAVHT"
BASE COUNT         1525 a         3908 c         3089 g         1496 t
ORIGIN      
        1 gaggcagaag cgtcgaggca agcccgcccg cgatctgtgc gagatcaaga ggagccttgt
       61 cgcttttggc tccgtgctat cctggccccc ggtgaagcgt cttcgtccct gcgggctcgc
      121 aaccccggtc tatctgttca gtttgctcgg agcctgcggc gacgatcaaa ccacgaccac
      181 cagcgcgagc acgaccaccg gaaccacgaa cgagacgagc acgaccaccg gcacaccgac
      241 ctccggtgct tctacatcct cttctacttc atccacctca tcgtcatctt catcctcttc
      301 gtcctccccg ggcgacccca ccgacgagcc gcctctcgag atcgcgcacg gcgtccggct
      361 gacgcgcgtg accgcgacgc agggcgtgca gaccgagctg gtccgcgacg gcgtcgaggt
      421 gcccgcggag gagtacgcgg tgccgctcat cagccgccgc aagacggtcc tgcgcgccga
      481 ctggctgctg catgccggct tcactgcgcg cccgctgatc ggccgcctga cggtgtggac
      541 ccccgagggg gacgtccacg tcgacacctc cgaggtcacc gtcgacggcc cgagcaacga
      601 cggcgacatg ctcaagacct ttgcctggca gctgccgccg gcgctggtcc gtccgggcat
      661 ggagtaccgc atcgaggcct tcgaggccga ccccacgctc gccaccggcg acatctccga
      721 tcctcctccg atcttgccgc tcgccggccg cggcgccctg atcgtcgagg accacccgat
      781 gcagatcagg gtcgagctga tcccgatcaa gcacgtgttc gcgggccaga cctgcatgcc
      841 ggagatcacc gacgccgacg tcaccgcgat gcggcaatgg atggagcagc acaaccccgt
      901 cgagcgggcc gagctcttcg tcggcgaacc actcgaatat accgcctcga tcggcaccgc
      961 ccaggacggt tttgtcccgc tgctgaccga gctcggcaag cgccgcgcgc tcgtcaagcc
     1021 gccccccgat ctctattact acggcctgat caccagctgc gacgcgtacc cgccgggcct
     1081 ggtcggccag gcctacggca tccccgacgc gccgacgatg aacttcgcct tccagcggat
     1141 cgccaccgga cgttacctcg gcagcggcgc cgcggcgcgc gacaccttcg tccacgagat
     1201 cggccacacc cagggccgct atcacgtccg ctgcagcggc ggcgagggcg gatgggaccc
     1261 cgactatccc tatcccaacg gccggatcgg cgtgtggggc tacggcattc acgacaccct
     1321 gctgcgctcg ccgaccggct ttcgcgacta catgagttat tgcaacacca gcttcgtctc
     1381 cgactacgga tggaagcaca cctataatta tattaaggag ctgtcgagct gggatgccgc
     1441 gtcggtcgca tccgacgagc cgatcttgct cgccacgatc ggcgagaacg gcgatatgag
     1501 cgggagcggc cacgcgacct ggtggaccac ccgcggccag gtcccgcagc gcgggcgcag
     1561 ccccggcacg cgggtcgagt tcaccgccgg tgggcaagtc tacacattgt ccgcgagcat
     1621 catcgagatc cccgattcac acgcgcacgc ggtcgccgtc gccttgcccg cgcgctggac
     1681 cgaggtcagc gagctgcgcc tgcggtccgc cggcaggccc gacctcgtca ccacccgcgc
     1741 cgcggtgacc gagctgcacg cgcgcccctg atcaaaccgc agcgtcagcc ggcgcgcacg
     1801 cgtgaccgag cgtatcaccc gcgtcggcgc gtcggcgcgt cgccgcatcg gcgcgtcggc
     1861 gcgtcgccgc gtcggcgcgt cggcgcgtca ggggtcgtcc ggctcggccg gctgcaagaa
     1921 cgtgtacggc gtcggcttcc agctggtcgg ccacgtgtgg tcgacgctcg ggcgattacg
     1981 catgcgctga tacagcgcgt gcaggtgcgg gtagtcctcg ccgagcccga gcccgaggtg
     2041 caccgccgtc gccaggtgca cgtacaccgc gaggtcggcg aaggtcagct ccgagccggc
     2101 cgcccacgcc gacacgccat aatagtactc ccagaagaac agctcgtcgt gcaacgcgtc
     2161 cgccagcgca tacacgcggc tcggctcgag gtccttgccc ttggtcttcg agagatagtc
     2221 gtagatctgc ccgcagacct cggagaggtt gtgggcctcg tgcaggcggg tcaacgccag
     2281 cgcgcgcagg gcgttggtcc ccggcagcag cggcgggtcc gggtgcgcgt actcgaggta
     2341 ctcgatgatc gcaaacggct cggacaccac gatcgatccg tcggtcagca ccgggaaagt
     2401 gccgcgcggg ttacgggtca gcagctcgcg gctcgcgtcc ttgccggtgg tcggcggcag
     2461 gagcttgacc tggtacggca ggtccttctc ctccagcgcg agctggatca tccacgacgc
     2521 cggcgagctc tcgaccacgc ggaccgtgct gcactccggg aggtacagct tgatctcgga
     2581 cgacgtcatg caacctcggg gttaacagat cgcgccagat cgcgtcaagc gcgacccccg
     2641 ggccggacgc ccccggggac gcgcggggac gggcgcgaac ggccccgtca atggcgcgtc
     2701 tgcccgggcg aagtatactg cgcccgccat gagcgagccc acgacctccc agaccccacg
     2761 tcgcgtcgtc atcgtcggtg gagggtttgg cgggctgtgc gcgggcatgg gcctcgccgg
     2821 cgccaggggt gtcgaggtca cggtcatcga ccgccgcaac caccacctgt ttcagccgct
     2881 gctgtatcag gtcgccatgg ccggcctgag cccggccgag atcgcgtacc cgatccgcac
     2941 gatcttcagc ggcaagcgca acatccgggt cctgctcgcg cgggccaccg gcgtcgacct
     3001 acagcgcaag atgttgcgca ccgacgacac cgagatcccg tacgacaacc tgatcctcgc
     3061 ctgcggcgcg cggcacagct acttcggcca caacgagtgg gagacctacg cgcccggcct
     3121 caagtcgctc gaggaggcga ccgagatccg ccgccgggtg ttgaccgcct tcgagctcgc
     3181 cgagaaggag cccgaccccg acaagcagcg caagctgctg accttcgtcg tcgtcggcgg
     3241 cgggccgacc ggcgtcgagc tggccggcgc cctcggcgag atcagccgct tcacgctctc
     3301 gaaggacttt cgccggatcg acccccgccg cacccgcgtg atcctcatcg aggccgcccc
     3361 gcggatcctc gccgcgttcg acgagaccct cgccaaccgg gccaccgccg acctcgagtc
     3421 gctcggcgtc accgtctgga cctcgaccaa ggtcacccgc atcgacgagg acggcgtcga
     3481 cctcggcgag gagaccgtcc aggccgccac gctcctgtgg gccgccggcg tcgccccctc
     3541 cgagctcaac aagagcctcg gcgtccccct cgaccgtggc ggccgcgtca tcgtcgaaaa
     3601 agactgctcc ctcaaggacc accccgaggt gttcgtcctc ggcgaccagg ccaacttcac
     3661 cggcgaggac ggcaaacccc tccccggcct cgcccccgtc gccatgcagc agggccgcta
     3721 cgtcgcccgc ctgctcctcg gcgagctccg cggcaagccc cgtgtgccct tcaagtacaa
     3781 cgacaagggc atcatggcga cgatcggccg ccgccgcgcc atcgtgcaat acggccgcct
     3841 caagttcgcc ggcttcttcg cctggctcgc ctggctgttc atccacgtcc tctacctcat
     3901 cggcttcaaa aaccgcatcg aggtcatgct caactggggc tggaactact tcgccttctc
     3961 ccgcggcgcc cgcctcatcg tcggcaagga ctggcgcgcc gccgccaact cccccgtccc
     4021 caacgccgtc gtcgacatgc cctcggcccc ccacccgatg gccggccccc agacccccgc
     4081 cgccccaccc gcgcccgccg cctgagcgtt tgcgctcgca aagccagcct cgacggcccg
     4141 cgtgagatct tcacgcgggc cgttggtgtt agacgcgact cggtgagccg ctcgagcagc
     4201 attgcagcat tgcagcattg cagcattggg tgccgccgcc ggagcgaccc atctgtgcga
     4261 cttcctgctc gcgcgctgac ctgcgaacgc gcaagtactt acggcacagc ccgctattta
     4321 acctctcgcg tgcgctagcc agaaaccgtc cgcgcgcaaa cagaccggca ccggccgacc
     4381 cgttcggggc ctttcacgcc cgtccaggta aaaattgccg gacctgggat gatccctgtg
     4441 agggggccgt tcgtataatg cggggccggc gccagcgcgc cgccctggag tttggatgtc
     4501 atccctcgaa tccagcagcc ccctgccgat ggacgccgcc ccgcacagcg aggcagacgc
     4561 cgcgcgcctc gacatgagcg gctcgttgta catgcggctg gtcgacggcg gcagcatgac
     4621 tgtcttcttc ctgctcgagt acttgctggc gatcgaggtc taccagggtt tttataaact
     4681 cgacgccacg gcgcacgccg gctggctggt gccggtcgcc ttcatggccg gctacatcac
     4741 cgccgacttc gcctcgggct ttttccactt cctcgccgac aactacggct cgacgaccag
     4801 gcccttcatc ggtccggtgt tcatccgtcc gttccgcgag caccacatcg acccccgggc
     4861 gatcacgcgg cacgacttcc tcgaagtcaa cggcgccaac tgtgcgatga gcgtgccggt
     4921 cctgctggcg acctggatgt tcctgccggt cggggccaac ctcgtcaccc tgttcctcgg
     4981 cgcctacgtc ggcctgttcc tgttcggcat cttcctgacc aaccagttcc acagctgggc
     5041 ccacgtcgag cggccgccgc ggatcgtccg tgccttgcag cgctcgggcc tcatcctcgg
     5101 ccccgagcac caccagcgcc accacaccgc cccgttcaac acctactatt gcatcaccag
     5161 cggctggctc aacccgctgc tcgcccgcag ccgcttcttc gagcggatcc atgaaccact
     5221 gcgtcgcgtg ctcgagcccc tgttcggcgt cgccgacgag gtcggcgcca cggcaccgac
     5281 cgaataacta caacccgagg ctgcggttgg tcgccgcgtc cgtccgctgc gcgctctcga
     5341 cgatcgactc gtacaccgcg tcgcccgcga gacccttctg ctgcgcgcgc tcgacgaggt
     5401 attcgaagct cggcccgtcg ggcgtgccgt acttctcctg gtcgcgcgcg cgcagggcct
     5461 cgaccgcggc gctgtcctgc atcatcgccc gcgcggtcac gcgcgcgtcg tgccgcgcct
     5521 gaaaggccgc ccgcgcccgc gcctcggccg tctgcccctg tgcccgcagc gccgcgtcgt
     5581 gcggcccgat ctccgcgacg cgctcgaggt acagctggcg gacctgacgg tcggtccacg
     5641 cgaccccggc ggcgcgcagc gaggccgcct cggccgcgtc ctttggcttc gccagtccgg
     5701 acggccatgg gtctccgggc gcgaccgcgg gctccgtcgc ctcgcaccca ccgaccgcgc
     5761 cgggcggcac cgtcacgccg agacacagcg tcaaccacca cgcgagcgga cccctgcgca
     5821 tcgccggcag tatcgccagg gcccggaccc gccgcaagcc gtgtaagctc cccgccgtca
     5881 gccgcgttcg ggctgcatca catcaccgcg ctcccgcgcg gctcctcgcc gcgcccacgc
     5941 cggccctctc gcaccgaggc tccccgatgt cgcgattgcc actactcttg tgttgcgccc
     6001 tgctcggcct cgtcccgggc tgcgccaaac gagcggacta cgagtcctcc gcgccgtccc
     6061 ccgagagcgc cagcatgggc ggcggcgggg acggagcgac cctgaccgac gtggccgctc
     6121 cggggccgat gcccgcctcc tacgacggcg acttcgccac gaagtccacg ggcgactatg
     6181 aggcgcgcga cgagatggcg ccgacctccg tgcaggccgc cgcggcccgc ggtaccccga
     6241 cgggtggcgt cgccgcggac accggcaagc tcgcggagcc gaggccggtc actccggagt
     6301 cgcgcgaggg caagggcacc gccgcccagg aggacgacaa aaagacctac gcgcgccaga
     6361 tcatctacac cgcggagatg cagatctcgg tgtacaagct cgacgacgcg atgcaacgcg
     6421 ccgaggagct gaccctcgcc gccggcggtt acattcagaa cctctcgcag ggctactacg
     6481 tcctgcggat ccccgcgcct gcgctgcgcg gcgtgatgga cgacctcgcc aggctcggcg
     6541 tcgtcgaggc gcgcaccctc caggccagcg acgtcaccga gcagtacgtc gacctgacca
     6601 cccgcatcaa ggtcctgcgc gagacccagg cccagctgct gagcctcctc aagcaggccc
     6661 gcaacgtcga ggaggcgctc aaggtccgcg cctcggtcga cgccgtcacc atggagctcg
     6721 agcaggcgat gggccgcctg cgcatgctcg agagcctgat cggctttagc accctgaccg
     6781 tgcgcatgag ccagcgcggc ccgcagaaca ccgtgccctc gagcaacgac cccttcccct
     6841 gggtcgacgg cctgggcgtc gaggcgacgg agtggaactg acatgacata cttcaagaca
     6901 tgttccctgg gcctggtcgc cgccgccgtg ctgtcgggct gcgccgccta caagatcgac
     6961 ccgccggtcg gcttcgccgc cgtcagcgag cacgactacg agacccgcat gaaggcgcag
     7021 gacaacgtcg gcctcagcgt gcgccgcttc gagaacgtca agggcggcac gctcgcgttc
     7081 tggggcgccg acctcgtcaa gaagctcggt gaccgcggct acatgctcag gaaccagagc
     7141 gcgatcaaga ccaaaaacgg caagaccggc acccgcttcg acttcgacta cgagaccttc
     7201 gacaccgaga tcccgaagtt ctacaccgcg atcctcgtcg tcaccgacaa gtacaaggtc
     7261 gtgctgcaga tcgccggcgg caaggagttc gcctccacct acgaggcgcg cgtcccggag
     7321 atcgtcggcc agctcaaggt ccgcggttgt aagctcggct cgaagatctg cggcggcccc
     7381 cagccaccca ggctctcgac ccccaagcca cccgccaaga aggtcgccac cgacgaaccg
     7441 gccgagccgg ccgcctcccc caccgccccg aaaccctgag cgcacacgcg caccgatcga
     7501 cccgcgcaga cacttgcgcg ggtgtcacga taacttcgat aaacatcgtt ggcgcgacgc
     7561 gtggtatggc ggccggcccc catgcacgac tccgcagcgc cgagcgagcc cgccccgtcc
     7621 ccgccgcctc gcgcgcccgg ccccctcgac cacgtcgact tcgccgcctt cctcgccgac
     7681 atccaggccc tgcgccgcga gctcgacgac agcctcgagg aggccgactt caagcatttg
     7741 caaaaaatgg agcgctgggg tcgggccgcg accgccgtcg gcctgctgac ctgctggatc
     7801 ggccccaacc cgctgagcgc cgcggcgctc ggcctcggcc gctcgacgcg atggttgttg
     7861 atgcatcacg tcggtcaccg cggctacgac aaggtgcccg gcgtgccgcc gcgctacacc
     7921 agcaaggtgt tcgcccgcgg cgcgcggcgc tggctcgact ggctcgactg gatcgtgccc
     7981 gaggcgtgga tctacgagca caacgtcctg caccactcgc acactggcga ggagcgcgac
     8041 cccgatctcg tcgagcgcaa caccgaggcc ctgcgcgcca agaatttacc gaagctcgcc
     8101 cgctacggga tcgtcgccgg cctcgccgcg acctggcgcg ccacctacta cgcgcccgag
     8161 accatgcgcc agtggctgac gcgccgcgac ggcgccgacg gctccaccgc gaccaccacc
     8221 gaggcgacca ttcgcaggcg cctgctcacc gacagctacc tgccctacgc gctcgccaac
     8281 ttcgtgctgc tgccggccgc gttcttgccg ctggggccgt gggccgccac cagcgcgctg
     8341 atcaactcgg tgttcgccga cgtgctcacc aacctccaca ccttcctggt cgtcggcccc
     8401 aaccacaccg gcgacgacct gtatcgcttc gccagcaagc ccggctcgcg cggcgagcac
     8461 ttcgcccgcc aggtcatcgg ctcgaccaac tacgcctgcg gcaccaacct cgtcgacttc
     8521 gcccacctct ggctcaatta ccagatcgag caccacctgt tccccgacat gccgatgctc
     8581 aagtatcagc agatgcagcc gcgcgtgaag gcgctgtgcg acaagcatgg catcacctat
     8641 acgcaggagg gcgtgtttcg ccggttccgc aagctggtcg acgtcatcgt cggtgacgcg
     8701 cagatgggcc gcgcataacc tccggcgcga ccatggacgt cgaagccgtc gcctcccctc
     8761 ccccccgcgc ggcctcccgg ccctgtggtt agcgatgcag cagccgctgt acggcctgcg
     8821 cgtgatcctt cgctcgcccg cgctgcccgc atcgccctcg cgccgatcgt cttcctcgcc
     8881 acgctgtgcc tgctggtcgc gctcaccggc cacggcccgg cgatctcgcg gctcgagctg
     8941 ttctacacga ccatgctgag cctcgcgccg gtccccgtgg tgctgttcgg acgcacctat
     9001 cgccgcctcg ccgcggccgc ccgcgtcccg ctcggcctgt cgccgcgcga cgcggcccgc
     9061 ccctccctga tgtcggcgat cggcgacgcg atccgccaga cgatcgtgct cgccgtcggc
     9121 ctcgccccaa gctgctgatc ccgatgggcg cgctcgtgtt tcgccccgcg accgtggtcg
     9181 gcggcgtgca tctgctcggt cggctcgaag atcgcggacc agccccgcgc gatcccgcga
     9241 tgtgatcgtg acaccgccgc ggcttggcag gctcgccgcc gtggccgaca atctctcgca
     9301 tgccgaaatt cctcgatggc gacggcaaga ccgcgctgac gcgggcggtc gccgccttcg
     9361 agtccggcac cgccgccgag ctcgtggtct ccgtccgccc gcgctcgggc acctaccccc
     9421 gcgccggtat cctcgtcggc accctcgccg ccctgatcac caccgcgttc ctgctgtaca
     9481 gcgagcccga gttcgacctg tggtggttcc tggtctggcc cgccgcggtc ggcctcgtgc
     9541 tcggccacgc ggccgacgtt cgcaccctcc agtggctgct caccagcgcc gcgacccgcg
     9601 agaagcgcgt gctcgaggcc gcccgcgccg tgttcgtcga gcgaggcatc gccgacaccc
     9661 gcggccgcac cggcatactc ctctacgtca gcgtcgccga gcgcatcgcc gtcgtcatcc
     9721 ccgacctcgg cgtacgccag gccgtgcccg ccaccgactg ggaccccgcc gtcgccgcga
     9781 tcaccggcgc cgtcgcccgc ggcgcgacgg ccctcgagct cgtcgcgccg atcgccgccc
     9841 tcgccgcggt cgcccgcaag cacctgccac gcgccaccga tgacatcaac gagctcgcgg
     9901 acgcggtgca cacatgagac ctgccccaaa gaccatgtcc cgtgccatcg tcgtcaccgc
     9961 cgtgctcggc ctcggcaccc tcgccctgct cgtcgcgcca cacctcgccg cggcccgc
//
LOCUS       DASWNJ010001382         2469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_308198, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001382 DASWNJ010000000
VERSION     DASWNJ010001382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308198"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VGB85_23005"
     CDS             <1..441
                     /locus_tag="VGB85_23005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0136978.1"
                     /translation="GAIGTENKKTLTLLIVDDERGILNTLEESFRDMFRVHTANSGAE
                     ALQIFRDQEIHLVLSDQRMPEMTGVELFGKLKAINPTPVRILITGYADINVVIRGLNE
                     GLMWQYVTKPWDTEELKTLLRRAAQHYLKETQNDEPDPIRGLSF"
     gene            772..1155
                     /locus_tag="VGB85_23010"
     CDS             772..1155
                     /locus_tag="VGB85_23010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136979.1"
                     /translation="MNLKTVNALLVEVEDGLMGGCAATDVFSKSSGMSVAGIRSSPKA
                     CALFNILCERIGETIKKSGLPVPDYLHQTMLRLGEDGSVMIVVIDLDVKYRWGMAVDC
                     TKAQLGILVSVVIPDTLPRLREALR"
     gene            complement(1267..2025)
                     /locus_tag="VGB85_23015"
     CDS             complement(1267..2025)
                     /locus_tag="VGB85_23015"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEY0136980.1"
                     /translation="MADLLSLAPGEVHVWLTTPETIVEPGLLATYAAWMNPEETARHA
                     RFLFARHRQQFLVARALVRTTLSRYVDLEPTAWRFTNNQWGRPDIDPAHGLGDLRFNL
                     SHTDGLVAVAVARGELGVDVEDTWRRSHTDQIAEHFFAPAEVAALRALPPERQHGRFF
                     ELWTLKEAYIKARGMGLAIALHSFAYALDGPRIGITIDDTHGDVADGWQFMLGAPTER
                     HRLALAVRFPGGEVAQVRVIEVVPGAAGSVLVRP"
     gene            2120..>2469
                     /locus_tag="VGB85_23020"
     CDS             2120..>2469
                     /locus_tag="VGB85_23020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY0136981.1"
                     /translation="MPSPIGVLLVNLGTPDAPQAPEVRRYLREFLSDPRVLDISPIGR
                     WMLLNLVILPTRPAKSAEAYRKVWRPEGSPLLHFSRALTDEVRAALPDMQVELAMRYG
                     NPSIRAGLTALRDRG"
BASE COUNT          382 a          903 c          794 g          390 t
ORIGIN      
        1 ggcgccatcg ggaccgagaa caagaagacg ctgaccctgc tcatcgtcga cgacgagcgc
       61 ggcatcctca acaccctcga ggagagcttt cgcgacatgt tccgggtgca caccgcgaac
      121 tccggcgccg aggcgttgca gatcttccgc gaccaggaaa tccacctggt gctcagcgac
      181 cagcgcatgc ccgagatgac cggcgtcgag ctgttcggca agctcaaggc gatcaacccc
      241 accccggtcc gcatcctgat cacaggttat gcggatatca acgtggttat ccgcggcctc
      301 aacgagggtc tcatgtggca atacgtcacc aagccctggg acaccgagga gctcaagacc
      361 ctcctgcgcc gcgccgccca gcactacctc aaggagaccc agaacgacga gcccgacccg
      421 atccgcgggt tgagcttcta gctcgggttc cggttctggc ccggggttcg ctcgaggggt
      481 tccactcggg ccttcgccct cgactccagg tcgcgactcc agctggtttg cgacacgtgc
      541 tcagtgcgag cagcagtgtc gaggccagac ccctgtgacg gcccgccctg ccccctcccg
      601 gcccgatctg ggccgtggcg gggtgagaaa atgttggagt cgtcaccaga agcgagctag
      661 gatgtcgggc gttcggcgct cgccggcccg ggactcgcgc ccgcggcccc gccgactcgc
      721 caaaattcgc cgatcacgcc gatcacgtcg cacggacacg aggaactcga aatgaatctg
      781 aagaccgtaa atgcactgtt ggtggaggtc gaggacggcc tgatgggtgg ctgcgccgca
      841 acggacgtgt ttagcaagtc gtccggcatg tccgtcgccg gtatccgcag ctcccccaag
      901 gcctgcgccc tgttcaacat cctgtgcgag cgcatcggcg agacgatcaa gaagtccggc
      961 ctgcccgtgc ccgactatct ccaccagacc atgctgcgcc tcggcgagga cggcagcgtc
     1021 atgatcgtcg tcatcgacct cgacgtgaag taccgctggg gtatggccgt cgactgcacc
     1081 aaggcccagc tcggcatcct ggtcagcgtc gtcatccccg acaccctgcc ccgcctgcgc
     1141 gaagccctcc gctgagcgcg acgccccttc tgtctgcacc cgtcaggcgt ccgtccgcac
     1201 cgcggccggg cgccgtcgtg ctttcggctt ttgctttcgg ctttttgctt tcgggcccgc
     1261 cggctttcac ggccgtacga gcacgctgcc agccgccccg ggcacgacct cgatgacccg
     1321 cacctgcgcg acctcgccgc ccgggaagcg caccgcgagc gcgagccggt gccgctcggt
     1381 cggcgcgccg agcatgaact gccagccgtc ggcgacgtcg ccgtgcgtgt cgtcgatcgt
     1441 gatcccgatc cgcggaccgt cgagcgcgta cgcgaagctg tgcagcgcga tcgccaggcc
     1501 catgcctctt gccttgatgt acgcctcctt gagcgtccac agctcgaaga agcggccgtg
     1561 ctggcgctcc ggcggcaggg cccgcagggc ggccacctcg gccggggcaa agaagtgctc
     1621 cgcgatctgg tcggtgtgtg agcgccgcca ggtgtcctcg acgtcgacgc cgagctcgcc
     1681 gcgcgcgacg gccacggcga ccagcccatc ggtgtgcgag aggttgaagc gcaggtcgcc
     1741 gaggccgtgc gcggggtcga tgtccggccg accccattgg ttgttggtga agcgccacgc
     1801 ggtcggctcg aggtcgacgt aacgcgacag cgtggtccgc acgagcgccc gcgcgaccag
     1861 aaattgttgg cggtgccgcg cgaacaggaa ccgggcgtgc cgcgccgtct cctccgggtt
     1921 catccacgcc gcgtaggtcg ccagcagccc cggctcgacg atcgtctccg gcgtcgtcaa
     1981 ccacacatgg acctcgccgg gggcgagcga gagcaagtcg gccatttgtc ggcaacgctt
     2041 gcaggctctc gcggtcgcgg tcaagaggcc gcgggttgca ggcgcgggcg ccccggtcca
     2101 ccatggcgac ccgctgccca tgccaagccc gatcggcgtc ctcctcgtca acctcggcac
     2161 tcccgacgcc ccgcaggccc cggaggtccg tcgctacctg cgcgaattcc tgtccgaccc
     2221 gcgcgtgctc gacatctcgc cgatcggccg gtggatgctc ctcaacctcg tcattttgcc
     2281 gacccgcccg gcgaagtccg ccgaggcgta ccgcaaggtc tggcgccccg agggctcgcc
     2341 gctgctgcac ttcagtcgcg ccctcaccga cgaggtgcgc gccgccctgc ccgacatgca
     2401 ggtcgagctg gcgatgcgct acggcaaccc gtcgatccgc gccggcctga ccgccctgcg
     2461 cgaccgcgg
//
LOCUS       DASWNJ010001383         2901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_556156, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001383 DASWNJ010000000
VERSION     DASWNJ010001383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556156"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1609)
                     /locus_tag="VGB85_23025"
     CDS             complement(80..1609)
                     /locus_tag="VGB85_23025"
                     /inference="COORDINATES: protein motif:HMM:NF040941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0136982.1"
                     /translation="MTTRSAPRSRPALLLLLLLPACAGGVESTTFGAQTTPSTAPGPT
                     GTGTGDTSDTGTPTGGGPDMGVATTGALPVCGNGTVEGDEQCDDGNADDADSCTADCK
                     AAACGDGIVQPPEVCDDGNTDDADACSNLCAAAACGDGVIQPPEACDDGNSEDADTCT
                     STCQAAACGDGFTQPGEACDDGNQTNDDDCTNACALASCGDGAPQPGEQCDDGNKDDT
                     DACLGTCLTASCGDGVVQAGVEACDDGNQSNLDACTVACALPACDDQIKSGSESDVDC
                     GGAACNDCNKGKSCLADTDCITGACVNGGCNLPTSCKQLKNGLPATTTGVYTLDTDGD
                     GPKQPFDVYCEMATDGGGWTLAGRSRNTPSAPGCAANDGLDGFGWRTATGSLADDSQA
                     YSLDVAGRGLAFTQVLFGNHAGGKTLAGFAYRHTVQANFIDVHINSHYAIGVPTTVTG
                     PCGAPFMFNYIGFTGNTNSFHFRDVDANDFGLFISGWRSCYDDCGGGNLNGQPGLLFV
                     R"
     gene            complement(1782..2336)
                     /locus_tag="VGB85_23030"
     CDS             complement(1782..2336)
                     /locus_tag="VGB85_23030"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEY0136983.1"
                     /translation="MTDKPTDQEDAYFRKQDQELRQRLRDQLSTSAGELDGRQGSELS
                     IPQRLEALGFAGSKLQVFDLMPLIHVAWADGSVSRQEKTAILDVVRERGIAPGSDAYL
                     LVETLLEARPSEQFLTQSLAILKEVIGDARAENIVELCLQIANASGGFMNIGNVSADE
                     RRQIEAIAGKLGPAALDVFRRELG"
     gene            complement(2383..>2901)
                     /locus_tag="VGB85_23035"
     CDS             complement(2383..>2901)
                     /locus_tag="VGB85_23035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136984.1"
                     /translation="PAIDAPVAAMADRFAPRRRLPLLAPLGPMPVEAQEASVLLEPGD
                     PAGATDPTPRAPRKPRAPLPSLPSSPPAAADASLAVDPRAPGAPPRLLFDKRAESIAP
                     GVYACMQQHSPELRTLRLALTVGGPRHTPAAMALPEHDTPELRRCIARLLLRLDFPAT
                     EGTARYEHVYRR"
BASE COUNT          348 a          969 c         1139 g          445 t
ORIGIN      
        1 tcagccgtga gacccgccgc atgatctcat catcacatgc gagcgccgcg ctgtcgacat
       61 gcagccgggc cgagccggct cagcggacga acagcaggcc gggctggccg ttgaggttgc
      121 cgccgccgca gtcgtcgtag cacgagcgcc agccgctgat gaacaggccg aagtcgttgg
      181 catcgacgtc gcggaagtga aagctgttgg tgttgccggt gaagccgatg tagttgaaca
      241 tgaacggcgc gccgcacggg ccggtgaccg tcgtcggcac gccgatcgca tagtgactgt
      301 tgatgtgcac gtcgatgaag ttggcctgca ccgtgtggcg gtaggcgaag ccggcgaggg
      361 tcttgccgcc cgcgtggttg ccgaacagca cctgcgtgaa cgcgaggccg cggccggcga
      421 cgtcgaggct gtaggcctgg ctgtcgtcgg cgaggctgcc ggtcgcggtg cgccagccga
      481 agccgtcgag gccgtcgttc gcggcgcagc cgggggcaga aggcgtgttg cgcgagcggc
      541 cggcgagcgt ccagccgccg ccgtcggtcg ccatctcgca gtagacgtcg aagggctgct
      601 tcgggccgtc cccgtcggtg tcgagcgtgt acacgcccgt ggtggtcgcc ggcaggccgt
      661 ttttgagctg cttgcacgag gtcggcaggt tgcagccgcc gttgacgcag gcgccggtga
      721 tgcagtcggt gtcggcgagg cagctcttgc ccttgttgca gtcgttgcag gccgcgccgc
      781 cgcagtcgac gtcgctctcg ctgccgctct tgatctggtc gtcgcaggcc ggcagggcgc
      841 aggcgacggt gcacgcgtcg aggttgctct ggttgccatc gtcgcaggcc tcgacgccgg
      901 cctggaccac gccgtcgccg cagctggccg tcaggcaggt gccgaggcag gcgtcggtgt
      961 cgtccttgtt gccgtcgtcg cactgctcgc ccggctgcgg cgcaccgtcg ccgcacgagg
     1021 cgagcgcgca ggcgttggtg cagtcgtcgt cgttggtctg gttgccgtcg tcgcaggcct
     1081 cgcccggctg ggtgaagccg tcgccgcacg cggccgcctg acaggtcgat gtgcaggtgt
     1141 cggcgtcctc gctgttgccg tcgtcgcagg cctcgggcgg ctggatcacg ccgtcgccgc
     1201 aggccgccgc ggcgcacagg ttcgagcacg cgtcggcgtc gtcggtgttg ccgtcgtcgc
     1261 agacctccgg cggctgcacg atgccgtcgc cgcaggccgc ggccttgcag tcggcggtgc
     1321 agctgtcggc gtcgtcggcg ttaccgtcgt cgcactgctc gtcgccctcg accgtgccgt
     1381 tgccgcagac cggcagggcg ccggtggtgg cgacgcccat gtccgggccg ccgccggtcg
     1441 gtgtcccggt gtcgctggtg tccccggtgc cggtgccggt ggggcccggg gcggtggacg
     1501 gcgtggtctg ggcgccgaac gtggtgctct cgaccccacc ggcgcaggcc gggagcagga
     1561 gcaggagcag gagcgcggga cgggagcggg gagcggagcg cgtggtcacg gcgagaggcg
     1621 gcgccgggtg acggacgccg cgacatcgca gcatggcaga gcgccctcgc cgtgggaacc
     1681 gcggaggcgc gggcgaggcc gcgcacgccc gcgtcctccc ccacgttctt ccccgtgttc
     1741 ggggacctgg cccttgggtc aggtcctcgc tggacgaccg atcagccgag ctcgcggcgg
     1801 aacacgtcga gcgcggcggg gccgagcttg ccggcgatcg cctcgatctg gcgccgctcg
     1861 tcggccgaga cgttgccgat gttcataaag ccgccgctgg cgttggcgat ctgcaggcac
     1921 agctcgacga tgttctccgc gcgggcgtcg ccgatcacct ccttgaggat ggcgaggctc
     1981 tgggtgagaa actgctccga ggggcgcgcc tcgagcaggg tctcgacgag caggtacgcg
     2041 tcgctgccgg gggcgatgcc gcgctcgcgc acgacgtcga ggatcgcggt cttctcctgg
     2101 cgcgacaccg aaccgtcggc ccaggcgacg tggatcagcg gcatgagatc gaagacctgc
     2161 agcttgctgc cggcgaagcc gagggcctcg aggcgctggg ggatcgagag ctcgctgccc
     2221 tggcgcccgt cgagctcgcc ggcggaggtg ctcagctggt cgcgcaggcg ctgccgcagc
     2281 tcctggtcct gcttgcggaa gtaggcgtct tcttgatcgg tcggcttgtc ggtcatcgcg
     2341 gcccgaacct atcaggcgtg cgcgccttgt caatccagcg cgctagcggc gatacacgtg
     2401 ctcgtagcgg gcggtgccct cggtcgccgg gaagtcgagg cgcagcagca ggcgcgcgat
     2461 gcagcggcgc agctccggcg tgtcgtgctc cggcagggcc atcgcggcgg gggtgtgccg
     2521 tgggccgccg acggtcagcg cgagccgcag ggtccgcagc tcggggctgt gctgctgcat
     2581 gcaagcgtag acgccggggg cgatcgactc ggcccgcttg tcgaacagga gccgcggcgg
     2641 agctccgggg gcgcgcgggt cgaccgcgag gctggcgtcg gccgcggccg gaggcgatga
     2701 cggcaacgac ggcaacggcg cccgcggctt gcggggcgcc ctcggggtcg ggtccgtcgc
     2761 gcctgcagga tcgcccggct cgagcagcac gctggcctcc tgcgcctcga ccggcatcgg
     2821 gccgagcgga gcgagcagcg ggagccggcg tcgcggcgcg aagcggtccg ccatcgcggc
     2881 gaccggcgcg tcgatcgccg g
//
LOCUS       DASWNJ010001384         2705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_483931, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001384 DASWNJ010000000
VERSION     DASWNJ010001384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483931"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VGB85_23040"
     CDS             complement(<1..306)
                     /locus_tag="VGB85_23040"
                     /inference="COORDINATES: protein motif:HMM:NF024818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0136985.1"
                     /translation="MNVDAWDGVPVPMIGWREDGRVVAVNTAAVSALGRSREDLLATS
                     YWELTLSGQKQRELALVQRCELPFGKEFLRASGEPVTVQVIGCTRLPDGDGHVCAFTV
                     "
     gene            complement(593..1270)
                     /locus_tag="VGB85_23045"
     CDS             complement(593..1270)
                     /locus_tag="VGB85_23045"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0136986.1"
                     /translation="MTTTPNDTGLQGSAERFERLMEVVSLASVGEYAAALAHCNPLQE
                     DSFGMLEEGLRVFIGELRDGQREREEAATALARAQEDIDAKLTLIEAQSREIRALSTP
                     ILDVWDDVLAVPLVGTLDHARTLEITEKLLQRVVERRARWSLIDLTGVDEVDEATADH
                     LVKLARAVALVGGRCVVTGVSPQAAQIFVSLGHGLGELRCLPNLKEGLRHCLAARRSP
                     LAGRAPG"
     gene            complement(1267..1686)
                     /locus_tag="VGB85_23050"
     CDS             complement(1267..1686)
                     /locus_tag="VGB85_23050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0136987.1"
                     /translation="MEQATIGRHRYQKAGDVVRLQWQGNISADDLKVMREVTTRMMQE
                     LGRAFTISDMSQVTGIDAGARKYMAEWGQQDGNGLITGTAVYGLSFATRAITTLALNA
                     ITLLGKKKVEVAFVKDEAEATRWVDEQRAARFPESAS"
     gene            complement(1739..2167)
                     /locus_tag="VGB85_23055"
     CDS             complement(1739..2167)
                     /locus_tag="VGB85_23055"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY0136988.1"
                     /translation="MSAAPYSGACLCNAIHFTVTGPPSTVCLCHCATCRRAVGSPGVA
                     WATFRRDALVLTGTPTWFRSSDHARRGFCGACGTSMFFETSHEPHAIDITVASLHAAE
                     RVAPRYHSWAPSKLPWQTITDGLPRFREDGGSLPMEVEPR"
     gene            2361..>2705
                     /locus_tag="VGB85_23060"
     CDS             2361..>2705
                     /locus_tag="VGB85_23060"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0136989.1"
                     /translation="MPVVLPHAFGRYTLTRRIGEGGMAEVFLAEVTVAEGLSKRVVIK
                     KIRKELADQPEFTRMFVDEAKIALSLNHANIVQVFDFGQVHGSFYLAMELIEGVDLMR
                     LLHTIRGRGERIP"
BASE COUNT          406 a          938 c          920 g          441 t
ORIGIN      
        1 gaccgtgaat gcacacacat ggccgtcgcc gtccgggagc cgggtacaac cgatcacctg
       61 caccgtgacc ggctcaccgc tggcgcgcag gaactccttg ccgaacggca gctcgcagcg
      121 ctggaccagc gcgagctcgc gttgcttctg tcccgagagc gtcagctccc agtacgaggt
      181 cgcgagcagg tcctcgcgcg atcgaccgag cgccgacacg gccgcggtgt tgaccgcgac
      241 cacccgacca tcctcgcgcc agccgatcat cggcactggc acgccgtccc atgcatcgac
      301 gttcactgtc cctgtcgtaa catcgttgct tcgtcacgaa agcgtctgca cgggcagtcc
      361 cgtatgctcg tggtcacgag cgacgggtcg tgaacgcgca tgtagacgca gttacggccg
      421 gtttacgtcg gtcttgcggc gaaacgctgg gcatctgtcc ggttcttgcg atcatgcttt
      481 cgtatttcgg cggccgttgc tggagcacat taaaatttgt gcccagagcg ggcacgtgcg
      541 tcgcgggccg gcgacgatcg acgatcgatg accgatgatc gatgaccgat gatcagccgg
      601 gggcgcgtcc cgccagcggg ctgcggcggg ccgcgaggca atggcgcagg ccctccttga
      661 ggttcggcag gcagcgcagc tcgccgaggc cgtggccgag cgacacgaag atctgggccg
      721 cctgggggct gacaccggtg acgacgcagc gcccgccgac cagggcgacc gcgcgcgcca
      781 gcttgacgag gtgatcggcg gtcgcctcgt cgacctcgtc gacgccggtc aggtcgatca
      841 ggctccaccg cgcgcggcgc tcgacgaccc gctggagcag cttctcggtg atctcgaggg
      901 tgcgcgcgtg atcgagggtt cctaccaatg gcaccgcgag cacgtcgtcc cagacgtcga
      961 ggatcggcgt cgacagggcc cggatctcgc ggctctgcgc ctcgatcaac gtcaacttgg
     1021 cgtcgatgtc ctcctgcgcg cgtgcgaggg cggtggccgc ctcttcgcgc tcgcgttgcc
     1081 cgtcgcgcag ctcgccgatg aagacgcgca ggccctcctc gagcatgccg aagctgtcct
     1141 cttgcagcgg gttgcagtgt gccagcgccg ccgcgtactc gccgacggag gcgagcgaga
     1201 cgacctccat cagccgctcg aagcgctcgg cggatccctg cagaccggtg tcgttcggtg
     1261 tcgtcgtcac gatgcactct ccgggaatct cgcggcccgc tgctcgtcga cccagcgcgt
     1321 cgcctcggcc tcgtccttga cgaacgcgac ttcgaccttc tttttgccga gcaacgtgat
     1381 cgcgttgagg gcgagcgtcg tgatcgcccg ggtcgcgaag ctgaggccgt acacggcggt
     1441 gcccgtgatc aggccgttgc cgtcctgctg gccccactcc gccatgtact tgcgagcgcc
     1501 ggcgtcgatg ccggtgacct gcgacatgtc cgagatcgtg aaagcccggc cgagctcctg
     1561 catcatcctg gtcgtcacct cgcgcatcac cttgaggtcg tccgccgaga tgttgccctg
     1621 ccactgcagg cggaccacgt cgccggcctt ctgataacga tgccgcccga tcgtcgcctg
     1681 ttccacgcgc ggcaggctac cacacgggtc cgacgcgggg ctccgcgcgg gttcgccact
     1741 agcgcggctc gacctccatc ggcagcgatc caccgtcctc gcgaaagcgc ggcaggccgt
     1801 ccgtgatcgt ctgccagggc agcttgctcg gcgcccacga gtggtagcgc ggggcgacgc
     1861 gctcggcggc gtgcagcgac gcgactgtga tgtcgatcgc atgcggctcg tggctggtct
     1921 cgaagaacat cgaggtgccg cacgcgccac aaaagccgcg gcgcgcgtgg tcggacgagc
     1981 gaaaccacgt cggcgtgccg gtgagcacca gcgcgtcgcg gcgaaacgtc gcccacgcga
     2041 ccccgggcga tccgacggcg cggcggcagg tcgcgcagtg gcacaggcac accgtcgacg
     2101 gcgggccggt gacggtgaag tggatcgcgt tgcacaggca agctccggag tacggcgcgg
     2161 cggacatgtc cgcgagtatg gcagtgacgc cgtcgcgcac gctacgcagg ccctgggcgt
     2221 gcgtgagttg ccgcgcgatc tccgccgcat gcaccgattg gctcgtgcgt accccgggga
     2281 tcagcgtcgc cgcgatgtga gcatcgtggt ccgggccgcc gcccacgggg gcggttcgtg
     2341 gttaagctcg cgtctccccc gtgcccgtcg tcctccccca cgcgttcggc cgctacaccc
     2401 tgaccaggcg catcggcgag ggcgggatgg cggaggtgtt cctcgccgag gtcaccgtcg
     2461 ccgagggcct gagcaagcgc gtcgtcatca agaagatccg caaggagctc gccgatcagc
     2521 ccgagttcac gcgcatgttc gtcgacgagg cgaagatcgc gttgagcctc aaccacgcca
     2581 acatcgtcca ggtcttcgac ttcgggcagg tgcacggcag cttctacctg gcgatggagc
     2641 tgatcgaggg cgtcgacctg atgcgcctgc tgcacacgat ccgcggccgc ggcgagcgga
     2701 tcccc
//
LOCUS       DASWNJ010001385        10859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_483936, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001385 DASWNJ010000000
VERSION     DASWNJ010001385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 10859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10859
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483936"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VGB85_23065"
     CDS             <1..407
                     /locus_tag="VGB85_23065"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY0136990.1"
                     /translation="RDAADAFLEVLDAEKPMPETVVVHCFTGDGATLRQYLRRGYNIG
                     ITGWICDERRGLHLRALVKEIARDRLLVETDAPFLTPRDLKPKPAAGRNEPAFLPHVL
                     RTIARCRGEDPEALAERTTANALRVFPRLTQG"
     gene            complement(409..2190)
                     /locus_tag="VGB85_23070"
     CDS             complement(409..2190)
                     /locus_tag="VGB85_23070"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEY0136991.1"
                     /translation="MPINQPPQSASGTPAGHPPHATVVGQPGFVPAGQGYPPAGSFVP
                     APGVQQPPSPVGPSFTPAAPPQQEPGYLSAYPPAAPAGAPTFLPEPVSPASPPPPAPP
                     PFRPSRNPIVRLILWVTEPSMIKGMAMGVILALVISVAFRLESMFFIASSVLTLLIVC
                     LFGVLIGHYIFENRRKKLQRRGIDLVAQAGGELPGLSNDLLTLLWNRDRAALTTVWER
                     LRRVRPAAQEIAGLSIAAVFRMMAMTTLFAVLGGAISFAVFLTSYMQVERMDAQNKLI
                     ATQIDQTNKQMELTEKQQSIEVALSIAERRQVTIREVLTLINNERPVLENGKRRLGGT
                     TANLIAVAVAQLEPYRPVAANVDGGTNELQEHVRSPEQEQLVRYLSAANVDFGELDLR
                     RAFLDHTDLQGTDLNGANLEKVRMRHVLLHDAKLAGANLALADLTLAGLARVDLSGTN
                     LHQAKLHRANLDDAVLTEANLAAADLSGAILKKAVFKQTQLGGARLHGADLTHCDLNG
                     ADLTDADLALADLSSATMPVVPKIRAAAFWWLAIYPPDYAAKLGLDAAALARNQAGLD
                     RIRKALDAPAIAAIVGELKAAHPSAPG"
     gene            2248..4095
                     /locus_tag="VGB85_23075"
     CDS             2248..4095
                     /locus_tag="VGB85_23075"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY0136992.1"
                     /translation="MNSDRETEARAWPAVPLEAWRELALRELGGAPLERLVSRTAEGL
                     RVEALHPGEPTELPGRALLLAHAGWTISVECSEPDAATAGLAIAEDLGRGAQAVWVRL
                     DEPGPVGLHGVVVRGADDLGKLLAGVDPTRTPVTLAVGADGSAVKDMLTQVLAARGLE
                     VAGLRGLLGCDPLGALARRGAIEGSIEQALSKMSEVAGWARTTAPGLRTALVDVGAYH
                     DAGATAADQLAALLATGVAYLKALMASGASVAEATRQMSFAVAVGRDLFLEIAKLRAA
                     RLCWARVVAACGGDASAQRMYLHARGSWRERTTIDPWVGLLRGTGETVAAAIGGADSI
                     ATTPMDAALGEPGALGRRLALNTQIILAEESHLGRVADPGGGAGQIESLTDQLARAAW
                     SRFQRIEAAGGMVAALRGGLVQADVAEAAKAQAQAVASVRVPIVGVSRFAAKDERPTG
                     ARVVERQIATEASGERVTPLVRARLAEPFEALRARSRRERVALVAVGEPAQWRARVEF
                     CRAYFEVGGFEVVETPGTQELAQAVQQFTETGARAAVICSAEAVYPTTVPELVPMLRE
                     AGAAAVLVAGRPKDQVEALTAAGVDLFVQLGGDAPALLGSLAQRLEVRS"
     gene            4092..6257
                     /gene="scpA"
                     /locus_tag="VGB85_23080"
     CDS             4092..6257
                     /gene="scpA"
                     /locus_tag="VGB85_23080"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HEY0136993.1"
                     /translation="MKNFPDFASLDYDATPTPAPAPFDERPVWRTPEGIDLHASYGAE
                     ALADVDPDFLRTQPGLPPYTRGPYATMYTQRPWTIRQYAGFSTAEESNAFYRRNLAGG
                     QKGLSIAFDLATHRGYDSDHPRVVGDVGMAGVAIDSILDMRILFNGIPLDQMSVSMTM
                     NGAVLPILALYIVAAEEQGVKPAQLQGTIQNDILKEFMVRNTYIYPPAPSMRIVADIF
                     AYTAEHMPKFNSISISGYHMQEAGATADLELAYTLADGAEYLRTGVAAGMAIDSFAPR
                     LSFFWGVGMNFFMEVAKLRAARLLWSRIVARFGAKNPKSLALRTHCQTSGWSLAAQDP
                     YNNVARTCVEALAATLGGTQSLHTNAFDEALALPTDFSARIARNTQIYLQEETNIGRV
                     IDPWGGSYHVERLTWDLAEKAWALIEEVEALGGMAKAIEQGLPKLRIEEAAARTQGRI
                     DSGAQTVVGVNRYRLAEEAPLDVLKVDNTAVRTAQLARLAQLRRERDSTEVRHTLAAL
                     TRCAGGESGNLLALAVEAARARATVGEISLALERVWGRHQATTRTVRGVYSSAVSDVG
                     SIEAVQALARAFSEAEGRRPRLLVAKMGQDGHDRGAKVIATAFADMGFDVDIGPLFQT
                     PAETALQAVENDVHVVGVSSLAAGHLTLVPQLQSELRRLGRADIMIVVGGVIPPHDHE
                     TLRTMGVAAVFGPGTVLTTAAEQIDRTLAQQLELDLGVG"
     gene            6291..8171
                     /locus_tag="VGB85_23085"
     CDS             6291..8171
                     /locus_tag="VGB85_23085"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY0136994.1"
                     /translation="MSLSVGIDLGTTRCAVGVMEAGKATTLLVNRQPYREPSVIGHEE
                     DGSSVIGERARRLALTRPREMMLAARRLLGRRFDEVDAEAWRAQPLLRAENGDVQVKL
                     GEHTVSTPELLAPLLRQVRQTTEAQLGETIPTVVVAVRGQLRDLQRRATLAACRLGGL
                     EPRRLIHTTTAAALVYHATRNSVGDERIAVIDFGGGGFDVALIEVGDRSVEVLAQRGV
                     EVGGEDLDARMIEWLLAELREQTGIDGAADPIVRARMRDAAEKARIALSEQKEVVVHL
                     PYLAADEAGPKHLASKLTQKRFEKLVADLLDRCAATVQRALDEAGRPIDQFTQVLFIG
                     GCTRMPALHERVATIFKKEPSAALDSDDVVARGAAIFAGLLASRSPELVVHEALSRPL
                     WLQVGRAEPRPLFPPRSPVPTEHSEPLETPGDGRPGVECRVLEGERAGGEAPLLLKFT
                     VAGRPEVETKFTLDGNQVLDIAIREMFRGKEARLVIEERGGLEGPVLAAMIEAARAEE
                     TEARAQRAIAERRQRLEGMAQRAERVTAEMEKLSPELRAAVTAGVRDARAVLQTQDDE
                     LVTAAIEAFAGLLRPLPGELAAIAAPPAPTGRLQPVPMAPPKRVVASDGGEFIPDDPD
                     QS"
     gene            complement(8173..8874)
                     /locus_tag="VGB85_23090"
     CDS             complement(8173..8874)
                     /locus_tag="VGB85_23090"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0136995.1"
                     /translation="MRGKCLPLILLLGLGACATRARTGPGPAMASGGGPMLADVTVDP
                     NADRDSDGFLDLQDTCPGEPGHEPDGCPARDSDRDQIFDVDDDCPDAPEAVNRFHDHD
                     GCPDEFPAAWAILDRPIFFGLGVDELRKRPMDELTPAIKAGLEQIAAVMREYPELRIE
                     IVGHHDSAWKLRGKQDPSARRVLAARKFLVEKQKIKTKRIHFRAEGSEQPIADNDAPD
                     GPLKNNRLELRILSN"
     gene            complement(8898..10373)
                     /locus_tag="VGB85_23095"
     CDS             complement(8898..10373)
                     /locus_tag="VGB85_23095"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0136996.1"
                     /translation="MRFGPIRLHYLLAYAIVGAYMPYLPVFLGRDLGMPDWQIGWIAG
                     MYGMAVILSPPIVTYLADRRLSGRTLIGLAYGLTGCTLLALAFARGFIPVLLISMVFS
                     MVYTPMFALLDGLTFSASAAAQAEGRPVPPYDRIRVWGSFGFMIPAFVLFFALRAGAA
                     SGRAAIFAAAVAAGLGLLCLPLLPRIAPGRPGPETSLPGAPVPETSLPGALVLDPGAP
                     VPETSLADAPVPATSLAGALIPGAPVQETSLAGALIPGAPVPETSLAGALVPGALVPG
                     APVPEASLTTPPSTTAWDVLRRPPTRDLIIPLGLLFASIAVFYGFYARLVLEVGIDPS
                     WVGLVMNLGVVSELPFMLAAGPLLRRYTLRQLIVFGALCLVVRMVLLASVADPRVIVA
                     SQLLHGPIVVAIYLIPPMYLNLKAPAGARNSVQGIYAMVCFGVARLLGSVAGGYAAGR
                     GLAWAFGLGAVLAATALVWLWLRFFDAAADTALRSRPLSRS"
     gene            complement(10380..>10859)
                     /gene="orn"
                     /locus_tag="VGB85_23100"
     CDS             complement(10380..>10859)
                     /gene="orn"
                     /locus_tag="VGB85_23100"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEY0136997.1"
                     /translation="ILEIATIITDGDLNLLAEGPDIVVHQPDPVLDAMDAWCTRQHAL
                     SGLTAQVKASTTTLIQAETQTFEFIRQHCPPGKSPLCGNSIGHDRRFLIKHMPVLASF
                     LHYRSIDVTSIKELARRWYPELPAPEKGETHRALDDIRESIAELRYYRQHIFRPRES"
BASE COUNT         1614 a         3464 c         4272 g         1509 t
ORIGIN      
        1 agcgcgacgc ggccgacgcg ttcctcgagg tgctcgacgc cgagaagccg atgccggaga
       61 cggtggtggt gcactgcttc accggggacg gggcgacgct gcgccagtac ctgcggcgcg
      121 gctacaacat cggcatcacc ggatggatct gcgacgagcg ccgcgggctg cacctgcgcg
      181 cgctggtcaa ggagatcgcg cgcgacaggt tgctcgtcga gaccgacgcg ccgttcctga
      241 cgccgcggga cctcaagccc aagccggccg cgggtcgcaa cgagccggct tttttaccgc
      301 acgtgctgcg gacgatcgcg cgctgtcgcg gggaggaccc cgaggcgctc gcggagcgga
      361 ccaccgcgaa cgcgctgcgg gtgttcccgc ggctgacgca aggctgactc agcccggtgc
      421 gctcgggtgg gcagccttga gctcgccgac gatcgccgcg atcgcggggg cgtcgagggc
      481 cttgcgaatg cggtcgaggc cggcctggtt gcgggccagc gcggcggcgt cgaggccgag
      541 cttggcggcg tagtcggggg ggtagatcgc cagccaccag aacgcggcgg cgcggatctt
      601 cgggaccacc ggcatcgtcg cgctcgagag gtcggcgagc gcgaggtcgg cgtcggtcag
      661 gtcggcgccg ttgagatcgc agtgggtgag gtcggcgccg tggagcctgg cgccgccgag
      721 ctgcgtctgc ttgaacaccg ccttcttgag gatcgcgccg gacaggtcgg cggcggcgag
      781 gttggcctcg gtcagcacgg cgtcgtcgag gttggcgcgg tggagcttgg cctggtggag
      841 gttggtgccg gacaggtcga cgcgcgcgag gccggcgagc gtcaggtcgg caagggcgag
      901 gttggccccc gcgagcttcg cgtcgtgtag cagcacgtgg cgcatgcgga ccttctcgag
      961 gttggcgccg ttgaggtcgg tgccttgcag gtcggtgtgg tcgaggaagg cgcggcgcag
     1021 gtcgagctcg ccgaagtcga cgttggccgc ggacaaatag cgcacgagct gctcctgttc
     1081 cgggctacgg acgtgctctt gcagctcgtt ggtgccgccg tcgacgttgg cggcgacggg
     1141 gcggtagggc tcgagctggg cgacggcgac ggcgatcagg ttggcggtgg tgcctccgag
     1201 cctgcgcttg ccgttctcga ggaccgggcg ctcgttgttg atgagggtga ggacctcgcg
     1261 gatcgtgacc tggcggcgct cggcgatgct gagggcgacc tcgatcgact gttgcttctc
     1321 ggtcagctcc atctgcttgt tggtctggtc gatctgcgtg gcgatgagct tgttctgcgc
     1381 gtccatccgc tcgacctgca tgtacgaggt caggaacacg gcgaagctga tcgcgccgcc
     1441 gaggaccgcg aacagggtcg tcatcgccat catgcggaac accgcggcga tcgacaggcc
     1501 ggcgatctcc tgggcggccg gccgcacgcg acgcaggcgc tcccacacgg tcgtcagcgc
     1561 ggcccggtcg cggttccaca ggagggtcag gaggtcgttg ctcaggcccg gcagctcgcc
     1621 gccggcctga gcaacgaggt cgatgcccct gcgctggagc ttcttgcgcc gattctcaaa
     1681 gatgtagtgc ccgatcagca cgccgaacag gcacacgatc agcagggtca gcacgctgct
     1741 ggcgatgaag aacatcgact cgaggcgaaa cgccaccgag atcacgagcg ccaggatgac
     1801 gcccattgcc atgcccttga tcatcgaggg ctcggtcacc cacaggatca ggcgcacgat
     1861 cgggttgcgt gacggacgaa acggcggcgg cgccggaggt ggtgggctgg ccggagagac
     1921 aggttcgggc aggaaggtcg gtgcgcccgc gggcgcggcc ggcgggtagg cggacaggta
     1981 gccgggctcc tgctgcggcg gagcggccgg cgtgaagctc ggacccaccg gcgagggcgg
     2041 ctgctgcact cccggcgcgg gcacgaagct gccagcgggg ggatacccct ggccggcagg
     2101 cacgaagccc ggctggccga cgaccgtcgc gtgcggagga tgtccggcgg gtgtgccgga
     2161 cgcggactgc ggtggttgat tgatgggcat ggccggggcg aggcgcacta tagctgcatc
     2221 gccgcggcgg gcgtatcatc gcggcgcgtg aacagcgacc gagagaccga ggcgagggcg
     2281 tggccagcgg tgccactgga ggcctggcgc gagctggccc tgcgtgagct gggcggagcg
     2341 ccgctggagc gcctggtgtc gcgcacggcg gaggggctgc gggtcgaggc gctgcacccc
     2401 ggcgagccga ccgagctgcc ggggcgcgcg ctgctgctcg cgcacgcggg ctggacgatc
     2461 tccgtcgagt gcagcgagcc cgacgcggcg acggccgggc tggcgatcgc agaggacctc
     2521 gggcgcggcg cgcaggcggt gtgggtccgg ctcgacgagc cggggccggt cggcctgcat
     2581 ggcgtcgtcg tgcggggcgc ggatgacctt ggaaaattgc tcgcgggggt cgacccgacg
     2641 cggacgccgg tgacgctggc ggtcggcgcc gatgggtcgg cggtcaagga catgttgacg
     2701 caggtgctcg cggcgcgcgg gctcgaggtc gcggggctgc gcgggctgct cggctgtgac
     2761 ccgctcgggg cgctggcgcg tcggggcgcg atcgaagggt cgatcgaaca ggccctttca
     2821 aaaatgtccg aagtcgcagg ttgggcgcgc acgacggcgc cggggctgcg cacggcgctc
     2881 gtcgacgtcg gggcgtatca cgacgccggc gcgaccgcgg cggaccagct cgcggcgctg
     2941 ctcgcgaccg gggtcgctta tttaaaggcg ttgatggcga gcggggcgag cgtggcggag
     3001 gcgacgcggc agatgagctt cgcggtcgcg gttggccgcg atctattttt ggaaattgcc
     3061 aagctccgcg cggcgcggct gtgctgggcg cgcgtcgtcg cggcctgtgg cggcgacgcg
     3121 tcggcgcagc gcatgtactt gcacgcgcgc ggctcgtggc gcgagcgcac gacgatcgac
     3181 ccgtgggtcg ggctgctgcg cggcacgggg gagaccgtcg cggccgcgat cggtggggcc
     3241 gactcgatcg cgaccacgcc gatggacgcc gcgctcggcg agccgggtgc gctcgggcgg
     3301 cgcctcgccc tcaacacgca gatcatcctc gccgaggagt cgcacctcgg tcgggtcgcc
     3361 gacccgggcg gcggcgcggg gcagatcgag tcgttgaccg accagctcgc gcgcgcggcg
     3421 tggagccggt tccagcgcat cgaggcggcc ggcgggatgg tggccgcgct gcggggcggg
     3481 ctcgtgcagg ccgatgtcgc ggaggccgcg aaggcgcagg cgcaggcggt cgccagcgtg
     3541 cgtgtgccga tcgtcggggt cagtcgcttc gccgcgaagg atgaacggcc gaccggcgcg
     3601 cgcgtggtcg agagacagat cgcgaccgaa gcgagtggcg agcgcgtgac gccgctggtg
     3661 cgcgcgcgcc tggccgagcc cttcgaggcg ctgcgtgcca ggtcacgacg cgagcgcgtc
     3721 gcgctcgtcg cggtcggtga gccggcgcag tggcgagcgc gggtcgagtt ctgtcgcgca
     3781 tacttcgagg tcggtggctt cgaggtcgtc gagacgcccg gcacgcagga gctcgcgcag
     3841 gcggtgcaac agttcacgga gacgggggcg cgcgcggcgg tgatctgctc ggccgaggcg
     3901 gtgtatccga cgaccgttcc ggagctggtg ccgatgttgc gtgaggccgg cgcggcggca
     3961 gtgctcgtcg ccgggcgacc gaaggatcag gtcgaggcgt tgacggcggc gggggtcgac
     4021 ctgttcgtgc agctcggcgg cgacgcgccc gcgctgctcg ggtccctcgc acagcggtta
     4081 gaggtgcgct cgtgaagaat tttcccgatt tcgccagcct cgactacgac gcgacaccga
     4141 cgcccgcgcc ggcgcccttc gacgagcgcc cggtgtggcg cacgcccgag ggcatcgacc
     4201 tccacgcgag ctacggggcc gaggcgctcg cggacgtcga cccggacttc ctgcgcacgc
     4261 agccgggcct gccgccgtac acgcgcgggc cgtacgcgac gatgtacacc cagcgcccgt
     4321 ggacgatccg gcaatacgct gggttctcga cggccgagga gagcaacgcg ttttatcgcc
     4381 gcaacctggc cggcggacaa aagggcctgt cgatcgcctt cgacctcgcg acgcaccgcg
     4441 gctacgactc cgatcatccc cgcgtggtcg gtgacgtcgg catggccggc gtggcgatcg
     4501 actcgatcct cgacatgcgg atccttttta atgggatccc gctcgaccag atgagcgtgt
     4561 cgatgacgat gaacggcgcg gtgctgccga tcctcgcgct ctacatcgtc gccgcggagg
     4621 agcagggcgt caagccggcc cagctccagg gcacgatcca gaacgacatt ttaaaagagt
     4681 tcatggtccg caatacgtat atttatccgc ccgcgccgtc gatgcgcatc gtggcggata
     4741 tcttcgcgta taccgcggag cacatgccaa agttcaacag catcagcatc tccggctatc
     4801 acatgcagga ggccggtgcg acggcggacc tcgagctggc ttatacgctg gcggacggcg
     4861 ccgagtacct gcgcaccggc gtcgccgcgg gcatggcgat cgacagcttc gcgccgcgcc
     4921 tgagcttctt ttggggcgtc ggcatgaact tcttcatgga ggtcgccaag ctgcgagcgg
     4981 cgcggctgct gtggtcgcgc atcgtcgccc gcttcggcgc gaagaacccc aagagcctgg
     5041 cgctgcgcac ccactgtcag acgtcgggct ggagcctcgc cgcgcaggac ccttataaca
     5101 atgtcgcgcg gacctgcgtc gaggcgctgg cggcgacgct cggcgggacg cagtcgctgc
     5161 ataccaatgc attcgacgag gcgctcgcgc tgccgaccga cttctcggcc cgcatcgccc
     5221 gcaacacgca gatctattta caagaggaga ccaacatcgg ccgcgtgatc gacccgtggg
     5281 gcgggagcta ccacgtcgag cgcctgacct gggacctcgc cgagaaggcg tgggcgctga
     5341 tcgaggaggt cgaggcgctc ggtggcatgg cgaaggcgat cgagcagggc ctccccaagc
     5401 tgcgcatcga ggaggccgcc gcgcgtacgc aggggcgcat cgacagcggc gcgcagacgg
     5461 tcgtcggcgt caatcgctat cggctcgccg aggaggcgcc gctcgacgtg ctcaaggtcg
     5521 acaacacggc ggtgcgcacg gcccagctgg cccggctggc ccagctgcga cgcgagcgcg
     5581 acagcacgga agtgaggcac acgctcgcgg cgctgacccg ctgcgccggc ggtgagtcgg
     5641 gcaacctgct cgcgctcgcg gtcgaggcgg cgcgcgcgag ggcgacggtc ggcgagatca
     5701 gcctcgcgct cgagcgtgtg tggggccgac atcaggcgac gacacgcacc gtgcgtggcg
     5761 tgtacagttc ggccgtgagc gacgtggggt cgatcgaggc ggtgcaggcg ctggcgcgcg
     5821 cgttctcgga ggcggaaggt cgacgaccgc ggttgctggt cgcgaagatg gggcaggacg
     5881 ggcacgaccg cggcgccaag gtgatcgcca ccgccttcgc ggacatgggc ttcgacgtcg
     5941 acatcggccc gctgttccag acgccggccg agacggcgct gcaggcggtc gagaacgacg
     6001 tgcacgtggt gggggtgtcg tccctcgcgg ccggacacct gaccctcgtg ccgcagctac
     6061 aaagcgagct gcggcgactc gggcgcgccg acatcatgat cgtggtcggc ggcgtgatcc
     6121 cgccgcacga tcacgagacg ctgcggacga tgggcgtggc ggcggtgttc ggacccggca
     6181 cggtgctgac gaccgcggcg gagcagatcg atcgcacgct cgcgcagcag ctcgagctcg
     6241 acctcggcgt cggttgacag cgatcaccgt tcgcgtaccc tgcgcgcacg atgagcctgt
     6301 ctgtcggcat cgatctcggg acgacgcgct gcgcggtggg cgtcatggag gccggcaagg
     6361 cgacgacgct gctcgtcaat cgccagccct accgcgaacc ctcggtgatc gggcacgagg
     6421 aggacgggtc gtcggtcatc ggcgagcgcg cgcgccggct cgccctgacg cgcccgcgcg
     6481 agatgatgct cgcggcccgt cgcctgctcg gccgccgctt cgacgaagtc gacgccgagg
     6541 cctggcgcgc acaaccactg ctgcgcgccg agaacggtga tgtgcaggtc aagctcggcg
     6601 agcacacggt ctcgacaccc gagctgctgg cgccgctgct gcgccaggtc cgacagacga
     6661 ccgaggccca gctcggcgag acgatcccga cggtcgtggt ggcggtgcgc gggcaacttc
     6721 gtgacctgca acgccgcgcg acgctggcgg cgtgccggct cggcgggctc gaaccgcgac
     6781 gactgatcca cacgacgacg gcggcggcgc tcgtgtatca cgcgacgcgc aacagcgtcg
     6841 gcgacgagcg gatcgccgtg atcgacttcg gcggcggcgg cttcgacgtc gcgctgatcg
     6901 aggtcggaga tcgcagcgtc gaggtgctcg cgcagcgcgg ggtcgaggtc ggcggcgagg
     6961 acctcgacgc gcgcatgatc gagtggctgc tcgccgagct gcgcgagcag acgggcatcg
     7021 acggcgcggc ggacccgatc gtgcgcgcgc gcatgcgtga cgcggccgag aaggcccgca
     7081 tcgcgctgtc ggagcagaag gaggtcgtcg tgcacctgcc gtacctcgcg gcggacgagg
     7141 ccggcccgaa gcacctcgcg tccaagctga cgcagaagcg cttcgaaaag ctggtcgccg
     7201 acctgctcga ccgctgcgcg gcgaccgtgc agcgcgcgct cgacgaggcg gggcggccga
     7261 tcgaccagtt cacgcaggtg ctgttcatcg gcgggtgtac gcgcatgccg gcgctgcacg
     7321 agcgcgtcgc gacgattttt aagaaggagc cgagcgcggc gctcgacagt gacgatgtcg
     7381 tcgcgcgtgg ggctgcgatc ttcgccggcc tgctggcgtc gcgcagcccg gagctggtgg
     7441 tccacgaggc gctgtcgcgg ccgctgtggt tgcaggtcgg ccgggccgag ccgcggccgc
     7501 tgttcccccc gcgctcgccg gtgccgaccg agcacagcga gccgctcgag acgccgggtg
     7561 acggccgtcc aggggtcgag tgtcgagtgt tggaaggcga gcgcgccggc ggcgaggcgc
     7621 cgctcctatt aaagtttacg gtcgcggggc ggccggaggt cgagacgaag ttcacgctcg
     7681 acggtaacca ggtcctcgac atcgcgatcc gcgagatgtt ccgcggcaag gaggcgcggc
     7741 tggtcatcga ggagcgcggt ggtctcgagg gacctgtgct cgcggcgatg atcgaggcgg
     7801 cgcgcgccga ggagaccgag gcgagggcgc aaagggcgat cgccgagcga cgccagcggc
     7861 tcgagggcat ggcccagcgc gccgagcggg tcacggccga gatggagaag ctgtcgcccg
     7921 agctgcgcgc ggccgtgacg gcgggggtcc gcgacgcgcg ggcagtgctg cagacgcagg
     7981 acgacgagct ggtgaccgcg gcgatcgagg ccttcgcggg gctgctgcgg ccgctgccgg
     8041 gcgagctcgc ggcgatcgct gcgccgccgg ccccgaccgg gcgcctgcaa ccggtgccga
     8101 tggctccgcc gaagcgcgtg gtggcgagcg acggcggcga gttcatcccc gacgaccccg
     8161 accagtcctg agtcagttcg agaggatccg gagctcgagg cggttgtttt tcaatgggcc
     8221 gtccggggcg tcgttgtcgg cgatcggctg ctcgctgccc tcggcgcgaa agtggatccg
     8281 tttggtcttg atcttctgct tctcgaccag gaacttgcgc gccgcgagca cacggcgggc
     8341 gctggggtcc tgcttgccgc gcagcttcca cgcgctgtcg tgatggccga cgatctcgat
     8401 ccgcagctcc gggtactcgc gcatgacggc ggcgatctgc tcgaggccgg ccttgatcgc
     8461 gggggtcagc tcgtccatcg ggcgcttgcg cagctcgtcg acgccgaggc cgaagaagat
     8521 cggccgatcg aggatcgccc aggccgccgg gaactcgtcg gggcagccgt cgtgatcgtg
     8581 aaagcggttg acggcctcgg gcgcgtcggg acagtcgtcg tcgacgtcga agatctggtc
     8641 gcggtcgctg tcgcgcgccg gacaaccatc aggctcgtgg ccgggctcgc cggggcaggt
     8701 gtcctggagg tcgaggaagc cgtcgctgtc gcggtcggcg ttggggtcga cggtgacgtc
     8761 ggcgagcatc gggccgccgc cgctggccat cgccgggccg ggccccgtgc gggcacgcgt
     8821 ggcgcaggcg ccgaggccga gcagcaggat cagggggagg cacttccctc gcacctgtcc
     8881 cctgtatcag atccggttca ggaccgcgaa agagggcgcg aacggagggc ggtgtccgca
     8941 gcggcgtcga agaagcgcag ccacagccag acgagggcgg tcgccgcgag caccgccccg
     9001 aggccaaagg cccacgcgag cccacgcccg gccgcatagc cgcccgccac cgagccgagc
     9061 aggcgcgcga cgccgaagca gaccatcgcg tagatgccct gcacgctgtt gcgcgcgccg
     9121 gcgggcgcct tgaggttgag gtacatcggc gggatcaggt agatggcgac gacgatcggg
     9181 ccgtgcaaca gctgcgaggc gacaatcacg cgcgggtcgg cgacgctcgc cagcaacacc
     9241 atgcgcacga ccaggcacag ggccccgaag actattagct ggcgcagggt gtagcggcgc
     9301 agcagagggc cggccgcgag catgaagggc agctcggaga cgacgccgag gttcatcacc
     9361 aggccgaccc acgacgggtc gatgccgacc tcgagcacga gccgcgcgta aaacccgtag
     9421 aacacggcga tcgaggcgaa cagcaggccg agcgggatga tgaggtcacg cgtcggcggc
     9481 cggcgcagca cgtcccacgc ggtcgtggag ggaggagttg taaggctggc ctcggggaca
     9541 ggtgcgccgg ggacgagggc gccggggacg agggcgccgg cgaggctggt ctcggggaca
     9601 ggtgcgccgg ggatgagcgc acccgcgagg ctggtctcct ggacaggtgc accggggatg
     9661 agcgcacccg cgaggctggt cgccgggaca ggtgcatccg cgaggctggt ctcagggaca
     9721 ggtgcgccgg ggtcgaggac gagggcgccg gggaggctgg tctccgggac aggtgcgccg
     9781 gggaggctgg tctccgggcc ggggcggccg ggggcgatgc gggggagcag ggggaggcac
     9841 agcaggccga ggcccgcggc gacggcggcg gcgaagatcg cggcgcgtcc ggaggcggcg
     9901 ccggctcgca gggcgaagaa caggacgaac gcggggatca tgaagccgaa gctgccccac
     9961 acgcggatgc ggtcgtaggg cggcacgggg cggccctcgg cctgcgcggc ggcgctggcc
    10021 gagaaggtga ggccgtcgag cagcgcgaac atgggggtat agaccatgct aaagaccatc
    10081 gagatcagca gcacggggat gaagccgcgg gcgaacgcga gggcgagcag ggtgcagccg
    10141 gtgagcccgt aggcgaggcc gatcagcgtg cggccggaca ggcggcggtc ggcgaggtag
    10201 gtgacgatcg gcgggctgag gatcaccgcc atgccgtaca tgccggcgat ccagccgatc
    10261 tgccagtcgg gcatgccgag gtcgcggccg aggaagaccg gcaggtacgg catgtacgcg
    10321 ccgacgatcg cgtacgcgag caggtaatgc aggcggatcg gaccgaagcg cacggggctt
    10381 caggactcgc gcggacgaaa gatgtgctgc cggtagtaac gcagctcggc gatcgactcg
    10441 cggatgtcgt cgagggcgcg gtgcgtctcg cccttctccg gcgccggcag ctcgggatac
    10501 cagcggcgcg cgagctcttt gatggaggtg acgtcgatgc tgcggtagtg caggaacgac
    10561 gcgagcaccg gcatgtgttt aattaagaat cggcggtcgt ggccgatcga gttgccgcac
    10621 agcggcgact tgccgggcgg gcagtgctgg cggatgaact cgaaggtctg ggtctcggcc
    10681 tggatgagcg tcgtcgtcga ggccttgacc tgcgcggtca ggccgctcag cgcatgctgg
    10741 cgggtgcacc aggcgtccat cgcgtcgagc acggggtcgg gctgatgcac gacgatgtcg
    10801 ggcccctcgg cgagcaggtt gaggtcacca tcggtgatga tggtggcgat ctcgaggat
//
LOCUS       DASWNJ010001386         2325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_376511, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001386 DASWNJ010000000
VERSION     DASWNJ010001386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376511"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..124)
                     /locus_tag="VGB85_23105"
     CDS             complement(<1..124)
                     /locus_tag="VGB85_23105"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEY0136998.1"
                     /translation="MLGAMLGGLEIAIIILVILLIFGPSKLPQLGEGIGKMLRGF"
     gene            220..2235
                     /locus_tag="VGB85_23110"
     CDS             220..2235
                     /locus_tag="VGB85_23110"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY0136999.1"
                     /translation="MGLGLLAPAGPVRAGEEARERVGPLMVSSSGRDSVRGGRPDGAT
                     IHETHAPRAHDPESGPREDLANFERQNFPIGTRPTIVASPPEPWMAALTLPDLPVRWN
                     QQTVAYLKYFRDDPRGQALIKGWIRRMNRYEHVLRPILKEVGVPEDLVFVAMAESGFN
                     PKVRSRVGAAGMWQFMEGTGRVYGLERDYWIDERHDLERATYAAATYLKDLRVRFGSW
                     EMALAAFNAGYGLVMTSVSRGNTNNFWALCQQESGLPHATTMYVPKIIAAAIVGRNRA
                     AFHVGPDLSGSLPPIHLATVEAPPATALTSVARAIGEDPAVIEELNAHLIRKRTPPGK
                     RTALRIPKQKLALFQQSAGRLGGDGGGEGSQHSVRYGETLAEIAQRYGTTEAALRKLN
                     ELDDTAELERDTVLLVPTTRSKTDAAPRPRPRAAVPLVTPDQGSRLVFFEVTRATSPR
                     GLGEAFGVAWDSIVAWNDLDPQARLQPGQILQIIVPRNFNAETARVDLYEADQVDRVV
                     RGSREHIEGMLRDRGLVRRGYRVRKGEDLTKIGKKFDLSDGDLGRINAFSRDHIPEPG
                     STIVVYVAKGKERGTVDAPPPVRAEAEAAAEPVPEAEHPRSPADMDAEPGPAHAPVEI
                     DAGASTETTTKVPGQQGWTRKPSTLGKTDGKSDSKPKKKAKKSGGAP"
BASE COUNT          380 a          869 c          799 g          277 t
ORIGIN      
        1 ggaagccccg cagcatcttg ccgatgccct ccccgagctg cggcagcttc gagggcccga
       61 agatgagcaa gatgaccagg atgatgatgg cgatctcaag accgccgagc atggcgccga
      121 gcatggctga aaggtagtcc ttcgggcccc gctttgctag cgtcgccacg aatgcccgcc
      181 gccttgcgcc gaagccgcga acacctcctg ctcgccctcg tgggcctggg gctgctggcg
      241 ccggccggtc cggtgcgcgc tggcgaggag gcgcgcgagc gggtcggccc gctgatggtc
      301 tcgagcagcg gccgcgactc ggtccgcggc ggacgcccgg atggcgcgac gatccacgag
      361 acccacgcgc cgagggctca cgaccccgag tcaggcccgc gcgaggacct ggcgaacttc
      421 gagcgccaga acttcccgat cggcacgcgc ccgacgatcg tcgcctcgcc gcccgagccg
      481 tggatggccg ccctgaccct gcccgacctg ccggtgcgct ggaaccagca gaccgtcgcg
      541 tacctcaagt acttccgcga cgacccgcgc ggccaggccc tgatcaaggg ctggatccgc
      601 cgcatgaacc gctacgagca cgtgctgcgc ccgatcctca aggaggtcgg cgtgcccgag
      661 gacctcgtgt tcgtggcgat ggccgagagc ggcttcaacc ccaaggtgcg atcgcgggtc
      721 ggcgccgccg gcatgtggca gttcatggag ggcaccggtc gcgtctacgg cctcgagcgc
      781 gactactgga tcgacgagcg ccacgacctc gagcgagcca cctacgccgc cgcaacttac
      841 ttgaaggacc tgcgcgtccg cttcggctcg tgggagatgg cgctcgccgc cttcaacgcc
      901 ggctacggcc tggtgatgac cagcgtctcg cgcggcaaca ccaacaactt ctgggcgctg
      961 tgtcagcagg agagcggcct gccgcacgcg acgacgatgt acgtgccgaa gatcatcgcc
     1021 gccgcgatcg tcggtcgcaa ccgcgcggcc ttccacgtcg gacctgacct ttcgggcagc
     1081 ttgccgccga tccacctcgc gaccgtcgag gccccgccgg cgaccgccct gaccagcgtc
     1141 gccagggcga tcggcgagga cccggcggtg atcgaggagc tcaacgccca cctgatccgc
     1201 aagcgcaccc cgccgggcaa gcgcaccgcc ctgcggatcc cgaagcagaa gctcgcgctg
     1261 tttcagcaga gcgccggccg gctcggcggc gacggcggcg gcgagggcag ccagcacagc
     1321 gtccgctacg gcgagaccct cgcggagatc gcccagcgat atggcacgac cgaggccgcg
     1381 ctgcgcaagc tcaacgagct cgacgacacc gccgagctcg agcgcgacac cgtcctgctg
     1441 gtcccgacca cccgcagcaa gaccgacgcg gccccgcgcc cgcgcccgcg cgccgcggtg
     1501 ccgctggtga cgccggacca gggctcgcgc ctggtgttct tcgaggtcac ccgcgcgacc
     1561 agcccgcgcg gcctgggcga ggccttcggg gtcgcgtggg acagcatcgt cgcgtggaac
     1621 gacctcgacc cgcaggctcg cctgcaaccg gggcagatcc tgcaaattat cgtgccgcgt
     1681 aatttcaacg ccgagacggc gcgcgtcgat ctgtacgagg ccgatcaggt cgaccgcgtg
     1741 gtccggggct cgcgcgagca catcgagggc atgctgcgcg accgcggcct ggtgcgtcgc
     1801 ggctaccgcg tgcgcaaggg cgaggacctg accaagatcg gcaagaagtt cgacctcagc
     1861 gacggcgacc tcggccgcat caacgcgttt agccgcgatc acatccccga gcccggcagc
     1921 acgatcgtcg tctacgtcgc caagggcaag gagcgcggca ccgtcgacgc cccgccgccg
     1981 gtgcgcgccg aggccgaggc cgccgccgag ccggtccccg aggccgagca cccgcgcagc
     2041 cccgctgaca tggacgccga gcccggcccc gcgcacgcgc ccgtcgagat cgacgccggc
     2101 gcctcgaccg agacgaccac caaggtgccc ggccagcagg gctggacccg caagccgagc
     2161 acgctcggca agaccgacgg caagagcgac agcaagccca agaagaaggc caagaagtcc
     2221 ggaggtgccc cgtgatcgcg agcgagctcg acatcgccgc ggcgcagcgc gaggcgctcg
     2281 cgcgggtgcg gccgctggcg actgtcgcgg ccgacctcgg cgacg
//
LOCUS       DASWNJ010001387         2435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_694811, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001387 DASWNJ010000000
VERSION     DASWNJ010001387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694811"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VGB85_23115"
     CDS             <1..296
                     /locus_tag="VGB85_23115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137000.1"
                     /translation="SLMNFMAPTADPTRPPPRAALKPEEKAVVALLTAAAEKLRKAAR
                     AKPAAKPPAKVTRPKAARPKAARPKAVPAPTPLKRPTRPRAEPTRKPTTRRAA"
     gene            372..1082
                     /locus_tag="VGB85_23120"
     CDS             372..1082
                     /locus_tag="VGB85_23120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137001.1"
                     /translation="MNSATKRLQHLAIISTATMILGCDQAPDGEPMVESEDMDKPQVL
                     SPRWVLRDKDGARMPALVEPRCGHWHDSQTQLHCDPPEFGSPGRFPCALVSFWEGQYI
                     NLQYELKSGTFGPCMSGQAPAEGNSSLRELAGFGFHYTDDACEGSLFKEMLGGGGGNY
                     GSDVSRHSRDVFRAVGKDWYISSEDCIDPGPCRIWLPSTSECQQDTCERLCPLKPVPQ
                     WLYELMPNPPYTMAVEYE"
     gene            complement(1168..2199)
                     /locus_tag="VGB85_23125"
     CDS             complement(1168..2199)
                     /locus_tag="VGB85_23125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137002.1"
                     /translation="MLADSPSRPVAWLAALALIAGACGGEVAAPAPVPQEFDDPFAEA
                     DGKAPVPEDSPVQAVAPAGGPVTAQVGLGAGGGAEVGPGAVGVAGAEVGSGAGEAGGA
                     GEVVGPAAGEAAVVGADAKAGGADVNAGGVVAGEKKPVKKAATPTEKPAPVPKDSPAP
                     VPEPAPVPEPTPTPAPAPTPVPVEKPAPPAVPPQQRFAGTYRYAGGEAQRQGLDAAIE
                     AAASELNALIRGIGRKRLKESNPVREQITIAVDGDKVSMTFEPGRTVTGRLEGPSVPW
                     TSDSGKPVQVNFSMVKGRLVQTFKAEDGGRRSVYSLDDTGDRLTLSVTITSERLTNPL
                     KYALTYRRS"
BASE COUNT          396 a          956 c          743 g          340 t
ORIGIN      
        1 gcagcctgat gaacttcatg gccccgaccg ccgacccgac gcgcccccca ccgcgcgccg
       61 ccctcaaacc tgaggagaag gccgtcgtcg ccctgctgac cgccgccgcc gaaaaactgc
      121 gcaaggcggc ccgcgcgaag cctgccgcaa aaccgcccgc caaggtcacc cgcccgaaag
      181 ccgcgcgccc gaaggccgcg cgcccgaaag ccgtgcccgc cccgaccccg ctcaagcgcc
      241 cgacccgacc gcgcgccgag ccgacccgca aaccgaccac ccgccgcgcc gcctgatccc
      301 tccaggtcgc ttccatcgcg taaaatggat accctgcccc gatagtcgcc gttttggtga
      361 ggtcagggtc gatgaacagc gcaacaaaaa gacttcaaca tctcgccatc atctcgacgg
      421 cgacaatgat cctcggctgc gatcaagcgc cggatggcga acccatggtc gagtccgagg
      481 acatggataa accgcaggtg ctctcccctc gctgggtgct gcgcgacaag gatggagccc
      541 gcatgcccgc gctcgtggag cctcgctgcg ggcattggca cgactcacag acgcagcttc
      601 attgcgatcc tcccgagttc ggctcacccg gccgctttcc atgtgcgctc gtgagctttt
      661 gggagggcca atatatcaac ctccagtacg agctcaagag tggcaccttc gggccctgca
      721 tgagcggcca ggcccccgca gagggcaaca gcagccttcg agaactcgcg ggcttcggat
      781 ttcattacac ggacgacgcc tgcgaagggt ctctttttaa agagatgctc ggaggcggag
      841 gaggcaatta cggctcggac gtctcccgac actcgagaga cgtcttcaga gcggtgggaa
      901 aggactggta catctcctcc gaggactgca tcgatccagg cccctgccgg atatggttgc
      961 cgagcacgag cgagtgccag caagacactt gcgagcgtct atgccccctg aaaccggtcc
     1021 cccagtggct ctacgagctc atgccgaatc ccccatacac aatggcggtg gagtacgaat
     1081 agagcccctg cggacctcgt ggatgctcgc gcggaccacg acgcggaccg cgccgcccga
     1141 gctcccgcga gactcacggc tccgcgatca actgcgacga tacgtcagcg catacttgag
     1201 cggattggtg agccgctcgg acgtgatcgt cacgctcaag gtcaggcgat cgccggtatc
     1261 gtcgaggctg tacacgctgc gccggccgcc gtcctccgcc ttgaaagtct ggaccagccg
     1321 gcccttgacc attgaaaaat tgacctgcac cggcttgccg ctgtcgctcg tccacggcac
     1381 cgacggcccc tcgagccgcc cggtcaccgt gcgacctggc tcgaaggtca tactgacctt
     1441 gtcgccgtcg accgcgatcg tgatctgctc gcgcaccggg ttgctttcct tgagccgctt
     1501 gcgcccgatc ccgcggatca gcgcgttgag ctcgctcgcc gccgcttcga tcgccgcgtc
     1561 gaggccttgc cgctgcgcct ccccgcccgc gtaacgatag gtgccagcga accgctgctg
     1621 cggcgggacc gccggcggcg cgggcttctc gaccggcaca ggcgtcggcg ccggtgcagg
     1681 cgtcggcgtc ggctcgggca ccggcgcagg ctcgggcacc ggcgcggggc tgtccttcgg
     1741 gacaggcgcc ggcttctcgg tcggagtcgc cgccttcttg accggcttct tctcgccggc
     1801 cacgaccccg cccgcgttca catccgcacc gccggccttc gcgtccgccc cgacgaccgc
     1861 agcctcgccc gccgccggcc cgaccacctc gcccgcgccc ccggcctcac ccgcgcccga
     1921 cccgacctcc gcgccggcca ccccgaccgc gcccggcccg acctccgcgc cgccccccgc
     1981 gcccagcccg acctgcgccg tcaccgggcc gccagccggc gcgaccgcct gcaccgggct
     2041 gtcctccggg acaggcgcct tgccgtccgc ctccgcgaac ggatcgtcga actcctgggg
     2101 caccggcgcc ggggcggcga cctcacctcc acatgcgccg gcgatcaacg cgagcgcggc
     2161 gagccacgcg acaggacggg acggggagtc ggcgagcacg tccggacagt acagcccgcc
     2221 gcggccgcga tctaccgatg tacaggtcgc tgatcgcccg cacgggctcc agggtcggcc
     2281 cacgcgggca tcgacgggcg ggcgcgggat cacgctataa tcgcgcggtc gccccgccga
     2341 ccgcccacca accccgaccg ccatgaccaa cccgaagctc ctgcaagaca ccatcgactt
     2401 cgacgccccg cccggcaccg gcgacccggc cgtgc
//
LOCUS       DASWNJ010001388         4904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_661403, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001388 DASWNJ010000000
VERSION     DASWNJ010001388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661403"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VGB85_23130"
     CDS             complement(<1..363)
                     /locus_tag="VGB85_23130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137003.1"
                     /translation="MASPGKIRPAAHLSRGTVGRAIAAALALASLTPSVATAAEYETF
                     VDIDDEDDLVELNAAGDISEETFDTLVELLRRGVNLNAARREELYTLPNLSYAEVDAI
                     IRYRTEVGFISDPAMLVTA"
     gene            complement(373..1314)
                     /locus_tag="VGB85_23135"
     CDS             complement(373..1314)
                     /locus_tag="VGB85_23135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY0137004.1"
                     /translation="MRRDRAPSDLSKPAGLAALALLLACCVSASVAAPSAAEARPAAA
                     ASATKSGHRPGWVAPQQARYQPQALAQISERVTAERATGKTPVVVFDLDHTLYDGRPR
                     TLVILAEFAATLPPAQARHAAAIQSIPLRGVAYLLKDTLAAAGVNDPQVVAAAEAYWK
                     PRFFSDAYVDLDVPLPGAAAYVQDLWRRGALVVYLSGRNAEAMLTGTTASLQKWGFPV
                     GVRGAQLILKPTFAEPDETFKQASLDHITSLGSVVASFENEPGNLAAMATRWPAAAHV
                     FLDTDFNPGGAVHTPPASARWVGDYSGYAPPAAPARP"
     gene            1382..1900
                     /locus_tag="VGB85_23140"
     CDS             1382..1900
                     /locus_tag="VGB85_23140"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEY0137005.1"
                     /translation="MTRRETACVLVLVLCGGCPAAALLPVTPAMDPMGEQERDDDDCV
                     LDSWEDGDTAHVDCGSGAMEVVRLQGIEAAETGTNPESKTRAKWQADLWKLPYEVVIR
                     CGEAAVARAKEICPEGSTVLLVGDDTDKLDRRLAHVRCAGQNVNVRLLQEGHAGHHAE
                     PADPARPHRCHM"
     gene            complement(1950..2450)
                     /locus_tag="VGB85_23145"
     CDS             complement(1950..2450)
                     /locus_tag="VGB85_23145"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEY0137006.1"
                     /translation="MSVTPRCAILLSLVACAPDAQTSTDPISLCGPSKATVASVIDGD
                     TIVLDTGEKVRYLMIDTPEITSGKHDCYGEEARDYNEELVLGQEVSLTYDVECSDRYG
                     RLLAYVEAPDGEMNTLLLERGFACLLVLPPNGAERESEFATLALEARQSGEGMWTACE
                     GAVACD"
     gene            complement(2441..>4904)
                     /locus_tag="VGB85_23150"
     CDS             complement(2441..>4904)
                     /locus_tag="VGB85_23150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137007.1"
                     /translation="LGTKPPDAISNDDLTAWCLASEAYAGKDFGSPGAQNPDCPLPEA
                     PCGQCYDGELLRPTKTPQPGQLVITEIMPNASTKTDATVGEWFEVLVTEGSVDLNCLQ
                     FGGNVTKFLTDTSDPEQVLNTSECLNYNAGTYLVFAEHPAGVDADFESKISLVDSASD
                     SNPTPGVYLAYDGRVLDEAHYTKTMDGIAWSLDPDALTTLGNDDPLNWCGASNPFAEG
                     DLGSPGESNPQCPEAVKEGTCVDGDGSKRDIDYAAPGELLITEFLTDPALGDGTLGEW
                     IELYVGADLDLNGMTLGKSLESPYATISDVACLPIPADTYVLIAKSADPAENGGLPVV
                     DWWNPKISLTNDGSNLVVGVENKLTMTSAELDATSWQTTADGKARQFPAQLVPAAVPF
                     DTTINDDPTQWCDASEPFGAGDLGTPRAENLSCDVAPPMDQCTDPVTMMLRPIVHPVA
                     GDLLLTEIHPDPDALIKSGGAGEPTGEWFELYAATAFDLNGLELGNTFPTKKHTVTSD
                     LCLPVAADSYALLAQTGKPLSPPNPDDLAGNGGLPTPNYTYTGLSLSNTGGSLYVGFA
                     DVALDTITSYPKPAIGKATQLGEEAMCITAAPLDTACNDDFAKWCAASTAYGLGDFGT
                     PQAANVACGGGNMEATCFDDALQMMRPIVAPKPGDLVINEFLSDPTVVTDANGEWFEL
                     AVLADVDLNDLKIINKPNVDMATLAAAKPLLVSPDCLRATAGSFVLFAHNADPAINGG
                     LPAVDHVVATSLGNASGGISIGLGDVLLHTVGWALAQKAGKSAALDPDGKADPMNISA
                     DGPPWCTSLDAGTPKQENPQCP"
BASE COUNT          640 a         1475 c         2006 g          783 t
ORIGIN      
        1 ggcggtgacc agcatcgcgg ggtcggagat gaagccgacc tcggtgcgat agcggatgat
       61 cgcgtcgacc tcggcgtagc tgaggttggg cagggtgtac agctcttcgc ggcgggccgc
      121 gttgaggttg acgccgcggc gcagcagctc gacgagggtg tcgaaggtct cctcggagat
      181 gtcaccggcg gcgttgagct cgacgaggtc gtcctcgtcg tcgatgtcga cgaaggtctc
      241 gtactcggcg gcggtggcga cgctcggcgt gaggctggcg agcgcgagcg ctgcggcgat
      301 cgcccgaccg actgtcccgc gggacaggtg agcggcgggg cggatcttgc cggggctggc
      361 catcaggtag ggtcaggggc gcgccggggc ggcgggcggc gcatagccgg agtagtcgcc
      421 gacccagcgg gccgaggcgg ggggcgtgtg cacggcgccg ccggggttga agtcggtgtc
      481 gaggaagacg tgcgcggcgg cgggccagcg ggtcgccatc gcggcgaggt tgccgggctc
      541 gttctcgaag ctggcgacga cgctgccgag cgaggtgatg tgatcgaggg aggcctgctt
      601 gaaggtctcg tcgggctcgg cgaaggtcgg cttgaggatc agctgggctc cgcgcacgcc
      661 gaccgggaag ccccactttt gcagcgaggc ggtggtgccg gtcagcatcg cctcggcgtt
      721 gcgccccgag aggtagacga cgagcgcgcc gcggcgccac aggtcctgca cgtaggcggc
      781 ggcgccgggc agcgggacgt cgaggtcgac gtaggcgtcg ctgaagaagc gcggcttcca
      841 gtaggcctcg gcggcggcga cgacctgggg gtcgttcacg ccggcggcgg cgagggtgtc
      901 cttgagcagg taggcgacgc cgcgcagcgg gatgctctgg atcgcggcgg cgtggcgggc
      961 ctgcgccggc ggcaaggtgg ccgcgaactc ggcgaggatg acgagggtgc gcgggcggcc
     1021 gtcatacagg gtgtggtcga ggtcgaagac gacgacgggg gtcttcccgg tggcccgctc
     1081 ggcggtgacg cgctcgctga tctgcgcgag cgcctgcggt tgatacctgg cctgctgcgg
     1141 cgcgacccag cccggccgat ggccggactt cgtcgcgctg gcggccgcgg cgggcctggc
     1201 ctcggcggcg ctgggcgcgg ccacggaggc gctgacgcag caggccagca gcagcgcgag
     1261 cgcggcgagg cccgcgggtt tcgacaggtc ggacggggcg cggtcccttc gcacgcgggc
     1321 cagtgtgccg aaaccggcgc gcgcttgcca gcgcggaggc gaccggacat gctggtcgca
     1381 cgtgacgcgg cgtgagacag cctgtgtgct cgtgctggtg ctgtgcggcg gctgcccggc
     1441 ggcggcgctg ttgccggtga cgccggcgat ggacccgatg ggcgagcagg agcgcgacga
     1501 cgacgactgc gtgctcgaca gctgggagga cggcgacacg gcgcacgtgg actgtggatc
     1561 aggcgccatg gaggtggtcc gactgcaggg catcgaggcg gcggagacgg gcacgaaccc
     1621 agagtcgaag acgcgggcga agtggcaggc ggacctgtgg aagctgccct acgaggtggt
     1681 gatccgctgc ggcgaggcgg ccgtggcgcg ggcaaaggag atctgccccg agggttcgac
     1741 ggtgctgctc gtcggcgacg acaccgacaa gctcgatcgc cggctcgcgc atgtccgctg
     1801 cgccggacaa aacgtcaacg tgcgactgct gcaggagggc cacgcgggac accacgcgga
     1861 gccggccgac ccggcgcggc cgcaccgctg tcatatgtga tgccgccata tgtgatgccg
     1921 tccctcggga gcagcccgcg agcatcggct caatcacagg cgacggctcc ctcgcaggcg
     1981 gtccacatgc cctcgccgga ctggcgggcc tcgagcgcga gggtggcgaa ctcggactca
     2041 cgctcggcgc cgttgggcgg gagcacgagc aggcaggcga agccgcgctc gagcaggagg
     2101 gtgttcatct cgccgtcggg ggcctcgacg taggcgagca ggcggccgta cctgtcgctg
     2161 cactcgacgt cgtaggtgag gctgacctcc tggccgagca cgagctcctc gttgtagtcg
     2221 cgggcctcct cgccgtaaca gtcgtgcttg ccgctggtga tctccggggt gtcgatcatc
     2281 aggtagcgga ccttttcgcc ggtatcgagg acgatcgtgt cgccgtcgat cacggaggcg
     2341 acggtcgcct tgctcgggcc gcacaggctg atcgggtcag tgctcgtctg cgcgtccggg
     2401 gcgcaggcga cgagcgacag gaggatcgcg cagcggggcg tcacggacat tgcgggttct
     2461 cctgcttcgg ggtgccggcg tcgagggagg tgcaccacgg cgggccgtcg gcgctgatgt
     2521 tcatcgggtc ggccttgcca tcgggatcga gggcggccga cttgccggcc ttctgcgcga
     2581 gcgcccagcc gacggtgtgc agcaggacgt cgccgaggcc gatcgagatg ccgccgctgg
     2641 cgttgccgag gctggtggcg acgacgtggt cgaccgccgg caggccgccg ttgatcgcag
     2701 ggtcggcgtt gtgggcgaac agcacgaagc tgccggcggt ggcgcgcagg cagtcggggc
     2761 tgacgagcaa cggcttggcg gcggcgaggg tcgccatgtc gacgttgggc ttgttgatga
     2821 tcttgaggtc gttgaggtcg acgtcggcga gcacggcgag ctcgaaccac tcgccgttgg
     2881 cgtcggtgac gaccgtcggg tcggacagga actcgttgat gacgaggtca ccgggcttgg
     2941 gggcgacgat cgggcgcatc atctgcaggg cgtcgtcgaa gcaggtcgcc tccatgttgc
     3001 cgccgccgca ggcgacgttg gcggcctgcg gggtgccgaa gtcgccgagg ccgtaggcgg
     3061 tcgaggcggc gcaccacttg gcgaagtcgt cgttgcaggc ggtgtcgagc ggggcggcgg
     3121 tgatgcacat cgcctcctcg ccgagctggg tcgccttgcc gatcgccggc ttggggtagc
     3181 tggtgatcgt gtcgagggcg acgtcagcaa agccgacgta gaggctgccg ccggtgttgc
     3241 tcaggctcag gccggtgtac gtgtagttcg gcgtcggcag gccgccgttg ccggcgaggt
     3301 cgtcggggtt cggcggcgag agcggcttgc cggtctgggc gagcagcgcg tagctgtcgg
     3361 cggcgaccgg caggcacagg tcgctggtga cggtgtgctt cttggtcggg aaggtgttgc
     3421 cgagctcgag gccgttgagg tcgaaggcgg tggcggcgta gagctcgaac cactcgccgg
     3481 tcggctcacc ggcgccgccg ctcttgatca gggcgtcggg gtcggggtgg atctcggtca
     3541 gcagcaggtc gcccgcgacc gggtgcacga tcgggcgcag catcatcgtc accgggtcgg
     3601 tgcactggtc catcggcggg gcgacgtcgc agctgaggtt ctcggcgcgc ggggtgccga
     3661 ggtcgccggc gccgaacggc tcgctggcgt cgcaccactg ggtcgggtcg tcgttgatcg
     3721 tggtgtcgaa gggcacggcc gcgggcacca gctgggccgg gaactgccgc gccttgccgt
     3781 cggcggtggt ctgccacgag gtcgcgtcga gctcggcgct ggtcatcgtc agcttgttct
     3841 cgacgccgac gacgaggttg gagccgtcgt tggtgagcga gatcttgggg ttccaccagt
     3901 cgacgaccgg caggccacca ttttcggccg ggtccgcgct cttggcgatc agcacgtagg
     3961 tgtccgcggg gatcggcagg caggcgacgt cggagatcgt cgcgtacggg ctctcgagcg
     4021 acttgccgag ggtcatgccg ttgaggtcga ggtcggcgcc gacgtagagc tcgatccact
     4081 cgccgagggt gccgtcgccg agcgcggggt cggtgaggaa ctcggtgatc agcagctcgc
     4141 cgggggcggc gtagtcgatg tcgcgcttgc tgccgtcgcc gtcgacgcag gtgccctcct
     4201 ttacggcctc ggggcactgc gggttgctct cgccggggct gccgaggtcg ccctcggcga
     4261 aggggttgct cgcgccgcac cagttgagcg ggtcgtcgtt gccgagggtc gtcagggcgt
     4321 cggggtcgag gctccaggcg atgccgtcca tcgtcttggt gtagtgggcc tcgtcgagga
     4381 ccctgccgtc gtaagcgaga tagacgccgg gggtcgggtt cgagtccgag gcgctgtcga
     4441 cgaggctgat cttgctctcg aagtcggcgt cgacgcccgc ggggtgctcg gcgaacacga
     4501 ggtaggtgcc ggcgttgtag ttgaggcact cggaggtgtt gagcacctgc tcggggtcgg
     4561 aggtgtcggt gaggaacttg gtgacgttgc cgccgaactg cagacagttg aggtcgacgc
     4621 tgccctcggt gaccaggacc tcgaaccact cgccgacggt ggcgtcggtc ttggtgctcg
     4681 cgttgggcat gatctcggtg atcacgagct ggccgggctg cggggtcttg gtcgggcgca
     4741 gcagctcgcc gtcgtagcac tggccgcagg gggcctctgg caacgggcag tcggggttct
     4801 gcgcgccggg gctgccgaag tccttgccgg cgtaggcctc gctggcgagg caccaggcgg
     4861 tcaggtcgtc gttgctgatc gcgtcgggcg gcttggtgcc gagg
//
LOCUS       DASWNJ010001389         1586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_336955, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001389 DASWNJ010000000
VERSION     DASWNJ010001389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336955"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VGB85_23155"
     CDS             <1..602
                     /locus_tag="VGB85_23155"
                     /inference="COORDINATES: protein motif:HMM:NF026214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEY0137008.1"
                     /translation="GERWALLVVRELVLGPTRFTDLRAGLLAASPNVLSQRLRELEEH
                     GVVARRVLPRPASATVYELTAWGRELEPVILALGRWGSRAAQVPGGELSVDATMLALR
                     TTFDAEAAEGLRARIAVQLGSDEFRLHVARRRIEISRGSGDDCDATLTTDPATLRAVV
                     FGGRPVASAKKAGALAISGDEALVVKVLGLFVRPAPAAP"
     gene            complement(661..1296)
                     /locus_tag="VGB85_23160"
     CDS             complement(661..1296)
                     /locus_tag="VGB85_23160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137009.1"
                     /translation="MLTHACKLLVSFSLLLLGACDLKPEVIGATLPDAEGASTTGDPT
                     TGDPTSTTTEPTTGAPDGTPCQLGPPEAWEEGKMPEPGAPIVYDGGRIVTPGPDCAGD
                     VCLYAADVIPPDCEDDAACVGDPRLTGTCGEFRCDVDPAWGEAHTTCTRLCEVDTDCP
                     VIPGCVEGPVCNVITKLGSLCCQKICACRDELSEGSLEAVEFDCNQDMFCP"
     gene            complement(1304..>1586)
                     /locus_tag="VGB85_23165"
     CDS             complement(1304..>1586)
                     /locus_tag="VGB85_23165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137010.1"
                     /translation="ALLTQARGALRARDPASAQTLLERHAREFPDGALTDERRLSQIS
                     ALCLSGQVERARAEAVRLADERPTTAARAAALCPFFSENSATDAARPGD"
BASE COUNT          200 a          527 c          634 g          225 t
ORIGIN      
        1 tcggcgagcg gtgggcgctg ctcgtcgtgc gcgagctggt gctcgggccc acgcggttca
       61 cggatctgcg ggccgggttg ctggcggcga gcccgaacgt gctgtcacag cggctgcgcg
      121 agctcgagga gcacggcgtc gtcgcgcgcc gggtgctgcc gcggccggcg agtgccacgg
      181 tctacgagct gaccgcgtgg ggccgcgagc tcgagccggt gatcctcgcg ctcgggcgct
      241 ggggcagccg cgcggcgcag gtcccgggcg gcgagctcag cgtcgacgcg acgatgctcg
      301 cgctgcgcac gaccttcgac gccgaggcgg cggaggggct gcgggcgcgc atcgccgtgc
      361 agctcgggtc ggatgaattt cgcctgcatg tggctcgccg gcggatcgag atctcgcgcg
      421 ggtcgggcga cgactgtgac gcgaccctga ccaccgaccc cgcgacgctg cgcgcggtgg
      481 tgttcggcgg gcgtccggtc gccagcgcga agaaggcggg cgcgctggcg atctcgggcg
      541 acgaggcgct ggtcgtgaag gtgctcgggc tgttcgtccg accggcaccg gccgcgccct
      601 gagcgtcgtc atgacgcttc acagcgcgcg cagccgggcg tcgagccggg cggtggcgcg
      661 tcacgggcag aacatatctt ggttgcagtc gaactcgacc gcctcgagcg aaccctcgga
      721 gagctcgtcg cggcacgcgc agatcttctg gcagcatagc gagccgagct tggtgatcac
      781 gttgcacacg gggccctcga cgcagccggg gatcacgggg cagtcggtgt cgacctcgca
      841 caggcgggtg caagtcgtgt gcgcctcgcc ccacgccggg tcgacgtcgc accggaattc
      901 gccgcaggtg ccggtcaggc gcggatcacc gacgcaggcg gcgtcgtcct cacaatcggg
      961 cgggatgacg tccgcggcgt acaggcacac gtctccggcg cagtccgggc cgggcgtgac
     1021 gatccgaccg ccgtcgtaca cgatcggcgc gccgggctcg ggcatcttgc cctcctccca
     1081 ggcctccggc ggcccgagct ggcacggggt gccgtcgggc gcgccggtgg tcggctcggt
     1141 ggtggtcgag gtcggatcgc cggtggtcgg gtcgccggtg gtcgaggcgc cctcggcgtc
     1201 cgggagcgtc gcgccgatga cctcgggctt gaggtcgcag gcgccgagga gcagcaggga
     1261 gaaggagacg agcagcttgc atgcgtgagt gagcatgatc ctctcagtct ccgggacgcg
     1321 ccgcgtccgt cgcggagttt tctgaaaaga aagggcacag cgccgcggcc cgggcggcgg
     1381 tcgtggggcg ctcgtcggcg aggcgtacgg cctcggcgcg cgcgcgctcg acctgtcccg
     1441 agagacagag cgccgagatc tgcgacaatc ttcgctcgtc ggtcaaggcg ccgtcgggga
     1501 actcgcgggc gtgacgctcg agcagggttt gggcgctcgc cggatcgcgg gcacgcaggg
     1561 cgccacgcgc ctgggtgagc agcgcc
//
LOCUS       DASWNJ010001390         1851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_517758, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001390 DASWNJ010000000
VERSION     DASWNJ010001390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517758"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1851
                     /gene="glgX"
                     /locus_tag="VGB85_23170"
     CDS             <1..>1851
                     /gene="glgX"
                     /locus_tag="VGB85_23170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEY0137011.1"
                     /translation="SAVDLCLFDGVDAAPRETLRLADRTGDVHHAYVPGLRAGQLYGL
                     RAHGPHEPHAGLRHNPHKLLVDPYARSIAWPTRSGEIPYAHKVGDPAGELAIDTRDNA
                     AQAPKGVALVDDFDWGDDRPPARPWSDTLIYELHVKGMTKLHPGVPPALRGTYGGLAT
                     PPVIDHLRGLGVTAVELLPIHEIYDEPHLRNSGRVNYWGYSTLGFFAPSARYAADRRP
                     GAAVREFKAMVKALHAAGIEVILDVVYNHTCEGGALGPLLSLRGLDNRTYYTLEHDDP
                     RRYADVTGCGNTLNTRHPQTLQLVLDSLRYWATQMRVDGFRFDLAPALSRRGPEFDRW
                     SAFLAAVHQDPVLSRVKLIAEPWDVGPGGYQVGGFPAPWSEWNGRYRDGVRKFWRGES
                     SQLGELAYRLTGSADVYQWPRRGPHASVNFVTCHDGFTLRDLVTYQRKHNHGNGEHNR
                     DGSDHESSANWGVEGPTDDPEIVALRRRMIRNFATTLAVSLGVPMICAGDEIGRTQGG
                     NNNAYCQDSEVSWIAWDSADEATLAFFRHAMRMRQAAVLRRRGFFTGAGAPGSRMRDL
                     QWLHPDGHEPTPREWSDPQARAIGMLLGGDAIGEVDARGDVLHGESFLVLI"
BASE COUNT          276 a          669 c          661 g          245 t
ORIGIN      
        1 tcggcggtcg acctgtgcct gttcgacggc gtcgacgcgg cgccgcgcga gaccctgcgg
       61 ctcgcggatc gcaccggcga tgtccatcac gcgtacgtgc cgggtctgcg cgccggacag
      121 ctttatggcc tgcgcgcgca cgggccccac gagccgcacg ccgggctgcg ccacaacccg
      181 cacaagctgc tcgtcgaccc ctacgcgcgg tcgatcgcct ggccgacccg cagcggcgag
      241 atcccgtacg cgcacaaggt cggtgacccg gcgggcgagc tggcgatcga cacgcgggat
      301 aacgccgcgc aggcccccaa gggcgtcgcg ctggtcgacg acttcgactg gggcgacgac
      361 cgaccgcccg cgcgtccgtg gagcgacacg ctgatctacg agctgcacgt caagggcatg
      421 accaagctgc acccgggcgt gccgccggcc ctgcgcggga cctacggcgg gctggcgacg
      481 ccgccggtga tcgaccacct gcgcgggctc ggggtcaccg cggtcgagct gctgccgatc
      541 cacgagatct acgacgagcc gcacctgcgc aacagcgggc gcgtcaacta ctggggttac
      601 tcgacgctcg gcttcttcgc ccccagtgcc cgttacgcgg ccgaccgccg gcccggcgcc
      661 gcggtgcgag aattcaaggc gatggtcaag gcgctgcacg ccgccggcat cgaggtgatc
      721 ctcgacgtcg tctacaacca tacctgcgag ggcggggcgc tcgggccgtt gctgtcgctg
      781 cgcgggctcg acaaccgcac ctactacacg ctcgagcacg acgacccgcg ccgctacgcc
      841 gacgtcaccg gctgcggcaa cacgcttaac acgcggcatc cgcagacgct gcagctcgtg
      901 ctcgacagcc tgcgctactg ggcgacgcag atgcgcgtcg acgggtttcg cttcgacctc
      961 gcgccagcgc tgtcacgccg cgggccggag ttcgaccgct ggagcgcgtt cctggcggcg
     1021 gtgcatcagg acccggtgct gtcgcgcgtc aagctgatcg ccgagccgtg ggacgtcggg
     1081 ccgggtggat atcaggtcgg cggcttcccg gccccgtgga gcgagtggaa cggccgctac
     1141 cgcgacggcg tgcgcaagtt ctggcgcggc gagtcgagcc agctcggcga gctcgcgtat
     1201 cgcctcaccg gctcggccga cgtgtaccag tggccgcgcc ggggcccgca cgcgagcgtc
     1261 aacttcgtca cctgccacga cggcttcacg ctgcgcgacc tggtgacgta tcagcgcaag
     1321 cacaaccacg gcaacggcga gcacaaccgc gacggcagcg accacgagag cagcgccaac
     1381 tggggcgtcg aggggccgac cgacgacccg gagatcgtcg cgctgcggcg gcgaatgatc
     1441 cgcaacttcg cgacgacgct ggcggtgtca ctcggcgtgc cgatgatctg cgcgggcgac
     1501 gagatcgggc gcacgcaggg cggcaacaac aacgcttact gccaggacag cgaggtctcg
     1561 tggatcgcgt gggacagcgc cgacgaggcg acgctggcgt tctttcgcca cgcgatgcgc
     1621 atgcgccagg cggcggtgct gcgccggcgc ggcttcttca ccggcgcggg cgctccgggc
     1681 agccgcatgc gcgacctgca gtggctgcac cccgacggcc acgagccgac gccgcgcgag
     1741 tggtccgacc cgcaggcgcg cgcgatcggg atgctgctcg gcggcgacgc gatcggcgag
     1801 gtcgatgcgc gcggtgacgt cctgcacggc gagagcttcc tcgtgctgat c
//
LOCUS       DASWNJ010001391         2657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_25607, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001391 DASWNJ010000000
VERSION     DASWNJ010001391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25607"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            449..>2657
                     /locus_tag="VGB85_23175"
     CDS             449..>2657
                     /locus_tag="VGB85_23175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY0137012.1"
                     /translation="MPSADLNAQSPLQPAPAGPTPRYRAHNKIRVVTAAALFDGHDAA
                     IGVMRRVLQASGCEVIHLGHNRGAEEVVDAAIQEDAQAIALSSYQGGHVEYFQYMHDL
                     LRQKGAGHITIWGGGGGVIVPREIEKLHAYGINRIFSPEDGRRHGLQGMIDMIVSGAD
                     FSTTPDDPLRELAQVRGADARARAEHRSQLARLITAVEALPDGDSPLRDALRAGEVAG
                     VPVLGITGTGGAGKSSLTDELLSRLLRDHPERHIAVVSVDPTRRRTGGALLGDRIRMN
                     ALRNPRVYMRSLATRQAGVELSRATDDVVRLIKQAGYDLVIVETSGIGQGDAEITRLA
                     DLCVYVMTAEYGAPSQLEKIEMLDLAQLVAINKFEHKGSKDALRDVRKQFRRNREIPY
                     GVPDEALPVFGTVASRFFDSGVNGLYAAVCEGLTGHARAPWVCTIPVDQRAVASDTRD
                     LIPPERTRYLAEIVRTVRDYKARAHAQVQRVRELAALRTAKVLLAGESGVDAAAVDRA
                     IAAAELKVEADARRALAEIPRLRDAYAQDEYVVKIRNAEVVYPTHVVTLSGTRQPRVA
                     VPQDDEPGALVRFALLENLPGYFPFTAGVFPFKREGEDPKRMFAGEGGPSRTNERFHY
                     LSAGEPAKRLSTAFDSVTLYGQDPDERPDIFGKIGESGVSVCTLEDAHDLYAGFDLCD
                     PATSVSMTINGPAPIVLAFYMNTAIDQQVAAAEAKKGDKLSAEELAAVRDRTLK"
BASE COUNT          412 a          905 c          960 g          380 t
ORIGIN      
        1 gcgaggtcgc cggcgagatc ggccgtgttt cactgtcacc gcgacgcctg gccctcgcga
       61 catgtctctc gggacctgtc tcgacgcacc tggccttttg gacctgcgtc agaccccggg
      121 cgtgacgcgt gccctcgggg caggccggca ggcgggtgct cggcaggcgc cagcgcggcg
      181 cgctcgacca gccggggcgt ggccgtccgc tcgtcgcgtc cgctgcgccg ggggcagcga
      241 gcaccaccag aaacccgagg cacagcggca ggcaggccgc gaacgcccgc cagggccggt
      301 ttgcacggcc tccggcgacc ctggtaggtc gaggaaggtc cgtgtgcgtg ggtgtcatac
      361 cagcgacatt gtgagccaag ccgcagccgg aggcaaaaaa cgtgggtccc ggggtggcct
      421 tcgcgggcct actgtggctc tcagaacgat gcccagcgcc gatctgaacg cccagagccc
      481 cctccagccg gcgccggccg gtcccacgcc gcgctatcgc gcccacaaca agatccgcgt
      541 ggtgaccgcc gccgccctgt tcgacggcca cgacgccgcg atcggcgtga tgcgtcgcgt
      601 gctccaggcg tccgggtgcg aggtcattca tctcgggcac aaccgtggcg cggaggaggt
      661 cgtcgacgcg gcgatccagg aggacgcgca ggcgatcgcg ctgagctcct atcagggcgg
      721 gcacgtcgag tacttccagt acatgcacga tctcctgcgc caaaaagggg cggggcacat
      781 cacgatctgg ggcggtggcg gcggcgtgat cgtgccgcga gagatcgaga agctgcacgc
      841 ctacggcatc aatcgcatct ttagccccga ggacggccga cgccacgggc tgcagggcat
      901 gatcgacatg atcgtctcgg gggccgactt ttcgaccacg cccgacgacc cgctccgcga
      961 gctcgcgcag gtccgcggag ccgacgcgcg cgcgcgggcc gagcatcgca gccagctcgc
     1021 gcggctgatc acggcggtcg aggcgttgcc cgacggcgac tcccctttac gagatgcgtt
     1081 gcgggccggc gaggtcgcgg gtgtgccggt gctcggcatc accggcaccg gcggcgcggg
     1141 caagagctcg ctgacggacg agctgctcag tcgcctgttg cgtgatcatc ccgagcgtca
     1201 catcgcggtc gtgtcggtcg atccgacgcg gcggcgcacg ggcggcgcgc tgctcggcga
     1261 tcgcatccgc atgaacgcgc tgcgaaaccc gcgggtatac atgcgcagcc tcgccacgcg
     1321 gcaggccggc gtcgagctgt cgcgcgcgac cgacgacgtc gtgcgcttaa ttaagcaggc
     1381 gggctacgat ctggtgatcg tcgagacctc ggggatcggt cagggcgacg ccgagatcac
     1441 gcggctcgcg gatctttgtg tgtacgtgat gacggccgag tacggcgcgc cgagccagct
     1501 cgagaagatc gagatgctgg acctcgcgca gctggttgcg atcaacaagt tcgagcacaa
     1561 gggcagcaag gacgcgctcc gcgacgtccg caagcagttc cggcgcaatc gcgagatccc
     1621 gtacggcgtg cccgacgagg cgctgccggt gttcggcacg gtcgcgagtc ggttcttcga
     1681 cagcggcgtc aacgggctct acgccgccgt gtgcgagggc ctcacggggc acgcgcgggc
     1741 cccgtgggtg tgcaccattc ccgtcgacca gcgcgccgtg gcctcggata cgcgggacct
     1801 gatcccgccg gagcgcacgc gttacctcgc ggagatcgtc cgcacggtgc gtgactacaa
     1861 ggcccgcgcg cacgcgcagg tgcagcgggt ccgcgagctt gccgcgctgc gcaccgccaa
     1921 ggtgctgctc gccggcgagt cgggggtcga cgcggcggcg gtcgatcgtg cgatcgcggc
     1981 ggcggagctc aaggtcgagg ccgacgcccg tcgcgcgctc gccgagatcc cacgacttcg
     2041 tgacgcgtac gcgcaggacg agtacgtcgt gaagatccgc aacgcggagg tcgtgtatcc
     2101 gacgcacgtc gtcacgctct cgggcactcg ccagccgcgc gtcgccgtgc cgcaggacga
     2161 cgagccgggc gcgctggtcc gcttcgcgct gctcgagaac ctgccgggat acttcccgtt
     2221 caccgccggc gtgttcccgt tcaagcgcga gggtgaggat cccaagcgca tgttcgcggg
     2281 cgagggcggg ccgtcgcgca ccaacgaacg cttccattat ctgtcggcgg gcgagccggc
     2341 caagcgcctg tcgacggcct tcgattcggt gacgctgtac ggtcaggacc ccgacgagcg
     2401 ccccgatatc ttcggcaaga tcggcgagag cggcgtcagc gtgtgcacgc tggaggacgc
     2461 gcacgacctg tatgccggct tcgatctgtg cgacccggcg acgagcgtca gcatgacgat
     2521 caacggcccg gcgccgatcg tgctcgcgtt ctacatgaac acggcgatcg accaacaggt
     2581 cgcggcggcc gaggcgaaga agggcgacaa gctgagcgcg gaggagctcg cggcggtgcg
     2641 cgaccggacc ctcaagg
//
LOCUS       DASWNJ010001392         2704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_446819, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001392 DASWNJ010000000
VERSION     DASWNJ010001392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446819"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VGB85_23180"
     CDS             complement(<1..454)
                     /locus_tag="VGB85_23180"
                     /inference="COORDINATES: protein motif:HMM:NF015666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY0137013.1"
                     /translation="MDPVAPHLRLLQKLADHSAHIVVLGMGYVGLPLAVAFAEAGFVR
                     VTGLDIDASKLDALQRGESYIEDVAAERVRRVIGTRLFTSTDFEATLATADVAIVCVP
                     TPLNKTRDPDLRALTAASQAIARALHPGMLVSLESTTYPGTTDELLRPM"
     gene            complement(513..1529)
                     /locus_tag="VGB85_23185"
     CDS             complement(513..1529)
                     /locus_tag="VGB85_23185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0137014.1"
                     /translation="MSAPPRVSVVIRSFNRHRALCQLLHAVLDQRFTDFEVVVVEQST
                     RPDPEVAAELAALTADPRVRVLHHPPLGGPRARNTGARAARGDILLFMDDDDLPLDGG
                     WIDAHLRNFGDPDCVAATGRWIPEGPVHGPPYRNMEVARARVLSYVPILMFQRVYAQA
                     DRRRRVYSIHGGNVSIRRDALARFGLWDECTSVEDELSLCYRFLRMRGPNEHAVFDPE
                     AVMVRRLDIPGGMDKRYQPLYRLGWRHFEFLHNIIGHYFPVRFVLLYPAYAVLLWVVI
                     LDAVWTDPRRGAARRLAATTWFTLAFPVLWLLWLATLLARRVTNGAPVHHPRLDPMAA
                     PAES"
     gene            complement(1546..2559)
                     /locus_tag="VGB85_23190"
     CDS             complement(1546..2559)
                     /locus_tag="VGB85_23190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0137015.1"
                     /translation="MTTAPALHASIVVRSYNRLPSLCELLAALLAQRCEHSFEIVVIE
                     QSTRVAPADAARLEALAGDPRVRLLRFPPLGGPRARNVGVEAARGDIVALIDDDDLPE
                     DDRWLARLLAAYTDPRCLGISGRQRRWPGDEPTDAYRRAARRKCLGFTPLLRLPTTFV
                     LHDQPVAPVAAVHGTNGSIRRAAFVRFGGWDEDTTVEDESSFAYRAARSMQPGEYFAF
                     DPAPIVVRGLAVAGGLDKRFVSPGRFYRRYLGFIHVIIGRYHPARVVLLYPAYVVAAY
                     AITVAWLWGDSQGHRGAASRTAAALGLLLTLPWHIVVALWNDARAPRRPALPGRDSGA
                     GPR"
BASE COUNT          408 a          885 c         1076 g          335 t
ORIGIN      
        1 acatcggccg cagcagctcg tccgtggtgc cggggtaggt cgtcgattcg agcgagacga
       61 gcatgccggg gtgcagcgcg cgggcgatcg cctggctcgc ggcggtcagc gcccgcaggt
      121 cggggtcccg cgtcttgttg agcggggtcg gcacgcacac gatcgcgacg tcggcggtcg
      181 cgagcgtggc ctcgaagtcg gtcgaggtga acaggcgcgt gccgatcacg cggcgcactc
      241 gctcggccgc gacgtcctcg atgtacgact cgccgcgctg cagcgcgtcg agcttgctgg
      301 cgtcgatgtc gagcccggtg acccgcacaa agccggcctc ggcgaacgcg acagcgagcg
      361 ggaggccgac gtagcccatg cccagcacga cgatatgggc gctgtgatcg gcgagctttt
      421 gcagcaaccg gagatgcgga gcgaccgggt ccatggccgc gagaaagtat ccgaagccct
      481 cgcagggcgg cgaaagacgc gggtgacctg tgtcacgact ccgcgggcgc ggccatcggg
      541 tcgagccgcg ggtgatgcac gggagcgccg ttcgtcacgc ggcgggccag cagggtggcg
      601 agccacagca gccacagcac cgggaaggcg agggtgaacc acgtcgtcgc ggcgagccgt
      661 cgggcggcgc cgcggcgcgg gtcggtccac acggcgtcga ggatcacgac ccacagcagc
      721 acggcgtagg ccgggtagag cagcacgaag cggaccggaa agtagtggcc gatgatgttg
      781 tgcaggaact cgaagtgccg ccagccgagg cggtagagcg gctgatagcg cttgtccatg
      841 ccgccgggga tgtcgaggcg acggaccatc acggcctcgg ggtcgaacac ggcgtgctcg
      901 ttgggcccgc gcatgcgcag gaatcggtag cacagggaca gctcgtcctc gacgctggta
      961 cattcgtccc acagaccgaa acgcgcgagc gcgtctcggc gaatcgagac gttgccgccg
     1021 tggatgctgt acacgcggcg ccggcgatcg gcctgcgcgt agacccgctg gaacatcagg
     1081 atcgggacgt agctgagcac gcgcgcgcgc gcgacctcca tgttgcgata cggcgggccg
     1141 tggaccgggc cctcggggat ccagcggccg gtcgcggcga cgcagtcggg gtcgccgaag
     1201 ttgcgcaggt gggcgtcgat ccacccgccg tcgagcggca ggtcgtcgtc gtccatgaac
     1261 agcaagatgt cgccccgggc cgcgcgtgcg ccggtgttgc gggcccgcgg tccgccgagc
     1321 ggcgggtgat gcaggacgcg cacccgcggg tcggccgtca gcgcggcgag ctcggccgcg
     1381 acctcggggt cggggcgcgt gctctgctcg acgacgacga cctcgaagtc ggtgaagcgc
     1441 tgatcgagga ccgcgtggag cagctggcac agcgcccggt ggcggttgaa cgagcggatg
     1501 acgacggaga cgcggggggg cgcggacatg gcgagcccgc gatctttagc gcgggccggc
     1561 gcccgagtca cgccccggga gcgcgggtcg gcgtggcgcc cgggcgtcgt tccacagcgc
     1621 gacgacgata tgccagggca gggtcagcag caggccgagc gccgcggcgg tccgcgaggc
     1681 ggcgccgcgg tggccctgcg agtcgcccca cagccacgcg acggtgatcg cgtaggcggc
     1741 gacgacgtac gcgggataca gcaggacgac gcgcgccggg tggtagcggc cgatgatcac
     1801 gtggatgaag ccgaggtagc ggcgatagaa gcggccgggc gagacgaagc gcttgtcgag
     1861 gccgccggcg accgcgagcc cgcggacgac gatcggcgcc gggtcgaacg cgaagtactc
     1921 gccgggctgc atcgatcgcg cggcccggta ggcgaaggag ctctcgtcct cgacggtggt
     1981 gtcctcgtcc cagccgccga agcgcacgaa ggccgcgcgg cggatcgacc cgttggtgcc
     2041 gtggacggcc gcgaccgggg cgaccggctg gtcatgcagg acaaaggtcg tcggcaggcg
     2101 cagcagcggg gtgaagccga ggcacttgcg acgcgcggcc cggcgatagg cgtcggtcgg
     2161 ctcatcgccg ggccagcgac gctggcggcc gctgatcccg aggcagcgcg ggtcggtgta
     2221 ggcggcgagc aggcgcgcga gccagcggtc gtcctcgggc aggtcgtcgt cgtcgatcag
     2281 cgcgacgatg tcgccgcggg ccgcctcgac gccgacattg cgggcgcgcg ggcccccgag
     2341 cggcgggaac cgcagcagcc gcacgcgcgg gtccccggcg agcgcctcga ggcgggcggc
     2401 gtccgcgggc gcgacccggg tgctttgttc aatgacgacg atctcgaagc tgtgctcgca
     2461 gcgctgggcc agcagggcgg ccagcagctc gcacagcgag ggcagccggt tgtacgagcg
     2521 gacgacgatc gacgcgtgaa gggcaggggc ggtcgtcatc gtccggccgg ggaccatacc
     2581 actgcgcctt cgtgcaggct cctgcgggct cgctcgcgcg gcggccgcat gctatgcagc
     2641 cggcgcatga gcgtctcgtt ctccgacctc gccgaccggg tgccgtcctc gaccgtgttc
     2701 gtgg
//
LOCUS       DASWNJ010001393         7056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_556328, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001393 DASWNJ010000000
VERSION     DASWNJ010001393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7056
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556328"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..464
                     /locus_tag="VGB85_23195"
     CDS             90..464
                     /locus_tag="VGB85_23195"
                     /inference="COORDINATES: protein motif:HMM:NF015867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEY0137016.1"
                     /translation="MFKSIITLLTCAAAAATLTLSTPAQATPLASAPVYSLLAPELEG
                     QVNLNTATAEQLELLPGIGPSMAKKIIDYRTAKPFAEPMQVVRIKGIGRKTFEKLKPL
                     ISVKGENTLREVSKDETEMKKK"
     gene            762..1709
                     /locus_tag="VGB85_23200"
     CDS             762..1709
                     /locus_tag="VGB85_23200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137017.1"
                     /translation="MRIGRTLSCLVWLALAGAAPACHPKAVEAPYAVGQAPTSEALLA
                     AATPRISALQVSSAKVRIGRSPAANLMFVAQKPSRFSGQVQIAGRELVSLAFHEQGYA
                     LRNVAAEGLPAGFYSGPPADCAIRQLIGVPFSASELIDLVLGGSPRIAGPVEVVEQRW
                     DRRTGHEVLRLRTPSAEQELRFAWVSGAWWVAGSTLWTRNGKGELTRLWSLLHDELRP
                     VEATVLPASTRISRPDGRKDQKVSISYRAQIPNPDLGDASAAAEAEDEGWEDEDTAAS
                     DEGDGDGEAPAPAAAPAKIPAQFMLDGAGLDPRGDLCRR"
     gene            complement(1759..2460)
                     /locus_tag="VGB85_23205"
     CDS             complement(1759..2460)
                     /locus_tag="VGB85_23205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0137018.1"
                     /translation="MAGTILLVDDEQDLIETLRYSLEREGFATLSARSGAAALGILGQ
                     SQIPDLVLLDWMLPDMSGTEVCRTLRMTERTRQVPVFMVSARSEEIDRVVGFELGADD
                     YVTKPFSVRELLLRIRAILRRRASESSSVEEPRFDRLRVDLDRHQVWIDDQPITLTAL
                     EFRLLTTLMARRGRVQTREMLLQDVWGLQIDVTTRTVDTHVQRLRKKLGAVVGNYIET
                     LRGVGYRFLVSDAPS"
     gene            complement(2476..3816)
                     /locus_tag="VGB85_23210"
     CDS             complement(2476..3816)
                     /locus_tag="VGB85_23210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137019.1"
                     /translation="MQRFSIFLACSTLSMLLACDGGGGETAEDGVTTDPTAGGEEVCD
                     SEISGTIEADTRWTCDHTLSDIVTVKNGAKLTIDPGVTIKGSNGSALVIGRGSQLFAE
                     GTADKPIVFTSALPAGSRARGDWGGLVLLGDAPNNLQTGTGAAEGLDANEPSYQYGGT
                     DAASSCGSLKYVRVEFAGFELTKDNELNGITFYSCGSGTQVDYVQSHMGKDDGIEAFG
                     GTWSGTHIVITGALDDAFDADQGYVGNLQYVFIAQDTATGNFGFEWSNQKDNLDALPR
                     THPVVSNVTFIGTGTNDGSFVETKSSGIKLKEGSGGEIHNSIIAYSYNAAIELTEEAT
                     EVVADAGDIKITDTILFKNARKDDGASIYAGSEGSTWDLAGFVEAAGNNNHIEVDPML
                     GSSAWGAADIVPMAGSPVIGAGRPVPGHEATDFIGAVQPGAGDWTRGWTSYALN"
     gene            complement(3892..7035)
                     /locus_tag="VGB85_23215"
     CDS             complement(3892..7035)
                     /locus_tag="VGB85_23215"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY0137020.1"
                     /translation="MPLLDRKSPTLLAALATFGIGVSETQAAGPRDYEGPQGWSVDGE
                     AAPPPDATPPADTPPDADFPVEDAAPAEPAPPAAPPPEDDSGCADGDFCVEDLTEDAD
                     ALAKEMAPKATPKPVGPSGTISGRVLDAVSGSPLIGVNITVPGTDFKTKTDVDGRYVM
                     PVPPGTYQVRVWYDAYEGMTISGVVVATDALININRELRPIAGMTQTVAVTAEINKES
                     SAGKLVERKKSVAARDMMSRDDIRKSGGGATSAVARRIVGSTIVGDRFLFVRGLGHRY
                     GNTLFDGARLPSPDPNLRTVPLDIFPSGALSAINVQKTATPDVPADFAGASVQLESRE
                     TPEKWSFTLDARFGINTATSFKPGLRGDHFKGDNFTTGNLGRDLPGLYDTRVPIGPTT
                     TIAGKQAFTPAQLEKFGESLPSLNTAIGRYTALPNFAGGLQLGNTFKPWGTRLGFLAA
                     ANYANTTQTLQETHRIYNTTRPGGDGPYELNTRNPDVELEGTKTTQNVQMSALGLIKW
                     KLDEHNRIALTTLYTRDADNESRVLEGRLRTTCGEQFYCRNTRLRYIMRSVLFTRLGG
                     KHEIPAAKGLTVEWFGSYAQARQDDPLLREMLFNRASDSGADDSNYNVDSNESGKFQF
                     FKLLDHAGSGALDFTVPFKQWGQLDSRFKFGAWAEAKRRTFGVRTFDFEPRGWQGALP
                     TGTGNIINADTVGGGEPGRASEPFVINDVTRPNGQDGYAARQEVYAAYGMLDLPLVRW
                     ARLVGGARFEANNIRVAPFDTFGQTVAKTSATIRTNDVLPSVSLIFAARKDMNVRLVG
                     AETVARPEFRELAPFVFTDFAGGFTVQGNNKLQATKIWNADLRWEWFPSANEVLAVSV
                     FYKRFDAPIERIIITNPRLQTFTNARFANNVGAELEARKNLEFMHKSMRDFSLGFNFA
                     YIFSRVQIEAPKEGDVLAPTSTQRYLEGQSPYVLNAFLGYDNERSGTNARVLFNTFGR
                     RIAFVGGNHLPDVYELPVHTLDVTLSQKVHKNLTLTFSALNMLNWRRRLVQGPSDEIF
                     YSARRGVFFIFGLSYSI"
BASE COUNT         1035 a         2315 c         2530 g         1176 t
ORIGIN      
        1 cgtacatcgt tgaaattaca tgaactgcgc gcaggcgccg ggcttgcttt ggtggtcccg
       61 tcggcatcac gccgcggaga accacgatca tgttcaagtc catcatcacg ctgctcacct
      121 gcgccgccgc tgcggccacc ctcaccctct ccacccccgc ccaggcgacg ccgctggcga
      181 gcgcgccggt ctactcgctg ctcgcgccgg agctcgaggg tcaggtcaac ctcaacaccg
      241 cgaccgcgga gcagctcgag ctgctgccgg ggatcgggcc gtcgatggcc aagaagatca
      301 tcgactaccg caccgccaag cccttcgccg agccgatgca ggtcgtccgc atcaagggca
      361 tcggccgcaa gaccttcgag aagctcaagc ccctgatctc cgtcaagggc gagaacaccc
      421 tgcgcgaggt cagcaaggac gagacggaga tgaagaagaa gtagctgtcc cggggcgggc
      481 agcagccccg cccgccaggg caacgggcac gcgccacgaa cggcgcgtgc gcccgggaca
      541 gtgacgagcg cggcgggccc tggtcccgtc cgtcgcgccc gtccctggac ccgggtaggc
      601 cacgcatggc atcgaggccg atgtgcatgg ccggagggcc cgcgtgtggt caggggcgac
      661 tcgtcgtgcg tgcacggcga ccgcgtccgg ccacgcgcgg gcgtttttct ggcgccggct
      721 cgcagtgacg ggccgcgaga ttagggctag ggttcgcgga catgcggatc ggtcgcaccc
      781 tctcttgcct ggtctggctg gcgctcgccg gcgccgcgcc tgcctgccat ccgaaggccg
      841 tcgaggcgcc ctacgccgtc ggccaggcgc cgacctccga ggccctgctc gccgccgcga
      901 ccccgcggat ctcggcgctg caggtgtcgt cggccaaggt gaggatcggc cgctcacccg
      961 cggccaacct gatgttcgtc gcccagaagc cgtcgaggtt ctcgggccag gtgcagatcg
     1021 ccggccgcga gctggtgtcg ctggcgtttc acgagcaggg ctacgcgctg cgcaacgtcg
     1081 ccgccgaggg cctgcccgcc ggattctaca gcggaccgcc cgccgactgc gcgatccggc
     1141 aattgatcgg ggtgccgttc tcggcgagcg agctgatcga cctcgtgctc ggcggctcac
     1201 cgcgcatcgc cgggccggtc gaggtggtcg agcagcgctg ggaccgacgc accggccacg
     1261 aggtcctgcg cctgcgcacc ccgagcgcgg agcaggagct gcgcttcgcg tgggtctccg
     1321 gcgcgtggtg ggtcgccggc agcacgctgt ggacaaggaa cggcaagggc gagctgacgc
     1381 gcctgtggtc gctgttgcat gacgagctgc ggccggtcga ggcgacggtg ctgcccgcga
     1441 gcacgcggat ctcccggccc gacggacgca aggatcagaa ggtgtcgatc agctaccgcg
     1501 cgcagatccc caacccggac ctcggcgacg cgagcgcggc cgccgaggcg gaggacgagg
     1561 gctgggagga cgaggacacc gccgcgagcg acgagggcga cggcgatggc gaggcccctg
     1621 cccccgcggc cgcaccggcg aagatccccg cgcagttcat gctcgacggc gccggcctcg
     1681 acccgcgcgg cgacctctgc cggcgatgat caagcggatc aagcggatca agcggatcaa
     1741 gcggatcaaa gctgatcctc agctcggcgc gtcggacacg aggaagcggt aaccgacgcc
     1801 gcgcagcgtt tctatataat tgccgaccac cgcgccgagc ttcttgcgca gccgctgcac
     1861 gtgggtgtcg acggtgcgcg tggtgacgtc gatctgcagg ccccagacat cctgtaacaa
     1921 catctcgcgg gtctgcacgc gcccgcgacg ggccatcagg gtggtcagca ggcggaactc
     1981 gagggcggtg agcgtgatcg gctggtcgtc gatccacacc tgatgccggt cgaggtcgac
     2041 gcgcaggcga tcgaagcgcg gctcctcgac gctggaggat tcgctggcgc ggcggcggag
     2101 gatcgcccgg atccgcagca gcagctcgcg cacgctaaac ggcttggtca cgtagtcgtc
     2161 ggccccgagc tcgaagccga cgacgcgatc tatctcctcg ctgcgcgcgc tgaccatgaa
     2221 cacggggacc tggcgggtcc gctcggtcat ccgcagcgtg cggcacacct cggtgcccga
     2281 catgtcgggc agcatccagt cgagcagcac gaggtcgggg atctggctct gcccgaggat
     2341 ccccagcgcc gccgcgccgc tgcgcgccga gagcgtggcg aagccctccc gctcgaggct
     2401 gtagcgcagc gtctcgatca ggtcttgctc gtcgtcgacc aggaggatcg tgcctgccat
     2461 gatgatgcgt gcccctcagt tcagcgcgta gctggtccag ccgcgggtcc agtcgccggc
     2521 gcccggttgc acggcaccga tgaagtcggt cgcctcgtgg cccggcaccg gacgaccggc
     2581 gccgatcacc ggcgagccgg ccatcggcac gatgtcggcg gctccccagg cgctgctgcc
     2641 gagcatcggg tcgacctcga tgtggttgtt gttgccggcc gcctcgacga agccggcgag
     2701 gtcccaggtc gagccctcgc tgccggcgta gatgctggcg ccgtcgtcct tgcgggcgtt
     2761 cttgaacagg atggtgtcgg tgatcttgat gtcaccggcg tcggcgacga cctcggtcgc
     2821 ctcctcggtc agctcgatcg cggcgttgta cgagtacgcg atgatcgagt tgtggatctc
     2881 gccgccgctg ccctccttga gcttgatgcc gctgctcttg gtctcgacga agctgccgtc
     2941 gttggtgccg gtgccgatga aggtcacgtt cgacacgacc gggtgcgtgc gcggcagggc
     3001 gtcgaggttg tccttttggt tcgaccactc gaagccgaag ttgccggtcg cggtgtcctg
     3061 cgcgatgaac acgtactgca ggttgccgac gtagccctgg tcggcgtcga aggcgtcgtc
     3121 cagcgcgccg gtgatcacga tgtgcgtgcc gctccaggtg ccgccgaacg cctcgatgcc
     3181 gtcatccttg cccatgtgcg actgcacgta gtcgacctgc gtgcccgagc cgcacgagta
     3241 gaaggtgatg ccgttgagct cgttgtcctt ggtcagctcg aagccggcga actcgacgcg
     3301 cacgtacttg agcgagccgc agctcgacgc cgcgtcggtg ccgccgtact gatacgaggg
     3361 ctcgttggcg tcgaggccct cggccgcgcc ggtgccggtc tggaggttgt tgggcgcgtc
     3421 gcccagcagc acgaggccgc cccagtcgcc gcgcgcacgc gagccggcgg gcagcgccga
     3481 ggtgaacacg atcggcttgt cggcggtgcc ctcggcgaac agctgcgagc cgcgcccgat
     3541 caccagcgcg ctgccgttgc tgcccttgat cgtgacgccg gggtcgatcg tcagcttcgc
     3601 gccgttcttg acggtgacga tgtccgagag cgtgtgatca caggtccagc gggtgtcggc
     3661 ctcgatcgtg ccgctgatct cgctgtcaca gacctcctcg ccgcccgcgg tcgggtcggt
     3721 ggtcacgccg tcctctgcgg tctctccgcc gccgccgtcg caggcgagga gcatcgagag
     3781 ggtcgaacag gccaggaaga tggagaaacg ctgcatggcg gatgtcatat cggcgggagg
     3841 tgacgcaagt tcggcggcgt cgtgacaagg ttgcgacagg acgtgaacgt ttcagatgct
     3901 gtagctcagg ccgaagatga agaagacgcc gcggcgggcc gagtagaaga tctcgtcgct
     3961 cgggccctgc accaggcggc ggcgccagtt gagcatgttg agcgcgctaa acgtcagcgt
     4021 caggttcttg tggaccttct gcgagagcgt gacgtccagc gtgtggaccg gcagctcgta
     4081 gacatcgggc agatgattgc cgccgacgaa cgcgatccgg cggccgaagg tgttgaacag
     4141 gacgcgcgcg ttggtgccgc tacgctcgtt gtcgtagccc aggaaggcgt tgaggacata
     4201 cggcgactgg ccctcgaggt agcgctgcgt cgaggtcggc gcgagcacgt cgccctcctt
     4261 cggcgcttcg atctgcaccc gcgagaagat gtaggcgaag ttgaagccga gcgagaagtc
     4321 gcgcatcgac ttgtgcatga actcgaggtt cttgcgcgcc tcgagctcgg cgccgacgtt
     4381 gttggcgaag cgggcgttgg taaaggtctg gagccgcggg ttggtgatga tgatgcgctc
     4441 gatcggcgcg tcgaagcgct tgtagaacac gctgaccgcg agcacctcgt tggcgctcgg
     4501 gaaccactcc cagcgcaggt cggcgttcca gatcttggtc gcctggagct tgttgttgcc
     4561 ctgcacggtg aagccgccgg cgaagtcggt gaacacgaag ggcgcgagct cgcgaaactc
     4621 cggccgcgcg accgtctcgg cgccgaccag gcgcacgttc atgtccttgc gcgcggcgaa
     4681 gatcaggctg accgagggca ggacgtcgtt ggtgcggatc gtcgcgctcg tcttggccac
     4741 cgtctgcccg aacgtgtcga agggcgcgac gcggatgttg ttggcctcga agcgcgcacc
     4801 accgaccagc cgcgcccagc ggaccagcgg caggtcgagc atcccgtagg ccgcgtacac
     4861 ctcctggcgc gcggcgtagc cgtcctgacc gttgggccgg gtgacgtcgt tgatgacgaa
     4921 gggctcgctc gcgcggcccg gctcgccgcc gccgacggtg tcggcgttga tgatgttgcc
     4981 ggtgccggtc ggcagcgcac cctgccagcc gcgcggctcg aagtcgaagg tgcgcacgcc
     5041 gaaggtgcgg cgcttggcct ccgcccacgc gccgaacttg aagcgcgagt cgagctggcc
     5101 ccactgtttg aagggcaccg tgaagtcgag cgcgccgctg ccggcgtggt cgagcagctt
     5161 gaaaaattgg aacttgccgg actcgttgga gtcgacgttg tagttgctgt cgtccgcgcc
     5221 ggagtcgctg gcgcggttga acagcatctc gcgcagcagc gggtcgtcct ggcgcgcctg
     5281 cgcgtaggac ccgaaccact cgaccgtgag gcccttcgcc gcggggatct cgtgcttgcc
     5341 gccgaggcgg gtgaacagca ccgagcgcat gatgtaacgc aggcgggtgt tgcggcagta
     5401 gaactgctcg ccgcaggtgg tgcgcagccg gccctcgagc acgcgcgact cgttgtcggc
     5461 gtcgcgggtg tacagggtcg tcaaggcgat gcggttgtgc tcgtcgagct tccacttgat
     5521 caggccgagc gccgacatct ggacgttctg cgtcgtcttg gtgccctcga gctcgacgtc
     5581 ggggttgcgc gtgttgagct cgtaggggcc gtcaccgccc ggccgggtgg tgttgtagat
     5641 cctgtgcgtc tcttgcaggg tctgcgtggt gttggcgtag ttcgccgcgg ccaggaagcc
     5701 gaggcgcgtg ccccagggtt taaaagtgtt gccgagctgc aggccgcccg cgaagttcgg
     5761 cagcgcggtg tagcggccga tcgcggtgtt gagcgagggc aaggactcgc cgaatttttc
     5821 taactgcgcc ggcgtgaacg cctgcttgcc ggcgatcgtc gtcgtcgggc cgatcgggac
     5881 cctggtgtcg tagaggccgg gcaggtcgcg gccgaggttg cccgtcgtga agttgtcgcc
     5941 tttaaaatga tcgccgcgca ggcccggctt gaagctcgtc gcggtgttga tgccgaagcg
     6001 ggcgtcgagc gtgaacgacc acttctccgg ggtctcgcgg ctctcgagct gcaccgaggc
     6061 gcccgcgaag tcggccggca cgtctggcgt cgccgtcttc tgcacgttga tcgccgacag
     6121 cgcgcccgac gggaagatgt cgagcgggac ggtgcgcagg ttgggatcgg ggctcggcag
     6181 gcgcgccccg tcgaacaggg tgttgccgta gcggtgcccg aggccgcgca cgaacaggaa
     6241 gcggtcgccg acgatcgtgc tgccgacgat gcgccgcgcc accgccgagg tcgcgccgcc
     6301 gccggacttg cggatgtcgt cgcggctcat catgtcgcgc gcggcgaccg acttcttgcg
     6361 ctcgaccagc ttgccggccg agctctcctt gttgatctcg gcggtgacgg ccaccgtctg
     6421 cgtcatgcca gcgatcggcc gcagctcgcg gttgatgttt attaaagcgt cggtcgcgac
     6481 gacgacgccg gagatcgtca tgccctcgta ggcgtcgtac cagacgcgga cctgatacgt
     6541 gcccggcggc accggcatca cgtagcgtcc gtcgacgtcg gtcttcgttt taaaatcggt
     6601 gccggggacg gtgatgttga cgccgatcag cggtgaacca ctgaccgcat cgagcacgcg
     6661 cccggagatc gtgccactcg ggcccacggg cttcggcgtg gccttcgggg ccatctcttt
     6721 tgcaagagcg tcggcgtcct cggtcaggtc ctcgacgcag aagtcaccgt cggcgcaccc
     6781 gctgtcgtct tccggcggag gcgcggctgg cggagcgggc tcggcgggcg ccgcatcctc
     6841 gaccgggaag tcggcgtcgg gtggcgtgtc tgcgggcggc gtcgcgtccg ggggcggggc
     6901 ggcctcaccg tcgacgctcc agccctgcgg accttcatag tcgcgcgggc cagcggcctg
     6961 ggtttcggat acaccgatcc cgaacgtcgc cagcgcggcc agcaacgtcg ggctcttgcg
     7021 gtcgagcaag ggcatggttt ttctgaattt tcagcg
//
LOCUS       DASWNJ010001394         3532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_129561, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001394 DASWNJ010000000
VERSION     DASWNJ010001394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129561"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1454
                     /locus_tag="VGB85_23220"
     CDS             <1..1454
                     /locus_tag="VGB85_23220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0137021.1"
                     /translation="ETIRQVRMGVMFFTGMTAVIIGSLQLLSAGESPGSWDRVNGLAY
                     IVTAAGCFAALALTRTSLLRVSEITALMTAYFGTASVIVTGSGEDSALIVGVASYSLV
                     VLLPGIAFRTRSARWSAYNVAGVCVVYVASVFLRLALHNNGPWNLPVGELVIKLCTPP
                     MAFWLQWLMVRALNNRILEALQESERSRSALAVSNQLLELANRSLETARIEAERIRDA
                     AEAANRAKSMFLANMSHELRTPLNSIIGYTELIMDEARDDEEARVRAVVPDLKNVVLS
                     AKHLLGLIGGILDLSKIEAGRMELVQEQFSVLDFVREVEQTIAPQVRKRGNALIVRCS
                     EDTGIVVADRGKLKQVLLNLLGNAVKFTNDGEITLASHRDREQSLLTFEVRDTGIGIE
                     PDKLAVIFDKFTQIDASPTRRYEGAGLGLAITRELCAMMGATIDVQSRPNNGTVFTVV
                     LPIHIPLASQSLTLSSQSSTSGAAASTSSSVLR"
     gene            1580..2794
                     /locus_tag="VGB85_23225"
     CDS             1580..2794
                     /locus_tag="VGB85_23225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0137022.1"
                     /translation="MTHEVDLLIIGSGPAGCSTALHLARLDPALARRTVVLDRNEHPR
                     HKLCGGGLVSDVDTILDNLGLDIDEVPHVDAGWAQLHFQGRGIRMKLKDIAFHVVRRR
                     EFDSWLAAKVRDSGVALYEKTQALSLRQVDGGVLVETNRGSFRARAVVGADGTKGFVR
                     RAISGDAGPIARVIEVMVPPPRGPSPEPIVADDEALFEFRNIASGVQGYFWSFPMQIE
                     GKSMRNLGVYDSRAIDAPLAGSLKGFLEEGLARQGVRLEDCKVEGHPIHLFAPRSVLS
                     APNIILVGDAAGVDPLLGEGISLALGYGELAARAIVDGFTRDDLSFRDYTRRIHRSPM
                     GHSMRYRYHAARLIYRMHHPALQRIVWWYLQPVVRRFILDFVFNWARTSNLPPAPPRP
                     SMHDAGRLAAAR"
     gene            2816..>3532
                     /locus_tag="VGB85_23230"
     CDS             2816..>3532
                     /locus_tag="VGB85_23230"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY0137023.1"
                     /translation="MQPDAFYHPDGDLLVPTALTRGPWHIDHQHGGPPAALLARAIEG
                     LAPPALWHPARFTLDFLRPVPVTRPLRAVAELTRRGKQVLGLTARLLDGDQPVASASA
                     LCIRRLEHDLDLPAGAAPAEQRRDPESFPAFDFSFFRWDIGYHTAVDGRLEAGEIGRG
                     PATAWMRTRQPLVAGEPTSALQHVLIVADAINGVGFVLDLADHTFVNADLGVHLHRLP
                     VDAWIRMSAIPSPQPHGVGLV"
BASE COUNT          502 a         1389 c         1093 g          548 t
ORIGIN      
        1 acgagacgat ccgtcaggtt cgcatgggcg tcatgttctt caccggcatg accgcggtga
       61 tcatcggatc gctgcagctg ctgtccgccg gtgagagccc cggctcgtgg gaccgcgtca
      121 acggcctcgc gtacatcgtc accgccgccg gctgcttcgc cgccctcgcc ctgacgcgca
      181 cctcgctgct gcgcgtctcg gagatcacgg cgctgatgac cgcctacttc ggcaccgcgt
      241 ccgtcatcgt caccggctcg ggcgaggact cggcgctgat cgtcggcgtc gcctcgtaca
      301 gcctcgtcgt cctcctgcct ggcatcgcct ttcgcacccg ctcggcccgc tggagcgcct
      361 acaacgtcgc cggcgtgtgc gtcgtctacg tcgccagcgt gttcctccgc ctcgccctgc
      421 acaacaacgg cccgtggaac ctgccggtcg gcgagctcgt gatcaagctg tgcaccccgc
      481 cgatggcctt ctggctgcag tggctgatgg tgcgcgcgct caacaaccgc atcctcgagg
      541 ccctgcagga gagcgagcgc tcgcgcagcg cgctggccgt ctcgaaccag ctgctagagc
      601 tcgccaaccg cagcctcgag acggcgcgca tcgaggccga acggatccgc gacgccgccg
      661 aggcggccaa ccgggccaag agcatgttcc tggccaacat gagccacgag ctgcgcacgc
      721 cgctcaactc gatcatcggt tacaccgagc tgatcatgga cgaggcgcgc gacgacgagg
      781 aggcccgcgt ccgcgcggtc gtgccggacc tcaagaacgt cgtgctctcg gccaagcacc
      841 tgctcggcct gatcggcggc atcctcgacc tctcgaagat cgaggccggc cgcatggagc
      901 tcgtgcagga gcagtttagc gtcctcgact tcgtccgcga ggtcgagcag acgatcgccc
      961 cgcaggtgcg caagcggggc aacgccctga tcgtgcgctg cagcgaggac accgggatcg
     1021 tcgtcgccga ccgcggcaag ctcaagcagg tgctgctcaa cctgctcggc aacgccgtca
     1081 agttcaccaa cgacggcgag atcacgctcg cctcgcaccg cgaccgcgag cagtcgctgc
     1141 tgaccttcga ggtccgcgac accggcatcg gcatcgagcc cgacaagctc gccgtcatct
     1201 tcgacaagtt cacgcagatc gacgcctcgc cgacccgccg ctacgagggc gccggcctcg
     1261 gcctcgcgat cacgcgggag ctgtgcgcca tgatgggcgc cacgatcgac gtccagagcc
     1321 gtcccaacaa tgggaccgtc ttcacggtcg tcctcccgat ccacattccg ctggcgagcc
     1381 agtcgctgac cctgtcgagc cagtcctcga cctccggcgc cgccgcctcg acctcgagca
     1441 gcgtccttcg ctgatcgcct ttcgtgggcc ctgcgccgct gtttggcgcc aggaccatgt
     1501 tcaggtgagc ccgcgcacga cgcgccttta cgagcgtatg ctcgacaacg cgctatcgtg
     1561 agtcgcgcga ggcgcgatca tgacacacga ggtcgaccta ctcatcatcg gctccggccc
     1621 cgcgggctgc tccacggcgc tccatctcgc gcggctcgat cccgcactgg cccgcaggac
     1681 cgtcgtcctc gaccgcaacg agcacccccg gcacaagctg tgcggcggcg gcctcgtcag
     1741 cgacgtcgac acgatcctcg acaacctcgg cctcgacatc gacgaggtcc cgcacgtcga
     1801 cgccggctgg gcccagctgc acttccaggg tcgtgggatc cggatgaagc tcaaggacat
     1861 cgcgttccac gtcgtgcgtc gccgcgagtt cgacagctgg ctcgccgcca aggtccgcga
     1921 cagcggcgtc gccctgtacg agaagaccca ggcgctgagc ctgcgtcagg tcgacggcgg
     1981 cgtgctcgtc gagaccaacc gcggcagctt ccgggcccgc gcggtcgtcg gggccgacgg
     2041 caccaagggc ttcgtgcggc gtgcgatctc cggcgacgcc ggcccgatcg cccgcgtcat
     2101 cgaggtgatg gtcccgccgc ctcgcggccc ctcgcccgag ccgatcgtcg ccgacgacga
     2161 ggccctgttc gagtttcgca acatcgcctc cggcgtgcag ggctacttct ggagcttccc
     2221 gatgcagatc gagggcaagt cgatgcgcaa cctcggcgtc tatgacagcc gggcgatcga
     2281 cgccccgctc gcgggctcgc tcaagggctt cctcgaggag ggcctcgcgc gccagggtgt
     2341 ccgcctcgag gactgcaagg tcgagggcca cccgatccac ctgttcgccc cgcgcagcgt
     2401 gctgtcggcg cccaacatca tcctcgtcgg cgacgcggcc ggcgtcgatc cgctgctcgg
     2461 cgagggcatc agcctcgcgc tcggctacgg cgagctggcc gcgcgcgcga tcgtcgacgg
     2521 cttcacgcgc gacgacctga gctttcgcga ctacacccgc cggatccatc gcagcccgat
     2581 gggccactcg atgcgctacc gctaccacgc cgcccgcctg atctatcgca tgcaccaccc
     2641 cgccttgcag cggatcgtct ggtggtacct gcagccggtc gtgcgccgct tcatcctcga
     2701 cttcgtgttc aactgggccc gcaccagcaa cctgccgccc gcgcccccgc gcccgagcat
     2761 gcacgacgcc ggcagactcg ccgccgcccg ctgaggtacg ctcctcgcgt ccgcgatgca
     2821 gcccgacgcc ttctatcacc ccgacggcga cctcctcgtc ccgaccgcgc tcacccgcgg
     2881 tccctggcac atcgaccacc agcacggcgg cccaccggcg gccctgctcg cgcgcgcgat
     2941 cgaggggctc gccccgcccg cgctgtggca cccggcgcgc ttcaccctcg acttcctgcg
     3001 ccccgtcccc gtcacccggc cgctgcgcgc tgtcgccgag ctgactcgtc gtggcaagca
     3061 ggtgctcggc ctgaccgccc gcctgctcga cggtgaccag ccggtcgcct ccgccagcgc
     3121 gctgtgcatc cgtcgcctgg agcacgacct cgacctcccg gccggcgctg ccccggccga
     3181 gcagcgccgc gaccccgaga gcttcccggc cttcgacttc tccttctttc gctgggacat
     3241 cggctatcac accgcggtcg acggccgcct cgaggccggc gagatcggcc gcggccccgc
     3301 gaccgcgtgg atgcgcaccc gccagccgct cgtcgccggt gagccgacct cggccctgca
     3361 gcacgtgctc atcgtcgccg acgcgatcaa cggcgtcggc ttcgtcctcg acctcgccga
     3421 tcacactttt gtaaatgccg acctcggcgt ccacctgcat cgcctgcccg tcgacgcctg
     3481 gatccggatg tccgcgatcc cctcgccgca accacacggc gtcgggctcg tc
//
LOCUS       DASWNJ010001395         2029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_556338, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001395 DASWNJ010000000
VERSION     DASWNJ010001395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556338"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2029
                     /locus_tag="VGB85_23235"
     CDS             <1..>2029
                     /locus_tag="VGB85_23235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0137024.1"
                     /translation="APAPARSGKAGDLSEPLAAALPAVSRLLRRALAGEPVDDITLLE
                     ADTVSLQDVAVLAVPALVATLADEATVDRFLAMLARGDLLSPAAAADALASNGLLALA
                     VPALARFAPERFSSAGLLVRLGKTREHAGDDDETSAAALAFAERALVLAPGDPDALAL
                     AAGLNRRLRRHGAALPLFRELHERQPGDLGVLERLAALERDALAREPTRGRADLVLWS
                     TSLREARLRALLAAPGDSVARYEFARALILAEEFDAARAVLESLLADDPGHLRAARDL
                     LRVAQLGGEPAAVMVAVPRLLALERSDDAVVALAKALRQEERFDEAGALLSRHAADTD
                     NPRIAREQVRNLFFLARFAEAAALGEACVDRHPEDLELRLLTAAARLELGQWREAERH
                     VCYVAASGGDERLASETALYLFATTRKAGLLGPAFARLDPLFLAMGCQTVRPGGPAER
                     PVFDRLEGRGRYPASGSADGFKPVLDGPLVSVVMTAHDAAAYVETAVRSIRDRSYRTL
                     DLVVVDDASTDATPDILEMLERRDPRLRAIVKTGNDGTYVAKNLGLLQARGAYVALQD
                     ADDWSHPDRLAKSVAVLEALPDVVGLTTDWLRMTSDGEVVVKAGGQIAHVCCISLVFR
                     REPVLRRVGFFDSVRIEADMEYIRRMGLAFGPGAVLRLRWPLLFGRSRSDSL"
BASE COUNT          233 a          774 c          726 g          296 t
ORIGIN      
        1 ggcacccgcc ccggcgcgct cgggaaaggc gggagacctc tccgaacccc tcgccgcggc
       61 gttacccgcg gtctcccgcc tcctgcggcg cgcgctcgcc ggggagccgg tcgacgatat
      121 cacgctcctc gaagccgata ccgtctcgct ccaggatgtc gctgtcctgg ccgtgcctgc
      181 gctggtcgcg acgctcgcgg acgaggccac cgttgaccgc ttcttagcca tgctcgcgcg
      241 cggtgacctc ctttccccgg ccgcggccgc agacgccttg gcgtccaatg gcctgcttgc
      301 gctcgccgtg cccgcgctcg cccgattcgc cccggagcgg ttctcctcgg ccgggctgct
      361 cgtccgtctc ggcaagacgc gtgagcacgc cggcgacgac gacgagacct cggcggcagc
      421 gctcgccttc gccgagcgcg cgctggtgct ggccccgggc gacccggacg cgctggcgct
      481 agccgcgggc ctgaaccgcc ggctgcgccg gcacggtgcg gcgcttcccc tgttccgcga
      541 gctgcacgag cggcagccgg gtgacctcgg cgtcctcgag cgtctggcgg cgctggagcg
      601 cgacgccctc gcgcgcgagc cgaccagggg tcgcgccgac ctagtgcttt ggtcgacgtc
      661 gctgcgggag gcccgcctgc gggcgctcct ggcggccccc ggcgactcgg tcgcgcggta
      721 cgagttcgcg cgtgcgctga tcctggccga ggagttcgac gcggcccgcg cggttctcga
      781 gagcctgctg gccgacgacc ccggccacct ccgcgccgcg cgcgacctcc tgcgggtggc
      841 ccagctcggg ggcgagcccg ccgccgtcat ggtcgccgtg ccgcgcctac tggcgctcga
      901 gcgctcggac gacgccgtcg tcgccctcgc gaaggcgttg cgccaggagg agcgtttcga
      961 cgaggcggga gctctcctgt cccgacacgc cgccgacacg gacaacccgc ggatcgcgcg
     1021 cgagcaggtg cgcaacctgt tcttcctcgc ccgcttcgcc gaggccgcgg cgctcggcga
     1081 ggcctgtgtc gaccggcatc ccgaggacct ggagctgagg ctcctgaccg ctgccgcccg
     1141 gctcgaactc ggccagtggc gcgaggccga gcgccacgtc tgctacgttg ccgccagcgg
     1201 cggggacgag cggctggcgt cggagaccgc gctctacctg ttcgcgacga cacgcaaggc
     1261 tggcctgcta ggccccgcct tcgcgcggct cgacccgctg ttcctggcca tgggctgcca
     1321 gacggtccgc ccaggcggac cggcggaacg tccggtcttc gaccggctcg aaggtcgggg
     1381 gcgctacccg gcatccggtt cggcggacgg gttcaagcct gtcctggacg ggccgctggt
     1441 ctcggtggtc atgaccgccc acgacgcggc ggcctacgtc gagaccgcgg tccgctcgat
     1501 ccgcgaccgg agctaccgca ccctggacct cgtcgtggtc gacgacgcca gcaccgacgc
     1561 cacgccggac atcctggaga tgctggaacg gcgcgacccg cggctgcggg cgatcgtcaa
     1621 gacgggcaac gacggcacct acgtggccaa gaacctcggg ctgctccagg cgcgaggggc
     1681 ctacgtggcg ctccaggacg ccgacgactg gtcgcacccg gaccggctcg ccaagagcgt
     1741 ggccgtcttg gaagcgctgc cggacgtcgt cggcctcacc acggactggc tgcggatgac
     1801 ctcggacggc gaggtcgtcg tcaaggccgg cgggcagatc gcccacgtct gctgcatatc
     1861 gctggtgttc cgccgcgagc ccgtgctgcg gcgggtcggc ttcttcgaca gcgtccgcat
     1921 cgaggccgac atggagtaca tccggcggat gggcctcgcg ttcgggccgg gcgccgtgct
     1981 caggctgcgc tggcccctcc tgttcggccg ttcccgctcg gactcgctc
//
LOCUS       DASWNJ010001396         2442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_446873, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001396 DASWNJ010000000
VERSION     DASWNJ010001396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446873"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..1235
                     /locus_tag="VGB85_23240"
     CDS             162..1235
                     /locus_tag="VGB85_23240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137025.1"
                     /translation="MSLLHATAPELEVITDVAPAWVVRGHSLQLEAMRNAAKALRERF
                     ASEPPVVSVRTFDRYTFGYPVRYGFWGMALAASPYTLIRGRTVMVQFRQAGALRTLLF
                     NPSDEQAVKLAPFMVRQMQQKGPLGRMMLDLHGPLEPKLAEAGVAPESVDYVAFDHFH
                     LQDLRGLVSPHEGRPARFPRARLLAQRREWDQWDDLHPLHQAFAVPGAKEGIDPAGVV
                     LHDIDARLGDGVMLVRTPGHTSGNQTLFFKTDTGIWGVSENGVSADNWSPQASRIRGV
                     RGRVGLHGLEVLPNMNTPESTADQYLSMIFERSVVDPARGGDFLQMYPSFEVTHSWLT
                     PGIRAYMHGELRFGEVARPGASA"
     gene            complement(1369..1980)
                     /locus_tag="VGB85_23245"
     CDS             complement(1369..1980)
                     /locus_tag="VGB85_23245"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEY0137026.1"
                     /translation="MKHAPTLLITALAVALGACASKPKQDVAPPTEAPPPEPMAVDAT
                     VAPEPEPMPAEAEPPAPPAATKDVLDTIAEAGNFTTMLSAIEAAGLKDTLKGPGRFTL
                     FAPDDEAFAKIPKPELDRLLKDKKKLANVLNYHIVSGAALTATEIAEMPSVSSVAGPQ
                     ILVSNESGSVKLNGGSSVRSADIKATNGVVHVIDTVLKLPKKK"
     gene            complement(1996..>2442)
                     /locus_tag="VGB85_23250"
     CDS             complement(1996..>2442)
                     /locus_tag="VGB85_23250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137027.1"
                     /translation="EVIGNALRYILRLSQPEAPERTIVLACFADEEHAVPLYGVALRF
                     TEWNFRAVLLGARTPPHAIRHAVAEVHPALVGLSLTVVPPAYRVRELIDGYGEACGKT
                     PWLVGGAGAEKVRDLVEARGGLLVPNERAKLKALVDRLAGPRGDST"
BASE COUNT          329 a          870 c          868 g          375 t
ORIGIN      
        1 gcccacattc cgggcgcgcg cgaccgcccc cgggcgcgcc cgccggtcgc cggccgcgct
       61 cgatcggtcg cccgcgcgtg gcgccgcggg agaacgccgc aggtgcagcc accgatctat
      121 atcggcttgc ccgatcgacg cggccgtcca gaaaaacggc catgagtctg ctccacgcga
      181 cggcacccga gctcgaggtc atcaccgatg tcgcccccgc atgggtcgtg cggggtcatt
      241 ccctgcagct ggaggcgatg cgcaacgccg cgaaggcgct gcgcgagcgc ttcgccagcg
      301 agccgccggt cgtcagcgtg cggaccttcg accgttacac cttcggctat ccggtccgct
      361 acgggttctg gggtatggcg ctggcggcga gcccgtacac gctgatccgc ggccggaccg
      421 tgatggtgca gtttcgccag gccggagcgc tacgcaccct gctgttcaat cccagcgacg
      481 agcaggccgt caagctcgcc ccgttcatgg tccggcagat gcaacaaaag ggcccgctcg
      541 ggcgcatgat gctcgacctg cacgggccgc tggagcccaa gctcgcggag gccggggtcg
      601 cgccggagtc cgtcgactac gtcgcgttcg accacttcca cttgcaggac ctgcgcgggc
      661 tcgtcagccc gcacgagggc cgccccgcgc gattcccccg ggcgcgcctg ctcgcgcagc
      721 gccgtgagtg ggaccagtgg gacgacctgc atccgctgca tcaggcgttc gcggtgccgg
      781 gcgcgaagga ggggatcgac ccggctggcg tcgtgctgca cgacatcgac gcgcgcctcg
      841 gcgacggcgt catgctcgtg cgcacgccgg ggcacacgtc cgggaatcag accctgttct
      901 tcaagacgga caccggcatc tggggcgtct cggagaatgg ggtcagcgcc gacaactggt
      961 cgccgcaggc gagccgcatc cgcggcgtgc gcggccgggt cgggctgcac ggcctagagg
     1021 tcctgcccaa catgaacacg cccgagagca cggccgacca gtatctgtcg atgatcttcg
     1081 agcgcagcgt cgtcgacccg gcccgcggcg gcgacttcct gcagatgtac ccgtcgttcg
     1141 aggtcacgca ctcgtggctc acgccgggga tccgcgcgta catgcacggc gaactgcgct
     1201 tcggcgaggt cgctcgtcca ggcgcgtcgg cctgagacgg accggtcgct cgcccgctcc
     1261 gtcggcggat cagggctgca acgcgaccac ctgggcgcca tcgaggcact cacgcaccgc
     1321 ggccacctcc cagccgtcgt cggcccgcgc aggcgcggca ggggccgctc acttcttctt
     1381 cggcagcttg agcacggtgt cgatcacgtg gacgaccccg ttggtcgcct tgatgtcggc
     1441 cgatcgcact gacgacccgc cattgagctt cacgctgccg ctctcgttgc tgaccaggat
     1501 ctgcgggccg gcgacgctgc tgacgctcgg catctcggcg atctcggtcg ccgtcagggc
     1561 ggcgcccgag acgatgtgat agttgaggac gttcgccagc ttcttcttgt ccttgagcag
     1621 gcgatcgagc tcgggcttgg ggatcttggc gaaggcctcg tcgtcgggcg cgaacagggt
     1681 gaaccggccg ggccccttga gcgtgtcctt gaggcccgcc gcttcgatcg cgctcagcat
     1741 cgtcgtgaag ttgcccgcct ccgcgatcgt gtcgagcacg tccttggtcg cggccggcgg
     1801 cgccggtggc tcggcctccg cgggcatcgg ctcgggctcc ggcgccaccg tggcgtcgac
     1861 cgccatcggc tccggcggcg gcgcctcggt cggcggcgcg acgtcctgct tgggcttgct
     1921 cgcgcaggcg ccgagggcga cggcgagggc ggtgatcaac agagtcgggg cgtgcttcat
     1981 ggtgcgttca tcttctcagg tggagtcgcc gcgtggaccg gcgaggcggt cgaccagcgc
     2041 cttgagcttg gcacgttcgt tcgggacgag cagcccgccg cgggcctcga ccaggtcgcg
     2101 caccttctcg gcccccgcgc cgccgaccag ccacggggtc ttgccgcagg cctcgccgta
     2161 gccgtcgatc agctcgcgta cgcggtacgc tggcggaacg accgtcagtg acagaccgac
     2221 gagcgccggg tggacctcgg cgaccgcgtg acggatcgcg tgcggcggcg tgcgggcgcc
     2281 gagcagcacg gcgcgaaagt tccactcggt gaagcgcagc gcgaccccgt agagcggcac
     2341 cgcgtgctcc tcgtcggcga agcaggcgag cacgatcgtc cgctccgggg cctcgggctg
     2401 gctcaggcgc aggatgtagc gcagcgcgtt gccgatgacc tc
//
LOCUS       DASWNJ010001397         2246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_236232, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001397 DASWNJ010000000
VERSION     DASWNJ010001397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236232"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2246
                     /locus_tag="VGB85_23255"
     CDS             <1..>2246
                     /locus_tag="VGB85_23255"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HEY0137028.1"
                     /translation="SSARDTITDLHTSRGTSHAHDPDASHPVSSQPATSLILPISART
                     APALRTLAAAYRDRLRTANVLAADLCAAAALRRAHLEHRLAITGSTAAELITALDAYL
                     ADDLHPGWTQHGPGERPRLAFVFAGHSGHWVGMGRSLYAQVPVFRAALDACATALARH
                     RDGSLIDDLHATDERWASAEVLQPLVLALEVALAELWSDWGVLPQAVVGHSMGELAAA
                     CVCGALSLADAFRICVHRSRLIDSLRGRGAMASVALSLAAIDEHLAPYRDRVAVAAAN
                     SPQLTVISGDPAAVRELCDALSRKNIFTRPMRDATTAGHSPQLDPLLPELAAALTGLT
                     SQPARVPFHSTVTAGPLAGESLGPDYWLRNLREPVRFTQTIAGLGESGLDAFLEIGPN
                     PLLLGAIEQTLRPRGGALTRLGSLKADAPELPALHATLARLFVLGLDLEWSRIFSKGS
                     RAVDLPAYPWQRQRHWLAVATPTAPPSKSGLGQPVIAARAPHDRFWELRHADTAPVLH
                     IQGLPLLPIARPLELALAAARHLSPGTDTSLAAIGLTPTTLAETLDLQLIADDPAGPR
                     RGFELHARGPTPRLLASGRIDPPRATTLDPRHTDALATIQQRCASASPTLRATLCARG
                     LELASNLWAVDHASTGPARELLASVADAATPAILESALHLLASLVHEPGAAPVDGELF
                     LPHAIDHLWHAAKPAPIAWIHATLRGATGPSFGGTGDLRLLDITGRPVASLTGVHLQP
                     LAPGLARQ"
BASE COUNT          260 a         1042 c          657 g          287 t
ORIGIN      
        1 cctcgtccgc gcgcgacacg atcacggacc tgcacacctc gcgcggcacc tcgcatgcgc
       61 acgacccgga cgcctcgcac cctgtctcgt cgcagcccgc cacttccctg atactcccga
      121 tctccgctcg caccgccccg gccctgcgca ccctcgccgc cgcctaccgc gatcgtctac
      181 gcaccgcgaa cgtcctcgcc gccgacctgt gcgccgccgc ggccctgcgt cgcgcccacc
      241 tcgagcaccg cctcgcgatc accggcagca ccgccgccga gctgatcacc gcgctcgacg
      301 cctacctcgc cgacgacctg caccccggct ggacgcagca cggcccgggc gagcgcccgc
      361 gcctcgcctt cgtgttcgcc ggccacagcg gccactgggt cggcatgggc cgctcgctgt
      421 acgcgcaagt cccggtgttc cgcgccgccc tcgacgcctg cgcgaccgcc ctcgcccggc
      481 accgcgacgg ctccctgatc gacgacctcc acgccaccga cgagcgctgg gcctccgccg
      541 aggtcttgca gccgctcgtg ctcgccctcg aggtcgcgct cgccgagctg tggagcgact
      601 ggggcgtgct gccgcaggcg gtcgtcggtc acagcatggg cgagctcgcg gccgcctgcg
      661 tctgcggtgc cctctcgctc gccgacgcct tccggatctg cgtgcaccgc agccgcctga
      721 tcgacagcct gcgcggacgc ggcgcgatgg ccagcgtcgc cctctcgctc gccgcgatcg
      781 acgagcacct cgccccctac cgcgatcgcg tcgccgtcgc cgccgccaac agcccgcagc
      841 tgacggtcat ctccggcgac cccgcggccg tgcgcgagct gtgcgacgcg ctctcgcgca
      901 aaaacatctt cacccgcccg atgcgcgacg cgaccaccgc cgggcacagc ccgcagctcg
      961 acccgttgct gcccgagctc gccgccgcgc tgaccggcct cacttcccag cctgcccgcg
     1021 tcccgttcca ctcgaccgtc accgccggcc cgctcgcggg cgagtcgctc ggccccgact
     1081 actggctccg caacctccgc gagcccgtgc gcttcaccca gacgatcgcc ggcctcggcg
     1141 agtccggcct cgacgccttc ctcgagatcg gccccaaccc gctgctgctc ggcgcgatcg
     1201 agcagaccct gcgcccgcgt ggcggcgcgc tgactcgcct cgggtcgctc aaggccgacg
     1261 cccccgagct gcccgccctg cacgcgaccc tcgcccgcct gttcgtgctc ggcctcgacc
     1321 tcgagtggtc ccgcatcttt tccaagggca gccgcgccgt cgacctcccg gcctacccgt
     1381 ggcagcgcca gcgccactgg ctcgctgtcg cgacgccgac cgcccccccg agcaagtccg
     1441 gcctcggcca gcccgtgatc gccgcccgcg ccccgcacga ccgcttctgg gagcttcgcc
     1501 acgccgacac cgcccccgtc cttcacatcc agggcctgcc cctgctcccg atcgcgcgcc
     1561 ccctcgagct cgccctcgcc gcggcgcgcc acctgtcccc cgggacagac acctccctcg
     1621 ccgcgatcgg tctcaccccg accacgctcg cagagaccct cgacctccag ctgatcgccg
     1681 acgaccccgc cggccctcgc cgcggcttcg agctgcacgc ccgcggcccg accccgcggc
     1741 tgctcgccag cggccgcatc gatccgccgc gagccaccac gctcgacccg cgccacaccg
     1801 acgccctcgc cacgatccag cagcgctgcg cctccgcgag cccgaccctg cgcgcaacgc
     1861 tgtgcgcccg cggcctcgag ctcgccagca acctctgggc ggtcgaccac gccagcaccg
     1921 gccccgcgcg cgagctgctc gccagcgtcg ccgacgctgc caccccggcg atcctcgagt
     1981 ccgcgctgca cctgctcgcc tcgctggtcc acgagcccgg cgccgcccct gtcgacggcg
     2041 agctgttcct cccgcacgcg atcgaccacc tgtggcacgc cgcgaagccg gccccgatcg
     2101 cgtggatcca cgcgaccctg cgcggcgcga ccggcccgag cttcggcggc accggcgacc
     2161 tgcgtctgct cgacatcacc ggtcgcccgg tcgccagcct caccggcgtg cacctgcaac
     2221 cactcgcccc gggcctcgcc cgccag
//
LOCUS       DASWNJ010001398         1722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_273534, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001398 DASWNJ010000000
VERSION     DASWNJ010001398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273534"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VGB85_23260"
     CDS             complement(<1..305)
                     /locus_tag="VGB85_23260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY0137029.1"
                     /translation="MKIHEYLGKELLRSLGVPVPLSRVVFSPADARAAANELFKETGN
                     PVVVVKSQIHAGGRGKGRFKEHPDLGGVKVVKNADAAVETAEKMLGNTLVTIQTGPEG
                     "
     gene            complement(302..1273)
                     /gene="mdh"
                     /locus_tag="VGB85_23265"
     CDS             complement(302..1273)
                     /gene="mdh"
                     /locus_tag="VGB85_23265"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:TIGR01763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY0137030.1"
                     /translation="MSRRNKITIVGAGNVGATAAHWAAQKHLGDIVLVDIVQGFPQGK
                     ALDLAESAPVMGFDLKVVGTNGYDETADSDVVVITAGVPRKKDPATGKFTSRDELVEI
                     NRKIVGGVTREIASRSPNSVLICVSNPLDAMCHVMFHESGFPKERVIGMAGVLDTARY
                     KTFIAEALQVSVEDIHGMVLGGHGDTMVPLPSHTSVAGIPLTSLLSQEKIDAIIHRTR
                     NGGGEIVGLLGYSGYYAPAASTIAMVEAIVRDRKRVLPCAALLQGEYGYNDLFIGVPC
                     VLGAAGIEKIIEMELGDKEKGMLEVSANAVRDVVKILPYGKKDQAAT"
BASE COUNT          245 a          539 c          644 g          294 t
ORIGIN      
        1 ccctcggggc cggtctggat ggtgaccagc gtgttgccga gcatcttctc ggcggtctcg
       61 acggcggcgt cggcgttctt gacgaccttg acgccgccga ggtccgggtg ctccttgaag
      121 cggcccttgc cgcggccacc ggcgtggatc tgcgacttga cgacgacgac ggggttgccg
      181 gtctccttga acaattcgtt ggccgccgcg cgcgcgtccg ccgggctgaa cacgacgcgc
      241 gagagcggga cggggacgcc gagggagcgc agcagctcct tgcccagata ttcgtggatc
      301 ttcatgtggc ggcctggtcc ttcttgccgt aggggaggat cttgacgacg tcgcgcacgg
      361 cgttggccga gacctcgagc atgcccttct ccttgtcgcc gagctccatc tcgatgatct
      421 tctcgatgcc ggccgcgccg aggacgcagg gcacgccgat gaagaggtcg ttgtagccgt
      481 actcaccctg gagcagggcg gcgcacggca gcacgcgctt gcggtcgcgg acgatcgcct
      541 cgaccatcgc gatcgtggag gcggccgggg cgtagtagcc ggagtagccg agcaggccga
      601 cgatctcgcc gccgccgttg cgggtgcggt ggataatggc gtcgatcttt tcctgcgaca
      661 gcaagctggt cagcgggatg ccggcgaccg aggtgtgcga gggcagcggg accatggtgt
      721 cgccgtggcc gccgagcacc atgccgtgga tgtcctcgac gctgacctgc agggcctcgg
      781 cgatgaaggt cttgtagcgg gccgtgtcga gcacgccggc catgccgatc acgcgctcct
      841 tggggaagcc cgactcgtgg aacatgacgt ggcacatggc gtcgagcggg ttgctgacgc
      901 agatgagcac ggagttcggc gagcggctgg cgatctcgcg ggtgaccccg ccgacgatct
      961 tgcggttgat ctcgacgagc tcgtcgcgag acgtgaactt gccggtcgcg gggtccttct
     1021 tgcgcgggac accggcggtg atcacgacga cgtccgagtc ggccgtctcg tcatacccat
     1081 tggtgccgac gaccttgagg tcgaagccca tgaccggcgc cgactcggcg aggtcgagcg
     1141 ccttgccttg cgggaagccc tggacgatgt cgacgaggac gatgtccccg aggtgctttt
     1201 gcgcggccca gtgggcggca gtcgccccaa cgttgccggc tcccacgatc gtgatcttgt
     1261 tgcgtcgtga catgcgttta acttccccct atgaggtccg cgcctcgtat accagcgggc
     1321 ttatggcggg tgcaacgcca catgcgcggc cggacgggca catcgcgggc cccgcgggga
     1381 cctgcggcgc ctgtgtgtgt gggcacactc gcgcgttggt ccggggacgg tgcgagatcg
     1441 cgcaaaccgc gggcggggcg tcggcggcgg acgccgggag cgaccctggc gtccgcgcga
     1501 tcgtggggcg caggctggcc ccggggacag gtcccgaagg ccaggtgagc accggcgggt
     1561 gatgctcgcc gggtcgcgcg acgatcagat gtcgtcggcg aggcgcaggc cgcaggtctg
     1621 cgtcaggtcg caggcggtgc agctccagcg gaagttgtcg acgaggatcg cggagtcgat
     1681 cgagccgtcc tcgaggtcca tgatcgcgag cgtcagggtg ag
//
LOCUS       DASWNJ010001399         2236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_308463, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001399 DASWNJ010000000
VERSION     DASWNJ010001399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308463"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VGB85_23270"
     CDS             complement(<1..739)
                     /locus_tag="VGB85_23270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137031.1"
                     /translation="MLVPPVDRMPYRTSPRLALAGLALLSTLLLQLGCATGTEKMVRG
                     GIEGGLAGTLEALNDPHNKELLRKILQDSDIKQSAHDLTEALTGGALDGVTDAERQKK
                     IREASDAYIRTIADAVGKALDEDISPSVTRGVGDVVGGTIASALRPENRQLARSFIDG
                     VTRSTITAFMQSTSAGLRDDLGPAINKVLAEDLGPGLQRVIEDNLGPAIKTVMERDLQ
                     PMMASALGGEEGGGAGTFARALTRQIVL"
     gene            complement(771..1895)
                     /locus_tag="VGB85_23275"
     CDS             complement(771..1895)
                     /locus_tag="VGB85_23275"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY0137032.1"
                     /translation="MTPEQTEAVEIKNTDRREQARWLILLASMVGALYLCWLMLVPFV
                     NVLLWAGVLTLSFMPFHLRILKRTGKPNLSAAISTLFVVFVIGVPMGLVAWAIIREIV
                     PAIAMVQTAFATFLDPDATGPAMAWLGERVDLPRTRLQINEQLGAMSTAIASRSMGIV
                     GGVLGVLIQALFVVFVMFYLFRDGKMIRDAMASAIPLRNRETRVILGRVTEVVGASVN
                     GVLVIAAIQGTLGGAAFAALGLPSAIVWGAVMIFLSLIPVAGAFVIWIPAAIYLAVTG
                     SYIKAIILTLWGGVVISLVDNFLRPRLVGKRAKLHELFIFFAVLGGLHVFGLVGLVLG
                     PVVLAIALSLFETFRHPHASSSMTATDMILPSDSPYRVRE"
     gene            complement(1997..>2236)
                     /locus_tag="VGB85_23280"
     CDS             complement(1997..>2236)
                     /locus_tag="VGB85_23280"
                     /inference="COORDINATES: protein motif:HMM:NF023425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ice-binding family protein"
                     /protein_id="HEY0137033.1"
                     /translation="QVAGDLDVSTGKHVLLAGGARANNVFWQVAGQATIHANAHFEGI
                     ILAKTDIIFQTKASLHGRALAQTMVAFDKNVVTVP"
BASE COUNT          363 a          708 c          831 g          334 t
ORIGIN      
        1 cgagcacgat ctggcgggtc aaggcgcggg cgaaggtccc cgcgccaccg ccctcctcgc
       61 cgccgagggc cgaggccatc atcggctgga ggtcgcgctc catcacggtc ttgatcgcgg
      121 ggccgaggtt gtcctcgatg acgcgctgca ggccggggcc gaggtcctct gcgagcacct
      181 tgttgatggc ggggccgagg tcgtcgcgca ggccggccga ggtgctctgc atgaacgcgg
      241 tgatcgtcga gcgggtgacg ccgtcgatga agctgcgcgc gagctggcgg ttctcggggc
      301 gcagcgcgga ggcgatcgtg ccgccgacga cgtcgccgac gccgcgggtg acggagggcg
      361 agatgtcctc gtcgagggcc ttgccgacgg cgtcggcgat cgttcggatg taggcgtcgc
      421 tcgcctcgcg gatcttcttt tgccgctcgg catcggtgac gccgtcgagg gcgccgccgg
      481 tcagcgcctc ggtcaggtcg tgggcggact gcttgatgtc gctgtcctgc aggatcttgc
      541 gcagcagctc cttgttgtgc gggtcgttga gggcctcgag ggtgccggcg aggccaccct
      601 cgatgccgcc gcggaccatc ttctccgtgc cggtcgcgca gccgagctgc aacaggagcg
      661 tcgacaggag ggcgaggccg gcgagggcga ggcggggaga cgttcgatac ggcatacgat
      721 ccacgggcgg tacaagcatg cgggaggtcc tcaggaacgc cagcgcggtc tcactcgcgg
      781 acgcgatacg ggctgtcgct gggcaggatc atgtccgtgg cggtcatgct gctgctggcg
      841 tgggggtggc gaaaggtctc gaacagggac agcgcgatcg ccaggaccac cgggccgagc
      901 acgaggccga cgaggccgaa cacgtgcagg ccgccgagca cggcgaagaa gatgaacagc
      961 tcgtgcagct tggcgcgctt gccgaccagg cgcgggcgca ggaagttgtc gacgaggctg
     1021 atgacgacgc cgccccacag ggtcaagatg atcgccttga tgtacgagcc ggtgacggcg
     1081 aggtagatcg cggcggggat ccagatgacg aaggcgccgg cgaccgggat cagcgacagg
     1141 aagatcatga cggcgcccca gacgatcgcg gagggcaggc cgagcgcggc aaaggccgcg
     1201 ccgccgagcg tgccctggat cgcggcgatc acgaggacgc cgttgacgct ggcgccgacg
     1261 acctcggtga cgcggccgag gatcacgcgg gtctcgcggt tgcgcagcgg gatcgcggag
     1321 gccatcgcgt cgcggatcat cttgccgtcg cggaacaggt agaacatcac gaacacgacg
     1381 aacagggcct ggatgagcac gccgaggacg ccgccgacga tccccatgct gcggctggcg
     1441 atcgccgtgc tcatcgcgcc gagctgctcg ttgatctgca ggcgcgtgcg cggcaagtcg
     1501 acgcgctcgc cgagccaggc catcgccggg ccggtcgcgt cggggtcgag gaaggtggcg
     1561 aaggcggtct ggaccatggc gatcgcgggc acgatctcac ggatgatcgc ccacgcgacc
     1621 agacccatcg ggacgccgat cacgaacacg acgaacagcg tcgagatggc ggcgctgagg
     1681 ttcggcttgc cggtgcgttt gaggatccgc aggtggaacg gcatgaacga cagggtgagg
     1741 acgcccgccc acagcaggac gttgacgaac ggcaccagca tcagccagca caggtagagc
     1801 gcgcccacca tcgaggcgag caggatcaac cagcgcgcct gctcgcgccg atcggtgttc
     1861 ttgatttcga ctgcctcggt ctgctctggg gtcatctacg ctgctcctgc gttcgcgttt
     1921 cgacggcgac cacgggcccg cgacgagttg cgccgcggac cccccgaacc cggctgcaca
     1981 cggcccccgg tcggcttcac gggaccgtga cgacgttctt gtcgaacgcg accatggtct
     2041 gtgcgagtgc ccggccgtgc agcgaggctt tggtctggaa gatgatgtcc gtcttggcga
     2101 ggatgatacc ctcgaagtgc gcattcgcgt gaattgtagc ctgtccggcg acctgccaaa
     2161 acacgttgtt cgcccgggcg ccgccggcca gcaacacgtg cttgccggtg ctgacatcga
     2221 ggtcgcccgc gacctg
//
LOCUS       DASWNJ010001400         4704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_129690, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001400 DASWNJ010000000
VERSION     DASWNJ010001400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129690"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..1126
                     /locus_tag="VGB85_23285"
     CDS             62..1126
                     /locus_tag="VGB85_23285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137034.1"
                     /translation="MPTLSVRPLACALLLTACPGDGGTGDTGSTGSTITSASTVEPTS
                     TPTTGTGATDTSATDTSATDGTATQATGDATTLPATDTSATTTDGLDTGSTGATGTSG
                     TTTDASTTDASTTETGTTGPQEASEIEVVITADNAYGFAYGTETDIAKYYGGLEAVTA
                     GQIFNCGEGPETYVIPAQEAEGATHLYIIAWADSAVTQGVLARFRRLGGGGGFGEDVF
                     TGTKGWEVCATGIDYEPGDGGPPLAQINEYIGKCNGGLLDPNTTSVGWVDEIGTQYGS
                     VAFGEDNTTPYEGGSPQPGNEFQLVCPDVMPAEAHWMWFNWANLKPPESPFLWPGEDG
                     NPDHQFLIFRLAAELLPEPQ"
     gene            complement(1153..2085)
                     /locus_tag="VGB85_23290"
     CDS             complement(1153..2085)
                     /locus_tag="VGB85_23290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137035.1"
                     /translation="MENFVPFLTGELGLDVEETVRYSWLDEGTYPDSTCPEGSAGCQI
                     EEHAYARKPTLLHELIHAVTYVNGMNHLSFFTEGLAVAYDPFNGAAPGPRYRIYPAEG
                     EETRDPRDFMTGKLGVQGYDIAGSFVTFLLMRHGPEKIVSLTRRLDLESSLQEVRAHF
                     LEVYGVDMDDEVELYKMNGPCEDLVFEVRPYDCAMPLVPWDESAWSWSASMVCDDDSV
                     AGGVDPDRDVNSVRSVALVVPRTGRYVVKLDADGDAHVTLGRCFGCPWAPADSVLQSA
                     EALEVELSAGAHFVRVTALAAEEPTVRVTLVPAE"
     gene            complement(2264..3805)
                     /locus_tag="VGB85_23295"
     CDS             complement(2264..3805)
                     /locus_tag="VGB85_23295"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0137036.1"
                     /translation="MHFNTVKILILAVALGGCQKQQGTSTPPVKDTGTSTPAAEQKAT
                     SDRQAQEAAAKRAQLAALPPLPGVEAARAVKFPEPQIQTLPNGLELVVLEDHEVPKLR
                     VSAFIKAGHIYAPGDQPSLAEFTLLLLGEGTKKRDKASFDAQVDATGGDIIAAIDDEV
                     AGVTADVLARDASFAFTAVAEQVMQPALPETSLKKIKDQLLQGVDVQKASPFGLSSRM
                     AARAIYGEESPYGRPFPTTPQIEGMTREQALAFHAKHYQPGNAMLVVAGDITPRQARK
                     LADRAFGTWRAGQAVPVPKAAKRPLPAAAPVVYIVDRKASAQASVLALVAAPGIGEPG
                     WLQIRVLQQLLGGGLSGRLNMVLREQLGLTYGAGAFHSFGYDGGAFFAGGSTKTKSAA
                     EFTQALVNLLRQPSSEAVEAVELRRLLSKISGQFALEVEGMDTVSMKTVQQRLFGLPP
                     DFWERYRSDVESISADDLKRTTQSLLVDQAVQIVVVGRADKLRKELAGFGEVRVYDTD
                     LKRID"
     gene            complement(3815..>4704)
                     /locus_tag="VGB85_23300"
     CDS             complement(3815..>4704)
                     /locus_tag="VGB85_23300"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEY0137037.1"
                     /translation="KEEVRQQQENSPYAKTLLLDWPAVAYSSWEYAHSMYGSMEDLSN
                     APVQAFVDFFKKYYVPNNATLVICGDFEPRRARALVEKYYGPLQAGPEIPRTFPQEAK
                     QTEAVYRTVSDPLAPTPLALISFDIPPPQTPDHDALELLATVLTGGASARLNKLLIDD
                     KKLASTVMMQPGFPWATYGPSQMVSLLIASKDSKLEDLRAAFWTELEKIQKEGVSSQE
                     ISRARSKLYKRHIDGLGNTLYKAGQLATYEAFFGGAEKFTDDLRRFDKVTAADLKRVA
                     TQYLGKPASVTFDILPGKT"
BASE COUNT          765 a         1682 c         1498 g          759 t
ORIGIN      
        1 caggcccggc gcccgggccg ctggcctcca gccggcgagg atcggccgct atcatccgtg
       61 gatgccaact ttgtccgtgc gtcccctcgc ctgcgccctg ctgctgaccg cctgccccgg
      121 cgacggcggc accggcgaca ccggctcgac cggctccacg atcaccagcg cgagcaccgt
      181 cgaaccgacc tcgacgccga ccaccgggac cggcgccacc gacaccagcg ccaccgacac
      241 cagcgccacc gacggcaccg ccacgcaggc caccggcgat gcgacgacgc tgcccgcgac
      301 cgacacctcg gccaccacca ccgacggcct cgacaccggg agcaccggcg cgaccgggac
      361 gtccggcacc accaccgacg cgagcaccac cgacgcgagc accaccgaga cgggcaccac
      421 cggcccgcag gaggccagcg agatcgaggt cgtcatcacc gccgacaacg cctacggctt
      481 cgcgtacggg accgagaccg acatcgccaa gtactacggc ggcctcgagg ccgtcaccgc
      541 cggacagatc ttcaactgcg gcgagggccc ggagacctac gtcatcccgg cgcaggaggc
      601 cgagggcgcg acgcacctct acatcatcgc atgggcagac tccgcggtga cgcagggcgt
      661 gctggcccgc tttcgccggc tcggcggcgg cggcggcttc ggcgaggacg tgttcaccgg
      721 caccaagggc tgggaggtgt gcgccaccgg catcgactac gagcccggcg acggcggccc
      781 cccgctcgcg cagatcaacg agtacatcgg caagtgcaac ggcggcctgc tcgacccgaa
      841 caccaccagc gtcggctggg tcgacgagat cggcacgcag tacggctccg tcgccttcgg
      901 cgaggacaac acgaccccgt acgagggcgg ctccccgcag ccgggcaatg aattccagct
      961 cgtctgcccc gacgtcatgc ccgccgaggc tcactggatg tggttcaact gggccaacct
     1021 caagcccccg gagagcccct tcctgtggcc cggcgaggac ggcaaccccg accaccagtt
     1081 cctgatcttc cgcctcgcgg ccgagctgct gcccgagccg cagtgaggcc ggcgccatga
     1141 cgaggcgccg cttcattcgg caggaacgag ggtgactcgc acggtcggtt cttccgcggc
     1201 cagggcggtc actcgcacga aatgtgcgcc ggcggagagc tcgacctcga gcgcctccgc
     1261 gctttgcagg acactgtcgg ccggcgccca tgggcacccg aagcagcgcc cgagggtcac
     1321 atgagcgtcg ccgtcggcat cgagcttcac gacgtagcgg ccggtccgcg gcacgacgag
     1381 cgcgaccgag cgaacgctgt tgacgtcgcg gtcggggtcg acgccgccag ccacactgtc
     1441 gtcgtcacaa accatggagg ccgaccagga ccaggcagac tcgtcccagg gcacaagcgg
     1501 catggcgcag tcgtacggcc gcacctcgaa cacgagatcc tcgcaaggcc cgttcatttt
     1561 gtacagctcg acttcgtcgt ccatatcgac gccgtagacc tccagaaagt gcgctcttac
     1621 ctcttgcaaa gaactctcga ggtccagccg ccgggtcaat gagacaattt tctcgggccc
     1681 atgccgcatg agcaaaaatg tcacaaaact gccagcgatg tcgtagccct ggacacccag
     1741 tttgccggtc atgaagtcgc gcgggtccct cgtctcttct ccctccgccg gataaatccg
     1801 gtagcgtggc cctggagcag ccccattgaa tggatcgtac gcgactgcga gcccctctgt
     1861 aaaaaacgac agatggttca ttccattcac gtacgtcact gcatggatca actcgtgcag
     1921 aagcgtgggc ttgcgtgcgt aagcgtgctc ttcaatctgg catcctgcgc tcccctccgg
     1981 gcatgtggag tcaggatagg tcccctcgtc gagccacgag taacggacag tctcttcgac
     2041 atcgagcccg agttctcccg tcaggaacgg cacgaagttc tccatgaagg gaactgtacc
     2101 ggcacagact cgcaaggatg gactcgcctc gtaccgaaca tgtcccgtgt cgcctggggt
     2161 acaggacatg acgagacaaa gcgccataat ggagccccac gccgaaccag ccgaacggcg
     2221 ccctggctcc ccccacccgt ccccccgcgg actgctcacc ggactaatca atccgcttga
     2281 ggtcggtgtc gtacacccgc acctcaccga atcctgccag ctccttgcgc aatttatcgg
     2341 cccgcccgac gacgacgatc tgcaccgcct gatcgaccag cagcgactgc gtcgtccttt
     2401 ttaaatcgtc ggccgagatc gactcgacgt cgctgcgata tctctcccag aagtccggcg
     2461 gcagcccgaa cagccgctgc tggacggtct tcatcgacac ggtgtccatg ccctcgacct
     2521 cgagcgcgaa ttgtccggag atcttgctca gcaatcgccg cagctcgacc gcctcgaccg
     2581 cctcgctgct cggttggcgc agcaggttca ccagcgcctg cgtgaactcg gccgcgctct
     2641 tggtcttggt gctgccgccg gcgaagaacg ccccgccgtc gtagccgaag ctgtggaacg
     2701 cgcccgcgcc gtaggtcagg ccgagctgct cgcgcagcac catgttgagg cgccccgaca
     2761 gcccgccgcc gagcagctgc tgcagcacgc ggatctgcaa ccagcccggc tcaccgatgc
     2821 cgggcgcggc caccagggcg agcaccgagg cctgcgcgct cgccttgcgg tcgacgatgt
     2881 acacgaccgg cgccgccgcc ggcagcggcc gcttcgccgc cttcggcacc ggcaccgcct
     2941 gtcctgcgcg ccatgtccca aaagccctgt ccgcgagctt gcgggcctgc ctcggcgtga
     3001 tgtcgcctgc caccaccaac atcgcgttgc cgggctggta gtgcttcgcg tggaacgcca
     3061 gcgcctgctc gcgggtcatg ccttcgatct gcggcgtcgt cgggaacggg cgcccgtacg
     3121 ggctctcctc gccgtagatc gcccgcgccg ccatgcgcga cgacagcccg aagggcgacg
     3181 ccttctgcac gtcgacccct tgcagcagct ggtccttgat tttcttgagc gacgtctccg
     3241 gcagggccgg ctgcatcacc tgctcggcga ccgccgtgaa cgcgaagctc gcgtcgcggg
     3301 cgagcacgtc ggcggtcacg cccgcgacct cgtcgtcgat cgccgcgata atgtcaccgc
     3361 cggtcgcgtc gacctgcgcg tcgaagctcg ccttgtcgcg cttcttggtg ccctcgccga
     3421 gcagcagcag cgtgaactcg gccaggctcg gctggtcgcc gggcgcgtag atgtggccag
     3481 ctttgataaa tgcgctgacg cgcagcttgg gcacctcgtg gtcctcgagc acgacgagct
     3541 cgagcccgtt gggcagcgtc tggatctgcg gctcggggaa cttgacggcc cgcgccgcct
     3601 cgacgcccgg caacggcggc agcgcggcga gctgcgcccg cttggccgcc gcctcctggg
     3661 cctggcggtc gctggtcgcc ttctgctcgg cggcgggcgt gctcgtgccg gtgtccttga
     3721 ccggcggcgt gctcgtgccc tgttgcttct gacagccgcc cagcgcgacg gccaaaatca
     3781 agatcttcac ggtattgaag tgcatggtcg gatctcaggt cttgccgggg aggatgtcga
     3841 aggtcacgct ggcgggtttg ccgaggtact gggtcgcgac gcgcttgagg tcggcggcgg
     3901 tgaccttgtc gaagcggcgc aggtcgtcgg tgaacttctc ggcgccgccg aagaacgcct
     3961 cgtacgtggc gagctggccg gccttatata aagtgttgcc gaggccgtcg atgtggcgct
     4021 tgtacagctt ggagcgcgcg cgcgagatct cctgcgagct cacgccttct ttctggatct
     4081 tctccagctc ggtccagaag gccgcgcgca ggtcctcgag cttgctgtcc ttgctggcga
     4141 tcagcaggct gaccatctgg ctcggtccgt aggtggccca ggggaagccg ggctgcatca
     4201 tgacggtgct cgccagcttt ttatcgtcga tcagcagctt gttgagccgc gccgaggcgc
     4261 ccccggtaag caccgtcgcc agcagctcga gcgcgtcgtg gtccggcgtc tgcggcggcg
     4321 ggatgtcgaa ggagatcagc gcgagcgggg tcggggccag cgggtcggac accgtgcggt
     4381 acaccgcctc ggtctgtttg gcctcctgcg ggaacgtgcg cgggatctcc gggccggcct
     4441 gcagcggccc gtagtacttc tcgaccaagg cccgcgcgcg cctgggctcg aagtcgccgc
     4501 agatcaccag cgtcgcgttg ttcggcacgt agtatttttt aaagaaatcg acgaaggcct
     4561 gcaccggggc gttcgagagg tcctccatcg atccgtacat cgaatgcgcg tactcccacg
     4621 acgagtatgc gaccgccggc cagtcgagca gcagcgtctt ggcgtagggc gagttctcct
     4681 gctgctggcg gacctcctcc ttga
//
LOCUS       DASWNJ010001401         4476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_376702, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001401 DASWNJ010000000
VERSION     DASWNJ010001401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376702"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="VGB85_23305"
     CDS             complement(<1..1128)
                     /locus_tag="VGB85_23305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0137038.1"
                     /translation="MATIKKKALVIGAGTGGYPCAIRLGQLGVDAMLVEKEKPGGVCL
                     NWGCIPSKALISATKLYHKAQHVKDMGLSFGQPAVDMVQMQAWKGGIVKKLTGGVQSL
                     VKGAGTTYVKGTAEFLDAHRVKITYADGKPEDIIEAEHIVIATGSVPIEIPGFKYDHK
                     RVLDSTSALDLTEIPKRMVCVGGGIIGLELGQTFQRLGTQLIVLEGLPRILNGVDPES
                     AEVVARQIKKDGGQIHTGAKALGWEERNGEAVVKAEVGGKVQEFPCDVILVAVGRRPV
                     TKGFGLEKTGVKVNERGFIVVDNRQATNVPGLYAVGDVVGQPMLAHKASHEGEVVAEV
                     IAGKKTINDARQIPNVVFTEPEIASSGLTVEEAEAKGRKVNI"
     gene            complement(1159..2490)
                     /locus_tag="VGB85_23310"
     CDS             complement(1159..2490)
                     /locus_tag="VGB85_23310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEY0137039.1"
                     /translation="MLEFKLPEIGEGVVEGEIVRWHKQPGDFVQANEALVEVMTDKAT
                     IEVPSPVSGTVRELLAQIGDICAVEQIIAVLDLASSAGKAPVASAPAAPPAASSSSSS
                     SGPVLATPAARALARESGIDLHSLPTDDRGRITKGDVLRAQQDIGKAAELYAGANSEP
                     APVRPAPVIEVAAAYVAPSKPAAPAAKPAAPAAKPAAAKPAPRPVVTAEGDEEIPFRG
                     MRRKISEALVRSYTEAVHYTYVEQVNVTKLVKLRADAKSVAAEQGVSLTFLPFIVKAV
                     VHALKKYPIVNAELDEAGGRIVVKKRYNIGVAAATEQGLMVPVVHGADRMSILDLGRE
                     IARLGEAARTGACSREELTGGTFTISSLGNIGGLLATPIINYPEVGILGVHAIRKTPI
                     VDDRDNIVIGHLMNLSVSLDHRVVDGFEGAQFLQEVRRYLEDPNLLLLASM"
     gene            complement(2503..3465)
                     /locus_tag="VGB85_23315"
     CDS             complement(2503..3465)
                     /locus_tag="VGB85_23315"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0137040.1"
                     /translation="MNVLERLSEAMAELLRDDLRRVLLGEDVREGGMLGLSRATMQDP
                     ALGLRVLSTPLTPAASLAHATGLALAGRRPIVLLASATALLEGYAALRELGRISSTAG
                     GERSAPVLIVAPTGPGFGLGGDGADSPEAALTAVPGLRVVVLSDALEAVAMLRSAASF
                     DDEETPTVLLLPRALVLRELMADDFKPALGRPLGASRVVREGAAATVFAIGAAVDQAF
                     AAVEATGLEVTIVEVSGLAPLDEPGILAAATGGKLVVTHPGGRAHGPGAELAALLADR
                     AILHLDAPVLRVTGAAGPFTGRDEFAGLPDVEAIAAAITRVATY"
     gene            complement(3462..4415)
                     /locus_tag="VGB85_23320"
     CDS             complement(3462..4415)
                     /locus_tag="VGB85_23320"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY0137041.1"
                     /translation="MEPSPWDPEGVEHSLDPEGQLLDRRAAHGLDLRALYKRLVAARL
                     LDLRLSRLGLPMWVSSAGEEAATVLSATLAGPEDWLYPSVRDLALAPIRGMDVDEFAM
                     QVLGGTYNGRPGAIAAADLGIGHAPETLGLHLALAAGQAHAQKLAGTGRMTAALFGEG
                     LTTTSMFHESVALAVACDLPLVFVCKSQLWPDGAPAEAGLLGDSVAERVKACGLWSRR
                     VDGADVVGVYAALDQAFRRARDGRGPGLVEVVATRLQTGEQSTDVPAHRDPLERLRRH
                     LDREKQWTPTFQDVIEAEVRGVLERAFSHAQKQAAEREGSA"
BASE COUNT          615 a         1614 c         1568 g          679 t
ORIGIN      
        1 gatgttgacc ttgcggccct tggcctcggc ctcctcgacg gtgagccccg aggaggcgat
       61 ctccggctcg gtgaacacga cgttggggat ctgccgcgcg tcgttgatcg tcttcttgcc
      121 ggcgatcacc tcggcgacca cctcgccctc gtgcgaggcc ttgtgcgcga gcatcggctg
      181 gccgacgaca tcgccgaccg cgtagaggcc gggcacgttg gtcgcctggc ggttgtcgac
      241 gacgatgaag ccgcgctcgt tgaccttgac gcccgtcttc tcgaggccga agcccttggt
      301 caccggacgg cgaccgacgg cgacgaggat gacgtcgcag gggaactcct ggaccttgcc
      361 gccgacctcg gccttgacca cggcctcgcc gttgcgctcc tcccagccga gcgccttggc
      421 gccggtgtgg atctgtccgc catccttctt gatctgacgc gcgacgacct cggcggactc
      481 ggggtcgacg ccgttgagga tccgcggcag gccctcgagc acgatcagct gcgtgccgag
      541 gcgctggaag gtctggccga gctcgaggcc gatgatgccg ccgccgacac acaccatgcg
      601 cttcgggatc tcggtgaggt cgagcgcgct ggtgctgtcg agcacgcgct tgtggtcgta
      661 cttgaagccg gggatctcga tcggcaccga accggtggcg atgacgatgt gctcggcctc
      721 gatgatgtcc tccggcttgc cgtccgcgta ggtgatcttc acgcggtgcg cgtcgaggaa
      781 ctcggccgtg cccttgacgt acgtggtgcc cgcgcccttg accagcgact gcacgccgcc
      841 ggtcagcttc ttgacgatgc cacccttcca cgcctgcatc tggaccatgt cgaccgcggg
      901 ctgcccgaag ctcaggccca tgtccttgac gtgctgggcc ttgtgataca gcttggtcgc
      961 gctgatcagc gccttcgacg ggatacagcc ccagttgagg cacacgccgc ccggcttctc
     1021 cttctcgacg agcatggcgt cgacgcccag ttgaccgaga cggatcgcgc agggatatcc
     1081 acccgtgcct gcgccgatga cgagcgcctt cttcttgatc gtggccatgg tcttcctttt
     1141 cgtaagcccc gttgggggct acatgctcgc gagcagcagc aggttcgggt cctcgaggta
     1201 gcgccgcact tcctgcagga actgcgcgcc ctcgaagccg tcgacgacgc gatggtcgag
     1261 cgacacggag aggttcatca ggtgaccgat gacgatgttg tcgcggtcgt cgacgatcgg
     1321 cgtcttgcgg atcgcgtgga ccccgaggat gccgacctcg ggatagttga tgatcggggt
     1381 cgccagcagg ccgccgatgt tgccgaggct ggaaattgtg aatgtgccgc cggtcagctc
     1441 ctcgcgcgag catgcgcccg tgcgcgccgc ctcgccgagc ctggcgatct cgcgcccgag
     1501 gtcgaggatg ctcatgcggt ccgcgccatg caccaccggc accatcaggc cctgctcggt
     1561 cgccgccgcc acgccgatgt tgtagcgctt cttgacgacg atccgaccgc ccgcctcgtc
     1621 gagctccgcg ttgacgatcg ggtatttttt cagggcgtgg acgaccgcct tcacgatgaa
     1681 gggcaaaaag gtcaggctga cgccctgctc ggccgcgacg ctcttggcgt ccgcgcgcag
     1741 cttcaccaat ttggtgacgt tgacctgctc gacgtaggtg tagtgcaccg cctccgtgta
     1801 cgagcgcacc agcgcctcgg agatcttgcg acgcatgccc cggaagggaa tctcctcgtc
     1861 gccctcggcg gtgaccaccg ggcgcggcgc aggcttggcg gccgcgggct tggctgccgg
     1921 cgcggctggc ttggctgctg gcgcggccgg cttgctcggc gcgacgtagg cggccgcgac
     1981 ctcgatcacc ggggccgggc gcaccggcgc gggctcgctg ttcgcacccg cgtacagctc
     2041 ggccgccttg ccgatgtcct gctgggcgcg aagaacatca cccttggtga tgcggccgcg
     2101 gtcgtcggtc ggcagcgagt ggaggtcgat cccggactcg cgcgcgagtg cccgcgccgc
     2161 gggggtcgcg agcacggggc cgctcgaaga tgaggaggat gaggccgctg gcggagcagc
     2221 cggcgcgctg gccacggggg ccttgccggc ggacgacgcg aggtcgagca ccgcgatgat
     2281 ctgctcgacc gcgcagatgt caccgatctg ggcgagcagc tcgcggaccg tcccggagac
     2341 cggcgagggc acctcgatcg tcgccttgtc ggtcatcacc tcgacgagcg cctcgttggc
     2401 ctgaacgaag tcgccgggct gcttgtgcca gcggacgatc tcgccctcga ccacgccctc
     2461 accgatctct ggaagcttga actcgagcat gcacaggtcc tgtcagtagg tggcgacccg
     2521 ggtgatcgcg gcggcgatcg cctcgacgtc cggcaggccc gcgaattcgt cgcggcccgt
     2581 gaagggtccc gctgcgccgg tcacccgcag caccggcgcg tcgaggtgga ggatcgcccg
     2641 gtcggcgagc agggcggcca gctcggcgcc gggtccgtgc gcgcggccgc cggggtgcgt
     2701 gaccaccagc ttgccgccgg tcgccgccgc gaggatcccc ggctcgtcga gcggcgcgag
     2761 gccgctgacc tcgacgatcg tgacctcgag gcccgtcgcc tcgaccgccg cgaacgcctg
     2821 gtcgaccgcc gcgccgatcg cgaacaccgt cgccgccgcg ccctcgcgca cgacccggga
     2881 cgcgccgagc gggcgaccga gcgccggctt gaagtcgtcg gccatcagct cgcgcagcac
     2941 cagcgcgcgc ggcagcagca gcaccgtcgg cgtctcctcg tcgtcgaagc tcgccgcgga
     3001 ccgcagcatc gccacggcct ccagcgcgtc gctcaggacg acgacgcgca ggcctggcac
     3061 ggccgtcagc gcggcctcgg gcgagtcggc cccgtcgccg ccgagcccga agcccggacc
     3121 cgtgggggcg acgatcaaca ccggggccga ccgctcaccg ccggcggtcg agctgatgcg
     3181 cccgagctcg cgcagcgccg cgtagccctc gagcagcgcc gtcgccgagg cgagcagcac
     3241 gatcggccgg cgtccggcga gcgccaggcc ggtcgcgtgc gccagcgagg ccgcgggcgt
     3301 cagcggggtc gacagcacgc gcaggcccag cgcggggtcc tgcatggtcg cgcgcgacag
     3361 cccgagcatg ccgccctcgc gcacgtcctc gccgagcaac acgcgacgaa gatcatcgcg
     3421 cagcagctcg gccatcgcct ccgacaatcg ctcgagtacg ttcatgccga tccctcgcgc
     3481 tccgcggcct gcttctgcgc gtgactgaaa gctcgctcga gcacgccgcg gacctccgct
     3541 tcgatgacgt cctggaaggt cggcgtccac tgcttctcgc gatcgaggtg ccggcgcagg
     3601 cgctcgagcg ggtcgcggtg ggccggcacg tccgtgctct gctcgccggt ctgcaggcgc
     3661 gtcgccacga cctcgacgag gcccggaccg cggccgtcgc gggcgcgacg aaacgcctgg
     3721 tcgagcgccg cgtacacgcc gacgacatcc gccccgtcga cccggcgcga ccacagcccg
     3781 cacgccttga cgcgctcggc cacgctgtcg ccgagcaggc cggcctccgc gggcgcgccg
     3841 tcgggccaca gctggctctt gcacacgaac acgagcggca ggtcgcaggc gaccgcgagc
     3901 gcgaccgact cgtggaacat cgaggtcgtg gtcaggccct cgccgaacag cgcggcggtc
     3961 atgcgcccgg tgccggcgag cttctgcgcg tgcgcttgcc cggcggcgag cgccaggtgc
     4021 aggccgaggg tctccggcgc gtggccgatg ccgaggtcgg ccgcggcgat cgcccccggc
     4081 cggccgttgt acgtcccgcc gagcacctgc atcgcgaact cgtcgacgtc catcccgcgg
     4141 ataggcgcga gcgcgaggtc tcgcacgctg ggatacagcc agtcctcggg gccggcgagg
     4201 gtcgcgctga ggaccgtcgc cgcctcctca cccgcgctcg agacccacat cggcaagccg
     4261 agccgcgaca ggcggaggtc gagcaggcgg gccgccacga ggcgcttgta cagcgcgcgc
     4321 aggtcgaggc catgcgccgc tcgccggtcg aggagttgac cctcggggtc gaggctgtgc
     4381 tccacacctt cgggatccca gggagacggt tccatcgcca cggtggtcac gggttgtatc
     4441 tgccgcccaa aaccccgtca aggccggaat tcccgc
//
LOCUS       DASWNJ010001402         3945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_695003, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001402 DASWNJ010000000
VERSION     DASWNJ010001402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695003"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VGB85_23325"
     CDS             complement(<1..444)
                     /locus_tag="VGB85_23325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137042.1"
                     /translation="MSPLTNLRTSPLNLPLACVVALSLLAPAAAAAPGRAYGPVLVNM
                     FPGPAPAGVSASGHLYGTVLANVSLGPASTGASPPAPTSEPAKAACPAAQEAIAGTSA
                     DKYGEAARQFEQCARTSGDAGLWKKAGMARYSARQYAHAIQALDIY"
     gene            complement(453..1400)
                     /locus_tag="VGB85_23330"
     CDS             complement(453..1400)
                     /locus_tag="VGB85_23330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0137043.1"
                     /translation="MADLDGDDEVDRLTPAEQRLVLRGGVAAVVALAGALVWSIVAAP
                     VIEQHVPVAPLTTSPQARAITPEVLAASPSAAPAGVRRVAEVTPGRRLPGLFGLQLGF
                     LRSNDIIGTIALNEVLRGTPAVKVVNLWATWCAPCVREIGMFRALSEGWRRDIRFVPI
                     HIGAVNDGDAYRRLVDQMPATSAEPLIDASGDAFQGLLRGAGLLETGEGIPITLLLDC
                     RNELRWIHVGDLADTAALSERVSALRGELGSPRCAPPEPPPSALPSPPGCGDGTCDGG
                     EHCGNCLADCGCIVGTECRSLPGDPRPRCVVPENAFSDP"
     gene            complement(1404..2399)
                     /locus_tag="VGB85_23335"
     CDS             complement(1404..2399)
                     /locus_tag="VGB85_23335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137044.1"
                     /translation="MTDSATPRPRPPGLREARLQAAISVLATGMLGLGLYLGNALTQA
                     PTTRQCTAETEDVDCDEGELCISGRCRAPRASGPLPCQEGDPCADCMCGEGYQCDAEQ
                     RCMPVKEDLCAPEVATLIRDIRRFEQDRCQAVGTDATQCDPKELDRFVIEHDKLNDLL
                     LQLQHTLTVHFDQNEPSAERGLPRPAQRYYGERFARLGDRLRSAKQILILGRSSRDRS
                     SSISRVIANNNLAQNRMQHVAQWIVDLAATPRDRDAMAKKLIRLSLGDRQPLTREQLA
                     KNPMHQYIAWKKGREDLLVSQVRGAEALDDSQREQVSRTLNQSVLVIPVPCEIPE"
     gene            complement(2396..3670)
                     /locus_tag="VGB85_23340"
     CDS             complement(2396..3670)
                     /locus_tag="VGB85_23340"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEY0137045.1"
                     /translation="MAAPSHLRLRADMAGSVSVWAFSLPGLVLSCLLTVLRLRSDHRC
                     DDTFMGACSGDGVLSCSTVLGDAWATVLGLPLTVYAAAFFVVTLALAGGTLVSPQVLR
                     PLVRPTLLVFAWAALVVCAALATYAAVGLHALCLYCIFIDGACLGVFLAALLANRGRL
                     LPRLPAPGQRGAAGLVFGVAALAFVTAVSVQRTLVLQYREGAAAAEALRPCVVALREL
                     EEPAIRIPSGLQADAAAPAFVAALFLDLACPHCKAEYEFWRGYQGELAARGVAVELQL
                     FHFPRGCSPEPGVADVSRARACDAARAHLCLGRGDSERSLAAFERLFSEQAEAFTSEH
                     LALVAAAFDVAADIGQADSPLFACMQGAETAAQLRRHLSFGETVAGLRAAPGALLIPL
                     VNGRPAGTAKQVQGRKPPEFYERWFRQQEAAR"
BASE COUNT          566 a         1486 c         1396 g          497 t
ORIGIN      
        1 ataaatgtcg agcgcctgga tcgcgtgcgc gtactggcgg gcgctgtacc gggccatgcc
       61 ggccttcttc cacagcccgg cgtccccgct ggtccgcgcg cactgctcga actgtcgcgc
      121 cgcctcgccg tacttgtccg cgctcgtccc tgcgatcgcc tcctgcgcgg ccggacacgc
      181 ggccttcgcc ggctcgctcg tcggggccgg cgggctcgcg ccggtggaag ctggcccgag
      241 ggacacgttc gcaagcacgg tcccatacaa atgtcccgac gcgctcacgc ccgcgggtgc
      301 tggccccggg aacatgttca ccaggacagg tccatacgcg cgtcccggcg ccgccgcggc
      361 cgccggcgcg agcaggctca gcgccacgac gcaggccagc gggaggttga gggggctcgt
      421 ccggaggttc gtgaggggag acatggagtg cgctaggggt cggagaacgc gttttcgggg
      481 acgacgcagc ggggtctcgg gtcgccgggc aggctccggc attcggtgcc gacgatgcag
      541 ccgcagtctg ccaggcaatt gccgcagtgc tcgccgccgt cgcaggtgcc gtcgccgcag
      601 ccgggcgggc tcggtagggc gctcggcggc ggctcgggcg gggcgcagcg cgggctgccg
      661 agctcgccgc gcagcgccga cacccgctcc gacagcgccg cagtgtcggc gaggtcgccg
      721 acgtggatcc accgcagttc attacgacag tcgagcaaca gcgtgatcgg gatgccctcg
      781 ccggtctcga gcagcccggc tccgcgcagc aggccctgga acgcgtcgcc gctggcgtcg
      841 atcagcggct cggccgaggt cgccggcatc tggtcgacaa gccggcgata tgcgtcaccg
      901 tcgttgaccg ccccgatgtg gatcggcaca aagcgaatgt cgcggcgcca gccctcgctc
      961 agcgcgcgga acatcccgat ctcccgcaca cacggcgcgc accaggtcgc ccagaggttc
     1021 acgaccttga ccgccggggt cccgcgcagc acctcgttga gggcgatcgt gccgatgatg
     1081 tcattggagc gcaggaaccc gagctgcagc ccgaacagcc cgggaagtcg gcgacccggc
     1141 gtcacctcgg cgacccggcg cacccccgcc ggcgccgcgc tcgggctggc cgccagcacc
     1201 tccggggtga tcgcgcgcgc ctgcgggctg gtcgtcagcg gcgcgaccgg gacgtgctgc
     1261 tcgatcaccg gcgcggcgac gatactccac accagcgcgc ccgcgagggc gaccaccgcc
     1321 gcgaccccac cgcgcagcac cagccgctgc tccgccggag tcagccgatc gacctcgtca
     1381 tcgccgtcga gatccgccat gccttactcc gggatctcgc agggcacggg gatgacgagc
     1441 accgactggt tgagcgtgcg cgagacctgc tcgcgctggc tgtcgtcgag cgcctcggcg
     1501 ccgcggacct gcgagaccag caggtcctcg cggcccttct tccaggcgat gtattgatgc
     1561 atcgggttct tcgccagctg ctcgcgggtc agcggctggc ggtcgccgag gctcaggcgg
     1621 atcagcttct tcgccatcgc gtcgcggtcg cgcggcgtcg ccgcgagatc gacgatccac
     1681 tgcgcgacgt gctgcatgcg gttctgcgcg aggttgttgt tggcgatcac ccgcgaaatg
     1741 gaagagctgc ggtcgcgact cgagcggccg aggatcagga tctgcttggc gctgcgcagg
     1801 cggtcgccga gccgggcgaa gcgctcgcca taatagcgct gggccgggcg cggcagcccg
     1861 cgctcggcgc tcggctcgtt ctgatcgaag tgcaccgtca aggtgtgttg tagctgcaac
     1921 agcaggtcgt tgagcttgtc gtgctcgatg acgaagcggt cgagctcctt cgggtcgcac
     1981 tgcgtcgcgt cggtgccgac cgcctggcag cggtcctgct cgaagcgacg aatgtcgcgg
     2041 atcagcgtcg cgacctccgg cgcacacagg tcctccttga ccggcatgca gcgctgctcc
     2101 gcgtcgcact gatagccctc gccgcacatg cagtcggcgc acgggtcacc ctcctggcac
     2161 ggcagcgggc cgctcgcgcg cggggcccgg cagcggcccg agatgcacag ctcgccctcg
     2221 tcgcagtcga cgtcctcggt ctccgcggtg cactggcggg tcgtcggcgc ctgcgtcagc
     2281 gcgttgccga ggtacagccc gagcccgagc atgccggtcg ccagcacgga gatcgctgcc
     2341 tgcaggcggg cctcgcggag ccccggcggg cgcgggcgtg gtgtcgcgct atcggtcacc
     2401 tcgccgcctc ctgctgacga aaccatcgct cgtagaactc cggcggcttg cgcccctgca
     2461 cctgcttggc cgtgcccgcc gggcggccgt tcaccagcgg gatcagcagc gcgcccgggg
     2521 ccgcgcgcag gccggcgacc gtctcgccga acgacaggtg acgccgcagc tgcgccgccg
     2581 tctcggcgcc ctgcatgcac gcgaacagcg gcgagtcggc ctgaccgatg tcggccgcga
     2641 catcgaaggc cgcggcgacc agggcgaggt gctcgctggt gaacgcctcc gcctgctccg
     2701 agaacagccg ctcgaacgcc gcgaggctgc gctcgctgtc gccgcggccg aggcacaggt
     2761 gcgcgcgcgc ggcgtcgcag gcgcgggccc ggctgacgtc ggcgaccccc ggctcggggc
     2821 tgcaaccgcg ggggaagtgg aacagttgca gctcgaccgc gacgccccgc gcggccagct
     2881 cgccctgata cccgcgccag aactcgtact cggccttgca gtgcgggcac gcgaggtcga
     2941 ggaacagcgc cgcgacgaac gccggcgcgg ccgcgtccgc ctgcaacccg ctcgggatgc
     3001 ggatcgcagg ctcctcgagc tcgcgcagcg cgacgacaca gggccgcagg gcctcggccg
     3061 cggccgcgcc ctcgcggtac tgcaacacca gcgtgcgctg caccgacacc gcggtcacga
     3121 aggccagcgc cgcgaccccg aacaccagcc cggccgcccc gcgctggccc ggcgccggca
     3181 gccgcggcag caggcgcccg cggttggcca gcagcgccgc caggaacacc ccgaggcacg
     3241 cgccgtcgat gaagatgcaa tacagacaca gcgcgtgcag gccgaccgcc gcgtaggtcg
     3301 ccagcgccgc gcagaccacc agcgccgccc aggcgaacac cagcagcgtc gggcgcacca
     3361 gcggccgcag cacctgcggg ctgaccagcg tgccccccgc gagcgcgagc gtgaccacga
     3421 agaaggccgc cgcgtagacc gtcagcggca gcccgagcac cgtcgcccac gcgtcgccga
     3481 gcaccgtgct gcacgacagc acgccgtcgc cggagcaggc gcccatgaag gtgtcgtcgc
     3541 agcgatggtc ggaccgcagg cgcagcaccg tcagcaggca gctgagcacc aggcccggca
     3601 ggctaaacgc ccacaccgac acgctccctg ccatgtcggc ccgcagccgc aggtgggagg
     3661 gtgcagccat gccgccgcga gcatagagcg ttcccgcacc gaacgcccag cgtaaagatc
     3721 gccccggaag catcgcgccg aggtcggccc gagcacgcgc actgttgccg ggcgcgaatg
     3781 acgctggagg gatgcccgac cccccgcgat cggccgagca tcacggcatg cgatccccgg
     3841 cgctgctgct caccctcgtg ctcgcctccg cctgcggcga cgacctcggc gccgacgatc
     3901 acgcgctcga cgacgacgcc ggcgacgacg cgcccgaccc cggcg
//
LOCUS       DASWNJ010001403         2691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_236304, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001403 DASWNJ010000000
VERSION     DASWNJ010001403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236304"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /locus_tag="VGB85_23345"
     CDS             <1..1321
                     /locus_tag="VGB85_23345"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0137046.1"
                     /translation="PPPKPDHVVVIVDRSRSTTPSMHREAIAAVGAVFDTLPAGLTFD
                     AISFARKARPLLPEGAAFPGVHDRAAREQVAAALDAGSREQGTDLAAALALAGKRIAA
                     RGAHRPLVLVITDGMLPLGAGPGPIGQVFADSLGPRKPAEKPELVFVVDEPMLTRTGI
                     HAEHPIAVLAAGLDARISLASLTTTADDTFARNLLRAPGVLRELDVSLPPQAVLEDSP
                     PTGLVAGNVVVLAGSYRRGPPGVKVRGKLGKTRPQLRPRPEVQVAPPAALVASIRPTG
                     PAQAAVEGFAVPPWYGRKYQRNAQLGITWAGRGRTTDRGHLDEKIFRRYLGTRVFPRA
                     RVCYNQALGRSQQLGGRVTFQFEVGKGEVMLATTDTTGLSERDPMFERCLLEAAWMLD
                     IPAGKLDDQIYRIRYPLVFNPPKTGRPALDEGVMGAGTVELLLNLKR"
     gene            1335..1916
                     /locus_tag="VGB85_23350"
     CDS             1335..1916
                     /locus_tag="VGB85_23350"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY0137047.1"
                     /translation="MRSALLLALAVLACGAPPANEGPAPATNEQATADDLAATLARIA
                     SAPEAVDRRPLMQQIHRAPAELALPALRAGLADPDPAIRTAAAHATGRRRDGKALASE
                     LIALATGDAVPAVRAAGCRGLGQLRLPGAFVPLQQNLSHETAEVRLAALRALARIDAA
                     RAAALPELGRLQLDPDARVAGAATKISRGVDLQ"
     gene            complement(1918..>2691)
                     /locus_tag="VGB85_23355"
     CDS             complement(1918..>2691)
                     /locus_tag="VGB85_23355"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY0137048.1"
                     /translation="FDPSSIVALVLAWGVFFAVTTVVLVVLGFAWERSALGRRRRIFA
                     LPVPAGQYRREALANVGFVILATAAFTAAIASGLLRADATGVVPAVVTFVVCFVGFEV
                     YYYVQHRLLHTRALVRFHRWHHASRLTTPLTGQSLGIVEALGWIGGLVLFPALLGGLG
                     LLHAGAMVTYLVMNATGNILGHANAEAHPRSSGTRANSWATHPFTFHALHHARWTGHY
                     GFGTTVLDRVLGSEWSDWPALHGRVADGEPLRSFKQRGE"
BASE COUNT          385 a         1136 c          865 g          305 t
ORIGIN      
        1 cccgcccccg aagcccgacc acgtcgtcgt catcgtcgac cggtcgcgca gcaccacccc
       61 gagcatgcac cgcgaggcga tcgcagccgt cggcgccgtg ttcgacacgc tgcccgccgg
      121 cctcaccttc gacgcgatca gcttcgcccg caaggcccgc ccgctgctgc ccgagggcgc
      181 cgcgttcccg ggcgtgcacg accgggccgc gcgcgagcag gtcgccgcgg cgctcgacgc
      241 cggctcgcgc gagcagggca ccgacctcgc cgccgccctc gccctcgccg gcaagcggat
      301 cgccgcgcgc ggggcccatc ggccgctcgt gctcgtcatc accgacggca tgctcccgct
      361 cggcgccggc ccgggcccga tcggccaggt cttcgccgac agcctcggac cgcgcaagcc
      421 cgccgagaag cccgagctcg tgttcgtcgt cgacgagccg atgctgacgc gcaccggcat
      481 ccacgccgag cacccgatcg ccgtgctcgc cgccggcctc gacgcgcgca tcagcctcgc
      541 cagcctgacc accaccgccg acgacacctt cgcccgcaac ctcctgcgcg cccccggcgt
      601 cctgcgcgag ctcgacgtct cgctgccgcc gcaagctgtc ctcgaggaca gcccgcccac
      661 cggcctggtc gccggcaacg tcgtcgtgct cgcgggcagc taccgccgcg gcccgccggg
      721 cgtaaaggtt cgcggcaagc tcggcaagac gcgcccgcag ctgcgcccgc gcccggaggt
      781 ccaggtcgcg ccgccggccg ccctcgtcgc gtcgatccgc ccgaccggac cggcgcaggc
      841 ggccgtcgag ggcttcgcgg tgccgccgtg gtacggccgc aagtaccagc gcaacgccca
      901 gctcggcatc acctgggccg gtcgcggccg caccaccgac cgcggccacc tcgacgagaa
      961 gatcttccgt cgctacctcg gcacccgcgt gttcccgcgg gcccgcgtct gttacaacca
     1021 ggcgctcggc cgctcgcagc agctcggcgg ccgcgtcacc ttccagttcg aggtcggcaa
     1081 gggcgaggtc atgctcgcca ccaccgacac caccggcctg agcgagcgcg acccgatgtt
     1141 cgagcgctgc ctgctcgagg ccgcgtggat gctcgacatc ccggccggca agctcgacga
     1201 ccagatctac cgcatccgct acccgctggt gttcaacccg ccgaagaccg gccggcccgc
     1261 gctcgacgag ggtgtcatgg gcgccggcac cgtcgagctg ctgctcaacc tcaagcgctg
     1321 acgatcgacc gatcatgcga tccgcgctgc tcctcgccct cgccgtgctc gcctgcggcg
     1381 cccccccggc gaacgagggg cccgcccccg cgaccaacga gcaggccacc gccgacgacc
     1441 tcgccgccac gctcgcgcgc atcgccagcg cgcccgaggc cgtcgaccgg cgcccgctga
     1501 tgcagcagat ccaccgcgcc ccggccgagc tcgcgcttcc ggccctgcgc gccggcctcg
     1561 cggaccccga ccctgcgatc cgcaccgcgg ccgcccacgc gaccggccgc cggcgcgacg
     1621 gcaaggccct cgccagcgag ctgatcgccc tcgccaccgg cgacgccgtg ccggccgtgc
     1681 gcgccgccgg ctgccgcggc ctcggccaac tgcgcctgcc cggcgcgttc gtcccgctgc
     1741 agcagaacct gtcccatgag acagccgagg tccgcctcgc cgccctgcgg gccctcgcgc
     1801 gcatcgacgc cgcccgggcc gccgccctgc ccgagctcgg ccgcctgcag ctcgaccccg
     1861 acgcgcgggt cgccggcgcc gcgaccaaga tctcacgcgg cgtcgacctg cagtgatcta
     1921 ctcgccgcgc tgcttgaacg accgcagcgg ctcaccatcg gcgacgcggc cgtgcagcgc
     1981 cggccagtcg ctccactcgc tgccgagcac gcggtcgagc accgtcgtgc cgaacccgta
     2041 gtgccccgtc cagcgcgcgt ggtgtagcgc atgaaacgtg aacgggtgcg tcgcccacga
     2101 gttggcgcgc gtgcccgacg accgcgggtg cgcctcggcg ttcgcgtgcc cgaggatgtt
     2161 gccggtcgcg ttcatcacca gataggtcac catcgccccg gcgtgcaaca agcccaggcc
     2221 gccgaggagc gccggaaaca gcaccagccc accgatccac cccagcgcct cgacgatgcc
     2281 gagcgactgt cctgtcagcg gcgtcgtcag ccgcgacgcg tgatgccagc gatgaaagcg
     2341 gaccagcgcg cgcgtgtgca acagccggtg ctgcacataa taatagacct cgaagccgac
     2401 gaagcagacc acgaaggtga cgaccgccgg gaccacgccg gtcgcgtcgg cccgcagcag
     2461 gccactggcg atcgccgccg tgaacgcagc ggtcgccagt atcacaaagc cgacgttcgc
     2521 cagcgcctcg cgccggtact gccccgccgg caccggcagc gcgaagatcc gccgcctccg
     2581 cccgagcgcg ctgcgctccc acgcgaagcc gaggacgacc agtacgaccg tcgtgaccgc
     2641 aaagaacacc ccccacgcga gcacgagcgc gacgatcgac gatggatcga a
//
LOCUS       DASWNJ010001404         1727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_661617, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001404 DASWNJ010000000
VERSION     DASWNJ010001404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661617"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VGB85_23360"
     CDS             <1..560
                     /locus_tag="VGB85_23360"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY0137049.1"
                     /translation="SSPQPVTDEVLFEGNAKHSANIGTYLMWVCVCIAGGAIGWGLLQ
                     IDALKDYPLWVLSLVGVPPLLWSYLVHIKRKYKISRRRIETETGVLSTRLDTLELWRV
                     LDVRYERSLIDQIFDNAKIILIGTDQTHATLVLHGMPGHRQMFERLRDAVQFARHSNR
                     PMELAPHHGGHEVDALTGGHDEHHH"
     gene            652..1032
                     /locus_tag="VGB85_23365"
     CDS             652..1032
                     /locus_tag="VGB85_23365"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEY0137050.1"
                     /translation="MTRPENPSALHILSFVYLTFSHVTDGVLAPEELETIARVLQGWL
                     PDAPPAVVQRVLVESAAWVNELGDDDARLTKAEEYAHLMRQQMNEKQRGAVLVNLIEV
                     ARADGKVTEREESFIARLTAILDG"
     gene            1400..1633
                     /locus_tag="VGB85_23370"
     CDS             1400..1633
                     /locus_tag="VGB85_23370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137051.1"
                     /translation="MIIHSPTPRPGAASSDHAEAANRDHRDAATADSSRSGEGDPFRQ
                     LFHRHANHVNSAQTRNIQARLRSRLFAEDRERG"
BASE COUNT          297 a          649 c          525 g          256 t
ORIGIN      
        1 gctcgagccc gcagccggtc accgacgagg tcctcttcga gggcaacgcc aagcacagcg
       61 ccaacatcgg cacctatctg atgtgggtct gcgtctgcat cgccggcggc gcgatcggct
      121 ggggcctgct gcagatcgac gccctcaagg actacccgct gtgggtcctg agcctcgtcg
      181 gcgtgccgcc gctgctgtgg agctacctcg tccacatcaa gcgcaagtac aagatcagcc
      241 gccgtcgcat cgagaccgag accggcgtgc tctcgacccg cctcgacacc ctcgagctgt
      301 ggcgcgtcct cgacgtccgc tacgagcgca gcctgatcga ccagatcttc gacaacgcga
      361 agatcatcct gatcggcacc gaccagaccc acgcgaccct cgtcctccac ggcatgcccg
      421 gccaccgcca gatgttcgag cgcctgcgcg acgccgtgca gttcgcccgc cacagcaacc
      481 gcccgatgga gctcgccccc caccacggcg gccacgaggt cgacgccctg accggcggcc
      541 acgacgagca ccaccactag accccgggct cgcgcgcgcg cctcgtcgcg cgtgcgcgcg
      601 agcggacacg ccccaaccca cgagccggcg cggggcatat catcggaggc gatgacccgc
      661 cccgagaacc cgtccgccct tcacattctc agcttcgtct acctgacctt tagccacgtc
      721 accgacggcg tgctcgcgcc cgaggagctc gagacgatcg cccgcgtgtt gcagggctgg
      781 ctgcccgacg ccccgccggc ggtggtccag cgcgtgctgg tcgagtcggc cgcgtgggtc
      841 aatgagctcg gcgacgacga cgcgcgactc accaaggccg aggagtatgc ccacctgatg
      901 cgccagcaga tgaacgagaa gcagcgcggc gcggtgctcg tcaacctcat cgaggtcgcg
      961 cgcgccgacg gcaaggtcac cgagcgcgag gagtcgttca tcgcgcgact gaccgcgatc
     1021 ctcgacggct gagacctgtc cgatcggaca gccgacccga ccgtcgcccg cgggctgcgg
     1081 tcgggttttt actgcgcctg aaaacacgtc atcggtttcg tgggtagtcc gggccacacc
     1141 cgcggcgatc gtgacagtgg acccctgcac aggccgacgt aggcggccac ggacagcgat
     1201 cggacctgct gccccaaagg acaggcgcgc cgccatcgac cgaaacatcc gagggaaaga
     1261 ccgtgtccgc gttcgtgcgg gctctccaca cgtggattcg tgggctcacg agatgtcgca
     1321 aagattttcg tgatccatcg accgactcga gacactacct ccacagcctg acgcatcccc
     1381 cgtccggaag gcccttttta tgatcatcca ttctccaact ccccgcccgg gagccgcctc
     1441 atccgaccac gccgaggcgg ccaaccgcga ccatcgtgac gctgcgaccg ccgatagcag
     1501 ccgttcgggc gagggcgacc cgtttcgcca gctgtttcac cgccacgcca accacgtgaa
     1561 cagcgcccag acgcgcaaca tccaggcccg cctgcgcagc cgtctgttcg ccgaggaccg
     1621 cgagcgcggc tgagctgctt cgtgtcacgg gctcgtgtca cgagctcgtg ccaccgcttc
     1681 gtgtcttcgt gtcaccggcg aggacccggc gaacacctct gtcgatg
//
LOCUS       DASWNJ010001405         3795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_484354, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001405 DASWNJ010000000
VERSION     DASWNJ010001405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484354"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VGB85_23375"
     CDS             complement(<1..477)
                     /locus_tag="VGB85_23375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEY0137052.1"
                     /translation="MKTAKLADGTEVYCLVRDEAVVLDHHVAGYLTNGIRIADGDVVF
                     DVGANIGVFGLRVVQRHANARVYAFEPVPPIFAVLAENAAHHGDGRLRAMPCGLSRAA
                     ATVSLTYFPHSPALSTAHPEMWDDDPGALQRAVKGSLQHPPPSLWWTRWVPGFMHGA"
     gene            complement(507..1883)
                     /locus_tag="VGB85_23380"
     CDS             complement(507..1883)
                     /locus_tag="VGB85_23380"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0137053.1"
                     /translation="MQPTSSDLTAPNAPRTPALVRGNWLLGVGPDLLRRPLALLDELS
                     ARGDVVGFRLPGMRAVLVNRPELIQRVLHDRRTFNKQMRSYRRVGALVGDGLATSDGD
                     FWLRQRRIAQPAFHRQRIAGFGATMVRFAEAAREQLGRAGGRPIDVFEAMNRLTLDIA
                     CEALLGPDAREDNEILGRTFTEVAAYLVDRLNLLWYLPLWVPTGPNRRCQAAVAALDA
                     AVHRIIARRRAQPHESLDLLSMMLHARDEDTGACMSDTQLRDEVVTMLLGGHETTSTA
                     LTWALHLLAGHPDVQARLHAELDAALGGRAPTVADLEQLPYTRMVVEETMRLYPPIWL
                     VARNAACDVDLGGFPIARGTAVFLSPWGAHRSPALWDAPEVFRPERFAPEHAAGRHRY
                     AYFPFVGGPRQCIGNAFALMEARLVLAVLLQRHYFTRAPGSVADPEPLLTLRPKAGLR
                     LLAHPRGP"
     gene            complement(2024..2638)
                     /locus_tag="VGB85_23385"
     CDS             complement(2024..2638)
                     /locus_tag="VGB85_23385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0137054.1"
                     /translation="MTRDEDLLAAWNSGDEGSGERLFDRYFVRIVRFFRSKVRGDIDE
                     LVQRTFLGCLEARGRFRGEGSFYAFLFGIARHVLVDHYRAQRRGAGVIDLEEVSLHDL
                     DPRPSSVLTERREHQILLESLARIPLESQLILELHYWEEMTSAAIADVLKVPHGTAQT
                     RIRRARQLLAAQIDRWAGSTSTSSFDFEPWARSLREQVDRSARR"
     gene            2759..>3795
                     /locus_tag="VGB85_23390"
     CDS             2759..>3795
                     /locus_tag="VGB85_23390"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0137055.1"
                     /translation="MEEAEDEALEMRHMRQELKRKLFRRAPEPLRLGRFVVLERLGEG
                     GMGVVFSGYDPTLDRKVAIKVLRSQPGELGDRQRLRTMREAQALARLSHANVIAVFEV
                     GELDGAVFIVMEFIRGVTLKDWLASTPRAPAEVLAVLVAAGRGLVAAHQAGLIHRDFK
                     PDNVMVGDDGSVRVLDFGLAALEASPAEAVTEASPDFDPASPASDRLRLTRTGVKVGT
                     PAYMAPEQWAGAPPDARGDQYSFCAVLHEALTGVRPNAGVTLAALERAESPPRPAPRS
                     ELPAWLRAAVQRGLARAPEQRFASLSQLIDLLARDPERRQRRQRAAQIVAAVMLTALI
                     ALGLVRGAAAL"
BASE COUNT          561 a         1240 c         1452 g          542 t
ORIGIN      
        1 cgcgccgtgc atgaagccgg ggacccaccg ggtccaccac agcgacggcg gcgggtgttg
       61 caggctgccc ttcaccgcgc gctgcagggc cccggggtcg tcgtcccaca tctccggatg
      121 cgccgtcgag agcgcgggcg agtggggaaa gtaggtcagc gagacggtcg ccgcggcgcg
      181 tgagagcccg cagggcatcg cccgcaggcg gccatcgccg tggtgcgccg cgttctcggc
      241 gagcaccgcg aagatcggcg gcactggttc aaaggcgtat acccgcgcgt tcgcgtggcg
      301 ctggaccacg cggagcccga agacgccgat gttggcgccg acgtcgaata cgacatcgcc
      361 gtcggcgatg cggatcccat tggtcaaata tccggcgacg tggtgatcga gcaccacggc
      421 ctcgtcccgg accaggcagt agacctcggt cccatccgcg agctttgctg tcttcatgaa
      481 aatgctttcg ggtggatcgt gggcggtcag ggaccgcggg ggtgcgcgag caggcgcagg
      541 ccggccttcg ggcgcagcgt cagcagcggc tcggggtcgg cgacgctgcc gggcgcgcgg
      601 gtgaagtagt gacgctggag cagcaccgcg agcacgagac gcgcctccat caatgcgaag
      661 gcgttgccga tgcactgccg cgggccgccg acgaacggga agtaggcgta gcggtggcgg
      721 cccgcggcgt gctccggggc gaagcgttcc gggcgaaaca cctccggcgc gtcccacagc
      781 gcgggcgagc ggtgcgcgcc ccaggggctg aggaacacgg cggtgccgcg ggcgatcggg
      841 aagccgccga ggtcgacgtc gcaggcggcg ttgcgggcga ccagccagat cggtggataa
      901 aggcgcatgg tctcctcgac gaccatgcgg gtgtagggca gctgctcgag gtcggcgacg
      961 gtgggagcgc ggccgccgag cgccgcgtcg agctccgcgt ggagtcgggc ctggacgtcg
     1021 gggtggccgg cgagcaggtg cagggcccag gtcagcgcgg tcgaggtggt ctcgtggccg
     1081 ccgagcagca tcgtcacgac ctcgtcgcgg agctgggtgt cgctcatgca cgcgccggtg
     1141 tcctcgtcgc gggcgtgcag catcatcgac agcaggtcga ggctctcgtg cggctgggcg
     1201 cggcggcggg cgatgatgcg atggacggcg gcgtcgaggg cggcgactgc cgcctggcag
     1261 cggcggttgg gtccggtcgg gacccacagc gggaggtacc agaggagatt gaggcggtcg
     1321 acgaggtagg cggcgacctc ggtgaaggtg cgaccgagga tctcgttgtc ctcgcgggca
     1381 tcggggccga gcagcgcctc gcaggcgatg tcgagggtga ggcgattcat cgcctcgaag
     1441 acgtcgatcg gccgtccgcc tgcgcgcccg agctgctcgc gtgctgcctc ggcgaagcgg
     1501 accatggtcg cgccgaagcc ggcgatgcgc tggcgatgaa acgccggctg ggcgatcctt
     1561 cgctggcgca gccagaagtc gccgtcgctg gtcgccaggc cgtcgccgac gagcgcgccg
     1621 acgcgacgat acgatcgcat ctgtttattg aaagtgcgcc tgtcgtgaag cacgcgctgg
     1681 atcagctcgg gccgattgac gagcaccgcg cgcatccccg gcaggcgaaa gccgacgacg
     1741 tccccccggg cgctcagctc gtccagcagg gccagcgggc ggcgcagcag gtccgggccg
     1801 acgccgagca gccagttgcc gcgtacgaga gccggcgtcc ggggagcgtt cggcgcggtg
     1861 aggtcggagc tggtcggttg catgggacaa ccgggggtcg cggctcgcca ggaattaatt
     1921 gcgactattt ttcgacgggt cgtgaaatcg aagacgcgac cgattaatct taaatgcctt
     1981 cgcggctcgc cgcgagcgat cgcggaggtc ttcgcgggtc tgctcagcgt cgggcgctgc
     2041 ggtcgacctg ctcgcggagc gagcgtgccc agggttcgaa gtcgaagctg ctggtgctgg
     2101 tcgagccggc ccagcgatcg atctgggcgg cgagcagctg gcgggccctg cggatgcggg
     2161 tctgcgccgt gccgtgcggg accttgagga cgtcggcgat cgcggccgag gtcatctcct
     2221 cccagtaatg cagctcgagg atcaactgcg attcgagggg gatccgggcg agcgactcga
     2281 ggaggatctg atgctcgcgg cgctcggtga gcacgctgct cgggcgcggg tcgaggtcgt
     2341 ggagcgagac ctcctcgagg tcgatcacac cggcgccgcg gcgctgcgcc cggtagtggt
     2401 cgacgagcac gtggcgggcg atgccgaaca aaaacgcgta gaagctgccc tcgccgcgaa
     2461 accggccgcg ggcctcgagg cagcccaaaa atgtccgctg gaccagctcg tcgatgtcgc
     2521 cgcggacctt gctacgaaag aagcggacga tccggacgaa gtatcggtcg aacagtcgct
     2581 ccccggaccc ctcgtcgccg ctgttccagg ccgcgagcag gtcctcatca cgcgtcatgg
     2641 tcgaagcgta cataccccgc cggggcagga caatcaccgt ttcgagggcg ttggcggcgc
     2701 aaccggcgcg agactgctga tgtggaacgg gtagactgac gtcgtattgt cgggagccat
     2761 ggaggaggcg gaagacgagg cgctggagat gcgccacatg cggcaggagc tcaagcgcaa
     2821 gctgtttcgc cgcgcgcccg agccgctgcg cctcggacgc ttcgtcgtgc tcgagcgcct
     2881 cggtgagggc ggcatgggcg tggtgttctc gggttacgac ccgaccctcg accgcaaggt
     2941 cgcgatcaag gtgctgcgca gccagccggg cgagctcggc gatcgccagc gcctgcgcac
     3001 gatgcgagag gcgcaggcgc tggccaggct gtcccacgcg aacgtgatcg ccgtgttcga
     3061 ggtcggcgag ctcgacggcg cggtgttcat cgtgatggaa ttcatccgcg gggtcacgct
     3121 caaggactgg ctcgcgagca caccacgcgc gccggccgag gtgctcgcgg tgctcgtcgc
     3181 ggccgggcgc gggctggtcg cggcgcacca ggccggcctg atccaccgcg acttcaagcc
     3241 cgacaacgtg atggtcggcg acgacggcag cgtgcgcgtc ctcgacttcg ggctcgcggc
     3301 gctcgaggcc tcgcccgccg aggcggtgac cgaagcctcg ccggacttcg accccgcctc
     3361 gccggccagc gaccgactcc gcctgacccg caccggcgtc aaggtcggca cgccggctta
     3421 catggcgccc gagcagtggg cgggcgcacc tccggacgcg cgcggtgacc agtactcgtt
     3481 ctgcgcggtg cttcacgagg cgctgaccgg cgttcgtccc aacgcgggcg tgacgctggc
     3541 cgcgctcgag cgcgccgaga gtccgccgcg tccggcaccg cgatccgagc tgccggcgtg
     3601 gttgcgcgct gccgtgcaac gcgggctcgc gcgcgcgccg gagcagcgct tcgcgtcgct
     3661 gtcgcagctg atcgacctgc tcgcgcgcga cccggagcga cgccagcgac gccagcgtgc
     3721 ggcgcagatc gtcgccgcgg tgatgctgac cgccctgatc gcgctcggcc tcgtgcgtgg
     3781 tgcggccgcg ctgca
//
LOCUS       DASWNJ010001406         3352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_95131, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001406 DASWNJ010000000
VERSION     DASWNJ010001406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95131"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VGB85_23395"
     CDS             complement(<1..304)
                     /locus_tag="VGB85_23395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137056.1"
                     /translation="MIAPLDLWHRISLAGVGPDMEDSFRRKIVLTNQISALAGAAVLV
                     ISLVGRIAVAGQTVALVLLAFGVAGYFSIPLLNHARRHELARVVLTLLPPIHTILDT"
     gene            complement(474..2051)
                     /locus_tag="VGB85_23400"
     CDS             complement(474..2051)
                     /locus_tag="VGB85_23400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263577174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HEY0137057.1"
                     /translation="MKRLGCGAMLWALAACPAPGGSTTAEEDPSSGSESSGAESSEPT
                     TATGEEEPVALHGAVQKGPFILGTSVSVAPLNAMGEPTGQQFEAPTSNDVGEFSVAGV
                     PAGPVALLASGYHFDEVRGGLSTAPLTLRALHQAGQGSTTVNLHVLGHLVEPRVRGLV
                     GGGMAVADALAIAEAEAVAALGVGKDGFALTVPAAQASVVGADNDDNAYVFALSAVIA
                     QAAHLAGPDAPDAALQALLNELALDLSDDGAITPAHRDELAAAETALQAAQVRAGLAD
                     YLAGLGSPPPPDLERIVDQDHDGLANSDDNCPFVGNVPQADQDGDGKGDACDDCPQSG
                     VDMDGDGYDDGCDNCPAVPNADPPQDAMPQLGILSDTDHDGLGNACDACPRSAATGAV
                     PGENCCDPRSSVCVKDPGSTLSWDCLPTDGGLRFDCVEVFQTCPTYQTCFGCDGPCVG
                     PGGLANECKGASSCDCTSYSCGVQWCTLGGDCPYGNTCITWFKPGEAPAGLEQLGICV
                     IADSGPCAGKVGRECAG"
     gene            2444..>3352
                     /locus_tag="VGB85_23405"
     CDS             2444..>3352
                     /locus_tag="VGB85_23405"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEY0137058.1"
                     /translation="MTDIIASFRDPDAARVWNYWFAHRDRSGAAQAVSDPFADDYVLG
                     QALWFRGGPEVDAEIAAHFEPLLRRAAAGELDAWRETPRECLALLLLLDQFPRNMYRG
                     TGQAFAYDAIALDLARDAIARGLDRPLAPAEALFFHMALTHSEQLADVRAALAGIREL
                     ATRCTRGQQRLARSWQVGTQKHIDVLERFGRYPHRNHALGRASTPAEEAFLQHPEFTA
                     LFMRSQLPRAVDPAQEAVARAAAPARRLKILALHGFRQNGEVFRARIRKLRDALADLA
                     DLVFVTSPIAYTPQGDARDGTLAAFGE"
BASE COUNT          470 a         1262 c         1151 g          469 t
ORIGIN      
        1 ccgtgtcgag gatggtgtgg atcggcggca gcagtgtcaa aacgacgcgg gcgagctcgt
       61 ggcgtcgtgc atggttgagc agagggatcg agaagtatcc ggcgaccccg aacgccagca
      121 atacgagcgc gaccgtctga cccgcgacgg cgatccggcc cacgagggag atcacgagga
      181 cggcggcgcc agcgagggcg ctgatctggt tcgtcaggac gatcttgcgg cggaaggagt
      241 cctccatgtc cgggccgaca ccagcgagcg agatccgatg ccagaggtcg agcggcgcga
      301 tcatcggcgc catcgtaccg catgagcgcg ggggggacgg cggcgtgtga gctggtggcg
      361 cacgccggca tcccggaacc gcgagctcga gtacacgccg cgacggtctc aaaagctctg
      421 cggggccacg actcctgctc tcgcggcgca gctggcccgg gggacaggtc gcctcaccct
      481 gcgcattcgc ggccgacttt acccgcgcac ggccccgagt ccgcgatcac gcagatcccg
      541 agctgctcga gccccgcggg cgcctcgccc ggcttgaacc acgtgatgca cgtgttgccg
      601 tacgggcaat ccccgccgag cgtgcaccac tgcacgccgc agctatagct cgtgcagtcg
      661 cacgagctgg cgcccttgca ctcgttggcg aggccgccgg gcccgacgca cggcccgtcg
      721 cagccgaagc aggtctgata ggtcgggcag gtctggaaca cttcgacgca gtcgaagcgc
      781 aggccgccgt cggtcggcag gcagtcccag gacagcgtcg agcccgggtc cttgacgcac
      841 accgaggacc gcgggtcgca gcagttctcg ccggggaccg ccccggtggc ggcggaccgc
      901 gggcacgcgt cgcatgcatt gccgagcccg tcatggtcgg tgtcgctgag gatcccgagc
      961 tgcggcatgg cgtcctgcgg cgggtcggcg ttgggcaccg ccgggcaatt gtcgcagccg
     1021 tcgtcgtagc cgtcgccgtc catgtcgacg ccgctctgcg ggcagtcgtc gcaggcgtcg
     1081 cccttgccgt cgccgtcctg gtcggcctgc gggacgttcc cgacgaacgg gcagttatca
     1141 tcgctgttgg cgaggccgtc gtggtcctga tcgacgatcc gctcgaggtc cggtggcggc
     1201 ggcgacccga gcccggcgag gtagtccgcg agcccggccc gaacctgcgc ggcctgcagc
     1261 gcggtctcgg ccgccgcgag ctcgtccctg tgcgccggcg tgatcgcgcc gtcgtcggag
     1321 aggtcgagcg ccagctcgtt gagcaacgcc tgcagcgccg cgtcgggcgc gtcgggcccc
     1381 gcgaggtggg ccgcctgcgc gatcacggcg ctgagcgcga agacgtacgc gttgtcgtcg
     1441 ttgtcggccc cgaccacgct cgcctgcgcc gccgggaccg tgagcgcgaa gccgtccttg
     1501 ccgacgccga gcgccgcgac ggcctccgcc tccgcgatcg ccagcgcgtc cgcgaccgcc
     1561 atcccgccgc cgaccaggcc gcgcacgcgc ggctcgacca ggtggccgag cacgtgcaga
     1621 ttgacggtcg tcgagccctg acccgcctgg tgtagcgcac gcagggtcag cggcgcggtc
     1681 gacagcccgc cgcggacctc gtcgaagtga tacccgctcg ccagcagcgc gaccggcccg
     1741 gccgggaccc cggcgacgct gaactcgccg acgtcgttgc tggtcggcgc ctcgaactgc
     1801 tgccccgtcg gctcgcccat ggcgttgagc ggtgccaccg acaccgaggt cccgaggatg
     1861 aacggcccct tttgcaccgc cccgtgcagc gccacgggct cctcctcgcc ggtcgccgtc
     1921 gtcggctcgc tcgactcggc cccgctcgac tcggacccgc tcgacgggtc ctcctcggcc
     1981 gtcgtcgacc ctcccggtgc cggacacgcc gccagcgccc acaacatcgc accgcacccg
     2041 agtcgcttca agtcaggagt catcattgga tggcgtccac gatcccaccg atggatcacg
     2101 gccgccactc aattccgacc tcgccagcgc aactcggggc gagccacccg aggccgacga
     2161 caccgtgcat ggagaaaata gacgccggtt ctctggtgat atgctccggc tcctcggcgg
     2221 cgagcaaatg caccgtcgct gtgatgccaa tggcaacgca agatcgcggc gacgtggcat
     2281 ccgaagtccg ccacgctcgg tgcgagcgcg cccggattag ccccgccgcc gccgcgcgat
     2341 cacgtcggct tgtcgcgccg tgagccacct gatagcttgc ccggcccgtc gtggcgtgat
     2401 cgagggctat cgtcgctcgc ggccggcgga tctgcgaggg acagtgacag acatcatcgc
     2461 cagctttcgc gaccccgacg cggcccgcgt ctggaactac tggttcgccc accgtgaccg
     2521 cagcggcgcg gcgcaggccg tcagcgaccc gttcgccgac gactacgtac tcggccaggc
     2581 gctgtggttt cgtggcggcc ccgaggtcga cgccgagatc gcggcgcact tcgagccgct
     2641 gctgcggcgc gccgcggcgg gtgagctcga cgcctggcgc gagaccccgc gcgagtgcct
     2701 cgcactgctg ctgttgctag atcagttccc gcgcaacatg taccgcggca ccggccaggc
     2761 cttcgcatac gacgcgatcg ccctcgacct cgcccgcgac gcgatcgcgc gcggcctcga
     2821 ccgaccgctc gcccccgccg aggccctgtt cttccacatg gccctgacgc actcggagca
     2881 gctcgccgac gtgcgcgcgg cgctggccgg catccgcgag ctggcgaccc gctgcacgcg
     2941 cggccagcag cgcctggccc gttcgtggca ggtcggcacc cagaagcaca tcgacgtgct
     3001 cgagcgcttc ggccgctatc cgcaccgcaa ccacgccctc ggccgcgcga gcacgccggc
     3061 cgaggaggcg ttcctgcagc acccggagtt caccgcgctg ttcatgcgct cgcagctgcc
     3121 gcgcgccgtc gaccccgcgc aggaagccgt cgcgcgagcc gcagcgcccg cgcgacggct
     3181 gaagatcctc gcgttgcatg gatttcgcca gaacggcgag gtgttccgcg cgcgcatccg
     3241 caagctgcgc gacgccctcg ccgacctcgc cgacctcgtg ttcgtcacct cgccgatcgc
     3301 ctacacgccg cagggcgacg cccgcgacgg caccctcgcc gcgttcggcg ag
//
LOCUS       DASWNJ010001407         5179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_484428, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001407 DASWNJ010000000
VERSION     DASWNJ010001407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484428"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /gene="allB"
                     /locus_tag="VGB85_23410"
     CDS             <1..935
                     /gene="allB"
                     /locus_tag="VGB85_23410"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR03178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HEY0137059.1"
                     /translation="GFKCFLTPSGVDEFPYVQEHELHPAMRELARIRSTLLVHAEMPG
                     PIEAAEAELARGNADPRNYMTYLASRPRASENDAIALLVRLCGDTGARTHVVHHASSD
                     ALPMIAGARASGLPFSAETCPHYLSFAAEDIPDGATAFKCAPPIRERDNRELLWQALR
                     DGVLDLVASDHSPCTPALKRQEAGDFQNAWGGISGLQLALSVVWTEARARGFAIEDIV
                     RWMCAAPARLAGISNRKGRLAPGADADIVIFHDDATHTVEPSRVLHRHKVTPYAGRTL
                     YGVVRSTYLRGQRVWHEGALEGTTQGRTFARERT"
     gene            932..2443
                     /gene="alc"
                     /locus_tag="VGB85_23415"
     CDS             932..2443
                     /gene="alc"
                     /locus_tag="VGB85_23415"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HEY0137060.1"
                     /translation="MSEPNSTSEPAEFRGLIDLAAAAYGGVAILANDEFFAEKDNLLR
                     AHAAVWKEHEYTDRGKWMDGWETRRRREPGYDWCIVRLGLPGVIRGVVVDTAYFRGNF
                     PAECSIEACTLTGFADLTTSIDSATEWTEILPRSPLRGHTQNEFAIAADARFTHVRLN
                     IFPDGGVARLRVHGEVRPDWKTLTRHGGLVDLAAVEHGGWSEACSDMFFGARQNLIGP
                     GRPFNMSDGWETRRRRGPGHDWNIVRLGAPGIIRRLEIDTTHFRGNAPGRVMVEAQGE
                     DGEWRVLLPETKTQPHTRHFFEEALRVLGPVNRVRLNVYPCGGVARLRAWAEPVLAAH
                     GALDRLNALPEPEARAALLACCGAPRWAEKMARARPFEDLGALLRLADRTWWSLGEDD
                     RLAAFAAHPRIGGKKAEVDTGARAASWSAGEQSGVDGAASTVRERLAAANDTYAERLG
                     FTFIVCATGKSADEMLALLEQRLQNSREIELRTAAEEQAAITRLRLIKMVEDR"
     gene            2443..2769
                     /gene="uraH"
                     /locus_tag="VGB85_23420"
     CDS             2443..2769
                     /gene="uraH"
                     /locus_tag="VGB85_23420"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEY0137061.1"
                     /translation="MGISTHILDTATGRPAPAVPIRLEFRQPDGSWREVGAGATDDDG
                     RLKTLTPAGPAPHGIYRIHFDTRAYLGADAFFPAVQIEFVVRDDRHHHVPLLLSPFGY
                     STYRGS"
     gene            3011..3742
                     /locus_tag="VGB85_23425"
     CDS             3011..3742
                     /locus_tag="VGB85_23425"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEY0137062.1"
                     /translation="MDGRTVDAKGFRLRGREVSRLEALSDAVFGFAITLLVVSLEVPR
                     TAAALFETMNGFVGFAACFALLFLIWHNQNRFFRRYGLNDGWTVALNGVLLFVIVFFV
                     YPLKFVFTLVIDMMRGARPLLQTHDGVPVLTTDQWPQLMMIYGAGYMAVFMIFALLHL
                     NALRLADVIELSELERHDTIDNVYESLLNVGIGAASVLLAVFGGEMGPRLSGLVYWLV
                     GPVLLVHGWISGRARKQIQTRVATG"
     gene            complement(4104..4865)
                     /locus_tag="VGB85_23430"
     CDS             complement(4104..4865)
                     /locus_tag="VGB85_23430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137063.1"
                     /translation="MSSPAPALTIALYSEDRTDADSNFAVLREIVYGMLQQVASDLKT
                     NHVKLEPAQPVRTERVSGSYWKVTGRAEAGAQERRRRLIRDVAAALRLGRVVVFHVDA
                     DEVWSRRGQCANVHEHWPRFTRDVRTTLHHAGYPVDDSALAQTLIEAIPFSEIESWAF
                     ANTARLRALLSEPRDLALLKSWEEDLAVLDEIPDIKDVLTIRDAHNQDLVQRRNGFPA
                     AALVAANKSYAAVVRQLRASAVVSRGVADAAGRPF"
     gene            complement(4862..>5179)
                     /locus_tag="VGB85_23435"
     CDS             complement(4862..>5179)
                     /locus_tag="VGB85_23435"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0137064.1"
                     /translation="LLSHPDSPILIDEVDNGLHPRLVETLLQLLAGRQSFLASHNKIV
                     IDYTNFDGPEDVRRKIHIVQRAKDGRQSVLVLDDETARAVYDKIEVAIQSPSDVLLAE
                     GLW"
BASE COUNT          813 a         1806 c         1736 g          824 t
ORIGIN      
        1 tcggcttcaa gtgttttttg acgccctcgg gcgtcgacga gttcccgtac gtgcaggagc
       61 acgagctgca ccccgcgatg cgcgagctgg cgaggatccg ctcgaccttg ctggtccacg
      121 ccgagatgcc cggcccgatc gaggccgccg aggccgagct ggcgcgcggc aacgccgacc
      181 cgcgcaacta catgacatac ctcgcctcgc ggccgcgggc ctccgagaac gacgcgatcg
      241 ccctgctcgt gcgcctgtgc ggcgacaccg gggcgcgcac ccacgtcgtg caccacgcct
      301 cctccgatgc attgcccatg atcgccggcg cgcgcgcgtc cggcctgcca ttttcggccg
      361 agacttgccc gcattacctc agcttcgccg ccgaggacat cccggacggc gcgaccgcct
      421 tcaagtgcgc cccgccgatc cgcgagcgcg acaaccgtga gctgctgtgg caggccctgc
      481 gcgacggggt gctcgacctc gtcgcgtccg atcattcgcc ctgcaccccc gcgctcaagc
      541 ggcaagaggc cggtgatttt caaaatgcgt ggggcggcat ctcgggcttg cagctggcgc
      601 tgtcggtcgt gtggaccgag gcgcgcgcgc gcggctttgc gatcgaagac atcgtgcgct
      661 ggatgtgtgc cgccccggcc cggctcgcag gtatcagcaa tcgcaagggt cgcctcgcgc
      721 ccggggccga cgccgacatc gtcatttttc atgatgacgc gacgcacacc gtcgagccca
      781 gtcgggtgct acaccgccac aaggtgaccc cttacgccgg acgcacgcta tacggcgtcg
      841 tgcgatccac ctacctgcgg ggccagcgtg tctggcacga gggcgccctc gagggcacca
      901 cgcagggtcg aaccttcgcc cgggagcgga catgagcgaa ccaaattcaa ccagcgaacc
      961 cgcggagttc cgcggcctga tcgacctcgc ggccgccgcc tacggcggtg tggccatttt
     1021 ggcgaacgac gagttcttcg ccgaaaaaga caacctgctg cgcgcccacg cggccgtgtg
     1081 gaaggagcac gaatacaccg accgcggcaa atggatggac ggctgggaga cccgccgccg
     1141 ccgcgagccc ggttatgact ggtgcatcgt ccgcctcggc ctgccgggtg tgatccgcgg
     1201 cgtggtcgtc gataccgcgt actttagagg gaacttcccc gccgagtgct cgatcgaggc
     1261 atgcacgctg accggcttcg ccgacctgac cacgagcatc gactccgcca ccgagtggac
     1321 ggagatcctg ccgcgctcgc cgctgcgcgg ccacacgcag aacgagttcg cgatcgcagc
     1381 ggacgcccgc ttcacgcacg tgcgcctcaa catttttcca gatggcggcg tcgcccgcct
     1441 gcgcgtgcac ggcgaggtgc gcccggactg gaagacattg acgcgccacg gcggcctcgt
     1501 cgacctcgcg gcggtcgagc acggcggctg gtcggaggcg tgcagcgaca tgttcttcgg
     1561 cgcgcgccag aacctgatcg gtcccgggcg ccccttcaac atgtccgacg gctgggagac
     1621 gcgccgccgt cgcggccccg gccacgactg gaacatcgtg cggctcggcg ccccgggtat
     1681 cattcgtcgc ctcgagatcg acaccaccca ctttcgtggc aacgcccccg gacgcgtgat
     1741 ggtcgaggcc cagggtgagg acggcgagtg gcgcgtgctg ctgccggaga cgaagacgca
     1801 gccacacacg cgacacttct ttgaggaggc gttgcgcgtg ctcggaccgg tgaaccgcgt
     1861 gcgcctcaac gtgtacccct gcggcggcgt cgctcgcctg cgcgcgtggg ccgagccggt
     1921 gctcgccgct cacggcgcgc tcgaccgcct caacgccctg cccgagccgg aggcccgcgc
     1981 cgcgctgctc gcctgctgcg gcgcgccgcg ctgggccgag aagatggccc gcgcgcggcc
     2041 cttcgaggac ctcggcgccc tgctgcgcct cgcggaccgc acgtggtggt cgctcggcga
     2101 ggacgaccgc ctcgcggcct tcgccgcgca cccgcggatc ggcggcaaga aggccgaggt
     2161 cgacaccggc gcgcgcgcgg cctcgtggtc ggccggcgag cagtcggggg tcgatggcgc
     2221 cgcgagcacg gtgcgtgagc gcctcgccgc cgccaacgac acctacgccg agcgcctcgg
     2281 cttcaccttc atcgtctgcg cgaccggcaa gtccgccgac gagatgctcg cgctgctcga
     2341 gcaacgcctg caaaactccc gcgagatcga gctacgcacc gccgccgagg agcaagccgc
     2401 gatcacgcgc ctgcggctta ttaaaatggt ggaggatcgc tgatggggat ctcgacgcac
     2461 atcctcgaca ccgcgaccgg ccggccggcg cccgccgtcc cgatccggct cgagtttcgt
     2521 cagcccgacg gcagctggcg ggaggtcggc gccggtgcga ccgacgacga cgggcgcctc
     2581 aagacgctga cgcccgccgg ccccgcgccg cacggtatct atcgcatcca cttcgacacc
     2641 cgcgcctatc tcggcgcgga cgcgttcttc cccgcggtgc agatcgaatt cgtcgtccgc
     2701 gacgaccgcc atcaccacgt gccgctgctg ctctcgccct tcggctactc gacctaccgc
     2761 gggagctgaa aagcgcgcgt gcggcctgca cggcccgcca caggtctctg cgagcatcgc
     2821 tgcgacgatg ctcgccaggc catgacgacc cgcctctggg cgctgcggcg cgccatgaat
     2881 gcgtatgaca cgcgagcgtg gcgccgttat gctggcggcc gggacggccg tcaggtcctt
     2941 gccaccttgc gcgacgcgaa gcgtacgcga gatacaaccc ccaggaggcg tgatgattca
     3001 gtccgggacg atggatggcc gcaccgtgga tgcgaagggc tttcgcctgc gcgggcgcga
     3061 ggtgtcgcgg ctggaggcgc tgagcgacgc cgtgttcggc ttcgccatca ccctgctggt
     3121 ggtttcgctg gaggtgccgc gcaccgccgc ggcgctcttc gagacgatga acggcttcgt
     3181 ggggttcgcc gcgtgctttg cgctgctgtt cctcatctgg cataaccaga accgcttctt
     3241 tcgccgctac gggctcaacg acgggtggac ggtggcgcta aacggcgtgc tgctgttcgt
     3301 catcgtgttc ttcgtctatc cgctcaagtt cgtgttcacc ctggtgatcg acatgatgcg
     3361 cggcgcccgg ccgttgcttc agacgcatga cggggtgccg gtgctcacca cggaccagtg
     3421 gccgcagtta atgatgatat acggcgcggg atacatggcc gtgttcatga tcttcgcgct
     3481 gctgcacctc aacgcgctac ggctggcgga cgtaattgag ctgtcggagc tggagcgcca
     3541 cgacacgatc gacaacgtgt acgaaagcct gctcaacgtg gggatcggcg cggcgtcggt
     3601 gttgctggcg gtgttcggcg gtgagatggg gccccggttg agcgggctgg tgtattggct
     3661 ggtgggcccc gtgctgctgg ttcatggctg gatcagcggc cgcgcccgca agcagatcca
     3721 gacgcgcgtc gccaccggct aaagccggcg gcgagatcgt ggtcgcgcgc cacccgaggc
     3781 gcgaccggga agggccttac cggggtgacc gctccggacg ccggtcggag gtcagcgtgc
     3841 cgcgccgagg gcgagctcga cgagccgcca cagacctgtc gcttttgcga cgcgccgcgc
     3901 ggtgaaaagg cgaagcagcc ggaagttcgc cgactacgcg gatagcgggc cgggcctccg
     3961 gtgcgacgcc ggggcccggg cggcgacctg acgcccggcg ctgccggaca cgctctgcac
     4021 tccgcagcgt cgctcggatc caaggactcg ctgtgcagtt cgcccggaag aatgagcggg
     4081 ccgcatgccg catcgggtag cgactaaaat ggccgcccgg ccgcgtcggc gaccccgcgg
     4141 ctgaccacgg ctgaagcgcg aagctggcgc acaacagctg cgtaggactt attggcggcg
     4201 accaacgcag ccgcggggaa gccgttccgg cgttgcacga ggtcttgatt gtgcgcatca
     4261 cggatagtca agacatcctt gatatccggg atctcgtcga gcactgccaa gtcctcctcc
     4321 cagctcttca gcaacgccag atcccgcggc tccgacagga gcgcccgcaa tcgggcagtg
     4381 ttggcgaagg cccagctctc gatctccgag aatgggatcg cctcgatcag cgtctgcgcg
     4441 agcgccgagt catcaaccgg gtatccggca tgatgaagcg tcgtccggac gtcacgggta
     4501 aagcgcggcc agtgctcgtg tacgttcgca cactggccac gacgcgacca tacctcgtca
     4561 gcgtcgacgt gaaacacgac gacacgaccg agccgcagcg cagccgcgac gtctcgaata
     4621 agccgccgtc gccgctcctg cgctcccgct tcggcccggc cggtcacttt ccagtatgaa
     4681 ccactgaccc gctccgtgcg cacgggttgg gcgggctcca gcttcacatg attggtcttg
     4741 agatccgatg ctacctgctg aagcatcccg tacacgatct cccggagcac cgcgaagttg
     4801 ctgtcggcgt cggtccggtc ctcggaatag agcgcgatcg tcagcgccgg ggccggtgag
     4861 ctcaccacag gccctccgcg aggagcacat cgctcgggct ctggatcgcc acctcgatct
     4921 tgtcgtaaac cgcgcgagcg gtctcgtcat cgagcaccag cacgctctgc cgaccatcct
     4981 tcgcgcgctg gacgatgtgg atctttcgcc gaacgtcctc gggtccgtcg aaattcgtat
     5041 agtcgatgac gatcttgttg tgactggcga gaaacgactg ccgaccggcg aggagctgca
     5101 acagggtctc gaccagacgc gggtggagac cattgtcgac ctcgtcgatc aagatcggcg
     5161 aatccggatg agagagcag
//
LOCUS       DASWNJ010001408         3367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_95141, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001408 DASWNJ010000000
VERSION     DASWNJ010001408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95141"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..880)
                     /locus_tag="VGB85_23440"
     CDS             complement(107..880)
                     /locus_tag="VGB85_23440"
                     /inference="COORDINATES: protein motif:HMM:NF014000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HEY0137065.1"
                     /translation="MRASIQAAGCKLLYRQWVGTVQQDLSFKFDPYLPLIDPEHFTDI
                     AFYRELEKIGYRQVLLGGTGVSNLAEVVTQIKRQTDLQVVLYPAGPDSLADADVVLLP
                     DVMNTNSHHGRPFGSGAVSTAVNIARRGLNFLPLAYFIMGESTARWYYDAFLIKKPKV
                     ILGYATYARMVGYRFLALDYEDPELNIDPELITALRTIEGMHLVVSDEFTPASAVEAR
                     ALGIDTVVTPSNLYEESSDPLAMAADFHRALLRPGAGTA"
     gene            complement(1043..1423)
                     /locus_tag="VGB85_23445"
     CDS             complement(1043..1423)
                     /locus_tag="VGB85_23445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137066.1"
                     /translation="MKRLYSLFMLPLLALTACGPRYDGVVLELYSAPPVPVRVNGEEI
                     EVAAGVAAAVDVRPLSSNQFDYYESDQLVLTSQDRDILRVEPTEAPRRFVIIGVAPGE
                     TCVDIEVDYEDHGCIPATVIAATP"
     gene            complement(1420..2259)
                     /locus_tag="VGB85_23450"
     CDS             complement(1420..2259)
                     /locus_tag="VGB85_23450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137067.1"
                     /translation="MRKTSYVLALGLTACSDTYDPNPGPKYGELGVGVFLYECPSSGD
                     PFCSDGTGTGDQFPQAFALGGRINLEYDWRDDAEHIGDPPPQLQSAAPSLLTRKGDGF
                     TALATGYAAVLAVTGNNEIVDLVHLHIREIAELQVVPADQHGAAPLQQLTLDRGGERE
                     LQAIAVDSDSVLLGGVLGHTWTSADPTIVSIVAGGDSGRARIEALQSGTTTLTLAHDA
                     MSVQLTITVEGEDDETGSESGSESSSSGDSNGDASSDSSGESSGSSGDTGGDSSGSSG
                     GVL"
     gene            2582..>3367
                     /locus_tag="VGB85_23455"
     CDS             2582..>3367
                     /locus_tag="VGB85_23455"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY0137068.1"
                     /translation="MEALFDASLGTGTLLLLILSLLIAFGFEFVNGFHDTANAVATVI
                     YTRSLRPWTAVILSGVCNFLGVFIGGIAVAMSIISLLPVELLVASGTGAGLAMLLALL
                     VAAIVWNLGTWYLGLPASSSHTLIGAIVGVGLANSLLPGHQFGAGVNWAKAGDVGLAL
                     LVSPLVGFTVAALLLLAAKKLIRDPQLYRPPVGTQPPPRWIRAVLIGTCTGVSFAHGS
                     NDGQKGVGLVMLVLIGIVPAGFALNVGISAPDLARATRALDTVE"
BASE COUNT          477 a         1088 c         1258 g          544 t
ORIGIN      
        1 atctggaagg ggcggcccgg cgggggcagc gggaccgccg gggcgtgggc gggtagcggc
       61 cggggcagca ggcgcaggcg cccggccgcg gtgctctggt cgctggtcac gcggtcccgg
      121 cccctggacg gagcagggcg cgatggaagt ccgccgccat cgccagcggg tcgctcgact
      181 cctcgtagag gttcgagggc gtgacgacgg tgtcgatgcc gagcgcgcgg gcctcgacgg
      241 cggaggccgg cgtgaactcg tcggagacga cgaggtgcat gccctcgatc gtgcgcagcg
      301 cggtgatcag ctcggggtcg atgttgagct cggggtcctc gtagtcgagc gcgaggaagc
      361 ggtagccgac catgcgggcg taggtcgcgt agccgaggat caccttgggc tttttgatca
      421 ggaaggcgtc gtagtaccag cgcgccgtcg actcacccat gatgaagtac gcgagcggca
      481 ggaagttgag gccgcgccgg gcgatgttca ccgccgtcga caccgcgccg gacccgaacg
      541 gccggccgtg atgcgagttg gtgttcatga catcgggcag cagcacgacg tcggcgtccg
      601 cgagcgagtc gggccccgcg gggtagagga cgacctgcag gtcggtctgc cgcttgatct
      661 gcgtgacgac ctcggcgagg ttgctgaccc cggtgccgcc gagcagcacc tgccggtagc
      721 cgatcttctc cagctcgcga taaaacgcga tgtccgtgaa gtgctcggga tcgatcagcg
      781 gcaggtacgg atcgaacttg aagctgaggt cttgctgcac ggtccccacc cactggcgat
      841 agaggagctt acaccctgcc gcctggatcg acgcccgcat gtcccttcgt tcacgtcgag
      901 tgccagcatc tcccgtggca gaggccatcg cgcgagcacg aggaccgacg cgccggatcg
      961 ctcatgcgat cggcgcggtc tgcgtcggcc gaaggtgcca gaggacggag cgagcgccgg
     1021 tcgcagcgat cgcagcgagc gatcagggtg tggcggcgat caccgtcgcg gggatgcagc
     1081 cgtgatcctc gtagtcgacc tcgatgtcga cgcaggtctc gcccggcgcg acgccgatga
     1141 tcacgaagcg acgcggcgcc tcggtcggct cgacgcgcaa gatgtcgcgg tcctgcgagg
     1201 tcagcaccag ctggtcgctc tcgtagtagt cgaactggtt ggagctcagc ggcctcacgt
     1261 cgaccgccgc ggcgacaccc gccgcgactt cgatctcctc gccgttgacc cgcaccggca
     1321 ccggcggcgc cgagtacagc tcgagcacga cgccgtcgta acgcgggccg caagcggtga
     1381 gcgcgagcag ggggagcatg aacaggctgt acaggcgctt cacaggacac ctcccgacga
     1441 gccgctcgag tcgccgcccg tgtcaccgct ggatccgctg gattcaccgc tcgagtcgct
     1501 gctggcgtcg ccgttcgagt cgccgctgct gctggactcg ctgccggact cgctgccggt
     1561 ctcgtcgtcc tcgccctcga cggtgatcgt cagctgcacg ctcatcgcgt catgggcgag
     1621 ggtgagggtg gtggtgccgc tctggagggc ctcgatgcgc gcgcggccgg agtcgccgcc
     1681 ggcgacgatg ctgacgatcg tcgggtcggc gctggtccag gtgtggccga gcacgccgcc
     1741 gagcagcacg ctgtcggagt cgacggcgat cgcctgcagc tcgcgctcgc cgccgcggtc
     1801 gagcgtcagc tgctgcaagg gcgcggcgcc gtgctggtcg gcgggcacga cctgcagctc
     1861 ggcgatctcg cggatgtgca ggtgtacgag gtcgacgatc tcgttgttgc cggtgacggc
     1921 gagcaccgcg gcgtagccgg tcgccagggc ggtgaagccg tcgcctttgc gggtgagcag
     1981 cgagggcgcg gccgactgca gctgcggcgg cgggtcgccg atgtgctccg cgtcgtcgcg
     2041 ccagtcgtac tcgaggttga tgcggccgcc gagggcgaag gcctgcggga attggtcgcc
     2101 ggtgccggtg ccgtcgctgc agaacgggtc gccgctgctc gggcactcgt acaggaacac
     2161 gccgacgccg agctcgccgt acttggggcc ggggttgggg tcgtaggtgt cgctgcaggc
     2221 cgtcagcccg agcgccagga cgtaggaggt ctttcgcatc gctgtgtgct cgcagtccaa
     2281 gtcaagggta agtggatcga cgtggttcga agtagatcgc ggcccgtccg gtggcggacg
     2341 aggcgtgacg cacacccatg gtagcaaagc cccaggcccg gggccctggg tccacagcgc
     2401 gaaaattacg taggcagtcc cgctagatgg ccggaggcgg tgcacctggc acgggaggtc
     2461 gtgtcacatg ggtgtgacaa agtcgtcacc ggtcgtacgc gtttgatccg tcgatgtgac
     2521 acgacagcgc gcatgcgccc ggattagggt ggcctggcac cgatgtccag gagcacgtga
     2581 catggaagcg ctgttcgacg caagtctcgg gaccggcacg ctgttgctgc tcatcctctc
     2641 gctgctgatc gcgttcggct tcgagttcgt caacggcttc cacgacaccg cgaacgcggt
     2701 ggcgacggtg atctacaccc ggtcgctgcg gccgtggacg gcggtgatcc tgtcgggggt
     2761 ctgcaacttc ctcggggtgt tcatcggcgg gatcgcggtg gcgatgagca tcatcagcct
     2821 gctgccggtc gagctgttgg tcgcgagcgg caccggggcc gggctggcga tgctgctcgc
     2881 gctgctggtc gcggcgatcg tgtggaacct cgggacctgg tacctcgggc tgccggcctc
     2941 gagctcgcac acgctgatcg gggcgatcgt cggggtcggg ctcgccaact cgctgctgcc
     3001 gggccatcag ttcggcgcgg gcgtcaactg ggccaaggcc ggcgacgtcg ggctggcgct
     3061 gctggtctcg ccgctggtcg gcttcaccgt cgcggcgctg ctgttgctcg cggcgaagaa
     3121 gctgatccgc gacccgcagc tgtaccgccc gccggtcggc acccagccgc cgccgcgctg
     3181 gatccgggcg gtgctgatcg gcacgtgcac gggggtcagc ttcgcgcacg ggtccaacga
     3241 cgggcaaaag ggcgtcggcc tggtgatgct ggtgctgatc ggcatcgtgc ccgcgggctt
     3301 cgccctcaac gtcggcatca gcgcgcccga cctcgcccgc gcgacccggg ccctcgacac
     3361 cgtcgag
//
LOCUS       DASWNJ010001409         1549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_518383, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001409 DASWNJ010000000
VERSION     DASWNJ010001409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518383"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VGB85_23460"
     CDS             <1..477
                     /locus_tag="VGB85_23460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137069.1"
                     /translation="EATAAASFAAVHAVFSGERSQVRVDTGPSPLTIAVGLACLAAIP
                     WVGRRGAQPVTSRAPAATGAGVRAPVIALVLAVLVAATVLANVLALRASGRLHLRCER
                     RCEAGGGTCMPGGEVTLTLPPGEHEVRVYDADAPDAPVVHRVALVAGELVEFTCAR"
     gene            545..949
                     /locus_tag="VGB85_23465"
     CDS             545..949
                     /locus_tag="VGB85_23465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137070.1"
                     /translation="MKEGDDRPEPQIELAGQHRVEIRRAPGSDTLELIGRDGRLVLAI
                     EISERGPVLRFEGPALTLRASGELALEARHLTLRADESLALVSGGDLHLVAAGDLHST
                     ARIQTITAELGDVKIEANDDVRIDGERVLVNC"
     gene            956..>1549
                     /locus_tag="VGB85_23470"
     CDS             956..>1549
                     /locus_tag="VGB85_23470"
                     /inference="COORDINATES: protein motif:HMM:NF021456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HEY0137071.1"
                     /translation="MKLENHTPYHAQLFRGSVDERRQFGSVVARLTYDIVGDRLRLSS
                     EQAWAVSPGPWESEHGVLPGDDLFYRGGVDLLVFGAARPTGGRPASRVDVDIRLGADF
                     RARLAVFGDRRWQRVGGRLVPGAPQPFRATPLTLAHAFGGQIEWDGLAVPFPDNPAGR
                     GFHVDEASAEGGLLPNIEDPAALIQRWDDRPEPVGTGA"
BASE COUNT          187 a          576 c          580 g          206 t
ORIGIN      
        1 gaggcgaccg cggcggccag cttcgcggcg gtgcatgcag tcttctcggg cgagcgctcg
       61 caggtccgcg tcgatacggg accctcgccg ctgacgatcg cggtcgggct cgcatgcctg
      121 gcggcgatcc cgtgggtcgg ccgtcgcggc gcgcagccgg tcacctcgcg cgcgccggca
      181 gcgaccggcg ccggcgtgcg cgccccggtg atcgcgctcg tgctcgccgt cctcgtcgcg
      241 gccaccgtgc tcgccaacgt cctcgccttg cgcgccagcg gccgcctgca cctgcgctgc
      301 gagcgacgct gcgaggccgg cggcggcacc tgcatgccgg gcggcgaggt gaccctgaca
      361 ctgccgccgg gcgagcacga ggtgcgcgtt tacgacgcgg atgccccgga cgcgccggtc
      421 gtgcaccgcg tcgcgctggt cgccggtgag ctcgtcgagt tcacctgcgc gcgctgacgc
      481 gggcgatctc gccttggtac gcgacgccga tccggcccgg cgataagtgt gaaaagatgt
      541 cgccatgaaa gagggggacg accgcccgga gccgcagatc gagctcgccg ggcagcatcg
      601 cgtggagatc cgccgcgcgc ccggcagcga cacgctcgag ctgatcggcc gcgacggccg
      661 gctggtcctg gcgatcgaga tcagcgagcg tggaccggtg ctgcgcttcg agggcccggc
      721 gctgacgctg cgggcctccg gcgagctcgc gctcgaggcc aggcacctga ccctgcgcgc
      781 cgacgagtcg ctggcgctgg tctccggggg cgacctgcac ctggtcgccg cgggcgacct
      841 gcacagcacg gcccgcatcc agacgatcac ggccgagctc ggcgacgtca agatcgaggc
      901 caacgacgac gtgcggatcg acggcgagcg ggtcctcgtc aactgctgag gcgtgatgaa
      961 gctcgagaac cacacgccgt accacgccca gctgtttcgc ggctccgtcg acgagcggcg
     1021 gcagttcggc tcggtcgtcg cgcgcctgac ctacgacatc gtcggcgacc gcctgcgcct
     1081 gtccagcgag caggcctggg cggtgtcgcc gggcccgtgg gagagcgagc acggggtgct
     1141 gcccggcgac gacctgttct accgcggcgg cgtcgacctg ctggtcttcg gcgccgcgcg
     1201 accgacgggc ggtcgacctg cgtcgcgggt cgacgtcgac atccgcctcg gcgcggactt
     1261 tcgcgcccgc ctcgcggtgt tcggcgatcg ccgctggcaa cgggtcggcg gccgcctggt
     1321 cccgggcgcg ccgcagccgt ttcgcgcgac gccgctgacc ctcgcgcacg cgttcggcgg
     1381 tcagatcgag tgggacggcc tcgccgtgcc gttcccggac aacccggcgg gtcgcggctt
     1441 ccacgtcgac gaggccagcg ccgagggcgg cctgctcccg aacatcgagg accccgcggc
     1501 gctgatccag cgctgggacg accgccccga gccggtcggc accggcgcg
//
LOCUS       DASWNJ010001410         2538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_164706, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001410 DASWNJ010000000
VERSION     DASWNJ010001410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164706"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VGB85_23475"
     CDS             <1..224
                     /locus_tag="VGB85_23475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137072.1"
                     /translation="LPEHTASPEHATSLPEHTASPEHATSLPEHTASPEHATSLPERP
                     EPEHEHPEPEHPEPDPQAARSERGEQAGM"
     gene            complement(236..1270)
                     /gene="fbaA"
                     /locus_tag="VGB85_23480"
     CDS             complement(236..1270)
                     /gene="fbaA"
                     /locus_tag="VGB85_23480"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEY0137073.1"
                     /translation="MPVADYATYCRMLDHARTEKFAYPAINITSSSTINAALAAFAEA
                     KSDGILQVSTGGGEFAAGQFIKDMAVGAMALAEYIHRVAAHYPINVAIHTDHCQADKV
                     DKFIRPLLAETRRRRASGQPNLFQSHMFDGSALPLAENMTIAQQLLRECAELEVILEV
                     EAGVVGGEEDGVNHEGAPAEKLYTTPEDMVSVARTLGAVEGGRYMFAATFGNVHGVYK
                     PGNVKLKPTILRDGQQAVAKEFGDKARFDLVFHGGSGSELKDIHETLEYGVVKMNVDT
                     DAQYAYTRPIAEHMFKNYDGVLRIDGEVGNKKHYDPRAYLKAAEKGMQARVRQACEDL
                     RSVGTTLHRR"
     gene            complement(1305..>2538)
                     /locus_tag="VGB85_23485"
     CDS             complement(1305..>2538)
                     /locus_tag="VGB85_23485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY0137074.1"
                     /translation="PRSGARELAWQAYGTHFLLAAVFKLFGPEPPYRAAAVLWATLGA
                     AAVPLTYLLACRILPTARLAAIAGITALCWYPNLSTTGYFLSETPFLCLQLLSAYCLV
                     RVIQDGKGALLAGVASAMCFMLRPQSAIFFVGVFGLWLINFRTLPWVRVKQLAGVGVP
                     LILALAFSMWRYHSHTGRWDGIAENANMNLTAGRCHNIVTQAFKTQADLDKSVKKNKT
                     SDGRRVSLPSYRTLDATFPPGHPLALNPAMESETIRFVGYIGDPWIHRDLRRECYRRT
                     GILGQIQYSITNLLLQWFISRQWPDNAKNRQFFLPPSEVFRYGFQVFVLLPSLVGVGL
                     GMARLRRDPALALVALCLLTSMVLAAVFFGDVRLRSPYDPYAIILALLAVHWFIERRR
                     QRKAAAAVVAPPPAAATP"
BASE COUNT          419 a          840 c          893 g          386 t
ORIGIN      
        1 cgctgcccga gcacaccgcg tctcccgaac acgcaacatc gctgcccgag cacaccgcgt
       61 ctcccgaaca cgcaacatcg ctgcccgagc acaccgcgtc tcccgaacac gcaacatcgc
      121 tgcccgagcg ccccgagccc gaacacgagc accccgagcc cgaacacccc gagcccgatc
      181 cacaagccgc gcgcagcgaa cgaggcgaac aggcaggcat gtgacgggcg gccgtttagc
      241 ggcgatgcag cgtggtgccg accgagcgca ggtcctcgca ggcctggcgg acgcgcgcct
      301 gcatgccctt ttcggctgcc ttgaggtaag cgcgcgggtc gtagtgcttc ttgttaccga
      361 cctcgccgtc gatgcgcagc acgccgtcgt aattcttgaa catgtgctcg gcgatcggtc
      421 gggtgtaggc gtactgggcg tcggtgtcga cgttcatctt gaccacgccg tactcgaggg
      481 tctcgtggat gtccttgagc tcggagccgg agccgccgtg gaacacgagg tcgaagcggg
      541 ccttgtcgcc gaattccttg gcgaccgcct gttgcccgtc gcgcaggatc gtcggcttga
      601 gcttgacgtt gccgggcttg tagacgccgt gcacgttgcc gaaagtcgcg gcgaacatgt
      661 agcggccgcc ctcgaccgcg ccgagcgtgc gggccacgct gaccatgtcc tcgggggtcg
      721 tgtacagctt ctcggccggg gcgccctcgt ggttgacgcc gtcctcttca ccgccgacga
      781 cgccggcctc gacctcgagg atcacctcga gctcggcgca ctcgcgcagc agctgctggg
      841 cgatcgtcat gttctcggcg agcggcaggg ccgagccgtc gaacatgtgc gactggaaca
      901 ggttgggctg gcctgaagcg cgtcggcggc gggtctcggc gagcagcggg cggatgaact
      961 tgtcgacctt gtcggcctgg cagtggtcgg tgtggatcgc cacgttgatc gggtagtgcg
     1021 cggcgacgcg gtggatgtac tccgcgaggg ccatcgcgcc gaccgccata tctttaataa
     1081 actggccggc agcgaactcg ccgccgccgg tgctcacctg caagatgccg tcgctcttgg
     1141 cctcggcgaa ggccgcgagc gcggcgttga tcgtgctcga cgaggtgatg ttgatcgccg
     1201 gatacgcgaa cttctcggtc cgggcgtggt cgagcatcct gcagtacgtg gcgtagtcgg
     1261 cgacgggcat ggcgggccat gcgtaccaca ggcgccgcgg gtcgtcacgg tgtcgcggcg
     1321 gcgggcggcg gcgcgacgac ggccgcggcc gctttacgct ggcgccggcg ctcgatgaac
     1381 cagtgcaccg caagcagcgc caggatgatc gcgtaggggt cgtaggggct gcgcagccgc
     1441 acatcgccga agaacaccgc cgcgagcacc atggaagtca gcaggcacag ggcgaccagc
     1501 gccagcgccg ggtcccgccg caggcgcgcc atgccgagcc cgaccccgac cagcgagggc
     1561 agcagcacga acacctggaa gccgtagcga aacacctccg agggcggcaa aaagaattgc
     1621 cggttcttgg cgttgtcggg ccactgccgc gagatgaacc actgcagcag caagttggtg
     1681 atcgagtact ggatctgccc gaggatcccc gtacgtcggt agcactcgcg ccgcaagtcg
     1741 cggtggatcc acgggtcgcc gatgtagccg acgaagcgga tcgtctccga ctccatcgcc
     1801 gggttgaggg cgagcgggtg gccgggtggg aaggtggcgt cgagcgtgcg gtagctcggc
     1861 aggctgaccc ggcggccgtc gctggtcttg ttcttcttga cgctcttgtc gaggtcggcc
     1921 tgggtcttga acgcctgcgt gacgatgttg tggcagcgcc ccgccgtcag gttcatgttg
     1981 gcgttctcgg cgatgccgtc ccagcgcccg gtgtggctgt ggtagcgcca catgctaaat
     2041 gccagcgcca ggatcagcgg cacgccgacg cccgccagtt gtttgacccg cacccacggc
     2101 agcgtgcgaa agttgatcag ccacaggccg aacacgccga cgaagaagat cgccgactgc
     2161 gggcgcagca tgaagcacat cgccgaggcg acgccggcca gcagcgcgcc cttgccgtcc
     2221 tggatcacgc ggacgaggca gtaggcggac agcagctgca ggcacaaaaa tggcgtctcc
     2281 gacaggaagt atccggtcgt cgacaggttc gggtaccagc acagcgcggt gatgccggcg
     2341 atcgcggcga gccgcgcggt cggcaagatc cggcaggcga gcagataggt cagcggcacg
     2401 gccgcggccc cgagggtggc ccagagcaca gccgcggcgc ggtacggcgg ctccggaccg
     2461 aacagcttga acaccgccgc gagcaggaag tgcgtgccgt acgcctgcca cgccagctcg
     2521 cgggcgccgc tgcgcgga
//
LOCUS       DASWNJ010001411         2251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_25992, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001411 DASWNJ010000000
VERSION     DASWNJ010001411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25992"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1666
                     /locus_tag="VGB85_23490"
     CDS             <1..1666
                     /locus_tag="VGB85_23490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HEY0137075.1"
                     /translation="GMVVVAAIEHYPSDTRDPLLVRVERVLGPPDTLGTEQLKILIER
                     GINPEFPDAVLAEAQGVPTAVQPDELVGRDDLRNLDFMTIDPVDARDFDDAVCVELLG
                     DNLHTDDMRIHIAVADVSHYVREGTAIDDEAALRCFSAYLPDRAIPMLPHALSSHMCS
                     LVPNEDRLSMVISMRLDPAGETTEVKLRAAVIHSRKRLSYEQVAAELDQPSGAGKLEE
                     EVIARIKLLRRAADRLRAMRLRRGAVELNLPETKILLDEDDPSRIRDIVPSRASESVA
                     RAYNLIEELMLAANEANGQIAVEHKLPMVFRVHDLPDTDKLTLLAEAAAVLGMKVDPE
                     KLTTPRGVQKFLALAESQPRGTALHMFMLRAMAQAAYSCDNVGHFALASSAYVHFTSP
                     IRRYPDLVAHRVLKAHLGRTGGQAGPEPVPAMPALEASQAQAARSSSRERATADAERD
                     SKALFAAAYMRDRLGDRFEATITGFGSAGMYILLDRPFVDGMVRLGALERERGELYEL
                     EDNHVRLVGKRSGHVLMVGDRIVVEAIDASLQRRKVDFAYISKLTA"
     gene            complement(1810..>2251)
                     /locus_tag="VGB85_23495"
     CDS             complement(1810..>2251)
                     /locus_tag="VGB85_23495"
                     /inference="COORDINATES: protein motif:HMM:NF040596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MXAN_2562 family outer membrane beta-barrel
                     protein"
                     /protein_id="HEY0137076.1"
                     /translation="AEGSVRSAADSTRFAVMPLALQAVYRFELLADRFRVPIVPYGKF
                     GLNYTFWWSKNGSGDISTIKDENGKVLDKARGGAWGYQANVGGMLRLDWLERGEARNL
                     DRITGINHTYLFAEWQFSRVNNFGRKNSISLGDSTWLVGLAIEF"
BASE COUNT          353 a          751 c          817 g          330 t
ORIGIN      
        1 gggcatggtc gtcgttgcgg cgatcgaaca ctatccgagc gacacgcgcg acccgctgct
       61 ggtccgcgtc gagcgtgtgc tcgggccgcc ggacaccctc ggcaccgagc agctcaagat
      121 cctgatcgag cgcgggatca accccgagtt ccccgatgcc gtgctggcgg aggcgcaggg
      181 cgtgccgacg gcggtgcagc cggacgagct cgtcgggcgc gacgacctgc gcaacctcga
      241 cttcatgacg atcgacccgg tcgacgcccg cgacttcgac gacgcggtgt gcgtcgagct
      301 gctcggcgac aacctgcata ccgacgacat gcggatccac atcgcggtcg cggatgtgtc
      361 gcattacgtc cgcgagggca cggcgatcga cgacgaggcg gcgctgcgct gcttctcggc
      421 gtacctgccg gaccgcgcga tcccgatgct gccgcacgcg ctcagctcgc acatgtgctc
      481 gctggtgccg aacgaggacc gcctgtcgat ggtgatcagc atgcgcctcg accccgccgg
      541 cgagacgacc gaggtcaagc tgcgcgccgc ggtgatccac agccgcaagc gtctcagcta
      601 cgagcaggtc gcggcggagc tcgaccagcc ttccggggcc ggcaagctcg aagaggaggt
      661 gatcgcgcgg atcaagctgc tgcgccgcgc ggccgaccgc ctgcgggcga tgcgtctgcg
      721 ccgcggcgcg gtcgagctca acctgcccga gacgaagatc ctcctcgacg aggacgaccc
      781 gagccggatc cgcgacatcg tgccatcgcg cgcgagcgag tcggtcgccc gcgcctacaa
      841 cctgatcgag gagctgatgc tcgcggccaa cgaggccaac ggtcagatcg cggtcgagca
      901 caagctgccg atggtgttcc gcgtgcacga cctgccggac accgacaagc tgacgctact
      961 cgctgaggca gccgctgtgc tcggcatgaa ggtggacccg gagaagctga cgacgccgcg
     1021 cggggtccag aagttcctcg cgctggcgga gagccagccg cgcggcacgg ccctgcacat
     1081 gttcatgctg cgggcgatgg cccaggcggc gtacagctgc gacaacgtcg gccacttcgc
     1141 gctggcgagc tcggcctacg tgcacttcac gtcgccgatc cgtcgctacc cggacctggt
     1201 ggcgcaccgc gtgctcaagg cccacctcgg acgcacaggc ggccaggcgg ggcccgagcc
     1261 ggtgccggcg atgccggcgc tggaggccag ccaggcccag gcggcgcgca gcagctcgcg
     1321 tgagcgggcg accgcggacg ccgagcgcga cagcaaggcg ctgttcgccg cggcgtatat
     1381 gcgcgaccgc ctcggcgacc gcttcgaggc gacgatcacg ggctttggaa gtgcgggcat
     1441 gtacatcctg ctcgaccggc cgttcgtcga cgggatggtc cgcctcgggg cgctcgagcg
     1501 cgagcgcggc gagctgtacg agctcgagga caatcacgtg cggctcgtcg gcaagcgctc
     1561 cgggcacgtg ctgatggtcg gggaccgcat cgtcgtcgag gcgatcgatg cgtcgttgca
     1621 gcggcgtaag gtcgactttg cttatattag taagttgact gcgtagtcgg ctgggcgcag
     1681 ggacccgccg tccgtcggcc ccccccggcc caggaagcct cgccccgcgt tgttcgaacg
     1741 tgccatgagg caccacgaac gcgctgcgag ccacgagcgt gccgtgtgaa cgcgggagcg
     1801 gggccgaggt cagaattcga tcgcgaggcc gacgagccag gtggagtcgc cgaggctgat
     1861 gctgttcttg cggccgaagt tgttgacgcg cgagaactgc cactcggcga acaggtaggt
     1921 gtggttgatg ccggtgatgc ggtcgaggtt gcgggcctcg ccgcgctcga gccagtcgag
     1981 gcggagcatg ccgccgacgt tggcctggta gccccaggcg ccgccgcggg ccttgtcgag
     2041 gaccttgccg ttttcgtcct tgatcgtcga gatgtcgccg ctgccgttct tcgaccacca
     2101 gaacgtgtag ttgaggccga acttgccgta ggggacgatc gggacgcgga agcggtcggc
     2161 gagcagctcg aagcggtaga cggcctggag ggcgagcggc atcacggcga agcgcgtgct
     2221 gtcggcggcg ctgcgcacgg agccctcggc g
//
LOCUS       DASWNJ010001412         2552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_308863, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001412 DASWNJ010000000
VERSION     DASWNJ010001412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308863"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VGB85_23500"
     CDS             complement(<1..793)
                     /locus_tag="VGB85_23500"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0137077.1"
                     /translation="MTRILFLTASLVLVPACFVQEIEDIIANHTSATMGSTGGTSTGG
                     TSTSGTSTGRPGTGTGTGGEADTLGDTHDPSSGGLGGTGTGTTQADTAAESSTGTAPL
                     CGDGLIEGDEECDDANTVDADGCLNNCTREWLVFVTSDPGTQGHFGGVIGADYECRHR
                     ATKLFLPNGERYKAWISTSLVQPVDRLYHARGPYKLVNGLRVAANWDALVSGSLENPI
                     NVTELGHTSEGAVFTGTLPDGTRAPDSTHCDGWTDNDGDNFGWYGA"
     gene            complement(809..1927)
                     /locus_tag="VGB85_23505"
     CDS             complement(809..1927)
                     /locus_tag="VGB85_23505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137078.1"
                     /translation="MSVAWLLSALGCPANPVSPDPPEGASDDDDGDDDDDEPDVDETP
                     TSGPTTTATTGTDTGTGEPGPASSETTDAATDSDTSATETTATSVPSGTCGDGVVDPG
                     ETCDLTYAENKDTGACTKSCQLPYCGDGLVWEGEESCDNGMANNDFLYGGCRENCTPG
                     PSCNDGLLQPEEECDASAPAAEGAVGCDPGNCRMMARVAFVTAAEFSGDLGGLAGADA
                     TCVAAAAAAKLDNAGSFLAYMGDGAAAPANRFVDGLADKGYPYARRDGQKLANDLDDL
                     FDIGLRVPLVITELGTTLPPEQLAWTGVDIHGEPGGAHCEAWSTDYFKYSGRVGQISP
                     ATDSDADIFAWKINGDWVDYASKSCFTNDARHLYCFED"
     gene            complement(2235..>2552)
                     /locus_tag="VGB85_23510"
     CDS             complement(2235..>2552)
                     /locus_tag="VGB85_23510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137079.1"
                     /translation="SFRSTLQLPSVPHVEGAGFEHKIPGLEVRHTVDVRGSCVTVVDT
                     VKLCCHAIADVDAVLAEIRRAPQRLRVRLRPFAPASVAHTTRATDTPARTGAARPFRP
                     RFR"
BASE COUNT          368 a          824 c          913 g          447 t
ORIGIN      
        1 tggcgccgta ccacccgaag ttgtcgccgt cgttgtcagt ccagccgtcg cagtgggttg
       61 agtccggcgc ccgcgtcccg tcgggcaggg tgcccgtgaa caccgctccc tcggaggtat
      121 ggccgagctc agtgacgttg atggggtttt cgagggagcc gctgacgagg gcgtcccagt
      181 tggcggcgac gcgcaggccg ttgacgagct tgtagggccc gcgggcgtgg tagaggcggt
      241 cgacgggctg gaccaggctg gtgctgatcc aggccttgta gcgctcgccg ttggggagaa
      301 agagcttggt ggcgcggtgg cggcactcat agtcggcccc gatgacgccg ccgaagtggc
      361 cctgggtgcc cgggtcgctg gtgacgaaga cgagccactc gcgggtgcag ttgttgaggc
      421 aaccatccgc gtcgacggtg ttggcgtcgt cgcactcctc gtcgccctcg atcaggccgt
      481 cgccgcacag cggcgcggtg ccggtcgacg actcggcggc ggtgtcggcc tgtgtggtgc
      541 cggtgccggt gccgccgagg ccgcccgagg aggggtcgtg cgtgtcaccc agcgtgtcgg
      601 cctctccgcc ggtgcccgtg ccggtgccgg gtcttccagt cgaggtgccc gacgtcgagg
      661 tgcccccggt cgaggtgccc ccggtcgagc ccatggtggc cgacgtgtga ttggcgatga
      721 tgtcttcgat ctcctggacg aagcaggcgg ggacgagcac gagcgaggcg gtcagaaaga
      781 ggatgcgcgt catggtggtc tccggtcgtc agtcttcgaa gcagtacaga tgcctggcgt
      841 cgttcgtgaa gcacgatttt gaggcgtagt cgacccagtc gccgttgatc ttccaggcga
      901 agatgtcggc gtcgctgtcg gtcgccgggg agatctgccc gacccgcccg gagtatttaa
      961 aatagtcggt gctccacgcc tcgcagtgcg ccccgccggg ctcgccatgg atgtcgacgc
     1021 cggtccaggc gagttgctcc ggcgggaggg tcgtgccgag ctccgtgatc accagcggga
     1081 cccgcagccc gatgtcgaac aggtcgtcga ggtcgttggc cagcttttgc ccgtcacggc
     1141 gggcgtaggg gtaacccttg tccgcgaggc cgtcgacgaa gcggttcgcg ggcgcggcgg
     1201 cgccgtcgcc catgtacgcg aggaagctgc cggcgttgtc gagtttggcg gcggccgcgg
     1261 cggccacaca ggtggcgtcc gccccggcga gcccgccgag gtccccggag aactcggcgg
     1321 cggtgacgaa ggcgaccctg gccatcatcc ggcaatttcc cgggtcacag ccgaccgctc
     1381 cttcggcggc tggcgcgctg gcatcgcact cctcctcggg ctggagcaaa ccgtcattac
     1441 agctcggccc tggcgtacag ttttcacggc atccgccgta taaaaaatca ttgttggcca
     1501 tgccgttgtc gcacgattcc tcgccctccc acaccaggcc gtcgccacaa tacgggagct
     1561 ggcaactctt ggtgcaggcg ccggtgtcct tgttttcggc gtaggtaagg tcgcaggttt
     1621 ctccggggtc cacgacccca tcgccgcagg tcccggatgg gacgctggtc gccgtggtct
     1681 cggtcgccga ggtgtccgag tcggtcgcag cgtcggtcgt ctcgctgctc gccggacccg
     1741 gctcgccggt ccccgtgtcg gtgcccgtgg tggcggtcgt cgtcggcccc gaggtcggcg
     1801 tctcgtcgac atccggctcg tcatcatcgt catcgccatc atcgtcgtcc gaagcgcctt
     1861 cgggcgggtc cggggacacc gggttcgccg ggcacccgag cgccgacaac agccacgcga
     1921 ccgacatcga cctgtgaagc gcacatcgca taccggccac gtctcgcata actattcgag
     1981 gcagtcaaag cctttttcca atactattac tctggcgtca gattgcgtaa gactccttac
     2041 agttaattca cgatgtgaag cgccgaaggc tggaaacgga gttctcgcgg tcgcgccacc
     2101 cggccgtcgc ggagtccgga caggtagtcg gccgagagcg cctcccgggt tcacgagcca
     2161 tccccgtcga gacgagatcc agggacgatc atgacgcgcc gggtccgggc ggctctcctc
     2221 gatgctggcc tggatcagcg aaaccggggg cggaaggggc gggccgctcc cgttcgtgcg
     2281 ggcgtgtcgg tcgcacgggt cgtatgggcg acgctggcgg gcgcgaacgg acgcagccgg
     2341 acgcgaagcc gctgcggggc gcgccggatc tcggcgagca cggcgtcgac atccgcgatc
     2401 gcatggcagc acagcttcac cgtgtccacg acggtcacgc aggagccacg gacatcgacg
     2461 gtgtgccgga cctcgagccc ggggatcttg tgttcgaacc cggcgccctc gacatgaggg
     2521 acggatggca gctgcagcgt ggacctgaat ga
//
LOCUS       DASWNJ010001413         4017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_236491, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001413 DASWNJ010000000
VERSION     DASWNJ010001413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236491"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..373)
                     /locus_tag="VGB85_23515"
     CDS             complement(110..373)
                     /locus_tag="VGB85_23515"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY0137080.1"
                     /translation="MAIIWTLLIGLVAGALAKLIMPGRDPGGILVTMLLGVAGAFVAG
                     FLGKAFGWYNDVGEGPGLIASIVGALIILAGYRAIVGMRTRNG"
     gene            complement(607..774)
                     /locus_tag="VGB85_23520"
     CDS             complement(607..774)
                     /locus_tag="VGB85_23520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137081.1"
                     /translation="MGEPADAITLGAKEHSMFALSTLSILTVVMCFNLALLLSLGLYL
                     QEQRHAAPPQR"
     gene            894..1823
                     /locus_tag="VGB85_23525"
     CDS             894..1823
                     /locus_tag="VGB85_23525"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY0137082.1"
                     /translation="MASPPLYIVGAGAVGLHLAARLSAITPVTVVARGPRAARLAREG
                     FDLAGAEQGHFALPVVDIADPLPASAELLIAVKSTQLRETLHQLRPGPQHTLGLCQTG
                     LGVAAMARALAPTAARVRLGCWIAAGLATPTTVRVAGIYQVELAADDPEALRTRDRWQ
                     RLLASAGYPVRVVSSVAACEWRKALLNLAVNGLCATLGERTGAVVDSPPLHALAREIL
                     QEARSVAAAEGVRLTDEDIERVFTSATNLRNDRSTMLQDVTRGEATEMQFLNLAVARL
                     AVKHGLFAPVNAVVARLVEHCERRRPDPGPSAA"
     gene            complement(1835..2998)
                     /locus_tag="VGB85_23530"
     CDS             complement(1835..2998)
                     /locus_tag="VGB85_23530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137083.1"
                     /translation="MSSTRPAGYFRRTAVSMLMVACSSSSEETSGFSGSTPTASSATS
                     STTAGPDPTEAPATDAGTAGISATGTGDPTSTASSTATGEATDGGPKLDLGVSVDFGG
                     NPGQGCSKVDFLFVVDNSISMGDEQQNLATSFPSFISTIQSQVAADDYHIMMIDTDDL
                     DKWGEKWNKCHEKCMTDDPGDGCLTVYFDDIICGMEPPPPEACDQTLGAGRNKGAGGP
                     PVVCPIDGGGRFMTQDQTDLAATFDCVAKMGATGNSNERPAAALLAALGEQTQAGGCH
                     PGFLRDDAVLVLTIISDEEEIGSPGDPKSWYAELLARKQGNTTSIVSLVLSGDTNIMG
                     QECTPTPKWTELTDLVGPERGIIESICQPDYGPFFTTAVSVIDYACDTFVPPG"
     gene            complement(3080..3787)
                     /locus_tag="VGB85_23535"
     CDS             complement(3080..3787)
                     /locus_tag="VGB85_23535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137084.1"
                     /translation="MWIAVIVLLVFALVAVLATIFGLVWFFKRAERINRALKKIPVTR
                     VAVAQPGTMARVIGKLKPAQGPLLRAPLSGKPCGYFQAVAEEWRASGNSEGWVQLAYE
                     EQWCDFCLDDGTGQILVRMLRPQISADLEEGSFSDPILPPNPQQESFLRRHKAWQPAE
                     GKPQPRLRYLESLFELDEEITAYGIVRLEREGPGFGSVRVVIEAPPGQPLLVSDHKQI
                     TRSPSPASTMLSMSSIR"
BASE COUNT          603 a         1478 c         1317 g          619 t
ORIGIN      
        1 gtctcacgtg cgaactcggt cacgcctcgc gaaaacgagg accctcaccc cggtgggggc
       61 gatggtcctc gtgttccgcg ctttccggag cgcagcggag cgcagcgggt cagccgttgc
      121 gggttcgcat gccgacgatc gcgcggtaac cggccaggat gatcagcgcg ccgacgatcg
      181 aggcgatcag gcccgggccc tcgccgacat cgttgtacca gccgaacgcc ttgcccagga
      241 aacccgccac gaaggcacca gccacgccga gcagcatcgt cacgaggatg ccacccgggt
      301 cacgacccgg catgatgagc ttggcaaggg cgccagcgac gagtccgatt aacagtgtcc
      361 agatgattgc cattgtcttg cgctccggtg gaggtggatg cctcgcaacc tagcgcgcac
      421 cggaaggcgt gcaaccggct ttcttcgccg ccgcccctgc atgcgccgcc agcagcccgt
      481 gcgcagccct ccgcccgtcc cggtcctccg tcgcgcgacg ccggggcgcc atcaccgcca
      541 aacgcagaac aacaatcctg ctccgcactt tccccccaca tgtcccgaag gacatgtacc
      601 tcgccgctag cgctgcggcg gcgcggcgtg gcgctgctcc tggagataca agcccagcga
      661 aagcaacaag gcgaggttga aacacatgac gacggtgagg atcgacaggg tcgagagggc
      721 gaacatgctg tgctccttgg cccccaatgt gatcgcgtcg gccggctcac ccaagcgaag
      781 gattttccgc gccccacggc gcatcgcgca gcgcttacga ggtctgcgtc acacggcttt
      841 ctcacgcccc gcgcgtcctg tggtcatggc gggcgtgtac catccccgcc gcgatggcct
      901 cgcccccgct ctacatcgtc ggcgccggcg cggtcggcct ccacctcgcc gcgcgcctct
      961 cggcgatcac gccggtcacc gtcgtcgcgc gcgggccccg ggccgcgcgt ttggcccgcg
     1021 agggcttcga cctcgcgggc gccgagcagg gtcatttcgc gctgccggtc gtcgacatcg
     1081 ccgacccgct gccggccagc gccgagttgc tgatcgccgt caagtcgacc cagctgcgcg
     1141 agaccctgca ccagctgcgc ccgggccccc agcacacgct cggcctgtgc cagaccggcc
     1201 tcggcgtcgc cgccatggcc cgcgcgctcg ccccgaccgc cgcccgcgtg cgcctcggct
     1261 gctggatcgc cgccggcctc gccaccccga ccaccgtccg cgtcgccggc atctatcagg
     1321 tcgagctcgc cgccgatgac cccgaggccc tgcgcacccg cgaccgctgg cagcggctgc
     1381 tcgcctccgc cggttacccc gtgcgcgtcg tctcgagcgt cgccgcctgc gagtggcgca
     1441 aggccctgct caacctcgcg gtcaacggtc tatgtgccac cctcggcgag cgcaccggcg
     1501 cggtcgtcga ctcccctccg ctgcacgccc tcgcccgcga gatcttgcag gaggcgcgca
     1561 gcgtcgccgc ggccgagggc gtgcgcctga ccgacgagga catcgagcgc gtcttcacct
     1621 cggccaccaa cctccgcaac gaccgcagca cgatgctgca agatgtcacc cgcggcgagg
     1681 cgaccgagat gcaattctta aatcttgccg tcgcccgcct ggccgtcaaa cacggcctgt
     1741 tcgccccggt caacgcggtg gtcgcccgcc tcgtcgagca ctgcgagcgc cgcaggcccg
     1801 accccggccc ctcggccgcg tgaatgcgcg caggtcagcc cggcggcacg aaggtgtcgc
     1861 aggcataatc gatcacgctg acggccgtcg tgaaaaatgg cccgtagtcg ggctggcaga
     1921 tgctctcgat gatcccacgc tcggggccga cgaggtcggt gagctcggtc cacttcgggg
     1981 tcggcgtgca ctcctgaccc atgatgttgg tgtcgccgct gagcaccagc gagacgatgc
     2041 tggtcgtgtt gccctgcttg cgcgcgagca gctccgcgta ccagctcttc gggtcgccgg
     2101 gtgagccgat ctcctcctcg tcggagatga tcgtcaacac cagcacggcg tcgtcgcgca
     2161 aaaagcccgg gtgacagccg cccgcctgcg tctgctcgcc gagggccgcc agcagcgccg
     2221 cggccggacg ctcgttcgag ttgccggtcg cgcccatctt cgcgacgcaa tcgaaggtcg
     2281 ccgccaggtc ggtctgatcc tgggtcatga agcggccgcc gccgtcgatc gggcacacga
     2341 ccggcgggcc gcctgcgccc ttgttacgac cggcgccgag cgtctgatcg cacgcctccg
     2401 ggggcggcgg ctccatgccg cagatgatgt cgtcaaaata caccgtcagg cagccgtcgc
     2461 ccgggtcgtc ggtcatgcac ttctcgtggc acttgttcca cttctcgccc cacttatcga
     2521 ggtcgtccgt gtcgatcatc atgatgtgat agtcgtcggc cgcgacctgc gactggatcg
     2581 tgctgataaa gctcgggaag ctggtggcga ggttctgctg ctcgtcgccc atcgagatcg
     2641 agttgtcgac gacgaacagg aagtccacct tggagcagcc ctgcccgggg ttgccgccga
     2701 agtcgacgct gacgccgagg tcgagcttcg ggccgccgtc ggtcgcctcg cccgtcgcgg
     2761 tgctcgacgc ggtgctcgtc gggtcaccgg tgccggtcgc gctgatcccc gcggtgccgg
     2821 cgtcggtcgc cggcgcctcg gtcggatccg gcccggccgt cgtgctcgac gtcgccgagg
     2881 acgccgtcgg cgtgctgccc gagaagccgc tcgtctcctc actcgaggac gaacacgcga
     2941 ccatcagcat cgagacagcg gtgcggcgaa agtatcctgc aggtcgggtc gaggacatgc
     3001 catcaggaat acacagacca cccgggcccg gtcacccctt ttgacgctcg cggggtcggg
     3061 gccggccgac cccacagcct cagcgaatcg acgacatcga cagcatcgtc gacgccggcg
     3121 acggcgatcg tgtgatctgc ttgtgatcac tgacgagcag cggctggccg ggcggagcct
     3181 cgatcacgac gcgcacgctg ccaaacccag ggccctcgcg ctcgaggcga acgatgccat
     3241 aggctgtgat ctcctcatcg agctcgaaca gactctccag ataacgcagg cgcggctgcg
     3301 gcttgccctc ggccggctgc caggctttat ggcggcgcag gaagctctct tgctggggat
     3361 tgggcgggag gatggggtcg gagaacgacc cctcctcgag gtcggccgag atctgcggcc
     3421 gcagcattcg cacgaggatc tggccggtgc cgtcatcgag gcagaagtcg caccactgct
     3481 cctcgtaggc gagctgcacc cagccctcgg agttgccgct cgcgcgccac tcctcggcga
     3541 ccgcctggaa gtacccgcag ggcttgcccg acagcggcgc gcgcagcaag ggtccttgcg
     3601 ccggtttgag cttgccgatc acgcgggcca tcgtgccggg ctgcgcgacc gcgacgcggg
     3661 tgacggggat cttcttcagc gcccggttga tccgctcggc ccgtttaaaa aaccaaacca
     3721 ggccgaatat cgtggcgagc accgccacga gggcaaacac cagcagtacg atcaccgcga
     3781 tccacatgga gcggcggcag gctaccacag cactgccggc gccgctcccg ggccggtcga
     3841 acgcgcgcag acccgccacg aacgctgttg gccggccctg tgccggtcat gccctgcggg
     3901 cgggccgcct tttagctctt gagggctcgc atcgcggcgg cgaggcgcgg gagcgcctcg
     3961 gcgatctcgg cctcgctggc ggcgccgacg ctgaggcgga accagccctc gtcgccg
//
LOCUS       DASWNJ010001414         1872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_164757, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001414 DASWNJ010000000
VERSION     DASWNJ010001414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164757"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..1295)
                     /locus_tag="VGB85_23540"
     CDS             complement(36..1295)
                     /locus_tag="VGB85_23540"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0137085.1"
                     /translation="MRRSVLPGGLRVVTEQVPGMRSAAFGIWVAVGSRDERPSIHGAS
                     HFLEHLLFKGTKRRDALEISSVMDAVGGEMNAFTAKEYTCYYARVLDEDLPLAVDVVS
                     DLVLSSVVTSDDVEVERGVILEEIAMHDDEPGDAVHDALAGALWGDSPLGRPVIGTGE
                     SIESRSRPAIAGFYRRRYDLPHMVVAAAGSVDHDVLVGLVEQAFAERLTGDAAPAPLR
                     APGPAPRAAARVVVQARATEQAHVVLGTTALRRDDPRRFAMSVLSGALGGGMSSRLFQ
                     SVREERGLAYSVYSYTDSHADTGLFGVNAGCSPGKVGEVLDLVRAELAAVAEHGVTDE
                     EVRRSQGNLRGSMVLGLEDTGSRMSRLGKAALVHEELLTPDEVLDRIAAVTPDEVRDV
                     ARDVLTRPRSLAVIGPYGTADLQEAIA"
     gene            complement(1360..>1872)
                     /locus_tag="VGB85_23545"
     CDS             complement(1360..>1872)
                     /locus_tag="VGB85_23545"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEY0137086.1"
                     /translation="MGEAIDAPDEMAPTAPRVLSVKIPVDKIGEVIGPKGKMINQIQA
                     DTGADITVEDDGTIYIGAADGPSAEAALATVNQIANPTMPKVGERFLGTVVKTAAFGA
                     FVSLLPGKDGLVHISKLGNGKRIAKVEDVVNVGDKLQVEITEIDQRGKISLTPVQNDA
                     PAAAEPVAAE"
BASE COUNT          244 a          692 c          672 g          264 t
ORIGIN      
        1 catgcgcccg cgcgctccca gcaccgccac gcgcgtcatg cgatcgcctc ctgcaggtcg
       61 gccgtgccgt acggcccgat gaccgccagc gagcggggcc gggtcaggac gtcgcgggcg
      121 acgtcgcgga cctcgtcggg ggtgaccgcg gcgatccggt cgagcacctc gtcgggggtg
      181 agcagctcct cgtgcaccag cgccgccttg cccagccggc tcatccgcga gccggtgtcc
      241 tcgaggccga ggaccatcga gccgcgcagg ttgccctggc tgcgccggac ctcctcgtcg
      301 gtgaccccgt gctcggcgac ggcggcgagc tcggcgcgca cgaggtcgag cacctcgccg
      361 accttgcccg gcgagcagcc ggcgttgacg ccgaacaggc ccgtgtcggc gtgcgagtcg
      421 gtgtaggagt agaccgagta ggccaggccg cgctcctcgc gcaccgactg gaacagccgg
      481 ctcgacatgc cgccgccgag cgcgccggac agcaccgaca tcgcgaagcg gcgcgggtcg
      541 tcgcggcgca gcgcggtcgt gccgagcacc acgtgggcct gctcggtcgc ccgcgcctgc
      601 acgacgacgc gggcagcggc ccgcggcgcc gggccgggcg cccgcagcgg cgcgggcgcc
      661 gcgtcgccgg tcagccgctc ggcgaaggcc tgctccacga gcccgacgag cacgtcgtgg
      721 tcgaccgagc cggccgcggc gacgaccatg tggggcaggt cgtagcgccg acggtagaag
      781 ccggcgatcg ccggccggga ccgcgactcg atgctctcgc cggtcccgat gacgggccgg
      841 ccgagcggcg agtcgcccca cagcgcgccg gcgagggcgt cgtggacggc gtcgcccggc
      901 tcgtcgtcgt gcatggcgat ctcctcgagg atcacgcccc gctcgacctc gacgtcgtcg
      961 gaagtgacca ccgacgacag gacgaggtcg ctgaccacgt cgaccgccag cggcaggtcc
     1021 tcgtcgagga cccgcgcgta gtagcaggtg tactccttgg cggtgaaggc gttcatctcc
     1081 ccgccgaccg cgtccatgac ggacgagatc tccagcgcgt cacgccgctt ggtgcctttg
     1141 aacagcaggt gctcgaggaa gtgcgaggcg ccgtggatcg acggccgctc gtcgcggctg
     1201 cccaccgcca cccagatgcc gaacgccgcc gagcgcatgc ccggcacctg ctccgtgacg
     1261 acacgcagcc ctcccggcag gaccgaccgg cgcaccgtgg cgggtgcgcc ggcaggtccc
     1321 actgccggga gggctcgcgt gcgtgctgac aaggaggaac tactcggccg cgaccggctc
     1381 ggccgccgcc ggcgcgtcgt tctgcaccgg cgtcaggctg atcttgccgc gctggtcgat
     1441 ctcggtgatc tcgacctgca gcttgtcgcc gacgttgacg acgtcctcga ccttcgcgat
     1501 gcgcttgccg ttgccgagct tgctgatgtg caccaggccg tccttgccgg gcagcaggct
     1561 cacgaacgcg ccgaaggctg cggtcttcac gaccgtgccc aggaagcgct cccccacctt
     1621 cggcatggtc gggttggcga tctggttgac cgtcgccagg gccgcctcgg ccgaggggcc
     1681 gtcggccgcg ccgatgtaga tcgtgccgtc gtcctcgacg gtgatgtccg caccggtgtc
     1741 ggcctggatc tggttgatca tcttgccctt cgggccgatg acctcgccga tcttgtcgac
     1801 agggatcttc accgacagga cgcgcggagc cgtgggcgcc atctcgtccg gggcgtcgat
     1861 cgcctcgccc at
//
LOCUS       DASWNJ010001415         1549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_518424, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001415 DASWNJ010000000
VERSION     DASWNJ010001415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518424"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="VGB85_23550"
     CDS             <1..881
                     /locus_tag="VGB85_23550"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0137087.1"
                     /translation="PSLTVSQMFSSGDRPTAWGDVDGDGLTDMMFLEDQAFSYHQGGG
                     GQLTLIATTPGMYAEFLLDVVDATGDGRGDLFDIADQVKFTTWPGDGLGGFTKGKTLM
                     LSDPAEVGSAVDKVFDRRGRLAMVSFYAPVCDVCTSRRIVTLVNDGTGFTNPAPPQTF
                     SQTVTDPVFADLDDDDVPELVMSNWSGGKNVEIHEMVTPGVLNLRGKLPGQKPRIGDI
                     DGDGRDDVLTSEKDHINIYLAATGYTAEPTPLVFPGIDEPVVALVADLDGDGIDDFVV
                     TALFGYDAVLVRSGCG"
     gene            961..>1549
                     /locus_tag="VGB85_23555"
     CDS             961..>1549
                     /locus_tag="VGB85_23555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137088.1"
                     /translation="MSRSGLLAPLTLTLALGACTPTPNPVAPGTHAGPPRRCAGQPAP
                     AVPVQAPAASPAPPLSPCPSCAPPPAPELPAPTGTIVVTAGERPIRVHFSGADEPYRD
                     CQAHAWVVRTVPAGVDPVAFAVRAVITGPTKFEAAQGLHTPFAAASSDPGIAPLSASG
                     VRVKVKDGVATVDFPTSARPYLDQAACARTAVASSI"
BASE COUNT          220 a          578 c          521 g          230 t
ORIGIN      
        1 acccgtcgct gaccgtatcg cagatgttca gctccggcga tcggccgaca gcgtggggcg
       61 atgtcgacgg cgacggcctc accgatatga tgttcctcga ggatcaagcg ttctcctatc
      121 atcagggcgg cggcggccag ctcacgctca tcgcgacgac gccgggcatg tacgcggagt
      181 tcctcctcga cgtcgtcgac gccaccggcg acggacgcgg cgatctgttc gacatcgccg
      241 accaggtgaa gttcacgacc tggcccggcg acgggctcgg cgggttcacc aagggcaaga
      301 cgctcatgct ctccgacccg gccgaggtcg gctctgccgt cgacaaggtg ttcgaccggc
      361 gcgggcgcct ggcgatggtg tcgttctacg cgccggtgtg cgatgtctgc acgtcgcggc
      421 ggatcgtcac cctcgtcaat gacggtaccg gcttcacgaa ccccgcgccc ccgcagacct
      481 tcagccagac cgtcaccgac ccggtgttcg ccgacctcga cgacgacgat gtccccgagc
      541 tcgtcatgag caactggtcc ggcggcaaga acgtcgagat ccacgagatg gtcacgcccg
      601 gggtcctcaa cctgcgcggc aagcttccgg ggcaaaagcc gcgcatcggc gacatcgacg
      661 gcgacggccg cgacgacgtg ctcaccagcg agaaggatca tatcaatatc tacctcgcgg
      721 ccaccggtta cacggccgaa ccgacgcccc tggtgttccc cggcatcgac gagccggtcg
      781 tcgcgctcgt cgccgacctc gacggcgacg gaatcgacga cttcgtggtc acggcgctgt
      841 tcggctacga cgccgtgctg gtgcgcagcg gctgcggatg atcgcgtcgg tggtcggatc
      901 gccgaccacg aggccggcgc gccgcggaaa tcgtggcggc cgctcgcgcg tacggccacg
      961 atgtctcgat ccggcctgct cgctcccctc acgctcacgc tcgcgctcgg cgcgtgtacg
     1021 cccacgccga acccggtcgc accgggcacg cacgccggtc ccccgcgacg ctgcgccggg
     1081 cagcccgcgc cggccgtgcc ggtgcaagcg cccgccgcga gcccggctcc gccgctctcc
     1141 ccctgccctt cctgcgcgcc gccacccgct cccgagctcc cggcgcccac ggggacgatc
     1201 gtcgtcactg ccggtgagcg gccgatccgg gtgcatttct cgggcgcgga cgagccctat
     1261 cgcgactgcc aggcgcacgc gtgggtggtg cgcacggtgc ctgccggcgt cgatccggtc
     1321 gccttcgcgg tgcgtgcggt gatcacgggg ccgacgaaat tcgaggccgc gcaggggctg
     1381 cacacgccat tcgccgccgc gagcagcgac ccgggcatcg cgccgctgtc ggcctccggg
     1441 gtgcgcgtga aggtcaagga cggggtggcg accgtcgact tcccgaccag cgcgcggccg
     1501 tacctcgatc aggcagcgtg cgcgcggact gccgtcgcct cgtcgatcc
//
LOCUS       DASWNJ010001416         1508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_95259, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001416 DASWNJ010000000
VERSION     DASWNJ010001416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95259"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1508
                     /locus_tag="VGB85_23560"
     CDS             <1..>1508
                     /locus_tag="VGB85_23560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137089.1"
                     /translation="LHARGQVHGMLTSASVFVGPRGPAVYQYGLAPLCERSVLLRRVR
                     AFSLPNVAPEVLAGGDFTPAADLYAWAVTLAQFAAGTRGAAAIDAARGSQELPGLSPA
                     LRTALQSCLSDDPTARPRDGAELLRLIEIAGLDAAPAAAPRSDESVTNRPIAPEPEPA
                     KPEPARPEPARPETRPSQAPVASAVSAALAASPAPSDDSAHAGSSAPPAATTPATSSV
                     SEPGPPSAGTAAALHPSPPAPIAVPAAAGGAVPAFVPLLPAMKPAAPPRPPTLPPAPP
                     AAHAPVPRPPAPPAKGPPAITPPIPRSLPITSFEERLTADERPRRPATVPPGSMMASP
                     ITPPVTPPNPAPSSPAPAPHPPHPTIPPAVRDVIELMPDDMLNESWRGGKSASNLRRV
                     HVLTDPLNRPTDDSGEGNASQRSALSENVPQAAPQRSPSRGEVIIGGTREELDAASRA
                     TTVRLDPASAAAAVLAATEAAASSSLRVAVSEPPTMRPASGRRTPDPSSSTS"
BASE COUNT          174 a          682 c          493 g          159 t
ORIGIN      
        1 cgctgcacgc ccgcggccag gtccacggca tgctgaccag cgccagcgtg ttcgtgggtc
       61 cccgcggccc ggccgtgtac cagtatgggc tcgcgccgct gtgcgagcgc agcgtgctcc
      121 tgcgccgcgt ccgcgcgttc agcctgccca acgtcgcccc cgaggtgctc gccggcggcg
      181 acttcacgcc cgccgccgac ctctacgcct gggccgtcac gctcgcgcag ttcgccgcgg
      241 gcacgagagg cgcggcggcg atcgacgccg ctcgcggctc ccaggagctg cccggcctgt
      301 caccggccct gcgcaccgcc ctgcagtcgt gcctgtccga cgaccccact gcgcgccccc
      361 gcgacggcgc cgagctgctg cgcctgatcg agatcgccgg cctcgacgcc gccccggccg
      421 ctgccccgcg ttccgacgag agcgtcacga accgaccgat cgcccccgag cccgagcccg
      481 ccaagcccga gcccgccagg cccgagcccg ccaggcccga gacccgcccg tcccaggcgc
      541 ccgtcgcctc cgccgtgtcc gcggcgctcg cggcctcgcc ggcgccctcc gatgacagcg
      601 cccatgcggg gtcctccgcg ccgcctgcgg cgacaacgcc cgcgacttcc tcggtgagcg
      661 agccaggccc gcccagcgcc gggaccgcgg ccgccctgca tccctcgccg ccggctccga
      721 tcgcggtccc ggccgctgca gggggcgccg tgcctgcgtt cgtccccttg ctgccggcga
      781 tgaagccggc cgctccgccg cgcccgccga ccttgccacc agcgcccccc gctgcgcacg
      841 cgccggtgcc ccgtccgccg gccccgcccg cgaagggccc gccggccatc acgccgccga
      901 tcccgcgtag cctgccgatc accagcttcg aggagcgact gaccgccgac gagcgcccgc
      961 gccggccggc cacggtcccg cccggcagca tgatggcgag cccgatcacg cccccggtca
     1021 cgccgcccaa ccctgcgccg tcgagccccg cgcccgcacc ccatccgccc catccgacga
     1081 tccccccggc cgtgcgcgac gtgatcgagc tgatgcccga cgacatgctc aacgagtcgt
     1141 ggcgcggcgg caagtcggcg agcaacctgc gtcgcgtcca cgtgctgacc gacccgctca
     1201 atcgtcccac cgacgacagc ggcgagggca acgcgagcca gcgaagcgca ctgtccgaga
     1261 acgtcccgca ggccgccccg cagcgctcgc cctctcgcgg cgaggtgatc atcggcggga
     1321 cccgcgagga gctcgacgcc gcgagccggg cgacgaccgt gcgcctcgac ccggcctcgg
     1381 ccgccgcggc cgtgctcgcc gcgaccgagg ccgccgcgtc gagctcgctg cgcgtcgctg
     1441 tcagcgagcc gccgaccatg cgcccggcgt ccgggcgccg cacccccgac ccctcgagca
     1501 gcacctcg
//
LOCUS       DASWNJ010001417         1589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_129900, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001417 DASWNJ010000000
VERSION     DASWNJ010001417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129900"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VGB85_23565"
     CDS             complement(<1..189)
                     /locus_tag="VGB85_23565"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY0137090.1"
                     /translation="MTSELPPLRTLLFVPGDRPERMRKALGLGADAQILDLEDSVALE
                     RKAYARDAVAAFVAEAREQ"
     gene            complement(226..408)
                     /locus_tag="VGB85_23570"
     CDS             complement(226..408)
                     /locus_tag="VGB85_23570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137091.1"
                     /translation="MPRREGSMGRDDDIYWRRRAQQERERAMLSDNLTVREVHRELAA
                     AYEARLEFELPRQLAR"
     gene            complement(504..>1589)
                     /gene="ispG"
                     /locus_tag="VGB85_23575"
                     /gene_synonym="gcpE"
     CDS             complement(504..>1589)
                     /gene="ispG"
                     /locus_tag="VGB85_23575"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEY0137092.1"
                     /translation="RQSRQIMVGNVPVGGDAPVTVQTMTNTPTSDPGATISQIRRCEE
                     AGVDIIRVSCPDVESTAALGKIVRAARVPIVADIHFHYKRALEAADAGAACLRINPGN
                     IGSADRVREVVNAAKANGCAIRIGVNAGSLERDLLEKYGEPCPEALVESALDHIKLLQ
                     DHDFHEYKVAVKASDLVLAVAAYQQLAEVVDCPLHLGITEAGGFVGGTVKSAIGMGSL
                     LWYGIGDTIRVSLSAEPEEEVRVGFEILKALGIRNRGVRVVSCPSCARQGFDVIRTVE
                     KLEARLQHIRTPLSLSVLGCVVNGPGEARETDIGLTGGGNGKHMVYLSGITDHTIADE
                     GMVDHIVRLVEAKAAELDAVEAGQVAA"
BASE COUNT          241 a          561 c          525 g          262 t
ORIGIN      
        1 ctgctcgcgc gcctcggcga cgaacgccgc caccgcgtcg cgcgcatagg ctttgcgctc
       61 aagcgcgacc gagtcttcga gatcgaggat ctgcgcatcg gcgccgaggc cgagcgcctt
      121 gcgcatgcgc tcgggccggt cgccggggac gaacaacagc gtgcgcagcg gtggtagctc
      181 ggaggtcatg cccgccctta cccgcgcagg gcggcgccgg gcaactcagc gggcgagttg
      241 cctcggcagc tcgaattcca gccgcgcctc atacgccgcg gcgagctcgc gatgcacctc
      301 gcgcaccgtc agattgtcgc tcagcatcgc gcgctcgcgc tcctgctgcg cgcggcgacg
      361 ccagtatata tcgtcatccc tacccatgct gccctcccgc cgtggcatcg actcgtgacg
      421 taacgatcct tacgccgcgc atgcggtaag caacgattaa ctggatcaag cgccttttca
      481 gaagcttagg gtgagtgcgc ggctcaggcc gcgacttgtc ccgcctccac cgcatcgagt
      541 tccgccgcct tggcctcgac caggcggacg atgtggtcga ccatcccctc gtcggcgatg
      601 gtgtggtcgg tgatgcccga cagatagacc atgtgcttgc cgtttccgcc gccggtaagg
      661 ccgatgtcgg tctcgcgcgc ctctccggga ccgttgacga cgcagccgag caccgagagc
      721 gacagcggcg tacggatgtg ctgcagccgc gcctcgagct tctcgacggt gcggatcacg
      781 tcgaagccct ggcgcgcgca cgacgggcac gacaccacgc gcacaccgcg gttgcggatg
      841 ccgagcgcct tcaggatctc gaacccgacg cggacttcct cctcgggctc ggccgaaagg
      901 ctgacacgga tcgtgtcgcc gatcccgtac cacagcagcg atcccatgcc gatcgcgctc
      961 ttgaccgtgc ccccgacgaa gcccccagcc tcggtaatgc cgaggtggag cggacaatcg
     1021 acgacctcgg cgagctgctg ataggcggcg accgcgagga ccaggtcgga cgccttcacc
     1081 gccaccttgt actcgtggaa atcatggtcc tgcagcagct tgatatggtc gagcgcgctc
     1141 tcgaccagcg cctcggggca gggctcgccg tatttctcga gcaggtcgcg ctccaggctg
     1201 ccggcgttga cgccgatgcg gatcgcgcag ccattggcct tggcggcgtt caccacctcg
     1261 cgcacgcggt cggccgagcc gatgttgccg gggttgatgc gcaggcacgc cgcgcccgca
     1321 tccgccgcct ccagcgcgcg cttgtaatga aaatggatgt cggcgacgat cggcacccgc
     1381 gccgcgcgca cgatcttgcc gagcgcggcg gtgctctcga cgtcggggca ggacacgcgg
     1441 atgatgtcga ccccagcctc ctcgcagcgg cggatctggc tgatcgtcgc gccggggtcg
     1501 ctcgtgggcg tattggtcat cgtctgcacg gtgacgggcg cgtcgccgcc gacgggaacg
     1561 ttgccgacca tgatctgccg ggattgccg
//
LOCUS       DASWNJ010001418         1787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_26058, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001418 DASWNJ010000000
VERSION     DASWNJ010001418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26058"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VGB85_23580"
     CDS             complement(<1..740)
                     /locus_tag="VGB85_23580"
                     /inference="COORDINATES: protein motif:HMM:NF020916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HEY0137093.1"
                     /translation="MSAATANQIDRREFKYLIDASTAARVRAAIRPFCDIDPWAAKNP
                     RGVYTIESIYLDNPGLSLFWANEHEQVDRIKVRVRGYAGVPGSPVFFEVKRRINDVIS
                     KSRGKVPRAQYMELLTNPAAKIPDSIGPKDRNAVERFLSIQRTMQLRPFTMVRYEREP
                     YFSRIDDYARVTFDTAIRAQATDHYSFDLPARGWRALDDAVTQRTLESLVVLELKFTA
                     AVPLWLVNIVERLNLTRRSFSKYGTSIRA"
     gene            1075..>1787
                     /locus_tag="VGB85_23585"
     CDS             1075..>1787
                     /locus_tag="VGB85_23585"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEY0137094.1"
                     /translation="MAHRRYKWAIRLVGPVLLVFVLLRLQDSGAVLDALRLADAAPIV
                     AAMLLNAVNYYFKVLRWDVLLEARGYQYSRLRAWTSFLSSGYVGLMTPGRVGDLLRTQ
                     YLRHDLGMPYADGIALLAVDRLCDIYVLLAFAGVGLAFFGAALTGDLALVLWVGVAAT
                     ALGPLLLFKPGLLRRAAAMIYKKMPGEPDIAGFDRFLAGLRHQKLHHIARAVFWTVLA
                     FGINYLQGWMLAHALHIDLG"
BASE COUNT          266 a          640 c          577 g          304 t
ORIGIN      
        1 gcgcggatcg acgtcccgta cttcgagaac gagcgccgcg tcaggttgag ccgctcgacg
       61 atgttgacca gccacagcgg caccgcggcg gtgaacttga gctcgagcac gacgagcgac
      121 tcgagcgtgc gctgcgtgac cgcgtcatcg agcgcacgcc agccgcgggc cggcaggtcg
      181 aacgagtagt ggtcggtcgc ctgggcccgg atcgcggtgt cgaaggtgac gcgcgcgtag
      241 tcgtcgatgc ggctgaagta gggctcgcgc tcgtagcgga ccatcgtgaa cggccgcagc
      301 tgcatcgtgc gctggatcga caggaagcgc tcgaccgcgt tgcggtcctt gggcccgatg
      361 ctgtcgggga tcttggccgc cgggttggtc agcagctcca tgtactgcgc gcgcggaacc
      421 ttgcctcgcg acttcgagat gacatcgttg atccggcgct tgacctcgaa gaacaccggg
      481 ctgcccggca cgcccgcata gccgcgcacg cgcaccttga tgcggtcgac ctgctcgtgc
      541 tcgttggccc agaacagcga caggcccggg ttgtcgaggt agatgctctc gatcgtgtag
      601 accccacgcg ggttcttggc cgcccacggg tcgatgtcac aaaacggacg gatcgcagca
      661 cgcaccctcg ccgccgtcga ggcgtcgatc aggtacttga attctcgccg gtcgatctgg
      721 ttcgccgtgg cagccgacat gggaccccga tgaagacgtg taaaaacaac gatgtggcgc
      781 gccgttgacc ccggccgcca cggccgagca tggtactcgc agtgacagcc ttgttacagc
      841 cctgtcaccg ccccgcgcaa actatgggcc gcgcggtgtc agcggtcctg gcgcccgaac
      901 cgggccagga cgcccggaca gccctcgaca cacccgaccc ggcgcggatc cggctcgaat
      961 gcgcgagcag gagatcgcgg atcctgctgg cgagaacttt tcaagagctc gccgccgtgg
     1021 atcccggcgt gcatcgctcc cgcgcatcgc cgcggaccca cgtatactcc cgccgtggcc
     1081 cacaggcgct acaagtgggc gatccgcctg gtcggcccgg tgctgctggt gttcgtgctc
     1141 ctgcgcctgc aggactccgg cgccgtgctc gacgcgctgc gcctcgccga cgccgcgccg
     1201 atcgtcgccg cgatgctcct caacgcggtc aactactact tcaaggtcct gcgctgggac
     1261 gtattgctcg aagcccgcgg ctatcagtac agccgtctac gcgcgtggac ctccttcctc
     1321 tcgtccggct acgtcggcct gatgaccccc ggccgcgtcg gcgacctgct gcgcacccag
     1381 tacctgcgcc acgacctcgg catgccctac gccgacggca tcgccctgct cgcggtcgac
     1441 cgcctgtgtg acatctacgt cctgctcgcg ttcgccggcg tcggcctcgc cttcttcggc
     1501 gccgcgctga ccggtgacct cgcgctcgtg ttgtgggtcg gcgtcgccgc gaccgcgctc
     1561 ggacccctgc tgctgttcaa gccgggcctt ttgcgccgcg ccgcggccat gatctacaaa
     1621 aaaatgccgg gcgagcccga catcgccggc ttcgaccgct tcctcgccgg gctcaggcac
     1681 cagaagctgc accacatcgc gcgcgcagtg ttctggacgg tgctcgcgtt cggcatcaac
     1741 tacctccagg gctggatgct ggcccacgcg ctgcacatcg acctcgg
//
LOCUS       DASWNJ010001419         3921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_236595, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001419 DASWNJ010000000
VERSION     DASWNJ010001419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236595"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VGB85_23590"
     CDS             <1..378
                     /locus_tag="VGB85_23590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137095.1"
                     /translation="HENHAYQRVYWGGAAIALIADLALREAGSTLDAAMRHIQRCCAD
                     TQRVWPGEQVLRAMNEAGPAMATARLDLEALSGPILRSAEFPELSAIYRKLGVVVVDG
                     DPTLDESAPLAKIRRAIFTQPAP"
     gene            518..787
                     /locus_tag="VGB85_23595"
     CDS             518..787
                     /locus_tag="VGB85_23595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137096.1"
                     /translation="MNVKHIAATLAILGSSSFALVGCNKNKEGTETPAGDTAKPGEGS
                     CGEKKDGSCDANKDAPGGDAPADAPGGDPPAGGETAPAAAPPAAN"
     gene            917..1786
                     /locus_tag="VGB85_23600"
     CDS             917..1786
                     /locus_tag="VGB85_23600"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEY0137097.1"
                     /translation="MRSSDDIPTGVGLGLRGVFAAKIDAGEADGRVPFFEISPENHMH
                     RGGAGPARLARIAQRFPILTHGLAMSLGGLEPHDPEYFAQLRTFLDRVDATRTRWHSD
                     HLCFCGVDGKALHDLLPVPFTAASARRIAGRVREAADRLERPMAVENISYYVPLGKPA
                     LDEPEFITELLGLADCSLLLDVNNVFVNARNHGFDPQAWLEKIPLERVVQMHVAGHEL
                     WDDSDSLIIDTHGAPVRSEVDALLAWVVERTGPVPVLFEWDSNIPGLPALLAERDRLQ
                     TVYDAALARRSAP"
     gene            1783..2613
                     /locus_tag="VGB85_23605"
     CDS             1783..2613
                     /locus_tag="VGB85_23605"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HEY0137098.1"
                     /translation="MTAAPDPKASSDEAVLRAFDAAMCELDAAARIAADLPGWLAERG
                     VAPDDREVLTQHRARLLVYRTMVHSRLRGVVEEYMPRTAAEIGKPRLRAEIAAFVAEQ
                     APRTVYFRDIPGEFLAWAAPRWRADPGLAPYLADLATHEWLEGEVANTPLGGEPASEL
                     PLALDRPVQLDGSACVRRYGFAVHRPASDPPVPPAPEPTAILAYRDRDTERVRLLELT
                     PRAAALCTRLLAGEALQGALAGACSDIGEVMNDEYLAAMAGFLADLGERGVLLGARAD
                     "
     gene            2707..3057
                     /locus_tag="VGB85_23610"
     CDS             2707..3057
                     /locus_tag="VGB85_23610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137099.1"
                     /translation="MLKELGSRWRTMLALLIAVATALMFSTATPATPPTTREGEAFCT
                     PELHDTPFISKQESGERTAPRGETHALAAPRIGGLTFMELQAVGVGVFPALHFPVRTR
                     SPGGLYPRGPPLTA"
     gene            3314..>3921
                     /locus_tag="VGB85_23615"
     CDS             3314..>3921
                     /locus_tag="VGB85_23615"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEY0137100.1"
                     /translation="MFGKLSGKVWSIQFAVSLALSACSGAKGTAPEPAPEAQPQVLAG
                     DNAGEVRLSPASMAYVKVEAVNLEPEHAVIRAPARVAFRDGAVSKVGAPIPGRVMKLH
                     VEAGAQVKVGDPLVTIASPEASGFHMDLARARIELTAAKDNYERQSQMVAKGVGRDRG
                     MEAGRQIAGYILQQAAAGDVGERLDLASPDQRQQGAHVNSGGL"
BASE COUNT          561 a         1440 c         1340 g          580 t
ORIGIN      
        1 cacgaaaatc atgcgtatca gcgcgtctac tggggtgggg ccgcgatcgc gctgatcgcc
       61 gacctcgctc tgcgcgaggc cggcagcacg ctcgacgcgg cgatgcggca catacaacgg
      121 tgttgcgccg acacgcagcg ggtctggccg ggagagcagg tcctccgggc catgaacgag
      181 gcaggccctg cgatggccac tgcacgcctc gatctcgagg ccctgagcgg tcctatcttg
      241 cgcagcgccg agttccccga gttgtccgcg atctatagga agctcggagt ggttgtggta
      301 gacggggatc caacgctgga tgagtccgct cccctcgcca aaatccgtcg cgcgatcttc
      361 acgcagcccg caccgtaacc gcgggcctgt gagcgtcgtt cgtcggaggt tcgggcgcgc
      421 ggccccctgg ccactcgact aaacgggcac atccgggtcg ccacggcccg gacgagcgcg
      481 tcacgtctca ccaagcacag acaacggagt aatcatcatg aacgtcaagc acatcgctgc
      541 caccctcgcc atcctcggtt cgtcctcgtt cgccctcgtc ggctgcaaca agaacaagga
      601 aggcaccgag acccccgccg gtgacaccgc caagcccggt gagggctcct gcggcgagaa
      661 gaaggacggc agctgcgatg ccaacaagga tgcccccggt ggcgacgccc cggccgacgc
      721 tcccggtggc gatcccccgg ccggtggcga gaccgccccg gctgccgccc ccccggccgc
      781 caactgagtc ggatggaccg ctcctggcct tcgggccagg tggtccgcgc tgactggccc
      841 ctcggggtcg acggtcagga tgctcctcac ggggaggtcc tggccgttgt tacgttgtag
      901 aatcccggct cctcgcatgc gctcctccga cgacatccct accggcgtgg gcctcggcct
      961 gcgtggcgtc ttcgcggcga agatcgacgc gggcgaggcc gacggtcgcg tccccttctt
     1021 cgagatctcg ccggagaacc acatgcaccg cggcggcgcc ggaccggccc gcctcgcgcg
     1081 catcgcccag cgcttcccga tcctcaccca cggcctggcg atgtcgctcg gcggcctcga
     1141 gccgcacgac cccgagtact tcgcgcagct gcggaccttc ctcgatcgcg tcgacgcgac
     1201 ccggacccgc tggcactccg accacctgtg cttctgcggc gtcgacggca aggccctgca
     1261 cgacctgctg ccggtgccgt tcaccgcggc ctcggcgcgg cgcatcgccg gccgcgtgcg
     1321 cgaggcggcg gaccgcctcg agcggccgat ggcggtcgag aacatcagct attacgtccc
     1381 gctcggcaag ccggccctcg acgagcccga gttcatcacg gagctgctcg gcctcgcaga
     1441 ttgctcgctg ttgctcgacg tcaacaacgt cttcgtcaac gcgcggaacc acggcttcga
     1501 cccacaggcc tggctcgaga agatccccct cgagcgggtc gtacagatgc acgtcgccgg
     1561 ccacgagctc tgggacgaca gcgactccct catcatcgac acccacggcg cgcccgtacg
     1621 cagcgaggtc gacgcgctgc tcgcctgggt cgtcgagcgc accggccccg tgccggtgct
     1681 gttcgagtgg gacagcaaca tcccgggcct gcccgccctg ctcgccgagc gcgaccgcct
     1741 gcaaaccgtc tacgacgcgg ccctggcccg ccggagcgcg ccatgaccgc ggcgcctgac
     1801 ccgaaggcca gctccgacga ggccgtgctg cgggccttcg acgcggcgat gtgtgagctc
     1861 gacgcggcgg ctcgtatcgc ggcagatctc ccgggctggc tcgccgagcg cggcgtggcg
     1921 ccggacgacc gcgaggtgct cacgcagcac cgcgcgcgcc tgctcgtcta tcgcacgatg
     1981 gtccacagcc gcctgcgtgg ggtcgtcgag gagtacatgc cgcgcacggc cgccgagatc
     2041 ggcaagcccc gcctgcgcgc cgagatcgcg gccttcgtcg ccgagcaggc gccgcgcacc
     2101 gtctacttcc gcgacatccc gggcgagttc ctggcctggg ccgcgccccg ctggcgcgcc
     2161 gaccccgggc tcgcgccgta cctggccgac ctggcgaccc acgagtggct cgagggcgag
     2221 gtcgccaaca ccccgctggg cggcgagccc gcgagcgagc tcccgctggc gctcgatcgt
     2281 ccggtccagc tcgacggcag cgcctgcgtg cgtcgctacg ggttcgccgt gcaccgcccc
     2341 gcgagcgacc cgcccgtgcc gcccgcgccc gagccgaccg cgatcctcgc ctaccgcgac
     2401 cgtgacacgg agcgcgtccg tttgctcgag ctgaccccgc gagcggccgc cctgtgcacg
     2461 cgactgctcg ccggcgaggc cctgcagggc gcgctcgcgg gcgcgtgctc ggacatcggc
     2521 gaggtgatga acgacgagta cctcgcggcg atggccggct tcctcgcgga cctcggcgag
     2581 cgcggcgtgc tgctgggagc gcgcgcggac tgatgtccgc tcgttggaga caacaaagct
     2641 cacgtttttt cgcggatcgc tcgatgggtg accacgactt cgtgtgatac ggtcgctttg
     2701 ttccaaatgc tgaaggaact cgggtcgaga tggagaacga tgctggcgct gctgatcgca
     2761 gtcgccaccg cattgatgtt ctccactgcg accccggcga ccccgccgac gacccgcgag
     2821 ggcgaggcct tctgcacccc cgagctgcac gacacgccct tcatctccaa gcaggagtcg
     2881 ggtgagcgca cggcgccgcg tggcgagacc cacgcgctcg ccgctccgcg gatcggcggg
     2941 ctcacgttca tggagctcca ggccgtcggc gtcggggtgt tcccggcgct gcacttcccg
     3001 gtccgcacga ggtctcccgg gggtctgtat cctcgtggcc ccccgctgac cgcctgatcc
     3061 actccgacac ctgtcgatcg tgcgccgtga aggcgtcggt ccgcggggct cgggagcgtg
     3121 ttgcgtgcgt cggccagatc gctggcctct tacaactgca cgacgatggg attcgaccgg
     3181 ccccgggatg gtggcagcgg cgtgtcctgg tttcgtgttg cccgagatcc gacccaggcc
     3241 agcgccggga cgcggacacg gggtttcgtc ggtcgaagtc gcgccatccg ggcgcgtgtg
     3301 gggagcgttt gcgatgttcg ggaaattatc gggcaaggtg tggagcattc agttcgcggt
     3361 gagcctcgcg ctcagcgcct gcagcggggc caagggcacc gcgccggagc cggcccccga
     3421 ggcccagccc caggtcctcg ccggggacaa cgcgggcgag gtgcgcctga gcccggcctc
     3481 gatggcctat gtcaaggtcg aggccgtcaa cctcgagccg gagcacgcgg tcatccgcgc
     3541 gccggcgcgg gtcgccttcc gcgacggcgc cgtgtccaag gtcggcgcgc cgatccccgg
     3601 ccgcgtcatg aagctgcacg tcgaggccgg cgcccaggtc aaggtcggcg acccgttggt
     3661 gacgatcgcc agccccgagg cgtccggctt ccacatggac ctcgcgcggg cccgcatcga
     3721 gctgaccgcg gccaaggaca actacgagcg ccagtcacag atggtcgcca agggcgtcgg
     3781 ccgcgaccga ggcatggagg ccggccggca gattgcgggg tacattctcc agcaggccgc
     3841 cgccggtgat gtgggcgagc gccttgatct tgccagcccg gatcagcggc agcagggcgc
     3901 gcacgtaaat tcgggtgggc t
//
LOCUS       DASWNJ010001420         1850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_662023, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001420 DASWNJ010000000
VERSION     DASWNJ010001420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662023"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1850)
                     /locus_tag="VGB85_23620"
     CDS             complement(<1..>1850)
                     /locus_tag="VGB85_23620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137101.1"
                     /translation="VVHRWDGASPFAAAAADLDGDGARELYVSTGTDLEIIELLPGAA
                     GPWTARTVYSARDTLESGVHVLRPIDLDGDGRQELAAGLQGWRAFDVRVLARDRGDAA
                     LRTVARDKLGTIVAVAGLRRDGQAPLLVATNHHHGTSNLVFPPDRPAGEPEGLYLYTL
                     DDGRLTRRGHLPLRLPPIASSGGSRLVGDIDGDGRDDLASDFGTDDELHALIHLQRPS
                     GALAPVILGHVEPLALAQLDDDRADELIANVRDADGVPRVHVLGVGDDVLAPVEPPTV
                     ATTALTDPDDDAWTRQWAQATTIHGLGLLADAAAAYQDLGRRSARPGLRAAAYLHAAA
                     LREHLGEDRAALPLFIGAAQGPGLASEALQGALRTQLRLGAYEEADASLAAVLALPEL
                     PATTRDELARRWAPLIAAAGERVDLDFSRPLAQAWSLHDPLALRRIPGASGLGIDTTR
                     AGVLASLPVEWSGDLLELTADFTIDTTEWSSRFEVALVPEDPALADVAELEISTFGTS
                     RGGVAVRRSSSCMLGRHWLHDRSERDPVATTGVSTRQVMRISAIPALGEAGCEIREAG
                     EPERVVHEPLLRPLPAGGRHRLVVRLHNDVPAHVSAQLHRITLRGAHVVD"
BASE COUNT          215 a          617 c          798 g          220 t
ORIGIN      
        1 gtcgacgacg tgtgcgccgc gcagggtgat gcgatgcagc tgcgcggaca cgtgggcggg
       61 gacgtcgttg tgcaggcgga cgacgaggcg gtggcgaccg ccggccggga gggggcgtag
      121 caggggctcg tgaacgacgc gctcgggctc gccggcctcg cggatctcgc agccggcctc
      181 gcccagcgcc gggatcgccg agatgcgcat cacctgtcgc gtggacacgc cggtggtcgc
      241 gaccgggtcg cgctcgctgc ggtcgtgcag ccagtggcgc ccgagcatgc agctcgacga
      301 ccggcggacc gcgaccccgc cgcggctggt gccgaaggtg ctgatctcga gctccgcgac
      361 atccgcgagc gccggatcct cggggacgag cgcgacctcg aagcggctgc tccactcggt
      421 cgtgtcgatc gtgaagtcgg cggtgagctc gagcaggtcg ccggaccact cgaccggcag
      481 cgaggcgagc acgccggcgc gggtggtgtc gatccccagg ccgctggcgc cggggatccg
      541 ccgcagggcg agcgggtcgt gcagcgacca cgcttgcgcg agcgggcgcg agaagtcgag
      601 gtcgacgcgc tcgccggccg cggcgatcag cggcgcccag cggcgcgcga gctcgtcgcg
      661 cgtggtggcc ggcagctcgg ggagcgcgag cacggccgcg aggctggcgt cggcctcctc
      721 gtaggcgccg aggcgcagct gcgtgcgcag ggcgccctgc agcgcctcgg aggcgagccc
      781 cgggccctgg gcggcgccga tgaacagcgg cagcgcggcc cggtcctcac cgaggtgctc
      841 gcgcagcgcg gcggcgtgca ggtaggcggc ggcgcgcagg ccgggccggg cgctgcgacg
      901 tccgaggtcc tggtaggcgg cggcggcgtc ggcgagcagg ccgaggccgt ggatcgtggt
      961 cgcctgggcc cactggcggg tccaggcgtc gtcgtcgggg tcggtgaggg cggtggtggc
     1021 gacggtcggc ggctcgacgg gcgcgagcac gtcgtcgccg acgccgagga cgtggacccg
     1081 ggggacaccg tcggcgtcgc ggacgttggc gatcagctcg tccgcgcggt catcgtcgag
     1141 ctgggcgagc gcgaggggct cgacgtggcc gaggatcacc ggggcgagcg cgccggaggg
     1201 tcgttgcagg tggatcaggg cgtgcagctc gtcgtcggtc ccgaagtcgc tggcgaggtc
     1261 gtcgcggccg tcgccgtcga tgtcgccgac gagccgactg ccgcctgagc tggcgatcgg
     1321 cggcaggcgc agcggcaagt ggccgcgccg ggtcaggcgg ccgtcgtcga gggtgtacag
     1381 atagagcccc tcgggctcgc cggcggggcg gtcgggcgga aacacgaggt tgctggtgcc
     1441 gtggtgatgg ttggtggcga ccagcagcgg ggcctggccg tcgcggcgca ggccggcgac
     1501 ggcgacgatc gtgccgagct tgtcgcgggc gacggtccgc agcgccgcgt cgccgcggtc
     1561 gcgggcgagc acgcggacgt cgaaggcgcg ccagccctgg aggccggcgg cgagctcctg
     1621 acgcccgtcc ccgtcgaggt cgatcgggcg caggacgtgg accccggact cgagggtgtc
     1681 gcgcgcgctg tagacggtgc gcgccgtcca cgggccggcg gcgccgggga gcagctcgat
     1741 gatctcgagg tcggtgccgg tcgagacgta cagctcgcgc gcgccgtcgc cgtcgaggtc
     1801 ggcggcggcg gccgcgaacg ggctggcgcc gtcccagcgg tgcacgaccg
//
LOCUS       DASWNJ010001421         9244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_95345, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001421 DASWNJ010000000
VERSION     DASWNJ010001421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 9244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9244
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95345"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VGB85_23625"
     CDS             <1..205
                     /locus_tag="VGB85_23625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3556 domain-containing protein"
                     /protein_id="HEY0137102.1"
                     /translation="GHLHNEDLLRAVQAQCEFTSSELRCIMVESQPLGGAGLDWRISD
                     AHDGLLGRGTTSVAELRARAPWG"
     gene            329..1828
                     /locus_tag="VGB85_23630"
     CDS             329..1828
                     /locus_tag="VGB85_23630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137103.1"
                     /translation="MTPLRRTILATFASLVLHGLTVARLSCGFNVDIEFPELEYDITE
                     MDFVDPEKELGKTQEPPPPEPEVVPAPMGPDLPPEGEGPKPEEKPPEPPKPKVFGEKT
                     TKVDELGPANSNFYMLLNAKKVAGLPFAESVVEIMAPLPDFQLIIAGGGFHALRDFNY
                     LVIASPNLRDLTQTFLAVEYKLSQEEMIAGIERAVAADGEQIEWVEKDGRKMGNPHPI
                     GAPDKDRDPRWFVFLDDKVAVYVREEFLPAIARGAEDGKKTSGNFVANLAKMKTFAAR
                     EPRAGLQLVLKDILASIKVKKSPFEIPDSVELMAEAKASPELVVKMEFVDEVAAKRME
                     NQWRDDLPRFIDEKVPFIVRGMVRGFYDDAEFTLAGKEIILRSNFTESQASLILDQIA
                     AGSRKMLRKTPEELEAARKAREEIWQLRKNGKLTPSQALEKQKAGSSGIAPAGTPKPA
                     DSKTPEPLDKPAEPTPEPSGEPELKPTEPKPESPAEAPVDAKPPEPTPP"
     gene            complement(1835..2155)
                     /locus_tag="VGB85_23635"
     CDS             complement(1835..2155)
                     /locus_tag="VGB85_23635"
                     /inference="COORDINATES: protein motif:HMM:NF042704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-phosphate processing receiver
                     domain-containing protein"
                     /protein_id="HEY0137104.1"
                     /translation="MRVFLDDERMPEEVPGLPDPDAWVIVRSAAECIALLAAGGVEAV
                     SLDHDLGADVPTGYDVACWIAAAQVEGGPLPAQVYVHTANPVGRGRIVAALRGLAVTV
                     LTGG"
     gene            complement(2393..2956)
                     /locus_tag="VGB85_23640"
     CDS             complement(2393..2956)
                     /locus_tag="VGB85_23640"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY0137105.1"
                     /translation="MTVRGRVIDVETGEGVPGMQVRMTPKGQQRVYSSQEPMAAGEVT
                     DVQGRFELERVSAGAVQVMIMPRAMVENGYEFTGFGLEIAGPGPVVELAPMKLTRLRI
                     KDGEQAGDLGLTTGKELTITAVRAGGPAAQAGIVVGDQVVRVDGHPVTGGEMRLFYSL
                     SRVPAGTRLVLGLARGVDVEVVAGEPR"
     gene            complement(2953..4920)
                     /locus_tag="VGB85_23645"
     CDS             complement(2953..4920)
                     /locus_tag="VGB85_23645"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0137106.1"
                     /translation="MLIGVLGSLLWWLSSRTDAPETAEATVVVESMATGERVGTPDVP
                     RVVQQASLSGRVRDEQGRGISGASVCVAGFSRTLDRRDAEDVRCVQSEADGGYHITAL
                     WPVAYFVRASAPHFIPGQHRRGTRARTLVALRAGQEARDIDVVLQGGGVELRGVVHDL
                     SGGVLEGAQVQAGDADARSGPDGTFLLWVAPGQVYVTARAAGYAQGSAPGRAPGQWFE
                     LRLTPESVLVGRVVRAGDRAPLAGASVYAQASDAWNPVGPAITDASGNFRVEGLLPGT
                     YKLEAKHDEAYGMAPESAVLGLGETSEVIEIAAHPAHVVAGTVVRAGGDPCAGGYLRL
                     TEAGRSSRASGIDLDGSVYVQGVLPGTYGVKLECAGAVALPEYPQVVVSDASVSGLRW
                     EVQRGQAIRGQVLAGGAGVRDIWLHAEPQIDPQAPRAHTTSEVRAVTLADGSFELAGL
                     LAGRYRLRANSDEYPTAAVEVALLDGQDLEGVRIALPATGKLRGTVKDESGEPVKGVE
                     VHVRGDGPDGLRTSTVDDGSFRFAHLPAGAYRVTARADHRALRAPGATEDLEGVAAEV
                     VVGAVREVRLVVESRRGRITGRVVDSAGGPLLDAFVEPAREPDGPAMPGVLQGARMDA
                     FFGDVGGRRLTDQDGRFELGGLGEGKYSARV"
     gene            complement(4955..5641)
                     /locus_tag="VGB85_23650"
     CDS             complement(4955..5641)
                     /locus_tag="VGB85_23650"
                     /inference="COORDINATES: protein motif:HMM:NF016932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEY0137107.1"
                     /translation="MTDGADGQDAPGWAAIDDALARLYVGQVPHQFASQRAYEPDAES
                     PLPAIAVYEARRPDSWHFVTYGLSELFDKSSADPDVSGFGFELTMRVPRAADETVPPT
                     WPLRALQGLGRYVLGTGQGFDTGHCADLGGSIAPGIESALTCIACVPDPLLRKIYTPF
                     GSVLFLQIVGVTRDELDAMQRLDHEGVVHLLGELDFHGISDPARSSYFTDPNKAPVLR
                     RYQLGVLLDR"
     gene            complement(5647..6789)
                     /gene="serC"
                     /locus_tag="VGB85_23655"
     CDS             complement(5647..6789)
                     /gene="serC"
                     /locus_tag="VGB85_23655"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEY0137108.1"
                     /translation="MASEKPKLWNFSAGPAILPPVVFERAAEAVRELRADGHAPDAKG
                     VGMSILEISHRSKPYEAVTFGAEKLCHEVLGIPETHQVLFLQGGASLQFAMLAMNLRE
                     AGKPAAYVDTGVWSQKAIIESQGLGETTVVASSEATNYDRIPAFPAADTYAGASYLHI
                     TTNNTIFGTEYPEIPEAQGGVPLVIDFSSHAGSRPMALGNAAFGYAGAQKNLGPSGVT
                     LVYIRKDLLNKKPAAHVPVILRYATHAKEPSLYNTPNTFGVLVLKLVLEWMRDAGGLT
                     EIGAVNERKANKLYAALERSSVFRPHAQPGSRSRMNVTWTANGAPEAEREALTSRFIK
                     EAQAAGFDGLKGHRLVGGFRASIYNAFPEAGVDALCEFMAEFERRL"
     gene            complement(6857..8287)
                     /locus_tag="VGB85_23660"
     CDS             complement(6857..8287)
                     /locus_tag="VGB85_23660"
                     /inference="COORDINATES: protein motif:HMM:NF015376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HEY0137109.1"
                     /translation="MPAQRPHTRLPGRTDRLLGVLGLWLALLLTGAFSLSQVPEAAAA
                     SSKKKTSKKKKSKKKSRKRPVASKPAPVLPVSREVDNTWGVTSGGEILEDEAEGDAEE
                     DADTDDSTPQSELDAIERELLANPPAPEMVKIELPPVVSDPRPIDRKPGSVKHEVIPG
                     ETLEEIAERYKVSRDDIIRWNHLDKKKPKLPLPGKTLRIVTADAPLPRERVEHVAKRG
                     ETFESVAKEYGVEVEKLKRWNKKAGKKVVAKKKLVVWREAPPLPPPLSGTGLAAKLQQ
                     LRVPSGGISIGKPSRGRLVRGVPLPERPDLYVRRKPDESYGSSHAIAELMAAIATFRH
                     QTGFGRSIVIGGISRARGGRFRPHKSHQSGRDIDIRMPLTAAAEGKRHITANDIDWKA
                     TWQLMRTFIDRGQIEYIFLDFGLQKRIYKVAREQGATKEQLEHWLQWPVRKPKHQIIR
                     HVEGHKVHFHVRVRCADNEKGCVTAR"
     gene            complement(8655..>9244)
                     /locus_tag="VGB85_23665"
     CDS             complement(8655..>9244)
                     /locus_tag="VGB85_23665"
                     /inference="COORDINATES: protein motif:HMM:NF016218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEY0137110.1"
                     /translation="ERKAKSATGLARPDFYDWESEAKKQVEAKTPDLVVVIMGGNDGQ
                     DLTPKSGKGKRVAWKSDGWNEAYKTRVSDFLKEVGVDQRKVLWLGLPKSEINSFEQKL
                     TIIRQVQSEAIAELGEAGTYLDTTPFLTDAKGVLLDKATVGKRKDQDLREDDGIHFTM
                     AGSEYFADKVYPEVLRVLGVADAEPEAPSKKKKAK"
BASE COUNT         1325 a         3559 c         2950 g         1410 t
ORIGIN      
        1 cggccacctg cacaacgagg acctgctgcg cgcggtccag gcgcagtgcg agttcacgag
       61 cagcgaattg cgctgcatca tggtcgagtc ccagccgctc ggcggcgccg gcctcgactg
      121 gcggatctcc gacgcccacg acggtctgct ggggcgcggc accacctcgg tcgccgagct
      181 gcgtgcgcgg gccccctggg gctgaaaacc ccggccaccc gccatccttc gccgccccac
      241 gcccacccgc gagcgcctcc ccgcccggcg ttcgcgggcg aacgggcacg gcccgccggc
      301 cgtgctatct ttggcgccgc ccgccgccgt gaccccgctg cgcagaacca tcctggcgac
      361 cttcgcctcc ctcgtcttgc acgggttgac cgtcgcgcgc ctcagctgcg ggttcaacgt
      421 cgacatcgag ttcccggagc tcgagtacga catcacggag atggacttcg tcgaccccga
      481 gaaggagctc ggcaagaccc aggagccgcc gccgcccgag cccgaggtcg tgcccgcgcc
      541 gatgggcccc gacctgccgc cggagggtga gggtcccaag ccggaggaga agccgccgga
      601 gccgcccaag cccaaggtgt tcggcgagaa gaccaccaag gtcgacgagc tcggcccggc
      661 caacagcaac ttctacatgt tgctcaacgc caagaaggtc gcgggactgc cgttcgccga
      721 gagcgtcgtc gagatcatgg cgccgctgcc ggacttccag ttgatcatcg cgggcggcgg
      781 ctttcatgcc ctgcgcgact tcaactacct cgtgatcgcc agcccgaacc tgcgcgacct
      841 gacgcagacc tttttggcgg tcgagtacaa gctctcgcag gaggagatga tcgccggcat
      901 cgagcgcgcc gtcgcggccg acggcgagca gatcgagtgg gtcgagaagg acgggcgcaa
      961 gatgggcaac ccgcacccga tcggcgcccc cgacaaggac cgtgacccgc ggtggttcgt
     1021 gttcctcgac gacaaggtcg ccgtctacgt gcgcgaggag ttcctgccgg cgatcgcccg
     1081 cggcgccgag gacggcaaga agacctccgg caacttcgtc gccaacctcg ccaagatgaa
     1141 gaccttcgcc gcccgcgagc cacgcgcggg cctccagctg gtcctcaagg acatcctcgc
     1201 cagcatcaag gtcaagaaga gcccgttcga gatccccgac agcgtcgagc tcatggccga
     1261 ggccaaggcc tcgccggagc tggtggtcaa gatggagttc gtcgacgagg tcgccgccaa
     1321 gcgcatggag aaccagtgga gggacgacct cccgcgcttc atcgacgaga aggtcccctt
     1381 catcgtgcgc ggcatggtcc gcggcttcta cgacgacgcc gagttcaccc tcgcaggcaa
     1441 ggagatcatc ctgcgcagca acttcaccga gagccaggcg agcctgatcc tcgaccagat
     1501 cgccgccggc tcgcgcaaaa tgctacgcaa gacccccgag gagctcgagg ccgcccgcaa
     1561 ggcccgcgag gagatctggc agctgcgcaa gaacggcaag ctgaccccct cccaggccct
     1621 cgagaagcaa aaagccggct cctcgggcat cgcccccgcc ggcaccccga agcccgccga
     1681 cagcaagacc cccgagccgc tggacaagcc cgccgagccc acgcccgagc cgtccggcga
     1741 gcccgagctc aagcccaccg agcccaagcc cgagtcgccc gccgaggccc cggtcgacgc
     1801 caagcctccc gagccgaccc ccccgtgaat cgggtcagcc gcccgtcaac acggtgaccg
     1861 cgagcccgcg cagcgccgcg acgatgcgcc cgcgaccgac cgggttcgcc gtgtgcacgt
     1921 acacctgggc cggcagcggc cccccctcga cctgcgccgc cgcgatccag cacgcgacgt
     1981 cgtagccggt cggcacgtcg gcgccgaggt catgatcgag gctcaccgcc tcgaccccgc
     2041 cggctgcgag caacgcgatg cactcggccg cgctgcgaac gatcacccac gcatcggggt
     2101 ccggcagccc cggcacctcc tccggcatcc gctcatcgtc gaggaacacg cgcacccccg
     2161 tgttctagca ccagctctcg cccggctcga cgatccgggc caacttgcgc gccaagatcc
     2221 gccgggcgca catccgacct ctcgcctcac tgcgcagcat ctgcgacgtg cccgtccacc
     2281 ggtgccacgt catacgaacc atgtcgcttc ggatcgccgc cgtcgtcgct tcgccgcacc
     2341 cgccccgtgc tctgctccgg ggcctcacga caggccgcgc cagcgatccc gctcagcgcg
     2401 gctcgcctgc gacgacctcg acgtcgacgc cgcgcgcgag cccaagcacc agcctcgtcc
     2461 cggccggcac ccggctcaag ctgtagaaca gccgcatctc gccgccggtg accggatgtc
     2521 cgtcgacacg gaccacctgg tcgccgacca cgatccccgc ctgcgccgcg ggacccccgg
     2581 cacgcaccgc cgtgatcgtc agctccttgc cggtcgtgag cccgaggtca cccgcctgct
     2641 cgccgtcttt gatccgcagg cgcgtcaact tcatcggcgc cagctcgacc accggacccg
     2701 ggcccgcgat ctcgagcccg aagcccgtaa actcgtaccc gttctcgacc atcgcgcgcg
     2761 gcatgatcat cacttgcacc gcgccggcgc tcaccctctc gagctcgaag cgcccctgca
     2821 cgtccgtcac ctcgccggcg gccatcggct cctgcgagga gtagacccgc tgctgcccct
     2881 tcggcgtcat ccgcacctgc atgcctggca ccccctcacc ggtctcgacg tcgatcaccc
     2941 gcccgcgcac cgtcacacgc gcgccgagta cttgccctcg ccgagcccgc cgagctcgaa
     3001 cctcccgtcc tgatccgtca gcctgcgccc cccgacatcc ccgaagaagg cgtccatgcg
     3061 cgccccttgc agcacacccg gcatcgccgg cccgtcgggc tcgcgcgccg gctcgacgaa
     3121 cgcgtcgagc aagggcccgc ccgcgctgtc gaccacgcgc ccggtgatcc gcccgcgccg
     3181 cgactcgacc acgagccgaa cctcgcgcac cgccccgacg acgacctcgg ccgcgacccc
     3241 ctccagatcc tccgtcgcgc cgggggcccg cagcgcccgg tggtcggcgc gcgccgtcac
     3301 ccgatacgcg cccgcaggca gatgcgcgaa ccgaaagctc ccgtcgtcga ccgtgctcgt
     3361 ccgcagccca tcgggcccgt ccccgcggac atgcacctcc acgcccttga ccggctcgcc
     3421 cgactcatcc ttgaccgtgc cgcgcagctt gccggtcgcc ggcaacgcga tgcgcacgcc
     3481 ctcgagatcc tgcccatcaa gcaacgcgac ctcgaccgcc gcggtcggat actcatcaga
     3541 atttgcgcgc agccggtagc gtccggccag cagccctgcg agctcgaagc tcccgtccgc
     3601 cagggtcacc gcgcgcacct cgctggtcgt gtgagcacgt ggcgcctgcg ggtcgatctg
     3661 cggctcggcg tgcagccaga tgtcacgcac acccgcgccg ccggccagca cctgcccgcg
     3721 gatcgcctgc ccgcgctgca cctcccagcg cagccccgag acggatgcat cgctaacgac
     3781 cacctgcggg tactccggca gcgccaccgc ccctgcgcac tcgagcttca cgccgtacgt
     3841 cccgggcaac acgccttgca catacacgct cccgtcgaga tcgatccccg aggcgcggct
     3901 ggaccggccc gcctccgtca gccgcagata cccgcccgcg cacggatctc cgcccgcgcg
     3961 cacgacggtc cccgcgacca cgtgggccgg gtgcgccgcg atctcgatca cctccgacgt
     4021 ctcgccgagc ccgagcaccg cgctctcggg cgccatgcca taggcctcgt cgtgcttcgc
     4081 ctcgagcttg taggtgcccg gaagcaggcc ctcgacgcgg aagttgcccg acgcgtccgt
     4141 gatcgccggc ccgaccgggt tccacgcgtc gctcgcctgc gcatacaccg atgcccccgc
     4201 cagcggcgcg cggtcacctg ctcgcaccac ccggccgacg agcaccgact ccggagtcaa
     4261 ccgaagttca aaccactgcc ccggcgccct tccaggcgcc gagccctgcg cgtagccggc
     4321 cgcccgcgcc gtcacgtaca cctggcccgg cgcgacccac agcaaaaatg tcccgtcggg
     4381 ccccgagcgc gcgtcggcgt cgcccgcctg cacctgggcc ccctcgagca cgccgccgct
     4441 gaggtcgtgc acgaccccgc gcagctcgac cccgccgcct tgcagcacga cgtcgatgtc
     4501 tcgcgcctcc tgcccggccc gcagcgcgac gagcgtgcgc gccctcgtcc cgcggcgatg
     4561 ttgccccggg atgaagtgtg gcgcgctggc gcggacgaag tacgcgaccg gccacagcgc
     4621 cgtgatgtga tagccgccgt cggcctcgct ctgcacgcag cgcacgtcct cggcgtcacg
     4681 gcgatcgagc gtccgcgaga acccggcgac gcacacgctc gccccggaga tccctcgccc
     4741 ctgttcgtcg cgcacgcgcc cggacaggct cgcctgctgc accacgcgcg gcacatccgg
     4801 cgtcccgacg cgctcgcccg tcgccatcga ttcgacgacc acggtcgcct ctgcggtctc
     4861 cggcgcatct gtccgcgagg acagccacca cagcagcgac ccgaggacgc cgatgagcac
     4921 gaaccatttt cctcgctcca tcggccctcg cagttcaacg atcgagcagc acccccagct
     4981 gatagcggcg cagcactggc gccttgttgg ggtccgtgaa gtacgaactg cgcgcgggat
     5041 cgcttattcc atggaaatcg agctcgccga gcaggtgcac gaccccctcg tggtcgaggc
     5101 gctgcatcgc gtcgagctcg tcgcgcgtca cgccgacaat ttgtaagaac agcacggagc
     5161 cgaagggcgt gtagatcttg cgcagcagcg ggtcgggcac gcaggcgatg catgtcaacg
     5221 cgctctcgat gcccggcgcg atcgatcccc cgaggtccgc gcagtgcccc gtatcgaagc
     5281 cctgacccgt cccgagcacg tagcgcccga gcccctgcag cgcccgcagc ggccacgtcg
     5341 gcggcaccgt ctcgtccgcc gcccgcggga cccgcatcgt cagctcgaag ccgaagcccg
     5401 agacatccgg gtcggccgag ctcttgtcga acagctcgct gagcccgtac gtgacgaagt
     5461 gccagctgtc gggcctgcgc gcctcgtaca ccgcgatcgc aggcagcggg ctctcggcgt
     5521 cgggctcgta ggcgcgctgg ctggcgaact ggtgcgggac ctggcctaca tacaaccgcg
     5581 ccagcgcgtc gtcgatcgcc gcccagccgg gcgcgtcctg cccgtctgct ccgtccgtca
     5641 tggcgctcac aggcggcgtt cgaactcggc catgaactcg cacagcgcgt cgacgccggc
     5701 ctcggggaag gcgttgtaga tcgacgcccg gaacccgccg acgaggcggt ggcccttgag
     5761 cccgtcgaag ccggccgcct gcgcctcctt gatgaagcgg ctggtcagcg cctcgcgctc
     5821 ggcctcgggg gcgccgttgg ccgtccatgt cacgttcatg cgcgagcgcg agcccggctg
     5881 cgcgtgcggc cggaacacgc tgctgcgctc gagcgcggcg tacagcttgt tcgccttgcg
     5941 ctcgttgacc gcgccgatct ccgtgaggcc gccggcgtcg cgcatccact cgagcaccag
     6001 cttgagcacc agcacgccga aggtgttcgg cgtgttgtag aggctcggct ccttggcgtg
     6061 ggtcgcgtag cgcaggatca ccggcacgtg cgcggccggc tttttattga gcaggtcctt
     6121 gcggatgtag accagcgtca cgcccgaggg cccgaggttc ttctgcgcgc cggcgtaccc
     6181 gaacgccgcg ttgccgagcg ccatcggtcg cgagccggcg tggctcgaga agtcgatcac
     6241 gagcggcacg ccgccctgcg cctcggggat ctccgggtat tccgtcccga agatcgtgtt
     6301 gttggtggtg atgtgcaggt agctcgcgcc cgcgtacgtg tccgccgccg ggaacgcggg
     6361 gatgcggtcg tagttggtcg cctccgagct ggcgaccacc gtggtctcgc cgagcccctg
     6421 gctctcgatg atcgcctttt gcgaccacac gccggtgtcg acgtacgccg ccggcttgcc
     6481 ggcctcgcgc aggttcatcg ccagcatcgc gaattgcagg ctcgcgcccc cctgcaggaa
     6541 cagcacctgg tgcgtctcgg ggatcccgag cacctcgtgg cacagcttct cggcgccgaa
     6601 ggtcaccgcc tcgtagggct tcgagcggtg cgagatctcg aggatcgaca tgccgacccc
     6661 cttcgcgtcc ggggcgtggc cgtcggcacg cagctcgcgg acggcctcgg ccgcgcgctc
     6721 gaacaccacc ggcggcaaga tcgccggccc ggccgagaag ttccacagct tcggtttttc
     6781 gctcgccatg gcctgggaag ctagcgcacc gccggccgcc gcgacagccc cgaaaatctc
     6841 ccagatttcc ccagcgtcag cgggccgtca cgcagccctt ttcgttgtcg gcgcagcgga
     6901 cccggacgtg gaagtggacc ttgtggccct ccacgtggcg aatgatctgg tgcttgggct
     6961 tgcgcaccgg ccactgcagc cagtgctcga gctgctcctt ggtcgcaccc tgctcgcgcg
     7021 ccaccttgta gatccgcttt tgcagcccaa aatcgaggaa gatgtactcg atctggccgc
     7081 ggtcgatgaa cgtgcgcatc agctgccagg tcgccttcca gtcgatgtcg ttggcggtga
     7141 tgtgacgctt accctccgcg gcggccgtca gcggcatgcg gatgtcgatg tcgcggccgc
     7201 tctggtgcga cttgtgcggc cgaaaccggc cgccgcgcgc ccggctgatc ccgccgatca
     7261 cgatcgatcg gccaaagccg gtctggtgcc ggaaggtcgc gatcgcggcc atcagctcgg
     7321 cgatcgcgtg ggacgagccg tagctctcgt cgggcttgcg gcggacgtac aggtcgggcc
     7381 gctccggcaa cgggacgccg cggaccaggc ggccgcggct cggcttgccg atgctgatcc
     7441 cgcccgaggg cacgcgcagc tgctgcagct tggcggccag gccggtgccg ctcagcggcg
     7501 ggggcagcgg gggcgcctcg cgccacacga ccagcttctt cttcgctacg accttcttgc
     7561 cggccttctt gttccaccgc ttgagcttct cgacctcgac gccgtactcc ttggcgacgc
     7621 tctcgaaggt ctcgccgcgc ttggcgacgt gctcgacgcg ctcgcgcggc agcggggcgt
     7681 cggccgtgac gatgcggagc gtcttgccgg ggagcggcag cttcggcttc ttcttgtcga
     7741 ggtggttcca gcggatgatg tcgtcgcggg acaccttgta gcgctccgcg atctcctcga
     7801 gcgtctcgcc ggggatgacc tcgtgcttga ccgaccccgg cttgcggtcg atcggccgcg
     7861 ggtcgctgac caccggcgga agctcgatct tcaccatctc gggggcgggc gggttggcca
     7921 gcagctcgcg ctcgatcgcg tcgagctcgc tctgcggcgt gctgtcgtcg gtgtccgcgt
     7981 cctcctccgc gtcgccctcg gcctcgtcct cgaggatctc gccgccgctg gtgacccccc
     8041 acgtgttgtc gacctcgcgc gacaccggga gcaccggcgc cggcttgctg gcgaccggac
     8101 gcttcctcga cttcttcttg ctcttcttct tcttgctggt cttcttcttg ctgctcgcgg
     8161 cggccgcctc gggcacctgg ctcagcgaga aagcgcccgt cagcagcagc gccaaccaca
     8221 gcccgagtac gccgaggagg cggtcggtgc ggccaggaag gcgcgtatgg ggtcgctgcg
     8281 cgggcatggg agccgagtcg gtcaccgtag cagacgccgg gggtcccgtg tacttgcggc
     8341 ccccgggccc tattcccgcg aggacgcggg gggcggccgt cacgggcctg cggcccgatc
     8401 gccgcgaggg tggcatcgac ggtcgtatct cggaaaattg ctgttgcaag acgcttgcag
     8461 gtggcctctc gccgcgcgaa gcaaacctcg ttcggcacgc ctctgcggcc ggaatttcgg
     8521 ccccgtggag atctgcacgc gccagcgcct gcggactgcg cggggcacgg gagggcgcgg
     8581 gcgcgcggcc ctcttctgcg ggcctgcgcg tcgcgacttc cttactactt ccttactact
     8641 tcactacttc ttcactactt cgccttcttc ttcttgctcg gcgcctcggg ctcggcgtcg
     8701 gcgaccccga gcacgcgcag cacctcgggg tagaccttgt cggcgaagta ctcgctgccg
     8761 gccatcgtga agtggatgcc gtcgtcctcg cgcaggtcct ggtccttgcg cttgccgacg
     8821 gtggccttgt cgagcagcac gcccttggcg tcggtgagga acggggtggt atcgaggtac
     8881 gtaccggcct cgccgagctc ggcgatcgcc tcgctctgga cctggcggat gatcgtcagc
     8941 ttctgctcga agctgttgat ctccgacttc ggcaagccga gccacagcac cttgcgctgg
     9001 tcgacaccga cctccttgag gaagtcggac acgcgggtct tgtaggcctc gttccagccg
     9061 tcggacttcc acgcgacgcg cttgcccttg ccggacttcg gcgtcaggtc ctggccgtcg
     9121 ttgccgccca tgatcacgac cacgaggtcc ggcgtcttcg cctcgacctg cttcttggcc
     9181 tccgactccc agtcatagaa gtcggggcgc gccaggccgg tcgcgctctt ggccttgcgc
     9241 tcgc
//
LOCUS       DASWNJ010001422         2113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_202477, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001422 DASWNJ010000000
VERSION     DASWNJ010001422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202477"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..1550
                     /locus_tag="VGB85_23670"
     CDS             228..1550
                     /locus_tag="VGB85_23670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HEY0137111.1"
                     /translation="MTTSVSQGSIGTLTNGDSTSVTGGEGTGTGGETVGSATGPGTSN
                     ISVSASEGDSGPKFDLGSTPDVGSGCGDGDGGMLDTSYIWIPATSGGWVSKINTRTMI
                     EEARYLTGPNGAGEDVSRTAVSGDGRFVVVNGRGSGRSTAVAANVEDCKDANGDGVIT
                     TSTGPGDVKPWGTEECIVWSLVHAAWSGSQMHGPRGITWTAGEWSEAQCAFINPKVWL
                     GYLSGDGSAHMVRLDGPTGVVEQDLIVPGWVGNGFAPYGGALDPLQRPWFTGLQGELA
                     RVEVSQNPITVTRFPQPGEIQSYGMTVDPDGNPWMGGCTGPVSTYDVNANTWITIPGT
                     GACHRGMAVDHDFHVWVASNQPCGLVEIDGKTRTLVAQHQLAQCSIPIGVSVDVDGFV
                     WLVDQAGWAWKIDPKNPGGMQMMMVEGSHYVYSDMTGGQLKSVSPPPA"
     gene            complement(1583..>2113)
                     /locus_tag="VGB85_23675"
     CDS             complement(1583..>2113)
                     /locus_tag="VGB85_23675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137112.1"
                     /translation="YTRTNLAELAAHCGTAEPAALTALNLVGVDSSTTPLDLGDARFL
                     RNLRTLGLDSLPGKDYAAVLSCPHLQSLEALALGSCYADTDAAFDALVSIEPPPNLHY
                     LDLTGWKPTSEQAATFAASPLGRQLWGLQMMSSYVQPDAWHAFYQAGLPLVGSGLFDA
                     GAPQEYTTSTTFREEV"
BASE COUNT          320 a          697 c          801 g          295 t
ORIGIN      
        1 cacgactgac aacggtcgtg gccggcaggc gccgctcgcc gggggatcca cgcgaggcgt
       61 cccgtgcccc cgggtgaagt cccgcgcggg gacttgcctg tcgctggtcg ccttgcgata
      121 agcgtggagt gctggcgaac cgcgttcgaa cccctctcgc tctgatctct ctcgtcgcgt
      181 ggagcgcctg tggcgactcg gggcgcgagg ccggcagcga cagcgctgtg acgacctcgg
      241 tctcgcaggg cagcatcggc acgctgacca acggagacag cacctcggtc acgggcggcg
      301 agggcaccgg caccggcggc gagacggtgg gcagcgcgac gggtccgggg acgagcaaca
      361 tctcggtgag cgcctcggag ggcgacagcg ggccgaagtt cgacctcggc agcacgcctg
      421 atgtcggcag cggctgcggt gacggcgatg gtggcatgct cgacaccagc tacatctgga
      481 tcccggcgac gagcggcggc tgggtctcga agatcaacac gcgcacgatg atcgaggagg
      541 cccgttacct caccggcccc aacggcgccg gcgaggacgt gtcgcgcacg gcggtctcgg
      601 gtgacggtcg cttcgtcgtc gtcaacggtc gtgggtccgg gcggtcgacc gcggtcgcgg
      661 ccaacgtcga ggactgcaag gacgccaacg gcgacggcgt gatcacgacc tcgaccggcc
      721 cgggcgacgt caagccgtgg ggcaccgagg agtgcatcgt gtggtcgctc gtgcacgccg
      781 cgtggtccgg cagccagatg catggcccgc gcggcatcac ctggaccgcc ggcgagtgga
      841 gcgaggccca gtgcgccttc atcaacccga aggtgtggct cggttatttg tccggggacg
      901 gctccgccca catggtgcgc ctcgacggcc cgaccggcgt ggtcgagcag gacctgatcg
      961 tgccgggctg ggtcggcaat ggcttcgcgc cctacggcgg cgcgctcgac cccctgcagc
     1021 gcccgtggtt caccggcctg caaggcgagc tggcgcgcgt cgaggtcagc cagaacccga
     1081 tcacggtgac gcgcttcccc cagcccggcg agatccagtc gtacggcatg acggtcgacc
     1141 ccgacggcaa cccgtggatg ggcggctgca ccggcccggt ctcgacctac gacgtcaacg
     1201 cgaacacgtg gatcacgatc cccggcaccg gcgcgtgtca ccgcggcatg gcggtcgacc
     1261 acgacttcca cgtgtgggtc gcgtcgaacc agccctgcgg gctcgtcgag atcgacggca
     1321 agacgcgcac cctcgtcgcc cagcaccagc tcgcgcagtg ctcgatcccg atcggcgtca
     1381 gcgtcgacgt cgacggcttc gtgtggctcg tcgaccaggc gggctgggcc tggaagatcg
     1441 acccgaagaa ccccggcggc atgcaaatga tgatggtcga gggatcgcac tatgtgtaca
     1501 gcgatatgac cggcggacag ctcaagagcg tctcgccgcc gcccgcgtga tcgctgggcc
     1561 ccgccctcgc gcgggccggg agctagacct cctcgcggaa ggtcgtgctc gtcgtgtact
     1621 cctggggcgc gcccgcgtcg aacaggccgg agccgacgag cggcaggccg gcctggtaga
     1681 aggcgtgcca ggcgtcgggc tgcacgtagc tggacatcat ctggaggccc cagagctggc
     1741 ggccgagcgg tgaagcggcg aacgtggcgg cctgctcgga ggtcggcttc cagccggtga
     1801 ggtcgaggta gtgcaggttg ggcggcggct cgatcgagac gagggcatcg aaggccgcgt
     1861 cggtgtcggc gtagcagctg ccgagcgcca gcgcttcgag cgactgcaag tgcgggcacg
     1921 acagcaccgc ggcgtagtcc ttgccgggga gcgagtcgag gcccagcgtg cggaggtttc
     1981 gcaggaagcg ggcgtcgccg aggtcgagcg gggtcgtcga cgagtcgacg ccgacgaggt
     2041 tgagggccgt cagcgcggcg ggctcggccg tgccgcagtg ggccgcgagc tcggcgaggt
     2101 tggtgcgggt gta
//
LOCUS       DASWNJ010001423         3429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_407811, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001423 DASWNJ010000000
VERSION     DASWNJ010001423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407811"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VGB85_23680"
     CDS             <1..550
                     /locus_tag="VGB85_23680"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY0137113.1"
                     /translation="DVPYRSNSYQFPLMCTWELEDNDQLPINCIDWYQAEAFCLWSKK
                     RLPTAIEWGWAARGREEQRRYPWGDEPPSCELAVVDQNKDDDDRGCGRNRPWPVGSKP
                     RDVTRDGVLDMLGNIGEKTSTGMTAEERSSRLTEGASWATAASIRFPIDVGSAAGRDA
                     YTDKSGVRCVKDVGLPPCAVPN"
     gene            1042..1785
                     /locus_tag="VGB85_23685"
     CDS             1042..1785
                     /locus_tag="VGB85_23685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137114.1"
                     /translation="MSNHNVISVALRALRCTCVAALVALPGCDDGVHPDAATADGSSL
                     SADERDYVADLAWRYLEQEVSDEEARQIDAEIAALSRAQMRLMNKELQLLGDADSNQA
                     RLGDGLTELAIERGVPMFALGQEDIDRVAAELGAGVSSRAFCETWSPSPAVSMSGFNS
                     ASAGTTASWTDRRSTNWYEYGGCDHRFYFSGKRTLIDGKSTAYDKWIASYPSILAQRT
                     YSSTIAVMGNYRAEAAGIYNVSASMVKAF"
     gene            complement(1839..3137)
                     /locus_tag="VGB85_23690"
     CDS             complement(1839..3137)
                     /locus_tag="VGB85_23690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137115.1"
                     /translation="MIRPPARPSMPSTSQLAIEHDGEHSLCLVVRGQRVVPGDDLSDR
                     FGFDIARLDERLLRILVTLAVRRHGGGPGWMTAEELGLLALNGASGSATAKLLEVSLR
                     GSTRVGPQLIEFRPAGATGGGRSRGPYRLGVPAESTVLDEAACWCFLTGRVLAEGMAD
                     GCSLADHLAQARAAMHAGRFIEAQALTQMALRSMFEGVQELRRATERDRCYWLAQTCL
                     LLSNVELEIGATRLGLLAVARAQRCFERIRHPEGSASALLVEANLRGQIDDTQERRRS
                     YVAARNALVRLDDAGRGPRRGMQRAIYIGTLGQRMSLLGQTRQAARRLRMAHQLCEAA
                     SSRTWAAIWEVRLGQNALTAGDRPAAERFMASALEAAEAMTPSGYASLVRGMSELSLA
                     TGRLDEAERWIVCARTVGEKLGMGHQVLLADRLMARLERL"
     gene            complement(3134..>3429)
                     /locus_tag="VGB85_23695"
     CDS             complement(3134..>3429)
                     /locus_tag="VGB85_23695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137116.1"
                     /translation="AAAEALATALVRGCRLRGVEDGDRPWIASAVALNNAGIYCLSEL
                     PNGRRTANICGDGDGAACSPTRYVMATIVARLRGDVEPAACRPVDMRAVECGP"
BASE COUNT          566 a         1169 c         1174 g          520 t
ORIGIN      
        1 cgacgtcccg taccggtcca attcctacca atttcctctg atgtgcacct gggagctcga
       61 ggacaacgat cagctcccga tcaactgcat cgactggtat caggcggagg cgttctgcct
      121 gtggtccaaa aagcgcctgc cgacggcgat cgagtggggc tgggccgccc gtggacgcga
      181 ggagcagagg cgttacccgt ggggcgacga gccgccctcg tgcgagctgg ccgtcgtcga
      241 tcagaacaaa gatgatgacg acaggggctg cggtcgtaac cgtccctggc ccgtcgggag
      301 caagccgcga gacgtgaccc gcgatggcgt gctcgacatg ctcggcaaca tcggcgagaa
      361 gacttccacc gggatgacgg ccgaggagcg ctcctcgcgg ctgacggagg gggcctcgtg
      421 ggcgacggcg gcgtcgatca ggtttccgat cgatgtgggc agcgccgcgg gccgggacgc
      481 ctacaccgac aagagcgggg tccgctgcgt caaggacgtg ggcctgccgc catgtgccgt
      541 gcccaattaa cgtggctcgc cgagcttccg gagaccggta cggtgcccgg tcttcatttg
      601 gctggagtcc tccggagctc gccgcgctga cagacggcgg gaggatgtgc gggcggaggt
      661 gatggcggct ggcgaacgcg ccgaagtaac cgtgtcgtgt gcaagcctgg cggccggacg
      721 agggcgcaca gtctggccag gaacttgtcg gcgttcatgt cggcgtggtc ggcacggttg
      781 cgccaaggga ccttgaggtg gacgcgcacg cggccatccg ggagggcttg gacggcgtca
      841 tgggcgaacg gtaggcgcag cgaggagtcg gcacacgcac ccgtcggatt aatcaataat
      901 aactggtggt taggcgccgc tctcgccacc gttggccggt cgactcggac ccgacatcct
      961 cgccgagtcc cgagtctgtg atcgactgca tcgatgacgg agttcggcgg cttcacaccg
     1021 attcgaaaaa ggatgtcaat catgtcgaac cacaatgtta tctccgttgc gctcagggcc
     1081 cttcgctgca cctgcgtcgc cgcgctcgtc gccctcccgg gctgcgacga tggcgtccac
     1141 cccgacgcag ccacggccga cggatcgtct ctctctgcag acgagcgtga ctatgtcgca
     1201 gatctcgcgt ggcggtacct cgagcaggaa gtcagcgacg aggaggcgcg gcagatcgac
     1261 gccgagatcg cggcgctttc gcgggcacag atgcggctca tgaacaagga gctgcagctc
     1321 cttggagatg ccgactcgaa ccaggcccgt ctcggcgacg ggctgaccga gctcgccatc
     1381 gagcgcgggg tcccgatgtt cgcgctcggt caagaagaca tcgaccgggt cgccgcggag
     1441 ctcggtgccg gcgtgtcctc gcgggctttc tgcgagacgt ggagcccgtc gcccgcggta
     1501 tcgatgtccg gcttcaacag cgcctccgcg ggcacgacgg cctcgtggac cgatcgtcgg
     1561 tcgacgaact ggtacgagta cggcggctgc gatcaccgct tctacttcag tgggaagcgc
     1621 acgttgatcg acggcaagag caccgcctac gacaagtgga tcgccagcta tccgtcgatc
     1681 ctcgcgcagc gcacctactc gagcacgatc gccgttatgg gcaactacag ggccgaggcc
     1741 gccgggatct acaacgtctc cgcctcgatg gtgaaggcct tctgacccgg acgccggtgc
     1801 gtccgtcacc agcatgacgg acgcgccggt tcgcacgttc atagtcgctc gagacgcgcc
     1861 atcagacggt cggcgagcag cacctggtgc cccatgccga gcttctcacc gacagttcgg
     1921 gcgcacacga tccaccgctc ggcctcgtcg agccgcccgg tcgccaggga cagttcgctc
     1981 atcccgcgca cgagcgaggc gtaccccgac ggcgtcatcg cctcggccgc ctcgagcgcg
     2041 ctcgccatga accgctcggc cgccggacga tcgcccgcag tgagcgcatt ttgtccgaga
     2101 cgaacctccc agatcgccgc ccacgtccgg gaggacgccg cctcacatag ctggtgggcc
     2161 atcctcagcc gccgcgccgc ctgccgagtc tgaccgagca gcgacatccg ttgcccgagt
     2221 gtcccgatgt agatcgctcg ttgcatgccg cgccgcggcc ctcgtccggc gtcgtcgaga
     2281 cggaccaggg cattgcgcgc cgcgacgtac gagcggcgcc tctcctgcgt gtcgtcgatt
     2341 tgaccccgaa gattcgcctc gaccagcagc gccgacgccg acccctccgg gtgacggatc
     2401 cgttcgaagc agcgctgcgc ccgcgccacg gcgagcagac cgaggcgcgt cgcaccgatc
     2461 tcgagctcga cgttcgacag cagcagacag gtctgcgcga gccagtagca gcgatcgcgt
     2521 tcggtggcac gacggagctc ctggacgccc tcgaacatcg atctcagggc catttgagtc
     2581 agcgcctgcg cctcgatgaa gcgtccagcg tgcatggcgg cccgggcttg ggcgaggtga
     2641 tccgcgaggg agcaaccgtc ggccataccc tcggcgagca cgcggccggt gaggaagcac
     2701 cagcacgccg cctcgtcgag cacggtcgac tcggccggaa cgccgagccg gtacggacca
     2761 cggctgcgtc cgccgcccgt cgcgcccgcg gggcgaaact cgatcagctg tgggccgacg
     2821 cgcgtcgagc cgcggaggga cacctcgagc agcttcgccg tcgcgctgcc gctcgctcca
     2881 ttgagtgcga gcaggccgag ctcctcggcg gtcatccacc cgggtccgcc cccgtggcgg
     2941 cggaccgcga gggtgacgag gatgcggagc aggcgctcgt cgagtcgggc gatgtcgaat
     3001 ccaaagcgat ccgacaggtc atcgccgggc accacacgct ggccacgcac gacgaggcac
     3061 agggagtgct ccccgtcgtg ttcgatcgct agctggctcg tcgatggcat cgagggccgc
     3121 gcgggcgggc ggatcacggg ccgcactcca cggcgcgcat atcgacgggc cgacacgctg
     3181 caggctcgac gtcgccgcgc aagcgggcga cgatcgtggc catgacgtac cgcgtcggtg
     3241 agcatgcggc gccatccccg tcgccgcaga tgttcgcggt gcgcctgccg ttcggtagct
     3301 ccgaaaggca ataaatgccg gcgttgttga gcgcgacggc ggaggcgatc catggccgat
     3361 caccgtcctc gaccccgcgc aggcgacaac cacgcaccag cgcggtcgcc agcgcctccg
     3421 cggcggcgc
//
LOCUS       DASWNJ010001424         4745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_236803, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001424 DASWNJ010000000
VERSION     DASWNJ010001424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236803"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="VGB85_23700"
     CDS             <1..1171
                     /locus_tag="VGB85_23700"
                     /inference="COORDINATES: protein motif:HMM:NF040941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibrinogen-like YCDxxxxGGGW domain-containing
                     protein"
                     /protein_id="HEY0137117.1"
                     /translation="GEPETSGTTHVPACGDGTVDPGEACDDGNQADDDACLGNCVAAR
                     CGDGVVESGVEACDDGNEDDSDACLPTCEAASCGDGVVQAGVEECDDANKLETDECRS
                     TCKHAKCGDGVVQAGVEACDDGNADDLDTCSTACEEQVPAASCKQILALAPAATSKVY
                     LIDPDGDGVQDPFKTFCDMETDGGGWTLILNRNVNSDNTGQPDINTTNGAFDNTRASN
                     WNFDIDRFWTGATQVVFADRQNDDCSNCKITDYHSAIRVEKPAGQVYKTDCLGASQMV
                     SAHKLVGPNAGTSGTAYQCGTSLGWGSCGGKVCHLGTHHKDTALDADWGQNLWQEMHF
                     PTTYSAAKSAGDFMVEPSAYCRSCGGGLPANFNESSTCCLSKQKNAKGRWTLWVR"
     gene            complement(1193..1852)
                     /locus_tag="VGB85_23705"
     CDS             complement(1193..1852)
                     /locus_tag="VGB85_23705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137118.1"
                     /translation="MALTPVSLLPLLLLVLAPACVINKDLLDGATSGGTSSGEDPGGS
                     TEPTTGGPEGEGSTSAPGTTTGDATTGDGATTGSTTTGDTDGPMQSELIIFGMDEPLC
                     MPVCAVDKDPATPALEASCNLFAVDVETQDEVAIAHCMQVGDEWVLPAGQVRCYEELI
                     DRGGETSSPIDQMSPVCVDGGFNVEFLVILSEPLPEGVVLGATCEASSDPEKDCPKLD
                     I"
     gene            2041..3705
                     /locus_tag="VGB85_23710"
     CDS             2041..3705
                     /locus_tag="VGB85_23710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137119.1"
                     /translation="MTTPTSRTPTKAVFYFRTLDRWPGGNADMLTIKVTLRLTPTPAL
                     EAQFYSCFDGNHAWYPPRSVTIDAADALAGLLITTSSLAARGSQSLTITMLDRNEDEW
                     DSEWAEIEVLLFAGDDGPETSEAAQLRLLRTTSKTKGTEPDPELEQAFELAQQVTGVG
                     RVFQFANTQDALSAAYRAQGQDGVQDLDYKRDATPRYLPPETDPVFAKLRGGASKKQP
                     AAAPVAKAPARETLLVYRLDEAPALLTRELPPDASTRKCLFYSARRDSPLTSAMYKAI
                     PEPSHAGTTDALKVAARISQIASQSPDFARKLRPDEVPAGGSLIGGGYASGFHGGDAE
                     AVERWLDEQGVTRISLRTFLDQLIVLRLVDEDDAPIDQVDKYAGGPPISTVDALDHDG
                     LFVFERDRDHAEFKLAAVTDEGLDDEDDGWVVLHANDLSPDTVHTVVIPAAPASKPPA
                     AKKTAAKKTATKKTAAKKTASKKTAAKKVAKKTAKKTAAKKTAAKKVAKKAATKKTAT
                     RKTATKKAAAKKAAAKKTTKKTAAKKAATKKVAKKAAAKKTAARKR"
     gene            complement(3746..4396)
                     /locus_tag="VGB85_23715"
     CDS             complement(3746..4396)
                     /locus_tag="VGB85_23715"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0137120.1"
                     /translation="MSDEETGLVQISGRSSSHFTRLVLLFAHELGVPFELRPIYDMTV
                     VDPETYAGNPALKMPTLRRGGSLVFGAENICRTLAELSSARPRIVWPEELRTDVARNA
                     QELVWHGMAAQVQIIFGTVVAKLPADNIYFEKGRQGLEGALRWLDDNLARVLGSLPEP
                     RDLSLLEASLFCLVDYIAVRGTASLDPFPALVRFAESFAQRPSAQRTRYHFDPPKA"
     gene            complement(4393..>4745)
                     /locus_tag="VGB85_23720"
     CDS             complement(4393..>4745)
                     /locus_tag="VGB85_23720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase"
                     /protein_id="HEY0137121.1"
                     /translation="DPGAIQIAPDELRTVRARLEREDLTVLAYRFAGDRFCTAQRFAA
                     YAEALGERFVATVLPSAAANPDPPPFFKDVVGCPHSVVTAHLIDAAGEPTAAARDEIL
                     AFFIRRLASGKETQ"
BASE COUNT          843 a         1699 c         1574 g          629 t
ORIGIN      
        1 cggcgagccg gagaccagcg gcaccaccca cgtcccggcc tgcggcgacg gcacggtcga
       61 ccccggcgag gcgtgcgacg acggcaacca ggccgacgac gacgcgtgcc tcgggaactg
      121 cgtcgcggcc cgctgcggcg acggcgtggt cgagtccggc gtcgaggcct gcgacgacgg
      181 caacgaggac gacagcgacg cgtgcctgcc gacctgcgag gcggcgagct gcggcgatgg
      241 tgtcgtgcag gccggcgtcg aggagtgcga cgacgccaac aagctcgaga ccgacgagtg
      301 ccggtcgacc tgcaagcacg cgaagtgcgg tgacggcgtc gtgcaggccg gcgtcgaggc
      361 ctgcgacgac ggcaacgccg acgacctcga cacctgctcg acggcgtgcg aggagcaggt
      421 ccctgccgcg agctgcaagc agatcctcgc gctcgcgccc gcggcgacga gcaaggtcta
      481 cctgatcgac cccgatggtg acggcgtcca ggacccgttc aagaccttct gcgacatgga
      541 gaccgacggc ggcggctgga ccctgatcct caaccgcaac gtcaactccg acaataccgg
      601 ccagccggac atcaacacga ccaacggcgc cttcgacaac acccgcgcga gcaactggaa
      661 cttcgacatc gatcgcttct ggaccggtgc gacccaggtc gtcttcgccg accggcagaa
      721 cgacgattgc agcaattgca agatcaccga ctatcactcg gcgatccgcg tcgagaagcc
      781 ggccgggcag gtttataaga cggactgcct cggcgcgagt caaatggtga gtgcccacaa
      841 gctggtcggc ccgaacgccg ggacctcggg caccgcgtac cagtgcggca cgagcctcgg
      901 ctggggtagc tgcggcggca aggtgtgcca cctcggcacc caccacaagg acaccgcgct
      961 cgacgccgac tgggggcaga acctgtggca ggagatgcac ttcccgacga cctactcggc
     1021 ggcgaagagc gccggcgact tcatggtcga gccctcggcc tactgccgca gctgcggcgg
     1081 cgggctgccg gccaacttca acgagtcatc gacctgctgc ctgtcgaagc aaaagaacgc
     1141 gaaggggcgc tggaccctgt gggtccgctg acgcccgcac cgcgcgagcg cgtcagatgt
     1201 ccagcttcgg gcaatctttc tccggatcgc tcgaggcctc acaggtcgcc ccgaggacca
     1261 cgccctcggg cagcggctcg ctcaggatca ccaggaactc gacgttgaag ccgccgtcga
     1321 cgcaaactgg gctcatctgg tcgatcggcg acgaggtctc gccgcccctg tcgatcagct
     1381 cctcgtagca acgcacctga ccggcgggga ggacccattc gtcgccgacc tgcatgcaat
     1441 gcgcgatcgc gacctcgtcc tgggtctcga catcgaccgc gaacaggttg cagctcgcct
     1501 cgagcgcggg ggtcgcggga tccttgtcga cggcgcacac cggcatgcac agcggctcgt
     1561 ccatgccaaa gatgatcagc tcggattgca tcggaccatc ggtatcgccg gtggtcgtgg
     1621 agccggtggt cgccccgtcc cctgtcgtcg cgtcgccggt cgtcgtgccg ggcgcgctgg
     1681 tcgacccctc gccctcgggg ccgccggtgg tcggctccgt cgagccgccc gggtcttccc
     1741 ccgagctggt gcccccggac gtcgcgccgt cgagcaggtc cttgttgatg acgcaggccg
     1801 gcgcgaggac cagcaggagc agcgggagca gcgagacagg tgtgagggcc atgcccctcc
     1861 ttctccgccc cccggcgatt ctgttcgccg cagtcgttta atttttcaca gcgacgcgtc
     1921 gggcccctgg gaggttgcct cggccggcgg ctcgtggtct cttcgcccgg cacctcgatg
     1981 cccgcgagcg cccggactcg gcgtcgcccc cccgaccctc accccccgat cgatcgaccg
     2041 atgacgacac ccacctcgcg caccccgacc aaggctgtct tctattttcg caccctcgac
     2101 cgctggcctg gcggcaacgc cgatatgctc accatcaagg tgaccctgcg gctgaccccg
     2161 acgcctgcgc tcgaggccca gttctacagc tgcttcgatg gcaatcacgc ctggtacccg
     2221 ccccgcagcg tcacgatcga cgctgccgac gcgctcgccg ggctgctcat cacgaccagc
     2281 tcgctcgccg cgcgcggcag ccagtccctc accatcacga tgctcgaccg caacgaggac
     2341 gaatgggaca gtgaatgggc cgagatcgag gtcctcctgt tcgccggcga cgacggcccg
     2401 gagacctccg aggccgcgca gctccggctg ctgcgcacga cctcgaagac gaaggggacc
     2461 gagcccgacc ccgagctcga gcaggccttc gagctcgcgc agcaagtcac cggcgtcggc
     2521 cgcgtgttcc agttcgccaa cacgcaggac gccctgtccg cggcgtatcg ggcccagggc
     2581 caggacggcg tgcaggacct cgactacaaa cgtgacgcga cgccgaggta cctgccgccc
     2641 gagaccgacc ccgtgttcgc caagctgcgc ggcggtgcga gcaagaagca gccagccgct
     2701 gcgccggtgg cgaaggcacc ggcgcgggag accttgctcg tgtaccgcct cgacgaggcg
     2761 cccgcgctcc tgacccgcga gctcccgccc gacgcgagca cgcgcaagtg cctgttctat
     2821 tccgcgcgcc gcgactctcc cctcaccagc gcgatgtaca aggcgatccc cgagccctcg
     2881 cacgccggga ccaccgacgc gttgaaggtc gccgcccgga tctcgcagat cgcctcgcag
     2941 tctccggact tcgcgcgcaa gctgcgaccc gacgaggtgc cggccggggg ttcactgatc
     3001 gggggcggat acgcgtctgg cttccacggc ggcgacgccg aggcggtcga gcgctggctc
     3061 gacgagcagg gcgtcacacg catatcgctg cgcaccttcc tcgatcagct gatcgtgctg
     3121 cgcctggtcg acgaggacga cgcgccgatc gatcaggtcg acaaatacgc cggcggcccg
     3181 ccgatctcca ccgtggacgc gctcgaccac gacggcctgt tcgtcttcga acgcgaccgc
     3241 gaccacgccg aattcaagct cgccgccgtc accgatgaag ggctcgacga cgaggacgac
     3301 ggctgggtcg tgctgcacgc gaacgacctc tcgccggaca ccgtccacac ggtcgtcatc
     3361 cccgcggcgc ccgcttcgaa gccgcccgca gcgaagaaga ctgccgctaa aaagaccgcg
     3421 accaagaaga ccgccgccaa gaagactgcg agcaagaaga ctgccgccaa gaaggtcgcc
     3481 aagaagaccg ccaagaagac cgccgccaag aagactgcgg ccaagaaggt cgccaaaaaa
     3541 gccgcgacca agaagaccgc gaccaggaag accgcgacca agaaggccgc cgccaagaag
     3601 gccgccgcca agaagaccac caagaagacc gccgccaaga aggccgccac caagaaggtc
     3661 gccaagaagg ccgccgccaa gaagaccgcc gccaggaagc ggtaggcctc gctatgacga
     3721 cccggcgatc cccggatcgc cgggctcacg ccttcggtgg atcgaagtgg tagcgcgtgc
     3781 gctgggccga cgggcgctgg gcgaacgact ccgcgaagcg cacgagcgcg gggaacgggt
     3841 cgagcgacgc ggtgccgcgc acggcgatgt aatcgacgag gcagaacagc gaggcctcga
     3901 gcaggctcag gtcgcgcggt tcaggcaggg agccgagcac gcgcgccagg ttgtcgtcga
     3961 gccagcgcag cgcgccctcc aagccctggc ggcccttttc aaaatagatg ttgtcggccg
     4021 gcaacttggc gacgaccgtg ccgaagatga tctgcacctg cgcggccatg ccgtgccaca
     4081 ccagctcctg ggcgttgcgg gcgacgtccg tgcgcagctc ctccggccag acgatgcgag
     4141 gccgcgcgct ggacagctcc gcgagcgtgc gacagatgtt ctccgcgccg aagacgagcg
     4201 agccgcccct gcgcagggtc ggcatcttca gggccgggtt tcccgcgtag gtctcggggt
     4261 cgacgaccgt catgtcgtag atcgggcgca gctcgaaggg cacgccgagc tcgtgggcaa
     4321 agaggaggac cagccgcgtg aagtgcgagc tgctccgacc gctgatctgc accaaccccg
     4381 tttcttcgtc gctcattgtg tctccttacc agatgccagt cggcggataa agaacgccag
     4441 gatctcgtcg cgcgccgcgg ccgtcggctc accggcggcg tcgatcaggt gcgccgtcac
     4501 cacgctgtgc ggacagccga ccacatcttt gaagaagggc ggaggatcgg ggttggccgc
     4561 cgcggacggc agcacggtcg ccacgaagcg ctccccgagc gcctccgcgt aggcggcgaa
     4621 gcgctgggcg gtacaaaagc gatcgccggc gaagcgatag gcgagcaccg tcaggtcctc
     4681 tcgctcgagc cgcgcccgca cggtccggag ctcgtcgggc gcgatctgga tcgcgccggg
     4741 gtcgt
//
LOCUS       DASWNJ010001425         4911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_61584, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001425 DASWNJ010000000
VERSION     DASWNJ010001425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61584"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VGB85_23725"
     CDS             <1..547
                     /locus_tag="VGB85_23725"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY0137122.1"
                     /translation="AANSGVGASMVALTRRLGLRIVSVVRREDAVAELRQQGSDLVLV
                     DGPDLAERARAAIAGGRLRLGIDAIGGEATARIAASLDDGGVAVNYGLLSGQNPQISG
                     RELVFRGVRLEGFWLVQWFGRHPPAEIARVYGELAGMLAEGTISVPIVARYPLSRVRE
                     ALEHAARDARGGKILLVPDSA"
     gene            566..886
                     /locus_tag="VGB85_23730"
     CDS             566..886
                     /locus_tag="VGB85_23730"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY0137123.1"
                     /translation="MDTDDRRKFERVSIAIAVRYSDHEEQVAVMSRDISLGGLFLGTV
                     TPAPVATTLDLRLHLPAPHGEVLARGKVMHCLQGIGMGVEFAGFDGDGESRLRHYLEL
                     LSAP"
     gene            888..1604
                     /gene="rpe"
                     /locus_tag="VGB85_23735"
     CDS             888..1604
                     /gene="rpe"
                     /locus_tag="VGB85_23735"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY0137124.1"
                     /translation="MTSPIHIAPSLLSADFARLGEEVLALDRAGADWIHVDVMDGRFV
                     PNLTIGPLVVEALRPHTARVLDCHLMIEEPERYVADFARAGADIITVHVEACRHLHRN
                     LQQIRGLPHHAGKRVLAGVSLNPHTPIESILHVLELCDLVLVMSVNPGFGGQGFIPQV
                     RPKIRALRAEIAARGLATDVQVDGGVTVDNIHEVAGDGANAFVSGSGVLRSKQFGRDY
                     AAAISAMRARAEAAYTGRPA"
     gene            1666..2757
                     /locus_tag="VGB85_23740"
     CDS             1666..2757
                     /locus_tag="VGB85_23740"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HEY0137125.1"
                     /translation="MFDRAKLPLRSGSALAVTLAAVALTWTTPARAQNPAPAPVPAPS
                     PANEPKVEAPKGTVGAQAAASSGATNLDDGKVATAIIPTQDVTHATELDLSAGGLFNS
                     GNARSLSMTAGGRFRIRRKAHEFSASLAGNYGRASVPGDDGASISRSTAGNVQARLRY
                     DYFFHPRVSFFAMGTTRYDPFLGLTSRIRVDPGFAFFAIQQPKHRLWGELGYDFQYDV
                     RRVYDGVRQDCQRGGVTVQCHVLAVPNVPLSTSALVVDRTLAVHSMRMFAGYINQLGE
                     IVTFTTGLEYIQALSPLRSSDTDPPKPGDPVSPRVRMWVNWDAALTVAVRKNLAFATT
                     FTLRHDNAPLPGVRRLDTITAVTLTYRFF"
     gene            complement(2795..3874)
                     /locus_tag="VGB85_23745"
     CDS             complement(2795..3874)
                     /locus_tag="VGB85_23745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137126.1"
                     /translation="MRAAITLLSLGTGLLACTPAPAIEDYPAELEEAYCQWQHGCHQY
                     ESVRDCIDDNAIERDPGYMYLLAAQAAGHVEYDREQAARCLDAMQGRSCEYEEEAFSA
                     CDNVFRGRIGRNGPCMSTAECAGNAVCGFDPTCTDMCCVGACRVFADPLAVGEPCSSS
                     AGCVPEAYCAFDPSTGAPTVCTALVEAGGDCSFGQGCVEEAICDGERCRKFELRGPGE
                     DCLGDFVDCAPPGECLSSAEGRWRCVAPPQLGAPCDDRISCARFDTFCDPNSQLCTLL
                     PGPGQGCGASDCVPYAQCQSEVEGAVDSEIAYTCVAKAGEGEPCGSRDDQYVECRPPF
                     QCVDGETCTLPEVESGPACPVPDAG"
     gene            complement(3877..>4911)
                     /locus_tag="VGB85_23750"
     CDS             complement(3877..>4911)
                     /locus_tag="VGB85_23750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137127.1"
                     /translation="RQYGVEPRCRAVNHMEVRLSMQQLAGVGALEAVPIEYMKEAVEV
                     GRIGYDKEAAATCLAYVRTRTCEYAWLHVWTEEELAGQAACDAVFKGRMGRNGPCVSA
                     SECAEESVCGFDPSCADMCCVGACRVLAQPLELGEPCGGNTPCAVDLFCDSDPNTGMS
                     TVCKASPTLGQPCPNGVCAGEAYCDYSGDALTCRARKAEGELCNGGNECEQPGVCQYN
                     DVDWTGRCYRPRDEGEACDPDGYDAQCIRVDNTCHLVDRVCVPLPGKDDYCPRNECAG
                     DFFCSQQQGGRCLPVADAGERCGYDQITYEETPCSGDHYCDTSVEQSTCKAPSGEAGC
                     PVPANPGQGT"
BASE COUNT          678 a         2012 c         1533 g          688 t
ORIGIN      
        1 cgccgccaac tcgggcgtcg gcgccagcat ggtcgcgctg acccgccgcc tcggcctgcg
       61 gatcgtctcc gtggtgcgcc gcgaggacgc cgtcgccgag ctgcgccagc agggcagcga
      121 cctcgtgctc gtcgacggcc ccgacctcgc cgagcgcgcg cgcgctgcga tcgccggcgg
      181 gcgcctgcgc ctcggcatcg acgcgatcgg cggcgaggcg accgcgcgga tcgccgccag
      241 cctcgacgac ggcggcgtcg ccgtcaacta cggcctgctc tccggccaga acccgcagat
      301 cagcgggcgc gagctcgtgt tccgcggcgt ccgcctcgag ggcttctggc tggtccagtg
      361 gttcggccgc cacccgcccg cggagatcgc acgcgtctac ggcgagctcg ccggcatgct
      421 cgccgagggc acgatctcgg tgccgatcgt cgctcgttac ccgctctcgc gcgtccgcga
      481 ggcgctcgag cacgccgccc gcgacgcccg cggcggcaag atcctgctcg tccccgactc
      541 cgcctgatcg ccgaaggagc tgcccgtgga caccgacgac cgccgcaagt tcgaacgcgt
      601 ctccatcgcc atcgccgtcc gttattcgga ccacgaggag caggtcgccg tcatgagccg
      661 cgacatcagc ctcggcggtc tgttcctcgg caccgtcacg cccgcgccgg tcgccaccac
      721 cctcgacctt cgcctgcacc tgcccgcgcc gcacggcgag gtgctcgccc gcggcaaggt
      781 catgcactgc ctgcagggca tcggcatggg ggtcgagttc gccggcttcg acggcgacgg
      841 cgagtcacgg ctgcggcact atctcgagct cctgagcgcc ccctgacatg acctcaccga
      901 tccacatcgc cccctccctg ctcagcgccg acttcgcccg cctcggtgag gaggtcctgg
      961 cgctcgaccg cgccggcgcc gactggatcc acgtcgacgt gatggacggc cgcttcgtgc
     1021 cgaacctgac gatcggcccg ctcgtcgtcg aggccctgcg cccgcacacc gcccgcgtgc
     1081 tcgactgcca cctgatgatc gaggagcccg agcgctacgt cgccgacttc gcgcgcgccg
     1141 gcgccgacat catcaccgtg cacgtcgagg cctgccgcca cctgcaccgc aacctccagc
     1201 agatccgcgg cctgccgcac cacgccggca agcgcgtgct cgccggcgtc agcctcaatc
     1261 cccacacccc gatcgagtcg atcctccacg tgctcgagct gtgtgacctc gtcctcgtga
     1321 tgagcgtcaa cccgggcttc ggcggccagg gcttcatccc gcaggtgcgc ccgaagatcc
     1381 gcgccctgcg ggccgagatc gccgcccgcg ggctcgccac cgacgtccag gtcgacggcg
     1441 gcgtcacggt cgacaacatc cacgaggtcg caggagacgg cgccaacgcc ttcgtcagcg
     1501 gcagcggcgt cctgcgcagc aagcagttcg gtcgcgacta cgccgcggcg atctccgcga
     1561 tgcgcgcccg ggccgaggcc gcctacaccg ggcgccccgc ctgaccgccc gctgaaattg
     1621 ccgcgctgct tggtttcgcg gccgtttggt ggcaaaaccg ccgccatgtt cgatcgcgcg
     1681 aagctccccc tccgtagtgg gtccgccctc gcagtgaccc tcgccgccgt cgccctgacc
     1741 tggacgaccc ccgcgcgggc ccagaacccc gccccggcgc ccgtgcccgc cccctcgccc
     1801 gcgaacgaac ccaaggtcga ggcccccaag ggcaccgtcg gggcccaggc cgccgcctcc
     1861 tccggcgcga ccaacctcga cgacggcaag gtcgccaccg ccatcatccc cacccaggat
     1921 gtcacccacg cgaccgagct cgacctctcc gccggcggcc tgttcaactc cggcaacgcg
     1981 cgctcgctgt cgatgaccgc cggcggccgg ttccgcatcc gccgcaaggc ccacgagttc
     2041 tcggccagcc tcgccggcaa ctacggccgc gcctcggtgc ccggggacga cggcgcgagc
     2101 atcagccgct cgaccgccgg caacgtgcag gcccgcctgc gctacgacta cttcttccac
     2161 ccgcgggtct ccttcttcgc gatggggacg acccgctacg accccttcct cggcctgacg
     2221 tcccggatcc gcgtcgaccc cggcttcgcc ttcttcgcga tccagcagcc caagcaccgc
     2281 ctgtggggcg agctcggcta cgacttccag tacgacgtgc gccgcgtcta cgacggcgtg
     2341 cgccaggact gccagcgcgg cggcgtcacc gtgcagtgtc acgtgctcgc cgtgcccaac
     2401 gtcccgctgt cgacctccgc gctggtcgtc gaccgcaccc tcgccgtgca ctcgatgcgc
     2461 atgttcgccg gctacatcaa ccagctcggc gagatcgtca ccttcaccac cggcctcgag
     2521 tacatccagg ccctgtcacc gctgcgcagc agcgacaccg acccgcccaa gcccggcgac
     2581 cccgtctcgc cgcgcgtgcg catgtgggtc aactgggacg ccgccctcac cgtcgccgtg
     2641 cgcaagaacc tcgcgttcgc caccaccttc accctgcgcc acgacaacgc cccgctgccc
     2701 ggcgtgcgcc gcctcgacac gatcaccgcg gtcaccctga cctaccgctt cttctaaaaa
     2761 gacgcgcctg tcccgaagga caggcgcgcg cgcctcagcc cgcgtccggc accggacacg
     2821 ccggccccga ctcgacctcc ggcagcgtgc aggtctcccc gtcgacgcac tggaacggcg
     2881 gccggcactc gacgtactgg tcgtcgcggg agccgcacgg ctcgccctcg cccgccttcg
     2941 ccacgcaggt gtacgcgatc tcgctgtcga ccgcgccctc gacctcggac tggcactgcg
     3001 cgtacgggac acaatcgctg gcaccgcagc cctgacccgg gccgggcagc agcgtgcaca
     3061 gctgcgagtt cgggtcgcag aaggtgtcga agcgggcgca cgagatccgg tcgtcgcacg
     3121 gcgccccgag ctggggcgga gcgacgcacc gccaccggcc ctccgccgaa ctgaggcact
     3181 ccccgggcgg cgcgcagtcg acgaagtccc ccaggcagtc ctcccccggc ccgcgcagct
     3241 cgaacttccg gcaccgctcg ccgtcgcata tcgcctcctc gacacagccc tgaccgaacg
     3301 agcagtcccc cccggcctcg accagcgccg tgcacaccgt cggcgcgccc gtcgacgggt
     3361 cgaacgcgca atacgcctcc ggcacgcatc cggcggagga gctgcacggc tcgccgaccg
     3421 cgagcgggtc ggcgaacacc cggcacgcgc cgacgcagca catgtccgtg cacgtcggat
     3481 cgaagccaca caccgcattg cccgcgcact cggccgtgct catgcacggc ccgttgcggc
     3541 cgatccgccc gcgaaacaca ttgtcacagg ccgagaacgc ctcctcctcg tactcgcagc
     3601 tgcgcccctg catcgcgtcg aggcagcgcg ccgcctgctc gcggtcgtac tcgacgtggc
     3661 cggccgcctg cgccgccagc aggtacatgt accccgggtc gcgctcgatc gcgttgtcgt
     3721 cgatgcagtc gcgcacgctc tcgtactgat gacagccgtg ctgccactgg caatacgcct
     3781 cctcgagctc ggccggataa tcctcgatcg ccggcgccgg cgtgcacgcc agcagacctg
     3841 tcccgagaga cagcagtgtg atcgccgcgc gcatcatcat gtgccctgcc ccgggttcgc
     3901 cggcaccggg cagccggcct cgccgctggg ggccttgcac gtgctctgct cgacgctggt
     3961 atcgcagtag tgatcgcccg agcacggcgt ctcctcgtag gtgatctggt cgtagccgca
     4021 ccgctccccg gcgtcggcga ccggcaagca gcgcccgccc tgctgctgcg agcagaagaa
     4081 gtcgcccgcg cactcgttgc gcgggcagta gtcgtccttg cccggcagcg gcacgcacac
     4141 ccggtcgacc aggtggcagg tgttgtcgac gcggatgcac tgcgcgtcgt agccgtccgg
     4201 gtcgcacgcc tcgccctcgt cgcgcggccg gtagcagcgc ccggtccagt cgacgtcgtt
     4261 gtactgacac acccccggct gctcgcactc gttgccgccg ttgcacagct cgccctcggc
     4321 cttgcgcgcg cgacacgtca gggcgtcgcc gctgtaatcg cagtaggcct cgccggcgca
     4381 gaccccgttc gggcacggct ggccgagcgt cggcgacgcc ttgcacaccg tcgacatccc
     4441 ggtgttcggg tcggagtcgc agaacaggtc cacggcgcac ggcgtgttgc cgccacacgg
     4501 ctcgccgagc tcgagcggct gcgcgagcac gcggcaggcc ccgacgcagc acatgtctgc
     4561 gcagctcggg tcgaagccgc agaccgactc ctccgcgcac tcggacgccg acacgcaggg
     4621 cccgttacgg cccatgcggc ccttgaacac cgcgtcgcac gcggcctggc ctgccagctc
     4681 ctcctcggtc cacacgtgca gccacgcata ctcgcaggtg cgcgtgcgga cgtacgcgag
     4741 gcaggtcgcg gccgcctcct tgtcgtaccc gatcctgccg acctcgaccg cctccttcat
     4801 gtactcgatc ggcaccgcct ccagcgcccc gacgccggcc agctgctgca tcgacagccg
     4861 cacctccatg tggttgaccg cccggcagcg cggctcgacg ccgtactgcc g
//
LOCUS       DASWNJ010001426         1913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_26170, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001426 DASWNJ010000000
VERSION     DASWNJ010001426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26170"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VGB85_23755"
     CDS             <1..593
                     /locus_tag="VGB85_23755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137128.1"
                     /translation="VNTDACSKQCVLTPKSLILGAGQPTPQYGSPNLGTAYNDNCPAG
                     QVLIGFTGSLKAGAHAAIKGVCAVPMLAVQDDAFVIKTGPGAALPQRGGLGDTPWMRV
                     CPADHVLVGFSGSAGTGVNQLTFSCAPLVVSEGVGAFTIGVGPATQLMVVGAVGGMPF
                     MPTSCPIGQVAGAQRLRAGTVVNAFGLGCSAVSLGF"
     gene            590..1147
                     /locus_tag="VGB85_23760"
     CDS             590..1147
                     /locus_tag="VGB85_23760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137129.1"
                     /translation="MTLARAPLAFTAGSLYLAAEDPTNPGREVAGGNIDGKPGNEVVT
                     CTVANKVRALDSAGKQLWISEMPLTCVSPTTADIDNDGIAEVIVTGGILDGATGALEV
                     KIATGSDVLAADLNVDGELDVVQPLGAFTAGGGVMAQTETEGRFAAIADLDLDGKPEV
                     AVIANAGRSRRSSVSSSAAATRRCA"
     gene            1197..1847
                     /locus_tag="VGB85_23765"
     CDS             1197..1847
                     /locus_tag="VGB85_23765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137130.1"
                     /translation="MLIWCYNPMAPTGVDIVRAPIDINGALDPALCLMGSAGNVNGGG
                     TPDVAVAGGVGYAVFDGKKLLDPNVAGPDTFLWIKQTLDCSSAATGSSVFDFGGDGSA
                     EVPYGDEQRLQIYKGSDGAVLFETCNTSGTLREFPLVADVDSDGHAHIVAVSNAYSSI
                     NCDGTKQRGIRVFGDSKGNWVRTRRVWNQHAYHITNVNEDGSIPAAAGQLDDARAQ"
BASE COUNT          305 a          685 c          671 g          252 t
ORIGIN      
        1 aggtcaacac cgacgcgtgc agtaaacagt gcgtgctgac gccgaagtcg ctgatcctcg
       61 gcgccggcca gccgaccccg cagtacggca gccccaacct cggcacggcc tacaacgaca
      121 actgtcccgc gggacaggtg ctgatcggct tcacgggcag cctcaaggcc ggcgcgcacg
      181 cggcgatcaa gggcgtgtgc gcggtaccga tgctcgccgt gcaggacgac gccttcgtga
      241 tcaagaccgg cccgggcgcc gcgctgccgc agcgcggcgg gctcggggac acgccgtgga
      301 tgcgcgtctg cccggcggac cacgtgctgg tcggcttctc cggcagcgcc gggaccggcg
      361 tcaaccagct gaccttcagc tgcgcgccgc tggtggtctc ggagggggtc ggcgccttca
      421 cgatcggcgt cggcccggcg acgcagctga tggtcgtcgg cgccgtcggt ggcatgccct
      481 tcatgccgac gagctgtccg atcggacagg tcgccggcgc ccagcggctg cgcgcgggca
      541 cggtcgtcaa cgcgttcggc ctcggctgca gcgccgtcag cctcggcttt tgacgctcgc
      601 tcgcgcgccg cttgctttca cggcaggcag cctctacctc gcggccgagg acccgaccaa
      661 ccccgggcgc gaggtcgcgg gcggcaacat cgacggcaag cccggcaacg aggtcgtcac
      721 ctgcacggtc gccaacaagg tgcgcgcgct cgactcggcg ggcaagcagc tgtggatctc
      781 cgagatgccg ctgacctgcg tgtcgccgac aaccgccgac atcgacaacg acggcatcgc
      841 cgaggtgatc gtcaccggag ggatcctcga cggcgcgacc ggggcgctcg aggtcaagat
      901 cgccaccggc agcgacgtgc tcgcggcgga cctcaacgtc gacggcgagc tcgacgtcgt
      961 ccagccgctc ggggcgttca cggccggcgg cggggtcatg gcccagaccg agaccgaggg
     1021 ccgcttcgcg gcgatcgccg acctcgacct cgacggcaag ccggaggtcg ccgtcatcgc
     1081 caacgccgga cggtcgaggc gctcgagcgt gagctcgagc gcagccgcga cgcggcgctg
     1141 cgcctgacgt acctgcggcc ggcggccgcg cgcggggcaa cacgctcaag caccacatgc
     1201 tgatctggtg ctacaacccg atggcgccca cgggcgtcga catcgtgcgc gcgccgatcg
     1261 acatcaacgg ggcgctcgac ccggcgcttt gcctgatggg ctcggccggc aacgtcaacg
     1321 gcggcggcac gcccgacgtc gcggtcgccg gcggggtcgg ctacgcggtg ttcgacggca
     1381 agaaattgct cgaccccaac gtcgccgggc ccgacacgtt cctgtggatc aaacaaacgc
     1441 tggactgctc gtcggcggcg accgggagct cggtgttcga cttcggcggt gacggcagcg
     1501 cggaggtgcc ctacggcgac gagcagcgcc tgcagatcta caagggcagc gacggcgcgg
     1561 tgctgttcga gacctgcaac accagcggga cgctgcgcga attcccgctc gtcgccgacg
     1621 tcgacagcga cggccacgcg cacatcgtcg cggtctccaa cgcgtactcg agcatcaact
     1681 gcgacggcac caagcagcgc gggatccggg tgttcggcga ctcgaagggc aactgggtgc
     1741 gcacccgccg ggtctggaac cagcacgcgt atcacatcac caacgtcaac gaggacgggt
     1801 cgatcccggc cgcagccggt caactggacg acgcccgggc tcaataactt tcgcagaacg
     1861 cgcagcccga gggcgagttc gcggcgcccg acctgatcgt cacgctgcgc ctg
//
LOCUS       DASWNJ010001427         1737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_130038, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001427 DASWNJ010000000
VERSION     DASWNJ010001427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130038"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1737
                     /gene="uvrA"
                     /locus_tag="VGB85_23770"
     CDS             <1..>1737
                     /gene="uvrA"
                     /locus_tag="VGB85_23770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY0137131.1"
                     /translation="EGQRRYVESLSAYARQFLGQMDKPDVDFIEGLSPAVSIDQKSTS
                     RNPRSTVGTITEVYDYLRLLYARAGTPHCPKCGRVIARQQPSQIVDRVLELEEGTRFQ
                     VLAPVVRARKGEYVDLFADLQTKGYSRARVDGVVHSLAEPPKLKKQEKHTIEVVVDRL
                     TVKESAKRRLTDSVETALGLGGGLVVLDFVDKPEDDPERERTFSEHLACLYDDLSFEE
                     LEPRSFSFNSPFGACPECHGLGTKREVDPELVITDEDASFDGGVIAPWSSGHNIEYFG
                     RLVQALGDALGFTTKTPWKKLTASQQKAVLHGHPTEVHVRYRNRYGRERSYYTAYEGV
                     IPFLERRHSEAESESSRERFEGYMREVPCGVCKGARLKPEVLAVTLGERSIADVAAMS
                     IADCATFLRELQLTDRQMAIAERVVKEVNARLGFLLDVGLDYLSLDRAAGTLAGGEAQ
                     RIRLATQIGSGLVGVLYVLDEPSIGLHQRDNRRLIDTLLRLKSLGNTLIVVEHDEDTI
                     QTADWVVDIGPGAGEHGGQVVVSGSVDDLLASEDSITGAYLSGRREIAVPAVRRPRTK
                     GRELTVVGAREH"
BASE COUNT          270 a          650 c          578 g          239 t
ORIGIN      
        1 cgagggccag cgacgctacg tcgagtcgct gtcggcgtac gcccgccagt tcctcggcca
       61 gatggacaag cccgacgtcg acttcatcga gggcctgtcg ccggcggtct cgatcgacca
      121 gaagtcgacc tcgcgcaacc cccgctcgac cgtcggcacg atcaccgagg tctacgacta
      181 cctgcgcctg ctctacgcgc gcgccgggac gccgcactgc ccgaagtgcg gacgggtcat
      241 cgcgcgccag cagccgagcc agatcgtcga ccgggtgctc gagctcgagg agggcacccg
      301 cttccaggtg ctcgcgccgg tggtccgcgc gcgcaagggc gagtacgtcg acctgttcgc
      361 cgacctgcag accaagggct acagccgcgc ccgggtcgac ggcgtcgtgc actcgctcgc
      421 cgagccgccg aagctcaaga agcaggagaa gcacacgatc gaagtggtcg tcgaccgcct
      481 cacggtcaag gagtcggcca agcgccggct caccgactcg gtcgagacgg cgctcgggct
      541 cggcggcggt ctggtcgtgc tcgacttcgt cgacaagccc gaggacgacc ccgagcgcga
      601 gcgcacgttc tccgagcacc tggcctgcct ctacgacgac ctgtcgttcg aggagctcga
      661 gccgcgcagc ttctccttca acagcccgtt cggcgcctgc ccggagtgcc acggcctcgg
      721 caccaagcgc gaggtcgacc ccgagctggt catcaccgac gaggacgcct ccttcgacgg
      781 gggcgtcatc gcgccgtgga gcagcggcca caacatcgag tacttcggcc gcctcgtcca
      841 ggccctcggc gacgccctgg gcttcacgac caagacgccg tggaagaagc tgaccgcctc
      901 gcagcagaag gccgtcctgc acggccaccc gaccgaggtg cacgtccgct accgcaaccg
      961 ctacggccgc gagcgcagct actacaccgc ctacgagggc gtcatcccgt tcctcgagcg
     1021 gcgccactcc gaggccgaga gcgagtcgag ccgggagcgc ttcgagggct acatgcgcga
     1081 ggtcccctgc ggcgtctgca agggcgcccg cctcaagccg gaggtcctcg cggtcacgct
     1141 cggggagcgg tcgatcgccg acgtcgcggc catgtcgatc gccgactgcg cgacgttcct
     1201 gcgcgagctg cagctgaccg accgccagat ggcgatcgcc gagcgggtcg tcaaggaggt
     1261 caacgcgcgg ctgggcttcc tgctcgacgt cggtctcgac tacctctccc tcgaccgcgc
     1321 ggccggcacg ctggccggtg gcgaggccca gcgcatccgc ctcgcgaccc agatcggctc
     1381 cgggctcgtc ggcgtgctct acgtcctcga cgagccgtcg atcggtctgc accagcgcga
     1441 caaccgccgc ctcatcgaca cgctgctgcg gctgaagtcc ctgggcaaca cgctcatcgt
     1501 cgtggagcac gacgaggaca ccatccagac ggccgactgg gtcgtcgaca tcggtccggg
     1561 ggccggcgag cacggcggtc aggtcgtcgt gtccggctcg gtggacgacc tgctcgcctc
     1621 cgaggactcg atcaccggcg cctacctgtc cggccggcgc gagatcgcgg tcccggccgt
     1681 acgccgcccg cgtaccaagg gccgcgagct caccgtcgtc ggtgcccgcg agcacaa
//
LOCUS       DASWNJ010001428         7562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_337693, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001428 DASWNJ010000000
VERSION     DASWNJ010001428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7562
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337693"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VGB85_23775"
     CDS             <1..715
                     /locus_tag="VGB85_23775"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0137132.1"
                     /translation="HGQPEQAQRVFSEIVEFDPRSPGSRRLLGDIFFRHHWFDRAYRQ
                     YEDLVEMTPDDALAIIRLARAAAGQGRTDEALRILRKVAAGEGRPGADDPRRFARLHA
                     AVYLAGLLADNDAKTPAASVARELGRLQLFDAPATWTLLVWDDLGARLTLTSDPPQPL
                     AGDAIDAGATGLFALQAPASGLPPMTVRHQGLVLDREVAFTAFTVTWDGKEFKVERRQ
                     ATIAAGSPGEGQATLEPPP"
     gene            complement(717..1364)
                     /locus_tag="VGB85_23780"
     CDS             complement(717..1364)
                     /locus_tag="VGB85_23780"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0137133.1"
                     /translation="MLVLHYHPLSSYCMKALIALYEHEVRFTPKLVDLSDPAQRAELA
                     QIWPMIKFPVLRDEASDRTIPEATIIIEYLEQYHPGPAPLIPRDPALAWQTRLRDRFY
                     DLHVQSPMQKIVGDRLRPADRHDPHGVEEAISSLRGACAFVDGEMATRTWANGDAFSM
                     ADCAAAPALFYADKVLPFGDQNFNNARAYLGRLMERPSFARVLAEAQPYFAMFPA"
     gene            1425..2534
                     /locus_tag="VGB85_23785"
     CDS             1425..2534
                     /locus_tag="VGB85_23785"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0137134.1"
                     /translation="MIREALLPLVLLAACDDASGPPATTGEESSSTGSGDETSTGTGG
                     EDIDGPACASARWLLVEPRSDDISAIAVDSRGHVRFAAPIVPNEYRIVDIDAAGAIAA
                     DPRFDSEAALVRFAGVTPDNGILVRFSDRGETDPRSWLRKFDVAGAQVWQTDLGPYPD
                     ANLGATFAAPDGSTIVGVQVVSAALYTIRKYDPAGALVWEVPVSDAYLFPNSINAAGF
                     IAAAQPFSPRLMVLRPDGSTLWDLAFDVPGFDRARIDVAGNIVATSSDGNLMRFSADG
                     ALLWEKTPADLPVALSSISDWTINQSGEIAIIGTSKATHRDTAFKLSPAGELASTVTC
                     ERVFISQVVLDEAGALYLGGSHFRNGAYDAVMAAF"
     gene            complement(2507..3964)
                     /locus_tag="VGB85_23790"
     CDS             complement(2507..3964)
                     /locus_tag="VGB85_23790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137135.1"
                     /translation="MEDSAEPADMVAMARTGPARTLEPAAAAEVLRGALGEHRGDLTI
                     ADAAARAGLALRDAELGLHALLRAQRGHLSVTASGELLFRFPEGLQRQHTTAMRIARA
                     VGGGALAVARWSARIALTVFLLGYSVLFVLVMFVGAIALAFAAESEDPAVAIGYLLWH
                     VVELLGDALYWSVHPLRAPDELDDDAAGRQPRSFYQRVNGFFLGPPRRAHDPKAAAKL
                     LTAEIRFRRGRIGLSDVVRVTGLAPEPASALVSRLLLDYDGTVDVTDDGAIVYRFPAL
                     QPSVDAPVPTAAPAIWRRVRELPAFTGNTLGSNFSIVALTAFVAGFGYLGVWLGLPIW
                     AAEIPLWGSVALLAMVLLRIPGHIARRRADRAENGRRALLRLAYDGACERRGLEIKEF
                     AAAWHEATDTAIDDKQLQAMLLEIGGDLVIDDDGRTSWRFPTIELELGALARVRALVQ
                     TSEREVGAVEFTSLPAEDDEIRALTPAQNAAITAS"
     gene            4133..5377
                     /gene="icd"
                     /locus_tag="VGB85_23795"
     CDS             4133..5377
                     /gene="icd"
                     /locus_tag="VGB85_23795"
                     /inference="COORDINATES: protein motif:HMM:NF005425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEY0137136.1"
                     /translation="MAQAITMTPQGLQVPNNPIIPFIEGDGIGPDIWAASVRVFDAAV
                     EKAYGGERKIEWREVLAGEKAFEKTGNWLPEETLTVFRELLVGIKGPLTTPVGGGIRS
                     LNVALRQELDLYVCLRPVRWFEGVPSPVKRPQDTNMVIFRENTEDIYAGIEFQAGTPD
                     GDKFRDLLKENFPKPFTKVRFPNTASFGIKPVSQEGTERLVRAALEYTVANKLPSLTL
                     IHKGNIMKFTEGSFRDWGYALAQSEYGAKDLDGGPWQVFEKNGHKVVVKDAIADAFLQ
                     QILTRPKEYSVLATLNLNGDYISDALAACVGGIGIAPGGNINYTSGHAIFEATHGTAP
                     KYAGQDKVNPGSVILSGEMMFRYLGWNEAADLIIQGIEQAITAKTVTYDFARMMEGAR
                     EVKCSEFGDAIIQHMGGNKSAA"
     gene            complement(5451..5963)
                     /locus_tag="VGB85_23800"
     CDS             complement(5451..5963)
                     /locus_tag="VGB85_23800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137137.1"
                     /translation="MLRPAPLLLALLFALPACSRATPGPAGPADTAGGERACTEIGCE
                     NGLKITVKKATPWLPGNYVFNFDIDGKAVECKGALPLQACDAGPSLSCTPDAVVQIGE
                     SGCALPPEQHGFSDITVRGEPQVVKLKVLLGDKPLNAADVTPNYVTTQPNGAGCEPTC
                     RNAGSEVEIP"
     gene            6094..7386
                     /locus_tag="VGB85_23805"
     CDS             6094..7386
                     /locus_tag="VGB85_23805"
                     /inference="COORDINATES: protein motif:HMM:NF025670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137138.1"
                     /translation="MRRLGPVLGASLLAWPASAAADVGPERFLSEHANLTFGAALAGD
                     GSRLAVGAPGANDVGFDAGAVYVYGRDRGEWVLRQRLEPDTGPYQHFGSALALDGDTL
                     LVAGEAPLATGGATGVAHLYEVEHGELVARERWVLDEQASLGYLGLAVAVDGDTLAFG
                     GYLAQTTATVPGGRVRVMRRAGGQWVEEADLGGGELLGATLAIDGDWLFVQADYGERV
                     AIYRRDAGGVWQAQPDLELAGAEGPIEAATIVSDDGALVIADRVSPVLHFYGPGAGGW
                     QRVDTRSYVGDMYSEGYGEALAIGDGRVAYTSTVQTYEPAFSHVDTIHVLRRDGDAWV
                     DDGEVERDARSDVHEAFGARLALAGGTLLAGAPEYGDPGYERSGQVHVFARDGGWEAR
                     QILGPDDVLGGDGCGCRGGVAPGWYALLLVWPRRRRRP"
     gene            7383..>7562
                     /locus_tag="VGB85_23810"
     CDS             7383..>7562
                     /locus_tag="VGB85_23810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137139.1"
                     /translation="MKSVICASLLALAGLLAARPASACSVISPRPGLELAVDEPRITE
                     DGVLLVRGEAFTVDVD"
BASE COUNT         1105 a         2706 c         2669 g         1082 t
ORIGIN      
        1 tcatggtcag cccgagcagg cccagcgcgt gttctccgag atcgtcgagt tcgacccgcg
       61 ctctcccggc agccgccgcc tgctcggcga catcttcttt cgccatcact ggttcgaccg
      121 cgcataccgg caatacgagg acctcgtcga gatgaccccc gacgacgcgc tggcgatcat
      181 tcgcctcgcc cgcgccgcgg ccggccaggg ccgcaccgac gaggccctgc ggatcctgcg
      241 taaagtggca gcaggcgagg gccgccccgg cgccgacgac ccgcgccgct tcgcccgcct
      301 gcacgccgcg gtttacctcg ccggcctgct cgccgacaat gacgccaaga cgccggccgc
      361 cagcgtcgcc cgcgagctcg gccgcctgca actcttcgac gcccccgcga cctggacctt
      421 gctggtctgg gacgacctcg gggcgcgcct gaccctgacc tcggatcctc cgcagccgct
      481 cgccggcgac gcgatcgacg ccggcgcgac cggcctgttc gcgttgcagg cacccgcgag
      541 cggcctgccg ccgatgaccg tccgccacca gggcctggtg ctcgaccgcg aggtcgcgtt
      601 cacagcgttc accgtcacgt gggacggcaa ggagttcaag gtcgagcgcc gccaggcgac
      661 gatcgcggcc ggcagccccg gtgaggggca ggccacgctc gagcccccac cgtgagctac
      721 gccgggaaca tggcgaagta gggctgggcc tccgcgagca cccgggcgaa ggatggtcgt
      781 tccatcagac gcccgaggta cgcgcgcgca ttattaaaat tctggtcgcc gaacggcagc
      841 accttgtccg cgtagaacaa cgccggcgcg gccgcgcagt cggccatcga gaaggcgtcg
      901 ccgttggccc aggtgcgcgt cgccatttcg ccgtcgacga acgcacaggc gcctcgcagc
      961 gagctgatcg cctcctcgac gccgtgcggg tcgtggcgat cggcgggccg cagccggtcg
     1021 ccgacgatct tctgcatcgg cgactgaacg tggagatcat aaaaacggtc gcgcaggcgc
     1081 gtctgccagg cgagcgccgg atctctcggg atcagcggcg cggggcccgg gtggtactgc
     1141 tcgagatact cgatgatgat cgtcgcctcg gggatcgtgc ggtcgctggc ctcgtcgcgc
     1201 aggaccggga acttgatcat cggccagatc tgcgcgagct cggcccgctg ggccgggtcc
     1261 gagaggtcga cgagcttcgg cgtgaagcgg acctcgtgct cgtacagggc gatcagcgcc
     1321 ttcatgcagt acgacgagag agggtggtaa tggaggacga gcatgacatg taggagtgta
     1381 tccctgccgg cagaagctct gcaaatgtgt cacgctgccg atcgatgatc cgcgaggccc
     1441 tgctccccct ggtgttgctc gcggcctgtg acgacgcctc ggggccgccc gcgaccaccg
     1501 gtgaggagag cagctcgacg ggcagcggcg acgagacctc gaccggcacc ggcggtgagg
     1561 acatcgatgg gccggcatgt gccagcgctc gctggctgct cgtcgagccg cgcagcgacg
     1621 acatctcggc gatcgccgtc gactcgcgcg ggcacgtccg cttcgccgcg ccgatcgtgc
     1681 cgaacgagta tcgtatcgtc gacatcgacg ccgcgggcgc gatcgcggcg gacccccggt
     1741 tcgacagcga ggccgcgctg gtgcgcttcg ccggcgtcac cccggacaac ggcatcctcg
     1801 tccgcttcag cgatcgcggc gagaccgacc cgcgcagctg gctgcgcaag ttcgacgtcg
     1861 ccggcgcgca ggtgtggcag accgacctcg gcccctaccc ggacgccaac ctcggtgcca
     1921 ccttcgccgc gccggacggc tcgacgatcg tcggcgtgca ggtcgtgtcc gccgccctgt
     1981 atacgatccg caagtacgac cccgcgggcg ccctggtgtg ggaggtcccg gtgtcggacg
     2041 cctacctgtt cccgaattcg atcaacgccg ccggcttcat cgccgcggcc cagccgttca
     2101 gtccgcgcct catggtgctc aggccggacg gcagcacgct gtgggacctc gccttcgacg
     2161 tgcccggctt cgacagggcg aggatcgacg tcgccggcaa catcgtcgcc acctcctcgg
     2221 acggaaatct catgcgcttc tccgccgacg gcgcgttgtt gtgggagaag acgcccgccg
     2281 acttgccggt cgcgctgtcc agcatctccg actggacgat caatcaaagc ggcgagatcg
     2341 ccatcatcgg tacgagcaag gcgacccatc gtgacaccgc gttcaagctg agcccggcgg
     2401 gcgagctggc gagcaccgtc acctgcgagc gcgtcttcat ctcgcaggtc gtcctcgacg
     2461 aggccggcgc gctctacctc ggcggctctc acttccgcaa cggggcctac gacgcggtga
     2521 tggccgcgtt ctgagcgggc gtgagcgccc ggatctcgtc gtcctcggcc ggcagcgacg
     2581 tgaactcgac ggcaccgacc tcgcgctcgc tggtctgcac cagcgcgcgc acgcgggcca
     2641 gcgcgccgag ctcgagctcg atcgtcggga accgccagct ggtgcggccg tcgtcgtcga
     2701 tgacgaggtc gccgccgatc tcgagcaaca tcgcctgcaa ttgcttgtca tcgatcgccg
     2761 tgtcggtcgc ctcgtgccag gccgcggcga actctttgat ctcgagcccg cggcgctcgc
     2821 aggcgccgtc gtacgcgagc cgcagcagcg cgcggcggcc gttctcggcg cggtcggccc
     2881 ggcggcgggc gatgtgcccg gggatccgca gcagcaccat cgccagcagc gcgacgctgc
     2941 cccacagcgg gatctcggcg gcccagatcg gcaggccgag ccacacgccg aggtagccga
     3001 agccggcgac gaacgcggtc agcgcgacga tgctgaagtt gctgccgaga gtgttgccgg
     3061 tgaacgcggg cagctcgcgg acgcggcgcc agatcgccgg cgcggcggtc ggcaccgggg
     3121 cgtcgacgct cggctgcagc gcggggaagc gatagacgat cgcgccgtcg tcggtgacgt
     3181 cgacggtgcc gtcgtagtcg agcagcaggc gggagaccag cgcgctcgcg ggctcgggcg
     3241 cgaggccggt gacgcggacg acgtcggaga ggccgatgcg acccctgcga aaccggatct
     3301 cggccgtcaa cagcttcgcg gcggccttgg ggtcgtgcgc gcgtctcggc gggccgagga
     3361 agaagccgtt gacgcgctga taaaagctgc gcggctgtcg gcctgccgcg tcgtcgtcga
     3421 gctcgtcggg cgcgcgcagc gggtgcacgc tccagtacag cgcgtcgccc agcagctcga
     3481 cgacgtgcca cagcaggtag ccgatcgcca ccgccgggtc ctcgctctcg gcggcgaagg
     3541 ccagcgcgat cgcgccgacg aacatcacga gcacgaacag caccgagtag ccgagcagga
     3601 acaccgtcag cgcgatcctt gcggaccagc gggccaccgc gagcgcgccg ccgccgaccg
     3661 cccgcgcgat ccgcatcgcc gtggtgtgct gcctctgcag accctcgggg aagcgaaaca
     3721 gcagctcgcc gctggcggtg accgacaggt ggccccgctg cgcccgcaga agtgcatgca
     3781 ggccgagctc ggcgtctcgc agggccaggc cggcgcgcgc ggccgcgtcg gcgatcgtca
     3841 ggtcaccgcg gtgctcgccg agcgccccgc gtagtacctc ggcggccgcg gccggctcga
     3901 gcgtccgggc cgggccggtg cgagccatgg cgaccatgtc cgccggttcg gcgctgtcct
     3961 ccatccgcat cagcttacgc gccggggctg ccgcgctgac gctcgccgtg gtgtttcccg
     4021 gtgtcttttc gcgccctgcg cgctggcccg cgcgagggct cacagggcca ccacggcacg
     4081 tgtttcgcgg gccccggcag ggctggcgcg cgctgtggta tacggcccga cgatggctca
     4141 agcgatcacg atgaccccgc aggggctcca agtccccaac aaccccatca tccccttcat
     4201 cgagggcgac gggatcggcc ccgacatctg ggccgcctcg gtccgtgtct tcgacgctgc
     4261 ggtcgagaag gcctacggcg gcgagcgcaa gatcgagtgg cgcgaggtgc tggccggcga
     4321 gaaggccttc gagaagaccg gcaactggct gcccgaggag acgctgaccg tctttcgcga
     4381 gctgctcgtc gggatcaagg gcccgctgac cacgcccgtc ggcggcggca tccgcagcct
     4441 caacgtcgcg ctgcgccagg agctcgacct ctacgtgtgc ctgcgtccgg tccgctggtt
     4501 cgagggcgtg ccgagcccgg tcaagcgccc gcaggacacc aacatggtga tcttccgcga
     4561 gaacaccgag gacatctacg ccggcatcga gttccaggcc ggcaccccgg acggcgacaa
     4621 gttccgcgac ctgctcaagg agaacttccc caagccgttc accaaggtcc gcttcccgaa
     4681 cacggcgagc ttcggcatca agccggtctc gcaggagggc accgagcgcc tggtccgcgc
     4741 cgcgctcgag tacacggtcg ccaacaagct cccgagcctg acgctgatcc acaagggcaa
     4801 catcatgaag ttcacggagg gctccttccg tgactggggc tacgccctcg cgcagtcgga
     4861 gtacggcgcc aaggacctcg acggtggccc gtggcaggtg ttcgagaaga acggccacaa
     4921 ggtcgtcgtc aaggacgcga tcgccgacgc cttcctgcag cagatcctga cccgcccgaa
     4981 ggagtactcg gtgctcgcca ccttgaacct caacggcgac tacatctccg acgcgctcgc
     5041 ggcgtgcgtc ggcggcatcg gcatcgcgcc gggcggcaac atcaactaca cctccggcca
     5101 cgcgatcttc gaggccaccc acggcacggc gcccaagtac gccggccagg acaaggtcaa
     5161 cccgggctcg gtgatcctct ccggcgagat gatgttccgc tacctcggct ggaacgaggc
     5221 cgccgacctg atcatccagg gcatcgagca ggcgatcacc gccaagacgg tcacctacga
     5281 cttcgcccgc atgatggaag gcgcccgcga ggtcaagtgc tccgagttcg gcgacgcgat
     5341 catccagcac atgggcggca acaagtccgc cgcctgatcg tcgatcgctc gttgcgatct
     5401 gacacgacgg gccgcgctcc ccggagcgac ggcccgtcgg caattttatc tcaggggatc
     5461 tcgacctcgg agcccgcgtt gcggcaggtc ggctcgcagc cggccccgtt gggctgcgtg
     5521 gtcacgtagt tcggcgtcac gtcggcggcg ttgagcggct tgtcgccgag cagcaccttg
     5581 agcttgacca cctgcggctc gccgcgcacc gtgatgtccg agaagccgtg ctgctcaggc
     5641 ggcaaggcgc agccggactc gccgatctgc accaccgcgt ccggtgtgca gctcagcgag
     5701 gggccggcgt cgcaggcctg cagcggcagg gcccccttgc actcgaccgc cttgccgtcg
     5761 atgtcgaagt tgaacacgta gttgccgggc agccacggcg tcgccttctt gaccgtgatc
     5821 ttcagcccgt tctcgcagcc gatctcggtg caggcccgct caccgcccgc ggtgtcggcc
     5881 gggccggccg ggccgggcgt ggccctggaa caggcaggga gggcgaacag cagggccagg
     5941 aggagcgggg cagggcgcaa catgggcagc agcgtgccac gatccccgcg cggcgcaagc
     6001 gcctcgcccc tcgttggccc cgcggaaagt ggggcaagtg gcccccaaat ttaccgctgg
     6061 cggacccggc attgtcgcgg cttatactgc ttcatgcgtc gcctcggacc tgtccttggg
     6121 gccagcttgc tcgcgtggcc tgcgagcgcc gccgcggacg tcgggcccga gcgcttcctg
     6181 agcgagcacg ccaacctgac cttcggcgcc gcgctggccg gcgatggctc gcggctcgcg
     6241 gtcggtgctc cgggcgccaa cgacgtcggc ttcgacgccg gcgccgtgta cgtgtacggg
     6301 cgggaccgcg gcgagtgggt cctgcgccag cgcctggagc ccgacaccgg gccgtaccag
     6361 cacttcggca gcgcgctggc cctcgacggc gacactctgt tggtcgccgg cgaggccccg
     6421 ctcgccaccg gcggagcgac cggtgtggcc cacctgtacg aggtcgagca cggcgagctc
     6481 gtcgcgcgcg agcgctgggt cctcgacgag caggcgagcc tcggctacct cggcctcgcg
     6541 gtcgcggtcg acggcgacac gctggccttt ggcggctacc tcgcgcagac gaccgcgacc
     6601 gtgcccggcg ggcgggtccg ggtgatgcga cgcgcgggtg ggcagtgggt cgaggaggcg
     6661 gacctcggag gcggggagct gctcggcgcg acgctggcga tcgacggcga ctggctgttc
     6721 gtgcaggccg actacggcga gcgtgtcgcg atctaccggc gcgacgcggg cggggtgtgg
     6781 caggcgcagc ccgacctcga gctggccggc gccgagggcc cgatcgaggc ggccacgatc
     6841 gtcagcgacg acggcgcgct ggtgatcgcc gaccgcgtat ctccggtcct ccacttttat
     6901 ggcccgggcg ccggcggctg gcagcgcgtg gacacgcgga gctatgtcgg cgacatgtac
     6961 tccgagggct acggcgaggc actggcgatc ggcgacgggc gcgtcgcgta caccagcacc
     7021 gtccagacct acgagcctgc attctcgcac gtcgacacga tccacgtgct gcggcgcgac
     7081 ggggacgcgt gggtcgacga cggcgaggtc gagcgcgacg ccaggagcga cgtccacgag
     7141 gccttcggcg cccggctggc gctcgccggc ggcacgctgc tcgcgggcgc gcccgagtac
     7201 ggcgaccccg ggtacgagcg gtcgggccag gtccacgtgt tcgcccgcga cggcggctgg
     7261 gaggcgcgcc agatcctcgg ccccgacgac gtgctcggcg gcgacggctg tggctgtcgc
     7321 ggcggcgtcg cgccgggctg gtacgcgctg ttgctggtgt ggccgcgacg acgaaggaga
     7381 ccatgaagtc agtcatctgc gcatcgctgc tcgcgctggc gggcttgctc gcggcccgtc
     7441 cggcctcggc ctgctcggtc atctcgccgc gcccgggcct cgagctcgcg gtcgacgagc
     7501 cacggatcac cgaggacggt gtgctgctgg tccgcggcga ggccttcacc gtcgacgtcg
     7561 ac
//
LOCUS       DASWNJ010001429         5503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_202607, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001429 DASWNJ010000000
VERSION     DASWNJ010001429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202607"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..650
                     /locus_tag="VGB85_23815"
     CDS             243..650
                     /locus_tag="VGB85_23815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137140.1"
                     /translation="MKFLSPKLHGYIDYLTSAAFMMIPVLFMVQGVPRAVMGFYAIGG
                     ALLLVSLITRYPLGALKIVPFPVHGAFELIGAPLVVAYPWLAGFADVPAARNTYIVAG
                     VALFLVWLVTDYKAAEAPHHSESAATGPVASHR"
     gene            719..901
                     /locus_tag="VGB85_23820"
     CDS             719..901
                     /locus_tag="VGB85_23820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137141.1"
                     /translation="MSKHELPRSVQEQPGAGVKNSSNPQYPTNDMPHSNEKNPAMPAH
                     QDRRGSKGGSPGGNKP"
     gene            complement(978..2645)
                     /locus_tag="VGB85_23825"
     CDS             complement(978..2645)
                     /locus_tag="VGB85_23825"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0137142.1"
                     /translation="MTASATLRRAPLVFAALLLACHARPANHGPAVASAALAGDWIGG
                     YKIGKRYSLFALHLPSRAAERPSFDLPAEGVRAATLTDYLPAGDALRFMLPGGRSKVA
                     LQGRLEHESLIGAARVEGEEGSFEMRRMTPVEPEALREYMGLYDAGAGHLIAFERMPE
                     LAQLHFADFFSGRFNAIFPDGKDRFFAGPALLVPAPAVMVLTFNRDPQGAITGVTFQQ
                     DGEPDVVAVRVPTREAALEFQSGPLTLAGTLLAPATPGPHPAVVLVPNERGAPRDAYR
                     KDADFLVSQGFAALIYDRRGVGASQGEASQTPIPALADDALAAVELLRGRADIERGRV
                     GVWGHSQGGWVATLAGARSPAVAFVINVSTPTLPAAEQEIYRIEHAMRVDGFSEPQIL
                     EAVNYQRLLMDWVRDGTGRDTLISVHRAAASAPWASYVSLPPSPLPDQPRAATREFYR
                     YDPIPAIAELRCPALFVYGKADSFVPVERSVPLIKTAMVRRSHPDTQLKVLPRTGHGM
                     YETDLDSRDALPLSRRHGQGYWQLLATWLSKARVSAPPEPLAPAPGK"
     gene            complement(2713..4164)
                     /locus_tag="VGB85_23830"
     CDS             complement(2713..4164)
                     /locus_tag="VGB85_23830"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0137143.1"
                     /translation="MAREGPKLSTIRTDIPARMDRLPWSRWHWRVLVGLGTVWILDGL
                     AVTIVGAIGGRITEAGSGLALTTTQVGAAGAAYILGACAGALYFGRMADRIGRKRLFM
                     LTLLVFLVGSLLTGLATHYVWFMVCRVVTGAGVGGEYSAIHSAVDELIPARVRGVVDL
                     VIGGSYWLGTILGSLASLVLLDAALFPVNVGWRLCFALTAVMGLAIVLVRRHVPESPR
                     WLLLHGYEDEAERVTADIERQIVAETGRPLPAPERTMRVKQRAPMGIAEIAAVVFRMY
                     PRRTVVGLALFTGQAFLYNAIFFTYALVLTEVYGVDGGRVGLYLLPFAVGNFLGPLVL
                     GRLFDTVGRKPMIAGTYILSGLLLLITGMLFKWGMLDATTQTVAWCVVFFFASAGASA
                     AYLTVSEIFPMETRAMAIAFFYATGTIVGGFGGPLLFGALIATGDPGQILIGYAIGAV
                     MMIAGGVVQAIMGVEAAGRDLEAIAPPLSLVED"
     gene            4817..5290
                     /locus_tag="VGB85_23835"
     CDS             4817..5290
                     /locus_tag="VGB85_23835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137144.1"
                     /translation="MLATITSLVATLAFAPATLAAPTTPTEGASCSWMNCWNDTGDHD
                     DDDDNGGGDDDDGGDLDLDLDACLDLDLDIDLDCEVEVDAECHADCEGGVHAYCEAAC
                     GDDDWSDEQWASCEAQVEAECHANCEIDGSVFCEGDIYVGADVCLDIGLDLDLDL"
BASE COUNT          901 a         2099 c         1720 g          783 t
ORIGIN      
        1 ccggctccgg cgcgggcggc ggctccggct ccggcatggg cggctccggc ggctcgggct
       61 ccggcatgac cggctcgacc ggctcgggcg cgggcggcac tggcgccacc ggcacgggca
      121 cctcgcgtgg cggctcgggc tcgggctcag gctccccgcg ctgatctctg cgcttcgaat
      181 caatgaggac aggcgtcgcc tgtcctcgcg tttgaccccg acacgaccat gaggaggccg
      241 cgatgaaatt cctgtccccg aagctccacg gatacatcga ttatcttacg agcgccgcgt
      301 tcatgatgat cccggtattg ttcatggttc agggggtccc ccgcgcggtg atgggcttct
      361 acgcgatcgg cggcgcgctg ctgctggtct cgctgatcac ccgatatccg ctcggcgcgc
      421 tcaagatcgt gccgttcccg gtgcacggcg ccttcgagct gatcggcgcg cccctggtgg
      481 tcgcataccc gtggctcgcc ggttttgccg atgtgccggc cgcgcgtaac acctacatcg
      541 tcgccggcgt cgcgctgttc ctcgtgtggc tggtgaccga ctacaaggcc gccgaggcgc
      601 cccaccattc cgagtccgcg gcgaccggcc ccgtcgcgtc ccatcgctga atcctcgctg
      661 gcgctccgcc aggcttcgat ccacggttga cgctcaccca cgaaaggttt caaacgacat
      721 gagcaagcat gaacttcccc gttcggtcca ggagcagccc ggtgctggcg tgaagaactc
      781 cagcaacccc cagtacccga ccaacgacat gccgcactcc aacgagaaga accccgccat
      841 gccggcccat caggaccgtc gaggcagcaa gggcggcagc cccggcggca acaagccctg
      901 agataacggg cgaccgctgc aacaatgcgg ccgcgagtcc cggactcgcc ggccgcttta
      961 ttctttgaag atatcgctca cttgccaggc gcgggcgcga gcggctccgg cggcgcgctg
     1021 acccgggcct ttgacagcca cgtagcgagc aattgccagt acccctgccc gtgtcggcgc
     1081 gacagcggga gcgcgtcgcg gctgtcgagg tcggtctcgt acatgccatg gccggtgcgc
     1141 ggcaggacct tgagctgcgt gtccgggtgg ctgcggcgca ccatcgcggt tttaatcaat
     1201 ggcacgctgc gctcgaccgg cacgaacgag tcggccttgc cgtacacgaa cagcgccggg
     1261 cagcgcagct ccgcgatcgc agggatcggg tcgtatcgat aaaactcgcg ggtcgccgcg
     1321 cgcggctggt ccggcaatgg tgacggcggc aggctgacat acgaggccca cggcgccgac
     1381 gcggcggccc ggtgcaccga gatcagcgtg tcgcggccgg tgccgtcgcg cacccagtcc
     1441 atcagcaggc gctgatagtt caccgcctcg aggatctgcg gctcggagaa gccgtcgacg
     1501 cgcatcgcgt gctcgatgcg atagatctcc tgctccgccg cgggcaaggt cggcgtcgag
     1561 acgttgatga cgaacgcgac cgccggtgag cgcgcgccgg ccagcgtcgc cacccagccg
     1621 ccctggctgt gcccccacac gccgacgcgc ccgcgctcga tgtccgcgcg gccgcgcagc
     1681 agctccaccg ccgccagcgc gtcgtcagcg agcgccggga tcggcgtctg cgaggcctcg
     1741 ccctgcgacg ccccgacccc gcgccggtcg tagatcagcg ccgcgaagcc ctgcgagacc
     1801 aggaagtcag cgtccttgcg gtacgcgtca cgcggggccc cgcgctcgtt cggcaccagc
     1861 acgaccgccg ggtgcggccc cggggtcgcc ggggccagca gcgtgccggc gagcgtcagc
     1921 gggccgctct ggaactcgag cgccgcctcg cgggtcggca cgcgcaccgc gacgacatcg
     1981 ggctcgccgt cctgttgaaa cgtgacgccg gtgatcgccc cttgcgggtc gcggttgaag
     2041 gtcaggacca tcaccgccgg cgccggcacc agcagcgccg gcccggcgaa gaagcggtcc
     2101 ttgccgtccg ggaagatcgc gttgaagcgg cccgagaaga agtccgcgaa gtgcagctgc
     2161 gcgagctccg gcatccgctc gaacgcgatc aggtgccccg cgcccgcgtc gtacaggccc
     2221 atgtactcgc gcagcgcctc cggctcgacc ggcgtcatcc gccgcatctc gaagctgccc
     2281 tcctcaccct ccacccgcgc cgcaccgatc aacgactcgt gctcgagccg accttgcaac
     2341 gccaccttgc tgcgcccccc tggaagcatg aagcgcaggg cgtcgcccgc cgggagatag
     2401 tcggtcagcg tcgccgcgcg cacgccctcg gccggcaggt cgaagctcgg ccgctcggcc
     2461 gcgcggctcg gtaagtgcag cgcgaacagg ctgtagcgct tgccgatctt gtagccgccg
     2521 atccagtcgc cggcgagcgc cgccgaggcg acggccggcc cgtggttcgc cggtcgcgca
     2581 tggcaggcga gcagcagcgc ggcgaacacc agcggcgcac ggcggagggt ggcggacgcg
     2641 gtcatgatca cgagcgcgcc gagtttagcg caatctcccg ccgcgtggcc cgcggtgcgc
     2701 gcgccactgc catcaatcct ccacgagcga cagtgggggt gcgatggcct cgaggtcacg
     2761 cccggcggcc tcgaccccca tgatcgcctg gaccaccccg ccagcgatca tcatcacggc
     2821 cccgatcgca tagccgatca ggatctgccc cgggtcgccg gtcgcgatga gcgcgccgaa
     2881 cagcagaggt ccgccgaagc cgccgacgat cgtgccggtc gcataaaaga acgcgatcgc
     2941 catcgcccgc gtctccatcg ggaagatctc gctgaccgtc aggtacgccg cgctcgcccc
     3001 cgccgacgcg aagaagaaca ccacgcacca cgcgaccgtc tgcgtggtcg cgtcgagcat
     3061 cccccatttg aagagcatcc ccgtgatcaa cagcagcaac cccgaaagga tatacgtccc
     3121 ggcgatcatc ggcttgcggc cgaccgtgtc gaacagccgc ccgagcacca gcggcccgag
     3181 gaaattcccg accgcgaacg gcagcagata cagcccgacc cgcccgccgt cgaccccata
     3241 tacctccgtc agcaccagcg cgtaggtgaa gaagatcgcg ttgtaaagaa acgcctgccc
     3301 ggtgaacagc gccagcccga cgaccgtgcg ccgcggatac atgcgaaata ccaccgcggc
     3361 gatctcggcg atgcccatcg gcgcgcgctg cttgacccgc atcgtgcgct ccggcgccgg
     3421 cagcggccgc ccggtctcgg cgacgatctg gcgctcgatg tcggccgtga cccgctccgc
     3481 ctcgtcctcg tatccgtgca gcagcaacca ccgcgggctc tccggcacgt gccggcgcac
     3541 cagcacgatc gccaggccca tcaccgccgt cagcgcgaag cacagccgcc agccgacgtt
     3601 caccgggaac agcgccgcgt cgagcagcac cagcgacgcc agcgacccaa ggatcgtgcc
     3661 gagccagtac gacccgccga tcaccaggtc gacgaccccg cgcacgcgcg ccgggatcag
     3721 ctcgtcgacc gccgagtgga tcgccgaata ttcgccgccg acccccgcgc cggtgacgac
     3781 cctgcaaacc atgaaccaca cataatgcgt cgccagcccg gtcagcaggc tgccgacgag
     3841 gaacaccagc agcgtcagca tgaacagccg cttgcgcccg atgcggtcgg ccatccgccc
     3901 gaagtacagc gccccggcgc acgccccgag gatgtacgcc gccccggccg ccccgacctg
     3961 cgtggtcgtc agcgccagcc cgctcccggc ctcggtgatc cgcccgccga tcgcaccgac
     4021 gatcgtcacc gccagcccgt cgaggatcca caccgtcccg agcccgacca gcacgcgcca
     4081 gtgccaccgc gaccacggca gccggtccat ccgcgccggg atgtcggtcc ggatcgtgct
     4141 cagcttcggc ccctcgcgcg ccatgaccac ctcttcgcaa acctccgcgc gccggcccac
     4201 caccacgcga caattcccct cgcacgcccc ccgaccccac ccccgccaat cttttaaaag
     4261 atcctgcgca accgccgcga cgcttcgtta tcagtcgcag caatgtcccg tggcgcccgg
     4321 atcgcactcg cacaactccc gcgcgcaggt ccagcaatcc cgacatacgc ccggcccctt
     4381 cgccccgagc ccggccagat cgcatcaaat cgccgcggct cgcgccggtc atgtgcatat
     4441 tatgtttgca tttcaacaac ttacggctca tcagcgacag acgagcgcgt cagccctggc
     4501 gagctatgcg atctttcgcg gcgaacggca cttgccttat cgggtcaatg gacgatttca
     4561 tgtccaccca tcctgccgca ttgccggtga tcgtcgcgtc tgtaaaaaat cgtgaattcg
     4621 ggcccacgcc cgcgcacacg ggcgattatc gggtgatcca cgattctgaa cagtgtcgcc
     4681 ggaatcaccg cactcccgtg tatacgttgc ggggcgcagc cccccacaca tactcacctc
     4741 atcgagccgc cgcgaatgcc tccggcggcc accctcaccc ctcccgtctt cgtccgcgaa
     4801 gagaagaagg atccccatgc tcgccaccat cacctccctc gtcgccaccc tcgccttcgc
     4861 ccccgccacg ctcgccgcgc cgacgacccc gaccgaaggg gcctcgtgct cgtggatgaa
     4921 ctgctggaac gacaccggcg atcacgacga cgatgatgac aatggcggtg gcgacgacga
     4981 cgacggtggc gacctcgacc tcgacctcga cgcctgtctc gacctcgacc tcgatatcga
     5041 cctcgattgt gaggtcgagg tcgacgccga gtgccacgcc gactgcgagg gcggggtcca
     5101 cgcctactgc gaggccgcct gcggcgacga tgactggagc gacgagcagt gggcctcgtg
     5161 cgaggcccag gtcgaggccg agtgccacgc caactgcgag atcgacggct cggtcttctg
     5221 cgagggcgac atctacgtcg gcgccgacgt gtgcctcgac atcggcctcg acctcgacct
     5281 cgacctgtaa tcccgcgagc gaacgaggcc cggtgcgccc gggcctcgtc aggtcgatcg
     5341 cgcgagcttg atccggttgg tcacctcgcg cacgccgcgg atctgctcga ccgcatcctc
     5401 gatccgccag cggtcgcggc ggtcctgcac ggtgccggtc agcagcacca cgccgtcgcg
     5461 gacctcgacc tcgacctcgc tcgcgtcgat ctcgtcgtcc cac
//
LOCUS       DASWNJ010001430         1609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_273986, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001430 DASWNJ010000000
VERSION     DASWNJ010001430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273986"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VGB85_23840"
     CDS             complement(<1..881)
                     /locus_tag="VGB85_23840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0137145.1"
                     /translation="MADMAQLAKTRNIGIMAHIDAGKTTTTERILFYTGITYKIGEVH
                     DGAATMDWMEQEQERGITITSAATKCVWNGFDINIIDTPGHVDFTVEVERSLRVLDGA
                     VAVYDGVAGVEPQTEQVWRQAEKYHVPRMCFVNKLDRTGANFFRCVDMMVERLNANPL
                     VLQLPIGAEGDFIGVVDLVTMKALTWRGETKLGEDYAVEPIPADMQEQAEEWRTKLIE
                     TVSENDDEAMEAYLAGEELSLEQLNAAIRRATIAGKVNPVVCGSAFKNKGVQPMLDAV
                     VAYLPSPLDIGATHGTLP"
     gene            complement(988..1458)
                     /gene="rpsG"
                     /locus_tag="VGB85_23845"
     CDS             complement(988..1458)
                     /gene="rpsG"
                     /locus_tag="VGB85_23845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEY0137146.1"
                     /translation="MPRKGPAPKRPLVADPVYQSQLVTQLVNKVLVDGKRSIAEAIVY
                     GALEGARAKSDTDPVVTLKRALDNVKPALEVRSRRVGGATYQVPVEVRPSRSTTLGLR
                     WLIQYSRARREKTMTERLMNELLDASTGLGAAVKRREDTHKMAESNKAFAHYRW"
     gene            complement(1458..>1609)
                     /locus_tag="VGB85_23850"
     CDS             complement(1458..>1609)
                     /locus_tag="VGB85_23850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEY0137147.1"
                     /translation="EHSMVLVRGGRVKDLPGVRYKIIRGSLDTQAVRGRKQARSRYGA
                     KKEKS"
BASE COUNT          210 a          540 c          559 g          300 t
ORIGIN      
        1 ggcagcgtgc cgtgggtggc gccgatgtcc agcggcgacg gcaggtacgc caccaccgcg
       61 tccagcatcg gctgcacgcc cttgttcttg aaggccgagc cacacacgac cgggttcacc
      121 ttgccggcga tggtcgcgcg acggatcgcg gcgttgagct gctccaggga gagctcctcg
      181 ccggcgaggt aggcctccat ggcctcgtcg tcgttctcgg aaacggtctc gatgagcttg
      241 gtccgccact cttcggcctg ctcctgcatg tcggcaggga tcggctcgac ggcgtagtcc
      301 tcgccgagct tggtctcgcc gcgccaggtc agggccttca tcgtgaccag gtcgacgacg
      361 ccgatgaagt cgccctcggc accgatggga agctgcagca ccagcgggtt ggcgttcagc
      421 cgctcgacca tcatgtcgac gcagcggaag aagttcgcac cggtgcggtc gagcttgttg
      481 acgaagcaca tccgcggcac gtggtacttc tcggcctgcc gccagacctg ctcggtctgc
      541 ggctcgacac cggcgacacc gtcgtagacg gcgaccgcac cgtcgagcac ccgcagcgac
      601 cgctccacct cgacggtgaa gtcgacgtgc ccgggggtgt cgatgatgtt gatgtcgaag
      661 ccgttccaga cgcacttcgt cgcggccgac gtgatggtga tgccgcgctc ctgctcctgc
      721 tccatccagt ccatcgtggc cgcgccgtcg tggacctcac cgatcttgta cgtgataccg
      781 gtgtagaaga ggatgcgctc ggtggtggtc gtcttgccgg cgtcgatgtg cgccatgatc
      841 ccgatgttcc gggtcttggc gagctgggcc atgtcggcca tctcttactc ctctttgttt
      901 cgggtccgcg tgcgggttcc gggggtcgcc cgggttgtcc cgccggcagc gggcaccggc
      961 gcccgtcggg cgccggcagg ggtgctgtta ccagcggtag tgcgcgaagg ccttgttcga
     1021 ctcggccatc ttgtgcgtgt cctcgcgccg cttcacggcg gcgccgaggc cggtgctcgc
     1081 gtcgagcagc tcgttcatca ggcgctcggt catcgtcttc tcacgacggg cgcggctgta
     1141 ctggatgagc cagcgcagcc cgagggtggt gctgcgcgag ggacggacct cgaccgggac
     1201 ctggtaggtc gcgccaccga cacggcggct gcggacctcg agggccggct tcacgttgtc
     1261 cagcgcgcgc ttgagcgtga cgaccgggtc ggtgtcgctc ttggcgcggg cgccctcgag
     1321 ggcgccgtag acgatcgcct cggcgatcga gcgcttgccg tcgacgagga ccttgttcac
     1381 cagctgcgtg accagctggg actggtagac cgggtcggcg accagcggcc gcttcggcgc
     1441 ggggcccttg cgaggcatta gctcttctcc ttcttggcgc cgtaacggct gcgggcctgc
     1501 ttgcggccac ggacggcctg cgtgtccagc gagccgcgga tgatcttgta gcgcacgccc
     1561 ggcaggtcct tcacgcgacc accgcgcacg agcaccatcg agtgctcct
//
LOCUS       DASWNJ010001431         4770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_695522, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001431 DASWNJ010000000
VERSION     DASWNJ010001431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695522"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /gene="amrB"
                     /locus_tag="VGB85_23855"
     CDS             <1..625
                     /gene="amrB"
                     /locus_tag="VGB85_23855"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEY0137148.1"
                     /translation="AYKQLMAMDPKPRRVVLLGPAHTVAFRGLALPGVDALATPLGQV
                     RIDAEGVKIASALPQVLRSSEAHAREHSLEVQLPFLQRVLGDDFTLVPLVVGHASAAQ
                     VAGVLDALWGDDDTLIVISTDLSHFLSYGEAQALDAATARQILDLDESLEPEQACGAY
                     PVRGFLQAARHHGLAPRMLDLRNSGDTSGDRRRVVGYASFAFSQEAA"
     gene            622..1197
                     /gene="amrA"
                     /locus_tag="VGB85_23860"
     CDS             622..1197
                     /gene="amrA"
                     /locus_tag="VGB85_23860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HEY0137149.1"
                     /translation="MTAAHKTQTDPRGEILLGLARAAIEAALGGPPLPPGKPEPWLDA
                     PGACFVTLTQDGQLRGCIGNILPQGSLRDAIIRNAQSSALRDPRFRPLTRDELDHTRI
                     EISLLSAMESIPADSEAALLAALRPGTDGLLLTARGRSAVFIPSVWEQLPDKREFLDH
                     LRAKGHFPRDRWLPDTQAERFTAEHWEEPPR"
     gene            1194..2279
                     /gene="amrS"
                     /locus_tag="VGB85_23865"
     CDS             1194..2279
                     /gene="amrS"
                     /locus_tag="VGB85_23865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEY0137150.1"
                     /translation="MTTPETQHPGRWYHPMPDGRIQCDLCPRECTLQDGQRGFCFVRQ
                     RAGDQIVLTTYGRSSGFCVDPIEKKPLSHFYPGTSVLSFGTAGCNLGCRFCQNHDISK
                     ARDADTLADAAGPEEIAAAAERLGCASVAFTYNDPVIFAEYAMDIADACRERGINSVA
                     VTAGYMSAEPRREFYAKMDAANIDLKAFTEQFYKKVAFAELAPVLETLEYVKKETKTW
                     LEITTLLIPGHNDSDDELHKLSQWVFEKLGPEVPLHFSGFHPDFKMQDVPATPPSTLT
                     RARAIARGNGLQNVYTGNVHDPAGASTHCPGCQTPVIRRDWYQILDYRVTAEGTCPDC
                     HTAIPGRFRAEGQGNWGRKRLRVWMKD"
     gene            2316..4622
                     /locus_tag="VGB85_23870"
     CDS             2316..4622
                     /locus_tag="VGB85_23870"
                     /inference="COORDINATES: protein motif:HMM:NF042814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEY0137151.1"
                     /translation="MPGAIDRLASRPRVARGDAAAALRALLDGLDGAAIDPTLLHQAL
                     VALLLRAVFLRLAGDPLFAAPPGWRAFSVACARVSLPTPLFDPQRHPLLTTLRIDDRA
                     LARALTAITALPSLGAAHEALLALRLEPDPTGRLEVRPSDHRRRSGSHYTPDALAAPL
                     VATTLRPLLGEDPTPAQILDLRICDPALGCGVFLRVACEQLTAILLRAWTRHGAPDVP
                     DLPTLARGKLARECLYGVDRDPLAVELARLSLWLHVADPTLPLASFESTLRHGDSLVG
                     TTHRDAPLDEASADAHVAAVFAGKRGLTRTHAILPLHWPLAFPRVFTRPRPGFDAMLG
                     NPPYFWGNRIGRAFGEDYRRWLKHLHPDAHGNSDLAAHFLRRAFDLLRDGGGLGFITT
                     NSISEAETRKAGLDHVAARGGVIHHATRDLAWPGDASVRVAIVCVHRGPVDGPHHLDG
                     RPVPAIASDLRPPGAGAPPRLPARAAQSFKGVDFGGAGFLLSASARDALLQAAPHEAP
                     YIWPVLNASGLTRSPGLAPTGHIINLGGLTLDEAAAHAPHCLEHLRHHVLPRRMQDRR
                     SGRRDRWWNYNEACPGLYRAIAGLPRVLVGPVVATHLVFAFVAPRVVFTNALNIFTFS
                     TPCTLALLQSRIHALWAAHHGSSLRTDLRYNPTTCFETFPFPPPDPALALAGQIYDEH
                     RAALMSSHHEGLTKIYHRFHDPAERAPDIDQLRHLHDAIDRAVLGAFGWTDLDPRCEF
                     LPLPGTDRLRLRWPQPLADEVLARLASI"
BASE COUNT          674 a         2025 c         1371 g          700 t
ORIGIN      
        1 cgcctacaag cagctgatgg cgatggaccc gaagccgcgg cgcgtcgtgc tgctcgggcc
       61 ggcccacacc gtcgcgttcc gcggcctcgc cctgcccggc gtcgacgcgc tcgccacccc
      121 gctcggccag gtccgcatcg acgccgaggg cgtcaagatc gccagcgccc tgccgcaggt
      181 gctccgctcg agcgaggccc acgcgcgcga gcactcgctc gaggtccagc tgccgttcct
      241 gcagcgcgtg ctcggcgacg acttcaccct cgtgccgctc gtcgtcggcc acgccagcgc
      301 cgcccaggtc gcgggcgtgc tcgacgccct gtggggcgac gacgacacgc tgatcgtcat
      361 cagcaccgac ctctcgcact tcctgtccta cggcgaggcc caggccctcg acgccgcgac
      421 cgctcgccag atcctcgacc tcgacgagtc cctcgagccc gagcaagcct gcggcgcgta
      481 ccccgtccgc ggcttcctgc aggccgcccg ccaccatggc ctcgccccgc gcatgctcga
      541 cctgcgcaac tccggcgaca cctccggcga ccgccgccgc gtcgtcggct acgcatcctt
      601 cgcattctcc caggaggccg catgaccgcc gcacacaaga cccagaccga cccgcgtggt
      661 gagatcctgc tcggcctcgc gcgcgccgcg atcgaggccg cgctcggcgg tccgccgctg
      721 ccgcccggca agcccgagcc ctggctcgac gcccccggcg cctgcttcgt cacgctgacg
      781 caggacggcc agctgcgcgg ctgcatcggc aacatcttgc cgcagggcag cctgcgcgac
      841 gcgatcatcc gcaacgcgca gtcctccgcg ctccgcgacc cccgctttcg cccgctgacc
      901 cgcgacgagc tcgaccacac gcggatcgag atctcgctgc tctcggcgat ggagtcgatc
      961 cccgccgaca gcgaagccgc attgctcgcg gccctgcgcc ccggcaccga cggcctgctg
     1021 ctgaccgcac gtggtcgcag cgccgtgttc atcccctccg tctgggagca gctccccgac
     1081 aaacgcgaat tcctcgatca cctgcgcgcc aagggtcact ttccgcgtga ccgctggctg
     1141 cccgacaccc aggccgagcg cttcaccgcc gagcactggg aggaaccacc tcgatgacta
     1201 ctcctgagac ccaacatccg ggccgctggt atcacccgat gcccgacggc cgcatccagt
     1261 gcgacctttg cccgcgcgag tgcacgttac aagacggcca gcgcgggttc tgcttcgtgc
     1321 gccagcgcgc cggcgaccag atcgtgctga ccacctacgg ccgctcgtcc ggcttctgcg
     1381 tcgacccgat cgagaagaag ccgctgagcc acttttatcc cggcaccagc gtcctgtcct
     1441 tcgggacagc cggctgcaac ctcggctgtc gcttctgcca gaaccacgac atctccaagg
     1501 cccgcgacgc cgacaccctc gcagacgccg ccggccccga ggagatcgca gccgccgccg
     1561 agcgcctcgg ctgcgccagc gtcgccttca cctacaacga ccccgtcatc ttcgccgagt
     1621 acgcgatgga catcgccgac gcctgccgcg agcgcgggat caacagcgtc gccgtcaccg
     1681 ccggctacat gagcgccgag ccgcgccgcg agttctacgc caagatggac gccgccaaca
     1741 tcgacctcaa ggccttcacc gagcaatttt acaagaaggt cgcgttcgcc gagctcgcgc
     1801 cggtgctcga gaccctcgag tacgtcaaaa aagagaccaa gacctggctc gagatcacga
     1861 ccctcctgat ccccggccac aacgacagcg acgacgagct ccacaagctc tcgcagtggg
     1921 tctttgaaaa gctcggaccc gaggtcccgc tgcacttctc gggcttccac cccgacttca
     1981 agatgcagga cgtcccggcg accccgccgt cgacgctgac ccgtgcccgc gcgatcgccc
     2041 gcggcaacgg cctgcagaac gtctataccg gcaacgtcca cgaccccgcc ggcgcgtcca
     2101 cccattgccc cggctgtcag actccggtca tccggcgcga ctggtatcag atcctcgact
     2161 atcgcgtcac cgccgagggc acctgccccg actgtcacac cgcgatcccc ggccgcttcc
     2221 gggccgaggg ccagggcaac tggggtcgta agcgcctgcg cgtgtggatg aaggactgac
     2281 gggcccgcgc gccgggtgct aacgtcccgt cacgcatgcc cggcgcgatc gatcggctcg
     2341 cctcgcgtcc ccgggtcgcg cgcggcgacg cggccgcggc cctgcgcgcg ctgctcgacg
     2401 gcctcgacgg cgccgcgatc gatccgaccc tgcttcatca agccctcgtc gcgctgctgc
     2461 tgcgcgccgt gttcctgcgc ctcgcaggcg accccctgtt cgccgcaccg ccgggctggc
     2521 gcgcgttctc ggtcgcctgc gcgcgcgtct cgctcccgac acccctcttc gatccgcagc
     2581 gccatccgct gctcaccacg ctgcggatcg acgaccgcgc cctcgcacgc gccctgaccg
     2641 cgatcaccgc cctgccctcg ctcggcgcgg cccacgaggc cctgctcgcc ctgcgcctcg
     2701 agcccgaccc gaccggccgt ctcgaggttc gccccagcga tcatcgccgc cgcagcggct
     2761 ctcactacac ccccgacgcg ctcgctgccc cgctcgtcgc cacgaccctg cggccactgc
     2821 tcggcgagga tccgaccccc gcacagatcc tcgacctgcg gatctgcgat ccagcgctcg
     2881 gctgcggcgt cttcctgcgc gtcgcctgcg agcagctcac cgcgatcctg ctgcgcgcgt
     2941 ggacccgcca cggcgccccc gatgtccccg acctcccgac cctcgcgcgc ggcaagctcg
     3001 cccgcgagtg cctgtacggc gtcgaccgcg atccactcgc cgtcgagctc gcgcgcctgt
     3061 cattgtggct acacgtcgcc gacccgaccc tgccgctcgc gagcttcgag tcgacgctgc
     3121 gccacggcga cagcctcgtc ggcaccaccc accgtgatgc tcccctcgac gaagcgagcg
     3181 ccgacgccca cgtcgccgcc gttttcgccg gcaagcgcgg gctaacgcgc acgcacgcga
     3241 tcctcccgct gcactggccg ctcgcctttc cccgggtctt cacgcgcccg cgccccggct
     3301 tcgacgcgat gctcggcaac ccgccctact tttggggcaa ccggatcggc cgcgctttcg
     3361 gcgaggacta tcgccgctgg ctcaagcacc tgcacccgga cgcgcacggc aacagcgacc
     3421 tcgccgccca cttcctgcgc cgcgccttcg atctgctgcg tgacggcggc ggcctcggct
     3481 tcatcaccac caattcgatc agcgaggccg agacccgcaa ggccggcctc gatcacgtcg
     3541 ccgcccgcgg cggcgtaatt catcacgcca cccgcgacct cgcgtggccc ggcgacgcca
     3601 gcgtgcgcgt ggcgatcgtc tgcgtgcatc gtggccccgt cgacggtcct caccacctcg
     3661 acggccgtcc agtcccggcg atcgccagcg acctgcgccc acccggcgcc ggcgctcccc
     3721 cgcgcctccc cgcccgcgcc gcccagagct tcaaaggtgt cgacttcggc ggcgccggct
     3781 tcctgctgtc cgccagcgcc cgcgacgccc tgttgcaggc cgccccgcac gaggccccgt
     3841 acatctggcc cgtgctcaac gccagcggcc tgacccgcag cccgggcctc gcccccaccg
     3901 gccacatcat caacctcggc ggcctcaccc tcgacgaggc cgccgcgcac gccccccact
     3961 gcctcgagca tcttcgccat cacgtgttgc cgaggcgcat gcaggaccgc cgcagcggcc
     4021 gccgcgatcg ctggtggaac tataatgagg cctgcccggg cctctatcgg gcgatcgccg
     4081 gcctcccgcg cgtgctcgtc ggccccgtcg tcgccaccca cctcgtgttc gccttcgtcg
     4141 ccccgcgcgt cgtcttcaca aacgccctca acatcttcac cttctccacc ccgtgcacgc
     4201 tcgccctcct gcagtctcgc atccacgccc tctgggccgc ccaccacggc tccagcctgc
     4261 gcaccgacct ccgctacaac cctaccacct gttttgaaac gttccccttc ccgccccccg
     4321 accccgcgct cgccctcgcc ggacaaattt atgatgagca ccgcgccgcg ctgatgtcgt
     4381 cgcatcacga gggattgaca aagatctacc accgcttcca cgaccccgcc gagcgtgccc
     4441 cggacatcga ccagctccga catttgcatg atgcgatcga ccgcgccgtg ctaggcgcgt
     4501 tcggctggac cgacctcgac cctcgctgcg agttcctccc gctgcccggc accgaccgcc
     4561 tgcgcctgcg ctggccgcag ccgctcgccg acgaggtcct cgcccgcctc gcctccattt
     4621 aattaaatta aaaaaataat cacagtccgc aggccgcgat catacgccgc cgttcgccct
     4681 catcgggcac ccgaccgcgc cccgccgcca tgttgtcggc catgtgcgcc ggcttgcgcg
     4741 tcgccggcaa cggacagtgc accgcaggat
//
LOCUS       DASWNJ010001432         2335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_273993, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001432 DASWNJ010000000
VERSION     DASWNJ010001432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273993"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1566
                     /locus_tag="VGB85_23875"
     CDS             <1..1566
                     /locus_tag="VGB85_23875"
                     /inference="COORDINATES: protein motif:HMM:NF039585.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HEY0137152.1"
                     /translation="NLPADFIAEGLDQTRGWFYTLHVLSSALFDRPAFKNVIVNGLIL
                     AADGKKMSKRLKNYPDPAKVIDSYGADALRAYLINSAVVRGEPMKFGKNAQDLTGECV
                     KETVRVAILPLWNAFNFLVTYARADGWTPSAADLESQPLENPLDRWISSRVGGFLAEL
                     THEYETYELSNLVPAFLRACDDLNNWYIRRGRRRYWRAKAGNDADKNQAYATLYRVLV
                     TLSRAMAPVLPFLTEFLHQRLVVDTGLAREGEDSVHLCPFPRTDSFARDEELETEVEL
                     ARAIVALGLTLRERERIGARRPLGTLTVVSHAPAIVERLKRRDSSDILGELNVKQIEV
                     SGDDSALVDLAAKPNLKLLGKRLGNKLKAVGAALQTVDQQALRAFAAGGTLTIEGEVL
                     QGEDVLLVRAPKPGLVVASEGALTVALDTTMTGELKLEGLAREIINRVQNLRKTADLD
                     VSQRISVTLHCAGDLAAAARQPELADLIRGETLASALAVVENVDAAGPHVLEDSIDGE
                     PLKISLVPTTEAT"
     gene            1563..>2335
                     /locus_tag="VGB85_23880"
     CDS             1563..>2335
                     /locus_tag="VGB85_23880"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEY0137153.1"
                     /translation="MNSPKISTKPPSGMRDFLPDELARRHHVIEVVRRVYGSHGFVPL
                     ETPTIENLSTLLGKYGEEGDQLLYRVLRRRDDLGRALDEVHRLMSGDTAAADMRAQLQ
                     APLADEGLRYDLTVPLARVVAQYELPKYFKRYQIQPVWRADRPGKGRFREFYQCDVDV
                     TGTQSLIAEAEVCGAVTEVLHALGFTDFKIRLNHRQLLRSMMITAGIDLEHEAIALTA
                     VDKLDKIGQGGVREELAGRGISREAADKLMEMILFAQVDS"
BASE COUNT          391 a          781 c          834 g          329 t
ORIGIN      
        1 aacctgccgg cggacttcat cgccgagggc ctcgatcaga cccgcggctg gttttataca
       61 ttgcatgtgc tgtcgagcgc gctgttcgac cggccggcct tcaaaaatgt gatcgtcaac
      121 gggctgatcc tcgccgccga cggcaagaag atgtcgaagc ggctcaagaa ttatcccgac
      181 ccggcgaagg tgatcgacag ctacggggcg gacgcgctgc gggcctacct gatcaacagc
      241 gcggtggtcc gcggcgagcc gatgaagttc ggcaagaacg cccaggacct gaccggcgag
      301 tgcgtgaagg agaccgtccg cgtggcgatc cttccgctgt ggaacgcgtt caacttcctc
      361 gtcacctacg cgcgcgcgga cggctggacg ccgagcgcgg cggacctcga gagccagccg
      421 ctggagaacc cgctcgaccg ctggatctcg tcgcgggtcg gtgggttcct ggccgagctc
      481 acccacgagt acgagaccta cgagctgtcg aacctggtgc ccgcgttcct gcgcgcctgc
      541 gacgatctta ataattggta tatccgccgc ggacgtcgcc gctactggcg cgccaaggcg
      601 ggcaatgacg ccgacaagaa ccaggcctac gcgaccctct accgcgtgct ggtgacgctg
      661 tcgcgggcga tggcgccggt gctgccgttc ttgaccgagt tcctgcacca gcgcctggtg
      721 gtcgacaccg ggctcgcgcg cgagggcgag gacagcgtgc acctgtgccc gttcccgcgg
      781 acggacagtt ttgccaggga tgaggagctg gagaccgagg tcgagctggc gcgggcgatc
      841 gtcgcgctgg gcttgacgct gcgcgagcgc gagcgcatcg gcgcccgccg gccgctcggg
      901 acgctgacgg tcgtcagcca cgcgccggcg atcgtcgagc gcctgaagcg gcgcgacagc
      961 agcgacatcc tcggcgagct caacgtcaag cagatcgaag tgtcgggcga cgactcggcg
     1021 ctcgtcgacc tcgccgccaa gccgaacctc aagctgctcg gcaagcggct cggcaacaag
     1081 ctcaaggcgg tcggcgcggc gctgcagacg gtcgaccaac aggccctgcg ggcgttcgcc
     1141 gcgggtggca cgctgacgat cgagggcgag gtgttgcagg gtgaggacgt gctgctggtc
     1201 cgcgcgccca agcccggcct ggtggtcgcc agcgaggggg cgctgacggt cgcgctcgac
     1261 acgacgatga ccggggagct caagctcgag gggctggcgc gggagatcat caaccgcgtg
     1321 cagaacctgc gcaagacggc cgacctcgac gtcagccagc ggatctcggt cacgctgcac
     1381 tgcgcgggcg acctcgcggc ggcggcccgc cagcccgagc tcgccgacct gatccgcggc
     1441 gagacgctgg cgagcgcgct cgcggtggtc gaaaatgtcg acgcggcggg gccacatgtc
     1501 ctcgaggaca gcatcgacgg cgagcccctg aagatcagcc tggtcccgac gacggaggcg
     1561 acatgaacag cccgaagatc tccaccaagc cgcccagcgg catgcgcgac ttcctgccgg
     1621 acgagctggc ccggcggcat cacgtgatcg aggtggtccg ccgggtctac ggcagccacg
     1681 gcttcgtgcc gctcgagacg ccgacgatcg agaacctctc gacgctgctc ggcaagtacg
     1741 gcgaggaggg cgaccagctg ctgtaccgcg tgctacgccg gcgcgacgac ctcgggcgcg
     1801 cgctcgacga ggtgcatcgc ctgatgagcg gcgacacggc cgcggcggac atgcgcgcgc
     1861 agctgcaggc gccgctcgcc gacgagggtc tgcgctacga cctgacggtg ccgctggccc
     1921 gcgtggtcgc gcagtacgag ctgccgaagt acttcaagcg ctaccagatc cagccggtct
     1981 ggcgcgccga tcgcccgggc aagggccgct ttcgcgagtt ctaccagtgc gacgtcgacg
     2041 tcacgggcac gcagagcctg atcgccgagg cggaggtgtg cggggcggtg accgaggtgc
     2101 tgcacgcgct cggcttcacc gacttcaaga tccgcctcaa ccaccgccag ctgctgcgca
     2161 gcatgatgat cacggcgggc atcgacctcg agcacgaggc gatcgccctg acggcggtcg
     2221 acaagctcga caagatcggc cagggcggcg tgcgcgagga gctcgcgggc cgcgggatct
     2281 cgcgcgaggc ggcggacaag ttgatggaga tgatcctgtt cgcgcaggtc gactc
//
LOCUS       DASWNJ010001433         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_26223, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001433 DASWNJ010000000
VERSION     DASWNJ010001433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26223"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1126)
                     /locus_tag="VGB85_23885"
     CDS             complement(32..1126)
                     /locus_tag="VGB85_23885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137154.1"
                     /translation="MPGRFHFRSIPELDDGWRDPRPGPRLEDLRARGEAFARAFKADC
                     PARGVRTVDLLPFPYPVAFGFWQAAPTRAKYLLMRNRFNLIEFEDFSGHGRTLLVNPT
                     EADLSARAGFFKEVRRELAPFVPPELLRYADGPAKLLRDKLGVDPARVDYITFDHLHV
                     QDLRRGLVGEGGRPPLYSNAKLLVNRRELETLQNIHPLQAPWWISGALDGVPEEKIVV
                     LEGSTWLGKGVALVWTPGHTLGNHSIVFNAPGHGVHAVSENAISPECYLPAASKIRAL
                     RDHARKYSVEVILNGNALESALSQYTSMVLEKSIVDRGAAGGEVPNILCSSPFIRSPL
                     AWGLGPSFVTPPINAGVLGHSLDAPAGEAA"
     gene            complement(1144..>2351)
                     /locus_tag="VGB85_23890"
     CDS             complement(1144..>2351)
                     /locus_tag="VGB85_23890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0137155.1"
                     /translation="EALSFAADPEVRGLSGRGPVTPDHVIRTKQRPLVLDAVPERGIE
                     GAVDAALADYRREYVEYFKGQCAAKRVERKMLDPDPRVVLVPGLGIVAAGADEAAAKV
                     AADLYEHTIAVIRDAEAVGRYQALPDGDIFDMEYWSLEQAKLGKATPTPLAGQVVYVT
                     GAASGIGLACARRFAAAGAHLYLVDRDGERLEAAAKALKAGFEVVDVTRRAEVEASID
                     RVVLRFGGVDGVISNAGAAFQAPIAECPEDQLRASLELNLLAHQWVAAAAMRVLARQG
                     SGGFLLFNASKAAFNPGEGFGPYAVAKAGLVALMKQYALEGARIGVRANAVNADRVRT
                     GLFPPEVVEARAAARGVSPAAYFSANLLGREVTAQDVAEAFLGLALAASTTGAVLPVD
                     GGNLAASPR"
BASE COUNT          363 a          898 c          751 g          339 t
ORIGIN      
        1 agccgactct gccagctcgg gcgagcgacc ttcatgctgc ctcccccgcg ggcgcgtcga
       61 ggctgtggcc gagcacgccc gcgttgatcg gcggcgtcac gaagctcggc ccgaggcccc
      121 aggccagcgg cgagcggatg aacggcgacg agcacaggat gttcgggacc tccccgccag
      181 cggcgccgcg atcaacgatg ctcttctcga gcaccatcga ggtatattgt gacagcgcgc
      241 tctcgagcgc gttgccgttg aggatgacct ccaccgagta cttgcgggcg tggtcccgca
      301 gcgcgcggat cttcgaggcc gccggcaggt agcactccgg cgagatcgcg ttctccgaca
      361 ccgcgtgcac gccgtggcca ggcgcgttga acacgatcga atggttgccg agcgtgtgcc
      421 ctggcgtcca caccagcgcg acgcccttgc cgagccacgt cgacccctcg agcacgacga
      481 tcttctcctc cggcaccccg tcgagcgccc cggagatcca ccacggcgcc tgcaaggggt
      541 ggatgttttg cagagtctcg agctcgcggc ggttgaccag cagcttcgcg ttcgaataaa
      601 gcggcggccg gccgccctca ccgacgagcc cgcgccgcag gtcctgcacg tgcaagtgat
      661 cgaaggtgat gtaatcgacg cgcgccgggt cgaccccgag cttgtctctt aataattttg
      721 ccgggccgtc ggcatatctc agcagctcgg gtggcacgaa cggcgcgagc tcccgtcgca
      781 cctctttaaa aaatcctgcc cttgcggaca ggtcggcctc ggtcgggttg accagcagcg
      841 tgcgcccgtg ccccgagaag tcctcgaact cgatcaggtt gaagcggttg cgcatcaaca
      901 ggtacttggc gcgcgtcggc gcggcctgcc agaagccgaa cgccaccggg tatgggaacg
      961 gcagcaggtc gaccgtgcgg acgccccgcg ccggacaatc ggccttgaac gcgcgcgcga
     1021 aggcctcgcc gcgggcccgc agatcctcca gcctggggcc gggtcgtggg tctcgccagc
     1081 cgtcgtcgag ctcggggatc gacctgaagt ggaacctgcc cggcatgacc gttactttta
     1141 ccctcagcgc ggcgacgctg ccaggttgcc gccgtcgacc ggcaacaccg cgcccgtcgt
     1201 gctcgccgcc agcgccaggc cgaggaacgc ctccgcgacg tcctgcgcgg tcacctcgcg
     1261 gccgagcagg ttggcgctga agtacgccgc cggcgacaca ccccgcgcgg ccgcccgcgc
     1321 ctcgaccacc tccggcggga acagccccgt gcgcacccgg tccgcgttga ccgcgttggc
     1381 gcgcacgccg atacgcgcgc cctccagcgc gtactgcttc atcaacgcga ccagcccggc
     1441 cttggcgacc gcgtacggcc cgaagccctc acccgggttg aacgccgcct tcgacgcgtt
     1501 gaacagcagg aacccgccgc tgccctggcg cgcgagcacc cgcatcgccg ccgcggcgac
     1561 ccactgatgg gccaacaaat tgagctcgag gctggcgcgc agctggtcct cgggacactc
     1621 ggcgatcggc gcctgaaatg ccgcccccgc gttcgagatc acgccgtcga caccgccgaa
     1681 gcgcagcacc acgcgatcga tgctcgcctc gacctcggcg cgcctggtga cgtcgacgac
     1741 ctcgaacccg gccttcagtg ccttcgcagc cgcctccagg cgctcaccgt cgcggtcgac
     1801 caggtacagg tgggcccccg ccgccgcgaa gcgccgcgcg caggccaggc cgatgccgga
     1861 cgccgccccg gtgacgtaca cgacctgccc ggcgagcggc gtcggcgtcg ccttgccgag
     1921 cttggcctgc tcgagcgacc agtattccat gtcaaaaatg tcgccgtcgg gcagcgcctg
     1981 atagcgcccg accgcctccg cgtcgcggat caccgcgatc gtgtgctcat acagatccgc
     2041 cgcgaccttc gccgcggcct cgtccgcccc cgccgcgacg atcccgagcc ccggcaccag
     2101 caccacccgc gggtccgggt cgagcatctt gcgctcgacc ctcttcgccg cgcactgccc
     2161 tttaaaatac tcgacatact cgcggcgata atctgccagc gccgcatcga cggcgccctc
     2221 gatcccacgc tccggcacgg cgtcgagcac gagcggccgc tgcttggtgc ggatcacatg
     2281 atcgggcgtg accggcccgc gcccgctcaa cccgcgcacc tccggatctg ccgcaaagga
     2341 cagggcctcc g
//
LOCUS       DASWNJ010001434         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_407887, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001434 DASWNJ010000000
VERSION     DASWNJ010001434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407887"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="VGB85_23895"
     CDS             <1..1165
                     /locus_tag="VGB85_23895"
                     /inference="COORDINATES: protein motif:HMM:NF013314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0137156.1"
                     /translation="PCRLHADSIRWDLTSEQPGDDPPRPFSFRTAAITQAQISCWTCH
                     TTPEAHAIIRANLHRAPMYNGRIASIGPRYCPSIEDKVVRFADKEQHHLFLEPEGRTT
                     KEIYVNGLSTSLPLDVQDAILAAIPALAGAHVLRHGYAVEYDVVAPDQMDHRLECRRV
                     AGLHFAGQINGTSGYEEAAAQGLIAGASAALWALGRPPLTIARDQGYIGVLVDDLVTQ
                     GCDEPYRMFTARAEYRLLLREDNAEARLNDLAFATGLIDAERHARVQTRVAKITAMAA
                     AIARRKDPGEAPAWLRERAEAQACYADYLVRQQREVDRMRGDASDLPIDPATDYHRLA
                     GLTTEAAERLARVRPTSTGQVARIPGITPAALMCVWAHARAALREAAEAAAAT"
     gene            1211..>2062
                     /locus_tag="VGB85_23900"
     CDS             1211..>2062
                     /locus_tag="VGB85_23900"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY0137157.1"
                     /translation="MSHPADLAADTAVERIGPGRFATTLPRHWDYLLPSGGVLMTVGL
                     RAIAAELAELAELGDAELRPLSATTIFCSPVPAGAIEVGVTVLRRGSVATQARASMFV
                     GGELCLEIVATFGRDRPLPEASLHSAPVVADPDRLPVIDSADQSFTPPFIKNFDHRRA
                     LGSAWWRPEQWVAGDRSGYWYRYLRPQRLADGRLDPLCLPPLIDTMPPALCMMLGPDA
                     LAFLAPSLDLTVHFLADTTSEWLLTHVHCRHARGGWATADIEIWDESRRLLAYGTQMM
                     ILRRRSAL"
BASE COUNT          280 a          788 c          710 g          284 t
ORIGIN      
        1 cccttgccgg ctgcacgccg attcgatccg ctgggacctg accagcgagc aacccggcga
       61 cgatccgccg cgcccgttca gcttccgcac cgcagccatc acccaggcgc agatcagctg
      121 ctggacctgt cacacgaccc ctgaggcgca cgcgatcatc cgcgccaacc tccatcgcgc
      181 gccgatgtac aacgggcgca ttgcttcgat cggtccgcgc tactgcccct cgatcgagga
      241 caaggtggtg cgcttcgccg acaaggagca gcaccacctc ttccttgagc cggaaggccg
      301 cacgaccaag gagatctacg tcaacggcct gtcgaccagc ctgccgctcg acgtgcagga
      361 cgcgatcctc gccgcgatcc ccgccctcgc cggcgcgcac gtgctgcgcc atggctacgc
      421 ggtcgagtac gacgtggtgg cgcccgacca gatggatcat cgcctggagt gccgccgcgt
      481 cgccggcctg cacttcgccg gccagatcaa cggcacctcc ggctacgagg aggcggcggc
      541 gcagggcctg atcgccgggg ccagcgcggc gctgtgggcc ctcgggcgcc cgccgctgac
      601 gatcgcccgc gatcagggct acatcggcgt gctcgtcgac gacctcgtca cccagggctg
      661 cgacgagccg taccgcatgt tcaccgcgcg cgccgagtat cgcctgctgc tgcgtgagga
      721 caacgccgag gcgcgcctca acgacctggc cttcgcgaca ggtttgatag atgccgagcg
      781 ccacgcgcgg gtgcagacgc gggtcgcgaa gatcaccgcg atggcggccg cgatcgcgcg
      841 gcgcaaggac cccggcgagg cgccggcgtg gctgcgcgag cgcgccgagg cgcaggcgtg
      901 ctacgccgac tacctcgtgc gccagcagcg agaggtcgac cgtatgcgcg gcgacgcctc
      961 ggacctcccg atcgaccccg cgaccgacta ccaccgcctc gccggcctga cgaccgaggc
     1021 ggccgagcga ctcgcccgcg tgcgcccgac gagcaccggc caggtcgcgc ggatccccgg
     1081 catcacgccg gcggcgctga tgtgcgtgtg ggcgcacgcg cgcgcggccc tgcgcgaggc
     1141 ggccgaggcg gccgctgcga cctgaccctt gtgacctgtg cgttacgacc ttgcccggga
     1201 gacatgtccg atgagccacc ctgccgacct cgccgccgac accgccgtgg agcggatcgg
     1261 gcccgggcgc ttcgccacga ccttgccgcg ccactgggac tacctgctgc cctcgggcgg
     1321 ggtgttgatg accgtcgggc tgcgggcgat cgccgccgag ctggccgagc tggccgagct
     1381 cggtgacgcc gagctgcggc cgctgtcggc gacgacgatc ttctgctcgc cggtgccggc
     1441 gggcgcgatc gaggtcggcg tgacggtcct gcgccgcggg tcggtcgcca cccaggcgcg
     1501 cgcctcgatg ttcgtcggcg gtgagctgtg cctcgagatc gtggcgacct tcggccgcga
     1561 ccgcccgctg cccgaggcga gcctgcacag cgcgccggtc gtcgccgacc ccgaccgcct
     1621 gccggtgatc gacagcgccg accagagctt cacgccgccg tttatcaaga acttcgacca
     1681 ccggcgcgcg ctcggctcgg cgtggtggcg gccggagcag tgggtcgccg gcgaccgcag
     1741 cggctactgg taccgctacc tgcgaccgca gcggctcgcc gacggtcgcc tcgatccgct
     1801 gtgcctgccg ccgttgatcg acaccatgcc gccggccctg tgcatgatgc tcggcccgga
     1861 cgcgctcgcc ttcctcgcgc cgagcctcga cctgacggtc cacttcctcg ccgacaccac
     1921 gagcgagtgg ctgctgaccc acgtgcattg tcggcacgcc cgcggcggct gggcgaccgc
     1981 ggacatcgag atctgggacg agtcgcgccg gctgctcgcg tacggcacgc agatgatgat
     2041 cctccgccgc cggtcggcgc tg
//
LOCUS       DASWNJ010001435         1860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_130072, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001435 DASWNJ010000000
VERSION     DASWNJ010001435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130072"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VGB85_23905"
     CDS             complement(<1..334)
                     /locus_tag="VGB85_23905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0137158.1"
                     /translation="MSAGRESRARRLRGRLLCPRPAEARVESIADGLLEIDAAGMIAA
                     VGPAPPGCDEPESYPGAVILPGFVDTHLHFPQTRILGSASGPLLPWLERSVFPEEARF
                     AEARYAAAV"
     gene            complement(331..>1860)
                     /locus_tag="VGB85_23910"
     CDS             complement(331..>1860)
                     /locus_tag="VGB85_23910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137159.1"
                     /translation="KKGYRVEEGTLTIEGGVTLAFEPGAEMTVGYQKPGKLFIKGTPD
                     KPVLFTSTGSKEIGAWRGLYLYDHADGSEISGLHLEFGGEGIRGPVYVLADDVKIDDS
                     VIRDILDVPVHVSQTGRLVSFKGNRMERVSTTTMMLLPPSSMPAVGADNVFPEGSVIH
                     VLTGVVRERVRWANPGVPLIIGGLIEIAGEDDDTEALLELTPGTTLHFDEDAYINVGY
                     YRPGSLRAMGTAEAPIVFTSYERKQPGAWRGVNLYKNATASFSHATFEYGGRRVDSGV
                     LYANSRAALAVSDCKFLHNDGGVVLEGGELRISEFVRNTFEASIPALHASAQAFGAIG
                     EGNVFPKEAQLRIEGGRVERDARWRDPGQPIEVTSPISVQGEATLTIDAGVDLRVRDG
                     FTLAIGEVYGGSVQINGTAEAPVTIFGVHDRRGTWDAIRMHPRSSKNVISHLRLRNAG
                     GEAAVSVLGAVDLKVDHLECARCYSPALTWTCASQVAASEVTFADGSPAGLSAPTGCG
                     T"
BASE COUNT          263 a          711 c          614 g          272 t
ORIGIN      
        1 cgaccgcggc ggcgtagcgc gcctcggcga agcgcgcctc ctccgggaac accgagcgct
       61 cgagccacgg cagcagcggt ccgctcgcgc tgccgaggat ccgcgtctgc gggaagtgca
      121 ggtgcgtgtc gacgaagccc ggcaggatca ccgcgccggg atagctctcg ggctcgtcgc
      181 agccgggcgg cgccggaccg accgccgcga tcatgcccgc ggcgtcgatc tcgagcaggc
      241 cgtccgcgat cgactcgacg cgcgcctcgg ccgggcgcgg gcacaacaga cgcccgcgca
      301 gccgcctcgc ccgcgactct cgacccgcgc tcacgtcccg cacccggtcg gcgccgacag
      361 ccccgcgggg ctgccgtcgg cgaaggtcac ctcggacgcg gccacctgcg aggcacatgt
      421 ccaggtcagc gccgggctgt agcagcgcgc gcactcgagg tggtcgacct tgaggtcgac
      481 cgcgccgagc accgacaccg ccgcctcgcc gccggcgttg cgcaggcgca ggtgcgagat
      541 cacgttcttg ctcgaccgcg ggtgcatgcg gatcgcgtcc caggtgccgc ggcggtcgtg
      601 gaccccgaag atcgtcaccg gcgcctcggc ggtgccgttg atctgcacgc tgccgccgta
      661 tacctcgccg atcgccagcg tgaagccgtc gcgcacgcgc aggtcgacgc cggcgtcgat
      721 cgtcaacgtc gcctcgccct gcaccgagat cgggctcgtc acctcgatcg gctggcccgg
      781 gtcgcgccag cgggcgtccc gctcgacgcg cccgccttcg atccgcagct gggcctcctt
      841 cgggaacacg ttgccctcgc cgatcgcccc gaacgcctgc gccgaggcgt gcagcgccgg
      901 gatcgacgcc tcgaaggtgt tgcgcacgaa ctccgagatc cgcagctcgc cgccctctag
      961 caccacgccg ccgtcgttgt ggaggaactt gcagtcgctg accgccagcg ccgcgcggct
     1021 gttggcgtac aacaccccgg agtcgacccg gcgaccgccg tactcgaagg tcgcgtggga
     1081 gaagctcgcc gtcgcgtttt tatagagatt gaccccgcgc cacgcgcccg gctgcttgcg
     1141 ctcgtacgag gtgaacacga tcggcgcctc ggcggtgccc atcgcccgca gcgagcccgg
     1201 gcggtagtag ccgacgttga tgtacgcgtc ctcgtcgaag tgcagcgtcg tgcccggcgt
     1261 cagctcgagc aaggcctcgg tgtcgtcgtc ctcgcccgcg atctcgatca gcccgccgat
     1321 gatcaacggc acgcccgggt tggcccagcg cacgcgctcg cgcacgacgc cggtcaacac
     1381 gtggatcacc gagccctcgg ggaacacgtt gtcggccccg accgccggca tcgagctcgg
     1441 cggcagcagc atcatcgtcg tcgtgctgac gcgctccatg cggttgccct tgaagctgac
     1501 cagccgcccc gtctgcgaca cgtgcaccgg cacatcgagg atgtcgcgga tgaccgagtc
     1561 gtcgatcttt acgtcgtcgg cgagcacgta caccggcccg cggatgccct cgccgccgaa
     1621 ctcgaggtgc aggcccgaga tctccgagcc gtccgcgtgg tcgtagagat acaacccgcg
     1681 ccacgcaccg atctccttcg accccgtcga ggtgaacagc accggcttgt ccggcgtgcc
     1741 cttgatgaac agcttgcccg gcttctgata gccgaccgtc atctcggcgc cgggctcgaa
     1801 ggccagcgtc acgccgccct cgatcgtcag cgtgccctcc tcgacccggt aacccttctt
//
LOCUS       DASWNJ010001436         1914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_622327, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001436 DASWNJ010000000
VERSION     DASWNJ010001436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622327"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1523
                     /locus_tag="VGB85_23915"
     CDS             <1..1523
                     /locus_tag="VGB85_23915"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEY0137160.1"
                     /translation="TGARAMAGAWYRYLRDERRLEPQRIALLRDAEVTPRRISRAIER
                     ARRQARHGTLWVVVIGHISSAQAGEYGELWLADGDGVSHSQAIAPLLGRAGYGKHNQA
                     VVILDGCMSGGSALSGTWTPAPPRYRARLAMPGRDARNDRERLGRRRREPSDVAIFSA
                     GLGPGCVEQLPGARFPALSYLLLQGLRGWADRDGNGNVKAVELLAHTALLLRAALPAE
                     PRPQPSLYGADITLARRVAAAGPTLAQLRPPGAPVVAEATLLSEPLAYIADPMVGFER
                     GGFVMGCPDGDRDCERDEKPAYQVRLSRFQIDPLEVTQAEYRGCVEAGVCAPVEPRRC
                     FVWTGEAFKRGAPLPEPLTRDDHPVVCVSWFQAAMYCEALGKHLPTEAEWERAAAGTT
                     RRRYPWGAAEPRCELAHFDGCGEHTRPVGLRPAGATPEGVRDLAGNVSEWIHDWYDRE
                     AYRRPWRNNPSGPTYGIVRVVRGGSYYDGPTTLRASYRYGLNPVSGFSTVGFRCAR"
     gene            1596..>1914
                     /locus_tag="VGB85_23920"
     CDS             1596..>1914
                     /locus_tag="VGB85_23920"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY0137161.1"
                     /translation="MRRSAALLAATVLWACGSVKNPPVPPPSSAELAGGGVWVAYNLG
                     CKAGCDQISRGDRILAVDGRPVATGAELDAANLTRGTPVKVQVARHDGSGPVEVSLLA
                     TPHT"
BASE COUNT          231 a          669 c          761 g          253 t
ORIGIN      
        1 gtacgggcgc gcgggcgatg gcgggcgcgt ggtaccgcta tttgcgcgac gagcggcgcc
       61 tcgagccgca gcgaatcgcg ctgctgcgcg acgcggaggt cactccgcga aggatctccc
      121 gcgcgatcga gcgggcgcgc cggcaggccc gtcacggcac gctgtgggtc gtcgtcatcg
      181 ggcacatctc gagcgcgcag gcgggcgagt acggcgagct gtggctcgcg gacggagacg
      241 gcgtgagcca ctcgcaggcg atcgcgccgc tgctcggacg cgccggctac ggcaagcaca
      301 accaggcggt ggtgatcctc gatggttgta tgtcgggcgg gtcggcgctg tcggggacct
      361 ggacgccggc gccgccgcgc taccgggcgc gcctcgccat gccggggcgg gacgctcgca
      421 atgaccgcga gcggctgggc cggcgccggc gcgagccgtc ggatgtggcg atcttcagcg
      481 cggggctcgg gccgggctgc gtcgagcagc tgccgggcgc ccgcttcccg gcgctgtcgt
      541 acctgctgct gcaggggctg cgcggctggg ccgaccgcga cggcaacggc aacgtcaagg
      601 cggtcgagct gctcgcccac acggcgctgc tgctgcgcgc cgcgctgccc gcggagccgc
      661 gcccgcagcc gagcctgtac ggcgccgaca tcacgctcgc gcggcgagtc gcggccgcgg
      721 gcccgacgct ggcgcagctg cggccgccag gggctccggt ggtcgcggag gcgacgctgc
      781 tgagcgagcc gctggcgtac atcgccgacc cgatggtcgg cttcgagcgc ggcggcttcg
      841 tgatgggttg tcccgacggc gaccgcgact gcgagcgcga cgagaagccg gcgtatcagg
      901 tcaggctctc gcgcttccag atcgacccgc tcgaggtgac gcaggcggag tatcgcgggt
      961 gcgtcgaggc gggggtgtgc gcgccggtcg agccgcggcg ctgcttcgtg tggaccggcg
     1021 aggcgttcaa gcgcggggcg ccgctgcccg agccgctgac gcgcgacgac cacccggtgg
     1081 tgtgcgtgag ctggtttcag gccgcgatgt attgcgaggc gctcggcaag cacctgccga
     1141 ccgaggccga gtgggagcgg gcggcggccg gcacgacgcg gcgcagatat ccctggggcg
     1201 cggccgagcc gcgctgcgag ctggcccact tcgacggatg cggcgagcac acgcgcccgg
     1261 tcggcctgcg gccggcgggc gccaccccgg agggggtgcg agacctcgcc ggcaacgtgt
     1321 cggagtggat ccacgactgg tacgaccgcg aggcgtaccg gcggccgtgg cgtaacaacc
     1381 cgtccgggcc cacgtacggg atcgtgcgcg tggtccgggg cgggagttac tacgacgggc
     1441 cgaccacgct gcgcgcgtcg tatcgctacg ggctcaaccc ggtgtcgggc tttagcaccg
     1501 tcggctttcg ctgcgcgcgc tgagatggcg ggggccgacg gcgttcaggt gcgggatccc
     1561 tcgacgcgtg cttcgcgggg agtatgctcg gccgcatgcg aagatccgcc gccttgctgg
     1621 ctgccacggt gctctgggcc tgcgggtccg tgaagaatcc tcccgtccca ccgccgtcct
     1681 cggccgagct cgcgggcggc ggcgtgtggg tcgcctacaa cctcgggtgt aaggccggct
     1741 gcgaccagat ctcgcgcggg gaccggatcc tcgcggtcga cggccgcccg gtcgccaccg
     1801 gcgccgagct cgacgccgcc aacctgaccc gcggcacgcc ggtcaaggtg caggtcgccc
     1861 gccacgacgg cagcgggccg gtcgaggtct cgctgctcgc cacgccgcac accg
//
LOCUS       DASWNJ010001437         2865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_377051, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001437 DASWNJ010000000
VERSION     DASWNJ010001437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377051"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="VGB85_23925"
     CDS             <1..1118
                     /locus_tag="VGB85_23925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137162.1"
                     /translation="ARAHGVPNTRLPRRPALRYVDLRRGGRLSHPFTVAVAGRVSAVR
                     RILELARYLVTMGRFRRRDPPGFTGSIEDPTATGKIEDWFSARSLRFPFDALFPIAGN
                     ALTGGHAPSVPAIYYLKLLALLQRFSLPTLLGLRFPHFAAGNDTLWRRIAASHDTRHG
                     EAVRRLVRGACVTVETSRAVHEFDRVIWTAPLDRFAEIADASDDERDLFGRVRHTCRS
                     VVTCRVAGLPQRPIMYQAIGIPDGPEVLPYSWYEVRAGSGIYNFYSHLRYPDEHTPRA
                     TLGEVVRGIESMLARFGATLVEVHRARVWDKFFPHFGGDDLRAGIHARLDAMQGRGGL
                     YFAGELLAHVSVPLNVEYAVDLVHRHFGQRMLPAAAP"
     gene            complement(1196..2434)
                     /locus_tag="VGB85_23930"
     CDS             complement(1196..2434)
                     /locus_tag="VGB85_23930"
                     /inference="COORDINATES: protein motif:HMM:NF040195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137163.1"
                     /translation="MAQRVEQALQYAPAPKLEARVTPRSPWEAQGPTSTPRDGSDAQA
                     ETPAAAPAATTTPGLPGLPTSPRDPAVRAAFTQGGTQGRAQAPSGVRSFTIPDLYCPF
                     DPAVHPQAAVVHAGTIAWAKAMGLARDEKHVQALHKAKVGWLVARAFPIADKMPALQI
                     AADWITLFCLIDNHIESIRGPALSHVYLKSLLDVFREGAAPKIIDPYAQAFADLRLRM
                     IDVDVPFWITRFGEQLARLFGTFVDEAKYRQLEAVPEAAKYRKMREVSSGLYFGFRLG
                     ELTDNINLPPHVRDDPTVRNLESKASLIVGLANDIYTVEKELAKGEVNNMVMVLMHEE
                     GLTIDEALTRTVELHDAETHEFIALTRRLPKFSREIDADLQRYVEVLSSMISGHRSWA
                     LETSRYTPGTADAHGRIEDR"
BASE COUNT          380 a          967 c         1053 g          465 t
ORIGIN      
        1 acgcgcgggc ccacggcgtg cccaacactc gcctgccgcg gcggcccgcg ctgcgctacg
       61 tcgatctgcg gcgcggcggt cggctctcgc atcccttcac ggtcgccgtc gcgggacgcg
      121 tctcggcggt gcgacggatc ctcgagctcg cgcgctatct ggtgacgatg gggcggtttc
      181 gccggcgcga cccgccgggc ttcaccggat cgatcgagga cccgaccgcg accgggaaga
      241 tcgaggactg gttctcggcc cgctcgctgc gcttcccctt cgacgcgctg ttcccgatcg
      301 ccggcaacgc gttgaccggc ggccacgcgc cgagcgtgcc ggcgatctac tacctcaagc
      361 tgctcgcgct gctgcagcgc ttctcgctgc cgacgctgct cggcctgcgc ttcccgcact
      421 tcgcggccgg caacgacacg ctgtggcggc gcatcgcggc gagccacgac acgcgccacg
      481 gcgaggcggt gcgccggctc gtccgcggcg cgtgcgtgac ggtcgagacc tcgcgcgcgg
      541 tccacgagtt cgatcgtgtg atctggaccg cgccgctcga tcgcttcgcg gagatcgccg
      601 acgcgtcgga cgacgagcgc gatctattcg gacgtgtacg ccacacctgc cggtcggtgg
      661 tgacgtgtcg ggtcgcgggc ctgccccagc ggccgatcat gtaccaggcg atcgggatcc
      721 ccgacggtcc ggaggtgctg ccgtactcgt ggtacgaggt gcgcgcgggc tcggggatct
      781 acaacttcta ctcgcacctg cgctacccgg acgagcacac gccgcgcgcg accctcggcg
      841 aggtcgtgcg cggcatcgag tcgatgctcg cccgcttcgg cgcgacgctc gtcgaggtcc
      901 accgcgcgcg cgtgtgggac aagttcttcc cgcacttcgg cggcgacgac ctgcgcgcgg
      961 ggatccacgc caggctcgac gcgatgcagg gccggggcgg gctctacttc gccggcgagc
     1021 tgctcgcgca cgtgtcggtg ccgctcaacg tcgagtacgc ggtcgacctg gtgcaccgcc
     1081 acttcggcca gcgcatgctg ccggccgccg cgccatgatc atgcgggcca tgcgggccat
     1141 gcgggccatg cgggccatgc ggcgggcccc atggtcagcg gtcagcggtc agcggtcagc
     1201 ggtcctcgat gcggccgtgg gcgtcggcgg tgccgggggt gtagcgggag gtctcgagcg
     1261 cccagctgcg gtggccggag atcatggagc tcaggacctc gacgtagcgc tggaggtcgg
     1321 cgtcgatctc gcgggagaac ttcggcaggc gtcgggtgag ggcgatgaac tcgtgggtct
     1381 cggcgtcgtg gagctcgacg gtgcgggtca gcgcctcgtc gatcgtcagg ccctcctcgt
     1441 gcatcaacac catgaccatg ttgttgacct cgcccttggc gagctccttc tcgacggtgt
     1501 agatgtcgtt ggcgaggccg acgatcagcg aggccttgct ctcgaggttg cgcacggtcg
     1561 ggtcgtcgcg cacgtgcggc ggcaggttga tgttgtcggt cagctcgccg aggcgaaagc
     1621 cgaagtacag gccgctgctg acctcacgca tcttgcgata cttggcggcc tccgggacgg
     1681 cctcgagctg gcggtacttg gcctcgtcga cgaaggtgcc gaacaggcgc gcgagctgct
     1741 cgccgaagcg ggtgatccag aacgggacgt cgacgtcgat catgcgcagg cgcaggtcgg
     1801 cgaacgcctg ggcgtagggg tcgatgatct tcggcgcggc gccctcgcgg aacacgtcga
     1861 gcaggctctt gaggtacacg tgcgagagcg ccgggccgcg gatgctctcg atgtggttgt
     1921 cgatcaggca aaacagcgtg atccagtcgg cggcgatctg gagcgcgggc atcttgtcgg
     1981 cgatcgggaa ggcgcgcgcg accagccagc cgaccttggc cttgtgcagc gcctgcacgt
     2041 gcttctcgtc gcgggcgagg cccatcgcct tggcccacgc gatcgtgccc gcgtgcacga
     2101 ccgccgcctg cgggtgcacg gcggggtcga agggacagta gaggtcgggg atcgtgaagc
     2161 tgcgcacgcc gctcggcgcc tgtgcgcggc cctgcgtgcc gccctgcgtg aacgcggcac
     2221 gcaccgccgg gtcgcggggg ctggtcggca ggcccggcag acccggggtc gtggtggccg
     2281 cgggagcagc agctggggtc tcggcctgcg cgtcggaccc gtcccggggg gtggacgtcg
     2341 ggccctgggc ctcccacggc gaacgcggtg tcacccgggc ctcgagtttc ggggcaggtg
     2401 catactggag ggcttgttcg acgcgctggg ccatggggtc actccgggga gacagtacat
     2461 gcgtggacag tcttgactat gccccggatc gacggcaacg cctaccgcga ggcgagtgac
     2521 attcgacgag gcgcacagcc tgtctcgtac atgtatgtgc gtcgatcgag ggccgcgggg
     2581 atcacggttc gcgcgtgaca tgacactgcc tgtacctcgc accttggcat gtcgatatgc
     2641 ccacatgccg acatcaccac gcccgcagct ccaccggtcc gcatgtcgat gtgtccgcct
     2701 ggccacacgt ccgcatggac acatcgcgcg atgctccgag gtccggggac gcacggccgg
     2761 atgctcgcgt gagggtcccg accgcggtcc gcgggcggtt cgcaggtact tctcctccgg
     2821 ggtacgcatg gagcacgcgc accactggac cgctcggagg ggtcg
//
LOCUS       DASWNJ010001438         3649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_274044, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001438 DASWNJ010000000
VERSION     DASWNJ010001438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274044"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="VGB85_23935"
     CDS             complement(<1..1017)
                     /locus_tag="VGB85_23935"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEY0137164.1"
                     /translation="MRLAQPLPWERRVAVWDAAFAVVRAHLRGAGMREVSTPVRVAAP
                     AAEPYIDPIAAPPGWLATSPELAMKRLLCRGSGPVFQLSHVFRRAERGDRHSEEFHLL
                     EWYRLGGSLAAVEGDVEAIVAGVYEAAAQVLEIDAAPPRRWIRASFFEVFAATTGVAL
                     RGDEDEEELRAIVTPELRAQVWDERPGPLLSPDRTVRTLAAWTAVFSAWSDAALDPWL
                     SGQVGAGVHLGEFPAGLAALAESGAQGGRVVAHRVECHVAGVELANGYRELRDALEQR
                     RRFAAVHALRAALDQPQLATDEDFLAELGGLPVCAGMALGLDRLIMLACGRTRLGDVS
                     LALGA"
     gene            complement(1025..1585)
                     /gene="efp"
                     /locus_tag="VGB85_23940"
     CDS             complement(1025..1585)
                     /gene="efp"
                     /locus_tag="VGB85_23940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY0137165.1"
                     /translation="MDVSELRKNAKLQMDGHPYVVTDFQFVKPGKGQGLYKCRLKNMI
                     TGATIDRTWRSGERLEAANVESRRMQYLFAGGGSFTFMDSENYEQVELSGDLVGDDKN
                     FLYEQLEVEVLFYNNRPVGVTLPSHIIMKIVESEPGVKGDSANNPTKLATLETGYKLQ
                     VPLFVKEGERVKIDTRTGDYIERVNS"
     gene            1943..2356
                     /locus_tag="VGB85_23945"
     CDS             1943..2356
                     /locus_tag="VGB85_23945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY0137166.1"
                     /translation="MQLTTKARYGLRALCHVAHRGYASAHVIAEQQAIPSRYLEEIIG
                     ELRKAGFVVGKRGPRGGYKLARPAADIAVAEVLQALSTPANGGPSTGDPQDEITRMVE
                     RSVDARFEAAVGQLSLEQLLGEARETHRRRTDLVV"
     gene            2373..3338
                     /gene="cysK"
                     /locus_tag="VGB85_23950"
     CDS             2373..3338
                     /gene="cysK"
                     /locus_tag="VGB85_23950"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEY0137167.1"
                     /translation="MQRAALVDDILGLIGNTPLCRIRTLADPATHATVWGKCEMLNPG
                     GSVKDRIALAMVEAAEAAGQITPGVSVLVEPTSGNTGIGLALVAAIKGYRLILTMSES
                     MSLERRNLLRAYGAEIVLTQADRGIEGAVEKARELCQRPAHFMLDQFGNPQNPLVHRR
                     TTGPELLAQMQGLTIDGFVAAVGTGGTITGVSGVLRAAHPDVMIVAVEPDRSAVLSGE
                     SPGPHKIQGMGAGFIPPVLDRDAYARIARITDEAAYRMKQRLAQEEGLLVGISAGAAM
                     VAAIDLARELGPGKHVITVLCDTGERYFSLDEYFVGSADLRTPGP"
     gene            3340..>3649
                     /locus_tag="VGB85_23955"
     CDS             3340..>3649
                     /locus_tag="VGB85_23955"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEY0137168.1"
                     /translation="MAGRYLVLGAGGLGCPALLGLLGADPAEITIVDHDVVDASNLHR
                     QVLYSVAEVGMAKVEAAALRLRGRAPRLRVHPLRLRLTADNLGALLDAQRQDTVVLEC
                     S"
BASE COUNT          546 a         1283 c         1271 g          549 t
ORIGIN      
        1 ggcgcccagc gccagcgaga cgtctccgag gcgcgtgcga ccgcaggcga gcatgatcag
       61 tcgatcgagg cccagcgcca tgccggcgca gaccggtagg cccccgagct cggccaggaa
      121 gtcctcgtcg gtcgccagct gcggctggtc gagcgcggcc cgcagcgcgt gcacggcggc
      181 gaagcgacgc cgctgctcca gcgcgtcacg cagctcgcga tagccgttgg cgagctcgac
      241 gccggcgacg tggcactcga cgcggtgggc gacgacacga ccgccctgcg cgccgctctc
      301 ggcgagcgcg gcgaggcccg cggggaactc gccgaggtgc acgccggcgc cgacctgtcc
      361 cgagagccag ggatcgagcg ccgcgtccga ccacgcgctg aacacggccg tccacgcggc
      421 gagcgtgcgc acggtgcggt cggggctcag caagggtccc ggtcgttcgt cccacacctg
      481 cgcgcgcagc tcgggagtga cgatcgcccg cagctcctcc tcgtcctcgt cgccgcgcag
      541 cgcgacgccg gtggtcgccg cgaagacctc gaagaagctg gcccggatcc atcgccgcgg
      601 cggcgcggcg tcaatctcga ggacctgcgc ggcggcttca tacacgccgg cgacgatcgc
      661 ctcgacgtcg ccctcgaccg cggcgagcga accaccgagg cggtaccact cgagcaggtg
      721 aaactcctcg ctgtgccggt cgccgcgctc ggcccggcga aacacgtgcg agagctggaa
      781 caccgggccg ctgccgcgac acagcaggcg cttcatcgcc agctcggggc tggtcgccag
      841 ccagcccggc ggcgccgcga tcggatcgat gtagggctcg gccgccgggg ccgcgacgcg
      901 caccggcgtg ctgacctccc gcatgcccgc accacgcagg tgcgcgcgca cgacggcgaa
      961 cgccgcgtcc catacggcga cgcggcgttc ccagggcagc ggttgcgcga ggcgcatgcg
     1021 cgctctagct gttgacgcgc tcgatgtagt cgccggtgcg ggtgtcgatc ttgacgcgct
     1081 cgccttcctt gacgaacagc gggacctgca gcttgtagcc ggtctcgagc gtggcgagct
     1141 tggtcgggtt gttggccgag tcgcccttca cgccgggctc ggactcgacg atcttcatga
     1201 tgatgtggct cggcagggtc acgccgaccg ggcggttgtt gtagaacagg acctcgacct
     1261 cgagctgctc gtacaggaag ttcttgtcgt cgccgacgag atccccgctg agctcgacct
     1321 gctcgtagtt ctcgctgtcc atgaaggtga agctgccgcc gccggcgaac agatactgca
     1381 tgcgccgcga ctcgacgttg gccgcctcga gccgctcacc cgagcgccag gtgcggtcga
     1441 tcgtcgcgcc ggtgatcatg ttcttgagcc ggcacttgta gagcccctgg cccttgcccg
     1501 gctttacgaa ttgaaagtcg gtgaccacgt aggggtgacc gtccatctgg agcttggcgt
     1561 tcttgcggag ttcggagacg tccatgggga tcgcagtgtg cctattccct ccgccccccc
     1621 gctgtcaacg cccgccctcc ggcgcgcgcc cgcgcgaagt ctcgcgaacc ccccggcgag
     1681 ggagatcgcg cctccgaccc gcctcactcc agcgccccgc gcgctgccgc gcggatttcc
     1741 ccattgtgac gcgcggagcg ggcctgcagg cctggcgtgc ctttttcgat ttgtctccag
     1801 gttttatttg cacatattta cacggcgaaa atggcccaaa aacagcgctc aacgatgaaa
     1861 aatgcggcgc aaaaccgatg actcgggccg gattcttgtg gcaatctgcc gccgcggctt
     1921 ttcatcaagg tcgacgggaa aaatgcaact tacgaccaag gcacgatacg gattgcgcgc
     1981 tctttgccat gtggctcacc ggggatatgc ctccgcccat gtcatcgcag agcagcaggc
     2041 gattccctcg cgatacctgg aggaaatcat tggtgagctg cgcaaggccg gctttgtggt
     2101 cggcaaacgc ggaccccgcg gtggttacaa actcgcgcgt ccggcagcgg acatcgccgt
     2161 ggccgaggtg ctgcaggcgc tgtcgacccc ggccaacggc ggcccctcca cgggcgatcc
     2221 gcaggacgag atcacgcgga tggtcgagcg atcggtcgac gctcgcttcg aggcggcggt
     2281 cggtcagctg agcctcgagc agctgctcgg cgaggcccgc gagacccatc gccgtcgcac
     2341 ggacctcgtg gtgtgagtcg ggtggcccga tcatgcagcg cgcggcgctc gtcgacgaca
     2401 tcctcgggct gatcggcaac acgcccctgt gccggatccg tacgctggcc gacccggcca
     2461 cgcacgcgac ggtgtggggc aagtgcgaga tgctcaatcc gggcggctcg gtcaaggacc
     2521 gcatcgccct ggcgatggtc gaggcggccg aggccgccgg gcagatcacg ccgggcgtca
     2581 gcgtgctcgt cgagccgacc agcggcaaca cgggcatcgg cctcgcgctc gtcgcggcga
     2641 tcaagggcta ccgcctgatc ctgacgatgt ccgagagcat gagcctcgag cggcgcaacc
     2701 tgctccgggc ctatggcgcg gagatcgtgc tgacgcaggc cgaccgcggg atcgagggcg
     2761 ccgtcgagaa ggcgcgcgag ctgtgccagc ggcccgcaca cttcatgctc gatcagttcg
     2821 gtaacccgca aaacccgctg gtccaccggc gcacgaccgg ccccgagctg ctcgcgcaga
     2881 tgcaaggcct gacgatcgac ggcttcgtcg ccgcggtcgg caccggcggc acgatcaccg
     2941 gcgtgtccgg ggtgctgcgc gctgcgcatc ccgacgtcat gatcgtcgcg gtcgagcccg
     3001 atcgcagcgc ggtgctcagc ggtgagtcgc cgggcccgca caagatccag ggcatgggcg
     3061 cgggcttcat cccgccggtc ctcgaccgcg acgcctacgc ccgcatcgcc cgcatcaccg
     3121 acgaggccgc gtaccgcatg aagcagcgcc tggcccagga ggaagggctg ctggtcggga
     3181 tctcggcagg cgcggcgatg gtggcggcga tcgatctggc gcgcgagctc gggcccggca
     3241 agcacgtaat cacggtcctg tgcgacaccg gcgagcgcta cttctcgctc gacgagtact
     3301 tcgtcggctc ggccgacctg cgcacgccgg ggccctgaga tggcgggccg ctacctcgtg
     3361 ctcggcgcgg gtgggctcgg gtgtccggcg ctgctcgggc tgctcggcgc cgatccggcg
     3421 gagatcacga tcgtcgatca cgacgtcgtc gacgccagca acctgcatcg ccaggtgctc
     3481 tacagcgtcg ccgaggtcgg catggccaag gtcgaggcgg cggcgctgcg cctgcgcggg
     3541 cgggctccgc ggctgcgtgt gcacccgctg cgcctgcggc tgaccgccga caacctcggc
     3601 gcgctgctcg atgcgcagag gcaggacacg gtcgtgctcg agtgctccg
//
LOCUS       DASWNJ010001439         2108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_695652, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001439 DASWNJ010000000
VERSION     DASWNJ010001439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695652"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="VGB85_23960"
     CDS             complement(<1..993)
                     /locus_tag="VGB85_23960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137169.1"
                     /translation="MSHALLVTPSRTLAVLLSSLALAGGTFGCNKDEQKKAEPTAATA
                     GTAPADAKDPATTVADVTAPISSAVGAVALASGGRIAPLSAEPDILGHFAIANMSRLL
                     VDVKTQLVPPKYAGFLEEAALRSLLSIALEKRGNLAMNFDMAAPFGCALVDPKVEDIK
                     LGCAFGYKGGAKAFVTDLGEAEKAADAAGHVAAYTVEGKSIYVDALGEGVVVSAGAET
                     FNKTQSYLQRNVVARAGEIHGDIEVVVYMSTVVDRYRDQLAPLFEQADSQEPAPSGNP
                     AVDGAVKAWTDYRKRSGKTTVDRIAEFAQFTGFFSVEPAGVMMGGALFPKAGSRV"
     gene            complement(1186..>2108)
                     /locus_tag="VGB85_23965"
     CDS             complement(1186..>2108)
                     /locus_tag="VGB85_23965"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0137170.1"
                     /translation="PMRPGDPDMHRPRRRHRALLLALLPACAIERYETFLAEHGQTYV
                     TTDAQASSTSEVAETTSSTTAASTSTAASTNGAEAGQDASSEPDTTTTTSSTTTGDTT
                     AGPEPVCGNGVLEPFGWVPEECDDGNLVADDGCNDTCAEDRTIFVSSILYQAGWFESL
                     YLADALCANMAVQVGFSQPLKYRAWLSDSKTHARDRFKVGRGRIVLVNGLVVADSWID
                     LLAGKLQGPVEVTEKGETYHGPVWTGTTPQGTLVPGATNCDDWTSFSSKKAAYYGYSD
                     RATAEWTLSALDDNPISCTVDCAVYCLKER"
BASE COUNT          293 a          745 c          717 g          353 t
ORIGIN      
        1 gacccggctg cccgccttcg ggaacagcgc gccgcccatc atcacgcccg ccggctcgac
       61 gctgaagaag ccggtgaact gcgcgaactc ggcgatgcgg tcgaccgtcg tcttgccgct
      121 gcgcttgcgg tagtcggtcc acgccttgac cgcgccgtcg accgccgggt tgcccgacgg
      181 cgccggctcc tggctgtcgg cctgctcgaa cagcggcgcc agctggtcgc ggtagcggtc
      241 gacgaccgtc gacatgtaca cgacgacctc gatgtccccg tggatctcgc ccgcgcgggc
      301 gaccacgttg cgctgcaggt acgactgcgt cttattaaaa gtctccgcgc cggcgctgac
      361 gaccacgccc tcgccgagcg cgtcgacgta gatcgacttg ccctcgacgg tgtaggccgc
      421 gacgtggccg gcggcgtccg ccgccttctc ggcctcgccg aggtcggtca cgaaggcctt
      481 cgcgccgccc ttgtagccga acgcgcagcc gagcttgatg tcctcgacct tggggtcgac
      541 gagcgcgcag ccgaacggcg ccgccatgtc gaagttcatc gccaggttgc cgcgcttctc
      601 gagcgcgatc gacagcaggc tgcgcagcgc ggcctcctcg aggaagccgg cgtacttggg
      661 cggcaccagc tgcgtcttca cgtcgaccag caggcgcgac atgttggcga tcgcaaagtg
      721 cccgaggatg tcgggctcgg cgctcagcgg cgcgatcctc ccgccgctcg ccagggcgac
      781 cgcgccgacg gccgagctga tcggcgcggt gacatcggcg accgtcgtcg cgggatcctt
      841 ggcgtcggcc ggggccgtgc ctgccgtcgc cgcggtcggc tcggccttct tctgctcatc
      901 cttgttgcag ccgaatgtgc cgccagcgag cgcgagagaa gagaggagca cggcgagcgt
      961 gcgagacggc gtgacgagga gcgcgtgtga catcgtgcgg gcgcgaagaa tacttcaccc
     1021 cccggacgcg cgaaagcccc ccgtagcgct tcgcgatagc gcccccgggg ttacccgggg
     1081 agcagcttcc gggctcgtcg gagccgccgt catcgacgag ccgaccggga gcccggcggt
     1141 cgcgcgagcg gatagccgtg cggacatgtc ccgggttcgc agaggtcagc gctccttgag
     1201 gcagtacacg gcgcaatcga ctgtgcagga gatcgggtta tcgtcgagcg cggacagggt
     1261 ccattcggcg gtggcgcggt cactgtaacc atagtaagcg gcctttttcg atgaaaagga
     1321 cgtccagtcg tcgcagttgg tcgcgcccgg gaccagcgtc ccctgcggcg tggtccccgt
     1381 ccatacgggg ccgtggtacg tctccccctt ctcggtgact tcgacagggc cttgcagctt
     1441 gcccgcgagc aggtcgatcc acgagtcggc gaccaccagc ccgttgacca ggacgatgcg
     1501 cccacgcccc accttgaagc ggtcacgcgc gtgtgtcttg ctgtcgctga gccacgcgcg
     1561 atacttcagc ggctgcgaaa acccgacctg gaccgccatg ttggcgcaca gcgcgtccgc
     1621 gaggtacagg ctctcgaacc agcccgcctg gtagagaatg ctcgacacga agatcgtgcg
     1681 gtcctcggcg caggtgtcgt tgcaaccgtc gtccgcgacg aggttgccgt cgtcgcactc
     1741 ctcgggcacc cacccgaacg gctcgagcac cccgttgccg cacaccggct ccgggcccgc
     1801 cgtggtgtct ccggtcgtcg tcgagctcgt cgtcgtcgtc gtatcgggct cgctgctcgc
     1861 gtcctgaccc gcctcggcgc cgttcgtgct cgccgccgtc gaggtgctcg cggccgtcgt
     1921 gctgctcgtc gtctccgcaa cttccgacgt gctgctggct tgcgcgtccg tcgtcacgta
     1981 cgtctggccg tgctccgcca agaatgtctc ataacgctcg atcgcgcagg ccggcagcag
     2041 ggccagcaac agcgcgcggt gcctgcgtcg gggtcggtgc atatcggggt ctccgggtcg
     2101 catgggtc
//
LOCUS       DASWNJ010001440         1879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_447735, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001440 DASWNJ010000000
VERSION     DASWNJ010001440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447735"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VGB85_23970"
     CDS             <1..312
                     /locus_tag="VGB85_23970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137171.1"
                     /translation="QPPNPASARFAEAGKQIIVTMRNSDSLLTCEDGAEADFRIHAGG
                     DSIVTDCVAQGHELILTVQGDASMATGINYLGHIGPGPWILNENRIGLLSFWKVPIAP
                     E"
     gene            488..1756
                     /locus_tag="VGB85_23975"
     CDS             488..1756
                     /locus_tag="VGB85_23975"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY0137172.1"
                     /translation="MADEHRPEEERRQEHRPPIGVGPREVRLIIPVRTILLLLVSALA
                     VWLLIELWAIALTVVVALILVGTLNPFVAKLQRRGMKRGWAVLWILLVLLVVASLLLL
                     LTLPPLFDQMLELLEDAPAKRDAFIAWLNQRPLTAPLAPLVRDAGSEHMFATVGSALL
                     SVSSQIVMTIGYAATAIVLAFYILLDGQRSQAGLYAIVPRSHHLRLARIMLELEVIVG
                     GYVRGQLITSVAITIFTLALLTAFQVPNALPLAVFAGLTDVIPFIGGALATTPAVIGG
                     LAVSPTVAIIIAAVMLSYQEFESRYLVPKVYGRVLRLSATTVILALMIGGTLLGILGA
                     LLALPISAGLLMIARELRVELPGDDSDDGSLRARDRAAEATYAKLSAGASPEAAVAIA
                     SEMARARRKGEAIEPALAGDTRAGDPHLGG"
BASE COUNT          249 a          725 c          611 g          294 t
ORIGIN      
        1 caaccaccga acccggcgtc ggcccgcttc gccgaagcgg gcaagcagat catcgtcacc
       61 atgcgcaaca gcgattcgct gctcacctgc gaggacgggg ccgaggccga ctttcgcatc
      121 cacgccggcg gcgactcgat cgtcaccgac tgcgtcgcgc agggccacga gctgatcctg
      181 accgtccagg gcgacgcctc gatggcgacc ggcatcaact acctcgggca catcggcccc
      241 ggcccgtgga tcctcaacga gaaccgcatc ggcctgctct cgttctggaa ggtcccgatc
      301 gcgccggagt gacgacgccg ggggtccgca cgcgggccgg gctcggacac gaacctcgcg
      361 tcctgcgcag gcgccgtcac acgcggaccg ccgcagcgcg gccagcgaca tcgatcacga
      421 tcgggccgca tattgcggtc acgagtctcg ccgcaccacg atatccgcgg tacgctcgcc
      481 ctcgcgcgtg gctgacgaac atcgaccgga agaagagcgg cgccaggagc atcgcccccc
      541 catcggcgtc ggcccacgcg aggtgcggct gatcatcccc gtgcgcacga tcttgctgct
      601 gctggtcagc gcgctcgcgg tgtggctgct gatcgagctc tgggcgatcg cgttgaccgt
      661 cgtcgtcgcg ctgatcctcg tcggcacgct caaccccttc gtcgccaagc tgcagcgccg
      721 cggcatgaag cgcggctggg cggtgctgtg gatcttgctc gtgctgctcg tcgtcgcctc
      781 gctgctgctg ctgctgaccc tgccgccgct gttcgaccag atgctcgagc tgctggagga
      841 cgcgccggcc aagcgcgacg ccttcatcgc ctggctcaac cagcgcccgc tgaccgcccc
      901 gctggcgccg ctggtccgcg acgccggctc ggagcacatg ttcgccaccg tcggctcggc
      961 gctgctgtcc gtctcctcgc agatcgtgat gacgatcggc tacgcggcga cggcgatcgt
     1021 gctcgccttc tacatcctgc tcgatggtca acgctcgcag gccggcctct acgcgatcgt
     1081 gccgcgcagc catcacctgc gcctggcgcg gatcatgctc gagctcgagg tcatcgtcgg
     1141 tgggtacgtc cgcggccagc tgatcacctc cgtggcgatc acgatcttca ccctcgccct
     1201 gctgaccgcc ttccaggtgc ccaacgcgct gccgctggcg gtgttcgccg gcctgaccga
     1261 cgtcatcccc ttcatcggcg gcgccctggc gaccacgccc gcggtgatcg gcggcctcgc
     1321 ggtcagcccg acggtggcga tcatcatcgc cgcggtgatg ctgtcgtatc aggagttcga
     1381 gagccgctac ctggtgccca aggtctacgg ccgcgtgctg cgtctgtcgg ccaccaccgt
     1441 catcctcgca ttgatgatcg gcggcaccct gctcgggatc ctcggcgccc tgctcgcgct
     1501 gccgatctcc gccggcctgc tgatgatcgc ccgcgagctg cgcgtcgagc tgccgggcga
     1561 cgactccgac gacggctccc tgcgcgcgcg cgaccgggcc gccgaggcca cctacgccaa
     1621 gctcagcgcc ggcgcctcgc ccgaggccgc cgtggcgatc gcctcggaga tggcccgcgc
     1681 ccgccgcaag ggcgaggcga tcgagcccgc cctcgccggt gatacccgcg ccggcgaccc
     1741 gcacctcggc ggctgaggct gcgaccgccg ctatgccccc gcgaccgagg gcgatcggta
     1801 cgtgccgcca gcgagcaccg tcgcgacggc cgggccgagc tcctcgtcca tcgcctgctt
     1861 gacgacgtag ccgcgcgcg
//
LOCUS       DASWNJ010001441         1841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_237075, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001441 DASWNJ010000000
VERSION     DASWNJ010001441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237075"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1827)
                     /locus_tag="VGB85_23980"
     CDS             complement(<1..1827)
                     /locus_tag="VGB85_23980"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEY0137173.1"
                     /translation="MRSPRYVPATGVLAEPMAFDAAFFGYSASEARTMDPQHRVFLEC
                     AWSALESAGYAPRGFSGAVGVFGGSDVPRYWIERIGATGAALSMEEYAVSFANMSDTL
                     ATRVAYELGLRGPALTVQTACSTSLVAVHLACQSLRAGECDMALAGGVVVLPPDRLGY
                     LSEGGSVVSPDGHCRPFDADAQGMVSSSGAGLVVLRRLADAVADGDTIHAVLHGTAIN
                     NDGAQKVGFTAPSVEGQAGAIARALAMAGFDAATIGYVEAHGTATRLGDPIEVAALTR
                     AYRAHTDLRGYCALGSLKSNLGHLGVAAGVAGLIKAALCLANERIPPTLHFRRPNPEL
                     ELEASPFHVNARTLEWPRGAAPRRAGVSAFGVGGTNAHVVLEEAPAAAAASPSRAAQL
                     LVISARTSGSLDGVTQRLAASFAADGPPLADAAFTLARGRQAFARRRGVVARDGRGAA
                     EALQRGDRTWVAEGVAPGRAPRVAFLFPGGGTQAVGMGRELYAGEAVYRDAFDRAAAG
                     FDAALGIDVRGLLFASDPAIAADALRRPTRNIAAIYCTEYALARLLGSFGVTPSAVTG
                     HSLGEYAAATIAGILTVEDAVRLLVERGQIHEALPADAALLIV"
BASE COUNT          258 a          758 c          608 g          217 t
ORIGIN      
        1 cacgatcaac agcgccgcgt cggcgggcag cgcctcgtgt atctggccgc gctcgaccag
       61 cagacgcacc gcgtcctcga ccgtcaggat ccccgcgatc gtcgccgccg cgtactcgcc
      121 gaggctgtgc cccgtgaccg cgctcggcgt cacgccgaac gatcccagca atctcgccag
      181 cgcgtactcc gtgcaataga tcgccgcgat gttgcgcgtc ggtcggcgca gcgcgtcggc
      241 ggcgatcgcc gggtcgctgg cgaacagcag gccacgcacg tcgatcccca gcgccgcgtc
      301 gaagcccgcc gccgcgcggt cgaacgcgtc gcggtacacc gcctcgcccg cgtacagctc
      361 gcggcccatg cccaccgcct gggtgccgcc ccccggaaac aggaatgcga cccgcggcgc
      421 ccggcccggt gccacaccct cggcgaccca cgtacggtcg ccgcgctgca gcgcctcggc
      481 cgcgccccgg ccgtcacgcg cgaccacccc ccgtcgccgc gcgaacgcct ggcggccacg
      541 tgccagggtg aacgcggcgt ccgccagcgg cggtccgtcg gctgcgaacg acgccgccag
      601 tcgctgcgtc accccgtcga gcgagcccga cgtgcgcgcc gagatcacca gcaattgagc
      661 cgcacgcgac gggctcgcgg cggcggccgc cggcgcctcc tcgagcacca cgtgcgcatt
      721 ggtgccgccg accccgaacg cgctgacccc cgcgcgccgc ggcgccgccc cgcgcggcca
      781 ctccagagtc cgcgcattca cgtggaacgg gctcgcctcg agctcgagct ccgggtttgg
      841 ccgacgaaag tgcagcgtcg gcgggatgcg ctcgttcgcg aggcacagcg ccgccttgat
      901 caaccccgcc acccccgccg ccacgcccag gtgaccgagg ttgctcttga gcgatccgag
      961 cgcgcaatat cctcgcaggt cggtgtgcgc ccggtaggcc cgggtcagcg cagcgacctc
     1021 gatcgggtcg ccgagccgcg tcgcggtccc gtgggcctcg acgtagccga tcgtcgccgc
     1081 gtcgaagcct gccatcgcca gcgcccgcgc gatcgccccg gcctggccct ccacgctcgg
     1141 cgcggtgaaa ccgaccttct gcgcgccgtc gttgttgatc gcggtgccgt gcagcaccgc
     1201 gtggatcgtg tccccgtcgg cgaccgcgtc cgcgagccga cgcagcacca ccaggcccgc
     1261 cccgctgctg ctcaccatgc cctgcgcgtc ggcgtcgaag ggccggcagt gaccgtccgg
     1321 cgacaccacc gaacccccct ccgacaggta tcccagacgg tccggcggca ggacgaccac
     1381 gccgcccgcc agcgccatgt cgcactcgcc cgcgcgcagg ctctggcacg ccagatggac
     1441 cgcgaccagc gaggtcgagc acgcggtctg aaccgtcagc gccggcccgc gcaggccgag
     1501 ctcgtatgcg acccgcgtcg ccagagtgtc ggacatgttg gcgaacgaga ccgcgtactc
     1561 ctccatgctc agcgcagcgc ccgtcgcacc gatgcgctcg atccagtagc gtggcacgtc
     1621 gctgccgccg aacaccccga ccgcgcccga gaacccgcgc ggagcgtacc ccgccgactc
     1681 cagcgccgac caggcgcact cgaggaacac ccggtgctgc gggtccatcg ttcgcgcctc
     1741 gctcgcgctg tagccgaaga acgccgcgtc gaaggccatc ggctcggcga gcacgccggt
     1801 cgccggcaca taccgcggcg agcgcacgag cgcggggtcg a
//
LOCUS       DASWNJ010001442         2879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_518707, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001442 DASWNJ010000000
VERSION     DASWNJ010001442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518707"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(410..1819)
                     /locus_tag="VGB85_23985"
     CDS             complement(410..1819)
                     /locus_tag="VGB85_23985"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0137174.1"
                     /translation="MADDEPETGTILIVEDHQDTRETLRDYLEEFGYVVDTAVDGADG
                     LRKVQARPVDLVITDLNMPHVSGLELLAAIKSHKLTPDVLVLSATGLISKAVEAMRLG
                     AVNYLVKPVDLDQLTSEVRAVMRVRRHREVQRSAAQTTMMLDPGGEPTRPQIAYDLET
                     GQPLPLSGEPVATAEGETPPTIGRYQVLSELGTGGMGTVYKCFDPALRRTVAVKVVAT
                     GTIRRVHRELLLQRFAREAQAAGMLNHPNIVTVYDYHADPRSHSVYLAMEYVAGQSLG
                     AIIAGAGKIPWERATNLAFQIADALEFAHRNQIVHRDVKPENVLVTDGDVAKILDFGI
                     AKLADSELTRPGTFVGSPRYLAPESFRGGGIDYRADQFSLGTVLFEMLTGQAAFKFED
                     FYAGVHRILSEVPPPLASFGVVGPPLLQEIVHRLHQKDPDKRYVDEMELLSDLAELGN
                     LAGLKLQVSIARPSFEARR"
     gene            complement(2021..>2879)
                     /locus_tag="VGB85_23990"
     CDS             complement(2021..>2879)
                     /locus_tag="VGB85_23990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137175.1"
                     /translation="YSKAEQERRHKLPLRLFSGLSRTTAQRLHERLKGAGLKVKVVDD
                     SSITQSRELRKVVVWTVGGLAVPVGVCAALGAPLFVHAVLVGLALFFLAIVFATQRHR
                     RRQRQQPFLKLRTAPALPASDPLVARLAALLTPDTAPELRERVGGLALAVQQLVDHRA
                     AAPGARAEVDALTEPVAPLVALIEAQVQRLRTIDAALVGLDEGDLVRALAASEARGEP
                     PARRDDLLAGLDRLRGLEDERAAIFHRLLEAESLLRRAVALGLGVRDEEAEQSRRVAQ
                     ALHALEAGS"
BASE COUNT          438 a          957 c         1076 g          408 t
ORIGIN      
        1 cgaccgttga tattggtcag gaccgcctcc agcgcgagga ggttgttcgg attatcgtcg
       61 actagcagga tgtctacggg agcttccaaa cagatcctgg gcgcgtgagc gtcggagata
      121 cttgcagcgc cgtctcgcgg cgtcaaaggt tagtcgaggc cgccgtggtg gggaagcacg
      181 cgcggatgct tgccagtgca tcgaagatcg cggccgcggc gccggatgcg ggggcagcgc
      241 tacgccacga cggcgatcgg gggcccggaa ccgcagcgca ggcccggtgg taaagccgga
      301 tcgccgcaag acgagcgccg cgtgagccct cgtgaaggaa ggcagtcgcg caggcggcga
      361 cgcaaacgcc gatttactgc agcgattgcg gcgactgacg gttcgctggt cagcgccgcg
      421 cctcgaacga gggtcgcgcg atactgacct gcagcttgag gccggccaga ttgccgagct
      481 cggcgagatc ggagagcagc tccatctcgt cgacgtagcg cttgtcgggg tccttttggt
      541 gcaagcgatg gacgatctcc tgcaacagcg gaggtccgac gacgccgaag ctcgccagcg
      601 gcggcggcac ctccgagagg atccggtgca cgccggcgta gaagtcctcg aacttgaacg
      661 cggcctgccc ggtcagcatc tcgaagagca cggtgccgag cgagaactgg tcggcgcggt
      721 agtcgatgcc gccgccgcgg aagctctccg gggcgaggta acgcggcgag ccgacgaagg
      781 tgcccggccg cgtcagctcc gagtcggcga gcttggcgat gccaaagtcg aggatcttgg
      841 cgacgtcccc gtcggtgacg agcacgttct cgggcttcac gtcgcggtgg acgatctggt
      901 tgcggtgggc gaactcgagg gcgtcggcga tctggaacgc gaggttggtc gcgcgctccc
      961 acgggatctt gccggcgccg gcgatgatcg ccccgagcga ctgacccgcg acgtactcca
     1021 tcgccaggta caccgagtga ctgcgcgggt cggcgtggta gtcgtagacg gtgacgatgt
     1081 tggggtggtt gagcatgccc gcggcctggg cctcacgggc gaagcgctgc agcagcagct
     1141 cgcggtggac ccggcggatc gtgccggtgg cgacgacctt gaccgccacg gtgcgccgca
     1201 gggccgggtc gaagcacttg tagacggttc ccatgccgcc ggtgccgagc tcgctcagca
     1261 cctgatagcg gccgatcgtc ggcggggtct cgccctcggc ggtcgccacc ggctcgcccg
     1321 acagcggcag cggctggccg gtctcgaggt cgtaggcgat ctgcgggcgc gtcggctcgc
     1381 cgccggggtc gagcatcatc gtcgtctggg cggcgctgcg ctggacctcg cggtgccggc
     1441 ggacccgcat gacggcccgg acctcgctcg tcagctggtc gaggtcgacg ggcttgacga
     1501 ggtagttgac ggcgccgagg cgcatcgcct cgaccgcctt ggagatcagg ccggtggccg
     1561 acagcaccag cacgtcgggg gtcagcttgt gactcttgat cgcggcgagc agctcgaggc
     1621 cgctgacgtg cggcatgttg aggtcggtga tgacgaggtc gacgggacgc gcctgcacct
     1681 tgcgcaaacc atctgcaccg tcgaccgcgg tgtcgacgac atagccgaac tcctcgaggt
     1741 aatcgcgcag cgtctcgcgc gtgtcctggt gatcctcgac gataaggatc gttccagtct
     1801 ctggttcgtc atccgccatc ggggccctcc tgccacacat agcgaacccc ggaggggccg
     1861 acaagggcgc gccgtgatcc cgtgcgagat cactgggagc gcggggacgc ggcggcgcgt
     1921 ggaccccagg cgtggcacga tcggtccgag ttgtcacacc gcgaacgagt gcgcaagcag
     1981 cgctaagggc accgtggcgc attaaagccc gcgcgcgcgc tcagctaccg gcctcgagcg
     2041 cgtgcagggc ctgcgcgacg cgccggctct gctcggcctc ctcgtcgcgg acaccgaggc
     2101 cgagcgcgac ggcgcgacgc agcaggctct cggcctcgag caggcgatga aagatcgcgg
     2161 cgcgctcgtc ctcgaggccg cgcaggcggt cgagcccggc gagcagatcg tctcgccgcg
     2221 cgggcggctc gccgcgggcc tcgctcgccg cgagcgcccg caccagatcg ccctcgtcga
     2281 gcccgacgag cgccgcgtcg atggtccgca ggcgctgcac ctgggcctcg atcaacgcga
     2341 cgagcggcgc gaccggctcg gtcagcgcgt cgacctcggc gcgggccccc ggcgcggcgg
     2401 cgcggtgatc gacgagctgc tggaccgcga gcgcgagccc accgacgcgc tcgcgcagct
     2461 cgggcgcggt gtcgggggtc agcagcgcgg cgaggcgagc gaccagcgga tccgaggcgg
     2521 gcagggccgg cgccgtgcgc agcttgagga acggctgctg gcgctggcgc cggcggtgtc
     2581 gctgggtggc gaagacgatc gcgaggaaga acagcgcgag gccgacgagc accgcgtgga
     2641 cgaacagcgg ggcgccgagc gccgcgcaca cgccgaccgg caccgccagg cctcccaccg
     2701 tccacacgac gaccttacgc agctcgcgcg actgcgtgat cgacgagtcg tcgacgacct
     2761 tgaccttgag gccggcgccc ttgagtcgct cgtgcaggcg ctgcgcggtg gtccgcgata
     2821 gccccgagaa cagccgcagc ggcagcttgt ggcgccgctc ttgctcggcc ttcgagtac
//
LOCUS       DASWNJ010001443         3795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_484789, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001443 DASWNJ010000000
VERSION     DASWNJ010001443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484789"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VGB85_23995"
     CDS             <1..340
                     /locus_tag="VGB85_23995"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEY0137176.1"
                     /translation="IGAAFVTPAGKMKAKHLIHVPNTDKPGGRVQVEDVLRATAAVLV
                     ACRVKGFSSIAVPLMGAFDSGIPAEEAARAIHSELKAYRGDRPLKAYLMAKDADDVEV
                     FELAMEGNIP"
     gene            370..717
                     /locus_tag="VGB85_24000"
     CDS             370..717
                     /locus_tag="VGB85_24000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137177.1"
                     /translation="MALVQIDLRRGPARKHTPGDADAAEGDHAAAHRAAELASAAAAD
                     SADTAAATVPKASLAPAAAGLVLAGGLVLLAPVAVAAAGSVTTRILLIAGLVALGLFA
                     ALLRPALPGVDPQ"
     gene            714..1883
                     /locus_tag="VGB85_24005"
     CDS             714..1883
                     /locus_tag="VGB85_24005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137178.1"
                     /translation="MNGRLRGLALALPVLACIHCSAPDPVLRNEPATLHFTPARPELA
                     VRLVHDGPEPLPLSRIRVDHRDADWAAFTLTDRTLPRQIDPGGEAVLHLRVDVDHFKA
                     AEPHAPRRAGETSLTFNAGEPRRIALRFDDAGTPLAASLIRLGLLALLAAGLTVLGRR
                     ARLPWSIALPALVAVAIAPLGAGLCPTTPTAILGLADLLQCADGRGGVPLQIWPHPDG
                     LGLVIVAFLFAALGRISDDPAALRRSLGLALALVALVLGRSLDPQAAVQAQAGLRWGL
                     WMQPFAAAALAIAAVLEVQAARAHSPVAGRVAAIGLAALVTTLCLGGADLPRQALSLP
                     HAAAVSLGLALWLAEVTALTWLLGRARAPAWARRAVVPLAIAQILLVVLRSWAGP"
     gene            2191..3171
                     /locus_tag="VGB85_24010"
     CDS             2191..3171
                     /locus_tag="VGB85_24010"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEY0137179.1"
                     /translation="MATTIAQIGGFELWDILQLAQTSRMSIKLSVESPHGTGSMFFQG
                     GSLLHAIAGDREGDEAVDIILDWREGSVSSSQLPENCPTTVRHSSIVALLMDRARQRD
                     EQQRDRPSSSSSHSGSPVPAPPAPKFTPPTVASRPLPSQPVDDEQDDTSALDPADEQA
                     SGASDEGTSDDGSHESAEDASDDSSEAPSQVPPDKELSDMDLRAVKVILEEIDDHLMG
                     GLLSVDIISKASGMSLAGINSAPKACALFNRLSEQLHSALTKSEDSLARGINKFIIEG
                     DNGLYIFVIELDERHRWAMLVDGARAQLGMVFVIIPEIQPRLVAAVQARG"
     gene            3177..>3795
                     /locus_tag="VGB85_24015"
     CDS             3177..>3795
                     /locus_tag="VGB85_24015"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY0137180.1"
                     /translation="MVYIHKVYTRFGDQGDTMLASGDTVPKDSLRVASYGEVDELNAT
                     IGLLRIEVSRAQGSAVGDQAAFLADLDLGLARIQQELFDLGAELAEPNAAAFASPGVT
                     VSTGKARLCVTDEDVTRLEQELDALNDPLPSLRSFILPGGGPVGATAHLARTVCRRAE
                     RQAVTLARSEPVRGEAIRYLNRLSDLLFVVARAAAQRLGHDEVLWD"
BASE COUNT          512 a         1507 c         1234 g          542 t
ORIGIN      
        1 catcggggcc gccttcgtca cgcccgcggg gaagatgaag gccaagcacc tcatccacgt
       61 gcccaacacc gacaagcccg gcggcagggt ccaggtcgag gacgtcctgc gagccaccgc
      121 cgccgtgctc gtcgcctgcc gcgtcaaagg cttctcgagc atcgccgtgc ccctgatggg
      181 cgcgttcgac agcgggatcc cggccgagga ggccgcccgc gcgatccaca gcgagctcaa
      241 ggcctaccgc ggcgaccgcc cgctcaaggc ctacctcatg gccaaggacg ccgacgacgt
      301 cgaggtcttc gagctggcca tggaaggcaa cattccctga tccgatgatc aggtgaccag
      361 gagcggaccg tggcgctcgt ccagatcgac ctgcgacgcg gtcccgcgcg caagcacacc
      421 cccggcgacg cggacgcggc cgagggggac cacgcggccg cccaccgggc ggccgagctc
      481 gcgagcgccg ccgcggccga ctccgcggac acggccgcgg caactgtccc gaaggccagc
      541 ctcgccccgg ccgccgccgg cctcgtgctc gccggcggcc tcgtcctgct cgcgcccgtg
      601 gccgttgccg cggccggcag cgtcaccacg cgcatcctcc tgatcgcggg cctcgtcgcc
      661 ctcggcctgt tcgccgccct cctgcgcccc gcgctgcccg gagtcgaccc gcaatgaacg
      721 gccggctccg cggactcgcc ctcgcgctgc cggtgctcgc atgcatccac tgcagcgcgc
      781 cggacccggt cctgcgcaac gagccggcga ccctccactt cacgccggcc cgccccgagc
      841 tcgccgtgcg cctcgtgcac gacggccccg agccgctgcc gctgagccgg atccgcgtcg
      901 accaccgcga cgccgactgg gccgccttca ccctcaccga ccgcaccctg ccgcgacaga
      961 tcgaccctgg cggcgaggcc gtgctgcacc tgcgcgtcga cgtcgatcac ttcaaggccg
     1021 ccgagcccca cgcgccgcgc cgcgccggcg agaccagcct gaccttcaac gccggcgagc
     1081 cgcggcggat cgccctgcgc ttcgacgacg ccggcacacc gctcgccgcc tcgctgatcc
     1141 gcctcggcct gctcgcgctg ctcgccgccg gtctgaccgt gctcggtcgt cgcgctcgcc
     1201 tgccgtggtc gatcgccctg cccgcgctcg tcgccgtggc gatcgccccg ctcggcgccg
     1261 gtctgtgtcc cacgaccccg acggcgatcc tgggcctggc cgatctcctg caatgcgccg
     1321 acggacgcgg cggcgtcccg ctgcagatct ggccccaccc cgacggcctc ggcctcgtga
     1381 tcgtcgcttt cctgttcgcg gcgctcgggc ggatctccga cgatcccgcc gcgctgcgtc
     1441 gatccctcgg cctcgcgctc gccctcgtcg ccctcgtgct cggcaggagc ctcgaccccc
     1501 aggcagccgt gcaggcccag gccggtctgc gctggggctt gtggatgcaa cctttcgcgg
     1561 ccgcggcgct ggcgatcgcc gccgtgctcg aggtccaggc cgcacgggcc cacagccccg
     1621 tcgccgggcg cgtcgcggcg atcggcctcg cggcgctggt gaccacgctg tgcctcggcg
     1681 gggccgatct gccgcggcag gcgctgagct tgccgcacgc ggccgccgtc agcctcggcc
     1741 tcgcgctctg gctcgccgag gtcaccgccc tgacatggct gctgggccgc gcccgggcac
     1801 ccgcgtgggc gcgccgggcc gtcgtaccgc tggcgatcgc ccagatcttg ctcgtggtcc
     1861 tgcgcagctg ggctggcccg tgaccgcgac cggcccgtgc cttcgccact gctggccctc
     1921 cccgggctct gccccgcttc gcgctcaccg cgcgcagcgc ccggtcgtag cttttttcta
     1981 tttggggtgt taggttggcc gaaccaaagc ctaggaccgt aggcttgccc ggcgagcgcg
     2041 gatgctagtt cggaagttca acctcgtatt ccgagtgagg cgggagacaa gcgacggggc
     2101 tcgcaggacg acgcgagcgc gtcggactct cgagagcaca tgcgctgtgg ctccagcaag
     2161 acgggtatcc ccggccgggg gcggtagctg atggcgacga ccatcgctca gatcggtggc
     2221 ttcgagctgt gggacatcct tcagctcgcc cagacctccc gcatgtcgat caagctgtcg
     2281 gtggaatctc cgcacggcac cggttcgatg ttcttccagg gcggcagcct cctgcacgcg
     2341 atcgcgggcg accgcgaggg cgacgaggcc gtcgacatca tcctcgactg gcgcgaaggc
     2401 agcgtgtcct cgtcgcagct gcccgagaac tgcccgacga ccgtgcgtca ctcgtcgatc
     2461 gtcgcgctgc tgatggaccg cgcccgccag cgcgacgagc aacagcgcga ccggccgtca
     2521 tcctcctcga gccacagcgg ctcgccggtc cccgccccgc ccgcgcccaa gttcaccccg
     2581 ccgacggtcg cctcgcgccc gctgccgtcc cagcccgtcg acgacgagca ggacgacacc
     2641 tcggccctcg acccggccga cgagcaagca tccggagctt ccgacgaggg aacttccgac
     2701 gacggctcgc acgagtccgc cgaggatgct tccgacgatt cttccgaggc gccctcgcag
     2761 gtgccgcccg acaaggagct gtccgacatg gatcttcgag ccgtcaaggt gattctggag
     2821 gagatcgacg atcacctcat gggcggtctg ctgagcgtcg acatcatcag caaggcgtcc
     2881 ggcatgtcgc tcgccggcat caactcggcg cccaaggcct gcgccctgtt caaccgcctg
     2941 tcggagcagc tgcacagcgc gctgaccaag tccgaggact cgctcgcgcg tggcatcaac
     3001 aagttcatca tcgagggcga caacgggctc tacatcttcg tgatagagct cgacgagcgg
     3061 caccgctggg cgatgctggt cgacggcgcc cgggcccagc tcggcatggt cttcgtgatc
     3121 atcccggaga tccagcccag gctggtcgcg gccgtgcaag cccgcgggta ggccgaatgg
     3181 tctacatcca caaggtctac acccgcttcg gcgaccaggg cgacaccatg ctcgccagcg
     3241 gcgacacggt ccccaaggac tcgctgcgcg tcgcctccta cggcgaggtc gacgagctca
     3301 acgccacgat cggcctgctg cgcatcgagg tctcgcgcgc gcagggcagc gccgtcggcg
     3361 accaggccgc gttcctcgcc gacctcgacc tcggcctcgc aaggatccag caggagctgt
     3421 tcgacctcgg ggccgagctc gccgaaccaa acgctgctgc attcgcatcc cccggcgtca
     3481 ccgtctcgac cggcaaggcc cggctgtgcg tcaccgacga ggacgtcacg cgcctcgagc
     3541 aggagctcga cgccctcaac gaccccctgc cctcgctgcg cagcttcatc ctgccgggag
     3601 gcggcccggt gggagcgacc gcccacctcg cgcgcaccgt gtgtcgccgc gccgagcgtc
     3661 aggccgtcac cctcgcccgc agcgagcccg tccgcggcga ggcgatccgc tacctcaacc
     3721 gcctcagcga cctgctgttc gtcgtcgccc gcgctgccgc gcagcgcctc ggccacgacg
     3781 aggtcctgtg ggaca
//
LOCUS       DASWNJ010001444         5225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_662342, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001444 DASWNJ010000000
VERSION     DASWNJ010001444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662342"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VGB85_24020"
     CDS             complement(<1..620)
                     /locus_tag="VGB85_24020"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEY0137181.1"
                     /translation="MYKLFKRTYYPGHFEVQIAGACDPGLSREHNEDAIAIQEDDGRG
                     YYMAVVCDGMGGHNAGEVASAMAVEKIRTYLDANFRKQGPRELLYHGFLLASTTISEY
                     SAKNPDTEGMGCTCVMVMGVRDKMWVAHSGDSRAYLVDKKAATAITLDHTMVQELVTK
                     GVITKEQAAVHPYRGRISRCLGHGRSKGVPDINAGIIEHGDNILLC"
     gene            complement(849..921)
                     /locus_tag="VGB85_24025"
     tRNA            complement(849..921)
                     /locus_tag="VGB85_24025"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(886..888),aa:Ala,seq:cgc)
     gene            complement(973..1119)
                     /locus_tag="VGB85_24030"
     CDS             complement(973..1119)
                     /locus_tag="VGB85_24030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137182.1"
                     /translation="MTLALAPTWLAWLTAPKDGPILALLAVVVLALVLGLRSRASAAK
                     DPPA"
     gene            1180..1959
                     /locus_tag="VGB85_24035"
     CDS             1180..1959
                     /locus_tag="VGB85_24035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEY0137183.1"
                     /translation="MARPSRTRLDQLLVERELCESRAIAQALVVAGKVVVGDHTETKP
                     GTLVPADAPVRVRGQVAPFVSRGGQKLRGALDRFAGLEVRGRVVMDIGASTGGFTDCV
                     LQAGADRVYAVDVGYGQMAWKLAQDPRVVVLDRTNIRTLAPERIGEPVTLVVADCSFI
                     SLRKVLPAVPAFLAPQADVVALIKPQFELARGEVGAGGVVRDAGGRAAALAGVLAAAE
                     ALGFQVVDTCDSAIAGQDGNLELLAWLRWDVGSQAVPRDSS"
     gene            2050..2424
                     /locus_tag="VGB85_24040"
     CDS             2050..2424
                     /locus_tag="VGB85_24040"
                     /inference="COORDINATES: protein motif:HMM:NF014087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HEY0137184.1"
                     /translation="MPLPPLSARARAHLKSLAHGLAPLVQVGADGITPGVGQAALVAL
                     EQHELIKVRLGQSYEGERDDAARELVEATGSQLVQVIGRIVVLYRRRSKDDPKRPRIV
                     IPTAKPKAGASKAAEKPGKQAG"
     gene            2491..4329
                     /locus_tag="VGB85_24045"
     CDS             2491..4329
                     /locus_tag="VGB85_24045"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEY0137185.1"
                     /translation="MSATRRALLAASVFAALVGCKPRGTTQPSNPAVWGEPGTGAGSR
                     IEIQRSEAPVGAPSPVLEVLAGEVARNLGQLQSEDREVPAYFLAYDLIARDHLWLEVQ
                     QGQVVRRRLDSDRTIDIDVRVGSPQLDNSHSQGGNYGPGNGLGAGEPVTVQDDPLALA
                     QALWMFTEEQYRDALVALSAAESNEQLRSQDEAKPASPDFSEETAVVHLEPKVQLDLE
                     GLRKAWEPRLKEASVVLGDDTRVLESNVVLLVNVDNRSFVDSNGSKIQSSHVRLRIML
                     SATGQADDGMRLARFESFEVHTPEQLPAQALLVATAKRLREELVALRAAPLAEPYAGP
                     AVLAGRAAGVFFHEIFGHRLEGHRQKDDLEGQTFSTMIGKRVLPVFLDMVDDPTAQSL
                     GGVPLSGHYYVDDEAVPARRTTLVERGKLKGFLLGRSPVLPFKKSNGHGRRERGNQVV
                     ARQGNLIVTSRKTIPDAQLRRTLIEEVQRQGKPYGLWFSDIQGGYTITERSGPQAFKV
                     MPLLVYRVWTDGRPDELVRGVDIVGTPIAAFETIIATGDSPGIFNGMCGAESGDVPVS
                     AVSPSLLLRSLEIERASHDRDKPPLMPPPETLLKNLSRGTGSEVAR"
     gene            4326..>5225
                     /locus_tag="VGB85_24050"
     CDS             4326..>5225
                     /locus_tag="VGB85_24050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137186.1"
                     /translation="MKAALLILLAALADAPANTETTAKPTAKTEPVKPAKAEAPPARS
                     AKAEVPAKPAVREIKPLGKRALDLEVAAIEQELARGVGGLRLPDSPAPYLATVQIIRA
                     SVLSLDGSYGGVITDVVEEQAVAAVEVRVGSPARDQSGTFGNDGARLRFIISLEPSPV
                     LSRHKLWLALDQDFRAATALFAQKQAILARLAGEPPPPDLGPKPEPMPRQPAPDEGSP
                     QLDREGLHTMVMQLSKRFVDHPEIDNGDVFVQVLRTTVTTINSEGLVLHERHDRAVLA
                     VVADTRAADGMHLDAGAAIHLQEL"
BASE COUNT          803 a         1730 c         1952 g          740 t
ORIGIN      
        1 ctgcacagga ggatgttgtc gccgtgctcg atgatgccgg cgttgatgtc tggcacgccc
       61 ttggagcggc cgtggccgag gcagcgtgag atgcgcccgc ggtaggggtg cacggcggcc
      121 tgctccttgg tgatcacgcc cttggtgacg agctcctgga ccatggtgtg gtcgagggtg
      181 atcgcggtgg cggccttctt gtcgaccagg taggcccggc tgtcgcccga gtgcgcgacc
      241 cacatcttgt cgcgcacgcc catgaccatc acgcaggtgc agcccatgcc ctcggtgtcg
      301 gggttcttgg cgctgtactc ggagatcgtg gtgctggcga gcaaaaaccc gtgatagagc
      361 agctcacgcg ggccctgctt gcgaaagttg gcgtcgaggt aggtgcggat cttctcgacg
      421 gccatcgccg aggcgacctc gccagcgttg tgaccgccca tgccgtcgca cacgaccgcc
      481 atgtagtaac cacggccgtc gtcctcctgg atcgcgatgg cgtcctcgtt gtgctcgcgc
      541 gacagacccg ggtcgcacgc acccgcgatc tgcacctcga agtggcccgg gtagtaggtg
      601 cgcttgaaga gcttgtacat gaaccgagcc gggcgccgca gtcagcgggc tgcgatcctg
      661 acacttggcc cgggatacgg caagactgga aacaaccggc cgggagcgcg cgcgccggac
      721 ttcacgcgcc gggctttcag cccacgtggg cctctgtggg ccgcgcatgg cctctcatgg
      781 cctcgcatgg cctcgcaggc cacaaaagcc gaagcccaaa gcccgctgtg ggggctctgg
      841 gcttcgagtg gagcagaggg ggatcgaacc cctgacctgt gcattgcgaa cgcaccgctc
      901 tcccagctga gctactgccc cgggacaggg ccgggaagct aaccgaactc cccccgcgac
      961 gcaagaactg aattacgcgg gcggatcttt tgccgcgctg gcccgcgatc tcaggcccag
     1021 gaccagcgcg agcaccacga cagccagcaa ggcgaggatc gggccgtcct ttggggcggt
     1081 cagccaggcg agccacgtcg gggcgagggc gagggtcatg aggggatgct caagcgggtt
     1141 cctgagggta caccacccgg cgcgcggtat aggggagcgg tggcgaggcc ctccaggacg
     1201 cggctcgatc agctgctcgt cgagcgcgag ctgtgcgagt cgcgggcgat cgcgcaggcg
     1261 ctggtggtcg cgggcaaggt cgtggtcggc gaccacaccg agaccaaacc cggcacgctc
     1321 gtgcccgccg acgcgcccgt gcgcgtgcgc gggcaagtcg cgccgttcgt gtcccgcggc
     1381 gggcagaagc tgcgcggcgc gctcgaccgc tttgcggggc tcgaggtccg cggacgcgtg
     1441 gtgatggaca tcggcgcctc gaccgggggg ttcaccgact gcgtgctgca ggccggcgcg
     1501 gaccgggtct acgcggtcga tgtcgggtat gggcaaatgg cgtggaagct ggcccaggac
     1561 ccgcgcgtgg tcgtgctcga ccgcaccaac atccgcacgc tggcgccgga gcgcatcggt
     1621 gagccggtga ctctggtggt cgcggactgc tcgttcatct cgctgcgcaa ggtgctgccg
     1681 gcggtgcccg cgttcctggc gccgcaggcg gacgtcgtgg cgctgatcaa gccgcagttc
     1741 gagctggcgc gcggggaggt cggcgccggc ggggtggtgc gcgacgccgg cgggcgcgca
     1801 gcggcgctgg cgggggtgct ggcggccgcg gaggcgctcg gctttcaggt cgtcgatacc
     1861 tgtgactcgg cgatcgcagg gcaggacggg aacctcgagc tgctcgcgtg gctgcgctgg
     1921 gacgtgggtt cgcaggctgt ccctcgggac agctcgtagg gatcgcgtga ggcgagcgca
     1981 agggggcgtg cgtgaagtcg cttgccgggg cgcggggcgg agtaattggc gtgctaagca
     2041 tcgcccccga tgcctctgcc gccgctcagc gcccgcgccc gtgctcacct caagtcgctc
     2101 gcccacgggc tggcgccgct cgttcaggtc ggcgccgatg ggatcacgcc aggcgtgggg
     2161 caggcggcgc tggtcgccct cgagcagcac gagctgatca aggtccggct cgggcagagc
     2221 tacgagggcg agcgggacga cgccgccagg gagctggtcg aggcgaccgg gtcgcagctg
     2281 gtccaggtga tcggccggat cgtggtcctg taccgccgcc gcagcaagga cgacccgaag
     2341 cgcccgcgca tcgtgatccc gacggccaag cccaaggccg gggcgagcaa ggccgccgag
     2401 aagccgggca agcaagcggg ctgagatcgc gcgtccggcg gcgattcgcg gcgctcgcgg
     2461 aggcgggccc tgcgagggta tgatgtaccc atgtccgcga cgcggcgcgc cctcctagca
     2521 gcctccgtgt tcgccgcgct cgtcggctgt aaaccgcgag ggacgaccca gccgagcaac
     2581 ccggccgtgt ggggcgagcc cggcaccggg gccggcagcc ggatcgagat ccagcgcagc
     2641 gaggcgccgg tcggggcgcc gtcgccggtg ctcgaggtgc tcgccggtga agtcgcgcgc
     2701 aacctcggcc agctgcagag cgaggaccgc gaggtcccgg cgtacttcct cgcgtacgac
     2761 ctgatcgcgc gcgatcacct gtggctcgag gtccagcagg gccaggtggt gcgccgccgc
     2821 ctcgacagcg accgcacgat cgacatcgat gtccgcgtcg gcagcccgca gctcgacaac
     2881 agccactcgc aaggcggcaa ctacgggccc ggcaacggcc tcggcgccgg ggagccggtg
     2941 acggtgcagg acgacccgct ggcgctcgcg caggcgctgt ggatgttcac cgaggagcag
     3001 taccgcgacg cgctcgtcgc cctgagcgcg gccgagagca acgagcaatt gcgcagtcag
     3061 gacgaggcca agccggcgag cccggacttc agcgaggaga cggcggtcgt gcacctcgag
     3121 ccgaaggtgc agctcgacct cgaggggctg cgcaaggcct gggagccgcg cttgaaagag
     3181 gcctcggtgg tgctcggtga cgacacgcgg gtgctcgaga gcaacgtggt gctgctggtc
     3241 aacgtcgaca accgcagctt cgtcgacagc aacggcagca agatccagag ctcgcatgtt
     3301 cgcttgcgga tcatgctcag cgcgaccgga caggccgacg acggcatgcg cctggctcgc
     3361 ttcgagagct tcgaggtgca cacgccggag cagctgccgg cgcaggcgct gctggtcgcc
     3421 acggcgaagc ggctgcgcga ggagctggtg gccctgcgcg ccgcgccgct ggccgagccc
     3481 tacgccgggc ccgcggtgct cgccggcagg gctgcggggg tgttttttca cgagatcttc
     3541 ggccatcgcc tcgaggggca ccggcaaaag gacgacctcg agggccagac cttctcgacg
     3601 atgatcggca agcgcgtgct gccggtgttc ctcgacatgg tcgacgaccc gaccgcgcag
     3661 agcctcggcg gggtgccgct cagcgggcac tattacgtcg acgacgaggc ggtgcccgct
     3721 cgccgcacga cgctggtcga acggggcaag ctcaaggggt tcctgctcgg gcgctcgcca
     3781 gtgctgccgt ttaaaaagag caacgggcac ggccggcgcg agcgcggtaa tcaggtggtc
     3841 gcgcgccagg gcaacctgat cgtcaccagc cgcaagacga tccccgacgc ccagctgcgc
     3901 aggactttga ttgaggaggt ccagcgccag ggcaagccgt acgggctgtg gttcagcgac
     3961 attcagggcg gttacacgat caccgagcgt agtggtcccc aggcgttcaa ggtgatgccg
     4021 ctgctggtgt accgcgtgtg gaccgacggg cgcccggacg agctggtgcg cggcgtcgac
     4081 atcgtcggca caccgatcgc ggcgttcgag acgatcatcg ccaccggcga ctcgcccgga
     4141 atttttaatg ggatgtgcgg ggccgagtcg ggcgacgtgc cggtctcggc ggtcagcccg
     4201 tcgctgctgc tgcgcagcct cgagatcgag cgcgcgagcc atgaccgcga caagccgccg
     4261 ctgatgcccc cgcccgagac tttattaaag aatctgtccc gcgggacagg gagcgaggtg
     4321 gcccgatgaa ggcggcgctc ctgatcttgc tcgccgcgct ggccgacgcg ccggcgaaca
     4381 ccgagacgac cgctaagccg accgcgaaga ccgagccggt gaagccggcg aaggccgagg
     4441 cgccgccggc gagatcggcg aaggccgagg tgccagcgaa gccggcggtg agggagatca
     4501 agccgctggg caagcgcgcg ctcgacctcg aggtcgcggc gatcgagcag gagctggcgc
     4561 gcggggtcgg agggctgcgc ctgccggact cgccggcgcc ctacctggcg acggtgcaga
     4621 tcatccgcgc atcggtgctc agcctcgatg gatcgtacgg cggcgtgatc accgatgtcg
     4681 tcgaggagca ggcggtcgcg gcggtcgagg tgcgggtcgg cagcccggcg cgggaccaga
     4741 gcggcacctt cggcaacgac ggggcgcggc tgcgcttcat catctcgctc gagccctcgc
     4801 cggtgctcag ccgccataaa ctctggctgg cgctcgacca ggactttcgc gccgcgacgg
     4861 cgctgttcgc ccagaagcag gcgatcctgg cccgcctcgc cggtgagccg cccccgccgg
     4921 acctcggccc gaagccggag ccgatgccgc gccagccggc ccccgacgag ggctcgccgc
     4981 agctcgaccg cgagggcctg cacacgatgg tgatgcagct gagcaagcgc ttcgtcgacc
     5041 acccggagat cgacaacggc gacgtgttcg tgcaggtgct gcgcacgacg gtgacgacga
     5101 tcaattccga ggggctggtg ctgcacgagc gccacgaccg ggcggtgctc gcggtggtcg
     5161 ccgatacgcg cgcggccgat ggcatgcacc tcgacgcggg cgctgcgatc catttgcaag
     5221 agctg
//
LOCUS       DASWNJ010001445         2674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_447809, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001445 DASWNJ010000000
VERSION     DASWNJ010001445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447809"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(540..1241)
                     /locus_tag="VGB85_24055"
     CDS             complement(540..1241)
                     /locus_tag="VGB85_24055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137187.1"
                     /translation="MRTGRRRLALAFAALSALPALPVTALVAGALVVAAPGCARKQSR
                     GDLAPPADAAAWDPLHDIIFDDHYTQTPLELSGRAPGDVTDQRRLAQRLGFADIVALV
                     TVDQVWSRGLHDGVPRQRVDVTIDRVMLGALPRGTRKDQVLELRGGEDLPAALTGRVM
                     LMFLRWAPGEGPGYHHHLMPADDNVLAWVTAMVRHARALGKLDAVEKPRKRRRGRSGA
                     SATSDAPAESDAPAG"
     gene            1274..1954
                     /locus_tag="VGB85_24060"
     CDS             1274..1954
                     /locus_tag="VGB85_24060"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0137188.1"
                     /translation="MRTSWIGACLLAACALGACALGAPRPFAPPVPEVVAANQLAGDP
                     HAGRFPYEEAVAGLPEGPTLRALIDTDAGEIHCRLDPGSAPIAVASFVGLARGLRAFQ
                     AAPGEPWQTGPFFDGLPFHRAVERQFVQTGRRGQENAGFRLQDEMSSGHVFDRAGLMA
                     LANAGTPHSSSAQFFITTEPLAQLDGKHTIFGACDDEDVVRELERRVLATPAAPPTIR
                     RVTITRGS"
     gene            2048..>2674
                     /locus_tag="VGB85_24065"
     CDS             2048..>2674
                     /locus_tag="VGB85_24065"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0137189.1"
                     /translation="MSTSLARTLLFTTAFLASACTAPDAPAQKAEPKLEAKPEPAAQT
                     ETKADVEAKAKAEPAAPVAKFRKGQIARPGMSPEDLLEYNKAQGDPMGSIDLKTALAG
                     DPALEDTSKGKLFATFDTTMGSFRCELYEDKTPNTVANFVGLARGVRPFYDKKSDAWK
                     TAPFYDGILFHRVIPGFMIQTGDPLGTGTGGPGFLITDEFDKSLRHVGP"
BASE COUNT          379 a         1001 c          885 g          409 t
ORIGIN      
        1 gaacttgtgc tctcatcatg atcagggact cgttagaatc gcctgaccgg ttgtcggtgt
       61 cgctacctcg cggctgcgac ccgacggggt gtggattcaa agatccacgt acccacaagc
      121 gtcatttggt ttccctcttc cgctatccag ttttcaggga gccagtccgt gaggactttt
      181 ccgtcgccgt ctcaggtagt cgccgctttc gcggcggcgc cgtcagtggc gagcgggagg
      241 cggtttctac tcttcgccgc ccagcgagtc aacatctttt tttcggggtc tcgttttttt
      301 ctgagcttgt cgtgctcatg tccgccccgg aaaggattgg acgacctcgg cgcaattttt
      361 cggggatcca acctcgaaag gttgggttcc gaccgggagc gttgtccccg aggcgggctc
      421 gctcccgcgg agaaggatat agctactgac ccgggtcccc gacaagcccc cgcaatggga
      481 tcccagctca cactttttcg cgccgggggg tcgccgtgga acgcgccggg cgcccgccct
      541 caacccgcgg gcgcgtcgga ctcggccggc gcgtcgctcg tcgcagacgc gcccgaacgg
      601 ccccgacggc gctttcgcgg cttctccacg gcatcgagct tcccgagcgc acgcgcgtgg
      661 cggaccatcg ccgtcaccca cgcgagcacg ttgtcgtcgg ccggcatcag gtgatgatgg
      721 tagccggggc cctcgccagg ggcccagcgc aggaacatca acatgacccg cccggtcagc
      781 gcggcgggga gatcttcacc cccacgcagc tcgagcacct ggtccttgcg cgtgcccctg
      841 ggcagcgccc cgagcatgac ccggtcgatc gtcacgtcga cgcgctggcg cgggacgccg
      901 tcgtgcaggc cgcgactcca cacctggtcg accgtcacca gcgcgacgat gtccgcgaag
      961 ccgagccgct gggccaggcg tcgctgatcg gtgacgtccc ccggcgcccg ccccgagagc
     1021 tcgagcggcg tctgcgtgta gtggtcgtcg aagatgatgt cgtgcagcgg atcccaggcg
     1081 gctgcgtcgg ccggcggcgc caggtccccg cgggattgct tgcgggcgca gcccggcgcg
     1141 gccaccacca acgcccccgc caccagcgcc gtcaccggca gtgccgggag cgccgagagg
     1201 gctgcgaagg cgagggccag ccgtcgtctg ccggtgcgca tgtcgggcgc gagagtagcg
     1261 tatgctccgc cccgtgcgca cctcgtggat cggcgcctgc ctgctcgccg cctgtgccct
     1321 cggggcctgt gccctcggcg ctccccggcc gttcgcgccg ccggtgcccg aggtcgtcgc
     1381 cgccaaccag ctggccggag atccccacgc cggccgcttt ccttatgagg aggcggtcgc
     1441 cgggctcccg gagggcccga ccctgcgcgc cctgatcgac accgacgccg gcgagatcca
     1501 ctgccgcctc gatccgggca gcgcgccgat cgcggttgcc agcttcgtcg gcctggcgcg
     1561 cgggctccgg gcgttccagg ccgccccggg cgagccctgg cagaccggcc cgttcttcga
     1621 cggcctcccg tttcaccgcg ccgtcgagcg gcagttcgtc cagaccggcc gccgcggcca
     1681 ggagaacgcc ggctttcgcc tgcaggacga gatgtcgagc ggccacgtgt tcgaccgcgc
     1741 cggcctgatg gcgctcgcca acgccggtac gccgcacagc agctcggcgc agttcttcat
     1801 cacgaccgag ccgctggccc agctcgacgg caagcacacg atcttcggcg cctgcgacga
     1861 cgaggacgtc gtccgcgagc tcgagcgccg cgtgctcgcc accccggccg ccccgccgac
     1921 gatccggcgc gtcacgatca cgcgcgggtc gtgatgtttt tcacgctttc ctgacacgcc
     1981 gggccccggc ggtcgtcggc tgcgccggac gcttgtcccg gggctcgctc ggtggcaaaa
     2041 cgccgccatg tcgacctcgc tcgcgcgcac gctcctgttc acgaccgcct tcctggccag
     2101 cgcctgcacc gcgcccgacg ctccggcgca gaaggccgag cccaagctcg aggccaagcc
     2161 cgagcccgcg gcgcagaccg agaccaaggc cgacgtggag gccaaggcga aggccgagcc
     2221 ggcggccccg gtcgccaagt tccgcaaggg tcagatcgcc cgccccggca tgagcccgga
     2281 ggacctgctc gagtacaaca aggcccaggg cgacccgatg ggctcgatcg acctgaagac
     2341 cgcgctcgcc ggcgacccgg cgctcgagga caccagcaag ggcaagctgt tcgcgacctt
     2401 cgacacgacg atgggctcgt ttcgctgcga gctctacgag gacaagacgc cgaacaccgt
     2461 cgccaacttc gtcggcctcg cgcgcggcgt gcggccgttc tacgacaaga agagcgacgc
     2521 ctggaagacg gcccccttct acgacggcat cctgttccac cgcgtgatcc ccgggttcat
     2581 gatccagacc ggcgacccgc tcggcaccgg cactggcggc cccggcttcc tgatcaccga
     2641 cgagttcgac aagagcctgc gccacgtcgg cccg
//
LOCUS       DASWNJ010001446         2236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_447852, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001446 DASWNJ010000000
VERSION     DASWNJ010001446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447852"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VGB85_24070"
     CDS             complement(<1..501)
                     /locus_tag="VGB85_24070"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEY0137190.1"
                     /translation="MAEPDRERRARVESLVRELCSPACAGRAPGTPEGIAAREVVVRA
                     MTSAGVAPGGTDGYLQTVPRCGANVLGELPGAGPLADRTVVVAAHYDHLGKAGGGQAY
                     WGADDNAAAVAILVETGRVLASRGAGGRRVVLAAYDGEEPPHFMQGTMGSMYHVAHPT
                     APLASID"
     gene            781..1653
                     /locus_tag="VGB85_24075"
     CDS             781..1653
                     /locus_tag="VGB85_24075"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0137191.1"
                     /translation="MLANDRSLVGPDGGLIAYRVEGRGPAMVLTNGITTSNVFWTYLH
                     RRWSSRYTVINWDLKGHGRSAPARSPGAASIPALAEDLLRVLDAAGSERATLVGFSMG
                     CQVCLEAYRRAPERVNALVLLLGGAGRVFDHALGPVGKLLHGSIARMSARGFSISLRS
                     LQRVVASPIAYRLGRGLRLLGHEARRADVQRMVDHFVRDIDPATLSAMALAAQEHSAE
                     DLLPSITVPTLIVAGDRDVFAPGSHVAGLQTKIPGSELLRLPYGTHTSLVEHHAEIGA
                     AVEDFLRRRLSSVT"
     gene            1711..>2236
                     /locus_tag="VGB85_24080"
     CDS             1711..>2236
                     /locus_tag="VGB85_24080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137192.1"
                     /translation="MLRRALVLSLILVACGGNPDSETTVDSTGGTTTAGAGSSTSDVT
                     PTTSGASTSDTGDTGPGPAIDYSVAGPHPVGNTRFVLPAGERELLVELWYPADAGAAE
                     LAAQGHPIAEFVPAGPDRVAFDDLLTKLSPAGQIGTRLQTRSALDATPAAGGPWPVLV
                     FSHCFDCVRFSAFSV"
BASE COUNT          276 a          843 c          795 g          322 t
ORIGIN      
        1 gtcgatgctc gccagcggcg cggtcgggtg ggccacgtgg tacatcgagc ccatcgtgcc
       61 ctgcatgaag tgcggcggct cctcgccgtc gtaggccgcg agcacgaccc gtcgcccgcc
      121 ggcgccgcgg ctcgcgagca cgcgaccggt ctcgacgagg atcgccaccg cggccgcgtt
      181 gtcgtcggcg ccccagtacg cctggccgcc gccggccttg ccgaggtgat cgtagtgcgc
      241 cgcgaccacg accgtgcggt ccgccagcgg tccggcgccg ggcagctcgc cgagcacgtt
      301 ggccccgcag cgcggcacgg tctgcaagta cccgtcggtg ccgccgggcg cgacgccggc
      361 ggaggtcatc gcacgcacga cgacctcgcg cgcggcgatg ccctcgggcg tgcccggggc
      421 tcgaccggcg caggcgggcg agcacagctc gcggacgagg gactcgacgc gggcgcgtcg
      481 ctcacggtct ggctcggcca tgccgcttaa ttacacgagt cggccggtgg tgcacgcccg
      541 gccgaccgcg agccgccgct cgctgcgggt cacgtgatcg cctgtcgctc gcgcgccgcc
      601 tcgcgtcgcc gaccgacacc aggcgcgagc gtcgaccgac tcgcgtcgcg cctgtcgaat
      661 tacccaccgt ctcgcgtcgc accggccacg caggtgctgg gccgcgtgag cgtcgttccc
      721 ggaccggccc ggccatggac gaagcgttgc cggggtcgag gatctgcgat atgagagatt
      781 gtgctggcca atgaccgcag cctcgtcggc cccgacggcg gcctgatcgc ctaccgggtg
      841 gaggggcgcg ggcccgcgat ggtgttgacc aacgggatca cgacgtcgaa cgtcttctgg
      901 acgtatctgc atcgacgctg gtcgagccgt tacacggtca tcaactggga cctcaagggc
      961 cacgggcggt cggcgccggc gcgcagcccc ggggccgcgt cgatccccgc gctcgcggag
     1021 gacctgctgc gggtgctcga cgcggcgggc agcgagcggg cgacgctggt cggattttcg
     1081 atgggctgcc aggtctgcct cgaggcctac cggcgggcgc ccgaacgagt gaacgcgctg
     1141 gtgttgctgc tcggcggggc cggtcgcgtg ttcgaccacg cgctcggccc ggtcggcaag
     1201 ctgttgcacg ggagcatcgc gcgcatgtcg gcgcgcggct tctcgatcag cctgcgcagt
     1261 ctgcaacggg tcgtcgcctc gccgatcgcc tatcgcctcg gtcgcggcct gcggctcctg
     1321 ggacacgagg ccaggcgggc ggacgtgcag cgcatggtcg atcacttcgt ccgtgacatc
     1381 gaccctgcca ccctctcggc gatggcgctg gcggcccagg agcactccgc ggaggacctg
     1441 ctgcccagca tcacggtgcc gaccctgatc gtcgccggcg accgcgacgt gttcgcgccg
     1501 ggctcgcatg tcgcggggtt gcagaccaag atccccggca gcgagctgct gcggctgccg
     1561 tatggcaccc acaccagcct cgtcgagcat cacgcggaga tcggggccgc cgtcgaggac
     1621 ttcctgcgcc gccggctttc gtccgtgacc tgacctcggg gacaggtcgc gggccttcca
     1681 cgcgcgggcg ctttcggcgt atacggcgtc atgctccgcc gcgcgctcgt cctctcgctg
     1741 atcctcgtcg cttgcggggg caaccccgac agcgagacca ccgtcgacag cacaggcggc
     1801 acgaccacgg ccggcgccgg cagcagcacc tccgacgtca ccccgaccac cagcggcgcg
     1861 agcaccagcg acaccggcga caccggcccg ggcccggcga tcgactactc cgtcgccggt
     1921 ccgcacccgg tcggcaacac gcgcttcgtg ctgcctgccg gcgagcgcga gctgctcgtc
     1981 gagctgtggt acccggccga tgccggcgcc gccgagctcg ccgcgcaagg ccacccgatc
     2041 gccgagttcg tgcccgcggg cccggaccgc gtcgccttcg acgatctgct cacgaagctc
     2101 tcgccggcgg ggcagatcgg cacgcggctg cagacccgct ccgccctcga tgccacgccc
     2161 gccgcgggtg gaccgtggcc ggtgctcgtg ttctcgcact gcttcgactg cgtgcgcttc
     2221 tccgcgttct ccgtcg
//
LOCUS       DASWNJ010001447         3985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_484839, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001447 DASWNJ010000000
VERSION     DASWNJ010001447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484839"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VGB85_24085"
     CDS             complement(<1..402)
                     /locus_tag="VGB85_24085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137193.1"
                     /translation="MRLRAFALASLSLSACQSPVEGGEASESAGSSGASESGSSGETP
                     TTTPTTTASTGDSDDGGATCPMCGGDEACIAGECVAVGRGEVERGCSVLGDPGGRGQC
                     LYPWPSDLWTVADATSETGRRLAHDPELLPKN"
     gene            complement(523..1023)
                     /locus_tag="VGB85_24090"
     CDS             complement(523..1023)
                     /locus_tag="VGB85_24090"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEY0137194.1"
                     /translation="MNLANPTYICLDVPEPQASQVMAWRERYCERLRAFPVELTIAGS
                     SGLGAIRPQLDPRDVEARLRKLCRETAPISAQFGTVVRFPDTDIFCLSLADPMPFEAI
                     HDGLKQAGVRFEPSRFPFFPHCTLRMAGPLAPGAVNELFSVRVPGTFVLGTMSLYQRE
                     PDDTIR"
     gene            1256..2989
                     /locus_tag="VGB85_24095"
     CDS             1256..2989
                     /locus_tag="VGB85_24095"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEY0137195.1"
                     /translation="MFRRPLALASLLLAGCYTGADLSPDSGETAVTGVTGNSGNSGAD
                     DGTPTTGSGEDAGESAGDDGADPADFDPAPVRLRLLLARHYQNTVRDLLGDAAAGLAA
                     PPTDTALNGFEAIAAAQVSLTDSAVEVYEKSARAVAAEAIKDQARIAGYLGCAPTGPD
                     DVNCHRQFVTNFGRMIWRRALTTEEVELYTAVAQEAALQFGNFDVGVEAAIATFLQSP
                     YFLYQVEVGEPDPDEPSDRNLTSVELATRLSYFLLDTTPSAELLALAEGGGLSTPDQV
                     RAVAELMVEQPGARQAIGNFYAEVLKLRALEELPKDPTLYPQFSPSLAAAMRQETLAL
                     IDDIAFNVDGDFRDILNAPYTFVNGPLAALYGLIPDPNQLGDTWQRFDLPVEGKRGGI
                     LGQSAFLAAYAHISSTSPTLRGKFVREALMCQGMPAPPPDVVTELPAGEEYKTMRERL
                     SKHKKVECAGCHDLMDPIGLGLENFDGIGIFRLVENGVTLDTSEEVLGLGSFDGAREL
                     GTILRDSPDVTRCMVRNLFRHATGHLELLSEKVALDEVDGAFASAEFRLKDLLVELVS
                     SPAFLRVGTPE"
     gene            3022..>3985
                     /locus_tag="VGB85_24100"
     CDS             3022..>3985
                     /locus_tag="VGB85_24100"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEY0137196.1"
                     /translation="MSRFSLSRRTMLRGAIGGTSVALALPLLDAMLNSNGTALAAGEP
                     LPRRLVTWFFGNGVALNDVVNPGAGLRWGPAEQGPNYPLTPQLSGLEGVQEYCSILTG
                     FDIKAAGQHRRGHHDGVAGFFSGHPFIELPHADNSYSSKSGGPTIDQVAAAAIGNQTF
                     LPSVQIAVSKRVITSEGPTLQFLSHKSADEALPQIFNPKDLYTKLFDSFVPQDDPTKP
                     LRLNALDAVAEDVKRLKMRVGMADRIRLDAHLSSVEQIRKQIDALAPECMQPMMPVNG
                     NDDFEGKEPLDEVNQIMADLLVEAFRCDITRIASMQYTGSVGYTV"
BASE COUNT          615 a         1580 c         1217 g          573 t
ORIGIN      
        1 gttcttcggc agcagctccg ggtcgtgcgc cagccgacgg cccgtctcgc tcgtcgcgtc
       61 ggcgaccgtc cagagatcgc tcggccacgg atacaggcac tgcccgcggc ccccggggtc
      121 cccgagcacg ctgcacccgc gctcgacctc gccgcgcccg accgcgacgc actccccggc
      181 gatgcacgcc tcgtcgccgc cgcacatcgg acaggtcgcg ccgccgtcgt cgctgtcgcc
      241 cgtgctcgcc gtggtcgtcg gcgtggtggt cggcgtttcg ccgctcgagc ccgactcgct
      301 cgcccccgac gatcccgcag actcgctggc ctcgcccccc tcgacgggcg actgacacgc
      361 ggacagggag agcgacgcga gggcgaaggc acgaaggcgc atcgaagatc tctaccacca
      421 tcccgggacg gcgatgcgcg atattaattc gtccccgacc gcgtcaacca gtcatccggt
      481 catccggtca accggtcatc cggtcagcgc ttcggaccac acctagcgga tcgtgtcgtc
      541 gggctcgcgc tgatacagcg acatcgtgcc gagcacgaac gtgccgggca cgcgcaccga
      601 gaacagctcg ttgaccgccc cgggcgccag cggcccggcc atgcgcaggg tgcagtgcgg
      661 gaagaacggg aaccggctcg gctcgaagcg cacccccgcc tgcttgagcc cgtcgtggat
      721 cgcctcgaag ggcatcgggt ccgcgaggct gaggcagaag atgtccgtgt cggggaaccg
      781 aaccaccgtg ccgaactgcg ccgagatcgg cgccgtctca cggcacagct ttcgcagcct
      841 cgcctcgaca tcgcgcggat cgagctgagg tcggatcgcc ccgagcccgc tcgagcccgc
      901 gatcgtcagc tcgaccggga acgcacgcag ccgctcacaa tagcgctcgc gccaggccat
      961 cacctgcgac gcctgcggct cgggcacgtc caggcagatg tacgtggggt tggccaggtt
     1021 catcgcggct cgcgttccgc ggttttagcg gatcgacggc cggcgtcgaa tgaaaacttt
     1081 cgcccgcgcc gcgcgcgcgg cgtcgagagc ccgacaaagc gcccgcgaac gccgtggcca
     1141 atgcacccct gaggcccgcc agacgcccgc tccgcccccg ccatgggggt tgaaggagac
     1201 cgtagactgc cacttctgcc tgtggctatg gtgccgtctc gtggaggtcc tgcccatgtt
     1261 tcgtcgtccc ctcgcgctcg cctcgctgct cctcgcgggc tgttacaccg gcgcggatct
     1321 ttctccggac tccggtgaaa ctgccgtcac cggcgttacc ggcaactctg gcaattcagg
     1381 cgcggacgac ggcacgccga cgaccggctc gggtgaggac gccggtgagt ccgctggtga
     1441 cgacggcgcc gatccggccg acttcgaccc ggcgccggtc cgcctgcgcc tgctgctcgc
     1501 ccgtcactac cagaacacgg tccgcgacct gctcggcgac gccgcggccg gcctcgccgc
     1561 cccgccgacg gacaccgccc tcaacggctt cgaggcgatc gccgcggccc aggtctcgct
     1621 gaccgactcc gccgtcgagg tctacgagaa gtccgcgcgg gccgtcgccg ccgaggcgat
     1681 caaggatcag gcccgcatcg ccggttacct cggctgcgcc ccgaccggcc ccgacgacgt
     1741 caactgccac cgccagttcg ttactaactt cgggaggatg atctggcgcc gcgccctgac
     1801 gaccgaggag gtcgagctct acaccgccgt cgcccaggag gccgcgctgc agttcggcaa
     1861 cttcgacgtc ggcgtcgagg cggcgatcgc caccttcctg cagtcgccct acttcctcta
     1921 ccaggtcgag gtcggcgagc ccgaccccga cgagcccagc gaccgcaacc tgacctcggt
     1981 cgagctcgcc acccgcctgt cctacttcct cctcgacacc accccgagcg ccgagctgct
     2041 cgcgctcgcc gagggcggcg gcctgtcgac gccggaccag gtccgcgccg tcgccgagct
     2101 gatggtcgag cagccgggcg cccgccaggc gatcggcaac ttctacgccg aggtgctcaa
     2161 gctgcgcgcg ctcgaggagc tgcccaagga cccgaccctg tacccgcagt tctcgccgtc
     2221 cctcgccgcc gcgatgcgcc aggagaccct cgcgctgatc gacgacatcg ccttcaacgt
     2281 cgacggcgac ttccgcgaca tcctcaacgc gccctacacc ttcgtcaacg gcccgctcgc
     2341 cgccctctac ggcctgatcc ccgaccccaa ccagctcggc gacacctggc agcgcttcga
     2401 cctccccgtc gagggcaagc gcggcggcat cctcggccag tcggccttcc tcgccgccta
     2461 cgcgcacatc tcgagcacct cgccgaccct gcgcggcaag ttcgtgcgcg aggccctgat
     2521 gtgccagggc atgcccgcgc cgccgcccga cgtcgtcacc gagctgcccg ccggcgagga
     2581 gtacaagacg atgcgcgagc gcctctcgaa gcacaagaag gtcgagtgcg ccggctgcca
     2641 cgacctgatg gacccgatcg gcctcggcct cgagaacttc gacggcatcg gcatcttccg
     2701 gctcgtcgag aacggcgtca ccctcgacac ctcggaagag gtcctcggcc tcggcagctt
     2761 cgacggcgcc cgcgagctcg gcacgatcct tcgtgacagc cccgatgtca cccgctgcat
     2821 ggtccgcaac ctgttccgcc acgccaccgg ccacctcgag ctgctgagcg agaaggtcgc
     2881 cctcgacgag gtcgacggcg ccttcgccag cgccgagttc cgcctcaagg acctgctcgt
     2941 cgagctggtc agcagccccg ccttcctccg cgtcggcacc cccgagtgag acccccgtcc
     3001 ttgactccca ggagacacac catgagccgc tttagcctca gccgtcgcac gatgctccgt
     3061 ggcgccatcg gcgggacctc ggtcgccctc gccctcccct tgctcgacgc catgctcaac
     3121 agcaacggca ccgccctcgc cgccggcgag ccgctgccgc gccgcctcgt cacatggttc
     3181 ttcggcaacg gcgtcgccct caacgatgtc gtcaaccccg gcgccggcct gcgctggggc
     3241 cccgccgagc agggcccgaa ctacccgttg accccgcagc tgtcgggcct cgagggcgtg
     3301 caggagtact gctcgatcct caccggcttc gacatcaagg ccgcagggca gcaccgccgc
     3361 ggccaccacg acggcgtcgc cggcttcttc tccggccacc ccttcatcga gctgccgcac
     3421 gccgacaaca gctactcctc gaagtcgggc ggcccgacga tcgaccaggt cgccgcggcc
     3481 gcgatcggca accagacctt cctcccctcg gtccagatcg ccgtgtcgaa gcgcgtgatc
     3541 accagcgagg gcccgaccct ccagttcctg tcgcacaaga gcgccgacga ggccctcccg
     3601 cagatcttca accccaagga cctgtacacc aagctgttcg acagcttcgt cccgcaggac
     3661 gacccgacca agccgctgcg cctcaacgcc ctcgacgccg tcgccgagga cgtcaagcgc
     3721 ctcaagatgc gcgtcggcat ggccgaccgc atccgcctcg acgcccacct gagctccgtc
     3781 gagcagatcc gcaagcagat cgacgccctc gcgcccgagt gcatgcagcc gatgatgccc
     3841 gtcaacggca acgacgactt cgagggcaag gagccgctcg acgaggtcaa ccagatcatg
     3901 gccgacctgc tcgtcgaggc cttccgctgc gacatcaccc gcatcgccag catgcagtac
     3961 accggctcgg tcggttacac cgtgt
//
LOCUS       DASWNJ010001448         3187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_274240, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001448 DASWNJ010000000
VERSION     DASWNJ010001448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274240"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..1302
                     /locus_tag="VGB85_24105"
     CDS             103..1302
                     /locus_tag="VGB85_24105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137197.1"
                     /translation="MTAGKPREPPEPAPEPSGSSGAWPADAGLSSLLRRSVKAAIARW
                     AVRRRSDTEIWPRPQDEAMHPWDGQRRFAEEYTQVAVQPGLAVIARIEWLPGREAHRV
                     WLTLLTPTAVYALPGTGQALLRGDSDHWRAGGVAFDCVEPLRTWTVRYRGRLEVRDPR
                     GHRRRPTAASDELPVEDLEVRVDLTFLSQVPAFVPGSDDDADLMSRQLGAAEWDARLL
                     RVLRRHTLRSYVQAGEVHGTVALGPNLLAFRGAGLRQHSWGVRDWGASDEATQCFASF
                     TQDGAAREAADKPVQVWLHSARFPWLTLAGGFVQRAAGLVAIRDLGVRPTRQPARAPS
                     HVGFHVDAPGGDIDLETETVAELGLDMDGRGHLDLALCKVRGSGEGWALVISQRRLLR
                     RPTATEE"
     gene            1367..1780
                     /locus_tag="VGB85_24110"
     CDS             1367..1780
                     /locus_tag="VGB85_24110"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY0137198.1"
                     /translation="MPIQRLSSREAIERVASDAALVHIDVRSEPEFTAGHAPGAYNVP
                     LMHMGPAGMTPNPNFTAEVERAFPDKDTPIMVSCKAGGRSAKAAAALEALGYRQLADH
                     IGGWGGGNGDPGWVAAGGPSATQTQPGRSYPELKA"
     gene            1777..2190
                     /locus_tag="VGB85_24115"
     CDS             1777..2190
                     /locus_tag="VGB85_24115"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0137199.1"
                     /translation="MSATTLPQTLGLRHVAVSIAGEGFDACARFYTEGMGMAVDWKPD
                     ADNLYLTYGGDNLALHRVPEIDHRVSALDHLGFCTPTADDVRAWHDRIVSLMDTHTLE
                     IVQPPKLHRDGSTSFYFRDPAGNKVQVIHIPSLRG"
     gene            2890..>3187
                     /locus_tag="VGB85_24120"
     CDS             2890..>3187
                     /locus_tag="VGB85_24120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137200.1"
                     /translation="MDTDASPSTGTTGAAADTTTDAVTSSPDPTGGMAEMTTTTLSST
                     TTAGDDLTTGGSTTGGSTTGLDATTTDIDSTGSSTTGPDATCGNGILEPGELCDD"
BASE COUNT          456 a         1209 c         1089 g          433 t
ORIGIN      
        1 cggagcacgg ggtacctccc ggaaccggag ttatcacggg cgcgggtgca attgctacgc
       61 cctggcggcc tccggcgccg acctgctagg ctgcgcgttg cgatgaccgc tggcaagccc
      121 cgcgagcccc ccgagcccgc acccgagccg tccgggtcat ccggagcatg gcccgcggac
      181 gccggcctgt cgtcgctcct gcgccgcagc gtcaaggccg cgatcgcgcg ctgggcggtg
      241 cgccgccgca gcgacacgga gatctggccg cgcccgcagg acgaggcgat gcacccctgg
      301 gacggccagc gccgcttcgc ggaggagtac acgcaggtcg ccgtgcagcc cggcctggcg
      361 gtgatcgccc gcatcgagtg gctgcccggg cgcgaggcgc accgcgtgtg gctgaccctg
      421 ctgaccccga cggccgtgta cgcgctgccc ggcaccgggc aggcgctgct gcgcggcgac
      481 agcgaccact ggcgcgccgg cggcgtcgcg ttcgactgcg tcgagcccct gcgcacctgg
      541 accgtccgct accgcggccg cctcgaggtc cgcgatcccc ggggccaccg ccgccgaccc
      601 accgccgcga gcgatgaatt gccggtcgaa gatctcgagg tgcgcgtcga cctcacgttc
      661 ctctcgcagg tccccgcgtt cgtgcccggc agcgacgacg acgccgacct gatgtcgcgc
      721 cagctcggcg ccgccgagtg ggacgcccgc ctgctcaggg tcctgcgccg acatactttg
      781 cgcagctacg tgcaagccgg cgaggtccac ggcaccgtgg cgctcggccc gaacctgctc
      841 gcgtttcgcg gcgccggcct gcgccagcac agctggggcg tgcgcgactg gggcgccagc
      901 gacgaggcca cccagtgctt cgccagcttc acgcaggacg gcgcggccag ggaggccgcc
      961 gacaagccgg tgcaggtgtg gctgcacagc gcccgcttcc cgtggctgac gctcgcgggc
     1021 ggcttcgtgc aaagggctgc cggcctggtc gcgatccgcg acctcggcgt gcgaccgaca
     1081 cgccagcccg cgcgcgcgcc cagccacgtc ggcttccacg tcgacgcccc cggcggcgac
     1141 atcgacctcg agaccgagac ggtcgccgag ctcggcctcg acatggacgg ccgcggccac
     1201 ctcgacctcg cgctgtgcaa ggtccgcggg tccggcgagg gctgggccct cgtcatctcg
     1261 cagcgcaggc tcctgcgccg cccgaccgcg acggaggagt gaggcgcgag cacctgtccc
     1321 cggggacagg tggacaccgc gcgggacctg gactagggtt gcgcccatgc cgatccagcg
     1381 cctgagctcc cgggaggcga tcgagcgggt cgccagcgac gccgccctcg tccacatcga
     1441 cgtccgctcc gagcccgagt tcaccgccgg ccacgcgccc ggggcctaca acgtgcccct
     1501 gatgcacatg ggccccgccg gcatgacccc caacccgaac ttcaccgcgg aggtcgagcg
     1561 cgccttcccc gacaaggaca cgccgatcat ggtgtcgtgc aaggccggcg gccgctcggc
     1621 caaggccgcc gcggcgctcg aggccctcgg ctaccgccag ctcgccgacc acatcggcgg
     1681 ctggggcggc ggcaacggcg atcccggctg ggtcgccgcc ggtggcccga gcgcgaccca
     1741 aacccagccc ggccgcagct acccggagct caaggcatga gcgccacgac cctcccgcaa
     1801 acgctcggcc tgcgccacgt cgccgtgtcg atcgccggcg agggcttcga cgcctgcgcc
     1861 cgcttctaca ccgaaggcat gggcatggcc gtcgactgga aacccgacgc cgacaacctc
     1921 tacctgacct acggcggcga caatctggcg ctgcaccgcg tccccgagat cgaccaccgc
     1981 gtgtccgccc tcgaccacct cggcttttgc accccgaccg ccgacgacgt ccgcgcctgg
     2041 cacgaccgca tcgtttcgtt gatggatacc cataccctcg agatcgtgca gcctccgaag
     2101 ctgcatcgcg acgggtcgac ctcgttctac tttcgtgatc cggccgggaa caaggtgcag
     2161 gtgatccata ttccgtcgct gcggggttga gcggcgcgct cgggtgccgg tgttgttcgg
     2221 gcgaaccgcc tggcgtgttc gggctcgggt agtgctcgga cgaacggctg gcgtgttcgg
     2281 agagcgcggg gcgaggctat ctgggccggg gggggccgcc ggacggcggg tccctgcgac
     2341 gcgggtcctc ccaggggacg tgctcgccgc cccatctgtc acgcacaaca tatcgcttac
     2401 gccaagactg cctctcacga ccggaagttg acggggcgtc tgcgcgcgac ccctgggtcc
     2461 tcccgctgct tcccccgctc gtcggcccct gaccccccgg gcgaacgttg tctgcgcccc
     2521 gctcgtcaca gggacaaaac tgtcgcgcaa ccgggagact cgcacaggcg cctgggtccg
     2581 ggcctccggc aagtgcgacg cctgggtcat cgacggtagc gacgtgcagc ccgggcactg
     2641 cgacggtcgc ggcgcgaggc gcaggactcg atacattgcc gcctcgctca tcaagccgcg
     2701 gatccggatg gcgggttcgg cttatggacg ggtgagggcc gatgtgagag cttcagggag
     2761 aagatgtcga tgatcacgcg aacacatttt atgcacgatc gaacgcttgg tccgtcctct
     2821 cttcgtctcg cttgccgggc gccgttcgcc gcgttgttgt tgggggcgtg cccgctcggg
     2881 ccgcaagcca tggacacgga cgcctcgccc tcgacaggca ccaccggcgc cgccgcggat
     2941 acgaccaccg acgccgtcac gtccagcccg gaccccaccg gcggcatggc cgaaatgacg
     3001 accaccacgc tatcgagcac cacgaccgcc ggcgatgacc tgaccaccgg gggctcgacc
     3061 accgggggct cgaccaccgg gctcgacgcg acgaccaccg acatcgacag taccgggagc
     3121 tcgaccaccg gcccggacgc tacgtgtggt aacggtattc tcgagcctgg cgagctctgc
     3181 gatgacg
//
LOCUS       DASWNJ010001449         2460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_408091, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001449 DASWNJ010000000
VERSION     DASWNJ010001449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408091"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(590..1597)
                     /locus_tag="VGB85_24125"
     CDS             complement(590..1597)
                     /locus_tag="VGB85_24125"
                     /inference="COORDINATES: protein motif:HMM:NF014951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137201.1"
                     /translation="MAKPLKYLITADPIASFQPRFDTTLRLMAELRARRAVVDYCDLL
                     ATDTALPGPRYLAALPVHAVLDADPTRVPTVALGPLRSADARDYDVLIHRKDPPVDAR
                     YRAYAGHFAALPPAGPVQVNQPAEVLRHSEHLLAADFPEYAVPTVHCLDLDALVAAVR
                     AQPLEAVAKPLGECSGHGIVFFAPDAPRAELQGYWDMYGGTGADDPDAGVVVQPYQPA
                     ITTRGDLRILVMNGRVLGSVARLPRPGSRLANLHQGASYSAFDPTPRQLAAVAAVSAR
                     LTDRGLYLLGLDFIGDLLSEINFTSASAMVQINEVMRKRAEVELVDEIERLAAGRRPA
                     Y"
     gene            1733..>2460
                     /locus_tag="VGB85_24130"
     CDS             1733..>2460
                     /locus_tag="VGB85_24130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137202.1"
                     /translation="MTTNTGTTLSRRKLCALLGALPALSSVRCKGQTRDLTLYNWGEY
                     LAPEVVERFAARERGATVSQDFYLSEGELFAKLQASASFDLCVPMDYQLSRLQQHDFI
                     REIDLKKVPGAANLAEKFPPWRAREDRGGGCFAIPYLWGTTGIGYDSDKVEAPTSWRA
                     LFDVRNAGRISVIDSKGDVFDQALLASGLGINSTDKPALQTKIYPMLVDQKRILRAYD
                     SNPARALVSGETWIAQIDSGDLMRA"
BASE COUNT          377 a          790 c          952 g          341 t
ORIGIN      
        1 gctcgtgatc tccgcgtcgt cggtcgaccc gccggcggct tgaccgcagg cgagtggcaa
       61 cgcagcgaga acacaggcgc gagccaaccg tttcatcggc gatcaacgta ggccaaaggg
      121 attggcggtg tcgagcggcc caggagcagg cgcgtcgttg cgctgccccg cggcacagca
      181 tggctcgcgg tcgcccccgc cccgccgagt gtgcgtcccc cgcgtccacc aagctgacct
      241 ttccgccagg tgacgcgaac gcgcgtcggc ggccacgggg cccagaagtg aagcagctgg
      301 cctttccgcc aggtgttttg ccgggcgggg ccggcgccac gaagggcatc agcggccggc
      361 cagcgggctt tgcacgggct ttctcgcgcc tgaccgtagg gacatgtcac tgcgggagga
      421 tgatgccgtg gttgcgtgat ctgatcgagg acaacggcgc gtgcgacgag tatgagacgg
      481 agacacgccg cgagcgtgag ccatcgacga cgagcgcctc gcgggcgtcg ccgcgggccg
      541 cgtgtcgact ctccgatgtg cgcacttgtg agctgtcccg gaggacagct cagtatgcag
      601 ggcggcggcc ggcggcgagg cgctcgatct cgtcgacgag ctcgacctcg gcgcgcttgc
      661 gcatgacctc gttgatctgg accatcgccg aggcgctggt gaagttgatc tccgagagca
      721 ggtcgccgat gaagtcgagg ccgagcaagt agaggccgcg gtcggtcagt cgggcggaca
      781 cggcggcgac ggccgcgagc tggcgcggcg tcgggtcgaa ggcggagtag ctggcgccct
      841 gatgcaggtt ggcgaggcgg gacccgggcc gcgggagccg ggcgacgctg ccgagcacgc
      901 ggccgttcat cacgaggatc cgcaggtcgc cgcgggtggt gatcgccggc tggtagggct
      961 gcacgacgac gccggcgtcg gggtcgtccg cacctgtccc tccgtacatg tcccaatagc
     1021 cctgtagctc ggcgcgcggg gcgtccggcg cgaagaagac gatgccgtgg ccgctgcact
     1081 cgccgagcgg cttggcgacg gcctccagcg gctgcgcccg cacggcggcg acgagcgcgt
     1141 cgaggtcgag gcagtgcacg gtcggcacgg cgtactcggg gaagtcggcg gcgagcaggt
     1201 gctccgagtg ccgcaggacc tcggcgggct ggttgacctg cacgggccct gcgggcggca
     1261 gcgcggcgaa gtggccggcg taggcccggt acctggcgtc gaccggcggg tccttgcggt
     1321 ggatgaggac gtcgtagtcg cgggcgtcgg cgctgcgcag ggggccgagc gcgacggtgg
     1381 ggacgcgggt ggggtcggcg tcgaggacgg cgtgcaccgg caacgcggcg aggtaacgcg
     1441 ggccgggcag cgcggtgtcg gtggcgagca ggtcgcagta gtcgacgacg gcccgacgtg
     1501 cgcgtagctc ggccatcagg cgcagcgtcg tgtcgaagcg cggctgaaag ctggcgatcg
     1561 gatcggcggt gatcaggtac ttgaggggct tcgccatcgc ggaccgttcg gcggaggtgt
     1621 gagcgcccgc cggggccgga ggatgacaaa agcctggccg tgcggaggcg ccgccgcaga
     1681 cgccagaggc gtgatgttgc gctcgcgcca gcgcggagta tacgttccga ggatgacgac
     1741 gaacaccggg acgacgctca gtcggcgaaa gctctgcgcg ctgctgggag ccctcccggc
     1801 gctgtcgagc gtgcgttgca agggccagac gcgcgacctg acgctgtata actggggcga
     1861 atacctcgcg cccgaggtcg tcgaacgctt cgcggcccgc gagcgcggcg cgacggtcag
     1921 ccaggacttc tatctctcgg agggcgagct gttcgccaag ctgcaggcga gcgcgagctt
     1981 cgacctgtgc gtgccgatgg actaccagct gtcgcgcctg caacagcacg acttcatccg
     2041 cgagatcgac ctcaagaagg tgcccggggc ggcgaacctc gcggagaagt ttccgccgtg
     2101 gcgcgcccgc gaggatcgcg gcggcggctg cttcgcgatc ccgtacctgt ggggcacgac
     2161 gggcatcggc tacgacagcg acaaggtcga ggcgccgacg agctggcgcg cgctgttcga
     2221 cgtgcgcaac gccggccgga tcagcgtgat cgacagcaag ggcgatgtct tcgaccaggc
     2281 gctgctcgcc tcgggcctcg ggatcaacag caccgacaag ccggcgctgc agaccaagat
     2341 ctacccgatg ctcgtcgacc agaagcggat cctccgcgcc tacgacagca acccggcgcg
     2401 cgcgctggtc tccggcgaga cgtggatcgc ccagatcgac agcggtgacc tgatgcgcgc
//
LOCUS       DASWNJ010001450         1934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_447925, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001450 DASWNJ010000000
VERSION     DASWNJ010001450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447925"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VGB85_24135"
     CDS             <1..788
                     /locus_tag="VGB85_24135"
                     /inference="COORDINATES: protein motif:HMM:NF020671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HEY0137203.1"
                     /translation="LGRALASPDPALRVRALLRDAGALGSDRDTLERRAGLVDLEPVL
                     SNMRATGEALALGEQRWVDAAVLAPLADAAAAQVDRFQREHPLQPGLGRAAVVGGLPG
                     RVAADVAAAAVAHAVTCGLLLADGDLLKRATTAPDAALPPKVQAVLDLFRRAGIAPPT
                     LREVQDQAGLGERQALEALGALQRSGALVRVSTELSLAREHHEALLERARAHLRSHGR
                     LDVQSLKTLTGLSRKFVVPFLEHLDRLQISRRQGDLRVPGPRL"
     gene            complement(796..>1934)
                     /gene="selA"
                     /locus_tag="VGB85_24140"
     CDS             complement(796..>1934)
                     /gene="selA"
                     /locus_tag="VGB85_24140"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEY0137204.1"
                     /translation="TDLLMPRPRRVLNCTGVLLHTNLGRAPLGDEAVAAMVGAAGSCE
                     LEFAVEEGRRGSRMTWLRPLLRLLLATDDEDVHVVNNGAAALLLACAALGQPGGVVLS
                     RGQMVEIGDGFRVAEMAAASGVRVHEVGSTNRTHLRDYAAALDAGASAILWVHLSNFV
                     QAGFVAQPGLAELRELAEARGVPVIADLGSGAVDGRSREPGAREYLQAGAQVVTFSGD
                     KLLGGPQAGLLIGHAALVERCRRHPLARALRPDKTAIAALHATAAAHARDDGSLPLQR
                     MIGATVEELRARAVAIVAALGWPAECVRASEATIGGGSLPGDVIASVAIAVPGRPEAT
                     ARRLRTGSPAVVGRVHAQTLLLDLRSLLPGDDAALVVALRASCP"
BASE COUNT          243 a          875 c          588 g          228 t
ORIGIN      
        1 ccctcggacg cgccctcgca tcaccggacc cggccctgcg cgtgcgcgcc ctcctgcgcg
       61 acgcgggcgc cctcggcagc gaccgcgaca ccctcgagcg ccgggccgga ctcgtcgacc
      121 tcgaacctgt cctctcgaac atgcgcgcca ccggcgaggc gctcgcgctc ggcgagcaac
      181 gctgggtcga cgccgccgtg ctcgccccgc tcgccgacgc cgccgccgcg caggtcgacc
      241 gcttccagcg cgaacacccc ttgcaacccg gcctcggccg cgcggccgtc gtcggcggcc
      301 tgccgggccg ggtcgccgcg gacgtcgccg ccgccgcggt cgcccacgcc gtcacctgcg
      361 gcctgctgct cgccgacggc gacctgctca aacgcgccac cacggccccc gacgccgccc
      421 tgccgcccaa ggtgcaggcc gtgctcgacc tcttccgccg cgccggcatc gccccgccga
      481 ccctgcgcga ggtccaggac caggccggcc tcggcgagcg ccaggccctc gaggccctcg
      541 gcgccctcca gagatctggc gccctcgtgc gggtctcgac cgagctctcg ctcgcccgcg
      601 agcaccacga ggccctgctc gagcgcgccc gcgcccacct gcgcagccac ggccgcctcg
      661 acgttcaatc tttaaaaacc ctcaccggtt tgtcgcgcaa gttcgtggtc ccgttcctcg
      721 agcacctcga ccgcctgcaa atttcccgcc gtcagggcga cctgcgcgtc cccggcccgc
      781 gcctgtgacc tgtcctcagg gacagctcgc tcgcagcgcc acgaccagcg ccgcgtcatc
      841 cccgggcagc agcgagcgca ggtcgagcag cagcgtctgc gcatgcacgc gcccgaccac
      901 cgcgggcgac cccgtgcgca gccgcctcgc cgtcgcctcc ggccgccccg gcaccgcgat
      961 cgccacgctc gcgatcacat cccccggcag cgacccgccg ccgatcgtcg cctcgctcgc
     1021 gcgcacacac tccgccggcc acccgagcgc cgcgacgatc gccacggccc gcgcccgcag
     1081 ctcctccacc gtcgcgccga tcattcgttg cagcggcaag ctcccgtcgt ctcgcgcgtg
     1141 cgcggccgcc gtcgcgtgca acgccgcgat cgccgtcttg tccggccgca gcgcccgcgc
     1201 cagcggatgc cggcgacagc gctcgaccag cgccgcgtgc ccgatcagca gccccgcctg
     1261 cggcccaccg agcagcttgt caccgctgaa ggtcacgacc tgcgcgcccg cctgcagata
     1321 ctctcgtgcg cccggctccc gcgaccgtcc atcgaccgcc cccgacccga ggtccgcgat
     1381 caccggcacg ccacgcgcct cggcgagctc gcgcagctcc gcgagccccg gctgcgcgac
     1441 gaagcccgcc tgcacgaagt tactcaggtg cacccagagg atcgccgagg ccccggcgtc
     1501 gagcgccgcc gcgtagtcgc gcaggtgcgt gcggttggtg ctgccgacct cgtgcacccg
     1561 caccccgctc gccgccgcca tctcggcgac gcgaaacccg tccccgatct cgaccatctg
     1621 cccgcgtgac agcaccaccc cgcccggctg ccccagcgcc gcacaggcga gcagcagcgc
     1681 cgccgctccg ttgttgacca cgtgcacgtc ctcgtcgtcc gtcgccagca acagccgcag
     1741 cagcggccgc aaccatgtca tccgcgaccc gcgacgcccc tcctcgaccg cgaattccag
     1801 ctcgcagctg cccgcggccc ccaccatcgc cgccaccgcc tcgtcgccga gcggcgcccg
     1861 cccgaggttg gtgtgcagca gcacccccgt gcagttgagc acccgccgcg gccgcggcat
     1921 gagcaagtcc gtca
//
LOCUS       DASWNJ010001451         3170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_165472, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001451 DASWNJ010000000
VERSION     DASWNJ010001451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165472"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VGB85_24145"
     CDS             complement(<1..428)
                     /locus_tag="VGB85_24145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137205.1"
                     /translation="MTIASGLALFGWAALRLFGLLRAQVLWQRALGPVWQGVAAALAI
                     AGALVLWPGAPAEAVPVGRFVVVGVCEFLLGSVLGGLLALPGIALLGAAGQTAATLQA
                     PRGGALPLLLAVACVSGGLMAGVHRPALVASRELLVLWPVG"
     gene            complement(425..691)
                     /locus_tag="VGB85_24150"
     CDS             complement(425..691)
                     /locus_tag="VGB85_24150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137206.1"
                     /translation="MSAPLLEQTLRLAVAQVLPVALAAGGAAVVAGVIAHRLGLHDPT
                     LVLLARAAAVLALLAIGGAAWLADTAAWTGELWSQIAAVGQGRP"
     gene            complement(688..1305)
                     /locus_tag="VGB85_24155"
     CDS             complement(688..1305)
                     /locus_tag="VGB85_24155"
                     /inference="COORDINATES: protein motif:HMM:NF013011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137207.1"
                     /translation="MLPDLPPGIAAWIALTAAPLLAITCTAFTKASVVLSALRVGLGA
                     ETLLPWSAVFALSLAITAVVMGPVAAASLEALEAAGGIENLTRGPGAQIVDVLAPLQQ
                     FLQRHADAGELGFFVELQGVPAEHPLALVPAFLLTELGEALQISLLILVPFVLVDLVV
                     AQVLLLLGIGSGVSQPLVSLPLKISLFLAVGGWDVIVRGLVEGYA"
     gene            complement(1295..1582)
                     /locus_tag="VGB85_24160"
     CDS             complement(1295..1582)
                     /locus_tag="VGB85_24160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEY0137208.1"
                     /translation="MLAELDERLVFALVGLVLLLTLVVLARLTRGGAKAATKVIALGS
                     GHRLHVVELEGRRLLIGTGPSGPPNLVTELADLPAWTHAAVNSDWQGTRAP"
     gene            1720..2325
                     /locus_tag="VGB85_24165"
     CDS             1720..2325
                     /locus_tag="VGB85_24165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137209.1"
                     /translation="MKTWTLIAALALLPGCGDNGGGGADDSSGGTANATTDDTPTGGG
                     EDETSVPPTAGHDAIQAWLMAGHYKSWKCETGVQDPISISPHGKQRICSNPILSAHTE
                     ATEYPVDSAAVKELYDEAGTTIVGYAVYRHIKAGTGGDSWYWYEQLPLDHPAMPDPET
                     GVIADGPGDSGNSLKICVGCHQATGSDAMHPGHDFVYEQIK"
     gene            complement(2418..2891)
                     /gene="rplQ"
                     /locus_tag="VGB85_24170"
     CDS             complement(2418..2891)
                     /gene="rplQ"
                     /locus_tag="VGB85_24170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEY0137210.1"
                     /translation="MRHQNTGRKLSRSSSHRAAMFRNMVTSLIEHERITTTEPKAKEL
                     RRFAERTISISLRLGDLLSKPREERTPAEQGRYIHALRMASRMIRTREVLHKLFDDVA
                     PKLAGRPGGYTRIMKIGQRTGDAAPMAIIELVTAGPPGNKSAAKVVKATGEANIE"
     gene            complement(2895..>3170)
                     /locus_tag="VGB85_24175"
     CDS             complement(2895..>3170)
                     /locus_tag="VGB85_24175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HEY0137211.1"
                     /translation="PQQSETDKLNEYLWRSVDELELSVRSANCLQNANIHYIGDLVQR
                     SEAEMLKTKNFGRKSLKEIKEILAQMGLSLGMKIDNWQGPPPAREET"
BASE COUNT          531 a         1150 c         1091 g          398 t
ORIGIN      
        1 ccgacgggcc acagcaccag cagctcgcgc gacgcgacca acgccgggcg atgcacccct
       61 gccatcaatc cgcccgacac gcaggcgacc gcgagcaaca gcggcagcgc gccgccgcgg
      121 ggcgcctgga gggtggccgc cgtctgtccg gccgcgccga gcagcgcgat cccaggcagc
      181 gccagcaacc caccgagcac gctgccgagc aaaaactcgc agaccccgac cacgacgaag
      241 cgaccgacgg gcaccgcctc ggccggcgcc ccgggccaca gcaccagcgc ccccgcgatc
      301 gccagcgccg cggcgacgcc ttgccacacc ggcccgagag cgcgttgcca cagcacctgc
      361 gcgcgcaaca agccgaacag ccgcagcgcc gcccacccga acagcgcgag cccgctggcg
      421 atcgtcacgg ccggccctgc ccgaccgccg cgatctgcga ccacagctca ccggtccacg
      481 cggcagtgtc ggccagccag gccgcgccgc cgatcgccag caacgcgagc acggccgccg
      541 cgcgggccag cagcaccagc gtcgggtcgt gcaggccgag gcgatgcgcg atgaccccgg
      601 cgaccaccgc cgcgccgccg gccgcgaggg cgaccggaag cacctgcgcg accgccaggc
      661 gcagcgtctg ctcgagcagc ggcgcgctca cgcgtagccc tcgaccagcc cgcgcacgat
      721 gacgtcccac ccaccgaccg ccaggaacag cgagatcttg agcggcaggc tgacgagtgg
      781 ttgcgacacc ccgctaccga tcccgagcag cagcagcacc tgcgcgacga cgaggtcgac
      841 gagcacgaag ggcaccagga tcagcagcga gatctgcagc gcctcgccga gctcggtcag
      901 caggaacgcg ggtaccagcg cgagcgggtg ctcggccggc accccttgca gctcgacgaa
      961 gaagccgagc tcgcccgcgt cggcgtgccg ctgtaagaat tgttgcagcg gcgcgagcac
     1021 gtcgacgatc tgcgcacctg gcccgcgggt caggttctcg atcccgccgg cggcctcgag
     1081 cgcctcgagg ctcgcggccg cgaccgggcc catcaccacc gcggtgatcg ccaggctgag
     1141 cgcgaacacg gcgctccacg gcagcagcgt ctcggccccg aggcccacgc gcagcgccga
     1201 cagcacgacg ctcgccttgg tgaacgccgt acaggtgatc gccagcagcg gcgcggccgt
     1261 cagcgcgatc caggcggcga tgccgggcgg caggtcaggg agcacgcgtt ccctgccagt
     1321 cgctgttcac ggccgcgtgg gtccacgccg gcagatcggc gagctcggtg acgaggttcg
     1381 gcggcccgct cggaccggtc ccgatcagca gacggcggcc ctcgagctcg acgacgtgca
     1441 agcggtgtcc agaccccagc gcgatgacct tcgtcgccgc cttcgcgccg ccccgcgtca
     1501 ggcgggcgag cacgaccagc gtcagcagca ggaccaggcc gaccagcgcg aacacgagtc
     1561 gttcatcgag ctccgcgagc atgggcgaac actggacggt ggacgccggg atcgcaagaa
     1621 atgagccgtc cgacggacgg cgcgtaacac ggcgagccgc tgccgcgttg gatcgcagcg
     1681 acgccttacg ccgcacaacc tgaagactgg agagttccca tgaagacctg gacattgatc
     1741 gctgccctgg cgctcctgcc cggctgcgga gacaacggcg ggggcggtgc ggacgacagc
     1801 tccggcggca cagcgaacgc cacgacggac gacacgccga cgggcggcgg tgaggacgag
     1861 accagcgtgc cgccgaccgc cggccacgac gcaattcagg cgtggctaat ggccggacac
     1921 tacaagagct ggaagtgcga gaccggcgtg caggacccga tctcgatctc cccgcacggc
     1981 aagcagcgga tctgctccaa cccgatcctc agcgcccaca ccgaggcgac cgagtacccg
     2041 gtcgattccg ccgcggtgaa ggagctgtac gacgaggccg gcacgacgat cgtcggctac
     2101 gcggtgtacc gccacatcaa ggccgggacc ggcggcgaca gctggtactg gtacgagcag
     2161 ctgccgctcg accaccccgc gatgcccgac ccggagaccg gcgtgatcgc cgacggcccc
     2221 ggagactccg ggaactccct gaagatctgc gtcggctgcc atcaggcgac cggcagcgat
     2281 gcgatgcacc ccggccacga cttcgtgtac gagcagatca agtaggcgcg cggaccaacg
     2341 gcgaccaaaa caagcgagcc cgcggaggac atcccccgcg ggctcgtttg ctgcttacac
     2401 gcgccggagc gcgcggttta ctcgatgttg gcttcgccgg tggccttgac caccttggcg
     2461 gcgctcttgt tccccggggg gcccgcggtc accagctcga tgatcgccat cggggcggcg
     2521 tcgccggtgc gctgaccgat cttcatgatg cgcgtgtagc cgccgggacg accggcgagc
     2581 ttcggcgcga catcgtcgaa cagcttgtgc aggacctcgc gggtgcggat catgcggctg
     2641 gccatgcgca gggcgtggat gtaccgaccc tgctcggccg gcgtccgctc ctcgcggggc
     2701 ttgctgagca ggtcgccgag gcgcagggag atggagatcg tcctctccgc gaagcggcga
     2761 agctccttgg ccttgggctc ggtggtggtg atgcgctcgt gctcgatgag cgaggtgacc
     2821 atgttgcgga acatggccgc ccggtgggac gagctacggc tgagtttgcg gccggtattc
     2881 tggtggcgca tgggttaggt ctcctcgcgg gccggcggcg gcccctgcca gttgtcgatc
     2941 ttcatgccga gagacaggcc catctgcgcc aggatctctt tgatctcctt gagcgacttc
     3001 cggccgaagt tcttggtctt gagcatctcg gcctcgctgc gctgcacgag gtcgccgatg
     3061 tagtggatgt tcgcgttctg caggcagttc gcggagcgca cgctgagctc gagctcgtcc
     3121 accgagcgcc acaggtactc gttgagcttg tcggtctcgc tctgctgcgg
//
LOCUS       DASWNJ010001452         4284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_408109, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001452 DASWNJ010000000
VERSION     DASWNJ010001452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408109"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VGB85_24180"
     CDS             <1..624
                     /locus_tag="VGB85_24180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137212.1"
                     /translation="DGLPVAREGRVYRRGFISEARVRCAQIYLEPALDHPAWSLLTAL
                     DVSMFTDIEREFLTRAPLTRLRRLSGVHAETLESISLPPTVTHVQPNGRLHHCPEGIE
                     ALEITTYAFGDLWVFGRQEKHPQLALLSIAAHRQPQGEDLAVIRSLLEPPRPARLVLI
                     HIHRAEDEEPVEVWRLEVAGDTWDFRGDDQVLQLLRELTVPVSDEWL"
     gene            742..888
                     /locus_tag="VGB85_24185"
     CDS             742..888
                     /locus_tag="VGB85_24185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137213.1"
                     /translation="MTTTRIADEVHELERAAARFGLLDLLVRGPAYARVTRTYAAFNA
                     APKA"
     gene            1056..2225
                     /locus_tag="VGB85_24190"
     CDS             1056..2225
                     /locus_tag="VGB85_24190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HEY0137214.1"
                     /translation="MSQSDKEHFESYKPQTLAKHTILKNYIAVYFHILKRGEDRIFYI
                     DGFAGRGTYTGDDGSPIPGSPRHALELIASNTELAEKVQTIFIERDKELFSQLKETLR
                     SFHAEHPDVRDPHLENGTFSSKMTEILDSWKKRNIATAPMFVFVDPCGVDGVDFAVIQ
                     RLLSIQRKNEIFLFFNVDGIRRILGLRDSMGDTLARLLGTEDRAAALVRVVDSVRTTS
                     EREEAIIRYYEDLLRANTSGKYVASFRVEKEDRRTTSHYLIHVAQHHRGFSIMKDVMW
                     SVGRTSEGKGGLLFEQASYSRTEALFRPGWDSVKTSILEELRAGPKCVAYFYGELVER
                     PENRLCAAAYRNALLELETEGKILVLNPDGRTPAPKRRTHKGSPTLGERYYVKAR"
     gene            complement(2184..3008)
                     /locus_tag="VGB85_24195"
     CDS             complement(2184..3008)
                     /locus_tag="VGB85_24195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp37/Gp68 family protein"
                     /protein_id="HEY0137215.1"
                     /translation="MSTISSIEWTETTWNPVRGCSRISPGCERCYAERMAHRFSAPGA
                     PYEGLTTLSKKGPRWTGRVQLVHEALHEPITWRRPRLVFVNSMSDLFHDSVPLEFISS
                     VFETMRRAHWHTFQLLTKRAERLAQVAAALPWPENVWMGVSVESRAYQPRIDLLRQVP
                     AHVRFLSLEPLLESLPNLELTGIDWVIVGGESGPGARPIEKCWVHDIFMQCRAASVPF
                     FFKQWGGVRKKEAGRLLDGRTYDEMPVARAAEIRRASRSVHRDSPCLDVVALAQRR"
     gene            complement(3357..>4284)
                     /locus_tag="VGB85_24200"
     CDS             complement(3357..>4284)
                     /locus_tag="VGB85_24200"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0137216.1"
                     /translation="HPAVVLLTGSGAQDRDESILGHKPFLVLADHLTRNGVAVLRYDD
                     RGVGGSGGHLEKTPYATQQKDASAALTWLQGQAEVDPKRIGLVGHSEGANLAVLTAAA
                     DKQVGFIVLLAGMGVSGKEILPMQMAALLRAAGAAEAALPGLVALENKLIAAILKRAP
                     RAEIEGLARDLTRGQLDQSKPPTAVTPEQLEVLVQSSLAAFETESFLSLLRHDPRPAL
                     RKLKKTPVLALGGSLDLQVPPAENLGEIEKALKAARNKDFTLKTLPGLNHLFQHASTG
                     QVEEYGKIEETFAPEALQEISAWILAHTSPPK"
BASE COUNT          749 a         1405 c         1397 g          733 t
ORIGIN      
        1 gacggactac ccgtcgcgcg cgagggccgg gtctaccgcc gcggcttcat ctccgaggcg
       61 cgcgttcgct gcgcgcagat ctacctcgag cccgcgctcg accacccggc gtggagcctg
      121 ctgaccgcgc tcgatgtcag catgttcacg gatatcgagc gcgagttcct gacgcgcgcg
      181 ccgctcactc gcctgcggcg tctctccggc gtccacgccg agacgctcga gtcgatctcg
      241 ctgccgccga cggtcaccca cgtgcagccg aacggccgcc tccatcactg ccccgagggg
      301 atcgaagcgc tggagatcac gacctacgcc ttcggcgacc tgtgggtgtt cggccgccag
      361 gagaagcacc cgcagctcgc gctgctctcg atcgcggcgc accgccagcc gcagggcgag
      421 gacctcgcgg tgatccgcag cctgctcgag ccgccccggc ccgcgcgcct ggtgctgatc
      481 cacatccacc gcgcggagga cgaggagccc gtcgaggtgt ggcgcctcga ggtcgccggc
      541 gacacctggg acttccgcgg cgacgaccag gtgctgcagc tgctgcgcga gctgacggtc
      601 ccggtgtccg acgagtggct ctgagcggcc gctccctcgc caggcgcacg atgctcgcct
      661 cgccacgctc tcgaggcgca gcgcgctcgc cccgagacgt cgggcccgcc cctccgcgcc
      721 ggcctccggc gtgcgtgcac gatgaccacc acgcgcatcg cggacgaagt tcacgagctc
      781 gagcgcgcag cagcgcgctt cggcctgctc gacctcctag tccgcggccc ggcgtacgcg
      841 cgcgtcaccc ggacctacgc ggccttcaac gccgccccca aggcctgagc tcctggcatc
      901 gcaggatcca cgttctccgg ttgtatagtc gacatcccag ggatcctcgc cactcgtgcg
      961 cgccggcttc tcgacggcaa acagaccacc aaaaagaatt ttggttgcgc cagccatcag
     1021 cgtcgcggaa cctgtccgct gaggaggagt cgaggatgtc gcagtcggac aaggagcact
     1081 tcgagagcta taagccccag acgctggcca agcacaccat cttaaagaac tatatcgccg
     1141 tctacttcca tatcctgaag cgtggggagg accgcatctt ctatatcgac ggcttcgcgg
     1201 ggcggggcac gtacacggga gacgacggga gcccgattcc aggctcgcct cggcacgctc
     1261 tcgagttaat cgcctcgaac accgaactcg ctgaaaaggt ccaaaccatc ttcatcgagc
     1321 gggacaagga acttttctct cagctcaaag agacattgag gtcgttccac gccgagcacc
     1381 ccgacgtccg agatccacac ctggaaaacg ggacattttc ctccaagatg acggagatac
     1441 tcgactcgtg gaagaagcgc aacatagcga cggcgccgat gttcgtcttc gtggatcctt
     1501 gcggcgtgga tggggtcgac ttcgccgtga ttcagcggct gctgagcatc cagcgaaaga
     1561 acgagatctt cctcttcttc aacgtcgacg gtatccgccg catccttggg ttgcgagaca
     1621 gcatgggaga tacactcgcg cgcctcctcg gtaccgagga ccgtgccgcg gcgctcgtca
     1681 gggtcgtcga ctccgtacgg acgacgagcg aacgagagga ggcgattatt cgctattacg
     1741 aggatctgct gcgcgcgaat acctctggga agtacgtcgc gtcgttccgt gtcgaaaaag
     1801 aggatcgccg aacgacaagc cattatttga tccatgtcgc gcaacaccac cgcgggtttt
     1861 caatcatgaa ggacgtaatg tggtctgtcg gcaggacttc cgagggcaag ggcggactgc
     1921 tgttcgagca ggccagctac tcaaggactg aagcgctgtt caggcccgga tgggacagcg
     1981 tgaagacttc aatcctcgaa gagctccgcg cgggacccaa atgtgttgcc tacttctacg
     2041 gggagctcgt cgagagacca gagaatcgtc tttgcgccgc ggcatatcgc aatgctctcc
     2101 tcgaactcga aacggaaggc aaaatcctcg tgctcaaccc agacggacgg actccggcgc
     2161 caaagaggcg tactcacaaa gggtcaccga cgctgggcga gcgctactac gtcaaggcac
     2221 ggtgagtccc gatgcacaga ccgagatgcc cggcggattt cggccgcccg agcgacgggc
     2281 atctcgtcgt aggttcgccc atcgaggagc cgaccggctt ccttcttgcg tacgcctccc
     2341 cactgcttga aaaagaaggg cacgcttgca gcccggcatt gcataaagat gtcatgcacc
     2401 cagcatttct cgatgggcct ggcgcccggc ccggactcgc cgcccacgat aacccagtca
     2461 atgcctgtca attcgagatt cggcaggctc tccagaagcg gttcgaggga caggaaccgg
     2521 acatgggcgg gtacctgtcg cagcaggtcg atccttggct gatacgcgcg ggactcgacg
     2581 ctgaccccca tccagacgtt ctcgggccag gggagagcgg ctgcaacctg cgcgaggcgc
     2641 tcggcccgct tcgtcagtaa ctggaacgta tgccagtggg cgcgacgcat cgtctcgaaa
     2701 accgaggaga taaactccag agggacgctg tcgtggaaca gatcgctcat cgagttcacg
     2761 aacacgaggc ggggacggcg ccacgttatc ggctcatgaa gcgcctcgtg gaccaactgc
     2821 acccgccccg tccagcgcgg ccccttcttg cttagtgttg tcagtccctc atagggggcg
     2881 ccgggcgcgc taaaccgatg ggccatccgc tcggcatagc aacgctcgca gccgggcgat
     2941 atgcgcgagc agccgcgaac cgggttccag gtcgtctcgg tccactcgat ggaagagatc
     3001 gtgctcatcg ccttgcctcc tgccgaccgt gcgtacgaag tggatccgtg gatcgccgcg
     3061 gcagagacgg actgtacatc cgctcatggt cgccgacaag gcgtaacatg cgccgtccgc
     3121 cgcgccatct aaaggccgcg atggcccgca gaggcctcgc cctgatcccc gacctcacgt
     3181 gtccgtgctg tcgctcatgc aggccacctc gtcgggtcat aggcgcagcc ggccagccgt
     3241 cgcacctgta tttcagcggt aggtcgtgcc atctgtgcac atacttgtga tctcgaaacg
     3301 gcgttcgtgt gaaagtgtct cacgaacgcc ggtctcatcg ccgcacttga atggtctcac
     3361 ttcggcgggc tggtatgcgc gaggatccac gcgctgatct cttgcagggc ctcgggggcg
     3421 aaggtctcct cgatcttgcc gtactcctcg acctggccgg tgctcgcgtg ctgaaacagg
     3481 tgattgaggc cggggagggt cttgagggtg aagtccttgt tgcgggcggc cttgagggcc
     3541 ttctcaattt cgccgaggtt ctcggccggg gggacctgga ggtcgaggga gccgccgagg
     3601 gcgagcacgg gggtcttctt gagcttgcgc agcgcggggc gcgggtcgtg gcgcagcagg
     3661 gacaaaaacg actcggtctc gaaggcggcg aggctggact ggacgagcac ctcgagctgc
     3721 tccggggtga ccgcggtcgg tggtttgctc tggtcgagct ggccgcgggt gaggtcgcgg
     3781 gcgaggcctt cgatctcggc gcgtggggcc cgcttcagga tcgcggcgat cagcttgttc
     3841 tcgagggcga cgaggcccgg gagcgcggcc tcggcggcgc ccgcagcacg cagcagcgcg
     3901 gccatctgca tcggaaggat ctccttgccg gagacgccca tgccggccag caggacgatg
     3961 aagccgacct gcttgtcggc ggcggcggtg aggaccgcca ggttggcgcc ctcgctgtgg
     4021 ccgacgaggc cgatgcgctt cgggtcgacc tcggcctgtc cttgcagcca ggtcagcgcg
     4081 gcgctggcgt ccttttgctg ggtcgcgtag ggcgtcttct cgaggtggcc gccggagccg
     4141 ccgacgccgc ggtcgtcgta gcgcagcacg gcgacgccgt tgcgcgtcag gtggtcggcg
     4201 agcacgagga agggcttgtg gcccaggatc gactcgtcgc ggtcctgcgc gcccgaaccg
     4261 gtcagcagca cgacggccgg gtgc
//
LOCUS       DASWNJ010001453         1695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_202923, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001453 DASWNJ010000000
VERSION     DASWNJ010001453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202923"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1423)
                     /locus_tag="VGB85_24205"
     CDS             complement(<1..1423)
                     /locus_tag="VGB85_24205"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0137217.1"
                     /translation="MTQDPVAGTIDDHGPELLEGEEAPPPGVRAMALVRWLLLAAIAV
                     VAGAAWWSYAHADLSHAHAPRFQCPMHPQITAPDPGECPICHMALEPIDRARTEQPPP
                     ASDAPTVYRCPMHDEQQALSPGTCAICKMDLGPLPPEQAPIVYECPMHAEQRSLSPGT
                     CEICKMDLEPLARVIPPPGTAPVDITLDRRQAIGVRTAEVRAMRHTPVLRAPASLQLA
                     ESGAAQVHVRAAGFLEKVVVAETGVRVHKGQRLAELYSPEIYQAQAELLAARRWSGSE
                     AGNALGAARQRLELLGMSSAAIDRVITSGKPARTVAVEAPAGGVIVARSAVLGAYVGP
                     ETALYEIRDRDTLYVIAEVPAGRGEAITAGTKAVLKIPTRPEFRRDLEVDLVYPELDR
                     EARSFRARMTITDPDLRAGEYAIVEFALPARDVLAVPRDAVIGTGGGDHVFVDRGEGR
                     LEPRVVTVGLCWDELCDLTAGVAA"
     gene            complement(1420..>1695)
                     /locus_tag="VGB85_24210"
     CDS             complement(1420..>1695)
                     /locus_tag="VGB85_24210"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY0137218.1"
                     /translation="TAARRWQTLRGAALPASVRAREVALSGYEVGRADMLGMLAAEGA
                     YVEISLEIIDAKAALDHALAELERAGGDALPRTPLLPTDPHTHRGAR"
BASE COUNT          203 a          562 c          700 g          230 t
ORIGIN      
        1 ccgcggcgac gcccgcggtc aggtcgcaca gctcgtccca gcacaggccg acggtgacga
       61 cgcgtggttc gagtcggccc tcgccgcgat cgacgaacac atgatcgccg ccgccggtgc
      121 cgatcacggc gtcgcgcggg acggcgagca cgtcgcgcgc gggcagggca aactcgacga
      181 tcgcgtactc gccggcgcgc aggtcggggt cggtgatcgt catgcgcgcg cggaagctgc
      241 gggcctcgcg gtcgagctcg gggtagacga ggtcgacctc gaggtcgcga cgaaactcgg
      301 ggcgggtcgg gatcttcagg acggccttcg tgccggcggt gatcgcctcg ccgcggccgg
      361 cggggacctc ggcgatcacg tagagggtgt cgcggtcgcg gatctcatac aaagcggtct
      421 cggggccgac gtaggcgccg agcacggcgc tgcgggcgac gatgacgccg ccggccgggg
      481 cctcgacggc gacggtgcgc gccggcttgc cgctggtgat cacgcggtcg atcgcggcgc
      541 tgctcatgcc gagcagctcg agccgctggc gcgccgcgcc gagcgcgttg ccggcctcgc
      601 tgccggacca gcgccgcgcg gcgagcagct cggcctgggc ctgatagatc tccgggctgt
      661 agagctcggc gaggcgctgg cccttgtgca cgcgcacgcc ggtctcggcg acgacgactt
      721 tttccaggaa gcccgcggcg cgcacgtgga cctgggcggc gccggactcg gccagctgca
      781 ggctcgccgg ggcgcgcaat acgggggtgt gtcgcatggc gcggacctcc gcggtgcgca
      841 caccgatcgc ctgccggcgg tcgagggtga tgtcgaccgg cgcggtgccc ggcggcggga
      901 tcacgcgggc gagcggctcg aggtccattt tacaaatttc acatgtcccc ggggacaggg
      961 agcgctgctc ggcgtgcatc gggcattcgt agacgatcgg ggcctgctct ggggggagcg
     1021 gcccgaggtc catcttgcaa atggcacatg tcccggggga cagggcctgc tgctcgtcgt
     1081 gcatcgggca gcggtagacg gtcggggcgt cgctggcggg cggtggttgc tccgtgcgcg
     1141 cgcggtcgat cggctcgagg gccatgtggc agatcgggca ctcgccgggg tcgggcgcgg
     1201 tgatctgcgg gtgcatcggg cactggaagc gcggggcgtg ggcgtggctg aggtcggcgt
     1261 gcgcgtagga ccaccaggcg gcgcccgcga cgacggcgat cgcggcgagc agcagccagc
     1321 ggaccagggc catcgcgcgc acgcccggcg gcggggcctc ctcgccctcg agcagctcgg
     1381 ggccgtgatc gtcgatcgtg ccggcgacgg ggtcctgggt catcgtgcgc ctcggtgggt
     1441 gtgcgggtcg gtcggcagca gcggggtgcg cgggagggcg tcgccgccgg cgcgctcgag
     1501 ctcggcgagg gcgtggtcga gggcggcctt ggcgtcgatg atctcgagcg agatctcgac
     1561 gtaggcgccc tcggccgcga gcatgccgag catgtcggcg cggccgacct cgtagccgga
     1621 cagcgcgacc tcgcgggcgc gcacgctcgc cggcagggcc gcgccgcgca gggtctgcca
     1681 gcggcgcgcg gcggt
//
LOCUS       DASWNJ010001454         2853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_591339, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001454 DASWNJ010000000
VERSION     DASWNJ010001454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591339"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VGB85_24215"
     CDS             complement(<1..889)
                     /locus_tag="VGB85_24215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137219.1"
                     /translation="MTAPSHDTHAGLLDGEDHRFRVELRGSAEPDGPYPRGTFRQIVT
                     AMITLVTLIGLTYAVPRWHWARPWTADMDYVPFWNLIGRVLLGEGEAVAKVDAELAEA
                     QALARAELEIEDKAEPFVDREVRKPAPTGPELEKLPALAPSPEDSERVERTLENAETL
                     DPFYAALAASELGYAGAITRVMHYGDSAIGNDGIPGAIRLKMQARFGDAGHGFHLLGQ
                     PNASYKHQGVRFEEKSPWNHCFIIFGCREKDGHYGLGGTTFESVGGAEVSLGTASKGP
                     MGRKVSRFELWYAGMPKGGN"
     gene            complement(894..2552)
                     /locus_tag="VGB85_24220"
     CDS             complement(894..2552)
                     /locus_tag="VGB85_24220"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEY0137220.1"
                     /translation="MLFVSFDFLLFIIPVLLLSWALANVPVLRVLFLIAASYFFYMAG
                     PHTDPIQPPWYFVFLLLASTVLDYVCSHRIAARRPEMDSADPSTQARARRARNAWLGV
                     SLVGNLGLLGYFKYTNFFLGAFADIAAALGVEVVVPHLRLILPIGISFYTFQTLSYTI
                     DVWRGRLTPEPNFRRFALFVVFFPQLVAGPIVRAHEFLPQLHRPPQLSASNMSQGMYR
                     ILCGVLKKAILGDWIAAQFTDAVFAAPDTYTSLEILLALYAFTLQLYADFSGYSDIAI
                     GVALLLGYTLPENFDRPYQATNLGEFWRRWHMTLSTWLRDYVFFPLGGSRGSPARVYF
                     NLWLTMFLVGMWHGASWNFIIYSNVHALAMVFNRWNRGRQQAVHGRFTLARSAPWVVG
                     ILLLSAAVFGLGVNVLRLDPGTSAGLAGFAAAMFVIVAALPETGKWWNVALHVLLTFH
                     FTVISRVFFRADDLDTSRAMIAGMLRLDTHGIREGLLSPGAWAVLVGGLAYHMTPKRW
                     VEQYGYEVFRRLPGPVIGLLFAAASLGVYLLLSGGPRANIYFQF"
BASE COUNT          478 a          957 c         1026 g          392 t
ORIGIN      
        1 tgttgccgcc cttcggcatg cccgcgtacc acagctcgaa gcgcgagacc ttgcgcccca
       61 tcgggccctt gctcgccgtg ccgagcgaga cctcggcgcc gccgaccgac tcgaaggtcg
      121 tgccgccgag gccgtagtgg ccgtccttct cgcggcagcc gaagatgata aagcagtggt
      181 tccacggcga cttctcctcg aagcgcacgc cctgatgctt gtagctggcg ttgggctgcc
      241 cgagcaggtg aaagccgtgg ccggcgtcgc cgaagcgggc ctgcatcttg aggcggatcg
      301 cgccggggat gccgtcgttg ccgatcgcag agtcgccgta atgcatcacc cgcgtgatcg
      361 cgccggcgta gccgagctcg ctcgccgcca gcgcggcgta gaaggggtcg agggtctcgg
      421 cgttctcgag cgtacgctcg acgcgctcac tgtcctcggg gctcggcgcg agcgcgggca
      481 gcttctcgag ctcggggccg gtcggcgccg gcttgcggac ctcgcggtcg acgaagggct
      541 ccgccttgtc ctcgatctcg agctcggcgc gcgcgagcgc ctgggcctcg gcgagctcgg
      601 cgtcgacctt ggcgaccgcc tccccctcac cgagcagcac gcggccgatc aggttccaga
      661 acggcacgta gtccatgtcg gccgtccacg gccgggccca gtgccagcgc ggcaccgcgt
      721 aggtcagccc gatcaacgtc accagggtga tcatcgccgt gacgatctgg cggaaggtgc
      781 cacgcgggta cgggccgtcg ggctcggccg agccgcgaag ttccacgcga aagcggtggt
      841 cctctccgtc gaggaggccg gcgtgcgtgt cgtgggacgg ggcggtcatg gttctagaac
      901 tggaagtaga tgttggcccg cggtccgccc gagagcagga ggtacacgcc gagcgacgcg
      961 gcggcgaaca gcagcccgat cacgggcccg ggcagccggc ggaacacttc atacccgtac
     1021 tgctcgaccc agcgcttggg ggtcatgtga tacgcgagcc cgccgacgag caccgcccac
     1081 gcgccgggcg acagcaggcc ctcgcggatc ccatgggtgt cgaggcgcag catgccggcg
     1141 atcatcgcgc ggctggtgtc gaggtcgtcg gcgcggaaga acacgcgcga gatcaccgtg
     1201 aagtggaagg tcagcagcac gtgcagcgcg acgttccacc acttgccggt ctcgggcagc
     1261 gccgcgacga tgacgaacat cgccgccgcg aagccggcga gcccggccga ggtgcccgga
     1321 tcgaggcgga gtacattgac gccgaggccg aacaccgccg ccgagagcag caggatgccg
     1381 acgacccacg gcgcgctgcg ggccagcgtg aagcgcccat gaacggcctg ttgtcgcccg
     1441 cggttccagc ggttgaacac catcgccagc gcgtggacgt tgctgtagat gatgaaattc
     1501 cagctcgcgc cgtgccacat gccgaccagg aacatggtca gccacaggtt gaagtacacg
     1561 cgggcgggcg agccgcggct gccgccgagc gggaagaaca cgtagtcgcg cagccaggtc
     1621 gacagggtca tgtgccagcg gcgccagaac tcgccgaggt tggtcgcctg gtacggccgg
     1681 tcgaagttct cgggcagcgt gtagccgagc agcagcgcga ccccgatcgc gatgtccgag
     1741 tagccgctga agtcggcgta gagctggagc gtgaaggcgt agagcgcgag caggatctcg
     1801 agcgaggtgt acgtgtcggg cgcggcgaac accgcgtcgg tgaactgcgc ggcgatccag
     1861 tcgccgagga tcgccttttt gaggaccccg cagaggatgc gatacatccc ctggctcatg
     1921 ttgctggcgc tcagctgcgg cgggcggtgc agctgcggca agaactcgtg cgcgcgcacg
     1981 atcggcccgg cgaccagctg cgggaagaac acgacgaaca gcgcgaagcg gcgaaaattc
     2041 ggctcgggcg tcaggcgccc gcgccacacg tcgatcgtgt agctcagcgt ctggaacgtg
     2101 tagaagctga tcccgatcgg caagatcagc cgcaggtgcg gcaccacgac ctcgaccccg
     2161 agcgccgcgg cgatgtccgc gaaggccccg aggaagaagt tggtatattt aaaatagccg
     2221 agcagcccga ggttgccgac gaggctgacg ccgagccagg cgttgcgggc ccgccgggcc
     2281 cgcgcctgcg tcgaagggtc ggcgctgtcc atctcgggcc ggcgcgcggc gatccggtgc
     2341 gagcagacgt agtcgagcac cgtcgaggcg agcagcagga agacgaagta ccagggcggc
     2401 tggatcgggt ccgtgtgcgg acccgccatg taaaagaagt agctcgccgc gatcaggaac
     2461 agcacccgca gcacgggcac gttcgccagc gcccacgaca gcaggagcac cggaatgatg
     2521 aacagcagga agtcgaagct gacgaagagc atcgcgcagg ggccgtgggc ggccggcccg
     2581 gaagctaccc acgcacgccc ccgaacgcga atcttcggcg caaaactccc gtccggcgcc
     2641 gccggggctg gccggccacc gggcacggcc gtacaatgcg gcatgcgttc tggccgcctc
     2701 gtgtcctgtc agccccgtgg cgcccgcccg gtcgccgtcc tgttggccgc cgcgctcgcc
     2761 tcgaccccgg tcgccgcgca ggagccggtg ccggtccggg tcgagagcca gccggtcgtc
     2821 gtcgagacct tgccggttcc ggccgacgtg tct
//
LOCUS       DASWNJ010001455         1683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_338021, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001455 DASWNJ010000000
VERSION     DASWNJ010001455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338021"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1683)
                     /locus_tag="VGB85_24225"
     CDS             complement(<1..>1683)
                     /locus_tag="VGB85_24225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137221.1"
                     /translation="DRMTGLLHLEALLAVLALWSLGRRLWPRGPWAILAAALYVLSPL
                     AVWVHRTPLTEPLTGLLLLATALALARGLRTGEDMTIVAALLLGMTAWVRGNAWLSAP
                     VVLAILWFAPGTRRRLLAPGLLFALLVASLALHVVTCYPYLYDELHRQLGRLAPLSLP
                     AIAVASVLAAAAWLLGDWLLRPLRGRVAPLPGVLVVAAALAILGYAALAMGGAGHSRL
                     DPAGPLLGAGLLLAAIAGALRVAALRAPADVHAVWLLALAGIPVVTLALYAQRNLPQA
                     TLYYYGRYLHPELLPLACLLAAYALQGLYKILSRRTGPKITALITAGLAIILIVGQAW
                     PHVAYPVTRLQEFAGAERAVDALAAEIPEDAIVIAGGEGWHSAHTFNQIGGALALGRG
                     RSVLPYRSQEAAYATLHELLIRSPAARERPPPRVFLLLNEASHVYRPRDEHGVAGDPV
                     AAIDDRLPAPFVARPVGLVELLVDRLTPVTGVLPTRVTRADLRMGLFEVEVDEALRAA
                     IRAWTFADGAPRGPDGLRLRGASWQAGSLCLDPKQPLTIDLPAGLPGHVSLV"
BASE COUNT          265 a          584 c          670 g          164 t
ORIGIN      
        1 ggaccaaaga gacatgtccc gggagaccgg cgggcaggtc gatcgtcagc ggctgcttcg
       61 ggtcgaggca caggctgccg gcctgccagc tcgcgccgcg caggcgcagg ccgtcggggc
      121 cgcgcggggc cccgtcggcg aaggtccagg cgcggatcgc cgcgcgcagg gcctcgtcga
      181 cctcgacctc gaacagcccc atgcgcaggt cggcgcgcgt gacccgagtc ggcagcacgc
      241 cggtgaccgg ggtcaggcgg tcgacgagca gctcgacgag gccgaccggg cgcgcgacga
      301 agggggcggg caggcggtcg tcgatcgcgg cgaccgggtc gccggcgacg ccgtgctcgt
      361 cgcgcggccg gtacacgtgg ctcgcctcgt tgagcagcag gaacacgcgc ggcggcgggc
      421 gctcgcgggc ggcgggcgaa cggatcagca gctcgtgcag ggtcgcgtag gcggcctcct
      481 gcgagcggta gggcaggacg ctgcggccgc ggccgagcgc gagggcgccg ccgatctggt
      541 taaaagtgtg ggcgctgtgc cagccctcgc cgccggcgat cacgatcgcg tcctcgggga
      601 tctccgcggc gagcgcgtcg acggcccgct cggcgcccgc gaattcttgc agacgcgtga
      661 ccggatacgc gacgtgcggc caggcctgcc cgacgatcag gatgatggcg aggccggcag
      721 tgatgagagc cgtgatcttc ggacctgtcc ttcgggacag gattttataa agtccctgca
      781 gcgcgtacgc ggcgagcagg caggcgagcg gcagcagctc cgggtggagg tagcggccgt
      841 aatagtagag ggtcgcctgc gggaggttgc gctgggcgta gagggcgagg gtgacgaccg
      901 ggatgccggc gagcgcgagc agccacaccg cgtgcacgtc ggccggcgcg cgcagggccg
      961 cgacgcgcag ggccccggcg atcgcggcga gcaacaggcc ggcgccgagc agcgggccgg
     1021 cggggtcgag ccggctgtga cccgcgccgc ccatcgccag cgcggcgtac ccgaggatcg
     1081 ccagcgccgc ggcgacgacg agcacgcccg gcagcggcgc gacgcgtcct cgcagcgggc
     1141 gcagcagcca gtcgccgagc aaccacgccg ccgcagcgag caccgaagcg acggcgatcg
     1201 ccggcaacga cagcggcgcg aggcgaccga gctggcggtg cagctcgtcg tacagatacg
     1261 ggtaacaggt gacgacgtgc agcgcgaggc tggcgaccag caacgcgaac agcaggcccg
     1321 gcgcgagcag cctgcgccgg gtccccggcg cgaaccacag gatcgccagc acgaccggcg
     1381 cgctcagcca cgcgttgccg cggacccacg cggtcatgcc gagcagcagc gcggcgacga
     1441 tcgtcatgtc ctcgccggtg cgcaggcccc tggcgagcgc gagcgcggtc gccagcagca
     1501 gcaggccggt cagcggttcg gtcagggggg tgcgatgcac ccacactgcc agcggcgaaa
     1561 gcacatacag cgccgccgcg aggatcgccc acgggccacg cggccacagg cgccggccga
     1621 gcgaccacaa cgcgagcacg gcgagcagcg cctcgaggtg cagcaggccg gtcatgcggt
     1681 ccg
//
LOCUS       DASWNJ010001456         1958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_237574, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001456 DASWNJ010000000
VERSION     DASWNJ010001456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237574"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VGB85_24230"
     CDS             <1..708
                     /locus_tag="VGB85_24230"
                     /inference="COORDINATES: protein motif:HMM:NF016879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Coq4 family protein"
                     /protein_id="HEY0137222.1"
                     /translation="SRLVSLSGMTIKRYDFGRAARAMRVLERDGPDIDQVFEIADALP
                     GHALVIARARLRLSEVGRRLLRERPNMAAILEDRAPLAAMPGDSVAAAYLRFMSEERI
                     DARSLVDADRRVRRDTAISDEQFVWEHLRDTHDLWHAVTGYHGDLVGEPALQAFMFAQ
                     LGMPGSAFLALAVFAQAPPGVRPVLAEAFFAGMRARWLLGQDWEALLPRPLAEVREQL
                     RVSPAPAYVPMRLSRAA"
     gene            complement(724..>1958)
                     /locus_tag="VGB85_24235"
     CDS             complement(724..>1958)
                     /locus_tag="VGB85_24235"
                     /inference="COORDINATES: protein motif:HMM:NF000658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEY0137223.1"
                     /translation="FVPQLGDGRAVLLGEVVDRHGRRRDVQLKGGGRTPFSRGGDGRA
                     ALGPVLREYLVSEAMAALGVPTTRALAAVTTGEKVRREEMLPGAVFVRVAASHLRVGT
                     FELLAARGDHEGVKVMTSYALRRHYPEAAGAANEALALLEAVTAAQARLIARWLGIGF
                     IHGVMNTDNTSISGETIDYGPCAFLDEYSPGKTFSSIDHAGRYAFAAQPRIALWNLAR
                     LAETLVPAIDEDGERAVALATERLDGFAPLFNEAHGKIQRAKVGLTLTEPEDAALVAD
                     LLARMAANEVDFTLLFRGLCDAVADSARDADVAALFADPDAFHGWAKQWRARLAREPG
                     TPEERRAAMRLVNPAFIPRNHRIEEIIRAAIDRNDYTPFETFNKVLERPFDDQPEFAS
                     WREPPRREQRVCATFCGT"
BASE COUNT          257 a          707 c          717 g          277 t
ORIGIN      
        1 tcccggctcg tgtcgctatc cggcatgacg atcaaacgct acgactttgg acgcgcggcg
       61 cgggcgatgc gggtgctcga gcgcgatggg cccgacatcg atcaggtgtt cgagatcgcc
      121 gacgcgctgc cgggccacgc cctcgtcatc gcgcgggcgc ggcttcgtct gtcggaggtc
      181 ggccgaagac tactgcgcga gcggccgaac atggccgcga tcctcgagga cagggccccc
      241 ctggccgcga tgccgggcga cagcgtcgcg gccgcgtacc tgcgcttcat gagcgaggag
      301 cgcatcgacg cccgctcgct cgtcgacgcc gaccgcaggg tccgccgcga cacggcgatc
      361 tccgacgagc agttcgtctg ggagcacctg cgtgacaccc acgacctgtg gcacgccgtc
      421 accggctatc acggtgacct cgtcggtgag ccggccctgc aggccttcat gttcgcgcag
      481 ctcggcatgc cgggcagcgc cttcctggca ctcgctgtgt tcgcccaggc gccccccggg
      541 gtccgtcccg tgctcgcgga ggcgttcttc gccggcatgc gcgcgcgctg gctgctcggg
      601 caggactggg aggcgctgct gccccggccg ctcgccgagg tccgcgagca gctgcgcgtc
      661 agcccggcgc cggcctacgt gccgatgcgc ctctcacggg ccgcctgatc cggcccccgc
      721 ggatcaggtc ccgcagaagg tcgcgcacac gcgctgctca cggcgcggcg gctcgcgcca
      781 cgacgcaaac tccggctgat cgtcgaacgg ccgctcgagc actttattga acgtttcgaa
      841 gggcgtgtag tcattgcgat cgatcgcggc gcggatgatc tcctcgatgc gatggttgcg
      901 ggggataaac gcggggttga ccagccgcat cgccgcgcgg cgctcctcgg gggtgccggg
      961 ctcgcgggcc aggcgggcgc gccactgctt cgcccagccg tgaaacgcgt cggggtcggc
     1021 gaacagcgcg gcgacgtcgg cgtcacgcgc ggagtcggcg accgcgtcgc acagcccgcg
     1081 gaacagcagg gtgaagtcga cctcgttggc ggccatacgc gcgagcaggt cggcgaccag
     1141 cgcggcgtcc tcgggctcgg tgagcgtcag gccgaccttg gcgcgctgga tcttgccgtg
     1201 cgcctcgttg aacagcgggg cgaagccgtc gaggcgctcg gtggcgagcg cgaccgcccg
     1261 ctcgccgtcc tcgtcgatcg cggggaccag cgtctcggcg agccgggcga ggttccacag
     1321 ggcgatccgc ggctgggcgg cgaaggcgta gcggccggcg tggtcgatcg agctgaaggt
     1381 cttgccgggc gagtactcgt cgaggaaggc gcacggaccg tagtcgatcg tctcgccgga
     1441 gatcgaggtg ttgtcggtgt tcatcacgcc gtggataaag ccgatgccga gccagcgagc
     1501 gatcaaccgc gcctgcgccg cggtgaccgc ctcgagcagc gcgagcgcct cgttcgccgc
     1561 gccggccgcc tcggggtagt ggcggcgcag cgcgtagctc gtcatgacct tgacgccctc
     1621 gtggtcgccg cgcgcggcga gcagctcgaa ggtgccgacg cgcaggtggc tcgccgcgac
     1681 gcgcacgaac acggcgcccg gcaacatctc ctcgcgccgg accttctcgc cggtcgtgac
     1741 ggccgccagc gcgcgcgtcg tcggcacgcc gagcgccgcc atcgcctcgc tgacgaggta
     1801 ttcgcgcagc accggtccca gcgccgcgcg cccgtcgcca ccgcgggaga agggcgtccg
     1861 gccaccgccc ttcagctgca cgtcgcgccg ccggccgtgc cggtcgacga cctcgccgag
     1921 caggaccgcg cgcccgtcgc cgagctgggg gacgaaat
//
LOCUS       DASWNJ010001457         1916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_62057, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001457 DASWNJ010000000
VERSION     DASWNJ010001457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62057"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VGB85_24240"
     CDS             complement(<1..792)
                     /locus_tag="VGB85_24240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137224.1"
                     /translation="MQRLRELVPTVAEEDDEALRDRLDAAITTAEDGGKAVRTLDVML
                     AEPQILIPLESFGETLYAIIKEDTVVTVLPRGHGEEILQRGQALEQRVAAGDPPPRAG
                     ERDEAWDLGRRRWRRDLPTPVVERVRSPGAAGGDDLDDPARPPEFALTSPGTPSPAAG
                     PVAGPASTPAEEPATDEVPTTPLGAGVAARLRSRVGGNRLIAPAAGDRAAAKKPVGPV
                     AEALARGLALARRRAAAHALRETLRQQPADAPLLPLWNLLAEKNLP"
     gene            1108..>1916
                     /locus_tag="VGB85_24245"
     CDS             1108..>1916
                     /locus_tag="VGB85_24245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137225.1"
                     /translation="MLAPYAGTGHAAIGGEVDGLKARDQALERARKAALEAAIDQIGA
                     VAPAAKKQVLASHTVWTRSYRVLRQDDDGATATAVVSVEIDTAKLGKTLAGPASGTTT
                     ASPSFTVLPGLDLKSEGCPQGLEAEMKRALVGAGVVRDVAPGSAGPVLAASLQCTALG
                     VVSYPRQAVVRAELALRPTGTVGRPATTISATGFGEDVQAAASDALSGPGGLQAQVAK
                     TMSQRPDAGVQLKITAPWPAARVRRLERALRDSVVGVQAVTVAGVGSDGAVT"
BASE COUNT          238 a          634 c          802 g          242 t
ORIGIN      
        1 gggcaggttc ttctcggcga gcaggttcca cagcggcagc agcggcgcgt cggcgggctg
       61 ctggcgcagg gtctcgcgca gggcatgggc ggcggcgcgg cggcgggcga gcgcgaggcc
      121 gcgggcgagc gcctcggcga ccgggccgac cggcttcttg gcggcggcgc gatcacccgc
      181 ggccggcgcg atcaggcggt tgccaccgac gcgagatcgc aggcgggcgg ccacgccggc
      241 gccgagcggg gtcgtcggga cctcgtccgt cgcgggctcc tcggcaggcg tggaggcggg
      301 gccagcgacg ggacccgcgg cagggctcgg cgtgccgggg ctggtgaggg cgaattccgg
      361 gggacgggcg gggtcgtcga gatcgtcgcc acccgcggcg ccggggctgc gcacgcgctc
      421 gaccacgggg gtcgggaggt cgcggcgcca gcgacgacga ccgaggtccc acgcctcgtc
      481 gcgctcaccg gcgcggggtg ggggatcacc ggcggcgacc cgctgctcga gcgcctgacc
      541 gcgctgaagg atctcctcgc cgtggccgcg cggcaacacg gtgacgacgg tgtcctcctt
      601 gatgatcgcg tagagggtct cgccgaagct ctcgagcggg atcaggatct gcggctcggc
      661 gagcatgacg tcgagggtcc gcaccgcctt gccaccgtcc tctgcggtgg tgatcgcggc
      721 gtcgagacga tcgcgcaggg cctcgtcgtc ttcctcggcg accgtgggca cgagctcgcg
      781 caggcgctgc acggcgcggt gagtgatgct gtatcgccgg tgtccgggga aggggctccg
      841 aagcatgatc gcggccggtg tatcgcaggc ccgaggagaa tgctaccgcg cacgcgtgcg
      901 accggcgccg gggggcgcgg ggcctcgtgt ccatgggccc ccggatttac gtcgtatcct
      961 tgcgccacac gggcgatgcc tgccaagctt gccgcgatgc gacgcgtggt ccttgcagcg
     1021 gtgttcgggg cgactctggg gtccggggtg accttgggca cccctgcgct ggcagcgctg
     1081 ccgacgcaga caccggcgat gatcagcatg ttggccccgt acgcgggcac cgggcacgcg
     1141 gcgatcggcg gcgaggtcga cgggctcaag gcgagggacc aggcgctcga gcgggcgcgc
     1201 aaggcggcgc tcgaggcggc gatcgaccag atcggcgcgg tcgcaccggc ggccaagaag
     1261 caggtgctgg cgtcgcacac ggtgtggacg cggtcgtatc gggtgctgcg tcaggacgac
     1321 gacggcgcga ccgcgacggc ggtggtgtcg gtggagatcg acacggcgaa gctcggcaag
     1381 acgctcgccg ggcccgcgtc ggggacgacg acggcgagcc cgagcttcac ggtgctgccg
     1441 ggcctcgacc tcaagtccga gggctgtccg cagggcctcg aggccgagat gaagcgggcg
     1501 ctggtcggcg cgggggtcgt ccgcgacgtc gcgccggggt cggcggggcc ggtgctcgcg
     1561 gcgagcctcc agtgcacggc gctcggcgtg gtcagttatc cccggcaggc agtggtgcgc
     1621 gccgagctgg cgctgcggcc gaccggcacc gtcggccgtc cggcgacgac gatctcggcg
     1681 accggcttcg gcgaggacgt gcaggcggcc gcgagcgacg cgctcagcgg gcccggcggt
     1741 ctccaggcac aggtcgcaaa gaccatgtca caacggcccg atgcgggcgt gcagctcaag
     1801 atcacggcgc cgtggccggc cgcgcgggtg cgccggctcg agcgggcgct gcgagattcg
     1861 gtggtcggcg tgcaggcggt gacggtcgcc ggcgtcggca gcgacggggc ggtgac
//
LOCUS       DASWNJ010001458         2158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_338050, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001458 DASWNJ010000000
VERSION     DASWNJ010001458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338050"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VGB85_24250"
     CDS             complement(<1..186)
                     /locus_tag="VGB85_24250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0137226.1"
                     /translation="MSAEQPHVLIVEDEAAIRHGLVELLRAQDFALDLAADGHEALIK
                     LATGRYDVVLLDWMLPGV"
     gene            complement(339..1298)
                     /locus_tag="VGB85_24255"
     CDS             complement(339..1298)
                     /locus_tag="VGB85_24255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137227.1"
                     /translation="MAGHEEQRLRQFARLQKIARIYGGSLLSPVYFNNWTVLEFECGA
                     GHRFVSRPMRIFAGRWCSRCDGHTRRSLADCAEWARAKHGQCLALVYVHANDPMPWRC
                     AAGHEWMARPNRIRMGGWCPYCRGRGLRTHRELVAIARRFGGTCVTTHYDNYKEHLQW
                     RCEQGHEFLRTIADVRRGRWCQRCEGRTPWSLAELQRLAQERGGVCLSRRYENTESPM
                     RWRCAEGHVWTTAANHVTRGSWCAKCRLATRSYKPRLTLADMQATAASHGGVCLSKTY
                     RGSGVPLRWRCGAGHTWVARPNYVRLGWWCSRCKAEARAVKAT"
     gene            complement(1319..1645)
                     /locus_tag="VGB85_24260"
     CDS             complement(1319..1645)
                     /locus_tag="VGB85_24260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137228.1"
                     /translation="MLAALLAACGVTIPGAGTNDATGPPDITVSTSETDSSSQGTGAD
                     ATVSPDSTIDDSSKHDRTAAPVCGAGVIDPADFPSAGGSGPAPLAKRPSEDRPRRAPA
                     GARQRA"
     gene            complement(1668..>2158)
                     /locus_tag="VGB85_24265"
     CDS             complement(1668..>2158)
                     /locus_tag="VGB85_24265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137229.1"
                     /translation="DRIVFSSSVEYLGPDLEGLYGADQRCRMLAAVAMLPRFETYRAW
                     LSDSTTAAADRITHSKGRYILVNGLVVAADWDALISGTLENPINVTESSEVSVGSRVW
                     TGTLANGQPAYGSSFCADWTEEADFFDLAGSGIRNWTDASWSFFEQNACGSPSSIYCF
                     EN"
BASE COUNT          346 a          683 c          792 g          337 t
ORIGIN      
        1 gacgccgggc agcatccagt cgagcagcac gacgtcgtag cgtccggtcg ccagcttgat
       61 cagcgcctcg tggccgtcgg cggcgaggtc cagcgcgaag tcctgggcgc gcagcagctc
      121 gaccagaccg tgccggatcg cggcctcgtc ctcgacgatc aatacgtgcg gctgctcggc
      181 gctcaccctg acagcgtcgc agatccgggc gggggcggcg agcgtggtcg tgtaaggccg
      241 cggtaaaatc gcgcgtgcgc gtgcggatca cctgcaacga acgagaccac catgcaacgg
      301 agcagtggag cgacagagcc cctcgacccg cgtgacatct acgtggcctt gacggccctc
      361 gcttcggcct tgcagcgcga gcaccaccag cccaggcgca cgtagttcgg ccgggcgacc
      421 caggtatgac cggccccgca acgccagcgt agggggacgc cggagccgcg gtaggtcttc
      481 gacaggcaga caccgccgtg actggcggcg gtcgcttgca tgtcggcgag cgtcaatcgt
      541 ggtttatagc tgcgcgtggc cagccggcac tttgcgcacc agctgccgcg agtgacgtga
      601 ttcgcggctg tggtccagac gtggccctcg gcgcaccgcc agcgcatcgg ggactcggtg
      661 ttctcgtagc ggcgcgacag gcaaacgcca ccacgctcct gggcgaggcg ctggagctcg
      721 gcgagtgacc acggcgttcg gccctcgcaa cgctgacacc agcgcccccg acgaacatct
      781 gcgatcgtgc gcaggaattc gtggccctgt tcgcaacgcc actgcaaatg ctctttgtag
      841 ttgtcgtagt gcgtggtcac gcaggtgccg ccgaaccggc gggcgatggc gacgagctcg
      901 cggtgggttc gcaagccgcg gccgcggcag tacggacacc agccgcccat gcggatccga
      961 ttcggccgcg ccatccactc atggcccgcg gcacagcgcc agggcatcgg atcgttggcg
     1021 tgcacataga ccagcgccag gcactgccca tgcttcgcgc gggcccattc ggcgcagtcg
     1081 gcgaggctgc gtcgggtgtg gccgtcgcaa cgcgaacacc atcgaccggc gaagatccgc
     1141 atcggtcggg atacaaagcg gtggccggcg ccgcactcga actcgagcac tgtccagtta
     1201 ttgaagtaca cgggcgacag gaggctgccg ccgtagatcc gggcgatctt ctgcagtcgc
     1261 gcgaactgcc gcagtcgctg ctcctcgtgg ccggccatcg atcgaagttt cgcgggtttt
     1321 aggcgcgttg tcgagcgccg gccggcgccc gccgggggcg atcctcggat ggccgctttg
     1381 ccagaggggc tggtccagac ccacctgccg agggaaaatc tgcggggtcg atgacgccag
     1441 cgccgcaaac cggggcggcg gtccggtcgt gcttcgagct gtcatcgatc gtcgaatcgg
     1501 ggctgacggt ggcgtcggcg ccggtgccct ggctgctgga atccgtctcg ctggtggaga
     1561 cggtgatgtc gggaggtccg gtggcgtcgt tggtgccggc cccgggtatc gtcacgccac
     1621 aggcggcgag cagcgcggcg agcacaggga cgatacgggt catcgggtca attctcgaag
     1681 cagtagatcg aggatggtga tccgcaagcg ttctgctcga agaagctcca gcttgcgtcg
     1741 gtccagttgc ggatgcccga gccggcgaga tcaaaaaagt ccgcctcctc ggtccagtcg
     1801 gcgcagaacg aggagccgta ggcgggctgg ccgttggcga gggtgccggt ccatacgcgg
     1861 ctgccgacgg agacctcgga ggattcggtg acgttgatcg ggttctcgag ggtgccggag
     1921 atcagggcgt cccagtcggc ggcgacgacg aggccgttga cgaggatgta gcggcccttg
     1981 gagtgggtga tgcggtcggc ggcggcggtg gtggagtcgc tgagccaggc gcggtaggtc
     2041 tcgaacctgg gcaacatggc gacggcggcg agcatgcggc agcgctggtc ggcgccgtag
     2101 aggccctcga ggtccgggcc gaggtactcg acgctcgagg agaagacgat gcggtcct
//
LOCUS       DASWNJ010001459         6892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_26547, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001459 DASWNJ010000000
VERSION     DASWNJ010001459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26547"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VGB85_24270"
     CDS             complement(<1..446)
                     /locus_tag="VGB85_24270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137230.1"
                     /translation="MPRTVLVLALALLGCSPDKDASTGDVATTTGTTTGDGTSEAPTT
                     SGFDGVAAGCPGHPRTDACCCFEENPGPQGGKANTSSACGGQPLCPVMELACDSLDDS
                     CDNPITEDVTAIDCILQALANGASGQVAWAVVGGGGLWVNYVSLDLV"
     gene            complement(563..1693)
                     /locus_tag="VGB85_24275"
     CDS             complement(563..1693)
                     /locus_tag="VGB85_24275"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEY0137231.1"
                     /translation="MSQRRVLLAVLTLGCLGAACGETDPQGDTSGPGSSSGEASSGTA
                     TTGPTTTDTPTTDATTSAAETTSGASGDAGTTTDPTSTTSPTDATSTTTDATTGTGTG
                     AVDCGDGSLGAGEHTVEVEHDGVTRTALVHIPASVDLGAPAPLVLNFHGFTSNSAQQS
                     QFSNMNVTADAEGFIVAYPQGIDTSWNAGACCGNAMTQQVDDVGFVRKLVDALEQQLC
                     IDGKRVYATGMSNGGFMSHRLACEASDVFAAVAPVSAVNGMADCAPGRPVPVMMFNGT
                     ADILVAYNGGGNFDKSAPETFEEWADRDGCSDDAVPGKMAGLAACESHEACDAGVSVA
                     FCTFQGMGHCWPGQAICPYGASSTDLSANDEMWAFFSKYTLP"
     gene            complement(1733..2923)
                     /locus_tag="VGB85_24280"
     CDS             complement(1733..2923)
                     /locus_tag="VGB85_24280"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0137232.1"
                     /translation="MAEILVPFIVFFSVFGSIVAMRYLKYAHEARMKQLERGDGDGKQ
                     IKALEAQRNELDQRLRALETIICNVDFELNAKLNRLATQSLAMAGAGARPALAAADDA
                     GNLASGKIRAGTKVAGRFVIERPLGAGGMGAVYLARDEQLGEAVALKVIAGMALLDPG
                     SAERLRREASMARRISHPNVVRMHDLGEENGLLFVSMEYVAGESLAARIQRLGTLTPL
                     QLRPIVTQLCEGLTAAHGAGVIHRDLKPANVLIDANERVKLIDFGIARADLHAGMTAT
                     NMVIGTPEYMAPEQVKGGAVDARTDLYALGAVLYHALTGRPPFRGDTPIAVGLAHCTD
                     PVVPPRQLRPDLPAVWDAAIVRMLEKTPAARFASAQAVLAALSASDDSEAPTWQGPTP
                     TTRL"
     gene            complement(2985..3221)
                     /gene="rpmB"
                     /locus_tag="VGB85_24285"
     CDS             complement(2985..3221)
                     /gene="rpmB"
                     /locus_tag="VGB85_24285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018677334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEY0137233.1"
                     /translation="MSQVCQVTGKRPLAGMNVSHSHIRTHRRTMPNLHAKRYWVPSEN
                     RFISLRVSCHGMKIIDKRGIDVVLAEIRARGIKV"
     gene            3405..4025
                     /locus_tag="VGB85_24290"
     CDS             3405..4025
                     /locus_tag="VGB85_24290"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEY0137234.1"
                     /translation="MANTTYTPIEYKANDYSGLRGLHGITDEQIEVHLKLYNGYVTRT
                     NALLAKTAKMANEGQHADSSYQELKRRAGWEWNGMRLHEYYFDNLGASGALQAAQSPF
                     AARVEAQFGSVDAWKADLMGVAKMPGVGWAITYLDPKNGQIWNHWVNQHEVGHPSGAV
                     PLLVLDVWEHAFSVYRKPTDRPAYLEDFFANVSWDVVGKRLAIAGV"
     gene            complement(4097..4696)
                     /locus_tag="VGB85_24295"
     CDS             complement(4097..4696)
                     /locus_tag="VGB85_24295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137235.1"
                     /translation="MLLRSTLLLSSLITLAACSDDGGGSTSDASTGNTTPATSEPSTG
                     EAPTGTTSEPMTSPTSTTSTTEPATSTTDPATTTGDTDGTTGEDTTTGMAGPSWAVDV
                     YPVLNPPVMCDCHTPGSGNLKLGDPETAYMNLVGVPAAKGTLLRVEPGDASKSFLWHK
                     INGTQADVGGGSKMPLGAPDLPQETIDLIAAWIDAGAQP"
     gene            complement(4860..5426)
                     /locus_tag="VGB85_24300"
     CDS             complement(4860..5426)
                     /locus_tag="VGB85_24300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137236.1"
                     /translation="MTLDRNPWMLALALVFTAACGDDGDDTDGEDTDSAGMTSATATA
                     TATTPTGTTDPTSSTGPTTGDESSGTDPGTETGTVAVDYMADIQPIWDTKCVAGCHIE
                     SGIAQTNGPILTAEKSYGELIDVPSKTVNGIVQVKPGDPTTSYLWHKVNGTQANVGGL
                     GLQMPQGGMLSAEELATIEAWIVAGAKP"
     gene            complement(5508..5888)
                     /gene="apaG"
                     /locus_tag="VGB85_24305"
     CDS             complement(5508..5888)
                     /gene="apaG"
                     /locus_tag="VGB85_24305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEY0137237.1"
                     /translation="MSSFAETQGIRVVVRSRYVHEQSQPQHFVFSYTIKISNEGHDPA
                     QLRRRHWIITDATGGVREVEGEGVVGSQPLLHPGESFEYSGGCVLETPHGAMHGSYMM
                     LRPDGRCFEALIEPFVLAVPTAMN"
     gene            complement(5953..>6892)
                     /locus_tag="VGB85_24310"
     CDS             complement(5953..>6892)
                     /locus_tag="VGB85_24310"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0137238.1"
                     /translation="RRLAPEGLTAAAGHTRESCAGCTIGCDHRYGGQRLEYETQFALG
                     PLCGVEDPERLLAAARACDELGLDTISAGGTLAFAMECSERGLVDWPLRFGDDIVPWL
                     GRIARREGPGDLLAEGTRVAAGVIGRGSAAFACHVKGLELPGYEPRAMQTMALGLCVA
                     SRGADHNRSGAYQADLRPGVDRYHADPARAPALVVETEDQAALLDSLILCKFLRGAVP
                     EPWREGAELLAMVTGLAIDEPGLRRAVRRISELRHRFNLREGWTIAEDRLPDRFLDEA
                     LADGARLDRGEMAAQVAAYHVARGRDPDGAIPGGPL"
BASE COUNT          995 a         2323 c         2508 g         1066 t
ORIGIN      
        1 acgaggtcga gcgacacgta gttgacccac aggccaccgc cgccgacgac tgcccacgca
       61 acctgcccgc tcgcgccgtt ggcgagcgcc tgcaggatgc agtcgatcgc ggtcacgtcc
      121 tcggtgatcg ggttgtcgca ggaatcgtcc aggctgtcac aggcgagctc catcaccggg
      181 cacagcggct gcccgccgca ggcgctggac gtgttcgcct tgcccccctg cgggcccggg
      241 ttctcctcaa agcagcagca tgcgtcagtc cgcgggtgcc cgggacagcc ggcagcaacg
      301 ccatcgaagc ccgaggtcgt cggggcctcg gaggtgccat cccctgtcgt ggtcccggtc
      361 gtggtcgcaa catcgccggt cgatgcgtcc ttgtcggggc tgcagccgag cagcgcgagg
      421 gcgaggacga ggacggtgcg cggcatctcc ccagtaaaac acactcacgg cgctcgcgga
      481 cgtgcgcgac ggcgaggcac gcgagcgccg acgaacaacg ctacgcggcc cgacgacccc
      541 gtgcaccgga cacctggaat actcagggca gcgtgtactt gctgaagaag gcccacatct
      601 cgtcgttcgc cgagaggtcg gtgctcgagg cgccgtaggg gcagatcgcc tggccgggcc
      661 agcagtggcc catgccctga aaggtgcaga aggcgaccga gaccccggcg tcgcacgcct
      721 cgtggctctc gcacgcggcc aggccggcca tcttgccggg cacggcgtcg tcgctgcagc
      781 cgtcgcggtc ggcccactcc tcaaaggtct cgggcgcgct cttgtcgaag ttaccgccgc
      841 cgttgtacgc gaccaggatg tcggcggtgc cgttgaacat catcaccggc accgggcggc
      901 cgggcgcgca gtcggccatg ccgttgacgg cggacaccgg ggcgacggcg gcgaacacgt
      961 cggaggcctc gcaggcgagg cggtgcgaca tgaagccgcc gttcgacatg ccggtcgcgt
     1021 agacgcgctt gccgtcgatg cacagctgct gctcgagcgc atcgaccagc ttgcgcacga
     1081 agccgacgtc gtcgacctgc tgcgtcatcg cgttgccgca gcaggcgcca gcgttccacg
     1141 aggtgtcgat gccctgcgga taggcgacga tgaagccctc ggcgtcggcc gtcacgttca
     1201 tgttggagaa ctggctctgc tgcgccgagt tggaggtgaa gccgtggaag ttgagcacca
     1261 gcggcgccgg ggcgccgagg tcgacgctgg cagggatgtg tacgagggcc gtgcgtgtca
     1321 caccgtcgtg ctcgacctcg accgtgtgct cacccgcgcc gagcgagccg tcgccgcagt
     1381 cgaccgcgcc agtgccagtg ccggtggtcg cgtcggtcgt cgtgctcgtc gcgtccgtcg
     1441 gcgaggtcgt gctcgtcggg tcggtcgtcg tgcccgcgtc accggaggcg ccgctggtgg
     1501 tctcggccgc ggaggtggtc gcgtcggtgg tcggcgtgtc cgtggtcgtg ggtccggtcg
     1561 tcgcggtccc cgagctcgcc tcgccggacg agctgcccgg gcccgatgtg tcgccttgcg
     1621 ggtctgtttc gccgcacgcg gcgccgaggc aaccgagggt caagactgcg aggaggacgc
     1681 ggcgctgcga catgccggga gcttagccgg gtccggggcg cgtccgatag cctcacaggc
     1741 gggtcgtcgg cgtcgggcct tgccaggtcg gcgcctcgct gtcgtcgctg gcgctcagcg
     1801 ccgcgagcac cgcctgcgcg ctggcgaagc gggctgcggg ggtcttctcg agcatgcgca
     1861 cgatcgcggc gtcccacacg gccggaaggt cgggtcgcag ctgacgcggc ggcacgacgg
     1921 ggtcggtgca gtgcgcgagg ccgacggcga tcggtgtatc gccacgaaac ggcgggcggc
     1981 cggtcagcgc gtggtagagc acggcgccga gagcgtagag gtcggtgcgc gcgtcgacgg
     2041 ccccgccctt gacctgctcg ggcgccatgt actcgggggt gccgatgacc atgttggtcg
     2101 cggtcatgcc agcgtgcagg tcggcgcggg cgatgccgaa gtcgatcagc ttgacgcgct
     2161 cgttggcgtc gatgaggacg ttggcgggct tgaggtcgcg gtggatgacg ccggcgccgt
     2221 gcgcagcggt gagaccctcg cacagctggg tgacgatcgg ccgcagctgc aacggggtca
     2281 gggtcccgag ccgctggatg cgggccgcca ggctctcgcc ggcgacgtac tccatcgaca
     2341 cgaacagcag gccgttctcc tcgccgaggt catgcatgcg cacgacgttc gggtgggaga
     2401 tccgccgggc catgctggcc tcgcggcgca gtcgctcggc cgacccgggg tcgagcagcg
     2461 ccatgccggc gatcaccttg agcgcgacgg cctcgccgag ttgttcatcg cgcgcgaggt
     2521 agacggcgcc catgccaccg gcgccgagcg ggcgctcgat gacgaagcgc cccgcgacct
     2581 tggtgccggc gcggatcttc ccgctggcga ggttgccggc gtcatccgcc gcggcgagcg
     2641 ctggacgggc gccagcgccg gccatcgcca ggctctgcgt cgccagccgg ttgagcttgg
     2701 cgttgagctc gaagtcgacg ttgcagatga tcgtctccag cgcccgcaga cgctggtcga
     2761 gctcgttgcg ctgagcttcg agggccttga tctgcttgcc gtcgccgtcg ccccgctcga
     2821 gctgcttcat ccgggcctcg tgcgcgtact tgaggtagcg catcgccacg atcgatccga
     2881 agaccgagaa gaagacgatg aaggggacga ggatctcggc caaggggctc ctgaaggacc
     2941 cgctttggaa aacactcgag gcgggcgaga tctcgcccgc ggagctagac cttgatgccg
     3001 cgggcgcgga tctcggcgag gacgacgtcg atgccgcgct tgtcgatgat cttcatgccg
     3061 tggcagctga cgcgcagcga gatgaagcgg ttctcgctgg gcacccagta ccgcttggca
     3121 tgcagattcg gcatcgtgcg ccggtgcgta cggatgtgcg agtgagacac gttcatgccc
     3181 gcgagggggc gcttaccggt gacctggcag acttgcgaca tggggcgaag ggccttcgag
     3241 aagggagcgg gagataaaca ccggggggct cggagtgtca agtcgccggg gcatgatcga
     3301 tcgggtccaa tgcgggccgc ggatgagcgc atccgcacct gccggggggg cgcggccgtc
     3361 atccgtcggt cagccgacgc cggcccggtg atacgcttcg ctccatggcg aacacgacct
     3421 acaccccgat cgagtacaag gccaacgatt actccggcct gcgcggcctg cacggcatca
     3481 cggacgagca gatcgaggtc catctcaagc tctataacgg ctacgtcacc cgcacgaacg
     3541 cgctgctggc gaagacggcc aagatggcga acgagggcca gcacgccgat tccagctacc
     3601 aggagctcaa gcgccgggcc ggctgggagt ggaacggcat gcgcctccac gagtactact
     3661 tcgacaacct cggcgccagc ggcgctctcc aggcggcgca gagcccgttc gcggcgcgcg
     3721 tcgaggcgca gttcggcagc gtcgacgcat ggaaggccga cctgatgggc gtcgccaaga
     3781 tgccgggcgt cggctgggcg atcacctacc tcgacccgaa gaacgggcag atctggaacc
     3841 actgggtcaa tcagcacgag gtcggccacc cctcgggcgc cgtgccgctg ctcgtgctcg
     3901 acgtgtggga gcacgcgttc tcggtctatc gcaagccgac cgaccgcccc gcgtacctcg
     3961 aggacttctt cgccaacgtc tcgtgggacg tcgtcggcaa gcgcctcgcc atcgccgggg
     4021 tctgagctcg aggacacccg acgaggaggg ccgccctgcg tacgcgcggg cggcctttcg
     4081 ccgtccgggc gccgcgtcag ggctgcgcgc cggcgtcgat ccacgcggcg atcaggtcga
     4141 tcgtctcctg cggcaggtcg ggcgcgccga gcggcatctt gctgccgccg ccgacgtcgg
     4201 cctgcgtgcc gttgatcttg tgccacaaaa agctcttgct ggcgtcgccc ggctcgaccc
     4261 gcaggagcgt gcccttggcc gccggcacgc cgaccaggtt catgtacgcg gtctcagggt
     4321 cgccgagctt caggttgccg gagcctggcg tgtggcagtc gcacatgacc ggcggattga
     4381 gcaccggata gacgtcgacc gcccagctcg gacctgccat cccggtcgtc gtgtcctcgc
     4441 cggtcgtgcc gtccgtgtcg ccggtggtcg tcgcggggtc ggtggtgctc gtcgcgggct
     4501 ccgtcgtgct cgtcgtgctc gtcgggcttg tcatgggctc gctggtcgta ccggtcggcg
     4561 cttcgccggt gctcggctcg ctggtcgcag gggtcgtatt gccggtcgag gcgtcgctgg
     4621 tcgagccacc accatcgtcg ctgcaggcgg ccagggtgat cagcgacgag agcaggagag
     4681 tgctacgaag aagcatggcg taagtccctg tcgagaggtg cgcgaaggtg cacgccagcg
     4741 ggctgcggtg gccaggcatg gtacctgcga tccaatgaaa aacgccgccc tatcgggcgg
     4801 cgtcgttcat gatcgtggac ctgtgaagca gcgcgagtcg aacgatcgcg cggccggctt
     4861 cagggcttgg cgccagcgac gatccacgcc tcgatcgtgg cgagttcttc ggccgacagc
     4921 atgccgcctt gcggcatctg gaggccgagg cccccgacgt tcgcctgcgt gccgttgacc
     4981 ttgtgccaca ggtagctggt ggtggggtcg ccgggcttga cctggacgat gccgttcacg
     5041 gtcttggacg gcacgtcgat cagctcgccg taggacttct ccgcggtgag gatcgggccg
     5101 ttggtctgcg cgatgccgga ctcgatgtga cagccggcga cacacttcgt gtcccagatc
     5161 ggctggatgt ccgccatgta atcgaccgcg acggtgccgg tctcggtgcc ggggtcggtg
     5221 ccgctcgact cgtcgcccgt cgtcgggccg gtcgagctcg tcgggtcggt cgtgcccgtc
     5281 ggggtggtgg cagtggcagt ggcggtggcg ctggtcatgc cggcgctatc ggtgtcctcg
     5341 ccatcggtgt cgtcaccgtc gtcgccgcaa gcggccgtga atacgagcgc cagagcgagc
     5401 atccagggat tacgatcaag agtcatggtc ggggacggat accattcttg gcgtccaccg
     5461 atcaaggaag cgatcgcaga agcccgcggc gtgaatgagc gttcgcgtca gttcatcgcg
     5521 gtcggcaccg cgagcacgaa gggctcgatc agcgcctcga agcagcgacc gtcggggcgc
     5581 aacatcatgt agctgccatg catcgcgccg tgtggggtct ccagcacaca gccgccggag
     5641 tactcgaagg actcgcccgg gtgaagcagc ggttgcgagc cgacgacgcc ctcaccctcg
     5701 acctcgcgca cgcccccggt cgcgtcggtg atgatccagt ggcgacgccg cagctgcgcg
     5761 gggtcgtgcc cctcgttgga gatcttgatc gtgtagctga acacgaagtg ctgcggctgc
     5821 gactgctcgt gcacgtaacg cgaacgaacg acgacgcgga tcccctgagt ctcggcgaag
     5881 ctggacaccc ggtcgccata cccaaaacat tcgcgggtgc gcaagccccc gcgtacagct
     5941 gaccgccgtg cgtcagagcg gcccgcccgg aattgcgccg tcggggtccc ggccgcgtgc
     6001 gacgtggtat gcggccacct gggcggccat ctcgccgcgg tcgagccgcg cgccgtcggc
     6061 gagggcctcg tcgaggaagc ggtcgggcag gcggtcctcg gcgatcgtcc agccctcccg
     6121 caggttgaag cggtgacgca gctccgagat ccggcgcaca gcccgacgca gcccgggctc
     6181 gtcgatcgcc agcccggtca ccatcgccag cagctcggcg ccctcgcgcc acggctcggg
     6241 cacggctccg cgcaggaact tgcagaggat cagcgagtcg agcagcgccg cctgatcctc
     6301 ggtctcgacc acgagcgcgg gcgcgcgcgc cgggtcggcg tggtatcggt cgaccccggg
     6361 ccgcaggtcg gcctgatacg cgccgctgcg gttgtggtcg gcgccgcggc tggcgacgca
     6421 cagaccgagc gccatggtct gcatcgcccg cggctcgtaa cccgggagct cgaggccctt
     6481 cacgtgacag gcgaaggccg cgctgccgcg cccgatcacg ccggcggcca cacgtgtccc
     6541 ttcggccagg agatcgccgg gaccctcgcg ccgggcgatg cgcccgagcc agggcacgat
     6601 gtcgtcgccg aagcgcagcg gccagtcgac gaggccgcgc tcgctgcact ccatcgcgaa
     6661 cgccagggtg ccgccggcgg agatcgtgtc gaggccgagc tcgtcgcagg cccgcgccgc
     6721 cgcgagcagg cgctccgggt cctcgacgcc gcacaggggc ccgagcgcga attgggtctc
     6781 gtactcgagg cgctgcccgc catagcgatg gtcgcagccg atcgtgcagc cggcgcagct
     6841 ctcccgggtg tggccggccg cggcggtcag gccctcgggc gcgagccggc gc
//
LOCUS       DASWNJ010001460         2653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_485217, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001460 DASWNJ010000000
VERSION     DASWNJ010001460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485217"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="VGB85_24315"
     CDS             <1..776
                     /locus_tag="VGB85_24315"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEY0137239.1"
                     /translation="CAGPVASDHPAPGEWPLPAALRLARWSLGAAEVDRYRALGRDAA
                     TAMSEVLRAARPEWTGHQLAGASAEALWARGIHPALVLVGDARRLPLYRHPTPAADRI
                     GERVMLVFCARRHGLYANLTRSLYFRPPSAEERRAGEIVAEVEADALDASRPGASLGE
                     VFAAIVAGYARAGEGGAEAGHHQGGPCGYLSREAIARPDDPQTLVSDGAVAWNPSLPG
                     AKIEDTALVRADGLEILTTDPSWPTVQVRGRARPTVLER"
     gene            932..>2653
                     /locus_tag="VGB85_24320"
     CDS             932..>2653
                     /locus_tag="VGB85_24320"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY0137240.1"
                     /translation="MSLASCGDDMGTSSGGVTDSGTGLGSTGAQGSTSSPTGPTSGAT
                     DGSAGTATEGGATESGTGTTTGTTMTTASTTMGVTTGPTEGTATTGSSDPSNPSDPSD
                     PSDPSDPSNPSDPSAASDTDTGVSGTTGEGQTDTMMVSSGTSTGGTDTGGMVCAADKQ
                     CNDDCCADDQACVEAQCQKDCGGPPPCGPQQDCCAGGELCYLGECVTPGAVCDTQQCA
                     TKLQNDCGPGKVCDPELKLCLPSKADLNCTYKPPAQTFKPVPTFTWGARKVFACNVDA
                     DCQTAESCTKKICVPTWPHLKIAADDKPANNQSSSSAVVVDLDQDCIPEIVFNTYVAG
                     EANADGVLRAIRGDNGAKVWTVTDVNYQTNSTANPAVGDLDYDGKVEILVPGETKNIL
                     AFKWDGTPLWKSDLFTAANTSSAVAIANLDNDGDAEVIYADAVFDSKGKKLFEGTVGE
                     GLNGQGPISCVADLDGDGRQELIAGRTAYSFTGTVQGGNFTAKQVWNSAVGDGFCGIA
                     DFNGDKKPEVIVVSSGKIFALNGLTGVKLAEGLIPNGGKGGPPNIADFDGDGTPEVAA
                     AGSSQYIV"
BASE COUNT          437 a          932 c          971 g          313 t
ORIGIN      
        1 gctgcgccgg gccggtcgcc tccgaccacc ctgcccccgg cgagtggccg ttgccggccg
       61 cgctgcgcct ggcgaggtgg tcgctgggcg ccgcggaggt cgatcgctat cgggcgctcg
      121 gacgcgacgc ggcgacggcg atgagcgagg tgctgcgcgc ggcccgaccg gagtggacgg
      181 gccaccagct cgcgggcgcc agcgccgagg cgctgtgggc ccgcgggatc caccctgccc
      241 tcgtgctcgt cggcgacgcc cgccgcctgc cgctgtatcg ccacccgaca cccgcggccg
      301 atcggatcgg cgagcgcgtg atgctggtgt tctgcgcgcg ccgtcacggg ctctacgcca
      361 acctgacgcg ctcgctgtac tttcgcccgc cgagcgccga ggagcggcgc gccggcgaga
      421 tcgtcgccga ggtggaggcc gacgcgctcg acgccagtcg ccccggcgcg agcctcggcg
      481 aggtgttcgc ggcgatcgtc gccggctacg cgcgcgcggg tgaaggcggc gccgaggccg
      541 gacatcatca aggtgggccg tgcggctacc tctcgcgcga ggcgatcgcc cgcccggacg
      601 acccgcagac gctggtgagc gacggcgccg tcgcgtggaa cccctcgctg cccggcgcga
      661 agatcgagga cacggcgctg gtgcgtgccg acggcctcga gatcctgacg acggatccga
      721 gctggccgac ggtgcaggtc cgcggccgcg cgcgaccgac cgtgctcgag cgctagcgcg
      781 cctgcgatcc acctgtctct cgggtcaggt ggcaatgtgt tcgcacgcgc cgcgcttgtg
      841 cgaccgacgg cgctcatgcc accatcggcg aatggtaatg gcatggggtg gatggaacgg
      901 ggtggtcgtg cgcgtggcga tcggcgcggc tgtgtcgctg gcttcgtgcg gcgacgacat
      961 gggcacgagc agcgggggcg tcaccgatag cggcacgggg ctcggcagca ccggcgcgca
     1021 gggctcgacg agctcgccga ccgggccgac ctcgggcgcg accgacggca gcgccggcac
     1081 ggcgaccgag ggcggcgcga cggagagcgg caccggcacg accaccggca cgaccatgac
     1141 cactgcgtcg acgacgatgg gcgtgacgac cgggccgacc gaggggaccg cgacgaccgg
     1201 gtcgagcgac ccgagcaacc cgagcgaccc gagcgacccg agcgacccga gcgacccgag
     1261 caacccgagc gacccgagcg cggccagtga caccgacacg ggcgtcagcg ggacgacggg
     1321 cgagggccag acggacacga tgatggtctc gtccggcacg agcaccggcg ggacggatac
     1381 cggcggcatg gtgtgcgcgg ccgacaagca gtgcaacgac gactgctgcg ccgacgacca
     1441 ggcgtgcgtc gaggcccagt gccaaaagga ctgcggcggg ccgccgccct gcgggccgca
     1501 gcaggactgc tgcgccggcg gcgagctgtg ttacctcggc gagtgcgtga cgccgggcgc
     1561 ggtgtgtgac acgcagcagt gcgcgaccaa gctgcagaac gactgcgggc cgggcaaggt
     1621 gtgcgacccg gagctcaagc tgtgcctgcc gtcgaaggcc gacctcaact gcacgtacaa
     1681 gccgccggcc cagacgttca agccggtgcc gacgttcacg tggggcgcgc gcaaggtgtt
     1741 cgcgtgcaac gtcgacgccg actgccagac cgccgagagc tgcaccaaga agatctgcgt
     1801 gccgacctgg ccgcacctca agatcgcggc cgacgacaag ccagcgaata accagagctc
     1861 ctcgagcgcc gtcgtggtcg acctcgatca ggactgcatc ccggagatcg tcttcaacac
     1921 ctacgtcgcc ggcgaagcca acgcggatgg cgtcctgcgg gcgatccgcg gcgacaacgg
     1981 ggccaaggtc tggaccgtca ccgacgtcaa ctaccagacc aacagcaccg ccaacccggc
     2041 ggtcggtgac ctcgactacg acggcaaggt cgagatcctg gtccccggcg agaccaagaa
     2101 catcctcgcg ttcaagtggg acggcacccc gctgtggaag tcggacctgt ttacggcggc
     2161 caacacgagc agcgcggtcg ctatcgccaa cctcgacaac gacggcgacg ccgaggtgat
     2221 ctacgccgac gcggtgttcg actcgaaggg caagaagctg ttcgagggca cggtcggcga
     2281 gggcctcaac ggtcagggcc cgatctcgtg cgtcgccgac ctcgacggcg acggccgcca
     2341 ggagctgatc gccgggcgca ccgcgtacag cttcaccggc acggtccagg gcggcaactt
     2401 caccgccaag caggtatgga acagcgccgt cggcgacggc ttttgcggga tcgcggactt
     2461 caacggcgac aagaagcccg aggtgatcgt cgtctcgagc ggcaagatct tcgcgctcaa
     2521 cggcctgacc ggcgtcaagc tggccgaggg cctgatcccc aacggcggca agggcggacc
     2581 gcccaacatc gccgacttcg acggcgacgg cacccccgag gtcgccgcgg ccgggtcgag
     2641 ccagtacatc gtc
//
LOCUS       DASWNJ010001461         2799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_485257, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001461 DASWNJ010000000
VERSION     DASWNJ010001461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485257"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2799
                     /locus_tag="VGB85_24325"
     CDS             <1..>2799
                     /locus_tag="VGB85_24325"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0137241.1"
                     /translation="QSMWHLVYEAAQRHGDAEALVGKLERFLADPKHAGDAEGWRTLA
                     RAQTATGGDPVPAWRKALAADPNDPDARAALVGALESAGDAEAALAEFRRAGARGAER
                     VQLGLDLAGRLIASGKRAQGLEVAAEVEAAAGTAGNPLLRLLDFYNLNDEPARALAIA
                     QRLVKAHPRDPDARIALGEQLFQMGREADALKEWAVLPGLIRPAHAGWARHAEILSEH
                     RRPEAAISLEKALAAAPKDPKYLRLRALLEQDDRVPQRALATWQQVFELTRAADQRLL
                     HDEARTRMVDLLVGGTSFSQFSSRRVAIEKQALADLDGKDPNLALEAGLLLVELYTRE
                     EKFGRAVGVHEKLVKLRPQDGERLAELALALRRAGRGDAAMATLEQLMSLDPKRSADV
                     LAELAELAFDTGDIDRVLAAAARSEGAGPEHARVLVRLGELYERRGDLDEAAKTYAGI
                     LASDPKHPQARLKMAELTLSRGDVREAEAQLRAILGDGGTPEVLEEAGRRALDLAEAS
                     GTTLALLDLSLLRIRREPGDEAPRELMLDCLDRSDPRELHGWVRDDTAPARKQNLRRA
                     LVEALGRGPVGARLRAAEHLGRLELPQTAVPLARMGAQLTAPRDATRTVREAFEQARA
                     AAIRAAGELDDPEAVAVFGDLLASSDSSRESWYAAAWSLARSSAPAAAEHMRRFAVPE
                     QEGLIATLACAALARTGTPGSAGDLPRVSRMAAQTSHALQRRVCSFAEAALTPDDQVE
                     RMYDRLQAADPTLAAIAAWRIGQVDRERRPRAAVAALLRRYVGVPGLARDASAAALAR
                     QLGPAPAGTGATHLSSGQPRVAPLPPVRKSGWEITVERWLTGVIAPEYTPLAPDALTP
                     FRAELGQALAAAQAGPRAEAQSAKALQTAANCPLGPQAPGPGCLDLRPLVRPSPTDER
                     AGAPGPQGHSVAAKPRPA"
BASE COUNT          346 a         1128 c         1030 g          295 t
ORIGIN      
        1 gccagtcgat gtggcacctc gtctacgagg ccgcgcagcg ccatggcgac gccgaggcgc
       61 tcgtcggcaa gctcgagcgc ttcctcgccg accccaaaca cgccggcgac gccgagggct
      121 ggcggacgct cgcgcgggcc cagacggcga ccggcggcga ccccgtgccc gcgtggcgca
      181 aggcgctggc ggccgacccg aacgaccccg acgcccgcgc cgcgctggtc ggcgcgctcg
      241 agtcggccgg cgacgccgag gccgcgctgg ccgagtttcg ccgcgccggc gcccgcgggg
      301 ccgagcgcgt gcagctcggc ctcgatctcg ccggtcgcct gatcgcctcg ggcaagcgcg
      361 cgcagggcct cgaggtcgcg gccgaggtcg aggccgcggc cggcaccgcc ggcaacccgc
      421 tgctgcgcct gctcgacttc tacaacctca acgacgagcc ggcgcgcgcc ctggcgatcg
      481 cgcagcggct ggtgaaggcc cacccgcgcg accccgacgc gcggatcgcc ctcggcgagc
      541 agctgttcca gatgggccgc gaggccgacg cgctcaagga atgggcggtg ctgccgggcc
      601 tgatccgccc ggcgcacgcc ggctgggcgc ggcacgccga gatcctcagc gagcaccgcc
      661 ggcccgaggc ggcaatttcg ctcgagaagg ccctcgccgc cgccccgaag gacccgaagt
      721 acctgcgcct gcgcgccttg ttggagcagg acgatcgcgt gccgcagcgg gccctggcga
      781 cctggcagca ggtcttcgag ctgacccgcg ccgcggacca gcgcctgctg cacgacgagg
      841 cgcgcacgcg catggtcgac ctgctcgtcg gcggcaccag cttctcgcag ttctcgagcc
      901 ggcgcgtcgc tatcgagaag caggcgctcg ccgacctcga cggcaaggac cccaacctgg
      961 ccctcgaggc aggtctgttg ctcgtcgagc tctacacccg cgaggagaag ttcgggcgcg
     1021 ccgtcggggt ccacgagaag ctcgtcaagc tgcgcccgca ggacggcgag cggctcgccg
     1081 agctcgcgct cgcgctgcgc cgcgccggcc ggggtgacgc cgcgatggcg accctcgagc
     1141 agctgatgag cctcgacccc aagcgcagcg ccgacgtgct cgccgagctc gccgagctcg
     1201 cgttcgacac cggcgacatc gaccgcgtgc tcgcggccgc ggcccgcagc gagggcgccg
     1261 gcccggagca cgcgcgcgtg ctcgtccgcc tcggcgagct gtacgagcgc cgcggcgacc
     1321 tcgacgaggc cgccaagacc tacgccggaa ttctcgcgag cgaccccaag catccgcagg
     1381 cgcgtctgaa gatggccgag ctgacgctca gccgcggcga cgtgcgcgag gccgaggccc
     1441 agctgcgggc gatcctcggc gacggtggca ccccggaggt gctcgaggag gccggccgcc
     1501 gcgccctcga cctcgccgag gccagcggca ccacgctcgc gctgctcgac ctctcgctgc
     1561 tgcggatccg tcgcgagccg ggcgacgagg ccccgcgcga gctgatgctc gactgcctcg
     1621 accgcagcga cccccgtgag ctgcacggct gggtccgcga cgacaccgcc ccggcgcgca
     1681 agcagaacct gcgccgcgcg ctcgtcgagg cgctcgggcg cggcccggtc ggcgcgcgcc
     1741 tgcgggccgc cgagcacctc ggtcgtctcg agctgccgca gacggccgtg ccgctggcgc
     1801 gcatgggcgc gcagctgacc gcgccgcgcg acgcgacccg caccgtgcgc gaggccttcg
     1861 agcaggcgcg cgccgccgcg atccgggccg ccggcgagct cgacgacccc gaggccgtcg
     1921 ccgtgttcgg cgacctgctg gcgagcagtg attccagccg cgagtcctgg tacgccgccg
     1981 cgtggtcgct ggcccgcagc agcgcgcccg cggccgccga gcacatgcgt cgcttcgcgg
     2041 tgcccgagca ggagggcctg atcgcaaccc tcgcctgcgc cgcgctcgcg cgcaccggca
     2101 cgcccggcag cgccggcgac ctgccgcggg tgtctcgcat ggcggcgcag accagccacg
     2161 cgctgcagcg ccgggtgtgc agcttcgccg aggccgcgct gacgcccgac gatcaggtcg
     2221 aacgcatgta cgatcgcctg caggccgccg acccgacgct cgcggcgatc gccgcctggc
     2281 ggatcggaca ggtcgaccgc gagcgccgtc cgcgggccgc cgtcgccgcg ctgctgcgcc
     2341 gctacgtcgg cgtgccgggg ctcgcccgcg acgcctcggc ggccgcgctc gcccgccagc
     2401 tcggcccggc gcccgccggg acgggcgcga cccacctgtc ctccgggcaa ccccgggtcg
     2461 ccccgctgcc gccggtgcgc aagagcggct gggagatcac cgtggaacgc tggctcaccg
     2521 gcgtgatcgc gccggagtac acgccgctcg cccccgacgc gctgacgccg tttcgagccg
     2581 agctcgggca agccctcgcg gccgcgcagg ccggcccgcg cgcggaggcg cagtcggcaa
     2641 aggcgctcca gacggccgcg aactgccccc tgggcccgca ggcgcccgga ccgggctgcc
     2701 tcgacctgcg ccccctggtg cggccgtccc cgacggacga gcgggccggc gcccccgggc
     2761 cccaggggca ttccgtggcg gcgaaaccgc ggccggcgc
//
LOCUS       DASWNJ010001462         2294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_485283, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001462 DASWNJ010000000
VERSION     DASWNJ010001462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485283"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1452
                     /locus_tag="VGB85_24330"
     CDS             <1..1452
                     /locus_tag="VGB85_24330"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0137242.1"
                     /translation="VGAGDRVAYLTPNIPEALVAQFAVPLARAVLVALNTRLAAPEVG
                     YILDHSGATIVVVDAELLGVLQQSLSEAPGIRTVVVVGADPTDLPESSDALEFTTHGD
                     FLAGGDDDAVHWSVDDEYASIALNYTSGTTGRPKGVVYTHRGAYLNALGEIIHSGHSS
                     DSVYLWTLPMFHCNGWCTGWAVTAIGGTHVCLREVRAEVIWELIETEGISHLNGAPTV
                     LTTISSSPAARRLDRSITVTTAGAPPAPATIGQMEALGFSIVHVYGLTETYGPYSVCQ
                     PQDSWAQLDEDQRAARKARQGVGMIQAERLRVVDSDMQDVPADGETMGEVVMRGNNVM
                     AGYFDDPEGTAKAFTGGWFHSGDLGVMHPDGYVELRDRAKDVVISGGENISTIEVEQA
                     LMAHPQVSEAAVIGVPDERWGERPKAFVVLRPSAQVGEDELIEHVRSRIARYKAPRNV
                     DFVLELPKTSTGKIQKFALREAEWSGHASRIQG"
     gene            1457..>2294
                     /locus_tag="VGB85_24335"
     CDS             1457..>2294
                     /locus_tag="VGB85_24335"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0137243.1"
                     /translation="MTGIAPQGFVAPGYEGVREEFARNFTQRGDVGASCAVHDGTRFV
                     VDLWGGDATARRAWASDTVCLAYSVTKGLVTILVARLVDEGLLALDEPVTTYWPEFGA
                     EGKAATTVRMVLSHRAGLPVIEPGLDRAALLEPGVAAARLAAQRPAWEPGESFGYHAL
                     TWGWLVDELVLRVTGDRVKEAIQQKLAAPLAIDLHVGLPPSALPRVADLVVGSPGPTG
                     EGASADAVAPPADFDRALTAHGAFPLLDPVTWNDDAVRLASVPGAGVITNARAAARLY
                     AAV"
BASE COUNT          336 a          780 c          839 g          339 t
ORIGIN      
        1 gtcggggcgg gggaccgggt cgcctacctg acgcccaaca tcccggaagc actcgtggcc
       61 cagttcgccg tgccgctggc acgcgcagtg ctcgtcgcgc tcaacacgcg gctcgccgcg
      121 cccgaggtgg gctacatcct cgaccactcg ggggcgacga tcgtcgtggt cgacgccgag
      181 ctcctcgggg ttctgcagca gtcgctgtcg gaggctccgg gcatccgcac ggtcgtggtg
      241 gtgggggcgg atccgaccga tctgcccgag tcgtcggacg ctctcgagtt cacgacccac
      301 ggcgacttcc tcgcgggtgg ggacgacgac gccgtgcact ggtcggtcga cgacgagtac
      361 gcatcgatcg cgctcaacta cacgtccggc accacgggcc gcccgaaggg cgtggtctac
      421 acgcaccggg gcgcatacct caacgcgctg ggcgagatca tccactcggg ccactcgtcc
      481 gacagcgtct acctgtggac gctgccgatg ttccactgca acggctggtg cacgggctgg
      541 gccgtcacgg cgatcggcgg gacccacgtg tgcctgcgcg aggtccgggc cgaggtcatc
      601 tgggagctga tcgagaccga gggcatcagc cacctcaacg gtgcgccgac ggtcctcacg
      661 accatcagca gctcacctgc tgccaggcgg ctcgaccggt cgatcacggt gacgaccgcc
      721 ggagcgccgc cggctccggc gaccatcggg cagatggagg ctctcggctt ctccatcgtg
      781 cacgtctacg gactgaccga gacctacggt ccctactccg tgtgccagcc acaggactcc
      841 tgggcgcagc tggacgagga ccagcgcgcg gcccgcaagg cccggcaggg cgtcgggatg
      901 atccaggccg agcggctgcg cgtcgtcgac agcgacatgc aggacgttcc tgcggacggc
      961 gagacgatgg gcgaggtcgt gatgcgcggc aacaacgtca tggccggcta cttcgacgac
     1021 cccgagggca cggccaaggc attcacgggt gggtggttcc acagcggtga cctcggcgtc
     1081 atgcaccccg atggttacgt cgagctgcgt gatcgcgcca aggacgtcgt catctcgggc
     1141 ggcgagaaca tctcgacgat cgaggtcgag caggccctga tggcgcaccc ccaggtgagc
     1201 gaggccgccg tgatcggcgt gcccgacgag cgatgggggg agcggcccaa ggcattcgtg
     1261 gtcctgcgcc ccagcgcgca ggtcggtgag gacgagctga tcgagcacgt ccgatcccgg
     1321 atcgcgcgct acaaggcgcc gcgcaacgtc gacttcgtcc tcgagctgcc gaagacgtcc
     1381 accggcaaga tccagaagtt cgcgctgcgc gaggccgagt ggtctggcca cgcgagccgg
     1441 atccagggct gacacgatga ccgggatcgc gcctcagggg ttcgtcgccc ctggctacga
     1501 aggggtccgc gaggagttcg cgaggaactt cacgcagcgc ggcgatgtcg gtgcctcgtg
     1561 tgcggtgcac gacggcaccc gcttcgtggt ggacctgtgg ggcggcgacg cgaccgcgcg
     1621 gcgagcctgg gcgtccgaca cggtgtgcct ggcctactcg gtgacgaagg gtctggtcac
     1681 gatcctggtg gcccgtctgg tcgacgaggg cctgctggcc ctcgacgaac cggtgacgac
     1741 ctactggccg gagttcggtg cggagggcaa ggcggcgacc accgtccgga tggtgctgtc
     1801 gcatcgagcc ggtctgccgg tgatcgagcc cgggctcgac cgagccgccc tgttggagcc
     1861 tggcgtggcg gccgctcgcc tcgctgccca acgccccgca tgggagccgg gcgagtcgtt
     1921 cggttaccac gccctcacgt gggggtggct cgtcgacgag ctggtgctcc gcgtgaccgg
     1981 cgaccgggtg aaggaggcga tccagcagaa gctggcggct cccctcgcga tcgacctgca
     2041 cgtcgggttg ccgccttcgg cgctgcctcg cgtggccgat ctcgtggtcg gcagcccggg
     2101 cccgaccgga gagggcgcct cggccgacgc ggtggcgccg ccggcggact tcgacagggc
     2161 gctgacggcg cacggcgcct tcccgctgct cgatccggtg acctggaacg acgacgcggt
     2221 gcggctcgcg agcgtgccag gggccggcgt gatcacgaat gctcgagcag ccgcgcggct
     2281 ctacgctgcg gtcg
//
LOCUS       DASWNJ010001463         2115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_557617, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001463 DASWNJ010000000
VERSION     DASWNJ010001463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557617"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1782
                     /locus_tag="VGB85_24340"
     CDS             <1..1782
                     /locus_tag="VGB85_24340"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0137244.1"
                     /translation="KARDGRIDILVGRELEIERTIQILCRRSKNNPLFVGDPGVGKTA
                     IAEGLALRIVNGEVPEVLKPAVIFALDMGALLAGTRYRGDFEERLKAVLTELEQNKNA
                     VLFIDEIHTVIGAGATSGGSLDASNILKPALASGDLRCIGSTTYKEFRNYFEKDRALV
                     RRFQKIDIPEPSEDEAVKILEGLKPSYEQHHQVKYSQGALESAVKLSTRYIHDRKLPD
                     KAIDIIDEVGAAQMLKPANLRKKTIRAKDIEEIVSKMTRIPVKTVNARDKEALKSLDQ
                     DLKGVVFGQDDAIEALAAAIKLSRAGLRDPVKPIGSYLFSGPTGVGKTEVAKQLAKVM
                     ACELVRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLLTDAVDQHRHCVLLLDEIEKAH
                     PDVFNILLQVMDYGKLTDNNGKQVDFRNVILIMTTNAGAADMAKAVFGFGKAQQREGE
                     DTDAIKRLFTPEFRNRLDAVIPFKGLSPVIIASVVDKFVKELGAQLADKRVSIEIDDE
                     AKAWLAQKGFDPLNGARPLARVIQDHIKRPLADELLFGKLAKGGRVLITVADAKLAFH
                     ITPAGGDTALLAPPDDEGAEERELEPA"
BASE COUNT          392 a          737 c          678 g          308 t
ORIGIN      
        1 aaggcccgcg acggccgcat cgacatcctg gtcggccgcg agctcgagat cgagcgcacc
       61 atccagatcc tctgccgccg ctcgaagaac aacccgctgt tcgtgggcga ccccggcgtc
      121 ggcaagaccg cgatcgcgga agggctggcg ctccggatcg tcaacggcga ggtgccggag
      181 gtcctcaagc ccgccgtgat cttcgccctc gacatgggcg cgctgctcgc cggcacgcgc
      241 taccgcggcg acttcgagga gcggctcaag gccgtgctca cggagctcga gcagaacaag
      301 aacgcggtcc tgttcatcga cgagatccac accgtcatcg gcgccggcgc cacctcgggc
      361 ggctcgctcg acgcgtccaa catcctcaag ccggcgctgg cctccggcga cctgcgctgc
      421 atcggctcga cgacctacaa ggagttccgc aactacttcg agaaggaccg ggcgctggtg
      481 cgccgcttcc agaagatcga catccccgag ccgtccgagg acgaggcggt caagatcctg
      541 gagggcctca agcccagcta cgagcagcac caccaggtga agtactcgca gggtgcgctg
      601 gagtccgccg tcaagctctc gacccgctac atccacgacc gcaagctgcc cgacaaggcc
      661 atcgacatca tcgacgaggt cggggcggcg cagatgctca agcccgcgaa cctgcgcaag
      721 aagaccatcc gcgccaagga catcgaggag atcgtctcca agatgacgcg catcccggtc
      781 aagaccgtga acgcgcgcga caaggaggcg ctcaagtctc tcgaccagga cctcaagggc
      841 gtggtcttcg gccaggacga cgcgatcgag gcgctggccg ccgccattaa gctcagccgc
      901 gccggcctgc gcgacccggt caagccgatc ggcagctacc tgttctccgg cccgaccggc
      961 gtcggcaaga ccgaggtggc caagcagctg gccaaggtca tggcctgcga gctggtccgc
     1021 ttcgacatgt ccgagtacat ggagcgccac gcggtcagcc gcctgatcgg ggcacccccg
     1081 ggctacgtcg gcttcgacca gggcgggctg ctcaccgacg cggtcgacca gcaccgccac
     1141 tgcgtcctgc tgctggacga gatcgagaag gcgcaccccg acgtcttcaa catcctcctg
     1201 caggtgatgg actacggcaa gctcaccgac aacaacggca agcaggtcga ctttcgcaac
     1261 gtcatcctga tcatgacgac gaacgccggt gccgccgaca tggccaaggc cgtgttcggg
     1321 ttcggcaagg cgcagcagcg cgagggcgag gacaccgacg ccatcaagcg cctgttcacc
     1381 cctgagttcc gcaaccggct cgacgcggtg atccccttca agggcctctc gcccgtcatc
     1441 atcgccagcg tcgtcgacaa gttcgtcaag gagctgggcg cccagctcgc cgacaagcgc
     1501 gtctcgatcg agatcgacga cgaggccaag gcttggctag cccagaaggg cttcgacccg
     1561 ctcaacggcg cccgcccgct ggcccgcgtg atccaggacc acatcaagcg cccgctcgcc
     1621 gacgagctcc tgttcggcaa gctcgccaag ggcggccgcg tcctgatcac cgtcgccgac
     1681 gctaagctcg ccttccacat cacccccgcc ggcggcgaca cggccctgct tgcgcccccg
     1741 gacgacgagg gggcggagga gcgggaactc gagccggcgt aggtcggtgg cgaatcgctt
     1801 ggcatagcga tcgcgtcggg aaaggctcac ccggtgcccg agagttcctc acggggcgct
     1861 tggattccgg gtgggccacc tctcctccct ggcaggctta tcacacgccc ggattcaagg
     1921 cgtgggaact gacatgcagc ccgtcaggat ggttcgacag actcgtcggg taccgcaggt
     1981 gacggtagat gtcgggaacg cgttcgcgcg ggttcgacta ccgtgaagcg gcgttggagt
     2041 ttggcgcctt gcgtgctgat gacattgatc acgcgtccga cgacatcgac gatggccatt
     2101 tgaggtagcc gcagg
//
LOCUS       DASWNJ010001464         2789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_485307, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001464 DASWNJ010000000
VERSION     DASWNJ010001464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485307"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..519)
                     /locus_tag="VGB85_24345"
     CDS             complement(217..519)
                     /locus_tag="VGB85_24345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEY0137245.1"
                     /translation="MATHITEECINCGACEPECPNEAISEGEDTYVIDPALCTECVGF
                     HDYEACQAVCPVECCLPDPQRRESEAELLERAAKLHPETNFPAVENLPANLSRFRK"
     gene            complement(695..1213)
                     /locus_tag="VGB85_24350"
     CDS             complement(695..1213)
                     /locus_tag="VGB85_24350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137246.1"
                     /translation="MSREPDEEPIFSGYSEFGAALAEFVGAELSAHGAILSDAEGDPI
                     DFAHRHGAVTPLDLQIAGAQVEQAATRMAAWCVRQGLGRCEILIEASHGLLLSAAPGD
                     GCVLTSLHAAPDRGGSRDDPTGPGAASPSRETGPAGEAAASGGAEGREDGLDGLLARF
                     SDLRHRIGGLIQ"
     gene            complement(1250..2368)
                     /locus_tag="VGB85_24355"
     CDS             complement(1250..2368)
                     /locus_tag="VGB85_24355"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HEY0137247.1"
                     /translation="MFRRRLGPAVGLAVGLGAVLLARPLVAEDRIVLRGNYYREQSTR
                     VLAPVVYVEKDVPDERFTVGAEYLLDAVTSASIGAGAAAVTGGDYLFTEFRHETTLRL
                     ASRLRNWQLGGFFRYSTESDWISRSFGASVARDVFGRAGNVSISYTGNFDSVNKIFAG
                     RVLPHKSTGDTNLQQVNFLGLGYTHALTPRLLGGVLAEGIIATGPQDNPYRQVRDGLP
                     ETHPLLRRRGAISGFLRYAVPRTPLVLEPRYRLYGDDWGIVAHGIDMRAHVRFLKRVI
                     FRARYRYYTQTGATFWAADGYYPATAEFRTADPKMSKFHSHTPGGELTIELDGLARRV
                     RGLHWLRGAWVQATYNHVFQTNRFGNARLGSLAFSVPF"
     gene            complement(2368..2571)
                     /locus_tag="VGB85_24360"
     CDS             complement(2368..2571)
                     /locus_tag="VGB85_24360"
                     /inference="COORDINATES: protein motif:HMM:NF025452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HEY0137248.1"
                     /translation="MPVVGVALALVLGGCATVKPSEREVLSRPEMNPAGEGLEEQFQS
                     HVESAREGGFGGHGAAGGGCGCG"
BASE COUNT          421 a          951 c         1029 g          388 t
ORIGIN      
        1 cgcgcgacga gcacgacgat caccgcggtg atcaccaccg atagcgagag gcgaagccac
       61 caccacggtg cggtgggact tcgcctcggc gcgggcctcg tgggctcgca agggatggct
      121 agaggtggtg agacagcgga gagggcgggg gcaacgctcc cgacgcgggc gtcgtaggcg
      181 tcgggggctt tcgggtcctc cgccgccgcc acggtctcac ttgcggaagc ggctgaggtt
      241 ggccgggagg ttctcgacgg ccgggaagtt ggtctccggg tggagcttcg cggcgcgctc
      301 gagcagctcg gcctcgctct cgcggcgctg cgggtcgggc aggcagcact cgaccgggca
      361 cacggcctgg caggcctcgt agtcgtggaa gccgacgcac tcggtgcaca gggccgggtc
      421 gatcacgtag gtatcttcgc cctccgagat ggcctcgttg gggcactccg gctcgcacgc
      481 gccgcagttg atgcactctt cagtgatgtg ggttgccatg ggaacgaact gactccttgc
      541 cgtcaggggc agagggtggt gccggcgcag gccgcgggac agtcttcgcg taaagccgac
      601 cccgggtcaa gctggccggg ccgcgcaggt cggctctcat aaactaccgg cgccgagaag
      661 tccatgcggg ttcgttctcg tcgtcgccgg accactactg gatcaagccg ccgatgcggt
      721 gacgcaggtc cgaaaagcgg gccagcaggc catcgaggcc gtcctcgcgc ccctcggcgc
      781 ccccgctcgc cgccgcctcg ccggccgggc cggtctcccg cgacggggac gcggcaccag
      841 ggccggtcgg atcatcgcgg ctgccgccgc ggtccggcgc ggcgtgcagg gaggtcagca
      901 cacagccgtc acccggcgca gcgctgagca gcaggccgtg acttgcctca atcaggatct
      961 cacagcgacc gaggccctga cggacgcacc aggccgccat gcgggtcgcc gcctgctcga
     1021 cctgcgcgcc ggcgatctgt agatccagcg gtgtcaccgc gccgtggcgg tgcgcgaagt
     1081 cgatggggtc cccctcggcg tcgctgagga tcgcgccgtg ggcgctgagc tccgcgccga
     1141 cgaactccgc cagcgccgcg ccgaactcgc tgtagccgct gaagatcggc tcctcatctg
     1201 gctctcggga catgttcgcc atcatcgccc gcccgatcag aaaggagcct cagaagggca
     1261 ccgagaaggc gaggctaccg aggcgcgcgt tgccgaagcg gttggtctgg aacacatggt
     1321 tgtaggtcgc ctggacccac gcgccgcgca gccagtgcag gcctcgcacg cgccgcgcga
     1381 ggccgtcgag ctcgatcgtc agctcgccgc ccggcgtgtg cgagtggaac ttgctcatct
     1441 tcgggtcggc ggtgcgaaac tcggccgtgg ccgggtagta gccgtcggcg gcccagaagg
     1501 tcgcgccggt ctgcgtgtag tagcgatacc tggcgcggaa gatgacgcgc ttgaggaagc
     1561 ggacgtgggc ccgcatgtcg atgccgtggg cgacgatgcc ccagtcgtcg ccatacaggc
     1621 gatagcgcgg ctcgagcacc agcggggtcc tcggcacggc gtagcgtaaa aagccactga
     1681 tcgccccgcg gcggcgcagc agcgggtggg tctcgggcag gccgtcgcgg acctggcggt
     1741 aggggttgtc ctgcggcccg gtggcgatga tgccctcggc cagcacgccg ccgagcaggc
     1801 gaggcgtcag cgcgtgggtg taaccgaggc cgaggaagtt gacctgctgg aggttcgtgt
     1861 cgccggtcga cttgtgcggc aggacccggc cggcgaagat cttgttcacg gagtcgaagt
     1921 tgccggtgta actgatgctg acgttgccgg cgcggccgaa cacgtcgcgc gcgaccgagg
     1981 ccccgaacga gcgcgagatc cagtcgctct cggtcgagta gcggaagaac ccgccgagct
     2041 gccaattgcg caggcgcgag gccaggcgca gcgtggtctc gtggcggaac tcggtgaaca
     2101 ggtagtcgcc gccggtcacc gccgccgcgc ccgcgccgat cgaggccgag gtcacggcgt
     2161 cgagcaggta ctcggcgccg accgtgaagc gctcgtcggg cacgtccttc tcgacgtaga
     2221 ccaccggcgc gagcacgcgg gtcgactgct cgcggtagta attgccgcgc agcacgatgc
     2281 ggtcctcggc gaccagcggc ctggcgagca gcaccgcacc gagcccgacc gcgagcccga
     2341 ccgccggacc gagccgtcgt ctgaacatca gccgcaaccg catccgccac ccgcggcgcc
     2401 gtgcccgcca aagccacctt cgcgggccga ctccacgtgc gactggaact gctcctcgag
     2461 gccctcgccc gcggggttca tctccggccg gctgagcacc tcgcgctcgg acggcttgac
     2521 cgtggcgcag ccgccgagga caagcgcgag cgcgacgccc acgactggca cgacaggaga
     2581 ggaccggagc gtggacatgg gccagcaagc tgccgcagcc ggacctttcc ggcaagaggg
     2641 aagcagggcc ccgcgcaggg cgcccgcgca ggctgcccgc gggtgggtgc acccgggttc
     2701 gtgtgtcggc ggtccggagc cgggcggcgc agacctgcct gtcgacttgt cagcgccccg
     2761 gcggttgtgg tctaaggcag gccgcgaat
//
LOCUS       DASWNJ010001465         1688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_408369, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001465 DASWNJ010000000
VERSION     DASWNJ010001465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408369"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /gene="glmM"
                     /locus_tag="VGB85_24365"
     CDS             <1..1321
                     /gene="glmM"
                     /locus_tag="VGB85_24365"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY0137249.1"
                     /translation="TDGLRGVAGTELTAELALALGRAAARVLGGPRFLIARDTRLSSS
                     WLEAALAAGIAAEGVDVELLGVLPTPGLALHSADEGVPAAMISASHNPFQDNGIKLFA
                     ADGFKLPDDVEAHLEALMAPGVLDEQWARGADIGRAVRIDDASGRYLTHVKQAFPTEL
                     ALDGLKVVVDCAHGAAYKVAPMVLRELGADVIAIGAAPDGLNINDNCGAVHPARMCAA
                     VREHGADLGIALDGDADRAVFGDRHGNLVDGDAILAICARELHARGALRGGAVVATIM
                     SNLGLGRCLREVGLDLVRTPVGDRYVVEQMISGGYNLGGEQSGHLVFLDHGTTGDGVV
                     AALQVLAIMQRQGKPLDDLTRVMTRYPQLLQGITVAEKIPLDRLPGLSAVIRGVEAEL
                     GSDGRVVVRYSGTEAKLRVMLEGPDQDRLEDYAGRIAEAARSELGAV"
     gene            complement(1322..>1688)
                     /locus_tag="VGB85_24370"
     CDS             complement(1322..>1688)
                     /locus_tag="VGB85_24370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0137250.1"
                     /translation="LVVEDEAGVRNLVKRVLDGLGYHVLTAREAGEALRICAEVVVDL
                     VLTDVVMPGLGGHALAERLQARRPTRVLFMSGYDPESPPMAAGGLLRKPFGPQELSAR
                     VREALDQPVPKVMSEKPAE"
BASE COUNT          224 a          666 c          573 g          225 t
ORIGIN      
        1 cacggacggc cttcgcggcg tcgctgggac ggagctcacc gccgagctcg cgctcgccct
       61 cgggcgcgcc gccgcccggg tgctcggcgg cccacgcttc ctgatcgccc gcgacacccg
      121 cctctcgagc tcctggctcg aggcggcgct ggccgccggc atcgccgccg agggcgtcga
      181 cgtcgagctg ctcggggtgc tcccgacgcc cggcctcgct ctgcactccg ccgacgaggg
      241 cgtgccggcg gcgatgatct cggcgtcgca caaccccttc caggacaacg gcatcaagct
      301 gttcgccgcc gatggcttca aacttccgga cgacgtcgag gcgcacctcg aggccctgat
      361 ggcgccgggg gtgctcgacg agcaatgggc gcgcggcgcc gacatcggcc gcgccgtccg
      421 catcgacgac gccagcggtc gctacctgac gcacgtcaag caggcgttcc cgaccgagct
      481 cgcgctcgac ggcctcaagg tcgtcgtcga ctgcgcgcac ggggccgcct acaaggtcgc
      541 gccgatggtg ctacgcgagc tcggggccga cgtgatcgcc atcggcgccg cacccgacgg
      601 cctcaacatc aacgacaact gcggcgccgt gcaccccgcg cgcatgtgcg ccgccgtgcg
      661 cgagcacggg gccgacctcg gcatcgcgct cgacggtgac gccgaccggg ccgtgttcgg
      721 cgaccggcac ggcaacctcg tcgacggcga cgccatcctc gcgatctgcg cgcgggagct
      781 gcacgcccgc ggggccctgc gcggcggggc cgtcgtcgcc acgatcatgt ccaacctcgg
      841 cctcggccgg tgcttgcgcg aggtcggcct cgacctcgtg cgcacccctg tcggcgaccg
      901 ttacgtcgtc gagcagatga tctccggcgg ctacaacctc ggcggtgagc agtccggcca
      961 cctcgtcttc ctcgatcacg gcaccaccgg cgacggcgtc gtcgcggccc tgcaagtgct
     1021 ggcgatcatg cagcgccagg gcaagccgct cgacgacctc acccgcgtga tgacccggta
     1081 cccgcagctg ctgcagggca tcaccgtcgc cgagaagatc ccgctcgacc gcctccccgg
     1141 gctctccgcc gtgatccgcg gcgtcgaggc cgagctcggc agcgacggtc gtgtcgtcgt
     1201 gcgctactcc ggcaccgagg ccaagctgcg cgtcatgctc gagggccccg atcaggaccg
     1261 cctcgaggac tacgccggtc ggatcgccga ggccgcgcgc agcgagctcg gcgcggtgta
     1321 gtcactcggc tggcttctcg gacatgacct tcgggacagg ctggtcgagc gcctcgcgca
     1381 cgcgagcgct cagctcctgc gggccgaagg gcttgcgcag cagcccgccg gcggccatcg
     1441 gcgggctctc cgggtcgtag ccggacatga acagcacccg cgtcggccgg cgcgcttgca
     1501 ggcgctccgc gagcgcgtgc ccgccgaggc ccggcatcac cacgtccgtc agcaccaggt
     1561 cgaccaccac ctccgcgcag atccgcagcg cctcgccggc ctcgcgcgcc gtcaacacgt
     1621 ggtagccgag gccgtcgagc acccgcttga ccaggttgcg cacccccgcc tcgtcctcga
     1681 cgaccagg
//
LOCUS       DASWNJ010001466         1710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_485413, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001466 DASWNJ010000000
VERSION     DASWNJ010001466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485413"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /gene="hisC"
                     /locus_tag="VGB85_24375"
     CDS             <1..1097
                     /gene="hisC"
                     /locus_tag="VGB85_24375"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY0137251.1"
                     /translation="MALPRPRPGIMEISPYVGGKATAATDRPIAKLSSNESPLGPSPK
                     AVAAYRELAGELHRYPDGNCAGLRHAIARQHNLDPDLVVCGAGSDEIIALLVKAYAGS
                     GDEVLHSAHGFLMYRLAALGEGAHPVAAPERGLKADVDALLASVTSRTRLVFLANPNN
                     PTGSYLNTDELRRLHAGLPGDVVLVVDAAYAEYVGAPDYASGLELAREAPNVVTTRTF
                     SKLHGLASLRVGWMTASPAIVDAVNRVRGPFNVSQAGQAAAVAALEDLEHQERARRHN
                     DVWLAWLAREITNLGLTVYPSVGNFLLIGFPTEPGRDATAANAYLEREGVIPRMMGSY
                     GLPHALRISVGLEDENRRVVEALGFFVGNG"
     gene            1114..>1710
                     /locus_tag="VGB85_24380"
     CDS             1114..>1710
                     /locus_tag="VGB85_24380"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEY0137252.1"
                     /translation="MEPFRRVAMLGIGLINGSLAKVLKREGAAGEIVACSRRAETRDR
                     ALELGLCDRAVADPADAVRGADLIVLGTPPGALSALAAAMSPGLDRDAIVTDVSSIKA
                     QVVRDVAPHLPDPSRFVPGHPVAGTEHSGPDAAFDTLFVRRRCILTPTEETDPQAVDR
                     VRAMWELAGSAVDVMTPEHHDRVLAITSHLPHLIAYTIV"
BASE COUNT          239 a          644 c          602 g          225 t
ORIGIN      
        1 cgatggccct cccccggccg cgtcccggca tcatggagat cagcccctac gtcggcggca
       61 aggccacggc cgcgaccgac cggccgatcg cgaagctgtc gagcaacgag agcccgctgg
      121 ggccgagccc gaaggcggtg gcggcctacc gcgaactcgc cggcgagctc caccgctatc
      181 ccgacggcaa ctgcgcgggc ctgcgccacg cgatcgcccg gcagcacaac ctcgaccccg
      241 acctagtggt gtgcggcgcc ggctcggacg agatcatcgc gctcctcgtc aaggcctacg
      301 ccgggtccgg tgacgaggtg ctccacagcg cgcacggctt cctgatgtac cggctcgcgg
      361 ccttgggcga gggcgcccac ccggtggcgg cgccggagcg ggggctgaag gccgacgtcg
      421 acgcgctgct ggcgagcgtc accagccgca cccggctcgt cttcctggcg aacccgaaca
      481 acccgaccgg cagctacctg aacaccgacg agttgcgccg cctccacgcc gggctgcccg
      541 gcgacgtggt cctggtcgtg gacgccgcct acgcggagta tgtcggggcg ccggactacg
      601 cgagcggcct ggaactcgcg cgcgaggcgc cgaacgtggt caccacgcgg accttctcga
      661 agctccacgg cttggcctcg ctcagggtcg gctggatgac agcctcgcct gccatcgtcg
      721 acgcggtcaa ccgcgtgcgc gggccgttca acgtgtcgca ggcgggacag gcggcggcgg
      781 tcgcggcgct ggaggacctg gagcaccagg agcgcgcccg ccgccacaac gacgtctggc
      841 tggcgtggct cgcgcgcgag atcacgaacc tggggctgac ggtctacccg agcgtcggca
      901 acttcctcct catcggcttc ccgaccgagc cggggcggga cgcgacggcc gcgaacgcct
      961 acctggagcg ggagggcgtg atcccgcgca tgatgggctc ctacgggctg ccgcacgcgc
     1021 tcaggatcag cgtcggcctg gaagacgaga accggcgcgt ggtcgaggcg ctcggtttct
     1081 tcgtcggcaa cggctgagcg ggcggccgta gcgatggagc cgttccgccg cgtggcgatg
     1141 ctcggtatcg ggctgatcaa cggctcgttg gcgaaggtcc tcaagcgcga gggtgccgcc
     1201 ggcgagatcg tcgcctgcag tcgccgcgcc gagacgcgcg accgcgcgct ggaactcggc
     1261 ctgtgcgacc gcgccgtcgc cgacccggcg gatgcggtgc gtggcgccga cctaatcgtg
     1321 ctgggcacgc cgccgggggc cctgagcgca ctcgccgcgg cgatgtcccc ggggctcgac
     1381 cgggacgcga tcgtcaccga cgtgagttcg atcaaggccc aggtcgtgcg cgacgtggcc
     1441 ccgcaccttc ccgacccgtc ccgcttcgtc cccggccacc ccgtggccgg caccgagcat
     1501 tccggccccg acgccgcctt cgacacgctg ttcgtccgcc gccgctgcat cctcaccccg
     1561 acggaggaga ccgacccgca agccgtcgac cgcgtgcgcg cgatgtggga gctggccggc
     1621 tccgcggtcg acgtgatgac gcccgagcac cacgaccggg tgctcgccat cacctcgcac
     1681 ctgccgcacc tgatcgccta caccatcgtc
//
LOCUS       DASWNJ010001467         2596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_309733, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001467 DASWNJ010000000
VERSION     DASWNJ010001467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309733"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            346..1116
                     /locus_tag="VGB85_24385"
     CDS             346..1116
                     /locus_tag="VGB85_24385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEY0137253.1"
                     /translation="MSVTRNDQGEQATGGERDPRPLVLIPTYNERENLAAIVAATLEA
                     LPAARVLVIDDASPDGTGALADALAQADARVAVLHRAGKQGLGRAYVDGFRRGLATTP
                     APTHFISMDADFSHDPGYLAGLVEACHPGPGGADVVIGSRYVRGGGTRDWSFARRMIS
                     RAGGLYARAVLGLPVRDPTAGFVCYARHVIADLDLAAVSASGYAFQIEMKHRAHRRGF
                     VLRERPIVFPDRTRGASKMTPAIAAEAVTLVWRLRGIA"
     gene            1153..2043
                     /locus_tag="VGB85_24390"
     CDS             1153..2043
                     /locus_tag="VGB85_24390"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEY0137254.1"
                     /translation="MPTKVYLSTAGRPLDPAEATVSVFDRGFLYGDSVYETMRTAGGH
                     AVEMTRHLDRLRRSAAGIGLEIPFADAELRAAIAETHAASGNPDSYVRVIVTRGAGPL
                     QLDPRVVASPLLVVLVQELKLPAPALYETGLSVRIVDIHKTSARSLDPTLKTGNYLNS
                     IQALRQAAESAAEDAIMCNAAGHVAEGATSNVFMVKDGRVVTPSLATGLLEGITRALV
                     MRLAADLAMPCGEGVVWPDELRAADELFLTSSVRGLMPVTTLDGKPVGAGPGPVTRRL
                     MARYQAYIDAVAAGGPELLT"
     gene            complement(2048..>2596)
                     /locus_tag="VGB85_24395"
     CDS             complement(2048..>2596)
                     /locus_tag="VGB85_24395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137255.1"
                     /translation="DVIVAGHIYVPIGLSDLWLRKYSAAGKEMWTRTADHMKGNDEWR
                     GVAVAPTGELALTGEVYEVAGLAAIYTAKYSSAGFETWAQIQDSAGGDNDRGHGVAFD
                     LAGNIVAVGSEYTANDFTRTWTRKHDPAGQELWTQIHDGDGAGNDVAYAVAIDAVGNV
                     YAAGSEYVAGQFATVWLSKYAP"
BASE COUNT          342 a          908 c          984 g          362 t
ORIGIN      
        1 catcagcttg ccgggcaggc cggggaaata gacctcgcac agcacctcct cgccggggtg
       61 gaagcgctcg gtggtgtaga cctgcaggcc ctgctcctcg ccaggcagca gggccgcgac
      121 gaaggccgac ccggactcgt agtgacagcg gatccttcgc aacgctcacg gcgagggtag
      181 cagcaggggg ctcggggtcg caagcgcgga ggggcgcgca gggggcccgc gggcgccccg
      241 gggaggtccg cgagtccccg gcatgccccg ggaggcgccg ggtcccgcgg aggtccgatc
      301 gcgcgccctt gcggcgcggc ggggctgctg ccaaggttcc gccgcgtgtc cgtgacgagg
      361 aacgatcaag gggagcaggc gaccgggggc gaacgagatc cccgtccgct ggtccttatc
      421 ccgacataca acgaacgcga gaacctcgcg gcgatcgtcg ccgcgacgct cgaggcgctg
      481 ccggcggcgc gggtgctggt gatcgacgac gcgagcccgg acggcaccgg cgcgctggcg
      541 gacgcgctcg cgcaggcgga cgcgcgggtg gcggtgctgc accgcgcggg caagcagggg
      601 ctcgggcgcg cctacgtcga cggctttcgc cgcggcctcg cgaccacgcc ggcgccgacg
      661 cacttcatca gcatggacgc cgacttctcg cacgaccccg ggtacctcgc cgggctggtc
      721 gaggcctgcc atccagggcc gggcggggcc gacgtcgtga tcggctcgcg ctacgtgcga
      781 ggcgggggca cgcgcgactg gtcgttcgcg cggcgcatga tctcgcgggc gggcgggctg
      841 tacgcgcggg cggtgctcgg cctgccggtc cgtgatccga cggcgggctt cgtctgctac
      901 gcgcgccacg tgatcgccga cctcgacctc gcggcggtgt ccgccagcgg ctacgcgttc
      961 cagatcgaga tgaagcaccg cgcgcatcgc cgcggcttcg tgctgcgcga gcggccgatc
     1021 gtgttccccg accgcacgcg cggcgcctcc aagatgaccc cggcgatcgc cgccgaggcc
     1081 gtgacgctgg tgtggcgcct gcgtgggatc gcctgacatc agccgtcatc gcccgaacca
     1141 ggagcagcga gcttgccgac caaagtctat ctgtccaccg ccggccgtcc cctcgatccc
     1201 gccgaggcga ccgtcagcgt gttcgaccgc gggtttttgt acggcgacag cgtctacgag
     1261 acgatgcgca ccgccggcgg ccacgcggtc gagatgacgc gccacctcga tcgcctgcgc
     1321 cgcagcgccg ccggcatcgg cctcgagatc ccgttcgcgg acgcggagct gcgcgcggcg
     1381 atcgccgaga cgcacgcagc gagcggcaac ccggacagct acgtgcgcgt gatcgtgacg
     1441 cgcggcgcgg ggccgctgca gctcgacccg cgcgtggtcg cgtcgccctt gctggtcgtg
     1501 ctggtccagg agctcaagct cccggcgccg gcgctgtacg agaccgggct gtcggtgcgc
     1561 atcgtcgaca tccacaagac cagcgcgcgc tcgctcgacc cgacgctcaa gaccggcaac
     1621 tatctcaaca gcatccaggc gctgcgccag gcggccgaga gcgcggcgga ggacgcgatc
     1681 atgtgcaacg ccgcggggca cgtcgccgag ggcgcgacga gcaacgtgtt catggtcaag
     1741 gacggccggg tggtgacgcc gtcgctggcg accgggctgc tcgagggcat cacgcgcgcg
     1801 ctggtgatgc gcctcgccgc ggacctggcg atgccctgcg gcgagggcgt cgtgtggccg
     1861 gacgagctgc gcgccgccga cgagctgttc ttgacgtcgt cggtgcgcgg cctgatgccg
     1921 gtgacgacgc tcgacgggaa gccggtcggc gcggggccgg gcccggtcac gcggcggctg
     1981 atggcgcgct atcaggcgta catcgacgcg gtcgcggccg gcggccccga gctgctgacc
     2041 tgagtgttca cggcgcgtac ttgctgagcc acacggtcgc gaactggccg gcgacgtact
     2101 cgctgccggc ggcgtagacg ttgccgaccg cgtcgatcgc gacggcgtag gcgacgtcgt
     2161 tgccagcccc gtcgccgtcg tggatctgcg tccacagctc ctggcccgcg gggtcgtgct
     2221 tgcgggtcca ggtgcgggtg aagtcgttgg cggtgtactc cgagccgacg gcgacgatgt
     2281 tgccggcgag gtcgaaggcg acgccgtggc cgcggtcgtt gtcgccgccg gcgctgtcct
     2341 ggatctgcgc ccaggtctcg aagccggcgc tgctgtactt cgcggtgtag atcgcggcga
     2401 ggccggcgac ctcgtagacc tcgccggtca gcgcgagctc gccggtcggc gcgacggcga
     2461 cgccgcgcca ctcgtcgttg cccttcatgt gatcggcggt gcgggtccac atctccttgc
     2521 cggcggccga gtacttgcgc agccacaggt cggagaggcc gatcgggacg tagatgtggc
     2581 cggcgacgat cacgtc
//
LOCUS       DASWNJ010001468         2413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_237961, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001468 DASWNJ010000000
VERSION     DASWNJ010001468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237961"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VGB85_24400"
     CDS             <1..305
                     /locus_tag="VGB85_24400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137256.1"
                     /translation="VPLRRLWEAVGATDQKPLLAPGDRSEAGRAALATELRLEPSEAA
                     WLAQFDGTRHLGAMLDDFDADEHELLSLVYTLKMIRRLDLVTPEPIPFARQRGSEF"
     gene            complement(390..971)
                     /locus_tag="VGB85_24405"
     CDS             complement(390..971)
                     /locus_tag="VGB85_24405"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY0137257.1"
                     /translation="MSDPTPTLARGLAFALAAHGAQTRKGTEIPYVSHLLQVSGLVLE
                     YGGDMIQATAALLHDVVEDTPATLAEVTAAFGAEVAAIVADCTDTQGDEAPTHKRPWL
                     ERKQGYVARLAEVPHRSALVVGCDKLHNMRTLVTSLENEGPGVLARFNAPPVQQRWLH
                     AALLAALRGKLPEALYHELQGLTGRYAGLTAAT"
     gene            1190..1687
                     /locus_tag="VGB85_24410"
                     /pseudo
     CDS             1190..1687
                     /locus_tag="VGB85_24410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969314.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
     gene            1867..>2413
                     /locus_tag="VGB85_24415"
     CDS             1867..>2413
                     /locus_tag="VGB85_24415"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY0137258.1"
                     /translation="MQRYDDEAGPAQASAAPQFPATMLALEWHGNKRVEVAARPRPAI
                     AEVQDAIIRVTSTSICGSDLHIYHNAVPGMHSGDVLGHECVGIVEEVGPQVQTLRPGD
                     RVVVSSVIAEGHCHYCREGLFALCEVTNPVEGGQQLYGHCSAGMVGYSHLTGRHDGAQ
                     ASHIRIPYADVNCLVVPAGLTD"
BASE COUNT          377 a          923 c          767 g          346 t
ORIGIN      
        1 gcgtcccgct gcgccgcctg tgggaggccg tcggcgccac cgaccagaag cccctgctcg
       61 cccccgggga ccgcagcgag gccggccgcg ccgcgctggc caccgagctc cggctcgagc
      121 cgagcgaggc cgcctggctg gcccagttcg acggcacgcg ccacctcggc gcgatgctcg
      181 acgacttcga cgccgacgag cacgagctgc tctcgctcgt ctacaccctg aagatgatcc
      241 gccgcctcga cctcgtcacc cccgagccga tccccttcgc ccgccagcgc ggctccgaat
      301 tctaggcgcg acaccacgtt cgggcctcgt gctgccgcgc cggggatatg ttcacctggt
      361 tcgcgcgtgg acgatgatat ccggcgcgct caggtggcgg cggtcaaccc ggcataacgc
      421 ccggtcaacc cctgcaactc atgatataac gcctccggca gcttcccgcg cagcgccgcg
      481 agcagcgccg cgtgcagcca gcgctgctgc accggcggcg cgttgaaccg cgccagcacc
      541 cccggcccct cgttctcgag cgaggtcacc agcgtgcgca tattgtgcaa tttgtcgcac
      601 cctacaacca aggcgctgcg gtgaggcacc tccgcgagcc gcgcgacata cccttgtttg
      661 cgctcgagcc acggccgctt gtgcgtcggc gcctcgtctc cctgcgtatc ggtgcaatcc
      721 gcgacgatcg cggccacctc ggcgccgaac gccgccgtca cctccgcgag cgtcgccggc
      781 gtgtcctcga cgacgtcgtg cagcagcgcc gccgtcgcct ggatcatgtc gccgccgtac
      841 tcgagcacca ggccgctgac ctgcagcagg tggctcacgt aggggatctc ggtgcccttg
      901 cgcgtctggg cgccgtgggc ggcgagcgcg aaggcgaggc cccgcgcgag ggttggcgtg
      961 ggatccgaca cgtcctcaat ctaccagtcc gctcgacacg cgtaacgggt cttcctatcc
     1021 tgtccccatg ctccgacccc tcgccgccct cgtcgtcctg tccctcgccg cctgcgactc
     1081 ctccgcgaag accgagcccg cgaagaccga gcctgccagc cccgagtcga acaagaccga
     1141 gcccgccagc cccgagtcga acaagaccga gcccgcgcag ctcgccgacg ccagcggccc
     1201 gatcctcgat cacgaggtcg agaccctcga gggcacgaag cagaagctca gtgattatcg
     1261 cggcaaggcc gtgctgctcg tcaacgtcgc ctccgagtgc ggctacacgc cgcagtacgc
     1321 ccagctgcag gagctctacg gccgttacaa ggatcgcggg ctcgtcgtgc tcggcttccc
     1381 gtcgaacgac ttcggcggtc aggagcccgg cgacgccaag cagatcaagg agttcgtcac
     1441 cagcaagttc gcggtcgact tcccgatgat ggccaaggtg cacgccaccg gccccgagaa
     1501 ggcgcccctc tacaagacgc tgaccgagga gacccccgag agcatgcgcg gcgaggtgaa
     1561 gtggaacttc accaagttcc tcgtcgaccc gaccggcaag gtcgtcgcgc gcttcgagtc
     1621 caagatcgag ccgacctccc ccgagctgat cggcgcggtc gagaaggtcc tccccgccaa
     1681 gtcctgagcc gggtcggccg aaccgtcgag cactgccaca acccgcaccg ggctatccct
     1741 cagcgcccgg tgcgcagcca cgcgccccgg cccgtgcagc gtgcatcgag gccctcttgt
     1801 ggccggtgcg agcgcgtcga tgcctaccct gcggctccgc gacgtaggtc tatggcgagg
     1861 gagaccatgc aacgatacga cgacgaggcc gggccggcgc aggcgagcgc cgcgccgcag
     1921 ttcccagcga cgatgctcgc gctcgagtgg cacggcaaca agcgggtcga ggtcgccgcg
     1981 cgcccgcggc cggcgatcgc cgaggtccag gacgcgatca tccgcgtcac cagcacgagc
     2041 atctgcgggt ccgacctgca catctatcac aatgccgtcc ccggcatgca cagcggcgac
     2101 gtgctcggcc acgagtgcgt gggtatcgtc gaggaggtcg gcccgcaggt ccagaccctg
     2161 cgccccggcg accgcgtcgt cgtctcctcc gtgatcgccg aggggcactg ccactattgc
     2221 cgcgagggcc tgttcgccct gtgcgaggtc accaatccgg tcgagggcgg gcagcagttg
     2281 tatggccatt gcagcgcggg catggtcggc tattcgcacc tgaccggccg ccacgacggc
     2341 gcccaggcca gccacattcg catcccctac gccgacgtca actgcctcgt cgtgcccgcg
     2401 ggcctcaccg acg
//
LOCUS       DASWNJ010001469         2634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_485440, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001469 DASWNJ010000000
VERSION     DASWNJ010001469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485440"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..1917
                     /locus_tag="VGB85_24420"
     CDS             76..1917
                     /locus_tag="VGB85_24420"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0137259.1"
                     /translation="MVPRHETAALVAGARRQLGDHPDAVWLHAQVLAMQSDESGLAAL
                     AAIPGLPDSVRDFVHAKQVVLTFFRFEAAPPPMRPSLEQLQELVARAERTAPSGTLAV
                     ARAAEGWLSLGRVDLAVELLERAQARRPDALAFTVLRAQARVRQDASLRDAVVGDLTA
                     LADEYRADPDALARLVAGLLSLEAAEAARAIQDDVRRRLPGARVVERRRVPGARVVER
                     WDFDAASVFGAVGDCRLARAKGTLPAADKLAQARAPVDAFFDRPGIRDPSLRAATAAA
                     LVEFLQCEPEAPLQRVEAVARAVLEAPFVRDDAMASVLVASRGGDLELARALAERAVA
                     LARETPFEFEIVLTPDEHEQNRRAAQAYAHHARGLVHLRAARLDEARADISRALELRP
                     TQPDIALLAAELAERDGDLRRAEQTLARAIVREGAWARACQDRLLELMHARGGKEVDL
                     AGTIARLDREWKDERIAEVLAARAAAPKPLAPFELDLRGGGRITSDSLLGRVTVVIAT
                     EPWCTACKLEAPELAKLQRRYHGRKDVQFLVVTDDPDGIAALHEGAGFHTTIAKDDGW
                     RRTVGINSYPTHLFIDAGGREVFRESGDSNEMHFTYPALIEALRRER"
BASE COUNT          360 a          921 c          960 g          393 t
ORIGIN      
        1 ctcgcggtgc accgcgcggc gctccccgac ggatccgggt gggcgctcct gctgcacgcg
       61 caggagcgct cgagcatggt cccgcggcac gagacggccg cgctggtcgc gggcgcccgc
      121 agacagctcg gcgatcaccc cgacgcggtg tggctgcatg cgcaggtgct ggcgatgcag
      181 agcgacgaga gcgggctcgc tgcgctcgcg gcgatccccg gtctgccgga cagcgtgcgg
      241 gacttcgtgc acgcgaagca ggtcgtactc acgttcttcc gcttcgaggc cgcgccgccg
      301 ccgatgcgac cgagcctcga gcaactgcag gagctcgtgg cccgcgccga gcggaccgcg
      361 ccctcgggga cgctcgccgt tgccagagcg gccgagggat ggctaagtct cggccgggtc
      421 gacctggcgg tcgagctcct tgagcgggcg caagcgcggc gccccgacgc gctggccttc
      481 accgttctgc gcgcacaagc tcgcgtgcgt caggacgcct cgctgcgcga cgcggtggtc
      541 ggcgacctca cggcgctcgc cgacgagtac cgcgccgatc cggatgcgct cgcgcggctc
      601 gtcgccggcc tgttgtcgct cgaggcggcc gaggccgcgc gagccatcca ggacgacgtg
      661 cgccggcgcc tccccggtgc gcgtgtggtc gaacgccggc gcgtccccgg tgcgcgtgtg
      721 gtcgagcgct gggactttga cgcggcgtcg gtgttcggcg ccgtggggga ctgcaggctg
      781 gcgagggcga agggcacgct gcccgcggcg gacaagctgg cgcaggcgcg cgcgcccgtc
      841 gacgccttct tcgatcggcc ggggatccgc gacccgtcgc tgcgcgcggc gacggcggcc
      901 gcgctcgtcg agtttctgca gtgcgagccg gaagcgccgc tgcagcgagt cgaggcggtg
      961 gcgcgcgccg tgctcgaggc gcccttcgtg cgggacgacg ccatggcgtc ggtgctcgtg
     1021 gcgagccgtg gcggcgacct cgagctcgcg cgtgcactcg ccgagcgcgc ggtcgcgctc
     1081 gcacgcgaga cgccatttga gttcgagatt gttcttacac ccgacgagca cgagcaaaac
     1141 agacgggccg cgcaggcgta cgcccaccac gcccgcgggc tcgtgcacct gcgcgcggca
     1201 cggctggacg aggcgcgggc cgacatctca cgtgcactcg agctccgtcc gacgcagccg
     1261 gacatcgcgc tgctcgcggc cgaactggcc gagcgcgacg gcgacctccg ccgcgccgag
     1321 cagaccctcg cgcgcgcgat cgtaagggag ggcgcgtggg cacgggcgtg ccaggaccgg
     1381 ttgctcgagc tcatgcacgc acgcggcggg aaagaggtcg atctcgctgg cacgatcgcg
     1441 cggctcgacc gcgagtggaa ggacgaacgg atcgcagagg tgctcgccgc gcgggccgct
     1501 gcgccgaagc ccctcgcgcc gtttgagctc gacctgcgcg ggggcggccg gatcacgagc
     1561 gactcgctgc ttgggcgcgt caccgtggtg atcgcgaccg agccgtggtg caccgcatgc
     1621 aagctcgagg cgcctgagct ggcgaagctg cagcgacgct accacggacg caaggacgtg
     1681 cagttcctgg tcgtgaccga cgatcccgac gggatcgcgg cgctgcacga gggggcggga
     1741 ttccacacga cgatcgcaaa ggacgatggc tggcggcgta cggtcggcat caacagctat
     1801 ccgacccacc tgttcatcga tgccggcggc cgcgaggtct tccgtgaatc cggcgactcc
     1861 aacgagatgc actttactta cccggcgctt atcgaggcgc tgaggcgcga gcggtagaat
     1921 cggctccact gcagcgttcg ggccctgtac cgcctgcttg cgaaaggcgg cgagacgaca
     1981 cgctggcgag gcgaggttcg tggtcgaggc cggcgaggag gcgtgacggc tcttcgacct
     2041 cgccttgctg cgtcgtcgtc tcgcgtcttt cccgtcagca cgcgctcact cagtgctaac
     2101 gccatttcaa gttgttcaag gcgcggccgt ccgcggaaca gtccgcggcc tcgatcccaa
     2161 gggtcgtcgt gcgcgcgcgt ggtcgatcgc ggagcgcctc gccccgggga gggccaccac
     2221 ggggtgcgca agtgcgcgcg ggcctgaccc aagtccgcgc aggttgctaa ccgcgcggat
     2281 tcgcgctggt tcttggcaaa tgtgcgcgcc tgcaggttct cacatctgcg cagggctgtg
     2341 ccggtatcga gatcgaaggc atcggcccgc gagcatccgg tcccgcgcct gatacgccga
     2401 ggtcatgcgg cgtcatgccg cgtcatcgca tcgcaccgtg tcatgcactc gttcgtggct
     2461 ccgactgata cagtgcgagc gtatggtcgg atcgatgcgt gcacccgtct cgctattctc
     2521 ctgcggtctc ctgctctcgg cctgcccggg cgacctcaca gcggacagcg tcacggccac
     2581 agtcggcgtc gcttcgctga ccgacacgcc ttcgaccgac accgtcgtcg cgcc
//
LOCUS       DASWNJ010001470         6880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_26703, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001470 DASWNJ010000000
VERSION     DASWNJ010001470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6880
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26703"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(296..1594)
                     /locus_tag="VGB85_24425"
     CDS             complement(296..1594)
                     /locus_tag="VGB85_24425"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEY0137260.1"
                     /translation="MPFTAWYPALGALFIVMALAGSAVKRLPISPAMLYLAAGLLLGP
                     RVAGLVDLDLADDAMLLGRLGEVAVLVSLFTTGLKLRVPLRDRLWRAPIRLASVSMIF
                     TVAAVALVGMLLLGLSAGAAVMLGAVLAPTDPVLASDVQVEDPRDTETVRFGLTGEAG
                     LNDGSALPFLMLGLGMLGLHELGAWGWRWLAVDVVWSIGAGLGVGVLCGAAVARLVLY
                     LRRHHQEALGLDEFLALGLIALSYGLAHLIHSYGFLAVFAAGVALGRTESESSDAPPP
                     AVDAAAHASDAEVDEPASEPMTQAVLTFNEQFERIAEVALVLCVGAMLPQVSWPEHGA
                     TFLAVLLLVIRPLAVWLGLRGAGLSGVQVAFMSWFGVRGIGSLYYVAFALVLGFSGGE
                     AQLLVDLALIVIATSIVLHGISVTPLMRRYRKTPECSDPA"
     gene            complement(1614..2750)
                     /locus_tag="VGB85_24430"
     CDS             complement(1614..2750)
                     /locus_tag="VGB85_24430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine pyrophosphorylase"
                     /protein_id="HEY0137261.1"
                     /translation="MTDLAFMAAVQERRLADLVARGVHVIDPRQTYVDAGVLAERIHP
                     GVVLHPGTRLIGQRTFLGPEAELGREGPVTAIDCVLGEGARIDGGYARDAVLLRGASA
                     GSAAHLREGTLLEEEASTAHAVGLKHTILMSFVTVGSLVNFCDLLMAGGSSRKDHSEV
                     GSGYIHFNFTPWGERGDKATPSLIGDVPRGALLRARRIFLGGSGGMVGPRVVGFGAVA
                     GAGQVLRKDVPEDRMVIQLPRELERPFPYDAIDPPEPRAIHNARFIGELTALRAWYSE
                     IRLRRAPETGPFEHVRTVTQEAIASIDLAIAERQIRFSAFLRERGVDAPALRPSRPHP
                     CPLVLSPDSGSYTDHVEWVKGLPDADIAALSSWLAGIVRDVANS"
     gene            2819..3445
                     /locus_tag="VGB85_24435"
     CDS             2819..3445
                     /locus_tag="VGB85_24435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137262.1"
                     /translation="MDERAKLTDQELREAAAEAGISPEELRQALVQRSGGGPGNALAK
                     SAGTAVAGYSAQTRLALPPDRAIEGVRTAIARQVGHRGHRQGNGDIDILDDRTNMLYR
                     IHADPDGQGGALVAVRSERTGSSLALAAVMFTTVALGVTALGFLLSSLLMWLGLGLLV
                     AGGAGVFLAGGRAAGMQRQAELVVAQALVEAEEAVPVGPEVVRALPPE"
     gene            3467..4000
                     /gene="greB"
                     /locus_tag="VGB85_24440"
     CDS             3467..4000
                     /gene="greB"
                     /locus_tag="VGB85_24440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEY0137263.1"
                     /translation="MAEETPKFFITAEGLRNLRAELDHLWKVERPRVTAEVGAAAALG
                     DRSENAEYIYGKRRLREIDRRLEFLAKRLDNLKVFHPPAKEPDKVTFGAWVTLEDEDG
                     ATVHYQLVGADEFDVALGKISVASPVGKALLGKEEGDDVVVERPRGRTVFTIMAISYK
                     RPAAAPAPSQQPENPEN"
     gene            4001..4834
                     /locus_tag="VGB85_24445"
     CDS             4001..4834
                     /locus_tag="VGB85_24445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0137264.1"
                     /translation="MTTSNPSLHGKTIFISGASRGIGLAIGLRAARDGANVVVAAKTT
                     EPHPKLPGTIYSAAQEIEAAGGKALPLMVDIREEAQVLAAVAKTVETFGGIDILINNA
                     SAISLTGTLETPMKRYDLMHTINTRGTYLCSQACIPHLKQAKNPHILNLSPPLNMKAH
                     WFKNHVAYTMAKYGMSMCVLGMSAEFEDEGIAVNALWPRTAIATAAINMIGGPAMMAG
                     CRTVDIMADAAHLILTRDSRTCTGNFFLDEDLLREAGTTDFERYSVTPGATLIPDFFL
                     D"
     gene            4844..6241
                     /locus_tag="VGB85_24450"
     CDS             4844..6241
                     /locus_tag="VGB85_24450"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEY0137265.1"
                     /translation="MNPIILGVALYVLAQLALGLWVARRVSSEEDYLLAGRSLGYGLA
                     TFTIFATWFGAETCVGAAGQVFEHGLAGGASEPFGYALCLLLMGTVFAAALWRKGYVT
                     VADVVRARFGVHAERFAVLLVAPTSVMWAAAQIRAFGQVLAATAGVDADAAVAVAAAI
                     VLFYTVSGGLLADAITDLVQGLALIVGLVVLLVAVVSHLGGVGPALAAIDPSRLSFGS
                     SDLGALDILDSWAIPVCGSLVAQELVARVLACKSATVARRATIAGGLVYLAVGLIPVF
                     IGLVGVTLIPNLAQPEQIVGEVARLHLGTLGYIAFVGALISAILSTVDSALLAAASLT
                     AHNLVVPLRPGITDAGKLRVSRMCVFVFGVIAYLLARGAERVHDLVEDASAFGTGGLF
                     VVFVMAMCTRLGGPRSAIAALAAGLVVWLGGRHLVEIPAPFLCSVAAAALAFVLVAVL
                     EARFTRPGRPAPAAP"
     gene            complement(6204..>6880)
                     /locus_tag="VGB85_24455"
     CDS             complement(6204..>6880)
                     /locus_tag="VGB85_24455"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0137266.1"
                     /translation="YREQMLAATAAGYTELKQLIAERRERPGEDLLSTLIHLEEEGTR
                     VHESELLGMVGAIVIAGSDTTVHTMRFLLLDLLQNPEVLERVRRDPGLVRSAMEESLR
                     FNNFNRLSAPLYALEDANLHGVEVAKGQTVFTLTAAAGRDPAVFVHPDRFDIDRPDLA
                     EARSFGVGPHTCLGIHLARLETEVAIPLILERFPTMTLAGPPVYGQNAFFRAIKELPV
                     RVVPAA"
BASE COUNT         1045 a         2435 c         2436 g          964 t
ORIGIN      
        1 tcgaggtcga gcaggccggc gaagcgcggg ccgagcagca caccggcgcc gaggtacaac
       61 atcgccgggc taaacggcag tcgcttcacc gacgagcccg ccagcgccat cacgatgaac
      121 agcgccccga ggacggggta ccacgcgctg accggcaccc acgaaataga gcacctgcgc
      181 cggtgctggc cacgccgtca ctggcggcgc gggccagggg gccggcgcga ggccagcggt
      241 gcgctcgctg ctccgtccgg cgctggcatc gctggcatgc gctgaaacga gcgcgtcacg
      301 ccgggtcact gcactcgggg gtctttcgat agcgtcgcat cagcggcgtc accgagatcc
      361 cgtgcagcac gatcgacgtg gcgatgacga tcagggccag gtcgacgagc agctgcgcct
      421 cgccgccgga gaagccgagg acgagggcga aggcgacgta gtagagcgaa ccgatgccgc
      481 gcacgccgaa ccagctcatg aacgcgacct gcacgcccga cagcccggcg ccgcgcagac
      541 cgagccacac cgcgagcggg cggatcacca gcagcagcac cgccaggaac gtggcgccgt
      601 gctccggcca cgacacctgc ggcagcatcg cgccgacgca cagcaccagc gcgacctcgg
      661 cgatgcgctc gaactgctcg ttgaaggtca gcaccgcctg cgtcatcggc tcgctcgctg
      721 gttcgtcgac ctccgcgtcg gaggcgtgcg ccgccgcgtc gaccgcgggc ggcggcgcgt
      781 cgctcgactc gctctccgtc cgtccgagcg cgacgccggc ggcgaacacc gcgaggaagc
      841 cgtagctgtg gatcaggtgc gccagcccat acgacagcgc gatcaggccg agcgcgagga
      901 actcgtcgag gccgagcgcc tcctggtgat gccggcgcag gtagagcacg aggcgcgcca
      961 ccgcggcgcc acacagcacg ccgaccccga ggcccgcgcc gatgctccac acgacgtcga
     1021 cggcgagcca gcgccagccc cacgcgccga gctcgtgcag gccgagcatg ccgaggccga
     1081 gcatcaggaa cggcagggcg ctgccgtcgt tgaggccggc ctcgccggtg agtccgaagc
     1141 gcacggtctc ggtgtcacgc gggtcctcga cctggacatc ggaggcgagc acggggtcgg
     1201 tcggcgcgag cacggccccg agcatcacgg cggcgccggc cgagaggccg agcagcagca
     1261 tgccgacgag cgcgacggcg gcgacggtga agatcatcga gacggacgcg aggcggatcg
     1321 gtgcgcgcca caggcggtcg cgcagcggca cgcgcagctt gagcccggtg gtgaacagcg
     1381 agacgagcac cgcgacctcg ccgaggcgcc cgagcagcat cgcgtcgtcg gcgaggtcga
     1441 ggtcgacgag cccggcgacg cgcgggccga gcagcagacc cgccgcgagg tacaacatcg
     1501 ccgggctgat cggcagccgc ttcacggcgg agcccgcgag cgccatgacg atgaacagcg
     1561 ctccgagcgc ggggtaccac gcggtgaacg gcacccgttt atgaaagcac gtgctagctg
     1621 ttggccacgt cgcggacgat gccggcgagc cacgagctca gcgccgcgat gtcggcgtca
     1681 ggcaggccct tcacccattc gacgtggtct gtgtacgatc cagagtcggg gctgaggacc
     1741 agcgggcacg ggtgcggccg gctgggcctc agcgcaggtg cgtcgactcc gcgctcgcgc
     1801 aaaaacgcgc taaaccggat ctggcgttcc gcgatcgcca gatcgatcga cgcgatcgcc
     1861 tcctgggtga ccgttcggac atgttcgaaa ggacctgtct ccggggcccg gcgcaggcgg
     1921 atctccgagt accaggcgcg cagggccgtc agctcgccga tgaagcgggc gttgtggatc
     1981 gcccgcggct cgggcgggtc gatcgcgtcg taggggaacg ggcgctcgag ctcgcggggc
     2041 agctggatca ccatcctgtc ctcggggaca tctttgcgca gcacctggcc ggcgccggcg
     2101 accgccccga agccgacgac gcgcgggccg accatcccgc cgctgccgcc gaggaagatc
     2161 ctccgcgccc gcagcagcgc gccgcggggc acgtcgccga tcagcgaggg cgtcgccttg
     2221 tcgccgcgct cgccccacgg cgtgaagttg aagtggatgt agccgctgcc gacctccgag
     2281 tggtccttgc gcgagctgcc gccggccatc agcaggtcgc agaaattgac gagcgagccg
     2341 accgtcacga acgacatcag gatcgtgtgc ttgaggccga ccgcgtgggc ggtgctcgcc
     2401 tcctcctcga gcaaggtgcc ctcgcgcaga tgtgcggccg agccggccga ggcgccgcgc
     2461 agcagcacgg cgtcgcgggc gtagccgccg tcgatgcggg cgccctcgcc gagcacgcag
     2521 tcgatcgcgg tgaccggccc ctcgcggccg agctcggcct cgggcccgag gaaggtccgc
     2581 tggccgatca ggcgcgtgcc cgggtgcagc accacccccg ggtggatccg ctccgcgagg
     2641 accccggcgt cgacgtaggt ctggcgcgga tcgatgacat ggaccccgcg cgcgaccagg
     2701 tccgcgagtc ggcgctcctg cacggccgcc atgaaggcaa gatcggtcat ttcgcgatgt
     2761 taacatgggc ccccggggct ccacgcggcg cggcgattac gctactgtcg gtgccgcgat
     2821 ggacgagcgg gccaaactca cggatcagga gctacgcgag gccgcggccg aggccggcat
     2881 ctcccccgag gagctgcgtc aggcgctggt ccagaggtcc ggtggtggcc cagggaacgc
     2941 gctcgccaag tccgcgggta ccgccgtggc cggctactcc gcgcagaccc gactcgcgct
     3001 cccacctgac cgcgcgatcg aaggcgtacg caccgcgatc gcccgacagg tcggtcatcg
     3061 cggacaccgg cagggcaacg gcgacatcga catcctcgat gatcgcacca acatgctcta
     3121 ccgcattcac gccgaccccg acgggcaagg cggcgccctc gtggcggtcc gcagcgaacg
     3181 gaccggctcg agcctcgccc tcgccgcggt catgttcacc accgtcgccc tcggggtcac
     3241 cgcgctcggc ttcctgctgt catcgctgtt gatgtggctc ggcctcgggc tgctggtcgc
     3301 cggtggggcc ggcgtgttcc tcgcgggggg ccgcgcggcg ggcatgcagc ggcaggccga
     3361 gctggtggtc gcgcaggcgc tggtcgaggc cgaggaggcc gtgccggtcg gccccgaggt
     3421 cgtacgagcg ctcccgccgg agtgagcacc tggtatgttc gccgcgatgg cggaggagac
     3481 cccgaagttc ttcatcacgg ccgaggggct gaggaacctg cgcgccgagc tcgaccacct
     3541 gtggaaggtc gagcgcccgc gggtgaccgc ggaggtcggc gcggcggctg ctctcgggga
     3601 caggtcggag aacgccgagt acatctacgg caagcgacgt ctgcgcgaga tcgacaggcg
     3661 cctcgagttt ttggcgaagc ggctcgacaa cctcaaggtg ttccatccgc cggcgaagga
     3721 gcccgacaag gtgaccttcg gcgcgtgggt cacgctcgag gacgaggacg gcgcgacggt
     3781 gcactaccag ctcgtcggcg ccgacgagtt cgacgtcgcg ctcggcaaga tcagcgtcgc
     3841 ctcgccggtc ggcaaggcgc tgctcggcaa ggaggagggc gacgacgtcg tcgtcgagcg
     3901 cccccgcggc cgcacggttt tcacgatcat ggcgatcagc tacaagcggc cggcggccgc
     3961 tcccgcaccc tcgcagcagc ccgagaaccc cgagaactaa atgaccacca gcaacccgag
     4021 tctccacggc aagaccatct tcatcagcgg agcgagccgc ggcatcggcc tcgcgatcgg
     4081 ccttcgtgcc gcgcgcgacg gcgccaacgt cgtcgtcgcc gccaagacca ccgagcccca
     4141 ccccaagctg cccggcacga tctactcggc cgcgcaggag atcgaggcgg ccggcggcaa
     4201 ggcgctgccg ctgatggtcg acatccgcga ggaggcccag gtgctcgcgg cggtggcgaa
     4261 gaccgtcgag accttcgggg gcatcgacat cctgatcaac aacgccagcg cgatcagcct
     4321 caccggcacg ctcgagacgc cgatgaagcg ctacgacctg atgcacacca tcaacacgcg
     4381 cggcacctac ctgtgctcgc aggcgtgcat cccccacctc aagcaggcca agaacccgca
     4441 catcctcaac ctctcgccgc cgctcaacat gaaggcgcac tggttcaaga accacgtcgc
     4501 ctacacgatg gcgaagtacg gcatgagcat gtgcgtgctc ggcatgtcgg ccgagttcga
     4561 ggacgagggc atcgccgtca atgctctgtg gccccgcacg gcgatcgcca ccgcggcgat
     4621 caacatgatc ggcggaccgg cgatgatggc cggctgccgc accgtcgaca tcatggccga
     4681 cgccgcccac ctgatcctca cgcgcgacag ccggacctgc accggcaact tcttcctcga
     4741 cgaggacctc ctgcgtgagg ccggcacgac ggacttcgag cgctactcgg tcaccccggg
     4801 cgcgacgctc atccccgact tcttcctcga ctgatcccgc ggcgtgaacc cgatcatcct
     4861 cggggtcgcg ctctacgtgc tcgcacagct cgcgctcggg ctgtgggtcg cgcggcgcgt
     4921 cagcagcgag gaggactacc tgctcgccgg ccgtagcctc ggctacggcc tcgccacgtt
     4981 cacgatcttc gccacgtggt tcggcgccga gacctgcgtc ggcgcggccg ggcaggtgtt
     5041 cgagcacggc ctcgccggcg gggcctccga gcccttcggc tacgcgctgt gcctgctgtt
     5101 gatgggcacg gtgttcgcgg cggcgctgtg gcgcaagggc tacgtgacgg tcgccgacgt
     5161 cgtgcgcgcg cgcttcggcg tccacgccga gcgcttcgcg gtgctgctcg tcgcgccgac
     5221 ctcggtgatg tgggcggccg cgcagatccg ggcctttggt caggtgctcg cggcgaccgc
     5281 gggggtcgat gcggatgcag cagtcgccgt cgcggcggcg atcgtgctgt tttatacggt
     5341 gtctggcggg ctgctcgccg acgcgatcac cgacctcgtg caggggctcg cgcttatagt
     5401 tgggctcgtc gtgctgctgg tggccgtcgt gagccacctc ggcggggtcg gccccgcgct
     5461 cgcggcgatc gatccatcgc ggctgtcctt tgggtcatcc gacctcggcg cgctcgacat
     5521 tttggacagc tgggcgatcc ccgtgtgcgg ctcactcgtc gcgcaggagc tggtcgcgcg
     5581 ggtgctcgcg tgcaagtcgg cgaccgtggc ccgccgcgcg acgatcgctg gcggcctcgt
     5641 gtacctcgcg gtcggcctga tccccgtgtt catcggcctc gtcggcgtga cgctgatccc
     5701 gaacctcgcc caaccggagc agatcgtcgg cgaggtcgca cgcctgcacc tcgggaccct
     5761 cggctacatc gccttcgtcg gcgcgctgat ctcggcgatc ctctcgaccg tcgacagcgc
     5821 cttgctcgcc gcggcctcgc tgacggccca caacctcgtg gtcccgctgc gaccgggcat
     5881 caccgacgcg ggcaagctcc gggtctcgcg gatgtgcgtg ttcgtgttcg gcgtgatcgc
     5941 ctacctgctg gcacgcggcg ccgagcgagt ccacgacctc gtcgaggacg cgtctgcgtt
     6001 cggcaccggc gggctgttcg tcgtgttcgt gatggcgatg tgcacgcgcc tcggcggccc
     6061 gcgcagcgcg atcgcggcgc tggccgcggg cctggtcgta tggctcggcg ggcgccacct
     6121 cgttgaaatt ccggcgccgt ttttgtgctc ggtcgccgcg gcggcgctgg ccttcgtgct
     6181 cgtggctgtc ctcgaggcca ggttcacgcg gccgggacga cccgcaccgg cagctccttg
     6241 atcgcgcgga agaacgcgtt ctggccgtac accggcggac cggcgagggt catcgtcggg
     6301 aagcgctcga ggatcagagg gatcgccacc tcggtctcga ggcgggcgag gtggatgccg
     6361 aggcaagtgt gcgggccgac gccgaagctc ctggcctcgg cgaggtccgg gcggtcgatg
     6421 tcgaagcgat cgggatgcac gaagaccgcc gggtcgcgcc cggccgcggc cgtcagggtg
     6481 aacactgtct gtcccttcgc aacctcgacg ccgtgcaggt tcgcgtcctc gagggcatac
     6541 aggggggcgc tgaggcggtt gaagttgttg aagcgcaggc tctcttccat cgcggagcgc
     6601 accagaccgg ggtcacgacg cacccgctcc agcacctccg ggttctgcag cagatccagc
     6661 agcaggaagc gcatcgtgtg cacggtggtg tccgaaccgg cgatgacgat cgcgccgacc
     6721 atgccgagca gctccgactc gtgcacacgc gtgccctcct cctcgagatg gatgagcgtc
     6781 gacagcaggt cctcgccggg acgctcgcga cgctcggcga tcaattgctt gagctcggtg
     6841 tagccggccg cggtggccgc cagcatctgc tcacgatagg
//
LOCUS       DASWNJ010001471         9191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_557698, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001471 DASWNJ010000000
VERSION     DASWNJ010001471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 9191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9191
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557698"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1317
                     /locus_tag="VGB85_24460"
     CDS             <1..1317
                     /locus_tag="VGB85_24460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0137267.1"
                     /translation="EQTLRSLALRAQERGLALDCTIAPEVPAYVVGDPDRLRQVLVNL
                     VGNAIKFTEKGRVTVRVRAAPGPGPRVVFEIEDTGIGIPPERLRAIFVPFEQADPSTT
                     RRFGGTGLGLTIASRLVGLMGGEIGVASEVGVGSTFTFDVLFSPVSSETPTRRAAPPP
                     LSTRAAGKRRLEILLAEDNPINQRVAVEILKRMGHTVTVVNDGRAAVEAVENGRFDLV
                     LMDVQMPIMDGWQATATIRRSPHAHARHTRVVALTARATDEDRKRCMAHGMDGFLTKP
                     LQVAELLHLCEEGKAPERAAAPVTARSPTPSTSSPSSTSSTSSTPSGERQSIDDWPGV
                     LQRVGGSPAMLARLVGMYRDELPAVLAELRATLISGEGLRRAAHRIAGTVGIFNARHA
                     LALAREVEERAPQRAPDLGARVEALCAELTRLDRELFTRSQAFGSG"
     gene            1460..2251
                     /gene="radC"
                     /locus_tag="VGB85_24465"
     CDS             1460..2251
                     /gene="radC"
                     /locus_tag="VGB85_24465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEY0137268.1"
                     /translation="MHENISYQAEYARRERVAGPPVGFVPEDDEIEVATIVPSAIERP
                     RERMFAFGPAALSNVELVALLLGGGRSEQRALALLQQVGGIGGLLRCVPGELLETPGV
                     GEASATAVCAAVELARRIGQIEMPFESPIQGPEDVRRYVRASLRGRAQEIFMVLGLDG
                     RQRVRLVREVAIGSLSHVEVHPREVFRPLVRAGSHSVILVHNHPSGELRPSPSDLRIT
                     RRLVELGELLGIPVLDHLIVTDEGMTSFVGVGLLPGPKGAPEEVE"
     gene            complement(2254..2946)
                     /locus_tag="VGB85_24470"
     CDS             complement(2254..2946)
                     /locus_tag="VGB85_24470"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEY0137269.1"
                     /translation="MTNHRPLPARRPGLLLALLAASLACGPAPAPAHDPAPGAPPPAA
                     PVTDAPVILALGDSLTAGYGLAQSQAWPTLVQARLKAAGFKHRVVNAGVSGDTSAGGL
                     ARLDWQLSQKPAVVVLELGGNDGLRGLPVAHTRDNLAKIIQRCQAAGATVLLAGMQIP
                     PNMGPDYTTAFRDMFPSLAREFGVPLIPFLLEGVAGDTSLNQRDGIHPTAEGQAIVAE
                     TVWKSLEPLLRK"
     gene            3001..3678
                     /locus_tag="VGB85_24475"
     CDS             3001..3678
                     /locus_tag="VGB85_24475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0137270.1"
                     /translation="MIEIARLTKTLRSGDRPVQILHGIDLHVPAGEFVAIMGPSGSGK
                     STLLGLIAGLDTPTSGSVKLAGQELVGLGEDALARLRGRTLGFVFQSFHLIPTLTAEE
                     NVAVPLELRGDSNARARAKALLATVGLADRGHHLPGQLSGGEQQRVALARAFVAEPPI
                     LLADEPTGNLDSATGERVLELLADLRARMGTTMVLVTHDPGLAARADRVVTLKDGRID
                     SDHRRAL"
     gene            3675..6206
                     /locus_tag="VGB85_24480"
     CDS             3675..6206
                     /locus_tag="VGB85_24480"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY0137271.1"
                     /translation="MKASFLLRMAVREARTGWQRLLFFLLSIAVGVGALVGIASFSAN
                     LEGAIRREARTLMAGDLEVVSSQVFDDETRAAIAAWDDEGARTVELAELPSMAASADG
                     TRTQLVEIKAVAGDYPFYGDLVLEPARPLAELTAGGKVVVEEALLLQLGAAVGDTVKL
                     GNRDFTIAGLVRREPDRIGGAFSLGPRVLMALPDVQSAGLITVGSRVRHRVLMALPPG
                     VDADTVQARLGEQLPHDRVSVRSFEEAQPTLRLFLARMGDFLRLATLVTLVLGGLGVA
                     QSIRVFLQQKQDTVAILKVVGATTREVTAVFLIQCMGLALIGSVMGLGLGVLIQAVLP
                     RVVGELLPVEIVGEFVPAAAAEGLAVGTITALLFCLVPLLSIRGVAPAQILRRELAAV
                     PPVADRRARALAVLVLGGGLVALAAWLSGSWRLGTIFVGGVAAALLVLAAASTATLVL
                     LRKLPRARSLAVRHGLGNLTRPGSQVAAVVVSLGLGVAVIAQIQLVQSGLLARVADDA
                     PADAPNFFFIGILPEQVDPFRALLAAQGALEGHRLVPIVQARLARVAGREVADMKFAS
                     ESEERTITREFAITWLNEMPPGNEVVAGRWWTADEAANQSLVSVSADLSQRLALEVGD
                     EVVFDIQGVRVPTQVQSKREVDWGQLQTNFVFILTAKALTGAPASWAATARSRPGVEG
                     RRALQAAVIAAMPNVTAIDAAEVIERVQGMVDRIAGVIRFMAGASILAGLVILGGSIA
                     ATRFRRVREAAILKSLGATRGVLLRSLAIEYVTLGVLAGVVGTAVGAALSWAVLTLVL
                     NIPWHAPVAVLVGLPLATAALTVLVGLGALLGVISEKPLAVLRGE"
     gene            complement(6227..6775)
                     /locus_tag="VGB85_24485"
     CDS             complement(6227..6775)
                     /locus_tag="VGB85_24485"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEY0137272.1"
                     /translation="MPAQSPDGVLEFWFSDAARPHWFVRSEAFDARVRDVLGPLHTQA
                     AAGQLDHWVRDPRGALALVILLDQVPRNIHRGSANAFATDPLALRHARDAVDLGLDRE
                     LGEDERVFLYLPFEHSESLADQDRCVTLMATLRNPMWLDYAERHRQVIARFGRFPHRN
                     ATLGRASSDEELAFLTQPGSSF"
     gene            complement(6818..7732)
                     /locus_tag="VGB85_24490"
     CDS             complement(6818..7732)
                     /locus_tag="VGB85_24490"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0137273.1"
                     /translation="MPSAHLGELAALTTAVCWAVSSLAFTRAGRRIGSVALNLIRLGM
                     GFGFSMILGLVLRGMPLPLDATAAQWGWLSLSGLVGFVFGDLCLFQAFVLLGPRLSLL
                     LMATAPPMTALLGWLVLGETLSLTAIAGMSLTLAGIAWVLLARPAEASPIGQEPRTST
                     LRRGIWLGLGGALGQAGGLVLSKHGMGDYDAFAATQVRILAGLAGFAALYTAIGFWPR
                     FWATCRDRVAMRQAAFGAVLGPFLGVSMSLIAVQRTHAGVAASIMATTPLLVIPLAAW
                     IDREKIRWPDLAGAVLAIAGVALLFQGP"
     gene            7849..8289
                     /locus_tag="VGB85_24495"
     CDS             7849..8289
                     /locus_tag="VGB85_24495"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HEY0137274.1"
                     /translation="MEALLELLTAGEASLWTMFVVAFLAATVLPVGSEPLFIALVRIH
                     PEQVTATIAVATLGNTLGGMTSYALGRFFSAKVAPATTQSRPARWLRRWGAPALLLAW
                     FPFGGDALCVLAGWMRMSWWQSMLWMALGKLARYWLLAEGTALF"
     gene            complement(8296..>9191)
                     /locus_tag="VGB85_24500"
     CDS             complement(8296..>9191)
                     /locus_tag="VGB85_24500"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0137275.1"
                     /translation="GEVLALIGPNGAGKSTTLRILATLQCPDKGAVRWDGRDAWPARY
                     QIRQRIGFLGDGTALYPGMTAAGYLQFFAECYGQSDRDARARVTELLQIFDLSSKADA
                     RVSEMSKGMRQRLSIARTLVHRPELLLLDEPADGLDPLARRQLREILRQVAGEGVGIV
                     ISSHILRELDGFCDAVAVIQKGKLEVAGPVEEVINRYEVGRRVHEVQITRGLQAALEI
                     LRKHEGLIESLAPLHKGEEIPDPATADRGLVRVRIHGSEERAAHLLRELVLADVEVIS
                     LSKVRSDLEDVYSSLGRDKVS"
BASE COUNT         1212 a         3022 c         3648 g         1309 t
ORIGIN      
        1 gagcagacgc tgcgctcgct ggcgctgcgg gcccaggagc gcgggctggc gctcgactgc
       61 acgatcgcgc cggaggtgcc ggcgtacgtc gtcggcgacc cggaccggct gcggcaggtg
      121 ctcgtcaacc tcgtcggcaa cgcgatcaag ttcaccgaga aggggcgcgt gacggtgcgc
      181 gtgcgggcgg ccccgggtcc gggcccgcgg gtggtcttcg agatcgaaga caccggcatc
      241 ggaattcctc ccgagaggtt gcgggcgatc ttcgtgccct tcgagcaggc cgacccgtcg
      301 acgacgcggc gcttcggcgg caccggcctg ggcctgacga tcgccagccg cctggtcggc
      361 ctgatgggcg gcgagatcgg cgtggcgagc gaggtcgggg tcggcagcac cttcaccttc
      421 gatgtcctgt tctcgccggt cagcagcgag acgccgacgc gccgggccgc gccgccgccg
      481 ctgtcgacgc gcgcagcagg caagcgtcgc ctcgagatcc tgctggccga ggacaacccg
      541 atcaatcaac gcgtggcggt cgagatcctc aagcgcatgg gccacaccgt cacggtcgtc
      601 aacgacgggc gcgcggccgt cgaggcggtc gagaacggcc gcttcgacct ggtactgatg
      661 gacgtgcaga tgccgatcat ggacggctgg caggcgacgg cgacgatccg ccgcagcccg
      721 cacgcgcacg cgcgacacac ccgcgtggtc gcgctgacgg cccgtgcgac cgacgaggac
      781 cgcaagcgct gcatggccca cggcatggac ggcttcttga ccaagccgct gcaggtcgcc
      841 gagctgctgc acctgtgcga ggagggcaag gcgcccgagc gcgccgcggc gcccgtgacc
      901 gcgagatcgc cgacgccgtc cacttcgtcg ccgtcatcca catcatctac atcatctaca
      961 ccgtccggcg agcgccagtc gatcgacgac tggcccgggg tgctgcagcg cgtcggcggg
     1021 tcgccggcga tgctggccag gctggtcggg atgtaccgcg acgagctgcc ggcggtgctc
     1081 gccgagctgc gcgcgacgct gatcagcggc gagggcctgc gccgcgccgc ccaccgcatc
     1141 gccgggacgg tcggcatctt caacgcccgc cacgcgctcg cgctcgcgcg ggaggtcgag
     1201 gagcgcgcgc cccagcgagc gcccgacctc ggcgcccgcg tcgaggcgct gtgcgccgag
     1261 ctgacccgcc tcgaccgcga gctgttcacg cgcagccagg cgttcggctc gggctgaccg
     1321 aaggggcatg tcctcggggg catgtcccgc cggtcggacg gggtggcccg cgggcgggac
     1381 aggcggtgcc gggcgggacg gcgaagtgcg cgacgggagg gacgcgcggg gctggcacgc
     1441 gaattgaaca gcgtcgcgta tgcacgagaa catctcctat caggccgagt atgcgcggcg
     1501 cgagcgggtg gcgggccctc cggtcggctt cgtgcccgag gacgacgaga tcgaggtcgc
     1561 gacgatcgtg ccctcggcga tcgagcggcc gcgcgagcgg atgttcgcgt tcgggccggc
     1621 ggccctgagc aacgtcgagc tggtggcgct gctgctcggc ggcggtcgct cggagcagcg
     1681 ggcgctggcg ctgctgcagc aggtcggcgg catcggcggg ctgctgcgct gtgtgccggg
     1741 cgagctgctc gagacgccag gggtcggcga ggcgtcggcg acggcggtgt gcgccgcggt
     1801 cgagctggcg cggcggatcg ggcagatcga gatgcccttc gagtcgccga tccagggccc
     1861 ggaggacgtg cgcaggtacg tccgggcgag cctgcgcgga cgggcccagg agatctttat
     1921 ggtgctcggg ctcgacgggc gccagcgcgt gcgcctggtc cgcgaggtgg cgatcggctc
     1981 actttcccac gtcgaggtcc acccgcgcga ggtgtttcgc ccgctggtcc gcgccggctc
     2041 acactcggtg atcctcgtgc ataaccaccc cagcggcgag ctgcgcccga gcccgtcgga
     2101 cctgaggatc acgcgcaggc tcgtcgagct cggggagctc ctcgggatcc cggtgctcga
     2161 ccacctgatc gtcaccgacg agggcatgac ctcgttcgtc ggcgtcggcc tgctaccagg
     2221 accgaagggc gcgccggagg aggtcgagtg aggtcacttg cgcagcagcg gctccagcga
     2281 cttccacacg gtctcggcga cgatcgcctg accctcggcg gtcgggtgga tgccgtcgcg
     2341 ctggttgagg ctggtgtcac cggcgacgcc ctcgagcaag aaggggatca gcggcacgcc
     2401 gaactccctg gcgagcgagg ggaacatgtc gcgaaaggcg gtcgtgtagt cggggcccat
     2461 gttgggcggg atctgcatgc cggcgagcag caccgtcgcg ccggcggcct ggcagcgctg
     2521 gatgatcttc gccaggttgt cgcgcgtgtg cgcgaccggc agcccgcgca ggccgtcgtt
     2581 gccgccgagc tcgagcacga cgacggctgg cttttgggac agctgccagt cgagccgcgc
     2641 gagcccgccg gcgctggtgt ccccgctgac gcccgcgttg acgacccgat gcttaaaacc
     2701 ggcggccttg agccgggcct ggaccagcgt cggccacgcc tgcgactgtg cgaggccgta
     2761 gccggcggtc aggctgtcgc cgagcgcgag gatcaccggg gcgtcggtga cgggcgcggc
     2821 gggaggcggg gcgcccgggg ccgggtcgtg tgccggcgcg ggggcaggac cgcaggccag
     2881 cgaggcggcg aggagggcga gaaggaggcc ggggcggcgc gcgggcaggg ggcggtggtt
     2941 cgtcatctcg tcaaccttcg tgctccgggg ggagttgcag agtaccttgt cgcggcgccc
     3001 atgatcgaga tcgcacggtt gaccaagacg ctgcgctccg gggaccgccc ggtccagatc
     3061 ctccacggca tcgacctgca cgtgccggcc ggcgagttcg tggcgatcat ggggccctcg
     3121 ggctcgggca agtcgacgct gctcgggctg atcgccgggc tcgacacgcc gaccagcggc
     3181 agcgtcaagc tggcggggca ggagctggtc gggctcgggg aggacgcgct ggcccgcctg
     3241 cgcggtcgca cgctcggctt cgtgttccag tcgttccacc tgatcccgac gctgacggcg
     3301 gaggagaacg tcgcggtgcc gctcgagctg cgcggggaca gcaacgcgcg cgcgcgagcg
     3361 aaggcgctgc tggcgacggt cgggctggcg gatcgcgggc atcacttgcc gggtcagctc
     3421 tcgggcggag agcagcagcg ggtcgcgctg gcgcgggcct tcgtcgccga gccgccgatc
     3481 ctgctggcgg acgagccgac cggcaacctc gactcggcga ccggcgagcg cgtgctcgag
     3541 ctgctcgcgg acctccgggc gcgcatgggc acgacgatgg tgctggtgac gcacgacccg
     3601 gggctggcgg cgcgggccga ccgcgtggtc acgctcaagg acggccgcat cgactccgac
     3661 catcggcggg ccctgtgaag gcgagctttt tgctgcgcat ggccgtccgc gaggcccgta
     3721 cgggctggca gcggctgttg ttctttttgc tgtcgatcgc ggtcggcgtg ggggccctcg
     3781 tcgggatcgc gtcgttctcg gcgaacctcg agggggcgat ccgccgcgag gcccggacgc
     3841 tgatggcggg cgacctcgag gtggtgtcgt cgcaggtctt cgatgacgag acacgcgccg
     3901 cgatcgcggc gtgggacgac gagggcgcgc gcacggtcga gctcgcggag ctgccgtcga
     3961 tggccgccag cgccgacggc acgcgcacgc agctggtgga gatcaaggcg gtcgcgggtg
     4021 attatccgtt ttatggcgac ctcgtgctcg agccggcgcg tccgctggcg gagctgaccg
     4081 cgggcggcaa ggtggtcgtc gaggaggcgc tgctgctgca gctcggcgcg gcggtcggtg
     4141 acacggtcaa gctcggcaac cgcgacttca cgatcgccgg gctcgtgcgc cgcgagccgg
     4201 accgcatcgg cggggcattt agcctcgggc cgcgcgtgtt gatggcgctg ccggacgtgc
     4261 agtccgcggg cctgatcacg gtcggcagcc gcgtgcgcca ccgcgtgctg atggcgctgc
     4321 cgcccggggt cgacgcggac acggtgcagg cgcggctcgg cgagcagctg ccgcacgatc
     4381 gcgtcagcgt gcgcagcttc gaggaggccc agccgacgct gcgcctgttc ctggcgcgca
     4441 tgggcgactt cttgcggctg gcgacgctgg tgacgctggt gctcggcggt ctcggcgtgg
     4501 cccagagcat ccgcgtgttc ctgcaacaaa agcaggacac ggtggcgatc ctcaaggtgg
     4561 tcggcgcgac gacccgcgag gtgacggcgg tgttcctgat ccagtgcatg gggctggcgc
     4621 tgatcggcag cgtgatgggg ctcgggcttg gggtgctgat ccaggccgtg ctgccgcgcg
     4681 tggtcggtga gttgctgccg gtcgagatcg tcggcgagtt cgtgccggcg gccgcggccg
     4741 aggggctcgc ggtcggcacg atcaccgcgc tgctgttctg cctggtgccg ctgctgtcga
     4801 tccgcggggt cgcgccggcg cagatcctcc ggcgcgagct ggccgcggtg ccgccggtcg
     4861 cggatcgcag ggcgcgcgcc ctggcggtgc tggtgctcgg gggcgggctc gtggcgctcg
     4921 cggcctggct gtcgggctcg tggcggctcg ggacgatctt cgtcggcggg gtcgcggccg
     4981 cgctgctggt gctcgccgcg gcgagcacgg cgacgctggt actgctgcgc aagttgccgc
     5041 gcgcgcgctc gctggcggtg cggcacgggc tcgggaacct gacacgaccg ggcagccagg
     5101 tcgcggcagt ggtggtgtcg ctcggcctcg gcgtcgcggt gatcgcccag atccagctgg
     5161 tccagtcggg cctgctcgcg cgcgtcgccg acgacgcacc ggccgacgcg ccgaacttct
     5221 tcttcatcgg cattctcccg gagcaggtcg atccgttccg ggcgttgctg gcagcgcagg
     5281 gggccctcga gggccatcgc ctggtcccga tcgtgcaggc ccgactcgcg cgggtcgccg
     5341 ggcgcgaggt cgcggacatg aagttcgcca gcgagtcgga ggagcgcacg atcacgcgcg
     5401 agttcgcgat cacgtggctc aatgagatgc cgccgggcaa cgaggtggtg gccggtcgct
     5461 ggtggacggc cgacgaggcc gcgaaccagt cgctggtgtc ggtctcggcc gacctgtcgc
     5521 aacgactggc gctcgaggtc ggcgacgagg tggtgttcga tatccagggc gtgcgtgtgc
     5581 cgacgcaggt gcagagcaag cgcgaggtcg actggggcca gctgcagacc aacttcgtgt
     5641 tcatcctgac ggcgaaggcc ctgaccggcg cgccggcctc gtgggccgcg acggcccgct
     5701 cgcgtcccgg cgtcgagggg cgccgggcgc tgcaggcggc ggtgatcgcc gcaatgccga
     5761 acgtgacagc gatcgacgcg gccgaggtga tcgagcgcgt gcagggcatg gtcgaccgca
     5821 tcgccggggt cattcgcttc atggccggcg cgagcatcct ggcggggctc gtcatcctcg
     5881 gcggatcgat cgcggcgacc cggtttcgcc gcgtgcgcga ggcggctatt ttgaagtcgc
     5941 tcggggcgac gcggggggtg ctgctgcgct cgctggcgat cgagtacgtg acgctcgggg
     6001 tgctcgcagg cgtggtcggc acggccgtcg gcgctgccct gtcgtgggcc gtgctgacgc
     6061 tggtgctcaa catcccctgg cacgcgccgg tggcggtgct cgtcgggctg ccgctggcga
     6121 ccgcggcgct gacggtgctc gtcgggctcg gcgcgctgct cggggtgatc tccgagaagc
     6181 cgctcgcggt gctgcgcggg gagtgagacc tgtctgcgag gacaggtcag aacgaggagc
     6241 cgggctgggt caggaacgcg agctcctcgt cgctgctggc gcggccgagg gtcgcgttgc
     6301 ggtgcgggaa gcggccgaag cgggcgatga cctggcggtg gcgctcggcg tagtcgagcc
     6361 acatcgggtt gcgcagcgtc gccatgaggg tgacgcagcg gtcctggtcg gcgagcgact
     6421 cgctgtgctc gaaggggagg tagaggaaca cgcgctcgtc ctcgccgagc tcgcggtcga
     6481 ggccgaggtc gacggcgtcg cgggcgtgac gcagcgcgag ggggtcggtg gcgaaggcgt
     6541 tcgcgctgcc gcggtggata ttgcgcggga cctggtcgag caggatgacc agggcgaggg
     6601 cgccgcgggg gtcgcggacc cagtggtcga gctggcccgc ggcggcttgc gtgtgcaagg
     6661 ggccgagcac gtcgcggacg cgggcgtcga aggcctcgct gcgcacgaac cagtgcgggc
     6721 gcgcggcgtc cgagaaccag aactcgagca caccatcggg gctttgagcc ggcatgacct
     6781 cgaggatccg gcagcggcct tgcgcttgca aggccgctca cgggccctgg aacagcaggg
     6841 cgacgcctgc gatcgccagc acggcgccgg cgaggtcggg ccagcggatc ttctcgcggt
     6901 cgatccacgc ggcgagcggg atgacgagca gcggcgtggt cgccatgatg ctggcggcga
     6961 caccggcgtg ggtgcgctgc acggcgatca gcgacatcga gacgccgagg aaggggccga
     7021 gcaccgcgcc gaaggcggcc tgacgcatgg cgacgcggtc gcggcaggtg gcccaaaacc
     7081 gcggccagaa gccgatcgcg gtgtagagcg cggcgaagcc ggcgagaccg gcgaggatcc
     7141 gtacctgggt ggcggcgaag gcgtcgtaat cgcccatgcc gtgcttgctg agcacgagac
     7201 cgccggcctg gccgagcgcg ccgccgaggc cgagccagat gccgcgacgc agggtcgatg
     7261 tccgcggctc ctgaccgatc gggctcgcct cggcgggccg cgcgagcaac acccaggcga
     7321 tgccggcgag ggtcagcgac atgccggcga tcgcggtcag cgacagggtc tcgccgagca
     7381 ccagccagcc gagcagcgcg gtcatcggcg gggcggtcgc catcaacagc aacgacaggc
     7441 gcgggccgag cagcacgaag gcctggaaca gacacaggtc gccgaacacg aagccgacga
     7501 ggcccgagag cgagagccag ccccactgcg cagcggtggc gtcgagcggc agcggcatgc
     7561 cgcgcagcac gaggccgagg atcatggaga agccgaaccc catgccgagg cggatcaggt
     7621 tgagcgcgac cgagccgatc cgccggcccg ccctcgtgaa ggcgagcgag gacacggccc
     7681 agcacacggc ggtggtgagc gcggcgagct caccgaggtg ggcgctgggc acggcgggac
     7741 tgtagcggag cgcgccagcg gcggcccgtg gcgcgggcgc gagtcaggtc tcatcggccc
     7801 ggccgtgagg cgcccgggcc ggcgtcgggc cggctagact ggcgcggcgt ggaggcgttg
     7861 ctcgagctgt tgaccgcggg cgaggcgtcg ctgtggacga tgttcgtggt cgcgttcctg
     7921 gcggcgacgg tgctgccggt cggctccgag ccgctgttca tcgcgctggt gcggatccat
     7981 cccgagcagg tcacggcgac gatcgcggtg gcgacgctcg gcaatacgct cggcggcatg
     8041 acgagctacg cgctcgggcg cttcttcagc gccaaggtgg cgccagcgac gacgcagtcg
     8101 cggccggcac ggtggctgcg ccgctggggc gcgccggcgc tgttgctggc gtggttcccg
     8161 ttcggcggcg atgcattgtg tgtgctcgcg gggtggatgc gaatgtcgtg gtggcagtcg
     8221 atgctgtgga tggcgctcgg caagctggcc cgctactggc tgctcgccga gggcacggcg
     8281 ctgttttaaa aaaggtcagc tgaccttgtc gcggccgagg ctgctgtaca cgtcctcgag
     8341 gtcggagcgg acctttgaaa gcgagatgac ctcgacgtcg gcgagcacga gctcgcggag
     8401 caggtgagcg gcccgctcct cgctgccgtg gatccgcacg cgcacgaggc cgcggtcggc
     8461 ggtcgcgggg tcggggatct cctcgccctt gtgcagcggc gcgaggctct cgatcaggcc
     8521 ctcgtgcttg cgcaggatct cgagcgccgc ctgcagacca cgcgtgatct ggacctcgtg
     8581 tacgcggcgg ccgacctcgt agcgattgat gacctcctcg accggaccgg cgacctcgag
     8641 cttgcccttt tggatcacgg cgacggcgtc gcaaaagccg tcgagctcgc gcaggatgtg
     8701 actgctgatg acgatgccga cgccctcgcc ggcgacctgc cgcaagatct cccgcagctg
     8761 gcggcgcgcg agcgggtcga ggccgtcggc gggctcgtcg agcaacagca gctcggggcg
     8821 gtggaccagc gtgcgggcga tcgacaggcg ctggcgcatg cccttgctca tctcggagac
     8881 gcgcgcgtcg gccttgctgc tgaggtcgaa gatctgcagc agctcggtga cgcgggccct
     8941 ggcgtcgcgg tcgctctggc cgtaacactc ggcgaagaac tgcaggtagc cggcggcggt
     9001 catgccgggg tacagggcgg tgccgtcgcc gaggaagccg atccgctggc ggatctgata
     9061 gcgcgcgggc caggcgtcgc ggccgtccca gcgcacggcg cccttgtcgg ggcactgcaa
     9121 ggtggcgagg atccgcagcg tggtgctctt tccggcgccg ttggggccga tcagcgcgag
     9181 cacctcgccg c
//
LOCUS       DASWNJ010001472         2691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_591829, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001472 DASWNJ010000000
VERSION     DASWNJ010001472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591829"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2691)
                     /locus_tag="VGB85_24505"
     CDS             complement(<1..>2691)
                     /locus_tag="VGB85_24505"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0137276.1"
                     /translation="SSINGADGSEGGDWVPPQSFDEYRLIRLLGRGSMGSVYLAHDDV
                     LDRPVAVKFIGNIAPDAEDRERFLVEARAVARIQHPNVMVIYRVGELEGHPFLITEYI
                     RGKSLADLTVPLPWRKVLELGIGLARGLATAHRMGVLHRDIKLANAVLSEDGEVKLLD
                     FSLAKLLDESVPELVSEHDQQDIAGSKRAAVSAVERHLAEGRPGRPGRVDRMAETIKV
                     AEALEAAVAAAAAASAGAASDVPADASGGWMRRSSLTQAGTLLGTPHYMAPELWRAEA
                     ASRCSDVYALGVLLYILCAGRPPTEATSPVELATRVQEQEPRPLLERAPRADPRLAAI
                     IDRCLRRDPFARFSSADDLRSALEQLTPLGRELAVPEGNPYRGLQAFEAQHRALFFGR
                     AAEIRAVVERLRGEALVVVAGDSGSGKSSLCRAGVLPLVEEGSLEPGRSWASAVMTPG
                     RYPLQTLVATLAGLFDMTEETVSALIAGEPEALIRALRKQLGDVRGRLIFVDQLEELV
                     TLGEAGQVTVVGHVLARLASGIPGLRLITTVRGDFLTRVAQIPALGDEIGRAIYLLRP
                     LSPEGAREAIVGPAQTKAVSFENEALVDDLVKAGSRGSLPLLQFALAELWEIRDKASA
                     LITAADLTKIGGVTGALARHADGTLAQLLPAQRRAGRRVLMRLVTLEDTRASLTEDEL
                     TAGEAPAKAALGALVNARLVVAREVGERVVFEIAHEALLAGWSTLRAWLDEERESRAV
                     RHRLELAVADWDRLGRTSDGLWTTVQLKETALLEPETLRPLEREFLAASRAAMARGRR
                     LRRGAIVLLPVIALLTYGGIWFKGQADLGRRIDAHVAVASDEIASAEQLQAAATLLRE
                     QALGLFDDAKATAGESLWLQYRDNIAQIDDSLGKAAQELEM"
BASE COUNT          349 a         1012 c          982 g          348 t
ORIGIN      
        1 catctcgagc tcctgcgcgg ccttgccgag gctgtcgtcg atctgcgcga tgttgtcgcg
       61 gtactgcaac cacagcgact cgccggcggt cgccttggcg tcgtcgaaca ggccgagcgc
      121 ctgctcgcgc agcagcgtgg ccgccgcctg cagctgctcc gcggaggcga tctcgtcgct
      181 cgcgacggcg acgtgggcgt cgatcctgcg cccgaggtcg gcctgtccct tgaaccagat
      241 cccgccgtag gtcagcagcg cgatcaccgg cagcagcacg atcgccccgc gacgcaggcg
      301 acgcccgcgg gccatcgccg cgcggctggc ggccaggaac tcgcgctcga gcgggcgcag
      361 ggtctcgggc tcgagcagcg ccgtctcctt gagctgcacc gtcgtccaca gcccgtcgct
      421 cgtgcggccg aggcggtccc agtcggcgac cgccagctcg aggcgatggc ggaccgcccg
      481 gctctcgcgc tcctcgtcga gccacgcgcg cagcgtgctc cagccggcca gcagcgcctc
      541 gtgggcgatc tcgaagacca cgcgctcgcc gacctcgcgg gcgacgacga ggcgagcgtt
      601 gaccaacgcg ccgagcgccg ccttcgcggg cgcctcgccg gcggtcagct cgtcctccgt
      661 caggctcgcg cgcgtgtcct cgagcgtgac gaggcgcatc agcacgcggc gaccggcgcg
      721 gcgctgggcc ggcagcagct gcgcgagcgt gccgtcggcg tgccgcgcga gcgccccggt
      781 gacgccgccg atcttggtca ggtcggccgc ggtgatcagc gccgacgcct tgtcgcggat
      841 ctcccacagc tcggcgaggg cgaactgcaa cagcggcagg ctgccgcgcg agccggcctt
      901 gaccaggtcg tcgacgagcg cctcgttctc gaagctgacc gccttggtct gcgccggccc
      961 gacgatcgcc tcgcgcgcgc cctcgggtga cagcggccgc aggaggtaga tcgcacggcc
     1021 gatctcgtcg ccgagcgcgg ggatctgggc gacgcgggtc aggaagtcgc cgcgcaccgt
     1081 cgtgatcagg cgcaggcccg ggatgcccga ggccagccgc gcgagcacgt ggccgacgac
     1141 cgtgacctgc ccggcctcgc cgagggtcac cagctcctcg agctggtcga cgaagatcag
     1201 ccgaccgcgc acgtccccga gctgcttgcg cagcgcccgg atcagcgcct ccggctcgcc
     1261 ggcgatcagc gcgctgactg tctcttcggt catgtcgaag aggccagcca gcgtggcgac
     1321 gagggtctgt aatgggtacc ggcccggcgt catcacggcg ctggcccacg agcggcccgg
     1381 ctcgaggctg ccctcttcca cgagcggcaa cacgccggcg cggcacagcg acgacttgcc
     1441 cgagcccgag tcgccggcga cgacgacgag cgcctcgcca cgcaggcgct cgacgaccgc
     1501 ccggatctcg gcggcgcggc cgaagaacag cgcccggtgc tgggcctcga acgcctgcaa
     1561 gccgcggtac gggttgccct ccggcaccgc cagctcgcgg cccagcggcg tcagctgctc
     1621 gagcgcggag cgcaggtcgt cggccgagct gaagcgcgcg aaggggtcgc ggcgcagaca
     1681 ccggtcgatg atcgcggcga gccgcgggtc ggcccgcggc gcccgctcga gcagcggccg
     1741 cggctcctgc tcctgcaccc gcgtcgccag ctcgaccggc gaggtcgcct cggtcggcgg
     1801 ccggccggcg cacaggatgt acagcagcac cccgagcgcg tagacgtccg agcagcgcga
     1861 ggccgcctcg gcgcgccaca gctccggcgc catgtagtgc ggcgtaccga gcagcgtgcc
     1921 ggcctgcgtc agcgacgagc ggcgcatcca cccgcccgag gcgtcggccg ggacgtcgct
     1981 cgccgctccg gccgacgccg cggccgcggc cgccaccgcg gcctcgagcg cctcggcgac
     2041 cttgatcgtc tcggccatgc ggtcgacgcg gccggggcgg ccggggcggc cctcggcgag
     2101 gtggcgctcg accgccgaga ccgccgcgcg cttcgagccg gcgatgtctt gctggtcgtg
     2161 ctcgctgacc agctcgggca ccgactcgtc gagcagcttg gcgaggctga agtcgaggag
     2221 cttgacctcg ccgtcctcgc tgagcaccgc gttggcgagc ttgatgtcgc ggtgcagcac
     2281 gcccatgcgg tgcgccgtcg ccaggccgcg cgcgaggccg atgccgagct ctagcacctt
     2341 gcgccacggc agcggcaccg tcaggtccgc gagcgacttg ccccggatgt actcggtgat
     2401 caggaagggg tggccctcga gctcgccgac gcgatagatg accatcacgt tcgggtgctg
     2461 gatgcgggcg accgcgcggg cctcgacgag gaagcgctcg cggtcctcgg cgtccggggc
     2521 gatgttgccg atgaacttga ccgcgaccgg acggtcgagc acatcgtcgt gcgcgaggta
     2581 gacgctgccc atgctgccgc gcccgagcag gcgaatgagc cggtactcgt cgaagctctg
     2641 cggcggcacc cagtcgccgc cctcgctgcc gtccgcgccg ttgatcgagc t
//
LOCUS       DASWNJ010001473         2838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_130758, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001473 DASWNJ010000000
VERSION     DASWNJ010001473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130758"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..412)
                     /locus_tag="VGB85_24510"
     CDS             complement(173..412)
                     /locus_tag="VGB85_24510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137277.1"
                     /translation="MKPSRKSIRLPDLSLAGAERRLCLEALTRTGSLVEAAELLGTNR
                     HALRRLILKHEIVWWPYAQQQTQWKIPPRKRAPPR"
     gene            complement(409..861)
                     /locus_tag="VGB85_24515"
     CDS             complement(409..861)
                     /locus_tag="VGB85_24515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137278.1"
                     /translation="MAPKTDVTAPLPFFYHAASERVLYVAHGPSPSPLIFSIALRDVL
                     RHEPICRGPVQTNETHPFGTSTGVSSLVEEPVRRGLGEAEALDELGVLEDILLLWRDF
                     CDAVAERLQHGEDLEPALAKAFVLENAHDIDMSDLHANSAGEPHGGAA"
     gene            1213..1518
                     /locus_tag="VGB85_24520"
     CDS             1213..1518
                     /locus_tag="VGB85_24520"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY0137279.1"
                     /translation="MVRQLPKPRRTFVRTDEVLKAAGVSFVTLREWIRRGILPEPLET
                     ARGRGNLGKYPRSAVARAELARRLRDDGWTLDEIAEHMRSLSWETGSSTGGAVEPEA"
     gene            1522..>2838
                     /locus_tag="VGB85_24525"
     CDS             1522..>2838
                     /locus_tag="VGB85_24525"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0137280.1"
                     /translation="MIPDVLDGRYQLLEVLGLGGQGKVFRGRDLHTGANVAVKVMDDA
                     RASPEAVARFAQEGRLAARIRDPHLVAAMHFGVWQGYRYIVFDNLAGVAPVTTLLDRG
                     RMDPYVVCDLARQLLGALDALHQAGVAHLDLAPANCLWRERSSGRIEVFLADLGSAAA
                     AVPVTGGPRRSSEPVGTVHFMAPEMLEEAPVDQRADLWSLGALMYVLLTGIFVDLGDE
                     DEPLAVPPPAVLVPSIPAAISDIVMGALTTVERRFASAVAMMASIDAGLAGRPALLVA
                     SSRSPARVPIWAGVGGVALAAVLAVLGTYRILDAQSCESPSSPRPEIECVPATIEEPT
                     SVNARDTVSSSVTLADSEPPATRPPDGLTAGSSVPAAAVEAEPARTRPLQWSTVKRVV
                     QRQAPKLRKCSSDDAVTLGVRVKGGRVTLESFDGRPAAPLPHHECAR"
BASE COUNT          452 a          948 c          967 g          471 t
ORIGIN      
        1 ttgatcgccc cggcgaggtc gccatgattt tccagcgcgg tgatcttgtc gagcgtgtcg
       61 cgctgcgcct gcgaccaacc ggcgtgggcg aggcctggag gcgtggcgag cgtgacagcc
      121 agcccgagcg agagaccgag gcgcatatcc gcaccataac ataatcgccg gatcatcgtg
      181 gcggtgcccg cttgcgaggt gggatcttcc actgcgtctg ttgctgggcg taaggccacc
      241 acacgatctc atgcttcaag atcaatcggc gcagggcgtg gcgattggtg cccaacaact
      301 cggcggcctc gaccagcgat ccggtgcgcg tcagggcctc caggcacagc ctacgctcgg
      361 cgccggccag gctcaggtcg ggcagcctga tcgacttgcg actgggcttc atgccgcgcc
      421 cccgtgtggc tcgccggccg agttcgcgtg caggtcgctc atgtcgatgt catgagcatt
      481 ctcaagaaca aacgccttcg ccagcgctgg ctcgagatcc tcaccatgtt gcaggcgctc
      541 ggcgaccgcg tcgcagaagt cccgccatag cagcaagatg tcctcgagca cacccagttc
      601 atcgagagcc tcggcctcgc ccaacccgcg gcggacaggc tcctcgacga gtgacgagac
      661 tccggtcgac gttccgaagg gatgggtctc gttcgtttga accgggccgc ggcagatcgg
      721 ctcgtgtcgc agcacgtccc tgagcgcgat cgagaagatg agcggcgacg gtgacgggcc
      781 gtgagcgacg taaaggaccc gttcgcttgc ggcgtggtag aaaaatggca gcggcgcagt
      841 gacgtcggtc ttcggtgcca tcgagagcct gctttcacga ggcgtcacga atgcgcggcg
      901 gttgtaccga gcggcgcgct tgctcgacga agctcacgac gggcggcatc acgacggccc
      961 cgacgatcgg gcccgggagt cgagcggcga tgcagaccgc tgccatggcc acgcgcggat
     1021 cggccagcga cagagccctc ctggccgatg gccacgaagc ctgctgtttt ccgatcaaac
     1081 gcggcaacaa acaggctggg cggcgctttc atggttgttc ttctcgccat aaaagcttaa
     1141 gaaggactta gggaagatgc aagttaaatt acagagtgca gccccgggta gtttcagcct
     1201 aaactcgcgc ccatggtgcg ccagctaccc aaaccgcgcc gcacgttcgt gcgcaccgac
     1261 gaggtgctca aggccgcggg cgttagcttc gttactctgc gcgagtggat ccggcgcggg
     1321 atcctgcccg agccgctgga gactgcccgc ggccgcggca atcttgggaa atacccgcgc
     1381 tcggccgtcg cgcgagccga gctcgctcga cggctccgcg atgacggctg gacgctggac
     1441 gagatcgccg aacacatgcg cagcctgtcc tgggagaccg ggtcctcgac cggtggggcg
     1501 gtcgagccgg aggcctgagc gatgatcccc gacgtcctcg acggccgcta ccagttgctc
     1561 gaggtgcttg gcctgggcgg acagggcaag gtgttccgcg gacgggacct gcataccggc
     1621 gccaacgtgg cggtcaaggt catggacgac gcccgggcca gccctgaagc cgtggcgagg
     1681 ttcgcccagg agggccgcct tgccgcccgg atccgcgatc cgcacctggt cgcggccatg
     1741 cactttggcg tgtggcaggg gtaccgctat atcgtcttcg acaacctcgc cggtgtcgct
     1801 ccggtgacga cgctgctgga caggggccgg atggacccct acgtggtttg tgacctggcg
     1861 agacagctcc tcggggccct cgacgcgctc caccaggcgg gcgtcgcaca cctcgatctg
     1921 gcgccggcga actgcctctg gcgcgagcgt tcgtcaggtc ggatcgaggt ctttctggcg
     1981 gacctggggt cggcggcggc ggcggtcccc gtgaccggcg ggccgcggag gtccagcgag
     2041 ccggtcggca ccgtgcactt catggccccg gagatgctcg aagaggcgcc cgtcgatcag
     2101 cgcgcggacc tctggtctct cggtgctttg atgtacgtgc tcttgaccgg catctttgtc
     2161 gacctgggcg acgaggatga accgctcgcg gttcccccgc ctgcagtcct cgtcccttcg
     2221 atcccggcgg cgatcagcga catcgtgatg ggcgcgctca ccactgtgga gcggcgattc
     2281 gcgtccgcgg tggcgatgat ggcgagcatc gacgccggcc tcgccggccg cccggcgctg
     2341 ctcgtcgcgt cttcacgctc cccggcaagg gtgccgatct gggccggcgt agggggcgtg
     2401 gcactcgcgg ctgttctcgc tgtgctgggc acgtatcgca tactcgacgc tcagtcctgt
     2461 gaatcgccca gctcgcccag gcccgagatc gagtgcgtcc ccgccaccat cgaagagccg
     2521 acatccgtga acgcgcgaga tacggtgtcg agctccgtca ccctcgctga cagcgagccg
     2581 cctgcgacgc gcccacccga cggcctcacc gccggctcgt ctgtcccggc cgcagcggtg
     2641 gaggccgaac cagcgcgcac gaggcccctc caatggtcga cggtgaagcg cgtcgtccag
     2701 cggcaggctc cgaagctccg caagtgctct tcggatgacg cggtgacact cggcgtccgg
     2761 gtgaagggcg gacgagtcac ccttgagagc ttcgatggaa gacccgcggc gccgcttcca
     2821 caccatgagt gcgcacgc
//
LOCUS       DASWNJ010001474         2926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_96673, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001474 DASWNJ010000000
VERSION     DASWNJ010001474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96673"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..1556
                     /locus_tag="VGB85_24530"
     CDS             27..1556
                     /locus_tag="VGB85_24530"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY0137281.1"
                     /translation="MTKHDGVGRWSRRGLLGAGMAAGGLLAGDLFAGDFAMQRLVQAG
                     SAIGRRFVFIYVPGGWDQLLFLDPREFEYGLDQDAYQKEVERTGIDTSYARGGYNNDL
                     RDEFGEGLNRPAGADPAFSFGPAAVRRDKNKQPIDADNLVALAEQGVPMAIIRGINMG
                     TLGHEPGYVYFLTGEPAVGSSARGASMPVRLASQIGKLGGDDQTVVSNLAVEVDSYTG
                     NADGRYAALRLGTITDSERLLMRETKLGEAPGVEAALATHAGRKRSPAAELLDGDGLL
                     SQYAAASGRARELIESDVIQHFEFMSGNDPESQALRAAYHVQGDDPRQPAAKAAFTAQ
                     AIKYGLAQFISVSFPGGGDSHGGGNRGHATGLHEAVRALADLVRDLAKTPAEAPLAGT
                     WLDNTTIVMFSEFGRTPRFNSIGGRDHHFSNSCLLVGAGIQAGRVVGGTTEVGGMQPK
                     VYDFTGQKSLGDDAIPTGTDQRHITPADIGATLLASVGGEYWEYRDAVPLWQAITQTP
                     F"
     gene            1735..1968
                     /locus_tag="VGB85_24535"
     CDS             1735..1968
                     /locus_tag="VGB85_24535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137282.1"
                     /translation="MEQQNLVKLRAVKHGDSGIALEFTGVTGDIGKYFAAQPDGGELP
                     LEYVEAILRFYLTNSSGRQGVDPGGNAPVHALW"
     gene            1985..>2926
                     /locus_tag="VGB85_24540"
     CDS             1985..>2926
                     /locus_tag="VGB85_24540"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0137283.1"
                     /translation="MAALMPKRSVEKPTTVDCAIIGGGVAGLYAGWRLATGASVAQST
                     DAPGSLAVFEHSERLGGRLDTGTVGGTPVEFGAMRYLPAQKLLDALLHAGLPGLSTTP
                     FVSTQLQILYLRGVFMRPLASTPSPYQLVGAEKDKSALELLAGAIEALVPGALELTPA
                     QWEVATTRPVYDGYALYQLGLWNVLSMVLSNEAYKYVFDALGIESVLSNWNAAEGLQL
                     LTFILRALAGGPPGAFHRLVDGWSRLTDTLAAQLSAHGGEIHRQHTLVALLPQHGHEL
                     SARDEEYVDVAAIKAAITDSIASQGGDLHASHGMVTLV"
BASE COUNT          456 a         1022 c         1017 g          431 t
ORIGIN      
        1 acgtgtactg aggacaggag agcatgatga ccaagcacga cggtgtgggc agatggagcc
       61 gccgggggct gctcggtgcg ggcatggcgg ccggcgggtt gctggccggt gacctgttcg
      121 cgggtgactt cgcgatgcag cgactcgtgc aggccggcag cgcgatcggc cgccgcttcg
      181 tcttcatcta cgtgcccggc ggctgggatc agctgctgtt cctcgacccg cgcgagttcg
      241 agtacgggct cgaccaggac gcctaccaga aggaggtcga gcgcacgggg atcgacacgt
      301 cgtacgcgcg cggcggctac aacaacgacc tgcgcgacga gttcggcgag ggcctcaatc
      361 gtccggccgg cgccgacccg gccttctcgt tcggaccggc cgcggtccgc cgcgacaaga
      421 acaagcagcc gatcgacgcc gacaacctgg tcgccctggc cgagcagggc gtgccgatgg
      481 cgatcattcg cgggatcaac atgggcacgc tcgggcacga gccgggctac gtgtatttct
      541 tgaccggcga gccggcggtc gggtcctcgg cgcgcggggc gagcatgccg gtgcggctcg
      601 cgtcgcagat cggcaagctc ggcggcgacg accagacggt cgtctccaac ctcgccgtcg
      661 aggtcgacag ttacaccggc aacgccgacg gccgctacgc cgcgctgcgc ctcggtacga
      721 tcaccgactc cgagcggctc ctgatgcgcg agacgaagct cggcgaggcg cccggcgtcg
      781 aggcggcgct cgcgacccac gccggacgca agcgcagccc cgccgccgag ctgctcgacg
      841 gcgacggcct gctctcgcaa tacgccgccg cgagcggccg cgcgcgcgag ctgatcgagt
      901 ccgacgtcat ccagcacttc gagttcatgt ccggcaacga ccccgagagt caggccctgc
      961 gcgcggccta tcacgtccag ggcgacgatc cgcgccagcc agcggccaag gccgcgttca
     1021 cggcccaggc gatcaagtat gggctcgcgc agttcatctc ggtgtccttc cccggcggcg
     1081 gcgattcgca cggcggcggg aaccgtggcc acgcgacggg cctccacgag gccgtgcggg
     1141 cgctcgccga cctcgtccgc gacctcgcca agacgccggc cgaggcgccc ctcgcgggca
     1201 cctggctcga caatacgacg atcgtgatgt tcagcgagtt cggccgcacg ccgcgcttca
     1261 actcgatcgg cgggcgcgac caccacttct cgaattcatg cctgctcgtc ggcgcgggca
     1321 tccaggccgg acgcgtcgtc ggcggcacca ccgaggtcgg cggcatgcag ccgaaggtct
     1381 acgatttcac cggccagaag tccctgggcg acgacgcaat ccccaccggc accgatcaac
     1441 gccacatcac gcccgccgac atcggcgcga cgctgctcgc cagcgtcggc ggcgaatact
     1501 gggaataccg cgacgccgtg ccgctgtggc aggcgatcac gcagacgccg ttctagcgcg
     1561 cggcgacgcc cgtacgagcg aacaggcaga gcgagcaggc agggtccgcg ctgtcgccgt
     1621 ggcccgccgg ctcgcgggcg tatctgcgcg cgggcgcttc tgaacgcatc aggtgggcgt
     1681 tgacagcgcc gtgtgcatgc ctgtactcat tgcgatgaga tgggggatgg acaaatggag
     1741 cagcagaacc ttgtaaagct aagggccgtc aagcacggcg attccggcat cgcgctggag
     1801 ttcactggag tgaccggaga tatcggcaag tacttcgccg cgcagcccga cggcggcgag
     1861 ctgccgctcg aatacgtgga ggcgatcctt cgcttctatc tgaccaacag cagcggccgt
     1921 cagggcgtcg atcccggcgg caacgccccg gttcacgcgc tttggtgagc ggggcctcgc
     1981 actcgtggcc gccctgatgc ccaagcgctc cgtggagaag ccgaccaccg tcgattgtgc
     2041 gatcatcggt ggtggagtgg cggggctcta cgccggctgg cgcctcgcca ccggcgccag
     2101 cgtcgcgcag agcacggacg ctccgggctc gctcgccgtg ttcgagcaca gcgagcgcct
     2161 gggcgggcgc ctggacaccg ggaccgtcgg cgggaccccg gtcgagttcg gcgcgatgcg
     2221 ctatctgccc gcgcagaagc tgctcgacgc gctgttgcac gccggcctgc ccggcctcag
     2281 cacgacccct ttcgtgagca cacagctgca gatcctgtat ctgcgcggtg tcttcatgcg
     2341 gcctctggcg tcgaccccga gcccgtatca gctcgtcggg gccgaaaagg acaaatctgc
     2401 gctcgagctg ctcgcggggg cgatcgaggc gctggtcccc ggcgcgctcg agctcacgcc
     2461 tgcgcagtgg gaggtcgcca cgacccggcc ggtttacgac ggctatgcgc tgtaccagct
     2521 cgggctgtgg aacgtgctgt cgatggtgct cagcaacgag gcctataagt acgtgttcga
     2581 cgcgctgggc atcgagtcgg tgctgagtaa ctggaacgcc gccgaggggc tgcagctgct
     2641 gacattcatc ctgcgcgcgc tcgcgggcgg cccgcccggg gcgtttcatc gcctcgtcga
     2701 cggctggtcg cggctcaccg atacgctggc cgcgcagctt agcgcccacg gcggagagat
     2761 ccatcgtcag cacaccctcg tcgccctgct cccgcagcac gggcacgagc tgtcggcccg
     2821 cgacgaggag tacgtcgacg tcgccgcgat caaggccgcg atcaccgact ccatcgcgtc
     2881 gcagggcggg gatctacacg cgtcccacgg catggtcacc ctggtg
//
LOCUS       DASWNJ010001475         2575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_519601, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001475 DASWNJ010000000
VERSION     DASWNJ010001475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519601"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VGB85_24545"
     CDS             <1..749
                     /locus_tag="VGB85_24545"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY0137284.1"
                     /translation="LLDAVPDEWFARTDPDAAYLRECAAIGWTLLGGAARPVSGDMAQ
                     NTYDDVVARLRVGLAADQPELRFQCATGLAELEGAAAEDALAQALARETIPTIRGALV
                     GALGEIDPPSPRTCDVLAPLCDEPHTPTAFAAALALTVARDPRAGQQLLAALGRPEDR
                     GRALEALAALGPAAPPEALPAVRRLARSFFTPGLTKVRAAYALARLAPREGEAILERL
                     STALRGSVREAVTDARAGLAQLAARDAARG"
     gene            761..1576
                     /locus_tag="VGB85_24550"
     CDS             761..1576
                     /locus_tag="VGB85_24550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY0137285.1"
                     /translation="MATPTPTPTIPGLVDTHCHLDYSPIRDDLDATLARAAAAGVEQL
                     VHIACKPDAWTSAVALIEAHHQVHAVLGVHPHEATLADEPTLATLADLLHHPRVVAVG
                     ETGLDYFYDRSPREVQQHAMARQLELARAANKPIVLHIRDAHADAWAVVDAQAAVPGI
                     IHCFTGTPDEARQWLDRGYHLSFSGIATFPSAPALREAARICPLDRILLETDAPFLAP
                     VPMRGRKNEPAFIAFTCANLAQLRGEAPEALAGAAAANARALLGLPAPPPPHG"
     gene            1703..2377
                     /gene="rplC"
                     /locus_tag="VGB85_24555"
     CDS             1703..2377
                     /gene="rplC"
                     /locus_tag="VGB85_24555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEY0137286.1"
                     /translation="MDFQLGLIAKKIGMTQIFDADGTRVPVTVLHVAGNVVTAHRTSE
                     RDEYTALQVGYQEKKESRANKAELGLFKKAGVTPRQEIREFRVSPARLGEFPVGGELS
                     AAVFAEGSLVDVSGTSKGKGFQGVIKRHNMEGEKNSHGQHEFFRHGGSIGCRKTPGRV
                     HKGKRMTGHMGDERVTVQNLKVARIDVENGLVLVRGPIPGANNCFVTIRQAIKPAIRA
                     NQNTGA"
BASE COUNT          390 a         1069 c          782 g          334 t
ORIGIN      
        1 cgctgctcga cgccgtgccc gacgagtggt tcgcccgcac cgaccccgac gccgcctacc
       61 tgcgcgagtg cgccgcgatc ggctggaccc tgctcggcgg ggccgcccga cctgtctccg
      121 gggacatggc ccaaaatacc tacgacgacg tcgtcgcccg cctgcgcgtc ggcctcgccg
      181 ccgaccagcc cgagctgcgc tttcagtgcg ccaccggcct cgccgagctc gagggcgccg
      241 ccgccgagga cgcgctcgcc caggccctcg cccgcgagac gatcccgacg atccgcggcg
      301 cgctggtcgg cgccctcggg gagatcgatc caccgagccc gcgcacctgc gacgtgctcg
      361 ccccgctgtg cgacgagccg cacacgccga ccgccttcgc cgccgcgctc gccctcaccg
      421 tcgctcgcga cccccgcgct ggccaacaat tgctggccgc cctcggccgc ccggaggacc
      481 gcggccgcgc gctcgaggcc ctcgccgcgc tcggccctgc tgccccgccc gaggccctgc
      541 ccgccgtacg ccggctcgcc cgcagcttct tcacccccgg cctgacgaag gtccgggccg
      601 cctacgcgct cgcccgcctg gccccgcgcg agggcgaggc gatcctcgag cgcctctcga
      661 ccgccctgcg tggcagcgtg cgcgaggcgg tcaccgacgc ccgcgccggc ctcgcccagc
      721 tcgccgcccg cgacgccgcc cgcggctgaa aaaaaccacc atggccaccc cgaccccgac
      781 cccgacgatc ccgggcctcg tcgacaccca ctgtcacctc gactacagcc cgatacgcga
      841 cgacctcgac gcgacgctcg cccgcgctgc cgcggccggc gtcgagcagc tcgtgcacat
      901 cgcctgcaag ccggacgcct ggacctcggc ggtcgccttg atcgaagcgc atcaccaggt
      961 ccacgccgtg ctcggcgtcc acccgcacga ggcgaccctc gccgacgagc cgacgctcgc
     1021 gaccctcgcc gacctgctgc atcacccccg ggtcgtcgcc gtcggcgaga ccggcctcga
     1081 ctatttttac gaccgctccc cccgcgaggt gcagcagcac gcgatggccc ggcagctgga
     1141 gctcgcccgc gccgcgaaca agccgatcgt gctgcacatc cgcgacgccc acgccgacgc
     1201 gtgggccgtc gtcgacgccc aggccgccgt cccgggcatc atccactgtt ttaccggcac
     1261 ccccgacgag gcccggcagt ggctcgatcg cggctatcac ctgtcgtttt cggggatcgc
     1321 aaccttcccg tcggcgccgg ccctgcgtga ggcagcgcgc atctgccccc tcgaccgcat
     1381 cctcctcgaa accgacgccc ccttcctcgc cccggtgccg atgcgcggcc gcaagaacga
     1441 gccggccttc atcgccttca cctgcgcaaa tctggcccag ctccgcggcg aggctccgga
     1501 ggccctcgcc ggcgccgcgg ccgccaacgc ccgcgctctg ctcggcctgc cggccccgcc
     1561 tcctccccat ggttgacagc cgcgcccgcc cgttctaaac gggtcggccc ccgcgcacct
     1621 tgctcttcga ccaccaacgc gaaggaagcc gtgacgcggg gtttctcggt ccttcgggac
     1681 cactgtcaga caggaaacga tcatggattt tcagctcggg ctcatcgcca aaaagattgg
     1741 tatgacgcag atcttcgacg ccgacggaac gcgggtcccc gtgaccgtgc ttcacgtcgc
     1801 cggcaacgtc gtcaccgccc accgcacctc ggagcgtgac gagtacaccg ccctccaggt
     1861 cggctatcag gagaagaagg agtcgcgggc caacaaggcc gagctcggtc tcttcaagaa
     1921 ggccggcgtc accccccgcc aggagatccg cgagtttcgc gtctccccgg ctcgcctcgg
     1981 cgagttcccg gtcggcggcg agctgagcgc ggccgtgttc gccgagggca gcctcgtcga
     2041 cgtgtccggc accagcaagg gcaagggctt ccagggcgtc atcaagcgcc acaacatgga
     2101 aggcgagaag aacagccacg gtcagcacga gttcttccgc cacggcggat cgatcggctg
     2161 ccgcaagact cccggccgcg tccacaaggg caagcgcatg accggccaca tgggcgacga
     2221 gcgcgtcacc gtgcagaacc tcaaggtcgc caggatcgac gtcgagaacg gcctcgtgct
     2281 ggtccgcggc ccgatccccg gcgccaacaa ctgcttcgtg acgatccgcc aggcgatcaa
     2341 gccggcgatc cgcgccaacc agaacaccgg cgcctgagcg cctcgacctg tcctcgcgga
     2401 caggtcgtag agacccagaa gaacaggcag gcccacggcc tgcctttcgc gttccagcca
     2461 cctgccacct gccacttctc atgtcccgtc cgaagctcag cgacaaggcc acccgccacg
     2521 acgcggccta cctccgggcc aagcgcgaga actacgccgc gcgcgcgatc tacaa
//
LOCUS       DASWNJ010001476         2049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_96785, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001476 DASWNJ010000000
VERSION     DASWNJ010001476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96785"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..1088
                     /locus_tag="VGB85_24560"
     CDS             270..1088
                     /locus_tag="VGB85_24560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137287.1"
                     /translation="MRMLPRRSRLPIALVAVVCLVRPLPAGAAPAPLPDWGVKDKGKK
                     PADTAKPAEAKPADKPGTAPLPDWGVAGKPKAKKPKGKSKGKGKSSAETTPEETVEPT
                     EATEPAADETAPATPAEPAPEPAPEPAPEAVAPAPEPDEPAVTKPANDTTAAEQLRRR
                     GRAELITGAVFMAAGLTGFGVLGAGVFIKRSADQELEDAEGRTDVDLDPLYAQQKQGE
                     TMLAAGAVAGVIGMALGIALVGAGARDMKRGRAGLSSRVRVTPALGGLVISGRF"
     gene            complement(1104..>2049)
                     /locus_tag="VGB85_24565"
     CDS             complement(1104..>2049)
                     /locus_tag="VGB85_24565"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY0137288.1"
                     /translation="AAVVAVSMLRGDESTATKTATAQTPGKEVVATPATAEAPVASRC
                     PEGMALIPGGKFFMGSDNVDSPALLSARPAHRAEVADFCMDIHEVTVAEHTACSTTGE
                     CKRAHRDSFWPQGKSDKKSWEASRAAYNPLCNEGQQGRERHPANCVTWEQADHYCRAH
                     DKRLPSEAEWEFAARGSDGRLYPWGDEPPDAVHLNGCGRECVAWRAAAKLPEVPVLYD
                     ADDGHPGTAPVGSFPAGKTEAGLLDMVGNVFEWTADDFKPYPGAPAGAPVAEGAKVMR
                     GGGFNSSQAEHAEPALRFPQDATAHVHAVGFRCARAPL"
BASE COUNT          274 a          785 c          727 g          263 t
ORIGIN      
        1 cagcgcgacg aacatcgtca tcactcgaac ggcccggccc atcgcgcagc gcatacgccg
       61 ggtggtgtgc cacaacttcg cgtcgggcgt ttcaattccc gcgatcgtcc ggccgtccgc
      121 gtcggcgcac gatcggccag cgccgcgcgc gcgatcgtgt ccccggccgc ggggatccgc
      181 ggtggcgggg acagtgtaag ctctcgtcgc gccgggccgc cgcggcgcga ccctctcgcc
      241 agtttggacg tacggagcgg accacggcca tgcgtatgct tcctcgcaga tctcgcctcc
      301 cgatcgcact cgtggcggtc gtctgtctcg tgcgcccgct gcccgccggc gccgcccctg
      361 ctcccctccc cgactggggc gtcaaggaca agggcaaaaa accggccgac acggccaagc
      421 ccgccgaagc caagcccgcc gacaagccag gcaccgcgcc gctgcccgac tggggcgtcg
      481 ccggcaagcc gaaggcgaag aagccgaagg gcaagagcaa gggcaagggc aagtcgtccg
      541 ccgagaccac ccccgaggag accgtcgagc cgaccgaggc gaccgagccc gccgccgacg
      601 agacggcccc cgcgacgccc gccgagcccg ctccggagcc cgctccggag cccgcgcccg
      661 aggccgtcgc gcccgcgccc gagcccgacg agccggccgt cacgaagccc gcgaacgaca
      721 cgaccgccgc ggagcagctg cgtcgtcgcg gccgcgccga gctgatcacc ggcgccgtgt
      781 tcatggccgc cggcctgacc ggcttcggcg tgctcggggc cggcgtgttc atcaagcgca
      841 gcgccgatca ggagctcgag gacgccgagg gtcgcaccga cgtcgacctc gatcccctgt
      901 acgcccagca aaagcagggc gagacgatgc tcgccgccgg cgcagtggcc ggtgtgatcg
      961 gcatggcgct gggcatcgcc ctcgtcggcg ccggtgcgcg cgacatgaag cgaggccgcg
     1021 ccggcctgag ctcgcgagtc cgcgtcacgc cggccctcgg cggcctcgtg atctccggcc
     1081 gcttttaaaa aatcaccacg cgctcacagc ggcgcgcggg cgcagcggaa gccgaccgcg
     1141 tgcacgtgcg ccgtcgcgtc ctgcgggaag cgcagcgccg gctcggcgtg ctccgcctgc
     1201 gagctgttga agccgccgcc gcgcatcacc ttcgcgccct cggccaccgg cgcgccggcg
     1261 ggggcgcccg ggtagggctt gaagtcgtcg gcggtccact cgaacacgtt gccgaccatg
     1321 tcgagcaggc cggcctcggt cttgcccgcc gggaagctgc cgaccggcgc ggtgccgggg
     1381 tggccgtcgt cggcgtcgta gagcaccggc acctcgggca gcttggcggc agcgcgccag
     1441 gcgacgcact cgcggccgca gccgttgagg tgcaccgcgt cgggcggctc gtcgccccac
     1501 gggtacaggc ggccgtcact gccccgcgcc gcgaactccc actcggcctc gctcggcagg
     1561 cgcttgtcgt gcgcgcggca gtagtggtcg gcctgctccc acgtgacgca gttggcgggg
     1621 tggcgctcgc ggccctgctg gccctcgttg cacagcgggt tgtaggcggc gcggctggcc
     1681 tcccacgact tcttgtcgga cttgccctgc ggccaaaagc tgtcgcgatg ggcgcgcttg
     1741 cactccccgg tggtcgagca cgcggtgtgc tcggcgacgg tgacctcatg gatgtccata
     1801 caaaagtcgg cgacctcggc gcggtgcgcc gggcgtgccg agagcagcgc gggcgagtcg
     1861 acgttgtcgg accccatgaa aaatttcccg ccggggatca gcgccatgcc ctcggggcag
     1921 cggctcgcga ccggcgcctc ggccgtcgcc ggcgtcgcga cgacctcctt gccgggcgtc
     1981 tgcgcggtcg ccgtcttcgt cgccgtcgac tcgtcgccgc gcagcatcga caccgcgacc
     2041 accgccgcg
//
LOCUS       DASWNJ010001477         2318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_695995, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001477 DASWNJ010000000
VERSION     DASWNJ010001477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695995"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(272..1303)
                     /locus_tag="VGB85_24570"
     CDS             complement(272..1303)
                     /locus_tag="VGB85_24570"
                     /inference="COORDINATES: protein motif:HMM:NF039450.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADYC domain-containing protein"
                     /protein_id="HEY0137289.1"
                     /translation="MVFAALCAATLAGACDDVETRVQPDPTEPITERGLVGNDLLLNS
                     ATLNGWSLNGFRTNGWSLNGWSLNGWSLNNIQLVGSAFSGVKMIDGQAITLAGEDMIG
                     AELSLSNNGEPFTLRFDDIFINPADPDGDVYFYKVSVLDPVAGTWSSLCRDSYGEPTE
                     AIALRHHWNPDTGARIDDDVAVTLACRGAALAKCVEWGYRPWASVQHCTTNGCAQVDL
                     ADHHQACTRMARADYCGDGVPHTLNNTPIDVYDRLNPRVQAQASSGKEWKVEAEWGPD
                     GALCVGKELRVDMFAALGIAHTAPPCRAALQAVKDCGKFPASRAASSKLGNNYCPKWK
                     TEPNKCVIP"
     gene            complement(1455..>2318)
                     /locus_tag="VGB85_24575"
     CDS             complement(1455..>2318)
                     /locus_tag="VGB85_24575"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY0137290.1"
                     /translation="ELPHASQVPADFVYVPPGRFLFGSASDEDLRKGFFTAPPLHERR
                     TEAFLVARHETTYADWIAYLEALPEDLRAARMKLGEGSAFRQAPVLSRDDRGQWRISL
                     QMGEHKFSAVAGEPLRISTRTGASAVQDWQRFPVGGINWDDAQSYVRWLDETGRVPGA
                     RLCSEVEWERAARGADGRKFPTGDTIAPDDANFDETHARDFAAMGPDVVGAHPASASP
                     FGLHDTMGNVWEWVTAGDGAGSGAGPVARGGAFFFDKLTGSPMNRNVLDPGFRDGTLG
                     LRVCATPRFPT"
BASE COUNT          313 a          816 c          829 g          360 t
ORIGIN      
        1 gagcgtggtc ctggcagcga gcgtgggtcc gcgggcgagc gtggccccgc cagcgagcgg
       61 ggccccgcca gcgagcgtgg ccccgctggc gacgacgcga ccggatcggc gcctcgcaaa
      121 cgtcggcgac gtcgctccgg cagcggagcg ggtggcgcgg cgtcgggcgc cccgccagcg
      181 acgggctccg gctcacgcgc ctgagggtcg acctgtttct tcgagcggcc cggcgggcgg
      241 acctgtccct tcggtcagct ccgctcgcgc ctcaggggat cacgcacttg ttcggctcgg
      301 tcttccactt cgggcagtag ttgttgccga gcttgctgga agcggcgcgt gacgccggga
      361 acttgccgca gtccttgacg gcctgcagcg cggcgcggca cggcggcgcg gtgtgggcga
      421 tgccgagggc ggcgaacatg tcgacgcgca gctccttgcc gacgcacagc gcgccgtcgg
      481 ggccccactc ggcctcgacc ttccactcct tgcccgagct cgcctgggcc tggacccgcg
      541 ggttgaggcg gtcgtagacg tcgatcggcg tgttgttgag ggtgtgcggc acgccgtcgc
      601 cgcagtagtc ggcgcgggcc atgcgggtgc acgcctggtg gtggtcggcg aggtcgacct
      661 gcgcgcagcc gttggtggtg cagtgttgca cgctcgccca cgggcggtag ccccactcga
      721 cgcacttggc cagcgcggcg ccgcggcacg cgagggtgac cgcgacgtcg tcgtcgatgc
      781 gcgcgccggt gtccgggttc cagtggtggc gaagcgcgat cgcctcggtc ggctcgccgt
      841 agctgtcgcg gcacaggctc gaccacgtgc cggcgacggg gtcgagcacc gagaccttgt
      901 agaaatacac gtcgccgtcg gggtcggccg ggttgatgaa gatgtcgtcg aagcgcagcg
      961 tgaacggctc gccgttgttg gacagcgaca gctcggcgcc gatcatgtcc tcgccggcga
     1021 gcgtgatcgc ctgaccgtcg atcatcttca cgccgctaaa cgcgctgccg accagctgga
     1081 tgttgttgag gctccagccg ttgaggctcc agccgttgag gctccagccg ttggtgcgaa
     1141 agccgttgag gctccagccg ttgagggtgg ccgagttgag caggaggtcg ttgccgacga
     1201 gaccgcgctc ggtgatcggc tcggtcgggt cgggctggac ccgcgtctcg acatcatcac
     1261 acgcgccggc gagcgtcgcg gcgcacagcg cggcaaaaac catggatcgc gtctcggtcg
     1321 acatactcgt caccatcccc ggtttcgtct ttggaccctg tccgcggccg atgcaagcgc
     1381 cgcgaaacag gccttaaacg ccaagccgcg ccccgaaact cgcgtacacg ctgcttcgca
     1441 cgaagctcgt ggcgttacgt tgggaatctt ggcgttgcac agacgcgaag cccgagcgtg
     1501 ccgtcacgaa agccaggatc gaggacgttg cggttcatcg ggctgccggt cagcttgtcg
     1561 aagaagaacg ccccgccgcg cgccacgggg cccgcgccgc tgcctgcgcc gtccccggcg
     1621 gtcacccact cccagacgtt gcccatcgtg tcgtgcagcc cgaacgggct cgcgctcgcc
     1681 gggtgcgcgc cgacgacgtc ggggcccatc gccgcgaagt cgcgcgcgtg ggtctcgtcg
     1741 aagttggcgt cgtccggcgc gatcgtgtcg ccggtcggga acttgcgacc gtccgcgccg
     1801 cgggccgctc gctcccactc gacctcgctg cacagccggg cccccggcac ccgcccggtc
     1861 tcgtcgagcc agcgcacgta gctctgcgcg tcgtcccagt tgatcccgcc gaccgggaag
     1921 cgctgccaat cttgcaccgc gctcgcgccc gtgcgcgtcg agatccgcag cggctcgccg
     1981 gcgacggcgc taaacttgtg ctcgcccatc tgcagcgaga tccgccactg gccccggtcg
     2041 tcgcgcgaca gcaccggcgc ctggcgaaac gccgagccct cgccgagctt catgcgagcg
     2101 gcccgcagat cctccggcaa agcctcgaga tacgcgatcc agtcggcgta ggtggtctcg
     2161 tggcgggcga ccaggaacgc ctcggtgcgc cgctcgtgca acggcggcgc ggtgaagaag
     2221 cccttgcgca ggtcctcgtc gctcgcgctg ccgaacagga accgccccgg tggcacatac
     2281 acgaagtcgg cgggcacctg cgaggcgtgc ggcagctc
//
LOCUS       DASWNJ010001478         1747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_519681, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001478 DASWNJ010000000
VERSION     DASWNJ010001478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519681"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VGB85_24580"
     CDS             complement(<1..194)
                     /locus_tag="VGB85_24580"
                     /inference="COORDINATES: protein motif:HMM:NF021456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HEY0137291.1"
                     /translation="MWNLVNLTPFAADRCFARDPTGLEYFVVALKATFDLRPDGSLQV
                     TGAQQPIAYAPVYRGEPARS"
     gene            complement(195..>1747)
                     /locus_tag="VGB85_24585"
     CDS             complement(195..>1747)
                     /locus_tag="VGB85_24585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137292.1"
                     /translation="LLDAAHHRGVRLGVRFSNPNDPTNKDEPPHRKGAKDQFDDILGG
                     IFWSGMEGQRDQFSIAGRVSAPMIEARNAGMGIELTPLFWLFGFAGDNIEVWKKSKKK
                     TKSGKVKVTWKQAAPRPIEWGPNPGWARGITDVNEAMGCIKNFITRLVTGLDQLDPEH
                     PIVPHVKRWVVINEPMNILMRDVTRTAPFLNTVMPTVPDPNHAKKDMKKLKHQHDRPP
                     VLVKLAPQAIDNMVAILQHAHVTIPNDSLLIVNDYGIEGYDEKGGLTGLRGFRFRELM
                     QGILDRVKDPALLKRLRVGLQGHLNSGGELDTGSKTKAASARKELESRGERNDFRVSS
                     VRKQMQWFKSKGLRVCVTECDLVLPTVTYEVKVPGKKKLKTLEALDEHIRLGTHVVTE
                     NGKPKIKKKPGFSAHKFFNSKLRRRPYLDAFAKQRIQQRDLINAILGSGNVDSFSWWD
                     FDDDLPEDHAGSNFRYHGYLFHEHLVCKHCKNHPAMQVADETGILYKKPSYWGTVAAF
                     LNSSGSGF"
BASE COUNT          264 a          515 c          605 g          363 t
ORIGIN      
        1 ctcgagcggg ccggctcgcc gcgatatacc ggcgcgtacg cgatcggctg ctgggcgcct
       61 gtgacctgca gcgagccgtc ggggcgcagg tcgaaggtcg ccttgagcgc gaccacgaag
      121 tattcgagcc cggtcgggtc acgcgcgaag cagcggtccg cggcgaaggg ggtgagattg
      181 acgaggttcc acatctagaa gccgctgccg gaggaattca ggaacgcggc gacggtgccc
      241 cagtaggacg gctttttata taaaattcca gtctcgtcgg cgacctgcat cgcggggtgg
      301 tttttgcaat gtttacatac caggtgctcg tggaacaggt atccgtggta acgaaagttg
      361 gacccggcgt ggtcctcggg caggtcgtcg tcgaagtccc accaggagaa cgagtcgacg
      421 ttgccggagc cgaggatcgc gttgatcagg tcacgctgct ggatccgctg cttcgcaaac
      481 gcatcgaggt aggggcggcg ccgcagcttg ctgttgaaga atttgtgggc gctaaacccg
      541 ggcttcttct tgatcttcgg cttgccgttc tcggtgacca cgtgcgtgcc gagcctgata
      601 tgttcgtcga gggcctcgag cgtctttagc ttcttcttgc ccgggacctt gacctcgtag
      661 gtgacggtcg ggagcacgag atcgcactcg gtgacgcaga cgcgcaggcc cttgctcttg
      721 aaccattgca tctgcttgcg cacggaggac acgcgaaagt cgttgcgctc gccgcgggac
      781 tcgagctcct tcctggcgct ggcggccttg gtcttgctgc cggtgtcgag ctcgccgccg
      841 gagttgaggt ggccctggag gccgacgcgc agccgcttga gcagggcggg gtccttgacg
      901 cggtcgagga tgccctgcat gagctcgcgg aagcgaaacc cccggagtcc ggtcaggccg
      961 cctttttcat cgtagccctc gatcccgtag tcgttgacga tcaggaggga gtcgttcggg
     1021 atcgtcacgt gggcgtgttg caggatcgcc accatgttgt cgatcgcctg cggcgcgagc
     1081 ttgaccagca ccggcgggcg gtcatgctgg tgctttagct tcttcatgtc ctttttggcg
     1141 tggttcgggt cggggaccgt cggcatgacc gtgttgagga acggggccgt gcgcgtcacg
     1201 tcgcgcatga ggatgttcat cggctcgttg atcacgaccc agcgcttgac gtgcgggacg
     1261 atcgggtgct ccgggtcgag ctggtcgagc ccggtgacca gccgggtgat gaaattcttg
     1321 atgcacccca tcgcctcgtt cacgtcggtg atgccgcgcg cccagcccgg gttggggccc
     1381 cattcgatcg gcctgggggc ggcctgcttc caggtgacct tgaccttgcc tgacttggtc
     1441 ttcttcttcg acttcttcca gacctcgatg ttgtcgccgg cgaagccgaa cagccagaag
     1501 agcggggtca actcgatgcc catgccggcg ttgcgggcct cgatcatcgg cgcgctgacc
     1561 cggccggcga tcgagaactg gtcgcgctga ccctccatgc cggaccagaa gatgccgccg
     1621 aggatgtcat cgaactgatc cttggcgccc tttcggtgcg ggggctcgtc cttgttggtg
     1681 gggtcgttgg ggttgctaaa gcgcacgccg agccggacac cgcgatggtg agcggcgtcg
     1741 agcagct
//
LOCUS       DASWNJ010001479         1544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_338378, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001479 DASWNJ010000000
VERSION     DASWNJ010001479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338378"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VGB85_24590"
     CDS             <1..363
                     /locus_tag="VGB85_24590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137293.1"
                     /translation="FNGDQEEEFKWFARALTSRWTATSTDYAARLVVDAAIHGDRQRA
                     VSPGSWLLSRLHGLAPSLMTHFWSRFDRHLPPPGPPGTLTKMHLGTEVLADSPDGEVQ
                     ARGEAARLDARRYRPPGS"
     gene            454..873
                     /locus_tag="VGB85_24595"
     CDS             454..873
                     /locus_tag="VGB85_24595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137294.1"
                     /translation="MDRLQIVRDVFPISGELGSLELVDPDPYAEPDRLTIRLLVWERL
                     PDGERQIRDIKEQQVWVGGLEDVDPERLTQAVHGWAMALAQLFAQDDVTWMETLMPAD
                     LMPNLASLVALRRPRSAEDFAEAMLSSKQRLGRWLSP"
     gene            complement(863..>1544)
                     /locus_tag="VGB85_24600"
     CDS             complement(863..>1544)
                     /locus_tag="VGB85_24600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0137295.1"
                     /translation="RGFQDVADDTHNIVRSAHHLLTIINDILDLSKIEAGKLDMVVED
                     FDVAALTQEVAETIAPMISDYQNTLVVRIEAVDTRMRSDRTKLRQTLLNLLSNAAKFT
                     ERGKIELRLRHETVAGVPCHILSVRDEGVGIPEGALATLFDPFTQVVYPQVRKRAGTG
                     LGLAITRRLCWMMGGEIDVESVSGKGSTFTVWIPSVYRSLGSNESWRPLRKRGVTKHS
                     GPLPTFKG"
BASE COUNT          211 a          536 c          552 g          245 t
ORIGIN      
        1 ttcaacggcg accaggagga ggagttcaag tggttcgcgc gcgccctgac ctcgcgctgg
       61 acggcgacga gcaccgacta tgccgcgcgc ctcgtcgtcg acgctgcgat ccacggcgac
      121 cgccagcggg ccgtctcgcc gggctcgtgg ctgctctcgc gcctgcacgg gctcgcgccc
      181 tcgctgatga cccacttctg gtcccgcttc gaccgccacc tgccgccgcc gggccccccg
      241 ggcacgctca cgaagatgca cctcggcacc gaggtcctcg ccgacagccc ggacggcgag
      301 gtccaggcgc gcggcgaggc ggcccgcctc gacgcccgcc gctaccgccc gccgggctcg
      361 tgaccacggg gcatgggggc atgggcatgg gcatgtgccg gctcacttgt atgtgcgccg
      421 gcccgcgctt gatggcggtt tatcgtcgcg ggcatggacc gcctgcagat cgtccgcgac
      481 gtgttcccga tctcgggtga gctcggatcg ctcgagctcg tcgaccccga tccatatgcg
      541 gagcccgacc gcttgacgat ccggctgctg gtctgggagc ggctgccgga cggcgagcgg
      601 cagatccgcg acatcaagga gcagcaggtg tgggtcggcg gcctcgaaga cgtcgacccg
      661 gagcggctca cccaggcggt ccacggctgg gccatggcgc tcgcgcagct gttcgcccag
      721 gacgacgtga cctggatgga gaccctgatg cccgccgacc tgatgccgaa cctcgcgtcg
      781 ctcgtcgcgc tccggcggcc gcgcagcgcc gaggacttcg ccgaggcgat gctgtcgagc
      841 aagcaacggc tcgggcgctg gctcagccct tgaacgtcgg cagcggcccg gagtgcttgg
      901 tgacgccccg cttgcgcagc ggtcgccacg actcgttgct gccgaggctg cggtagacgg
      961 aggggatcca gaccgtgaag gtcgagccct tgccggacac gctctcgacg tcgatctcgc
     1021 cgcccatcat ccagcacagg cgccgcgtga tcgccaggcc gaggccggtg ccggcgcgct
     1081 tgcggacctg cggatacacg acctgcgtga acgggtcgaa cagggtcgcc agcgcgccct
     1141 cggggatccc gacgccctcg tcgcggacgc tgaggatgtg gcacggcacg ccggcgaccg
     1201 tctcgtggcg caggcgcagc tcgatcttgc cgcgctcggt gaacttggcg gcgttgctga
     1261 gcaggttgag cagcgtctgg cgcagcttgg tgcgatcgct gcgcatgcgg gtgtcgacgg
     1321 cctcgatgcg gacgaccagc gtgttctgat agtcgctgat catcggcgcg atcgtctcgg
     1381 cgacctcctg cgtcagcgcg gcgacgtcga agtcctcgac gaccatgtcg agcttgccgg
     1441 cctcgatctt cgacaggtcg aggatgtcgt tgatgatcgt cagcaggtga tgcgccgagc
     1501 ggacgatgtt gtgggtgtcg tcggcgacgt cctggaagcc gcgc
//
LOCUS       DASWNJ010001480         2468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_557828, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001480 DASWNJ010000000
VERSION     DASWNJ010001480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557828"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1465)
                     /locus_tag="VGB85_24605"
     CDS             complement(<1..1465)
                     /locus_tag="VGB85_24605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HEY0137296.1"
                     /translation="MWTPPEKHRHPVAPTRWPTAEEAAGSRWFSPLACGPLLLEQRTW
                     VPAMVPWRASEDGLVTRDVIDWYGRFAAGRPGAIVVEATGIRDVPSGPLLRIGDDRFV
                     PGLRDLVAAVKSASDGHTRLLIQLIDFLAVKRRPPADKFFGRFLNVDDGHRARLIVAT
                     GDPRWRDAPEPELRSALLALGPGTWEHVLTDRERRDLEYGYREQVSDLDLPHVRQLPE
                     VLPGLFAAAAARAQEAGFDGVELHYAHAYTMASFLSRTNTRTDGYGGAREGRVRLPLE
                     VIAAVRREVGQGFALGCRMLGDEVIAGGSDLDDAVWFARAFARAGLDFISVSKGGKFD
                     DAEAPKVGEAVYPYTGPSGYECMPTVYSDARGPFARNVQLASAIRAGIRGEGLTTPVV
                     AAGGFNSFEQAEQALADGQADVVAAARQSLADPDWFLKVAMGRGEEVRRCKFTNYCEA
                     LDTKHKQVTCQLWDRKDMDAPDVTLSHDGKRRLLAPRW"
     gene            1622..>2468
                     /locus_tag="VGB85_24610"
     CDS             1622..>2468
                     /locus_tag="VGB85_24610"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEY0137297.1"
                     /translation="MDIAAFPADEAERMAALRGYAVLDTPPEQEFDELTQFASALCDT
                     PIALVSLVDTERQWFKSRVGLDVPETSRNLAFCSHAILQRGIFEVEDASRDKRFADNP
                     LVASDPNIRFYAGAPLLHPSGYQLGTLCVIDRVPRKLTPIQRQGLEVLSRQVISQLEL
                     RRQVRQLARLSLEIAESRDQALAGTRAKDIFLASMSHELRTPLNAILGLSQFLLERDE
                     LPADATADLHTIDRSGRHLLAVIEDILGYAQLELERPQLRARAFDLGELLGEVEAGVR
                     PQLRGR"
BASE COUNT          360 a          934 c          827 g          347 t
ORIGIN      
        1 gccagcgcgg cgccaataac cgccgcttgc cgtcgtgcga cagcgtgacg tccggcgcgt
       61 ccatgtcctt gcggtcccac agctgacatg tcacctgctt gtgcttggtg tcgagcgcct
      121 cgcagtagtt cgtgaacttg cagcgccgca cctcctcccc gcgccccatc gctactttca
      181 agaaccagtc ggggtcggcg aggctctggc gggccgccgc gacgacgtcg gcctgcccgt
      241 ccgcgagcgc ctgctcggcc tgctcgaagc tgttgaagcc gcccgcggcg acgaccggcg
      301 tggtgagccc ctccccccgg atccccgcgc ggatcgccga agccaattgc acgttgcggg
      361 cgaagggccc gcgcgcgtcg ctgtacaccg tcggcatgca ctcgtagccg ctcgggccgg
      421 tgtacgggta caccgcctcg ccgaccttcg gcgcctcggc gtcgtcgaac ttgccgccct
      481 tcgacacgct gatgaagtcg aggcccgccc gcgcgaacgc ccgcgcgaac cacaccgcgt
      541 cgtcgaggtc gctgccaccg gcgatcacct cgtcgccgag catgcggcag ccgagcgcga
      601 agccttgccc gacctcgcgc cgcaccgccg cgatcacctc gagcggcagc cgcacccgac
      661 cctcgcgcgc gcctccgtag ccgtccgtgc gtgtgttggt gcgcgagagg aagctcgcca
      721 tcgtgtacgc gtgcgcgtag tgcagctcga ccccgtcgaa gcccgcctcc tgcgcccgcg
      781 cggccgcggc cgcgaacaac cccgggagca cctccggaag ctggcgcacg tgcggcaggt
      841 cgaggtcgga gacctgctcg cggtagccgt actcgaggtc gcgtcgctcc ctgtccgtca
      901 ggacatgttc ccaggtacct ggcccgaggg ccaggagcgc gctgcgcagc tccggctcgg
      961 gcgcgtcgcg ccagcgcgga tctccggtgg cgacgataag ccgcgcgcgg tgcccgtcat
     1021 ccacatttaa aaaccgcccg aagaacttgt cggcgggcgg gcggcgcttg accgcgagga
     1081 agtcgatcag ctggatgagc aggcgcgtgt ggccatcgct ggcggacttc accgcggcga
     1141 cgagatcccg caggccgggc acgaagcggt cgtcgccgat ccgcagcagc ggccccgagg
     1201 gcacgtcgcg gatccccgtc gcctcgacga cgatcgcgcc cggtcgcccc gccgcgaagc
     1261 ggccgtacca gtcgatcacg tcacgcgtca ccaggccgtc ttcgctcgcg cgccacggca
     1321 ccatcgccgg cacccacgtg cgctgctcga gcagcagcgg tccgcaagcg aggggcgaga
     1381 accaccgact gccggcagcc tcctcggcgg tcggccagcg cgtcggcgcg acggggtggc
     1441 gatgcttctc cggcggagtc cacatgagcg cggggccgaa cacattacca cgcggtcgca
     1501 cgaccgccga acccgtgtgc ctgaccgcat tcggccactg ctaccgaagc tgccagaggt
     1561 caccggagaa ttccgccaga acttcgttgc ggctcgtggg acatccctta cgctgggatg
     1621 catggacatc gctgcgttcc ccgccgatga ggccgagcgg atggccgcgc tgcgtggcta
     1681 cgcggtcctc gacacgcccc ccgagcagga gttcgacgag ctgacgcagt tcgcctcggc
     1741 gctgtgcgac acaccgatcg cgctggtgag cctggtcgac accgagcggc agtggttcaa
     1801 gtcgcgggtc ggcctcgacg tgccggagac ctcgcgcaac ctcgcgtttt gcagccacgc
     1861 gatcttgcaa cgcgggatct tcgaggtcga ggacgcgtcg agagacaagc gcttcgccga
     1921 caacccgctg gtcgccagcg accccaacat ccggttctac gccggcgccc cgctgctgca
     1981 ccccagcggc taccagctcg gcacgctgtg cgtcatcgac cgcgtgccgc gcaagctgac
     2041 gccgatccag cgccagggcc tcgaggtgct cagccgccag gtgatcagcc agctcgagct
     2101 gcggcgacag gtccgccagc tcgcgcgcct gagcctggag atcgccgagt cgcgcgacca
     2161 ggcgctcgcc ggcacgcggg ccaaggacat cttcctcgcc agcatgagcc acgagctgcg
     2221 caccccgctc aacgcgatcc tcggcctgag ccagttcctg ctcgagcgcg acgagctgcc
     2281 cgcggacgcg accgccgacc tgcacacgat cgaccgttcg ggccggcacc tgctcgcggt
     2341 gatcgaggac atcctcggct acgcgcagct cgagctcgag cggccgcagc tgcgcgcgcg
     2401 ggccttcgat ctcggcgagc tgctgggcga ggtcgaggcc ggcgtgcgcc cgcagctgcg
     2461 cgggcgcc
//
LOCUS       DASWNJ010001481         4455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_203707, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001481 DASWNJ010000000
VERSION     DASWNJ010001481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203707"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(416..1882)
                     /locus_tag="VGB85_24615"
     CDS             complement(416..1882)
                     /locus_tag="VGB85_24615"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HEY0137298.1"
                     /translation="MSSQTTSGRWISPDSGPVVARTFHAALGLVFVIAFASLASQIDL
                     LFAAGGLAPIAETTELLREAEIDPWLAPSLFWLSASDTALYTAAIAGMLCGLLALLGR
                     LVRPALALATILYLAFVPLGAPFLGFQWDVLLLECGMLAVLLPRGRAAPVVHLLLAFL
                     LFKLYFESGVAKWYSPLGDWHDGSAMTVYYETAPIPTRLAWYAHHMPASWHALEGWVV
                     LLGELLVPLLIFAGRRGRLIAFFALTGFQLVNLLTANYGFFIHLALALHLFLLRDEDL
                     ERPRQWLARLPSWLRAPAPEDSSPGRLRTTLTATFAIIYLALSVLDARANFSDSETAR
                     AWQARFAPLYAPLSMINTYHLFAAITRERIEPEFSATADDTTWTDYALHYKPGPLDRP
                     PPFVAPHQPRLDFQLWFYGLAAESRPPGYILRLLVMLCRDPERAQAAFATPLLPDPAA
                     IRLRFWRYRFSPPGSPSWWTREQVGESGSLRCAELPDA"
     gene            complement(1879..2733)
                     /locus_tag="VGB85_24620"
     CDS             complement(1879..2733)
                     /locus_tag="VGB85_24620"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin-like protein"
                     /protein_id="HEY0137299.1"
                     /translation="MPALELRHLAPILLLVACFQDGPENDTGSSGAPVTTTGPADTST
                     GTPTTGGSTTDASTTSTGSSTDASSDTGSSSGTSTGPACMTPWYRDFDEDGHGDPDVS
                     MMACMRPDGHVGLGDDCDDMDAARAPGFAEKCDDKDNDCDVLIDEYSPQNVLCKQCSL
                     FARGDHSYAFCTFLRSWPDARTECKKRSGDLAVIDDAEENAALAAQGTITPNTNGGWY
                     IGLSDLAAEGTFVWLDGLPLGFAGWNLGEPNDAGMAEDCTLLPNTGLWNDQGCEPPAH
                     FLCESPAP"
     gene            2879..>4455
                     /locus_tag="VGB85_24625"
     CDS             2879..>4455
                     /locus_tag="VGB85_24625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY0137300.1"
                     /translation="MRLTLDPFRTSLLAALGLIACGPIDKTSGDEGGSDSSGAASTSG
                     AGDTTAVTSATDPTTGATETTQGSATSGQTGGQTDTGKTTDGSTSSATTDTTGDDTGA
                     VSCPSEPVPALQGFTDPPVPSGFERCEMGILHRAEKLACDVPQTPSTCLDNMLGGCTS
                     DADCVEKPFGSCQQDMVFGGFTEGSCSCVYGCQTDDDCAAGEICRCAGDGLGLYTQCI
                     AADCTVDSDCPDGEICGLSPDACAPGGFLTACTTPADTCDDNSECPQPGCMFELDHWV
                     CSNIACGRPFVVEAQAVVAPLQARDDWRALLATPEIDAALAPRLAEYWANIGQLEHAS
                     VAAFAQFVLQLLAVGAPPTLVLAAQRALADEVEHARLCFGLASLYKKNAGVGPGPLAI
                     SGAVAPADLATLVDAVIREACIGETLAAFEAREAAAQAEDPVVRAVLGKIAADEARHA
                     ELGWQFVQWALVDDAAVARARATFESAIAEARRGVERDAQAVGAPELRAHGVVDAPLR
                     AEVWAEGLRAVVEPCAAA"
BASE COUNT          659 a         1456 c         1731 g          609 t
ORIGIN      
        1 gcgccttcgc cgggatgata ggcgctcgcc tcgcgacacc gcgccgcggc cctgcgtgcc
       61 ggctgtgatc ctgcacaccc acgcgcgagc atccgcgatc gctagcgcgc ggggcgatgc
      121 acgtcgtcgc gcggccgcac gcggggaggg tgggcgatcg gttcacatcc cggcatggag
      181 ctgtccgcgt ggctgatcgc gctcccgatc accgaaccga gcgcctgctc gcggccggcg
      241 tcccgctgtc gcgcttcatg atcggctcaa cacgatgcaa ccggagatcg tcatccgcca
      301 caacccgcac gcgcgccggt accggccgta ctacctcgcc agcccgacgg ctcgcgccgg
      361 tgaccagcgc gacgcggcca cgcagcgaga ggtggacatc ggggtctctc tcgtttcagg
      421 cgtccgggag ctcggcgcag cgcaggctgc cggactcgcc gacctgctct cttgtccacc
      481 acgagggact accgggcggg ctgaatcgat aacgccagaa gcgcaggcgg atcgcggcgg
      541 gatcggggag cagcggggtg gcgaaggcgg cctgggctcg ctcggggtcg cggcagagca
      601 tcacgagcag gcgcaggatg tagccggggg gtctgctctc ggcggcgagg ccgtaaaacc
      661 acagctggaa gtcgaggcgg ggctgatgcg gggcgacgaa ggggggaggg cgatcaagag
      721 ggccgggctt gtaatggagg gcgtagtcgg tccaggtcgt gtcgtcggcg gtggcggaga
      781 attcgggctc gatgcgctcg cgggtgatcg ccgcgaacag atgataggtg ttgatcatgc
      841 tcagcggggc gtacaggggg gcgaacctcg cctgccaggc gcgcgccgtc tcgctgtcgg
      901 agaagttggc gcgcgcgtcg agcacggaga gcgcgagata aatgatcgca aacgtggccg
      961 tcagggtcgt gcgcaggcga cctggagagc tgtcctcggg ggcaggtgcg cggagccatg
     1021 aaggaagccg ggcgagccac tggcgcgggc gctcgaggtc ctcgtcgcgc agcaggaaca
     1081 ggtgcagggc gagggcgagg tggatgaaga agccgtagtt ggcggtcagc aggttgacga
     1141 gctggaagcc ggtcagcgcg aagaaggcga tcaggcggcc gcggcggccc gcgaagatca
     1201 gtagagggac gagcagctcg ccgagcagca cgacccagcc ctcgagggcg tgccagctcg
     1261 cgggcatgtg gtgcgcgtac caggcgaggc gcgtcgggat cggcgcggtc tcgtaataaa
     1321 ctgtcatcgc gctgccgtcg tgccagtcgc cgagcggcga gtaccacttg gcgacgcccg
     1381 actcgaagta gagtttgaac aagaggaacg cgagcagcag gtgcacgacc ggcgcggcgc
     1441 ggccgcgggg cagcagcacc gccagcatgc cgcactcgag cagcagcacg tcccactgga
     1501 acccgaggaa gggggcgccg agcgggacga aggcgaggta gaggatcgtc gccagcgcga
     1561 gggccgggcg cacgaggcgg ccgagcaggg cgagcaggcc gcacagcatg ccggcgatcg
     1621 cggcggtgta gagggcggtg tcgctggcgc tcaaccagaa caggctcggc gcgagccagg
     1681 ggtcgatctc ggcctcgcgc agcagctcgg tggtctcggc gatcggcgcg aggccgccgg
     1741 cggcgaacag caggtcgatc tggctggcga gcgaggcgaa ggcgatcacg aacacgaggc
     1801 cgagcgcggc gtgaaaggtg cgcgcgacca ccgggccgct gtcgggggag atccagcgac
     1861 cactcgtcgt ctgcgagctc atggcgccgg cgactcgcac aggaaatggg ccggaggctc
     1921 acagccctgg tcgttccaca ggccggtgtt gggcagcagc gtgcagtcct cggccatgcc
     1981 ggcgtcgttg ggctcgccga ggttccagcc ggcgaagccg agcggcaggc cgtcgagcca
     2041 cacgaaggtg ccctcggccg cgaggtcgct gaggccgatg taccagccgc cgttggtgtt
     2101 cggcgtgatc gtcccctggg ccgcgagcgc ggcgttctcc tcggcgtcgt cgatcactgc
     2161 gaggtctccg ctgcgcttct tgcactcggt gcgcgcgtcg ggccagctgc gcaagaaggt
     2221 gcagaacgcg tagctgtggt cgccgcgggc gaacaggctg cactgcttgc acaggacgtt
     2281 ctgcggcgag tactcgtcga tcaatacgtc gcagtcgttg tccttgtcgt cacacttctc
     2341 ggcgaagccg ggtgcgcggg cggcgtccat gtcgtcgcag tcgtcgccga gtccgacgtg
     2401 accgtccggg cgcatgcacg ccatcatcga gacgtcgggg tcgccgtggc cgtcctcgtc
     2461 gaagtcgcgg taccacgggg tcatgcacgc cgggcccgtg ctcgtgccgc tgctggatcc
     2521 ggtgtcactc gaggcgtcgg tgctcgagcc ggtgctcgtg gtgctcgcgt cggtggtcga
     2581 gccgccggtg gtcggggtgc cggtgctcgt gtcggcgggg ccggtggtcg tgacgggggc
     2641 gccggagctg ccggtgtcgt tctccgggcc gtcctggaag cacgcgacga gcagcaagat
     2701 cggtgcgagg tgacggagtt cgagtgcggg catcgcgggg gcggccagcg tatcgcacgc
     2761 ggacgggggc tggccaccgc gcttcgcggg cgccgctgcg cgtggcggcc ggcaggacga
     2821 tgttccccgg gcacccatgt ggcttgaccg cgaggcgcgg cgggtgtctc gtcagtggat
     2881 gcgacttacc cttgaccctt ttcgtacatc cctcctggcc gcgctcgggc tgatcgcctg
     2941 cgggccgatc gacaagacca gcggcgacga aggcggcagt gacagcagcg gcgcggccag
     3001 caccagcggc gctggcgaca cgaccgcggt gaccagcgcg accgacccga ccaccggcgc
     3061 gaccgagacc acgcagggct ccgccaccag cgggcagacc ggcggacaga ccgacaccgg
     3121 gaagaccacg gacggctcga cctcgagcgc gaccaccgat accacgggcg acgacaccgg
     3181 cgcggtgagc tgcccgagcg agccggtgcc tgcgctgcag ggcttcaccg acccaccggt
     3241 accctcgggc ttcgagcgct gcgagatggg gatcttgcac cgcgcggaga agctcgcgtg
     3301 cgacgtgccg cagacgccga gcacctgcct cgacaacatg ctcggtggtt gcacgagcga
     3361 cgcggactgc gtcgagaagc cgttcggttc gtgccagcag gacatggtgt tcggcggctt
     3421 cacggagggc agctgctcgt gcgtgtacgg ctgccagacc gacgacgact gcgctgcggg
     3481 cgagatctgc cggtgcgcgg gcgacgggct cggtctgtac acgcagtgca tcgcggcgga
     3541 ctgcaccgtc gacagcgact gccccgacgg cgagatctgc gggctgtctc ccgacgcctg
     3601 cgcgccgggc ggcttcctca cggcatgcac cacgccggcc gacacctgcg acgacaacag
     3661 cgagtgcccg cagccgggct gcatgttcga gctcgaccac tgggtctgct ccaacatcgc
     3721 gtgcgggcgg ccgtttgtgg tcgaggcgca ggcggtggtc gccccgctgc aagcgcgcga
     3781 cgactggcgc gcgttgctgg cgacgcccga gatcgacgcc gcgctcgcgc cgaggctcgc
     3841 cgagtactgg gccaacatcg gccagctcga gcacgcctcg gtcgccgcgt tcgcgcagtt
     3901 cgtgttgcag ctgctcgcgg tcggggcccc gccgacgctg gtgctcgcgg cccagcgggc
     3961 gctcgccgac gaggtcgagc acgcgcgcct gtgcttcggc ctggcgagcc tttataagaa
     4021 gaatgccggg gtcggcccgg gtccgctggc gatctccggg gccgtcgcgc cagcggacct
     4081 ggccacgctc gtcgacgcgg tgatccgcga ggcgtgcatc ggcgagacgc tcgcggcctt
     4141 cgaggcgcgc gaggccgccg cgcaggccga ggaccccgtc gtgcgcgcgg tgctggggaa
     4201 gatcgcagcg gacgaggcgc ggcacgccga gctcgggtgg cagttcgtgc agtgggcgct
     4261 cgtcgacgac gctgcggtcg cgcgcgcgcg ggccacgttt gaaagtgcga tcgcagaggc
     4321 gcgccgcggc gtcgagcgtg acgcgcaggc tgtaggtgcg ccggagctgc gggcccacgg
     4381 ggtcgtcgac gcgcccctgc gcgccgaggt ctgggcagag ggcctgcgcg cggtcgtcga
     4441 gccgtgcgcg gcggc
//
LOCUS       DASWNJ010001482         3106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_165970, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001482 DASWNJ010000000
VERSION     DASWNJ010001482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165970"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VGB85_24630"
     CDS             <1..703
                     /locus_tag="VGB85_24630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137301.1"
                     /translation="PRTLMCMPAIDGVRRHVQNPMSLAAWKFEAFFDMLTVEDALLES
                     LVEATALPETPVMVRGDDGSPEVWLVDGVMGEYRRHVPDPKVAAAWRLDLATVTTVPA
                     GDIEVLMMGPPLRPRPFLIKGTGPAVYVLDDPLPVPGGESTGGSEGTSGAPEGSTGPG
                     ATSDVPTTTMTMTPTSTAGDTGTGGTGGGSGEESTGPAVDDGDAGCGCDTRGGTPGAA
                     LALLLLGLRRRRVAR"
     gene            710..1045
                     /locus_tag="VGB85_24635"
     CDS             710..1045
                     /locus_tag="VGB85_24635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137302.1"
                     /translation="MAKKPGKAKYPETPDGRYFVVRGRLWRRANPGLTEEEREALVRE
                     LMTARRAVQAALKSGEGLAAARMKVDVAKRGLGERGPVWWTDGAPDLNRKMVKNTPYA
                     EWFAALDDP"
     gene            complement(1050..1883)
                     /locus_tag="VGB85_24640"
     CDS             complement(1050..1883)
                     /locus_tag="VGB85_24640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0137303.1"
                     /translation="MSTAIPRVDPEDGQSVALLQQVLDTIPDPIFVKDLEHRWIAVND
                     AFCRLIGHPREALLGRSDPDFWPPEQTAVFWHNDDLVFRSGESNENEETATGADGVER
                     TLWTRKIPLRDAADRVTGLCGVVTEITALKQRMREAERIERENAEHRGLIAAQAAMLA
                     ELTMPVVEVGDRILLVPLIGELSQRRVATANEEMLASIARLRARFVLFDMTGVPTIDT
                     AVARDLLRTLDAAALLGCRGALCGIRPEIAQMLVALDVKLGAFQTFATLRAGLAYASR
                     T"
     gene            complement(1973..2548)
                     /locus_tag="VGB85_24645"
     CDS             complement(1973..2548)
                     /locus_tag="VGB85_24645"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137304.1"
                     /translation="MSTTPDSACDPSPWLQDISSASPPRTSCSRPTWPPPRSPDALRF
                     PMIVKHPNSYGSVGMTRDARVTDRAKLATQSARLLDEFGGVLIEELIEGRELTVLVAE
                     NPDAPREPVAFVPAETPGSTASTSSPATRPDHPRPRDPVRRGPRALHLPLRIFRLPRP
                     DPRQRLLVACGPRALRRARHRLHPPKARAAS"
     gene            2644..>3106
                     /locus_tag="VGB85_24650"
     CDS             2644..>3106
                     /locus_tag="VGB85_24650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137305.1"
                     /translation="MDIALSVTLGLLFLVTAFVAVFLMFHLWGYPFDKVARKSEAPPG
                     LMRLHRVLGYVYVVLYLVMMTEMVPRLWTYQVELPARTVMHICFGLLIGTVLILKISI
                     IRWFRHLEEWMPVLGVLLLSFTLLLTGLSVPFALREYALARSAAGGSVYSEE"
BASE COUNT          443 a          929 c         1248 g          486 t
ORIGIN      
        1 cccgcgcacg ctgatgtgca tgccggcgat cgacggcgtg cgccgccacg tgcagaatcc
       61 gatgagcctg gcggcctgga agttcgaggc gttcttcgac atgctgacgg tcgaggacgc
      121 gctgctcgag tcgctggtcg aggcgactgc cctgccggag acgccggtga tggtgcgcgg
      181 cgacgacggc agcccggagg tgtggctggt cgatggcgtg atgggcgagt atcgccggca
      241 tgtgcccgac ccaaaggtcg ctgcggcgtg gcggctcgat ctcgcgacgg tgacgacggt
      301 gccggccggc gacatcgaag ttttgatgat ggggccaccg ctgcggccgc ggccgttttt
      361 gatcaagggg acagggccgg cggtgtatgt gctcgatgac ccgctgccgg tgccgggcgg
      421 ggagagcacc ggcgggtcgg aggggacgag cggggcgccg gaggggtcca cggggccggg
      481 cgcgacgtcg gatgtgccga cgacgacgat gaccatgacg ccgacgagca ccgccgggga
      541 taccgggact ggtggcaccg gaggtggctc gggtgaggag agcacggggc cggcggtcga
      601 cgatggcgac gcgggctgtg gttgtgatac gcggggcggg acacccggcg ctgcgctggc
      661 gctgctgttg ctggggctgc ggcggcgccg ggtggcgcgc tgaggggaca tggccaaaaa
      721 gccaggtaaa gcgaagtacc ccgagacgcc ggacgggcgc tacttcgtgg tgcgcgggcg
      781 gctgtggcgg cgcgcgaacc cggggctgac ggaggaggag cgcgaggcgc tggtgcggga
      841 attgatgacg gcccggcgcg cggtgcaggc ggcgctgaag tcgggcgagg ggctggcggc
      901 ggcgcgcatg aaagttgatg tcgccaagcg tgggctcggc gagcgcggac cggtgtggtg
      961 gaccgacggg gcgccggatc tcaatcgcaa gatggtgaag aacacgccgt atgcggagtg
     1021 gttcgcggcg ctcgacgatc cgtgacgtgt caggtgcgcg aggcgtatgc caggccggcg
     1081 cgcagggtgg caaaggtctg gaaggcgccg agcttgacgt cgagggcgac gagcatctgg
     1141 gcgatctccg ggcggatccc acacagggcg ccgcggcagc cgagcagggc ggcggcgtcg
     1201 agggtgcgca gcaggtcgcg ggcgacggcg gtgtcgatcg tcggtacgcc ggtcatgtcg
     1261 aacaggacga agcgggcgcg gagcctggcg atgctggcga gcatctcctc gttcgcggta
     1321 gcgacgcggc gctggctgag ctcgccgatc agcggcacca gcaagatgcg gtcgccgacc
     1381 tcgacgaccg gcatcgtcag ctcggcgagc atggccgcct gggcggcgat caggccgcga
     1441 tgctcggcgt tctcgcgctc gatccgctcc gcctcgcgca tgcgctgctt gagcgccgtg
     1501 atctcggtga cgaccccgca caggccggtc acgcggtcgg cggcgtcacg cagggggatc
     1561 ttgcgggtcc agagcgtgcg ctcgacgccg tcggcaccgg tcgcggtctc ctcgttctcg
     1621 ttgctctcgc cggaccggaa cacgaggtca tcgttgtgcc agaacacggc ggtctgctcg
     1681 ggcggccaga agtcggggtc gctgcgcccg agcagggcct cgcgcggatg gccgatcagg
     1741 cgacaaaacg cgtcgttgac ggcgatccag cggtgctcga ggtccttgac gaagatcggg
     1801 tcggggatcg tgtcgagcac ctgctgcagc agcgcgacgg actgcccgtc ttcggggtcg
     1861 acccggggga tcgcggtgga catcggagtg agattatatg cgcggcgcag cggccggaat
     1921 ggccgcttac ggggtcctcg tttgggcccg acggcagacg cagacgcggc gatcaggagg
     1981 ccgcgcgcgc tttcggcgga tgtagtcggt gacgtgctcg ccgtagcgct cgcggaccgc
     2041 aggcaacaag tagtcgctgc cgcggatcag gccgcggcag tcggaagatc cgcagcggca
     2101 ggtgaagggc tcgaggccct cggcgaacag ggtcgcgtgg tcgagggtga tctggccgcg
     2161 tggcgggcga cgaggtcgag gccgtcgaac caggcgtttc ggccggcacg aaggcgaccg
     2221 gctcgcgcgg ggcgtccggg ttctcggcga cgagcacggt gagctcgcgg ccctcgatga
     2281 gctcctcgat cagcacgccg ccgaactcgt cgagcaggcg tgcagactgg gtggccagct
     2341 tggcgcggtc ggtgacgcgg gcgtcgcggg tcatgccgac gctgccgtag ctgttggggt
     2401 gtttgacgat catcgggaag cgcagcgcgt ccggcgagcg cggcggtggc caggtcgggc
     2461 gcgaacatga agttcggggc ggcgatgccg aactgatgtc ctgcaaccag ggcgaggggt
     2521 cgcaggcgct gtccggggtg gtcgacaccg agtggagcac gcagatccgc acggaatttt
     2581 cgtgcgcccg gggcggctgg gccgtcaagg cgtgcggggt tttgctaagg tcgcggggct
     2641 cgcatggaca tcgccctcag cgtcaccctc ggcctcctgt tcctggtcac ggccttcgtg
     2701 gccgtgtttc tgatgttcca cctgtggggc taccccttcg acaaggtggc ccgcaagagc
     2761 gaggcccccc cgggattgat gcgcctacac agggttctcg gctacgtgta cgtggtcctg
     2821 tacctggtga tgatgacgga gatggtcccc cgtctgtgga cctaccaggt cgagctaccc
     2881 gcccgcacgg tgatgcacat ctgcttcggc ctgctgatcg ggacggtgct gatcctcaag
     2941 atctcgatca ttcgctggtt ccgccacctc gaggagtgga tgccggtgct cggggtgctg
     3001 ctgctgtcgt tcacgctgct gctcacgggc ctgtcggtgc cgttcgcgct gcgggagtac
     3061 gccctggcgc gctcggcggc gggtggcagc gtctacagcg aggaga
//
LOCUS       DASWNJ010001483         2054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_96937, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001483 DASWNJ010000000
VERSION     DASWNJ010001483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96937"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VGB85_24655"
     CDS             <1..634
                     /locus_tag="VGB85_24655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0137306.1"
                     /translation="PLQDLIQEGNIGLMKAVDRFDHRKGFRFSTYGSWWIRHAISRSI
                     ADKARAVRLPVHMIDAYNKVCRARRDFEALHGRKPTELELSTATGVSTERLQRMNYSL
                     VENPVSLDQPLSSDSGLTLMDAVEDTQTPEAAELMDNELLMNNLREVFAALSPMEADI
                     LRKRMGLGEEQELTLKEIGERYSLSRERIRQLQEQALGKMRDEFKRRALL"
     gene            complement(827..1102)
                     /locus_tag="VGB85_24660"
     CDS             complement(827..1102)
                     /locus_tag="VGB85_24660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137307.1"
                     /translation="MSEVDVHRLALEKVGRILGPARASNLLTTFLSAWPPRERLQSPE
                     DLYAFGRCLSEMQGMEQAVGALLTVQAVILGAPGEPGNSGTYGRGKL"
     gene            1286..>2054
                     /locus_tag="VGB85_24665"
     CDS             1286..>2054
                     /locus_tag="VGB85_24665"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY0137308.1"
                     /translation="MSLPRRPALGLRGLPAPDGGVLVTAIDAGTAAAAILRAGDRLLA
                     IDDLPVDDPRMIAVQLRGRRSGDDVTLRFVRDGEPAEGRLTLAPLPEETCPEAHVHYD
                     SIEVAGVRLRTITARPSAATGPVPAALLLPDLACASQDFALAPGQPAQAIVAALAAAG
                     VASMRVERPGLGDSEGGPCEALGWRDEVAMFRAGLAALASDPAVDPDALCLFGHGVGG
                     TLAPIVGAEERARRIVVFGTCARRWSACVRRSAGPADR"
BASE COUNT          276 a          811 c          682 g          285 t
ORIGIN      
        1 gccgctgcag gacctgatcc aggagggcaa catcggcctg atgaaggcgg tcgaccgctt
       61 cgaccaccgc aagggctttc gcttctcgac ttacggcagc tggtggatcc gtcacgccat
      121 ctcgcgctcg atcgccgaca aggcccgcgc cgtgcgcctg ccggtccaca tgatcgacgc
      181 ctacaacaag gtctgccgcg cccgccgcga cttcgaggcc ctgcacggcc gcaagccgac
      241 cgagctcgag ctctccaccg cgaccggcgt gtcgaccgag cgcctccagc ggatgaacta
      301 ctcgctcgtc gagaaccccg tcagcctcga ccagcccctg tcctccgact ccggcctgac
      361 cctgatggac gccgtcgagg acacccagac ccccgaagcc gccgagctga tggacaacga
      421 gctgctgatg aacaacctcc gcgaggtgtt cgccgccctg tcgccgatgg aggccgacat
      481 cctgcgcaag cgcatgggcc tcggcgagga gcaggagctg accctcaagg agatcggtga
      541 gcgctactcg ctgtcccgcg agcgcatccg ccagctccag gagcaggccc tcggcaaaat
      601 gcgcgacgag ttcaagcgcc gcgccctgct gtagtcgact cgactcgagg ccccgaaagc
      661 ggcctctcac attcgaggcc ccttccgggg cctcactcgc tcgcggctcc gaaaggggcc
      721 tctcgcactc gaggcccgaa ggcccgctcg ctcgcggctc cgaaagcggc ctctcgcgct
      781 cgaggccatc cgcgtcgcgc tcgcggctcc gaagggcctc actcgtttac agcttgccgc
      841 gcccgtaggt cccggagttt ccgggctctc cgggcgcgcc gaggatcacg gcctggaccg
      901 tcagcagcgc cccgacggcc tgctccatcc cctgcatctc cgacaggcag cgcccgaacg
      961 cgtacaggtc ctccggcgac tgcaggcgct cgcgcggcgg ccaggccgac aggaaggtcg
     1021 tcagcaggtt cgacgcccgc gccggtccga ggatccgtcc gaccttctcg agcgcgaggc
     1081 gatggacgtc gacctcgctc atgcggtccc ccgcggcgag ggatccccgt cgcccgctgg
     1141 cgcgttcgcg ccgcgagctg gtggggatct atgctcgaag gtggtcatcg cctccccact
     1201 gtgccgggat gcgagggtcg gcgcaaatgc ggccgacagc ccccacgggc ccgaatgccg
     1261 acattcgcct gttattctgc gcgcgatgtc cctgcctcgc cgccccgctc tcggccttcg
     1321 cgggctgccc gccccggacg gcggcgtcct cgtcactgcg atcgacgccg gcaccgccgc
     1381 cgccgcgatc ctgcgcgccg gtgatcgcct gctcgcgatc gacgatctgc ccgtcgacga
     1441 cccacgcatg atcgccgtcc agctccgcgg ccgccgcagc ggcgacgacg tcacgctgcg
     1501 cttcgtccgc gacggcgagc cggccgaggg ccgcctgacg ctcgcaccgc tgcccgaaga
     1561 gacatgtccc gaagcacatg tccattacga cagcatcgag gtcgccggcg tccgcctgcg
     1621 cacgatcacc gcgcggccca gcgccgcgac cggcccagtc cccgcggcgc tgctgctgcc
     1681 cgatctcgcc tgcgccagcc aggacttcgc gctcgcgccg ggccagcccg cgcaggcgat
     1741 cgtcgccgcg ctcgcggccg ctggcgtcgc gtcgatgcgg gtcgagcggc cgggcctcgg
     1801 ggacagcgag ggcggcccct gcgaggcgct cggctggcgc gacgaggtcg cgatgttccg
     1861 cgccggcctg gccgcgctgg cgagcgaccc cgcggtcgac cccgacgcgc tgtgcctgtt
     1921 cgggcacggc gtcggcggca cgctcgcgcc gatcgtcggc gccgaggagc gcgcgcgccg
     1981 gatcgtcgtg ttcggaacct gcgcccggcg ctggtccgcg tgcgtgcgcc gctcggccgg
     2041 ccccgcggac cgcg
//
LOCUS       DASWNJ010001484         2921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_448411, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001484 DASWNJ010000000
VERSION     DASWNJ010001484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448411"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VGB85_24670"
     CDS             <1..635
                     /locus_tag="VGB85_24670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137309.1"
                     /translation="WAVRLFGKDVRQLVASRVFRAPASAVLDALAQVWQASPYNLRLL
                     DAVGPHPLDGGAVMFRVPKFSLGTDGSSVGFLYYMHVLAIHQVRLTMQRRGGDAVECT
                     VIVELHEAIAFGRRLFYPWMIGMTTVAGGAAGAALFVAMMPILAALTTLLTVATVYGG
                     LYKLNHVTYRYYIKTAQEHLEALLADVDAKLRTQAVFGSLPAPRPGPPIF"
     gene            complement(857..2164)
                     /locus_tag="VGB85_24675"
     CDS             complement(857..2164)
                     /locus_tag="VGB85_24675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137310.1"
                     /translation="MEVKTIILSALVAFGAVALVSQIDLRRGSTAAPVATRATETATG
                     PGYAALLASIDERVQGLKGRTEARADDWLGRMHLGTALFERAGLTNQVEDYARVQAVL
                     DDAFAIAPQGSGPLLLAARFNFSIHRLAGAEKYLDMLDRRAVPRGDEQLVARVLRAEI
                     AVQRGQYDEAFKALTEVAAVDPTLANTALALYHAKTGKPAEAATLLEATLAGTRENDP
                     QRRAWLRLQLGILEMDRGELQAAQEHLEAADAELRGWWLVHEHVAELHSRRDEHDKAI
                     AIYEELVRTADLPQHMDALASLYRHLGEPQKAAALISRAAARWEQQLARFPESAMGHA
                     LQHHLEFGSPERAVELALANHALRPGGEAQVSLARAHLGAGQLAEARAVVDRTLATPY
                     RTARLHDVAAKVYAALGDTAAAQAQISLRLAVNPRFTSDDHSH"
     gene            complement(2263..>2921)
                     /locus_tag="VGB85_24680"
     CDS             complement(2263..>2921)
                     /locus_tag="VGB85_24680"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HEY0137311.1"
                     /translation="TDPTSTTTPTTMTTPTTTTDPTTTTDPTVGPTTGEPDTTTGGAG
                     YVFPTDPIDSYVQIDRHGAVEAGTAGILASQGLGFKMGSDIKLRDAYNASNPEEDVAG
                     MWVPEIVKSIMFFHGALDDDIKALGLVPATTDDTVAQAGPVIIPDTIKYNPTMPSGYP
                     NGRKLEDQVVDLTLAAVLLKLGPKQPLTTFADLPLNPPANDVPFSAEWPYLAPPHPGK
                     "
BASE COUNT          369 a         1003 c         1077 g          472 t
ORIGIN      
        1 gctgggccgt gcgcctgttc ggcaaggatg tccgccagct cgtcgcctca cgggtgtttc
       61 gcgcgccggc gagcgccgtg ctcgacgcgc tcgcacaggt gtggcaggcc agcccttaca
      121 accttcgcct gctcgacgcc gtcggcccgc atccgctcga cggcggcgcg gtgatgttcc
      181 gcgtgccgaa gttcagcctc ggcaccgacg gctccagcgt tggatttctc tattacatgc
      241 acgtgctggc gatccaccag gtccgcctga cgatgcagcg ccgcggcggt gacgcggtcg
      301 aatgcacggt gatcgtcgag ctgcacgagg cgatcgcctt cggccgccgg ttgttctacc
      361 cgtggatgat cggcatgacc acggtcgctg gcggcgccgc cggggcggcg ctgttcgtgg
      421 cgatgatgcc gatcctcgcc gcactgacga cgctcctcac cgtcgccacg gtctacggtg
      481 gcctgtacaa gctcaaccat gtcacctacc gctattatat caagacagcg caggagcacc
      541 tcgaggcgct gctcgccgac gtcgacgcca aactgcgcac gcaggcggtg ttcggcagcc
      601 tgcccgcccc gcgaccggga cccccgatct tttagatatg cgtctcgggc ttcgcgtcga
      661 tcggtccgac gccgctgaca cgcgaagata acgatagtcc tcgtcggcgc gcagcaggcg
      721 atacggcggc ggaccgggca ctcccctgcc ccgccccgcg agcgcggccg ccattccatg
      781 ggagatccca gtcgtcggtc atcgctcctc ctcgcggcgc aatgtagcga accgcgcggg
      841 ctggccaccg tggtgatcag tgcgagtggt cgtcgctggt gaagcggggg ttgaccgcca
      901 ggcgcagcga gatctgggcc tgcgcggccg ccgtgtcgcc cagcgccgcg tacaccttgg
      961 cggcgacgtc gtgcaggcgg gccgtgcggt acggggtcgc cagggttcga tcgaccaccg
     1021 cgcgcgcctc ggcgagctgg cccgcgccca ggtgggcgcg cgccagcgac acctgcgcct
     1081 cgccgccggg gcgcagcgcg tggttggcga gcgccagctc gaccgctcgc tcgggtgaac
     1141 cgaactcgag gtgatgttgc agcgcgtggc ccatcgccga ctcggggaag cgggcgagct
     1201 gctgctccca gcgcgccgcg gcccgggaga tcagcgccgc ggccttttgc ggctcgccga
     1261 ggtggcgata cagcgacgcg agcgcgtcca tgtgctgcgg gaggtcggcg gtgcggacca
     1321 gctcctcgta gatcgcgatc gccttgtcgt gctcgtcgcg ccggctgtgc agctcggcga
     1381 cgtgctcgtg caccagccac cagccgcgca gctccgcgtc cgcggcctcg aggtgctcct
     1441 gcgcggcctg cagctcgccg cggtccatct ccaaaatgcc gagctgcagt cgcagccacg
     1501 cccggcgctg cgggtcgttc tcgcgggtcc ccgcgagcgt cgcttcgagc agcgtcgccg
     1561 cctcggcggg cttgccggtc ttcgcgtgat agagggcgag cgcggtgttc gccagcgtcg
     1621 ggtccaccgc ggcgacctcg gtcagcgcct tgaacgcctc gtcgtactgg ccgcgctgca
     1681 cggcgatctc ggcgcgcagg actcgcgcga ccagctgctc gtcgccccgc gggacggcgc
     1741 ggcggtcgag catatcgagg tacttctcgg cgcccgcgag gcggtggatc gagaagttga
     1801 agcgggccgc gagcagcagc gggccgctgc cctgcggcgc gatcgcaaag gcgtcgtcga
     1861 gcaccgcctg cacgcgggcg taatcctcga cttggttggt caggccggcg cgctcgaaca
     1921 gcgccgtgcc gaggtgcatg cggccgagcc agtcgtcggc gcgggcctcc gtgcggccct
     1981 tgaggccctg cacgcgctcg tcgatgctgg cgagcagcgc cgcgtagccg gggccggtcg
     2041 cggtctcggt cgctcgcgtg gcgaccgggg cggccgtgct gccgcgacgg aggtcgatct
     2101 gcgagacgag ggcgacggct ccgaacgcga cgagggcgga gagaatgatc gttttgactt
     2161 ccatgacgga cgatcggcga gtgggtcccc gaacgcgcga gcggcccccc gggagcttta
     2221 cacgcccggg gagccgtccg caaactcagc cgcgcgcgcg gctcacttgc cggggtgcgg
     2281 cggggccagg tacggccact ccgcggagaa cggcacgtcg ttcgcgggcg ggttgagcgg
     2341 caggtccgcg aaggtcgtca gcggctgctt cgggccgagc ttgagcagca cggcggcgag
     2401 ggtcaggtcg acgacctggt cctcgagctt gcgaccgttc gggtagccgg acggcatggt
     2461 ggggttgtac ttgatcgtgt ccgggatgat gaccgggccg gcctgtgcga cggtgtcgtc
     2521 ggtggtcgcg ggcacgaggc cgagggcctt gatgtcgtcg tcgagggcgc cgtggaagaa
     2581 catgatgctc ttgacgatct cggggaccca catgcccgcc acgtcctcct ccgggttgct
     2641 ggcgttgtag gcgtcgcgca gcttgatgtc cgagcccatc ttgaagccga ggccctgcga
     2701 ggccaggatg cccgccgtgc cggcctcgac cgcgccgtgg cggtcgatct gcacgtaaga
     2761 gtcgatcgga tcggtcggga acacgtagcc agccccgccg gtggtcgtgt cgggctcgcc
     2821 ggtggtcgga ccgaccgtcg ggtccgtcgt cgtcgtcggg tccgtcgtcg tcgtcggggt
     2881 cgtcatcgtc gtcggggtcg tcgtcgacgt cgggtccgtc g
//
LOCUS       DASWNJ010001485         3248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_338393, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001485 DASWNJ010000000
VERSION     DASWNJ010001485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338393"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VGB85_24685"
     CDS             <1..419
                     /locus_tag="VGB85_24685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137312.1"
                     /translation="DRNLITVRDLAGDAERSFELLHAHTQEGLEYTIARRAEEQSRDA
                     QHMLEAAHRLNMIAAVFLPVTAVATLLGMNLRHGLEDWPAPLTFWVTLVLCFVMGLWI
                     KASMPRPPAREPAPARSSAKPGAKAKPKGKGGLKGG"
     gene            complement(423..1319)
                     /locus_tag="VGB85_24690"
     CDS             complement(423..1319)
                     /locus_tag="VGB85_24690"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HEY0137313.1"
                     /translation="MSKRNPTLTIGLVQQSCSEDRDANLEASFAGVREAAAQGAELVL
                     LQELHTGVYFCQTEDTDRFDMAESIPGPSTEQIGQVARELGVVIVSSLFERRAPGLYH
                     NTAVVLERDGSIAGKYRKMHIPDDPGFYEKFYFTPGDLGFTPIDTSVGRLGVLVCWDQ
                     WYPEAARLMALAGAEVLLYPTAIGWEPRDEELERGRQREAWITVQRGHAIANGLPVVV
                     ANRVGHEPDPSGAAPGINFWGSSFITGPQGEFLAQAPVGEPAVLVTQVDRARSEKVRR
                     IWPYLRDRRIDAYEDLTRRYRD"
     gene            complement(1396..2235)
                     /locus_tag="VGB85_24695"
     CDS             complement(1396..2235)
                     /locus_tag="VGB85_24695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137314.1"
                     /translation="MRSFLGWGLLPLALACAFDPTGSQTTGAGTTSTGMDGVTGSTGP
                     ATGTSGVPTTTVAATSTGEPVVTSEPVTTSPTTSTSEPTTGEPGTTGGSTGGSTTEVD
                     TTGAPETTGPPCEPGFYKQVVLVADAVVEAPMVKVMSDMGEGMIAYSPTEGMGIASFT
                     FDLPCADEVAIWGRALDLMPGLNMTNDADSYWVSADDGAETGWFYGCQTLGQPAGYHW
                     LRVTSATLDQTCLAQPWTLSLPAGSHTIHLRNSEAASEQTVATIARLLITNDFNYVPV
                     APE"
     gene            complement(2305..>3248)
                     /locus_tag="VGB85_24700"
     CDS             complement(2305..>3248)
                     /locus_tag="VGB85_24700"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEY0137315.1"
                     /translation="VFVRIAVEVARREDVLIACRDSELAGRVRMLLRAAGGDLARVHL
                     HAVPTQDTWARDHGPITILRRDLPLLLDFGFNGWGGKFAADQDDQISRRLHALGAFDH
                     APIARIDLVLEGGSIETDGAGTLLTTRQCLLSATRSGLSEAEITAQLTALFGLERVLW
                     LGHGHLEGDDTDGHIDTLARFTDAATIAYQGCDDPADPHFAGLQAMAGELAALRTLGG
                     APYRLVPLPWPRARFSAEGERLPASYANFLIINDAVLVPTYDDPADAQALAALAACFP
                     GREVVGIDCLPVIEQYGSLHCLTMQLPQPLSLGPGTA"
BASE COUNT          437 a         1214 c         1131 g          466 t
ORIGIN      
        1 aggaccgcaa cctgatcacc gtgcgcgacc tcgccggcga cgccgaacgc agcttcgagc
       61 tgctgcacgc gcacacccag gagggcctcg agtacacgat cgcccgccgc gcggaggagc
      121 agtcgcgcga cgcccagcac atgctcgagg cggcgcaccg cctcaacatg atcgccgccg
      181 tgttcctgcc cgtcaccgcg gtcgcgaccc tgctcggcat gaacctgcgc cacggcctcg
      241 aggactggcc cgcgccgctg accttctggg tcaccctcgt gctgtgcttc gtgatgggcc
      301 tgtggatcaa ggcctcgatg ccgcgaccgc ccgcccgcga gcccgcaccc gcccgctcct
      361 ccgcaaagcc gggcgcgaag gccaagccca agggcaaggg cgggctcaag ggcggctgag
      421 ctttagtcgc ggtagcgccg ggtcaggtcc tcgtaggcgt cgatccggcg gtcgcgcagg
      481 tacggccaga tccgccgcac cttctcgctg cgcgcgcggt cgacctgcgt caccagcacg
      541 gcgggctcgc cgaccggcgc ctgggccagg aactcgccct gcggtccggt gatgaagctc
      601 gagccccaga agttgatccc cggcgccgcg ccgctcgggt cgggctcgtg gccgacgcgg
      661 ttggcgacga cgaccggcag cccattggcg atcgcgtgcc cgcgctggac ggtgatccac
      721 gcctcgcgct ggcggccgcg ctcgagctcc tcgtcgcgcg gctcccagcc gatcgccgtc
      781 gggtacagca gcacctccgc gcccgcgagc gccatcaggc gcgcggcctc cgggtaccac
      841 tggtcccagc acaccagcac gccgaggcga ccgaccgacg tgtcgatcgg cgtgaagccg
      901 aggtcgccgg gcgtgaagta gaatttttcg tagaagcccg ggtcgtccgg gatgtgcatc
      961 ttgcggtact tgccggcgat gctgccgtcg cgctcgagca cgaccgcggt gttgtgatac
     1021 agccccggcg cgcgccgctc gaacagcgag ctgacgatca cgacgccgag ctcacgggcg
     1081 acctgcccga tctgctccgt gctcggcccc gggatcgact ccgccatgtc gaagcggtcg
     1141 gtgtcctcgg tctggcagaa gtacacgccg gtgtgcagct cctgcagcag caccagctcg
     1201 gcgccctgcg cggccgcctc gcggaccccc gcgaacgagg cctcgaggtt ggcgtcgcgg
     1261 tcctcgctgc agctctgctg cacgaggccg atcgtcaagg tcgggttacg cttgctcacg
     1321 gctctcctat acgtccgcgc tccccgcggc gcaacgccgg ggcgcgccgc ggcgacgcgg
     1381 gcatgcgcac tgatgtcact ccggcgcgac cggcacgtag ttgaagtcgt tcgtgatcag
     1441 cagccgggcg atcgtggcga ccgtctgctc gctcgccgcc tcgctgttgc gcaggtggat
     1501 ggtgtggctg cccgcgggca ggctcagggt ccacggctgc gccaggcagg tctggtcgag
     1561 cgtcgccgag gtcacgcgca gccagtggta gcccgccggc tgcccgagcg tctgacagcc
     1621 gtagaaccac cccgtctcgg cgccgtcgtc ggcgctcacc cagtacgagt cggcgtcgtt
     1681 cgtcatgttg agccccggca tgaggtcgag cgcgcgcccc cagatcgcga cctcgtcggc
     1741 gcacggcaga tcgaaggtga agctggcgat gcccatcccc tcggtcggcg agtaggcgat
     1801 cataccctcg cccatgtcgc tcatcacctt gaccatcggc gcctccacga ccgcgtccgc
     1861 cacgagcacg acctgcttgt aaaagccggg ctcgcacggc gggcccgtcg tctccggggc
     1921 tcccgtggtg tccacctccg tggtcgagcc gcccgtcgag ccgcccgtcg tgcccggctc
     1981 gcccgtcgtc ggctcgctcg tcgaggtcgt cgggctcgtc gtcaccggct cactcgtgac
     2041 cacgggctcg cccgtgctcg tcgcagcgac ggtggtcgtc ggcacgccgg acgtgcccgt
     2101 ggctggcccc gtcgagcccg tgacgccgtc catgccggtg ctcgtggtgc cggccccggt
     2161 cgtctggctc ccggtcgggt cgaacgcgca ggcgagcgcg agcggcagca gaccccatcc
     2221 caggaacgat cgcatcgtcc aagcctaacg cagttcccct cgcccgtggc cgtgaccgag
     2281 cgagcgcaac cgagcatatc cgcgctacgc cgtacctggc ccgagggaca ggggctgcgg
     2341 cagctgcatc gtcaggcagt gcaggctgcc gtattgctcg atcaccggca ggcagtcgat
     2401 gccgaccacc tcgcgcccgg ggaagcacgc cgcgagggcc gccagcgcct gcgcgtccgc
     2461 cgggtcgtcg taggtgggca ccagcaccgc gtcgttgatg atcaggaagt tcgcgtagct
     2521 cgcgggcagg cgctcgccct cggccgagaa gcgggcccgc ggccatggca gcggcaccag
     2581 gcggtacggc gcgccgccga gagtccgcag cgccgcgagc tcgcccgcca tcgcttgcag
     2641 gcccgcgaag tgcgggtcgg ccgggtcgtc gcagccctga tacgcgatcg tggcggcgtc
     2701 ggtgaagcgg gccagcgtgt cgatgtgacc gtcggtgtcg tcgccctcga ggtgcccgtg
     2761 gccgagccac agcacgcgct cgagcccgaa cagcgccgtc agctgcgccg tgatctcggc
     2821 ctcggacagc ccgctgcgcg tcgccgacag caggcactgg cgggtcgtca gcagcgtgcc
     2881 ggcgccgtcg gtctcgatgc tgccgccctc gagcacgagg tcgatgcggg cgatcggcgc
     2941 gtggtcgaag gcgccgagcg cgtgcaggcg ccgcgagatc tggtcgtcct ggtcggccgc
     3001 gaacttgccg ccccagccgt tgaagccgaa gtcgagcagc agcggcaggt cccgccggag
     3061 gatcgtgatc ggcccgtggt cgcgggccca cgtgtcctgc gtcggcaccg cgtgcaggtg
     3121 cacgcgcgcg aggtcgccgc cggcagcgcg cagcaacatc cgcacgcgac cggcgagctc
     3181 gctgtccctg cacgcgatca acacgtcctc ccgccgcgcg acctcgacgg cgatccgcac
     3241 gaacaccg
//
LOCUS       DASWNJ010001486         3976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_623050, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001486 DASWNJ010000000
VERSION     DASWNJ010001486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623050"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VGB85_24705"
     CDS             complement(<1..621)
                     /locus_tag="VGB85_24705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137316.1"
                     /translation="MLRRSLALSLLLACSSTPPAGDGAGEDGSSDTGADASSGSATDP
                     PPDDPTGGTPSEEPMVACDPWLQDCPEGEKCTHYNTMGDNTVVDATKCVPLPAMPVPT
                     GGGCTATGGFGAGDDDCGRGDFCYFLDGDELGVCVPLCMGSAASPSCPDGSFCSHGLG
                     VALDLCLPACDAFEQNCLEGANCTPQEDGLVCALDTSGAGGQIYDAC"
     gene            720..1514
                     /locus_tag="VGB85_24710"
     CDS             720..1514
                     /locus_tag="VGB85_24710"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0137317.1"
                     /translation="MTGPAVGTLVRDWRTRRRRSQMDLALDVGVSPRHLSFVETGRAR
                     PSPELLLSLAEHLELPLRERNVLLLAGGYAPRYSETSLDAAAMQRVRAALQRMLDTHH
                     PYPGVVIDRCWNIMLANAAAGALLHGLPPALLGPPTNVFRVCLHPEGLAPRTRNLPEW
                     SAYLLGQLHRATLLTGDPALARLSEEVGRYPNLHAGPPRRDSDEPTLLVPVRLDVGGA
                     ELAMFTTLTTFGTPQDITLAELAVELFFPADAASEALLRGAPGPRA"
     gene            1588..2406
                     /locus_tag="VGB85_24715"
     CDS             1588..2406
                     /locus_tag="VGB85_24715"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0137318.1"
                     /translation="MPTLTTTDDTQLYYDDWGTGRPVVFVHGWSLCNASWDHQLPVFI
                     REGLRCVAPDRRGHGRSSRPGTGYDFDTLADDLAALLDRLDLREVTFVAHSMGAGEVV
                     RYLSRHGAGRVARIALLAPITPYLRKSPDNPHAVDAAQFEMLAAALGDDRAAWFAASA
                     PGFFGAGQPDCHASAALIDWGIRMILDTPLKVQLDTLHTHTSTDFRPDLAAVNVPTLV
                     IHGDIDQSAPIDLTGRPTAAQIAGARLEVYSGAPHGLMFTHQQRLHHDLLAFIR"
     gene            complement(2396..>3976)
                     /locus_tag="VGB85_24720"
     CDS             complement(2396..>3976)
                     /locus_tag="VGB85_24720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137319.1"
                     /translation="DLTLADLGSELVVAGAGVMARLGAGGALELMPDVQRGLIEPSNM
                     EGLGLLMLAGRWPDTVWMSVETYGERSSSNPLMYYRKGERWQRKATRVGLLEWFYGAY
                     APYTDGQVLGLRMMAPGTDLYEKYDGELPDSLQKKIDAATLANPPRLDVLAGGVMTPS
                     TMKLAAGATPIHFTALPTGEVFVLLASSVTTGEDESTTSFSVQRFTPGGDAGVVDSLT
                     KLSDKPLDLYKGRLAARAADDVYLVGAIEGEPDKTGLVARFDGKAWTAIAAPPGQEVT
                     WLALGADKAMWAIAGRSQPDAQATTALWKRVGEGPWTEAVLPAVRRPERAEPRWAFSV
                     SVDNWDEVPADLVEAARPMRVEAGQVHVRGDEVWVLARVPEADAHDYMRQVVLRSKPV
                     TRVLELPDPAYITAQTRTATAKPYDRKKGCESGPAWVPVATLAPGAAAAAAAPIAAAF
                     LAGLSPELQETFTTLREVEVRGGRVIALTFHPSEEHSTDELIAAVQRFRPDEQHKVEC
                     WNPRPTRSFYETEDPTSE"
BASE COUNT          552 a         1564 c         1278 g          582 t
ORIGIN      
        1 gcaggcgtcg tagatctggc cgcccgcgcc cgacgtgtcg agcgcgcaca ccaggccgtc
       61 ttcctgcggc gtgcagttgg cgccctcgag gcagttctgc tcgaaggcgt cgcacgccgg
      121 gaggcacagg tccagggcca cgccgaggcc gtgcgagcag aagctgccgt ccgggcagct
      181 cggcgaggcc gccgagccca tgcatagcgg cacgcacacg cccagctcgt cgccgtcgag
      241 gaagtagcag aagtcgccgc gcccgcagtc gtcgtcaccc gccccgaacc cgccggtcgc
      301 cgtgcacccg ccgccggtcg ggaccggcat cgccgggagc ggcacgcact tcgtcgcgtc
      361 gacgacggtg ttgtcgccca tcgtattgta atgcgtgcac ttctcgccct cggggcagtc
      421 ctgcagccac gggtcgcagg cgaccatcgg ctcctcgctc ggcgtgcctc cggtcggatc
      481 gtccggcggc ggatcggtgg cggatccgga cgatgcatcg gcgccggtgt ccgaggagcc
      541 gtcctcgccg gccccgtccc cggccggcgg ggtcgacgag cacgcgagca gcagcgagag
      601 cgcgagcgat ctgcggagca tcactcctgc ttatcgcgcg acgccccgcg cccgaaccgg
      661 gcccgcatcg gacccccgcg gacggtgctc gcggcgatga aacccactac gatgcccccg
      721 tgacgggccc cgcggtcgga actctcgtac gggactggcg cacgcgacgg cggcgcagcc
      781 agatggacct cgcgctcgac gtcggcgtct cgccgcggca cctcagcttc gtcgagaccg
      841 ggcgcgcacg cccgagcccc gagctgctgc tgtcgctcgc cgaacacctc gagctgccgc
      901 tgcgcgagcg caacgtcctg ctgctcgccg gcggctacgc gccgcgctac agcgagacct
      961 cgctcgacgc cgccgcgatg cagcgcgtgc gcgcggcgct gcagcgcatg ctcgacaccc
     1021 atcaccccta ccccggcgtc gtcatcgacc gctgctggaa catcatgctc gccaacgcgg
     1081 ccgccggcgc gctgctgcac ggcctgccgc cagcgctgct cggcccgccg accaacgtgt
     1141 ttcgcgtgtg cctgcacccc gagggcctgg cgccgcggac ccgcaacctg ccggagtggt
     1201 cggcctacct cctgggccag ctgcaccgcg cgaccctgct gaccggcgac ccggcgctcg
     1261 cgcggctgtc ggaggaggtc ggccgctacc cgaacctgca tgcgggtcca ccgcgccgcg
     1321 acagcgacga gccgaccctg ctcgtcccgg tgcgcctcga cgtcggcggc gccgagctgg
     1381 cgatgttcac gaccttgacg accttcggga ccccgcagga catcaccctc gccgagctcg
     1441 cggtcgagct gttcttcccg gccgacgccg cgagcgaggc gctgctgcgg ggcgcgcccg
     1501 gaccgcgcgc gtgatcgagc cattttttta ataatactac ctgcgaggta atcgcgggac
     1561 ccggggcggc gcggcacatt ggcgggcatg ccgactctca ccaccaccga tgatacacaa
     1621 ttatattatg acgactgggg caccgggcgc cccgtggtct tcgtgcacgg ctggagcctg
     1681 tgtaacgcca gctgggacca ccagctgccg gtgttcatcc gcgagggcct gcgctgcgtc
     1741 gcgcccgacc gccgcggcca cggtcgctcg agccgccccg gcactggcta cgacttcgac
     1801 accctcgccg acgacctcgc ggccctgctc gaccgcctcg acctgcgcga ggtcaccttc
     1861 gtcgcgcact cgatgggcgc cggcgaggtg gtccgttacc tgtcgcgtca cggcgccggc
     1921 cgggtcgcac ggatcgcact gctcgcgccg atcaccccgt atctgcgcaa atcgcccgac
     1981 aacccgcacg cggtcgacgc ggcgcagttc gagatgctcg ccgcggccct cggcgacgac
     2041 cgcgccgcgt ggttcgccgc cagcgcgccc ggcttcttcg gcgccggtca gccggactgc
     2101 cacgcctccg cggcgctgat cgactggggg atccgcatga tcctcgacac cccgctcaag
     2161 gtccagctcg acacattgca cacgcacacg agcaccgact ttcgccccga cctcgccgcc
     2221 gtgaatgtgc cgaccctggt gatccacggc gatatcgatc aatccgcgcc gatcgacctg
     2281 accgggcgcc cgaccgccgc gcagatcgca ggcgcccgcc tcgaggtcta cagcggcgcc
     2341 ccgcacggcc tgatgttcac ccaccagcag cgcctgcacc acgacctgct cgcattcatt
     2401 cgctagtcgg atcttccgtc tcgtaaaagc tgcgggtcgg ccgcgggttc cagcactcga
     2461 ccttgtgctg ctcgtccggc cgaaagcgct ggaccgcggc gatcagctcg tcggtgctgt
     2521 gctcctcgga cgggtgaaag gtcagcgcga tcaccctgcc cccgcggacc tcgacctcgc
     2581 gcaacgtggt gaacgtctcc tgcaactccg gcgagagacc ggcgaggaac gccgctgcga
     2641 tcggcgccgc cgcggccgcg gcggcccccg gagcgagcgt cgccaccggc acccacgcgg
     2701 gcccggattc gcagcccttc ttccggtcgt acggcttggc cgtggccgtc cgtgtctgcg
     2761 cggtgatgta cgccgggtcg ggcagctcga gcacccgcgt caccggcttc gagcgcaaca
     2821 cgacctgccg catgtaatcg tgggcgtccg cctcgggcac ccttgcgagc acccacacct
     2881 cgtcgccacg cacgtgcacc tggcccgcct cgacccgcat cggcctggcc gcctcgacca
     2941 gatcagcggg cacctcgtcc cagttgtcga ccgagacgct gaacgcccag cgcggctcgg
     3001 cgcgctctgg cctgcgcacc gccggcaaca cggcctccgt ccacggcccc tcgccgacgc
     3061 gcttccacag cgccgtcgtg gcctgcgcgt ccggctgcga gcgaccggcg atcgcccaca
     3121 tcgccttgtc ggcgcccagc gccagccacg tcacttcctg ccctggcggc gccgcgatcg
     3181 ctgtccatgc cttgccatcg aagcgcgcga ccagccccgt cttgtccggc tcgccctcga
     3241 tcgcgccgac gagatacacg tcatcggccg cgcgcgcggc cagcctgccc ttgtacaggt
     3301 ccagcggctt gtccgacagc ttcgtcagcg agtcgacgac ccccgcgtca ccgccgggcg
     3361 tgaaccgctg cacggagaag ctcgtcgtgc tctcgtcctc gccggtcgtc acgctcgacg
     3421 cgagcagcac gaacacctcg ccggtcggca gcgcggtgaa gtggatcggt gtggcaccgg
     3481 ccgcgagctt catcgtcgat ggcgtcatca cgccgcctgc cagcacgtcg aggcgcggcg
     3541 ggttggcgag ggtcgccgcg tcgatcttct tttgcagcga atccgggagc tccccatcat
     3601 acttttcata tagatcggtc ccgggcgcca tcatccgcag gccgagcacc tgcccgtccg
     3661 tgtacggcgc ataagcgccg tagaaccact cgagcagacc gacccgcgtc gccttgcgct
     3721 gccagcgttc gcccttgcgg tagtacatga gggggttgct cgacgagcgt tcgccgtacg
     3781 tctccaccga catccacacc gtgtccggcc agcgtccggc cagcatcagc aatcccagcc
     3841 cctccatgtt gctcggctcg atcaagcccc gctgcacgtc gggcatcagc tcgagcgcac
     3901 caccggcgcc gaggcgcgcc atcacgcccg cacccgcgac cacgagctcg ctgccgaggt
     3961 ccgcgagcgt gagatc
//
LOCUS       DASWNJ010001487         3957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_623069, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001487 DASWNJ010000000
VERSION     DASWNJ010001487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623069"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="VGB85_24725"
     CDS             complement(<1..844)
                     /locus_tag="VGB85_24725"
                     /inference="COORDINATES: protein motif:HMM:NF025670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HEY0137320.1"
                     /translation="MQLTFSQIKQFDFSWPAAAGATFYQLREQLTGTDDFIKIEDDIV
                     GESISLTMPLHLRFGAKYILSACNDGGCADSEPLEVMDSMAGAVGYFKASNTEGGDYF
                     GYSVALSSDGTTLAVGAQLEDSNATGVGGYQADNSSDKSGAVYVFVRSNGAWEHQAYV
                     KASNTGKGDQFGRSVALSADGVTLAVGASLEGSGAVYVFTRSASKWSQQTYLKASQAG
                     SLASFGTVAALSADGDTLVVGAHGEGSIAVGIDGDEGDDSAPAAGAAYVFVRSNESWT
                     QQAYI"
     gene            894..1175
                     /locus_tag="VGB85_24730"
     CDS             894..1175
                     /locus_tag="VGB85_24730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137321.1"
                     /translation="MVDVDSPVEGSLVWLAIVVPVSPIDASVSDALSVGSIPVGASVV
                     AVPVLAVVELDTSSEPSASIGSQPSPRLSHQLIIEIIAIQATLFMARPS"
     gene            complement(1199..2431)
                     /locus_tag="VGB85_24735"
     CDS             complement(1199..2431)
                     /locus_tag="VGB85_24735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137322.1"
                     /translation="MTPPFLFSALLSCGPVSPDPTYLIAPAADCHATIDARCSSLRGE
                     EALRCRNEADAQCDISDAERFIAEGDVSDTMSRLDQACAKYEDLLNMMLPDVDPAPIA
                     ETMIRYAGSSASIFALLAREGEVEDLTQAVQHLQRGRNFLINLLERRSELVGQPDLGT
                     ALEDLTKRLAAALDQLARQEMLRAAERYKEVRGAGTGDGGARSYYEAAVRHSAAAYTQ
                     RPTFAYRVVGFDAELAQAELDSELARSDRNAAAPACAGYRKLRHELADIERSAPRTWK
                     DNPQLHDLQRRAERGAGSCSARPRLIAGGVLVGVGTVSLGAALGLYAQYHAACDFAAA
                     QQSCTGISADGSDADRYTAQVQASIGLAVVGGALLTAGVTVLTHGLVQRKRARPRRFT
                     LAPALGPHQTGAVVSLRF"
     gene            complement(2428..3357)
                     /locus_tag="VGB85_24740"
     CDS             complement(2428..3357)
                     /locus_tag="VGB85_24740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137323.1"
                     /translation="MHTQLHEFFCAAFSAQQFRRWVRRLAPGGRGLAQALPGDDIDTL
                     TLFDRGIDLLIRHNYIDATFFVELAREFPKRHAELAELARKCGVPWTASGSSTLSASF
                     TSSIPHPRRGLPGAAIAAGACLAVGLIGYLRAASLAGPPPISTIRSIESDPVMPSSGV
                     WVPWQSAPATSTVLAKTTGPGGSHGGKRHGHAVEISAVVEQEKTGPASEPATTKPARV
                     ACALPGSLRAELQALAEVTLRRPGLSEEFTVLLMSTASIPQVSPSPGAGNATARKLYQ
                     GLVSLGPAQLGQCRDTKIDVVFSAYKTTVEPRI"
     gene            complement(3468..>3957)
                     /locus_tag="VGB85_24745"
     CDS             complement(3468..>3957)
                     /locus_tag="VGB85_24745"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEY0137324.1"
                     /translation="PLWQHARGVAAFVGVKGEPDTWGLLAATLAERKRLWLPRMEGQE
                     VAFVPVLDLRELAPAGFGLLEPRLRPGQVTLRAPLASAGIDLVLVPGLGFARDGGRIG
                     FGKGHYDRALAPVRDQAAPLRVGMCFADDLDPAEGPIPRGPQDVPMHWVVTESGLHRC
                     GA"
BASE COUNT          595 a         1321 c         1406 g          635 t
ORIGIN      
        1 tgatgtatgc ctgttgcgtc caggactcat tcgagcgcac gaacacatac gcggcgccag
       61 cggcgggggc actatcatct ccttcatccc cgtcgatgcc gacggcgatg ctcccttcac
      121 cgtgcgcgcc gacgacgagg gtgtcaccgt ccgccgacaa cgccgcgacg gtcccaaaag
      181 aggcgagtga accggcttgg gacgccttga gatacgtctg ctgtgaccac ttactggcag
      241 accgcgtgaa cacgtacacg gccccgctcc cttcgaggga ggcgccgacc gcgagggtaa
      301 cgccgtccgc cgacaaggcg acgctccgcc cgaattgatc gcccttcccg gtgttggacg
      361 cctttacgta ggcttgatgc tcccaggcac cgttcgagcg cacgaacacg tacaccgccc
      421 cgctcttgtc agacgagttg tcggcctgat atccaccgac cccggtggca ttgctgtcct
      481 ccaactgcgc gccgacggcg agggtggttc catccgacga cagcgcgacg ctgtatccga
      541 agtagtcgcc cccctcggtg ttggacgcct tgaaataccc gaccgcgccc gccatcgagt
      601 ccatgacttc cagcggctcg gagtccgcgc agccgccgtc gttgcaggcg ctcaggatgt
      661 atttcgcgcc gaagcgaagg tgcagcggca tcgtcagcga aatggactcg ccgacgatgt
      721 cgtcttcgat cttgataaaa tcgtcggtcc cggtcaactg ctcgcgcagc tgataaaacg
      781 tcgccccggc ggcggccggc cacgagaagt cgaactgctt gatctgcgag aaggtcagtt
      841 gcaactgcgt ggcctctggt ggggggccgc cggtcgagct cgcgtcgccc gtcgtggtcg
      901 acgtcgactc gccggtcgag ggatcgctgg tctggctcgc catcgtcgtg ccggtctccc
      961 ccatcgacgc ctcggtgagc gacgcgctgt cggtcgggtc catcccggtc ggcgcgtccg
     1021 tggtcgcggt cccggtgctc gcggtggtcg agctcgacac atcgtccgaa ccatcggcga
     1081 gcatcggctc acagccgtct cccaggctgt cacaccagct catcatcgag atcattgcga
     1141 tacaggcgac gctcttcatg gctcgtcctt cttgatgttc acgctcgcgg ctttctactt
     1201 agaagcgcag gctaacgacg gcgccggtct ggtgggggcc gagggccggg gcaagggtaa
     1261 agcggcgcgg cctggcccgc ttgcgctgga cgaggccgtg ggtcagcacc gtcacgcccg
     1321 ccgtcaatag ggccccgccg acgacggcga ggccgatcga ggcttgcacc tgcgcggtgt
     1381 atcggtcggc gtcggagccg tccgcggaga tccccgtgca cgactgctgc gccgccgcga
     1441 agtcgcaggc ggcgtggtac tgggcgtaga ggccgagcgc cgcgccgagg ctgaccgtgc
     1501 cgacgccgac cagcaccccg ccggcgatca ggcgcgggcg ggccgagcag gagccggcac
     1561 cacgctcggc gcggcgctgg agatcgtgca actgcgggtt gtccttccag gtccgcggcg
     1621 cgctgcgctc gatgtccgcg agctcatggc gcagcttccg gtagcccgca catgcggggg
     1681 ccgccgcgtt acggtcactt cgcgcgagct cgctgtccag ctccgcctgg gcgagctcgg
     1741 cgtcgaatcc gacgactcta tacgcgaagg tcgggcgctg cgtgtaggca gccgcgctgt
     1801 gtcggacagc cgcctcgtag tagctccggg cgccgccgtc tccggtcccg gcgccgcgga
     1861 cctccttgta tcgttcggcc gcgcggagca tctcctggcg ggcgagctgg tccagcgccg
     1921 cggcgaggcg cttggtgagg tcctcgagcg cggtgccgag gtcgggctgg ccgacgagct
     1981 cgctgcgtcg ctcgagcagg ttgatgagaa agttgcggcc ccgctgcaga tgctggacgg
     2041 cctgggtcag gtcctcgacc tcgccctcgc gggcgagcag cgcgaagatg ctggcgctgc
     2101 tgcccgcgta gcggatcatc gtctcggcga tcggcgcggg gtcgacgtcg gggagcatca
     2161 tgttgaggag atcctcgtac ttggcgcagg cttggtcgag gcggctcatc gtgtcactga
     2221 cgtcaccctc ggcgatgaat cgttccgcgt ccgagatgtc gcactgggcg tcggcctcgt
     2281 tgcggcaccg cagcgcctcc tcgccgcgca gcgagctgca gcgcgcgtcg atcgtcgcat
     2341 ggcagtcagc ggccggggcg atgagatatg tcgggtcggg cgagacggga ccgcacgata
     2401 acagggccga gaacaggaat ggtggcgtca tattctgggc tccacggtag ttttataggc
     2461 agagaacacg acgtcgatct tcgtatcccg gcattgaccg agctgcgcgg ggccgaggct
     2521 cacgaggccc tgatacagct tcctggccgt cgcgttccct gccccgggtg acgggctgac
     2581 ctgcggtatg gaggcagtgc tcatgagcag gacagtgaac tcctcgctga gccctgggcg
     2641 ccgcagggtc acctcggcca gcgcctgcag ttcggcccgc aggctccccg ggagcgcgca
     2701 ggcgacccgc gcgggctttg tggtcgccgg ctcgctggcg gggccggtct tttcctgctc
     2761 gacgaccgcc gagatctcga cggcatggcc gtggcgcttg ccgccgtgcg agccgcccgg
     2821 gccggtggtc tttgcgagga ctgtgctggt cgcgggggcg ctttgccagg gaacccagac
     2881 gccggagctc ggcatcacag ggtcggattc gatcgacctg atcgtcgaga tcgggggcgg
     2941 tccggcgagc gaagctgccc ggagatagcc gatcagcccg acagcgaggc atgcccccgc
     3001 tgcgatcgcc gcccctggca acccgcgccg cgggtgcggt atcgatgatg taaatgatgc
     3061 ggataatgtg gaggaaccag aggcggtcca cgggactccg catttgcgcg cgagctcggc
     3121 gagctcggcg tgtcgcttcg ggaactcgcg cgcgagctcg acgaagaatg tagcgtcgat
     3181 atagttatgg cggatcagca ggtcgatgcc cctgtcaaac agggtcaagg tgtcgatgtc
     3241 atctccgggt agcgcctgcg cgaggccgcg gccgcccggc gcgagccgtc gtacccagcg
     3301 gcgaaactgc tgcgcagaga atgccgcgca gaagaattca tggagctggg tatgcatcga
     3361 cgtgctgact agtaccggcg gccagcaccg acgatcacaa aaatcgtact cggcgtgccc
     3421 gcaagacttg ctgccgtcga ccggacgccg ccccgcgggc gcggggctca ggcgccgcag
     3481 cggtgcaggc cgctctcggt gacgacccag tgcatcggga cgtcctgcgg cccgcgtggg
     3541 atcgggccct cggcggggtc gaggtcgtcg gcgaagcaca tgccgacgcg cagcggcgcg
     3601 gcctggtcgc ggaccggcgc gagcgcgcgg tcatagtggc ctttaccaaa gccgatccgg
     3661 ccgccgtcgc gggcgaagcc gaggccgggc acgagcacga ggtcgatgcc ggccgaggcc
     3721 agcggggcgc gcagggtgac ctggcccggg cgcaggcgcg gctcgagcaa gccgaagccg
     3781 gcgggcgcga gctcgcggag gtcgagcacc ggcacgaacg cgacctcctg gccctccatg
     3841 cgcgggagcc acaggcgctt gcgttcggcg agggtcgccg cgagcagacc ccaggtgtcg
     3901 ggctcgccct tgacgccgac gaaggccgcg acgccgcgcg cgtgttgcca caacggg
//
LOCUS       DASWNJ010001488         3656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_557979, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001488 DASWNJ010000000
VERSION     DASWNJ010001488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557979"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..1818)
                     /locus_tag="VGB85_24750"
     CDS             complement(160..1818)
                     /locus_tag="VGB85_24750"
                     /inference="COORDINATES: protein motif:HMM:NF005511.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY0137325.1"
                     /translation="MTSESTEHEPLRGDDRGVRDGGRTHAWDTTCDVVVVGLGAAGGS
                     AAIEARSLGAEVLVVDRFGGGGATGKSAGIIYFGGGTDLQKSAGYEDTPENMFNYLAL
                     EIGDAVTPETLRRFCETSVANFEWLRRLGVPFPASGSACKSSYPSDDCTLYFSGNELC
                     HPYADHATPAPRGHRPLGRGLTGHLSAEAMRHGALELGVTLRTFTRAERVVVDRDGDV
                     IGVVLCELSSPRLRHPASEAMELLVRYGGGISSRIGGVLQGTLRALERGCVRRYVRAR
                     KGVVLGTGGFVFDKELMQEHSPKYAGCSMRLGTAGDDGSGIKIGKAAGGALGKMDRCT
                     AWRFIDPPNAWWGGILVGRNGERICNETLYGGKVGEHLIEHHGGQGTLILDAGMMARG
                     RKQVMEGKPQLYQRAFGLINGYLTCKSAPTLRKLAARLGIDADRLEQTVAAYNTGVTN
                     GVDALGKHGDYLGAITEGPFYGIPLDHDSLLFPTPCLTLGGLRTDGASGRVLRDDGAA
                     IEGLYAAGRTAIGVSSNGYVSGLSVADGIFSGRTAGRHAVARTT"
     gene            1865..2656
                     /locus_tag="VGB85_24755"
     CDS             1865..2656
                     /locus_tag="VGB85_24755"
                     /inference="COORDINATES: protein motif:HMM:NF014590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY0137326.1"
                     /translation="MRWYTGDPVYDTILLVALLMPIFTVLALRFLNAPYGRFGDTWPW
                     AKLDARLGWMLMELPATLVFWPAFLTGPRAAATAPMVIAGIWGLHYLNRGFLFPLMMR
                     VPRGSRTFSVFLVAFGMIVTSIHGYLSGYYISRYADHLTADWLCDPRFLVGVALYAGG
                     LALNIHSDAVVRRLRSAAEVERGTKVYRIPRGGGYHVVSNPQYLGELIAWTGFALLTW
                     SLAGVFILTLSAANLIPRAIANHRWYHRTFPDYPRHRRILVPFVF"
     gene            2680..>3656
                     /locus_tag="VGB85_24760"
     CDS             2680..>3656
                     /locus_tag="VGB85_24760"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0137327.1"
                     /translation="MTGATGQVGLELCRALRAADKVVRAVVLPGDRQAQLLRDLGIEV
                     VTADVRDLAALQLAFIGADTVYHLAAIVSTSAKHDPRMWQVNVEGARNAAVATKAAGA
                     RRMVYFSSIVVFDPAPHDDPLDETRPRLEVTVGSPYVRSKVVAEAIVRSAVAGGLDAV
                     IVHPTVVIGANETHHIGVVRSLFFAYFGGTLPATVRGGFNAVAVGDVVAGAIAAAERG
                     RTGESYILGGEYGSLTDMLRRVQPLSGARLPRLSVPMVMARAGLPVMAAFARITRTRP
                     TFTPEDLRQLDGNRQISCQKAARELGYRPHGLDVALRQVHDAWLRRDLAG"
BASE COUNT          476 a         1277 c         1304 g          599 t
ORIGIN      
        1 cctcgaccgg gtcctggacg aagccgtccg cgtggaacag gccgacccag ccgtgccggt
       61 ccttggcgcc gacgagcgcg ggtgacttga gggggagggc gaaggtgctg cgggggtcca
      121 tcgtggtgct tcgatcgtga ggggtcgagt gagtgtcgct caggtcgtgc gtgcgacggc
      181 gtggcggccg gcggtgcgcc cggagaagat gccgtcggcg accgagagcc cgctgacgta
      241 gccgttggac gacacgccga tcgcggtgcg gccggcggcg tagaggcctt cgatcgccgc
      301 gccgtcgtca cgcagcacgc ggccgctggc gccgtcggtg cgcaggccgc cgagcgtcag
      361 gcacggggtc gggaacagca gcgagtcgtg gtcgagcggg atgccgtaga acgggccctc
      421 ggtaattgcg ccgaggtagt cgccgtgctt gccgagcgcg tcgacgccgt tggtgacgcc
      481 cgtgttgtag gcggcgacgg tctgctcgag gcggtcggcg tcgatgccga ggcgcgccgc
      541 cagcttgcgc agggtcggcg cggacttgca ggtcaggtag ccgttgatca ggccgaacgc
      601 gcgctggtac agctgcggtt tgccctccat cacctgcttg cggccgcggg ccatcatgcc
      661 ggcgtcgagg atcagcgtgc cctggccgcc gtggtgctcg atcaggtgct cgccgacctt
      721 gccgccgtag agggtctcgt tgcagatgcg ctcgccgttg cggccgacga ggatgccgcc
      781 ccaccacgcg ttcggcgggt cgatgaagcg ccacgcggtg cagcggtcca tcttgccgag
      841 cgcgccgccg gccgccttgc cgatcttgat ccccgagccg tcgtcgccgg cggtgccgag
      901 gcgcatgctg cagccggcgt acttcggcga gtgctcctgc atcagctcct tgtcgaacac
      961 gaagccgccg gtcccgagca cgacgccctt gcgcgctcgc acgtagcgcc gcacgcagcc
     1021 gcgctcgagc gcgcgcaggg tgccctgcaa cacgccgccg atccgcgagc tgatgccgcc
     1081 gccgtagcgg accagcagct ccatcgcctc gctcgccggg tgacgcaggc gcggcgagct
     1141 cagctcgcac agcacgacgc cgatgacgtc gccgtcgcgg tcgacgacga cgcgctcggc
     1201 gcgggtgaag gtccgcagcg tgacgccgag ctcgagcgcg ccgtggcgca tcgcctcggc
     1261 cgagaggtgg ccggtcaggc cgcggccgag cggacggtgc ccacgcggcg ccggcgtggc
     1321 gtggtcggcg taggggtggc acagctcgtt gcccgagaag tacagcgtgc agtcgtcgga
     1381 cgggtacgac gacttgcagg cggagccgct ggcggggaag ggcacgccga ggcggcgcag
     1441 ccactcgaag ttggcgacgc tggtctcgca gaagcggcga agggtctcgg gcgtgacggc
     1501 gtcgccgatc tcgagcgcga ggtagttgaa catgttctcg ggcgtgtcct cgtacccggc
     1561 ggacttttgc aggtcggtgc cgccgccgaa gtagatgatg cccgcgctct ttccggtcgc
     1621 cccaccgccg ccgaagcggt cgacgacgag cacctcggcg ccgagggatc gggcctcgat
     1681 cgccgcgctc ccgccggccg cgccgaggcc gacgacgacg acgtcgcagg tggtgtccca
     1741 ggcatgcgtg cgcccgccgt cgcggacgcc gcgatcatcg cctcgcagcg gctcatgctc
     1801 ggtcgactcg ctggtcatcg ccgctggttt actattttct cgcgggtcgt gctacacgct
     1861 gcaaatgcgc tggtacacgg gcgatccggt ctacgacacg atcttgctgg tggcgttgct
     1921 gatgccgatc ttcacggtgc tggcgctgcg cttcctcaac gcgccttacg ggcgctttgg
     1981 cgacacgtgg ccgtgggcca agctcgacgc gcggctcggc tggatgttga tggagctgcc
     2041 ggcgacgctc gtgttctggc cggcgttcct caccggaccc cgcgcggccg ccaccgcgcc
     2101 gatggtgatc gcggggatct ggggtctgca ttacctcaac cgtggcttct tgtttccgct
     2161 gatgatgcgc gtcccgcggg gcagccggac cttcagcgtg ttccttgtcg ccttcggcat
     2221 gatcgtgacg tcgatccacg gttatctcag cggctattac atttcgcgct acgcagatca
     2281 cctcaccgcg gactggctct gcgacccccg ctttctcgtc ggtgtcgcat tgtacgcggg
     2341 cggactcgcg ctcaatatcc actccgatgc ggtggttcgt cgcctgcgca gcgcagccga
     2401 ggtcgagcgc ggcaccaagg tctatcgcat ccctcgcggc ggcggctatc acgtggtcag
     2461 taatccgcag tacctcggcg agctgatcgc gtggacgggc ttcgcgctgc tgacctggtc
     2521 gctcgcgggg gtcttcattt tgacactgtc ggccgcgaac ctcatcccgc gcgcgatcgc
     2581 caatcatcgc tggtaccacc gtaccttccc tgactatccc cggcatcgcc ggatcctggt
     2641 accgttcgtg ttctgacgaa cacaagtggg atccaggtgg tgaccggcgc gaccggtcag
     2701 gtcggcctcg agctgtgtcg agcgctgcgc gccgcggaca aggtggtacg cgccgtcgtg
     2761 ctgccgggcg accggcaagc ccagctgctg cgcgacctcg ggatcgaggt cgtcaccgcg
     2821 gatgtccgcg acctcgcggc gttgcagctc gcgttcatcg gcgccgacac ggtgtaccac
     2881 ctcgccgcga tcgtcagcac gagcgcgaag catgacccgc gcatgtggca ggtcaacgtc
     2941 gagggcgccc gcaacgcggc cgtcgcgacg aaggccgccg gcgcgcggcg catggtctac
     3001 ttctcgtcga tcgtggtgtt cgacccggcc ccgcacgacg acccgctcga cgagacgcgc
     3061 ccgcgcctgg aagtgaccgt cggatcgccc tacgtgcgct cgaaggtggt cgccgaggcg
     3121 atcgtccgct cggcggtcgc cggcggcctc gatgcggtca tcgtccaccc gacggtcgtc
     3181 atcggcgcca acgagacgca ccacatcggc gtcgtccgca gcctgttctt cgcgtacttc
     3241 ggcggcaccc tgccggccac cgtgcgcggc ggcttcaatg cggtcgcggt cggcgacgtc
     3301 gtcgcgggcg cgatcgctgc cgccgagcgc gggcgcaccg gcgagagcta catcctcggc
     3361 ggcgagtacg gctcgctgac ggacatgctc cgccgcgtgc agcccctcag cggcgcccgc
     3421 ctgccgcgcc tgtcggtgcc gatggtgatg gcgcgcgccg gcctcccggt gatggcagcg
     3481 ttcgcccgca tcacccgcac gcgcccgacc ttcacccccg aggacctgcg ccagctcgac
     3541 ggcaaccgcc agatctcgtg ccagaaggcg gcgcgcgagc tcggctaccg accccacggt
     3601 ctcgatgtcg cgctgcgcca ggtgcatgac gcgtggctgc gtcgtgacct cgccgg
//
LOCUS       DASWNJ010001489         4114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_27044, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001489 DASWNJ010000000
VERSION     DASWNJ010001489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27044"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VGB85_24765"
     CDS             <1..528
                     /locus_tag="VGB85_24765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137328.1"
                     /translation="DATGQHVWSQQFGPEGLQYGSRIAVDGAGDVTLGGKYLEGITIG
                     PDSYVNTWPNANEEVDDTLYDGFLIHLDGAGALQSSMQIGTKLDDAVIDLKFDSDGVL
                     LMTAFTDESVVVQAFVDGKPGWKWSTSALQWSSTAVTTNAVVLGASIGVEIDLGGGVL
                     AARGDEDMIIAKILR"
     gene            714..1442
                     /locus_tag="VGB85_24770"
     CDS             714..1442
                     /locus_tag="VGB85_24770"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HEY0137329.1"
                     /translation="MTHGERRPQILAMGGGGFSMEPDNLALDRYLLRLTGEPRPRVCF
                     VPTASGDAEGYIAGFYTAFNSLDCEPSHLGLFRRNHADLRAHVLSQHAIYVGGGNTRN
                     MLALWREYGLDHVFREAWRAGVVLAGLSAGSLCWFESGVTDSNGALAAMACLGFLPGS
                     HCPHYDSEPERRPAYHGLLASDAIAAGYAADDGAALHFIDTTLAAVVSSRPDARAYRV
                     AQGPAGASETPLLAELLAAPAPVA"
     gene            1725..3989
                     /locus_tag="VGB85_24775"
     CDS             1725..3989
                     /locus_tag="VGB85_24775"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY0137330.1"
                     /translation="MTDHSDDSPPHGQLLVAKGWVQASAMVVLFGFFVLGLLAYRTYI
                     AGPPIPAQVVTDDEVAVFSGADVQEGQRIFLRTGLMQYGSIFGHGAYLGPDFTADYLR
                     RAATHVRDGYERAGARSPGERVADDFKNNRYDPATGVLRFNAPQADAFVAVREHYTRF
                     FAAPSSETGLTLQGQLAPADLHALTSFFAWSAWVASAQRPGKDYSYTNNWPPEPLVRN
                     HPTAAALVYSMLSLAALLGGAGLLFAAFGRWKFLGWHGEEERRLLWRRPDEVRLSPAQ
                     RACAWFFFVMAALFLVQVLVGAASQHYRADLATFFGVDLARTWHVQLSIFWVATSFLA
                     AGIFIAPLVAGHEPRRQHLLAYGLLAALTIVVVGSLGGELAGIHGWTTNIWFGNQGFE
                     YLDLGRFWQLLLSLGLTAWAAILFRGLRGRLRGEHPGNMPWLFFYGALAIPAFYAVGL
                     LAHPDMNFTTADFWRFWVVHLWVEDFLELFTTIMVAYIFVLLGVVRERVALRLIYLDI
                     ILYSAGGVIGSMHHVYFSGEPVEHMALGAIFSAAEVMPLTFLTLESWTFLRLGRRPGA
                     DETHPFPHRWAIMFLVAVGFWNFLGAGIFGFLINLPIVSYYEIGTALTANHAHASMMG
                     VYGMLAVGLALFGLRYLIPEDRWSDRAARVSFWSLNLGLAWMVFVSLFPLGILQLFES
                     VNHGYYEARTLAFLTNPTNQLLEWLRLPGDFVFIVGGVLPLLYLCWLGVWHRGRPTMN
                     PDTRAGTLFTATEP"
BASE COUNT          545 a         1483 c         1346 g          740 t
ORIGIN      
        1 gacgcgaccg ggcagcacgt gtggagccag cagttcgggc cggaggggct gcaatacggc
       61 agcaggatcg cggtcgacgg ggccggcgac gtgacgctcg gcggcaagta cctcgagggc
      121 atcacgatcg gccccgacag ctatgtcaac acctggccga acgccaacga ggaggtcgac
      181 gacaccctct acgacggctt cctcatccac ctcgacggag ccggcgcgtt gcagtcctcg
      241 atgcagatcg gcaccaagct cgacgacgcc gtgatcgacc tgaagttcga cagcgacggc
      301 gtgctgctga tgaccgcctt caccgacgag tcggtcgtcg tgcaggcgtt cgtcgacggc
      361 aagccgggct ggaagtggtc gacgtccgcc ctgcagtggt cgtccaccgc ggtgaccacg
      421 aacgcggtcg tgctcggcgc gtccatcggc gtggagatcg atctcggtgg tggtgtgctc
      481 gccgcgcgtg gcgacgagga catgatcatc gccaagatcc tccggtgagg cccggccccg
      541 ccggctacgc gcgcttgcga tggcgcagca cggccttcgc cagctcaaca gccgcagcgg
      601 cgtggagcgc gtcaacgccg tgtgccccgg gattatcgac ccaccgatga tgcagcgctt
      661 gaccggcagc acctccgaac gtgtagcgct gcgatggatg gtatgctcgg ccgatgacgc
      721 acggcgaacg tcgaccccag atccttgcga tgggaggcgg tggcttctcg atggagcccg
      781 acaacctcgc gctggaccgc tacctgctgc gcctgaccgg cgaaccacgc ccgcgtgtct
      841 gcttcgtgcc gaccgccagc ggcgacgccg agggctacat cgcggggttt tataccgcgt
      901 tcaattccct cgactgtgag ccgagccacc tcgggctgtt tcgtcgcaac cacgccgacc
      961 tgcgcgcgca cgtgctcagc cagcacgcga tctacgtcgg cggcggcaac acccgcaata
     1021 tgctcgcact ttggcgcgag tacgggctcg accacgtgtt tcgcgaggcc tggcgcgccg
     1081 gcgtcgtcct ggccgggctg tcggccggct cgctgtgctg gttcgagtcc ggcgtgaccg
     1141 actcgaacgg cgcgctcgct gcgatggcct gcctcgggtt tttgccgggc tcgcactgcc
     1201 cccattacga cagcgagccc gagcggcgtc cggcctatca tggcctgctg gcgagcgacg
     1261 ccatcgccgc gggttatgcc gccgacgacg gcgccgcgct gcacttcatc gacaccacgc
     1321 tcgcggccgt ggtctcgtcg cggcccgacg cccgcgccta tcgcgtcgcc cagggccccg
     1381 cgggtgccag cgagacaccg ctgctcgcgg agctgctcgc tgcccccgcc ccggtcgcgt
     1441 gaggcggcag tggcggtcgg cgtcgccgga tctccgcgtt cgatgcgcag tcgaggagct
     1501 cggcatttgc gcggatgtcg cgtaatcgcg gtgccagcgg acggcccgtg gctgggcgcg
     1561 cgcgtatttg cgaggaatac tgtaatctcg ttcgcgcttg ccatggtttt ccccacggtg
     1621 cggccatcag ccacttccag gcgctgtcgg ccgcgctctc ggcgactgtc gggctcggca
     1681 acatcgcatg cgtgtattcc accgacaaga cagcgcgagg cccgatgaca gatcacagtg
     1741 acgactcccc gccgcacggg caactcctgg tagcgaaggg ctgggtgcag gcctcggcga
     1801 tggtcgtgct gttcggcttc ttcgtgctcg ggctgctggc ctatcgcaca tacatcgccg
     1861 ggccgccgat ccccgcgcag gtcgtgaccg acgacgaggt cgcggtgttc tccggcgcgg
     1921 acgtgcagga gggccagcgc atcttccttc gcaccgggct catgcaatat gggtcgatct
     1981 tcgggcacgg cgcctatctc ggccccgact tcaccgccga ttacctccgg cgcgccgcga
     2041 cccacgtccg cgacggctac gagcgcgccg gagcgcgcag cccgggcgag cgtgtcgccg
     2101 acgattttaa aaataaccgc tacgaccccg cgaccggggt gctacgcttc aacgcgcccc
     2161 aggcggacgc cttcgtcgcg gtccgcgagc attatacgcg cttcttcgcc gcgccctcgt
     2221 ccgagaccgg gctgacgctc cagggccagc tcgccccggc cgacctccac gccctcacga
     2281 gcttcttcgc ctggtcggcc tgggtcgcct ccgcgcagcg acccggcaag gactactcct
     2341 ataccaacaa ctggccgccc gagccgctgg tacgtaatca cccgaccgcg gccgccctcg
     2401 tctacagcat gctgtcgctc gccgccctgc tcggcggggc cggcctgctg ttcgccgcgt
     2461 tcgggcgctg gaagttcctc ggctggcacg gtgaggagga gcgccgcttg ctctggcgcc
     2521 ggccggacga ggtccgcctg tcgccggccc agcgcgcctg cgcgtggttc ttcttcgtca
     2581 tggccgcgct gttcctcgtc caggtgctcg tcggcgccgc ctcgcagcac tatcgggccg
     2641 acctagcgac cttcttcggc gtcgacctcg cccgcacctg gcacgtgcag ctctcgatct
     2701 tctgggtcgc gacctctttc ctggccgcgg ggatcttcat cgcgccgctc gtcgccggcc
     2761 acgagccgcg ccgccagcac ctgctcgcgt acggcctgct cgcggccctg acgatcgtcg
     2821 tcgtcggcag cctcgggggc gagctcgcgg gcatccacgg gtggaccacc aacatttggt
     2881 tcggtaacca gggcttcgag tacctcgacc tcggccgctt ctggcagctg ctgctctcgc
     2941 tcggcctgac ggcgtgggcg gcgatcctgt ttcgcggcct gcgcgggcgc ctgcgcggcg
     3001 aacaccccgg caacatgccc tggctgttct tttatggcgc gctggcgatc cccgccttct
     3061 acgcggtcgg gttactcgcg caccccgaca tgaacttcac caccgccgac ttctggcggt
     3121 tctgggtcgt gcacctgtgg gtcgaggact tcctcgagct gttcacgacg atcatggtcg
     3181 cgtacatctt cgtgctgctc ggcgtcgtcc gcgagcgcgt cgcgctgcgc ctgatctacc
     3241 tcgatatcat cctctactcg gccggcggcg tgatcggctc gatgcatcac gtgtatttct
     3301 ccggcgagcc ggtcgagcac atggcgctcg gggcgatctt ctccgccgcc gaggtcatgc
     3361 ccctgacgtt tctgacgctc gagtcctgga cctttctgcg cctgggccgg cgcccgggcg
     3421 cggacgagac ccacccgttc ccgcatcgct gggcgatcat gttcctggtc gcggtcggct
     3481 tctggaactt cctcggcgcc ggcatcttcg ggtttttgat caatctgccg atcgtctcgt
     3541 attacgagat cgggaccgcc ctgacggcga accacgccca cgcctcgatg atgggggtct
     3601 acggcatgct cgccgtcggg ctcgcgctgt tcgggctgcg ctacctgatc ccggaggacc
     3661 gatggtccga tcgcgccgcc cgcgtcagct tctggtcgct caacctcggc ctcgcctgga
     3721 tggtcttcgt gtcgctgttt ccgctcggga tcttgcagct cttcgagtcc gtgaaccacg
     3781 gctattacga ggcccgcacg ctcgccttcc tgaccaaccc gaccaaccag ctgctcgagt
     3841 ggctgcggct ccccggcgac ttcgtcttca tcgtcggcgg cgtcctgccg ctgctgtatc
     3901 tgtgctggct cggcgtgtgg catcgcgggc gacccaccat gaaccccgac acgcgcgcag
     3961 gcaccctgtt caccgccacg gagccgtaga gagccttgaa gaattactgg catcgcggcc
     4021 gcccggcccg cgcgcgtgag gggagggcgg cgggtcaggc cgccgtggcc gccggcgcgt
     4081 cgatcaggat gcgctggcgc ggcagcgcga tatc
//
LOCUS       DASWNJ010001490         2623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_592183, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001490 DASWNJ010000000
VERSION     DASWNJ010001490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592183"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1009)
                     /locus_tag="VGB85_24780"
     CDS             complement(41..1009)
                     /locus_tag="VGB85_24780"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY0137331.1"
                     /translation="MIESLPVLTVRYPALLPETTAFVAVAGPADAALARVARGDPQFA
                     LGVFVRQDGVASGLTGLMRIGCVAVVEEVVVERGQLLGLRLRGLARAELLGPFEEADA
                     ALLRARVRVLDAPAAVDPLLRAQLARVRTRLPNAGLTLDAPELAALMAVEEPGHFADL
                     LAAQLDDLSLEARLALLTTLAPAARLVLVDRLLDARAPAPTSELGRVWARLREPCDAV
                     PGHASLRARAMQIDVAALHDLRLRHMVEELARARPILVVEVDDSRELEARRESLSQLT
                     SAMRSLTALRECGGPDDAALQAEIAASVAFLLAVASRERAEIDRAR"
     gene            complement(1185..1619)
                     /locus_tag="VGB85_24785"
     CDS             complement(1185..1619)
                     /locus_tag="VGB85_24785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137332.1"
                     /translation="MRCIYCKTDSLVQLDAGHTRALAGKAVQAAANAEHALSMLRLRA
                     SSFDRNRTGCAAFAVLLLLIVVLFTFVSQGWAILLILAMAGAAVLAFLLMADFHVVRG
                     QGLTLEDGISGEAARAMGMGWVVFAIAMTLIMMIMTGVLVME"
     gene            1609..1977
                     /locus_tag="VGB85_24790"
     CDS             1609..1977
                     /locus_tag="VGB85_24790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137333.1"
                     /translation="MQRTNNASGPGAPSGAPQERHPNVNPGRAANAAVTLAMGPAGTL
                     CQAEYDAQYSAGPSGSQNSPRTVVCARPGSKTPALTSTLAVTPVTINTGARWEAMAAF
                     QTGIVCSTSERHSCEARASS"
     gene            2293..>2623
                     /locus_tag="VGB85_24795"
     CDS             2293..>2623
                     /locus_tag="VGB85_24795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137334.1"
                     /translation="MKYRPLSLVLVSLVACGDGGSATTTDASTTMLTSTTATSTGDTP
                     TTSTSTTSDPQTTTGASQGESEVSTSPSPTTSTSATDSTGEALTSTSTGPDTTTGDTT
                     ESDTSTGE"
BASE COUNT          432 a          989 c          914 g          288 t
ORIGIN      
        1 cgctcgagtc gcaggtcgtc gccgcgcgcc tgccctgagg tcagcgggcg cggtcgatct
       61 cggcgcgctc gcggctcgcg accgcgagca ggaaggcgac cgacgcggcg atctcggcct
      121 gcagcgcggc gtcgtcggga ccgccgcact cgcgcagcgc cgtcagcgag cgcatcgccg
      181 aggtcagctg cgacaggctc tcgcgccgcg cctcgagctc gcggctgtcg tcgacctcga
      241 cgacgaggat cggtcgggcc cgcgcgagct cctcgaccat gtgccgcagg cgcaggtcgt
      301 gcaacgccgc gacgtcgatc tgcatggccc gcgcgcgcag gctcgcgtgc ccgggcaccg
      361 cgtcgcatgg ctcgcgcagc cgggcccaca cgcgaccgag ctcgctcgtc ggcgccggcg
      421 cgcgggcgtc gagcaggcgg tcgacgagca ccaggcgggc cgccggcgcg agcgtcgtca
      481 acagggccag gcgcgcctcg aggctcaggt catcgagctg cgcggcgagc aggtcggcga
      541 agtgacccgg ctcctcgacc gccatcagcg cggcgagctc cggagcgtcg agcgtcaggc
      601 cggcgttcgg caggcgcgtc ctgacccgcg ccagctgggc gcgcagcagc gggtcgaccg
      661 ctgcgggtgc atcgagcacc cgcacgcgcg cgcgcagcag cgccgcgtcg gcctcctcga
      721 acggcccgag cagctcggcg cgggccagcc cacgcaggcg cagcccgagc agctggccgc
      781 gctcgacgac cacctcctcg acgaccgcga cgcagccgat ccgcatcagc ccggtgagcc
      841 cggacgcgac cccgtcctga cgcacgaaca cgccgagcgc gaattgcggg tcgccccggg
      901 ccacccgcgc gagcgcggcg tcggcgggac cggcgaccgc gacgaaggcg gtggtctcgg
      961 gcaacagcgc cgggtagcgc acggtcagga cagggagcga ctcgatcacc ggccgggcgt
     1021 agcacgaacc cgcaaaggcg cacgaccatg gcggccggcg cgacacacga gcgccgtgcc
     1081 gtgaccccga cgctcccggc gcgctgtcgc cgaggacctc gaggacctgg gagcgctggt
     1141 gcggcgtgcg gcgcggtcgt cgcacgacct tcacagcggc acggtcactc catcacgagc
     1201 acgccggtca tgatcatcat gatgagcgtc atcgcgatgg cgaagaccac ccagcccatg
     1261 cccatcgccc tggcagcctc gccgctgatg ccgtcctcga gggtcaatcc ttgtcctcgc
     1321 acgacatgga aatcggccat caacaggaac gccagcaccg ccgccccggc catggcgagg
     1381 atcagcagta tggcccagcc ctgcgacacg aacgtgaaca gcacgacgat gaggagcagg
     1441 agcacggcga aggcggcaca tccggtgcga tttctgtcga agctcgaggc acgaaggcgg
     1501 agcatcgaga gcgcatgctc ggcgttcgcg gcagcctgca cggccttgcc cgcgagcgcc
     1561 cgggtgtggc ccgcgtcgag ctggacgagg ctgtcggtct tgcaatagat gcagcgcacg
     1621 aacaacgcct cgggtcccgg ggcgccgagc ggggcgccac aggagcggca cccgaacgtc
     1681 aacccgggtc gagcagcgaa cgccgccgtg accctggcca tgggtcccgc cgggacgctg
     1741 tgccaggccg aatacgacgc ccagtacagc gccggcccga gcggcagcca gaacagcccg
     1801 aggaccgtcg tgtgtgcgag gccggggtcg aagacgccgg ccttgaccag cacgctggcc
     1861 gtcacgcccg tgaccatcaa caccggcgcc aggtgggagg cgatggccgc cttccagacc
     1921 gggatcgtgt gctcgaccag cgagcgccac agctgtgagg cccgcgccag ctcctgatcg
     1981 gcggcgcgcg tgaggcgtac tgcctgtcga tgactggcag gcatcaccct cgcggtgcca
     2041 cacgccaggc actggacgtg gtcggtatcc gcggccggca ccggcgcacc acactgctcg
     2101 caggagagag gagtcggagt cgcctcggtc atgctggtcg acccatctta gcgcatccgc
     2161 gcgagccgat cggcctcgcg ttgcggccga tcatgcatcc gcgcgccggc cccgtggccg
     2221 cgccgcgagt cggatacacc cactccacga gcctggctcc ctgcaatgac acacggtacg
     2281 gtgtccgact ccatgaaata ccgtcccctg tcgctcgtcc tcgtgtcgct cgtggcctgt
     2341 ggtgatggtg gctccgcgac gaccaccgac gcgagcacca ccatgctcac gagcaccacc
     2401 gccacgtcga ccggcgacac cccgaccacc agcaccagca ccaccagcga cccgcagacg
     2461 accacgggcg cctcgcaggg cgagtccgag gtcagcacga gcccgtcgcc gaccacgagc
     2521 acgagcgcga ccgacagcac cggcgaggcg ctgacgagca cgagcaccgg ccccgacacg
     2581 accaccggcg acacgaccga gagcgacacc agcaccggcg agc
//
LOCUS       DASWNJ010001491         4160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_558042, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001491 DASWNJ010000000
VERSION     DASWNJ010001491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558042"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="VGB85_24800"
     CDS             <1..976
                     /locus_tag="VGB85_24800"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0137335.1"
                     /translation="FAALTPEDAAGPGRVLAEVGPELRTIAETATDMHLRANASLLLG
                     TLYEATGDPRSAISFYRQVVALLPNDPEPHRVLAMALAADKQFAAALPEQELVVKDDP
                     DDLEAWLLLGELAVKAGEKDQATQAYGAYELRRKGLIDGISLKKADGTFPLPAEQRAA
                     CARALIPARDNGTAIALLYALEIETDPGVRVAIVEAMGAQRLAGYKPTLETKLKTETA
                     QEVKEAMIWALAEIQRDPLDTRPGPTPQGPGTGEDAKAGDAAGEAAGDPKAGDVKAES
                     AKAEDAKATDVKAATKVDDAKATGAKGQVPADRSAGTDAKAGTPAPSR"
     gene            979..1995
                     /locus_tag="VGB85_24805"
     CDS             979..1995
                     /locus_tag="VGB85_24805"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0137336.1"
                     /translation="MRPPASLEIAALSHRYPGAAVPALAQVGFRVAPGERVGLLGPNG
                     AGKSTLMRLLCGYLPVQRVPGTRVRVGEVEVAADSLRARQLVGYLPEQVPLYPELRVR
                     EHLDFRASIKGVRRKARPPEILRVAERTGLSHMLEVPIQQLSRGYRQRVGIADALLGA
                     PPLVVLDEPTVGLDPNQVLEIRAMLRALGGTQTLIFSSHILAEVEALCDRVVILAGGK
                     CVADEAVAEALRGSDVIVAWMIAQADAEIVLASALRDMSAGTGDLTTLADGLGLRLGS
                     FEARGDAAQLRITCPDAQVAEALARAIGRASAAAGVALARLELGRGRLEERFARVTGA
                     VAGA"
     gene            1992..>4160
                     /locus_tag="VGB85_24810"
     CDS             1992..>4160
                     /locus_tag="VGB85_24810"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEY0137337.1"
                     /translation="MSTWDTPVEPLQRPGPWLRGAYHVARRELLSLFVTPLAYLVGTL
                     FLLNQGWNFALLLRVLNDPLAAPGPVMQFYFGGSFFIFWLPVIFICAALSMRLIAEER
                     RQGTLEALLTAPLSPSQIALGKFAGAYGFYLALWLPTATFYLLLLGAGARPEAGPVLA
                     GYLGVLLVGASFLSVGLLASALARSQLAAAISTFVTCTIAVMAGLLSEQVQAGTTAQL
                     LHFTSLLVMMQELAQGIVDPRWVYLHLGFTATLLAATIVALNPRRRREHAVQVVLLAL
                     LLGHLTVFGARHGTRDDWTAGHVYTLSDRAREVLGGLKTPIDVSVIVPATIGGGRPNP
                     LLAELREVLARMGAAAPNLRIRWVDPDRDHAQAQASIAEFALGGRELADGVVLLRAGQ
                     GASLRKAHLLPGELVTFATGPDVQQTGPRVREFRGEEALLSRFLAVSDPRRTVVCATQ
                     GHGEPALDSLEPYSGHAHLRDLLRDAGLEVRVADLDTPGGLGGCNLVLVGGPQGPLPA
                     AQVQAIDRFAGEGGDLLILAGAVILRGKSAIAQHGLEALVARYGVAFGERVVVDPSPM
                     AGATPFLAFTLRDGWGEHPATASLVGQPISLLQVRELVLTAPATFLIQTSDQGWAEAE
                     VAGFGRGELPKFDDGVDRAGPLPVAAAAELGGSRMIVVASSDFALNAMLREDIVYDRG
                     RDLLLNAVGWLTEHEALLGLRPRPREHVKLVLQDDQLARMS"
BASE COUNT          535 a         1451 c         1587 g          587 t
ORIGIN      
        1 cttcgccgcg ctgacgcccg aggacgcggc cgggccgggg cgcgtgctcg ccgaggtcgg
       61 ccccgagctg cgcacgatcg ccgagacggc gaccgacatg cacctgcgcg ccaacgcgag
      121 cctgctgctg ggcaccctct acgaggcgac cggcgacccg cgctcggcga tctcgttcta
      181 ccgtcaggtg gtcgcgctgc tgccgaacga ccccgagccg caccgcgtgc tggcgatggc
      241 gctggcggcc gacaaacaat tcgcggccgc gctgccggag caggagctgg tcgtcaagga
      301 cgaccccgat gacctcgagg cctggctgct gctcggcgag ctcgcggtca aggccggcga
      361 gaaggaccag gcgacccagg cctatggcgc ctacgagctg cgccgcaagg gcctgatcga
      421 cggcatcagc ctcaagaagg ccgacggcac cttcccgctg cccgccgagc agcgggccgc
      481 gtgcgcgcgc gcgctgatcc ccgcgcgcga caacggcacc gcgatcgcgc tgctgtacgc
      541 gctggagatc gagacggacc cgggcgtgcg cgtggcgatc gtcgaggcga tgggcgccca
      601 gcgcctcgcc ggctacaagc cgacgctcga gaccaagctc aagacggaga ccgcccagga
      661 ggtcaaggag gcgatgatct gggccctcgc ggagatccag cgcgacccgc tcgacacccg
      721 cccgggaccg acgccgcaag ggccgggaac cggcgaggac gcgaaggccg gcgatgccgc
      781 tggcgaggcc gctggtgatc cgaaggccgg cgacgtgaag gccgagagcg cgaaggccga
      841 ggacgccaag gccacggacg tgaaggccgc cacgaaggtc gatgacgcga aggcaaccgg
      901 cgcgaaggga caggtccctg cggacaggtc cgccgggaca gacgcgaagg ccggcacacc
      961 ggcgccgtca cgatgagcat gcgtccgccc gcctcgctcg agatcgccgc gctgtcgcac
     1021 cgctacccgg gtgcggccgt acccgcgctc gcgcaggtgg gctttcgcgt cgcgccgggg
     1081 gagcgggtcg ggctgctcgg gcccaacggg gccggcaagt cgacgctgat gcgcctgctc
     1141 tgcggctacc tgccggtgca gcgcgtgccc gggacgcgcg tgcgcgtcgg cgaggtcgag
     1201 gtcgcggccg actcgctgcg cgcgcgccag ctcgtcggct acttgcccga gcaggtgccg
     1261 ctgtatccgg agctgcgggt ccgcgagcac ctcgactttc gcgcgtctat caagggcgtg
     1321 cggcgcaagg cccggccacc ggagatcctt cgcgtcgcgg agcgcaccgg cctgtcgcac
     1381 atgctcgagg tcccgatcca gcagctcagc cgcggctatc gccagcgcgt cgggatcgcc
     1441 gatgcgctgc tcggcgcgcc gccgctggtc gtgctcgacg agccgaccgt cggcctcgac
     1501 cccaaccagg tgctcgagat ccgcgccatg ctgcgcgcgc tcggcgggac gcagacgctg
     1561 atcttctcgt cgcacatttt ggcggaggtc gaggcgctgt gcgaccgcgt cgtcatcctc
     1621 gcgggtggca agtgcgtcgc cgacgaggcg gtcgccgagg cgctgcgcgg cagcgacgtg
     1681 atcgtcgcgt ggatgatcgc gcaggcggac gcggagatcg tattggcgag cgcgctgcgc
     1741 gacatgtccg ccgggacagg tgatttgaca acgctcgcgg acgggctggg cctgaggctc
     1801 gggagcttcg aggcgcgcgg cgacgcggcg cagctgcgga tcacctgtcc cgacgcgcag
     1861 gtcgccgagg cgctcgcgcg ggcgatcggg cgggccagcg cggcggcagg cgtggcgctg
     1921 gcgcggctcg agctcgggcg cggacgactc gaggagcgct tcgcccgggt cacgggggcg
     1981 gtggccgggg catgagcacg tgggacacgc cggtcgagcc gttgcagcgc ccggggccgt
     2041 ggctgcgcgg ggcgtaccac gtcgcgcgcc gcgagctgct gtcgctgttc gtcacgccgc
     2101 tcgcgtacct cgtcggcacg ctgttcctgc tcaatcaggg ctggaacttc gcgctgctgc
     2161 tgcgcgtgct caacgacccg ctcgccgcgc ccgggccggt gatgcagttc tacttcggcg
     2221 gcagcttctt catcttctgg ctgccggtga tcttcatctg cgcggcgctc agcatgcgcc
     2281 tgatcgccga ggagcggcgt cagggcacgc tcgaggcgct gttgacggct ccgctgtcgc
     2341 cgtcccagat cgcgctcggc aagttcgcgg gggcgtacgg cttttacctc gcgctgtggc
     2401 tgccgaccgc gacgttttat ctgctgctgc tcggcgccgg ggcgcggccc gaggccggtc
     2461 cggtgctcgc gggttacctc ggcgtgctgc tcgtcggcgc gagttttttg agcgtgggat
     2521 tactggcgag cgcgctggcc cgcagccagc tggcggcggc gatcagcacc ttcgtgacct
     2581 gcacgatcgc cgtgatggcc gggctgctgt cggagcaggt ccaggcgggc acgacggcgc
     2641 agctgctgca cttcaccagc ctgctcgtga tgatgcagga gctcgcgcag gggatcgtcg
     2701 atccgcgctg ggtgtacctg cacctcgggt tcacggccac gctgctggcg gcgacgatcg
     2761 tcgcgctcaa ccctcgcagg cggcgcgagc acgcggtcca ggtcgtgctg ctggcgctgc
     2821 tgctcgggca cctgacggtg ttcggcgcgc gtcatgggac cagggatgac tggaccgcgg
     2881 gccacgtgta cacgctcagc gatcgcgcgc gcgaggtgct cggcgggcta aagacgccga
     2941 tcgacgtcag cgtgatcgtg ccggcgacga tcggcggcgg ccggcccaac ccgttgctcg
     3001 ccgagctgcg cgaggtgctg gcgcgcatgg gcgccgcggc gccgaacctg cggatccgct
     3061 gggtcgaccc cgaccgcgac cacgcgcagg cgcaggcgag catcgccgag ttcgcgctcg
     3121 gcggccgcga gctggccgac ggcgtggtgc tgctgcgggc cgggcagggg gccagcctgc
     3181 gcaaggcgca cttgttgccc ggcgagctcg tcaccttcgc caccggcccg gacgtgcagc
     3241 agaccggccc gcgggtccgc gagttccgcg gcgaggaggc cctgctctcg cgtttcctgg
     3301 cggtcagtga tccgcggcgc acggtcgtgt gcgcgacgca gggtcacggc gagcctgccc
     3361 tcgacagcct cgagccgtac agcggccacg cgcacctgcg cgacctgctg cgcgacgcgg
     3421 gcctcgaggt ccgcgtcgcc gacctcgaca cgcccggggg gctcggcggc tgcaacctcg
     3481 tgctcgtcgg cggaccgcag ggtccgttgc cggcggcgca ggtgcaggcg atcgatcgct
     3541 tcgcgggtga gggcggcgac ctgttgatcc tcgcgggcgc ggtgatcctg cgcggcaaga
     3601 gtgcgatcgc gcagcacggg ctcgaggcgc tggtcgcccg ctacggcgtc gcgttcggcg
     3661 agcgcgtcgt cgtcgatccg agcccgatgg ccggcgcgac gccgttcctg gcgttcacgc
     3721 tgcgcgacgg ctggggtgag catccggcga ccgcgagcct cgtcggccag ccgatctcgc
     3781 tgttgcaggt gcgcgagctg gtgctgacgg cgcccgcgac gttcctgatc cagaccagtg
     3841 atcaaggctg ggcggaggcg gaggtcgcgg gcttcgggcg cggcgagctg ccgaaattcg
     3901 atgatggcgt cgatcgggcc gggccgctgc cggtcgccgc ggcggcggag ctcggcgggt
     3961 cgcggatgat cgtcgtcgcg tcgtcggact tcgcgctcaa cgcgatgctg cgcgaggaca
     4021 tcgtctacga ccgcgggcgc gacctgctgc tcaacgcggt cgggtggttg acggaacacg
     4081 aggcgctgct cgggctgcgc ccgcggccgc gcgagcacgt caagctggtc cttcaggacg
     4141 accagctcgc gcgcatgagc
//
LOCUS       DASWNJ010001492         2405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_310106, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001492 DASWNJ010000000
VERSION     DASWNJ010001492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310106"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VGB85_24815"
     CDS             <1..198
                     /locus_tag="VGB85_24815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137338.1"
                     /translation="TVLRWARIGVLPAPEIVHRGRRGRLTRWPTHTEAQARWVQGQLD
                     AGQTFDEIAAALAAGRFKPKR"
     gene            complement(208..1080)
                     /locus_tag="VGB85_24820"
     CDS             complement(208..1080)
                     /locus_tag="VGB85_24820"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY0137339.1"
                     /translation="MHAIGRDLIESMFRKAGVEAPTGQYDVDVKRQLEHVDIVAEIGA
                     EHLLVIEDKVHSAEHSNQLQIYARAISALYPQRKRAFLFLKTGDQSSYSAVEADLWTT
                     FLRHDLLAVLRRGAGCQNAIHVDFLAMIEAREAAVQRFRTTPVAAWGPGDPAYVGLYL
                     QLQVLLGDGHWRYVPNPSGGFLGFWWHNDGIEGGEIYLQLEEDKLVVKIWVEDGERRG
                     ELRGLWSRRVVVGIPGFSRPTRFGNGEYMTVATGGDYRIPGSDGRLDLEATVSRLQTV
                     AAALARLVAAPPAA"
     gene            complement(1255..2235)
                     /locus_tag="VGB85_24825"
     CDS             complement(1255..2235)
                     /locus_tag="VGB85_24825"
                     /inference="COORDINATES: protein motif:HMM:NF042779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOPRIM nucleotidyl transferase/hydrolase
                     domain-containing protein"
                     /protein_id="HEY0137340.1"
                     /translation="MGLHAQLLAQDHRYIMIDEPEAFLHPPLARRLGFNLSTLAAERG
                     SCVFAATHSPDFLMGCIESGVGVNIVRLGYHNGVASAHLLPRDELETMMVDPLLRSTG
                     ILGALFHQAAVVVEGDSDRAFYSEVNERLRIHEPGIHPAVMRDCVFLNAQGKATVSRI
                     LEALRRLGVVSCGIVDLDLLKDNGVCKKLLEARGATEAVAESIGNTRASIQRSIADLK
                     ALCLPNGLARATEQERRSVQHFIHEMELHGIFLPEVGPVEGWLAKLGVSAHKNAWIPA
                     IFKAMGPVNTGLKPSPHDVWKFIRRIGAYLDVQATPAVVPDPEADPPADA"
     gene            2200..2355
                     /locus_tag="VGB85_24830"
     CDS             2200..2355
                     /locus_tag="VGB85_24830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137341.1"
                     /translation="MILRQELGMQTDIRADTVAKVVEGPRACEEFAGLGIEAVLDTGG
                     GPALGED"
BASE COUNT          406 a          753 c          860 g          386 t
ORIGIN      
        1 acggtgctgc gatgggcacg gatcggcgtc ttaccggcgc cggagatcgt tcatcgcgga
       61 cgacgtggcc gcctgacccg ctggcccacg cataccgagg cccaggctcg atgggtgcaa
      121 ggtcagctcg acgccgggca gacctttgac gagatcgcgg ctgcgctcgc cgcaggccgc
      181 ttcaagccga agcggtgagc gtgcgggtca cgctgccggc ggcgcggcga ccagccgagc
      241 gagggcggcg gcgacggtct gtagtcggga aaccgtcgcc tcgaggtcga ggcggccgtc
      301 ggatcccggg atgcgatagt cgccccccgt ggcgaccgtc atgtattcgc cgttgccgaa
      361 gcgcgtcggc cgggagaaac cggggatgcc cacgacgacg cggcgggacc acagtccacg
      421 aagctcaccg cggcgttcgc cgtcctcgac ccagatcttg accacgagct tatcctcttc
      481 gagctggaga tagatctcgc cgccctcgat gccgtcgttg tgccaccaaa acccgaggaa
      541 gccgccggag gggttgggca cataacgcca gtggccgtcc ccgagaagga cttggagctg
      601 aagatacagg ccgacgtacg cggggtcgcc aggaccccac gcggcgaccg gcgtcgtcct
      661 gaagcgctgt acggccgcct cgcgggcctc gatcatggcg aggaagtcga catggatcgc
      721 gttctggcag ccggcgccgc gccgcaggac cgcgagcaga tcgtggcgca ggaaggtcgt
      781 ccatagatcg gcctccaccg cgctatacga ggactgatcg ccggtcttga ggaagaggaa
      841 cgcgcgcttg cgctgcgggt agagggccga gatcgcgcga gcatagatct ggagctggtt
      901 ggagtgctcg gccgagtgca ccttgtcctc gatgacgagc aggtgctccg cgccgatctc
      961 cgcgacgata tcgacgtgct cgagctgccg cttgacatcc acgtcgtact ggccagtcgg
     1021 cgcctcgacc ccggctttgc ggaacatcga ctcgatcagg tcccgcccga tcgcgtgcat
     1081 cgcggggtcc gtcgcggcgt gacggctgtc agcccatgcg atacaccagc agagcgccgc
     1141 gtcctgcgac agttccgagg tcgcgaagtc gaagagattg gggccacgcg tggtcatcgc
     1201 gccagaatac acgccgagag gtcgcttgcg gcggcgaagc gcggcgcgaa cgggtcaagc
     1261 gtccgccggt gggtcggctt cggggtccgg cacgaccgcg ggggtggcct gtacatcgag
     1321 gtaagcgccg atgcggcgaa tgaacttcca gacgtcgtgg gggctcggtt tcaagccggt
     1381 gttcaccggc cccatcgcct tgaagatcgc ggggatccag gcgtttttat gggcgcttac
     1441 gccgagcttg gccagccacc cctcgacggg ccccacctcg ggcaggaaga tgccgtggag
     1501 ctccatctcg tggatgaagt gttgtacgga cctgcgctcc tgttccgtgg cgcgagcgag
     1561 gccattcggg aggcaaagcg ccttgaggtc agcgatactt cgctgaatgc tcgcccgggt
     1621 gttaccgatg gactcggcca cggcctccgt cgcgcccctc gcctcgagca gcttcttgca
     1681 aacgccgttg tccttgagca ggtcgaggtc gacgatgccg caggagacga cgccgaggcg
     1741 ccgtagcgct tcgaggatgc gcgagacggt cgcttttccc tgcgcgttga ggaagacgca
     1801 gtcgcgcatc accgctggat gaatgccggg ctcgtggatg cgcaggcgct cgttgacctc
     1861 gctgtagaac gcgcggtcgc tgtcaccctc gacgaccacg gcggcctggt gaaatagagc
     1921 gccgaggatg ccggtcgagc gcagcagcgg atcgaccatc atcgtctcaa gctcgtcccg
     1981 gggcagcagg tgggccgacg cgacgccgtt gtggtagccg aggcggacga tgtttacgcc
     2041 gacaccggat tcgatgcagc ccatcagaaa gtccgggctg tgggtcgccg cgaacacgca
     2101 ggagccgcgc tcggccgcga gggtgctcag gttgaacccc agtcggcggg cgagcggggg
     2161 gtgcaggaac gcctccggct cgtcgatcat gatatatcga tgatcctgcg ccaagagctg
     2221 ggcatgcaga ccgatatacg tgcggacacc gtcgctaaag ttgtcgaggg gccgcgcgcc
     2281 tgcgaagaat tcgcgggcct cggtatcgag gcggtcctcg ataccggggg ggggccggcg
     2341 ctcggagagg actagctgcg ccgtacggag gtcgaggatg tcgacacaga tgtgttggtg
     2401 gaagg
//
LOCUS       DASWNJ010001493         2705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_558073, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001493 DASWNJ010000000
VERSION     DASWNJ010001493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558073"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1606)
                     /locus_tag="VGB85_24835"
     CDS             complement(<1..1606)
                     /locus_tag="VGB85_24835"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0137342.1"
                     /translation="MKRSKLSHPSRRSIPGLVAFTLALASCSGEGTGTSTGDTSGSSG
                     STDMPGSATDVEPTGGATEPGTSATGSTGSTGITSESSSETGSSESSGDTSSGTSNPG
                     SSDTSSTDTGDTGDTGDTGDTGDTSDTDDTGVLPEEFIKVQIVALNDFHGNLEPPAGS
                     NGQIAVGPNMKVDVGGAAFLADHVAALRAENPNTVVVSAGDLIGGSPLVSALFHDEPT
                     IEVMNQIGLDYNAVGNHEFDHGVDELLRMQEGGCHPLDGCLDGTPFPGASFQFLAANV
                     LTSLDPVKTLFPAFAVHEVAGVKIGFIGMTLEGTPGIVTPVGNEGLIFLDETERANAH
                     VPTLLAMGVEAIVVLIHEGGFQGSFYDECAGISGPIVDIVEGLDDEIDVVITGHTHQA
                     YNCIIADKIVTSAASHGRVLTDIDLEISTWTGQVTAATASNVLVTRDMADPEVAAFVA
                     EYKQLAAPLANQQLGTITATLNRYAPMMKPGLSTMGAVLADAHLAATAPAMLGGSQMA
                     FMNPGGVRADLLYPASNGEPKDGIVSY"
     gene            complement(1719..2600)
                     /locus_tag="VGB85_24840"
     CDS             complement(1719..2600)
                     /locus_tag="VGB85_24840"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0137343.1"
                     /translation="MLSRRSFTAALSAAPLACASAGAVRRTYIERRAKHTTQLGKRGP
                     SPGKWRPQAVPAGVTELRYPSAGRELLAWFAAPPLASGARAPALIYFHGEFSFAAWDF
                     NKVRPFLDAGYCVMTPTLRGENGNSGEFELLYGEVEDACAAVAWLAARPEVDPAKIYT
                     LGHSVGGGLSALLALQPALPVACTASVGGIYTTATFARWARDADTRDLVRFDPAVRDE
                     VELRVLGPHLAELQRPHRAYVGRHDAGILDNARGLGSHAASINAPFRLTEVAGDHASS
                     LDPGIAAFLADLRAGVT"
BASE COUNT          376 a          942 c          961 g          426 t
ORIGIN      
        1 cgtagctgac gatgccgtcc ttgggctcgc cgttggacgc cgggtacagc aggtcggcac
       61 gcacgccgcc cgggttcatg aacgccatct gggacccgcc gagcatcgcc ggcgcggtcg
      121 cggcgaggtg cgcgtcggcg agcacggcgc ccatcgtcga caggcccggc ttcatcatcg
      181 gcgcgtagcg gttgagcgtc gccgtgatcg ttccgagttg ttggttcgcc agcggcgcgg
      241 cgagctgctt gtactcggcg acgaaggcgg cgacctcggg gtcggccatg tcgcgggtga
      301 cgagcacgtt gctggcggtc gccgcggtca cttgacctgt ccacgtgctg atctcgaggt
      361 cgatgtcggt cagcacgcgc ccgtgcgacg ccgcgctggt gacgatcttg tcggcgatga
      421 tgcagttgta ggcctggtgc gtgtggccgg tgatcacgac gtcgatctcg tcgtcgaggc
      481 cctcgacgat gtcgacgatc ggccccgaga tgccggcgca ctcgtcataa aagctgccct
      541 gaaacccgcc ctcgtggatc agcacgacga tcgcctcgac gcccatggcg aggagcgtcg
      601 ggacatgggc gttggcccgc tcggtctcgt cgaggaagat gagcccctcg ttgccgaccg
      661 gcgtcacgat ccccggcgtg ccctcgagcg tcatgccgat gaagccgatc ttgacgcccg
      721 cgacctcgtg caccgcgaac gccgggaaca gggtcttcac cgggtcgagg gaggtcagca
      781 cgttggccgc caggaactgg aagctcgcgc ccgggaacgg cgtgccgtcg aggcagccgt
      841 cgagcgggtg gcagccgccc tcctgcatgc gcaacagctc gtcgacgccg tggtcgaact
      901 cgtggttgcc gaccgcgttg tagtcgagcc cgatctggtt catcacctcg atcgtcggct
      961 cgtcgtggaa cagcgccgac accagcggcg acccgccgat caggtcgccc gccgagacga
     1021 cgaccgtgtt cgggttctcc gcgcgcagcg ccgcgacatg gtcggccaaa aacgccgcgc
     1081 ccccgacgtc gaccttcatg ttcgggccga ccgcgatctg cccgttgctg cccgccggcg
     1141 gctcgaggtt gccgtgaaag tcgttgagcg cgacgatctg caccttgatg aattcttcag
     1201 gcagcacgcc ggtatcgtcg gtgtcgctgg tatcacctgt atcgccggtg tcgcccgtat
     1261 cgcccgtgtc gcccgtgtcc gtgctgctcg tgtccgagct gcccgggttg gacgtgccgc
     1321 tcgaggtatc cccgctgctc tcgctcgatc cggtctcgct gctcgactcg ctggtgatgc
     1381 cggtgctgcc cgtgctgcct gtcgcgctcg tgcccggctc ggtggcgccg cccgtcggct
     1441 cgacgtccgt ggcgctgccc ggcatatcgg tgctgccgga ggaaccgctg gtgtcgcccg
     1501 tgctcgtgcc ggtgccttcg ccggagcagc tcgccagcgc gagcgtgaat gcgacgaggc
     1561 ccgggatgga gcgacgcgag gggtgcgaga gcttcgatct tttcatcgga taattctttt
     1621 tgcggaaagc ggctgccgtc atatcagaaa ataccccgct cgcgcgcagc tccccgcgtg
     1681 gcgccggcag acccgggtca cgcgcggccg tgtggcgctc aggtcacacc ggcgcgcagg
     1741 tcggcgagga aggccgcgat gccgggatcc agcgagctcg cgtgatcccc cgccacctcg
     1801 gtcaggcgaa acggcgcatt gatcgaggcg gcgtggctgc ccagcccgcg cgcgttgtcg
     1861 aggatgccgg cgtcgtggcg gccgacgtac gcgcggtgcg ggcgttgcag ctccgcgagg
     1921 tgcgggccga ggacgcgcag ctcgacctcg tcacgtacgg cggggtcgaa gcgcacgagg
     1981 tctcgcgtgt ccgcgtcgcg ggcccagcga gcaaaggtcg cggtcgtgta gatcccgccg
     2041 acgctggccg tgcacgcgac cggcagcgcc ggctgcaacg cgagcagggc agagagcccg
     2101 ccgccgacgc tgtgcccgag cgtgtagatc ttcgcggggt cgacctcggg ccgcgccgcg
     2161 agccaggcca ccgcggcgca cgcgtcctcg acctcaccat ataacagctc gaattccccc
     2221 gagttaccgt tctcgccgcg cagcgtcggc gtcatcacgc aatagcccgc gtcgaggaat
     2281 ggtcgcacct tattaaaatc ccaggcggcg aacgagaact cgccgtggaa gtagatcagc
     2341 gccggggcgc gggcgccgga cgccagcggc ggcgccgcga accacgcgag caactcgcgg
     2401 cccgcggacg gataacgaag ctcggtcacg ccggcgggca ccgcctgcgg gcgccacttg
     2461 ccgggcgagg gcccgcgctt gccgagctgc gtcgtgtgct tcgctcgccg ctcgatgtac
     2521 gtacgccgca ccgcccccgc gctcgcgcag gcgagcggcg cggccgacag cgcggcggtg
     2581 aagctccggc gggacagcat ggcctgcgac ctcgacgcgc ccgctggacg ggcgcgccgc
     2641 gttcacgccg acttgcggcg gcgcagcagc agcagcagca gcggcgtcag cagccccatg
     2701 ggcgc
//
LOCUS       DASWNJ010001494         4394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_274893, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001494 DASWNJ010000000
VERSION     DASWNJ010001494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274893"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="VGB85_24845"
     CDS             complement(<1..919)
                     /locus_tag="VGB85_24845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137344.1"
                     /translation="MKPRADTTRTAGAALGMLGMLGMLGMLGVLGMLSMLAPGCSSLA
                     PADTPQGCTIDADCQTLGHVCGEGVCYDPNLPPRSHIGLDVRGGGQATDFRVELRGED
                     SAVLRIDRSPIRYSVSLDDRKDTPGIRDYIEISLRETFGTGNAKESVALAGVLSLSQA
                     SRLGRDPVTVENRRIDLFDAMGVPLDEADAKLTLPWARYDRDPLGNDIPLLLGVMADM
                     TKKDTLEVHRGPVFRQLVRPQLAGVGLHGFTIQTRRECHRKIRGVVINGATGKGIGHV
                     EVKFEHARHEPGDGDFCDPEPEAGTPALCS"
     gene            complement(916..1752)
                     /locus_tag="VGB85_24850"
     CDS             complement(916..1752)
                     /locus_tag="VGB85_24850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137345.1"
                     /translation="MSRRHIALVLACAAGCVLPDRGIIISDERVQNKHPVRFVEPTPV
                     SNEASNLCLAVLMELEMPTSACQPSDPTVALPHFLDPDYKEGDIRPYAFCSCDPPKVD
                     TRKLAAVTLYVEDRADDIDAGLDPIYAALQLDLRPDESEPQKTVKYQTHVNPSAALVE
                     PDPKYLKYAPPARPNEAAGRELRELNLGFSDNDQIDLCNGVGSDPLPRGYHTLRVIVT
                     DAPWFTPPAPDNMPNQERVQQAGVPDLAEGATYDTLTYTFHCGDKTNEDDDCENRCKV
                     PE"
     gene            complement(1749..3584)
                     /locus_tag="VGB85_24855"
     CDS             complement(1749..3584)
                     /locus_tag="VGB85_24855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY0137346.1"
                     /translation="MPHLQYPGPDGTTVRFKLLRRLTSIGSSRECDVVITGTDIAATH
                     CTIEHEAGNFKLESASRSAPFYVAGKKLREHNLRHGDVFVVGPVELTFAAIDPQPAAN
                     DPESAAKVQIEAMRQLQRFSELLLRAESSLDGLLDELIDQVVSLTSASKGFLVLAEGD
                     RYEVRVARGLDRQPVDDPEQLLSDKIIREVITSREPQIISDAYHDTRFQNSMSVINLK
                     LSSVMCVPLIVRGELLGLIYVGNNSVVNLFKQSQLETLRVYAAQAALFIKNAKLVNEL
                     RSDAVGLQKRLDDVKFGQIIGSCPQMIEVYKRVEKVAGTDISVLVTGETGTGKELIAQ
                     EIHNRSPRAKGPLVTVNCGAIPENLIESELFGHVKGAFTGAVGNQIGKFQAAGGGTIF
                     LDEVGELPLQLQVKFLRVLQERQVQRVGETKTTPIDVRVVAATNRDLTKEVAAGRFRE
                     DLYHRLNVIDIHLPPLRERGNDVALIARYFVTRYAREYGAKIDAAHPFDPSGERALLR
                     FNWPGNIRQLENHIKKALVLADGPALTARDLDLEIDMPGSAGAPIAIAAPEQILPLAE
                     ARELWQRDYINQVLALNAGNRTKTARDLGVDPRTIFRHLEKEEKK"
     gene            complement(3646..>4394)
                     /locus_tag="VGB85_24860"
     CDS             complement(3646..>4394)
                     /locus_tag="VGB85_24860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137347.1"
                     /translation="LLANSPDWQPPPEIHSRQFYALYNGLREQRDRAQLRECVGERAS
                     CEADVQERKAELTRLQNDYTLLKKAFGEQEVEVREKVARNRAVAMIPFGVGHFYNGRR
                     ALGAAFLSGELVFGSVALGLFIARSFNCTRTKGFQVGSLQCNHSGNGEDIVRRRNAEQ
                     AMGLFFLGAVALDIVLAQVTFRPYLTVKSTRVRRQDLGAEPDDKGRRGGRAGKEPRPS
                     SRTRSHDILHVAPAPAYIPGGGGLGVTLRF"
BASE COUNT          608 a         1341 c         1694 g          751 t
ORIGIN      
        1 gggagcacag cgcgggcgtg ccggcctcgg gctcggggtc gcagaagtcg ccgtcgccgg
       61 gctcgtgacg ggcgtgttcg aacttgacct cgacatgccc gatacccttg ccggtggcgc
      121 cgttgatgac gacgccgcga atttttcgat ggcactcgcg tcgggtctgg atcgtgaagc
      181 catgcaggcc gacgccggcg agctgcgggc gcacgagctg gcggaagacg gggccacgat
      241 ggacctcgag tgtgtccttc ttggtcatgt cggccatcac accgagcagc agcgggatgt
      301 cgttgccgag cgggtcgcgg tcgtagcggg cccacggcag cgtcagcttg gcgtccgcct
      361 cgtcgagcgg gacgcccatc gcgtcgaaga ggtcgatgcg gcggttttcg acggtgacgg
      421 ggtcgcgtcc gagccgcgag gcctggctca gcgacaggac gcccgcgagc gcgacgcttt
      481 ccttggcgtt gccggtgccg aaggtctcgc gcagggagat ctcgatgtag tcgcggatgc
      541 cgggagtgtc cttgcggtcg tcgaggctga cgctgtaacg gatcggcgag cgatcgatgc
      601 gcagcaccgc cgagtcctcg ccgcgcagct cgacgcggaa gtcggtcgcc tggcccccgc
      661 cgcgcacgtc gagcccgatg tggctgcgcg gcggcaggtt ggggtcgtag cacacgccct
      721 cgccgcacac gtggccaagg gtctggcagt cggcgtcgat ggtgcagccc tggggggtgt
      781 cggcgggcgc gagcgaggag cagccggggg cgagcatgct gagcatgccg agcaccccga
      841 gcatgccgag catgccgagc atgccgagca tgccgagcgc cgcgccggcg gtccgcgtgg
      901 tgtcggcccg gggcttcact ctggcacctt gcaccggttc tcgcagtcgt cgtcctcgtt
      961 ggtcttgtcg ccgcagtgga aggtgtatgt cagggtgtcg taggtcgcgc cctcggcgag
     1021 gtcgggcacg ccggcctgct ggacccgttc ctggttgggc atgttgtcgg gggctggcgg
     1081 cgtgaaccac ggcgcgtcgg tgacgatgac gcgcagcgtg tggtagccgc ggggcagcgg
     1141 gtccgagccg acgccgttgc acaggtcgat ctggtcatta tccgagaaac caaggttgag
     1201 ctcgcgcagc tcacggccgg cggcctcgtt cgggcgggcc ggcggggcgt acttgaggta
     1261 cttggggtcg ggctcgacca gcgcggcgct cggattgacg tgggtttggt atttgacggt
     1321 cttttgcggc tcgctctcgt cgggccgcag atcgagctgg agggcggcgt agatcgggtc
     1381 gaggccggcg tcgatgtcgt cggcgcggtc ctcgacatag agcgtgacgg cggcgagctt
     1441 gcgggtatcg accttcggcg ggtcgcacga gcaaaaggcg tacggcctga tgtcgccctc
     1501 cttgtagtcg gggtcgagga agtgcggcag cgcgaccgtc gggtcactcg gctggcacgc
     1561 gctggtcggc atctccagct ccatcaggac cgcgaggcac aggttgctgg cctcgttgct
     1621 gaccggtgtc ggctcgacga agcgcaccgg gtgcttgttc tgcacgcgct cgtccgagat
     1681 gatgatgccg cggtccggca gcacgcagcc ggccgcgcac gcgaggacga gagcgatgtg
     1741 ccggcggctc acttcttctc ttccttctcg aggtggcgaa agatcgtgcg tgggtcgacg
     1801 ccgaggtcgc gggcggtctt ggtgcggttg ccggcgttga gcgcgaggac ctggttgatg
     1861 tagtcgcgct gccacagctc acgcgcctcg gccaatggga ggatctgctc cggggccgcg
     1921 atcgcgatcg gggcgccggc gctgccgggc atgtcgatct cgaggtcgag gtcgcgggcg
     1981 gtcagcgcgg ggccgtcggc gagcacgagc gcctttttga tgtggttctc gagctggcgg
     2041 atgttgccgg gccaattgaa gcgcagcagg gcccgctcgc cgctggggtc gaaggggtgg
     2101 gcggcgtcga tcttggcccc gtactcgcgc gcgtagcggg tgacgaagta gcgggcgatc
     2161 agggcgacgt cgttgccgcg ctcgcgcagc ggcggcaagt ggatgtcgat cacgttgagg
     2221 cggtgataga ggtcctcgcg gaagcggccg gcggcgacct ccttggtgag gtcgcggttg
     2281 gtcgccgcga cgacgcggac gtcgatcggg gtggtcttgg tctcgccgac gcgctggacc
     2341 tggcgctctt gcagcacgcg caggaacttg acctgcagct gcagcggcag ctcgccgacc
     2401 tcgtcgagga agatcgtgcc gccgccagcc gcctggaact tgccgatctg gttgccgacg
     2461 gcgccggtga aggcgccctt gacgtggccg aacagctccg actcgatcag gttctcgggg
     2521 atggcgccgc agttgacggt gacgagcggg cccttggcgc gcggcgaccg gttgtggatc
     2581 tcctgggcga tcagctcctt gccggtgccg gtctcgccgg tgacgagcac gctgatatcg
     2641 gtgccggcga ccttctcgac ccgcttgtag acctcgatca tctgcgggca cgagccgatg
     2701 atctggccga acttgacgtc gtcgaggcgc ttttgcaggc cgacggcgtc gctgcgcagc
     2761 tcgttgacga gcttggcgtt cttgatgaag agggcggcct gggcggcgta gacccgcagg
     2821 gtctcgagct ggctctgctt gaacaggttc acgacgctgt tgttgccgac gtagatcagg
     2881 ccgagcagct cgccgcggac gatcagcggc acgcacatga cgctcgacag cttgaggttg
     2941 atcaccgaca tgctgttttg aaagcgcgtg tcgtgatagg cgtccgagat gatctgcggc
     3001 tcgcgcgagg tgatcacctc gcggatgatc ttgtcggaga gcagctgctc ggggtcgtcg
     3061 accggctggc ggtcgaggcc gcgggcgacg cggacctcgt agcggtcgcc ctcggcgagc
     3121 acgaggaagc ccttgctcgc gctcgtcagc gagacgactt gatcgatcag ctcgtcgagc
     3181 aggccgtcga gggagctctc ggcgcgcagc agcagctccg agaagcgctg cagctggcgc
     3241 atcgcctcga tctggacctt ggcggcgctc tcggggtcgt tggcggccgg ctgggggtcg
     3301 atcgcggcga aggtgagctc gaccgggccg acgacgaaga cgtcgccgtg gcgcaggttg
     3361 tgctcgcgca gcttcttgcc ggcgacgtaa aacggcgcgc tgcggctggc cgactcgagc
     3421 ttgaagttac cggcctcgtg ctcgatcgtg cagtgcgtcg cggcgatgtc ggtgccggtg
     3481 atcacgacgt cgcactcgcg cgagctgccg atcgaggtca ggcggcggag cagcttgaag
     3541 cggacggtcg tgccgtcggg gcccgggtat tgcagatggg gcatggaggg tggtgggctc
     3601 gagtggacta gaagcgcagc gtcaggctag aagcgcagcg tcaggctaga agcgcagcgt
     3661 cacgccgagg ccgccgccgc cggggatgta ggccggggcg ggcgcgacgt gcaagatatc
     3721 atgggagcgc gtgcggctgc tcggacgcgg ctccttgccg gcacggccgc cgcgacgacc
     3781 cttgtcgtcg ggctccgcgc cgaggtcctg acggcgcacg cgcgtcgact tgaccgtcag
     3841 ataggggcga aaggtgacct gcgcgagcac gatgtcgagg gcgacggcgc cgaggaagaa
     3901 caggcccatc gcctgctcgg cgttgcgccg gcggacgata tcctcgccgt tgccggagtg
     3961 gttgcactgg agcgagccga cctggaagcc cttggtgcgc gtgcagttga agctgcgggc
     4021 gatgaacagg ccgagcgcga cgctgccgaa caccagctca ccggacagga acgcggcgcc
     4081 gagggcccgg cggccgttgt agaagtggcc gacgccgaac gggatcatcg cgacggcgcg
     4141 gttgcgggcg accttctcgc ggacctcgac ctcctgctcg ccgaacgcct tcttgagcag
     4201 ggtgtagtcg ttttgcaggc gggtcagctc ggccttgcgc tcctggacgt cggcctcgca
     4261 ggaggcgcgc tcgccgacac actcgcgcag ctgggcccgg tctcgctgct cacgcaggcc
     4321 gttgtagagg gcgtagaact ggcggctgtg gatctccggc ggcggctgcc agtcgggcga
     4381 gttggcgagc aggc
//
LOCUS       DASWNJ010001495         2882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_378252, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001495 DASWNJ010000000
VERSION     DASWNJ010001495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378252"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1381
                     /locus_tag="VGB85_24865"
     CDS             <1..1381
                     /locus_tag="VGB85_24865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137348.1"
                     /translation="MHMRARYLYDRSVLFMVSVALLGGCGDDGPAMTGATETGSTGTS
                     TGDVDPTSTGTPTTDPTTTTDPTTTTTTDTTTTSDTTTTTDTTTTTTTTDTTTTTDTT
                     TDTTTDTTGGTSSSGSDTSTGGGVCGDGVVDGVEGCDDGPDNADNAACTSTCAVAVCG
                     DSLLQEGVEGCDDGAETATCNADCSVSACGDGVVNMAAEELCDEGGESEVCNTDCTLS
                     SCGDGVLNASAGEVCDDGDVVDGDGCSSTCELEMVEVGDIFLGGSSFANVKNALDAMG
                     VTYTEQAQMFIAPGAGDVIILADDGGLEPPPDYTPHMNAGKHLLVIGGSGTQEFVSFV
                     GTYFKVEANGGNWHTADDCDPDWTKGPAHPITAMMPATHTFIDSAISYHMVHLLDAVQ
                     IPDVALLGRTCHNAPNNHVLAVRKYPGGGSFTFMALDLGAYNDAESQATFVVPFLTGY
                     FDWLESGAP"
     gene            complement(1560..2039)
                     /locus_tag="VGB85_24870"
     CDS             complement(1560..2039)
                     /locus_tag="VGB85_24870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137349.1"
                     /translation="MTDDQRLTYAGIYVLKKMDLKPADGGIEMPVVLDSMMASLDEVL
                     ERLVMQGHLEIDRKRQRYKITKAGYKYIGALIDEAEAVISEFDEWETADMVAELKTRN
                     LDPLRARFLWGWYQDEFDDLVQFQERRGIDPVEPYWANFVTSSEFYDNLALDLEDPN"
     gene            complement(2064..2591)
                     /locus_tag="VGB85_24875"
     CDS             complement(2064..2591)
                     /locus_tag="VGB85_24875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137350.1"
                     /translation="MPPVTTLTALPFPERDPWELLGLDPGRSEPDLDHDTSGYCRLPA
                     VTLEDAAGGRQPLSGVLLLGLHSRDDAEPLADDVELEFVLRDGDETYSAMTRLSEFLR
                     LRLAGLVRDASTIVLALCNPQSATVARPPGVGDRPVYYADGDVTAWMQRRPGAHGWTP
                     AEVEIILAAERWHRR"
     gene            complement(2597..>2882)
                     /locus_tag="VGB85_24880"
     CDS             complement(2597..>2882)
                     /locus_tag="VGB85_24880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137351.1"
                     /translation="MFMPGYTPMYRQPYTTSPAARGALASHRSTYRAANPAKFQKASG
                     SGRTYGKTGGSFGSGNSTPTRSSGGGRVGGGRFGIKASKRPAGRKMVHLS"
BASE COUNT          456 a          985 c         1012 g          429 t
ORIGIN      
        1 aatgcatatg cgagctcgat atctgtacga tcgttcggtc ctgtttatgg tctccgtggc
       61 gctgctgggc ggctgtggtg acgatggccc cgccatgacc ggcgccaccg agaccggctc
      121 gaccggcaca tccaccggcg acgtcgaccc gacgagcacc ggcaccccga cgacggaccc
      181 gacgaccacc accgacccga cgacgacgac aaccaccgac acgaccacca ccagcgacac
      241 gacgacgacc accgacacga ccaccacgac gacgaccacc gacacgacga cgaccaccga
      301 cacgaccacc gacacgacga ctgacacgac cggtggcacc agctcgtcgg gcagcgacac
      361 gagcaccggc ggcggagtgt gcggcgacgg cgtggtcgac ggcgtcgagg gctgtgatga
      421 cggtccggac aacgccgaca acgccgcatg caccagcacc tgcgcggtgg cggtctgcgg
      481 cgactcgctg ctgcaggagg gcgtcgaggg ctgcgacgac ggcgcagaga cggcgacctg
      541 caacgccgac tgcagcgtct ccgcctgcgg cgacggcgtc gtcaacatgg cggccgagga
      601 gctgtgcgac gagggcggcg agtcggaggt gtgtaacact gactgcacgc tgtcgagctg
      661 cggcgacggg gtgctcaacg ccagcgcggg cgaggtctgc gacgacggcg acgtggtcga
      721 cggcgacggg tgctcgagca cctgtgagct cgagatggtc gaggtcggcg acatcttcct
      781 cggcgggagc tcgttcgcca acgtgaagaa cgcgctcgac gcgatgggcg tgacgtacac
      841 cgagcaggcg cagatgttca tcgcgccggg cgcgggcgac gtcatcatcc tcgccgacga
      901 cggcggcttg gaaccgccgc cggactacac gccgcacatg aacgccggca agcacctgct
      961 ggtgatcggc ggctcgggca ctcaggagtt cgtcagcttc gtcggcactt acttcaaggt
     1021 cgaggccaac ggcggcaact ggcacaccgc cgacgactgc gaccccgact ggaccaaggg
     1081 ccccgcgcac ccgatcaccg cgatgatgcc ggcgacccac acgttcatcg actccgcgat
     1141 cagctatcac atggtccacc tgctcgacgc cgtgcagatc ccggacgtcg ccctgctcgg
     1201 ccgcacctgt cataacgcgc cgaacaacca cgtgctcgcg gtccgcaagt acccgggcgg
     1261 cggcagcttc acgttcatgg ccctcgacct cggcgcctac aacgacgccg agagtcaggc
     1321 cactttcgtc gtgccgttcc tgaccggcta cttcgactgg ctcgagtccg gcgcgccgtg
     1381 agccgggatc gctgactcgc cgcgcgagtc agcgatgcct gtccgccagc gccgcggcgc
     1441 tggcggcctg tcctcggcct cctgcgggcg ggcgaggcga cgccgcgggt gtcctcgacg
     1501 aagccgtgac gacttcttcg cgctcgcctt cttcttcccg ctctcgggcc cacccggcct
     1561 cagttcgggt cttcgaggtc gagcgccagg ttgtcgtaga actcgctgct ggtgacgaag
     1621 ttggcccagt agggttcgac ggggtcgatg ccgcggcgct cctggaactg cacgaggtca
     1681 tcgaactcgt cctgatacca gccccagagg aagcgggcgc gcagcgggtc gaggttgcgg
     1741 gtcttgagct cggcgaccat atcggcggtc tcccactcgt cgaactccga gatgacggcc
     1801 tcggcctcgt cgatgagggc gccgatatat ttgtagccgg ccttggtgat cttgtagcgc
     1861 tggcgcttgc ggtcgatctc gaggtggccc tgcatcacca ggcgctcgag cacctcgtcg
     1921 agggaggcca tcatcgagtc gaggaccacg ggcatctcga tgccgccgtc ggccggcttg
     1981 aggtccatct tcttgagcac gtagatgccc gcgtaggtga gtctctggtc gtcggtcacg
     2041 gatctcggca taggggtcga tcctcagcgc cggtgccagc gctccgctgc gagaatgatc
     2101 tccacctcgg cgggtgtcca gccgtgcgcg ccggggcggc gttgcatcca cgccgtcacg
     2161 tcgccgtcgg cgtagtagac cgggcggtcg ccgacgcccg ggggtcgcgc gacggtcgcg
     2221 gactgcgggt tgcacagcgc cagcacgatc gtcgaggcgt cgcgcaccag accggccaga
     2281 cgcaggcgca ggaactccga caggcgcgtc atcgccgagt aggtctcgtc gccgtcgcgc
     2341 agcacgaatt ccagctcgac gtcgtcggcc agcggctcgg cgtcgtcgcg gctgtgcagg
     2401 ccgagcagca gcacgcccga gagcggctgg cgcccgccgg ccgcgtcctc gagggtcacg
     2461 gcgggcaggc gacagtagcc ggaggtgtcg tggtccaggt cgggctcgct ccgaccgggg
     2521 tcgagcccga gcagctccca cgggtcacgc tccggaaacg gcagggccgt gagcgtggtg
     2581 accggtggca tgtcctttag gacaggtgga ccatcttgcg gccggcgggg cgcttgctgg
     2641 ccttgatgcc gaagcgaccg ccgccgacgc gtccgccgcc gctcgagcgg gtcggggtcg
     2701 agttcccgga gccgaacgag ccgccggtct tgccgtaggt gcgaccggag ccggaggcct
     2761 tctggaactt ggccgggttg gcggcgcggt aggtgctgcg atgcgacgcg agggcgccgc
     2821 gggcggccgg gctggtcgtg tacggctggc ggtacatcgg ggtgtagccg ggcatgaaca
     2881 ta
//
LOCUS       DASWNJ010001496         4807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_62749, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001496 DASWNJ010000000
VERSION     DASWNJ010001496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62749"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..975)
                     /locus_tag="VGB85_24885"
     CDS             complement(202..975)
                     /locus_tag="VGB85_24885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137352.1"
                     /translation="MRLTSMLLLLSTGLACGPDKSDTETTGGTTGASTGATDTTGGES
                     GVPTGDGPTGSSGDSSAGGTGTGTDATGTGTTSGPVDGCEALQTQEQCEQDPDCMAVV
                     GEAFDFAGCMPGQTFIACMPMMACDSNIQDICEEEPGMEVYRLPNGCVPPGFKACVPG
                     DFPLCGDTACEQLPQQGCLANPDCTAIMGAPHIEQDGEACVDFAMQEFLACMQGDVAC
                     PPVIVSVCPEGQPNMVFDTGSGCIPPGFMPCDMPAPDCQ"
     gene            1760..3073
                     /locus_tag="VGB85_24890"
     CDS             1760..3073
                     /locus_tag="VGB85_24890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0137353.1"
                     /translation="MNRQASTAAVIAPNAPADLDALPELRALEAEVAPVELTETGEST
                     GFLAPYFRDLSNVDVMSRDEELQAAIRLTNLRKSYWKSILSYPPFIDGICDLARAELP
                     AETCPVEALDAMKKASRNLRDRDLLVHQKAYEAACEQLTLAMAEADVDGVVSDRVLAD
                     LTSVEAGCNEGLSMKVKLPRKGSLPFMSYVAAARRNQQALWLAKNAFVKANLRLVVTI
                     ARRFNHGRLPLQDLIQEGNIGLMKAVDRFDHRKGFRFSTYASWWIRHAVTRAIADKAR
                     AVRLPVHMIEAYNKVSKARREFEALHGRRPTDAELATATGVSAERIQRMHYSLVENPV
                     SLDQPLSEDSGLTLLDAVEDTNLALPSELMDNELMMGQLREVFETLSPMEADILRKRM
                     GLDDNQEMTLKEIGARYSLSRERNRQVQEQALAKLRGEFKRRALM"
     gene            3267..3878
                     /locus_tag="VGB85_24895"
     CDS             3267..3878
                     /locus_tag="VGB85_24895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137354.1"
                     /translation="MATRRIVASPPAPVAAPTQPAPAPTPAPAPATSSAKPRRTVGSP
                     EPRTGTLGTLFTELDQAIGDGRMEKVRSMLRADRFQLFSEVGDDKVTAVIKSQTNASL
                     FYACSVDKGGRYMCCTQNLVRCGGLKGNKPCKHLLVMLVGLAQGQQLDPGVALQWARA
                     TKNKAPSLDKDSMSATFIRYKGAEAGEVDWRPTETIPEDYYAF"
     gene            3893..>4807
                     /locus_tag="VGB85_24900"
     CDS             3893..>4807
                     /locus_tag="VGB85_24900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137355.1"
                     /translation="MAEVEEIASTLENLRARMVDLVVRGLASVAPADLSTLEVLTDSY
                     ERNGLTHITSRLRTMLRAITSDTKAAPAALLSAYTSLHVFERVLSLEVAAGAWQQALS
                     EEEEGDEEETPAPPSAAPGLTLPPAEQKQLAALLDELSRAIEDLVRTGLTSATQATRD
                     KLDVSFKEASRMKLPRLGATLRYVNEEVGRFIAHSDAFSAKRFVFFTHRAWLLAHGLA
                     AALEEKNDRLLARLAGTGARDPIPVRSIDVVTLGMFTRSVQNSCSFEFRLRVVKAEDP
                     TLVGRSLVLGFVFARKSTDIIAEAYLHLP"
BASE COUNT          780 a         1756 c         1514 g          757 t
ORIGIN      
        1 acagggcccg tggaagtcgt ctccgcatca ctcccggtcg tcgcggcgcc ggtctcggag
       61 gatggactgt ccgcgcaggc gaccggcagc gcgagggtca ggacgagggc atggcggcgc
      121 ggcacgtcga gcatgggagc ctcccacgat agacaagccg ccgcgaaccc gcaaacccgg
      181 cgtttgcgcc cgcgaccggg atcactggca gtcgggggcg ggcatgtcgc agggcatgaa
      241 gccgggcggg atgcagccgc tcccggtgtc gaagaccatg ttcggctgtc cttcgggaca
      301 gacgctgacg atcaccggcg gacacgcgac atcgccctgc atgcaggcga ggaattcctg
      361 catggcgaag tcgacgcagg cctcgccgtc ctgctcgatg tgcggggcgc ccatgatcgc
      421 cgtgcagtcg gggttcgcca ggcagccctg ctgcggcagc tgctcgcacg cggtgtcgcc
      481 acacagcggg aagtcgcccg gcacacacgc cttgaacccc ggcggcacgc agccgttcgg
      541 cagccggtag acctccatgc cgggctcctc ctcacagatg tcctggatgt tgctgtcgca
      601 cgccatcatc ggcatgcagg cgataaaggt ctgtccgggc atgcagcccg cgaagtcgaa
      661 ggcctcaccg acgaccgcca tgcagtccgg gtcctgctcg cactgctcct gggtctgcaa
      721 cgcctcgcag ccgtcgaccg gcccggacgt ggtccccgtc ccggtcgcgt cggtgccggt
      781 ccccgtgccg ccggcgctgg agtcgccgct gctcccggtc gggccgtcgc ccgtcggcac
      841 gccggactcg ccaccggtcg tgtccgtggc gccggtgctc gcgccggtgg tcccgccggt
      901 cgtctcggtg tcgcttttgt cggggccgca ggccaggccc gtggagagga ggagcagcat
      961 tgaagtcaag cgcatgcgcc gaggatgccc ggtttcctgc ccgctcgcag gggccttcgc
     1021 cggtacgggc cgcgtatgtc cccgggtcgc gccacgaatg ctcgatcgac tgattggaca
     1081 aaccccatta tcagcgacgc cgcaaaatgc tggcaccagc tgtttcacgg cctttcattc
     1141 gacgcacctt cgccagcgcg cgtagtcgcg ctcgtacgaa ctttcggttc actgtcctac
     1201 atcttcacac ctccttgcct gttcgccgtt gccgaccccc ccagaaccgc gagaaatggt
     1261 ccctgctggc catccagact cgcgcatccc ccgcactgtg gcacacccca tcggatcgcg
     1321 gtgcaagccc cgacgaaata ttgatctgga tcaccgaaat ttttgccagg catcctgcgc
     1381 attgtttcgc ggttatccgc gagatgtgag gcggccggcg agcccggcgg tgactccgct
     1441 tgtgccggtt gtcggagtat tgcgatgttt ccatcggtgc gaccgcgcac aggctgacgt
     1501 gatcttcggc agctttcccg tgttcgtgag gcgaaccggg cggtttcagg gccgccgagg
     1561 cctaacaaca gatagctacg caaggctgca aagctgacac ctcacattgg taaaacgttg
     1621 aacgcgcgct ttgacaaccg ccgggggcag cgcagtctgg ccttacgacg ccccgacccg
     1681 ccgcggggca tccggtcgcc gcccgctcac cggcggcccg ctcagaaccc acaaagctcc
     1741 gattagaggg aactctgcca tgaaccgcca agcctcgacc gccgccgtca tcgcccccaa
     1801 cgcccccgcc gacctcgacg cgctgccgga gctgcgtgcc ctcgaagccg aggtcgcgcc
     1861 ggtggagctg acggagaccg gcgagtcgac cggtttcctc gccccctatt tccgcgacct
     1921 ctccaatgtc gacgtgatgt cccgcgacga ggagctgcag gctgcgatcc gcctgaccaa
     1981 tcttcgcaag tcgtactgga agtcgatcct cagctatcct cctttcatcg acggtatctg
     2041 tgatctcgcc cgcgccgagc tgccggcgga gacctgcccg gtcgaggcgc tcgacgcgat
     2101 gaagaaggcc tcgcgcaacc tgcgtgaccg cgacctgctc gtgcatcaga aggcctacga
     2161 ggccgcctgc gagcagctga cgctcgccat ggccgaggcg gacgtcgacg gcgtcgtctc
     2221 cgaccgcgtg ctcgccgacc tgacctcggt cgaggccggc tgtaacgagg gtctgagcat
     2281 gaaggtcaag ctgccgcgca agggcagcct gccgttcatg agctacgtcg ccgccgcgcg
     2341 ccgcaatcag caggcgcttt ggctcgccaa gaacgcgttc gtcaaggcga acctgcgcct
     2401 ggtcgtcacg atcgcccgcc gcttcaatca cggccgcctg ccgctgcagg acctgatcca
     2461 ggagggcaac atcggcctga tgaaggcggt cgaccgcttc gaccaccgca agggctttcg
     2521 cttctccacc tacgcgagct ggtggatccg tcacgcggtg acccgtgcga tcgccgacaa
     2581 ggcccgcgct gtccgcctgc ccgtccacat gatcgaggcg tacaacaagg tcagcaaggc
     2641 ccgccgcgag ttcgaggccc tgcacggccg ccgcccgacc gacgccgagc tggcgaccgc
     2701 caccggcgtg tccgccgagc gcatccagcg gatgcactac tcgctcgtcg agaaccccgt
     2761 cagcctcgac cagccgctgt ccgaggactc gggcctgacg ctgctcgacg ccgtcgagga
     2821 caccaacctc gccctgccct ccgagctgat ggacaacgag ctgatgatgg gccagctgcg
     2881 cgaggtcttc gagaccctgt cgccgatgga ggccgacatc ctgcgcaagc gcatgggcct
     2941 cgacgacaac caggagatga ccctcaagga gatcggcgcc cgctactcgc tgtcccgcga
     3001 gcgcaaccgc caggtccagg agcaggcgct cgccaagctc cgcggcgagt tcaagcgtcg
     3061 cgcgctgatg taagacccac gcgttcgctg ttcgggcccc cgttcctccg ccaccgcgga
     3121 tggtccgggg gctccggcat tttaaggccc gcgccgccct aatactcgcc gcgcgctgtg
     3181 cctgatccgg atcagggcgg ttttgcccgc ttacaagcca tggggcaggt cggttactct
     3241 cgcgtccgcg gagggttaga gtttccatgg ccacaagacg gatcgtcgca tcgccgcctg
     3301 cccctgttgc agcacccact caaccggcgc cggcaccgac accggcgcct gcgcccgcga
     3361 catcctccgc gaagccccgg cgcaccgtcg gcagccccga gccccggacc ggcaccctcg
     3421 gcaccctgtt caccgagctc gaccaggcga tcggtgacgg gcgcatggag aaggtccgct
     3481 cgatgctccg cgccgaccgc tttcagctgt tcagcgaggt cggcgacgac aaggtcaccg
     3541 ccgtcatcaa gagccagacc aacgcatccc tgttctacgc gtgcagcgtc gacaagggcg
     3601 gccgttacat gtgctgtacg caaaacctcg tccgctgcgg tggcctgaaa ggcaacaagc
     3661 cttgcaagca cctgctggtg atgctcgtcg gcctcgcgca gggccagcag ctcgatccgg
     3721 gcgtcgcttt gcaatgggct cgcgcgacca agaacaaggc cccctcgctc gacaaggact
     3781 cgatgagcgc gaccttcatc cgctacaagg gtgccgaggc cggtgaggtc gactggcgcc
     3841 ccaccgagac gatccccgaa gactactacg cgttttaaaa aggggggagc acgtggccga
     3901 ggtcgaagag atcgccagca ccctcgagaa cctccgcgcg cggatggtcg acctcgtcgt
     3961 gcgtggcctc gcctccgtcg ccccggccga cctctcgacg ctcgaggtcc tcaccgacag
     4021 ctacgagcgc aacggcctca cgcacatcac ctcgcgcctg cgcacgatgc tgcgggcgat
     4081 caccagcgac acgaaggccg cgcccgcggc cctgctgtcg gcctatacca gcctgcacgt
     4141 gttcgagcgc gtgctcagcc tcgaggtcgc cgccggcgcg tggcagcagg cgctcagcga
     4201 agaggaggag ggcgatgagg aggagactcc cgccccgccg agcgccgcgc ccggcctcac
     4261 cctgccgccc gctgaacaaa agcagctcgc cgcgctgctc gacgagctct cgcgcgcgat
     4321 cgaggacctc gtacgcaccg gcctgaccag cgcgacccag gcgacccgcg acaagctcga
     4381 cgtctcgttt aaagaggcct cgcgcatgaa gctgccgcgc ctcggcgcca cgctgcgtta
     4441 cgtcaacgag gaggtcggcc gcttcatcgc ccacagcgac gccttctcgg ccaagcgctt
     4501 cgtgttcttc acccaccgcg cctggctgct cgcgcacggc ctcgccgccg cgctcgagga
     4561 gaagaacgac cgcctgctcg cgcgcctcgc cggcaccggc gcccgcgacc cgatcccggt
     4621 gcggtcgatc gacgtcgtca cgctcggcat gtttacccgc agcgtgcaga acagctgcag
     4681 ctttgagttt cgcctgcgcg tcgtcaaggc cgaggacccg acgctcgtcg gccgctcgct
     4741 cgtgctcggc ttcgtgttcg cgcgcaagtc gacggacatc atcgccgagg cgtacctcca
     4801 cctgccg
//
LOCUS       DASWNJ010001497         3059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_592245, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001497 DASWNJ010000000
VERSION     DASWNJ010001497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592245"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VGB85_24905"
     CDS             <1..472
                     /locus_tag="VGB85_24905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137356.1"
                     /translation="PEPEPEPEPEPIKQAPPPEPEPAKQAPPPEPVKPAKVEKPPAAV
                     VPEPKPAPAKKPEPRDPMDDRPESITKGRLEAKLKSMEARVTSRCRSRAGVPDPRGIT
                     VTVRVVVDTRGEVKATSTGAYAGTPMGQCIEEMLEAIRFNETRSGGSHQHTFRF"
     gene            complement(589..1044)
                     /locus_tag="VGB85_24910"
     CDS             complement(589..1044)
                     /locus_tag="VGB85_24910"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY0137357.1"
                     /translation="MSREASPTWFFALVVVRRGDRFLLVHERKHGQTWYLPAGRVEPG
                     ETIAAAALRETLEETGVPVTLDGVLRIEHNPGTSGVRVRVFFHAHAADDTPAKSTPDE
                     HSLAARWLTVAEMRELPLRGDEVLDIANAVLAGAPVHPLGLLCAEGAPW"
     gene            complement(1047..2105)
                     /locus_tag="VGB85_24915"
     CDS             complement(1047..2105)
                     /locus_tag="VGB85_24915"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEY0137358.1"
                     /translation="MHDPSESTIARALAEFPALVKPVATPLAGGLINRSFAVTAADGE
                     YVLQRVHPVFAPEVHENIAAVTEQLRRRREPSPRLLAARDGRWWADLGDEGIWRVMTR
                     LPGVSFDTIQSTAQARAAAARVGAFHAALGDLEHTFKAVRAGAHDTSAHMHTLAQALS
                     DHPDHRLQPEVAELAAHIVAGYAGLPPPAEVPLRVCHGDLKFNNLLFRGTGPEDQVVS
                     TGIIDLDTVGRMPLHHELGDAWRSWCNRGGEDQTSASFDLEIFAASLDGYLGALSEPL
                     AISERHALVHGVEWISLELSARFAADALNERYFGWDRGRFPAAGEHNLLRARGQWALH
                     LAAIDTREARATLLLSGS"
     gene            complement(2105..>3059)
                     /locus_tag="VGB85_24920"
     CDS             complement(2105..>3059)
                     /locus_tag="VGB85_24920"
                     /inference="COORDINATES: protein motif:HMM:NF013050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137359.1"
                     /translation="ACIPVAWFYSSLWRRAEGEDKERLGALLPLCAAVIAFWAIFHQN
                     GDALTTWAERYTERDMPVAMANVASEVGLAQEVYSEHRVPIKDAAGKVINGPDGEPKT
                     ELRPEPYFSNAPKSEVPPPAPAQCHDAVEDTPEVAQECSKYSVKLISSELFQSVNAFF
                     VVALTPVVVLVFGWLRRRKLEPSTPGKIALGMFITALSTLVMVGAAILTHNGAEKGSA
                     MWLVGAYFVVTIGELCLSPMGLSMVSKLSPPRLTALMMGAWFLSTSIGNKLAGILSGL
                     FDAFEVKAYAFGINFLLALASCGLIVAMLPRLKRVMAKYLN"
BASE COUNT          484 a         1146 c         1033 g          396 t
ORIGIN      
        1 gcccgagccc gagcccgagc ccgagcccga gcccatcaag caggcgccac cgcccgagcc
       61 cgagcccgcc aagcaggccc caccgcccga gcccgtcaag ccggccaagg tcgagaagcc
      121 gcccgcggcc gtcgtccccg agcccaagcc cgcgccggcc aagaagcccg agccgcgcga
      181 cccgatggac gaccgccccg agagcatcac caagggtcgc ctcgaggcca agctcaagtc
      241 gatggaggcg cgcgtcacct cgcgctgccg cagcagggcc ggcgtccccg acccccgcgg
      301 tatcaccgtc accgtccgcg tcgtcgtcga cacccgcggc gaggtcaagg cgacctccac
      361 cggcgcctac gccggcaccc cgatgggcca gtgcatcgag gagatgctcg aggcgatccg
      421 cttcaacgag acccgcagcg gcggcagcca ccagcacacc ttccgcttct gatctcacgc
      481 ggggccggtc ggccctgccg gcagatcgca cattggcccg actgcttcgt ggcgatcgac
      541 gagtgtcacc gccagacggc ggtgcgagcc gggcccaccg tcactcgatc accagggagc
      601 cccctcggcg cacagcagcc cgagcgggtg cacaggtgcc cccgcgagca ccgcgttcgc
      661 gatatcgagg acctcgtcgc cccgcagcgg cagctcgcgc atctcggcca ccgtcagcca
      721 gcgcgccgcg agcgaatgct cgtcgggcgt cgacttcgcg ggcgtatcgt ccgcggcgtg
      781 ggcatgaaag aacacccgca cgcgcacccc cgacgtaccc gggttgtgtt cgatgcgaag
      841 cacgccatcg agcgtcaccg gcacgccggt ctcctcgagc gtctcgcgca gcgccgccgc
      901 ggcgatcgtc tcgcccggct cgacgcgacc cgcgggcagg taccaggtct gtccgtgctt
      961 gcgctcgtgc accaataaaa accggtcgcc gcgccgcacg accaccagcg cgaagaacca
     1021 ggtcggcgac gcctcgcggc tcatcgtcag ctcccggaca gcagcagcgt cgcgcgggcc
     1081 tcgcgggtat cgatcgccgc gaggtgcagg gcccactgcc cgcgcgcgcg cagcaggttg
     1141 tgttcaccgg ccgccgggaa ccggccgcgg tcccagccga agtagcgctc gttgagcgcg
     1201 tccgcggcga agcgggccga cagctcgagc gagatccact cgaccccgtg gaccagcgcg
     1261 tggcgctccg agatcgccag tggttcggac agcgcgccga ggtagccgtc gagcgaggcc
     1321 gcgaagatct cgagatcgaa gctcgccgag gtctggtcct ccccgccgcg gttacaccac
     1381 gagcgccacg cgtccccgag ctcgtggtgc agcggcatgc ggccgaccgt gtcgaggtcg
     1441 atgatccccg tcgacacgac ctggtcctcg ggacctgtcc cgcggaacag gagattattg
     1501 aacttcaggt cgccgtggca cacgcgcagc ggcacctcgg ccggcggcgg caggccggcg
     1561 tagcccgcga cgatgtgcgc cgccagctcg gccacctccg gctgcaggcg gtggtccggg
     1621 tgatccgaca gcgcctgggc cagcgtgtgc atgtgcgccg aggtgtcgtg ggcccccgcg
     1681 cgcaccgcct tgaacgtgtg ctcgaggtcg ccgagcgccg catgaaacgc cccgaccctc
     1741 gccgccgccg cgcgggcctg ggccgtactt tggatcgtgt cgaagctgac gcctggcaag
     1801 cgggtcatca cgcgccagat gccctcgtcg ccgaggtcgg cccaccaccg tccgtcgcgc
     1861 gcggccagca ggcgcgggct cggctcacgg cgacggcgca gctgctccgt caccgcggcg
     1921 atgttctcgt gcacctccgg cgcgaacacc gggtgcacgc gctgcagcac gtactcgccg
     1981 tccgcggccg tgaccgcgaa cgaccggttg atcagcccgc ccgcgagcgg cgtcgcgacg
     2041 ggcttcacga gcgccgggaa ctcggccagc gcgcgcgcga tcgtcgactc ggaggggtcg
     2101 tgcatcagtt gaggtacttg gccatcacgc gcttgaggcg cgggagcatg gcgacgatga
     2161 gcccgcacga cgcgagcgcc agcaggaagt tgatgccgaa cgcgtaggcc ttgacctcga
     2221 aggcgtcgaa cagccccgag aggatgccgg cgagcttgtt gccgatcgac gtcgacagga
     2281 accatgcgcc catcatcagc gccgtcaggc gcggcgggct gagcttggag accatcgaca
     2341 ggcccatcgg gctcaggcaa agctcaccga tcgtgaccac gaagtacgcg ccgaccagcc
     2401 acatcgcgga gcccttctcg gcgccgttgt gggtcaagat cgcggcgccg accatcacca
     2461 gggtcgacag cgccgtgatg aacatgccga gcgcgatctt gccgggcgtc gagggctcga
     2521 gcttgcggcg gcgcagccag ccgaacacca gcaccaccac cggcgtcagc gcgaccacga
     2581 agaacgcgtt gaccgactgg aacaactccg aggagatcag cttgacgctg tacttgctgc
     2641 actcctgcgc gacctccggc gtgtcctcga ccgcgtcgtg acactgcgcc ggcgccggcg
     2701 gcggcacctc ggacttcggc gcatttgaaa aatagggctc gggcctgagc tccgtcttcg
     2761 gctcgccgtc gggtccgttg atcaccttgc ccgccgcgtc cttgatcggc acgcggtgct
     2821 cggagtagac ctcctgggcc agcccgacct cgctggcgac gttggccatc gcgaccggca
     2881 tgtcgcgctc ggtgtagcgc tcggcccagg tcgtcagcgc gtcgccgttt tggtggaaga
     2941 tcgcccagaa cgcgatcacc gcggcgcaca gcggcagcag ggcgccgagg cgctccttgt
     3001 cctcgccctc ggcgcggcgc cacagcgacg agtagaacca cgcgaccggg atgcacgcg
//
LOCUS       DASWNJ010001498         3535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_378279, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001498 DASWNJ010000000
VERSION     DASWNJ010001498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378279"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1312
                     /locus_tag="VGB85_24925"
     CDS             161..1312
                     /locus_tag="VGB85_24925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020055843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0137360.1"
                     /translation="MLFTDEHELLRKSLRTFIEREINPHVDGWEEDKIFPAHEVFKKL
                     GTEGFLGLTKPEEFGGAGLDYSYAIVLAEELGRIPSGGVSLAVGVQTDMCTPALARFG
                     SDELRKEWLTPSIAGDLVGCIGVSEVGSGSDVASLKTTARKDGDDYIINGGKMWITNG
                     IQADWMCCLVNTSDDKAHKNKSLVVIPMDTKGVERARKLHKLGMWSSDTAQIFFDDVR
                     VPQRYRIGPEGAGFMLQMMQFQEERMWGAANSITAMELAISETIAYTRERKAFGKSIL
                     DNQVVHFRLAELTTEVEALRALVYRAAELFLSGKDVTRLASMCKLKAGRLVREVSDSC
                     MQYWGGMGYMWESKIARMFRDSRLASIGGGADEIMLSIICKLDGTLPRG"
     gene            1331..2167
                     /locus_tag="VGB85_24930"
     CDS             1331..2167
                     /locus_tag="VGB85_24930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY0137361.1"
                     /translation="MSQATLPACQSLLLRRDGWRLHVTLNRPEVKNAMSFAMVRELTE
                     VFAAIADDRSLRAVILRGAGGNFCAGGDIKDMAAARNAEPGPDGKDPIAVGNRSFGAM
                     LMRVEAAPQAVIAVLEGAVMGGGFGLCCVSDVAIADEAARFRLPETGLGVPPAQIAPF
                     IVRRVGLTQARRLAVTGGSLDAPQALALGLVHFLHHVSSGTSLDDQVTQVLADIARCG
                     PEAVAVTKRLVLIAAGAHAGTLEAVLDDAAGDFARAARGAESVEGMTAFLQKRRPSWA
                     DA"
     gene            2186..>3535
                     /locus_tag="VGB85_24935"
     CDS             2186..>3535
                     /locus_tag="VGB85_24935"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEY0137362.1"
                     /translation="MSTFNTILIANRGEIACRIIRSAHALGYRCVAVYSQADADAPHV
                     GLADRAVMIGPPPVRESYLNVERLLAAAVAAGADAVHPGYGFLSENAAFAQACLDAGL
                     VFIGPSPAAIDAMGNKAAAKRAMIAAGVPCIPGFQDSSDEAQQDDHLHAEALKVGFPL
                     LIKAAAGGGGRGMRKVTAESELTAAIRSARSEAASAFGSGELIVERAIVDGRHVEIQV
                     FGDTHGNVIHLGERDCSVQRRHQKVVEESPSPAVDAALRERMGAAAVAAARTIDYVGA
                     GTVEFMLDASGEFYFLEMNTRLQVEHPVTESVTGLDLVELQLRVAAGEPLPLTQEQVQ
                     LTGHAIEVRLYAEDPHAGYLPQSGPVHAWQPASGAGVRIDHGIRAGQQISPYYDPMLA
                     KVIAYGRNRDEARRRLVRALEQTVLLGVVHNKQFLADVIAHPVFAAGGATTRFLEQHL
                     "
BASE COUNT          548 a         1343 c         1126 g          518 t
ORIGIN      
        1 ggacgacggc atcatcgacc cgcgtgacac ccggcacgtc ctggcgcagt gcctcgctac
       61 ttgtcgcgag gcggaacttc gcaccctgcg ccccaacacc ttcggcgtcg cccgcctgta
      121 gcttcacctg tcccgaagac cctccgacga aagacccgtc atgctgttca ccgacgagca
      181 cgagctgcta cgaaagagcc tgcgcacctt catcgagcgg gagattaacc cccacgtcga
      241 cggctgggag gaggacaaga tcttccccgc gcacgaggtc ttcaaaaagc tcggcaccga
      301 gggcttcctc ggcctgacca agccggagga gttcggcggc gccggcctcg actactcgta
      361 cgcgatcgtg ctcgccgagg agctcggccg gatccccagc ggcggcgtgt cgctcgcggt
      421 cggcgtgcag accgacatgt gcacgcccgc gctcgcccgc ttcgggtccg acgagctgcg
      481 caaggagtgg ctcacgcctt cgatcgccgg cgacctcgtc ggctgcatcg gcgtcagcga
      541 ggtcggctcg ggctccgacg tcgccagcct caagaccacc gcccgcaagg acggcgacga
      601 ctacatcatc aacggcggca agatgtggat caccaacggg atccaggccg actggatgtg
      661 ctgcctcgtc aataccagcg acgacaaggc ccacaagaac aagtcgctcg tcgtcattcc
      721 gatggacacc aagggcgtcg agcgggccag aaaactgcac aagctcggca tgtggtccag
      781 cgacaccgcg cagatcttct tcgacgacgt gcgcgtgccc cagcgctacc gcatcgggcc
      841 cgagggcgcc ggcttcatgc tgcagatgat gcagttccag gaggagcgca tgtggggcgc
      901 cgccaactcg atcaccgcga tggagctcgc gatctcggag acgatcgcct acacgcgcga
      961 gcgcaaggcc ttcggcaagt cgatcctcga caaccaggtc gtgcactttc gcctcgccga
     1021 gctgaccacc gaggtcgagg ccctgcgcgc cctcgtctac cgcgccgccg agctgttcct
     1081 gtccggcaag gacgtcaccc gcctcgcctc gatgtgcaag ctcaaggccg gccgcctcgt
     1141 ccgcgaggtc tccgactcgt gcatgcaata ctggggcggc atgggctaca tgtgggaatc
     1201 caagatcgcc cgcatgttcc gcgacagccg cctcgcgtcg atcggcggcg gcgccgacga
     1261 gatcatgctc agcatcatct gcaagctcga tggcaccctc ccccgcggct gatcgcctga
     1321 ctttacggac atgtcacaag cgacactccc cgcctgccag tccctcctgc tccgccgcga
     1381 cggctggcgg ctgcacgtca ccttgaaccg cccggaggtc aagaacgcga tgtcgttcgc
     1441 gatggtccgc gagctgaccg aggtcttcgc cgcgatcgcg gacgaccgta gcttgcgcgc
     1501 cgtgatcctg cgcggcgccg gcgggaactt ctgcgccggc ggcgacatca aggacatggc
     1561 cgccgctcgc aacgccgagc ccggccccga cggcaaggac ccgatcgcgg tcggtaatcg
     1621 ttccttcggc gcgatgctga tgcgcgtcga ggccgccccg caggccgtca tcgccgtgct
     1681 cgagggggcc gtcatggggg gaggcttcgg cctgtgctgc gtcagcgacg tcgcgatcgc
     1741 cgacgaggcc gcccgctttc gcctgcccga gaccggcctc ggcgtgcctc cggcccagat
     1801 cgcgccgttc atcgtccgcc gcgtcgggct cacccaggcc cgccgcctcg ccgtcaccgg
     1861 cggcagcctc gacgcccccc aggcgctcgc cctcggcctc gtccactttc ttcatcatgt
     1921 gtcctccggg acaagtctcg acgaccaggt cacgcaggtg ctcgccgaca tcgcccgctg
     1981 cggccccgag gccgtcgccg tcaccaagcg cctcgtgctc atcgccgccg gcgcgcacgc
     2041 gggcaccctg gaggccgtgc tcgacgacgc ggccggggac ttcgcccgcg ccgcgcgcgg
     2101 ggccgagagc gtcgagggca tgaccgcctt ccttcagaag cgccgcccga gctgggccga
     2161 cgcctgacct gagagacata cgaacatgtc gaccttcaac accatcctga tcgccaaccg
     2221 cggcgagatc gcctgccgga tcatccgcag cgcccacgcc ctcggctacc gctgcgtcgc
     2281 cgtctacagc caggccgacg ccgacgcccc gcacgtcggc ctcgccgacc gcgccgtgat
     2341 gatcgggccg ccgccggtgc gcgagtcgta tctcaacgtc gagcgcctgc tcgccgccgc
     2401 cgtcgccgcg ggcgccgacg cggtccaccc cggctacggc tttttgtccg agaacgccgc
     2461 cttcgcgcag gcctgcctcg acgccggcct cgtgttcatc ggccccagcc ccgcggcgat
     2521 cgacgcgatg ggcaacaagg ccgccgccaa gcgggcgatg atcgccgccg gcgtgccctg
     2581 catccctggc tttcaggaca gcagcgacga ggcccagcaa gacgaccatc tccacgccga
     2641 ggcgctcaag gtcggcttcc cgcttctcat caaagccgcg gccggcggcg gcggtcgtgg
     2701 catgcgcaag gtgaccgcgg agtccgagct gaccgccgcg atccggtcgg cgcggtccga
     2761 ggccgcgtcc gcgttcggca gcggcgagct gatcgtcgag cgcgcgatcg tcgacggccg
     2821 ccacgtcgag atccaggtgt tcggcgacac ccacggcaac gtcattcacc tcggcgagcg
     2881 cgactgctcg gtgcagcgtc ggcatcagaa ggtcgtcgag gagtcgccct cccccgccgt
     2941 cgacgccgcg ctgcgcgagc gcatgggcgc cgctgcggtc gccgccgccc gcacgatcga
     3001 ctatgtcggc gccggcaccg tcgagttcat gctcgacgcc agcggcgagt tctacttcct
     3061 ggagatgaac acccgcctgc aggtcgagca cccggtcacc gagtccgtca ccggcctcga
     3121 cctcgtcgag ctgcagctgc gcgtcgccgc cggcgagccg ctgccgctga cgcaggagca
     3181 ggtgcagttg accggccacg cgatcgaggt acgtctatac gccgaggacc cgcacgccgg
     3241 ttacctcccg cagtccggcc cggtgcacgc ctggcagccc gccagcggcg ccggcgtgcg
     3301 catcgaccac ggcatccggg ccggccagca gatctctccg tactacgatc cgatgctcgc
     3361 caaggtgatc gcatatggcc gcaaccgcga cgaggcccgg cgacgcctcg tccgcgcgct
     3421 cgagcagacc gtgctgctcg gcgtcgtgca caacaaacaa ttcctggccg atgtcatcgc
     3481 tcacccggtg ttcgcggccg gcggcgcgac gactcgcttc ctcgagcagc acctc
//
LOCUS       DASWNJ010001499         1874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_663055, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001499 DASWNJ010000000
VERSION     DASWNJ010001499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663055"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VGB85_24940"
     CDS             complement(<1..193)
                     /locus_tag="VGB85_24940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137363.1"
                     /translation="MSGARGGAAYIMIVLTTINFLNYIDQRLLSAVLERIKLEWTLTD
                     AETGLLGSVFVIVYTLTAPI"
     gene            complement(190..801)
                     /locus_tag="VGB85_24945"
     CDS             complement(190..801)
                     /locus_tag="VGB85_24945"
                     /inference="COORDINATES: protein motif:HMM:NF020398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HEY0137364.1"
                     /translation="MVAWVAMHVQAEVALKEWAVVCAALRDGRQTVLLRKGGIREPSA
                     DRSFHVERRTFALLPSYFHAQDSGRERDLVPAVHAELAALRPPADGLLHIDLHAEVTA
                     VWWLDALASVHALAGMHVLSEACVTDRFHYRRPGLWALHLRVTRLREPAALPDRPGYA
                     GCVSWVHLDEPVVGEGEPIGSDDEHARREAALIDRLGPPGASA"
     gene            808..>1874
                     /locus_tag="VGB85_24950"
     CDS             808..>1874
                     /locus_tag="VGB85_24950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137365.1"
                     /translation="MLTGALSQWLQSPHLSQCLLLALAFALGCAHLRGGEGPQGWHYT
                     VEPSAALDRLDIEVCFRDRIPARLVADEDGAADYLLSATAGGRALKIDGETGEIVAGD
                     ARGKCVRYAVDLARMADEVVERHMVRIGGVVATSPHLWLWRPEKLFYESEVTLSFELP
                     ADMGASVPWPRVAADGRRPRHRLSSTAFRWASQVVLGDFHALRFTAAGSEFEVAVMDL
                     PREISDEGLRQWLDVAATTVARLYDGRFPVPHVQVIVVPYPGGGAPVYFGMALRGGGP
                     AVQLLVSSEAKDAQFPGEWVAIHEFLHHGMPFVQHQDAWLSEGFVTYYTEVLTARAGF
                     QDERAAWGALLAGFGRGARSGT"
BASE COUNT          237 a          668 c          681 g          288 t
ORIGIN      
        1 cgatcggcgc cgtcagcgtg tagacgatca cgaacacgct gccgagcagg ccggtctcgg
       61 cgtcggtcag ggtccactcg agcttgatgc gctcgagcac ggcgctgagc agccgctggt
      121 cgatgtagtt gaggaagttg atcgtcgtca ggacgatcat gatgtaggcc gcgccgccgc
      181 gagctccgct catgcgctcg cccccggcgg cccgagccgg tcgatcagcg ccgcctcgcg
      241 ccgggcgtgc tcgtcgtcgc tgccgatcgg ctcgccctcg ccgacgaccg gctcgtcgag
      301 gtgcacccac gacacacaac ccgcgtaacc gggccggtcc ggcagcgccg ccggctcgcg
      361 cagccgcgtg acccgcaggt gcagcgccca cagccccggg cgccggtaat gaaagcggtc
      421 ggtcacgcag gcctcgctga gcacgtgcat gcccgcgagc gcgtgcaccg acgcgagcgc
      481 gtcgagccac cacacggcgg tcacctcggc gtggaggtcg atgtgcagca ggccgtccgc
      541 ggggggccgc agcgccgcga gctcggcgtg cacggcgggc accaggtcgc gctcgcggcc
      601 gctgtcttgc gcgtggaagt agctcggcag cagcgcgaag gtccggcgct cgacgtgaaa
      661 gctgcggtcg gcgctcggct cccggatgcc gcccttgcgc agcagcacgg tctgccggcc
      721 gtctcgcagg gccgcgcaga cgaccgccca ttccttgagc gcgacctcgg cctgcacatg
      781 catggcgacc caggctacca tggcgcggtg ctgacgggcg ccctgtcgca atggctgcaa
      841 tcgccgcatt tgtcgcaatg cctgctgctg gcgctggcgt tcgcgctggg ctgcgcgcac
      901 cttcggggcg gcgagggccc gcagggctgg cactacacgg tcgagccctc ggccgcgctc
      961 gaccgcctcg acatcgaggt gtgctttcgc gaccggatcc ccgcgcggct ggtcgccgac
     1021 gaggacggcg cggcggacta tctgctgtcg gccacggccg gcggccgggc cctgaagatc
     1081 gacggcgaga ccggcgagat cgtggccggc gacgcccggg gcaagtgtgt gcgctacgcg
     1141 gtcgacctcg cgcgcatggc cgacgaggtc gtcgagcggc acatggtgcg catcggcggg
     1201 gtcgtggcca ccagcccaca tctctggctg tggcgcccgg agaagctgtt ttacgagtca
     1261 gaggtcaccc tgagcttcga gctcccggcc gacatgggcg cctccgtgcc gtggccgcgc
     1321 gtggccgcgg acggccgacg gccgcgccat cgcctgtcgt cgaccgcgtt tcgctgggcc
     1381 tcgcaggtcg tgctcggcga cttccatgcg ctgcgcttca ccgcggccgg gtccgagttc
     1441 gaggtcgcgg tgatggatct gccgcgcgag atctccgacg agggcctgcg acagtggctc
     1501 gacgtcgcgg cgacgaccgt cgcgcggctc tatgacggcc gcttccccgt cccgcacgtg
     1561 caggtcatcg tcgtgccgta tccgggcggt ggcgccccgg tgtacttcgg catggccctg
     1621 cgcggcggcg gtccagcggt ccagctgctc gtctccagcg aggccaagga cgcgcaattt
     1681 cctggcgagt gggtcgcgat ccacgaattt ctccatcacg gcatgccctt cgtccagcac
     1741 caggacgcct ggctgtcgga gggcttcgtc acctattaca cggaggtcct gaccgcgcgc
     1801 gcgggctttc aggacgagcg cgcggcctgg ggcgcgttgc tggccggctt tggccgcggt
     1861 gctcgttcgg ggac
//
LOCUS       DASWNJ010001500         6375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_558175, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001500 DASWNJ010000000
VERSION     DASWNJ010001500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558175"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1102
                     /locus_tag="VGB85_24955"
     CDS             107..1102
                     /locus_tag="VGB85_24955"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEY0137366.1"
                     /translation="MAALTRDDVHRAVGSASDRGSFAGQDLTGIDLSTHVMRQADFSR
                     ASLRNADLHGARLDGCDFSSARLDHADLAGASLRNAWFKAASLVDADLRDADLTGAFL
                     GQADLGTAKLRGAWFGGVELSGTELPANWRHITRVQHVPDRPYDRADIIAAVRRDLAD
                     DDPEAALITLVRMLETRDVDALLELLRATPDLTTLPELRAYELPAKLHARICELTTPP
                     LDETAAELARLARHPLATCQTNGLRWTMTQVIRNGGVAPVVLLRQITGLPLHRAKSLA
                     GFASEQPEATQQTLGLAETGDLSSAAALLTFESHDLDFDLAVRTLWCLRESLRPR"
     gene            1249..1668
                     /locus_tag="VGB85_24960"
     CDS             1249..1668
                     /locus_tag="VGB85_24960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137367.1"
                     /translation="MSSEEIVGTHRVRVDGDVVLTRYIGVPEQAHVEAIHQRFDRVLA
                     EHGRLFVINDMRRTGIPSTETRRWIAEWGRHHPVAGLINVGASLPIRMVQGLLFRAAG
                     LLGRPSPIVAINCITEAEALAIIDDLRRKLALHPVAK"
     gene            complement(1669..2751)
                     /locus_tag="VGB85_24965"
     CDS             complement(1669..2751)
                     /locus_tag="VGB85_24965"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEY0137368.1"
                     /translation="MTELLYTPTKLADLDLKNRVVMAPMTRSRAIGNVPDALTATYYG
                     QRAEAGLIVTEGTAPSANGLGYARIPGLYDAAHVAGWRATTEAVHAEGAKIFVQLMHS
                     GRVGHPNNLPPGGRIVAPSAIAAPGTMYTDQAGPQPHPVPAEMSEAEIDQAIEEFVAS
                     AGLAIEAGFDGVELHGANGYLIDQFLNTASNQRSDGWGGSVEGRVRFALEVARRVASR
                     IGAGRVGMRLSPYGVFGGMVSDPDIDAVYQRLVEGLSDLGIAYVHLVDHSSMGAPPVP
                     EALTRALRAAFRGRTILSGGYDAARAEADLVAGRGDLIAFGRPFIANPRLVTRMRAGL
                     PLAQPDPNTLYTPGERGYTDYPLDPA"
     gene            complement(2864..3310)
                     /locus_tag="VGB85_24970"
     CDS             complement(2864..3310)
                     /locus_tag="VGB85_24970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137369.1"
                     /translation="MIELLAGLKQVIARRRCEVMIEGLPAQVHSRADLRFEVVLRAGP
                     CEIAVRELRVSLEEERLIHLDPGCGEYGFWSTAVRTVLPLPRLHLSPRGSARVPVCLP
                     LPELAPSQLLRRYRLLVVADVPGLNPQTSAIVELVEDASQRPMPPL"
     gene            complement(3541..3918)
                     /locus_tag="VGB85_24975"
     CDS             complement(3541..3918)
                     /locus_tag="VGB85_24975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137370.1"
                     /translation="MFSNTMCKSFLAAAIMIVAACDEMAEEQALALDEQAEAAPQDRQ
                     KTADAPEELAPDAAPAWSELDITDAPQGVEGPDGKTCCADCGDGWAGWYNLGGADNCN
                     GRAAAFCGQNGWYFINAEWRYAC"
     gene            complement(4157..5386)
                     /locus_tag="VGB85_24980"
     CDS             complement(4157..5386)
                     /locus_tag="VGB85_24980"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY0137371.1"
                     /translation="MDRRKFLGIASYAGLSVAAFDGFGRPARAGSRAGYTPLSQANRR
                     PLFITVNARGGWDPTSLCDPKGAASDVEVDAMNWSFRARDIGKTASGIPYAPLGSAER
                     PDYFQRFFEKHDRQLLVINGVHVGTRGHDDGAAHMWSGRLAGDHPSFTALVAAMHGGE
                     APMGYLSFGGHDETLGECARTRSGDSRRLGGAFNPDRAEPEDETTGFALGADGSVIFA
                     SRTGAEELDLLRRYLPELDNGDNPLIRQIQVAMAAYRAGITAAVNLELDGFDTHEDHD
                     AGQIPRLCQLLAGLDFIGEEAARQDLGDDYVVVASSEFGRSPGYNASNGKDHWPVTSV
                     LAMGKGIRGGRVIGATTERHEALPVDPQTLAPSARGVIIEPAHIHHALRRLAGIDGHP
                     LAQRYALDDADDMNLFG"
     gene            complement(5447..>6375)
                     /locus_tag="VGB85_24985"
     CDS             complement(5447..>6375)
                     /locus_tag="VGB85_24985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137372.1"
                     /translation="THRGGVAGEPGPAANDLLAWRTTMKIGATIVKEEIPYWEPKLLK
                     NEWMRIFPNSDGTLPSWSDARFAYMQRVGGEPFASTKLDGDPAKIKALRERLLALPGW
                     IKTLWLTDRHEPEGDVSGSAYKAHFKAYYDMIKGLPSDIRSRVKAGPVLTRQATENSG
                     GLGTYATYDPGVGDFFGVDMYGNSWGSPATAAATSYVDPKTFLAKVKAYSTGTRPKLF
                     PEIGYIGLPFDKDGSKRAAWIAGITEELKTWSNFVGYIWWNANGKTGSSISGLGTKRY
                     FQLDRRHTGTDGSFVTLSPALPLTKLNGYKGI"
BASE COUNT          944 a         2306 c         2132 g          993 t
ORIGIN      
        1 cgcctggctc ggcttctgcg tctacacctg gttcgccccc ggcctgttcc gcaagctgct
       61 cgacagggag ttcaagaccg tcgagctccg ccgggatttt taaaagatgg ccgcgctgac
      121 ccgcgacgac gtccatcgcg cggtgggatc tgcgagcgac cgcggctcct tcgccggcca
      181 ggatctgacc ggcatcgacc tctccaccca cgtgatgcgc caggccgact tctcgcgcgc
      241 ctcgctgcgc aacgccgacc tccacggcgc ccgcctcgac ggctgcgatt tttccagtgc
      301 caggctcgac cacgccgacc tcgccggcgc ctcgctgcgc aacgcatggt tcaaggccgc
      361 gagtctcgtc gacgccgacc tgcgcgacgc cgacctgacc ggcgccttcc tcggccaggc
      421 cgacctcggc accgccaaac tgcgcggcgc atggttcggc ggcgtcgagc tcagcggcac
      481 cgagctcccc gccaactggc gacatatcac gcgcgtccag cacgtcccgg accgccccta
      541 cgatcgcgct gacatcatcg ccgccgtccg ccgcgacctc gccgacgacg acccagaggc
      601 cgccctgatc acgctcgtgc gcatgctcga gaccagggac gtcgacgcct tgctcgagct
      661 cctgcgcgcc acccccgacc tgacgaccct ccccgaacta cgcgcatatg aattgcccgc
      721 caagctacac gcgcggatct gcgagctgac cacgcccccg ctcgacgaga ccgccgccga
      781 gctggcccgc ctcgcccgcc acccgctcgc cacctgccaa acgaacggcc tgcgctggac
      841 gatgacgcag gtgatccgca acggcggcgt cgcgccggtg gtcctgctgc gccagatcac
      901 cggcctcccc ctccatcgcg ccaagagcct cgccggcttc gccagcgagc agcccgaggc
      961 cacccagcag accctcggcc tcgccgagac cggcgacctg agctccgccg ccgccctcct
     1021 gacctttgag agtcacgacc tcgacttcga cctcgcggtc cgcaccctgt ggtgcctgcg
     1081 cgagtccctg cggccccgct gagccggccg aaggtcagcc aacgagcaag tgccgaccgc
     1141 tgcgaccgtc cccgatccat ccgtgcgtgt tcgcgagcaa cacgagcgcc gaccctctgc
     1201 aacggtgaga cgcgaccttc tggcgcgccc gcggtagttt gcgcgctgat gtcgagcgag
     1261 gagatcgtcg ggacccaccg cgtgcgcgtc gatggcgacg tggtgctgac gcgctacatc
     1321 ggcgtgcccg agcaggcgca cgtcgaggcg atccaccagc gcttcgatcg cgtgctcgcc
     1381 gagcacggcc gcctgttcgt catcaacgac atgcggcgca ccggcatccc ctcgaccgag
     1441 acccggcggt ggatcgccga gtgggggcgg catcatcctg tcgccggcct gatcaacgtc
     1501 ggcgcgagcc tgccgatccg catggtccag ggcctcctgt tccgcgcggc cgggctgctc
     1561 gggcgcccgt ctccgatcgt tgcgatcaac tgcatcaccg aggccgaggc gctcgccatc
     1621 atcgacgacc tgcgccgcaa gctcgcgctc cacccggtgg cgaagtgatc acgcggggtc
     1681 gagcgggtag tccgtgtagc cgcgctcgcc gggcgtatac aacgtgttcg ggtcgggttg
     1741 cgcgagcggc agcccggccc gcatgcgcgt gaccagccgc gggttggcga tgaacggccg
     1801 gccaaaagcg atgaggtcac cgcggcccgc cacgaggtcg gcctcggccc gcgcggcgtc
     1861 gtatcctccg gacaggatcg tgcggccgcg aaacgccgcg cgcaacgccc gggtcagcgc
     1921 ctccggcacc ggcggcgcgc ccatgctgct gtggtcgacg aggtgcacat aagcgatgcc
     1981 gaggtccgac agcccctcga ccaggcgctg atataccgcg tcgatgtcgg ggtcgctcac
     2041 catcccgccg aacacgccat acggcgacag gcgcatgccg acgcgcccgg ccccgatgcg
     2101 cgacgcgacg cgacgcgcga cctcgagggc gaagcgcacg cgaccctcga ccgacccgcc
     2161 ccagccgtcg ctgcgctggt tcgacgccgt attgaggaat tggtcgatga gataaccgtt
     2221 ggcgccgtgc agctcgaccc cgtcgaagcc ggcctcgatc gccaagcctg ccgacgcgac
     2281 gaactcttcg atcgcctgat cgatctcggc ctcgctcatc tcggcgggca ccggatgcgg
     2341 ctgcggtccc gcctgatcgg tgtacatcgt cccgggcgca gcgatcgcgg acggcgcgac
     2401 gatgcgacca cccggcggca ggttgttcgg gtggccgacg cgaccgctat gcatcagctg
     2461 cacgaagatc ttcgcgccct cggcgtgcac cgcctcggtc gtcgcacgcc agccggccac
     2521 atgcgcagcg tcgtacaggc cggggatccg cgcgtaacca aggccgttcg cggacggcgc
     2581 ggtgccctcg gtgacgatca ggccggcctc ggcgcgctgg ccgtagtagg tcgccgtcag
     2641 cgcgtccggc acgtttccga tcgcccggct gcgggtcatc ggcgccatca cgacgcggtt
     2701 cttgaggtcg aggtcggcga gcttggtcgg ggtgtacaaa agttcggtca tcgcggcgct
     2761 ccaccaacgg tgtaagcccg ccccccccga ccgggtagcc ggggtccagg aacacactgt
     2821 tgcacccgac gcgccgaccg acgtgctccg agcgacgccg agctcagagc ggcggcatgg
     2881 gccgctgtga cgcgtcctcc acgagctcga cgatggcgct ggtctgcggg ttgaggccgg
     2941 gcacatcggc gaccacgagc aggcgatagc ggcgcagcag ctgcgacggc gccagctccg
     3001 gcaacggcag gcacaccggc acgcgcgcgc tcccgcgcgg cgacagatgc aagcgcggca
     3061 gcggcaggac ggtgcgcacg gcggtgctcc aaaatccgta ttcgccgcag ccgggatcga
     3121 ggtggatgag ccgctcctcc tcgagcgaca cacgaagctc gcggaccgcg atctcgcagg
     3181 gccctgcgcg taacacgacc tcgaaccgca ggtccgcccg cgagtgcacc tgcgccggca
     3241 gcccctcgat catcacctca caccgtcgcc gtgcgatcac ctgcttcaaa ccggcgagga
     3301 gttcgatcat acggccaagc tatcaccgac gatgtacctg tccaccgcat atgaatgatt
     3361 ttatcacgga atgttgatag gagaatcaaa tttctactgt cacatggcgc gcttcgtcca
     3421 gctttcatgg tcgcggcgtc gccggcgcgg cgtgacgcca accagcgcgc tggggggaag
     3481 tgcgccgccg gtcgacccgc ccccggaccg caggaacggg gacgggccgc cgcagggggc
     3541 tcagcaggcg tagcgccact cggcgttgat gaagtaccag ccgttctgcc cgcagaaggc
     3601 cgccgcccgg ccgttgcagt tgtcggcgcc gccgaggttg taccagccgg cccagccgtc
     3661 gccgcagtcg gcgcagcagg tcttgccgtc cgggccctcg acgccctgcg gcgcatcggt
     3721 gatgtcgagc tcggaccacg cgggcgcggc gtccggcgcg agctcctccg gcgcgtcggc
     3781 ggtcttctgg cggtcctgcg gggcggcctc ggcctgctcg tcgagcgcga gcgcctgctc
     3841 ctcggccatc tcgtcgcacg cggcgacgat catgatggcg gcggcgagga acgacttgca
     3901 catggtgttg ctgaacatgg gagaaccttc cgacacgggt agcagcgggc gcggtcggtc
     3961 gcgctcgccg gggacgatat gtcccgctca cggtgaccgg cgatgagcgc cggatcgcgg
     4021 atgcacagcg catccacgat ccctacgtgc ccggtcatcg ccgccgcgat cagtcgctcg
     4081 atacatttct cgagcgcccg cgacgctcaa tccccagcgt gtggtttgtc ggtttctccc
     4141 ccttatacat aaccattcaa ccgaacaggt tcatgtcgtc ggcgtcgtcg agcgcgtacc
     4201 gctgcgcgag cgggtggccg tcgatgccgg cgaggcggcg cagggcgtgg tggatgtgcg
     4261 cgggctcgat gatgacgccg cgggcgctcg gcgccagcgt ctgcgggtcg accggcagcg
     4321 cctcgtggcg ctccgtcgtc gcgccgatca cccggccgcc gcggatgccc ttgcccatcg
     4381 ccagcacgct ggtgaccggc cagtgatcct tgccgttgct ggcgttgtag ccggggctcc
     4441 gcccaaattc ggagctggcg acgaccacgt aatcgtcgcc gaggtcctgc cgcgccgcct
     4501 cctcgccgat gaagtcgagg ccggccagca gctggcacag gcgcgggatc tgcccggcgt
     4561 cgtggtcctc gtgggtatcg aagccgtcga gctcgaggtt gaccgccgcc gtgatccccg
     4621 cgcgatacgc cgccatagcg acctggatct ggcgaatgag cgggttatca ccgttgtcga
     4681 gctcgggcag gtatcgtcgc aggaggtcga gctcctcggc gccggtgcgc gaggcgaaga
     4741 ttacgctgcc gtcagcgccg agcgcgaagc ccgtcgtctc gtcctccggc tccgcgcggt
     4801 cggggttgaa cgcgccgccg agccggcggc tgtcgccgct gcgcgtgcgc gcgcactctc
     4861 ccagcgtctc gtcgtggccg ccgaagctga ggtatcccat cggcgcctcg ccgccgtgca
     4921 tcgcggcgac cagcgcggtg aagctcggat ggtcgccagc gagccggccc gaccacatat
     4981 gcgccgcgcc gtcgtcgtgc ccgcgcgtgc cgacgtgcac gccgttgatc acgagcagct
     5041 gccggtcgtg cttctcgaag aagcgctgaa agtagtccgg tcgctcggcg ctgccgagcg
     5101 gcgcgtaggg gatcccgctc gccgtcttgc cgatgtcgcg ggcgcgaaac gaccagttca
     5161 tcgcgtcgac ctcgacatcg ctggccgccc ccttggggtc acacagcgag gtcgggtccc
     5221 agccgccgcg ggcgttgacc gtgatgaaca gcgggcgcct gtttgcctgc gagagcggcg
     5281 tgtagccagc ccgcgatccg gcgcgggccg gccggccaaa gccgtcgaag gcggcgaccg
     5341 agagaccggc gtacgaggcg ataccgagaa atttgcgacg atccatggtg cagctccttg
     5401 ttcttgtaag tgcgagagat ccgggcggat gtctgcggcc aggccgtcag atccccttgt
     5461 agccattgag cttggtgagc ggcagggcgg ggctgagcgt cacgaaggag ccatccgtgc
     5521 ccgtgtgccg gcgatcgagc tgaaagtacc gcttggtccc cagcccggag atgctgctgc
     5581 ccgtcttgcc gttcgcgttc caccagatgt atcccacgaa gttggaccag gtcttgagct
     5641 cctcggtaat ccctgcgatc cacgcggcgc gcttgctgcc gtccttgtcg aacggcaatc
     5701 cgatgtagcc gatctccggg aacagcttcg ggcgggtgcc ggtgctgtac gccttgacct
     5761 tggcgaggaa ggtcttcggg tcgacatacg aggtcgccgc cgcggtcgcg ggcgagcccc
     5821 atgaattccc gtacatgtcg acgccgaaga agtcgccgac gcccgggtcg taggtcgcgt
     5881 aggtgccgag gccgcccgag ttctcggtcg cttgccgggt cagcaccggg ccggccttga
     5941 cgcggctgcg gatgtccgac gggaggccct tgatcatatc gtagtaggcc ttgaagtggg
     6001 ccttgtacgc cgacccggag acatcgccct cgggctcgtg ccggtcggtc agccacagcg
     6061 tcttgatcca gcccggcaac gcgagcaagc gctcgcgcag cgccttgatc ttggcggggt
     6121 caccgtcgag cttggtcgag gcgaacggct ccccgccgac gcgctgcatg tatgcgaagc
     6181 gcgcatcgga ccacgacggc agcgtcccgt ccgagttggg gaagatccgc atccactcgt
     6241 tcttcagcag cttgggctcc cagtagggga tctcttcctt gacgatcgtc gcgccgattt
     6301 tcatcgtagt tctccaggca agaaggtcgt tcgcggccgg ccccggctcg cccgcgacac
     6361 cgccccgatg ggtcg
//
LOCUS       DASWNJ010001501         2059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_274945, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001501 DASWNJ010000000
VERSION     DASWNJ010001501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274945"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1865)
                     /locus_tag="VGB85_24990"
     CDS             complement(156..1865)
                     /locus_tag="VGB85_24990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137373.1"
                     /translation="MLRLLFASSCLALCGCGLGVLDELAKVPVCDSQATDPECEALFG
                     SSTGGAGVPTTTAMTDATSGGSGVHTVTGDTTLADSTAAADGAGTGEPADPAPTIDLF
                     TAEPDQLSEAGQSRLQLHASPDVVAVRLYQNGEKLADLTPADFPYTYEALSAKYNGDP
                     HIFKVEARDAAGQTATDHLELKVLLPASGTERCSFTDEGMGAASSAITAVVYGKDAIV
                     AAGWRDAGKGQRMTVWKLDKASCDPIPGWPKTIADWTMDPALAMQASQAVGVAIDASG
                     YIAVAANLWPGGKPQMYAALLTPQGSRIWERPGKLGEQAAAIAAGDGHRVFVTGSVRT
                     SDNPVLTDARTWGLNFDNESLTVWSSDLAAPFTPEEADPDIDNKWSERGRAVLVWNGE
                     VFVACEREFMDDQKEVFTRTSIARYQPFGGLIGPPRTSPGDFLSHEAMNALAICGTDL
                     IAGGWTRDKAPGASPVPLMRWIEPDGKSSAGHSELLPSTRTFGVGCDREGKIVSGGTS
                     TAGALNTQVFASEDPKNPPILYDAGVTGDDATLTLACDPDEGFCAGGGFRSPFAYLRV
                     YHP"
BASE COUNT          309 a          686 c          717 g          347 t
ORIGIN      
        1 cactcaagaa tccgcttcgc ctcctcgccg agggggtcga acacgggttc atcggatcga
       61 tctgcgagga gagctatgac ccgttcttca gcgaagccgt cgacgaagtg gtcgcagttt
      121 gcgaaaactt cgtgccgccg ggttagcggc agacctcacg ggtgatacac acgcaggtac
      181 gcgaacggtg agcggaatcc gccgccggcg cagaagccct catccgggtc gcacgcgagc
      241 gtcaaggtcg cgtcgtcgcc cgtgaccccg gcgtcgtaga ggatgggcgg gttcttggga
      301 tcttcggagg cgaagacctg ggtattgagc gcgcccgcgg tgctcgtgcc gccgctgacg
      361 atcttgccct cgcggtcgca gccgacgccg aaggtccgcg tcgagggcaa caactcgctg
      421 tggcccgcgc tcgacttccc gtccggctcg atccagcgca tgagcggcac gggcgaggcg
      481 cccggcgcct tgtcgcgggt ccagcctccc gcgatgagat ccgtcccgca gatggcgagg
      541 gcgttcatcg cctcatgcga tagaaagtcg cccggagagg ttcggggcgg gccgatcagg
      601 ccgccgaagg gttgatagcg ggcgatggag gtccgggtga agacctcttt ctgatcgtcc
      661 atgaactctc gttcgcaagc gacaaacacc tcaccgttcc agacgagcac ggcccggccc
      721 cgctcgctcc acttattatc gatatccggg tcggcctcct ctggcgtgaa cggggccgcg
      781 aggtccgatg accagacggt caggctttca ttgtcgaagt tgaggcccca ggtcctcgcg
      841 tcggtgagga cagggttgtc gctggtgcgc accgagcctg tgacgaacac gcgatgcccg
      901 tccccggcgg cgatcgccgc ggcttgctcg ccgagcttgc cgggacgctc ccagatccgt
      961 gagccttgcg gggtcagcag cgccgcgtac atctgcggct taccgccggg ccagaggttc
     1021 gccgcgacgg cgatgtagcc cgatgcatcg atcgccacgc cgaccgcctg cgaggcttgc
     1081 atcgccaggg cagggtccat ggtccagtcg gcgatcgtct tcggccagcc cgggataggg
     1141 tcgcagctcg ccttgtcgag cttccagacc gtcattcgct ggcccttgcc cgcgtcgcgc
     1201 cagccggccg cgacgatcgc gtcctttcca taaaccaccg ccgtgatcgc gctcgacgcg
     1261 gcgcccatgc cctcgtccgt gaacgagcag cgctcggtcc ccgacgccgg cagcagcacc
     1321 ttgagctcga ggtggtcggt ggccgtttgt cccgcggcgt cgcgggcctc gaccttgaag
     1381 atgtgcgggt cgccgttgta tttggccgag agcgcctcgt aggtgtacgg gaagtcggcg
     1441 ggggtgaggt cggcgagctt ctcgccgttc tggtagagcc gcaccgcgac gacatcgggg
     1501 ctggcatgca gctgcaagcg cgactggccg gcctcgctga gctggtcggg ctcggccgtg
     1561 aacaggtcga tcgtcggcgc aggatcggca ggctcgccgg tgccagcccc gtccgccgcg
     1621 gccgtgctgt cggccagtgt ggtgtctccg gtgacggtat ggacgccgga cccgccggac
     1681 gtcgcgtcgg tcatcgcggt cgtggtcgga acgcccgcgc cgccggtcga gctgccaaac
     1741 agcgcctcgc actcagggtc ggttgcctgg ctgtcgcaga cgggcacctt ggcgagctcg
     1801 tcgagcacac ccaacccgca gccgcacaag gcgaggcatg aagatgcgaa caggaggcgg
     1861 agcatgccca tgagtagcaa aatcgcgccg cgcggaatag cgcgatattt cccgtaatag
     1921 cgccgcccgc tacagtgctg gcgatgcgct ggctcctcgc gctgctggtg ttggtcgcct
     1981 gtggccggcc gggcgggatc ccggtgctgg tgatcccggg caagatgccc ggcggcgccg
     2041 actatgccga cccgatgca
//
LOCUS       DASWNJ010001502         1561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_310282, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001502 DASWNJ010000000
VERSION     DASWNJ010001502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310282"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1561
                     /locus_tag="VGB85_24995"
     CDS             <1..>1561
                     /locus_tag="VGB85_24995"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0137374.1"
                     /translation="LAAELIAAGRAPDDARREALQRFGNLEAVRARCHDISSHRATTM
                     RRHELLSTVWQDVVYAARGMRRARGFSLVVLLTLALGIGASTAMFSVVRGVLLRPLPF
                     PDAERVVRLWPGNPKVAVEGRVVSARELEDWERELRGFEAVGAFGVIPMGHVYGEGGA
                     EPAYARTSYVSRGFFPALGTPARLGRTLRAEEHVDGANRVIVVSDAFWRAQLGADPTV
                     VGRVVRLDGKPYTVVGVMGPDFAFPDPGISVWVPSSVMDPDDAAWRGRERRWLSPVGR
                     LRPGVTPAQGREEVEALLQRLATAYPQTNAGWTSAVVEDVRDSIVGKVRQGLLVLLGA
                     VGLVLLVVCANVANLLLVRATGRGREMAVRAAIGAGRGRVVRLLLTESLLLALAGGAL
                     GVALAWWGVRTLVALSGHFLPRATDVRLDLGVLGFAVGVTLLTALVSGLWPALRASSP
                     RLSNALREGARGSSGGAASHRARAALVGAEVALAVVLVASAGLMLRSFERLTSVDPGF
                     RPEGALLARFHL"
BASE COUNT          139 a          577 c          621 g          224 t
ORIGIN      
        1 ctggccgcgg agctgatcgc ggccggccgc gcgcccgacg acgcacgccg cgaggcgctc
       61 cagcgcttcg gcaacctcga ggcggtgcgc gcgcgctgcc acgacatctc ctcccaccgc
      121 gccaccacga tgcgccgtca cgagctgctc tccaccgtct ggcaggacgt cgtgtacgcc
      181 gcccgcggca tgcggcgcgc gcgcggcttc tcgctcgtcg tcctcctcac cctcgctctc
      241 gggatcggcg cgtcgacggc gatgttctcc gtcgtccgcg gggtgctcct gcgcccgctc
      301 ccctttccgg acgccgagcg cgtcgtgcgg ctctggcccg gcaacccgaa ggtcgcggtc
      361 gagggtcgcg tcgtcagcgc cagggagctc gaggactggg agcgcgagct gcgcggcttc
      421 gaggcggtcg gcgcgttcgg cgtgatcccc atggggcacg tgtacggcga ggggggcgcg
      481 gagccggcgt acgcgcggac gtcgtacgtc tcgcgcggct tcttccccgc gctcggcacg
      541 ccggcgcgcc tcgggcggac gctgcgcgcc gaggagcacg tcgacggcgc gaaccgcgtc
      601 atcgtggtga gcgacgcgtt ctggcgcgcg cagctcggcg ccgacccgac ggtcgtcggc
      661 cgcgtggtcc ggctggacgg caagccgtac accgtcgtcg gcgtgatggg gccggacttc
      721 gccttcccgg atccgggcat ctccgtgtgg gtcccgagca gcgtcatgga cccggacgac
      781 gccgcgtggc gcgggcgcga gcggcgctgg ctgagccccg tggggcggct gcgcccgggg
      841 gtcacgccgg cgcaggggcg cgaggaggtc gaggcgctgc tgcagcggct cgcgacggcg
      901 tatccgcaga ccaacgccgg ctggacgtcg gcggtcgtcg aggacgtgcg cgactcgatc
      961 gtcggcaagg tgcggcaggg gctgctggtg ctgctcggcg cggtggggct cgtgctgctc
     1021 gtcgtgtgcg cgaacgtggc gaacctgctc ctcgtgcgcg ccaccgggcg cgggcgcgag
     1081 atggcggtcc gcgccgccat cggggcgggg cgcgggcgcg tcgtgcggct gctgctcacc
     1141 gagagcctgc tgctcgcgct cgccggcggc gcgctcggcg tggcactcgc gtggtggggc
     1201 gtgcggacgc tcgtcgcgct gagcggccac ttcctccccc gcgccacgga cgtgcggctc
     1261 gacctcggcg tgctcggctt cgcggtcggc gtcacgctgc tcaccgcgct cgtctccggg
     1321 ctctggccgg cgctgcgcgc gtcgtccccg cggctgtcaa atgcgctgcg cgagggcgcg
     1381 cgcgggagct ccggcggggc cgcgtcgcac cgcgcccgcg cggcgctcgt cggggccgag
     1441 gtcgcgctcg cggtcgtgct ggtggcgagc gcggggctca tgctccgcag cttcgagcgg
     1501 ctcacgagcg tggaccccgg cttccgcccg gagggggcgc tgctcgcgcg cttccacctg
     1561 g
//
LOCUS       DASWNJ010001503         1558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_97127, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001503 DASWNJ010000000
VERSION     DASWNJ010001503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97127"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VGB85_25000"
     CDS             complement(<1..605)
                     /locus_tag="VGB85_25000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137375.1"
                     /translation="MKKPEQQWETARFIAVRLPEPPPERAQEPGVQAFELRARDGGLS
                     ARACFSLLRQEVDAAAADDDLVFAARAWLAARGGRSLNLTDQTGAGIAGGPFTLAELS
                     DPPAGPAARVVTLCPSNAEIVGALGGFDRVIACEDSSDWPPEVAQRERLGPDLGPDLD
                     RVAALAPALALSSLSVPGMERVVTGLRVRGVPQRVLAPRSL"
     gene            804..>1558
                     /locus_tag="VGB85_25005"
     CDS             804..>1558
                     /locus_tag="VGB85_25005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0137376.1"
                     /translation="MEISIKDAAELLGKSERTVRHMAQTGRLPARRIGSRWLINRDDL
                     LARKAAGLGNDSDEPDGSDGSEAPDGNEVDGADSETAELAADDSSDSSPRRPSRQGFT
                     VNFAPGHSVALPPGAVTSVRELDSFHIATDILAELGELRRAVKDAAPLRDAETHLREF
                     LQLLADGCHADLATRRDRFQAAQSQACAALTDLIHFNVSRAEPDLGPLGERVERDLIG
                     SLRMLLEDTQRHLSVRGRVLDLAERAVARVLSRL"
BASE COUNT          232 a          619 c          519 g          188 t
ORIGIN      
        1 tcgagcgacc gcggcgcgag cactcgctga ggcacgcccc gcacgcgcag gccggtgacg
       61 acgcgctcca tcccgggcac gctcagcgag ctcagcgcca gggccggggc cagcgcggcc
      121 acgcgatcga ggtcgggccc gaggtccggc ccgaggcgtt cgcgctgcgc cacctccggc
      181 ggccagtccg atgagtcctc gcaggcgatc acgcggtcga agccgccgag cgccccgacg
      241 atctccgcgt tcgacgggca cagcgtgacg acgcgagcgg ccggccccgc gggaggatcc
      301 gagagctccg ccagcgtgaa tggcccgccg gcgatcccag cgcctgtctg atcggtcagg
      361 ttcaatgaac gcccgccccg ggccgccagc caggcccgcg cggcgaacac caggtcgtcg
      421 tcggccgccg ccgcgtcgac ctcctgccgc agcagcgaga agcaggcccg cgcggacagc
      481 cctccgtcac gcgcgcgcag ctcgaaggcc tgcacccccg gctcctgcgc acgctcgggc
      541 ggcggttccg gtagccgcac cgcgataaag cgcgccgtct cccactgctg ctccggcttc
      601 ttcaccggcc gaccctcgcc tccgcgccag ggggcgtcaa gcgggcccga cctgaaccca
      661 tggccaggat cgcgccgcgt ccggcaatgg cgatggccga gttcggcaag aattattgcc
      721 gtcgatgctg cgcgtgatcc gacttcggct aggatgtgcg cggccatccc ccggccgatc
      781 gcccacccga cgaggaaagc tccatggaga tctccatcaa agacgccgcc gagctgctcg
      841 gcaagagcga gcgaaccgtg cgccacatgg cccagaccgg tcgcctgccc gctcgccgca
      901 tcggctcgcg ctggctcatc aaccgtgacg acctgctcgc ccgcaaggcc gccggcctcg
      961 gcaacgacag cgacgagcca gacggcagtg acggcagtga ggcacccgat ggcaacgaag
     1021 tcgacggcgc ggacagcgag acggccgagc tcgcggccga cgactccagc gacagctccc
     1081 cgcgcaggcc gtcacgccag ggctttaccg tgaatttcgc gcccggccac agcgtcgcgc
     1141 tgccgcccgg cgccgtcacc tcggtgcgcg agctcgacag cttccacatc gccaccgaca
     1201 tcctcgccga gctcggcgag ctaaggcgcg cggtcaagga cgcggccccg ctgcgcgacg
     1261 ccgagaccca cctgcgcgag ttcctgcagt tgctcgccga cggttgccac gccgacctgg
     1321 cgacccgccg cgaccgcttt caggccgccc agtcgcaggc ctgcgccgcc ctgaccgacc
     1381 tgatccactt caatgtctcg cgcgccgagc ccgacctcgg cccgctcggt gagcgcgtcg
     1441 agcgggacct catcggttcg ctgcgcatgc tgctcgagga cacccagcgc cacctcagcg
     1501 tgcgcggccg ggtgctcgac ctcgccgagc gagcggtggc gcgggtgctg tcgcgcct
//
LOCUS       DASWNJ010001504         2089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_663153, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001504 DASWNJ010000000
VERSION     DASWNJ010001504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663153"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..1530
                     /locus_tag="VGB85_25010"
     CDS             49..1530
                     /locus_tag="VGB85_25010"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEY0137377.1"
                     /translation="MLTIDWTSQDPTAAALQTLAVAIPGRDNAPAVEQLFGAATAQAA
                     TAARFRGRPGEQFRFTREQAGALQTVVLLGVGADPLAPGRWRTLAHAAVRAASEVGSE
                     HLVLDLRQVAPLAAVITAPQLGNLLCQGLELGTYRYDRYIAEARRKPSTVKRVSVVGV
                     DPAGNEGVDRGQTIASAVARARDLVNGPANEVTPTYLARFAADVVTRLGGAADVKLTV
                     LEREDCEKLGMGSYLAVSYGSDQPPKFIHLQYTPRGESKGRVCLVGKGVTFDSGGLSI
                     KPSDAMMGMKMDMGGAAAVLSALEGAARLGAPWEVHAIVAATENMTGGSAYKLGDVLT
                     ASNGKTIEIDNTDAEGRLTLADALVYAGKLQPTYIFDFATLTGACIVALGPHIAGVMS
                     KDDDLCAQWLAAGERAGEDAWRLPLPEVLKEQLKSPIADMRNTGERYGGAITAGLFLS
                     EFTEGQRWVHVDIAGPAMVRKPFGVNEEGGSGYAVATILEFLR"
     gene            complement(1598..>2089)
                     /locus_tag="VGB85_25015"
     CDS             complement(1598..>2089)
                     /locus_tag="VGB85_25015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137378.1"
                     /translation="SRSFTSSLSRLVAGLMLCGTLACAMTPERLPDLDRKFYYNLPNN
                     NDRAAFLKVQESQRQNFLEQKGLWARWSELSNEEREAVKRGEVKVGHKEFVAFMVWGP
                     PADSQGSKSETRAVRFHTFIRCTSGPRIGQYVMNNLDCDGTSSEIEIAVENNLITEIK
                     FLN"
BASE COUNT          330 a          781 c          683 g          295 t
ORIGIN      
        1 ctccacgctt gcgatggccc cgcgggtgag cgacaagacc aatgccccat gctgaccatc
       61 gactggacct cccaggaccc cacggcggcc gccctgcaga ccctcgcggt ggccatcccc
      121 ggtcgcgaca acgcgcccgc cgtcgaacaa ctgttcggcg ccgccaccgc ccaggccgcg
      181 accgccgcga gattccgcgg ccgcccgggc gagcaattcc gcttcacccg cgagcaggcc
      241 ggcgccctcc agacggtcgt gctgctcggc gtcggcgccg accccctggc gcccgggcgc
      301 tggcgaaccc tcgcccacgc cgcggtgcgc gccgccagcg aggtcggcag cgaacacctc
      361 gtgctcgacc tgcgccaggt cgccccgctc gccgccgtca tcaccgctcc ccagctcggc
      421 aacctgctgt gccagggcct cgagctcggc acctaccgct acgaccgcta catcgccgag
      481 gcgcgccgca agccgagcac cgtcaagcgc gtgagcgtcg tcggggtcga ccccgccggc
      541 aacgagggcg tcgaccgcgg ccagacgatc gccagcgccg tcgcccgcgc ccgtgacctc
      601 gtcaacggcc ccgccaacga ggtcacgccg acctacctcg cccgcttcgc cgccgacgtc
      661 gtcacccgcc tcgggggcgc cgccgacgtc aagctgaccg tgctcgagcg cgaggactgc
      721 gagaagctcg gcatgggctc gtacctcgcc gtgtcctacg gcagcgacca gcccccgaag
      781 ttcatccacc tgcagtacac cccgcgcggc gagagcaagg gccgcgtgtg cctggtcggc
      841 aagggcgtca ccttcgactc cggcggcctg tcgatcaagc ccagcgacgc gatgatgggc
      901 atgaagatgg acatgggcgg cgccgcggcg gtgctcagcg cgctcgaggg cgcggcccgc
      961 ctcggggctc cgtgggaggt ccacgcgatc gtcgcggcga ccgagaacat gaccggcggc
     1021 tcggcctaca agctcggcga cgtgctcacg gcgtccaacg gcaagacgat cgagatcgac
     1081 aacaccgacg ccgagggccg cctgaccctc gccgacgcgc tcgtctacgc cggcaaactt
     1141 cagccgacct acatcttcga cttcgcgacg ctgaccggcg cctgcatcgt cgccctcggg
     1201 ccgcacatcg ccggcgtcat gtccaaggac gatgacctgt gcgcgcagtg gctggccgcg
     1261 ggcgagcgcg ccggcgagga cgcctggcgc ctgcccttac cagaggtgct caaggagcag
     1321 ctcaagagcc cgatcgccga catgcgcaac accggcgagc gctacggcgg ggcgatcacc
     1381 gccggcctgt tcctgtcgga gttcaccgag ggccagcgct gggtgcacgt cgacatcgcc
     1441 ggcccggcga tggtccgcaa gcccttcggc gtcaacgaag agggcggctc cggctacgcc
     1501 gtggcgacga tcctcgagtt cctgcgctga cgcgcgcgaa gggacatggc ctgcaggtca
     1561 tgtcccttcg aacagcttcg aagctgccgc gagcgcgcta gttgaggaac ttgatctcgg
     1621 tgatcaggtt gttctcgacc gcgatctcga tctcgctcga ggtgccgtcg cagtcgaggt
     1681 tgttcatcac gtactgcccg atccgcgggc cgctggtgca gcgaatgaag gtgtggaagc
     1741 ggaccgcgcg ggtctccgac ttgctgccct ggctgtcggc cggcggaccc cacaccatga
     1801 acgcgacgaa ctccttgtgg ccgaccttga cctcgccgcg cttgaccgcc tcgcgctcct
     1861 cgttcgacag ctccgaccag cgcgcccaca ggcccttttg ctcgaggaag ttctgacgct
     1921 gcgactcctg caccttcaag aacgccgccc ggtcgttgtt gttgggcagg ttgtaataaa
     1981 acttacggtc cagatcgggc aatctctccg gcgtcatggc gcaggccagg gtgccgcaga
     2041 gcatgagacc ggcgaccagg cgcgacagcg aggaggtgaa ggagcgaga
//
LOCUS       DASWNJ010001505         1534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_409106, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001505 DASWNJ010000000
VERSION     DASWNJ010001505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409106"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="VGB85_25020"
     CDS             complement(<1..1104)
                     /locus_tag="VGB85_25020"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137379.1"
                     /translation="MNILILSPGYPAEMPQFTRGLAEVGAKVFGVGDQGPGALPELVQ
                     RHLSGYLPVRSLWDEAAVVDAVRHWPAAASLDGVECLWEPGMLLAARLREALHLPGLS
                     VAQTVPFRDKEAMKQVLDRAGLRTPRHIRCASVHACWEAAEKIGFPIIIKPIDGAGSA
                     DTYRVESSDELRNILPRLRHVPEVSVEEFVDGDEFTFDTVTIQGQVAYHNIAWYRPRP
                     LVARSHEWISPQVIALRHVDQPALAGGVELGLQVIKALGFQSGFTHMEWYRKADGEVV
                     FGEIGARPPGAHQVDQMNVACDFDVYREWGRAVAFGRFEAQIDRKYNVSNIYKRAHGE
                     GHIARISGLDRIERLYGEHIAWTKLLPVGAPRRN"
     gene            1337..>1534
                     /locus_tag="VGB85_25025"
     CDS             1337..>1534
                     /locus_tag="VGB85_25025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137380.1"
                     /translation="MFGGSPANPGVAPSYFHGQPIALDSIRELVAAALAFRKEEPWQT
                     FDAEQFIDVRVPALGIDAGSVS"
BASE COUNT          243 a          527 c          538 g          226 t
ORIGIN      
        1 gttgcgacgc ggggcgccga ccggcaagag cttggtccac gcgatgtgct cgccgtacag
       61 ccgctcgatg cgatcgagtc cgctgatcct ggcgatgtgg ccctcgccgt gggcccgctt
      121 gtagatgttg ctgacgttgt acttgcgatc gatctgcgcc tcgaagcggc cgaacgcgac
      181 ggcgcggccc cactcgcggt acacgtcgaa gtcgcaggcg acgttcatct ggtcgacctg
      241 gtgcgcgccg ggcgggcgag caccgatctc gccgaacacg acctcgccgt cggccttgcg
      301 gtaccactcc atgtgggtaa agccggactg aaagccgagc gccttgatca cctgcagtcc
      361 gagctcgacg ccgccggcga gcgccggctg atcgacgtgg cgcagcgcga tcacctgcgg
      421 cgagatccat tcatgactgc gcgcgacgag cgggcgcggg cgataccagg cgatgttgtg
      481 atacgcgacc tggccctgga tcgtgacggt gtcgaaggtg aactcgtcgc cgtcgacgaa
      541 ctcctcgacg ctgacctcgg gcacgtggcg caggcgcggg aggatgttgc gcagctcgtc
      601 gctcgactcg acccggtagg tgtccgccga gccggcgccg tcgatcggct tgatgatgat
      661 cgggaagccg atcttctcgg cggcctccca gcaggcgtgc acgctggcgc agcggatgtg
      721 ccgcggggtc cgcaggccgg cgcggtcgag cacctgcttc atcgcctcct tgtcgcgaaa
      781 cggcaccgtc tgcgcgacgc tcaggcccgg caagtgcagc gcctcgcgca gccgcgccgc
      841 cagcaacatg cccggctccc acagacactc gacgccgtcg aggctcgccg ccgccggcca
      901 gtgacggacg gcgtcgacca ccgcggcctc gtcccacagc gagcgcaccg gcaaataacc
      961 actgaggtgg cgctgcacca gctccggcag ggcgcccggc ccctgatcac cgacgccgaa
     1021 caccttggcg ccgacctcgg ccaggccgcg cgtgaactgc ggcatctcgg cgggataacc
     1081 gggggacagg atcaggatgt tcatgcgagc ccgcccttgc gtatcagcaa tcgccgggcg
     1141 agcgcaattc gtggcgtgag gggatgccag ggggacgtac cgggcttgcg agcgaaaacg
     1201 ctcgtgctat gttgcgcgta tgagcgggag aaaacgcagc aagtcacgcg cacagcgaac
     1261 ccggacgagc ccgcgacccg gccggccccg acccgaacag gcggccgggt cggcagatgc
     1321 ggtggtcgag gccgggatgt tcggcggatc gccggcgaac cccggggtag ctccgtcgta
     1381 ctttcacggc cagccgatcg cgctggactc gatccgcgag ctcgtcgcgg cggcgctcgc
     1441 gtttcgcaag gaagagccgt ggcagacctt cgacgccgag cagttcatcg atgtccgggt
     1501 cccggcgctc ggcatcgacg ccggcagcgt gtcg
//
LOCUS       DASWNJ010001506         1808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_378376, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001506 DASWNJ010000000
VERSION     DASWNJ010001506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378376"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VGB85_25030"
     CDS             complement(<1..211)
                     /locus_tag="VGB85_25030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137381.1"
                     /translation="MDWRVRVAAITDLGPARSYNEDAVAVFTAAAAPSLDLAVTRLLP
                     CDRDVGLLVIDGMGGQHTGDVPCGLA"
     gene            complement(227..1231)
                     /locus_tag="VGB85_25035"
     CDS             complement(227..1231)
                     /locus_tag="VGB85_25035"
                     /inference="COORDINATES: protein motif:HMM:NF022723.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3080 family protein"
                     /protein_id="HEY0137382.1"
                     /translation="MLLRRSVLLACLAVVGCEVRTPRERALAGYVAAIDPDATVPEFT
                     SRLRRLPPYPQRSVELARHTTDIGGFLHVQGCALGERIGERNSGLGRVMVGSQRWVYE
                     ARFLHDAGVCLPVMEASERAELQVVVDEKRAGLPAVVWNAIWGGPEIQHLLALSGRDL
                     DPGATGAAGPAADALATLRRLAEDPLHVTSHELEGALGSLRGNTVGGDVLRALEVLAH
                     FLGAAADRLEAAAGAPDCPRLRAVFERDYVGELQPYIARTHRAGATLLPVLRDLYDET
                     AARLPAELPALAAYREQLAPAGVWARYEAALARHTAAWRVIGERCDFLPGAPSSPAGP
                     "
     gene            complement(1350..>1808)
                     /locus_tag="VGB85_25040"
     CDS             complement(1350..>1808)
                     /locus_tag="VGB85_25040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137383.1"
                     /translation="LVERRDGFAVIGTAAGASFHLSQGAVAHGDIGVFLETRLPRAPW
                     LVCELFASSSPLAQTARRPGQRVTLARPPEGRADHYLQLWSNHRAQAPALMDEAVRAA
                     ATQLPRPYLLYQNGIVVLIWPCAALPGDTQLDAAIACIEALGRACQKLSS"
BASE COUNT          247 a          639 c          710 g          212 t
ORIGIN      
        1 cggcgaggcc gcacgggaca tcgccggtgt gctggccgcc catgccgtcg atgaccagca
       61 ggccgacgtc ccgatcacag gggagcagcc gcgtcaccgc gaggtcgagg ctcggcgcgg
      121 ccgcggcggt gaacaccgcg accgcgtcct cgttgtagga gcgcgccggc ccgagatcgg
      181 tgatcgccgc gacccgcacc cgccagtcca tgcgtcccac gctacttcag ggcccggccg
      241 gggacgaggg ggcgcccggc aggaagtcac agcgctcgcc gatcacccgc caggcggcgg
      301 tgtgacgcgc gagcgcggcc tcgtatcggg cccacacgcc cgcgggcgcg agctgctcgc
      361 ggtacgcggc gagcgcgggc agctcggcgg gcagccgcgc ggctgtctca tcgtacaggt
      421 cgcgcaggac aggtagcagg gtcgcgccgg cgcggtgggt gcgggcgatg tagggctgga
      481 gctcgccgac gtagtcgcgc tcgaacaccg cgcgcaggcg cgggcagtcg ggcgcgccgg
      541 cggccgcctc gaggcggtcc gcggcggcgc cgaggaagtg ggccaggacc tcgagcgcgc
      601 gcaggacgtc cccgccgaca gtgttgccgc gcaggctgcc gagcgcgccc tcgagctcgt
      661 ggctggtgac gtgcagcggg tcttcggcga ggcggcgcag ggtcgccagc gcgtccgcgg
      721 cgggcccggc cgcgccggtc gccccgggat cgaggtcgcg accggagagc gcgagcaggt
      781 gctggatctc cggcccgccc cagatcgcgt tccacacgac cgcggggagc ccggcgcgtt
      841 tctcgtcgac gactacctgc agctcggccc gctcggacgc ctccatcacc ggcaggcaga
      901 ccccggcgtc gtgcaggaac ctggcctcgt agacccagcg ctgcgagccg accatcacgc
      961 gccccaggcc gctgttgcgc tcgccgatcc gctcgccgag cgcgcagccc tggacgtgga
     1021 ggaagccgcc gatatcggtg gtatgccgcg ccagctcgac gctgcgctgc ggatacggcg
     1081 ggagccgccg caacctcgac gtgaattcgg ggaccgtcgc gtcggggtcg atcgccgcga
     1141 cgtagccggc gagggcgcgc tcgcggggcg tacggacctc gcagccgacc acggcgaggc
     1201 acgcgagcag cacggatcgc cggagcagca cgccggcagt ctgtggccgg tcgccgggat
     1261 cgacaaggcg cggcgcgaga tcgtggtgtg tgcgatcggg atgatccgct cgatcagctc
     1321 gatcagctcg atcagctcga tccgctcgat cagctcgaca gcttctgaca ggcgcggccg
     1381 agggcctcga tgcaggcgat cgcggcgtcg agctgggtgt cgccgggcag cgcggcgcac
     1441 ggccagatca gcacgacgat gccgttctga tacagcaggt aggggcgcgg cagctgggtg
     1501 gcggcggcgc gcacggcctc gtccatcagc gcgggtgcct gggcgcggtg gttcgaccac
     1561 agctggagat agtggtcggc gcggccctcc ggcgggcgcg ccagggtgac gcgctgaccc
     1621 ggacggcggg cggtctgcgc cagcggcgag ctgctggcga acagctcgca caccagccac
     1681 ggcgcgcgag gcagccgcgt ctcgaggaac acgccgatgt cgccgtgggc gacggctccc
     1741 tgactgaggt ggaagctggc gccggcggcg gtgccgatca ccgcgaagcc gtcgcggcgc
     1801 tcgacgag
//
LOCUS       DASWNJ010001507         2228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_592328, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001507 DASWNJ010000000
VERSION     DASWNJ010001507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592328"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VGB85_25045"
     CDS             complement(<1..194)
                     /locus_tag="VGB85_25045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137384.1"
                     /translation="MFVWTVSSVSLCAARGRALALTLAALGCGACAGTSPVPEDSDSG
                     AVATTGGSAADDGDPTEAASS"
     gene            537..1028
                     /locus_tag="VGB85_25050"
     CDS             537..1028
                     /locus_tag="VGB85_25050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137385.1"
                     /translation="MALTAAAVYLIVTGAVFGLAIGLLAVGRAMVGKWVVLPALAVPA
                     GVALALWLGADMLAARAQAVLDAVVLAAAERDLILHDLGEVGHAGPVALVVVLPLLFL
                     DLGAILVDPGVLGALGLLVLALAAVVLAGGVPAALISMGVVLAGYVRGLMGRWRMWVK
                     IQV"
     gene            complement(1039..1539)
                     /locus_tag="VGB85_25055"
     CDS             complement(1039..1539)
                     /locus_tag="VGB85_25055"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HEY0137386.1"
                     /translation="MTISDPNRDESQEPDKQVGQLLAEAHMILPGLLMLLGLQIIAVF
                     NDVFQDGLEPHDHALHLVALFLLATGAALVVTPAAYHRQVAPYGIPADFAKLASRFAT
                     AALVPLMFALSIDAYLVGKLALGDLPLDKSLTAIAIAVALLMMFSALWFVFPWVMTRR
                     RQRAAR"
     gene            complement(1814..>2228)
                     /locus_tag="VGB85_25060"
     CDS             complement(1814..>2228)
                     /locus_tag="VGB85_25060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137387.1"
                     /translation="TSGSSTTTGDTTTTSDTTITTDTTMGVSASSSGSESTDPMPATD
                     GSATGFTTGSFPTTGGDGGDESVTGTPGTGPSPTEAGGDEGPDTTDGASSTSGDPGTA
                     TDTGCGCQTDTRNSNAWLQLIPWLGLIGLRRRRRT"
BASE COUNT          273 a          694 c          895 g          366 t
ORIGIN      
        1 gagctggcgg cctcggtcgg gtcaccgtcg tccgccgcgg agccgccggt ggtcgcaacc
       61 gcacccgagt cactgtcctc ggggacaggt gaggtacctg cgcaggcgcc acagccgagc
      121 gccgcgaggg tcagcgcgag cgcccgacca cgcgccgcac acagcgatac cgatgacacc
      181 gtccagacga acacgcgggc agcttttcac aggcgggccg cgctgacaag ccggggatcg
      241 ccgccgacac aggccggggc cgtctgccac cgggctgcca ccgggctgcc acctggcagg
      301 tgggtgatcg ggtgatccag gccgcgccgg gggcgggagg acgcgtgcta gcgtgatgca
      361 cgatggggtc ggtacactgg cgaggcttcg cacgcgtgga gctacggcgg ctcggcgccg
      421 cgttcgcgcg cggctgtacc ttcggcacgc tggcctatgc gctgatcctc tgggtgctcg
      481 tcgccggtaa tctgctgttc gacgggtcgt tcgcgcgaac gcccctcggg acgctggtgg
      541 cgctgacggc ggcggccgtg tacctgatcg tcaccggcgc ggtgttcggt ctggcgatcg
      601 gcctgctggc agtcggacgc gcgatggtcg gcaagtgggt ggttttgccc gcgctcgccg
      661 tgccggccgg tgtcgcgctg gcgctgtggc tcggcgccga catgctcgcc gcgcgggcgc
      721 aggcggtgct cgatgcggtg gtgctcgctg ccgccgagcg tgacttgatc cttcacgatc
      781 tcggcgaggt cggacacgcg gggccggtcg cgctcgtcgt cgtgctgccg ctgttgttcc
      841 tcgatctcgg ggcgatcctc gtcgatccgg gggtgctcgg ggcgctcggc ttattggtgc
      901 tcgcgctcgc ggcggtggtg ctcgcgggag gcgtgccggc ggcgctgatc tcgatggggg
      961 tggtgctcgc gggctatgtt cgcgggttga tgggacgctg gcggatgtgg gtgaagatcc
     1021 aggtgtagtg agggcgcgct acctggcggc tcgctgtcgg cggcgcgtca tgacccacgg
     1081 gaacacgaac cacagggcgc tgaacatcat cagcaacgcg acggcgatcg cgatcgcggt
     1141 caatgacttg tcgagcggga ggtcgccgag cgcgagcttg ccgacgaggt aggcgtcgat
     1201 cgacagggcg aacatcagcg gtacgagggc cgcggtggcg aagcgtgagg cgagcttggc
     1261 gaagtcggcc gggatgccgt agggggcgac ctggcggtga taggccgcgg gcgtgacgac
     1321 gagcgcggcg ccggtggcga gcaggaacag ggcgaccagg tgcagcgcgt ggtcgtgggg
     1381 ctcgaggccg tcctggaaca cgtcgttgaa gacggcgatg atctgcaggc cgagcaacat
     1441 caacaggccc ggcaagatca tgtgcgcctc ggcgagcagc tggcccacct gcttgtccgg
     1501 ctcttgcgac tcgtcccggt tcggatcgct tatcgtcatg ctcaggtcct cctttagcga
     1561 cgcgactctg tatggagagt cctcgcgtac gggcaagcag cgcggccggc gacgtcgcgc
     1621 gcgcgcggcc gaacgccgtc gctgtcggcg gcatgaagcg gcctgcggcg cgggcgaacg
     1681 tgcggtctag cctgcgaggc gcgggcgcgt gacgagcccg ggactgcgtt ctgccggagc
     1741 atgcgcggcg aacgcccgaa gcggtgttcg ggacaccgct tcagggagaa cccggtggtc
     1801 cggttgcgag ggatcaggtg cgacggcggc ggcgcaggcc gatcaggccg agccagggga
     1861 tcagctgcag ccaggcgttg ctgttgcggg tgtcggtctg gcagccgcag ccggtgtcgg
     1921 tggcggtgcc ggggtcgccg ctggtgctgc tcgctccgtc ggtggtgtcc gggccctcgt
     1981 cgccgccggc ctcggtcggc gagggtccgg taccgggggt gccggtgacc gactcgtcgc
     2041 cgccgtcgcc accggtggtc gggaagctgc cggtggtgaa gccggtcgcc gagccgtcgg
     2101 tggccggcat cgggtcggtg gactccgagc cgctgctgct cgcgctgacg cccatggtgg
     2161 tgtcggtggt gatggtggtg tcgctcgtcg tcgtggtgtc accagtcgtg gtcgagctgc
     2221 ccgaggtc
//
LOCUS       DASWNJ010001508         1580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_592371, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001508 DASWNJ010000000
VERSION     DASWNJ010001508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592371"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1335)
                     /locus_tag="VGB85_25065"
     CDS             complement(<1..1335)
                     /locus_tag="VGB85_25065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137388.1"
                     /translation="MTRATHRRLGAAKLGVAAILLLACNSGEPEPPQLQRLRLPSGAL
                     LTPDNNWLLVTNSNLDQREDASTLVAIDLRQLDKALVDTPLAPDAVPTLSRPCRRTVD
                     DAIAPLECDAAQLIARKHAIRLPSGAGNIALDRPAGESGPLRLLIPSGIARRITWVDV
                     VPDGLGGLDLDCGQLGEGDCDPVHVLEYLDNRPAEDRLPADPARIFIDRGGFRYAYLP
                     HLLGSAITLIALDAEFGPKITDIETEFYRSDPINNNTFSGGFAVAQRACDPADPPAVT
                     RDCTRPMLYTTHRFWPGVRTFTVRTGQDTIIPGGDQALVGVNPTQSKDRPFMADLEFE
                     DPATGERLLVVHTTPPSLSLVDTRLDDDKDPINRQIATVSLCSNPSNPNILAIDRPPV
                     GERLALVSCYSDDQIAVVGLGAFQVISTLDVDDGPNELVIDDARRRLYVVNTP"
     gene            complement(1376..>1580)
                     /locus_tag="VGB85_25070"
     CDS             complement(1376..>1580)
                     /locus_tag="VGB85_25070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137389.1"
                     /translation="LALTLATPVLLTAATLDLGLRLACRGVAVPVGEALRPWLCAAAG
                     LVALAAAWAAYPDAGLRAWPGVP"
BASE COUNT          218 a          541 c          605 g          216 t
ORIGIN      
        1 cggcgtgttg acgacgtaga gccgccggcg cgcgtcgtcg atgaccagct cgttgggccc
       61 gtcgtcgacg tcgagcgtgc tgatgacctg gaacgccccg agcccgacca ccgcgatctg
      121 gtcgtcggag taacagctga ccagcgcgag ccgctcgccg accggcgggc ggtcgatcgc
      181 caggatgttg gggttgctgg ggttgctgca cagcgacacg gtcgcgatct ggcggttgat
      241 cgggtccttg tcgtcgtcga gccgcgtgtc gaccagcgag agcgagggcg gcgtcgtgtg
      301 cacgaccagc aagcgctcgc cggtcgccgg gtcctcgaac tcgaggtcgg ccatgaacgg
      361 ccggtccttg gactgcgtcg ggttgacccc gacgagcgcc tggtcaccgc cggggatgat
      421 cgtgtcctgg cccgtgcgca cggtgaaggt gcgcacgccg ggccagaagc ggtgcgtcgt
      481 gtacagcatc gggcgcgtac agtcgcgggt gaccgccggc ggatcggcgg ggtcgcaggc
      541 ccgctgcgcg accgcgaagc cgcccgagaa ggtgttgttg ttgatcgggt cggaccgata
      601 aaactcggtc tcgatatcgg tgatcttcgg cccgaactcg gcgtcgaggg cgatcagcgt
      661 gatcgcgctg ccgagcaggt gcggcaagta ggcgtaacga aacccgccgc ggtcgatgaa
      721 gatccgcgcc gggtcggccg gcagccggtc ctccgcgggc cggttgtcga ggtactcgag
      781 tacgtgcacc ggatcgcagt caccctcgcc gagctggcca cagtcgaggt cgagcccgcc
      841 gaggccgtcg ggcacgacgt cgacccaggt gatccggcgg gcgatgccgg aggggatcag
      901 caggcgcagc ggccccgact cgcccgccgg ccggtcgagg gcgatgttgc cggcgccgct
      961 cggcaggcgg atcgcgtgct tgcgggcgat cagctgcgcg gcgtcacact cgagcggggc
     1021 gatggcgtcg tcgacggtgc gccggcacgg ccgactcagc gtcggcacgg cgtcgggcgc
     1081 gagcggggtg tccacgagcg ccttgtcgag ctgccggaga tcgatcgcca ccagcgtgct
     1141 cgcgtcctcg cgctggtcga ggttgctgtt ggtcaccagc aaccagttgt tgtcgggcgt
     1201 cagcagggcg ccgctcggca ggcgcagtcg ctgcagctgc ggcggctccg gctcgcccga
     1261 gttgcaggcc agcagtagga tcgccgcgac cccgagcttc gcggcgccga gccggcggtg
     1321 ggtcgcgcgt gtcatgcccg ctgcgagcat cgcacccatg acgccccccg ccaagtcagg
     1381 gcacaccggg ccacgcccgc aagcccgcgt ccgggtaggc cgcccaggcc gccgcgagcg
     1441 cgaccaggcc cgccgcggcg cacagccacg gccgcagcgc ctcgccgacc ggcaccgcga
     1501 cgccgcggca ggccaggcgc agcccgaggt cgagggtcgc cgcggtcaac agcacggggg
     1561 tggccagcgt cagcgcgagc
//
LOCUS       DASWNJ010001509         9887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_310411, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001509 DASWNJ010000000
VERSION     DASWNJ010001509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 9887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9887
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310411"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1641
                     /locus_tag="VGB85_25075"
     CDS             <1..1641
                     /locus_tag="VGB85_25075"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0137390.1"
                     /translation="WTRVATYDYTTSPNEKRNVEAVVRFANDRWTVAIADLSDATGDR
                     RSSQVSLVLGQLLPRGFERESFAGKTAHKLDQARLGELGNFVVTAQELLRVPGVSIGI
                     VQDGEVVFSGGFGVREHGKPDRVDGDTRYIIASNTKAMTTLMLGKLVDEGKLTWDTAA
                     SSLLPAFKLGDAETTRKVQVKHLICACTGMPRQDLEWLLEHEALTPEGALVALGGMQP
                     TSKFGALFQYSNPLAAAAGFIGGHVAYPELELGAAYDKAMQTRVFAPLGMTATSFHTK
                     AAQTGNYARAHAPDLQDRPTLAITAINDAIIPLRPAGGAWSTVNDVLKYVQMELAVGK
                     LPGGEQYIARDTLLARRAPQVAVGNDETYGMGLTVHTKYGVTVVNHGGDLIGYHSDMM
                     WLPDHGVGAVVLTNGDPGYILRDKFRRRLLELLFDGKPEAAADLAAAGKSYFAGRVAE
                     REEWTVPADPGVVAGLATKYTSKELGDVTVIRSGAATVLDFGEWRSEVASKQNPDGSI
                     SLVTAVPGLEGLALVVGTGEKQTLTVRDAQHEYLFTAQ"
     gene            1699..2712
                     /locus_tag="VGB85_25080"
     CDS             1699..2712
                     /locus_tag="VGB85_25080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137391.1"
                     /translation="MRRTPACSLAFALLLACKGGEPTTTESATDAGSTTSDEPTTGAT
                     STTGATSSTSDDPTSTQGTQGLTDSPTSEPDTGTTASEPGCEDADGDGFGVGCAGGED
                     CDDASDRCALDCIDVDMNGIPECAEAPGTGVLLFTGSMGGGPETDLYVDSLATLYMQA
                     GHETTIANGFPGDFAQTTGTMLILNPLEPLGQKVLLGARYLLLRGGRVVLIMEHCKNG
                     CYGNAAEDNALLEALGSSIRLSGEGGAPLAETPLTLAAAPPTAGLSTIVTYYSGSLTV
                     GDGVALGTMDGGAGDVVLGYETLYNGDIVVAADSSIFGFMLDKGDNTAFVVGLADTVL
                     GGP"
     gene            2882..4570
                     /locus_tag="VGB85_25085"
     CDS             2882..4570
                     /locus_tag="VGB85_25085"
                     /inference="COORDINATES: protein motif:HMM:NF015612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 71 protein"
                     /protein_id="HEY0137392.1"
                     /translation="MRTSPARGSLLLSTILITACGGGDPGATTSGGSSSSSSSTGSGT
                     TSVDPTTSGPPTSDPTTGSTTADPTGSTTSSSTADPTTDSDTSTDTSTGGGAEATPCW
                     PFDAPPLSSLRAAPKKVFGHYFSPYPISLDNKDPEVDYYQKNYLQPTGENSKFAYCGG
                     FIKQRPIPQPPRPADVDYELANFEEEVRRAAAIGLDGFTYDILNTSGTHWDRLLKLLD
                     AAANVDPEFRIVLMPDMTATYSGTDMEAQVAFVDSMLTVASHPAVYHVDGALMLAPFA
                     ADKRSPAWWASAMQALADAGVDSVLWPVFVSPWPAASMSFQAEVPLYGTSSWGPATIP
                     GATNYATNAVTAHDLGLRWMSPVRPQDSRPKDLVYSEAGNTQTMRLLWDGAIEGDAEW
                     VQLITWNDYSEATELSPSSGTQWALFDLTAYYVTWFKTGAQPPIVRDALYYAHRLHAT
                     TAMPDLSLQSEVYKQVNGGTPLDEIELLAFLTAPATLEIEVDGAVQAMDADAGIQSFR
                     VPLVEGTPKFRVVRAGEVVTAVDSAFPISNTIVYQDMLYRAGGSLPCDHSPLLQ"
     gene            4574..4942
                     /locus_tag="VGB85_25090"
     CDS             4574..4942
                     /locus_tag="VGB85_25090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137393.1"
                     /translation="MRRFNFAERIMLQHAHPAKLSLDCLGIVFGCHFLWTHRLSWALL
                     SLFGMSILGNLVVWRVDLDRLAGTALGRWMLGQARPVNLIVRSLGATVLAGGLWWHSA
                     LYIAAGIAVIIAARRVGAGA"
     gene            4978..5352
                     /locus_tag="VGB85_25095"
     CDS             4978..5352
                     /locus_tag="VGB85_25095"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY0137394.1"
                     /translation="MSDAVESIADQETLEALMSPEGPAAIIDFWAGWCGPCKMMAPQY
                     QAVAVELAGRPIKFYKLDTEHHPELARAFNIRSLPTLLLIHRGEIKDALIGAQSAATI
                     RKKAEWLLHRVEGKGLWARLFG"
     gene            complement(5374..6129)
                     /locus_tag="VGB85_25100"
     CDS             complement(5374..6129)
                     /locus_tag="VGB85_25100"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY0137395.1"
                     /translation="MRSPLPLLTLTLAACSLAPAPTPMTKTFAQTRPGAAPARCLVVL
                     LPGAGDRASTFREEGFVAEIQRSGASADIVAADATIGYYYRGVAVERIATDVVGPLRG
                     RYEQVWLMGVSMGGFGSLHYAQQHAGEITAVAVFAPYLGSRRLGEEIRDAGGLARWTP
                     DLPAPVTKKNYQRQLWSWLHRVTSGAQPGPTIYLGYGDDDRLAPQDALLAAALPPAQV
                     FHAKGGHDWPVWRELLRQFLQRSQFTTRCAPPG"
     gene            6281..6676
                     /locus_tag="VGB85_25105"
     CDS             6281..6676
                     /locus_tag="VGB85_25105"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEY0137396.1"
                     /translation="MAIELTLLGYTLVLALVYIMAAAQARTKQYGTTWNTGARDEAMP
                     PLNPLPARLVRAQANLFETLPLFAAAVLAVVVAGRTGVLSEVGAWMYLLGRVVYLPLY
                     ALGVPVVRTVVWMISSLGLVLVLWQLLCG"
     gene            6766..7809
                     /locus_tag="VGB85_25110"
     CDS             6766..7809
                     /locus_tag="VGB85_25110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137397.1"
                     /translation="MALLTPPTSDDRQIFLDPGARFVPTTLLDDAYVRYEETQNPSAS
                     NHVRLDDGLELGIVSSAVPEIARGISSMLSRSLDDARNDHGGHLPIAVVQRVQSEIIS
                     PYGVANLWGTTGHRFVLSRRIDGRTSELLATILVGRRKGTIFFLTGRYNNLRHSQIAD
                     EVDFDQPAGTDPGQRWFDQFVFPDLERFKPTAYHQIANFVVAKEHRGEGLAERLLAAI
                     VGKYAKDDIDARGAPIEHSQHLLCGRGLWQIGDPPWLARMEKLGFYLRWGAESFFIEH
                     DWAPLPPTFDRGRPLSNREYNRSFGLPHRYQEGRSPPASSEHLLDRVDTVVSLSQDPR
                     AKLQYFQTMFDFT"
     gene            7806..8666
                     /locus_tag="VGB85_25115"
     CDS             7806..8666
                     /locus_tag="VGB85_25115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137398.1"
                     /translation="MKTHLRQARDVTTQQVASFTGEGAYHGSTRARPAPVQAAPDVVV
                     EEMRPCSLDKYDASEPREIDTFDAHFPARYEVLDALPVPTQEQIVADGVTFCWCPIDE
                     AIREAGPLTRSVLEAMQRRVTGSKRYVYIDSKIQYFEHGDVPVDSQHWHVDGSIAVRD
                     ERARRLGHALLHDMRARLEGPAAPPTYLSYQSSEHCATLFVTEPVALVLPELIPSFDE
                     LDRQVRALAPACVAQPAATIARFDGLSLHRAVAASAAGWRLWIRCAETDRRVELTASI
                     IDCYGTVYRT"
     gene            8827..9792
                     /locus_tag="VGB85_25120"
     CDS             8827..9792
                     /locus_tag="VGB85_25120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137399.1"
                     /translation="MSFDSKLYTRDRPILDETQTSWLARDGAYTWEYEKWLFGGMIVD
                     VEPYEFVAGDGDPLGEEFPEADEVPDWTREIRWVVCVSGSRPDDHFFNWFDTLATDAR
                     GVIWTPYDKHSLEHVDGFEPRYVAWRMLLATRPIHCGHNDRARDLVRRDAKPEKEPAL
                     DVPVDLAEIDAAWRAARAIVYGAALATAADAGVRVSWAAAQHLHLAADPDADGPLWFS
                     MFDAPVRGRADAPAMLTLTAHPDPRIDALLRDPAGAAELRALASDTSDPLLRALAGLE
                     PAEPAPLPDSRELRNELVEFWERVVMLDPRLDPAELDDVRRPRMG"
BASE COUNT         1519 a         3579 c         3326 g         1463 t
ORIGIN      
        1 tggacgcgcg tggcgaccta cgattacacg acctcgccga acgagaagcg caacgtcgag
       61 gccgtggtcc gcttcgccaa cgaccgctgg accgtggcga tcgccgacct ctcggacgcg
      121 accggcgacc gacgcagctc gcaggtctcg ctcgtgctcg ggcagctgct gccccgcggc
      181 ttcgagcgcg agtcgttcgc cggcaagacc gcccacaagc tcgaccaggc gcgcctcggc
      241 gagctcggca atttcgtcgt caccgcgcag gagctgctgc gtgtccccgg cgtgtcgatc
      301 ggcatcgtgc aggacggcga ggtcgtgttc agcggcggct tcggggtgcg cgagcacggc
      361 aagccggaca gggtcgacgg cgacacccga tacatcatcg cctcgaacac caaggcgatg
      421 acgacgctga tgctcggcaa gctggtcgac gagggcaagc tgacctggga caccgccgcg
      481 tcgagcctgc tgcccgcgtt caagctcggc gacgccgaga cgacgcgcaa ggtccaggtc
      541 aaacacctga tctgcgcgtg caccgggatg ccgcggcagg acctcgagtg gctgctcgaa
      601 catgaggcgc tcacgcccga gggcgcgctc gtggccctcg gcggcatgca gccgaccagc
      661 aagttcggcg cgctgttcca gtactcgaac ccgctcgcgg cggccgccgg cttcatcggc
      721 ggacacgtcg cctaccccga gctcgagctc ggcgccgcct atgacaaggc catgcagacg
      781 cgggtgttcg cgccgctcgg catgacggcg accagcttcc acacgaaggc ggcgcagact
      841 ggcaactacg cccgcgcgca cgcgccggac ctgcaggacc gaccgaccct ggcgatcacc
      901 gcgatcaacg acgcgatcat cccgctgcga cccgcgggcg gcgcctggag caccgtcaac
      961 gacgtcctca agtacgtaca aatggagctc gccgtcggca agctgcccgg tggcgagcaa
     1021 tacatcgccc gggacacgtt gcttgctcgg cgggccccgc aggtcgcggt cggcaacgac
     1081 gagacttacg gcatggggct gaccgtgcac acgaaatatg gtgtcaccgt cgtcaaccac
     1141 ggcggcgacc tgatcggcta tcacagcgac atgatgtggc tgccggatca cggcgtcggc
     1201 gcggtggtcc tgaccaacgg cgatccgggc tatatcttgc gcgacaaatt ccggcgcagg
     1261 ctgctcgagc tgctgttcga cggcaaaccg gaggccgcgg ccgacctcgc ggccgccggc
     1321 aagagctact tcgccggcag ggtcgccgag cgcgaggagt ggaccgtgcc ggccgacccc
     1381 ggcgtggtcg ccgggctggc gacgaaatac accagcaagg agctcggcga cgtcaccgtg
     1441 atccgaagcg gcgccgcgac cgtgctcgac ttcggcgagt ggcgaagcga ggtcgcgtcg
     1501 aagcagaacc ccgacggatc gatctcgctg gtcaccgccg tgcccggcct cgagggcctc
     1561 gcgctcgtcg tcggcaccgg cgaaaagcag accctgaccg tgcgcgacgc ccagcacgag
     1621 tacctgttca cggcgcagtg acggcgcgct cctggacccg ccttgcccgg cgggtcgcgt
     1681 cctgataagc ccgcggccat gcgccgcacg cccgcctgtt cgctcgcctt cgccctgctg
     1741 ctcgcctgca agggcggtga gccgaccacc accgagtcgg cgacggacgc gggctcgacc
     1801 acgagcgacg agccgaccac cggcgccacg tcgaccaccg gcgccacgtc atcgaccagc
     1861 gatgacccga cgagcacgca gggcacgcag ggcctcaccg acagccccac cagcgagccc
     1921 gacaccggca ccaccgcgag cgagcccggc tgcgaggacg cggacggcga tggttttggc
     1981 gtcggctgtg caggcggcga ggactgcgac gacgcctccg accgctgcgc gctggactgc
     2041 atcgacgtcg acatgaacgg tatccccgag tgcgccgagg cgccgggcac cggcgtgctg
     2101 ctgttcacgg gctcgatggg cggcggcccc gagacggacc tgtatgtcga cagcctcgcg
     2161 acgctgtaca tgcaggcggg ccacgaaacg acgatcgcca acggcttccc cggcgacttc
     2221 gcccagacca ccggcacgat gttgatcctc aacccgctcg agccgctcgg ccagaaggtg
     2281 ctgctcggcg cccgctacct gcttctgcgc ggcggtcggg tcgtgctgat catggagcac
     2341 tgcaaaaacg gctgctacgg caacgccgcg gaggacaacg ccctgctcga ggctctcggc
     2401 agcagcattc gcctctcggg tgagggcggc gcgccgctgg ccgagacccc gctgaccctc
     2461 gccgccgcgc cgccgaccgc gggcctgtcg acgatcgtca cctactactc cggctcgctg
     2521 acggtcggcg acggcgtcgc gctcggcacg atggacgggg gcgccggcga cgtcgtgctc
     2581 ggctacgaga cgctctataa cggcgacatc gtcgtcgccg ccgacagctc gatcttcggc
     2641 ttcatgctcg acaagggcga caacacggcc ttcgtggtcg ggctcgcgga taccgtgctc
     2701 ggcggcccct gattccgcgg gctccaagcc ccacacgcgt ccgcagcgcc gctcgcgctg
     2761 cacgcggctc ggtgatccat tcggtcacca cccgtcgcca acccccacgg gcccgcgttc
     2821 gcgtcggtgc gcttgcgagc cttgacggca cgccgccgct tgggcctcct gaccgcagca
     2881 gatgcgtact tcaccggccc gcggatccct ccttctgtcc acgatcctga tcaccgcgtg
     2941 cggcggcggg gaccccggcg cgacgacctc cggagggtcc tcgagctcgt cgagctcgac
     3001 cggctccggc acgacctccg tcgatccgac gacctcgggc ccgcccacct cggacccgac
     3061 caccggatcg accaccgccg atccgaccgg atcgaccaca tccagttcca ccgccgatcc
     3121 gaccaccgac agcgatacct cgaccgacac gagcacgggc ggtggtgccg aggcgacccc
     3181 gtgctggccc ttcgacgcgc cgccgctgag cagcctgcgt gccgccccga aaaaggtctt
     3241 cggtcactac ttctcgccgt acccgatctc gctcgacaac aaggatcccg aggtcgacta
     3301 ttatcaaaag aactacctcc agccgaccgg cgagaactcg aaattcgcct attgcggagg
     3361 ctttatcaaa cagcgcccga tcccgcagcc gccgcgtccg gcggacgtcg attacgagct
     3421 cgccaacttc gaggaggagg tgcggcgcgc cgcggcgatc gggctcgacg gcttcaccta
     3481 cgacattctc aatacgagcg ggacccactg ggaccgcctg ctcaagctgc tcgacgccgc
     3541 ggccaacgtc gaccccgagt ttcgcatcgt gctgatgccg gacatgaccg ccacctactc
     3601 gggcaccgat atggaggcgc aggtcgcgtt cgtcgactcg atgctcaccg tcgcctcgca
     3661 cccggccgtg taccacgtcg acggcgcgct gatgctcgcg ccctttgccg ccgacaagcg
     3721 cagcccggcg tggtgggcct cggccatgca ggcgctcgcc gacgcgggcg tcgactccgt
     3781 gctgtggccg gtgttcgtct cgccgtggcc ggccgcgagc atgagctttc aggcggaggt
     3841 gccgctatat ggaacctcct cctggggccc cgcgacgatc ccgggcgcga ccaattatgc
     3901 cacgaacgcg gtcaccgccc acgacctcgg gctgcggtgg atgtcgccgg tgcgcccgca
     3961 ggactcgcga cccaaggacc tggtctacag cgaggccggc aacacccaga cgatgcgtct
     4021 gctgtgggac ggcgcgatcg agggtgacgc cgagtgggtg cagctgatca cctggaacga
     4081 ctactccgag gccaccgagc tgtccccgtc gagcgggacc cagtgggcgc tgttcgacct
     4141 gaccgcctat tatgtcacgt ggttcaagac cggcgcgcag ccgccgatcg tccgcgacgc
     4201 gctgtattac gcgcaccggc tgcacgcgac gaccgcgatg cccgatctct cgctgcaatc
     4261 ggaggtctac aagcaggtca acggcgggac gccgctcgac gagatcgagc tgctcgcgtt
     4321 cctgaccgcc cccgcgaccc tcgagatcga ggtcgacggc gccgtgcagg ccatggacgc
     4381 ggacgccggg atccagtcgt ttcgggtccc gctcgtcgag ggcaccccga agtttcgcgt
     4441 cgtgcgggcc ggggaagtcg tgaccgcggt cgacagcgcg ttcccgatct cgaacacgat
     4501 cgtctatcag gacatgctct accgcgccgg cggcagcctg ccctgcgacc acagcccgct
     4561 cctgcagtaa gcaatgcgaa gattcaactt tgccgagcgg atcatgttgc agcatgcgca
     4621 tcctgcgaag ttgtcgctgg attgcctggg gatcgtgttc gggtgccact ttttgtggac
     4681 ccaccgactg tcatgggcgc tgttgtcgct gttcgggatg tcgatcctcg ggaacctcgt
     4741 cgtgtggcgg gtcgacctcg atcggctcgc cggcaccgcg ctcgggcgct ggatgctggg
     4801 ccaggccagg ccagtcaatt tgatcgtgcg ctcgctcggg gccacggtgc tggccggcgg
     4861 cctgtggtgg cactcggcgc tgtatatcgc agcgggcatc gccgtgatca tcgcggcccg
     4921 gcgcgtcggc gccggtgctt gacccgcgcc ccgccagcct gcacccttgg cgcgatcatg
     4981 tccgacgccg tggagagcat cgccgaccag gagacactcg aggccctgat gagccccgag
     5041 gggccggcgg ccatcatcga cttttgggcc ggctggtgcg gcccctgcaa gatgatggcg
     5101 ccgcaatacc aggccgtcgc ggtcgagctc gcgggtcggc cgatcaagtt ctacaagctc
     5161 gacacggagc accaccccga gctcgcgcgc gccttcaaca tccgctcgct gccgaccctc
     5221 ctgctaatcc accgcggcga gatcaaagac gccctgatcg gcgcccagtc ggccgcgacg
     5281 atccgcaaga aggccgagtg gctgctgcac agggtcgagg gcaagggcct gtgggcgcgc
     5341 ctgttcgggt gagcccgacg gcgcgtcagc gcgtcagccc ggcggcgcgc agcgggtcgt
     5401 gaactgcgag cgctgcagga actgccgcag cagctcgcgc cacaccggcc agtcgtggcc
     5461 gcccttcgcg tgaaacacct gcgccggcgg cagcgcggcg gccagcagcg cgtcctgcgg
     5521 ggcgaggcgg tcgtcgtcgc cgtagccgag atagatcgtg gggcccggct gcgcgccgga
     5581 ggtgacccgg tgcagccagc tccacagctg gcgctgatag ttcttcttcg tgaccggcgc
     5641 gggaagatcg ggggtccagc gggccaggcc gccggcgtcg cggatctcct cgccgagccg
     5701 gcgcgagccg aggtacggcg cgaacactgc caccgccgtg atctcgccgg cgtgctgctg
     5761 ggcgtagtgc agcgagccga agccgcccat cgacacgccc atcagccaca cctgctcgta
     5821 gcgcccgcgt agcggcccga cgacatcggt ggcgatgcgc tcgacggcga cgccgcggta
     5881 gtagtacccg atcgtcgcgt cggccgcgac gatgtccgcc gaggcgccgc tgcgctggat
     5941 ctcggcgaca aagccctcct cgcggaacgt gctcgcgcgg tcgcccgcgc ccggcaggag
     6001 caccaccagg cagcgcgccg gcgcggcgcc tggccgcgtc tgcgcgaacg tcttcgtcat
     6061 cggcgtcggc gcgggggcca ggctgcaggc cgcgagcgtc agcgtgagga ggggcagcgg
     6121 cgagcgcatg tccctttgta gtcgcggtcc gccgctggcg cactggaatc gcgccacgcg
     6181 tccgctcact cgtcacggac cgcctggggc gtcacgcagg ccgccgtgtg gcgggccatg
     6241 gcccgtggtc cgtggtacat gggggtcagg ggatacgacc atggcgatcg aactgacact
     6301 gctcgggtat acgctggtgc tggcgctggt ctacatcatg gcggccgcgc aggcgcgtac
     6361 gaagcaatac ggcacgacct ggaacacggg cgcgcgcgac gaggcgatgc cgccgttgaa
     6421 cccgctgccg gcgcgcctcg tgcgggcgca ggccaacctg ttcgagaccc tgccgctgtt
     6481 cgccgcggcc gtgctcgccg tcgtcgtcgc cgggcgaacc ggcgtgctga gcgaggtcgg
     6541 cgcgtggatg tacctgctcg gccgcgtcgt ctacctcccg ctgtacgccc tcggcgtgcc
     6601 ggtcgtgcgc accgtcgttt ggatgatctc cagcctcggc ctcgtgctcg tgctgtggca
     6661 gctgctgtgc ggctgagcgc gcgcagttcc gggcgccggg gcgcgactgc gtccgcgcgc
     6721 gctcgccagc tttatcgcca cacgtcattc gtgtaatacg gacgtgtggc tctgctgaca
     6781 ccgccgacct ccgacgaccg gcagatcttc ctcgacccgg gggcgcgctt cgtcccgacg
     6841 acgctgctcg acgacgccta cgtccgctac gaggagacgc aaaatccgtc ggcgagcaat
     6901 cacgtccgcc tcgacgacgg cctcgagctc ggcatcgtct cgagcgccgt gccggagatc
     6961 gccaggggca tctcgtcgat gttgagccgc tcgctcgacg acgcgaggaa cgaccacggc
     7021 gggcatctcc cgatcgcggt ggtccagcgg gtccaaagcg agatcatctc gccctacggc
     7081 gtcgccaacc tgtggggtac gacgggccac cgcttcgtgc tctcgcgcag gatcgacggt
     7141 cgaacgagcg agctgctggc gacgatcctc gtcggccggc gaaagggcac gatcttcttc
     7201 ctcaccggtc gctataacaa tctgcgtcac tcgcagatcg ccgacgaggt cgacttcgat
     7261 cagcccgcgg gtaccgaccc ggggcagcgg tggttcgacc agttcgtatt ccccgacctc
     7321 gagcgcttca agccgaccgc gtatcaccag atcgccaatt tcgtggtcgc aaaggagcac
     7381 cgcggcgagg gcctcgccga gcgcctgctc gccgcgatcg tcggcaaata cgccaaggac
     7441 gacatcgacg cccgcggcgc gccgatcgag cactcgcagc acctgctgtg cgggcgcgga
     7501 ctgtggcaga tcggcgatcc accgtggctc gcgcggatgg agaagctcgg gttctacctg
     7561 cgctgggggg ccgagagctt cttcatcgag cacgactggg cgccgctgcc accgaccttc
     7621 gatcgcgggc ggccgctctc caaccgcgaa tataatcgat ccttcgggct gccacaccga
     7681 taccaggagg gccgctcccc gccggcctcg agcgagcacc tgctcgaccg cgtcgatacc
     7741 gtcgtttcgc tgtcacagga cccgcgagcc aagctgcaat acttccagac gatgttcgat
     7801 ttcacatgaa gactcacctc agacaggcgc gcgatgtcac gacccagcag gtcgcgtcct
     7861 ttaccgggga gggcgcgtat cacgggtcga cccgggcccg ccccgcgccc gtgcaagcgg
     7921 ccccggacgt cgtcgtcgag gagatgcggc cctgctcgct cgacaaatac gacgccagcg
     7981 agccccgcga gatcgacacc tttgatgccc acttcccggc ccgctacgag gtgctcgacg
     8041 ccctgcccgt cccgacccag gagcagatcg tcgcggacgg cgtcaccttt tgctggtgtc
     8101 cgatcgacga ggcgatccgc gaggccgggc cgctgacccg cagcgtgctg gaggcgatgc
     8161 agcgccgcgt cacgggcagc aagcggtatg tctatatcga ctcgaagatc cagtacttcg
     8221 agcacggcga cgtcccggtc gacagccagc actggcacgt cgacggatcg atcgccgtgc
     8281 gggacgaacg tgcgcgccgc ctcggtcatg cgctgctgca cgacatgcgg gcgcggctgg
     8341 agggccccgc ggcgccgccc acctacctct cctatcaatc ctcggagcac tgcgcgacgc
     8401 tgttcgtcac cgagcccgtc gcgctggtgc tgcccgagct gatcccgagc ttcgacgagc
     8461 tcgatcgtca ggtccgcgcg ctcgccccgg cgtgtgtggc ccagccggcg gcgacgatcg
     8521 cccgcttcga cggtttgtcc ctgcaccggg cggtcgccgc gtcggcggcg ggctggaggt
     8581 tgtggatccg ctgcgccgag accgaccgcc gcgtcgagct gaccgcgtcg atcatcgact
     8641 gctacggcac ggtctaccgg acctgagctg cttcagcgcg ggcccacgtg tctcacgcgc
     8701 gggcgagcat caggcagaaa agcgcgatca cgccgtggcg cacggagtac attgttcgca
     8761 gagtaccgcg agcgggtcgt cgacgggggc ctgctcgacg ggtggttcgc ctggcatact
     8821 cgcgcgatgt cgttcgacag caagctctac acccgcgacc ggccgatcct cgacgagacc
     8881 cagacgagct ggctcgcgcg cgacggcgcc tacacctggg agtacgagaa gtggctgttc
     8941 ggcgggatga tcgtcgacgt cgagccctac gagttcgtcg cgggcgacgg cgaccccctc
     9001 ggtgaagagt tccccgaggc ggacgaggtc ccggactgga cacgcgagat acgctgggtc
     9061 gtctgcgtca gcggcagccg ccccgacgac cacttcttca actggttcga cacgctcgcc
     9121 accgacgcgc gcggcgtgat ctggaccccc tacgacaagc actcgctcga gcacgtcgac
     9181 ggcttcgagc cgcgttacgt cgcctggcga atgctgctcg ccacccggcc gatccactgc
     9241 ggtcacaacg atcgcgcgcg cgacctcgtc cgccgcgacg cgaaacccga gaaggagccg
     9301 gcgctcgacg tcccggtcga cctggccgag atcgacgcgg cctggcgggc cgcgcgggcc
     9361 attgtttacg gcgccgctct ggcgaccgcg gcggacgccg gcgtgcgcgt gtcgtgggcc
     9421 gcggcgcagc acctgcacct cgccgcggac cccgacgccg acgggccgct gtggttctcg
     9481 atgttcgacg cgccggtgcg cggccgggcc gacgcgcccg cgatgctcac gctgacggcc
     9541 cacccggatc ctcgcatcga cgccctgctg cgcgacccgg ccggcgcggc cgagctgcgc
     9601 gcgctcgcca gtgatacgag cgatccgctc ctgcgggcgc tcgccggcct cgaaccggcc
     9661 gagccggcgc cgctgcccga cagtcgcgag ctacgcaacg agctggtcga attctgggag
     9721 cgcgtcgtca tgctcgaccc gcggctcgac ccggccgagc tcgacgacgt ccggcgccca
     9781 cgcatgggct gatgacgtcc ccggcggtct gccgacgtgt ataccgacgg acttcgcgcc
     9841 ggccagcgtg cgatcgtgga cggggtcgcc ggaccacgga taccgag
//
LOCUS       DASWNJ010001510         2014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_486156, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001510 DASWNJ010000000
VERSION     DASWNJ010001510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486156"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VGB85_25125"
     CDS             complement(<1..611)
                     /locus_tag="VGB85_25125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137400.1"
                     /translation="MPAACGDAGGGATDTQGQTMTSVSTINPTAASETDPSAGTTTNV
                     SATEDPTEGSMSGTNMTTPTSTTDDSTSGSTTTPVDPSTTTDPGTTTTTDGTTTGTTG
                     PAPCDEIKATLKPIEPNIMLVLDKSGSMVANPAGFWDHDADPNTPKITRWNSLYQVVQ
                     NIVTSYNDVINFGANLFPSTMAQQKYDATACVVNPNVEIPVAP"
     gene            complement(859..1344)
                     /locus_tag="VGB85_25130"
     CDS             complement(859..1344)
                     /locus_tag="VGB85_25130"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0137401.1"
                     /translation="MRPLALALALALAPLAGLGCAHAGGFTGADRDAIVALLAAQQSS
                     WNRGDLEGFLRGYERSQDLLFTSGGEVRRGYAATRAQYQARYLGMAGATGTPDRSKMG
                     QLAFTVRDVRGLGRDGAIVLGEWRLTGTPEAGEGVFSLAFFRTRAGWRIVHDHTSLAT
                     P"
     gene            1467..>2014
                     /locus_tag="VGB85_25135"
     CDS             1467..>2014
                     /locus_tag="VGB85_25135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137402.1"
                     /translation="MAASLAQRIFSRIAAIPLRRPLLVLTVSGGLVAGAAYIASGLGV
                     STSRFGLVSAENPDQARMLRFFERFGNPDAPVMLISGGSPAERQALVDEVSEKLKSDP
                     GLAGRVLARVGPDEVAEALLLQKPEALAEVAAGLPKDLPVAGLLEGGLPAWFGAVEQQ
                     IQAGLDGEAPAQDPAKAAEGLRG"
BASE COUNT          287 a          662 c          740 g          325 t
ORIGIN      
        1 ttcggggcga ccgggatctc gacgttgggg ttcacgacgc aggcggtcgc gtcgtacttc
       61 tgctgcgcca tcgtcgaggg gaacaggttg gcgccgaagt tgatcacgtc gttgtacgag
      121 gtgacgatgt tctggacgac ctggtacagg ctgttccagc gcgtgatctt cggggtgttc
      181 gggtcggcgt cgtggtccca gaagccggcc gggttggcga ccatcgagcc cgacttgtcg
      241 agcacgagca tgatgttcgg ctcgatcggc ttgagcgtcg ccttgatctc gtcacacggg
      301 gccggaccgg tggtgccggt ggtggtgcca tcggtggtcg tcgtcgtgcc cgggtcggtg
      361 gtcgtcgacg ggtcgacggg cgtcgtcgtc gaaccggagg tcgaatcgtc ggtggtcgac
      421 gtcggcgtcg tcatgttggt ccccgacatc gaaccctcgg tcggatcctc ggtcgcgctg
      481 acgttggtgg tcgtgccggc gctcggatcg gtctcgctgg cggccgtcgg gttgatcgtc
      541 gacacggagg tcatcgtttg accctgcgtg tcggtggcgc cgccgccagc gtcaccgcaa
      601 gcggccggca tgaaggaaat agtaaggatc agggaacccg acgcgattcg cattgagaca
      661 ctccgttaag tccaggtttg aaaaacagga cgcccgacta tacagcaccg tcgctggacc
      721 ggctgcgaaa aattcggggg ccgcagcgtg ctgcccgtga cgtaggggcc ggcggctcac
      781 gcggcgcgcc tgcgctgcga ccctgcacga gccggtcctg acactccatg cgacgcgctg
      841 cgggccgcgg acacatgctc acggcgtcgc cagcgaggtg tgatcgtgga cgatgcgcca
      901 gcctgcgcgc gtgcgaaaaa atgccaggct gaagaccccc tcgcccgcct ccggtgtgcc
      961 cgtcaagcgc cactcgccga gcacgatggc gccgtcgcgg ccgagcccgc ggacgtctcg
     1021 caccgtgaac gcgagctgcc ccatcttgct acggtccgga gtccccgtgg cgcccgccat
     1081 cccgaggtag cgggcctggt attgggcccg cgtggccgcg tatccgcgcc gcacctcgcc
     1141 gccggaggtg aacaggagat cctgcgagcg ctcgtagccg cgcaaaaatc cctcgagatc
     1201 gccgcggttc cacgagctct gctgcgcggc caacaacgcg acgatcgcgt cccggtcggc
     1261 gcccgtgaac ccgccggcat gggcgcatcc gaggccggcg aggggggcga gggcgagggc
     1321 gagggcgagg gcgagggggc gcatgaccgg gattgtaccg ccctgagcgc ggtacaacgg
     1381 ccatttaacc cagcggcgga tcacgcgcga agaccggcct cgcggctgcg gtgcgcggcg
     1441 caccctggta tagctaggcc ctcgcgatgg ctgcgtcact cgctcaacgg atcttctcca
     1501 ggatcgccgc gatcccgctt cggcgccccc tcctggtcct cacggtgtcg ggcgggctcg
     1561 tcgccggcgc ggcgtacatc gcctcggggc tgggcgtcag cacctcgcgc ttcgggctgg
     1621 tgtccgcgga gaacccggac caggcccgca tgctccgctt cttcgagcgc ttcgggaatc
     1681 cggacgcgcc ggtgatgttg atctccggcg gttcacccgc cgagcgccag gcgctcgtcg
     1741 acgaggtgag cgagaagctc aagagcgacc ccggcctcgc gggtcgcgtg ctggcgcggg
     1801 tcgggcccga cgaggtcgcc gaggcgctgc tgctgcaaaa accggaggcg ctggccgagg
     1861 tcgcggccgg attaccgaag gatctgccgg tcgcgggcct cctcgaagga ggtttgccgg
     1921 cgtggttcgg cgcggtcgag caacagatcc aggccgggct cgacggcgag gccccggcgc
     1981 aggacccggc gaaggccgcc gagggcctgc gtgg
//
LOCUS       DASWNJ010001511         3575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_696486, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001511 DASWNJ010000000
VERSION     DASWNJ010001511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696486"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VGB85_25140"
     CDS             <1..291
                     /locus_tag="VGB85_25140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137403.1"
                     /translation="LYEHCLEPTPCDPQTDACFTVNDANNNPTDGYCTILCNTVADCG
                     TAPKAPAVQECFALNANQKLCGLKCKGVADCPTGMACINLALPNMQTGMYCF"
     gene            364..2706
                     /locus_tag="VGB85_25145"
     CDS             364..2706
                     /locus_tag="VGB85_25145"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY0137404.1"
                     /translation="MTLEDPTLAGEPVIDLAIAGMRCANCAGTIERALAAVAGVEAAA
                     VNFASETASVRLHAPSTVSRGALVAVIEAAGFGVIEGSEGAGDEAEVAARAQELAAAR
                     RRVAVGIACTLPLLVLGMGRDFALLGAWAAAPWVGWLMLALALPVLAYTGAPHLRGAY
                     HALRAGAANMDVLICLGAGVAFLASVPVVIADSLGSHTLGHHLYFETAAAIVALVGLG
                     RLLETRARGQAGAAIRALLALRPATARVRRDGQELEVPSASVRVGELVVVRPGEAIAV
                     DGLVVAGASEVDESMLTGESRPVARTIGDPVTGATINGDGALEVKATRVGSATALAQI
                     VRLVRAAQGSKAPVQRLVDRVAAVFVPVVLAIAAITFVVWSLVGPGPAEALMRAVAVL
                     VIACPCALGLATPTAIVVGTGAAARRGILFKNMTALERVHALRTIVLDKTGTLTRGRP
                     VLGAVVPAPGGDPTELLRLAAAAEQHSEHPLGKAILAAAQAVVGPLPAVTQHRAVGGH
                     GLRATVEGAVVLAGTERWLRSNAVDTTPLATTAAGLSSGTVSEGTGLQILPGATLAVP
                     QTLVWVARDGVLLGVLALADALRPEAPAAVAALRRAGLRVVVLTGDRREVAVAIAAEA
                     GIAADDVMAEVLPGDKAAAVASLQAQGLVGMVGDGINDAPALARADVGIAVGSGADVA
                     IHAADVTLMRSDLGGLAEALALSRRTVAVIRQNLVWASIYNLVLIPVAAGVLYPIAAA
                     PAALRSLHPALAALAMALSSLTVVLNSLRLRRLAEPRGGS"
     gene            complement(2657..3199)
                     /locus_tag="VGB85_25150"
     CDS             complement(2657..3199)
                     /locus_tag="VGB85_25150"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY0137405.1"
                     /translation="MPRPVHILSLLHDDDIDWLAQVGLRLNLAQMTPLVEEGIRNPFL
                     YIILSGAFIVTSSVAGVTGERPISILQRGELVGELSFLDARPPSATVRAARPAQVLAV
                     SRAEVMRKLSADLGFAARFYRALGITVAMRLRTQTHGQEAADEELDLEQLGPVTVDEG
                     RFEAIMNRLEAPQADAGAAS"
     gene            3305..>3575
                     /locus_tag="VGB85_25155"
     CDS             3305..>3575
                     /locus_tag="VGB85_25155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137406.1"
                     /translation="MRSPALALTALLACSRPASPEAPAKQPAPPAIFELEARREAATP
                     DLIARTREAGDEALRAIRALGRVGDAPAIARLLELLAAPEPPRREA"
BASE COUNT          434 a         1371 c         1306 g          464 t
ORIGIN      
        1 ctgtacgagc actgcctcga gccgaccccg tgcgatccgc agaccgacgc ctgcttcacg
       61 gtcaacgacg ccaacaacaa cccgaccgat ggttactgca cgatcctgtg caacaccgtg
      121 gccgactgcg gcaccgcgcc caaggccccc gcggtccagg agtgcttcgc gctcaacgcc
      181 aatcagaagc tgtgcggcct caagtgcaag ggcgtcgccg actgcccgac cggcatggcc
      241 tgcatcaacc tcgccctgcc gaacatgcaa accggcatgt actgcttctg accggcggcc
      301 cgcgtgagga cgggcgcgtg gggcgacgtg tggcggccgc ggcgcgccgg ctatgctggg
      361 agcgtgacgc tcgaggaccc gacgctcgcg ggtgagcccg tgatcgacct ggcgatcgca
      421 ggcatgcggt gcgccaactg tgccggcacg atcgagcgcg cgctcgcggc cgtcgccggg
      481 gtcgaggcgg ccgccgtcaa cttcgccagc gagaccgcca gcgtgcgcct gcacgcgccg
      541 agcacggtca gccgcggggc cctcgtcgcc gtgatcgagg cggcaggctt cggcgtgatc
      601 gagggcagcg agggcgccgg tgacgaggcc gaggtcgccg cgcgggcgca ggagctggcc
      661 gcggcgcgcc ggcgcgtcgc cgtcgggatc gcgtgcacgc tgcccctgct ggtgctcggc
      721 atgggccgcg acttcgcgct gctcggcgcc tgggccgccg cgccgtgggt cggctggctg
      781 atgctggcgc tcgcgctgcc agtgctcgcc tacaccggcg cgccgcacct gcgcggggcc
      841 taccacgccc tgcgggccgg cgcggccaac atggacgtcc tgatctgcct cggcgccggc
      901 gtggccttcc tcgccagcgt gcccgtcgtg atcgccgaca gcctcggcag tcacacgctc
      961 gggcatcacc tgtacttcga gacagccgcg gcgatcgtcg cgctggtcgg cctcgggcgc
     1021 ctgctggaga cccgcgcgcg cggccaggcc ggcgccgcga tccgggcgct gctcgccctg
     1081 cgtccggcga ccgcgcgcgt gcgccgggac ggccaggagc tcgaggtccc gtcggcgagc
     1141 gtgcgcgtcg gcgagctcgt cgtcgtgcga ccgggggagg cgatcgcggt cgacggcctg
     1201 gtggtcgcgg gcgcctcgga ggtcgacgag agcatgctga ccggcgagag ccgcccggtc
     1261 gccaggacca tcggcgaccc ggtcaccggc gcgacgatca acggcgacgg cgcgctcgag
     1321 gtcaaggcga cccgcgtcgg ctcggcgacg gcgctggcgc agatcgtccg cctcgtgcgc
     1381 gcggcgcagg gcagcaaggc gccggtgcag cgcctcgtcg accgcgtcgc cgccgtgttc
     1441 gtgccggtcg tgctggcgat cgcggcgatc accttcgtgg tgtggtcgct ggtcggcccg
     1501 ggccccgcgg aggcgctgat gcgggccgtc gcggtgctgg tgatcgcgtg cccgtgcgcg
     1561 ctcggcctgg cgaccccgac ggcgatcgtc gtcggcaccg gcgccgccgc ccgccgcgga
     1621 attctattca aaaatatgac ggcgctcgag cgggtccacg cgctgcgcac gatcgtgctc
     1681 gacaagaccg gcaccctgac gcgcggccga ccggtcctcg gggcggtggt cccggccccc
     1741 ggcggcgatc cgaccgagtt attgcgcctc gccgccgccg ccgagcaaca cagcgagcac
     1801 ccgctcggca aggcgatcct cgccgcggca caagccgtgg tgggacccct gcccgcggtc
     1861 acccagcacc gcgccgtcgg cgggcacggc ctgcgcgcga ccgtcgaggg cgccgtcgtg
     1921 ctcgccggca ctgaacgctg gctgcgcagc aacgccgtcg acaccacccc gctcgccacg
     1981 accgcggcgg ggctgtcctc cgggacagtg tccgaaggga caggtctaca gatcctgccc
     2041 ggggccacgc tcgcggtccc gcagacgctc gtgtgggtgg cccgcgacgg cgtgttgctc
     2101 ggcgtgctcg cgctcgccga cgccctgcgc ccggaggccc ccgccgcggt cgcggccctg
     2161 cggcgcgccg gcctgcgcgt ggtcgtgttg accggcgacc ggcgcgaggt cgcggtcgcg
     2221 atcgcggccg aggccggcat cgccgcggac gatgtcatgg ccgaggtgct gcccggcgac
     2281 aaggcggccg cggtcgccag cttgcaggcg cagggcctcg tcggcatggt cggcgacggc
     2341 atcaacgacg cgcccgcgct ggcccgcgcc gacgtcggga tcgccgtcgg cagcggtgcc
     2401 gatgtggcga tccacgccgc cgacgtcacg ctgatgcgca gcgacctcgg cgggctcgcg
     2461 gaggcgctgg cgctgtcgcg ccgcacggtc gccgtcatcc gccagaacct ggtctgggcg
     2521 tcgatctaca acctcgtgct gatcccggtc gccgccgggg tcctgtaccc gatcgccgcc
     2581 gcaccggcgg ccctgcgctc gctgcacccc gcgctcgccg ccctggcgat ggcgctcagc
     2641 agcctgaccg tcgtgctcaa ctcgctgcgc ctgcgtcggc ttgcggagcc tcgaggcggt
     2701 tcatgatcgc ctcgaagcgg ccctcgtcga ccgtcaccgg cccgagctgc tcgaggtcta
     2761 gctcctcgtc ggcggcctcc tgcccgtgcg tctgggtgcg caggcgcatc gccaccgtga
     2821 tgccgagcgc gcggtagaag cgggccgcaa agccgaggtc ggccgagagc ttgcgcatca
     2881 cctccgcgcg agacaccgcg agcacctgcg ccggcctcgc cgcccgcacg gtcgccgacg
     2941 gcggccgggc gtcgaggaac gacagctcac cgacgagctc gccgcgctgc aggatcgaga
     3001 tcgggcgctc cccggtgacc ccggcgaccg agctggtgac gatgaaggcg ccgctgagaa
     3061 tgatgtacag gaacgggttg cggatcccct cctccaccag cggcgtcatc tgggccaggt
     3121 tgaggcgcag accgacctgc gcgagccagt cgatgtcgtc gtcgtgcagc aacgacagga
     3181 tgtgcaccgg cctcggcatc gccgcgaacg tagcaggcgg gcccccgcgt cgccacccgg
     3241 tcccgcgtgt cgtcgcggac ccccggaaca cgacgttccc ggggtggcgt acactcggcc
     3301 ccgcatgcga tcgccggccc tcgccctcac ggccctgctc gcctgctctc gccccgcgag
     3361 ccccgaggcg cccgcgaagc agccggcacc gcctgcgatc ttcgagctcg aggcgcgccg
     3421 cgaggccgcg accccggacc tgatcgcgcg cacccgcgag gccggtgacg aggccctgcg
     3481 cgcgatccgg gccctcggtc gcgtcggcga tgcacccgcg atcgcccgct tgctcgagct
     3541 gctcgccgcc ccggaaccgc cgcgccgcga ggccg
//
LOCUS       DASWNJ010001512         4933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_204073, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001512 DASWNJ010000000
VERSION     DASWNJ010001512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204073"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VGB85_25160"
     CDS             <1..791
                     /locus_tag="VGB85_25160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0137407.1"
                     /translation="GGIMLPNHAPYIIAEQFGTLETLFPGRIDLGLGRAPGTDQSTLR
                     ALRRDPAGADHFPQDVQELQAFLGPVRPGQRIEAVPGSGTQVPLWILGSSLFGAQLAA
                     SLGLPYGFASHFAPQALDQALAIYRHQFRPSAQQDMPYALVGVNVIAAESDGEARRLA
                     TSQQMSFADLFRGTRRLTQPPIEDIETYWTPQERFQASQMLACSVIGGPETVRAGLRA
                     LVERTKADELMIVSDIYEPAKRFKSFEIIAEVARGMPRLAGTSA"
     gene            complement(800..1444)
                     /locus_tag="VGB85_25165"
     CDS             complement(800..1444)
                     /locus_tag="VGB85_25165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137408.1"
                     /translation="MTTITAGGPVSSSVRVCLYHAAVLALSLVCTLLAAPPEAPDPFD
                     PAASRPLSPSEQARQERRAINLRIEARAQQLATRQGWGVKEEWDDFGDGRFFDHAVTD
                     SANNLSSGRVMLGIGGALSALGVGLLIAGGLDGGVDGLPLMIGGGLALVPGVSLTLPG
                     AIRVGIHRGRLDDLYRIEREERRAGATARLRFRGLAPLYVPALGTGGLTLRLAF"
     gene            complement(1557..3869)
                     /locus_tag="VGB85_25170"
     CDS             complement(1557..3869)
                     /locus_tag="VGB85_25170"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEY0137409.1"
                     /translation="MSTPSVWTVTGLLDYFQEVEDTPDRRFAFILGAGASVQSGIPMA
                     GHLVDSWLRELHAREDHERRPLAQWANAATLGIPGFVYDRAVESYSQVFARRFAGRPD
                     EGHAYLERILHGRDPSFGYSVLAQILANTRHRVVITTNFDNLVADALAIYTNQLPFVC
                     GHESLAGFVRPNPRRPLVVKIHRDLLLAPKNTSDEIAALPDPLVSSLRGLLAGFTPIV
                     VGYGGNDGSLMGLLRETLRPGDIPGGIYWCYWAGGGLPGPAITEVVQRHGGALIPIAG
                     FDELMVQLNHRLGYPRMDQRIEQRARERAQRYRQSFEALHQRVLQTEVALAEDDFEVD
                     AADEAEAEEASAAVPADMSDVASPTPSRARVSPSQGPSPPSAPPGGSPHTGSPATRGA
                     APGEPVLSGRPGPRDAAPDAAVFSGRPGSRDAAPDAPVPSSRSVTRSGEPRDGPAVTP
                     PVSPASAPRRAPQSTTVGASPVTRAAASTPDIEADDFAPKQARASLARPAPPPVLESA
                     SLASTTAPASFAGGIETSAGPDEPKDRADLPRREPPDAATAALQQAVQALVGDSPAEL
                     DWWTLKLHADQQPTPATRIAAYREAVARFPDNAALLRGLACQLALANVRGEARELYWR
                     LEQWTPDDPQIHLDLAVLFFRWGAGKTRSEPYASTAWHLARAQGQDPSMLAAVALVRG
                     LLACLHMRDDTTALRVLRGLLPVVAGPLQDFAGVLETAARKHLDMPTYRLYHRVLLCL
                     HGLADVQMLKTIDRWNALTPLAPEAAWPDE"
     gene            4136..4858
                     /locus_tag="VGB85_25175"
     CDS             4136..4858
                     /locus_tag="VGB85_25175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137410.1"
                     /translation="MENTLKLSGCGIHATLLALSLLGGCNSDELRLESASDSQGVTTD
                     SATGPTTGSASMTATETGPTTGGTMSGTMSDSEAGSGSVGESHSTGMSASTTVTATDV
                     TAASATDSTSTTDGTSATDSETLGTVSDTEVTVSDTDVTLTGTTDTGDTDTGEVCSPD
                     VPDDNACEQCMAANCCEQYAACVADNACSCLVDACSAPNAVPVVCALPCALNPQKLMM
                     ALAYLMGCSGPCGAQCAALGLL"
BASE COUNT          699 a         1638 c         1944 g          652 t
ORIGIN      
        1 ccggcggcat catgctgccc aaccacgcgc cgtacatcat cgccgagcag ttcggcaccc
       61 tggagacgct gttccccggc cgcatcgacc tcggcctcgg gcgcgccccg ggcaccgatc
      121 agtcgacgct gcgcgcgctc cggcgtgacc ccgccggggc cgaccacttc ccgcaggacg
      181 tccaggagct gcaggcgttc ctcggcccgg tgcgcccggg gcagaggatc gaggcggtcc
      241 ctggatcggg cacgcaggtg ccgctgtgga tcctcggctc cagcctgttc ggcgcgcagc
      301 tggccgcgag cctcgggctg ccctacggct tcgcctcgca cttcgcaccg caggcgctcg
      361 accaggcgct ggcgatctat cgccaccagt tccggccgtc cgcgcagcag gacatgccgt
      421 acgcgctcgt cggcgtcaac gtgatcgcgg cggagagcga cggcgaggcg cgccgcctgg
      481 cgacctcgca gcagatgtcg ttcgccgacc tgttccgcgg cacccgccgc ctgacgcagc
      541 cgccgatcga ggacatcgag acctactgga ccccgcagga gcggttccag gcctcgcaga
      601 tgctggcgtg cagcgtgatc ggcgggccgg agacggtgcg cgccgggctg cgcgcgctcg
      661 tcgagcgcac gaaggcggac gagctcatga tcgtgtcgga catctacgag cccgcgaagc
      721 gcttcaagtc gttcgagatc atcgccgagg tcgcgcgcgg gatgccgcgg ctcgccggga
      781 cgtcggcctg agacggcgct cagaaggcga ggcgcagcgt cagcccgccg gtgccgagcg
      841 cggggacgta cagcggcgcg agcccgcgaa agcgcagcct cgcggtcgcc cctgcgcgcc
      901 gctcctcgcg ctcgatgcgg tagaggtcgt cgaggcggcc gcgatggatg ccgacgcgga
      961 tcgcccctgg cagcgtgagc gagacccccg ggaccagcgc gagcccacca ccgatcatca
     1021 gcggcagacc gtcgacgccg ccgtcgaggc cgccggcgat caacaagccg acgccgaggg
     1081 cagacagggc gccgccgatg ccgagcatga cccgcccgct cgacaggttg ttggcgctgt
     1141 cggtgaccgc gtggtcgaag aagcggccgt ccccgaagtc gtcccactct tctttcacgc
     1201 cccagccctg gcgcgtggcc agctgctgcg cgcgcgcctc gatgcgcagg ttgatcgcgc
     1261 gccgctcctg acgcgcctgc tcgctcggcg acaggggccg cgaggcggcc gggtcgaagg
     1321 gatccggcgc ctcgggcggt gcggcgagca gggtgcacac gagcgacagg gcgagcaccg
     1381 cggcatggta caggcacacg cgcacggacg aggataccgg cccgcccgcg gtgatcgtgg
     1441 tcaccgcgag gtgcagcggc gcgcgcctcg acagcgagct cgtgccactg ggggcgcgac
     1501 ctcggccgac ctggagaccg ggccggtcgg tcgggcgagc tgtccccggg gacagctcat
     1561 tcgtcgggcc acgcggcctc gggcgcgagc ggggtcaggg cgttccagcg gtcgatcgtc
     1621 ttgagcatct gtacgtccgc gaggccgtgc aggcacagca ggacgcggtg gtacaggcga
     1681 taggtgggca tgtcgaggtg cttgcgagcc gccgtctcga gcacgcctgc gaagtcctgc
     1741 agggggccgg cgacgaccgg cagcaggccg cgcaggaccc ggagcgcggt ggtgtcgtcg
     1801 cgcatgtgca ggcacgcgag caggccgcgg accagcgcga ccgcggcgag catcgagggg
     1861 tcctggccct gcgcgcgggc caggtgccag gcggtgctcg cgtacggctc ggaccgggtc
     1921 ttgcccgcgc cccagcggaa gaacaggacg gcgagatcga ggtggatctg cgggtcgtcg
     1981 ggggtccatt gctcgagccg ccagtacagc tcgcgcgcct cgccgcggac gttggcgagc
     2041 gcgagctgac aggcgagccc gcgcagcagc gccgcgttgt cggggaagcg cgcgacggcc
     2101 tcgcggtagg ccgcgatgcg cgtcgccggg gtcggctgtt gatccgcgtg cagcttgagg
     2161 gtccaccagt cgagctcggc ggggctgtca ccgacgagcg cctgcacggc ctgctgcagc
     2221 gccgcggtgg ccgcgtcagg gggctcgcgt cgcgggaggt cggcgcggtc ctttggttcg
     2281 tcgggtccgg ccgaggtctc gatcccgccg gcaaagctcg cgggtgcggt ggtcgacgcg
     2341 agcgatgcgc tctcgagcac cggtggcggc gcgggcctgg cgagcgatgc tctcgcttgc
     2401 ttcggagcga agtcgtcggc ttcgatatcg ggtgtgctgg ccgcggcgcg ggtcaccggg
     2461 ctggcgccga cggtcgtgct ctgcggggcg cgccgtggcg cgctcgcggg cgagacggga
     2521 ggcgtgaccg cggggccgtc gcgaggttcg ccgctgcggg tcaccgacct gctcgaaggg
     2581 acaggtgcat cgggggccgc gtcgcgtgat ccgggcctgc ccgaaaacac agctgcatcg
     2641 ggggccgcgt cgcgtgggcc cggcctgccc gaaagcacag gttctccggg tgccgcgccg
     2701 cgggtcgccg ggctgccggt gtgtggggat ccgccgggcg gggccgaggg cggggacggg
     2761 ccctgggacg gcgatacgcg ggcccgcgag ggcgtcgggc tggcgacatc ggacatgtcc
     2821 gcggggacag ccgcgctcgc ctcctcggcc tccgcctcgt cggcggcgtc gacctcgaag
     2881 tcgtcctcgg cgagcgcgac ctcggtctgc agcacgcgct ggtgcagggc ctcgaagctc
     2941 tggcggtagc gctgggcgcg ttcgcgggcg cgctgctcga tgcgctggtc catgcgcggg
     3001 tagccgaggc ggtggttgag ctggaccatc agctcgtcga agccggcgat cgggatcagg
     3061 gcgccgccgt ggcgctgcac gacctccgtg atcgccgggc cgggcaaacc gccgccggcc
     3121 cagtagcacc agtagatgcc gcccgggatg tcgccggggc gcagggtctc gcgcagcagg
     3181 cccatcaggc tgccgtcgtt gccgccgtag ccgacgacga tcggcgtgaa gccggcgagc
     3241 aggccgcgca gcgaggacac gagcgggtcg ggcagcgcgg cgatctcgtc gctggtgttc
     3301 ttgggggcga gcagcaggtc gcggtggatc ttgacgacga gcgggcggcg cgggttgggg
     3361 cggacaaagc cggcgagcga ctcgtggccg cacacgaagg gcagctggtt ggtgtagatc
     3421 gccagcgcgt cggcgacgag gttgtcgaag ttggtggtga tgacgacgcg gtggcgtgtg
     3481 ttggcgagga tctgcgcgag caccgagtag ccgaagctcg ggtcgcggcc gtggaggatg
     3541 cgctcgaggt aggcgtggcc ctcgtcgggg cggccggcga agcggcgcgc gaacacctgc
     3601 gagtacgact cgacggcgcg atcgtacacg aagccgggga tgccgagagt ggcggcattg
     3661 gcccactgcg cgagcggacg gcgctcgtga tcctcgcggg cgtgcagctc acgcagccag
     3721 ctgtcgacga ggtggccggc catcgggatg ccggactgca cggacgcgcc ggcgccgagg
     3781 atgaaggcga agcggcgatc gggggtgtcc tcgacctcct ggaagtaatc cagcaggcca
     3841 gtgacggtcc agacggacgg ggtggacatg ccgggcttcg atggtgtggt cgcggggtcg
     3901 aggagcgcaa ggtgggacat ggggccggcg ccggagtgcc cggccggcct gggacaattt
     3961 actggcacga ggtctgcacc gcgacagtgc agatgtccgg tcggaccgtc acggtgcagc
     4021 tcgggatcgc cggaggaccg gcgaaattgc cgtgctacgg cgttacgggc cgatccccgg
     4081 ggggtcccgg cgcgcgggtt gccggatcga cgaacccggc ccagacaggt gtgtcatgga
     4141 aaatactttg aaattaagcg gctgtgggat ccatgccacg ctgctggccc tgagtttgtt
     4201 gggcggctgt aacagcgacg agctccggct cgagagcgcc tccgacagcc agggtgtgac
     4261 gaccgattcc gcgaccgggc cgacgacggg ctcggcctcg atgacggcga ccgagaccgg
     4321 gccgacgacc ggcgggacga tgagcgggac gatgagcgac agcgaggccg gcagcggcag
     4381 cgtcggcgag tcgcactcga ccggcatgag cgcgtcgacg acggtgaccg cgaccgacgt
     4441 gacggccgcg agcgcgaccg acagcacgag cacgaccgac ggcacgagcg cgaccgacag
     4501 cgagacgctc ggcaccgtga gcgacacgga ggtgaccgtg agcgacacgg acgtgacgct
     4561 gaccggcacg acggacacgg gcgacaccga cacgggcgag gtgtgcagcc cggacgtgcc
     4621 cgacgacaac gcgtgcgagc agtgcatggc ggccaactgc tgcgagcagt acgcggcgtg
     4681 cgtggcggac aacgcgtgct cgtgcctggt cgacgcctgc tccgcgccca acgcggtgcc
     4741 cgtggtctgc gcactgccct gcgccctcaa cccgcagaag ttgatgatgg cgctcgcgta
     4801 cctgatgggc tgctcggggc cgtgtggagc gcagtgtgcc gcgctggggc tgctgtgacg
     4861 gccggccacg tgttatgcag agccgtcgcc ccaggggaac agacatgagc atcgtgcagc
     4921 gttcgtgggt gtg
//
LOCUS       DASWNJ010001513         1525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_378458, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001513 DASWNJ010000000
VERSION     DASWNJ010001513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378458"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="VGB85_25180"
     CDS             <1..1250
                     /locus_tag="VGB85_25180"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0137411.1"
                     /translation="ARPTEGLLWARHAEALLDRTGREPDATASYHNARANIAVRTGDY
                     AQAEVSYRAALAAHELEQPRDELAIARVLNNLASTIGNLGKLPEALELLQRALEIRTA
                     NLGPDHPDIAFVLSTIGGSHALLGDAAAAEQAQRRALAIWRAALPPGHERLAYPLSAL
                     GDIALERARYEEARAHYEEAHTIWRSHYGPDHPQVLSMQERLAGVMFTQGDLTRARAS
                     ADEALEIWDSIAQPEPGGFTLILLARAQIAVIDGDHAKATALMQRALDTAVAAFGEGH
                     LYAASTRMLMLGHKIDQGRAAEALPGLVALVAEFAGQYGDDARWLIEPLLTLGRARHA
                     VKDYAAARADLERARKLATVHGIVGQFGDLDLALARVLWDSDADRPGARTLAQAAVEV
                     HRRSGAARSLAEAETWLKEHPLP"
     gene            1247..>1525
                     /locus_tag="VGB85_25185"
     CDS             1247..>1525
                     /locus_tag="VGB85_25185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137412.1"
                     /translation="MNATTRAAAARRRAAIVALVLVGLGVLVIAAQPPIRQPQDYHDF
                     ADHRALLGVPNGGDVLSNVGFVIAGLAGLLACARRSFAARPQYLVFFAG"
BASE COUNT          206 a          623 c          506 g          190 t
ORIGIN      
        1 tcgcgcggcc gaccgagggc ctgctgtggg cccgccacgc cgaggcgctg ctcgaccgca
       61 ccggccgcga accggacgcc accgccagct atcacaacgc ccgggccaac atcgccgtgc
      121 gcaccggcga ctacgcgcag gcggaggtct cgtatcgcgc cgcgctcgcg gcccacgagc
      181 tcgagcagcc gcgcgacgag ctggcgatcg cccgcgtgct caacaacctc gcgagcacga
      241 tcggcaacct cggcaagctg cccgaggcgc tcgagctgct gcagcgggcc ctggagatcc
      301 gcaccgccaa cctcgggccc gaccaccccg acatcgcctt cgtgctcagc acgatcggcg
      361 gcagccatgc gctgctcggc gacgccgcgg cggccgagca ggcccagcgc cgggccctgg
      421 cgatctggcg cgccgccctg ccgccgggcc acgagcggct cgcctatccg ctgagcgccc
      481 tcggggacat cgcgctcgag cgcgcccgct acgaggaggc ccgcgcccac tacgaggagg
      541 cgcacacgat ctggcgcagt cactacggcc ccgatcaccc gcaggtgctc agcatgcagg
      601 agcgtctcgc cggcgtgatg ttcacgcaag gcgacctgac ccgcgcgcgc gcctccgccg
      661 acgaggccct cgagatctgg gacagcatcg cccagcccga gcccggcggc ttcacgctga
      721 tcttgctggc ccgcgcccag atcgccgtca tcgacggtga ccacgcgaag gcgaccgcgc
      781 tgatgcagcg cgccctcgac accgccgtcg ccgcgttcgg cgagggccac ctctacgccg
      841 ccagcacgcg catgctgatg ctcggccaca agatcgatca gggccgcgcc gccgaggcgt
      901 tgcccggcct cgtcgcgctg gtggccgagt tcgcgggcca gtatggcgac gacgcccggt
      961 ggctgatcga accactgctg accctcggtc gcgcccgcca cgccgtcaag gattacgcgg
     1021 ccgcccgcgc cgacctcgag cgcgcgcgca agctggcgac cgtccacggg atcgtcggcc
     1081 agttcggcga cctcgacctc gccctcgcgc gcgtcctgtg ggacagcgac gccgaccgcc
     1141 ccggcgcccg aaccctcgcg caggccgccg tcgaggttca tcgccggtcc ggcgccgccc
     1201 gctcgctcgc cgaggccgag acctggctca aggagcaccc gctgccatga atgcgacgac
     1261 acgggcggcc gcggctcgcc ggcgcgccgc gatcgtcgcg ctcgtgctcg tcggtctcgg
     1321 cgtgctcgtg atcgcggcgc agccaccgat ccgccagccg caggactatc acgacttcgc
     1381 cgaccaccgc gcgctgctcg gcgtgcccaa cggcggcgac gtactgtcca acgtcggctt
     1441 cgtgatcgcg ggcctcgctg gcctgctcgc ctgtgcgcgc cgcagcttcg ctgctcgccc
     1501 gcaatacctc gtgttcttcg ccggc
//
LOCUS       DASWNJ010001514         2965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_131320, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001514 DASWNJ010000000
VERSION     DASWNJ010001514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131320"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VGB85_25190"
     CDS             complement(<1..611)
                     /locus_tag="VGB85_25190"
                     /inference="COORDINATES: protein motif:HMM:NF017315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HEY0137413.1"
                     /translation="MHSCPKVDPGPVPHVGGPVFAGSANVLIGNLPAARVGDAMVCFP
                     LGPADSIKAGSATVFINDCKAARRTDPGSHISGDLIVQGCPTVLIGDTTQSQPMQDAA
                     ARGTMFCEECAREEGGPANNPAETAAPAAETDTVTLTDGPPLAGDRDALTNLGVSREE
                     LAMQPDEGDELDDVRRAARQAVAFQFYADHAGPTVNPARIWSHV"
     gene            995..>2965
                     /gene="tssI"
                     /locus_tag="VGB85_25195"
     CDS             995..>2965
                     /gene="tssI"
                     /locus_tag="VGB85_25195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HEY0137414.1"
                     /translation="MISALTGNVSLFTFQAGGGLLDVVRFAGSEGVSSLFEFRVELAG
                     QDIELSALVDQPALLTIGGIEEPRHIHGHICHAEATGQTRNLHTYVVTLVPQLWRLRL
                     RQDSRIFQKKTTPQILEEVLTKAGIGKANLRFDLTSSYEPRDFCVQYRETDFDFVSRL
                     MEEDGIFYYFEHSAERHVLVMSDNPVAPPIGGAPEIWYSPPQGYVRDREHITRFTVSE
                     GIRTGKVTLRDHNLHKPDAPMESSESAAQAADLEVYDYPGEYQDNSSGTTSNRGPRLA
                     KIRLAAAQSDRRRGLGGSDCPRLTSGHAWTLTGHPRGDVDGEYKLVRVDHRGEQPQAL
                     IQDAAGGFSYSNEFVCIDAKVAFRPPRATPRPTVRGVQTATVVGPPNEEIYVDEHARV
                     KVQFHWDRTDRFDEESSCWVRVSQLWAGNTWGAMFIPRIGHEVVVDFIEGDPDRPIII
                     GRIYHGFNTPPYPLPEQKTKSTIKSDTYQGAGYNELRFEDQRDVEQVFLHAQRDLDIR
                     VLHDRMESVLHNRHLTVGSSDGGSKIGDFNENILRDHHLQIDRNRNEHIGGDLRVRVG
                     GIDGVGHVDTVIEGNRREHVQQNQSLEITGNLKVKIDGSSHEQVQGDVHRKVLGVETV
                     EALREVHLKSTKIVIEASQGLTIRGPGGFITIH"
BASE COUNT          490 a         1036 c         1018 g          421 t
ORIGIN      
        1 acgtgcgacc agatcctcgc cgggttgacc gtcgggccgg cgtgatcggc atagaactgg
       61 aacgcgacgg cctggcgagc ggcccggcgc acgtcgtcga gctcgtcacc ctcgtcgggc
      121 tgcatcgcca gctcctcgcg gctgacgccg aggttcgtca gcgcgtcgcg gtcgcccgcg
      181 agcggcgggc cgtcggtgag cgtgacggtg tcggtctcgg ccgcgggcgc ggcggtctcg
      241 gccgggttgt tcgcggggcc accctcctcg cgcgcacact cctcgcagaa catcgtgccg
      301 cgggcggccg cgtcctgcat cggctggctc tgcgtcgtat cgccgatcag caccgtcggg
      361 cagccctgga cgatcaggtc gccgctgatg tgcgagccgg gatcggtcct gcgggcggcc
      421 ttgcagtcgt tgatgaacac cgtcgccgag ccggccttga tgctgtccgc gggcccgagc
      481 gggaagcaga ccatcgcgtc accgacgcgc gccgccggca gattaccgat cagcacgttg
      541 gcactgccgg cgaacaccgg tccccccacg tgcgggacgg gccccgggtc gaccttcggg
      601 cagctgtgca tatcgctgat acgtgcggcc tcgggcatac ttcgctcgcg ctcccgcggg
      661 cgggaggtct ttccaacgta tcagagccgc gagcgcgaag gcgccaggtg gcccttcgtg
      721 gtcgccggcc cgcggcccgg ggcggagcgt gcgcttcgcc atgacgacat gtcgacatcg
      781 tgacatcgcg acacgcgcgc gggcaatctt gcgcggctcg acatcgacgc ccctgtctcg
      841 atcgtcttcg cgcgtatgca cgggtatgca cgcaccgaca cggggatgca cgcgcatggt
      901 ccagcgccgc agcggatcgc gccaggttgg caggcgatgg atggctgata cagtgctcgg
      961 gttccggagg ggtggcaggc ggatgatcgg aaacttgatc tcggcgttga cggggaatgt
     1021 ctccctcttc acttttcagg cgggaggcgg cctcctggat gtcgttcgct tcgctggcag
     1081 cgagggggtc tcctcgctgt tcgagtttcg cgtcgagctc gcgggccagg acatcgagct
     1141 gtcggcgctc gtcgaccagc cggcgttgct gacgatcggc ggcatcgagg agccgcggca
     1201 catccacggc cacatctgcc acgccgaggc gaccggccag acgcgcaacc tccacaccta
     1261 cgtggtcacg ctcgtgccgc agctgtggcg cctgcggctg cgccaggaca gccgcatctt
     1321 ccagaagaag acgacgccgc agatcctcga ggaggtgctg accaaggctg gcatcggcaa
     1381 ggccaacctg cgcttcgacc tgaccagcag ctacgagccg cgcgacttct gcgtccagta
     1441 ccgcgagacc gacttcgact tcgtcagccg cctgatggag gaggacggca tcttctatta
     1501 cttcgagcac agcgccgagc gacacgtgct ggtgatgagc gacaaccctg tcgccccgcc
     1561 gatcggcggc gccccggaga tctggtacag cccgccgcag ggctacgtcc gcgaccgcga
     1621 gcacatcacc cgcttcaccg tcagcgaggg catccgcacc ggcaaggtga ccctgcgtga
     1681 ccacaacctc cacaagcccg acgcgccgat ggagtcgagc gagagcgccg cgcaggcggc
     1741 cgacctcgag gtctacgact accccggcga gtaccaggac aacagctcgg gcacgacctc
     1801 caaccgcggc ccgcggctgg ccaagatccg cctcgcggcc gcgcagtcgg accgccgtcg
     1861 cgggctcggc ggcagcgact gcccgcggct gacgtcgggc cacgcgtgga cgctgacggg
     1921 ccacccgcgc ggcgacgtcg acggcgagta caagctcgtg cgcgtcgacc accgcggcga
     1981 gcagccgcag gcgctgatcc aggacgcggc cggcggcttc agctacagca acgagttcgt
     2041 gtgcatcgac gccaaggtcg cgtttcgtcc gccgcgggcg acgccgcggc cgacggtgcg
     2101 cggcgtgcag acggcgacgg tggtcggccc gcccaacgag gagatctacg tcgacgagca
     2161 cgcgcgggtg aaggtgcagt tccactggga ccgcaccgac cgcttcgacg aggagtcgtc
     2221 gtgctgggtg cgcgtcagtc agctgtgggc gggcaacacg tggggtgcga tgttcatccc
     2281 gcggatcggc cacgaggtcg tcgtcgactt catcgagggc gacccggacc ggccgatcat
     2341 catcggccgg atctaccacg gcttcaacac gccgccgtac ccgctgccgg agcagaagac
     2401 caagagcacg atcaagtcgg acacgtatca gggcgccggc tacaacgagc tgcgcttcga
     2461 ggaccagcgc gacgtcgagc aggtgttcct gcacgcccag cgcgacctcg acatccgggt
     2521 cctgcacgac cgcatggaga gcgtgctgca caaccgccac ctgacggtcg gcagcagcga
     2581 cggcggcagc aagatcggcg acttcaacga gaacatcttg cgcgaccacc acctgcagat
     2641 cgaccgcaac cgcaacgagc acatcggcgg cgacctgcgc gtgcgcgtcg gcggcatcga
     2701 cggcgtcggc cacgtcgaca ccgtgatcga gggcaaccgc cgcgagcacg tccagcagaa
     2761 ccagagcctg gagatcaccg gcaacctcaa ggtcaagatc gacggcagca gccacgagca
     2821 ggtgcagggc gacgtgcacc gcaaggtgct cggcgtcgag acggtcgagg cgctgcgcga
     2881 ggtccacctc aagtcgacca agatcgtgat cgaggcctcg cagggcctga ccatccgcgg
     2941 ccccggcggc ttcatcacga tccac
//
LOCUS       DASWNJ010001515         3225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_166415, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001515 DASWNJ010000000
VERSION     DASWNJ010001515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166415"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VGB85_25200"
     CDS             <1..394
                     /locus_tag="VGB85_25200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137415.1"
                     /translation="LPPEGTRCDNDGEECAPGKDVCAGYAGAACTQGHWKRFEVPPGT
                     PEQCQATIDCGSVCAAILAAKCPAGPADDPACASECESRVTGPCAVEFSSAVTCGDEP
                     PGFTCDASDRPTIAGCEAQFEALYGCLD"
     gene            534..1541
                     /locus_tag="VGB85_25205"
     CDS             534..1541
                     /locus_tag="VGB85_25205"
                     /inference="COORDINATES: protein motif:HMM:NF040195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010333 - terpene synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0046246 - terpene biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terpene synthase family protein"
                     /protein_id="HEY0137416.1"
                     /translation="MTAQNELRPVPATITLRYPMQWYTPVHPHAATTEAGTQAWLADL
                     RLLDSEAAVAKFRHLGVARYGGWPLYNADAEALATVTRFLTLWIFYDDMLEGQGEAHE
                     MLAVAAVRGEPDFDVEAGGPYLRAWWETACRYRTRMDTAWCERLGERFGDWLRAVREE
                     AEQLRVLREEGRHPDTLAYIHCRRRSIGVLPTLNLLEYACGCALPDELRGHPGVAALE
                     RAAAELVFIQNDLVSVPKDARSEGVNLVFSVAQERRCSLAEACHEVEKMHDTALRGFT
                     DAAEFLRIEYRDCPALLEWLAAAETMCHGFARWHLSNTRYDRELPLGDGTSVRIEVEY
                     L"
     gene            complement(1591..2358)
                     /locus_tag="VGB85_25210"
     CDS             complement(1591..2358)
                     /locus_tag="VGB85_25210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEY0137417.1"
                     /translation="MQVHARRPAILVVDDEPASRAATVQDLGDPFEVQATDHAGGFRA
                     AIEHAPDLMIVNVESGAAGWALVKQVRCRPERALVPCIFIAAAASPEDRLRGFQLGAD
                     DFVRKPLRPGELVQRVQGSLQRQARIVGTVRDHLQAQAGVAGTLEAVGLPALLALLEM
                     ERKTGVLSLLRRDPPHEANLYIRDGHLYDAQLGGRRPRSHAEAVYDLLRWHSGRFEFV
                     ARPLDVVDAIGLSTAELLQEGARRIDDSVVGPLPTGP"
     gene            2550..>3225
                     /locus_tag="VGB85_25215"
     CDS             2550..>3225
                     /locus_tag="VGB85_25215"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137418.1"
                     /translation="MAKPLEYNATLVEREDLTPALGVFRVRPDEALPGTGAWFVPGQY
                     MVLGLNNEVRVELGGVRRPMSIASAPERRDLVEFYIRYVDKPESENPFTHLLWPRKVG
                     ERMYVRVHPTGKFTVPDTVGADDRRIKVCVAAGTGLAPFLSMVRSEHIKDPTRSLSDY
                     VIIHGASYSADLGYREELEALAEKTGLRYLPTVSRPQQDPSWRGHTGRAENFFSPARL
                     AALEDIL"
BASE COUNT          465 a         1124 c         1171 g          465 t
ORIGIN      
        1 gctgcccccg gagggcaccc gctgcgacaa cgacggtgag gagtgcgccc cgggcaagga
       61 cgtgtgcgcg ggttatgccg gggccgcgtg cacgcagggc cactggaagc gcttcgaggt
      121 cccgccgggg acgcctgaac agtgtcaagc gacgatcgac tgcgggtcgg tctgcgcggc
      181 gatcctggcg gcgaagtgtc cggccggtcc ggcggacgac ccggcttgcg ccagtgagtg
      241 cgagtcgagg gtgaccggcc cctgcgcggt cgagttctcc tcggcggtca cctgtggcga
      301 cgagcccccg ggcttcacct gcgacgcctc cgaccgaccc acgatcgcgg gctgcgaggc
      361 ccaattcgag gcgctctatg gatgtttgga ctgaacccgc cggcgtgccg ccggacactt
      421 tacgatgtga ggccgtgatc ctcggggccg cggacgacgc tgtcactccg gggatcgcat
      481 acgttgctcc gtcgccccca tattctcctc cggatcgttc accatcgctc atgatgaccg
      541 cgcaaaatga gctgcggccg gtaccggcca cgatcaccct gcgctatccg atgcagtggt
      601 acacgccggt gcacccgcac gcggcgacga ccgaggccgg cacgcaggcg tggctcgccg
      661 atctgcggct gctcgacagc gaggcggcgg tcgccaagtt ccgtcacctc ggggtggctc
      721 gctacggcgg ctggccgctc tacaacgccg acgccgaggc gctggcgacg gtgacccgct
      781 tcctgacgct gtggatcttc tacgacgaca tgctcgaggg gcagggcgag gcccacgaga
      841 tgctggcggt cgccgcggtg cgcggtgagc cggacttcga cgtcgaggcc ggcggcccgt
      901 acctgcgcgc gtggtgggag acggcgtgcc gctaccggac gcgtatggac acggcctggt
      961 gtgagcgcct cggcgagcgc ttcggcgact ggctgcgggc cgtccgcgag gaggccgagc
     1021 agctccgggt cctgcgcgag gagggccgcc accccgacac gctggcgtac atccactgcc
     1081 gccggcggtc gatcggcgtg ctgccgaccc tcaacctgct cgagtacgcc tgcggctgcg
     1141 ccctgccgga cgagctgcgt ggacaccccg gcgtcgcggc gctcgagcgc gccgcggccg
     1201 agctggtgtt catccagaac gacctcgtgt cggtgcccaa ggacgcgcgc agcgagggcg
     1261 tcaacctcgt gttcagcgtc gcccaggagc gccgctgcag cctcgccgag gcctgccacg
     1321 aggtcgagaa gatgcacgac acggccctgc gcggcttcac cgacgcggcc gagttcctgc
     1381 gcatcgagta ccgcgactgc ccggcgctgc tcgagtggct cgcggccgcg gagacgatgt
     1441 gccacgggtt cgcccgctgg cacctgagca acacccgcta cgaccgcgag ctgccgctcg
     1501 gcgacggcac ctcggtgcgc atcgaggtcg agtacctgta gctcgcctgt ccgttcggac
     1561 aggtcgtgct cggcctcgac atcggggagc tcagggcccg gtcggcagcg ggccgacgac
     1621 gctgtcgtcg atccggcgcg cgccctcctg cagcagctcg gcggtcgaca ggccgatcgc
     1681 gtcgacgacg tcgagcgggc gggcgacgaa ctcgaagcgg cccgagtgcc agcgcagcag
     1741 gtcgtagacg gcctcggcgt gcgagcgcgg gcgccgtccg ccgagctgcg cgtcgtacag
     1801 gtgtccgtcg cggatgtaca ggttggcctc gtgcggcggg tcgcggcgca gcagcgagag
     1861 cacgccggtc ttgcgctcca tctcgagcag ggcgagcagg gcgggtaaac caacggcctc
     1921 gagcgtgccg gcgacgccgg cctgggcctg cagatgatcg cggaccgtgc cgacgatgcg
     1981 ggcctggcgt tgcagcgagc cttgcacccg ctggaccagc tcgccggggc gcagcggctt
     2041 gcgcacgaag tcgtcggcgc cgagctgaaa gccacgcagg cggtcctccg gcgaggcggc
     2101 ggcggcgatg aagatgcagg ggacgagcgc ccgctccggc cggcagcgga cctgcttgac
     2161 gagcgcccag ccggccgcgc ccgactcgac gttgacgatc atcaggtccg gcgcgtgctc
     2221 gatcgcggcc cggaagccac cggcgtggtc ggtggcctgc acctcgaacg ggtcgccgag
     2281 gtcctgcacg gtcgccgcgc gcgaggcggg ctcatcatcg acgacgagga tcgcgggacg
     2341 gcgggcgtgg acctgcatgg gcactctgtt gggttggacc ggctcaatgc cgattgccat
     2401 caagtggccg cgaataattt caagtgatcc ggcacaccgt acctcgcggc gatacgcgca
     2461 gaaaaacggt gttatttccg gctccaggta tacccggtgt ctgcgtcggc gctggcgagg
     2521 ctcgcgccgc tcgggcatga tgcggtccca tggccaaacc gctggagtac aacgcgacgc
     2581 tggtcgagcg tgaagatctg acccccgcgc tcggggtgtt tcgggtccgc cccgacgagg
     2641 ccctgccggg gaccggcgcg tggttcgtgc ccgggcagta catggtgctc ggcctcaaca
     2701 acgaggtccg cgtcgagctc ggcggggtgc gccggccgat gtcgatcgcg tcggcgccgg
     2761 agcggcgcga cctcgtcgag ttctacatcc gctacgtcga caagccggag tcggagaacc
     2821 cgttcacgca cctgctgtgg ccgcgcaagg tcggcgagcg catgtacgtg cgcgtgcacc
     2881 cgaccggcaa gttcacggtg cccgacacgg tcggcgccga cgaccgccgg atcaaggtct
     2941 gcgtggccgc cggcaccggc ctcgcgccat ttttgtcgat ggtccgcagc gagcacatca
     3001 aggatccgac gcgctcgctg tccgactacg tgatcatcca cggcgcgagc tacagcgccg
     3061 acctcggcta ccgcgaggag ctcgaggcgc tggcggagaa gaccggcctg cgctacctgc
     3121 cgacggtcag ccgcccgcag caggacccga gctggcgcgg acacactggc cgcgccgaga
     3181 acttcttctc gcccgcgcgc ctggccgcgc tcgaggacat cctcg
//
LOCUS       DASWNJ010001516         2141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_27448, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001516 DASWNJ010000000
VERSION     DASWNJ010001516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27448"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VGB85_25220"
     CDS             <1..478
                     /locus_tag="VGB85_25220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137419.1"
                     /translation="GMAWTILRPVFFMQSLLFFADPIKTGKIIAATGAGEVAFVDARD
                     VAEVAATVLAAPGEDAIHTLTGARALSFAALAGLLSTALGRPVRHISPPRWLARAALP
                     WLSGMPRWQSNMVVDLMAAIARGAQAVPTDDVPRILGREARTIENFIAEQRAAFGG"
     gene            complement(482..1645)
                     /locus_tag="VGB85_25225"
     CDS             complement(482..1645)
                     /locus_tag="VGB85_25225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0137420.1"
                     /translation="MSEHDEIVRAIFDNCEFFTFAIGRDGVCTFIEGRAAAELGDLGA
                     FRNANIYEMLGPDSPIAVAVRRVLAGETFTTQFELAGRVLQVRYAPLRDAAAVDRIKG
                     AVGIAIDITELVLAQQDSVRTAQRLREKNDLLDLARDAILMRRPDGTLTYWNRGAEQL
                     YGYTADEAVGAVSHVLLQTVFPVDAREIDRQLLAEGHWEGDLMHRRRDGSQVYVSSRW
                     MLRRDEHGAVLEMFEINTDITARRAAEEAQKQRQEEIIRAQAHAIAELSTPMIPITDG
                     VLVMPLIGMMDSMRAKQVMDALLSGLAQNRGRVAILDITGVPVVDTATANALIQAAHA
                     ARLLGAEVILTGIRPEVAQTLVTLDAKLGSITTRGTLQSAIELVLGRRRLGGA"
BASE COUNT          282 a          730 c          801 g          328 t
ORIGIN      
        1 gggcatggcc tggaccatcc tgcgcccggt gttcttcatg cagtcgctgc tgttcttcgc
       61 cgacccgatc aagaccggca agatcatcgc ggcgaccggc gcgggcgagg tcgcgttcgt
      121 cgacgcgcgc gacgtcgccg aggtcgccgc gaccgtgctc gcggcgccgg gcgaggacgc
      181 gatccacacg ctgaccggcg cgcgcgcgct gagcttcgcg gcgctcgccg ggctgctgtc
      241 gacggcgctc ggacgtcccg tgcgccacat cagcccgccg cgctggctcg cccgcgcggc
      301 cctcccgtgg ctcagcggca tgccgcgctg gcagtcgaac atggtggtcg acctgatggc
      361 ggcgatcgcc cgcggcgcgc aggcggtgcc caccgacgac gtgccgcgga tcctcggacg
      421 cgaggcgcgg acgatcgaga atttcatcgc ggagcagcgc gcggcgttcg gtggatgacg
      481 ctcacgcgcc gccgaggcga cggcggccga gcacgagctc gatcgcgctc tgcagggtgc
      541 cacgcgtggt gatcgatccg agcttggcgt cgagcgtcac gagggtctgc gcgacctcgg
      601 ggcggatgcc ggtcaggatc acctcggcgc cgagcaggcg ggcggcgtgg gcggcctgga
      661 tcagcgcgtt ggcggtcgcg gtgtcgacga ccggcacgcc ggtgatgtcg aggatcgcca
      721 cgcgcccgcg gttctgggcc aggccgctga gcagggcgtc catcacctgc ttggcccgca
      781 tcgagtccat catgccgatc agcggcatca cgagcacgcc gtcggtgatc gggatcatcg
      841 gcgtcgagag ctcggcgatc gcgtgggcct gggcgcggat gatctcctcc tggcgctgct
      901 tctgcgcctc ctcggcggcc ctgcgcgcgg tgatatcggt gttgatctcg aacatctcga
      961 gcaccgcgcc gtgctcgtcg cggcggagca tccaccggct cgacacgtac acctggctgc
     1021 cgtcgcggcg tcggtgcatc aggtcgccct cccagtggcc ctcggcgagc agctgacggt
     1081 cgatctcgcg ggcgtcgacc ggaaagacgg tctgcagcag gacgtgtgat acggcgccga
     1141 ccgcctcgtc cgcggtatag ccgtacagct gctcggcgcc gcggttccag taggtcagcg
     1201 tgccgtccgg gcggcgcatg aggatcgcgt cgcgcgcgag gtcgaggagg tcgttcttct
     1261 cgcgcagccg ctgggccgtg cgcacgctgt cctgctgggc gagcacgagc tcggtgatgt
     1321 cgatggcgat cccgacggcg cccttgatcc tgtcgaccgc cgccgcgtcg cgcagcggcg
     1381 cgtagcggac ctgcaggacc cgccccgcga gctcgaactg ggtggtaaag gtctccccgg
     1441 cgagcacccg tcgcaccgcg acggcgatcg gcgagtccgg gccgagcatc tcgtagatgt
     1501 tggcgttgcg aaacgcgccg aggtccccga gctcggccgc ggcccggccc tcgatgaagg
     1561 tgcagacgcc gtccctgccg atcgcgaagg tgaaaaattc gcagttgtcg aagatggccc
     1621 ggacgatctc gtcgtgctcg ctcatggtga atccctcggg tcggtccact cgacgtgtct
     1681 atcatcgaga tagaaccgcc gcgcccgcaa gtttggcggc gcggcggtcg cggtgacgct
     1741 gcacgacggc gggtcgcata cgcagacacg ccgcgatcat cgagcggcag cgcgcgtcgt
     1801 gacgtcagca acaggacgat cgacggcgta gcggcctgcc cggcgcacga cgcagacgcc
     1861 gtcggacgtc ggttgatgtg gctggtggtc gtacggcgag tcggatcggt cggcgcgcat
     1921 cgggtatgag cgcgagcgcg cgacctgcac gtggtcggca gcgtgttcgt ggcgctggtg
     1981 ctgctcggcg gcggagcgtg ggcctgacag cgggcccacg agatccagcc cgtcgccgcc
     2041 tgcggccggt tcggggcggc gctccttcgg gcggatcgcc ggcgcacccg aaggcggccc
     2101 gagggcacga gagcctggac gtcgtcggtg gagccggcgt t
//
LOCUS       DASWNJ010001517         2142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_448943, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001517 DASWNJ010000000
VERSION     DASWNJ010001517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448943"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="VGB85_25230"
     CDS             <1..1096
                     /locus_tag="VGB85_25230"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0137421.1"
                     /translation="ITGPSVVTITGEPWKRRRRLLDPAFRKEPLARHARIVRARAREH
                     ADRWLHAGAIEAHGALLEFMFDTLIDALTGAELGPDRAVLRRGFEVFWADFSSREFAL
                     LCALTNGEPYARLTTPRRWRQKRLLGEFDGILARLARHAREAPGEDMLSALLPSVDRE
                     GGLDEQELREDVITVILAAQETTAMGLTMTLDLLARHPEVAARVAAEAAALPPDAATL
                     DDLKLLPYTDAVVREALRLYPPIWGVAREATAPLTLRGHAIAPGALLVASAWVVQHDE
                     RWWAPDPLAFRPERWLSECERPRLAWFPFGAGPHLCIGMRLALMEMVFAVAEWSQRLH
                     FHASTPPPELITTLTMRPKHGVPLRVTARD"
     gene            complement(1127..1714)
                     /locus_tag="VGB85_25235"
     CDS             complement(1127..1714)
                     /locus_tag="VGB85_25235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137422.1"
                     /translation="MKLRTSLLLTLTALTLGLASTDAAASNYPPDYDVCSATETRYSG
                     PFEVIRDFVDLYDDHMKLTVAYDGYLRDLYADDQINIYIVLNGNDAFIEALPGTHDDA
                     YIFLDSGPRGCHWCGNGWNPPGTCDGVVFNQYESGKWICGTPTAVEDHLFEWAFNGYG
                     QLNAWDIELAAEAGGQWDSNFGANFDVRFEPRGCY"
     gene            1848..>2142
                     /locus_tag="VGB85_25240"
     CDS             1848..>2142
                     /locus_tag="VGB85_25240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137423.1"
                     /translation="MRRSDVRGSPAPRARPPAVAVAAIAMMIAGGAALLGMQSCAQTP
                     DAQAPGGTLGQHPLQQIDPPRQPVVQPDPPPQPRPEPQRVVQPDPPPQPRPEPQ"
BASE COUNT          294 a          831 c          715 g          302 t
ORIGIN      
        1 catcaccggg ccgagcgtcg tgacgatcac gggtgagccg tggaagcggc gccgccggct
       61 gctcgacccc gcgtttcgca aggagccgct ggcccgccac gcccgcatcg tccgcgcccg
      121 cgcccgcgag cacgccgacc gctggctgca cgccggcgcg atcgaggccc acggcgccct
      181 gctcgagttc atgttcgaca ctttgatcga tgcgttgacc ggcgccgagc tcgggcccga
      241 ccgcgccgtg ctgcgccgcg gcttcgaggt gttctgggcc gacttctcct cgcgcgagtt
      301 cgccttgctg tgcgcgctca ccaacggtga gccctacgcg cgcctgacca cgccgcgccg
      361 ctggcgacag aagcggctgc tcggcgagtt cgacggcatc ctcgcccgcc tcgcccgcca
      421 cgcgcgcgag gcccccggcg aggacatgct ctcggccctg ctgccctccg tcgaccgcga
      481 gggcggcctc gacgagcagg agctgcgcga ggatgtcatc accgtcatcc tcgccgcgca
      541 ggagacgacc gcgatgggcc tgacgatgac cctcgacctg ctcgcgcgcc accccgaggt
      601 cgccgcgcgc gtcgccgccg aggccgccgc gctgcccccg gacgccgcca cgctcgacga
      661 cctcaagctg ctgccctaca ccgacgcggt cgtgcgcgag gccctgcgcc tgtatccgcc
      721 gatctggggc gtcgcccgcg aggcgaccgc cccgctgacc ctgcgcggcc acgcgatcgc
      781 gccgggcgcg ttgctggtcg cgtccgcctg ggtggtacaa cacgacgagc gctggtgggc
      841 ccccgacccg ctcgcgtttc gccccgagcg ctggctcagc gaatgcgagc gcccgcgcct
      901 cgcctggttc cccttcggcg ccggccccca cctgtgcatc ggcatgcgcc tcgccctgat
      961 ggagatggtg ttcgccgtcg ccgaatggtc acagcgcctg cactttcatg cgagcacgcc
     1021 gccgcccgag ctgatcacca cattgacgat gcggcccaag cacggcgtgc ccctgcgcgt
     1081 cacggcgcgc gactgagtcc gtgcccctgg ccagtcgcgc gaacgatcaa tagcaaccac
     1141 gcggctcgaa gcggacgtcg aagttcgccc cgaagttgct gtcccactgc cccccggcct
     1201 cggcggcgag ctcgatgtcc catgcgttga gctggccgta cccattaaaa gcccactcga
     1261 acaggtggtc ctcgaccgcg gtcggggtcc cgcagatcca cttgccgctc tcgtattgat
     1321 tgaacacgac cccgtcgcac gtgcccggcg ggttccaccc gttgccgcac cagtgacagc
     1381 cgcgcgggcc ggagtcgagg aagatgtacg cgtcgtcatg ggtgcccggc agcgcctcga
     1441 tgaacgcgtc gttgccgttg agcacgatgt agatgttgat ctgatcgtcg gcatagaggt
     1501 cgcgcagata tccgtcgtag gcgaccgtca gcttcatgtg gtcatcgtac agatcgacga
     1561 aatcgcggat cacctcgaag ggcccggagt accgcgtctc ggtcgcgctg cagacatcgt
     1621 agtccggcgg atagttcgag gcggcggcgt cggtcgacgc gaggccgagc gtgagggcgg
     1681 tcagcgtgag caggagcgag gtgcgaagct tcatggcgcg cgcactgtag atcgccggcc
     1741 cggccgcggg accggtgacg cgcgtgatcg gccgaggcgc gcacgagcgg tcgcgcactc
     1801 gcggcctcgc cgatcgccgc caggcccgac cgtgataccg tccgacgatg cgtagatccg
     1861 acgtgcgtgg atccccggcg ccgcgcgccc gaccgccggc cgtcgcggtc gctgcgatcg
     1921 ccatgatgat cgcaggcggc gccgcgctgc tcggcatgca aagctgcgcc cagacccccg
     1981 acgcgcaggc tcccgggggc acgctcggcc agcacccgct gcagcagatc gacccgccgc
     2041 gccagccggt cgtgcagccc gacccgcccc cgcagccccg ccccgagccc cagcgggtcg
     2101 tgcagcccga cccgcccccg cagccccgcc ccgagcccca gc
//
LOCUS       DASWNJ010001518         1534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_63186, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001518 DASWNJ010000000
VERSION     DASWNJ010001518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63186"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VGB85_25245"
     CDS             <1..278
                     /locus_tag="VGB85_25245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137424.1"
                     /translation="HEQNDPLYGRLATIHNIFFFHQWVGALRTSLHQGNFREVAAKFS
                     SIITDFYLTKPQPPPGKARAPRPAVAPPPAGDVAPDATAADDSGAAS"
     gene            389..757
                     /gene="yajC"
                     /locus_tag="VGB85_25250"
     CDS             389..757
                     /gene="yajC"
                     /locus_tag="VGB85_25250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEY0137425.1"
                     /translation="MLATPSLFLLAEATAAGSPLGGFLPIILMVGVFYFLVIRPQGKL
                     EKDRKARLAKLDKGDQVRLNGGILGRVTSVEDQIVMVEIADKVRVKVLRAEIADKWEP
                     PAVSGTVVKTDTAVATEVRS"
     gene            764..>1534
                     /locus_tag="VGB85_25255"
     CDS             764..>1534
                     /locus_tag="VGB85_25255"
                     /inference="COORDINATES: protein motif:HMM:NF045451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137426.1"
                     /translation="MRRFLKTKALLLLALITLSVLMVVPSIYQILGRDKELPGWITNT
                     FKNRFKLGLDLQGGLHLEYSVAVDEALENKLGQIASELETAFKEKKGVAVTASPIGLD
                     RIDITFIDPAQVATAEDDVLGIALSDMERVDTADEASGVIHLKMLDERLAEHRSSAVG
                     QALDTVRRRIDAMGIAEPNIYPKNRQIVIELPGLSDTTTEIKAARKDIIVKLRPILLA
                     AGAGQITVSESAREPEAFKLTMPGTTARDVLAAAFKDGL"
BASE COUNT          268 a          577 c          459 g          230 t
ORIGIN      
        1 tccacgagca gaacgacccg ctgtacggcc ggctcgccac gatccacaac atcttcttct
       61 tccaccagtg ggtcggcgcc ctccgaactt ccttgcacca gggcaacttc cgcgaggtgg
      121 cagccaagtt ttcttcaatt atcacggatt tttacctcac taaacctcaa ccaccaccgg
      181 gcaaagctcg ggcgcccaga cccgccgtcg cgccgccgcc ggccggggat gtcgcgcccg
      241 acgccaccgc cgcggatgac agcggcgccg cttcctgata ggcccctgct cccggcgggg
      301 gcctgcgttt ttgcacgcgc ccgcgggcgc ccggggccgt cagaagccgc ggtcgccggt
      361 ggatactgcc cccgactcga gcctcgccat gcttgcaaca ccctccctgt tcctcctcgc
      421 cgaagccacc gccgccggta gcccgctcgg cggcttcttg ccgatcatcc tgatggtcgg
      481 ggtgttctat ttcctcgtca ttcgccccca gggcaagctc gagaaggacc gcaaggcgcg
      541 tctggccaag ctcgacaagg gtgaccaggt ccgcctcaac ggcggcatcc tcggccgcgt
      601 gaccagcgtc gaggaccaga tcgtcatggt cgagatcgcc gacaaggtcc gcgtcaaggt
      661 gctgcgcgcc gagatcgccg acaagtggga gccgccggcc gtcagcggca ccgtcgtcaa
      721 gaccgacacc gcggtcgcca cggaagtccg gagctaagag ccaatgcgtc gtttcctcaa
      781 gaccaaggcc ctgctgctgc tcgcgctgat caccctcagc gtgttgatgg tcgtgccgtc
      841 gatctaccag atcctcggcc gcgacaagga gctgccgggc tggatcacca acaccttcaa
      901 gaaccgcttc aagctcggcc tcgacctcca gggcggcctg cacctcgagt actcggtcgc
      961 cgtcgacgag gcgctcgaga acaagctcgg ccagatcgcc agcgagctcg agaccgcctt
     1021 caaggagaag aagggcgtcg ccgtcacggc ctcgccgatc ggcctcgacc gcatcgacat
     1081 caccttcatc gacccggccc aggtcgccac cgccgaggat gacgtcctcg gcatcgcgct
     1141 gtccgacatg gagcgcgtcg acacggccga cgaggcgagc ggcgtcatcc acctcaagat
     1201 gctcgacgag cggctcgccg agcaccgcag ctccgcggtc ggccaggcgc tcgacaccgt
     1261 ccgccggcgc atcgacgcca tgggcatcgc cgagcccaac atctacccga agaaccgcca
     1321 gatcgtgatc gagctgccgg gtctgagcga cacgacgacc gagatcaagg ccgcgcgcaa
     1381 ggacatcatt gtcaagctgc gcccgatcct cctcgccgcc ggcgccggtc agatcaccgt
     1441 ctcggagagc gcccgcgagc ccgaggcctt caagctgacg atgccgggca cgaccgcccg
     1501 cgacgtcctg gcggccgcct tcaaggacgg cctc
//
LOCUS       DASWNJ010001519         2987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_275314, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001519 DASWNJ010000000
VERSION     DASWNJ010001519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275314"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VGB85_25260"
     CDS             complement(<1..199)
                     /locus_tag="VGB85_25260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137427.1"
                     /translation="MSPSSPPRSTKFARVKQRLFKDASSFDTSGALFVPLPIVLRIVL
                     HRFSPREWQVLSWIYMSCGKEG"
     gene            682..1065
                     /locus_tag="VGB85_25265"
     CDS             682..1065
                     /locus_tag="VGB85_25265"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY0137428.1"
                     /translation="MNKPTSLLPAAALSLATLTGAALAPSTAEADTHQHSAQVREIEV
                     IVDGGYQPGRITVTAGEHVRLRFIRRDHTPCTREVVFPGLDIRRELPTDQPVVIDLPA
                     LAVGEYEFRCGMNMIRGLLVVQPKP"
     gene            1153..1737
                     /locus_tag="VGB85_25270"
     CDS             1153..1737
                     /locus_tag="VGB85_25270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY0137429.1"
                     /translation="MTTADQDPQFLPDTPAAVARLVANHREFLGFLENRVGSRALAED
                     ILQDAFIRGIGKLQSLREDESAVAWFYRVLRNAVIDHHRRHEVADRRLAALAAELDTS
                     EPDDEVRGAVCQCVARLADNLKPEYAEALRRIEVDGVAVKDYAAEAGITSNNAAVRVF
                     RAREALKKQVVRSCGTCADHGCLDCTCSAGHCGE"
     gene            complement(1955..2536)
                     /locus_tag="VGB85_25275"
     CDS             complement(1955..2536)
                     /locus_tag="VGB85_25275"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEY0137430.1"
                     /translation="MLAASVAAGAPAVPMTRRRMRSIKKFSILVLGVSAIIACDSQHH
                     DAGATSPRSDVEFIDAMIPHHEMALEMTDMVLQKGSDVEVKAMAQAMKDAQATEIAEM
                     KVIRTRLAGSAETTAHHDEHMMADMEKLAATSGAALDRLFLEEMLPHHAGAIKMAHEA
                     LPYLEETELQVMANKIVYDQAREIGDIAAMLEE"
     gene            complement(2648..>2987)
                     /locus_tag="VGB85_25280"
     CDS             complement(2648..>2987)
                     /locus_tag="VGB85_25280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137431.1"
                     /translation="NCMTTTDEKIDELARQVEAMTVALERLYALLSERPATGGKRRAK
                     PSPAQLAELQKTTAYKARVARAAKRYGVPLSEWLERFGPVDKLPPGVPRPAPTLPVTP
                     YRRTVTARSS"
BASE COUNT          491 a         1066 c          918 g          512 t
ORIGIN      
        1 caccctcctt cccgcagctc atgtagatcc acgacaagac ctgccactca cgtggcgaga
       61 acctgtgcag cacgatgcgc aagacgatcg gcaagggaac gaagagagcg ccgctcgtat
      121 cgaaggacga agcgtccttg aacaggcgct gcttgaccct ggcgaacttt gtgctcctcg
      181 ggggtgacga cggggacatg ggcaccgatc tactgcctca cgccacttct tccaaaccac
      241 cccttacccc ctccgccaac caccccggtc aatcccacaa caccatcaat caacgtcaac
      301 acagtcaatg cagttcctgg atacaaaaaa ctggaaaaat gtttatttcc aacaatctat
      361 gacttggcat gtaattttcc gggacggccc aaggcccttt gaaaactccg tttgggccgt
      421 cgacccccct ctacaaaagt ctacaatact tggcttggcc tccatgcgag ctacctaaac
      481 gggctctgag cgaacgccgc gttgcgaccg ccgcagccgt cgtcctcacc ccgcagcggc
      541 tccgccacct gaatcaccgg ccccatcgcg ccctcgcacc gcgggctggt tccttcgtcc
      601 cgcagacacg ccgacggcga aggtggcggg caggtgtaat gccccgccgg tcgctgcgtc
      661 agcgcttgca ggaccgacac catgaacaag ccaacctccc ttctccctgc cgccgccctc
      721 tcgctcgcca ccctgaccgg cgcggccctt gccccctcga ccgccgaggc cgacacccac
      781 cagcacagcg cgcaagtgcg tgagatcgag gtcatcgtcg acggcggcta ccagcccggc
      841 cgcatcaccg tgaccgcggg cgagcacgtc cgcctccgct tcatccgccg cgaccacacg
      901 ccctgcaccc gcgaggtcgt gttccccggc ctcgacatcc gccgggagct gccgaccgat
      961 cagccggtgg tcatcgacct ccccgcgctc gccgtgggtg agtacgagtt ccgctgcggg
     1021 atgaacatga tccgcgggct cctggtcgtg cagcccaagc cctgagcaca acctcgcgcc
     1081 tgccggccgc ggccggcagg cgcgctcgca gcgacgctat actcgactct gagacccgcc
     1141 cgaggtgccg ccatgacgac cgccgaccag gacccgcaat ttctccccga cacccccgcg
     1201 gccgtggcac gtctcgtcgc caaccaccgc gagtttctcg gcttcctgga gaatcgcgtc
     1261 ggcagtcggg cgctggccga ggacatcttg caagacgcgt tcatccgcgg gatcgggaag
     1321 ctccagagcc tgcgcgagga cgagtcggcg gtcgcctggt tctaccgggt cctccgcaac
     1381 gccgtgatcg accatcaccg tcgccacgag gtcgcggacc ggcggctcgc agccctcgcg
     1441 gccgagctcg acacgtccga gcccgacgac gaggtccgcg gcgccgtgtg tcagtgcgtc
     1501 gcccgcctcg ccgacaacct caagcccgag tacgcggagg ccctgcggcg catcgaggtc
     1561 gacggcgtcg cggtcaagga ctacgcggcg gaggcgggca tcaccagcaa caacgccgcg
     1621 gtgcgggtgt tccgtgcgcg cgaggcgctg aagaagcagg tcgtgcgctc ctgcgggacc
     1681 tgtgccgatc atggctgcct cgactgcacc tgcagcgccg gccactgcgg cgaataacct
     1741 ggtacggggc ctgctaggca cccgcgagcc acttcctcat gcgttttttc tggactcccg
     1801 tctgtccctg ccctggtgcg ccgtgctcgc agcgagccgg cggtgcgccg agatggtcag
     1861 acccggcggt gacgacggtt caggccgtgc cccgcgacgc ggggccctgc actcggtcgg
     1921 gttcatcgtg gcctgggcgc ctgtcactgc gtgatcactc ctcgagcatc gcggcgatgt
     1981 cgccgatctc cctggcctgg tcatagacga tcttgttggc catcacctgc agctcggtct
     2041 cttcgagata cggcagcgcc tcgtgagcca tcttgatggc gcccgcgtga tgcggcagca
     2101 tctcctcgag gaacaggcga tcgagggcgg cacctgacgt ggccgcgagc ttctccatgt
     2161 cggccatcat gtgctcgtca tgatgtgctg tcgtctcggc cgaacctgcg agccgcgtcc
     2221 gaatcacctt catctcggcg atctcggtgg cctgagcatc cttcatcgcc tgggccatcg
     2281 ccttcacctc cacgtcgctg cccttttgca gcaccatgtc ggtcatctcg agggccatct
     2341 cgtgatgcgg gatcatggcg tcgatgaatt cgacgtcgga cctcgggctc gtcgcgccgg
     2401 catcgtgatg ctgcgagtcg cacgcaatga tcgccgagac gcccagtacc aggatggaga
     2461 atttcttgat ggagcgcatg cgtctccgtg tcatgggtac ggccggtgcg ccagcagcga
     2521 cggaggccgc gagcacagcg caccacgcga agcggacgcc tgcaaccgcg gcgctgttac
     2581 acatgtctca ggccgcttga ggatattctt aagcagccga agtagccgtg gccagcgccc
     2641 aaggcaccta cgagcttcgc gcggtaactg tccgtcggta gggcgtcacg ggcaaggtcg
     2701 gggcgggccg tggcacgcca ggcgggagct tgtcgacggg cccgaagcgc tcgagccact
     2761 cggagagggg cactccgtag cgcttcgcgg cccgggccac gcgggccttg tacgccgtgg
     2821 tcttctgcag ctcggcgagc tgggcgggcg acggcttcgc ccggcgcttg ccgccggttg
     2881 cgggtcgctc gctgagcagg gcgtaaaggc gctccagggc aacggtcatc gcctcgacct
     2941 gtcgggcgag ctcgtcgatt ttttcgtcag tcgtcgtcat acaattt
//
LOCUS       DASWNJ010001520         1699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_27567, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001520 DASWNJ010000000
VERSION     DASWNJ010001520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27567"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VGB85_25285"
     CDS             <1..438
                     /locus_tag="VGB85_25285"
                     /inference="COORDINATES: protein motif:HMM:NF021631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY0137432.1"
                     /translation="PELLQLQALARGAVSRRCARHYQGFFRGMCREHERAEKPRAKTL
                     LYAYRVALTGIHLLRTGELVADLRTLAPQLGYPDALSLIHLKVLGAEKGAVTPEVDSQ
                     HRAAWPALEADLQRALEHSPLPEEAANEGACEAWLVDLRCARL"
     gene            512..1564
                     /locus_tag="VGB85_25290"
     CDS             512..1564
                     /locus_tag="VGB85_25290"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137433.1"
                     /translation="MAHIVIDTCAGRECGAEALEAAAIASLEHEHQITVVGDESEITA
                     ALQRLAHDAEHLAVAHAGDRIDPELELGEALDRAPRSSIAVGMGLIARDPGAIFLSAG
                     GTAAIVATASRVLGRLPGVRNPALAAVYPTLRPVGPASDPFALLLDVGATVHCDADDL
                     VNFAAMGAAYAAKISRIERPRVALLSNGTGLAGAPERVRGAHRRLSQATPGFEYVGYL
                     RADHVTSGDADVIVTDGFTGDVLLRTLEGVAATGELLIKRAAERLQWRLGVQMLGDGL
                     AAIREFTDWENYGGGALLGLDRAVVVTQANSGRRAYLNAIRLGAKIDRLQVLAAVAAS
                     VAALRDPPPTPTEAAT"
BASE COUNT          225 a          701 c          562 g          211 t
ORIGIN      
        1 ccggagctgt tgcagttgca agcgctcgcc cgcggcgcgg tctcgcggcg gtgcgcgcgg
       61 cactaccagg gttttttccg cggcatgtgc cgggagcacg agcgcgccga gaagccgcgc
      121 gccaagaccc tgctgtacgc ctaccgcgtc gcgctgaccg gcatccacct gctgcgcacc
      181 ggcgagctcg tcgccgacct gcgcaccctg gccccgcagc tcggctaccc ggacgccctg
      241 agcctgatcc acctcaaggt cctcggcgcc gagaaggggg cggtcacgcc cgaggtggat
      301 tcgcagcacc gggcggcctg gcccgcgctc gaggccgacc tgcagcgcgc cctcgaacac
      361 agccccttgc cggaggaggc cgccaacgag ggcgcctgcg aggcctggct ggtcgacctg
      421 cgctgcgcac ggctgtgacc acctcgggga tcagcctggc acgcggacgc cgctggccat
      481 gcggcgtccg ggtttggtag aaggcgagac cgtggcgcac atcgtcatcg acacctgtgc
      541 cggacgcgag tgcggcgccg aggccctcga ggccgccgcg atcgcgtccc tcgagcacga
      601 gcaccagatc accgtcgtcg gcgacgagtc cgagatcacc gccgcgctgc aacgcctcgc
      661 gcacgacgcc gagcacctgg ccgtcgccca cgccggcgac cgcatcgacc ccgagctcga
      721 gctcggcgag gcgctcgacc gggccccgcg atcgagcatc gccgtcggca tgggcctgat
      781 cgcccgcgac cccggcgcga tcttcctctc ggccggcggc accgcggcga tcgtcgccac
      841 cgcgtcgcgt gtgctcggca ggctgcccgg cgtgcgcaac ccggcgctcg cggccgtgta
      901 cccgaccctg cgccccgtcg gccccgcctc cgaccccttc gcgctgctgc tcgacgtcgg
      961 cgccaccgtg cactgcgacg ccgacgacct ggtcaacttc gcggcgatgg gcgccgccta
     1021 tgccgccaag atctcccgca tcgagcgccc gcgcgtcgcc ctcctgtcca atgggacagg
     1081 tctcgccggc gcccccgagc gggtccgcgg cgcgcaccgg cggctctcgc aggcgacgcc
     1141 tggcttcgag tacgtcggct acctgcgcgc cgaccacgtc acctccggcg acgccgacgt
     1201 catcgtcacc gacggcttca ccggcgacgt cctgctgcgc acgctcgagg gcgtcgccgc
     1261 gaccggcgag ctgctgatca agcgggccgc cgagcgcctg cagtggcgcc tcggcgtgca
     1321 gatgctcggg gacggcctcg ccgcgatccg cgagttcacc gactgggaga actacggcgg
     1381 cggcgccctg ctcggcctcg accgcgccgt cgtcgtcacg caggccaact cgggccgccg
     1441 cgcctatctc aacgcgatcc gcctcggcgc caagatcgat cgcctccagg tgctcgcggc
     1501 cgtcgccgcc agcgtcgccg ccctgcgcga cccgcccccc actcccaccg aggccgcgac
     1561 atgacgtccc acgagccacc cgacacgacc cccgagagca cgacgatcgt gacgaccgcc
     1621 gtcggtacca ctgtcggcac caccaccgtc gagccgaccg ccgacgtgac cgtcgaggcc
     1681 ccgccgaacc caccgtccg
//
LOCUS       DASWNJ010001521         2658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_486506, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001521 DASWNJ010000000
VERSION     DASWNJ010001521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486506"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..657)
                     /locus_tag="VGB85_25295"
     CDS             complement(178..657)
                     /locus_tag="VGB85_25295"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY0137434.1"
                     /translation="MDFRRFLPWRKHKTDESETTPATRVESPAPSFWRDLGGFSSLTS
                     FAGGAFAPTIDMAQTEDAYIIRAHLRGMTDRDVTITTYQGVLTIKGEQRHEREDRSRG
                     LYFVERSHGVFYRSLTLPVDAVEDGAEATFKDGVLTIEIPRSQEVRPGARMITVKTG"
     gene            785..1738
                     /locus_tag="VGB85_25300"
     CDS             785..1738
                     /locus_tag="VGB85_25300"
                     /inference="COORDINATES: protein motif:HMM:NF019610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin"
                     /protein_id="HEY0137435.1"
                     /translation="MPVLRRSLLAALATLPVVAAVSTAHARFKPPGKAARRAAIGPLD
                     DLSPELQRAFALTDDVAPMPAPGRQDWLASHAEPGQTFAQFKRARPNRPDLVRNKIVL
                     QPIGALGDGSPAQRTLVDFAKRYFGMSAIARPAVTVQSLRARSRVRGHRQYRTGDILA
                     ALRRDIPADAYCLIGVTQVDLYPGPAWNFVFGEALLYERVGVYSFARYDPKFYDEPRN
                     AGTPVQILRRGFKLMAHEIGHMFAIDHCTHSHCVMNGTNNLEETDRSPLHPCPMCLRK
                     LHEIVGFDVAARERELGEFYRALGLTSEADDCDRRLARIVG"
     gene            complement(1763..2248)
                     /locus_tag="VGB85_25305"
     CDS             complement(1763..2248)
                     /locus_tag="VGB85_25305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137436.1"
                     /translation="MSIFARHNSILRLSSFCVLVMLAACGGKDGPGTETTGDTDATSS
                     GTTVTGTGTTMPGTDTTTTGAGTTTQDTGTTTQDTDVVTGSGGENPCVASCASEEECT
                     VDEDASTGVGGDSCDDNSHCVVRMDCACILSFCELDCDPADPATCEEGKTCDAGTGLC
                     V"
     gene            2373..>2658
                     /locus_tag="VGB85_25310"
     CDS             2373..>2658
                     /locus_tag="VGB85_25310"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY0137437.1"
                     /translation="MARNAATTDVFAAVAEPSRRRILELLAPGERPVNDVARLLHLTQ
                     PTASKHLKVLKDVGLVHVREAGQQRFYSVNGRALEPIHAWSRQFEAYWNDS"
BASE COUNT          405 a          971 c          861 g          421 t
ORIGIN      
        1 cgttgaaccg gtcctcgagg atgctctgcg tgaccggtcc gccgtcgagg agcatcgacg
       61 catgctgctc gaacgcgttg ggggtctggc agcgcgggca cgggtgggtg aaggtctggc
      121 tgagcggcat gaggcggcgg atgatgcacg acgcccggcc gccgccgcga ccccgatcta
      181 tccggtcttg accgtgatca tccgcgcgcc aggacggacc tcctgcgagc gggggatctc
      241 gatcgtcagc accccgtcct tgaacgtcgc ctcggcgccg tcctccacgg cgtcgaccgg
      301 cagcgtgagg ctgcgataga acacgccgtg gctgcgctcg acgaagtaca gcccgcgcga
      361 gcgatcctcg cgctcgtgtc gctgctcgcc cttgatcgtc agcacgccct gatatgtcgt
      421 gatcgtgacg tcccggtcgg tcatgccgcg caggtgcgca cgaatgatat acgcgtcctc
      481 ggtctgcgcc atgtcgatcg tcggggcgaa cgcgccgcca gcgaaggagg tcagcgagga
      541 gaatccgccc agatcccgcc agaagctcgg ggctggcgac tcgacgcgcg tcgccggcgt
      601 ggtctccgat tcgtcggtct tgtgctttcg ccagggaaga aaacgccgaa aatccatggt
      661 cgtcctcctt gtctcgagga cactgtttaa tcgcgtccgc gcgtccgtca accgccgcga
      721 tgtgcgcagt atcgacccca cgccgcgccc gggaaccgat gaccccgctc gcgcgttgtc
      781 ccgcatgccc gtgcttcgcc gcagcctcct cgccgcgctc gcgaccctgc ccgtcgtcgc
      841 cgcggtctcg accgcccacg cgaggttcaa gccgcccggc aaggccgccc ggcgcgccgc
      901 gatcggcccg ctcgacgacc tctcgccgga gctgcaacgg gccttcgcgc tgaccgacga
      961 cgtcgcgccg atgccggcgc ccggccgcca ggactggctc gcctcgcacg ccgagcccgg
     1021 ccagaccttc gcccagttca agcgcgcgcg ccccaaccgc cccgacctcg tacgcaacaa
     1081 gatcgtcctc cagccgatcg gtgcgctcgg cgacggcagc ccggcgcagc gcaccctggt
     1141 cgacttcgca aagcgctact tcggcatgtc ggcgatcgcc aggccggcgg tgacggtgca
     1201 gagcctgcgc gcccgctcgc gggtccgcgg ccaccggcag tatcgcaccg gcgacatcct
     1261 cgcggccctc cggcgcgaca ttccggccga cgcctattgc ctgatcggcg tcacgcaggt
     1321 cgacctctac cccggtccgg cgtggaactt cgtgttcggc gaggccctgc tgtacgagcg
     1381 cgtcggcgtg tacagctttg cccgctacga cccgaaattc tacgacgagc cgcggaacgc
     1441 cggcacgccg gtgcagatcc tccgccgcgg cttcaagctg atggcccacg agatcgggca
     1501 catgtttgcg atcgaccact gcacacatag ccactgtgtc atgaatggca ccaacaacct
     1561 cgaggagacc gaccgcagcc cgctgcaccc gtgcccgatg tgcctgcgca agctccacga
     1621 gatcgtcggc ttcgacgtcg ccgcgcgtga gcgcgagctc ggcgaattct accgcgcgct
     1681 cggcctcacc agcgaggccg acgactgcga ccgccgcctc gcgcggatcg tcggctgacc
     1741 cgcgccgggc ccgtcgcggc agctagacgc agagcccggt gccggcgtcg caggtcttgc
     1801 cctcctcgca ggtcgccgga tcggccgggt cgcagtcgag ctcgcagaag ctcaggatgc
     1861 aggcgcagtc catcctgacg acgcaatggg agttgtcgtc gcagctgtcg ccgccgacgc
     1921 ccgtcgacgc gtcctcgtcg accgtgcact cctcctcgct cgcgcagctc gcgacgcacg
     1981 ggttctcccc gcctgacccg gtgaccacgt cggtgtcctg agtcgtcgta ccggtgtcct
     2041 gagtcgtcgt accggcgccg gtcgtcgtcg tatcggtgcc gggcatcgtc gtgcccgtgc
     2101 cggtcaccgt cgtgccgctg ctcgtcgcgt ccgtgtcgcc ggtcgtctcg gtgccgggcc
     2161 cgtccttgcc gccgcacgcg gcgagcatca ccagcacaca gaacgacgaa agtcggagga
     2221 tggaattatg tcgcgcgaag atgctcataa acaatgtcct gaaggccgca aaggtgccac
     2281 aaacggcgtc cgcgcgcccg cacgatccgc ggggggcgcg aaatcagctc gctcccgctt
     2341 gacatattcc gaaaacggaa tataaggcag gaatggctcg caacgccgcc acgacagatg
     2401 ttttcgccgc ggtcgcggag ccgagccggc gccggatcct cgagctgctc gccccgggtg
     2461 agcggccggt gaacgacgtc gcgcggctgc tgcacctgac ccagccgacg gcctccaaac
     2521 atctgaaagt tttgaaggat gtcggcctcg tgcacgtgcg cgaggccggg cagcagcgct
     2581 tttacagcgt caacggtcgg gcgctcgagc cgatccacgc gtggtcccgg caattcgagg
     2641 cgtactggaa cgacagct
//
LOCUS       DASWNJ010001522         4701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_378804, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001522 DASWNJ010000000
VERSION     DASWNJ010001522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378804"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VGB85_25315"
     CDS             <1..385
                     /locus_tag="VGB85_25315"
                     /inference="COORDINATES: protein motif:HMM:NF040147.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137438.1"
                     /translation="GRAIVREAPTVQRSTGDLGTQLPYVLLVGRLAHYLQRVQREHVG
                     QWDDRNTLQRELDLWLRQYVADMDDPPPEVRARKPLRRASIALAPVDGHPGWHRCHLT
                     VVPHLTHLGRPLTLELIGRLDLGPR"
     gene            393..2678
                     /gene="tssI"
                     /locus_tag="VGB85_25320"
     CDS             393..2678
                     /gene="tssI"
                     /locus_tag="VGB85_25320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HEY0137439.1"
                     /translation="MPDHPAPHQKHTLLPEARYTFEVSDQVADVHVVRVALYEILSEP
                     YRLIVDLLCEDHEFSADDLLGADCRLTIARDESPARDVHGLVLRVEQRGRRDRRQALR
                     LEIGPALALLAHQVDTRAWQQLSAPQIALDVLEDRLSALGRRVRLDLDTAAYTPREYC
                     VQYRESDLDFVLRLLHDEGIAFRFDHDSHADSETLVLFDTNTRCPARAPVPFNRHETA
                     LSAEQSVERLERARQLGVTGVTQRDWIPLIAGESPHTYTRCARDDRRRERIVYDHDDR
                     RIEADDAGTRARRKLDRMTVLRDACTGAGDVLEFTPGLRFELAGHPESARDRSYLLLS
                     VEHHGAVPDADRLGDDLDAPRYTNSFTCVPDDVPFRPQLPERLRRPRVHGPHTAIVVG
                     PEGEEIHTDEHGRIKLRFHWDRDSPADDRASCWVRVAQTWAGAGWGALFIPRVGMEVL
                     VEFIDGDPDRPLVVGCVYNSHNTPPVALPDDKTCSALTSESSPGGGLANQIRLEDGRG
                     RESLTLKTRRDLHTTAGHDHTATIAHDHTIKIGANQTRLIGANQVTTVQAHCDLTVAD
                     GDLTTTVLAGKQLTDIHGDQSTIVRAGNVQLEVECGNLRTRAHGMVQTTSTNANIHIK
                     AHGALYAESETDDLILDAKHEVRAQSQTASLRLKAATDIQLTSDSDEIAARAHTNIKL
                     SAMTGDVSATAAGNLLLHADAAAQLTADKITLEASHTLELRVGNSSIVLSPGSIEIKS
                     PMITSAAVGEHLISGALIRIN"
     gene            2691..3680
                     /locus_tag="VGB85_25325"
     CDS             2691..3680
                     /locus_tag="VGB85_25325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137440.1"
                     /translation="MPQARLRALQQILLTLVEHPEPRALVVACTDHEVFYIAHLLGQL
                     DEESPADRFCVLSDPFLKNAEDYVDRLETVAQHAIDLPVPRSCDPTPGSRLLALLDHL
                     LADLPPGDHRLVVALLPAEIHDRPGFSALVEVLLAAPLDPRLRLVLRDDRTAPRHFYT
                     AAQSASEQLFAYTFSLPPELLLADLLATTHDRNRPPDERALALIQLACQDLGHGRHTD
                     SLARCAAVARLPACAALQALALAIQADALRREGDSDAALATGVAALRLAVETNTLPVV
                     QHAALALGDLTRELGRITEAVACFELAERAASFNPAVAAHARALRSALTETPC"
     gene            3674..>4701
                     /locus_tag="VGB85_25330"
     CDS             3674..>4701
                     /locus_tag="VGB85_25330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HEY0137441.1"
                     /translation="MLIFDPLSSFIPPNVELLLPDDLAASQDVARGPTPKLSDIAPAV
                     RALTTRLTAPLLPEKAADESRLDNAVRESLGALGALKVPVQSLETAFAFATASIGAAF
                     ELPAASIAIKSLHFGLPHVHAHPPAFPNPLPSIGSLFLGGTQSVLINNHPAARAGDLG
                     IAITCGSFTPFFKVTTGSSKVFFGPNRAARSLDLTTFCKASSSSKSWVDKLPDHDSTA
                     NIANAGVKSVAGMSKTMATDGAARRESDKAMAQSLAAESEGHALSAAVQAKQALVGAA
                     VKVLSELVGKDGAAGPVFPGMLSVLGTNVLIGGFPTPPTLKFLVGLRKGLSASSVRFA
                     AGVTAGVY"
BASE COUNT          730 a         1996 c         1302 g          673 t
ORIGIN      
        1 cggccgcgcg atcgtccgcg aggccccgac cgtccagcgc agcaccggtg acctcggcac
       61 ccagctcccc tacgtcctgc tcgtcggtcg cctcgcccac tatctccagc gcgtacagcg
      121 cgagcacgtc ggacagtggg acgaccgcaa cactttgcag cgtgagctcg acctgtggct
      181 gcggcagtac gtcgccgaca tggacgaccc gccccccgag gtccgcgccc gcaaacccct
      241 gcgccgcgcc tcgatcgcgc tcgcccccgt cgatggccac cccgggtggc accgctgcca
      301 cctcaccgtc gtcccccacc tcacccacct cggccgcccg ctgaccctcg agctcatcgg
      361 ccgcctcgac ctcggtccgc gctagagatc ccatgcccga ccatcccgcc ccgcatcaaa
      421 agcacaccct cttgcccgag gcccgctaca ccttcgaggt aagcgatcaa gtcgccgacg
      481 tgcacgtcgt ccgcgtcgcg ctctatgaaa tcctctccga gccctaccgc ctgatcgtcg
      541 acctcctctg cgaggaccac gaattctccg cggacgacct gctcggcgcc gactgccgcc
      601 tgacgatcgc ccgcgacgag tcccccgccc gcgacgtcca cggcctcgtc ctgcgcgtcg
      661 agcagcgcgg ccgtcgtgac cgccgccagg ccctgcgcct cgagatcggc ccggccctcg
      721 ctctgctcgc ccaccaggtc gacacccgcg cctggcagca gctctcggcc ccccagatcg
      781 cgctggatgt cctcgaggac aggctctccg cgctcggccg ccgcgtccgc ctcgacctcg
      841 acaccgccgc ctacaccccg cgcgagtatt gcgtgcagta ccgtgagagc gacctcgact
      901 tcgtcctgcg cctgctccac gacgagggca tcgcctttcg cttcgaccat gacagccacg
      961 ccgacagcga gaccctcgtc ctcttcgaca ccaacacccg ctgccccgcc cgcgcccccg
     1021 tccccttcaa ccgccacgag accgccctgt ccgccgagca gtccgtcgaa cgcctcgagc
     1081 gtgcccgcca gctcggcgtc accggcgtca cccagcgcga ctggatcccg ctgatcgccg
     1141 gcgagtcgcc ccacacctac acccgctgcg cccgcgacga ccgccgccgc gagcgcatcg
     1201 tctacgatca cgacgaccgt cgcatcgagg ccgacgacgc cggcacccgc gcccgccgca
     1261 agctcgaccg catgaccgtc ctgcgcgacg cctgcaccgg cgccggcgac gtcctcgagt
     1321 tcacccccgg cctgcgcttc gagctcgccg gccaccccga gagcgcccgc gaccgcagct
     1381 acctgctgct gagcgtcgag caccacggag ccgtcccgga cgccgaccgc ctcggcgacg
     1441 acctcgacgc cccgcgctac accaacagct tcacctgcgt cccggacgac gtcccgttcc
     1501 gcccacagct ccccgagcgc ctgcgtcgtc ctcgcgtgca cggcccgcac accgcgatcg
     1561 tcgtcggccc cgagggcgag gagatccaca ccgatgagca cggaaggatc aagctccgct
     1621 tccactggga tcgcgacagc cccgccgacg accgcgcctc ctgctgggtg cgcgtcgccc
     1681 agacctgggc cggcgccggc tggggcgccc tcttcatccc gcgcgtcggc atggaggtgc
     1741 tcgtcgaatt catcgacggc gaccccgacc gcccgctcgt cgtcggctgc gtctacaact
     1801 cgcacaatac cccgcccgtc gccctccccg acgacaagac ctgcagcgcc ctgacctccg
     1861 agtcgagccc gggcggcggc ctcgccaacc agatccgcct cgaggacggc cgcggtcgcg
     1921 agtccctgac cctcaagacc cgtcgtgacc tccacaccac cgccggccac gaccacaccg
     1981 ccaccatcgc ccacgatcac acgatcaaga tcggcgccaa ccaaaccagg ctcatcggcg
     2041 ccaaccaggt caccaccgtc caggcccact gcgacctcac cgtcgccgat ggagatctga
     2101 cgaccacggt cctcgccggc aagcagctca ccgacatcca cggtgaccag agcaccatcg
     2161 tccgcgccgg caatgttcag ctggaggtcg agtgcggcaa tcttcgcacc cgcgcgcatg
     2221 gaatggtcca gaccacctcg acgaacgcca acattcacat caaggcccat ggcgccctgt
     2281 atgccgaatc cgagaccgac gacctgatcc tcgacgcaaa gcacgaggtc cgcgcgcaat
     2341 cccaaactgc ctcgctccgg ctcaaggcgg cgaccgacat ccagctgacc tcggactccg
     2401 acgagatcgc cgcgcgggca cacacaaata tcaagctcag cgccatgacg ggtgatgtgt
     2461 ccgcgaccgc cgccggcaac ctcctactcc acgcagacgc cgcggcccag ctcaccgccg
     2521 acaagatcac cctcgaggcc agccacaccc tcgaactccg cgtcggtaac agctcgatcg
     2581 tcctgagccc cggatcgatc gagatcaagt cgccgatgat caccagcgcc gccgtcggtg
     2641 agcacctgat ctccggcgcg ctcatccgca tcaactgacc cggtgcaccg atgccgcagg
     2701 cccgccttcg cgctctccag cagatcctcc ttaccctggt ggagcacccc gagccgcgcg
     2761 cgctcgtcgt cgcgtgcacc gaccacgagg tgttttatat tgcccacctc ctcggccagc
     2821 tcgacgagga gagccccgcc gaccgcttct gcgtcctctc cgaccccttt ttaaaaaatg
     2881 ccgaggatta cgtcgaccgg ctggagacgg tggcacaaca tgcgatcgat ctgccggtcc
     2941 cccggagctg cgatccaacc cccggatcgc ggctcctggc gctgctcgac cacctgctcg
     3001 ccgacctccc cccgggagat caccgcctgg tcgtcgcgct cctgcccgcc gagatccacg
     3061 atcgcccggg cttctctgcg ctcgtcgagg tgctgctcgc cgccccgctc gacccacgcc
     3121 tgcgcctcgt gctgcgcgac gaccggaccg ccccgcgaca tttttatact gcagcacagt
     3181 ccgcctcgga gcagctgttc gcctacactt tctccctccc gcccgagctc ctgctcgccg
     3241 acctcctcgc caccacccac gaccgcaacc gaccgcccga cgagcgcgcg ctggccctga
     3301 tccagctcgc ctgtcaggac ctcggtcacg gtcgacacac cgattcgctg gctcgctgcg
     3361 ccgcggtcgc ccgcctgccg gcctgcgccg cgctccaggc cctcgccctc gcgatccagg
     3421 ccgacgcgct gcgccgcgaa ggcgacagcg acgccgccct cgccaccggt gtcgcggccc
     3481 tgcggctcgc cgtcgagacc aacaccctcc ccgtcgtgca acacgccgcg ctggcgctcg
     3541 gggacctcac ccgcgagctc ggccgcatca cggaggccgt cgcgtgcttc gagctcgccg
     3601 agcgcgctgc atccttcaac ccggcagtgg cggcgcacgc ccgggccctg cgaagcgcgc
     3661 tcacggagac cccatgctga tcttcgaccc cctctcctcg ttcatccccc cgaacgtcga
     3721 acttctgctc ccggacgacc tcgccgcctc acaggacgtc gcgcgaggac cgacgcccaa
     3781 actctcggac atcgcgccgg cggtccgcgc cctgaccact cgcctcacag cgccgctcct
     3841 cccggagaag gccgccgacg agtcccggct cgacaacgcc gtccgcgaga gcctgggcgc
     3901 gctcggagcc ctcaaggtcc cggtccagtc gctcgagacc gccttcgcct tcgcgaccgc
     3961 gtcgatcggc gccgcgttcg agctgcccgc cgcgagcatc gccatcaaat cccttcactt
     4021 cgggctgccg catgttcacg cgcatccgcc cgctttcccc aacccgctcc cgagcatcgg
     4081 ctccctgttc ctcggcggga cccagtccgt gctgatcaac aaccacccgg ccgcccgcgc
     4141 cggcgacctc gggatcgcga tcacctgcgg ctccttcact ccctttttta aagtgaccac
     4201 gggctcgagc aaggtcttct tcggcccgaa ccgcgccgcg cgctccctcg atctcacgac
     4261 cttttgtaag gcatcctcgt cgagcaaatc ctgggtcgac aagctccccg accacgactc
     4321 caccgccaac atcgccaatg ccggggtgaa gtccgtggca ggcatgagca agaccatggc
     4381 gaccgacggc gcggcccgcc gcgaatccga caaagccatg gcgcagtcgc tcgcggcgga
     4441 gagcgagggc cacgcgctct ccgccgccgt ccaggccaag caggccctcg tcggcgccgc
     4501 cgtcaaggtc ctctcggagc tcgtcggcaa ggatggtgcg gccggaccgg tcttccccgg
     4561 gatgctgagc gtgctgggta ccaacgtcct gatcggtggc tttcccacgc ctccgaccct
     4621 caagttcctc gtcggactca gaaagggcct gtccgcgagc agcgtccgct tcgccgccgg
     4681 ggtcacggcc ggcgtctatt g
//
LOCUS       DASWNJ010001523         2780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_204565, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001523 DASWNJ010000000
VERSION     DASWNJ010001523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204565"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VGB85_25335"
     CDS             <1..646
                     /locus_tag="VGB85_25335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137442.1"
                     /translation="GSMFAVSIEVAGEDRDRVLSLPSGTPAVSPPRTHVQRELDDGGL
                     WLADWGDVQRESYWEPFSGELHTLYDHFQLPPRYRRAAADGLELINLPQAAGFTETPP
                     GRLVLLPWDGSKGEVLAEDIYPWYRRLADGRIITTRPTSDGFKGGDPVDLVLITGDSE
                     RVIDRQVILLGPLLAGDGEPDKGYFRIIGDLLLYAVRDDAGDRTGVWAVSLSQL"
     gene            complement(656..1954)
                     /locus_tag="VGB85_25340"
     CDS             complement(656..1954)
                     /locus_tag="VGB85_25340"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEY0137443.1"
                     /translation="MRFSRRRFLGGAGVLVALPAFGMIRPRHAASEPQTPVKRFLVYF
                     LPNGRVPATWVPPQVGTEFSFPPALAPLAALKGDMIALSGLYHTSAKMSSGTGDHALG
                     TGSILTCVPHPDGGLHGDISMDQALVKALAPATRFPSLQWGAGEPMACDFGASCSYTQ
                     SISWAGPKNPLAPVINPLTAFNQLFAGSNEGATAMEQEIRRNSLKSVLDYVLSDAESL
                     STELGAADKIKLDEYFTSVRELETRLTSPAGACMSGPAPADGLDYPGRVTAFNDLIAL
                     AFQCNQSQVITFMIENGLSGRGHPFIDAPGGHHGLTHGAGVDAPGQLLRLETWQAQQA
                     ANLATKLKGMLDPDGTPILDNTAMLLLPDMGDGGLHDHTNLSPVILGRLGGALNVNQS
                     IHYASEPLGNLYVSLLRAFGVDTTTFGIDGVAPLAGVNLT"
     gene            complement(1959..>2780)
                     /locus_tag="VGB85_25345"
     CDS             complement(1959..>2780)
                     /locus_tag="VGB85_25345"
                     /inference="COORDINATES: protein motif:HMM:NF019256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HEY0137444.1"
                     /translation="LSTRALAYHAPDPTVFPGFDEPLRAAMAAEAELFFSDFLLNGEP
                     VGAMMNPDFGYLNDRLAQHYGLPLPKSAEMTRVQLADDQRRGLMMQGAWLTATSASDR
                     TSPVVRGRWILEQVLCTPMPPPPPDIPPLMKPEPGETVRETLAKHRENPVCAGCHDLL
                     DPAGLGMEKFDGIGALRVLENGQPIDVSGGIPPQIAFEGASELAALLGDDPRFTQCLT
                     EKLYSYALGRKKVPKDFPYLDDIDAALAESSGTLIDIIDLIATSPAFRMRPKKVE"
BASE COUNT          394 a          875 c         1085 g          426 t
ORIGIN      
        1 cggctcgatg ttcgcggtgt cgatcgaggt cgcgggcgag gaccgggacc gcgtgctgtc
       61 gctgccctcg ggcacaccgg ccgtgtcgcc gccccggacc cacgtgcaac gcgagctgga
      121 cgacggcggg ctgtggctgg cggactgggg ggacgtccag cgcgagtcct actgggagcc
      181 gttttcgggc gagttgcaca cgctgtacga ccacttccag ctgccgcccc ggtatcgccg
      241 cgcggccgcg gatgggctcg agctgatcaa cctcccgcag gctgccgggt tcacggagac
      301 cccgcccggc aggctggtcc tcctgccgtg ggacggctcg aagggggagg tgctggccga
      361 ggacatctat ccctggtacc ggcggctcgc cgacggccgc atcatcacca cgcgcccgac
      421 gagcgacggc ttcaagggcg gcgatccggt cgacctggtg ctgatcaccg gggacagcga
      481 gcgagtgatc gaccgccagg tcatcctgct cgggccgctg ctcgccggcg acggcgagcc
      541 cgacaagggg tactttcgca tcatcggcga cctgctgctc tacgcggtgc gcgacgacgc
      601 gggcgaccgc acgggggtgt gggcggtcag cctgtcgcag ctgtgagcgg ccgcgctacg
      661 tcaggttgac accggcgagc ggggcgacgc cgtcgatgcc gaaggtcgtg gtatcgacgc
      721 cgaaggcgcg cagcagcgag acgtagaggt tgcccagcgg ctcgctcgcg tagtggatcg
      781 actggttgac gttgagggcg ccaccgaggc gaccgaggat caccggcgag aggttggtgt
      841 ggtcgtgcag gccgccgtcg cccatgtccg ggagcagcag catcgcggtg ttgtcgagga
      901 tcggggtgcc gtcggggtcg agcatgccct tgagcttggt cgccaggttg gcggcctgct
      961 gggcctgcca ggtctcgagg cgcagcagct ggccgggggc gtcgacgccg gcgccgtggg
     1021 tgaggccgtg gtggccgccc ggggcgtcga tgaaggggtg cccgcgaccg ctgagcccgt
     1081 tctcgatcat gaaggtgatc acctggctct ggttgcactg gaacgcgagc gcgatcaggt
     1141 cgttgaaggc ggtgacgcgg ccggggtagt cgaggccgtc ggcgggggcc gggccggaca
     1201 tgcaggcgcc ggccggcgaa gtcaggcggg tctcgagctc gcgcaccgag gtgaagtact
     1261 cgtcgagctt gatcttgtcg gcggcgccga gctcggtcga caggctctcg gcgtcgctga
     1321 gcacgtagtc gagcacgctc ttgagcgagt tgcggcggat ctcctgctcc atcgcggtcg
     1381 cgccctcgtt ggacccggcg aacagctgat tgaaggcggt cagcgggttg atcaccggcg
     1441 cgagcgggtt cttcggcccg gcccacgaga tgctctgcgt gtagctgcac gaggccccga
     1501 agtcgcaggc catcggctca ccggcgcccc actgcagcga ggggaagcgg gtcgctggcg
     1561 cgagcgcctt gacgagcgcc tggtccatcg agatgtcacc gtgcaggccg ccgtccgggt
     1621 gcggtacgca ggtgaggatc gacccggtgc cgagcgcgtg gtcgccggtg ccggacgaca
     1681 tcttggcgct ggtgtgatag aggcccgaga gcgcgatcat gtcgcccttg agggcggcga
     1741 gcggcgcgag ggccggcggg aacgagaact cggtgccgac ctgcgggggc acccaggtcg
     1801 cgggcacgcg gccgttgggc aggaagtaca cgaggaagcg cttgaccggc gtctgcggct
     1861 cggaggccgc atggcgcgga cggatcatac caaaggcggg cagggcgacc aggacaccgg
     1921 cgccgccgag gaatctgcgt cgtgagaatc gcatcagttt attccacctt cttggggcgc
     1981 atccggaacg ccggcgaggt cgcgatcagg tcgatgatat cgatcaaggt gccgctgctc
     2041 tccgcgagcg cggcgtcgat gtcgtcgagg tacgggaagt ccttggggac cttcttgcgc
     2101 ccgagcgcgt agctgtacag cttctcggtc agacactggg tgaagcgcgg gtcgtccccg
     2161 agcagcgcgg cgagctcgga ggcgccctcg aacgcgatct gcggcgggat gccgccgctg
     2221 acgtcgatcg gctgaccgtt ctccaacacg cgcaatgcac cgatgccgtc gaacttctcc
     2281 atgccgaggc cggcggggtc gagcaggtcg tgacagccgg cgcagaccgg gttctcgcgg
     2341 tgcttggcga gggtctcgcg cacggtctca ccgggctcgg gcttcatcag cggcgggatg
     2401 tccgggggcg gcggcggcat cggggtgcac agcacctgct cgaggatcca ccgcccgcgt
     2461 acgaccggtg aggtgcggtc gctcgccgag gtcgcggtca gccacgcgcc ctgcatcatg
     2521 aggccgcgcc gctggtcgtc ggccagctgc acgcgcgtca tctcggcgct cttgggcagc
     2581 ggcaggccgt agtgctgcgc gaggcggtcg ttgaggtagc cgaagtcggg gttcatcatc
     2641 gcgccgaccg gctcgccgtt gagcaggaag tcgctgaaga acagctcggc ctcggcggcc
     2701 atggcggcgc gcaggggctc gtcgaagccg gggaagacgg tcgggtcggg cgcgtggtag
     2761 gcgagcgcgc gggtcgagag
//
LOCUS       DASWNJ010001524         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_239126, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001524 DASWNJ010000000
VERSION     DASWNJ010001524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239126"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VGB85_25350"
     CDS             <1..273
                     /locus_tag="VGB85_25350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137445.1"
                     /translation="IYCSELVRLAYAAIGLELGESTPAAALGLHTAPVQALLRKRWRA
                     HPSCAGAPDLAACMPALGRLEIVTPVSLRRDRQLTLVASDYPAPLR"
     gene            315..>1501
                     /locus_tag="VGB85_25355"
     CDS             315..>1501
                     /locus_tag="VGB85_25355"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEY0137446.1"
                     /translation="MSAPRPLADVARDLGIDPAHRSYLPYGHDFAKLDTVAAGDGAHT
                     GKLVVVSAVTPTPLGEGKTVTSISLSMGLNKIGEPCIVCLRQPSLGPVFGSKGGGAGG
                     GRAQVIPADAINLGMTGDGHAIGAAHNLLAAMLDAHLRHGNELRLDPVRPTWPRVSPV
                     NDGALRKLIVGLGGPLNGVPRETFFEINEASEVMAIVALARDRADLRQRLGAIVVGQD
                     VAGNPVRADQLGAAGAMSVLLRDAVFPTLTQTSEGTPALIHAGPFANIAHGNNSIVAT
                     RFALTRAPWVVTECGFGSDLGLEKFMHIKARAAGLQPACVVLVCTVRALKVHAGVAKV
                     AVGKPLDQVVSVPDVAAVERGAANLRHHLRITQGFGVKVVVALNHFPSDGPDEVAVVE
                     RIAR"
BASE COUNT          196 a          624 c          488 g          193 t
ORIGIN      
        1 atctattgca gcgagctggt ccggctcgcc tacgccgcga tcggcctcga gctcggcgag
       61 tcgacccccg cggccgcgct cggcctgcac accgcccccg tgcaggcatt gctgcgcaag
      121 cgctggcggg cccacccgag ctgcgcgggc gcgcccgacc tcgccgcctg catgccggcc
      181 ctcgggcgcc tggagatcgt caccccggtc agcctgcgcc gcgatcgcca gctcaccctc
      241 gtcgccagcg actacccggc gccgctgcgc tgagcggctg gtcgcgtccc cccggggcgt
      301 ggcaaagtcg ccgcatgtcc gcaccacgcc cgctcgccga cgtcgcccgg gacctcggca
      361 tcgaccccgc gcaccgcagc tacctcccgt acggccacga cttcgccaag ctcgacaccg
      421 tcgccgccgg cgacggcgcg cacaccggca agctcgtggt cgtctccgcg gtcacgccga
      481 cgccgctggg cgagggcaag accgtcacct cgatcagcct cagcatgggc ctcaacaaga
      541 tcggcgagcc gtgcatcgtg tgcctgcgcc agccctcgct cggcccggtg ttcggcagca
      601 agggcggcgg cgccggcggt gggcgggccc aggtcatccc ggccgacgcg atcaacctcg
      661 gcatgaccgg cgatggccac gcgatcggtg ccgcgcacaa cctgctcgcc gcgatgctcg
      721 acgcccacct gcgccacggc aacgagctgc gcctcgaccc cgtgcgcccg acctggccgc
      781 gcgtcagccc cgtcaacgac ggcgcgctgc gcaagctgat cgtcggcctc ggcggcccgc
      841 tcaacggcgt cccgcgcgag accttcttcg agatcaacga ggcgagcgag gtcatggcga
      901 tcgtcgcgct ggcccgcgac cgcgccgacc tgcgccagcg cctcggcgcg atcgtcgtcg
      961 gccaggacgt cgccggcaac cccgtgcgcg ccgaccagct cggcgccgcc ggggcgatga
     1021 gcgtgctgct gcgcgacgcc gtgttcccga ccctgaccca gacctccgag ggcacccccg
     1081 cgctgatcca cgccggaccg ttcgccaaca tcgcccacgg caacaactcg atcgtcgcca
     1141 cccgcttcgc cctgacccgc gccccctggg tcgtcaccga gtgcggcttc ggcagcgacc
     1201 tcggcctcga gaagttcatg cacatcaagg cgcgcgccgc cggcctgcag cccgcgtgcg
     1261 tcgtgctcgt gtgcaccgtc cgcgcgctca aggtccacgc cggcgtcgcc aaggtcgccg
     1321 tcggcaagcc gctcgaccag gtcgtgtcgg tgcccgacgt cgcggccgtc gagcgcggcg
     1381 ccgccaactt gcggcaccac ctgcggatca cccagggctt cggcgtcaag gtcgtcgtcg
     1441 cgctcaatca cttcccgagc gacggccccg acgaggtcgc cgtcgtcgag cgcatcgccc
     1501 g
//
LOCUS       DASWNJ010001525         3387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_166788, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001525 DASWNJ010000000
VERSION     DASWNJ010001525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166788"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="VGB85_25360"
     CDS             <1..902
                     /locus_tag="VGB85_25360"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0137447.1"
                     /translation="KKHALDWLAREVATRILGAPLTLPAERDASMITYEALRARDSER
                     GRKRRPSRESRAAGDRERAPSQEQGDIRYPAPAEEFLDFLTDVELAASPLNAPDSPFG
                     RLASRIEERIQAGHRTGYEATALGGRELALEVSDTLRIDLYDASSPIKQLAPLLLYLR
                     YRAAARQMLIIDEPELNLHPEGQAELLEALAMLSNLGVTVLLTTHSPYIMAHLNTLVA
                     ADTGARPRRLRQAEHLFMGDPAAFLARDAVSAYEMRTDGLVALADPAYGIRCDTLSDV
                     SAELQRRYFAVVKEPKRRAKRKT"
     gene            1062..1823
                     /locus_tag="VGB85_25365"
     CDS             1062..1823
                     /locus_tag="VGB85_25365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137448.1"
                     /translation="MKRPEPHEVAALLDRHGIVPSPQCPPELLNAHRTLIYFILRAFD
                     SDARAAAGMLAAVVEWYRRYDELAGAQPVEKDFDYPDGKRRLRLACGARCNHCCTAPV
                     SAIGPEVALIAAYIRAKFTAEQRASLAARIAARLAVRNDAANGPQMCPLNVDGKCSVY
                     AMRPLNCRKWHSFDESACRKAFLHDDRTVLIPRAGVRADASGLLWQSAVAAFSAVGLK
                     IAELDFIPALEVALADERVAARYAAGEPVFADVER"
     gene            1863..2003
                     /locus_tag="VGB85_25370"
     CDS             1863..2003
                     /locus_tag="VGB85_25370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137449.1"
                     /translation="MPTPRRVLLSLACLVLTACPKPGPPPTSAGLSTLSLAPPLALHP
                     RR"
     gene            complement(2013..3194)
                     /locus_tag="VGB85_25375"
     CDS             complement(2013..3194)
                     /locus_tag="VGB85_25375"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEY0137450.1"
                     /translation="MFPAILLAAVTWLAPAAGPVGDPALQRAYAVLARVPLIDGHNDL
                     PWTVREYKDGPRDVVAYDLHTPRPGHTDLPRLRAGRVGGQFWSVYVPGDRAAEGLVRL
                     QLEQIDLARRMIARYPDDMTLVLGADELERAFRGGKIASLLGAEGGHTIADSLAVLRT
                     YHALGVRYLTLTHNVHTRWADCAALPARHGGLTRFGEEVVREMNRLGMLVDLSHVSDA
                     TMDDALRVSAAPVIFSHSSARALTDVPRNVPDAILGRVAKNGGIVMVTFLPAYVSQEV
                     ADHRKVKQPRQPAPRATLAQVADHIEHVRRVAGADHVGIGGDFDGMSSAPVGLEDVSK
                     YPQLFAELVRRGWSDADLEKLAGRNLLRVLRAAEAVAREQATRPASLATIDQLDGPPA
                     R"
BASE COUNT          515 a         1252 c         1129 g          491 t
ORIGIN      
        1 acaagaagca cgcgctcgac tggctggccc gggaagtcgc aaccaggatc ctcggggctc
       61 cgctcacgct gccggcggag cgtgacgcat ctatgatcac gtacgaggcc ctcagggcgc
      121 gtgacagcga acgtggtcgc aagcggcgtc catcgcgtga atcacgagca gccggcgacc
      181 gcgagcgggc gccctcgcag gagcagggtg acattcgtta tcccgcgccg gcagaagagt
      241 tcctcgactt cctcacggac gtcgagctgg ccgcgagccc gctcaacgct ccagattctc
      301 cgttcggccg gcttgcttca cgcatcgagg agcgcatcca ggccgggcac cgaaccggct
      361 acgaggccac cgcgctcgga ggccgcgagc tcgcgctcga ggtcagcgac actctgcgca
      421 tcgacctgta cgacgcgtcg tcgccgatca agcagctggc gccgctcctc ctgtatctgc
      481 ggtaccgggc ggcggcgcgg caaatgttga tcatcgacga accggagctc aacctgcacc
      541 cggagggtca ggccgagctc ctcgaggcgc tggcgatgct gagcaacctc ggtgtgaccg
      601 tgttgctgac cacacacagc ccgtacatca tggcgcacct caatactctg gtcgccgcgg
      661 ataccggcgc tcggccgcga cgactgcgcc aggcggagca cctgttcatg ggcgacccgg
      721 cggccttcct cgccagggat gcggtcagcg cgtacgagat gcggactgac gggctggtcg
      781 cgctcgcaga cccggcgtac gggatccggt gtgataccct gagcgacgtg tccgccgaat
      841 tgcagcgccg ctacttcgcc gtcgtgaagg agccgaagcg gcgtgcgaag cgaaagacgt
      901 gacggcgagc gaagccgcgg ccaggcttcg ccacggcgcg gggtcgtcga gaccctgacg
      961 gtgcgggaga cattgccatt gggcgcgatc tggacacgat atggggacga acgaatgaac
     1021 cgaaactatc gctcgccgac acgccgcggg ggacagcgcg catgaagcgg cccgagccac
     1081 acgaggtcgc cgcgctgctg gacaggcacg gcatcgtacc cagcccgcag tgcccacccg
     1141 agctgctcaa cgcccacagg accctgatct atttcatcct gcgcgcattc gactcggacg
     1201 cgcgcgcggc ggccggcatg ctcgccgccg tggtcgagtg gtatcgccgg tacgacgagc
     1261 tggcgggcgc gcagccggtc gagaaggact tcgactatcc cgacggcaag cgtcgcctgc
     1321 gcctcgcatg tggtgccagg tgtaatcatt gttgcaccgc gccggtctcg gcgatcggcc
     1381 cggaggtcgc gctgatcgcc gcctatatcc gggccaagtt caccgcggag caacgcgcct
     1441 cgctcgcggc ccggatcgcg gcccggctcg cggtccgcaa cgacgccgcg aacggccccc
     1501 agatgtgccc gctcaacgtc gacggcaaat gctcggtgta tgcgatgcgc ccgctcaatt
     1561 gtcgcaagtg gcactcgttc gacgagtcgg cctgtcgcaa ggcgttcctg cacgatgacc
     1621 gcaccgtcct gatcccgcgc gccggcgtgc gggccgacgc gtccgggctg ctgtggcaga
     1681 gcgcggtcgc ggcgttctcg gcggtcggcc tgaagatcgc cgagctcgac ttcatccccg
     1741 cgctggaggt cgcgctcgcc gacgagcgcg tcgcggcgcg ttacgccgcg ggtgagccag
     1801 tgttcgcgga cgtcgagcgc tagcgaggac gatcgcgtat cgtgcctgcc ggagctctca
     1861 tcatgcccac tccgcgccgc gtcctgctct ccctcgcctg cctcgtgctc acggcctgcc
     1921 ccaaacccgg tcctccgccg acgagcgccg gcctgtcgac cctgagcctc gcgccgccgc
     1981 tcgcgctcca cccacgtcga taggcgtacc cgtcagcgcg ccggcggccc gtcgagctga
     2041 tcgatcgtcg ccagcgaggc cggccgcgtc gcctgctcgc gcgcgaccgc ctcggccgca
     2101 cgcagcaccc gaagtaagtt gcgcccggcc agcttttcca gatcggcgtc gctccagccg
     2161 cgtcgcacca gctcggcgaa cagctgcgga tattttgaga cgtcctcgag cccgaccggc
     2221 gccgagctca tgccgtcgaa gtcgccgccg atgccgacgt ggtccgcccc ggcgacccgg
     2281 cgcacgtgct cgatgtggtc ggcgacctgg gccagcgtcg cccgcggagc cggctgccgc
     2341 ggctgcttca ccttccggtg atcggcgacc tcctgcgaga cgtaggccgg caggaacgtc
     2401 accatcacga tcccgccgtt cttcgccacg cgcccgagga tcgcgtcggg cacgttgcgc
     2461 ggcacgtcgg tcagcgcgcg cgccgacgag tgcgagaaga tgaccggcgc cgcgctgacg
     2521 cgcagcgcgt cgtccatcgt cgcgtccgag acgtgcgaca ggtcgaccag catcccgagc
     2581 cggttcatct cgcgcacgac ctcctcgccg aagcgcgtca gcccgccgtg gcgggccggc
     2641 agcgccgcgc agtcggccca gcgagtatgc acattgtgcg tcagcgtcag gtaacgaacg
     2701 ccgagcgcgt ggtacgtgcg cagcaccgcc agcgagtccg cgatcgtgtg cccaccctcc
     2761 gcgccgagca acgaggcgat cttgccgcca cgaaacgccc gctcgagctc gtccgcgccg
     2821 agcaccagcg tcatatcgtc gggatagcgg gcgatcatcc ggcgtgccag atcgatctgc
     2881 tcgagctgca agcgaaccag gccctcggcc gcgcggtcgc ctggcacata caccgaccag
     2941 aactgcccgc cgacccggcc cgcccgcagg cgcggcaggt cggtgtggcc cggcctcggg
     3001 gtatgcaggt cgtacgcgac cacgtcgcgc ggcccatcct tgtactcgcg cacggtccac
     3061 gggaggtcgt tgtgcccgtc gatcaacggc acgcgtgcca gcaccgcgta cgcccgttgc
     3121 agcgccggat ccccgaccgg tccggcggcg ggcgccagcc aggtcacggc cgccaggagg
     3181 atcgcaggga acacgcccca gggtacgcca ggtcgccgac gccgggcaat ggcgcggatc
     3241 cgcatgtctt cgcgacaaaa gccgtgtcag cgtccgccgt ctcgccgaca atgcaggcat
     3301 atgtcctcgc ctcgctcgcc gtcccggtcg ctgcggaccc tcgccgtctc ggccctcgcc
     3361 gccctcgccg ccctcgccgg ttgccac
//
LOCUS       DASWNJ010001526         1639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_378942, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001526 DASWNJ010000000
VERSION     DASWNJ010001526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378942"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1622)
                     /locus_tag="VGB85_25380"
     CDS             complement(<1..1622)
                     /locus_tag="VGB85_25380"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0137451.1"
                     /translation="MTLLAVLGEGGMATVYLASIGTGAAARLVAVKLLRPGLPDHDYR
                     TRFKDEAKLVLRLHHPNIVEVREAGDLHEQLFIVMALVEGRDLADIWDRCAAVGKAFP
                     VPLAVHIIREILRGLHYAHTFPGLGLVHRDVSPSNILIDWTGVVRLADFGLATSTLKG
                     SLTVPGVVFGKVGYMSPEQARHENLDGRADVYACGAVLWELLTGRPLRGGDGVDTEDV
                     SNFNAPPPSSYSSRVDAELDAIVSKALARDREDRYLSAHDFMEALGAWLARRGIVGNA
                     GAEQMAEFLAMLFGDAAVRERENRDALLGDINYQHAAMAPARAEEANKDTLGDRFEDD
                     EEFDLSLSEDEDGDGDRSDMKIIPLPERPERLDRAPGEKAPQARRPRAKEDIFGFNDN
                     PQNDVEHIEAGTIIADRYRVVSRIGRGGMGTVYLGEHTTVGRRVAIKVLHIRHDTPLA
                     LIERFRREARISALVRHRNVLEVYDTGSLADGSPYLVMERVNGENLSALLRRGPLSIA
                     AAIEVGSQLLSGLSAISDQGIVHRDIKPDNIFL"
BASE COUNT          239 a          546 c          597 g          257 t
ORIGIN      
        1 aggaagatgt tgtcgggctt gatgtcgcgg tggacgatgc cctggtcgga gatcgcggaa
       61 agcccactca acagctggct gccgacctcg attgccgccg cgatcgagag cggaccgcgg
      121 cgcagtagag cggacaagtt ctcgccgttg acgcgctcca tcaccaagta cggcgagcca
      181 tcggcgagcg agcccgtgtc gtagacctcg agcacgtttc gatgccgcac cagcgcggag
      241 atccgcgcct cccgtcggaa gcgctcgatc agtgccaagg gcgtgtcgtg ccggatgtgc
      301 aggaccttga tcgcgacgcg acgaccgacg gtcgtgtgct cgccgaggta cacggtgccc
      361 atgccgccgc gaccgatgcg actgacgacg cggtagcggt cggcgatgat cgtgccggcc
      421 tcgatgtgct cgacgtcgtt ctgcgggttg tcgttgaagc caaagatgtc ctccttggct
      481 cgcgggcgcc gcgcctgcgg ggccttctcg cccggcgcgc ggtcgaggcg ctcggggcgc
      541 tcgggcagcg ggatgatctt catgtcggat cgatcgccgt cgccgtcctc atcctccgag
      601 agcgagaggt cgaactcctc gtcgtcctcg aagcggtcgc ccagggtgtc cttgttggcc
      661 tcctcggcgc gggccggggc catcgccgcg tgttgatagt tgatgtcgcc gagcagcgcg
      721 tcccggttct cgcgctcgcg cacggcggcg tcgccgaaca gcatcgccag gaactcggcc
      781 atctgctcgg cgccggcgtt gccgacgatg ccgcgacgcg ccagccaggc gccgagcgcc
      841 tccatgaagt cgtgcgccga gaggtagcgg tcctcgcggt cgcgcgcgag cgccttgctg
      901 acgatcgcgt cgagctcggc gtcgacgcgg ctgctgtagc tgctcggcgg cggcgcgttg
      961 aagttcgaca catcctcggt gtcgacgccg tctccgccac gcagtggccg gccggtcagc
     1021 agctcccaca gcacggcgcc gcaggcgtag acatcggcgc ggccgtcgag gttctcgtgg
     1081 cgggcctgct cgggcgacat gtagccgacc ttgccgaaca cgacgccggg cacggtcagg
     1141 ctgcccttga gcgtggatgt ggcgaggccg aagtcggcga ggcgcacgac gccggtccag
     1201 tcgatcagga tgttcgaggg cgagacgtcg cggtggacga ggccgaggcc ggggaaggtg
     1261 tgggcgtagt gcaggccgcg caggatctcg cggatgatgt gcacggcgag cgggaccggg
     1321 aacgccttgc cgaccgcggc gcagcggtcc cagatgtcgg cgaggtcgcg accctcgacg
     1381 agcgccatca cgatgaacag ctgctcgtgc aggtcgccgg cctcgcgcac ctcgacgatg
     1441 ttcgggtggt gcaggcgcag gaccagcttg gcctcgtcct tgaaccgggt ccggtagtcg
     1501 tgatcgggca gacccgggcg cagcaacttg acggcgacca gacgagcggc ggcacccgtc
     1561 ccgatcgagg cgaggtacac cgtcgccatg cccccctcgc ccaggaccgc cagcagggtc
     1621 acgttgccca gctggcgcg
//
LOCUS       DASWNJ010001527         1773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_239136, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001527 DASWNJ010000000
VERSION     DASWNJ010001527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239136"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(432..1217)
                     /locus_tag="VGB85_25385"
     CDS             complement(432..1217)
                     /locus_tag="VGB85_25385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137452.1"
                     /translation="MSLSLVAVLAAGCDLGAVEVGGGFREGNEESGASDESGASDESG
                     TSDESGTSEESAGDASSDTASSGDEPPVDEGESDPTDEPPIDASGPLYEALTGCVFNY
                     VWSDSDPWGYGLGSGGSARGVDLGWVAAADATTLTFKPDDLGHLFGAPVYGVDYEPGK
                     DYAAVFTIELLEIDAVTGEYVLGGKAVSQYAVLTMPTLRIDPANPFVPNGDTAYTNGL
                     GVATMTSLVDDTEMSYPFQWGLWVNINGAENPACEPLLNAVWP"
     gene            complement(1280..>1773)
                     /locus_tag="VGB85_25390"
     CDS             complement(1280..>1773)
                     /locus_tag="VGB85_25390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137453.1"
                     /translation="LALLPAASAPPPPTPRPAPTVLAAARVVAPVPNDMSESPAPPAP
                     ELVAAAASPARSRPALARVRPPADDPLAREARQIGAAQRLLQDGRIDEALAMLDTHAA
                     EAPEGRLADEREALRAGARCGRGERDAALAAADLWFAARPQAPALATARALCRPKELS
                     TDE"
BASE COUNT          240 a          590 c          676 g          267 t
ORIGIN      
        1 tgcccacaca tggccggctc cgagcgcgac gcccgacgag cggtaacgtc accgctatcc
       61 gggttgcgag tctgctacga ggacagcgat gcagacccac ggcgacgctg tcgagcacct
      121 ccgggcccgc gacgtcgccg cgacgcggag ggcgtgggcg ctcggtgata ctgtcgtggt
      181 gccaatcggt gcgggacgac cggaccggaa ggtatcatcg tgtaccacgg tatgatgtgg
      241 ctggtgccgt gcgagggcgg atgggatgtg gtttcagccg gtgcaatcgc atgagcggcg
      301 gatccctttc gggtcgccgt tgaaggcgtg cgagacgggt cgcttcgcgt gctcgatcgc
      361 atgggcacgg cgggcatcat cgcggagctg cgaggtgggc cggccgaccc gaagggcccg
      421 ccgcgctcag gtcacggcca caccgcattg agcaggggct cgcaggccgg gttttcggcg
      481 ccgttgatgt tgacccagag gccccactga aacgggtagc tcatctcggt gtcgtcgacg
      541 agcgaggtca tggtggccac gccgaggccg ttggtatacg cggtgtctcc attgggcacg
      601 aacgggttgg cggggtcgat gcgcagcgtc ggcatggtca gcaccgcgta ctgggacacg
      661 gccttgccgc cgagcacgta ctcgccggtc acggcgtcga tctccagcag ctcgatcgtg
      721 aacacggccg cgtagtcctt gcccggctca tagtccacac cgtagacggg cgccccgaag
      781 agatggccga ggtcatcggg cttgaacgtg agggtcgtgg cgtccgcggc cgccacccag
      841 ccgagatcca cgccccgggc ggatccgccg gagcccaggc cgtagcccca ggggtcgctg
      901 tcgctccaca cgtagttgaa gacgcagccc gtgagggcct cgtacagcgg gccactcgcg
      961 tcgatcggcg gctcgtccgt ggggtcgctc tcaccttcgt cgaccggcgg ctcgtcgccg
     1021 ctgctcgcgg tgtcgctgct cgcatcgccc gcgctctctt cggacgtacc gctctcgtcg
     1081 gacgtaccgc tctcgtcgga cgcaccgctc tcgtcggacg caccgctctc ctcgttacct
     1141 tcacgaaagc cgccgccgac ctcgacggcg ccgagatcgc agcccgcggc gagcacggcg
     1201 accagggaca gggacaaagt gtggggatat ggatatcgag tcgtcatata agggtgagtg
     1261 gccgctcggg gccgattcgt cactcgtcgg tcgataattc tttcgggcga cacagcgcgc
     1321 gcgcggtcgc cagcgcgggc gcctgggggc gagccgcgaa ccaaaggtct gcggcggcga
     1381 gggccgcgtc gcgctcgccc cggccgcagc gggctccggc gcgcagggcc tcgcgctcgt
     1441 ccgcgagtcg tccctcgggg gcctcggcgg cgtgggtgtc gagcatcgcc agcgcctcgt
     1501 cgatacggcc gtcctgcagc agccgctggg cggcgccgat ctgccgggcc tcgcgcgcca
     1561 gcgggtcgtc ggcgggtggg cgcacgcgcg cgagtgcggg gcgtgaccgg gcgggggagg
     1621 ccgcggccgc gacgagctcc ggcgcgggtg gagctggcga ttcggacatg tcgttcggga
     1681 caggcgccac gacgcgggcc gcggcgagca cggtcggtgc ggggcgcggc gtcggcgggg
     1741 gtggggcact cgcggccggc aggagggcca ggc
//
LOCUS       DASWNJ010001528         2484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_592877, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001528 DASWNJ010000000
VERSION     DASWNJ010001528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592877"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..743)
                     /locus_tag="VGB85_25395"
     CDS             complement(3..743)
                     /locus_tag="VGB85_25395"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY0137454.1"
                     /translation="MIFLVLSVVTFVVSGLLFMWLERRWPARPTRALAGALALELPYV
                     LLSNALPGWAVGRLGAALHVTGPLPALPMFVQWLVVLAVTELTFYAVHRGMHTWPGLW
                     RLHAPHHAPAEIDWLAGFRKHTGEALLHGLAPLPVLVLLGPAVEVMLFHTLFGVVLTG
                     FTHLNSSIRLGWLESVLVTPRVHAWHHAVAVEDQRRNLAGKLTLLDRVFGSWNRVGGW
                     PAQLGLMNRAGARDDWRTTHGLRRGAPP"
     gene            complement(844..1404)
                     /locus_tag="VGB85_25400"
     CDS             complement(844..1404)
                     /locus_tag="VGB85_25400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137455.1"
                     /translation="MRTLLAVFVLLAACGPQSGEGTAAETGASAGMTDEGIGTSTGAS
                     TASTSGTTEDPTGASTTSGTSEDPTGAGQCELAIGPLEVVEDDPHGCLGFTDEASCLA
                     VYGHCTAIYGYPARCDTSWCRTASERVVIACRPLEICKEASIMVCRMTDAGIEAFWSE
                     RCVPPGFGRCEPGLPAGYGPPPACAP"
     gene            1535..2107
                     /locus_tag="VGB85_25405"
     CDS             1535..2107
                     /locus_tag="VGB85_25405"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0137456.1"
                     /translation="MKATPAELDELTLARAQRGDDRARRDLVDRYQRPVFALLSRMLF
                     QRGQQQALEDLAQETFLRVFKALPGFTRGGPARLSTWILTIATNLAIDHLRKRRVPTD
                     ALTDTQEPAANETSDAEAERRRLAEVLRRAIDDLSPEYRAAFLLREYHEFEYAEIAST
                     LEIDLGTVKSRLNRARAALRRSLEEVYRAR"
     gene            2097..>2484
                     /locus_tag="VGB85_25410"
     CDS             2097..>2484
                     /locus_tag="VGB85_25410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137457.1"
                     /translation="MHDEPRNELEQRALAAWTPAEPPADFVDRVMRAAATDSRADRVA
                     DVLDGVPLPRLRWPRFVAAAIAAVALAGGVTAGIKYWPAPVASAPTESRDSIDDGVPV
                     SLTPPAAQAPKDDVPADLDQQIDRYMA"
BASE COUNT          381 a          982 c          805 g          316 t
ORIGIN      
        1 catcatggtg gagctccgcg acgcagcccg tgggtggtcc gccagtcgtc gcgcgcgccg
       61 gcccggttca taagtccgag ctgcgccggc cacccaccga cgcgattcca cgacccgaac
      121 acccggtcga gcagcgtcag cttgcccgcc aggttgcgcc gctgatcctc gaccgcgacc
      181 gcgtggtgcc acgcgtgcac ccgcggcgtg acgagcaccg actcgagcca gccgagcctg
      241 atcgacgagt tgaggtgcgt gaacccggtc aacaccaccc cgaacagcgt gtgaaacaac
      301 atcacctcga ccgcgggccc gagcagcacc agcaccggca gcggcgcgag gccgtgcaac
      361 agcgcctcgc cggtgtgctt gcgaaagccc gcgagccagt cgatctcggc cggtgcgtgg
      421 tgcggcgcgt gcagccgcca cagccccggc cacgtgtgca tgccgcggtg caccgcataa
      481 aacgtcagct cggtgactgc caaaaccacc agccactgca cgaacatcgg cagggccggc
      541 agcgggccgg tgacatgcag cgccgcgccg agccgtccga ccgcccaccc gggcagcgcg
      601 ttcgacagca gcacgtacgg cagctcgagc gcgagcgccc cggcgagcgc acgggtcggg
      661 cgtgccggcc agcgccgctc gagccacatg aacagcagcc cgctcaccac gaaggtgacg
      721 accgagagca ccaggaagat cacgccgggc ccccgtggcc tggctgcatc acgcgtcggg
      781 ccggcatggt cggcagagta ccaaagtcgc cgcgcaaggc cgtccgtcgc accggtcgca
      841 ggctcacggc gcgcacgcgg gcggcggccc gtacccagcc ggcaggcccg gctcgcagcg
      901 accgaagccc ggcggcacac agcgctcgga ccagaacgct tcgatccccg cgtcggtcat
      961 gcgacagacc atgatgctcg cctccttgca gatctcgagc ggacgacagg cgatgaccac
     1021 ccgctcggac gcggtccgac accagctcgt gtcgcagcgc gccgggtagc cgtaaatggc
     1081 cgtgcaatgc ccgtacacgg ccaggcagct cgcctcgtcg gtgaacccga ggcacccgtg
     1141 cgggtcgtcc tccaccacct ccagcggccc gatcgccagc tcgcactggc ccgcgccggt
     1201 cgggtcctcg ctcgtcccgc tcgtggtcga cgccccggtc ggatcctcgg tcgtcccgct
     1261 cgtgctcgcg gtcgacgccc ccgtcgacgt cccgatcccc tcgtcggtca tcccggccga
     1321 cgcccccgtc tctgccgcgg tcccctcgcc gctctgcggt ccacaggccg caagcagcac
     1381 gaacacggcg agcagggtcc tcatcgacct cttctcgcac actttcgcgc gcgagtccgc
     1441 gcccctttgc gccgtcacgt ttctccggga acaaacgccg ggcgcgcccg tgccatgcta
     1501 ggtgaccgtc gccgcccccc gggcctgggc tcccgtgaaa gcaacaccag ccgaactgga
     1561 cgagctgacg ctggcgcgcg cgcagcgggg tgacgaccgg gcgcgccgcg acctcgtcga
     1621 ccgttaccag cggccggtgt tcgccctgct ctcgcggatg ttgtttcagc gcggtcagca
     1681 gcaggcgctc gaggacctcg cgcaggagac ttttttgagg gtgttcaagg ccctgcctgg
     1741 cttcacgcgc ggcggcccgg cgcggctctc gacgtggatc ttgacgatcg ccaccaacct
     1801 tgcgatcgac cacctgcgca agcgtcgggt cccgaccgac gccctgaccg acacccagga
     1861 gccggcggcc aacgagacca gcgacgccga ggccgagcgc cgccggctcg ccgaggtcct
     1921 gcgccgcgcg atcgacgacc tgtcccccga gtatcgcgcc gcctttctgc tccgcgagta
     1981 tcacgagttc gaatacgccg agatcgccag cacgctggag atcgacctcg gcaccgtcaa
     2041 gtcgcggctc aaccgcgcac gcgcggccct gcgtcgctcg ctggaggagg tgtaccgtgc
     2101 acgatgaacc ccgcaacgag ctcgagcagc gcgccctcgc agcctggacc ccggccgagc
     2161 cgccggccga cttcgtcgac cgcgtcatgc gagccgccgc gaccgacagc cgcgccgacc
     2221 gcgtcgccga cgtgctcgac ggcgtgccgc tgccacgact tcgctggccc cgcttcgtcg
     2281 ccgctgcgat cgcagcggtc gcgctcgcgg gcggcgtcac ggccggcatc aagtactggc
     2341 ccgcgcccgt cgccagcgcc ccgaccgagt ctcgggactc gatcgacgac ggcgtgccgg
     2401 tctcgctgac gcccccggcc gcgcaggccc ccaaggacga cgtcccggcc gacctcgacc
     2461 agcagatcga ccgctacatg gcga
//
LOCUS       DASWNJ010001529         2153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_63474, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001529 DASWNJ010000000
VERSION     DASWNJ010001529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63474"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1490)
                     /locus_tag="VGB85_25415"
     CDS             complement(<1..1490)
                     /locus_tag="VGB85_25415"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0137458.1"
                     /translation="MNTSHRSTRSSLGIAALCAPLLTLTACGVSGGFDEDAAGATAGG
                     TGVATTQTTASGGENGDEGGGEEGLGVGQGGSQDFGQFKEILDRGEIPGPNTIDDVGF
                     FNEHKLTLPAPACPNDVCVHGLFGQMGNMITGSDCITVLVGLNTAIDVDKLTRPPLNL
                     SLVIDTSGSMEGEPIAYVRDGLMRMLDGLEAGDHISLITFSTTAKVQVEAVTGDAPEL
                     INAIDAIAASGGTNIYDGLRTGFELVAEHYQEGAQNRVILLSDGNATEGITNSAKIVS
                     MAAGYSAQGFGLNTIGVGADFDVELMRTLSEQASGSFYFLEDPAAVQEVFHEEVTSFL
                     VPLAEEVSLDVDIGDAYVLRGIYGTKLFDFTNGAGSIDIPNLQLAHRTDVDDHENGGR
                     RGGGGAILLELLRKNGAAQTQDVGDMMLRYRVPKTDTFVEEKVAIQIPVAPDQVDLEN
                     GTFEDLSVEKGFVMLNIYVGFQMAATRASQGDFNGAWKVLDSLDKNV"
     gene            complement(1532..>2153)
                     /locus_tag="VGB85_25420"
     CDS             complement(1532..>2153)
                     /locus_tag="VGB85_25420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137459.1"
                     /translation="PVDAYYPSPDVERVAWSDPPDTGVAMQLTTLDVGPDGCLGLEFD
                     DALAVPRWYLCLPPDSFPFAQGDWLTVDSHEAGEVLELQHVAGPMDVDPPPAVAMVVA
                     RGRSLPKLANTVMAAKATYDVSIAPDPVCGTVARPSEISVRYEEAVPQTAGPGDMISL
                     QGADTTLALWVTHAEERVLLNPACAEGPDELGADLEIVAVRAPSAP"
BASE COUNT          294 a          686 c          810 g          363 t
ORIGIN      
        1 acgttcttgt cgaggctgtc gagcaccttc caggcgccgt tgaagtcgcc ctggctcgcg
       61 cgcgtcgcgg ccatctggaa gccgacgtag atgttgagca tcacgaagcc cttctcgacg
      121 ctgaggtcct cgaaggtgcc gttctcgagg tcgacctggt ccggggcgac ggggatctgg
      181 atcgcgacct tctcctcgac gaaggtgtcg gtcttgggga cgcggtagcg cagcatcatg
      241 tcgccgacgt cctgcgtctg cgcggcgccg ttcttgcgca gcagctcgag caggatcgct
      301 ccgccgccgc cgcgacgacc gccgttctcg tggtcgtcga cgtccgtgcg gtgggcgagc
      361 tggaggttgg ggatgtcgat gctgccggcg ccgttggtga agtcgaacag cttggtcccg
      421 tagatgccgc gcaggacgta ggcgtcgccg atgtcgacgt cgagcgagac ctcctcggcg
      481 agcggcacga ggaacgacgt cacctcctcg tggaagacct cctgcacggc ggcggggtcc
      541 tcgaggaagt agaagctgcc ggacgcctgc tccgagagcg tgcgcatcag ctcgacgtcg
      601 aagtcggcgc cgacgccgat cgtgttgagg ccgaagccct gggccgagta gccggcggcc
      661 atgctgacga tcttggcgct gttggtgatg ccctcggtcg cgttgccgtc gctcagcagg
      721 atgacgcggt tctgggcgcc ttcctggtag tgctcggcga ccagctcgaa gccggtgcgc
      781 aggccgtcgt agatgttggt gccgccgctc gccgcgatcg cgtcgatcgc gttgatcagc
      841 tccggggcgt cgccggtgac cgcctcgacc tggaccttgg cggtcgtcga gaatgtgatc
      901 agcgagatgt ggtcgcccgc ctcgaggccg tcgagcatgc gcatcaagcc gtcacgcacg
      961 taggcgatcg gctcgccctc catcgagccg ctggtgtcga tgacgaggct gaggttgagc
     1021 ggcgggcgcg tcagcttgtc gacgtcgatc gcggtgttga ggccgacgag cacggtgatg
     1081 cagtcggagc cggtgatcat gttgcccatc tgtccgaaca ggccgtgcac gcacacgtcg
     1141 ttggggcagg ccggcgccgg cagggtcagc ttgtgctcgt tgaagaagcc gacgtcgtcg
     1201 atcgtgttgg gcccggggat ctcgccgcgg tcgaggatct ccttgaactg gccgaagtcc
     1261 tgcgagccgc cctggccaac gccgaggccc tcctcgccgc caccctcgtc gccgttctca
     1321 ccgccgctcg cggtggtctg ggtggtcgcc acaccggtgc cgccggcggt ggcgccggcc
     1381 gcatcttcgt cgaagccgcc ggagacgccg caggcggtga gcgtcagcag cggggcacac
     1441 agcgcggcga tgccgaggga ggagcgcgtc gatcgatgag aggtgttcat ggcagggttc
     1501 ctgttaagaa atggcttcga cgggaagagg ttcacggggc gctgggcgca cgcacggcga
     1561 cgatctcgag gtcggcgccg agctcgtcgg ggccctcggc gcaggcgggg ttgagcagca
     1621 cgcgctcctc ggcgtgggtg acccacaggg cgagcgtggt gtcggcgcct tgcaagctga
     1681 tcatgtcgcc ggggccggcg gtctgcggga cggcctcctc gtagcggacg ctgatctcgc
     1741 tcggccgcgc gacggtgccg cagaccgggt ccggggcgat gctgacgtcg taggtcgcct
     1801 tggcggccat caccgtgttc gccagcttgg gcaggctgcg gccgcgggcg acgaccatcg
     1861 ccacggcggg cggcgggtcg acatccatcg ggccggcgac atgttgcagc tcgagcacct
     1921 cgcccgcctc gtgggaatcg acggtcagcc agtcgccctg cgcgaacggg aagctgtcgg
     1981 gtggcaggca caggtaccag cgcggcacgg cgagtgcgtc gtcgaactcg aggccgaggc
     2041 agccgtcggg gccgacgtcg agggtggtca gctgcatggc gacgccggtg tccggcgggt
     2101 cgctccaggc gacgcgctcg acgtccgggc tcgggtagta ggcgtcgacc ggc
//
LOCUS       DASWNJ010001530         2591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_449192, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001530 DASWNJ010000000
VERSION     DASWNJ010001530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449192"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1016
                     /locus_tag="VGB85_25425"
     CDS             63..1016
                     /locus_tag="VGB85_25425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137460.1"
                     /translation="MPRPRPAAPPAVAPGRRVPEGRGAAARALALVVAVTLAPGVARA
                     GEPPAAGDDLTEQTGDATSEVSQRTGPAEVDGDLSVQTAPGAEEATRWTAEGQRRRTA
                     GDLDGAIEAWQRATAVLPVTQATAHRRAGLALAVAAAHAEAARARPERMRRAIAVLDA
                     YLAGLDPTDDENRVAVEQRRAELAARLGPDPTVTPTTPAPAPPRRDRRLLVAGGVGLG
                     LGAAGLTVLAAGLLAGARADRELAAAVELPGDDPRREPDKDAAWARGLRANRAAIAGG
                     VLGGVLLVAGLALTIAGVVRRPPRRAALTPTLAPGGLRWAF"
     gene            complement(1045..1653)
                     /locus_tag="VGB85_25430"
     CDS             complement(1045..1653)
                     /locus_tag="VGB85_25430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0137461.1"
                     /translation="MDDEAMFAAWRAGDARAGAELFERHYAGIARFFRNKVDADAPDL
                     IQKTFLGCLESRERVRGDSSFRTYLFAVAHNVLYKHLRARSRAPIDFGLTSIHDLRPT
                     ATALLARRAEEQALLQALRRIPLEHQVTLELYFWDSFTAREIAEIQGVPEGTVRTRLR
                     RAKQLLTDALAEVAESPAVLHETVSNLDAWASSVRAQIAGRP"
     gene            complement(1839..>2591)
                     /locus_tag="VGB85_25435"
     CDS             complement(1839..>2591)
                     /locus_tag="VGB85_25435"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0137462.1"
                     /translation="AQIRRERSDRRAALADQVTDRLGGKVRVIVARDAELTAYREFLV
                     SLMKRSGKQYNAPIERLTAQVAPDKLGALCDREDHAALAASGDINPEFAESLIRQLRE
                     QPERRFELDVLDIEDVPRIELQVHGEWRSADRLSTGQKSSAILPILLMDSEAPLLIDQ
                     PEDNLDNSFISDAVIPQILASKGKRQLVLITHNPNLPVLGEAAQVVVLEADGRHSKVV
                     AQGDVEAVKQHIVRLMEGGREAFRQRARSYGA"
BASE COUNT          324 a          914 c         1000 g          353 t
ORIGIN      
        1 aaggtcacgg gcgtcggcgg gctgtcgctg cctccctgcc tggtctacca tgccggcggt
       61 cgatgccacg cccgcgcccc gccgctccgc ccgcagtcgc gccgggccgc cgggtccccg
      121 aaggccgggg ggccgccgcg cgggcgctcg cgctggtcgt cgcggtcacg ctggcgccag
      181 gagtcgcgcg cgccggcgag ccgccggccg cgggggatga cctgaccgaa cagacaggtg
      241 atgcgacatc ggaggtgtct caacggacag gtccggccga ggtcgatggt gacctgtccg
      301 ttcagacagc ccccggcgcc gaggaggcga cgcgctggac cgcggagggc cagcgccggc
      361 ggacggcggg cgacctcgac ggcgcgatcg aggcctggca gcgggcgacg gcggtcttgc
      421 cggtgacgca ggcgacggcg caccggcggg ccggcctcgc gctggcggtc gcggcggccc
      481 acgcggaggc ggcccgggcc cggcccgagc ggatgcgacg ggcgatcgcc gtgctcgacg
      541 cgtacctcgc cggcctcgac ccgaccgacg acgagaaccg cgtcgccgtc gagcagcgcc
      601 gcgccgagct ggccgcgcgc ctcgggcccg acccgaccgt cacgccgacg acgcccgcac
      661 cggcgccccc gcgccgcgac cgtcgactgc tcgtcgccgg tggggtcggg ctcggcctcg
      721 gcgcggccgg cctgacggtg ctggctgccg ggctgctcgc gggtgcgcgg gccgaccgcg
      781 agctggcggc tgccgtcgag ctgccaggcg acgacccgcg ccgcgagccc gacaaggacg
      841 ccgcgtgggc ccgcggtctg cgggccaacc gcgccgcgat cgcaggcggc gtgctcgggg
      901 gggtgctgct cgtcgccggt ctcgcgctga cgatcgccgg ggtcgtgcgc cggccgccac
      961 gccgcgccgc gctcactccg acgctcgctc cgggcggcct gcgctgggcc ttttaaaaaa
     1021 agaggacatg tcccgtggga caggtcaggg gcggccggcg atctgggcgc gcaccgagct
     1081 ggcccacgcg tcgaggttgc tgacggtctc gtgcagcacg gccggtgact cggcgacctc
     1141 ggcgagcgcg tcggtcagca gctgcttggc gcggcgcagc cgggtgcgca cggtgccctc
     1201 cggtacgccc tggatctcgg cgatctcgcg ggcggtgaag ctgtcccaga agtacagctc
     1261 gagggtcacc tggtgctcga gcgggatccg tcgcagggcc tgcagcagcg cctgctcctc
     1321 ggcgcgacgg gccagcaacg cggtcgcggt cggccgcagg tcgtggatcg aggtgaggcc
     1381 gaagtcgatc ggtgcgcgtg aacgggcccg caggtgcttg tagagcacgt tgtgcgcgac
     1441 cgcgaacaga taggtgcgaa agctcgagtc accgcgcacc cgctcgcgcg actcgaggca
     1501 gccgaggaag gtcttttgga tcaggtcggg cgcgtcggcg tcgaccttgt tgcgaaagaa
     1561 ccgggcgatg ccggcgtagt ggcgctcgaa cagctccgcg ccggctcgcg cgtcgccggc
     1621 tcgccacgcg gcaaacatcg cctcgtcgtc catcgccgga ccagcataca ccaggcggag
     1681 cgtgcgcgcg gcgtgcgcct gggtgacgcc gggagacgat ggcgagatcc aaggatgaag
     1741 cggtccgcac cgccgcgagg cgcaacttca accaggcgcg aagacctctg tcacggaccg
     1801 ccagtcgctc gccagcgagc tggcgtggcg gggtgctttc aggcgccgta gctgcgggcg
     1861 cgctggcgga aggcctcgcg tccgccctcc atgagccgca cgatgtgctg cttgaccgcc
     1921 tcgacgtcgc cctgggcgac gaccttgctg tggcggccgt ccgcctcgag cacgaccacc
     1981 tgcgcggcct cgccgaggac cgggaggttg gggttgtgcg tgatgaggac cagctgccgc
     2041 ttgcccttgc tggcgaggat ctgcgggatg acggcgtccg agatgaagct gttatccaaa
     2101 ttgtcctccg gctggtcgat caacagcggt gcctcgctgt ccatcaggag tatcggcaag
     2161 atcgccgagc tcttctggcc ggtcgacagg cggtcggcgc tgcgccactc gccgtggacc
     2221 tggagctcga tgcgcgggac gtcctcgatg tcgagcacgt cgagctcgaa gcgccgctcg
     2281 ggctgctcgc gtagctggcg gatcaggctc tcggcgaact cggggttgat gtcgccgctg
     2341 gcggccagcg cggcgtggtc ctcgcggtcg cacagcgcgc cgagcttgtc cggcgcgacc
     2401 tgggcggtca ggcgctcgat cggcgcgttg tactgcttgc ccgagcgctt catcaggctg
     2461 accaggaact cgcggtacgc ggtcagctcg gcgtcgcggg cgacgatgac gcggaccttg
     2521 ccgccgaggc ggtcggtcac ctggtcggcg agcgcggcgc gccggtccga gcgctcgcgg
     2581 cggatctgcg c
//
LOCUS       DASWNJ010001531         2986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_204754, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001531 DASWNJ010000000
VERSION     DASWNJ010001531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204754"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="VGB85_25440"
     CDS             complement(<1..1067)
                     /locus_tag="VGB85_25440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0137463.1"
                     /translation="MTTTTRSPRTRSLWSKWPATTISALTGLTALALVLATGRVEIDA
                     QAPLEASTEGMAMPVAFEVAAPDVGFKIAAPESESAEKREMAARSAGILGVMAEEKGH
                     FLASPYGGAFAVGSDDADVWGGLAGTEVGEAYGVGGLGLVGTGRGGGGTGEGTIGLGS
                     GGSLGKGGSGSGYGRGAGSERRSRGSYNASLQSHVLTVGTVDDNADLAGYRKALRKLK
                     EEGGMALGIDESLWQMAAPKQRHAAAPGGLDVALVIDTTGSMGDELEYLKVELRDIAR
                     EVSTSFPGVTQRWGLVVYRDRGDAYVTRSVDFRGIDEFITKLGEQEAGGGGDHPEAMD
                     LAMDASAELSWRTGEGTARMV"
     gene            1249..2283
                     /locus_tag="VGB85_25445"
     CDS             1249..2283
                     /locus_tag="VGB85_25445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137464.1"
                     /translation="MTTSCQQCGAGIPDGRGTSRCLACRRGLTGARDEHSVLFSLADF
                     AAAGPALPRSAPASQRSRSDEGSGLIDIRAMQAALGGRAEPAPPRFITPTALPVAPPP
                     TASHRAVPSQLPLYGLLAALMFGLASLTAFVLSEPAAAPPPTPRHTIPAPVVLAAPVP
                     EEVEEPEEIDEPVAEPVAVEPVAKVTTSRPRPSQRPTPAVARPQPEVVKPQAASKPAS
                     SDEDTMKCLLGQGACKVERPAEAPRPAVAAPVASDLPERLSDTDITAGTGPAKARATS
                     SCARLAKPGERVKIKLSIAGPTGTVIRATAEDDGGNPALAACCAGELAKASFKPVQRP
                     QMGALVTLKF"
     gene            complement(2293..>2986)
                     /locus_tag="VGB85_25450"
     CDS             complement(2293..>2986)
                     /locus_tag="VGB85_25450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137465.1"
                     /translation="GAPGGVIVALVDGATGKVDEQITIPTFSGDSFGAYGGAVNAQGD
                     LFFTSMAINGGRLARVYIKNFAYKVWDIPPEIGPYGITVDHTGRVWISSVAINSNGAG
                     RFDPETEQWDFVSGFGTGAGLAEGPDDWMWVSSFNGVNAVHIDTLELGPVFKTDYTIK
                     GVGFDAKGFLWAVNYAETDEGNQPIEPELVMKVDTATLAVVGQYEGLDRPYTYSDFTG
                     NALFNVSCVPPQ"
BASE COUNT          410 a         1177 c          982 g          417 t
ORIGIN      
        1 aacaccatgc gcgccgtccc ctcgccggtg cgccacgaca gctcggcgct cgcgtccatc
       61 gccaggtcca tcgcctcggg atggtcgccg ccgccgccgg cctcctgctc gccgagcttg
      121 gtgatgaact cgtcgatccc acgaaagtcg acgctgcggg tcacgtacgc gtcgccgcgg
      181 tcgcggtaga ccaccaggcc ccagcgctgc gtcacgcccg ggaagctcgt cgacacctcg
      241 cgcgcgatgt cccgcagctc caccttcaag tactcgagct cgtcgcccat gctgccggtc
      301 gtgtcgatca ccagcgcgac atcgagcccg cccggcgccg ccgcgtggcg ctgcttcggc
      361 gccgccatct gccacagcga ctcgtcgatg ccgagcgcca tgccaccctc ctccttcagc
      421 ttgcgcagcg ccttgcgata cccggcgagg tcggcgttgt cgtcgacggt gccgaccgtc
      481 aatacgtgcg actgcaggct cgcgttgtag ctgccgcggc tgcgccgctc gctgccggcg
      541 ccgcgcccgt agcctgaacc gctgccgccc ttgccgagcg acccgccact gccgagcccg
      601 atcgtcccct cgccggtgcc gccgccgccg cgcccggtgc cgaccaggcc gagcccgccg
      661 accccgtagg cctcgccgac ctcggtgccg gcgagaccac cccacacgtc ggcgtcgtcg
      721 gagccgaccg cgaacgcgcc gccatagggc gacgccagga agtgaccctt ctcctccgcc
      781 atcacgccga ggatgccggc cgatcgcgcc gccatctcac gcttctcggc cgactccgac
      841 tccggcgcgg cgatcttgaa cccgacgtcc ggcgccgcca cctcgaacgc gaccggcatc
      901 gccatcccct ccgtgctcgc ctcgagcggt gcctgtgcat cgatctcgac ccggccggtc
      961 gccaggacca gggcgagcgc ggtcaggccg gtgagcgcgg agatcgtcgt cgcgggccac
     1021 ttgctccaca ggctgcgcgt gcggggggaa cgggtcgtcg tcgtcatggc gggcaagaca
     1081 gcgggccccc ggcgaaagtt cccggtcgat cttgcgactc gtatagatcg ccggcgagct
     1141 tgcggcgttg agaccccaca cttgcagcca ggcgtgcacc agcatcccca cggagctccg
     1201 tggtctgccg gtgcgcaccg gtgggtctgc gttcaaggag cgacgatcgt gacgacttca
     1261 tgccagcagt gcggcgccgg gatcccggac ggccgtggaa ccagccgttg tcttgcctgc
     1321 cgtcgcggcc tcaccggtgc gcgcgacgag cactccgtcc tcttctccct cgccgacttc
     1381 gccgccgccg ggcccgcgct gccgcgcagc gcaccggcgt cgcagcgctc gcgcagcgac
     1441 gagggctccg gcctgatcga catccgcgcg atgcaggccg ccctcggcgg gcgggccgag
     1501 cccgcgccgc cgcgcttcat cacccccacg gcgctgcccg tcgcaccccc gccgacggcg
     1561 agccaccgcg ccgtgccctc gcagctgccc ctctacggcc tgctcgccgc gctgatgttc
     1621 ggcctcgcca gcctgaccgc cttcgtgctc agcgagccgg cggccgcacc tccgccgacg
     1681 ccgcgccaca cgatccccgc gccggtcgtg ctcgccgcgc ctgtgcccga ggaagtggag
     1741 gagccggagg agatcgacga gcccgtcgcg gagccggtcg cggtcgagcc ggtcgccaag
     1801 gtcaccacct cacgaccgag gccctcgcag cgcccgacgc cggcggtcgc caggccgcag
     1861 cccgaagtgg tgaagccgca agcggcgagc aagccggcca gcagcgacga ggacaccatg
     1921 aagtgcctgc tcggccaggg cgcgtgcaag gtcgagcgcc ccgccgaggc gccgaggccc
     1981 gccgtcgccg cgccggtcgc ctccgatctg cccgagcgcc tcagcgacac cgacatcacc
     2041 gcgggcaccg gcccggccaa ggcccgggcg acgagcagct gcgcgcgtct ggcgaagccc
     2101 ggcgagcgcg tgaagatcaa gctctcgatc gccggcccga ccggcaccgt catccgcgcg
     2161 accgccgagg acgacggcgg caacccggcg ctggcggcct gctgcgcggg tgagctcgcc
     2221 aaggccagct tcaagccggt ccagcgcccg cagatgggcg ccctcgtgac gctcaagttc
     2281 taggcgcgcg gctcactgcg gcggcacgca gctgacgttg aacagcgcgt tgccggtgaa
     2341 gtccgaatac gtgtagggcc gatcgaggcc ctcgtactgc ccgaccaccg cgagcgtcgc
     2401 ggtgtcgacc ttcatcacca gctcgggctc gatcggctgg ttaccctcgt cggtctcggc
     2461 gtagttgacg gcccacagga agcccttggc gtcgaagccg acccccttga tcgtgtagtc
     2521 ggtcttgaac accgggccga gctcgagcgt gtcgatgtgc accgcgttga cgccgttgaa
     2581 cgacgacacc cacatccagt cgtcgggtcc ctcggcgagg cccgctccgg tgccgaagcc
     2641 gctgacgaag tcccactgct cggtctcggg gtcgaagcgt ccggccccgt tgctgttgat
     2701 cgccaccgac gagatccaca cgcgcccggt gtggtcgacc gtgatgccgt agggcccgat
     2761 ctccggcggg atgtcccaca ccttgtaggc gaagttcttg atgtacacgc gcgcgagccg
     2821 gccgccgttg atcgccatcg acgtgaagaa caggtcgccc tgcgcgttga cggccccgcc
     2881 gtaggccccg aacgagtcgc ccgagaacgt cgggatcgtg atctgctcgt cgaccttgcc
     2941 ggtcgcgcca tcgaccagcg ccacgatcac cccgcccggc gcgccg
//
LOCUS       DASWNJ010001532         1544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_166852, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001532 DASWNJ010000000
VERSION     DASWNJ010001532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166852"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VGB85_25455"
     CDS             complement(<1..293)
                     /locus_tag="VGB85_25455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137466.1"
                     /translation="MREHRLSYNQNAQTIRTIAVSELGFAARPSAATLQIVDRRYSDT
                     DDNYSVVDAAVAVVDTVDTVTTAACGRSSADPATINVSSAVGIVAGRRYLITN"
     gene            complement(301..543)
                     /locus_tag="VGB85_25460"
     CDS             complement(301..543)
                     /locus_tag="VGB85_25460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137467.1"
                     /translation="MLTWRDVYLEEVERQVGLLRDAKARGQTWIVEDGRTYDPTRMSD
                     QQVRNLALRTAERTTNYAINLELEQARKKGRPADLN"
     gene            complement(546..1028)
                     /locus_tag="VGB85_25465"
     CDS             complement(546..1028)
                     /locus_tag="VGB85_25465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137468.1"
                     /translation="MGINTVEKWVVSSDPGERKLWIVPVVNPQGSIKYVRVSPHVQSG
                     IVAGIYDERAMDPRLATVQLKPGLSESGWAFLADLYAEDGLGAAWEAHRQWMRSGPMM
                     GAASAVKPFPEKYLPSGVHDRRAGKADHQVEADEIVIPEMDNRPVHTSKAKKRAAGMD
                     "
     gene            complement(1087..>1544)
                     /locus_tag="VGB85_25470"
     CDS             complement(1087..>1544)
                     /locus_tag="VGB85_25470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137469.1"
                     /translation="HNGLALDKAKFEAALLAFKKQRPNPGPQIFVGGYKQVADVIGAY
                     ADAGGSVYAVPGLVSGAVGGTQDASIYKRGVVAGIELYEAAVPASGGADVSGAFMVKS
                     RALALGFWQMLDFKLTDPAGRIGVELMTFSRYGTGIASQANLREVISLA"
BASE COUNT          248 a          504 c          513 g          279 t
ORIGIN      
        1 ctatttgtga tcaagtagcg tcgaccagcg acgatgccca ccgcgctcga cacgttgatc
       61 gtcgcggggt ctgcactcga ccgaccgcac gctgcggtgg tcacggtgtc gacggtgtcg
      121 acgaccgcga ccgcggcatc gacgacggag tagttgtcgt ccgtatccga gtagcgcctg
      181 tcgacgatct gaagtgtggc cgcagacggg cgcgcagcga agccgagttc cgacacggcg
      241 atcgtgcgga tggtctgggc gttttggtta tagctcagcc ggtgctcacg catgcgtccc
      301 tcagttcagg tcggccgggc ggcccttctt gcgggcctgc tcgagctcga ggttgatcgc
      361 gtagttcgtg gtgcgctccg cggtgcgaag cgcgaggttg cggacctgct ggtccgacat
      421 gcgagtcggg tcgtaggtgc gaccgtcctc gacaatccag gtctgcccgc gggctttcgc
      481 atcgcggagc aggccgacct gccgctcgac ctcctcgagg taaacgtcgc gccaggtcag
      541 catggtcagt ccatccccgc ggcgcgcttc ttggccttcg aggtatggac cgggcgattg
      601 tccatctcgg ggatgacgat ctcgtccgcc tcgacttggt gatcggcctt acctgcgcgg
      661 cggtcgtgga ccccactggg caggtacttc tccgggaacg gcttgaccgc tgaggcagcg
      721 cccatcatgg gacccgagcg catccactgg cggtgtgctt cccacgcggc gccgagtccg
      781 tcttcggcgt agaggtccgc gaggaacgcc cagccactct ccgagagacc cggcttgagc
      841 tggacggtcg ccagccgcgg gtccatcgcg cgctcgtcat agatgcctgc gacgatgccc
      901 gactgcacgt gcgggctgac acgaacgtac ttgatcgaac cctgcgggtt gacgaccggc
      961 acaatccaga gcttgcgctc acccgggtcg ctggagacga cccacttctc gacggtattg
     1021 attcccatga tggactactc gaggccggag tctgctcaat gcgagacccc ggccggttca
     1081 ctcagatcag gcgaggctga tgacctcgcg gaggttggcc tggctggcga tgcccgtgcc
     1141 gtagcggctg aaggtcatca gctcgacgcc gatgcgacct gcggggtcgg tcagcttgaa
     1201 gtccagcatc tgccagaagc cgagcgccaa tgcgcggctc ttgaccatga aggcgcccga
     1261 cacgtcagca ccacccgacg cggggacagc cgcctcgtac agttcgatgc cagcgacgac
     1321 accgcgctta tagatgctcg catcctgcgt gccgccgaca gcaccggaga ccaggcccgg
     1381 gaccgcgtag acggagccac ccgcatcagc gtaggcaccg atcacatcgg cgacctgctt
     1441 gtagccgccg acgaagatct gcgggccggg gttcggccgc tgcttcttga acgcgagtag
     1501 ggcagcctcg aacttcgcct tgtcgagcgc gaggccgttg tggt
//
LOCUS       DASWNJ010001533         2450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_558961, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001533 DASWNJ010000000
VERSION     DASWNJ010001533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558961"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1264)
                     /locus_tag="VGB85_25475"
     CDS             complement(50..1264)
                     /locus_tag="VGB85_25475"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEY0137470.1"
                     /translation="MNGQDLAAGVGWNGDPDSFAAGATAGRAALEQARSHAPVDPVAA
                     IVYSTVLYDQEAVLRGVRSVLPGARIVGASTQDISRTGDVESGDRVVGVAVLGGSGIT
                     ARSAYAPNVSKDPANAARMLAEQLGPPPEPDGPPLLLWYDPLTGINVDTLIAGLRAAG
                     YTRILGGGAGQPWGPVYRTYQYFEGRVTRDSAVVLRLGGAVDFVHDLTHGTDTLGLEL
                     EVTAAEGNVISAIDGRPALDVWREQLGLGDEDILDIEATAAWALGVSVTMEPGHDYEG
                     PITRSVFGFRTKTREVVLQAPIPAGTQVQVCHRTPEAVFDRAAVMATRLHTRLAGRSP
                     VLALSFECAARARPFLGEELTRREIGRMQAILGEDLPWLGMYAWGEVAPVGPSTYFHN
                     YTFPLAILCRRG"
     gene            1397..2023
                     /gene="nth"
                     /locus_tag="VGB85_25480"
     CDS             1397..2023
                     /gene="nth"
                     /locus_tag="VGB85_25480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEY0137471.1"
                     /translation="MTVPAELLLARLRDIHPSARYELDWSTPVQMLVATILAAQCTDE
                     RVNAVTPALFARWPDAAALAEADLGELEEAVRPTGFYKQKARSVQATCKALVERFGGE
                     VPQTMAELVTLPGVARKTANVVLATCFDIPEGIIVDTHVARVSQRMGLSGQRDPDRIE
                     QDLMPQIPEDQWTWFGPAMVLFGRYLCTAKKPACDRCPVATDCPKIGV"
     gene            complement(2026..>2450)
                     /locus_tag="VGB85_25485"
     CDS             complement(2026..>2450)
                     /locus_tag="VGB85_25485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137472.1"
                     /translation="GELARHELLEFHVAAAPARDPGPSRPFTLDARLTLDASVTLVRY
                     SHAVHLLPDDEDDRSEPAPTPTVLLAYRDAAYRVRYLQLTPLAEAVVAALLAGATVHA
                     AVLAGAAAESAALDDERLARLGALLADLAERRVILGAA"
BASE COUNT          337 a          859 c          931 g          323 t
ORIGIN      
        1 ggcgaggcga cggagggagc tgatcatttt gaaccgccag atcgcccggc taaccgcgtc
       61 tgcacaggat ggcgagcggg aaggtgtagt tgtggaagta ggtcgacggg ccgaccgggg
      121 cgacttcgcc ccaggcgtac atgccgagcc agggcaggtc ctccccgagg atcgcctgca
      181 tgcggccgat ctcgcggcgc gtgagctcct cgccgaggaa gggccgcgcc cgcgcggcgc
      241 actcgaagct gagcgcgagc acgggcgacc gtccggccag gcgcgtgtgc aggcgggtcg
      301 ccatcaccgc ggcgcggtcg aacaccgcct cgggggtgcg atgacacacc tgcacctgcg
      361 tacccgcggg gatcggcgcc tgcagcacga cctcgcgggt cttcgtgcga aagccgaaca
      421 ccgaccgcgt gatcggcccc tcgtagtcgt gaccgggctc catcgtcacg ctgaccccga
      481 gcgcccacgc ggccgtcgcc tcgatgtcga ggatgtcctc gtcgccgagg ccgagctgct
      541 cgcgccacac gtcgagcgcg gggcggccgt cgatcgcgct gatcacgttg ccctcggccg
      601 cggtgacctc gagctcgagc cccagcgtgt ctgtcccatg ggtcaggtcg tggacaaagt
      661 cgacggcgcc cccgaggcgc agcaccacgg cgctgtcgcg cgtcacgcgg ccctcgaagt
      721 actggtaggt gcggtacacc gggccccacg gctggccggc gccgccgccg aggatcctgg
      781 tgtagccggc cgcgcgcagc ccggcgatca gcgtgtcgac gttgatcccg gtcagcgggt
      841 cgtaccacag cagcagcggc gggccgtcgg gctcgggcgg cggtccgagc tgctcggcga
      901 gcatgcgcgc cgcgttggcg gggtccttgc tgacgttggg ggcgtacgcg gaccgggcgg
      961 tgatcccgga gccgccgagg accgcgacgc cgacgacccg atcgccggac tcgacgtcgc
     1021 cggtgcgcga gatgtcctgc gtcgaggcgc cgacgatgcg cgcgccgggc agcacgctgc
     1081 gcacgccgcg gagcaccgcc tcctgatcgt agagcaccgt cgagtagacg atggcggcca
     1141 ccgggtcgac gggggcgtgg cttcgggcct gttccagggc cgcgcggccg gccgtggccc
     1201 cggccgcgaa gctgtccgga tccccgttcc agcccacgcc tgccgcgaga tcctggccgt
     1261 tcatcgcggt catcgtcgca agccggcccc ggtctggcaa gtccacgaag gtaccgcgga
     1321 tcgtattccg gtcgtgcgcg cctgcgggcg tggctgccag cgcagaggcc gcgggagacg
     1381 tgatagcgtg tcgcgcgtga cggtcccggc cgagctcctc ctggcgcgcc tgcgcgacat
     1441 ccacccgtcg gctcgctacg agctcgactg gtcgaccccg gtgcagatgc tggtggcgac
     1501 gatcctcgcg gcgcagtgca ccgatgagcg cgtcaacgcg gtcacgccgg cgctgttcgc
     1561 ccgctggccg gacgcggcgg ccctggccga ggccgacctc ggcgagctcg aggaggcggt
     1621 gcgcccgacc ggcttttata aacaaaaggc gcgctcggtg caggcgacct gcaaggcgct
     1681 cgtcgagcgc ttcgggggcg aggtaccaca gacgatggcc gagctggtga cgctgcccgg
     1741 cgtcgcgcgc aagaccgcga acgtggtgct cgcgacctgc ttcgacatcc ccgaggggat
     1801 catcgtcgac acccacgtcg cgcgcgtcag ccagcgcatg ggcctcagtg gtcagcgcga
     1861 tccggatcgc atcgagcagg acctgatgcc gcagatcccc gaggaccagt ggacgtggtt
     1921 cggcccggcg atggtgctgt tcgggcgcta cctgtgcacc gcgaagaagc ccgcgtgcga
     1981 ccgctgcccg gtggcgacgg actgcccgaa gatcggcgtc tgagatcagg ccgcgccgag
     2041 gatcacgcgg cgctcggcga ggtcggctag caaggcgccg agtcgggcca ggcgctcgtc
     2101 gtccagcgcg gccgactcgg cggccgcacc cgcgagcacc gcggcgtgca cggtggcgcc
     2161 ggcgagcagc gccgcgacca cggcctcggc gagcggcgtc agctgcaagt aacgcacgcg
     2221 gtatgcggcg tcgcggtagg cgagcagcac ggtgggcgtc ggcgcgggct cgctgcggtc
     2281 gtcctcgtcg tcggggagca ggtgcaccgc gtgcgagtag cggaccagcg tgacgctggc
     2341 gtcgagcgtc aggcgggcgt cgagcgtgaa cggccgcgac gggcccgggt cgcgcgcggg
     2401 cgcggcggcg acgtggaact cgagcagctc gtgccgcgcg agctcgccga
//
LOCUS       DASWNJ010001534         3929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_696943, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001534 DASWNJ010000000
VERSION     DASWNJ010001534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696943"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1686
                     /gene="tssF"
                     /locus_tag="VGB85_25490"
     CDS             <1..1686
                     /gene="tssF"
                     /locus_tag="VGB85_25490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HEY0137473.1"
                     /translation="LQHFRGAARELGERHPGLAGQRGQPSDDPDLQRLIEAHAFLTAG
                     VHARLDAAAPAFVEALGDLLLPHVLRGVPSATIVELTPNLASLRARQRIARHRPLLAR
                     TIDGTACRFRTCFDLDLLPLELTTARLDDRIATRPQIRLTLRTRDACRGIFGELDRLR
                     LYVHHAAPAQAATLMQWFCRHCTGLALLDGEAEHARLPATAIDPIGLRGDPPIYPWPD
                     TAPPAHRLLLERFTLPERAHFLDIVGLHAVAPQATELTLVFSFDRPPPLPAQLDPGTF
                     RLHCTPAVNLFDTTAAAIHHTPLTREHLLRPDDLDPRHTEVHAVTRVLGHERARGTRR
                     CYGPLHDLSPEPGAPRYALRRAAAVDGGVDTFLLPREAPGAAHFPDELLSVDLVCSNR
                     DLPLQLGAGDLTGAPPGALFRSYRNLTPVTPPIRAPLGDDAQWHLLAHLGLAARGLHD
                     PEALRVLLGLHNAPARLRTPLGRANARQIDAIRTIERSLATRVHRGAATRTIRTRVEL
                     DEAAFASPGHAFLFSALLDRLFAGAAPFLTASELHTVLQPSAAELSWPPHLPT"
     gene            1733..2728
                     /locus_tag="VGB85_25495"
     CDS             1733..2728
                     /locus_tag="VGB85_25495"
                     /inference="COORDINATES: protein motif:HMM:NF018675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HEY0137474.1"
                     /translation="MSSSSPAPWLAELRAHPARHDLLAAYSLLRSLSPAPRNDLSEST
                     DDLPPDPVRFSHSADMALPIGELLAIEIDPDDPTRHLVTTAGPGPLGPGSPLPLALAD
                     DLDNPLAHAVLDLFHHRRTLLLCRGLLAADLPGTLDGEDPWSRRILALVGLADRTRTP
                     LTALRLAPIFAVPDRSPRALALALARLLPDLFTAPFTLRCEPISDRWTALPCDQHSRL
                     GRTSARLGDTAALGVAVRLPGTAARLTLGPLPASALDVLRPGGPAHTQLCALLADFIA
                     EPLDLELILELEDMSLPPGRLGQRTLGRDLWLTPDPAIRRPRHVALPLRPATTHG"
     gene            2760..>3929
                     /locus_tag="VGB85_25500"
     CDS             2760..>3929
                     /locus_tag="VGB85_25500"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0137475.1"
                     /translation="MPIPRLEIRVRDRRSGAATDHSFMHGPVRIGRDPACELPLADPY
                     VSNQHALLATDERGARLVDLGGTNGLRVAGRRLAPRTEIPVGERLCVSLGPFDLEVVH
                     TPDRDRQAVSGAVVEPGPVELERLHASLRRLHALHAPHVAARQAFEAALADTIHTFHG
                     TGDQPAIRRILAEFPTADHAHLILPTPPRAAPQPPASPRPPASVARREPQPLGPPNLS
                     LRTPSSAPGPHDLSPRTPSSTPDSLDAHDLSLRTPSCAPHSLDTPAHRPDAHDLSLRT
                     PPLDPSPANARPPAPHQTIPAATAPAPALALIADAAQTLLPDRRPPANLDEARRFFDR
                     LISTLRSLAAGVGALQHLRLQQARDLDLSLLDLANPLLTRTSADELLADLLAWRED"
BASE COUNT          468 a         1948 c          985 g          528 t
ORIGIN      
        1 ctccagcact tccgcggcgc cgcccgcgag ctcggcgagc gccaccccgg cctcgccggc
       61 cagcgcggcc aacccagcga cgaccccgac ctccagcgcc tgatcgaggc ccacgccttc
      121 ctcaccgccg gtgtacacgc ccgcctcgac gccgccgccc cggccttcgt cgaggctctc
      181 ggcgacctcc tgctccccca cgtcctgcgc ggggtcccga gcgcgacgat cgtcgagctc
      241 acccccaacc tcgcctccct gcgcgcgcgc cagcggatcg cccggcatcg cccgctgctc
      301 gcccgcacga tcgacggcac cgcctgtcgc tttcgcacct gcttcgacct cgatctgctc
      361 cccctcgagc tcaccacggc ccgcctcgac gaccgcatcg ccacccgccc gcagatccgc
      421 ctgaccctgc gcacccgcga cgcctgccgc ggcatcttcg gcgagctcga ccgcctgcgc
      481 ctctacgtcc accacgccgc cccggcccag gccgccaccc ttatgcagtg gttctgtcgt
      541 cactgcaccg gcctcgccct cctcgacggc gaggccgagc acgcccggct gcctgccacc
      601 gcgatcgacc cgatcggcct gcgcggcgac ccgccgatct acccctggcc cgacaccgcc
      661 ccgcccgcgc accgcctcct gctcgagcgc ttcaccctcc ccgagcgcgc ccacttcctc
      721 gacatcgtcg gcctgcacgc cgtcgccccg caggccaccg agctcaccct cgtgttctcc
      781 ttcgaccgcc cgccgccctt gcccgcgcag ctcgaccccg gcacgtttcg cctgcactgc
      841 acccccgccg tcaacctctt cgacaccacc gccgccgcga tccaccacac cccgctgacg
      901 cgcgagcacc tgctgcgccc cgacgacctc gaccctcgcc acaccgaggt ccacgccgtc
      961 acccgcgtcc tcggccacga gcgcgcccgc ggcacccgtc gctgctacgg cccgctgcac
     1021 gacctgtccc cggagccagg tgccccgcgc tacgccctgc gccgcgccgc ggccgtcgac
     1081 ggcggcgtcg acaccttctt gctcccccgc gaggcccccg gcgccgctca tttccccgac
     1141 gagctgctca gcgtcgacct cgtgtgcagc aaccgcgact tgccgctgca gctcggcgcc
     1201 ggtgacctca ctggcgcccc gcccggggcc ctgttccgca gctatcggaa cctcaccccc
     1261 gtcaccccac cgatccgcgc cccgctcggt gacgatgccc agtggcacct gctcgcccac
     1321 ctcggcctcg ccgcccgcgg ccttcacgac cccgaagccc tgcgcgtcct gctcggcctg
     1381 cacaacgccc ccgcccgcct gcgcaccccc ctcggccgcg ccaacgcccg ccagatcgac
     1441 gccatccgga ccatcgagcg cagcctcgcc acccgcgtcc accgcggcgc cgccacgcga
     1501 acgatccgca cgcgcgtcga gctcgacgag gccgccttcg ccagccccgg ccacgctttt
     1561 ctgttcagcg ccctgctcga ccgactgttc gccggcgccg cccccttcct caccgccagc
     1621 gagctccaca ccgtcctgca accctccgcc gccgagctct cctggccccc gcacctcccc
     1681 acctgaaaat ctccgcgcaa cactcgagcc ggcggcgccg agtccatggc ccgtgtcctc
     1741 gtcctcaccc gccccctggc tcgccgagct ccgcgcccac ccggcccgtc acgacctgct
     1801 cgccgcctac agcctgctgc gcagcctctc ccccgctccc cgcaacgacc tgtccgaaag
     1861 cacagatgat ctccccccgg acccggtccg cttcagccac agcgccgaca tggcgctccc
     1921 catcggcgag ctgctcgcga tcgagatcga cccggacgac ccgacgcgcc acctcgtcac
     1981 caccgccggc cctggcccgc tcggtcctgg ctccccgctc ccgctcgccc tcgccgacga
     2041 cctcgacaac ccgctcgccc acgccgtcct cgatctgttt caccaccgcc gcaccctgct
     2101 cctgtgccgc ggcctgctcg ccgccgatct gcccggaacc ctcgacggcg aggacccgtg
     2161 gtcgcgcagg atcctcgcgc tcgtcggcct cgccgatcgc acccgcaccc cgctgaccgc
     2221 attacgcctc gcaccgatct tcgctgtccc ggaccgctcg ccacgcgccc tcgccctcgc
     2281 cctcgcccgc ctgctccccg acctcttcac cgcccctttc acgctgcgct gcgagccgat
     2341 ctccgaccgc tggaccgccc tcccctgcga ccagcacagc cgcctcggcc gcacctccgc
     2401 ccgcctcggt gacaccgccg cgctcggcgt cgccgtccgc ctcccgggca ccgccgcgcg
     2461 actcaccctc ggtcctctgc ccgcctccgc cctcgacgtc cttcgccccg gcggccccgc
     2521 gcacacgcag ctgtgcgccc tgctggccga cttcatcgcc gagcctctcg acctcgagct
     2581 gatcctcgag ctcgaggaca tgtcccttcc gccaggtcgc ctcggccagc gcaccctcgg
     2641 ccgcgacctc tggctgaccc cggacccggc cattcgccgt ccccgacacg tcgccctccc
     2701 gctccgcccg gccacgaccc acggctgacc tgccctttaa cccacccgga atcacaacca
     2761 tgcccatccc tcgcctggag atccgcgtcc gagaccgccg cagcggcgcc gccaccgatc
     2821 acagcttcat gcacggcccg gtccggatcg gccgcgaccc cgcctgcgag ctccctctcg
     2881 ccgatcctta cgtctccaac cagcacgccc tgctcgcgac cgacgagcgc ggcgcgcgtc
     2941 tcgtcgacct cggcggcacc aacggcctgc gcgtcgccgg ccgccgcctc gctccgcgca
     3001 ccgagatccc ggtcggcgag cgcctgtgcg tcagcctcgg ccccttcgac ctcgaggtcg
     3061 tccacacccc cgaccgcgac cgtcaggccg tcagcggtgc tgtcgtcgaa cccggcccgg
     3121 tcgagctcga gcggctccac gccagcctcc gccggctgca cgccctgcac gcccctcacg
     3181 tcgctgctcg ccaggccttc gaggccgccc tcgccgacac gatccacacc ttccacggca
     3241 ccggcgacca gcctgcgatc cgccggatcc tcgccgagtt cccgaccgcc gaccacgccc
     3301 acctgatcct ccccacgccc cctcgcgcag ccccgcaacc tcccgcctca cctcgcccgc
     3361 cggcctcggt cgcccgccgc gaaccccaac ccctcggccc tcctaacctg tcccttcgaa
     3421 caccctcctc tgccccgggc ccccacgacc tgtcccctcg gacaccctcc tccacccccg
     3481 actccctcga cgcccacgac ctgtcccttc ggacaccctc ctgcgcccct cactccctcg
     3541 acacccctgc ccaccgccca gacgcccacg acctgtccct tcgaacacct ccgctcgacc
     3601 cctcccccgc gaacgctcgc cctccggctc ctcaccaaac catccccgcc gcgaccgcgc
     3661 ccgcccccgc cctcgccctg atcgccgacg ccgcccagac cctgctcccc gatcgccgcc
     3721 cgcccgccaa cctcgacgag gcccgccgct tcttcgatcg cctgatcagc accctgcgca
     3781 gcctcgccgc tggcgtcggc gcgctccagc acctgcgctt gcagcaggcc cgcgacctcg
     3841 acctctccct gctcgacctc gccaacccgc tgctcacccg caccagcgcc gacgagctgc
     3901 tcgccgacct gctcgcctgg cgcgaggac
//
LOCUS       DASWNJ010001535         7262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_559015, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001535 DASWNJ010000000
VERSION     DASWNJ010001535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7262
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559015"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VGB85_25505"
     CDS             complement(<1..384)
                     /locus_tag="VGB85_25505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HEY0137476.1"
                     /translation="MWNPFRRDDPEDDELAPRAGDRSWPTRLAWLVLVGGAAVLVLRC
                     GFATVVEIHGDGMAPTLLSGDHVLLVRGTWGLGAGDLVVYDPTPPAPPAPRTTPPPEI
                     PGPRERRGLPRNDGPRQPLRNAAVVD"
     gene            complement(359..1084)
                     /locus_tag="VGB85_25510"
     CDS             complement(359..1084)
                     /locus_tag="VGB85_25510"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEY0137477.1"
                     /translation="MTPSFRLLAITPPQGPVPPAIVSTWLGDGPGLALLLREPGRSAR
                     ELLAADGRLAAIRRACVAAGIPVLLSIGPEDLDLEIPPDIAGLHLRGDPDLPTLTRAR
                     ATRTRPVPSDIRDPRVIASAAWLGRSCHGAPQPGDALVDYTLFAPVFAPTTAQPGRPL
                     GSKTPAGLAALRAWTAAPKACVFALGGVGPATAAACLEAGAHGLAGIGVFFGEPGQVA
                     QDVAALREIVAAFDRHVEPVSPR"
     gene            complement(1162..1938)
                     /locus_tag="VGB85_25515"
     CDS             complement(1162..1938)
                     /locus_tag="VGB85_25515"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEY0137478.1"
                     /translation="MSDTQDTWTLAGRTWTSRLIVGTGKFKDAAETQAALLASGAEIV
                     TVALRRVDLKAKDNLLSWIDRDRYCLLPNTAGCYTADEAVRTLRLARELGIAEIVKLE
                     VIGDSRTLLPDNEETLKAARILLDEGFTVLPYCTDDPIVCRKLEEMGCAAVMPLAAPI
                     GSGLGIRNPHNIRLIVEQAKVPVIVDAGVGTASDAAIALELGCTAVLMNTAIAQARHP
                     ILMAEAMREAIIAGRKSLRAGRIPVRHYASASSPPTGMIE"
     gene            complement(1941..2144)
                     /gene="thiS"
                     /locus_tag="VGB85_25520"
     CDS             complement(1941..2144)
                     /gene="thiS"
                     /locus_tag="VGB85_25520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEY0137479.1"
                     /translation="MPTIVLNGEPHPIVEGTSVAALVAELGLSPQQVAVEKNREIVPR
                     GAHAQELLRDGDRLEIVTFVGGG"
     gene            2423..3103
                     /locus_tag="VGB85_25525"
     CDS             2423..3103
                     /locus_tag="VGB85_25525"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0137480.1"
                     /translation="METADVLACVEPFIAARPAELELLGRTAVRLRKAPGSVLVSEGQ
                     APDGLYIVLRGRVNLLRAADGGRDLILAALGPGEVFGEACAFPGVPMSTTAVAAVQSE
                     LLRVSPEALVEHLRREPETVLRLMRLQGDKLREVEAVASGLALCDVEERLRRTLARLA
                     RRQGRRGPASTGWILAPVPTQSELARMVGSCRETVSRTLSAMARSGLVSASGRRMILD
                     DRIVNEAA"
     gene            3249..4466
                     /gene="alr"
                     /locus_tag="VGB85_25530"
     CDS             3249..4466
                     /gene="alr"
                     /locus_tag="VGB85_25530"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY0137481.1"
                     /translation="MSAAHGPPRSNAPVTYNGPDRRGDDRRGRRPAPVVEIRPTYAHI
                     DLDALGHNLRTVQQHVGRRCRILAVVKADGYGHGAIEAARTFVDAGAWGLAVSLVEEG
                     VELRRVGVVAPVLVLGGVPPGSEDVIVHRSLTPVVWAREHLQLLAAAVQRSGAPPLPV
                     HLKVDTGMSRLGVLPEDLPELVDWLASEDGQHLQVEGLMTHFACADDANDTTSPVQIE
                     VFRACVATLASRGLHPPLRHVCNSAGLVRMPHAHFDMVRPGIALYGAGSGREVELPGL
                     RMAMSVHSRILGIRELPAGAKVSYGHSMTLTRDSRLGIVPVGYADGYARRMSGQAQVL
                     VRGHRCRVVGNITMDVSMIDVTDLPDAREGERVTLLGTQGRDCIDVYELAAWADVIPY
                     EITCGISKRVPRR"
     gene            4641..5783
                     /locus_tag="VGB85_25535"
     CDS             4641..5783
                     /locus_tag="VGB85_25535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137482.1"
                     /translation="MSREPELPRWRAEWPHAWIAPALAGAGWSLALAGSADLATQRSL
                     VFLGGPLVLLAGLHARLGDYLHGRGRARLAVLPVPATRHFAAGLVTHRRGFVVAAGFG
                     VLGVVLGAYAATGELLRGALLAGDFAWLCVLAMLVEPAIPALAAHAGRRFPDDHPIRQ
                     AQAQLGGGWTSPEAAVHLYAPALGLGLAALLAMPGQLGLGRIADGQALRAIHVALFAG
                     PVVVALIIRGVAPRVYAGGFFEAVAWLAEATRSLAGPPEPPARPAWVGRLAEPTRMFV
                     TQWLRLTPVPMLRLAALLGWGGYLLLRGARPTAPTVATLLGLAALWLVPLQTLARQRR
                     RNAALLAALPVPTAARTGRSPTLALAIAGVPVALVLALGLRWLVLA"
     gene            complement(5863..>7262)
                     /locus_tag="VGB85_25540"
     CDS             complement(5863..>7262)
                     /locus_tag="VGB85_25540"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY0137483.1"
                     /translation="AACVAPGPAATTALAGLLALAPGRAHAGPPVNLGTLTAPAPGPR
                     PQDRPAELVDLPPELNPTPTRPGPVQTWSLGQVMRTVEANHPRMRAEQLRRQVYNAGV
                     IAARTSIPNPVFLNDNGTAEFTYRVGFTQLLELGGKRRRRVELALARYALIDVDLRVL
                     AASLRAEARRTYMEAFFAQEREKTLIELILSVDELLRAADARPEEIPKSDVLEVRITR
                     LKARQALEQAKFEHFQADLRLNNLLGNRADVELVLTTPAGDRPPRWLVPSEDDWRHEF
                     LVAHEALVDQALRERPELLRVLREREILGKEERLVRANRIPNILASLGPDFVPQPGGG
                     VHTGVFWMLNVEVPLFNRQQGPLKDIDARRAQLAAGSDALRHDIARQVTDAVALACYS
                     QSQYRLYERELVPAADAVYADALAAFLANTAPFLVAVRAQEGRVLVRLEYLRSLAAYH
                     IALAGVDQALGFPAI"
BASE COUNT          909 a         2400 c         2928 g         1025 t
ORIGIN      
        1 gtcgacgacg gcggcgttgc gcagcggctg gcgcgggccg tcgttgcgcg gcaggccgcg
       61 gcgctcgcgg gggccgggga tctccggcgg cggggtggtg cggggggccg ggggcgcggg
      121 cggggtcggg tcgtagacga cgaggtcgcc ggcgccgagg ccccaggtgc cacgcacgag
      181 cagcacgtgg tcgccgctga gcagggtcgg ggccatgccg tcgccgtgga tctcgacgac
      241 ggtggcgaag ccgcagcgca gtacgagcac cgcggcgccg ccgacgagca cgagccaggc
      301 gaggcgcgtc ggccacgagc ggtcgccggc acgcggggcg agctcgtcgt cctcggggtc
      361 atcgcggcga aacgggttcc acatggcggt cgaacgccgc gacgatctcg cgcagcgccg
      421 cgacatcttg cgcgacctgg ccgggctcgc caaagaacac gccgatcccg gcgaggccat
      481 gcgcccccgc ctcgaggcag gccgcggcgg tcgccggccc gaccccgccg agggcgaaca
      541 cgcaggcttt gggcgcggct gtccaggccc gcagggcggc gaggccggcc ggtgtcttgc
      601 tgccgagcgg acgcccgggc tgcgcggtgg tcggcgcgaa gaccggggcg aacagggtgt
      661 aatcgacgag cgcgtcgccg ggctgggggg cgccgtggca cgagcggccg agccacgcgg
      721 ccgacgcgat gacgcgcggg tcgcggatgt ccgaagggac aggtcgggtc cgggtcgcgc
      781 gggcgcgcgt cagggtcggc aggtcgggat cgccgcgcag gtgcaggccg gcgatgtcgg
      841 gcgggatctc gaggtcgagg tcctcggggc cgatcgagag cagcacgggg atcccggccg
      901 ccacgcaggc gcggcggatc gcggccaggc ggccgtccgc ggccagcagc tcgcgcgcgc
      961 tgcggccggg ctcgcgcagc agcagcgcga gcccgggccc gtcgccgagc catgttgaaa
     1021 cgatggcggg cgggacgggg ccttgcggcg gggtgatcgc cagcaggcga aacgacgggg
     1081 tcatgttcag agcagcggat ccgtcatcag caggtcggcg ggcccgcggt gcgaggcgga
     1141 tccgtcatcc gcgggttccg gtcactcgat catgcccgtg ggggggctcg aggcgctggc
     1201 gtagtggcgc acggggatcc ggccggcgcg cagggacttg cggccggcga tgatcgcctc
     1261 gcgcatggcc tcggccatca ggatcgggtg gcgggcctgg gcgatcgcgg tgttcatcag
     1321 cacggcggtg cagccgagct cgagggcgat cgcggcgtcg ctggccgtgc cgacgccggc
     1381 gtcgacgatg acgggcacct tggcctgctc gacgatcagg cggatgttgt gcgggttccg
     1441 gatcccgagg ccgctgccga tcggggcggc gagcggcatg acggcggcgc agcccatctc
     1501 ctcgagcttg cgacagacga tcgggtcgtc ggtgcagtag ggcagcacgg tgaagccctc
     1561 gtcgagcagg atgcgcgcgg ccttgagggt ctcctcgttg tcgggcagca gggtccggct
     1621 gtcgccgatg acctccagct ttactatttc ggcgatgccg agctcgcgcg cgagtcgcag
     1681 ggtgcgcacg gcctcgtcgg cggtgtagca gccggcggtg ttgggcagca ggcagtagcg
     1741 gtcgcggtcg atccagctga ggaggttgtc cttggccttg aggtcgacgc ggcgcagggc
     1801 gacggtgacg atctcggcgc cgctggcgag cagggcggcc tgggtctcgg cggcgtcctt
     1861 gaacttgccg gtcccgacga tcagccgcga ggtccaggtg cggcccgcga gggtccaggt
     1921 gtcttgggtg tcgctcactt ttagcctccg cctacgaatg tgacgatctc caggcggtcg
     1981 ccgtcgcgca ggagctcctg cgcatgggcg ccgcgtggca cgatctcgcg gttcttctcg
     2041 acggcgacct gctgcggcga cagccccagc tcggccacca gcgcggcgac actggtgccc
     2101 tccacgatcg ggtggggctc accgttgagc acgatggtcg gcacgcgccg gaacgtacac
     2161 cgagaccgac agagaggcgc ggtagcgcga actccgcgga tgaaacttgg atcactgcgt
     2221 tgctttggac catcaggtgt gtcgatcgtg caacagacac acgcgaccca gatcacaggc
     2281 gcttttttgg gccaactgaa tggtttcaag gacttgggct ccgaaatgtc gaagcggtca
     2341 cttggcaggt tttgtgcttc gcagcacatc tcgtatgcac accgattcgg attccaagcg
     2401 ccccaacatc ccccttgagc gtatggagac cgcggatgtc ctggcttgcg tcgagccgtt
     2461 catcgcggcc cgcccggcgg agctagagct cctcggtcgt acggcggtcc gcctgcgcaa
     2521 ggccccgggc agcgtgctcg tcagcgaggg acaggccccg gacggcctct acatcgtcct
     2581 acgcgggcgg gtcaatctgc tgcgcgcagc cgacggcggc cgtgacctga tcctcgcagc
     2641 gctcggcccg ggtgaagtgt tcggcgaggc ctgtgccttc ccgggtgtgc cgatgtcgac
     2701 gaccgccgtc gcggcggtgc agagcgagct gctgcgcgtg tcccccgagg cgctggtcga
     2761 gcacctgcgc cgcgagccgg agacggtgct gcgcctgatg cggctgcaag gcgacaagct
     2821 gcgcgaggtc gaggcggtcg ccagcggcct ggccctgtgt gatgtcgagg agcgcctgcg
     2881 ccggaccctc gcccgcctgg cccgccgcca gggccgccgc ggcccggcca gcaccggctg
     2941 gatcctcgcc ccggtgccga cgcagtccga gctggcccgc atggtcgggt cgtgccgcga
     3001 gaccgtgtcg cgcacgctca gcgcgatggc ccgcagcggc ctggtcagcg ccagcggccg
     3061 ccgcatgatc ctcgacgacc gcatcgtcaa cgaagcggcc tgagtcggag aaacgtcgcg
     3121 gtgtgcaggc ccggggaccg tcggtctccg ggcttcgtcg tcggtgggaa tgccggcgag
     3181 ggcggcggcg ttgcccggcc gtggcgaccc ggatcccgca ccacgacaga gatgatagcc
     3241 tcccggcgat gagcgcagcg cacggccctc cgcgttcgaa cgcgccagtg acgtataacg
     3301 gccccgaccg gcgtggcgac gaccgccgcg gccgccggcc cgcgccggtc gtcgagatcc
     3361 gcccgaccta cgcacacatc gacctcgacg cgctcggcca caacctgcgc acggtccagc
     3421 aacacgtcgg ccgccgctgc cggatcctcg cggtggtcaa ggccgatggc tacggacacg
     3481 gcgcgatcga agcggcgcgg accttcgtcg acgcgggcgc gtgggggctg gcggtcagcc
     3541 tggtggagga gggcgtcgag ctgcggcggg tcggggtggt ggcgcccgtg ctggtgctcg
     3601 gcggggtgcc gcccggatcc gaggacgtga tcgtgcaccg atcgctgaca ccggtggtat
     3661 gggcgcgcga acacctgcag ctgctcgcgg ccgcggtcca gcgcagcggg gcgccgccgc
     3721 tgccggtcca cctcaaggtc gataccggga tgtcgcgcct cggtgtgctg cccgaagacc
     3781 tgccagagct ggtcgactgg ctcgccagcg aggacggaca gcacctgcaa gtcgaggggc
     3841 tgatgacgca cttcgcgtgc gccgacgatg cgaacgacac gaccagcccg gtgcagatcg
     3901 aggtgttccg cgcgtgcgtg gcgacgctgg cgagccgcgg cctgcacccg ccgctgcgcc
     3961 acgtgtgcaa cagcgccggg ctggtcagga tgccgcacgc acacttcgac atggtgcgcc
     4021 cgggcatcgc gctgtacggc gcaggctcgg gccgcgaggt cgagctgccg gggctgcgca
     4081 tggcgatgag cgtgcactcg cggatcctcg ggatccgtga gctgccggcc ggcgccaagg
     4141 tgtcgtacgg gcacagcatg acgctgaccc gcgactcgcg gctcggcatc gtgcccgtcg
     4201 gctacgccga tgggtacgcg cgacgcatga gcggccaggc ccaggtgctg gtccgcggac
     4261 accgctgccg cgtggtcggc aacatcacga tggatgtctc gatgatcgat gtcaccgacc
     4321 tgccggacgc tcgtgaaggc gagcgcgtga cattgttggg gacgcagggg cgtgactgca
     4381 tcgatgtgta tgagctggcg gcgtgggctg atgtgatccc gtatgaaatt acgtgcggga
     4441 tctcgaagcg tgtgcctcga cggtaggggg gccgcggacg gtgtggcacg ggtcgggcgt
     4501 gccgccgagc gacacgacgc gtcgggccgc gcctcgcacg gtccggaaag gtgggatccg
     4561 cccgcggcat ggcgttgcgt gggcggcggg gcgccaggca gggagctggc ggtggggcgt
     4621 ggctgtggca tcctcgcggc gtgagcaggg agccggagct gccgcggtgg cgcgccgagt
     4681 ggccgcacgc ctggatcgcg ccggcgctgg cgggcgcggg gtggtcgctg gcgctggccg
     4741 gcagcgcgga cctggcgacc cagcgcagcc tggtgttcct cggtgggccg ctggtgctgc
     4801 tcgccgggct gcacgcgcgg ctcggcgatt atctgcacgg gcgcgggcgg gcccggctcg
     4861 cggtgttgcc ggttcccgcg acgcggcact tcgcggcggg gctggtcacg caccggcgtg
     4921 gcttcgtggt cgcggcgggg ttcggggtgc tcggtgtggt gctcggggcg tatgcggcga
     4981 cgggggagtt gttgcgtggg gcgctgctgg ccggtgactt cgcgtggctg tgcgtgctgg
     5041 cgatgctagt cgagccggcg atcccggcgc tggcggccca cgcggggcgg cgcttcccgg
     5101 acgaccaccc gatccgccag gcgcaggcgc agctcggcgg cggatggacg tcgccggagg
     5161 ccgcggtgca cctgtatgcg ccggcgctcg ggctcgggct cgcggcgctg ctggcgatgc
     5221 cggggcagct cgggctcggg cggatcgcgg atggccaggc gctgcgagcg atccacgtcg
     5281 cgctgttcgc cgggcccgtg gtcgtcgcgc tgatcattcg tggggtcgcg ccgcgcgtgt
     5341 acgcgggcgg gttcttcgag gcggtcgcgt ggctggccga ggcgacgcgc agcctcgcgg
     5401 gaccacccga gccgcccgcc cgaccggcgt gggtcggcag gctcgcggag ccgacgcgaa
     5461 tgttcgtgac gcagtggctg cgcctgacgc ccgtgcccat gctgcggctc gccgcgctgc
     5521 tcggctgggg cggctatctg ctgctccgcg gcgcgcggcc gacggccccg acggtggcga
     5581 cgctgctcgg cctcgctgcg ctgtggctgg tgccgctgca gacgctcgcg cgccagcgcc
     5641 gacgcaacgc agcgctgctc gccgcgctgc cggtgcccac ggcggcccgc acgggccgca
     5701 gcccgacgct cgcgctggcg atcgcgggcg tgccggtcgc gctggtgctc gcgctcgggc
     5761 tgcggtggct cgtgctcgcc tgagcctgtc cgataggaca tgtcgggcgc ggaccgcgcc
     5821 acgccgttcg ccgagcctgt ccgcgaggac atgtcccgca ggtcagatcg ccgggaagcc
     5881 gagcgcctga tcgacgccgg cgagggcgat gtggtaggcg gcgaggctgc gcaggtactc
     5941 gaggcggacg agcacgcggc cctcctgcgc gcgcacggcg acgaggaagg gggcggtgtt
     6001 ggcgaggaag gcggcgaggg cgtcggcgta gacggcgtcg gcggcgggta cgagctcgcg
     6061 ctcgtagagg cggtactggg actgcgagta gcaggcgagc gcgaccgcgt cggtgacctg
     6121 gcgggcgatg tcgtggcgca gggcgtcgct gccggcggcg agctgggccc ggcgcgcgtc
     6181 gatgtccttg agcgggccct gctgccggtt gaacagcggg acctcgacgt tgagcatcca
     6241 gaacacgccg gtgtgcacgc cgccgccggg ctgcgggacg aagtcggggc cgaggctggc
     6301 gaggatgttg gggatccggt tggcgcggac caggcgctcc tccttgccga ggatctcgcg
     6361 ctcgcgcagg acccgaagca gctcggggcg ctcgcgcagg gcctgatcga cgagcgcctc
     6421 gtgggcgacc aggaactcgt ggcgccagtc gtcctcgctc gggaccagcc agcgcggcgg
     6481 ccgatcgccc gcgggcgtgg tcagcacgag ctcgacgtcg gcccggttgc cgagcaagtt
     6541 gttgaggcgc aggtcggcct ggaagtgctc gaatttggcc tgctcgagcg cctggcgagc
     6601 cttgagacgg gtgatgcgga cctcgagcac gtccgacttg gggatctcct cggggcgcgc
     6661 gtcggcggcc cgcagcagct cgtcgacgct gaggatcagc tcgatcaggg tcttctcgcg
     6721 ctcctgcgcg aagaaggcct ccatgtacgt gcgtcgggcc tcggcgcgca ggctggccgc
     6781 gagcacccgg aggtcgacgt cgatcagggc gtagcgcgcg agcgcgagct cgacgcgacg
     6841 ccggcgcttg ccgccgagct cgagcagctg ggtgaagccg acgcggtagg tgaactcggc
     6901 ggtgccgttg tcgttgagaa acaccgggtt ggggatcgat gtccgcgccg cgatcacgcc
     6961 ggcgttgtag acctggcggc gcagctgctc ggcgcgcatg cggggatggt tggcctcgac
     7021 ggtgcgcatc acctgaccca aagaccaggt ctgtaccggg cccgggcgcg tcggcgtcgg
     7081 gttgagctcg ggcgggaggt cgacgagctc ggccgggcgg tcctgcgggc gcgggccggg
     7141 tgcgggggcg gtgagggtgc cgagattgac cggcggcccg gcgtgcgcgc ggcccggggc
     7201 gagcgcgagc aggccggcga gggcggtcgt ggcggcaggg cccggcgcga cgcaggcagc
     7261 cc
//
LOCUS       DASWNJ010001536         4351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_97717, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001536 DASWNJ010000000
VERSION     DASWNJ010001536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97717"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VGB85_25545"
     CDS             complement(<1..229)
                     /locus_tag="VGB85_25545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137484.1"
                     /translation="MAELRSKTRRASNYGVGARLVVEKYRGGAVVPENPGEQRRVAAI
                     FPAVNKLDAVSVRIDDRGQFQAENIPGVAQTG"
     gene            233..>4351
                     /locus_tag="VGB85_25550"
     CDS             233..>4351
                     /locus_tag="VGB85_25550"
                     /inference="COORDINATES: protein motif:HMM:NF023677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY0137485.1"
                     /translation="MAAIRTEAAGAPVRTTELRYATSHAVPSSQLTAIATVAADGTAL
                     PVLRFEYTAPGDALARFDLADGPALDPTAEGRAWVDVDGDALPDLLEGQPGAWRHRKN
                     LGGQALGPWIVLKNAPAAAIGPTARFADVTGDGVQDLLDRPGQGDAYAFLGGGAAPFN
                     KSATLALDVSFDLADPRLALVDLNLDGRIDILRHDDGDGWIWVQRWDGPGFNPAEPVP
                     PPPAGLHLGEPGVQLADMDGDRLPDLVRILPTDARILVAPSAGLGFFDEATEMAGVPK
                     MSETERWEIADVSGDGAGDLVRIGHREVGLWINQLDGTFASVGAHAWPELDADEVVIL
                     SDVDGTGTVDLLRVDTDGSQPWRYFSPIVQRPGLLQAQENGLGYRATMKYQPAAQLAA
                     ADAAAGAPWSSTPPAPAPVLIATVESDGGEWTRTTNYHPRDGWHDPERGELRGFAEHA
                     HTRDGDNYSSAATVTRRYDLGRQDEAHGLQLLEEITSGPNGALVRELHTLEAEELAPG
                     VRTARRIATDTFHLEAGPEQAAARVRTEWDYDAAGNVTEERALGRVDNKSGADLPGDE
                     RIITTTHAGPIDPGGPRNLAAEIVVTDGDGVQVSATRTYYDGEAEKGLALGQVARRGL
                     VARAETWTDCENWLTSLRQAHDAHGNTTRIRDARDGTLERRYDATGLFPVEERLVLDD
                     GALVTTATWDPAHGHPRTVTTPNSATAEAIYDGLGRLTAEIQPGDSAERPTIRYRYFL
                     DGSTERPAILTERRRISREDAVDVEVTQLDGLGRPQARVTPDDVGTAAVLAEGLFYDA
                     AGAVAERTEGVPLPVAALTPGIHVELPNAPRTQTWRDALGRVTATRDADGFDTLTRYS
                     PLAQDEFDNEDIHSAPPYSTTPERREFDGLGHVIAQRAILPDRSITHRYEHDAAGRLR
                     AHIDPAGHASRYTYDGAGRLLRIDAPDAGTIRQFFDATGQITERRDATGARVTWTHDR
                     LGRVLTERAHDPAGKQLGEVRYTYDRGRDPAATYSRGKLTAVEDAAGRVDFDHDIRGR
                     IVRVARQFAAHAGPITVSQSTEYDAQDRIVREIFPDGAELERDYTARGLAAPLAGWLA
                     AATYDPRGRWTALELASGVRTTRALDHTGRLQAHRVTQDTDDLLNFTHTYDAAGLLAQ
                     TQDHLVADQSQRFAYDDLRRLTRAQGSYGVQTWAYSDDDNITRSGEASYSYAGPQPHA
                     VTAARGQSFTYDAAGNLAAVAGKGPVPAGTWRTNPQGRVDSFTAANGDRTEHIYDHAG
                     TEAIRREYTASGSLTHETLYFSKQVEVRDGQLVRWIVWNGERIAESAAKLPETGYAPQ
                     PLSGAALGALLVVALALLRRRRAAWPRMDRPLRFAGQLALFVLASVSCQSGDA"
BASE COUNT          683 a         1769 c         1360 g          539 t
ORIGIN      
        1 cgcccgtctg ggccacgccg gggatatttt ccgcctggaa ctgtccgcgg tcgtcgatcc
       61 gcacggaaac cgcgtcgagt ttatttacgg ccgggaaaat ggcagcgacg cgccgctgct
      121 cacccggatt ttctggaacg acggcgccgc ctcggtattt ttcgactacg aggcgcgccc
      181 cgaccccgta attactcgcg cgccgggttt tcgagcggag ctccgccatc gcttggccgc
      241 catccgcacc gaggccgcgg gcgcgccggt gcgcaccacc gagctccgct acgcgacctc
      301 gcacgcggtc ccgagcagtc agctcaccgc gatcgccacc gtcgccgccg acgggaccgc
      361 gctcccggtc ctgcgcttcg agtacacggc cccaggagac gcgctggcgc gcttcgacct
      421 cgcggacggg cccgcgctcg accccaccgc cgaaggccgc gcctgggtcg acgtggacgg
      481 cgacgcgctg cccgacctgc tcgaaggcca gcccggcgcc tggcgccacc ggaaaaacct
      541 cggcggccag gcgctcggcc cctggatcgt cctcaaaaat gcgcccgccg ccgcgatcgg
      601 acccaccgcg cgcttcgccg acgtgaccgg cgacggcgtg caggacctgc tcgacaggcc
      661 ggggcagggt gacgcctacg catttctcgg cggtggcgcg gccccattta ataaatccgc
      721 cacgctggcg ctcgacgtca gcttcgatct cgccgatccg cgcctcgcgc tcgtcgatct
      781 caacctggac ggccggatcg acatcctgcg ccacgacgac ggcgacggct ggatctgggt
      841 gcaacgctgg gacggcccgg gatttaatcc cgccgagccc gtccccccgc cgcccgctgg
      901 cctgcacctg ggcgagccgg gtgtgcagct cgcggacatg gacggcgacc gcctgccgga
      961 cctcgtgcgc atcctgccga ccgacgcccg cattctcgtg gctccgagcg ccggcctggg
     1021 ctttttcgac gaggcgaccg aaatggccgg cgtcccgaaa atgtcggaga ccgagcgctg
     1081 ggaaatcgcc gacgtgtccg gcgacggggc gggcgacctc gtgcggatcg gccaccgcga
     1141 ggtcgggctg tggattaatc aactggacgg cacttttgcg tccgtcggcg cgcatgcctg
     1201 gcccgagctg gacgccgacg aagtcgtgat tttgagcgac gtcgacggca ccggcaccgt
     1261 cgatctgctg cgcgtcgaca ccgacggctc gcagccctgg cgctactttt cgccgatcgt
     1321 gcagcgcccc ggcctcctgc aggcgcagga aaatggcctc ggctaccgcg cgaccatgaa
     1381 ataccagccc gcggcgcagc tcgccgccgc ggatgcagcc gcgggcgccc cctggtccag
     1441 caccccgccc gcgcccgcgc cggtcctgat cgccaccgtc gagagcgacg gcggcgagtg
     1501 gacgcggacc acgaattacc acccgcgcga cggctggcac gaccccgagc gcggcgagct
     1561 gcgcggcttt gccgagcacg cccacacccg cgacggcgac aactacagca gcgccgcgac
     1621 cgtcacccgc cgctacgacc tgggccgcca ggatgaagcc cacggtctcc aactcctcga
     1681 agaaatcacg tccggcccga atggcgccct ggtccgcgag ctgcacacgc tcgaggccga
     1741 ggagctggcg ccgggcgtcc gcacagcgcg gcgcatcgcc accgacacat ttcacctcga
     1801 agctggcccc gagcaggccg ccgcgcgcgt acgcaccgaa tgggattacg acgccgccgg
     1861 caacgtcacc gaggagcgcg ccctgggccg cgtcgacaat aaatccgggg ccgacctccc
     1921 gggcgacgaa cgcatcatca cgactaccca cgccgggccg atcgaccccg gcggcccgcg
     1981 caacctcgcc gccgagatcg tcgtgaccga cggcgacggc gtgcaagtct ccgccacgcg
     2041 cacctactac gacggcgagg ccgaaaaggg actcgcgctc ggacaggtcg cccgccgcgg
     2101 cctggtcgcg cgcgccgaga cctggaccga ctgcgaaaac tggctcacgt cgctgcgcca
     2161 ggcccacgac gcgcacggca acaccacccg catccgcgac gccagggacg gcaccctcga
     2221 gcgccgctac gacgccactg gcctgtttcc tgtcgaagag cgcctcgtgc tggacgacgg
     2281 cgcgctcgtc accaccgcca cctgggatcc cgcacatggc cacccgcgta ccgtcactac
     2341 gccgaacagc gcgaccgcgg aggccatcta tgacggcctg ggccgtctca ccgccgagat
     2401 ccagcccggc gacagcgccg agcgcccgac gatccgctac cgctattttc tcgacggcag
     2461 caccgagcgc ccagcgatcc tcaccgagcg gcgccgcatc agccgcgagg acgccgttga
     2521 cgtcgaggtc acgcagctcg acggcctcgg ccgcccccaa gcccgcgtca caccggacga
     2581 cgtcggcacc gccgccgtgc tcgccgaggg cctgttctat gacgccgctg gcgccgtcgc
     2641 cgaaaggacg gagggcgtgc cgctccccgt cgccgcgctc acgccgggga tccacgtcga
     2701 gctgcccaat gcgccgcgca cccagacctg gcgcgacgcg ctcggccgcg tcaccgccac
     2761 gcgcgacgcc gacggcttcg acaccctgac ccgctacagc ccgctcgcac aggatgaatt
     2821 cgacaacgaa gacatccact ccgcgccgcc ctacagcacc accccggagc gccgcgaatt
     2881 cgacggcctc ggccacgtga tcgcccaacg ggcgatcctc cccgaccgca gcatcaccca
     2941 ccgctacgag cacgacgccg caggccgcct gcgcgcgcac atcgatcccg ccggtcatgc
     3001 gagccgctac acctacgacg gcgcgggccg cctgctccgc atcgacgcgc cggacgccgg
     3061 caccatccgg caattcttcg atgctaccgg ccagatcacc gagcgccgcg acgccaccgg
     3121 cgcgcgcgtc acctggaccc acgaccgcct cggccgcgtg ctcaccgagc gcgcccatga
     3181 ccccgccggc aagcaactcg gcgaggtccg ctacacctac gatcgcggcc gggatcccgc
     3241 cgcgacctac agccgcggca agctcaccgc cgtcgaggac gcggccgggc gcgtcgactt
     3301 cgaccacgac atccgcggcc gtatcgtccg cgtggcccgt caattcgccg cgcatgccgg
     3361 cccgatcacc gtctcgcaga gtaccgagta cgacgcccag gaccgcatcg tccgcgaaat
     3421 cttccccgac ggcgccgagt tggagcgcga ctacacggcc cgcggtctcg cggcgccgct
     3481 cgccggctgg ctcgccgccg ccacctacga cccgcgcggc cgctggaccg cgcttgagct
     3541 cgccagcggc gtccgcacga cccgcgcgct cgaccacacc ggccgcttgc aggcccatcg
     3601 cgtcacccag gacacagacg acctcctgaa ttttacgcac acctacgacg ccgccggcct
     3661 gctcgcacag acccaggatc accttgtcgc cgatcagtcg cagcgcttcg cgtacgatga
     3721 tctccgccgc ctcacccgcg cgcagggctc gtacggcgtc cagacctggg cctactccga
     3781 cgacgacaac atcacgcgca gcggcgaagc ctcgtacagc tatgcgggcc cgcagccgca
     3841 cgccgtcacc gccgcgcgcg gccagagctt cacttacgac gccgccggca acctcgccgc
     3901 cgtcgcgggc aaggggcccg tccccgccgg cacctggcgc accaacccgc agggccgcgt
     3961 cgactcgttc accgccgcga acggcgaccg caccgagcac atctacgatc atgccggcac
     4021 cgaggcgatc cgccgcgagt acaccgcgag tggcagcctg acgcacgaga ccctctactt
     4081 ctcaaagcag gtcgaggtcc gcgacggcca gctcgtccgc tggatcgtgt ggaacggcga
     4141 gcgcatcgcc gagagcgccg cgaagctccc cgagaccggc tacgccccgc agccgctctc
     4201 cggcgccgcc ctgggcgcac tcctcgtcgt cgcactcgcg ctgcttcgcc gccgccgcgc
     4261 cgcatggccc cgcatggacc gacccctgcg ctttgccggc cagctcgccc tgttcgtgct
     4321 cgcctcggtg agctgccagt ccggcgacgc c
//
LOCUS       DASWNJ010001537         1698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_449337, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001537 DASWNJ010000000
VERSION     DASWNJ010001537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449337"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1319
                     /locus_tag="VGB85_25555"
     CDS             <1..1319
                     /locus_tag="VGB85_25555"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY0137486.1"
                     /translation="PVAPAPQADPAPVPPPVSVAPASAPAATTASPAAAPSRFVPPTD
                     AARLAEAPVDGGKARWRPGVGIEVSSADGRFSLQFNALSQLQAVAHFTPEIPGMDGGE
                     ATAAKNDFNLVFRRARLVFGGNLFSKNIKYKIQLTASPIELGWRSGTITRSPILDWYF
                     TFDRLRDATFQVGQYKVPYNHQRMLRVTGMQFVDRSAANNEFTMDRDIGLDIRSKDVG
                     GLGKLRYYAGVYLGDGIALHGPSDFGLAYVGRVEVLPFGQYDDLEEGDHDRLTRPRML
                     IGGAFAYIDKDPHDQHGFGGQIPADGRKASTLNATADMNFRYAGFSFEGAFFWRKAVK
                     MTPVTVLNTMGNPVAAVAPRDGLGYFLQAGYLLPRLPLELGARWGQVRGQGDATSLKD
                     QNEVGGVFNYFFARHWLKLQLDYLHLWNNEIRYGTDQVRLQLQAMF"
BASE COUNT          267 a          623 c          542 g          266 t
ORIGIN      
        1 tccctgtcgc tcccgcgccg caagccgacc ccgcaccggt cccgccgccc gtgtccgtcg
       61 ccccggcgtc ggcgcccgct gccacgaccg cgagccccgc tgcggccccg tcgcgcttcg
      121 tgccgccgac cgacgccgcg cgcctggccg aggccccggt cgacggcggc aaggcgaggt
      181 ggcggccggg cgtcggcatc gaggtcagca gcgccgacgg tcgcttctcg ttgcagttca
      241 acgcgctctc gcagctgcag gcggtcgccc acttcacgcc cgagatcccc ggcatggacg
      301 gtggcgaggc gacggcggcg aagaacgact tcaacctcgt gttccgccgc gcgcgcctgg
      361 tgttcggcgg caacctgttc tcgaagaaca tcaagtacaa gatccagctg accgcgtcgc
      421 cgatcgagct cgggtggagg agcggcacga tcacgcgctc gccgatcctc gactggtact
      481 tcaccttcga tcgcctccgc gacgcgacct ttcaggtcgg ccagtacaag gtgccctaca
      541 accaccagcg catgctgcgg gtcaccggca tgcagttcgt cgaccgctcg gcggccaaca
      601 acgagttcac gatggaccgc gacatcggcc tcgacatccg ctccaaggat gtaggcgggc
      661 tcggcaagct ccgctactac gcgggagtct acctcggcga cggcatcgcg ctgcacggcc
      721 ccagcgactt cggtctcgcc tatgtcggcc gcgtcgaggt gctgcccttc ggtcaatacg
      781 acgatctcga ggagggcgac cacgaccgcc tcaccagacc tcgcatgctg atcggcggcg
      841 ccttcgcgta catcgacaag gacccgcacg accagcacgg cttcggcggg cagatccccg
      901 ccgacgggcg caaggcctcg acgctcaacg ccaccgcgga catgaacttc cgctacgccg
      961 gcttctcctt cgagggcgcg ttcttttggc gcaaggccgt gaagatgacg ccggtcaccg
     1021 tgctgaacac gatgggcaac ccggtcgccg ccgtcgcgcc gcgcgacggc ctcggatact
     1081 tcctgcaggc cggctacctg ctgccgcgcc tgccgctgga gctcggcgcg cgctggggtc
     1141 aggtccgcgg gcagggcgac gcgaccagcc tcaaggacca gaacgaggtc ggcggcgtct
     1201 tcaattattt cttcgcccgc cactggctca agctccagct cgactacctg cacctgtgga
     1261 acaacgagat ccggtacggc accgaccagg tccgcttgca gctgcaggcg atgttctagg
     1321 cgcgcgcgac ccggatcgcc gagcctggcg tctccagccc gcagcggcgg cgagcgccga
     1381 agtacatacg acccgtgagt tggctcgcgc gatcacaccg cgtggactcg ggcccccatt
     1441 gatcgggtcg gctggtgacg cgtgcatcgg agcacttccc tcttttgtcg cacgcatggc
     1501 ctgtgcgacg atgacgacga acttgtgcgc ggacggacac cgcggcgaaa gagtcacatc
     1561 atgctgagca ctaccccttt tcgagccacc cgaatcttac tttccctggg tctgtgcagc
     1621 gggctctcgc tcgcctgcgg cgacagcggc agcgacgacg actcgaccac gacggtgacg
     1681 gcgtccggcg gctcggtg
//
LOCUS       DASWNJ010001538         6498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_592925, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001538 DASWNJ010000000
VERSION     DASWNJ010001538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6498
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592925"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /locus_tag="VGB85_25560"
     CDS             <1..1175
                     /locus_tag="VGB85_25560"
                     /inference="COORDINATES: protein motif:HMM:NF015376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HEY0137487.1"
                     /translation="GPALALVAVLLAAGGTWALWPVAAPPTPVTTMAARTAAEATRPT
                     AAPTPTRPRTLVHHEVIPGETIADIAARYGVSASQLIADHDLNPDLPPPPGTELVFAA
                     IDPPLPKLRLRHTVEAGDTLTSLGERFDVGVELLRRYNPGFTGEVTEGAQLIVWVDPQ
                     IERRHDEAVRLSFPVAPDALSIGAPTLGSLERGIQLPASPYYERVHPALQYGSSHAIQ
                     YLQTAIARFRQLYRYQGVLVVSDLSKNGGGPLPPHKSHQAGRDVDIWLPALKGTYQGR
                     HLADDRKPKFAEINWYAAWGLVESLLATGEVQYVFLDISLHPKLYEAGQRLGAAPDML
                     AQLQWQPPDADPELARRAKYNAPVRHAAEHNGHIHVRFKCGPNEPRCTHKVDVEDP"
     gene            complement(1191..1565)
                     /locus_tag="VGB85_25565"
     CDS             complement(1191..1565)
                     /locus_tag="VGB85_25565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137488.1"
                     /translation="MELRARMDAEGMSRLEEHLDLGLAGVVVGLWIGFLGSLRQPSGE
                     LGWFVSLAALHLLPLPIVLVGSETDSKQFFGWVAFGGLALILGLPHHAWPAGRRAWPR
                     VVTTVAVGVGLGVLFYARRSLA"
     gene            complement(1644..1967)
                     /locus_tag="VGB85_25570"
     CDS             complement(1644..1967)
                     /locus_tag="VGB85_25570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137489.1"
                     /translation="MSGTSETSETSGTSAGTTDETTDGTTGAIDFGRCGWYAQGQQYT
                     CSVFGGWPGHEDPAMVYPIACPDGLEKGDDCARWGIDAHGCCLADAYAICEGGGVFVE
                     VCYDR"
     gene            complement(2458..5109)
                     /locus_tag="VGB85_25575"
     CDS             complement(2458..5109)
                     /locus_tag="VGB85_25575"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0137490.1"
                     /translation="MTVPDTLIADTLASDSAGRAFVSERDDTGTATGGGRARQDDPGA
                     SPQRVGRYHVLGRIGSGGMGVVYSAYDPDLDRRVAVKLLRTDHGGRDSQLRLLREAQA
                     LARLSHPNVVQIYDTDAFGEQVFIAMEFIQGVDLHGWLTAAERSIDEILRVFLDAGRG
                     LAAAHDAGLVHRDFKPDNVLVGADGRVCVADFGLARDGSEEARAPDAGQAARPLDMSL
                     TATGALLGTPTYMSPEQHEGRVADARSDQFSFCVALWEALYGGRPFRGDSHAQIALAV
                     TAGRITEPPADTRGPRRLIAVLRRGLVVAPAGRYPSMHALLAALAPRPRTRWRWLTAV
                     GLAGLTGGAGLALADWRAREANACSGGAREIAAGWDEADHAVVTEALATGRDPGLAGR
                     VVDGLDAYADGWVAAHRDACLVHRRDEQSSALFDARTRCLQQRRSALASAVDLLTAPA
                     GPPPDAAQLVARLPAIASCSDVAVVMAEVAAPDDPALAAAVAEVEGALISARNQLHAG
                     DLQGAERVAAAAVLEARRVGFRPTLAGALLTLATIEMNQARGAAAAGHLEEATAIAVA
                     TRQDAIAAESFSRAIFVEVVQNSGRVELALAQVPLARGFAERLPDPAAALARVASNEG
                     TAHLARGDRARAAEAFARAVAESDRAVDVDPIDRAYYLASLAHTTEDPQRRDLLFARS
                     EAGLTQLLGSQHPVVLRQRLMRARLGPDLAAARDLLRATCPALRERIPEDADSCSLCN
                     YTLGHLELRLGDVAQAGAALVATRRCVEGQPDDADGQLQAHGAAALVASLAGDHAAAV
                     AAVDASIATFAAYRDRPWIAADLAELELTRGRALLALGRRDEAITAFERAVTDLAAKH
                     PMDHLTLPRLWLADARALLASTRGEGP"
     gene            complement(5118..6107)
                     /locus_tag="VGB85_25580"
     CDS             complement(5118..6107)
                     /locus_tag="VGB85_25580"
                     /inference="COORDINATES: protein motif:HMM:NF039450.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADYC domain-containing protein"
                     /protein_id="HEY0137491.1"
                     /translation="MSPIHSLVRLPAPRVLLSLLPLVLAGGCYADEADDPGLHEFRTT
                     GSCTTCGLGFPNSPEINDFEIPELHLDGALNSDGVQLLRIRSATGSYPLHVIDEQFAV
                     VLPNQTLSGADLLGWKIDLDSPDGHYLLEIAGVDTIPSLADGAPPMVRYALKYESHLV
                     PGEILNVCPEATPDETVVTLIRDETYDREHKLVVPDQYGWFTLACADQAAYKMQRMNY
                     GPNADFNGTPSPATVDERQATLKMITADYCGKGFSFTAQGTPILWRDADKTVGIEPPA
                     NAALEAYWSADGALCLDRPRLVGSQDVESECVLPPCSKIDVEDPRIVWTTHTN"
BASE COUNT          943 a         2509 c         2179 g          867 t
ORIGIN      
        1 tcggccctgc gctcgcgctc gtcgccgtcc tgctcgccgc cggcggcacc tgggcgctgt
       61 ggcccgtcgc cgcgccgccg accccggtca ccacgatggc ggcgcgcacc gccgccgagg
      121 cgacgcgtcc gaccgccgcg ccgaccccga cgcgcccgcg caccctcgtg caccacgagg
      181 tcatccccgg cgagacgatc gccgacatcg ccgcgcgcta cggcgtgtcc gccagccagc
      241 tgatcgccga ccacgacctc aaccccgacc tgccgccgcc ccccggcacc gagctggtgt
      301 tcgccgcgat cgacccgccc ctgcccaagc tgcgcctgcg ccacaccgtc gaggccggcg
      361 acaccctcac cagcctcggc gagcgcttcg acgtcggcgt cgagctgctt cgccgctaca
      421 accccggctt caccggcgag gtcaccgagg gcgcgcagct gatcgtgtgg gtcgacccgc
      481 agatcgagcg ccgtcacgac gaggccgtgc gcctcagctt ccccgtcgca cccgacgcgc
      541 tctcgatcgg cgctccgacc ctcggctcgc tcgagcgtgg gatccagctg ccggcgagcc
      601 cgtactacga gcgcgtgcac ccggccctgc aatacggatc cagccacgcg atccagtatc
      661 tacagaccgc gatcgcacgg tttcgccagc tctaccgcta tcagggcgtg ctcgtcgtct
      721 ccgacctcag caaaaacggc ggtggtccgt tgccgccgca caagtcgcac caggccgggc
      781 gcgacgtcga catctggttg cccgcgctca agggcaccta tcaagggcgc cacctcgccg
      841 acgaccgcaa gcccaagttc gccgagatca actggtacgc cgcctggggg ctcgtcgagt
      901 cgctgctggc caccggcgag gtccagtacg tgttcctcga catctcgctg cacccgaagc
      961 tctacgaggc cggccagcgc ctcggcgccg cgccggacat gctcgcgcag ctgcaatggc
     1021 aaccgcccga cgccgacccc gagctcgccc gccgcgccaa atacaacgcc cccgtgcgcc
     1081 acgccgccga gcacaacggc cacatccacg tccgcttcaa gtgcggcccg aacgagccgc
     1141 gctgcaccca caaggtcgac gtcgaggacc cctgagcgcg tggccgcggc tcaagccagc
     1201 gagcgccggg catagaacag cacaccgagc ccgacgccga ccgccacggt cgtcacgact
     1261 cgcggccatg cgcgtcgccc cgcgggccac gcgtggtgcg gcaggccgag gatcagcgcc
     1321 agcccgccga acgcgaccca gccgaaaaat tgcttcgaat cggtctcgct gccgaccagc
     1381 acgatcggca gcggcagcag gtgcagcgcc gccagcgaga cgaaccaccc gagttcgccg
     1441 ctcggctgcc gcagactgcc caggaacccg atccacaggc ccaccacgac ccccgccagc
     1501 ccgaggtcga ggtgctcctc gagccgactc atcccctcag cgtccatgcg cgcgcgcagc
     1561 tccatccgtc gaccttacgc cttttcacga gccgcgacag cctcacagac ccctccgacg
     1621 tcggctgcgt gaaagcggtc gcctcagcgg tcgtaacaca cctcgacgaa cacgccccca
     1681 ccctcgcaga tcgcatacgc gtcggccagg cagcagccgt gcgcgtcgat cccccaccgc
     1741 gcgcagtcgt cccccttctc gagtccatcc gggcacgcga tcggatagac catcgccggg
     1801 tcctcgtgcc cgggccagcc cccgaacacc gagcacgtgt actgctgccc ctgcgcgtac
     1861 cagccgcagc gcccgaagtc gatcgcgccc gtcgtgccgt ccgtcgtctc gtccgtcgta
     1921 cccgcgctcg ttccgctcgt ctcgctcgtc tcgctcgtcc cgctcatctc gcttgtccca
     1981 ctcgtcgcgg tcgcgcccgt ggtatccggt ccgctcgtcc cgctgtcctg cgtgctgctc
     2041 tcgccgccgt cagcgttcgt aaccgccgag gtcgaagatc cggaggtcgt cggtgtacct
     2101 gtcgtggtcg tcgcaccgcc agtcgttgcc gccgacaaac caccactcgt gccggtgccg
     2161 ctgccggccc cggtcgtcgc gccgccgtcg gagttgcccc gggggacgac gagcaggccg
     2221 cgagcgagcc cgcgaggagc acatgaagcg ttcgtgaaag catcgaggaa caacgttaca
     2281 cgcaaccgcc tgcccacgcc cgatccggcg tcgctcggcc gaaagtagag cttacgctcg
     2341 tgctgtcact gtccgccgcc gagcgcatcc acgacgaacc tcgatggagc ttcaccgata
     2401 accggcggta cccttcggtg cgccggcgcc acggccatga tgggcactcg agggggctca
     2461 cggcccctcg ccgcgggtgc tcgccagcag ggcccgagcg tcggccagcc acagccgcgg
     2521 caacgtgagg tgatccatcg ggtgcttcgc ggcgaggtcg gtgacggcgc gctcgaacgc
     2581 cgtgatcgcc tcgtcacgcc gcccgagcgc cagcaaggcg cgcccgcggg tcagctcgag
     2641 ctccgccaga tccgccgcga tccacggccg gtcgcggtac gccgcgaagg tggcgatgct
     2701 ggcatcgacg gcggcgaccg ccgctgcgtg gtcgcccgcg agcgaggcca ccagcgcggc
     2761 ggctccgtgc gcctgcaact gaccgtccgc gtcgtccggc tggccctcca cgcagcgtcg
     2821 cgtcgccacc agcgcggcgc cggcctgcgc gacatccccg agtcgcagct cgaggtggcc
     2881 aagcgtgtag ttgcacaggc tgcagctgtc ggcgtcctcg gggatgcgct cgcgcagcgc
     2941 cgggcaggtc gcgcgcagca ggtcgcgcgc ggccgccagg tccgggccga ggcgggcgcg
     3001 catcagcctc tgccgcagca ccaccgggtg ctgcgacccc agcagctgcg tcagcccagc
     3061 ctcgctgcgt gcgaataaca ggtcgcggcg ctgcgggtct tcggtcgtgt gcgcgaggct
     3121 cgcgaggtaa tatgcgcggt cgatcgggtc gacgtcgacg gcacggtccg actcggcgac
     3181 cgcgcgggcg aaggcctccg cggcgcgggc ccggtcgcca cgggccaagt gcgcggtgcc
     3241 ctcgttgctc gccacgcggg ccagcgccgc ggcagggtcc ggcaagcgct cggcgaagcc
     3301 acgcgccagc gggacctggg ccagcgccag ctcgacgcga ccggagtttt ggaccacctc
     3361 gacgaagatc gcgcgagaga agctctcggc ggcgatcgca tcctggcgcg tggcgaccgc
     3421 gatcgcggtc gcctcctcga gatgaccggc cgccgcggcc ccgcgcgcct ggttcatctc
     3481 gatcgtcgcc agggtcaaca gcgccccggc cagcgtcgga cgaaagccga cgcgccgcgc
     3541 ctcgagcacc gccgccgccg cgacgcgctc ggcaccctga agatcgcccg cgtggagctg
     3601 gttacgcgcg gagatcagcg cgccctcgac ctccgccacc gcggccgcga gcgccgggtc
     3661 gtccggcgcc gcgacctcgg ccatgacgac cgcgacgtcg ctgcacgacg cgatcgcagg
     3721 cagccgcgcg accagctggg ccgcgtccgg tggcgggccc gcgggcgccg tcagcagatc
     3781 gaccgcgctg gcgagcgcgc tccggcgttg ctgcaggcag cgcgtgcgcg cgtcgaacag
     3841 cgcgctcgac tgctcgtcgc ggcgatgcac gaggcaggcg tcgcggtgcg ccgcgaccca
     3901 gccgtccgcg taggcatcga ggccatcaac gacgcgcccc gcgagccctg gatcccgccc
     3961 ggtcgccagc gcctccgtga ccaccgcgtg gtccgcctcg tcccaccccg ccgcgatctc
     4021 gcgggctccg ccgctgcatg cattcgcctc gcgtgcgcgc cagtccgcga gcgccagccc
     4081 ggcccctccg gtcaagcctg cgaggccgac cgccgtcagc cagcgccagc gcgtccgcgg
     4141 cctgggggcc agcgcggcga gcagcgcgtg catgctcggg tagcgccccg ccggcgcgac
     4201 gacgagcccg cgacgcagca ccgcgatcag ccgccgcggg cctcgtgtgt ccgccggtgg
     4261 ctcggtgatc cggccggccg tgaccgcgag agcgatctgg gcgtgcgagt cgccgcgaaa
     4321 cgggcggccg ccgtacagcg cctcccacag cgccacgcag aagctgaact gatccgagcg
     4381 ggcgtccgcg acccggccct cgtgctgctc cggtgacatg tacgtcggcg tcccgagcag
     4441 cgccccggtc gccgtcagcg acatgtcgag cgggcgcgcc gcctggccgg cgtcgggtgc
     4501 acgcgcctcc tcggagccgt cgcgcgcgag cccgaagtcg gcgacgcaga cccggccgtc
     4561 ggcgccgacg agcacgttgt ccggcttgaa gtcgcgatgc acgagcccgg cgtcgtgggc
     4621 cgccgcgagc ccgcgaccgg cgtcgaggaa cacccgcagg atctcgtcga tgctgcgctc
     4681 ggccgcggtc aaccagccgt ggaggtcgac gccctggatg aactccatcg ctatgaacac
     4741 ctgctcgccg aacgcgtccg tgtcgtagat ctgcacgacg ttggggtgcg acagccgggc
     4801 cagcgcctgc gcctcacgca gcagacgcag ctgcgagtcg cgcccgccgt ggtccgtgcg
     4861 cagcagcttc accgcgaccc ggcggtcgag gtcggggtcg tacgcgctgt acaccacgcc
     4921 catgccgccg ctgccgatgc gcccgagcac gtgatagcga ccgacccgct gcggcgacgc
     4981 gcccgggtca tcctggcggg cgcgcccgcc tccggtcgcg gtgccggtgt cgtcgcgctc
     5041 cgacacgaag gcccgccccg cggagtcgga cgccagcgta tcggcgatca gtgtgtcagg
     5101 gaccgtcatg acgaccctca gttcgtatgc gtcgtccaga cgatccgcgg gtcctcgacg
     5161 tcgatcttgc tgcacggcgg caggacgcac tcgctctcga cgtcctggct gccgacgagg
     5221 cgcggcctgt cgaggcacag cgcgccgtcc gcggaccagt acgcctcgag cgcggcgttc
     5281 gcgggcggct cgatgccgac cgtcttgtcg gcgtccctcc agaggatcgg ggtaccctgc
     5341 gccgtgaagc taaagccctt gccgcagtaa tccgcggtga tcatcttgag cgtcgcctgc
     5401 cgctcgtcga cggtcgccgg cgagggcgtg ccattgaagt ccgcgttcgg accatagttc
     5461 atgcgctgca tcttgtacgc ggcctgatcg gcgcaggcga gcgtgaacca gccgtactgg
     5521 tccgggacca cgagcttgtg ctcgcggtcg taggtctcgt cgcggatcag ggtgacgacc
     5581 gtctcgtcgg gcgtggcctc ggggcacacg ttgaggatct cgccggggac caggtggctc
     5641 tcgtacttga gggcatatcg gaccatcggc ggggcgccgt ccgcgaggct ggggatcgtg
     5701 tcgaccccgg cgatctcgag caagtagtgg ccgtccgggc tgtccagatc gatcttccag
     5761 ccgagcaggt cggccccgct cagggtctgg ttcgggagca cgaccgcgaa ctgctcatcg
     5821 atcacgtgca gcgggtacga acccgtcgcg gagcggatcc tcagcagctg gacgccgtcc
     5881 gagttgagcg cgccgtcgag gtgaagctcg gggatctcga agtcgttgat ctcgggcgag
     5941 ttggggaagc ccaagccgca ggtcgtgcaa ctaccggtgg tacgaaactc gtggagcccc
     6001 gggtcgtccg cctcgtcggc gtaacaacca ccggccagga ccagcgggag gagagacaag
     6061 aggacgcggg gcgcggggag acggacgagc gagtggatgg gtgacatgga gagcctcatt
     6121 ttcacggtca cacagcagca agccccacgc catgacaaaa cccaagcaga tcaacgcggc
     6181 ggccggtccc gcgggcgctg gcccggaagt gtctccggag acacttgcgg ggactgtccc
     6241 cgcgacgacc atgccgacca tgccacaatg gcacgcggtc agcgaacggt gactcgatgt
     6301 cactatctcc gtcccccggc cccatcgcct ggccccgccc catcgcccca tcgcccggcg
     6361 cgaaactatc gtcttgccgc cgccgcggcc gcgggacaat gtgtttgccg atgacctcga
     6421 ggcgggccga cgacaccccc ggccggcgca gcgccgacga gaccgtctcg accgagcgct
     6481 cgaagcgaac ccacgccg
//
LOCUS       DASWNJ010001539         2858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_697039, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001539 DASWNJ010000000
VERSION     DASWNJ010001539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697039"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VGB85_25585"
     CDS             <1..605
                     /locus_tag="VGB85_25585"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0137492.1"
                     /translation="YGPEHPALWKPHRNLGSALLLAGDLPAARRHLERAIELVGASLG
                     PSHAATGPLQYALAASYADSDSDSESDRKKDLDHAFKLATIALQTHIREVPDNNPRRV
                     QPVILMGLLHTDRDEPAEALPYREEVLRRLRHAGPEDRADAHYNIAVTLHELHRSEEA
                     LDHIRLASALVSEFSTPSAELVEDIEAYKAEILRSLGRTQ"
     gene            620..1057
                     /locus_tag="VGB85_25590"
     CDS             620..1057
                     /locus_tag="VGB85_25590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137493.1"
                     /translation="MSQPTLIIHVVPIQVTLTGTTATITCNDPRAALVDDELVFTLNP
                     LDPNDGEAQPRVCFVVEDLPDDRGYDNYFTMSSAPPLEKLVAWKRTAEVSRSPEMLPG
                     HSFEVVAVAYSVPSSQPAAPVKLGHGRRRIKIYLTGAPGTVRY"
     gene            1071..>2858
                     /locus_tag="VGB85_25595"
     CDS             1071..>2858
                     /locus_tag="VGB85_25595"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEY0137494.1"
                     /translation="MPPASSARRALLISSPRNGLVATGPSLARMRTFLETQDFVIPDE
                     ACLDGKQATRDAIVSGFAALIDATRPGDVVVVYYAGHGGLFSDHVGATKQAHHVLEPM
                     DIADSDANHFNGLLGAELRLFLRALAHLCDNVTAIFDCCHASGLAVADAPADGLDEAR
                     DRENIAAIATRAGARIALRRAEEDAKNRLESTRSERDPLTTGVVRLVASSSSERAYAH
                     PRRPMMLFTDILVTVLEEHALADGLTWEQIVREVRARVQQIRPEQRPGVEGARHRRPF
                     TVDAAPSPIDHFHVQHDEGRLRLAAGEASGVRRDHRFELSPYAGGPALGTATAVRVHP
                     FHTFLEPPRSARPLPTVMFARRIREAPVELAARLVTSTDAADRAAAAELHARLAGQGH
                     HLRAPKIGDIAPGSVALVDELDPGVPDFVAQQVAHVALADPDAGDELVRAARRLERWA
                     GLAAQIGHPGLGPLTGCYSLEWGRLVGEQLEALGAGARVHVGEALRVCLYNPGRARTL
                     HIAAYRVRADRCIEVWRDIDGGLAVPTGHTSALDQVFDRVPGLAGAQHEWLVLAIGDG
                     DFDLGALTTPGGDVPRDPITRSFESSPGEA"
BASE COUNT          430 a         1090 c          911 g          427 t
ORIGIN      
        1 tctacggtcc cgaacatcct gcgctgtgga agccgcatcg caacctcggg agcgccctcc
       61 tcctggccgg tgacttgccc gctgcccgcc gtcacctcga gcgagccatc gagctcgtcg
      121 gggcgagcct cggaccgagc cacgccgcga ctggcccgct gcagtatgcg ctcgccgcct
      181 cttacgccga ctccgactcc gactccgaat ccgacagaaa gaaggacctc gaccacgcgt
      241 tcaagttggc gacgatagcg ttgcagactc atatccgcga ggtaccggac aataatccac
      301 gacgggtcca gccggtaatc ctgatgggac tacttcacac tgatcgggac gagcccgcag
      361 aggcactacc gtaccgtgag gaagtgctcc ggcgacttcg tcacgccggc cccgaagatc
      421 gggccgacgc ccactacaac atcgcagtga cgctgcatga gctgcaccgt tcggaagaag
      481 ctctcgacca tatccgcctc gcttctgccc tggtgagtga attttccacg ccatccgccg
      541 agctcgtcga ggacatcgag gcctacaagg ccgagatcct tcgctccctc gggcgtaccc
      601 aatgaccccg aaaagaacca tgtcccagcc caccctcatc atccacgtcg tccccatcca
      661 ggtcacgctc accggcacga ccgccaccat cacctgcaac gacccccgcg cggcgctcgt
      721 cgatgacgag ctcgtgttca ccctcaaccc cctcgacccg aacgatgggg aagcgcagcc
      781 gcgcgtctgc ttcgtcgtcg aggatctccc cgacgaccgg ggttacgaca attacttcac
      841 catgtcgtcg gcaccgccgc tcgagaagct ggtcgcctgg aagcgcaccg ccgaggtcag
      901 ccggtccccc gagatgctgc ccggccatag cttcgaggtc gtcgccgtcg cctatagcgt
      961 gcccagctcc caaccggcgg cgccggtcaa gctcggccac ggccgccggc gcatcaagat
     1021 ctacctgacg ggtgccccgg gtacggtccg gtactgacgc tcacgtcgcc atgcctcccg
     1081 catcctcggc gcggcgcgcc ctactgatca gcagcccgcg caacgggctg gtcgccaccg
     1141 gccccagcct cgcgcgcatg cgcaccttcc tcgagacgca ggacttcgtc atccccgacg
     1201 aggcctgcct cgacgggaag caggcgacgc gcgacgcgat cgtatccggc ttcgcggcgc
     1261 tgatcgacgc gaccaggccc ggtgatgtcg tcgtcgtcta ctacgcgggg cacgggggcc
     1321 tattctcgga ccacgtcggc gcgacgaagc aggcgcacca cgtgctcgag ccgatggaca
     1381 tcgccgactc ggacgccaac catttcaacg gattactcgg cgcggagctg cggctgttcc
     1441 tgcgggcact ggcgcacctc tgcgacaacg tcactgcgat cttcgactgc tgtcacgcct
     1501 ccgggctcgc cgtcgccgat gcgccggcgg atggcctcga cgaggcccgc gaccgcgaga
     1561 acatcgcggc gatcgccaca cgggccggtg cacgcatcgc gctgcgccgg gccgaggagg
     1621 acgccaaaaa tcgactcgag tcgacccgat cggagcgcga cccgctgacg accggcgtcg
     1681 tccgcctggt cgccagcagc tcgagcgagc gcgcgtacgc ccacccgagg cggcccatga
     1741 tgctgttcac cgacattctc gtgaccgtgc tcgaggagca cgcgctcgcc gatggcctga
     1801 cgtgggagca gatcgtccgc gaggtgcggg cgcgcgtgca gcagatccgg cccgagcagc
     1861 gcccgggcgt cgagggtgcg cgtcatcgcc ggcccttcac cgtcgacgcc gcgccgtcgc
     1921 cgatcgacca cttccacgtc cagcacgacg agggccgcct gcggctcgcc gccggtgagg
     1981 cctccggcgt gcgtcgcgac caccgcttcg agctctcgcc ctacgccggt gggcccgcgc
     2041 tcgggaccgc cactgccgtc cgggtccacc cctttcacac cttcctcgaa cctccgcgca
     2101 gcgcacgtcc gctgccgacc gtgatgttcg cgcgacggat ccgcgaggcg ccggtcgagc
     2161 tcgccgcgcg cctcgtcacc tccacggatg ctgccgaccg ggccgcggcc gccgagctgc
     2221 acgcgaggct cgcggggcag ggtcatcacc tccgcgcgcc gaagatcggc gacatcgcgc
     2281 ccgggagcgt cgccttggtg gacgagctcg accccggcgt ccccgacttc gtcgcgcagc
     2341 aggtcgcgca tgtcgccctc gcggacccgg acgccggtga cgagctcgta cgcgctgccc
     2401 gtcgcctcga gcgctgggcc ggcctcgccg cgcagatcgg tcacccgggg ctcggcccgc
     2461 tgaccggctg ttattcgctc gagtggggtc ggctcgtcgg cgagcagctc gaggcgctcg
     2521 gcgccggcgc gcgcgtccat gtcggcgagg cgctgcgggt ctgtctatat aatcccgggc
     2581 gggcgcgcac cctgcatatc gcggcgtacc gcgtccgtgc cgatcggtgc atcgaggtct
     2641 ggcgcgacat cgacggcggc ctcgccgtgc cgacgggcca cacctcggcg ctggatcagg
     2701 tcttcgaccg tgtgcccggc ctggcggggg cgcagcacga gtggctcgtg ctggcgatcg
     2761 gcgacggcga cttcgacctc ggcgcgctga ccactccggg cggcgatgtt ccccgcgacc
     2821 cgatcacgcg ctcgttcgag agctctcccg gcgaggcg
//
LOCUS       DASWNJ010001540         2380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_697047, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001540 DASWNJ010000000
VERSION     DASWNJ010001540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697047"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..>2380)
                     /locus_tag="VGB85_25600"
     CDS             complement(52..>2380)
                     /locus_tag="VGB85_25600"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein
                     kinase family protein"
                     /protein_id="HEY0137495.1"
                     /translation="ASTDRGAVPAFARGTDLADTAKDDARKEGKAGKAAAISAASAAA
                     ATSGSERPRLQSSQLETAIPGLSSVRLTRTGALVGTPAYMAPEQHIAARVDARSDQFA
                     FCIALYEALFGSHPFPAKNYVQLSLSVLGGKVDAMTGRNDVPVRVRRVILRGLSVDPD
                     HRFPNMDELLLALSVDPEQRRRRRVGLALLAGGVAGVMGLVGVEVFGGAPPCEGADRH
                     VAEVYNAGVRAEIEAAFMATGQPFAARVLESSLGGLDRWSARWGEQRREACELTHVHH
                     EQSTDLLDRRITCLDRHMRRLTATINVLRGANKDVVLHAVESVDALPDLAECEDNDRL
                     LAGEAPPPGQAALIAAAEKLLAEAEAARLSVQYDDAERLAGEALALATSEGLGRVEAQ
                     SLIMQGTIARNRSHFDRAEQIFTRAAALAEELGADELRALAWGELGMVLGLQRRIGEA
                     ERLLRHDERVLDRFGASRLERARLRERLGMVLAMDGRSEEAIDLLSASLADAEALLGE
                     RSSGLVSTLNARGTMHDQRGDTDAALADYARALAICEAHVGPDHPDIAGILGNMAILE
                     QNRKRYTPAREHFERALAIQRRSLGDGHPSTGWTHMNLGSLLLVSGVPAAAVEQFEKA
                     LVVQREAGVGDADLADLLYNLGLAHHMSGAYEQAFVSFSDALVRCERAHGSDDVAVAD
                     PLLGVGGALVELGRLDEARPLLERAHKLRTRDGATHADLGELRFALARALAPVDRDRA
                     LVLATQARSDYSGSGETDIVSTIDTWLKGQLKRRR"
BASE COUNT          300 a          883 c          874 g          323 t
ORIGIN      
        1 atcctcgcct gcgcacgcgc gtttattcgt tgcgcgcaca agtgcacgcg ctcaccggcg
       61 acgcttgagc tgtcccttga gccaggtatc gatcgtcgaa acgatgtccg tctcgccgct
      121 gccgctgtag tcgctgcgcg cctgggtggc cagcacgagc gcgcggtcgc ggtcgacggg
      181 cgcgagcgcc cgcgcgagcg cgaagcgcag ctcgccgaga tccgcgtgcg tcgccccgtc
      241 gcgggtccgc agcttgtggg ctcgctccag cagcggccgc gcctcgtcga ggcgaccgag
      301 ctcgaccagc gcgccgccga ccccgagcaa ggggtcggcg accgcgacgt cgtcggagcc
      361 gtgggcccgc tcgcagcgga ccagcgcgtc gctaaaggac acgaacgcct gctcgtacgc
      421 gccgctcatg tggtgggcga ggccgaggtt gtacagcagg tcggcgaggt cggcgtcccc
      481 gaccccggcc tcgcgctgga cgaccagcgc cttctcgaac tgctcgaccg ccgccgcggg
      541 aacaccgctg acaaggagca ggctgccgag gttcatatgc gtccagccgg ttgacgggtg
      601 gccgtcaccg aggctgcgcc gctggatcgc cagcgcccgc tcgaagtgct cgcgcgccgg
      661 cgtgtagcgc ttacggttct gctcgaggat cgccatgttg ccgaggatcc ccgcgatgtc
      721 cgggtggtcg ggcccgacgt gtgcctcgca gatcgcgagc gcccgcgcgt agtcggccag
      781 cgccgcgtcg gtgtcgccgc gctggtcgtg catcgtgccg cgcgcgttga gggtcgagac
      841 caggccggag ctgcgctccc ccagcagcgc ctccgcgtcg gcgagcgatg cggacagcag
      901 atcgatcgcc tcctcggagc gcccgtccat cgccagcacc atgccgagcc gctcgcgcag
      961 gcgggcccgc tcgagccgcg aggcgccgaa gcggtcgagg acccgctcgt cgtgtcgcag
     1021 caggcgctcg gcctcgccga tgcggcgctg caggccgagg accatgccga gctcgcccca
     1081 cgccaacgcg cgcagctcgt cggcgccgag ctcctcggcg agcgcggccg cccgcgtgaa
     1141 gatctgctcg gcgcgatcga agtggctccg gttcctggcg atcgtgccct gcatgatcag
     1201 gctctgcgcc tcgacccgcc cgagaccctc ggaggtcgcc agcgcgagcg cctcgccggc
     1261 cagtcgctcg gcgtcgtcgt actgcaccga gagccgcgcc gcctcggcct cggcgagcag
     1321 cttctcggcg gcggcgatca gcgcggcctg gcccggcggc ggggcctcgc ccgccagcag
     1381 ccggtcgttg tcctcgcact cggcgaggtc gggcagcgcg tcgaccgact cgaccgcgtg
     1441 cagcacgacg tccttgttcg cgccgcgcag cacgttgatc gtcgcggtca ggcgccgcat
     1501 gtgacgatcg aggcaggtga tccggcggtc gagcaggtcg gtcgattgct cgtggtgcac
     1561 gtgggtcagc tcgcaggcct cgcggcgctg ctcgccccag cgcgccgacc agcggtcgag
     1621 gccgccgagg gagctctcga gcacgcgggc ggcgaagggc tgcccggtcg ccatgaaggc
     1681 cgcctcgatc tccgcgcgca cgccggcgtt gtagacctcg gcgacgtggc gatcggcgcc
     1741 ctcgcacggc ggcgccccgc cgaacacctc gacgccgacg aggcccatca cgcccgcgac
     1801 gccgccggcg agcagcgcga ggccgacgcg acggcgacga cgctgctccg ggtcgacgct
     1861 gagcgcgagc agcagctcgt ccatgttcgg gaaccggtgg tcggggtcga ccgacaggcc
     1921 gcgcaggatc acccggcgca cgcgcaccgg gacgtcgttg cggccggtca tcgcgtcgac
     1981 cttgccgccg agcacgctca ggctcagctg cacgtagttc ttggccggga acgggtggct
     2041 gccgaacagc gcctcgtaca gcgcgatgca aaacgcgaac tggtccgagc gcgcgtcgac
     2101 gcgggccgcg atgtgctgct ccggggccat gtacgccggc gtaccgacca gcgcgccggt
     2161 gcgggtcagt cgcaccgagg acagccccgg gatcgccgtc tcgagctgcg aggattgcag
     2221 gcgcggacgc tcgctgcccg aggtcgccgc ggccgcgctg gcggccgaga tcgccgccgc
     2281 cttgccggcc ttgccctcct tgcgcgcgtc gtccttggcg gtgtcggcga ggtcggtgcc
     2341 acgcgcgaag gccggcacgg cgccgcggtc ggtcgaggcg
//
LOCUS       DASWNJ010001541         2305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_379115, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001541 DASWNJ010000000
VERSION     DASWNJ010001541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379115"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..942
                     /locus_tag="VGB85_25605"
     CDS             64..942
                     /locus_tag="VGB85_25605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY0137496.1"
                     /translation="MTPTASGRKVRPLESIAAHFVLILAVAFALYPILWVIALAFSGA
                     ADLEPRVLPLPIEPTLEHLRGVVGSTLKTPGEPERWLFGIQLLNSIVISLATAAVGIL
                     IAVPTAYALARFEFVGKDAGLRTLLATQMFPAVASAIPLYLILDALGLLGSRSGLVLC
                     YATTSVPFAIFQLRGAFETIPRDLEEAAMVDGATRFEAFIKVVLPAARPAIAVTGLFA
                     FMSAYNEFVLAHTMLGREELYTLPVVLQRYISEFSADWPKFAAGSLLVSLPVMLLFYV
                     AQRHLVAGLTAGGVKG"
     gene            983..>2305
                     /locus_tag="VGB85_25610"
     CDS             983..>2305
                     /locus_tag="VGB85_25610"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0137497.1"
                     /translation="MLITSRALPVLLPLTLLTACGDSGRADADGGGTGSTPATLSDPT
                     GATDPTTTGIHATMTGTGDGTGTSESVTGTQASDPTAGDTGTKFDLPGGNEGTATDPT
                     TDTDTGPPVDVCKVNDNMDAIGDCDEFAPPDSFAPSLQWSFGANEQSMVTPLAGNFTD
                     DNGDGAIDLCDIPDVLLVSGPTIGYGAICPIHVLDGATGTEHFQIPPSESVSCFATPA
                     FGDIDNDGLPEIVAVYNSGGTYRIKAFEHDGALKWANTTDGGGEQFSRESGAIALHDL
                     DADGDVEIIFNHEVYDHEGKLLWQQTNPMPGELEATTAADLDGDGKMEVITGHSAYRW
                     DGTVYFENYPTITSQSIPQVANLDDDPEPEIFITSGQGLWMVEHTGAIKWGPATPTGI
                     PANAYLVWQRPGTVHDFDGDGKPQWASSSQSFYAVYNGPTMADVQWQVT"
BASE COUNT          364 a          871 c          725 g          345 t
ORIGIN      
        1 cccgagacca ccgcccatca ccgcccgaac aatccctgat cttaacgacc cctgatcccc
       61 gccatgaccc ccactgcctc cggtcgcaag gtccgtccgc tcgagtcgat cgcggcgcac
      121 ttcgtgctga tcctcgccgt cgccttcgcg ctctacccga tcctgtgggt gatcgcgctc
      181 gccttctcgg gcgccgcgga cctcgagccg cgcgtgctgc ccctgccgat cgagccgacg
      241 ctcgagcacc tgcgcggcgt cgtcggcagc acgctcaaga cccccggcga gcccgagcgc
      301 tggctgttcg ggatccagct cctcaactcg atcgtcatct cgctggcgac cgccgccgtc
      361 gggatcctga tcgccgtgcc gaccgcctac gcgctcgcgc gcttcgaatt cgtcggcaag
      421 gacgccggcc tgcgcacctt gctggcgacg cagatgttcc ccgcggtcgc cagcgcgatc
      481 cccctgtacc tgatcctcga cgcgctcggc ctgctcggca gccggtccgg cctggtcctg
      541 tgctacgcga ccacctcggt gccgtttgcg atcttccagc tgcgcggcgc cttcgagacg
      601 atcccacgcg acctcgagga ggccgcgatg gtcgacggcg cgacccgctt cgaggcattt
      661 atcaaggtcg tcctgcccgc cgcgcgcccg gcgatcgcgg tgaccgggct gttcgcgttc
      721 atgagcgcct acaacgagtt cgtgctcgcg cacaccatgc tcggacgcga ggagctgtac
      781 accctgcccg tggtgctcca gcgctacatc tccgagttta gcgccgactg gccgaagttc
      841 gccgccggct cgctgctggt ctcgctgccg gtgatgctgc tgttctatgt cgcgcagcgc
      901 cacctcgtcg ccgggctgac ggccggcggc gtcaagggct agctcgcttg aattgccggc
      961 gcaggtcggc tgtagtggcg ccatgctcat tacgtcccgc gcgcttccgg tcctcctccc
     1021 tctcacgctg ctcactgcat gtggtgacag cggccgcgcc gacgccgacg gcggtggcac
     1081 cggcagcacg cccgcgaccc tgagcgaccc gaccggcgcg accgacccga cgaccaccgg
     1141 catccacgcg acgatgaccg gcaccggcga tggcaccggc acgagcgagt cggtgaccgg
     1201 cacgcaggcc tccgacccga ccgccggcga caccggcacc aagttcgacc tcccgggcgg
     1261 caacgagggc accgcgaccg acccgacgac cgacaccgat accggtccgc cggtcgatgt
     1321 ttgcaaggtc aacgacaaca tggacgcgat cggcgactgc gacgagttcg cgccgcccga
     1381 cagcttcgcg ccctcgctgc agtggagctt cggggccaac gagcagagca tggtgacgcc
     1441 gctcgccggc aacttcaccg acgacaacgg cgacggcgcg atcgacctgt gcgacatccc
     1501 ggatgtcctg ctcgtgtcgg gcccgacgat cggctacggg gccatctgcc cgatccacgt
     1561 gctcgacggc gcgaccggca cggagcactt ccagatcccg ccgagcgaga gcgtgtcctg
     1621 cttcgccacc ccggccttcg gcgacatcga caacgacggc ctgccggaga tcgtcgccgt
     1681 gtacaacagc ggcggtacct atcgcatcaa ggccttcgag cacgacggcg cgctcaagtg
     1741 ggccaacacg accgacggcg ggggcgagca gttctcgcgc gagagcggag cgatcgccct
     1801 gcacgacctc gacgccgacg gcgacgtcga gatcatcttc aaccacgagg tctacgacca
     1861 cgagggcaag ctgctgtggc agcagaccaa cccgatgccc ggcgagctcg aggcgacgac
     1921 cgcggccgac ctcgacggcg acggcaagat ggaggtgatc accggccact cggcgtaccg
     1981 ctgggacggc accgtctact tcgagaacta tccgacgatc acgagccagt cgatcccgca
     2041 ggtcgccaac ctcgacgacg accccgagcc cgagatcttc atcacctccg ggcagggcct
     2101 gtggatggtc gagcacaccg gcgcgatcaa gtggggtccg gcgacgccga ccggcatccc
     2161 cgcgaacgcc tacctcgtgt ggcagcggcc gggcaccgtg cacgacttcg acggcgacgg
     2221 caagccgcag tgggcctcct cctcgcagtc gttctacgcg gtctacaacg ggccgacgat
     2281 ggccgatgta cagtggcagg tgact
//
LOCUS       DASWNJ010001542         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_339036, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001542 DASWNJ010000000
VERSION     DASWNJ010001542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339036"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1627)
                     /locus_tag="VGB85_25615"
     CDS             complement(<1..1627)
                     /locus_tag="VGB85_25615"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEY0137498.1"
                     /translation="MRSSWAWVLLLAGGCFSGAGELTAGDGSSSGTSGTTGAGESTGD
                     TPTTGGATGPGPGGTQATEATGDTGTPDSSESSSSSGEPPPEPTPGLRGEYYGTYIDP
                     VIDRVDPVLDFDWADGAPGEGVGADRFSIRWTGQLVPPSSGPHTIITESDDGVRVWIG
                     DALVIDDWNGHFIKRNEAVVELVADVPVGLRIEYFELDLGSAARLKWSSPTLAEEVIP
                     QARLIAAEAPSGLPPPKPPFVNPVEAFDCPDPGVFGFEMADGLNFYKVCTGGSFPIRH
                     SRDLVLWNDTGAVVLPNGKPGWAANGNRNWAPEMHRVGDEFVVYFTTVNGVNVLSIGA
                     SHAPDPLGPYKGTGGPLVEHPLGVIDATYYNSGGTPYLIYKIDGNSQGKPTPIIARQL
                     APDGLSFAEGSGEVTLLVNQGDTWEGGVVEAQWIVENGGFHYMFYSGNVYDHRYRTGV
                     ARSVGLLGPYEKLGPPILGNNERWVGPGHGSVLAVEGIDYFIYHAWNNAGNGTHDAPK
                     GRNVLVDRIVWDNGWPRIHDGTPSRTQQQWPGVP"
     gene            complement(1655..>2542)
                     /gene="lysA"
                     /locus_tag="VGB85_25620"
     CDS             complement(1655..>2542)
                     /gene="lysA"
                     /locus_tag="VGB85_25620"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEY0137499.1"
                     /translation="AEELDRVAARARALGTVAAVALRVNPDIDPDTHPYLATGLRESK
                     FGVAMAEAEALASHATTLPSLRLIGMACHIGSQIADAVPFMAAFERLRGLVLRLRERG
                     VQLRQLDLGGGLGIAYTRDDPTLDVAAFGTAVTAAARDLGLELVLEPGRFLIGNAGVL
                     LTRVIGRKRGESRNFAIVDAAMNDLIRPALYGAYHPIVPVEVAAAGRAALRADVVGPV
                     CESGDFLGTDRLLPWPEPGELLAVLGAGAYGMTMASNYNTRALPAEVLVREDRFAVTR
                     PRRSIEALIAEERTPDWLE"
BASE COUNT          350 a          924 c          888 g          380 t
ORIGIN      
        1 agggcacgcc tggccactgc tgctgcgtgc ggctcggtgt gccgtcgtgg atccgcggcc
       61 agccgttgtc ccagacgatg cggtcgacga gcacgttacg acccttgggg gcgtcgtgcg
      121 tgccgtttcc ggcgttgttc cacgcgtgat agatgaagta gtcgatgccc tcgaccgcga
      181 gcacggaccc atggccgggc ccgacccagc gctcgttgtt gccgaggatc ggcggaccga
      241 gcttctcgta cggcccgagc aggccgaccg agcgcgcgac gccggtgcga tagcggtggt
      301 cgtacacgtt gccgctgtag aacatgtagt ggaagccgcc gttttcgacg atccactgcg
      361 cctcgacgac cccgccctcc cacgtgtcgc cctggttcac aagcagcgtc acctcaccgc
      421 tgccctcggc gaacgacagc ccgtccggcg cgagctggcg cgcgatgatc ggcgtcggct
      481 tgccctgcga gttgccgtcg atcttgtaga tcaggtaggg cgtgccgccg ctgttgtagt
      541 aggtcgcgtc gatgaccccg agcgggtgct cgacgagcgg cccgccggtg cccttgtacg
      601 gcccgagcgg gtccggggcg tgcgacgctc cgatgctgag cacgttgacc ccattgacgg
      661 tcgtgaagta gacgacgaac tcgtcgccga cgcggtgcat ctcgggcgcc cagttgcggt
      721 tgccgttggc cgcccagccc ggcttgccgt tcggcagcac gaccgcgccg gtgtcgttcc
      781 acagcacgag gtcgcgcgag tgccggatcg ggaacgaacc gccggtgcac actttataaa
      841 agttgaggcc gtcggccatc tcgaagccga acaccccggg atcggggcag tcgaaggcct
      901 cgactgggtt gacgaacggc ggcttgggcg gcggcaggcc gctcggcgcc tccgcggcga
      961 tcaggcgcgc ctgcgggatg acctcctcgg cgagcgtcgg gctcgaccac ttgagccgtg
     1021 ccgcgctgcc gaggtcgagc tcgaagtact cgatccgcag gccgaccggc acatcggcga
     1081 cgagctcgac caccgcctcg ttgcgtttga taaaatggcc gttccagtcg tcgatgacca
     1141 gcgcgtcgcc gatccacacg cgcacgccgt cgtcgctctc ggtgatgatc gtgtgcggtc
     1201 cgctgctggg cggcacgagc tgcccggtcc agcggatcga gaaacgatcg gcgccgacgc
     1261 cctcgcccgg cgccccgtcg gcccagtcga agtcgagcac gggatcgacg cggtcgatca
     1321 ccggatcgat atacgtcccg taatactcgc cacgcaggcc cggcgtcggc tccggcggcg
     1381 gctcgccgct gctgctgctg ctctcactgc tgtccggcgt gccggtgtcg cccgtggctt
     1441 ctgtggcttg cgtgccgccg gggcccggac ccgtcgcccc gccggtcgtc ggcgtgtcgc
     1501 ccgtgctctc gcccgcgccg gtggtccccg aggtcccact gctgctgccg tcgcccgcgg
     1561 tcagctcacc ggcgccgctg aagcagccgc cggcgagcag gagcacccac gcccatgaag
     1621 atcgcattcc ctgcgaggat acagcagccg cgcgctactc gagccagtcc ggcgtgcgct
     1681 cctcggcgat cagggcctcg atcgaccgcc ggggtcgcgt gaccgcgaat cgatcttcgc
     1741 gcaccagcac ctcggccggc agcgcccgcg tgttgtagtt cgaggccatc gtcatgccgt
     1801 acgcgcccgc gccgagcacc gccagcagct cgccgggctc gggccagggc agcaggcggt
     1861 cggtgccgag gaagtccccc gactcgcaca ccggtccgac gacatcggcc cgcagcgcgg
     1921 cgcggccggc ggcggcgacc tcgaccggca cgatcgggtg ataggcgccg tacagcgccg
     1981 gccggatcag gtcgttcatc gccgcgtcga cgatcgcgaa attacgcgac tcgccgcgct
     2041 tgcggccgat cacccgcgtc agcagcacgc ccgcgttgcc gatgaggaag cgcccgggct
     2101 cgagcaccag ctcgagcccg aggtcccgcg ccgcagccgt gaccgccgtg ccgaaggcgg
     2161 cgacgtcgag ggtcggatcg tcgcgcgtgt aggcgatgcc gaggccgcct ccgaggtcga
     2221 gctggcgcag ctgcacgccg cgctcgcgca gccgcagcac caggccgcgc aggcgctcga
     2281 acgccgccat gaagggcacc gcgtcggcga tctgcgagcc gatgtgacag gccatgccga
     2341 tcagccgcag cgacggcagc gtcgtcgcgt ggctcgccag cgcctcggcc tcggccatcg
     2401 ccaccccgaa cttgctctcg cgcaggccgg tcgccagata gggatgcgtg tcggggtcga
     2461 tgtcggggtt gacgcgcagc gccaccgcgg ccaccgtgcc gagcgcacgc gcgcgcgcgg
     2521 ccacgcggtc gagctcctcg gc
//
LOCUS       DASWNJ010001543         1549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_486828, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001543 DASWNJ010000000
VERSION     DASWNJ010001543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486828"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..>1549)
                     /locus_tag="VGB85_25625"
     CDS             complement(123..>1549)
                     /locus_tag="VGB85_25625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137500.1"
                     /translation="WRRQALDWSAGHRVLNDLTAMVVLPTASDYQRFGLEADDLRSLL
                     AVGTRGFEWRRNTMPADPTGSEPAAPRPYALRPERLAEKLLWDEPAAAAATTSPTAAV
                     RRDSQQRHAARRGRVQPGRVQPDRMQAGREDMSVAPRETEVPDKDAGAPQLGPRRTPD
                     DAYEGNFLTVMNLLAAWDAKHNALEVATRWRRAQPTDVMALVALGEALEANDRPEQAA
                     RAYGSIVDLYPGRADMRRLAAARLERLGELNTWLVLDSYGRALEERNEDPAGHRLYAF
                     ALLRAGYYQESFDALVDGLAWARSEPKTRPLEPVLLADLGIIAAAWIRRWPEQEAYVR
                     EALRVHEAELATTPSLRFVLSWDSELADLDLHVRDGLGWHAFYRSKVLASGGSLSDDM
                     DAGYGLEAFTIDGAATAFPYRLEVGYYARGATQGTGKIEVVEHDGNGQLFFDQRPFVV
                     NKQRAYVDLGMISKPPSQAPSKRR"
BASE COUNT          202 a          507 c          626 g          214 t
ORIGIN      
        1 ccagcttcgg gtgctctcgc gccgacaatg cttgaacacg tcatacgagg ctgctccgag
       61 agcgacgatg ctggcccacg cctgcgaggc accacgacgc ccgagcacgc agggagcccg
      121 agctagcgcc gcttcgaggg cgcctgcgag ggcggcttgg agatcatgcc gaggtcgaca
      181 taagcccgct gtttattgac gacgaagggc ctctgatcga agaacagctg gccgttgccg
      241 tcgtgttcga cgacttcgat cttgccggtg ccctgggtcg cgccgcgggc gtagtagccg
      301 acctcgaggc gatacgggaa ggccgtggcg gcgccgtcga tggtgaaggc ctcgagcccg
      361 tagccggcgt ccatgtcgtc ggagagcgat ccgccggagg cgaggacctt gctgcggtag
      421 aaggcgtgcc agccgaggcc gtcgcggacg tgcaggtcga ggtcggcgag ctcgctgtcc
      481 cagctgagca cgaagcgcag cgagggtgtg gtcgccagct cggcctcgtg gacgcgcagg
      541 gcctcgcgca cgtaggcctc ctgctcgggc cagcgacgga tccacgcggc cgcgatgatg
      601 ccgaggtcgg cgagcagcac cggctcgagc gggcgggtct tgggctcgga gcgggcccag
      661 gcgaggccgt cgaccagcgc gtcgaaggac tcctggtagt aaccggcgcg cagcagggcg
      721 aaggcgtaga ggcggtggcc ggccgggtcc tcgttgcgct cctcgagcgc gcggccgtag
      781 ctgtcgagca ccagccaggt gttgagctcg ccgaggcgct cgaggcgggc ggcggcgagg
      841 cggcgcatgt cggcgcggcc ggggtagagg tcgacgatcg acccgtacgc gcgggcggcc
      901 tgctcggggc ggtcgttggc ctcgagcgcc tcgccgagag cgacgagcgc catgacatcc
      961 gtcggctgag cgcggcgcca gcgggtcgcc acctcgagcg cgttgtgctt ggcgtcccac
     1021 gcggcgagca ggttcatcac cgtcaggaag ttgccctcgt aggcgtcgtc cggggtgcgg
     1081 cgcgggccga gctgcggggc gccggcgtcc ttgtcgggga cctccgtctc gcgtggcgcg
     1141 acggacatgt cctcgcggcc agcttgcatg cggtcaggtt gcacgcggcc aggttgcacg
     1201 cggccgcggc gggccgcgtg gcgctgctgc gagtcgcggc gcaccgcggc ggtcggcgag
     1261 gtcgtcgcgg cggcggccgc cggctcgtcc cacagcagct tctcggcgag gcgctcgggg
     1321 cggagcgcgt acggccgcgg ggccgccggc tcactgccgg tcgggtcggc gggcatcgtg
     1381 ttgcggcgcc actcgaagcc gcgggtgccg accgcgagca gcgagcgcag gtcgtcggcc
     1441 tcgaggccga agcgctggta gtcggaggcg gtcggcagca cgaccatcgc ggtgaggtcg
     1501 ttgagcacgc ggtggccggc ggaccagtcg agggcctggc ggcgccagc
//
LOCUS       DASWNJ010001544         6408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_486842, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001544 DASWNJ010000000
VERSION     DASWNJ010001544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6408
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486842"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VGB85_25630"
     CDS             <1..568
                     /locus_tag="VGB85_25630"
                     /inference="COORDINATES: protein motif:HMM:NF021220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HEY0137501.1"
                     /translation="AFERILDGAGDLPEGTADPGVPGLTELIDRLPAYLRPGPGEPDD
                     ARAPAEFVPWLARWVAVTLRDEWDDETRRRVVAEAMQLYRLRGTREGLRRMIGVYVGL
                     LDAVTIHEFTEVPHFFQVEVVLPSRDPKALARTDKSVRAIIDQEKPAHTVYGLRFAFP
                     SMQIVDHPTPARPGILLGVNTLLGSKFH"
     gene            587..2833
                     /locus_tag="VGB85_25635"
     CDS             587..2833
                     /locus_tag="VGB85_25635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137502.1"
                     /translation="MANETTTYTLYPEVSTDERGRYFDGQFLTAQDFVDEQRYHVDRL
                     RRALDHLTVAGVAEGLEVVGAGPWRVTVRRGAAIDPSGRLLVLATALENVDVPKDIPG
                     GVVEVALRYNEVESRVQGGTSDEDGTRGASRLRELPALDFYAPGAPPRPGVPLAQIKV
                     TESGAVSLVDPDPVRRHSGLRLPSSGAAGPTLRSGAPARPDLVGLTGDLRVSGRLGVG
                     TDDPEAELDIRGVARVGHLSVREHAFTLGGDETSFYPIVFRDLDWSAGAAVLEIVRVS
                     AQADNAGALIARIRWHAGDGQGAELLEAEVIQSRRFIANIKILKKDRLLAVWLRGGRT
                     YTWRSPSRIEIADDKLTSKNLGGEQLDFRATIEAVLDRDRVRLGVTFDREEFRGSLTV
                     TGDLAYTAYLNKLDVAENAEAVVRAYDFKIGHSTRRGSPGRALVDHAGALNVNFAGDW
                     PITRLHGNTEATGSLTVTGDTFLKKSVVITNNLDIDGEANFKKSLAITGNLGVGGDLS
                     ITGLAVLKKGVTISGALTLGGELQIDGAIVSKAGARLGDNLVISRNQEHVTLSRPKSS
                     EGGARLFLELVQLDGTTPETYAAIRFHHNNRYWHRLEARSNGLHVRDGDLGRDNYKNF
                     YAGEIRASGDLYANGLHVTTGRAERLRIIRGTVTLSGGIAEGSGFTVSRGDGWLTKIT
                     FNTAFSSTPTVVATQQYPDNNTSSEGGRTLDNAVIVRVEASAVWVKCGDSDGDGAWRR
                     FHFIAVGP"
     gene            2836..4965
                     /locus_tag="VGB85_25640"
     CDS             2836..4965
                     /locus_tag="VGB85_25640"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0137503.1"
                     /translation="MTAPLDSDAEHLAAELANLDLLLLREVVRARRAAGHAADERGLY
                     VSDDELQQILQRPFASPLWHDVPIDGALAELDALVRHDRSELDARITATLALGRTLRL
                     ERLVELHRDATPLPSFLKTLLLLALAPELDLRYERIFGWLHDDITRRHPSVQLALDLL
                     CPGPTARLAALHLFAADGPLRRLALLHTDAREPGAPLLRHELRLAPGLTTWLSGQPSI
                     PPALAPVVTRRAPDPALRAALHLSPELASAWDRLHAAALRGELALNVHGPDGAGRRAF
                     ATALCGALDRPLLIVDGARSEALDDAAFAGLLAVLARAVRLERAAICWTAFDRQRPAL
                     AALQGADLLFLVTHRPWHAAHPAGLHALAELHVPIAELPVRELLWQDALPDVPAGSLR
                     IAADGFRFTPTQIAAAAVSARGVAGAQGLAAPDLPAVLLACRRHATPKLGALAGAVTT
                     PLRWEDLILPDEHREQLREIVRRLHHRTRVLDDWGFGLGGARGLLALFAGAPGTGKTM
                     AAAIVARECARDLYRIDLSQVVSKYIGETEKHLELLFAEAEATDAALFFDEADAIFGK
                     RSPVKDANDRFANIEVGFLLQRIESFPGLVILATNLRKNIDAAFLRRLQVVVEFPTPD
                     LASRARIWAGVWPAGAPVDPSVDPQLLAERFDLSGGHIRNIALSAAYAAAEANSPITL
                     RHVLTAARGEYRKLDKLTREHSFTEPT"
     gene            complement(4970..5401)
                     /locus_tag="VGB85_25645"
     CDS             complement(4970..5401)
                     /locus_tag="VGB85_25645"
                     /inference="COORDINATES: protein motif:HMM:NF039577.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137504.1"
                     /translation="MGTPLLLTGDLAVFPTAFGAAVVPAPLTAPMVGSGTLTVDGKPV
                     CVAGDEAKVVVACTYTAGAFSVPGAGMLTIAALASDQPSQVVTTPGKAVLLARSKFTA
                     QLQVTTPAQAPPGTVPATDVMVLYTGEGEFQSSNTRVNAKE"
     gene            complement(5406..>6408)
                     /locus_tag="VGB85_25650"
     CDS             complement(5406..>6408)
                     /locus_tag="VGB85_25650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137505.1"
                     /translation="RRQASFPGEVIISGDEADPHPDEFLAEARREAPGVLFSVSHGEG
                     APRGGWRSAAEQRARQGAMSFGRAGGHLEAEAIRGASFMPGGLWFMLACFGAGTPERS
                     KYKRWLDALLAEGQFAGRPEVVLGSLPAPGVAPFIAALPKAALASPRGPLAFVGHVDL
                     AWTYSFRELDSGKAQNRPTRFVQTLASVLRGDRVGVSFHELFRYFEQANTELTTLEDA
                     EVDAPRRRAHLWMLRNDLAGYILLGDPAARLQLGPPEPPPVARTLQEEATSLFGFGVM
                     PDVPAGPALSVIESAIGALLAGAALAEVATRHAIGESELREWFEKYRAAGRAAIGRG"
BASE COUNT          908 a         2652 c         1981 g          867 t
ORIGIN      
        1 cgccttcgag cggatcctcg acggcgccgg cgacctgccc gaagggacag ccgaccccgg
       61 cgtgcccggc ctgaccgagc tgatcgaccg cctgccggcc tacctgcgcc ccggccccgg
      121 cgagcccgac gacgcccgcg ccccggccga gttcgtgccg tggctcgcgc gctgggtcgc
      181 cgtcaccctg cgcgacgagt gggacgacga gacccgccgc cgcgtcgtcg ccgaggcgat
      241 gcagctgtat cgcctgcgcg gcacccggga gggcctgcgc aggatgatcg gcgtgtatgt
      301 cggtttgctc gacgccgtca cgatccacga gttcaccgag gtcccgcact tcttccaggt
      361 cgaggtcgtg ctgccgagcc gcgaccccaa ggcgctcgcg cgcaccgaca agtcagtgcg
      421 cgcgatcatc gatcaggaga agcccgcgca caccgtttac ggcctgcgct tcgccttccc
      481 ctcgatgcag atcgtcgacc acccgacccc cgcgcggccc ggcatcctgc tcggcgtcaa
      541 caccctgctc ggcagcaagt tccactagca cccgcgagcc tccatcatgg ccaacgaaac
      601 caccacctac acgctctacc ccgaggtctc gaccgacgag cgtggccgct acttcgacgg
      661 ccagttcctg accgcgcagg acttcgtcga cgagcagcgc taccacgtcg accgcctgcg
      721 ccgcgccctc gaccacctga ccgtcgccgg cgtcgccgag ggcctcgagg tcgtcggcgc
      781 cggaccgtgg cgcgtcaccg tccgccgcgg cgccgcgatc gacccctccg gccgcttgct
      841 cgtgctggcg accgcgctcg agaacgtgga tgtcccaaag gacatccccg gcggcgtcgt
      901 cgaggtcgcc ctgcgctaca acgaggtcga gtcgcgcgtg cagggcggaa cctccgacga
      961 ggacggcacc cgcggcgcca gccgcctgcg cgagctgccc gcgctcgact tctacgcccc
     1021 cggcgccccg ccccgccccg gcgtcccgct cgcgcagatc aaagtcaccg agtccggcgc
     1081 tgtcagcctc gtcgaccccg accccgtgcg ccgccacagc ggcctgcgcc tgccgagcag
     1141 cggcgccgcc ggcccgaccc tgcgcagcgg cgccccggcg cgccccgacc tcgtcggcct
     1201 gaccggcgac ctgcgcgtca gcggccggct cggcgtcggc accgacgacc ccgaggccga
     1261 gctcgacatc cgcggcgtcg cccgcgtcgg ccacctctcc gtgcgcgagc acgcgttcac
     1321 cctcggcggc gacgagacga gcttttaccc gatcgtgttc cgcgacctcg actggagcgc
     1381 cggcgcggcc gtgctcgaga tcgttcgcgt cagcgcccag gccgacaacg ccggcgcgct
     1441 gatcgcccgg atccgctggc acgccggcga cggtcagggc gccgagctgc tcgaggccga
     1501 ggtcatccag agccgccgct tcatcgccaa catcaaaatt ttaaaaaagg accgcctgct
     1561 cgccgtgtgg ctgcgtggcg gccggaccta tacctggcgc tctccgtcgc gcatcgagat
     1621 cgccgacgac aagctgacca gcaagaacct cggcggcgag cagctcgact tccgcgccac
     1681 gatcgaggcc gtgctcgacc gcgaccgcgt gcgcctcggc gtcaccttcg accgcgaaga
     1741 attccgcggc tcactgaccg tcaccggcga cctcgcttac accgcgtacc tcaacaagct
     1801 cgacgtcgcc gagaacgccg aggccgtcgt ccgcgcgtac gacttcaaga tcggccactc
     1861 gacgcgccgc ggctcgcctg gccgcgcgct cgtcgaccac gccggcgcgc tcaacgtcaa
     1921 cttcgcaggc gactggccga tcacccggct gcacggcaac accgaggcga ccgggagcct
     1981 gaccgtcacc ggagacacct tcctcaagaa gagcgtcgtc atcaccaaca acctcgacat
     2041 cgacggcgag gccaatttca agaagagcct ggcgatcacc ggcaacctcg gcgtcggcgg
     2101 cgacctctcg atcaccggcc tggccgtgtt gaagaagggc gtcacgatct ccggcgccct
     2161 caccctcggc ggcgagctgc agatcgacgg cgcgatcgtc tcgaaggccg gcgccaggct
     2221 cggcgataat ctcgtgatct cccgcaacca ggagcacgtc acgctgtctc gaccgaagag
     2281 cagcgagggc ggcgcgcggc tgttcctcga gctggtccag ctcgacggca ccacgcccga
     2341 gacctacgcg gcgatccgct tccatcataa caatcgctac tggcatcgcc tcgaggcccg
     2401 ctcgaatggc ctccacgtcc gagacggcga cctcggccgc gacaactaca aaaatttcta
     2461 tgccggtgag atccgagcct ccggcgacct ttacgccaac ggcctgcacg tcaccaccgg
     2521 ccgcgccgaa cgcctgcgca tcatccgggg caccgtcacc ctcagcggag ggatcgccga
     2581 gggctcgggc ttcaccgtgt cgcgtggcga cggctggctg accaagatca ccttcaatac
     2641 cgccttctcc agcacgccca ccgtcgtcgc cacccagcag tacccggaca acaacaccag
     2701 ctccgagggc ggcaggaccc tcgataacgc ggtcatcgtc agggtcgagg cgtccgccgt
     2761 gtgggtgaag tgcggcgaca gcgacggcga cggtgcctgg cgccgcttcc acttcatcgc
     2821 cgtgggccca tgagcatgac ggctcccctc gacagcgacg ccgagcacct cgccgccgag
     2881 ctcgccaacc tcgacctgct gctgctgcgc gaggtcgtgc gggcccgtcg tgctgcgggc
     2941 cacgccgccg acgagcgcgg cctctacgtc tccgacgacg agctgcagca gatcctccag
     3001 cgcccgttcg ccagcccgct gtggcacgac gtcccgatcg acggcgccct cgccgagctc
     3061 gacgcgctcg tgcgtcacga ccgcagcgag ctcgacgccc gcatcaccgc caccctcgcc
     3121 ctcggccgca cgttgcgcct cgagcggctc gtcgagctcc accgcgacgc cacaccgctc
     3181 ccctcttttt taaaaaccct gctgttgctc gccctcgcac ccgagctcga cctccgctac
     3241 gagcgcatct tcgggtggtt gcacgacgac atcacgcgcc gccaccccag cgttcagctc
     3301 gcgctcgacc tgctctgccc gggcccgacc gcccgcctcg ccgcgctgca cctcttcgcc
     3361 gccgacggcc ccctgcgccg cctcgccctg ctgcacaccg acgcgcgcga gcccggcgcc
     3421 ccgctgctgc gccacgagct gcgcctcgcg cccggcctga ccacctggct ctcgggacag
     3481 ccgtcgatcc ctccggcgct cgcgcccgtc gtcacccggc gcgcgcccga cccggccctg
     3541 cgcgccgccc tgcacctgtc ccccgagctc gcgtccgcct gggatcgcct gcacgccgcc
     3601 gccctgcgcg gcgagctcgc gctcaacgtc cacggccccg acggcgccgg tcgccgcgcc
     3661 ttcgccaccg ccctgtgcgg cgcgctcgac cgtccgcttt tgatcgtcga cggcgcgcgc
     3721 agcgaggcgc tcgacgacgc cgccttcgcc ggcttgctcg ccgtgctcgc ccgcgccgtc
     3781 cgcctcgagc gcgccgcgat ctgctggacc gccttcgatc gccagcgccc cgccctcgcc
     3841 gccttgcagg gcgccgacct gctgttcctc gtcacccacc ggccatggca cgcggcccac
     3901 cccgccggcc tgcacgcgct cgccgagctg cacgtcccga tcgccgagct gccggtccgc
     3961 gagctgctgt ggcaagatgc cctgcccgac gtccccgcgg gatctctgcg catcgccgcc
     4021 gacggctttc gcttcacgcc gacgcagatc gcagccgctg ccgtctcggc gcgcggcgtc
     4081 gccggggcgc agggcctcgc cgcgcccgac ctgccggccg tgctgctcgc gtgccgccgc
     4141 cacgcgacgc ccaagctcgg cgcactcgct ggcgccgtca cgaccccgct gcgctgggag
     4201 gacctgatct tgcccgacga gcaccgcgaa caattgcggg agatcgtccg caggctgcat
     4261 catcgcaccc gcgtgctcga cgactggggc tttggcctcg gcggcgcgcg tggcctgctc
     4321 gcgctgttcg ccggcgcgcc cgggaccggc aagaccatgg ccgccgcgat cgtcgcccgc
     4381 gagtgcgcgc gcgacctcta ccgcatcgac ctctcgcagg tcgtcagcaa gtacatcggc
     4441 gagaccgaga agcacctcga gctgctgttc gccgaggccg aggcgaccga cgccgccctg
     4501 ttcttcgacg aggccgacgc gatcttcggt aaacgctccc ccgtcaagga cgccaacgac
     4561 cgcttcgcca acatcgaggt cggcttcctc ctccagcgca tcgagagctt ccccggcctc
     4621 gtcatcctcg ccacgaatct acgcaagaac atcgacgccg cgttcctgcg caggctgcag
     4681 gtcgtcgtcg agttcccgac ccccgacctc gccagccgcg cccgcatctg ggccggcgtg
     4741 tggccggccg gcgcccccgt cgacccctcc gtcgacccgc aactgctcgc cgagcgcttc
     4801 gacctcagcg gcggacacat ccgcaacatc gcgctcagcg ccgcctacgc cgccgccgag
     4861 gccaactcgc cgatcaccct gcgccacgtc ctcaccgccg cgcgcggcga gtaccgcaag
     4921 ctcgacaagc tgacccgcga gcacagtttt acagagccga cctgagccgt cactccttcg
     4981 cgttcacccg cgtattgctg ctctggaact cgccctcgcc ggtgtagagc accatcacgt
     5041 ccgtcgccgg caccgtaccc gggggcgcct gcgccggggt cgtcacctgc agctgcgcgg
     5101 tgaacttcga gcgcgccagc agcaccgcct tgcccggcgt cgtcacgacc tgtgacggtt
     5161 gatcgctcgc cagcgccgcg atcgtcagca tgccggcgcc cggcaccgag aacgccccgg
     5221 ccgtgtatgt gcacgcgacg acgaccttcg cctcgtcccc cgcgacacac accggcttgc
     5281 cgtcgaccgt cagcgtgccg ctgccgacca tcggcgcggt caatggcgcc ggcaccacgg
     5341 ccgccccgaa cgcggtcggg aacaccgcca gatctcccgt cagcagcagc ggcgtgccca
     5401 cgcgatcacc ccctcccgat cgccgcgcga ccggccgcgc ggtacttttc aaaccattcc
     5461 cgcagttcgc tctccccgat cgcgtgccgc gtcgcgacct ctgccagcgc cgcgccggcg
     5521 agcagcgcac cgatcgccga ctcgatcacc gacagcgccg gcccggccgg cacgtccggc
     5581 atgaccccga agccgaacag cgacgtcgcc tcctcctgca gcgtgcgcgc caccggaggc
     5641 ggctccggcg ggccgagctg cagccgcgcc gccgggtcgc cgagcaggat gtaaccggcg
     5701 aggtcgttgc gcagcatcca caggtgcgcc cgccggcgcg gcgcgtcgac ctcggcgtcc
     5761 tcgagcgtcg tcagctccgt gttggcctgc tcgaagtagc gaaacaattc atgaaacgac
     5821 acgccgaccc ggtccccgcg cagcaccgac gccagcgtct gcacgaagcg cgtcggccgg
     5881 ttctgcgcct tgccgctgtc gagctcgcga aagctgtacg tccacgcgag gtcgacgtgg
     5941 ccgacgaagg ccagcggccc gcgtgggctc gccagcgccg ctttcggcag ggccgcgatg
     6001 aacggcgcga ccccgggcgc cggcaggctg ccgagcacca cctccggccg ccccgcgaac
     6061 tgcccctccg cgagcaacgc gtcgagccag cgcttgtact tgctgcgctc cggcgtgccg
     6121 gcgccgaagc acgcgagcat gaaccacagc ccgccgggca tgaagctcgc cccgcggatc
     6181 gcctctgcct cgaggtgccc gcccgcgcgc ccgaagctca tcgccccctg tcgcgcgcgc
     6241 tgctccgcgg cggaccgcca cccgccgcgc ggcgccccct cgccgtggct gaccgagaac
     6301 agcacgccgg gcgcctcgcg gcgcgcctcc gcgagaaact cgtcgggatg cggatccgcc
     6361 tcgtcgccgc tgatgatcac ctcgcccggg aacgacgcct gccggcgc
//
LOCUS       DASWNJ010001545         3166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_339177, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001545 DASWNJ010000000
VERSION     DASWNJ010001545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339177"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VGB85_25655"
     CDS             <1..496
                     /locus_tag="VGB85_25655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137506.1"
                     /translation="FTCKDGGTVLLTLENNGSPDCEDGSDEHEPSMCLDGSDEPGCAT
                     FEDLLALLQSFGAYEPSCGGFFDPPCPESPDVVELVTPDWSEPICGGPYGPPCLEWPH
                     GVEFKKFDCMDGKTIPMFSACNGIVDCDNGSDEVGCLNPFNYDLMPSSGLKVHVCDTW
                     PDCP"
     gene            812..1423
                     /locus_tag="VGB85_25660"
     CDS             812..1423
                     /locus_tag="VGB85_25660"
                     /inference="COORDINATES: protein motif:HMM:NF016286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HEY0137507.1"
                     /translation="MKLIQTAIESLQAKGLCPRLHVNVTHEDVVLPDFVRDKWKERLV
                     IDLDPSYPLDLAFTEVGVEADLSFGGYVTRCTFPWAAIYVVADRGTGRGQVFAGNIPD
                     SLRHEYGAPAPQAPALDPELTKIHDDTKPKKGSRRHRRAGAESPVSPLQGVRPLAPVL
                     APVEDASEAEQTPAEAAVVEVVASGEQTAQKRRSAFKVIDGGN"
     gene            1999..>3166
                     /locus_tag="VGB85_25665"
     CDS             1999..>3166
                     /locus_tag="VGB85_25665"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEY0137508.1"
                     /translation="MFDDLLAPIETQREHDVMADIFGAAGVEVLKMDGLLQAALRSAP
                     KPAVQLLLKRVCEAAGATHAVDELADWPADRLAQGLIAGVFWAELACATGSLTRLHAE
                     LVNDRPMALRPVPNLMFMRDPCIAVYDRVVVARMATAARAREPFIVSFALRHGAASGP
                     IDLEFAEDRASRHAELHSLEGGDLLVLSRRALMIGCSDRTSPQTIERIAEDALFPDHP
                     SLESIYVVMMPHGRTVMHLDTILTQVDRQLFLAHAPLIAGRSDARPLPVARLSRGGKA
                     ELLRSATVLDVLREELGRDVELVPCGGRDPLYQEREQWTDGANAVALAPGHILLYARN
                     THTIAMLAEYGFGRTDLSAVLPREQRAALVREGLARERNVFTFTGSELSRARGGG"
BASE COUNT          492 a         1086 c         1079 g          509 t
ORIGIN      
        1 gttcacgtgc aaggatggcg gcacggtgct gctgacgctc gagaacaatg gatctcctga
       61 ttgcgaggac ggctccgatg agcacgagcc atccatgtgc ctggacgggt ccgacgaacc
      121 aggatgcgca accttcgagg atctcctggc gctcctgcag tcgttcggcg cgtacgagcc
      181 gagctgcggc ggcttcttcg acccaccgtg ccccgagtcg cccgacgtgg tcgagctcgt
      241 gactcccgac tggtccgagc cgatttgtgg cggcccctac ggcccaccgt gcctcgagtg
      301 gccccacggg gtcgagttca agaagttcga ctgcatggat ggcaagacga tcccgatgtt
      361 ttccgcgtgt aacggcatcg tcgactgcga caacggctcc gacgaggtcg gctgcctgaa
      421 tcctttcaac tacgatctca tgccctcgtc cggcctgaag gtgcacgtct gcgacacctg
      481 gcccgactgc ccgtgaagca gtcgagtccg gcatcggctt cgtgcagccc ctgctcgact
      541 cggacgaggg cggagcgtcc gcgatcgagg gcgcggggcg cgacaactcc ttgggcagcg
      601 cgagcgagcc gcgcagcgcg agcgcgagga ggcccacgag caccgcggtg catgcagcga
      661 cgggtcagga acgcggcgag atcatcacgc gcgcggccga cggcgctcat ccaggtggcc
      721 agcgctgtcg aggctgagcc cgagaccgcg cccgaatccg aggccgaggg ctgacgctcc
      781 agaagcttgt ttggtagggt cggcttcgct catgaagctg attcaaaccg ccatcgagtc
      841 gctccaagcg aagggcctgt gcccgcgctt gcacgtgaac gtgacccacg aagacgtggt
      901 cctgcccgac ttcgtccgcg acaagtggaa ggagcggctc gtcatcgatc tcgatcccag
      961 ctacccgctg gacctcgcgt tcaccgaggt cggcgtcgag gcggatctct cgttcggcgg
     1021 ctatgttacg cgttgcacgt tcccatgggc tgcgatctac gtcgtcgccg atcgcggcac
     1081 gggccgcggc caggtgttcg ccggcaacat ccccgactcg ctgcgtcacg agtacggagc
     1141 tcccgccccg caggccccgg ccctcgaccc cgagctgacc aagatccacg acgacaccaa
     1201 gcccaagaag ggctcgcgtc gccacaggcg tgccggggcc gagtcgcctg tcagcccgct
     1261 ccagggcgtg cgcccgctcg cgccggtcct cgcgccggtc gaggacgcga gcgaggccga
     1321 gcagacgccc gccgaggctg cggtcgtcga ggtcgtcgcg tccggcgagc agacggcaca
     1381 aaagcgccgg tccgcattca aggtgatcga cggcgggaac tgagcgccgc cgccgatgcg
     1441 ccgggcgggg tgcggtcgac ttcggcctct gtctcggtgc ccttcgcgcc tggccctcgg
     1501 accaggttga gcgtcgccgc gcaaacgcca aggggtagga tccgttcggc ttcgtagccg
     1561 gcagtggatg tcctttggga caggttattc gagcggcacg acgacaggtt cggcgggcga
     1621 gagctccgca agtggcggtg cgaccgtgac ggcaccttga aagacaaggc cgtagcgagg
     1681 ggccacagcc acgccatcgg gggcgtcaac gtgtcgcagg gggccgcgga ttcccgggtc
     1741 ccacctcggg aaccacggcc tgcggcggtg ctcgatcggt accgcggtcc cagtctcagg
     1801 cgacggttct ggacggtcgg gcggcgcccg cgttgtccgc gcagcggact cacgcttgtg
     1861 ccgagaccat agctcgtgca gcatccgcgg ttcatgccgg tcgccgttca tagtgaagtc
     1921 gggcggcttc gacgcgtgct gatccaccgg cccggggagg agatcgcgcg catgacgcaa
     1981 cacgacctcc agggcatgtt gttcgacgat ctgctcgcgc cgatcgagac gcagcgcgag
     2041 cacgatgtca tggccgacat cttcggcgcc gccggagtcg aggtgctgaa gatggatggg
     2101 ctgctgcagg cggccctgcg atcagcgccg aaaccagccg tgcagctgct cctcaagcgc
     2161 gtgtgcgagg cagcgggcgc tacacatgct gtggacgagc tcgcggactg gccggcggat
     2221 cgcctggcgc agggcctcat cgccggtgtc ttctgggccg agctcgcctg tgcgacgggc
     2281 agcttgacgc gcttgcacgc ggagctcgtc aacgatcgac cgatggcgtt gcgacccgtg
     2341 ccaaacctca tgttcatgcg cgacccgtgt atcgcggtct acgaccgcgt cgtggtcgcc
     2401 cgcatggcca cagccgcacg ggcgcgcgag ccgttcatcg tctccttcgc cctgcgccac
     2461 ggcgcagcga gtggcccgat cgacctcgag ttcgccgagg accgtgcgag tcgacacgcc
     2521 gaattgcact cgctcgaggg cggcgacctg ctcgtcctct cgcgtcgtgc gctgatgatc
     2581 ggctgctccg accgcacgtc gccgcagacg atcgaacgca tcgccgagga cgcgctcttt
     2641 cccgatcatc cgagcctcga gtcgatctac gtcgtcatga tgccgcatgg ccgcaccgtg
     2701 atgcatctcg acacgatcct cacgcaggtc gatcgccagc tattcctcgc ccatgcgccg
     2761 ctcatcgccg gtagaagcga cgccaggccg cttccagtcg ctcgcctctc ccgcgggggc
     2821 aaggcggagc tcttgcgttc agcgaccgtt ctggacgtgc tgcgcgagga gctgggccgc
     2881 gacgtcgagc tcgtcccgtg cggcggccgc gacccgctgt accaggagcg cgaacagtgg
     2941 acggacggtg ccaacgcagt cgcgctcgcg ccagggcaca tcctgttgta cgcgcgcaac
     3001 acccatacga tcgccatgct cgccgagtat gggttcgggc gcaccgacct ctcggccgtg
     3061 ctgccccggg agcagcgcgc ggcgctcgtg cgcgaaggcc tggctcgtga gcgtaacgtg
     3121 ttcacgttca ccggcagcga gctgtcgcgc gcacgcggcg gtggcc
//
LOCUS       DASWNJ010001546         2700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_205173, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001546 DASWNJ010000000
VERSION     DASWNJ010001546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205173"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VGB85_25670"
     CDS             complement(<1..466)
                     /locus_tag="VGB85_25670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137509.1"
                     /translation="MLTGDSPGMAAHAGGFVLGVFALLLLGSLLQRVRLLWHTQLRSA
                     LVRVGAALWAWTGRVWPRDEAEADKRPPMGAGDRIALVAGLLLCALDVALTTLLLRDV
                     FPEPPYRFELGGWLSPAAAEWSFYLVVALFKTLLELWFGVFDKIRSAVAGPSA"
     gene            complement(953..1972)
                     /locus_tag="VGB85_25675"
     CDS             complement(953..1972)
                     /locus_tag="VGB85_25675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137510.1"
                     /translation="MYRRILIASCLATAACTAKTSRLEELSERNEAAGLPAEAPVDHP
                     LPPAPTLSRHAVTLAATPAGIDDRLWREIGWFPADTTDIGSFTDSTSESLARSAADEW
                     GGKFAECRPVFASIERTYTVWQAGRVNATSVYYGKMTRAQERECLQAALGTFGATGGQ
                     RGDLTIVSTPDGAWPRLAWFGRDEGTVVIREGDVPLEEWIKPAGTLADLPALVRLVAA
                     VDRSQGMWSVGLRDYGTAFTGVESTGYMLVMATPATRGERFFMATGQLEFASPEAAKQ
                     AELGAAAFAREVPSAAELGIVLTVQAEGNWLKSEIKADTSKTDSATITAWLQQVLARS
                     RANGA"
     gene            2103..>2700
                     /locus_tag="VGB85_25680"
     CDS             2103..>2700
                     /locus_tag="VGB85_25680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137511.1"
                     /translation="MRRPASIVLALLSLGGMMGCPLPPEQVTCEEEDACETSTTSGAG
                     GSTGSSEPEGDTRDIDTGTSTGAGASMGSTGDESTGTTGPDETASSTDEGTTGMAPFC
                     GDGVVNQDFEECDDGSPNPDGPCSPLCQRERIIFVLSLKLNGKLSGLQGADAYCRSQA
                     SKAQEADPASPIKDAKNFKALLPSSTESIFERHFRGEGR"
BASE COUNT          438 a          958 c          927 g          377 t
ORIGIN      
        1 tcgcgctcgg cccggcgacc gcgctgcgga tcttgtcgaa cacgccgaac cacagctcga
       61 gcaatgtctt aaaaagtgcg acgacgaggt agaacgacca ctcggcggcc gcgggtgaca
      121 gccagccgcc gagctcgaag cgatacggcg gctcggggaa cacgtcgcgc agcagcaggg
      181 tcgtcagcgc gacgtcgagg gcacacagca gcaggccggc gacgagcgcg atgcggtcgc
      241 cggcgcccat cggcgggcgc ttgtcggcct cggcctcgtc gcgcggccac acgcggccgg
      301 tccaggccca cagggccgcg ccgacgcgca ccagcgccga gcgcagctgc gtgtgccaca
      361 gcagccgcac gcgctgcaac aggctgccga gcagcagcag ggcgaacacg ccgagcacga
      421 agccgcccgc gtgagcggcc atgccgggcg agtcgccggt gagcatggcc cgcgcgagca
      481 ctccgacggc ggcgagcccg gtggcgagga cgatcagtcg aacgcgtcgc ggcgagcgct
      541 gcggtcgcga cggggatgtt ggggcgagtg acacggcgga cctccgggag cgatcggccc
      601 cgcgacggca cctcgaagaa atggtgcacg cgcgctggtt tgcggtctgt cccgaaggac
      661 ctgtcctcgg ggccacacgc cggctgcgcg ctcgaccgga gtcgacgcgg ccgcgggtcc
      721 ggggcaagca cgcgtgaggg atacgtggtt attccgggcg gaggcatcga gtgtatccgt
      781 cgacccccga gatcgcgcgc tcatgggccg gccgaccagg cccacgtcgc cgccgggcca
      841 tgttcggaat tgttgcgctc gcccgcgccg agctcttgat cccgcttctg aagttgccgc
      901 ggagtcatgc ccgaagaccc cgagcgcggc gaacctcccg gctggaaacc gctcaagcgc
      961 cgttggcccg gctcctggcg agcacttgct gtaaccaggc cgtgatcgtc gcggagtcgg
     1021 tcttgctcgt gtccgccttg atctcgctct tgagccagtt gccctccgcc tgcacggtca
     1081 aaacgatgcc cagctcggcc gcgctgggca cctcgcgggc gaacgccgcg gcgccgagct
     1141 cggcctgctt tgcggcctcc ggcgacgcga actcgagctg acccgtcgcc atgaagaaac
     1201 gctctccacg ggtcgctggg gtcgccatca ccagcatata accggtgctc tcgactccgg
     1261 tgaaggccgt cccatagtcc cgcagtccga cgctccacat gccctggctg cggtcgaccg
     1321 cggcgaccag gcggaccagc gcgggcaggt ccgcgagcgt gcccgccggc tttatccact
     1381 cctccagcgg cacgtcgccc tcgcggatga ccacggtccc ctcgtcgcgg ccaaaccacg
     1441 cgagacgcgg ccaggcgccg tccggggtcg agacgatcgt caggtctcca cgctgcccgc
     1501 ccgtggcacc gaacgtcccc agcgcagcct gcagacattc gcgctcctgc gcccgcgtca
     1561 tcttgccgta atacacgctg gtcgcattga ccctacccgc ttgccacacc gtgtatgtgc
     1621 gctcgatgct cgcaaacacc ggacgacact cggcgaactt cccgccccac tcgtccgcgg
     1681 ccgagcgcgc cagcgattcc gacgtgctat cggtgaaact gccgatgtcg gtggtatcgg
     1741 ccggaaacca gccgatctct cgccagagcc gatcatcgat ccccgcgggc gtcgcagcga
     1801 gggtcacggc gtgccggctg agcgtcggcg ccggcggcaa cgggtgatcc accggcgcct
     1861 cggccggcaa acccgcggcc tcgttcctct cggagagctc ctcaagacgg ctcgtcttgg
     1921 ccgtacaggc cgcggtcgca agacatgacg cgatcaggat ccgccggtac atcagcactc
     1981 cccgctgcct cgggagcagc cggcggaccg tatcacgccc ggccgccgca gcactatcgg
     2041 tcaccggagg tatcgaggat atccgttgac acgcgaaatc gcgcgtgcat atcatggctg
     2101 cgatgcggcg ccctgcttcg atcgtgctcg ccctgctctc cctcggagga atgatggggt
     2161 gcccgctgcc acccgagcag gtgacgtgcg aggaggaaga tgcgtgcgag acgagtacga
     2221 cctccggcgc cggcgggagc acggggagct cggagccgga gggggatacc cgcgacatcg
     2281 acaccggtac gagcacgggt gctggcgcga gcatgggttc gaccggggac gagagcaccg
     2341 gcacgacggg cccggacgag accgcctcga gcacggacga ggggacgacc gggatggcgc
     2401 cgttttgcgg cgatggtgtc gtcaaccagg acttcgagga gtgcgacgac ggctcgccga
     2461 atccggacgg gccgtgctcc ccgctgtgcc agcgggagag gattattttt gtcctcagcc
     2521 tcaagctcaa cggcaagctg agcggtcttc agggcgcgga cgcgtactgt cgctcgcagg
     2581 cgtccaaggc ccaggaggcg gaccccgcct cgccgatcaa ggacgccaag aacttcaagg
     2641 cgctgctgcc gagctcgacc gagtcgatct tcgagcgcca cttccgcggc gagggccgct
//
LOCUS       DASWNJ010001547         3686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_311006, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001547 DASWNJ010000000
VERSION     DASWNJ010001547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311006"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1773
                     /gene="tssI"
                     /locus_tag="VGB85_25685"
     CDS             <1..1773
                     /gene="tssI"
                     /locus_tag="VGB85_25685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HEY0137512.1"
                     /translation="QYRETDWDFVRRLLAEDGLYHYFEHTSSGHKLVIGDGTAAYGTI
                     GGDAIVGFNDLEGAGDRDVVQRFRLTQQVTPGKFTVRDFNFATPDDTLEGQASAGAGD
                     LEVYEFPGRYDAPALARRAAKLRLQAFQALQLRGEGESTCPRLLPGHTFELSSSTGAL
                     RDDVQRRYLVLSVRHHGGQPQVLGVSGAGQDSRYGNSFECIPADVVYRPAEWPLRPEM
                     RGVQTAIVVGPAGEEVHTDEHGRVKVHFHWDRMGRRDENSSCWVRVSQMWAGQGYGSM
                     WIPRIGHEVIVDFIEGDADRPIITGRVYHGNNPPPYPLPDKKTASTIKSDSSPGGGGS
                     NELRFEDQKGGEEVFLHAQKDWTIAVENDKNQTVGHDETMNVGNDRTKSVGNNQSESI
                     GVDKSIAVGSNHSEQIGANMSISIGANLEEQVGANATFTVGANRSETVAIASDESIGA
                     AKTLTIGAAYQVSVGGVMNVSVGLAKAEEVGLARSVVVGADSSLSVGGGHSVNAGANV
                     STSAGANIMISAAQNMNLSANKDLRVSVGKRAGVVAGDKLTIEVGSATVTISKNGDIS
                     ISGKKLSIKATGEVVIKGSKIKLN"
     gene            1818..3062
                     /locus_tag="VGB85_25690"
     CDS             1818..3062
                     /locus_tag="VGB85_25690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02270 family protein"
                     /protein_id="HEY0137513.1"
                     /translation="MSESVFEPLWDIVEQHLDEAEFLWEQWEASLVAPNYALREVEEG
                     PEARLIAHLDGLVVNGPLVVERLLLPTIADVDAEPTRVRAAALALLQTPGPEGFEAVF
                     AELHSSPPQRQELARALECSDLGPLRPRLELMLSAEEADLQYTAARILAFHGEALDVL
                     VPRMLASRRAVDRMLGLRTIPRLADAARHARAIVSALGSDDPDVRDAALTAGVLLDLP
                     NAWRCARELVAGVDPGAGHALLLLALRGDPSDHPAVLAAAPSLGAPGLWALGFLGTVE
                     AIDTVMPLLEDEVHARLAGEVVAAITGIDLEDENFTRVPPEEESLSHRPEDDLPLPDA
                     LKLLMWWRKQRPRFAVGERYMGGQPRDAATLLAALHEGPMRRRAAHLLALQLELPASR
                     RPHFEPMAPTFRQRRDLAKMPV"
     gene            complement(3077..>3686)
                     /locus_tag="VGB85_25695"
     CDS             complement(3077..>3686)
                     /locus_tag="VGB85_25695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137514.1"
                     /translation="GPVFTAIPTSPRPVPADRTEQYERSDVDSTADATQALPRIPATT
                     KPSRPAAASLAAPLSAIDDESTQALPVSVRRPAGPGAAPDPAERTEIDMRRRGIPDMP
                     RDAPAIGKQPAAFDDEMTIIGPPLRVALPPVPEATMLLPKTGEHPIARMDPDASVDPD
                     RQSPAVSTDPTTHRSSAQLQRILIAINICVALVLIAIAWATL"
BASE COUNT          599 a         1210 c         1343 g          534 t
ORIGIN      
        1 cagtatcgcg agacagactg ggactttgtc cgccgcttgc tggcagagga cggattgtac
       61 cactatttcg agcacacatc ctcgggccac aagctggtga tcggcgacgg cacggccgcc
      121 tacgggacga tcggcggcga cgcgatcgtc gggttcaacg atctcgaggg cgcgggcgac
      181 cgcgacgtcg tccagcgctt tcgcctgacg cagcaggtga cccccggcaa attcaccgtc
      241 cgcgacttca acttcgcgac ccccgacgac accctggagg gccaggcgag cgccggcgcg
      301 ggcgacctcg aggtgtatga attccccggt cgctacgacg ccccggcgct ggcccggcgg
      361 gccgccaagc tgcgcctgca ggcgtttcag gcgctgcagc tgcgcggcga gggcgagagc
      421 acctgcccac ggctgctccc ggggcacacc ttcgagctca gctcgagcac cggagcgctg
      481 cgagacgacg tgcaacgcag gtatctggtg ctctccgtgc gccaccacgg cggacaaccg
      541 caggtgctgg gggtcagcgg agcggggcag gactcgcgct acggcaattc gttcgagtgc
      601 attccggccg acgtcgtcta tcgtcccgcc gagtggccgc tgcgccccga gatgcggggc
      661 gtgcagacgg cgatcgtggt cggcccagcc ggcgaggagg tacacaccga cgagcacggg
      721 cgggtcaagg tgcactttca ctgggaccgc atgggcaggc gcgacgagaa cagctcgtgc
      781 tgggtccgcg tcagccagat gtgggccggc cagggttacg gctcgatgtg gatcccgcgc
      841 atcggccacg aggtgatcgt cgatttcatc gagggtgacg ccgaccgccc gatcatcacg
      901 ggccgcgtgt atcacggcaa caacccgccg ccctatccgc tgccggacaa gaagaccgcg
      961 agcacgatca agtcggactc gagcccgggc gggggcgggt cgaacgagct gcgcttcgag
     1021 gaccaaaagg gcggcgagga ggtgttcctc cacgcgcaaa aggactggac gatcgccgtc
     1081 gagaacgaca aaaaccagac ggtcggccac gacgagacga tgaacgtcgg gaacgatcgc
     1141 acaaagtcgg tcggcaacaa tcaaagcgag tcgatcgggg tcgacaagtc gatcgccgtc
     1201 ggctccaacc acagcgagca gatcggcgcc aacatgtcga tctcgatcgg cgccaacctc
     1261 gaggagcagg tcggcgcaaa tgcgacgttc acggtcggcg ccaaccgcag cgagacggtg
     1321 gcgatcgcca gcgacgagtc gatcggcgcg gccaagacgc tcacgatcgg cgcagcctat
     1381 caggtcagcg tcggcggggt gatgaacgtc tcggtcgggc tggcgaaggc cgaggaggtc
     1441 gggctcgcca ggagcgtcgt cgtcggggcc gacagcagcc tcagcgtcgg cggcggccac
     1501 agcgtgaacg ccggcgccaa cgtcagcacg tcggccggcg cgaacatcat gatctccgcg
     1561 gcccagaaca tgaacctgtc cgccaacaag gacctgcggg tcagcgtcgg caagcgggcc
     1621 ggcgtggtcg ccggggacaa gctgacgatc gaggtcggca gcgcgacggt gacgatcagc
     1681 aagaatggcg atatttcgat cagcgggaag aagctcagca tcaaggccac cggcgaggtc
     1741 gtcatcaagg ggtcgaagat caagctcaac tagaccccgc ggcgattttt atgcagggcg
     1801 attgaaacaa aggtcgtatg tccgagagcg tgttcgagcc gttgtgggat atcgtcgagc
     1861 agcacctcga cgaggccgag ttcctgtggg aacaatggga ggccagcctg gtcgccccga
     1921 attatgcgct gcgcgaggtc gaggagggcc ccgaggcgag gttgatcgcc cacctcgacg
     1981 ggctcgtcgt caacggccca ttggtggtcg agcggctgct gctgccgacg atcgccgatg
     2041 tcgacgccga gccgacgagg gtccgcgcgg ccgcgctggc gctcctgcag acgccgggcc
     2101 cggagggctt cgaggcggtg ttcgccgaat tgcattcgtc accgccgcag cggcaggagc
     2161 tggcgcgcgc gctcgagtgc agcgacctcg ggccgctgcg cccgcggctc gagctgatgc
     2221 tgtcggccga ggaggcggac ttgcaatata ccgcggcccg gatcctcgcg tttcacggcg
     2281 aggcgctcga cgtgctggta ccacgaatgc tggcgagccg ccgcgcggtc gaccgcatgc
     2341 tcgggcttcg cacgatcccg cggctcgccg acgctgcacg ccacgccagg gcgatcgtca
     2401 gcgcgctggg ctcggatgac ccggacgtgc gtgacgcggc cctgacggcc ggcgtgctgc
     2461 tcgacctccc gaacgcgtgg cgctgcgcgc gagagctcgt cgctggcgtc gaccccggtg
     2521 cagggcacgc gctgctcctg ctcgcgctgc gcggggaccc gtcggaccac ccggcagtcc
     2581 tggccgccgc accctcgctc ggcgcgccgg gtctgtgggc gctcgggttc ctcggtaccg
     2641 tcgaggcgat cgataccgtg atgccgctgc tcgaggacga ggtgcacgcg cgtctcgcgg
     2701 gcgaggtggt cgctgcgatc acggggatcg atctggaaga cgagaacttc acgcgcgtcc
     2761 cgccggagga ggagagcctc tcgcaccgcc cggaggacga cctgccgctg cccgacgcgc
     2821 tgaagctgtt gatgtggtgg cgcaagcagc gtccgcgatt cgcggtcggt gagcgctaca
     2881 tgggcggcca gccgcgcgac gcggcgacgc tgctcgcggc gctgcacgag ggcccgatgc
     2941 ggcggcgagc cgcgcatctg ctggccctac agctcgagct gccggcgagc cgtcgcccgc
     3001 acttcgagcc gatggcgccg accttccgac agcgccggga cctcgccaag atgccggtgt
     3061 gaccccccgc cgatcgtcac agagtggccc aggcgatcgc gatcagcacg agcgccacgc
     3121 agatattgat cgcgatcaag atccgctgca gctgtgcgga tgaccggtgg gtcgtggggt
     3181 cggtcgatac tgccggcgac tggcggtcgg gatcgacgct cgcgtcgggg tccatgcgag
     3241 cgatcgggtg ctcgccggtc ttcggcaaca gcatcgtggc ctcggggacc gggggcagag
     3301 cgacgcgcag cggcggcccg atgatcgtca tttcgtcgtc gaacgctgcc ggctgcttgc
     3361 cgatcgccgg cgcgtcccgt ggcatgtcag ggatcccgcg acgacgcata tcgatctcgg
     3421 tgcgctccgc gggatcgggg gcggctccag gcccggccgg acggcggacg ctcaccggta
     3481 gcgcctgcgt cgattcgtcg tcgatggcgg agaggggcgc cgcgaggctc gccgccgcgg
     3541 gtctgctcgg ctttgtcgtc gcggggatcc gtggcagcgc ctgcgtcgcg tcggccgtgc
     3601 tgtcgacgtc ggagcgctcg tactgttcgg tgcggtcggc cggcacaggt cgaggagagg
     3661 tgggtatcgc tgtgaacacc gggccg
//
LOCUS       DASWNJ010001548         2118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_520710, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001548 DASWNJ010000000
VERSION     DASWNJ010001548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520710"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1211
                     /locus_tag="VGB85_25700"
     CDS             <1..1211
                     /locus_tag="VGB85_25700"
                     /inference="COORDINATES: protein motif:HMM:NF045283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion IcmF C-terminal
                     domain-containing protein"
                     /protein_id="HEY0137515.1"
                     /translation="TRGPTMPLRKAFQKLQENVVLKDDFDYGEGIQLPFAQKKPGTTS
                     SMRAGDIARNFAPLLAFTVPPTGKEADVALDNYHVHVREVRDAIGKALDNKEEEKALL
                     ERLKGAIEDTKSLINDGNLEAWAKDTEELLLTPLKELLKLVERTGSEGAKNDWCARIV
                     DPMYERFNGRYPFAADARDDVVLADFEEFFHPENGVIRKAREELLSGFVALEGNYFVA
                     RDLGKSDVARLDPEVIRFLNRAQDIGMVMFVNEELRVDYDVFLECNPQVNRIEWKVAG
                     ETRVFECNNKQLQRMRWPAKEGQGAALTAFGRQGRKTLEEAGEWGLFELLERYSKIPA
                     FANEDVLEFKFDLTAFNLGTLDVRLKPTRVRGGTAFFGLPNGSRDYLSLLRSASVIPP
                     KRLFSNRGGC"
     gene            1213..>2118
                     /gene="tssC"
                     /locus_tag="VGB85_25705"
     CDS             1213..>2118
                     /gene="tssC"
                     /locus_tag="VGB85_25705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HEY0137516.1"
                     /translation="MSASVSVLDGLLEGVPASEHPAIKRGLARALQAALAAGGRRALE
                     SVDAVFVDTLLVEIDRRVSAQLDELLHHPDIQRLEAAWRGLALVVDQVRFEENIQIEL
                     VNVSKQALADDFADAPEIPKSGLYRLAYTGAYATFGGKPYGLICADYEFGPGAEDLGL
                     LRQCAAVAAMAHAPFITNIGPDFFGLKSFAELPRVRDLPAILAGPRYRMWHAFRETED
                     ARYVGLCLPRFLLRAPYRPDTDPFQPLRFRETVDEHHDHHLWGHASFAFAARAADSFA
                     RYRWCVYIIGSRASGTVEGMVRHPFP"
BASE COUNT          335 a          777 c          706 g          300 t
ORIGIN      
        1 tcacgcgcgg gccgacgatg ccgctgcgca aggcgttcca gaagctgcaa gagaacgtcg
       61 tgctcaagga cgacttcgac tacggcgagg gcatccagct gccgttcgcg cagaagaagc
      121 ccggcacgac cagctcgatg cgcgcggggg acatcgcccg caacttcgcg ccgctgctgg
      181 ccttcaccgt gccgccgacc ggcaaggagg ccgacgtcgc gctcgacaac taccacgtgc
      241 acgtgcgtga ggtccgcgac gcgatcggca aggcgctcga caacaaggag gaggagaagg
      301 cgctgctcga gcggctcaag ggcgcgatcg aggacaccaa gtcgctgatc aacgacggca
      361 acctcgaggc gtgggccaag gacaccgagg agctgctgct gacgccgctc aaggagctgc
      421 tcaagctcgt cgagcggacc ggcagcgagg gcgccaagaa cgactggtgc gcgcgcatcg
      481 tcgacccgat gtacgagcgc ttcaacggcc gctacccgtt cgccgccgac gcgcgcgacg
      541 acgtcgtgct cgccgacttc gaggagttct tccacccgga gaacggcgtg atccgcaagg
      601 cccgcgagga gctgctcagc ggcttcgtcg cgctcgaggg caactacttc gtcgcccgcg
      661 acctcggcaa gtcggacgtc gcccgcctcg accccgaggt gatccgcttc ctcaaccgcg
      721 cccaggacat cggcatggtg atgttcgtca acgaggagct gcgcgtcgac tacgacgtgt
      781 tcctcgagtg caacccgcag gtcaaccgca tcgagtggaa ggtcgccggc gagacgcgcg
      841 tgttcgagtg caacaacaag cagctgcagc gcatgcgctg gcccgccaag gaggggcagg
      901 gcgccgcgct gacggcgttc ggccggcagg gccgcaagac cctcgaggag gccggcgagt
      961 ggggcctgtt cgagctgctc gagcgctaca gcaagatccc cgcgttcgcc aacgaggacg
     1021 tgctcgagtt caagttcgac ctcaccgcgt tcaacctcgg caccctcgac gtgcgcctca
     1081 agccgacccg cgtgcgcggc ggcacggcct tcttcggcct gcccaacggc agccgcgact
     1141 acctgagcct gctgcgctcc gcgagcgtca tcccgcccaa gcggctgttt agcaaccggg
     1201 gagggtgctg acatgagcgc gagcgtcagc gtgctcgacg gcctgctcga gggcgtgccc
     1261 gcctccgagc acccggcgat caagcgcggc ctggcccgcg cgttgcaggc cgccctcgcc
     1321 gccggcggtc gccgggccct cgagtcggtc gacgccgtgt tcgtcgacac cctgctcgtc
     1381 gagatcgacc gccgcgtgtc ggcgcagctc gacgagctgc tgcatcaccc ggacatccag
     1441 cggctcgagg cggcctggcg cggcctggcg ctcgtcgtcg accaggtgcg cttcgaggag
     1501 aacatccaga tcgagctcgt caacgtcagc aagcaagcgc tcgccgacga cttcgccgac
     1561 gccccggaga tccccaagtc gggcctgtat cgcctcgcgt acaccggcgc ctacgcgacg
     1621 tttggaggca agccgtacgg cctgatctgc gccgactacg agttcgggcc gggcgccgag
     1681 gacctcggcc tgctgcgcca gtgcgccgcg gtcgcggcga tggcccacgc gccgttcatc
     1741 accaacatcg gccccgactt cttcggcctg aagtcgttcg ccgagctgcc gcgcgtgcgc
     1801 gacctgccgg cgatcctcgc cgggccgcgc taccgcatgt ggcacgcgtt ccgcgagacc
     1861 gaggacgccc gctacgtcgg cctgtgcctg ccgcggttcc tgctgcgcgc gccctaccgg
     1921 cccgacaccg acccgttcca gccgctgcgc tttcgcgaga cggtcgacga gcaccacgac
     1981 caccacctgt ggggccacgc ctcgttcgcc ttcgcggcgc gcgcggccga ctcgttcgcc
     2041 cgctaccgct ggtgcgtgta catcatcggc tcgcgcgcca gcggcaccgt cgagggcatg
     2101 gtccgccacc cgttcccg
//
LOCUS       DASWNJ010001549         3781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_559368, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001549 DASWNJ010000000
VERSION     DASWNJ010001549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559368"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VGB85_25710"
     CDS             <1..642
                     /locus_tag="VGB85_25710"
                     /inference="COORDINATES: protein motif:HMM:NF023413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEY0137517.1"
                     /translation="IQDFLQRKKTPTLKIVMRADVQGSAEALKQALLDLRTEKVKVEV
                     VLAGVGAITQTDVKMASAGEAVIIGFNAKPVGKAAQTAESEKVQIFVFNVIYDALDKV
                     RELMLGLLEPEYREREQGEAEVRALFPIPKIGVVAGCRVTRGLIQRSSHVRVVRRGVI
                     VHKGRIGSLRVMKDDVKEVRDGFECGIVIDTFPEIEPGDIIQAFDMEMLTPTL"
     gene            745..1149
                     /gene="rbfA"
                     /locus_tag="VGB85_25715"
     CDS             745..1149
                     /gene="rbfA"
                     /locus_tag="VGB85_25715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEY0137518.1"
                     /translation="MTTRMERVEETLRRAIADILLVGGLRDPRLQGVNFGVTGVKVDA
                     DLTLARVFIDVLSQGADESAVLAGLRAAAAAVRHELRGKVQMRRLPQLRFERDPSIQS
                     GLQVERILAEIRAGEASRVEPVAEPEGSEKKD"
     gene            1153..2235
                     /gene="truB"
                     /locus_tag="VGB85_25720"
     CDS             1153..2235
                     /gene="truB"
                     /locus_tag="VGB85_25720"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY0137519.1"
                     /translation="MAATAAGSAPWGVLLVDKPVGPTSHDVVGWVRWAIGEQQVGHCG
                     TLDPAASGLLVICVGSATRLVPYLTGVDKIYRARFILGRSTTTEDREGATLEQAAVTP
                     EIHAAARHALAGLHGALMLPPPAFSAVKIAGRRAHELARRGDVVELPPRPMTVLDVSD
                     LEADRDDAIEPWLDATMTVSKGTYIRALAVELGRRAGIPAHLGALRRLASGSLALDDP
                     RTVRDIVCEPLPDPLPGLPPKLRLRWPAADSPRDATREALRAALIDPCDALDLPRVAI
                     AADDPRMSALTRLRCGQSVAVDAAFADLPAGRILGGEGLAPAARLVLHDAAGLVIVRR
                     EVTEDGERLRPERVLAPATPAGAARE"
     gene            2378..2647
                     /gene="rpsO"
                     /locus_tag="VGB85_25725"
     CDS             2378..2647
                     /gene="rpsO"
                     /locus_tag="VGB85_25725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY0137520.1"
                     /translation="MSLSVDRKAEVVTKFQTHEGDTGSPEVQVALLTTRINYLTEHFR
                     THAKDFHSRRGLLKLVGQRRRLLDYLRGTSVDRYRSLIGALNIRK"
     gene            2873..>3781
                     /locus_tag="VGB85_25730"
     CDS             2873..>3781
                     /locus_tag="VGB85_25730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY0137521.1"
                     /translation="MNVITESCSIGEIEISIETGKLAKQANSVVIRCGETSVLVTAVS
                     QKDPKDLPFLPLTVEYREYNAAAGKIPGGYFKREGRLTDQETLTCRFIDRPIRPLFPK
                     SYRNDTQIIGTVLSHDKVHEPDVLAITGASAALMISDAPWAGPIAGVRVARVQGKFVA
                     FPSWDQIKVADIVVIVAVSRDAIVMVEGGADQASESDMIDALMFAKEAAAPLIALQDR
                     LRERAGKPKRQHVEPTVDLDFRGQVIAAAEAGLSAAFAITGKAERRAAIDKAVKAVSE
                     KFLADAPTRKAEVESAISKLEKKIVRS"
BASE COUNT          588 a         1414 c         1234 g          545 t
ORIGIN      
        1 atccaggact tcttgcagcg caagaagacg ccgaccctca agatcgtcat gcgggcggac
       61 gtgcagggct ccgccgaggc gctcaagcag gcgctgctcg acctcaggac cgagaaggtc
      121 aaggtcgagg tcgtgctcgc cggcgtcggc gcgatcaccc agaccgacgt gaagatggcc
      181 tccgcgggcg aggccgtgat catcggcttc aacgccaagc cggtcggcaa ggccgcgcag
      241 acggcggagt cggagaaggt ccagatcttc gtcttcaacg tcatctacga cgcgctcgac
      301 aaggtccgcg agctcatgct cggcctgctc gagccggagt accgcgagcg cgagcagggc
      361 gaggcggagg tccgcgcgct gttcccgatc ccgaagatcg gcgtcgtcgc cggttgccgc
      421 gtcacccgcg gcctcatcca gcgcagcagc cacgtccgcg tggtgcgtcg cggggtgatc
      481 gtccacaagg gccgcatcgg cagccttcgt gtcatgaagg atgacgtcaa ggaagtccgc
      541 gacggcttcg agtgcggcat cgtgatcgac accttcccgg agatcgagcc cggcgacatc
      601 atccaggcct tcgacatgga gatgctgacc ccgacgctgt aaggcgcggg gcgggcaggg
      661 gacgggcgtt ccgggacctc cggggcgccc atttccattt atgtctcatc atctttttgc
      721 gcatttcgag aggagcgagc cggcatgacc acacgcatgg aacgagtcga ggagaccctc
      781 cgccgggcga tcgccgacat cctgctcgtc ggcggcctgc gggacccgcg gctgcagggc
      841 gtcaacttcg gcgtcaccgg cgtcaaggtc gacgccgatc tgaccctggc ccgcgtgttc
      901 atcgacgtgc tgtcgcaagg cgccgacgaa tctgccgtgc tcgccggcct acgcgcggcg
      961 gctgccgccg tgcgccacga gctgcggggc aaggtgcaga tgcgacgact cccgcaactc
     1021 cgcttcgagc gcgacccgag catccagagc ggtctgcaag tcgagcggat cctcgcggag
     1081 atccgcgccg gggaggcgag ccgggtcgag ccggtcgccg agcccgaggg ctccgagaag
     1141 aaggactagg cgatggccgc gacggccgcc ggatccgcgc cgtggggcgt cttgctggtc
     1201 gacaagccgg tcgggccgac ctcgcacgac gtcgtcggct gggtgcgctg ggcgatcggt
     1261 gagcagcagg tcggccactg cggcaccctc gatccagcgg cctcgggcct gctcgtgatc
     1321 tgcgtcggct ccgcgacgcg cctggtcccg tacctgaccg gcgtcgacaa gatctaccgc
     1381 gcccgcttca tcctcggccg cagcaccacc accgaggacc gcgagggcgc cacgctcgag
     1441 caagccgcgg tcaccccgga gatccacgcg gccgcccgcc atgcgctcgc cgggctgcac
     1501 ggcgcactga tgttgccgcc gcccgccttc tcggcggtca agatcgccgg ccgtcgcgcc
     1561 cacgagctcg cccgccgcgg cgacgtcgtc gagttgccgc cgcgaccgat gaccgtgctc
     1621 gacgttagcg acctcgaagc cgaccgcgac gacgcgatcg aaccctggct cgacgcgacc
     1681 atgacggtga gcaagggcac atatatccgc gccctcgccg tcgagctcgg ccgccgcgcc
     1741 gggatcccgg cgcacctcgg ggccctgcgt cgcctggcca gcggcagcct cgcgctcgac
     1801 gacccgcgga ccgtccgcga catcgtctgt gagccgctgc cggacccgct gccgggcctg
     1861 ccgccgaagc ttcgcctgcg ctggccggcc gccgacagcc cacgcgacgc cacccgtgag
     1921 gcgctgcggg ccgccctgat cgacccctgc gacgccctcg acctcccgcg cgtggcgatc
     1981 gccgcggacg atccccgcat gtccgcgctc acgcgcctgc gttgcgggca gagcgtcgcc
     2041 gtcgacgccg cgttcgccga cctgcctgcc gggcggatcc tcggcggtga gggcctcgcg
     2101 ccggccgccc gcctggtcct gcacgacgct gcgggcctcg tcatcgtccg ccgcgaggtc
     2161 acggaggacg gcgaacgcct gcgcccggag cgggtcctcg ccccggcgac gcccgccggc
     2221 gccgcccggg aatgaccgaa accccgcgca cggcgcttga caactccgcc cgccaccccg
     2281 attctccggg tcgcaaaagg accgtttttt cttccggagg catgggcctc caccgagccg
     2341 caaggcccac ccgggcctgt ttcaactacg agccatcatg tccctttctg tcgatcgcaa
     2401 ggccgaggtt gtcaccaagt tccagaccca cgagggcgac accggttcgc ccgaggtcca
     2461 ggtcgcgctg ctgaccaccc gcatcaacta tctcaccgag cacttccgga cccacgcgaa
     2521 ggacttccat tcgcgccgtg gcctgctcaa gctggtcggc cagcgccgcc gcctcctcga
     2581 ctacctgcga ggcaccagcg tcgaccgcta ccgcagcctc atcggggcgc tcaacatccg
     2641 caagtagtgt tccctggggc gcctgaccgg cgcccctttt ctcttatcca gtccgcaagc
     2701 ggccgcgtct gatgcactcc cgtgcgtcgc ggcccgggcg gcccgtgcac ccaagccatg
     2761 gccgagcagc cgtgggatcg ccgtggtttg cgcgcaggcc gagtcccgca acgaattcct
     2821 cctccgggcc gctcgtgccc gatctcctcc cacggcggaa agctccgcca tcatgaacgt
     2881 catcactgaa agctgctcga tcggcgagat cgagatcagc atcgagaccg gcaagctcgc
     2941 caagcaagcc aactccgtcg tcatccgctg cggcgagacc tcggtgctgg tcaccgccgt
     3001 gtcgcaaaag gacccgaagg acctgccctt cctgccgctc acggtcgagt accgcgagta
     3061 caacgccgcc gcgggcaaga tccccggcgg ttacttcaag cgcgagggtc gattgacgga
     3121 tcaggagacc ctgacctgtc gcttcatcga tcgcccgatc cgcccgctct tccccaagag
     3181 ctaccgcaac gacacgcaga tcatcggcac cgtgctctcg cacgacaagg tgcacgagcc
     3241 cgacgtgctc gctatcaccg gcgcctccgc tgccctgatg atcagcgacg ccccgtgggc
     3301 cggcccgatc gccggcgtcc gcgtcgctcg cgtgcagggc aagttcgtcg ccttcccgtc
     3361 gtgggatcag atcaaggtcg ccgacatcgt cgtcatcgtc gccgtcagcc gtgacgcgat
     3421 cgtgatggtc gagggcggcg ccgaccaggc cagcgagtcg gacatgatcg acgcgctgat
     3481 gttcgccaag gaggccgccg cgccgctgat cgccctgcag gaccgcctgc gcgagcgcgc
     3541 cggcaagccc aagcgccagc acgtcgagcc gaccgtcgac ctcgacttcc gcggtcaggt
     3601 gatcgccgcc gccgaggccg gcctgtccgc cgcgttcgcg atcacgggca aggccgagcg
     3661 ccgcgccgcg atcgacaagg ccgtcaaggc cgtcagcgag aagttcctcg ccgacgcccc
     3721 gacgcgcaag gccgaggtcg agtccgcgat ctcgaagctc gagaagaaga tcgtccgcag
     3781 c
//
LOCUS       DASWNJ010001550         1831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_593089, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001550 DASWNJ010000000
VERSION     DASWNJ010001550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593089"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /locus_tag="VGB85_25735"
     CDS             <1..1278
                     /locus_tag="VGB85_25735"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEY0137522.1"
                     /translation="LRRRHLLHGLGIGLVAAPFLNLLRARRTHAEGPAARRLIVFFSP
                     NGTVPQHWRPEGSENAFSFPPGSILEPLTSVQDKVIVVDGLDFLGADNHEPGMAAMLT
                     GNGGASDESGGMSIDQYVAGKIGQDSRFASLEFGVQTSAWGAGAQTRMSYSGPGAWVS
                     PDDDPGHVYGRLFGDLMGGDDAAAALRLRRQRVVDLLVDEATALRARLGAEERPKLDA
                     HLEALAKVEKGLMGGSGCAAPDAPDPLNVGDNASFPAITKAQIDMMVTALACDMTRVA
                     SLQCSHTVSPTVPTWLDIADGHHSLSHIDDSNVAGVAQFVKAERWFAEQFAYLVQSLA
                     DTPEADMQGSMLDNSVVVWAKEMGDSRLHTCKGVPFVLAGGAAGRWKTGRYLQVAGQT
                     HGKLLVSLCQAMGLETATYGNPAGGTGPLPGLV"
     gene            1292..>1831
                     /locus_tag="VGB85_25740"
     CDS             1292..>1831
                     /locus_tag="VGB85_25740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137523.1"
                     /translation="MRTNPRTLTVPFALLIAACNGGGAAVDTEGSSTGSGSGGESATD
                     STPTGGATDGPDTAGPGDCPSDAEFFETHVWQPILSKTCVACHIETGLAKDTRMVFLP
                     AAAEGAAEHNLVEAREVAVLDVDGTSLLLLKPTNKHPDMHKGGAVVQEGSAEYEALAQ
                     FVERSLGTFTCEDPMGEDAA"
BASE COUNT          277 a          701 c          609 g          244 t
ORIGIN      
        1 ctgcgccgaa gacatctgtt gcacgggctc ggcatcggtc tggtcgccgc gcccttcctc
       61 aacctgctcc gcgcccggcg gacgcacgcc gaggggcccg ccgcgcgccg gctgatcgtg
      121 ttttttagcc ccaacggcac ggtgccgcag cactggcgcc ccgagggcag cgagaacgcc
      181 ttcagctttc cgcccgggtc gatcctcgag ccgctgacca gcgtgcagga caaggtgatc
      241 gtcgtcgacg gcctcgactt cctcggcgcc gacaaccacg agcccggcat ggccgcgatg
      301 ttgaccggca acggcggcgc ctccgacgag tccggcggca tgtcgatcga tcaatacgtc
      361 gccggcaaga tcggccagga cagccgcttc gcctcgctcg agttcggcgt ccagaccagc
      421 gcctggggag ccggcgcgca gacccgcatg tcgtactcgg gccccggcgc gtgggtcagc
      481 cccgacgacg accccggcca cgtctacggc cgcctgttcg gcgacttgat ggggggtgac
      541 gacgccgccg ccgccctgcg gctccgccgg cagcgcgtcg tcgacctgct ggtcgacgag
      601 gcgaccgccc tgcgcgcgcg cctcggcgcc gaggagcggc ccaagctcga cgcccacctc
      661 gaggcgctcg ccaaggtcga gaagggcctg atgggcggca gcggctgcgc ggccccggac
      721 gcccccgacc cgctcaacgt cggcgacaac gccagcttcc cggcgatcac gaaggcgcag
      781 atcgacatga tggtcacggc gctcgcgtgc gacatgaccc gcgtcgcctc gctgcagtgc
      841 tcgcacaccg tcagcccgac cgtgccgacc tggctcgaca tcgccgacgg ccaccactcg
      901 ctgtcccaca tcgacgactc caacgtcgcc ggcgtcgccc agttcgtcaa ggccgagcgg
      961 tggttcgccg agcagttcgc ctacctcgtg cagagcctcg ccgacacccc cgaggccgac
     1021 atgcagggct cgatgctcga caactccgtc gtcgtgtggg ccaaggagat gggcgactcg
     1081 cggctgcaca cctgcaaggg cgtgcccttc gtgctcgccg gcggcgccgc ggggcgctgg
     1141 aagaccgggc gctaccttca ggtcgccggc cagacgcacg gcaagctgct cgtctcgctg
     1201 tgtcaggcga tgggcctcga gacggcaacc tacggcaacc ctgcgggcgg caccggcccg
     1261 ctccccggac tggtctgaga cggaggacac catgcgcacg aatccccgca cgctcactgt
     1321 cccgtttgct cttctgatcg ccgcctgcaa cggcggcggc gcggccgtcg acaccgaagg
     1381 cagcagcacc ggctccggct ccggtggtga atctgccacc gacagcaccc cgaccggggg
     1441 tgccaccgac ggtcccgaca ccgccggtcc gggcgactgc ccgagcgacg ccgagttctt
     1501 cgagacccac gtctggcagc cgatcctcag caagacctgc gtcgcctgtc acatcgagac
     1561 cggcctcgcc aaggacacgc gcatggtgtt cctgccggcc gcggccgagg gcgccgccga
     1621 gcacaacctc gtcgaggccc gcgaggtcgc ggtcctcgac gtcgacggca cctcgctgct
     1681 gctgctcaag ccgaccaaca agcaccccga catgcacaag ggcggcgcgg tggtccagga
     1741 gggcagcgcc gagtacgagg cgctcgcgca gttcgtcgag cgcagcctcg gcaccttcac
     1801 ctgcgaggat ccgatgggcg aggacgcggc c
//
LOCUS       DASWNJ010001551         3585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_559443, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001551 DASWNJ010000000
VERSION     DASWNJ010001551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559443"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /locus_tag="VGB85_25745"
     CDS             complement(<1..1419)
                     /locus_tag="VGB85_25745"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY0137524.1"
                     /translation="MIVAVVLPLLWLLPLLAAAVVQGLLHDQRQQRALAIATSWVVFG
                     LAVLLLIVNGSGDAFARDDVEAGPVFLSLHYHMAVDGLNAVLLPMTAAFSLAALLSAP
                     RSDLRGDTTARILVVEAALLGSFLSMDAALMSLFWIASMLPLWQDTRRSGSRPLHVIM
                     TMSLAATVVSLLGAVALLASHGISLGLRAPLDLIAISRADVAPPELVGWLFLFAGLLR
                     MGILPLHLWLTAAAARAPGYSAMLALFTPLGSFALARVVIPLFPHVCEQFSAPLVTLG
                     AGTALYGALLAVGQHDLRRVLGYFWVSQMGLVLAGLAALNAPSVAGALMQSMSSVVEV
                     GGLMLIALEVEARAGTTDMRLLGGLVRHAPRMATGFFLLSAAAVGFPGTISFVAEDLA
                     IQGLLRQHPIVAGVLLVVTAINGIVLFKAGKRVFLGPPSPHAVALRSFEDLRGWEYLA
                     SVAMMGTLLVGGFMPEPLLAVRS"
     gene            complement(1425..2759)
                     /locus_tag="VGB85_25750"
     CDS             complement(1425..2759)
                     /locus_tag="VGB85_25750"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY0137525.1"
                     /translation="MPDLDLLCMFVALTPVWPALGAVLIALLMLPRGRPSERLVARVG
                     RVSLILSLLGALAGVALRFLGGPVDYRLGDWYHAADVSFPLVFLFDGLSASVSLLIAI
                     LLLTTSNFSRQYLHREPGFIRFFLLMLCFGAGMHLLVLAGSVELLFIGWELVGITSVL
                     LVAFFHERAGPVQAAIRVLVTYRLCDIGLLLSGVFLHQWLHTTVWVDTFRGAETAGGG
                     LAATAIGLGLVFAAMGKSAQFPVGGWLPRAMEGPTASSAVFYGSLSVHAGVYLLLRAA
                     PLIEHSPAARIALVIVGAVTAVMAALSSQVSADAKSAVAYATASQVGLMFVACGLGFY
                     TLATVHLIAHATLRYYQFLRTPSALQDALARRAALGRTEQDEAAALWEGVGLKQRRFL
                     YRLALERFAVETALDRWITRPIMGLSRWLDGLENRFLALHDRPVPARKGDLP"
     gene            complement(2752..>3585)
                     /locus_tag="VGB85_25755"
     CDS             complement(2752..>3585)
                     /locus_tag="VGB85_25755"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative inorganic carbon transporter subunit
                     DabA"
                     /protein_id="HEY0137526.1"
                     /translation="GDRVRFFDVETLAPAARSELTRLEQLLAEMCRRNAHERCRRFES
                     APPRPTPEQARQHVEERALDLSQARPELGHATNAACIVGRRALSQGLFLDRRAFLVSY
                     DPSIDPTGAVLERTLAAVGPVGAGINLEYYFSTVDGERLGSGTKLPHNVTGLFGVMNG
                     AASDLRTGLPKQMTEIHEPVRLHLVVEATPEMLTQVCERQPGVAELVMGEWVRLAAVH
                     PETRQIFTFDARHGFTPYTPQNPTLPSVSRSRDWYAGHDGFLAPIRVAPRELMVEPFH
                     A"
BASE COUNT          628 a         1141 c         1436 g          380 t
ORIGIN      
        1 gctgcggacc gcgagcagtg gttcgggcat gaagccgccg accagcaggg tgcccatcat
       61 ggcgaccgag gcgaggtact cccagccgcg gaggtcttca aagctgcgca gggcgacggc
      121 gtggggggag ggtgggccga ggaacacgcg cttgccggcc ttgaacagga cgataccgtt
      181 gatcgcggtg acgacgagca gcacgccggc gacgatcggg tgctggcgca gcaggccctg
      241 gatcgccaga tcctcggcga cgaaggagat cgttccgggg aagccgacgg cggcggccga
      301 gagcaggaag aagccggtcg ccatgcgcgg ggcgtgacgc acgaggccgc cgagcaggcg
      361 catgtcggtg gtgccggcgc gggcctcgac ctcgagcgcg atcaacatca gaccgccgac
      421 ctcgaccacc gaggacatcg actgcatgag cgcaccggcg acgctggggg cgttgagagc
      481 ggcgaggccg gcgagcacca ggcccatctg cgagacccag aagtagccga ggacccggcg
      541 caggtcgtgc tggcccacag ccaatagggc gccgtacaag gccgtgccgg cgccgagggt
      601 cacgagcggc gcggagaact gctcgcacac gtgggggaac agcgggatga cgacgcgggc
      661 gagcgcgaac gagccgagcg gcgtaaacag ggcgagcatc gccgagtagc cgggggcccg
      721 tgcggcggct gcggtgagcc acaggtgcag cggcaagatg cccatgcgca gcaggccggc
      781 gaacaggaac agccagccga cgagctcggg cggggcgacg tcagcgcgcg agatggcgat
      841 caggtcgagc ggggcgcgca ggccgagcga gatgccgtgg ctggcgagca gggcgacggc
      901 gccgagcagc gagacgacgg tggcggcgag cgacatcgtc atgatcacgt gcagcgggcg
      961 tgaaccactg cggcgcgtgt cctgccacag cggcagcatc gacgcgatcc agaacagcga
     1021 catcagggcg gcgtccatcg acaggaagct gccgagcagc gcggcctcga cgacgaggat
     1081 ccgcgcggtc gtgtcgccgc gcaggtcgga gcgcggcgcc gacagcagcg cggcgagcga
     1141 gaaggcggcg gtcatcggca gcagcacggc gttgaggccg tcgacggcca tgtggtagtg
     1201 cagcgacagg aacaccgggc cggcctcgac gtcgtcgcgc gcgaaggcgt cgcccgagcc
     1261 gttgacgatc aacagcagca ccgcgaggcc gaacaccacc caagaagtgg cgatcgcgag
     1321 cgcgcgctgc tggcgttggt cgtgcagcag gccctggacg acggcggccg ccaacaacgg
     1381 cagcagccac agcagcggga gcacgacggc gacgatcatg tgcgtcacgg caggtccccc
     1441 ttgcgcgccg ggaccgggcg gtcgtgcagc gcgaggaagc ggttctcgag gccgtcgagc
     1501 cagcgcgaca ggcccatgat cgggcgcgtg atccagcggt cgagggcggt ctcgacggcg
     1561 aagcgctcga gcgcgaggcg gtagaggaag cgccgctgct tgaggccgac gccctcccac
     1621 agcgcggcgg cctcgtcctg ctcggtgcga ccgagcgcgg cccggcgcgc gagcgcgtcc
     1681 tgcagcgccg agggggtgcg caggaattga tagtagcgca gcgtcgcgtg ggcgatcagg
     1741 tgcacggtgg cgagggtgta gaagccgagg ccgcaggcga cgaacatcag gccgacctgg
     1801 ctggcggtcg cgtaggcgac ggcggacttg gcgtcggcgc tgacctggct gctgagcgcg
     1861 gccatcacgg ccgtgacggc gccgacgatc acgagcgcga tcctggcggc gggcgagtgc
     1921 tcgatcagcg gcgcagcacg taacagcagg tagacgccgg cgtgcacgga gaggctgccg
     1981 tagaacacgg cgctcgaggc ggtcgggccc tccatcgcgc gcgggagcca gccgccgacc
     2041 gggaactggg ccgacttgcc catggcggcg aacacgaggc cgaggccgat cgcggtcgcc
     2101 gcgaggccgc caccggccgt ttcggcgccg cggaaagtgt cgacccacac ggtggtgtgc
     2161 agccactggt gcaggaacac gcccgacagc agcaggccga tgtcacacag gcggtaggtg
     2221 acgagcacgc ggatcgcggc ctggaccggg ccggcgcgct cgtggaagaa ggcgacgagc
     2281 agcaccgagg tgatgccgac gagctcccag ccgatgaaca gcagctcgac gctgccggcg
     2341 aggaccagca ggtgcatgcc ggcgccgaag cacagcatca gcaggaagaa gcggataaag
     2401 ccgggctcgc ggtgcaggta ctggcgcgag aagttggagg tcgtcagcag caggatcgcg
     2461 atcagcagcg acacggaggc cgagaggccg tcgaacagga acaccagcgg gaagctgacg
     2521 tcggcggcgt ggtaccagtc gccgaggcgg taatccaccg gcccgccgag gaagcgcagc
     2581 gcgaccccgg cgagggcgcc gagcagcgag aggatcagcg agacgcgacc gacgcgggcg
     2641 acgaggcgct cggaggggcg gccgcggggc agcatcagca gggcgatcag cacggcgccg
     2701 agggcgggcc acaccggcgt cagcgcgacg aacatgcaca gtaagtcgag gtcaggcatg
     2761 aaagggctcg accatgagct cgcgcggggc gacgcggatg ggggcgagga agccgtcgtg
     2821 gccggcgtac cagtcgcgcg agcggctgac gctcggcagg gtcgggttct gcggggtgta
     2881 gggcgtgaag ccgtggcgcg cgtcgaaggt gaagatctgg cgggtctcgg ggtgcacggc
     2941 ggcgaggcgc acccactcgc ccatcaccag ctcggcgacc ccgggctggc gctcacacac
     3001 ctgcgtcagc atctccgggg tggcctcgac gaccaggtgc aggcgcactg gttcgtggat
     3061 ctccgtcatc tgcttcggta ggccggtgcg caggtcgctg gcggccccgt tcatcacgcc
     3121 gaacaggccg gtgacgttgt gcggcagctt ggtgccggag ccgaggcgct cgccgtcgac
     3181 ggtcgagaag taatactcga ggttgatgcc ggcgccgacc gggccgaccg ccgcgagggt
     3241 ccgctcgagc accgcgccgg tcgggtcgat gctcgggtcg taggagacga ggaacgcccg
     3301 ccggtcgagg aacaggccct gcgacagcgc gcgccggccg acgatgcagg ccgcgttggt
     3361 cgcgtggccg agctcgggcc gcgcctggct gaggtcgagc gcgcgctcct cgacgtgctg
     3421 gcgcgcctgc tccggggtcg gacgcggcgg cgcggactcg aagcgacggc agcgctcgtg
     3481 ggcgttgcgg cggcacatct cggcgagcag ctgctcgagc cgcgtcagct cggagcgggc
     3541 cgcgggggcg agggtctcga cgtcgaagaa gcggacccgg tcgcc
//
LOCUS       DASWNJ010001552         1987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_559479, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001552 DASWNJ010000000
VERSION     DASWNJ010001552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559479"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VGB85_25760"
     CDS             <1..271
                     /locus_tag="VGB85_25760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137527.1"
                     /translation="MSLHSYLVDTDKYLMHSHIPSSEGGDIPFDLEYVDVASAKYTLI
                     AGAVSAAIQVPIQGFGTEVEPGVESHVLYLDAHGAEPGVWVAPLP"
     gene            complement(281..>1987)
                     /locus_tag="VGB85_25765"
     CDS             complement(281..>1987)
                     /locus_tag="VGB85_25765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137528.1"
                     /translation="VDLDAGAFATIPGPGTPAIVAASKQHTPSPDGRIVAAVHERERL
                     VIWDREDGSVRSVRLPAKFTFHSIEFAEDARTLVMHSSGERVLVWRDDGTTRVIDAGW
                     ATFAGETLLLLGSAERGLKLWAEPITGGPPLWSRSLSDEFPASRAWRPFMAGAHGLAR
                     VQLAGRWEVFDFRTGAVRYARAGNYDEPLLLGHGEQILLTAPGEDAVHRLQLLDLSRS
                     PWTPLAPLAASGGIVRTALSASARFAVRQHLKSRVVERLDLTRGVAEPLPAGCGAWTS
                     DPLLLAAGDDGRVLLADNQFHACLWSGDVTYPLPVSQGFVSGMALAPDGAGFAIAIAD
                     NTLFSWDRPDAEPRRGAGTQMKYSPDGEYMVVLADPDVTILPRSGAPIALPKLGGAHG
                     ITIAISPDSRQIALHTRTAGAVHLVDARDGKTVRTFDAPPPIDLSTGGQGPRFSPSGR
                     VLALADGDRMYRWDIDGSPEPVYLDRVGAVGFAFTADEQALLAIDNYGNLLLVDPIAG
                     VQTTLRSESSRGAPPWMVAMPDGAVLMTTAGEVLRWQDMLPARGDALRGWLRASAAAL
                     AP"
BASE COUNT          312 a          683 c          724 g          268 t
ORIGIN      
        1 catgagcctc cattcgtacc tggtagatac ggacaagtat ctgatgcact cccacatccc
       61 gtcgtccgag ggaggcgaca tcccgttcga cctcgaatac gtcgatgtcg ccagcgcgaa
      121 atacaccctg atcgccggcg cggtctcggc cgccatccag gtccccatcc agggattcgg
      181 gaccgaggtc gagccgggcg tggagagcca tgtgctctac ctcgacgccc acggcgccga
      241 gccgggcgtg tgggtcgcgc cgctgccgtg accgcggagc ctacggagcg agggcagccg
      301 cgctggctcg cagccagccg cgcagcgcgt cgccgcgggc cgggagcatg tcctgccagc
      361 gcagcacctc gccggcggtc gtcatcagca cggcgccatc gggcatggcg accatccacg
      421 gcggggcgcc gcgactggac tcgctgcgca gggtcgtctg cacgccggcg atgggatcga
      481 cgagcaggag atttccataa ttgtcgatcg ccagcagggc ctgctcgtcg gcggtgaagg
      541 cgaagccgac ggcaccgacg cgatcgaggt aaacgggctc cggcgatccg tcgatgtccc
      601 agcgatacat cctgtccccg tcggcgagcg cgagcacgcg acccgagggg gagaagcgcg
      661 gaccctggcc gccggtcgag aggtcgatcg gcggtggagc gtcgaaagta cgcacggtct
      721 tgccgtcgcg cgcgtcgacg aggtgcaccg cgccggccgt gcgggtgtgt agcgcgatct
      781 gccggctgtc cggtgagatg gcgatcgtga tgccatgcgc ccccccgagc ttcggcagcg
      841 cgatcggtgc accgctgcgc ggtaggatcg tcacatcggg gtcggcgagg accaccatgt
      901 attcgccgtc ggggctgtat ttcatctgcg tgcccgcgcc gcgccgcggc tcggcgtccg
      961 ggcggtccca ggagaacagc gtgttgtcgg cgatcgcgat cgcaaagccg gcgccgtcgg
     1021 gcgcgagtgc catgccggac acgaagccct gcgagaccgg gagcgggtag gtcacatcgc
     1081 cgctccacag acaggcgtga aactgattat cggcgagcag gacccggccg tcgtcgccgg
     1141 cggccagcag gagcgggtcg gaggtccacg cgccgcagcc cgcgggcagc ggctcggcga
     1201 cgccgcgggt cagatcgagg cgctcgacga cgcggctctt gaggtgctgc cgaaccgcga
     1261 agcgagcgct cgcggagagt gcagtgcgaa cgatgccgcc ggacgcggcg agcggggcga
     1321 gcggggtcca cggcgagcgc gacaggtcga gcagttgcag gcgatgcacg gcatcctcgc
     1381 cgggcgcggt cagcaagatc tgctcgccgt ggcccagcaa cagcggctcg tcgtaattgc
     1441 cggcgcgggc gtatcgcacg gcgcctgtac gaaagtcgaa gacctcccag cggccggcga
     1501 gctgcacgcg ggcgaggcca tgtgcgccgg ccatgaacgg ccgccacgcc cgcgacgccg
     1561 gaaattcatc gctcaacgat cggctccata gcggcgggcc gccggtgatc ggctcggccc
     1621 acagcttcag gcccctctcg gcggacccga gcagcagcag cgtctcgccc gcgaacgtgg
     1681 cccagcccgc gtcgatgacg cgggtggtgc cgtcgtcacg ccataccagc acgcgctcgc
     1741 ccgacgagtg catgacgagc gtgcgcgcat cctcggcgaa ctcgatgctg tggaacgtga
     1801 atttcgcggg cagacggacg ctgcgaaccg agccatcctc ccggtcccag atcaccaggc
     1861 gctcgcgctc gtgcaccgcg gcgacgatgc gaccgtcggg cgacggcgtg tgctgcttgc
     1921 tcgccgcgac gatcgccggc gtcccggggc ccgggatcgt ggcgaaggca ccggcgtcga
     1981 ggtcgac
//
LOCUS       DASWNJ010001553         3750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_205274, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001553 DASWNJ010000000
VERSION     DASWNJ010001553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205274"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1583)
                     /locus_tag="VGB85_25770"
     CDS             complement(<1..1583)
                     /locus_tag="VGB85_25770"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0137529.1"
                     /translation="MSLTELIAALARPEALGHPPDATVEVVHTHVSVVLLVGDDVYKF
                     KKPQDLDFLDYSTLDRRRHFCELEVTLNRRLAPDVYLGVVEIRRDDAGELRVEGPGEV
                     VEVAVHMRRLPDAAALACRVEQPADDPRGVDAAALRTIGARVLGFHRAARRGPEVSAW
                     ATFAVVAANARQNFAQLADLGRVSEALLTRLERCTEEALGRHHATIEARARRRIACDT
                     HGDLRLEHIYLFPERPPPGDLVILDCIEFAEQFRCADPIADLAFLVMDLHRVGRRDLA
                     TALLAGYFAAYDAPLAALPDSTGDVADERAEARALLPLYTAYRATVRAKVEAFKAAEP
                     EVPPAVAAAAAVAARRYLLLALYELQAPALRPCLVLVAGLPGTGKSTLARALAAEGFG
                     WVRSDAVRKQLAGLPPEAPAHAATGAGIYTAAWTDRTYARCHDLARAALSEGERVVVD
                     ANFKTAAQRAPFVALARELGVPLQDFVCEAEPAAVEERLSRRTGDVSDAGITVYRHAR
                     AAWEPVALDLDATVVDTTGP"
     gene            complement(1731..3356)
                     /locus_tag="VGB85_25775"
     CDS             complement(1731..3356)
                     /locus_tag="VGB85_25775"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEY0137530.1"
                     /translation="MRLERNLLLSLALTAVACGDDGPALLTEASTGDTTGTTTDGSST
                     IDPTTTPTTDTPTGTDANTEPTTQGPTTDTPETTTGNTTGDTTVGDTSTGTTSGDTTG
                     DTDGTTGTTTGDTTGGTTSGDTTGGTTGGDTEGTTGDTGDTDTMGAVEDEIYEIQNGT
                     IAEATMVTVEGVIVTGIHPGKTGLFVQEPGGGEYSGVWVYVGNMGPDIATLAVGDEVD
                     VSGVTVEFNELTEIDASAGTVTATGVQNLTVDAELLPIDTFSDPVTAEPWEGVFVGIL
                     GQPLDLTAVMGTEYTLTEAGSSLVVDDLLYASLMDQIAFPQIGVGATFDAAFGPLNQS
                     GPVYKIAPRTSADLEGYVPPMNPTLGIQAVKAGDLVISEVMYNPTCANDDCEWIEIYN
                     ASASAIDLNGLIIQNNQQNKNLQGVINKSVVVQPGSFTILGAETMVTWPYPNPPAAFY
                     GANPPLGNGVNGDQVFLKNNTITIDGMPKWLSQAGKDSGYSFKVAPDKLNSIDNDLLA
                     NWCYSTVVFYTNPMMVTEKGSPGAANEAACAVL"
     gene            3484..>3750
                     /locus_tag="VGB85_25780"
     CDS             3484..>3750
                     /locus_tag="VGB85_25780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137531.1"
                     /translation="MSQLPHKFFIDSHKASNAVAVVAMMAYFDAWHNETAWTYLALHG
                     TYGFLWLTKSQVFPDKQWERPVSWSLGIQTWAFLTLYWVSPYLIT"
BASE COUNT          500 a         1191 c         1470 g          589 t
ORIGIN      
        1 gggccggtgg tgtcgacgac ggtggcgtcg aggtcgaggg cgaccggctc ccacgcggcg
       61 cgggcgtggc gatacacggt gataccggcg tccgagacgt cacctgtcct tcgggacagg
      121 cgctcctcga ccgccgccgg ctcggcctcg cagacgaagt cctgcagcgg cacgccgagc
      181 tcgcgcgcga gcgcgacgaa gggggcgcgc tgggccgcgg tcttgaagtt ggcgtcgacg
      241 acgacgcgct cgccctccga cagcgcggcg cgggcgaggt cgtgacagcg cgcgtaggtg
      301 cggtcggtcc aggccgcggt gtagatgccg gcgccggtgg ccgcgtgcgc gggggcctcg
      361 ggtgggagcc cggcgagctg cttgcgcacc gcgtcggagc ggacccagcc gaagccctcg
      421 gccgcgagcg cgcgcgcgag cgtcgacttg cctgtccccg gcaggccggc gacgagcacg
      481 aggcacgggc gcagcgcggg cgcctgcagc tcgtacagcg cgagcagcag gtagcggcgg
      541 gcggcgaccg cagcggccgc ggcgacggcg ggcgggacct cgggctcggc ggccttgaag
      601 gcctcgacct tggcgcgcac ggtggcccgg taggcggtgt aaagcggcag cagagcgcgg
      661 gcctcggccc gctcgtcggc gacgtcgccg gtggagtcgg gcagcgcggc gagcggggcg
      721 tcgtaggccg cgaagtagcc ggcgagcagg gcggtggcga ggtcgcggcg accgacgcgg
      781 tgcaggtcca tcaccaggaa ggcgaggtcg gcgatcgggt cggcgcagcg gaactgctcg
      841 gcgaactcga tgcagtcgag gatcaccagg tcgccgggcg gcgggcgctc ggggaacagg
      901 tagatgtgct cgaggcgcag gtcgccgtgg gtgtcgcagg cgatccgtcg ccgcgcccgc
      961 gcctcgatcg tggcgtggtg ccggccgagc gcctcctcgg tgcagcgctc gaggcgggtc
     1021 agcagcgcct cgctgacgcg cccgaggtcg gccagctgcg cgaagttctg gcgcgcgttg
     1081 gcggcgacga cggcgaaggt ggcccacgcg gagacctcgg gcccgcgacg ggcggcgcga
     1141 tgaaagccga gcacgcgcgc gccgatcgtg cgcagcgcgg cggcgtcgac cccacgtgga
     1201 tcgtcggccg gctgctcgac gcggcaggcc agcgcggcgg cgtcgggcag gcggcgcatg
     1261 tgcaccgcga cctcgacgac ctcgccggga ccctcgacgc ggagctcacc ggcgtcgtcg
     1321 cgccggatct cgaccacgcc gaggtagacg tccggggcga ggcgacggtt gagcgtgacc
     1381 tcgagctcgc agaagtgccg gcggcgatcg agcgtcgagt agtcgaggaa gtcgaggtcc
     1441 tgcggcttct tgaatttgta cacgtcgtcg ccgacgagca gcacgaccga gacgtgcgtg
     1501 tgcacgacct cgacggtcgc gtccggcgga tgaccgagcg cctcgggccg cgcgagcgcg
     1561 gcgatcagct cggtcagcga catcggcgcg aggctgccac ggcgaggcgg cggcggcaag
     1621 cgttcgtccg cgggcacgcg cacgggcgaa tgcccggtgc gcccgcaaaa gcgcgcgacc
     1681 gggccgatcg agagggggcc acgcggcccc ggtctcacgc gctcagcggc tcacagcacc
     1741 gcgcaggcgg cctcgttggc ggcgccgggc gagccctttt cggtgaccat catcgggttg
     1801 gtgtagaaca cgacggtcga gtagcaccag ttggcgagca ggtcgttgtc gatcgagttg
     1861 agcttgtcgg gggcgacctt gaacgagtag cccgagtcct tgcccgcctg gctgagccac
     1921 ttgggcatgc cgtcgatcgt gatcgtgttg ttcttgagga acacctgatc accgttgacg
     1981 ccgttgccga gcggcgggtt ggcgccgtag aacgcggccg gcgggttggg gtacggccag
     2041 gtgaccatcg tctcggcgcc gaggatggtg aagctgcccg gctgaacgac gacggacttg
     2101 ttgatcacgc cctggaggtt cttgttctgc tggttgttct ggatgatcag gccgttgagg
     2161 tcgatcgccg aggcggaggc gttgtagatc tcgatccact cgcagtcgtc gttggcgcag
     2221 gtcgggttgt acatgacctc gctgatcacg aggtcgccgg ccttgacggc ctggatgccg
     2281 agcgtcgggt tcatcggcgg cacatagccc tcgaggtcgg cgctggtgcg cggggcgatc
     2341 ttgtagaccg ggccggactg gttcaggggg ccgaacgcgg cgtcgaaggt cgcgccgacg
     2401 ccgatctgcg ggaacgcgat ctggtccatc agcgaggcgt agagcaggtc gtcgacgacg
     2461 agcgagctgc ccgcctcggt cagcgtgtac tcggtgccca tcacggcggt caggtcgagc
     2521 ggctgcccga ggatcccgac gaacacgccc tcccacggct cggcggtgac cgggtcgctg
     2581 aaggtgtcga tcggcagcag ctcggcgtcg acggtcaggt tctgcacgcc ggtcgcggtg
     2641 acggtgccgg cgctggcgtc gatctcggtc agctcgttga actcgaccgt cacgccgctg
     2701 acatcgacct cgtcgccgac ggcgagcgtg gcgatgtccg ggcccatgtt gccgacgtag
     2761 acccagacgc cgctgtactc gccgccgccc ggctcctgca cgaacaggcc ggtcttgccg
     2821 gggtggatgc cggtgacgat gacgccctcg acggtgacca tggtcgcctc ggcgatcgtg
     2881 ccgttctgga tctcgtagat ctcgtcctcg acggcgccca tcgtgtcggt gtcgccggtg
     2941 tcgccggtcg tgccctcggt gtcgccgccg gtggtgccgc cggtggtgtc acccgaggtg
     3001 gtgccgccgg tggtgtcgcc agtcgtcgtg ccggtggtgc cgtcggtgtc gccagtggtg
     3061 tcgcccgagg tcgtgccggt cgaggtgtcg ccgacggtgg tgtcgccggt ggtgttcccg
     3121 gtggtcgtct cgggggtgtc cgtggtcgga ccctgcgtcg tcggctcggt gttcgcgtcg
     3181 gtgccggtcg gcgtgtcggt ggtcggcgtg gtcgtcgggt cgatggtcga cgagccgtcc
     3241 gtggtcgtgc cggtggtatc gccggtggag gcttcggtga ggagcgcggg gccatcgtcg
     3301 ccacaggcga ccgcggtgag cgcgagagag aggagcaggt tacgttcaag gcgcatgtcg
     3361 cggacgctag cccccggcgg caggtagctc aagtgacttt ggggcatcat tcgcgcgtta
     3421 acacaccttg gctgtgagca acgtcggtgt ggcaccatac ggtcgatccg gaggtcctca
     3481 cgcgtgtcac agctcccgca caaattcttc atcgacagtc acaaggcgag caacgcggtc
     3541 gcggtcgtcg cgatgatggc gtacttcgac gcctggcata acgagacggc gtggacgtac
     3601 ctcgcgctgc acggcaccta cgggttcctg tggctgacca agagtcaggt gttcccggac
     3661 aagcagtggg agcggcccgt gtcgtggtcg ctggggatcc agacctgggc gttcctgacg
     3721 ctgtactggg tgtcgccgta cctgatcacg
//
LOCUS       DASWNJ010001554         5268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_63873, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001554 DASWNJ010000000
VERSION     DASWNJ010001554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63873"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VGB85_25785"
     CDS             <1..593
                     /locus_tag="VGB85_25785"
                     /inference="COORDINATES: protein motif:HMM:NF024747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S single cluster domain-containing protein"
                     /protein_id="HEY0137532.1"
                     /translation="PFRRAALWVQGCSLACPGCCNPELFLKDGGTQRSIAALVDELRK
                     HREQHAIEGLTVLGGEPSEQLPSVTALCREARRLGLGVIVFTGRTEAELAALPGGPAL
                     LATVDTLVDGRFDATCREPADGRRFIGSSNQRLVHRSPRYADPALWRGPRGVELQLTP
                     DGQISLHGTPALARRLARALAERPEPRAGGPARIFD"
     gene            674..2431
                     /locus_tag="VGB85_25790"
     CDS             674..2431
                     /locus_tag="VGB85_25790"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEY0137533.1"
                     /translation="MVLEARHDNHFSRPEDVEEFISVLERFERGELGSEEFRSFRLTR
                     GVYGQRQMDVNMLRTKFPNGIMSAGQLRACAKIARELTRFDKGHVTTRQNIQFHFVKL
                     EDMPKAMASLDEFGSTTMEACGNTVRNITGCPYAGVSPTEAFDVTPYTHAVTRLCLRA
                     PWANDLPRKFKMAFEGCTHEDHAQTAINDIGLRAKIQDGVPGFEMFVAGGLSTSPRPG
                     YRLHAFIPADELLARIEATIKLFGRLGNRKNRARARLKYVMKNLGEEGFVAAYNEEVD
                     KIVDEGRLKQPIELPPDETAPKADPRALQGPRAPEDDPTYRAWLRTVDPQKQSGFASV
                     TVKLYRGDLSSTQFEQLAEVTEVFADGTLRTMNNQNILLRWVRTEALPHLYSRLQEIG
                     LAEVGSNTIVDVVSCPGAESCNLAVTASMQLAAHLTRFLKNPGKSADSVELARDADIK
                     ISGCPNSCGQHHVAAIGFHGTVRHAGDGRQIPEYQLHLGGGFNAEGVEFGRHIVKIPA
                     QRAGEALLALLDLYRDNREAGEDARSFYRRVPKEKVAAALGEHTIGMSFDNLRETDLV
                     DLGQEKLFDVAIGRGECAG"
     gene            complement(2566..4068)
                     /locus_tag="VGB85_25795"
     CDS             complement(2566..4068)
                     /locus_tag="VGB85_25795"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0137534.1"
                     /translation="MSLSTRVLALVVLTLAVPACKKSGTNAPGGKGVTVEEQDKKRAA
                     AQQDAKARTLVGLANEDLSKGRWFSARERAKRALEAEPNNADAFAILGAANWRAGDYA
                     ASTKAFKEALELDAKNFGAAVGLGRNYQAAGDHKSAIELQDKLLEADPAQFDPLVTKL
                     WSYYALADAANAVKTLDALFKQVDNDNPLRPLLLAHAAFMRPLEGKGELIKVEGARGT
                     SDLQVDPSQGIKHAGGTVGGEFTRIVFFELREEARIHKALADQLKLKELGKITPIAGT
                     ETSVVLIPEIKFGDLKLTNVPAIVEDLTPYTVGEVPGVVLGRQVMNKLGAITFDFPNA
                     SLELAAAAPAATPAGSSEAPLLLIDMHILLVPVTTVSIDGSDFKFYAWLGGSYGAALA
                     VARKEYLKSGHLPRELEQLDDPTNGLKMVFLEKVKVGETAVSGGVGGLVLANTPPDAG
                     LAQIVEGSSFELGGYINYPLLRTMKVTYAMSQGKVYLSGKTAAPTTTASK"
     gene            complement(4147..5220)
                     /locus_tag="VGB85_25800"
     CDS             complement(4147..5220)
                     /locus_tag="VGB85_25800"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEY0137535.1"
                     /translation="MTPGPSEPAPRARLRRDLWLWLLSLVGAGGLLWLASFRIDLWPD
                     EISLPRPGLLVAAAALHLPYSLARALRLRYALDHRVQEATGDRRARVPRDLLYGSGLV
                     SFLVIILLPLRLGELSRPLLLARGGVPGVGLPEAIAAIASERAVDGLIVVGLVLVGVA
                     EATALLPGLGESAASVTQVGRGMGYVFLALVLGLVALARTPARWLARLGAAFPDRLAG
                     FVTQFAATLRDLADLRRGLPLLLWSAVYWALTVVQLYLVLHACGLGLGLAEAAAIVGI
                     VGLSIQLPGGPAQVGSYQVGSVLALSLFVPAADLDAGGASFVAVSFVLGLLGAAVLAV
                     PGAWLLARAGRRARASGSESSEA"
BASE COUNT          849 a         1836 c         1860 g          723 t
ORIGIN      
        1 gcccgtttcg ccgcgcggcg ctgtgggtcc agggctgctc gctcgcctgc cccggctgct
       61 gcaaccccga gctgttttta aaagacggcg gcacacagcg gtcgatcgcc gcgctggtcg
      121 acgagttgag gaaacatcgc gagcagcacg cgatcgaagg cctgacggtc ctcggcgggg
      181 agccgagcga gcaactgccc agtgtcaccg ccctgtgccg cgaggcccgc cggctcggcc
      241 tcggcgtgat cgtgttcacg ggccgcacgg aggccgagct cgccgcgctg cccggtgggc
      301 cggcgctgct ggcgaccgtc gacacgctcg tcgacggccg cttcgacgcg acctgccgcg
      361 agcccgccga cggccggcgc ttcatcggct cgagcaatca acgcctcgtg caccgcagcc
      421 cacgttacgc cgaccctgcg ctgtggcgcg ggcctcgcgg cgtcgagctg cagctgaccc
      481 cggacggcca gatcagtctg catggcacac ccgcgctggc gcggcgcctg gcccgcgcgc
      541 tcgcggagcg accggagccc cgcgctggcg gtcccgcgcg gattttcgat tgaaacgggc
      601 cggcctgtgc tttagtctga acgccgattc actctactga acgacgccac ggcgtcggcg
      661 gagcctgcga gccatggtcc tggaagcgcg tcacgacaat catttttctc gccccgagga
      721 cgtcgaggag ttcatttcgg tgctcgaacg cttcgagcgc ggcgagctcg gctccgagga
      781 gtttcgcagc tttcgtctga cgcgcggcgt gtacggccag cggcagatgg acgtcaacat
      841 gctgcgcacc aagttcccca acgggatcat gagcgcgggg caactgcggg cctgcgccaa
      901 gatcgcgcgc gagctgacgc gttttgacaa gggccacgtc accacgcggc aaaacatcca
      961 gttccacttc gtgaagctcg aggacatgcc gaaggcgatg gcgagcctcg acgagttcgg
     1021 ctcgacgacg atggaggcct gcggcaacac cgtccgcaac atcaccggct gcccgtacgc
     1081 gggcgtcagc ccgaccgagg ccttcgacgt cacgccgtac acccacgcgg tgacgcgcct
     1141 gtgcctgcgc gcgccgtggg ccaacgacct cccgcgcaag ttcaagatgg ccttcgaggg
     1201 ctgcacgcac gaggaccacg cgcagacggc gatcaacgac attggtcttc gcgccaagat
     1261 ccaggacggc gtgccgggct tcgagatgtt cgtcgccggc ggcctgagca ccagcccgcg
     1321 gccgggctac cgcctgcacg cgttcattcc tgcggacgag ctgctcgcca gaatagaggc
     1381 gacgatcaag ctgttcgggc gcctcggcaa ccgcaagaac cgcgcgcgcg cgcgcctcaa
     1441 gtacgtgatg aagaacctcg gcgaggaggg cttcgtcgcc gcgtacaacg aggaggtcga
     1501 caagatcgtc gacgaggggc gcctcaagca gccgatcgag ctcccgcccg acgagacggc
     1561 cccgaaggcc gacccgcggg cgctacaggg cccccgtgct ccggaggacg atcccaccta
     1621 ccgcgcctgg ctgcgcaccg tcgacccgca gaagcagtcg ggcttcgcca gcgtgaccgt
     1681 caagctgtac cgcggcgacc tcagctcgac gcagttcgag cagctcgccg aggtgaccga
     1741 ggtgttcgcc gacggcacgc tgcgcacgat gaacaaccag aacatcctgc tgcgctgggt
     1801 ccgtaccgag gcgctgccgc acctgtacag ccgcctgcag gagatcggcc tcgcggaggt
     1861 cgggtcgaac acgatcgtcg acgtcgtctc gtgcccgggc gccgagagct gcaacctcgc
     1921 cgtcaccgcg agcatgcagc tcgccgcgca cctgacccgc tttcttaaaa acccgggcaa
     1981 gtcggccgac agcgtcgagc tcgcgcgcga cgccgacatc aagatctccg ggtgtccgaa
     2041 ctcgtgcggc cagcaccacg tcgccgcgat cggcttccac ggcacggtgc gccacgccgg
     2101 cgacggccgc cagatccccg agtaccagct gcacctcggc ggcggcttca atgccgaggg
     2161 cgtcgagttc ggccgccaca tcgtcaagat cccggcccag cgcgccggcg aggcgctgct
     2221 cgccttgctc gacctgtacc gcgacaaccg cgaggccggc gaggacgcgc gcagcttcta
     2281 ccgccgcgtc ccgaaagaga aggtcgcggc tgccctgggc gagcatacga tcggcatgag
     2341 cttcgacaac ctccgcgaga ccgacctcgt cgacctcggc caggagaagc tgttcgacgt
     2401 cgcgatcggc cggggcgagt gcgcgggctg agcctcgcgc cgttcgcatg aaagagcccg
     2461 ggggtcgaga gacccacggg ctttttcgcg tgtgcgatcc gccaaatgcg aagcgcccgg
     2521 aggtcgagga ccgtccgggc gctgttcgct gtcgtgaacg actttctact tcgaggcggt
     2581 cgtcgtcggg gcggcggtct tgccggacag gtagaccttg ccctgcgaca tcgcgtaggt
     2641 gaccttcatc gtgcgcagca gcggataatt gatgtagccg ccgagctcga agctcgagcc
     2701 ctcgacgatc tgggccaggc cggcgtcggg cggggtgttg gcgagcacca ggccgccgac
     2761 gccgccgctg acggcggtct cgccgacctt gaccttctcg aggaagacca tcttgaggcc
     2821 gttggtcggg tcgtcgagct gctcgagctc gcggggcagg tggccgctct tgaggtactc
     2881 cttgcgcgcg accgccagcg cggcgccgta gctgccgccg agccaggcgt agaacttgaa
     2941 gtcggagccg tcgatcgaga cggtcgtcac cggcacgagc aggatgtgca tgtcgatcag
     3001 cagcagcggg gcctcggagg agccggccgg ggtggctgca ggggcggcgg cggcgagctc
     3061 gagcgaggca tttggaaaat cgaaggtgat cgcgccgagc ttgttcatca cctggcggcc
     3121 gagcacgacg ccgggcacct cgccgacggt gtagggcgtc aggtcctcga cgatcgcggg
     3181 cacgttggtc agcttgaggt cgccgaactt gatctccggg atcagcacga cggaggtctc
     3241 ggtgccggcg atcggggtga tcttgccgag ctccttgagc ttgagctggt cggccagcgc
     3301 cttgtggatg cgggcctcct cgcgcagctc gaagaacacg atgcgcgtga actcgccgcc
     3361 gacggtaccg ccagcgtgct tgatgccctg cgaggggtcg acctgcaggt ccgaggtgcc
     3421 cctggcgccc tcgaccttga tcagctcgcc cttgccctcg agcgggcgca tgaacgcggc
     3481 gtgcgcgagc agcagcgggc gcagcgggtt gtcgttgtcg acctgcttga acagcgcgtc
     3541 gagcgtcttg acggcgttgg cggcgtccgc gagggcgtag tagctccaca gcttggtcac
     3601 cagcgggtcg aactgggccg ggtccgcctc gagcagcttg tcctgcagct cgatcgccga
     3661 cttgtgatct cccgcggcct gatagttgcg gccgaggccg acggcggcgc cgaagttctt
     3721 ggcgtcgagc tcgagcgcct ccttgaaggc cttggtcgag gcggcataat cacccgcgcg
     3781 ccagttggcc gcgccgagga tcgcaaacgc gtccgcgttg ttcggctcgg cctcgagggc
     3841 ccgcttggcc cgctcgcgcg ccgagaacca ccgacccttc gagagatcct cgttcgccag
     3901 gccgacgagc gtgcgcgcct tggcgtcctg ctgcgcggcg gcgcgcttct tgtcctgctc
     3961 ctcgactgtg actcccttgc cgccgggcgc gttggtcccg gacttcttgc aggcgggcac
     4021 ggcgagggtg aggacgacca gagcgaggac acgggtgctg agcgacatga gttctccgga
     4081 acagggcggg ggtccgcggc gggagcatac ccgagccccc ccgccgccag caatccacct
     4141 gacccctcac gcttcggaac tctcgctccc cgacgcacgt gcgcggcggc cggcgcgggc
     4201 gagcagccag gcgcccggga cggcgagcac ggcggcgccg agcaggccca ggacgaacga
     4261 gacggcgacg aagctcgcgc ccccggcgtc gagatccgcc gccgggacga acaaactcag
     4321 ggccaggacg ctgccgacct ggtaactgcc gacctgggcc ggtccgccgg gcaactggat
     4381 gctcagcccg acgatgccga cgatcgcggc ggcctcggcg agcccgagcc cgagcccgca
     4441 ggcgtgcaac accaagtaca gctgcacgac cgtcagggcc cagtagacgg cggaccacag
     4501 cagcaagggc aggccccgcc gcaggtcggc gaggtcgcgc agggtcgccg caaactgagt
     4561 gacgaagccg gcgaggcgat ccgggaaggc cgcgccgagg cgcgcgagcc agcgggcggg
     4621 ggtccgcgcg agggcgacga ggccgaggac cagcgcgagg aacacgtacc ccatgccgcg
     4681 accgacctgc gtgacgctgg cggcgctctc gccgagcccg ggcagcagcg cggtcgcctc
     4741 ggcgacgccg acgagcacga ggccaacgac gatcaggcca tcgaccgcac gctcgctggc
     4801 gatcgcagcg atcgcctccg gtagcccgac cccgggcacc ccgccgcgcg cgagcagcag
     4861 cggccgcgac agctcgccga ggcgcagcgg caggagaatg atgacgagga acgacaccag
     4921 cccgctgccg tacagcaggt cacgcgggac gcgggcgcgg cggtccccgg tcgcctcctg
     4981 cacgcgatgg tcgagcgcgt agcgcaggcg cagcgcgcgg gccaggctgt aaggcaagtg
     5041 cagggcggcc gcggcgacga gcaggccggg tcgcggcagc gagatctcgt cgggccacag
     5101 gtcgatgcga aacgaggcga gccacagcag gccgccggcg ccgaccagcg agagcagcca
     5161 caaccacagg tcccggcgca ggcgcgcgcg cggtgcgggt tcgctcggac cgggagtcaa
     5221 ggcggcggca ccttagcatc gccgtgcatg gtgtcgaagg ctcaaaag
//
LOCUS       DASWNJ010001555         3068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_593189, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001555 DASWNJ010000000
VERSION     DASWNJ010001555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593189"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VGB85_25805"
     CDS             complement(<1..210)
                     /locus_tag="VGB85_25805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137536.1"
                     /translation="MLSAEEVLNEAVARLEQALAVERRLRDEAEALLAGLRALATAPS
                     LAAIDGKLLSCLQPVLGFEAGAVLVL"
     gene            complement(218..1414)
                     /locus_tag="VGB85_25810"
     CDS             complement(218..1414)
                     /locus_tag="VGB85_25810"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEY0137537.1"
                     /translation="MRVATSHSVEPDTSRAVEEAYAALVRELEGAPQWLLVQSSVRHP
                     AEALRRAIAGIEAGAVHGSSSCLGVMTQAGAGIDGASLALFGLRDEDGSYGVGAATPD
                     EGGDPRATAGLALQRAIAAAGRQGEVPALVWLTAAPGHEEACIAGLEAVLGPEVPVVG
                     GSAADDAVAGGWYQLTREGDFRDAVVVSALFPSGRVVSAFQSGYTPTETRGVVTRASG
                     RTLIEIDGQPAAAVYDAWTRGAIAGSIGVGGSVLASTSLFPLGRRVGDVGGVPYYRLA
                     HPATVHPDGSIDLFADIEAGDELVLMHGDVEALLTRAGRVAADARALARLTPGEVAGA
                     LVIFCAGCMLTVRDRLEQVVVGLREGLAGAPFLGSFTFGEQGCFIGGENRHGNLMISV
                     TVFART"
     gene            complement(1742..2158)
                     /locus_tag="VGB85_25815"
     CDS             complement(1742..2158)
                     /locus_tag="VGB85_25815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137538.1"
                     /translation="MALGFFVRMLGRAAILGSLLEAGCGDPPPEPDDSGGVTTVSKGG
                     PAPPKGVSTATPPAGDEGSGAPCSRWSDWNCAPTPYGNACEATCDEYIIDCNNEYGLC
                     NWAHIGDAILDSCEYTENAFDGCEGCEHGIKECQPF"
     gene            2255..>3068
                     /locus_tag="VGB85_25820"
     CDS             2255..>3068
                     /locus_tag="VGB85_25820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEY0137539.1"
                     /translation="MPVTAPAPADANKRPHQPAASGTIRAMQPLKTMIYQADGRVARV
                     TLNRPERGNGITLDMPQELAWCVEQANLDPEVRVIALSGNGRGFCGGYDLVESAEQAE
                     GMRPAEFLPGSPLDPEVFARNHDPGQTWDPMVDYAMMSRNLRGFMSLFHSDKPVVCKV
                     HGFCVAGGTDLALCSDLLVIEDTAKIGYPPARVWGVPTTALWVHRIGLEKAKRLMFTG
                     DCLSGTEAVAWGLAIECAPRDQLDAKFEALLARIARMPVNQLVMMKLMLNQSV"
BASE COUNT          458 a         1169 c         1005 g          436 t
ORIGIN      
        1 gagcaccagc accgcgcccg cctcgaagcc gagcaccggc tgcagacagc tcagcagctt
       61 gccgtcgatc gccgcgagcg acggcgccgt cgccagcgcg cgcagcccgg cgagcagcgc
      121 ctcggcctcg tcgcgcaggc gtcgctcgac cgcgagggcc tgctcgaggc gcgcgactgc
      181 ttcattgagc acttcctcgg ccgacagcat gcgtctccta cgtgcgggcg aacaccgtga
      241 ccgagatcat caggttgccg tggcgattct cgccgccgat aaagcagccc tgctcgccga
      301 aggtgaagct gccgaggaac ggcgcgccgg cgagcccctc gcgcagcccg acgacgacct
      361 gctcgaggcg gtcgcggacc gtcagcatgc acccggcgca gaagatgacg agcgcccccg
      421 cgacctcgcc cggcgtcagg cgagcgagcg cccgcgcgtc cgcggcgact cggcccgcgc
      481 gcgtcagcag ggcctcgacg tcgccgtgca tcagcaccag ctcgtcgccc gcctcgatgt
      541 cggcgaacag gtcgatcgac ccgtccgggt ggaccgtggc cgggtgcgcc agccggtagt
      601 acggcacccc gccgacgtcg ccgacgcgac gcccgagcgg gaacagcgac gtgctcgcca
      661 gcacgctccc gccgacgccg atcgaccctg cgatcgcgcc gcgcgtccac gcgtcgtaga
      721 ccgccgccgc cggctgtccg tcgatctcga tcagcgtccg tccgctcgcc cgcgtcacca
      781 ccccgcgcgt ctcggtcggc gtgtagccgc tctgaaacgc cgagaccacc cgccccgacg
      841 gaaacaacgc gctgacgacc accgcgtcgc gaaagtcgcc ctcgcgcgtc agctgatacc
      901 agccgcccgc caccgcgtca tcggccgcgc tgccgccgac gaccggcacc tcgggtccca
      961 gcaccgcctc gagccccgcg atgcaggcct cctcgtggcc cggcgccgcc gtcaaccaca
     1021 ccagcgccgg cacctcgcct tgccgccccg ccgccgcgat cgcccgctgc agcgccagcc
     1081 cggcggtcgc ccgcggatca ccaccctcgt ccggcgtcgc cgcgccgacc ccgtagctac
     1141 catcctcgtc gcgcagcccg aacagcgcca ggctcgcccc atcgatcccg gccccggcct
     1201 gcgtcatcac cccgagacac gagctgctcc cgtgcaccgc ccccgcctcg atccccgcga
     1261 tcgcccgtcg cagcgcctcc gccggatgcc gcaccgagct ctgcaccaac agccactgcg
     1321 gcgccccctc cagctcgcgg accagcgccg catacgcctc ctcgaccgca cgcgatgtat
     1381 caggctcgac gctatgactc gtggcgacgc gcatgccccg acattaccag ggatccccat
     1441 ccccgcgccg cacacccacc cgtctgcccg tggagacgaa cttcgttttc gagcatcctc
     1501 gctcgcgcca gtgctcggag atcgcgacac ggcggttgcc gacacgtcgg ttccgccccg
     1561 tggtcgcggg cggtgctgct cggcagtgcg gtttgcgggc ggtgcgttcg cggcagtgcg
     1621 gttcgcggga gtgcggttcg cgggagtgcg gttcgcgggc gaccgtcggg ctcggccaaa
     1681 cgcggggcga ggctattgtg aacttcccgg ctgaaaactc tcgaccgcgc cgccgcgctg
     1741 gtcagaacgg ctggcactcc ttgatgccat gctcgcaccc ctcgcagccg tcgaaggcgt
     1801 tctcggtgta ctcgcaggag tcgaggatcg cgtcgccgat gtgcgcccaa ttgcacagcc
     1861 catactcgtt gttgcaatcg atgatgtatt cgtcgcaggt cgcctcgcac gcgttcccgt
     1921 agggggtcgg cgcgcagttc cagtcggacc agcgcgagca cggcgcgccc gagccttcgt
     1981 cgccagcggg tggcgtcgcg gtgctcacgc ctttgggcgg cgccgggccg cccttgctga
     2041 cggtcgtaac gccaccgctg tcgtcgggct ccggtggagg gtcgccacag ccggcctcca
     2101 gcaacgagcc gaggatcgcc gctcgcccga gcatcctgac gaagaaccca agggccacgc
     2161 cgcctcgctg agcacgattc acggcagaga cggtggcacg caccgtgctg tcccgcaagc
     2221 atcaccgagc gctcgacgag gagctcgtcc cggcgtgccc gtcacggcgc ccgcccctgc
     2281 cgacgcaaac aaacgccccc accagcccgc cgcatcaggc acaatccgcg cgatgcagcc
     2341 actgaagacc atgatctatc aggcggacgg gcgggtggcc cgggtgacgc tcaatcggcc
     2401 cgagcggggc aacggcatca cgctggacat gccgcaggag ctcgcgtggt gtgtcgagca
     2461 ggccaacctc gatccggagg tgcgggtgat cgcgctgtcg ggcaacggtc gcgggttttg
     2521 tggcggctac gatctcgtcg agagcgccga gcaggccgag ggcatgcggc cggccgagtt
     2581 cttgccgggt tcgccgctcg accccgaggt cttcgcgcgc aaccacgacc ccgggcagac
     2641 ctgggatccg atggtcgact acgcgatgat gagtcggaat ttgcgcggct tcatgtcgct
     2701 ctttcacagc gacaagccgg tcgtgtgcaa ggtgcatgga ttctgcgtcg ccggcggcac
     2761 cgacctcgcg ctgtgcagcg acctcctcgt gatcgaggac accgcgaaga tcggctaccc
     2821 accggctcgg gtgtggggcg ttccgacgac ggcgctctgg gtccatcgga tcggtctcga
     2881 gaaggcgaag cgcctgatgt tcacgggcga ctgtctgtcg ggcaccgaag cggtcgcgtg
     2941 gggactcgcg atcgagtgtg caccgcgcga ccagctggat gcgaagttcg aggcgctgct
     3001 cgcgcgaatc gcacggatgc ccgtcaatca gctcgtgatg atgaagctga tgctgaacca
     3061 gagcgttc
//
LOCUS       DASWNJ010001556         3168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_593218, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001556 DASWNJ010000000
VERSION     DASWNJ010001556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593218"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VGB85_25825"
     CDS             complement(<1..823)
                     /locus_tag="VGB85_25825"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0137540.1"
                     /translation="MLSRRSFVGVAGGSALACRGGQVAAEPVREASEGSAEPPVAAES
                     IVRQVGRTGEWLPAIGMGTWETFDVGAGPNERAPLREVLRVFSAGGGRVIDSSPMYGA
                     AEQVTGDLVAETGVPTFLATKVWTRGRAAGEAQMLASMRLLRTTTIDLMQVHNLLDFD
                     THITTLRRWRDEGKIRYIGATHYQLSAFDALERVIREEQLDFVQLPYSIGVREAEKRL
                     LPAAADHGVAVLVMRPFEGGEIFKRVRGKALPEWAGELGCTSWAQIFLKFILGHPA"
     gene            complement(859..1635)
                     /locus_tag="VGB85_25830"
     CDS             complement(859..1635)
                     /locus_tag="VGB85_25830"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0137541.1"
                     /translation="MSSIPRSSAGDPARTLELLWRRTDEKAATRGPRPGRSVDEVVAA
                     AIQIADAEGLEAVTMRRVAQQLKVTAMSIYTYVPGKAELLDLMLDAVYGQMDRTDRSG
                     EPWRARVAAVAHDNRALFERHPWVTLVSTSRPPLGPGLMAKYEHELRSFAGLGLDDVE
                     VDAALTFLLGFVQASARAAAEARASQRDSAMSDAQWWAANAPLLDRVFDQEKYPTASR
                     VGAAAGAAHGAAYDPEHAYAFGLQRVLDGLGVLIEGRGGR"
     gene            1735..2124
                     /locus_tag="VGB85_25835"
     CDS             1735..2124
                     /locus_tag="VGB85_25835"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0137542.1"
                     /translation="MQITACAISLTVDDVAPSSAFFRDHLGFHEKLAADGFVSLARED
                     AGMHLVFLRRGIEVLPASYRDQTAAGVIVAFVVDDLAGEERRLRDEGVAITMPLREEP
                     WGERLFQVTDPNGVIVQLVEWATPAGA"
     gene            2169..>3168
                     /locus_tag="VGB85_25840"
     CDS             2169..>3168
                     /locus_tag="VGB85_25840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0137543.1"
                     /translation="MTRTHVFHAGLFLVLASCNGGGGDSTTTGASTTIPATTTGPDDS
                     TSTSTSTTTPTSTSTPTTGDTTTLDTTGEPGFTCTPQPLVVPVKGFFADISDASGIRV
                     DNFIKDPPVPIPINDHSRLGFADIDGDGFDDIVAHSLFPNPQKGIPFEHLVYRNKGDG
                     TFEHFSDESGLRDIQAGFFAFGDVDNDGDQDCFAGLDIELEGEHNRLLLNDGAGHFTE
                     KVDAGVDKVNLAGNAVFADFNGDANLDIFVGNGQTGYLVADQLFFGYGDGTFQDVTSG
                     FMKNAPAQPSNGSVACDYDDDGDLDIFVSTYGVSNFKGRNHLWENDGKGTMTEVAMA"
BASE COUNT          525 a         1254 c          922 g          467 t
ORIGIN      
        1 ccgcaggatg tccaagaata aattttaaaa agatctgcgc ccagctcgtg caacccaatt
       61 cgcccgccca ctcgggtaac gccttgcccc gcaccctttt aaaaatttcg ccgccctcga
      121 agggtcgcat caccagcacc gcgaccccgt gatccgcggc cgccggcagc aatcgcttct
      181 ccgcctcacg gaccccgatc gagtacggca gctgcacgaa gtcgagctgc tcctcgcgga
      241 tcacccgctc gagcgcgtcg aaggccgaca gctggtagtg cgtcgcgccg atgtaccgga
      301 tcttgccctc gtcccgccag cgccgcagcg tcgtgatgtg cgtgtcgaag tcgagcaggt
      361 tgtgcacctg catcagatcg atcgtcgtcg tccgcagcag ccgcatcgac gcgagcatct
      421 gcgcctcgcc ggccgcgcgt cctcgcgtcc acaccttcgt cgccaggaag gtcggcacac
      481 ctgtctctgc gaccaggtcc ccggtcacct gctcggccgc gccgtacatc ggcgagctgt
      541 cgatcacccg acctccgccc gccgagaaca cccgcagcac ctcgcgcagc ggcgcccgct
      601 cgttcgggcc cgcgccgaca tcaaaagtct cccacgtccc catcccgatc gccggcaacc
      661 actcaccggt gcgtccgacc tgccgcacga tcgactccgc cgccaccggc ggctccgcgg
      721 agccctcact cgcctcacgc accggctccg ccgcgacctg tcctccgcga caggcgagcg
      781 ccgacccacc cgcgaccccg acgaagctgc gccgactcag caccccgcaa cattaaacca
      841 gctacgcgac ttcgcaagct accgcccgcc gcgaccctcg atcaacacgc ctaggccatc
      901 gagcacccgc tgcagaccga acgcgtacgc gtgctccgga tcgtaggccg cgccgtgggc
      961 cgcgccggcc gccgcaccga cccgcgaggc tgtcgggtac ttctcctggt cgaacacccg
     1021 gtccagcagc ggcgcattcg cggcccacca ctgcgcgtcg ctcatcgcgc tgtctcgctg
     1081 cgacgccctc gcctccgcgg ccgcgcgcgc cgacgcctgc acgaagccga gcaggaacgt
     1141 cagcgccgcg tcgacctcga cgtcatccaa gcccagcccc gcgaagctgc gcagctcgtg
     1201 ctcgtacttc gccatcaacc ccggccccag cggcggccgt gaagtcgaca ccagcgtcac
     1261 ccacgggtgg cgctcgaaca gcgcccggtt gtcgtgcgcc accgcggcca cgcgcgcccg
     1321 ccacggctcg ccgctccggt cggtccggtc catctgcccg tacaccgcgt cgagcatcag
     1381 gtcgagcagc tccgccttgc cgggcacgta cgtgtagatc gacatcgccg tgaccttgag
     1441 ctgctgggcg acgcgccgca tcgtcaccgc ctcgagcccc tccgcgtcgg cgatctggat
     1501 cgccgccgcc accacctcgt cgacgctgcg ccccggacgt ggcccgcggg tcgccgcctt
     1561 ctcgtcggtc cggcgccaca ggagctcgag ggtccgggcc gggtcgccgg cgctgctgcg
     1621 ggggatcgat gacacggccg aaatatcgcc ttgaaattac tttttacacc gtatactgta
     1681 caacgtacag cagcgccaac cgacgcctgc tgagctttcc cagggagttt tcgaatgcag
     1741 atcaccgcat gcgccatttc gctcaccgtc gacgacgtcg ccccctccag cgccttcttc
     1801 cgggaccacc tcggcttcca cgagaagctg gcggccgacg gcttcgtctc gctcgcccgc
     1861 gaggacgccg gcatgcacct cgtgttcctg cgccgcggca tcgaggtcct gccggccagc
     1921 taccgcgacc agaccgccgc cggggtgatc gtcgccttcg tcgtcgacga cctcgccggc
     1981 gaggagcgcc gcctgcgtga cgagggcgtg gcgatcacca tgccgctgcg cgaggagccg
     2041 tggggcgagc gcctgtttca ggtgaccgac cccaacggcg tgatcgtcca gctcgtcgag
     2101 tgggccacgc cggccggcgc ctgaccggga cacttcgcaa gcgccgccag ctgcggcacc
     2161 atgggtccgt gacgcggacc catgtatttc acgccggcct gttcctcgtg ctcgccagct
     2221 gcaacggcgg cggcggcgac tcgaccacga ccggcgcctc gaccacgatc cccgcgacca
     2281 cgaccggccc cgacgactcg acgagcacgt cgacgagcac caccacgccg acgagcacga
     2341 gcacgccgac caccggcgac acgaccacgc tcgacaccac cggcgagccc ggcttcacct
     2401 gcacgccgca gccgctcgtc gtcccggtca agggcttttt cgccgacatc tccgacgcca
     2461 gcgggatccg cgtcgacaat tttattaaag atccgccggt cccgatcccg atcaacgatc
     2521 acagccggct cggcttcgcc gacatcgacg gtgacggctt cgacgacatc gtcgcgcaca
     2581 gcctgttccc caacccgcaa aagggcatcc ccttcgagca cctcgtgtat cgcaacaagg
     2641 gcgacggcac cttcgagcac ttcagcgacg agtccggcct gcgcgacatc caggccggct
     2701 tcttcgcctt cggcgacgtc gacaacgacg gcgaccagga ctgcttcgcc ggcctcgaca
     2761 tcgagctcga gggcgagcac aaccgcctgc tgctcaacga cggcgccggt cacttcacgg
     2821 agaaggtcga cgccggcgtc gacaaggtca acctcgccgg caacgccgtg ttcgccgact
     2881 tcaacggcga cgccaacctc gacatcttcg tcggcaacgg tcagaccggc tacctcgtcg
     2941 ccgaccagct gttcttcggc tacggcgacg gcaccttcca ggatgtcacc tccggcttca
     3001 tgaagaacgc ccccgcgcag ccgagcaacg gcagcgtcgc ctgcgactac gacgacgacg
     3061 gcgacctcga catcttcgtc tcgacctacg gcgtcagcaa cttcaagggt cgcaatcatc
     3121 tgtgggagaa cgacggcaag ggcacgatga cggaggtcgc catggcgc
//
LOCUS       DASWNJ010001557         7664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_487179, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001557 DASWNJ010000000
VERSION     DASWNJ010001557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7664
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487179"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VGB85_25845"
     CDS             complement(<1..272)
                     /locus_tag="VGB85_25845"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0137544.1"
                     /translation="MDVAVVGAGIVGCALASALARRGRAVVVLERAAREGTGVTSRNS
                     GVIHSGLYYPPGSVKAETCLRGQALLYAWATAQGVPHARTGKLVVAR"
     gene            340..630
                     /locus_tag="VGB85_25850"
     CDS             340..630
                     /locus_tag="VGB85_25850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137545.1"
                     /translation="MDHVSIHTFKTDDEWETIDSCWHSPFFYWQKNGLRVSPPVPLRV
                     VVWGSVVDESDEGWINMGGASAMLLQRIQARGARGQKIRVEVGEEISERQVG"
     gene            complement(750..1232)
                     /locus_tag="VGB85_25855"
     CDS             complement(750..1232)
                     /locus_tag="VGB85_25855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137546.1"
                     /translation="MAFQYQAGIYKTTKFLPGHEATVAPNMLILIRTDAEFAPASVLL
                     PVNNIHNQWRFQMPGVKIPSASFGWGESLVKLPHEGFYRLTREFSFGDTGKWLKNAIV
                     QLGYTRAGEPILFIAQRRSPLVSNDLFFSDKGVKIEMKDIDTMLEALTWYQEPDKKVN
                     "
     gene            1434..2966
                     /locus_tag="VGB85_25860"
     CDS             1434..2966
                     /locus_tag="VGB85_25860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEY0137547.1"
                     /translation="MSDSVIVVNADGPETRVALIESGILSEFYVERERERGTVGNVYK
                     GKVLRVLPGMQAAFVDIGEEKAAFLYAGDIAAPGSSGAPAPIEDDDGETVPRRPGKHG
                     DITELVRPGQEILVQVVKDPISSKGARITTYISLPGRNVVFMPTVSHIGISRRISSDR
                     ERRRLRRLVDQMRPPGAGFIVRTVAETATNGQIRADMDYLLRLWANIKVNERVHRAPV
                     LLYRDLNLMLRVVRDNLTPELSKVIVDDRQAYEKLTRFVSAFMPDCAQKIEQYSGREP
                     IFDGYGIEIELNRALERKVPLKSGGSLVFDQGEALTAIDVNTGKFVGAKGKTLEETIT
                     QTNLEAAEEIAEQLRLRNLGGLVIIDFIDMDKESNRRKVYRKLQDVLRKDRAKAHITK
                     ISEMGLVEMSRKRTRESLGRLLTAPCESCNGKGYTKSASTVTYEILREIRREAPHMTG
                     DVIHIQAAPPVAELMNGPEIAAIDHMVKRIQRGIEVQSRKDFHIEKFEIRSKNKGRPN
                     SE"
     gene            2985..3650
                     /locus_tag="VGB85_25865"
     CDS             2985..3650
                     /locus_tag="VGB85_25865"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY0137548.1"
                     /translation="MAVQARDRDDRRRGERLPINPEFAAQGPGLTFVSNLSEYGVFIN
                     TRVRLPVGTAVDLRFTVLLDDPVVIHGAGRVVHHQDEPRGMGVSFSNLAAVMQLRVID
                     AINWYRARAGAEQAFRTRELTPDELAQIDEEGEEGDESTTSLSSSDMVAESGAGYLNA
                     SIEELAPTAIAAANAAATAAAARRNQTSPSASQTQPAARSSPAPIPAPPPAPLKDDEY
                     EDF"
     gene            3692..4162
                     /locus_tag="VGB85_25870"
     CDS             3692..4162
                     /locus_tag="VGB85_25870"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEY0137549.1"
                     /translation="MSLGKRLWDMARSNITDFRSALKPDALDGLTDEERRALDEEMAD
                     TVGAKAGRTARKFKDAAEEAWEKAYEAAQARGGGGSFNGGRPSPEAERLRWYRTLELE
                     PGADHQAIRKAYRRLLVQYHPDKFAKDPEKYKAATEVTRNVTAAYDGLTALLGG"
     gene            4239..5069
                     /locus_tag="VGB85_25875"
     CDS             4239..5069
                     /locus_tag="VGB85_25875"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0137550.1"
                     /translation="MPRSSAASKTKPPRTLAAQADRHALYERAVQDPEGDVKFFARTY
                     RKIRGHDPVSMREDFCGTAALCEAWARSSPERTALGVDLSQDTMNWGLEHHIVPAGRE
                     IASRITLLCEDVRNVKKPKVDLTCALNFSYYCFKERADLLDYFRAVHAGLNRDGVFLL
                     DMLGGTEAIVEGTTDNDHTDFIYRWEQAKFDAFTHDFLCYIHFLFPDGSKIYRAFTYD
                     WRLWMAPELRDILLEAGFSKVHFYWEKTDAKGEGTGVFYEPDYVENQEIWWTYLAAER
                     "
     gene            5086..5997
                     /locus_tag="VGB85_25880"
     CDS             5086..5997
                     /locus_tag="VGB85_25880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137551.1"
                     /translation="MPKQPAKTTNKKTPAQKSGKKQSGKKTTAKKAARPSLAERADKH
                     ALYQVSVQSPGVDIEFFEAEFEKYRGRKPMRLREDFCGTAHLSTEWCKTDPGRTALGV
                     DLDGATLEWGRVHNLEPAGGDVARRVKLAQADVRETRFAKVDLTLAMNFSYCVFQTRE
                     DLQSYFNAAHAGLIRDGVLALEVYGGTESMSTLEEERQVRGGATYVWEQEKFSPIDHH
                     TVCHISWRFRDGSEMKRAFTYDWRLWTLPELKELLLAAGFKAVKFYFERVDGDEDDEF
                     LTGTGDFIEQTEIDNQEAWLAYVVALK"
     gene            6177..7301
                     /locus_tag="VGB85_25885"
     CDS             6177..7301
                     /locus_tag="VGB85_25885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137552.1"
                     /translation="MDIAISVTLGLLFMVASLVATCLRMICWRSGGATAGGPLLLRLR
                     GIFELIYAAIYVLMMFEMLPRLWAYQVELPPRTVFHVAIGFVLGALLLLQLIASAARD
                     LRAWQPVLSAMVFVNTVLLCGLSVPFALHEYGLARGQAGGSVYSDESREALARALPMA
                     GLPEGARLGELTAVDSLRRGRNVLAGKCVVCHDLKTVLVQPRKPADWWKTVKRMAEKP
                     TFSEPLGEVEQWEVTAYLIAIANDVSAEDLAQHEETAERRATAIAVATKRGSNFGADR
                     VLYEKICSECHPLADIEAHAPRSPEEVAAVIERMIVDNGLVASKEEVELVYGFMINLY
                     VADAAGEKAPMLTGPHAPVVPARLTEVKPAKIMKRPAKKK"
     gene            complement(7302..>7664)
                     /locus_tag="VGB85_25890"
     CDS             complement(7302..>7664)
                     /locus_tag="VGB85_25890"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEY0137553.1"
                     /translation="EGQIDHLGVFPWSREDGTPSALLPGRVDPEEAEERRLALMELQE
                     EIRQARQEAMIGREIEVMVEGVSEESEFLLDGRHEGQAPGIDGKVILTDGSAEPGDFV
                     RARVTQAEAHDLVATLEL"
BASE COUNT         1315 a         2638 c         2603 g         1108 t
ORIGIN      
        1 cgcgcgacca ccagcttgcc cgtgcgcgcg tgcgggaccc cctgcgcggt cgcccacgcg
       61 tacagcagcg cctgtccccg aagacaggtc tccgccttga ccgagccggg gggatagtac
      121 agcccgctgt ggatcacgcc gctgttgcgg ctggtgacgc cggtgccctc gcgtgccgct
      181 cgctcgagca cgaccaccgc gcgaccgcga cgggccagcg ccgacgcgag cgcgcagccg
      241 acgatgcccg cgccgacgac cgcgacgtcc atgggggcac tctagcgcga cgcgggccgg
      301 cttgcccggg ccccggctgc gacgtaaaac ggccgcgcga tggaccacgt cagcatccac
      361 accttcaaga ccgacgacga gtgggagacc atcgacagct gctggcacag cccgttcttc
      421 tactggcaaa aaaacggctt gcgggtcagc ccgccggtgc cgttgcgggt cgtcgtgtgg
      481 ggctcggtgg tcgacgagtc ggacgagggc tggatcaaca tgggcggcgc gagcgccatg
      541 ctgctgcaac ggatccaggc caggggcgcg cgcgggcaga agatccgcgt cgaggtcggt
      601 gaggagatct ccgagcggca ggtcggctga atcgacggcc cgggctcccg aaggccctta
      661 aacgccgcga gcctcgaccg tcgcgtggcg actgtcgagg ctgcggaggg acggcccgaa
      721 tgaaccggag agcaccgagg ctgctggcct cagttcacct tcttgtcggg ctcctgatac
      781 caggtgaggg cctcgagcat cgtgtcgatg tccttcatct cgatcttgac gcccttgtcg
      841 ctgaagaaca ggtcgttgct gacgagcggg ctgcggcgct gggcgatgaa caggatcggc
      901 tcaccggcgc gggtgtagcc gagctggacg atcgcgttct tgagccactt gccggtgtcc
      961 ccgaagctga actcgcgggt gagccggtaa aagccctcgt gcggcagctt gaccagcgac
     1021 tcgccccatc cgaacgacgc cgacgggatc ttcaccccgg gcatctggaa gcgccactgg
     1081 ttgtggatgt tgttgacggg cagcaggacg gaggccggcg cgaattcggc gtccgtacgg
     1141 atcaggatca acatgttggg ggcgacggta gcttcatgcc cgggcagaaa cttcgtcgtc
     1201 ttgtagatcc ccgcttggta ctgaaaggcc atacggcgac gaacagtaca gaaagccgtc
     1261 cctccaggaa aggccaaagc gtcgtttgtc cgcgaaccga gcgcaggtgc cgacccgctc
     1321 cgtttatccc gcgcgggcgg tggccttttt gcccgggccc gctccgaagc gcgagctccc
     1381 ctgtcatccc ttgtcattgg cgcgcccggt tgtgctagga aggcacggcc gccgtgtctg
     1441 attccgtcat tgtagtcaac gcagatggcc cggagacccg ggtcgccctc attgagagcg
     1501 ggatcttgtc cgagttctac gtcgagcgcg agcgcgagcg tgggaccgtg ggcaacgtct
     1561 acaagggcaa ggtgctgcgg gtgttgccgg gcatgcaggc cgccttcgtc gacatcggcg
     1621 aggagaaggc cgccttcctg tacgccggcg acatcgccgc tcctggttca tcgggtgcgc
     1681 ccgcgccgat cgaggacgac gacggcgaga ccgtcccgcg ccgccccggc aagcacggcg
     1741 acatcaccga gctggtccgt cccggccagg agatcctcgt tcaggtcgtc aaggacccga
     1801 tctccagcaa gggcgcgcgg atcaccacgt acatctcgct gccgggtcgc aacgtcgtgt
     1861 tcatgccgac ggtgagccac atcggcatct cgcggaggat ctcgagcgac cgcgagcgcc
     1921 gtcgcctgcg tcgcctcgtc gaccagatgc gtccgcccgg cgccggcttc atcgtccgca
     1981 cggtcgccga gacggcgacc aacggccaga tccgggccga catggactac ctgctgcgcc
     2041 tgtgggccaa catcaaggtc aacgagcgcg tgcatcgggc cccggtgctg ctgtaccgcg
     2101 acctcaacct gatgctgcgg gtcgtgcgtg acaatctgac gcccgagctg agcaaggtga
     2161 tcgtcgacga ccgccaggcg tacgagaagc tgacgcgctt cgtctcggcc ttcatgccgg
     2221 actgcgcgca gaagatcgag cagtacagcg ggcgcgagcc gatcttcgac ggctacggca
     2281 tcgagatcga gctcaaccgc gcgctcgagc gcaaggtccc gctcaagagc ggcggctcgc
     2341 tggtcttcga tcagggcgag gccctgaccg cgatcgacgt caacaccggc aagttcgtcg
     2401 gtgccaaggg caagacgctc gaggagacga tcacccagac caatctcgag gcggcggagg
     2461 agatcgccga gcagctgcgc ctgcgcaacc tcggcggcct ggtgatcatc gacttcatcg
     2521 acatggacaa ggagtccaac cggcgcaagg tctaccgcaa gctgcaggat gtcctgcgca
     2581 aggaccgcgc caaggcccac atcaccaaga tctcggagat gggcctcgtc gagatgagcc
     2641 gcaagcggac ccgggagagc ctcggccgcc tgctgaccgc cccctgcgag tcgtgcaacg
     2701 gcaagggtta cacgaagtcc gccagcaccg tgacgtacga gatcctgcgc gaaattcgcc
     2761 gcgaggcgcc ccacatgacc ggggacgtca tccacatcca ggcggcgccg ccggtcgcgg
     2821 agctgatgaa cggcccggag atcgccgcga tcgaccacat ggtcaagcgg atccagcggg
     2881 gcatcgaggt ccagagccgc aaggatttcc acatcgagaa attcgagata aggtcgaaga
     2941 acaagggccg tccgaacagc gagtaggcag gggagaccgg gaccatggca gtgcaggcgc
     3001 gagacaggga tgaccggcgt cgcggcgaac gcctcccgat caacccagaa ttcgccgccc
     3061 agggtcccgg gctgaccttc gtcagcaacc tcagcgagta cggcgtgttc atcaacacgc
     3121 gcgtgcgcct gccggtcggc accgcggtcg acctgcgctt caccgtgctg ctcgacgacc
     3181 cggtcgtcat ccacggggcc ggccgcgtcg tgcatcacca ggacgagccg cgcggcatgg
     3241 gcgtctcctt cagcaacctc gccgcggtga tgcagctgcg cgtgatcgat gcgatcaact
     3301 ggtaccgggc gcgcgccggc gccgagcagg cgtttcgcac gcgcgagctg acccccgacg
     3361 agctggccca gatcgacgag gagggcgagg agggcgacga gtcgacgacc agcctcagca
     3421 gcagcgacat ggtcgccgag agcggcgccg gctacctcaa cgccagcatc gaggagctcg
     3481 cgccgaccgc gatcgcggcc gcgaacgccg ccgccaccgc agcggcggcc cgccgcaacc
     3541 agacctcgcc atcggcgagc cagacgcagc ccgccgcccg gtcctcgccc gcgcccatcc
     3601 ccgcgccgcc gcccgccccg ctcaaagacg acgagtacga ggacttctag gcccctttgc
     3661 aggggtcgcc caaaagggcc atattggggc ggtgagcctc ggtaagcgcc tctgggacat
     3721 ggcccgttcg aacatcacgg actttcgctc cgccctcaag ccggacgcgc tggacgggct
     3781 gacggacgag gagcggcgcg cgctcgacga ggagatggcc gacaccgtcg gtgccaaggc
     3841 aggacgtact gcgcgcaagt tcaaggacgc cgccgaggag gcgtgggaga aggcctacga
     3901 ggccgcccag gcgcgcggcg gcgggggctc gttcaacggc ggtcgcccct cgccggaggc
     3961 cgagcgcctg cgctggtatc ggaccctcga gctcgagccc ggcgccgacc accaggcgat
     4021 ccgcaaggcc taccggcgct tgctggtcca gtaccacccg gacaagttcg ccaaggaccc
     4081 cgagaagtac aaggcggcga ccgaggtcac ccgcaacgtc accgccgcgt acgacggcct
     4141 gacggccctg ctcgggggat gaggctcggg ggatggcccg cgcggtagca gtggaccgcg
     4201 cgaggcgacc tcgcgtattt tgccgcgcgt aggtcccctt gccccgcagc agcgccgcct
     4261 cgaaaaccaa gcctccccgc accctcgccg cgcaggcgga ccgccacgcg ctctacgagc
     4321 gggcggtgca agaccccgag ggcgacgtca agttcttcgc ccgaacgtat cgcaagatcc
     4381 gcgggcacga tccggtcagc atgcgcgagg acttttgcgg gaccgcggct ctgtgcgagg
     4441 cgtgggcccg cagctcgccc gagcgcaccg cgctgggcgt cgacctctcg caggacacga
     4501 tgaactgggg cctcgagcac cacatcgtac ccgcgggccg cgagatcgcg agccgcatca
     4561 cgcttttgtg cgaggacgtt cggaacgtca agaagcccaa ggtcgacctg acctgcgcgc
     4621 tcaacttttc atactactgc ttcaaggagc gcgccgacct gctggactac ttccgggcgg
     4681 tgcacgcggg gctcaaccgc gacggcgtgt tcctgctcga catgctcggc ggcaccgagg
     4741 cgatcgtcga gggcacgacc gacaacgacc acaccgactt catctatcgc tgggagcagg
     4801 ccaagttcga cgccttcacc cacgacttcc tctgttacat ccacttcctg ttccccgacg
     4861 gctcgaagat ctaccgcgcc ttcacctacg actggcgcct gtggatggcc cccgagctaa
     4921 gggacatcct gctcgaggcc ggtttttcga aggtccactt ctactgggag aagaccgacg
     4981 ccaagggcga gggcaccggc gtgttctacg agcccgacta cgtggaaaac caggagatct
     5041 ggtggaccta cctcgccgcc gagcgctgac ccccgcgtga acgccatgcc caaacagccc
     5101 gccaagacca cgaacaagaa gacccccgcg cagaagtcgg ggaagaagca gtccgggaag
     5161 aagacgaccg ccaagaaggc cgctcgcccg agcctcgccg agcgcgccga caagcacgcg
     5221 ctctaccagg tctccgtgca gtcgcccggc gtcgacatcg agttcttcga ggccgagttc
     5281 gagaagtacc gcggccgcaa accaatgcgc ctgcgcgagg acttctgcgg caccgcgcac
     5341 ctgtcgaccg agtggtgcaa gaccgacccg ggccgcacgg ccctcggcgt cgacctcgac
     5401 ggcgcgacgc tcgagtgggg gcgcgtacac aacctcgagc ccgccggtgg ggacgtcgcg
     5461 cgccgcgtca agctggccca ggccgacgtg cgagagaccc gcttcgccaa ggtcgacctg
     5521 acgctggcga tgaacttcag ctactgcgtg ttccagaccc gcgaggacct ccagagctac
     5581 ttcaacgccg cccacgccgg cctgatccgc gacggcgtgc tcgcgctcga ggtctacggc
     5641 ggcaccgaga gcatgagcac gctcgaggag gagcggcagg tccgcggcgg cgcgacctat
     5701 gtctgggagc aggagaagtt ctcgccgatc gatcaccaca cggtgtgtca catcagctgg
     5761 cgctttcgcg acggcagcga gatgaagcgg gccttcacct acgactggcg cctgtggacc
     5821 ctgcccgagc tcaaggagct gctgctcgcc gccggcttca aggcggtcaa gttctacttc
     5881 gagcgcgtcg acggcgatga ggacgacgag ttcctgaccg gcactggcga cttcatcgag
     5941 cagacggaga tcgacaatca ggaggcctgg ctggcctacg tcgtcgcgct caagtgacgc
     6001 gtcgaggccc cgcgaccccg atcgcgccgg cgttatctcc gttccgcgag cacgtgtcgc
     6061 cggcaggccg cgcgcgcaag caagcacgcg cggtagcacg cagattcgct agagggagga
     6121 cgtgttagcg tcggatctcg ggcgccgatt gccggcgccg gagatggacg ttcggcatgg
     6181 acatcgcgat cagcgtgaca ctggggctgc tgttcatggt cgcgtcgctg gtcgcgacct
     6241 gcctgcggat gatctgctgg cgctcgggcg gtgccacggc cggggggccg ctgctgctgc
     6301 gcctgcgcgg gatcttcgag ctgatctacg ccgcgatcta cgtgctgatg atgttcgaga
     6361 tgctgccgcg gctgtgggcc tatcaggtcg agctgccgcc gcgcaccgtc tttcacgtgg
     6421 cgatcggctt cgtgctcggc gccctgctgc tgctccagct gatcgccagc gccgcccgcg
     6481 acctgcgggc ctggcagccg gtcctcagcg ccatggtgtt cgtcaacacc gtgctgctgt
     6541 gcgggctctc ggtcccgttc gcgctgcacg agtacgggct cgcccgcggc caggccggcg
     6601 gcagcgtgta cagcgacgag agccgcgagg ccctcgcgcg cgcgctgccg atggcgggcc
     6661 tgcccgaggg cgccaggctc ggggagctga ccgcggtcga ctcgctgcgg cgcggacgca
     6721 acgtgctcgc gggcaagtgc gtcgtctgtc acgacctcaa gaccgtgctg gtccagccgc
     6781 gcaagccggc ggactggtgg aagacggtca agcgcatggc ggagaagccg accttcagcg
     6841 agccgctcgg cgaggtcgag cagtgggagg tgaccgcgta tctgatcgcg atcgccaacg
     6901 atgtcagcgc cgaggacctc gcgcagcacg aggagaccgc cgagcgccgg gcgacggcga
     6961 tcgccgtggc gaccaagcgc gggagcaact tcggggccga tcgcgtcctc tacgagaaga
     7021 tctgctccga gtgccacccg ctggcggaca tcgaggccca cgcgccgcgc agccccgagg
     7081 aggtcgccgc cgtcatcgag cgcatgatcg tcgacaacgg gctcgtcgcc tccaaggagg
     7141 aggtcgagct cgtctacggc ttcatgatca atctctacgt cgccgacgcc gcgggcgaga
     7201 aggcgccgat gctgaccggc ccgcacgcgc ccgtggtccc ggcgcggctg accgaggtca
     7261 agccggccaa gatcatgaag cggccggcca agaagaagtg atcacagctc gagcgtggcg
     7321 acgaggtcgt gcgcctcggc ctgcgtgacg cgggcgcgga cgaagtcgcc gggctcggcg
     7381 ctgccatcgg tgaggatgac cttgccgtcg atgccggggg cctggccctc gtggcggccg
     7441 tcgagcagga actcgctctc ctcgctgacg ccctcgacca tgacctcgat ctcgcggccg
     7501 atcatcgcct cctgccgggc ctgacggatc tcctcttgca gctccatcag cgcgaggcgg
     7561 cgctcctcgg cctcctcggg gtcgacgcgg ccgggcagca gcgccgaagg cgtgccgtcc
     7621 tcgcgcgacc acgggaacac gccgaggtga tcgatctggc cctc
//
LOCUS       DASWNJ010001558         1771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_167448, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001558 DASWNJ010000000
VERSION     DASWNJ010001558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167448"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1617)
                     /gene="metG"
                     /locus_tag="VGB85_25895"
     CDS             complement(<1..1617)
                     /gene="metG"
                     /locus_tag="VGB85_25895"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF001100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEY0137554.1"
                     /translation="MSGRVLVTAALPYANGSIHLGHMLEAIQTDVYVRARKLAGDDVI
                     FMWAADTHGTPIELRARKEGITPEALIERAHAEHAQVFKDFGIGSDIYYTTHSEETRR
                     HAEAIFAAMRAAGHIHTRATNQLYCPNDAMFLPDRFVRGQCPKCGALDQYGDSCEVCG
                     STYAPADLKDPHCSICGTTPVLRESEHLFVALGEHEAFLREWLQAPEAGGRTRLQSTV
                     RNYVMRWIEDGLRDWDISRDAPYFGFAIPGYEGKKFFYVWFDAPIGYIGATEKWCADN
                     GRDASEYWGGDSEIVHVIGKDIVYFHTLFWPAMLHTAGYTTPSRVQVHGWLTVNGEKM
                     SKSRGTFILARTYLDQLPPDYLRYYFAAKLGTEQDDIDLSLEDFATRVRADLVNKAAN
                     LASRCVKFVHQRLGGRLGPPAEDALPLLARAAERLQEVPGLYREFESAKALRLAMEIA
                     EDFNLFITESAPWKLATSDPERARAICSAAVHAGKIVAAILKPVLPAWAEKTERMLRL
                     PQPLDFNNAADRLPDDHTIDAYETLAEPIDP"
BASE COUNT          268 a          615 c          599 g          289 t
ORIGIN      
        1 cgggtcgatc ggctccgcga gcgtctcgta ggcgtcgatc gtgtgatcgt ccggcagccg
       61 gtccgcggca ttattaaaat cgagcggctg cgggagccgc agcatgcgct cggtcttctc
      121 ggcccacgcc ggcagcaccg gcttcaagat cgccgccacg atcttcccgg cgtgcaccgc
      181 cgccgagcag atcgcacgcg ctcgctccgg gtcgctcgtc gccagcttcc acggggccga
      241 ctcggtgatg aacaaattga aatcctccgc gatctccatc gccaggcgca gcgccttcgc
      301 cgactcgaac tcgcggtaca gccccggcac ctcttgcaaa cgctcggccg cccgcgcgag
      361 caagggaagc gcgtcctcgg ccggcggacc gagccgcccg ccgagccgct ggtgcacgaa
      421 cttcacgcag cggctcgcca ggttggccgc cttgttgacg aggtcggcgc gcacgcgcgt
      481 cgcgaagtcc tcgagcgaca ggtcgatgtc gtcctgctcg gtgcccagct tggcggcgaa
      541 gtagtagcgc aggtagtccg gcggcagctg gtcgaggtag gtgcgcgcga ggatgaaggt
      601 cccccgcgac ttcgacatct tctcgccgtt gaccgtcagc cagccgtgca cctgcacgcg
      661 cgagggcgtc gtgtagccgg ccgtatgcag catcgccggc cagaacagcg tgtggaagta
      721 gacgatgtcc ttgccgatga cgtgcacgat ctccgagtcg ccgccccagt actcgctcgc
      781 gtcgcggccg ttgtcggcgc accacttctc ggtcgcgccg atgtagccga tcggcgcgtc
      841 gaaccacacg tagaagaact tcttgccctc gtagccgggg atcgcaaacc cgaagtaggg
      901 cgcgtcgcgc gagatgtccc agtcgcgcag gccgtcctcg atccagcgca tcacgtaatt
      961 gcggaccgtc gactgcaagc gcgtgcgacc gccggcctcc ggcgcctgca gccactcgcg
     1021 caggaacgcc tcgtgctcgc cgagcgccac gaacaagtgc tccgactcgc gcagcaccgg
     1081 cgtcgtcccg cagatgctgc agtgcgggtc cttgaggtcc gcaggcgcgt aggtcgagcc
     1141 gcacacctcg cagctgtcgc cgtactggtc gagcgcgccg cacttcgggc actgcccgcg
     1201 cacgaagcgg tccggcagga acatcgcatc gttgggacag tacagctggt tggtcgcccg
     1261 cgtgtggatg tgcccggccg cgcgcatcgc cgcgaagatc gcctccgcgt gacggcgcgt
     1321 ctcttccgag tgggtcgtgt aataaatgtc ggagccgatg ccgaagtctt tgaagacctg
     1381 ggcgtgctcg gcgtgggcgc gctcgatcaa cgcctcgggc gtgatgccct ccttgcgcgc
     1441 gcgcagctcg atcggcgtgc cgtgggtgtc ggccgcccac atgaagatga cgtcatcgcc
     1501 ggcgagcttg cgggcccgca cgtagacgtc cgtctggatc gcctcgagca tgtgcccgag
     1561 gtggatcgat ccgttggcgt aggggagcgc tgcggtgacg aggacgcggc cagacatcgc
     1621 cgcgcatata acacttgcga gcccgcccgg taaaggcggg ccgcccgtct cagaccgcag
     1681 agtcgcgggc gatcgcctcg cggctggctg cgatctgcgc ctcgaggtcc tccagctgct
     1741 cgagcagcga cttcgcggtg gtgcagcgcc g
//
LOCUS       DASWNJ010001559         1708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_132165, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001559 DASWNJ010000000
VERSION     DASWNJ010001559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132165"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VGB85_25900"
     CDS             complement(<1..553)
                     /locus_tag="VGB85_25900"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY0137555.1"
                     /translation="MQLNPEQQAAVEHRGSPLLVLAGAGTGKTRVITHRVAKLLADGV
                     PAWRILAVTFTNKAAGEMRERIHQMCEGVPEVDRLWVGTFHSVCARILRRYGEGVGLT
                     RNFAIYDTDDQMRIMKRALKELNIDDRQLSPRAVLTHLDRAKNRGLPISKLEQLGLDE
                     PVNSLSKKAGLRYEQLVRAADAAD"
     gene            complement(573..1484)
                     /locus_tag="VGB85_25905"
     CDS             complement(573..1484)
                     /locus_tag="VGB85_25905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137556.1"
                     /translation="MLVDPVYPSHASHASSAAQGKRGRPSNRYAVELDGKQCGWLTDL
                     AGAAVMGELGSNAGRFEELTIRCWPAMPRGFFQWVLRSFAPEPTGSDGLLVLADDSDR
                     ELSRLRWTDGVVTEFATTAIDASSPDNAKLALKFTPRLSQRGEVLGPEVSPAAADTRR
                     PLPAPMPELQIDGLEEVCCHIKRIEGLVIRQRIRRTQGDSGMGIVCEREAPGIYMSPL
                     VLTLPESKGQGFRRWLEACEAGKSNGRTATLMLGAPSVGFTVALQGVRPVRIDDTPGM
                     DRDDPRRRDLRVELRARDVVFALAPGK"
BASE COUNT          235 a          582 c          625 g          266 t
ORIGIN      
        1 agtcggcggc gtcggcggcc cgcacgagct gctcgtagcg caggccggcc ttcttgctga
       61 gcgagttgac gggctcgtcg aggccgagct gctcgagctt cgagatcggc aggccgcggt
      121 tcttggcgcg gtcgaggtgg gtcaacacgg cgcgcggcga gagctggcgg tcgtcgatgt
      181 tgagctcctt gagcgcgcgc ttcatgatgc gcatctggtc gtcggtgtcg tagatcgcga
      241 agttgcgggt caggccgacg ccctccccgt agcggcggag gatgcgggcg cagaccgagt
      301 ggaaggtgcc gacccacaga cgatcgacct cgggcacgcc ctcgcacatc tgatggatgc
      361 gctcgcgcat ctcgccggcg gccttgttgg tgaaggtgac cgcgaggatc cgccaggcag
      421 gcacgccgtc cgcgaggagc ttggcgacgc ggtgggtgat cacccgcgtc ttgccggtgc
      481 ccgcgcccgc caggaccagc agcggcgagc cgcggtgctc gacggccgcc tgttgttcag
      541 ggttgagctg cacgcgcgga gtttgccgcg cctcacttgc ccggcgccag cgcgaagacg
      601 acgtcccggg cccggagctc gacgcgaaga tcacggcggc gtgggtcgtc gcggtccatc
      661 ccgggcgtgt cgtcgatgcg gaccgggcgc accccctgca gggcgaccgt gaagccgacg
      721 ctcggcgcgc cgagcatcag ggtcgcggtg cggccgttgc tcttgccggc ctcacaggcc
      781 tcgagccagc ggcgaaagcc ctgccccttc gactccggca gcgtcaatac cagcgggctc
      841 atgtagatcc ccggcgcctc gcgctcacac acgatgccca taccggagtc gccctgggtc
      901 cgccggatcc gctggcgaat gacgaggccc tcgatgcgct tgatgtgaca gcacacctcc
      961 tcgaggccgt cgatctgcag ctcgggcatc ggcgctggca acgggcgtcg cgtgtcggcg
     1021 gccgccggcg agacctccgg gccgagcacc tcgccgcgct gcgacagccg cggcgtgaac
     1081 ttcagcgcga gcttggcgtt gtcgggcgag ctggcgtcga tcgccgtggt cgcgaactcg
     1141 gtgacgacgc cgtcggtcca gcgcaggcgg ctgagctcgc ggtcactgtc gtcggcgagg
     1201 accagcagac cgtcgctgcc ggtcggctcg ggcgcgaacg accgcaacac ccactgaaaa
     1261 aatccgcgcg gcatcgcggg ccagcagcgg atcgtcagct cctcgaagcg gcccgcgttc
     1321 gacccgagct cgcccatcac cgccgcaccg gcgaggtcgg tcagccagcc gcactgcttc
     1381 ccgtcgagct cgaccgcgta gcggttcgag ggccgcccgc gcttgccctg cgcggccgag
     1441 gacgcgtggg acgcatggga tggatagacg ggatcgacga gcacggcgac tccatggatg
     1501 ccttaacccg ggctcggcct tctggctttt tttttagggg tcgcgtgacc ccgctccaga
     1561 ttggccccag gcgagagttc acggccgatt ggaccgccgt cattcgcccc gggggaaggt
     1621 gtcgctggca gcgctggggt tggtcatcga gtcgtccggg gctgccgcgt gggcggggat
     1681 gcgcacggtg aaggtggagc ccttgccc
//
LOCUS       DASWNJ010001560         1746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_339512, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001560 DASWNJ010000000
VERSION     DASWNJ010001560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339512"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1746
                     /locus_tag="VGB85_25910"
     CDS             <1..>1746
                     /locus_tag="VGB85_25910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137557.1"
                     /translation="PPAGSRVPAWLHAAVVRGLARDPAARWPSMLALLAELTRDHHAL
                     RRRWTLAAVITGLGVVPLGLGVAAGVEHLEHGARKAACELRGAEVSDMYDLGTRAGLL
                     RRLGPSPTAAEIEGLGDAASTIEQRAVRLRDERVALCMREPEGYVHPQHLLEQHARID
                     RCFAKSSDDLRTILAVAGQVDPETAGLVMQTATFGPEDCREAATLHGKAPEYPEDPAA
                     RSRWQAIYAEVVRGRLELAAGRPGAAESILVAALAEADAMHATALRIDVARALTRAHF
                     AAGHRRAAWGVFEAALAASPEFIEDRGGETLDPLALQLALGAATGKPRVTTALLMLDG
                     SKDKTSIAASEGLTGVLQRGVSPLWRGSFPSKMGSWIYGMALPGLGATAQLRLGVGDF
                     EAAEEYARNTLAISSFHPRDPGRAADLLLVLAQAQQGLDRHAQAGASLQRALVEANAD
                     AAARNRLPQIHLALADLALARGALDVAERELSAAESLAAEAEADDDRLIPALTLALGR
                     LHAARGDHDGAEQQLQRARKALLASHGAARRMLVTVEVELGRLALARADLASAASALD
                     TAIRLNEALVGATHVS"
BASE COUNT          202 a          738 c          603 g          203 t
ORIGIN      
        1 ccaccggcgg gctcgcgcgt gcccgcgtgg ctgcacgcgg cggtggtccg cgggctcgcc
       61 cgcgaccccg cggcgcgctg gccctcgatg ctcgcgctgc tcgccgagct cacccgcgac
      121 caccacgctc tccggcgccg ctggaccctc gccgcggtga tcaccggcct cggcgtcgtc
      181 ccgctcgggc tcggcgtcgc cgcgggcgtc gagcacctcg agcacggcgc gcgcaaggcc
      241 gcctgcgagc tgcgcggcgc cgaggtctcg gacatgtacg acctcggcac ccgcgctggc
      301 ctgctgcgca ggctcggccc gagcccgacc gccgccgaga tcgagggcct cggcgacgcc
      361 gcctcgacga tcgagcagcg cgcggtccgc ctgcgcgacg agcgggtcgc cctgtgcatg
      421 cgcgagcccg agggctacgt gcacccgcaa cacctgctcg agcagcacgc tcgcatcgac
      481 cgctgcttcg cgaagtccag tgacgacctg cgcacgatcc tggcggtcgc cgggcaggtc
      541 gatcccgaga ccgccgggct ggtcatgcag accgcgacct ttggccccga ggactgccgc
      601 gaggccgcga ccctgcatgg caaggccccc gagtatcccg aggaccccgc ggcccgcagc
      661 cgctggcaag cgatctacgc ggaggtcgtg cgcggccgcc tcgagctcgc cgccggccgt
      721 cccggcgcgg ccgagtcgat cctcgtcgcc gcgctcgccg aggccgacgc gatgcacgcg
      781 accgcgctgc gcatcgacgt cgcccgcgcc ctgacccgcg cccacttcgc cgccgggcac
      841 cgccgggccg cctggggcgt cttcgaggcc gcgctcgcgg cctcgcccga gttcatcgag
      901 gaccgcggcg gcgagacgct cgacccgctc gcgctccagc tcgccctcgg cgccgcgacc
      961 ggcaagccgc gcgtcaccac ggcgctgttg atgctcgacg gctcgaagga caagacgtcg
     1021 atcgccgcca gcgagggcct caccggcgtg ctgcagcgcg gcgtctcgcc gctgtggcgc
     1081 ggctccttcc cgagcaagat gggctcgtgg atctacggca tggcgctgcc gggcctcggc
     1141 gcgaccgccc agctgcgcct cggcgtcggc gacttcgagg ccgccgagga gtacgcccgc
     1201 aacacgctcg ccatctccag ctttcacccc cgcgaccccg gacgcgcggc cgacctgctg
     1261 ctcgtgctcg cccaggccca gcagggcctc gatcgccacg cgcaggccgg agcctcgctg
     1321 cagcgcgcgc tcgtcgaggc gaacgccgac gcggccgccc gcaaccgcct gccgcagatc
     1381 cacctcgcgc tcgccgacct cgcgctcgcc cgcggtgcgc tcgacgtcgc cgaacgcgag
     1441 ctctccgcgg ccgagtcgct cgccgccgag gccgaggccg acgacgaccg cctgatcccc
     1501 gcgctgaccc tcgcgctcgg ccgcctgcac gccgcgcgcg gcgatcacga cggcgccgag
     1561 cagcagctgc aacgcgcccg caaggcgctg ctggcgagcc acggcgcggc ccgccgcatg
     1621 ctcgtcactg tcgaggtcga gctcggccgc ctggccctgg cccgcgccga cctggcgtcc
     1681 gccgcgtccg cgctcgacac cgcgatccgg ctcaacgagg cgctcgtcgg cgcgacgcac
     1741 gtctcg
//
LOCUS       DASWNJ010001561         3565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_132185, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001561 DASWNJ010000000
VERSION     DASWNJ010001561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132185"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1638
                     /locus_tag="VGB85_25915"
     CDS             <1..1638
                     /locus_tag="VGB85_25915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137558.1"
                     /translation="PCLEAAGFDPAVFGRFKGVIAYTGKKGGDLRKARALRAKLEKDM
                     KKAGLDPADPELAKDHPKLGGSYATWKDLQTRIEVIDHAQRRLRCEKMFVSAEGVGKY
                     ETGVFDTATTHALAAFEKKHAIKGWGHFTQDNLAALALSQQESMHRRLLRVVEERTVS
                     GASILEDGSAARWKPKFRWTDSAGKEHALRDVVGEAQAAMVAALDLATPESARKQLDR
                     LSDLGGGNFDNLLVAVRLPPPPAYYAEDMTFDVVIDRGDVWYDFPFDAEGNKLVQPRE
                     RYPHFTIYAVVGEQKIPLVHWRTTIGSWRSEMYNGAEHYKYKDSDVGDRVWKEIVAAP
                     VWIPPTSTPPRELVRRKWKDGKVRTVVNYGEFGPSYTSAYGLVAAYHIKEIKDAEGNW
                     KADFDNGIRTHGSVDYMSILRRYSHGCHRLYNMSAVRLFSFVLAHHEFTREGQVKLGF
                     AREFAHEGKPFKISLKTRGYRYDLATPIAVHVLEGRVRGNRKTPVEGYVPKPTPALAP
                     GDDLSPDSPPDATATDVTPVTPMPMPMNPPAPAPAPL"
     gene            2011..2859
                     /locus_tag="VGB85_25920"
     CDS             2011..2859
                     /locus_tag="VGB85_25920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137559.1"
                     /translation="MQPNLASGFDDSFRRHLAVEQRRAVLDTIARELTPDTTLGDIVD
                     AAQDLGWAENLGELSLAELALALLGEPPTATAPARAPAATHAPDEDEEDELEADQETD
                     ADEDDDSDEDEEEADEEEEEEEAPVAKRGRKVTKVEAAPPRKPSSLDRALDRIDKALA
                     ARGRKTRSEPRGTGKLAKTKAGKAKKAAKRAATPIPNKKAAAAAAAAAAAAAADDDTE
                     AMSLDQAAKLLVPLVRKLKEATMQDLEEQTHMGRRKLRFHIGQLVKHGKLKRHGMGRG
                     TCYTVK"
     gene            3029..>3565
                     /locus_tag="VGB85_25925"
     CDS             3029..>3565
                     /locus_tag="VGB85_25925"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEY0137560.1"
                     /translation="MQQAKPRVPATAPAPARKPSRAAVRGDVRPNRRLGAPAPGRSAS
                     HTSGRPAPARATPRVRPLALLRALTRTQLGPRARRFIGAGMRLGVAFALAWGLLLGAQ
                     QGYQYVTTSARFEAKTVVFEQTPHLSADRLTELMGLAPGTNILAVDVDAVRRKIEADP
                     WVASATVSRQLPDTLHVAV"
BASE COUNT          592 a         1375 c         1164 g          434 t
ORIGIN      
        1 ccgtgcctcg aggccgccgg cttcgacccg gcggtgttcg gccgcttcaa gggcgtgatc
       61 gcctacaccg gcaagaaggg cggcgacctg cgcaaggcca gggcgttgcg ggccaagctc
      121 gagaaggaca tgaagaaggc cggcctcgac cccgccgacc ccgagctcgc caaggaccac
      181 cccaagctcg gcggcagcta tgcgacctgg aaggacctgc agacccgcat cgaggtgatc
      241 gaccacgcgc agcgacgcct gcgctgcgag aagatgttcg tctcggccga gggcgtcggc
      301 aagtacgaaa ccggcgtgtt cgacaccgcg accacccacg cgctcgcggc cttcgagaag
      361 aagcacgcga tcaaaggttg gggccacttc acgcaggaca acctcgccgc gctcgccctg
      421 tcgcagcagg agagcatgca ccgccgcctg ctgcgcgtcg tcgaggagcg cacggtgtcc
      481 ggcgccagca tcctcgagga cggatccgcc gcgcgctgga agccgaagtt tcgctggacc
      541 gacagcgccg gcaaggagca cgcgctgcgc gacgtcgtcg gcgaggccca ggccgccatg
      601 gtcgccgccc tcgacctcgc cacccccgag agcgcccgca agcagctcga ccgcctctcc
      661 gacctcggcg gcggcaactt cgacaacctg ctcgtcgccg tgcgcctgcc cccgccgccc
      721 gcctactacg ccgaggacat gaccttcgac gtcgtgatcg accgcggcga cgtctggtac
      781 gacttcccgt tcgacgccga gggcaacaag ctcgtccagc cgcgcgagcg ctacccgcac
      841 tttacgatct acgccgtggt gggcgagcag aagatcccgc tggtgcactg gcgcaccacg
      901 atcggctcgt ggcgcagcga gatgtacaac ggcgccgagc actacaagta caaggactcc
      961 gacgtcggcg accgcgtgtg gaaggagatc gtcgccgccc cggtctggat cccgccgacc
     1021 tcgacgccgc cgcgcgagct ggtccgccgc aagtggaagg acggcaaggt ccgcacggtc
     1081 gtcaactacg gcgagttcgg cccgagctac acctccgcgt acgggctcgt cgccgcctat
     1141 cacatcaagg agatcaagga cgccgagggc aactggaagg ccgacttcga caacggcatc
     1201 cgcacccacg gcagcgtcga ctacatgtcg atcctgcgca ggtactcgca cggctgccac
     1261 cgcctctaca acatgtcggc ggtgcgcctg ttctccttcg tgctcgccca ccacgagttc
     1321 acccgcgagg gtcaggtcaa gctcggcttt gcccgcgagt tcgcccacga gggcaagccg
     1381 ttcaagatca gcctcaagac tcgcggctat cgctacgatc tcgccacgcc gatcgccgtg
     1441 cacgtgctcg agggccgcgt ccgcggcaac cgcaagacgc cggtcgaggg ctacgtgccc
     1501 aagccgacgc ccgcgctcgc ccccggcgac gacctctcgc ccgactcgcc gcccgacgcg
     1561 accgccaccg acgtaacgcc ggtgacgccg atgccgatgc cgatgaaccc gcccgcgccc
     1621 gcacccgcgc ccctgtgacg caccaatcca tgtcgatgtc gccaggcccg atgtgcctgg
     1681 cgagcgacga cgcagcgcgg tcatgtcggg acggactcga cgacatgcag ctggatcgtg
     1741 tcgcccgcga cgcgcttgac cttgacgttt tgatcgtcgc tcatggcgcg ctccggtcgc
     1801 cggagtcggc cagcggcccg ggtttttcgt catgccgggg tcggtccggt gcccccctgc
     1861 gaaccgtgac cgtgcgaaaa gccgaggagt tacgcgccgc tgtcgctgtg caggcggccc
     1921 ctgggggtgt ggccccgacg gccagggaag caagcgaact cgcgcggacc ccttgagcac
     1981 gcgggggcga tgctggcaat atcccgcgcc gtgcaaccta acctcgcgtc cgggtttgat
     2041 gacagctttc gtcgccacct cgccgtcgaa cagcgacgcg ccgtgctcga cacgatcgcc
     2101 cgcgagctga cgcccgacac gaccctcggc gacatcgtcg acgccgcgca ggacctcggc
     2161 tgggccgaga acctcggcga gctgagcctc gccgagctcg cgctggcgct gctcggcgag
     2221 ccgccgaccg cgaccgcgcc cgcgcgcgcc cccgcggcga cccacgctcc ggacgaggac
     2281 gaggaggacg agctcgaggc cgaccaagag accgacgcgg acgaggacga cgactccgac
     2341 gaggacgagg aggaggccga cgaggaagaa gaagaggagg aggccccggt cgccaagcgc
     2401 ggccgcaagg tcaccaaggt cgaggccgcc ccgccgcgca agccgagcag cctcgaccgc
     2461 gcgctcgacc gcatcgacaa ggcgctggcc gcccgcggtc gcaagacccg cagcgagccg
     2521 cgcggcaccg gcaagctggc caagaccaag gccggcaagg ccaagaaggc cgcgaagcgg
     2581 gccgccacac cgatccccaa caagaaggcc gctgccgccg ctgccgccgc cgctgccgcc
     2641 gctgccgccg acgacgacac cgaggcgatg agcctcgacc aagccgccaa gctgctggtg
     2701 ccgctggtcc gcaagctcaa ggaggcgacc atgcaggacc tcgaggagca gacccacatg
     2761 ggtcgccgca agctgcgctt tcacatcggc cagctggtca agcacggcaa gctcaagcgg
     2821 cacggcatgg gtcgcggcac ctgctacacg gtgaagtgag gcactgcgcg catgcacagg
     2881 cccgcgctcg atcgacccac gcgccgccgg taacttgcca cggcgatccg ctgctagtaa
     2941 tggcgaggct cgtcgcttct ccgttatgag cctgccgctc ctccgccgtg accgccccgc
     3001 gttagccgcc ccgccgaaga atcggcgggt gcagcaggcc aaaccgcgcg tcccggccac
     3061 cgcgccggcg cccgctcgca agccgagccg cgcggccgtc cgcggcgatg tccgacccaa
     3121 ccgccgcctc ggcgcgccgg ccccgggccg ctcggccagc cacacctcag gccgccccgc
     3181 cccggcccgc gcgaccccgc gggtccgccc gctcgcgctg ctgcgcgcgc tgacccgcac
     3241 gcagctcggc ccgcgggcgc gtcgcttcat cggcgccggc atgcgcctcg gcgtcgcctt
     3301 cgcgctcgcg tggggcctcc tgctcggcgc ccagcagggc taccagtacg tgacgacctc
     3361 ggcccgcttc gaggccaaga ccgtcgtgtt cgagcagacc ccgcacctct cggccgaccg
     3421 cctgaccgag ctgatgggcc tcgcgccggg caccaacatc ctcgccgtcg acgtcgacgc
     3481 cgtgcgtcgc aagatcgaag ccgacccgtg ggtcgccagc gcgaccgtct cgcgccagct
     3541 gcccgacacc ctgcacgtcg ccgtc
//
LOCUS       DASWNJ010001562         2693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_311275, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001562 DASWNJ010000000
VERSION     DASWNJ010001562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311275"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VGB85_25930"
     CDS             <1..231
                     /locus_tag="VGB85_25930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137561.1"
                     /translation="AERGCVEGLFADAKVDYSAVLDCRAEAYARTATCLNSLTCTDTA
                     RGDCYKDLVEDIDACPDLPDDVEKDLNDCIYN"
     gene            247..999
                     /gene="deoC"
                     /locus_tag="VGB85_25935"
     CDS             247..999
                     /gene="deoC"
                     /locus_tag="VGB85_25935"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEY0137562.1"
                     /translation="MISDIGESSKATPAAGGASMLTRAELAASIDHTLLRATATRGEI
                     ETLCAEARPHRFASVCVQPVRVALARACLEGQVKVCTVIGFPLGADHASTKARAAELA
                     VADGADELDVVINLGLLRDGAHAEVVADLAGVVTAAQKHLLKVILETALLTPEEIDLA
                     CRLAVAAGADFVKTSTGFGPGGATVEAVARMRAAVGPKIGVKASGGIGDAATARAMLA
                     AGATRLGASASLKILAGWDTAVPTNMSGSTGY"
     gene            1002..1847
                     /locus_tag="VGB85_25940"
     CDS             1002..1847
                     /locus_tag="VGB85_25940"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEY0137563.1"
                     /translation="MQQGLESAERVRDADGRQYHIGLAPGEVARHIMLVGDPARATRV
                     AGMFDRIELERRNREYVSYTGEHQGLRVTVMGTGMGGGATEIAVIELCQVVDNPVMIR
                     CGSAGALQPDIALGDLVVTTGALRLENTTQYFVDNGYPAVADAQAVLALAGAAEAIGQ
                     RYHVGVTATAPGFYGAQGRRVPGFMPREPEIVARLAAQGVKNLEMETSTLLTLASFRG
                     FPAGAVCAIYATRTDNVFITPEQKDAAELACVRCGLGGLHTMAAMEAARGERPVWHPG
                     LPLKI"
     gene            complement(1854..>2693)
                     /locus_tag="VGB85_25945"
     CDS             complement(1854..>2693)
                     /locus_tag="VGB85_25945"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY0137564.1"
                     /translation="GQRETPCPVHRAAGALGLEVRTPARVRKDAAEHAAFAALGLDAA
                     VVAAYGLILPASVLAIPPLGCLNIHASLLPRWRGAAPIQAAILAGDAESGVTIMQMEE
                     GLDTGPVHRVLSTPIGPLETSGELFERLAPLGGQLLEQFLRELPDVPPPTPQDERGVV
                     LAPMLAKAEGLTRWTCPVGQVVDHVRGMDPWPGAFTGRGTLVLKLFRARPSEHARPDG
                     ATPGQVLAVDRAGMHVACAEGVVCIAEVQVVGGKRMPAQAYAAGKPFAAGESLIDPPT
                     PAE"
BASE COUNT          395 a         1006 c          944 g          348 t
ORIGIN      
        1 gccgagcgcg gctgcgtcga gggcctgttc gccgacgcca aggtcgacta cagcgccgtg
       61 ctcgactgcc gcgccgaagc ctatgcccgc accgcgacct gcctcaactc gctgacctgc
      121 accgacaccg cccgcggcga ctgctacaag gacctcgtgg aggatatcga cgcttgcccg
      181 gacctgcccg acgacgtcga gaaagacctc aacgactgca tctacaactg acgcgcgcgg
      241 gcccgcatga tcagtgacat cggtgagagc agcaaggcca cgccggccgc cggaggagcg
      301 agcatgctga cccgcgccga gctcgccgcg agcatcgacc acacgctcct gcgcgcgacc
      361 gcgacgcgcg gcgagatcga gacgctgtgc gccgaggccc gcccgcaccg cttcgccagc
      421 gtgtgcgtgc agccggtccg cgtcgcgctg gcgcgcgcct gtctcgaagg ccaggtcaag
      481 gtgtgcacgg tgatcggctt cccgctcggg gccgaccacg cgtcgaccaa ggcgagggcc
      541 gcggagctgg cggtggccga cggcgccgac gagctcgacg tcgtgatcaa cctcggcttg
      601 ctccgcgacg gcgcccacgc ggaggtcgtc gccgacctcg ccggggtcgt caccgccgca
      661 caaaaacacc ttctaaaagt gatcctcgag acggcgctct tgaccccgga ggagatcgac
      721 ctcgcgtgtc gcctcgcggt cgcggccggc gcggattttg taaagacctc gaccggcttc
      781 gggcccggcg gcgcgacggt cgaggccgtc gcccgcatgc gcgccgcggt cgggccgaag
      841 atcggcgtca aggcgtccgg gggcatcggc gacgccgcga ccgcgcgggc gatgctcgcc
      901 gcgggcgcga cccgcctcgg cgccagcgcc tcgctgaaga tcctcgccgg ctgggatacg
      961 gctgtcccca cgaacatgtc tggaagtaca ggttattaaa aatgcagcaa ggtcttgaat
     1021 ccgccgagcg cgtccgcgac gccgacggcc gccagtatca tatcgggctc gcgcccggcg
     1081 aggtcgcgcg tcacatcatg ctcgtcggcg acccggcgcg ggcgacccgc gtggccggca
     1141 tgttcgaccg catcgagctc gagcgccgca accgcgagta tgtcagctac accggcgagc
     1201 accagggcct gcgcgtcacg gtgatgggga ccggcatggg cggcggcgcg accgagatcg
     1261 cggtcattga gctctgccag gtggtcgaca acccggtgat gatccgctgc ggcagcgccg
     1321 gcgcgctcca gccggacatc gcgctcggcg acctcgtcgt cacgaccggc gcgctgcgcc
     1381 tcgagaacac gacgcagtac ttcgtcgata acggctaccc cgcggtcgcc gacgcccagg
     1441 cggtgctcgc cctcgccggc gcggccgagg cgatcgggca gcgctatcac gtcggcgtca
     1501 ccgcgaccgc ccccggcttc tacggcgcgc agggtcgccg cgtgcccggc ttcatgccgc
     1561 gcgagccgga gatcgtcgcg cgcctcgccg cgcagggcgt caagaacctc gagatggaga
     1621 cctcgacgct gctgaccctc gccagcttcc gcgggttccc ggccggcgcc gtatgtgcga
     1681 tctacgcgac gcgcaccgat aacgtgttca tcacgcccga gcaaaaggac gccgccgagc
     1741 tggcctgcgt gcgctgtggc ctcggcggcc tgcacacgat ggccgcgatg gaggccgccc
     1801 gcggcgagcg cccggtgtgg cacccgggcc tgccgctcaa gatctgagcg cgatcactct
     1861 gcgggcgtcg gcgggtcgat cagcgactcg cccgccgcga acggcttgcc cgccgcgtag
     1921 gcctgcgccg gcatgcgctt gccgccgacc acctgcacct ccgcgatgca cacgactccc
     1981 tcggcgcacg cgacatgcat gcccgcgcgg tcgaccgcca gcacctgacc gggggtcgca
     2041 ccgtcgggcc gcgcgtgctc cgagggccgg gcgcggaaca gcttgagcac cagggtgccg
     2101 cgacccgtga acgcgccggg ccacgggtcc atgccgcgca cgtggtcgac gacctgtccc
     2161 acgggacatg tccatcgggt caggccctcg gccttggcga gcatcggcgc gagcaccacg
     2221 ccgcgctcgt cctgcggggt cggcgggggc acgtccggca actcgcgcag gaactgctcg
     2281 agcagctgcc ccccgagcgg ggcgaggcgc tcgaacagct cgccgctggt ctcgagcggg
     2341 ccgatcgggg tcgacagcac gcggtggacc gggccggtat cgaggccctc ctccatctgc
     2401 atgatggtca cgccgctttc ggcatccccg gcgaggatcg ccgcctggat cggcgccgcg
     2461 ccgcgccagc gcggcagcag cgaggcatgt atgttcaggc aacccagcgg cggtatcgcc
     2521 agcactgacg ccggcaggat cagcccatag gcggcgacga cggcggcatc gaggccgagc
     2581 gcggcgaagg cggcatgctc ggccgcatcc ttccgcaccc gtgccggggt ccgcacctcc
     2641 agccccagcg cgcctgcggc ccggtggacc gggcaggggg tttcccgctg gcc
//
LOCUS       DASWNJ010001563         7464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_663978, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001563 DASWNJ010000000
VERSION     DASWNJ010001563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7464
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663978"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VGB85_25950"
     CDS             complement(<1..705)
                     /locus_tag="VGB85_25950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137565.1"
                     /translation="MSSSSLSRALAVACLLALAPSPGAAAPVKAKSAKVKASKAKPEE
                     PAATPAATTQPAATTTQSATPSAAAGSVADPFAQAGSATVTGQPAGARESEIYGDRLD
                     GIEGEVAALKDKIFRSKARLAVLRDTVMSGVMAGGRVVVAHRNLMSSSFRLVRVAVIL
                     DGAQIFARSDDTGALDQEDELPLFDGNLPPGPHEMTIELSYQGQGYGAFSYLKGYSFK
                     SASSHSFNVGDSGQFKL"
     gene            complement(843..3671)
                     /locus_tag="VGB85_25955"
     CDS             complement(843..3671)
                     /locus_tag="VGB85_25955"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0137566.1"
                     /translation="MSRPSRLVHNASRLVDLRLPNGARILVLPLPGGPGERSRGSDER
                     GRGEYGDGDRDRRDDGHRGNREHDRGERGPGDRYRLTRSGGNGAAAVQLWVGAGTSAE
                     RPDEHGCAHLLEHMLFKPLPEEHVPRNMLRALGVPATGEIDLASALEALGGDSNAFTS
                     HDETVIHATVPARAAATAVAIIGAAVLHPRLAAATLAQEREVVVEEIKQYDDEPGQRV
                     FQALLRSIHGRHSYSRPVLGLTREVRGHTPARLRAYHRRAYAGQRLTLVVVGDVEVAK
                     VVATARRALEPLPRARPLPDEADPRRHSPGPRVHLRRGDVQEVHLMIGWAAPAAGDED
                     APALDVASVVLGHGEASRLVRETRRRDQVVSEVSCSCESSRRRGSFIVHANTTAPQAR
                     DAVAALFAQVARLREHVIDADELARARAILESDPVYRQETVQGRAHAIGYYATAFGDL
                     GRERAYYAELAQLTPESVRDACERYLAVTQASVSAELPSEDTTPAALERLTRAIAAEA
                     PARPKARARRPAIARDRFGVASVDLANGLSVRAIVDPSVRMAAGWLVWPGGQSTEPSA
                     LAGAAATIAALLTRGNDRHDGDEISHAVDGRAASLDGFAGRNSLGLRWECLSRDVPAI
                     LELALECGRSPSFPGDELAEERRVALQELAAEADDPAQLAIRAMSAAFYGDHPFHRPI
                     RGTPSGLRAQSAPRLRRLWADSYAPGDAVLGIVGDFDLEDTLARMELLLGTGRKGQAR
                     SPRARAPTPARLPGGPRERVIYKEREQAHLALGFPGLLLGDPQGPALDILNAVLGGQS
                     GRLFAALRERQGLVYEISVSSVEGRDAGHVMVHASTGQDKLPRARAAIAAELERVVAE
                     AITREELARAQAWLVGQHESGQQRRSRVASQLAFAAAHGLDYGRHFLYPERVAAVTAP
                     QVLALARRIFDPRRQVTGLVRARQGRAS"
     gene            3909..>7464
                     /locus_tag="VGB85_25960"
     CDS             3909..>7464
                     /locus_tag="VGB85_25960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137567.1"
                     /translation="MDLAAAAPHTSSLKDLPCPLPPPAVGEPVELGAYRKSPGSDDAF
                     LRARRSLREAENWAALASLLVLHAAAIRDPAKVGELSFQAYELWNDRVKDRTQAAHAL
                     VRALQAQPENVRPYEHLRKLYEGLGAHTELATLLRWRIEHLRRHDRGAVPAALIELGQ
                     LYENQFYDIHEATVLYRKALEQSPNDRQASEQLIRLYLAAGAWPRAMELINAELARTD
                     PAREKARFAELHLRLARIESQQLENIPAAAVHLQAALKAGPDNIAALRAFGVLYLGSG
                     KASNEGLAKAADVFFRAAKLARAQSDDRQALGLLRRTLALKPDHFEAGNALAELLAAK
                     ERWMELDELYGAWLGYISEADSYSLWLQRGELLETHLSRREEARACFAMASRFEAPGG
                     DAWLRLEALLRELSDHHGYVGLIERWVEQAPDSTPTARLLSAARVAREELGDEERAAV
                     FFYKVLEREPFNAEAFEGYKEHWRRKNNWTHLAELLLYQIDQAQQIQGAQSPLARSDF
                     AENFAELADVYERRLGDLGGALNAWNRMAAAYPQDWRPREQIGRIDKRARLLDNMVVS
                     QENELARTHEPARRFEVLRRLTQAQRERMTDPQRTITLYHEMLGLAPGDAATIKALGE
                     MYERTGAFDSVIDLLRAQHDATRSVSQRVVLLRRMAEIWHNELRQPREALWACEQILG
                     YSQGDSDALHRMQSLCAELAEAAGEFDALARELQLVGEPAQRARVLRRMVDVAERKLQ
                     DPQRTAQACQQLLSLDPHNMEVLDKMIAVYDAIGRHEELANLLGKAAASAKTPPIRQL
                     DYLMRLGHLAETTLADHDLACSAFERVLRIHRDHRGAVEALTRLYRSLGSWHGLAAAL
                     GNLQDMVDSDEEAIAIGWERAEVLADKLDNPAAAVRVLEQLASGVAIGNRDIAARLLE
                     LYERASQFDKLIRHAEVMLLSITDLDQRRELYEMIARSWLVHFQAQDKALASFQRFLA
                     ENPDDADGLRIMGELQTLTGDHAGTLATLERRLELSRDPAEQVVTLEQMAEVCETGLG
                     HAKRALQLLGRALSIAPGSRDLKHKIEAFAEAHRMWKDLLVVYGERFTEMNARGESRG
                     QIEICLSASTAAEQRIGDVELAFAWAKKGYFVALRAGMDAAAVLDRLETLAQQHGLWA
                     QMLEVHEQELALQESSKSPNYGDYATIALLLSAAEVARE"
BASE COUNT         1035 a         2764 c         2696 g          969 t
ORIGIN      
        1 gagcttgaac tggccgctgt cgccgacgtt gaagctgtgc gacgaggccg acttgaagct
       61 gtagcccttg aggtagctga aggcgccgta gccctggccc tggtacgaca gctcgatcgt
      121 catctcgtgc gggccgggcg ggaggttgcc gtcgaacagc ggcagctcgt cctcctggtc
      181 gagcgcgccg gtgtcgtcgc tgcgcgcgaa gatctgggcg ccgtcgagga tcaccgcgac
      241 gcggaccagg cgaaagctcg agctcatcag gttgcggtgg gcgacgacga cgcggccgcc
      301 cgccatcacg ccgctcatca ccgtgtcgcg cagcaccgcg aggcgggcct tgctgcggaa
      361 gatcttgtcc ttgagcgccg cgacctcgcc ctcgatgccg tcgaggcggt cgccgtagat
      421 ctcgctctcg cgggcgcccg cgggctggcc cgtcaccgtg gccgagcccg cctgcgcgaa
      481 ggggtcggcg acggagcccg cagcggcgga cggggtcgcg gactgcgtcg tcgtcgcggc
      541 cggctgcgtc gtcgcggccg gggtcgcggc cggctcctcg ggcttggcct tcgaggcctt
      601 cacctttgcg gacttcgcct tcaccggcgc cgccgcgccc ggcgacgggg ccagggcgag
      661 caggcaggcg acggcgagcg cccgggacag cgaggaggat gacacgcggg cgagcctagc
      721 aaggcgcgga ccggcaaggc cagtccgttg catttaccgg gcccggcgcc gcccggaaac
      781 tggagcgcat cggccgcgat ttcgcgctta ccttcaggaa cgcgcatgcc tccaggaatt
      841 cgtcacgacg cccggccctg gcgcgcgcgc acgaggccgg tgacctggcg ccgggggtcg
      901 aagatcctgc gcgcgagggc gaggacctgc ggggcggtga cggcggcgac gcgctcggga
      961 tagaggaagt ggcgcccgta gtcgaggccg tgcgcggccg cgaaggccag ctgcgaggcg
     1021 acgcggctgc ggcgttgctg gccgctctcg tgttgcccga cgagccaggc ctgggcccgc
     1081 gcgagctcct cgcgggtgat cgcctcggcg acgacgcgct cgagctcggc ggcgatcgcc
     1141 gcccgggcgc gcgggagctt gtcctggccg gtcgacgcgt ggaccatgac gtggccggcg
     1201 tcgcggccct cgacgctgct gaccgagatc tcgtagacga ggccctggcg ctcgcgcagc
     1261 gccgcgaaca agcgcccgct ctggccgccg agcaccgcgt tgaggatgtc gagcgccggg
     1321 ccctgcgggt cgccgagcag cagccccggg aagccgagcg cgaggtgggc ctgctcgcgc
     1381 tccttgtaga tcacgcgctc gcgggggccg cccggcagcc tcgccggcgt cggcgcgcgc
     1441 gcgcgtggag atctggcctg gcccttgcga cctgtcccga ggagcagctc catgcgggcg
     1501 agcgtgtcct cgaggtcgaa gtcgccgacg atgccgagca ccgcgtcgcc gggcgcgtag
     1561 ctgtcggccc acagccggcg cagccgcggg gcgctctgcg ctcgcagacc ggacggggtg
     1621 ccgcggatcg gccggtggaa ggggtggtca ccgtagaagg ccgccgacat cgcgcggatc
     1681 gccagctgcg cggggtcgtc ggcctcggcg gcgagctctt gcaaggcgac gcggcgctcc
     1741 tcggcgagct cgtcgccggg gaagctcggc gagcggccgc actcgagcgc gagctcgagg
     1801 atcgcaggca cgtcgcggct gaggcactcc cagcgcaggc cgaggctgtt gcgcccggcg
     1861 aagccgtcga gcgaggcggc gcggccgtcg accgcgtggc tgatctcgtc gccgtcgtgg
     1921 cggtcgttgc cgcgggtcag cagcgcggcg atcgtcgccg ccgcgcctgc cagcgcggag
     1981 ggctcggtgc tctggccgcc gggccacacc agccagcccg ccgccatgcg cacgctcggg
     2041 tcgacgatcg cacgcacgct caggccattg gcgaggtcga cgctggcgac gccgaagcgg
     2101 tcgcgggcga tcgcggggcg gcgcgcgcgg gccttcgggc gcgccggggc ctcggcggcg
     2161 atcgcccgcg tcaggcgctc gagcgcggcg ggggtggtgt cctcggaggg cagctcggca
     2221 ctgacgctcg cctgcgttac cgcgaggtag cgctcgcagg cgtcgcgcac cgactcgggg
     2281 gtcagctggg cgagctcggc gtagtaggcc cgctcgcggc cgaggtcgcc gaacgcggtc
     2341 gcgtagtagc cgatcgcgtg ggcccgaccc tgcacggtct cctggcgata cacggggtcg
     2401 ctctcgagga tcgcgcgcgc gcgtgccagc tcgtcggcgt cgatcacgtg ctcgcgcaga
     2461 cgggcgacct gggcgaacag cgccgcgacc gcgtcgcggg cctgcggggc ggtggtgttg
     2521 gcgtggacga tgaagctgcc gcgccgccgc gacgactcgc agctgcagct gacctccgac
     2581 acgacctggt cccggcgtcg ggtctcgcgg accaggcgcg aggcctcgcc gtgtccgagc
     2641 accaccgacg cgacgtcgag ggccggtgcg tcctcgtcgc cggcggccgg cgccgcccag
     2701 ccgatcatca ggtggacctc ctggacatcg ccgcggcgca ggtgcacgcg cggaccggga
     2761 ctatggcgcc gcgggtcggc ctcgtcgggc aagggtcgcg cgcgcggcag gggctcgagc
     2821 gcgcggcggg ccgtggcgac gaccttcgcc acctcgacgt cgccgacgac cacgagggtc
     2881 aggcgctggc cggcgtaggc gcggcgatgg taggcgcgca ggcgggcggg cgtgtggccg
     2941 cggacctcgc gggtcagccc gagcaccggc cggctgtagc tgtggcgccc gtggatgctg
     3001 cgcagcagcg cctggaacac gcgctgcccg ggctcgtcgt cgtattgctt gatctcctcg
     3061 acgacgacct cgcgctcctg cgccagcgtg gcggcggcga gccgcgggtg cagcaccgcc
     3121 gcgccgatga tcgccaccgc cgtcgccgcg gcgcgggccg ggaccgtcgc gtggatcacc
     3181 gtctcgtcgt ggctggtgaa cgcgttgctg tcgccgccca gcgcctcgag cgcgctggcg
     3241 aggtcgatct ccccggtcgc cggcaccccg agcgcgcgca acatgttcct cgggacatgt
     3301 tcctcgggca agggtttaaa gagcatgtgc tcgagcaggt gggcgcagcc gtgctcgtcc
     3361 gggcgctccg ccgaggtgcc ggcgccgacc cacagctgca ctgcggccgc cccgttgccg
     3421 ccggaccggg tcagccgata gcggtcgccc ggcccccgct cgccgcggtc gtgctcgcgg
     3481 ttgccacgat gaccgtcgtc gcggcgatcc ctatcgccgt cgccgtactc gccgcggccg
     3541 cgctcgtccg acccgcggct gcgctcaccg ggcccgcctg gcaggggcag cacgaggatc
     3601 cgggcgccgt tgggcaggcg gaggtcgacg agcctggagg cgttgtggac gagtcgcgac
     3661 ggacgagaca aggccgcgac ctatagcgca tcccttgccg cccgtcgaac cgccatcaac
     3721 ctgcgccaga gccaacgatc gggcatgtac gcagggtttt gcggggttcg tgcgacaacg
     3781 ccgaggggac cccccgcccg cgccgcagat ccaggctttg acgcccgggg agccccgccg
     3841 gtgcccttcc cggccgagcg gacggcgtgg tacggtgtgc gctcgcgccc ttctggaccc
     3901 gaaaactcat ggacctcgcc gctgcggccc cgcacacctc ctcgctgaaa gacctgccct
     3961 gtccgctgcc gccgccggcc gtcggtgagc cggtggagct cggcgcgtat cgcaagtcgc
     4021 cgggcagcga cgatgcattc ctgcgcgcgc gcaggagcct tcgtgaggcc gagaactggg
     4081 ccgcgctggc gagcctgctg gtgttgcacg ccgcggcgat ccgcgacccg gccaaggtcg
     4141 gcgagctgtc gttccaggcc tatgagctgt ggaacgaccg cgtcaaggac cggacccagg
     4201 ccgcgcacgc cctcgtgcgc gccctgcagg cccagccgga gaacgtgcgg ccctacgagc
     4261 acctgcgcaa gctctacgag ggcctcggcg cgcacaccga gctggcgacg ctgctgcgct
     4321 ggcgcatcga gcacctgcgg cggcacgacc gcggcgccgt gccggccgcg ctgatcgagc
     4381 tcggccagct gtacgagaac cagttctacg acatccacga ggcgaccgtc ctctaccgca
     4441 aggcgctcga gcagagcccc aacgaccgcc aggccagcga gcagctgatc cgcctgtacc
     4501 tcgccgccgg cgcgtggccc agggcgatgg agctgatcaa cgccgagctc gcgcgcaccg
     4561 accccgcccg cgaaaaggcc cgcttcgccg agctgcacct gcggctcgct cgcatcgagt
     4621 cccagcagct cgagaacatc cccgccgccg ccgtgcacct gcaggccgcg ctcaaggccg
     4681 ggcccgacaa catcgcggcg ctgcgcgcct tcggcgtgct ctacctcggc tccggcaagg
     4741 ccagcaacga gggcctcgcc aaggccgccg acgtgttctt ccgcgccgcc aagctggccc
     4801 gcgcgcagag cgacgaccgc caggccctgg gcctgctgcg tcgcacgctc gcgctcaagc
     4861 ccgaccactt cgaggccggc aacgcgctcg ctgaattgct ggcggccaag gagcgctgga
     4921 tggagctcga cgagctctac ggcgcctggc tcggctacat cagcgaggcc gacagctact
     4981 cgctgtggtt gcagcgcggc gagctgctgg agacgcacct gtcgcgccgc gaggaggcgc
     5041 gcgcctgctt cgccatggcc tcgcgcttcg aggcgcccgg cggcgacgcg tggctgcgcc
     5101 tcgaggccct gctgcgcgag ctgagcgacc accatggtta tgtcggcttg atcgaacggt
     5161 gggtcgagca ggcgccggac agcacgccca cggcacgact gctgagcgcc gcccgcgtcg
     5221 cccgcgagga gctcggcgac gaggagcgcg ccgctgtgtt cttctacaag gtgctcgagc
     5281 gcgagccctt caacgccgag gccttcgagg gctacaagga gcactggcgc cgcaagaaca
     5341 actggaccca cctcgccgag ctgctgctct accagatcga tcaggcccag cagatccagg
     5401 gcgcccagag cccgctcgcg cgcagtgact tcgccgagaa cttcgccgag ctcgccgatg
     5461 tctacgagcg ccgcctcggt gacctcggcg gggcgctcaa cgcctggaac cgcatggccg
     5521 ccgcctaccc gcaggactgg cgcccgcgcg agcagatcgg ccgcatcgac aagcgcgcgc
     5581 gcctgctcga caacatggtc gtctcgcagg aaaacgagct cgcccgcacc cacgagcccg
     5641 cgcgaaggtt cgaggtcctg cggcggctga cgcaggccca gcgcgagcgg atgaccgacc
     5701 cccagcgcac gatcaccctc tatcacgaga tgctgggcct cgcgcccggc gacgccgcga
     5761 cgatcaaggc gctcggcgag atgtacgagc gcaccggcgc cttcgactcc gtgatcgacc
     5821 tgctgcgggc ccagcacgac gcgacccgat cggtgtcgca gcgcgtggtg ctcctgcgcc
     5881 gcatggccga gatctggcac aacgagctcc ggcagccgcg cgaggcgctg tgggcctgcg
     5941 agcagatcct cggctactcg cagggcgaca gcgacgccct gcaccgcatg cagagcctgt
     6001 gcgccgaatt ggccgaggcc gcgggcgagt tcgacgcgct cgcgcgcgag ctgcaactcg
     6061 tcggcgagcc ggcccagcgc gccagggtgc tgcggcgcat ggtcgacgtc gccgagcgca
     6121 agctgcagga cccgcagcgg accgcgcagg cctgccagca gctgctgtcg ctcgacccgc
     6181 acaacatgga ggtcctcgac aagatgatcg ccgtgtacga cgcgatcggt cgccacgagg
     6241 agctcgccaa cctgctcggc aaggcggccg cctcggccaa gaccccgccg atccgccagc
     6301 tcgactacct catgcgcctc ggccacctcg ccgagacgac cctcgccgac cacgacctcg
     6361 cctgctccgc cttcgagcgc gtgctgagga tccaccgcga ccaccgcggc gccgtcgagg
     6421 cgctgacccg cctgtatcga tcgctcggct cgtggcacgg cctcgccgcg gcgctcggca
     6481 acttgcagga catggtcgac tccgacgagg aggcgatcgc gatcggctgg gagcgcgccg
     6541 aggtgctcgc cgacaagctc gacaaccccg ccgccgccgt ccgcgtgctc gagcagctcg
     6601 cgtcgggcgt agcgatcggc aaccgcgaca tcgcggcccg cctgctcgag ctgtacgagc
     6661 gcgccagcca gttcgacaag ctgatccgcc acgcggaggt catgctgctg tcgatcaccg
     6721 acctcgacca gcgccgcgag ctctacgaga tgatcgcgcg aagctggctc gtccacttcc
     6781 aggcccagga caaggcgctc gccagcttcc agcgcttcct ggccgagaac ccggacgacg
     6841 ccgacggcct gcgcatcatg ggcgagctgc agaccctcac cggcgaccac gccggcacgc
     6901 tcgccacgct cgagcgccgc ctcgagctgt cgcgcgaccc cgccgagcag gtcgtcaccc
     6961 tcgagcagat ggccgaggtg tgcgagaccg gcctcggcca cgccaagcgg gccctgcagc
     7021 tcctcggccg cgcgctctcg atcgcccccg gcagccgcga cctcaagcac aagatcgagg
     7081 ccttcgccga ggcccaccgc atgtggaagg acctgctcgt cgtctacggc gagcgcttca
     7141 ccgagatgaa cgcccgcggc gagagccgcg gccagatcga gatctgcctc agcgcttcca
     7201 cggccgccga gcagcgcatc ggcgacgtcg agctcgcgtt cgcgtgggcc aagaagggct
     7261 acttcgtggc cctgcgcgcc ggcatggacg ccgctgccgt gctcgaccgc ctcgagaccc
     7321 tcgcccagca acacggcctc tgggcccaga tgctcgaggt gcacgagcag gagctcgcgc
     7381 tgcaagagtc gagcaagtcg cccaactacg gcgactatgc caccatcgca ttgctcctat
     7441 ccgccgcgga ggtcgcccgc gagc
//
LOCUS       DASWNJ010001564         1810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_521160, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001564 DASWNJ010000000
VERSION     DASWNJ010001564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521160"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1692
                     /locus_tag="VGB85_25965"
     CDS             <1..1692
                     /locus_tag="VGB85_25965"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY0137568.1"
                     /translation="KEIPLAEGTCVTIMANTRLEWVLCDFAMLSLGLRTVPIYVSLLP
                     PEIGYLHADTAAELLVVENREMLEKVRATREGFNFYDKQYEGSRIKLRRAIVIDPTGL
                     APADDWESLEQVESRGRESLTATQAERDRRMAAIKRTDVATYTYTSGTTGEPKGAIQT
                     HDNMLSILENVADAQLFTDRAKIGGAFLFLPLAHSFGRMVEFGGAFYMTKVVFSSIAT
                     VADDLRMARPGFFPAAPRVFERVYGKIMTGIADAPAVRQKLFHWAIGVGKQTIPYRQK
                     NKPLPLGLRMQFAVADKLVLSKIRGKLGMDRMHVLMSGSAPLPREVADFFAGAGLVVL
                     EAYGLTETCPGVSVNRFDDWRIGTVGPAIKGVQIKLAADGEILVKGPNVVSGYLNRPE
                     ATAEAWDEDGWFHTGDIGELDPDGHLRITDRKKDLIKTSGGKYVAPQKIEALLKTKPI
                     LSEAVVIGDNRKYCVALVTVDKEKLAAWAERTGNSPDLQGEAVQKEVQSYLDEANKGL
                     ASFETVKYFKVVDEEFSVDNGLLTASFKVKRKAVSAKYKDLIDSMYTGKEKDKAE"
BASE COUNT          324 a          596 c          620 g          270 t
ORIGIN      
        1 aaggagatcc cgctggcgga gggcacgtgc gtcacgatca tggccaatac gcggctcgag
       61 tgggtgctct gcgactttgc gatgctgtcg ctcggtctgc gcacggtgcc gatctacgtc
      121 agcctgctcc cgccggagat cggctacctg cacgccgaca ccgccgcgga gctgctggtg
      181 gtcgagaacc gcgagatgct cgagaaggtc cgcgcgacgc gcgagggctt caacttctat
      241 gacaagcagt acgagggctc gcggatcaag ctgcgtcgcg cgatcgtcat cgacccgacc
      301 ggcctggcgc cggccgacga ctgggagagc ctcgagcagg tcgagtcgcg cggccgcgag
      361 tccctgaccg cgacgcaggc cgagcgtgac cgccggatgg cggcgatcaa gcgcacggac
      421 gtcgccacct acacgtacac ctccgggacc acgggcgagc ccaagggcgc gatccagacc
      481 cacgacaaca tgctgtcgat cctcgagaac gtcgccgacg cgcagctgtt caccgaccgc
      541 gccaagatcg gcggggcctt cctgttcttg ccgctggcgc actcgttcgg gcgcatggtc
      601 gagttcggcg gggcgtttta catgaccaag gtcgtgttca gctcgatcgc cacggtcgcc
      661 gacgacctcc gcatggcgcg tccgggcttc ttcccggcgg cgccgcgggt gttcgagcgc
      721 gtctacggca agatcatgac cgggatcgcc gacgcgccgg cggtccggca aaagctgttc
      781 cactgggcga tcggggtcgg caagcagacg atcccctacc gccagaagaa caagcccctg
      841 cccctcgggc tgcggatgca gttcgccgtc gccgacaagc tggtgctgtc gaagatccgc
      901 ggcaagctcg gcatggaccg catgcacgtg ctgatgtcgg gctcggcccc gctgccgcgc
      961 gaggtcgccg acttcttcgc cggcgccggg ctcgtggtgc tcgaggccta cggtctgacg
     1021 gagacctgcc cgggcgtgtc ggtcaaccgc ttcgacgact ggcggatcgg cacggtcggc
     1081 ccggcgatca agggcgtgca gatcaagctc gccgcggacg gcgagatcct cgtcaagggg
     1141 ccgaacgtgg tctccggcta cctcaaccgc ccggaggcga ccgccgaggc gtgggacgag
     1201 gacggctggt tccacaccgg cgacatcggc gagctcgacc ccgacggcca cctgcggatc
     1261 accgaccgca agaaggacct gatcaagacc agcggcggca agtacgtcgc gccgcagaag
     1321 atcgaggcgc tgctcaagac caagccgatc ctgtcggagg cggtcgtcat cggcgacaat
     1381 cgcaagtact gcgtcgcgct ggtgacggtc gacaaggaga agctcgcggc gtgggccgag
     1441 cgcaccggca actcgcccga cctgcagggc gaggcggtcc agaaggaggt ccagagctac
     1501 ctcgacgagg ccaacaaggg cctcgccagc ttcgagacgg tcaagtactt caaggtcgtc
     1561 gacgaggagt tctcggtcga caatggcctg ctgaccgcgt cgttcaaggt caagcgcaag
     1621 gcggtgtccg ccaagtacaa ggacctcatc gactcgatgt acacgggcaa ggagaaggac
     1681 aaggccgagt aggccgtcct cgccgtgtga atcaagggga catgcccgta cgggcatgtc
     1741 cctttgttca tgtccagatt cgtcagcgca gcgggccggt gacggagccg agcttgacga
     1801 acgcgtcgtc
//
LOCUS       DASWNJ010001565         2111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_339591, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001565 DASWNJ010000000
VERSION     DASWNJ010001565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339591"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..683)
                     /locus_tag="VGB85_25970"
     CDS             complement(45..683)
                     /locus_tag="VGB85_25970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0137569.1"
                     /translation="MADDDLALLEAWRAGDRAAGEALLARHFDAVCRFFHNKVGDDAP
                     DLIQQTFLALCEGRERFRGDGAFRGYMLGTARNVLLAHFRRKRKQAERFDPLEHSVID
                     QQASFTSQLAQRREEQILLQALRELPLDLQIVFELYHLEGLTAGELAAMHGISEPGMY
                     GRLRKARLMTIAACERLAETPALRRSTVERLDDWAAELRARLGTAPGASDMS"
     gene            complement(702..1934)
                     /locus_tag="VGB85_25975"
     CDS             complement(702..1934)
                     /locus_tag="VGB85_25975"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0137570.1"
                     /translation="MNIFATIRIAVLALLRNKMRSFLTVLGIIIGVAAVIAMVAIGEG
                     AKQEVQQAFDKMGTNMLVVRSGSSQMGGVRGGAGSQPTLTWTDVDAIRNEAGGVAMGA
                     PSMQQRNTPLLGDGQNWTTTAEGTTPEYFPIRAWTFQYGGAFTESDVTAKTKVAVLGG
                     TVADNLFGPGSNPDGSDVRIANMPFEVVGVLSRKGQSAFGQDTDDVVIIPVTTFATKI
                     QGGLKKYLQGTIYVSAASAADTKRAQLQIEEVLRRRHGLRETQDSDFTVNNLAEAASA
                     RAEGTETMTRLLAGIALVSLLVGGIGIMNIMLVSVTERTREIGLRMAIGAKPGDIRTQ
                     FVIEAMTLSMIGGAVGVAIGVGAAEYLRIYVGWSTVVQPQIVAVSLGFSAVVGVGFGL
                     YPAHKASGLDPIQALRFE"
     gene            complement(1931..>2111)
                     /locus_tag="VGB85_25980"
     CDS             complement(1931..>2111)
                     /locus_tag="VGB85_25980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137571.1"
                     /translation="LFQELGRSGITIVLVTHEPDIASFTNRVILMRDGKMQFDRQQTP
                     VDARAAADAYAEVPE"
BASE COUNT          318 a          756 c          709 g          328 t
ORIGIN      
        1 cggaccgaca atctcctgac ccactgagcg aggactgctg accttcagga catgtccgaa
       61 gcgcccggcg ccgttccgag gcgcgcgcgc aactccgcgg cccagtcgtc gagccgctcg
      121 accgtcgagc ggcgcagcgc cggggtctcc gcgagccgct cgcacgcggc gatcgtcatc
      181 agcctggcct tgcgcagccg gccgtacatg cccggctccg agatcccgtg catcgccgcc
      241 agctcgcccg cggtcaagcc ctcgaggtga tacagctcga acacgatctg cagatccagc
      301 ggcagctcgc gcagcgcctg cagcaggatc tgctcctcac gccgctgcgc gagctggctg
      361 gtgaaggacg cctgttgatc gatcacgctg tgctcgagcg ggtcgaagcg ctcggcctgc
      421 ttgcgcttgc gacgaaagtg ggcgagcagc acgttgcggg ccgtgccgag catgtagccg
      481 cgaaacgccc cgtcgccgcg aaaccgctcg cggccctcgc acagcgccaa gaacgtctgc
      541 tggatcaggt cgggcgcgtc gtcgccgacc ttgttatgaa agaaccgaca caccgcgtcg
      601 aagtgacggg cgagcagcgc ctcgccggcc gcgcggtcgc ccgcgcgcca ggcctcgagc
      661 agcgcgaggt cgtcgtccgc caccccgcca cggtaccacg ctcactcgaa gcgcagcgcc
      721 tggatcgggt cgaggcccga ggccttgtgc gccgggtaca ggccgaagcc gacgccgacg
      781 accgcgctga agccgagcga cacggcgacg atctgcggct gcacgaccgt cgaccagccg
      841 acgtagatcc gcaggtactc ggccgcgccg acgccgatcg ccacgccgac cgcgccgccg
      901 atcatcgaca gggtcatcgc ctcgatcacg aactgcgtgc ggatgtcacc cggcttggcg
      961 ccgatcgcca tgcgcaggcc gatctcgcgg gtccgctcgg tgacgctgac gagcatgatg
     1021 ttcatgatcc cgatgccgcc gaccagcagg ctgacgagcg cgatcccggc gagcaggcgc
     1081 gtcatcgtct ccgtgccctc ggcgcgcgcg ctcgcggcct cggcgaggtt gttgaccgtg
     1141 aagtcgctgt cctgcgtctc gcgcaggccg tgccggcgac gcagcacctc ttcgatctgc
     1201 agctgcgcgc gcttggtgtc cgccgccgac gcggccgaca cgtagatcgt gccctgcagg
     1261 tacttcttga ggccgccctg gatcttggtg gcgaacgtcg tcaccgggat gatcacgacg
     1321 tcgtcggtgt cctggccgaa cgccgactgg cccttgcggc tgagcacgcc gacgacctcg
     1381 aagggcatgt tggcgatccg cacgtcgctg ccgtccgggt tggagccggg cccgaacagg
     1441 ttgtccgcga ccgtgccgcc gagcaccgcg accttggtct tggcggtgac gtcggactcg
     1501 gtaaaagccc ccccgtactg gaaggtccac gcgcggatcg gaaaatactc gggcgtcgtg
     1561 ccctcggccg tggtcgtcca gttctggccg tcgccgagca gcggtgtgtt gcgctgctgc
     1621 atgctcggcg cgcccatcgc gaccccgccg gcctcgttgc ggatcgcgtc gacgtcggtc
     1681 caggtcagcg tcggctgcga gccggcgccg ccgcgcacgc cgcccatctg cgacgagccc
     1741 gagcgcacca ccagcatgtt ggtgcccatt ttatcaaagg cctgctggac ctcctgcttg
     1801 gcgccctcgc cgatcgccac catcgcgatc acggcggcga cgccgatgat gatgccgagc
     1861 accgtcagga acgatcgcat cttgttgcgc agcagcgcga gcacggcgat ccggatcgtt
     1921 gcaaaaatgt tcactcgggg acctccgcgt aggcgtcggc ggccgcgcgg gcgtcgaccg
     1981 gggtttgctg gcgatcgaac tgcatcttgc cgtcgcgcat caggatcacg cgattggtga
     2041 acgaggcgat gtcgggctcg tgggtgacga gcacgatcgt gatcccgctg cgcccgagct
     2101 cctggaacag c
//
LOCUS       DASWNJ010001566         2927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_559723, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001566 DASWNJ010000000
VERSION     DASWNJ010001566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559723"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VGB85_25985"
     CDS             <1..786
                     /locus_tag="VGB85_25985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137572.1"
                     /translation="GLPADCASEVCTAGMCAPAGPLCAQQPADPVTGQRCPLFMACTQ
                     SSECGDFKGCQQWFCNNAKRCELNALVGCWTDKGGACNADVVFTQQTAPPVDKRFVPP
                     DNVDFRELASLAFTIKNNSASDLYLDKLPLVLDVIGGGSKFDVASVKIFDNSGGTEHG
                     GGDLLVCLTANPFSFPANGVMGPCGGSAFAKIPKGQSNQFIVNVAFAKEKTFIAGRGY
                     RLRLDSTTGIELKNGFNGPVYQGSMCGVPPGGYAGAWVTAQNP"
     gene            952..1929
                     /locus_tag="VGB85_25990"
     CDS             952..1929
                     /locus_tag="VGB85_25990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose phosphate epimerase"
                     /protein_id="HEY0137573.1"
                     /translation="MRSTLLKLCLLPTLVALAGCGKDSENMTMATAPGAETTGGDGTT
                     SGGQSETGAVVTGEPTTTGAQQTTTGAETSAANTGDSSGSTGSSFLTPPDGGSVNDME
                     CSVWDQDCPVDQKCMPWANNGSTAWNATKCVPVDPAKGQPGDTCTVEGSAVSGLDTCD
                     LGVLCWDVKPATMMGTCVAQCKGPESDPTCDANSSCFISNDGVLTLCLPKCDPLTQDC
                     PNENLCIPNPQNQEEFTCVLDASGDGGQTFNPCEFVNACDKGHFCATPGVAPMDCDAN
                     ATGCCLPFCDTTEMPASCPGTGLECVPWYEEGQAPPDLATVGLCMIPMP"
     gene            complement(1954..>2927)
                     /locus_tag="VGB85_25995"
     CDS             complement(1954..>2927)
                     /locus_tag="VGB85_25995"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0137574.1"
                     /translation="PRGRAPGLDQFDEDDLEDMSETPAPERVDPARLTAEQREVLTAM
                     AGSRELWTVGNLFYVLRSHGIPTERAELCALLGREHVRDEAAELASQARFHRVHMHDP
                     IEAASLLRRAVELAPGDAALWQQLAQALLAAADPNEALLAVDRALAIQPGLAAAHFER
                     GLVVVHVDPGAAAEAFARAAELGHQPLQARTNHATCLAMIGRRDEALQILERLTGEHP
                     EFAEGWYSLGLAQLKLGRLDLAAHTLGRAIDLAPEHANAHYARACAHALAGAAAPAIT
                     DIARAVAIDPDLRAAIRDDADFAAITDDPEFLRLTAGEARADEALRN"
BASE COUNT          421 a          987 c         1111 g          408 t
ORIGIN      
        1 gggctgccgg ccgactgcgc gagcgaggtg tgcacggcgg gcatgtgcgc gccggccgga
       61 ccgctgtgcg cgcaacagcc ggcggacccg gtcaccggcc agcgctgccc gctgttcatg
      121 gcgtgcacgc agtcgagcga gtgcggcgac ttcaagggct gtcagcagtg gttctgcaac
      181 aacgccaagc gctgcgagct caacgcgctg gtcggctgct ggaccgacaa gggcggggcg
      241 tgcaacgccg acgtcgtgtt cacgcagcag accgcgccgc cggtcgacaa gcgcttcgtg
      301 ccgccggaca acgtcgactt ccgcgagctg gcctcgctgg cgttcacgat caagaacaac
      361 agcgcgagcg acctgtacct cgacaagctg ccgctggtgc tggatgtgat cggcggcggg
      421 tcgaagtttg atgtcgcgtc ggtcaagatc ttcgacaact ccggcggcac cgagcacggc
      481 ggcggcgacc tgctggtgtg cctgacggcc aacccgttct cgttcccggc caacggcgtg
      541 atgggcccgt gcggcggcag tgccttcgcc aagatcccca aggggcagag caaccagttc
      601 atcgtcaacg tcgcgttcgc caaggagaag acgttcatcg ccggccgcgg ctatcgcctg
      661 cgcctcgaca gcacgacggg gatcgagctc aaaaacggct tcaacggacc ggtgtaccag
      721 ggcagcatgt gcggggtgcc gccaggcggc tatgccggcg cgtgggtgac ggcgcagaac
      781 ccgtgaggcc cgtggtcgcg gtgggacctc gatggtgcgc gccgcgggac gttgttctgc
      841 ggcggcaccg ggcggatccc ggaggcgcag cgcgacggca cgccggcttg ttgtcgtttc
      901 cgctctgccc gtggtatcgc tcgaactgcg cgcttctcga ggtatcaccg catgcgatcg
      961 accctgctca agctctgtct cctccccacg ctcgtcgctc tcgcgggctg cggcaaggac
     1021 tccgaaaaca tgacgatggc caccgcgccc ggggccgaga ccaccggtgg tgacgggact
     1081 accagcggcg gacagtcgga gaccggcgcc gtggtgaccg gcgagccgac gaccaccggc
     1141 gcgcagcaga cgacgacggg ggccgagacc tcggcggcga acaccggcga ctcctcgggc
     1201 tcgacgggct cgagcttcct gaccccgccc gacggcggct cggtcaacga tatggagtgc
     1261 agcgtgtggg accaggactg tcccgtcgac cagaagtgca tgccgtgggc caacaacggc
     1321 agcaccgcgt ggaacgccac caagtgcgtg ccggtcgacc cggccaaggg tcagccgggc
     1381 gacacgtgca cggtcgaggg cagcgcggtc agcgggctcg acacctgcga cctcggcgtg
     1441 ctgtgctggg acgtcaagcc cgcgacgatg atgggcacct gcgtcgcgca gtgcaagggt
     1501 ccggagagcg acccgacctg cgacgccaac agcagctgct tcatctccaa cgatggcgtg
     1561 ctgacgttgt gcctgcccaa gtgtgacccg ctcacgcagg actgcccgaa cgagaacctg
     1621 tgcatcccga acccgcagaa ccaggaggag ttcacgtgcg tgctcgatgc ctcgggcgac
     1681 ggggggcaga ccttcaatcc gtgcgagttc gtcaacgcgt gcgacaaggg ccacttctgc
     1741 gcgacgcccg gggtggcccc gatggactgc gacgccaacg cgaccggctg ctgcctgccg
     1801 ttctgcgaca cgaccgagat gcccgcctcg tgcccgggca ccggcctcga gtgcgtgccg
     1861 tggtacgagg agggccaggc cccgccggac ctcgccacgg tcggcctgtg catgatcccg
     1921 atgccgtgag ggcccttcgg gggtcgtcgc gggtcagttg cgcagcgcct cgtcggctct
     1981 ggcttcgcca gcggtgaggc gcaggaactc ggggtcgtcg gtgatcgcgg cgaagtcggc
     2041 gtcgtcgcgg atcgcggcgc gcaggtcggg gtcgatcgcc acggcgcggg cgatgtccgt
     2101 gatcgcgggg gcggccgcgc cggcgagcgc gtgggcgcag gcgcgggcgt agtgggcgtt
     2161 ggcgtgctcg ggggcgaggt cgatcgcgcg gccgagcgtg tgcgcggcga ggtcgaggcg
     2221 gccgagcttg agctgcgcga ggccgaggct gtaccagccc tcggcgaact cggggtgctc
     2281 gccggtcagg cgctcgagga tctgcagggc ctcgtcgcgg cggccgatca tcgccaggca
     2341 ggtcgcgtgg ttggtgcgcg cctgcagcgg ctggtggccg agctcggcgg cgcgggcgaa
     2401 ggcctccgcg gccgcgccgg ggtcgacgtg cacgacgacg aggccgcgct cgaagtgcgc
     2461 ggccgccagc ccgggctgga tcgccagcgc ccggtcgaca gcgagcagcg cctcgttcgg
     2521 gtcggcggcg gcgagcaggg cctgcgcgag ctgctgccac agcgcggcgt ccccgggggc
     2581 gagctcgacg gcgcggcgca gcaagctcgc ggcctcgatc gggtcgtgca tgtgcacgcg
     2641 gtgaaagcgg gcctgcgagg cgagctcggc cgcctcgtcg cgcacgtgct cgcggccgag
     2701 cagcgcgcac agctcggcgc gctcggtcgg gatgccgtgg ctgcgcagca cgtagaacag
     2761 gttgccgacg gtccacagct cgcgcgagcc ggccatcgcg gtcaggacct cgcgctgctc
     2821 ggcggtgagg cgggccgggt cgacgcgctc cggggctggc gtttcggaca tgtcctcgag
     2881 gtcatcctcg tcgaactggt cgaggccggg cgcacggccg cgcggcg
//
LOCUS       DASWNJ010001567         1908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_28175, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001567 DASWNJ010000000
VERSION     DASWNJ010001567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28175"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VGB85_26000"
     CDS             <1..298
                     /locus_tag="VGB85_26000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137575.1"
                     /translation="HHTDFFILTAPSVLAPTDAAPYFGVVDQGLSSFISNTILRWEYL
                     PGSFLFVAYTHRTSLSEGGMRVTFRPGAGFGNLVAPGAQNEDIIFIKLQHLFGL"
     gene            298..681
                     /locus_tag="VGB85_26005"
     CDS             298..681
                     /locus_tag="VGB85_26005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4267 domain-containing protein"
                     /protein_id="HEY0137576.1"
                     /translation="MPDSLHHLAWAFAAGRIILGLGPFLAAGPLIRLLGFPPAHDNPS
                     ARLMARLFGVRDIGLGVLIIGCATDPTLLRAAVLFNLLHDLGDLVAVAIPLIRRQGID
                     RGALMTGTFALGGALAWTGLYLLLP"
     gene            725..1258
                     /locus_tag="VGB85_26010"
     CDS             725..1258
                     /locus_tag="VGB85_26010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137577.1"
                     /translation="MTGPAPRLRDRLCVAAVLLAWHLPVVYHGLVSARPLPGVPWNLH
                     RCHDITCLFDDKPVARHHYYVQVRRIGRPDWQTLDVSELSPMVPFGYRSRIERLLTMW
                     GEKSGLAHRELASWLFRRHRELHPEQPQLTELRFLWTWVLLGEDPLPQGAARPPPAES
                     FPFDRKRVLSVHLPEDR"
     gene            1255..>1908
                     /locus_tag="VGB85_26015"
     CDS             1255..>1908
                     /locus_tag="VGB85_26015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137578.1"
                     /translation="MTLAQRWQRYWYAPIPAIRLDAFRQALLFSLVLYMLHRYMHASE
                     WLSTAGFHPSAAADRYNAPQLPLLSPGMIPLFGLGLFGSLALALFGWLRRYATWLAFF
                     LVVYATYADPIAAFTLNRIYCFSLLILAIAPSPSPAGPDGEPTMIAWPVRLLQLQLLL
                     HYCASGLCKAIQGRWLTDSDLLWFQMQEIYMTDAAAYLVRTLPPWAFTAQQALALGFE
                     "
BASE COUNT          233 a          777 c          565 g          333 t
ORIGIN      
        1 gcaccacacc gacttcttca tcctgaccgc gccgagcgtg ctggccccga ccgacgcggc
       61 gccctacttt ggcgtcgtcg accagggcct gtcgagcttc atctccaaca cgatcctccg
      121 ctgggagtac ctgcccggca gcttcctgtt cgtcgcctac acccaccgca cctcgttgtc
      181 ggagggcggc atgcgggtga cctttcggcc aggcgccggc tttggcaacc tcgtcgcccc
      241 cggcgcgcag aacgaggaca tcatcttcat caaactccag cacctgttcg ggctgtgatg
      301 ccggactcgc tccaccacct cgcctgggcc ttcgccgccg gccgcatcat cctcggcctc
      361 gggcccttcc tcgccgccgg cccgctgatc cgcctgctcg gcttcccgcc agcgcacgac
      421 aacccgagcg cgcgcctgat ggcccggctg ttcggcgtcc gcgacatcgg cctcggcgtt
      481 ttgatcatcg gctgcgcgac ggacccgacc ctgctgcgcg ccgcggtcct gttcaacctc
      541 ctccacgacc tcggcgacct cgtcgcggtg gcgatccccc tcatccgtcg ccagggcatc
      601 gaccgcggcg ccctgatgac cggcaccttc gccctcggcg gcgcgctcgc ctggaccggc
      661 ctctacctgc tcctgccgtg agccacggct cgacaagcgg cgggcatggt ggcaagaaac
      721 ctcggtgacc ggccccgcac cccgcctgcg cgaccgcctg tgcgtcgccg cggtcctgct
      781 cgcctggcac ctgcccgtcg tctaccacgg cctcgtctcg gcgcgcccgc tgccgggcgt
      841 gccgtggaac ctgcaccgct gccacgacat cacctgcctg ttcgacgaca agccggtcgc
      901 gcgccaccac tactacgtgc aggtccgccg gatcggtcgc cccgactggc agaccctcga
      961 cgtcagcgag ctgtcaccga tggtcccgtt cggctaccgc tcgcggatcg agcgtctgct
     1021 gaccatgtgg ggcgagaagt cgggcctcgc ccaccgcgag ctcgccagct ggctgtttcg
     1081 ccgccaccgc gagctgcacc ccgagcagcc gcagctgacc gagctgcggt tcttgtggac
     1141 atgggtgctg ctcggcgagg acccgctgcc gcagggcgcc gcgcgcccgc cccccgccga
     1201 gtcgttcccg ttcgaccgca agcgcgtgct ctccgtgcac ctccccgagg accgatgacg
     1261 ctcgcgcaac gctggcagcg ctactggtac gcgccgatcc ctgcgatccg cctcgatgcg
     1321 tttcgtcagg cgctgctgtt tagcctcgtg ctctacatgc tgcaccgcta catgcacgcg
     1381 agcgagtggc tgagcaccgc cggctttcac ccctccgccg cggccgaccg ctacaacgcg
     1441 ccgcagctcc cgctgctgag ccctggcatg atcccgctgt tcggcctcgg tctgttcggc
     1501 agcctcgcgc tggcgctgtt cggctggctg cggcgctacg cgacctggct ggcgttcttc
     1561 ctcgtcgtct acgcgaccta cgccgatccg atcgccgcgt tcaccctcaa tcgcatctac
     1621 tgcttctcgc tcctcatcct cgccatcgcc ccctcgccat cccccgccgg ccccgacggc
     1681 gagccgacga tgatcgcctg gccggtgcgc ctgctgcagc tccagctgct cctgcactac
     1741 tgtgcatccg gcctgtgcaa ggcgatccag ggccgctggc tgaccgacag cgacctgctg
     1801 tggttccaga tgcaggagat ctacatgacc gacgcggccg cgtacctggt ccgcacgctg
     1861 ccgccgtggg ccttcaccgc ccagcaggcg ctcgccctcg gcttcgag
//
LOCUS       DASWNJ010001568         2629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_521361, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001568 DASWNJ010000000
VERSION     DASWNJ010001568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521361"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="VGB85_26020"
     CDS             <1..1225
                     /locus_tag="VGB85_26020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137579.1"
                     /translation="HPNAQQRKGVVDKDHPDGLHPIAPKSLPITLGDVVYNFETLPTR
                     FGPVGEHPGFVIAASGITKGEDAFKMTVRVNLNALPYKGVDLTSSHVASVNSTDSQIE
                     NVFDFSDPDWMRIEGLKDTLEIAEMTVRILENDAFIPGGDVREPTPLGNSPAWDLPDW
                     EFERLIIEMARRRTNLIGAHCDDYELGTGVQAFSACIDMTGWTEMKTFTDIGNPPPPA
                     YFWDVLAEVAQVRLHDPASPSDPPIAEGAADVEFTLRNVQIPLDEVALLKGIQKNFAA
                     NPRALGDMAELLNNNAEGDADFYYYRPDASNGADIEGDYLYFVTEDDLRTDAEGALVR
                     PYAYAHPGFYADQGLTTKLSTTAAIDGDDTHEKLKVTPGQTVYTEDDAGQVYRVVVGE
                     KTVRSVLSLELTRVR"
     gene            1296..>2629
                     /locus_tag="VGB85_26025"
     CDS             1296..>2629
                     /locus_tag="VGB85_26025"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEY0137580.1"
                     /translation="MPRTSCNPRMPSLLRPRALPAPATRLVGALALALPLALAMTLAP
                     SAAWASGLDAPMVGSGQSGPTTRDGAAIHWNPANLAYMKKGEFFGGLMLVVGDIRYQR
                     DYRSTYQTPDTLEFKSPISPENVDPSKMGFGREVKANPVAPLGNIFVVLPPIKNRLVF
                     GLGLYVPYGAPLKFDRNGPQAQQLQQAFIVASHITASAGVRLHDMVSLGAGISYVLGF
                     AELAKKQDFGSVAEFGAGLDKLGQTNSFGPNAPSDVRELDTLSRPIALKKAFSHGVSF
                     NVGVTVNPTKKLTLTLSYQHGAHMRYQGKFALDMNDPFFTQDLAAQGLKFKPLVQGDA
                     TLEFKLPKRITLGAAYQINERWRVDGFFQYVTYSEVKEFKVSTSSPDLAQPALGIGTD
                     LAVLLPRNWKNTVWVEGNVRFRPTERLLTSVTLGYQSPASPDKTVDTASPDG"
BASE COUNT          454 a         1052 c          752 g          371 t
ORIGIN      
        1 gcacccgaac gcccagcagc gcaagggtgt cgtcgacaag gaccacccgg acggcctgca
       61 cccgatcgcc cccaagtcgc tgccgatcac cctcggcgac gtcgtctaca acttcgagac
      121 cctgccgacc cgcttcggcc cggtcggcga gcaccccggc ttcgtgatcg ccgcgagcgg
      181 catcaccaag ggcgaggacg cgttcaagat gaccgtgcgc gtcaacctca acgccctccc
      241 ctacaagggc gtcgacctga ccagcagcca cgtcgccagc gtcaacagca ccgacagcca
      301 gatcgagaac gtcttcgact tcagcgaccc ggactggatg cgcatcgagg gcctcaagga
      361 caccctcgag atcgccgaga tgacggtccg gatcctcgag aacgacgcct tcatccccgg
      421 cggtgacgtc cgcgagccga ccccgctcgg caactcgccc gcgtgggacc tgcccgactg
      481 ggagttcgag cgcctgatca tcgagatggc ccgccgccgc accaacctga tcggcgccca
      541 ctgcgacgac tacgagctcg gcaccggcgt tcaggcgttt agcgcctgca tcgacatgac
      601 cggctggacc gagatgaaga cctttacgga catcggcaac ccgccgccgc cggcctactt
      661 ctgggacgtg ctcgccgagg tcgcgcaggt ccgcctgcac gaccccgcct cgccctccga
      721 cccgccgatc gccgagggcg cggccgacgt cgagttcacc ctgcgtaacg tacagatccc
      781 gctcgacgag gtcgccctgc tcaaggggat ccagaagaac ttcgccgcca acccgcgcgc
      841 gctcggcgac atggccgagc tgctcaacaa caacgccgag ggcgacgccg acttttatta
      901 ctaccgcccc gacgccagca acggcgccga catcgagggc gactacctct acttcgtcac
      961 cgaggacgac ctgcgcaccg acgccgaggg cgccctcgtg cggccctacg cctacgccca
     1021 ccccggcttc tacgccgacc agggcctgac gaccaagctc tcgacgaccg ccgcgatcga
     1081 cggtgacgac acccacgaga agctcaaggt gaccccgggt cagaccgtct acaccgagga
     1141 cgacgccggc caggtctatc gcgtcgtcgt cggcgagaag accgtgcgca gcgtcctctc
     1201 gctcgagctg acccgcgtcc gctgataccc accctcgcat ccccctcgca tcccccgcgc
     1261 gcccccctgc tctctacacg ccccgccccg cgcccatgcc ccgcacctcc tgcaaccccc
     1321 gcatgccctc tctcctgcgt cctcgcgccc tcccggcacc cgccacgcgc ctcgtcggcg
     1381 cgctcgcgct ggccctgccg ctcgcgctgg ccatgacgct cgcgccctcg gccgcgtggg
     1441 cctcgggtct ggatgcgccg atggtcggca gcggacaaag cggcccgacg acgcgcgacg
     1501 gcgccgcgat ccactggaac ccggccaacc tcgcgtacat gaagaagggc gagttcttcg
     1561 gcggcctgat gctcgtcgtc ggcgacatcc gctaccagcg cgactatcgc agcacctatc
     1621 agacccccga caccctcgag ttcaagtcgc cgatctcccc ggagaacgtc gacccgagca
     1681 agatgggctt cggccgcgag gtcaaggcca acccggtcgc gccgctgggc aacatcttcg
     1741 tcgtgctccc gccgatcaag aaccgcctgg tgttcggcct cggcctctac gtgccctacg
     1801 gcgccccgct caagttcgac cgcaacggac cgcaggccca gcagctgcag caggccttca
     1861 tcgtcgcctc gcacatcacc gccagcgccg gtgtgcgcct gcacgacatg gtctcgctcg
     1921 gcgccggcat ctcgtacgtg ctcggcttcg ccgagctcgc caagaagcag gacttcggct
     1981 cggtcgcgga gttcggcgcc ggcctcgaca agctcggcca gaccaacagc ttcggcccca
     2041 acgcgccgag cgacgtccgc gagctcgaca ccctctcgcg cccgatcgcc ctcaagaagg
     2101 ccttctcgca cggcgtcagc ttcaacgtcg gcgtcaccgt caacccgacc aagaagctga
     2161 ccctgacgct gtcgtaccag cacggcgcgc acatgcgcta ccaaggcaag ttcgcgctcg
     2221 acatgaacga cccgttcttc acccaggacc tcgcggccca gggcctcaag ttcaagccgc
     2281 tggtccaggg tgacgccacc ctcgagttca agctgcccaa gcggatcacg ctcggcgccg
     2341 cttaccagat caacgagcgc tggcgcgtcg acggcttctt ccagtacgtg acctacagcg
     2401 aggtcaagga gttcaaggtc tcgaccagct cgccggacct cgcacagccc gcgctgggca
     2461 tcgggaccga cctcgccgtg ctgctcccgc gaaactggaa gaacaccgtg tgggtcgagg
     2521 gtaacgtccg cttccgcccg accgagcgcc tgctgacctc cgtcaccctc ggctatcagt
     2581 cgcccgcctc gcccgacaag accgtcgaca ccgcctcgcc cgacggtca
//
LOCUS       DASWNJ010001569         2741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_205597, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001569 DASWNJ010000000
VERSION     DASWNJ010001569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205597"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VGB85_26030"
     CDS             complement(<1..653)
                     /locus_tag="VGB85_26030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137581.1"
                     /translation="MNALWRKLAEYELDEAIADATALHAELRAEVQPDREQAGWAFYS
                     LFKALYTAGRTAEAHALLITDEDGRDHEIAPVNRAWMYSVGAECAIHRDNAAELVRLG
                     QKCLAMRVALADLDSALQCASSMCTLLMARNLFSHNGPFARFLVQHAGEAGRWDLVGE
                     GISQLQWNGTQTRDDRVLGEAAAALERLAAVTVEDPEEQAAIDAVVADQRAVVGSLQ"
     gene            857..1771
                     /locus_tag="VGB85_26035"
     CDS             857..1771
                     /locus_tag="VGB85_26035"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEY0137582.1"
                     /translation="MPSQLSRADWDLLLARLDAGACTPFLGPGVHDGRTPLPGDIAAQ
                     WAETGGYPLEDRWNLARVAQFMAIERGPRWPQDELASALAETRATPEGESAETLVSLA
                     DLPFRTYVTTAADDRLVRALRSRTCERAGKQVSRRPRADFCRWQEALRSAALATPFDE
                     DPRYVPSVEEPFVFHLHGQVAVRQSLVVTEDDHIDLLLATARYPNLIPPAVQRALRGG
                     PLLLLGQPLAEWGFRALLRSLCGPDFGGLQAGSVAVQAAPPGAGEEVLGYVEKLFGKL
                     RIAVYWGTPQEFLQELRERRDGTHSGEQ"
     gene            1785..>2741
                     /locus_tag="VGB85_26040"
     CDS             1785..>2741
                     /locus_tag="VGB85_26040"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0137583.1"
                     /translation="MQSAETTSPYPGPRPFTAAERDRFFGRTRELQDLAALVIAHPVT
                     VLYGPTGAGKTSLICAGVIPELERAGFDVMPLARVGGLLPPGTDAARLRNVFTYSVLL
                     AWAPDGADEDALARHTLASYLRAMPGVDDGVRRPRVIALDQLEDLFTAHPAQWEQREA
                     FLRELAECLRSSSERSDRNDRPMASPSSAGQSDPPMRLLLAIRDDRLADLERHADLFP
                     DSLRARLPLDGLRVPAAMEAISGPASNLFSGTDAEQLARNLAQRRVRLANGKLALVPS
                     DRVEPMQLQLACDERFQRGGRSGAMAKPRDPDEALTRFYDLAV"
BASE COUNT          371 a         1048 c          937 g          385 t
ORIGIN      
        1 ttttgcaggc tgccgacgac cgcgcgttga tcggcgacca ccgcgtcgat cgccgcctgc
       61 tcctcgggat cttccaccgt caccgcggcg agccgctcga gcgccgccgc ggcctcgccg
      121 aggacgcggt cgtcccgggt ctgcgtgccg ttccactgca gctgcgagat gccctcgccg
      181 acgaggtccc agcggccggc ctcgccggcg tgctggacca ggaagcgcgc gaacgggccg
      241 ttgtgactga acaggttgcg ggccatcagc agcgtgcaca tcgagctggc gcattgcagg
      301 gccgagtcga ggtctgccag cgcgacgcgc atagcgaggc atttttggcc gaggcgcacc
      361 agctccgcgg cgttgtcgcg gtggatcgca cactcggcgc cgacggagta catccaggcg
      421 cggttgaccg gggcgatctc gtgatcgcgg ccatcttcgt cggtgatcag cagggcgtgg
      481 gcctcggcgg tgcggccggc ggtgtagagc gccttgaaca gcgaatagaa ggcccaaccg
      541 gcctgttcac ggtccggctg gacctccgcg cgcagctcgg cgtgcagcgc cgtcgcgtcg
      601 gcgatcgcct cgtccagctc gtactccgcc agcttgcgcc acagggcgtt catacgcccc
      661 gcgaaagtct ctccctgcat aacccctccg gatcccctcg accagcgccc gcagtgtcac
      721 acgaagccgc ggctgtttgg gcgcgccgcc tgtgaccccg gcctatcccc ggacgaccgc
      781 gagctattcg agctgaacat gcgaaaggtt tcgcggacgc ccgtccgcgt gtaacgtggg
      841 cgatggagcg gaccccatgc catcgcaact atcacgggcg gattgggacc tgctgctcgc
      901 gcggctggat gctggcgcgt gtacgccgtt tctcgggccg ggtgtgcacg acgggcggac
      961 ccctctgccg ggtgacatcg ccgcccagtg ggccgagacc ggcggctacc cgctggaaga
     1021 ccgctggaac ctcgcccgcg tcgcgcagtt catggccatc gaacgcggac cacgctggcc
     1081 gcaggacgag ctcgcctcgg cgctcgccga gacccgggcc acgcccgagg gcgagtccgc
     1141 cgagaccctc gtcagtctgg ccgacctgcc ctttcggaca tacgtgacca ccgccgccga
     1201 cgaccgcctg gtccgcgcgc tgcgcagccg cacctgcgag cgcgccggca agcaggtgtc
     1261 ccggcggccg cgcgccgact tctgccgctg gcaggaggcc ctgcgctccg cggcgctggc
     1321 gacccccttc gacgaggacc cgcgctacgt gccctccgtc gaggagccgt tcgtgttcca
     1381 cctgcacgga caggtcgcgg tgcgtcagtc gctcgtcgtc accgaggacg accacatcga
     1441 cctgctgctg gcgaccgcgc gctacccgaa cctgatcccg ccggcggtcc agcgagcgct
     1501 gcgcggcggc ccgctgctgc tgctcgggca gccgctcgcc gaatggggct ttcgcgccct
     1561 cctgcgcagc ctgtgtggcc ccgacttcgg cggcctgcaa gcgggcagcg tcgcggtcca
     1621 ggccgccccg ccgggcgctg gcgaggaggt gctcggctac gtcgagaagc tgttcggcaa
     1681 gctgcgcatc gccgtctact ggggcacccc ccaggagttc ctgcaggagc tgcgcgagcg
     1741 ccgggacggc acgcacagcg gagagcaata gccgggagac acgcatgcag tccgccgaga
     1801 ccacgagtcc atacccggga ccgcgcccgt tcaccgccgc cgagcgcgac cgcttcttcg
     1861 gccgcacacg cgagctccag gacctcgcgg cgctggtgat cgcccacccg gtcacggtct
     1921 tgtacggccc gaccggggcc ggcaagacct cgctgatctg cgccggtgtg atccccgagc
     1981 tcgagcgcgc cggcttcgac gtcatgccgc tcgcgcgcgt cggcggcctg ctgccgcccg
     2041 gcaccgatgc ggcccgcctg cgcaacgtct tcacctactc cgtgctgctg gcatgggcgc
     2101 ccgacggcgc cgacgaggac gcgctcgcgc gccacaccct cgcctcgtac ctgcgtgcga
     2161 tgcccggggt cgacgacggc gtgcggcgcc cgcgggtgat cgcgctcgat cagctcgagg
     2221 acctgttcac cgctcatccg gcgcagtggg agcagcgcga ggcgttcctg cgcgagctcg
     2281 ccgagtgcct gcgcagcagc agcgagcgca gcgaccgtaa cgaccgtccc atggccagcc
     2341 cgagcagcgc cggccagagc gacccgccga tgcgcctgct gctggcgatc cgcgacgacc
     2401 ggctcgccga cctcgagcgg cacgccgacc tgttccccga cagcctgcgc gcgcgcctcc
     2461 ccctcgacgg cctgcgcgta cccgcggcga tggaggcgat ctccgggccc gcgagcaacc
     2521 tgttctccgg gacagacgcc gagcagctgg cccgcaacct cgcccagcgg cgcgtgcggc
     2581 tcgccaacgg caagctcgcg ctcgtcccct cggaccgcgt cgagccgatg cagcttcagc
     2641 tcgcctgcga cgagcggttt caaaggggcg gccgcagcgg cgcgatggcc aagccgcgcg
     2701 accccgacga ggccctgacc cgcttctacg acctcgcggt c
//
LOCUS       DASWNJ010001570         3919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_276078, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001570 DASWNJ010000000
VERSION     DASWNJ010001570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276078"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1183)
                     /locus_tag="VGB85_26045"
     CDS             complement(143..1183)
                     /locus_tag="VGB85_26045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137584.1"
                     /translation="MTMKRGRSSLLVVALAGGCGGNDGGMTDGGGSSTGTTATSSDTG
                     ETTVVDPTEDVDTGSSSTSGEAQHPAVAAFWAAFLAEDYGALDQVSADLDAAAAEMSD
                     DPEVVLSAALAHLWRLAEFERDPDQGLSVQIGSSMAALAGLKAAKALAPEDPRIPCYL
                     GLLMIDTGEAIGDQQMAAQGHAEIDAAIEGFPEFSLFCRMLEYGDAPRDSPEFQQAVD
                     AMWTSLDLCYPMVDPSLPDVSSYLDQQTDQGIKRVCWNLVPKAMHNWEGYFLHAGDII
                     AKTGDVEVATVLYENAMLLDWDSWLHQDLIDQRLADVAARADSYQDADPANDAAVGSG
                     RYSCTMCHGGST"
     gene            1346..1693
                     /locus_tag="VGB85_26050"
     CDS             1346..1693
                     /locus_tag="VGB85_26050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEY0137585.1"
                     /translation="MTGEPSNSTRLVRPNRLLPTRACWISALPALPFLEDCLAVTSPA
                     DPGASGSFAVEFKVGAGGRGKDPVVQRSTLAETEATDCVTRMIEHLKFPAPVATDPVS
                     VTASSTLSVDSGP"
     gene            complement(1915..2931)
                     /locus_tag="VGB85_26055"
     CDS             complement(1915..2931)
                     /locus_tag="VGB85_26055"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0137586.1"
                     /translation="MLHPAEQSEAPEIEEVMAFLRVAETGSFTAAGTRLGIPKSTVSR
                     RVARLEDKLGVQLLHRTTRSLALTEAGALYNERAGRALASLDEATLAAREGRETARGH
                     LRLTAPYDVGAGSLADIIAAFTRAYPEVTVEVVLSDRTLDLVAEGLDLGLRGAAALPD
                     SSLVARKLASLKFQLVASPAYVRAHGAPTTPAELAGHELALLRATGGRGKLQLVGKDG
                     ASEELSVRAAVSSNGFGFLRSAALAGGYIAILPDIHAAADLRTGRLVRVLPDYTVGDG
                     NVYLVHPAAKVLPAKARVFRDFLQANIELFMHGPECVEAKAQHARATAARSERRPPRS
                     TRPA"
     gene            2987..3424
                     /locus_tag="VGB85_26060"
     CDS             2987..3424
                     /locus_tag="VGB85_26060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137587.1"
                     /translation="MTRIAPVARILLGLVFLVFGLNGFFHFLPMPPLGERPGAFMGAL
                     AATGYMFPLIKGTEVVAGALLLAGRFVPLALLLLAPIVVNILAFHLVLTPGDVGMAVV
                     LTALTAFLGWAYRDSFRGVLDPNAVPTAATESAPHPSAFPQRA"
     gene            complement(3429..>3919)
                     /locus_tag="VGB85_26065"
     CDS             complement(3429..>3919)
                     /locus_tag="VGB85_26065"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0137588.1"
                     /translation="RVAAYVRDIQAVQPHGPYRLIGYSYGGYPALGVAAALEAAEAQY
                     APALTIVEGAGGLWVPMPGGTWQPAWISGLAATPVVVGRLGLGTINHSLLTIATLRAL
                     GLPPCGFILTQNMVGSDLSYAHNERVVAAASGLPCLGVLAFGTGPEEHGWVRPEAWPL
                     LA"
BASE COUNT          563 a         1526 c         1238 g          592 t
ORIGIN      
        1 cgagccggct gacgcggtac ttgcacacga gatcctgaat caccagcacc ggcaccaaca
       61 gcaccagcaa cccgagcttg tacagccagc gcatcgccac aatgtgccac ggccgacagc
      121 gagagacaaa ggccgcccgc gattacgtcg acccgccgtg gcacatcgtg cagctgtacc
      181 ggcccgagcc gaccgccgcg tcgttcgccg gatcggcgtc ctggtacgag tcggcgcgcg
      241 cggcgacatc cgcgagacgc tggtcgatca ggtcctggtg cagccagctg tcccagtcga
      301 gcagcatcgc attctcgtac agcacggtcg ccacctcgac atcgccggtc ttggcgatga
      361 tgtcgccggc gtgaaggaag tagccctccc agttgtgcat ggccttgggt accaggttcc
      421 agcacacacg cttgatcccc tgatcggtct gctgatcgag gtaggacgag acatcgggca
      481 ggctcgggtc gaccatcggg tagcagaggt cgagcgacgt ccacatggcg tcgaccgcct
      541 gctggaactc gggtgaatcg cggggcgcgt ccccgtattc gagcattcgg caaaacaggc
      601 tgaattcggg gaagccctcg atcgccgcgt cgatctccgc gtgtccctgc gcggccatct
      661 gctggtcgcc gatcgcctca ccggtgtcga tcatcaagag gccgagatag cacgggatcc
      721 gggggtcctc cggcgcgagc gccttcgcgg ccttcagacc cgcgagcgcc gccatcgacg
      781 acccgatctg cacgctgagc ccctggtccg ggtcgcgctc gaactcggcg agccgccaca
      841 ggtgggcgag cgccgcgctc agcacgacct cggggtcgtc ggacatctcg gcggccgcgg
      901 cgtcgaggtc cgcgctgacc tggtcgagcg caccgtagtc ctccgccaga aacgcggccc
      961 agaacgctgc gacggccggg tgctgcgcct ctccgctcgt gctcgagctg ccggtgtcga
     1021 cgtcctcggt cgggtcgacg accgtcgtct cgcccgtgtc gctgctcgtc gcggtcgtgc
     1081 ccgtcgagct gccgccgccg tccgtcatcc cgccgtcgtt cccgccgcac ccgcccgcga
     1141 gcgcgacgac cagtaggctg ctccgacctc gcttcatcgt catgcccgca gggtacccgc
     1201 gtcacgacca tgcgtacagg tcgcggccgc agtttggacc gtcgaacggt gcccaccaca
     1261 tcgttggctc gcgcaccttt ctgccccaaa aagtcggcgt cacctgttcg agacgctgca
     1321 ctcgatctcg tcgccggact tgaatttgac gggcgagccg tcgaactcga cgaggctggt
     1381 gcggccgaat cggctgttgc cgactcgcgc ctgctggatc tcggcgctgc cggctctgcc
     1441 gttcctcgag gattgcctcg ctgtcacatc tcccgccgac ccgggagcga gcggctcgtt
     1501 cgccgtcgaa ttcaaggtcg gcgccggagg ccgggggaag gaccccgtcg tgcagcgctc
     1561 gaccctcgcc gagaccgagg ccacggattg cgtgacgcga atgatagaac atctcaaatt
     1621 cccggccccc gtggccaccg acccggtctc cgtgaccgcg tcatccacac tgagcgtcga
     1681 ttcggggccg tgaccgcccg gtcgacggct cggcgcccga ggtcgtcgaa gtcccgccgt
     1741 catcgccaca gcccgccacc gcgaggacca ccgccgccgc gaagatccgc cccagatgat
     1801 ccgtacctac ttgatgcatc ccgcgatagg acacgctcac gccgatgccg gcaaccgcga
     1861 agatcgtccc gatgccgacg tcggcacgag ctcggcgtcg cgtccgcacc gcgttcacgc
     1921 cggacgcgtc gagcgcggcg gacgacgctc gctgcgcgcc gccgtcgcac gcgcgtgctg
     1981 ggccttcgcc tcgacgcact cgggcccgtg catgaacagc tcgatgttcg cctgcaggaa
     2041 gtcgcggaac acccgcgcct tcgccggcaa caccttcgcc gccgggtgca ccagatacac
     2101 gttgccgtcg ccgaccgtgt agtccggcaa cacccgcacc agccgacctg tgcgcaggtc
     2161 cgccgccgcg tggatgtccg gcaagatcgc aatgtacccg cccgcgagcg ccgccgagcg
     2221 caggaagccg aagccgttcg agctgaccgc cgcgcgcacg ctcagctcct cgctcgcgcc
     2281 gtccttgccg acgagctgca acttcccccg accacccgtc gcccgcagca gcgcgagctc
     2341 gtggcccgcg agctccgccg gcgtcgtcgg tgccccgtgc gcccgcacgt acgccgggct
     2401 cgccaccagc tgaaacttca ggctcgccag cttgcgcgcc accagcgacg agtccggcag
     2461 cgccgccgcc ccgcgcagcc cgaggtcgag cccctcggcg accaggtcga gcgtgcggtc
     2521 ggacagcacg acctcgaccg tgacctccgg gtacgcgcgc gtgaacgcgg cgatgatatc
     2581 cgccagcgag cccgcgccga cgtcgtaggg ggccgtcagt cgcaggtgtc cgcgggccgt
     2641 ctcacgcccc tcgcgcgcgg cgagcgtcgc ctcgtcgagg ctcgccaggg ctcgtcctgc
     2701 ccgctcgttg tacagcgccc cggcctcggt cagcgcgagg ctgcgggtgg ttcgatgcag
     2761 cagctgcacg ccgagcttgt cctcgagccg cgcgacgcga cggctgaccg tcgacttggg
     2821 gatccccagt cgagtgccgg ccgcggtgaa cgatccggtc tcggcgaccc gcaagaatgc
     2881 catcacctcc tcgatctccg gcgcctcgga ctgttccgct gggtgcaaca tctcgtctca
     2941 aactagcatc cttgttcgct gcggcacaac gccacaccat ccacgcatga ctcgcatcgc
     3001 ccccgtcgcc cgcatcctcc tcggcctcgt cttcctcgtg ttcggcctca acggcttctt
     3061 ccacttcctg ccgatgcccc cgctcggcga gcgcccgggc gccttcatgg gcgcgctcgc
     3121 ggccaccggc tacatgttcc cgctgatcaa gggcaccgag gtcgtcgccg gcgccctgct
     3181 gctcgccggc cgcttcgtcc cgctcgcgct gctgctgctc gcgccgatcg tcgtcaacat
     3241 cctcgccttc cacctcgtgc tcacccccgg cgacgtcggc atggcggtcg tgctcaccgc
     3301 cctgaccgcc ttcctcggct gggcctaccg cgacagcttc cgcggcgtgc tcgacccgaa
     3361 cgcggtcccc acggccgcga ccgagtccgc cccgcatccg agcgccttcc cgcagcgcgc
     3421 ctgagagctc acgccagcag cggccacgcc tcgggccgga cccagccgtg ctcctcggga
     3481 cctgtcccga aggccagcac accgaggcac ggcagcccgc tcgccgccgc gaccacccgc
     3541 tcgttgtgcg cgtacgagag atcgctgccg accatgttct gcgtcaggat gaagccgcaa
     3601 ggcggcagcc cgagcgcgcg cagcgtggcg atcgtcagca ggctatggtt gatcgtcccg
     3661 agcccgagcc gaccgaccac caccggcgtc gccgcgagcc cggagatcca cgccggctgc
     3721 cacgtcccgc ccggcatcgg cacccacagg cctccggccc cctccacgat cgtcagcgcc
     3781 ggggcgtact gcgcctccgc ggcctcgagc gccgccgcga cgccgagcgc cgggtagccg
     3841 ccgtacgagt acccgatgag ccggtagggc ccgtgcggct gcacggcctg gatgtcccgg
     3901 acgtatgccg cgacgcgct
//
LOCUS       DASWNJ010001571         6248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_64108, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001571 DASWNJ010000000
VERSION     DASWNJ010001571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64108"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..974)
                     /locus_tag="VGB85_26070"
     CDS             complement(222..974)
                     /locus_tag="VGB85_26070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137589.1"
                     /translation="MLQIDRCSWVDDRTALRHAPPDAVYYPAVVCFGHRYEAVLEGSA
                     SQFSAGVKASFLLFSAGMDAFASQHKLKLNIKADGLRRKKDCSTLTFSDQASFEACFE
                     PAETAPTPVLVEWRVIPGRKPPPGKVAWQATKTADCGGTQVTCRPCQSWRFESLDFSD
                     KGSDFDGDGDVRIVISEPNGAERKFYGSAPKFKDSPIYAAPGDIIRYDAYDEDLLRDD
                     PLGTGFITVQDFHRDGRASQGKLTVIGQCERS"
     gene            complement(1701..2816)
                     /locus_tag="VGB85_26075"
     CDS             complement(1701..2816)
                     /locus_tag="VGB85_26075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137590.1"
                     /translation="MRLLELADLFEQGLPRAAAKADIIESLAHSKRASFGKLLDALKR
                     EELKDICRVHRRAPLHRPCEVHARHPAGFNKDLQRPEGPGLRRPRGLRPPSPAHRTRP
                     RRPPRQRLGRYRGPVLHPPRTPAERLTYEAFEDAVLDRLFALNAERAAEEERQGPRNK
                     SPGKPTPTAPDASAEKRLQNGLQVHHPAPSRQRRQQPMNYEIFGGFEIPRKPGKPAVA
                     DTRDRQAFWKELDEAHPGLSSARGCYVFGIRAGRGILPWYVGKTCKQSFKGECFELHK
                     LRHYDDAISDKKRGTPLLYLVARMTKSGRFATSAPPNEVRFLEHMLIGMALQRNKFLK
                     NIAHTTFLKGLHVPGLVNPGKGPPRGAAAVFKRMVGG"
     gene            complement(2947..3999)
                     /locus_tag="VGB85_26080"
     CDS             complement(2947..3999)
                     /locus_tag="VGB85_26080"
                     /inference="COORDINATES: protein motif:HMM:NF016819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEY0137591.1"
                     /translation="MFTPRPALAHEVGLSRGVYVVEGEVVAAELTFARDELRGIAPAL
                     DTDGDGTLSGPELAAGTGALQVVVTGVQVAGDGQECSGAVVDAALTEGDGARMHLRYA
                     CPAAPRRLELNMPLLATLASGHRHLAEVRRPATDAAPEGVRLAEPVLHSRSATASVEL
                     GEPTAPSRPPVSAYFLIGVEHILIGIDHLVFLLGLVVIGGRLRSLIAVITAFTIGHSL
                     SLALATLGIWTPDPGFVEPAIALSIAYVGIENFLKADAGGRWRITLPFGFVHGFGFAG
                     VLAEIGVPDDAIVSALLLFNLGVEAGQLLVLALVLPLLALLGKSPAYQRVRGPRWISA
                     AIVIAGLYWFVERVLG"
     gene            complement(4247..4603)
                     /locus_tag="VGB85_26085"
     CDS             complement(4247..4603)
                     /locus_tag="VGB85_26085"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0137592.1"
                     /translation="MRLNHLDLQVSDVQKTVAFFTTIFGLELQSNPRSPAIAILGDGE
                     GFVLVLQRRIPVVYPEGFHLGFLVDDVATVHAIHTRARAAGLEISDVDTNNRGTMTYC
                     RAPDGYAVEVACHRPR"
     gene            complement(4800..5819)
                     /locus_tag="VGB85_26090"
     CDS             complement(4800..5819)
                     /locus_tag="VGB85_26090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137593.1"
                     /translation="MRGPYIAGPTYDAVMFIGAPLLALALGIAVAGTPWADEEVVLWG
                     NEGTATSIFIGSFIMAHLALVAVRSHLNRRVFARHPARFIAVPLVLLALLCASDWILA
                     IAVVATVWWDVVHSSLQTFGLGRIYDARRGNPPALGRRLDLGLNLLLYMGPVLAGATL
                     LDHLRPLGALEEVGALAIDQVPTHVATHQPTLAAVLLIGGGLYLLIYALGYLRLHRRG
                     YHVSPQKVALLASTGLCSIYTWGFNSFGEAFFIMNFFHAWQYFGLVWWSERDNLRRHM
                     RLDGVRWGGLAALACMLAAAGAYGVWAEFVSSEQRFALSVTLVVSLMHFWYDGFIWSV
                     RRQEV"
     gene            5975..>6248
                     /locus_tag="VGB85_26095"
     CDS             5975..>6248
                     /locus_tag="VGB85_26095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137594.1"
                     /translation="MKHLPLSLWTLCTLVACDAAVVASAQQDEPAAPAAVELQKVEHS
                     EKAAEDAAAAVKVDADVKATIKPEVLDLESINFLVKKGKVKDAAALE"
BASE COUNT         1038 a         1937 c         2277 g          996 t
ORIGIN      
        1 gcgttcttct cccggcggcg cgcgcggcgt tgagggcgaa gagacggtcg aggacggcgt
       61 cctcgaaggc ctcaaaggta aggcgctcgg ccggggtgcg ggggtgtagg gcggcacctc
      121 aatgtcgtcc cagccgtagg cgtgcaggac ggcgcggtcg agtccgatgt gcagccagtc
      181 gaccatccgg cggggctcgg ccgggagcag cggcccgagc gtcaggagcg ctcgcattgg
      241 ccgatcacag tcagcttccc ttgcgaggcg cggccgtcgc gatgaaagtc ttgaacggtg
      301 atgaaaccag tgcccagggg atcatcgcgc agaaggtcct cgtcgtaggc atcatatcgg
      361 atgatatcgc cgggtgccgc gtagatcgga ctatccttga acttgggcgc ggagccatag
      421 aactttcgtt cggcgccgtt cggttcgctg atcacgatcc ggacgtcgcc gtcgccgtcg
      481 aagtcggagc ccttgtccga gaagtccagc gactcgaacc gccatgactg gcagggacgg
      541 caggtgacct gtgttccgcc gcagtccgca gttttcgtcg cctgccaggc gacctttccg
      601 ggcggcggct tacgtccggg tatgacgcgc cattcgacca agaccggcgt cggggccgtc
      661 tccgccggtt cgaaacaggc ctcgaaggaa gcctgatcgc tgaacgtcag ggtgctacag
      721 tcttttttcc tcctgagtcc gtcggccttg atattgagct tgagtttgtg ctgagaagcg
      781 aaagcatcca tgccagcgga gaacaacagg aacgaggctt tcaccccggc gctgaattgg
      841 ctggcgctgc cctccagcac ggcctcgtag cggtgtccga agcagacgac tgcaggatag
      901 tatacggcgt ccggtggggc gtgacgcagg gcagtacggt catcgaccca gctgcaacga
      961 tcgatctgga gcacgcgatg cgctgcgaat ctcgatttgc ttgcagttcc gccgctcact
     1021 tgcactcccc atgcgcccgc atgcgcgtgc aatgactgct ccgtgtcgat ggtgaagacg
     1081 ctgccttcgt gtcgagatac agggccggtg gcgaccctac ctttcggaaa gacgcttcgc
     1141 ccgtccgggc cgcggtaagc ctggaagttg gcagtatatg gagcccccag gtctcgttcg
     1201 gagaaggcga gcgttggagg cggtgcgaag atcacatcgg tggcagtcgg cggaggggga
     1261 atacggaccg ccactttcac ggtcggggtg cgtgcgcggg tcgagaccct ggagcgagca
     1321 ggccccttcg gcgcgggctt tttttgcgcg acggcgcgtc tttgcacggg cggggcggtg
     1381 ggttgccgcg gcttcggctg tgacggcttc gacggtctcg acggctgact cggtggcgcc
     1441 gcttgatagt ggtgatgttg gacagtgcaa gtggccagcg acaggaagag gagcggggga
     1501 acgatcttca tgatgagtca gggatcgaga cggttacgct ggcgatggtg cctcagatga
     1561 tggcagcctc ggcgcgaaga agttgcgcgt gcaagcgctt tcatcgattg cctcaatctt
     1621 ttacggcggg agtagcgaag gacgatgtac gaccaccgtt accagcgctg ccgcggacgt
     1681 gctctcggcg cgacgtgcgc tcagccacca accatccgct tgaacacagc ggctgcgcct
     1741 ctcggggggc cctttcctgg gttgactaac ccgggcacgt ggaggccctt caagaaggtc
     1801 gtatgagcga tgttcttcaa aaacttgttg cgctggaggg ccatgccgat gagcatgtgc
     1861 tccaggaacc tgacctcgtt cggtggcgcc gaagtagcga agcgtccact cttcgtcatt
     1921 cgcgcaacca agtagagcag tggggtgccg cgctttttat cagagatggc gtcgtcgtaa
     1981 tgacggagct tgtgaagctc gaagcattcg cccttgaagc tctgcttgca ggtcttgcct
     2041 acataccacg gaagaatgcc acgaccggcg cgaatgccga atacataaca tccgcgcgcg
     2101 ctggagaggc cgggatgtgc ttcgtcgagc tccttccaga aggcttggcg atcgcgagta
     2161 tcggcgaccg cgggcttgcc tggtttgcgt ggaatctcga agccgccgaa tatttcgtag
     2221 ttcatgggtt gttgtcgtcg ctgccgagag ggagctgggt ggtggacttg caggccgttt
     2281 tggagtcttt tttctgccga ggcgtcgggg gcggtcgggg tgggtttgcc aggacttttg
     2341 ttgcgggggc cctggcgttc ttcttcggcg gcgcgctcgg cgttgagggc gaagaggcgg
     2401 tcgaggacgg cgtcctcaaa ggcctcgtag gtgaggcgct cggccggggt gcgggggggg
     2461 tgtaggacgg gacctcgata tcgccccagc cgttggcgtg gaggacggcg cggtcgagtt
     2521 cgatgggcag ggctcggagg gcgaagaccg cggggtcgtc gcagtccggg tccttcgggg
     2581 cgttgtaggt ctttgttaaa ccctgctggg tgtcgagcat gtacttcgca cgggcggtgc
     2641 agaggcgctc gccgatggac tcgacagatg tctttgagct cctcgcgctt gagcgcgtcg
     2701 agcagcttgc cgaagctggc gcgcttggaa tgcgcgagcg actcgatgat gtcggccttg
     2761 gcagcggcgc gtggcagacc ctgttcgaac aggtcggcga gctcgagcag gcgcacgcgt
     2821 gtaagcgtgg tgagggccga tcgcttgctg ggctgcgcca tgggatccgc gggccgggat
     2881 gttagcagcg cgagcgcatc ctgactatca ccccgctcgc ttgaacgccc gaagcacgct
     2941 ccgcggtcag ccgagtacgc gctcgacgaa ccagtacagc ccggcgatca cgatcgccgc
     3001 ggagatccag cgtggcccgc ggacccgctg ataggcgggc gacttgccga gcagggccag
     3061 caggggcagc acgagcgcga gcaccagcag ctgtccggcc tcgacgccga ggttgaacag
     3121 cagcagggcc gagacgatcg cgtcgtccgg cacgccgatc tcggcgagca cgccggcgaa
     3181 gccgaagccg tgcacgaagc cgaacggcag ggtgatccgc cagcggccgc cggcgtcggc
     3241 ttttaaaaaa ttctcgatcc cgacgtaggc gatcgacagc gcgatcgcgg gctcgacgaa
     3301 gccggggtcg ggggtccaga tgccgagggt cgccagcgcc aggctcagcg agtgcccgat
     3361 cgtgaacgcg gtgatcaccg cgatcagcga gcgcagccgg ccgccgatga ccaccaggcc
     3421 gagcaggaac accaggtgat cgatgccgat caggatgtgc tcgacgccga tcaagaaata
     3481 cgcgctgacc ggtggacgtg acggcgccgt cggctcgccg agctcgacgc tcgccgtcgc
     3541 ggaccggctg tgcaataccg gctcggcgag ccgcacgccc tccggggccg cgtcggtcgc
     3601 gggccgccgg acctccgcga ggtggcggtg gccggacgcc agcgtggcca gcagcggcat
     3661 gttcagctcc agccggcgcg gcgcggcagg gcaggcgtag cgcaggtgca tgcgcgcgcc
     3721 gtcaccctcg gtcagcgccg cgtcgacgac ggcgccggag cattcctggc cgtcgcccgc
     3781 gacctgcacg ccggtgacca cgacctgcaa ggcacctgtc ccggcggcca gctcgggccc
     3841 ggacagggtg ccatcgccgt cggtgtcgag cgcgggcgcg atgccgcgca gctcgtcgcg
     3901 ggcgaaggtc agctcggcgg cgaccacctc cccctcgacg acgtagacgc cgcgcgagag
     3961 gccgacctcg tgggcgagcg ccggccgcgg agtgaacacg acacatgtcc cgagcaccat
     4021 ggcccaaggg acatgtcgaa gcggccatga atttgcggag gtcgacatct ctcggggcgt
     4081 cccggccggc attcgaggtc accggtataa tcgaaggccg gatcgatcgc cagggccctc
     4141 gcctggccat ggatcgccgg cggccgggcc ccgcagcagg cgcgatcgcg tgtcgccggg
     4201 agtcgactgg ggcctgcgag ggcgcgcccc gggtcgttcg aggtcctcag cgcggtcgat
     4261 ggcaggcgac ctcgaccgcg tagccgtcgg gggcgcggca gtaggtcatc gtgccgcggt
     4321 tgttggtgtc gacgtccgag atctcgaggc cggccgcgcg ggcgcgcgtg tggatcgcgt
     4381 ggacggtcgc gacgtcgtcg acgaggaagc cgaggtgaaa gccctcgggg tagacgacgg
     4441 ggatccggcg ctgcaacacg agcacgaagc cctcgccgtc gccgaggatc gcgatcgcgg
     4501 gcgagcgcgg gttggactgc agctcgaggc cgaagatggt ggtgaagaag gcgacggtct
     4561 tctggacgtc gctgacttga agatcgaggt ggttgagtcg catcgggagc cttcctgggc
     4621 gcatggcgcc gccgaggccg tgggaactca cccgtattcg ggggttcatg cgctcccgca
     4681 ggtgcgcgct cccgcaggtg cgggaatgac cggggctcac cgaaccggtc cacctttttc
     4741 ggcgccgcga ctctatcgcg gtccgccgcg ccccgcaagc ggccggtggg gcgccttttt
     4801 cacacctcct ggcggcgcac ggaccagatg aagccgtcgt accagaagtg catcagcgag
     4861 acgacgaggg tgacgctgag ggcgaagcgt tgctcgctcg agacgaactc ggcccacacg
     4921 ccgtaggccc cggcggcggc gagcatgcag gcgagggccg cgaggccgcc ccagcgcacg
     4981 ccgtcgaggc gcatgtgtcg gcgcaggttg tcgcgctcgc tccaccacac caggccgaag
     5041 tattgccagg cgtggaagaa gttcatgatg aagaacgcct ccccgaagct gttgaacccc
     5101 caggtgtaga tcgagcacag cccggtgctg gccaggaggg cgaccttttg cggggatacg
     5161 tgataaccgc gacgatgcag tcgcaggtat ccgagcgcgt agatcagcag ataaagccca
     5221 ccgccgatca gcaggacggc ggcgagcgtc ggttgatggg tggcgacgtg ggtggggacc
     5281 tgatcgatcg ccagcgcgcc gacctcctcg agcgcgccga gcggccgcag gtggtcgagc
     5341 agcgtcgcgc cggcgaggac cgggcccatg tagagcagca ggttgagccc gaggtcgagg
     5401 cggcgtccga gcgcgggcgg gttgccgcga cgcgcatcgt agatccggcc gagcccgaag
     5461 gtctggaggc tcgagtggac gacgtcccac catacggtcg cgaccacggc gatcgcgagg
     5521 atccagtccg aggcgcacag caaggcgagc aggaccagcg gcaccgcgat gaagcgcgcc
     5581 gggtgacgcg cgaagacccg ccggttgagg tggctgcgca cggcgaccaa cgcgaggtgc
     5641 gccatgatga agctgccgat gaagatgctc gtcgccgtgc cctcgttgcc ccacagcacg
     5701 acctcctcgt ccgcccacgg cgtgcccgcg acggcgatgc cgagcgccag cgccagcagc
     5761 ggggcgccga tgaacatcac cgcgtcgtag gtcggacctg cgatgtaggg cccgcgcacg
     5821 cggagcatgc tagcgctccg cgctcgcggc gaccagtggg tcgaggggac gcgtgggggg
     5881 ccgtgtgcgc ccgcttcggg gcccgcgtac gaggtctgga ccgtcgcgcg ccgacctgcg
     5941 agactgccgc ccgagacctg cctcgaggac ccccatgaaa cacctgcccc tgagcctgtg
     6001 gaccctgtgc acgctggtcg cctgcgacgc cgcggtggtc gccagcgccc agcaagacga
     6061 gccggccgcg ccggccgcgg tcgagctcca gaaggtcgag cacagcgaga aggccgccga
     6121 ggacgcggct gccgcggtca aggtcgacgc cgacgtgaag gcgacgatca agcccgaggt
     6181 gctcgacctc gagtcgatca acttcctggt caagaagggc aaggtcaagg acgcggccgc
     6241 gctcgaga
//
LOCUS       DASWNJ010001572         9212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_664291, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001572 DASWNJ010000000
VERSION     DASWNJ010001572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 9212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9212
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664291"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VGB85_26100"
     CDS             <1..507
                     /locus_tag="VGB85_26100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137595.1"
                     /translation="CLGNCTREWLVFVTSDPATQGSLGGLVGADYECRHRATKLFLPN
                     GERYKAWISTSKAQPVDRLYHARGPYKLINGLQVAANWDALVSGKLDNPINVTELSET
                     SDGLVFTGTLPSGERAPDSTHCDDWTTGAGDKFAWFGASTESDSNWTYAVKTDCGAGA
                     NLICFEQP"
     gene            1187..1966
                     /locus_tag="VGB85_26105"
     CDS             1187..1966
                     /locus_tag="VGB85_26105"
                     /inference="COORDINATES: protein motif:HMM:NF018604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HEY0137596.1"
                     /translation="MFAFAAGSVNASALLACQTYVTHVTGTATRIGMEIANLEPLLDF
                     ALVLVCFISGAMTSGLLINGRAHRNKRPLYGLPLWFSFSVVLFAAVAGHLGVLGPFGG
                     EVTAPADFVLLSMLSFAMGMQNAAVATSTGLLVRTTHLTGPSTDLGIHLVEMMFVKDE
                     ALSLARRHAALRAGKIVSFITGAAMAVPLARHGEFLAFLLPAVVMFTGIMLSFLPGDV
                     AGEPPLDSTKEGGELVDMDTLPVPSPGSIGDPAVAPRARPL"
     gene            2049..3524
                     /locus_tag="VGB85_26110"
     CDS             2049..3524
                     /locus_tag="VGB85_26110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137597.1"
                     /translation="MNLERRMCGAALVLVACGSGAESSDTDGSTTGSTGPATGDTGTP
                     TTGSTTATTDDKGVVCPTCAGTCDLRAADGGNPAWALLCGSAVDEAQARVAATGSGSL
                     TVVFELGHAADKQVSLALGQTFVEAGQGDAIVARLGAGGALEWSQAWSGPGFARPQAA
                     AGSAAGRFAAFIGASDVTTIGAEVVTCPGECGVIAVLGPDGVLAWQRSYQGANYAFGL
                     AIGPSGEVAVAGAYAGATDFGAGPVSSSGSEPFVTVHEVDGTLRFARTYLSAGGEAAS
                     IAFAADGSLVIGGTFSKSIALGDPALVADDNATFVARLAPTGETLWARQFDSPGAFDH
                     QIKRVVIDSAGNIGIAGTFIGRLNLGGVDLDNADPAQVEDPNYDVFVAGLDPAGTHLW
                     SARLGDEENDDRIAGLDVAEPLGLQVAWAGETSGSRLDRFDGAGVSLASVVVDVPGLH
                     SAARDVDGTMMLAGSHAGPFDLLGPWTPAGEQDLWVTRILP"
     gene            3775..5490
                     /locus_tag="VGB85_26115"
     CDS             3775..5490
                     /locus_tag="VGB85_26115"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEY0137598.1"
                     /translation="MADRPRLGIEGVLGLALGLLAPACGGGDEVAQTEATTQTQGGPG
                     STDGPGPDDGTSGEGPTPTTSGGSEDTGDEPELPAVIDVPLRRLTAFEHRNTIADLFP
                     NLEPPVYELPVDDRREGFDNNADALIPSKLHIEQYNAAAEKIADLAVQDLPALIDCTP
                     DTDPNCSVRFVTEFGARAFRRPLTSAEVEAFRVFFVGPPGDTDFAAGVETTIQLMLQA
                     PQFLYRIELHEAGGTPGSLAPLDGYQLATRLSYFLWSSMPDPALFAAATQGKLATPAQ
                     VEVEVRRMLDDPKAERAFLHFFRQWGELERVDLVSKLEDDGYDDATRSALREEFERFV
                     RDVLGHGEGSFGDLLQSPRTFVNDRLAGIYGVAGPGPDMWAEVELDPTQRAGILTQPA
                     FLAGHGHPLNPSPVKRGNFVLQNLLCTNVGSPPPIAEAMGSPKPMPGMTNRQVYEELT
                     KAEQCNACHKMINPVGFAFEHYDTMGRFRELDQGLPVDASGAINGLSYADATEFMADL
                     VADPLVQNCFSRKWMIYSLGGPQLVPDMASEVSDAFATAEFNLRELQVAIATHPRFAS
                     YEVAP"
     gene            5487..6965
                     /locus_tag="VGB85_26120"
     CDS             5487..6965
                     /locus_tag="VGB85_26120"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEY0137599.1"
                     /translation="MNIFNQSRPRFDRRRFLQAMGLGTGSLFLPSLLRGAQADAEGPP
                     PRILFFFAEHGTFYPNWRMRRPDSPQSEQAEYEFDLKDLPEDQFSEILRPLYPYRDEM
                     IVLDGLALLSAMADPYGDGHAKGWCASLTGQFARETYDGVKSLASVASIDQKIKEHLR
                     AQDSALTDLTSLEYGIANWGGTFHQLSYGTDTQGTAVKVPLEVEPKKALDALFGAKDD
                     MNDPVRAARLGVVEQVRAQYEQMAPKLSGADRAKLESHRDLISDLHKRIETLQNLECT
                     QPEIMDLPYAEWTGERFAYKRDSFVELVAAAFSCQVSRVATIFSWIPPMDMIGGTGDY
                     HHDYAHQSGSDAPAEKQQVVTNAERVLAETVAKFAERLKQIPEGDGTVYDNTIIVWVS
                     ELANGGHGHNQWPAVLLGGGAKKFKGGRYLRFPETTPHPLTKDWNPGGFVGQPHQPLL
                     TSLCQAMGMEVDSTGLKSIPCLAPDSTTVEVGLTGPMERLYG"
     gene            6958..8130
                     /locus_tag="VGB85_26125"
     CDS             6958..8130
                     /locus_tag="VGB85_26125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137600.1"
                     /translation="MDRRALALGLLLVACGDDGSDTEATQATGTGSTGAVVTAGTPTT
                     GATDTGATDTGEPPGSTGDATSDASDTGDTGSNTSTGEPVGPMGHFLAIGDGGRRVRS
                     SDGVVWDGLVGSGLLDTDDELAAPDALRALAVGDGYIVAVGGGGNFWKGNAMVMRSTD
                     GGVTWQEDLLKDKPDFSPHKLYGAAASGQVVVAAGMRGKAIRSEDGGLDWTDISFEDQ
                     GSRLLGVAAQGDTFVVVGWTDNGESPDTSVVLTSPDLGLTWGAPDHSFERLDTIAAGN
                     GTFVAIGGLKCLRSQDGVAWTDCGPISAGFKGVAFAGDEFVLATAEGLATSLDGVAWT
                     TPTLPPVGSPAQLARGNGRYVGVRWTDRGWATDLEDWSFATWATEPLRSIVFIPAP"
     gene            8225..>9212
                     /locus_tag="VGB85_26130"
     CDS             8225..>9212
                     /locus_tag="VGB85_26130"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEY0137601.1"
                     /translation="MSTVLLIIVGLVIAASEGVMIGVLAPLTFWLLLHARQQRQELAA
                     LKLAVADLQEKQALAPAMPVVSVTPAVPVAGLPIYGVQPMAHELVERASMAPVIAPTE
                     HVVAATPEPDAPVPVPVRGQTEPSPASPAEPPPPPPPVAKFVPRPVYDPFAPVREWLV
                     GGNTIVKLGVAILFIGLAFLARYTADQVAVEVWLAAIAAVAVALLVLGWRLRLRRPGY
                     AQVLQGGAVAILYLTLFAAFRIYELLPVLPVFVMMVAVAALSAALAVLQDARALALLG
                     ALGGFATPLLVSSGSGNHIALFSYYLVLDLGIAGVAWFRTWRGLNLVGFLGTF"
BASE COUNT         1310 a         3245 c         3265 g         1392 t
ORIGIN      
        1 tgcctcggca actgcacccg cgagtggctc gtcttcgtca ccagcgaccc tgctacccag
       61 ggcagcctcg gcgggctcgt cggtgcggac tacgagtgcc ggcaccgcgc gaccaagctg
      121 ttcctgccca acggcgagcg ctacaaggcg tggatcagca ccagcaaggc ccagcccgtc
      181 gaccgcctct accacgctcg cgggccctac aagttgatca acggcctgca ggtcgcggcg
      241 aactgggacg ccctcgtcag cggcaagctc gacaacccga tcaacgtcac cgagctcagc
      301 gagacctccg atggcttggt gttcaccggc acgctgccca gcggcgagcg agccccggac
      361 tcgacccact gcgacgactg gaccaccggc gccggcgaca agttcgcgtg gttcggcgcc
      421 agcacggaga gcgacagcaa ctggacctac gccgtcaaga ccgactgcgg ggcgggtgcc
      481 aacctgatct gcttcgagca gccgtgacgc gggtccaccc catgcgctca cgttccggtg
      541 tccgcaccgc ggcgtctcga gcatcctggc ctgccgagca cctgccccgc cggtgcttcg
      601 atgtgcatcc cgtccaccaa taatttcacg caccgggcca tctcttcgga tcacgcgagt
      661 aggtgctttg atccttcggc gcgaccggtg tgaccttgat gtgcgctgct gtttgccgcc
      721 ttgcgatgtc acgatccggg cgtcgtatac ccgggtcgca ggagtgcctc tcgctccatg
      781 cgcgggcgct ggtagctcga agcgctggcg ccgggtagcg tgggcccggc gaggtcgagc
      841 ctcacgcccg cgagcgcacg gagcggcagt ccctcggaga cgggggccgt gtcggtcggg
      901 cgctcctcgc ggcggatggt gactcgtcga cggtgaagtc gtggtgaccg ctctgcagga
      961 cgtcaccggc acgcgagatt cgcactgtgc ccgcgatggc gatcgtcgcg gcgtcgattg
     1021 cccaccaggt ctttgattgg gccggggatc ggccctggcg ctggtcgtgc agtatatcct
     1081 gctccgtcga gcgcctcgac gagtcttcca tttcgcgctc tttgccatgc ttacgctcta
     1141 caagcccgag tccatctttt cggcccgcca cgtccccagc tggctcatgt tcgcctttgc
     1201 ggccggctcc gtgaacgcct cggcgctgct cgcatgtcag acatatgtga cgcacgttac
     1261 cggaaccgcg acgcgtattg gcatggagat cgcaaacctc gagccgctcc tcgacttcgc
     1321 gctcgtgctc gtgtgcttca tcagcggggc gatgacgtcc ggcctgctga tcaacggtcg
     1381 cgcgcatcgc aacaagcgcc cgctgtatgg attgccgctg tggttctcgt tctcggtggt
     1441 gctgttcgcg gcggtcgccg gccacctcgg ggtgctcggt cctttcgggg gcgaggtgac
     1501 cgcgcccgcc gacttcgtcc tgctgtcgat gctcagcttc gccatgggca tgcaaaacgc
     1561 ggcggtcgcc acgagcaccg gcttgctcgt gcgcacgacc cacctcaccg gcccgtccac
     1621 cgacctcggg atccacctcg tcgagatgat gttcgtcaag gacgaggcgc tctcgctcgc
     1681 caggcgtcac gcggcgctgc gcgccggcaa gatcgtgtcc ttcatcaccg gcgccgcgat
     1741 ggcggtcccg ctggcccggc acggcgagtt cctggcgttc ctcctgcccg ccgtggtgat
     1801 gttcacgggg atcatgctca gctttttgcc cggggacgtc gctggcgagc cgccgctgga
     1861 ctcgacgaag gagggtggcg agctcgtcga tatggatacc ctcccggtgc cctcgccggg
     1921 cagcatcggc gaccctgcgg tggcacccag ggccaggccg ctctgaccgc gccgcgtcga
     1981 cgtgcgagat cgctccggcg cggtgagcag gcgcgacggg gcgtcgtcgg ccgcgttatc
     2041 gtgccgcggt gaaccttgaa cgacggatgt gtggcgcggc gctggtgctc gtcgcgtgtg
     2101 gatccggggc ggagagcagc gacaccgacg gctcgaccac gggcagcacg gggccggcga
     2161 ccggcgacac cgggacgccg acgaccgggt cgacgacggc gacgaccgac gacaagggcg
     2221 tcgtgtgccc gacctgcgcc ggcacctgtg acctgcgcgc ggcggacggg ggcaacccgg
     2281 cgtgggcgct gctgtgcggc agcgcggtcg acgaggcgca ggcgagggtg gcggcgaccg
     2341 gctcgggctc gctgacggtc gtcttcgagc tcggccacgc cgccgacaag caggtctcgc
     2401 tcgcgctcgg gcagaccttt gtggaggcgg gccagggcga cgcgatcgtc gcgcggctgg
     2461 gggccggcgg ggcgctcgag tggagtcagg cgtggagcgg ccccggcttt gcgcgcccgc
     2521 aggccgcggc aggcagcgcg gccggccgct tcgccgcgtt catcggcgcc tcggacgtca
     2581 cgacgatcgg cgccgaggtc gtcacctgcc cgggcgagtg tggggtcatc gcggtgctcg
     2641 gacccgacgg cgtgctggcg tggcagcgct cgtatcaggg cgccaactat gccttcggtc
     2701 tggcgatcgg gcccagcggt gaggtcgcgg tcgcgggcgc ctacgcgggc gcgaccgact
     2761 tcggcgcggg gccggtgtcg agctcgggca gcgagccctt cgtcaccgtc cacgaggtcg
     2821 acggcacgct gcgcttcgcc cggacctacc tgagcgccgg cggcgaggcg gcttcgatcg
     2881 ccttcgcggc cgacggctcg ctggtgatcg gtgggacctt cagcaagtcg atcgcgctcg
     2941 gcgaccccgc gctggtggcc gacgacaacg cgaccttcgt cgcccgactg gcgccgacgg
     3001 gcgagaccct gtgggcgcgg cagttcgaca gcccgggcgc cttcgaccac cagatcaagc
     3061 gcgtggtgat cgacagcgcc gggaacatcg ggatcgccgg cacctttatc ggcaggctca
     3121 acctcggcgg cgtggacctc gacaacgccg acccggccca ggtcgaggac cccaactacg
     3181 acgtattcgt cgccggcctc gaccccgcgg gcacgcacct gtggagcgcg cgcctcggtg
     3241 acgaggagaa cgacgatagg atcgccggcc tcgacgtcgc cgagccgctc gggctgcagg
     3301 tcgcgtgggc gggcgagacc tcgggctcgc ggctcgaccg cttcgacggc gcgggcgtgt
     3361 cgctcgccag cgtcgtcgtc gacgtgcccg ggctccacag cgcggcgcgc gacgtcgacg
     3421 gcacgatgat gctcgccggc tcgcacgcgg ggccctttga tctgctcggc ccgtggaccc
     3481 ccgccggcga gcaggacctg tgggtcacgc ggatcttgcc gtagtcggcc gaggcatgcg
     3541 cgcgactgag cacccgggtg tgcatcgacg cgtggtcgca tggcctcatg ccgatccgtc
     3601 gaccgctcgt gcgccggggc gtgttgaccc gcgcggcggg gctggacagc cgctgccggc
     3661 aatagatgtc tcttggggca ggttcatcgc gcgcgccgat gcgatcgtga cagcgggggc
     3721 tggcgcccct tgtggaacgt cagccgtacg ccatacgatg gtggtgaggt ggtgatggcg
     3781 gatagaccca gacttgggat cgagggtgtc ctcgggctcg cgctcggact gctcgcgccg
     3841 gcgtgcggcg gcggggacga ggtcgcgcag accgaggcga cgacacagac gcagggcgga
     3901 ccgggcagca cggacggccc ggggcccgac gacggcacca gcggcgaagg tccgacgccg
     3961 acgacgtcgg gcggcagcga ggacaccggt gacgagccgg agctgccggc cgtgatcgac
     4021 gtgccgctgc gccgcctgac ggccttcgag caccgcaaca cgatcgccga cctgttcccg
     4081 aacctcgagc cgccggtgta cgagctgccg gtcgacgacc gccgcgaggg cttcgacaac
     4141 aacgccgacg cgttgatccc gagcaagctg cacatcgagc agtacaacgc ggcggccgag
     4201 aagatcgccg acctcgcggt ccaggacctg ccggcgctga tcgactgcac gccggacacc
     4261 gacccgaact gtagtgtccg cttcgtgacc gagttcggcg cgcgggcgtt ccggcgcccg
     4321 ctgacgagcg ccgaggtcga ggccttccgc gtgttcttcg tcggcccgcc cggcgacacc
     4381 gacttcgcgg ccggcgtcga gacgacgatc cagctgatgt tgcaggcgcc gcagttcctg
     4441 taccgcatcg agctgcacga ggccggcggc acgcccggct cgctggcgcc gctcgacggc
     4501 taccagctcg ccacccgcct gtcgtacttc ctgtggagct cgatgcccga cccggcgctg
     4561 ttcgccgcgg ccacgcaggg caagctcgcc acgccggcgc aggtcgaggt cgaggtccgg
     4621 cgcatgctcg acgacccgaa ggccgagcgc gcctttctcc acttcttccg ccagtggggc
     4681 gagctcgagc gcgtcgacct cgtcagcaag ctcgaggacg acggctacga cgacgcgacg
     4741 cgcagcgcgc tgcgtgagga gttcgagcgc ttcgtccgcg acgtgctcgg ccacggcgag
     4801 ggcagcttcg gcgatttgtt gcagagcccg cgcaccttcg tcaacgaccg cctcgcgggg
     4861 atctacgggg tcgcgggtcc gggcccggac atgtgggccg aggtcgagct cgacccgacg
     4921 cagcgcgcgg gcatcctgac gcagccggcg tttttggccg gtcatggtca cccgctcaac
     4981 ccctcgccgg tcaagcgcgg caacttcgtg ctgcagaacc tgctctgcac caacgtcggt
     5041 tcgccgccgc cgatcgcgga ggcgatgggg tcgcccaagc cgatgccggg catgaccaac
     5101 cgtcaggtct acgaggagct gaccaaggcc gagcagtgca acgcgtgcca caagatgatc
     5161 aacccggtcg gcttcgcgtt cgagcactac gacacgatgg gccgcttccg tgagctcgac
     5221 cagggcctgc cggtcgacgc ctcgggtgcg atcaacggcc tgagctacgc cgacgcgacc
     5281 gagttcatgg ccgacctcgt cgccgacccg ctggtgcaga actgcttcag ccgcaagtgg
     5341 atgatctaca gcctcggcgg gccgcagctg gtgcccgaca tggccagcga ggtctccgac
     5401 gcgtttgcga cggccgagtt caacctgcgc gagctgcagg tggcgatcgc cacccacccg
     5461 cgcttcgcca gctacgaggt ggcgccatga atatcttcaa ccagtcccgc ccccgcttcg
     5521 atcgtcgccg cttcctgcag gccatgggcc tcggcaccgg ctcgctgttc ttgccctcgc
     5581 tgctgcgcgg cgcgcaggcg gacgccgagg gccctccgcc gcggatcctg ttcttcttcg
     5641 ccgagcacgg gacgttttat cccaactggc gcatgcgccg gcccgactcg ccgcagtccg
     5701 agcaggccga gtacgagttc gacctcaagg acctgcccga ggaccagttc agcgagatct
     5761 tgcggccgct gtacccgtac cgcgacgaga tgatcgtgct cgacgggctc gcgctgctca
     5821 gcgcgatggc cgacccctac ggcgacggcc acgccaaggg ctggtgcgcc tcgttgaccg
     5881 gccagttcgc gcgcgagacc tacgacggcg tcaagtcgct cgcgtcggtc gcctcgatcg
     5941 atcagaagat caaggagcac ctgcgcgcgc aggacagcgc gctgaccgac ctgacctcgc
     6001 tcgagtacgg catcgccaac tggggcggga ccttccacca gctgtcctat gggacagaca
     6061 cgcagggcac ggcggtcaag gtgccgctcg aggtcgagcc caagaaggcc ctcgacgcgc
     6121 tgttcggggc caaggacgac atgaacgacc cggtccgcgc ggcgcggctc ggggtcgtcg
     6181 agcaggtgcg ggcgcagtac gagcagatgg cgccgaagct gtcgggcgcc gaccgtgcga
     6241 agctcgagag ccaccgcgac ctgatctccg acctgcacaa gcgcatcgag acgctgcaaa
     6301 acctcgagtg cacgcagccg gagatcatgg atctgccgta cgccgagtgg accggcgagc
     6361 gcttcgcata caagcgcgac agcttcgtcg agctggtcgc ggcggcgttc tcgtgccagg
     6421 tctcgcgggt cgccacgatc ttctcgtgga tcccgccgat ggacatgatc ggcggcacgg
     6481 gcgactacca ccacgactac gcgcaccagt cgggctccga cgcgccggcc gagaagcagc
     6541 aggtcgtcac caacgcggag cgcgtgctcg ccgagacggt ggcgaagttc gccgagcggc
     6601 tcaagcagat ccccgagggc gacggcacgg tgtacgacaa cacgatcatc gtgtgggtct
     6661 ccgagctcgc caacggcggc cacgggcata atcaatggcc ggcggtgctg ctcggcggcg
     6721 gggccaagaa gttcaagggc gggcggtacc tccgcttccc cgagacgacc ccgcacccgc
     6781 tcaccaagga ctggaacccc ggcggcttcg tcggccagcc gcatcaacca ctgctgacct
     6841 cgctgtgcca ggcgatgggc atggaggtcg actcgaccgg cctgaagagc atcccttgcc
     6901 tcgcgcccga cagcaccacc gtcgaggtcg gcctgaccgg accgatggag cggctgtatg
     6961 gatagacgcg cgctcgcact cggcctgcta ctggtcgcct gcggcgacga cggcagcgac
     7021 acggaggcca cgcaggcgac cggcaccggc agcaccggcg ccgtcgtgac ggccggcacg
     7081 ccgacgaccg gcgcgaccga cacgggcgcg accgacaccg gtgagcctcc gggcagcacc
     7141 ggcgacgcga cgagcgacgc gagcgacacc ggcgacaccg gctcgaacac cagcacgggc
     7201 gagccggtag ggccgatggg gcatttcctg gcgatcggcg acgggggccg gcgcgtgcgc
     7261 tcgagcgatg gcgtcgtatg ggacgggctg gtcggcagcg gcctgctcga caccgacgac
     7321 gagctggccg cgccggacgc cctgcgcgcg ctcgcggtcg gcgatggtta catcgtcgcg
     7381 gtcggcggcg gcggcaattt ttggaagggc aacgcgatgg tcatgcgcag caccgatggc
     7441 ggcgtgacct ggcaggagga cctgctcaag gacaagcccg acttctcgcc gcacaagctc
     7501 tacggcgcgg ccgcgagcgg gcaggtggtg gtggcggccg gcatgcgcgg caaggcgatc
     7561 cgcagcgagg acggcgggct cgactggacc gacatctcgt tcgaggatca gggctcgcgc
     7621 ctgctcggcg tcgcggccca gggcgacacc ttcgtggtcg tcggctggac cgacaacggt
     7681 gaatctccgg acacctcggt ggtgctcacc agcccggacc tcggcctgac ctggggcgcg
     7741 cccgaccact cgttcgagcg cctcgacacg atcgcggccg gcaacggcac gttcgtggcg
     7801 atcggcggcc tcaagtgcct gcgctcgcag gacggcgtcg cctggaccga ctgcggaccg
     7861 atctcggccg gcttcaaggg cgtcgccttc gccggcgacg agttcgtgct cgccaccgcc
     7921 gagggcctcg ccacctcgct cgacggcgtg gcctggacca caccgacgct gccgcccgtc
     7981 ggctcccccg cgcagctggc ccgcggcaac ggccgctacg tcggcgtccg ctggaccgac
     8041 cgcggctggg cgaccgacct cgaggactgg agcttcgcga cctgggcgac cgagccgctg
     8101 cgctcgatcg tgttcatccc ggccccataa atcggatgac cgggtgatcg ggtgaccgcg
     8161 ccggctttgt acaaccggcg cggtcgctga tacgctgcga tgcggccggg cggccgaacg
     8221 cgacgtgtcg actgtcctgc tgatcattgt cggtctggtg atcgcggcgt ccgagggcgt
     8281 gatgatcggt gtgctcgcgc cgctgacgtt ctggttgctg ttgcacgcgc gccagcagcg
     8341 gcaagagctc gcggcgctca agctcgcggt cgcggatctg caggagaagc aggccctcgc
     8401 gcccgcgatg ccagtcgtgt cggtgacccc ggcggtgccg gtcgcgggtc tgccgatcta
     8461 cggcgtacag ccgatggcgc acgagctcgt cgagcgtgca tcgatggcgc ccgtgatcgc
     8521 gccgacggag cacgtggtcg cggcgactcc cgagccggac gcgccggtgc cggtgccggt
     8581 gcgcgggcag accgagccgt cgccggcgag cccggccgag ccgccgccgc caccaccgcc
     8641 agtcgcgaaa ttcgtcccgc ggccggtgta cgacccgttc gcgccggtcc gcgagtggct
     8701 cgtcggcggc aacacgatcg tcaagctcgg cgtggcgatc ctgttcatcg ggctcgcgtt
     8761 cctcgctcgc tacaccgccg accaggtcgc ggtcgaggtt tggctggcgg cgatcgccgc
     8821 ggtcgcggtg gcgctgctgg tgctcggctg gcgcctgcgc ctgcgccggc ccggctacgc
     8881 gcaggtgctg cagggcggcg cggtggcgat cctctacctg acgctgttcg ccgcgttccg
     8941 catctacgag ctgctgccgg tgctgccggt gttcgtgatg atggtcgcgg tcgcggcgtt
     9001 gtccgcggcg ctcgcggtgc tgcaggacgc ccgcgcgctg gccctgctcg gcgcgctcgg
     9061 cgggtttgcg acgccgctgc tggtgtcgag cggcagcggc aaccacatcg cgctgttcag
     9121 ctactacctg gtgctcgacc tcgggatcgc cggggtcgca tggttccgga cctggcgcgg
     9181 gctcaacctc gtcggcttcc tcggcacgtt cc
//
LOCUS       DASWNJ010001573         2433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_624220, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001573 DASWNJ010000000
VERSION     DASWNJ010001573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624220"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VGB85_26135"
     CDS             <1..511
                     /locus_tag="VGB85_26135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137602.1"
                     /translation="FIGNLGSVPDTGPGGRPERGASNLSRGTPTWLILLIGLGLVGGG
                     VAYAMLSAGGDNTEATEEAPAEVAPPAPAPVAPVTPVAPVAGTPPVPGAPGAPATPVA
                     GTTPPAATPPATPATPPAAPAPAPKKTTKPKSTTPKPAPAPAPAPEPKKITPGKKPRE
                     LDELPPPPK"
     gene            complement(606..953)
                     /locus_tag="VGB85_26140"
     CDS             complement(606..953)
                     /locus_tag="VGB85_26140"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY0137603.1"
                     /translation="MTVAVERRATARIDKLFRVLVFSEEFGEQWFCARNISETGMFVE
                     MPEPLPLRSKVVVRFQIPGDDAAICAIARVQNHYYLQYAGTHGLQALSGVGLRFLRFV
                     AEIGGAVAQDRLH"
     gene            1130..2131
                     /gene="era"
                     /locus_tag="VGB85_26145"
     CDS             1130..2131
                     /gene="era"
                     /locus_tag="VGB85_26145"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEY0137604.1"
                     /translation="MSPQRPHDPPPDEPVDPAVPADFRAGQVGLCGRPNVGKSTLLNA
                     LVGAELAVATRFPQTTRERLLGVWTTDKLQAVLVDTPGIHRARSALNRFMVDEALRGA
                     RAVDLLLLLAEVPQLPDPEAAATWEPGEGAIAALEALKEVKKPITLVMTKVDRLHPPE
                     LLLPVIAKWTALHEFAAVLPVSAHTGAGLDALRAHIAAGLPLRAPYFEEDQLSDRSMR
                     WHAGERVRAALFRHLGDELPYACAVTIEAFKEQKRPPKDIIRATIHVERESQKPMVIG
                     KGGQTIKAISIDARKAISTLTGRACDLFLNVAVTANWTRDAALMQRLGLHEAIGGES"
     gene            2128..>2433
                     /locus_tag="VGB85_26150"
     CDS             2128..>2433
                     /locus_tag="VGB85_26150"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEY0137605.1"
                     /translation="MSAEDEKLLDHVEHVEDVEHVEDADFDATVDGEFAEDMAGVAIA
                     SGPTSLVAIVGRPNVGKSTLFNRLVGAKKAIVEDRPGVTRDRLYGAVNHEGHSYLVVD
                     "
BASE COUNT          389 a          882 c          837 g          325 t
ORIGIN      
        1 cttcatcggc aacctcggct cggtgcccga caccggcccg ggtggccgtc ccgagcgtgg
       61 ggcgagcaac ctgagccgcg gcaccccgac ctggctgatc ctgctgatcg gcctcgggct
      121 cgtcggcggc ggggtggcct acgcgatgct cagcgccggt ggcgacaaca ccgaagccac
      181 cgaggaggcg cccgccgagg tcgcgccccc ggctcctgcc ccggtcgcac cggtgacccc
      241 tgtcgcgccg gtcgccggca cgccgccggt tccgggtgcc ccaggtgccc cggccacgcc
      301 ggtcgccggc acaaccccgc cggccgcgac gccgccggcc acgcccgcga cgccgccggc
      361 cgcgcctgcg cccgcgccga agaagacgac caagcccaag tccacgacgc cgaagccggc
      421 gcccgcaccc gcgccggcgc ccgagccgaa gaaaatcacg cccggcaaga agccgcgcga
      481 gctcgacgag ttgccgccgc cgccgaagtg acacctgtcc cgaaggccat gtccttcggg
      541 ccagccaaaa caaagggccc cgacgatcgc tcgccggggc cctcgatggt taatctccgt
      601 cgcttttagt gcagtcggtc ctgcgcgacg gcgccgccga tctcggcgac gaagcgcagg
      661 aaccggagcc cgacgcccga cagcgcctgg agcccgtggg tgccggcata ttgcaggtag
      721 taatggttct ggacgcgggc gatcgcgcag atggcggcgt cgtcgccggg gatctggaag
      781 cgcacgacga ccttgctgcg cagcggcagg ggctcgggca tctccacgaa catgcccgtc
      841 tccgagatgt tgcgggcgca gaaccactgc tcgccgaact cctccgagaa gacgaggacg
      901 cgaaagagct tgtcgatccg ggcagtggcg cggcgttcaa cggcgacggt catggatcgc
      961 agcatcgctg tgtcgggcca tggaatcaaa tatcctacat gtatgactcg gtaggcccgg
     1021 aagaccccgg aggtcgcccg cgtgcggccg gtcatccgcc ggggcgggcg cacgcgcgat
     1081 ccgtgcttgc aggcgccggg gatttggcca ataaccccgc attcgagcga tgtcccccca
     1141 gcgaccccac gatccgcccc ccgatgaacc ggtcgatccc gccgtgcccg cggacttccg
     1201 cgccgggcag gtcggactct gtggccggcc caacgtgggc aaatccactc tcctcaatgc
     1261 gctggtgggc gccgagctgg cggtcgccac ccgcttcccg cagaccaccc gcgagcgcct
     1321 gctcggcgtg tggaccacgg acaagctgca ggcggtgctc gtcgacacgc cggggatcca
     1381 ccgcgctcgc tcggcgctca accgcttcat ggtcgacgag gccctgcgcg gagcccgggc
     1441 cgtcgacctg ttgttgttgc tcgccgaggt cccgcagctg ccggaccccg aggcggccgc
     1501 gacctgggag ccgggggagg gcgccatcgc ggcgctcgag gctctcaaag aggtcaaaaa
     1561 gccgatcacg ctggtgatga cgaaggtcga tcgcctgcac ccgcccgagc tgttgttgcc
     1621 ggtgatcgcc aagtggacgg cgctgcacga gttcgccgcg gtgctgccgg tcagcgcgca
     1681 caccggcgcc ggcctcgacg cactgcgggc gcacatcgcc gccggcctgc cgctgcgcgc
     1741 cccgtacttc gaggaggacc agctcagcga tcgcagcatg cgctggcacg cgggcgagcg
     1801 cgtgcgtgcg gcgctgttcc gccacctcgg cgacgagctg ccctacgcgt gcgccgtcac
     1861 gatcgaggcg ttcaaggagc agaagcggcc gcccaaggac atcatccgcg cgacgatcca
     1921 cgtcgagcgc gagagccaga agccgatggt gatcggcaag ggcggccaga cgatcaaggc
     1981 gatctcgatc gacgctcgca aggcgatctc gacgctgaca ggccgcgctt gcgacctgtt
     2041 cctcaacgtc gcggtcaccg cgaactggac ccgcgacgcc gccttgatgc agcgcctcgg
     2101 gctccacgag gccatcggag gtgagtcgtg agcgccgagg acgagaagct acttgatcat
     2161 gttgagcacg ttgaggatgt cgagcacgtc gaggatgccg acttcgacgc gacggtcgac
     2221 ggcgagttcg ccgaggacat ggccggcgtt gcgatcgcgt ccggaccgac cagcctggtg
     2281 gcgatcgtcg gccggcccaa cgtcggcaag tcgacgctgt tcaaccggct cgtcggcgcc
     2341 aagaaggcga tcgtcgagga ccgccccggc gtgacgcgcg accgcctgta cggcgcggtc
     2401 aaccatgagg gccacagcta tctcgtcgtc gac
//
LOCUS       DASWNJ010001574         2387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_311476, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001574 DASWNJ010000000
VERSION     DASWNJ010001574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311476"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1753)
                     /locus_tag="VGB85_26155"
     CDS             complement(<1..1753)
                     /locus_tag="VGB85_26155"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137606.1"
                     /translation="MPRPTPAPDTHARQESLQHLGWPLVCLALAEHLRTPMALARLEA
                     ELDGHDRGGGGLLGAEAYAGSIAGRSAEADAVSLRFREQDGLAAMLAVQASAGIRATA
                     PEMRASAPGGSQNLADALSRTSDLLDVAQRAGRNLTLTAAEMYAALQQLLAAGLCVDL
                     HAAVRAERGYADDPGVPAIGARVSALRPHRELATTLARSLGPEGPDGEPGVVDGASPG
                     LARARSRVRELKTQLTKVAQRLMKQPGIAEALQDTFYTQREGRVVLPVRADAFSRRGA
                     MSGIIHDASASGQTLYVEPQALIEENNALREAQMDAAAEERKVLEQLSRLVHVAADGL
                     RTNQEELIAIDAVHARLRLAERYGGVTPLLGEDVPEDRRGQFVLPGAKHPLMLLAGVD
                     VVPNDIYLGVGSALVISGPNAGGKTVALKTLGLSVLMAQAGLRLATRGPACLPSVRRI
                     VTDVGDDQSIAANLSTFSAHMRHVCAALASAEHDGAGTLVLLDEVAVGTEPEQGAALA
                     EAILLDLTAHGATVVTTTHYERLKLLAVQLPERFVNASVGFDLERLMPTFRLLLGVPG
                     PSSALTVARRLGLPERVL"
     gene            1960..>2387
                     /locus_tag="VGB85_26160"
     CDS             1960..>2387
                     /locus_tag="VGB85_26160"
                     /inference="COORDINATES: protein motif:HMM:NF014012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HEY0137607.1"
                     /translation="MPALFLVLLLGAFWALLSGQFHNNFLIAAGVLCITFIVWMSRRM
                     GLLDDEGVPARFYPRALAYLPWLTWQVVLSNWDVFKRIWSRDMPIDPRMAWIPYSTKH
                     PFVTTTYANSITLTPGTVTVVVDDTRMLIHCLAKDTEDGLL"
BASE COUNT          340 a          909 c          775 g          363 t
ORIGIN      
        1 cgagcacccg ctccggcagc ccgagccgcc gcgcgaccgt caacgcgctc gacggccccg
       61 gcacgcctaa caacaggcga aacgtcggca tcagccgctc gaggtcgaag ccgaccgatg
      121 catttacgaa ccgctccggg agctgcaccg cgagcagctt gagccgctcg tagtgggtcg
      181 tcgtcaccac cgtcgccccg tgggccgtca ggtcgagcag gatcgcctcg gcgagcgccg
      241 ccccctgctc cggctccgtg ccgaccgcga cctcgtcgag cagcaccagc gtgcccgccc
      301 cgtcatgctc cgccgaggcc agcgcggcgc acacgtgccg catgtgcgcc gagaaggtgc
      361 tcaggttggc cgcgatgctc tggtcatcac cgacatcggt gacgatcctg cgcaccgacg
      421 gcagacacgc cggcccgcgc gtcgccagcc gcagccccgc ctgggccatc agcaccgaca
      481 gcccgagggt cttgagcgcg accgtcttgc cgcccgcgtt cggcccgctg atcaccagcg
      541 ccgaaccgac gccgaggtag atgtcgttgg gcaccacgtc cacccccgcg agcagcatca
      601 gcgggtgctt cgcgcccggc agcacgaact gcccgcgcct gtcctcgggg acatcctcgc
      661 cgagcagcgg cgtcaccccg ccgtagcgct ccgccaggcg cagccgcgcg tgcaccgcgt
      721 cgatcgcgat cagctcctcc tggttcgtgc gcagcccgtc cgccgcgacg tgcaccagcc
      781 tgctcagctg ctcgagcacc ttgcgctcct ctgccgccgc gtccatctgc gcctcgcgca
      841 aggcgttgtt ctcttcaatc aaggcctgtg gttcgacgta cagcgtctgc ccgctcgcgc
      901 tcgcgtcgtg gatgatcccc gacatcgccc cgcgccgcga gaacgcgtcc gcccggaccg
      961 gcagcaccac ccggccctcg cgttgcgtat aaaacgtgtc ctgcagcgcc tcggcgatgc
     1021 ccggctgctt catcagccgc tgcgccacct tggtcagctg cgtcttgagc tcgcggacgc
     1081 gcgaacgcgc gcgggccagc cccgggctcg cgccatcgac cacccccggc tcaccatcgg
     1141 gcccctcggg ccccagggat cgtgccagtg tggtcgccag ctcgcggtgc ggccgtaacg
     1201 cggagacccg cgccccgatc gccggcaccc ccggatcgtc cgcgtaacct cgttcggcgc
     1261 gcaccgccgc gtggaggtcg acgcacagcc cggccgcgag cagctgttgc agcgccgcgt
     1321 acatctccgc agccgtcagc gtcagattgc gcccggcccg ctgcgcgacg tccaggagat
     1381 cgctcgtccg actcagtgca tctgcgaggt tctgcgagcc gccgggggcc gacgcccgca
     1441 tctccggcgc cgtcgcacga atacccgccg acgcctgcac cgcgagcatc gccgcgagcc
     1501 cgtcctgctc gcgaaagcgc agcgaaacag cgtccgcctc ggcgctgcgc cccgcgatgc
     1561 tccccgcgta ggcctcggcg ccgagcagcc cgccccctcc gcggtcgtgc ccgtcgagct
     1621 cggcctcgag acgcgccagc gccatcggcg tgcgcaggtg ctccgcgagc gcgaggcaca
     1681 cgagcggcca gccgaggtgt tgcagggatt cttgccgggc gtgggtgtcc ggggcgggcg
     1741 tcgggcgagg catgatcaaa ggaggtatcg gcgtcgaggc gagtggcgag gcgagtgaag
     1801 cgacgtgtag gggcaggcgg gatgcgtgca gcaacatgac gcatgtcgtg tcgccgtgcc
     1861 cccgtctgct tgcagtagca acgtcgcgcc tgggcctgcc aggcgcgatc ttttctatag
     1921 gcacgtctgg cgcaaacgga tagcgtaaag ctgccgatga tgcccgcctt gtttttggtc
     1981 ctgctgctgg gtgcgttttg ggcgctgctc agcgggcagt ttcacaacaa cttcctcatc
     2041 gccgccggcg tgctttgcat caccttcatc gtctggatga gccggcgcat gggcctcctg
     2101 gacgacgagg gcgtgccggc gcgcttctat ccccgcgcgc tggcgtacct gccgtggctc
     2161 acctggcagg tggtgctctc gaactgggac gtgttcaagc ggatctggtc gagggacatg
     2221 ccgatcgacc cgcgcatggc gtggatccct tacagcacga agcacccctt cgtcaccacg
     2281 acctacgcca actcgatcac gctgaccccc ggcaccgtga ccgtcgtcgt cgacgacacg
     2341 cgcatgctga tccactgcct ggcgaaggac accgaggacg ggctgct
//
LOCUS       DASWNJ010001575         1718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_240146, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001575 DASWNJ010000000
VERSION     DASWNJ010001575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240146"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1198)
                     /gene="brxD"
                     /locus_tag="VGB85_26165"
     CDS             complement(<1..1198)
                     /gene="brxD"
                     /locus_tag="VGB85_26165"
                     /inference="COORDINATES: protein motif:HMM:NF022376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding protein BrxD"
                     /protein_id="HEY0137608.1"
                     /translation="MSNIGEAATIVNALRSGLVPAQGLEHFATGLDELVEAVNQELDF
                     VASGKGMSKWVRGEYGTGKTFAARYLCSRARARNFATSEVQISINDTPLHHLETVYRR
                     LIEHVETAADGPNAFQAIVDGWLYKIGDEVMRLRGITEEDPGFADATEQRLEDKLAEM
                     SRRNPAFAQVLRAYHRATHEGDFATAQGLLAWLAGQPHTDRSVLRSAGTKGKVDGQAS
                     LTFLAGLLQLLRQSGYGGLVVVLDEVETIQRMNSQTREKSLNALRQLMDMLAKEELPG
                     LYLVITGTRDFFEGYKGLKGLAPLYQRVHTSFGDDPTLDNLRATQVRLLPFTQDRLLT
                     VGRRVRDLYPAKNVSRIAARVDDQFLGALVTQITSGFGGKVGLAPRLFLRELIDVLDR
                     VDLHERY"
     gene            complement(1198..>1718)
                     /locus_tag="VGB85_26170"
     CDS             complement(1198..>1718)
                     /locus_tag="VGB85_26170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137609.1"
                     /translation="SGVYLGSPQVGFHGGCSLEEMVVPLVWLERDGLPADEPSWWYGQ
                     GAVTPPIPPSRPFEPPLAAPLPSDELQPPQRPQLTLFNPGDRADTLPLPPELLKRLSN
                     DERSVLVLLRENGTARATELSERLKKNPGRLNGLMVTLKRTLHAEGSALFSDEKLPSG
                     ETMYRYIGKESR"
BASE COUNT          252 a          534 c          646 g          286 t
ORIGIN      
        1 cgtagcgctc gtgcaggtcg acgcggtcga ggacgtcgat gagctcgcgg aggaacagac
       61 gcggggcgag gccgaccttg ccgccgaagc ccgaggtgat ctgggtgacg agggcgccga
      121 ggaattgatc gtcgacgcgc gcggcgatcc ggctgacgtt cttcgccggg tacaggtcgc
      181 gcacgcggcg gccgacggtg agcaggcggt cctgcgtgaa cggcagcagc cgcacctgag
      241 tcgcgcgaag gttgtcgaga gtcgggtcat cgccgaagct cgtgtgcacg cgctggtaca
      301 gcggggcgag gcccttgagg cccttgtagc cctcgaagaa gtcgcgggtg ccggtgatga
      361 cgagatagag gcctggtagc tcctccttcg ccagcatatc catgagctgg cgcagggcgt
      421 tgagcgactt ctcgcgggtc tgcgagttca tgcgctggat ggtctcgacc tcgtcgagca
      481 cgacgaccag gccgccgtac ccagactggc gcaggagctg gagcagcccg gcgaggaagg
      541 tcagtgacgc ctggccgtcg actttgccct tggtgccggc cgagcgcagg acggagcggt
      601 cggtgtgggg ctgtccggcg agccaggcga gcaggccctg cgcggtggcg aagtcaccct
      661 cgtgggtcgc gcggtgatag gcgcgcagga cctgggcgaa ggcggggttg cgcctggaca
      721 tctcggcgag cttgtcttcg aggcgctgct cggtcgcgtc ggcgaacccg gggtcctcct
      781 ccgtgatgcc gcgcaggcgc atgacctcgt cgccgatctt gtataaccat ccgtcaacga
      841 tcgcctggaa cgcgttgggg ccgtcggcgg cggtctccac gtgctcgatg aggcggcgat
      901 agaccgtctc caggtgatgc agcggcgtgt cgttgatcga gatctggacc tcggaggtcg
      961 caaagttgcg agcccgggcc cgggagcaca ggtagcgcgc agcgaaggtc ttgccggtcc
     1021 cgtattcgcc gcggacccac ttcgacatgc cctttccgct ggcgacaaag tcgagctctt
     1081 ggttgaccgc ctcgacgagc tcatcgagcc cggtggcgaa gtgttcgagg ccctgcgccg
     1141 gcaccaggcc gctgcgcagc gcgttgacga tcgtcgcggc ttctccgatg ttggacatca
     1201 gcggctctcc ttgccgatat agcgatacat ggtctcaccg ctcggcagct tctcgtcgct
     1261 gaacagcgcg ctgccctcgg cgtgcagcgt gcgcttgagg gtgaccatga ggccgttgag
     1321 gcgcccgggg ttcttcttca ggcgctcgga gagctcggtc gcgcgggcgg tgccgttctc
     1381 tcgcagtagg accagcacgg agcgctcgtc gttgctgagc cgcttgagca gctcgggggg
     1441 cagcggcagg gtatccgcgc gatcgccagg gttgaagagc gtcagctgcg gtcgctgcgg
     1501 cggctggagc tcgtccgagg gcaacggcgc cgcgagtggc ggctcgaagg ggcgcgacgg
     1561 ggggatcggc ggggtgacag cgccctggcc gtaccaccag gagggctcgt ccgcgggcag
     1621 gccgtcgcgc tcgagccaga ccagcgggac gaccatctcc tcgaggctgc atccgccgtg
     1681 gaacccgacc tgtggtgagc cgaggtacac gccgctct
//
LOCUS       DASWNJ010001576         2454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_167876, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001576 DASWNJ010000000
VERSION     DASWNJ010001576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167876"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            471..692
                     /locus_tag="VGB85_26175"
     CDS             471..692
                     /locus_tag="VGB85_26175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137610.1"
                     /translation="MRRQVRRLSRDVGPTGLAVDAEGNIFLVGLGFHNEQRRSYLARF
                     HPDGTLYWEKPGDVTWSKSSCWRPLPSSE"
     gene            1259..>2454
                     /locus_tag="VGB85_26180"
     CDS             1259..>2454
                     /locus_tag="VGB85_26180"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY0137611.1"
                     /translation="MATKRHPLQAWLVAASVVLGGCLSSSVAADLADVRARTRVPVLA
                     DVADLTVDPDTADDARGLLEAPLDADTAVRVALLNNRALRAQLRELGVARGRLIQARQ
                     VANPRFEVESLPERNTSFEFSVEYDLTSLVLAPLRARAAAADLEAARLEVAGDVVRLG
                     YQVRSAFYTLQGAAQRLAIAQQALDAFAASRDAAAALLEAGSIPALDASSQIAAFEKA
                     RVTVARLELEVAARREGLQRLLGLHGQDTQWAIQGELPPVPEQFPLADGPEARVVQAS
                     LELAAMRSRLEAIARRTKLSRTQGWLPEIDVDMHALAGNPETGAVKGPVRVGAGVSLS
                     VPLFNQQRGTTRAHEAEFDALMERYHGLAIDLRSAARESLNRATSAYSRARQYQRVIL
                     PAQRRVV"
BASE COUNT          361 a          883 c          803 g          407 t
ORIGIN      
        1 cgtgccgagg atagcacagg cagtgcccac gatcacccca atctagatcg agcgttcttg
       61 gtcaattgaa cgatgttcgg tgcacccgac cgggcgcgga gtatccactc ggttatttgg
      121 catcccttgt tgacacctgt cgttgaaatc ttacgctcga gcgtggagga ccacagacaa
      181 tgcagatcag aatggctcac atccgtgagc aaggtcagga cctggcaatt ttcgatgcga
      241 agccgacctc tgacagtgac agttctcggg cgagacttct ctctcaactg accagcgctg
      301 ccgaagccgc gggcctgaat gttgataagg cagctctggt tttcacgcgc aacggtaggc
      361 tcgcgtattt cggtccgcct gacctcgtag acttcctgaa acgaagacgg ctgccttcgt
      421 ggaacaggac actaacaatt tcctaagtcc acgcaaggtt gcgcgccgac ttgcgccgcc
      481 aagtcaggag gctcagccgc gatgtcggac ccaccgggct cgcggtcgac gccgagggca
      541 acatcttcct cgtcggcctg ggcttccaca acgagcagcg gcgcagctat ctcgcccgct
      601 tccatcccga cggcacgctg tattgggaga agccaggcga cgtgacctgg tcgaagtcat
      661 catgttggcg accattgcca tcgtcggagt gatcatgccg acggtcgtgc tcgcgtcgac
      721 ctcgccggtg gttggttggg ccgcccggtg ctgtccgtgc tctcaagacc gctggcttcc
      781 gtcgtgccgc ctgcgctcgt gacctcaccg gttccagtaa cgcagccgca ggtccccagc
      841 agcagggcga ggagagcgtt tggcgagttc gtcatgctgc ccttgtcgcg gcatcgcagg
      901 gcgtcgtcaa gcgcgtcgtg ggcgtcggct tccattgctc gcgcggggtg taatgccggc
      961 tccgggggtg cgtcagcccg gtgaacgagg agaatactga tgcgctcatg gttgaatggc
     1021 acgcgcccgc tcgcgctggc cctgatagtt ctcgcgctgc cgggctgtgc gagccgcacg
     1081 gtcccgacgc agtttccgac gtcctccgcg gcgtcgcctg ccgctccgca gcccgccgcg
     1141 gccgtggtga cgggctcgct caccgaggag ccgccgctgc cgggacagcc cgcgacgcag
     1201 tggccgggtc ttcaggtcgg ccagggcgag ccccaagcgt ccgcgcacgg aggccatcat
     1261 ggccactaag cgacacccgc ttcaggcgtg gctcgtggct gcgtccgtcg tgctcggcgg
     1321 ctgcctctca tcctcggtcg ccgccgacct cgccgacgtg cgcgcccgga ctcgcgttcc
     1381 cgtgctcgcc gatgtcgccg acctcacggt cgatccagac accgccgacg acgctcgggg
     1441 tttgctcgag gcgccgctcg acgccgacac cgccgtccgc gtcgccctgc tcaacaaccg
     1501 cgctctacgg gcccagctcc gcgagcttgg cgtcgcccgc ggccgactga tccaggcgcg
     1561 tcaggtcgcc aaccctcgct tcgaggtcga gtcgctgccc gagcgcaaca cgtccttcga
     1621 gttcagcgtc gagtacgacc tgacctcgct cgtgctcgcg ccgctgcgag ccagggccgc
     1681 tgccgccgac ctcgaggccg cgcggctcga ggtcgccggg gacgtcgtcc gtctcgggta
     1741 tcaggtccgc agcgccttct acacgctgca gggcgccgcg cagcggctgg cgatcgccca
     1801 gcaggcgctg gacgccttcg cggcgagccg cgacgcggcg gcggccctgc tggaggccgg
     1861 cagcatcccg gcgctcgacg cctcgtcgca gatcgccgcc ttcgagaagg cgcgcgttac
     1921 cgtcgcccgg ctcgagctcg aggtcgccgc ccggcgcgag ggcctgcagc gcctcctcgg
     1981 acttcacggc caggacaccc agtgggcgat ccagggcgag ctcccgccgg tgcccgagca
     2041 gttcccgctc gccgacggac ccgaggcccg ggtcgtgcag gccagcctcg agctcgccgc
     2101 gatgcgcagc cgactcgagg ccatcgccag gcgcaccaag ctctcgcgca cgcagggctg
     2161 gctgccggag atcgacgtcg acatgcacgc gctggctgga aatcccgaga ccggcgccgt
     2221 caagggcccc gtccgcgtcg gcgctggcgt cagtctcagc gtcccgctgt tcaaccagca
     2281 gcgaggcacc acccgcgcgc acgaggccga gttcgacgcg ctgatggagc gctaccacgg
     2341 gctggcgatc gacctccgct ctgcggcccg cgagtccctc aaccgcgcga cctcggccta
     2401 ctcccgcgcg cggcagtacc agcgggtgat cttgccggcg cagcgtcgcg tcgt
//
LOCUS       DASWNJ010001577         3012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_167900, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001577 DASWNJ010000000
VERSION     DASWNJ010001577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167900"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1718)
                     /locus_tag="VGB85_26185"
     CDS             complement(<1..1718)
                     /locus_tag="VGB85_26185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137612.1"
                     /translation="MKCPTLAVLAAIWILPSAQAFAQVPGPQGTNSGQTTSSPSTAPD
                     VASTTPASIPVGTPTATAPSPTPNQTPPIDPAAPVTEGPSETEKHAADLRRLQSELAR
                     MQVRLEATEQRLSEMGVDRNREEIARNIQGPPVGFNAWTSGNAMATRVTFAIADDNIL
                     AGAQDRSPGAGFKLAADRLFYDAIEQRKRGFETETQLVVYKRVPTYFKRMDAEAAFVV
                     ELQHYTNEKTYKTETQIRDDGSYIKLNWYTKRSDYNGDQVSLTMFPFDSQRMLLGYTY
                     NITWGGERTFPNNAPGGDAARSNTMVPGMRLRYDFKVGTGKDGYVWGGAKTARLLNEQ
                     INEPQTYYGLLGGWGLSFTRWLTWEVNGGYFQRGAFPPQGTKYPGIGGKTAQAFGGST
                     RLTFRKGMPIGTSIDFQLFRYSPDATYLLTQTQTYDNRFAASGAFEFTTVGQTLLDWE
                     KPDTTVIRPAMAGAFNGRLRYKKTRVFFTALFRNLDYVVLDIPGVAPYRAFPQGAIVK
                     PEWFVVAGVDYYLQRTRLTPGFVFAYKNPASYTSNNTTLVYREFNDVETLPNGYAAYD
                     ILSSKLT"
     gene            complement(1778..>3012)
                     /locus_tag="VGB85_26190"
     CDS             complement(1778..>3012)
                     /locus_tag="VGB85_26190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137613.1"
                     /translation="DLKDLVVTSPIYYDKKSNGNFFVSEAGGGPFSGILVYAYADVIA
                     ELDAEGSLPAVGDKIDLRATYTEFYDYSELTLSAGVDLKITGAGTVPAPTTVTAADVA
                     TGGPKAEDFEGCLIQIAGAKVTAPVADFGEFQVDGVLQVDDLFFIPNPGPKPPQDTTF
                     TNLVGQLTYGFEEFKLAPRSCADYQGWDTCTEPVDTDTTTGANVPSTIYEVQMGNVPD
                     KTYVDLKDVVVTSQFFTDAKDNGGFFIAEQAGGEYSGIQVYVYADVAAALTTMNLVPK
                     LGDVVTVSGQYTEFFEYSELTLSKVENLTITGTAAVPDPAVVAPADIATGGAKSEAYE
                     GVLVSVEGVSVTAPANQYGEFTVTGELVVDDLFFAPDPGPEPMSGDMYTRITGLLTYS
                     FEVFKLEPRDLADLQKAP"
BASE COUNT          437 a          882 c         1162 g          531 t
ORIGIN      
        1 gtcagcttgc tcgacaggat gtcgtacgcg gcgtagccgt tggggagggt ctcgacgtcg
       61 ttgaactcgc ggtacaccag ggtggtgttg ttcgaggtgt acgaggccgg gttcttgtag
      121 gcgaacacga agccgggggt caggcgggtg cgctgcaggt agtagtcgac gccggcgacg
      181 acgaaccact ccggcttgac gatcgcgccc tgcgggaagg cgcggtaagg cgcgacgccg
      241 gggatgtcga ggacgacgta gtcgaggtta cgaaacagcg cggtgaagaa gacgcgggtc
      301 ttcttgtagc gcaggcggcc gttgaaggcg ccggccatcg cggggcgaat gaccgtggtg
      361 tccggcttct cccagtcgag cagggtctgc ccgacggtgg tgaactcgaa cgcgcccgag
      421 gcggcgaagc ggttgtcgta ggtctgcgtc tgcgtgagca ggtaggtcgc gtcggggctg
      481 tagcggaaca gctggaagtc gatcgaggtg ccgatcggca tgcccttgcg gaaggtcagg
      541 cgggtcgagc cgccgaaggc ctgggccgtc ttgccgccga tgccggggta cttggtgccc
      601 tgcggaggga aggcgccgcg ctggaagtag ccgccgttga cctcccaggt cagccagcgc
      661 gtgaagctga ggccccagcc gccgagcagg ccgtagtagg tctgcggctc gttgatctgc
      721 tcgttgagca ggcgcgccgt cttggcgccg ccccatacgt agccgtcctt gccggtgccg
      781 accttgaagt cgtagcgcag gcgcatgccg gggaccatcg tgttgctgcg cgccgcgtcg
      841 ccgccggggg cgttgttcgg gaaggtgcgc tcgccgcccc acgtgatgtt gtaggtgtag
      901 ccgagcagca tgcgctgcga gtcgaagggg aacatggtca gcgagacctg gtccccgttg
      961 tagtcgctgc gcttggtgta ccagttgagc ttgatgtagc tgccgtcgtc gcggatctgc
     1021 gtctcggtct tgtacgtctt ctcgttggtg tagtgctgga gctcgacgac gaaggccgcc
     1081 tcggcgtcca tgcgcttgaa gtaggtcggg acgcgcttgt agacgacgag ctgcgtctcg
     1141 gtctcgaagc cgcgcttgcg ctgctcgatc gcgtcgtaga acaggcggtc ggcggcgagc
     1201 ttgaagcccg cgcccggcga ccggtcctgc gcgcccgcga ggatgttgtc gtcagcgatc
     1261 gcaaaggtga cgcgggtcgc catcgcgttg cccgaggtcc acgcgttgaa gccgaccggc
     1321 ggaccctgga tgttgcgcgc gatctcctcg cggttgcggt cgacgcccat ctccgagagc
     1381 ctctgttcag tggcctcgag gcgcacctgc atgcgcgcga gctccgactg caagcgccgc
     1441 aggtcggccg cgtgcttctc ggtctcgctc gggccctcgg tgacgggcgc cgcggggtcg
     1501 atcggcggcg tctggttcgg cgtcggtgac ggcgccgtcg ccgtcggggt gccgaccggg
     1561 atgctcgcgg gcgtggtcga ggccacgtcc ggggcggtgg acggtgagct ggtggtctgg
     1621 ccggagttgg taccctgcgg gccagggacc tgcgcgaacg cctgcgcgct cggcaagatc
     1681 cagatcgcgg cgaggaccgc gagggtggga cacttcatcg actcggctcc agtctgtgga
     1741 cgcgacgaga gggggatggg cgagcgcgca ggcccgctca gggggccttc tgcagatccg
     1801 cgaggtcgcg cggctcgagc ttgaagacct cgaaggagta cgtcaaaagc cccgtgatgc
     1861 gggtgtacat gtcgccgctc atcggctcgg ggccggggtc gggcgcgaag aacaggtcgt
     1921 cgacgacgag ctcgccggtg accgtgaact cgccgtactg gttggccggc gcggtgacgg
     1981 agacgccctc gacggacacg agcacgccct cgtaggcctc ggacttggcg ccaccggtgg
     2041 cgatgtcggc gggggcgacg acggcggggt cgggcacggc ggcggtgccg gtgatcgtca
     2101 ggttctcgac cttggacagc gtgagctccg agtactcgaa gaactcggtg tactgacccg
     2161 agaccgtgac gacgtcgccg agcttgggca cgaggttcat cgtggtcagg gcagcggcga
     2221 cgtcggcgta gacgtagacc tggatgcccg agtactcgcc accggcctgc tcggcgatga
     2281 agaagccgcc gttgtccttg gcgtcggtga agaactgcga ggtgacgacg acatccttga
     2341 gatcgacgta ggtcttgtcg ggcacgttgc ccatctggac ctcgtagatc gtgctgggca
     2401 cgttggcgcc ggtggtcgtg tcggtgtcga ccggctcggt gcaggtgtcc cagccctggt
     2461 agtcggcgca cgagcgtggg gcgagcttga actcctcgaa gccgtaggtg agctgaccga
     2521 ccaggttggt gaaggtcgtg tcctgcggcg gcttggggcc ggggttgggg atgaagaaca
     2581 ggtcgtcgac ctgcaggacg ccgtcgacct ggaactcgcc gaagtcggcg accggcgcgg
     2641 tgaccttggc cccggcgatc tggatcaggc agccctcgaa gtcctcggcc ttggggccac
     2701 cggtggcgac gtcggcggcg gtgacggtcg tcggcgcggg tacggtgccg gcgccggtga
     2761 tcttgaggtc gacgccggcg ctgagggtca gctcggagta gtcgtagaac tcggtgtagg
     2821 tggcgcgcag gtcgatcttg tcgccgaccg cgggcaggct gccttcggcg tcgagctcgg
     2881 cgatcacgtc cgcgtaggcg tagacgagga tgccggagaa cggcccacca ccggcctcgg
     2941 agacgaagaa gttgccgttg ctcttcttgt cgtaatagat cggcgaggtg acgacgaggt
     3001 ccttgaggtc ga
//
LOCUS       DASWNJ010001578         6174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_487580, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001578 DASWNJ010000000
VERSION     DASWNJ010001578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487580"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1189)
                     /locus_tag="VGB85_26195"
     CDS             complement(152..1189)
                     /locus_tag="VGB85_26195"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY0137614.1"
                     /translation="MARGEGGDVEAATSAVVGELEAVLADPEATWKRQLGALLELARG
                     QEREQWELAEVLRRVTELAVETLGVERMGAWLFDADRTVLEAADVYDAATRTHTSGSR
                     LVRGDFPNYFGLLADARVIAVRDACHDPRSAEFAGIYFAPHGVVSTLDAPVRWQGAPV
                     GVLCCEHTGTLRSWRDDEISFIASLADAVSLAFEADRRRQAERALAQKLALIEAQQAA
                     LQRMASPVLEVWDGVLAVPLAGGFDAARRALLTDVLVAAIRERHAAFVLLDLTGVDIV
                     GPDTAEALVRMTRAAGVCGAQCLLSGIHGEVAQTMGSLHAELRGLVTTSSLKTGLQHC
                     LQRATRHTGAA"
     gene            1500..1868
                     /locus_tag="VGB85_26200"
     CDS             1500..1868
                     /locus_tag="VGB85_26200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137615.1"
                     /translation="MKAPATGSDDALRQIYKSTRGYCHACRRKLAFTAYARGVGRGAW
                     DIDVSALRPACRDCLRGHVPQAREHVTARAPVTPEVRGAGCRHTLLGGLLGGFVLGPW
                     GALLGGLLGAEVDGGRVNSD"
     gene            1903..2319
                     /locus_tag="VGB85_26205"
     CDS             1903..2319
                     /locus_tag="VGB85_26205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137616.1"
                     /translation="MARRTFACGHQGKGQFCHRCKQTEAQAAAQQHETAAQRAAHQRE
                     REAWEASFAADPIDLRDLQGRERVQKAREILRRLAAGEPYTSLGGKRWESDRNIVSVP
                     VGRGHRLVLRDEQGKLRPLACMTHEAYNGLKPGNFG"
     gene            complement(2329..2508)
                     /locus_tag="VGB85_26210"
     CDS             complement(2329..2508)
                     /locus_tag="VGB85_26210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137617.1"
                     /translation="MHSPRPSAPTIARYLLDVTNARDARIFGVGRGKGQRVAAEDGPY
                     GPVLRWAGYRLVRTS"
     gene            complement(2533..2925)
                     /locus_tag="VGB85_26215"
     CDS             complement(2533..2925)
                     /locus_tag="VGB85_26215"
                     /inference="COORDINATES: protein motif:HMM:NF036804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HEY0137618.1"
                     /translation="MRPCPCGLPGAFEACCGRYISGARRPDTAEQLMRSRYTAYVKGA
                     VDYLIETTAAASRGVIDRPGLIAYCRTLRGVSLKIVETVDGGPLDERGVVAFEATLRA
                     AGTKLVQRERSRFVVEDGRWVYVDGEVA"
     gene            2975..3916
                     /locus_tag="VGB85_26220"
     CDS             2975..3916
                     /locus_tag="VGB85_26220"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEY0137619.1"
                     /translation="MTHTTRPGRTSTLLPGALGLTLGACHPPAPPQCSECAEHEAEIA
                     GWRAQRLAELRGDQGWLTLAGLYWLREGEQRLGSAADADLQFPAGAPAEVGTVTLQDG
                     VVQLHVKPGVDARIGDAVVRDTPLRSDADPTQPPDRVTIAGRFTFLILSRGDRLGLRL
                     YDVQSPARQNFTGIDTYPVADKWRITARFEPYPTPRLVDHPTVIGTTKAELPGVAVFT
                     LDGAEQRLTPIREHGPHGDELLFVFRDGTSDGETYPGGRFLVTALPKDSAFELNFNKA
                     HNPPCAFTPYATCPLPLPENRLAIRVEAGEKTPAGHD"
     gene            complement(3920..5101)
                     /locus_tag="VGB85_26225"
     CDS             complement(3920..5101)
                     /locus_tag="VGB85_26225"
                     /inference="COORDINATES: protein motif:HMM:NF018880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY0137620.1"
                     /translation="MSLSILLCACGDDGATNTAAATTTGDTTAATTTGASAAESSGST
                     TGAQPTTSGGSEASATDTTGPGPTSSATSTTDATDATDATDTTNTTGPGETTDDTTTG
                     GGVTVYDPNVDGPWQVEHVAGDHMVDGVKIPMDAYYPAGGPEPGPHPVVLISHGFQLP
                     PSQYTSYAQRLASHGYVALTVDFKAGLFDPNHVVYAKQVLGGIDWVAQEATLGPISDT
                     DNVGLTGHSLGGKLSVIAASMDTRVRASITLDPVDGAMNCDPQKCPDASELLPLDIPL
                     GFVGETLDAMGGFMSCAPAADNFLTFYKNTTAPSLAVTVLGANHMSVLDDLAGCGFTC
                     SLCNQPTLDNAVVNDLSRAFVVAFYGRHLRGISDYDLYLTGAEAQARYVDMGLVEIAS
                     K"
     gene            complement(5209..>6174)
                     /locus_tag="VGB85_26230"
     CDS             complement(5209..>6174)
                     /locus_tag="VGB85_26230"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0137621.1"
                     /translation="REVSLASGARLRGRFVVVALGSLLAPPPGMLGHAHLLGYKFAHE
                     LVAAQRKLWDRLERPGAPHVVVIGGGISGVELAGDLAKLSKQSPPGQGLTVTLVDGGD
                     RLLRGLSDRAGRMAARQLERQGVHLRLRTRVLEVGVDSMRLHTADGEPDALRHDGAFW
                     AGGIRAAPVLAQLGLPTIRGYLAVAPTLQCGGAPQVFACGDAARVVDDHGEWPTMQRA
                     IECIWEAGVVARNILALAAAEDRAPVLRRLKLRRDFPHGVSIGAASMIVYGWLAVELG
                     FFGVWFRRFLMQMYFRRYGAPGGSGPARRPAVANADADAAAAPGA"
BASE COUNT          902 a         2335 c         2130 g          807 t
ORIGIN      
        1 tcgccgagct gcgccgcggc ctgcccgcct cgggcccgct cgagctcgcc gcctgggtcc
       61 gcgccgcgct cgcgggcgag gccggcgacg ccccgcccga tcccgccgcg cccgcgaccg
      121 agcacctgcc atgaaaagcg agtaggacag gtcaggccgc gccggtatgc cgcgtcgcgc
      181 gttgcaggca gtgctgcagg ccggtcttga ggctgctggt ggtcaccagc ccgcgcagct
      241 cggcgtgcag cgagcccatc gtctgcgcga cctcgccgtg gatccccgag agcaggcact
      301 gcgcgccgca caccccggcc gcccgggtca tgcgcacgag cgcctcggcg gtgtcgggcc
      361 cgacgatgtc gacgccggtc aggtcgagca gcacgaacgc ggcgtgacgc tcgcggatcg
      421 ccgcgacgag cacgtccgtc agcaaggccc gccgcgccgc gtcgaagccg ccggccagcg
      481 gcaccgcgag cacgccgtcc cagacctcga gcaccggtga ggccatgcgc tgcaacgcgg
      541 cctgctgcgc ctcgatcagc gccagcttct gcgccagcgc gcgctcggcc tgacgccggc
      601 ggtcggcctc gaacgcgagc gagaccgcgt cggccagcga ggcgatgaag ctgatctcgt
      661 cgtcccgcca gctgcgcagc gtccccgtgt gctcgcagca cagcaccccg accggcgcgc
      721 cctgccagcg caccggcgcg tcgagggtcg acacgacccc gtgcggcgcg aagtagatcc
      781 cggcgaactc ggccgagcgc gggtcgtggc acgcgtcgcg caccgcgatc acccgggcgt
      841 cggcgagcag cccgaagtaa ttcgggaagt caccacgcac gagccgcgag ccggaggtgt
      901 gcgtgcgcgt cgcggcgtcg tacacgtcgg ccgcctcgag cacggtgcgg tcggcgtcga
      961 acagccacgc gcccatgcgc tcgaccccga gcgtctcgac cgcgagctcg gtgacgcggc
     1021 gcagcacctc ggcgagctcc cactgctcgc gctcctgccc gcgcgccagc tcgagcagcg
     1081 cgccgagctg ccgcttccag gtcgcctcgg ggtcggcgag cacggcctcg agctcgccga
     1141 cgacggcgga agtcgcggcc tcgacgtcac cgccctcccc gcgcgccacc gccacgatcc
     1201 tcgctgcccg ttctcgaagc tccatgcgca cgtcgaagct accgcgccgc cgccgcgcga
     1261 tcgaggggac agaacgccga tccgcgcggt cgccatgcgc gcccctgcag gcgtccgcag
     1321 ccgcgcgggg ccgatacgcg ccgcctgtcc ccggggtcat gtccgccagc cccgcgagcg
     1381 agcctccggc gcgcctcgag atcccacgaa ttcgggctct cacgccggca attcgccgtt
     1441 ctacggcttg ctccggcaca gatcaggtgg cccgcgggac ccgtgatacg cacgcgtgta
     1501 tgaaggctcc ggcgaccggc agcgacgacg cgctgcggca gatctacaag tcgacccgcg
     1561 ggtactgcca cgcctgtcgc cgcaagctgg cgttcaccgc ctacgctcgc ggcgtcggtc
     1621 gcggcgcgtg ggacatcgac gtctccgcgc tgcgtccggc ctgccgcgac tgtctccggg
     1681 gacatgtccc gcaggccagg gagcacgtga cggcgcgggc cccggtgacg ccggaggtcc
     1741 gcggcgccgg ctgccggcac acgctgctcg gcggcctgct cggcggcttc gtgctcggcc
     1801 cgtggggcgc gctgctcggc ggcctgctcg gcgccgaggt cgacggcggg cgagtgaaca
     1861 gcgactgacg gcgcgggcag tgaatggtcg ggaggtggga tcatggcgag acgaacgttc
     1921 gcgtgcggac atcaaggcaa gggacaattt tgtcaccgct gcaagcagac ggaggcgcag
     1981 gcggccgcgc agcagcacga gacggcggcg cagcgggccg cgcatcagcg cgagcgcgag
     2041 gcctgggagg cgagcttcgc cgccgacccg atcgacctgc gcgacctgca aggccgcgag
     2101 cgcgtgcaaa aggcgcggga gatcctgcgc cggctcgccg ccggcgagcc ctacacgagc
     2161 ctcggcggca agcgctggga gagcgaccgc aacatcgtca gcgtgccggt cggtcgcggc
     2221 catcgcctgg tgctgcgcga cgagcagggc aagcttcgcc cgctcgcctg catgacccac
     2281 gaggcctaca acggcctcaa gcccggcaac ttcgggtgag cgctgccatc acgaggtgcg
     2341 gaccagacgg tacccggccc agcgcaatac cgggccgtac gggccgtcct cggccgcgac
     2401 gcgctgaccc ttgccgcgac cgacgccgaa gatccgcgcg tcgcgtgcat tcgtgacgtc
     2461 gagcaggtag cgcgcgatcg tcggcgcgga gggccgcggc gagtgcacgt gccggcccgc
     2521 gccggtctgc gctcacgcga cttcgccgtc gacgtagacc cagcgcccgt cctcgaccac
     2581 gaagcggctg cgctcgcgct gcacgagctt cgtcccggcc gcgcgcagcg tcgcctcgaa
     2641 cgcgaccacg ccgcgctcgt cgagcggccc gccgtcgacc gtctcgacga tcttcagcga
     2701 gaccccgcgc agggtccgac agtaggcgat caaccccgga cgatcgatca ccccgcgcga
     2761 ggccgccgcg gtcgtctcga tcagataatc gaccgcgccc ttgacgtagg cggtgtagcg
     2821 gctgcgcatc agctgctcgg cggtgtcggg ccggcgcgcg ccgctgatgt agcgcccaca
     2881 acacgcctcg aaggcgccgg gcaggccgca ggggcaaggt cgcatgccgc gagcgtgcca
     2941 cgagcctgcg atccgcgggt agactctgcc cgccatgacc cacacgaccc ggcccgggcg
     3001 cacgtcgacc ctcctgcccg gcgcgctcgg cctgacgctc ggtgcctgcc acccacccgc
     3061 gcccccgcag tgctccgagt gcgccgagca cgaggccgag atcgccggct ggcgcgcgca
     3121 gcgtctcgcc gagctgcgcg gcgatcaggg ctggctcacg ctcgccggcc tgtactggct
     3181 ccgcgagggc gagcagcgcc tcggctcggc cgccgacgcc gacctccaat tccccgccgg
     3241 ggccccggcc gaggtcggga ccgtgacgct gcaagacggc gtggtgcagc tgcacgtcaa
     3301 gcccggcgtc gacgcgagga tcggcgacgc cgtcgtccgc gacacgccgc tgcggtccga
     3361 cgccgacccc acgcagccgc cggaccgcgt cacgatcgcc ggacgcttca cctttttgat
     3421 cttgtcgcgc ggcgatcgcc tcggcctgcg cctgtacgac gtgcagtcgc ccgcgcgcca
     3481 gaactttacc ggcatcgaca catatccggt cgccgacaaa tggcgcatca cggcgcgctt
     3541 cgagccctac cccaccccgc gcctcgtcga tcatccgacc gtcatcggca ccacgaaggc
     3601 cgagctgccc ggcgtcgcgg tcttcacgct cgacggcgcc gagcagcgcc tgaccccgat
     3661 ccgcgagcac ggcccgcacg gcgacgagct gctgtttgta tttcgcgacg gcaccagcga
     3721 cggcgagacc taccccggcg gccgcttcct ggtcaccgca ttaccgaaag acagcgcctt
     3781 cgagctcaac ttcaataagg cacacaaccc gccctgcgcc ttcacgccgt acgcgacctg
     3841 tccgctgccg ctgccggaga accgcctcgc gatccgcgtc gaggccggcg agaagacccc
     3901 cgccggccac gattgaggct cacttcgagg cgatctcgac gaggcccata tcgacgtagc
     3961 gcgcctgcgc ctcggcgcca gtcaaataca ggtcataatc cgagatccca cgcaggtggc
     4021 ggccatagaa ggcgaccacg aaggcgcgcg acaggtcgtt gacgaccgcg ttgtcgaggg
     4081 tgggctgatt acacaaggag caggtgaagc cgcagccggc gaggtcgtcg aggacgctca
     4141 tgtgattggc cccgagcacc gtgacggcga gcgacggggc ggtcgtattt ttataaaaag
     4201 tcaggaaatt gtcggccgcg ggcgcgcacg acataaagcc gcccatcgcg tcgagggtct
     4261 cgccgacaaa gccgagcggg atgtcgagcg gcagcagctc gctggcgtcg gggcactttt
     4321 gcgggtcgca gttcatcgcg ccgtcgaccg ggtcgagggt gatgctggcg cgcacgcgcg
     4381 tgtccatgct cgcggcgatg accgacagct tgccgccgag cgagtgcccg gtcaggccga
     4441 cattgtcggt gtccgagatc gggccgagcg tggcctcctg cgcgacccag tcgatgccgc
     4501 cgagcacctg cttggcgtac acgacgtggt tcgggtcgaa cagaccggcc ttgaagtcga
     4561 ccgtcagcgc gacgtacccg tggctcgcca gccgctgcgc atagctggtg tactgcgagg
     4621 gcgggagctg aaagccgtga gagatcagca cgaccggatg gggccccggc tcgggaccac
     4681 cggccggata gtacgcgtcc atcgggatct tcacgccgtc gaccatgtga tcgcccgcga
     4741 cgtgctcgac ctgccacggg ccgtcgacgt tcgggtcgta caccgtgacg cccccgccag
     4801 tcgtcgtgtc gtccgtggtc tcacccgggc cggtcgtgtt cgtggtgtcc gtcgcgtcgg
     4861 tcgcgtcggt cgcgtcggtc gtgctcgtcg cgctgctcgt cgggcccggc ccggtcgtgt
     4921 ccgtcgcgct cgcctcgctg ccgccgctgg tcgtcggctg cgcaccagtg gtcgacccgc
     4981 tcgactccgc cgcgctcgcg ccggtcgtgg tcgcagcggt ggtgtcaccc gtggtcgtcg
     5041 cggccgcggt gttggtcgcg ccatcatccc cgcaggcaca caacaggatc gaaagagaca
     5101 acgaagatcg acgtgtaggc atgggtagga gcatgcccaa gcgcgggcat ttgcgcgcga
     5161 agatcgaccg cgctcgtgcc gctgatgctc atgagccgtg ccggtgtctc atgcccccgg
     5221 cgcagcggct gcgtccgcgt ctgcattcgc tacggccggc cgacgtgccg gccccgaccc
     5281 tcccggggcg ccgtagcggc gaaaatacat ctgcatcaag aacctgcgaa accatacgcc
     5341 gaagaacccg agctcgaccg ccagccagcc gtacacgatc atcgaggcgg cgccgatgct
     5401 gacgccgtgg gggaagtcgc gccgcagctt gagtcgtcgc aacaccggcg cccggtcctc
     5461 ggccgccgcg agtgccagga tattgcgcgc gacgaccccg gcctcccaga tgcattcgat
     5521 cgcccgctgc atcgtcggcc actcgccatg gtcgtcgacg acccgcgccg cgtcgccgca
     5581 agcgaagacc tgcggagcac cgccgcactg caaggtcggc gcgaccgcga gatatccgcg
     5641 gatcgtcggc aggccgagct gcgcgagcac cggagccgca cggatcccgc cggcccagaa
     5701 cgcaccgtcg tggcgcagcg cgtccggctc gccgtcggca gtgtgcaggc gcatgctgtc
     5761 gaccccgacc tcgagcacgc gcgtgcgcag ccgcagatgc acgccctgcc gctcgagctg
     5821 ccgcgccgcc atgcgcccgg cccggtcgct caggccgcgc agcagtcggt cgccgccgtc
     5881 gacgagtgtc acggtcaacc cctgccccgg cgggctctgt ttggacagct tcgccaggtc
     5941 cccggcgagc tccaccccgc tgatgccccc accgatgacg accacgtgcg gcgccccggg
     6001 ccgctcgagg cggtcccaca gcttgcgctg cgccgccacg agctcgtggg caaacttgta
     6061 gccgagcagg tgcgcgtggc ccagcatgcc cggcggcggg gccagcagcg acccgagcgc
     6121 gaccacgacg aagcgcccgc gcagccgcgc cccggaggcc agcgacacct cccg
//
LOCUS       DASWNJ010001579         2368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_697628, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001579 DASWNJ010000000
VERSION     DASWNJ010001579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697628"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="VGB85_26235"
     CDS             <1..1027
                     /locus_tag="VGB85_26235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HEY0137622.1"
                     /translation="TNYRHVLVNPLKIDWLTRAANYKEVITQAVDAFEADGRAFVTEY
                     AGPSAVVNAAAVYSASWDDTSFIGLDPTEVVNTLNAQGLGSCFDSFSCFWNHPLVFPL
                     LAEFLPAPADVDPAEFYADLGNYAAQIDAAKWGNGDAFAAALLKRVIEPGMHADELLK
                     TWPYLTRMYTTISPSEMMEDPIFHINPDLADVPNLLTANNYVLCNNDSVVTIPDGREF
                     YIPGGQTWPAIPGEEWFAEEVQTVAIKGAPMTLVNNTAAITKKLTEWNLSHGWPYVPG
                     ETEGTPTTSDTEGGTEGTGGDTEGTAGGNDGDEGCGCRSDEQDGRQGALWLGLAALGL
                     LGVRRRR"
     gene            complement(1109..1888)
                     /locus_tag="VGB85_26240"
     CDS             complement(1109..1888)
                     /locus_tag="VGB85_26240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137623.1"
                     /translation="MTEDIKETDEKAASRRKRRGTGPISTEEERLLATAKPIAKVPPR
                     GGGSEGSVVKPKFGRYERADFERVETMLAAFANRRLGPLVNVVAKGFFGAATPEEREN
                     SGIRTAFYSFFLYTYRDPKGIRVIDLFAESGLPLEGRQRAALDACLQARLVVMSIEDV
                     HPGKHRMRGRDLLRDEPITALDRNASSLVKPGERLMCWQIPWGTTWQPIGYAVQVEAR
                     KAVALDRGIETLCNGLRAVRRELPDQHAANLWWVVHRVANM"
     gene            complement(1911..>2368)
                     /locus_tag="VGB85_26245"
     CDS             complement(1911..>2368)
                     /locus_tag="VGB85_26245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137624.1"
                     /translation="PYEGAPLNRPCDVTGLDLGNLVLGATVVTLLDEDGAVVTEVMLA
                     DADMDEDASFPLEATCRDFDRVRNTTEIPWSAAAGQRAYSIKIETWAAADDPMVAAPC
                     FSNGAAPMQLAPGISPAIVVPRTRTDGACADCAVGEGDCSRCEDRVCKL"
BASE COUNT          373 a          803 c          848 g          344 t
ORIGIN      
        1 gaccaactac cggcacgtgc tggtcaaccc gctcaagatc gactggctga cgcgcgcggc
       61 gaactacaag gaagtgatca cgcaggcggt cgacgcgttc gaggccgacg gccgcgcctt
      121 cgtgaccgag tacgcgggcc cgagcgccgt cgtcaacgcg gcggcggtgt acagcgcgag
      181 ctgggacgac acgagcttca tcggcctcga cccgaccgag gtggtcaaca cgctcaacgc
      241 ccagggcctc ggcagctgct tcgacagctt tagctgcttc tggaaccacc cgctggtctt
      301 cccgctgctg gccgagttcc tgccggcgcc ggcggacgtc gacccggcgg aattctacgc
      361 ggacctcggc aactacgcgg cgcagatcga cgcggcgaag tggggcaacg gcgacgcgtt
      421 cgcggcggcg ctgctcaagc gggtgatcga gccgggcatg cacgcggacg agctgctcaa
      481 gacgtggccg tacctgacgc gcatgtacac gacgatctcg ccgagcgaga tgatggagga
      541 cccgatcttc cacatcaacc cggacctcgc ggatgtgccg aacctgctga cggccaacaa
      601 ctacgtgctg tgcaacaacg acagcgtggt gacgatcccc gacggccgcg agttctacat
      661 ccccggcggc cagacctggc cggcgatccc gggcgaggag tggttcgcgg aggaggtcca
      721 gacggtggcg atcaagggcg cgccgatgac tctggtcaac aacaccgccg cgatcaccaa
      781 gaagctgacg gagtggaacc tctcgcacgg ctggccgtac gtgcccggcg agaccgaggg
      841 caccccgacg accagcgaca ccgaaggcgg caccgagggc accgggggcg acaccgaggg
      901 taccgcgggc ggcaacgatg gcgacgaggg ctgcggctgc cgcagcgacg agcaggacgg
      961 ccgccagggc gcgctgtggc tcggcctcgc ggccctcggg ctgctcggcg tgcggcgccg
     1021 tcgctagccg atgacggaca tgtcctccgg gacatgttca aaaggcccgg tgagctgcgc
     1081 tcgccgggcc ttttcataat tttcgcgctc acatgttggc gacgcgatgc acgacccacc
     1141 acaggttggc ggcgtgctgg tcgggcagct cgcggcggac cgcacgaagg ccattgcaaa
     1201 gggtctcgat gccgcggtcg agcgcgacgg ccttgcgcgc ctcgacctgc acggcgtagc
     1261 cgatcggctg ccacgtcgtg ccccagggga tctgccagca catcaggcgc tcgccgggct
     1321 tgacgaggct cgacgcgttg cggtcaagtg cggtgatcgg ctcgtcgcgc aggaggtccc
     1381 ggccgcgcat gcggtgcttg cccgggtgca cgtcctcgat gctcatgacg accagccgcg
     1441 cctgcaggca agcgtcgagc gcggccctct ggcggccctc gagcggcagg cccgactcgg
     1501 cgaacaggtc gatgacgcgg atccccttgg ggtcgcggta ggtatagagg aagaagctgt
     1561 agaacgcggt gcggatgccc gaattctcgc gctcctccgg cgtcgcggcc ccgaagaagc
     1621 ccttggcgac gacgttgacc agcggaccga gccggcgatt ggcgaacgcg gcgagcatcg
     1681 tctcgacgcg ctcgaagtcg gcgcgctcgt agcggccgaa cttcggcttg accaccgagc
     1741 cctcgctgcc accgccgcgc ggcgggacct tggcgatcgg cttcgcggtc gccagcaggc
     1801 gctcctcctc ggtcgagatc ggcccggtgc cccgtcgctt gcggcgcgag gccgccttct
     1861 catccgtctc tttgatgtct tccgtcatcg atcgatccca tcgcctcgcg ctagagctta
     1921 caaacgcggt cctcgcagcg cgagcagtcg ccctcgccga cagcacagtc ggcgcaggcc
     1981 ccatcggtgc gcgtgcgcgg cacgacgatc gcggggctga tgccgggggc gagctgcatc
     2041 ggcgcggcgc cgttcgagaa gcagggcgcg gcgaccatcg ggtcgtcggc cgcggcccag
     2101 gtctcgatct tgatcgagta agcgcgctgg cccgccgccg cgctccacgg gatctccgtg
     2161 gtgttgcgga cgcggtcgaa gtcgcggcag gtcgcctcga gcgggaagct ggcgtcctcg
     2221 tccatgtcgg cgtcggcgag catgacctcg gtgacgacgg cgccgtcctc gtcgagcagc
     2281 gtgacgacgg tggcgccgag gacgaggttg ccgaggtcga ggccggtgac gtcgcagggg
     2341 cggttgaggg gggcgccctc gtaaggct
//
LOCUS       DASWNJ010001580         3582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_28367, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001580 DASWNJ010000000
VERSION     DASWNJ010001580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28367"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VGB85_26250"
     CDS             complement(<1..889)
                     /locus_tag="VGB85_26250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137625.1"
                     /translation="MIARSSRGFHLRPLHRAALGVWALALAGCVQYEKDDEGGPTGDE
                     APADGGGATTGSPTGAPTGAPTTEDTPTTGGEGSQCDLWAQDCPGGSKCMPYDQDNDG
                     IHDSARCVPVDDSPGQAGDDCTIEGSVASGIDDCDVALMCWNTDNNNQGRCVAMCSGT
                     PGDPSCPEALICDISNGGVLLLCVTPCDPLTPSCPIGQICLPGGDKQFVCDVDASGDQ
                     GGYGDPCAFVNVCDQGLLCVSGQSVPGCKTDGCCTEYCDLSAPDVVCAGAPEQECVSF
                     YDDDAAPPGYENVGVCTIPS"
     gene            1238..2599
                     /locus_tag="VGB85_26255"
     CDS             1238..2599
                     /locus_tag="VGB85_26255"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY0137626.1"
                     /translation="MSDLVGLLWVFVLVLVNGFFVAAEFALVSVRRTRIDQLANEGSS
                     RARLTQSVLKDLDLYIAATQLGITMASLGIGFLAEPALEHLAAPTLLGWGVDPGNVTT
                     ISFVLAFTLSTALHIVFGELAPKSLALQRSEDVSMLVSIPMIGFAIVFRPFIYVLNAT
                     GNGVVRMFGLKPVPGHHSAHSEEEIRMIVSASRQEGVLEEQEKELLNNVFDLSDTPVR
                     AIMTPRVDMIAIEETAPLRRLMTLNEEHGYSRVPIFRDTTDNIVGVAYIGDLLRHLDQ
                     LDTVTVGQIARATVFAPENMKVLDLFPLLQRKKTHLAIVVDEFGGTAGIVTLEDAVEE
                     VVGEIYDENDDEEDEKLFTLLEDGSYLFDATINCTEVEKTLGIDLDDQIQGNYETLSG
                     FLFHQFEYIPRPGEETSAAGWTFHVVEADDRRVLKIRASRQEAESTSTSGEGAAPPAS
                     ATG"
     gene            2657..>3582
                     /locus_tag="VGB85_26260"
     CDS             2657..>3582
                     /locus_tag="VGB85_26260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137627.1"
                     /translation="MRRSIMALSLALVACNPPKSGDDPEDGATTSTSTTHGTDGHSSA
                     PTTGGSANGSGGDDGPVTTSATTSLPPSTTSPPDLTTSTDGSPPTTTDSATASASDPG
                     GASVPFFPEETDGSFLLTPDVPKGECNFWNEDCPAGQKCMPFASEGAPSWDSLGCVPL
                     VVDPAPVGAPCVAIGHAASGLDSCATHAICWGVDADLQGECVSLCFGDELDHLCKLST
                     ETCVLANDKLLAVCLPKCDPLISDCPADQVCIPSVGEFACAPDVSGPGGGLFSTCAAG
                     NACDPGLVCAGSENAAQCPPSSDECCLVYCDL"
BASE COUNT          529 a         1369 c         1154 g          530 t
ORIGIN      
        1 acgacgggat cgtgcacacg ccgacgttct cgtaccccgg aggcgccgcg tcgtcgtcat
       61 agaacgacac gcactcctgc tcgggcgcgc cggcacacac gacatcgggc gccgagagat
      121 cgcagtactc ggtgcagcag ccgtcggtct tgcagcctgg caccgactgc cccgacacgc
      181 acagcaggcc ctggtcgcac acgttgacga acgcgcaggg gtcaccgtag ccgccctgat
      241 ccccgctcgc gtcgacgtcg cacacgaatt gcttgtcgcc gcccggcagg cagatctgtc
      301 cgatcggaca ggagggcgtc agcgggtcgc acggcgtcac gcacaacagc agcacgccgc
      361 cgttgctgat gtcgcagatc aacgcctcgg ggcagctcgg gtcgcccggc gtgccgctgc
      421 acatcgccac gcagcggccc tggttgttgt tgtccgtgtt ccagcacatc agggcgacgt
      481 cgcagtcgtc gatcccgctg gcgaccgacc cctcgatcgt gcagtcgtcg cccgcctggc
      541 ccggcgagtc gtcgaccggc acgcagcgcg ccgagtcgtg gatgccgtcg ttgtcctggt
      601 cgtagggcat gcacttgctg cccccggggc agtcctgcgc ccacaggtcg cactgcgacc
      661 cctcgccgcc ggtcgtcggc gtgtcctcgg tcgtcggtgc gccagtcggt gcgccggtcg
      721 gcgagccggt cgtcgcgccg ccgccgtcgg ccggcgcctc gtcgccggtc gggccgccct
      781 cgtcgtcctt ctcgtattgc acgcagccgg cgagggcgag cgcccacaca ccgagcgcgg
      841 cgcggtggag aggacgcagg tgaaagcccc gggacgaacg cgcgatcacc cgcggaccct
      901 aacacgcccg tacgcggccc cgcgagccgc caaagccggg ttgcgcgggc ccgccagccc
      961 gcccggcacg acctgcgcag atcgcgcgta cgtggggaag cgctcgtagc ggcccctggg
     1021 agcccgaccg tgcggggggc tccgcgagcg cgcgcaccgg acaaagcttg ctacaatcgc
     1081 cggccttgaa cgcagagcct cccagttgtc gccgaccccg gccgtcccgg ccgccgtcgc
     1141 gctcgtcctc ctccggatcc ggcccgactc cctcggccgc cccggcatcc ccggcatccc
     1201 gcccgtcctt cgccgtcgtt gaggagcgcg cgcagcgatg agtgacctgg tcggactact
     1261 gtgggtcttc gtgctcgtgc tggtcaacgg cttcttcgtc gcggccgagt tcgccctcgt
     1321 cagcgtgcgc cgcacccgca tcgaccagct ggccaacgag ggcagctcgc gggcccggct
     1381 gacgcagtcg gtcctcaagg acctcgacct ctacatcgcg gccacccagc tcggcatcac
     1441 catggccagc ctcggcatcg gcttcctcgc cgagccggcg ctcgagcacc tcgccgcgcc
     1501 gaccctgctc ggctggggcg tcgaccccgg caacgtgacc acgatctcct tcgtgctcgc
     1561 gttcaccctg tcgaccgcgc tgcacatcgt gttcggcgag ctcgcgccca agtcgctggc
     1621 gctgcagcgc tccgaggacg tctcgatgct ggtctcgatc ccgatgatcg gctttgcgat
     1681 cgtgtttcgc ccgttcatct acgtgctcaa cgcgaccggc aacggcgtcg tccgcatgtt
     1741 cggcctcaag cccgtgcccg gccaccacag cgcgcactcc gaggaggaga tccgcatgat
     1801 cgtgtccgcc tcgcggcaag agggcgtgct cgaggagcag gagaaggagc tcctcaacaa
     1861 cgtcttcgac ctctcggaca cgccggtgcg cgccatcatg accccgcgcg tcgacatgat
     1921 cgcgatcgag gagaccgcgc cgctgcgccg gctgatgacc ctcaacgagg agcacggcta
     1981 ctcgcgggtc ccgatcttcc gtgacaccac cgacaacatc gtcggcgtcg cgtacatcgg
     2041 cgacctgctg cgccacctcg accagctcga caccgtcacc gtcggccaga tcgcccgcgc
     2101 caccgtgttc gcgccggaga acatgaaggt cctcgacctg tttccgctgc tgcagcgcaa
     2161 aaagacccac ctggcgatcg tcgtcgacga gttcggcggc accgccggca tcgtgaccct
     2221 cgaggacgcc gtcgaggagg tcgtcggcga gatctacgac gagaacgacg acgaggagga
     2281 cgagaagctg ttcaccctgc tcgaggacgg cagctatctg ttcgacgcga cgatcaactg
     2341 caccgaggtc gagaagaccc tcggcatcga cctcgacgac cagatccagg gcaactacga
     2401 gactctctcg ggcttcctgt tccaccagtt cgagtacatc ccgcgccccg gcgaggagac
     2461 ctcggccgcc ggctggacct tccacgtcgt cgaggccgac gaccgtcgcg tgctcaagat
     2521 ccgcgcgtcc cggcaggaag ccgagagcac cagcaccagc ggcgagggtg ccgcgccgcc
     2581 cgcctccgcg accggctgac gtggtccgat ccgggtcggt ctccatctgc gcccgaccgt
     2641 gatgtactgg gccgacatgc gtcgatccat catggccctg tccctcgcgc tcgtcgcctg
     2701 caacccgccg aagtcgggcg acgatccaga ggacggggcc acgacctcga cctcgaccac
     2761 gcacgggacc gacggtcact cgagcgcgcc gaccaccggc ggctcggcga acgggtccgg
     2821 tggggacgac ggcccggtca cgacctcggc cacgacctcg ctcccgccgt cgacgacgtc
     2881 gccgccggac ctgacgacct cgaccgacgg gtcccccccg acgaccacgg attccgcgac
     2941 cgccagcgcc agcgaccccg ggggcgccag cgtgccgttc ttccccgagg agaccgacgg
     3001 ctcgttcctc ctcaccccgg acgtcccgaa gggcgagtgc aacttctgga acgaggactg
     3061 ccccgcgggc caaaagtgca tgcccttcgc gtcggagggc gcgccgagct gggactcgct
     3121 cggatgcgtc ccgctcgtcg tcgaccccgc gccggtcggt gctccgtgcg tggcgatcgg
     3181 ccacgccgcc agcggcctcg acagctgtgc gacgcacgcg atctgctggg gcgtcgacgc
     3241 ggacttgcag ggcgagtgcg tctcgctgtg cttcggcgac gagctcgacc acctgtgcaa
     3301 gctctcgacc gagacctgcg tgctggccaa cgacaagctc ctcgcggtct gcctgcccaa
     3361 gtgcgacccg ctgatcagcg actgtcccgc ggaccaggta tgcatcccgt ccgtcggcga
     3421 attcgcctgc gcccccgacg tgagcggccc cggcggcggg ctgttctcga cctgcgccgc
     3481 cggcaacgca tgcgaccccg ggctcgtctg cgccggctcg gagaacgccg cgcagtgccc
     3541 cccgagctcc gacgagtgct gcctcgtata ctgtgacctc ga
//
LOCUS       DASWNJ010001581         4677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_697642, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001581 DASWNJ010000000
VERSION     DASWNJ010001581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697642"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1811
                     /gene="kaiC"
                     /locus_tag="VGB85_26265"
     CDS             <1..1811
                     /gene="kaiC"
                     /locus_tag="VGB85_26265"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF006799.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HEY0137628.1"
                     /translation="YRRRRGHPGSIEAPGMKDIHASEWSGIAKAPTGIHGLDAITGGG
                     LPQGRPTLVCGSAGCGKTLFAMEFLVRGITEFGEPGVFFSFEETPRELAQNVRSLGFD
                     LDDLTSRGLIALEHIRIERDEFDETGEFDLEGLFIRLGLAIDTIGAKRVVLDTVETLF
                     AGLDNEAVVRSELRRLFRWLKDRGVTAVITAERGDGSLTRQGLEEYVSDCVILLDHRV
                     NDQTSIRRLRVVKYRGSSHGTNEYPFLIDASGFSVLPLTSLGLDNEASTERISTGIDR
                     LDVMLGGGPFRGTSVLLSGTAGTGKSSIAAHMAAASCRRGERCLYYSFEESPSQILRN
                     MRSIGLDLAPSVEQGLLSFVSVRPTLYGLEMHLATMHKHICAIDPRLVVVDPISNFIT
                     TGSAREAHTMLLRLVDFLKTRQTTAVFVSLTSGGGAIEATEQNISSLIDTWILLRDIE
                     LGGERNRGLHVLKSRGTAHSNQVREFVLTANGVELRDVYIGPEGVLTGSMRLAQEAKE
                     RAAAQDRQQKIASKRREIERKRHAMEAQLVALRAGFEIAEEELELLIATKEAAGQQYL
                     DDRTDMAQSRRADHEETVTLPPAEPTAITPTPGASI"
     gene            1808..2155
                     /locus_tag="VGB85_26270"
     CDS             1808..2155
                     /locus_tag="VGB85_26270"
                     /inference="COORDINATES: protein motif:HMM:NF019309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HEY0137629.1"
                     /translation="MSSEHDTPGEAKPPPDLEVWEFYLYVAGQRPKSLKAVENLTRMC
                     EEHLAGRYKIEVIDLLRNPGLAKEDEIVALPTLVRKLPEPMRRVIGDLSNLEKALVRF
                     ELPTPKTSLPRLP"
     gene            2152..4167
                     /locus_tag="VGB85_26275"
     CDS             2152..4167
                     /locus_tag="VGB85_26275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0137630.1"
                     /translation="MKDTLDILRGEIEELRRQLAEARETSRAIQAGEVDAVVVIEDEL
                     PRVLMLDSADKPYQLLVERMHQAAATLTLEGALLYCNQAFARLVRVPLERLRGRHMRD
                     LIDARSRPLFDVLLRDALAASVEAEVVLRRDDGSWVPLFLGVNALHEGVGDVCLLVAD
                     LTEQKRRERLVADSAMARSVLEQVAEAVIVCDTAGTVVRASRTAHELCGINPILKPFD
                     EVFALTRPASQAAEDPVDVGAVWRGVSLHGQEFCFHRPGGARAELLLNAGPLVAAGGE
                     TLGAVITLTDITPLRQTQDELHRRTAALQEADVRKDEFLAMLAHELRNPLAPIRASLE
                     IMTLRGLDDPMLRKCRDVIERQTHQLTRLVDDLLEVSRIRTGKIQLRTERVGLAVAVS
                     RGIETSRPVVDARRHQLAVSIPEAPIWVRADLTRIAQVVGNLVNNAAKYTEQGGEISV
                     RVEVEGELAVIRVRDSGIGIPQAMLATVFDLFTQVSGAIDRSQGGLGIGLALVKQLVV
                     MHGGEVEARSEGPGSGTEFIVRLPLWQEEPTGARVVVPDAPAASGKRRRVLVVDDNKD
                     AVDILADLLALLGHDVRTACDGPSAITAAAEFLPEIILCDIGLPLMDGYEVVAELRAR
                     PAFARTRFIALTGYGRDEDRQRSHAAGFHAHLVKPFDLVKLAVLLDA"
     gene            complement(4239..>4677)
                     /locus_tag="VGB85_26280"
     CDS             complement(4239..>4677)
                     /locus_tag="VGB85_26280"
                     /inference="COORDINATES: protein motif:HMM:NF019610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137631.1"
                     /translation="TSDQQYTFGWSTLRDRLGVVGFSRLDPNQYGGGGSVDAPGLLLR
                     RGLRLAIHETAHMFGLGHCQLFRCVMNGISDLAELDATPPRLCPLCLRKLHLVTGLDP
                     RARDAALLRVHEQLGVTEEAQWLAARADHLWRARSMHATGPRR"
BASE COUNT          704 a         1617 c         1685 g          671 t
ORIGIN      
        1 tctaccgtcg cagacgtgga catccaggtt cgatcgaggc gcccggcatg aaagatatcc
       61 acgcgagcga atggtccggc atcgccaagg cgccgaccgg catccacggg ctcgacgcga
      121 tcaccggcgg cgggctgccg caggggcgcc cgaccctcgt ctgtggcagc gccgggtgcg
      181 gcaagacgct gttcgcgatg gagtttttgg tccgcgggat caccgagttc ggcgagccgg
      241 gcgtgttctt ctcgttcgag gagaccccgc gcgagctggc gcagaacgtg cggtcgctcg
      301 gcttcgacct cgacgacctc acctcgcgcg gcctgatcgc cctcgagcac attcgcatcg
      361 agcgcgacga gttcgacgag acgggcgagt tcgacctcga gggcctgttt atccgcctcg
      421 ggctggcgat cgacaccatc ggcgcgaagc gggtcgtgct cgacacggtc gagaccctgt
      481 tcgccgggct cgacaacgag gcggtggtgc gctcggagct gcggcggctg tttcgctggc
      541 tcaaggaccg gggcgtcacc gcggtgatca cggcggagcg cggcgacggc tcgctgaccc
      601 gccaggggct cgaggagtac gtgtccgact gcgtgatctt gctcgaccac cgcgtcaacg
      661 atcagacctc gatccggcgc ctgcgcgtcg tcaagtatcg cggcagctcg cacggcacca
      721 acgagtaccc gttcctcatc gacgccagcg gtttttcggt gctgccgctg acctcgctcg
      781 ggctcgacaa cgaggcctcg acggagcgca tctcgaccgg catcgaccgg ctcgacgtca
      841 tgctcggcgg cgggccgttt cgcggcacca gcgtgctgct gtcgggcacc gcggggaccg
      901 gcaagtcgag catcgccgcg cacatggccg cggcgagctg tcgccgcggc gagcgctgcc
      961 tgtattactc gttcgaggag tcgccgtcgc agatcctccg caacatgcgg tcgatcggcc
     1021 tcgacctcgc gccgagcgtc gagcagggcc tgctgtcgtt cgtgtcggtg cgcccgaccc
     1081 tgtacgggct cgagatgcac ctggcgacga tgcacaagca catctgcgcg atcgacccca
     1141 ggctggtggt cgtcgacccg atcagcaact tcatcaccac cgggtcggcc cgcgaggcgc
     1201 acacgatgct gctgcgcctg gtcgacttcc tcaagacccg ccagaccacc gcggtgttcg
     1261 tcagcctgac cagcggcggc ggggcgatcg aggcgaccga gcagaacatc tcgtcgctga
     1321 tcgacacgtg gatcctgctg cgtgacatcg agctcggcgg cgagcgcaat cgcgggttgc
     1381 acgtgctcaa gtcccgcggc accgcccact cgaaccaggt ccgcgagttc gtgctgacgg
     1441 ccaacggcgt cgagctgcgc gacgtgtaca tcggcccgga gggcgtgctg accggctcga
     1501 tgcggctcgc ccaggaggcc aaggagcgcg ccgcggcgca ggaccgccag cagaagatcg
     1561 ccagcaagcg gcgagagatc gagcgcaagc gccacgcgat ggaggcccag ctcgtcgcgc
     1621 tgcgcgcggg cttcgagatc gccgaggagg agctcgagct gctgatcgcc accaaggagg
     1681 cggccggcca gcagtacctc gacgaccgca ccgatatggc ccagagccgc cgcgccgacc
     1741 acgaggagac ggtcacgctc ccgccggccg aaccgaccgc gatcaccccc accccgggag
     1801 cgtcgatatg agcagcgagc acgacacccc gggcgaggcg aagccaccac cggacctcga
     1861 ggtctgggag ttctacctgt acgtcgccgg ccagcggccc aagtcgctca aggcggtcga
     1921 gaacctgacg cggatgtgcg aggagcacct ggccgggcgc tacaagatcg aggtgatcga
     1981 cctcctgcga aacccggggc tcgccaagga ggacgagatc gtcgcgctgc cgacgctggt
     2041 ccgcaagctg ccggagccga tgcgccgcgt gatcggtgac ctgtcgaacc tcgagaaggc
     2101 gctcgttcgc ttcgagctgc cgaccccgaa gacgtcattg ccacgattgc catgaaggac
     2161 acgctcgaca ttctccgggg cgagatcgag gagctccggc gccagctcgc cgaggcccgc
     2221 gagaccagcc gcgcgatcca ggccggcgag gtcgatgcgg tggtcgtcat cgaggacgag
     2281 ctgccgcgcg tgctgatgct cgactccgcc gacaagccgt atcagttgct ggtcgagcgg
     2341 atgcatcagg ccgccgccac gctgacgctc gagggcgccc tgctgtactg caaccaggcg
     2401 ttcgcgcggc tggtccgcgt gccgctcgag cgcctgcgcg gccgccacat gcgcgacctg
     2461 atcgacgcgc gctcgcggcc gctgttcgac gtgctgctgc gcgacgcgct ggcggcgagc
     2521 gtcgaggcgg aggtcgtgct gcgccgcgac gacggctcgt gggtcccgct gttcctcggc
     2581 gtcaacgcgc tgcacgaggg cgtcggcgac gtgtgtctgc tcgtcgcgga cctgaccgag
     2641 cagaagcggc gcgagcggct ggtcgccgac tcggcgatgg cgcgctcggt gctcgagcag
     2701 gtcgccgagg cggtgatcgt gtgtgacacg gcgggcacgg tggtccgcgc gagtcggacg
     2761 gcgcacgagc tgtgcggcat caacccgatc ctcaagccct tcgacgaggt gttcgcgttg
     2821 acgcgaccag cgtcgcaggc ggccgaggat ccggtcgacg tcggcgccgt gtggcgcggc
     2881 gtgtcgctgc acggccagga gttctgcttt catcgcccgg gtggcgcgcg ggcggagctg
     2941 ctgctcaacg cgggcccgct ggtcgccgcg ggcggcgaga cgctcggcgc ggtgatcacg
     3001 ttgacggaca tcacgccgct gcgccagacc caggacgagc tgcaccggcg gacagccgcg
     3061 ctgcaggagg ccgacgtccg caaggacgag tttttggcga tgctggcgca cgagctgagg
     3121 aacccgctgg cgccgatccg ggcctcgctc gagatcatga cgctgcgcgg gctcgacgac
     3181 ccgatgcttc gcaagtgccg ggatgtcatc gagcggcaga cgcaccagct gacgcggctc
     3241 gtcgacgact tgctcgaggt gtcgcggatc cggaccggga agatccagct gcgcaccgag
     3301 cgggtcggcc tcgcggtcgc ggtctctcgc ggcatcgaga ccagccgccc ggtggtcgac
     3361 gccaggcggc atcaactcgc ggtctcgatc cccgaggcgc cgatctgggt gcgggccgac
     3421 ctgacgcgga tcgcccaggt cgtcggcaac ctcgtcaaca acgccgcgaa gtacaccgag
     3481 cagggcggcg agatctcggt gcgcgtcgag gtggagggcg agctcgcggt gatccgcgtg
     3541 cgtgacagcg ggatcgggat cccgcaggcg atgctcgcca cggtgttcga cctgttcacg
     3601 caggtcagcg gcgcgatcga ccgctcgcag ggcggcctcg ggatcggcct cgcgctcgtc
     3661 aagcagctcg tcgtcatgca cggcggcgag gtcgaggcgc gcagcgaggg gcccggctcc
     3721 ggtacggagt tcatcgtccg cctgccgctg tggcaggagg agccgaccgg cgcgcgggtg
     3781 gtcgtgcccg acgcgccggc ggccagcggc aagcgccggc gcgtgctcgt ggtcgacgac
     3841 aacaaggacg ccgtcgacat cctcgccgac ctgctcgcgc tgctcggtca cgacgtccgc
     3901 accgcctgcg acggcccctc ggcgatcacc gcggccgcgg agttcttgcc ggagatcatc
     3961 ctgtgtgaca tcggcctgcc gctgatggac ggctacgagg tggtcgccga gctgcgcgcg
     4021 cggccggcct tcgcccgcac gcgcttcatc gccctcaccg gctacggccg cgacgaggac
     4081 cgccagcgct cgcacgcggc cggcttccac gcccacctcg tcaaaccatt cgacttggtg
     4141 aagctcgcgg tgctgctcga cgcgtgagca gcgtgcagcg ggttgacgcg ggccgaggcg
     4201 ttggccacgc tggtcgcccg ctgcgcgccg ccctgagctc agcggcgcgg cccggtggcg
     4261 tgcatcgagc gtgcgcgcca caggtggtcg gcgcgcgcgg ccagccactg cgcctcctcc
     4321 gtgacgccga gctgctcgtg gacgcggagc agcgcggcgt cgcgggcccg ggggtcgagg
     4381 ccggtgacga ggtgcagctt gcgcaggcac aatggacata gtcgcggcgg cgtcgcgtcg
     4441 agctccgcga ggtcggagat cccgttcatc acgcagcgaa acagctgaca gtggccgagg
     4501 ccgaacatat gcgcggtctc gtggatcgcc aggcgcaggc cacgacgcag cagcagcccc
     4561 ggcgcgtcga cagagccgcc gccgccgtac tggttcgggt cgaggcggga gaaccccacc
     4621 acgccgaggc ggtcgcgcag cgtcgaccag ccgaatgtat attgctggtc gctggtg
//
LOCUS       DASWNJ010001582         2786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_28406, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001582 DASWNJ010000000
VERSION     DASWNJ010001582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28406"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2786
                     /locus_tag="VGB85_26285"
     CDS             <1..>2786
                     /locus_tag="VGB85_26285"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY0137632.1"
                     /translation="EVKALLARLPPLALDTADVKEFALRPASQPPPRTGATVLASFPT
                     KGTPPAVKPGALTVLRHSPDGEVPVAADLSITFSQPMVAVTSRDTQKAADVPVKLSPA
                     TPGEWQWLGTRTLVFRPTGRFPMATEFRVEVPAGTAAASGGKLSQGTSFTFATPPPTL
                     RSSSPSGVTTRTPKIFLAFDQRVDPTSVLPAIRVAARGGEPAPLRIVPLAEVRKDKQL
                     RGLIESVEDGRWLVVQPTRALPPDSDIQVILAAGLRSAEGPRKTTKPQEFSFHTYGAL
                     ALHSAECGWGQPQCDPGTPFRFVFTNELDTRHFKNEWVRVEPAIPGMQVARYGNELSI
                     QGATKPRTKYTVTIDAALRDAYGQTLGKPEQRSFTVGPAPDSLHALVQQLTVLDPSAP
                     GKLSVFTTGHESLKVQIHAVTPADWDAYLKFLRARNEDTLRRATPPGKPVFSDTVEIK
                     GDPDVLTETAIDLRAALPAGLGHAIVVVTPTKVPKEPWMRHEVITWAQATRIGLDAFV
                     DNEKLVGFATRLADGAAIPNVQLQLLPSGARATTGADGLASLPLPQKGNRVLLATQGQ
                     DRAFLPEQVGWWYGEDEGGWRPYKREDSLHWYVFDDRQMYRPGETAKIKGWLRSRTPG
                     LRGGLQLPTSAVKDLRWTLHDSQGNKLLTGTTKVNALGGFDLSLTLPRTPNLGHAGLT
                     FEVTAGTMKGQSWAHGLQIQEFRRPEYEVKARASEGPHLIGSHADLTVSAQYFAGGPL
                     ANAEVAWQVSASSGSYSPPGHDDYTFGRWTPWWGWGMREIGFPGREYHPPESFAGRTD
                     ASGEHHLRADFLSVDPLEATSLHAEATVLDVNRQAWTSGVDLLVHPASLYVGMKSDRM
                     FVQQGEPLHIDAIVTDIDGKQVQGQDILIRAVRIAWEQVGGEYKETEKDPQDCKQASA
                     AAAVRCSFIPKEGG"
BASE COUNT          453 a         1109 c          873 g          351 t
ORIGIN      
        1 gcgaggtcaa ggccttgctc gcccggctac caccgctcgc cctcgacacc gcggacgtca
       61 aggagttcgc cctgcgcccc gcctcgcagc cgccaccgag gaccggcgcc accgtgctcg
      121 ccagcttccc gaccaagggc acgccgcccg cggtcaagcc cggcgcgctg accgtgctgc
      181 gtcacagccc cgacggcgag gtcccggtcg cggccgacct ctcgatcacc ttctcgcagc
      241 cgatggtcgc cgtcacctcg cgcgacacgc aaaaggccgc cgacgtcccc gtcaagctga
      301 gccccgcgac cccgggcgag tggcagtggc tcggcacgcg caccctcgtg ttccgcccga
      361 ccggccgctt cccgatggcg accgagttcc gcgtcgaggt ccccgccggc acggcagcgg
      421 cgagcggcgg caagctgtcc caggggacat ctttcacctt cgcgaccccg ccgccgacgc
      481 tgcgctcgtc ctccccgtcc ggcgtcacca cgcgcacccc gaagatcttc ctcgccttcg
      541 atcaacgcgt cgatccaacc tcggtcctgc ccgcgatccg cgtcgccgcc aggggtggcg
      601 agccggcccc cctgcggatc gtcccgctcg ccgaggtgcg caaggacaag cagctacgcg
      661 gcctgatcga gtccgtcgag gacggtcgct ggctggtcgt acagccgacc cgcgccctcc
      721 cgcccgacag cgacatccag gtgatcctcg ccgccggtct gcgctccgcc gagggcccgc
      781 gcaagaccac caagccgcag gaattcagct tccacaccta cggcgcgctc gccttgcaca
      841 gcgccgagtg cggctggggt cagccccagt gcgacccggg caccccgttt cgcttcgtct
      901 tcaccaacga gctcgacacc aggcacttca aaaacgagtg ggtccgcgtc gagccggcga
      961 tcccgggcat gcaggtcgcc cgctacggca acgagctctc gatccagggc gcgaccaaac
     1021 cccgcaccaa gtacaccgtc acgatcgacg ccgccctgcg tgacgcctac ggccagaccc
     1081 tcggcaagcc cgagcagcgc agcttcaccg tcggccccgc ccccgacagc ctgcacgcgc
     1141 tcgtccagca gctgaccgtg ctcgacccct ccgcgcccgg caaactctcg gtgttcacga
     1201 ccggccacga gagcctcaag gtccagatcc acgccgtcac cccggccgac tgggacgctt
     1261 acttaaaatt cctgcgcgcg cgcaacgagg acacgctgcg acgcgcgacc ccgccgggca
     1321 agcctgtgtt ctccgacacg gtcgagatca aaggcgaccc cgacgtcctc accgagaccg
     1381 cgatcgacct gcgcgccgcg ctgcccgcgg gcctcggtca cgcgatcgtc gtcgtcacgc
     1441 cgaccaaggt ccccaaggag ccgtggatgc ggcacgaggt gatcacctgg gcccaggcga
     1501 cgcgcatcgg cctcgacgcc ttcgtcgaca acgagaagct cgtcggcttc gccacccgcc
     1561 tcgccgacgg cgccgccatc ccgaacgtgc agctgcaact gctgcccagc ggcgccaggg
     1621 cgacgaccgg cgccgacggc ctcgccagcc tgccgctgcc gcaaaagggc aaccgcgtgc
     1681 tgctggccac ccagggccag gaccgggcgt ttttgccgga gcaggtcggc tggtggtacg
     1741 gcgaggacga gggcggctgg cgcccgtaca agcgtgagga cagcctgcac tggtacgtct
     1801 tcgacgaccg ccagatgtat cgccccggcg agaccgcgaa gatcaagggc tggctgcgca
     1861 gccgcacccc cggcctgcgc gggggcctcc agctcccgac cagcgccgtc aaggatctgc
     1921 gctggaccct gcacgacagc cagggcaaca agctgctgac cggcaccacc aaggtcaacg
     1981 cgctcggcgg cttcgacctc tcgctgaccc tgccgaggac ccccaacctc ggccacgccg
     2041 gcctgacctt cgaggtcacc gccgggacca tgaagggcca gagctgggcc cacggcctcc
     2101 agatccagga gtttcgccgg cccgagtacg aggtcaaggc gcgcgccagc gagggcccgc
     2161 acctgatcgg cagccacgcc gacctcaccg tctcggcgca gtacttcgcc ggtgggccgc
     2221 tcgccaacgc cgaggtcgcc tggcaggtca gcgccagctc cggcagctac agcccgcccg
     2281 gccacgacga ctacaccttt ggccgctgga ccccgtggtg gggctggggc atgcgcgaga
     2341 tcggcttccc gggccgcgag tatcacccgc ccgagagctt cgccggtcgc accgatgcat
     2401 cgggcgagca ccacctgcgc gccgacttcc tctcggtcga cccactcgag gcgacgagcc
     2461 tgcacgccga ggcgaccgtg ctcgacgtca accgccaggc ctggacctcc ggcgtcgacc
     2521 tcctggtcca ccccgcgagc ctctacgtcg gcatgaagag cgaccgcatg ttcgtccagc
     2581 agggcgagcc cctgcacatc gacgcgatcg tcaccgacat cgacggcaag caggtccagg
     2641 gccaggacat cctgatccgc gccgtgcgga tcgcctggga gcaggtcggc ggcgagtaca
     2701 aggagaccga aaaagacccg caggactgca agcaagcctc ggccgcggcc gcagtgcgct
     2761 gcagcttcat cccgaaggag ggcggg
//
LOCUS       DASWNJ010001583         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_664577, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001583 DASWNJ010000000
VERSION     DASWNJ010001583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664577"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1441
                     /locus_tag="VGB85_26290"
     CDS             <1..1441
                     /locus_tag="VGB85_26290"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEY0137633.1"
                     /translation="RTAHVAGLVDEPLQIHALRGTFVPGKDGLKVQMSGIGLDEIELT
                     EGTIDVVRCAAGSCPYKADGYAVYLGGVQNNTPERVPGAALRARIGDRGRMSMWGHAV
                     LSPDFERVSVSNVRAHLDEIDYRMYDNSGRPQLYATIASDDVVLEGRDSMTLRGEVLV
                     NRGRFVRDAQEGVKVLSFAQASTAPAAPPPEIVRDMALDLRIRTVAPFRVDNNVMKGV
                     EGQVLLAIGGTLGDVEMSGKVDVTTGVLDVAILNGAYDIQYGKVQLERDLAASTVDVL
                     ALRQNPIYVENQPRQMYVKLGGTLDAITWRCIVQGDTRTRGRTTKECVDYLVLGAGNK
                     EVADANVRRYGGGGLLGRPIGLVGTLTELKLGKYVEKNAPRIAPYVPDVGMRLGQLGI
                     EVNAETPRPWFRSDWGNLTVGAGYTRGYPGLLLRNSYDWRVRFRFLDSASLEFRDNRR
                     SYFNERIIFDPLRQRSLELRLDYQLPSLR"
     gene            1488..>2006
                     /locus_tag="VGB85_26295"
     CDS             1488..>2006
                     /locus_tag="VGB85_26295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137634.1"
                     /translation="MIATAAMILGGPAPARAAQNLSLGPGYSGHVLAAMQTPVADPKS
                     PGDPDPAGPPAPAPPSAPTKPPTTGKKSGGGPATSPTTSPTTGPAGGPTTAPKNAPTP
                     DPDTAPADPADGAAANELPPSLTNTDDPQAPRDDNVAPATQVEAPPDIAAAAADAAAE
                     AKAGGADEGPLAG"
BASE COUNT          308 a          843 c          627 g          228 t
ORIGIN      
        1 gcgcaccgcc cacgtcgccg gcctcgtcga cgagccgctg cagatccacg ccctgcgcgg
       61 caccttcgtg cccggcaagg acggcctcaa ggtccagatg tcggggatcg gcctcgacga
      121 gatcgagctc accgagggca cgatcgacgt cgtccgctgc gccgcgggca gctgcccgta
      181 caaggccgac ggctacgccg tctacctcgg cggcgtccag aacaacaccc ccgagcgcgt
      241 ccccggggcc gccctgcgcg cccgcatcgg cgaccgcggg cgcatgagca tgtggggcca
      301 cgccgtgctc agccccgact tcgagcgcgt cagcgtcagc aacgtgcgcg cccacctcga
      361 cgagatcgac tatcgcatgt acgacaactc gggccggccg cagctgtacg cgacgatcgc
      421 cagcgacgac gtcgtgctcg agggccgcga cagcatgacc ctgcgcggcg aggtcctggt
      481 caaccgcggc cggtttgtcc gcgacgcgca ggagggcgtc aaggtgctat cgttcgcgca
      541 ggcgagcacc gcccccgccg cgcccccgcc agagatcgtc cgcgacatgg ccctcgacct
      601 ccggatccgc accgtcgcgc cgtttcgcgt cgacaacaac gtcatgaagg gcgtcgaggg
      661 ccaggtcctg ctggcgatcg gcggcacgct gggcgacgtc gagatgtccg gcaaggtcga
      721 cgtcaccacc ggcgtgctcg acgtcgcgat cctcaacggc gcctacgaca tccagtacgg
      781 caaggtccag ctcgagcgcg acctcgcggc cagcaccgtc gacgtgctcg cgctgcgcca
      841 gaacccgatc tacgtcgaga accagccgcg ccagatgtac gtcaagctcg gcggcaccct
      901 cgacgcgatc acctggcgct gcatcgtcca gggcgacacg cggacccgcg gccgcacgac
      961 caaggagtgc gtcgactacc tcgtgctcgg cgccggcaac aaggaggtcg ccgacgccaa
     1021 cgtgcgccgc tacggcggcg gcggcctgct cggccgcccg atcggcctcg tcggcaccct
     1081 gaccgagctc aagctcggca agtacgtcga gaagaacgcc ccgcgcatcg cgccgtacgt
     1141 gcccgacgtc ggcatgcgcc tcggccagct cggcatcgag gtcaacgccg agaccccgcg
     1201 cccatggttc cgcagcgact ggggtaacct gaccgtcggc gccggctaca cccgcggcta
     1261 ccccggcctg ctcctgcgca acagctacga ctggcgcgtc cgcttccgct tcctcgactc
     1321 cgcctcgctc gagttccgcg acaaccgccg ctcctacttc aacgagcgca tcatcttcga
     1381 ccccctccgc cagcgatcgc tcgaactgcg ccttgactac cagctcccca gcctgcgcta
     1441 gccgcccgcc gcgtcgcggc agccgcctgt tcgccgacct cgccgctgtg atcgccacgg
     1501 cggcgatgat cctcggcggc ccggccccgg cacgcgccgc gcagaacctg tccctcgggc
     1561 caggttattc gggacatgtc ctcgcggcca tgcagacgcc ggtcgcggac cccaagtcac
     1621 ccggcgaccc cgaccccgcc ggaccgcccg cccccgcccc gccctccgcg ccgaccaagc
     1681 caccgacgac cggcaagaag tccggcggcg gcccggccac ctccccgact acctccccga
     1741 ccaccgggcc cgccggcggc ccgaccaccg cgccgaagaa cgccccgacg cccgaccccg
     1801 acaccgcccc ggccgacccc gccgacgggg ccgccgccaa cgagctcccg ccctcgctga
     1861 ccaacaccga cgacccgcag gccccgcgcg acgacaacgt cgcgcccgcg acccaggtcg
     1921 aggccccgcc cgacatcgcg gccgcggccg ccgacgcggc cgcagaggcc aaggccggcg
     1981 gcgccgacga gggcccgctc gccggc
//
LOCUS       DASWNJ010001584         2242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_132597, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001584 DASWNJ010000000
VERSION     DASWNJ010001584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132597"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2242
                     /locus_tag="VGB85_26300"
     CDS             <1..>2242
                     /locus_tag="VGB85_26300"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEY0137635.1"
                     /translation="AAATLQTFDAQSTQYQATLATSNSKTTWTRPLATAHDHMQRVID
                     YYAKTHARNSWDGKGAQVRQLVHFGQDFPNAFWDSYNKHMAIGDGDATYFKEFTRALD
                     VSAHEFSHAVVTGTVDLVYQGQPGALNESFADVMSVMVDRDDWLIGEDIVRPGQFPKG
                     YARSLSDPAGGEQPKHMSGLYKGFDDFGGVHTNSGIPNHAAYLLATAKSRELVEKIWY
                     RTLYKNHVGSQASFVDMAEGTLTACDELVAAKQASAGDCTATAEAWVAVGVLGKADVP
                     MEGCPQNASEKGGVCYCDPGYVPNGDGSTCVAVENVVCPANSIEANGQCFCKDGFKPS
                     ADSSQCVAVDQGCPLDSSWDALAKACKCDPGFEGAPNAADGKCEAIDSDCPTHAHPEW
                     PDANMPDQYLCACNQNYQDDGQGGCEVVPGTCGSESFFGRCDGTTLTYCDVSGEPTIS
                     VVDCKADGLVCGKFDSIVGYDCLNPGGVAAGGECEADGYQQCDDTVPFCVSEADASDG
                     FCSHECKAKSECAGTQVEKDAFDCCATVSDGTRACLKDPYCAENIDTKATCDDVPGGS
                     SYFGKCDGDVLVYCDGSTDTTQEVFCNTLGLECRWVDKATGYSCVEPNSGALPEAPDD
                     WCPYENDGTCDAPGKCPEGTDLLDCNPCGEVLEAGVCNGDTLAVCDPQVGLVTTKCAE
                     MGMVCDPNNSGGATCVPGDGEDTGEPTPTEGGGDTATQNGDADSGGDGGQDDGGGETI
                     SCTCRGD"
BASE COUNT          406 a          751 c          808 g          277 t
ORIGIN      
        1 gccgcggcga ccctgcagac cttcgacgcc cagagcacgc agtaccaggc gacgctggcg
       61 acctccaaca gcaagacgac ctggaccagg ccgctggcga ccgcgcacga ccacatgcag
      121 cgggtgatcg actactacgc caagacccac gcgcgcaact cgtgggacgg caagggcgcg
      181 caggtgcggc agctggtgca cttcgggcag gacttcccga acgcgttctg ggactcgtac
      241 aacaagcaca tggcgatcgg cgacggcgac gcgacgtact tcaaggagtt cacgcgcgcg
      301 ctcgacgtct cggcccacga gttctcgcac gcggtcgtca ccggcaccgt cgacctcgtg
      361 tatcagggcc agccgggcgc gctcaacgag agcttcgccg acgtcatgtc ggtgatggtt
      421 gaccgcgacg actggctgat cggcgaggac atcgtccggc cggggcagtt ccccaagggc
      481 tacgcgcgca gcctctccga ccccgcgggc ggcgaacagc ccaagcacat gagcgggctg
      541 tacaagggct tcgacgactt cggcggggtc cacaccaaca gcgggatccc caaccacgcg
      601 gcgtacctgc tggcgaccgc gaagagccgc gagctggtcg agaagatctg gtatcgcacg
      661 ctctataaga atcatgtcgg cagccaggcg agcttcgtcg acatggccga gggcacgctg
      721 accgcctgcg acgagctcgt cgcggccaag caggcgagcg ccggcgactg caccgcgacc
      781 gcggaggcgt gggtcgccgt cggcgtgctc ggcaaggccg acgtgccgat ggagggctgc
      841 ccgcagaacg ccagcgagaa gggcggcgtg tgctactgcg accccggcta cgtgcccaac
      901 ggcgacggct cgacctgcgt ggcggtcgag aacgtggtgt gcccggccaa cagcatcgag
      961 gccaacggcc aatgtttctg taaagacggc ttcaagccga gcgcggacag cagtcaatgc
     1021 gtcgcggtcg accagggctg tccgctcgac agcagctggg acgcgctcgc caaggcgtgc
     1081 aagtgcgacc cgggcttcga gggcgccccg aacgcggcgg acggcaagtg cgaggcgatc
     1141 gactcggact gcccgaccca cgcgcacccc gagtggcccg acgcgaacat gccggaccag
     1201 tacctgtgcg cgtgcaacca gaactaccag gacgacggcc agggcggctg cgaggtcgtg
     1261 cccgggacct gcggcagcga gtcgttcttc ggccgctgtg acggcaccac cctcacctac
     1321 tgcgacgtct ccggcgagcc gacgatctcc gtcgtcgact gcaaggccga cggcctggtg
     1381 tgcggcaagt tcgacagcat cgtcggctac gactgcctca accccggcgg cgtcgcggcc
     1441 ggcggcgagt gtgaagcgga cggctatcaa cagtgcgacg acacggtgcc gttctgcgtg
     1501 tcggaggcgg acgcgagcga cggcttctgc tcgcacgagt gcaaggcgaa gtcggagtgc
     1561 gcgggcacgc aggtcgagaa ggacgcgttc gactgctgcg cgacggtgtc cgacggcacg
     1621 cgcgcgtgcc tcaaggaccc gtactgcgcc gagaacatcg acaccaaggc gacctgcgac
     1681 gacgtgccgg gcggcagctc gtacttcggc aagtgcgacg gcgacgtgct ggtgtactgc
     1741 gacggctcga ccgacacgac gcaggaggtg ttctgcaaca cgctcggcct cgagtgccgc
     1801 tgggtcgaca aggcgaccgg ctacagctgc gtcgagccga attcgggggc gttgccggag
     1861 gcgccggacg actggtgtcc gtacgagaac gacggcacgt gcgacgcgcc gggcaagtgc
     1921 ccggagggca ccgacctgct cgactgcaac ccgtgcgggg aggtgctcga agccggcgtg
     1981 tgcaacggcg atacgctcgc ggtctgcgac ccgcaggtcg gcctggtcac cacaaaatgc
     2041 gccgagatgg gcatggtctg cgacccgaac aactcgggcg gggcgacgtg cgtgccgggc
     2101 gacggcgagg acaccggcga gccgaccccg accgagggcg gcggcgacac ggcgacgcag
     2161 aacggcgacg cggacagcgg cggcgacggc ggccaggacg acggcggcgg cgagacgatc
     2221 agctgcacgt gccgcggcga cg
//
LOCUS       DASWNJ010001585         2273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_450094, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001585 DASWNJ010000000
VERSION     DASWNJ010001585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450094"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2273)
                     /locus_tag="VGB85_26305"
     CDS             complement(<1..>2273)
                     /locus_tag="VGB85_26305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137636.1"
                     /translation="LDSLSFSERLRAAARIARAHADDPALPARIDAWLAAGAHEGLLA
                     VVMAQTIRDRPRLVAAIGHRSLLVRQAAIEATLRIEAVPEDSRTLPALVVALPPALRR
                     ALLKAIVRSGRRSVGQALFAEVLERHGPRDAAVLLPVLEDSTLRTVLPSLAHAVGSWR
                     SLYLRHPTVTVEHLQTLLAAAAPRDQAGVWQSHNALIGALAELRGAALFDLLDRFGDT
                     CPVHLLWSRLGELARADSERLVTLLERPRLRDMALRAGLPRRLLNQTVRFTPAQRVRL
                     ARVVGERPELLAPLLAVVAPSQREALLAAATADQERTSRVWPDVLLDSLPRALRRHEA
                     ARMITLPQVRADPDRVQALSAYLDLARASEVLAPALRAAAAELRGEAIARLIACAGRE
                     GAMTTTLAQLAPRLRNDQDPVRLKAFTALARVPPTAFVPADVDPLQAMVVAATEARDT
                     SHATRQQIQQIAFRLLRTHAAAAGDALFQAGLDILVRLAGQTQTLMLPDLSKGLPRRT
                     APEIVAALAPRIAAANAREQHDLVLGLATALGRRAYGLHALTALLEPLQHSKPDHLAT
                     RAITLLLADPRTRDERVRALIAWDPSVIALHPVLMHLHRRRQPWLDPYLTGTPVKGRF
                     LTGKTIYVLALQSGFHRWLPRQQQAFAHVLARSAGDTGQPQLGRAKVIARLARMPITG
                     VADFAPYLQSKDVTVIEAALGALVWIDTPADALPVLLAHLDSDRARVAMYALPRVARF
                     IDGATLAGALLELVAGP"
BASE COUNT          299 a          790 c          920 g          264 t
ORIGIN      
        1 cgggcccggc gacgagctcg agcagcgcgc cggccaaggt ggccccgtcg atgaagcgtg
       61 cgaccctcgg gagggcgtac atcgcgacgc gcgcgcggtc gctgtcgagg tgagccagca
      121 ataccggtag tgcgtcggcg ggcgtgtcga tccagacgag cgcgccgagg gccgcctcga
      181 tcactgtgac gtccttgctc tgcagatacg gcgcgaagtc ggcgacgccg gtgatcggca
      241 tgcgcgcgag gcgggcgatc accttggcgc ggccgagctg cggctggccc gtgtcgccgg
      301 ccgagcgcgc gagcacgtgc gcgaacgcct gctgttgccg cggtagccag cggtggaagc
      361 cggactgcag cgcgagcaca tagatcgtct tgccggtgag aaagcggccc ttgaccggcg
      421 tgccggtcag gtacgggtcg agccatggct gccggcgccg gtgcaggtgc atcagcaccg
      481 gatgcaacgc gatcacgctc gggtcccacg cgatcagggc ccgcacgcgc tcgtcgcggg
      541 tccgcgggtc ggcgagcagc agggtgatcg cgcgcgtcgc gaggtggtcg ggcttgctgt
      601 gctgcagcgg ctccagcagc gcggtcaggg cgtgcaggcc gtacgcgcgg cggccgagcg
      661 cggtcgccag gccgagcacc aggtcatgct gctcgcgcgc gttggcggcg gcgatcctcg
      721 gcgccagcgc ggcgacgatc tcgggcgcgg tgcgccgcgg cagccccttc gagaggtcgg
      781 gcaacatcag ggtctgggtc tgcccggcga gccggacgag gatgtcgagg ccggcctgga
      841 acagcgcgtc gccggccgcg gccgcgtgcg tgcgcagcag acgaaacgcg atctgctgga
      901 tctgctggcg ggtcgcgtgg ctggtgtcgc gcgcctcggt cgcggcgacg accatcgcct
      961 gcagcggatc gacgtcggct ggcacgaagg cggtcggcgg cacgcgggcc agcgcggtga
     1021 aggccttgag gcgcacgggg tcctggtcgt tgcgcaggcg cggcgcgagc tgggcgaggg
     1081 tcgtcgtcat cgcgccctcg cggccggcgc aggcgatcag gcgggcgatc gcctcgccgc
     1141 gcagctcggc ggcggcggcc cgcaacgcgg gcgccagcac ctcgctcgcg cgcgcgaggt
     1201 cgaggtaggc cgacagcgcc tgcacgcgat ccggatcggc gcgcacctgc gggagcgtga
     1261 tcatgcgggc cgcctcgtgg cgtcgcagcg cccgcggcaa gctgtccaaa agcacatccg
     1321 gccagacccg cgaggtccgc tcctggtccg cggtcgcggc ggccagcagc gcctcgcgct
     1381 gcgacggggc gacgaccgcg agcagcggcg cgagcagctc gggccgctcg ccgacgacgc
     1441 gggcgaggcg gacgcgctgg gccggcgtga agcggacggt ctgattgagc aggcggcgcg
     1501 gcaggccggc gcgcagcgcc atgtctcgca ggcgcgggcg ctcgagcagc gtcacgagcc
     1561 gctcgctgtc ggcgcgcgcg agctcgccga gccgcgacca cagcaggtgc acggggcagg
     1621 tgtcgccgaa gcgatcgagc aggtcgaaga gggcggcgcc gcgcagctcc gcgagcgcgc
     1681 cgatcaaggc gttgtggctc tgccacaccc ccgcctggtc gcgcggcgcc gcggcggcga
     1741 gcagggtctg caggtgctcg acggtcaccg tcgggtggcg caggtacagg ctgcgccagc
     1801 tgccgaccgc gtgcgcgagc gaaggcagta cggtgcgcag cgtgctgtcc tcgaggacag
     1861 gtaaaagcac cgccgcgtcg cgcggcccgt ggcgctcgag cacctcggcg aacagcgcct
     1921 ggccgacgct gcgccgcccg ctgcggacga tcgccttgag cagcgcgcgg cgcagcgccg
     1981 gcggcagggc gacgaccagc gcgggcaggg tccggctgtc ctccgggaca gcttcgatcc
     2041 gcagcgtcgc ctcgatcgcc gcctggcgga ccagcagcga gcggtgaccg atcgccgcga
     2101 cgagccgcgg acggtcgcgg atcgtctggg ccatcaccac cgcgagcagg ccctcgtgag
     2161 cgccggccgc cagccaggcg tcgatgcggg cgggcagggc cgggtcgtcc gcgtgcgcgc
     2221 gggcgatcct cgcggccgcg cgcaggcgtt cggagaacga cagcgagtcg agc
//
LOCUS       DASWNJ010001586         2292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_311628, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001586 DASWNJ010000000
VERSION     DASWNJ010001586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311628"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VGB85_26310"
     CDS             <1..529
                     /locus_tag="VGB85_26310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY0137637.1"
                     /translation="RGDVLLEHAPVDHLHAVIAKLEEAGARCIVEPRGLRVIGPDELQ
                     PIDIVTRAHPGFPTDMQAQMMVLATCAHGQSILTETIFENRFMHVPELVRLGADIHVN
                     GNVAIIKGPAKYMGTTVMATDLRASASLVLAGLVASGQTEIRRVYHLDRGYETIEKRL
                     RKVGAKIRRFHGAQS"
     gene            513..1208
                     /gene="hisG"
                     /locus_tag="VGB85_26315"
     CDS             513..1208
                     /gene="hisG"
                     /locus_tag="VGB85_26315"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEY0137638.1"
                     /translation="MAPRADGLTLALPKGRILAQAIPLLAQAGLDLASVLSGEHDRRL
                     LLDLPGGLRVLLVKPVDVPTYVEHGVADLGVAGYDTLCEEARDLYQPVDLGIGACRLV
                     VAEPAARPVALRRGMALRVATKYPRLALRHYRSRGIQPEIIPLAGSVELGPLTGLSDQ
                     IVDLVESGETLRQNNLRETDTILHVTSRLIVHPASLKLKQSAIASVISAVRAAVAASA
                     VSSVVSSVVSRTA"
     gene            1205..1912
                     /locus_tag="VGB85_26320"
     CDS             1205..1912
                     /locus_tag="VGB85_26320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137639.1"
                     /translation="MSKHDDPALVRTALLQRLKVHTRVAQQGMGPTPDVRKKLASSLP
                     DILAFGDRRYAAAHVVLDWDHRLPSEALLLRLYLSYTDREAADVENALKARDREIDSG
                     NLYPEFDVPDYGEIDASETYVGLVRPKGQEVEELRFFSDWRKSVQQPVVRDVLAAVRG
                     HAGYERSMRERSHDNLGPPVVIGWTPPCLANSKHWAVEVWLLVDFDGHMGRAHVFMVD
                     SKLRNVTREFFTEVQIG"
     gene            1925..>2292
                     /locus_tag="VGB85_26325"
     CDS             1925..>2292
                     /locus_tag="VGB85_26325"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY0137640.1"
                     /translation="MPEAPRERTSRDDLVSALLRVGLIALLLLLAWWLVDVLLLTFAG
                     VLIAVFLRAPADWLASRTKLSGNLSIAIVFVVLTLVLGGAGFAMAPEIGRQVDELTQR
                     VPEAARELTGSLQQYGWGRWL"
BASE COUNT          351 a          836 c          744 g          361 t
ORIGIN      
        1 ccgcggggac gtgctgctcg agcacgcccc cgtcgaccac ctgcacgccg tgatcgccaa
       61 gctcgaagag gccggggcgc gctgcatcgt cgagccgcgg ggcctgcggg tgatcggccc
      121 cgatgaattg cagccgatcg acatcgtcac gcgcgcgcac ccgggcttcc cgaccgacat
      181 gcaggcgcag atgatggtgc tcgcgacctg cgcgcacggt caaagcatcc tgaccgagac
      241 gatcttcgag aaccgcttca tgcacgtgcc ggagctggtg cgcctcggcg cggacatcca
      301 cgtcaacggc aacgtggcga tcatcaaggg cccggcgaag tacatgggca cgaccgtgat
      361 ggcgaccgac ctgcgcgcga gcgccagcct ggtgctcgcc ggcctggtcg ccagcggcca
      421 gacggagatc cgccgcgtct atcacctcga ccgcggctac gagaccatcg agaagcgcct
      481 ccgcaaggtc ggcgcgaaga tccgcaggtt ccatggcgcc cagagctgac ggcctcaccc
      541 tcgccctgcc aaaaggcagg atcctggccc aggcgatccc gctgctcgcc caggccggac
      601 tcgacctcgc ctcggtcctg tccggcgagc acgaccgccg cctcctcctg gacctcccgg
      661 gcggcctgcg cgtgcttttg gtcaagcccg tcgacgtccc cacctacgtc gagcacgggg
      721 tcgcagacct cggcgtcgcc ggctacgata cgctgtgcga ggaagcacgc gacctctacc
      781 agccggtcga cctcgggatc ggcgcgtgcc ggctggtggt cgccgagccg gcggccagac
      841 cggtcgcttt acgccgtggc atggccctga gggtcgccac gaagtatccg cgcctcgccc
      901 tgcgccacta ccgctcgcgc gggatccagc cggagatcat cccgctggca ggttcggtcg
      961 agttgggccc gctcacagga ctgtccgacc agatcgtcga tcttgtggag agcggcgaga
     1021 cgctgcggca aaataacctc cgggagaccg acaccattct tcacgtcacc agccggctca
     1081 tcgttcaccc cgcgagcctc aagctcaagc agtcggcgat cgcctccgtg atctccgcgg
     1141 tgcgggcggc cgtcgctgcc tctgccgttt cttccgtcgt ttcttccgtc gtttcaagga
     1201 ctgcatgagc aaacacgacg acccggccct cgtccgcacg gccctcctcc agcggctcaa
     1261 ggtccacacc cgtgtggcac agcagggcat gggcccgaca ccggacgtgc gcaagaagct
     1321 tgcttcgtca cttccggaca tcctcgcctt tggtgaccgc cgctacgccg cggcgcacgt
     1381 ggtgctcgac tgggaccacc gcctgccgag cgaggcgctg ctgctgcgcc tctacctcag
     1441 ttacaccgac cgcgaggcgg ccgacgtcga gaacgccctc aaggcccgcg accgcgagat
     1501 cgactccggc aacctctacc cggagttcga cgtccccgac tacggggaga tcgacgccag
     1561 cgagacctac gtcggcctcg tgcgacccaa gggccaggag gtcgaggagc tgcgcttttt
     1621 cagcgactgg cgaaagagcg tccagcagcc ggtcgtgcgc gatgtgctcg cggccgtgcg
     1681 cggccacgcc ggctacgagc gctccatgcg cgagcgcagc cacgacaacc tcggaccccc
     1741 ggtcgtcatc ggctggaccc cgccctgcct cgccaacagc aagcactggg cggtcgaagt
     1801 ttggctgctc gtcgacttcg acggccacat gggccgggcc cacgtgttca tggtcgacag
     1861 taaactgcgt aacgtgaccc gtgagttctt cacggaggtt cagatcggct agataaacgg
     1921 ggccatgccc gaggccccgc gcgagcgtac atcccgtgac gacctggtca gcgccctcct
     1981 gcgggtcggc ctgatcgcgc tgctgctgct gctggcctgg tggctggtcg atgtcctgct
     2041 gctgaccttc gccggcgtgc tgatcgcggt gttcctgcgg gcccccgccg actggctcgc
     2101 cagccgcacc aagctgtcgg gcaacctctc gatcgcgatc gtgttcgtgg tgctgacgct
     2161 cgtgctcggc ggggccggct tcgcgatggc gccggagatc ggccgccagg tcgacgagct
     2221 gacccagcgg gtccccgagg ccgcgcgcga gctgaccggc tcgctccagc agtacgggtg
     2281 gggccgctgg ct
//
LOCUS       DASWNJ010001587         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_311639, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001587 DASWNJ010000000
VERSION     DASWNJ010001587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311639"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(396..>1509)
                     /gene="rlmN"
                     /locus_tag="VGB85_26330"
     CDS             complement(396..>1509)
                     /gene="rlmN"
                     /locus_tag="VGB85_26330"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY0137641.1"
                     /translation="ARGIPRSGPQNLRDLDLAGLTSWLAQQSPSSPRHRADQLFRWLH
                     QRRATSFKDMSDLPAAERARLGGLASLDRLTVDAVFTARDGTRKLRLRTADGAALESV
                     LIPNEDRGFTQCVSSQVGCALTCRFCATASLGFQRNLATWEIADQVYQAQDLLAREAA
                     AEGAEYPGRITNIVFMGMGEPLHNYNQVKRAIHLLTHAHGAAIAGRRITISSSGLVPA
                     IERFARDELSRDVGLAISLNATTDAVRDQVMPINTRWNIATLLAAVRQVPQHRRRYVT
                     FEYVLLADVNDTDDDARRLSALVGDMRCQVNVIPFNPHPHAPYRRPSASRVQRFMSRC
                     RALGLHVYLRTPRGDDIGAACGQLALEAGAVPVADA"
BASE COUNT          208 a          479 c          586 g          236 t
ORIGIN      
        1 tccgataaat ccgcggggca tccccggacc cccgagcatg cgaccccgtg gaacatgcgc
       61 gcctggcctc gcttgtctgg ctgtctctcc ggacaggtga cgatcgggac cgactgtcgg
      121 tcttcgaccg cccgtcggtc ccgcgtgttc ggacgcgatg ttcgccagac gcgtgccgtg
      181 gtgaggcacc gtgtcgaggg tcgcatgcct gggcgcatcg ttgcccgtcc ggacgggagc
      241 gctgcactgc ccgagcgctt tgccgcgcgc gttcggtcgg ggatggggca acggcgtaag
      301 cgctttgcgg cgctgatggg gacgcggcat gcggcgtcag gcgtccgcga cggtacggct
      361 ttgcgcgcgt gcggccgcac tgggacgcgg cgggcctagg cgtcggcgac cgggacggcg
      421 ccggcttcga gggcgagctg gccacaggcg gcgccgatgt cgtcgccacg cggggttcgc
      481 agatacacgt gcaggccgag ggcgcggcag cggctcatga agcgttgcac gcggctcgcg
      541 gagggccggc gatatggggc gtgcgggtgc gggttgaacg ggatgacgtt gacctggcag
      601 cgcatgtcgc cgaccagcgc cgagagtcgg cgggcgtcgt cgtcggtgtc gttgacgtcg
      661 gcgagcagga cgtactcgaa ggttacgtag cggcgccggt gctgcgggac ctggcggacc
      721 gcggcgagca gggtggcgat gttccagcgg gtgttgatcg gcatcacctg gtcgcgcacg
      781 gcgtcggtcg tcgcgttgag gctgatcgca aggccgacat cgcggctgag ctcgtcgcgc
      841 gcgaagcgtt cgatcgcggg gaccaggccc gagctcgaga tcgtgatccg gcgaccggct
      901 atcgcggccc cgtgggcgtg ggtcagaagg tggatcgccc gcttgacctg gttgtagttg
      961 tgcaggggct cgcccatgcc catgaacacg atgttggtga tccggcccgg gtactcggcg
     1021 ccctcggccg cggcctcgcg ggcgagcagg tcctgggcct ggtacacctg atccgcgatc
     1081 tcccaggttg cgaggttgcg ctgaaagccg aggctggcgg tcgcgcagaa ccgacacgtc
     1141 agcgcgcagc cgacctgcga ggacacacac tgcgtgaagc cgcgatcctc gttggggatc
     1201 aacacgctct cgagcgcggc accatcggcg gtgcgcagcc gcagcttgcg ggtgccgtca
     1261 cgcgcggtga acacggcgtc gaccgtgagc cgatcgagcg aggcgagacc gccgaggcga
     1321 gcgcgctccg ccgccggcag gtcggacatg tctttaaaag aggtcgcgcg gcgctggtgc
     1381 agccagcgaa acagctggtc ggcacggtgc cgcggactgc tcggactctg ctgcgcgagc
     1441 cagctcgtca aacccgcgag atcgaggtct cgcaggttct gtgggccgct gcgcgggatg
     1501 ccccgcgcg
//
LOCUS       DASWNJ010001588         2573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_450139, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001588 DASWNJ010000000
VERSION     DASWNJ010001588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450139"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1877
                     /locus_tag="VGB85_26335"
     CDS             <1..1877
                     /locus_tag="VGB85_26335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137642.1"
                     /translation="SGLDPMLPWQIIRIDAGPLGPIDVNLGPGGAFVVERTADAIEIR
                     SDPFLEIRAERQDFVPILAFRKIRWEPATDAWSVEFDPPVGPLIPEAMQRVAHALAGP
                     ILAKVAELAALPAPPSGPLPPAAPPPVAGDIVFEKDVPKIGPVRVAADPNHVVDVVFN
                     RGAAEITLGSGVVARMPGLGFNVQVNGVRAAMHPIGAHLDTKPDAGPLFDHVIEHTLR
                     TLLHQHADKFWPTDEAVRIGQDTLLVLGRGQAWGPLRVCVPTDGDIRVHLDTQGFALR
                     SEGGIFISGQAIDWLPDFYLHTLGYAFEDGAVTLEISGIEETYYHEAHPVSPVTQALL
                     AHLIRVLALPKLPAWTAKLGLRSFPLPPVPTGDPARISMYRLKLPGDFGEVQISMPPN
                     DTVTVRIDDNEASVISQHGLFATLPGLRFELQLRGIRYHMHSGEIQVGGLGQLENALL
                     EAVVARQLRNVPQIAKETPAEVTSALSTLLDQLPMDDHGRRVLFSHTLVNLLLPENPG
                     LIVRFTSNGLAFTSDPPIVIDGPARIDFAFNGIRYSFAHASFHLDLDNAGAAVSGMFT
                     PIIINQAEKRLNNMFKPLLPAPMREPGYNLATDPRSEEHILDIIEGFALLGKKKKATS
                     "
     gene            complement(2035..2475)
                     /locus_tag="VGB85_26340"
     CDS             complement(2035..2475)
                     /locus_tag="VGB85_26340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137643.1"
                     /translation="MSHKIQGARKQAVGGRSSPIKWRACGSQLRDRVGFVDGVETLQI
                     THRGSGYTLSFHHEEKARPATLEEARARAELDLEQWREERRLRREQEAAATARQLRDR
                     RAREERLARLIADLNAAGVEAREVFGAITLTDAAAEGLLARISG"
BASE COUNT          390 a         1032 c          768 g          383 t
ORIGIN      
        1 agtccgggct cgacccgatg ctgccgtggc agatcatccg catcgacgcc ggcccgctcg
       61 gcccgatcga cgtcaacctc ggccccggcg gcgccttcgt cgtcgagcgc accgccgacg
      121 cgatcgagat ccgctccgac ccgttcctcg agatccgggc cgagcgccag gacttcgtgc
      181 ctatcctggc gtttcgcaag atccgctggg agcccgcgac cgacgcgtgg agcgtcgagt
      241 tcgacccgcc cgtcggcccg ctgatcccgg aggccatgca gcgggtcgcg cacgcgctcg
      301 ccggcccgat cctcgccaag gtcgccgagc tcgccgcatt gcccgcgccg ccctccggcc
      361 cgctgccgcc cgctgcccca ccgccggtcg ccggcgacat cgtcttcgag aaggacgtgc
      421 ccaagatcgg ccccgtccgg gtcgccgccg accccaacca cgtcgtcgac gtcgtgttca
      481 accgcggcgc cgccgagatc acgctcggca gcggcgtggt cgcccgcatg ccgggcctcg
      541 gcttcaacgt ccaggtcaac ggcgtacgcg ccgcgatgca cccgatcggc gcccacctcg
      601 acaccaagcc cgacgccggc ccgctgttcg accacgtcat cgagcacacc ctgcgcaccc
      661 tcctgcacca gcacgccgac aagttctggc cgaccgacga ggccgtgcgg atcggtcagg
      721 acaccctgct cgtgctcggc cgcggccagg cctggggccc cctgcgcgtg tgcgtgccga
      781 ccgacggcga catccgcgtc cacctcgaca cgcagggttt cgcgctgcgc tccgagggcg
      841 gcatcttcat ctccggccag gcgatcgact ggctgccgga cttctacctg cacaccctcg
      901 gctacgcctt cgaggacggc gccgtcaccc tcgagatcag cggcatcgag gagacctact
      961 atcacgaggc gcaccccgtc agccccgtca cgcaggcgct gctcgcccac ctgatcaggg
     1021 tgctcgcgct gcccaagctg cccgcgtgga ccgccaagct cggcctgcgc agcttcccgc
     1081 tcccgccggt cccgaccggc gacccggccc ggatctcgat gtatcgcctc aagctgcccg
     1141 gcgacttcgg cgaggtccag atctcgatgc cgcccaacga caccgtcacc gtgcgcatcg
     1201 acgacaacga ggcgtccgtg atcagccagc acggcctgtt cgccaccctg cccggcctgc
     1261 gcttcgagct ccagctgcgc ggcatccgct atcacatgca cagcggcgag atccaggtcg
     1321 gcggcctcgg ccagctggag aacgcgctgc tcgaggccgt cgtcgcccgc cagctgcgca
     1381 atgtccccca gatcgccaag gagacccccg ccgaggtcac ctccgcgctg agcaccctgc
     1441 tcgaccagct gccgatggac gaccatggcc gccgggtcct gttctcgcac acgctcgtca
     1501 acctgctgct gccggagaac ccgggcctga tcgttcgttt cacctcgaac ggcctcgcct
     1561 tcacctccga cccgccgatc gtcatcgacg gcccggcccg catcgacttc gccttcaacg
     1621 gcatccgcta cagcttcgcc cacgcgagct tccacctcga cctcgacaac gccggcgccg
     1681 ccgtctccgg catgttcacc ccgatcatca tcaaccaggc cgagaagcgc ctcaacaaca
     1741 tgttcaagcc gctgctcccc gccccgatgc gcgagcccgg ctacaacctc gccaccgacc
     1801 cccgctccga ggagcacatc ctcgacatca tcgagggctt cgccctgctc ggcaaaaaga
     1861 agaaagccac gagctgaggc gcgaagcctc gggaataaaa agcgccgccg tgccccgcac
     1921 ggcggcgttt ttgcgtcggt ggacccggct gatcggcgat tgctactttg ccgtcgaagc
     1981 gggccgaacc gccagcgtgc ccctgcttcg tcgggtcgag cgcacgagcc ggcgtcaccc
     2041 cgagatccgg gccaggagtc cctcggcggc cgcatcggtc aatgtaatcg ccccgaagac
     2101 ctcccgggcc tcgacgcccg ccgcgttgag atcggcgatc agccgggcga gtcgttcttc
     2161 ccgggcccgc cggtcgcgca gttgccgggc cgtcgcggcg gcctcctgct cgcgtcggag
     2221 ccgtcgctcc tctcgccatt gctcgaggtc cagctcggca cgggccctcg cctcctcgag
     2281 cgtggcgggc cgcgccttct cctcgtgatg aaagctcagt gtgtagcccg acccgcggtg
     2341 cgtgatctgc agcgtctcga cgccgtcgac gaagccgacg cgatcccgca gctgcgaacc
     2401 gcaggccctc cacttgatcg gtgagctgcg gcctcccacg gcctgcttgc gggctccctg
     2461 tatcttgtga gacatttaaa gacatcatac gagagacagt gacaggacgc aagacaacag
     2521 ccccgcggct agactccgcg cgtgccgggc ccccccaagg tcacgacgaa gct
//
LOCUS       DASWNJ010001589         7918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_98937, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001589 DASWNJ010000000
VERSION     DASWNJ010001589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7918
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98937"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1449
                     /locus_tag="VGB85_26345"
     CDS             <1..1449
                     /locus_tag="VGB85_26345"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY0137644.1"
                     /translation="TVPADGSAQARQLTSGLANNFTPAWSPDGKAIVFSRSRTGPRDA
                     HRTDLWRMNADGSDARQLTDGLAVVSAPAWSPDGSKIAFQGSEREGDARMGLWVIDAE
                     GGAPRRLGGDDFEIASYPLDNSSPPYWTSDSSALFVLQGHQSTSRIVRITLADEAVTP
                     IVDDDRQVTMLSVAPAADRLIFAASDTLRFCELFTVTLAGGEPRRLAAPNAAWAAGRL
                     EYEVEHRTFRSSDGEGEGRDGWLLMPRGDGGPRPLWVDFHGGPHTFVSLGYPYHPYWH
                     VLLSQGWAVLALNTLGSASYGASFAERLRGSWGESDLPEHLAAIDQLVEEGVVDPKRI
                     AIGGKSYGGYLSAWAIGQTQRFCAAVVSAPVADMVSHFGTSDSGYYVTPFAMDGEVHE
                     KRETYDRLSPVRWLDNVTTPTLLLQGEEDQRCPVGQSEEIFATLMRLGKAPVEFVRYP
                     GGDHHLAEQGTPSHRVDYTRRIVGWAQRWSSQ"
     gene            1555..2055
                     /locus_tag="VGB85_26350"
     CDS             1555..2055
                     /locus_tag="VGB85_26350"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY0137645.1"
                     /translation="MARPPRISRPLVAMRLALRQRVIYADTDQMGVVYHGTYPRFLEA
                     ARVEFMRALGHTYATVEAHGLGMPLTDLGIAYMAPARYDDIVSVHVGVVELSFARLRF
                     GYHLTVEPGDRVGLAAPLTVLFAETCHCCIVLADGRPTRFPDELRATLLPLATPSPPA
                     PTAPLG"
     gene            2070..3041
                     /gene="glpX"
                     /locus_tag="VGB85_26355"
     CDS             2070..3041
                     /gene="glpX"
                     /locus_tag="VGB85_26355"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEY0137646.1"
                     /translation="MDRTLVLEYTRVTEAAAISAATQIGRGDGMSADAAAVKAMRAAF
                     ERVPARGTIVIGEGERDEAPMLFIGEKVGPNDPRLLEVDIAVDPLEGTNLCANGLPGS
                     LAVLAVAHKGQLLNAPDTYMSKIACGPEGKGKISLEKSPRDHLWALAAAKRRRPDELT
                     VVVLDRPRHESLIQELRAAGARILLITDGDVAPVVATCIPKSGIDMVYGIGGAPEGVL
                     GAAAVHCMGGEFQGRLQFRNDHERERAIAMGMKDPDQILHVNDLARGDVLFVATGVTT
                     GPLLKGVRRIGTRMHLQSLALRSTTGTIRWVNTDVDAHRYIHEYDHA"
     gene            3050..3970
                     /locus_tag="VGB85_26360"
     CDS             3050..3970
                     /locus_tag="VGB85_26360"
                     /inference="COORDINATES: protein motif:HMM:NF040941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-like YCDxxxxGGGW domain-containing
                     protein"
                     /protein_id="HEY0137647.1"
                     /translation="MHRSLALALLCLAGCPGEVIEGTGAPATTATTTGTTDTPDPSTT
                     DTPTTSPNDPSTSTGPMTASTTGTTGTAPTSTGPDTTTGAPCGNGVPDPGEQCDDGNE
                     LQGDDCTNACTRAASCLELLGLDATLPDGEYTIDPDGDGTAVMVYCDMAGGGWTRVVD
                     EDLDTPMGWAGGASSQCGGIGSLLGGAGQLGAGATLSKGFALLGVPHTQLRASAAVAI
                     IDTWDSETINLEVDAEIVASKLCVYVDTASCNQVDQQCGEPTAGDGEIVLAGELMHAT
                     DAALVKLSTTLNEAASNEAWGLNSLQVYVR"
     gene            3992..6280
                     /locus_tag="VGB85_26365"
     CDS             3992..6280
                     /locus_tag="VGB85_26365"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0137648.1"
                     /translation="MRPMRPMLPRARLAVLAACLAAGGLALLRAGPPAPRDSDAPLHE
                     FSALRARERLATLLADGAPHPTGTAANAAVRARLVEQLRALGLSPRELPAFSCTDYGN
                     CGPVVNVLVEVPGTTPGPALLLASHFDSVPAGAGAADDGHGVVVVLEALRALLADGPP
                     RTPLMAVFTDGEESGLLGARAFVEDPAFVAVGAVINIEARGSGGAARMFETSDGNAAL
                     ISAYATGAPRPSAQSLSYEVYRRLPNDTDLTIFKKAGAQGLGFAFIGDVRHYHTPLDD
                     LDHLDLGSVQQQGDAVLGTARQLLAAGIQPPAGNATYVDLLGMFVLRWPAALDLPLAG
                     LALLTLLFAAWRAIRGRISTDVPSDRSRQISPRALAAAMGSALLAPVVGAVASYAVLS
                     LAGALAGPIGTWPAALMLSLAAGAAAASALAVLVLRGVARRVGARAQALGTWIVWALL
                     ACPVAVLLPGASILWIAPAVLAGAGLLLAGHSPRLRAFACALAGALAWCIWAPLLPAL
                     TEALGLDALVLGALAGWTWTAASPALADAPDQRHAARLSLGLGVLALALAVAAAVAPR
                     VTPELPAKLNFFELTDLSTGRTRHVLDTLMNIDGLPAEFMADPWRTDIPLPWSGRTMY
                     VSPPFDGPADGPRYVQTSSTSNGDLRRISGTLTPRPGALVMLVVIPGDALTSLTVGGR
                     TLDVATLQRGHGDTRVMRIHGPPAAGLEIVAELRGEAPWVLADMLPGLTDASAELVAR
                     RPVDRVPYQTGDLRIAFRSFKP"
     gene            complement(6284..7135)
                     /locus_tag="VGB85_26370"
     CDS             complement(6284..7135)
                     /locus_tag="VGB85_26370"
                     /inference="COORDINATES: protein motif:HMM:NF019274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor
                     domain-containing protein"
                     /protein_id="HEY0137649.1"
                     /translation="MSRSTRTAWSVCLFAATFALAAPGCDDPASDGGIDDGEVAQPNL
                     TPPHDATPTDASAAPETCDCPKVGSPVCGSDGGTYANACKAACADVEVQHDGPCIPVQ
                     CANDEACGEGEFCDFGDSCGGVGTCAPQPELCYRKLAPVCGCDGETYENSCLAHAVGV
                     SVAAKGECAGECVCTLEYAPVCGTDGQTYGNACQAGCADVEVDHAGECVDSCDHNGQC
                     GTGRFCQRDASCGGGGTCVDQPQVCADLYQPVCGCDGKTYGNECEAHAAGISFETQGA
                     CGGIDVE"
     gene            7529..>7918
                     /locus_tag="VGB85_26375"
     CDS             7529..>7918
                     /locus_tag="VGB85_26375"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0137650.1"
                     /translation="MSAIRMATPSDIPRLQEIRAAVRENVLRNPGLVTLADYHAHIDG
                     RGRTWVAEHHGHIVGFASADRETASIWALFVDPAHAGRGHGRRLLDQAVAWLWDCGAE
                     RIVLSTEPGSRAESVYRAAGWIVTGTST"
BASE COUNT         1076 a         3029 c         2689 g         1124 t
ORIGIN      
        1 acggtgccgg ccgacggcag cgcgcaggcc cggcagttga cctccgggct cgccaacaat
       61 ttcaccccgg cctggtcgcc cgacggcaag gcgatcgtgt tctcgcgctc gcgcaccggc
      121 ccgcgcgacg cccatcgcac ggacctgtgg cgcatgaacg cggacggcag cgacgcgcgc
      181 cagctgaccg acggcctcgc cgtcgtcagc gcgccggcgt ggtcgcccga cggcagcaag
      241 atcgcgttcc aggggtccga gcgcgagggc gacgcgcgca tgggcctgtg ggtgatcgac
      301 gccgagggcg gcgcgccgcg gcggctcggc ggcgacgact tcgagatcgc cagctacccg
      361 ctcgacaaca gctccccgcc gtactggacc agcgacagct ccgcgctgtt cgtcctgcaa
      421 ggccatcaaa gcaccagcag gatcgtccgc atcacgctgg ccgacgaggc ggtcacgccg
      481 atcgtcgacg acgaccgcca ggtcacgatg ctgagcgtcg cgcccgcggc cgaccggctg
      541 atcttcgcgg cgagcgacac cctgcgcttt tgtgagctct tcaccgtgac gctcgccggc
      601 ggcgagccgc gtcgcctggc ggcccccaac gccgcgtggg cggccggtcg cctcgagtat
      661 gaggtcgagc accgcacgtt tcgcagcagc gacggcgagg gcgagggacg cgacggctgg
      721 ctgctgatgc ccaggggcga cggggggccg cggccgctgt gggtcgactt ccacggcggc
      781 ccacacacct tcgtgtcgct cgggtacccc tatcatccgt actggcacgt gctgctgtcg
      841 cagggctggg ccgtgctggc gctcaacacg ctcggctcgg cctcgtacgg tgcgagcttc
      901 gcggagcgcc tgcgcgggag ctggggcgag tcggacctgc cggagcacct cgccgcgatc
      961 gaccagctgg tcgaggaggg cgtggtcgac cccaagcgga tcgcgatcgg cggcaagtcg
     1021 tatggcggct acctgtcggc gtgggcgatc ggtcagacgc agcgcttctg cgccgcggtc
     1081 gtgtcggccc cggtcgcgga catggtctcg cactttggca catccgacag cggctactac
     1141 gtgaccccgt tcgcgatgga cggtgaggtc cacgagaagc gcgagaccta cgaccgcctc
     1201 tcgccggtgc gctggctcga caacgtgacc acgccgaccc tgctgttgca gggcgaggag
     1261 gaccagcgct gcccggtcgg gcaatccgag gagatcttcg ctacgctgat gcgcctcggc
     1321 aaggcgcccg tcgagttcgt gcgctacccc ggcggggatc atcacctcgc ggagcagggc
     1381 acgccctcgc atcgcgtcga ctacacgcgc cggatcgtcg gctgggcgca gcgctggtcg
     1441 agccaatagg cattcgcgtc gtcgcgaggc cagcgctgac cgcggcctaa ctcactgcga
     1501 cgatcgatcc atcatgcgcg cgcgcccccg ggctccggta gcctcgcggg cgcgatggct
     1561 cgcccgcccc ggatctcccg ccccctcgtc gccatgcgac tcgcgctgcg ccagcgggtg
     1621 atctacgccg acaccgacca gatgggggtc gtgtaccacg gcacctaccc tcgcttcctc
     1681 gaggccgcgc gcgtcgaatt catgcgcgcc ctcggccaca cctacgcgac cgtcgaggcc
     1741 cacggcctcg gcatgccgct gaccgacctc ggcatcgcgt acatggctcc ggcgcgctac
     1801 gacgacatcg tctcggtgca cgtcggcgtc gtcgagctga gcttcgcgcg cctgcgcttc
     1861 gggtatcacc tgaccgtcga gccaggtgat cgcgtaggtc tcgcggcgcc gctgacggtg
     1921 ctgttcgccg agacctgcca ctgctgcatc gtcctcgcgg acggtcggcc gacccgcttc
     1981 cccgacgagc tgcgagcgac gctgctgccg ctcgcgaccc catcgccccc cgccccgacc
     2041 gccccgctgg gctgacacag gagacctgca tggatcgcac actagttctg gagtacacgc
     2101 gcgtgaccga ggccgcggcg atctccgccg cgacgcagat cggtcgcggc gacgggatgt
     2161 cggccgacgc cgccgccgtc aaggcgatgc gcgccgcgtt cgagcgcgtg ccggcccgcg
     2221 gcacgatcgt catcggcgag ggcgagcgcg acgaggcgcc gatgttgttc atcggcgaga
     2281 aggtcggccc caacgacccg cgcctgctcg aggtcgacat cgccgtcgac ccgctcgagg
     2341 gcacgaacct gtgcgcgaac ggcctgccgg gctcgctcgc ggtgctcgcg gtggcccaca
     2401 agggccagct gctcaacgcc cccgacacgt acatgagcaa gatcgcctgc ggcccggagg
     2461 gcaagggcaa gatctcgctc gaaaaatcgc cgcgcgacca cctctgggcg ctcgccgccg
     2521 ccaagcgccg ccggcccgac gagctgacgg tcgtcgtgct cgaccgaccg cgccacgaga
     2581 gcctcatcca ggagctgcga gccgccggcg cgcgcatcct gctgatcacc gacggcgacg
     2641 tcgcgcccgt ggtcgccacc tgcatcccca agagcggcat cgacatggtc tacgggatcg
     2701 gcggggcgcc cgagggcgtg ctcggggccg ccgcggtgca ctgcatgggc ggcgagttcc
     2761 agggccgcct gcagtttcgc aacgaccacg agcgcgagcg tgcgatcgcc atgggcatga
     2821 aggaccccga ccagatcttg cacgtcaacg acctcgcgcg cggcgacgtg ctgttcgtcg
     2881 ccaccggcgt gaccaccggg ccgctgctca agggcgtgcg ccggatcggc acccgcatgc
     2941 acctgcagag cctcgcgctg cgcagcacca ccggcacgat ccggtgggtc aacaccgacg
     3001 tcgacgcgca ccgctacatc cacgagtacg atcacgccta ggagtccgca tgcaccgctc
     3061 gctcgccctc gcattgttgt gcctcgcagg ttgccccggc gaggtgatcg agggtaccgg
     3121 cgcccctgcc acgacggcga ccacgacggg caccacggac acaccggacc cgagcacgac
     3181 ggacacgccg accacctcgc cgaatgaccc ctcgacgagc accggcccca tgaccgccag
     3241 caccacgggc accacgggca ctgccccgac ctcgacgggc ccggacacga ccaccggggc
     3301 gccctgcggc aacggcgtcc ccgaccctgg cgagcagtgt gacgacggca acgagctgca
     3361 gggcgacgac tgcaccaatg cgtgcacgcg cgccgcgagc tgcctcgagc tgctcgggct
     3421 cgacgcgacc ttgcccgacg gcgagtacac gatcgacccc gacggcgacg gcaccgcggt
     3481 catggtgtac tgcgacatgg ctggtggtgg ctggacgcgg gtcgtcgacg aggacctcga
     3541 caccccgatg ggctgggccg gtggggcgag ctcgcagtgc ggcggcatcg gcagcctgct
     3601 cggcggcgcc ggccagctcg gggccggcgc gacgctctcc aagggcttcg cgctgctcgg
     3661 cgtcccgcac acgcagctgc gcgccagcgc ggcggtggcg atcatcgaca catgggacag
     3721 cgagacgatc aacctcgagg tcgacgccga gatcgtcgcc agcaagctct gcgtgtatgt
     3781 cgacaccgcc tcctgcaatc aggtggacca gcaatgtggt gagccgaccg ccggtgacgg
     3841 cgagatcgtc ctcgccggcg agctgatgca cgcgaccgac gcagcgctcg tcaagctgag
     3901 caccaccctc aacgaggccg cgagcaacga ggcctggggt ctcaacagct tgcaggtgta
     3961 cgtccgctag cgcggcgcgt accatcatcc catgcgcccc atgcgcccga tgctcccgcg
     4021 cgcccgtctc gccgtgctcg ccgcttgcct cgccgccggc ggcctggcgc tgctgcgcgc
     4081 cggtccgccc gcgccgcgcg acagcgacgc gccgctgcac gaattctccg cgctgcgggc
     4141 ccgcgagcgc ctcgccacgt tgctcgccga cggcgccccg cacccgaccg gcaccgccgc
     4201 caacgcggcc gtgcgcgccc gcctggtcga gcagttgcgc gcgctcggcc tgagcccgcg
     4261 cgagctgccc gcgttctcgt gcacggacta tggcaactgc ggcccggtgg tcaacgtgct
     4321 cgtcgaggtc cccggcacca cccccggccc ggcgctgctg ctcgcctcgc acttcgattc
     4381 ggtgcccgcc ggcgcgggcg cggccgacga cggccacggc gtcgtcgtcg tgctcgaggc
     4441 gctgcgcgcc ctgctggccg acggcccgcc gcgcacgccc ctgatggccg tgttcaccga
     4501 tggtgaggag tccggcctgc tcggcgcgcg cgccttcgtc gaggacccgg ccttcgtcgc
     4561 cgtcggtgcc gtcatcaaca tcgaggcgcg gggcagcggc ggcgcggcgc gcatgttcga
     4621 gaccagcgac ggcaacgcgg ctttgatctc cgcgtacgcg accggggccc cgcgaccgag
     4681 cgcccagtcg ctgtcgtacg aggtctaccg ccgcctgccc aacgacaccg acctgaccat
     4741 ttttaaaaag gccggcgcgc aggggctcgg cttcgcgttc atcggcgacg tccgccacta
     4801 tcacaccccc ctcgacgacc tcgaccacct cgacctcggc agcgtgcaac agcagggcga
     4861 cgccgtgctc ggcacggccc gccagctgct cgccgccggt atccagccgc ccgcgggcaa
     4921 cgcgacctac gtcgatctgc tcggcatgtt cgtgctgcgc tggccggccg cgctcgacct
     4981 gccgctcgcc ggcctcgccc tgctgacgct gctgttcgcc gcgtggcggg cgatccgcgg
     5041 gcgtatctcc acagatgtcc cgtcggacag gtcacgacag atctcgccgc gggcgctcgc
     5101 cgccgccatg ggctcggcct tgctcgcccc tgtggtcggc gcggtcgcct cgtacgcggt
     5161 gttgagcctc gcgggtgcgc tcgccgggcc gatcgggacc tggccggccg cgctgatgct
     5221 cagcctcgcc gcgggcgcgg cggctgccag cgcgctcgcg gtgctcgtat tgcgcggcgt
     5281 ggcgcggcgc gtcggggcgc gggcccaggc gctcggcacg tggatcgtgt gggccctgct
     5341 cgcctgcccc gtcgcggtct tgctgccagg cgcgagcatc ctgtggatcg cgccggccgt
     5401 gctcgcgggc gccggattac tcctcgccgg tcactcgcct cgcctgcgcg cgttcgcctg
     5461 cgccctcgcc ggcgccctcg cgtggtgcat ctgggcgccg ctgttgcccg cgttgaccga
     5521 ggcgctcggc ctcgacgcgc tcgtgctcgg ggccctcgcc ggctggacct ggaccgccgc
     5581 ctcccccgcc ctcgccgacg cgccggacca gcgccacgcg gcgcgcctct cgctcggcct
     5641 cggcgtcctc gcgctcgcgc tcgccgtcgc cgcggccgtc gccccgcgcg tcacccccga
     5701 gctgccggcc aagctcaact tcttcgagct gaccgacctc tccaccggtc gcacgcgcca
     5761 cgtgctcgac accctgatga acatcgacgg cttgccggcc gaattcatgg ccgacccgtg
     5821 gcgcaccgat atcccgctgc cgtggagcgg tcgaacgatg tatgtatcgc cgcccttcga
     5881 cggccccgcg gacggcccgc gatatgtaca gaccagcagc acctccaacg gtgatctgcg
     5941 ccggatctcc ggcacgctta cgccccgccc cggcgcgctg gtgatgctcg tcgtcatccc
     6001 cggcgacgcg ctgacctcgc tgacggtcgg cggccgcacg ctcgacgtcg ccacgctaca
     6061 acgcggccac ggagacaccc gcgtcatgcg gatccacggc ccgcccgcgg cgggcctgga
     6121 gatcgtcgcc gaactgcgcg gcgaggcccc gtgggtgctc gccgacatgc tgcccgggct
     6181 caccgacgcg tccgcggaat tggtcgcgcg acgcccggtg gaccgcgtcc cgtatcagac
     6241 cggcgacctg cgcatcgcct ttcggtcctt taaaccatga gcctcactcg acgtcgatcc
     6301 cgccgcacgc gccctgggtc tcgaagctga tgccggcggc gtgcgcctcg cattcgtttc
     6361 cgtaggtctt gccgtcgcag ccgcacaccg gctggtacag gtcggcgcat acctgcggct
     6421 ggtcgacgca cgtaccaccg ccgccgcagc tcgcgtcgcg ctggcagaag cgacccgtgc
     6481 cgcactggcc gttgtggtcg cagctgtcga cgcactcgcc cgcgtggtcg acctcgacgt
     6541 ccgcgcagcc ggcctggcac gcgttgccgt aggtctggcc gtcggtgccg cacacgggcg
     6601 cgtactccag cgtgcacacg cactcgccgg cgcactcgcc cttggccgcg acgctgacgc
     6661 cgaccgcgtg cgcgaggcag ctgttttcat acgtctcgcc gtcgcagccg cacaccgggg
     6721 cgagcttgcg gtaacaaagt tcgggctgcg gcgcgcaggt gccgacgccg ccgcagctgt
     6781 cgccgaagtc gcagaactcg ccctcgccgc aggcctcgtc gttggcgcac tggaccggga
     6841 tgcagggccc gtcgtgctgg acctcgacat ccgcgcaggc ggccttgcag gcgttcgcat
     6901 aggtcccgcc gtcggaccca cagacggggc tgccgacctt ggggcagtca cacgtctccg
     6961 gcgcagcgct cgcatccgtg ggcgtcgcgt cgtgcggcgg ggtgaggttc ggctgcgcga
     7021 cctcgccatc gtcgatgcca ccatcgctgg cgggatcgtc acaaccgggg gccgcgagcg
     7081 cgaacgtggc cgcgaacaga catacggacc acgccgttcg tgtggagcga gacatggcaa
     7141 acctgcttta caacgtgaaa tggtgagagg tcggccgggg caggccatcg cacggacctc
     7201 gcgcgacacc atgtctgtgg cacgcatcgc cgacccctgc cacccgttgt ttgcgcgcag
     7261 aacccggcga attggcggtt atacgagaca attcgcggtt tgtgacagtc gggggagaac
     7321 cgggctttcc tgtcaatgcc gggaatgtcg gcacatgaaa gcaccggcct cacgacgcag
     7381 tcgccgcggg gccgtcgcgc acccgaaccg gcgcgagcac ctcgggcgtc acctcgagca
     7441 tacattcgac gtcacgccgc agccgcgtca gcgcccgatc cccgggccgg gctttgctcg
     7501 cgcgccgccc cgcgatatcg tgccgaacat gtccgcgatc cgcatggcga ccccgtccga
     7561 catcccgcgc ctgcaggaga tccgcgccgc cgtccgcgag aacgtgctgc gtaaccccgg
     7621 cctggtgacc ctggccgact atcacgcgca tatcgacggc cgcggccgca cctgggtcgc
     7681 cgagcaccac ggccacatcg tcggcttcgc ctccgccgac cgcgagaccg cgtcgatctg
     7741 ggcgctgttc gtcgaccccg cgcacgccgg tcgcggccac ggccgccgcc tgctcgacca
     7801 ggccgtcgcg tggctgtggg actgcggggc cgagcgcatc gtgctgtcga ccgagccggg
     7861 ctcgcgcgcc gagtcggtgt atcgcgcggc aggatggatc gtcaccggca cgagcacg
//
LOCUS       DASWNJ010001590         2971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_410964, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001590 DASWNJ010000000
VERSION     DASWNJ010001590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410964"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VGB85_26380"
     CDS             complement(<1..488)
                     /locus_tag="VGB85_26380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137651.1"
                     /translation="MSKIRELAGAARAASHALAALPGPARSALLRDLAAALRDPDTRA
                     PVLAANAADLAAAAEAHARGELDPARYKRLSLDAAKLDGVAHGLEQLSEAPELLGRPD
                     LRRELDEDLVLERVPCPLGVLGVVFEARPDAVPQIAGLALKSGNAAVLKGGREALQSN
                     RAL"
     gene            complement(531..1151)
                     /locus_tag="VGB85_26385"
     CDS             complement(531..1151)
                     /locus_tag="VGB85_26385"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY0137652.1"
                     /translation="MSIRRTLSLALVASLALACKRDVSERPGSPETVEPAPASAETPA
                     KRSSRKPPKAEPAASSSKESGRFAGMTAAHNRVRARLKIAPLEWSPELARFAQKWADK
                     LARKGCDLQHRPRTGPDAQRYGENIFAISGAPATADDVVDAWAAEVKFYNAKANRCKG
                     VCGHYTQVVWRNSERVGCGMATCGDDTEVWVCNYDPRGNYNGERPY"
     gene            complement(1195..1704)
                     /locus_tag="VGB85_26390"
     CDS             complement(1195..1704)
                     /locus_tag="VGB85_26390"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEY0137653.1"
                     /translation="MTDILAAVVSIAPTRRRRFLWCAWWTESPSRDPFRKPDASSGGA
                     RTREEALREAEAAAGRSLVEVEGLWARAWARVLMGQPAFPPGASGAPAGAAETRARRD
                     PPAGSIWQVLGVDTGATVEQIRAAFRQRVLVTHPDHGGDPDAFRQLRAAYDEALERRA
                     RARKRPRRK"
     gene            complement(1921..2133)
                     /locus_tag="VGB85_26395"
     CDS             complement(1921..2133)
                     /locus_tag="VGB85_26395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137654.1"
                     /translation="MAKKPEKITLNHDFAFASDADLNAQIAAFTAAHEAEHRASTGER
                     LAMDARRSLGPSKVRVTFRIVPGKKR"
     gene            2181..>2971
                     /gene="tgt"
                     /locus_tag="VGB85_26400"
     CDS             2181..>2971
                     /gene="tgt"
                     /locus_tag="VGB85_26400"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEY0137655.1"
                     /translation="MPLPFRVAAVDENSRARRGVLETAHGPVDTPAFMSVGTRASVTG
                     LTPTDLREVGAQVVLGNTYHLMLRPGVELFRRVGGIHRFMRWDGPILTDSGGYQIFSL
                     SEDRIISEKGATFRSYTNQQKHMLSPERSIEVQTAIGSDIMMVLDVCVDARSDLPTTR
                     AAMERTHRWALRSLAARSNPQQALFAIMQGGIFPELRRESAAFLTGHPFDGFAIGGLA
                     VGDTRAEREEVTHMAAELLPAERPRYLMGVGTPPDLLHAMLAGVD"
BASE COUNT          398 a         1003 c         1125 g          445 t
ORIGIN      
        1 agggcgcggt tgctctgcaa cgcttcgcgg ccgcccttga gcaccgccgc gttgccgctc
       61 ttgagcgcga gccctgcgat ctgcgggacg gcgtcggggc gggcctcgaa cacgacgccg
      121 agcacgccga gcgggcaggg cacacgctcg agcacgaggt cctcgtcgag ctcgcggcgc
      181 aggtcggggc ggccgagcag ctccggggcc tcggacaatt gctcgaggcc gtgggcgacg
      241 ccgtcgagct tggcggcgtc gagcgagagc cgcttgtaac gggcaggatc gagctcgccg
      301 cgcgcgtggg cctcggcggc ggcggcgagg tccgccgcgt tggcggcgag gaccggcgcg
      361 cgggtgtccg ggtcgcgcag ggcggcggcg aggtcacgca gcagggcgct gcgggccggg
      421 ccgggcagcg cggcgagggc gtggctggcg gcccgggcgg cgccggcgag ttcgcggatc
      481 ttcgacattg cgagattgtc ggggaagcgt cgcgggttgg cgactgcttt tcagtagggg
      541 cgctcgccgt tgtagttgcc ccgcgggtcg tagttgcaga cccacacctc ggtgtcgtcg
      601 ccgcaggtcg ccatgccgca gccgacgcgt tcgctgttgc gccacacgac ctgcgtgtaa
      661 tgcccgcaca cgcccttgca gcggttggcc ttcgcgttat agaacttgac ctcggcggcc
      721 caggcgtcga cgacgtcgtc cgcggtcgcg ggggcgccgg agatggcgaa gatgttctcg
      781 ccgtagcgct gcgcgtccgg gccggtgcgc gggcgatgtt gcaggtcgca gcccttgcgc
      841 gcgagcttgt cggcccactt ctgggcgaag cgtgccagct cgggcgacca ctcgagcggc
      901 gcgatcttca ggcgagcgcg cacgcggttg tgggcggcgg tcatgcccgc gaagcggccg
      961 gattcttttg aagaggaggc ggcgggctcg gccttcggcg gcttgcggga cgagcgcttg
     1021 gcgggtgttt cggcgctggc cggcgcgggc tcgacggtct cgggggaacc tggcctttcg
     1081 gacacgtcac gcttgcaggc gagggcgagc gaggcgacga gcgcgagtga gagggtgcga
     1141 cggatcgaca cgcggaccag catgaccgct gcgcggtgga tcgccaagtc cgcgtcactt
     1201 gcgacgcggg cgcttcctcg cgcgggcgcg gcgctcgagg gcctcgtcgt aggcggcgcg
     1261 cagctggcga aacgcgtcgg ggtcaccgcc gtggtcgggg tgggtgacga gcacgcgctg
     1321 gcgaaacgcg gcccggatct gctcgacggt cgcgccggtg tcgacgccga ggacctgcca
     1381 gatcgacccg gcgggcggat cgcggcgcgc acgggtctcc gcggcgcccg ccggcgcacc
     1441 actggcgccc ggcgggaacg cgggctgacc catcaatacg cgggcccacg cgcgcgccca
     1501 caggccctcg acctcgacga gcgagcgacc cgcggcggcc tcggcctcgc gcagcgcctc
     1561 ctcgcgcgtg cgagccccgc cgctcgaggc gtcgggtttt ctaaaagggt cgcgcgacgg
     1621 cgactcggtc caccacgcgc accacaggaa gcgccgccgg cgggtcggcg cgatcgatac
     1681 gacggcggcg aggatgtccg tcacgggccc ggcacgatac cgcgtggcaa cagatctgcc
     1741 agcgctcgtt ccgtattgcg gatagttttc gtatatagcg tcacctgata aaaaggcggc
     1801 gagatcgtga ggcacgcgag gggccggcgg gatcaccgga gagtgtcgcg cggcccggac
     1861 ggtggccatg cgccgtctca cgtgaccgag ggccggggtt tcccggcacc tgcgaggaaa
     1921 tcagcgcttc ttgccgggca cgatgcggaa ggtgacgcgg accttgctcg gcccgagcga
     1981 gcgccgggcg tccatcgcca gccgctcgcc ggtgctcgcc cggtgctccg cctcgtgggc
     2041 cgcggtgaag gcggcgatct gggcgttgag gtcggcgtcg ctggcaaacg cgaagtcgtg
     2101 attcagggtg atcttctccg gcttcttcgc catgcgcgcg gacgctacca cgacgcgcga
     2161 ggggttacac tccggccccc atgccgctcc cgttccgcgt cgccgccgtc gatgagaatt
     2221 cacgcgcgcg tcgcggcgtg ctcgagacag cccacggccc ggtcgacaca cccgcattca
     2281 tgagcgtcgg cacgcgcgcg tcggtcacgg ggctgacacc cacggacctg cgcgaggtcg
     2341 gggcgcaggt ggtgctcggc aatacctatc acctgatgct gcgccccggc gtcgagctgt
     2401 ttcgccgcgt cggtgggatc caccgcttca tgcgctggga cggcccgatc ctgaccgact
     2461 ccggtggcta tcagatcttc tcgctgtcgg aggaccggat catcagcgaa aagggcgcga
     2521 cctttcgcag ttataccaat cagcaaaagc acatgctctc gcccgagcga tcgatcgagg
     2581 tgcagacggc gatcgggtcc gacatcatga tggtgctcga cgtctgcgtc gacgcgcgct
     2641 ccgacctgcc gacgacccgc gcggcgatgg agcgcaccca tcgctgggcg ctgcgcagct
     2701 tggccgcgcg cagcaacccc cagcaggcgc tgtttgcgat catgcagggc ggcatcttcc
     2761 ccgagctgcg ccgcgagtcg gcggcgttct tgaccgggca tcccttcgac ggctttgcga
     2821 tcggtggcct cgcggtcggt gacacccggg ccgagcgcga ggaggtcacg cacatggccg
     2881 ccgagctgct gccggccgag cgcccgcgat atctcatggg ggtcggcacg ccgccggacc
     2941 tgctgcacgc gatgctcgcg ggcgtcgata t
//
LOCUS       DASWNJ010001591         3788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_64291, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001591 DASWNJ010000000
VERSION     DASWNJ010001591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64291"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VGB85_26405"
     CDS             complement(<1..831)
                     /locus_tag="VGB85_26405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137656.1"
                     /translation="MQPSRGVIAGLSVGLLGAGLLAPIELWSLATDDPQVVWACLSLL
                     GGLGVAIGATIAISEWLVARLRPRPLRAAAIRAASCLPVLIPLAQHLFEGAFAQTLPG
                     ASLAPVFVPLLGWLALTLTFWPGSRLLQYPRPESGLAPLRAGALPRFIVRRRLMALAA
                     TTSALAVEYVNRRFLRGVYPDIHTTALVISCVGVGLGVWLGLTRPWLYDSRVERRLAV
                     AQSRAIVLALLISIDLAVTANLAACLVLGLPTHDARLAISERGLHTRLLVRVARGLVD
                     Q"
     gene            complement(1092..2870)
                     /gene="thiC"
                     /locus_tag="VGB85_26410"
     CDS             complement(1092..2870)
                     /gene="thiC"
                     /locus_tag="VGB85_26410"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEY0137657.1"
                     /translation="MDPRNTDTHPSLAAEATPPQAVDSPLTPAPPRPGSLPLAAPEVT
                     PVRAIAPLVESFPNSAKVTEGELAVPFRRISQSGGNPDIVVYDTTGPQGIDPRQGLPP
                     RRKAWIEARVSSADGNLSQMHYARRGVITEEMRFCAIRERMDPEFVRSEVALGRAIIP
                     ANIKHPELEPMIIGRNFLVKINANIGNSAVRSSIEEEVEKLQWATRWGADTVMDLSTG
                     KQIHTTREWILRNAPVPIGTVPIYQALEKVKGKAEDLNIDVFMETLHEQAEQGVDYFT
                     IHAGVLLRYIPLTAKRVTGIVSRGGSIMAKWCLHHHKENFLYTEFERICALMQKYDIS
                     FSLGDGLRPGCIADANDEAQFGELRTLGELTQVAWKHDVQVMIEGPGHVPMHKIKENM
                     DEQLRLCHEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGSFGTAMLCYVTPKEHLGLP
                     DRQDVKDGVIAYKIAAHAADLAKGHPGAQDRDDALSKARFEFRWHDQFNLSLDPETAK
                     DFHDETLPAEPAKEAHFCSMCGPHFCSMKLTQDVRDMAELAAVSAAAEEGMAAKAAEF
                     RAAGGELYVPKDRHGSAAEALEGAAE"
     regulatory      complement(2861..2957)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3309..>3788
                     /locus_tag="VGB85_26415"
     CDS             3309..>3788
                     /locus_tag="VGB85_26415"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0137658.1"
                     /translation="MQMQAQLRTLAGALALVLACGGEDGPGSATEAGSSSSSGEVVTT
                     TGDMVTTTGDIPASCGDGKVDPGEGCDDGDANGEAAECTPECQLQVCGDGHVGAKEGC
                     DDGNQNDDDDCTNACALSTCGDGVVLQGAEECDDGDDDNGDNCLKNCKLAVCGDGYVR
                     "
BASE COUNT          595 a         1222 c         1427 g          544 t
ORIGIN      
        1 ctggtcgacg aggccacgcg cgacgcggac cagcagccgt gtgtgcaggc cgcgctcgct
       61 gatcgccagg cgagcgtcgt gggtcggcag gccgagcacg aggcaggccg cgaggttggc
      121 ggtcaccgcg aggtcgatcg agatcagcag cgccagcacg atcgcccgcg actgggccac
      181 agcgaggcgc cgctcgaccc gcgagtcgta cagccacggg cgcgtcaggc cgagccacac
      241 gccgaggccg acgccgacgc aggagatcac gagcgcggtg gtgtggatgt cggggtagac
      301 gccgcgcaaa aacctgcggt tgacgtactc gacggcgagc gccgaggtgg tcgcggcgag
      361 ggccatcagg cgccggcgga cgatgaagcg cggcagggca ccagcgcgca gcggggccag
      421 gccgctctcg gggcgcgggt actgcagcag gcggctacct ggccaaaagg tcagggtgag
      481 cgcgagccag ccgagcagcg gcacgaacac gggcgcgagg ctggcgccgg gcagggtctg
      541 ggcgaaggcc ccctcgaaga ggtgctgggc cagcgggatc agcacgggca ggcacgaggc
      601 ggcccggatc gcggcggccc gcagcggacg cggacgcagg cgagcgacca gccactcgct
      661 gatcgcgatc gtcgcgccga tcgcgacccc gaggccgccg agcagcgaca ggcaggccca
      721 cacgacctgc gggtcgtcgg tggcgagcga ccacagctcg atcggcgcga gcaggccggc
      781 gccgagcaga ccgaccgaga ggccggcgat cacgccgcgc gaaggctgca cacgacgcga
      841 ccgcagaccg ggggccaggc cgggctggtc gggcgcggtc gcgcgaatgt cggcgtccgg
      901 caggtcggcc ccgctgccgg tcttgtagag ggaaggggtg gcgctgaccg ggtcggtcga
      961 gtcgcgcagc tccggggata cggatgaaac agcggaaaca gcggaaacag cggcgcgatc
     1021 gggggagccg tcgggggtgg gccccgagcc ggcgccctcc gcaggtgctc cgcccgtgtc
     1081 gcgctcgtcg ttcactcggc ggcgccttcc aacgcctccg cggccgaacc gtgcctgtcc
     1141 ttcgggacat acagctcgcc gcccgcggcg cggaactcgg cggccttggc ggccatgccc
     1201 tcctcggcgg ctgcggaaac ggcggccagt tcagccatgt cgcggacgtc ctgggtcagc
     1261 ttcatcgagc agaagtgggg gccgcacatc gagcagaagt gggcctcctt ggcgggctcg
     1321 gcgggcaagg tctcgtcatg aaaatccttg gcggtctcgg ggtcgagcga gaggttgaac
     1381 tggtcgtgcc agcggaactc gaagcgggcc ttcgagagcg cgtcgtcgcg gtcctgggcg
     1441 ccggggtggc ccttggcgag gtcggcggcg tgggcggcga tcttgtaggc gatcacgccg
     1501 tccttgacgt cctggcggtc ggggagcccg aggtgctcct tgggggtgac gtagcagagc
     1561 atcgcggtgc cgaagctgcc gatcattgcg gcgccgatcg ccgaggtgat gtggtcgtag
     1621 ccgggggcga tgtcggtggt caaaggcccg agcgtgtaaa acggcgcctc gtggcacagg
     1681 cgcagctgct cgtccatgtt ctccttgatc ttgtgcatcg gcacgtgacc ggggccctcg
     1741 atcatcacct ggacgtcgtg cttccaggcg acctgcgtca gctcgccgag cgtgcgcagc
     1801 tcgccgaact gggcctcgtc gttggcgtcg gcgatgcagc cggggcgcag gccgtcaccg
     1861 agcgagaagc tgatgtcgta cttctgcatc agggcgcaga tgcgctcgaa ctcggtgtag
     1921 aggaagttct ccttgtggtg gtgcaggcac cacttggcca tgatgctgcc gccgcgcgag
     1981 acgatcccgg tgacgcgctt cgcggtcaac gggatgtagc gcagaagtac tccagcgtgg
     2041 atcgtgaagt agtcgacgcc ctgctcggcc tgttcgtgca acgtctccat gaacacgtcg
     2101 atgttgaggt cctcggcctt gcccttgacc ttctcgagcg cctggtagat cgggacggtg
     2161 ccgatcggga ccggggcatt tcgcaggatc cactcgcggg tggtgtggat ctgcttgccg
     2221 gtggacaggt ccatcaccgt gtcggcgccc cagcgggtcg cccactgcag cttctcgacc
     2281 tcctcctcga tcgaggagcg cacggcggag ttgccgatgt tggcgttgat cttgaccagg
     2341 aagttgcggc cgatgatcat cggctcgagc tccgggtgct tgatgttggc cgggatgatc
     2401 gcgcggccga gcgcgacctc gctgcgcacg aactccgggt ccatgcgctc acggatcgcg
     2461 caaaagcgca tctcctcggt gatgacgccg cggcgggcgt agtgcatctg gctcaggttg
     2521 ccatctgcgg aactcacgcg cgcctcgatc cacgccttgc ggcgcggggg caagccctgg
     2581 cgggggtcga tgccctgcgg gccggtggtg tcgtagacga cgatgtccgg gttgccgccg
     2641 ctctgtgaga tgcgacgaaa cggcaccgcg agctcgccct cggtcacctt ggccgaattc
     2701 gggaagcttt ctaccaacgg cgcgatcgcg cggacgggcg tgacctccgg ggccgccagc
     2761 ggcaagctgc cgggacgggg cggggcgggg gtcagcgggg aatcgaccgc ctggggcggg
     2821 gtggcctcag cggccaggga cgggtgggtg tctgtgtttc gagggtccat ttgcgctttc
     2881 ctccgccggt acgacccgga tcaggttcaa ggggtatctc tcaggaccgc gcgcgcggac
     2941 cacccccagc gacgtgcgga cgatacggta aaaccgctgc ccgggccacg tgatccggcg
     3001 cggaacaggc gtcggcgcta cactcccgct ccatcgacga cacgctgtcg acggacgctg
     3061 tgggggcgac gagccccggg cgtccggccg gcccatgtga gggatcaaag gcaacgtttg
     3121 acgaggccca ggcctgcgtc gaggcgttgt cgaccccatg attttgcggg tttcattaca
     3181 ataatttcag tcggtgtaat cccactcccc gccatgtccc gatccccgtc ccgcgcccgg
     3241 tgcgcaggcc ggcccgggct ggcggtctca ccgaacgccg ccctgcggcc aagaaaaaag
     3301 gcgccaccat gcagatgcaa gcacaacttc ggaccctcgc tggcgcgctc gcgctggtgc
     3361 tcgcttgcgg cggtgaggat ggacccggca gcgcgaccga ggccggcagc agcagctcga
     3421 gcggcgaggt cgtgacgacg acgggtgaca tggtcacgac gaccggcgac atcccggcca
     3481 gctgcggcga cggcaaggtc gacccgggcg agggctgcga cgacggcgac gccaacggcg
     3541 aggcggcgga gtgcaccccc gagtgccagc tgcaggtgtg cggcgacggc cacgtcggcg
     3601 cgaaggaggg ctgcgacgac ggcaaccaga acgacgacga cgactgcacc aacgcgtgcg
     3661 cgctgtcgac gtgcggtgac ggcgtggtcc tgcagggcgc cgaggagtgc gacgacggcg
     3721 acgacgacaa cggcgacaac tgcctcaaga actgcaagct ggcggtctgc ggcgacggct
     3781 acgtgcgc
//
LOCUS       DASWNJ010001592         4470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_98978, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001592 DASWNJ010000000
VERSION     DASWNJ010001592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98978"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..645)
                     /locus_tag="VGB85_26420"
     CDS             complement(22..645)
                     /locus_tag="VGB85_26420"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEY0137659.1"
                     /translation="MITAKLLLLENKSWVQEKLALDPDYFTRFAASQQPDFLWIGCSD
                     SRIPTNEITVTTAGELFVHRNIANLIVENDINALSALQYAVDTLKVRHVIVCGHYGCG
                     GVRAAMGGEVPGPLGVWLRHIQAVYYTHRQELDALADETARWDRLVELNVTTQIEHLQ
                     RTEVVQGAWARGQALSLHGWVYRIASGVLQELVAVHGPGAAPGPAGG"
     gene            complement(642..2219)
                     /locus_tag="VGB85_26425"
     CDS             complement(642..2219)
                     /locus_tag="VGB85_26425"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEY0137660.1"
                     /translation="MPDISMTSGRRLWVSPRDDLPAAIVVFLVALPLCLGIALASGAT
                     PLAGITAGVIGGVVVGSLSGSSVSVSGPAAGLTAVVLAGITDLGGYPIFLTAVLLAGM
                     MQLGLGLARAGGVAHFVPVSVVRGMLAAIGLILMLKQIPHALGRDIDYEGDETFVEPG
                     GGNTFSDIAEAVMHVEPGALLVFLVSLGVLLGWSRIVPRIRNGNWFPASLLAVLAGTA
                     TNLALLVWWPEAALQASHRVAMPLLSSPGALAEAMQFPDFSHILDGAVWRMAVTLALI
                     ASIESLLSAEAADKLDPWRRITPMNRELRAQGVGNIVAALLGALPVTAVIVRSSANVA
                     AGGKTRLATIVHGILLLLSALFLAPWLNWIPLASLAAVLIVMGWRLARPRILLEQYRR
                     GVGQFVPYVVTIMAILLTDLLIGILIGIGVGVFFVVRSNFRSAVIVTRDGDTVLIRFA
                     KDVSFLNKPRLRGIFETITDGTSVIIDGTRAQFIDSDIVECIDDFVVSAPTRDITVEL
                     RRSPGSNNELFKTRPPV"
     gene            2503..3684
                     /locus_tag="VGB85_26430"
     CDS             2503..3684
                     /locus_tag="VGB85_26430"
                     /inference="COORDINATES: protein motif:HMM:NF020932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HEY0137661.1"
                     /translation="MLAPRPRFALLALLTAAACDSSGPASPWDDAEFTAQAADVRAQY
                     VAVVAATYADSLTSADAMQAEIEAFLAAPSAAGLDRARDAWLAAREVYGQTEVFRFYG
                     GPIDAEPDNLEGRINSWPLDEVYIDYVEGEPEGGLINDTATYPTIDRELLIARNAAGS
                     EDDISTGWHAIEFLLWGQDLRDDGPGERPYTDFVVGEDGTAANQDRRADYLAIATELL
                     VADLGTLSAAWQPGSANVDSFTAGDPKDALTKLLLGMGSLSGAELSGERMGVAYETRE
                     QEDEHSCFSDNTHRDLYTNALGIENVYLGQFGGVKGASLSQLVAGLDPELDRTLREQL
                     AASVAAMAAIPAPFDRAIQAPDGDPARQAVADGIAALKAQTATIAEVAARLEITLNLE
                     M"
     gene            3689..>4470
                     /locus_tag="VGB85_26435"
     CDS             3689..>4470
                     /locus_tag="VGB85_26435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEY0137662.1"
                     /translation="MSRRLAPLLSAIVLACEDAPALEPGEATPGGDTTVFDATGNAFS
                     LSARNMSFDRRQRFVVGNSFFNKNWVTAPSSTTSRDGLGPTFNARSCSTCHFKDGRGR
                     APGPGEDFSGLLLRLSIPGEEAHGGPLSAGPYGEQLQPQAVADVPAEGVPRVAYVERT
                     GSFADGAPYSLRAPTYTIDAPALGPLPADLMISPRVAPAMIGLGLLEAIPEADILARA
                     DPDDADGDGISGRANYVWDHAAGNKALGRFGWKANQPNVRQQT"
BASE COUNT          709 a         1699 c         1455 g          607 t
ORIGIN      
        1 cacgaagttc acgaggatcg atcagcctcc ggccggcccc ggcgcggcgc ccgggccgtg
       61 gaccgcgacc agctcctgca gcaccccgct ggcgatccgg tacacccagc cgtgcaggct
      121 cagcgcttgc ccccgcgccc acgccccctg gacgacctcg gtgcgctgca ggtgctcgat
      181 ctgcgtcgtc acgttgagct cgacgaggcg gtcccagcgg gcggtctcgt cggccagagc
      241 gtcgagctcc tggcgatgcg tgtaatagac cgcctgaatg tgccgcaacc acaccccgag
      301 cggacctggc acctcgccgc ccatcgccgc gcgtacgcct ccgcagccgt agtgcccgca
      361 gacgatcacg tgacggacct tgagggtgtc gaccgcgtac tgcaacgcgc tgagcgcgtt
      421 gatgtcgttc tcgacgatca ggttggcgat gttgcggtgc acgaacagct cgcccgccgt
      481 cgtcaccgtg atctcgttgg tcgggatccg gctgtccgag cagccgatcc acaggaagtc
      541 cggctgctgc gacgcggcga agcgggtgaa gtaatcgggg tcgagggcga gcttctcttg
      601 cacccaggac ttgttctcga gcagcagcag cttggcggtg atcatacggg gggtcgggtc
      661 ttgaacagct cgttgttgct accggggctg cggcgcagct ccacggtgat gtcgcgggtc
      721 ggggccgaca cgacgaagtc gtcgatgcac tcgacgatgt cgctgtcgat gaactgcgcg
      781 cgcgtgccgt cgatgatcac cgaggtgccg tcggtgatcg tctcgaagat cccgcgcagg
      841 cgcggcttgt tgaggaacga gacgtccttg gcgaagcgga tcagcacggt gtcgccgtcg
      901 cgcgtcacga tcaccgccga gcggaagttg ctgcgcacca cgaagaacac gccgacgccg
      961 atcccgatca ggatcccgat caggaggtcc gtcagcaaga tcgccatgat cgtcaccacg
     1021 tagggcacga attgcccgac gccgcggcgg tactgctcga gcaagatccg cggccgcgcc
     1081 agccgccagc ccatcacgat cagcaccgcc gccagcgacg ccagcgggat ccagttgagc
     1141 cagggcgcga ggaacagcgc gctgagcagc agcaagatcc cgtgcacgat cgtcgccagc
     1201 cgcgtcttgc cgccggccgc gacgttggcc gacgagcgca cgatcaccgc cgtcaccggc
     1261 agcgcgccga gcagcgccgc gacgatgttg ccgaccccct gggcccgcag ctcgcggttc
     1321 atcggcgtga tccggcgcca cgggtcgagc ttgtcggccg cctccgcgga cagcaggctc
     1381 tcgatgctgg cgatcagcgc cagggtcacg gccatgcgcc acaccgcgcc gtcgaggatg
     1441 tgcgagaagt ccgggaactg catcgcctcc gcgagcgcgc ccgggctgct cagcagcggc
     1501 atcgccaccc ggtggctcgc ctgcagggcc gcctccggcc accagacgag caacgcgagg
     1561 ttggtcgcgg tgccggcgag caccgcgagc agcgacgccg ggaaccagtt gccgttgcgg
     1621 atccgcggga cgatccgcga ccagccgagc agcacgccca gcgagaccag gaagaccagc
     1681 agcgcgcccg gctcgacgtg catcaccgcc tcggcgatgt ccgagaaggt gttgccgccg
     1741 cccggctcga cgaaggtctc gtcgccctcg tagtcgatgt cgcggccgag cgcgtgcggg
     1801 atctgcttga gcatcaggat caggccgatc gccgcgagca tcccgcggac cacgctgacc
     1861 ggcacgaagt gcgcgacgcc gccggcccgc gccaggccga gcccgagctg catcatgccc
     1921 gccagcagca ccgcggtgag gaagatcggg tagccgccga gatcggtgat ccccgcgagc
     1981 accaccgcgg tcagacctgc cgccggtccg ctgacggaca ccgacgagcc gctgagcgat
     2041 ccgacgacca cgccgccgat cacgcccgcg gtgatgccgg ccagcggcgt cgcgcccgac
     2101 gcgagcgcga tgccgaggca cagcggcagg gccaccagga acacgacgat cgccgcgggg
     2161 aggtcgtcac gcggcgacac ccagagccgc cgaccggagg tcatcgagat gtcaggcacg
     2221 acgagcaccc tatagcagcg ctttcgctat ccgacgccgc cagcttgaag gtcaatttac
     2281 tcctgacacg catcgggtga agcgactgtc gcgtcactca cttcctccca ggccgccggg
     2341 accggaacct gtctcttggg tccggtccgg acgacccgtc tgcagccccg tccccacggc
     2401 tcgcgccgtt tgcgcagact catgaggggc cccggatcgt ccacgcgagg tcgcgcacgt
     2461 tgacattgaa aacgaatttc aatatttctc tatagccccg tgatgctcgc tccacgccca
     2521 cgcttcgccc tcctcgccct cttgaccgcc gccgcatgtg actcgtcggg gcccgcctcg
     2581 ccgtgggacg acgccgagtt caccgcgcag gccgccgacg tacgcgcgca gtatgtggcc
     2641 gtcgtggccg ccacctacgc cgactcgctg accagcgccg acgcgatgca ggccgagatc
     2701 gaggcctttt tggccgcgcc ctcggccgcc ggcctcgatc gggcgcgcga cgcctggctc
     2761 gccgcgcgcg aggtctacgg ccagacggag gtgttccgct tctacggcgg cccgatcgac
     2821 gccgagcccg acaacctcga gggtcgcatc aacagctggc cgctcgacga ggtctacatc
     2881 gactacgtcg agggcgagcc cgagggcggc ctcatcaacg acaccgcgac ctacccgacg
     2941 atcgaccgcg agctcctgat cgcccgtaac gccgccggca gcgaggacga catctcgacc
     3001 ggctggcacg cgatcgagtt cctgctgtgg ggtcaggacc tgcgcgacga cggccccggc
     3061 gagcgcccat acaccgactt cgtcgtcggc gaggacggca ccgcggccaa ccaggaccgc
     3121 cgcgccgact acctcgcgat cgccaccgag ctgctggtcg ccgacctcgg caccctctcc
     3181 gccgcgtggc agccgggctc ggccaacgtc gacagcttca ccgccggcga ccccaaggac
     3241 gcgctgacca agctgctgct cggcatgggc agcctcagcg gcgccgagct ctcgggcgag
     3301 cgcatgggcg tcgcctacga gacccgcgag caggaggacg agcactcctg cttcagcgac
     3361 aacacccacc gcgacctcta caccaacgcg ctcgggatcg agaacgtcta cctcggccag
     3421 ttcggcggcg tgaagggtgc cagcctctcg cagctggtcg ccggcctcga ccccgagctc
     3481 gaccgcacgc tgcgcgagca gctcgccgcc agcgtcgcgg cgatggccgc gatccctgcc
     3541 cccttcgacc gcgcgatcca ggcccccgac ggtgaccccg cgcgccaggc cgtcgccgac
     3601 ggcatcgccg ccctcaaggc ccagaccgcg acgatcgccg aggtcgccgc gcgcctcgag
     3661 atcaccctca acctcgagat gtgaaccgat gtcccgtcgc ctcgccccgc tgctgtccgc
     3721 gatcgtgctc gcctgcgagg acgcgcccgc gctcgagccc ggcgaggcga cgcccggcgg
     3781 cgacacgacc gtgttcgacg ccaccggcaa cgccttctcg ctgtcggccc gcaacatgag
     3841 cttcgatcga cgccagcgct tcgtcgtcgg caactcgttc ttcaataaaa actgggtgac
     3901 cgcgccgagc tcgaccacct cgcgcgacgg cctcggcccg accttcaacg cccgctcgtg
     3961 ctcgacctgt cacttcaaag acggccgcgg ccgcgccccc gggcccggcg aggacttctc
     4021 cggcctgctg ctgcgcctca gtattcccgg cgaggaggcc cacggcggcc cgctctccgc
     4081 cggcccctac ggcgagcagc tccagcccca ggcggtcgcc gacgtgcccg ccgaaggcgt
     4141 cccgcgcgtc gcctacgtcg agcggaccgg cagcttcgcc gacggtgcgc cctactcgct
     4201 tcgcgccccg acctacacga tcgacgcgcc cgcgctcggt cccctgcccg ccgacctcat
     4261 gatctcgccg cgcgtcgccc cggcgatgat cggcctcggt ctgctcgagg cgatcccgga
     4321 ggccgacatc ctcgcgcgcg ccgaccccga cgacgcggac ggcgacggaa tctcgggccg
     4381 cgcgaactac gtgtgggacc acgccgccgg caacaaggcc ctcggccgct tcggctggaa
     4441 ggccaaccag ccgaacgtgc gccagcagac
//
LOCUS       DASWNJ010001593         3283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_560094, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001593 DASWNJ010000000
VERSION     DASWNJ010001593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560094"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(635..937)
                     /locus_tag="VGB85_26440"
     CDS             complement(635..937)
                     /locus_tag="VGB85_26440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137663.1"
                     /translation="MALTIAFVAWLAAAGVVIGFCLGGLLGPRIFGSLGGHGLGDLGY
                     LFGGAAVGAAVGVLVGIYGSIAWTPQQHARVATWAGLAGLIVAGVTMLLVNSFGAW"
     gene            complement(1032..1298)
                     /locus_tag="VGB85_26445"
     CDS             complement(1032..1298)
                     /locus_tag="VGB85_26445"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY0137664.1"
                     /translation="MSDGAELDSLRTAQVCEAAGVTDATVMRWAKQGVMPPYERVFRG
                     RRGSFARWPKHAAEQAARVKAQLDAGRSFEEIRQALGRGEFKTG"
     gene            1637..2308
                     /locus_tag="VGB85_26450"
     CDS             1637..2308
                     /locus_tag="VGB85_26450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137665.1"
                     /translation="MPTTSTWLASDRRGDLGLFSLERWGMVPEDAALLWEGARGDEVW
                     RALHVAWIADMAPRSVLTMHTLVTIPEAEGPHLVAFRERREDLPGAHFITDTVARFEQ
                     IGAELGAQLAEDEGVLGSISLDLASFGDFVGAAVYRYSRWGDDAVYRRAGAPQAAMKR
                     SSLPEDLEGVAHLDTEFAVQERLDLVTILEPGRSYEAGTRPSPVAPGPAAAASRPWWR
                     RLRGG"
     gene            2477..>3283
                     /locus_tag="VGB85_26455"
     CDS             2477..>3283
                     /locus_tag="VGB85_26455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEY0137666.1"
                     /translation="MRWAQPFLICVALSLAATEATAASRGALIWDGEYWTFGYGALEQ
                     GLAAGGANPVERASVLPADLSAYNLVIVVLPSSTPDAAATDALVDFVAGGGALVLVSD
                     FEGYAPEPSGIFNAMLTDYDVDARFGPGTIYNCSSIVTTSPHPLMAGVVELHGAASDF
                     IVPGPATEIIVQALAQEPILAVERNIVLLGDSNAINDYVCAPAGPNGPFFANLPAFAC
                     DIDGDGVSNGSCDGDDPNDLDPAVSGDDGGSGGEESGGDTTTTTDPTGEPG"
BASE COUNT          484 a         1092 c         1184 g          523 t
ORIGIN      
        1 gcccgctctg ccggcgtcgt gttacacccc gctcgccgcc gagcgggccc tcgaccatct
       61 gatcaagtag tactagggcg cgtaaggcag cctggatcgg tcgtgcggga cctttgccgg
      121 gtacggctct ctggcggcag aggcagcgcc cgcggtgtcg ggcgacgcgc gaacggggcc
      181 accggcgccg ccagcagggc ctcgaccttg ggccaggtcc cgtccgcgca gcaacgcttg
      241 taccggcgcc agcacgtcgt agagctgcgt aggtcgccgg gatgtccgac cagtgcgcct
      301 gcgtgtggag cagccagcgg atgccgtcca cgacatgccg caactccacc gcttgccgcc
      361 gcgcggtcgg tcggcgtcca cgagggtcac gatcgccgcc cactggacgt cgcgtagcgg
      421 ctcgaacggc gccacgggca gcgcggaggg catgttggtc gccgcggcga tgatgcccgc
      481 ggtgtgctcg agcgcctcgc ggacgacgcg gttggccaag tgggcgtagc gcttggtcgt
      541 gctgggctgg gggtgcccca gtatctcgcc gacgatcgct agggagacgc ggagatccgc
      601 gcccatatgc gggcccggat acacaggccc ggtctcacca ggccccgaag ctgttgacga
      661 gcagcatcgt gacaccggcg acgatgagtc cggcgagccc ggcccaagtg gccactcgtg
      721 cgtgctgttg tggggtccag gcgatgctgc cgtagatgcc gacgagcacg cccaccgcgg
      781 cgccgacggc tgcgcccccg aacaggtagc cgaggtcgcc cagcccgtgt ccgccgaggc
      841 tgccgaagat ccgcggtccg agcaaaccac cgaggcaaaa accgatgacc acaccggctg
      901 cggcgagcca cgccacgaag gcgatggtga gggccatgga cttcagccct ggcatcggag
      961 cgctcccgcg ggaaacattc ggacgaacat cctgcatcct accgccgatc agagctctcg
     1021 ccacgtgggg cctaccccgt cttgaactcg ccgcggccga gggcttgccg gatctcctcg
     1081 aatgaccgcc cggcgtccag ttgcgccttg acccgcgcag cctgttcggc tgcgtgtttc
     1141 ggccagcggg cgaaggagcc gcggcggccg cggaagacgc gttcgtaggg cggcatgacg
     1201 ccctgcttgg cccagcgcat gacggtggcg tcggtgacac cggcggcctc gcagacctgg
     1261 gccgtgcgca gggagtccag ttccgcgccg tcgctcacgg gcgcgagtct atgggccgac
     1321 catacgtgct ccaagcttga agtttcatgg gttcgacgtg tcctctacga ggagtccagg
     1381 aggatacgag accatggaag atcatgacga cgattcatgg gcgcgaggcg gcgttccagc
     1441 gagcccgaga gggcctcagg gtgccgatac aggcgatttt ggcggggagg gccctggcgc
     1501 gcggatggcg ttgttggtgc tgccccgcgg ggtgacgctc gaggtgcacg tccccgggac
     1561 acgcgagttg cttctccgct cggcgtagga ctcgatagca gccgtctgac cccgggacat
     1621 ggtagggacg atctgcatgc cgacgacgtc gacgtggctc gcgagcgacc gtaggggcga
     1681 cctgggcctc ttctcgctgg agcgctgggg catggttccc gaagatgcgg cgctgttgtg
     1741 ggagggagcg cgtggcgacg aggtctggcg cgcgctccac gtcgcctgga tcgccgacat
     1801 ggcgccgcgg tcggtgctga cgatgcacac attggtgacg atcccggagg cggagggccc
     1861 tcatctcgtc gcattcaggg agcgacgtga agatctgccg ggcgcgcatt tcatcaccga
     1921 cacggtggct cgcttcgagc agatcggggc cgagctcggg gcgcagctgg ccgaggacga
     1981 gggggtgctc ggctcgatca gcctcgatct ggcgtcgttc ggagatttcg tgggcgcggc
     2041 ggtgtatcgc tattctcggt ggggggacga cgcggtctat cgccgcgccg gagccccgca
     2101 ggcagcgatg aagcgctcgt ccctgccgga ggatctcgag ggtgtcgcgc atctcgacac
     2161 ggagtttgca gtgcaggagc gtctcgatct cgtcaccatc ctcgaacccg ggcgctcgta
     2221 cgaggcgggc actcggccat caccggtcgc gccgggtccg gcagccgcgg cgtcgcgacc
     2281 gtggtggcgt cgccttcgtg gcggttgacg ccgggcacgt attgtgcggc atcgggatcg
     2341 acaccacgca tggtggacca tcgaggcgtg acggaggcgg actccaagtg gtccggcacg
     2401 cgtacggatc tggccgtcgt tgttcgcggc tccgcgcggg gcttgaggtt cacgcacatc
     2461 cgggctagtc acgcacatgc gctgggccca gccgttcctc atatgcgtcg cgctgtctct
     2521 ggccgccacg gaggcgaccg ccgcgagtcg cggcgcgttg atctgggacg gtgagtactg
     2581 gaccttcggg tacggcgcgc tcgagcaggg ccttgcggct ggcggcgcga acccggtcga
     2641 gcgggcgtcc gtgctgccgg ccgacctctc cgcctacaac ctcgtgatcg tcgtgttgcc
     2701 ctcgtccacg ccggacgcgg cggccaccga cgcgctcgtc gattttgtcg ccggcggcgg
     2761 cgcgctcgta ctcgtcagcg acttcgaggg gtacgcgccc gagccgtccg gcatctttaa
     2821 cgccatgctc accgattacg acgtcgacgc gcgcttcggc ccgggcacga tctacaattg
     2881 cagctcgatc gtgacgacga gcccgcaccc gctgatggcc ggggtcgtcg agctccatgg
     2941 cgccgcgtcg gacttcatcg tccctggacc ggccacggag atcatcgtgc aggcgctggc
     3001 gcaggagccg atcctggcgg tcgagcggaa catcgtcctg ctcggcgatt cgaacgcgat
     3061 caacgattac gtctgcgcgc ccgcggggcc gaatggcccg ttcttcgcca acttgccggc
     3121 gttcgcctgc gacatcgacg gtgacggggt cagcaacggc tcctgcgacg gcgatgaccc
     3181 aaacgacctc gatccggcgg tctccggcga cgacggcggc tcgggtggtg aggagagcgg
     3241 cggtgacacg accacgacga ccgacccgac cggcgaaccc ggc
//
LOCUS       DASWNJ010001594         1553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_28518, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001594 DASWNJ010000000
VERSION     DASWNJ010001594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28518"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VGB85_26460"
     CDS             <1..625
                     /locus_tag="VGB85_26460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137667.1"
                     /translation="QEQRVVDELVLAWGSRPGEPVRLHLSQQTDKGEGREVVILDEQV
                     YTRLARRGWYARALDSELHWTWLDEAQHCVHDVVELAAPALAVTAEESGDTVEVTLAR
                     ADTVDPTRIAAGHGREWRQRTEIGEVTGSITLDRTTGLWRSAEVTVRYSLRDALERPQ
                     RGETQLTATAAPAESVAIAAPDTASPVPERIRPELERQRLLGGLAGS"
     gene            684..1403
                     /gene="rsfS"
                     /locus_tag="VGB85_26465"
     CDS             684..1403
                     /gene="rsfS"
                     /locus_tag="VGB85_26465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEY0137668.1"
                     /translation="MNKAASSRAELPANLQRALDLAIAAKGVGPVILHLTEVAGYTDH
                     ALIISGRSDRHVGGITDAIVSGLKAQGVAPIGSDGLDEHLWDLIDYDDFLVHVFYHPV
                     RKHYDLESMWIDAPRIELDLPSDVMDTSGLEELAPPDPMPAFRGGSFGAFEGELDNEE
                     DPDLVGITGPGELSADAEVIPDEEDYDDDDADDLDEFEDDGDEFDDDDALDGGDELDP
                     AADAGDAASPAAPAPGPARRK"
BASE COUNT          235 a          614 c          514 g          190 t
ORIGIN      
        1 gcaagagcag cgcgtcgtcg acgagctcgt gctcgcctgg ggctcgcgcc ccggtgagcc
       61 cgtgcgcctg cacctgtccc aacagacaga caagggcgag ggccgcgagg tcgtcatcct
      121 cgacgagcag gtctacaccc gcctcgcgcg ccgcggctgg tacgcgcgcg cgctcgactc
      181 ggagctgcac tggacctggc tcgacgaggc ccagcactgc gtgcacgacg tcgtcgagct
      241 cgccgccccg gccctcgcgg tgacggccga ggagtcgggc gacaccgtcg aggtcacgct
      301 cgcccgcgcc gacacggtcg acccgacccg catcgccgcc ggccacggcc gcgagtggcg
      361 ccaacgcacg gagatcggcg aggtcaccgg cagcatcacg ctcgaccgca ccacgggcct
      421 gtggcgcagc gccgaggtca ccgtgcgtta cagcttgcgt gacgcgctcg aacgccccca
      481 gcgcggcgag acgcagctca ccgcgaccgc ggcccccgcc gaatccgttg cgatcgccgc
      541 accggacacc gccagcccgg tcccggagcg catccgcccg gagctcgagc gccagcgcct
      601 gctcggtgga ctcgccggct cgtagcactc acttcggagg ggcgccggtt gtcgccggcg
      661 caaagccgcg ctacaaggcg tgcgtgaaca aggccgcctc ctctcgcgcc gaactgcccg
      721 cgaacctgca acgcgctctc gacctcgcga tcgccgccaa gggggtcggc ccggtcatcc
      781 tgcatctcac cgaggtcgcc ggttacaccg accacgcgct gatcatctcg ggccgctcgg
      841 accgccacgt cggcggtatc accgacgcga tcgtcagcgg actcaaggcg cagggcgtcg
      901 ccccgatcgg cagcgacggc ctcgacgagc atctgtggga cctgatcgac tacgacgact
      961 tcctcgtcca cgtcttctat cacccggtcc gcaagcacta cgacctcgag agcatgtgga
     1021 tcgacgcccc gcgcatcgag ctcgacctgc ccagcgacgt catggacacc tccggcctcg
     1081 aggagctcgc gccgcccgac ccgatgcccg ccttccgcgg cggcagcttc ggggccttcg
     1141 agggcgagct cgacaacgag gaggaccccg acctcgtcgg catcaccggc cccggtgagc
     1201 tctccgccga cgccgaggtc atccccgacg aagaggatta cgacgacgac gacgccgacg
     1261 acctcgacga gttcgaggac gacggcgacg agttcgacga cgacgacgcc ctcgacggcg
     1321 gcgatgagct cgacccggcc gccgacgccg gcgacgcggc gtcccccgct gccccggccc
     1381 ccggccccgc gcgtcgcaag tgatgcgcct cgccgtcgtc gctgccggca agctgcgcga
     1441 cgccggcctg cagggcctgt gtgacgacta cggcaagcgg atccagccgt tcatgccgct
     1501 cgaggtcgtc gaggtccgcg acgccgcggg cctgcgcgcc cgctgggccc gcg
//
LOCUS       DASWNJ010001595         3464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_664791, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001595 DASWNJ010000000
VERSION     DASWNJ010001595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664791"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VGB85_26470"
     CDS             complement(<1..708)
                     /locus_tag="VGB85_26470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137669.1"
                     /translation="MPTKPGEERESEAAAAVVPAADGPEKGAGPQVRVGEAGEGSAAR
                     GSADTGPAPDSRLTRAAMLLPVIGAPAGLMFAIFASLMISGWHVEHSAIVQVDTLWRP
                     GERVALRVHVQDGDGVGIPDASVQASLVRGEQTLALAQLADATGGGATQGTFEVPGWA
                     PGPAELVLAIEGGRQRFRETVPVRLDPAPEARRGTVMVSSSIKNWADDTEPQPEKLRI
                     ALRPIGRLAAGFENTLLV"
     gene            805..2118
                     /locus_tag="VGB85_26475"
     CDS             805..2118
                     /locus_tag="VGB85_26475"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEY0137670.1"
                     /translation="MKISVIGTGYVGLVAAAVFADFGNDVLCADIDAAKIESIRQGKL
                     PIFEPGLQPLVEHNIAAGRLRFTTSNAEAAVHGDIIFMAVGTPTLGSDGAADLSFLYA
                     AAAEIGRHIDRHTIIVDKSTVPVGTAERITRIVGEQTKVPFVVVSNPEFLKEGAAVDD
                     FMHPDRIVIGTTDEHARTVLHRLYQPFCRTNDRMIFMDARSAELTKYACNAYLAARIS
                     FMNDMANLADSVGADIELVRRGMGSDPRIGPKFLFPGVGYGGSCFPKDIRAVLTTARE
                     YGHNLEIVASAERINEAQKLVLVRKLRKHFDNDIAGKTFGVWGLAFKPNTDDIREAPA
                     LKIVRTLAGDGARLKLHDPEGGPNFMAALGESVARSCTLVDDPYEAAEGSHGLLLVTE
                     WREYRSPDFERLKTIMARPSLFDGRNQWDRELLEQLGFFYTGIGR"
     gene            2182..3042
                     /locus_tag="VGB85_26480"
     CDS             2182..3042
                     /locus_tag="VGB85_26480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137671.1"
                     /translation="MARKILRRLVADGQLYLYTTGWTNDLDGERVVSVALFHSEVGRE
                     QHPRGKPMRAHFLSREAEYPAPGAVAKPADVRAVLDQALALGWDGRREGWLLPACGLE
                     RPDLVLSTKTRLKAWAGEAPIFVAHIEEADIAGRLATALAVPPVPGARGAAEAQWQDD
                     RRYLLRSRWGTAVHVYTRSVAELVHVLTTLHRRAPQLGASLQAIPTRDVHVDFPLDQV
                     VPPEHWATLQDATLHHGARPTDQLWSFTAPEGPAIESYEFDPDAPDRLRRWASLRDAG
                     SVERRTPAPA"
     gene            3084..>3464
                     /locus_tag="VGB85_26485"
     CDS             3084..>3464
                     /locus_tag="VGB85_26485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137672.1"
                     /translation="MAKAHDTWKVLPHRPIEVVAENLWRVEGKLEGMPLKRVMTIARR
                     SDGDLVIHNAMALDDASMARIDEWGRVAWIIVPNGYHRLDARVFKDRYQAARVLCPRG
                     GRAKVEEVVPVEGAYEDFTVDPDVA"
BASE COUNT          503 a         1402 c         1086 g          473 t
ORIGIN      
        1 gaccagcagc gtattttcaa aacccgcggc gaggcgcccg atcggccgca gcgcgatccg
       61 caatttctcc ggctgtggct cggtgtcgtc ggcccagttc ttgatcgacg acgacaccat
      121 caccgtgccg cgccgggcct cgggcgcggg gtcgaggcgc accggcaccg tctcgcggaa
      181 gcgttgccgg ccgccctcga tcgccagcac cagctcggcg ggcccgggcg cccatccggg
      241 cacctcgaag gtcccctgcg tcgcgccgcc gccggtcgcg tcggccagct gcgcgagcgc
      301 cagggtctgc tcgccgcgca ccaggctcgc ctgcacgctc gcgtccggga tcccgacgcc
      361 gtcgccgtct tgcacatgca cgcgcagcgc gacgcgctcg ccgggccgcc acagcgtgtc
      421 gacctgcacg atcgccgagt gctcgacgtg ccagccggag atcatcaggc tggcgaagat
      481 cgcgaacatc agccccgcgg gcgcgccgat gaccggcagc agcatcgccg cgcgcgtcag
      541 cctcgagtcc ggcgccggtc cggtgtccgc cgacccgcgc gcggccgatc cctccccggc
      601 ctcgccgacg cggacctgcg gtcctgcccc cttctccggc ccatctgccg caggaacgac
      661 cgccgcagcg gcctcggact cgcgctcttc cccgggcttg gtcggcatca cgcacctcgg
      721 cgggcgcggg cgagccttca cggcgccgcg acccagcttc gcagcgtagc agaccccgcg
      781 gcgttgcagt atcctcgcgg ccccatgaag attagcgtca tcggtactgg atatgtcggc
      841 ctcgtcgcgg cggccgtgtt cgcggacttt ggtaacgacg tgctctgcgc ggacatcgac
      901 gccgccaaga tcgagtcgat tcggcaaggc aagctgccca tttttgagcc cggcctccag
      961 ccgctggtcg agcacaacat cgccgccggc cgcctgcgct tcaccaccag caacgccgag
     1021 gccgcggtcc acggcgacat catcttcatg gcggtcggca cgccgacgct cggatccgac
     1081 ggcgccgccg acctctcctt cctgtacgcg gccgccgcgg agatcggccg gcacatcgac
     1141 cgccacacga tcatcgtcga caagagcacc gtccccgtcg gcaccgccga gcgcatcacc
     1201 cggatcgtcg gcgagcagac caaggtcccc ttcgtcgtcg tcagcaaccc cgagttcctc
     1261 aaggagggcg ccgccgtcga cgacttcatg caccccgacc gcatcgtcat cggcaccacc
     1321 gatgagcacg cgcgcacggt cctgcaccgc ctctaccagc cgttttgccg caccaacgat
     1381 cgcatgatct tcatggacgc gcgatcggcc gagctgacca agtacgcctg caacgcctac
     1441 ctcgcggcgc gcatctcgtt catgaacgac atggccaacc tcgccgacag cgtcggcgcc
     1501 gacatcgagc tcgtgcgccg cggcatgggc tccgacccgc gcatcggccc caagttcctg
     1561 ttccccggcg tcggctacgg cggctcgtgc ttccccaagg acatccgcgc cgtgctgacc
     1621 accgcccgcg agtacggcca caacctcgag atcgtcgcct cggccgagcg catcaacgag
     1681 gcccagaagc tcgtcctcgt caggaagctg cgcaagcact tcgacaacga catcgccggc
     1741 aagacctttg gcgtgtgggg cctggccttc aagcccaaca ccgacgacat ccgcgaggcc
     1801 cccgccctca agatcgtgcg caccctcgcc ggcgacggcg cccgcctcaa gctccacgac
     1861 cccgagggcg gccccaactt catggccgcg ctcggcgagt ccgtcgcccg ctcgtgcacg
     1921 ctggtcgacg acccctacga ggccgccgag gggtctcacg gcctcctgct cgtcaccgag
     1981 tggcgcgagt accggagccc cgacttcgag cgcctcaaga cgatcatggc ccgcccctcg
     2041 ctgttcgacg gcaggaacca gtgggaccgc gagctgctcg agcagctcgg cttcttctac
     2101 accggcatcg gccgctgacc ggcgccgcac cgtcctcgcc ggcttgccat cgggccggct
     2161 cccggtccat ggttcgcgcc cgtggcccgc aagatcctcc gccgcctggt cgccgacggc
     2221 cagctctatc tctacacgac cggctggacg aacgacctcg acggcgagcg cgtcgtctcc
     2281 gtcgccctgt tccacagcga ggtcggccgc gaacagcacc cgcgcgggaa accaatgcgc
     2341 gcccacttcc tgtcccgcga ggccgagtac ccggcgcccg gcgccgtcgc caagccggcc
     2401 gacgtccgcg ccgtgctcga ccaggccctc gccctcggct gggacggccg ccgcgagggc
     2461 tggctgctgc ccgcctgcgg cctcgagcgc cccgacctcg tgctgagcac gaagacccgc
     2521 ctcaaggcct gggccggtga ggccccgatc ttcgtcgccc acatcgagga ggccgacatc
     2581 gccgggcggc tcgccacggc gctcgccgtc ccgcccgtgc ctggggcccg cggcgccgcc
     2641 gaggcgcagt ggcaggatga ccgccgctac cttctgcgct cgcgctgggg caccgccgtg
     2701 cacgtgtaca cgcgcagcgt cgccgagctc gtgcacgtgc tgacgaccct gcaccgccgc
     2761 gccccgcagc tcggcgcgtc gttgcaggcg atcccgaccc gggacgtcca cgtcgacttc
     2821 ccgctcgacc aggtcgtccc gcccgaacac tgggcgacgc tgcaggacgc gaccctgcac
     2881 cacggcgcgc ggccgaccga ccagctgtgg agcttcaccg cccccgaggg ccccgcgatc
     2941 gagtcctacg agttcgaccc cgacgccccc gaccgcctgc gccgctgggc cagcctgcgc
     3001 gacgcgggat cggtcgaacg caggaccccc gcgcccgcct gaccgcttgt cgcacccccg
     3061 gcagtgcggc atatcatgcc ggcatggcca aggcgcacga cacctggaag gtcctccccc
     3121 accgcccgat cgaggtcgtc gccgagaacc tctggcgcgt cgagggcaag ctcgagggca
     3181 tgccgctcaa gcgcgtcatg acgatcgccc gccgcagcga cggcgacctc gtgatccaca
     3241 acgcgatggc cctcgacgac gcctcgatgg cccgcatcga cgagtggggc cgcgtcgcct
     3301 ggatcatcgt ccccaacggc taccaccgcc tcgacgcgcg cgtttttaaa gaccgctacc
     3361 aggcagcccg cgtgctgtgc ccgcggggcg gccgcgccaa ggtcgaggag gtcgtccccg
     3421 tcgagggcgc ctacgaggac ttcaccgtcg accccgacgt cgcc
//
LOCUS       DASWNJ010001596         2408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_594007, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001596 DASWNJ010000000
VERSION     DASWNJ010001596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594007"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VGB85_26490"
     CDS             <1..555
                     /locus_tag="VGB85_26490"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEY0137673.1"
                     /translation="ERGNLPIVTGVEREKLVAGDPAAAQVIQRALSVLATYNDKARPR
                     LGEIHIGEGGEALLYTEKTGTLLRLGRGPVEDRLARFDALRAALGDRADKLSVVHLDT
                     APGSDRPARVIASFVDQAEAATLLAQASKVAVPTVAQDLEDMAQKPAQNITPAPGKPA
                     KKQGKPVKGKQGAHKFASGIPKYD"
     gene            568..1848
                     /gene="ftsA"
                     /locus_tag="VGB85_26495"
     CDS             568..1848
                     /gene="ftsA"
                     /locus_tag="VGB85_26495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY0137674.1"
                     /translation="MAEVGEVIVGLDIGTTKVAAIIGEVTENGVDIIGVGTCPSEGLR
                     QGVVVNIEATTNSIAQAIQEAEQMAAVEVQSVFCGIAGGHIRGFSSLGQVSMRNREVT
                     DLDVARVIEQAKAVNIPLDRQIIHTLPLEYMIDGQGQIKDPIGMSGVRMEVRAHVITA
                     ATTSVQNIIKCCNRAGLDVIDVVLEPLASATSVLHDDERELGVILIDIGGGTTDLAIY
                     AEGQNVFTSVIVMGGHRITQDVAHGLHTSVAEAEAIKRKHGCALVSMVDGDAQIEVAG
                     VGPRPPRSFPRRFLSEVIEPRVEEILLLAQEELQQSGYMELAGSGIVLTGGQSQMEGM
                     AEIVEDIFGLPVRRGLPVHGASFGRHAPALTEGGFAGVIHDPKFATGVGLVLYGAAHE
                     PMAVTHEPTHHRGQHGEGRPGIGKRFVGWMRDMF"
     gene            1959..>2408
                     /locus_tag="VGB85_26500"
     CDS             1959..>2408
                     /locus_tag="VGB85_26500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137675.1"
                     /translation="MLLAALLLLQTPPSPAPPATEPPPTAVEAAPPAEAPAAQAPPGE
                     TQPATTTPDDPATDGDAEEPPSATNPAAVGPKEAPAPAHHAPAVGGGATVFDAKEPET
                     DLARSRYTLGKGYTLSSKDERYSLQIRARLQVRYDLEHYNVADKAMAQ"
BASE COUNT          406 a          904 c          807 g          291 t
ORIGIN      
        1 gagcgcggca acctcccgat cgtcaccggc gtcgagcgcg agaagctcgt cgccggcgac
       61 ccggcggccg cgcaggtgat ccagcgcgcg ctgtccgtgc tcgcgaccta caacgacaag
      121 gcgcgcccgc gcctcggcga gatccacatc ggcgagggcg gcgaggcgct gctctacacc
      181 gagaagaccg gcacgctgct gcgcctcggt cgcggcccgg tcgaggatcg cctggcccgc
      241 ttcgacgcgc tgcgggccgc gctgggcgac cgcgccgaca agctctcggt cgtgcacctc
      301 gacaccgccc ccggctccga ccggccggcg cgcgtgatcg ccagcttcgt cgatcaggcg
      361 gaggccgcga cgctgctggc ccaggcgagc aaggtcgccg tgcccacggt cgcgcaggac
      421 ctcgaggaca tggcccaaaa gccagctcag aacatcacac ccgcacccgg caagcccgcc
      481 aagaagcagg gcaagccggt gaagggcaag cagggggcgc acaagttcgc gtccgggatc
      541 cccaagtacg actagacgga gctcgtcatg gcagaagtcg gagaagtcat cgtcggcctg
      601 gacatcggca ccaccaaggt ggccgcgatc atcggtgagg tgacggagaa cggcgtcgac
      661 atcatcggcg tcgggacctg tccgtccgag ggcctgcgcc agggggtcgt cgtcaacatc
      721 gaggcgacca ccaacagcat cgcgcaggcg atccaggagg ccgagcagat ggccgccgtc
      781 gaggtgcagt cggtgttctg cgggatcgcg ggcgggcaca tccgcggctt tagcagcctc
      841 ggccaggtca gcatgcgcaa ccgcgaggtc accgacctcg acgtcgcccg cgtgatcgag
      901 caggccaagg cggtcaacat cccgctcgac cgccagatca tccacacgct gccgctcgag
      961 tacatgatcg acggccaggg ccagatcaag gacccgatcg gcatgagcgg cgtgcgcatg
     1021 gaggtccgcg cgcacgtgat cacggccgcg accaccagcg tgcagaacat catcaagtgc
     1081 tgcaaccgcg ccggcctcga cgtcatcgac gtcgtgctcg agccgctcgc cagcgcgacc
     1141 tcggtgctgc acgacgacga gcgcgagctc ggcgtgatcc tgatcgacat cggcggcggc
     1201 accaccgacc tggcgatcta cgccgagggc cagaacgtgt tcacctcggt gatcgtcatg
     1261 ggcggccacc gcatcaccca ggacgtcgcc cacggcctgc acaccagcgt cgccgaggcc
     1321 gaggcgatca agcgcaagca cgggtgtgcc ctggtctcga tggtcgacgg cgacgcccag
     1381 atcgaggtcg cgggcgtcgg ccctcgcccg ccgcgcagct tcccgcgccg cttcctctcc
     1441 gaggtcatcg agccgcgcgt cgaggagatc ctgctgctcg cccaggagga gctgcagcag
     1501 agtggttaca tggagctcgc cggcagcggc atcgtgctga ccggcggcca gagccagatg
     1561 gaaggcatgg cggagatcgt cgaggacatc ttcggcctgc cggtccgccg cggcctgccc
     1621 gtgcacggcg ccagcttcgg ccgccacgcc cctgctctca ccgagggcgg cttcgccggc
     1681 gtgatccacg acccgaagtt cgccaccggc gtcggcctcg tgctctacgg cgccgcccac
     1741 gagccgatgg ccgtcaccca cgagcccacc caccaccgtg gtcagcacgg cgagggccgc
     1801 cccggcatcg gcaagcgctt cgtcggctgg atgcgcgaca tgttctgagc tgtcgcaggc
     1861 ccgtcgtgtg acgggactgc aacaaccgcg gcgatctgtc tcgaaatcgt cacatgacgg
     1921 ctccgcgcgc gtgacgccga ggctacaggc tcgccgccat gctcctcgcc gcgctcctgt
     1981 tgctgcagac gccgccctcg cccgcgccac ccgccaccga gcccccgccg accgccgtcg
     2041 aggcggcccc tccggccgaa gcaccggccg cgcaggcccc tcccggcgag acgcagcccg
     2101 ccaccacgac accggacgat cccgcgaccg acggcgacgc agaggaacca ccgtccgcca
     2161 cgaaccccgc ggcggtggga cccaaggagg cgccggcgcc ggcccatcac gcgccggcgg
     2221 tcggcggcgg cgcgaccgtg ttcgacgcca aggagcccga gaccgacctc gcccgctcgc
     2281 gctacacgct cggcaagggc tacacgctgt cgagcaagga cgagcgctac agcctgcaga
     2341 tccgcgcgcg cttgcaggtc cgctacgacc tcgagcacta caacgtcgcc gacaaggcga
     2401 tggcgcag
//
LOCUS       DASWNJ010001597         1666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_594044, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001597 DASWNJ010000000
VERSION     DASWNJ010001597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594044"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(249..914)
                     /locus_tag="VGB85_26505"
     CDS             complement(249..914)
                     /locus_tag="VGB85_26505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137676.1"
                     /translation="MPRTLAALRAAPVAWALGAGVCVACTTEVRLPCDGVCGPGTECV
                     ADRCEAVAAPPPAEEPTGKRKRRGKRGRKGTPAVAGDPGAAEAAGEVEETAAAPPPFV
                     PVDDSRVPQFSNAEPQALDMKAGSERLSESELDRHFAQVTPQIQRCVTTASAYGDIGA
                     GSLKFKLRVLPSGKVESVSLTAPASLRVWGIPSCARRAVYDHRFPSFDGAAMAVTFGV
                     DID"
     gene            1048..1410
                     /gene="queF"
                     /locus_tag="VGB85_26510"
     CDS             1048..1410
                     /gene="queF"
                     /locus_tag="VGB85_26510"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEY0137677.1"
                     /translation="MSEHPRPSPILESFPNPRPGRAYEIRFDCPEFTCLCPKTGQPDF
                     ATIRIRYTPGDRCVELKSLKLYLWSYRDQGAFHEAVTNQIVDDLVAVLDPRKLTVEGD
                     FYVRGGIRTVVEVSHPDA"
BASE COUNT          229 a          592 c          605 g          240 t
ORIGIN      
        1 cttccggaca ggtggggcgg cgagcacgtg ccctgggagg acccgcgtcg cagggccccg
       61 cagctcggcg gccccccccg gcccggatag tctcgccccg cgcacctcgc tcacgccaca
      121 cgccgccccg cgcacccggg cacgcgagca cgcgagcacg cgagcacgcg acacgcgagc
      181 acgcgacacg cgacacgtga cacgcgaccc gcgacacgcg acgcattcgc gcgtccggac
      241 cgcgaccttc aatcgatatc gacgccgaac gtgaccgcca tcgcagcccc atcgaaagac
      301 ggaaagcggt ggtcatacac ggcccggcgg gcgcagcttg ggatccccca cacgcggagg
      361 ctggcggggg cggtgaggga cacgctctcg accttgccgc tggggagcac gcgcagcttg
      421 aacttgaggc taccggcgcc gatgtctccg taggcgctcg ccgtggtgac gcagcgctgg
      481 atctgcgggg tcacctgcgc gaagtggcgg tcgagctcgg actcggagag gcgttcgctg
      541 ccggccttca tgtcgagggc ttgcggctcg gcgttgctga attgtgggac gcggctgtcg
      601 tcgaccggga cgaagggggg cggggccgcg gctgtttcct cgacctcgcc ggcagcctcg
      661 gccgcacctg gatctccggc gacggcgggc gtgcctttgc ggccgcgctt gcctctgcgc
      721 ttgcgcttgc cggtcggctc ctcggcgggt ggtggggcgg cgacggcctc gcagcggtcg
      781 gcgacgcact cggtgcccgg gccgcagacg ccgtcgcagg ggaggcggac ctcggtcgta
      841 caggcgacgc agacgcctgc gccgagggcc caggcgaccg gcgcggcacg cagcgctgcg
      901 agcgtacggg gcacggccgc gaaatacgcg ggtggcccgg ggctgtcaag cgaccgcggg
      961 tgcgcccgtg gctcgcgccg gttttcctcg cgcgcgggtg cgggcgagcc agttgcgggg
     1021 ggagcgggct cgtggcagcg tccgccggtg tccgagcacc ccaggccctc gccgatcctc
     1081 gagtccttcc cgaacccgcg cccgggtcgg gcctacgaga tccgcttcga ttgccccgaa
     1141 ttcacgtgtc tgtgccccaa gacgggccag cccgattttg cgacgatccg gatccgctac
     1201 acgccgggcg atcgctgcgt cgagctcaag agcctcaagc tgtacctgtg gtcgtaccgc
     1261 gaccaggggg cgttccacga ggcggtcacc aaccagatcg tcgacgatct ggtcgccgtg
     1321 ctcgacccgc gcaagctgac cgtcgagggc gatttctacg tgcgcggggg gatccggacc
     1381 gtggtcgagg tctcgcaccc ggacgcatga tccgaccgcg gggcccgttt ttcgcccctt
     1441 gtgtggtacc gtgcgcggcg caggcggggg tccgcgtggg ccgctgccgc cggagaccgc
     1501 catgccactg ctgacgagcc tgctgccgaa gtttgcgggc gccctgctca cgctcctcct
     1561 cgacggaccc ttcaattatg agttctcgcg ggtgaccgac gagaagggcc aggcccacct
     1621 cgtattgcgc gccaacgaga acctgccgca gatggaggtc acgatc
//
LOCUS       DASWNJ010001598         1837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_521673, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001598 DASWNJ010000000
VERSION     DASWNJ010001598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521673"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VGB85_26515"
     CDS             <1..240
                     /locus_tag="VGB85_26515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137678.1"
                     /translation="HLLGKQTADLLAVPDERPALATPILCGALAGLDLARASVPALDR
                     YAARAGRRYWDGVVAMGLGGLPADFAPPARLARVP"
     gene            304..1764
                     /locus_tag="VGB85_26520"
     CDS             304..1764
                     /locus_tag="VGB85_26520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137679.1"
                     /translation="MPVPALRISRIISANTVSARPDGEFVCYWMTAARRTTWSYALDH
                     AIARARALGKPLLVLEALRCDYPWASDRLHAFVLAGMRDNAARFAAAGVAYHPYVEPA
                     KGAGKGLLAALAARACQIVADDYPAFFLPRMQAAAASLPVCIERVDGNGILPMRATEI
                     AFPTAYAFRRFIQKKCREHIGERPRADPLARLALPTIPVPKDILARWPAASPQLLACE
                     PAALARLPIDHAVAPCGEGGSKAAGRVLRGFLDARLAAYGERNDPAAASTSGLSPYLH
                     FGQIGAHEVLAGILRREGWTAEALAEGARGGREGWWGVSPAAEGFLDQLLTWRELGFN
                     NAANRPDFTSYESLPPWARRTLEDHAGDRRDPQYTPAQLEAAATHDPLWNAAQRQLIR
                     EGVIHNYLRMLWGKKILEWSASPRVALEVLVHLNNKYALDGRDPNSYSGIFWVLGRHD
                     RPWGPERPVYGKIRYMSSDNTARKLHVREYLAAHGP"
BASE COUNT          251 a          781 c          570 g          235 t
ORIGIN      
        1 cacctgctcg gcaaacaaac cgccgacctg ctcgccgtcc ccgacgagcg acccgccctc
       61 gccacaccga tcctctgcgg cgccctcgct ggcctcgacc tcgcccgcgc cagcgtgccc
      121 gccctcgacc gctacgccgc ccgcgccggc cgccgctact gggatggcgt ggtcgccatg
      181 ggcctcgggg gcctgcccgc cgacttcgcc ccgcccgccc gcctcgcgcg cgtgccctga
      241 cctgtcccga agcacctgtc ccgaagcacc tgtcctccgg gacacagccg aagaaggcct
      301 cgcgtgcccg tccctgcgct ccgcatcagc cgtatcatct ccgccaacac cgtctccgcg
      361 cgccccgacg gcgagttcgt ctgctactgg atgaccgccg cgcggcggac cacgtggagc
      421 tacgcgctcg accacgcgat cgcccgcgcg cgcgcgctcg gcaagcccct gctcgtgctc
      481 gaggccctgc gctgcgatta cccgtgggcc agcgaccgcc tgcacgcctt cgtgctcgcc
      541 ggcatgcgcg acaacgcggc ccgcttcgcc gccgccggcg tcgcctatca cccctacgtc
      601 gagccggcga agggcgcagg caagggcctg ctcgccgcgc tcgccgcgcg cgcctgccag
      661 atcgtcgccg acgattaccc cgccttcttc ctcccgcgga tgcaggccgc cgccgccagc
      721 ctgccggtct gcatcgagcg cgtcgacggc aacggcatcc tccccatgcg cgccaccgag
      781 atcgcgttcc cgaccgccta tgcgtttcgc cgcttcatcc agaagaagtg ccgcgagcac
      841 atcggcgagc gcccgcgggc cgacccgctc gcgcgcctcg cactacccac gatacctgtc
      901 ccgaaggaca tcctcgcgcg ctggcccgcc gcgagcccgc agctgcttgc ctgcgaaccc
      961 gccgcgctcg cccgcctgcc gatcgaccac gccgtcgccc cctgcggcga gggcggcagc
     1021 aaggccgccg gccgggtgct gcgcggcttc ctcgacgccc ggctcgccgc ctacggcgag
     1081 cgcaacgacc cggcagccgc cagcaccagc ggcctctcgc cctacctgca cttcggccag
     1141 atcggcgcgc acgaggtgct cgccggcatc ctccggcgcg agggctggac cgccgaggcg
     1201 ctcgccgagg gcgcccgcgg cggacgtgag ggctggtggg gcgtatcgcc ggccgccgag
     1261 ggcttcctcg accagctgct gacctggcgc gagctcggct tcaacaacgc cgcgaaccgg
     1321 cccgacttca ccagctacga gtccctgccg ccgtgggccc ggcgcacgct cgaggaccac
     1381 gccggcgacc gccgcgaccc gcagtacacg ccggcgcagc tcgaggccgc cgcgactcac
     1441 gacccgctgt ggaacgccgc ccagcgccag ctgatccgcg agggcgtgat ccacaactat
     1501 ctgcggatgt tgtggggcaa gaagatcctc gagtggagcg ccagcccgcg cgtcgcgctc
     1561 gaggtcctcg tgcacctcaa caacaagtat gccctcgacg gccgtgaccc caattcatac
     1621 agcggcatct tctgggtgct cggccgccac gaccgcccct ggggccccga gcggcccgtg
     1681 tacggcaaga tccgctacat gagcagcgac aacaccgcgc gcaagctgca cgtccgcgag
     1741 tacctcgccg cccacggtcc gtgagcgctc accccgagta cgccaccacg agcagtcgcc
     1801 cgtccccggc gagcaccagc gtgcggttcg cgtcgta
//
LOCUS       DASWNJ010001599         7173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_99235, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001599 DASWNJ010000000
VERSION     DASWNJ010001599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7173
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99235"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1443
                     /locus_tag="VGB85_26525"
     CDS             <1..1443
                     /locus_tag="VGB85_26525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137680.1"
                     /translation="LILCSAISGKGQVALAHASVREAVRIRYNDSLNETRLDLAERIA
                     EVDSKDPSLVFLRARLLDDAAESLEAIGELEKAVETLFSAGQPQLAACVLERIILRLR
                     KQGKLPGLPRLLKAQLNMVERAAGALNDPRREAAHFEAGILVAELLGDHRAQALFWLG
                     LVDRYTLEGNVDADVALTRLQQAAVAAKQARDRILELRIANRRAEILLGAGEIDQAVR
                     CSKDAMAILDIPEAQDTHACHIIGTRLRCLSLAGQLAEARRLHDIAKPIAARVSVLQR
                     QSYISGIAYLAVLGNEPERAIPETREAIEQLRAVNITRMLVSPLHNIGDLLLRSGDLE
                     AAGLAFKEALALAGLHGHTYHIHLNAGFLGYTLARLGQVEEGAAMLADARRGLAQIQN
                     EQFAQQQLRLLDAEVAHMVGQSPRARRELEEMLADFQSTSELSLAQWAQEALSRIERD
                     RGTSFIETPEEPESAPASPDEETVRTKPLR"
     gene            1531..3507
                     /locus_tag="VGB85_26530"
     CDS             1531..3507
                     /locus_tag="VGB85_26530"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0137681.1"
                     /translation="MSDAQQIQHDLAHMVGSVLAGTYRLDALIGEGGMGAVFRGRHVL
                     LRRDVAIKVLHPDFARDTELVKRFDREAQSAARLNHPNCVQVTEFGSTDEGMKYLVMQ
                     LLEGTELQNVLDGPTPPERAVDLILQVLRGLEHAHGQGVVHRDLKPQNVFVTRDHEGR
                     EVLKLVDFGIAKIIGGDGALEQMTRAGMVFGTPQYMSPEQALGLEIDARADLYAAGVL
                     LYQIVTGKLPFNSDDPVALIRMQVSTEPPPLPPSVPQELAAIIFRLLAKQKEQRYPDA
                     RTVRIALERYRSTLKPRLNKTGVSEPDPLTDIVASGGDLSGLSAVARPLPDALRPRPM
                     TLVESMMDSDALHRQTWFRVLVGVGVAVLLAGAVAFWTLSGGGETEVKSDPTSGPVTT
                     PAPSTATPATTPGVATPPVPQDRPAGPSEQELRGIDDLLTQDKPEEAATRLQALRAAY
                     PTDAQVRWREAGLQARKPDGGEAALQLYAEALKLDPSLARDLGFHAQVDVLLRKRPVS
                     DAAIDLALALGPQGHGVLVDYINDPKGWATYPQRRKIRDALEKDPAGAAEIDRPLNVR
                     RDLWQFSQSPTPCQTLQAALTAIQAAPPHADNNDALKAVVLPRPGDTADEAERTACVA
                     VVTLYASVRATHAKAFPKAFPKPGKKRPTKKKRR"
     gene            complement(3584..3913)
                     /locus_tag="VGB85_26535"
     CDS             complement(3584..3913)
                     /locus_tag="VGB85_26535"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY0137682.1"
                     /translation="MADRVPDWTFLTNHAHVLFCLAKDPEIRLRDVADRVGITERAVQ
                     RIVTELETAGHITRHRVGRRNTYTVHRQHPLRHPIENHRSVADLLALILHQGEILAPT
                     AKNTETE"
     gene            4059..4655
                     /locus_tag="VGB85_26540"
     CDS             4059..4655
                     /locus_tag="VGB85_26540"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEY0137683.1"
                     /translation="MSRPLVLASTSPYRRKLLEQLDIEFGTCASEFDERAEDARLAEL
                     GIEGFALHLARGKAQSVRSRRPDSWILGADQIAVLPPDELMHKPGTPERAVEQLMRMS
                     GHVLELVTGVVLLDAVTGATHEAVDRQRLTLRAFTRAEAESYVERHRPLDCAGSFRIE
                     DAGITLFERIDSGDFTGIIGLPLLAVCRLLRGVGLLPA"
     gene            5516..>7173
                     /gene="hutU"
                     /locus_tag="VGB85_26545"
     CDS             5516..>7173
                     /gene="hutU"
                     /locus_tag="VGB85_26545"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEY0137684.1"
                     /translation="MQAPTIRAPRGTTLSCKGWQQEAALRMLMNNLDPEVAEDPQNLV
                     VYGGTGKAARSWPDFHRIVASLRDLGNDETLIVQSGRAQGIFKTHEDAPRVLLANSLL
                     VPKWATLAEHQRLEALGLTMFGQMTAGSWIYIGTQGIVQGTYETFAACAREHFGGDLV
                     GRFVLTGGLGGMGGAQPLAATMNGAAILCVEVDPQRIDRRIASGYLMRRSDDLDEALR
                     LIDEARSKGEALSVGLLGNCAEVLPELVRRGIIPDVVTDQTSAHDELHGYVPDGMTLA
                     EAAELRHADPVAYVERSVAAMGKHVAAMRAMQQRGAVAFDYGNNIRAQAVKAGVADAF
                     EIHGFIPLYIRPLFCSGQGPFRWAALSGDPQDIYTTDRAILELFPEKAALRRWIELAQ
                     AQVKFQGLPCRICWLGYGERERAGLLFNELVRTGKVKAPIVIGRDHLDCGSVASPNRE
                     TESMKDGSDAIGDWPVLNALLNTAAGASWVSVHHGGGVGIGYSLHAGMVVVADGTEGA
                     ARRLGRVLVTDPGMGVVRHVDAGYDAALEFAREHDVRVPGRTTL"
BASE COUNT         1132 a         2640 c         2403 g          998 t
ORIGIN      
        1 ctcatcctct gtagcgcgat ctccggcaag gggcaggtcg cgctcgccca cgccagcgtg
       61 cgcgaggccg tgcgcatccg ctacaacgac tcgctcaacg agacccgcct cgacctcgcc
      121 gagcgcatcg ccgaggtcga cagcaaggac ccgagcctgg tgttcctgcg cgcccgcctg
      181 ctcgacgacg ccgccgagtc gctcgaggcg atcggcgagc tcgagaaggc cgtcgagacg
      241 ctgtttagcg ccggccagcc gcaattggcc gcctgcgtgc tcgagcggat catcttgcgc
      301 ctgcgcaagc agggcaagct gcccggcctg ccacgcctgc tcaaggccca gctcaacatg
      361 gtcgagagag cagcgggcgc gctcaacgac ccccgccgcg aagcggccca cttcgaggcc
      421 ggcatcctcg tcgccgagct gctcggggac caccgcgcgc aggcgctgtt ttggctcggg
      481 ctggtcgatc gttacacgtt ggagggcaac gtcgacgccg acgtcgcgct gacgcggctg
      541 cagcaggccg cggtggcggc caagcaggcc cgcgaccgca tcctcgagct gcggatcgcc
      601 aaccgccgcg cggagatcct gctcggcgcc ggcgagatcg accaggccgt gcgctgctcc
      661 aaggacgcga tggcgatcct cgacatcccg gaggcgcagg acacccacgc ctgtcacatc
      721 atcggcaccc gcctgcgctg cctctcgctc gccggacagc tggccgaggc ccggcgcctg
      781 cacgacatcg ccaagccgat cgccgcccgc gtgtccgtgc tgcagcgcca gagctacatc
      841 tccggcatcg cctacctcgc cgtgctgggc aacgagccgg agcgtgcgat ccccgagacc
      901 cgcgaggcga tcgagcagct gcgcgccgtc aacatcacac gcatgctcgt cagcccgttg
      961 cacaacatcg gcgacctgct gctgcgcagc ggcgacctcg aggcggccgg gctcgccttc
     1021 aaggaggccc tcgcgctcgc cggcctgcac gggcacacct atcacatcca cctcaacgcc
     1081 gggttcctcg gctataccct ggcccgcctc ggccaggtcg aggagggcgc cgcgatgctg
     1141 gccgacgcgc gccgcggcct ggcgcagatc caaaacgagc agtttgccca gcagcagctg
     1201 cgcctgctcg acgccgaggt ggcacacatg gtcggacaga gcccgcgcgc gcgccgcgag
     1261 ctcgaggaga tgctggcgga tttccagtcg accagcgagc tgtcgctcgc ccagtgggcg
     1321 caggaggccc tcagccgcat cgaacgcgac cgcggcacgt ccttcatcga gaccccggag
     1381 gagccggagt ccgcgccggc ctcccccgac gaagagaccg tgcgcaccaa gccgcttcgc
     1441 taaaaattat cggccgagcc tgccctgcga tccactccac tgaacttgtc ggccgatctc
     1501 gagtatgttt ccccttaacc tagtcccccg atgagcgacg cgcagcagat ccaacacgat
     1561 ctcgcccata tggtcggcag cgtgctcgcg ggcacctacc ggctcgatgc gttgatcggt
     1621 gagggtggca tgggcgccgt gttccgcggg cgccacgtgc tgctgcgccg cgacgtcgcg
     1681 atcaaggtcc tccacccgga ttttgcccgc gacaccgagc tcgtcaagcg cttcgaccgc
     1741 gaggcccaga gcgccgcccg gctcaaccac cccaactgcg tccaggtgac cgagttcggg
     1801 tcgaccgacg agggcatgaa gtacctcgtg atgcagctgc tcgagggcac cgagctgcaa
     1861 aacgtgctcg acggcccgac cccgcccgag cgggcggtcg acctcatttt acaggtgctc
     1921 cgcggcctcg agcacgcgca cgggcagggc gtggtccacc gcgacctcaa gccgcaaaac
     1981 gtgttcgtca cgcgcgacca cgagggccgc gaggtgctca agctcgtcga cttcggcatc
     2041 gccaagatca tcggcggcga cggtgcgctc gagcagatga cccgcgccgg catggtgttc
     2101 ggcacgccgc agtacatgag cccggagcag gccctcggcc tcgagatcga cgcccgcgcc
     2161 gacctctacg ccgccggcgt gctgctgtac cagatcgtca ccggcaagct gcccttcaac
     2221 agcgacgacc cggtcgcgct gatccgcatg caggtcagca ccgagccgcc gccgctgccg
     2281 ccctcggtcc cgcaggagct cgccgcgatc atctttcgcc tgctcgccaa gcaaaaagag
     2341 cagcgctacc ccgacgcccg caccgtccgc atcgcgctcg agcgctaccg cagcacgctc
     2401 aagccgcgcc tcaacaagac cggcgtctcc gagcccgacc ccctcaccga catcgtcgcc
     2461 agcggcggcg acctctccgg cctcagcgcc gtcgcccgcc cgctgcccga cgccctgcgc
     2521 ccgcgcccga tgaccctcgt cgagtcgatg atggacagcg acgcgctgca ccgccagacc
     2581 tggttccgcg tgctcgtcgg ggtcggcgtg gccgtcctgc tcgccggcgc cgtcgcgttc
     2641 tggacgctct ccggcggcgg cgagaccgag gtgaagtcgg acccgacgag cggaccggtc
     2701 accacgccgg ccccgtccac cgccacgcca gcgaccaccc ccggggtcgc cacgccacct
     2761 gtcccgcagg acaggcccgc cggaccctcg gagcaggagc tgcgcggcat cgacgacctg
     2821 ctgacgcagg acaagcccga ggaggccgcc acgcgcctgc aggcgctgcg cgccgcctat
     2881 ccgaccgacg cgcaggtgcg ctggcgcgag gccggcctgc aggcgcgcaa gcccgacggc
     2941 ggcgaggcgg cgctgcagct gtacgccgag gcgctcaagc tcgacccctc gctggcgcgc
     3001 gacctcggct tccacgcgca ggtcgatgtc ctgctgcgca agcgtccggt cagcgacgcc
     3061 gcgatcgacc tggcgctggc gctcggcccc cagggccacg gcgtcctcgt cgactacatc
     3121 aatgacccca agggctgggc gacctacccc cagcgccgca agatccgcga cgcgcttgaa
     3181 aaggaccccg ccggcgccgc cgagatcgat cggccgctca acgtccgtcg cgacctgtgg
     3241 cagttctcgc agtcgccgac gccgtgccag accctccagg ccgcgctcac tgcgatccag
     3301 gccgccccgc cgcacgccga caacaacgac gcgctcaagg ccgtcgtgct gccgaggccg
     3361 ggcgacacgg ccgacgaggc cgagcgcacc gcgtgcgtcg ccgtcgtgac gctgtacgcc
     3421 agcgtgcgcg cgacgcatgc gaaggcgttc ccgaaggcgt tcccgaagcc gggcaagaag
     3481 cgcccgacca agaagaagcg ccgctagtcg gcgcggctgc tagtcggcgc ggctgctagt
     3541 cggcgcggct gcaggtcggc gcggctgcag gtcggcgcgg cgactactcg gtttcggtgt
     3601 tcttggccgt gggggcgagg atctcgccct gatgcaggat gagcgccagc aggtcggcga
     3661 cgctgcggtg attctcgatc gggtgccgca gcgggtgctg gcgatgcacc gtataggtat
     3721 tgcgccggcc gacgcgatgc cgggtgatgt gccccgcggt ctcgagctcc gtcacgatcc
     3781 tctggaccgc gcgctccgtg atgccgaccc gatccgcgac gtcacggaga cggatctccg
     3841 gatctttggc gaggcagaac agcacgtggg cgtgattggt caggaaggtc cagtcaggga
     3901 ctcgatcggc cactgggcga ggctccaagg gtgcaggtaa gttcaaaggg tccaggtctg
     3961 gaccgcgcgg cgtgcagaaa tgcattacct tgtcaagcgc atgatgcgcg gctcgcggcg
     4021 gatggtcaag cgtctacagc tcttctaagc tcgctccaat gtcgcgtcca ctggtgctcg
     4081 cctcgacatc gccctatcgg cgaaagctgc tcgagcagct tgacatagaa ttcgggactt
     4141 gcgcttcaga gttcgacgag cgggccgagg atgctcgcct cgccgagctg gggatcgaag
     4201 gttttgcgct gcatctggcg cgcggcaagg cccaaagcgt gcgatcacgc cgacccgaca
     4261 gctggatcct cggcgcggac cagatcgccg tgctcccgcc cgatgaattg atgcacaagc
     4321 caggcacccc cgagcgggcc gtcgaacagc tgatgcgcat gtcgggccat gtgctcgagc
     4381 tcgtgaccgg ggtcgtgctg ctcgacgcgg tcaccggcgc gacccacgag gcggtcgacc
     4441 gccagcggct gacgctccgc gccttcacgc gcgccgaggc cgagtcctac gtcgagcgcc
     4501 accgaccgct cgactgcgcc ggcagcttcc gcatcgagga cgccggcatc accctgttcg
     4561 aacgcatcga cagcggcgac ttcacgggga tcatcggact gccgctgctc gcggtgtgcc
     4621 gcctgctgcg cggcgtcggt ctgctgccgg cctgagtgcc acccgaccgg gcccagagtc
     4681 acacgccggc cgccccagcg gccccggagc cgcatgacgg gccccttatg gagacatacc
     4741 gacgaccact cggggacgtt cacggggtcg ggcggcctca ccgagtccag cggcaacaag
     4801 gacttcggca gcgcctgggg caccgatgcg agcaccggcg acgccgcatg cacgctcgct
     4861 gccagacggc gcactgcggc gacggcctcg tgcagacgga gatcgaagcc tgtgtgaagg
     4921 ccagctgcgg cgacggccag gtgcaggagg gcgtcgagac ctgcgacgac gccctgccgc
     4981 agcgctgcaa gcagatcaaa gacgcgcagc cggcggccga ggacggcatc tacacagcgt
     5041 ctgcgccccg ctactcgccg ccactccaac gtcgcgtgat cgatgcacct gcatcgcttc
     5101 ggtgctttgc tggatcgatg acgatattgt gcgcagtgcc ggctcacagc gatagagaca
     5161 ggggcggtga aagcaacatg ttcttccgga caggtggggc ggcgagcacg tgccctggga
     5221 ggaccgcgtc gcagggaccc gcagtccgcc ggcccccccg gcccatgtag cctcgccccg
     5281 cgttactcga gcacgccctg tccgcgacgc gagcacaccg tccgcgcgac gcgagcacac
     5341 cgcccgcgcg acgcgagctc gtcgcgcgac gcgagccagc cacccgcgcg agcgagcagc
     5401 ccccgcgagc ccgccgcctg cgcgaccggg cgagcccgcg gcagccaaaa cacgcgtgaa
     5461 acatcacgcg gccgcgagcg gcggcggccc ccctcgcggc tacgatcgcc cggccatgca
     5521 agctccgacg atccgcgccc cccgaggtac gaccctgtct tgcaagggat ggcagcaaga
     5581 ggccgccctg cgcatgctga tgaacaacct cgatcccgag gtcgcggagg acccgcagaa
     5641 cctggtcgtg tatggcggca ccggcaaggc ggcccgttcg tggcccgact ttcaccgcat
     5701 cgtcgccagc ttgcgtgacc tcggcaacga cgagactctg atcgtccaga gcggccgcgc
     5761 gcagggaatc ttcaagaccc acgaggacgc gccgcgggtg ctcctcgcca acagcttgct
     5821 ggtgccgaag tgggcgacgc tcgccgagca tcagcggctc gaggcgctcg gcctgacgat
     5881 gttcgggcag atgacggcgg gctcgtggat ctacatcggc acgcagggca ttgttcaagg
     5941 cacctacgag acctttgccg cgtgcgcgcg cgagcacttt ggtggcgacc tcgtcggccg
     6001 gttcgtgttg acgggcgggc tcggcgggat gggcggcgcg cagccgctgg cggcgacgat
     6061 gaacggcgcg gcgatccttt gcgtcgaggt cgacccgcag cgcatcgatc gccggatcgc
     6121 ctcgggatat ctcatgcggc gcagcgacga cctcgacgag gcgctgcgcc tgatcgacga
     6181 ggcgcgcagc aagggtgagg cgctcagcgt cgggctgctc ggcaactgcg cggaggtcct
     6241 gccggagctg gtgcggcgcg gcatcatccc cgatgtcgtc accgaccaga cctcggcgca
     6301 cgacgagctg cacggctacg tgcccgatgg catgaccctc gccgaggccg cggagctgcg
     6361 ccacgccgat ccggtcgcgt acgtcgagcg cagcgtcgcg gcgatgggca agcacgtcgc
     6421 ggcgatgcga gcgatgcagc agcgcggcgc tgtcgccttc gattacggca ataacatccg
     6481 cgcgcaggcg gtcaaggccg gggtcgccga cgccttcgag atccatggct tcattccgct
     6541 gtacatccgc ccgctgtttt gcagcggcca gggcccgttt cgctgggccg cgctgtcggg
     6601 tgacccgcag gacatctata cgaccgaccg cgcgatcctc gaactgttcc ccgagaaagc
     6661 cgcgctgcgt cgctggatcg agctggcgca ggcgcaggtc aagttccagg gcctgccgtg
     6721 ccggatctgc tggctcggct acggcgagcg cgagcgcgcc ggcctgctgt tcaatgagct
     6781 cgtgcggacc ggcaaggtca aggcgccgat cgtcatcggc cgtgaccacc tcgactgtgg
     6841 cagcgtcgcc tcgcccaacc gcgagaccga gtccatgaag gacggctccg acgcgatcgg
     6901 tgactggccg gtcctcaatg ccctgctcaa caccgccgcc ggcgcgtcgt gggtgtccgt
     6961 gcatcacggc ggcggcgtcg gcatcggcta ctcgctgcac gccggtatgg tcgtcgtcgc
     7021 cgacggcacc gagggcgccg cgcggcgcct cggccgtgtg ctcgtcaccg accccggcat
     7081 gggcgtcgtc cgccacgtcg acgccggtta tgacgccgcc ctcgagttcg cccgcgaaca
     7141 cgacgtgcgc gtgcccggcc gcaccacgct gat
//
LOCUS       DASWNJ010001600         5740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_698112, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001600 DASWNJ010000000
VERSION     DASWNJ010001600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698112"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="VGB85_26550"
     CDS             <1..976
                     /locus_tag="VGB85_26550"
                     /inference="COORDINATES: protein motif:HMM:NF024985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HEY0137685.1"
                     /translation="AASFGGGGGFEGSAASAASSSGMMFQEAEHEVAPEQLAYGDLRM
                     PGPLDSRRGSLIVSQRRDRYLEMLWVREVTREIDVVALIDGAINLAQRAGAGGPPPRH
                     TLAHSQGGFDYVYAADSVVDIPSDGAFHGIPLITRSATIELGYIVVPRESKDVFRHVK
                     LKNPLDAPLLPGPADIYVGGDYLLSTDLRLAAPRGEVKIGLGVEQAIKVSRNTSYAEE
                     AAGLMGGSLTLRHEIVIEVANRRDRPVNLDVLERLPTLREREDEIALEVAVVDPPWSA
                     YDPEEYALKGGHRWQVQVPAGQQKKLRVVYVIKMSAKKELVGGNRREQ"
     gene            980..2617
                     /locus_tag="VGB85_26555"
     CDS             980..2617
                     /locus_tag="VGB85_26555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HEY0137686.1"
                     /translation="MPEPTIVDKPSGDLASLSAPILDLKAPVVEVTVLEDRAHVVRRG
                     VIEIFGGMTRLRVTGVSPVLADKTLVAAVVKVGQKTGDASRERINDVRVKRSRLVLGE
                     DKPEQRRALEQEHEQEQRKLDLMHARLTQLQIQQQSLEAVADVTVTDIGVDVSHGQSN
                     PDAWRMQLEQIANQEEGIRKQRVELEFEIREQEALLKRLRRRMSATAQVSDSAAAEVI
                     VEVWAAAAGIYSVQVDYIVPGACWRPYHRAQLVELVKGKEPVLHFASEGCVWQNTGED
                     WNDVQFIFSTERPSLGMEPPQLASDVLYAQRKSEVLVVEAREQEVQTTGLGGAPKQRT
                     ADLPGIDDGGEALKLRSTSKASVLSDGRPHRVPLMAFTSPTSIEHVLMPELAEAVVLK
                     STHVNRSQLPILAGPVDLVREGGFVGRSSLLFIAPGENFAVGWGPDGALRVQRTVELS
                     KEDRGAMSSWTTQAHLVKVRISNLGPQERAIHVTERVPVSEIEKLRIEVDAAGTSDKQ
                     RPDNNGFVHWKFDLPAFGRRTVELKYIVRKHADVVGF"
     gene            2722..3597
                     /locus_tag="VGB85_26560"
     CDS             2722..3597
                     /locus_tag="VGB85_26560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0137687.1"
                     /translation="MDDREQLLAELAALRADNEHLRCELEEADAASEKFRVLFEYSSD
                     AHLIFDDRGIIDCNNAAVKMLRCADKHHVLRLHPATLSPEIQPDGRHSLEKSVEMDRV
                     ARERGYHRFEWMHRRMTGEDFPVEVTLTPVVLSTGPALLVVWHELTEIRQREFELREQ
                     LELIRTQQAEIRRLSMPILEVWDGVLLIPVLGTLDHEAARALAGPALAAIGRARARTV
                     IIDLTGLTDADAATARHLVELLTAIQLLGASGIVVGIGPSVALAFLTADAGLSRVRTR
                     ASLRQALLLCLRPGA"
     gene            complement(3631..5298)
                     /locus_tag="VGB85_26565"
     CDS             complement(3631..5298)
                     /locus_tag="VGB85_26565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137688.1"
                     /translation="MLPCSPRLASLTILALICPACGDDNAGESASAETVTGESLSSAE
                     TSMSSEPPTTGEGTTTPDPDTTTGEPEPAPFVPGPVRGGLEIDWVEANQGVGVAIGRD
                     GAGVGGAERTSYLLQNRLTLIRAFWKPLPADWVPRKIEGRLTIKYADNTEIVLKSKAT
                     MVDQDSFIGSLDRSFFWGLMKEQVVPGMKYNIELFEREPGHDDLPAGANPPRLPHTGD
                     TFVGIEQSDQVMKVTLVPFIYDSGDGCKTTPDTSEATMKVFQDQMFMMNPLDTLDFTL
                     HEPVAWNTKLNDFNELNEFMSGMRADEGAEPERYYYGLVDVCSGGLGGAGGKAYGIPQ
                     GGEMGDAWQRVSSGLSDTLDFSSETFVHEVGHSQGRYHVDCGGAQGFDPNYPWEGGEI
                     HDWGFGIVNFKLYHPTVHKDYMTYCHPVWASSWGWNKVYPIIKTLSSWDNAGAPNPDD
                     VVGSILVGSIYPDGRETWITVPGNVNARDISAVHSVEFKIDGETVQQPAAFLTQPEGD
                     VVNVVTAVPDRWDNVTEMIRIAGPKRIGVPIAKIRQHHRPKPISAAH"
     gene            complement(5354..>5740)
                     /locus_tag="VGB85_26570"
     CDS             complement(5354..>5740)
                     /locus_tag="VGB85_26570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137689.1"
                     /translation="DPHPGEVLLTGMQGPYLDQDGAGTLVRGLARSLGPTAAAVGAGA
                     LVLLALAGLLHWLRAGLRSSGQLLGGQTTVGFGLVFLVCAALGPALELTPLLRITDAA
                     AALAVLLGTARLLAYLPRLVRRDRDA"
BASE COUNT          886 a         2036 c         1991 g          827 t
ORIGIN      
        1 ggccgccagc ttcggcggcg gcggcggctt cgagggctcc gcggcctccg cggcctcgtc
       61 gagcgggatg atgttccagg aggccgagca cgaggtcgcg ccggagcagc tcgcgtacgg
      121 cgacctgcgc atgcccggcc cgctcgacag ccgccgcggc tcgctgatcg tcagccagcg
      181 ccgcgaccgc tacctcgaga tgttgtgggt ccgcgaggtg acccgcgaga tcgacgtcgt
      241 cgccctgatc gacggcgcga tcaacctcgc ccagcgggcc ggcgccggcg gaccgccgcc
      301 gcgccacacc ctcgcgcact cgcagggcgg cttcgactac gtgtacgccg ccgacagcgt
      361 cgtcgacatc ccgtccgacg gcgcgtttca tggcatcccg ctgatcaccc gcagcgcgac
      421 gatcgagctc ggctacatcg tcgtgccgcg cgagagcaag gacgtgttcc gccacgtcaa
      481 gctcaagaac ccgctcgacg ccccgctgct gcccggcccg gccgacatct acgtcggcgg
      541 cgactacctg ctgagcaccg acctccggct cgccgccccg cgcggcgagg tgaagatcgg
      601 cctcggcgtc gagcaggcga tcaaggtctc gcgcaacacc agctacgccg aggaagccgc
      661 gggtctgatg ggcggctcgc tcacgctgcg ccacgagatc gtcatcgagg tcgccaaccg
      721 ccgcgaccgg ccggtcaacc tcgacgtgct cgagcgcctg ccgacgctgc gcgagcgcga
      781 ggacgagatc gcgctcgagg tcgccgtcgt cgacccgccg tggagcgcct acgaccccga
      841 ggagtacgcg ctcaagggcg gccatcgctg gcaggtccag gtgcccgcgg gccagcagaa
      901 gaagctgcgc gtcgtctacg tgatcaagat gtccgccaag aaggaactcg tcggcggcaa
      961 ccgccgggag caatgagcca tgcctgagcc cacgatcgtc gacaaaccct ccggggacct
     1021 ggccagcctc agcgcgccga tcctcgacct caaggccccc gtcgtcgagg tcaccgtgct
     1081 cgaggaccgc gcccacgtgg tccgccgcgg cgtgatcgag atcttcggcg gcatgacccg
     1141 cctgcgcgtc accggcgtgt cgcccgtgct cgccgacaag accctcgtcg ccgcggtcgt
     1201 caaggtcggt cagaagaccg gcgacgcgtc gcgcgagcgc atcaacgacg tccgggtcaa
     1261 gcgctcgcgc ctggtgctcg gcgaggacaa gccggagcag cgccgcgcgc tcgagcagga
     1321 gcacgagcag gagcagcgaa agctcgacct catgcacgcc cgcctcacgc agctgcagat
     1381 ccagcagcag agcctcgagg ccgtcgccga cgtcaccgtc accgacatcg gcgtcgacgt
     1441 cagccacggc cagtcgaacc ccgacgcgtg gcgcatgcag ctcgagcaga tcgccaacca
     1501 ggaggagggg atccgcaagc agcgcgtcga gctcgagttc gagatccgcg agcaggaggc
     1561 gctcctcaag cgcctgcgcc gacgcatgtc cgccaccgcc caggtcagcg actccgcggc
     1621 cgcggaggtc atcgtcgagg tgtgggccgc cgccgccggc atctactccg tccaggtcga
     1681 ctacatcgtg cccggcgcct gctggcggcc ataccaccgc gcccagctgg tcgagctcgt
     1741 caagggcaag gagcccgtgc tgcacttcgc cagcgaaggc tgcgtgtggc agaacaccgg
     1801 cgaggactgg aacgacgtcc agttcatctt ctcgaccgag cgcccctcgc tcggcatgga
     1861 gccgccgcag ctcgccagcg acgtgctgta cgcccagcgc aagagcgagg tgctggtggt
     1921 cgaggcgcgc gagcaggagg tccagacgac gggcctcggc ggcgcgccca agcagcgcac
     1981 cgcggacctg cccggcatcg acgacggcgg ggaggcgctc aagttgaggt cgacgagcaa
     2041 ggcgagcgtg ctgagcgacg gccggccgca ccgcgtgccg ctgatggcgt tcacctcacc
     2101 gacgagcatc gagcacgtat tgatgccgga gctcgcggag gcggtggtgc tcaagagcac
     2161 ccacgtcaac cgctcgcagc tgccgatcct cgccggcccg gtcgacctcg tgcgcgaggg
     2221 cgggttcgtc ggtcgcagct cgctgctgtt catcgccccg ggcgagaact tcgccgtcgg
     2281 ctggggcccg gacggggcgc tccgcgtcca gcggaccgtc gagctgtcca aggaggaccg
     2341 cggcgcgatg agctcgtgga cgacccaggc gcacctggtc aaggtccgga tctccaacct
     2401 cggcccgcag gagcgggcga tccacgtcac cgaacgcgtg cccgtctcgg agatcgagaa
     2461 gctgcgcatc gaggtcgacg ccgccggcac cagcgacaaa caaaggcccg acaacaacgg
     2521 cttcgtgcac tggaagttcg acctcccggc cttcggccgc cgcacggtcg agctcaagta
     2581 catcgtgcgc aagcacgcag acgtggtcgg attctgatcg ggtcacacgt ccgttcagcg
     2641 caggatcgcc cgcgcgtcgc gcgagcgtga tgcgccctgg aacgttgtgt acgggcgggt
     2701 gcgctgcgat gatgactcgc cgtggacgat cgagagcaac tgctcgccga gctcgccgcc
     2761 ctgcgggcgg acaacgagca cctcaggtgc gagctcgagg aggccgacgc cgcgagcgaa
     2821 aaatttcgcg tgctcttcga gtactcgtcg gacgcacacc tgatcttcga cgaccgcggc
     2881 atcatcgact gcaacaacgc cgcggtgaag atgctgcgct gcgccgacaa gcatcacgtg
     2941 ctgcgcctgc accccgcgac gctgtcgccc gagatccagc cggacggtcg acactcgctc
     3001 gagaagagcg tcgagatgga ccgcgtcgcg cgtgagcgcg gctatcaccg cttcgagtgg
     3061 atgcaccgcc gcatgaccgg cgaggacttc ccggtcgagg tcacgctgac cccggtcgtg
     3121 ctctcgaccg gcccggcgct cctcgtggtc tggcacgagc tgaccgagat ccggcagcga
     3181 gagttcgagc tgcgcgagca gctggagctc atccgcaccc agcaggccga gatccgccgc
     3241 ctctcgatgc cgatcctcga ggtctgggac ggcgtgctgc tgatccccgt gctcggcacg
     3301 ctcgaccacg aggccgctcg cgccctcgcc gggccggccc tcgctgcgat cggccgcgcc
     3361 cgcgcgcgca ccgtcatcat cgatctcacc gggctcaccg acgccgacgc ggcgaccgcc
     3421 cgccacctcg tcgagctgct gacggcgatc cagctgctcg gcgcctccgg catcgtcgtc
     3481 ggcatcggac cctccgtcgc gctcgccttc ctgaccgcgg acgccggcct gtcgcgcgtg
     3541 cgcacgcggg cgtccttgcg ccaggccctg ctgctgtgcc tgcgccccgg cgcttgagcg
     3601 cgacgcgtgc ccgccgaacg ggcgagcagc tcagtgagcc gccgagatcg gcttcggccg
     3661 gtgatgctgc cggatcttcg cgatcggcac gccgatccgc ttgggcccgg cgatacggat
     3721 catctcggtg acattgtccc agcgatcggg cacggcggtg acgacgttga cgacatcacc
     3781 ctcgggctgg gtcaggaagg ccgcgggctg ctggacggtc tcgccgtcga tcttgaactc
     3841 gacgctgtgc accgcgctga tgtccctggc gttgacgttg ccgggcaccg taatccaggt
     3901 ctcgcggccg tcggggtaga tcgatccgac gaggatcgaa ccgacgacgt cgtcggggtt
     3961 gggggcgccg gcgttgtccc agctgctgag ggtcttgatg atcgggtaga ccttgttcca
     4021 gccccagcta gacgcccaga ccgggtggca gtaggtcatg tagtccttgt gcacggtcgg
     4081 gtggtagagc ttgaagttga cgatgccaaa gccccagtcg tggatctcgc cgccctccca
     4141 cgggtagttc gggtcgaagc cctgcgcgcc gccgcagtcg acgtggtagc ggccctgcga
     4201 gtggccgacc tcgtgcacga aggtctcgct cgagaagtcg agggtgtccg acagaccgga
     4261 ggagacgcgc tgccaggcgt cgcccatctc gccgccctgc gggatgccgt aggccttgcc
     4321 gcccgcgccg ccgaggccgc cgctgcagac atcgaccagg ccgtagtaat agcgctcggg
     4381 ctcggcgccc tcgtcggccc gcatcccgct catgaactcg ttgagctcgt tgaagtcgtt
     4441 gagcttggtg ttccaggcga ccggctcgtg gagcgtgaag tccagcgtgt cgagcgggtt
     4501 catcatgaac atctgatcct ggaagacctt catcgtcgcc tcggacgtgt cgggggtcgt
     4561 cttgcagccg tcgcccgagt cgtagataaa gggcacgagc gtcaccttca tcacctggtc
     4621 gctctgctcg atgccgacga aggtgtcgcc ggtgtggggc aagcgcggcg ggttggcgcc
     4681 ggccgggagg tcgtcgtggc cgggctcgcg ctcgaagagc tcgatgttgt atttcatccc
     4741 gggcacgacc tgctccttca tcaggcccca gaagaacgag cggtcgaggc tgccgatgaa
     4801 cgagtcctgg tcgaccatgg tcgccttgga cttgaggacg atctcggtgt tgtcggcgta
     4861 cttgatggtc agccggcctt cgatcttgcg cgggacccag tccgcgggca gcggcttcca
     4921 gaacgcgcgg atcagggtca ggcggttctg cagcaggtac gaggtgcgct cggctccgcc
     4981 gacccccgcg ccgtcgcggc cgatcgccac gccgacgccc tggttggcct cgacccagtc
     5041 gatctcgagg ccgccacgca cggggcccgg cacgaacggc gccggctcgg gctcgcccgt
     5101 ggtcgtatcg gggtcgggag tcgtcgtgcc ttcgcccgtg gtgggcggct cggacgacat
     5161 gctcgtctcg gcgctcgaga ggctctcgcc ggtgaccgtc tcggcgctcg cggactcgcc
     5221 cgcgttgtcg tcgccgcagg cgggacagat gagggccagg atcgtgagcg aggcgaggcg
     5281 aggcgagcag ggcagcatga caaaaaccct acccctggat cccgcccagc cacaagcggc
     5341 cgcgccacac gtttcaggcg tcgcggtctc gccggaccag gcgcggcaga tacgcgagca
     5401 gccgcgctgt ccccaggagc accgccagcg ccgcggccgc atcggtgata cgcagcagcg
     5461 gtgtcagctc cagagctggc ccgagcgccg cgcagaccag gaacaccagg ccgaagccga
     5521 ccgtggtctg gccgccgagc agctggccgg aggaccgcag gccggcgcgt agccagtgga
     5581 gcaggccggc gagcgccagc aggaccagcg cgcccgcgcc gacggcggcg gcggtcgggc
     5641 cgaggctccg ggcgaggccc cgtaccaggg tgccggcgcc gtcctggtcg agatacgggc
     5701 cctgcatgcc ggtcaggagg acttccccgg ggtgcgggtc
//
LOCUS       DASWNJ010001601         5068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_380074, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001601 DASWNJ010000000
VERSION     DASWNJ010001601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380074"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VGB85_26575"
     CDS             <1..680
                     /locus_tag="VGB85_26575"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0137690.1"
                     /translation="ARSLGPTLAYAVPGFVLATTSALLGGFAAARWRGWPGRALLATS
                     TVLMSTSSVIIVVLGQYLLAHRLRVFPVLGWPLAGDARELAGFVILPALLWAVIQLGP
                     DLRHYRAVFVRELAAPHLEGLRARGLGEVRIARHVLRGAVAPVLARISARLPQIVLGS
                     VVVEHVFNVPGVGALLIAAIQDGDLAVVQGIAFCLAAATIVGQALCDVAVYFLDPRTR
                     ARAEAGR"
     gene            677..1486
                     /locus_tag="VGB85_26580"
     CDS             677..1486
                     /locus_tag="VGB85_26580"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0137691.1"
                     /translation="MRRPGELGVTGTLALAGLAGFAVLATGAALGLYAQNFEQAVARE
                     HMPPGPGAWLGTDALGRDLLARTIQGARVSLAVGVGAALGMVSVGTLLGALAGMRRGW
                     TDRALSSLTDAVAAIPPVVLLLAAALILTPGMLTAIVAISLTQWTGVFRSVRAEALRM
                     RSADHDRAAVAMAASTGHRVRWHLIPRLRPLLVTSLVLCFVYAVKVEAVLAYFGASAR
                     LPSWGRLLAESGSELARGIWWPIAAASVPLALLVLCAQLLADRLTDDEPGG"
     gene            1633..2997
                     /locus_tag="VGB85_26585"
     CDS             1633..2997
                     /locus_tag="VGB85_26585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137692.1"
                     /translation="MDSSPAGTSGDPGSTSTSTTGVTTDASATPTTTDEPVTTATTSA
                     TTTTDEPDATTGSIPGWPLPEEQPAPAEGGGVSDPPTNTLHNHMCAGVDWQRIHGWLL
                     LPHAAPELGPPGEIERCVERYAGWVTNEADAAMVSRGSVYAALAASGQCDEGGDYTGT
                     MVTGAWCAAVNPGYGEAECLAQMAKLRSFGVRTLAQVIGHPDAVAAHAQDIPLMAAFV
                     GSGEVACGGDDRWKLAAPEGYVDRYVAAYNAYKAQSSELPACSKRIVVSVALYTGLDT
                     PGVDGVTGANGCWTYERVAKQNEEWKICNFDGTISHANGNKWAYDDTSTNHNSNTEKA
                     GILACQQDVPGRGYVYMTNRGSGWPKVVTEGVEVHFAEIYSGQFAVDDQFNLWKGEGA
                     PGEPMVNFGEPSTGAATIAKATQRSCDEVEDGGYLGVYVYPESLRAARMSAMVEALNT
                     CTGL"
     gene            3254..4003
                     /locus_tag="VGB85_26590"
     CDS             3254..4003
                     /locus_tag="VGB85_26590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137693.1"
                     /translation="MNEFLSAILSFPTVVFTVLLGVIVLYWLMVIVGAIGVDVLHVGE
                     AAGGKIEGIVDGAVEGKIEGVVDGAVSGHAEGATGKLEAVGGGGGFLAMLGFGKVPAT
                     VVISSLVLWSWALCLLIARSGAEMGLPSWSLQVLAPVLAVFLGGVLTGALVRGIGRFI
                     VHQEPLRRADLVGKLCTITTGRVDANFGQALISEHGVDLTVDVRCDPPNSLRRNAQAV
                     LVAYDSEHHFFTVEAYDQPLATLGERPPAAH"
     gene            4024..>5068
                     /locus_tag="VGB85_26595"
     CDS             4024..>5068
                     /locus_tag="VGB85_26595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY0137694.1"
                     /translation="MLESQFVVAAGLEEIGLPLAAVIAGVIFLVIVGTFFIFSRFYRQ
                     VDQGKALIVNKKSGPVVVFTGSVVLPIVHRAEIMDISVKSLDIDRRGKDGLICKDNIR
                     ADIKVAFFVRVNKTPEDVLKVAQSIGCARASDLETLDELFSAKFSEALKTVGKQLEFE
                     ELYTKRGEFKDQLIEVIGRDLNGYILDDAAIDYLEQTPLNSLDPHNILDAQGIRKITE
                     ITAKQNVFTNELRQKERMDVGSQNLTADEAVFRYEQQRADAEAKKHKEIAISMAREQN
                     EAMRVASDENKKTSLVQQINEGEIGKAEQDKVRQIAIAEKAREREISIEVERNEKARA
                     TEAIGRERAVELLR"
BASE COUNT          755 a         1688 c         1842 g          783 t
ORIGIN      
        1 tcgcgcgctc gctcgggccg acgctcgcgt acgccgtgcc gggcttcgtg ctggcgacca
       61 cgagcgcgct gctcggcggc ttcgcggcgg cgcgctggcg cggctggccg ggtcgcgcgc
      121 tgctggcgac ctcgaccgtg ctgatgagca cctcgagcgt gatcatcgtc gtgctcgggc
      181 aatacctgct ggcccaccga ttacgcgtgt ttccggtgct cggctggccg ctcgccggcg
      241 atgcccgcga gctcgccggc ttcgtgatcc tgccggcgct gctgtgggcg gtgatccagc
      301 tcgggcccga cctgcggcac taccgggccg tgttcgtgcg cgagctggcc gcgccacacc
      361 tcgagggcct gcgcgcgcgc gggctcggcg aggtgcggat cgcccgtcac gtgctgcgcg
      421 gggcggtcgc gccggtgctc gcgcggatct cggcccggct gccgcagatc gtgctcggca
      481 gcgtggtcgt cgagcacgtg ttcaacgtgc ccggcgtcgg tgcattgctg atcgccgcga
      541 tccaggacgg tgacctcgcg gtcgtgcagg gcatcgcgtt ttgcctcgcc gcggcgacga
      601 tcgtcgggca ggcgctgtgc gatgtcgccg tgtattttct cgacccgcgc acgcgggcgc
      661 gcgcggaggc cggccgatga ggcgccccgg cgagctcggg gtcaccggca cgctggccct
      721 cgccggcctc gcgggcttcg ccgtgctggc gaccggcgcg gcgctcggcc tgtacgcgca
      781 gaacttcgag caggcggtgg cccgcgagca catgcccccg gggccaggtg cgtggctcgg
      841 caccgacgcg ctcgggcgtg acctgctggc gcgaacgatc cagggggccc gcgtgtcgct
      901 cgcggtcggg gtcggcgcgg cgctcgggat ggtcagcgtc ggtacgctgc tcggcgcgct
      961 cgccgggatg cgccgagggt ggaccgatcg ggcgctgtcg agcctcaccg atgcggtcgc
     1021 cgcgatcccc ccggtggtcc tgctgctcgc ggcggcgttg atcctgacgc cgggcatgct
     1081 gacggcgatc gttgcgatct cgctgacgca gtggaccggc gtgttccgca gcgtccgcgc
     1141 ggaggccctg cgcatgcgct cggccgacca cgaccgtgcc gcggtggcga tggccgcgag
     1201 caccgggcac cgcgtccgct ggcacctgat cccgcggttg cgcccgctgc tcgtcacctc
     1261 gctggtgctg tgttttgttt atgcggtcaa ggtcgaggcc gtgctggcgt acttcggggc
     1321 cagcgcgcgg ctgcccagct ggggccgcct gctcgccgag agcggcagcg agctggcacg
     1381 cggcatctgg tggccgatcg ccgccgcgag cgtgcccctg gcgctgctgg tactgtgcgc
     1441 gcagctgctg gccgatcgcc tgaccgacga cgagcccggc ggatgaagaa gcgctggtcc
     1501 gcgcgcctgc gatcggcgaa cgtgggggtc atgcgtcgcg tcaccccgtc cccgagctcg
     1561 cgccagctcc ccctcgcgtg tggactgctg cttgtcgcct cgatcgctgg ctgtcccgcg
     1621 agcggcggcg atatggattc gagcccggcc gggaccagcg gtgatcccgg cagcacctcg
     1681 acctcgacca cgggcgtgac cacggacgcg agcgccacgc cgacgaccac ggacgagccg
     1741 gtgaccaccg cgaccacgtc ggcgacgaca acgaccgacg agcctgacgc gacgactggc
     1801 agcatccccg gctggccgct gccggaggag cagccggcgc cggccgaggg cggcggtgtc
     1861 tcggatcctc cgaccaacac cctgcacaat cacatgtgtg cgggcgttga ctggcagcgg
     1921 atccacgggt ggctgttgtt gccccacgcc gcgcccgagc tcggaccacc cggtgagatc
     1981 gagcgctgcg tcgagcgtta cgccggctgg gtcacgaacg aggccgacgc ggcgatggtc
     2041 tcgcgcgggt ccgtgtacgc cgcgctcgcg gcgagcggac agtgcgacga ggggggagat
     2101 tataccggca cgatggtcac cggggcgtgg tgcgccgcgg tcaaccccgg ctacggtgag
     2161 gccgagtgcc tggcccagat ggccaagctg cgcagcttcg gggttcgcac gctcgcgcag
     2221 gtgatcggcc accccgacgc ggtcgccgca cacgcgcagg acatcccgtt gatggccgcg
     2281 ttcgtcggca gcggcgaggt cgcctgcggc ggcgacgacc gctggaagct cgccgcgccc
     2341 gaaggttacg tcgatcgtta cgtcgccgcc tacaacgcat acaaggcgca gtcttccgag
     2401 ctgccggcgt gcagcaagcg gatcgtcgtc agcgtcgcgc tttacaccgg gctcgacaca
     2461 ccgggcgtcg acggggtgac cggggcgaac ggctgctgga cctacgagcg ggtcgccaag
     2521 cagaacgaag agtggaaaat ttgtaacttc gacgggacga tcagccacgc caatggaaac
     2581 aagtgggcct acgacgatac cagcaccaat cataacagca acaccgaaaa ggccgggatc
     2641 ctcgcgtgcc agcaggacgt gcccggtcgt ggttatgtgt acatgaccaa tcgcgggagc
     2701 ggctggccca aggtcgtcac cgagggcgtc gaggtgcact tcgccgagat ttactccggg
     2761 cagttcgccg tcgacgatca gttcaattta tggaagggcg agggggcgcc gggcgagccg
     2821 atggtcaact tcggcgagcc gtcgaccggc gccgcgacga tcgccaaggc gacccagcgg
     2881 tcgtgcgacg aggtcgagga cggcggctat ctcggcgtct acgtctatcc cgagtcgctg
     2941 cgtgcggcgc gaatgagcgc gatggtcgag gcgctcaaca cctgcaccgg cctgtgatgg
     3001 cagatccctc gctggcgggc gcgaagagct tgcgcgagcc gactcgcgcg cgacgcaggc
     3061 ggttcaggcg ggccccgggc gcgatctcgc gtgcggcagt acgggcgcgg cttcgtgggt
     3121 tacgcgatga aacgacgtgt gcgccgcgat tgcgcggcga tgacgagcgc gggacagtgt
     3181 gcatgcacgg gccctggcac cggcagccgc gctgtggcac catcctggcc cggttcgaac
     3241 gttcgcgtcc gcgatgaacg aatttctcag cgccatcctc agcttcccca cggtggtctt
     3301 cacggtgctg ctcggcgtga tcgtgctgta ctggctgatg gtcatcgtcg gtgcgatcgg
     3361 cgtcgatgtg ctgcacgtcg gcgaggccgc gggcggcaag atcgagggca tcgtcgacgg
     3421 cgcggtcgag ggcaagatcg agggcgtggt cgacggggcc gtgtccggcc acgccgaggg
     3481 cgccaccggc aagctcgagg cggtgggcgg cggcggcggc ttcctggcga tgctcggctt
     3541 cggcaaggtc ccggcgaccg tcgtgatcag ctcgctggtg ctgtggtcgt gggcgctgtg
     3601 cctgctgatc gcgcgcagcg gggccgagat ggggctcccg tcgtggtcgc tgcaggtgct
     3661 ggcgcccgtg ctcgcggtgt tcctcggcgg cgtgctgacg ggcgcgctgg tgcgcgggat
     3721 cgggcgcttt attgttcatc aggagccgct gcgccgcgcc gacctcgtcg gcaagctgtg
     3781 cacgatcacg accgggcgcg tcgacgccaa cttcgggcaa gcgctgatca gcgagcatgg
     3841 ggtcgacctc accgtcgacg tgcgctgcga tccgccgaac tcgctgcggc gtaacgcgca
     3901 ggcggtcctc gtcgcctacg acagcgagca ccacttcttc acggtcgagg catacgacca
     3961 accactggcg acgctcggtg agcgaccgcc ggcggctcat tgagcgcgca ggagagacag
     4021 cagatgttgg aatcgcaatt cgtcgtggcc gcaggcctgg aagagatcgg tttgccgctg
     4081 gcggcggtca tcgccggcgt catcttcctg gtcattgtcg gcaccttctt catcttctcg
     4141 cggttttatc gccaggtcga tcagggcaag gcgctgatcg tcaacaagaa gtccgggccg
     4201 gtcgtcgtgt tcaccggctc ggtcgtgctg ccgatcgtgc accgcgccga gatcatggac
     4261 atctcggtca agagcctcga catcgaccgc cgcggcaagg acggcctgat ctgcaaggac
     4321 aacatccgcg ccgacatcaa ggtcgcgttc ttcgtgcggg tcaacaagac cccggaggac
     4381 gtgctcaagg tggcgcagtc gatcggctgc gcccgcgcgt cggacctgga gaccctcgac
     4441 gagctgttct cggccaagtt ctccgaggcg ctcaagaccg tcggcaagca gctcgagttc
     4501 gaggagctgt acaccaagcg cggcgagttc aaagatcagc tgatcgaggt gatcggccgc
     4561 gacctcaacg ggtacatcct cgacgacgcc gcgatcgact acctcgagca gaccccgctc
     4621 aattcgctcg atccgcataa catcctcgac gcccagggta tccgcaagat caccgagatc
     4681 acggcgaagc agaacgtctt caccaatgaa ctgcgccaga aggagcgcat ggacgtcggg
     4741 tcgcagaacc tgaccgccga cgaggccgtg ttccgctacg agcagcagcg cgccgacgcg
     4801 gaggccaaga agcacaagga gatcgcgatc tcgatggccc gcgagcaaaa cgaggcgatg
     4861 cgggtcgcca gcgacgagaa caagaagacc tcgctggtcc agcagatcaa cgagggcgag
     4921 atcggcaagg cggagcagga caaggtccgg cagatcgcga tcgccgagaa ggcccgcgag
     4981 cgcgagatct cgatcgaggt cgagcgcaac gagaaggcgc gcgcgaccga ggcgatcggc
     5041 cgcgagcggg cggtcgagct gctgcgca
//
LOCUS       DASWNJ010001602         2107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_311968, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001602 DASWNJ010000000
VERSION     DASWNJ010001602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311968"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..>2107)
                     /locus_tag="VGB85_26600"
     CDS             complement(124..>2107)
                     /locus_tag="VGB85_26600"
                     /inference="COORDINATES: protein motif:HMM:NF028132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137695.1"
                     /translation="VGDSQVFGLGVEDDATFSARLAAHASRTVINGGVPTYGPAEYLA
                     VARELLVARKPRVVVVALNFVNDPFELGRPNKDRHAVWDGWAVRSETAPAEVFEFPGR
                     RWLFSRSHAVYALRRWLHERGTPPGGAELDDLVDLGTPSEGGLHDLVLASQSAHAEVA
                     STREQAQAELLGSKVRIYQIDRDLAGARGDIDDLVVSASNSEFDYSDSEVARGLPGDI
                     VSDRSSEASRGVQITAAMIRKAAREREKHLKALLRDEARAGKHQASDLLKTEATLLAE
                     RRVLRERIAAGIPPVPPPASLFRDYLAQFKALCDEHGAELVVVALPIDVQVDPGEWAK
                     YGVTDPPDMQESLVLLADLVSDARVLGLRALDATEALRAAQPGAFLDHDIHMTAKGHA
                     ALAEALAATLAAPVVPPAPDPAPGLPSGRTFAPTLDEWTFDTEVAVKGSSAAGCTTQI
                     RREWLRIHCARQKPRDAFSAVDVREGATPATMAMRTPDALGLVTPMTIGEPITARFTW
                     KSVTRDLEIRWPTGPDGKPRFSGAFVDVATDPAIHPPHPAVEALCACHHILTRETTCD
                     DPGAFEGSDPSGCKPSCGNFWGDADLDAACSAAHGSTCTAERIACVQNDPLAAPTCPE
                     GQVHAFASNRCFAICDAPRPCSTGACTPWNGGGVCL"
BASE COUNT          266 a          669 c          888 g          284 t
ORIGIN      
        1 ccgccgcgag gcgcaggtcg agcatgctgc cctgctcggc gagcgcgaag gcgtgcaggc
       61 gccgcgcgct gtggcccggc gagcgccaga ccagcgggct gagcaggcgg gcgagccagg
      121 cggtcacaga catacgccgc cgccgttcca gggggtgcag gcgccggtcg agcacgggcg
      181 cggcgcgtcg cagatcgcga agcagcggtt gctggcgaat gcatggacct ggccctcggg
      241 acaggtcggt gcggcgagcg ggtcgttctg cacgcaggcg atccgctcgg cggtgcacgt
      301 gctgccgtgg gccgcggagc aggcggcgtc gaggtcggcg tcgccccaga agttgccgca
      361 ggacggttta cagccggaag ggtcgctgcc ctcgaaggcc ccggggtcgt cgcaggtggt
      421 ctccctggtg aggatatggt ggcaggcgca cagggcctcg acggccgggt gcggggggtg
      481 gatcgcgggg tcggtggcga cgtcgacgaa ggcgccggag aaccggggct tgccgtccgg
      541 gccggtgggc cagcggatct cgaggtcgcg ggtgacggac ttccaggtga agcgggcggt
      601 gatcggctcg ccgatcgtca tcggggtgac gaggccgagg gcgtccgggg tgcgcatggc
      661 catggtcgcc ggggtcgcgc cctcgcgcac gtcgaccgcc gagaaggcgt cgcgcggctt
      721 ctggcgcgcg cagtggatcc gcagccactc gcgcctgatc tgggtggtac agccggcggc
      781 gctcgatccc ttgacggcga cctcggtgtc gaaggtccac tcgtcgagcg tcggggcgaa
      841 ggtgcggccg ctcgggagac ccggggcggg gtcgggcgcg ggcgggacga ccggggcggc
      901 gagcgtggcg gcgagcgcct cggcgagcgc ggcgtggccc ttcgcggtca tgtggatgtc
      961 gtggtcgagg aaggcgccgg gctgggcggc gcgcagggcc tcggtggcgt cgagcgcccg
     1021 caggccgagc acgcgggcgt cgctgacgag gtcggcgagc agcacgagcg actcctgcat
     1081 gtcgggcgga tcggtgacgc cgtacttggc ccactcgccg gggtcgacct ggacgtcgat
     1141 cggcagcgcg acgacgacga gctcggcgcc gtgctcgtcg cacagggcct tgaactgggc
     1201 gaggtagtcg cgaaagagcg aggctggcgg cgggaccggg gggatgccgg cggcgatccg
     1261 ctcgcgcagg acgcgacgct cggcgagcag ggtcgcctcg gtcttgagca ggtcactggc
     1321 ctggtgcttg ccggcgcggg cctcgtcgcg cagcagcgcc ttgaggtgct tctcgcgctc
     1381 gcgggcggcc ttgcggatca tcgcggcggt gatctgcacg ccgcggctgg cctcggagct
     1441 gcggtcgctg acgatgtcgc cggggaggcc gcgggcgacc tcgctgtcgg agtagtcgaa
     1501 ctcggagttg ctggcgctga cgacgaggtc gtcgatgtcg ccgcgcgcac cggcgaggtc
     1561 gcggtcgatc tgatagatcc gcaccttcga gcccagcagc tccgcctgcg cctgctcgcg
     1621 ggtgctggcg acctcggcgt gggcgctctg gctggcgagc acgaggtcgt gcaggccgcc
     1681 ctcggacggg gtgccgaggt cgacgaggtc atcgagctcg gcgccgccgg gcggggtgcc
     1741 gcgctcgtgt aaccaccgcc gcagggcgta cacggcgtgc gagcgcgaga ataaccaacg
     1801 ccggcccggg aactcgaaga cctcggccgg ggcggtctcg ctgcgcacgg cccagccgtc
     1861 ccacacggcg tggcggtcct tgttggggcg tccgagctcg aaggggtcgt tgacgaagtt
     1921 gagggcgacg acgacgaccc tgggcttgcg cgcgacgagc aactcgcgcg cgacggcgag
     1981 gtactcggcg gggccgtagg tcggcacgcc gccgttgatg acggtgcggc tggcgtgggc
     2041 ggcgaggcgg gcggagaagg tggcgtcgtc ctcgacgccg aggccgaaca cctgcgagtc
     2101 accgacg
//
LOCUS       DASWNJ010001603         2510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_99307, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001603 DASWNJ010000000
VERSION     DASWNJ010001603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99307"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VGB85_26605"
     CDS             complement(<1..547)
                     /locus_tag="VGB85_26605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137696.1"
                     /translation="MLLPALTMILALAGEGAPPPVLALVTAPWATHSELVQLGDSGAR
                     GRFEHTAGAAVRGAVVPGGGVVAVADTAPRRDLSFASSLFVLEDGHAARRLCDEVVHA
                     SRPFVGPDGRVFVVRGAAGQESDGSRLRDDFLRVDEIDLQTGVARTQHRFTGQLLHLA
                     GWYDGALLVYRVAGDAADIVAI"
     gene            complement(674..2371)
                     /locus_tag="VGB85_26610"
     CDS             complement(674..2371)
                     /locus_tag="VGB85_26610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137697.1"
                     /translation="MRARWGCLLCIVLACGGSGSVDPADATAEDLHGRWVRLLPEGDQ
                     RVFSFQERGSFHPQLVDATDVFAIYDVTETAQYPNMFGTYRVEAGQIVQTAVWSRDET
                     LGGGEFRNDLLSLQTGAQLVIEGGDGMPSTYDHVDTCEVPRSIDGWRHLSLAPSLWNS
                     YGGVGRIFTTVAVDPAGAIVATAFNAVARYDGECIPEVTSEDSLGDSSVHRAPDGVHV
                     ITGRHQGLDAVPATYTRLLPGPFRLDSRPLPIDGREVLDATDLGGKIGILLRPAGGGY
                     ALVRDDGASFIQAPLTGIGPQFIAAELLDGGNGRAVIVGTDYPAGELRLALAEETDAG
                     FVTSEVPIPTFASYVAAAAEGDDLYIVYAAAIDPGAARPQLLHLATRRAGTWTTTPLL
                     IGALPQVVARDGVAHIVSLVVDPLEITAHYTRVDAGGVASWRAGGAGTGGQPVPVPAI
                     DLTSDDTVILGVQGSLHLRGRDERDDRMVPVSVTIEGSGRVLSSDGTIDCTQSCTVMA
                     EVGRRLLVTATATSGGVPFVACPVAVGAFDPAVPEVCNLDVRIDDGLHPLVVTATFP"
BASE COUNT          381 a          919 c          891 g          319 t
ORIGIN      
        1 cgatcgccac gatgtcggcc gcgtcacccg cgacgcgata cacgagcagc gcgccgtcgt
       61 accagccggc gaggtgcagc agctgcccgg tgaagcgatg ctgcgtgcgc gcgacgccgg
      121 tctgcaggtc gatctcgtcg acgcgcagga agtcgtcgcg cagccgcgag ccgtcgctct
      181 cctgccccgc cgcgccgcgg accacgaaca cgcgcccgtc cgggccgacg aacggccgcg
      241 aggcgtgcac gacctcgtcg cacagccgcc gcgccgcgtg cccgtcctcg agcacgaaca
      301 ggctcgacgc gaacgacagg tccctgcgcg gcgccgtgtc ggcgacggcg acgacgccac
      361 cgcccgggac cacggcccca cgcaccgcgg ccccggccgt gtgctcgaag cgcccgcgcg
      421 cgccgctgtc cccgagctgc acgagctcgc tgtgggtcgc ccacggagcg gtcaccagcg
      481 cgagcaccgg aggtggcgcg ccctcgccag cgagcgcgag gatcatggtc agggccggaa
      541 gcaacatgcg agcacgatat cgcaaccgac gcgcgagttc acgcccgatc gcacgacctc
      601 gccgattttc gagcgggccg gcgaagcacg cacgcgcccc gcggctagca ggccacgggc
      661 ccgcggacga cgctcacggg aaagtcgccg tgacgaccag ggggtggagg ccgtcgtcga
      721 tccttacgtc gaggttgcag acctcgggca cggccgggtc gaaggcgccg accgccaccg
      781 ggcaggccac gaagggcacg ccgccggacg tcgccgtggc ggtgacgagc aggcgccggc
      841 cgacctcggc catcaccgtg cacgactgcg tgcagtcgat cgtgccgtcg ctgctgagga
      901 cccggccgct gccctcgatc gtgaccgaga ccgggaccat gcgatcgtcg cgttcgtcgc
      961 gcccgcgcag gtgcaacgag ccctgcacgc cgaggatgac ggtatcgtcg ctcgtcagat
     1021 cgatcgccgg cacgggcacg ggctggccgc cggtgcctgc accgccggcc cgccacgagg
     1081 ccacgccgcc ggcgtcgacg cgcgtgtagt gagcggtgat ctcgagcggg tcgaccacca
     1141 ggctgacgat gtgcgcgacg ccgtcgcgcg cgaccacctg cgggagcgcg ccgattaaca
     1201 gcggcgtggt cgtccacgtg ccggcgcggc gcgtggcgag gtgcagcagc tgcggccgcg
     1261 cggcgcccgg gtcgatcgcc gccgcgtaga cgatataaag atcgtccccc tcggcggccg
     1321 cggcgacgta gctggcgaag gtcgggatcg gcacctcgct ggtgacgaag cccgcgtcgg
     1381 tctcctccgc gagcgcgagg cgcagctcgc cggcgggata gtcggtgccg acgatcacgg
     1441 cgcggccgtt gccgccgtcg agcagctccg cggcgatgaa ttgcgggccg atgccggtca
     1501 gcggcgcctg gatgaagctc gcgccgtcgt cgcgcacgag cgcatagccg ccgccggcag
     1561 gccgcagcag gatcccgatc ttgccgccga ggtcggtcgc gtcgaggacc tcgcgcccgt
     1621 cgatcggcaa gggccgcgaa tcgaggcgaa acgggcccgg cagcagcctg gtgtacgtcg
     1681 ccggcacggc gtcgaggccc tgatgccggc cggtgatcac gtggactccg tcgggggcgc
     1741 gatgcactga agagtcgccg agcgagtcct cgctcgtgac ttccgggatg cactcgccgt
     1801 catagcgcgc gacggcgttg aacgcggtcg ccacgatcgc acccgcgggg tcgaccgcga
     1861 ccgtcgtgaa gatccggccg acgccgccgt agctgttcca cagcgacggc gccagcgaga
     1921 ggtgccgcca gccgtcgatg ctgcgcggca cctcgcaggt gtcgacgtga tcgtaggtgc
     1981 tcggcatgcc gtcgccgccc tcgatcacca gctgcgcgcc ggtctgcagc gacagcaggt
     2041 cattgcgaaa ctcgccgccg cccagcgtct cgtcgcggct ccacacggcc gtctgcacga
     2101 tctggccggc ctcgacgcga taggtcccga acatgttcgg atactgcgcg gtctcggtga
     2161 catcgtagat ggcgaacacg tcggtcgcgt cgacgagctg cgggtggaag ctgccccgct
     2221 cctgaaacga gaacacccgc tgatcgccct cgggcaacag acgcacccac cggccgtgca
     2281 ggtcctcggc cgtggcatcg gccggatcga cggagccgga gccaccacag gcgaggacga
     2341 tacacagcag gcaaccccag cgagcgcgca tgaccgacat ggtaacgcga gcgtcggtgt
     2401 ttatgaaatt gccacgtacg acgcgactgg ccggtgggcc gcgcacgctt ctgttatcct
     2461 ggctcgacca tgcggccgcg gatcctcgcc ctcctcgccc tcctcctgtg
//
LOCUS       DASWNJ010001604         4878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_168341, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001604 DASWNJ010000000
VERSION     DASWNJ010001604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168341"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VGB85_26615"
     CDS             <1..567
                     /locus_tag="VGB85_26615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137698.1"
                     /translation="TGDQTVLRCALAVPPGVRVAIMGTQNIKGTGLDFVYRWIALDTA
                     EQNLALLNSGDPAVRRAALQRLEHPDDPGLIDAGLVAARLRTRAASPEEAELQARAQA
                     RAEAMHQARRAALGARARKRGKAEAFFAWLELWVDFIDGARRHARARVVMNDLIDQRI
                     SHARAAQAMRELDARAKGGWLVKAMRHK"
     gene            620..1153
                     /locus_tag="VGB85_26620"
     CDS             620..1153
                     /locus_tag="VGB85_26620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137699.1"
                     /translation="MTLRSLLGTPISVALVTCSLTSGCARTFYTSEAALHEAEQVVLT
                     TPAQDIAIPASSRPEPAVDPTWLRYSKLTVMMPTTRKPGSPIAVRTPNHRRKWIAGII
                     LASIGGSMIAGGLVQVVYGAKTDDLGIIAGLAGLPIVMGTSMVIPGALLIKRNRKPFD
                     ILSPGLPGMHYVPAAAK"
     gene            complement(1233..1484)
                     /locus_tag="VGB85_26625"
     CDS             complement(1233..1484)
                     /locus_tag="VGB85_26625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137700.1"
                     /translation="MDQRLGREAGQAIDDCAAQAGAMTEELVKIDVEIAADGRVTAAR
                     AYGTTLGKNYHECAARAVRSVRFDAVGDAQKYTYQHAVR"
     gene            complement(1847..2695)
                     /locus_tag="VGB85_26630"
     CDS             complement(1847..2695)
                     /locus_tag="VGB85_26630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0137701.1"
                     /translation="MTNKLEATNDARDRAVPLQFGAAQTDLERYLLALVNHAPGIIFL
                     KDTEYRYLYVNQAYLDNGGWTREKVIGHRDADFMLAEAAATIHTQEVQVMTTRRSLEL
                     TETLPFHDGTRHYTSFKFAVLDERGELMGIGGFITDVTARKREEAALLAEQQRVIDDQ
                     RDTLRELSTPLLPIAEGVLAVPLVGALGPRRSQELLSSLLRGIGEQRVRIVILDVTGI
                     REIDVEVAASLVTAARAARLLGAEVVLTGISPAVAQTLVAIGTDLSGITTLATLASGI
                     SFALNR"
     gene            complement(2942..3742)
                     /locus_tag="VGB85_26635"
     CDS             complement(2942..3742)
                     /locus_tag="VGB85_26635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137702.1"
                     /translation="MIVLDHALPSLGGRTVRELIVVAGGLAIVAAECLRPGPVDVGNL
                     IVYVVATLLFALRCFAARAAGVGACIGAIVQQWPNLRLGEVTPETLAILPLAGIAVLA
                     SSDLVERYERAPSRIAWLPNPWAGFTAAETRSLRWATYAAGALAGLLDHTTQLMAWAP
                     LYGLAAPWWPRIAMVSLVLAVAVLCLGRAVGALLVWVTAIVVAIHSAPLAWQAEALLG
                     PLVAGPARLPLVYQLAAHYLLPVVLLASAAAIVTTPAVARLLRHTVRG"
     gene            3875..4291
                     /locus_tag="VGB85_26640"
     CDS             3875..4291
                     /locus_tag="VGB85_26640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137703.1"
                     /translation="MLRSILAVLAGLVLGMFVITAVESIIPMVYPMPRAVDPRDIEAM
                     RTTLAGMPVGVFVILMLGWILGALAGGLLAARLASTWFHRAPLRHALAVGVVQTIGAV
                     TNFAMLPHPTWVLIAGLVVFIPMALLGGRLGQRPAV"
     gene            4414..>4878
                     /locus_tag="VGB85_26645"
     CDS             4414..>4878
                     /locus_tag="VGB85_26645"
                     /inference="COORDINATES: protein motif:HMM:NF013341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HEY0137704.1"
                     /translation="MRTPEALETLVEYGIIEEVLRPLMSGKEAQIYLVLSGGERRVAK
                     VYKEAQDRSFKQRAEYTEGRKSRNSRDARAIAKHSRHGKAQDEAAWRSAEVNAIYRLN
                     AAGVRVPTPYHFVDGVLVMELVTDEYGDPAPRLGDLQFDPSGARQVYDYLVAQ"
BASE COUNT          707 a         1765 c         1708 g          698 t
ORIGIN      
        1 accggcgacc agaccgtgct gcgctgcgcc ctcgcggtcc cgccgggcgt gcgcgtggcg
       61 atcatgggca cgcagaacat caagggcacc ggcctcgact tcgtgtaccg ctggatcgcc
      121 ctcgacaccg ccgagcagaa cctcgccctg ctcaacagcg gcgacccggc cgtgcgccgc
      181 gcggcactgc agcgcctcga gcaccccgac gaccccggcc tgatcgacgc cggcctcgtc
      241 gccgcccgcc tgcgcacgcg cgccgcgagc cccgaggagg ccgagctgca agcccgcgcc
      301 caggcccgcg ccgaggcgat gcaccaggcc cgccgcgccg ccctcggcgc gcgcgcccgc
      361 aagcgcggca aggccgaggc cttcttcgcc tggctcgagc tgtgggtcga cttcatcgac
      421 ggcgcgcgcc gccacgcgcg ggccagggtc gtcatgaacg acctgatcga ccagcgcatc
      481 agccacgccc gcgccgccca ggcgatgcgc gagctcgacg cccgcgccaa gggcggctgg
      541 ctggtcaagg cgatgcgcca caagtgaccc gccagcggcg gcctcgacga cccgcggccg
      601 cgtcggatac cctcgaaccg tgacgctcag gagcctgctc ggcaccccga tctcggtcgc
      661 gctcgtgact tgctcgctga cctccgggtg cgcgcgcacc ttctatacgt cggaggcggc
      721 gctgcacgag gcggagcagg tcgtgctgac gacgcccgcg caggacatcg cgatcccggc
      781 gagcagccgc ccggaacccg ccgtggaccc gacctggctg cgctactcga agctgaccgt
      841 catgatgccg acgacgcgca agcccggctc gccgatcgcc gtccggacgc cgaatcaccg
      901 gcgaaagtgg atcgccggga tcatcctcgc cagcatcggc ggctcgatga tcgccggcgg
      961 cctcgtgcag gtcgtctacg gcgcgaagac cgacgacctc ggcatcatcg ccggcctcgc
     1021 cgggctgccg atcgtcatgg gcaccagcat ggtcatcccc ggcgcgctcc tgatcaagcg
     1081 caaccggaag cccttcgaca tcctgtcgcc cgggctgccg ggcatgcact acgtgcccgc
     1141 cgcggcgaaa tagccgcggc tgcgtcgatc ggcgcgtgct tgcgtccctg gcacgcatga
     1201 cggagcgcgc gcgcatcggt gagacgcgag aatcagcgca ctgcgtgttg atacgtatat
     1261 ttctgtgcgt cgccgacagc atcgaaccga acgctgcgga ccgctctcgc ggcgcattca
     1321 tggtagttct ttcccagcgt cgtaccgtat gcgcgcgccg cggtgacccg gccatcggcc
     1381 gcgatctcga cgtcgatctt cacgagctcc tcggtcatcg cgcccgcctg cgcggcgcaa
     1441 tcgtcgatcg cctggccggc ctcgcgaccg aggcgctgat ccatcgaccg atgattgcgc
     1501 tggcttgggg gcttctggac ggtcgggaca tcggggtctt accgcccgcg gagccttctt
     1561 cgccgccggc tcgaccgtcg cgggcggggg cccgaggtcg acctcgtcgg ccaccggcat
     1621 gtccggcgcc cggtcgacga cgatcgcccg caccaccggg gcgtccggcg acgggctcgc
     1681 caccggcact ggctccggca cgggatctgg atctgcgccg ttcaccggct gcctcgccgg
     1741 ctcggtgggc catcgccaca tcgtcaccag gccgatcgtc tcgtccgtca gcgcgcaggt
     1801 gtggttgccc ccgctggctt ctcgcggatc tcgcgacccc gcggtatcag cggttcaacg
     1861 cgaacgagat cccgctggcg agcgtcgcga gtgtggtgat accactgagg tccgtaccga
     1921 tcgccacgag cgtctgcgcg acggcggggc tgatgccggt gagcacgacc tcggcgccga
     1981 gcagccgcgc ggcccgtgcg gcggtcacca gcgaggcggc gacctcgacg tcgatctcgc
     2041 ggatgccggt gacatcgagg atgacgatgc gcacccgctg ctcgccgatc ccacgcagca
     2101 aactgctcag cagctcttgc gagcgccggg gccccagcgc gccgaccagc ggcaccgcga
     2161 gcacgccctc ggcgatcggc agcagggggg tcgacagctc gcgcagcgtg tcgcgctggt
     2221 cgtcgatcac gcgctgttgc tcggcgagca gcgcggcctc ctcgcgcttg cgggcggtga
     2281 catcggtgat aaatccgccg atgcccatca gctcgccgcg ctcgtcgagg accgcgaact
     2341 tgaacgaggt gtagtgccgc gtcccgtcgt ggaagggcaa cgtctccgtg agctcgaggg
     2401 agcgtcgcgt cgtcatcacc tgcacttcct gggtgtggat cgtcgccgcc gcctccgcga
     2461 gcatgaagtc ggcgtcgcgg tgcccgatca ccttctcgcg ggtccagccg ccgttgtcga
     2521 ggtacgcctg attgacgtac aggtagcgat attcggtgtc cttgaggaaa atgatgcccg
     2581 gcgcgtgatt gacgagcgcc agcaggtagc gctcgaggtc cgtctgcgcc gcgccgaatt
     2641 gcagtgggac agcgcggtcc ctggcgtcgt tcgtcgcttc gagcttgttc gtcaccgcag
     2701 gttccccgac agctttcagc cttaccgcag gtctgcgcgg cccggcaagg cgcgagttcg
     2761 tcggcacgcg gcgcagtggg ccgcacaccc gcagacggtc cgtgtctcgc cttgtaggcg
     2821 atgtctgaca ccgccgcgat cgtccgatcc ggcgttcgcc ttcgcggtgc ccggcgaacg
     2881 acggtcagcg atcacataat gtgggtactt cgcggtcgtg cgaacaccgg gtcgcgaact
     2941 gtcagccgcg cacggtgtga cgcagcaggc gagccaccgc cggggtggtg acgatcgccg
     3001 cggcgctggc gaggagaacc acgggtagga gatagtgcgc cgcgagctgg tagacgaggg
     3061 gcaggcgcgc gggtccggcg acgagcgggc cgagcagggc ctcggcttgc caggccagcg
     3121 gcgcgctgtg gatcgcgacg acgatcgccg tgacccacac cagcagcgcc ccgacggcgc
     3181 ggccgaggca cagcaccgcg acggcgagca ccagcgagac catggcgatc cgcggccacc
     3241 acggggccgc gaggccatac agcggggccc aggccatcag ctgcgtcgtg tggtcgagca
     3301 ggcccgcgag ggcccccgcc gcgtaggtgg cccagcgcag cgagcgggtc tcggccgccg
     3361 tgaagcccgc ccacgggttc ggcagccacg cgatccgcga cggcgctcgc tcgtagcgct
     3421 cgacgaggtc gctcgacgcg agcaccgcga tgccggccag cggcaagatc gcgagcgtct
     3481 cgggcgtgac ctcgccgagc cgcaagttcg gccactgttg aacgatcgcg ccgatgcacg
     3541 cgccgacgcc cgcggcccgg gcggcgaagc agcgcagcgc gaacagcagg gtcgcgacca
     3601 cgtagacgat caggttgccg acgtcgaccg ggccaggccg caggcactcg gcggcgacga
     3661 tcgccaggcc gccggcgacg acgatcagct cgcggacggt gcggccgccg agcgagggca
     3721 gcgcgtggtc gaggacgatc atgccgcgat gatgcctgaa accgccacaa ccgcggcgcc
     3781 gtatgtcccg agagacattg gcggacgccg accgggtccg cgagccgtct tgacttcatg
     3841 cgcgatccgc gacatgactg cattcggagg catcatgctg cgtagtattc tcgccgtgct
     3901 ggccggcctc gtgctcggca tgttcgtgat cacggccgtc gagtcgatca tcccgatggt
     3961 ctatcccatg ccgcgggcgg tcgacccgag ggacatcgag gccatgcgga cgacgctggc
     4021 gggcatgccg gtcggggtgt tcgtgatcct gatgctcggc tggatcctcg gggcgctcgc
     4081 gggcggcctg ctggccgcgc gcctggcatc gacctggttc caccgggctc cgctgcgcca
     4141 cgcgctcgcg gtcggcgtcg tacagacgat cggcgccgtc accaatttcg ccatgctgcc
     4201 gcacccgacc tgggtcctga tcgccgggct cgtcgtgttc atcccgatgg cgctgctcgg
     4261 cggtcgcctc ggccagcgac cggcggtgtg agcctcgctc gcggggcatg tcgcggagaa
     4321 cgcgctcggc tcgccggccg cgtattccag gagcttttgc gaggcctcgc acggccgcgc
     4381 gcggcggttg tgtgctaatg aggccgggcc cgcatgcgca cgcccgaggc cctcgagacc
     4441 ctcgtcgagt acggcatcat cgaggaggtg ctgcgtccgc tgatgagcgg caaggaggcg
     4501 cagatctacc tcgtgctgtc cggcggcgag cggcgcgtgg ccaaggtcta caaggaggcc
     4561 caggaccggt cgttcaagca gcgcgccgag tacaccgagg gccgcaagag ccgcaacagc
     4621 cgcgacgcgc gggcgatcgc caagcactcg cggcacggca aggcccagga cgaggccgcg
     4681 tggcgctcgg cggaggtcaa cgcgatctac cgcctcaacg ccgccggcgt gcgcgtgccg
     4741 accccgtacc acttcgtcga cggcgtgctg gtgatggagc tcgtcaccga cgagtacggc
     4801 gacccggccc cgcgcttggg cgacctccag ttcgacccga gcggcgcgcg ccaggtctac
     4861 gactatctcg tcgcccag
//
LOCUS       DASWNJ010001605         6143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_132951, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001605 DASWNJ010000000
VERSION     DASWNJ010001605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6143
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132951"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VGB85_26650"
     CDS             <1..769
                     /locus_tag="VGB85_26650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137705.1"
                     /translation="LYDVAFGGDRYVAVGQAGKILESTDGVTWRSASSPSSRDLRAVV
                     HHQNRFVAVGGDYSVGAETLESTDGITWTRPEYPAPLHLLTDLASDGVSLVAIGHYQS
                     DAQTFGAFTWVDGLGWDQTVDGGLSNFGFNAVAAGSPAFAMIGLGSAVTSLDAVNWSF
                     APVFQASAMRGLTYAGTGWIAVGDGGQVLLSPDAAQWSPYASGVAVNLAAVTSDGGLH
                     IAVGGAGTILASNDGITWIAQISNAAEALHAVTHPRS"
     gene            complement(773..1891)
                     /locus_tag="VGB85_26655"
     CDS             complement(773..1891)
                     /locus_tag="VGB85_26655"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0137706.1"
                     /translation="MAQPTTVITGATGFLGRELVLHLLASEPGDHLTLLVRGRDEVDA
                     QARGMAVLTAHLQGAALADATRRVEIVRADLERERLGLDARAHDELAARTKAVIHGAA
                     SVSFSLPIEQARAINVEGTRRMLDLANMARAHLDYVGTAYVAGERSGLVLESELAVGQ
                     GFRNTYERSKCEAERLVQSRMGEQSLTVYRPSIIVGDSRTGRTASFKVLYWPLKIFAR
                     GFRVVPGRAQTPMDVVPSDFVVQALAHLRAQPGRAGQVYHLCAGPERSATLGELAGLA
                     SRFFGVRRPIFVQPQLFVKARPLVDRFTWGRLRRTLIAGRVYTPYLSLDMAFDTRRLR
                     AGLAGSGIEVPRVEDYFANLFRYAIETDWGKKLGGGGE"
     gene            2011..2511
                     /locus_tag="VGB85_26660"
     CDS             2011..2511
                     /locus_tag="VGB85_26660"
                     /inference="COORDINATES: protein motif:HMM:NF017090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="emopamil-binding family protein"
                     /protein_id="HEY0137707.1"
                     /translation="MSRSDRVTLGLLTFFLCIAFSLELYWLIFRDELVTRAPHELFAR
                     LFQIYGDADRAYYDRVSALAIGLEGINVYFTQLLNVWLIYAIVARKHYRHVLQLIVSS
                     YLAYSVILYFWCAHVVGYPDMRYKSAYTYFLFYVPNFPWLFGYAWMAWISARALNAHM
                     RGAGPA"
     gene            2642..4180
                     /locus_tag="VGB85_26665"
     CDS             2642..4180
                     /locus_tag="VGB85_26665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0137708.1"
                     /translation="MDPLLDELTRLREATAADPVLREALAQVEAALHQARKTADGATR
                     ARGAFLANLSTMGHELRTPLNAILGFAQLLRQDRGLPPEQHTHVEIIHRSGEQLLAMI
                     NDLLALSRQDVDDGLAGASFDLLQLVDDLEDMLYLRAERRGLRLRVRRDPDLPRLIRV
                     DGPRLRQLFLLLLGNAIKFARPGELGLALACTADGGDTQRGSLRVVLAIPGLGKADLE
                     AFVRNTNAHRSGPIPQQAGSRDGPPLGQELARALGGELDIAPGELRLLLPVGLIAASE
                     AATAPPDPTIVGLAPGQPTYRILVAEDRWQSRQLLVQMLSRVGFDVREASDGAEAMEL
                     WHSWRPQLIWMDMRMPGITGLEATLRIKRSPGGKETVVIALTASAFEEDPATARAAGC
                     NDFVRKPIRMAEVFAKLREHLGVTYLYESAETPAPPPGPAPSLDPAVLARLPATWIAA
                     MQAACVQADLDEVRGLIARIRGDAREAADTLDELARGFSFDRILEILTSIEINQAARA
                     GGTP"
     gene            4177..5262
                     /locus_tag="VGB85_26670"
     CDS             4177..5262
                     /locus_tag="VGB85_26670"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY0137709.1"
                     /translation="MNQSPGSRGNVLVVDDTLVNLRILTEMLAAQGYKVRPVTSGPQA
                     LQAAAAAPPDLVLLDINMPGMTGYEVCQQLKNQPVTRDVPVIFMSARDEVLDKIRAFE
                     VGGVDYVTKPFHLEELLARVATHVSLRALQRQLEESRSSAEATAKALEEEKHRTDELL
                     HSMLPRSVAEDLRNGKQVAPDSFPNVTVLFCDIVDFTATASSHSPLQIFELLGQLFTR
                     FDALIERHPVYKVETVGDCYMVAAGVPEANPQHANDVVATALDMLDCVKDIVLPNGGS
                     LQLRIGVNSGPVVAGVVGTKMPRFCLFGDTVNIASRMETTSTPMCVQISETTRLLLDP
                     ARWKIEERGEIQLKGRGPLQTYFVSRA"
BASE COUNT          898 a         2442 c         1927 g          876 t
ORIGIN      
        1 gctgtacgac gtcgccttcg gcggcgaccg ctacgtcgcc gtcggccagg ccggcaagat
       61 cctcgagtcg accgacggcg tcacgtggcg atccgcctcg tcgccgtcga gccgcgacct
      121 gcgggccgtc gtgcaccacc agaaccgctt cgtcgccgtc ggcggcgact acagcgtcgg
      181 cgccgagacc ctcgagtcga ccgacggcat cacctggacg cgccccgagt acccggcccc
      241 gctgcacctg ctgaccgacc tcgcctccga cggtgtcagc ctggtcgcga tcggccacta
      301 tcagagcgac gcgcagacct ttggcgcctt cacctgggtc gatggcctcg gctgggacca
      361 gaccgtcgat ggtggcctct ccaacttcgg cttcaacgcc gtcgccgccg gctcgcccgc
      421 gttcgcgatg atcggcctcg gcagcgccgt cacctcgctc gacgccgtca actggagctt
      481 cgcgccggtg tttcaggcct ccgcgatgcg cggcctgacc tacgccggca ccggctggat
      541 cgccgtcggc gacggcggac aggtgctgct gtcccccgac gccgcgcagt ggagcccgta
      601 cgccagcggc gtcgccgtca acctcgccgc ggtcacctcg gacggcggcc tgcacatcgc
      661 cgtcggcggc gccggcacga tcctcgcctc gaacgacggc atcacctgga tcgcccagat
      721 ctcgaacgcc gccgaggcgc tgcacgccgt cacgcacccg cgttcgtgac cctcactcgc
      781 ccccgccccc gagctttttc ccccaatccg tctcgatcgc gtagcgaaac aggttggcga
      841 aataatcctc gacccgcggc acctcgatgc cgctgcccgc gaggccggcg cgcagtcgcc
      901 gagtgtcgaa ggccatgtcg agcgacagat acggcgtgta cacgcggccg gcgatcaggg
      961 tccggcgcag ccgcccccag gtgaagcggt cgacgagcgg tcgcgccttc acgaacagct
     1021 gcggctgcac gaagatcggc cgccgcaccc cgaagaagcg cgacgccagc ccggcgagct
     1081 cgcccagcgt cgccgagcgc tccggcccgg cgcacaggtg atagacctgc cccgcgcgcc
     1141 ccggctgggc ccgcaggtgc gcgagcgcct ggacgacgaa gtccgacggc acgacatcca
     1201 tcggcgtctg cgcccgcccc ggcacgaccc ggaacccgcg cgcgaagatc ttgagcggcc
     1261 agtacagcac cttgaagctc gccgtccgac ccgtgcgcga gtcgccgacg atgatgctcg
     1321 ggcgatacac cgtcagcgac tgctcgccca tgcgcgactg cacgagccgc tccgcctcgc
     1381 acttcgatcg ctcgtaggtg ttgcgaaagc cctgcccgac tgcgagctcc gactcgagca
     1441 cgagcccgct gcgctcgccg gcgacgtacg cggtgccgac gtagtcgagg tgcgccctgg
     1501 ccatgttcgc caggtcgagc atgcgccgcg tgccctcgac gttgatcgcc cgtgcctgct
     1561 cgatcggcag gctgaagctg accgaggccg cgccgtggat caccgccttc gtgcgcgccg
     1621 cgagctcgtc gtgggcccgc gcgtcgagcc cgagtcgctc gcgctcgagg tccgcgcgca
     1681 cgatctccac ccgacgcgtc gcgtccgcca gcgcggcgcc ctgcaggtgc gccgtcagga
     1741 ccgccatgcc ccgcgcctgc gcgtcgacct cgtcgcgccc gcgcaccagc aaggtcaggt
     1801 gatcaccggg ctcgctcgcc agcaggtgca gcaccagctc gcgtcccaaa aacccggtcg
     1861 cgcccgtgat caccgtcgtc ggctgagcca tcgcggccgg gacgatgccg cccgcctgcg
     1921 tcgcacgcaa ggccagacgt cgggtgacgc acgccacccc gcgcgaccgc cctggaacga
     1981 cgtacacatc ggtcgataaa ctccccgccg atgtctcgca gcgatcgcgt caccctcgga
     2041 ctgctcacct ttttcctctg catcgccttc tcgctcgagc tctactggct catcttccgc
     2101 gacgagctgg tgacccgcgc cccccacgag ctgttcgccc gcctgttcca gatctacggc
     2161 gacgccgacc gggcctacta cgaccgggtc tcggccctgg cgatcggcct cgagggcatc
     2221 aacgtctact tcacgcagct gctcaacgtg tggctgatct acgcgatcgt cgcccgcaag
     2281 cattaccgcc acgtcctcca gctgatcgtc tccagctacc tcgcctactc ggtgatcctc
     2341 tacttctggt gcgcccacgt cgtcggctac ccggacatgc gctacaagag cgcctacacc
     2401 tacttcctgt tctacgtccc caacttcccg tggctgttcg gctacgcctg gatggcctgg
     2461 atctccgccc gcgcgctcaa cgcccatatg cgcggtgccg ggcccgcctg agcccccgct
     2521 cgcacccgcg cgaaccccca cgcgtcaccc cgcgtcggca ggccccggag ggccgacgct
     2581 tcgggtacca tcgaccatcc tcgaggcccc gttgaccagc atcagcccca gccccgcggc
     2641 cgtggacccg ctccttgacg aactcacccg cctgcgtgaa gcgaccgcgg ccgacccggt
     2701 gctgcgcgag gcgctggcgc aggtcgaggc cgcgctgcat caggcccgca agaccgccga
     2761 cggcgcgacg cgggcgcgtg gggccttcct cgccaacctg tcgaccatgg gccacgagct
     2821 gcgcacgccg ctcaacgcga tcctcggctt cgcccagctg ctgcgccagg atcgcggcct
     2881 cccccccgaa caacacaccc acgtcgagat catccaccgc agcggcgaac agctgctggc
     2941 gatgatcaac gacctgctcg cgctgtcccg ccaggacgtc gacgacggcc tcgccggcgc
     3001 cagcttcgac ctgctgcagc tcgtcgatga cctcgaggac atgttgtacc tgcgcgccga
     3061 acgtcgcggc ctgcgactgc gcgtccgtcg cgaccccgac ctcccgcgcc tgatccgcgt
     3121 cgacggcccc cgactgcgcc agctgttctt gctgctgctc ggcaacgcga tcaagttcgc
     3181 caggcccggc gagctcggcc tcgccctcgc ctgcaccgcc gacggcggcg acacccagcg
     3241 cggctcgctg cgcgtcgtcc tcgcgatccc gggcctcggc aaggccgacc tcgaggcctt
     3301 cgtccgcaac accaacgccc accgctccgg accgatccca caacaggccg gctcgcgcga
     3361 cggcccaccg ctcggccagg agctcgcccg tgccctcggc ggcgagctcg acatcgcccc
     3421 cggcgagctg cgcctgctgc tgcccgtcgg cctgatcgcc gccagcgagg ccgcgaccgc
     3481 cccccccgat ccgacgatcg tcggcctcgc acccggccag ccgacctacc ggatcctcgt
     3541 cgccgaggac cgctggcaga gccgccagct gctcgtacaa atgctgtcgc gcgtcggctt
     3601 cgacgtccgc gaggccagcg acggcgccga ggcgatggag ctgtggcaca gctggcgccc
     3661 gcagctgatc tggatggaca tgcgcatgcc cggcatcacc ggcctcgagg cgacgctccg
     3721 gatcaagcgc agccccggcg gcaaggagac cgtcgtcatc gccctgaccg cgagcgcctt
     3781 cgaggaggac ccggccaccg cgcgggccgc cggctgcaac gacttcgtgc gcaagccgat
     3841 ccgcatggcc gaggtgttcg ccaagctgcg tgagcacctc ggcgtcacgt acctctacga
     3901 gtcggccgag acccccgcgc cgccgccggg ccccgctccc tcgctcgacc ccgccgtcct
     3961 cgcccgcctg cccgccacgt ggatcgccgc gatgcaggcc gcctgcgtgc aagccgacct
     4021 cgacgaggtc cgcggcctga tcgcccgcat ccgcggcgac gcccgcgagg ccgccgacac
     4081 cctcgacgag ctcgcccgcg gcttcagctt cgaccgcatc ctcgagatcc tgacgagcat
     4141 cgagatcaac caagcggccc gcgccggagg caccccatga accagtcccc cggctcccgc
     4201 ggcaacgtgc tcgtcgtcga cgacaccctc gtcaacctcc ggatcctgac cgagatgctc
     4261 gccgcgcagg gctacaaggt ccgcccggtc accagcggcc cacaagcgct gcaggccgcg
     4321 gccgcggccc cgccggacct cgtcctgctc gacatcaaca tgccgggcat gaccggctac
     4381 gaggtctgcc agcagctcaa gaaccagccc gtcacccgcg acgtcccggt catcttcatg
     4441 agcgcccgcg acgaggtcct cgacaagatc cgcgcgttcg aggtcggcgg cgtcgactac
     4501 gtcaccaagc ccttccacct cgaggagctg ctcgcccgcg tcgccacgca cgtgtccctg
     4561 cgcgccctgc aacgccagct cgaggagtcg cgcagcagcg ccgaggcgac cgccaaggcg
     4621 ctcgaggagg agaagcaccg caccgacgag ctgctccaca gcatgctccc ccgcagcgtc
     4681 gccgaggacc tgcgcaacgg caagcaggtc gcccccgaca gcttcccgaa cgtcaccgtg
     4741 ctgttctgcg acatcgtcga cttcaccgcg acggcctcgt cccactcccc cctgcagatc
     4801 ttcgagctgc tcggccagct gttcacccgc ttcgacgcgc tgatcgagcg ccaccccgtc
     4861 tacaaggtcg agaccgtcgg cgactgctac atggtcgccg ccggcgtacc cgaggccaac
     4921 ccgcagcacg ccaacgacgt cgtcgccacc gccctcgaca tgctcgactg cgtcaaggac
     4981 atcgtcctcc ccaacggcgg ctcgctgcag ctgcgcatcg gcgtcaactc gggccccgtc
     5041 gtcgccggcg tcgtcggcac caagatgccc cgcttttgcc tgttcggcga caccgtcaac
     5101 atcgccagcc gcatggaaac cacgagcacg cccatgtgcg tgcagatcag cgagaccacc
     5161 cgcctgctgc tcgacccggc gcgctggaag atcgaggagc ggggcgagat ccagctcaag
     5221 gggcgcgggc cgctgcagac ttacttcgtc tctcgggcct agcccgcccg gtcctcgcgt
     5281 ccggaggcct gatgttgttc gcggacgacg tcgcacggcc cggggccggt ccggtgcggg
     5341 tgccctgatg tgttcgcgga cgacgtcgca cggcccggtg cgggtgccct gatgtgttcg
     5401 cggacgacga tcacggcccg gggccggtcc ggtgcgggtg ccctgatgtg ttcgcggacg
     5461 acgtcgcacg gcccggggcc ggtccggcgg gtgcccgcgc agcgtctaga aggcaaaaag
     5521 tgaagggggg tccgtcctca aacaggtcgc gctcgtgcgg acatttgggt gcttcgagcg
     5581 agctgtcgct cgcgcttgac tcggccggac cccccttcac tttttgccac acaggccact
     5641 gctaaagggg cgtcggtcgg cgtggccgag agccggaccg gccccggccc tcggggcgcg
     5701 atatgtccgc gcggacatgt cccagcgggc atgctctcgg cgaagcgcac tggaccgcgt
     5761 tcggcccgag cgggcgaccg ctggcgcttg aagatcctac gcgactatct ggtcagacga
     5821 ggttcacgtt cccggcgcag ctcgcctggc tcgcgcttcg ccagccccag gcggcagcgc
     5881 gagcatgccg aagagacatg tccgcatgga catatcgcct gcgcagggcc ggtgcggggc
     5941 cggctctcgg ctccgccgac cgtccggccg tcagtgcagg gtgcgggtgc cggagggccg
     6001 acgggggttt cgaccgagtc cgacgggccc caggacttcg gacgcgaccc aaaacacgcg
     6061 tcgtgtttga gggcgaaacc cccgtcggac atccggctac tcgaccatgc gcgcgcctct
     6121 cagccggacc cgcaccggca cgt
//
LOCUS       DASWNJ010001606         1514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_312022, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001606 DASWNJ010000000
VERSION     DASWNJ010001606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312022"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..427)
                     /locus_tag="VGB85_26675"
     CDS             complement(17..427)
                     /locus_tag="VGB85_26675"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY0137710.1"
                     /translation="MNSASLAHETNTAPGRAALWTGRILSGLVAALLVMSAVAKLGGG
                     PEVEAGFAQAGFKPEHLAPIGVVEIACVVLYLVPWTAFLGAILLTGFLGGAVVTHLRL
                     DDAVHMPILVGVVVWLGLFLRDPRLRRLAPLRSL"
     gene            550..1233
                     /locus_tag="VGB85_26680"
     CDS             550..1233
                     /locus_tag="VGB85_26680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137711.1"
                     /translation="MVPDPSLPLELGFVALTLAVAGLIVVLVRRAGAPTAPVLIGLAI
                     YLTAFFVLGVSGLLADATPPKPLLVLVPALAASLWFALGSRAGEALVAHAPMGALVGL
                     HVFRVPLELMMHAWAQEGALPLQMTFAGINWDIITGVGALLLLPFAERSRIGVLLWNI
                     VGLALLINIVTVAITSLPGPLRLFMNDPPNLLVMRAPYIWLPTFLVQVALAGHILVFR
                     ALRRRRDLP"
BASE COUNT          198 a          574 c          515 g          227 t
ORIGIN      
        1 tgcagcggcg ccgggctcac agcgagcgca gcggcgcgag ccggcgcagc cgcgggtcgc
       61 ggaggaacag gcccagccac acgaccacgc cgacgaggat cggcatgtgc acggcgtcgt
      121 cgaggcgcag gtgggtgacg accgcgccgc cgaggaaacc ggtcagcagg atcgcgccga
      181 ggaacgccgt ccagggcacg aggtagagca cgacgcacgc gatctcgacg acgccgatcg
      241 gggccaggtg ctcgggcttg aagcccgcct gggcgaagcc cgcctccacc tcggggccgc
      301 cgccgagctt ggccaccgcg ctcatcacca gcaacgcggc gaccaggccg ctgaggatcc
      361 gcccggtcca cagcgcggcg cgccccggag cggtgttcgt ttcatgggca agggatgcag
      421 aattcatggg tcctttcgtg gagacggatc cgggcgcctg ctggccccga ggcgcgcatt
      481 gttatcgtcc gcgccggcgc ttcgcaagcg tggacgcccg tgatccggcc ggggtgctat
      541 agggtgatca tggtcccgga tccctcgcta ccgctcgagc tgggctttgt ggccctcacc
      601 ctcgccgtcg ccggcctcat cgtcgtgctg gtgcgccgcg ccggcgcccc gacggccccg
      661 gtgctgatcg gcctcgccat ctacctcacc gcgttcttcg tgctcggggt ctcggggctg
      721 ctcgccgacg ccaccccgcc caagccgctg ctcgtgctcg tgccggcgct ggcggcctcg
      781 ctgtggttcg cgctgggcag ccgcgccggc gaggcgctcg tcgcccacgc gccgatgggc
      841 gcgctggtcg gcctgcacgt gtttcgcgtg ccgctcgagc tgatgatgca cgcgtgggcg
      901 caggagggcg ccctgccgct gcagatgacc ttcgccggga tcaactggga catcatcacc
      961 ggcgtcggcg cgctgctgct gctccccttc gcggagcgca gccggatcgg cgtgctgctg
     1021 tggaacatcg tcgggctcgc gctgctgatc aacatcgtca ccgtcgctat cacctcgctg
     1081 ccggggccgc tgcgcctgtt catgaacgac ccgccgaacc tcctggtgat gcgcgcgccg
     1141 tacatctggc tgccgacctt cctcgtgcag gtcgcgctcg ccgggcacat cctggtgttc
     1201 cgcgcgctgc gtcggcgccg agatctgcca tagctgcgac atgaagactt acaaagcgcg
     1261 cgacgccgac cgcgcagcgc accgtcccac gcgcccggat cgcggcccgg aggcccgcta
     1321 tgccgccgat ctcgcgtgac ctcgcccgat cgccggccga ccccgcttgc ctgcgctgcc
     1381 ggatcgacaa ggctgccacg ccatgacgac cctccttcga cccctcacgt tgatcctcgg
     1441 cctcgccatg atcccggcct gcgccaggac ccgcgccgac aacagcagcc ccgaggagcc
     1501 gggccaggcg tccc
//
LOCUS       DASWNJ010001607         2009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_380124, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001607 DASWNJ010000000
VERSION     DASWNJ010001607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380124"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /locus_tag="VGB85_26685"
     CDS             complement(<1..1452)
                     /locus_tag="VGB85_26685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137712.1"
                     /translation="MRRRWIAGWSLVGVFAAAEPEAYADRDRPDLLVTAPVGPLVAPT
                     AGPHVGSHGGTIVRDEHGLLVVERDEGVVVRAGRDGAAHGRVTLHPGLGEIVGDGLGV
                     VFVADRAADRIVRLSPGDGVGAGLAVAGELAIAEPHGLALTADGATLLVTSVADHQLV
                     AVDTAKWAVRWRVELTTEPRGVAVTRDGSQAIVGFLTTGALAVVDLAPAGPRVRWHSL
                     NPRDTIDVIVEEDMSGGEARAEVSEARSRYQVPASTGKRYARVFPTIALVGGDRVVAP
                     HQISTPQMVRIPDAEQSDRYGGFTQAQPIEQHMALVHRPGVPQAESLLARLDLHQPRA
                     LAYDLASDTLFVGGYGDDGVVAISEVSQQAPYVRWRGTVSRGPRDACGIDGLVLDGER
                     VWVHCALTRRLASIPVARTRRTDSEVKWTVGPQLAEDTRDKLVARGAELFRRGGDFKL
                     SGGGGVMACASCHPEGRSDGLTWRLGPSILQTPM"
     gene            complement(1522..>2009)
                     /locus_tag="VGB85_26690"
     CDS             complement(1522..>2009)
                     /locus_tag="VGB85_26690"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEY0137713.1"
                     /translation="GFPVGTLHPICSLRKELPRGLLDVCSFGIEGDKPARELALRLIG
                     EQPWLDLEGQDAAQRARYHAACALAANYVAVLYDGARSTLREQGHPAEGVDAALAVLM
                     RSALENLLALGIPRGVTGPIARGDQGAADRHAQALGGPAGELYAQLGARLTTMLAAAA
                     G"
BASE COUNT          274 a          822 c          665 g          248 t
ORIGIN      
        1 catcggcgtc tgcaggatcg acggaccgag gcgccacgtc aggccgtcgc tgcggccctc
       61 cgggtggcag ctcgcgcagg ccatcacacc accgccgccg gacagcttga agtcaccgcc
      121 gcgacgaaac agctccgcgc cgcgcgcgac cagcttgtcg cgggtgtcct ccgccagctg
      181 cgggccgacc gtccacttga cctcgctgtc cgtcctccgc gtcctcgcca ccgggatcga
      241 ggccagccgc cgtgtcagcg cgcagtggac ccacacgcgc tcgccgtcga gcaccagccc
      301 gtcgatgccg cacgcgtcgc gcggccccct ggagaccgtc ccgcgccagc gcacgtacgg
      361 cgcctgctgc gagacctccg agatcgcgac caccccatcg tcgccatacc caccgacgaa
      421 cagggtgtca ctcgccaggt cgtaggcgag cgcgcgcggc tgatgcaggt ccaggcgcgc
      481 gagcaacgac tcggcctgcg gcacgcccgg ccggtggacc agcgccatgt gctgctcgat
      541 cggctgggcc tgcgtgaacc cgccgtacct gtcgctctgc tccgcgtcgg ggatccggac
      601 catctgcggc gtcgagatct gatgcggcgc gaccacccga tcgccgccga ccagcgcgat
      661 cgtcgggaac acccgcgcgt atcgcttgcc ggtgctcgcc ggcacctgat acctgctgcg
      721 cgcctcgctg acctcggccc gggcttcgcc gccgctcatg tcctcctcga cgatgacgtc
      781 gatcgtgtcg cgcgggttga gcgagtgcca gcggacccgc gggcccgccg gcgcgaggtc
      841 gacgaccgcg agcgcgcccg tggtcaggaa cccgacgatc gcctggctcc cgtcgcgcgt
      901 caccgcgacg ccgcgcggct cggtcgtcag ctcgacgcgc cagcgcaccg cccacttcgc
      961 ggtgtcgacc gcgaccagct gatgatcggc gacgctggtg accagcaagg tcgcgccgtc
     1021 ggccgtcagc gccagcccgt gcggctcggc gatcgccagc tcgcccgcga ccgccaggcc
     1081 cgcgcccacg ccgtcgcccg ggctcagccg cacgatccgg tccgccgcac gatcggcgac
     1141 gaacaccacc ccgagcccat cgccgacgat ctcgccgagc cccgggtgca gcgtgacacg
     1201 gccgtgcgcc gcgccgtcac gtccggcccg gaccaccacg ccctcgtcgc gctcgaccac
     1261 cagcagaccg tgctcgtcgc ggacgatcgt gccgccgtgg ctgccgacgt gcggcccggc
     1321 cgtcggcgcg acgagtggcc cgaccggcgc cgtcaccagg aggtcgggcc gatcccggtc
     1381 cgcatatgcc tccggctccg ccgccgcgaa caccccgacg agcgaccacc cagcgatcca
     1441 cctgcgacgc atcactggca cagtacgcgc accgcggaaa aagtgttccg ccgcaaatgc
     1501 agcgcgagaa cgcgcgagcg atcagccggc ggccgccgcc agcatcgtcg tcagccgcgc
     1561 cccgagctgg gcatacagct cgcctgcggg cccgccgagc gcctgcgcgt ggcggtccgc
     1621 cgcgccctgg tccccgcgcg cgatcggccc cgtgacgccc cgtgggatcc cgagcgcgag
     1681 caggttctcg agcgccgacc gcatcaacac cgccagcgcc gcgtcgacgc cctcggccgg
     1741 gtgtccttgc tcgcgcaacg tgctgcgcgc cccgtcgtac agcaccgcga cgtagttggc
     1801 cgcgagcgcg caggcggcgt ggtaacgggc ccgctgcgcc gcgtcctggc cctcgaggtc
     1861 gagccacggc tgctcgccga tcaagcgcag cgcgagctcg cgggccggct tgtcgccctc
     1921 gatcccgaag ctacagacat cgagcagccc gcgcggcagc tccttcctca ggctgcagat
     1981 cggatgcagt gtcccgaccg ggaacccgg
//
LOCUS       DASWNJ010001608         1675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_132982, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001608 DASWNJ010000000
VERSION     DASWNJ010001608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132982"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="VGB85_26695"
     CDS             complement(<1..1127)
                     /locus_tag="VGB85_26695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HEY0137714.1"
                     /translation="MPKHSPRPAPRLRPPVARLCHWLPAASVLVGALVGCGPSPDPYM
                     IDRDTAIAENRFLRAIHSPEAVEDEEPPSEDQENTDEDAAGAGQRHRGEEGKMGRPTS
                     KMKSGLYAMKGPRDAIPQMARSFDGADCFGCETGDDKYGRPADNQWAQVVAEPLSTFS
                     IDVDTASYSNVRRYLQGGSLPPPDAVRTEELINYFDYDYAQPSDGRPLAVHAEVAPCP
                     WNSTHKLVHIGMQGKVVPAAEIGPRNLVFLVDVSGSMGAPDKLPLLRAGLRMLVDDMD
                     ADDRISMVAYAGASGLVLPPTSGADKNTILHAINNLESGGSTNGGAGIALAYAQARKH
                     LIKGGVNRVILASDGDFNVGVTDHDELVRLVEQQRESGVFL"
     gene            complement(1265..>1675)
                     /locus_tag="VGB85_26700"
     CDS             complement(1265..>1675)
                     /locus_tag="VGB85_26700"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0137715.1"
                     /translation="TLEPRTLSVGDLDGDGELDIVVASYTYPNLNVLIGQGDGSFADQ
                     PGFVPSKVGLYAVVAVDLDHDGALDIVGVDRDAHALLVIRNIGPGKISTEDVYPLGLH
                     PLDLLVVDLDGDGARDVVTADSTSGAVTVLRANP"
BASE COUNT          242 a          551 c          625 g          257 t
ORIGIN      
        1 ctcaggaaca cgccgctctc gcgctgctgt tcgaccagcc gcacgagctc gtcgtggtcg
       61 gtgacgccga cgttgaagtc gccgtccgag gcgaggatga cccggttgac gccacccttg
      121 atcaggtgct tgcgggcctg cgcgtaggcg agcgcgatgc cggccccgcc gttggtcgac
      181 ccgccggact cgaggttgtt gatcgcgtgg aggatcgtgt tcttgtcggc gccggaggtc
      241 ggcggcagca cgaggcccga ggcgccggcg taggcgacca tcgagatgcg gtcgtcggcg
      301 tccatgtcgt cgacgagcat gcgcagacca gcgcgcagca gcgggagctt gtcgggcgcg
      361 cccatcgaac ccgagacgtc gacgaggaac acgaggttgc gcggcccgat ctcggcggcc
      421 ggcacgacct tgccctgcat gccgatgtgc acgagcttgt gcgtgctgtt ccacgggcac
      481 ggcgcgacct cggcgtggac cgcgagtgga cggccgtcgc tcggctgggc gtaatcgtaa
      541 tcgaagtagt tgatcagctc ctcggtgcgc accgcgtccg gcggcggcag cgagccgccc
      601 tgcaggtagc ggcgtacgtt gctgtagctg gcggtgtcga cgtcgatcga gaaggtcgac
      661 agcggctcgg cgacgacctg cgcccactgg ttgtcggcgg gccgcccata cttgtcatcg
      721 ccggtctcac agccgaagca gtccgcgccg tcgaagctgc gcgccatttg tgggatcgcg
      781 tcgcgcgggc ccttcatcgc gtagaggccc gacttcatct tcgacgtcgg gcggcccatc
      841 ttgccctcct cgccgcgatg gcgctggccc gcgccggcgg cgtcctcgtc ggtgttctcc
      901 tggtcctccg acggcggctc ctcgtcctcg accgcctccg ggctgtggat cgcccgcagg
      961 aagcggttct cggcgatcgc ggtgtcgcgg tcgatcatgt aggggtccgg cgaggggcca
     1021 cagccgacca gcgcgccgac gagcacgctc gcggccggca gccagtgaca caggcgggcg
     1081 acgggaggac gaaggcgagg agcggggcgc ggcgagtgct tgggcatggc gtcggctcca
     1141 gacatgcgag ccccacgaga gttccgcggc gccgcgagat ttgccgaacg caccccgcgg
     1201 ggagcgacgt acggacaaat cgccgagacg cagggaacct cgccgccggt gcgctgtcgg
     1261 ccgctcaggg gttcgcgcgc aggacggtga cggcgcccga ggtcgagtcc gcggtgacga
     1321 cgtcgcgcgc gccgtcgccg tcgagatcga cgacgagcag gtcgagcggg tggaggccga
     1381 gcggataaac atcctcggtc gagatcttgc cgggcccgat gttgcggatc acgagcagcg
     1441 cgtgcgcgtc gcggtcgaca ccgacgatgt cgagcgcgcc atcgtggtcg aggtcgacgg
     1501 cgacgaccgc gtacaggccg accttcgagg gtacaaagcc cggctggtcg gcgaagctgc
     1561 cgtcgccctg accgatcagc acgttgaggt tcggataggt gtagctggcg acgacgatgt
     1621 cgagctcgcc gtcaccgtcg aggtcgccga cggagagcgt gcgcggctcc agcgt
//
LOCUS       DASWNJ010001609         6089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_594322, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001609 DASWNJ010000000
VERSION     DASWNJ010001609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594322"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VGB85_26705"
     CDS             complement(<1..446)
                     /locus_tag="VGB85_26705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137716.1"
                     /translation="MDRTHRARIALGLALALAGPAACGDPAPSGDGDSEHAGTGGAAS
                     SEPDPTTGASNPALTGESGEAGESGSGGAEGDDSGEDSGPAVPACDPEALPIGEREFI
                     PRGESRSDVVLAPCSEDRWYFSAASESTVEVTLRRRDGGAVAAAIA"
     gene            505..1242
                     /locus_tag="VGB85_26710"
     CDS             505..1242
                     /locus_tag="VGB85_26710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEY0137717.1"
                     /translation="MDVTASTKLFPDVAAALADLAEGASIAVGGFGLCGNPEACIRAI
                     AARGTLRLTIISNNCGNQGQGLAVLLKQRQVARVVCSFVGGNPDLEQQMLAGEVEVEL
                     NPQGTLAERLRAGGAGIPAFFTPTGAGTVVAEGKEVRDFEGRPCVLETALRPDFAIVR
                     AARGDALGNLQFYRTARNFNPLAAMAGRITIAEVDELVPIGALDPDDVHLPGIFVQRV
                     VHVPEHADIIEFRTTRPRPAGDPPRAE"
     gene            1396..1995
                     /locus_tag="VGB85_26715"
     CDS             1396..1995
                     /locus_tag="VGB85_26715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family glyoxalase III"
                     /protein_id="HEY0137718.1"
                     /translation="MTSDDMIDEVEEVETTIPRALVILAEGAEEAEAVIIVDVLRRAG
                     LETVFASLGGEAVVQCSRKVRIATDSPLAGVTGNFDVIILPGGAEGARRLCASEAVGE
                     RLKNQSREGKLVAAICAAPLAFQAHQVFAGRTMTCHPSVASRIAMYGKLERAAVVEDG
                     NLITSQGPGTAFVFALAVVRRVMGEPRAAEVERGLVLMK"
     gene            complement(2064..2999)
                     /locus_tag="VGB85_26720"
     CDS             complement(2064..2999)
                     /locus_tag="VGB85_26720"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEY0137719.1"
                     /translation="MGPLLAVLLGALQGVAEFLPISSSGHLALAQTWLGVDPAAGGHT
                     FNIVVHAGTLLAVLMFYRDDLVALVRAVFQRQQVPIVHHETTRAAPAPEPLAIAEDLA
                     DGPGVRPAPPVDVLPNRVGPPINTARALLYALIVATLPLVFALTRTVENIVVTAEGST
                     RWIGCGFLLTAALLGFSHRRSDAGLLEAPRPWQGLVIGLAQLVAILPGISRSGSTMAA
                     AMALGLGRLPAARFSFLLSIPAILGASLKETIDLARSDAALDIEFTSFALGFAASFAV
                     GTLTLRALMRLLVRVGLLPFVPYLIVLGAVVVATS"
     gene            complement(3014..4984)
                     /gene="hflX"
                     /locus_tag="VGB85_26725"
     CDS             complement(3014..4984)
                     /gene="hflX"
                     /locus_tag="VGB85_26725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY0137720.1"
                     /translation="MSSEIVHGDTANLKSSQARQLQRFGARQVPPQLVISAVLARDLI
                     DLSQVLNRQLGLFIDRRGRVQRVILGDAHSLALPEFTRVRGQGGRLRGIRLVVTHLVP
                     DPLTREELADLAKLRLDLVAAIHRGPAGVQIDLASLAPAAPTSNDVFVVRTFPRVPLG
                     VLSRDDDEDHPLPAPDMPADFLDYIGDLEAGLVAATQDARAESQGIRAMALMIHQGAG
                     QELAAREQELRELCRTAGLHLVDLATQRRPGPDPRTFFGQGKLRDVLVRALAQDVELL
                     ICDPDLTPSQAREIGEHTDLKVIDRTTLILDIFAQHARSSDGKLQVELAQLRYLLPRM
                     IGHGTAMSRLAGGIGGRGPGETKLEIDRRRAKERIHDLERRLDMLKQRRDQRRALRRR
                     TGVPVVAIVGYTNAGKSSLLNTVTESAVIAEDKLFATLDPTVRRIRFPKDYEVVMLDT
                     VGFIRDLPPALVQAFSATLEEVAEADLLLVVVDASDPDQDQQRATVEAILGDIGAGAV
                     PRLWVYNKADRLEPEDLERRRADAGRDTYFLSALDRRSTAALMQGIDEHLWERGVLDR
                     KTVPEDMSQTPDEADEPDTYAPDAPGGADEYDDPDAPDPRATAEREVSDEPDDADEPD
                     NPDAPDDLDGVAAPDEPDASDEPAGPDKDGAS"
     gene            complement(4981..>6089)
                     /locus_tag="VGB85_26730"
     CDS             complement(4981..>6089)
                     /locus_tag="VGB85_26730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137721.1"
                     /translation="LNAKNREILALKDELEARERAVLDAKKHHRELQAQLGELETQLV
                     GSQEQILSAREAAESAQRDKQTILKREEGLKTRLEVIQKKLKDTEQQLSTAQSQLQAA
                     QQQATAAEARASQQAATITALEAERDGLATRVQALDEHVTGLEATIAERERTHAELER
                     THAELTGQLEQVRQELSDSIASAAEAQAAAAEAHAAALAALGEQHQTEIDEREADHRG
                     VIEALQAQLAELTEQATQERERMNQALQETEDNARQQIAQLTQHLASTEESARSEIQR
                     VTQNLAAAEAHARAEIQRLQSEAEQVSVQAQADAEDASRKIHELEATLDELSGSLDRA
                     EASLHRRREAALHAQQAIAVALRVLDGSSDSDSP"
BASE COUNT          756 a         2008 c         2421 g          904 t
ORIGIN      
        1 taggcgatcg cggccgccac cgcgccgccg tcgcggcgac gcagcgtgac ctcgacggtg
       61 ctctcgctcg cggccgagaa gtaccagcgg tcctcgctgc acggcgcaag cacgacatcg
      121 gagcgcgact cgccgcgcgg aatgaactcg cgctcgccga tcgggagcgc ctccggatcg
      181 caggcgggca ccgccgggcc gctgtcctca ccgctgtcgt cgccctcggc gcccccgctg
      241 ccggactcgc cggcctcgcc ggactcgccg gtcagcgccg ggttcgaggc gccggtcgtc
      301 gggtcgggct cgctgctcgc tgcaccgcca gtgccggcgt gctcgctgtc gccgtcgccg
      361 gacggcgccg ggtcaccgca tgcggccggc cccgcgagcg cgagcgcgag gccgagtgcg
      421 atccgtgcgc ggtgggtgcg atccatgcga ggcgcacgat agccgagcgt gcgggcgccg
      481 agaagatccg gtaaaacgcc ggccatggac gtgaccgcca gcaccaagct gttccccgat
      541 gtcgcagcgg ccctcgcgga cctcgccgag ggggcgtcga tcgcggtcgg cggctttggt
      601 ctgtgtggca acccggaggc ctgcatccgc gcgatcgcgg cccgcggcac cctgcgcctg
      661 acgatcatct cgaacaactg cggcaatcag gggcagggtc tggcggtcct gctcaagcag
      721 cggcaagtcg cccgcgtcgt gtgcagcttc gtcggcggca accccgacct cgagcagcag
      781 atgctcgccg gcgaggtgga ggtcgagctc aacccgcagg gcacgctcgc cgagcgcctg
      841 cgggccggcg gcgccggcat cccggcgttt ttcacgccga ccggcgcggg cacggtggtc
      901 gcggagggca aggaggtgcg tgacttcgag ggccggccct gcgtgctcga gacggcgctg
      961 cggcccgact ttgcgatcgt gcgagccgcc cgcggcgacg cgctcggcaa cctccagttt
     1021 tatcgcaccg cccgcaactt caacccgctg gcggcgatgg ccggccggat cacgatcgcc
     1081 gaggtggacg agctcgtccc gatcggcgcg ctcgaccccg acgacgtcca cctgccgggg
     1141 atcttcgtcc agcgcgtcgt ccacgtcccc gagcacgccg acatcatcga gtttcggacg
     1201 acccggccgc gacccgcggg cgatccgccg cgcgccgagt gatccgtaga aacgcggttc
     1261 gtgggccaga tttcgtcgat ccttgcgatg cgcgcctccg cgcgtgcggc gtgcggtacc
     1321 ctgtgtgtcc ggttcttggc cctgtggcgc gttcgcaggt agcctcgagg gccgagaagg
     1381 agcgctggag gttttatgac gagcgacgac atgatcgacg aggtcgaaga ggtcgagacg
     1441 acgatccctc gcgccctcgt catattggcc gagggtgccg aggaggcgga ggcggtgatc
     1501 atcgtcgatg tgctgcgccg cgccggcctc gagaccgtgt tcgcctcgct cggcggcgag
     1561 gcggtggttc agtgcagccg caaggtccgc atcgccaccg atagcccgct cgccggtgtg
     1621 accggcaact tcgacgtcat catcctgccc ggcggtgccg agggggcccg ccgcctgtgc
     1681 gcgtcggagg cggtcggcga gcgcctcaag aatcagagcc gcgagggcaa gctcgtcgcg
     1741 gcgatctgcg ccgcaccgct ggccttccag gcccatcagg tgttcgccgg gcgcacgatg
     1801 acctgccacc cctcggtcgc gtcgcgcatc gcgatgtacg gcaagctcga gcgcgccgcg
     1861 gtcgtcgagg acggcaacct gatcaccagc cagggacccg gcaccgcctt cgtgttcgcg
     1921 ctcgcggtgg tccgcagggt gatgggtgag ccgcgcgcgg ccgaggtcga acgtgggctc
     1981 gtgctcatga agtgagacgc atggtgtgcc cgggcggcgt gcgcccgggc accgcggcag
     2041 gctcgcacgc gtggtcggcg agctcaggag gtagcgacga cgaccgcgcc gagcacgatc
     2101 aggtacggca cgaagggcag caggccgacg cggacgagca ggcgcatgag ggcgcgcagg
     2161 gtcagggtgc cgacggcgaa gctggcggcg aagccgagag cgaagctcgt gaactcgatg
     2221 tcgagcgcgg cgtcggagcg ggcgaggtcg atcgtctcct tgagcgaggc gccgaggatc
     2281 gccgggatcg agagcaggaa ggagaagcgg gcggcgggca ggcggccgag gccgagcgcc
     2341 atcgcggcgg ccatggtcga cccgctgcgc gaaatgccag gcaggatcgc gacgagctgc
     2401 gcgaggccga tgacgaggcc ctgccagggg cgcggggcct cgagcaggcc ggcgtcgctg
     2461 cggcggtgcg agaagcccag cagggcggcg gtcagcagga agccgcagcc gatccagcgg
     2521 gtgctgccct cggcggtgac gacgatgttc tcgacggtgc gggtcagcgc gaacaccagc
     2581 ggcaaggtgg cgacgatcag cgcgtagagc agggcgcggg cggtgttgat cggcgggccg
     2641 acgcggttcg ggagcacgtc gaccggcggc gcgggccgga caccggggcc gtcggcgagg
     2701 tcctcggcga tcgccagcgg ctcgggggca ggggccgccc gggtcgtctc gtggtggacg
     2761 atcgggacct gctggcgctg gaacacggcg cgcacgaggg cgacgaggtc gtcgcggtag
     2821 aacatcagca cggcgagcag ggtgccggcg tggacgacaa tgttaaaagt gtggccgccc
     2881 gcggccgggt cgacgccgag ccaggtctgc gcgagcgcga ggtgacccga cgacgagatc
     2941 ggcaggaact cggcgacccc ctggagggcg ccgagcagca cggcgagcag cggtcccatc
     3001 gcgggatccg acatcacgac gctccgtctt tgtcgggtcc tgcgggctcg tcggaggcgt
     3061 ccggctcgtc gggcgcggcg accccgtcca gatcgtcggg cgcatcgggg ttatcgggct
     3121 cgtcggcgtc gtcgggctcg tcggacacct cccgctcggc ggtcgcgcgc gggtcggggg
     3181 cgtcgggatc gtcgtactcg tcggccccgc cgggagcgtc gggcgcgtag gtgtcgggct
     3241 cgtcggcctc gtcgggcgtt tgggacatgt cctcggggac agtctttcgg tcgagcacgc
     3301 cgcgctccca caggtgctcg tcgatgccct gcatcagcgc ggcggtgctg cggcggtcga
     3361 gggcgctgag gaagtaggtg tcgcggccgg cgtcggcgcg gcggcgctcg aggtcctccg
     3421 gttcgaggcg gtcggccttg ttgtagaccc acaggcgggg cacggcgcct gcgccgatgt
     3481 ctccgaggat cgcctcgacg gtggcgcgct gctggtcctg gtcggggtcg ctggcgtcga
     3541 cgacgacgag cagcaggtcg gcctcggcga cctcctcgag ggtggcgcta aacgcctgca
     3601 ccagcgcggg ggggaggtcg cggatgaagc cgacggtgtc gagcatcacg acctcgtagt
     3661 ccttggggaa gcggatcctg cggacggtcg ggtcgagggt ggcgaacagc ttgtcctcgg
     3721 cgatcacggc cgactcggtg acggtgttga gcaggctgct cttgccggcg ttggtgtagc
     3781 cgacgatcgc gacgacaggc acgccggtgc gccggcgcag ggcccgacgc tggtcgcggc
     3841 gctgcttgag catgtcgagg cggcgctcga ggtcgtggat gcgctccttg gcgcggcggc
     3901 ggtcgatctc cagcttggtc tcgccggggc cgcgcccgcc gatgccgccg gcgagccgcg
     3961 acatcgcggt gccgtggccg atcatgcgcg gcagcaggta ccgcagctgg gcgagctcga
     4021 cctgcagctt gccgtccgag gagcgggcgt gctgggcgaa gatgtcgagg atgagggtcg
     4081 tgcggtcgat gaccttgagg tcggtgtgct cgccgatctc acgcgcctgc gagggggtca
     4141 gatcggggtc gcagatcagc agctcgacgt cctgcgcgag cgcccgcacg agcacgtcgc
     4201 gcagcttgcc ctgaccgaag aaggtgcgcg ggtcggggcc ggggcggcgc tgggtcgcga
     4261 ggtcgacgag gtgcaggccg gcggtgcggc acagctcgcg cagctcctgc tcgcgcgcgg
     4321 cgagctcctg accggcgccc tggtggatca tcagggccat cgcgcggatc ccctgcgact
     4381 cggcgcgcgc gtcctgggtc gcggcgacga ggccggcctc gaggtcgccg atgtagtcga
     4441 ggaagtcggc cggcatgtcc ggcgcgggca gggggtggtc ctcgtcgtcg tcgcggctga
     4501 gcacgccgag cggcacgcgc gggaaggtgc gcacgacgaa cacgtcgttg ctggtcggcg
     4561 cggccggggc gaggctggcg aggtcgatct gcacgccggc cgggccgcgg tggatcgcgg
     4621 cgacgaggtc gaggcgcagc ttggcgaggt cggcgagctc ctcgcgcgtc agcgggtcgg
     4681 gcacgaggtg cgtgacgacg agccgaatgc cgcgcagacg gccgccctga ccgcgcacgc
     4741 gcgtgaactc gggcagggcc aggctgtgcg cgtcgccgag gatcacgcgc tggacccggc
     4801 cgcggcggtc gatgaacagg ccgagctggc ggttgagcac ctgtgagagg tcgatcaggt
     4861 cgcgcgcgag cacggccgag atgaccagtt gtggcgggac ctgacgcgcg ccgaagcgct
     4921 ggagctgccg ggcctggctg ctcttgaggt tcgccgtatc gccgtggacg atctcggagc
     4981 tcacggtgag tcgctgtcgc tgctgccgtc gaggacgcgg agggccaccg cgatcgcctg
     5041 ctgggcgtgc agggcggcct cgcggcggcg gtgaagggat gcctcggcgc ggtcgaggct
     5101 gccgctgagc tcgtcgaggg tcgcctcgag ctcgtggatc tttcggctgg cgtcctcggc
     5161 gtcggcctgg gcctgcacgc tgacctgctc ggcctccgac tgcaggcgct ggatctcggc
     5221 gcgggcgtgg gcctcggcgg cggcgaggtt ctgggtgacg cgctggatct cgctgcgggc
     5281 gctctcctcg gtcgaggcga ggtgctgcgt cagctgggcg atctgctggc gggcgttgtc
     5341 ctcggtctcc tgcagggcct ggttcatgcg ctcgcgctcc tgcgtggcct gctcggtcag
     5401 ctcggcgagc tgggcctgca gggcctcgat gacgccgcgg tgatcggcct cgcgctcgtc
     5461 gatctcggtc tggtgctgct cgccgagagc agcgagggcg gcggcgtggg cctccgcggc
     5521 ggcggcctgc gcctcggcgg cggaggcgat gctgtcgctc agctcctggc ggacctgctc
     5581 gagctggccg gtcagctcgg cgtgggtacg ctcgagctcg gcgtgggtcc gctcgcgctc
     5641 ggcgatcgtg gcctcgaggc cggtgacgtg ctcgtcgagg gcctggacgc gggtggcgag
     5701 gccgtcacgc tcggcctcga gggcggtgat cgtggcggcc tgctggctcg cgcgggcctc
     5761 ggcggcggtc gcctgctgct gggcggcctg gagctgggac tgggcggtcg acagctgctg
     5821 ctcggtgtcc ttgagcttct tctggatcac ctcgaggcgg gtcttgaggc cctcctcgcg
     5881 cttgaggatc gtctgcttgt cgcgctgggc cgactcggcg gcctcgcgcg cggacaggat
     5941 ctgctcctgc gagccgacga gctgggtctc gagctcgccg agctgagcct gcagctcgcg
     6001 gtggtgcttc ttggcgtcga gcacggcgcg ctcgcgggcc tcgagctcgt ccttgagggc
     6061 gaggatctcg cggttcttgg cgttgagct
//
LOCUS       DASWNJ010001610         1625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_522017, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001610 DASWNJ010000000
VERSION     DASWNJ010001610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522017"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1625
                     /gene="tssI"
                     /locus_tag="VGB85_26735"
     CDS             <1..>1625
                     /gene="tssI"
                     /locus_tag="VGB85_26735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HEY0137722.1"
                     /translation="QDRSAPEVLKAVLSDALAPYRRSVRIDLGTEYPRREYCTQFRES
                     DLDFAARLMEEEGISYFFEHEGGVEVLVLVDDNARFPTLSGRADSDAVPVLENASETL
                     RLESVQTFMWSRKLHSTSAVQRDFDFLRPDLAQSHEERGTDEAGRDREVYVHDDLLFM
                     NDGARRARLRREQLGGAGKVARGTSNATTFSPGLKFRLVDHRRGDLDRGYVLTRVTHT
                     GECPDALIEVRRAEAGPRYTNEFECVPDDVAVRPAPRHSKPRVHGPTTATVVGPASEE
                     VHTDEHGRVKVLFPWDRLSPMDDTASWWVRVTQGWAGPGWGALFLPRIGMEVVVEFLD
                     GDPDRPLVTGCVYNGRNPPPYALPADKTKSGLKSSNSPSDGGFNELRFEDQKGVEEVF
                     IHAERDLNTVVKRSKSLTVGASESNTVGENRDVKVAMNDALAVGMNRAETVGMNRALT
                     VAMNSAIAVGANQSETIAISASRTVGAGYQVSVGGPMTESVGESKTETIADFKSVSTG
                     RSSTEEVGEDKLVHVADDSVETVGGDKSIDAGD"
BASE COUNT          270 a          485 c          648 g          222 t
ORIGIN      
        1 gcaggaccgc agcgcgccgg aggtgctcaa ggcggtgctc tcggacgcgc tggcgccgta
       61 tcgccggtcg gtgcggatcg acctgggcac ggagtacccg cggcgcgagt actgcacgca
      121 gtttcgcgag tcggacctcg acttcgcggc gcggctgatg gaggaggagg gcatctcgta
      181 cttcttcgag cacgagggcg gggtcgaggt gctggtgctg gtcgacgaca acgcccgctt
      241 cccgacgctg tcgggccggg cggacagcga cgcggtgccg gtgctcgaga acgcgtcgga
      301 gacgctgcgg ctcgagtcgg tgcagacgtt catgtggtcg cgcaagttgc actcgacctc
      361 ggcggtgcag cgtgacttcg acttcctgcg tccggacctg gcgcagagcc acgaggagcg
      421 cggcacggac gaggcggggc gcgaccgcga ggtgtacgtg cacgacgacc tgctgttcat
      481 gaacgacggt gcgcggcggg cgaggctgcg gcgcgagcag ctcggcgggg cgggcaaggt
      541 ggcgcgcggt acgagcaacg cgacgacgtt ctcgccgggc ctgaagtttc gcctggtcga
      601 ccaccggcgc ggcgacctcg atcgcgggta cgtgctgacg cgggtgacgc acaccggcga
      661 gtgcccggac gcgctgatcg aggtgcggcg cgcggaggcc gggccgcgct ataccaacga
      721 gttcgagtgc gtgcccgacg acgtcgccgt gcgaccggcg ccgcggcaca gcaagccgcg
      781 ggtgcacggg ccgacgacgg cgacggtggt cggcccggcg agcgaggagg tgcacaccga
      841 cgagcacggg cgcgtcaagg tgctgttccc gtgggaccgg ctgtcaccga tggacgacac
      901 ggcgtcgtgg tgggtgcgcg tgacgcaggg ctgggctggg ccgggctggg gcgcgctgtt
      961 cttgccgagg atcggtatgg aggtggtcgt cgaattcttg gacggcgatc cggaccggcc
     1021 gctggtgacc ggctgcgtgt acaacggccg taatccgccg ccttatgcgc ttccggccga
     1081 caagaccaag agcgggctca agtcgagcaa ctcgccgtcc gacggcggct tcaacgagct
     1141 gcgcttcgag gatcagaagg gcgtcgagga ggtctttatt catgccgagc gcgacctcaa
     1201 cacggtcgtc aagcgaagta aatcgctgac ggtcggggcg agcgagagca atacggtcgg
     1261 cgagaaccgc gacgtcaagg tggcgatgaa cgacgcgctg gcggtcggga tgaaccgcgc
     1321 cgagacggtc gggatgaacc gcgcgctgac ggtggcgatg aacagcgcga tcgcggtcgg
     1381 ggccaaccag agcgagacga tcgcgatctc ggcgtcgcgg accgtcgggg cggggtatca
     1441 ggtgtcggtc ggcggaccga tgacggagag cgtcggcgag agcaagaccg agacgatcgc
     1501 ggacttcaag tcggtgtcga ccgggcgcag cagcacggag gaggtcggcg aggacaagct
     1561 ggtccacgtc gccgacgaca gcgtcgagac ggtcggcggc gacaagagca tcgacgccgg
     1621 tgaca
//
LOCUS       DASWNJ010001611         4675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_168418, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001611 DASWNJ010000000
VERSION     DASWNJ010001611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168418"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VGB85_26740"
     CDS             <1..364
                     /locus_tag="VGB85_26740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137723.1"
                     /translation="VPTSLLALALAGHEQALSVLLGVAIGLFNFQSLARAAVRILLTA
                     EAEPDATAASTSRFAPLLAMLRWPATALATAAVLWYMPGRPEGLVVGVLVALVGFTAA
                     ALQSAGEISRPTPDDTDE"
     gene            427..1182
                     /gene="atpB"
                     /locus_tag="VGB85_26745"
     CDS             427..1182
                     /gene="atpB"
                     /locus_tag="VGB85_26745"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY0137724.1"
                     /translation="MPDFWAQLEHGAAEHLAREQKFMMFQGTHFTLVHVAGALLAFIF
                     ILILAIRYRASLKDETRGVVPPRKFGLAAMTDGFVGAVYNLSVDVMGEKNARKYLPLT
                     GTLALFIFVCNIQGLVPGLIPPTDTLKTNLVFALIVFVVYNVAGVLNNGLSYLAHFLG
                     PKIGGFPWLFPLFLPIEIVSHLARPVSLSLRLTGNIVADHKVVAVVAMMVPLLVPLPF
                     LLLGTVVAIVQTLVFTLLAIIYIGLAVEHAEEH"
     gene            1263..1598
                     /locus_tag="VGB85_26750"
     CDS             1263..1598
                     /locus_tag="VGB85_26750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEY0137725.1"
                     /translation="MTRNVAKLATFLFVFLAPSIVLAAGAETNGGERSVYGFAMALAV
                     GLAALGGGMGQGRAVAAALEGICRNPNSAGKVLVPMLLGLAFIESLVIFALIIAFTIG
                     GYIAPTIGG"
     gene            1755..3218
                     /locus_tag="VGB85_26755"
     CDS             1755..3218
                     /locus_tag="VGB85_26755"
                     /inference="COORDINATES: protein motif:HMM:NF024209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEY0137726.1"
                     /translation="MRLALRATLLALSACGPTGELAEATAGPDSGSSGTTGDPPAPTS
                     EPTTTGEAMTGGATTGTTVDLTTGSTTDATTGTTGGSSTDASSSSTGEPAIDCTELAI
                     ADPVILAEIRKQLEIPEGPISGEAALAVKSLYLNDGPIATLAGVKCLTDMHTLQGSGP
                     VSDLGPLAGLKSLVNLALRDSEVTDLGPLVGLPLDTLVLPRSPLADLGPLAQIPTLDT
                     LVIASTSVQDLTPLTGHPGLESITADETLPLDLLPLATIPKLTRLSMEACSLDDVSAL
                     AGATALQGLYIDDNALVDLDGLATLTQLRSLLASGNQITSVAPLSGLDKLEDLRLANN
                     KISDLKPLAGATALKTLIVSSNPLAGLQGLEALPQLDTLTAETAGLKALVTVAPNTLI
                     GLHLNGNQINDVTPLAGNQFLGFVHLDDNAITTLAPIQQAPWVQSHCANLQVSGNPLD
                     AATLATTLPAMCDLDMTIAWTQGECAPDGFRCGFSDP"
     gene            complement(3335..4273)
                     /gene="trxB"
                     /locus_tag="VGB85_26760"
     CDS             complement(3335..4273)
                     /gene="trxB"
                     /locus_tag="VGB85_26760"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEY0137727.1"
                     /translation="MRETIHSKVLILGAGPAGYTAAIYAARANLEPIMVCGPEPGGQL
                     TTTTEVENYPGFPKGITGPEMMDEFREQAERFGTKLYYDLGLEADLSKRPFTIVGESA
                     VYTGDTLIIATGASAKWLGLPNEQRLRLSGGGVTACATCDGAFYRDQDVVVIGGGDTA
                     MEEATFLTRMCRSVHMIHRRDSFRASKIMQKRVFENPKITVHWNREVVDVLGTDRVVG
                     VRLQDTVNGTQEDLAVTGVFLAIGHNPNSPIFKGQLDMDELGYLKTIPGTAATRVPGV
                     YACGDVADKVYRQAITAAGTGCMAAIEAERWMAEHG"
BASE COUNT          708 a         1765 c         1462 g          740 t
ORIGIN      
        1 cgtgccgacc tcgctcctgg ccctcgcgct cgccggccac gagcaggcgc tcagcgtgct
       61 gctcggcgtg gcgatcggcc tgttcaactt tcaatcgctg gcccgcgcgg ccgtccgcat
      121 cctgctgacc gccgaggccg agccggacgc gaccgccgcc agcaccagcc gatttgcgcc
      181 gctgctcgcc atgctgcgct ggccggccac tgcccttgcg acggctgccg tcctctggta
      241 catgcccggc cgacccgagg ggctggtggt gggggtgctg gtcgccctcg tcggtttcac
      301 cgccgcggcg ctgcaaagcg ctggtgagat ctcccgcccc acgcccgacg acaccgacga
      361 gtagccgact ccttccttcc ggaccgccga cctatgggtg accacgactc ctggtacacg
      421 ctcctgatgc ccgacttctg ggcccagttg gagcatggtg ccgccgagca tctggcgcgt
      481 gaacagaagt tcatgatgtt ccaggggacc cacttcaccc tggtgcacgt cgccggggcg
      541 ctgctggcct tcatcttcat cctgatcctc gccatccgtt accgggcgag cctcaaggac
      601 gagaccaggg gcgtcgtccc gccgcgcaag ttcggcctcg ccgcgatgac cgacggcttc
      661 gtcggcgccg tctacaacct ctccgtcgac gtgatgggcg agaagaacgc ccgcaagtac
      721 ctgccgctga ccggcacgct cgcgctgttc atcttcgtct gcaacatcca gggcctcgtc
      781 cccggcctga tcccgccgac cgacacgctc aagaccaacc tcgtcttcgc gctgatcgtg
      841 ttcgtcgtct acaacgtcgc cggcgtcctc aacaacggcc tgagctacct cgcccacttc
      901 ctcggcccga agatcggcgg cttcccctgg ctgttcccgc tgttcctgcc gatcgagatc
      961 gtcagccacc tggcccgccc ggtgtccctg tccctgcgcc tcaccggcaa catcgtcgcc
     1021 gaccacaagg tcgtcgccgt cgtcgccatg atggtccccc tcctcgtgcc gctgcccttc
     1081 ctgctgctcg gcaccgtcgt cgccatcgtc caaaccctcg tcttcacgct cctcgccatc
     1141 atctacatcg gcctcgcggt cgaacacgcc gaagagcact gaccttcgtc cgacttccgc
     1201 agcgctccgc gccgcatcaa aacaccgtct ccggcgctgc gcgccgcctt gaggaacacc
     1261 ccatgacccg caacgtcgcc aagctcgcca ccttcctgtt cgtcttcctc gccccctcca
     1321 tcgtcctcgc cgccggcgcc gagaccaatg gtggtgagcg ctcggtctat ggcttcgcca
     1381 tggcgctcgc cgtcggcctc gccgccctcg gcggcggtat gggccagggc cgcgccgtcg
     1441 cggccgccct cgagggcatc tgccgcaacc cgaactcggc cggcaaggtc ctcgtcccga
     1501 tgctcctcgg cctcgccttc atcgagtccc tggtcatctt cgccctcatc atcgccttca
     1561 ccatcggcgg ctacatcgcc ccgacgatcg gcggctgagc cgaccccgca cgccgcgagc
     1621 gtgcgtggcg acgatataac gcccacaggc gctccggctg tccaaacagc cagagcgcct
     1681 ttgcgcgttc aggtcatgac aaaagtatgg gggaggccac ccccgcaggt ctgcatcggt
     1741 gagagcatgc cgggatgcgc cttgcccttc gtgcgaccct cctcgccctc tccgcctgcg
     1801 gtcccacggg ggagctcgcc gaggcgaccg ccggtccgga ctccggctcg agcggcacga
     1861 ccggcgaccc gccggcgccg accagcgagc ccacgacgac cggcgaagcg atgaccgggg
     1921 gagcgacgac cggcacgacc gtcgacctga cgaccggcag cacgacggac gcgaccacgg
     1981 gcacgaccgg tggatcgagc acggacgcat cgagctcgag cacgggcgag ccggcgatcg
     2041 actgcacgga gctggcgatc gccgatcctg tgatcctcgc cgagatccgc aaacagctcg
     2101 agatccccga gggcccgatc tctggcgagg ccgcgctcgc ggtgaaatcc ctctacctca
     2161 acgacggccc gatcgcgacg ctcgccggag tcaagtgcct gacggacatg cacacgctgc
     2221 agggcagcgg acccgtcagc gacctcggcc cgctggcggg cctcaagagc ctggtgaacc
     2281 tggccctgag ggacagcgaa gtcaccgacc tcggcccgct cgtcggcctg ccgctcgaca
     2341 ctctcgtgct gccgcgctcg ccgctcgccg acctcggccc actcgcgcag atccccacgc
     2401 tcgacacgct cgtgatcgcc tcgacgtccg tccaggacct caccccgctg accggccatc
     2461 ccggtctcga gtcgatcacc gccgacgaga cgctcccgct cgacctgctg ccgctcgcca
     2521 cgatccccaa gctgacgcgg ctctcgatgg aggcgtgcag cctcgacgat gtcagcgcgc
     2581 tggcgggggc gaccgcgctg caggggctgt acatcgacga caacgcgctc gtcgatctcg
     2641 atggcctcgc caccctgaca cagctccgtt ccctgctcgc gtcgggcaac cagatcacga
     2701 gtgtcgcgcc gctgtcgggc ctcgacaagc tcgaggacct gcgcctcgcc aacaacaaga
     2761 tctcggacct caagccgctg gcgggagcca ccgcgctcaa aacgctcatc gtcagcagca
     2821 acccgctggc cggcctccag gggctcgagg cgctgcccca gctggatacg ctgaccgccg
     2881 agaccgccgg gctcaaggcg ctggtgaccg tcgccccgaa cacgctgatc ggcctccacc
     2941 tcaacggcaa ccagatcaat gacgtgaccc cgctcgccgg aaatcagttc ctcggcttcg
     3001 tccatctcga cgacaacgcg atcaccaccc tcgcgccgat ccagcaggcc ccgtgggtcc
     3061 agagccactg cgcgaacctc caggtcagcg gcaacccgct cgacgccgcg accctggcga
     3121 ccacgctgcc cgccatgtgc gacctcgaca tgacgatcgc gtggacccaa ggcgagtgcg
     3181 cgccggacgg ctttcgctgc ggcttctcgg acccgtgaac aaacgcccgc catgtgcgag
     3241 ctcgacatga aggtcgcgtg ggacgagggc ccgtgcgggc ccgagggcat gcgctgcggc
     3301 atgggcgggc cctgagcccg cggagccgcg gcgctcagcc gtgctcggcc atccagcgct
     3361 cggcttcgat cgccgccatg cacccggtgc cggccgcggt gatcgcctgg cgatacacct
     3421 tgtccgcgac gtccccgcag gcatacacgc cgggcacccg cgtcgccgcg gtcccgggga
     3481 tcgtcttgag gtagccgagc tcgtccatgt cgagctggcc cttgaagatc gggctgttgg
     3541 ggttgtggcc gatcgccagg aacacgccgg tgactgcgag gtcctcctgc gtgccgttga
     3601 cggtgtcctg caggcgcacg ccgacgacgc ggtcggtgcc gagcacgtcg acgacctcgc
     3661 ggttccagtg cacggtgatc ttcgggttct cgaacacccg cttttgcatg atcttgctcg
     3721 cgcggaagct gtcgcggcgg tggatcatgt gcacgctgcg gcacatgcgg gtcaaaaacg
     3781 tcgcctcctc catcgccgtg tcgccgccgc cgatcacgac gacatcctgg tcgcgataaa
     3841 acgcgccgtc gcaggtggcg caggctgtca cgccgccgcc gctgaggcgc aggcgctgct
     3901 cgttcggcag cccgagccac ttggccgacg cgccggtggc gatgatcagc gtgtcgccgg
     3961 tgtacaccgc cgactcgccg acgatcgtga acggacgctt gctcaggtcg gcctcgagac
     4021 cgaggtcgta ataaagcttg gtgccgaagc gctcggcctg ctcgcgaaac tcgtccatca
     4081 tctccgggcc ggtgatgccc ttggggaagc cagggtagtt ctcgacctcg gtggtcgtcg
     4141 tcagctggcc gccgggctcg ggcccgcaga ccatgatcgg ctcgaggttg gcccgggcgg
     4201 cgtagatcgc cgcggtataa ccggcagggc cggcgccaag gatgagaacc ttgctgtgga
     4261 tcgtctcgcg catgcgcgcc ggaggatagc agcgcccacc cccgccacaa gctgtcacaa
     4321 atcgtctctg gcgcatcccc tgcgcccggg gggcgccatc ccgcgccgaa acctgtcccg
     4381 agagacatgg gacggcgacg atccgccccg gcgagagcgc tgtacccgca tctgcgggga
     4441 ggagagtccg cagggcgagg tgttagagtc gcggcgggcc catcaggtgg ccggaggggg
     4501 gacggctggt tgacaccgag acattgctcg cagacctgcg acggatcgtc agcgagctcg
     4561 cggtgggtcg cggcgtcgag tcggccccgc gggagttggc cgacaaagtc gtcgagctga
     4621 caagcgctct ggatcacctc tacgaccggc tcgacaagat cttgcaggtc gtcac
//
LOCUS       DASWNJ010001612         7690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_450558, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001612 DASWNJ010000000
VERSION     DASWNJ010001612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7690
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450558"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /locus_tag="VGB85_26765"
     CDS             <1..1266
                     /locus_tag="VGB85_26765"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0137728.1"
                     /translation="HAGLQTAAVNLADEVLAAAEKISDRKLLAEALLQRGRLAINRLD
                     HANGQDAVLTRAYLTALGGRLDELATEALALRMYHRSRENGRAAQALDDLDVAREMVE
                     RLPSPERVHGLVLNNAGAVYLASGDIPQATALFREALTAREAALGPEHLEVAYTLANL
                     AMVSAIEDRTPLIERALRIFDRQLGQAHPQTLELRLATSYFTLDPRDARALITPGCEA
                     LGRFASDRTQHARCMLFLGHHAREAGDSKAAAESFLQLDRLFSLGGDAKMEMDPIEIA
                     EIRGYAALYTGKHAEAVELLRKQLAVEGADAEVWQRRHRAELEFLLGLHLDRLGQREE
                     ARRALTSTVATFESAATASPDVLLQQRLAGARSALAAHLLAAGATSEERENATKLLSD
                     AEQWYRGAGAGYAWRLDELAALRRKAALR"
     gene            1401..2396
                     /locus_tag="VGB85_26770"
     CDS             1401..2396
                     /locus_tag="VGB85_26770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADYC domain-containing protein"
                     /protein_id="HEY0137729.1"
                     /translation="MKLLNLLLVNVCTVSIMGLALTGCDPVEDSEGSDQVEHRNGWGY
                     TCPTWQCGFNSAEVNGRAIRELNVNGVANSAGMKIVGFTGPAGLLGYSLAVENDELVA
                     KKTGAPTLRGAQLIGSIILLKEPGLLGLSIPITIAASSEIDSWATGAPDVPAYAFLYP
                     DLLSLTGTRNVCNNGLDELLVSSAVVLGGETYNLTNKTVNANMNGWFTIACAGSAAAK
                     MRLLNYGPQSNFDGTGQPATVAQRQATLKMLTADYCGNGTSYTANGTPLQYEDVEGTV
                     TFSGTQGAPEAIWTAQGALCLDTTRIANTSVACNLPSCSSFDITDGVWATYVPTP"
     gene            2788..3957
                     /locus_tag="VGB85_26775"
     CDS             2788..3957
                     /locus_tag="VGB85_26775"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEY0137730.1"
                     /translation="MRALCWHGKGDVRVDTVPDPKIEDPRDVIVKITSTAICGSDLHL
                     YDGYMPTMESGDILGHEPMGIVAEVGSAVTRLKVGDRVVVPFVIACGACFFCQRTEFS
                     LCDTTNRNAEIARKAMGHSPAGLFGFSHMLGGFAGGQAEYLRVPHADVGPLKIESSLP
                     DEQVLFLSDIFPTGYMAAENCAIEAGDTVAVWGCGPVGQFAIRSAWLLGAARVIAIDR
                     VPERLRMAEAGKAETINFEDTDVYDRLQEMTHGRGPDRCIDAVGCEAHGIGALDATMD
                     KVKAALMLTTDRAHALREAIMCCRKGGTVSVPGAYVGLIDKLPFGAAMNKGLTFKMGQ
                     THMQRYLQPLLQLIERGAIDPSFVITHRLPLEEAPAAYETFRDKKDGCIKVVLKP"
     gene            4116..5078
                     /locus_tag="VGB85_26780"
     CDS             4116..5078
                     /locus_tag="VGB85_26780"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEY0137731.1"
                     /translation="MPSGDVLPVLLSLFTLVAVGQLLRLLAALPANAPEVLARVIVQV
                     TMPALIVVILADARFEPALLPALVATTSALLVALLLGVLLLRTLGASRPAQGAAGIVA
                     SFSNTAFLGIPFITAVLPGSRPAATTAVIIDTVDTTLLLLTLGVAFAGAMARPRPPTS
                     PPLGPRIAVASLRLLRQPMIIAVLVGLVLALTDLDIPRPLAGPLAGVGQATPVLAFLT
                     IGLGLDLGSLRGQTLALAGIAVIKLAVAPVIAALVLLALDVRGEVAQTAVLQAAMPTA
                     MVSVIIAANAGCDARLATAASVVTTVLALATLPLWVMGLQALGL"
     gene            complement(5208..6263)
                     /locus_tag="VGB85_26785"
     CDS             complement(5208..6263)
                     /locus_tag="VGB85_26785"
                     /inference="COORDINATES: protein motif:HMM:NF024209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEY0137732.1"
                     /translation="MKSLNASVVAVFACVCFSGCAEEHTDYFTTGTEGSTGTDASGSS
                     GESTGSDESTSLPPDVTVTTGDDSTTGDDSTTDVPTTTGDDSTTGAPLDCEVVKFADP
                     KVEEKVRAELKLFEGEITGAALLELTSINLFFEPVADLGGLECAHNLEVLSAGYSGVS
                     DLGPVAGLTKLKKIELADNTVTDITPLKGLVQLESLNLQGGDFPDLEPIAGLTKLTHI
                     GLQNTPITDIKVLANMTSLRSLDINYTGVSDISPLTGHPIVVLSARDCELTDVTPIAG
                     FPTPVSIILTGNKITEVDALLTVDWSVADTNMGCADIQLGDNPLSAKALEQVIPALCD
                     KTGLGFMADGVKCGTSC"
     gene            complement(6625..7413)
                     /locus_tag="VGB85_26790"
     CDS             complement(6625..7413)
                     /locus_tag="VGB85_26790"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0137733.1"
                     /translation="MNTNRTQIYRTVVRGKPVRYQVHARADGGATPVLLVHGLGCSSR
                     AFRPTLDLLTERGEPRRAFAPDMPGYGSTPGPRRAMDMEALGAWQLDFLDAIGIPRAH
                     VIGNSMGCQVAMAMARQAPGRVASLILTGPTTGDQYQPLWRYVIGLLADSLFESWSYN
                     ISLARTYLQMGVVRHVETMRHLLRDHPIARASEVVCPTLIVRGKRDLIIPKAIAQRLA
                     AALPRGEYAEVEGVAHAVQHDLPTALWALAEPFFARSTTDASAP"
     gene            complement(7410..>7690)
                     /locus_tag="VGB85_26795"
     CDS             complement(7410..>7690)
                     /locus_tag="VGB85_26795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137734.1"
                     /translation="QDADRLDAIGAVGVARLFATCTAMRTPFYSEDDPMCRVREPEDR
                     RFGLDHFFKKLLKLPGGLHTGTARALAEPRARLMLDYLAQFEQELEGR"
BASE COUNT         1191 a         2781 c         2508 g         1210 t
ORIGIN      
        1 cacgccggct tgcagactgc ggccgtcaac ctcgccgacg aggtcctcgc cgccgccgag
       61 aagatcagcg accgcaagct gctcgccgag gccttgctgc aacgcggccg cctggcgatc
      121 aaccgcctcg atcacgccaa cggccaggac gccgtgctca cccgcgccta tctgacagcg
      181 ctcggcggcc gcctcgacga gctcgcgacc gaggccctcg ccctgcgcat gtatcatcgc
      241 agtcgcgaga acggccgcgc cgcccaggcg ctcgacgacc tcgacgtcgc gcgcgagatg
      301 gtcgagcgcc tgccctcgcc cgagcgcgtc cacggcctcg tgctcaacaa cgccggcgcc
      361 gtgtacctcg ccagcggcga catcccgcag gcgaccgcgc tgttccgcga ggcgctgacg
      421 gcccgggagg ccgcgctcgg tccggagcac ctcgaggtcg cctacaccct ggccaacctc
      481 gcgatggtca gcgcgatcga ggaccgcacc ccgctgatcg agcgcgccct gcggatcttc
      541 gatcgccagc tcggccaggc gcacccgcag accctcgagc tgcggctcgc caccagctac
      601 ttcacgctcg acccccgcga cgcgcgggcg cttatcactc ccggctgcga ggcgctcggt
      661 cgcttcgcca gcgaccgcac ccagcacgcg cgctgcatgc tgttcctcgg tcaccacgcg
      721 cgcgaggccg gcgacagcaa ggccgcggcc gagtccttcc tccagctcga ccggctgttc
      781 tcgctcggcg gcgacgccaa gatggagatg gacccgatcg agatcgccga gatccgcgga
      841 tatgcggcgc tctataccgg caagcacgcc gaggccgtcg agctgctgcg caagcagctc
      901 gcggtcgagg gcgccgacgc ggaggtctgg caacgtcgcc atcgcgccga gctcgagttc
      961 ctgctcggcc tgcacctcga tcgcctcggc cagcgtgagg aggcccgtag agcattgacc
     1021 tctacggtcg ccaccttcga gtccgccgcg accgcgtctc ccgacgtatt gctacaacaa
     1081 cgcctcgcgg gcgcgcgttc ggccctggca gcccatctgc tggcagcagg cgccacttcc
     1141 gaggagcgag agaacgcgac aaaactgctc tcggatgcgg aacagtggta tcgcggagca
     1201 ggcgccggct atgcctggcg tctcgatgaa ttggcggcat tgcgacgcaa ggcagctttg
     1261 cgatgattgc cgcacgtacg cacatacata catgaccgcc caaatctcgt cacaaacctc
     1321 cgaaagatcc ttgcagtgtt gtttttttca tacacataat agccatcaac cgcgccgctg
     1381 aagcgcaaag gtggtgtccg atgaaactgc tcaacctgct ccttgtgaat gtgtgtactg
     1441 tcagcatcat gggtctcgcg ctgaccggct gcgaccccgt cgaggactcc gaggggtcgg
     1501 atcaggtcga gcatcgtaat ggctggggct acacctgtcc cacctggcaa tgcgggttca
     1561 actccgcgga ggtcaatggt cgtgcgatcc gcgagctcaa cgtcaatggg gtcgccaact
     1621 cggccggcat gaagatcgtc ggcttcaccg gcccggccgg cctgctcggc tactctctcg
     1681 ccgtcgagaa cgacgagctg gtcgccaaga agaccggcgc gccgaccctg cgcggcgcgc
     1741 agctgatcgg cagcatcatc ctgctcaagg agcccggcct gctcggcctg agcatcccga
     1801 tcacgatcgc cgcctccagc gagatcgact cctgggccac cggcgccccg gacgtgccgg
     1861 cttacgcgtt cctctacccg gacctgctct cgctgaccgg cacgcgcaac gtctgcaaca
     1921 acggcctcga cgagctcctc gttagctccg cggtcgtgct cggcggcgag acctacaacc
     1981 tgaccaacaa gacggtcaac gcgaacatga acggctggtt cacgatcgcc tgcgccggct
     2041 cggccgcggc gaagatgcgc ctcctcaact acggcccgca gtccaacttc gacggcaccg
     2101 gccagcccgc caccgtcgcc cagcgccagg ccaccctcaa gatgctcacc gccgactact
     2161 gcggcaacgg caccagctac accgccaacg gcacccccct gcagtacgag gacgtcgaag
     2221 gcactgtcac tttcagcggc acccagggcg cccccgaggc catctggacc gcccagggcg
     2281 ccctgtgcct cgataccacc cgcatcgcca acaccagcgt cgcttgcaac ctcccgagct
     2341 gctcgagctt cgacatcacg gacggcgtgt gggccaccta cgtcccgacc ccctgaagtc
     2401 tgctcgcgcc ggttcgagct gaaatgatgt gctcgcggat gactcccgga ccatcggcgg
     2461 tccaggaaga gacaccatga gcaaacaaga atgatgggag cgagggcagc ggcgatacgc
     2521 gctgaccgac gatagagaac ggcccgcggc gctcgctgcg ggccgtgcct ttttcggggc
     2581 attaccagta aaaaccctga actttgcggc ccgcgtccac tgcgcataat cccgccaaag
     2641 atccgcgatt tgccggtcca gggccgcctg cgggctggcg gacagcgctc gtacggatgt
     2701 ggccggacgg cagggatggc ccaggcttgg ctaccttcgt gccgccatcc ggtggccacg
     2761 agcaacccct gtccggagaa gatccccatg agagcgcttt gctggcacgg caagggcgat
     2821 gtccgcgtcg acaccgtccc cgaccccaag atcgaagacc cgcgcgacgt gatcgtcaag
     2881 atcacgtcga ctgcgatctg cggctcggac ctccacctct acgacggcta catgccgacc
     2941 atggagtctg gtgacatcct cggccacgag ccgatgggca tcgtcgccga ggtcggcagc
     3001 gcggtgacgc ggctcaaggt cggtgaccgc gtcgtcgtcc ccttcgtcat cgcctgcggc
     3061 gcgtgcttct tctgccagcg caccgagttc tccctgtgcg acacgaccaa ccgcaacgcc
     3121 gagatcgccc gcaaggcgat gggccactcg cccgccggcc tgttcggctt ctcgcacatg
     3181 ctcggcggct tcgctggcgg ccaggccgag tacctgcggg tgccccacgc cgacgtcggc
     3241 ccgctcaaga tcgagtcgag cctccccgac gagcaggtcc tgttcctctc cgacatcttc
     3301 ccgaccggct acatggcggc cgagaactgc gcgatcgaag ccggtgatac cgtcgccgtg
     3361 tggggctgcg gccccgtcgg ccagttcgcc atccgcagcg cctggctgct cggcgcagcc
     3421 cgcgtgatcg cgatcgaccg cgtgcccgag cgcctgcgca tggccgaggc cggcaaggcc
     3481 gagacgatca acttcgagga caccgacgtc tacgaccgcc tgcaagagat gacccacggc
     3541 cgcggccccg atcgttgcat cgacgccgtc ggctgcgagg cccacggcat cggcgccctc
     3601 gatgcgacga tggacaaggt caaggcggcc ctgatgctga ccaccgaccg cgcgcacgcc
     3661 ctgcgcgagg cgatcatgtg ctgccgcaag ggcggcaccg tgtcggtgcc cggcgcctac
     3721 gtcggcctga tcgacaagct gccctttggc gccgcgatga acaagggcct gactttcaag
     3781 atgggtcaga cccacatgca gcgctacctc cagccgctgc tgcagctgat cgagcgcggc
     3841 gcgatcgacc cgtcgttcgt catcacccac cgcctgccgc tcgaggaggc gcccgccgcc
     3901 tacgagacct tccgcgacaa gaaggacggc tgcatcaagg tcgtgctcaa gccctgaccc
     3961 gatcggggcc cgcacacgga cagagcgcct cgccggtccc ccggcgaggc gctctgtaat
     4021 caccacgata ccggcgcacc atggctgatt catcggccat ccgcgacgcc gacacgacca
     4081 tcgacggcct tcgagatccg cggtagacca ccgcggtgcc ctcgggcgac gtcctcccgg
     4141 tcctgctgtc cctgttcacg ctcgtcgccg tcggccagct gttgcgcctg ctcgcggcgc
     4201 tccccgcgaa cgcgcccgag gtcctcgctc gcgtcatcgt ccaggtgacg atgccggccc
     4261 tcatcgtcgt catcctcgcc gacgcccgct tcgaacccgc cctgctgccg gccctcgtcg
     4321 cgacgacctc cgccctgctg gtcgcgctgc tgctcggggt gctgctgctg cgcacactcg
     4381 gcgcctcgcg cccggcccag ggcgccgccg gcatcgtcgc gagtttctcg aatactgcct
     4441 tcctcggcat ccccttcatc accgcggtgc tgcccggctc gcgcccggcg gcgacgactg
     4501 ccgtcatcat cgacaccgtc gacacgacgc tgctgctgct caccctcggg gtcgcgttcg
     4561 ccggcgcgat ggcccgaccg cggccgccga cgagcccgcc gctgggacca cgcatcgcgg
     4621 tggcgtcact tcgcctgctg cgccagccga tgatcatcgc ggtgctcgtc ggcctcgtgc
     4681 tcgcgctcac cgacctcgac atcccccgcc cgctcgccgg cccgctcgcc ggggtcggtc
     4741 aggccacccc cgtgctcgcc ttcctgacga tcggcctcgg cctcgacctc ggctccctgc
     4801 gcggccagac cctcgcgctc gccggcatcg ccgtcatcaa actcgcggtc gcacccgtca
     4861 tcgccgcgct ggtcctgctc gccctcgacg tccgcggcga ggtcgcccag accgccgtgc
     4921 tgcaggccgc gatgccgacg gcgatggtct ccgtcatcat cgccgccaac gccggctgcg
     4981 acgccaggct ggcgaccgcg gcctccgtgg tcaccacagt gctcgcgctg gccaccctgc
     5041 cgctgtgggt catgggcctg caggcgctcg gcctgtgaag ctcaccggcg cgtcaaaacc
     5101 gcgagggtct caagatcaag ggcgtcacct gctgcaggtt cgtgtgagct ggcggccgcc
     5161 gctcgcccgg gttcaacatt gaacggcccg ggcgccccgg cggcgcgcta gcaggatgtg
     5221 ccgcacttga cgccgtcggc catgaagccc agcccggtct tgtcgcacag ggccgggatg
     5281 acctgctcga gcgccttggc cgacagcgga ttgtcaccga gctggatgtc ggcgcagccc
     5341 atgttcgtgt cggcgaccga ccagtcgacg gtgagcagcg cgtcgacctc ggtgatcttg
     5401 ttgcccgtga ggatgatcga tacgggggtc gggaagccgg cgatgggcgt cacgtcggtg
     5461 agctcgcagt ctctcgcgga gagcaccacg atcgggtgac cggtcagcgg ggagatgtcc
     5521 gagaccccgg tgtagttgat gtcgagcgag cgcagcgagg tcatgttcgc cagcactttg
     5581 atgtccgtga tcggggtgtt ttgcaggccg atgtgggtca gcttggtgag gccggcgatg
     5641 ggctcgaggt cggggaagtc gccgccctgc agattgagcg actcgagctg cacgaggccc
     5701 ttgagcgggg tgatatccgt gaccgtgttg tcggccagct cgatcttctt gagcttggtg
     5761 aggccggcca ccgggccgag gtcggagacc ccggagtagc cagcgctgag gacctcgagg
     5821 ttgtgcgcgc actcgaggcc gccgaggtcg gccaccggtt cgaagaacag gttgatgctc
     5881 gtgagctcga gcagcgccgc gccggtgatc tcgccctcga agagcttgag ctccgcgcgg
     5941 accttctcct cgacctttgg atcggcgaac ttgaccacct cgcagtcgag cggcgcgccg
     6001 gtggtgctgt catcgccggt ggtggtgggg acgtcggtgg tgctgtcatc gccggtggtg
     6061 ctgtcatcgc cggtggtgac ggtcacgtcg ggaggcaaac tggtcgactc gtccgagccg
     6121 gtcgactcgc cgctggagcc gcttgcgtcg gtgccggtcg acccctcggt gccggtcgtg
     6181 aagtagtccg tgtgttcctc ggcgcacccc gagaagcaca cacaggcgaa gacggcgacg
     6241 accgatgcgt tgagcgattt catgagtggt cctggtccgg gagcaggacc ggcaccctgc
     6301 agagaatgtg ccacccctgt tcgcgcgcag acgcggcgcc aggggcctgc attgtcgcct
     6361 gcgcggtcaa tgacgccgtg gttgtggcct gccagtcgac atgcgcggcc gcggacgcct
     6421 tcagtcgacg aagtgggtcc gcatgcgggt gacgtggaag ccggagtatg ccggacgggc
     6481 accgccgggc gctcgagcac acggccggta tcatcgtggc cgcgacgaag acgacccgcg
     6541 cgcgaccccc gaagccgttc gagccgctgc gagacgtccc gtgggcgccg gggcgtggtc
     6601 ggctgtcaag cgctgcgcgt ggtgtcaggg tgcgctcgcg tccgtggtgc tgcgggcgaa
     6661 aaagggctct gcgagggccc acagggcagt gggtaggtca tgctggaccg cgtgcgcgac
     6721 gccctcgacc tccgcgtact cgccgcgcgg caacgcggcg gcgagccgct gcgcgatcgc
     6781 ctttgggatg atgaggtccc gcttgccgcg gacgatcagc gtcgggcaca cgacctcgct
     6841 cgcgcgcgcg atcgggtggt cgcgcagcag gtgtcgcatc gtctcgacgt ggcggacgac
     6901 gcccatctgt aaataagtgc gggccagcga gatgttgtac gaccacgact cgaacagcga
     6961 gtcggcgagc agcccgatca cgtagcgcca cagtggttga tactggtcgc cggtggtcgg
     7021 cccggtgagg atcaacgagg cgacgcggcc cggcgcctgg cgggccatcg ccatcgccac
     7081 ctgacagccc atcgagttcc cgatcacatg tgcgcgcggg atcccgatcg cgtcgaggaa
     7141 gtcgagctgc caggcgccga gcgcctccat gtccatcgcc cggcgcgggc ccggcgtgct
     7201 gccgtagccg ggcatgtccg gcgcgaacgc gcgacgcggc tcgccacgct ccgtcaacag
     7261 gtcgagcgtc gggcgaaacg cccggctcga gcagccgagg ccgtgcacca gcaacaccgg
     7321 cgtggcccca ccgtcggccc gcgcgtgcac ctgatagcgc accggcttgc cacgaacgac
     7381 ggtccggtag atctgcgtac ggttggtgtt catcggcctt ccagctcctg ttcgaattgc
     7441 gcgaggtagt cgagcatcag ccgtgcgcgc ggctcggcga gcgcgcgcgc ggtgcccgtg
     7501 tgcagcccac ccggcaactt gagcagcttc ttgaagaagt gatcgagccc gaagcgtcgg
     7561 tcctctggct cgcgcacgcg acacatcgga tcgtcctcgc tgtaaaacgg cgtgcgcatc
     7621 gccgtgcagg tcgcgaacaa tcgggcgacg ccgaccgcac cgatcgcgtc gagccggtcg
     7681 gcgtcctgca
//
LOCUS       DASWNJ010001613         1608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_522139, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001613 DASWNJ010000000
VERSION     DASWNJ010001613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522139"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VGB85_26800"
     CDS             <1..890
                     /locus_tag="VGB85_26800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137735.1"
                     /translation="PELAARTRFEPSAIAWSASRERFVIASDDTGLPERDEHAPWLFT
                     MDPRGHVDPEPLVVAGLGAFSDIEALAAGPGDSMYMLASQSFSKKGKRSAARQVFARV
                     IVDRAGARAEASVKLAESIDKGGADLLATLGLPDTRGLDLEGLTASASGGLLIGLKEP
                     LDAGEQAVIWQLARPGMLLAGGDVTGAGLTRFGAAPVKIVADGASRAGGISELLELPD
                     GSLLIASTASGTEPASQDGALWHARGRDGLASPRMLRTFPGLKPEGLALRPDGAAIVI
                     VFDTGAAPPQWMELPWPAP"
     gene            875..>1608
                     /locus_tag="VGB85_26805"
     CDS             875..>1608
                     /locus_tag="VGB85_26805"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY0137736.1"
                     /translation="MARSVIAALLLGGCFHAPAYSPQRSVATWREMQSAAPAGATSSR
                     ADAGGDALTADEAYAYALRNNPELAVAEAEAEVIAAEVDAAKQIDNPQLRLTNFGVDD
                     AVRGAPGLNLALRAPIPRPGTVRARAEGARLAAEAAQGQTEAARRLLRAEIDRLYARL
                     AMLRADLEEVTRAASLGDERHKQIRERVERQVGTRVDLALADLARAEALDEASQLRDE
                     LATTEAELARVIGPGAPRKYHVDPNVL"
BASE COUNT          212 a          605 c          603 g          188 t
ORIGIN      
        1 cgccggagct ggccgctcgc acgcgcttcg agccctcggc gatcgcctgg tcggcgagcc
       61 gcgagcgctt cgtgatcgcc agcgacgaca ccggcctgcc cgagcgtgac gagcacgcgc
      121 cgtggctgtt cacgatggac ccccgcggcc acgtcgaccc cgagccgctc gtcgtcgccg
      181 gcctcggggc ttttagcgac atcgaggcgc tcgcggccgg gcccggcgac agcatgtaca
      241 tgctcgcgtc gcagagtttc agcaagaagg gcaagcgcag cgcggcgcgt caggtgttcg
      301 cgcgggtgat cgtcgaccgc gccggggcgc gcgcggaggc ctcggtcaag ctcgccgaga
      361 gtattgataa gggcggcgcg gacctgctgg cgacgctcgg cctgcccgac acccgcggcc
      421 tcgacctcga agggctgacc gcgagcgcga gcggcggact gttgatcggg ctcaaggagc
      481 cgctcgacgc cggtgagcag gcggtgatct ggcagctcgc gcggccgggc atgctgctcg
      541 ccggcggtga tgtcacgggc gccggcctga cgcgcttcgg cgccgcgccg gtgaagatcg
      601 tcgccgacgg cgcgagccgt gcaggtggga tctccgagct gctcgagctc ccggacggct
      661 cgctgctgat cgccagcacc gcctcgggca cggagcccgc gagccaggac ggcgcgctgt
      721 ggcacgcacg cggccgcgac ggcctggcga gcccacggat gctgcgcacg ttccccggcc
      781 tcaagcccga gggcctggcg ctgcgcccgg acggcgccgc gatcgtgatc gtgttcgaca
      841 ccggcgccgc gccgccgcag tggatggagc tgccgtggcc cgctccgtga tcgcggcgct
      901 gctgctcggc ggatgcttcc acgcgcccgc ctattccccg cagcggtcgg tcgcgacctg
      961 gcgcgagatg cagtccgccg cgcccgcggg cgcgacgagc tcgcgggcgg acgccggcgg
     1021 tgatgcattg acggccgacg aggcgtacgc ctacgcgctg cgcaacaacc ccgagctcgc
     1081 ggtcgccgag gccgaggccg aggtgatcgc ggccgaggtc gacgccgcca aacagatcga
     1141 caacccgcag ctgcgcctga ccaacttcgg ggtcgacgac gcggtgcgcg gcgctcccgg
     1201 cctcaacctc gccctgcgcg caccgatccc gcggcccggc acggtgcgcg cgcgggccga
     1261 gggcgcacgc ctcgcggccg aggccgccca gggccagacg gaggccgcca ggcgcctgct
     1321 gcgggccgag atcgatcgcc tctacgcgcg cctggcgatg ctgcgcgccg acctcgagga
     1381 ggtcacgcgc gcggcgagcc tcggcgacga gcgccacaag cagatccgcg agcgcgtcga
     1441 gcgacaggtc ggcacccgcg tcgacctcgc gctcgccgac ctcgcgcgcg cggaggcgct
     1501 cgacgaggcg agccagctcc gcgacgagct ggcgaccacc gaggccgagc tcgcgcgggt
     1561 gatcggcccg ggcgcgccgc gcaagtatca cgtcgacccc aacgtgct
//
LOCUS       DASWNJ010001614         3169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_380213, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001614 DASWNJ010000000
VERSION     DASWNJ010001614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380213"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VGB85_26810"
     CDS             complement(<1..313)
                     /locus_tag="VGB85_26810"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEY0137737.1"
                     /translation="MPLTPEQEWIIAACGLIAHADGDLSPGESDQVLAMLDDSLSPQE
                     RARWLNLLTDGAALARTFRELAPPLPAFVEPLLEQAWSMALADGHASESEVRELERIA
                     GD"
     gene            complement(398..1723)
                     /locus_tag="VGB85_26815"
     CDS             complement(398..1723)
                     /locus_tag="VGB85_26815"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0137738.1"
                     /translation="MGLLLSACSDDGVDPSTVTGLPVTTESDGSSGSSGDATSTPTTG
                     DTEAPSTGEPMPSCGDGIKDPGELCDDGDVDNTDYCLGTCMLATCGDAFVQAGIEQCD
                     DGNNSDEDSCVAGCYLPTCGDGHTYAGVEGCDDGNKQDGDGCDAECEIETQMCGDGIV
                     EGDEACDDANADDSDDCLANCVAATCGDGYLHAALETCDDGNPDDTDDCTSLCQIAIC
                     GDGLVHKGDEECDDANDIDTDACLTGCKNAVCGDAVVQAGVEVCDDGKNIGEYGGCGK
                     GCAALAPRCGDAVLDEGVETCDDANLVDGDGCSSACQLELPPECLGYVELKEPDRAAS
                     FNDGPGKVTKCDKTAEGKWHRFLDPAGVVMPLVAPSQYSCGTDSPGYMLGTYPFEDEG
                     IVPRTVCFPWFNDPCMWSHEIAVRNCGEYFVFQLPTPSTCALRYCAAPL"
     gene            complement(2070..2288)
                     /gene="infA"
                     /locus_tag="VGB85_26820"
     CDS             complement(2070..2288)
                     /gene="infA"
                     /locus_tag="VGB85_26820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY0137739.1"
                     /translation="MSKDDLIEFEGTVTEVLTGGLFRVEIDKDHTVLAHLAGKMRRFR
                     IKVVLGDRVTVGVTPYDPTRGRITYRAR"
     gene            complement(2441..3055)
                     /gene="cysC"
                     /locus_tag="VGB85_26825"
     CDS             complement(2441..3055)
                     /gene="cysC"
                     /locus_tag="VGB85_26825"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEY0137740.1"
                     /translation="MNHAPNIRWHHATVTAEDRERSKGQRGAALWFTGLSGAGKSTVA
                     AIVDHRLQQAGRHSFILDGDNLRHGLCKDLGFAPEDRAENIRRLGEVARLFVEAGLIV
                     LCAAISPYRRDRDQARALVGDGRFIEIFVDTDLAECERRDPKGLYRKARRGEITGFSG
                     IDAPYERPEAPELRLVAGTAGPEALADEVLAHLQARGIVGLTPP"
BASE COUNT          422 a         1099 c         1156 g          492 t
ORIGIN      
        1 ggtcgcccgc gatccgctcg agctcgcgga cctcggactc gctcgcgtgc ccgtccgcga
       61 gggccatcga ccacgcctgc tcgagcagcg gctcgacgaa cgccggcagc ggcggcgcca
      121 gctcgcggaa cgtgcgcgcg agggcggcgc cgtcggtcaa caggttgagc cagcgcgcgc
      181 gctcctgcgg gctcaggctg tcgtcgagca tcgccagcac ctggtcgctc tcacccggcg
      241 agaggtcgcc gtcggcgtgg gcgatgaggc cgcaggcggc gatgatccat tcctgttccg
      301 gcgtcagtgg catcgagcct ccacggccga gggtacaccg atcgcgcgct ggcttgcgcg
      361 cgaatgcgcc ctcgtgcgag cgtccgccgg gaccgctcta aagcggcgcc gcgcagtaac
      421 gcaaggcgca ggtcgagggc gtcggcagct ggaacacgaa gtactcgccg cagttacgca
      481 cggcgatctc gtgggaccac atgcaggggt cgttgaacca ggggaagcag accgtgcgcg
      541 ggacgatgcc ctcgtcctcg aacgggtagg tgccgagcat gtagccgggc gagtcggtgc
      601 cgcacgagta ctggctcggg gcgacgagcg gcatgacgac gccggccggg tcgaggaagc
      661 ggtgccactt gccctcggcc gtcttgtcgc acttggtgac cttgccgggg ccgtcgttga
      721 agctcgcggc gcggtcgggc tccttgagct cgacgtagcc gaggcactcg ggcggcagct
      781 cgagctgaca ggcgctgctg cagccgtcgc cgtcgaccag gttggcgtcg tcgcaggtct
      841 cgacgccctc gtcgagcacc gcgtcgccgc agcgcggggc gagggccgcg cagcccttgc
      901 cgcagccgcc gtactcgccg atgttcttgc cgtcgtcaca cacctcgacg cccgcttgca
      961 cgaccgcgtc gccgcagacg gcgttcttgc agccggtgag gcacgcgtcg gtgtcgatgt
     1021 cgttggcgtc gtcacactcc tcgtcgccct tgtgcaccag cccatcgccg catatggcga
     1081 tctggcacag gctggtgcag tcgtcggtgt cgtcggggtt gccgtcgtcg caggtctcga
     1141 gggccgcgtg gaggtagccg tcgccgcaag tggccgcgac gcagttggcg aggcagtcgt
     1201 cgctgtcgtc ggcgttggcg tcgtcgcagg cctcgtcgcc ctcgacgatg ccgtcgccgc
     1261 acatctgcgt ctcgatctcg cactcggcgt cgcagccgtc gccgtcctgc ttgttgccgt
     1321 cgtcgcagcc ctcgacgccg gcgtaggtgt ggccgtcgcc gcaggtgggg aggtaacagc
     1381 cggcgacgca gctgtcctcg tcgctgttgt tgccgtcgtc acactgctcg atgccggcct
     1441 gcacgaacgc gtcgccgcag gtcgcgagca tgcaggtgcc gaggcagtag tcggtgttgt
     1501 cgacgtcgcc gtcgtcgcac agctcgccgg ggtccttgat gccgtcgccg cagctcggca
     1561 tcggctcgcc ggtggacggc gcctcggtgt cgccggtggt cggcgtgctg gtcgcgtcgc
     1621 cgctgctgcc gctgctaccg tcgctctcgg tcgtcaccgg cagaccggtg acggtgctcg
     1681 ggtcgacgcc gtcgtcggag cacgccgaca gcagcagccc cacgcccaag gcccatccaa
     1741 acaccccgct cgcgccgctc agcgacccgc accgcatact cgcttgctac caccgatcgc
     1801 gccgagatcg caacgacgcg cgcatacggc cgcagatact gcacggcgcc gtcgtcaccg
     1861 cttccaccct cgcgtcggcc tcgcgtcggc ctcgcgtcgg catgcgctcg ccgcttcaca
     1921 cgacgcgtgg gtcacacgcg agcagaaacg acgacgcgcg gcgcacgggg gaccgtggcc
     1981 acgcgaggca ggtacagcga gtcaggagtc ggcgcggctg gcccggccga ttagcagagg
     2041 cgagcagcag aggcggctgg cccggccagc tagcgggcgc ggtaggtgat gcgaccgcgg
     2101 gtggggtcgt agggggtgac gccgacggtg acgcggtcac cgagcaccac cttgatgcga
     2161 aagcgccgca tcttgccggc gaggtgcgcg aggacggtgt ggtccttatc gatctcgacg
     2221 cggaagaggc ctcctgtgag cacctccgtc acggttccct caaattcaat cagatcgtcc
     2281 ttgctcatcg gcgacggact gtaggggtgg gcctgcgcga gcgcaacggg ggcggcgaca
     2341 tttcgacaag cgcccgcgca cggcggcagg gctcgttcgg ggcctgcgcg agttaagtgc
     2401 gtttgcgcgc ggatcgcctc acgcggcggt gcgagccgcc tcagggcggc gtgaggccca
     2461 cgatcccgcg ggcttgcagg tgcgcgagga cctcgtcggc gagcgcctcg gggccggccg
     2521 ttccggccac gagccgcagc tcgggggcct cggggcgctc gtagggggcg tcgatcccgg
     2581 agaagccggt gatctcgccg cggcgggcct tgcgatacag gcccttggga tcgcgtcgct
     2641 cgcactcggc gagatcggtg tcgacgaaga tctcgatgaa gcgaccgtca ccgaccagcg
     2701 cgcgcgcctg gtcgcggtcc cggcgatacg gcgagatcgc ggcgcagagc acgatgaggc
     2761 cggcctcgac gaacaggcgc gccacctcgc cgagccggcg gatgttctcc gcgcggtcct
     2821 ccggcgcgaa gccgaggtcc ttgcacaggc catggcgcag gttgtcgccg tcgaggatga
     2881 agctgtggcg accggcctgc tggaggcggt gatcgacgat cgccgcgacc gtgctcttgc
     2941 cggcgccgct cagaccggtg aaccacagcg ctgccccgcg ctggcccttg ctgcgctcgc
     3001 ggtcctcggc ggtgacggtc gcgtggtgcc agcgaatgtt gggggcgtgg ttcacggcgg
     3061 cggcatccta tggcataccc cgcctctgtg cacgaggtcg cctccggtgg cccgaagggt
     3121 tgacggcgat cctcgccgga gtaaccttcg ccggcccgac gcccgtgca
//
LOCUS       DASWNJ010001615         2425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_560574, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001615 DASWNJ010000000
VERSION     DASWNJ010001615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560574"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VGB85_26830"
     CDS             complement(<1..482)
                     /locus_tag="VGB85_26830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137741.1"
                     /translation="MPVGNWLILLVALVIVGVDISVTAPWTGLPVLVVMVIVVLAVVE
                     RMRQLYGERRSPTPAGQPANWLGGVLLFEGVLLAALFGFMVARGLVVWHMFTPEGADS
                     LARAAAAQTYDLVFTVLAGFAGGMVIAPDRTGRVLLGMAQRPAVLLAGSFAAMIAVGS
                     L"
     gene            585..1931
                     /locus_tag="VGB85_26835"
     CDS             585..1931
                     /locus_tag="VGB85_26835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137742.1"
                     /translation="MPTRLAPPASLLPLASLFTLALALAGASGCAASACTKVRNDRQA
                     FLQRRGSGDAAQLVVAVPLATLSQSLTFPLSRIQPIKVPLPDLELPQVPGLGIDLGSL
                     EVGLRSVQVLPAAEGQVGLRVTFALRSRGRDITAIDLDAVIAPQITPGDASVRLSLRP
                     QDLVRVRPSLPPAERKKFADFIMSLVPDGADRLVSRGRVEQLTDKILGDFVGGQFPRV
                     RDQLLGNLDTLVDVEIDLPPVPLARVVLRSSPTDLELWLHSTLPAAPLAPGPARAPGS
                     DPRLVHVRMSGGTAAELANQGIARGQIPARYDEAGEANPNGEFEAGVGWQPGPRPLRL
                     YAWKQTGVCAKAVFAGTPALTAERGDLALAVPDARIEKVTGAFKARVAVWYSGLGKQT
                     FAFSQAVAGATEFELLGVDYDATPLRAAVVGDDLTIDLSLAPTPRPATRRPATRKP"
BASE COUNT          366 a          993 c          737 g          329 t
ORIGIN      
        1 aggctgccga ccgcgatcat cgccgcgaag ctcccggcga gcagcaccgc gggtcgctgg
       61 gccatgccca gcagcacacg tccggtgcgg tccggcgcga tcaccatccc gcccgcgaag
      121 ccggccagca ccgtgaacac caggtcgtag gtctgcgccg ccgcggcccg ggccagcgag
      181 tccgcgccct cgggcgtgaa catgtgccag accaccagcc cgcgcgcgac catgaagccg
      241 aacagcgcgg ccagcagcac cccctcgaac agcaacacgc cgccgagcca gttcgccggc
      301 tggcccgccg gcgtcgggct gcgccgctcg ccgtacagct ggcgcatgcg ctcgacgacc
      361 gcgagcacca cgatcaccat caccacgagc acgggcagcc cggtccacgg cgcggtgacg
      421 ctgatgtcca cgccgacgat caccagagcc acgaggagga tcagccagtt tccgacgggc
      481 acccgcccat tgtacgcgca cgcaccgctg gcacgtcgac ctcgcccgcc ggggccaccc
      541 tcccccaccc cgaccctgga ctcgcggtag actcgcgcca ccgcatgccg acccgcctcg
      601 caccgcccgc ctcgctgctc ccgctcgcct cgctgttcac cctggcgctc gcgctcgcgg
      661 gcgccagcgg ctgcgcggcg tcggcgtgca ccaaggtacg caacgaccga caggcgttct
      721 tgcagcggag gggctccggc gacgccgcgc agctggtcgt cgccgtcccg ctggcgaccc
      781 tgagccagag cttgaccttc ccgctctcgc gcatccagcc gatcaaggtc ccgctgcccg
      841 atctcgagct gccgcaagtg ccgggcctcg ggatcgacct cggctcgctc gaggtcggcc
      901 tgcgctccgt ccaggtcctg cccgccgccg agggccaggt cggcctgcgc gtcaccttcg
      961 cgctgcgcag ccgcggacgg gacatcaccg ccatcgacct cgacgccgtc atcgccccgc
     1021 agatcacccc cggcgacgcc agcgtgcgcc tgtctctgcg cccgcaggac ctcgtgcgcg
     1081 tgcgtccgag cctgccgccc gccgaacgca agaagttcgc cgacttcatc atgagcctcg
     1141 tgccagatgg cgccgaccgc ctggtcagcc gcggccgcgt cgagcagctc accgacaaga
     1201 tcctcggcga cttcgtcggc ggccagttcc ccagggtccg cgaccagctg ctcggcaacc
     1261 tcgacaccct cgtcgacgtc gagatcgacc tgccgcccgt gccgctcgcg cgcgtggtcc
     1321 tgcgctcctc accgaccgac ctcgagctgt ggctgcactc gacgctgccc gccgccccgc
     1381 tcgcccccgg ccccgcgcgc gcccccggca gcgacccgcg cctggtccac gtccgcatgt
     1441 ccggagggac agccgccgag cttgccaacc aaggcatcgc gcgcggccag atccccgccc
     1501 gctacgacga ggcgggcgaa gccaacccca acggcgagtt cgaggccggc gtcggctggc
     1561 aacctggacc gcgcccgctg cgtctgtacg cctggaagca gaccggcgtg tgtgccaagg
     1621 ccgtgttcgc cggcaccccc gcgttgacgg ccgagcgtgg cgacctcgcg ctcgccgtgc
     1681 ccgacgcgcg catcgagaag gtcaccggcg cgttcaaggc ccgcgtcgcc gtgtggtact
     1741 cggggctcgg caagcagacc ttcgccttct cgcaggcggt cgccggggcc accgagttcg
     1801 agctgctcgg cgtcgactac gacgcgaccc cgctgcgcgc cgcggtcgtc ggcgacgacc
     1861 tgacgatcga cctctcgctc gcgccgacgc cccgccccgc gacgcgccgg ccggccacgc
     1921 gcaaaccctg agcgcgtccc cgcccgctgt cctttcggac atctcgtgat cgtatcgccg
     1981 cgatcacgaa cgagagcacc tccagggatc gcctcgtgag cgtgaacaca ggctcacaga
     2041 cgcgcgttca cccattgaac actttgttta gtggcttaca aaaaaatcag tagaggtcgc
     2101 gcttgatcca gcatcgagcc acccatagcg ccacttatcc acagatccca aacggccaat
     2161 tttccccgct gcgggcctcc acatgggaga tacgccggtc gctatccccg cactgcacaa
     2221 gctcgctttt gccgcgccgc gtctcccgcg tcgcgaagcg ttcgacttgc gttcgccgta
     2281 tcccaacccg aagccgctgc ataaactccg ggcatcgacg cgcttccggg aatgacccgg
     2341 ttcgcgtcac tgctgggagc ccgagtgatg accccccccc cctcgacccc gaagaaaccc
     2401 cgtcgcgtca cgccgaagat catcg
//
LOCUS       DASWNJ010001616         2887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_624700, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001616 DASWNJ010000000
VERSION     DASWNJ010001616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624700"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VGB85_26840"
     CDS             complement(<1..231)
                     /locus_tag="VGB85_26840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0137743.1"
                     /translation="MQYRQLGHSGLRVSTLCLGTMTFGEADETSFMHKVGCDEPTAGR
                     IMDRALDAGVNFFDTADVYGNDGLTEQVIGRWF"
     gene            complement(293..490)
                     /locus_tag="VGB85_26845"
     CDS             complement(293..490)
                     /locus_tag="VGB85_26845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137744.1"
                     /translation="MPTKSEVPWVYLCLKVQYAWPQAGARLALGLPHAWPANGRDEPR
                     APVGPGGLALASLESAVGGLP"
     gene            complement(1342..2091)
                     /locus_tag="VGB85_26850"
     CDS             complement(1342..2091)
                     /locus_tag="VGB85_26850"
                     /inference="COORDINATES: protein motif:HMM:NF018917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HEY0137745.1"
                     /translation="MPSSVIGGAALALRGLRLAVDSPEVRRVYLHLALSLVVTSIALM
                     AGLGWAIWALIPTPENPAAWLSALLWTLKIGGTALAMMAAPLLSLFIVNMVFPFLGER
                     VFMAGLKLVDPGRAAELEASGGVAFKTSIASSLRRLLYFIGVTLLTFGLTFVPVLGAV
                     LGPAAQLWFASRMLSWELLDPYFERRGLAYAAQKGLMKKHSTAMFGFGAPWTLLLALP
                     IVGPLGFGLAQAAAALLVTDILETRGRTAAG"
     gene            complement(2103..>2887)
                     /locus_tag="VGB85_26855"
     CDS             complement(2103..>2887)
                     /locus_tag="VGB85_26855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137746.1"
                     /translation="PQALGMPVNVQQGDLDMWLSYTGLILLWSSYRDGAQDTYRASRV
                     EVGMPFEFSYANNKDIGLSTAGEDGKLALSNVDARAYLSTKQPGDPGYRIYTGDRKGQ
                     NYGPLTPLALEFPGATDAFDPHVSSDDLRLYYAPAGGGQRLVVATRPGPDQPFAAPSA
                     EPFVNVDDPERNEADPTLPAHELVLIYTRTGAIDSGGNELWFATRPAIDVPFGPGQPV
                     PGVNGPAHDAGAHLSRDGCELFFARSTVQDEGAWDIYRSEIQ"
BASE COUNT          492 a         1017 c          979 g          399 t
ORIGIN      
        1 gaaccatcga ccgatcacct gctcggtcag gccgtcgttg ccgtacacgt ccgcggtatc
       61 gaagaagttg acgcccgcgt cgagcgcacg gtccatgatg cgaccggcgg tcggctcgtc
      121 gcagccgacc ttgtgcatga aggaggtctc gtcggcctca ccgaaggtca tcgtgccgag
      181 gcagagcgtc gacacccgca ggccgctgtg gccgagctga cggtattgca tgcgcccgac
      241 cctacgcccg gccgcgcgag atctgacggc tcaggagatc aggacatgct tgtcagggca
      301 gaccgccgac cgccgactcg agcgaggcca gcgccagacc gcccggcccg accggcgcgc
      361 gcggctcgtc gcggccgttg gccggccagg cgtgcggcag gccgagagcc agccgggcgc
      421 ccgcctgagg ccaggcgtat tggaccttca gacagaggta cacccagggg acttcggact
      481 tggtgggcat ggcgtgggat aaccatgagc aagaaccggg ccagcgcaaa ctctggcccc
      541 ttgccgatgc aacgcgcggt cgtggtgcga tcgcgtgtcg ccatgtccgt gacatcctcg
      601 cgtgggtcgc cggagccaca ggtgtaccga gcgcgcgaag gtggctcgcc gccgcagcct
      661 cggcgccgca gctggagcgc tggcgcgggc agctcacggc gcagggcacc atcgtcgcta
      721 cgacgccagg gccaccagcg gccttgaccg cggcgctgcc cgcttgccag gcggatcagg
      781 tcacgcgctg ctcggatacc gggcgtgtgc gcgtgcagcg gtcgcgtggc tccacccgca
      841 gacttatgcc catgtcgacg tcggcgtgac gcagatgtcg atcgacgccg ttgtcaccgc
      901 gggcaaccga ccgccgagcg tttatctgtc cgcgacgaac gtccgcgagc acgactgcaa
      961 cgcaccgctg ccagaccgtc gttcatcacc gatgccgagc gcgacgtgtc acgcccgatg
     1021 aacgcatcgg cgcgccgtcg gcgacgtgac cctgggcctg cgcgaccgct gtcggtcgcc
     1081 cagcaacagc gttatcgcgg acgcgctggt gcgactcgtc agcgacccgc gcggagaaca
     1141 gcgtcatcga gtccgaggcc tgcgcgatcc gcctgtccca aggaccagcc gtggatctac
     1201 cgtgcaagcc gatttacacc cccctgaccg gccgacggac gcctgaccgg acagacgtcc
     1261 gaggccgcga gggctgccgg acagaaccgg ctcctcgcgc ccgacgccgc aaagttcacg
     1321 gactaacgac cggattatca atcagcccgc ggccgtgcgg ccgcgggtct cgaggatgtc
     1381 ggtgacgagc aacgcggccg ccgcctgggc caacccgaag ccgagcggcc cgacgatcgg
     1441 cagcgcgagc agcagcgtcc acggcgcgcc gaagccgaac atcgcggtgc tgtgcttctt
     1501 catcagcccc ttctgggccg cgtacgcgag gccgcggcgc tcgaagtacg ggtcgagcag
     1561 ctcccagctg agcatgcgcg aggcgaacca cagctgcgct gccggcccga gaaccgcgcc
     1621 gagcaccggc acgaaggtca gcccgaaggt cagcagggtg acgccgatga agtagagcag
     1681 gcgccgcagg ctgctggcga tcgaagtctt gaacgcgacc ccgccggagg cctcgagctc
     1741 ggcggcccgg ccggggtcga cgagcttgag gccggccatg aacacgcgct cgccgaggaa
     1801 cgggaacacc atgttgacga tgaacagcga cagcagcggc gcggccatca tcgccagcgc
     1861 ggtgccgccg atcttcagcg tccacagcag cgcgctcagc cacgccgccg ggttctcggg
     1921 cgtcgggatc aaggcccaga tcgcccagcc gaggccggcc atcagcgcga tcgaggtgac
     1981 gaccaggctc agcgcgaggt gcaagtacac gcggcggacc tcggggctgt cgaccgcgag
     2041 acgaaggcct cgcagcgcca gcgcggctcc accgatgacg gaggaaggca tgggggtgtg
     2101 gttcactgga tctccgagcg atagatgtcc cacgcgccct catcttgcac ggtgctacgg
     2161 gcaaaaaaca gctcgcagcc gtcgcggctc aggtgcgcgc cagcgtcgtg cgcggggccg
     2221 ttgacgcccg gaaccggctg cccaggaccg aagggaacat cgatcgcggg ccgcgtcgcg
     2281 aaccacagct cattgccccc gctgtcgatc gcgcccgtgc gcgtgtagat cagcaccagc
     2341 tcgtgcgcag gtagcgtcgg atcggcctcg ttgcgctccg ggtcgtcgac gttcacgaac
     2401 ggctcggcgc tcggcgccgc gaacggctgg tccggcccag gtcgcgtcgc aaccaccaaa
     2461 cgctgaccac cacccgccgg cgcataataa agcctcaggt catcgctcga gacgtgcggg
     2521 tcgaaggcat ccgtggcacc cggaaattcg agcgccagcg gcgtcagcgg gccgtaattc
     2581 tgacccttgc ggtcgccggt gtagatgcga taccccgggt cgcccggctg cttggtcgac
     2641 agatacgcgc gcgcgtcgac gttcgacagc gccagcttgc cgtcctcccc cgccgtgctc
     2701 agaccgatgt ccttgttgtt ggcgtagctg aattcgaacg gcatcccgac ctcgacccgc
     2761 gaggcccgat aggtgtcctg cgccccgtcg cgatacgacg accacaacaa aatgagcccg
     2821 gtgtagctga gccacatgtc gaggtcgccc tgttgcacgt tgaccggcat ccccagcgcc
     2881 tgcggct
//
LOCUS       DASWNJ010001617         4418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_168536, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001617 DASWNJ010000000
VERSION     DASWNJ010001617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168536"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2692)
                     /locus_tag="VGB85_26860"
     CDS             complement(<1..2692)
                     /locus_tag="VGB85_26860"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0137747.1"
                     /translation="MGRAAGESRARASPSGRGPDPAYATPLTPLTGDTPLPDALIPPA
                     DSIGNTPMASAPTPLEGDLGDPSAMIAPEPEALPAYIGRYKVVRRIGSGGMGVVYAAW
                     DEALNRKVALKVLRTRVGRNKRDTGRKRLQREAQAMAKLSHPNVVAIYDVGMVEDQIF
                     LAMEFVKGQTLTRWLQAERRPWRVVLGVFMQAGRGLSAAHRAGIIHRDFKPDNVLIDS
                     ENRVRVVDFGLARADGGSGLYARPDTYEAITAKPGNSMLDMRITRTGGMTGTPAYMAP
                     EQYLGQPTDARTDQFSFCVALFEGLYGLRPFVGDRVALVREAVLGGHIQDVPKDSDVP
                     LWLLRILIRGLAVRPGERHRSMDDLLARLSDDPRKPMRRWLAIGGAAVVVGTVVLVAR
                     ELRQGDNQRPQAAALALCDTDAALAGVWDPARRQDVAAAFAASGAAAADLQARRVSAV
                     LDTRVDEWREMVRNTCTQAQALARAPTQPLVTCLRRRLGGLRALSDALLRAEPRTVER
                     ALAGAWSLPPSADCADARAPEASWYHGDERPLAELDRAAVLGALGDVAGGLAAARTVT
                     GVAREKDDLRLQAEALHRQATLERAAGDPAAAERSLLGALWAAEASRQDDLVIDSQTL
                     LLEVLGDLGRDDDAIGLWPRLTGVLQRRSDDGRRESAALHAIGRLLLRRGRHAEAATQ
                     LALALRLGERSLSTEHPQVGRLLLDLALAQARLGAHDEAARHLDRAVRLLRSALPGDD
                     PALVQVHLARAEVERRRGRMREAAADYELAGSIVTANHSQDLTALAEVQLGLGEVALA
                     QNQWLAADTRFGQALALRERMHGPTDPSLIEPLIGLCLAATGRGLHAAAVAAGHRALQ
                     IAERPSGASSTGPARLALARALWAQAAHSGRATPAVRGQIL"
     gene            2765..3043
                     /locus_tag="VGB85_26865"
     CDS             2765..3043
                     /locus_tag="VGB85_26865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137748.1"
                     /translation="MRPLATRQRSGPPRPGSRRAFVGALALGLAAASVVGCASAPGSP
                     PRRNGMEAPPPFPVAPDFSLPDALGAPRTLSSLMGPRGLVLVLYRGHW"
     gene            3056..3373
                     /locus_tag="VGB85_26870"
                     /pseudo
     CDS             3056..3373
                     /locus_tag="VGB85_26870"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            3408..4022
                     /locus_tag="VGB85_26875"
     CDS             3408..4022
                     /locus_tag="VGB85_26875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137749.1"
                     /translation="MARSTSSKIFALVAVGALALGTYKLGTAAVDLFAGESAEAITAK
                     RLANQVWIERLPRDDRDMIHHLVLVEDGGDRFGAFGKSSQWRHFVELFRWAREEHRLT
                     LLLPQERRRLDLDVKVWNCEDEAPAPFELCLELTTSKNKVMRYFSRHDWAIDGSDPES
                     LAQLVVAHPELAGALKELPANPQANLDAATFTVADELIDLELAE"
     gene            4024..4284
                     /locus_tag="VGB85_26880"
     CDS             4024..4284
                     /locus_tag="VGB85_26880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY0137750.1"
                     /translation="MVVVELYGLPRLRAGAERVELAAGRLDALLTALARTLPGLVPDI
                     IADGALTEHALVAIDGRSLLVDPHTEIPDGAVLVMISAQAGG"
     gene            complement(4301..>4418)
                     /locus_tag="VGB85_26885"
     CDS             complement(4301..>4418)
                     /locus_tag="VGB85_26885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006476181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY0137751.1"
                     /translation="RLKAAGKPFKVIMTACMRKLLVILNAMVATGTAWSLSP"
BASE COUNT          547 a         1694 c         1582 g          595 t
ORIGIN      
        1 cgaggatctg cccgcgcact gcgggcgtcg cccggccgct gtgggccgcc tgggcccaca
       61 gcgcgcgcgc gagggccagg cgcgccgggc cggtcgagct cgcgccgctc ggtcgctcgg
      121 cgatctgcag cgcgcggtga ccggcggcga ccgccgcggc atgcaggccg cggccggtcg
      181 ccgccaggca cagcccgatc agcggctcga tcagcgacgg gtcggtcggt ccgtgcatgc
      241 gctcgcgcag ggccagcgcc tggccgaagc gggtgtcggc cgccagccac tggttctgcg
      301 ccagcgcgac ctcgccgagg ccgagctgga cctcggccag cgccgtgagg tcctgcgaat
      361 ggttggcggt gacgatcgat ccggcgagct cgtagtcggc ggccgcctcg cgcatgcgac
      421 cgcggcggcg ctcgacctcc gcgcgcgcga ggtggacctg cacgagcgcc gggtcgtcgc
      481 ccggcaaggc ggatcgcagc agtcggacgg cgcggtcgag gtgccgcgcc gcctcgtcgt
      541 gggccccgag ccgcgcctgc gcgagcgcga ggtcgagcag caggcggccg acctgcgggt
      601 gctcggtgct gaggctgcgc tcgccgagcc gcagcgccag ggccagctgc gtcgcggcct
      661 ccgcgtgacg acctcgtcgc aacaacagcc ggccgatcgc gtgcagggcg gccgactcgc
      721 ggcggccgtc gtcgctgcgg cgctgcagca cgccggtgag acgcggccac agaccaatgg
      781 cgtcgtcgtc gcggccgagg tcgccgagca cctcgagcag cagcgtctgg ctgtcgatca
      841 cgaggtcgtc ctggcggctc gcctcggccg cccacagcgc gccgagcagg ctgcgctcgg
      901 ccgccgcagg gtcgccggcg gcgcgctcga gcgtcgcctg gcgatgcagc gcctcggcct
      961 gcaggcgcag gtcgtccttc tcgcgcgcca cgccggtcac ggtccgcgcc gccgccagtc
     1021 cgccggcgac gtcgccgagc gcaccgagca ccgcggcgcg gtcgagctcg gcgagcgggc
     1081 gctcgtcgcc gtgataccag ctcgcctccg gcgctcgcgc gtcggcgcag tcggccgacg
     1141 gcggcagcga ccacgcgccg gcgagcgcgc gctcgaccgt ccgcggctcg gcgcgcagca
     1201 gcgcgtcgct gagggcccgc aggccgccga ggcggcggcg caggcaggtc accagcggct
     1261 gggtgggcgc gcgcgcgagc gcctgcgcct gcgtgcaggt gttgcggacc atctcgcgcc
     1321 actcgtcgac gcgggtgtcg agcaccgcgc tgacgcgtcg cgcctggagg tcggcggccg
     1381 cggccccgga ggcggcgaac gccgcggcga cgtcctggcg ccgggccggg tcccacacgc
     1441 cggccagcgc ggcgtcggtg tcacacagcg cgagcgcggc ggcctggggg cgctggttgt
     1501 cgccctgtcg cagctcgcgg gccacgagca cgaccgtccc gacgaccacc gccgcgccgc
     1561 cgatcgccag ccagcggcgc atgggcttgc gcgggtcgtc ggacagcctg gcgagcaggt
     1621 cgtccatgct gcggtgccgc tcgccgggcc ggaccgccag cccgcggatc aggatccgca
     1681 gcagccacag cgggacgtcg ctgtcctttg ggacatcctg aatatgcccg ccgagcaccg
     1741 cctcgcggac cagcgccacg cgatcgccga cgaacggccg cagcccgtac agcccctcga
     1801 acagcgccac gcagaagctg aactggtcgg tgcgcgcgtc ggtcggctgg ccgaggtact
     1861 gctccggggc catgtacgcc ggcgtccccg tcatgccgcc ggtccgcgtg atccgcatgt
     1921 cgagcatgct gttgccgggc ttcgccgtga tcgcctcgta ggtgtcgggg cgggcgtaca
     1981 gccccgagcc gccgtcggcg cgcgcgagcc cgaagtcgac cacgcgcacg cggttctcgc
     2041 tgtcgatcag cacgttgtcg ggcttgaagt cgcggtgaat gatccccgcg cggtgcgccg
     2101 ccgacagccc gcgaccggcc tgcatgaaca ccccgagcac cacccgccac ggccgccgct
     2161 cggcctgcaa ccaccgcgtc agcgtctgac ccttgacgaa ctccatcgcc aggaagatct
     2221 ggtcctcgac catcccgacg tcgtagatcg ccaccacgtt cgggtgcgac agcttggcca
     2281 tcgcctgcgc ctcgcgttgc agccgcttgc ggcccgtgtc gcgcttgttg cgaccgaccc
     2341 gcgtgcgcag caccttgagc gcgaccttgc ggttgagcgc ctcgtcccac gccgcataca
     2401 cgacgcccat gccacccgag ccgatcctgc gcacgacctt gtagcggccg atgtacgccg
     2461 gcagggcctc cggctccggc gcgatcatgg cagacggatc gccgaggtcg ccctcgaggg
     2521 gcgtcggcgc ggaggccatc ggcgtgttgc cgatcgagtc cgccggaggg atcaacgcgt
     2581 cgggcagcgg cgtgtcgccg gtcagcggcg tcagcggggt cgcgtacgcc gggtcaggtc
     2641 ctcgccccga cggactggcc cgcgcccgtg actcccccgc tgctcgcccc atcgccggtc
     2701 gatcctatac cagccggcgc ttcccggttg ccgcgacgac atccttcccg gtacgctgtc
     2761 cgcgatgcga ccgctcgcca cccgccagcg atccggcccg ccgcggcccg ggtcccggcg
     2821 cgcgttcgtc ggcgcgctcg cgctgggtct cgccgcggcc tccgtcgtcg gctgcgccag
     2881 cgccccgggc tcgccgccgc ggcgcaacgg catggaggcc ccgccgccgt tcccggtcgc
     2941 gcccgacttc agcctgcccg acgccctcgg cgcgccgcgg accctgagct cgctgatggg
     3001 cccccggggg ctggtgctgg tgctctatcg cggccactgg tgaccctttt gccgcgcgca
     3061 gctcggggag ctgcaggccc atcttcagga gttcgccgcc gccggcgtgc acgtcgcggc
     3121 agtatcggcc gacgacctcg acggcgcgaa gacgatggtg caccgcaagg gtttgacctt
     3181 tccggtgctc agcgacccgc agggcgcgtt actcaaggcg taccgtcact gggacgagga
     3241 cgcggggatc ggcctcgcag ggactttcgt gatccggccc gaccgcagca tcgccttcta
     3301 cgaggacgac ggcgagcggt tctaccggcg gccctcgtcg gcgcgggtgc ttgaggtcgc
     3361 gcgcggtctg tagacgccgc acgcaccggg tggcatgctc ggcggtgatg gcacgcagca
     3421 cctcgtccaa gatcttcgcc ctggtcgccg tcggcgccct cgcgctcggc acctacaagc
     3481 tcggcacagc cgcggtcgac ctgttcgccg gcgagtccgc cgaggcgatc accgccaagc
     3541 gcctcgccaa ccaggtgtgg atcgagcggc tcccccggga cgaccgcgac atgatccacc
     3601 atctcgtcct cgtcgaggac ggcggcgacc gcttcggtgc gttcggcaag agctcgcagt
     3661 ggcgtcactt cgtcgagctg ttccgctggg cccgcgagga gcaccgcctc acgctcctgc
     3721 tcccgcagga gcgccgccgc ctcgacctcg acgtcaaggt ctggaactgc gaggacgagg
     3781 ccccggcccc gttcgagctg tgcctcgagc tgaccaccag caagaacaag gtgatgcgct
     3841 acttcagccg ccacgactgg gcgatcgacg gcagcgaccc cgagtccctc gcgcagctcg
     3901 tcgtcgccca ccccgagctc gccggcgccc tgaaggagct gcccgcgaac ccgcaggcga
     3961 acctcgacgc cgccaccttc acggtcgcgg acgagctcat cgacctcgag ctcgccgagt
     4021 aatttggtcg tcgtcgagct ctacggcctc ccgcgcctgc gcgccggcgc cgagcgcgtc
     4081 gagctcgccg cgggccgcct cgacgccctg ctcaccgcgc tggcccggac gctcccgggc
     4141 ctggtgcccg acatcatcgc ggacggggcg ctgacggagc acgcgctcgt cgccatcgac
     4201 gggcgctcgc tgctcgtcga cccgcacacc gagatccccg acggcgccgt gctcgtcatg
     4261 atctccgcgc aggccggcgg ctgagcagtt gtgtctcccg tcaaggagaa agtgaccacg
     4321 cggtgccggt ggcgaccatc gcgttgagga tgacgaggag cttgcgcatg caggcagtca
     4381 tgatgacttt gaagggcttg ccggcagcct tgaggcgc
//
LOCUS       DASWNJ010001618         1646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_698527, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001618 DASWNJ010000000
VERSION     DASWNJ010001618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698527"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VGB85_26890"
     CDS             complement(<1..339)
                     /locus_tag="VGB85_26890"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEY0137752.1"
                     /translation="MFTACHPVLSREARVALTLRLLGGLTTDEIARAFLVPESTVAQR
                     IVRAKRTLADARVPFEVPRGPELAQRLASVLEVLYLIFNEGYAATSGDDWMRPALCDD
                     ALRLGRILAEL"
     gene            complement(424..684)
                     /locus_tag="VGB85_26895"
     CDS             complement(424..684)
                     /locus_tag="VGB85_26895"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEY0137753.1"
                     /translation="MTASDTHRAITATFRIESARLIAGLTRIVRDVGLAEELAQDALV
                     AALQQWPTSGVPDRPGAWLMTTAKHRAIDLLRRNQRIERKHE"
     gene            complement(722..>1646)
                     /locus_tag="VGB85_26900"
     CDS             complement(722..>1646)
                     /locus_tag="VGB85_26900"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY0137754.1"
                     /translation="GRPVRRRCEAGIARPNTTEGDTTVKIMMLIHSDERAESGAPPDP
                     RLLAAMTGYNDALTKAGVLLTCDGLHPSSQGFRLHSRGGEITVIDGPFGAPRDLIAGY
                     WMLDVKTKEEAVAWARRVPIDPASASRIELRPLMEMADFPVQDDESGWREQETDLRGG
                     DPNGPLRAPVPPADGGRKLRYLLMFKADAHSEAGGLPNQRQLTEMGDLMAEMVSNGML
                     LAGEGLRPSAHATRLRFDRGKPTVIDGPFAETKEMIAGFIMMQASSREEALDWATRGM
                     RIHGDGEAEVRRVFDPEDLAAGPEFRQRAAG"
BASE COUNT          216 a          560 c          628 g          242 t
ORIGIN      
        1 gagctcggcg aggatccgcc cgaggcgcag ggcgtcgtca cacagtgcgg ggcgcatcca
       61 gtcgtcgccg gaggtcgccg cgtagccctc gttgaagatc aggtagagca cctcgagcac
      121 cgaggcgagg cgctgcgcga gctcggggcc gcgggggacc tcgaagggca cgcgcgcgtc
      181 ggcgagggtg cgcttggccc ggacgatgcg ctgggcgacg gtcgactcgg ggacgaggaa
      241 ggcgcgggcg atctcgtcgg tcgtcaggcc gccgagcagg cgcagcgtca gggcgacgcg
      301 agcctcccgc gagagcacgg ggtgacaggc ggtgaacacc aggcgcagga ggtcgtcgcc
      361 gaggtcgtcg tcgacggcgg cctcgaggtc gggcgtgacc ggtgcgccgc gctggtgctc
      421 cagctactcg tgcttgcgct cgatgcgttg atttcgccgc aggaggtcga tcgcgcggtg
      481 cttcgcggtc gtcatcaacc acgcgcccgg gcggtcgggc acgccggagg tcggccactg
      541 ctgcaacgcg gcgaccaatg catcctgcgc gagctcctcg gcgagcccga cgtcgcggac
      601 gatccgcgtg aggccggcga tcagccgcgc cgactcgatg cgaaaggtcg ccgtgatcgc
      661 gcggtgggtg tcggaggcgg tcacggaccc cgactagcgc accggcgcgg gtccgcgcaa
      721 gctatccggc ggcgcgctgg cggaattctg gcccggccgc gaggtcctcg gggtcgaaca
      781 cgcggcggac ctcggcctcg ccgtcgccgt ggatccgcat cccgcgcgtc gcccagtcga
      841 gcgcctcctc gcgcgagctg gcctgcatca tgataaagcc ggcgatcatc tccttggtct
      901 cggcgaaggg gccgtcgatc acggtcggct tgccgcggtc gaaccggagc cgggtggcgt
      961 gggcgctcgg ccgcaggccc tcgcccgcga gcaacatgcc gttgctgacc atctcggcca
     1021 tcaggtcgcc catctcggtc agctggcgct ggttcgggag gccgccggcc tcgctgtggg
     1081 cgtcggcctt gaacatcagc agataacgca gcttgcgacc gccgtcggcg gggggcacag
     1141 gggcgcgcag cggaccgttc gggtcgccgc cacgcaggtc ggtctcctgc tcgcgccagc
     1201 cggactcgtc gtcctggacc ggaaagtcgg ccatctccat cagcgggcgc agctcgatcc
     1261 ggctggcgct cgcggggtcg atcggcacgc gtcgcgccca cgcgaccgcc tcctccttcg
     1321 tcttgacgtc gagcatccag tagccggcga tcaggtcccg cggcgcgccg aaggggccgt
     1381 cgatgaccgt gatctcgccg ccgcggctgt gcaggcgaaa gccctgcgag ctcgggtgca
     1441 ggccgtcaca ggtcagcagc acgccggcct tcgtcaatgc gtcgttgtag ccggtcatgg
     1501 cggcgagcag gcgggggtcg ggcggggcgc cggactcggc tcgctcgtcg gaatggatga
     1561 gcatcatgat cttcacggtc gtgtctcctt cggtggtgtt ggggcgggcg atgccggcct
     1621 cacaccgtcg tcgaacgggg cggccc
//
LOCUS       DASWNJ010001619         2319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_665145, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001619 DASWNJ010000000
VERSION     DASWNJ010001619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665145"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VGB85_26905"
     CDS             complement(<1..527)
                     /locus_tag="VGB85_26905"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEY0137755.1"
                     /translation="MQPIDDPNDPRLDRFRDLISRGDRPDLADFFAESEIAVTRLLAS
                     PHFTTRAVLGTAAHLARLPATCPVHPISRDLLKKTLGFDLHRGVIACGIRPPALTPDL
                     SPGACPIGPVLGDSSLAALLARPTFSALLVQGLADPANLGSIVRSARAFAVDLVLLDR
                     RGADPLSRRAIRAAMG"
     gene            complement(839..>2319)
                     /locus_tag="VGB85_26910"
     CDS             complement(839..>2319)
                     /locus_tag="VGB85_26910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137756.1"
                     /translation="SRGGCVVRDDGPPLAQSQLAELSAMLDPGAPAERRHRALLALEA
                     AGGLALLALAGLGATQLEIRARGGGVRRSLTWGARQGAPLLSEKTDPSGPRGTEVILR
                     GAEIDPARARSWLRDVGRFAPGTLLLDGEEINHGLQSYLASEPFRIGPKDTPPTLHGQ
                     VAVALHGEQARVWLLHGGIVSTHLGLSAVPCFDAVVELGATAPVNATAADLRAAVNLH
                     ISSLADIGVGLLMHVAARLPELTQLEVQARIVTLLLQAVRTRRRLRELMQVPMLPSWT
                     PHDHEQPRSARARPARWIALAELPQAKPGSEPPVVLALYPEQDPAAHALPDAPVLRLD
                     AAERSALTELLGIRFRAPPPRSEAGGVGRLRVAMRRGLRRVGQRLAGLLHLGPGAPLP
                     ESALRPDEQVLLEHLRAVLHGSTTAPETVHLCAGRAGPRLTGGPRGLLLVGRDHPDLK
                     ACLLALRRGPAWLYPACVALLAGQAMPATSARATWLRHWQDP"
BASE COUNT          296 a          704 c         1057 g          262 t
ORIGIN      
        1 cccatcgcgg cccggatcgc gcggcggctc agcgggtcgg cgccgcggcg gtcgagcagc
       61 acgaggtcga ccgcgaaggc gcgcgcgctg cgaacgatcg acccgaggtt ggccgggtcg
      121 gcgaggccct gcacgagcag cgcgctgaag gtcgggcgcg cgagcagggc cgcgagcgag
      181 ctgtccccca ggacagggcc gatcgggcat gccccggggg acaggtcggg cgtgagggcc
      241 ggcgggcgga tcccacaggc gatcacgccg cggtggagat cgaagccgag agttttctta
      301 agaaggtcgc gcgagatggg gtggacgggg caggtggcgg gcaggcgggc gaggtgggcc
      361 gcggtgccga gcacggcccg ggtggtgaag tgcgggctgg cgagcaggcg cgtgacggcg
      421 atctcgctct cggcgaagaa gtccgcgagg tcggggcggt cgccgcgaga gatcaggtcg
      481 cggaagcgat cgaggcgcgg gtcgttcggg tcgtcgatcg gctgcacgcg cgcgcacggt
      541 gacgtgtgca ggggcggtgg tcaaccgggg gccgaggacg tcactctgta cgggcgaggc
      601 gggcgagcac ggcgtcgccg gccggtccga ggcatcactg ccgggcccga gggcgtcact
      661 cgcatggttc gggcgggcga tgcacggcgt cgccggccgg tcggaggcat cactgccggg
      721 cccgagggcg ttactcgcat ggttcgggcg ggcgatgcac ggcgtcgccg gccggtcgga
      781 ggcatcactg tggttcgggc tggtgctgcg cagaggcgcc agctggtcga tgttcgcgtc
      841 aggggtcttg ccagtggcgg agccaggtcg cgcgggcaga ggtcgcgggc atcgcctggc
      901 cggcgagcaa ggcgacgcag gcggggtaca gccaggcggg gccgcggcgc agggcgagca
      961 ggcaggcctt gaggtcgggg tggtcgcggc cgactaacaa caggccgcgg ggaccaccgg
     1021 tgaggcgcgg gccggctcga ccggcgcaca ggtgcacggt ctcgggggcg gtggtgctgc
     1081 cgtggaggac ggcgcgcagg tgctcgagca ggacctgctc gtcggggcgc agggcggact
     1141 cggggagcgg ggcgccgggg ccgaggtgca gcaggccggc gaggcgttga ccgacgcgac
     1201 gcaggccgcg gcgcattgcg acgcgcaggc ggccgacgcc gccggcctcg ctgcgcgggg
     1261 gtggggcgcg gaagcggatg ccgagcagct cggtgagggc gctgcgttcg gcggcgtcga
     1321 gccgcaggac gggggcgtcg ggcaaggcgt gggcggcggg gtcctgctcg gggtagaggg
     1381 cgagcacgac ggggggctcg ctgccgggtt tggcctgcgg gagctcggcg agggcgatcc
     1441 agcgggccgg gcgggcgcgc gcggaccggg gctgctcgtg gtcgtgcggg gtccaggacg
     1501 ggagcatcgg cacctgcatc agctcgcgca ggcgacgacg ggtgcggacg gcctgtagta
     1561 gcaacgtgac gatcctggcc tggacctcga gctgcgtcag ctcgggcagg cgggcggcga
     1621 cgtgcatgag gaggccgacg ccgatgtcgg cgaggctcga gatgtggagg ttgacggcgg
     1681 cgcgcaggtc ggcggcggtg gcgttgacgg gggcggtggc gccgagctcg acgacggcgt
     1741 cgaagcaggg cacggcggac aggccgaggt gggtcgagac gatgccgccg tgcagcagcc
     1801 acacgcgggc ctgctcgccg tgcagggcga cggcgacctg accgtggagc gtcggggggg
     1861 tgtccttggg gccgatgcga aacggctcgc tggcgaggta gctctgcagg ccgtggttga
     1921 tctcctcgcc gtcgaggagc agggtgccgg gggcgaagcg gccgacgtcg cggagccagc
     1981 ttcgggcgcg ggcggggtcg atctcggcgc cgcgcaggat gacctcggtg ccgcgcgggc
     2041 cggagggatc tgtcttttcg gacagcagcg gggcgccctg acgcgcgccc caggtcaggc
     2101 tgcgccgcac gccgccgccg cgggcgcgga tctccagctg ggtggcgccg aggccggcga
     2161 gcgcgagcag ggcgaggccg ccggcggcct cgagcgcgag cagggcccgg tgacggcgct
     2221 cggcgggggc gccggggtcg agcatcgccg acagctcggc gagctgcgac tgggcgagcg
     2281 gggggccgtc gtcacgcacg acgcagccgc cgcgcgaga
//
LOCUS       DASWNJ010001620         2998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_168552, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001620 DASWNJ010000000
VERSION     DASWNJ010001620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168552"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VGB85_26915"
     CDS             complement(<1..790)
                     /locus_tag="VGB85_26915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137757.1"
                     /translation="MHRLLRIVPLAACVAALACVIPNPNFDVDSGGATSTSGPQGSTS
                     ESSGTPTTSAASTTGATTTGGVGGDDSSTSGAPSTTTGADTTTDATTGAPQPMTAELR
                     HYTDEDQCDFPFWCVMGMDVNNPSTGENRNVECFDAPFPPPFTVDRVGFVVFASKGGP
                     GAKLEFHTYDEGAQKPTAEPFHAVDIGVVDGKGRRDFMLDPPVVVDAQRFCINVHSGE
                     KFGPQLGLALDHVPAPAGQTFVGIDGPNGCEVPAFTDLVTLEASK"
     gene            936..1421
                     /locus_tag="VGB85_26920"
     CDS             936..1421
                     /locus_tag="VGB85_26920"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0137758.1"
                     /translation="MPNFKIVPHLWYTREAAEAAHFYASIFPDSRVEVYTLPAESPSG
                     PAGTVECVELTLAGQPFMAISAGPLDPFNHAISFVVECEDQGEIDHYWEALGAGGTIE
                     MCGWLRDRYGLCWQITPRGFGEMMKSSDRAAARRAAEAMLKMKKLDIAEIERAFADEA
                     K"
     gene            complement(1432..1569)
                     /locus_tag="VGB85_26925"
     CDS             complement(1432..1569)
                     /locus_tag="VGB85_26925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137759.1"
                     /translation="MLLDRPRGLVLLALAPAPACGGGGGGSSSNIFQAHRFAYALGYP
                     E"
     gene            1706..2203
                     /locus_tag="VGB85_26930"
     CDS             1706..2203
                     /locus_tag="VGB85_26930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137760.1"
                     /translation="MGWRIVERRIGKAGGEKQRTSRQREWDRKYGADNWAIGYVIDDE
                     FVRQEAALDSVYHRSYDRHFAEHPEDLAELIALARVLRNPHAEATTGVDLQVPAIEDY
                     LRRHGLTLQGREVVDIGTWHGGASHPISVRLSPLTIRCAADPERSLEQFWQERKCLAI
                     WTDEA"
     gene            complement(2216..>2998)
                     /locus_tag="VGB85_26935"
     CDS             complement(2216..>2998)
                     /locus_tag="VGB85_26935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137761.1"
                     /translation="PTAAADGVALGLARYPGAAASDAYDASACAIDAGEFMPPTLDAA
                     DALLAALPPEDAQLVGAAPLRQALFGAIDLLPEGGPESPRAVLVITHSAPNCANAAPD
                     TATLLEGLDPGVAPLADLTKTIGLPLFLITVGATPGASPELADKRPDGVDLSVYPQQL
                     NTTASLNPTDEAMLLADLKSLLTVSSDSSCLFSLDPDPDPDLQHIAEVRIGGEPVPGP
                     VADCPSQTGWRQSGPTELELCAGACDLFAKALHIEIDIACNR"
BASE COUNT          462 a          928 c         1157 g          451 t
ORIGIN      
        1 tcttcgaggc ctcgagcgtc acgaggtcgg tgaacgccgg gacctcgcag ccgttcgggc
       61 cgtcgatgcc gacaaaggtc tgacccgcgg gggcagggac atggtcgagg gcgagaccga
      121 gctgtgggcc gaacttctcg cccgaatgaa cgttgatgca gaaccgctgg gcgtcgacga
      181 cgaccggcgg gtcgagcatg aaatcgcggc ggcccttgcc gtcgacgacg ccgatgtcga
      241 ccgcgtggaa cggctccgcc gtgggcttct gcgcgccctc gtcgtaggtg tgaaactcga
      301 gcttcgcgcc cggcccaccc ttgctggcga acacgacgaa gccgacccgg tcgaccgtga
      361 agggcggcgg gaacggggcg tcgaagcact cgacgttgcg gttctcgccg gtgctcgggt
      421 tgttgacgtc catgcccatg acgcaccaaa acgggaagtc gcactggtcc tcgtccgtgt
      481 agtggcgcag ctcggcggtc atcggctgcg gcgcgccggt ggtcgcgtcg gtcgtcgtgt
      541 ccgcgccggt ggtcgtcgac ggggcgccgc tggtgctgct gtcgtcgccg ccgacgccac
      601 cggtcgtggt ggcgcccgtc gtgctcgccg cggaggtcgt cggggtcccg ctgctctcgc
      661 tggtgctgcc ttgcgggccg ctggtcgacg tggcgccgcc ggagtcgacg tcgaagttgg
      721 ggttcgggat gacgcacgcg agggcggcga cgcaggcggc gagcggtacg atccgcagga
      781 gacgatgcat ggccggcaag gtaacacgat gcgtgtctcg cgcatgggct cctcggcacg
      841 gcggtctcga tctttatttt tttacgcgag gaggtgcaac gaccgatgag tttggcgcgc
      901 gcgggcggtc cagatatcac gaccaggaga cccccatgcc gaacttcaag atcgttcccc
      961 atctctggta cacccgcgag gccgcggagg cggcccattt ttatgcatcg atcttcccgg
     1021 actcccgggt ggaggtgtac acgctgccgg ccgagtcgcc gagcgggccg gccggtacgg
     1081 tcgagtgcgt cgagctcacg ctcgccgggc agccgttcat ggcgatcagc gcggggccgc
     1141 tggacccctt caatcatgcg atctcgttcg tcgtcgagtg cgaggatcag ggcgagatcg
     1201 atcattactg ggaggccctc ggggccggcg gcacgatcga gatgtgcggc tggctgcgcg
     1261 accgctacgg gctgtgctgg cagatcacgc cgcgtgggtt cggcgagatg atgaagtcca
     1321 gcgaccgcgc cgcggcccgc cgcgccgccg aggcgatgtt gaagatgaag aagctcgaca
     1381 tcgccgagat cgagcgggcg ttcgccgacg aggcgaagtg acgcgagcgg atcactcggg
     1441 ataacccagc gcgtacgcga agcggtgcgc ctggaagatg ttcgagctgc tgccaccgcc
     1501 gccgccacca catgcggggg cgggggcgag cgcgagcagg acgagaccac gaggccgatc
     1561 gagaagcatg cgatcgctat ctcgtatgtg cgagcgacgc acctgcgatc gcggcgcggg
     1621 gccgcgcgat cgaggtgcac ggcgcgcgga gagtgtggca gcatacgggc atgcgcgaca
     1681 tttcacgggc ccgggaggcg gggtgatggg ctggcggatc gtcgagcggc ggatcggcaa
     1741 ggccggcggg gagaagcaga ggacctcgcg ccagcgtgaa tgggaccgca agtacggcgc
     1801 ggacaactgg gcgatcggct acgtgatcga cgacgagttc gtgcgccagg aggccgcgct
     1861 cgactcggtg tatcaccgca gctacgatcg acacttcgcg gagcaccccg aggacctcgc
     1921 ggagctgatc gcgctggcgc gggtgctacg aaacccgcac gcggaggcga ccaccggggt
     1981 cgacctgcag gtgccggcga tcgaggacta tctgcggcgg catggattga ccctgcaggg
     2041 ccgcgaggtc gtcgacatcg gcacgtggca cggcggcgcg tcgcacccga tcagcgtgcg
     2101 cctcagcccg ctgacgatcc gctgcgcggc cgatccggag cgctcgctcg agcaattttg
     2161 gcaggagcga aagtgcctcg ccatatggac ggacgaggcc tgacgcgctc gcggatcaac
     2221 gattacaggc gatgtcgatc tcgatgtgga gggccttcgc gaacagatcg caggcgccgg
     2281 cgcacagctc gagctcggtc gggccgctct gccgccatcc ggtctgggac gggcagtccg
     2341 cgaccgggcc ggggaccggc tcgccgccga tacggacctc ggcgatgtgc tggaggtcgg
     2401 ggtcggggtc gggatcgagg ctgaagaggc aggacgaatc cgaggagacg gtcagcaacg
     2461 acttgaggtc ggccagcagc atggcctcgt cggtggggtt gagcgacgcg gtggtgttga
     2521 gctgttgcgg gtatacggac aggtcgacgc cgtcggggcg cttgtcggcg agctccgggc
     2581 tggcccccgg ggtcgcgccg acggttatca agaacagcgg gaggccgatg gttttggtga
     2641 ggtcagcgag cggggcgacg ccggggtcga gaccctcgag cagcgtcgcg gtatcagggg
     2701 cagcattggc gcagttcggg gcgctgtggg tgatcaccag gacggcgcgc ggggattcgg
     2761 ggcctccctc gggtagcagg tcgatcgcgc cgaacagcgc ctgtctcagc ggggccgcgc
     2821 cgaccagctg cgcgtcctcc gggggcaacg cggcgagcag cgcgtcggcg gcgtcgagcg
     2881 tcgggggcat gaattcgccg gcatcgatcg cgcatgcgct ggcatcgtac gcgtcggagg
     2941 cggcggcgcc cggatagcgc gccaggccga gcgcgacgcc gtccgcggcg gccgtcgg
//
LOCUS       DASWNJ010001621         2301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_133257, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001621 DASWNJ010000000
VERSION     DASWNJ010001621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133257"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..583)
                     /locus_tag="VGB85_26940"
     CDS             complement(245..583)
                     /locus_tag="VGB85_26940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137762.1"
                     /translation="MAQLDYGDVWVLRCIAKMCGEYVIHGTVNLRTGALHPEHEGVAL
                     RASGNRSTSLVADHVKRLLLHGLVVGKELGHTVNEKDRDHSLKVNTPTELGERLLALV
                     ADRESTSPAA"
     gene            881..2104
                     /locus_tag="VGB85_26945"
     CDS             881..2104
                     /locus_tag="VGB85_26945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137763.1"
                     /translation="MSRLTVAAVTLSALLPACTTIIVQAPDDEDGSTSAVSFGSWPAP
                     DDASSTSGELDEDQTTGEHVDGSTSGEIDDTSLTGDTSTGEIDGTTSGSAGDESTSTG
                     SSGGDTTSSTSTGDTGSEAMLEPVGCAPAAVGVNAAFTGAIQGNCGCHGGGAGGLTFN
                     DTASFKAATVNVMATNADMQRITPGDINESYLLYKLHGQQLKVGGGSQMPFGGPQLSD
                     TAQCLMINWVKGLSTAKALGEVCGADDECESGACIGSGLEDFNRCSTPCTLDAADDCS
                     KQGHPGICLWAGGDQHWCGGSFPTDATFFGIATPPPPNSQPWSTSGQIANPVARHFWL
                     IPAWSSPFFVKTASLDFGPHAVDIIASNGTVIESFETDVDHFSIAPQGVDKYHWLVLR
                     PLGDKSHYAMWLDSE"
BASE COUNT          441 a          784 c          705 g          371 t
ORIGIN      
        1 agtcgccgcc gaaggtgacg gactccgcga aaccatccgt cgcataccaa accggtattg
       61 gatcgcctct tcaccagaac ccgcagaaca accccaaccc cctacagaag cccgcgcagg
      121 cccgcaacgg cgcggttttg acggtcgggc gagttaccct acaggtacct cgcccgaccc
      181 tgttttgctc cccggtactg gcacgccagt gtcactgaac gcgacagtgg aaccagaatg
      241 gagatcaggc cgccgggctg gtgctctcgc ggtcggcgac cagcgccagc aagcgctcgc
      301 cgagttcggt cggcgtgttg accttcaacg agtggtctcg atccttctcg ttgaccgtat
      361 ggcccaactc tttacctacc acaagcccgt gaagaagtag ccgcttcaca tgatctgcta
      421 ctagagaagt tgacctattc ccactcgctc gcaaggcgac gccttcgtgc tccgggtgaa
      481 gagcgcccgt tctgagattg acggtgccgt ggatgacgta ttcaccacac atcttggcga
      541 tgcaacgcag aacccatacg tcaccataat cgagttgcgc caagatgctg agcaggcgca
      601 gggtcagtcc ttcctcgaca tgacgcgccc tgagaaccag agggactggg ctggagccct
      661 cgcagccgcg gtctcttcga ccgcatcgtc tgcgttgctt tcgatgcgcg tacgcaaggc
      721 tcgccggacc acgttgtcgc gaccatgttt tcggtgagtt ccagcgaacg gcgcgatcgt
      781 cgcgaacgcg gtacccgctc gactctcgcg tacggcagag ctactactgg tctcttcgtc
      841 cacctgaccc aaaggtcagg tggcaaggag ccgccagccc atgtctcgtc tcaccgtcgc
      901 cgccgtcacc ctgtctgccc tcctgcccgc ttgcacgacg atcatcgtcc aggcaccgga
      961 cgacgaggac ggctcgacga gcgccgtgtc gttcggctca tggcccgcac cggacgacgc
     1021 gtcgagcacg agcggcgagc tggacgaaga tcagaccacg ggtgagcacg tcgacggctc
     1081 gacctccggg gagatcgacg acaccagctt gaccggcgac acctcgaccg gagagatcga
     1141 cggcaccacg agcggcagcg cgggcgacga gtcgacctcg accgggagca gcggcggcga
     1201 cacgacctcg agtacgagca ccggtgacac cggtagcgag gcgatgcttg agccggtcgg
     1261 gtgcgccccg gcggcggtcg gggtcaacgc ggccttcacc ggcgcgatcc agggtaactg
     1321 tggctgccac ggcggcggcg ctggcggcct caccttcaac gacacggcca gcttcaaggc
     1381 tgccaccgtc aacgtgatgg ccaccaacgc cgacatgcag cgcatcacgc cgggcgacat
     1441 caacgagagc tacctgctct acaaactgca cggccaacag ctcaaagtcg gcggcggcag
     1501 tcagatgccc ttcggcggcc ctcagctcag cgacactgcg cagtgcttga tgatcaactg
     1561 ggtgaagggc ctcagcacgg cgaaggcact cggcgaggtg tgcggtgctg acgatgagtg
     1621 cgagagcggc gcctgcatcg gctcaggcct cgaggacttc aaccggtgct cgaccccgtg
     1681 cacgctcgac gcggccgacg actgctcgaa gcaggggcat ccgggcatct gcctctgggc
     1741 cggcggagat cagcactggt gcggtgggtc gttcccgacc gacgccacgt tcttcggcat
     1801 cgcaacgccg ccgcctccga acagccagcc atggtccacg tcggggcaga tcgccaaccc
     1861 ggtcgctcgt cacttctggc tcatccccgc gtggtcgtct cccttcttcg tgaagaccgc
     1921 ctcgctcgac ttcggcccgc acgcggtcga catcatcgcc agcaacggca ccgtcatcga
     1981 gagcttcgag accgatgtcg atcacttctc gatcgcgccg caaggcgtcg acaaatacca
     2041 ctggctcgtg ctccgcccgc tcggcgacaa gtcgcactac gccatgtggc tcgacagcga
     2101 gtgacaacct gagccgcatg agcgccggct gggtgcgctc gtagagccga tagcagcggc
     2161 cgcatggtgc ggctgcttgg agatgtcatg tccggcaacg aacagaaacg cgagcgccaa
     2221 gcgcccgcgg caggagaaat cacgtgcatc agctcgaccg aatcgaatgc tacgaacaac
     2281 tggcccagga gtggagcaag c
//
LOCUS       DASWNJ010001622         2703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_240901, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001622 DASWNJ010000000
VERSION     DASWNJ010001622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240901"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VGB85_26950"
     CDS             <1..710
                     /locus_tag="VGB85_26950"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137764.1"
                     /translation="DFVARAWLGNKSAGEHRFKGRTTEQSLSFVAMPALLKEGAADLV
                     LAKDGPGRLYYRLGLRYAPKDLTLAPAEYGFVVDRKYEAIDDPKDVRRDPDGTWRIKA
                     GARVKVNLTMVAQDRRYHVALVDPLPAGLEAVNPALATSGKLDPSQDHGGPRPIDSMP
                     FKRGWWWWREWYDHQNLRDERVEAFTTLLWEGVHDYSYTARSTTPGTFVVPPAKAEEM
                     YAPETFGRSGSDRVIVE"
     gene            789..1436
                     /locus_tag="VGB85_26955"
     CDS             789..1436
                     /locus_tag="VGB85_26955"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEY0137765.1"
                     /translation="MHDPAEEARERMVDEQIVARGIADPAVLAAMRTVPRHRFVPGVH
                     LRVAYADHPLPIGHGVTISQPYIVALMTELAQLGPGARVLEIGTGCGYQTAVLAALGA
                     RVFSVEIIDELAAQAAATLTALGFGDISLRRGDGHAGWPEHAPFDAIVVTAAPPVVPP
                     PLLAQLAPGGRLVIPVGRGIQDLQRITRHGDTFSTERVAPVQFVPMTGAAQNSGS"
     gene            1808..2476
                     /locus_tag="VGB85_26960"
     CDS             1808..2476
                     /locus_tag="VGB85_26960"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY0137766.1"
                     /translation="MPVLPLPNVVLLPGMVLPLNVHEPQYLELVDFVRAHGMYVGVPL
                     LLPPTPGGETPALAPVFGLGKLVFHVELSDGRRIIRLEGVGRVRIQRELPQSHRFREL
                     AVSPLSEPYPTDPEPITVLKAQVERITQYCGEDGEGLQSLLSIRDERTFLYSLTAFLP
                     SLELLVCDESEVVANDRALVTLQQRSLAAETADERVDFLLERTSGIVARLGEQFMSAA
                     RLLN"
BASE COUNT          411 a         1067 c          845 g          380 t
ORIGIN      
        1 cggactttgt cgcccgcgcc tggctcggca acaagtcggc gggcgagcac cgcttcaagg
       61 gccgcaccac cgagcagtcg ctcagcttcg tggcgatgcc cgccctgctc aaggagggcg
      121 ccgccgacct cgtgctcgcc aaggacggcc ccggccgcct gtactaccgc ctcggcctgc
      181 gctacgcccc gaaggacctc accctcgcgc ccgccgagta cggcttcgtc gtcgaccgca
      241 agtacgaggc gatcgacgac ccgaaggatg tccgccgcga ccccgacggc acctggcgga
      301 tcaaggccgg cgcgcgcgtc aaggtcaacc tgacgatggt cgcccaggac cgccgctacc
      361 acgtcgccct cgtcgacccg ctgcccgccg gcctcgaggc ggtcaacccc gccctcgcca
      421 cctcgggcaa gctcgacccc tcgcaggacc acggcggccc gcgcccgatc gacagcatgc
      481 cgttcaagcg cggctggtgg tggtggaggg agtggtacga ccaccagaac ctgcgcgacg
      541 agcgggtcga ggcgttcacc accctgctgt gggaaggcgt gcacgactac agctacacgg
      601 cgcgctcgac caccccgggc accttcgtgg tcccgccggc caaggccgag gagatgtacg
      661 cgcccgagac cttcgggcgc agcggcagcg accgcgtcat cgtcgagtga accccgatcc
      721 gcgcgtcacc catgacggca tgcacacggg ttgcgcccgc ggcacgaccc cgcatacgat
      781 cccccgacat gcacgacccg gccgaggaag cgcgcgagcg catggtcgac gagcagatcg
      841 tcgctcgtgg gatcgccgac ccggccgtgc tcgcggccat gcgcacggtg ccgcgccacc
      901 gcttcgtgcc cggcgtgcac ctgcgcgtcg cttacgccga ccacccgctc ccgatcggcc
      961 acggcgtcac gatcagccag ccatacatcg tcgcgctgat gaccgagctc gcgcagctcg
     1021 gccccggcgc gcgcgtgctg gagatcggca ccggctgtgg ctaccagacg gcggtcctcg
     1081 ccgctctcgg cgcgcgggtg ttcagcgtcg agatcatcga cgagctcgcc gcccaggccg
     1141 ccgccaccct caccgccctc ggcttcggcg atatctcgtt gcggcgcggc gacggccacg
     1201 ccggttggcc ggagcacgcg cccttcgatg cgatcgtcgt caccgccgcc ccgccggtcg
     1261 tgccaccccc gctgctcgcc cagctcgcgc ccggcggccg cctcgtgatc ccggtcggcc
     1321 gcggcatcca ggacctccag cgcatcaccc ggcacggcga caccttctcc acggagcgcg
     1381 tcgcgccggt gcagttcgtg ccgatgaccg gcgccgccca gaactcggga agctgactcg
     1441 ggggcgaccg gccacgatcc acgaaacagc gtctcccgtg cgccgcagtg cccgcagcgg
     1501 ccaggtctcg ccattcggtc gcccgacgcg cagcaacgcg atccgcgacc gagaaaccgg
     1561 ggacgcaaaa tcatcgcgcc cccaggcgag acgcgacgac aaaaaacgca gcgatccacc
     1621 gcctcgccaa acgcccccca gaaccctccg cgaccacgtt cacgcgataa ttccagcatc
     1681 gagtggcgtt cccctgcgca tgaagcgctc ggggtgaatc cctggcacat ccacgggcga
     1741 aggctataga tgaatcgttc gtatgcggaa cgtgcactcc atcccgggga ccgatgtgtt
     1801 ggcgtccttg ccggtcctgc cgctgccgaa cgtcgtgctg ctgcccggga tggtgctgcc
     1861 gctcaacgtc cacgagccgc aatacctcga gctcgtcgac ttcgtgcgcg cgcacggtat
     1921 gtacgtcggc gtgccgttgt tgctgccgcc gacccccggc ggcgagaccc cggcgctcgc
     1981 cccggtgttc ggcctcggca agctggtgtt ccacgtcgag ctctccgacg gccgccgcat
     2041 catccgcctc gagggtgtcg gccgcgtccg catccagcgc gagctgccgc agagccatcg
     2101 cttccgcgag ctcgccgtca gcccgctgtc cgagccctac ccgaccgacc ccgagccgat
     2161 caccgtgctc aaggcccagg tcgagcgcat cacccagtac tgcggcgagg acggcgaggg
     2221 cctgcagtcg ctgctcagca tccgcgacga gcgtaccttc ctctactcgc tgaccgcctt
     2281 cctgccgagc ctcgagctgc tcgtctgcga cgagtccgag gtcgtcgcca acgaccgcgc
     2341 cctcgtcacg ctccagcagc gcagcctcgc ggccgagacc gccgacgagc gcgtcgactt
     2401 cctgctcgag cgcaccagcg ggatcgtcgc ccgcctcggc gagcagttca tgagcgccgc
     2461 gcggctgctc aactgacagt ggcacctgtc cctgctgtcc ttgctgtcct tgctgtccca
     2521 tgagacagcc cggagcgcgc ccggtcgtcg cccccctggg cgcggatcgg cgcatcctgc
     2581 acactggagt taatactgga gtgaagcgtc ttccaggcct cagcgcccgc cggggcgcca
     2641 ttcgctgcat acccgtagaa ccccgcaaac ccgcgaatac gcgcgcagcc acgaggctca
     2701 ctt
//
LOCUS       DASWNJ010001623         6509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_450671, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001623 DASWNJ010000000
VERSION     DASWNJ010001623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6509
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450671"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="VGB85_26965"
     CDS             <1..1119
                     /locus_tag="VGB85_26965"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0137767.1"
                     /translation="YAGWGAAAKVLQLEARTPELAQTTAAAGTSWLSVDAMAVVKATQ
                     ALSGELVQDRLLGNLMHVVLEHAGAQTGHLLLVEHDRLVIAAAAHTGSEGVEMSLPSP
                     RTPVRGDELALSIVQYVRRTREHVLLVDASADPAFASDPYLARVQVRSALCLPILRSG
                     ALLGVLYLENNWMAGAFTPQRIAVLDLLLAQAAISLEVTTLYADLDRENRERRVIEQG
                     LRESEDRLRQLVDSTQRQLEIIASQREAIRRLSTPIIQVWQGVVMMPLFGSIDHGQAI
                     QMQETLLEAIVRIRVRSAIIDLTGVDIIDTTTAQHIIGLVQAVQMLGARAIIVGIRPD
                     VAQSIVALGVDLAGIVTLSNVHEALLLCMQAPRLRTRG"
     gene            1230..1727
                     /locus_tag="VGB85_26970"
     CDS             1230..1727
                     /locus_tag="VGB85_26970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137768.1"
                     /translation="MPAVPPVDWDRLARRVALYSWAQVGFLMILVGSLHLFPLLGMPW
                     SSCLVGATGATDWACVAFFHLYEIPFVALFVHHAYVGFTRMRRSPRTSLPAYTLLTLF
                     QVIMLVVFLTFESKTLLDGLAQQVPLWEQVVVYGGCVGILVDALLGFHVVFACLVPAW
                     IAERR"
     gene            1776..3350
                     /locus_tag="VGB85_26975"
     CDS             1776..3350
                     /locus_tag="VGB85_26975"
                     /inference="COORDINATES: protein motif:HMM:NF015963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HEY0137769.1"
                     /translation="MSPNATRPLARLLRPETDGFYHPNDEHEVAALIRMARSRGLQLR
                     VRGSAHSEAAAIYSDARLQGAGEAVDVVLDRLDGVRFDDARQQVVVAAGCRFGHDPRD
                     ITGRATEARGLCAQLDARGWALPILAGVSHQTVAGFFATGSCGGSVQHALDRAIVGLR
                     LIDGSGQMHVLTPETTPELFAAAGVALGLLGVVVEVTLQCEPRFDIVGEEAITDRADA
                     AVDLFAETGPRSVASFLRAHSYARLLWWPQPGVDKLVVWTARREPGASGEPRPYRAFP
                     RLFGSDAMIQRLAGLGMRGLDAWQTAAQALPRALRAATDPLRGALVNAFVPTDSAGPQ
                     QFRDVWWRALPMDDQIDERWMPVDFVEIWLPIDRAAEAMSRLRTMYEQTGWDLAVNAP
                     VEIYAGATSRFWLSPGHGGERVRINVFWPRFNRDDPNAGSFAAHVACLAELGARFHWA
                     KHVPACFGPAAAEAAYPQWDAFMALRARLDPDQLFLTGRWRRITGVAPDGAAAPRRRL
                     ELVASADSHCAHWGSE"
     gene            complement(3418..4461)
                     /locus_tag="VGB85_26980"
     CDS             complement(3418..4461)
                     /locus_tag="VGB85_26980"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEY0137770.1"
                     /translation="MRAQPVTILGFLSVLGVACAEGSQAPPAREAGPQPGGLPAAAAA
                     PARQISEYIVELYEDRAGNIWFGTISDGVCRYDGESFVCFTSRDGLGGGSVRGIVEDE
                     AGDLWFATDSGVFRRDGTSFTRYTVADGLGDDRVLTVARDRRGTLWFGSDGGVTRYDG
                     TTFSNLALPLVASLHKDFPTGNRSPALVWSILEDRSGALWFGTNGSGVIRYDGAAFTR
                     FTTLEGLSEDFVYSIHEDRRGILWFGTSGGLSRHDGTTFTTYSERDGLGGHDVWAIVE
                     DRGGDLWLGTGGGGVTRFDGATSFQTISTQQGLGNGHVQSLLEDSRGRLWIGTSGGAY
                     RLDGTRAVNVTRE"
     gene            4585..5055
                     /locus_tag="VGB85_26985"
     CDS             4585..5055
                     /locus_tag="VGB85_26985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0137771.1"
                     /translation="MELRRIVLIEDSPNDARLTLTAFRQSGMANEVVWLKDGQEAVDY
                     LFRTGAHADRPPGHPAVVLLDIKLPKLDGLQVLEQIKADPELRMVPVVMLTSSNQEGD
                     LSRSYGLGVNAYVVKPVGFPEFVQALKELGLFWAVVNCPPPPPTPVPRPVRGAT"
     gene            5052..>6509
                     /locus_tag="VGB85_26990"
     CDS             5052..>6509
                     /locus_tag="VGB85_26990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0137772.1"
                     /translation="MTAAQEAQLAAVGPGNDVSALRVLLLEDSRLDEKIITAQLRQRG
                     LECTLQRVDARAPFLTALEDWRPDIVLSDYNVPGFGGEDALRITRERHPDLPFLFVSG
                     ALGEHAAVELLKRGATDYVLKDRLERLVPSIERALRESREKAERLQAEERLRERERTL
                     STLMRNLPGMAFRARMRRPWYLDFASEGCLALTGYEPEAFRPGGRTDFDSIMHPDDAQ
                     RVAAEAEAAHAGGEQLTTTYRIRTLQGEERWVWVRAVTHPGPDGDAPVIEGFVTDITP
                     QKLAEAEILRRIEFEQQLIGIVSHDLKNPLQAILFGATTLLKREAIDEVGTRLARRIL
                     ASTERATRMINDLLDFTQIRLGAGLPVSPRDADLRDLTAQVLDEFEHTHPDRRLVFVA
                     PPALRAWIDPGRVTQAVTNLVQNALTYSPRDTPVSIRLEVDGDDAVIEVHNTGEPIAP
                     DKLADLFEPLRRGVAVTSAHTRSIGLGLFIVASIVR"
BASE COUNT          919 a         2449 c         2192 g          949 t
ORIGIN      
        1 tacgccggct ggggcgccgc ggccaaggtc ctccagctcg aggcccggac ccccgagctc
       61 gcccagacga ccgccgccgc cggtacgagc tggctgtcgg tcgacgcgat ggcggtcgtc
      121 aaggccacgc aggcgctcag cggcgagctc gtccaggacc gcctgctcgg caacctgatg
      181 cacgtcgtgc tcgagcacgc cggcgcgcag accgggcacc tgttgctcgt cgagcacgac
      241 cgcctcgtga tcgccgccgc cgcccacacc ggcagcgagg gcgtcgagat gtcgctgccc
      301 tcgccgcgca cgccggtccg cggcgacgag ctcgccctgt cgatcgtgca atacgtccgc
      361 cgcacccgcg agcacgtgct gctcgtcgac gccagcgccg acccggcctt cgccagcgac
      421 ccgtacctgg cgcgcgtgca ggtccgcagc gcgctgtgcc tgccgatcct gcgctcgggc
      481 gcgctgctgg gcgtgctcta cctcgagaac aactggatgg ccggcgcctt cacgccgcag
      541 cggatcgccg tgctcgacct gctgctcgcc caggccgcga tctcgctcga ggtcaccacc
      601 ctctacgccg acctcgaccg cgagaaccgc gagcgccgcg tgatcgagca gggcctgcgc
      661 gagagcgagg accggctgcg ccagctcgtc gacagcaccc agcgccagct cgagatcatc
      721 gcctcccagc gcgaggcgat ccgccggctc tcgaccccga tcattcaggt ctggcagggc
      781 gtggtcatga tgccgctgtt cggcagcatc gatcacggcc aggcgatcca gatgcaggag
      841 acgctgctcg aggcgatcgt ccgcatccgc gtgcgctcgg cgatcatcga cctcaccggc
      901 gtcgacatca tcgacaccac caccgcccag cacatcatcg gcctcgtcca ggcggtccag
      961 atgctcggcg cccgcgcgat catcgtcggc atccgcccgg acgtcgcgca gtcgatcgtc
     1021 gcgctcggcg tcgacctcgc tggcatcgtc acgctctcga acgtgcacga ggcgctgctg
     1081 ctatgcatgc aggccccccg gctgcgcacc cgcggatagg atccatagcg gcccgggcgg
     1141 cccagtcctc gctcgtcgcc tcgggccagt ccgtgctacg ggacgcgatg tccgtcagtc
     1201 cggcgcctcc catcgctcca gacgcagccg tgccggcggt cccgcccgtc gactgggacc
     1261 gcctggcgcg ccgggtcgcc ctgtattcgt gggcgcaggt cggcttcctg atgatcctgg
     1321 tcggctcgct gcacctcttt ccgctcctgg gcatgccgtg gtcctcgtgc ctggtcggcg
     1381 cgacgggggc gaccgactgg gcctgcgtcg cgttcttcca cctctacgag atccccttcg
     1441 tcgcgctgtt cgtccaccac gcgtatgtcg gtttcacgcg catgcggcga tccccacgga
     1501 cgtcgctgcc cgcctacacc ctgctgaccc tgttccaggt gatcatgctg gtcgtgttcc
     1561 tcaccttcga gtcgaagacg ctgctcgacg gcctcgcgca gcaggtgccg ctgtgggagc
     1621 aggtcgtcgt ctacggcggc tgcgtcggca tcctcgtcga cgcgctcctc ggcttccacg
     1681 tggtgttcgc ctgcctcgtg cccgcgtgga tcgcggagcg ccgatagagc tcccggcccg
     1741 cgatcgttcg gcccgcgagg gtcacttcgg cgtcgatgtc cccaaacgcc acccgcccgc
     1801 tcgcccggct cctgcgaccc gagaccgacg gcttttacca cccgaacgac gagcacgagg
     1861 tcgccgccct gatccgcatg gcccgcagcc gcggcctcca gctgcgcgtg cgcggctcgg
     1921 cgcactccga ggcggccgcc atctacagcg atgcgcgcct gcaaggcgcc ggtgaggcgg
     1981 tcgatgtcgt gctcgaccgc cttgacggcg tccgcttcga cgacgcgcgc cagcaggtcg
     2041 tcgtcgcggc cggctgtcgc ttcggacatg acccgagaga catcaccggg cgggccacgg
     2101 aggcgcgggg cctgtgcgcg cagctcgacg cgcgcggctg ggcgctgccg atcctcgccg
     2161 gggtctcgca ccagaccgtc gccggcttct tcgccaccgg atcgtgcggc gggtcggtgc
     2221 agcacgcgct cgaccgcgcg atcgtcggcc tgcggctgat cgacggctcc gggcagatgc
     2281 acgtgctgac gccggagacc accccggagc tgttcgcggc cgcgggcgtc gccctcgggc
     2341 tgctcggcgt cgtcgtcgag gtcacgctgc aatgcgagcc gcgcttcgac atcgtcggtg
     2401 aggaggcgat caccgaccgc gccgacgcgg cggtcgacct gttcgccgag accggcccga
     2461 ggtccgtggc gagcttcctg cgcgcgcaca gctacgcgcg cctgctgtgg tggccgcagc
     2521 cgggggtcga caagctggtg gtctggaccg cgcggcgcga gccgggggcg tcgggtgagc
     2581 cgcgcccgta tcgcgcgttc ccgcggctgt tcgggtccga cgcgatgatc cagcggctcg
     2641 cggggctcgg catgcgcggg ctcgacgcct ggcagaccgc cgcgcaggcg ctgccgcgag
     2701 cgctgcgagc cgcgaccgac ccgctgcgcg gcgcgctggt caacgccttc gtgccgaccg
     2761 acagcgccgg tccgcagcag tttcgcgacg tgtggtggcg agcgctgccg atggacgacc
     2821 agatcgacga gcgctggatg ccggtcgact tcgtcgagat ctggctgccg atcgatcgcg
     2881 cggccgaggc gatgagccgg ctgcgcacga tgtacgagca aaccggctgg gacctcgccg
     2941 tcaacgcgcc agtcgagatc tacgccggcg cgaccagccg gttctggctc agccccgggc
     3001 acggcggcga gcgggtacgg atcaacgtgt tctggccgcg cttcaaccgc gacgacccga
     3061 acgcgggcag cttcgcggcg cacgtcgcct gcctcgccga gctcggcgcg cgcttccact
     3121 gggccaagca cgtgccggcc tgcttcgggc cggccgcggc cgaggccgcc tatccgcagt
     3181 gggacgcgtt catggccctg cgcgcgcgcc tcgaccccga ccagctgttc ctcaccggac
     3241 gctggcggcg gatcacgggc gtcgcgcccg acggcgccgc ggcgccgcga cgacgactcg
     3301 agctggtcgc gtccgccgac tcgcattgtg cacactgggg atcggagtga gcccgggcca
     3361 caccatgcac catggtgccg gcgcagcgtc cacggagctc gccgcgacga cggcacgtca
     3421 ctcgcgcgtg acgttgaccg cccgcgtacc atcgaggcga tacgcgcccc cggacgtacc
     3481 gatccacagg cgccccctgc tgtcctccag caggctctgg acgtggccat tgccgagacc
     3541 ctgctgcgtc gagatcgtct ggaagctcgt cgcgccgtcg aagcgggtca caccgccgcc
     3601 accggtgccg agccagaggt cgccgccgcg atcctcgacg atcgcccaga cgtcgtgacc
     3661 gccgagccca tcgcgctcgc tgtaggtcgt gaacgtggtc ccgtcgtggc gggacaggcc
     3721 gccgctggtg ccgaaccaca ggatcccgcg ccggtcctcg tgaatgctgt agacaaaatc
     3781 ctcgctcaat ccctcgagcg tcgtgaacct cgtgaacgcc gcgccgtcat atcggatcac
     3841 gccgctcccg ttcgtgccga accacagagc gcccgagcga tcctcgagga tgctccagac
     3901 cagcgccggc gaacgattcc ccgtcgggaa atccttatgt aaggaggcca ccagcggcaa
     3961 cgcgaggttc gagaaggtcg tgccgtcata tcgtgtgaca ccgccgtcgc tgccgaacca
     4021 tagcgtgccg cgacgatccc gggcgacggt caggaccctg tcgtcgccga ggccatccgc
     4081 caccgtgtac cgcgtgaacg acgtgccatc ccggcgaaag acgccgctgt cggtggcaaa
     4141 ccatagatcg ccggcttcat cctcgacgat gccgcggacc gaccctccgc cgaggccgtc
     4201 acggctcgta aaacatacga aggactcgcc gtcgtaacga cagacgccgt cggagatcgt
     4261 gccgaaccaa atgttgcccg cccggtcctc gtacagctcg acgatgtact cgctgatctg
     4321 tcgcgccggg gcggccgccg cagcggggag accaccgggc tgcggtcccg cctcccgtgc
     4381 cggcggcgcc tgcgatccct cggcacaggc gacgccgagc acgctcagaa aaccgaggat
     4441 cgtgacgggc tgtgctcgca tcgtgcgcct catcatatac aaccagcgca gcgcgcgcct
     4501 caccgcgcgc ggtcgcgtcc cgaccgatcc ggccaggtat ccgcgtgatc gacgggcggg
     4561 cctttcgggg gctataaccg tcagatggaa ctccggcgga tcgtgctcat cgaggacagc
     4621 cccaacgacg cgcggctgac actgacggcg tttcgccaga gcggcatggc caacgaggtc
     4681 gtgtggctca aggacgggca ggaggcggtc gactacctgt ttcggaccgg ggcgcacgcc
     4741 gaccgcccgc cggggcatcc ggcggtggtg ctcctcgaca tcaagctgcc caagctcgat
     4801 ggcctgcagg tgctcgagca gatcaaggcc gaccccgagc tgcgcatggt gccggtcgtc
     4861 atgctgacca gcagcaacca ggagggcgac ctctcgcgct cgtacggcct cggcgtcaac
     4921 gcgtacgtcg tcaagccggt cggcttcccc gagttcgtcc aggcgctcaa ggagctcggg
     4981 ctgttctggg ccgtcgtcaa ttgcccgccc ccgccgccga cgccggtgcc gcggccggtc
     5041 cgcggcgcca cgtgaccgcc gcgcaggagg cccagttggc tgcggtcggc cccgggaacg
     5101 acgtgtccgc gctgcgcgtg ctgttgctcg aggacagccg cctcgacgag aagatcatca
     5161 ccgcccagct gcgccagcgc ggcctcgagt gcaccttgca gcgcgtcgac gcccgcgcgc
     5221 cgttcctgac ggcgctcgag gactggcggc ccgacatcgt gctctcggac tacaacgtcc
     5281 ccggcttcgg tggtgaggac gcgcttcgca tcacccgcga gcgtcacccc gacctgccgt
     5341 tcctgttcgt ctcgggcgcg ctcggcgagc acgcggcggt cgagctgctc aagcgcggcg
     5401 ccaccgatta tgtcctcaag gacaggctcg agcggctcgt cccgagcatc gagcgcgcgc
     5461 tgcgagagtc gcgggagaag gccgagcgcc tgcaggccga ggagcggctg cgcgagcgcg
     5521 agcggacgct gtcgacgctg atgcgcaacc tgccgggcat ggcctttcgc gcgcgcatgc
     5581 gccggccgtg gtacctcgac ttcgccagcg agggctgcct cgcgctgacg ggctacgagc
     5641 cggaggcgtt tcgtcccggc ggtcggaccg acttcgactc gatcatgcac ccggacgacg
     5701 cccagcgggt cgccgccgag gcggaggcgg cccacgcggg cggcgagcag ctgacgacga
     5761 cgtaccggat ccggaccctg cagggcgagg agcgctgggt gtgggtgcgc gccgtgaccc
     5821 accccgggcc cgacggcgac gcgccggtga tcgagggctt cgtcaccgac atcaccccgc
     5881 aaaagctcgc cgaggccgag atcctgcggc gcatcgagtt cgagcagcag ttgatcggca
     5941 tcgtcagcca tgacctcaag aacccgctgc aggcgatcct gttcggcgcg acgacgctgc
     6001 tcaagcgcga ggcgatcgac gaggtcggca cccggctcgc gcggcggatc ctcgccagta
     6061 ccgaacgcgc gacgcggatg atcaacgacc tgctcgactt cacccagatc cgcctcggcg
     6121 ccggtctgcc ggtcagcccg cgcgacgccg acctgcgcga cctgaccgcg caggtgctcg
     6181 acgagttcga gcacacccac cccgaccgcc gcctcgtgtt cgtcgccccg ccggcgctgc
     6241 gcgcctggat cgaccccgga cgggtcacgc aggccgtcac caacctcgtg cagaacgccc
     6301 tcacctactc gccgcgcgac acccccgtgt cgatccgcct cgaggtcgac ggcgacgacg
     6361 cggtgatcga ggtccacaac accggcgagc cgatcgcgcc ggacaagctc gccgacctgt
     6421 tcgagccgct gcgccgcggc gtcgccgtca ccagcgccca cacccgcagc atcggcctcg
     6481 gcctgttcat cgtcgccagc atcgtgcgc
//
LOCUS       DASWNJ010001624         2614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_450676, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001624 DASWNJ010000000
VERSION     DASWNJ010001624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450676"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1461
                     /locus_tag="VGB85_26995"
     CDS             <1..1461
                     /locus_tag="VGB85_26995"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY0137773.1"
                     /translation="GFVLVAVEDAVHTPPPRPRHAVVIVVDGLGLAHARPLRSVARLA
                     AAGQCRETDVGPISVSRPVYAVLSTGLEQDRTGARNNDETSPLAVESIWEVAREAGLA
                     VSAASDVPWWQQLFPRGFADYRVIPEADDYFADIPLRDLTLLHPAYIDHAGHAHGAAS
                     PEYAEAVARVDRELGTLLDRLDLTQDLVVLTADHGHSATGGHGGPTPEIARVLTCFAG
                     RGVAPRADAAPLRTHAIAGAIAVLLGLRFPRHMRAGDDDDLDHALEIPAADAFSREYL
                     ADRRAAVDHFREVNHASVAAWLGRPGHWRDLYAAERRRQWLRAAAVVLAIVAGFALAA
                     RRRRLDPRAALGLAAGFIAVLGATLLLHILVLGSLDWTAINTRERYLLRAPMICGIPA
                     VLAIAGHAWRLRDAPRLVADQLTFAALALALSLAHIVVFGWPLGFPLPGPALLLYPFL
                     GAFLVVVHGALAAVLALYVLARAALRQRSQSVQNSA"
     gene            complement(1428..2468)
                     /locus_tag="VGB85_27000"
     CDS             complement(1428..2468)
                     /locus_tag="VGB85_27000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137774.1"
                     /translation="MRWWILGGVVMLGGCLKENPAWEPDGAVTTGGLVSSSSGEALTG
                     PTSEAPTSTTGDPGTTDASGGSTNEPLTSMTEVVGSTGEHTTGDPDTTGAPAPVCKAS
                     ELVALAMPSGAPEDTGVVPSVLGSPCPWSGGQADCGPLNFGTTGFFRLVNDEALGANA
                     ALIRFPVKAVADGIFGAGKDLEDLIGARLELVIWEPKAGPAAPVDLEIHMLHPDNTDW
                     REGQRDAQPAQDDDSSALCRAIDKGDCDTWAQGDALAGATALGLLHLTPENAVSSDAD
                     SDPAQYHARLRSEPLGLALLLAYQKGEDPSLAVTLATRRDVGEGEIGIKMRESEWPDP
                     TLYAEFCTDWDR"
BASE COUNT          285 a         1082 c          863 g          384 t
ORIGIN      
        1 ggcttcgtcc tcgtcgccgt cgaggacgcc gtgcacacgc cgcccccgcg accgcggcac
       61 gccgtcgtga tcgtcgtcga cggcctcggc ctcgcccacg cccggcccct gcgatccgtc
      121 gcccggctgg ccgccgcggg ccagtgccgg gagaccgacg tcgggcctat cagcgtctcg
      181 cggcccgtct acgcggtgct gtcgaccggc ctcgagcagg accggaccgg cgcccgcaac
      241 aacgacgaga cctccccgct cgccgtcgag tcgatctggg aggtcgcccg cgaggccggc
      301 ctcgccgtct ccgctgccag cgacgtcccg tggtggcagc agctgttccc ccgcggcttc
      361 gccgactacc gcgtgatccc cgaggccgac gactacttcg ccgacatccc gctgcgcgac
      421 ctgaccctgc tgcaccccgc ctacatcgac cacgccgggc acgcccacgg ggccgcctcg
      481 ccggagtacg cggaggcggt cgcccgcgtc gaccgcgagc tcggcacctt gctcgaccgc
      541 ctcgatctca cgcaggacct cgtcgtgctg accgccgatc acgggcactc ggccaccggt
      601 ggacacggcg gaccgacccc ggagatcgcc cgcgtcctga cctgcttcgc cggccgcggc
      661 gtcgccccgc gggccgacgc cgccccgctg cgcacccacg cgatcgccgg cgcgatcgcg
      721 gtcctgctcg gcctgcgatt cccgcggcac atgcgggccg gcgacgacga cgacctcgac
      781 cacgcgctcg agatcccggc ggccgacgcc ttctctcgcg agtacctcgc ggaccgccgc
      841 gccgccgtcg accactttcg cgaggtcaac cacgcgagcg tcgccgcctg gctcgggcgc
      901 cccggacact ggcgagatct ctacgccgcc gagcgccggc gccagtggct gcgcgccgcc
      961 gcggtcgtcc tcgctatcgt cgccggcttc gcgctcgcgg cccgacgccg gcgcctcgat
     1021 cctcgcgcgg cgctcggcct cgcggccggc tttatcgccg tgctcggcgc gaccttgctg
     1081 ctgcatatac tcgtgctcgg cagcctcgac tggacggcga tcaacacccg cgagcgctac
     1141 ctgctccggg cgccgatgat ctgcgggatc ccggccgtcc tcgcgatcgc cggccacgcg
     1201 tggcgcctgc gcgacgctcc gcgcctcgtc gccgatcagc tgaccttcgc cgcgctggcg
     1261 ctcgccctct cactcgcgca catcgtcgtg ttcggctggc cgctcggctt cccgctgccc
     1321 ggcccggcgc tgctgctcta cccgttcctc ggcgccttcc tcgtcgtcgt gcacggggcg
     1381 ctcgccgccg tgctcgcgct gtacgtgctc gcgcgcgcgg ccctgcgtca gcgatcccag
     1441 tcggtgcaga actcggcgta gagggtcggg tcgggccact ccgactcgcg catcttgatc
     1501 ccgatctccc cctcgccgac gtcgcgccgg gtcgccagcg tcaccgcgag gctcgggtcc
     1561 tccccctttt gatatgcgag cagcagcgcc agcccgagcg gctcgctgcg cagccgcgcg
     1621 tgatactggg ccgggtcgct gtcggcgtcg ctgctcacgg cgttctcggg cgtcaggtgc
     1681 agcagcccca gcgccgtcgc cccggcgagc gcgtcgccct gcgcccacgt gtcacagtcc
     1741 cccttgtcga tcgcgcggca cagcgcgctc gagtcgtcgt cctgggccgg ctgcgcgtcg
     1801 cgctgcccct cgcgccagtc ggtgttgtcg gggtgcagca tgtggatctc gagatcgacc
     1861 ggcgccgcgg gcccggcctt cggctcccag atcaccagct ccaggcgcgc gccgatcagg
     1921 tcctcgaggt ccttgccggc gccgaagatc ccgtcggcca ccgccttgac cgggaagcgg
     1981 atcagcgccg cgttggcgcc gagcgcctcg tcgttgacga ggcgaaaaaa cccggtcgtg
     2041 ccgaagttga gcgggccgca gtccgcctgc ccaccgctcc atgggcacgg cgagccgagc
     2101 accgagggca ccacgccggt gtcctccggg gcccctgagg gcatcgccag cgcgaccagc
     2161 tcgctcgcct tgcacaccgg agccggcgcg ccggtcgtgt ccgggtcacc ggtcgtgtgc
     2221 tcgcccgtcg agccgacgac ctcggtcatc gacgtcaacg gctcgttcgt cgatccgccg
     2281 ctcgcgtcgg tggtgcccgg gtcgccggtc gtcgacgtcg gcgcctcgct cgtcggcccg
     2341 gtgagcgcct cgccgctgct gctgctgacc aggccgcccg tcgtcaccgc gccgtccggc
     2401 tcccacgccg ggttctcttt cagacagccg cccagcatca cgacgccccc gaggatccac
     2461 caccgcatcg ccgcagcatg ccagaagacg ccgcgcgaac gctgtatggc gggcactggc
     2521 ggggcctgtc gcgcaccacc ccgcgtcgcc cccggatcgc ctgtgccggc cgtcacagcg
     2581 ggtagcgacg gctgcgcgcc gccaccacat gcgg
//
LOCUS       DASWNJ010001625         3430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_28947, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001625 DASWNJ010000000
VERSION     DASWNJ010001625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28947"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..>3430
                     /locus_tag="VGB85_27005"
     CDS             21..>3430
                     /locus_tag="VGB85_27005"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEY0137775.1"
                     /translation="MPRTARPSPRTPCALLIALAALASCHGASKSTKAEDPALPTGVA
                     TLAAFDPAGVGPGEPPKPGPSRPLDVVDYGPQGRTEGNGELHIRFNQPVVALDLADSA
                     GLEKLFKLDPPLPGRAYWKTPDLLVYELDAAPLDCHAYTVRFTGGLVGLDGQRFDRAL
                     TWSFETPRPTVVGSDPESRPRDPDGDDDADIDNADEGQVERRDSVVLIHFDRPVALKQ
                     VQAHVQAVARPLADLTANAKPVPVRVRKATKKQRDRIYYGDDDRHVYAVQARTLWPGS
                     HEVEVSVTAGLVSEAGPLPLDTPWSMVFRTYSPQALLEVDCPPDDPCGLQPISLRLRN
                     PVSAQQIRKITLTPKPRYFSVTDFDDWGHGGRQATLTGHFVPGTTYTVHVPTGMRDIY
                     GQTIAGGATRQAVIAPRATLALSSSHGILPATGKQTIGVESRHVKALHVRAGIFTDAE
                     VEAIGLNFDASDAPFPARVLERDIPLTVTGKGDWSSLAIDLADLTGGARRPVLLEVSA
                     ADLVPRADEYGPPAPVRGLFRLTDLGPVAIVSLPASSVQVLRLSTGAPVPKARVSRFD
                     PRQRGKLLDLGLTDANGLLALPGELVPLPPVERPGEAHKPAKPLRLTVSDPTADDRAH
                     LDLIPASISRGRHEEPADEPSAVRPGERLIARFVSERGVYRPGEKVRVVGWSALDTPF
                     SRSNLGRVKTGTPVVFRLIDPTNEEVSTQATRTTSEGKFWAELAIPAQAGLGRYSVRA
                     KLLGEEIEIAVKVEDYRVPEYTVEATARSGDILAGEKATIDVHASYYFGGPVQIQRLS
                     RATRCQYQHYRPPGLEDIWVVGEPSPSHNYHGGQPRVVEPTPALATPGRRTVVDGSTL
                     EDPRYPQRCTTSLEVQDASMQGIGAEAGFSIHPAAVYLALAPPRGYLEAGQRASVPAR
                     AVDIQGKRVAADEFTLEVDRHWRERSYKPEGGQQVFAGWVERSARVKTCKPTLAATGA
                     DPACALPTLVEGRYELRATAREPGSTRVAHTFASFQVYPKSPPPDPSWRSHPVARLEV
                     LTDRSQVKPGETVAVAVRSPWPDAHGTLVVARGGIRETLPIALAGNEQLFKFTADDTW
                     TPSVAFQATIVQPSAAGSSAHPQVHDASAVVHQGQEHRRLQVVVTAPP"
BASE COUNT          504 a         1414 c         1078 g          434 t
ORIGIN      
        1 gggcatgctg tggatccccc atgcctcgca ccgcccgccc ctcgcctcgc acgccgtgcg
       61 ccctgctcat cgcactggcc gcgctcgcct cgtgccacgg cgcgagcaag tcgacgaagg
      121 cggaggaccc ggccctgccg accggcgtgg cgaccctcgc cgccttcgac cccgccggcg
      181 tcgggcccgg cgagcccccg aagccgggcc cgagccgccc gctcgacgtc gtcgattacg
      241 gcccccaggg ccgcaccgag ggcaacggcg agctccacat ccgcttcaac cagcccgtcg
      301 tcgcgctcga cctcgccgac agcgccggcc tcgaaaagct cttcaagctc gacccgccgc
      361 tacccggacg cgcctactgg aagaccccgg acctgctggt ctacgagctg gacgccgcgc
      421 cgctcgactg tcacgcctat accgtgcgct tcaccggcgg cctggtcggc ctcgacggtc
      481 aacgcttcga ccgcgcgctc acctggagct tcgagacccc ccgccccacc gtcgtcggct
      541 cggacccgga gtcgcgacca cgcgacccgg acggcgatga tgatgcggac atcgataatg
      601 ccgacgaggg tcaggtcgag cgccgcgaca gcgtcgtcct gatccacttc gatcgccccg
      661 tcgcgctcaa gcaggtccag gctcacgtcc aggccgtcgc gcgcccgctc gccgacctca
      721 ccgcgaacgc gaagccggtg cccgtgcgcg tgcgcaaggc gaccaaaaaa cagcgcgacc
      781 gcatttacta cggcgacgac gaccgccacg tctacgccgt gcaggcgcgg accctgtggc
      841 ccggcagtca cgaggtcgag gtgtcggtca ccgcgggcct cgtcagcgag gccggcccgc
      901 tgccgctcga cacaccgtgg tcgatggtat ttcgcaccta cagcccgcag gcgctgctcg
      961 aggtcgactg cccgcccgac gacccctgcg gtctccagcc gatctcactg cgcctgcgca
     1021 acccggtcag cgcgcagcag atccgcaaga tcacgctcac gccgaagccg cggtacttca
     1081 gcgtcaccga cttcgacgac tggggccacg gcggccgcca ggcgacgctc acgggccact
     1141 tcgtgcccgg caccacctac accgtccacg tgccgaccgg catgcgcgac atctacggcc
     1201 agacgatcgc cggcggcgcg acccgtcagg ccgtcatcgc cccgcgtgcc accctcgccc
     1261 tctcgtcgag tcacggcatc ctgcccgcca ccggcaagca gacgatcggc gtcgagtcgc
     1321 gccacgtgaa ggccttgcac gtgcgcgccg gcatcttcac cgacgccgag gtcgaggcga
     1381 tcggcctcaa cttcgacgcc tccgacgcgc ccttccctgc ccgtgtgctc gagcgcgaca
     1441 tcccgctgac ggtcaccggc aagggcgact ggtcctcgct cgcgatcgac ctcgccgacc
     1501 tcaccggcgg cgcccgccgc cccgtgctgc tcgaggtcag cgccgccgac ctcgtccccc
     1561 gcgccgacga gtacggcccg cccgcgccgg tccgcggcct gtttcgcctc accgacctcg
     1621 gcccggtcgc gatcgtctcg ctgccggcct cgagcgtgca ggtgctacgc ctgtcgaccg
     1681 gcgcacctgt cccgaaggcc agggtgtcgc gcttcgaccc gcgccagcgc ggcaagttgc
     1741 tcgacctcgg gctcaccgac gcgaacggcc tgctcgcgtt gccgggcgag ctcgtgccgc
     1801 taccacccgt ggagcgtccc ggcgaggccc acaagccggc gaagccgctg cgcctgacgg
     1861 tgagcgaccc gaccgccgac gaccgcgccc acctcgacct catccccgcg agcatatccc
     1921 gcggccgcca cgaggagccc gccgacgagc cctccgcggt gcgccccggc gagcgcctga
     1981 tcgcccgctt cgtcagcgag cgcggcgtct atcgcccggg cgagaaggtc cgcgtcgtcg
     2041 gctggtcggc gctcgacacc ccgttttcac gcagcaacct cgggcgcgtc aagaccggca
     2101 ccccggtcgt gtttcgcttg atcgacccta ccaacgagga agtcagcacc caggcgacgc
     2161 gcacgaccag cgagggcaag ttctgggccg agctggcgat ccccgcgcag gccgggctcg
     2221 gccgctacag cgtgcgcgcc aagctgctcg gcgaggagat cgagatcgcg gtcaaggtcg
     2281 aggactatcg cgtccccgaa tacaccgtcg aggcgactgc gcgcagcggc gacatcctcg
     2341 ccggcgagaa ggcgacgatc gacgtccacg ccagctacta cttcggcggc ccggtccaga
     2401 tccagcgcct gtcccgcgcg acccgctgcc agtatcagca ctatcgacca cctggcctcg
     2461 aggacatctg ggtcgtcggc gagccgtcgc ccagtcacaa ctaccacggc gggcagccgc
     2521 gcgtcgtcga gccgacgccc gcgctcgcca cccccggccg ccgcaccgtc gtcgacggca
     2581 gcaccctcga ggacccacgc tacccgcagc gctgcaccac ctcgctcgag gtgcaggacg
     2641 cctcgatgca gggcatcggc gccgaggccg gcttctccat ccacccggcc gccgtctacc
     2701 tcgccctcgc cccaccgcgc ggctacctcg aggccggcca gcgcgccagc gtccccgcgc
     2761 gtgccgtcga catccagggc aagcgcgtcg ccgccgacga attcaccctc gaggtcgacc
     2821 gccactggcg cgagcgcagc tacaagcccg agggcggcca gcaggtgttc gccggctggg
     2881 tcgagcgcag cgccagggtc aagacctgca agccgaccct cgccgccacc ggtgccgacc
     2941 ctgcctgcgc cctgccgacg ctcgtcgagg gccgttacga actgcgcgcg accgccaggg
     3001 agccgggctc gacccgcgtc gcgcacacct tcgccagctt ccaggtctac ccgaagtcgc
     3061 cgccgccgga ccccagctgg cgcagtcatc ccgtcgcgcg gctcgaggtg ctgaccgacc
     3121 gctcgcaggt caagccgggc gagaccgtcg cggtcgcggt gcgcagcccg tggcccgacg
     3181 cgcacggcac cctcgtcgtc gcgcgcggag ggatccgcga gacgctgccg atcgccctcg
     3241 ccggcaacga gcaactgttt aaattcaccg ccgacgacac ctggaccccc agcgtcgcgt
     3301 tccaggcgac gatcgtccag ccgtccgccg ccggcagctc cgcccatccg caggtgcacg
     3361 acgccagcgc cgtcgtgcat cagggccagg agcaccggcg cctgcaggtc gtcgtcaccg
     3421 cgccgcccaa
//
LOCUS       DASWNJ010001626         2446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_522381, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001626 DASWNJ010000000
VERSION     DASWNJ010001626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522381"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VGB85_27010"
     CDS             <1..270
                     /locus_tag="VGB85_27010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137776.1"
                     /translation="GRESIKLGSYSVEEELIRIHPVMDAADVPHFFIAWIIYHEMLHE
                     VHDMPIVDGRRVYHTPEFRRAEARFEHYAEAVLWERTQVHKLLDR"
     gene            398..>2446
                     /locus_tag="VGB85_27015"
     CDS             398..>2446
                     /locus_tag="VGB85_27015"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEY0137777.1"
                     /translation="MTTDKRSNRGKEALEFHSKGRPGKIQVVPTKPLTTQRDLSLAYS
                     PGVAEPCMEIFRDPADVYKYTARGNLVAVITDGTAVLGLGDIGPLASKPVMEGKGVLF
                     KKFADIDVFDLEVDAKNPDLFIEVVAALEPTFGGINLEDIKAPECFYIEEALKKRMNI
                     PVFHDDQHGTAIISGAGLINAAELQGKRLPELKIVVSGAGASAIACTKFYLSLGVKAE
                     NVLMADSKGILNTRRRLAEDDPKREWVRDTPADTLEEALVGADMLLGLSRAGLVTAEM
                     IKAMAPKPIIFALANPDPEIDYPTARETRPDAIVATGRSDYNNQVNNVLGFPFIFRGA
                     LDVRATQINESMKVAAAHALAELARKDVPQVVNTAYGEEFRFGPDYIIPKPFDPRVLT
                     WVAPAVAKAAMDSGVAQLTLDIDAYRERLQTLLGGSTAVMRKFMRRAAGSSRTLVLPE
                     GDDPRILKAASIIVEERIARPILLGEPDKIAKILKAHEIDLAPGSYDIVDPRTDAEAE
                     AYAAKFHRMRERKGVNLEVARLALRERNHFGMMMVAERRADGIVTGLNFAYSDTVRPA
                     LQIIGLRPGVRKIAGMYLMLHKNGMYFFGDTTVNVDMDAETLADVAEMIADAAKTFEA
                     VPRVAMLSFSNFGSVRDPRSLMVARAVELLRRRRPDLEVEGEMQANVAVDFSLQQRVF
                     P"
BASE COUNT          423 a          859 c          801 g          363 t
ORIGIN      
        1 ggccgcgagt cgatcaagct cggcagctac agcgtcgagg aggagctgat ccgcatccat
       61 ccggtgatgg acgccgcgga cgtgccgcac ttcttcatcg cgtggatcat ctatcacgag
      121 atgctgcacg aggtgcacga catgccgatc gtcgacggcc gccgcgtgta tcacacgccc
      181 gagttccggc gcgccgaggc tcgcttcgag cactacgccg aggccgtcct gtgggagcgc
      241 acgcaggtgc acaagctgct cgaccgttga cggttccgcc gccgagcgga ggtgcgcacc
      301 gcagtgcacg cctgcgcgcg acgccgctgt gcaacgtcgt ttagggccgt ctgcgacagg
      361 tgacgcggcc gcggagggcg agtatcgtgc ggccctcatg acgacggaca agcggtcgaa
      421 tcgcggcaaa gaggcgctcg agttccactc caagggccgg cccggcaaga tccaggtcgt
      481 cccgaccaag ccgctgacga cccagcgcga cctatcgctc gcctactcgc ccggcgtcgc
      541 cgagccgtgc atggagatct ttcgcgatcc cgcggatgtc tacaagtaca cggcgcgcgg
      601 caacctcgtc gccgtgatca ccgacggcac cgcggtgctc ggcctcggcg acatcggccc
      661 gctggcgagc aagccggtga tggagggcaa gggcgtgctc ttcaagaagt tcgccgacat
      721 cgacgtcttc gacctcgagg tcgacgccaa gaacccggac ctgtttatcg aggtcgtcgc
      781 cgcgctcgag ccgaccttcg gcgggatcaa cctcgaggac atcaaggcgc cggagtgttt
      841 ttatatcgag gaggcgctca agaagcgcat gaacatcccg gtgttccacg acgaccagca
      901 cggcaccgcg atcatctccg gcgccggcct gatcaacgcc gccgagctgc aaggcaagcg
      961 cttgcccgag ctcaagatcg tcgtcagtgg ggcaggggcc tccgcgatcg cttgcacgaa
     1021 gttttacctg agcctcggcg tcaaggccga gaacgtgctg atggccgaca gcaagggcat
     1081 cctcaacacc cggcggaggc tggccgagga cgaccccaag cgcgagtggg tccgcgacac
     1141 gccggccgac acgctcgagg aggcgctcgt cggcgccgac atgcttctgg gactgtcgcg
     1201 cgcgggcctc gtgaccgccg agatgatcaa ggcgatggcg ccgaagccga tcatcttcgc
     1261 gctcgccaac cccgacccgg agatcgacta cccgaccgcg cgcgagaccc ggcccgacgc
     1321 gatcgtggcg accggtcgca gcgactacaa caaccaggtc aacaacgtgc tcggcttccc
     1381 gttcatcttc cgcggcgcgc tcgacgtgcg cgcgacgcag atcaacgagt cgatgaaggt
     1441 cgcggccgcc cacgcgctcg ccgagctggc gcgcaaggac gtgccgcagg tcgtcaacac
     1501 cgcgtacggc gaggagttcc ggttcggccc cgattacatc atccccaagc ccttcgaccc
     1561 gcgcgtgctg acctgggtcg cgccggcggt ggcgaaggcg gcgatggact cgggcgtcgc
     1621 gcagctgacc ctcgacatcg acgcctaccg cgagcgcctg cagacgctgc tcggcggctc
     1681 gacggcggtg atgcgcaagt tcatgcgccg cgccgcgggc tcgtcgcgca cgctggtgct
     1741 gccggagggc gacgacccgc ggatcttgaa ggccgcgtcg atcatcgtcg aggagcggat
     1801 cgcccggccg atcttgctcg gcgagcccga caagatcgcc aagatcctca aggcccacga
     1861 gatcgacctg gcgcccggca gctacgacat cgtcgacccg cgcaccgacg ccgaggccga
     1921 ggcctacgcg gccaagtttc atcgtatgcg cgagcgcaag ggcgtcaacc tcgaggtcgc
     1981 ccgcctcgcg ctgcgcgagc gcaaccactt cggcatgatg atggtcgccg agcgccgcgc
     2041 cgacggcatc gtcaccggcc tcaacttcgc gtactccgac acggtccgcc cggccctgca
     2101 gatcattggt ttacgccccg gcgtgcgcaa gatcgccggc atgtacctga tgctgcacaa
     2161 aaacggcatg tacttcttcg gcgacacgac cgtgaacgtc gacatggacg ccgagacgct
     2221 cgccgacgtc gccgagatga tcgccgacgc cgccaagacc ttcgaggcgg tcccccgcgt
     2281 cgccatgctc agctttagca acttcgggtc ggtccgtgac ccccggtcgt tgatggtcgc
     2341 acgcgcggtg gagctcctgc gccggcgccg gcccgacctc gaggtcgagg gtgagatgca
     2401 ggccaacgtc gccgtcgact tcagcctgca acagcgcgtg ttcccg
//
LOCUS       DASWNJ010001627         6085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_624886, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001627 DASWNJ010000000
VERSION     DASWNJ010001627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6085
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624886"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VGB85_27020"
     CDS             complement(<1..204)
                     /locus_tag="VGB85_27020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137778.1"
                     /translation="MVSGAIVVELPPLLSPLLSPLLSEPPVVVSPVVVPLLSEPVDVG
                     TTVLLESAVAEPSPVVFPVLPVDG"
     gene            complement(350..1621)
                     /locus_tag="VGB85_27025"
     CDS             complement(350..1621)
                     /locus_tag="VGB85_27025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0137779.1"
                     /translation="MTVRALRTTDLDAPRPIYVVWETTLRCDHECAHCGSRAGDARPS
                     ELSTDELLAVADSLIGLGAREVTLIGGEAYLRGDCYRLISHLRDGGIRVTMQTGGRGL
                     TPDRARKLKAAGLAAIGVSVDGPPAAHDELRASPGSHAAALAGIANARAAGIVVTSNT
                     QVNRRNKDHLRETAALLKDAGVAVWRAQLTAPMGRAADEPDWILQPYMILEVIDTLAE
                     IQHAAIAEAEARGVSPDHAFHVRLGNNLGYYGPHEQLLRARPGAQGGHWQSCGAGKFV
                     MGIESDGTIKGCPSLPTGPYAGGNVRELSLDQIWEHSEVIAFARNRDESELWGFCKTC
                     YYAEVCRAGCSFTSHTTLGRRGNMPFCYYRASLLRRQGLRERLLHKTQAAGLPYDFGA
                     FELAVEPWPSTPDPPERPRTRLPILAAGPTV"
     gene            1821..2939
                     /locus_tag="VGB85_27030"
     CDS             1821..2939
                     /locus_tag="VGB85_27030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137780.1"
                     /translation="MRSAVTAVWWCGALWTAGCFTGGFLAGQPCAGDDDCGPSLRCED
                     GVCGGPQASASTGSSSSAGTTVPTMADPTSTGADTSTSTGTATPSTGEDTSSGAASST
                     TTGMDRCADATCGPLDLLLIIDDSPSMAQWQPELSAALQSLDAGPVGALIRGSCDAHI
                     GVLATGAVYDANPQPCQGLGSLVRIGKQPCAGVAPYATQTDDLTSALDCRISVGSDGP
                     ADERPVQALLQAMTPEYNDAGACNDGFFRADSLLVVVFVTDEDDDADAEDEAPGEQTP
                     GTPQAWFDTVVAFKGSAERVVMLALTGESGPVTLCPWTPGPGDADGMGAEAPDRLHSF
                     IDLFPRRAVDTLCQRDYVGFVDTVYTQLHDACMAAATP"
     gene            complement(2936..3784)
                     /locus_tag="VGB85_27035"
     CDS             complement(2936..3784)
                     /locus_tag="VGB85_27035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137781.1"
                     /translation="MLVITLIMVARPALAAPATPQTREPAGVPLAVSGDGIEGIDDPM
                     SRVASLINEGQALFDTADFIGAIDRWTKAYASLPDAPDIHTARNLLTYQIAQAHIEAH
                     AIDGQPTHLRKAERLLSRYIEGLAPEEAEARASADELRDDLRQRIATAPPPVVIAEAP
                     PPPPPQLDVPRRRSRLMLAGGITLGAGGALLVGTLVAALEGQHIDRDGERAVMRGAED
                     AELHELLSRGTRANQAAIGTAVAGALLVTTGVALLITGRMRRGPLALAPSFGPGLVGM
                     SFRTRF"
     gene            3940..5502
                     /locus_tag="VGB85_27040"
     CDS             3940..5502
                     /locus_tag="VGB85_27040"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0137782.1"
                     /translation="MNASVENTGEACLTAGESGATRVTSSGPPPSPAQIPTAELMALM
                     SDRYRILRRIGRGGMGEVFLAEHAAIGKQVAIKILSEDFARKPRIVQRFLQEARAASL
                     IRHENVVDITDFGHTPSGLPFLTMEYLDGEDLKAMLRREIRLPWPRARRILLQVLAAL
                     EAAHAQGVIHRDIKPDNCFRLTRGENQDFIKVLDFGIAKVLGDEIDQALTQTGVVMGS
                     AEYMSPEQAKSEPIDARTDLYSVGVILYQMLTGKVPFRANGFMGTLSKHIMEPPPPPR
                     QIAPEAKITRPLEQLVLKALAKEPGDRFQSAAEFAAALRALPPGRDEPIVKRYTTWWT
                     AGLGVALCAGGLMAWGLLPGPATPVNDGVAEQMLAVPDEPQPNAAPLPPVLAPLDPGA
                     QGPSQPDGPGEPGTADPGTNEPGTGSPVEPGKRPQRPRPKLPEVLSVAQLREGFAQVE
                     SLARECRKYGVPGAVVHVQLQVTPEGRVVGAAARKPYQGTSLGSCVEAAASKARFPPA
                     RTGQRAEPGFKL"
     gene            complement(5512..>6085)
                     /locus_tag="VGB85_27045"
     CDS             complement(5512..>6085)
                     /locus_tag="VGB85_27045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137783.1"
                     /translation="TVATSTTTVGTSDTNDTSDTDFPPPVDPPPPCEGEAMQLAGITT
                     KVGYLQSQVPPRQDPTGGTTSTTGGGDLDPGTLFVKLSDQAFSCADPNASLQCGEHWE
                     VTIVIPPEFQSPGVHNLLGQNVRGIATETGVDEGMDSCAFGGGSFGATFEILAIDDKT
                     VEGRLCNVESFFSFANPQLEGSFTATLCPQ"
BASE COUNT          851 a         2059 c         2382 g          793 t
ORIGIN      
        1 cccgtcgacc gggagcaccg ggaacaccac cggcgacggc tcggcgaccg ccgactcgag
       61 cagcacggtg gtgccgacgt cgaccggctc ggagagcagc ggcacgacga ccggcgacac
      121 gacgaccggc ggctcggaga gcagcgggga gagcagcggg gacagcagcg gcggcagctc
      181 gacgacgatg gcgccggaga ccaccggcga gccggactac ggggtgccgt cgacgacgac
      241 cgggcccgag ccggactacg gcgtgcccgg cacgacctga ccccggggac aggctgtcct
      301 cgcggacagc agcaatcgtg agacctgtcc tcgcggacag gtccgatatc tacacggtcg
      361 gcccggcggc gaggatcggc aggcgggtgc gcgggcgctc gggcgggtcg ggggtgctcg
      421 gccacggctc gacggcgagc tcgaaggcgc cgaagtcgta ggggaggccg gcggcctggg
      481 tcttgtgcag caggcgctcg cgcaggccct ggcggcgcag caggctggcg cggtagtagc
      541 aaaacggcat gttgccgcgg cggccgagcg tcgtgtgcga ggtgaacgag cagccggcgc
      601 ggcagacctc ggcgtagtag caggtcttgc agaagcccca cagctcgctc tcgtcgcggt
      661 tgcgggcgaa ggcgatcacc tcgctgtgct cccagatctg gtcgagcgag agctcgcgga
      721 cgttgccgcc ggcgtagggt ccggtcggca gcgaggggca gcccttgatc gtgccgtcgg
      781 actcgatgcc catcacgaac ttgccggcgc cgcagctctg ccagtggccg ccctgggcgc
      841 ccggacgggc gcgcaggagc tgctcgtgcg ggccgtagta gccgaggttg ttgccgaggc
      901 ggacgtgaaa ggcgtggtcg ggcgagacgc cgcgcgcctc ggcctcggcg atcgcggcgt
      961 gctggatctc cgcgagcgtg tcgatcacct cgaggatcat gtagggctgg aggatccagt
     1021 ccggctcgtc ggcggcgcgg cccatcggcg cggtcaactg ggcccgccac acggcgacgc
     1081 cggcgtcctt gagcagcgcg gcggtctcgc gcaggtggtc cttgttgcgg cggttgacct
     1141 gcgtattgct ggtgacgacg atgccggccg cgcgcgcgtt ggcgatgccc gcgagcgcgg
     1201 cggcgtggct gccggggctg gcgcgcagct cgtcgtgggc ggccggcggc ccgtcgacgg
     1261 acacgccgat cgcggcgagg ccggcggcct tgagcttgcg cgcgcggtcg ggggtcagtc
     1321 cgcgtccgcc ggtctgcatc gtgacgcgga tcccgccgtc gcgcaggtgg gagatcaggc
     1381 ggtagcagtc gccgcgcagg taggcctcgc cgccgatcaa ggtgacctcg cgggcgccga
     1441 ggccgatcag gctgtcggcg accgcgagca gctcgtcggt gctcagctcg gacggtcggg
     1501 cgtcgccggc acgcgagccg caatgggcgc actcgtggtc gcagcgcagg gtcgtctccc
     1561 acacgacgta gatcgggcgg ggcgcgtcga ggtccgtggt acgcagcgcg cggacggtca
     1621 tgcgggcagt gtatcggcgg ggccggaaaa aggggccgtg aactcgcgga cgtcggcgcg
     1681 ctcccccggc cgcgggccgc ggcgccggcc ggcccgaacg cgccgtggcg gggtcgcagg
     1741 ggcgctcccg ggcgtgctgc ggggcgcacc ggctcgccgc gccgccgctc gtgcacgcgc
     1801 gcccgtgcta gcttgcgggg gtgcggtctg cagtgacggc ggtgtggtgg tgcggggcgc
     1861 tgtggaccgc ggggtgcttc acgggcggct tcctggcggg ccagccctgc gccggcgacg
     1921 acgactgcgg gccgagcctg cgctgcgagg acggcgtgtg cggaggcccg caggcgagcg
     1981 cgagcacggg cagcagcagc agcgccggga cgacggtgcc gacgatggcc gacccgacga
     2041 gcacgggcgc ggacacgagc acgagcaccg gcacggcgac gccgagcacc ggcgaggaca
     2101 cctcgtcggg cgccgcgagc tcgacgacca cggggatgga ccgctgcgcc gacgcgacgt
     2161 gtggtccgct cgacctgctg ctgatcatcg acgactcgcc gagcatggcg cagtggcagc
     2221 cggagctgtc ggcggcgctg cagagcctcg acgccggccc ggtcggtgcg ctgatccgcg
     2281 gcagctgcga cgcgcacatc ggggtgctgg cgaccggcgc cgtgtacgac gccaacccgc
     2341 agccgtgcca gggcctcggc tcgctggtcc ggatcggcaa gcagccgtgc gccggggtgg
     2401 cgccctacgc gacgcagacc gacgacctga cgagcgcgct cgactgccgg atcagcgtcg
     2461 gcagcgacgg cccggcggac gagcggccgg tccaggcgct gctgcaggcg atgacgccgg
     2521 agtacaacga cgccggggcc tgcaacgacg gcttctttcg cgccgactcg ctgctggtgg
     2581 tcgtgttcgt caccgacgag gacgacgacg ccgacgccga ggacgaggcg cccggcgagc
     2641 agacgcccgg cacgccgcag gcgtggttcg acacggtcgt cgcgttcaag ggctcggcgg
     2701 agcgcgtcgt catgctcgcg ctgaccggcg agtccgggcc cgtcacgctg tgcccgtgga
     2761 cgccggggcc cggcgacgcc gacggcatgg gcgccgaggc gcccgaccgc ctgcacagct
     2821 tcatcgacct gttcccgcgc cgcgccgtcg acacgctgtg ccagcgcgac tacgtcggct
     2881 tcgtcgacac cgtgtacacg cagctgcacg acgcgtgcat ggccgcggcg acgccctaga
     2941 aacgtgtccg aaaggacatg cccacgaggc caggtccaaa gctcggcgcg agcgcgagcg
     3001 gaccgcgacg catgcggccg gtgatcagca gggcgacgcc ggtggtgacg agcagggcgc
     3061 cggccaccgc ggtgccgatc gcggcctggt tggcccgggt gccgcgcgac agcagctcgt
     3121 gcagctcggc gtcctcggcc ccgcgcatca ccgcacgctc gccgtcgcgg tcgatgtgct
     3181 ggccctcgag cgcggcgacg agggtgccga cgagcagggc cccgccggcc ccgagcgtga
     3241 tcccgccggc gagcatcagg cggctgcgac ggcgcgggac gtcgagctgc gggggcggag
     3301 gcgggggcgc ctcggcgatc acgaccggcg gcggcgccgt ggcgatccgc tggcggagat
     3361 cgtcacggag ctcgtcggcg gaggcgcggg cctcggcctc ctcgggcgcg agcccctcga
     3421 tgtaacgcga gagcagacgc tctgccttgc gcaggtgcgt cggctggccg tcgatcgcgt
     3481 gcgcctcgat gtgggcctgt gcgatctggt aggtcaggag gttgcgcgcc gtgtggatgt
     3541 ccggcgcgtc gggcagcgat gcataggcct tggtccagcg atcgatcgcg ccgatgaagt
     3601 ccgcggtgtc gaacagcgcc tgtccctcgt tgatcagcga ggcgacgcgg gacatcgggt
     3661 catcgatacc ctcgatacca tcaccgctga cggcgagcgg gacgccggca ggttcacgag
     3721 tttgtggggt tgcaggagcc gccagcgcag gccgcgcgac catgatcaac gtgatcacca
     3781 gcatcgccgc cacccatcgc tgtgctccga ccccgaccct cacggggagc cagcatggcg
     3841 ggggtcggtc gggcgagcaa cccgccggaa ggtatgccgg tgtgcgtcgc gcgatcatga
     3901 agaggggcca cggacgcggt ccggcgttat cctggcgccg tgaatgcctc ggtggaaaac
     3961 acgggcgagg cctgcctgac ggcgggggag tccggtgcca cgcgggtcac cagctccggt
     4021 ccgccgccat cccccgccca gatcccgacg gcggagctga tggcgctgat gtccgaccgt
     4081 taccggatcc tccgcaggat cggtcgcggc ggcatgggtg aggtcttcct ggccgagcac
     4141 gcggcgatcg ggaagcaggt ggcgatcaag atcctcagcg aggacttcgc ccgcaagccg
     4201 cgcatcgtgc aacgcttcct gcaagaggcc cgcgcggcgt cgttaatccg ccacgagaat
     4261 gtcgtcgaca tcacagactt cgggcacacg ccgagcgggc tcccgtttct tacaatggag
     4321 tacctcgacg gcgaggatct caaggcgatg ctgcggcgag agatccgcct gccgtggccg
     4381 cgggcgcgac ggatcttgct gcaggtgctc gcggcgctgg aggccgcgca cgcgcagggc
     4441 gtcatccacc gcgacatcaa gcccgacaat tgctttcgcc tgacccgcgg cgagaaccag
     4501 gacttcatca aggtcctcga cttcgggatc gccaaggtgc tcggcgacga gatcgatcag
     4561 gcgctgacgc agaccggcgt ggtgatgggc agcgccgagt acatgtcgcc cgagcaggcc
     4621 aagagcgagc cgatcgacgc gcgcaccgac ctctactcgg tcggcgtgat cctctatcag
     4681 atgctgaccg gcaaggtgcc gtttcgggcc aacggcttca tgggcacgct gagcaagcac
     4741 atcatggagc cgccgccgcc tccgcggcag atcgcaccgg aggcgaagat cacccggccg
     4801 ctcgagcagc tggtgctcaa ggcgctggcg aaggagccgg gcgaccgctt tcagagcgcg
     4861 gccgaattcg cggcggccct gcgcgcgctg ccgccggggc gcgacgagcc gatcgtcaag
     4921 cgctacacga cgtggtggac ggcgggcctc ggggtggcgc tgtgcgccgg cgggctgatg
     4981 gcgtggggcc tgctgcccgg gcccgcgacg ccggtcaacg acggcgtcgc cgagcagatg
     5041 ctggcggtcc cggacgagcc gcagcccaac gcggcgccgc tgccccccgt gctcgcgccg
     5101 ctcgaccccg gcgcgcaggg gccgagccag ccggacgggc ccggtgagcc ggggaccgcc
     5161 gacccgggga cgaacgagcc gggcacgggg tcgccggtcg agccgggcaa gcgaccgcag
     5221 cggccgcggc cgaagctgcc ggaggtgctc agcgtcgcgc agctgcgcga gggcttcgcg
     5281 caggtcgagt cgctcgcccg cgagtgtcgc aagtacgggg tgcccggcgc ggtcgtgcac
     5341 gtgcaactgc aagtgacgcc cgagggtcgg gtcgtcggcg ccgcggcgcg caagccgtat
     5401 cagggaacct cgctcggcag ctgcgtcgag gcggccgcga gcaaggcccg gttcccaccg
     5461 gcgcgcacgg gtcagcgcgc cgagccgggc ttcaagctgt gagccagcag ctcactgcgg
     5521 gcacagggtg gcggtgaagc tgccctcgag ctgcgggttg gcgaagctga agaagctctc
     5581 gacgttgcac aggcggccct cgacggtctt gtcgtcgatg gcgaggatct cgaaggtggc
     5641 gccgaagctg ccgcccccga aggcgcagga gtccatgccc tcgtcgacgc cggtctcggt
     5701 ggcgatgccg cgcacgttct gaccgagcag gttgtgcacg ccggggctct ggaactcggg
     5761 cgggatcacg atcgtgacct cccagtgctc gccgcactgg agcgacgcgt tggggtcggc
     5821 gcagctgaag gcctggtccg agagcttgac gaacagggtc ccgggatcga ggtcgccgcc
     5881 gccggtcgtg ctggtggtgc cgccggtcgg atcctgccgc ggcgggacct gggactgaag
     5941 gtagccgacc ttggtggtga tgcccgccag ttgcatcgcc tcgccctcgc aggggggcgg
     6001 cgggtcgacc gggggcggga agtcggtgtc gctggtgtcg ttggtgtcgc tggtgccgac
     6061 ggtcgtcgtg ctggtggcga cggtg
//
LOCUS       DASWNJ010001628         2577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_168673, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001628 DASWNJ010000000
VERSION     DASWNJ010001628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168673"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1976
                     /locus_tag="VGB85_27050"
     CDS             <1..1976
                     /locus_tag="VGB85_27050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137784.1"
                     /translation="KKKYEPAFKRMREDMRFARGLQYPGQTSIDDDRASVDIIQRHIN
                     ARVASLYAKNPTFAAKRRKRMDFALWDGDGATLQRTAQTIAEAMAAGAMPDPNTVALM
                     TDIQEGITRRRMLDRIGRTLEIVMAYSLDEQQPSFKARCKRLVRRTVTCGVGYLKVGY
                     QRALQARPDAAAELGDITTRIATQERLSADLADGISDDQSADAEALRVTQEGLAAEQD
                     VLVREGLVFDFPAATRIIPDVNTTELVGWVGSQWVTEELLMSAEEIKEVYSVDVGDKF
                     TGYRASVTGDATVRTAHNAKDRKALVWVMHHKKHGVVYHMVDGYPDFLRDPAPPPVQI
                     EQFFPFFPLLFNDMEAEDELFPRSDVRLLRSPQREMNRKREAVRQHRIAARPRYLGQK
                     GKFEDGELTDISTLADHEVLFVNALEAGEKLENVIQKLPAANIDPNLYETGSDMNDVQ
                     LIVGASESQLGNPGGKTTATGDAIAEQSRLASVTSNADDLDDLLSAVAGAAGDILLSE
                     MDRDTVLKIAGPGGVWPELDRQSIAEDLFLEIEAGSSGRPNKAQDIANFERMAPTLMQ
                     IPGISPEWLAQHAIKILDASIDFEDAITAGLPSIVQQNSAPQPSTGDPATDPAQQGGE
                     GGNNAPNNDTSAGGGQPAMPADGGQAAPESAPV"
     gene            2023..>2577
                     /locus_tag="VGB85_27055"
     CDS             2023..>2577
                     /locus_tag="VGB85_27055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137785.1"
                     /translation="MSVQDTSASPVEEIENAEATGSPEVEGSDATAGSSPQDANASPP
                     SLLDVVKSAVAPAEPDAEGTSSPEAEKEGQAADDTEANAAEDVDDEKVNPGLHKHPRF
                     RAVLTERNTLREQVTALKEPAQQYERIEAFMQHNGLTNEHVVELFRVGALMRTDPDKA
                     YEAVADTLVELGKLTGRSLPEDLRE"
BASE COUNT          506 a          893 c          829 g          349 t
ORIGIN      
        1 ccaagaaaaa gtacgagccc gccttcaagc ggatgcgcga ggacatgcgg ttcgcgcgcg
       61 gcctccaata ccccggccag acgtccatcg acgacgaccg cgcctcggtc gatatcatcc
      121 agcggcacat caacgcccgc gtcgcctcgc tctacgccaa gaacccgacg tttgccgcga
      181 agcgccgcaa gcgcatggac ttcgccctgt gggacggcga cggcgcgacc ctccagcgca
      241 ccgcgcagac catcgccgag gctatggccg ccggggcgat gcccgatccc aacaccgtcg
      301 ccctgatgac cgacattcag gaggggatca cgcgccgccg gatgctcgac cggatcggcc
      361 gcaccctcga aatcgtcatg gcctactcgc tcgacgagca gcagccgtcg ttcaaggccc
      421 ggtgcaagcg cctcgtgcgc cggaccgtca cctgcggcgt cggctacctc aaggtcggct
      481 atcagcgggc gttgcaggcg cggccggacg cggcggccga gctgggcgat atcaccacgc
      541 ggatcgcgac gcaggagcgc ctgtcggccg acctcgccga tgggatcagc gacgatcaga
      601 gcgccgacgc cgaggccctg cgcgtgacgc aggaggggtt ggccgccgag caggacgtcc
      661 tcgtgcgcga gggcctcgtg ttcgacttcc cggccgcgac ccggatcatc cccgacgtga
      721 acacgacgga gctggtcggc tgggtaggct ctcagtgggt gactgaggag ctgctcatgt
      781 cggccgagga aatcaaagag gtctactcgg tcgatgttgg cgacaagttc accggctacc
      841 gcgccagcgt gaccggcgac gccaccgtgc ggaccgccca caacgccaag gatcgcaagg
      901 cgctcgtgtg ggtcatgcac cacaagaaac acggcgtcgt ctatcacatg gtggacggct
      961 accccgactt cctgcgcgac ccggccccgc cgcccgtcca gattgagcag ttcttcccgt
     1021 tcttcccgct gctgttcaac gacatggagg ccgaggacga gctgttcccg cgctccgacg
     1081 tccggctgct gcgctcgccc cagcgcgaga tgaaccgcaa gcgcgaagcc gtccggcagc
     1141 accggatcgc ggcgcgcccc cgctacctcg gccagaaggg caagtttgag gacggcgagc
     1201 tgaccgatat ctcgacgctc gccgaccacg aggtcctgtt cgtcaatgcg ctggaggcgg
     1261 gcgagaagct ggagaacgtg atccagaagc tcccggccgc caacatcgac ccgaacctct
     1321 acgagacggg ctcggacatg aacgacgtcc agctgatcgt cggcgcgtcc gagagccagc
     1381 tgggcaaccc cggcggcaag acgaccgcga ccggcgacgc catcgccgag cagtcccgtc
     1441 tggcctcggt cacgtccaac gccgacgacc tcgacgacct gctctcggcg gtcgcgggcg
     1501 cggcgggcga tatcctgctg tcggagatgg atcgcgacac ggtcctgaaa atcgcgggcc
     1561 ccggcggcgt ctggcccgag ctggaccggc agtccatcgc cgaggacctg ttcctcgaaa
     1621 tcgaagcggg ctccagcggt cggccgaaca aggcccagga catcgcaaac tttgagcgga
     1681 tggccccgac gctcatgcaa atccccggca tctcgcccga gtggttggct cagcacgcca
     1741 tcaagatttt ggacgccagc atcgactttg aggacgccat cacggcgggc ctcccgagca
     1801 tcgttcagca gaacagcgcg ccgcaaccgt ctacgggcga cccggcgact gatcctgccc
     1861 agcagggcgg ggaagggggc aataacgccc cgaacaacga cacgagcgca gggggtggtc
     1921 aaccggccat gccagcggac gggggacaag ccgcccctga aagcgcgcct gtgtagttga
     1981 cagcgacgtt caaatacgtt caacagaggg aaggcgctaa atatgtctgt tcaggacacc
     2041 tcggcatcgc cggtcgagga aatcgaaaat gccgaagcca cgggttcgcc cgaggtcgaa
     2101 gggtcagacg ctacggcggg ttcgtctccg caggacgcta atgcaagtcc gcccagcctg
     2161 ctcgacgtcg tcaaaagcgc cgtcgctcct gctgaaccgg atgccgaggg cacgtcctcc
     2221 ccggaagccg agaaggaagg ccaagccgcc gacgacaccg aggccaatgc ggccgaggac
     2281 gttgacgacg agaaggtcaa cccaggactc cacaagcacc cccggttccg cgcggtcctg
     2341 accgaacgga acacgctcag ggagcaggtg acggcgctca aggaaccggc ccagcagtac
     2401 gagcgcatcg aagcgttcat gcagcacaac gggctcacca acgagcatgt cgtcgagctg
     2461 ttccgagtcg gcgcgctcat gcgaaccgac cccgacaaag cctacgaggc cgtcgctgac
     2521 acgctggttg agctgggcaa actcaccggc cgatccctgc ccgaggacct ccgcgag
//
LOCUS       DASWNJ010001629         7518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_488396, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001629 DASWNJ010000000
VERSION     DASWNJ010001629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7518
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488396"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="VGB85_27060"
     CDS             <1..1071
                     /locus_tag="VGB85_27060"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0137786.1"
                     /translation="TLSSRAGIRVGTEFIASSYDLSASVATASAGPGGGGGSNPGQQS
                     STNSATLIRPALYVTIPLKLGERVTLYPGARLTYYGAPFNRAMVEPRLRMTWDVADRT
                     TLKAGLGLYAQTPQPREFDAVFGNPRVGLEHALHTSLGVAQILPQDITLEVTGFYKNL
                     WGLARGSSGLQLVDGLLRPENLASTGVGYVVGGELLLRKALTRKLFAWLSYTLMRSVR
                     REAAGEPAYLFGFDQTHIMTAIASYKLPRNWQIGARFRLISGNPYTPVLGGVFDAGDG
                     GFISVSGPRNSARLPAFHQLDLRIDKRWLWRRISLNAYIDVLNIYNHVNTERLQNSYD
                     FQTTAPIASLPILPSVGLRLEW"
     gene            1119..2291
                     /locus_tag="VGB85_27065"
     CDS             1119..2291
                     /locus_tag="VGB85_27065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137787.1"
                     /translation="MPLRRLALRLRHLVFGTGAMLAACGGDLPIAERIASTRVLAIRN
                     EVITPLYPDPGPDLGVRCEALPFEQVRLTPWVVTPEGPLDLTGPDFDPIYIACALGPG
                     QGLFACLKAAVPTTLADIPECPVPSFADLDPMAMGLPDPPSPCILPADDSDDGAQDFT
                     VPFASALLIGGDLEVTMISQISRSPGSPDTEACAAALLGNQSDLPNECLYAVQRVPVG
                     PIERLLLLAAEFGVDLPPELGAPPDPAAVPDGDRNPRILDFSVTLVRDGESTDLGPKN
                     RGDVVQARLGDVLKIATNAPPTDLQEFLVPVNGGAGGTEIQAESYDGSWYRSWGTLQA
                     GGSDDPLSENEWRMEMGTQDEDLSPPDGRATLFYVLRDGRVGLDWWWISVEVEPAS"
     gene            2439..4364
                     /locus_tag="VGB85_27070"
     CDS             2439..4364
                     /locus_tag="VGB85_27070"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY0137788.1"
                     /translation="MSLSLAMLLAGLTVSQNPTVNPETGLARDVQQRIEPPVVTPRPS
                     PDVQSPSSTASPGMRPPSTTDPGMGTSTAPGRTPLRRPGTEPMPSSPPTTGTPAPVPG
                     QQTPRPTSPGELYPLSFATESLELSQVLKTALENNLDLRSTAFDVAISEKNIMVALGA
                     YDVFMTGSLYAAKAVTPQRGSQLAFNLGQTQVGGSVGFERKLETGGTVNLTVGASRTL
                     FLQPLQIGNAAAGTVNLGSYIVAPTLTLTHPLLRNAGLRVNRADIDRARLATTRAEAG
                     EMLSAQTIVRDLILAYWDLLFASRDLDNKRRSASTTAEQHRRTQAEVAAGRKSQLDLD
                     TIYQSLVARENDVVLAENVLLDRSLTVRTLMGQDFVDRKVLGIIPQTDPQAIALTPVD
                     LNAKVKQALKGHPQLRQLEISLASQRIDELVAANKRLPQLDARVVFAPQGRSIDTRAQ
                     PAAGTPALSGNWGQAFQNFFNTGDQASTTSGLLADYSARAELTFSWDIQNRAPKATHE
                     RIILEMRRAEAQLRRNRQNIAMSVIRAVNAQRTAGARMTITSESVRLAQSNLRAEEAR
                     NRVGRSTSYDVLFRQDELAIAEFNALNAQIDYLRATVDLQMLTGELLPSYGLELASRS
                     ARISQDDQANADPGRYE"
     gene            4460..4672
                     /locus_tag="VGB85_27075"
     CDS             4460..4672
                     /locus_tag="VGB85_27075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137789.1"
                     /translation="MSEIADNKWEAEAAKPPVSTVSGIAQILLIACFAGVFVYILQGS
                     ARGLFSEKKDGLAENYGKMTNEAGKE"
     gene            complement(4785..5510)
                     /locus_tag="VGB85_27080"
     CDS             complement(4785..5510)
                     /locus_tag="VGB85_27080"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HEY0137790.1"
                     /translation="MIDRVPAFGGCVHGFTDRLGGVSEGRHASLNLGRRWGDDPAAVA
                     ENYRRVAAAAGFRVEDLRQVRQVHGKTVLRARDVTAASEADALWVHREDGPLVAGVLT
                     ADCVPILLTDVDGTVAAAVHSGWKGTVADIAGATVGVLAEHVAPARLLAAIGPCIEVE
                     AFEVGEEVAVQFPAEHVRREGTRKPHVDLVATVRAQLIAAGVPAGAISRVGGCTFSDP
                     TRYFSYRRDGAGIGQMLAFIGLG"
     gene            complement(5507..6484)
                     /locus_tag="VGB85_27085"
     CDS             complement(5507..6484)
                     /locus_tag="VGB85_27085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY0137791.1"
                     /translation="MSRLRVSLGPEALGQRLDAALVAALQRAGHNCSRSQLARAFAAG
                     GVKVAGAVAGKPSRVVEAAVAVELELEIIKPVQAVAEDLPLTIVHEDEHLLVIDKPAG
                     MVVHAGPGHDRGTLVNAVLHHLGISAKDLPVLPGNDETRPGIVHRLDRDTSGLIVVAR
                     NERAQEGLAAQFRVHDLRRRYLGIVWGVPGFVKKSLRTRHGRDPGDRRRFSPHIDEGR
                     EAITHLEVLRPLRDAALLAFRLETGRTHQIRMHCRLLGHPIVADAMYGGNGGPPARRA
                     ISAALGRHALHAAELGFVHPISGAALDFSSPLPPDLVQLVEQLSPERAA"
     gene            complement(6481..6750)
                     /locus_tag="VGB85_27090"
     CDS             complement(6481..6750)
                     /locus_tag="VGB85_27090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137792.1"
                     /translation="MAATPIDNDARAQQLARAIASDIQLYNKQKIEEALINDNFFEAL
                     AEEITEGRDLFRGRVTPELFRRNYYDRAIVDRVVKPMASLKTPIW"
     gene            6896..>7518
                     /locus_tag="VGB85_27095"
     CDS             6896..>7518
                     /locus_tag="VGB85_27095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137793.1"
                     /translation="MSGSNLYASLSSLLAAAVAIAIGISVFLRDRSRDQFRLFAACCL
                     NLGLFHLARFFAGFPGLPLSIWVAQTVSLFLPYTADRCFSGFIPASGAPPRNLVQAPI
                     LALLLIAQALSLLFPGLSDDPAWGIVPFLVQAYVLGGLLYAAMRMWRAARAAEGTAAA
                     PRLRYLFYASVLALALGQPGIPVVGATVTAVYLYFMAQTLVRERLLDL"
BASE COUNT         1095 a         2994 c         2342 g         1087 t
ORIGIN      
        1 accctcagca gccgcgccgg catccgcgtc ggcaccgagt tcatcgcctc gagctacgac
       61 ctctccgcgt ccgtcgccac cgccagcgcc ggtcctggcg gcggcggcgg ctcgaacccg
      121 ggccagcagt cctcgaccaa cagcgccacg ctgatccgcc cggccctcta cgtcacgatc
      181 cccctcaagc tcggcgagcg cgtcacgctc taccccggcg cccggctcac ctactacggc
      241 gcgcccttca accgcgccat ggtcgagccg cgcctgcgca tgacctggga cgtcgccgac
      301 cgcaccaccc tcaaggcggg cctcggtctc tacgcccaga ccccgcagcc gcgcgagttc
      361 gacgccgtgt tcggcaaccc gcgcgtcggc ctcgagcacg cgctgcacac cagcctcggc
      421 gtcgcgcaga tcctcccgca ggacatcacc ctcgaggtca ccggcttcta taaaaacctg
      481 tggggcctcg cgcgcggctc gtctggcctc cagctcgtcg acggcctcct gcgcccggag
      541 aacctcgcca gcaccggcgt cggctacgtc gtcggcggcg agctgctgct gcgcaaggcg
      601 ctgacccgca agctgttcgc ctggctcagc tacaccctga tgcgcagcgt ccggcgcgag
      661 gccgccggcg agccggccta cctcttcggc ttcgaccaga cccacatcat gaccgcgatc
      721 gcgagctaca agctcccgcg caactggcag atcggcgcgc gcttccgcct gatctccggc
      781 aacccgtaca cgcccgtgct cggcggggtg ttcgacgccg gcgacggcgg cttcatctcg
      841 gtgtccggcc cgcgcaacag cgccaggctg cccgcctttc accagctcga cctgcgcatc
      901 gacaagcggt ggttgtggcg ccgcatcagc ctcaacgcct acatcgacgt gctcaacatc
      961 tacaatcacg tcaacaccga gcgcctgcag aacagctacg acttccagac caccgccccg
     1021 atcgcctccc tcccgatcct gccctccgtc ggcctgcgcc tcgaatggtg aagatccccc
     1081 actggtgcgc cggcccatgg tcgcgatatc ctcgcgccgt gcccctccgt cgcctcgcgc
     1141 tgcgcctgcg ccacctggtc ttcgggacag gtgcgatgct cgcggcctgc ggcggcgacc
     1201 tgccgatcgc cgagcggatc gccagcaccc gcgtcctggc gatccgcaac gaggtgatca
     1261 ccccgctcta ccccgacccc gggcctgacc tcggcgtgcg ctgcgaggcg ctgcccttcg
     1321 agcaggtgcg cctgaccccg tgggtcgtca cgcccgaggg cccgctcgac ctcaccggcc
     1381 ccgacttcga cccgatctac atcgcctgcg ctctcggccc cggccagggc ctgttcgctt
     1441 gcctcaaggc cgcggtcccg accaccctcg ccgacatccc ggagtgtccg gtcccctcgt
     1501 tcgccgatct cgacccgatg gccatgggcc tgccggaccc gccgagcccc tgcattctcc
     1561 cggcggacga cagtgacgac ggcgcccagg acttcaccgt ccccttcgcc tcggccctgc
     1621 tgatcggcgg tgacctcgag gtcacgatga tcagccagat cagccgcagc cccgggtcgc
     1681 ccgacaccga ggcctgcgcc gccgcactgc tcggtaacca aagcgacctg ccgaacgagt
     1741 gcctgtatgc cgtccagcgc gtgccggtcg ggccgatcga gcgcctcctg ctgctggccg
     1801 ccgaattcgg cgtcgacctg ccgcccgagc tcggcgcgcc gcctgacccg gccgccgtcc
     1861 ccgacggcga ccgcaacccg cggatcctcg acttctccgt caccctcgtc cgcgacggcg
     1921 agagcaccga tctcggcccc aagaaccgcg gcgatgtcgt ccaggcccgc ctcggcgacg
     1981 tcctgaagat cgccaccaac gccccgccga ccgacctcca ggagttcctc gtccccgtca
     2041 acggcggcgc cggcggcacc gagatccagg ccgagtccta tgacggatcg tggtaccgca
     2101 gctggggcac cctgcaggcc ggcggcagcg acgacccgct cagcgagaac gagtggcgca
     2161 tggagatggg cacccaggac gaggaccttt cgcctcccga cggccgcgcc accctgttct
     2221 acgtgctgcg agacggccgc gtcggcctcg actggtggtg gatctccgtc gaggtcgagc
     2281 cggcgtcgtg acttctggct cagctgtctc gacagtgccg tgaaaccccg cgattttgcc
     2341 ggcccaggcg cgttcggcga agcaccaccg ccgccacgcc ccgcgcagac caccgcttga
     2401 ccccggcagg cgaggacgct atcagcctgg ggcccctcgt gtcgctctcc ctcgccatgc
     2461 tgctcgccgg cctcacggtg agccagaacc ccacggtcaa cccggagacc gggctcgcgc
     2521 gggacgtgca gcagcgcatc gagccgcccg tcgtcacgcc ccggccctcg cccgacgtcc
     2581 agtcgccctc gagcaccgcg tcacccggca tgcggccgcc gtcgacgacc gacccgggca
     2641 tgggcacgag caccgcgccc ggtcgcacgc cgctgcgccg ccccggcacc gagccgatgc
     2701 cgagctcccc gccgacgacc ggcacgcctg ctcccgtccc cggccagcag accccgcgcc
     2761 cgaccagccc cggcgagctc tacccgctct ccttcgccac cgagtcgctc gagctgagcc
     2821 aggtgttgaa gacggcgctc gagaacaacc tcgacctgcg ctcgaccgcc ttcgacgtcg
     2881 cgatctccga gaagaacatc atggtggcgc tcggcgccta cgacgtgttc atgaccggct
     2941 cgctgtacgc cgccaaggcc gtcactccgc agcgtggttc gcagctggcc ttcaacctcg
     3001 gccagacgca ggtcggcggc agcgtcggct tcgagcgcaa gttagagacc ggcggcaccg
     3061 tcaacctgac ggtcggcgcc tcgcggaccc tgttcctcca gcccttgcag atcggcaacg
     3121 ccgcggccgg caccgtcaac ctcggcagct acatcgtcgc gccgacgctg accctcacgc
     3181 acccgctgct gcgcaacgcc ggcctgcggg tcaaccgcgc cgacatcgac cgcgcccgcc
     3241 tggcgaccac ccgcgccgag gccggcgaga tgctcagcgc gcagacgatc gtccgcgacc
     3301 tgatcctcgc ctactgggac ctgctgttcg ccagccgcga cctcgacaac aagcgccgca
     3361 gcgccagcac caccgccgag cagcaccgcc gcacccaggc ggaggtcgcc gccggtcgca
     3421 agtcgcagct cgacctcgac acgatctatc agtcgctggt cgcccgcgag aacgacgtcg
     3481 tgctcgccga gaacgtcctg ctcgaccgca gcctgaccgt gcgcacgctg atgggtcagg
     3541 acttcgtcga ccgcaaggtc ctcggcatca tcccgcagac cgacccccag gcgatcgcgc
     3601 tgacgcccgt cgacctcaac gccaaggtca agcaggccct caagggccac ccccagctgc
     3661 gccagctcga gatcagcctc gccagccagc gcatcgacga gctcgtcgcc gccaacaagc
     3721 gactgccgca gctcgacgcc cgcgtcgtgt tcgccccgca gggccgctcg atcgacaccc
     3781 gcgcccagcc ggccgccggc acccccgccc tgtccggcaa ctggggccag gccttccaga
     3841 acttcttcaa caccggcgac caggccagca ccaccagcgg cctgctcgcc gactactcgg
     3901 cccgcgccga gctgaccttt agctgggaca tccagaaccg ggcccccaag gccacgcacg
     3961 agcgcatcat cctcgagatg cgccgcgccg aggcccagct gcggcgcaac cggcagaaca
     4021 tcgccatgtc ggtcatccgt gccgtcaacg cccagcgcac cgcgggcgcc cgcatgacga
     4081 tcaccagcga gtcggtgcgc ctggcccagt cgaacctgcg cgccgaggag gcccgcaacc
     4141 gcgtcggtcg ctcgaccagc tatgacgtgc tgttccgcca ggacgagctg gcgatcgccg
     4201 agttcaacgc cctcaacgcg cagatcgact acctccgcgc gaccgtcgac ctccagatgt
     4261 tgaccggcga gctgctgccg tcctacgggc tcgaactcgc cagtcggtcg gcgcggatct
     4321 cgcaggatga tcaggccaac gccgaccccg gccgttacga gtgagcgccc gctggatctt
     4381 cagcgagcac ttgtcgggta aaccggacgc gtgatagctt cgttttgagc tcgcccccgg
     4441 gcagcaagag gaccagtcca tgtctgagat cgccgacaac aagtgggaag ccgaagccgc
     4501 caagccgccc gtcagcacgg tctcgggcat cgcccagatc ctgctcatcg cctgtttcgc
     4561 gggcgtgttc gtgtacatcc tccagggctc cgcccgcggc ctcttctccg agaagaagga
     4621 cggcctcgcc gagaactacg gcaagatgac caacgaagcc ggcaaggagt gatctcctgc
     4681 gtggctcgag gccgtgcgcg ctcgcgcgcc ggcctgtgat cgcgacagcc gaaccgagag
     4741 agctggtcca cccgggccgg ctcttttcat tttagtgccc cggctcagcc gagcccgata
     4801 aacgccagca tctgcccgat ccccgcgccg tcgcggcgat acgagaagta gcgcgttggg
     4861 tcgctgaaag tgcagccgcc gacccgggag atcgctcccg ccgggacacc cgccgcgatc
     4921 aactgcgcgc gcaccgtcgc cacgagatcg acgtgcggct tgcgggtccc ctcgcggcgc
     4981 acgtgctccg ccgggaattg caccgcgacc tcctcgccga cctcgaaggc ctcgacctcg
     5041 atgcatggcc cgatcgccgc cagcaaccgc gccggcgcga catgttcggc gagcacgccg
     5101 accgtcgccc cggcgatgtc ggcgaccgtg cccttccacc cgctgtgcac cgccgccgcg
     5161 accgtgccgt ccacgtccgt cagcaagatc ggcacgcagt cggccgtcag caccccggcg
     5221 acgagcggcc cgtcctcgcg gtggacccac aacgcgtcgg cctcgctcgc ggccgtcacg
     5281 tcgcgggcgc gcagcaccgt cttgccgtgg acctgccgga cctgccgcag atcctcgacg
     5341 cgaaagcccg cggccgcggc gacgcggcga tagttctcgg cgaccgccgc cggatcatca
     5401 ccccagcggc gcccgaggtt gaggctcgcg tgtcgtccct cgctgactcc accgaggcgg
     5461 tcggtgaagc cgtgcacgca gcccccgaac gccggcaccc gatcgatcac gcggcccgct
     5521 ccgggctcag ctgctcgacc agctgcacca gatccggcgg cagcggcgac gagaagtcga
     5581 gcgccgcgcc cgagatcggg tgcacaaagc cgagctccgc ggcatgtaag gcgtggcgcc
     5641 cgagcgccgc ggagatcgct cgcctcgcgg gcggcccccc gttcccgccg tacatcgcgt
     5701 cggcgacgat cgggtgaccg agcaggcggc agtgcatgcg gatctggtgc gtgcgacccg
     5761 tctcgaggcg aaacgccagc aacgccgcgt cacgcagcgg acgcagcact tccaggtgtg
     5821 tgatcgcctc gcgaccctcg tcgatgtgcg gcgagaagcg acgccggtcg ccggggtcgc
     5881 gaccgtgtcg cgtccgcagg ctcttcttca cgaagcccgg cacgccccag acgatcccga
     5941 ggtagcggcg gcgcaggtcg tgcacccgga actgcgccgc gagcccctcc tgcgcgcgct
     6001 cgttgcgagc gacgacgatc agcccgctgg tgtcgcggtc gaggcgatgg acgatgcccg
     6061 ggcgggtctc gtcgttgccc ggcagcaccg gcaggtcctt ggcggagatc ccgaggtgat
     6121 gcagcaccgc gttgaccagc gtgccgcgat cgtgtcctgg accggcgtgc acgaccatcc
     6181 ccgccggctt gtcgatcacc agcaagtgct cgtcctcgtg gacgatcgtc agcggcaggt
     6241 cctcggcgac cgcctgcacc ggtttgataa tttccagctc gagctcgacg gcgaccgcgg
     6301 cctcgacgac gcgcgagggc ttgcccgcga cggcgccggc gaccttcacc ccgccggccg
     6361 cgaaggcccg cgcgagctgg gagcgcgagc agttgtggcc tgctcgctgg agggccgcga
     6421 cgagcgcggc gtcgaggcgc tggccgagcg cctcgggtcc gagcgacaca cgaaggcggc
     6481 tcaccagatc ggggtcttga ggctggccat cggcttcacg acgcggtcga cgatcgcccg
     6541 gtcgtagtag ttgcgacgga acagctcggg ggtcacgcgg ccgcggaaca ggtcgcgacc
     6601 ttcggtgatc tcctcggcga gcgcctcgaa gaagttgtcg ttgatcagcg cctcctcgat
     6661 cttctgcttg ttgtagagct ggatatccga ggcgatcgcg cgggcgagct gctgcgcgcg
     6721 ggcgtcgttg tcgatgggcg tggcggccat ggtcggcagc atgccacgtc gcctccggcg
     6781 ccgccagacc tcgacaaacc cggcaaaccc tccccagcgg ccccgccccc tcctggaccg
     6841 gcgcgccgag cgagatctcc cgcgccccgg ccatatgggg ctaccatggc cgggcgtgtc
     6901 cggctcgaac ctctacgcca gcctgagctc gctgctggcc gccgcggtgg cgatcgccat
     6961 cggcatcagt gttttcctgc gtgaccgctc gcgcgaccag tttcgcctgt tcgccgcctg
     7021 ctgcttgaac ctcggcctgt ttcacctcgc caggttcttc gccggcttcc ccggcttgcc
     7081 gctgtcgatc tgggtcgccc agaccgtctc gctgttcctg ccctacaccg ccgaccgctg
     7141 cttctccggc ttcatcccgg cctccggcgc cccgccgagg aacctcgttc aggcgccgat
     7201 cctcgctctt ctcctgatcg cccaggcgct ctcgctgctg ttccccggcc tctccgacga
     7261 ccccgcgtgg gggatcgtcc cgttcctcgt ccaggcctac gtgctcggcg ggctgctcta
     7321 cgccgcgatg cggatgtggc gggcagcccg ggccgccgag ggcaccgcag ccgccccgcg
     7381 cctgcgctac ctgttctacg ccagcgtgct cgcgctggcg ctcggtcagc ccggcatccc
     7441 ggtcgtcggt gccaccgtca ccgccgtcta cctctacttc atggcgcaga ccctggtgcg
     7501 cgagcgactg ctcgacct
//
LOCUS       DASWNJ010001630         1871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_624954, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001630 DASWNJ010000000
VERSION     DASWNJ010001630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624954"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..264)
                     /locus_tag="VGB85_27100"
     CDS             complement(94..264)
                     /locus_tag="VGB85_27100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137794.1"
                     /translation="MSSRPFVVNVDTAYCIADNPEDCGNDAFDPEVRTQLIRENGGGS
                     RYWFSLIVLPAG"
     gene            complement(261..482)
                     /locus_tag="VGB85_27105"
     CDS             complement(261..482)
                     /locus_tag="VGB85_27105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137795.1"
                     /translation="MWSDNLGATTKATTDSAGVNSDATGTSGETDPTDATTVPTGGDE
                     TATPPTGGELPTVTCAPLPAGVVGVECEL"
     gene            complement(566..>1871)
                     /locus_tag="VGB85_27110"
     CDS             complement(566..>1871)
                     /locus_tag="VGB85_27110"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0137796.1"
                     /translation="GGAWSRADPAHRGRVLWAFAQKIREHAEALAWLETRDSGKPIAE
                     SRADITGTAEILEYYAGLASQIFGQTTRVPGNNLAMVLREPVGVVGQITPWNFPLYVG
                     AWKFAPALCCGCSVVLKPSELTSLTMLAAAELALAAGVPPGVFNVVCGTGPVAGAALA
                     SHPDVDVLAFTGGVITGRKVLHARADLLRPTQLELGGKSPNIVFDDADLERATAGAAF
                     GIFYNQGENCNAGSRLLVQARIYDEFVAGLVERAKRIRVLPPLDERSQMGALISAEHA
                     RKVHGHVEAALAEGAQLLCGGSPPGDSHFARGHWYLPTVLAQVQPGHRVFRDEVFGPV
                     VTVTRFETDDEAIALANGTEFGLAAGAWTGSVQRALRCTQELQSGYVWINNYNPTPVE
                     VPFGGVKSSGFGRDCGPQAVDTYTTWKTVLWSLQPFEDWYRS"
BASE COUNT          275 a          659 c          658 g          279 t
ORIGIN      
        1 ttccagatgt ttttatcgaa gccgatccac ccggaggagc tggtcaaggt cgtcgccgag
       61 ctgctgagga ccgaccgttg accggcgcgg cgctcacccg gcgggcagca cgatcaggct
      121 aaaccagtaa cggctgccgc ccccgttctc gcggatcagc tgcgtgcgca cctcgggatc
      181 gaatgcatcg ttgccgcaat cttcggggtt gtcggcgatg caatatgcgg tatcgacgtt
      241 gacgacgaag gggcgagagc tcacagctcg cattcgacgc cgacgacacc ggcgggtagc
      301 ggcgcgcatg tgaccgtcgg cagctcgccg ccggtcggcg gcgtggcggt ctcgtcgcct
      361 ccagtcggca cggtcgtcgc gtcggtcgga tcggtctcgc cggacgtgcc cgtcgcgtcg
      421 gagtttactc cggcgctatc ggtggtcgcc ttcgtggtgg ccccgaggtt gtcggaccac
      481 aacccacagc gcgcacgtca gcgcgaatcc ccatcgccga ttcatcatgc ccgccacggt
      541 acacgaccgc gtgggatcgc gaccgtcagc tccggtacca gtcctcgaac ggctgcagcg
      601 accacaacac cgtcttccag gtcgtgtagg tgtcgacggc ctggggcccg cagtcgcggc
      661 cgaagccgga cgacttgacg ccgccgaacg gcacctcgac gggcgtcggg ttgtagttgt
      721 tgatccacac gtagccggat tgcagctcct gcgtgcaacg cagggcccgc tgcacgctgc
      781 cggtccacgc accggcggcg aggccgaact cggtcccgtt ggcgagcgcg atcgcctcgt
      841 cgtcggtctc gaagcgcgtc accgtcacca caggcccgaa cacctcgtcg cggaacacgc
      901 ggtgcccggg ctgcacctgc gcgagcacgg tcggaagata ccagtgcccg cgggcgaagt
      961 gcgagtcgcc cggtggggac ccgccacaca gcagctgcgc gccctcggcg agggcggcct
     1021 cgacgtggcc atgcaccttg cgcgcgtgct cggccgagat cagcgcgccc atttgactgc
     1081 gctcgtcgag cggcggcagc acgcggatcc gcttcgcccg ctcgacgagg ccggcgacga
     1141 actcgtcgta gatcctcgcc tgtacgagca ggcggctgcc cgcgttgcag ttctcgccct
     1201 gattataaaa gatgccgaag gccgcgccgg cggtcgcacg ctcgaggtcg gcgtcgtcga
     1261 acacgatgtt gggcgacttg ccgccgagct cgagctgggt cgggcgcagc aggtcggcgc
     1321 gggcgtgcag caccttgcgg ccggtgatca cgccgccggt gaacgcgagc acgtcgacgt
     1381 ccgggtggct cgccagcgcc gcgccggcga ccgggccggt gccgcacacg acgttgaaca
     1441 cgcccggcgg gaccccggcc gcgagcgcga gctcggcggc ggcgagcatc gtcagtgagg
     1501 tcagctcgct cggcttgagc accacgctgc agccgcagca cagcgccggc gcgaacttcc
     1561 aggcgccgac gtacagcggg aagttccacg gcgtgatctg cccgaccacg ccgaccggct
     1621 cgcgcaggac catggcgagg ttgttgccgg gcacgcgggt ggtctggccg aagatctgcg
     1681 aggcgaggcc cgcgtagtac tcgaggatct ccgcggtgcc ggtgatgtcc gcgcgcgact
     1741 cggcgatcgg cttgccgctg tcgcgggtct cgagccacgc gagcgcctcg gcgtgctcgc
     1801 ggatcttctg cgcgaaggcc cacagcaccc gcccgcggtg cgcagggtcg gcccgcgacc
     1861 aggccccgcc g
//
LOCUS       DASWNJ010001631         2403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_665321, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001631 DASWNJ010000000
VERSION     DASWNJ010001631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665321"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1510)
                     /locus_tag="VGB85_27115"
     CDS             complement(<1..1510)
                     /locus_tag="VGB85_27115"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY0137797.1"
                     /translation="MAPTSATGPVPLLPLRGVSILPGLTAPVDLSRQSPAMQAVRRAG
                     ERRDPRNPRPENEPRGRIVVGILRENTATPDLDQLYPVGVACEVVQVLPGTPGRMSVV
                     LRGIGRVRLLDLYSERDHLVVRVETAVDTMRTSTLAYALSGALQDLVKQHDTLLPTAA
                     KTKQRAQGLAAILAERSPGMVADLCAAHLDLSDEDKLSVLLETEVSDRLRKVIELASH
                     RTNVLQVKRDLDRHVREHLSKHEHDALLRHKLRAIQSELGESEGEADERGLRELEQRL
                     TALELPEEGRAAANRELNRLKRMHPQSGEAAIARTYLEWIADLPWGAQAATPDKLDLT
                     RARALLELHHHGLEKVKKRVLEYLSVRKLAPDKRGPILCLVGPPGVGKTSLGRSIADA
                     LGRKYVRISLGGVRDDAEIRGHRRTYIGALPGRFIQAMKRAGTSNPVIVLDEVDKLVG
                     ASLRGDPAAALLEALDPEQNTAFRDHYLDIDYDLSKVIFLCTANELSGIPDVL"
     gene            1554..>2403
                     /locus_tag="VGB85_27120"
     CDS             1554..>2403
                     /locus_tag="VGB85_27120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137798.1"
                     /translation="MEAPPRGPLALSAPAALAGGVGLVLLAGPGLPLAWGLLPAALAL
                     ALAVVVGRWRTRDRAPEDLEAAPGSSPAPWLLAPLLVLPAIVLVAVAARVIAAMCVAS
                     PPAIAALVVLCVLAPAIGGARRGLGIGLALAIAALAVAAAIGAARFEAAGPGARGFAH
                     SGPILGIHPFQSTAIVIDGFGPFDLPINDYVEPDGSRGYKPAELAEALQRDLAAIAEQ
                     QFADGPARAYQAFAGARVEAVLLPAIQERLDRPVEAGTSEPRLLVWSGTTGARSRVEF
                     LCPGTRN"
BASE COUNT          268 a          813 c          958 g          364 t
ORIGIN      
        1 gcaggacgtc cgggatcccc gacagctcgt tggcggtgca caggaagatc accttcgaga
       61 ggtcgtagtc gatgtcgagg tagtggtcgc gaaacgcggt gttctgctcg gggtcgaggg
      121 cctcgagcag cgccgcggcc gggtcaccgc ggaggctggc gccgacgagc ttgtcgacct
      181 cgtcgagcac gatgacgggg ttcgaggtcc cggcgcgctt catcgcctgg atgaagcggc
      241 ccggcagcgc gccgatgtac gtgcgccggt ggccgcggat ctcggcgtcg tcgcgcacgc
      301 cgccgagcga gatccggacg tacttgcggc cgagcgcgtc ggcgatgctg cggccgagcg
      361 aggtcttgcc gacgccgggc gggccgacga ggcacaggat cgggccgcgc ttgtcgggcg
      421 ccagcttgcg cacgctgagg tactcgagca cgcgcttttt gaccttctcc aggccgtggt
      481 ggtgcagctc gagcagcgcg cgggcgcggg tgaggtcgag cttgtcgggg gtcgccgcct
      541 gggcgcccca cgggaggtcg gcgatccact cgaggtaggt gcgggcgatc gccgcctcgc
      601 cgctctgcgg gtgcatgcgc ttgaggcggt tgagctcgcg gttggccgcg gcgcgcccct
      661 cctcgggcag ctcgagggcg gtcaggcgct gctcgagctc tcgcaggccg cgctcgtcgg
      721 cctcgccctc gctctcgccg agctcggact ggatcgcgcg cagcttgtgc cgcagcaggg
      781 cgtcgtgctc gtgcttcgac aggtgctcgc ggacgtggcg gtcgaggtca cgcttgacct
      841 gcagcacgtt ggtgcggtgc gacgcgagct cgatgacctt gcgcaggcga tcgctgacct
      901 cggtctcgag cagcaccgag agcttgtcct cgtcggagag gtcgaggtgc gcggcgcaca
      961 ggtcggcgac catgccgggc gaccgctcgg ccaggatcgc agcgaggccc tgggcccgct
     1021 gcttggtctt ggcggcggtc ggcagcaagg tgtcgtgctg cttgacgagg tcctgcagcg
     1081 cgcccgagag cgcgtaggcg agggtcgagg tgcgcatcgt gtcgaccgcg gtctcgacgc
     1141 ggacgacgag gtgatcgcgc tcgctgtaga ggtcgagcag gcgcacgcgg ccgatgcccc
     1201 gcaggaccac ggacatgcgg cccggggtgc ccggcaggac ctggacgacc tcgcaggcga
     1261 cgccgaccgg gtacagctga tcgaggtccg gggtggccgt gttctcgcgc aggatcccga
     1321 cgacgatgcg gccgcgcggc tcgttctccg ggcgcgggtt acgagggtcc ctgcgctcgc
     1381 ccgcgcggcg gaccgcctgc atcgccggag attgccggct caggtcgacc ggcgccgtca
     1441 ggcccggcaa gatggatacg ccgcggagcg gcagcagcgg cactgggccc gtcgcgctgg
     1501 tcggtgccat gcgggcgaaa gactaaccga ttttcctctg atacaaggag cgcgtggaag
     1561 cgccgccccg tggtccgctc gccctgtccg cccccgcggc gctggcgggt ggcgtgggcc
     1621 tggtgctgct ggcgggtccc gggctgccgc tcgcgtgggg cctgctgcct gcggcgctgg
     1681 cgctggcgct ggcggtcgtg gtcggccgct ggcgcacgcg ggatcgggcc ccggaagatc
     1741 tcgaggcggc gcccggatcg agcccggcgc catggttgct ggcgccgctg ctggtcctgc
     1801 cggcgatcgt gctcgtcgcc gtcgcggcgc gggtgatcgc ggcgatgtgc gtcgcgtcgc
     1861 cgccggcgat cgcggcgctg gtggtcctct gcgtgctcgc gccggcgatc ggcggggcgc
     1921 ggcgcgggct ggggatcggg ctcgcgctcg caatcgcggc gctcgccgtg gcggcggcga
     1981 tcggcgcggc ccgcttcgag gcggccgggc cgggcgcccg cggcttcgcc cactccgggc
     2041 cgatcctcgg catccacccg ttccagagca cggcgatcgt gatcgacggc tttgggccct
     2101 tcgatctgcc gatcaatgac tacgtcgagc ccgacggctc gcgcgggtac aagccggccg
     2161 agctggcgga ggcgctacag cgcgatctcg ccgcgatcgc cgagcagcag ttcgcggacg
     2221 ggccggcccg ggcctaccag gcgttcgccg gggcgcgcgt cgaggcggtc ctgctcccgg
     2281 cgatccagga gcggctcgat cgcccggtcg aggctggcac gagcgagccg cggctgctgg
     2341 tgtggtcggg gaccacgggg gcgcgctcgc gggtcgagtt cctgtgtccc ggcacgcgca
     2401 acc
//
LOCUS       DASWNJ010001632         3168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_594737, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001632 DASWNJ010000000
VERSION     DASWNJ010001632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594737"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VGB85_27125"
     CDS             <1..645
                     /locus_tag="VGB85_27125"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0137799.1"
                     /translation="VFGDPGPGDQLAVCLGGLDIATQARARLASGAVFRADVRVDTPT
                     GEHWFDTVVRRSLDPVTGQPAVLVTQRDVTERRAELRELEHSRELLAGQAQALRVLAA
                     PVIEIGPGVLAMPLIGRLDHARVEVALAALVGAAGERASHVVLDLTGADAADLAGTGG
                     LVRVVRVLRLRGIRVALSGIRPALARALVDADLDLTGLACFATVADALASVDRR"
     gene            complement(935..>3168)
                     /gene="gcvP"
                     /locus_tag="VGB85_27130"
     CDS             complement(935..>3168)
                     /gene="gcvP"
                     /locus_tag="VGB85_27130"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEY0137800.1"
                     /translation="LDESGVIAAARDAGALVVVATDLLALTVLRAPGEFGADIAVGSA
                     QRFGVPLGYGGPHAAFLATREAYKRALPGRVIGVSRDATGKRAYRMAMQTREQHIRRE
                     RATSNICTAQVLLAIMAGMYAVYHGPQGLRTIGRRVHGLTAVLAEGLRRLGHSVGEAA
                     FFDTLRVELVHRPAAAVLAAARERGFNLRPIDATTVGVSLDERSTIAEVDALLATFSD
                     NAAVAAPGSEIRQAAEIAEGLDLAVPQAFARTSEFLTHPVFNGYHSEHDLLRYIHRLQ
                     GRDLSLTSAMIPLGSCTMKLNATSEMQPISWPALADIHPFAPRDQAQGYAAMFASLEA
                     MLCEITGFAAVSLQPNAGSQGEYAGLLVIRAHHHANGQQHRDICLIPTSAHGTNPASA
                     VMAGFQVVAVACDVNGNVDLADLEAKAAKHADKLAALMITYPSTHGVFEVQIREICET
                     VHRHGGQVYLDGANMNAQVGLCRPGDFGADVCHLNLHKTFCIPHGGGGPGMGPIGVAA
                     HLAPYLPGHPVITCGGVTPIGPVAAAPWGSASILTISYVYILMMGAAGLTQATRMAIL
                     NANYMAKRLEKHYPVLYRGKNGTCAHEFILDLRPLKATAGVEGEDVAKRLMDYGFHAP
                     TMSFPVAGTLMIEPTESEPLAELDRFCDAMIAIREEIRKIEDGTWPRDNNPLKHAPHT
                     AEAVTASAWDRPYPREVAAFPAKWVREHKFWPFVGRIDNAWGDRNLVCSCPPMEDYAE
                     AAE"
BASE COUNT          412 a         1181 c         1116 g          459 t
ORIGIN      
        1 gtgttcggcg accccggccc cggcgaccag ctcgccgtgt gcctcggcgg cctcgacatc
       61 gccacgcagg cgcgcgcccg tctcgccagc ggcgcggtgt ttcgcgccga tgtccgcgtc
      121 gacacgccga ccggcgagca ctggttcgac accgtcgtgc gccgcagcct cgaccccgtc
      181 accgggcagc cggcggtcct cgtcacccag cgcgacgtca ccgagcgccg cgccgagctg
      241 cgcgagctcg agcacagccg cgagctgctc gccggccagg cccaggcgct gcgcgtgctc
      301 gcggccccgg tgatcgagat cgggccgggc gtgctcgcca tgccgttgat cggccgcctc
      361 gaccacgcgc gcgtcgaggt cgccctcgcg gccctggtcg gcgccgccgg cgagcgagcg
      421 agtcacgtcg tcctcgacct caccggcgcc gacgcggccg atctggccgg caccggcggg
      481 ctggtccgcg tcgtccgggt cctgcgcctg cgcggcattc gggtcgcgct cagcgggatc
      541 cgcccggccc tcgcgcgggc gctcgtcgac gccgacctcg atctcaccgg gctggcctgc
      601 ttcgccacgg tcgccgacgc gctcgcctcc gtcgaccgcc gctgacgccc cgcacacgcg
      661 cgtcacatca cggtgtcatc gaaccgtccc cgcaatgctg cattggcagc cacgcggcgc
      721 acgcaagccg acgcgcggcc cgctcgccgc gcgtgccggc cggccttgag gtatgtcgcc
      781 gttatgtcag ctgtccgcga ggacagttgc gaggcgtcgc cgctatggcc gattcaccgc
      841 ggcggctatc atgacacttc gcaaccgccg gccgtgcgcc cgaccacagg ggtgggcgac
      901 gcaccgacgt tgtccgtcgc ggtgcctcgg ggccctactc ggccgcctcg gcgtagtctt
      961 ccatcggcgg gcagctgcac accaggttgc ggtcgcccca ggcgttgtcg atgcgcccga
     1021 cgaacggcca gaacttgtgc tcgcggaccc acttcgccgg gaacgcggcg acctcgcgcg
     1081 gatagggccg gtcccacgcg ctcgcggtga cggcctccgc ggtgtgcggc gcgtgcttga
     1141 gcgggttgtt gtcgcggggc caggtgccgt cctcgatctt ccggatctcc tcgcggatcg
     1201 cgatcatcgc gtcgcagaag cggtcgagct cggcgagcgg ctcgctctcg gtcggctcga
     1261 tcatcagcgt gccggcgacc gggaacgaca tcgtcggcgc gtggaagccg tagtccatca
     1321 ggcgcttggc gacatcctcg ccctcgaccc cggcggtcgc cttgagcggc cgcaggtcga
     1381 ggatgaactc gtgcgcgcag gtgccgtttt tgccgcgata cagcaccggg tagtgcttct
     1441 cgagccgctt cgccatgtag ttggcgttga ggatcgccat gcgcgtcgcc tgggtcagcc
     1501 ccgccgcgcc catcatcagg atgtacacgt aggagatcgt caggatgctc gcgctgcccc
     1561 acggcgccgc cgcgaccggg ccgatcggcg tcacgccccc gcaggtgatc accgggtgac
     1621 ccggcaggta cggcgcgagg tgagccgcca cgccgatcgg gcccataccc gggccgccgc
     1681 cgccgtgcgg gatgcaaaac gtcttgtgca ggttgaggtg gcagacatcc gccccgaagt
     1741 cccccggccg gcacagcccg acctgcgcgt tcatattggc gccgtcgagg tagacctgcc
     1801 cgccgtgccg atgcacggtc tcgcagatct cgcggatctg gacctcgaac accccgtgcg
     1861 tcgacgggta ggtgatcatc agcgcggcga gcttgtccgc gtgcttggcc gccttggcct
     1921 cgaggtcggc gaggtcgacg ttgccgttga cgtcgcacgc gaccgcgacc acctggaagc
     1981 ccgccatcac cgcggacgcc gggttggtcc cgtgcgccga ggtcgggatc aggcagatgt
     2041 cacgatgctg ctgcccgttg gcgtggtggt gcgcgcggat gacgagcagc cccgcgtact
     2101 cgccctgcga gccggcgttg ggctgcaacg acactgccgc gaagccggtg atctcgcaca
     2161 gcatcgcctc gagcgaggcg aacatcgccg cgtagccctg ggcctggtcg cgcggcgcga
     2221 acgggtggat gtcggccagc gccggccacg agatcggctg catctcggag gtcgcgttga
     2281 gcttcatcgt gcacgagccg agcgggatca tcgcgctcgt cagcgacagg tcgcggccct
     2341 gcaggcgatg gatgtaacgc agcaggtcgt gctcgctgtg gtacccgttg aacaccgggt
     2401 gcgtcaggaa ctcgctggtg cgcgcgaagg cctgcggcac cgcgaggtcg aggccctcgg
     2461 cgatctccgc cgcctggcgg atctcgctgc ccggagccgc gaccgcggcg ttgtcggaga
     2521 aggtcgccag caaggcgtcg acctcggcga tcgtcgagcg ctcgtcgagc gacacgccga
     2581 ccgtcgtcgc gtcgatcggc cgcaggttga agccgcgctc gcgcgccgcc gccagcaccg
     2641 ccgccgccgg acgatgcacg agctcgaccc gcagcgtgtc gaagaacgcc gcctcgccga
     2701 ccgagtgccc gaggcgccgc aggccctcgg cgagcaccgc cgtcagcccg tgcacgcgcc
     2761 ggccgatcgt gcgcagcccc tgcgggccgt gatagacggc gtacatgccg gccatgatcg
     2821 ccagcagcac ctgcgcggtg cagatgttgc tggtcgcccg ctcgcggcgg atgtgctgct
     2881 cgcgggtctg catcgccatg cgataggccc gcttgccggt cgcgtcgcgc gacacgccga
     2941 tcacgcgacc cggcagcgcg cgcttgtacg cctcacgcgt cgccaggaac gccgcgtgcg
     3001 gaccgccgta gccgagcggg acgccgaagc gctgcgccga gccgaccgcg atgtcggccc
     3061 cgaattcacc gggcgcgcgc aggaccgtca gcgccagcag gtcagtggcg acgaccacca
     3121 gcgcgccggc gtcgcgcgcc gccgcgatca cgccggactc gtcgagca
//
LOCUS       DASWNJ010001633         1554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_99818, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001633 DASWNJ010000000
VERSION     DASWNJ010001633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99818"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1457
                     /locus_tag="VGB85_27135"
     CDS             195..1457
                     /locus_tag="VGB85_27135"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEY0137801.1"
                     /translation="MTVEVTVNNPLHDARTQLSRAVEVLGYSTGIFDMLATPRRELTV
                     SVPLRRDDGSVELLVGHRVQHNLSRGPSKGGLRYSPFVTLDEVRALAMWMTWKCALVD
                     VPYGGAKGGVRFDPKQYSSSEVQRVTRRYTSEIMPMIGPAQDIPAPDIGTDEQVMAWM
                     MDTYSVSKGHTVLGVVTGKPVALGGSLGRASATSRGVVYVALAAMRHRGLSPEGSAAA
                     VQGFGKVGRGAARFLREAGVTVVSVSDQYGAVYAAEGLDIPALESHVDATGSVLGFAS
                     STPLDHPDDLLYLPVDLLVPAAVEGVITESNAHRVTAAVVVEGANGPTSPAADDVLTA
                     NGQLVVPDILANAGGVIVSYFEWVQANQAYWWSEAEVEQRLEERMVRAWERVRDHAAR
                     HDIPMRLAATSMAVETVTTAHLARGLYP"
BASE COUNT          235 a          519 c          566 g          234 t
ORIGIN      
        1 cacgccgagc acggatcatc tgattgggtc aggtgatcag catggggcga ccccgcccgc
       61 cccgtcaagg ggttcggggc gatcgcatgc aagagggcgg gacggcacgc ctggcgatgt
      121 gagtcgttcg tacgactaag atgcggtgag gtcaccatcc atgaggtgac gccgcaggtc
      181 gacaggggac cgtcatgacc gtcgaagtca ccgtgaacaa cccgctgcac gatgcccgga
      241 cacagctgtc ccgtgccgtc gaggtcctgg ggtactcgac gggcatcttc gacatgctcg
      301 ccacgccgcg tcgtgagctg acggtcagcg tcccgctccg gcgcgacgac gggtcggtcg
      361 agctcctggt cgggcaccgg gtgcagcaca acctctctcg cggcccgtcg aagggcgggt
      421 tgcgatacag ccccttcgtc acgctggacg aggtccgcgc gctcgcgatg tggatgacgt
      481 ggaagtgcgc cctggtcgac gtcccctatg ggggcgccaa gggtggggtg aggttcgacc
      541 cgaagcagta ctcgagcagc gaagtgcagc gggtgacccg ccggtacacc tccgagatca
      601 tgccgatgat cggcccggcc caggacatcc cggcgcccga catcggtacc gatgagcagg
      661 tcatggcctg gatgatggac acctactcgg tgagcaaggg ccacacggtg ctgggagtcg
      721 tgacgggcaa gccggtcgcc ctcggcgggt ccctcggccg ggcgagcgcg acctctcgtg
      781 gcgtcgtcta cgtcgccctg gccgcgatgc gacatcgcgg cctctcgccg gagggttcgg
      841 cggcggccgt ccagggcttc gggaaggtcg gtcgaggggc cgcacggttc ttgagggagg
      901 ccggcgtgac ggtcgtgtcg gtgagcgatc agtacggcgc ggtgtacgcg gccgaaggcc
      961 tggacatccc ggcgctggag tcccatgtcg acgcgacagg ctccgtgctg ggatttgcgt
     1021 cgtccacgcc gttggaccac cccgacgacc tgctctacct acccgtggac ctgctcgtcc
     1081 cggcggccgt cgaaggggtg atcaccgagt cgaacgccca tcgagtgacc gccgccgtcg
     1141 tcgtcgaggg tgcgaacggg cccacctcgc cggcggcgga cgacgtcctg accgcgaacg
     1201 gccagctcgt cgtgccggac atcctggcga acgccggtgg cgtgatcgtg tcctacttcg
     1261 agtgggtcca ggcgaatcag gcctactggt ggagcgaggc cgaggtcgag cagcggctcg
     1321 aggaacggat ggtcagggcg tgggagcgcg tccgtgacca cgcggctcgc cacgacatcc
     1381 ccatgcggct ggcggcgacc tccatggccg tcgagacggt cacgaccgct cacctggctc
     1441 gtggcctcta cccgtagccg cgagccggcg ccggcctggg cccgctgacc gcaccgccct
     1501 cggggtacgg cgcgtcgacg gggaacgccc agccgactcc ggcggaccgc cgcg
//
LOCUS       DASWNJ010001634         5130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_411701, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001634 DASWNJ010000000
VERSION     DASWNJ010001634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411701"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VGB85_27140"
     CDS             <1..366
                     /locus_tag="VGB85_27140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137802.1"
                     /translation="IVKKANVAVTELIQNVLENIVDPTSELVLDMRVDGDALDIRVRN
                     RATEVQYEAVRARVDALAQAADPKRLLGETLRARRADQLKGGLGLLRLVSENRFQLEA
                     SYEQEHLTVHAAFPLRGKT"
     gene            363..770
                     /locus_tag="VGB85_27145"
     CDS             363..770
                     /locus_tag="VGB85_27145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137803.1"
                     /translation="MNILTYGEIMDGGASVFHCVVREDVSSLFVTFSGVVRVDNPARH
                     LGDLLTDLRHILPGRTVEATTLDFTELEFCNSNGFYIIMDIIEIVYGATTGPVLVRRL
                     EEDDWQQETLPILLNLDEPAVAARTSFENVSEV"
     gene            785..1822
                     /locus_tag="VGB85_27150"
     CDS             785..1822
                     /locus_tag="VGB85_27150"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY0137804.1"
                     /translation="MPSLLDLLTPVLLRVDAAGGLEGADEPSRAWLAGREDRSLAKLC
                     GVDEPAQLVEAQGPWRALALPEGAYLVSGSAWQSSEAIAGRLAKTSLQDKQVLLNVMP
                     SATAEELAARTLQPKAYRESTILFIDAVQFSRLAARVDPVSCLKQLDFYFSLFDQVTA
                     AFGVEKVKTIGDAYMGVAGVPHRRAAHAVDATLAALRVARAIAARPGPVVDDWEWSFR
                     LGLHSGPCITGVLGARRQVFDLWGDTVSVAAQVQRVGRPDTVSVSGPTVELIGGFFDI
                     GFDGTTVIHHSGEIAVHVVRGIRPELLADAEEGIVNAEFCKRYEALFGEACPEAAWRP
                     QAASAALLGAL"
     gene            1982..3511
                     /locus_tag="VGB85_27155"
     CDS             1982..3511
                     /locus_tag="VGB85_27155"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0137805.1"
                     /translation="MKPGAFDESSTAHPVAISEDLLASPPAVFPGQLLGDRYHVGERI
                     GGGGMADVFRARDPYLYRDVAIKVIKPGMASGEMCGRMLQEGRATAAIDHPHLLRVLD
                     FGRVGATVYLVTDLLRGCSLAEHLRSLADARLGWREAIELLLPALDALAQIHDRGFVH
                     RDLKPDNLFVHHRDGREALIVLDLGIAKVSPAFRGGGALNTTESGRVLGTPAYMSPEQ
                     AGGKPLDHRTDVYSIGVTLYRMLAGRLPFEARAGEGPIVLMAHHLYDPPPRLDEVVPG
                     LPAPLVEVVMRSLAKAPAERPQTMRALAQALREALPAEPAGDAAAPTVARVKRTRSVG
                     WLSGGVVLLAALILLRGVQEVAATPAVAGSPLADDDGGADEAEEMEMVRPSSPSFTLA
                     LLPDRPPVARAPSVALPERRGSARRKSPHAGVARVLAGVADGVGDCIERRGGLDQETL
                     QVTWDVRPDGRVIASRLPVGTRTTLAGCVQALVSGLQFPPGTGLHVEHTFKRAPEARR
                     P"
     gene            3508..4377
                     /locus_tag="VGB85_27160"
     CDS             3508..4377
                     /locus_tag="VGB85_27160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137806.1"
                     /translation="MSAAALLVWLTAWGPAAPEVPAEARAHDDRGLAYIEGGDAVAGV
                     AELERAYALMPDPLRYRVGRSEVLGSLRGALTDLYEATGDAGHLRRLHVHLLRHLEAL
                     IVALGDGVTADDVAGTLVAVRKVEQQLARVPQVIAAPPRPVPRPRSVVPRPAPAMPAA
                     GEGPTRRQLYIAGGVSVGIGAVLLGVMAYGIAQQQGHGAAGWALKDRIDGRSITPAER
                     AELVGHLEGAHANRRLALATGLSSAGVSVLGVALLAHGRRRPDARERKTGVSLAPWWV
                     QAGVGVTLLLPLR"
     gene            4449..>5130
                     /locus_tag="VGB85_27165"
     CDS             4449..>5130
                     /locus_tag="VGB85_27165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137807.1"
                     /translation="MLLWTSCSASPVVDAPATDGTTGHEPTSADPPGMTPTSSAQPTT
                     AASSSTSSGSTAADTTTTTTEPQADTTGTTGAPAGCGDGEVGPGEACDDGLMNSDEGA
                     CTLHCQENTCGDGLVHVGVEACDHGVDNSDFVYNGCSTQCARTMFCGDGQVNGPEECD
                     RGEKNGTGDKDADAVPCSVSCNFESLLVFLSSRTYTVAELGGSAAAADERCEELAAAA
                     MLPNHMKFR"
BASE COUNT          749 a         1718 c         1919 g          744 t
ORIGIN      
        1 atcgtcaaga aggccaacgt cgcggtgacg gagctgatcc agaacgtgct cgagaacatc
       61 gtcgacccga cgagcgagct cgtgctcgac atgcgggtcg acggcgacgc gctcgacatc
      121 cgcgtgcgca accgggcgac cgaggtccag tacgaggcgg tgcgcgcccg cgtcgacgcg
      181 ctcgcgcagg cggccgaccc gaagcgcctg ctcggtgaga ccctccgggc ccgccgggcc
      241 gaccagctca agggcggcct cggcctgctc cggctggtct cggagaaccg cttccagctg
      301 gaggcgagct acgagcagga gcacctgacg gtgcatgcag cgttcccgct gcgaggtaaa
      361 acatgaacat cctcacctat ggcgagatca tggacggcgg cgcgagcgtg ttccactgcg
      421 tggtgcgcga ggacgtcagc tcgctgttcg tgacatttag cggagtggtc cgcgtcgaca
      481 acccggcgcg ccaccttggc gacctgctga ccgacctgcg ccacatcctc cccggccgca
      541 ccgtcgaggc gacgacgctc gacttcaccg agctcgagtt ctgcaactcg aacggcttct
      601 acatcatcat ggacatcatc gagatcgtct acggggcgac gaccggaccg gtgctggtcc
      661 ggcggctcga ggaggacgac tggcagcagg agacgctgcc gatcctgctc aacctcgacg
      721 agccggcggt cgccgcccgt acttcgttcg agaacgtgtc cgaggtgtag tccaggagaa
      781 gaccatgccg agcttgcttg atctgctgac cccggtgctc ctgcgtgtcg acgccgcggg
      841 cggtctcgag ggggccgacg agccctcgcg ggcctggctc gccgggcgag aggacaggtc
      901 gctggcgaag ctgtgtgggg tcgacgagcc ggcgcagttg gtggaggcgc aggggccgtg
      961 gcgcgcgctc gcgctgcccg agggtgcgta cctggtcagc ggcagcgcgt ggcagtcgag
     1021 cgaggcgatc gccgggcggc tggcgaagac gagcctgcag gacaagcagg tgctgctcaa
     1081 cgtgatgccg agtgcgacgg cggaggagct cgcggcgcgg acgctgcagc ccaaggcgta
     1141 ccgcgagtcg acgatcctgt tcatcgacgc ggtgcagttc tcgcggctgg cggcgcgcgt
     1201 cgatccggtg agctgtctga agcagctcga cttctacttc tcgctgttcg atcaggtgac
     1261 ggcggcgttc ggggtcgaga aggtcaagac gatcggcgac gcgtacatgg gcgtcgccgg
     1321 ggtgccgcat cgacgggcgg cgcacgcggt cgacgcgacg ctggcggcgt tgcgggtggc
     1381 acgggcgatc gctgcgcgcc cggggccggt ggtcgacgac tgggagtgga gctttcgcct
     1441 cgggctgcac tcgggtccgt gcatcaccgg cgtgctcggg gcgcggcgcc aggtgttcga
     1501 cctgtggggc gacacggtca gcgtggccgc gcaggtccag cgggtcggtc gaccggacac
     1561 ggtatcggtg tcgggcccga cggtcgagct gatcggcggc ttcttcgaca tcggcttcga
     1621 cggcacgacg gtcatccatc acagcggcga gatcgcggtg cacgtggtgc gggggatccg
     1681 tccggagctg ctggcggacg cggaggaggg gatcgtcaac gccgagttct gcaagcgcta
     1741 cgaggcgctg ttcggcgagg cgtgccccga ggccgcgtgg cggccgcagg cggcctcggc
     1801 ggcgctgctc ggcgcgctgt gaaggccaga gccgagtctc caaaactaat tcctcgtagt
     1861 tattgctcga aacagtttca gtaagacacc ttacataaaa gtcagtcgtc accgaatcta
     1921 tggtattggt aatgcccggg gttcgttgac ctggtgctgc cgcgcgtgag agcgtcgggc
     1981 gatgaagccc ggtgcattcg acgagtcatc gaccgcgcac ccggtagcga tcagcgagga
     2041 cctgctggcg agcccgcccg cggtgttccc gggccagctg ctcggggacc gctaccacgt
     2101 cggcgagcgg atcggcggcg gaggcatggc ggacgtgttt cgcgcgcgcg acccgtacct
     2161 gtaccgcgac gtggcgatca aggtcatcaa gcccgggatg gcctcgggcg agatgtgtgg
     2221 ccgcatgttg caggagggtc gggcgaccgc ggcgatcgac catccacact tgctgcgcgt
     2281 gctggacttc gggcgcgtgg gcgcgacggt gtacctcgtc accgacctgc tgcgcggctg
     2341 ctcgctggcg gagcacctgc gttcgctcgc ggacgcgcgg ctcggctggc gggaggcaat
     2401 tgagctgcta ttgcccgcgc tcgacgccct ggcccagatc cacgatcgcg gctttgtcca
     2461 ccgcgacctc aagcccgaca acctgttcgt gcaccatcgc gacggtcgcg aggcgctcat
     2521 cgtcctggac ctcgggatcg caaaggtctc gccggctttc agaggcggcg gcgccctgaa
     2581 caccaccgag tccgggcgag tgctggggac cccggcgtat atgtcgcccg agcaggcggg
     2641 cggcaagccg ctcgatcacc gcaccgatgt ctactcgatc ggcgtgaccc tgtaccgcat
     2701 gctcgccggt cgactgccct tcgaggcgcg cgcgggcgag ggcccgatcg tgttgatggc
     2761 gcatcacctg tacgatcccc cgccgcgcct cgacgaggtc gttccggggc tcccggcgcc
     2821 gctcgtcgag gtggtgatgc ggtcgctggc caaggccccc gccgagcgcc cgcagacgat
     2881 gcgagcgctc gcccaggcgc tccgcgaggc gctcccggcc gagccagccg gcgacgccgc
     2941 ggcgccgacc gtcgcgcgcg tcaagcggac ccggagcgtc ggatggttgt ccggcggcgt
     3001 cgtgttgctg gccgcgctca tcctcctgcg cggcgtccag gaggtcgccg cgacgcccgc
     3061 ggtggccggc tcgccgctcg ctgacgacga tggtggagcg gacgaagcag aggaaatgga
     3121 gatggtgcgg ccgagctcgc cgtcgttcac tctggcgctg ctgcccgatc gacctccggt
     3181 cgccagggcg ccgtccgtgg cgctgccgga gcgacggggg tccgcgcggc gcaagtcgcc
     3241 gcatgccggg gtcgcccggg tcctcgccgg ggtcgccgat ggcgtcggag attgcatcga
     3301 gcgacgcggc gggctcgacc aggagacgct ccaggtgaca tgggacgtgc ggcccgacgg
     3361 tcgcgtgatc gccagcaggc tgccagtggg cacgcgaacg acgctcgcgg ggtgcgttca
     3421 agcgctggtg agcggcttgc agttcccgcc cggcacgggg ctgcacgtcg agcacacgtt
     3481 caagcgggcg ccggaagcga ggcgtccgtg agcgcggcgg cgttgctggt gtggctgacg
     3541 gcgtggggcc ctgcggcccc ggaggtcccg gcggaggcgc gggcgcacga tgaccggggt
     3601 ctcgcataca tcgagggcgg ggacgccgtg gccggtgtgg cggagctcga gcgggcctac
     3661 gcgctgatgc cggacccgct gcggtaccgc gtcggtcgca gcgaggtgct cggttcactg
     3721 cgtggcgcgc tgaccgacct ctacgaggcg accggagacg ccgggcacct gcgacgcctg
     3781 catgtgcatc tgctgcgtca cctcgaggcg ttgatcgtcg cgctgggtga cggcgtcacg
     3841 gcggacgacg tcgcgggcac cctcgtggcc gtgcgcaagg tggagcagca gctcgcccgc
     3901 gtgccgcagg tgatcgccgc gccgccgcgg ccggtaccac gtccgcggtc ggtcgtgccg
     3961 cgtccggcgc ccgcgatgcc cgcggccggg gaggggccga cccgcaggca gctgtacatc
     4021 gccggtggtg tcagcgtcgg catcggcgcc gtgctgctcg gcgtgatggc gtacggcatc
     4081 gcgcaacagc agggtcacgg ggccgccggc tgggcgctca aggacaggat cgacggtcgt
     4141 tcgatcaccc cggccgagcg cgccgagctg gtcggtcacc tcgagggggc gcacgcgaac
     4201 cgacgtctcg cgctcgccac ggggctgtcc tcggccggtg tgtcggtcct cggcgtggcg
     4261 ctgctggccc acggccgcag gcgacccgac gcccgcgagc gcaagaccgg cgtctcgctc
     4321 gccccgtggt gggtgcaagc gggcgtcggc gtcacgctgc tcctgccgct gcgctgatct
     4381 gtcccgagag acacccaaac cgaaggaccc ccatgccacc gctgccccat ctccgcgcgc
     4441 tcctccccat gctcctgtgg acctcgtgct ccgcttcgcc ggtcgtcgac gcgccggcga
     4501 ccgacggcac gaccggccac gaaccgacca gcgctgaccc gcccggcatg acgccgacga
     4561 gcagcgccca gcccacgacc gccgcgagca gcagcacgag cagcggcagc acggcggcgg
     4621 acacgacgac gacgaccacg gagccacaag ccgacacgac cgggaccacc ggcgcaccgg
     4681 ccggctgcgg cgacggtgag gtcgggcccg gagaggcgtg cgacgacggg ctgatgaaca
     4741 gcgacgaggg cgcctgcacc ctgcactgcc aggaaaacac gtgcggcgac ggtctcgtgc
     4801 acgtgggggt cgaggcgtgc gaccacgggg tcgacaacag tgattttgtg tacaacgggt
     4861 gcagcaccca atgcgccaga acaatgttct gcggcgacgg ccaggtcaac ggacccgagg
     4921 agtgcgaccg gggcgagaag aacggcaccg gcgacaagga cgcggacgcg gtgccgtgtt
     4981 cggtgagctg caacttcgag tcgttgctgg tgttcttgag cagccgcacg tacaccgtcg
     5041 cggagctggg cggcagcgcg gcggcggcag acgagcgctg cgaggagctc gcggcggcgg
     5101 cgatgctccc gaaccacatg aagtttcgtg
//
LOCUS       DASWNJ010001635         5899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_340476, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001635 DASWNJ010000000
VERSION     DASWNJ010001635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340476"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2535..2903
                     /locus_tag="VGB85_27170"
     CDS             2535..2903
                     /locus_tag="VGB85_27170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137808.1"
                     /translation="MQHVGDLVLDDLPGLNSLAGLHGLQSAGTISIGDCPPNGGLDGL
                     PDLTGLGGLTSLGTLALFDNEALASFAGLDALTELGGLTALDNPQLSPAVIDAFLAQF
                     DPPPGTCIDCGVCLDDDGSH"
     gene            complement(4087..4521)
                     /locus_tag="VGB85_27175"
     CDS             complement(4087..4521)
                     /locus_tag="VGB85_27175"
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0137809.1"
                     /translation="MRRVQRTDVRLGWGLAWSRSGVYFEGIGSERGIVWRVADSLSLR
                     RFLRLSLEQPTPDHSTLSRTPRRLPAKLHERQRGQDRDKIGIGSGQDPGTSRPRIGPS
                     AGQYARHARQPHGPDPVDFSRCLGAHRTWPSDQVSVAADPCH"
     gene            complement(4518..4769)
                     /locus_tag="VGB85_27180"
     CDS             complement(4518..4769)
                     /locus_tag="VGB85_27180"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0137810.1"
                     /translation="MRSGRLFEGELRRLRTDRIDLLYQHCVDPNVPSEDVAGAVKQLI
                     AEGKIGHFEGPDHVILLAGQRAAQRRAERVFSLDPRPRA"
     gene            5073..5288
                     /locus_tag="VGB85_27185"
     CDS             5073..5288
                     /locus_tag="VGB85_27185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137811.1"
                     /translation="MSSELGAPIQAKLEVGAVNDPLEHEADAIAERVVSELQGPTSLP
                     PTSAPEIVQRKCDACRGRISSPGSGSI"
     gene            5294..>5899
                     /locus_tag="VGB85_27190"
     CDS             5294..>5899
                     /locus_tag="VGB85_27190"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEY0137812.1"
                     /translation="MRIHADARAGESARAVNAQAYTVGQDIVFGQGRYAPGKREGKKL
                     LAHELAHVAQQRSARSGAVLWRRSDGLEPPKEEPKEEPKNPMKNRPGCTYDQAVEIND
                     AVKEADRLLGVVLPKLKDPPAAEVKDALFLAFLADDTATMTTADGVLKKAQARLPSAT
                     IECEQPDGLDYNHPCANLAAYVRWPFAMVGLGRINLCMGNGR"
BASE COUNT          985 a         2094 c         1871 g          949 t
ORIGIN      
        1 cacggttgcg cggtgcggag tctcaggccc gtccgcttcc ccgtcagcca gacagccgca
       61 cacgtgcggg tccggctgac cctgcggtag cgtgtgcctc gccaagcccc gagttgcgcg
      121 cgacgtcgcg caacctggcc cagaggacag ggccgcgggg cgccgcgagg ggggccgaag
      181 tcgaccggat ccggccccgc ggctgtcgac cctgcccccc gtacgagccc ccggacgggc
      241 acatgcgcgg cggcgagatc cccgggtctc ggcccgatcc tgtcccgatt ttgtcgcgat
      301 ccgggtctgt ctcgagacaa agcctcgaat tcgggctcgt agggccccct acgcgaccac
      361 acccatcaca tcaccaacgt cccgtgtgat cgctgttcaa gcagtgagct acgcggctaa
      421 atctgcgttc tgagtggaat ggcgttccag ttctgcgagt accgctccac ggcgccgcga
      481 ggacctgcct cgcagcagga gctcgcgccg gtcccgacgt ccgtgcccac tcacggcctc
      541 gaccccgtcg tcatcagccg cgcatcttag cgaaggaacc tgtggaccct cggcccctcc
      601 ggtactcgac ccgcacgcga gattcccgaa tgaaagtgct tgagcgcagt tcccggccgc
      661 attggtcgga gccgaggtga cgtgtcgctg cgcgagctta gttgactagt agtatccgtc
      721 agcacaggcg gctgcgacag cgaccgtctc atcgtgacat ggcagatcct cgggtacgtc
      781 gcaacttgaa gagaaataat gaatcatgtt gatgtgtcca tgggtgacga acgtgcgcca
      841 tatattgaac tgctcgcaac tgttcgcagc aaggtccgcg gcacacgaca gcatttgcag
      901 gcgctcttca caacctcctt cgggcgcgga cgataccgaa agaagacaca aggtggtcgc
      961 gccaccttcg gaatcttcct gcgaccaggg attgggctcg tagcagcaag acacagccct
     1021 gagataattc gtacagacac tattattgtc cgactccacc ggatcgggct tggtacacgc
     1081 tccggcctgt acctcgacct ccggtagatc ctctggcact ggttgaagtg gggcgtcacc
     1141 cccggtggtg tccacacagc ctgatgagcc cgcggactcc ccgtttgagt cggccgaggc
     1201 tgtgtccgaa ccagttgcct tgccggtcgt cggggggcta gaggtcgttg ccgcgctcgt
     1261 gccctgcgcg ccggtttggc cggcatcatc cgtcaatccg gcggatacag aggagtcgtg
     1321 gggtgcctca ggtccgcacg caaggacgag cgccggcagg gccaaccaag cataagatcg
     1381 catcactctc atcatgccag aacgcttccg ggcgtggggg gccttggcct gctcggcggg
     1441 cgccgacggc gcaggtggcg gagcgctccg gctcgccgag cttctcctca ggcgatggtc
     1501 gagttgctcc caggcacctt gagcgagcac tgcgctaggc ttgtgcgcgc gaatatccac
     1561 cgatgcgata ccattgcgtc cggaggtggc gatgacccgt agaccgcgcg cttggacccc
     1621 attgctcgcc gcatggctcg ccgcctgcgc cgaccgcccg cttccggccg acaccgacgc
     1681 ggcgacgtcc acgaccgaac cggccgacac cggcgaacca ccgcccgtcc cgacgacgac
     1741 cgcagatcct ccgcccacca cgaccacgac gaccgcagat cctccgccca ccacgaccac
     1801 gacgaccacc gaaccgcccg accccacgac cggcagcacc gacgacaccg gctcgaccgg
     1861 tgccccgtcc ccgccgctga ccccgtgcgg ggcctgcccg gtaacctggg agtcccccct
     1921 cgagatcgag atcacgcccg ccaccgacgt cgccgcattt gactgcctga gcgtcatcca
     1981 cggctccctg tcgatctccg gcgacctgac ggcggcgcag ctggcgggcc tcgccaacct
     2041 cacccgggtc gagggcgggc tgtccatcca cggcaacgcc gtgctgaccg acctctcgcc
     2101 gttcgcctgc ctgcgcgaga ccgacggcct gtcgctgtcc ggcctccccg cgctcaccga
     2161 tgccacgggg ctgtccagcc tcgaggagtg cgccggcatc ctacagctcg atgcgacgag
     2221 cctcgcggcg ctgccggcga tggcgccgaa cttccacggc gtcgagtggt tgcagttgag
     2281 cgcgaacccc atcctgatcg acctcacacc ggccgccggc tggacgccgg atccggacct
     2341 gttcctcctg gcggtcgtca acaacgctgc gctgacctcc ccgacggcct gcagccgttc
     2401 gcggcgatcg ccgacgatat ctggatcttg gactcgcccg cactcaccga cctcaccggc
     2461 ctcgagacgc tatccgacaa caacctcagg ctctcgggtc cccgcaactc acgaccctgc
     2521 tcccgctggc gagcgtgcaa cacgtcggcg acctcgtgct cgacgacctc cccgggttga
     2581 actcgctcgc cggtctgcac ggccttcagt cggcgggcac gatcagcatc ggcgactgcc
     2641 ccccgaacgg cggcctggac gggctccccg acctcactgg gctcggcggc ctcacgtccc
     2701 tcggcacctt ggcgctgttc gacaacgaag ccctcgcctc gttcgccggc cttgacgcgc
     2761 tgacagagct gggcggcttg actgctctgg ataacccgca gctgtcaccc gccgtgattg
     2821 acgcgttcct cgcccagttc gacccgcccc cgggcacctg catcgactgt ggagtctgcc
     2881 tcgacgacga cggatcgcac tgacacacgc cgacccggcg cgacctacca gtctcgagca
     2941 ggaccgggcc attcacgtgg gcctcacggg ccaacatgta gcgtctgcgc aggagctcgc
     3001 gctggccccg gtgcaagccg cactcgaccg ctcgccgccc gtgcgctcgg tacggacggt
     3061 tcgtcggcgt aggctgggca tgaactaaag cttccttcga tctaccgcga ccgcgctcgg
     3121 catcctcgtt cgctactgcc ggctccgcac gccaaccact gcgatagttg caacccgccg
     3181 gtcccgcata gcgtcgcgct ggcgggagag acagtggccc tcgacggtgt tctcgcgcgt
     3241 gctcgacacc acgccacctg gtccgaaggc caggtccgcc gggcaccgag acagcagcct
     3301 aaatcgaccg gaaccggccc cacggctgtc gaccctgccc cccgtacgag ccccggcacg
     3361 ggcacatgcg cggcggcgag atccccgggt cttggcccgg tcctgtcccg attttgtcgc
     3421 gatcatggcc tggctcggga caaccctccg aattcgggct cgtagggccc ttcaggcgac
     3481 cacacgcacc acatcaccaa tcatcgaact gaaccgcgtt acagcgcctc ccaacgggcc
     3541 aatacagccc accaagtgga acctggccat ttcatatgtt cgagaggcca tgtggcccct
     3601 cgttggggag cggagcacgg aacgcccgga ggccgcggat cacgtgctcg tgagggactc
     3661 tcgctctgct tccacaaggt ggtcgatgaa taggcgtacc ttgggcgcca ggtgcttgcg
     3721 ggtcggatag atggcgacga tgggcgttgc gcagtcttcg tgagcctcca gcacggcgct
     3781 cgcgggcatc accgcctcgc tcgcccacga gctcgcggag ctcggcgtgc ggcccctcca
     3841 tgccgaagat gtggacaccg caggccgtcg agagcaccac gccgcagccc ggcacgaccg
     3901 cgatcgctgc gtcctgctcg cgctccgcga gcatgatgct gtgcttgcgg accaactgtg
     3961 gtccgtcggg gacatcacga gagcaacgaa gtgccgccac ggcctgtttc taccatgcgg
     4021 tcggccgcgc acgtgggcgc acgacgagcc ggcgcctcct tctggcgcca tgagtcggcg
     4081 tcgatgtcaa tgacagggat ccgcagcgac ggacacctgg tccgaaggcc aggtccgatg
     4141 ggcaccgaga cagcggctaa agtcgaccgg atccggccca tgtggctgcc gagcatgcct
     4201 ggcgtactgc cccgcggacg gccctatgcg cggccgtgag gtcccggggt cttggcccga
     4261 tcctatcccg attttgtcgc gatcctggcc tcgctggcgc tcgtgcagct tggcgggcag
     4321 cctgcgcggc gtccgcgaga gcgtcgagtg gtccggcgtc ggctgctcga ggctgagacg
     4381 aagaaaccga cgcaggctca gcgagtcggc tacccgccat acgatgcccc gctcgctgcc
     4441 tatcccctcg aagtagacgc cggagcgaga ccaggcgaga ccccatccca gtcgaacgtc
     4501 ggtgcgctgc actcgcctca tgctcggggt cgcgggtcca gactgaatac tcgttctgca
     4561 cggcgctgcg ccgctcgctg tcctgcgagg aggatgacgt ggtcgggccc ttcgaagtgt
     4621 ccgatcttac cctcggcgat cagctgcttg acggcgccgg cgacgtcctc gctgggcacg
     4681 ttcgggtcga cgcaatgctg gtacagcagg tcgatgcgat cggtcctgag ccgtcgcagc
     4741 tcgccctcga acagccggcc cgagcgcatc cgcgaggtcg tcgaggccga tccggccggc
     4801 aggcaagagc tgagggcgat aaggttgctt gacagccgga cgacccaaat ctaccgtcgc
     4861 tactggtgac cgtgccgagt gctttgttgc cggcgcgacg gtggttcgcc tgtattacga
     4921 atccattcgt aacgagttct ggagttctca tgacgtacgc accgctcaaa cgccccgaac
     4981 gtaccgcagc cctgccaagg ccgtccaaag tcaccaatcg acgcccgccc cgaatcagcg
     5041 gaccatccgt cgatagccgt cgcctatcca gtatgtcgag cgagctcggc gcaccgattc
     5101 aggccaagct ggaggtcggt gccgtcaacg acccgctcga gcacgaagcc gacgcaatcg
     5161 ccgagcgcgt cgtttcggag ttgcaggggc ccacttcgct ccctcctacg agcgcccccg
     5221 agatcgtcca gcgcaagtgc gacgcgtgcc gcgggcgtat ttcgagcccc ggttcgggtt
     5281 cgatctgagc cgggtgcgga ttcacgcgga cgcgcgggca ggagaatcgg cgcgggcggt
     5341 gaacgcgcaa gcatatacgg tggggcagga catcgtgttc gggcaggggc ggtatgcgcc
     5401 ggggaagcgg gaggggaaga agctgctcgc gcacgagctg gcgcatgtgg cgcagcagcg
     5461 ctccgcgcgc tccggagccg tgctgtggag gaggtcggac gggctcgagc cgccaaagga
     5521 agagccgaag gaggagccga agaatccgat gaagaatcga ccgggctgca cctacgatca
     5581 ggccgtcgag atcaacgacg ccgtcaagga agcggacagg ctgctcggcg tggtgctccc
     5641 caagctgaag gacccgcccg cggcggaggt gaaagatgcg ctgttcctcg cgttcctcgc
     5701 cgacgacacc gccaccatga cgacagccga tggcgtgctg aagaaggccc aggcgaggct
     5761 gccgtccgcc acgatcgagt gcgagcagcc cgacgggctc gattacaatc acccctgcgc
     5821 taacctcgca gcctacgtgc gctggccctt tgccatggtg gggttggggc gcatcaacct
     5881 ctgcatggga aatggccga
//
LOCUS       DASWNJ010001636         3310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_99835, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001636 DASWNJ010000000
VERSION     DASWNJ010001636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99835"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..290
                     /gene="rplX"
                     /locus_tag="VGB85_27195"
     CDS             3..290
                     /gene="rplX"
                     /locus_tag="VGB85_27195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEY0137813.1"
                     /translation="MVVLTGKDKGKQGEVLKVIPKEDRVVVQGVMMVKRHQKATPQAA
                     GSIVQKEATIHVSNVSHVDPQDGKATRVGFKFLEDGRKVRYAKRSGEVLDR"
     gene            302..844
                     /gene="rplE"
                     /locus_tag="VGB85_27200"
     CDS             302..844
                     /gene="rplE"
                     /locus_tag="VGB85_27200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEY0137814.1"
                     /translation="MPARLHEHYKTVLTPALTEQFGYKNPMQVPRLEKIVLNMGVGEA
                     VADRKKLDAAMGDMTKIAGQKPVATIARKAIANFKLREGMPIGCKVTLRRDRMYEFLD
                     RLLTIALPRVRDFRGCSPKSFDGHGNYALGIKEQIVFPEINYDDIDEVRGMDVIIGTT
                     ALTDEEGKALLEGLGLPFRK"
     gene            860..1165
                     /gene="rpsN"
                     /locus_tag="VGB85_27205"
     CDS             860..1165
                     /gene="rpsN"
                     /locus_tag="VGB85_27205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEY0137815.1"
                     /translation="MAKKSAVEKNEHRKRLVDKFKTKRAELKKVVMDRSIELQDRFDA
                     TLKLAAMPRNGSRTRIRNRCLLTGRPRAYYRKFGLSRIALRDLASEGRIPGVVKSSW"
     gene            1184..1582
                     /gene="rpsH"
                     /locus_tag="VGB85_27210"
     CDS             1184..1582
                     /gene="rpsH"
                     /locus_tag="VGB85_27210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEY0137816.1"
                     /translation="MSMSDPLADMLTRIRNGQMANKPVVTAPSSKLRENVLEVLRREG
                     YIRGFEPLDLGSGKRDVSIQLKYQNGEPVIRELKRVSKPGRRIYAGVRELPQVFNGLG
                     IAILSTPRGVMSDAEAREQHVGGEVLCTVF"
     gene            1585..2121
                     /gene="rplF"
                     /locus_tag="VGB85_27215"
     CDS             1585..2121
                     /gene="rplF"
                     /locus_tag="VGB85_27215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEY0137817.1"
                     /translation="MSRIGKHPVVVPQGVDLAIEGNVVRAKGKLGELRTVLPPEVTVV
                     LEDGNVVVGPRDKEERRARAMWGLSRTLVQNMVTGVATGFSKKLEISGVGYRAAVDGG
                     MLSLQLGYSHDIKYAIPPDVKIVADTPTAVTVSGADRQRVGQIAAEIRRFREPEPYKG
                     KGVKYAGEKIRRKEGKKK"
     gene            2126..2491
                     /gene="rplR"
                     /locus_tag="VGB85_27220"
     CDS             2126..2491
                     /gene="rplR"
                     /locus_tag="VGB85_27220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEY0137818.1"
                     /translation="MVKPIDRLARRRRRVRYALKSNASGDRLRLSVHRSGRHMYGQVV
                     DDEQGRTVAAASTLEKVLREELKSGADKDAARRVGLLLAERAKAAGVAKVMFDRGGHK
                     YHGRVKALADGAREGGLEF"
     gene            2506..3087
                     /gene="rpsE"
                     /locus_tag="VGB85_27225"
     CDS             2506..3087
                     /gene="rpsE"
                     /locus_tag="VGB85_27225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEY0137819.1"
                     /translation="MARDGGGGGNRGGRDDRRDGGDELVDRLVNVNRVAKVVKGGRRF
                     GFSALVVVGDGKGRVGYGSGKAREVPEAVRKATEQAKRSMVRIPLRQGRTLHHDVQGH
                     FGAGRVLMRSAPPGTGIIAGGPMRAVCEALGIHDVVAKSLGSQNPHNMVKATFAALAA
                     MMSPRAVAAKRGKKVSDLLGRRDSAEGTTGRVE"
     gene            3092..3310
                     /gene="rpmD"
                     /locus_tag="VGB85_27230"
     CDS             3092..3310
                     /gene="rpmD"
                     /locus_tag="VGB85_27230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEY0137820.1"
                     /translation="MTDRLKITQTGSPIGRPATQRKTLIALGLNKLQRSRVVYATPSM
                     QGQIDKVKHLLKVEPAAAEQEQAQGGEG"
BASE COUNT          595 a         1090 c         1202 g          423 t
ORIGIN      
        1 gcgtggtcgt gctcaccggc aaggacaagg gcaagcaggg cgaggtgctg aaagtcatcc
       61 ccaaggagga ccgcgtggtc gtccagggcg tgatgatggt caagcgccac cagaaggcca
      121 ccccgcaggc tgccggcagc atcgtccaga aagaggcgac gatccacgtc tccaatgtct
      181 cgcacgtcga tccccaggac ggcaaggcga cccgggtcgg tttcaagttc ctcgaggacg
      241 gccgcaaggt ccgctacgcc aagcggtccg gcgaagtcct cgaccgctga aggtacgggc
      301 catgcctgcc aggctccacg agcactacaa gacggtcctc acgccggcgc tgaccgagca
      361 gttcgggtac aagaacccga tgcaggtgcc gcggctggag aagatcgtcc tcaacatggg
      421 cgtgggcgag gcggtcgccg accgcaagaa gctcgacgcc gcgatgggcg acatgaccaa
      481 gatcgccggc cagaagccgg tggccacgat cgcccgcaag gcgatcgcga acttcaagct
      541 ccgggaaggc atgccgatcg gctgcaaggt caccttgcgc cgcgaccgga tgtacgagtt
      601 cctcgaccgg ctgctgacca tcgccttgcc cagggtccgc gatttccgcg ggtgctcgcc
      661 caagagcttc gacgggcacg gcaactacgc gctgggcatc aaggagcaga tcgtcttccc
      721 cgagatcaac tacgacgaca tcgacgaggt ccgggggatg gacgtcatca tcggcacgac
      781 ggcgctgacc gacgaggagg gcaaggccct tctcgaaggc ctcggcctgc cgttcaggaa
      841 gtagcgggac cggactgaca tggctaagaa gagcgccgtc gagaagaacg agcaccgcaa
      901 gcggctcgtc gacaagttca agacgaagcg ggcggagctg aagaaggtcg tcatggaccg
      961 ctcgatcgag ctccaggacc gcttcgacgc gaccctgaag ctggcggcga tgccccgcaa
     1021 cggcagccgg acgcggatcc gcaaccgctg cttgctcacc ggccggccgc gcgcctacta
     1081 ccgcaagttc ggcctgtcgc ggatcgcgct cagggatctg gcctccgaag gccggatccc
     1141 gggcgtggtg aagtcgagct ggtagcggcg ggagcacctc ctcatgtcga tgagtgaccc
     1201 gctcgcggac atgctcaccc gcatccgcaa cgggcagatg gccaacaagc cggtggtcac
     1261 ggccccgagc tcgaagctgc gcgagaacgt gctcgaggtg ttgcgccgcg agggctacat
     1321 ccgcggcttc gagccgctcg atctcggcag cggcaagcgt gacgtgtcca tccagctgaa
     1381 gtaccagaac ggcgagccgg tgatccgcga gctcaagcgc gtgtcgaagc cgggccgccg
     1441 gatctacgcc ggcgtgcggg agctgcccca ggtcttcaac ggcctcggca tcgctatcct
     1501 gtccacgccg cgtggcgtaa tgtcggacgc cgaggcgcgc gagcagcacg tcggcggcga
     1561 ggtcctttgc acggtgttct gaccatgtcg cgtatcggca agcatcccgt cgtcgtgccc
     1621 cagggcgtgg acctggcgat cgagggcaac gtcgtccggg ccaagggcaa gctcggcgag
     1681 ctgcgcaccg tcctgccgcc cgaggtcacg gtcgtgctcg aggacggcaa cgtcgtcgtc
     1741 gggccgcgcg acaaggagga gcgtcgcgcc agggcgatgt ggggcctgtc gcgcaccctg
     1801 gtgcagaaca tggtcaccgg cgtggcgacg ggcttctcga agaagctcga gatcagcggc
     1861 gtcggctatc gcgccgcggt cgacggcggc atgctgagcc tccagctcgg ctacagccac
     1921 gacatcaagt acgcgatccc gcccgacgtg aagatcgtcg ccgacacgcc caccgcggtc
     1981 acggtgagcg gcgccgaccg gcagcgggtg ggtcagatcg cggccgagat ccgccggttc
     2041 cgcgagcccg agccctacaa gggcaagggc gtgaagtacg ccggcgagaa gatccgccgc
     2101 aaggaaggca agaagaagta gtgccatggt gaagccgatt gatcgcctcg cccggcgcag
     2161 gcgccgggtg cgctacgccc tcaagtcgaa cgcctccggc gaccggctgc ggctgtccgt
     2221 ccaccgctcc ggccgccaca tgtacggcca ggtggtcgac gacgagcagg gccgcaccgt
     2281 cgccgcggcc tcgacgctgg agaaggtgct gcgcgaggag ctaaagtccg gggcggacaa
     2341 ggacgctgcc cgccgcgtcg gcctgctcct ggccgagcgc gcgaaggcgg ccggcgtcgc
     2401 caaggtcatg ttcgaccgcg gcggacacaa gtaccacggc cgcgtgaagg ccctcgccga
     2461 cggcgcccgc gagggcggcc tcgagttctg agttagggga agaggatggc aagggacgga
     2521 ggcggcggcg gcaaccgcgg cgggcgtgac gaccggcggg acggcggcga cgagctcgtc
     2581 gaccgcctgg tcaacgtcaa ccgcgtcgcg aaggtggtga agggcggtcg ccggttcggc
     2641 ttctcggcac tcgtggtggt gggcgacggc aagggccggg tcggctacgg cagcggcaag
     2701 gcgcgcgagg tgccggaggc ggtccgcaag gccaccgagc aggcgaagcg ctcgatggtg
     2761 cgcatcccgc tgcgccaggg ccggaccctg caccacgacg tgcagggcca cttcggcgcc
     2821 ggccgggtgc tgatgcgctc ggcccccccg ggcaccggca tcatcgccgg cggcccgatg
     2881 cgggcggtgt gcgaggcgct gggcatccac gacgtggtgg ccaagagcct cggctcgcag
     2941 aacccgcaca acatggtgaa ggcgaccttc gcggccctgg ccgcgatgat gtcgccgcgc
     3001 gcggtggcgg cgaagcgcgg caagaaggtc tcggacctgc tcgggcgccg cgacagcgcc
     3061 gagggcacca ccggcagggt ggagtagggt cgtgaccgac aggctgaaga tcacccagac
     3121 cgggagcccg atcggccggc cggcgacgca gcgcaagacg ctgatcgccc tcgggctgaa
     3181 caagctgcag cgctcgcggg tcgtctacgc gacgccctcg atgcagggcc agatcgacaa
     3241 ggtgaagcac ctcctcaagg tcgagccggc tgcggcggag caggagcagg cccaaggggg
     3301 cgagggatga
//
LOCUS       DASWNJ010001637         2184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_698855, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001637 DASWNJ010000000
VERSION     DASWNJ010001637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698855"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VGB85_27235"
     CDS             <1..741
                     /locus_tag="VGB85_27235"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HEY0137821.1"
                     /translation="RREASTTSAALLRIAVVLLLWTRWAADLTFVATPSVALSLLFFI
                     ASSCLLIGFCTRVAAVATAIAVSILYFWFGAHLGRVAWIHHHHYLLVSAAWICALTPC
                     GRSLAIDARGGSGRGPVWALRLYTLQLAAVYLWSGVDKLSPAFLSGARLEQLAMDLYF
                     GSDPPQWHLLAVAGAWLVMVLELALGLGFLLGKTPRWLLLAGIGLHATIYLCMPVATF
                     SATMVCLYLAALDPEVVHRTLHARAPSA"
     gene            826..1551
                     /locus_tag="VGB85_27240"
     CDS             826..1551
                     /locus_tag="VGB85_27240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137822.1"
                     /translation="MGVTACGPAKGTPAETGSTSGAAESSSSTEQPTGGGTSSTGGST
                     AGPSASSSTGEGTSGESTTGCTTGETVECMGIAVTPGGALGCDGDELALDDDCDALAG
                     YEVCADGTVHRHTSVPCNTNEDIMACWGPYYGCSSDEDCMEGLACECELRWAGDGPHG
                     PGLPYTFNRCVAADCRSDADCGGLRCGVSDDGCGGIGGFFCRTPEDDCSSDLDCPDYD
                     VGNRCAYSWELERWACDHYYFCE"
     gene            complement(1591..>2184)
                     /locus_tag="VGB85_27245"
     CDS             complement(1591..>2184)
                     /locus_tag="VGB85_27245"
                     /inference="COORDINATES: protein motif:HMM:NF036933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HEY0137823.1"
                     /translation="NPANPETSLWQLYGSEDGTTVTIVADAQVTGLPPNPIKLDKGKV
                     VEFYAGGTMTEPGDFEITADKPIAVMNYMTGAENMPAPFNSTGDPLSVQIPAVEQYLP
                     RYVVLVPGTWTNDVGVFTRMAGAVITIDGVQIPDTAFNPVGNSGFEVARVPLLDGVHV
                     LEGGETPFSVIVVGYDQHDSYGYLGGTGTKIINPAPQ"
BASE COUNT          279 a          678 c          847 g          380 t
ORIGIN      
        1 cgccgcgagg cctcgaccac cagcgccgcg ctgctgcgga tcgcggtcgt gctgctgctg
       61 tggacccgct gggccgcgga cctgaccttc gtggcgacgc cgagcgtcgc gctgtcgctg
      121 ctgttcttca tagccagcag ctgcctgctg atcgggtttt gtacccgcgt cgccgcggtg
      181 gccacggcga tcgcggtgtc gatcctgtac ttctggttcg gtgcgcacct cgggcgcgtc
      241 gcgtggatcc accaccatca ttatctgctg gtcagcgcgg cgtggatctg cgcgctgacg
      301 ccgtgcgggc ggtcgctcgc gatcgacgcg cggggcgggt ccgggcgcgg gccggtgtgg
      361 gcgctgcgcc tgtacacgct ccagctcgcg gcggtgtacc tgtggagcgg ggtcgacaag
      421 ctctcaccgg cgttcctctc gggggcccgg ctcgagcagc tggcgatgga cctgtacttc
      481 gggtccgacc cgccgcagtg gcacctgctg gcggtcgccg gcgcgtggct cgtcatggtg
      541 ctcgagctgg cgctcgggct cggcttcttg ctgggcaaga ccccccgctg gctgttgctc
      601 gcgggcattg gtctccacgc gacgatctat ctatgcatgc cggtggcgac cttctcggcg
      661 acgatggtgt gcctgtatct cgcggcgctc gacccggagg tcgtgcatcg caccttgcat
      721 gccagggcgc cgtcggcctg atcacgacgc ccggcacgga gctgcgatcc ggcatacgct
      781 gtgcgggtgc ggtcacggca atttttgtgg gtgggctcgg tgcgggtggg agtcacggcg
      841 tgcgggcccg cgaagggcac gcccgcggag acgggctcga cgagcggtgc ggcggagtcg
      901 agcagctcga cggagcagcc cacgggcggc ggcacgagca gcacgggtgg gagcaccgcg
      961 gggcccagcg cgtcgagcag cacgggtgag ggcacgtcgg gtgagagcac gacggggtgc
     1021 acgaccggcg agaccgtcga atgcatgggc atcgcggtga cgccgggcgg ggcgctcggt
     1081 tgtgacgggg acgagctcgc gctcgacgac gactgtgatg cgctcgcggg gtacgaggtc
     1141 tgcgcggacg gtacagtcca tcgtcacacg agcgtgccgt gcaatacgaa cgaggatatc
     1201 atggcgtgct gggggccgta ctacggttgt tccagcgacg aggattgcat ggagggtctg
     1261 gcttgtgaat gcgagctgcg ctgggccggc gacgggccgc acgggccggg gctgccgtat
     1321 acgttcaatc gctgcgtcgc cgcggattgc cgcagcgacg ccgactgcgg cggcctgcgc
     1381 tgcggcgtgt cggacgacgg ctgcggcggc atcggcggct tcttttgccg caccccggag
     1441 gacgactgca gcagcgatct ggactgcccg gactacgacg tcggcaatcg ctgcgcgtac
     1501 tcgtgggagc tcgagcgctg ggcctgcgac cactactatt tttgcgaatg aggagacccg
     1561 cggccggggc cgcgggtcgt catgcccgca tcactgcggg gcggggttga tgatcttcgt
     1621 gccggtgccg ccgaggtagc cgtagctgtc gtgctggtcg tagccgacga cgatcacgct
     1681 aaacggggtc tcgccgccct cgagcacgtg gacgccgtcg agcaggggga cgcgggcgac
     1741 ctcgaagccg ctgttgccga ccggattgaa ggcggtgtcg gggatctgga ccccgtcgat
     1801 cgtgatcacg gcgccggcca tgcgggtgaa cacgccgacg tcgttggtcc aggtgccggg
     1861 cacgagcacg acgtagcgcg gcaggtactg ctcgacggcg gggatctgga ccgagagcgg
     1921 gtcgccggtg ctgttgaagg gcgccggcat gttctcggcg ccggtcatgt agttcatgac
     1981 ggcgatcggc ttgtcggcgg tgatctcgaa gtcgcccggc tcggtcatgg tgccgccggc
     2041 gtagaactcg acgaccttgc ccttgtcgag cttgatcggg ttgggcggca ggccggtgac
     2101 ctgggcgtcg gcgacgatcg tgacggtggt gccgtcctcg gacccgtaga gctgccacag
     2161 cgaggtctcg gggttggcgg ggtt
//
LOCUS       DASWNJ010001638         4105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_561004, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001638 DASWNJ010000000
VERSION     DASWNJ010001638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561004"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2863
                     /locus_tag="VGB85_27250"
     CDS             <1..2863
                     /locus_tag="VGB85_27250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137824.1"
                     /translation="RLEDELATDLPGDRGWTLRRLADRCDALQARAFARKLDALLQDL
                     LHDAIGVAPPTLTPAWRDALRFYLGASDRNRALLRELLRFAAAHPGRSIAPTLPRNHA
                     WLARAAAHMRTDAWLAPQQRTLEIAGLRHDLRIEDDPLEVLRMGIPFGTCLALDTGFN
                     ADSTVLNAVDINKRVLYLRDPRGSVIARQLIAVSSDFGLLGYRLYCARGLEDRPAVTA
                     AFQALCSDVAAAAGLPFTDGGAPETIHPGFWYDDGAESWTRPGRSRPDLEPYFAALGR
                     PVPDRLGEWALDRPRAWQVLQDDDHEAGLALLANLRNDAAALPVIRMVAARIPARELA
                     RRARRDRVLARVLVVSQLDHGPAAAMRAATGVRHDRDLWSELASLHEHSLPSAELARA
                     WLDAAAIERRDCQFFDDHGLGHRTVDMGDSLACLPIAELLTRCHELTALWDWIVAEQD
                     HCADCRERGLDGLRRAALASHARAPDPDPIVTALRGRHGPLAQDVALHVAARFSLASR
                     PCPLEHGLGASWLTRLGQRPLPAPHALRAVRELRRARPDLADTPDMFAALVRQAGPRA
                     QHDDLPRPTTAPFAALGELLLHLPDPAALLAAWHEPAERPVKWQPDPFEAHFHRTVPT
                     PWRRHLARRVADDTEAALWLARLGDVDALTRLERRSYLALAQDIRRQHALRDRPADVP
                     CDMSLLTSMLAARGDLRPGDRHMIDLALLREAARALAPPHDPATLEPALALLRLAGLS
                     ADVWQDIIARLLPPAGPPAPELVPLLADLLAHEDPRTLPTAQIARLAAAPALHRPLID
                     HLERGLRVHRVDIHHFYSQFERAPDLDPATRDAFLAAWISSCGADAADIWTPLGFAED
                     QRFAACLPIWLQHHPPTWLAIYRELRELHFQALFLDALQTLAPTRAADLAELHQLATS
                     TWDPHGDEEILARQWLLAAMPAGISTELSRETNDLSLPD"
     gene            complement(2849..3757)
                     /locus_tag="VGB85_27255"
     CDS             complement(2849..3757)
                     /locus_tag="VGB85_27255"
                     /inference="COORDINATES: protein motif:HMM:NF025625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY0137825.1"
                     /translation="MLPVPPRSIRLFLVDGTPSGLRTAEIGLSTCKAVACPRASLGGL
                     RTRPEANRTGVYVLIGEEHPDHPGRTAVYVGEGDEVIERIVRHENSKDFWDRVVIFVS
                     KDENLTKAHVRNLEARLVALAREANRAFVVNRNDPEGGRLPEADEAEMTQFLEQIRTL
                     LGIFGISEFEPVRTRLATPVIAGNPAQNVIDSRPTFFLSVGGTEAQGVLDEAGFIVRK
                     GSHARKIAKSSIKSYRQANREELVSSGVLKDAGSDLQFTQDFLFTSSSAAASVVSGTN
                     MNGRVVWKLADGTTLKMWEERVLVGK"
     gene            3841..>4105
                     /locus_tag="VGB85_27260"
     CDS             3841..>4105
                     /locus_tag="VGB85_27260"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0137826.1"
                     /translation="MQVREIHRGRLIDHIHLVVADLERSKRFYAAVLGALGLGLGGEG
                     PDFFWADELFVSTPTSPAAAGEPTGRVHLAFQASDRAAVEAFHR"
BASE COUNT          576 a         1699 c         1209 g          621 t
ORIGIN      
        1 caggctcgag gacgagctcg cgaccgacct gcccggcgac cgcggctgga ccctgcgccg
       61 cctcgccgac cgctgtgacg cgctgcaggc ccgcgccttc gcccgcaagc tcgacgcgct
      121 gctgcaggac ctcctgcacg acgcgatcgg cgtcgccccg ccgacgctga cgcccgcctg
      181 gcgcgacgcg ctgcggtttt acctcggcgc ctcggaccgc aaccgggccc tgctgcgcga
      241 gctgctgcgc ttcgccgccg cccaccccgg ccgatcgatc gccccgaccc tgccgcgcaa
      301 ccacgcctgg ctcgcccgcg ccgccgccca catgcgcacg gacgcctggc tcgccccgca
      361 gcaacgcacc ctcgagatcg ccggtctccg tcacgacctg cgcatcgagg acgacccgct
      421 cgaggtcctg cgcatgggga tcccgttcgg cacctgcctc gcgctcgaca ccggcttcaa
      481 cgccgactcg accgtgctca acgccgtcga catcaacaag cgcgtgctct acctccgcga
      541 cccgcgcggc agcgtgatcg cccgccagct gatcgccgtc tcgagcgact ttggcctgct
      601 cggctaccgc ctctactgcg cgcgcggcct cgaggaccgc cccgccgtca ccgccgcctt
      661 ccaggccctg tgcagcgacg tcgccgcggc cgccggcctg cccttcaccg acggcggcgc
      721 cccggagacg atccaccccg gcttttggta cgacgacggc gccgagtcct ggacccgtcc
      781 cggccgcagc cgccccgacc tcgagcccta cttcgccgcc ctcggtcgac ccgtgccgga
      841 tcggctcggc gagtgggccc tcgaccgccc gcgcgcctgg caggtcctcc aggacgacga
      901 ccacgaggcc ggcctcgccc tgctcgccaa cctgcgcaac gacgccgccg cgctccccgt
      961 gatccgcatg gtcgccgcgc ggatccccgc gcgcgagctg gcccgacgcg cccgccgcga
     1021 ccgcgtgctc gcgcgcgtcc tcgtcgtgtc gcagctcgac cacggccccg ccgcggccat
     1081 gcgcgccgcg accggcgttc gccacgaccg cgacctgtgg agcgagctcg ccagcctgca
     1141 cgagcacagc ctgccctccg ccgagctcgc gcgcgcgtgg ctcgacgccg ccgcgatcga
     1201 gcgccgcgac tgccagttct tcgacgacca cggcctcggc catcgcaccg tggatatggg
     1261 cgactcgctc gcctgcctgc cgatcgccga gctgctgacc cgctgccacg agctcaccgc
     1321 gctgtgggac tggatcgtcg ccgagcagga ccactgcgcc gactgccgcg agcgtggcct
     1381 cgacggcctg cgccgcgccg ccctcgcgag ccacgcccgc gcccccgacc ccgacccgat
     1441 cgtcaccgcc ctgcgcggcc gccacggccc gctcgcccag gacgtcgcgc tgcacgtcgc
     1501 cgcgcgcttc tccctggcct cccgaccatg tcctctggaa catggcctcg gggccagctg
     1561 gttgacccgc ctcggccagc gcccgctgcc cgccccgcac gccctgcgcg ccgtccggga
     1621 gctgcggcgc gcccgcccgg acctcgccga cacgcccgac atgttcgccg cgctggtccg
     1681 ccaggccggc ccccgcgcgc agcacgacga cctcccgcgc ccgaccaccg cccccttcgc
     1741 cgcgctcggc gagctgctgc tccacctgcc cgaccccgcc gcgctgctcg ctgcgtggca
     1801 cgagccggcc gaacgacccg tgaagtggca gccggacccc ttcgaggccc actttcatcg
     1861 cacggtcccg accccgtggc gccgccatct cgcccgtcgc gtcgccgacg acacggaggc
     1921 cgcgctgtgg ctcgcgcgcc tcggcgacgt cgacgccctg acccgcctcg agcgccggtc
     1981 ctacctcgcg ctcgcgcagg acatccgccg ccagcacgcg ctgcgcgacc gccccgcgga
     2041 tgtcccttgc gacatgtccc tcctgacatc catgctcgcg gcccgcggcg acctgcgccc
     2101 cggcgaccgg cacatgatcg acctcgccct gctgcgcgag gccgcccgcg cgctcgcgcc
     2161 tccgcacgac cccgccacgc tggagcccgc gctcgccctg ctgcgcctcg ccggcctgtc
     2221 cgccgacgtc tggcaggaca tcatcgctcg cctgctgccg cccgccggcc cgcccgcgcc
     2281 ggagctcgtg cccctgctcg ccgacctgct cgcccacgag gacccgcgca cattgccgac
     2341 cgcgcagatc gcccgcctcg ccgccgcgcc ggccctgcac cgcccgctca tcgatcacct
     2401 ggagcgcggc ctgcgggtcc accgcgtcga catccaccac ttctacagcc agttcgagcg
     2461 cgcgcccgac ctcgaccccg cgacccgcga cgccttcctc gccgcctgga tcagcagctg
     2521 cggcgccgat gcggccgaca tatggacgcc cctcggcttc gctgaggatc agcgctttgc
     2581 tgcctgcctg ccgatatggt tgcaacacca cccgccgacc tggctggcga tctaccgcga
     2641 gctgcgcgag ctccacttcc aggccctgtt cctcgacgcc ctccagaccc tcgcgcccac
     2701 ccgcgcggcc gacctcgccg agctgcacca gctcgccacc tccacctggg acccccacgg
     2761 cgacgaggag atcctcgccc gccagtggct gctcgccgcc atgcccgccg ggatatccac
     2821 ggagctgtcc cgagagacaa acgacttgtc acttcccgac tagcacacgc tcctcccaca
     2881 ttttcaaagt ggtaccatcg gccagcttcc agacgacacg gccattcatg ttcgtgccag
     2941 ataccaccga agcggccgcg cttgaggacg taaagaggaa atcctgggtg aattgcaggt
     3001 cgcttcctgc gtccttgaga acgccactcg aaacaagctc ttctcgattg gcttgcctgt
     3061 agctcttgat cgatgacttc gcaatcttcc tcgcgtgcga gcctttacga acgatgaaac
     3121 cagcctcatc cagaacgcct tgagcttcgg ttcctccaac gctgagaaag aacgtaggtc
     3181 ttgaatcgat gacgttttga gccggatttc cggcgatcac gggtgtcgca agtcgggtgc
     3241 gcaccggctc gaattcagag atcccgaaaa tccccagaag tgtacgtatc tgctccaaga
     3301 actgagtcat ctcggcctca tcggcctccg gcaaacgccc tccttccgga tcattgcggt
     3361 tgacgacgaa ggcccggttc gcctcgcgtg caagcgccac caatctggcc tcaaggttac
     3421 gcacatgggc cttggtaagg ttttcgtcct tactgacgaa gatgacgact cgatcccaga
     3481 aatcttttga attctcgtga cgtacgatcc gttctatgac ctcgtcaccc tcaccaacat
     3541 acacggcagt tcgtccggga tgatccgggt gttcttctcc tatcagcaca tacacgcctg
     3601 tacgatttgc ttccggtcgt gtgcgcaagc cgccgagcga cgctcgagga caggccacag
     3661 ctttgcatgt ggagaggccg atctccgcgg tgcggagccc agaaggggta ccgtctacaa
     3721 ggaataagcg aatcgagcgc ggcggcacgg gcagcatcct accgcatatc tcgggtaccc
     3781 gctgagcttg acctgtcttg aggcgcgtgc gaaactgctc tcccttccag ggagaccagc
     3841 atgcaggtgc gagagatcca tcgcggacgg ctcatcgatc acatccacct cgtcgtcgcc
     3901 gacctcgagc gcagcaagcg gttctacgcc gcggtgctcg gcgcgctcgg cctcggcctc
     3961 ggcggcgagg gcccggactt cttctgggcc gacgagctgt tcgtctcgac cccgacctcg
     4021 cccgccgcgg ccggtgagcc gaccgggcgc gtgcacctcg cgttccaggc cagtgaccgc
     4081 gccgcggtcg aggccttcca ccgcg
//
LOCUS       DASWNJ010001639         2721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_488507, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001639 DASWNJ010000000
VERSION     DASWNJ010001639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488507"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /locus_tag="VGB85_27265"
     CDS             <1..1312
                     /locus_tag="VGB85_27265"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEY0137827.1"
                     /translation="DEAFAVAWELASDEAFTDIVQSGTADAVSMWAHSLHIEVNDLEP
                     DTWYWYRFKVGTYTSPAGRTRTTPAFDAQPERLRFATASCQDYSDGYYTAYRAMAKED
                     LDLVLFLGDYIYENAATGPVRSHGAPEPMTLDEYRVRHALYKSDRDLQAMHQRCPWAL
                     IWDDHEVENDYAADHSQDDAPIAEWLARRAAAYQAYYEHMPLRLPPPSGPDYKIYHAL
                     KWGDLAELWLLDTRQYRSVQNCMNKPGQGCEGWEEFEGTLLGDEQETWLKEQMAATAA
                     IWKCITQQVVFSTVNFGGSLVNFDQWDGYPKARQRILDFIVDEQLDNVVVLSGDIHLG
                     GLGDLTAIADDDESPVIATEIVTTSITSSANVPPEAIEGALGGLPLLKYVNAHSRGYV
                     SHDVTRAVYTVRCVIVDSIVDPMSPAMVEVERHIDAGVAGYRPV"
     gene            1358..1798
                     /locus_tag="VGB85_27270"
     CDS             1358..1798
                     /locus_tag="VGB85_27270"
                     /inference="COORDINATES: protein motif:HMM:NF024559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3995 domain-containing protein"
                     /protein_id="HEY0137828.1"
                     /translation="MLTVSSLAAAVVAACLIVLAFIHVYWGAGGAWPARTRAGLGPMV
                     VGTPPGTAMPNLPACLLVAALLVTAAGLVASAGLADEPPWFARLGSAGVAGVLALRGL
                     GGFIDGRLRPDTRTQPYYALNRRIYSPLCLALAALIAVVLACRA"
     gene            1795..2349
                     /locus_tag="VGB85_27275"
     CDS             1795..2349
                     /locus_tag="VGB85_27275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137829.1"
                     /translation="MTRMTREDWLDAGLALLASDGRWALTQGRLCARLARTKGSFYHH
                     FADMLGYQRELLARWREQNTEAMIEGSRTAGDPRARGERLREQVAAADLRVERAIRAW
                     AEVEPDARAALDEVDARRLAYLTELAHARVADPARAVVVARLEYAAFIGTVTLFHALP
                     QAQRELLSRSLGEALALWANTTDA"
     gene            complement(2355..>2721)
                     /locus_tag="VGB85_27280"
     CDS             complement(2355..>2721)
                     /locus_tag="VGB85_27280"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY0137830.1"
                     /translation="RHVQIVLSEPDATGVPDQAVLSPRTWRVADLHTKHALLRAGLGW
                     GNLPEALVADDLAGGTLLRIQPDAWGPDEHTLHLSAVYRPDTVLGPAHRWVLAHLAQL
                     CQRDAQAPAAKGRGRARRG"
BASE COUNT          388 a         1039 c          898 g          396 t
ORIGIN      
        1 cgacgaggcc ttcgcggtcg cgtgggagct cgccagcgac gaggccttca ccgacatcgt
       61 ccagtccggc accgccgacg ccgtgtcgat gtgggcccat tcgctgcaca tcgaggtcaa
      121 cgacctcgag cccgacacct ggtactggta tcgcttcaag gtcggtacat ataccagccc
      181 ggccgggcgc acgcgcacga cccccgcgtt cgacgcccag ccggagcgcc tgcgcttcgc
      241 gaccgcctcc tgccaggatt actccgacgg ctactatacc gcgtatcggg cgatggcgaa
      301 ggaggacctc gacctcgtgc tgttcctcgg cgattacatc tacgagaacg ccgcgaccgg
      361 cccggtacgc tcgcacggcg ctccggagcc gatgaccctc gacgagtatc gcgtgcgtca
      421 cgccctgtac aagtccgacc gtgacctcca ggcgatgcac cagcgctgtc cgtgggcgct
      481 gatctgggac gaccacgagg tcgagaacga ctacgccgcc gatcactcgc aggacgacgc
      541 gccgatcgcc gagtggctgg cgcgccgcgc cgccgcctac caggcctact acgagcacat
      601 gccgctgcgc ctgccgcccc cgagcggccc ggactacaag atctaccacg cgctcaagtg
      661 gggcgacctc gccgagctct ggctgctcga cacccggcaa tatcgcagcg tccaaaattg
      721 catgaacaaa cccggacagg gctgcgaggg ctgggaggag ttcgagggca ccctgctcgg
      781 cgacgagcag gagacctggc tcaaagaaca gatggcggcc accgccgcga tctggaagtg
      841 catcacccag caggtcgtgt tctcgacggt caacttcggc ggcagcctcg tcaacttcga
      901 ccagtgggac ggctacccca aggcccgcca gcggatcctc gacttcatcg tcgacgagca
      961 gctcgacaac gtcgtcgtgc tctcgggcga catccacctc ggcggcctcg gcgacctgac
     1021 ggcgatcgcc gacgacgacg agtcgcccgt gatcgccacg gagatcgtga cgacctcgat
     1081 cacctcctcc gccaacgtcc cgccggaggc gatcgaaggc gcgctcggcg ggctgccgct
     1141 gctcaagtac gtcaacgccc actcgcgcgg ctacgtcagc cacgacgtca cccgcgcggt
     1201 ctacaccgtc cgctgcgtca tcgtcgactc gatcgtcgac ccgatgtccc cggcgatggt
     1261 cgaggtcgag cgccacatcg acgccggcgt cgccggctat cgccccgtgt gaacgcccct
     1321 ggaccggacc atactttgtg gtatggtccg gcccgatatg ctcaccgtct cgtcgctcgc
     1381 cgcggccgtc gtcgccgcgt gcctgatcgt gctcgcgttc attcacgtat actggggtgc
     1441 cggcggcgcc tggccggcgc gcacccgggc cgggctcggg ccgatggtcg tcggcacacc
     1501 accaggcacc gcgatgccga acctgcccgc ctgcctgctc gtcgccgcgt tgctggtcac
     1561 cgccgctggc ctggtcgcct ccgccggtct cgccgacgaa ccgccgtggt tcgcccggct
     1621 aggcagcgcc ggcgtcgctg gcgtgctcgc gctgcgtggc ctcggcggct tcatcgacgg
     1681 ccgcctgcgc cccgacaccc gcacgcaacc gtactacgcg ctcaatcgca ggatctactc
     1741 cccgctgtgc ctcgcgctcg cggccctgat cgccgtcgtg ctcgcgtgcc gggcatgacc
     1801 cggatgaccc gcgaggactg gctcgacgcc ggtctcgcgc tgctcgccag cgacggccgc
     1861 tgggcgctga cgcaggggcg cctgtgcgcc cgcctcgcgc gcaccaaggg ctcgttttat
     1921 caccacttcg cggacatgct gggctaccag cgcgagctgc tcgcgcgctg gcgcgaacaa
     1981 aacacggagg cgatgatcga gggttcgcgg accgccggcg acccgcgggc ccgcggcgag
     2041 cggctgcgcg aacaggtcgc cgccgccgac ctgcgcgtcg agcgggcgat ccgtgcctgg
     2101 gccgaggtcg agcccgacgc ccgcgcggcg ctcgacgagg tcgacgcccg gcgcctcgca
     2161 tacctgaccg agctggccca tgcccgcgtc gcagatcccg cgcgcgccgt ggtcgtcgcc
     2221 cgcctcgagt acgccgcgtt catcggcacg gtcacgctgt tccacgcgct cccgcaggcc
     2281 cagcgcgagc tcctgagccg cagcctcggc gaggccctcg cgctgtgggc gaacaccacg
     2341 gacgcgtgat cgcgtcagcc gcgtcgcgct ctgccgcggc ccttcgccgc cggcgcctgt
     2401 gcgtcgcgct ggcacagctg cgcgaggtgg gcgagcaccc agcggtgcgc cgggccgagc
     2461 accgtgtcgg gccggtacac cgcgctcagg tgcagcgtgt gctcgtccgg cccccacgcg
     2521 tcgggctgga tccgcagcag cgtgccgccc gcgaggtcgt cggcgacgag cgcctccggc
     2581 aagttgcccc acccgagccc cgcgcgcagc agcgcgtgct tggtgtgcag gtcggcgacc
     2641 cgccaggtgc gcggcgacag cacggcctgg tcgggcacgc cggtcgcgtc gggctcggac
     2701 agaactattt gcacatgccg t
//
LOCUS       DASWNJ010001640         1890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_312784, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001640 DASWNJ010000000
VERSION     DASWNJ010001640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312784"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1538
                     /locus_tag="VGB85_27285"
     CDS             <1..1538
                     /locus_tag="VGB85_27285"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY0137831.1"
                     /translation="LATRNGRVLDLGEPTALTRLQGPRTRVLDLAGGLAVPGLTDAHA
                     HLLGLGAVLEQVDLRGAASVDEVVARVKQHAPESGWVLGRGWDQNLWPGAAMPTHDAL
                     TAAFPDRPVWLRRVDGHAGWANRAALQAARITASTPAPQGGEITLRDGPGGREPTGLL
                     VDAAMELMPVPRADADAFRRRILQAQAQVLPLGLTGVHEMGVSNDADMVLRALATSGE
                     LRLRMTGYADEDWFSRQVRHNMPEKPGHDDRYALVGVKLYVDGALGSRGAALLAPYDD
                     RPGHTGALHHTQAQLGELVEIAVRGGWQVAAHAIGDRAIRDLLDAFEAARKQARGDEG
                     RPRDPARLRVEHAQIVALEDIPRFARLGAVASMQPTHATSDMAWVPARIGAARLPGAY
                     AWRRFLDARVALALGSDFPVEQPNPTHGLHAALTRQDPTGSPPGGWLPDQRLTLWEAI
                     AGFTSGAAFAAGQEDWRGRLGLGMAADLTCFRDDLRTRPPPSVRDAAILATVIAGRVE
                     WEA"
     gene            1597..>1890
                     /locus_tag="VGB85_27290"
     CDS             1597..>1890
                     /locus_tag="VGB85_27290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137832.1"
                     /translation="MMGQRIIAALERRVPEGLRGDLDTRRRSMLALGLCEVMGGTLSP
                     RPRTAAAPSPSACRTPRRPRRPSETHMMDPTSTPDGAVLAAQTTYLDRVAPGHR"
BASE COUNT          248 a          764 c          651 g          227 t
ORIGIN      
        1 cgctggcgac ccgcaacggc cgcgtgctcg acctcggcga gccgaccgcg ctcacgcgcc
       61 tgcaaggccc gcgcacccgc gtgctcgacc tcgccggcgg cctcgccgtg cccggcctga
      121 ccgacgccca cgcgcacctg ctcggcctcg gcgcggtgct cgagcaggtc gacctgcgcg
      181 gcgccgcctc ggtcgacgag gtcgtcgcgc gcgtcaagca acacgcgccg gagtccggct
      241 gggtgctcgg ccgcggctgg gatcagaacc tgtggcccgg cgccgcgatg ccgacccacg
      301 acgcgctgac ggccgccttc cccgaccgcc cggtgtggct gcgtcgcgtc gacggccacg
      361 ccggatgggc caaccgcgcc gcgttgcagg ccgcgcgcat caccgcgagc acgcccgccc
      421 cgcagggcgg cgagatcaca ttgcgcgacg gccccggcgg ccgcgaaccg accggcctgc
      481 tcgtcgacgc cgcgatggaa ttgatgcccg tcccgcgcgc cgacgccgac gcgtttcgcc
      541 ggcggatcct gcaagcccag gcccaggtgc tgccgctcgg gctgaccggc gtgcacgaga
      601 tgggcgtcag caacgacgcc gacatggtgc tgcgcgccct cgcgacctcc ggcgagctgc
      661 gcctgcgcat gaccggctac gccgacgagg actggttctc gcgccaggtc cgtcacaaca
      721 tgcccgaaaa gccaggtcac gacgaccgct acgcgctggt cggcgtcaag ctctacgtcg
      781 acggcgcgct cggcagccgc ggcgccgcgc tgctggcccc ctacgacgac cgccccggtc
      841 acaccggcgc gctgcaccac acgcaggcgc agctcggcga gctggtcgag atcgccgtgc
      901 gcggcggctg gcaggtggcc gcgcacgcga tcggtgaccg tgcgatccgc gacctgttgg
      961 acgccttcga ggccgcgcgc aagcaggccc gcggcgacga aggccgcccg cgcgacccgg
     1021 cgcgcctgcg agtcgaacac gcgcagatcg tcgcgctgga agacatccct cgcttcgcgc
     1081 gcctcggcgc ggtcgccagc atgcagccga cccacgccac cagcgacatg gcctgggtgc
     1141 ccgcacgcat cggcgccgcg cgcctgcccg gcgcctacgc ctggcgccgc ttcctcgacg
     1201 cccgcgtcgc gctcgcgctc ggcagcgact tccccgtcga gcagcccaac ccgacccatg
     1261 gcctccacgc cgcgctgact cggcaagacc ccaccgggtc gcctccgggc ggctggctgc
     1321 cggaccagcg cctgacgctg tgggaagcga tcgccggctt caccagcggc gccgcgttcg
     1381 cggccggcca ggaggactgg cgcgggcgcc tcgggctcgg catggcggcc gacctgacct
     1441 gcttccgcga cgacctgcgc acgcgcccgc cgccgagcgt gcgcgacgct gcgatcctcg
     1501 cgaccgtgat cgccggtcgc gtcgagtggg aggcctgagc aacaccgttc gcgggtggtg
     1561 ttcatcggcc tctcgtggtt gactcgaggc cccggcatga tgggtcagag gatcatcgcg
     1621 gcgctcgagc gccgggtccc cgagggcctg cggggggacc tcgacacccg ccgaaggtcg
     1681 atgctcgcgc tcggactgtg cgaggtcatg ggcggcaccc tctcacctcg accccgaacc
     1741 gcggcagcac cttcaccgtc cgcctgccgt acgccccgac gaccgcgaag accgagtgag
     1801 acacatatga tggacccgac ctcgaccccc gatggcgccg tgctcgcggc gcagaccacc
     1861 tacctcgatc gcgtcgcgcc cggccaccgc
//
LOCUS       DASWNJ010001641         1847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_665446, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001641 DASWNJ010000000
VERSION     DASWNJ010001641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665446"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="VGB85_27295"
     CDS             <1..1062
                     /locus_tag="VGB85_27295"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEY0137833.1"
                     /translation="VPGDSRIVDARPLSRRRGPDTLAPVTADRHATRPPLVTGWSRRG
                     VLRASAVGAASLACGRSAAASASETVLAEARSQFNHVRVAESGTVRTMYFVGDNGMQY
                     IESRVDRAYPASLDLDYTRTMMAGFLLQPRPTRLLMLGLGGGGMSNYLRGRIPGLEID
                     AVDIDPEVVRLAQTYFDVPKDDPRYRCHVEDARLFVERATTQWDMIMLDAFRGVFVPF
                     HLKTQEFYRAVLARLSPRGVVVANLHNVTRMYPHDRETMAAVFPSTYSFVSEGGNQTT
                     LVASADRKRRGPYEVRDNARLAQPTFDFDLMGLAARLYLRRDWDAAEVLHDDFNKDEL
                     KAASERHNETCVKGCKYGL"
     gene            1072..>1847
                     /locus_tag="VGB85_27300"
     CDS             1072..>1847
                     /locus_tag="VGB85_27300"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0137834.1"
                     /translation="MTHPQLRRLKIHRYEQFVDVPWIEFSPHENLILGINGAGKTSLL
                     KLIHAVLTFDYRKLLAQDFDVEFELAYPASSPLQGTVEVSGRVLRTAQVLTDEALRHP
                     ESMDDLNQLEADMEARSGAHRLNFTVLREQATLALDGRDVPVSRKPPRGRVLPWEDAR
                     VGGGPTMLALLPSFSGFFVRESDDDFRALVDDVEFSLAPDSAKRFGIEDARDVPPMLL
                     LALLRHLADLKEEKKIGMRFGRDDLLPDVLTGTRANSFQRL"
BASE COUNT          293 a          668 c          605 g          281 t
ORIGIN      
        1 gtcccggggg acagccggat cgtcgacgcg cgcccgttga gccggcgccg cggaccggat
       61 acgctcgcgc cggtgaccgc agaccgccac gccacccgcc cgccgctcgt cacgggctgg
      121 tcgcgccgcg gggtcctgcg cgcctcggcc gtcggcgcgg cgagcctggc ttgcgggcgc
      181 tcggccgccg cgagcgcgtc ggagacggtg ctcgccgagg cgcgctcgca gttcaaccac
      241 gttcgggtcg ccgagagcgg caccgtgcgc acgatgtact tcgtcggcga caacggcatg
      301 cagtacatcg agtcgcgggt cgaccgggcc tacccggcga gcctcgacct cgactacacg
      361 cgcacgatga tggcgggctt cctgctgcag ccgcggccga cccgcctgtt gatgctcggg
      421 ctcggcggcg gcggcatgag caactacctc cgcggccgga tccccggcct cgagatcgac
      481 gccgtcgaca tcgacccgga ggtcgtgcgc ctggcgcaga cctacttcga cgtccccaag
      541 gacgacccgc gctaccgctg ccacgtcgag gacgcgcgcc tgttcgtcga gcgcgcgacg
      601 acgcagtggg acatgatcat gctcgacgcc ttccgtggcg ttttcgtgcc cttccacctc
      661 aagacccagg agttctaccg ggccgtgctc gcccgcctga gcccgcgagg cgtcgtggtc
      721 gccaacctgc acaacgttac gcgcatgtac ccgcacgacc gcgagacgat ggccgccgtg
      781 ttcccgagca cctacagctt cgtcagcgag ggcggtaacc aaaccaccct cgtcgcctcc
      841 gccgaccgca agcgccgcgg cccctacgag gtccgcgaca acgcccgcct cgcccagccg
      901 accttcgact tcgacctcat gggcctcgcc gccaggttgt acctccgccg cgactgggac
      961 gccgccgagg tcctgcacga cgattttaat aaagacgagc tcaaggccgc cagcgagcgc
     1021 cacaacgaga cctgcgtcaa gggctgcaag tacgggctct gaatggagac catgactcac
     1081 ccccagctgc gacgcctgaa gatccatcgc tacgagcagt tcgtggacgt gccatggatc
     1141 gagttctcgc cgcacgagaa cctgatcctc gggatcaatg gcgccggcaa gacgagcctc
     1201 ctcaagctca tccacgccgt gttgacgttc gactacagga agttgctcgc ccaggacttc
     1261 gacgtcgagt tcgagctggc ctaccctgcg tcctctccgt tgcaggggac ggtcgaggtg
     1321 agcggtcggg tcctccgcac ggcgcaggtg ctgacggacg aggccctccg acaccccgag
     1381 tcgatggacg acctcaacca gctcgaggcc gacatggagg cccgctcggg agcccaccgg
     1441 ctcaacttca cggtgctgcg cgagcaggcc acgctggcac tggacggcag ggacgtgccc
     1501 gtgtcgcgca agcctccgcg aggacgcgtg ctcccctggg aggatgcgcg ggtcggtggt
     1561 ggcccgacga tgctcgcgct cttgccctcg tttagcggct tcttcgtgcg ggagagcgac
     1621 gacgacttcc gcgcgctggt ggacgacgtc gagttttcgc tggccccgga ttcggcgaag
     1681 cgcttcggga tcgaagacgc ccgggacgtc cccccgatgc tcctgctggc gctgctgcga
     1741 cacctcgccg acctgaaaga ggagaagaag atcgggatga ggttcggccg tgacgatctg
     1801 ctgcccgatg tgctcaccgg aacgcgcgcg aacagcttcc agcgcct
//
LOCUS       DASWNJ010001642         3417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_411775, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001642 DASWNJ010000000
VERSION     DASWNJ010001642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411775"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VGB85_27305"
     CDS             <1..324
                     /locus_tag="VGB85_27305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137835.1"
                     /translation="GPQAPTPAVRVVERASDSMQAPAPVGGPVLDPGYSGLGQLSVRL
                     FFSKMLAATPEGLTIDFTRWQSASVERFFLAITSPGLIRRREAPTTGDERLTLPTAFD
                     GFEWD"
     gene            340..825
                     /locus_tag="VGB85_27310"
     CDS             340..825
                     /locus_tag="VGB85_27310"
                     /inference="COORDINATES: protein motif:HMM:NF012712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137836.1"
                     /translation="MNTEIQTMLSASEGRYPTRAEQAVLREWASKLDARLEAIDVIRS
                     AEENIIGQTLDEVLVTYPNVEKHYKNVRSSGTRDMSLVLRYCAQAMLRGDIKYLDDSI
                     LTWMSTILRGVGLAPQFIEDTYRALARVAARELPPQVAELLRPYIERCAQVLPGRPET
                     P"
     gene            822..1454
                     /locus_tag="VGB85_27315"
     CDS             822..1454
                     /locus_tag="VGB85_27315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137837.1"
                     /translation="MTERRDLNYFTSPNFLRFDVKSGVLRSRGGTRMIAVNEDFLRGF
                     VAACEHETGPAASLVLRRCGEFFGRRLARRFEAELGQFASVSLRDRSMAEFDALVRDL
                     WDGCGLGRIEIDWDRGQHGFLPIRLADSPMQDIGPSGHTGDDMFAGILCGFFSGFCDA
                     DMRSVQTGDLRLGDKEGTTFIVAIVEIVRRVEGMRANKQRHSEIVAALAG"
     gene            1476..2990
                     /locus_tag="VGB85_27320"
     CDS             1476..2990
                     /locus_tag="VGB85_27320"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0137838.1"
                     /translation="MEQELRAAPTTVLGRYVLGERLAAGSLGTVVAATDPRTGRAVAV
                     KFFDGASDNYAAWVDEMRLAVRLAHPHIVACIDAGHDDAWGLSVLVFARAMGGSLRRA
                     LASGKVFDGPAVHRLLTEISAALALAHEQGVVHRDIKPENILALERLGEPPWALTDFG
                     AGRFLPRGAALRSLAGSTGYIAPEVTLGAADATSDLYSLGVVALELYTGERGELATRT
                     QFRLRHRTGDGPAAVIARLLAPDPNDRFPGAAALVAALAAAPTGLDVGHTQDGAAWLL
                     AGDEVSRRCPETGRLVPLGRVPRARRFIHEPDDDAVIIAGDRRVVCLDPARPGSRPAT
                     LLASDEGFAAFVASRRHAAIWLLQAGQLVRSDLAGERVHWRVPLPPEWLAALHPHPPV
                     HALTGLVLAHDQALLGLLGCPLLLRVDRVPQGLRLTPLRAPAPLYALVRRGPELLIHC
                     GDAGSAALLRLDGDGLQLLQLLHHRALPVDCVGVTDTPQGPRLTDLVQVPEAHV"
     gene            2983..>3417
                     /locus_tag="VGB85_27325"
     CDS             2983..>3417
                     /locus_tag="VGB85_27325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137839.1"
                     /translation="MSELEWIERTLLAAGLVSPGRLAAWTQGWQRETSLLRHLVARGV
                     LDSAAARTIGAVIQGYVQMAPDTVVRLFRGGEADVPADMPQKPGPATREPSRREPAPR
                     HAPPAGRVRTRQSDGPGDMSLGTSAAPASASAPTLQETSPARA"
BASE COUNT          417 a         1346 c         1191 g          463 t
ORIGIN      
        1 gggccgcagg ccccgacgcc ggcggtgcgc gtcgtcgagc gcgcgtcgga ctcgatgcag
       61 gcgccggcgc ccgtcggcgg tccggtgctc gacccgggct acagcggcct cgggcagctg
      121 agcgtgcggc tgttcttcag caagatgctg gcggcgacgc ccgagggcct gacgatcgac
      181 ttcacgcgct ggcagtcggc ctcggtcgag cgcttcttcc tggcgatcac ctcgccgggc
      241 ctgatccgcc gccgcgaggc gccgacgacc ggcgacgagc gcctgacctt gccgaccgcc
      301 ttcgatggct tcgagtggga ctgacccgag gagagcacga tgaacaccga gattcagacg
      361 atgttgagcg cgagcgaggg gcgctacccc acgcgcgccg agcaggcggt cctgcgcgag
      421 tgggcgagca agctggacgc ccggctcgag gccatagacg tgatccgcag cgccgaggag
      481 aacatcatcg gccagaccct cgacgaggtc ctggtgacct acccgaacgt cgagaagcac
      541 tacaagaacg tgcgcagcag cggcacccgc gacatgtcgc tggtgctgcg ctactgcgcg
      601 caggcgatgc tgcgcggcga catcaagtac ctcgacgaca gcatcctgac ctggatgtcg
      661 acgatcctgc gcggcgtcgg gctcgcgccg cagttcatcg aggacaccta ccgcgcgctc
      721 gcccgggtcg ccgcgcgcga gctgccgccg caggtcgccg agctgctgcg cccctacatc
      781 gagcgctgcg cccaggtcct gcccggccgc ccggagaccc catgaccgag cgccgcgacc
      841 tcaactactt cacgagcccg aacttcctcc gcttcgacgt caagtccggc gtgctgcgca
      901 gccgcggcgg cacccgcatg atcgccgtca acgaggactt cctgcgcggc ttcgtcgccg
      961 cctgcgagca cgagaccggc ccggccgcgt cgctggtgtt gcgccgctgc ggcgagttct
     1021 tcgggcgccg tttggcccgc cgcttcgagg ccgagctcgg ccagttcgcg tcggtgtcgc
     1081 tgcgggaccg cagcatggcg gagttcgacg cgctggtccg cgacctgtgg gacggctgcg
     1141 ggctcggccg catcgagatc gactgggacc gcggccagca cggcttcctc ccgatccgcc
     1201 tcgccgacag cccgatgcag gacatcggcc cgagcggcca caccggcgac gacatgttcg
     1261 cgggcatcct gtgcggcttc ttctccggct tttgcgacgc cgacatgcgc agcgtgcaaa
     1321 ccggcgacct gcgcctcggc gacaaggagg gcaccacctt catcgtcgcg atcgtcgaga
     1381 tcgtgcgccg cgtcgagggc atgcgggcca acaaacaacg tcacagcgag atcgtcgccg
     1441 cgctcgccgg ctagtcgatt attggaggag ccccggtgga acaggagctg cgcgccgcgc
     1501 cgacgaccgt gctcggtcgc tatgtcctcg gcgagcggct cgccgcgggg tcgctcggca
     1561 ccgtcgtcgc ggcgaccgac ccccgcaccg ggcgcgccgt cgccgtcaag ttcttcgacg
     1621 gcgcctccga caactacgcc gcgtgggtcg acgagatgcg cctcgccgtc cgcctcgccc
     1681 acccgcacat cgtcgcctgc atcgacgccg gccacgacga cgcctggggc ctgtcggtgc
     1741 tggtgttcgc gcgggcgatg ggcggctcgc tgcggcgcgc gctcgccagc ggcaaggtct
     1801 tcgacggccc ggccgtacac cgcctgctga ccgagatctc ggccgcgctc gcgctggcac
     1861 acgagcaggg cgtcgtgcat cgcgacatca agcccgagaa catcctcgcg ctcgagcgcc
     1921 tcggcgagcc gccgtgggcg ctgaccgact tcggcgccgg ccgcttcctg ccccgcggcg
     1981 ccgcgctgcg ctcgctcgcg gggtcgaccg gctacatcgc cccggaggtg accctcgggg
     2041 ccgccgacgc gacctcggac ctgtactcgc tcggcgtcgt cgcgctcgag ctgtacaccg
     2101 gtgagcgcgg cgagctggcg acgcgcacgc agtttcgcct gcgccaccgc actggcgacg
     2161 gcccggccgc cgtgatcgcc cgcctgctcg cgcccgaccc gaacgaccgc ttccccggcg
     2221 ccgcggcgct ggtcgctgcc ctcgcggccg cgccgaccgg cctcgacgtc ggccacaccc
     2281 aggacggcgc cgcctggctg ctcgccggcg acgaggtctc gcgccgctgc cccgagaccg
     2341 gccggctggt accgctcggt cgggtcccgc gcgcgcgtcg cttcatccac gagcccgacg
     2401 acgacgccgt gatcatcgcc ggcgaccgtc gcgtggtctg cctcgacccc gcgcgcccgg
     2461 gctcgcgacc ggcgacgctg ctcgccagcg acgagggctt cgcggccttc gtcgcctcgc
     2521 gccggcacgc cgcgatctgg ctgttgcagg ccgggcagct ggtccgctcc gacctcgccg
     2581 gggagcgcgt gcactggcgc gtgccgctgc cgcccgagtg gctcgccgcg ctccaccccc
     2641 atccgcccgt gcacgccctc acgggcctcg tgctcgcgca cgaccaggcg ctgctcgggc
     2701 tgctcggctg cccgctgctg ctgcgggtcg accgcgtgcc gcagggcctg cgcctgaccc
     2761 ccctgcgcgc cccggccccg ctgtacgcgc tggtgcgccg cgggcccgag ctgctgatcc
     2821 actgcggcga cgccggctcg gcggcgctgc tgcgcctcga cggcgacggc ctgcagctgc
     2881 tgcagttgct gcatcatcgc gcgctgccgg tcgactgcgt cggcgtcacc gacacgccgc
     2941 agggcccgcg cctgaccgac ctcgtgcagg tcccggaggc ccatgtctga gctcgagtgg
     3001 atcgagcgca cgctgctcgc cgccggcctg gtctcgcccg gccggctcgc ggcctggacg
     3061 cagggctggc agcgcgagac ctcgctgctg cgccacctcg tcgcccgcgg cgtgctcgac
     3121 tcggccgccg cccgcacgat cggcgccgtc atccaggggt acgtgcagat ggccccggac
     3181 acggtcgtgc gcctgttccg cggcggcgag gcggatgtcc ccgcggacat gccgcaaaag
     3241 ccaggtcccg cgacgcgcga gccgagccgc cgcgagccgg ccccgcgaca cgccccgccg
     3301 gccggccgcg tcaggacgcg acagtcggat ggccccgggg acatgtccct cgggacatcc
     3361 gccgcgcccg cgtccgcgtc ggcgccgacg ctccaggaga cctccccggc ccgcgcg
//
LOCUS       DASWNJ010001643         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_451109, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001643 DASWNJ010000000
VERSION     DASWNJ010001643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451109"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2083
                     /gene="carB"
                     /locus_tag="VGB85_27330"
     CDS             <1..>2083
                     /gene="carB"
                     /locus_tag="VGB85_27330"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY0137840.1"
                     /translation="CSIENVDPMGVHTGDSITVAPALTLTDKEYQIMRNASIAVLREI
                     GVETGGSNVQFAVNPRDGRLVVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLD
                     EIDNDITGVTPASFEPSIDYVVTKIPRFAFEKFRDSEALLSTAMKSVGEVMAIGRSFH
                     ESLQKALRGLETGLSGLDDVVALQGAPAAEIEAALSRPTPDRLLVAAQALREGFSVAE
                     VAAITKYDPWFLERIAEIVEAEQRVSRDGLPTDAQGLRALKSMGFSDKRLADLAVRSL
                     HLRSDARALSRGTGLIAETVRALAGATTEEEVRRLRRRLGVRPVFKRIDTCAAEFDAK
                     TPYMYSTYEAPSFGNPEDESQPTGRRKIAILGGGPNRIGQGIEFDYCCVHACFALRDA
                     GFETIMINCNPETVSTDYDTSDRLYFEPLTAEDVLEVLETERSNGELVGVIVQYGGQT
                     PLKLAHAIHAAGIPILGTQPDAIDLAEDRERFAGLIDRLGLRQPLNGIARSREEALLV
                     AERIGYPVLTRPSYVLGGRAMEIVDGPAQLEDYIQTAVQVSGDSPVLIDQYLRDAIEV
                     DVDALGDGTDVVVAGVMQHIEEAGVHSGDSACSLPPYSLEPDVIAEIERQTRVLAEAL
                     GVIGLMNIQFAVKGGLVYLIEVNPRASRTVPFVAKAIGRPVAKIAARVMAGEPLSNFD
                     PIGRELGHIAIK"
BASE COUNT          346 a          622 c          796 g          319 t
ORIGIN      
        1 ctgctcgatc gagaatgtcg atccgatggg ggtgcatacc ggggattcga tcacggtcgc
       61 gccggcgctg acgctgaccg acaaggaata tcagatcatg cgcaacgcca gcatcgcggt
      121 gctgcgcgag atcggggtcg agaccggcgg gtccaacgtc cagttcgcgg tcaatccgcg
      181 cgacgggcgg ctggtggtga tcgagatgaa cccgcgggtg tcgcgttcct cggcgctggc
      241 gtcgaaggcg acgggtttcc cgatcgcaaa ggtcgcggcg aagctggcgg tgggctacac
      301 gctcgacgag atcgacaacg acataaccgg cgtgacgccg gcgagcttcg agccgtcgat
      361 cgactatgtc gtgaccaaaa ttccgcggtt cgccttcgag aagttccgcg attccgaggc
      421 gctgctttcc accgcgatga agtcggtcgg cgaggtgatg gcgatcgggc gctcgttcca
      481 cgagagcctg cagaaggcgc tgcgggggct ggagacgggc ctgagcgggc tcgacgacgt
      541 tgtggcgctg cagggcgcgc cggctgcgga gatcgaggcg gccttgtcgc ggccgacgcc
      601 ggaccggctg ctcgtcgcgg cgcaggctct gcgcgagggc ttctcggtgg ccgaggtggc
      661 ggcgatcacc aagtacgacc cctggttcct ggagcggatc gccgagatcg tcgaggccga
      721 gcaaagggtg agccgcgacg gcctgccgac cgacgcgcag gggctgcggg cgctcaagag
      781 catgggcttt tcggacaaaa ggctcgcgga cctggcggtg cgcagcctgc acctcaggag
      841 cgacgcgcgg gcgctttcgc gggggaccgg gctgatcgcc gagacggtgc gggcgctggc
      901 cggggcgact acggaggagg aagtgcggcg gctgcggcgg cggctcggtg tgcggccggt
      961 gttcaagcgg atcgacacct gcgcggccga gttcgacgcc aagacgccgt acatgtactc
     1021 gacctacgag gcgcccagct tcggtaaccc ggaggacgag agccagccga cggggcggcg
     1081 caagatcgcc atcctgggcg gcgggccgaa ccggatcggg caggggatcg agttcgatta
     1141 ttgctgcgtc cacgcctgct tcgcgctgcg cgacgccggc ttcgagacga tcatgatcaa
     1201 ctgcaatccg gaaacggtga gcaccgatta cgacacgtcc gaccggctct atttcgagcc
     1261 gctgaccgcc gaggacgtgc tcgaggtgct ggagacggag cgctcgaacg gcgaattggt
     1321 cggggtcatc gtccaatatg gcgggcagac gccgctgaag ctggcgcacg cgatccacgc
     1381 ggcggggatc ccgatcctgg gaacgcagcc cgacgcgatc gacctggccg aggatcgcga
     1441 gcggttcgcg gggctgatcg acaggctggg cctgaggcag ccgctcaacg gcatcgcgcg
     1501 cagccgggaa gaggcgctgc tggtggcgga gcggatcggc tacccggtac tgacgcggcc
     1561 ctcctacgtg ctgggcgggc gagcgatgga gatcgtcgac gggccggcgc agctggagga
     1621 ttatatccag accgcggtgc aggtgagcgg cgacagcccg gtgctgatcg accagtatct
     1681 gcgcgacgcg atcgaggtgg acgtcgatgc gctcggcgat ggcaccgatg tcgtggtggc
     1741 gggcgtgatg cagcacatcg aggaggcggg agtccattcg ggcgacagcg cctgctcgct
     1801 gccgccctat agcctggagc cggacgtgat tgccgagatc gagcggcaga cacgggtgct
     1861 ggccgaggct ttgggcgtga tcgggctgat gaacatccag ttcgcggtga agggagggct
     1921 ggtctacctc atcgaggtca atccgcgggc gagccgcacc gtgccgttcg tcgccaaggc
     1981 gatcgggcgg cccgtcgcga agattgccgc ccgggtgatg gccggcgagc cgttgagcaa
     2041 tttcgatccg atcggccgcg agcttgggca tatcgcgatc aag
//
LOCUS       DASWNJ010001644         2309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_312895, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001644 DASWNJ010000000
VERSION     DASWNJ010001644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312895"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VGB85_27335"
     CDS             complement(<1..361)
                     /locus_tag="VGB85_27335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137841.1"
                     /translation="MPRIFVAQTLADSWLAAGRVQLERDLLRISDVGGGGITDLFINP
                     AVFFERIDGSEADVHDVVGRVKTAQELAQMGADHYESSVVMGDYAYTVVPGYLAIPVD
                     GKGTELRVDATSWGRIVN"
     gene            501..839
                     /gene="trxA"
                     /locus_tag="VGB85_27340"
     CDS             501..839
                     /gene="trxA"
                     /locus_tag="VGB85_27340"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY0137842.1"
                     /translation="MASDKVKISSDSEFQADVLDTSKDQLVVVDFWATWCGPCRSMAP
                     HLDAIAGEYDGKIRIVKVDVDRNQKSAITYGIQNLPTLLVFKGGEVVDKLVGNPGSKG
                     KLEEWMKRSL"
     gene            1522..2163
                     /locus_tag="VGB85_27345"
     CDS             1522..2163
                     /locus_tag="VGB85_27345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137843.1"
                     /translation="MSTSTTGEGGSTGTQAASTSTTVTPTSADTTEAIDATTTSSGTE
                     TTSDTDFDTTTDEMPWGECAEPYVDWDKEHVDCMVDWPTMTAVHGEGPATGLVSVTRI
                     YFAVDYYACTPDGLTLGWLVIDDPTTPTAVLYGYASCGPERWIGEHDRKGEILGSGTP
                     IGVTMTIDGFAGDWISADPIDPPRILGSFTGDLVGPFEAIHCAALDTAIWNCG"
BASE COUNT          385 a          819 c          768 g          337 t
ORIGIN      
        1 gattgacgat ccgcccccac gacgtcgcgt cgacccgcag ctcggtgccc ttgccgtcga
       61 ccgggatcgc cagatacccg ggcaccaccg tgtacgcgta gtcgcccatc accaccgacg
      121 actcgtagtg atcggccccc atctgcgcca gctcctgcgc cgtcttcacg cgcccgacga
      181 cgtcgtgcac gtcggcctcc gagccgtcga tgcgctcgaa gaagaccgcc gggttgatga
      241 agaggtccgt gatcccgccg ccgccgacat cggagatgcg gaggagatcg cgctcgagct
      301 gcacgcggcc agcagcgagc cacgagtcgg cgagggtctg ggcgacgaag atccgtggca
      361 tgacccgtcc agggtagtcc cgcaggccct cggtagacaa caccttgcgc gacccctccg
      421 cccgacccat catgccagcc tgccgcgagg ggggccatcc cggcccccgg ccacaaaccc
      481 gcatcccgga acacacgacc atggcatccg acaaagtaaa aatttcctcc gactccgaat
      541 ttcaggcgga tgtcctcgac acctccaagg accagctcgt ggtcgtcgac ttctgggcca
      601 cctggtgcgg cccctgccgc tcgatggcgc cccacctcga cgcgatcgcc ggcgagtacg
      661 acggcaagat ccgcatcgtg aaggtcgacg tcgaccgcaa ccaaaagagc gcgatcacct
      721 acgggatcca gaacctcccg accctgctcg tgttcaaggg cggcgaagtc gtcgacaagc
      781 tggtcggcaa ccccggcagc aagggcaagc tcgaagagtg gatgaagcgc agcctctgac
      841 gctcccctct cgctcgtttg cgtgacacac agggcccccg ccggggccct tttttgtgtc
      901 ggtgaacggc ggcgcggccg cctcgctacg gcgtgaggac gcggcgcctg ctcggtcggg
      961 tgcgggtgtc agatcgctat gcgggcgtgc ctggacgtgg cgcggacctc ccggggccgg
     1021 gcgtgttcgg gacattgcgg ggcgaggccg catcgagctg gcgtgttcgg ggtaacgcgg
     1081 ggcgaggctg tttgggccgg ggagggccga cggactgctg gtccctgcga cgcgggtcct
     1141 cccaggggac atgctcgccg ccccatctgt cccgaaagac agctggtttc accgaccctg
     1201 cgtcgccaca ccgcgcgttg acggggcgtc tgcgcgtgac ccctgggtcc tcccgctgct
     1261 tcccccgctc gtcggccccc aaccccgggc ggacaccgtc tgcgccccgc ttgtcgaaga
     1321 tgcactccgt cgcgcgaccg cgggccaaca tctcgcgtct cgcgtatcac ccgatcgtcg
     1381 gcgctggcgg tcgcggacgg atctatgcat gctcgtacga tgcgacgata cctgcgagtg
     1441 ttcgcgctga cctccattgc gggcgctgca tgtggccccg agagtccggc cggcacgagt
     1501 gccgggacct cgggcgctga tgtgtccacc agcacgacag gcgaaggtgg cagcacgggc
     1561 acgcaggcgg cgagcacgag cacgacggtg acccccacgt cggccgacac caccgaggcc
     1621 atcgacgcga caacgacgtc gtccggcaca gaaacgacct ccgacacgga cttcgacacg
     1681 acgaccgatg aaatgccgtg gggggagtgc gctgaacctt acgtggactg ggacaaggag
     1741 cacgtcgact gcatggtcga ctggccgacg atgacggccg tacacggcga gggcccggcg
     1801 accgggctcg tgtcggtgac gcgcatatac ttcgcggtgg attattacgc ctgcaccccc
     1861 gatgggctga cgctcgggtg gctcgtcatc gatgatccga ccacgccgac ggcggtcctg
     1921 tacggatacg cctcgtgcgg ccccgagcga tggatcggcg aacacgaccg caagggtgag
     1981 atccttggat ccggcacacc gatcggcgtc accatgacga tcgacggctt cgccggcgac
     2041 tggatcagcg cggacccgat cgacccgcca cggatcctcg gcagcttcac gggcgacctg
     2101 gtcggcccat tcgaggccat ccactgcgcc gcgctcgaca ccgcgatctg gaactgcggc
     2161 tgactcccgg cgggtccgcg cacgcaagct tccggcgctc ctcacgacgc ccgcgtcacc
     2221 gaagcggggc gcagacgatg tccgcccggg gttgggggcc gacgagcggg ggaagcagcg
     2281 ggaggaccca ggggtcacgc gcagacgcc
//
LOCUS       DASWNJ010001645         3741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_29245, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001645 DASWNJ010000000
VERSION     DASWNJ010001645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29245"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /locus_tag="VGB85_27350"
     CDS             complement(<1..1373)
                     /locus_tag="VGB85_27350"
                     /inference="COORDINATES: protein motif:HMM:NF016099.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019290 - siderophore biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IucA/IucC family protein"
                     /protein_id="HEY0137844.1"
                     /translation="MTTQNIESIRVAGARRVAQAIGELCFEGLLTATPAGDGIYTMIL
                     GPATYRFRGRASAWGGLHVEVGSVTRQIDGAEEPVDSPVGLLLDARAQLGASDEVLAE
                     WLAEIHGSLLAEAEQCEHMRELDAAALANLSGVALEQHLDGHPKLVAHRGRVGWGFED
                     LRAHAPECGARVRIYWLAVAPELAHCSGAAPLVSESCDAPEQARLSQQLHTSRLPDAV
                     LVPVHPWQWQRHVAPQYTRQLARGEIVPLGVFGDLYAPRMSVRTLANVDRPERADLKL
                     ALGILNTSCWRGLPGEHVAQGPGVAAALRRRVEADPLLCAANLKVLCDLGGVHVPHPE
                     FAALPGAPYRLRELLAAVWRESAATRLGPDEVEVPAAGLQQCDLAGAPLIREWVDRSG
                     VSLARWLQALFEHTAVPLYHLLCAHGLGAIAHGQNLGLVLREGLPVGALLRDVHGDLR
                     RLDDDTL"
     gene            complement(1370..>3741)
                     /locus_tag="VGB85_27355"
     CDS             complement(1370..>3741)
                     /locus_tag="VGB85_27355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016099.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0137845.1"
                     /translation="LAEPALARALGVDDAATAERLVARVRTSEANLDATLAARAADIE
                     ALLHAPLGFIAAEQGLLAGHSVHPAPRLREGLAAGDAHCVPELGGRVTLHLWAVARER
                     LLQGGSDAQARLDALLASDSAWAQAVAGDRVVLPLHPLQDRALREHPSLAAARRRGEI
                     EPLGPCGRAWAVTSSLRTLHADGAPYMLKHSLPVRLTNSRRTLSLVELERGLLLGRVL
                     AQPGLAELAQRHPNFHLLCEPAYFGLRDDDGGPGPADSFVALRDNPLRATQPAEMLAT
                     LLQDHPHTGRSRLALRIEAAARAAGERPLDRATRWFADYLRVVLAPVVSAQADHGLLL
                     SAHQQNLVIGLAADGLTPAAAWFRDAQGTAYTELALRRHGEQVPGLAQALFTAPLGER
                     VWAYSTVINAVFNVIAALAMIPGVAQSLLLAQLRACLLDLRAADPADPRALDYLVRSP
                     ELWTKANVRCFAGGVDEVALRDPLAIYTRMKNPLWQAQVTDTAIPGGRVAGIAGEAVV
                     DMHEGRIDLGDETHPFTWQAPVLTTSAASDLLVDHLFTRSPTLTHVRVGAQTIVRAAF
                     YQRPRPWHHRGAAPPPPGAPTSTGTIMHPSRPRDSASLLYRRHCAAIDRTFSLHRFDP
                     DRDLDAFCRWMNDPVVAQFWEQDWSRERLSTYIEQRLADPHVIPAIGRFDDHAFAYFE
                     IYWAKEDRLGPHYAADDFDRGFHMAVGDPQVRHRGWGRQWFLAMAHFLFLDDARTMRL
                     VGEPRLDQARVRGWSASTPWQEWGEVQFPHKRAALMVLTRERFFEDFRE"
BASE COUNT          523 a         1342 c         1408 g          468 t
ORIGIN      
        1 tcgagggtgt cgtcgtcgag gcggcgcaga tcgccgtgga cgtcgcgcag cagggcgccg
       61 accggcaggc cctcgcgcag cacgaggccg aggttctgtc cgtgggcgat cgcgccgagg
      121 ccgtgcgcgc acagcaggtg atacagcggg accgcggtat gctcgaacaa cgcctgcaac
      181 catcgtgcga gcgagacgcc gctgcgatcg acccactccc ggatcagcgg cgcaccggcg
      241 aggtcgcact gctgcaaccc ggccgcgggc acctcgacct cgtcgggtcc gagccgcgtc
      301 gccgcgctct cgcgccagac cgcggcgagc agctcgcgca ggcgatacgg cgcgccgggc
      361 agcgcggcaa attccgggtg tggcacgtgc acgccgccga ggtcgcacag caccttcaaa
      421 tttgcggcgc acagcagcgg gtcggcctcg acgcggcgac gcagcgcagc ggcgacgcca
      481 gggccctgcg cgacgtgctc gccgggcagg ccgcgccagc acgaggtatt gaggatgccg
      541 agcgcgagct tgaggtcggc gcgctccggt cggtcgacgt tggcgagcgt gcgcaccgac
      601 atccgcgggg catacagatc tccgaacacg ccgagcggga cgatctcgcc gcgcgcgagc
      661 tgccgcgtgt actgcggcgc gacgtggcgc tgccactgcc acggatgcac gggcacgagc
      721 accgcgtcgg gcaggcggga tgtgtgtagt tgctgagaca gccgcgcctg ctcgggggcg
      781 tcgcagctct cggagaccag cggcgcggcg ccgctgcaat gcgcgagctc cggggcgacc
      841 gccagccagt atatgcgtac ccgggcgccg cactcgggcg cgtgggcgcg caggtcctcg
      901 aacccccagc cgacgcggcc gcggtgggcg acgagcttgg ggtgaccgtc gaggtgctgc
      961 tcgagcgcga cgcccgagag gttcgcgagc gccgccgcgt cgagctcccg catatgctcg
     1021 cactgctcgg cctcggcgag caggctgccg tggatctcgg cgagccactc cgcgaggacc
     1081 tcgtcgctgg cgccgagctg cgcccgcgcg tcgagcagca ggccgaccgg cgaatcgacc
     1141 ggctcctcgg cgccgtcgat ctgccgggtg acgctgccga cctcgacgtg caaaccgccc
     1201 cacgcgctcg cgcggccacg aaagcggtat gtggccggcc cgaggatcat cgtataaatg
     1261 ccgtcgcccg cgggcgtggc ggtcagcagg ccctcgaagc acagctcgcc gatcgcctgc
     1321 gcgacccgcc gtgccccggc gacacggatg ctctcgatgt tttgcgtggt cattccctga
     1381 agtcctcgaa gaagcgctcg cgcgtcagca ccatcagcgc cgcgcgcttg tgcgggaact
     1441 ggacctcgcc ccactcctgc cacggcgtgc tcgccgacca gccgcgcacg cgcgcctggt
     1501 cgagcctcgg ctcgccgacg aggcgcatgg tccgggcgtc gtcgaggaac aggaagtggg
     1561 ccatcgccag gaaccattgc cggccccagc cgcggtggcg cacctgcgga tcgccgacgg
     1621 ccatgtggaa cccgcgatcg aagtcgtcgg cggcgtagtg cggcccgagc cggtcctcct
     1681 tggcccagta gatctcgaag tacgcgaagg cgtgatcgtc gaagcgaccg atcgccggga
     1741 tcacgtgcgg gtcggcgagg cgctgctcga tgtacgtcga gagtcgctcg cgcgaccagt
     1801 cctgctccca gaactgcgcg accaccgggt cgttcatcca gcgacaaaac gcgtcgaggt
     1861 cgcggtcggg atcgaagcgg tgcaggctga aggtgcggtc gatcgcggcg cagtgcctgc
     1921 gatacagcag cgacgcgctg tcgcgcggac gcgagggatg catgatcgtg ccggtggacg
     1981 tcggggcgcc cgggggcggc ggcgcggcgc cgcggtgatg ccacggacgt ggtcgctggt
     2041 aaaatgccgc gcgcacgatc gtctgcgcgc cgacgcggac gtgcgtgagc gtcgggctgc
     2101 gcgtgaacag gtgatcgacg agcaggtcgc tcgccgcgct cgtggtgagc acgggcgcct
     2161 gccaggtgaa cgggtgggtc tcgtcgccga ggtcgatgcg gccctcatgc atgtcgacca
     2221 ccgcctcgcc cgcgatcccg gcgacgcgtc cgccggggat cgccgtgtcg gtcacctgcg
     2281 cttgccacag cgggttcttc atccgcgtgt agatcgccag cgggtcgcgc agggcgacct
     2341 cgtcgacgcc gccggcgaag cagcgcacgt tggccttggt ccacagctcc ggcgagcgca
     2401 cgagatagtc gagcgcgcgc gggtcggcgg ggtcggccgc gcgcaggtcg agcagacagg
     2461 cgcgcagctg ggcgagcagc agcgactgcg cgacgccggg gatcatcgcc agcgcggcga
     2521 tcacgttgaa cactgcgttg atcaccgtcg agtaggccca cacgcgctcg ccgagcggcg
     2581 cggtgaacag cgcctgcgcg agccccggga cctgctcgcc gtggcggcgc agcgcgagct
     2641 cggtataggc ggtgccctgc gcgtcgcgga accacgccgc ggccggcgtc aggccgtcgg
     2701 cggcgaggcc gatcacgagg ttctgctggt gggcgctcag cagcaggccg tggtcggcct
     2761 gggcgctgac caccggcgcg agcacgaccc ggaggtagtc cgcgaaccat cgcgtcgcgc
     2821 ggtcgagcgg ccgctcgccg gccgcgcgcg cggccgcttc gatccgcagg gcgagccgac
     2881 tgcgacccgt gtgcgggtga tcttgcagca gggtcgccag catctcggcc ggctgcgtcg
     2941 cgcgcagcgg gttgtcgcgc agcgccacga agctgtccgc cgggccaggt ccaccgtcgt
     3001 cgtcgcgcag gccgaagtac gccggctcgc acagcaggtg aaaattcggg tggcgctgcg
     3061 cgagctcggc gaggcccggc tgcgcgagca cccgcccgag cagcaggcca cgctccagct
     3121 ccaccagcga cagcgtgcgc cgcgagttgg tcaggcgcac cggcagcgag tgcttgagca
     3181 tgtacggcgc gccgtcggcg tgcagcgtgc gcagcgacga ggtcaccgcc cacgcgcgcc
     3241 cgcacggccc gagcggctcg atctcgccgc gccgccgcgc cgccgcgagc gacggatgct
     3301 cgcgcagcgc ccggtcctgc agcggatgca gcggcaggac cacgcgatcg ccggcgaccg
     3361 cctgcgccca cgcggaatca ctggcgagca gcgcatcgag gcgcgcctgc gcgtccgatc
     3421 ctccttgcag cagtcgttcg cgcgcgaccg cccacaggtg cagcgtcacg cgaccgccga
     3481 gctcgggcac gcagtgcgcg tcgccggcgg cgaggccctc gcgcagccgc ggcgccgggt
     3541 ggaccgagtg cccggcgagc aagccctgct cggccgcgat gaagccgagc ggcgcgtgca
     3601 gcagcgcctc gatgtcggcc gcgcgagccg cgagggtcgc gtcgaggttg gcctcgctgg
     3661 tgcgcacgcg ggcgaccagc cgctccgcgg tcgcggcgtc gtcgaccccg agcgcgcgcg
     3721 ccagggccgg ctcggcgagc a
//
LOCUS       DASWNJ010001646         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_665554, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001646 DASWNJ010000000
VERSION     DASWNJ010001646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665554"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1279
                     /locus_tag="VGB85_27360"
     CDS             <1..1279
                     /locus_tag="VGB85_27360"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY0137846.1"
                     /translation="LPAMILRDPVHGLISFTGAAEGWISRLLACPEVQRLRRIRALGL
                     ASLAFPGGEHSRFAHALGSAHVMQRYLDRVRTLEAELPPHDRIDDTTALAALAAALLH
                     DLGHGPYSHTFEAVLPDSPRHETWTSQILLDPDTAVHRVLAEVDASMPAVVERLIHGQ
                     SPVGHLARAVSGTFDVDRCDYLMRDSYMTGVRYGLLDVDWLLASLRLYVPKDSTSARL
                     VVDGEKGLTAVEGFFLARLYMYRQVYLHKAVRAAERMIGALFTRLLELGPQPGTPPVL
                     ADILLGRMVSVGAYLELDDTSLITAIAAYARGNDPVLAELASGLHDRRLFKTLRLAPG
                     ADSKEAQARLDAVLVAAGVGPAYLGVIDCVQIDAYTDDEAIGVLRSGGRVQQLVDASP
                     VLRGLSRERFVHHRAIFPPSVRARVVEALADLT"
     gene            1292..1960
                     /locus_tag="VGB85_27365"
     CDS             1292..1960
                     /locus_tag="VGB85_27365"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF015875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HEY0137847.1"
                     /translation="MPRKSTRPRCPVCGLHLELCLCDALPLRDLSTGFVVVQHNRERN
                     KPTSTGRVTAAVLRAPVIFYGAQGESLDTTALTDPDTDYFVLFPTEDAPPVDDAATRP
                     APGRRRCFVILDGTWQQCSRMARRAPEISKMQRLALPPGPPSRWGVRTPPNAAAVCTF
                     EAATRLVALLHGDEAAAPMDSFFTELSSRMLGMRGRVAGDEPDDLETAEETAEETAEE
                     TAEA"
BASE COUNT          270 a          806 c          657 g          300 t
ORIGIN      
        1 gctccccgcg atgatcctgc gcgatcccgt tcacgggctg atctcgttca ccggcgcggc
       61 cgaggggtgg atctcgcggc tgctcgcgtg tccggaggtc cagcgcctgc gccggatccg
      121 cgctctcggg ctcgcgtcgc tcgccttccc cggcggtgag cactcgcgct tcgcccacgc
      181 cctcggctcg gcgcacgtga tgcagcgcta cctcgaccgc gtgcgcacgc tggaggccga
      241 gctgccgccg cacgaccgca tcgacgacac cacggcgctc gccgcgttgg ccgccgcgct
      301 gttgcacgac ctcgggcacg gcccttactc gcacaccttc gaggcggtgc tgcccgactc
      361 gccgcggcac gagacctgga cctcgcagat cctgctcgac cccgacaccg ccgtgcatcg
      421 cgtgctcgcc gaggtcgacg cgagcatgcc cgccgtcgtc gagcgcctga tccacgggca
      481 gagcccggtc gggcacctcg cgcgcgccgt ctccggcacc ttcgacgtcg accgctgcga
      541 ctacctcatg cgcgacagct acatgaccgg cgtgcgctac ggcctgctcg acgtcgactg
      601 gctgctcgcc tcgctgcgcc tgtatgtccc gaaggacagc acctccgcgc gcctcgtcgt
      661 cgacggcgag aagggcctga ccgccgtcga gggcttcttc ctcgccaggc tctacatgta
      721 ccgccaggtc tatttacaca aggccgtgcg cgccgccgag cgcatgatcg gcgcgctgtt
      781 cacccgcctg ctcgagctcg gcccgcagcc cggcacacca cctgtcctcg cggacatctt
      841 gctcgggcgc atggtctcgg tcggcgccta cctcgagctc gacgacacct cgctgatcac
      901 ggcgatcgcc gcctacgcgc gcggcaacga ccccgtgctc gccgagctcg cgagcggtct
      961 gcacgaccgg cgtttattta aaacgctgcg cctcgcgccg ggggccgact cgaaggaggc
     1021 ccaggcgcgc ctcgacgccg tgctcgtcgc ggcgggcgtc ggcccggcct acctcggcgt
     1081 gatcgactgc gtgcagatcg acgcctacac cgatgacgag gcgatcggcg tgctgcgctc
     1141 cggcggtcgc gtgcagcagc tggtggacgc ctcgccggtg ctgcgcggcc tcagccgcga
     1201 gcgcttcgtc catcaccggg cgatcttccc gccgagcgtg cgcgcccgcg tcgtcgaggc
     1261 cctcgccgac ctgacctgag agacagatcc catgccccgc aagtccaccc gcccccgctg
     1321 tcccgtctgc ggcctgcacc tcgagctgtg cctgtgcgac gccctgccgc tgcgtgacct
     1381 ctcgaccggc ttcgtcgtcg tccagcacaa ccgcgagcgc aacaagccga cgagcaccgg
     1441 tcgggtcacc gccgccgtgc tgcgcgctcc tgtgatcttt tacggggcgc agggcgagtc
     1501 gctcgacacc accgcgctga ccgaccccga caccgactac ttcgtgctgt tcccgaccga
     1561 ggacgccccg cccgtcgacg acgcggcgac ccggccggcc ccggggcgcc ggcgctgctt
     1621 cgtcatcctc gacggcacct ggcagcagtg ctcgcgcatg gcccggcgcg cccccgagat
     1681 ctcgaagatg cagcgcctcg ccctcccgcc cggtccgccg tcccgctggg gcgtgcgcac
     1741 gcccccaaac gccgccgccg tgtgcacctt cgaggccgcc acccgcctcg tcgcgctgct
     1801 ccacggggac gaggccgccg cgccgatgga cagctttttt acggagctca gcagccgcat
     1861 gctcggcatg cgcgggcgag tcgccggcga tgagccggac gacctcgaga ccgcggagga
     1921 aaccgcggag gaaaccgcgg aggaaaccgc ggaggcttaa aaagccgaaa gcctcgaggg
     1981 cggaccctcg aagctttctt gtgcggggcg gacgggactc gaacccgcga cct
//
LOCUS       DASWNJ010001647         2523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_313001, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001647 DASWNJ010000000
VERSION     DASWNJ010001647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313001"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..1744
                     /locus_tag="VGB85_27370"
     CDS             86..1744
                     /locus_tag="VGB85_27370"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEY0137848.1"
                     /translation="MSFVRALHVPVLASLTLAPFADAAADEGTLTTIDQPEQAPRVCD
                     LGAPPEVNDHPPPVNCAESKDTGYMSGNPFEITVVHIDGKPVEKSTANAYWVMREAAA
                     AAGVDMHINSGFRTMSEQQYFYNCYVNCNCNNCNQAAKPGYSNHQSGHALDLNASAPG
                     VYNWLAAHGGAYGFKETVASENWHWEWWGGGPKGGICDISAPPKGYLDAADCDKVNGW
                     AQDPDAPDAAIQVHVYFNGPAGDAAAIGVPVTANEHREDLCQALGSCAHGFTLGIPLS
                     LRDNAPHPVHAYGIDTEGGDNTQLVQSPGSFTCAPPALPPGVRRHVSNPEILAAWSFD
                     TFWQMVKLDDATLLAIPEWTAIDPAPLLIRADDGTPEVWLVDGPVRRHVPNPQVAANW
                     EFDLGTVVTTPAADVYAIPVGTPVWDAPILVKGAGPAVYQLDDRQCYPDSPDPECQEP
                     DATTGGPTSDTANDTSSATASDPSGDPTPTSGPEETTAASVGDAGGDEPSGTGSTSGD
                     TTGDTTGAANDDADGCGCRTDAPASPRLALGLLVLGLLARRRRA"
     gene            complement(1842..>2523)
                     /locus_tag="VGB85_27375"
     CDS             complement(1842..>2523)
                     /locus_tag="VGB85_27375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137849.1"
                     /translation="IVLVSACGGKSSTDTDTDTGSETTVAQTDPSGSSSGASTSGETP
                     TSGATESDDTGTGEATTGAPDEGMACAGYCEYQVNCWMEDLAVCLDTCEAELAGSAYI
                     GPDCVAEQLEYYQCYGELTCADGEEGEKACAGQGDGPAPPSTACILPACETQCAKDAE
                     CGGDPAVEECAQSCSYFIGLFVAEGGEDCGKAYEALGTCTGSLTCEQYANQTGCEAEI
                     AAIETSCE"
BASE COUNT          390 a          951 c          819 g          363 t
ORIGIN      
        1 cgagcacaca ccatccgacc acggggtcgc gctcgcggtt cgtgcgcacg cgcgggccgt
       61 tcgggctccg tgataccgtg ccgccatgag cttcgtgcgc gcgcttcacg tccctgtcct
      121 cgcgtcgctg accctggctc cgttcgccga cgcggcggcc gacgagggca ctttgaccac
      181 gatcgatcag cccgagcagg cgccgagggt ctgtgacctc ggcgccccgc cggaggtgaa
      241 cgaccacccg ccgccggtca actgcgccga gtccaaggac accggctaca tgagcggcaa
      301 cccgttcgag atcaccgtcg tgcacatcga cggcaagccg gtcgaaaaga gcaccgcgaa
      361 cgcctactgg gtcatgcgcg aggccgcggc cgccgctggc gtcgacatgc acatcaacag
      421 cggctttcgc acgatgtccg agcagcagta cttttataat tgctacgtca attgtaactg
      481 taacaattgc aatcaggccg ccaagcccgg ctactcgaat caccagagcg gccacgcgct
      541 cgacctcaac gcatccgccc ccggtgtgta caattggctc gccgcgcacg gcggggccta
      601 cgggttcaag gagacggtgg cgagcgagaa ttggcactgg gagtggtggg gcggcggccc
      661 gaagggcggg atctgtgaca tcagcgcgcc gcccaagggt tacctcgacg cggcggactg
      721 cgacaaggtc aacggctggg cgcaggaccc cgacgcgccc gacgctgcga tccaggtgca
      781 cgtatatttc aatggtccgg caggggacgc cgctgccatc ggtgtgcccg tgaccgccaa
      841 cgagcaccgc gaagatctgt gccaggcgct cgggtcgtgc gcccacggct tcacgctcgg
      901 catcccgctc agcctgcgcg acaacgcccc gcaccccgtg cacgcctacg gcatcgacac
      961 cgagggcggt gacaacacgc agctggtcca gagccccggg tcgttcacct gcgccccgcc
     1021 ggccctgccg ccgggcgtgc gccgccacgt ctccaacccg gagatcctcg ccgcctggag
     1081 cttcgacacc ttctggcaga tggtgaagct cgacgacgcg acgctgctgg cgatccccga
     1141 gtggacggcg atcgatccgg cgccgctgtt gatccgcgcc gacgacggca cccccgaggt
     1201 gtggctcgtc gacggcccgg tgcgtcgcca cgtgccgaac ccgcaggtcg ccgccaactg
     1261 ggagtttgac ctcgggaccg tcgtcaccac cccggccgcg gatgtctatg cgatcccggt
     1321 cggcacgccg gtgtgggacg caccgatctt ggtgaagggg gccgggcccg ccgtgtatca
     1381 gctcgacgac cggcaatgtt atcccgacag ccccgacccc gaatgtcagg agcccgacgc
     1441 gaccaccggc ggaccgacga gcgacaccgc gaatgacacc tcgagcgcga ccgcgagtga
     1501 cccctccggc gacccgacgc cgacgagcgg ccccgaggag acgaccgcgg cctccgtcgg
     1561 cgatgccggc ggcgacgagc cgagcggcac cggatcgacg tcgggcgaca cgaccggcga
     1621 cacgaccggc gccgccaacg acgacgcgga tggttgcggc tgccggaccg acgccccggc
     1681 gtcgccccgc ctcgcgctcg gcctgctggt cctgggcctg ctcgcccgcc gccgccgggc
     1741 ctgaggtctg cgtcatcggc ccgcgcatgg cgacagcgcc acgcgcggtg ccgcgatccg
     1801 gatgacgtca cgaagcgccg cgccgcacgc ccgggtcgcg ctcactcgca gctcgtctcg
     1861 atcgcggcta tctcggcctc gcagcccgtc tggttggcat actgctcgca ggtcaggctg
     1921 cccgtgcagg tcccgagcgc ctcgtatgcc ttgccgcaat cttcgccgcc ctcggccacg
     1981 aacaggccga tgaaatagct acagctctgc gcgcactcct cgaccgccgg gtcgccaccg
     2041 cactccgcat ccttggcgca ttgcgtctcg caagcgggca ggatgcaggc cgtgctcggc
     2101 ggtgccggcc cgtcgccttg ccccgcgcag gccttctcgc cctcctcgcc gtccgcacag
     2161 gtcagctcgc cgtagcactg gtagtactcg agctgctcgg cgacgcagtc cggcccgatg
     2221 tacgcggacc cggcgagctc ggcctcacag gtatccaggc agacggcgag gtcctccatc
     2281 cagcagttca cctggtactc gcaatagccc gcgcaggcca tcccctcgtc cggggcgccg
     2341 gtcgtcgcct cgcccgtgcc ggtatcatcc gactcggtcg cgcccgaggt cggcgtctcg
     2401 ccgctcgtgc tcgcaccgga gctggagccg gacgggtcgg tctgagcgac cgtcgtctcc
     2461 gaaccagtat ccgtatccgt gtccgtcgac gacttgccac cgcaagccga cacgagcacg
     2521 atc
//
LOCUS       DASWNJ010001648         2026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_313090, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001648 DASWNJ010000000
VERSION     DASWNJ010001648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313090"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(267..1733)
                     /locus_tag="VGB85_27380"
     CDS             complement(267..1733)
                     /locus_tag="VGB85_27380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEY0137850.1"
                     /translation="MSVLPPLQGPEVFTARRRRLAARLGSVPAAVFAGDLVPKNYAAN
                     IYPYRAHSHFLYLTGIHWPGAVLLGEGDEWEIFAAPPAPDDALWIGPSASWETLAAAC
                     GVQAIRPLSDLRDAVIASGGAEAIATLPAIHPRTRAQQASLLGRPWGLHDESQHGAIA
                     LEAHDSTLADAMIALRLVQDDAALELLRSASEAARAGHLAGMAATAPGKRESDVRAAI
                     EREFTARGCSPAYGSIVSVSGEILHNEHSHQVMRAGDLLLVDAGAEFHGWASDVTRTW
                     PVSGQFSPSQRALYDIVLQANIDAIEKVRPGARYRDVHLQACRTLTRGLVDEGLLRGE
                     VDGLVERGAHALFFPHGVGHLMGLDVHDMEDLGDRAGYAPGRKRSGQFGLGYLRLDRD
                     LAPGMVVTIEPGLYFVPAILDDPELCGGFDRDGTLNRERLAAFADVRGIRIEDDVRCT
                     AGAPEVLTVAIPKTADAVAGLVGADGAGRSGQRTEDAA"
     gene            1811..>2026
                     /locus_tag="VGB85_27385"
     CDS             1811..>2026
                     /locus_tag="VGB85_27385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137851.1"
                     /translation="MTTSAVDRDPPPTRLARGVMPSRRLLLWLTLLALASRLLWVLLV
                     HPPGDYVFSDMGMYVKRATDLAVHGPRS"
BASE COUNT          268 a          718 c          761 g          279 t
ORIGIN      
        1 caatggaacg agcggagcgg gctgccggcg gtggtatgcc tggtccagtt ttgcggctgg
       61 tccagttttg cggccgcaag gggccaggtc gcggggccag ggtaccatga gcgtctatcc
      121 gttgctacgc gccagcgcaa ggaacgggcg cgggtttcag ggtgactctc atcgacgcct
      181 gggcgggccg cccggagggt ccggcggggc gtgtgcgggg gacgggtgcg gaggtcgggc
      241 gggccgcgcg atccgggcgc gagcgctcag gcggcgtcct cggtgcgctg cccggaccgg
      301 ccggcgccgt cggccccgac gagaccggcg accgcgtccg cggtcttggg gatcgccacc
      361 gtcaggacct cgggggcgcc ggcggtgcag cgcacgtcgt cctcgatgcg gataccccgg
      421 acatccgcga aggcggcgag gcgctcgcgg ttgagggtgc cgtcgcggtc gaagccgccg
      481 cacagctccg ggtcgtcgag gatcgccggc acgaaataga ggcccggctc gatcgtgacg
      541 accatgcccg gcgcgaggtc gcggtcgagg cggagataac cgagcccgaa ttgcccgctg
      601 cgcttgcgcc cgggggcata ccccgcccga tctccgagat cctccatgtc gtgcacgtcg
      661 aggcccatca ggtgaccgac gccgtgcggg aagaacagcg cgtgggcccc tcgctcgacc
      721 agaccgtcga cctcgccgcg cagcaggccc tcgtcgacca ggccgcgggt cagcgtgcga
      781 caagcttgca gatgcacgtc gcggtagcgc gcgcccggcc ggaccttctc gatcgcgtcg
      841 atgttggcct gtaggacaat gtcgtacagg gcgcgctggc tcgggctgaa ctgcccggag
      901 accggccagg tgcgggttac gtcgctggcc cagccgtgga actcggcgcc ggcgtcgacg
      961 agcaggagat ctccggcgcg catgacctgg tggctgtgct cgttgtgcag gatctccccg
     1021 gagacggaga cgatgctgcc gtaggcgggc gagcagccgc gggccgtgaa ctcgcgctcg
     1081 atcgcggccc ggacgtcgct ctcgcgcttg ccgggggccg tcgccgccat gccggcgagg
     1141 tgacccgcgc gcgcggcctc ggaggcgctg cgcagcagct cgagcgcggc gtcgtcctgg
     1201 accaggcgca gggcgatcat cgcgtcggcg agggtgctgt cgtgggcctc gagggcgatc
     1261 gcgccgtgct gggactcgtc atgtaaaccc caggggcgcc cgagcagcga agcctgttgc
     1321 gcgcgggtgc gggggtggat cgccggcagg gtggcgatcg cctcggcgcc gccgctggcg
     1381 atcacggcgt cgcgcaggtc cgacagcggg cggatcgcct ggaccccgca ggcggccgcg
     1441 agggtctccc agctcgcgct gggaccgatc cacagggcgt cgtccggggc cggcggggcg
     1501 gcgaagatct cccactcgtc gccctcgccc agcaacaccg cgcccggcca gtgaatgccg
     1561 gtcaggtaga gaaagtggct gtgggcccgg tacgggtaaa tgttggccgc gtagttcttc
     1621 ggcaccagat cgcccgcgaa cacggccgca ggcaccgaac ccagccgggc cgccaggcgg
     1681 cggcgacgcg cggtgaacac ctctggaccc tggagcggcg gcagcacgga catgacctcc
     1741 cttttaccgc gcccccgtcc caccaggaac ccctcccgca cccgccggcc gtcgtctacc
     1801 ctcccgcacc gtgacgacct ccgctgtcga ccgcgacccg cccccgacgc gcctcgctcg
     1861 cggcgtcatg cccagccgcc gcctgctcct ctggctgacg ctgctcgcgc tggccagccg
     1921 gctgctgtgg gtgctgctcg tgcacccgcc cggcgactac gtgtttagcg acatggggat
     1981 gtacgtgaag cgcgcgaccg acctcgcggt ccacggtccg cgcagc
//
LOCUS       DASWNJ010001649         3016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_594973, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001649 DASWNJ010000000
VERSION     DASWNJ010001649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594973"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VGB85_27390"
     CDS             <1..765
                     /locus_tag="VGB85_27390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HEY0137852.1"
                     /translation="WSIDVGTGQRVAATVVQVRRATRECLALRWQGGSLVCTPDHPLY
                     SPETGTYRPASDWVTGPARTLLLSLEDGVRVVAVEGSETFAGMHEVIDLSLDLEPHNF
                     VAAGVVVHNKSPATEIAEDEQAGPTVELGPGESASFEIHVCADGEDHPELGFGVDVVG
                     KTGEVPKDNVTFGLSVDFDDPEEGLRGTIPFDDSLTSRSEAPCEDGVILTLSRTDAGT
                     GGSIVVSWTASARVVFVPAEGDEVVDSVEITIEALP"
     gene            complement(831..2258)
                     /gene="boxB"
                     /locus_tag="VGB85_27395"
     CDS             complement(831..2258)
                     /gene="boxB"
                     /locus_tag="VGB85_27395"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HEY0137853.1"
                     /translation="MVSTTVDLNSKIPNNVGLSDDVKLQRALEKWLPSYLEWWNDMGP
                     SDFADKPVFLRTAVSVDSDGWAHYEHVKMPDYRWGIFLNPRAPETSLVRCGDDVGKPA
                     WNEVPGEHRNALRRIIVTQADTEPASVEQQRLLGHCAPSLYDMRNLFQVNVEEGRHLW
                     AMVYLLHKYFGRDGRDEADELLQRRSGDPDKPRILGAFNQPCDHWLSFFAFTMFTDRD
                     GKYQLAALSQSGFDPLSRTTEFMLTEEAHHLFVGETGMDRIIKRSAELQKQDPDGDIR
                     RAGGIDFDLIQRTINYWFTYSLDLFGGEISSNAADFFATGLKGRYREDRYDEHKALAG
                     TYRLPVVEDGRLTERDVPLRNAMNEVLRDSYVLDCERALRKWNRTLEEAGSPTRLSLP
                     SRRFHRHQGIYADHRFDPQGNLIDANRFEANQKNWLLTGADNDYLLSIMKPVYEPGKF
                     ANWIAPPNKGIEGRPMDFEYVRLND"
     gene            complement(2286..>3016)
                     /locus_tag="VGB85_27400"
     CDS             complement(2286..>3016)
                     /locus_tag="VGB85_27400"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970554.1"
                     /note="cleaves the ring of
                     2,3-dihydro-2,3-dihydroxybenzoyl-CoA forming
                     6-hydroxy-3-hexenoyl-CoA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="benzoyl-CoA-dihydrodiol lyase"
                     /protein_id="HEY0137854.1"
                     /translation="LDDALLQIRFNHLDIGLVVLRTAGDPAHVLAHDAALWAAREHWF
                     TNEVLRHMGRTLRRLDLTSRSFYALGDKHAGSKPAFAGCLLELALAADRFYLLDDPDV
                     QVGPSVLNEGALPMSHGLPRLAARLYGEPDKLEALVGKHALMSAEDADEAGLVTVRLD
                     DIDWDDDTRVAIEERSSMSPDALTGMEANLRFPGAENENSKIFARLTAWQNWIFIRPN
                     ATGPEGALSLYGKPQRPHFDWNRV"
BASE COUNT          429 a          924 c         1165 g          498 t
ORIGIN      
        1 tggtcgatcg acgtcggcac ggggcaacgc gtcgcggcga cggtcgtgca ggtgcgtcgc
       61 gcgacgcggg agtgcctggc gctgcgctgg cagggcgggt cgctggtgtg cacgcccgac
      121 cacccgctct actcgcccga gaccggcacc taccgcccgg cctcggactg ggtgaccggg
      181 ccggcgcgca cgctgctgct ctcgctcgag gacggcgtgc gcgtcgtcgc cgtcgagggc
      241 agcgagacct ttgccggcat gcacgaggtc atcgacctct cgctcgatct cgagccgcac
      301 aacttcgtcg cggccggcgt ggtggtgcac aacaagagcc cggcgacgga gatcgccgag
      361 gacgagcagg cggggccgac cgtggagctc ggacccggag agagcgcgag cttcgagatc
      421 cacgtctgcg ccgacggcga ggaccacccc gagctcggct tcggggtgga cgtggtcggc
      481 aagacgggcg aggtgccgaa ggacaacgtc accttcgggc tcagcgtcga cttcgacgac
      541 ccggaggagg gcctgcgtgg gacgatcccc ttcgacgaca gcctcaccag ccgttccgag
      601 gcgccgtgcg aggatggcgt catcctgacg ctgagccgca ccgacgcggg tacgggcggg
      661 tcgatcgtgg tgagctggac ggcgagcgcg agggtcgtgt tcgtgccggc cgagggcgac
      721 gaggtggtcg actcggtcga gatcacgatc gaagcgctgc cctgaccgcg aaacgagcga
      781 cgcccgcggc tccagcggag cgcgggcgtc ggggtctagc gagagacggg ctagtcgttg
      841 aggcgcacgt actcgaagtc catcgggcgg ccctcgatgc ccttgttggg cggggcgatc
      901 cagttggcga acttcccggg ctcgtagacc ggcttcatga tcgacagcag gtagtcgttg
      961 tcggcgccgg tcagcagcca gttcttctgg ttggcctcga agcggttggc gtcgatcagg
     1021 ttgccctgcg ggtcgaagcg gtggtcggcg tagatgccct ggtgacggtg gaagcggcgg
     1081 ctcggcagcg agagccgcgt cggcgagccg gcctcctcga gggtgcggtt ccacttgcgg
     1141 agcgcgcgct cgcagtcgag cacgtagctg tcgcgcagga cctcgttcat cgcgttgcgc
     1201 agcgggacgt cgcgctcggt caggcggccg tcctcgacga cgggcaggcg gtaggtgccc
     1261 gcgagcgcct tgtgctcgtc gtagcggtcc tcgcggtagc ggcccttgag gccggtggcg
     1321 aagaagtcgg cggcgttgct cgagatctcg ccgccgaaca ggtcgaggct gtaggtgaac
     1381 cagtagttga tcgtgcgctg gatgaggtcg aagtcgatgc cgccggcgcg acggatgtcg
     1441 ccgtcggggt cctgcttctg cagctcggcc gagcgcttga tgatgcggtc catgccggtc
     1501 tcgccgacga agaggtggtg ggcctcctcg gtgagcatga actcggtcgt gcgcgacagc
     1561 gggtcgaagc cgctctgcga cagcgcggcg agctggtact tgccgtcgcg gtcggtgaac
     1621 atcgtgaacg cgaagaagct cagccagtga tcgcagggct gattgaacgc gccgaggatc
     1681 cgcggcttgt ccgggtcgcc gctgcgacgc tgcagcagct cgtcagcctc gtcgcggccg
     1741 tcacgaccga agtacttgtg cagcaggtag accatcgccc acaggtgccg gccctcctcg
     1801 acgttgacct ggaacaggtt gcgcatgtcg tagaggctcg gcgcgcagtg accgagcagg
     1861 cgctgttgtt caacgctggc gggctcggtg tcggcctggg tgacgatgat gcggcgcagg
     1921 gcgttgcggt gctcgccggg gacctcgttc cacgcgggct tgccgacgtc gtcgccgcag
     1981 cggaccagcg aggtctcggg ggcgcgcggg ttgaggaaga tgccccagcg gtagtccggc
     2041 atcttgacgt gctcgtagtg ggcccagccg tcgctgtcga cggacacggc ggtgcgcagg
     2101 aacaccggct tgtcggcgaa gtcggacggg cccatgtcgt tccaccactc gaggtagctg
     2161 gggagccact tctcgagggc gcgctggagc ttgacgtcgt ccgacagtcc gacgttgttg
     2221 gggatcttgg agttgaggtc gacggtggtg ctgaccatga ttgcgagtgc tcctgtcgga
     2281 cggatctaga cgcggttcca atcgaagtgc gggcgctgcg gcttgccgta gaggctgagc
     2341 gcgccctcgg ggccggtggc gttgggacgg atgaagatcc agttctgcca cgcggtgagg
     2401 cgcgcgaaga tcttgctgtt ttcgttctcg gcgccgggga agcgcaggtt ggcctccatg
     2461 ccggtgagcg cgtcgggtga catgctcgag cgctcctcga tcgccacgcg ggtgtcgtcg
     2521 tcccagtcga tgtcgtcgag acgcacggtc acgaggccgg cttcatcggc gtcctcggcg
     2581 ctcatcagcg cgtgcttgcc gaccagcgcc tcgagcttgt cgggctcgcc gtacaggcgg
     2641 gccgcgaggc gcggcaggcc gtggctcatc ggcagcgcgc cctcgttgag cacgctgggg
     2701 ccgacctgca cgtcggggtc gtcgagcaga tagaagcggt cggccgcgag ggccagctcg
     2761 agcaggcaac cggcgaaggc gggcttgctg ccggcgtgct tgtctcccaa ggcgtagaac
     2821 gagcggctgg tgaggtcgag gcgacgcagg gtgcggccca tgtggcgcag gacctcgttg
     2881 gtgaaccagt gctcgcgggc ggcccacagc gcggcgtcgt gggcgagcac gtgggccggg
     2941 tcgcccgcgg tgcgcaggac cacgaggccg atgtcgaggt ggttgaagcg gatctgcagg
     3001 agggcgtcgt cgagct
//
LOCUS       DASWNJ010001650         3222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_625374, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001650 DASWNJ010000000
VERSION     DASWNJ010001650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625374"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VGB85_27405"
     CDS             <1..915
                     /locus_tag="VGB85_27405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137855.1"
                     /translation="MTPRTARIALAVFATVAALEWIAAARAYRATLTPAVWQRTGETL
                     AALPPDEPVYLGTAWLGPSARQHLPPLRTPASLAPPDLYGVPRFHVLGLAGSPHAGGW
                     SDDLQTDLGELPPPTALGVDELGPLRLHHYAQASTSAPLTDWLAAPSALLLGDDRGPC
                     RLSRETWTCKQGRVAVETLEVAFRARHCLAVALDDGATLEVTHPHAQLGAQLRGHLGF
                     SDFNARLRNDSPALLELFIDETLAARWTISDSQGWAAFAAPTTPGAHAVRLRLTPLLG
                     GTWTEQGYAATPRRTACLELRALAEAAP"
     gene            912..2699
                     /locus_tag="VGB85_27410"
     CDS             912..2699
                     /locus_tag="VGB85_27410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137856.1"
                     /translation="MSPRALRLTAAALGLATVVLLALGHREVGYVRDEGIYFAASRQH
                     AAWVAQVFTSPREALAAPARDRAFTVNREHPALLKIAAGVSARLFARPGPPGPEHAPA
                     GLLPILPEGAAMRLPAQLLAALGVALLFLAGAGLAAPVRRDSSTRDGAPDSPATAPPG
                     APASPVPSDSHPTPPAPSDSLPLLAGLCAALWFIALPHVWFHAGLHAFDVPVAVATLA
                     VVLIYRRALHSPRWGLALGPALGVAISIKHNALFIPVLLALHYGICLLLALRRPSPAQ
                     LVPLPFLAMAVLAPLTVVALWPWLWSDPPGRVLEYLEFHRFHAYYNTEYFNINYNRPP
                     LPVSYPFVLTWATVPTGMLLLALTGLARGLARAFATSPGPKDSPRWTAPLAGQDRRDG
                     LLWALFAAFPLLLIALPTIPIFGGTKHWLTAYPFFALGSASALIWLVRAAALPPRWRH
                     APAITLLLLLVPGVWSTIDGHPYGLSQYAPFIGGPRGAADLGLLRGFWGHAVPASVLD
                     PTAPGAPIYLHDMHELARQQYAREGRWPPGATSVPPARARAALIFHERHMLTYELQVQ
                     DALETASPVEVVTLDDVPLTSLYRADQPR"
     gene            2712..>3222
                     /locus_tag="VGB85_27415"
     CDS             2712..>3222
                     /locus_tag="VGB85_27415"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY0137857.1"
                     /translation="MLDHEARVQAVAAQIRERARAGQPVHIDKGGVHHVVPIPGDPRF
                     RSGKLDTSALRQILEIDRDHMRCVAEPGVSFRDLVRATLPLGLVPTVVPELEGITLGG
                     AIAGGAVESMSYRHGGFFDSVESLEIITGTGEVLELSPTQHPELFHMIHGSYGTLGVI
                     SRVSFRLIPA"
BASE COUNT          374 a         1379 c          984 g          485 t
ORIGIN      
        1 atgacccccc gcaccgcacg gatcgcactc gccgtgttcg ccacggtcgc cgcgctcgag
       61 tggatcgcag cggcgcgggc ctatcgcgcg acgctcaccc ccgccgtgtg gcagcggacc
      121 ggcgagaccc tcgctgcgct gccgcccgac gagccggtgt atctcggcac cgcatggctc
      181 ggaccgagcg cgcgccagca cctgcctccg ctgcgcacgc ccgcctcgct cgcgccgccg
      241 gacctgtacg gcgtgccgcg ctttcacgtg ctcggcctgg ccggctcgcc gcacgccggt
      301 ggctggtccg acgacctgca gaccgacctc ggcgagctcc cgcccccgac cgcgctcggc
      361 gtcgacgagc tcggcccgct gcgcctgcat cactacgcgc aggcatcgac gagcgccccg
      421 ctcaccgact ggctcgccgc cccgtccgcg ctgctcctcg gcgacgaccg cggcccctgt
      481 cgcctgtccc gagagacatg gacctgcaag cagggccgcg tcgccgtgga gaccctcgag
      541 gtcgccttcc gcgcgcgtca ctgcctcgcc gtggcgctcg acgacggcgc gaccctcgaa
      601 gtcacccacc cccacgcgca gctcggcgcg cagctgcgcg gccacctcgg cttctccgac
      661 ttcaacgcgc gcctgcgcaa cgactccccc gcgctgctcg agctgttcat cgacgagacc
      721 ctcgccgcgc gctggacgat ctccgacagt cagggctggg ctgcatttgc cgcgccgacc
      781 accccgggcg cccacgccgt gcgcctgcgc ctgaccccgc tgctcggcgg cacctggacc
      841 gagcagggct acgccgccac cccgcggcgc accgcgtgcc tcgagctgcg cgcgctcgcg
      901 gaggcagccc cgtgagcccc cgcgccctgc gcctcaccgc cgccgccctc ggcctggcga
      961 cggtcgtgct gctcgcgctc ggccaccgcg aggtcggcta cgtgcgcgac gagggcatct
     1021 acttcgccgc gagtcgccag cacgccgcct gggtcgcgca ggtgttcacg tccccgcgcg
     1081 aggccctagc ggccccggcc cgcgaccgcg ccttcaccgt caaccgcgag caccccgcgc
     1141 tgctgaagat cgcagccggc gtgtccgccc gcctgttcgc gcgacccggc ccgcccggcc
     1201 ccgagcacgc ccccgccggc ctgctcccga tcctccccga gggcgccgcg atgcgcctgc
     1261 ccgcccagct gctcgccgcc ctcggcgtcg ccctcctgtt cctcgctggc gccggcctgg
     1321 ccgcacctgt ccgaagggac agctcgaccc gcgacggagc tccggacagc cccgcgaccg
     1381 cccccccggg agctccggcc tcgcctgtcc cttcggacag ccacccgacc ccacctgccc
     1441 cttcggacag cctccccctg ctcgccggcc tgtgcgccgc gctgtggttc atcgccctgc
     1501 cgcacgtatg gttccacgcc ggcctgcacg ccttcgacgt cccggtcgcg gtcgccaccc
     1561 tcgcggtcgt cctgatctac cgccgggccc tgcacagccc gcgctggggc ctcgcgctcg
     1621 gcccggcgct cggcgtggcg atctcgataa agcacaacgc gttgttcatc cccgtgctgc
     1681 tagcgctgca ttatgggatc tgcctgctgc tcgccctccg gcgcccatca cctgcccagc
     1741 tcgtcccgct cccattcctg gccatggccg tgctcgcgcc gctgacggtc gtcgcgctgt
     1801 ggccgtggct gtggtccgac ccgcccggcc gggtgctcga gtacctcgag tttcaccgct
     1861 tccacgccta ctacaacacc gagtatttta atatcaacta caaccgcccg ccgctgccgg
     1921 tcagttatcc ctttgtgctg acctgggcca ccgtgccgac cggcatgctg ctgctcgcac
     1981 tgaccggcct cgcccgcggc ctcgcccgcg cgttcgccac gtcacctggc ccaaaggaca
     2041 gccctcgctg gaccgccccg ctcgccggcc aggaccgccg cgacggcctg ctgtgggccc
     2101 tgttcgccgc cttcccgctg ctgctgatcg ccctcccgac gatcccgatc ttcggcggca
     2161 ccaagcactg gctgaccgcc tacccgttct tcgccctcgg ctccgcctcc gccctgatct
     2221 ggctggtgcg cgcggcagct ttgccgccgc gctggcgcca cgccccggcg atcacgctgc
     2281 tgttgctcct cgtaccgggc gtgtggagca cgatcgacgg ccacccgtac ggcctctcgc
     2341 agtacgcgcc cttcatcggt ggtccgcgcg gcgccgctga cctcggtcta ctgcgtggct
     2401 tttggggtca cgccgtcccg gcctccgtgc tcgaccccac cgcccccggc gcgccgatct
     2461 acctccacga catgcacgag ctggcgcgcc agcaatacgc ccgcgagggc cgctggccgc
     2521 ccggcgccac ctccgtcccg cccgcgaggg cccgcgccgc gctgatcttc cacgagcgcc
     2581 acatgctgac ctacgagcta caggtccagg acgccctcga aacagcgtct ccggtcgagg
     2641 tcgtcaccct cgacgacgtc ccgctgacga gcctgtaccg cgccgaccag ccgcggtgat
     2701 aaaaccgggg catgctcgac cacgaggccc gcgtccaggc ggtcgccgcc cagatccgcg
     2761 aacgtgcgcg cgccggtcag ccggtgcaca tcgacaaggg cggcgtgcat cacgtcgtgc
     2821 cgatccccgg cgacccgcgg tttcgcagcg gcaagctcga cacctccgcg ctgcgccaga
     2881 tcctcgagat cgaccgggac catatgcgct gcgtcgccga gcccggcgtc tcgtttcgtg
     2941 acctcgtgcg cgcgaccctg ccgctcggcc tcgtgccgac cgtcgtgccc gagctcgagg
     3001 gcatcaccct cggcggcgcg atcgcgggcg gcgcggtcga gtcgatgtcc taccgtcacg
     3061 gcggcttctt cgacagcgtc gagtcgctcg aaattatcac cggcaccggc gaggtcctcg
     3121 agctgtcgcc gacgcagcac cccgagctgt tccacatgat ccatgggtca tacgggaccc
     3181 tcggcgtcat cagccgcgtc agctttcgcc tgatccccgc ca
//
LOCUS       DASWNJ010001651         3098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_277131, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001651 DASWNJ010000000
VERSION     DASWNJ010001651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277131"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VGB85_27420"
     CDS             complement(<1..614)
                     /locus_tag="VGB85_27420"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEY0137858.1"
                     /translation="MSVADGKAVVTPSMGGLATGLKGPFERSQGLWIGWPGDLSGLGE
                     EEQAGALRELEKLRTVPVQLTAEEVRSYYEDFSNGVLWPLFHYLLDRVPLDSTGWDSY
                     MRVNARFADAVAEKYEPGDVIWVQDYQLLLLPQLLRERLPEAKIGFFLHIPFPASEVF
                     RMLPWRAEVLRGMLGADLVGFHTPSYARNFASAVMRILGHEPTF"
     gene            941..2089
                     /locus_tag="VGB85_27425"
     CDS             941..2089
                     /locus_tag="VGB85_27425"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY0137859.1"
                     /translation="MSSREIWLASLLLTACGGSPSDTTGTTGTEATGATSTGVETTGT
                     GEPTTTTTMTTPDTTTDTPTGPSTTDTTTDTTGGSTTGGTTGSTTGSTTDTTTDPVDP
                     PASLEDVVDGELEMIADGHQFTEGPVWSLEGHLLYSDIPANTIFKWVEGGASEPFITP
                     SGNSNGLAFDSMGRLLAAEHGNRRISRRVLGEDAQPVADGFEGKQLNSPNDLIIRSDG
                     TIYFTDPPYGIQPNQQEQPFNGVFRVDTQGQLSLVSDDFERPNGIVLSPDETTLYVAD
                     TAKEHVRSFAVAPDGVATGGDVFADLTSDLVGGPDGMAVDVFGDIFVTGGGGVRVVTP
                     AGDLLGTIMVPEQATNCTFGDPDGMSLFITAQARVYRIRLKVKGLGLP"
     gene            2135..>3098
                     /locus_tag="VGB85_27430"
     CDS             2135..>3098
                     /locus_tag="VGB85_27430"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEY0137860.1"
                     /translation="MLAEATIIRRALALLHKRNLVLSIHDPSFPGAPGEDIGRGSPYT
                     RGGREFIEFIAGLGFTGLQFGPQGQTSPDNKSPYDGTIFARNLLSVDLLALAHDPRWA
                     GILPPESATRLTAIAPADGRRVAHRQVHTAMMDALDEAYAGFVARRAALDVLAPPLRA
                     ATERLISDFAAFRTEHHGWLERDTLFTTLAELHGTDDWRLWPPQDSRLYCPAPEEAAA
                     EDRRRARLLVTHAPALERHAFQQFIAHAQHDDLRLLTERLGLKLFGDLQIGLSDQDRW
                     SYRALFLPGYHMGAPPSRTNPDGQPWNYPVLDPAQYYNPDRTPGP"
BASE COUNT          509 a         1267 c          900 g          422 t
ORIGIN      
        1 tcgaaggtcg gctcgtgccc gaggatccgc atcaccgccg acgcgaaatt gcgggcgtac
       61 gacggcgtat gaaacccgac caggtccgcc ccgagcatcc cgcgcagcac ctccgcccgc
      121 cacggcagca tgcgaaacac ctccgacgcc gggaacggga tgtgcaagaa aaaccctatc
      181 ttcgcctcgg gcaggcgctc gcgcagcagc tgcggcaaca gcaacagctg atagtcctgc
      241 acccagatca cgtcgcccgg ctcgtacttc tcggccacgg cgtcggcgaa gcgcgcgttg
      301 acccgcatgt acgagtccca cccggtcgag tcgagcggca cccggtcgag caggtaatga
      361 aacagcggcc acaggacccc atttgaaaag tcctcgtagt agctgcgcac ctcctcggcg
      421 gtcagctgga ccggcaccgt gcgcaacttc tccagctcgc gcagcgcccc cgcctgctcc
      481 tcttcaccga gccccgagag gtcgcccggc cagccgatcc acagcccctg cgagcgctcg
      541 aacggcccct tgagccccgt cgccagcccg cccatgctgg gcgtcacgac cgccttcccg
      601 tcggccacgc tcacggtcac gggcaagcga ttggacacca cgatcaggcg tgacattgcc
      661 gcaagtctgg acgctcctct ggaatgagca agccgcgccc gccgcgcgcc gtggcgatcg
      721 acctcggcgc acgccccccg gagtcggcgc agatgcaacg gacggccgtc ggtcgtttcg
      781 acatcaccgg agcgatgcat acgacgtgac cacatcgatc ctcgggctgc gcgtggtcgt
      841 ccgctggggg cgagggtcca cgccatggac ttcgcgccgt gatcgttcat gacaacggca
      901 cgacgctggc cggccccacg cccgcgtgtt agacgggccc atgtcctcgc gcgagatctg
      961 gcttgcttcg ctcctcctga ccgcctgcgg cggctctccc tccgacacca ccggcaccac
     1021 cggcaccgag gcgaccggag cgaccagcac cggcgtcgag accactggca ccggcgagcc
     1081 cacgaccacg accacgatga cgacgcccga cacgaccacg gacacgccga ccggcccgag
     1141 caccaccgac acgaccaccg acaccaccgg cggctcgacc accggcggca ccaccggttc
     1201 gaccaccggt tcgaccacgg acacgaccac cgacccggtc gacccgccgg cgagcctcga
     1261 ggacgtcgtc gacggtgagc tcgagatgat cgccgacggt caccagttca ccgagggtcc
     1321 ggtgtggagc ctcgagggcc acctgctcta cagcgacatc cccgcgaaca cgatcttcaa
     1381 gtgggtcgag ggcggcgcat cggagccgtt tatcaccccc agcggcaact ccaacggcct
     1441 cgccttcgat tcgatgggtc gcctgctggc cgccgagcac ggcaatcgcc ggatctcccg
     1501 ccgtgtgctc ggcgaggacg cccagccggt cgccgacggc ttcgagggca agcagctcaa
     1561 cagccccaac gacctcatca tccgctccga cggcacgatc tacttcaccg acccgccgta
     1621 cggcatccag cccaatcaac aggagcagcc cttcaacggc gtgttccgcg tcgacacgca
     1681 gggccagctc tcgctcgtct ccgacgactt cgagcgcccc aacggcatcg tgctctcgcc
     1741 cgacgagacc accctctacg tcgccgacac cgccaaggag cacgtgcgca gcttcgccgt
     1801 cgcgcccgac ggcgtcgcca ctggcggtga tgtgttcgcc gacctcacca gcgacctcgt
     1861 cggcggcccc gacggcatgg ccgtcgacgt gttcggcgac atcttcgtca ccggcggcgg
     1921 cggcgtccgc gtcgtcacgc ccgcgggcga cctgctcggc acgatcatgg tccccgagca
     1981 ggcgaccaac tgcaccttcg gcgaccccga cggcatgtct ctgttcatca ccgcccaggc
     2041 ccgggtctac cgcatccgcc tcaaggtcaa gggtctcggc ctgccctgag ccgcgcttga
     2101 cacctgcggg actgccccgc aaagtctcgc gccgatgctc gccgaggcca cgatcatccg
     2161 ccgtgccctg gcgctgttac acaagcgaaa cctcgtcctc tcgatccacg acccgagctt
     2221 cccgggcgcg cctggtgagg acatcggccg cggctcgccg tacacccgag gtggtcgcga
     2281 gttcatcgag ttcatcgccg gcctcggctt caccggcctg cagttcggcc cgcagggcca
     2341 gacctcgccc gacaacaaga gcccctacga cggcacgatc ttcgcgcgca acctcctctc
     2401 cgtcgacctg ctcgcgctcg cccacgaccc gcgctgggcc ggcatcttgc cccccgagtc
     2461 cgccacccgc ctgaccgcga tcgcccccgc cgacggccgc cgcgtcgcgc atcgccaggt
     2521 ccacaccgcg atgatggacg cgctcgacga ggcctacgcc ggctttgtcg cccgccgcgc
     2581 cgcgctcgac gtgctcgccc ccccgctgcg cgccgccacc gagcgactga tctccgactt
     2641 cgccgcgttt cgaaccgagc accacggctg gctcgagcgc gacaccctct tcaccaccct
     2701 cgccgagctg cacggcaccg acgactggcg cctgtggccg ccgcaggaca gccgcctcta
     2761 ctgccctgcg cccgaggagg ccgccgccga ggaccgccgt cgcgcgcgtc tgctcgtcac
     2821 ccacgcgccc gcgctcgagc gccacgcctt ccagcaattc atcgcccacg cgcagcacga
     2881 cgacctgcgc ctgctcaccg aacgcctcgg cctcaagctg ttcggcgacc tgcaaatagg
     2941 tctctcggac caggaccgct ggagctaccg cgccctgttc ctgcccggct atcacatggg
     3001 cgccccgccg agccgcacca accccgacgg ccagccctgg aactaccccg tcctcgaccc
     3061 cgcgcaatac tacaaccccg accgcacccc cggacctg
//
LOCUS       DASWNJ010001652         6880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_169226, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001652 DASWNJ010000000
VERSION     DASWNJ010001652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6880
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169226"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VGB85_27435"
     CDS             complement(<1..637)
                     /locus_tag="VGB85_27435"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0137861.1"
                     /translation="MSVTPEAEATLSADSTATLKARVVELERELRMAIGVIDALPQGV
                     FWKDADLRLRGCNKVGRQVVGITNDMQFIGLTDFEMPYSREQAEAFVADDREVMARRE
                     ARPPVITSAQGFQGETRWVEHTKAPIFGDSDELLGVVGIFRDITDQKLAEQATAAEQR
                     AAMLRMSTPLLPVADGVIVLPLIGSLDPDRAEQVMQALLAGVVEHRATTAIL"
     gene            complement(723..1586)
                     /locus_tag="VGB85_27440"
     CDS             complement(723..1586)
                     /locus_tag="VGB85_27440"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0137862.1"
                     /translation="MPAEPATDAAPADALAASEARAARLEQQVRVMQGVLDALPYGLF
                     WKDLELVYRGCNKLGFAATGLSGSEREKFLGRTDFDMWQREQAEAFTADDREVLARRE
                     AKPHIVEAVRDATGATRWVETFKAPVFDAAGELLGVVGTFRDISEQKQAEQASAAEQR
                     EAMLRISTPLLPVADGVVVLPLIGELDPERAEQVMHTLLSGVAEHRATTAILDITGLH
                     TIDATAAEALVRAARAIRLLGAEAIVTGVRPAVAQTLVALGAELGDLVVLGDLKAGIR
                     HALARRAGRSD"
     gene            1732..3537
                     /locus_tag="VGB85_27445"
     CDS             1732..3537
                     /locus_tag="VGB85_27445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137863.1"
                     /translation="MGSLLLACGGGSAETGEGTSTGSSGAIDPTTTTTQSTTTTESAE
                     STEPTGPTGATDLTASATTGTTESTGSTGTTGTPTTGGITTGEDTLDTTSGSTSTGGA
                     CDGGCAPDFHDLDGDPGTGECGCEYACVKAGESDEIDAEFTDDNCDGSDGVVADCVFV
                     SVSLGDDAGDGGRDAPRKTIAAAQALAKQDGRPAVCLSGEVYATSVTMISGVHILGGF
                     DALDPDFKFRRTPGVVTTITATGTVVLADAILAETWLAGVSIHALSDPAPGASTHAVH
                     LLGGLKPLVLRHDVITADPGQPGPAGASGQPMNAAPAGAAGMHGCSGCMNLGKGGAAG
                     QCLAPGGKGGDGGFGSAAGMSGAAGTGGTPGGIGGAAVACFAGGKPGAAGAAGQTGAS
                     GAPGGGGGSIGSVMAGMYVAGGGTAGLPGAAAAAGGGGGGGGGGGADNFCTPDLGAGG
                     GAGGCGGQGGAPGQGGSSGGGSFAIFATGGSIVVDHNTIVAGAGGAGGVGGNGGPGQA
                     GGAGGKGGLAADDGGSGGNGGTGGKGGSGGPGGGGGGGPSACLARSQDALFVFEMNDC
                     VVGVSAPGGAGGTNPDNVMGTPGKNGQAAPDLLVN"
     gene            complement(3604..4179)
                     /locus_tag="VGB85_27450"
     CDS             complement(3604..4179)
                     /locus_tag="VGB85_27450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137864.1"
                     /translation="MCGDGIKDGDEECDDANEVEGDGCLSDCTKEWFVFVTSLPPLTS
                     DIKGLTGTDYQCRHRATLMFLPNGERYRAWISTSSEQAVDRLYHARGPYKLVNGLRVA
                     ASWDALIAGPLEHPINVTEMSETNHALVFTGTQPDGTRAPNSTHCDDWTDNDGLNQAW
                     YGDSDSKNKDWTFALEQGCGGGAALYCFEQP"
     gene            5877..>6880
                     /locus_tag="VGB85_27455"
     CDS             5877..>6880
                     /locus_tag="VGB85_27455"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0137865.1"
                     /translation="MVFHLRWSATFAVVFGLAACPGGDGDETAGTDTGASDDTNITTP
                     TGDFDPNPAYRGVLSGTVLGPSLHALDAGNIARARSDAECCQDYAAGSHDDSMLRIVF
                     GAANVGLTLFSDRADEVHNVGAGIDDLKIGDLNGDDRNDIIALRNDGNVAVLLGIDSP
                     PPAGPFLDELTQFSATAGNAVGARSLALGDLDCDGLTDVVVVAPTSDSVNLLRNDNAG
                     GFIAPVAFYMGVGSAPQEVVVGDFDEDGDDDVASSNSDGTMAVRLNMCSGNFDTSTSY
                     TVFLNLPSTAGMTIATGRICRGADTAASLALVVGFRDKVRVICGDGTGDFSLMGEEPI
                     "
BASE COUNT         1057 a         2229 c         2426 g         1168 t
ORIGIN      
        1 cgaggatcgc cgtggtcgcg cggtgctcga cgacacctgc gagcagggcc tgcatcacct
       61 gctcggcgcg gtcggggtcg agcgagccga tcagcggcag cacgatcacg ccgtcggcga
      121 ccggcagcag cggcgtcgac atccgcagca tcgccgcccg ctgctcggcc gccgtcgcct
      181 gttcggcgag cttctgatcg gtgatgtcgc ggaagatccc gaccaccccg agcagctcgt
      241 ccgagtcccc gaagatcggc gccttggtgt gctcgaccca gcgagtctcg ccctggaagc
      301 cctgcgccga cgtgatgacc ggcggccggg cctcgcggcg cgccatcacc tcgcggtcgt
      361 cggcgacgaa ggcctcggcc tgctcgcggc tatacggcat ctcgaagtcg gtgaggccga
      421 tgaactgcat atcgttcgtt atgccgacca cctggcgccc gaccttgtta cagccgcgaa
      481 ggcggaggtc ggcgtctttc cagaacaccc cctgcggcag cgcatcgatg acgccgatcg
      541 ccatccgtag ctcgcgttcc agctcgacca cccgggcctt gagggtcgcg gtcgaatcgg
      601 cagacagggt ggcctcggcc tcgggggtta cggacatcat ccgagcttat gccagcgcta
      661 cctcgcctgc cgagcgtcct ttgtcacagg tgcacgcgcg gggccgcgcg acgggtttgg
      721 gctcagtcgg atcggccggc tcgtcgcgcg agcgcgtggc gaatgccagc cttgaggtcg
      781 ccgagcacga cgaggtcacc gagctcggcg ccgagggcga cgagcgtctg ggcgaccgcc
      841 ggccgcacac cggtgacgat cgcctcggcg ccgagcaggc ggatggcccg ggcagcgcgc
      901 acgagcgcct cggcggccgt ggcgtcgatc gtgtgcaggc cggtgatgtc gaggatcgcc
      961 gtcgtcgcgc ggtgctcggc cacgccggac agcagcgtgt gcatcacctg ttcggcgcgc
     1021 tccgggtcga gctcgccgat cagcggcaag acgaccacgc cgtcggcgac cggcaacagc
     1081 ggcgtcgaga tccgcagcat cgcctcgcgc tgctccgccg cgctcgcctg ctcggcctgc
     1141 ttctgctcgc tgatgtcgcg gaaggtcccg accacgccga gcagctcgcc cgcggcgtcg
     1201 aacaccggcg ccttgaaggt ctcgacccag cgcgtcgcgc cggtcgcgtc gcgcaccgcc
     1261 tcgacgatgt gcggcttggc ctcgcggcgc gccagcacct cgcggtcgtc cgccgtgaac
     1321 gcctcggcct gctcgcgctg ccacatgtcg aagtcggtgc gcccgaggaa cttctcgcgc
     1381 tcgctcccgg agaggccggt cgccgcgaac ccgagcttgt tgcagccgcg atagacgagc
     1441 tcgaggtcct tccagaacag cccgtacggc agcgcgtcga ggacgccctg catcactcgc
     1501 acctgctgct cgagcctcgc ggcgcgggcc tcggaggcgg cgagcgcgtc ggcgggggcg
     1561 gcatcggtcg cgggctcggc gggcatgcgg cacagggtac gcgagggccc ccgacccgcc
     1621 gaggtcgtcg gtcgcgggca catcagcgcg caagcgcgca atcgcggctt gcaagtcgac
     1681 gaggcccgtg cgttccatgg ctggatgtca cgtgcgagtc gaggttggtg ggtggggtcg
     1741 ctgctcctgg cgtgtggtgg aggatcggcg gagaccggcg agggcacgag caccgggtcc
     1801 agcggcgcga tcgatccgac gacgacgacg acccagtcga cgacgacgac agagtcggcg
     1861 gagtcgacgg agccgaccgg gccgacgggc gcgacggatc tgacggcgag cgcgacgacc
     1921 gggacgacgg agagcactgg cagcacgggc acgaccggca cgcccaccac cggagggatc
     1981 accaccggcg aggacacgct cgacacgacg agcgggagca cgagcacggg tggtgcgtgc
     2041 gacggcggct gcgcgccgga cttccacgac ctcgacggtg atccggggac cggcgagtgc
     2101 ggctgcgagt atgcctgcgt caaggccggc gagtccgacg agatcgatgc ggagttcacc
     2161 gatgacaact gcgacggctc cgacggcgtc gtggccgact gcgtgttcgt gtcggtctcg
     2221 ctcggcgacg acgccggcga cggtggtcgc gacgcgccgc gcaagacgat cgccgcggcg
     2281 caggcgctgg cgaagcagga cgggaggccg gccgtatgtc tgtccggcga ggtctacgcg
     2341 acgagcgtga cgatgatctc cggcgtgcac atcctcggcg gctttgatgc cctcgacccc
     2401 gacttcaagt tccgccgcac gccgggcgtg gtcaccacga tcaccgcgac cggcacggtc
     2461 gtcctcgccg atgcgatcct cgcggagacc tggctcgcgg gcgtctcgat ccacgcgctg
     2521 tccgaccccg cgcctggcgc gagcacccac gcggtgcacc tgctcggcgg gctcaagccg
     2581 ctcgtgctgc gtcacgacgt gatcacggcg gacccggggc agcccggtcc ggcgggggcg
     2641 tcgggccagc cgatgaacgc cgcgccggca ggtgcggccg gcatgcacgg ctgtagcggc
     2701 tgcatgaacc ttggcaaggg gggggcggcc gggcaatgcc tcgcgcccgg cggcaagggt
     2761 ggggacggcg gcttcggctc ggccgccggg atgagcggag cggccggaac cggcggcacg
     2821 cccggcggca tcgggggcgc ggcagtcgcg tgcttcgccg gtggcaagcc gggagcggcc
     2881 ggtgcggccg ggcaaaccgg ggcatcgggc gcgccggggg gcggtggcgg gtcgatcggc
     2941 agcgtcatgg ccggcatgta tgtcgcgggt ggcggcacgg ccggcctgcc gggagccgca
     3001 gccgcggccg gcggtggagg aggtggcggc ggcggtggag gggccgacaa tttctgtacg
     3061 ccggacctcg gcgccggcgg tggtgccggg gggtgcggcg ggcagggcgg cgcgccgggc
     3121 cagggtggta gcagcggtgg cgggagcttc gcgatcttcg ccaccggcgg ctcgatcgtc
     3181 gtcgaccaca acacgatcgt cgccggcgcc ggcggtgccg gtggtgtcgg gggcaacggt
     3241 ggacccgggc aagccggcgg cgccggtggc aagggcgggc tggcggccga cgacggcggc
     3301 agcggcggca atggaggcac cggcggcaag ggtgggtcgg gcgggcccgg tgggggcggc
     3361 ggcggaggcc cgagcgcttg cctggcacgc agccaggacg ctctgttcgt gttcgagatg
     3421 aacgactgcg tcgtcggcgt ctcggcgccc ggcggggccg gtggcaccaa ccctgacaac
     3481 gtgatgggca cgccgggcaa gaacggccag gccgcgcccg acctcctggt caactgagcg
     3541 gctgcgtgcc ccgggctcga gcgggcgcgc cgtgtctgcg acccggtgcg cttcgagccg
     3601 cgatcacggc tgctcgaagc agtacagggc cgcgccaccg ccacagccct gctcgagcgc
     3661 gaaggtccag tccttgtttt tcgagtcgct gtcgccgtac caggcctggt tgaggccgtc
     3721 gttgtcggtc cagtcgtcgc agtgggtcga gttgggcgcg cgcgtgccgt cgggctgggt
     3781 gccggtgaac accagcgcgt ggttggtctc actcatttcg gtgacgttga tcgggtgctc
     3841 gagcggcccg gcgatcagcg cgtcccagct ggcggcgaca cgcagaccat tgacgagctt
     3901 gtacgggcca cgggcgtgat aaaggcgatc gacggcctgc tcgctgctgg tgctgatcca
     3961 ggcccggtag cgctcgccgt tgggcaggaa catcagcgtc gcgcggtggc ggcactggta
     4021 gtcggtgccg gtcaggccct tgatgtcact cgtgaggggc ggaaggctgg tgacgaacac
     4081 gaaccactcc ttcgtgcagt cgctcaggca gccgtcgccc tcgacctcgt tggcgtcatc
     4141 gcactcctcg tcgccgtcct tgatgccgtc gccgcacacc ggcgtgggat ccgtggtctg
     4201 gcctccgctc gtgtcggcgt ccgtcgtgtc ggtgctcgcc ggatcggggc cgctagatcc
     4261 ctcgctgccg gccgcggacg cggcatggcc ggtgtccgag ctgccgctcg tgtcggtgtc
     4321 gggggcaact ccgcggtcgt cgacgacgtc ccggtcgagc cgctcgtgtc ttcgggcatc
     4381 agcatcccct cgatgactgt gcccaccgcg cgcttaccgc tgcgctgcgc gctgctcacc
     4441 gggccgctcc gtactcaccc ccgcgcccaa attcccacct caagcgagcg cacggcgtct
     4501 gatcggccta gtccgtattt gagaattcgc gcccgaactc ccggttgacg tgggacatta
     4561 cttgcctcac cctcgaacga gggccagggc ttgatgaatc tccgactcca acgaacagct
     4621 ttttgggccg gtctgcctct gttcacctcg accccggcat ggagcggtga agcgaacgta
     4681 gcgagccatc gctctcccgc ccaaccgatc cgcgacggct tcactgtgga gccatgtggc
     4741 tggccttcga caatcacttt gataacacca aataacggca cgactgagcg gtgcaccggc
     4801 gtgtatatcg gtggacgtgt tgtggtcaca gccgcccact gcattcccgg tggctacttc
     4861 gtgcccacga actgtatgga ggacgcagac tgcccccaag tcgacgcctt cgggttgcct
     4921 cagccgttgg agtgctcact cggacagtgc gagagttctg atccaaaccg ttcgaatacg
     4981 gtcaaggaag tgctcttcgg agaatcctat cccgcgtatt tgacagatgg ccacatacgt
     5041 aagtccatcc cggtacatta ctgcgaacgt agagtcacgg gagaggacat aaacgaccct
     5101 gaagatttcg cctattgtct cctggtccaa cgaccaaatc tccgaccggt ccctgtgatg
     5161 atggactgtg aagccgatca attcttgcag cagggtgaag atgtggtcgc ggtagggttc
     5221 ggacgctctg tggcagcgga tgtcaatagc ggcggcacca agcggttcgc gtcatcgacg
     5281 ttgacaagct catcgacggc gaatgccaca acaatgagtt ctttctcgga ctggacgaac
     5341 gctggaggta caacccctag cggtccattg agcggggatt cgggcgggcc cctcatgctg
     5401 caacttccgg acggcacgtg gcgagtcgtc ggactcgccg tcacggacct gcccgagtac
     5461 acgacgatct ggcataatat tcgctggatg ttcaccacag caaatgactc taatctaatc
     5521 aacgacctcg ccgaggtgct gccttgccac actccagatg gggactggtc accgacagcg
     5581 gcctgcactg ggttcccaat gtcacccgac atgggcgcgg gagattgggc gcgtggaccg
     5641 cgcgtgtgtg actccacgga tattggcgac ttcagcgcca cgtgcggtgc gcccttcgct
     5701 ctggcgctgg taccgccgcg accgtctcat gacccgtcgt tcaatttctc caggggtcaa
     5761 ggcaatactg ctgttgcccc agaatctatc cgggccatac tattcacggg cggcctctgc
     5821 gccattctcc tgggggcatt ttggttacga cgccgtacgt acggatgatc tacgcggtgg
     5881 tcttccacct ccgttggagc gcgaccttcg ccgttgtgtt cggtttagca gcctgcccgg
     5941 gtggggatgg cgatgagaca gccgggacgg acaccggtgc gagtgacgac acgaacataa
     6001 ctaccccgac aggggacttc gatccgaatc cggcttatcg cggggtcctg tccgggacag
     6061 tcttggggcc ctcgctccac gcgctcgatg ccggcaatat tgcgcgcgcc aggtccgatg
     6121 ccgagtgttg ccaggattat gccgctggta gccatgacga cagcatgctg cggatcgtct
     6181 tcggagccgc gaatgtcggc ctgacgctgt tctcggaccg agcggacgag gttcataacg
     6241 ttggtgcggg tatcgacgat ctaaagatcg gggatctaaa cggcgatgat cgaaacgata
     6301 taatcgcgtt gcgtaacgac ggcaatgtgg ccgtgttgct cggcatcgat tctccaccgc
     6361 ctgctggccc attccttgat gaactcacgc aattctcggc gaccgcgggg aacgctgtcg
     6421 gtgcgcgctc gttggctctc ggcgacttgg attgtgatgg gctaacagac gtggttgtgg
     6481 ttgctccgac tagtgactcc gtaaacctgc taaggaatga taatgctggt ggattcattg
     6541 ctccggtcgc tttttatatg ggcgtcggga gcgctcctca ggaggtcgtt gtaggtgact
     6601 tcgacgaaga tggcgatgac gatgttgcaa gctcgaatag cgatggcacc atggctgtcc
     6661 gcctgaacat gtgcagtggg aatttcgata cctcgacgtc ctataccgtg tttttgaact
     6721 taccctcgac agcgggaatg accatcgcta caggccgaat ctgccgcggc gccgacaccg
     6781 ccgcatcctt ggctctcgtc gttggttttc gggataaggt gcgggtgatc tgcggtgatg
     6841 gtacaggcga tttctcgttg atgggcgagg agccaattag
//
LOCUS       DASWNJ010001653         2475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_100411, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001653 DASWNJ010000000
VERSION     DASWNJ010001653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100411"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VGB85_27460"
     CDS             complement(<1..333)
                     /locus_tag="VGB85_27460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137866.1"
                     /translation="MAVGLATGCQAQVVMDPEVGAEEGADEAPDDPVPDVPTSGPVTT
                     GPVDPSTGGADESGGSGEAGGSGSASTTVPPGSCGDGVVDEGEGCDLGYAQNNDQGPC
                     TKACTAAYC"
     gene            complement(474..1154)
                     /locus_tag="VGB85_27465"
     CDS             complement(474..1154)
                     /locus_tag="VGB85_27465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY0137867.1"
                     /translation="MRRPPDPRTDEELNAALLAGDKSAGAVLYDRHFAPLRGYFINKV
                     ADPSVWDDLVHQTFEVLLGKPVNFKGDSTYRSYVFGVAHNILRTHYRAQRLQSSRRDD
                     SIEQIEELSVSDLGPGVSTLVTNQADAQRLIEALRKIPIKYQSVFEMYYWQDLPASEI
                     ARTCACPIGTVRGRLRLAKKALLEKLALEGGSYGELLRGFRSVDAWSREVRAELGTDD
                     DDDDEGVA"
     gene            1282..>2475
                     /locus_tag="VGB85_27470"
     CDS             1282..>2475
                     /locus_tag="VGB85_27470"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0137868.1"
                     /translation="MPTPDLAEPPPARLTGSARRPSLAATLPDVKHAGRNAGIASAFV
                     YLSMRAAFFPDGPQAALAIGGRYTLLKKLGEGSMGVVFVAFDSELNRKVAIKVVRSPG
                     VAQDRLVQEARSLAQLNDPHVVQVYEARKHEDEVYLAMEYVEGCSLDVWVAREKPDLP
                     VLLGKLIEAGHGLAAAHAGHFVHRDIKPANILVGDDGRVRVADFGLAWADAHNPGTLP
                     EIGAGAVDSTISVGAGTPAYMAPEQLRGEAIDRRSDLFSFCVVVWEAAFGQRPFLGET
                     VARISLAIDDGEITAPPVRPRGALARLERTLRRGLAPAPADRHPDMHALLAELGRIRT
                     PRSLLPWALAASAAVIVGLAVPYAQANACERLGAEIDEIWRSEDGPALAATIPAVTPG
                     AAGRLV"
BASE COUNT          359 a          942 c          793 g          381 t
ORIGIN      
        1 acaataggcc gccgtacagg ccttcgtgca gggcccctga tcattgttct gcgcgtagcc
       61 cagatcgcag ccctcgcctt cgtcgaccac cccgtcgccg caggaccccg gcgggaccgt
      121 cgtcgacgcg ctgcccgagc cgcccgcctc gcccgagcca ccgctctcgt cggcgccgcc
      181 ggtgctcgga tcgaccgggc cggtcgtcac cggccccgac gtcggcacgt ccggcacggg
      241 gtcgtccggc gcctcgtcgg caccctcctc ggccccgacc tccggatcca tcacgacctg
      301 cgcctggcag cctgtggcta gcccgaccgc caaagtcacc aatatcgcaa gcaagcctcc
      361 tggcgcgctc aacatgcacg catcatggca gccacgcgat cccgtgtcaa tccgcccgct
      421 ctctgtaata actgtatgtg gccttaccaa ttcggcacag gccaatgttg gtctcaggcc
      481 acaccctcat catcatcatc gtcgtcggtc ccgagctcgg cccggacctc gcgcgaccag
      541 gcatcgacgc tccgaaagcc gcgcaggagc tcgccgtagc tgccgccttc cagcgccagc
      601 ttctcgagca gcgctttctt ggcgaggcgc aggcgcccgc ggaccgtccc gatcgggcag
      661 gcgcaggtgc gggcgatctc gctggcgggc aggtcttgcc agtaatacat ctcgaacacg
      721 gattgatatt tgatagggat cttgcgtaat gcctcgatca atcgctgcgc gtcggcctgg
      781 ttggtgacca gcgtcgacac gccgggcccg aggtcggaca ccgatagctc ctcgatctgc
      841 tcgatcgagt cgtcgcggcg gctgctctgc aggcgctggg cacgatagtg ggtgcgcagg
      901 atattgtggg cgacgccgaa cacgtacgag cgataggtcg agtcgccctt gaagttcacc
      961 ggcttgccga gcagcacctc gaaggtctga tgcaccaggt cgtcccagac cgagggatcc
     1021 gcgaccttgt tgataaaata tccgcgcagc ggcgcgaagt ggcggtcgta caacacggcg
     1081 ccggccgact tgtcgcccgc cagcagcgcg gcatttaatt cctcgtccgt ccgggggtcg
     1141 ggtggtcgtc gcatggaaac atcgtattta ataccaggat cgagcgctgc gcgggcgtgc
     1201 actcggcgct ggcctgatgc cgcccgatcg gtgatcgtcc gcgggccgtc ccgacactcc
     1261 ctcacgcacc cgcgttcgac catgccaacc ccggacctcg ccgagccacc ccccgcgcgc
     1321 ctgaccggca gcgcccgccg tccctcgctg gccgccacgc tccccgacgt caagcacgcc
     1381 ggccgcaacg ccggcattgc gtccgcattt gtatacctgt cgatgcgcgc cgcgttcttc
     1441 cccgacggcc cccaggcggc gctggcgatc ggcggccgct acacgctcct gaagaagctc
     1501 ggcgagggca gcatgggcgt cgtcttcgtc gccttcgaca gcgagctcaa tcgcaaggtc
     1561 gcgatcaagg tcgtgcggtc gcccggcgtc gcgcaggacc ggctggtgca ggaggcgcgc
     1621 agcctcgccc agctcaacga cccccacgtc gtccaggtct acgaggcacg caagcacgag
     1681 gacgaggtct atctcgccat ggagtacgtc gagggctgct cgctcgacgt ctgggtcgcg
     1741 cgcgagaagc cggacctgcc ggtgctgctc ggcaagctga tcgaggccgg tcatggtctc
     1801 gccgccgcgc acgccgggca cttcgtccat cgtgacatca agcccgccaa catcctcgtc
     1861 ggcgacgacg gccgcgtccg cgtcgccgac ttcgggctcg cgtgggccga cgcgcacaac
     1921 cccggcaccc tccccgagat cggcgccggc gcggtcgaca gcacgatctc ggtcggcgcc
     1981 gggaccccgg cgtacatggc cccggagcag ctccgcggcg aggcgatcga tcgccgcagc
     2041 gacctgttta gcttctgcgt cgtcgtctgg gaggcggcgt tcggccagcg tcccttcctc
     2101 ggcgagaccg tcgcgcgcat cagcctggcg atcgacgacg gcgagatcac ggcgccgccg
     2161 gtccgcccgc gcggcgccct ggcccggctc gagcggaccc tccggcgtgg cctcgccccc
     2221 gcgcccgcgg accgccaccc cgacatgcac gcgctgctcg ccgagctcgg gcgcatccgt
     2281 acgccgcgct cgctcctgcc gtgggcgctg gcagcgagcg cggccgtcat cgtcggcctc
     2341 gcggtcccct acgcgcaggc caacgcctgc gagcgcctcg gcgccgagat cgacgagatc
     2401 tggcgcagcg aggacggccc ggccctggcc gccacgatcc ccgccgtcac gcccggcgcc
     2461 gcgggcaggc tcgtc
//
LOCUS       DASWNJ010001654         4915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_207368, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001654 DASWNJ010000000
VERSION     DASWNJ010001654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207368"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1183)
                     /locus_tag="VGB85_27475"
     CDS             complement(80..1183)
                     /locus_tag="VGB85_27475"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEY0137869.1"
                     /translation="MLRRHLALPVLLCAIGCGGGGEASSSGGSSETGASTTASTTAST
                     TPTTGDATASSGGTTGEPDPCVASPQALADCVDGAAYAEDLNFIADIRTPGTPHWQAV
                     QDLCADRLAEAGYEVILQDYGTGINVLGRRVGASKPNELVVVAAHYDHIADCHGADDN
                     ASGVAATLEIGRLLARAELERTVIIACWDEEEAGLIGSEAFVAAVPVEDTVVVNYNFD
                     MIGVRSDEMDSQRVPPGFEAVFPEQYAAIESRGFRGDFIAIVASSLAHEHALTFAGAT
                     GRIALASEVIEIPAGAENSPVFDDLRRSDHAAFWLAGIPALFITDTGEFRNDHYHCMK
                     GPDEVADLDLDFAVGVTKATVEAAAVTLGMQVQ"
     gene            1607..>4915
                     /locus_tag="VGB85_27480"
     CDS             1607..>4915
                     /locus_tag="VGB85_27480"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0137870.1"
                     /translation="MPAGESGAGLASGTLLGNRFELLAPMGRGGMGTVFRAIDRQTAR
                     EVAVKVISGETASFHESERFSREARILAELRHPGIVEYIAHGRTLAGRPFLAMEWLEG
                     EDLGQRLVRGPLDLHDALGLVRTVAQALAVLHRCGVVHRDLKPSNIFLREGRLERATL
                     LDFGIARTIAGTARLTVTGVVIGTPDYMAPEQASGQHDVGPAADIFSLGCVLHECLAG
                     EPPFSGRHVAAVLAKILFQEAPSLRARRPDVPEAVEAALRSMLAKDPERRPADATALL
                     GALARVEDLVTGDDATLAPRVALAHALADVEQRYVSVVIVGGGEQAPTNATLGPEELA
                     SRKLERRSLDESLARLGARTAWLADGSIVATLAHTGSANDQAAQAARCALVVHERWPS
                     AAIGLATGRGVLHERLPVGEAIDRAALLLRTPSDSTAGLVLVDDVSAGLLDARFVLEP
                     GDGAFVLMSEQSATREARRLLGKPTPCVGREQELAMLGAVLSTCTDESVARAVLVSGP
                     PGMGKSRLHFEFLRRLPTRGATPLVLSGRADPVGAGSPYGILTEALLAECGAVRAEAL
                     SVQQAALRRRLARHLPAADAPRVTEFLAELCGLPFVDEASPSLRAARRDPRLMNDQVS
                     LAFVDLVRAECRARPLLLVLEDLHWGDVLTVKLIDRALRELPDQPWMVLAFARPEVDE
                     LLPARWSDVVQEIRLGGLSKKASERLVHEVLGPSVPDETVARVVKQAGGNALHLEELI
                     RAVAEGKGEELPETVLAMLHMRLHQLAPEARRLLRAASIFGATFSRDSVVALSGADKD
                     DARVLLDALIRDEVVEHRRERRFADDDDYCFRHALLRDAAYATLLDADRALGHRLAGA
                     FLEQAGEHDPMVLAEHYRRGGEPERAVPLYARAAEQALLSNDLTSALARVERAIGCGA
                     SGEALGVVQGLAASAYFWRDEWAPGYEAAKVALAHLEPGGLRWCKACAHQFVFTSVGL
                     AKHEEFGELVGLFAGTAPAEAARAAFVEAASWLVTMFSLLGQRDATRGFLALMDRVCS
                     AIGGADVMAEGWQAQAHMFHARMLGEDPWLTQAHAERASAAFRSMSDQRNLAFIEAYR
                     GLAQADLGLPEAGEA"
BASE COUNT          640 a         1786 c         1778 g          711 t
ORIGIN      
        1 gccgccgccc cgccggcgcg tgttcggcaa gccgttccgc ccggcccgca agggcccgcg
       61 gccggagtga gcggcggcgc tactgcacct gcatcccgag cgtgacggcg gcggcctcga
      121 cggtcgcctt cgtcacgccg accgcgaagt cgaggtcgag gtcggcgacc tcgtcggggc
      181 ccttcatgca gtggtagtgg tcgttgcgga actcgccggt gtcggtgatg aacagcgcgg
      241 ggatgccggc cagccagaac gccgcgtggt cggagcggcg caggtcgtcg aacacggggc
      301 tgttctcggc gccggcgggg atctcgatca cctcggacgc gagcgcgatg cgcccggtgg
      361 cgccggcaaa ggtcagcgcg tgctcgtggg cgagcgagct ggcgacgatg gcgatgaagt
      421 cgccgcggaa cccacgcgac tcgatcgcgg cgtactgctc ggggaacacg gcctcgaagc
      481 ccgggggcac ccgctggctg tccatctcgt ccgagcggac cccgatcatg tcgaagttgt
      541 agttgacgac gacggtgtcc tcgaccggca cggcggcgac gaaggcctcg gagccgatca
      601 ggccggcctc ctcctcgtcc cagcaggcga tgatgacggt gcgctcgagc tcggcccgcg
      661 ccagcaggcg gccgatctcg agggtggccg cgacgccgct cgcgttgtcg tcggcgccgt
      721 gacagtccgc gatgtggtcg tagtgcgcgg cgacgacgac gagctcgttc ggcttgctgg
      781 caccgacccg gcgaccgagc acgttgatgc ccgtgccgta gtcctgcaag atcacctcgt
      841 agccggcctc cgccaggcgg tcggcgcaca ggtcctgcac cgcctgccag tgcggcgtgc
      901 ccggggtgcg aatgtcggcg atgaagttga ggtcctcggc gtaggccgca ccgtcgacgc
      961 agtcggcgag cgcctgcggt gacgcgacgc aggggtcggg ctcgccggtg gtgccgccac
     1021 tgctcgccgt tgcgtcgccg gtggtcgggg tcgtgctggc ggtagtgctg gcggtcgtgc
     1081 tcgcaccggt ctcgctcgag ccgccggagg agctcgcctc gccgccgcct ccgcagccga
     1141 tcgcacacag cagaacaggg agggccaggt gtcgccggag catgtcgggc gcaaagctac
     1201 cacgatctcg cgcgccgcga ccggcccgcg gctcgcaggc cgatgctgtc cccggggaca
     1261 gctcgccgcg tccacggccc gcggcgcggc acgtcgacga agatcgcggt tcaaggcgat
     1321 cgcggcgacg gtgtccgtat cgccgacgac gcagaccgcg agctactcgc gctgatcgag
     1381 ccgatgttgt cgtgtggcac acgtgccaca cgtgcgccgg cagtaccgat cgcgccgagc
     1441 gcgacgcgtc gccgcacgcg cacatggtct cgacatcggg gtcgccggaa cccgtgaggt
     1501 gccgaggccg cgaaacactc gggcccgtat cgccgcggga tcccccgctt gacgcgacgt
     1561 cgccgtagcc gataacgtga ctcgaatggt gctatcggcc gacagcatgc ccgctgggga
     1621 gagcggcgca gggctggcct ccgggacgct cctgggcaac cgcttcgagc tcctcgcacc
     1681 gatgggtcgc ggcgggatgg ggacggtctt tcgcgcgatc gatcgccaga ctgcacgtga
     1741 ggtcgccgtg aaggtgatct ccggggagac cgcgtccttc cacgagagcg agcggttctc
     1801 gcgcgaggca cggatcctcg ccgagcttcg ccaccccggc atcgtcgaat acatcgccca
     1861 tggtcggaca ctcgcaggtc gaccgttcct cgcgatggag tggctcgagg gggaggacct
     1921 cggccagcgg ctggtccgcg gtccgctgga cctccacgac gcgctgggcc tcgtccgtac
     1981 cgtcgcccag gcgctcgctg tcctgcaccg gtgcggcgtc gtccaccgcg acctcaagcc
     2041 gtcgaacatc ttcctccgcg aaggtcgact cgagcgcgcc acgctcctcg acttcggcat
     2101 cgcgcgaacc atcgccggca cggcgaggct caccgtcacg ggcgtggtga tcggtacgcc
     2161 cgactacatg gcgcccgagc aggcctcggg acagcacgat gtcggccccg ccgccgatat
     2221 cttctcgctc ggctgcgtgc tccacgaatg cctcgccggt gagccgccgt tctccggccg
     2281 ccatgtcgcc gcggtgctgg cgaagatcct gttccaggag gcgccttcgc tgcgcgcgcg
     2341 ccggccggac gtacccgagg cggtcgaggc cgcgctccga tcgatgctcg ccaaggaccc
     2401 cgagcgtcgg cctgcggacg ccaccgcgct gctcggcgcc ctggcgcgcg tcgaggacct
     2461 ggtgaccggc gacgacgcga ccctcgctcc gcgcgtcgcc ctggcgcacg cgctcgccga
     2521 cgtcgagcag cgctacgtca gcgtggtgat cgtcggcggc ggcgagcagg cgccgacgaa
     2581 cgcgacgctc ggcccggagg agctcgcgag ccgcaagctc gagcgacgct cgctcgacga
     2641 gagtctcgcg cgcctcgggg cgcgcactgc ctggctcgcc gacggatcga tcgtcgccac
     2701 gctcgcacat accggcagcg ccaacgatca ggcggcccag gccgcccgct gcgctctcgt
     2761 cgtccacgag cgctggccgt ccgccgcgat cgggctcgcc accggccgcg gcgtgctcca
     2821 cgagaggttg ccggtcggcg aggcgatcga cagggcggcg ctgttgctcc gcacgccgtc
     2881 ggactcgacc gccggcctgg tgctggtcga cgatgtcagc gcgggtctgc tcgacgctcg
     2941 cttcgtcctc gaaccaggag acggcgcctt cgtcctgatg agcgagcagt cggccacccg
     3001 ggaggcgagg cgcctgctgg gtaagcccac gccgtgcgtc ggccgcgagc aggagctggc
     3061 gatgctcggc gcggtgctct cgacatgcac cgacgaatcc gtcgcgcgcg ctgtcctcgt
     3121 atcggggccg ccagggatgg ggaagtctcg gctccacttc gagttcctcc gacgcctccc
     3181 gacccgcggc gcgacccccc tcgtgctctc cggtcgagcg gacccggtcg gcgcgggctc
     3241 tccctacggg atcctcaccg aggccctcct cgcggagtgc ggcgccgtgc gagcggaggc
     3301 gctgtcggtc cagcaggccg cgctgcgcag gcgcctcgcg cggcacctcc ccgctgccga
     3361 tgccccgcgc gtgaccgaat tcctggccga gctgtgcggg ctgccgttcg tggacgaggc
     3421 gagcccctcg ctccgcgcgg cccgtcgcga cccgcgcctc atgaacgacc aggtgagcct
     3481 ggccttcgtc gatctcgtgc gcgcagagtg ccgcgctcgg ccgctcctgc tcgtgctcga
     3541 agatctgcac tggggcgatg tcctgacggt gaagctgatc gaccgggccc tgcgcgagct
     3601 gcccgatcag ccgtggatgg tgctggcctt cgcgcgaccc gaggtcgacg agctgctccc
     3661 tgcccgctgg tccgacgtag tgcaggagat ccggctcggg ggactgagca agaaggcgag
     3721 cgagcggctc gtccatgagg tcctcggccc gagcgtcccc gacgagaccg tcgcgcgcgt
     3781 cgtcaagcag gccggcggca acgccctcca cctcgaggag ctcatccgtg cggtcgcgga
     3841 gggcaagggc gaggagctgc cggagacggt cctggcgatg ctgcacatgc ggctccacca
     3901 gctcgccccc gaggcgcggc gcctgttgcg tgcagcgagc atcttcggcg ccaccttctc
     3961 gcgcgacagc gtggtcgcgc tgtccggggc cgacaaggac gacgcgcgcg tgctgctcga
     4021 tgcgctgatc cgtgacgagg tcgtcgaaca ccgccgcgag cgccggttcg ccgacgacga
     4081 tgactactgc ttccgccacg cgctcctgcg cgacgccgcg tacgcgaccc tgctcgacgc
     4141 cgaccgcgcg ctcggtcacc gcctcgccgg cgcgttcctc gagcaggccg gcgagcacga
     4201 cccgatggtg ctcgccgagc actaccggcg cggcggtgag cccgagcggg ccgtgccgct
     4261 ctacgccagg gccgcggagc aggccttgct cagcaacgac ctcaccagcg cgctcgcgcg
     4321 ggtggagcgt gcgatcggct gcggcgcgag tggtgaggcg ctcggcgtcg tccagggcct
     4381 cgccgcctcg gcctacttct ggcgcgacga gtgggcgccc ggatacgagg ccgccaaggt
     4441 cgcgctggcg cacctcgagc ccggcggcct tcggtggtgc aaggcatgcg cgcatcagtt
     4501 cgtcttcacg agcgtcgggc tcgccaagca cgaggagttc ggcgagctcg tcggcttgtt
     4561 cgccggcacc gcgcccgccg aggcggcccg ggcggccttc gtcgaggccg cgtcgtggct
     4621 ggtcacgatg ttctctctcc tcggtcagcg ggacgcgacc cgcggcttcc tcgccctgat
     4681 ggatcgcgtg tgctcggcga tcggtggcgc cgacgtgatg gccgaggggt ggcaggcgca
     4741 ggcccacatg ttccacgcgc gcatgctcgg cgaagacccg tggctgacgc aggcccacgc
     4801 cgagcgggcg agcgccgcgt ttcgcagcat gtccgaccag cgcaacctgg cgttcatcga
     4861 ggcctatcgc ggcctggccc aggccgacct cggcctgccc gaagcgggcg aggcg
//
LOCUS       DASWNJ010001655         2423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_522993, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001655 DASWNJ010000000
VERSION     DASWNJ010001655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522993"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VGB85_27485"
     CDS             <1..735
                     /locus_tag="VGB85_27485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137871.1"
                     /translation="LGVTGCSARATGPVTPVVSAVPVAFTPAPATLPAAAPAPVQTRV
                     AGRHALGAHKTVQGTHQRNFYREDTRAALVVELAEDGGVTACRGLHSRSSHHTDHGTD
                     ARDLRDQQGYRGRWSQRDGELDLRFAPDAAVCPTTRAYTNLEPRPWHLRCTMVTPQEH
                     PQLASAALACRLINRGELMFSEEYGLELPGILPDPMILLGRGDGVEVEWSADSLVPPD
                     SAPEVEVRPAVKRIEAGSWELPAATP"
     gene            complement(745..1191)
                     /locus_tag="VGB85_27490"
     CDS             complement(745..1191)
                     /locus_tag="VGB85_27490"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEY0137872.1"
                     /translation="MHTAPRPSPAPTGPDSTDRLLAGSAYASSLLGLWLVVPLIIYCV
                     RREQSRFVAYHAMRAMILQCACVALGVLGLVLAMTLLHAPTTPGTVIELGSAVFLLFA
                     LLVPALHVVLAVIAMVLAFRGRTNRRALFARTTERILRRDAGVAIE"
     gene            complement(1232..>2423)
                     /locus_tag="VGB85_27495"
     CDS             complement(1232..>2423)
                     /locus_tag="VGB85_27495"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137873.1"
                     /translation="IGGSALGPQLLAYALGGRLHVLDNTDPDGIARTLAGLPSLAHTL
                     VLVTSKSGATVETRNGMLEVAAACTRAGLEFARQAVAITGIGSNLDLLAKNQGWRARF
                     PLWDWVGGRTSVLSPVGLVPAALLGLDWRGLLRGAAAMDVATREPDVRRNPAAMLAHA
                     WFALTEGHGTKAMVVLPYADRLVLLSRYLQQLVMESLGKREDRQGGEVWQGLTVYGNK
                     GSTDQHAYVQQLRDGLPDFFASFVRVLTSDAERGAIEVEPGVTSGDCLDGFYQGTRSA
                     LAERGRPSLTITLERLDAPTLGAVIALFERSVGLYAELIDINAYHQPGVEAGKLAARS
                     VLTLQHRFLRALAAEPRTVAELAETLASEPFAVWQLGRRLVLPGRVRGESLADPASAR
                     FAAV"
BASE COUNT          355 a          879 c          886 g          303 t
ORIGIN      
        1 ctcggcgtga ccggatgcag cgctcgcgcg acggggccgg tcacgccggt cgtctccgcg
       61 gtcccggtcg cgttcacgcc cgcgcccgcg accctgcccg ccgccgcgcc cgcgccggtg
      121 cagacccgcg tcgccgggcg ccacgcgctc ggcgcccaca agaccgtgca gggcacccac
      181 cagcgtaatt tttatcgaga ggacacccgg gcggccctcg tcgtcgagct cgcggaagat
      241 ggcggcgtga cggcctgccg cggtcttcac agtcggtcga gtcaccatac cgaccacggc
      301 accgatgctc gcgacctgcg cgaccagcag ggctatcgtg gccgctggtc ccagcgggac
      361 ggcgagctcg acctgcgctt cgcgcccgac gccgcggtgt gcccgacgac gcgcgcctac
      421 accaacctgg agccgaggcc ctggcacctg cgctgcacca tggtcacccc gcaggagcac
      481 ccgcagctgg cctccgcggc gctcgcgtgt cggctgatca accgcggcga attgatgttc
      541 agtgaggagt acgggctcga gctcccgggc atcctgccgg accctatgat cctgctcggc
      601 cgcggtgacg gggtcgaggt cgaatggagc gccgactcgc tggtgccacc ggacagcgcg
      661 cccgaggtgg aggtccgccc ggccgtgaaa cgcatcgagg ccggcagctg ggagctgccg
      721 gccgcgacgc cgtgaggacg gcgctcactc gatcgccacg cccgcgtcgc ggcgaaggat
      781 ccgctcggtg gtccgcgcga acagggcccg acggttggtc cggccgcgaa acgcgaggac
      841 catcgcgatc accgcgagca cgacgtgcag cgccggcacg aggagggcga acagcaagaa
      901 cacggcgctg ccgagctcga tcacggtccc gggcgtcgtg ggggcgtgca ggagggtcat
      961 cgcgagcacg aggccgagca cgccgagcgc cacgcaggcg cattgcagga tcatggcccg
     1021 catcgcgtga taggcgacaa agcgcgactg ctcgcgtcgc acgcaataga tgatcagcgg
     1081 cacgaccagc cacaggccca gcagggagct cgcgtacgcg gatccggcga gcagtcggtc
     1141 ggtcgaatcg gggccagtgg gggcggggga ggggcgcggg gcggtgtgca tcgcccgggt
     1201 tcggcacccg ggctcccgct gttgcgcccg atcacaccgc ggcgaagcgg gcgctggccg
     1261 ggtcggcgag cgactcgccg cgcacgcgtc cgggcagcac cagcctgcgc ccgagctgcc
     1321 acaccgcgaa gggctcggac gcgagggtct cggccagctc ggcgacggtc ctcggctcgg
     1381 ctgccagcgc acgcaggaat cgatgttgca gcgtcagcac gctgcgcgcc gcgagcttgc
     1441 cggcctcgac gccgggctgg tgatacgcgt tgatgtcgat cagctcggca tacaggccga
     1501 cgctgcgctc gaacagggcg atcacggcgc cgagggtcgg cgcgtcgagg cgctcgagcg
     1561 tgatcgtcag gctcgggcgc ccgcgctcgg cgagcgcgct gcgggtgccc tgatagaagc
     1621 cgtcgaggca gtcgcccgag gtcacgcccg gctcgacctc gatcgcgccg cgctcggcgt
     1681 cgctggtcag cacgcgcacg aagctggcga agaagtcggg caggccgtcg cgcagctgtt
     1741 gcacatacgc gtgctggtcg gtcgacccct tgttgccgta gaccgtcagc ccctgccaca
     1801 cctcgccgcc ctggcgatcc tcacgcttgc cgagcgactc catcaccagc tgttgcagat
     1861 agcgcgacag cagcaccaga cggtcggcgt agggcagcac gaccatcgcc ttcgtgccgt
     1921 gaccctccgt cagcgcgaac caggcatggg cgagcatcgc cgccgggttg cgccggacgt
     1981 ccggctcgcg cgtggcgacg tccatcgccg ccgcgccgcg tagcaatcct cgccagtcga
     2041 ggccgagcag cgccgccggc accaggccga ccggcgacag caccgaggtg cgcccgccga
     2101 cccagtccca cagcgggaag cgggcccgcc agccctgatt cttggccagc aggtcgaggt
     2161 tgctgccgat ccccgtgatc gccacggctt ggcgcgcgaa ctcgaggccc gcgcgcgtgc
     2221 acgcggcggc gacctcgagc atgccgttgc gcgtctcgac ggtcgcgccc gacttgctgg
     2281 tcacgagcac gagcgtgtgc gcgagcgagg gcaggccggc gagcgtgcgg gcgatgccgt
     2341 cggggtcggt gttgtcgagc acgtgcaggc gcccgccgag cgcgtaggcc agcagctggg
     2401 gcccgagcgc cgagccgccg atg
//
LOCUS       DASWNJ010001656         9920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_100567, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001656 DASWNJ010000000
VERSION     DASWNJ010001656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 9920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9920
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100567"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..7971)
                     /locus_tag="VGB85_27500"
     CDS             complement(214..7971)
                     /locus_tag="VGB85_27500"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0137874.1"
                     /translation="MATHPTDDQALAAALAAARSEPSKHEHWDLAEQLGEAHQQVDEV
                     AAAYREALTQKLPPDVVDELGRRAADFHETWLGDDNASLTDVLLRVLAAQPQAEWAFA
                     KLTSVYTLRERWSELLDLYDRTLAAPVAETRRLQLLEEAADIARDFASQADRAVGYMH
                     QLLTLRPGDERLEASLERLLERQERWRDLVALWKRRLQLQGLAANPGLQARIAEASLD
                     RLHDPAAALTEARALLQTDANDRGAAALLERLLVLEGADASVRAGALDLLRSTYEHAH
                     RPEEVIRVLEVALPGSEPATAIDLHREIAERLVAIDRPVPAMQHHVALLALAPDSLED
                     QRRLRHLAETTGDAAVHVQGLRAAADATTDVQRRVALLAEAADVQHDILHDHAAAIEL
                     YTRVLAEAAAPPATALRVARILEDLLAEAGRDAERLTVLERLGVLEDGAAQAQSLGDA
                     ARLAEQLGEPERALEVWRARLALDGEDPEALRHAIRLFEQLGRWAELVETLRQRAGGP
                     VAPHQRRADLTRVAALEAAQLDDPAAAISTWESVQQEFGEDRETVEAMAALLARVGRW
                     QALAELLTRAAGHETSQVADLSTRLGDVYRTQLGETARAVACYDAALSVDPGHAGARA
                     GLVAALEDPAARAGAVELLGKLHRKREEWPELAGIVEARLAITDDPRKQAEILRETAT
                     LQEQHVGDPRAALELLRRAFPRTPRDRELERDIVRLAEQTGAWQLAVDAYREAVATLV
                     GDPRRSAELRLAEARTLEHKLGEHDPALAAYNAVLAIEPGDADALRGAVRMAARVTRG
                     DAAAAALVAQVGADGKLDASLLTLVESEAAQFGGWQGLTLALEAALAEARLPATPAAF
                     LEARVALWHRDHRDDAAAARSALQRSLAIVPDDVGRLEMLAQLQRAAPDTQLIDTLLA
                     LAGQREDDFDEHYEASELALGLDGDTPRTRDILRRLQTRASALWNRGQPAKGKRTPEE
                     SVAWAVQQLGRLHDQAGERREAAELLGRSASLPFAPATSQAMRLRAAAIHAELGDRAQ
                     AIALYRGVMEEQPDDLATVRTLGDLLEAEGRHPELLTLRHHELSRTQDPQRKLELRLD
                     IANIVGIVEQRGGRIEALRRNLEEQPSHDPSLAALTEVLAAKASPEALADFLGEHAER
                     REGIGDRPRAVRLWAQLATLAEQRLGDVDRAISSYRRVVTLEPTIDALDALARQHNAR
                     GEHEEAVPWLSRRLEMTTGPARTPIVMLLAEALLGADRRERAINVLARAVADQPQEAP
                     PRELLLRLYREAEAWGPLTGILQVSATQVTDRAKILEYLYEAAAIFRERLGTLNGAIP
                     VLAQLQELVPDDRGVLRSLGDALWTAGRYAEARVIFEQLLAEFGRRRSPDRAAVHTKL
                     AALARSEGNRIEALAQLELATNMDMANIPALQMLAELAEEEGDMARAERSYRALLLAA
                     RRQTTTQGDALGVAEALYELGRLARQRDQAGQASEMLEAATQTALQDDREAAKLQRTL
                     RKHGDTTLLIRVLRSRLANGPEGELRAQILAELGDALDATDQPRDALELRLQALQDAP
                     TNAELHRSVAERAAKLGQSRSYVDTMLTMVDRIRRKQEAGLAADLLIRSATITERELG
                     DLKRAEELYGRAEALGNRVVEAWMGMARIAAARGDNARQIELLERISTSADDEMTPEI
                     RSQAVFGLAQIRLAHADTRDAGVAGMRRALEQDPQYAVAEPILREGLRLAPDHADLLA
                     LYEQTVRAIGDRPALLDFLERRAMADDADPAIAHEAAQLAIDLANETGEGTERAEALL
                     ARTLDLARGLDDGHAIWALLTLTDRRKQAGDLAGAIAHLRDASAIAEPARVFGVGLEL
                     AGLAAASDDQRSLAADLYEELLTQDPANKAVWAPLMAVYKRLDDLQRLQDLVEATLQT
                     LGDTGERNALRLDLVQSILATTGRETDAVRILKDVLMEEPSHREAERLLAEVFERTGF
                     DTELVELLNQQIMTAQANNDLEGVVASALRLGDLHRRTQPDEAKHVYRAALDFAPTER
                     RLIEALLAQLDPESERRERAEVTERLLAIETGEAAVQRTLELARQWDQIGDREAVARV
                     LERGYKAAPESEELRARLETWYRDREDWPALAEFLVTSAGRASEPAVAVKLLREASEL
                     YADQLFDAARSAEVLRKAVVVDPVDMGLLRELVTRLADTGEHQSAIDELTQAIEWQPL
                     DQTTLVEFLRRRAEFGMIVGAEAQAAADLERAHELVGAELIPDLVDGLERWRSAANRR
                     NDRTGERAATLRLVEILGKEGAGEQARHALAEWVAREPGDAQALRMLVDMDAGEQRWE
                     AVIDGCTRLIAAETGPAQIEAVTRLVSACEQLGRPLDARAGLEHAYRDQPGNDAVRGR
                     LKQIYEGEENYPALARILIGEAGTLEDSGARFIMLRQAGELLLDEDPAAAADALRQSL
                     AIRPTDQTVILLLVDACNAAGHPEQASAVLDGAIEAMRGRRSPELCVLQHRKAAAAAA
                     AGDQAQQLHWLKEAHNTDRNNGDVAVELADLAEQLEDYDLAIRVLRSIALMEAAPLSR
                     AIAYLRQGYIAERRGDRQKAVLWSRKALMEDPNCTEATDFLRQLGEL"
     gene            complement(8236..>9920)
                     /locus_tag="VGB85_27505"
     CDS             complement(8236..>9920)
                     /locus_tag="VGB85_27505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137875.1"
                     /translation="EPAAQQTVLDWARELATAGIPIVVTSRDAELPELEALCEVASGD
                     SPPLRRVHVLPLARPPIDRFLASWARLDGAFSRVAPDRLKAEMAARPALSQLARRPAE
                     LAILTVLSAGTNRLPETLAELHGALLGALDLGQPAHRQLLARVVADPDSALDMPTRRA
                     WLAALVAVSVACDHVDLGEALGRDVRLGPTGSALLLAATRAWAEGGCPLRPVLADSPV
                     RVPRARCSDGHDDEMARLIWLGHHAGWWGPEAIFVLRRAVPARERLVDQRPSALRPRP
                     LLLALAALDAAWGPCVAPLLRALPLGLLLAEGGTLGPVTASVLYTDNPPNELRPVREL
                     FHRFLELELALVSLAADDTTLQAIVMEMAASRDLDLDGALDLVLAQEGVGPRRPRTPG
                     AREDPSERGAYLDLALNLAARLTPCGAPALVADLERARDAAIHYSDTFTGVLRTRLEQ
                     LAGFLIVLLVYAIDRRLLQPVRSTLRRSEVLAMQQMLGAPERVAAGFAGERRAEVTED
                     WRWVMRQPWAPHRIAGHLLARMPETLELGPDVVLPRCHAWLRAWLAQADVAA"
BASE COUNT         1178 a         3483 c         3914 g         1345 t
ORIGIN      
        1 cgtgtatacg gcggcaaaat ccgcggttcg gtggcaggcg ggatacgagc tgtttatccc
       61 ggcttcggtg accggcgcgt cgcaagcatc gcaggcagcg ccggctcaca acgccgacga
      121 tcgccagaaa cccgtgagaa ccgccgatat tgggcaaatt aggaaagccc acggagcgcg
      181 ctccgtgggc tttttcaaaa gatcacgaag cgctcacagc tcgccgagct ggcgcaggaa
      241 gtcggtcgcc tccgtgcaat tcgggtcctc catgagcgcc ttgcggctcc acagcaccgc
      301 cttttgccgg tcgccgcggc gctcggcgat gtaaccctgg cgcaggtagg cgatcgcgcg
      361 gctcagcggc gcggcctcca tcagggcgat gctccgcagc acgcggatcg cgaggtcgta
      421 gtcctcgagc tgctcggcga ggtccgccag ctcgacggcg acgtcgccgt tgttgcggtc
      481 ggtgttgtgc gcctccttga gccagtgcag ctgctgcgcc tggtcaccgg ccgccgcggc
      541 cgcggccgcc ttgcggtgct gcagcacgca cagctcgggc gagcgacggc cacgcatcgc
      601 ttcgatcgcg ccgtcgagca ctgcgctcgc ctgctccggg tggccggcgg cgttgcacgc
      661 gtcgaccaac agcaagatca cggtctggtc ggtgggccga atcgcgagcg actggcgcag
      721 cgcgtcggcg gccgccgccg ggtcctcgtc gagcagcagc tcgccggcct ggcgcagcat
      781 gatgaagcgc gcgccgctgt cctcgagcgt gcccgcctcg ccgatgagga tccgcgcgag
      841 cgcggggtag ttctcctcgc cctcgtagat ctgcttgagg cgcccgcgca cggcgtcgtt
      901 gccgggctgg tcgcggtacg cgtgctcgag gccggcgcgc gcgtccagcg ggcggccgag
      961 ctgctcgcac gcggacacca gccgcgtcac cgcttcgatc tgggccggtc cggtctcggc
     1021 ggcgatcagc cgcgtgcagc cgtcgatcac cgcctcccag cgctgctcgc cggcgtccat
     1081 gtcgacgagc atgcgcagcg cctgggcgtc gccgggctcg cgcgcgaccc actcggcgag
     1141 cgcgtgccgg gcctgctcgc cggcgccctc tttgccgagg atctcgacga ggcgcaaggt
     1201 ggccgcgcgc tcgccggtgc ggtcgttgcg gcggttggcc gcgctgcgcc agcgctcgag
     1261 gccgtcgacg aggtcgggga tcagctcggc gccgacgagc tcgtgcgcgc gctcgaggtc
     1321 cgccgcggcc tgggcctcgg cgccgacgat catgccgaac tcggcgcgcc ggcgcaggaa
     1381 ctcgaccagc gtcgtctgat cgagcggctg ccactcgatc gcctgcgtca gctcgtcgat
     1441 cgccgactga tgctcgccgg tgtccgcgag gcgcgtcacc agctcgcgca gcaggcccat
     1501 gtcgaccggg tcgaccacga ccgccttgcg cagcacctcg gccgagcgcg ccgcgtcgaa
     1561 cagctggtcg gcgtacagct cggaggcctc acgcagcagc ttcaccgcga ccgccggctc
     1621 gctcgcgcga ccggccgaag tgaccaggaa ctcggccagc gccggccagt cctcgcggtc
     1681 gcggtaccat gtctcgagcc gcgcgcgcag ctcctcgctc tcgggggcgg ccttgtagcc
     1741 gcgctcgagc acgcgggcga ccgcctcgcg gtcaccgatc tggtcccact ggcgtgcgag
     1801 ctcgagggtc cgctgcaccg cggcctcgcc ggtctcgatc gccagcaggc gctcggtgac
     1861 ctcggcgcgc tcgcgtcgtt cgctctcggg gtcgagctgg gccagcaagg cctcgatcag
     1921 gcgccgctcg gtcggcgcga agtccagcgc ggcgcggtac acgtgcttgg cctcgtccgg
     1981 ctgcgtgcgg cggtgcaggt cgcccaggcg cagcgcgctg gcgaccacgc cctcgaggtc
     2041 gttgttcgcc tgcgccgtca tgatctgctg attgagcagc tcgacgagct cggtgtcgaa
     2101 gccggtgcgc tcgaacacct ccgcgagcag gcgctcggcc tcgcggtgcg agggctcctc
     2161 catcagcacg tccttgagga tgcgcaccgc gtcggtctcg cggccggtgg tggcgaggat
     2221 gctctgcacg aggtcgaggc gcagcgcgtt gcgctcgccg gtgtcaccga gggtctgcaa
     2281 ggtcgcctcg acgaggtctt gcaggcgctg gaggtcgtcg aggcgcttgt agacggccat
     2341 caggggcgcc cacacggcct tgttggcggg gtcctgggtg agcagctcct cgtagaggtc
     2401 ggcggcgagc gagcgctggt cgtcgctcgc ggcggcgagg ccggcgagct cgaggccgac
     2461 gccgaacacc cgcgccggct cggcgatcgc cgaagcgtcg cgcaggtggg cgatcgcgcc
     2521 ggcgaggtca ccggcctgct tgcgccggtc ggtgagggtc agcagggccc agatcgcgtg
     2581 cccgtcgtcg aggccgcggg cgagatcgag ggtccgggcg agcaacgcct cggcccgctc
     2641 ggtgccctcg cctgtctcat tggccaggtc gatcgccagc tgggcggcct cgtgggcgat
     2701 cgccgggtcg gcgtcgtcgg ccatcgcgcg gcgctcgagg aagtcgagca gcgccggccg
     2761 gtcgccgatc gcacgcacgg tctgctcgta caacgcgagc aggtcggcgt ggtcgggcgc
     2821 gaggcgcagg ccctcgcgca ggatcggctc ggcgaccgcg tattgcgggt cctgctcgag
     2881 cgcgcggcgc atgccggcga ccccggcgtc acgcgtgtcg gcgtgggcga ggcggatctg
     2941 ggcgaggccg aacaccgcct gcgagcggat ctccggcgtc atctcgtcgt cggccgaggt
     3001 cgagatgcgc tcgagcagct cgatctgccg cgcattatca ccgcgcgcgg cggcgatgcg
     3061 ggccatgccc atccacgcct cgacgacgcg gttgccgagc gcctcggcgc ggccgtacag
     3121 ctcctcggcg cgcttgaggt cgccgagctc gcgctcggtg atcgtcgcgc tgcggatcag
     3181 caggtcggcg gcgaggccgg cctcctgctt gcgccggatg cggtcgacca tcgtcagcat
     3241 cgtgtcgacg tagctgcggc tctggccgag cttggccgcc cgctcggcga cgctgcgatg
     3301 cagctcggcg ttggtcggcg cgtcctgcag cgcctgcagg cgcagctcga gggcgtcgcg
     3361 cggctggtcg gtcgcgtcga gcgcgtcgcc cagctcggcg aggatctgcg cccgcagctc
     3421 gccctcgggg ccgttggcca ggcgcgagcg cagcacgcgg atcagcaggg tcgtgtcgcc
     3481 gtgcttacgc agcgtgcgct gcagcttggc cgcctcgcgg tcgtcctgca gcgcggtctg
     3541 ggtcgcggcc tcgagcatct cgctcgcctg accggcctgg tcgcgctgac gggcgaggcg
     3601 accgagctcg tacagcgcct cggcgacgcc gagcgcgtcg ccctgcgtcg tcgtctgccg
     3661 gcgggccgcc agcagcagcg cgcgatacga gcgctcggcg cgggccatgt cgccctcttc
     3721 ctcggcgagc tcggcgagca tctgcagggc cgggatgttg gccatgtcca tgttggtggc
     3781 gagctcgagc tgggcgagcg cctcgatgcg gttgccctcg gagcgggcga gcgcggcgag
     3841 cttggtgtgc acggcggcgc ggtcgggcga gcggcggcgg ccgaactcgg cgagcagctg
     3901 ctcgaagatc acgcgggcct cggcgtagcg gccggcggtc cacagcgcgt cgccgaggct
     3961 gcgcagcacc ccgcgatcgt cgggcacgag ctcttgcagc tgcgcgagca cggggatcgc
     4021 accgttgagg gtgccgaggc gttcgcggaa gatcgccgcg gcctcgtaga ggtactcgag
     4081 gatcttggcg cggtcggtga cctgcgtggc cgacacctgc aagatgccgg tcagcgggcc
     4141 ccaggcctcg gcctcgcggt agagcctcag cagcagctcg cgcggcgggg cctcctgcgg
     4201 ctgatcggcg acggcgcggg cgagcacgtt gatcgcgcgc tcgcggcggt cggcgccgag
     4261 cagcgcctcg gcgagcagca tcacgatcgg cgtgcgcgcg gggcccgtcg tcatctcgag
     4321 gcgacggctg agccacggca cggcctcctc gtgctcgccg cgggcattgt gctggcgcgc
     4381 gagcgcgtcg agggcgtcga tcgtcggctc gagggtgacg acccggcgat aactcgagat
     4441 cgcgcggtcg acgtcgccga ggcgctgctc ggccagagtg gcaagctggg cccacagacg
     4501 cacggcgcgc ggcctgtcgc cgatgccctc gcggcgctcg gcgtgctcgc cgaggaagtc
     4561 ggcgagcgcc tccgggctgg ccttggcggc gagcacctcg gtcagcgccg cgagcgaggg
     4621 gtcgtgactc ggctgctcct cgaggttgcg ccgcagggcc tcgatccggc cgccgcgctg
     4681 ctcgacgatg ccgacgatgt tggcgatgtc gaggcgcagc tcgagcttgc gctgcgggtc
     4741 ctgcgtgcgt gaaagctcgt ggtggcgcag ggtcagcagc tcggggtgcc ggccctcggc
     4801 ctcgagcagg tcgccgaggg tgcgcacggt ggcgaggtcg tcgggctgct cctccatcac
     4861 gccgcggtac agcgcgatcg cctgggcgcg gtcgccgagc tcggcgtgga tcgcagcggc
     4921 gcgcaggcgc atcgcctgcg aggtcgcggg cgcgaacggc aggctcgcgc tgcggccgag
     4981 cagctcggcg gcctcgcggc gctcacccgc ctgatcgtgc aggcggccga gctgctgcac
     5041 ggcccacgcg acgctctcct ccggggtgcg cttgcccttg gccggctggc cgcggttcca
     5101 cagcgcggac gcgcgggtct gcagtcggcg caggatgtcg cgggtgcgcg gggtgtcgcc
     5161 gtcgaggccg agcgcgagct cggaggcctc gtagtgctcg tcgaagtcgt cctcgcgctg
     5221 gccggcgagc gcgagcaagg tgtcgatcag ctgggtgtcg ggtgccgcgc gttgcagctg
     5281 cgcgagcatc tcgaggcgac cgacgtcgtc gggcacgatc gccagcgagc gctgcagggc
     5341 cgagcgcgcc gcggcggcgt cgtcgcgatg atcgcggtgc cacagggcga cgcgcgcctc
     5401 aaggaacgcc gcgggcgtcg ccggcaggcg ggcctcggcg agcgcggcct cgagggcgag
     5461 ggtcaggccc tgccagccgc cgaactgcgc cgcctcggac tcgacgagcg tcagcagcga
     5521 ggcgtcgagc ttgccgtcgg cgccgacctg ggcgacgagg gccgcggccg cggcgtcccc
     5581 gcgggtgacg cgggccgcca tccgcaccgc gccgcgcagc gcgtcggcgt cacccggctc
     5641 gatcgccagg accgcgttgt aagcggcgag cgcggggtcg tgctcgccga gcttgtgctc
     5701 gagggtccgg gcctcggcga gccgcagctc ggcgctgcga cgcgggtcgc cgaccagcgt
     5761 cgccaccgcc tcgcggtagg cgtcgacggc gagctgccag gcgccggtct gctcggcgag
     5821 gcggacgatg tcccgctcga gctcgcggtc ccgcggggtg cgggggaagg cgcggcgcag
     5881 cagctccagg gccgcgcgcg gatcgccgac gtgctgttct tgcagggtcg ccgtctcgcg
     5941 caggatctcc gcctgcttgc gcgggtcgtc ggtgatcgcg aggcgagcct cgacgatgcc
     6001 ggcgagctcg ggccactcct cgcgcttgcg gtgcagcttg ccgagcagct cgaccgcacc
     6061 ggcgcgcgcg gccgggtcct cgagcgcggc gacgagaccc gcgcgcgcac cggcgtggcc
     6121 cgggtcgacc gagagcgccg cgtcgtagca ggcgaccgcg cgcgcggtct cgccgagctg
     6181 ggtgcggtag acgtcgccga ggcgggtcga gaggtcagcg acctgcgagg tctcgtgtcc
     6241 ggcggcgcgg gtcagcagct cggcgagcgc ctgccagcgg ccgacgcggg cgagcagcgc
     6301 ggccatcgcc tcgacggtct cgcggtcctc gccgaactct tgctgcacgc tctcccaggt
     6361 cgagatcgca gcggcggggt cgtcgagctg ggcggcctcg agggcggcga cgcgggtgag
     6421 gtcggcgcgg cgctggtgcg gcgcgaccgg gccgccggcg cgctggcgca gcgtctcgac
     6481 gagctcggcc cagcgaccga gctgctcgaa caggcggatc gcgtggcgca gcgcctcggg
     6541 gtcctcgccg tcgagggcga ggcgggcgcg ccagacctcg agcgcgcgct cgggctcgcc
     6601 gagctgctcg gccaggcgcg cggcgtcgcc gagcgactgg gcctgggcgg cgccgtcctc
     6661 gagcacgccg agccgctcga gcacggtcaa tctttcagcg tcgcggcccg cctccgcgag
     6721 caggtcctcg aggatcctcg ccacgcgcag ggcggtcgct ggcggggcgg cggcctccgc
     6781 gagcacgcgg gtgtacagct cgatcgcggc ggcgtggtcg tggaggatgt cgtgctggac
     6841 gtccgcggcc tcggcgagca gggcgacgcg gcgctgcacg tcggtggtcg cgtcggcggc
     6901 ggcgcgcagg ccctgcacgt gcacggcggc gtcgccggtg gtctcggcga ggtgacgcag
     6961 gcggcgctga tcttcgagcg agtcgggcgc gagggccagc aacgcgacgt ggtgctgcat
     7021 cgccggcacc ggccggtcga tcgccaccag gcgctcggcg atctcgcggt gcaggtcgat
     7081 cgcggtcgcc ggctcggagc ccggcaacgc gacctcgagc acgcggatca cctcttccgg
     7141 tcggtgcgcg tgctcgtagg tgctgcgcag cagatcgagc gcgccggcgc ggacgctggc
     7201 gtcggcgccc tcgagcacca gcaggcgctc gagcagcgcc gcggcgccgc ggtcgttcgc
     7261 gtcggtctgc aacagcgcgc gcgcctcggt cagcgcggcg gccgggtcgt gcaggcggtc
     7321 gagcgaggcc tcggcgatgc gggcctgcag gcccgggttg gcggccagac cttgcagctg
     7381 caagcgacgc ttccacagcg cgacgaggtc gcgccagcgc tcctgacgct cgagcaagcg
     7441 ctcgaggctg gcctcgaggc gctcgtcgcc ggggcgcagg gtcagcagct gatgcatgta
     7501 gccgacggcg cggtcggcct ggctggcgaa gtcgcgggcg atgtcggcgg cctcttccag
     7561 caattgcagg cggcgggtct cggcgacggg cgcggcgagg gtgcggtcat agagatcgag
     7621 cagctcggac cagcgctcgc gcagggtgta gacggacgtc agcttggcga acgcccactc
     7681 agcctgcggc tgggcggcga ggacccgcag cagcacatcg gtgaggctgg cgttgtcgtc
     7741 tccgagccag gtctcgtgga agtccgcggc acgtcggccg agctcgtcga cgacgtcggg
     7801 cgggagcttt tgtgtgaggg cctcgcggta ggccgcggcg acctcgtcga cctgttgatg
     7861 ggcctcaccg agctgctcgg cgaggtccca gtgctcgtgc ttgctgggtt cgctgcgcgc
     7921 tgcggcgagg gcggcggcga gggcttggtc gtcggtcggg tgggttgcca tcggatgcct
     7981 cggtcggggc tcgaggcggt ggctagctcg tggaccagcc ggcggcgcct cgacgaagag
     8041 tggggtcgcg agccctccgg gagcgagggc gacgatggca ccataccagg ctttcgccat
     8101 gcttttgcgt cagcgcgagg cgacgagcgc gggtgcgcgg ccgcgccgtc ggtcgttctt
     8161 tcccaggcgg ggcggcctca ttgcgaacga cgcatataca gcgatcggcg tggcggatcc
     8221 gcgcgggggg atctgtcagg cggcgacgtc ggcctgggcg agccacgctc gcagccacgc
     8281 gtggcagcgc ggcagcacga cgtcggggcc gagctcgagc gtctccggca tgcgcgcgag
     8341 caggtggccg gcgatgcggt gcggcgccca cggctggcgc atgacccacc gccagtcctc
     8401 ggtgacctcc gcccggcgct cgcccgcgaa gccggcggcg acccgctccg gcgcgccgag
     8461 catctgctgc atggcgagca cctcggagcg gcgcagggtc gagcgcaccg gctgcagcag
     8521 ccggcgatcg atcgcgtaga cgagcagcac gatcaaaaaa ccggcgagct gctcgagccg
     8581 ggtccgcagc accccggtga acgtgtcgct gtagtggatc gcagcgtcgc gggcgcgctc
     8641 gaggtcggcg acgagggccg gcgcgccgca cggggtcagg cgcgcggcga ggttgagcgc
     8701 gaggtcgagg taggcgccgc gctcgctcgg gtcctcgcgc gcgccggggg tccgcggacg
     8761 ccggggaccg acgccctcct gcgcgagcac gaggtcgagc gcgccgtcga ggtcgaggtc
     8821 gcgcgacgcc gccatctcca tgacgatcgc ctgcaaggtg gtgtcgtcgg cggcgaggct
     8881 gacgagcgcg agctccagct cgaggaagcg gtggaacagc tcgcgcacgg ggcggagctc
     8941 gttcggcggg ttgtcagtgt acagcaccga ggcggtgacc ggcccgagcg tgccaccctc
     9001 ggcgagcagc agcccgagcg gcagggcgcg cagcagcggg gcgacgcacg ggccccaggc
     9061 ggcgtcgagg gccgcgagcg cgagcagcag cggccgcggc cgcagggccg aggggcgctg
     9121 gtcgacgagg cgctcgcgcg ccggcacggc ccggcgcagc acgaagatcg cctcggggcc
     9181 ccaccagccg gcgtgatgac cgagccagat caggcgcgcc atctcgtcgt cgtggccgtc
     9241 gctgcagcgc gcccggggca cgcgcacggg gctgtccgcg aggacaggtc gcaagggaca
     9301 gccgccctcg gcccacgcgc gcgtcgccgc gagcaacagg gcgctgccgg tcgggccgag
     9361 ccgcacgtcg cggccgagcg cctcgccgag gtcgacgtga tcgcaggcga ccgagaccgc
     9421 gacgagggcc gcgagccagg cgcggcgggt cggcatgtcg agcgcgctgt cggggtcggc
     9481 gaccacgcgc gcgagcagct gacggtgcgc gggctggccg aggtcgagcg cgccgagcag
     9541 cgcaccatgc agctcggcga gggtctccgg cagcctgttc gtgccggccg acagcacggt
     9601 gaggatcgcc agctcggccg ggcgtcgcgc gagctggctg agcgccggcc gggcggccat
     9661 ctcggccttg aggcggtccg gcgcgacgcg gctgaaggcg ccgtcgaggc gcgcccagct
     9721 ggcgaggaag cggtcgatcg gcgggcgcgc gagcggcagc acgtgcacgc ggcgcagcgg
     9781 gggcgagtcg ccactcgcca cctcgcacag cgcctcgagc tccggcagct cggcgtcgcg
     9841 gctggtgacg acgatcggga tccccgcggt cgcgagctcg cgggcccagt cgagcacggt
     9901 ctgctgcgcc gccggctcgc
//
LOCUS       DASWNJ010001657         1725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_451593, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001657 DASWNJ010000000
VERSION     DASWNJ010001657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451593"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..>1725)
                     /gene="ppk1"
                     /locus_tag="VGB85_27510"
     CDS             complement(169..>1725)
                     /gene="ppk1"
                     /locus_tag="VGB85_27510"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003918.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEY0137876.1"
                     /translation="LPPEVAEVANGFALLGSIVTTFVRKLFPGMNVTGAYQFRVTRDS
                     ELWVQEEDVEDLLNALKGELRTRNFGDAVRLEVSETCPQEMSDFLLQKFGLHQDDLYR
                     VDGPVNLHRLATLYDVIKRPELKFPAFIPGPKRVRGADASDFFVDLRRGDILLHHPFE
                     SFSPVLEMVRQAAADPSVLAIKQTLYRTGPASPMAEALLEAARAGKEVTAVVELRARF
                     DEAANIDLATRLQQAGVTVVYGIVGYKTHAKMLLIVRRDEAGRLRRYVHLGTGNYHTV
                     TTQFYSDFSFLTSNEQIGEDVHNVFMQLTGFGQGKPLHKLLMSPFTLMDRLLAMVDRE
                     IEHARAGKPAHIICKVNGVTETRLIEAFYRASQAGVSIDLIVRGICCIRPGVPGVSEN
                     IRVRSIVGRFLEHHRVYYFRNDGQEQVYCGSADLMSRNLVRRVEIGFPIEDPVLRARV
                     IQEGLMTYLADNVQAWELQPDGSYVRCQPGEEVRSAQESLMRQLSASTVAAHTQLLLE
                     DILAREDLLE"
BASE COUNT          284 a          556 c          614 g          271 t
ORIGIN      
        1 ccgggccatt gcgacgacac gccgaccccc gagacgaccg gcggcgagac gaccggcggc
       61 gacactggtg gcgacacgac cggtggcgag accggaaccg actgcatcgt cgactgcggc
      121 accacgaccg gctccacgac cgaccccatc cccggctgag ctggtgcgct actcgaggag
      181 gtcctcgcgg gcgaggatgt cctcgagcag cagctgcgtg tgtgcggcga cggtgctcgc
      241 cgacagctgg cgcatcagcg attcctgggc ggagcggacc tcctcgcccg gctggcagcg
      301 cacgtagctg ccgtcgggct ggagctccca cgcctggacg ttgtcggcga ggtaggtcat
      361 caggccctcc tggatcacgc gggcgcgcag gaccgggtct tcgatcggga agccgatctc
      421 gacgcggcgc accaggttgc gcgacatcag gtcggcggag ccgcagtaga cctgctcctg
      481 gccgtcgtta cgaaaatagt acacgcggtg gtgctcgagg aagcggccga cgatcgaccg
      541 cacgcggatg ttctccgaca cgccgggcac gcccgggcgg atgcagcaga tgccgcgcac
      601 gatcaggtcg atgctgaccc ccgcctgcga ggcccgataa aacgcctcga taagccgcgt
      661 ctcggtgacg ccgttgacct tgcagatgat gtgggccggc ttgccggcgc gcgcgtgctc
      721 gatctcgcgg tcgaccatcg ccagcagccg gtccatcagc gtaaacggcg acatcagcag
      781 tttatgcaat ggcttgcctt gaccgaagcc ggtcagctgc atgaacacat tgtgcacatc
      841 ctcgccgatc tgctcgttgc tggtgaggaa gctgaagtcg ctgtagaact gcgtcgtgac
      901 ggtgtgatag ttgccggtgc cgaggtgcac gtaacggcgc aggcggccag cttcgtcgcg
      961 gcggacgatc aacagcatct tggcgtgcgt cttgtacccg acgatgccgt acacgacggt
     1021 gacgccggcc tgctgcaggc gcgtggcgag gtcgatgttg gcggcctcgt cgaagcgcgc
     1081 gcgaagttcg acgaccgcgg tgacctcctt gccggcgcgc gccgcctcga gcagggcctc
     1141 ggccatcggc gaggccgggc cggtgcggta cagcgtctgc ttgatcgcca gcaccgacgg
     1201 gtcggccgcg gcctggcgga ccatctcgag caccggtgag aagctctcga aggggtggtg
     1261 cagcaagatg tcgccgcggc gcaggtcgac gaagaagtcg ctcgcgtcgg ccccgcgcac
     1321 gcgcttgggg ccggggatga aggccgggaa cttgagctcg ggccgcttga tcacgtcata
     1381 aagcgtggcg aggcggtgca ggttgacggg gccgtcgacg cggtagaggt cgtcctgatg
     1441 cagcccgaat ttttgtagaa ggaagtccga catctcctgc gggcaggtct ctgaaacctc
     1501 gaggcggacc gcgtcgccga agttgcgggt ccgcagctcg cccttgaggg cgttgagcag
     1561 gtcctcgacg tcctcctcct gcacccacag ctcggagtcg cgggtgacgc ggaactggta
     1621 cgcgccggtg acgttcatgc ccgggaacag cttgcgcacg aaggtcgtga cgatcgaccc
     1681 gagcagcgcg aagccgttgg cgacctcggc gacctccggc ggcag
//
LOCUS       DASWNJ010001658         2132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_595240, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001658 DASWNJ010000000
VERSION     DASWNJ010001658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595240"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1364
                     /locus_tag="VGB85_27515"
     CDS             <1..1364
                     /locus_tag="VGB85_27515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137877.1"
                     /translation="PTRPMPIVPPEGPPARRSITPAPHPTPVQEPPPPLRRASTPAPI
                     DDLPPPVRRTPAPADDDLPPPVRRTTTPAPSDDLPPPVRRTGGMDDTLRPARPSWPPA
                     DAVEGDGPPVVARARTAPVPRAEPVPAPATEAADLEQAWADPTVRRIIITGLQRVEVE
                     RDGVCLPLARGFSSVDDLGDRLAALCGRPILRESPTECVLASGTAVQAILDGDRGPHV
                     VITRARHKRGGGLDALVQDRVLPALAAELLVGCLRAKLPLLVVAGPGVGALPLISGLL
                     GALEGSSGLVRGLPAPAVTPRGCVAIDAAHGSGHMAAAVRLVLATEVSTLAVDEPEPA
                     ALEECAWASVGGRGVLVGVRAASLEQALARAHGVATTSTGPDIAYSLAIVLGVPRGAP
                     VGSFTVTRVVELRRDDQGPGSLQEIFGVGRRGELIPIAVPAVLAELTQRGVHIAPELF
                     TPM"
     gene            1501..>2132
                     /gene="tssJ"
                     /locus_tag="VGB85_27520"
     CDS             1501..>2132
                     /gene="tssJ"
                     /locus_tag="VGB85_27520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HEY0137878.1"
                     /translation="MCTASSLVVLAALVSSAGCKPKASCQPGEYTVQVVLHPGEPLNP
                     GDNQESLPTTVHVVQLADDEAVGRFDLEAFRADPQAAIGESYISHETFTVWQKTDDVR
                     KLRPKAETQHLMLLAEYRQVLGSGWYATYDVPLQKTHEDAVCTAVKRKKPPLPDPCFY
                     VLLERYETRGGAQPPAGMKGDQVRIRGKAVRCAPPPHQYDIDPKIAKKKNR"
BASE COUNT          281 a          877 c          668 g          306 t
ORIGIN      
        1 gcccgacgcg gccgatgccg atcgtgcctc ccgagggccc gcctgcgcgt cgcagcatca
       61 ccccggcccc gcacccgacc cccgtgcagg aaccgccgcc accgctgcgt cgcgccagca
      121 cgcccgcgcc gatcgacgac ctccctccgc ccgttcgtcg cacccccgcg cccgccgacg
      181 acgacctccc tccccccgtt cgtcgcacca ccacgcctgc gccctccgac gacctccccc
      241 cgcccgttcg tcgcaccggc ggcatggacg acacgctcag gcccgcgcgt ccctcctggc
      301 cgcccgccga cgccgtcgag ggtgacggcc cgccggtggt cgctcgcgcg cgcacggccc
      361 cggtcccgcg cgccgagccc gtgcccgcgc ccgcgaccga ggccgcggac ctcgagcagg
      421 cgtgggccga cccgaccgtc cgccgcatca tcatcaccgg gctacagcgc gtcgaggtcg
      481 aacgcgacgg cgtctgcctc ccgctcgcgc gcggcttctc ctccgtcgac gacctcggcg
      541 atcgcctcgc cgcgctgtgc ggccgaccga tcctccgcga gtcaccgacc gagtgcgtgc
      601 tcgccagcgg caccgcggtg caggcgatcc tcgacggaga tcgcggcccg cacgtcgtca
      661 tcacccgtgc gcgtcacaag cgtggcggcg gactggacgc gctcgtgcag gaccgggtcc
      721 tgccggccct cgccgccgag ctgctcgtcg gctgcctgcg cgccaagctc cccttgctcg
      781 tcgtcgccgg tcccggtgtc ggcgccctcc cgctcatctc cggcctgctc ggcgcgctcg
      841 agggatcgtc cgggctcgtg cgtggcctgc cggccccggc ggtcacaccg cggggatgcg
      901 tagcgatcga cgccgcccac ggcagcgggc acatggccgc cgccgtccgc ctcgtgctcg
      961 ccaccgaggt ctccacgctc gccgtcgacg agcccgagcc cgccgccctc gaagaatgtg
     1021 catgggcaag cgtcggcggc cgcggcgtgc tcgtcggcgt gcgcgcagcc tcgctcgaac
     1081 aagcgctcgc tcgcgcgcat ggggtcgcca ctacctccac aggcccagat atcgcctatt
     1141 cgctggccat cgtgctcggc gtcccccggg gcgcgccggt cggctcgttc acggtcacgc
     1201 gggtcgtcga gctgcgtcgc gacgaccagg gccccggcag cctgcaagag atcttcggcg
     1261 tcggtcgtcg tggtgagctc atcccgatcg ctgtccccgc cgtgctcgcc gagctgacgc
     1321 agcgtggcgt gcacatcgcg cccgaactat ttacgcccat gtgatcggcg tcatcgcgat
     1381 tgtgcttcgc gtgctcgcga ggcgctcgcc ttgcaatggt cgagccccgt cgatactctg
     1441 ggtcgctcga aagccatgat ggtcttttca cgcctatcgc gggtctgtgc atgcgcgcgc
     1501 atgtgcaccg cttcatccct cgtcgtcctg gccgccctcg tttcgtccgc cggctgcaag
     1561 ccgaaggcga gctgccagcc cggcgaatat accgtccagg tcgtgctcca ccccggtgag
     1621 ccgctcaacc ccggcgacaa ccaggagtcg ctgccgacca ccgtgcacgt cgtgcagctc
     1681 gccgacgacg aggccgtcgg tcgcttcgac ctcgaggcct ttcgcgccga cccacaggcc
     1741 gcgatcggtg agagctacat ctcccacgag accttcaccg tctggcaaaa gaccgacgac
     1801 gtccgcaagc tgcggcccaa ggccgagacc cagcatctca tgctgctcgc cgagtaccgc
     1861 caggtgctcg gcagcggctg gtacgccacc tacgacgtcc cgctgcaaaa gacccacgag
     1921 gacgcggtgt gcaccgccgt caagcgcaag aagcctcccc tcccggaccc ctgcttttac
     1981 gtcttgctcg agcgctacga gacccgcggc ggcgcccagc cgcccgccgg catgaagggt
     2041 gaccaggtcc ggatccgcgg caaggccgtg cgctgcgccc cgccgccgca ccagtacgac
     2101 atcgacccga agatcgccaa gaagaagaac cg
//
LOCUS       DASWNJ010001659         5276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_277373, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001659 DASWNJ010000000
VERSION     DASWNJ010001659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277373"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /gene="proB"
                     /locus_tag="VGB85_27525"
     CDS             complement(<1..804)
                     /gene="proB"
                     /locus_tag="VGB85_27525"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEY0137879.1"
                     /translation="MSPRQDIAAARRIVIKLGTHVITHPEAGLALGRLCSLVEGVAAV
                     NRQGREVVLVSSGAVGMGQRALGLRRRPDSLGLRQACAAVGQSQLMALYSQNFGQLGV
                     KVAQVLLTQEDLGDRDRALCVRTTLTRLLELDVVPILNENDSVSVRELVEYRRGQGVE
                     GPTLEFGDNDGLSARVAVALDADLLVLLTDVDGLFTANPRTDPTACRIAELADIDETT
                     LARATGSSTGGTGGMASKLEAARLASRAGIPVLITSGHHPGALELALGGA"
     gene            complement(839..1582)
                     /locus_tag="VGB85_27530"
     CDS             complement(839..1582)
                     /locus_tag="VGB85_27530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137880.1"
                     /translation="MSSKPQPPGRPHLHVVKGGRDAAGVVEVDASFVGERTLGVVDAT
                     ARPAPKALTAGAAEPVPEDMSEEAGRRLAPGAPLSPGTVPLELALHLRSGEALVWWDA
                     KAAIDFRPILVVLGACALVLLGVTAMAPDFWAQPWSDLWPPVAALLSPALLLVLREQL
                     SLRSLMVTDTAIIEITRRGEVSRLVFSAIRTVRRDLLTGGVLLQGKGTRIRVPPALIE
                     NARAAIVTQRKSVLRGESTQPDDPLHWMP"
     gene            complement(1579..2688)
                     /gene="aroC"
                     /locus_tag="VGB85_27535"
     CDS             complement(1579..2688)
                     /gene="aroC"
                     /locus_tag="VGB85_27535"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY0137881.1"
                     /translation="MSNSFGTTFRVTTFGESHGGGLGVVVDGCPAGHPFPFARLAAQM
                     ARRKPGQGKLTSPRQEADEVRVLSGLEPDTNLTLGTPIAMLVGNSDARSHHYDDLAAL
                     YRPSHADYTYDAKYGVRAVAGGGRASARETVGRVAAGALAEDLLGTLHGVEIVAWVAE
                     VAEVVAPIPDLAALTRDQVDASPVRCPDPAAAEAMIAAIDGARRDGDTVGGVIRCVAR
                     NVPAGWGAPVFDKLTAALAQAMMSLPASRGFEVGDGFASTRLRGSQHNDPFAIDETTG
                     QIHTTSNHSGGIQGGISNGEMLRLAVAFKPVATIFRPQHTVTRDLQPVVFQPKGRHDP
                     CVLPRAVPMVEAMAALVLCDHMLRARLARVADLRP"
     gene            complement(2681..3535)
                     /locus_tag="VGB85_27540"
     CDS             complement(2681..3535)
                     /locus_tag="VGB85_27540"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0137882.1"
                     /translation="MPELITIPDRVAGPGVLGRVRVDRRAAQAGGEPSLVILGGMTQT
                     LASWGGQVRPLSAGREVLVYEARGQGQSELAITDVSPARHVEDFAALLAALAIPGPVD
                     LCGFSFGGRIALAIAATRPELVRRLIVTGVSAGRGALGRVIVRAWQASLATGNLEALA
                     WLSLADTLGPAYLDRNEHMLAGMVQATMSRNRYEGIRALFEQTLADDPAWEFHPTRLA
                     PRIACPTLLLAGAHDRLAPELDSLAAVFAGPVTHHIVPDVGHTVAIEAPEVWRAHVLE
                     FLAAPSHE"
     gene            3649..3864
                     /locus_tag="VGB85_27545"
     CDS             3649..3864
                     /locus_tag="VGB85_27545"
                     /inference="COORDINATES: protein motif:HMM:NF016028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HEY0137883.1"
                     /translation="MTDSQVPTREELERRIVDLEVRAAFQTQTIELLDGVVREFAARV
                     ESLERAMIEIRGQIDVPGELEADESEA"
     gene            3957..5147
                     /locus_tag="VGB85_27550"
     CDS             3957..5147
                     /locus_tag="VGB85_27550"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEY0137884.1"
                     /translation="MRPPVLRAWWAARQGLLALDGEASPAAVLGRSGWARSVGGVNPY
                     MTLFARAGTSRATAEEAAARRQIHELPGARGCTYIVPREDFALALRVGQGFSEAAELQ
                     MARKHLGVTDPEIDRLCEGVLAALGEGAKDPAALKMSLGDTVRNLGEAGKKRGMTTTL
                     PLALALLQSKGEILRQPVGGRLDQQRYAYTLWTDSPLRGDTRTVEEAQVELARKYFRW
                     IGPASAAHFQWFSGLPQKAVKAAIAPLGLVPLEAGSPLLMHPEDHEALVRFEPPGDPV
                     YRLVAGIDALFLLRRDMQDLLDPDDAARKIVGDRGAVELGGLQDLTSHAIVDRGRVIG
                     LWEYDPEASTIAWSSFVKPTPALRAEVERTQTFIREQLGDARAFSLDSPEKRRPRIDA
                     LRQA"
BASE COUNT          729 a         1770 c         2107 g          670 t
ORIGIN      
        1 ggcgccgccg agcgcgagct cgagggcgcc ggggtgatgg ccgctggtga tcaagacggg
       61 gatgccggcg cggctggcga ggcgcgccgc ctcgagcttg ctcgccatgc cgccggtgcc
      121 gccggtggag ctgccggtgg cgcgggcgag ggtggtctcg tcgatgtcgg cgagctcggc
      181 gatccggcac gcggtcgggt cggtgcgcgg gttggcggtg aagaggccgt cgacgtcggt
      241 cagcaggacc agcaggtcgg cgtcgagggc gacggcgacg cgcgcagaca ggccgtcgtt
      301 gtcgccgaac tcgagggtcg gaccctcgac gccctgaccg cggcgatact cgacgagctc
      361 gcgcacgctg acgctgtcgt tctcgttgag gatcgggacg acgtcgagct cgagcaggcg
      421 ggtcagcgtg gtgcgcacgc acagcgcacg gtcgcggtcg ccgaggtcct cctgcgtcag
      481 caacacctgc gcgaccttga cgccgagctg gccgaagttc tggctgtaga gcgccatcag
      541 ctggctctgg ccgacggcgg cgcaggcctg gcgcagcccg agcgagtcgg ggcggcggcg
      601 cagcccgagc gcgcgctgac ccatgccgac ggcgccgctc gagacgagca cgacctcgcg
      661 accctgacgg ttgacggcgg cgacgccctc gacgaggctg cacaggcgac cgagcgcgag
      721 gccggcctcg gggtgggtga tgacgtgcgt gccgagcttg atgacgatcc ggcgggcggc
      781 ggcgatgtcc tggcgggggc tcacatccgc gagtgtgcca cagcgcgcga gcgcgggctc
      841 agggcatcca gtgcagcggg tcgtcgggct gggtcgactc gccgcgcagc acgctctttc
      901 gctgggtgac gatcgcggcc ctggcgttct cgatcagcgc cggcggcacg cgaatgcggg
      961 tgcccttgcc ttgcagcagc acgccgccgg tcaggaggtc gcggcggacg gtgcggatcg
     1021 ccgagaacac caggcgcgag acctcgccgc ggcgcgtgat ctcgatgatc gcggtgtcgg
     1081 tgaccatcag cgagcgcagg ctcagctgct cgcgtaacac cagcaacagg gcgggcgaca
     1141 gcagggcggc gaccggcggc cacaggtccg accagggctg cgcccagaag tcgggcgcca
     1201 tcgcggtgac gccgagcagc accagcgcgc acgcgccgag cacgacgagg atcgggcgaa
     1261 agtcgatcgc ggccttggcg tcccaccaca ccagcgcctc gccgctgcgc aggtgcagcg
     1321 ccagctcgag cgggacggtg ccgggcgaca gcggggcgcc cggggcgagc cgacggccgg
     1381 cctcctcgga catgtcctcg gggacaggtt cggcggcgcc ggcggtgagg gccttcgggg
     1441 cggggcgggc ggtcgcatcg acgacgccga gagtgcgctc gccgacgaag ctggcgtcga
     1501 cctcgacgac gccggcggcg tcccgaccgc ccttgacgac gtgcaggtgc ggtcgacccg
     1561 gaggctgtgg tttgctgctc atggtcgcag gtccgccacg cgcgcgaggc gggcgcgcag
     1621 catgtggtca cacaggacca gcgcggccat cgcctcgacc atgggcacgg cgcggggcag
     1681 cacgcacggg tcgtggcggc ccttgggctg gaagacgacg ggctggaggt cgcgggtcac
     1741 ggtgtgctgc gggcgaaaga tggtggcgac gggcttgaag gcgacggcga ggcgcaacat
     1801 ctcgccgttg gagatcccgc cctggatgcc gccactatga ttgctcgtcg tgtggatctg
     1861 accggtcgtc tcgtcgatcg cgaacgggtc gttgtgctgc gagccgcgca ggcgcgtgct
     1921 ggcgaagccg tcgccgacct cgaagccgcg gctggccggc aggctcatca tcgcctgcgc
     1981 gagcgcggcc gtcagcttgt cgaacaccgg cgcgccccag ccggcgggga cgttgcgcgc
     2041 gacgcagcgg atcacgccgc cgacggtgtc accgtcgcgc cgggcgccgt cgatcgcggc
     2101 gatcatcgcc tcggcggcgg cggggtcggg gcagcgcacc gggctcgcgt cgacctggtc
     2161 gcgcgtcagc gcggcgaggt cggggatcgg cgcgacgacc tcggcgacct ccgcgaccca
     2221 ggcgacgatc tcgacgccgt gcagggtacc gagcaggtcc tcggcgagcg cgccggcggc
     2281 gacgcggccg acggtctcgc gcgcggaggc ccgaccgccc ccggcgaccg cacgcacgcc
     2341 gtacttggcg tcgtaggtgt agtcggcgtg cgagggccgg tacagcgcgg cgaggtcgtc
     2401 gtagtgatgg ctgcgcgcgt cgctgttgcc gacgagcatg gcgatcggtg tgccgagggt
     2461 gaggttggtg tcgggctcga ggccgctgag cacgcggacc tcgtcggcct cctggcgcgg
     2521 cgaggtcagc ttgccctgcc cgggcttgcg gcgggccatc tgcgcggcga gccgggcgaa
     2581 cgggaagggg tggcccgcgg ggcaaccatc gacgacgacg ccgaggccgc cgccgtgcga
     2641 ctcgccgaag gtggtcacgc gaaaggtggt gccgaaggag ttactcatga gagggggcag
     2701 cgaggaactc gaggacgtgc gcgcgccaga cctcgggcgc ctcgatcgcg acggtgtggc
     2761 cgacgtcggg cacgatgtgg tgggtgacgg ggccggcgaa cacggcggcg aggctgtcga
     2821 gctcgggcgc gaggcggtcg tgcgcaccgg cgagcagcag ggtcgggcag gcgatccgcg
     2881 gggcaaggcg ggtcgggtga aactcccacg ccgggtcgtc ggcgagggtc tgctcgaaca
     2941 gcgcgcggat gccctcgtag cggttgcgcg acatggtcgc ctggaccatg ccggcgagca
     3001 tgtgctcgtt gcggtcgagg taggccgggc cgagggtgtc ggcgaggctc agccacgcga
     3061 gcgcctcgag gttgccggtc gccagcgagg cctgccaggc gcgcacgatg acgcggccga
     3121 gcgcaccacg gcccgcgctg acgccggtga cgatcagacg acgcacgagc tcggggcggg
     3181 tcgcggcgat cgccagcgcg atccggccgc cgaacgagaa gccgcacagg tcgacggggc
     3241 cggggatcgc gagggccgcg agcaaggcgg cgaagtcctc gacgtgccgc gcgggcgaga
     3301 cgtcggtgat cgccagctcg ctctgaccct ggccacgcgc ctcgtagacg agcacctccc
     3361 gcccggccga caacgggcgc acctggccgc cccagctggc gagggtctgc gtcatgccgc
     3421 cgaggatcac gagggacggc tcgccgccgg cctgcgcggc gcgccggtcg acacggacgc
     3481 gcccgaggac gccaggaccg gcgacgcggt cggggatcgt gatcagctcg ggcatcgcgg
     3541 cgggtgagcc taccccggcc ccgccggctt gtctgccggc cccccgcggg gcggtgtttc
     3601 gcggcccagg gcttgccagg cgcgctgcgc ggcggcaaaa agccccgcat gaccgattcg
     3661 caagtaccca cccgcgagga gctcgagcgc cgcatcgtcg acctcgaggt ccgcgcggcc
     3721 ttccagacgc aaaccatcga gctgctcgac ggcgtggtgc gagagttcgc ggctcgcgtc
     3781 gagagcctcg agcgcgcgat gatcgagatc cgcgggcaga tcgacgtccc gggcgagctc
     3841 gaggccgacg agtccgaggc ctgagacccg ggctgacggg tcggggccgc ctgcaagcgc
     3901 agagggcggt gcccgcgggc gcgcgagatg gcgcgtttcc gggtatgctc gcgcggatgc
     3961 gacccccggt cttgcgtgcg tggtgggcgg cccgacaggg gctgctggcg ctcgatggcg
     4021 aggcgagccc ggccgcggtg ctcggccgca gcggctgggc gcgcagcgtc ggcggggtca
     4081 acccgtacat gacgctgttc gcccgcgccg gcacctcgcg cgcgaccgcg gaggaggccg
     4141 cggcacggcg ccagatccac gagctgccgg gcgcccgcgg gtgcacgtac atcgtgccgc
     4201 gcgaggactt cgcgctggcg ctgagggtcg gccagggctt tagcgaggcc gcggagctgc
     4261 agatggcgag gaagcacctc ggggtgaccg atccggagat cgatcggctg tgcgaggggg
     4321 tgctcgcggc gctgggcgag ggggccaagg atccggcggc gctcaagatg tcgctcggtg
     4381 acacggtgcg taacctcggc gaggccggca agaagcgcgg catgacgacg acgctgccgc
     4441 tggccctcgc gctgctgcag agcaagggcg agatcctgcg ccagccggtc ggcgggcgcc
     4501 tcgaccagca gcgttatgcg tataccctgt ggaccgactc gccgctgcgc ggcgatacgc
     4561 gcacggtcga ggaggcgcag gtcgagctgg cccgcaagta cttccgctgg atcgggccgg
     4621 cctcggcggc gcacttccag tggttctcgg ggctgccgca aaaggcggtc aaggccgcga
     4681 tcgcgccgct ggggctggtg ccgctggagg ccgggagccc gttgctgatg cacccggagg
     4741 accacgaggc gctggttcgc ttcgagccgc ccggtgatcc ggtgtaccgc ctggtcgccg
     4801 gcatcgacgc gctgttcctg ctgcgccgcg acatgcagga cctgctcgat ccggacgatg
     4861 cggcgaggaa gatcgtcggc gatcgcgggg cggtcgagct cgggggtctg caggacctga
     4921 cgagccacgc gatcgtcgat cgcggacggg tgatcggcct gtgggagtac gacccggagg
     4981 cgagcacgat cgcctggagc agcttcgtga agccgacgcc ggccctgcgc gccgaggtcg
     5041 agcgcacgca gacctttatc cgcgaacagc tcggcgacgc ccgcgcgttc agcctcgata
     5101 gccccgaaaa acgccgcccg aggatcgatg cgctgcgcca ggcatgaagc acggcacgct
     5161 gtgcgagcgg cggcgcggcg tggcagcgac tcgccgatgc ggatcctcgt ggggtcgatc
     5221 ctgctcgcgc tgacgccggc ctgctcgacg gcaaagcctc tgttcgagct cgccac
//
LOCUS       DASWNJ010001660         1542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_699319, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001660 DASWNJ010000000
VERSION     DASWNJ010001660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699319"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /gene="coaBC"
                     /locus_tag="VGB85_27555"
     CDS             complement(<1..732)
                     /gene="coaBC"
                     /locus_tag="VGB85_27555"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEY0137885.1"
                     /translation="MPEGPRRILLIVAGGIAAYKAPELVRALIAGGFTVQVVMTRAAG
                     AFVSELSLATVSTRAVRTSLLDPAEEGRVGHIELADWPDLVIVAPGTADLIARAAAGL
                     ADDLAATVLLATRAPVLWAPAMNTNMWRHPATRHNLATLAARGARFIGPDRGELACGW
                     VGEGRMIDPSAIVQEARRVLDDLSQGTGSWAGRRVLVSAGPTRSYIDPVRFFSNASTG
                     VMGFAIAAAAHQLGAEVTLVAGPVEL"
     gene            complement(725..>1542)
                     /locus_tag="VGB85_27560"
     CDS             complement(725..>1542)
                     /locus_tag="VGB85_27560"
                     /inference="COORDINATES: protein motif:HMM:NF013118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEY0137886.1"
                     /translation="TDAQFGAADIVSPGHIFPLRAQPGGVLVRSGHTEGSVDLARLAG
                     LRPAGVICEIMRDDGEMARMDDLERFAHKHDLPILTIADLIGYRLQRELLVEEFARTP
                     LVSKLLGVEAEHGWTVHSFRSKVSPHSFYIALCKGDLQQSASGDDSVLLRAQRAQIMG
                     DVFGLADDDSASRMRASIRQIERAGRGVFLYVLGHNWPPEPLLQSPAPANAPALKPAE
                     AGFREFGLGAQVLRSLGIHRIRILTNNPRKIVGLSGFGIETLGALPLEEDGGA"
BASE COUNT          210 a          513 c          622 g          197 t
ORIGIN      
        1 cagctcgacg ggccccgcga ccagcgtcac ctcggcgccg agctggtgcg cggccgcggc
       61 gatcgcaaag cccatcacgc cggtcgaggc gttggagaag aagcgcaccg gatcgatgta
      121 gctgcgcgtc ggaccggcgg acacgagcac gcggcggccg gcccacgagc ctgtcccctg
      181 ggacaggtcg tcgagcacgc ggcgtgcctc ctggacgatc gcggatgggt cgatcatgcg
      241 gccctcgccg acccagccgc aggcgagctc gccgcggtcg gggccgatga agcgggcgcc
      301 gcgggccgcg agcgtggcga ggttgtgccg ggtggccggg tggcgccaca tgttggtgtt
      361 catcgccggg gcccacagga ccggggcgcg ggtcgccagc agcacggtcg cggcgaggtc
      421 gtcggcgagg ccggccgcgg cgcgggcgat caggtcggcg gtgcccggcg cgacgatcac
      481 gaggtcgggc cagtcggcga gctcgatgtg ccccaccctg ccctcctcgg ccgggtcgag
      541 caggcttgtc cgcacggcgc gggtcgagac ggtggcgagc gagagctcgc tgacgaacgc
      601 gccggcggcc cgggtcatga cgacctggac cgtgaagccg ccggcgatca gggcgcggac
      661 cagctcgggc gccttgtagg cggcgatgcc cccggcgacg atcagcagga tccgtcgtgg
      721 gccttcaggc accaccgtcc tcctcgagcg ggagggcgcc gagggtctcg atgccgaagc
      781 ccgagaggcc gacgatcttg cgggggttgt tggtcaggat ccggatccgg tggatgccga
      841 ggctgcgcag gacctgggcg ccgaggccga actcgcgaaa gcccgcctcg gccggcttga
      901 gcgcgggggc gttggccggg gccggcgact gcagcagcgg ctcgggcggc cagttgtggc
      961 cgagcacgta caggaacacg ccccggccgg cgcgctcgat ctggcggatg ctcgcgcgca
     1021 tgcgcgaggc gctgtcgtcg tcggcgaggc cgaacacgtc gcccatgatc tgcgcccgct
     1081 gggcccgcag cagcacgctg tcgtcgccgc tggcggactg ctggaggtcg cccttgcaca
     1141 gggcgatgta gaagctgtgc ggcgagacct tggagcggaa gctgtgcacg gtccagccgt
     1201 gctcggcctc gacgccgagc agcttcgaga cgagcggggt gcgggcgaac tcctcgacca
     1261 gcagctcgcg ctgcaggcga tagccgatca ggtcggcgat cgtcaggatc gggaggtcgt
     1321 gcttgtgggc gaagcgctcg aggtcgtcca tgcgcgccat ctcgccgtcg tcgcgcatga
     1381 tctcgcagat gacgccggcg gggcgcaggc cggcgaggcg ggcgaggtcg accgagccct
     1441 cggtgtgacc cgagcggacc agcacgccgc ccggctgggc gcgcagcggg aagatgtggc
     1501 cggggctgac gatgtcggcg gcgccgaatt gcgcgtcggt gg
//
LOCUS       DASWNJ010001661         3884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_523247, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001661 DASWNJ010000000
VERSION     DASWNJ010001661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523247"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1389
                     /locus_tag="VGB85_27565"
     CDS             <1..1389
                     /locus_tag="VGB85_27565"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0137887.1"
                     /translation="NDEQRKQVQYIRRSSEELLQLVNDVLDLTRVEAGRTPLRADHFT
                     ASELIASLRGMLRPLLRADSPVRLIWDEPDPALELETDRAKLAQILRNLVANALKFTE
                     RGEIRVQTAVHGPFVRFTVRDTGIGIPEHALAGIFEEFTQVASHLQARVQGSGLGLPL
                     ARQLAEALGGEIDVESEPGRGSTFTVDVPRVLSEAAEVKTLEVKRATTPVGPASILVV
                     EDDRKTMLMYEKYLAVAGFHVVPARSIDAARAVLTTTRPVAIVLDIMLENETSWNFLG
                     ELKRDPATADIPVLVCTVTDRELKARALGADEFWLKPIDPDRLRRKLQTLTRPAQTTR
                     VLVIDDDERARYLMTKHLGETPYELHTAATGAEGVALARTCQPQLIFLDFLLREMTAF
                     DVLDQLKADPRTRKIPVVVVTSHVLEESDRRRLLTEAEAVLSKEHLSREVAIHRIRDA
                     LGKAGVAGTVSS"
     gene            1395..2921
                     /locus_tag="VGB85_27570"
     CDS             1395..2921
                     /locus_tag="VGB85_27570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0137888.1"
                     /translation="MNAPEVICNVNDREAPRYVITRMLTRAGFRVVEAVNGMEALAVA
                     RDERPAAIVLDLQLPDIDGMEVCRRLKADPATASIAVLQTSAAFISLEDKVHGLEHGA
                     DAFLAQPFTAQELIATLNSLLRIKERETTQLRRAEALAEADQRKDEFLAMLAHELRNP
                     LAAVTTAHAILDAYPARDSTEARARDIARRQAAQLGRIVDDLLDVSRMMRGIITLQRE
                     RVDLAALLGQVTTLASEVKLRGQDRKLTLSAPQPVLIDGDPTRLLQIFSNLIDNAIKF
                     TVPGGAIDVSLREADGMAVVCVRDDGVGMEPAALEQVFTLFFQAQTSIHRSRGGLGIG
                     LTLTRALAQLHGGSVVARSEGPGHGVEIEVRLPLAAADSTVDPRPEPAASTRAIRLLI
                     VEDNADVREILETLCDSWGHEVHVACDGLEGAAMALALLPDVALVDIGLPGIDGFEVA
                     RRIRADPRGSAIKLIALSGYGSPEQRAEGKAAGFDHHLVKPADPEHLAALLLSATEPA
                     "
     gene            complement(2924..>3884)
                     /locus_tag="VGB85_27575"
     CDS             complement(2924..>3884)
                     /locus_tag="VGB85_27575"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY0137889.1"
                     /translation="RSMRDGDKLVGWGMATGIWEAQQVPSVARAVLTGDGRLQVSSCS
                     ADIGPGTYTIMTQIAAETLGLPLADDSYSLGDSAMPQATIEGGSATASSVGSAVLAVC
                     ESVKKTLFKHARKLEGSPLASARIEDVVFVEREIRLRSDPSKAVSFRAAMRQGGLSCV
                     EEQTTAIPDPKRIKFTCNAHAAVFVEVKVDEALGEVTVTRVVSAVAGGRILNPKTARS
                     QILGGVVWGMGMALYEESLLDHRLGRFMNHNLAEYHVPVNADVRDIEVIFVEERDDVV
                     NPLGAKGLGEIGIVGVAAAIANAVFHATGKRIRDLPITLDKLL"
BASE COUNT          575 a         1550 c         1205 g          554 t
ORIGIN      
        1 aacgacgagc agcgcaagca ggtccagtac atccgccggt cgagcgagga gctgctgcag
       61 ctcgtcaacg acgtgctcga cctgacgcgc gtcgaggccg gccgcacccc gctgcgcgcc
      121 gaccacttca cggcgagcga gctgatcgcc tcgctgcgcg gcatgctgcg cccgctgctg
      181 cgcgccgaca gccccgtgcg gctgatctgg gacgagcccg accccgccct cgagctcgag
      241 accgaccgcg ccaagctggc ccagatcctg cgcaacctgg tcgccaacgc cctcaagttc
      301 accgagcgcg gcgagatccg cgtgcagacc gccgtgcatg ggccgttcgt ccgcttcacc
      361 gtgcgtgaca ccggcatcgg catccccgag cacgcgctcg ccggcatctt cgaggagttc
      421 acccaggtgg ccagccacct gcaggcccgc gtccagggca gcggcctcgg cctcccgctc
      481 gcgcgccagc tcgccgaggc cctcggcggc gagatcgacg tcgagagcga gcccgggcgc
      541 ggctcgacct tcaccgtcga cgtcccgcgt gtgctctccg aggccgccga ggtcaagacc
      601 ctcgaggtca agcgcgcgac cacccccgtc ggccccgcct cgatcctcgt cgtcgaggac
      661 gaccgcaaga ccatgctgat gtacgagaag tatctcgcgg tcgccggctt ccacgtcgtg
      721 cccgcgcgca gcatcgacgc cgcgcgggcc gtgctgacca ccacgcgtcc ggtcgcgatc
      781 gtgctcgaca tcatgctcga gaacgagacc tcgtggaact tcctcggcga gctcaagcgc
      841 gacccggcga ccgcggacat tccggtgctc gtgtgcaccg tcaccgaccg cgagctcaag
      901 gcgcgggcgc tcggcgccga cgagttctgg ctcaagccga tcgaccccga ccgcctgcga
      961 cgcaagctcc agaccctgac ccgacccgcg cagaccaccc gcgtgctcgt catcgacgac
     1021 gacgagcggg cccggtatct gatgaccaaa cacctcggcg agacccccta cgagctgcac
     1081 accgccgcga ccggcgcaga gggcgtcgcg ctggcccgca cctgtcagcc gcagctcatc
     1141 ttcctggatt tcctgctccg tgagatgacc gccttcgacg tgctcgacca gctcaaggcc
     1201 gacccccgga cccgcaagat ccccgtcgtc gtcgtcacct cgcacgtgct cgaggagtcc
     1261 gaccgccggc gcctgctgac cgaggccgag gccgtgctct ccaaggaaca cctgtcccgc
     1321 gaggtcgcca tccaccggat ccgcgacgct ctcggcaagg ccggcgtcgc cggcacggta
     1381 tcctcctgac tcccatgaac gcgccggagg tcatctgtaa cgtcaacgac cgcgaggccc
     1441 cgcgctacgt catcacgcgc atgttgaccc gcgccggctt tcgcgtcgtc gaggccgtca
     1501 acggcatgga ggccctcgcg gtcgcccgcg acgagcgccc cgcggcgatc gtcctcgacc
     1561 tccagctgcc cgacatcgac ggcatggagg tctgtcgccg cctcaaggcc gaccccgcga
     1621 ccgcctcgat cgccgtgttg cagacctcgg ccgccttcat cagcctcgag gacaaggtcc
     1681 acggcctcga gcacggcgcc gacgccttcc tcgcccagcc cttcaccgcc caggagctga
     1741 tcgccaccct caactcgctg ctgcggatca aagagcgcga gaccacccag ctgcgccgcg
     1801 ccgaggccct cgccgaggcc gaccagcgca aggacgaatt cctggcgatg ctcgcccacg
     1861 agctgcgtaa cccgctcgcc gcggtcacca ccgcccacgc gatcctcgac gcctaccccg
     1921 cgcgtgactc caccgaggcc cgcgcccgcg acatcgcccg ccgccaggcc gcgcagctcg
     1981 gccgcatcgt cgacgacctg ctcgacgtct cgcgcatgat gcgaggcatc atcaccctcc
     2041 agcgcgagcg cgtcgacctc gcggcgctgc tcggccaggt gaccacgctc gcctccgagg
     2101 tcaagctgcg cggccaggac cgcaagctga ccctgtccgc tccgcagccg gtcctcatcg
     2161 acggcgaccc gacccgcctg ctgcagatct tctccaacct gatcgacaac gcgatcaagt
     2221 tcaccgtgcc cggcggcgcg atcgacgtct cgctgcgcga ggccgacggc atggcggtcg
     2281 tgtgcgtgcg cgacgacggc gtcggcatgg agcccgcggc gctcgagcag gtcttcaccc
     2341 tgttcttcca ggcccagaca tcgatccacc gctcgcgcgg cggcctcggc atcggcctga
     2401 ccctgacccg cgcgctcgcc cagctgcacg gcggcagcgt cgtcgcccgc agcgagggcc
     2461 ccggccacgg cgtcgagatc gaggtccgtt tgccgctcgc cgccgcggac tcgaccgttg
     2521 accctcgccc ggagcccgcc gcctccacgc gggcgatccg cctgttgatc gtcgaggaca
     2581 acgccgacgt gcgcgagatc ctcgagaccc tgtgcgactc ctgggggcac gaggtgcacg
     2641 tcgcgtgcga cggcctcgag ggggcagcca tggcgctggc gctgctcccg gacgtcgcgc
     2701 tcgtcgacat cggcttgccg ggcatcgacg gcttcgaggt cgcgcggcgg atccgcgccg
     2761 acccacgtgg ttccgcgatc aagctgatcg cgctgtccgg ctacggctca ccggagcagc
     2821 gcgccgaggg gaaggccgcc ggcttcgacc accacctcgt caagcccgcc gaccccgagc
     2881 acctcgccgc cctgctgctc tccgcgaccg agccggcctg agctcacagc agcttgtcga
     2941 gggtgatcgg caggtcgcgg atgcgcttgc cggtcgcgtg gaacaccgcg ttggcgatcg
     3001 ccgccgcgac cccgacgatc ccgatctcgc cgagcccctt ggccccgagc gggttgacca
     3061 cgtcgtcgcg ctcctccacg aagatcacct cgatgtcgcg cacgtccgcg ttcaccggca
     3121 catgatactc cgcgaggtta tggttcatga agcggccgag gcggtggtcg agcaggctct
     3181 cctcgtagag cgccatgccc atgccccaca ccacgccgcc gaggatctgg ctgcgcgccg
     3241 tcttcgggtt caagatccgc ccgcccgcga ccgcgctgac gacccgcgtc accgtcacct
     3301 cgccgagcgc ctcgtcgacc ttgacctcga cgaacaccgc cgcgtgcgcg ttgcaggtga
     3361 acttgatccg cttcgggtcc ggtatcgccg tcgtctgctc ctcgacgcat gacagcccgc
     3421 cctgccgcat cgccgcccga aagctgaccg ccttcgacgg gtcggatcgc agccggatct
     3481 cgcgctcgac gaacacgaca tcctcgatcc gcgcgctcgc cagcggtgag ccctcgagct
     3541 tgcgcgcgtg tttaaaaagg gtcttcttga ccgactcgca caccgccagc accgccgagc
     3601 cgaccgacga ggcggtcgcc gagccgccct cgatcgtcgc ctgcggcatc gccgagtcgc
     3661 cgagcgagta gctgtcgtcc gccagcggca gccccagcgt ctccgccgcg atctgcgtca
     3721 tgatcgtgta tgtgccgggg ccgatgtcgg cgctgcagct gctgacctgc aacctgccat
     3781 cgcccgtcag caccgcgcgc gcgaccgagg gcacctgctg ggcctcccag atgccggtcg
     3841 ccatgcccca gccgacgagc ttgtccccgt cgcgcatcga tcgc
//
LOCUS       DASWNJ010001662         1869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_699355, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001662 DASWNJ010000000
VERSION     DASWNJ010001662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699355"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VGB85_27580"
     CDS             complement(<1..730)
                     /locus_tag="VGB85_27580"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0137890.1"
                     /translation="MSREIPLAPQHLPILGHIAPMLMGPPWDITEQWLRTHGPTLRFK
                     IPGAEYIVSSDTEVVRQVLLTNADNYVKDIRSMAPFLDLLGQGLLSSDGPLWKRQRAT
                     LAKAFRIDALRNVADVTRRAVDRTSVTLDKHARSGEPVDIGALFRRLTLQVIAEATLS
                     MTPEESDEVLPRLYEPLVAEANRRVWLPMRARLPLPARFHYDRSVRELNVFLAAKIDE
                     RQRQHRADPKRPATDMLDMLLRSFA"
     gene            939..>1869
                     /locus_tag="VGB85_27585"
     CDS             939..>1869
                     /locus_tag="VGB85_27585"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0137891.1"
                     /translation="MKRHYAVGLAVAVAAGCNEPGDDLGGSLPRIECEIGIVGGGVAG
                     TYMAYRLTPEYGDKVCVFEKEPELGGRTRDTTLDGKPDSPRVGTGARRIMENQASMFA
                     LAEELGVEFEKPEIGTDLINAREHFAYTRDAFLPLYPGVEGPLDEDDATDRETELYEL
                     LRHGPERANAAEYADFRTYIRAVAGEEELEFLRDMSRFRGDFDYPLAVENYFSWLDEE
                     WDTCCTPSYPVGGMSAFIVAMEKRAVAAGGRIFKSEPVLRISREDQGYLLRTDAQEVK
                     VQKLIIAAPPDGVDHIEGEVAEQVRERPEYRALL"
BASE COUNT          286 a          611 c          694 g          278 t
ORIGIN      
        1 tggcgaagct gcgcagcagc atgtcgagca tgtcggtcgc gggccgcttc gggtcggccc
       61 ggtgctggcg ctggcgctcg tcgatcttcg ccgccaaaaa tacgttgagc tcgcgcaccg
      121 agcggtcgta atgaaagcgg gccggcagcg gcaggcgggc gcgcatcggc agccacacgc
      181 ggcggttggc ctcggcgacc aagggctcgt agagccgcgg caggacctcg tcgctctcct
      241 cgggcgtcat cgacagggtc gcctcggcga tcacctgcaa ggtcaggcgg cgaaacagcg
      301 cgccgatgtc gaccggctcg ccgctgcgcg cgtgcttgtc gagcgtgacg ctcgtgcggt
      361 cgacggcgcg gcgcgtgaca tcggcgacgt tgcgcagcgc gtcgatgcga aacgccttgg
      421 cgagggtcgc ccgctggcgc ttccacagcg gcccgtcgct gctgagcaga ccctgcccga
      481 gcaggtcgag gaacggcgcc atcgaccgga tgtctttgac gtaattgtcg gcgttggtca
      541 gcaacacctg gcgcacgacc tcggtgtcgg agctgacgat gtactcggcg ccggggatct
      601 tgaagcgcag cgtcggaccg tgcgtgcgca gccactgctc ggtgatgtcc cacggcggac
      661 ccatgagcat cggggcgatg tggccgagga tcggcagatg ctgcggtgcg agcgggatct
      721 ctcgggacat cggggggcgc aagtgtcgtt gacccgcgcc cggcgacgca agggcggacc
      781 gcatgcgcgc ccacacccca tgcgggccgt ccgccgtgcg gagccgatct cgtgaccgcg
      841 actgtggaca cacccggggc tccgtgtggt cgctgtgcat cggccgaggg taccctcggg
      901 tggcgcgctc caggaccgtg caggtatgct tcgcggcgat gaagcggcat tatgcagtgg
      961 gtctggcggt ggctgtggcg gcgggctgca acgagcccgg ggacgacctc gggggctccc
     1021 tcccgcggat cgagtgcgag atcgggatcg tcggtggcgg ggtcgccgga acctatatgg
     1081 cctaccgcct gacccccgag tacggcgaca aggtctgcgt gttcgagaag gagcccgagc
     1141 tcggcgggcg cacgcgcgac accaccctcg acggcaagcc cgacagcccg cgcgtcggca
     1201 ccggcgcgcg ccggatcatg gagaaccagg cctcgatgtt cgctctggcc gaggagctcg
     1261 gcgtcgagtt cgaaaagccc gagatcggca ccgacctgat caacgcccgc gagcacttcg
     1321 cgtacacgcg tgacgccttc ttgccgctgt acccgggggt cgaggggccg ctcgacgagg
     1381 acgacgcgac cgaccgcgag accgagctct acgagctgct gcgccacggg cccgagcggg
     1441 ccaacgccgc cgagtacgcg gactttcgca cgtacatccg cgcggtcgcc ggcgaggagg
     1501 agctcgagtt cctgcgcgac atgtcgcgct ttcgcggcga cttcgactac ccgctggcgg
     1561 tcgagaacta cttcagctgg ctcgacgagg agtgggacac ctgttgcacg ccctcgtacc
     1621 cggtcggtgg aatgagcgcg ttcatcgtgg cgatggagaa gcgcgcggtg gcggcgggcg
     1681 gacggatctt caagtccgag ccggtgctcc ggatctcgcg cgaggatcag ggctatctgc
     1741 tgcggacgga cgcgcaggag gtcaaggtcc agaagctgat catcgcggcg ccgccggacg
     1801 gcgtcgacca catcgagggc gaggtcgcgg agcaggtccg cgagcgcccc gagtatcgcg
     1861 cgctgctgc
//
LOCUS       DASWNJ010001663         5218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_665954, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001663 DASWNJ010000000
VERSION     DASWNJ010001663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665954"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="VGB85_27590"
     CDS             complement(<1..970)
                     /locus_tag="VGB85_27590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137892.1"
                     /translation="MDLSTLLAREGIEAGELDALVKWARHRATALVARIEPGSPLAEA
                     LGAALERTRDANGTPVARPAPPSAPRPIARAVSKPYLGAAVAAALSGKQEDLDLALET
                     AAADAEADDLDLEMPVLEDSPPAKPTPVAAASAPASVASASAPAPVVAAAPEPEPELD
                     ENDPSIGGFNRFAFSYRRREAERAAAEESRSQMALAREAASHAEDTATSGEWTVAALE
                     LPEPPTFIAKVTRQRQPGTDPGDAEASGLLVLGIPDEEGIDIPVQRQRSSVRYADSRP
                     FESFTPPTPEVFERSEPQRVTHAPSRPHPTPEVHELRPVLDDEDSFD"
     gene            complement(1194..3881)
                     /gene="alaS"
                     /locus_tag="VGB85_27595"
     CDS             complement(1194..3881)
                     /gene="alaS"
                     /locus_tag="VGB85_27595"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEY0137893.1"
                     /translation="MQSRPRAHEIRRRFLDFFAGRGHAVVPSSSLVPSNDPTLLFTNA
                     GMVQFKDTFLGRDPRPYKRAVSVQRCLRAGGKHNDLDNVGFTDRHHTLFEMLGNFSFG
                     DYFKQDAVKWGWEFLTQDLGIPADKLVVSVFSGEGESAPADDEAYQLWTAYLPEDRIY
                     RLPAKENFWAMGDTGPCGPCSEIHIFHGDRAPGDAKRDGKLGPAYEDTRYTELWNLVF
                     MQYEKLPDGSLVSLPRPSIDTGAGLERLAAVMEGVGSNYRTSLLTPLVDLAKRLAGAA
                     MDDLGAGESPFRVIADHARATAFLIADGVFPDKTGRSYVLRRIMRRAIRYGNNVGLEK
                     AFFHEICRKVVEDFGEAYPQLIERQGTIDEVVRIEEDAFRRTLSRGLKRLEAALTDLD
                     PQHASFPAAVAADLYDTYGFPVDVTGIIVKEKGLTLDEEAVEAAIRQRQHQDGGAAGS
                     LGSDKAIGDLYFKLKEKHAPTAVFSGYAATTGTGTVLALAVDGKEADEAQAGAQVEFV
                     VDSTPFYAESGGQIGDTGRAFADGLELEITDTHKPTGDVHVHRGQLRSGTLRVGQQIT
                     LEVDGERRAAIRRNHSATHLLHHALRAVLGTHVAQKGSLVAPDRLRFDFSHTRPLTLE
                     QRQEIERRVNAEVLRNKDSVIRNLSMDDARKTGAIGLFEAKYGEVVRVISIGGESVEL
                     CGGTHVDRAGDIGLFAIVSEAGIAQGVRRIEAVTGMNAVAYLQQTTQILGEAASQLHA
                     AGPHEVLAKLERLRQDIRVREREISELQRKLTVGSGGGAGDTVIEVAGVKLLTKLVTV
                     GDPKALREAADALRDRLGSGVVVLGAEREGKANLLVAVTKDLQGKIHAGNLIAAIVGH
                     VEGRGGGRPELAQAGGANPAGLPKALESAREALAAQLGS"
     gene            complement(3996..>5218)
                     /locus_tag="VGB85_27600"
     CDS             complement(3996..>5218)
                     /locus_tag="VGB85_27600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEY0137894.1"
                     /translation="TPLSRRPARPTVRGPSVKVPLDVARLVRAGHPWVFRHTVRRDLD
                     NLAEGTVVPVMDEDGYGVGWGLVESEGAIGVRMLSLAPDFNWDEAEMVSRIRLALRLR
                     GPELLAQGAVRLLHGESDGFPGLAVDRLGDYLLVYKYARVAESYLDALIPLLEQELKP
                     TGIYMQDRIRSVTADDRRPPAFHLAGKAAPPEIEVAEDGLKFLVDVTAPVSPGLFLDL
                     REGRRLSEQWVKGRSVCNLFSFTGAFGLRAIRAGATTVTHVDAAARSHSRCRQNLAAS
                     GMDPEGIEAIHGDVFTLLSKFRQRNRVFDFIVVDPPPFSNVKGSTFSALKDWNQLMAS
                     VAGVVSPGGHVLAISNAVRLPEDEFFLALGEGVHAAKRSARLVGECGLPPDFPVPPAF
                     NEGRYLKIKLLQLD"
BASE COUNT          714 a         1710 c         2007 g          787 t
ORIGIN      
        1 gatcgaagga gtcctcgtcg tcgagcacgg gccgcagctc gtggacctcg ggggtcgggt
       61 gcgggcgcga gggcgcatgc gtcacgcgct gcggctcgga ccgctcgaag acctccgggg
      121 tcggcggggt gaagctctcg aacgggcgcg agtcggcgta gcggacgctg ctgcgctggc
      181 gctggaccgg gatgtcgatg ccctcctcgt ccgggatgcc gagcacgagc aggccggagg
      241 cctcggcgtc gccggggtcg gtgccgggct ggcgctggcg ggtgaccttg gcgatgaagg
      301 tgggcggctc gggcagctcg agcgcggcga cggtccactc gccggaggtg gcggtgtcct
      361 ccgcgtggct ggcggcttcg cgcgcgaggg ccatctgcga ccggctctcc tcggccgcgg
      421 cccgctcggc ctcgcggcgg cggtagctga aggcgaagcg attgaaccca ccgatcgagg
      481 ggtcgttctc gtcgagctcg ggctccggct ccggcgcagc agcgaccacg ggcgcgggtg
      541 cggaagcaga tgcgacagat gcgggtgcag atgcggctgc gacaggggtc ggcttcgcgg
      601 gtgggctgtc ctcgaggaca ggcatctcga gatcgaggtc gtcggcctcc gcgtcggcgg
      661 cggctgtctc gagggccagg tcgaggtcct cctgcttacc gctcagcgcg gccgccacgg
      721 cggcgccgag gtacggcttg ctgacggcgc gggcgatcgg gcggggagcg ctcggcgggg
      781 ccgggcgggc gacgggcgtg ccgttggcgt cacgcgtgcg ctcgagcgcg gcgccgagcg
      841 cctcggcgag cggcgagccg ggctcgatgc gggcgacgag cgccgtggcg cgatgcctgg
      901 cccacttgac cagggcgtcg agctcaccgg cctcgatgcc ctcgcgggcg agcaacgtgc
      961 tcaagtccat gccgagtcca agttatcaca ggcgccgcta cgaacgcgag aggcccctgt
     1021 ccccccgcgt acgacgtttt cgaagttaac gtcgatcagt ccgcaactgc ttcctgtagg
     1081 cacggggcgg ccgcgtgggt cgggcggtca cctcgccgcc gcggccggtc ggccacagcg
     1141 gtcacattcg ccgagtttgc agcgatgccg tgcgtcgggc gacgcaccgg gctttagctg
     1201 ccgagctgcg ccgcgagggc ctcgcgggcg gactcgaggg ctttcggcag tccggccggg
     1261 ttggccccgc cggcctgcgc gagctcgggg cgaccaccac cacgaccctc gacatgaccg
     1321 acgatcgcgg cgatcaggtt gccggcgtgg atcttgccct gaaggtcctt ggtgacggcg
     1381 accagcaggt tggccttgcc ctcgcgctcg gcgccgagga cgacgacgcc ggagccgaga
     1441 cggtcacgca gggcgtcggc cgcctcgcgc agcgccttgg ggtcgccgac ggtgacgagc
     1501 ttggtcagca gcttgacgcc ggcgacctcg atgacggtgt cccctgcccc gccgccgctg
     1561 ccgacggtca gcttgcgctg cagctcgctg atctcgcgct cacggacgcg gatgtcctgg
     1621 cgcaggcgct cgagcttggc gagcacctcg tgcgggcccg cggcgtgcag ctggctcgcg
     1681 gcctcgccga ggatctgcgt ggtctgctgc aggtacgcga ccgcgttcat gccggtgacg
     1741 gcctcgatgc gccgcacgcc ctgggcgatg ccggcctcgc tgacgatcgc gaacaggccg
     1801 atgtcgccgg cgcggtcgac gtgggtgccg ccgcacagct cgacgctctc gccgccgatg
     1861 ctgatcacgc gcacgacctc gccgtacttg gcctcgaaca ggccgatcgc gccagtcttg
     1921 cgggcgtcgt ccatcgacag gttgcggatc acgctgtcct tgttgcgcag gacctcggcg
     1981 ttgacgcgac gctcgatctc ctggcgctgc tcgagcgtca gcgggcgcgt gtgcgagaag
     2041 tcgaagcgca ggcggtcggg ggcgaccagc gagcccttct gggcgacgtg ggtgccgagc
     2101 acggcccgca gcgcgtgatg cagcaggtgg gtcgcgctgt ggttgcggcg gatcgctgcg
     2161 cgccgctcgc cgtcgacctc gagggtgatc tgttgcccga cgcgcagggt gccgctgcgc
     2221 aactgaccac ggtgcacgtg cacgtcgccg gtcggcttgt gcgtgtcggt gatctcgagc
     2281 tcgaggccgt cggcgaaggc gcggccggtg tcgccgatct ggccgccgct ctcggcgtag
     2341 aacggggtgc tgtcgacgac gaattcgacc tgcgcgccgg cctgggcctc gtcggcctcc
     2401 ttgccgtcga cggcgagcgc gagcacggtg ccggtgccgg tggtcgccgc gtaaccgctg
     2461 aagacggccg tcggggcgtg cttctccttg agcttgaagt acaggtcgcc gatcgccttg
     2521 tcgctgccga gcgaacccgc ggcgccgccg tcctggtgct ggcgctggcg gatcgcggcc
     2581 tcgacggcct cctcgtcgag ggtcaggccc ttctccttga cgatgatgcc ggtgacgtcg
     2641 accgggaagc cgtaggtgtc atagaggtcg gcagcgacgg ccgccgggaa gctggcgtgc
     2701 tgcgggtcga ggtcggtgag cgcggcctcg aggcgcttga ggccgcggct gagcgtgcgg
     2761 cgaaacgcgt cctcctcgat gcgcacgacc tcgtcgatcg tgccctggcg ctcgatgagc
     2821 tgcgggtagg cctcgccgaa gtcctcgacg accttgcggc agatctcgtg gaagaaggcc
     2881 ttctcgaggc cgacgttgtt gccgtagcgg atcgcgcgac gcatgatgcg gcgcagcacg
     2941 tacgagcggc cggtcttgtc cgggaacacg ccgtcggcga tcaggaaggc ggtcgcgcgc
     3001 gcgtggtcgg cgatgacgcg gaaggggctc tcgccggcgc cgaggtcgtc catcgcggcg
     3061 cccgcgaggc gcttggcgag gtcgacgagc ggggtcagca gcgaggtgcg gtagttgctg
     3121 ccgacgccct ccatgacggc ggcgaggcgc tcgaggccgg cgccggtgtc gatgctcggg
     3181 cgcggcagcg agacgaggct gccgtcgggc agcttctcgt attgcatgaa cacgaggttc
     3241 cacagctcgg tgtagcgggt gtcctcgtag gcggggccga gcttgccgtc acgcttggcg
     3301 tcgccggggg ccctgtcgcc gtgaaagatg tggatctccg agcacgggcc gcacgggccg
     3361 gtgtcgccca tcgcccagaa gttctccttg gcgggcaggc gatagatgcg gtcctcgggg
     3421 aggtacgcgg tccacagctg ataggcctcg tcgtcggccg gcgccgactc gccctcgccg
     3481 ctgaacaccg agacgacgag cttgtccgcc gggatgccga ggtcctgggt caggaactcc
     3541 cagccccact tgacggcgtc ctgcttgaag tagtcgccga acgagaagtt gccgagcatc
     3601 tcgaacaggg tgtggtgacg gtcggtgaag ccgacgttgt cgaggtcgtt gtgcttgccg
     3661 ccggcgcgga ggcagcgctg caccgacacg gcgcgcttgt acggacgcgg gtcgcgaccg
     3721 aggaaggtgt ccttgaactg gaccatgccc gcgttggtga acagcagggt cgggtcgttg
     3781 ctgggcacca gcgaggacga cggcacgacg gcgtgaccac gaccggcgaa gaagtcgagg
     3841 aaacggcgac ggatctcgtg ggcgcggggc cgggactgca tggggcgccg aggataaacg
     3901 gctttgcccc gcacgtgcgc cgcggaggct ttgtcggacc gggcgcggga ggagtgagct
     3961 tcctccggag cttcctccga agatgtgagc gcggctcagt cgagttgcag cagcttgatc
     4021 ttcaggtagc ggccctcgtt gaaggccggc gggaccggga agtcgggcgg gaggccgcac
     4081 tcgccgacca aacgggccga gcgcttggcg gcgtggaccc cctcaccgag cgccaggaag
     4141 aactcgtcct ccggcaggcg cacggcgttg gagatcgcca ggacgtggcc gccggggctg
     4201 acgacgccgg cgaccgacgc catcagctgg ttccagtcct tgagggcgct gaaggtcgag
     4261 cccttgacgt tggagaacgg cggcgggtcg acgacgatga agtcgaacac gcggttgcgc
     4321 tggcggaact tgctcagcag cgtgaacacg tcgccgtgga tcgcctcgat gccctcgggg
     4381 tccatgccgg aggcggcgag gttctgccgg caacgcgagt ggctgcgcgc ggcggcgtcg
     4441 acgtgggtca ccgtggtcgc gccggcgcgg atcgcccgga gaccaaaagc gccggtgaag
     4501 ctgaagaggt tgcacaccga gcggcccttg acccactgct ccgacaacct tcgcccctcg
     4561 cgcaggtcga ggaacaggcc gggcgagacc ggggcggtga cgtcgacgag gaacttgagg
     4621 ccgtcctcgg cgacctcgat ctccggcggc gcggccttgc cggcgaggtg gaacgcgggc
     4681 gggcggcggt cgtcggcggt gaccgaacgg atccggtcct gcatgtagat gccggtcggc
     4741 ttgagctcct gctcgagcag ggggatcagc gcgtcgaggt agctctcggc gacgcgggcg
     4801 tacttgtaga cgagcaggta gtcgccgagg cggtcgaccg cgaggcccgg gaaaccatcg
     4861 gactcaccat gcaacaggcg cacggcgcct tgcgccagca gctccgggcc gcgcaaacgt
     4921 agcgcgagcc ggatccggct gaccatctcg gcctcgtccc agttgaagtc gggcgcgagg
     4981 ctcaacatgc gcacaccgat cgcaccctcg gactcgacga gaccccagcc gacgccgtag
     5041 ccgtcctcgt ccatcaccgg gacgaccgtg ccctcggcga ggttgtcgag gtcgcggcgc
     5101 acggtatgac gaaataccca cgggtggccg gcgcggacca gacgggcgac gtccagcggg
     5161 accttgacgc tggggccacg caccgtcggc cgcgcgggcc ggcgactcag cggcgtgt
//
LOCUS       DASWNJ010001664         4667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_488884, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001664 DASWNJ010000000
VERSION     DASWNJ010001664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488884"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VGB85_27605"
     CDS             complement(<1..190)
                     /locus_tag="VGB85_27605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137895.1"
                     /translation="MLRHDARQWLPGVLGLGLMTAACGVLASACGDNGGSFGATEGDS
                     LSGGAGASTGNGDDGGQQP"
     gene            complement(359..2281)
                     /locus_tag="VGB85_27610"
     CDS             complement(359..2281)
                     /locus_tag="VGB85_27610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0137896.1"
                     /translation="MTTPGSTPPEDPAYDRAACGLLTTARDGTIQRVNTTFCAWTGHS
                     AAELLGRRFQELLTIGSKLFHQTHWMPLLQMQGSVAEVQLEIVHRDGRVLAVLVNAAR
                     RRVPDGTTADGTVDIAVFIATDRRKYERELLAARRRAEELLASEREAQAARVLAEARL
                     RLALESAQLRVWSADLPGGVRRYERDVAALLGAPELAEVDAAAYVACIHPDDRATEAQ
                     AFAVAVDPAERASYSVEYRLLGRDGVERIVRSTGRAFFGGDGVASQFSGVLEDVTSHR
                     RAHEALRQREIEFRALAENSPDIITRIDRQHRYLYMSPSAEPLAGRPVAAMLGKRVDE
                     VTLGPEVGAQWIAAIDDAFRGRDGTVSFCHHGADGVRRDLQARFVAERDTTDEVRSVL
                     AITRDVTALRRQERDAEQRALLAEQLIGIVSHDLRNPLNAVLLGTHLLGANDPEVQRR
                     TRARITSAAQRATRLVNDLLDFTQAKLGGGIRVAPREVQLHTLVGDCLDEVRLAWPGR
                     ALEHRRIGDGVALADPDRLAQVVTNLGNNALTYGAPEVPVTFTTMLDEHRVEIHVHNG
                     GPPIPRELQATIFEPMKRGEDKLRLGSRSVGLGLYIVGEIAAAHGGRVRVRSEEGEGT
                     TFIVSIPRDSAGARVP"
     gene            complement(2283..3071)
                     /locus_tag="VGB85_27615"
     CDS             complement(2283..3071)
                     /locus_tag="VGB85_27615"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0137897.1"
                     /translation="MDLTTRHNIHVIGRGETTLLFAHGYGCDQNMWRLVTPAFKDRFR
                     IILYDLVGSGGSDLSAYDREKYSSLEGHARDVVEIATELSPGPVIFIGHSVSAMIGML
                     AGIAAPERFISQVMIGPSPCYINDGDYVGGFTRADIESLLDTLDSNYLGWASAMAPTI
                     MGAPDRPELGQELTNSFCRTDPEIAKHFARVTFLSDNRADIPRFTTRALILQCSEDII
                     APPSVGAYLHRMLPGSALRVVDNIGHCPHLSAPDDIVAAITDFV"
     gene            3493..>4667
                     /locus_tag="VGB85_27620"
     CDS             3493..>4667
                     /locus_tag="VGB85_27620"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY0137898.1"
                     /translation="MPDHDDRPDPAASAAPWWQRGVIYQIYPRSFQDSDGDGVGDLRG
                     VVRRLAYLQELGVDAVWISPFYASPMADFGYDVTDHGAVDPVFGTLADFEAVIREAHA
                     RDIRVILDYIPNHTSALHPWFLEARASRRAPRRDWYVWRDPAPDGGPPNNWRSAFGGS
                     AWSLDETTGQYYLHTFLKEQPDLNWRNPAVEREMLGVLRCWLDRGADGVRVDAIQVVL
                     KDLELLDNPPNPDYREGIDDPYDALLRVHSSDRPEIHALVGRMRALVDSYPGERVLIG
                     EIYNLVERVVAYYGVDGRGVHFPYNFQLIKLPWDARTIDAAVRRYEALLPPGAWPNWV
                     LGNHDRSRVASRVGPAQARVAAMLLLTLRGTPTIYYGDELGIADVPIPVDRVRDPWER
                     "
BASE COUNT          696 a         1609 c         1650 g          712 t
ORIGIN      
        1 tcggctgctg gccgccgtcg tcgccgttgc cggtcgaggc cccggccccg ccgctcagcg
       61 agtcgccctc ggtcgcgccg aagctaccac cgttgtcgcc gcaggccgaa gccagcacgc
      121 cgcaggccgc ggtcatcagg cccaggccga gcaccccggg caaccactgc cgggcatcat
      181 gtcgcaacat cgtagcctcc gtcgttcaag gtcaagtcaa gtcaagaaga ccctcacgat
      241 actccggcgc gaaccgcctg gcaagccgcg gcggcggcga agcatgcgtc cgggaatgct
      301 gttttgctgg tttgccgcag cgaagtcctg cgctgtatct ccacgagagg gtgtgcgatc
      361 acgggacacg ggcgcccgcg gagtcgcggg ggatcgatac gatgaaggtc gtgccctcgc
      421 cctcctccga gcggacgcgg acgcggccgc cgtgcgccgc tgcgatctcg ccgacgatgt
      481 agagcccgag cccgacgctg cgcgagccga gccgcagctt gtcctcgccg cgcttcatcg
      541 gctcgaagat ggtcgcctgc agctcgcgtg ggatcggcgg gccgccgtta tggacgtgga
      601 tctcgacgcg atgctcgtcg agcatcgtgg tgaaggtgac gggcacctcc ggagcgccgt
      661 aggtcagcgc gttgttgccg aggttggtga cgacctgcgc gagccggtcg gggtcggcga
      721 gcgcgacgcc gtcgccgatc cgccggtgct cgagtgcgcg acccggccag gcgagccgga
      781 cctcgtcgag gcagtcgccg acgagcgtgt gcagctgcac ctcgcgcggc gcgacccgga
      841 tcccgccgcc gagcttggcc tgcgtgaagt cgaggaggtc gttgacgagg cgggtcgccc
      901 gttgcgcggc cgaggtgatc cgggcccggg tgcgccgctg cacctcgggg tcgttcgcgc
      961 cgagcaggtg cgtcccgagc agcacggcgt tgagcgggtt tcgcaggtcg tggctgacga
     1021 tgccgatcag ctgctcggcg agcaatgccc gctgctcggc gtcacgctcc tgccggcgca
     1081 gcgccgtgac gtcgcgggtg atcgccagca ccgagcggac ctcgtcggtc gtgtcgcgct
     1141 cggcgacgaa gcgggcctgg aggtcgcgcc gcacgccgtc ggccccgtgg tggcaaaacg
     1201 agaccgtgcc gtcgcgcccg cgaaacgcgt cgtcgatcgc ggcgatccac tgcgcgccga
     1261 cctcggggcc gagcgtgacc tcgtcgacgc gcttgccgag catcgccgcc acgggccggc
     1321 ccgcaagcgg ctcggccgac gggctcatgt acagatatcg gtgctggcga tcgatccgcg
     1381 tgatgatgtc gggtgaattc tcggcgagcg cacgaaactc gatctcgcgc tggcgcagcg
     1441 cctcgtgggc ccggcgatgg ctggtgacat cctcgagcac gcccgagaac tgcgaggcga
     1501 ccccgtcgcc cccgaagaac gcgcggccgg tggaacgcac gatgcgctcg acgccgtcgc
     1561 gcccgagcag gcgatactcg accgagtacg aggcgcgctc cgccgggtcg accgccacgg
     1621 cgaaggcctg cgcctcggtc gcccggtcgt ccgggtggat gcacgcgaca tacgcggcgg
     1681 cgtcgacctc cgcgagctcg ggcgcgccga gcagcgcggc gacgtcacgc tcgtagcggc
     1741 gcacgccgcc gggcaggtcg gccgaccaga cgcgcagctg cgcggactcg agggcgaggc
     1801 gcaggcgggc ctcggcgagc acgcgggcgg cctgggcctc gcgctcgctg gcgagcagct
     1861 cctcggcccg ccggcgcgcg gcgagcagct cgcgctcgta cttgcggcgg tcggtggcga
     1921 tgaacaccgc gatgtcgacc gtgccatcgg cggtggtgcc atcgggcacg cggcgccggg
     1981 ccgcgttgac gagcaccgcg agcacgcggc cgtcgcggtg cacgatctcc agctggacct
     2041 cggcgaccga tccctgcatc tgcagcagcg gcatccagtg ggtctgatga aacagcttgc
     2101 tgccgatcgt cagcagctcc tggaagcgcc ggccgagcag ctccgccgcg ctgtgcccgg
     2161 tccacgcgca aaaggtcgtg ttgacgcgct ggatcgtgcc gtcgcgcgcg gtcgtcagca
     2221 gaccgcaggc agcacgatcg tacgcggggt cctcgggcgg agtggagccg ggcgtggtca
     2281 cgtcacacga agtcggtgat cgccgccacg atgtcgtcgg gcgcgctgag gtgcgggcag
     2341 tggccgatgt tgtcgaccac gcgcagggcg ctgccgggca gcatgcgatg caggtacgca
     2401 ccgacgctcg gcggcgcgat gatgtcctcc gagcattgta agatcagcgc gcgggtcgtg
     2461 aacctcggga tgtcggcccg gttgtcgctg aggaaggtga cgcgcgcgaa gtgcttggcg
     2521 atctccgggt cggtccgaca aaagctgttc gtcagctcct gaccgagctc ggggcggtcg
     2581 ggtgcgccca tgatcgtcgg cgccatcgcg ctcgcccacc cgaggtaatt gctgtccagc
     2641 gtgtcgagca acgattcgat gtcagcccgc gtgaagccgc cgacatagtc gccgtcgttg
     2701 atatagcaag gagacggccc gatcatcacc tgcgagatga agcgctccgg cgcggcgatc
     2761 ccggcgagca tcccgatcat cgcgctcacc gagtggccga tgaagatcac cgggcccggc
     2821 gacagctcgg tcgcgatctc gacgacgtcg cgggcgtgcc cctcgagcga gctgtacttc
     2881 tcgcggtcgt atgccgacag gtccgagccg cccgaaccga cgagatcgta aagaatgatg
     2941 cgaaaccggt ccttgaacgc cggcgtcacc agccgccaca tgttttggtc gcacccgtag
     3001 ccgtgcgcga acagcagggt cgtctcgccg cgaccgatca cgtggatgtt gtgccgcgtc
     3061 gtgagatcca tggtgcgacg ctaccgcagc cgtcgccgcg cggggggcga aatcgtgtcc
     3121 tcatgtgcgg acctgtggtc ggcgacacat gatcgatgga cggatgaggc gagtgccgtc
     3181 gtcgccagcg ggtgaccgtg atactgccag tggtacgatg ctccgggcgc gtcgcacccc
     3241 accgatcggt cggcgagtac gacctgcgtc gctcgatgct cgatgcgctc ggcgcgagga
     3301 gcgtgagcaa caagatgcct cctctggtat atccgctggt acgcgaacgc ctgggaccgc
     3361 gtacatgtgc agcaggcgag ggcgactatc cgctcgagat ccgggggccg caattgctcg
     3421 cggccatctc gcgctcgtgg accctgaagc gcttgacgaa gtccggcgcc gcgaccacat
     3481 acgcggagcg tcatgcccga tcacgacgac agacccgacc ccgcagcgtc cgcagcgcca
     3541 tggtggcagc gcggcgtcat ctaccagatc tacccgcggt cgttccagga cagcgacggc
     3601 gacggcgtcg gcgacctgcg aggggtcgta cgccggctgg cgtatcttca ggagctcggc
     3661 gtcgacgcgg tgtggatcag cccgttctac gcctctccga tggcggactt cggctacgac
     3721 gtcactgacc acggcgcggt cgacccggtg ttcgggaccc tcgccgactt cgaggcggtc
     3781 atccgcgagg cccacgcccg cgacatccgc gtgatcctcg actacatccc caaccacacg
     3841 tcggcgctgc acccgtggtt cctcgaggcc cgcgcgtcgc ggcgcgcgcc gcggcgtgac
     3901 tggtacgtat ggcgcgaccc ggctcccgat ggcggcccgc ccaacaactg gcgcagcgcc
     3961 ttcggcggca gcgcgtggtc cctcgacgag acgaccgggc aatactatct ccacactttt
     4021 ttaaaggagc agccggacct caactggcgc aaccccgcgg tcgagcgcga gatgctcggc
     4081 gtgctgcgct gctggctcga ccgcggcgcc gacggagtgc gggtcgacgc gatccaggtc
     4141 gtgctcaagg atctcgagct tcttgataat ccgccgaacc cggattaccg cgagggcatc
     4201 gacgatccat acgacgcgct gctgcgcgtg cactcgtcgg accgcccgga gatccacgcg
     4261 ctcgtcggcc ggatgcgggc gctggtcgac agctatcccg gcgagcgcgt gttgatcggg
     4321 gagatctata atctcgtcga gcgggtcgtc gcatattatg gcgtcgatgg ccgcggggtg
     4381 cactttccct acaactttca gttgatcaag ttaccgtggg acgcccgcac gatcgacgcc
     4441 gcggtgcgcc gctatgaagc tctgctcccg cccggcgcct ggcccaactg ggtgctcggc
     4501 aaccacgacc gctcgcgcgt ggcgagccgc gtcggtcccg cccaggcgcg ggtcgccgcg
     4561 atgctgctgc tgaccctgcg cggcacaccg acgatctatt atggcgacga gctcggcatc
     4621 gccgatgtgc cgatcccggt cgaccgcgtc cgggacccat gggagcg
//
LOCUS       DASWNJ010001665         1693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_699419, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001665 DASWNJ010000000
VERSION     DASWNJ010001665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699419"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VGB85_27625"
     CDS             <1..326
                     /locus_tag="VGB85_27625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137899.1"
                     /translation="PIGEAKLRAAGVQDEAWFEDLTMIPERPEVFIMGRARGVEVFLS
                     QSLGGVEVLRCRPRGRPSEIEPGGRWLAIEAWRQGERIDLAEARPHIAASLDAVAKGG
                     VLASV"
     gene            422..664
                     /locus_tag="VGB85_27630"
     CDS             422..664
                     /locus_tag="VGB85_27630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137900.1"
                     /translation="MEAGDVTVCLDCASIAVCDKVTRTGRPVLRKPTGKEKAVFRQDP
                     RLGQVQAAIIMQARQSGRELRDTPPVVRPAPSGTRQ"
     gene            complement(782..>1693)
                     /locus_tag="VGB85_27635"
     CDS             complement(782..>1693)
                     /locus_tag="VGB85_27635"
                     /inference="COORDINATES: protein motif:HMM:NF040238.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase family protein"
                     /protein_id="HEY0137901.1"
                     /translation="IQNPGLKPNWALVIGSTYEGMGKDLMLEPVRVALGASNVREITP
                     EDLDSTYTWFLAKCRLVLVEEMKLSERKATMNRLKPLVAAPPYTLSVNAKFEPQYEIP
                     NIIAAIFFTNVENALAISSQGRRYFVTWNDGQPRDATYYEGLVAWYRGGGAALAARWL
                     LQLDVSDFKAQGRAPDSGAKDDMRRASRTLLEEWLEDSIADVAAPFDTDIVALEDVLA
                     RMPDHVTGRSRPTGQRLSATLKRLGAVYLGRVRLGKALPNMASDRAALWAIRRAGEVS
                     HLSAPTIRDRYLSQLAAVEKQVEDLFT"
BASE COUNT          274 a          578 c          581 g          260 t
ORIGIN      
        1 gacccatcgg cgaagccaag ctccgcgccg ctggcgttca ggacgaggcg tggttcgagg
       61 acctgaccat gatccccgag cgccccgagg ttttcatcat gggcagggcg cggggcgtcg
      121 aggtgttcct gtcgcaaagc ctcggcggcg ttgaggtcct gcgctgccga ccccgtggcc
      181 gcccgtccga gattgagccc ggcggacgct ggctcgctat tgaggcgtgg cggcagggcg
      241 aacggataga cctggccgag gcccggcccc acatcgccgc atcgctcgat gcggtcgcca
      301 aaggcggagt gctggcaagc gtatgatccc gaacgagtac cgacacccgc ccgcccgctg
      361 ccccaactgc ggggccaagg tggactgcgc cacgaacatg tcgcgcgaca ggggcgggcc
      421 gatggaggcg ggcgacgtga ccgtgtgcct cgactgcgcc agcatcgccg tctgcgacaa
      481 ggtcacgagg acggggcggc cggtgctgcg gaagccgacc ggcaaggaga aggcggtgtt
      541 tcggcaggac ccccgactcg ggcaggttca ggccgcgatc atcatgcaag ctcgccaatc
      601 tggccgcgag ctgcgcgaca cgccgcctgt cgtgcgccca gcgcccagcg gtacgcgcca
      661 gtgatcctaa gcgcggccac agacgccaaa aagccccgac gcctgagcgc cggggcaagg
      721 ttttgggtgg atcaggagcc tcgacgccgt aagtcgggat cgtcacgcgg ccgacgccgc
      781 ctcatgtgaa caggtcctcg acttgcttct cgaccgccgc cagctgcgac aggtagcggt
      841 cgcggatcgt aggggccgac aggtgcgaaa cctcccctgc ccgccgtatc gcccacagcg
      901 ccgcacgatc gctcgccatg ttgggcaggg ccttcccgag gcgcacgcgc ccgaggtaga
      961 ccgcgccgag gcgcttgagc gtggcggaga ggcgctggcc ggtcggcctg ctcctgccgg
     1021 tgacgtgatc cggcatccgc gccagcacgt cctccagcgc cacgatatcc gtatcgaacg
     1081 gggcggcgac gtcggcgatg ctgtcctcaa gccactcctc cagcagcgtc cgcgacgccc
     1141 ggcgcatgtc gtccttagcg cccgagtcgg gagcccggcc ctgagccttg aagtccgaca
     1201 cgtccagctg gaggagccag cgcgcggcga gggccgcacc gccgccccga taccaagcca
     1261 cgagcccctc atagtaggtc gcgtcgcgag gctggccgtc gttccacgtc acaaagtagc
     1321 ggcggccctg cgagctgatc gccagcgcgt tctcgacgtt cgtaaagaag atcgccgcga
     1381 tgatgttggg gatttcgtac tgcggctcga acttggcgtt gacgctcagg gtgtacggcg
     1441 gggccgccac gagcggcttg agccggttca tggtggcctt gcgttcgctg agcttcatct
     1501 cctccaccag gaccaaccgg cacttggcga ggaaccatgt gtaggtgctg tcgaggtcct
     1561 cgggcgtgat ttcgcggacg ttggacgccc ccagcgccac gcgaaccggc tccagcatca
     1621 aatccttgcc catgccctcg taggtcgatc cgatgaccag cgcccaattg ggtttcaggc
     1681 cggggttctg gat
//
LOCUS       DASWNJ010001666         3460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_412344, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001666 DASWNJ010000000
VERSION     DASWNJ010001666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412344"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VGB85_27640"
     CDS             <1..182
                     /locus_tag="VGB85_27640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137902.1"
                     /translation="AFSLLAMHAFDARASNHFFAEEGRLGRDVLANATERLLAAMFDT
                     APQGPDAPVEPATSP"
     gene            252..1040
                     /locus_tag="VGB85_27645"
     CDS             252..1040
                     /locus_tag="VGB85_27645"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY0137903.1"
                     /translation="MIDDLSPNYWKGRKSTECLLLHYTANESLAEAVDMMRARSTSAH
                     YIVGIDGEIRRIVREANRAWHAGQSAWNGKDQVNHFSIGIELVNFGWGETIEAGKLRR
                     TQDGGVVYANAAPAEVVDDRPASQGFVWAAYPEAQLAALLPLIKDIVKRNKIDPTQVI
                     GHEHVAPGRKRDPGPAFPWPRVATTLAGLPHSERQVARAVQSHCARLGFPPSGGVDGG
                     WGGGTDAAIARLVARHGAVYGLSAPSKSWAARRAFAEDLRRVPG"
     gene            1144..3363
                     /locus_tag="VGB85_27650"
     CDS             1144..3363
                     /locus_tag="VGB85_27650"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEY0137904.1"
                     /translation="MIAHLPRPRLALALAAPLALLAPTACGDSTAVTATEDGTAESGT
                     TAASSATPTTSAGPGPGPSTVTDPPLTTSAEGSATGTSETTDTPDPPKFCEGEPPLST
                     SGLVFDGSSSVVMGAAPELGLSTFTLEAWVRRDGRGKSAGTGVGGLKLVPIISKGRGE
                     SDGSNVDCNYNFGFYGDVLGADFEDMATGANHPIYGKTALALGTWYHVAATYDGSTWR
                     LYVDGVLDAESKADATPRHDSIQHFGLGAAFNSMGEAAGGLLGAIDEVRVYSRALDAA
                     EILATRDSTTPNPADLVAHYHLDAAEATALDSAGSLPGTIVGASFAAPGAVLDRGAAP
                     ELANPQALDAGGARTLSVDVTDAENDTVQVDFYARELTPADDFTIVALPDTQYYTRDA
                     APPTRPNPDDPEYFKAQTRWAWDHRVERNVIGLLTLGDIINNSDQPNQWKRASASLGI
                     LEDLSDPAYPEGLPYAASYGNHDQNPKDMPEATKEANSYFGVERFGKRSYYGGNFDGD
                     NDENYVYFQTGGLPVVIVNFQFNPEPDADVLAWARGVFESHPRALGIVTTHYIVTGGG
                     NFSDQGKAIYAALKDVPNVQLMASGHVAQAARRTDEFEGNVIHSMLSDYQRSAPDPDD
                     PSRPIVVAQSQTNGGQGYMRIWNFAPSRQELFVESYSPKKDAAYTDEQNEFTLAVDLL
                     GAGRSPFIPVGTTTAEGGTATVELPNFAADKTYEWYAVARDCSHARPLDLQQILPAP"
BASE COUNT          555 a         1403 c         1048 g          454 t
ORIGIN      
        1 ccgccttctc gctgctcgcc atgcacgcct tcgacgcccg cgcgagtaat catttcttcg
       61 ccgaggaagg ccgcctcggc cgcgacgtcc tcgccaacgc gaccgagcga ttactcgccg
      121 cgatgttcga cacggccccc caggggcccg acgcgcccgt cgagccggcc acatcgccct
      181 gagctcgcgc gtttttgcgg ctgccgtctc ctggcgagat acgatatcgt ggcccgctcc
      241 gggcccatcg catgatcgac gatctctcgc cgaactactg gaagggtcgc aagtcgaccg
      301 agtgtctgct gctgcattac accgccaacg agagcctcgc cgaggcggtc gacatgatgc
      361 gcgcgcgcag cacctcggcg cactacatcg tcggcatcga cggtgagatc cgacggatcg
      421 tccgcgaggc caaccgcgcc tggcacgccg gacagagcgc gtggaacggc aaggatcagg
      481 tcaaccattt ttcgatcggc atcgagctgg tcaacttcgg atggggcgaa accatcgagg
      541 ccggcaagct gcggcgcacg caggacggcg gcgtcgtgta cgccaacgcc gcccccgccg
      601 aggtcgtcga cgatcgcccc gcctcgcagg gcttcgtgtg ggctgcgtac cccgaggccc
      661 agctcgccgc cttgctcccg ctgatcaagg acatcgtcaa gcgcaacaag atcgacccga
      721 cccaggtcat cggccacgag cacgtcgcac ccggacgcaa gcgcgacccc ggccccgcct
      781 tcccgtggcc ccgcgtcgcc accaccctcg ccggcctccc acactccgag cgccaggtcg
      841 cccgcgccgt ccagagccat tgtgcacgcc tcggcttccc gccctccggc ggcgtcgacg
      901 gcggctgggg tggcggcacc gacgctgcga tcgccaggct cgtcgcgcgt cacggcgccg
      961 tgtatgggct cagcgccccc agcaagtcat gggccgcgcg gcgagcgttc gcggaggacc
     1021 tgcgtcgcgt cccgggatga tcgcgcgatc ggagcgaggc gcgccgacca caccgatgat
     1081 cgtcgatcac atcgccgcga tggagacttg tcgcgcgcga gcgccacgtc gcatcatgcc
     1141 ccggtgatcg ctcatctgcc tcgtccccgc ctcgccctcg ccctcgctgc ccccctcgcc
     1201 cttctggccc cgaccgcgtg cggcgactcg acggccgtca ccgcgaccga ggacggcacc
     1261 gccgagagcg gcaccacggc cgcctcctcc gccacgccga ccacctccgc cggtcccggc
     1321 ccgggcccgt cgaccgtcac cgacccgccg ctgaccacct ccgccgaggg ctccgccacc
     1381 ggcaccagcg agaccaccga cacccccgac ccgcccaagt tctgcgaggg cgagccgccg
     1441 ctgtcgacca gcggcctggt gttcgacggc agcagcagcg tcgtcatggg cgccgcgccg
     1501 gagctcgggc tcagcacctt cacgctcgag gcgtgggttc gtcgtgacgg tcgcggcaag
     1561 tcggcgggca cgggagtcgg cggcctcaag ctcgtcccga tcatcagcaa gggtcgcggc
     1621 gagagcgacg gcagcaacgt cgactgcaac tacaacttcg gcttctacgg cgacgtgctc
     1681 ggcgccgact tcgaggacat ggcgaccggc gccaaccacc cgatctacgg caagaccgcg
     1741 ctcgcgctcg gcacctggta tcacgtcgcc gccacctatg acggcagcac ctggcgcctc
     1801 tacgtcgacg gggtgctcga cgccgagtcc aaggccgacg ccaccccgcg ccacgactcg
     1861 atccagcact ttggcctcgg cgccgccttc aactcgatgg gcgaggccgc cggcggcctg
     1921 ctcggcgcga tcgacgaggt ccgcgtctac tcgcgtgccc tcgacgccgc cgagatcctc
     1981 gcgacccgcg acagcaccac cccgaacccg gccgacctcg tcgcgcacta tcacctcgac
     2041 gccgccgagg cgaccgcgct cgacagcgcc ggaagtctcc ccggcacgat cgtcggggcc
     2101 agcttcgcgg cccccggcgc cgtcctcgat cgcggcgccg cgcccgagct cgccaacccc
     2161 caggcgctcg acgctggcgg cgcgcggacc ctctccgtcg acgtcacgga cgccgagaat
     2221 gacaccgtgc aggtcgactt ctacgcgcgc gagctgaccc ccgccgacga cttcacgatc
     2281 gtcgcgctgc ccgacaccca gtactacacg cgcgacgccg ccccgccgac ccgcccgaac
     2341 cccgacgacc ccgagtactt caaggcgcag acccgctggg cctgggacca ccgcgtcgag
     2401 cgcaacgtca tcggcctgct gaccctcggc gacatcatca acaactccga ccagccgaac
     2461 cagtggaagc gcgccagcgc ctcgctcggc atcctcgagg acctcagcga ccccgcctac
     2521 cccgagggtc tgccgtacgc cgcctcctac ggcaaccacg accagaaccc caaggacatg
     2581 cccgaggcga ccaaggaggc caacagttac ttcggcgtcg agcgcttcgg caagcgctcc
     2641 tactacggcg gcaacttcga cggcgacaac gacgagaact acgtctactt ccagaccggc
     2701 ggcctgccgg tcgtcatcgt caacttccag ttcaaccccg agcccgacgc cgacgtgctc
     2761 gcgtgggcgc gcggcgtctt cgagtcgcac ccgcgcgcgc tcggcatcgt caccacccac
     2821 tacatcgtca ccggcggcgg caacttcagc gaccagggca aggcgatcta cgccgcgctc
     2881 aaggacgtgc ccaacgtgca gctcatggcc tcgggtcacg tcgcccaggc cgcgcgccgc
     2941 accgacgagt tcgagggcaa tgtcatccac tcgatgctct ccgactacca gcgcagcgcg
     3001 cccgaccccg acgacccctc gcgcccgatc gtcgtcgccc agtcgcagac caacggcggc
     3061 cagggctaca tgcggatctg gaacttcgcg ccgtcgcgcc aggagctgtt cgtcgagagc
     3121 tacagcccca aaaaggacgc cgcctacacc gacgagcaga acgagttcac cctcgcggtc
     3181 gacctcctcg gcgccggccg cagcccgttc atcccggtcg gcaccaccac cgccgagggc
     3241 ggcaccgcga cggtcgagct cccgaacttc gccgccgaca agacctacga gtggtacgcc
     3301 gtcgcccgcg actgctcgca cgcccgcccg ctcgacctcc agcagatcct ccccgctccc
     3361 tgacgatgaa gcccggcccg cgcgacctga tcctctggga catcctcgag gagcacgccg
     3421 acgaggccgg gttcctcggc gcccagtggg agcgcgccct
//
LOCUS       DASWNJ010001667         4047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_625763, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001667 DASWNJ010000000
VERSION     DASWNJ010001667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625763"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..876
                     /locus_tag="VGB85_27655"
     CDS             253..876
                     /locus_tag="VGB85_27655"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY0137905.1"
                     /translation="MYTLHYSPGSASMLIHLLLLEIGAAHELRRVDLEAGAQRDPAYL
                     RLNPGGVVPTLIVDGQPCWETAGLALLLSERHPEAGLAPLPGSPLRPAHLQWVAYLAN
                     ALQPAFRTWFYPEAAEPEAGVIMAAARRRIEACWDRIDAHLAGGPYLLGDQPSVADFY
                     ATMLMRWSRKMPRPATDWPQLAALAGRIKARPSWQRLYEIEDLTEWA"
     gene            935..1372
                     /locus_tag="VGB85_27660"
     CDS             935..1372
                     /locus_tag="VGB85_27660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137906.1"
                     /translation="MTIAIVGLLGQGSYVLLTHLRTRIGSYTSPDVSSLLRLGSIPLI
                     VAVAIAVYFLGPRLEAARSLKDSLYWGVYICFAISFISASLYLALKTAFAEPSGVPSG
                     LATHHWVIIVFVQMLIYCFISAMIFGPILGYLSFDRPIAGALR"
     gene            1691..2824
                     /gene="thiH"
                     /locus_tag="VGB85_27665"
     CDS             1691..2824
                     /gene="thiH"
                     /locus_tag="VGB85_27665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HEY0137907.1"
                     /translation="MPTFSTVYSTLPIADLVRRSLDTTATDLAPIVRRGHAESLSEFA
                     ALLSPAAAADLPALTGLSQALAVRHFGKAIRLFAPLYLSNECVNVCTYCGFSRTNDIP
                     RITITVERAVQEVTLLRARGIASLLLVAGEHPKFVSDGYVEAVIRALLPVMPEVSIEI
                     GPLETPAYVPIVEAGCAGLVVYQETYHEPTYRAVHTAGPKKRYHWRLDAPERGHAAGF
                     RRLGIGALYGLHDFRHEALSLAAHADHLRRACPGVQLAISVPRLRPAAGEFQPDPRFM
                     IGDPEFLQLICALRMFIPDADIIVSTRESADLRDDMVSIGVTTMSAGASTEPGGYSDF
                     DQDAWTPARAQPGEQFHIADERPPQAVAEMIRRRGYLPIWQGA"
     gene            complement(2871..3455)
                     /locus_tag="VGB85_27670"
     CDS             complement(2871..3455)
                     /locus_tag="VGB85_27670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137908.1"
                     /translation="MRFLPLLVLLLPGCQTPEAAPDPKPVHVPVTPGPAPVIPLAPKP
                     TPVAESPAPVPLEAPPTASVFVAPALEQGKGRTAKYVDAPKYDANNVADMVPGASSPE
                     AAVVHYLASRIRGDEHYREVMLARCSRGCGLAYHDRWKFRAFRLVSRTQVATGKFEVK
                     AWFEVKAQGSVESGEDEFTVVAEGDEFRIAEVPS"
     gene            complement(3644..>4047)
                     /locus_tag="VGB85_27675"
     CDS             complement(3644..>4047)
                     /locus_tag="VGB85_27675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137909.1"
                     /translation="MVHMQVTEFPTLEIQNNAFALRDADERLLAAGLKNAIFPQPAPL
                     EVAPLTFDLLATDCEPLDDGYVVMQRAGLSVTMDGMTAVVFDGNTANLGLQDGFQILV
                     SRATRELCVANNRFNGEPWGVDALLVFTPEG"
BASE COUNT          582 a         1506 c         1326 g          633 t
ORIGIN      
        1 gggcgaagcc aaggcgaagc agcatcgacg cggcacgacc atatcacgcg gggccgccga
       61 catggatgcc gcgcgtgcgc tcgtcttcgc gggtggggcg aggccgagcc ggcggcccgc
      121 gccgacccta gccatcaagg acgccgtggg catcggcgag gagatcgcgg atgtcctgcc
      181 agcgccatgc agcgccgtga ccgctcgcct tttcgcgcag cgagcggcta tcgtgtcggt
      241 cgaggcgcct gcatgtacac gctccactac tcgcccgggt ccgccagcat gctgatccat
      301 ctgctcctgc tcgagatcgg tgcggcgcac gagctgcggc gcgtcgacct cgaggcgggg
      361 gcccagcgcg accccgcata tcttcggctc aacccggggg gcgtggtacc gacgctgatc
      421 gtcgacgggc agccgtgctg ggagaccgcc gggctcgcgc tgctgctgag cgagcgccac
      481 cccgaggccg gcctcgcccc gctgccggga tcaccgctgc ggcccgccca cctgcagtgg
      541 gtcgcctacc tcgccaacgc gctgcagccg gcgtttcgca cgtggttcta tcccgaggcc
      601 gcggagcccg aggcgggcgt gatcatggcg gccgcgcgcc ggcgcatcga ggcgtgctgg
      661 gaccgcatcg acgctcacct cgccggcggt ccctacctgc tcggcgacca gccgagcgtc
      721 gccgactttt atgcgacgat gctgatgcgc tggtcgcgaa agatgccgcg cccggccacc
      781 gactggccgc agctggccgc gctcgccggc cgcatcaagg cgcgaccgag ctggcagcgc
      841 ctgtatgaga tcgaggacct gaccgagtgg gcatgaccgg atgacgcggg tgtcgttcgc
      901 agcaggctcg cccgaaggac acatgcgaac agcgatgacg atcgcaattg tcgggctcct
      961 gggccagggg agctacgtgc tgctcacgca cctcaggacg aggatcggca gctatacctc
     1021 accggacgtg tcgagcctgc ttcgactcgg ctccatccca ctaatcgtcg ccgtggcgat
     1081 cgccgtctac tttttgggcc cacggctcga agccgcccgg tcactgaaag attcgcttta
     1141 ttggggcgtt tatatttgct ttgcgatcag ctttatctcc gcgagccttt atctcgcgct
     1201 gaaaactgcc ttcgcggagc cgagcggcgt gcccagcggc ctggcgacgc atcattgggt
     1261 gatcatcgtc ttcgtccaga tgttgatcta ttgcttcatc agcgcgatga tcttcgggcc
     1321 gatcctgggc tacctgagct tcgatcgtcc catcgcgggc gctctgcgct agaacgagcg
     1381 gcgacgcgtg cacggcagcg gcagcccgag atcgcgggcg acgaggacgc ggccggcgac
     1441 gggcgcagcc tcacggacga caccaggccg gctcgctccc tgctcgcccg ggcgctgctg
     1501 ctgcgcagac gcggtcgccc ggcgtcgagg ctcacccgcg atcgccgcga gtgagcctgc
     1561 cggcgacact ctggccgccc gcaccgcttg ctgccgcggt cccgccgggc catggtccgc
     1621 ccgaccgtgt cgcaccagca cgcgacgctg cgccgggatg cggtagtcgt ctcaccgata
     1681 caccataccc atgccgacat tttcgaccgt ctactccacc ctcccgatcg ccgacctcgt
     1741 tcgtcgctcg ctcgacacca ccgcgacgga cctggcgccg atcgtccgcc gcggccacgc
     1801 ggagagcctc tccgagttcg cggcgctgct gtccccggct gcggccgccg atctgccggc
     1861 cctgaccggg ctctcgcagg cgctcgcggt gcgccacttc ggcaaggcga tccgcctgtt
     1921 cgcgccgctg tacctgtcca acgagtgcgt caatgtctgc acatattgcg ggttctcgcg
     1981 caccaacgac atcccgcgga tcacgatcac ggtcgaacgc gcggtgcagg aggtcacgct
     2041 gctgcgcgcg cgtggcatcg cctccctgct gctcgtcgcc ggcgagcacc cgaaattcgt
     2101 gtccgacggc tacgtcgagg cggtgatccg cgcgctgctg ccggtgatgc ccgaggtctc
     2161 gatcgagatc gggccgctcg agacgccggc ctacgtgccg atcgtcgagg cgggctgcgc
     2221 cgggctcgtc gtctatcagg agacctacca cgagccgacc taccgcgcgg ttcatacggc
     2281 ggggcccaag aagcgctatc actggcggct cgacgccccg gagcgcggcc acgccgcggg
     2341 cttccggcgg ctcggcatcg gcgcgctgta cggcctgcac gacttccgcc acgaggcgct
     2401 ctcgctcgcg gcccacgccg accacctgcg tcgggcctgc ccgggcgtgc agctggcgat
     2461 cagcgtgccg cgcctgcgcc ccgccgccgg cgagttccag cccgacccgc gcttcatgat
     2521 cggcgacccc gagttcctgc agctgatctg cgcgctgcgc atgttcatac ccgacgccga
     2581 catcatcgtc tccacccgcg agtccgccga cctgcgcgac gacatggtgt cgatcggcgt
     2641 caccaccatg agcgccggcg cgagcaccga gcccggcggt tacagcgact tcgatcagga
     2701 cgcctggacc cccgcccgcg cgcagcccgg cgagcaattc cacatcgccg acgagcggcc
     2761 gccgcaggcg gtcgccgaga tgatccgccg ccgcggttac ctgccgatct ggcaaggcgc
     2821 ctgacggcga gcctcccccg ctgcttcgcg tgccgccgtg atgctacgcg tcaactcggc
     2881 acctcggcga tgcgaaactc gtcgccctcc gcgaccaccg tgaactcgtc ctcgccggac
     2941 tccacgctgc cctgggcctt cacctcgaac catgccttca cctcgaactt gccggtcgcc
     3001 acctgcgtcc gcgacaccag gcggaacgcc cggaacttcc accggtcgtg gtacgcgagc
     3061 ccgcacccgc gcgagcaccg ggccagcatg acctcgcggt agtgttcgtc tccccggatt
     3121 cggctcgcca ggtaatgcac gaccgcggcc tccggcgagc tcgcgcccgg caccatatct
     3181 gcgacgttgt ttgcgtcgta cttcggcgcg tcgacatact tcgccgtccg ccccttcccc
     3241 tgctcgagcg ccggcgcgac gaacacgctc gccgtcgggg gcgcttccag gggcaccggt
     3301 gcgggtgact cggcgacggg cgtcggcttg ggtgccagcg ggatgacggg tgccggaccc
     3361 ggcgtcaccg gcacatggac gggcttcggg tcgggcgcag cctcgggggt ctgacagcct
     3421 ggcagcaaca acacgagcaa cggcaggaag cgcatccccg acaacgacca tggccgccgc
     3481 ccgaagttcc gcctcacgac gccgacgacg gcgcgcatac gcgtggcgcc ggctgcaacc
     3541 ccgggggcgg ccgacggatc cgcccatcct cgactccccg gaccggacct cgatcgatct
     3601 cgtcgtcccc tgagccgcga gagcgagcga cgcgctgcct ggctcagccc tccggtgtga
     3661 acacgagcag cgcgtccacc ccccacggct caccattgaa ccggttgttg gcgacgcaca
     3721 gctcccgtgt cgcgcggctg accaggatct gaaagccgtc ctgcaggccg aggttggcgg
     3781 tgttgccgtc gaacaccacg gcggtcatcc cgtccatcgt gaccgacagt ccggcgcgct
     3841 gcatcacgac gtagccgtcg tcgagcggct cacagtcggt tgcgagcaga tcgaaggtca
     3901 ggggggcgac ctccagcggc gccggctgcg ggaagatcgc gttcttcagg cccgccgcga
     3961 gcagccgctc gtccgcgtcg cgcagcgcga aggcgttatt ttggatctcc agggtcggga
     4021 attccgtcac ttgcatgtgc accatcg
//
LOCUS       DASWNJ010001668         1773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_699626, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001668 DASWNJ010000000
VERSION     DASWNJ010001668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699626"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /locus_tag="VGB85_27680"
     CDS             <1..1252
                     /locus_tag="VGB85_27680"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEY0137910.1"
                     /translation="QKYGGSSVASAAHMKRVAERIVAAKKNGDDVVVVVSAMGDSTDE
                     LLDLAGQITDDPPGRELDMLLTAGERISMALLAIAISTHGYEARSFTGSQAGVITTGS
                     HGRARIIDVTPGRLRSALDEGSIVIVAGFQGVSQDTKDITTLGRGGSDTTAVAVAAAL
                     DADVCEIYTDVDGVFTADPRIVPNAKRLDTITYEEMLELAASGAKVLMLRCVEYARRY
                     GIPVHVRSSYSQLPGTIVSGSMEDLPVEQAIITGVAHDRSEGKITVYGVPDRPGEAAQ
                     LFRVLADAEINIDMIVQNVSAEASKLADISFTLPKSDGPAALAALERVKHTVGYTDVS
                     FDQHIGKVSLVGAGMRSHPGVSAKFFGALADAGVNLELISTSEIRISVVCRDTDVDIA
                     VRAVHDAFDLGSDVEAVVYGGTGR"
     gene            1249..>1773
                     /locus_tag="VGB85_27685"
     CDS             1249..>1773
                     /locus_tag="VGB85_27685"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY0137911.1"
                     /translation="MSSFSDSGLRVGIVGATGQVGRVVRDILAERDFPLAELRFFASA
                     RSAGSTLPWDGGEITVEDAATADPTGLDVALFSAGATTSRAQAPRFAEAGVIVIDNSS
                     AFRRDPEIPLVCSEVNPGDIAKATGGIIANPNCTTMAAMPVLKPLHDEAGLVRIVAST
                     YQAVSGSGIAGVQEL"
BASE COUNT          272 a          670 c          578 g          253 t
ORIGIN      
        1 ccagaagtac ggcggttcct ccgtcgcctc cgcagcgcac atgaagcgtg tcgccgagcg
       61 gatcgtcgcc gcgaagaaga acggcgacga cgtcgtcgtg gtcgtctccg cgatgggtga
      121 cagcaccgac gaactgctcg acctggccgg tcagatcacc gacgacccgc ccggccgcga
      181 gctcgacatg ctcctgaccg cgggcgagcg gatctcgatg gcgctgctgg ccatcgccat
      241 ctccacgcac ggctacgagg cgcgctcctt caccggatcg caggccggcg tcatcaccac
      301 cggcagccac ggcagggccc gcatcatcga cgtcacgccc gggcgcctgc gctcggcgct
      361 ggacgagggt tcgatcgtga tcgtcgccgg cttccagggc gtcagccagg acaccaagga
      421 catcaccacg ctgggccgcg ggggttcgga caccaccgcc gtggccgtcg ccgcggcgct
      481 ggacgccgac gtctgcgaga tctacaccga cgtcgacggt gtcttcaccg ccgacccgcg
      541 gatcgtcccg aacgccaagc ggctcgacac catcacctac gaggagatgc tcgagctggc
      601 cgcgtccggt gcgaaggtgc tcatgctccg ctgcgtcgag tacgcccgcc gctacggcat
      661 ccccgtgcac gtccgcagct cctactcaca gctccccggc acgatcgtgt ccggatcgat
      721 ggaggacctc ccggtggaac aggcgatcat cacgggcgtc gcgcacgacc gctccgaggg
      781 caagatcacc gtctacgggg tcccggaccg gccgggcgag gccgcgcagc tcttccgcgt
      841 cctggccgac gccgagatca acatcgacat gatcgtgcag aacgtgtcgg ccgaggccag
      901 caagctcgcc gacatctcct tcacgctgcc caagagcgac ggcccggcgg ccttggccgc
      961 cctggaacgt gtgaagcaca ccgtgggtta caccgacgtg agcttcgacc agcacatcgg
     1021 caaggtgtcg ctggtcggcg ccggcatgcg gtcgcacccg ggcgtctcgg ccaagttctt
     1081 cggcgccctg gccgacgccg gcgtgaacct cgagctcatc agcacctcgg agatccgcat
     1141 ctccgtggtc tgccgcgaca ccgacgtcga catcgccgtc cgcgcggtgc acgacgcgtt
     1201 cgacctcggc tccgacgtcg aggccgtcgt ctacggaggg accgggcgat gagctcgttc
     1261 agtgacagcg gcctgcgggt cggcatcgtc ggcgcgaccg gtcaggtcgg ccgcgtcgtc
     1321 cgcgacatcc tggccgagcg ggacttcccg ctcgccgagc tgcgcttctt cgcctccgca
     1381 cgctcggccg gcagcacgct gccctgggac ggtggcgaga tcaccgtcga ggacgccgcc
     1441 accgccgacc ccacgggtct ggacgtggcg ctgttctccg cgggcgccac cacctcccgc
     1501 gcgcaggccc cccggttcgc cgaggccggc gtcatcgtga tcgacaacag ctccgctttc
     1561 cggcgcgacc cggagatccc gctggtctgc agcgaggtca accccggcga catcgccaag
     1621 gccaccggcg gcatcatcgc caacccgaac tgcaccacca tggccgcgat gccggtgctc
     1681 aagccgctgc acgacgaggc cggcctggtc cgcatcgtcg ccagcaccta ccaggccgtg
     1741 tcgggctccg gcatcgccgg cgtgcaggag ctg
//
LOCUS       DASWNJ010001669         4901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_381401, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001669 DASWNJ010000000
VERSION     DASWNJ010001669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381401"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VGB85_27690"
     CDS             complement(<1..414)
                     /locus_tag="VGB85_27690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137912.1"
                     /translation="MSAAVITAPIALADWLRRRWLRVLGAYARPIVRDREVRVALMFS
                     AVIVTALAGAVLVPMWLLILGPLVWGVPHLVADLRYLVVRTGYHRRPLLGLVAGLPLL
                     WLALGGELVWGFVGACGAALVARARLMPRLLAVAVL"
     gene            complement(544..1743)
                     /locus_tag="VGB85_27695"
     CDS             complement(544..1743)
                     /locus_tag="VGB85_27695"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0137913.1"
                     /translation="MHTGSPRATQAWTRMATSFANDATLAESSQAAASLVASGPRPRS
                     ISVLPTVRSEGGQIQLTHESGDRYRSLGVLGTGGMGVVERAEDIDIGRPVAIKRLLPE
                     ASNAIGVARFVSEIRIVGSLDHPNVVPIHDVGVDADGRYFFVMKYVEGQTLADIIERL
                     SAGDPAALAFWTSERRVELMIAVLQALDYAHERGVLHRDVKPENIMVGTHGEVRLMDW
                     GVARRIGVADPGEDVTPGLIGTPAYMSPEQAALKDLDARSDIYAAAVVLHEFFALHHY
                     LAHHADSLYGLLEAVQTETVDCMDSGRDSPKGRLPPELRHICWRGMQKDPANRYQSAK
                     ELIDDLRRYIDGTYEVKCMFTFTKRALREGGRFVDRRPNAAVALAATHTAFSLFGVGA
                     LIYMLIA"
     gene            complement(1779..2864)
                     /locus_tag="VGB85_27700"
     CDS             complement(1779..2864)
                     /locus_tag="VGB85_27700"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0137914.1"
                     /translation="MSSADLDVAIVGAGQAALALAHGLRGSGLDYACLGADARIGDSW
                     RRRWDSLRLFTPAQYDGLPGLPFPAPHDHYPGKDEVADYLEAYSSHFALPVRLDTRVT
                     RTERDEHSFILSTGHDEIRAQQVVLATGSYGAPWTPAFADRLAPAIVQLHAAHYRRPD
                     ALPGGRVTIVGSGASGLQIAAELAATHSVTLCIGRRQPRLPQRLLGRDIWFWLERTPN
                     VPADTWLGRHLARKEPVVGEGPRALARRGVKLAARATGADGSALHLADGTRLPVTTVV
                     WATGHRHALDWVDLPVRDPHGAPLHHDGRSPVPGLYFLGLRWMRSRGSSLLGWVGEDA
                     AYIAGQLRMRALARPMRRGTSDGRGTE"
     gene            complement(2861..3517)
                     /locus_tag="VGB85_27705"
     CDS             complement(2861..3517)
                     /locus_tag="VGB85_27705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0137915.1"
                     /translation="MPTWREAEVIADTVAACNAVADETIVADAGSPDDTATLAAAAGA
                     RVIAAPRGRGPQQNAGARAAIGEVLLFVHADTHVPVQARAAIASALADPTILGGNFKL
                     RFAPATRAARIYGLANHIRRRALRMYYGDSCIFVRRAVFDRLGGFPDLPIFEDHAFVR
                     RLEATGRTHYETSVLVTTSSRRFLNSPIRTLGTWALLQGLYTCGVPAGRLARLYRDIR
                     "
     gene            complement(3541..4578)
                     /gene="arsS"
                     /locus_tag="VGB85_27710"
     CDS             complement(3541..4578)
                     /gene="arsS"
                     /locus_tag="VGB85_27710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255296106.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HEY0137916.1"
                     /translation="MTATKLRPSLLRTGHDLASAANQLRILGDLPLPDFAAHLAAAGL
                     APLRARAIEILQVNLGKRCNQTCQHCHVDAGPDRREVMPDAVVDACLDFLSRHDIPTL
                     DITGGAPELHPRFRELVTRARALGRRVIDRCNLTITRLPNYADLPAFFAAHHVEVVAS
                     LPWYRATQTDAQRGDGVFEDSIAALRHLNGLGYGMPDSGLVLNLVTNPAGAFLPAGQA
                     ALERDWKRELQRLHGVHFNNLYTITNMPISRYLEYLERNHQLATYMQKLLAAFNPRAV
                     AGVMCRDTLSVGWDGRLYDCDFNQMLDLALPGTILDADLATLATREIRTAAHCYGCTA
                     GAGSSCGGATT"
     gene            complement(4575..>4901)
                     /locus_tag="VGB85_27715"
     CDS             complement(4575..>4901)
                     /locus_tag="VGB85_27715"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HEY0137917.1"
                     /translation="EYYKPEHLARFAEIGKDAPELWAKFLEWYGAVFAEGALSEREKA
                     LIALAVAHSVQCAYCIDAYSTTCLEKGADRQQMTEAVHVAAAIRGGSSLIHGLQMRDH
                     VHKVSM"
BASE COUNT          730 a         1538 c         1966 g          667 t
ORIGIN      
        1 gagcacggcg accgcgagca gacggggcat gaggcgagcc cgggcgacca gcgcggcccc
       61 gcaggcgccg acgaagcccc acacgagctc accgccgagc gcgagccaca gcagcgggag
      121 accggcgacg aggccgagca atgggcgccg gtggtagccg gtgcggacga cgagatagcg
      181 caggtcggcg accaggtgcg gcacgcccca cacgagtggg ccgaggatca gcagccacat
      241 cgggacgagc acggcgccgg ccagcgcggt gacgatgacg gcggagaaca tcagcgcgac
      301 gcgaacttcg cggtcacgga cgatcggccg cgcatatgct ccgagcacgc gcaaccaccg
      361 acgtcgcagc caatcggcga gggcgatcgg cgcggtgatg acggcagcgc tcacggggcg
      421 aggcgctccc cggacacgag ctcgcggctg cgatccacac cgaccatcac gtacgaccgc
      481 agcttcgcat gcatcgcggg cggcgtacac cgcccgtgat cggacaccgg cgagcgatcg
      541 tgctcaggcg atcagcatgt agatcagcgc gccgacgccg aacagcgaga aggcggtgtg
      601 ggtggccgcg agcgcgacgg cggcgttggg gcgacggtcg acgaagcggc cgccctcgcg
      661 cagcgccctc ttggtgaagg tgaacatgca cttgacctcg taggtgccgt cgatatagcg
      721 gcgcaggtcg tcgatcagct ccttggcgct ctggtagcgg ttcgcggggt ccttttgcat
      781 gccgcgccag cagatgtggc gcagctcggg cggcaggcga cccttcgggc tgtcgcgccc
      841 cgagtccatg caatccaccg tctcggtctg cacggcctcg agcaggccgt acagcgagtc
      901 ggcgtggtgg gcgaggtagt ggtgcagggc gaagaactcg tgcagcacga cggcggcggc
      961 gtagatgtcg ctgcgcgcgt cgaggtcctt gagcgcggcc tgctcgggcg acatgtacgc
     1021 gggggtgccg atcaggccgg gggtgacatc ctcgcccggg tcggcgacgc cgatccgccg
     1081 cgcgacgccc cagtccatca agcgcacctc gccgtgggtg ccgaccatga tgttctcggg
     1141 cttgacgtcg cggtgcagga cgccgcgctc gtgggcgtaa tcgagggcct gcaggacggc
     1201 gatcatcagc tcgacgcggc gctcggacgt ccagaaggcg agcgcggccg ggtcgccagc
     1261 ggacaggcgc tcgatgatgt cggcgagggt ctggccctcg acatacttca tgacgaagaa
     1321 gtagcggccg tcggcgtcga cgccgacgtc gtggatcggg acgacgttgg ggtgatcgag
     1381 gctgccgacg atgcggatct cgctgacgaa gcgcgcgacg ccgatcgcgt tgctcgcctc
     1441 cggcagcagc cgcttgatcg cgaccggacg gccgatgtcg atgtcctcgg cgcgctcgac
     1501 cacgcccatg ccgccggtgc cgagcacccc gaggctgcgg tagcggtcgc ccgactcgtg
     1561 cgtgagctgg atctggccgc cctcgctgcg cacggtcgga agcacggaga tcgatcgcgg
     1621 gcgcgggccg gaggccacca ggctggccgc cgcctggctg gactcggcga gcgtggcgtc
     1681 gttcgcaaag ctggtcgcca tacgcgtcca ggcctgggtg gctcggggag atccggtgtg
     1741 catcgcggcg aaggctagat cgcggcgggc tcaatctgtc attcggtacc gcgaccatct
     1801 gacgtgccgc gccgcatggg gcgcgcgagc gcgcgcatgc gcagctggcc agcgatgtac
     1861 gcggcatcct caccgaccca gccgagcagc gaggagccgc gcgaccgcat ccagcgcagg
     1921 cccaggaagt acaggccggg caccggggag cggccatcgt gatgcagcgg cgcgccgtga
     1981 gggtcgcgga cggggaggtc gacccagtcg agcgcgtggc ggtggccggt cgcccagacg
     2041 acggtcgtga ccgggaggcg ggtgccgtcc gcgaggtgga gcgcggaccc gtcggcgccg
     2101 gtggcccgcg cggcgagctt caccccgcgg cgggcgagcg cgcggggacc ctcgccgacg
     2161 accggctcct tgcgcgcgag gtgacgcccg agccaggtgt cggcgggcac gttcggggtg
     2221 cgttcgagcc agaaccagat gtcgcgcccg agcaggcgct gcggcagtcg cggctgcctg
     2281 cgaccgatgc acagggtgac gctgtgcgtg gcggccagct cggcggcgat ctgcaggccg
     2341 gacgcgccgc tgccgacgat cgtcactcgc ccccccggca gcgcgtcggg gcggcggtag
     2401 tgggcggcgt gcagctgcac gatcgccggc gcgaggcgat cggcgaacgc gggggtccac
     2461 ggggcgccgt aggatccggt ggcgagcacg acctgttgcg cgcgtatttc atcgtgccct
     2521 gtcgagagga tgaacgaatg ctcgtcgcgc tcggtccggg tgacgcgggt gtcgaggcgg
     2581 accggcaggg cgaagtgtga tgaataggcc tcgaggtaat cggcgacctc gtccttgccg
     2641 ggataatgat cgtggggcgc ggggaagggc aggccgggca ggccgtcgta ttgcgcgggg
     2701 gtgaacaggc gcagcgagtc ccagcggcgc cgccagctgt cgccgatccg cgcgtcggcg
     2761 ccgaggcacg cgtaatccag gcccgagccg cgcagcccgt gggcgagcgc gagcgcggcc
     2821 tggccggcgc cgacgatcgc gacatcgaga tccgcgctac tcaacgaatg tcccggtaga
     2881 gccgcgcgag gcgcccggca ggcacaccgc aggtatagag cccctgcagc agagcccagg
     2941 tgccgagcgt gcggatcggg ctatttaaga agcggcggct gctggtggtc acgaggacgg
     3001 atgtttcgta gtgagtgcgg ccggtggcct cgaggcggcg cacgaaggcg tggtcctcga
     3061 agatggggag gtcagggaag ccgccgaggc ggtcgaatac ggcgcggcgc acgaagatgc
     3121 aggaatctcc gtagtacatt cgcagggcgc ggcggcggat gtgattggcc aggccgtaga
     3181 tccgggcggc gcgggtggcc ggggcgaagc gcaatttaaa attgccgccg aggatggtgg
     3241 ggtcggccag ggccgaggcg atcgcggcgc gggcctgcac cggcacgtgg gtgtcggcgt
     3301 ggacgaacaa caggacctcg ccgatcgccg cgcgggcgcc cgcgttttgc tgcgggccgc
     3361 ggccgcgcgg ggccgcgatc acgcgcgcgc ccgcggcggc ggcgagcgtg gcggtgtcgt
     3421 cggggctgcc ggcgtcggcg acgatcgtct cgtcggcgac ggcgttgcag gcggcgacgg
     3481 tgtcggcgat gacctccgcc tcacgccagg tcggcacgat cgcggacagc cagggccggt
     3541 tcacgtcgtc gcgcccccgc agctgctgcc ggccccggcg gtgcagccgt agcagtgcgc
     3601 ggcggtccgg atctcgcggg tggcgagggt ggcgaggtcg gcgtcgagga tcgtgcccgg
     3661 cagggccaga tcgagcattt gattaaaatc gcagtcgtag agccggccgt cccagcctac
     3721 cgagagggtg tcgcggcaca tcaccccggc gacggcgcgg gggttgaatg cggcgaggag
     3781 cttttgcatg tatgttgcga gctggtgatt gcgctcgagg tactcgaggt accgcgagat
     3841 cggcatgttg gtgatcgtgt agagattatt gaagtggacg ccgtgcaggc gctggagctc
     3901 gcgcttccag tcgcgctcga gcgcggcctg gccggccggc aagaatgcgc cggcggggtt
     3961 ggtcacgagg ttgagcacga ggccgctgtc gggcatgccg tagccgaggc cgttgaggtg
     4021 gcgcagggcg gcgatcgagt cctcgaacac gccgtcgccg cgctgggcgt cggtctgggt
     4081 ggcgcgatac cagggcaggg aggcgacgac ctcgacgtgg tgcgcggcga agaaggccgg
     4141 gaggtcggcg tagttgggca ggcgcgtgat cgtgaggttg cagcggtcga tcacgcggcg
     4201 gccgagcgcg cgggcgcggg tgacgagctc gcgaaagcgt gggtgcagct cgggggcgcc
     4261 gccggtgatg tcgagcgtcg ggatgtcgtg gcgcgacagg aagtcgaggc aggcgtcgac
     4321 gacggcgtcg ggcatgacct cgcggcggtc ggggccggcg tcgacgtggc aatgctggca
     4381 ggtctggttg caacgcttgc cgaggttgac ctgcaagatc tcgatcgcgc gggcgcgcag
     4441 cggggcgagg ccggcggcgg cgaggtgcgc ggcgaagtcg ggcagcggca ggtcgccgag
     4501 gatccgcagc tggttggcgg cggaggcgag gtcgtggccg gtgcgcagca gcgagggccg
     4561 gagcttcgtc gctgtcacat cgacaccttg tggacgtggt cgcgcatctg caggccgtgg
     4621 atcagcgagg agccgccgcg gatcgcggcg gcgacgtgca cggcctcggt catctgctgg
     4681 cggtcggcgc ctttctcgag gcaggtcgtg ctgtaggcgt cgatgcaata ggcgcactgc
     4741 acggagtggg ccacggcgag cgcgatcagg gccttctcgc gctcggacag ggcgccctcg
     4801 gcgaagacgg ccccgtacca ctcgaggaac ttggcccaca gctccggggc gtccttgccg
     4861 atctcggcga agcgggcgag gtgttcgggc ttgtagtatt c
//
LOCUS       DASWNJ010001670         2503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_65621, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001670 DASWNJ010000000
VERSION     DASWNJ010001670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65621"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2503
                     /locus_tag="VGB85_27720"
     CDS             <1..>2503
                     /locus_tag="VGB85_27720"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEY0137918.1"
                     /translation="ELSVNARPGALAQGDCASPKTARLTPICKPDDLACVRWQEDEIT
                     RLRRFMFDKGYLRGEAALALVCGRRSDEVDLHIYLAKGTGYRVPWRQLTVHGNMPPAD
                     HRWIRRTFWPRIKATPFPSRLTREAIEEAKERAERRYAEPRDNIIQATAAPELSLPYP
                     QIQIDTNYEEMRAGEVPPGGKLPLEVRVDLGRGVKTSFLGNRSYSDLRLRGQMQLFKR
                     REAPSDQAAVREAANLRAYYQSKGFLLASVRGRYEEFGNKAPGALYFQIKEGPTVKIR
                     GLEQRITRGVPPKVADDISRDFDRERKIKRGGSFTEADMIDDLGALLQRYQDRGYLCA
                     RARFSVAFWPEGLDRPGEHAEVDVATILKREPSAAWATRDFDPAGLRQLLARKRANLY
                     VRITIEPGPRVFTSRRTELVRYLDVPIPGDRNVADLPVHTDGAWGARRIFFETPLRRS
                     GDSRPGGVAVSPSLDREVEEVVVDRYRDSGFPVADAEIRWLYTNPATNAVIAVPTSRR
                     LADEDIGMCEAYTSGTAVEVETELSVYEGKKGLFGDTLLRGNFKTRGWTLRRQFAFRT
                     GDAYSREATDSTRSNIDGLGVAESVDITPYPVGCELDSEGDCVVHHVVEMREAKDYAA
                     TLSGGFGLATLDPFYVFLQPAFPNIFGTAWDFEVLGHYGFNTSNVPAGVPFLGDCAGQ
                     RCYERSARASLTRQRIFATPLTFSVGGTYQVRLTPARGRIESAVGTITFTYPITTKLQ
                     TYFGYLIQRSNISQDVVKPAFDPSVPTDGDANIVNRRDAIVSDRTGALQLGMTYTNAD
                     SPFNPEKGFIAGADLKLASPYLGGIDWFIRADLSW"
BASE COUNT          406 a         1003 c          766 g          328 t
ORIGIN      
        1 gcgagctgtc ggtcaacgcc cgccccggcg cgctcgccca gggcgactgc gcctcgccaa
       61 agacggcccg cctcaccccg atctgcaagc ccgacgacct cgcctgcgtg cgctggcaag
      121 aggacgagat cacgcggctg aggcgcttca tgttcgacaa gggctacctg cgcggcgagg
      181 ctgcgctcgc gctggtctgc ggccgtcgca gcgacgaggt cgacctccac atctacctcg
      241 ccaagggcac cggataccgc gtcccgtggc gccagctgac cgtgcacggc aacatgccgc
      301 ccgccgatca ccgctggatc cggcgcacct tctggccgcg gatcaaggcg accccgttcc
      361 cctcgcgcct gacccgcgag gcgatcgagg aggccaagga gcgggccgag cgacgctacg
      421 ccgagccgcg cgacaacatc atccaggcga ccgccgcccc cgagctcagc ctcccctacc
      481 cgcagatcca gatcgacacc aactacgagg agatgcgcgc cggcgaggtg ccgcccggcg
      541 gcaagctgcc gctcgaggtg cgcgtcgacc tcggccgagg cgtcaagacc tcgttcctcg
      601 gcaaccgcag ctactccgat ctgcggctga ggggccaaat gcagctgttc aagcgccgcg
      661 aggccccgag cgatcaggcc gccgtgcgcg aggccgccaa cctgcgcgcc tattatcaga
      721 gcaagggctt cctgctcgcc agcgtgcgcg gccgctacga ggagttcggc aacaaggccc
      781 ccggcgccct ctacttccag atcaaagagg gcccgaccgt caagatccgc ggcctcgagc
      841 aacggatcac ccgcggcgtg ccacccaagg tcgccgacga catcagccgc gacttcgacc
      901 gcgagcgcaa gatcaagcgc ggcggcagct tcaccgaggc cgacatgatc gacgacctcg
      961 gcgccctgct gcagcgctac caggaccgcg gctacctgtg cgcgcgcgcg aggtttagcg
     1021 tcgccttctg gcccgagggc ctcgaccgcc ccggcgagca cgccgaggtc gacgtcgcca
     1081 cgatcctcaa gcgcgagccg agcgcggcgt gggcgacccg cgacttcgac ccggccggcc
     1141 tgcgccagct gctggcccgc aagcgcgcca acctgtacgt gcgcatcacg atcgagccgg
     1201 gcccgcgcgt gttcacctcg cggcgcaccg agctcgtgcg ctacctcgac gtcccgatcc
     1261 ccggcgaccg caacgtcgcc gacctcccgg tccacaccga tggagcctgg ggcgcgcgcc
     1321 ggatcttctt cgagaccccc ctgcgccgct ccggcgacag ccgccccggc ggcgtcgccg
     1381 tcagcccgag cctcgaccgc gaggtcgagg aggtcgtcgt cgaccgctac cgcgacagcg
     1441 gcttcccggt cgccgacgcc gagatccggt ggctgtacac caacccggcc accaacgccg
     1501 tcatcgccgt gccgacctcg cgccgcctcg ccgacgagga catcggcatg tgcgaggcct
     1561 ataccagcgg caccgccgtc gaggtcgaga ccgagctgtc cgtctacgag ggcaaaaagg
     1621 gcctgttcgg cgacacgctc ctgcgcggca acttcaagac ccgcggctgg accctgcgcc
     1681 ggcagttcgc gtttcgcacc ggcgacgcct acagccgcga ggcgaccgac agcacccgca
     1741 gcaacatcga cggcctcggc gtcgccgaga gcgtcgacat caccccctac ccggtcggct
     1801 gcgagctcga cagcgagggc gactgcgtcg tccaccacgt cgtcgagatg cgcgaggcca
     1861 aggactacgc cgccaccctg tccggcggct tcggcctggc gaccctcgac ccgttctacg
     1921 tgttcttgca gccggccttc ccgaacatct tcggcaccgc ctgggacttc gaggtgctcg
     1981 gtcactacgg cttcaacacc agcaacgtgc ccgcgggcgt gcccttcctc ggcgactgcg
     2041 ccggccagcg ctgctacgag cgcagcgcgc gggccagcct gacgcgccag cggatcttcg
     2101 ccaccccgct gaccttcagc gtcggcggca cgtatcaagt gcgcctgacc ccggcgcgcg
     2161 gccgcatcga gtccgccgtc ggcacgatca ccttcaccta tccgatcacc accaagctcc
     2221 agacctactt cggctacctg atccagcgca gcaacatctc gcaggacgtc gtcaagccgg
     2281 ccttcgaccc ctccgtcccg accgacggcg acgccaacat cgtcaaccgc cgcgacgcga
     2341 tcgtctccga ccgcaccggc gccctgcagc tcggcatgac ctacaccaac gccgacagcc
     2401 cgttcaaccc cgagaagggc ttcatcgccg gcgccgacct caagctcgcc tcgccctacc
     2461 tcggcggcat cgactggttc atccgcgccg acctctcctg gca
//
LOCUS       DASWNJ010001671         2735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_489144, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001671 DASWNJ010000000
VERSION     DASWNJ010001671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489144"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VGB85_27725"
     CDS             complement(<1..810)
                     /locus_tag="VGB85_27725"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0137919.1"
                     /translation="MSGSPDDARDTVQPGPEGLDELDEHGIPELLAGRYRILAHIGEG
                     GMGIVCRGFDLDLEETIAVKFLRADLADDDTLRTRFRREVKLARRVTHPNVARVFEFG
                     RDGPLCFLTMEFVPGESLQVLLARAGMLPPPEVRALAVSLCEALAAAHDADVVHGDIK
                     PGNILIAPGRGAVLTDFGIAQALTDPRRLDEGPSGTPFYMAPEQARGEPIGPQTDVYA
                     VGVVLFEALTGMSPWPDHDAMGQLAYKRQGGEPALAQIAPGLPSVWTQLLAA"
     gene            complement(984..1826)
                     /locus_tag="VGB85_27730"
     CDS             complement(984..1826)
                     /locus_tag="VGB85_27730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY0137920.1"
                     /translation="MILHTLARRSACIAVLLSANLAAAAPPGLAAAPENSSPTLKPAP
                     APAPTGAALDEVQLTSGELVHGRVTNIAEGAYVTMIGAKGTQTFPWTEVARVRFERGA
                     PADAVELDLAAKGFEVDLPVTVDSPYIELQTRDGRPMPLLRLRNSGASVSERYSTGFD
                     EVCRAPCGRAVAAGDRRFFVDSNPYTASKVFEIPRGQKYVRLMVRPGNFRMRRAGVGL
                     MIAGAIGFATSIALIATPGQRTHGAGLMSLASLVPVGGLLFGLSRTKVRVEMMAAPAR
                     AAAR"
     gene            complement(1823..>2735)
                     /locus_tag="VGB85_27735"
     CDS             complement(1823..>2735)
                     /locus_tag="VGB85_27735"
                     /inference="COORDINATES: protein motif:HMM:NF015963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HEY0137921.1"
                     /translation="TLDVEVTYHSDLKLGLGDGPIDYVRDCDYGQLNWFPGQRRVVKT
                     CGVKTDLPRQTGATNRLLAPDFSEWIAGPAERALQHGSCRTAFNCRVLERVRYRQFVN
                     TPLLYRKGEANAPDRSFHNVVGAGNRMMSSELTEVQEGVMQHDLEFAIPAQKAAAVFA
                     AARKHFDAEDLCFPLVGIFIRFSRVDDEAWLAHTSTSDEFHKGDVVAFMEMPIYIPAG
                     FSPERTEAYLRPYKAWVKRLIVEFGARPHWGKNDAWVFDLQDPARAYGARWDTFRSTI
                     AALDPGGLFMNPWLAARGFGRGKDATR"
BASE COUNT          406 a          891 c         1048 g          390 t
ORIGIN      
        1 agcagcgagc agctgcgtcc acaccgacgg tagccccggg gcgatctgcg ccagcgcggg
       61 ctcgccaccc tggcgcttgt acgcgagctg acccatcgcg tcgtgatcgg gccacgggct
      121 catgccggtc agcgcctcga agagcacgac gccgacggcg tagacgtcgg tctgcgggcc
      181 gatcggctcg ccgcgcgcct gctcgggggc catgtagaac ggggtgccgc tcgggccctc
      241 gtcgaggcgg cgcgggtcgg tcagcgcctg ggcgatgccg aagtcggtca gcacggcgcc
      301 gcgccccggg gcgatgagga tgttgccggg cttgatgtcg ccgtgcacga cgtcggcgtc
      361 gtgggcggcc gcgagggcct cgcacaggct gaccgcgagg gcgcggacct cgggcggcgg
      421 gagcatgcca gcgcgcgcga gcaggacctg cagagattcg ccgggcacga actccatcgt
      481 caggaagcac agggggccgt cgcggccgaa ctcgaagacg cgggcgacgt tggggtgggt
      541 gacgcggcgg gcgagcttga cctcgcgacg aaagcgagtg cgcagggtgt cgtcgtcggc
      601 caggtcggcg cgcaggaact tgacggcgat cgtctcctcg aggtcgaggt cgaagccgcg
      661 gcagacgatg cccatcccac cctcgccgat gtgcgcgagg atccggtagc ggccggccag
      721 caattcgggg atgccatgct catcgagctc gtcgaggcct tcgggcccgg gctggacggt
      781 gtcacgcgcg tcgtccggag agccgctcat gtcagcgacg ataacgcatc gtccggggga
      841 cctgccggcc cgccgcgagg gccggcggcg cggcgccgcg cgaggcagcg aggacgggcg
      901 caggatcgac cgcgagcggg cgtgcgtcgg aggggtgcgt cggtgccgag gatgcgtgtg
      961 acgggcacag cggcccgccg agatcagcgc gccgcggccc tcgcgggggc agccatcatc
     1021 tcgacgcgga ccttggtgcg cgacaggccg aacaggaggc caccgaccgg gacgaggctc
     1081 gccagcgaca tcagcccggc tccgtgagtc ctctggcccg gcgttgcgat cagggcgatg
     1141 ctcgtcgcaa agccgatcgc gccggcgatc atcaggccga cgccggcccg acgcatgcga
     1201 aagttgccgg gacgaaccat caggcgtacg tacttctggc cgcggggaat ttcgaacacc
     1261 ttcgaggcgg tatatggatt gctgtcgacg aagaaccggc ggtcgccggc agcgacggcg
     1321 cggccgcacg gggcgcggca cacctcgtcg aagccggtgc tgtagcgctc gctgacgctc
     1381 gcgccgctgt tccgcaggcg cagcaggggc atcggccggc cgtcgcgcgt ctgcaattcg
     1441 atgtacggtg aatcgacggt gaccggcagg tcgacctcga agcctttggc ggcgaggtcg
     1501 agctccaccg cgtccgcggg cgcaccacgc tcgaaccgga cgcgcgcgac ctcggtccac
     1561 gggaaggtct gcgtgccctt ggcgccgatc atcgtcacgt aggcgccctc ggcgatgttg
     1621 gtgacgcgac cgtggacgag ctcgccgctg gtgagctgca cctcgtcgag cgctgccccg
     1681 gtcggcgcgg gcgcgggcgc gggtttcagg gtcggggacg aattctcggg cgcagcggcc
     1741 aggccaggcg gtgcggcggc ggcgaggttc gcggacagca ggacggcgat gcatgccgag
     1801 cggcgcgcca gggtgtggag gatcatcgag ttgcgtcctt tccgcggccg aagccgcggg
     1861 ccgcgagcca ggggttcatg aacaggccgc cgggatcgag cgcggcgatc gtgctgcgaa
     1921 atgtgtccca gcgggcgccg taggcccggg cggggtcttg caggtcgaac acccaggcgt
     1981 cgttcttccc ccagtggggt cgcgcgccga actcgacgat cagccgcttc acccatgcct
     2041 tgtagggccg caggtacgcc tcggtgcgtt cgggggaaaa gccggcgggt atgtagatcg
     2101 gcatctccat gaacgcgacg acgtcgccct tgtgaaactc gtccgacgta ctggtgtgcg
     2161 cgagccaggc ctcgtcgtcg acccgcgaga agcggatgaa gatgccgacc agcgggaagc
     2221 acaggtcctc ggcgtcgaag tgcttgcgcg ccgcggcgaa cacggcggcc gccttctggg
     2281 cggggatcgc gaactcgaga tcgtgttgca tcacgccctc ctgcacctcg gtcagctcgc
     2341 tcgacatcat gcgattgccg gcgccgacga cgttgtgaaa gctgcggtcc ggggcgttgg
     2401 cctccccctt gcggtagagg agcggcgtat tgacgaactg ccggtagcgc acgcgctcga
     2461 gcacacgaca gttgaacgcg gtgcggcagc tgccgtgctg cagcgcgcgc tcggccgggc
     2521 cggcgatcca ctccgagaag tcgggcgcga gcagtcggtt ggtcgcgccg gtctggcgcg
     2581 gaagatcggt cttgaccccg caggtcttga cgacgcggcg ctgcccggga aaccagttga
     2641 gctggccgta gtcgcagtcg cgcacgtaat cgatcggccc gtcgccgagg ccgagcttga
     2701 ggtcgctgtg gtaggtgacc tcgacgtcga gggtg
//
LOCUS       DASWNJ010001672         3399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_489153, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001672 DASWNJ010000000
VERSION     DASWNJ010001672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489153"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..1631
                     /locus_tag="VGB85_27740"
     CDS             147..1631
                     /locus_tag="VGB85_27740"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEY0137922.1"
                     /translation="MATPRPQARGLGTGGISIGFAKLFFLLAGYTVSIYLTRLVSPAT
                     YGDYNVLSQIITIPNMVLIQTLLFAVSRPMSAEHASGLPSYDGLRRRGFRVAALFGGL
                     VAAILFFGAGPIAGYFLQDAALADPLRMVAPIPLIYAMYAVNVGTLNAIRRFQLQATL
                     DIFMAATKAGLIIGLAALGYGLTHILGGFTLASLLVLALSIAFVLWSRPAGAGGGTGP
                     LPPMARFAAVLAVFTAGVNLLQGFDLFVLKRLAVTQDMRDAFGFYSSAALIARVPYSM
                     MNAVSLVMFPLIATLHASRDEAEISRYVGATARISVLLLAFMSSVGASAAPEVQRLLF
                     PAAYASAADELRLLVWGLSGYSLTITSAWIFNSAHRSRAAIGLIAVPLVTVAALTLTL
                     APTRFTAGAALGVAIAGALGAVVSLVALTRSFNARVPLLFFLKLAAAAGLVELAARVW
                     TPVGSLAILVKLAALALVFMLAVAATRAVRLVEIKNLRRRPADA"
     gene            1635..2741
                     /locus_tag="VGB85_27745"
     CDS             1635..2741
                     /locus_tag="VGB85_27745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0137923.1"
                     /translation="MPRPWFIVSKPLRAPFRDGSTVLIRDLVTRMPGDRPLVYLGDPR
                     RPLRTAADTILDHPAMSYSPGLLAKIRVLLAMLHPRRRHLPVQLCFTPNPITSRAVAL
                     LRWLQPRRVLVQSLMSAHGCEAWVPLLRPLDAIVVLSDHTRERLIGAGVDPSRVHRIY
                     PAVASVTADSPDEVAGRQRLLYAGDLDLEVAARLISVAHGLATPELAGWSLTIACRPK
                     SDSDAAARDRLRDALPDELASGRVRLLGEVDDMDALLRGASLQLYAADHVRRKVDLPL
                     VLLEGLARGVPVVAVDADPVRELFTLGARVGLVPGAAAPADPEGFARVVAGLCRPEAL
                     RAASSAAAALAAREFSLDRMVRCYVALHDALEAP"
     gene            2741..>3399
                     /locus_tag="VGB85_27750"
     CDS             2741..>3399
                     /locus_tag="VGB85_27750"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0137924.1"
                     /translation="MPDPVQAPDHPDHPDHPDASADNRAYYDAFSERYDRDRDHGYHK
                     LIDDQAAAIVRRYCADKDVLEVGCGTGLVLKRVAPHTRSARGVDLSPGMLARARERGL
                     EVQEGSATELPFADASFDLSYSFKVLAHIPDWDACMREMVRVTRPGGLLIFDIYNRNS
                     LRYLIKRLWGPRSTSDAFHEGAISTRFWTPAQAAEHLPAGTRILDRFGIRVTTPHPIF
                     AR"
BASE COUNT          408 a         1333 c         1066 g          592 t
ORIGIN      
        1 tcgcccccgc gtctccaccc gcagccacag cacacccgcg tcgtgccaca ccagcgcagg
       61 tgtccctccc aagccacagc ccccatccac ctgtccgccc ccccacgatc cccctgcctg
      121 ccggatcgtg ctagcttgcg cccgcgatgg caacgccacg accccaggcg cgcgggctcg
      181 gcaccggcgg catctcgatc ggcttcgcca agctgttctt tttgctcgcc ggttacaccg
      241 tctcgatcta cttgacgcgc ctcgtctcgc cggcgaccta cggcgactac aacgtcctca
      301 gccagatcat caccattccc aacatggtgc tgatccagac cctgttgttc gcggtgagtc
      361 ggccgatgtc ggccgagcac gcgagcgggt tgccctcgta cgatggcttg cggcggcggg
      421 gctttcgcgt cgcggcgctg ttcggcgggc tcgtcgcggc gatcctcttc ttcggtgccg
      481 ggccgatcgc cggctacttc ctgcaagatg ctgcgctcgc cgaccccttg cgcatggtcg
      541 caccgatccc gctgatctat gccatgtacg cggtcaacgt cggcacgctc aacgcgatcc
      601 ggcggtttca gctgcaggcg accctcgaca tcttcatggc ggcgaccaag gccggtctga
      661 tcatcgggct cgcggcgctg ggttatgggc tcacccacat cctcggtggc ttcacgctcg
      721 cctcgctgct cgtcctcgcg ctctcgatcg cctttgtcct ctggagccgt cctgcgggcg
      781 ccggtggggg caccgggccg ttgccgccga tggcccgctt cgcggcggtg ctcgccgtgt
      841 tcaccgccgg cgtcaacctc ttgcagggct tcgacctgtt cgtgctcaag cgcctcgccg
      901 tcacccagga catgcgcgac gccttcgggt tctactcgag cgccgcgctg atcgcccgcg
      961 tcccgtacag catgatgaac gcggtcagcc tcgtcatgtt cccgctgatc gccacgctgc
     1021 acgccagccg cgacgaggcg gagatcagtc gctacgtcgg ggcgaccgcg cggatcagcg
     1081 tattgttgct cgccttcatg agcagcgtcg gcgccagcgc ggcgccggag gtccagcgcc
     1141 tgctgttccc ggccgcctac gcctccgcgg cggacgagct gcgcctgctc gtgtggggcc
     1201 tgtccggcta ctcgctcacg atcacgtccg cgtggatctt caactcggcg caccgctcgc
     1261 gcgccgcgat cggcctgatc gccgtgccgc tcgtcaccgt cgccgcgctg accctcacgc
     1321 tcgcgccgac gcgcttcacc gccggcgccg cgctcggcgt ggcgatcgcg ggggcgctcg
     1381 gcgccgtcgt cagcctcgtc gccctgacgc gcagcttcaa cgcccgcgtc ccgctgctgt
     1441 tctttttaaa gctcgcggcg gcggccggcc tcgtcgagct cgccgcgcgc gtgtggacgc
     1501 cggtgggcag cctggcgatc ctcgtcaagc tcgccgcgct cgcgctcgtc ttcatgctcg
     1561 ccgtcgccgc cacgcgggcc gttcgactcg tcgagatcaa gaacctgcgg cgaaggccgg
     1621 ccgacgcctg acgcatgccg cggccgtggt tcatcgtctc gaagcctctg cgcgcgccgt
     1681 ttcgcgacgg ctcgaccgtc ctgatccgcg acctcgtcac gcgcatgccc ggtgatcgcc
     1741 cgctggtgta cctcggcgat ccccgccgac ccttacgcac cgccgccgac acgatcctcg
     1801 atcatcccgc gatgtcctac agcccgggcc tgctcgccaa gatccgcgtc ttgctggcga
     1861 tgttgcatcc gcgtcggcgt cacttgcccg tccagctgtg cttcacgccc aacccgatca
     1921 cctcgcgcgc ggtcgccctg ctgcggtggt tacagccgcg acgggtgctc gtgcagagcc
     1981 tgatgtccgc gcacggctgt gaggcctggg tcccgctgct gcgcccgctc gacgcgatcg
     2041 tcgtcctctc cgatcacacc cgcgagcgcc tgatcggcgc cggcgtcgat ccgagccgcg
     2101 tgcaccgcat ctaccccgcg gtcgccagcg tcaccgccga ctcgcccgac gaggtcgcgg
     2161 gccggcagcg cctgctgtac gccggcgacc tcgacctcga ggtcgccgcg cggctgatct
     2221 ccgtcgccca cggcctggcc acaccggagt tggccggctg gtcgctgacg atcgcctgcc
     2281 ggccgaagag tgacagcgac gccgccgcgc gtgaccgtct gcgcgacgcc ttgcccgacg
     2341 agctcgccag cggtcgtgtg cgcctgctcg gcgaggtcga cgacatggac gcgctgctgc
     2401 gtggcgcctc gttgcagctg tatgccgccg atcacgtgcg ccgcaaggtc gacctcccgc
     2461 tggtattgct cgagggtctc gcccgcggcg tgccggtggt cgccgtcgac gccgacccgg
     2521 tgcgtgagct gttcacgctc ggcgcgcgcg tcggtctcgt gccgggagcg gccgcgccgg
     2581 cggacccgga gggcttcgcc cgcgtcgtcg ccggcctgtg tcgtccggag gctctgcgcg
     2641 ccgcctcgtc cgcggcggcg gccctcgcgg cgcgcgagtt ttcgctggat cgcatggtcc
     2701 gatgttatgt tgcgctccac gacgccctgg aggccccctg atgcccgatc cggtccaagc
     2761 tcccgatcat cccgatcatc ccgatcatcc cgacgccagc gccgacaacc gcgcctatta
     2821 tgacgccttc tccgagcgtt acgaccgcga ccgcgaccac ggctatcaca agttgatcga
     2881 cgaccaggcc gccgcgatcg ttcggcgtta ctgcgccgac aaggacgtgc tcgaggtcgg
     2941 ctgtggcacc gggctcgtgc tcaagcgcgt cgcgccgcac acccgcagcg cccgcggcgt
     3001 cgacctctcg cccggcatgc tcgcgcgggc gcgcgagcgc ggcctcgagg tgcaggaggg
     3061 cagcgcgacc gagctgccct tcgcggacgc cagcttcgac ctcagctact ccttcaaagt
     3121 gctcgcgcac atccccgact gggacgcctg catgcgcgag atggtccgcg tcacccgccc
     3181 cggcggcctg ctgatcttcg acatctacaa ccgcaactcc ctgcgttatt taattaaacg
     3241 cctgtggggc ccgcgcagca cctcggacgc cttccacgag ggtgcgatct cgacgcgctt
     3301 ttggaccccc gcgcaggccg ccgagcacct gcccgccggc accaggatcc tcgaccgctt
     3361 cgggatccgc gtcaccaccc cgcacccgat cttcgcgcg
//
LOCUS       DASWNJ010001673         4662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_699761, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001673 DASWNJ010000000
VERSION     DASWNJ010001673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699761"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VGB85_27755"
     CDS             complement(<1..379)
                     /locus_tag="VGB85_27755"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY0137925.1"
                     /translation="MSQPTADPWGERLPRKLGLWSTVAVLIGSTVGSGIFRTPATIAY
                     HIDEVWAFALAWVVGGVVALAGALTFAELASMYPRSGGIYVYVREAFGALPAFLFGWA
                     ELLILRPAAYGAIAVTSSEYAWRV"
     gene            436..1470
                     /locus_tag="VGB85_27760"
     CDS             436..1470
                     /locus_tag="VGB85_27760"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY0137926.1"
                     /translation="MEHEAWIVPLRLRHPFGLSRGTASELPTVLLRLGGRGLGEATPV
                     RYLGHEAEVIRRTLSAVCADLEEPDLEDRRRGAARTRSLAPDLAPVRAAVDIALWDLA
                     ARARGRSLRDEIAAASPVAGAGASETAFTIALDSLDAMAERAAEAGHMPRLKIKLGRD
                     PEFDLTAVDRVRAAAPRARLVVDANGGWSLAQARALLPGLCDRGVELVEQPLARGQLP
                     ALAELRRTSPIPIYADEDVQGPESLAALRGCVDGINVKLMKCGGISPALEMIAMARRE
                     GWRVLLGCMIESRVGLAAAASLAAHVDELDLDAHLLTADDPVPPGNPRVLTATPPAVP
                     GAGLGIPLRW"
     gene            1614..>4662
                     /locus_tag="VGB85_27765"
     CDS             1614..>4662
                     /locus_tag="VGB85_27765"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0137927.1"
                     /translation="MNAPTAPNTLEAAHDTWSETPPAELLEDGAPRRLGRYELLRRIG
                     AGGMGVVYAALDRERGRRVALKTLRKLEPGSLYPLKHEFRAAAGVIHHNLVALYELVS
                     DADQWFITMEYVEGVSFGAYVRSDMSERPDDTVHVAGEEHPVGADQHVTEADAPPLRA
                     PVLDTDRLRDALAQLVSGVNALHAAGKLHRDLKSSNVLVTRSGRLVILDFGLISDRGP
                     APIDVTFDDGILGTPAYMSPEQAAGRPVTAASDWYAVGVMLYEALTGRLPFIGTTLKI
                     LSAKQQHDPLPVHELAPEAPEDLAELCMGLLRRDPSARLTGADLLHRIADVPLLQRRL
                     MSSPSDHAPTLVGREELLSQLHDAHEATKGTPVMVYVHGGSGIGKTALIDHFLGSLRR
                     GDTVVLHGRCYERETVPYKAFDSVVDSITHYLRRLPQLEAAALMPRDLHELTRLFPVL
                     ARVQLVADAPQRAFDGPDQQEARRRAFRGLKELLARIADRKPLVLRVADLQWGDEDSA
                     RLLAELLAPPDAPAMLVIGSYRTEEAAESPMLRELQRLHERPGARGDVRHLFVPPLSQ
                     PQARQLALLRIGRDDEAAQAEATRIAAESEGNPLFVEELVRHAVVSRRLGDNREISME
                     QLVLARLGGLPEPARRLLEVVAVAGRPVPQNMAFATAGLGPEDHNALGVLRAQHLVRS
                     HGARDADTVECYHDRVREAVLAQVDPGTLLAHHRNLGEAFEREGGDPEVTAHHFHAAG
                     LNTRAIRHACLAAERAAAALAFNRSAELYGLAHDWTDPAESAARLNLLNQRADVLVLA
                     GRCGEAAPLYLEAAVGAPPALALELRRQATEQYLVSGRIDEGVKLLRPVLAEVGLQYP
                     PTPSRANMSIITRSTQLSLRGIGFRATHEIDIPAEELLRTDVCLSAGKGLAFVDPVRG
                     HSFVLRGLLLALQIGEPRRVARGLALVGLMMISRGSANSVSRGTTFIKEARKLGDAIA
                     DPYLIALADIISGVAQLALGHWQHSLDLLDSGVRLLHERCAGVA"
BASE COUNT          623 a         1880 c         1550 g          609 t
ORIGIN      
        1 tcactcgcca cgcgtactcc gacgaggtca ccgcgatcgc cccgtaggcc gcgggccgca
       61 ggatcagcag ctcggcccag ccgaacagga acgccggcag cgccccgaag gcctcgcgca
      121 cgtacacgta gatcccgccg ctgcgcgggt acatgctcgc cagctcggcg aaggtcagcg
      181 cgccggccag cgccacgacg ccgccgacca cccacgcgag cgcgaaggcc cacacctcgt
      241 cgatgtgata ggcgatcgtc gccggcgtcc ggaagatgcc gctgccgacg gtcgagccga
      301 tcagcaccgc gaccgtgctc cacagcccga gcttgcgggg caggcgctcg ccccaggggt
      361 cggccgtggg ctggctcatc cgccagcgat accgcatttc tcgccgcgcc ccaagcgaag
      421 cgctagcgtc agcggatgga gcacgaggcc tggatcgtcc ccttgcgcct gcggcacccc
      481 ttcggcctgt cccgcgggac agccagcgag ctgccgaccg tgctcctgcg cctcggcggt
      541 cgcgggctcg gcgaggccac cccggtccgc tacctcggcc atgaggccga ggtgatccgc
      601 cggacgctct ccgcggtctg cgccgacctc gaggaacctg acctcgagga caggcgccgc
      661 ggcgccgccc ggacgcggtc tctggccccc gatctggccc ccgtgcgcgc ggcggtcgac
      721 atcgccctgt gggacctcgc cgcccgcgcc cgcggaagat cactgcgcga cgagatcgcc
      781 gccgcgtccc cggtcgctgg cgccggtgcg agcgagaccg ccttcacgat cgcgctcgac
      841 agcctcgacg cgatggccga gcgggccgcc gaggccggcc acatgccgcg cctcaagatc
      901 aagctcgggc gggatccgga attcgacctc accgccgtcg accgggtgcg ggccgcggct
      961 ccgcgcgcgc gcctcgtcgt cgacgccaac ggcggctggt cgctcgcgca ggcgcgggcg
     1021 ctgctgccgg gcttgtgcga ccgcggcgtc gagctcgtcg agcagccgct ggcgcgcggc
     1081 cagctgcccg cgctcgccga gctgcgacgg accagcccga tcccgatcta cgccgacgag
     1141 gacgtgcagg gcccggagtc gctggccgcg ctgcgcggct gcgtcgacgg catcaacgtc
     1201 aagctgatga agtgcggcgg gatctcaccg gcgctcgaga tgatcgccat ggccagacgc
     1261 gagggctggc gcgtgctgct cggctgcatg atcgagagcc gcgtcggcct cgcggccgcg
     1321 gccagcctgg ccgcccacgt cgacgagctc gacctcgacg cacacctgct caccgccgac
     1381 gacccggtcc ctcccggcaa tccccgcgtg ctcaccgcga cgccccccgc ggtccccggc
     1441 gccggcctcg gcattccact gcgctggtga ccccggctgg ggcccctacc ccccgccgta
     1501 acagcgtcct gcgccggcgc cggcgacatc accggcgcgg cctcttcgct gccgcagccg
     1561 gccgcggcgt ggcatactcg cacgctgccg tgagtgctga agatcccgag cgggtgaacg
     1621 cgccgacggc ccccaacacg ctggaggccg cgcacgacac gtggtccgag acgccgcctg
     1681 ccgagctgct cgaggacggc gcgccgcgtc ggctcggccg ctacgagctc ctgcgcagga
     1741 tcggcgccgg tggcatgggc gtcgtgtatg ccgccctcga ccgcgagcgc ggccgccggg
     1801 tcgcgctcaa gaccctgcgc aagctcgagc ccggcagcct ctaccccctc aagcacgagt
     1861 ttcgggcggc cgccggcgtc atccatcaca acctcgtcgc gctctacgag ctggtctccg
     1921 acgccgacca gtggttcatc accatggagt acgtcgaggg cgtcagcttc ggcgcctatg
     1981 tccgatcgga catgtccgaa cggcccgatg acaccgtgca cgtcgccggt gaggagcacc
     2041 cggtcggcgc cgaccagcac gtcaccgagg ctgacgcgcc gccgctgcgc gccccggtgc
     2101 tcgacaccga ccgcctgcgc gacgcgctcg cgcagctggt cagcggcgtc aatgccttgc
     2161 acgcggcggg caagctgcac cgcgacctca agtcgtcgaa cgtgctggtg acgcgcagcg
     2221 gtcggctggt gatcctcgac tttggcctga tcagcgaccg cggccccgcg ccgatcgacg
     2281 tcaccttcga cgacggcatc ctcggcacgc ccgcgtacat gtcgcccgag caggccgccg
     2341 gccgcccggt gaccgccgcg agcgactggt acgccgtcgg cgtcatgctc tacgaggcat
     2401 tgaccggccg cctgcccttc atcggcacca ccctcaagat cctctcggca aagcagcaac
     2461 acgacccgct gccggtccac gagctcgcgc cggaggcccc cgaggacctc gccgagctgt
     2521 gcatgggcct gctgcgccgt gatcccagcg cgcggctgac cggcgccgat ctcctgcacc
     2581 gcatcgccga cgtcccgctg ttgcagcggc gcctgatgtc gagcccgagc gaccacgcgc
     2641 cgaccctcgt cggtcgcgag gagctgctgt cgcagctgca cgacgcgcac gaggcgacca
     2701 agggcacgcc ggtgatggtg tatgtgcacg gcggctcggg catcggcaag accgcgctga
     2761 tcgaccactt cctcggcagc ctgcgccgcg gcgacaccgt cgtgctgcac ggccgctgct
     2821 acgagcgcga gacggtcccc tacaaggcct tcgacagcgt cgtcgactcg atcacccact
     2881 accttcgtcg gctccctcag ctggaagccg ccgccctgat gccgcgggac ctgcacgagc
     2941 tgacgcgcct gttcccggtg ctcgcgcgcg tgcagctggt cgccgacgcc ccgcagcgcg
     3001 ccttcgacgg ccccgaccag caagaggccc ggcgccgggc gtttcgcggg ctcaaggaat
     3061 tgctcgcgcg gatcgccgac cgcaagccgc tcgtgctgcg cgtcgccgac ctccagtggg
     3121 gcgacgagga cagcgcccgc ctgctcgccg agctgctcgc cccgcccgac gcccccgcga
     3181 tgctcgtcat cggctcgtat cgcaccgagg aggccgccga gtcgccgatg ctgcgcgagc
     3241 tgcagcgcct gcacgagcgc cccggcgccc gcggcgacgt ccgccacctc ttcgtcccgc
     3301 cgctgagcca gccccaggcc cgccagctcg cgctgctgag gataggccgc gacgacgagg
     3361 ccgcccaggc cgaggcgacc cgcatcgccg ccgagtccga gggcaacccg ctgttcgtcg
     3421 aggagctggt gcgccacgcc gtcgtctcgc gtcgcctcgg cgacaaccgc gagatctcga
     3481 tggagcagct ggtgctcgcg cgcctcggcg gcctgccgga gccggcccgc cgcctgctcg
     3541 aggtcgtcgc cgtcgccggc cgacctgtcc cgcagaacat ggcctttgcg acagccggcc
     3601 tcggccccga ggaccacaac gcgctcgggg tcctgcgcgc ccagcacctc gtgagatcgc
     3661 acggagcccg cgacgccgac accgtcgagt gttaccacga ccgcgtccgc gaggccgtgc
     3721 tcgcgcaggt cgaccccggc acgctgctcg ctcaccaccg caacctcggc gaggccttcg
     3781 agcgcgaggg cggcgacccc gaggtcaccg cgcatcactt ccacgccgcc ggcctcaaca
     3841 cccgcgcgat ccgccacgcc tgcctcgcgg ccgagagggc cgcggccgcg ctggccttca
     3901 accgctccgc cgagctctac ggcctcgccc acgactggac cgaccccgcc gagtccgccg
     3961 cgcgcctcaa cctcctcaat cagcgcgccg acgtgctcgt gctcgccggt cgctgcggcg
     4021 aggcggcgcc gctgtacctc gaggccgccg tcggcgcccc gccggccctc gcgctcgagc
     4081 tgcggcgcca ggcgaccgag cagtacctcg tcagcggccg catcgacgag ggcgtcaagc
     4141 tgctgcgtcc ggtgctcgcc gaggtcggcc tgcagtaccc gccgaccccg agccgcgcca
     4201 acatgtcgat catcacccgc tcgacgcagc tgagcctgcg cgggatcggg ttccgggcga
     4261 cccacgagat cgacatcccg gcggaggaat tgctgcgcac cgacgtgtgc ctctcggccg
     4321 gcaagggcct cgccttcgtc gaccccgtgc gcggccacag cttcgtgctg cgcggcctgc
     4381 tgctcgccct gcagatcggc gagccgcgcc gggtcgcccg cggcctcgcg ctcgtcggcc
     4441 tgatgatgat ctcccgcggc agcgccaact ccgtgtcgcg cgggaccact tttattaaag
     4501 aggcgcgcaa gctcggcgac gcgatcgccg acccctacct gatcgccctc gccgacatca
     4561 tctccggcgt cgcccagctc gcgctcggcc actggcagca ctcgctcgac ctgctcgact
     4621 ccggcgtgcg cctgcttcac gagcgctgcg ccggcgtcgc ct
//
LOCUS       DASWNJ010001674         1684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_277926, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001674 DASWNJ010000000
VERSION     DASWNJ010001674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277926"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VGB85_27770"
     CDS             <1..1182
                     /locus_tag="VGB85_27770"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0137928.1"
                     /translation="LASRLVIDWHPPDSPRLRAATSILAEQLPADIDARDSELRRLGE
                     RLDAALTLWDPAGGVLATTGRPLAPPQLAGPPTQWSHPDHGLVLVTRLDDGRWLGVGL
                     DAGDDLARFTHFSLLLGFAILLSAALCYPIARRITRRVEDLRRGVEGLGAGDLGSRVQ
                     VQGRDEVAALATQFNRAADRIEALVAGQRRMLASASHELRSPLARLRMAVELLADAAP
                     QQHALASEAGRDIEELDALVGDLLLSSRLEARPDIAHTPVDLAALVADEATRAGASNL
                     SSGTAELRGDPAALRRLIRNLLENARAHGEPPIEVTVEATTTGVRLVVEDRGPGVPEA
                     ERERIFAAFYRPAGHREGDGGVGLGLALVRQISALHEGVVRCEARPGGGSRFVVELPR
                     G"
     gene            complement(1203..>1684)
                     /locus_tag="VGB85_27775"
     CDS             complement(1203..>1684)
                     /locus_tag="VGB85_27775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137929.1"
                     /translation="AGEVGAATCLEWLLFQRQAERLPAPMLARPTELSAYVLRGHGRV
                     RVYLSGDDRVGGRLRGEVTERVVADVAAGYVPVAHLHNHPFMFDRTPGDRTYATADTM
                     ADIAGALAPSLTDVQVYRSMRAHFGLRGAWVTNGLDTARYSAADFDRLSAWPPNDAQ"
BASE COUNT          206 a          677 c          594 g          207 t
ORIGIN      
        1 ctggcgagcc gcctggtgat cgactggcac ccgcccgaca gcccgcgcct gcgcgccgcc
       61 acgagcatcc tcgccgagca gctgcccgcc gacatcgacg cccgggacag cgagctgcgc
      121 cgcctcggcg agcgcctcga cgccgcgctg accctgtggg accccgcggg cggcgtgctc
      181 gcgaccaccg gccgcccgct cgcgccgccg cagctcgccg ggccgccgac gcagtggtcg
      241 cacccggacc acggcctcgt gctggtgacg cgcctcgacg acgggcgctg gctcggcgtc
      301 ggtctcgacg ccggtgacga cctcgcgcgc ttcacccact tctcgctgct gctcggcttt
      361 gcgatcctgc tcagcgccgc cctgtgttac ccgatcgcgc gacggatcac ccggcgcgtc
      421 gaggacctgc ggcgcggcgt cgagggcctc ggcgccggtg acctcggcag ccgcgtgcag
      481 gtgcaaggtc gcgacgaggt cgccgcgctc gccacccagt tcaaccgcgc cgccgaccgc
      541 atcgaggcgc tcgtcgccgg gcagcggcgc atgctcgcca gcgcctcgca cgagctgcgc
      601 tcgccgctcg cccgtttgcg catggccgtc gagctgctcg ccgacgccgc cccgcagcag
      661 cacgcgctcg ccagcgaggc cgggcgcgac atcgaggagc tcgacgcgct cgtcggcgac
      721 ctgctgctca gcagccgact cgaggcccgc cccgacatcg cccatacacc ggtcgacctc
      781 gccgccctgg tcgccgacga ggcgacgcgg gccggcgcga gcaacctgtc ctccgggaca
      841 gccgagctgc gcggcgaccc tgccgccctg cgccggctga tccgcaactt gctggagaac
      901 gcgcgcgcgc acggcgagcc gccgatcgag gtcaccgtcg aggccaccac gaccggcgtc
      961 cgcctcgtcg tcgaggaccg tggccccggc gtgccggagg ccgagcgcga gcgcatcttc
     1021 gcggcctttt atcgccccgc gggccaccgc gagggcgacg gcggggtcgg gctcgggctc
     1081 gcgctggtcc gccagatctc cgcgctgcac gagggcgtcg tgcgctgcga ggcgcggccc
     1141 ggcggcggga gccgcttcgt cgtcgagctg ccgcgcgggt gagcctcgcg gcggatccag
     1201 cctcactgcg cgtcgttcgg cggccacgcc gacaggcgat cgaagtccgc cgccgagtag
     1261 cgggcggtgt cgaggccgtt ggtcacccac gccccgcgca ggccgaagtg cgcgcgcatc
     1321 gagcgatata cctgcacatc ggtgaggctc ggcgcgagcg ccccggcgat gtccgccatc
     1381 gtgtccgcgg tcgcgtaggt gcggtcgccg ggcgtgcggt cgaacatgaa gggatgatta
     1441 tgcaagtgcg cgaccgggac gtaccccgcg gcgacgtcgg cgaccacccg ctccgtcacc
     1501 tcgccgcgca gccggccgcc gacgcggtcg tcccccgaca gatatacgcg cactctcccg
     1561 tgcccgcgca gcacgtacgc gctgagctcg gtcggccgcg ccagcatcgg cgccggcaaa
     1621 cgctccgcct gacgctggaa cagcagccac tcgaggcagg tcgccgcgcc gacctcaccg
     1681 gcct
//
LOCUS       DASWNJ010001675        11008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_208102, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001675 DASWNJ010000000
VERSION     DASWNJ010001675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 11008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11008
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208102"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VGB85_27780"
     CDS             <1..337
                     /locus_tag="VGB85_27780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137930.1"
                     /translation="ELLGTASNAAYFKPWMAFYFFENNFSGRLDIEYAVAHQRAATVG
                     NKANMGLEIDAAMRYHDQQEPIFVQLQYGVMFPFGAFNRDLSTFPTAQNGGDAKAAQT
                     IQAQIGIKF"
     gene            384..1778
                     /gene="radA"
                     /locus_tag="VGB85_27785"
     CDS             384..1778
                     /gene="radA"
                     /locus_tag="VGB85_27785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEY0137931.1"
                     /translation="MSKAKKAAPRWVCQACDGVTSGWFGKCPTCDAWNTIESEAPAPA
                     PGERPSGHSTVVASTDAQTQVDSQPRRPCGMNEVDRVLGGGLVPGSVVLLGGPPGIGK
                     STLLLQASVGLARRAGLVLYASGEESVAQVGSRCARLAASHKNLLLMAEQRIEEVLGQ
                     ATERVKTLGALIVDSVQTVYSGTQDGLPGNITQIRAVTSQLVAFAKHHGVPVVVVGHV
                     TKDGQLAGPRLLEHLVDAVLSFEGDEERATRLLRATKNRFGSTQELGVFEMRGDGLRE
                     IANPSEAFLTNRDAPAIGSCVTATLEGSRPLLLEVQALLAASFGNPRRTCVGVDPIRL
                     AMLMAVLDRHAGLFVLDQDVFVNVVGGMRLSEPASDLAVLLAIASSHRRQPLPVGMIA
                     FGEIGLTGELRPVPRSEARVQEAARLGLKKVLMPAGPKQAARTLKDNLENLPKGVELL
                     FARTVAEAIELAFA"
     gene            complement(1783..2475)
                     /locus_tag="VGB85_27790"
     CDS             complement(1783..2475)
                     /locus_tag="VGB85_27790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137932.1"
                     /translation="MLQGEGAGGNNCIIFGDDGMDTKCVDRNAGMASALPLVSYEGDP
                     MQLKDDLVNVFGARFDVPVIEYKGEVVPPAAFATAVFSVTVFRTTEALPELDVFPMGP
                     GNPWTEGTNKEPKPCMAGEPTYRCRACSEPPDPESCGEPWAYGTVFNPNHPMGEMIGT
                     MSTNVQPNTQGLAKLELMSPGQVVRAFQGLVVSAQPDLVAGDKFQVKTRDNSATTAPT
                     LSIMFCPNPNNE"
     gene            2564..2800
                     /locus_tag="VGB85_27795"
     CDS             2564..2800
                     /locus_tag="VGB85_27795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137933.1"
                     /translation="MLVPSPVSVVGSRPVLVDVSLPLTPGPCVVTGSAVLVLVLVVGD
                     VSVVVIIVTPLTLVVCAALSLSGSESLSLKPGLS"
     gene            3062..4525
                     /locus_tag="VGB85_27800"
     CDS             3062..4525
                     /locus_tag="VGB85_27800"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HEY0137934.1"
                     /translation="MSALPVTPEAPIDLAEVYLRSTEAVTEEVDVSLPVIEGAVPAGL
                     RGVLFRNGPGRMELYGQRYEHPFDGDGHLNRFEISDTGIHYRNRYVRTREFLEEEAAR
                     KILYRNFGTNIPGGFHKNLLRMRFKNAANTSVVMHAGRLHALWEGGWPHRIDPVTLDT
                     LARDDLGGALRNDRSFIDRLIAPELPYSAHPRLDPATGELFNFGVGSGREPALMLYHL
                     SPEGICVRRERMPLPTMGFIHDFTLTRNHRVFFLTPTTFDLPRMMLGLATPVDTLRSR
                     PEPTQIFVLPRRGAQARMFTARPCFIFHFTNAFEDGPHIVVDAYVMDAMPASGIAAKL
                     LAGSAEQFPKPRLTRFILDTRSGAVEERQLSEYAGELGTINPAHWTTPHRYSWSIGSP
                     VDRRSPFHTGIVKVDAETRTSNLRDFDPVLTGEPIFVARPGATAEDDGWILSMTYDGV
                     DHRGELLVLDAADLHTVCRARMPHHSPLGFHGTWVAA"
     gene            complement(4607..5821)
                     /gene="recA"
                     /locus_tag="VGB85_27805"
     CDS             complement(4607..5821)
                     /gene="recA"
                     /locus_tag="VGB85_27805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEY0137935.1"
                     /translation="MANVPTTTTPVSQRDSAIDLALATIEKQFGKGSIMRLSKDGKLS
                     TGDISVIPTGALPLDIALGIGGLPRGRIVEIYGPESSGKTTLTLHAIAEAQRRGGVCA
                     FIDAEHALDIGYARKIGVRTDDLLVSQPDFGEQALEICDTLVRSGAIDIIVVDSVAAL
                     TPKAELEGEMGDSHVGLQARLMSQALRKLTGSVARSRTMVIFINQLRMKIGVMFGSPE
                     TTTGGNALKFYASIRLDIRRIGAIKEGEQVVGNRTRVKVVKNKLAPPFREVEFDITYG
                     EGVSREGAIIDLGVEDKVIEKSGAWLSYKGDRIGQGRENAKKFLRENADICMKIEAEI
                     LVKHNIVQNTGTLVAAQNEAAGKGADKGEGKPKPDASPVKVVAAAATATATAAADDDK
                     KGKPAPVKAANK"
     gene            complement(6062..7378)
                     /locus_tag="VGB85_27810"
     CDS             complement(6062..7378)
                     /locus_tag="VGB85_27810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HEY0137936.1"
                     /translation="MVQAQILTIGDELLSGDTVNSNLAFLGERCRRLGVSVIRAVSVR
                     DRIDEIVAALADVARWADLCLVSGGLGPTSDDLTAEAVAHACGVPLVRNEAAVATMVE
                     MFARFNRPMPEINRKQADLPSGADMLLNPIGTAPGFAVHLPDSAARGRKCWIACMPGV
                     PREMRRMMVEQIEPRLARDFELPAVHRRVYRAIGRGESAVAVDIEPIVAEARRRSPGL
                     ANLYLHYRAAAPQVLIIAEGTSGPDGARATMEELAELDAPFTKALQPALYGLGEADLP
                     ARVIAALTRAGKTVGSAESCTGGRVGALLTEVAGASACYFGGVIAYDNTVKSGVLGVS
                     EVDLSQHGAVSEVVARAMAEGARRVLGTDFAVATTGIAGPGGGSKEKPVGTVDIAVAD
                     AEGCTYKRLQLHGERALIQHVSALWALKLLWDRLLERGLASIELMD"
     gene            7454..8227
                     /locus_tag="VGB85_27815"
     CDS             7454..8227
                     /locus_tag="VGB85_27815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEY0137937.1"
                     /translation="MKAIVRLRYAAKTDVGMKRAHNEDYFSLIEDEKVFLVADGMGGH
                     ACGEVASKLSGDVISEFYQHSKDMDATWPYRYDPGLSYPENRMVAAVRLANARIFDAA
                     QKNPNLRGMGTTIVGCMFVGDYVYVAHVGDSRCYRVSQDGIKQLTRDHSLLEDYKDAR
                     PDMSEEEARNFPHKNVITRALGMRDTVVVDISKFEIKDGDQFVLCSDGLCGMLDDKTI
                     GRIVREASTLENAVAELIEQANRAGGTDNITAMLIQCNL"
     gene            8235..8642
                     /locus_tag="VGB85_27820"
     CDS             8235..8642
                     /locus_tag="VGB85_27820"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02266 family protein"
                     /protein_id="HEY0137938.1"
                     /translation="MTDPESSTTEGNRRSEPRLPVHLEVDCSSAGTGDTFLYAFITDI
                     SSMGIFIRTDEPQPIGTMLELGFTPPPDSKSELPPRRLELTGEVMWNTKGSETGAPGM
                     GVRFHHTSPKTRQRLLEIVRAIAYLDGSETPAS"
     gene            complement(8646..9677)
                     /gene="selD"
                     /locus_tag="VGB85_27825"
                     /pseudo
     CDS             complement(8646..9677)
                     /gene="selD"
                     /locus_tag="VGB85_27825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
     gene            9797..>11008
                     /gene="selB"
                     /locus_tag="VGB85_27830"
     CDS             9797..>11008
                     /gene="selB"
                     /locus_tag="VGB85_27830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEY0137939.1"
                     /translation="MARSTRALVLGTAGHIDHGKTSLVYALTGIDTDRLPEEKRRGIT
                     IELGFAAWELGGGLSASIVDVPGHEAFVRTMVAGAGGIDLVLLVVSAEDGVMPQTREH
                     LHVCQLLGLRHGVVALTKIDRLGTANDDRADAIEMAAADVRAALADLAPNLADAPIVP
                     CSAHTGEGLPALTAAIRRVAATVPARPVRDRPIVPIDRVFAVKGHGTVATGTLLSGRL
                     DLTRDARLQLIPTGERGPRELRPRALHVRGVASEQAPAGSRVALNLGNVGTDDLARGD
                     VISSGPRVARGQTCLAVLTHLAHDDLPWRSGTAVQLCAGTASTTARIDPLVLLGDMSE
                     ETSLHAPVEATRPPPDLSPGTSPATAPPGAPPDLSQSTSTRVTVPPGATALVRLRLDA
                     PLPLWHGLPLIV"
BASE COUNT         1673 a         4228 c         3437 g         1670 t
ORIGIN      
        1 cgagctgctc ggcaccgcct ccaacgcggc gtacttcaag ccgtggatgg cgttctactt
       61 cttcgagaac aacttctcgg gccgcctcga catcgagtac gccgtcgccc accagcgcgc
      121 cgcgacggtc ggcaacaagg cgaacatggg cctcgagatc gacgccgcga tgcgctacca
      181 cgaccagcag gagccgatct tcgtgcagct gcaatacggc gtgatgttcc ccttcggcgc
      241 cttcaaccgc gacctctcga ccttcccgac cgcgcagaac ggcggcgacg ccaaggccgc
      301 ccagacgatc caggcccaga tcggcatcaa gttctagcgc cgtcgccttg cacgcgcgga
      361 tccgtgaggc agactcaccc gccatgagca aggccaaaaa agccgcaccg cgctgggtct
      421 gccaggcctg cgacggggtc acctcgggct ggttcggcaa gtgcccgacc tgcgacgcct
      481 ggaacacgat cgagtccgag gcccctgcgc ccgccccggg cgagcggccc tccggccact
      541 ccaccgtcgt cgcctcgacc gacgcccaga cccaggtcga ctcccagccg cgccgaccct
      601 gcggcatgaa cgaggtcgac cgcgtgctcg gcggcggcct cgtgcccggc agcgtcgtgc
      661 tgctcggcgg gcccccgggg atcggcaagt cgaccctgct gctgcaagcg agcgtcggcc
      721 tggcccgccg cgccggcctc gtgctctacg ccagcggcga ggagtcggtc gcccaggtcg
      781 gctcgcgctg cgccaggctg gccgcgagcc acaagaattt attgttgatg gccgagcagc
      841 ggatcgaaga ggtgctcggc caggcgaccg agcgcgtcaa gaccctcggc gccctgatcg
      901 tcgactcggt ccagaccgtc tacagcggca cccaggacgg cctgcccggc aacatcaccc
      961 agatccgcgc cgtcaccagc cagctcgtcg cgttcgccaa gcaccacggc gtgccggtcg
     1021 tcgtcgtcgg tcacgtcacc aaggacggcc agctcgccgg cccgcgcctg ctcgagcacc
     1081 tcgtcgacgc cgtgctctcc ttcgagggcg acgaggagcg cgcgactcgc ctgctgcgcg
     1141 ccaccaagaa tcgcttcggc agcacccagg agctcggcgt gttcgagatg cgcggcgacg
     1201 gcctccgcga gatcgccaac ccctcggagg cctttctcac caaccgcgac gcccccgcga
     1261 tcggctcgtg cgtcacagcc acgctcgagg gctcgcgtcc gctgttgctc gaggtccagg
     1321 cgttgttggc agccagcttc ggcaacccgc ggcgcacctg cgtcggcgtc gacccgatcc
     1381 gcctcgccat gctgatggcc gtgctcgacc gccacgccgg cctgttcgtg ctcgaccagg
     1441 acgtctttgt caatgtcgtc ggcggcatgc gcctctcgga gcccgccagc gacctcgccg
     1501 tgctgctggc gatcgcgtcg agccaccgcc gccagccgct gccggtcggc atgatcgcct
     1561 tcggcgagat cggcctcacc ggcgagctgc gccccgtccc acgcagcgag gcccgcgtgc
     1621 aagaggccgc ccgcctcggt ttgaaaaagg tcctgatgcc cgccggcccc aaacaggccg
     1681 cccgcaccct caaggacaat ttggagaatt tgccgaaggg cgtcgagctg ctgttcgccc
     1741 gtactgtcgc ggaggcgatc gagctcgcct tcgcgtgaag ctctactcgt tgttggggtt
     1801 cggacaaaac atgatcgaca gggtcggcgc ggtagtggcc gagttgtcac gcgtcttgac
     1861 ctggaacttg tcgcctgcca ccagatccgg ctgggctgac accaccaaac cctggaacgc
     1921 tcggacgacc tgccccggac tcatcagctc gagcttcgcg agcccctgcg tgttcggctg
     1981 gacattcgtc gacatcgtcc caatcatctc ccccatcgga tggttcgggt taaagactgt
     2041 gccgtaggcc cagggctcac cacaactctc tggatcgggt ggctcgctac aagcccgaca
     2101 ccggtacgtc ggctcccccg ccatgcaggg ctttggctcc ttgttcgtcc cctcggtcca
     2161 ggggtttcct ggtcccatgg gaaacacgtc gagctcgggg agagcctccg tggtgcggaa
     2221 caccgtcacc gagaataccg ccgtggcgaa cgcggctggc ggcaccacct cgcccttata
     2281 ctcgatgacc ggcacgtcga agcgtgcgcc gaacacgttc accagatcat ccttgagctg
     2341 catcgggtca ccttcgtagc tgaccagcgg cagagcgctc gccatgccgg cgttacggtc
     2401 gacacatttg gtgtccattc cgtcgtcacc aaagatgatg cagttattgc cgccagcacc
     2461 ttcgccctga agcaagaaga aaccatccgc ggccgcgggc accggctccg cgggttgaag
     2521 cttgttcgga tcgcagctcg cgtcccacgg caggaggtca cccgtgctcg tgccctcccc
     2581 cgtctccgtc gtcggatcga ggcccgtgct cgtcgacgtc tcgctaccgc tgacccccgg
     2641 cccctgcgtc gtcaccggct ccgccgtgct cgtgctcgtg ctcgtcgtcg gcgacgtctc
     2701 tgtcgtcgtc atcatcgtca cgccgctgac gctcgtcgtc tgcgccgcgc tgtcgctctc
     2761 cgggtccgag tccttgagct tgaagcccgg gttgtcgtag ccacagccgg gggctgcgcc
     2821 cgtcagggtc acgaggacga ccgttcgcgc cgctgtccgg aggctccgca tcgaccttct
     2881 acgctaccac agaccctccg tccggcgagc acgccggagc gcctcggaga ccccgcgcac
     2941 gctccgcaac gttctcctgg ccgctcgccg gtcgctcttg cccggtcccc ggggccgggc
     3001 tcggacgcgc ccgctgggcc tcgctggtaa tttccgggga ctgcgactac accgcgtttc
     3061 gatgtccgcg ctgcctgtca cgcccgaggc gccgatcgac ctcgccgagg tctacctgcg
     3121 atcgaccgag gccgtcaccg aggaggtcga cgtctcgctg ccggtgattg aaggcgccgt
     3181 acctgccggc ctgcgcgggg tcctgttccg caacggtccc ggcaggatgg agctgtacgg
     3241 tcagcgctac gagcacccct tcgacggcga cggccacctc aaccgcttcg agatctccga
     3301 caccgggatc cactatcgca accgctacgt gcgcacgcgc gagttcctcg aggaggaggc
     3361 cgcgcgcaag atcctctacc ggaactttgg caccaacatc cccggcggct tccacaagaa
     3421 cctgctgcgc atgcgcttca aaaacgccgc caacacctcc gtggtgatgc acgccggtcg
     3481 cctgcacgcc ttgtgggagg gcggctggcc gcaccgcatc gaccccgtca cgctcgacac
     3541 cctcgcgcgc gacgacctcg gcggagcgct gcgcaacgat cgctcgttca tcgaccgcct
     3601 gatcgccccc gagctgccct actcggccca cccgcgcctc gaccccgcca ccggcgagct
     3661 gttcaacttc ggcgtcggct ccggccgcga gccggcgttg atgctctacc acctcagccc
     3721 cgagggcatc tgcgtccgcc gcgagcgcat gccgctgccg acgatgggct ttatccacga
     3781 ctttacgctc accaggaacc accgcgtctt cttcctgacg ccgaccacct tcgacctccc
     3841 gcgcatgatg ctcggcctgg cgacccccgt cgacacgctg cgctcgcgcc ccgagccgac
     3901 ccagatcttc gtgctgccgc gccgcggggc ccaggcgcgc atgttcaccg cgcgcccctg
     3961 cttcatcttc cacttcacca acgccttcga ggacggcccg cacatcgtcg tcgacgccta
     4021 cgtcatggac gcgatgccgg cctccgggat cgccgccaag ctcctggcgg gcagcgccga
     4081 gcagttcccg aagccgcggc tgacccgctt catcctcgac acccgcagcg gcgccgtcga
     4141 ggagcgccag ctcagcgagt atgccggcga gctcggcacg atcaacccgg cccactggac
     4201 caccccgcac cgctacagct ggtcgatcgg cagcccggtc gatcgccgct cgcccttcca
     4261 cacggggatc gtcaaggtcg acgccgagac ccgcacgagc aaccttcgcg acttcgatcc
     4321 cgtgctcacc ggcgagccga tcttcgtcgc ccgtcccggc gccactgccg aggacgacgg
     4381 ctggatcctc agcatgacct acgacggcgt cgaccaccgc ggcgagctgc tcgtgctcga
     4441 cgccgccgac ctgcacaccg tctgccgcgc ccgcatgccg caccacagcc cgctcggctt
     4501 ccacggcacc tgggtcgccg cgtaaatttc gttagagaaa aataaaaagg gccgcgcggc
     4561 ggaccgcgcg accccttcgc aagaacagcc acgtacccgc gaggacctac ttgttggccg
     4621 ccttgaccgg cgccggcttg cccttcttgt cgtcgtcggc cgctgcggtg gcagttgcgg
     4681 tggcagccgc ggcgacgacc ttgaccggcg acgcgtccgg cttcggcttg ccctcgccct
     4741 tgtcggcgcc cttgcccgcc gcctcgttct gcgcggcgac cagcgtgccg gtgttctgca
     4801 cgatgttgtg cttcaccagg atctccgcct cgatcttcat gcagatgtcg gcgttctcgc
     4861 gcaggaactt cttggcgttc tcgcggccct ggccgatgcg gtcgcccttg tacgacagcc
     4921 acgcgcccga cttctcgatc accttgtcct cgacgccgag gtcgatgatc gcgccctcgc
     4981 gcgacacgcc ctcgccgtag gtgatgtcga actcgacctc gcggaacggc ggggccagct
     5041 tgttcttcac gaccttgacg cgggtccggt tgccgacgac ctgctcgccc tccttgatcg
     5101 cgccgatgcg gcggatgtcg aggcggatcg acgcgtaaaa cttgagcgcg ttgccgccgg
     5161 tggtcgtctc cgggctgccg aacatgacgc cgatcttcat gcgcagctga ttgatgaaga
     5221 tgaccatcgt gcgcgaccgg gcgaccgagc ccgtcagctt gcgcagcgcc tggctcatca
     5281 ggcgcgcctg caggccgacg tggctgtcgc ccatctcgcc ctcgagctcg gccttggggg
     5341 tcagcgccgc gaccgagtcg acgacgatga tgtcgatcgc gccggagcgg accagcgtgt
     5401 cgcagatctc gagcgcctgc tcgccgaagt ccggctgcga gaccagcagg tcgtccgtgc
     5461 gcacgccgat cttgcgggcg tagccgatgt cgagcgcgtg ctcggcgtcg atgaaggcgc
     5521 acacgcctcc gcggcgctgg gcctcggcga tcgcgtgcag ggtcagggtc gtcttgcccg
     5581 atgactccgg gccgtagatc tcgacgattc gaccgcgcgg gaggccgccg atgccgagcg
     5641 cgatgtccag cggcagcgcg cccgtcggga tcacgctgat gtcgcccgtc gacagcttgc
     5701 cgtccttgct caggcgcatg atggagccct tgccgaactg cttctcgatc gtcgccaggg
     5761 ccagatcgat cgcggagtct cgctggctca cgggagtggt ggtggtcggt acgttggcca
     5821 ttactttgtg tcctcggcgc gcaacttagc tgcagcgcat gatctatagg agaacaagtg
     5881 ttccgtgtcg agcgggaacg accgcccgac gcttgacacc ccgggcctgc tcggcccccg
     5941 cgcaacccct tcaggggcgg ggcaacgacc gggcagtgat cccagcccgg gccgtgtgcg
     6001 ctgacccggc atcgtccgga tcttcggcga tcgcatcatc ttcgtcacga tcgatcgccc
     6061 gtcaatccat cagctcgatg ctcgccaggc cgcgctccaa aagccggtcc cacagcagct
     6121 tgagggccca cagcgccgag acgtgttgga tcaaggcgcg ctcgccgtgc agctgcagcc
     6181 gcttgtacgt gcagccctcg gcgtcggcga ccgcgatgtc gacggtgccg accggcttct
     6241 ccttcgaacc accgcccggt cccgcgatcc ccgtggtcgc gaccgcgaag tcggtgccga
     6301 gcactcgcct cgccccctcg gccatcgccc gcgcgaccac ctcggacacc gctccgtgct
     6361 gcgagagatc tacctcagaa acgccgagta cccccgattt caccgtgttg tcgtaggcga
     6421 tcacgccgcc gaaatagcag gctgacgcgc ccgcgacctc ggtcagcagg gccccgacgc
     6481 gaccccccgt gcacgactcc gccgacccga ccgtcttgcc cgcccgggtc agcgccgcga
     6541 tcacccgcgc cggtagatct gcctccccga ggccgtagag ggccggctgc agcgctttcg
     6601 tgaacggggc gtcgagctcg gccagctcct ccatcgtcgc ccgcgccccg tcgggccccg
     6661 acgtgccctc ggcgatgatc aacacctgcg gcgccgcggc ccggtaatga agatagaggt
     6721 tggcgaggcc cggagaacgc ctgcgggcct cggccacgat cggctcgatg tccaccgcga
     6781 cggccgattc gccgcggccg atcgcccggt acacccggcg atgcaccgcc ggcagctcga
     6841 agtcccgggc cagccgcggc tcgatctgct cgaccatcat ccgccgcatc tcccgcggaa
     6901 cgcccggcat acaggcaatc cagcactttc ggccgcgcgc ggccgagtcc ggcaagtgca
     6961 cggcaaagcc gggcgccgtg ccgatcgggt tgagcaacat gtcggccccg gacggcagat
     7021 ccgcctgctt gcggttgatc tccggcatcg ggcgattaaa cctggcgaac atctccacca
     7081 tggtcgccac cgccgcctcg ttgcggacca gcggcacccc gcaagcgtgg gccaccgcct
     7141 cggccgtcag gtcgtccgag gtcggcccga gcccgcccga gaccaggcag agatcggccc
     7201 agcgggccac atcggccagc gcagcgacga tctcgtcgat ccggtcgcgc accgaaaccg
     7261 cccggatcac cgacaccccg agccgccggc agcgctcccc gaggaaggcg aggttgctgt
     7321 tcaccgtgtc accggacaac aattcgtcgc cgatggtgag gatctgggcc tggaccatgc
     7381 gcccgggaaa ctaccacgag cgctcccgcg cgcgatgcgc cgccccttcc agggccggcg
     7441 tcgctcggct aggatgaaag ccattgtgcg acttcgctac gccgccaaaa ccgatgtggg
     7501 catgaagcgc gctcacaacg aggattactt ctcgctcatc gaagacgaga aggtcttcct
     7561 cgttgccgac ggcatgggtg gccacgcctg cggcgaggtc gccagcaagc tgtctggtga
     7621 cgtgatcagc gagttctacc agcacagcaa ggacatggac gccacgtggc cgtatcgcta
     7681 cgaccccggg ctgtcctatc cggagaaccg catggtcgcc gcggtgcgcc tcgccaacgc
     7741 gcgcatcttc gacgctgccc agaagaaccc caacctgcgc ggcatgggca cgacgatcgt
     7801 cggctgcatg ttcgtcggcg actacgtgta cgtcgcccac gtcggcgact ctcgctgcta
     7861 ccgcgtgtct caggacggca tcaagcagct gacgcgcgac cactcgctgc tcgaggacta
     7921 caaggacgcg cgccccgaca tgtccgagga ggaggcgcgc aacttccccc acaagaacgt
     7981 catcacgcgc gcgctcggca tgcgtgacac ggtggtcgtc gacatctcga agttcgagat
     8041 caaagacggc gaccagttcg tcctctgctc cgacggtctg tgcggcatgc tcgacgacaa
     8101 gacgatcggc cgcatcgtcc gcgaggcgag caccctcgag aacgccgtcg ccgagctcat
     8161 cgagcaggcc aaccgcgccg gcggcaccga caacatcacc gcgatgctca tacagtgtaa
     8221 cttgtgagcc acccgtgaca gaccccgaga gcagcaccac cgagggcaac cgccgcagcg
     8281 agccgcgcct cccggtgcac ctcgaggtcg actgcagctc ggccggcacc ggcgacacgt
     8341 tcctctacgc gttcatcacc gacatctcgt cgatgggcat cttcatccgc accgacgagc
     8401 cccagcccat cggcacgatg ctcgagctcg gcttcacgcc gccccctgac agcaagagcg
     8461 agctaccccc ccgccgcctc gagctgaccg gcgaggtcat gtggaacacc aagggcagcg
     8521 agaccggcgc cccgggcatg ggcgtccgct tccaccacac ctcgcccaag acccgccagc
     8581 ggctgctcga gatcgttcgc gcgatcgcct acctcgacgg cagcgagacg cccgccagct
     8641 gacagctacg gcccggcgtg gtcgagggtc atcgtgccgg ccggagtgtc ggcatccggc
     8701 gcgctgagct ggccgatcgg gcagccgagc tgcccgcgcg cgcgcagctc gcgacacagc
     8761 ccctcggccc ggtcggcttc gacacacagc agcagcccgc cgctggtctg ggcgtccgcg
     8821 gcgagcagcg tgcgcgcggg atcctcgacg ccgcgcgtgc gcagggccgg cgccaaaaaa
     8881 ccgaggttgg agcggctccc gcccggcacg cggccggcgg cgatgtgctc gagggcgccc
     8941 ggcagcgccg gcagccgtcg catgtgcacc gtcgccgcca gcgacgagcc gcggagaata
     9001 ttccgcaggt gcccgagcag accgaagccg gtgacatcgg tgcacgcgct gacaccgtgg
     9061 gcccgcgccg ccgcgagcgc ctggtcgttg agacaggtca tgctcgcgac cgccgcggcc
     9121 agctcggcgg ggctcgccag cccctgcttg atcgccgtgc cgaggacgcc ggtgccgagg
     9181 cccttggtga gcaccagcac ctgcccggcg cgcgccgttc ggttggacag cacatccttc
     9241 aaaaaaacgt cgccggtgac cgagaggccg aacttcggct ccgggtcgcg cacgctgtga
     9301 ccgccgacca ccggcgcgcc cgcgcgggcg caggcccggg ccccgccgcg caggatcgcg
     9361 tgcagcaccg acagcggcag gcggtcgtcg ggaaagaaca cgaggttgag gacgaagcgc
     9421 ggggtgccgc ccatcgcgta cacgtccgac agcgcgttga cggccgcgat ctccccgaag
     9481 atctccgggt cgtcgacgat cggcgcgatg acgtcggtcg tcagcaccag cccgcgctcg
     9541 tcgccatttt tgaaatcgag gcgcacgacg gcggcgtcgt cggcgaactc ggtcccgacc
     9601 accacgcccg tgggcgtctc actccgctga tcgggcggga gcacgccctc gagcacctgg
     9661 accaggtcgc cgagccgaag cttcgcggct caaccaccgc cgcgagcgag ctgcgtcagg
     9721 cgcgggtgca aggcgtcttc cgggaggtcc ttcatccgcg aagtctaccc cagcttgctg
     9781 ctacactggc cggcggatgg ccaggtcgac ccgcgcgctg gtcctcggta ccgccgggca
     9841 catcgaccac ggcaagacct cgctcgtcta tgccctcacc ggcatcgaca ccgaccgcct
     9901 ccccgaagaa aaacgccgcg ggatcaccat cgagctcggc ttcgccgcct gggagctcgg
     9961 cgggggcctg tccgccagca tcgtcgacgt ccccgggcac gaggccttcg tccgcacgat
    10021 ggtcgctggc gccggcggca tcgacctcgt gctgctcgtc gtctccgccg aggacggcgt
    10081 gatgccgcag acccgcgagc acctgcacgt gtgccagctg ctcggcctgc gccacggcgt
    10141 cgtcgcgctg accaagattg accgcctcgg caccgccaac gacgaccgcg ccgacgcgat
    10201 cgagatggcc gcggccgacg tgcgcgcggc cctggccgac ctcgccccca acctcgccga
    10261 cgccccgatc gtgccctgct ccgcccacac cggcgagggc ctgcctgcgc tcaccgccgc
    10321 gatccgccgc gtcgccgcca ccgtgccggc tcgcccggtc cgcgaccggc cgatcgtccc
    10381 gatcgatcgc gtgttcgccg tcaagggtca cggcaccgtc gccaccggca cgctgctgtc
    10441 cggccgcctc gacctcaccc gcgacgcccg actccagctg atcccgaccg gcgaacgggg
    10501 gccccgcgag ctgcgacccc gcgccctgca cgtccgcggc gtcgccagcg aacaggcccc
    10561 cgccggcagc cgcgtcgccc tcaacctcgg caacgtcggc accgacgacc tcgcccgcgg
    10621 cgacgtgatc tccagcggcc cccgcgtcgc ccgcggccag acctgcctcg ccgtcctcac
    10681 ccacctcgcc cacgacgacc tcccgtggcg cagcggcacc gccgtccagc tgtgcgccgg
    10741 cacggcctcg accaccgccc gcatcgaccc gctcgtgctc ctcggggaca tgtcggaaga
    10801 gacatccctg catgcgccgg tcgaagcgac ccgcccccct cctgacctgt ccccggggac
    10861 atcccccgcc acagctccgc ccggcgcccc tcccgacctg tcccaaagta catccacccg
    10921 cgtcaccgtc ccacccggcg cgaccgcgct cgtccgcctg cgcctcgacg ccccgctacc
    10981 cctgtggcac ggcttgccgc tgatcgtc
//
LOCUS       DASWNJ010001676         2359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_134776, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001676 DASWNJ010000000
VERSION     DASWNJ010001676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134776"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1087)
                     /locus_tag="VGB85_27835"
     CDS             complement(62..1087)
                     /locus_tag="VGB85_27835"
                     /inference="COORDINATES: protein motif:HMM:NF039450.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADYC domain-containing protein"
                     /protein_id="HEY0137940.1"
                     /translation="MRSVEANDTVLQGPGIVLQGPGIVLQGPGIVLQGPGIVLQGPGI
                     VLQGPGLVLQGVEFNGPGIVLQGPGIVLQGPGIVLQGPGIVLQGSTLSGTIVHEGVEY
                     PVSGLDFIGAEMELRLKAVVGGSEVTEVVIVRINDIKPSVAFDDVFMYDLTYRSEGSD
                     TWLPYCGKPGVAAVPMANYWDVETGDRVDDPNVVTFACADAALAKCALLGYRPWAEAT
                     NCEGKGKKQSCGEVSLQDHHQACTRMLRADYCGTGQSRTVNGTAIDVWDDLAPEIQTQ
                     FTDWPVEAEWTPEGASCVNFTRHPELGYPECFEKKNGKPLKFNKCGSFADPDSLLVNS
                     FSPIAPQ"
     gene            complement(1263..>2359)
                     /locus_tag="VGB85_27840"
     CDS             complement(1263..>2359)
                     /locus_tag="VGB85_27840"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY0137941.1"
                     /translation="YLGRWMTPGELRITSDPPGAAVTLERYVEAEGRLRAEAVSVLGV
                     TPVAPQTLPWGSYRLRFAAPGRTSVDVPVLLGRGESLDLGVVLPAAGTIPEGYIHVPA
                     GRFLYGSRDIESIRKLLGAEPMHTVETGPYLIARDEVTWADYIEFLEAQPPEQQAAIL
                     RASAAGLAHLEPHPAGGWRLSLTLGTRQHTLGPGAPLVYEGRTRRASVAWERLPVSAI
                     DERDAAAYIAWLDRSGRLPGARFCDEYEWERAARGADGRAFAVGDTLHPDDANFDMTY
                     GKLESAFGPDPVGSYLHALSPFGLRDTDGNVYELTVSPHKLGAIMVRGGAYFYAEIQA
                     RTAVRFPFPAGLRDPTVGLRVCATWPAPIP"
BASE COUNT          380 a          840 c          792 g          347 t
ORIGIN      
        1 cccgcggccc cgccgctttc agccccatcc ggggcatcag cggagcggcc gcgggagcgg
       61 gtcactgcgg tgcgatcggc gagaaggagt tgacgagcag cgagtccggg tcggcgaacg
      121 acccacactt gttgaacttg aggggcttgc cgttcttctt ctcgaagcac tcgggatacc
      181 cgagctcggg gtggcgcgtg aagttgacgc agctcgcacc ctcgggggtc cactcggcct
      241 cgaccggcca gtccgtgaac tgcgtctgga tctccggggc gaggtcgtcc cacacgtcga
      301 tcgcggtgcc gttgacggtg cgcgactggc cggtgccaca gtagtcggcg cgcagcatcc
      361 gcgtgcaggc ctggtggtgg tcctgcagcg agacctcgcc gcagctctgc ttcttgccct
      421 tgccctcgca gttggtcgcc tccgcccacg ggcggtagcc gagcagcgca cacttggcga
      481 gcgccgcgtc ggcgcaggcg aaggtgacga cgttcgggtc gtcgacgcgg tcgccggtct
      541 cgacgtccca gtagttcgcc atcgggaccg ccgcgacgcc cggcttcccg cagtagggca
      601 gccacgtgtc gctgccctcg gaccggtagg tcaggtcgta cataaagacg tcgtcgaacg
      661 cgacggaggg cttgatatcg ttgatccgca cgatgacgac ctcggtcacc tccgagccgc
      721 cgaccaccgc cttgaggcgc agctccatct ccgcgccgat gaagtcgagg ccgctgaccg
      781 ggtactcgac gccctcgtgg acgatcgtgc ccgacagcgt cgagccctgc agcacgatgc
      841 ccggaccttg cagcacgatg ccggggccct gcagcacgat cccggggccc tgaagcacga
      901 tcccggggcc gttgaactcg acgccctgca gcaccagacc cggcccttgc agcacgatgc
      961 cggggccctg aagcacgata ccggggccct gcagcacgat cccggggccc tgcagcacga
     1021 tgccggggcc ctgcagcacg atcccggggc cctgaagcac ggtgtcgttg gcctcgacgc
     1081 tccgcatcgt gacagcctcg ccagcatcct cggagccctc gcatgcagag ctcaacgcga
     1141 gcacagagac cagaaccgaa agattacgcc gcatagaccc aaccaccccc cacatggata
     1201 gtgaccgcga cgaggcgttc ggatccaaag aatttatgac gaatgcgaga tttattgttt
     1261 ttctatggga taggcgccgg ccatgtcgca catacacgta gcccgaccgt cggatcgcgt
     1321 agaccggcgg ggaacgggaa gcgcaccgcc gtccgcgcct ggatctccgc ataaaaatag
     1381 gccccgccgc ggaccatgat cgcgcccagc ttatgtgggg acactgtcag ttcatatacg
     1441 ttgccgtcgg tgtcacgcag cccgaacggg ctcagcgcat gcagatacga gccgaccggg
     1501 tcgggcccga acgcgctctc gagcttcccg taggtcatgt cgaagttggc gtcgtccggg
     1561 tgcagcgtgt cgccgaccgc gaaggcgcgg ccgtcggcgc ctcgcgccgc ccgctcccac
     1621 tcgtattcgt cgcaaaagcg cgcccccggc aggcgccccg agcgatcgag ccacgcgatg
     1681 taggcggccg cgtcgcgctc gtcgatcgcc gacaccggca ggcgctccca cgcgacgctg
     1741 gcccgccgcg tgcggccctc gtacaccagc ggggccccgg gcccgagcgt gtgttgccgg
     1801 gtgcccagcg tcagcgacag ccgccagccg cccgccggat gcggctcgag gtgcgcgaga
     1861 ccggcggccg aggctcgcag gatcgcggcc tgctgctcgg gcggctgggc ctcgaggaac
     1921 tcgatgtaat ccgcccacgt gacctcgtcg cgggcgatca ggtacgggcc ggtctcgacc
     1981 gtgtgcatcg gctccgcgcc gagcagcttg cggatcgact cgatgtcccg cgagccgtac
     2041 aggaacctcc ccgcgggcac atgaatgtac ccctccggaa tggtccccgc ggcgggcaag
     2101 acgacgccga ggtcgagaga ttcgccgcgt ccgagcaaca ccggaacatc gacgctcgtc
     2161 cgccccgggg ccgcgaagcg caggcgatag gagccccacg ggagcgtttg tggggccacc
     2221 ggagtgacac cgagcacgct cacggcctcg gcgcgcagcc ggccctcggc ctcgacatat
     2281 cgctcgagcg tgaccgcggc gccgggcggg tcgctggtga tccgcaattc ccccggggtc
     2341 atccagcgcc ccaggtagg
//
LOCUS       DASWNJ010001677         6582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_277953, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001677 DASWNJ010000000
VERSION     DASWNJ010001677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6582
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277953"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VGB85_27845"
     CDS             <1..404
                     /locus_tag="VGB85_27845"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HEY0137942.1"
                     /translation="FLWLPTLKRQSVVAGVISYADVRKLFDLANGVAPVVPQVLAHDA
                     ASLGAGAWKHYGPFTADAAGLKAVLTIAKGDADLYLRNGSKPTASAFDCRPFAEGLAS
                     ETCSLGAGTWYAAVNGYATTTDFTLDVTRGQ"
     gene            complement(710..1468)
                     /locus_tag="VGB85_27850"
     CDS             complement(710..1468)
                     /locus_tag="VGB85_27850"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0137943.1"
                     /translation="MQIVGRRDILGKLFREALPEVVSQGFVELLDRVYQTGEHYVGKE
                     VPALLQRSPGDSPELRYVDFVYQALRGPDGVVTGIIQQGIDVTDRKRAEQALRAGDER
                     LKLLVENIKDYAVVLTDQQGTVVEWQGGAERITGFSASEASGQKVDLIFTPEDLAAGR
                     PARERQLAAEDGRAESQQWHLKKDGSRFFTDTEGPEIYSGGSGMIDDIACFSGPGLGF
                     DLGLPEPGVTTYALDLTDECGASVHYDGKIVVSP"
     gene            complement(1402..2325)
                     /locus_tag="VGB85_27855"
     CDS             complement(1402..2325)
                     /locus_tag="VGB85_27855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0137944.1"
                     /translation="MRANSGEEALRKVLAGDFAAILLDVQMPGMSGFEVARLLRARPR
                     SRTTPIIFLTAGESREFPVEEAYGLGAVDYLMKPIVPAILRAKVEFFIDYFRQTRQLR
                     DFERRDVEATLREQRELWRTTLSCIGDAVIASDLERRVTFMNREAERLTGWAPGEAEG
                     RPLDEVFCIVNETTRERVPCPVRKVLDTGLVVGLANHTVLIARDGTEWPLDDSAAPIR
                     DGAGEIFGVVLVFREITERKQAEDERERLLREVETERERLADVFQWAPSFMASHGVPA
                     MSSNEPTTATCRSSAVATFSASCSARPYRRS"
     gene            complement(2383..3387)
                     /locus_tag="VGB85_27860"
     CDS             complement(2383..3387)
                     /locus_tag="VGB85_27860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137945.1"
                     /translation="MLDWLQNADSGLMLGALGFITGIASVIYARSQARGVHAQAEGIR
                     LQAVEALRAAELVAESARQQAVEALRAAGLVAESGRQQAVEALRAAGLVAESAKEQAA
                     EALRAAALVAESAKEQAMQALRAAELVAENARQQAAEAVRAAELVAENARQQALEALR
                     AAELVAEGARQQAIEALRAAGLVAQSAKDQATEALRAADLLADSAVSARVREMRSRNF
                     RANPNLPTGIQEIIKSVGGPDAYSVMLDSMDIAQEIYVLRKRGLVSDENWRRWMNDQM
                     VLISQSPVFKDIFRRDADQGALFPEFVSAFQPVFEGRGIADPTKDPSPDDPDPTTANR
                     "
     gene            complement(3904..4911)
                     /locus_tag="VGB85_27865"
     CDS             complement(3904..4911)
                     /locus_tag="VGB85_27865"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY0137946.1"
                     /translation="MRRHSRRAAQQTPPDAAPGGECDTGDAPGHPLHAAESILRLSRS
                     RSAGGGRSHMVAGPAAAASADGRAPDPRRAPLSERPKEDPMTYHHRTRSLPALAATAL
                     AVVMLAGCRKECPIDEATPAAASIEDSAQEDSAQEEVTMDIQRYLPWRKRDTSETKQQ
                     QDQVAERDDAEKQDRLATQDSRDDVVRPGHSWAPFLPFHHVDSLFDSLWRDFGDFPTM
                     PGFLSGLDMPRFDLSERDDRYVVTADLPGLSEKDIDVSVSNGVLTVRGERKEERKEDA
                     KGGYRMQRSYGAFHRSMTLPGDALVDGVDATYKNGVLTVELPRSKERRPGARNIEVKA
                     G"
     gene            5005..5526
                     /locus_tag="VGB85_27870"
     CDS             5005..5526
                     /locus_tag="VGB85_27870"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEY0137947.1"
                     /translation="MSQLIVVGYPDLTRAQQVLATVWRMEKEHLVDLEDAAIVTRTEK
                     GRVRLIQTQDVAASGIVGGSFLGLMIGLLFLNPILGLVVGSASGALAGWMSDVGVSDD
                     FMRELGKQLAPGSAALFILVRRGAPDRIVEELAPHGGTLLRTNLSRHDENELRALIER
                     IQAEARGDGPPPR"
     gene            5593..>6582
                     /locus_tag="VGB85_27875"
     CDS             5593..>6582
                     /locus_tag="VGB85_27875"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY0137948.1"
                     /translation="MPEHLRHLLDDTWHGITGLRWLVAAAIVVIGTGLVTLIRSGLLG
                     HLRAVAARSKTDLDDFFVSLAERTGVWFVALLMLRSATLVLELSDRWDERAQMLLIVG
                     GMIQGGLWGSALVLYLVDRRFARHVGSGRVEPHVMPVGQTLLRSAGLVLVWTIVLMTV
                     LSSFGIDISGLVTGLGIGGVAVALAVQKILGDVLASILIAVDRPFSPGDYIALGTQQG
                     TVLRIGIRSTVIASLGGEELIIANNDLLSARVQNYTRMTERRVSFKVGLVYDTTPEQL
                     DALPALIKELLADRGQVRFERGAVTNLADSWIEVEVAYWVLDPSYDLFVAIHQA"
BASE COUNT          990 a         2336 c         2139 g         1117 t
ORIGIN      
        1 acttcctgtg gctgccgacc ctcaagcgcc agagcgtcgt cgccggcgtg atcagctacg
       61 ccgacgtgcg caagctgttc gacctcgcca acggcgtcgc accggtcgtc ccgcaggtgc
      121 tcgcgcacga cgccgccagc ctcggggccg gcgcgtggaa gcactacggc ccgttcaccg
      181 ccgacgccgc gggcctcaag gccgtgctga cgatcgccaa gggcgacgcc gacctgtacc
      241 tgcgcaacgg cagcaagccg accgccagcg ccttcgactg ccgccccttc gcggagggcc
      301 tcgccagcga gacctgctcg ctcggcgccg gcacctggta cgccgccgtc aacggctacg
      361 cgaccacgac cgacttcacg ctcgacgtga cccgcggcca gtgacctacc ggccgcgacc
      421 gcggctccgc gagccgaagc gaggccctgg tccgacttcg gctcgcatga actaatcagc
      481 acgcggattc accgcggacg cctcacaggc cgaattgcgc gaaacgaggc tctccctgtc
      541 gggtaagccg acggtcacgc cgtcgaagtc tgcccacgaa tagctgcacg gctgccgtac
      601 tcgactcgcg ggccatcgga ccggtcctgc gtgcacaggg tgtctccggg ccatacaggt
      661 cgcagcgctc tccgaccggg cagtcctggg cgaatgggtc gcaggtcgat cacgggctga
      721 cgacgatctt tccgtcgtag tggaccgagg cgccgcattc gtccgtcagg tcgagggcgt
      781 aggtcgtgac gcccggctcg gggagcccga ggtcgaaccc gagtcccggg ccgctgaagc
      841 acgcgatatc gtcgatcatg ccggacccac cgctgtagat ctccggcccc tccgtgtcgg
      901 tgaagaaacg ggagccatct ttcttgaggt gccattgctg gctttcggcg cggccgtcct
      961 cggctgcaag ctgcctctcc cgcgcagggc gccccgccgc cagatcctcg ggcgtgaaga
     1021 tgaggtcgac cttctgcccg ctcgcttcac tggcagagaa gcctgtgatc cgctcggcgc
     1081 ctccctgcca ctcaaccacg gtgccctgct ggtccgtgag cacgacggcg tagtccttga
     1141 tgttctcgac caggagcttg agccgctcgt cccctgcgcg taatgcctgc tcggcccgct
     1201 tccgatcggt gacatcgatc ccctgctgta tgatcccggt caccaccccg tccgggccgc
     1261 ggagggcctg gtatacgaag tcgacatacc ggagctccgg cgagtcgccc ggggatcgct
     1321 ggagcagcgc cggaacttct ttgccgacgt agtgctctcc ggtctggtac acccgatcga
     1381 gcagctccac gaagccctgg ctcacgacct ccggtagggc ctcgcggaac agcttgccga
     1441 gaatgtcgcg acggccgacg atctgcaggt agcggtcgtt ggctcgttcg aagacatggc
     1501 cgggaccccg tgtgacgcca tgaatgacgg cgcccactgg aacacgtcgg ccagtcgctc
     1561 ccgctcggtc tcgacctcgc ggagcagccg ctctcgctcg tcttccgcct gcttgcgctc
     1621 ggtgatctcg cgaaacacga gcaccacgcc gaagatctcg ccggccccgt cccgaatggg
     1681 agcggccgag tcgtcgagcg gccactcggt tccgtcgcgg gcgatcagga ccgtgtggtt
     1741 tgcgaggccg acgaccaggc cagtgtccag caccttgcgg accggacatg gtacgcgctc
     1801 ccgggtcgtc tcgttgacga tgcagaacac ctcatcgaga gggcgcccct cggcctcgcc
     1861 tggcgcccaa cccgtcagac gctcggcctc gcggttcatg aaggtgacgc gcctctcgag
     1921 atcggacgca atgaccgcgt caccgatgca tgacagggtc gtccgccaca gctcgcgctg
     1981 ctcccgaagg gttgcctcga cgtcccttcg ctcgaagtcc cggagctgtc gcgtctgccg
     2041 aaaatagtcg atgaagaatt cgaccttggc gcgcaagatc gcagggacga tcggtttcat
     2101 caggtagtcc accgcgccga gaccgtaggc ttcctccaca gggaactctc tactctcgcc
     2161 ggcggttagg aagatgattg gagtggtcct cgaccgcggc cgcgctcgga gcaacctcgc
     2221 cacctcaaac ccgctcattc ccggcatctg tacgtcgagc aagatggcgg cgaagtcacc
     2281 cgcgagcacc ttgcgaagcg cctcctcgcc cgagttcgct cgcaccagcg tcaggtctcc
     2341 gtcagctcgg cgaggacccc ggcggcgggt cctggtttgt cgtcacctgt tcgctgtcgt
     2401 cggatcggga tcgtcggggc tcgggtcctt ggtcgggtcc gcgatgccgc ggccctcgaa
     2461 gaccggctga aaggcagaga cgaattcagg gaacaaggcg ccctggtcgg cgtcgcgccg
     2521 gaagatgtcc ttgaagacgg gcgactgcga gatcagcacc atttggtcgt tcatccagcg
     2581 tcgccagttc tcgtcggaga cgagaccacg cttacgcagg acgtagatct cctgggcgat
     2641 gtccatcgaa tcgagcatga cgctgtaggc atcggggccg ccgacgctct tgatgatctc
     2701 ctggatgccg gtaggcaaat tcgggttggc gcgaaagtta cgcgaacgca tctcacgtac
     2761 gcgggcggac accgccgaat ccgccagcag gtccgcggcg cgcaaggcct ccgtcgcctg
     2821 atcttttgcg ctctgcgcca ccaggcccgc cgcacgcaag gcctcgatgg cctgctgcct
     2881 ggcaccctcg gccaccagct cggccgcacg cagggcttcg agcgcctgct gccgggcgtt
     2941 ctcggcgacg agctccgcgg cgcgtacggc ctcggccgcc tgctgccggg cgttctcggc
     3001 gacgagctcc gcggcgcgta gcgcttgcat ggcctgctcc ttcgcgctct cggccaccag
     3061 agccgcggcg cgtagcgcct cggccgcctg ctccttcgcg ctctcggcga cgaggcctgc
     3121 ggcgcggagc gcctcgacgg cctgctgccg tccgctctcg gcgacgagac ctgcagcgcg
     3181 cagggcttcc acggcctgct gtcgggcgct ctcggcgacg agctcggcgg cgcgcaaggc
     3241 ctcgacggct tgcagacgga tcccctcggc ttgcgcatgc acgccgcggg cctggctgcg
     3301 cgcgtagatg accgaggcga tccctgtgat gaagccgagg gctccaagca tcaagcccga
     3361 gtcggcgttc tgaagccagt caagcatggg gtgacaacga tgaacgagtt cgggctgctc
     3421 cgcccgggtc ggtccagggt gcgaatttgg tcgagggcat cggcacgtcg tacgggatcc
     3481 tgtgaggccc gcgccgcgga tatttcccac ccggcgcagc cccgaggacg aacccagcga
     3541 tgcccgccca tgacgaagcg cacgcgatcg ggagcagaaa agagcacgtc cgcgacgacc
     3601 cattccgagt tgagaggccg aagctcgcgc tgctgcggag gccaaggtcc gcgccgcaaa
     3661 gcgctgtgga cggcctctct tcgttccgcg gctacctccg ggcgcgcatc accagaccga
     3721 cggtccgcgc aatcgaggcc gaacacaatc tttcttccga cgctctactc tttcgcggcg
     3781 gtcagtccca tttcagggcc cgaaaaaacc ggcgcggcgg ccacgaagct cggccaccgc
     3841 gccggttcac atcggccgcg gatcgccgcg ctacggcgga tccgctgcct ccatcatgcg
     3901 gcctcacccg gccttcacct cgatgttgcg cgcaccgggg cgccgctcct tggacctcgg
     3961 gagctcgacc gtcagcacgc cgttcttgta ggtggcatcg acgccgtcca cgagggcgtc
     4021 gcccggcagc gtcatgctgc gatggaacgc gccgtagctg cgctgcatcc ggtagccacc
     4081 cttggcgtcc tccttgcgct cctccttgcg ctcgccgcgg accgtcagca cgccgttgct
     4141 cacggacaca tcgatgtcct tctcgctgag ccccggcaga tccgcggtga cgacataacg
     4201 gtcatcacgc tcgctcaggt cgaagcgcgg catgtcgagg cccgagagga accccggcat
     4261 cgtcgggaag tcgccgaagt cgcgccagag gctgtcgaac aggctgtcca catgatgaaa
     4321 tggaagaaat ggagcccacg aatgccccgg gcggacaacg tcgtcgcggc tgtcctgggt
     4381 cgcgagtcgg tcctgcttct cggcatcgtc acgctccgcg acctggtcct gctgctgctt
     4441 cgtctcgctc gtgtcgcgct ttcgccacgg caaatatcgc tggatatcca tggtaacctc
     4501 ctcctgcgct gaatcttcct gcgctgaatc ttcaatggac gcggccgctg gcgtcgcctc
     4561 gtcgatcgga cattctttgc gacagcccgc cagcatcacg accgccaggg cggtcgccgc
     4621 cagcgccggc aggctgcggg tgcgatggtg gtaagtcatg ggatcctcct ttggtcgctc
     4681 cgacagcggc gcgcggcgag gatccggcgc acggccgtcg gcgctcgcgg ctgcggcggg
     4741 gccggccacc atatggctgc ggcctccacc cgcgctccgc gaccgcgata gtcttaagat
     4801 cgactccgcg gcatgcaagg ggtggcccgg agcgtctcct gtgtcgcatt caccgccagg
     4861 ggccgcatca ggcggcgtct gctgcgcggc ccgacggctg tggcggcgca ttcgctggaa
     4921 gaccgcgtgc gccgggcgct ctgctggcgc cgcccgggtc gctcggcaga ttcggggcga
     4981 ccccacgttc gcggagaacc agccatgagt cagctcatcg tcgtcggata tcctgacctc
     5041 acccgggccc agcaggtcct cgcgaccgtg tggcgcatgg agaaggagca cctggtcgac
     5101 ctcgaggacg ccgccatcgt cacgcgcacc gagaagggcc gcgtgcgcct gatccagacc
     5161 caggacgtcg cggcctccgg gatcgtcggc gggagcttcc tcgggctgat gatcgggctg
     5221 ctgttcttga acccgatcct cggcctcgtc gtcggctcgg cctccggggc cctcgccggc
     5281 tggatgagcg acgtcggcgt ctccgacgac ttcatgcgcg agctcgggaa gcagctcgca
     5341 cccggcagcg ccgccctgtt catcctggtc cgtcgcggcg cgcccgaccg catcgtcgag
     5401 gagctcgcgc cgcacggcgg caccctgctg cgtaccaacc tctcgcgcca tgacgagaac
     5461 gagctgcgtg ccctgatcga gcgcatccag gccgaagcac gcggcgacgg ccctccgccg
     5521 cggtagcacc gggcttacag ccccgcgccg tctctggcgc ctggcggatc ttgcgctatg
     5581 ggtctggcgc acatgcctga gcacctgcga cacctcctcg acgacacgtg gcacgggatc
     5641 acaggtcttc gctggctcgt cgccgccgcg atcgtcgtca tcggtaccgg gctcgtgacg
     5701 ctgatccgct cgggattact cggccatctg cgcgcggtcg cggctcgcag caagacggac
     5761 ctcgacgact tcttcgtctc cttggccgag cgcaccgggg tgtggttcgt ggcgctgctg
     5821 atgctgcgca gcgcgaccct cgtgctcgag ctgtcggatc gctgggacga gcgcgcgcag
     5881 atgctgctga tcgtgggagg catgatccag ggcggcctgt ggggcagcgc gctggtcctc
     5941 tatctggtcg atcgccggtt cgcccgtcac gtcggttcgg gcagagtcga gccgcacgtc
     6001 atgccagtcg gtcagacgtt gctgcgatcc gccggcctcg tcctcgtgtg gacgatcgtg
     6061 ctgatgacag tcctgtcgtc gttcggcatc gacatcagcg ggctggtcac gggcctcggg
     6121 atcggtggtg tcgcggtcgc gctcgccgtc cagaagatcc tcggcgacgt gctcgcgtcg
     6181 atcctgatcg ccgtcgaccg gccgttctcg cccggcgact acatcgcgct cggcacccag
     6241 cagggcacgg tgctgcggat cggcatccgc agcaccgtga tcgccagcct cggcggcgag
     6301 gagctcatca tcgccaacaa tgacctgtta tcggcgcggg tccaaaatta tactcggatg
     6361 accgaacgtc gcgtctcatt caaggtcggc ctcgtctacg acacgacccc ggagcaactc
     6421 gacgcgctcc ccgcgttgat caaggagctg ctcgcagacc gcggccaggt ccgcttcgag
     6481 cgcggcgccg tgaccaacct cgcggactcg tggatcgagg tcgaggtcgc ctattgggtg
     6541 ctcgatccga gctacgacct gttcgtcgcc atccaccagg ca
//
LOCUS       DASWNJ010001678         8584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_30011, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001678 DASWNJ010000000
VERSION     DASWNJ010001678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 8584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8584
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30011"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5697)
                     /locus_tag="VGB85_27880"
     CDS             complement(<1..5697)
                     /locus_tag="VGB85_27880"
                     /inference="COORDINATES: protein motif:HMM:NF015497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HEY0137949.1"
                     /translation="MRDLRDPRNADSSQAEPRSAAGAVSPQGPDKHTKGGAKEEKKSP
                     FLDRSQMTPAIEFPKGGGSVRGMGEKFTPSPFTGSGAFAVPVALSPGRQGFGPTMGLQ
                     YSSGGSNSPYGFGWALDLPAISRKTDKGLPRYVDSSESDTFILSGAEDLVPVLRPENN
                     WIAGGSVADRQVIVRDGFRVFAYRPRVEGLFARIERWVANDTGEVHWRSISRENVTSY
                     YGRSAESRIADPANPEHVFQWLLDESRDDRGNIAVYQYKAEDLAGVTTWAPSEHNRRE
                     PGVSAQAQRYLKRVLYGNAAPSVASDWRFEVVLDYGEHGTEVDGELEVSPEEVRAWPV
                     RDDAFSSFRPGFDLRTYRLCRRVLMFHNFEALGAEPYLVAATVLTHDATPAASYVREV
                     QHVAYERNPITERFTPAAFPAVHFTYSPAVVDQEIRSLDAGSLADVGSAIDGGLYRFI
                     DLDGEGLPGILSEQAGHWYYKRNLGDGRFGPLEALHELPSTADLAGGRQQLTDLDGAG
                     VKHLVSFGPPTTGFYERTAAAGFSEFRYFRTVPQIDWNDPNLQFVDLSGDGLPDVLLS
                     RDDHFVWYRSLGKEGFEAPCVLRKPHDERSGPALVFADGTGSVFLADMTGDGLADIVR
                     VRLGEVAYWPNHGHGRFGRKITLGDSPVFDLNDQFEPARLRLADVDGTGPTDLIYLGA
                     AGTRIWMNQSGNRFAPAISVVGFPLLDDAASVDVVDLRGNGTACLVWSAGRGTELQRL
                     HFIDLMKGQKPHLLVEIDNGRGLRTRARYAPSTRSYLRDRLAGRPWATRLSFPVQVVE
                     CVESFDQIARQKFVQHFAHHHGYFDAYEREFRGFGMVEQWDTESYEDFAGESLFSFDF
                     EAVEEKLHQPPVHTKTWFHPGAFLGLEHVTQILKSEYYQGDADAKQIPDTQWPAELTP
                     EEIRETTRALAGRALHTEVYALDGTAAAVHPYTVTDANFQIQLLQPRAKLPHAVLYVH
                     DRESIVHHYERSPADPRVAHSFVITVDAFGLVTRSASVTYPRRTPAFDEQGMLTVVLA
                     DATFAHLIGEADDYRVGNPLTTRAFELLGLTAPGVSDALALSAIADAAEEATEVPFEG
                     PLGGDPLKKRLLSDTRVRYWADDLSGPLAYGVPGKRALVYDTDAKAMSAGQFTTVFGG
                     LAQAPDATVMANEGGYVLDGGAYWMRSGRQVHAPAKFYQPVEFLDPFGNSTTVTYDDD
                     VLFMLEVEDPLGNTVTAEVDRRVLAPWKMTDPNGNRVAVAFDTRGVVTKSAVMGKAGA
                     NEGDTLEAPTSTFEYDLFAWQTQQKPLHVKTRARETHADVNTRWLESYAYFDGAGRTR
                     MTKVQAAPGLAPARDGNGELVLDGGELVLVDTFPAVRWIGTGRTVVDNKGNPVKQYEP
                     YFSSTPDYEDEAELVEQGVTPVLHYDPLGRLVRAELPNGTESRVEFDPWMRLNYDPND
                     LVEGSEWYESRIGYAGADAGLLSERTAAIQAYAHRNTPTTANLDSQGRSFRVSQHNID
                     IVTNLAVFNVTKSVFDVQGNVLDVLYYVNVNLERSAQQTEYGLLGQPLRTFSTDAGNR
                     VMLASVLGEPIRAWNHRGFITRVVYDVARRPTQQWVKPGASAEQLVTFNVYGEQVPDS
                     EDNNLRGQLYRSYDSAGALTGELFDFKGSLLRQSRRMAADYKVTPDWADLAAEATLVD
                     IETAAAALLESETFTTKTTYDALDRVLTQMTPDESETAHRYDEGGRLRAVESKLRGAA
                     TATEFVEQIEYDAKGQRLYIELGNGTTTAYSYDPKTSRITRIRTDRASDSAVMQDLKY
                     TYDPVGNITAIRDDAQQTVYFSNAVVSPTQTYVYDAVYRLVKAHGRETPSGQPQHEQL
                     VPSPSPPPNDGSAVILYTETYLHDEVGNILEIVHSAGVNSWTRHYKYAADSNRLLAT"
     gene            6021..>8584
                     /locus_tag="VGB85_27885"
     CDS             6021..>8584
                     /locus_tag="VGB85_27885"
                     /inference="COORDINATES: protein motif:HMM:NF015501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tc toxin subunit A"
                     /protein_id="HEY0137950.1"
                     /translation="MPTLIVKGKVVTVDGEPLERKIVEVRPRLSAAVYGTAATTLTDG
                     GNYMATLSTTTAGSGSTFDVEVVVCELDNTEIGRSVLLYDVAGEITVDIVVGAADYRG
                     RSDFRRIEARIDPLRGSLAVDELGSEHIEYLSAKLDMDPLRIAQYIQAHRMSVLLDTV
                     PAWAFYALLRQGLPAEVPQLLAQGRPVHEGGLAAAIAGNVIADPGEPTLNAVLDELQA
                     QAAESAVWAHPTDPDAKSKFRRLLDTAAGPEASYTVQLAFMEKHLAHTGTLAEFWDAV
                     DADIDLGPTVRATYEWTLQINTLANEHMPLITALQAKRADIGDPIKSFRDLSALDVAD
                     WVDLLSSGIGAPASIPLDWPEEDRILRYAETIERIVTDTYPTTVIHRRIVRDHTASPG
                     SIAGAPDLIQFFDDNPAFELSPHELDAYLADHPTALDNVTDAAATRANLKKLQRLARL
                     APRGFQYRVLKPLYQAGLHSALQIGRMTEKSFVRKYGTNLGGDDIARSVYQRARMSAG
                     IALMVATQFARGFNKTPISVVPSSTLPDYEGHPDLEALFGNLDFCACDHCRSVYSATA
                     YFVDLLQFLKDQPGKDAGSNALDALMERRPELTAIELSCINTNTEVPYIDLVNELLEY
                     HVSRHANAPLAVSSSFWQTTWTAEELAVRPEHQLSQAYNVALDTPYPFGLPFDLAFAE
                     TSLYHQSFDVSRERMENAYPHNDPDYADRRARARLGLDLMSWYVIINSGLISWSPRAF
                     WGGETADEPQPPDWWTPLRTVSTFLKRAEIDISELAALLRTRFIGRAGLSISYAAPPD
                     DCNLDEAEILTLGEADLGKIHRFIRLQRKIGWTADELDLALRTLSGDPPALDGASVR"
BASE COUNT         1511 a         2832 c         2675 g         1566 t
ORIGIN      
        1 cgtggccaga agccgattac tgtccgcagc atacttgtaa tgacgcgtcc agctatttac
       61 ccccgcagaa tgcacgatct cgaggatgtt tccgacctca tcgtgcagat atgtctcggt
      121 gtacaggatg accgcactcc cgtcattagg aggggggctc gggctcggga ccaactgctc
      181 atgctggggc tgtccgctcg gagtctcgcg gccgtgcgct ttgaccaagc gatagaccgc
      241 gtcgtacacg tacgtctggg tcgggctcac caccgcgttg ctgaagtaca ccgtctgctg
      301 agcgtcgtcg cggatcgcag tgatgttgcc cacgggatca taggtgtact tcagatcctg
      361 catcactgcc gagtcgctag cccggtcggt cctgatccgg gtgatgcggg aggtcttggg
      421 gtcataagag taggcagtcg tggtaccgtt gccgagctcg atgtacaggc gctgtccctt
      481 ggcgtcgtac tcgatctgct cgacgaactc ggttgccgtc gcggcgccac gcagcttgct
      541 ctccacggcc ctcagccgac cgccctcgtc atacctatgg gcggtctcgc tctcgtccgg
      601 ggtcatctga gtcaggaccc ggtcgagggc gtcgtacgtg gtcttggtcg tgaaggtctc
      661 gctctcgagc aatgcggcgg cggcggtttc gatgtcaacc agggtcgcct cagccgcgag
      721 gtcagcccag tcgggcgtga ccttgtagtc cgccgccatc cgccggctct gccggagcag
      781 actacccttg aagtcaaaca gctcgcccgt cagcgcgccg gcgctgtcgt agctgcgata
      841 gagctggccg cggaggttgt tgtcctccga gtcggggacc tgctcgccgt acacgttgaa
      901 ggtgacgagt tgctcggcgc tggcgccggg ctttacccac tgctgggtcg ggcgccgtgc
      961 gacgtcatag acgacccggg tgatgaaccc tcgatggttc caggcacgga tcggctcccc
     1021 gagcactgaa gcgagcatga cccggttgcc ggcgtctgtc gagaaagtcc tcaggggctg
     1081 cccgagcagc ccgtactctg tctgctgggc gctgcgctcc aagttcacgt tcacgtagta
     1141 cagaacgtcg aggacgttgc cctggacgtc gaacaccgac ttggtgacgt tgaagaccgc
     1201 cagattggtg acaatgtcga tgttgtgctg cgagacgcgg aaggagcgtc cttgactgtc
     1261 gaggttcgct gtcgtcgggg tgttccggtg ggcgtacgct tggatggcag cagtcctctc
     1321 ggagagcagg ccggcatctg cgcccgcgta accgatgcgg ctctcgtacc actccgaccc
     1381 ctcgaccaag tcgttcggat cgtagtttaa tcgcatccac gggtcgaact cgacccgcga
     1441 ctcggtgccg ttcggtagct cggcgcgcac caagcgcccg agcgggtcgt agtgtaggac
     1501 cggcgtcacg ccctgctcga ccaactcggc ctcgtcctcg tagtcgggcg tgcttgagaa
     1561 gtacggctcg tactgcttga cggggttgcc cttgttgtcg accaccgtgc ggccggtgcc
     1621 gatccagcgg accgccggga acgtgtcgac gagcacgagc tcaccgccgt ccagcacgag
     1681 ctcgccgttc ccgtcccgtg cgggtgcgag gcccggggcc gcctgcacct tggtcatccg
     1741 cgtgcggccc gcaccgtcga aataggcgta actctccagc cagcgggtgt tcacgtcagc
     1801 atgggtctcg cgtgcccgtg tcttgacgtg gagcggcttc tgctgcgtct gccacgcgaa
     1861 caggtcgtac tcaaaggtac tggtcggcgc ctcgagggtg tcgccctcgt tcgcgccagc
     1921 cttgcccatc acggcagatt tggtcacgac gccccgcgtg tcgaacgcca ccgcgacccg
     1981 attgccgttg ggatccgtca tcttccacgg tgcgagcacg cggcgatcga cctcggcggt
     2041 gactgtgttg ccgagcgggt cctcgacctc cagcatgaac agcacatcgt cgtcgtaggt
     2101 gacggtggtc gagttgccga aggggtcgag gaactcgacc ggctgataga acttggccgg
     2161 cgcatgcacc tgacgacccg agcgcatcca gtaggcgccg ccgtcgagta cgtaaccgcc
     2221 ctcgttcgcc atcaccgtcg cgtccggcgc ctgcgcgaga ccaccgaaca cggtggtgaa
     2281 ttggcccgcg gacatcgcct tggcgtccgt gtcgtagacc agcgcacgct tcccgggcac
     2341 accgtacgcg agcgggcccg acaggtcgtc cgcccagtag cggacccgcg tgtccgagag
     2401 cagacgcttc ttcaacgggt cccccccaag cggcccctcg aagggcacct cggtcgcctc
     2461 ctcggcggcg tcggcgatcg cactcaacgc caaggcatcg ctgacgccag gagccgtgag
     2521 gcccagcaac tcaaaggccc gcgtggtcag cgggtttccg acgcggtagt cgtccgcctc
     2581 cccgatgagg tgcgcgaagg tggcgtccgc gagaacgact gtcaacatgc cctgttcgtc
     2641 gaacgctggc gtcctccggg ggtaggtgac actcgcgctg cgagtcacca ggccgaaggc
     2701 atccacggtg atcacgaagc tgtgggccac ccgcgggtcc gccgggcttc gctcgtaatg
     2761 gtgcacgatc gactcacggt catgcacata cagcacagcg tgcggaagct tcgctcgcgg
     2821 ctgtagcagt tggatctgaa agttggcatc ggtcaccgtg tacgggtgga ccgccgccgc
     2881 ggtgccgtcg agtgcgtaga cctcggtatg cagcgcccgt cccgcgagcg cccgcgtcgt
     2941 ctcgcggatc tcctcgggcg tgagctcggc cggccattgc gtgtccggga tctgcttcgc
     3001 atccgcgtcg ccttggtaat actccgactt caagatctgg gtgacgtgct ccaagccgag
     3061 gaacgccccc gggtggaacc acgttttggt gtgcaccggc ggctggtgga gtttctcctc
     3121 gaccgcctca aaatcgaacg agaacagact ctcgcccgcg aaatcttcgt aagactcggt
     3181 atcccattgc tcgaccatac cgaagccgcg gaactcgcgc tcgtaggcgt cgaagtaacc
     3241 atggtggtga gcgaagtgct ggacgaattt ctgccgggct atttgatcaa agctctcgac
     3301 gcactcgacc acctgtaccg ggaacgacag ccgtgtcgcc cacgggcggc cggcgaggcg
     3361 gtcgcgcaga tagctgcgcg tggagggcgc gtagcgggcg cgggtccgca ggccgcggcc
     3421 attgtcgatc tcgacgagca gatgcggctt ttgtcccttc atcaggtcga tgaagtgcag
     3481 acgctgtagt tcggtgccgc gccctgcgga ccatacgagg caggcggtcc cgttgccacg
     3541 caggtcgacg acgtcgacgc tggccgcatc atccagcagg gggaagccga cgacggagat
     3601 cgctggggcg aagcgatttc ccgactggtt catccagatc cgcgttcccg ccgcgccgag
     3661 gtagatcagg tctgtgggtc cagtcccgtc gacgtcggcg agacgcaggc gggccggctc
     3721 gaactggtcg ttcaggtcga acacagggct atccccgagc gtgatcttgc ggccgaagcg
     3781 gccatgcccg tggttcggcc agtaggcgac ctcccccagc cgcacgcgga cgatgtcggc
     3841 caaaccgtcg cccgtcatgt cggcgaggaa cactgagccg gtcccgtcgg cgaacacgag
     3901 cgccggcccc gagcgctcgt catgcggctt acgaaggacg cagggggcct cgaagccttc
     3961 cttccctagc gaccggtacc atacgaagtg gtcatctcgg gagagcagca cgtctggtag
     4021 accatctccg ctgaggtcga cgaactgcag gttcggatcg ttccagtcga tctgcggcac
     4081 agttcggaag tagcggaact cgctgaagcc agcagccgcg gtcctctcgt agaaccccgt
     4141 cgtaggcggg ccgaagctta cgaggtgctt tacccccgcg ccgtccaggt ccgtgagctg
     4201 ctggcgcccc ccggcgagat ccgcagtgga cgggagttcg tgcagcgcct cgagaggccc
     4261 aaagcgcccg tctccgaggt tgcgcttgta ataccagtgc cctgcctgct cgctgaggat
     4321 ccccggcaac ccctccccgt cgaggtcgat gaagcggtag aggccgccgt cgatcgcaga
     4381 gccgacgtcc gcgagcgacc cagcgtcgag cgagcggatc tcctgatcga ccaccgccgg
     4441 actgtaggtg aaatgcaccg ccggaaacgc cgctggcgtg aaacgctcag tgatcggatt
     4501 tcgctcatag gctacgtgct gcacctcgcg cacatagctc gccgctggcg tggcatcgtg
     4561 ggtaagcacg gtcgccgcga ccaggtacgg ctccgccccc agcgcctcga aattgtgaaa
     4621 catcagaacg cggcggcaca gccggtaggt acgcagatcg aagcccggtc ggaagctcga
     4681 gaacgcatcg tctcggacgg gccaggcgcg tacctcctcg ggcgagactt ccagctcgcc
     4741 gtcgacctcg gtcccgtgct caccgtagtc gagcacgacc tcgaagcgcc agtcactggc
     4801 gaccgacggg gcggcattgc cataaaggac ccgcttgagg tatcgctggg cctgggcgct
     4861 cacgccaggt tcccggcggt tgtgctcgga tggcgcccaa gtcgtcaccc cggcaagatc
     4921 ttctgcctta tattgataga cggcgatgtt gccgcggtcg tcgcgcgact cgtcgagaag
     4981 ccactgaaac acgtgctccg ggttggcggg gtcggcgata cgcgactcgg ccgagcggcc
     5041 gtaataggag gtgacgttct cgcgtgatat cgaacgccag tgcacctcgc ccgtgtcatt
     5101 ggcaacccac cgttcaatcc gcgcgaaaag cccctccacc cggggccggt aggcaaagac
     5161 cctgaagccg tcccggacga tgacttggcg atccgcgacg cttccgcccg cgatccagtt
     5221 gttctccggg cgcaacacgg gtacgaggtc ctccgccccc gagaggatga atgtgtcgga
     5281 ctcgctacta tcgacgtagc gaggaaggcc cttgtcggtc ttccggctta tcgccgggag
     5341 atcaagtgcc cagccgaacc cgtacggcga attgctgccg ccggagctgt actgcaggcc
     5401 catcgtgggt ccaaaacctt ggcggccggg ggatagcgcg acggggaccg cgaaggcccc
     5461 agagcccgtg aacggactcg gggtgaactt ttctcccatg ccacgtaccg acccacctcc
     5521 tttaggaaac tcgatggccg gcgtcatctg cgagcggtcg aggaacggac tcttcttctc
     5581 ctctttcgct cccccctttg tgtgcttgtc cggcccttgg ggggagactg cacccgcggc
     5641 tgaacgtggc tctgcctggc tcgaatcagc gttccgggga tcacgtagat cgcgcataga
     5701 agtgcccttc ctcaggaggg cacaccgccg acctgaccca tcaagcaata atgaacgttc
     5761 aggcccgcgt caggccgcca gcatgagtcg cggactatag ctcagccaac gcgcgtgtct
     5821 agaaaaatat cgcggcgcga atagtcgata ctgggcctgg acaatttcga ccattcccct
     5881 cggccgatgt acgtggcgcg ccaaaacgta tctcactttt taagttgcct ccgcagacgc
     5941 cctcgactag cgtgcttcct cattcagtca tgatgtgaat gagtggcctg cgccatgcga
     6001 gcgcggtagg agatatttgt atgccgacac tgatcgtcaa gggtaaagtt gtcaccgtcg
     6061 atggagagcc cctggagcgg aagatcgtcg aggtccggcc gcggctcagc gcggccgtct
     6121 atggcacggc agcgacaacg ctgactgacg gcggcaacta catggcgacg ctgtccacta
     6181 ccacggcggg aagcggtagc acctttgacg tcgaagtcgt cgtctgcgaa ctcgacaaca
     6241 cggagatcgg gcgttccgtg ctcttgtacg acgtggcggg cgagatcacg gtcgatatcg
     6301 tagtaggcgc agctgactac cgtggacgtt ccgattttcg ccgtatcgag gctcggattg
     6361 atccacttcg aggcagcctc gcggtggacg aactcggaag cgagcatatt gagtatctat
     6421 cggccaagct ggacatggat cctctgagga ttgcgcagta catccaggcg catcgcatga
     6481 gcgtgctttt ggatacggta ccggcctggg cgttctatgc gttgctgcgt caaggtctgc
     6541 ctgccgaggt cccgcagctt ctcgcacagg gccgacctgt acacgagggg ggcctcgccg
     6601 ccgcgatcgc gggcaatgtc atcgccgatc ccggcgagcc tacgttgaat gccgtcctcg
     6661 atgaattaca ggcgcaagcc gccgagtcgg ccgtctgggc gcaccccacc gacccggacg
     6721 ccaaatcgaa gtttcggcgc ctcctcgata ccgccgcggg gccagaagcc tcatacaccg
     6781 tgcaactcgc cttcatggag aagcacctcg cccacaccgg tacgctggcc gaattctggg
     6841 acgccgtgga cgccgacatc gaccttggcc caacagtccg ggccacctac gaatggaccc
     6901 tgcagatcaa caccttggcg aacgagcaca tgccgctgat cacggcgtta caagccaagc
     6961 gggcagacat cggcgacccg atcaagtcat ttcgtgacct ttccgcgcta gacgtagccg
     7021 actgggtcga tctcctgagt tcggggatcg gcgctcccgc atcgatcccg ctggactggc
     7081 cggaggaaga ccgcatcctg cgttacgccg agacgatcga gcgcatcgtc accgacacct
     7141 accctacaac cgtcatccac aggcgaatcg ttcgtgacca tactgctagc cctggcagca
     7201 ttgccggtgc accagacttg atccagttct tcgacgacaa ccccgccttc gagctcagcc
     7261 cgcacgagct cgacgcgtat ctcgccgacc atcccaccgc cctcgataac gtcacggacg
     7321 cagcggcgac ccgggcgaac ttgaagaagc tccagcgcct cgctcggctg gccccacgtg
     7381 ggttccagta tagagtcctc aagccgctgt accaagcagg cctccattcc gccctgcaga
     7441 tcggtcggat gacggagaaa agcttcgtgc gcaagtatgg caccaacttg gggggcgacg
     7501 acattgcgag gtcggtctac cagcgcgctc gcatgtccgc tggcatcgct ctgatggtag
     7561 cgacacagtt cgcacgcggg ttcaacaaga cgccgatctc tgtggttccc agtagtacgc
     7621 ttccagacta tgagggtcat cccgacctcg aagccttgtt cggtaacctc gacttctgcg
     7681 cctgcgacca ttgccgctcg gtatacagcg ccacggccta cttcgtcgat ctactgcaat
     7741 tcctcaagga ccagccagga aaagacgcag gtagcaacgc gctcgacgcc ctcatggagc
     7801 gccgccccga gctgaccgcg atcgagctca gctgcatcaa caccaataca gaggtcccct
     7861 acatcgacct cgtcaatgaa ctgctcgagt accacgtctc gcggcacgcc aacgcgcccc
     7921 tggcagtgtc atcgagcttc tggcagacca cctggactgc cgaggagctg gcggttcgcc
     7981 cggagcacca actctcccag gcttacaacg tcgcgctcga tacgccatat ccgtttgggc
     8041 tgcccttcga cctcgccttc gccgagacct cgctctatca tcagagcttt gacgtctccc
     8101 gcgagcgcat ggagaacgcg tatccgcata acgatccgga ttatgcggac cgccgcgccc
     8161 gcgcccgcct cggcctcgac ctgatgagct ggtatgtcat cattaactca ggcctaatca
     8221 gctggtcccc ccgggcgttc tggggcgggg agaccgcgga tgagccacag ccgcccgact
     8281 ggtggacccc gctgcgcacc gtctccacgt tcctgaagcg cgcggagatc gacatctccg
     8341 agctggccgc gctgctgcgc acgcgcttca ttggacgcgc cggcctcagc atcagctatg
     8401 cagcgccgcc cgacgattgc aacctggacg aggccgagat cctgacgctc ggtgaggccg
     8461 acctcggaaa gattcaccgc ttcatccggc tgcagcgcaa gatcggctgg acagccgatg
     8521 aactcgacct cgcactccgc actctgagcg gcgacccccc agccctcgac ggtgcgtcgg
     8581 ttcg
//
LOCUS       DASWNJ010001679         1870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_562253, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001679 DASWNJ010000000
VERSION     DASWNJ010001679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562253"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1663)
                     /locus_tag="VGB85_27890"
     CDS             complement(<1..1663)
                     /locus_tag="VGB85_27890"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY0137951.1"
                     /translation="MSRLEWLTAWRHLRALRPPPWLGRVSLMAAYLVVVGLGFLIWSA
                     SAPRPGLFGPAMFEAPPVDHAGFFGVFTLAIGGVVLAFCGLARLFNLLATIITFSVAQ
                     GCMALVLVLGLMSGLEDDLRGRLLDHKAHIRVAQHDGAGFVADDALMTALTNAPEVAG
                     ASAVLEGEVLVRTAYGRQGATLLGVDPERHRGVTALAREVEQGSYAFMMAPERIPTLG
                     TMGFEEERAGAPEPTPVPEPASDEDEGWEDPAAAALAAPPAPPVPKDLPEEPDDGGDW
                     EDPEVEIPRLRASGALPPAVAPAMPPELPAGTPVAPDAVPESPEPPPEAAPTPPEAYA
                     VLPPVLIGRELAADLGVGLGDRLQLITPVGRITPQGMVPGMLVVRVAGVFFTGIFDFD
                     RKHVYLPLPQTQAFQRAGTQVTAIEVKLREVAEIDAGKAAVTAAVAGTGLVVEDWRDQ
                     NRDLFSAMFLEKVAMSIALVFVILVAAFGILATNLMSVLEKAPEIAILKTMGCRDEQV
                     ARIFALEGLCVGVLGMLGGIATGVGVGLALGVHGLPLQGGVFYLES"
     gene            complement(1660..>1870)
                     /locus_tag="VGB85_27895"
     CDS             complement(1660..>1870)
                     /locus_tag="VGB85_27895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137952.1"
                     /translation="IALCLVLDRFGLPLGGTLQGFEELPVAVDPVEVVLVGLSSLAIV
                     WLSSLYPARIASRMRPVDALRQAER"
BASE COUNT          294 a          641 c          710 g          225 t
ORIGIN      
        1 ggctctccag gtagaacacg cccccttgca gcgggaggcc gtggaccccc agcgccaggc
       61 cgacgccgac gccggtggcg atgcccccga gcatgcccag gaccccgacg cacaacccct
      121 cgagggcgaa gatccgggcg acctgctcgt cgcggcaacc catggtcttg aggatggcga
      181 tctccggggc cttctcgagg acggacatca gattcgtggc gaggatcccg aaggccgcga
      241 cgaggatcac gaacaccagc gcgatcgaca tggcgacctt ttccaggaac atcgccgaga
      301 acaggtcccg gttctggtcg cgccagtcct cgaccaccag gccggtgccg gcgacggccg
      361 cggtgacggc cgccttgccg gcgtcgatct ctgccacttc gcgcagcttg acctcgatcg
      421 cggtcacctg ggtgccggcg cgctggaacg cctgggtctg gggcagcggg aggtagacgt
      481 gcttgcggtc gaagtcgaag atgccggtga agaacacgcc ggcgacccgt acgaccagca
      541 tgccggggac catgccctgc ggcgtgatac ggccgacggg ggtgatcagc tgcaggcgat
      601 cgccgaggcc gacgccgaga tcggccgcga gctcgcggcc gatcaatacc ggagggagca
      661 cggcgtacgc ctcggggggt gtcggggccg cctcgggggg cggctcgggt gactcgggga
      721 cggcgtccgg ggcgaccggg gttccggcgg gcagctcggg cggcatcgcg ggggccaccg
      781 cgggaggcag cgcaccgctg gcgcgcaggc gcgggatctc gacctcgggg tcctcccagt
      841 cgccgccgtc gtctggctct tcgggcaggt ccttcgggac aggtggcgcg ggcggagctg
      901 cgagggccgc tgcggcgggg tcctcccagc cctcgtcctc gtcgctcgcg ggttcgggga
      961 cgggcgtggg ctccggggcc cctgcccttt cttcttcaaa acccatcgtg ccgagcgtcg
     1021 ggatgcgctc gggggccatc atgaaggcgt agctgccctg ctcgacctcg cgggcgagcg
     1081 cggtgacgcc acggtggcgc tcggggtcga caccgagcaa ggtggcgccc tggcggccgt
     1141 aggcggtgcg caccaggacc tcgccctcga gcaccgcgct ggcgccggcg acctccggtg
     1201 cgttcgtcaa tgctgtcatc agcgcatcgt cggcgacgaa gccggcgccg tcgtgctggg
     1261 cgacccggat gtgggccttg tgatcgagga gacgaccgcg caggtcgtcc tcgaggccgc
     1321 tcatcaggcc gagcaccagc accagcgcca tgcagccctg cgcgaccgag aaggtgatga
     1381 tcgtggcgag caggttgaac agccgcgcga gcccgcagaa cgccaggacg acgccgccga
     1441 tcgccagcgt gaacacgccg aagaagccgg cgtggtcgac cggcggggcc tcgaacatcg
     1501 cggggccgaa caggccgggt cgcggggccg aggccgacca gatcagaaaa ccgaggccga
     1561 cgacgacgag gtacgcggcc atcagggaga cgcggccgag ccacggcggc ggacgcaggg
     1621 cccgcaggtg ccgccaggcg gtcagccact cgaggcggct catcgctcgg cctgccggag
     1681 cgcgtcgacc gggcgcatgc gcgaggcgat ccgcgcggga tacaggctcg acagccagac
     1741 gatcgccagc gacgagagcc cgacgagcac gacctcgacc gggtcgacgg cgaccggcag
     1801 ctcctcgaag ccctggagag tacccccgag cgggaggccg aagcggtcga gcacgaggca
     1861 cagggcgatg
//
LOCUS       DASWNJ010001680         3107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_101296, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001680 DASWNJ010000000
VERSION     DASWNJ010001680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101296"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..583
                     /locus_tag="VGB85_27900"
     CDS             146..583
                     /locus_tag="VGB85_27900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137953.1"
                     /translation="MARGDKDAYTDKQKRKAEHIAEGYSERGVSKREAKARAWATVNK
                     ESGGGNKSGSGRGTPDTKVSSRKGGRKGGASQSASNRSEAAKKGWETRRKQAGGGKKA
                     SKSAGGKKTSKKTGAKKASKKTSSKKTTSKKTGAKKTAKRRSK"
     gene            676..1698
                     /gene="hemE"
                     /locus_tag="VGB85_27905"
     CDS             676..1698
                     /gene="hemE"
                     /locus_tag="VGB85_27905"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEY0137954.1"
                     /translation="MPLQNETFLQACRGERPAHTPIWLMRQAGRYMPEYRAVRSRVSF
                     NELCRNPDLCAEVTLQPIDGLGVDAAILFSDILVVFDALGIEVRFEPSPVISTPVRTE
                     AEALALRWGDPHQHLGYVFDAVRACKRALADRVPLIGFCGAPFTTLSYLVEGQTSREF
                     EKIKGMIFSAPKVFAGLMENMTSLLCEYLRGQVLAGVDALQVFDSWAGALSPTDYREH
                     VLPHLARLVQFTRGLGVPLILFVRGNANLLAQAAELPAEVIGIDWSIDMDQAIAVTGP
                     NRVVQGNLDPMLLLGPAALVERKARACVAAGRAAKAHIFNLGHGISRHTDPAIARHLV
                     EVVHSA"
     gene            1695..2294
                     /locus_tag="VGB85_27910"
     CDS             1695..2294
                     /locus_tag="VGB85_27910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137955.1"
                     /translation="MTPPARPARSARSARSARSCALALLACLGCQTSPNRPRTSDPEP
                     PAPLPDPVHAPIDRDTGEVLEATVPLVDGDGLPLARLRGRVVVLELTATWADRWAEAY
                     PFYTRLLQQHGPDQLAVVVVAMDSERATLSPEPGVRGRGFDLGWDPQGALAARLQAAA
                     LPTVVILDRQGRIAFVQAGAPTGATRAIEDGVAQALASR"
     gene            2341..>3107
                     /locus_tag="VGB85_27915"
     CDS             2341..>3107
                     /locus_tag="VGB85_27915"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY0137956.1"
                     /translation="MLERLNAVTTDPKLQAAIVLLASFVVAFVTEVVFRGVLLIFVQN
                     TKTHLDDIIVANVRRPLFLSVLFLGLSLSASLLHPEAWVATALNAALISLAILLWAGA
                     AIRICAAVLRHMGESPGASGVLQPRTLPLFDMMAKILLAGAAVYAIMVAWGLDLTGWL
                     ASAGIVGIAVGFAAKDSLANLFAGIFIIADAPYKLGDFIMFEDGLRGRVTEIGLRATR
                     ILTNDDIEINIPNQLIGNSRVVNESAGRHEKERIRLPV"
BASE COUNT          490 a         1230 c          955 g          432 t
ORIGIN      
        1 gcaatagacc cgcgtcgcgc tcgactgcac gaccggcgaa ttgcccgcca cgtctccacc
       61 ggcgatcccg gcgcgcttgt gatcccccgc gcctcgcttt gacacatccg tgccttcgcc
      121 gcatgaccac gcgaaggagc aacccatggc acgcggagac aaggacgctt acaccgacaa
      181 gcagaagcgc aaggcagagc acatcgccga gggctacagc gaacgcggcg tctccaagcg
      241 ggaggccaag gcccgcgcct gggcgaccgt caacaaggag tccggcggcg gcaacaagtc
      301 cggcagcggc cgcggcaccc ccgacaccaa ggtgtcctcg cgcaagggcg gccgcaaggg
      361 cggcgccagc cagagcgcca gcaaccgcag cgaggccgcc aagaagggct gggagacccg
      421 ccgcaaacaa gccggcggcg gcaagaaggc cagcaagtcc gctggcggca aaaaaaccag
      481 caagaagacc ggcgccaaga aggccagcaa gaagacctcc agcaagaaga ccaccagcaa
      541 gaagaccggc gccaagaaga ccgcgaagcg ccgaagcaag taggccacga agcgccagag
      601 caagtaggcg agtcgcgccg gcgagcgcgc gccaggctat ccgcagcgcc gggccgcggg
      661 tatagtcccg cgccgatgcc tctgcaaaac gagacctttt tgcaagcgtg ccgcggcgag
      721 cgaccggcac acacaccgat ctggctcatg cgccaggccg gtcgttacat gccggagtac
      781 cgcgcggtgc gcagccgggt ctcgttcaac gagctttgtc gcaacccgga cctgtgcgcc
      841 gaggtcacct tgcagccgat cgacggcctc ggcgtcgacg cggcgatcct gttctcggac
      901 atcctcgtcg tgttcgacgc cctcggcatc gaggtccgct tcgagccctc gcccgtgatc
      961 tcgacccccg tgcgaacgga ggccgaggcg ctcgcgctgc gctggggcga cccgcaccag
     1021 cacctcggct acgtgttcga cgccgtgcgc gcgtgcaagc gagcgctcgc cgaccgcgtc
     1081 ccgctgatcg gcttctgcgg cgcccccttc accaccctca gctacctcgt cgagggccag
     1141 accagccgcg agttcgagaa gatcaaaggc atgatcttct ccgccccgaa ggtgttcgcc
     1201 gggctgatgg agaacatgac cagcctgctg tgcgagtacc tgcgcggcca ggtgctcgcc
     1261 ggcgtcgacg ccctgcaggt gttcgacagc tgggccggcg ccctctcgcc caccgactac
     1321 cgcgagcacg tgctgcccca cctcgcccgc ctcgtccagt tcacccgcgg cctcggcgtc
     1381 ccgctgatcc tgttcgtccg cggcaacgcc aacctgctcg cgcaggccgc cgagctgccc
     1441 gcggaggtca tcggcatcga ctggtcgatc gacatggacc aggcgatcgc cgtcaccggc
     1501 ccgaaccgcg tcgtgcaggg caacctcgac cccatgctgc tgctcggccc cgccgcgctc
     1561 gtcgaacgca aggcccgcgc ctgcgtcgcg gccggccgcg ccgcgaaggc ccacatcttc
     1621 aacctcggcc acggcatctc ccgccacacc gaccccgcga tcgcccgcca cctcgtcgag
     1681 gtcgtccact ccgcatgacc ccgcccgctc gtccagctcg ttcagctcgt tcagctcgtt
     1741 cagctcgttc gtgcgcgctc gccctgctgg cctgcctcgg ctgccagacc agccccaacc
     1801 gcccgcgcac cagcgacccc gagccgcccg ccccgctgcc cgaccccgtc cacgcaccga
     1861 tcgaccgcga caccggcgag gtcctcgagg ccaccgtccc gctcgtcgac ggcgacggcc
     1921 tcccgctcgc ccgcctgcgc ggccgcgtcg tcgtgctcga gctgaccgcc acctgggccg
     1981 accgctgggc cgaggcctac ccgttctaca cccgcctgtt gcagcagcac ggacccgacc
     2041 agctcgccgt cgtcgtcgtc gccatggaca gcgagcgcgc gaccctgagc cccgagcccg
     2101 gcgtgcgcgg ccgcggcttc gacctcggct gggacccgca aggcgccctg gccgcccgcc
     2161 tgcaggcagc tgccctgccg accgtcgtca tcctcgaccg ccagggccgg atcgccttcg
     2221 tgcaggccgg cgcgccgacg ggagccaccc gtgcgatcga ggacggcgtc gcccaggccc
     2281 tcgcctcgcg ctgatcttgt tcaggccagc caccgcgggc atcctgtgta actcgcgcgc
     2341 atgctcgaac gcctcaacgc ggtcaccacg gacccgaagc tgcaggcggc gatcgtcctg
     2401 ctggcgtcgt tcgtcgtcgc cttcgtcacc gaggtcgtgt tccgcggcgt cctgctgatc
     2461 ttcgtccaga acaccaagac ccacctcgac gacatcatcg tcgccaacgt gcgccgcccg
     2521 ctgttcctca gcgtgctgtt cctcggcctc tcgctctcgg cctcgctgtt gcatccggag
     2581 gcgtgggtgg cgaccgccct caacgccgcg ctgatcagcc tggcgatctt gctgtgggcc
     2641 ggcgctgcga tccggatctg cgccgccgtg ctgcgccaca tgggcgagag ccccggcgcc
     2701 tccggcgtgc ttcagccgcg caccttgccg ctgttcgaca tgatggcgaa gatcctcctc
     2761 gccggcgccg ccgtctacgc gatcatggtc gcctggggcc tcgacctcac tggctggctc
     2821 gcctccgccg gcatcgtcgg catcgcggtc ggcttcgccg ccaaggactc gctcgccaac
     2881 ctgttcgccg gcattttcat catcgccgac gccccctaca agctcggcga cttcatcatg
     2941 ttcgaggacg gcctgcgcgg ccgcgtcacg gagatcggcc tgcgcgcgac ccggatcctc
     3001 accaacgacg acatcgagat caacatcccc aaccagctga tcggcaactc ccgcgtcgtc
     3061 aacgagtccg ccggccgcca cgagaaggag cgcatccggc tgcccgt
//
LOCUS       DASWNJ010001681         2277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_134865, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001681 DASWNJ010000000
VERSION     DASWNJ010001681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134865"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="VGB85_27920"
     CDS             <1..1035
                     /locus_tag="VGB85_27920"
                     /inference="COORDINATES: protein motif:HMM:NF036933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HEY0137957.1"
                     /translation="SLLLPTSVWDTSYIAVNAGPAGESGTPLLTFIAREDDTHVTLRP
                     NASVKPGIGVKGGPAGVPLTYTMDAGESIQLNQVHELTGSPLFADKPFAVFGGSTCLN
                     IPIDAYACDGAHQQIPPVRALGSAYAAVRYRDRVVGKVESPPWRLVGAAKDTTLVWTP
                     AKPPGAPSTLTLGQVADFNATGPFHVRSQDDDHPFYASAYMTGGQTFGGAGDPDWVNL
                     VPPAQFQKRYIFFTDPTYSETELVVVRREDDGGFADVILDCTGPLVAWKPLAAGIEWT
                     RVQLVTGDFNKVGKCANGRHEIHSDKPFGVTVWGWGSAASELIYTKFVSYAYPAGATI
                     RSINTVQITE"
     gene            1073..>2277
                     /locus_tag="VGB85_27925"
     CDS             1073..>2277
                     /locus_tag="VGB85_27925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137958.1"
                     /translation="MHGPRQLWDAFFASDPELARLRQAARVTLTAMLAALVLDVIAVT
                     IGESTSVALIGVNVAMLGSAIVNDPLPREQMLTYLLAPVTASLAIAAGTLAAPHDLGR
                     TALLLALVFVAVLVRRFGPRGLALGTISLLACFFSLFFQARPDQLPLMIAGVFVASAL
                     GFVVRFGLMRDPPEALLRRRLTGLRRTISALLGELESAAAEGRADPHVGAVQRRLGQL
                     NEAALAIEELPNLGLPGQAPGRVLALEVAVEGLVVALRHWLVAPQALHLRAVARMLAA
                     AQALVDAPDPAALARALDELQAKPTPELAWLYDELVALLIALRDLTAPVAGEPPPPAT
                     PPPPPAPVPTLRPAIQATVAAALALLLGGLVSSERAFWAVLAAFMVFTRANTLGAMLA
                     RAWQRTIGTL"
BASE COUNT          287 a          941 c          720 g          329 t
ORIGIN      
        1 tcgctgctgc tgccgaccag cgtgtgggac accagctaca tcgccgtcaa cgccggcccc
       61 gcgggtgagt ccggcacccc gctgctgacc ttcatcgccc gcgaggacga cacccacgtc
      121 acgctgcgcc ccaacgcgag cgtcaagccc ggcatcggcg tcaagggtgg tccggccggc
      181 gtaccgctga cctacacgat ggacgccggc gagagcatcc agctcaacca ggtccacgag
      241 ctgaccggct cgccgctgtt cgccgacaag cccttcgccg tgttcggcgg gtcgacctgc
      301 ctcaacatcc cgatcgacgc ctacgcctgc gacggcgctc accagcagat cccgccggtg
      361 cgcgcgctcg gcagcgccta cgccgctgtc cgctaccgcg accgcgtcgt cggcaaggtc
      421 gagtcgccgc cgtggcgcct cgtcggcgcc gccaaggaca ccaccctcgt gtggaccccg
      481 gccaagcccc ccggcgcacc gagcacgctc accctcggac aggtcgccga cttcaacgcg
      541 accggcccgt tccacgtccg cagccaggac gacgaccacc cgttctacgc ctccgcctac
      601 atgaccggcg gccagacctt tggcggcgcc ggcgaccccg actgggtcaa cctcgtcccg
      661 cccgcgcagt tccagaagcg ctacatcttc ttcaccgacc cgacctactc ggagaccgag
      721 ctggtggtcg tgcgccgcga ggatgacggc ggcttcgccg acgtgatcct cgactgcacg
      781 ggcccgcttg tcgcctggaa gccgctcgcc gccggcatcg aatggacgcg cgtgcagctc
      841 gtcaccggcg atttcaataa agtcggcaag tgcgccaacg gccgccacga gatccacagc
      901 gacaagccgt tcggcgtcac cgtgtggggc tggggctcgg ccgcgagcga gctgatctac
      961 accaagttcg tgtcctacgc ctatccggcc ggcgcgacga tccgatcgat caacaccgtc
     1021 cagatcaccg agtgacgcgg cgtcggccgc gtgtacgtgc atacttcgcg gcgtgcacgg
     1081 gccccggcag ctgtgggacg ccttcttcgc ctccgacccg gagctcgcgc ggctgcgcca
     1141 ggccgcccgc gtcaccctga ccgcgatgct cgccgccctc gtgctcgacg tcatcgccgt
     1201 cacgatcggc gagtcgacca gcgtcgcgct gatcggcgtc aacgtcgcga tgctcggctc
     1261 cgcgatcgtc aacgacccgc tgccgcgcga gcagatgctg acgtacctgc tcgcgccggt
     1321 caccgccagc ctggcgatcg ccgccggcac cctcgccgcg ccgcacgacc tcgggcgcac
     1381 cgccctgctg ctcgcgctgg tgttcgtcgc cgtgctcgtg cgccgcttcg gcccgcgggg
     1441 cctcgcgctc ggcacgatct cgctgctcgc ctgcttcttc tcgctgttct tccaggcccg
     1501 ccccgaccag ctcccgctga tgatcgccgg cgtcttcgtc gcctccgcgc tcggcttcgt
     1561 ggtccgcttc ggcctcatgc gcgacccacc cgaggcgctg ctgcgccggc gcttgaccgg
     1621 cctgcgacgc acgatctccg cgctgctcgg cgagctcgag tcggccgccg ccgagggccg
     1681 cgccgacccc cacgtcggcg ccgtgcagcg ccggctcggc cagctcaacg aggccgcgct
     1741 ggcgatcgaa gagctgccca acctcggcct gcccggccag gcccccggcc gcgtgctcgc
     1801 gctcgaggtc gccgtcgagg gcctcgtcgt cgcgctgcgc cactggctcg tcgcgccgca
     1861 agcactgcac ctgcgggccg tcgcgcgcat gctggcggcc gcccaggcgc tcgtcgacgc
     1921 ccccgacccc gccgcgctgg cgcgtgcgct cgacgagctg caagcgaagc ccacccccga
     1981 gctcgcgtgg ctgtacgacg agctcgtcgc gctgttgatc gccctgcgag acctgaccgc
     2041 cccggtcgcc ggcgagccgc cgccacccgc gacgccgcca ccgcccccgg ccccggtccc
     2101 gaccctgcgc cccgcgatcc aggcgaccgt cgcggccgcg ctcgcgctgc tgctcggcgg
     2161 cctggtctcg agcgagcgcg ccttctgggc cgtgctcgcc gccttcatgg tgttcacgcg
     2221 ggccaacacc ctcggagcca tgctcgcgcg cgcctggcag cgaacgatcg ggaccct
//
LOCUS       DASWNJ010001682         2844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_523687, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001682 DASWNJ010000000
VERSION     DASWNJ010001682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523687"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VGB85_27930"
     CDS             complement(<1..690)
                     /locus_tag="VGB85_27930"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY0137959.1"
                     /translation="MGPELMQHLADPDVWISLLTLSVLEIVLGLDNIIFISILVGKLP
                     NESRDRARRIGLMGAFVMRIGLLSVLAWVMHLKEPLFTVLGNEITGKELVLLLGGLFL
                     LYKATKEIHERVDGPLTEQEMAAQKASSVAAVVFQILMLDLVFSIDSVVTAVGMAQHL
                     WVMIAANVIALGVMLAASKSIAAFVDEHPTVKILALSFLLMIGLLLVAEAFEIHVPKG
                     YVYFAMAYSVVV"
     gene            1066..1674
                     /locus_tag="VGB85_27935"
     CDS             1066..1674
                     /locus_tag="VGB85_27935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137960.1"
                     /translation="MDCAEVASPQPVDLLRDEAEPGAPGLSTLTLQIEAFEQKDGASH
                     YCLAALAGERRVGFSLELRCSDRDPLPIPEHDVELSRCEVILKSTGEESDRFVAAVAA
                     VYDLDVGRMRGGRRGHMPDELEFHALCLAGEPGRPQLGPLQLLLLYSGSEPARRSDVM
                     KGDFEWFLRIDLTQGEAAFVDRAPEHHASIVAALSGGLSWRH"
     gene            1774..2400
                     /locus_tag="VGB85_27940"
     CDS             1774..2400
                     /locus_tag="VGB85_27940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137961.1"
                     /translation="MDPKQHLRARIARNLQRVRGLIAASTRLAAMDGPVSEEPADILR
                     AAVVLLHATMEDVLRTLEEVALEPASPATLKAFGIEYGRKKPEKLALWELAEKYGGAS
                     ISDVVRLATVVYLDGQTYNNGDELAGVLGRLKIVPKPLLDRFAGDLEGMMRRRHRIVH
                     RTDLSGSRVGDSLSLLTETLHPDEVLSWIETVDQFVHAVLERYARSAA"
     gene            2397..2666
                     /locus_tag="VGB85_27945"
     CDS             2397..2666
                     /locus_tag="VGB85_27945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137962.1"
                     /translation="MMTRDALRASLERMNLSDAEIRVTGDPGRIIGIIASPDFEGLNG
                     AVRQGKLWRHLLDQLSPDQLEQIEFVFTYTPRELAALDRGERPSA"
BASE COUNT          462 a          942 c          998 g          442 t
ORIGIN      
        1 gacgacgacg ctgtaggcca tcgcgaagta cacgtagccc ttgggcacgt ggatctcgaa
       61 ggcctcggcg acgagcagca ggccgatcat cagcaggaag ctgagcgcga ggatcttgac
      121 ggtcgggtgc tcgtcgacga acgcggcgat cgacttcgag gccgcgagca tgacgccgag
      181 cgcgatgacg ttggcggcga tcatgaccca caggtgctgg gccatgccga ccgcggtgac
      241 gaccgagtcg atcgagaaca cgaggtcgag catcaggatc tggaacacca cggcggcgac
      301 gctcgaggcc ttctgggcgg ccatctcctg ctcggtcagg ggcccgtcga cgcgctcgtg
      361 gatctccttg gtcgccttgt agagcaggaa caggccgccg agcagcagca ccagctcctt
      421 gcccgtgatc tcgttgccga gcaccgtaaa cagcggctcc ttgaggtgca tgacccaggc
      481 gagcacgctc agcaggccga tgcgcatcac gaaggcgccc atcaggccga tccgtcgcgc
      541 acgatcgcgg ctctcgttgg gcagcttgcc gacgaggatc gagatgaaga tgatgttgtc
      601 caggcccagc acgatctcca gcaccgacag cgtcagcagg ctgatccaca cgtccgggtc
      661 ggcgaggtgc tgcatcaatt cggggcccat gcgcgcaggg tatcgcgttt gccggctcca
      721 ggccccgcac cggccgatgc cgcgacaacg cgcgcgaggt ggctcgcccg acgccccccg
      781 gctcatcccg tagcgcaacc cccgcgtcgg cccacggggc gcgacgcccc gcgacttgcg
      841 acacagcggt cgtatgcgcc gcgcagcagg ccgccacgcg ccccgcggcg aaccagcggg
      901 cccccgcggc ggcgcctccg ggagcgtgca agtgcggtgc gtcgcgggcc gaccgcgggg
      961 cgcgtgctag gttcgcggtg tgccccgggc gagtcgggca cagcgaacgc ggacgcatcg
     1021 cgtgttccgc ttcgacctgc gtatcgtatg aaaggagcgc tcgccatgga ctgcgccgaa
     1081 gttgcgtctc ctcaacctgt cgacctcctg cgcgacgaag ccgagccggg agctcccggc
     1141 ctatcgaccc tgaccttgca gatcgaagcc ttcgagcaga aggacggtgc cagccactat
     1201 tgtctggcgg ctctggccgg cgagcgccgg gtcggcttct cgctcgagct gcgctgctcc
     1261 gaccgcgacc cgctgccgat cccggagcac gacgtcgagc tctcgcgctg cgaggtgatc
     1321 ctcaagagca cgggcgagga gagcgaccgc ttcgtcgccg cggtcgcggc cgtctacgac
     1381 ctcgatgtcg gccgcatgcg gggcggtcgc agggggcaca tgccggacga gctcgagttc
     1441 cacgcgctgt gcctcgcggg cgagcccggg cggccgcagc tcgggcccct gcagctgctg
     1501 ttgctgtaca gcgggagcga gccggcgcgc cgcagcgacg tcatgaaggg tgacttcgag
     1561 tggttcctgc gcatcgacct gacgcagggc gaggcggcct tcgtcgaccg cgcgccagag
     1621 caccacgcgt cgatcgtcgc ggcgctctcg ggcggcctct cgtggcggca ctgagcgggt
     1681 gagggcgagc tgcaacaata cggcggcgtg gtatgagcct cgcccgtgaa gtcgccttga
     1741 cctgcccggg cgcgctgcta gctttcgcgc ctcatggatc cgaagcagca cctcagggca
     1801 cggattgccc gcaacttgca gcgcgtacgc gggctgatcg ccgcatccac gcggctggcg
     1861 gcgatggatg ggccagtctc ggaggagccc gccgacattc tgcgagccgc cgtcgtgctg
     1921 cttcatgcga cgatggagga cgtgctgcgc accctcgagg aggtcgccct cgagccggcc
     1981 tcgccggcga cgctcaaggc gtttgggatc gagtacgggc gaaagaagcc cgagaagctc
     2041 gcgttatggg agctcgccga gaagtatggc ggagcgagca tcagcgacgt ggttcgcctg
     2101 gcgacggtcg tgtacctcga tggccagact tacaacaacg gtgacgagct tgcgggtgta
     2161 ctgggacgcc tgaagatcgt tccgaagccg ctgctcgatc ggttcgcagg tgacctcgag
     2221 gggatgatgc gtcgccgtca ccggatcgtt caccggacgg acctctcggg ctctcgggtg
     2281 ggggattcct tgagtctcct caccgaaaca ctgcatccgg atgaggtatt gtcgtggatc
     2341 gagaccgtgg accagttcgt gcacgcggtt ttggagcgct atgcgaggag tgcggcatga
     2401 tgaccagaga tgctttgcga gccagcctcg agcggatgaa cctctccgac gcggagatcc
     2461 gcgtgaccgg cgaccccggg aggatcatcg gcatcattgc ctctccggac ttcgaggggc
     2521 taaacggggc cgttcgtcag ggcaagctct ggcgccacct actcgaccag ctatcacctg
     2581 accagctcga gcagatcgag ttcgtcttca cgtacacgcc gcgcgagctg gcggcgctgg
     2641 accgcggcga gcgcccgtcc gcgtgagcct ttagcgcccg cgcgcgagcg cggcgacgag
     2701 gttgcggcgc tcgctcgaca gcgccgggcg acgcagatgt ttgaccatgt cgtcgaggcc
     2761 ggagagcgtc agctggaaca tcgggaacac ccgcgcgatc tcggcgatcg tgccggagcg
     2821 ccacagaccg ggcggctccg ggtt
//
LOCUS       DASWNJ010001683         1642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_452098, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001683 DASWNJ010000000
VERSION     DASWNJ010001683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452098"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VGB85_27950"
     CDS             <1..183
                     /locus_tag="VGB85_27950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0137963.1"
                     /translation="LERAIQVHGALGLTDDTVLAYFYTRERGARIYDGPDEVHKMVVA
                     RRILGRYGKQAKGGAR"
     gene            180..1244
                     /locus_tag="VGB85_27955"
     CDS             180..1244
                     /locus_tag="VGB85_27955"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEY0137964.1"
                     /translation="MSAAQEHWIDAAKPVREALDLERLTPYLQNALGLSGDIQVQQFP
                     GGHSNLTYLLRVGERELVLRRPPFGAQIKTAHDMDREFTILGKLAPVYPRVPRPLLLC
                     DDESVLGARFYLMERVRGVVLRARLPAGLEIDAALARRLSQAAADTLAELHALDIDAT
                     GLRALGHPEGYVRRQVEGWTKRYAAAVTDEIPAMDRVAAWLAANMPESGPPTLLHNDF
                     KYDNLVLDPDDLTRVRGVLDWEMATVGDPLMDLGTALCYWVEADDPPAVRAFAFGPTA
                     VPGSYTRREFAEHYARVTGRSLERLDFYRAFGLYKTAVVAQQIYLRFVRGHSKDPRFA
                     LMIEGVRALAGQAERAIAGE"
     gene            1289..>1642
                     /locus_tag="VGB85_27960"
     CDS             1289..>1642
                     /locus_tag="VGB85_27960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137965.1"
                     /translation="MRSARLLSLSVLVVLSACSDVDTASSTSAAETGTTGENPDTGEA
                     TSTTSTTAPATTTDPTTGASTGPTSTATTGDPGADDSSGATGSTDGETADAQELPPIE
                     SVEILEAWLAAGSYRT"
BASE COUNT          248 a          621 c          560 g          213 t
ORIGIN      
        1 ctcgagcgcg cgatccaggt ccacggcgcg ctcggcctga ccgacgacac cgtgctcgcg
       61 tacttctaca cccgcgagcg cggcgcgagg atctacgacg gccccgacga ggtgcacaag
      121 atggtcgtcg cgcgccggat cctcgggcgc tacggcaagc aggcgaaggg gggtgcccga
      181 tgagcgccgc gcaggagcac tggatcgacg ccgcgaagcc cgtgcgcgag gccctcgatc
      241 tcgagcgcct cacgccatat ttgcaaaatg ccctggggct gtccggggac atccaggtcc
      301 agcaattccc gggcggccac tccaacctca cctacctgct cagggtcggc gagcgcgagc
      361 tggtgctgcg ccggccgccg ttcggcgcgc agatcaagac ggcccacgac atggaccgcg
      421 agtttacgat cctcggcaag ctcgcgccgg tgtacccgcg ggtcccgcgg ccgctgctgc
      481 tgtgcgacga cgagtctgtc ctcggggcca ggttctatct gatggagcgc gtgcgcggcg
      541 tggtgctgcg cgctcgcttg cccgccggcc tcgagatcga cgccgcgctc gcgcgccggc
      601 tgtcgcaggc ggccgccgac accctcgccg agctgcacgc gctcgacatc gacgcgaccg
      661 gcctgcgcgc gctcgggcac cccgagggct acgtgcgccg ccaggtcgag ggttggacga
      721 agcgctacgc cgccgcggtc accgacgaga tccccgcgat ggaccgcgtc gccgcgtggc
      781 tggccgccaa catgccggag tccggcccgc cgacgctgct gcacaacgac ttcaagtacg
      841 acaacctcgt gctcgacccc gacgacctca cgcgcgtgcg cggcgtgctc gactgggaga
      901 tggcgaccgt cggcgacccg ctgatggacc tcggcaccgc gctctgttac tgggtcgagg
      961 ccgacgaccc gccggccgtg cgggccttcg cgttcgggcc gaccgcggtg ccgggcagct
     1021 acacgcgccg cgaattcgcc gagcactacg cgcgggtcac cggccgcagc ctcgagcgcc
     1081 tcgattttta ccgcgcgttc ggcctctaca agaccgccgt cgtcgcccag cagatctatc
     1141 tccgcttcgt ccgtgggcac agcaaggacc cgcgcttcgc cctgatgatc gagggcgtgc
     1201 gtgcgctcgc cggccaggcc gagcgcgcca tcgcgggcga gtgaacgagc cgcgtaacat
     1261 gcggtctccc gtccacgttg gcgtccgcat gcgatcggcc cgcctcctgt ccctctccgt
     1321 cctcgtcgtg ctcagcgcct gcagcgacgt cgacacggcg agctcgacga gcgccgcgga
     1381 gacgggcacg acgggtgaaa accccgacac cggcgaagcg acctcgacca cgagcaccac
     1441 ggctccggcc acgacgactg acccgacgac cggcgcgagc accggaccga cgagcaccgc
     1501 cacgaccggc gatcccggcg ccgacgacag cagcggtgcc accgggagca cggacggcga
     1561 gacggcggat gcgcaggagc tgccgccgat cgagagcgtc gagatcctcg aggcctggct
     1621 cgccgccggc agctaccgga cc
//
LOCUS       DASWNJ010001684         6270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_595686, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001684 DASWNJ010000000
VERSION     DASWNJ010001684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595686"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VGB85_27965"
     CDS             complement(<1..260)
                     /locus_tag="VGB85_27965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137966.1"
                     /translation="MSIRSEGTSWPTRRRFLLQLLATAVAAQAGGCGDAQGGTEGGSS
                     GAGSSGDTPTTGDTPTTGDTSTGEPQPELPGDPFTLGVASGDP"
     gene            complement(331..624)
                     /locus_tag="VGB85_27970"
     CDS             complement(331..624)
                     /locus_tag="VGB85_27970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137967.1"
                     /translation="MATDIIVKHRVTDFKTFKPGFDALVRQFAQQTGAQGHHVHHELG
                     DPNHVIVTVLGVADPDKARSLFASQDFQAEMGKIGVCSAPDITFGSRVEDVRY"
     gene            854..4072
                     /locus_tag="VGB85_27975"
     CDS             854..4072
                     /locus_tag="VGB85_27975"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0137968.1"
                     /translation="MSSQIRPGPTRAHRLRLWVGSLVLASCGGASEVASMQVCDRVGT
                     LCVTGDEHGGLGLSGARGATGDLDGDGVRDLVNVSGSLSIAWGDGDAREYLLFPEGVA
                     DAEIGDVDGDGDLDVVFVTTHPASLRVLENQGARRFLERAAVVVPGYGASLWLGQLDG
                     DGPLDAVVASSGNDGALTVFTAGLTRVQPIAVGPGLSAVEVGDLDGDGRLDIVATDDF
                     EMAIRVALADGAGFAAATKISTGRAPAHLQLYDFDGDGRLDVLTQGDSPGIWFHPGDG
                     GGGLAAPRVVVVQESTSRGFAAHRDEQGRRWLITLDNYPLVASQLDDADRVVRRVVAG
                     GHLETYRIAMDGGSLLTQGTFYGQRYSLDPSHVFTELRHGGPGTNRSIALADLDADGE
                     PELLSVEDDVVLRRGLDDESWDEGQPLYAAQGHIYSLVVADVTGDGAPDLVLSGDAPS
                     LQVAVGVGDGTFTAGGAPVQLDGSPGRMYAGLSAPGEAAAVAVAVYDSDDPGVDVLRF
                     DAAGELIDQARVVAADGVVTLTSADLEADGDEDLVVLTRDAHEASSLSLVPRLADGWG
                     PARTRSLADLHSESAPDRPLNGAQLVTGDIDLDDRVDAMLVASGVVVHLLDIGADDPP
                     APRMREYPGLAAPDALALADVDGEGPLDLVSCSYGGLRILLQASDGEVQPQVAHDQFV
                     RSCALHVDPDDRRATAATATDRGYALLRPDFAPALARTDSFEGGMSFGGRVEAGDIDA
                     DGVADIVVADRPWGWGGSTAVLWGSAGGQARRATRQAGMPFRVAELAVAPLDDRPGDE
                     VVMAWTTGNMSIGAGGAVQIWTHAEGSLQQRFTTNPRLGSQVVGVGVQRRAGAPSDVI
                     LLDRFAPGELGVIAVPRGDDGDFIADADVLLWSGPWIDDAPAMVIADFDGDGYEDIAV
                     SYGALEGVALTWGDRERTPAVTTVPIPVAARTNLASADLDDDGAAELFVGTEQGVVRI
                     AFAGREPKPREKLLADSAVLNLTITDVDGDGITDIVAHNSWWLSINLRAPTGIYFAKL
                     LFLPLSQLRAADIDGDQILDFIGIFGGEVITRRSTGAPGPPL"
     gene            complement(4097..4960)
                     /locus_tag="VGB85_27980"
     CDS             complement(4097..4960)
                     /locus_tag="VGB85_27980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137969.1"
                     /translation="MSTRPARTPKSDELPVRVAAELVRLGVTFDDTVDTTAFGPGTLG
                     RLARARWPDLELAAPGPAGGPPIEQFRGVPLHFLENVVPRQLCIGMYTSRGKPRAMMI
                     ANGDARDPQVYPLALDEPDTWAGSIYSGYMPLSRFLRSLGPPTSGLPKLLLRQIVRLG
                     GVPPAEDAIATRQVNTVAGPRTLSRVVSGLLAVKWPADRTFVSGEPAAALSLGWSEVT
                     ERLASGYDGPLLAIAHDVHQYFAVVRVDDISDDPLVHRLDHDGSSTLDAGVPLSRFLA
                     AIKRASPGRRS"
     gene            5088..>6270
                     /locus_tag="VGB85_27985"
     CDS             5088..>6270
                     /locus_tag="VGB85_27985"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0137970.1"
                     /translation="MVTILHVSDMQFGAQHRFGRLGLGGADEAFDTLLRRLTDDLDRM
                     RDEEGIMPDLVALTGDLAEWARPKEYADVLQFCEGLRAHLQLAADRLLVIPGNHDVNR
                     DHCQSYFAYCAGNDEAPQEPWWPKWGPYTNFFGKLYGAVERYEFKELAPYTWFELPEL
                     KVVVAGLNSTMRESHEDRDPGDPASIFGHYGYLGEAQLDWFGRKLDDAAHKGWLRIGL
                     VHHNAQRNAVDDDENLRDVGMLVDRLGGRLNLLLHGHTHRGTLGWLRRDLPVISTGSA
                     GVKAGQRPEEVPNQYQIVQVGPAGFRCWARQYTPAARKWIGDTRVSQDGSDWRHAHEV
                     AFPRTSGTFGTRESRGRPGASPFASDDEEEDPHRRAREANDLVAQVMASCRVRDEAGQ
                     VQ"
BASE COUNT          882 a         2106 c         2362 g          920 t
ORIGIN      
        1 gggtcgcccg aggcgacgcc gagcgtgaag gggtcgcccg ggagctcggg ctggggctcg
       61 ccggtcgagg tgtcgccggt ggtcggcgta tcgccggtgg tcggcgtgtc gccactgctg
      121 ccggcgcccg acgaccctcc ctcggtgccg ccctgcgcgt caccgcaccc gcccgcctgg
      181 gcagcgaccg cggtcgccag cagctgcagg agaaatcgtc gtcgcgtcgg ccatgaagtt
      241 ccctcggagc ggatggacat cgcgcgcgag tataaccgct gcgcaaccgc tatcctcggg
      301 ggacgtgccg tcccggccgc gtggtgtgcc tcagtagcgt acgtcctcga cgcgtgagcc
      361 gaaggtgatg tcgggcgcgc tgcacacccc gatcttgccc atctcggcct ggaagtcctg
      421 ggacgcgaac aaggagcgag ccttgtccgg gtcggcgacg ccgagcacgg tgacgatcac
      481 gtgattgggg tcgccgagct cgtggtggac gtgatgcccc tgcgcgccgg tctgctgcgc
      541 gaactggcgg acgagggcgt cgaagccggg cttgaaggtc ttgaagtccg tcacccggtg
      601 cttgacgatg atgtctgtgg ccatgtgaac tgctctccgg ggcgccggtg tcgccagcgc
      661 cgcccgcaaa cctgcgcggc ctggcgatcc gcgaccaccc gcgaccggcc tcctcgcaag
      721 gagacagccg cagacggccg cctggacgcg agttcggcca gggccgaaga ggggcgatcc
      781 ggcgcgtcac gaaaacgcgc agatcgcggg cagggaccgg acgcaggcgc tggacgatgt
      841 atcctcggcg agagtgtcgt cgcagatccg acccggtccc acgcgcgcgc atcggctgcg
      901 gctgtgggtc gggtcgctgg tgctcgcgag ctgcgggggc gcgagcgagg tcgcgtcgat
      961 gcaggtctgc gatcgcgtcg gcacgctgtg tgtcacgggc gacgagcatg ggggcctcgg
     1021 gctctccggc gcgagggggg cgaccggcga cctcgacggc gacggcgtgc gcgacctggt
     1081 caacgtgtcg ggcagcctct cgatcgcgtg gggcgacggc gatgcgcgcg agtatctgct
     1141 gttcccggag ggggtcgcgg acgcggagat cggcgacgtc gatggtgacg gcgacctcga
     1201 cgtcgtgttc gtcaccaccc atcccgcgag cctgcgggtg ctcgagaatc agggggcacg
     1261 gcggttcctc gagcgggccg ccgtcgtggt ccccgggtat ggtgcgagcc tgtggctcgg
     1321 gcagctcgac ggtgacggac cgctcgacgc ggtggtcgcg agcagcggga acgacggcgc
     1381 gctcaccgta ttcaccgccg gcctgacgcg cgtccagccg atcgcggtcg gtccggggct
     1441 cagcgcggtc gaggtgggcg acctcgacgg tgatggccgc ctcgacatcg tcgcgacgga
     1501 cgacttcgag atggcgatcc gcgtcgcgct cgcggacggg gccggcttcg cggccgcgac
     1561 caagatctcg accggacgcg cacccgcgca tctgcagctg tatgacttcg atggcgacgg
     1621 ccggctcgac gtcctcacgc agggcgacag cccggggatc tggtttcatc cgggtgacgg
     1681 cggcggcggt ctggctgcgc cgcgcgtcgt ggtcgtgcag gagtccacga gccgcggctt
     1741 cgcggctcac cgtgacgagc agggccgacg ctggttgatc accctcgaca attatccgct
     1801 ggtcgccagt cagctcgacg acgccgaccg ggtggtccgc cgcgtggtcg cgggcgggca
     1861 cctggagacg tatcgcatcg ccatggacgg cggctccctg ctgacgcagg ggacctttta
     1921 tgggcagcgc tactcgctcg atccgtcgca tgtgttcacc gagctgcggc acggcggtcc
     1981 cgggaccaat cgctcgatcg cgctcgcgga cctcgacgcg gacggcgagc ccgagctgct
     2041 gtcggtcgag gatgacgtgg tgcttcgccg cggactcgac gacgagtcgt gggacgaagg
     2101 acagccgctg tatgcagcgc aggggcatat ttattcgctc gtcgtcgccg acgtcaccgg
     2161 cgacggtgcg cccgacctcg tgctcagcgg cgacgcgccc tcgttgcagg tggcggtcgg
     2221 ggtcggcgac ggcacgttca cggccggggg agcgcccgtg cagctcgatg gcagccccgg
     2281 gcgcatgtac gcggggctct cggcgcccgg cgaggcggcg gccgtggcgg tcgccgtgta
     2341 tgactccgac gatccgggcg tcgacgtgct gcgcttcgac gccgcgggcg agctgatcga
     2401 ccaggcgcgt gtggtcgcgg ccgacggggt cgtcacgctg acctcggccg acctcgaggc
     2461 cgacggcgac gaggacctcg tcgtcctgac ccgggacgct cacgaggcct cctcgctgag
     2521 cctcgtcccg cggctcgcgg acggctgggg tccggcgcgt acgcgctcgc tggcggacct
     2581 gcacagcgaa agtgcacccg accgaccgct caacggcgca cagctggtga cgggggacat
     2641 cgacctcgac gatcgcgtcg acgcgatgct ggtggcctcc ggcgtggtcg tgcacctgct
     2701 ggacatcggc gccgacgatc cgcctgcgcc gcggatgcgc gagtatcccg ggctcgctgc
     2761 tcccgacgcg ctcgcgctcg cggacgtcga cggtgagggc ccgctcgacc tcgtcagctg
     2821 ttcgtacggc ggcctccgga tcctcctcca ggcttctgac ggcgaggtgc agccgcaggt
     2881 ggcgcacgac cagttcgtcc gcagctgcgc gctgcatgtc gacccggacg accggcgagc
     2941 gaccgcggcg accgcgaccg accgcgggta cgcgctgctg cgcccggact tcgcgccagc
     3001 gctcgcgcgc acggacagct tcgagggcgg catgagcttc ggggggcgcg tcgaggccgg
     3061 ggacatcgac gccgacggcg tcgccgacat cgtcgtcgcc gaccgcccgt ggggctgggg
     3121 cgggagcacg gcggtcctgt ggggcagcgc gggcggccag gcgcgccggg ccacacgaca
     3181 ggcgggcatg ccgtttcggg tggccgagct cgcggtcgcg ccgctcgacg accggccggg
     3241 ggacgaggtc gtcatggcct ggacgacggg gaacatgtcg atcggggccg ggggggcggt
     3301 gcagatctgg acgcacgccg aggggtccct gcagcagcgc ttcacgacga atccgcggct
     3361 cgggtcgcag gtggtgggcg tcggcgtgca gcggcgcgcc ggggctccca gcgacgtgat
     3421 cctgctcgat cgatttgcgc ccggggagct cggcgtgatc gcggtgccgc gcggggacga
     3481 cggcgacttc atcgccgacg ccgatgtgtt gctgtggtcc gggccgtgga tcgacgatgc
     3541 gccggcgatg gtgatcgccg acttcgacgg tgacggctac gaggatatcg ccgtgtccta
     3601 cggagccctc gagggggtcg cgctcacgtg gggcgaccgc gagcgcacgc ccgctgtcac
     3661 gaccgtgccg atcccggtgg ccgcgcgcac gaacctcgcc agcgccgacc tcgacgacga
     3721 cggcgcggcg gagctgttcg tcggcaccga gcagggcgtg gtccgcatcg ctttcgctgg
     3781 acgcgagccg aagccgcggg aaaagctgct cgcggactcc gccgtcctca acctgacgat
     3841 caccgacgtc gacggcgacg ggatcaccga catcgtcgcc cataacagct ggtggctcag
     3901 catcaacctg cgcgcgccga cgggcattta tttcgcaaag ctgttgtttt tgccgctgtc
     3961 tcagctgcgg gccgccgaca tcgacggcga ccagatcctc gacttcatcg ggatattcgg
     4021 cggcgaagtg atcacccgcc gctcgaccgg cgcaccgggc ccgccgctct gagcggcatg
     4081 atcaagtcgg tgatgatcaa ctgcggcggc cgggcgaggc ccgcttgatc gccgcgagaa
     4141 agcgcgacag cggtacgccc gcgtcgaggg tgctgctgcc gtcgtggtcg aggcggtgga
     4201 cgagcgggtc gtcgctgata tcgtcgacgc gcaccacggc gaagtactga tgcacgtcgt
     4261 gggcgatggc gagcagcggg ccatcgtatc cgctggcgag cctctctgtg acctccgacc
     4321 acccgagcga cagcgccgcc gcgggctctc cggacacgaa ggtgcggtcc gccggccact
     4381 tcaccgcgag cagcccggag acgacgcgtg agagcgtccg cggcccggcg accgtattga
     4441 cctgccgcgt ggcgatcgca tcctccgcgg gtggtacgcc gccgagccga acgatctgcc
     4501 ggaggagaag cttcggtagg ccggaggtcg gcggtccgag cgagcggagg aagcgcgaca
     4561 gcggcatgta ccccgaataa atgctgcctg cccaggtgtc gggctcgtcg agcgccagcg
     4621 gatagacctg aggatctcgc gcgtcgccgt tggcgatcat catcgcccgg ggtttgccgc
     4681 gagatgtata cataccgatg cacagctggc gcggcacgac attttcgagg aagtgcaatg
     4741 gtacgccgcg aaattgctcg atcggcggtc caccggcagg gcccggcgcc gcgagctcga
     4801 ggtccggcca gcgcgcccgg gcgaggcgac cgagcgtgcc cggcccgaac gcggtcgtgt
     4861 cgacggtgtc gtcgaaggtg acgccgaggc gcacgagctc ggccgcgacg cgcacgggta
     4921 gctcgtccga cttcggggtg cgggcgggtc gggtgctcat cgggcccgag tatgcgcgga
     4981 tccgttcgcg ggtgtacggg tttgtcgcgg ttatcctcga tcgccacgcc cggcgggccc
     5041 gggacttcgg cccgccggcc tgccgaccgg tgtaagatcg gcgcctcatg gtcacgatcc
     5101 ttcatgtctc ggacatgcag ttcggcgcgc agcaccggtt cgggcggctc gggctggggg
     5161 gcgcggacga ggcgttcgat acgctgctgc gccgcctcac cgacgacctc gaccgcatgc
     5221 gcgacgagga ggggatcatg ccggatctcg tggcgctcac gggcgacctg gcggagtggg
     5281 cgcggccgaa ggagtacgcg gacgtgctgc agttttgcga gggcctgcgc gcgcacctgc
     5341 agctcgccgc cgatcgcctg ctggtcatcc ccggcaacca tgacgtcaac cgcgaccatt
     5401 gccagagtta cttcgcatat tgcgccggca acgacgaggc gccccaggag ccgtggtggc
     5461 ccaagtgggg cccgtacacc aatttcttcg gcaagctgta cggcgccgtc gagcgctacg
     5521 agttcaagga gctcgcgccg tatacgtggt tcgagctgcc cgagctcaag gtggtggtcg
     5581 cggggctcaa ctcgacgatg cgcgagagcc acgaggacag ggacccgggc gaccccgcga
     5641 gcatcttcgg gcattatgga tatctcgggg aggcgcagct cgactggttc ggtcgcaagc
     5701 tcgacgacgc cgcccacaag gggtggctgc gcatcggcct ggtccaccac aacgcgcagc
     5761 gcaacgcggt cgacgacgac gagaacctgc gcgacgtcgg gatgctcgtc gaccggctcg
     5821 gcgggcggct caacctgctc ctgcacggcc acacccaccg cgggacgctc ggctggctca
     5881 ggcgcgacct gccggtgatc tcgaccggca gcgccggcgt gaaggccgga cagcgccccg
     5941 aggaggtgcc caaccagtat cagatcgtgc aggtcggacc cgcgggcttt cgctgctggg
     6001 cgcgccagta cacccccgcg gctcgcaagt ggatcggtga tacacgggtg tcgcaggacg
     6061 ggagcgactg gcggcacgcg cacgaggtgg ccttcccgcg cacgagcggg acgttcggga
     6121 cccgggagtc gcgcgggcgt ccgggcgctt cgccgttcgc gagcgacgac gaggaggagg
     6181 acccgcatcg gcgcgcgcgc gaggccaatg acctggtcgc gcaggtgatg gcgtcgtgcc
     6241 gcgtgcgcga cgaggccggg caggtgcaga
//
LOCUS       DASWNJ010001685         2874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_134934, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001685 DASWNJ010000000
VERSION     DASWNJ010001685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134934"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VGB85_27990"
     CDS             <1..279
                     /locus_tag="VGB85_27990"
                     /inference="COORDINATES: protein motif:HMM:NF025189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEY0137971.1"
                     /translation="LGRKNWLFLGSDEGARVNTVFVSLLASCQLHGIEPWAYLGDLLC
                     LLPSWPRSRVRELAPAFWQQTLQQQDAQQRLAANVFRGVSLVDHAPST"
     gene            complement(339..1322)
                     /gene="tssI"
                     /locus_tag="VGB85_27995"
     CDS             complement(339..1322)
                     /gene="tssI"
                     /locus_tag="VGB85_27995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HEY0137972.1"
                     /translation="MDRTVERTSQRPLNAHAYLHLAVVVVKHGASDLADGLDHRAESF
                     DETSSCWVRVSQLSAGNGWGAMFLPRIGHEVLVDFIEGDPDRPIVVGRVYTGTNMPPY
                     PLPEQKTRSTIKSESSVGGGGFNELRFEDRKGAEEVYLHAQKDWNTDIGNNLGETVKA
                     SRTSTIGGSETVSVGGSETVSVGGSRTVSVSGSDSTSIGAVHSATISQGKPAPGVGPT
                     SYTMSDTLYTVTSGQATITLAGPDVFIDAKGTIFLDGANVQISARGGGAVVISGGPMV
                     HINPPAEPPPAVVAAAAAAEAAAAEEATGMAAGAAAALGGRGFGEGGGGAW"
     gene            1239..1442
                     /locus_tag="VGB85_28000"
     CDS             1239..1442
                     /locus_tag="VGB85_28000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137973.1"
                     /translation="MLDDDNREMEVSMGIQGTLRRSLDRAVDLDGVSQGAMKLAGEVV
                     QDGVAVTMSVTGRYTARITGTLR"
     gene            complement(1568..2353)
                     /locus_tag="VGB85_28005"
     CDS             complement(1568..2353)
                     /locus_tag="VGB85_28005"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY0137974.1"
                     /translation="MLRFTDLLGRADSVDGRAKLDLPDEWSQGRCVFGGLVAATAVRA
                     MRAHVDPQRRLRSLHVSFIAPARSGTVELAARTLRSGQNVSHVESRVSQGDELCCVAL
                     GMFAADRASPAVLASPPRPPAPEPEGLTRVPYVPGLTPAFIRHFDYRVTEAEQLPDRS
                     ELGGWCRFDEPLAPAPNWESIVGLADAWPPTIWPQLQPPPRVVSVSWSLEFVHVPASV
                     DGWWFYRANTANLVDGHAHEHATLWDPAGQVAAVSRRLLAVWS"
     gene            2526..>2874
                     /locus_tag="VGB85_28010"
     CDS             2526..>2874
                     /locus_tag="VGB85_28010"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0137975.1"
                     /translation="MLAGQVALITGGGSGIGAAIAARMAAHGAHVAICGRTQAKLDAV
                     VQAIEAGGGAALGVVADVRRPDQVEQVVQAIDARWGRLDVVVNNAAGNFLCPAVAMSA
                     NGFGAVVDIDLKGT"
BASE COUNT          420 a          983 c         1030 g          441 t
ORIGIN      
        1 ctcggccgca agaactggct cttcctcggc agcgacgagg gcgcccgcgt caacacggtc
       61 ttcgtctccc tgctcgccag ctgccagctc cacggcatcg agccctgggc ctacctcggc
      121 gacctgctgt gcctcctgcc gtcctggccc aggagccgcg tccgcgagct cgccccggcc
      181 ttctggcagc agaccctgca acagcaggac gctcagcagc gcctggccgc caacgtcttc
      241 cggggcgtct cgctcgtcga tcacgctcca tcgacgtagc ccggatcggc gccgcggtca
      301 cgacgcggtt ggccgaacgg atacggctga gcacgcgttc accacgcccc gcccccaccc
      361 tcgccgaagc ccctgccccc cagtgccgcc gccgctcctg cagccatgcc agtggcttcc
      421 tccgcggcgg cagcctcggc cgccgcggca gctgctacga ccgccggggg cggctccgca
      481 ggcgggttga tatgcaccat cggcccgccc gatatcacca ctgccccgcc accgcgcgcc
      541 gagatctgca cattcgcgcc gtcgaggaag atcgtcccct tcgcgtcgat gaacacgtcg
      601 ggtcccgcga gggtgatggt ggcctggccg gaggtcaccg tataaagagt gtcgctcatc
      661 gtgtagctgg tcgggccgac gccgggggcg ggcttgccct gggagatcgt cgcggagtgg
      721 acggcgccga tcgacgtcga gtcgctgccg ctgacggaca cggttcgcga gccgccgacg
      781 gagaccgtct ccgagccgcc gacggagacc gtctccgagc cgccgatcgt cgaggtgcgg
      841 ctggccttga ccgtctcgcc gaggttgttg ccgatgtccg tgttccagtc cttttgcgcg
      901 tggaggtaga cctcctcggc gcccttacgg tcctcgaagc gcagctcgtt gaagccgccg
      961 ccgccgaccg agctctcgga cttgatcgtg ctgcgcgtct tctgctccgg gagcgggtac
     1021 ggcggcatgt tggtgccagt gtacacgcgg ccgacgacga tcgggcggtc ggggtcgccc
     1081 tcgatgaagt cgaccaggac ctcgtggccg atgcgcggca ggaacatcgc gccccagccg
     1141 ttgcccgccg ataactggct gacacgcacc cagcacgagc tcgtctcgtc gaacgactcg
     1201 gcgcggtgat cgagtccgtc ggcgaggtcc gaggcaccat gcttgacgac gacaaccgcg
     1261 agatggaggt aagcatgggc attcagggga cgctgcgacg ttcgctcgac cgtgcggtcg
     1321 atctggacgg cgtctcgcag ggggccatga agctggctgg cgaggttgtg caggacggcg
     1381 tggccgtgac gatgtccgtt accggtcgtt acacggcgcg tattaccgga acattgcgct
     1441 gacgagcgcg gccgggggca ctccgggcgt gtacgagcga cgattccatg tcgcttggcg
     1501 ttcgtgtgtc ccgcgggcga gtgaagcttg aaaagcgtcg atggaaaagt cgccagccga
     1561 ggggctgtca cgaccagacg gcgagcaagc gccgcgatac ggcggccacc tgccctgcgg
     1621 ggtcccacag cgtcgcgtgc tcatgggcgt ggccgtcgac gaggttcgcc gtgttcgcgc
     1681 gatagaacca ccagccgtcg acggaggcgg gaacgtggac gaattccagc gaccatgaca
     1741 ccgagaccac gcggggcggt ggctgcagct gcggccagat cgtcggcggc caggcgtcgg
     1801 cgagcccgac gatcgactcc cagttcggcg cgggcgcgag cggctcgtcg aatcggcacc
     1861 agccgccgag ctcactgcgg tccgggagct gttcggcctc ggtcacgcgg tagtcgaagt
     1921 gtcgtatgaa cgccggtgtg agcccgggga cgtacgggac gcgcgtcagc ccctcgggct
     1981 cgggcgcggg cgggcgcggc gggctggcga gcacggccgg cgatgcgcgg tcggcagcga
     2041 acattccgag cgcgacgcag cagagctcgt ccccctgcga tacccgcgat tcgacgtgtg
     2101 agacgttttg tccgctccga agggtccggg ccgcgagctc gacggtcccg ctgcgggcgg
     2161 gcgcgatgaa cgagacgtgc aacgaacgca ggcgccgctg tggatcgacg tgggcgcgca
     2221 tggcccgaac cgccgtcgcc gcgaccaggc cgccgaagac acagcgcccc tgactccatt
     2281 cgtccggaag atcgagcttc gcccgtccgt ccacgctgtc ggcgcgcccc agcaggtcgg
     2341 tgaaacggag catggggggg gtggatgcgg gggtccctgt catcgtccac gtgaacgatg
     2401 ggcgccgcgg agcagacctg tcaagccgtg gagcccctgg ccggaagggt cccgggccgc
     2461 taagctcgcg gtgatcggag cgagccatgt ccagtcctca ggatgtcggt tgttttcgtg
     2521 cggatatgct tgccgggcaa gtcgcgctca tcacgggtgg cggctccggg atcggagcgg
     2581 cgatcgccgc ccggatggcc gcccacgggg cgcacgtcgc gatttgcggg cgtacgcagg
     2641 ccaagctcga cgcggtcgtc caggcgatcg aagccggcgg cggcgcggcg ctgggcgtcg
     2701 tcgccgacgt gcgtcggccc gaccaggtcg agcaggtggt ccaggcgatc gacgcgcgct
     2761 ggggtcgcct cgatgtcgtc gtcaacaacg cggccggcaa cttcctgtgt cccgcggtgg
     2821 cgatgagcgc caacggcttc ggcgccgtcg tcgacatcga cctcaagggc acgt
//
LOCUS       DASWNJ010001686         2231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_489280, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001686 DASWNJ010000000
VERSION     DASWNJ010001686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489280"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="VGB85_28015"
     CDS             complement(<1..1017)
                     /locus_tag="VGB85_28015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137976.1"
                     /translation="MTRARLSLTAPCIALLLAVGCSDGGSGLYEGDSAEGGSGVSGTA
                     TAPASSDGLYEGGDDGAAEAGDEPSADSGAAEAGEGGGEAGQLTAGEWRDLDHWQFWT
                     GLMQKQEWSAIATRWGFSTQQRYPVVVESSGAHVADATVTLLAGEQALWSARTDVHGE
                     AELFAGMFAAAPAGPYSLKVSAAGQSTVAADLPADGVPFVLPIEVAEAPAKVLDLMFV
                     IDTTGSMGDELSYLQVELADVIARVREQVGADFKFRVSVNFYRDAGDEYLVRPFPFTE
                     DIEEALSDLGKQTADGGGDWPEAVDAALVDAIDEHMWSESAVARLCFIVLDAPPHDGS
                     QVLAD"
     gene            complement(1150..>2231)
                     /locus_tag="VGB85_28020"
     CDS             complement(1150..>2231)
                     /locus_tag="VGB85_28020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0137977.1"
                     /translation="LYRGVVESVDSLIVGVDAGGTIRMWNRCAAAATGWAQEEALGRD
                     FVEQVVIPERRAECRELLAAAWRERRLADRDWPISTVDGRHREVRWSVASFTPEGDSM
                     ELLLLVGNDVTDRLELEKRAADAEAMASLATLTAGLAHEIRNPLNAALLQLELLARIG
                     SRVGDPSQRERIGSCARLVQTEIQRLSRLLEEFLGLARPRTLARRPVDVAHLCQDVAA
                     MQRPVAEAAGVTLQVHASPDLPRVLGDPPKLTQVLVNLVVNAIDAMRGASTGEIELSA
                     ERDGEGVCIRVADRGPGIDASIASQMFRPFVSTKERGTGLGLSIVKRLVDQHGGSIEL
                     GPRAGGGTVASIRLQAVREGDAREV"
BASE COUNT          298 a          856 c          777 g          300 t
ORIGIN      
        1 gtcggccagc acctggctgc cgtcgtgcgg cggggcgtcg aggacgatga agcacaggcg
       61 cgcgaccgcc gactcgctcc acatgtgctc gtcgatcgcg tcgaccagcg ccgcgtcgac
      121 cgcctcgggc cagtcgccgc cgccgtcggc cgtctgcttg ccgaggtcgg acagcgcctc
      181 ctcgatgtcc tcggtgaacg ggaacggccg cacgaggtac tcgtcgcccg cgtcgcggta
      241 gaagttcacg ctgacgcgga acttgaagtc ggcgccgacc tgctcgcgca cgcgggcgat
      301 cacgtcggcc agctcgactt gtagatacga cagctcgtcg cccatcgagc cggtcgtgtc
      361 gatgacgaac atcaggtcga gcacctttgc cggcgcctcg gcgacctcga tcggcagcac
      421 gaagggcaca ccgtcggccg ggaggtcggc ggcgaccgtg ctctgccccg ccgcgctgac
      481 cttgagcgag tacgggcccg ccggcgccgc cgcgaacatg cccgcgaaca gctccgcctc
      541 gccatgcacg tccgtgcgag ccgaccacag cgcctgctca ccggccagca gcgtcaccgt
      601 cgcgtcggcc acgtgcgcgc cgctcgactc gacgaccacc ggatagcgct gctgcgtcga
      661 gaagccccag cgcgtggcga tcgccgacca ctcctgcttt tgcatgaggc cggtccagaa
      721 ctgccagtgg tcgaggtcgc gccactcgcc cgcggtgagc tggccggcct cgccaccgcc
      781 ctcccccgcc tcggccgcgc cgctgtcggc cgagggctcg tcgccggcct cggccgcgcc
      841 gtcgtctccg ccctcgtaga gcccgtcgct ggacgccgga gcggtcgccg tgccgctcac
      901 gccgctccca ccctcggcgc tgtcgccctc gtacagaccg ctgccgccgt cactacaccc
      961 gaccgcgagg agcagcgcaa tacagggggc ggtgagcgag aggcgtgcgc gggtcatacc
     1021 cgggcagatc caaggatcgt gccagcgcac gcaggcccgc gaaacggcgt actggcggcg
     1081 cgtcgccgcc ggaccgcgac atcccgatca tggtgaggcc gggctgccct gcagcgggtg
     1141 ccctggacct cagacctcgc gggcgtcgcc ctcgcggacc gcctgcagtc ggatgctggc
     1201 gaccgtgcct ccaccggcgc gtgggccgag ctcgatcgac ccgccgtgct gatcgacgag
     1261 gcgcttgacg atcgacagcc cgaggccggt gccgcgctcc ttcgtgctga cgaacggccg
     1321 gaacatctgg ctggcgatgc tggcgtcgat gccgggcccg cggtcggcga cgcggatgca
     1381 cacgccctcg ccgtcgcgct cggccgacag ctcgatctcg ccggtgctcg ctccgcgcat
     1441 cgcgtcgatc gcgttgacga ccaggttgac gagcacctgc gtcagcttcg gcgggtcacc
     1501 gagcacccgc ggcaggtccg gcgacgcgtg cacctgcaac gtcaccccgg ccgcctcggc
     1561 gaccggtcgc tgcatcgccg cgacgtcctg gcacaggtgc gcgacgtcga cgggccggcg
     1621 cgcgagggtc cgcgggcgcg cgagcccgag gaactcctcg agcagccgcg acagccgctg
     1681 aatttcggtc tggaccagcc gggcgcacga tccaatgcgc tcgcgctggc tggggtcgcc
     1741 gacgcgcgag ccgatcctcg ccagcagctc gagctgcagc agcgccgcgt tgagcgggtt
     1801 gcggatctcg tgggcgaggc cggccgtcag cgtcgccagc gaggccatcg cctcggcgtc
     1861 cgcggcccgc ttctcgagct cgaggcggtc ggtgacgtcg ttgccgacga gcagcagcag
     1921 ctccatgctg tcgccctcgg gcgtgaacga ggcgacgctc cagcggacct cgcggtgccg
     1981 gccatcgacc gtggagatcg gccagtcgcg gtcggcgagt cggcgctcgc gccaggccgc
     2041 cgcgagcagc tcgcggcact cggcgcggcg ctccgggatc acgacttgct cgacgaagtc
     2101 gcggccgagc gcctcctcct gcgcccagcc ggtcgccgcc gccgcgcagc ggttccacat
     2161 ccggatcgtg ccgccggcgt cgacgccgac gatcagcgag tcgaccgact cgacgacgcc
     2221 gcgatacagc c
//
LOCUS       DASWNJ010001687         4880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_242020, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001687 DASWNJ010000000
VERSION     DASWNJ010001687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4880
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242020"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VGB85_28025"
     CDS             complement(<1..224)
                     /locus_tag="VGB85_28025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137978.1"
                     /translation="MNEDARGHVRRDRSFGTFAVALGVAALVTASCGGEDRRVAVYPE
                     DSSGARLNLSPAMIKQITLPDNFVLRPDEFA"
     gene            complement(221..1117)
                     /locus_tag="VGB85_28030"
     CDS             complement(221..1117)
                     /locus_tag="VGB85_28030"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0137979.1"
                     /translation="MSDEQSKPEPETAPGERMELPTWNRGRTKKRGGAEPTEDAFQVG
                     VKEVGRGAARRAPLVAVGAVLAVGAIVVAVVMYSRGQQASAAATRLLGEAATYEARAE
                     VGDPELLLGKSKRRPPAPVVKDEAERAAAVDKALAELMAQAGGTAAAQDGTLMMAARL
                     MRDGKFPEAEAAYRQFLDAAGAGHPLRWAAREGLGFAREAQNDLDSALAEFKTLAGEK
                     GVFYRDMGLWHQGRVLERQGKTDEALAIYRQYIAEFPLGDPSIAQTEVRKRLEELDPS
                     AVASAQLEPPVQVMDTPPGAAP"
     gene            1196..2377
                     /locus_tag="VGB85_28035"
     CDS             1196..2377
                     /locus_tag="VGB85_28035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137980.1"
                     /translation="MSGPLHPRRRPAAPRRPPAPRRPSTPRAALTSLAALAVLTVLPV
                     LPAAVLAAPATTPPTAPTTPATRSPAAPASPAPPSATPPSATPPFATPPAPDPASPSQ
                     SPPSPPSQPSPTAQATPAAESATTTPASQTGPRATPPPRPPAPAGEIPPAPPRDPNDA
                     PFMRPPNNQPRPTGLRTSPDLRRGYSSVRRVALTIAPLFASFRLPFLCRPAANCAGAT
                     ATPTRQHGAGIGGELDVQVIRWLWLRAQASYSWHPVDEVRVADDKMNVSVVAPSGMIR
                     ALGAGVGPVVALDLGRFIPLIEGGIGALRITTPPGAASGQLGEACLDNGVCDVGLTCG
                     GDNTCHQGLIADLYFGGAVDVLVRRHLSLGFQIRYHALLTAPGKFPVYLVGGLRLGVR
                     F"
     gene            complement(2415..4082)
                     /locus_tag="VGB85_28040"
     CDS             complement(2415..4082)
                     /locus_tag="VGB85_28040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0137981.1"
                     /translation="MSARNLLRFAPLCVSFLLASCLDHPIKGVQYDKFTVDDEVFPIA
                     VNKDVDILFVIDNSGSMAEEQALLSANFAAFIDVLEADDVKANYRIGITTTDSGNPRC
                     PSAKYTPEGGRLVLSSCIDRVAQGEFTLVEEDFSFACDDFCGKADADVEIKPTTTALD
                     PEAKPRRWLESIEGQTNVKGFDSMVEAFQCYGPQGVAGCGFESHLESMYLSLASASDK
                     SSTSNYGFIRDTAILSVVVISDEVDCSFNPATKDIFMTNKVFWNDPANDPAPSSAVCW
                     NAGVRCEGAGPSYTDCRAENFDAEGNPGAADADAVLQPIRKYVDFLQSIEDAKREIDP
                     TQEVLVSMIAGVPVGYEEGKAPLVYEDSADAKFQADFGIGPGCILPSGDPNIPDQTAI
                     PPVRERQFAEAFEVDGERNLYSICQPNYSDALDAIAEKIRDQIQPACMPSCVLDTDVS
                     TPVLEPNCQLFEVKLSDESRTGIPRCQEVNGEWVAPAGATVCFGERVDPDGTLTPSKL
                     DDMSEECTDGGLNLEFFLVRSAAAPAGTTVTATCQLSDNKPRDCPGL"
     gene            complement(4237..>4880)
                     /locus_tag="VGB85_28045"
     CDS             complement(4237..>4880)
                     /locus_tag="VGB85_28045"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0137982.1"
                     /translation="EARSALCVGLVTAARTALLIGVHTQTAAFAPRQNSLARSFMQAA
                     VPVIDSLAAREEAQQRRLAEARAEALERTNAALQSQLDTIVRQQAQIQKLSAPVLRIW
                     RGVVVVPIVGALDADQVVRLSERLLQTLSDERAQVAILELTGLDAFDAGTGDRLRSVI
                     GAANLIGARCFVTGVHPELAMAMVDHGVKGLHSFASLADGLAAAMQVLGRPAA"
BASE COUNT          632 a         1939 c         1597 g          712 t
ORIGIN      
        1 gcgaactcgt cggggcgcag cacgaagttg tcgggcagcg tgatctgctt gatcatcgcc
       61 ggcgagaggt tgagccgcgc gccgctgctg tcctccgggt agaccgcgac ccgccggtcc
      121 tcgccgccgc agctcgccgt cacgagcgcg gcgacaccga gggccaccgc gaacgtcccg
      181 aaggacctgt cccgacggac atgtccccga gcatcctcgt tcacggcgcc gctccgggcg
      241 gcgtgtccat cacctgcacc ggcggctcga gctgcgcgct ggcgacggcg ctcgggtcga
      301 gctcctcgag gcgcttgcga acctcggtct gcgcgatcga agggtcgccg agcgggaact
      361 cggcgatgta ctggcgatag atcgccagcg cctcgtcggt cttgccctgg cgctcgagca
      421 cgcgaccctg atgccacagg cccatgtcgc ggtagaacac gcccttctcg ccggccagcg
      481 tcttgaactc cgccagcgcc gagtcgaggt cgttctgggc ctcgcgcgcg aagccgaggc
      541 cctcgcgggc cgcccagcgc agcggatggc ccgccccggc cgcgtcgagg aactggcggt
      601 acgccgcctc ggcctccggg aacttgccgt cgcgcatcag ccgggccgcc atcatcagcg
      661 tgccgtcctg cgccgccgcg gtgccgccgg cctgcgccat cagctccgcg agcgccttgt
      721 cgaccgccgc ggcacgctcc gcctcgtcct tgaccaccgg cgccggcggg cggcgcttcg
      781 acttgccgag cagcagctcc gggtcaccga cctccgcgcg cgcctcgtag gtcgccgcct
      841 cgccgagcag gcgggtcgcc gccgccgaag cctgctggcc ccgcgagtac atcaccaccg
      901 ccacgacgat cgccccgacc gcgagcaccg caccgaccgc gaccagcggc gcgcgccggg
      961 ccgccccgcg gccgacctcc ttcacgccga cctgaaacgc gtcctccgtc ggctccgccc
     1021 cgccgcgctt cttggtccga ccgcggttcc aggtcggcag ctccatgcgt tcgccggggg
     1081 ccgtctcggg ctcgggcttg ctctgttcgt cgctcacgga tggcctgtct acacagcgcc
     1141 cgccgctttg tccaccggcc ccgcccaggc cccgctttga ggtaccctgt caccgatgtc
     1201 cggcccgctg cacccgcgcc gccgccccgc agccccgcgc aggccgccgg ccccgcgccg
     1261 gccgtcgacg ccgcgcgcag ccctcacgag cctcgccgcg ctcgccgtgc tcaccgtgct
     1321 ccccgtgctc cccgcggccg tgctcgccgc ccccgcgacc accccgccga ccgcacccac
     1381 cacccccgcg acgcgatcac cggcggcccc ggccagcccc gcgccaccct ccgcgactcc
     1441 accttccgcc actccaccct tcgccacccc acccgcgccc gacccggcgt cgccctcgca
     1501 atccccgccc tcgccgccct cgcagccctc gccgaccgca caggcgaccc cggccgccga
     1561 gtccgcgacc accacgcctg cgagccagac cggcccccgc gcgaccccac cgccacggcc
     1621 tcctgcgccg gccggggaga tccctccggc ccccccgcgc gacccgaacg acgccccgtt
     1681 catgcggccg ccgaacaatc aaccgcgccc gaccggcctg cgcaccagcc ccgacctgcg
     1741 ccgcggctac tcgagcgtgc gccgcgtcgc gctgacgatc gccccgctgt tcgcgtcgtt
     1801 ccgcctgccc ttcctgtgcc gcccggcggc caactgcgcc ggcgccacgg ccacgccgac
     1861 gcgccagcac ggcgcgggca tcggcggcga gctcgacgtc caggtcatcc gctggctgtg
     1921 gctgcgcgcc caggccagct actcgtggca cccggtcgac gaggtccgcg tcgccgacga
     1981 caagatgaac gtcagcgtcg tcgccccgtc cggcatgatc cgcgcgctcg gggccggcgt
     2041 cggccccgtc gtcgccctcg acctcggtcg cttcatcccg ttgatcgagg gcggcatcgg
     2101 ggccctgcgg atcaccaccc cgcccggcgc cgccagcggg cagctcggcg aggcctgcct
     2161 cgacaacggc gtgtgcgacg tcggcctcac ctgcggcggc gacaacacct gtcatcaggg
     2221 cctgatcgcc gacctctact tcggcggcgc cgtcgacgtg ctggtccgcc gccacctcag
     2281 cctcggcttc cagatccgct accacgccct gctgaccgcc ccgggcaagt tcccggtcta
     2341 cctggtcggc ggcctgcgcc tcggcgtccg cttctgacct gccccgaagg tcaggtcatc
     2401 gccggtgcgc cgcttcacag ccccgggcag tcgcgcggct tgttgtccga gagctggcag
     2461 gtcgccgtca ccgtcgtgcc ggccggggcc gcggcggagc gcacgaggaa gaactcgagg
     2521 ttgagcccgc cgtccgtgca ctcctcggac atgtcgtcga gcttgctcgg cgtcagggtc
     2581 ccgtccgggt cgacgcgctc gccgaagcac accgtcgcgc cggccggcgc gacccactcg
     2641 ccgttgacct cctggcagcg cgggatgccg gtccggctct cgtcgctgag cttgacctcg
     2701 aacagctgac aattgggctc gagcaccggc gtgctgacat cggtgtcgag cacgcagctc
     2761 ggcatgcagg ccggctggat ctggtcgcgg atcttctccg cgatcgcgtc gagcgcgtcg
     2821 ctgtagttcg gctggcagat cgagtacagg ttgcgctcgc cgtcgacctc gaaggcctcc
     2881 gcgaactgcc gctcgcgcac cggcgggatc gcggtctgat cagggatgtt cgggtcgccg
     2941 ctcggcagga tgcagccggg cccgatcccg aagtccgcct ggaacttggc gtccgccgag
     3001 tcctcgtaga ccagcggcgc cttcccctcc tcatacccga ccggcacgcc cgcgatcatc
     3061 gacaccagca cctcctgggt cgggtcgatc tcccgcttgg cgtcctcgat gctctgcagg
     3121 aaatcgacgt acttgcggat cggctgcagc accgcgtcgg cgtccgcggc ccctggattg
     3181 ccctcggcgt cgaagttctc cgcccggcag tccgtgtagc tcggcccggc cccctcgcag
     3241 cgcacgcccg cgttccagca caccgccgac gacggcgccg ggtcgttcgc cgggtcgttc
     3301 cagaacacct tgttggtcat gaagatgtcc ttggtcgccg ggttgaacga gcagtcgacc
     3361 tcgtccgaga tcacgaccac cgacaggatc gcggtgtcac ggataaaccc gtagttgctc
     3421 gtcgacgact tgtccgacgc cgacgccagc gacaaataca tgctctcgag gtgcgactcg
     3481 aacccgcagc ccgcgacccc ctgcggcccg tagcactgga acgcctcgac catcgagtcg
     3541 aagcccttga cgttggtctg gccctcgatg ctctcgagcc agcgccgcgg cttggcctcc
     3601 gggtcgagcg ccgtcgtcgt cggcttgatc tcgacgtccg cgtccgcctt gccgcaaaag
     3661 tcgtcgcacg cgaacgagaa gtcctcctcg acgagcgtga actcgccctg cgcgacccgg
     3721 tcgatgcagc tcgaaagcac cagccgcccg ccctccggcg tatacttggc gctcggacac
     3781 cgcgggttgc ccgagtcggt cgtcgtgatc ccgatgcgat agttggcctt gacgtcgtcc
     3841 gcctcgagca cgtcgatgaa ggcggcgaag ttggccgaca gcagcgcctg ctcctccgcc
     3901 atcgagcccg aattgtcgat cacgaagagg atgtcgacat ccttgttgac ggcgatcggg
     3961 aacacctcgt cgtcgaccgt gaacttgtcg tactggactc ccttgatcgg atgatcgagg
     4021 cagctggcga gcagaaacga gacacagagc ggggcgaagc gaagcaggtt acgggcggac
     4081 atggtggacc tccaccccct cccttgctcg ccctgcgcca gggcccgaac ctcatgttct
     4141 caagcacttg cgtcgcccgc cgcggactcg cgcgtgcccg aggttggcga tctcggtggc
     4201 gggtgccaac cctgttggcg ggcgcccggg cgcgactcag gcggccggtc gcccgagcac
     4261 ctgcatcgcc gccgcgagcc cgtccgccag gctggcgaag ctgtgcagcc ccttcacgcc
     4321 gtggtcgacc atcgccatcg ccagctcggg gtgcacgccg gtgacgaagc agcgcgcgcc
     4381 gatcaggttg gccgcgccga tcaccgagcg caggcggtcg ccggtgccgg cgtcgaaggc
     4441 gtcgaggccg gtgagctcga ggatcgcgac ctgggcccgc tcgtcgctca gggtctgcag
     4501 caggcgctcg ctgaggcgca cgacctggtc cgcgtcgagg gcgccgacga tcgggacgac
     4561 gacgacgccg cgccagatcc ggagcacggg cgcggagagc ttctggatct gggcctgctg
     4621 gcggacgatc gtgtcgagct gagattgcag ggccgcgttg gtgcgctcga gggcctcggc
     4681 gcgggcctcg gcgagccggc gctgctgcgc ctcctcgcgg gccgcgaggc tgtcgatcac
     4741 cggcacggcc gcctgcatga agctgcgcgc gagcgagttc tggcgcggcg cgaacgctgc
     4801 cgtctgggtg tgcacgccga tcagcagcgc cgtgcgggcc gcggtgacga ggccgacgca
     4861 cagcgccgag cgggcctccg
//
LOCUS       DASWNJ010001688         1883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_101368, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001688 DASWNJ010000000
VERSION     DASWNJ010001688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101368"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="VGB85_28050"
     CDS             complement(<1..1239)
                     /locus_tag="VGB85_28050"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0137983.1"
                     /translation="MKSAPRRAWYLASMAASLATGALLGACSLAVDFAECRNDADCVS
                     RGGEQGTCVDSRCVAPPDDGDTTTTTAPPTTGDTTTLDPPATSTGEDPTTTGPITATT
                     GTTGDEPTTGSGSSSDTGVPTCALHSECEDSLGDGYLCIESACVSVFSDECQKFVWPS
                     QGSHDKVVLLGSIVPTSPPYDVITVPLQNGVQLAIEDYNRTTDLPGGNRIAWIACDDA
                     GSAVKALAAAKHLTETLKVPAVVGPVFSEQVIAIAQAVTVPAGTFLITPSATSKAITT
                     LEDDNLVWRPIASDVYQANALADRVLQLAPKATRVAVLGKADAYGKGIISDVTKRLSK
                     LLGAGFKTFEYPDPASLTPDELANEYSAVLAGAWGAKGMHPDTFLFAGTSEVAFFVAG
                     IMNLWNSEGMLDPAPRMIVTH"
     gene            complement(1256..>1883)
                     /locus_tag="VGB85_28055"
     CDS             complement(1256..>1883)
                     /locus_tag="VGB85_28055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137984.1"
                     /translation="LFEQAHAVDPNPNYLFNIGRVYEEKRDLRAAVDYYQRFVMEPRV
                     DIGAREQAVQRLRVLKAILNETEPQTASGATATSGTATGPTTIGVPSDRAPAGPADPN
                     KSMRLGGYALLGAGGVAMIVGGVFGGLTLARRSALDDSMIVEDRRSIAHTGKSFAVIA
                     DVGLFAGAALAVTGLALVLVARKRTTSRSALVPSVGRNHAGLTWSVRF"
BASE COUNT          235 a          617 c          731 g          300 t
ORIGIN      
        1 gtgggtgacg atcatgcgcg gcgccggatc gagcatgccc tcggagttcc acaggttcat
       61 gatgccggcg acgaagaacg cgacctcgct ggtgccggcg aacaagaagg tgtccgggtg
      121 catgcccttg gcgccccagg ccccggcgag cacggcggag tactcgttgg cgagctcgtc
      181 gggggtcagg ctcgcggggt cggggtactc gaaggtcttg aagccggcgc cgagcagctt
      241 gctcaggcgc ttggtgacgt cgctgatgat gcccttgccg taggcgtcgg ccttgccgag
      301 cacggcgacg cgggtggcct tcggggcgag ctgcagcacg cggtcggcga gcgcgttggc
      361 ctgatacacg tcgctcgcga tcggccgcca cacgaggttg tcgtcctcga gggtggtgat
      421 cgccttgctc gtcgccgacg gggtgatcaa aaacgtgccc gccgggaccg tgacggcctg
      481 ggcgatggcg atcacctgct cgctaaagac cgggccgacg accgccggga ccttgagggt
      541 ctcggtcagg tgcttggccg cggccagcgc cttgaccgcg ctgccggcgt cgtcacaggc
      601 gatccaggcg atgcggttgc cgcccggcag gtcggtcgtg cggttgtagt cctcgatcgc
      661 cagctgcacg ccgttttgca gcggcacggt gatgacgtcg tagggcgggc tggtcggcac
      721 gatcgacccg agcagcacga ccttgtcgtg cgacccctgg ctcggccaca cgaacttctg
      781 gcactcgtcg gagaacacgc tgacgcacgc gctctcgatg cacaggtagc cgtcgccgag
      841 cgagtcctcg cactcgctgt gcagggcgca ggtcggcacg ccggtgtcgc tgctgctgcc
      901 cgagccggtg gtcggctcgt cgccggtcgt gccggtggtc gcggtgatcg ggccggtcgt
      961 cgtcgggtcc tcaccggtgc tggtggccgg cgggtcgagg gtcgtggtgt cgccggtggt
     1021 cggcggggcg gtggtcgtcg tcgtgtcgcc gtcgtccggg ggcgcgacgc agcggctgtc
     1081 gacgcaggtg ccctgctcgc cgccgcggct gacacagtcg gcgtcgttgc gacactcggc
     1141 gaagtcgacg gccagcgagc aggcgccgag cagcgcgccg gtggcgagcg atgcggccat
     1201 cgaggcgagg taccaggcgc ggcgcggggc ggacttcatg acggggctcc ttcgatcaga
     1261 accggacgct ccaggtcagg ccggcgtggt tgcgcccgac gctggggacg agcgcgctgc
     1321 gcgacgtggt ccgcttgcgg gcgacgagca cgagcgcgag gccggtgacc gcgagcgcgg
     1381 ccccggcgaa cagtccgacg tcggcgatga cggcgaagct cttgccggtg tgggcgatcg
     1441 atcgccggtc ctcgacgatc atgctgtcgt cgagcgcgct gcgcctggcg agcgtgagcc
     1501 cgccgaacac gccgccgacg atcatcgcca cgccgccggc gccgagcagc gcgtagccgc
     1561 cgaggcgcat gctcttgttc gggtcggccg ggccagcggg cgcgcggtcg ctcgggaccc
     1621 cgatggtcgt cggtccggtc gccgtgccgc tggtggcggt cgcgccggac gcggtctgcg
     1681 gctcggtctc gttgaggatc gccttgagca cgcgcaggcg ctggaccgcc tgctcgcgcg
     1741 cgccgatgtc gacgcggggc tccatcacga agcgctggta gtagtcgacc gcggcccgca
     1801 ggtcgcgctt ctcctcgtac acgcggccga tgttgaacag gtagttaggg ttcgggtcga
     1861 ccgcgtgggc ctgctcgaac agc
//
LOCUS       DASWNJ010001689         1613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_341693, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001689 DASWNJ010000000
VERSION     DASWNJ010001689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341693"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..698)
                     /locus_tag="VGB85_28060"
     CDS             complement(120..698)
                     /locus_tag="VGB85_28060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137985.1"
                     /translation="MTLIPEGSLRCEFDDALWTAIKWDAHAAYTHGIGKLQGELTDPV
                     SSQKRPAGTRAVDIVGVHAGALYLIEIKDFRGYEAANAYRQEDELPLEVGFKVRDTIA
                     GLVGAHASGRNAALLGPFIRALADARPVRVIAWILEDKGGSAGERRKYIAMNDTRTKQ
                     LARRLGWLTSSAWVDDPLNPYLALPGVAVRQA"
     gene            complement(695..>1613)
                     /locus_tag="VGB85_28065"
     CDS             complement(695..>1613)
                     /locus_tag="VGB85_28065"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0137986.1"
                     /translation="TVSESLRRHTRGEGLDGRQGESLEAIVTEMLRSVFLPEDIVHLV
                     RKVPDEAKTATVRLQWDNGAEFGLSLTTQGSLRVEVRGDFSGLDNAVFVPTRELLSIY
                     PGFTAAWLRRESAFDRTHFDLCVLLGLNPLRGAPDERRALIDPLERATQARVYRQNDR
                     FYLQYDEGSDIEASLAAEGHRKLGMIAYLIHNGSLTANGFLFWDEPEAGINPLLVRAT
                     ADTIVGLASAGLQTFLSTHDYILASDLSLRAHPDSMRFFALGRGEHGVVAEAGERMTD
                     LEHNAILDAFVHLHARERAAFVGAGSIEP"
BASE COUNT          262 a          517 c          565 g          269 t
ORIGIN      
        1 gtgcgggcga ggtgctcgcc cgcaaggtcg cgccgctcgg gttcctggcg cgcgagctgc
       61 tcggggagat cccggcgctg gtggcctgca tggaccggcc cgcggaggag taggcgcggt
      121 cacgcctgcc ggacggcgac gccgggcagg gccaggtacg gattgagcgg gtcatcgacc
      181 catgcgctgg aggtgagcca cccgagccgc cgcgcaagtt gctttgtccg ggtgtcgttc
      241 atggcgatgt attttcggcg ttcgccggcg ctgccaccct tatcctcgag gatccacgcg
      301 atgactcgca cgggacgagc atcggccagc gctcgaatga acgggccgag cagggcggcg
      361 tttcgcccac tcgcgtgcgc gccgaccagc ccggcgatcg tgtcgcggac cttgaacccg
      421 acctccagcg gcagctcgtc ctcctgtcgg taggcgttcg cggcctcgta tccgcgaaaa
      481 tccttgatct cgatcaagta cagcgcgccc gcatggacgc caacgatgtc gaccgcccgg
      541 gtgcccgctg gccgcttctg cgagctcacg gggtccgtga gctcgccttg tagcttcccg
      601 atcccatggg tgtatgccgc gtgggcgtcc cacttgatcg cggtccagag cgcatcgtcg
      661 aactcgcagc gcaggctacc ctcggggata agggtcatgg ctcgatgctc ccggcgccga
      721 caaaggccgc ccgctcgcgc gcgtgaagat gtacgaaggc gtcgaggatc gcgttgtgct
      781 cgaggtctgt catgcgctcg cccgcctcgg cgacgacgcc gtgctccccg cgaccgagcg
      841 cgaagaaccg catgctgtcg gggtgtgccc gcagcgacag gtcggacgcg aggatgtaat
      901 cgtgggtgct caaaaaggtc tgcaggcccg cgctggcgag accgacgatc gtgtccgccg
      961 tcgcgcgaac gagcagggga ttgatgcccg cctcgggttc gtcccagaac agaaaaccgt
     1021 tggcggtcag tgagccgttg tggatgaggt acgcgatcat gccgagcttg cgatgccctt
     1081 cggcggcgag cgaggcctcg atgtcgctgc cctcgtcata ttggaggtaa aagcggtcgt
     1141 tctggcgata tacgcgggcc tgtgtggcgc gctcaagggg gtcgatcagc gcccggcgct
     1201 cgtccggggc cccccgaaga gggttgagcc cgagcaatac gcacaggtca aagtgggtac
     1261 gatcgaaggc cgactcgcgc cgcagccaag ctgcggtgaa cccggggtag atcgagagca
     1321 gttcgcgcgt gggtacaaag acggcgttgt cgagcccgga gaagtcgccg cgcacctcga
     1381 ctctgagcga gccctgggtt gtcaatgaca gaccgaactc ggcgccgttg tcccattgaa
     1441 gccggaccgt cgcagtcttg gcctcgtcag ggactttccg aacgaggtgg acaatgtcct
     1501 cgggcagaaa cacgctgcgc agcatctcgg tgacgatggc ctcgagagac tcaccctggc
     1561 gaccatcgag accctcgccg cgggtgtggc ggcgcagcga ctcggacacc gtg
//
LOCUS       DASWNJ010001690         3761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_135026, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001690 DASWNJ010000000
VERSION     DASWNJ010001690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135026"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="VGB85_28070"
     CDS             <1..1360
                     /locus_tag="VGB85_28070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137987.1"
                     /translation="PELESLCAQLGPSELCTGNSRELILVRSAEGAIALDYFAFTADT
                     GAAFDAERVPVVKAALAAPIASLAPLSTLRGDFVAYADADALPALHEVLSVADAANAL
                     RIYPEGVQRYLAEAAALAQLRESRRLFSGARFELTVEGDTLQSTFQWEPRDDQARELL
                     TRLLTRTPAAASVPTISGLCDGSLACMRTAGFPAIAPFEELAAGVYARPESEMMRVLD
                     EAGDFAGFVVFLESWPNFLGAAQRWPREQGGRMETAMLGQALEAVGRVEGMGGSLRSL
                     HIGHVGRGNPSGDFIGYMRMHGQDLVLIRSLLGLAGLRFAPALLPQVTGKVESTPIPD
                     AGMPVSLYVVTDPGTVRAGDRDIEVGWAIVADSNDRLTWLLDQERSAAVTPAFYMELP
                     DLWQLFTTFDEARRELNFAQGWLAGRSARLAADVIDGRLRIDFQLAKTAAAVEPPTTP
                     DK"
     gene            1440..3452
                     /locus_tag="VGB85_28075"
     CDS             1440..3452
                     /locus_tag="VGB85_28075"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEY0137988.1"
                     /translation="MLAPSVPLNADMDVEPDPLEAALESLGLSAFRPGQREAIETLLR
                     ERRLLLIAPTGGGKSLSFQLPALLLPGTTVVLSPLIALMADQVQALVARGVAATFLAS
                     TLPGHEISARLSNLARGQYKLIYAAPERLGSPAFREILRKIQVPLLAIDEAHCISEWG
                     HDFRPEYLQIGELVREHSQARVLACTATATPIVRDEILARLGLGVETPQLIQGFARPN
                     LALQVREDDDRKSRERAVDALISQALGRDPRERQHPPRPAGAGALVRGTAIVYAPTRK
                     SAEQEQGRLAAQGWRCKAYHAGLDGNVRERVLDAFIGSELDVVVATNAFGMGIDRSDV
                     RAVVHLAPPSSLEAYYQEVGRAGRDGADAWGMMLIAPGDMPLRRRLIERGSDDGPPAP
                     TVVEHKWNLFLELMRWAEGGSCRHDAILRYFGDEGEALGGCGRCDVCQDLARGTGPDP
                     ETVSLIVRKALSAVARVHRRFGLAAAASLLAGRVDDRLERSGLVHTPTFGCLKEYDPP
                     WLMRLLRRCVVAGWVDFTSEDKPIALLTEPGRRVMKGEAPARLLLPPLSLGRVASSGG
                     KSTARPRGPAAEVGDEVVLDAAALALFQRLRAHRMQLATRDGVPPYVVASDRSLRDLA
                     LLRPSSREQLLLAHGIGPAKADKYGADLLAVIADHARGGASTGAAP"
     gene            3449..>3761
                     /locus_tag="VGB85_28080"
     CDS             3449..>3761
                     /locus_tag="VGB85_28080"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY0137989.1"
                     /translation="MTPAPTPTWTIYLLRCADGSLYAGIARDLEARLALHNRGAGAKY
                     TRGRGPVVLVWKRGRQTPRAARQLEIAIKALPRRDKERLIAGDDALWRRLRRRYCTPQ
                     GT"
BASE COUNT          496 a         1502 c         1259 g          504 t
ORIGIN      
        1 gcccgagctc gagagcctgt gcgcgcagct cgggccgagc gagctgtgca ccggcaacag
       61 ccgcgagctg atcctcgtgc gcagcgccga gggtgcgatc gccctcgact acttcgcgtt
      121 caccgccgac accggcgcgg ccttcgacgc cgagcgcgtg cccgtcgtca aggccgccct
      181 cgccgcgccg atcgcctcgc tcgcgccgct gtcgaccctg cgcggcgact tcgtcgccta
      241 cgccgacgcc gacgccctgc ccgcgctgca cgaggtcctc tccgtcgccg acgccgccaa
      301 cgcgctgcgc atctaccccg agggcgtgca acgctacctg gccgaggccg ccgcgctcgc
      361 gcagctgcgc gagagcaggc gcctgttctc cggcgcccgc ttcgagctca ccgtcgaggg
      421 cgacacgctg cagtccacct ttcaatggga gccgcgcgac gaccaggcgc gcgagctgct
      481 gacgcgcctg ctgacgcgaa caccggccgc cgccagcgtg ccgacgatct ccgggctgtg
      541 cgacggcagc ctcgcgtgca tgcgcaccgc cggcttcccg gcgatcgccc cgttcgagga
      601 gctcgccgcg ggcgtctacg ccaggcccga gagcgagatg atgcgcgtcc tcgacgaggc
      661 cggcgacttc gccgggttcg tcgtcttcct cgagagctgg cccaacttcc tcggcgccgc
      721 gcagcgctgg ccgcgggagc agggcggccg catggagacc gcgatgctcg gccaggcgct
      781 cgaggccgtc ggccgcgtcg agggcatggg cggcagcctg cgcagtctcc acatcgggca
      841 cgtcggccgc ggcaacccga gcggcgactt catcggctac atgcgcatgc acggtcagga
      901 cctcgtgctg atccgctcgc tgctcggcct cgcgggcctg cgcttcgccc ccgccctgct
      961 cccgcaggtg accggcaagg tcgagtcgac gccgatcccc gacgccggca tgcccgtcag
     1021 cctctacgtg gtgaccgacc ccggcaccgt ccgcgcgggc gaccgcgaca tcgaggtcgg
     1081 ctgggcgatc gtcgccgaca gcaacgaccg cctgacatgg ctgctcgacc aggagcgcag
     1141 cgccgccgtc acgccggcct tctacatgga gctgcccgat ctctggcagc tgttcaccac
     1201 cttcgacgag gcccggcgcg agctgaactt cgcccagggc tggctggccg ggcgatcggc
     1261 ccgcctcgcg gccgacgtga tcgatggccg cctgcgcatc gacttccagc tcgccaaaac
     1321 cgccgccgcc gtggagcccc cgacgacccc cgacaagtag ctcgtcaggg aatgcaggcg
     1381 ccgcgaccgc gcatgacgtt ttcgcgcggc gacccttgca cgcgacgcac ccgcggatca
     1441 tgctcgcccc ctctgtgccc ttgaacgccg acatggatgt cgagcccgac cccctcgaag
     1501 cagcgctcga gagcctcggc ctgtcggcgt ttcgccccgg tcaacgcgag gcgatcgaga
     1561 ccctgctgcg cgagcgccgg ctgctgttga tcgccccgac cggcggcggc aagagcctgt
     1621 cgttccagct cccggcgctg ctgctgcccg gcaccaccgt cgtgctctcg ccgctgatcg
     1681 cgttgatggc cgatcaggtc caggcgctgg tcgcccgcgg cgtcgcggcc acctttctcg
     1741 cctccaccct gcccggccac gagatcagcg cgcgcctctc caacctcgcg cgaggtcaat
     1801 acaagctgat ctacgccgcc ccggagcggc tcggctcgcc ggccttccgc gagatcctgc
     1861 gcaagatcca ggtcccgctg ctggcgatcg acgaggccca ctgcatcagc gagtggggcc
     1921 acgactttcg cccggagtac ctgcagatcg gcgagctggt gcgcgagcac agccaggccc
     1981 gcgtgctcgc ctgcaccgcc accgcgacgc cgatcgtgcg cgacgagatc ctcgcccgcc
     2041 tcggcctcgg cgtcgagact ccccagctga tccagggctt cgcgcgcccc aacctcgcgc
     2101 tgcaggtccg cgaggacgac gaccgcaaga gccgcgagcg ggccgtcgac gcgctgatca
     2161 gccaggcgct cggccgtgac ccgcgcgagc gccagcaccc acctcgtcct gctggggcgg
     2221 gcgcgctcgt ccgcggcacc gcgatcgtct acgccccgac ccgcaagtcc gccgaacagg
     2281 agcagggccg cctcgccgcg cagggctggc gctgcaaggc ctaccacgcc ggcctcgacg
     2341 gcaacgtgcg cgagcgggtg ctcgacgcgt tcatcggcag cgagctcgac gtcgtcgtcg
     2401 ccaccaacgc cttcggcatg ggcatcgacc gcagcgacgt ccgggccgtc gtccacctcg
     2461 cgccgccgag ctcgctcgag gcttactatc aagaggtcgg ccgcgccggt cgcgacggcg
     2521 ccgacgcgtg gggcatgatg ctgatcgccc ccggcgacat gccgctgcgc cggcgcctga
     2581 tcgagcgcgg cagcgacgac ggcccacccg ccccgacggt cgtcgagcac aagtggaacc
     2641 tgtttttgga gctgatgcgc tgggccgagg gcggctcgtg ccggcacgac gcgatcctgc
     2701 gctacttcgg cgacgagggc gaggcgctcg gcggatgcgg ccgctgcgat gtctgtcagg
     2761 acctggcccg cgggacaggt cccgatcccg agaccgtcag cctgatcgtc cgcaaggcgc
     2821 tcagcgcggt cgcccgcgtg caccggcgct tcggcctcgc cgccgccgcg agcctgctcg
     2881 ccggtcgcgt cgacgaccgc ctcgagcgct ccggtctggt ccacacgccc accttcggct
     2941 gcctcaagga gtacgacccg ccgtggctga tgcgcctgct gcgacgctgc gtcgtcgccg
     3001 gctgggtcga cttcaccagc gaggacaagc cgatcgcgct gctgactgaa cccggccgcc
     3061 gcgtcatgaa gggcgaggcc cccgcgcgcc tgctgctgcc gccgctgtcg ctcggccgcg
     3121 tcgcttcctc gggcggcaag tcgaccgcgc gcccccgcgg acccgcggcc gaggtcggcg
     3181 acgaggtcgt gctcgacgcc gccgcgctcg cgctgttcca gcgcctccgc gcccaccgca
     3241 tgcagctcgc cacgcgcgac ggcgtgcccc cgtacgtggt cgccagcgac cgcagcctgc
     3301 gcgacctcgc gctcctgcgc ccgagctcgc gagagcagct gctgctcgcc cacgggatcg
     3361 gcccggccaa ggccgacaag tacggcgccg atctgctggc cgtcatcgcc gatcacgcgc
     3421 gcggtggcgc atcgaccggg gccgcgccat gacccccgcg ccgacgccga cctggacgat
     3481 ctatctcctg cgctgcgccg acggcagcct ctacgccggc atcgcccgcg acctcgaagc
     3541 ccgcctcgcc ctgcataatc ggggcgcagg cgccaagtac acccgcgggc gcggtcccgt
     3601 ggtcctcgtg tggaaacggg gccgccagac cccgcgcgcc gcccgacaac tcgagatcgc
     3661 gatcaaggcg ctgccgcgcc gcgacaagga gcgcctgatc gccggcgatg acgcgctgtg
     3721 gcggcgcctc cggcggcgct attgcacacc ccagggcacg c
//
LOCUS       DASWNJ010001691         1718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_595766, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001691 DASWNJ010000000
VERSION     DASWNJ010001691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595766"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VGB85_28085"
     CDS             complement(<1..244)
                     /locus_tag="VGB85_28085"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY0137990.1"
                     /translation="MSNLSDPGALFQIAEPSGPAPAPRAPGRGIGIDLGTTHSLVAIA
                     PQGAAPRVLRGDGRALVPSVVSYTRALDRPLVGDEAI"
     gene            complement(241..759)
                     /gene="hscB"
                     /locus_tag="VGB85_28090"
     CDS             complement(241..759)
                     /gene="hscB"
                     /locus_tag="VGB85_28090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEY0137991.1"
                     /translation="MNAMTDADYFALLGLDRRWQVDRSALERSYLERSRQAHPDAFVG
                     QGAAAQRSALEASSRLNTAYSVLRDPVRRAEYLVKLGGVDLDSSDPVRGSPQPSQSFL
                     IQMIELRERLAEDDTDAIRDEIEARAEQAFDEAVAALERNDIPVAARELVARRYYQRF
                     LDEVADAQAEGS"
     gene            complement(797..1186)
                     /locus_tag="VGB85_28095"
     CDS             complement(797..1186)
                     /locus_tag="VGB85_28095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEY0137992.1"
                     /translation="MQASADTATPTTAPATATPGPALIVTSRAAKVMREQLARRGTPQ
                     AAIRLGIRGGGCTGYSYLFEFDDREPRASDHVVVKDGVRVHVDPKSMLLLKGTEVDFE
                     TGIRGHGFKFTNPNVKDSCGCGESITF"
     gene            complement(1198..1608)
                     /gene="iscU"
                     /locus_tag="VGB85_28100"
     CDS             complement(1198..1608)
                     /gene="iscU"
                     /locus_tag="VGB85_28100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEY0137993.1"
                     /translation="MAYSTKVIDHYEHPRNVGSLDKSDESVGTGIVGAPACGDVMKLQ
                     IKVENGVITDAKFKTYGCGSAIASSSLVTEWVKGMSIDKAMEIKNSQIAEELSLPPVK
                     IHCSVLAEDAIKAAIRDYQQKHASAGGETEAAAE"
BASE COUNT          219 a          554 c          651 g          294 t
ORIGIN      
        1 cgatcgcctc gtcgccgacg agcggccggt cgagcgccct cgtgtagctg acgaccgagg
       61 gcacgagggc ccggccgtcg ccgcgcagca cccgcggcgc ggcgccttgc ggggcgatcg
      121 ccaccaggct gtgggtggtg ccgaggtcga tgccgatgcc ccgtccgggg gctcgggggg
      181 ccggagccgg cccgctgggt tcggcgatct ggaacagcgc gccggggtca ctcaaattgc
      241 tcatgaaccc tcggcctggg cgtccgcgac ttcatcgagg aagcgttgat agtagcggcg
      301 ggcgaccaat tcgcgcgcgg ccaccgggat gtcattgcgc tcgagggcgg cgaccgcctc
      361 gtcgaaggcc tgctccgcgc gggcctcgat ctcgtcgcgg atcgcgtcgg tgtcgtcctc
      421 ggcgagtcgc tcgcgcagct cgatcatctg gatcaggaag ctctggctcg gctgcggcga
      481 gccgcgcacc gggtcgctgc tgtcgaggtc gacgccgccg agcttgacga ggtactcggc
      541 gcggcggacc gggtcgcgca ggacgctgta ggcggtgttg agccgactgc tggcctcgag
      601 cgcggagcgc tgcgccgcgg ccccttgacc gacgaaggcg tccgggtggg cctggcgcga
      661 gcgctcgagg tagctgcgct cgagcgcgct gcgatcgacc tgccagcgcc gatcgagccc
      721 gagcagcgcg aagtaatcgg cgtccgtcat cgcgttcata cctgcacttt ccgacatgtc
      781 ccgaagaccc gggagctcag aaggtgatcg actcgccgca gccgcaggag tccttgacgt
      841 tcgggttggt gaacttgaag ccgtggccgc ggatgccggt ctcgaagtcg acctcggtgc
      901 ccttgagcag cagcatgctc ttggggtcga cgtggacgcg gacgccgtcc ttgacgacga
      961 cgtggtcgct ggcccgcggc tcgcggtcgt cgaactcgaa caggtacgag tagccggtgc
     1021 agccgccgcc gcggatgccg aggcggatcg ccgcctgcgg ggtgccgcga cgcgcgagct
     1081 gctcgcgcat caccttggcg gcgcgcgagg tgacgatcag cgcgggcccc ggcgtggccg
     1141 tggcaggcgc cgtggtggga gtcgcggtgt cggcggaggc ttgcatggtc gactcgctta
     1201 ctcggcggcg gcttcggtct cgccgccggc gctcgcgtgc ttttgctggt agtcgcggat
     1261 cgcggccttg atggcgtcct cggcgagcac ggagcagtgg atcttgaccg gcgggagcga
     1321 cagctcctcg gcgatctggg agttcttgat ctccatcgcc ttgtcgatgc tcatgccctt
     1381 gacccactcg gtcaccaggc tcgagctggc gatcgcggag ccgcagccgt aggtcttgaa
     1441 cttggcgtcg gtgatgacgc cgttctccac cttgatctgc agcttcatca cgtcgccgca
     1501 ggcgggggcg ccgacgatgc cggtgccgac gctctcgtcg ctcttgtcga ggctcccgac
     1561 gttgcggggg tgctcgtagt ggtcgatgac tttggtgctg taggccatgg cgggctgctc
     1621 tcttcgcggg gggacaggat aacgactgcg gacggggttg caacgcggtc gggagcgcga
     1681 ttcagggccg gaggcccgtg aagccggatc ggggccgg
//
LOCUS       DASWNJ010001692         4172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_626102, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001692 DASWNJ010000000
VERSION     DASWNJ010001692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626102"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..538)
                     /locus_tag="VGB85_28105"
     CDS             complement(92..538)
                     /locus_tag="VGB85_28105"
                     /inference="COORDINATES: protein motif:HMM:NF012393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY0137994.1"
                     /translation="MSNKPRSKRPDDAPEIIAPDGDRPRKVSRHIQPLGPRVLVRLIK
                     TPDRSESGLYLPAGARDSHSEALLAEVVEVARTMPKAAYIDDGDDDDDDDAPDLGENV
                     SGIPVGAQVLFGKEQGVTVPWDDSLRLLSVRHVLAIVDIIPEDNLQ"
     gene            complement(560..2950)
                     /locus_tag="VGB85_28110"
     CDS             complement(560..2950)
                     /locus_tag="VGB85_28110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137995.1"
                     /translation="MERWRIVAGHVPGTLALGLLGLCVVLAIAEAVLAYRRARAVPRR
                     LRVALLALRVFGVLALLVVGLELGLSQETVRPTGPRVAILVDSSASMALADADEPGAP
                     TTARIDRLRNFWAASAPARAAWREQGLAISPRAFATRSEPLDETAAETLALTPTGPAS
                     DLAHALADLAAPDAAAADARPLAAVVVLSDGLFPGTGPEGQDPLLLAARALGVPVTTV
                     SAGAPAIRDVAVAELHAGEFAFVENVAEFGAELVAHGYPGANVDVDLRRDGAIVATTR
                     VALADDGAPVPVRFEVAPDRVGQFVYEIAVRPLRGEATLANNRRAFVVKVLRDKVRVL
                     HVAGRPDWDVRALRTLLGRDPNVELLSYYILRGVDDADREDPSAELSLIAFPTDELFK
                     EQLGSFDLVVLHNFDAVNHQVARYLPDIANYVEDGGALIVIGGDLGLATGDYPGVLGQ
                     KLAVDTRAPTGLRTEPYRPAVTEAGRRHPITAWLASAGLADWSTLPALDSYNPVQLGR
                     HAESIGAAVLLTARDEAPLLAIAEPGKGRSLVLATGATWRLGFAADLPIVDGARPYDL
                     LWLGAIRWLLRDVAVERLSLETDRPSYLVGDTVHLRATTLTPSYAPEPGVALAWEVRP
                     LGAADPPTPAPPDAPPPPTFLLSEGTWTTDALGRAGAPVPGSLPVGAYEAMAWRTGPS
                     GQVDADAPAATPPARARRVFLVQEAGQELARVDADPGEQRLRALADATGGLALVARTG
                     DSLPATLPLADLGADGPGLRVDSRRSVPLWDGPWALMLVMLGLGGEVLLRRRAGLA"
     gene            complement(3036..3842)
                     /locus_tag="VGB85_28115"
     CDS             complement(3036..3842)
                     /locus_tag="VGB85_28115"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEY0137996.1"
                     /translation="MDPTRRQWLCGAAGLGAGAVARAAAAQPAIDLFGPPDPGWTEPS
                     TVLTIDLPQLQYPGRWNPRASAMRELASELRLRTRLEPVREPSAVLATSPALFATPFL
                     YVAGEQNLPALGAAAEAQLGRFIDLGGMIVFDAADGGADPGFERDVQALLGRIVPGSA
                     LTRVARDHVVYQSFYVLDEPVGRTRAYDHALGIQEEGRLKVMLVRNDLGGALARGPDG
                     IYSHPCTPGGPEQREWAIRLAINILLYATCTDYKADRAHVETRLRNRRWR"
     gene            3939..>4172
                     /locus_tag="VGB85_28120"
     CDS             3939..>4172
                     /locus_tag="VGB85_28120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137997.1"
                     /translation="MWLMRAAGAGLIVLGVAAPLLGRVAWEGQAELRAAQAAAERGRV
                     DLEIVHLGRAARWRSPVSGHDDAALERLMAVGRE"
BASE COUNT          529 a         1331 c         1808 g          504 t
ORIGIN      
        1 acgatcggcg actgggcccg ggcagacgtc gagatcgcga gcgcgacgag ccccgcggcg
       61 acctggccct tgagacatgt cgttcgggcg atcattgcag gttgtcttcc gggatgatgt
      121 cgacgatcgc gagcacgtgg cggacggaca gcaggcgcag gctgtcgtcc cacgggacgg
      181 tgacgccctg ctccttgccg aacagcacct gcgcgccgac ggggatgccg ctgacgttct
      241 cgccgaggtc gggggcgtcg tcgtcgtcgt cgtcgtcgcc gtcgtcgatg taggcggcct
      301 tgggcatggt gcgcgcgacc tcgacgactt cggccagcag cgcctcgctg tggctgtcgc
      361 gggcgccggc gggcaggtac aggccggact cgctgcggtc gggggtcttg atcaggcgca
      421 cgagcacgcg agggccgagc ggctggatgt ggcgcgagac cttgcgcggg cgatcgccgt
      481 cgggggcgat gatctcgggg gcgtcgtcgg ggcgcttgga gcgcggcttg ttcgacatcg
      541 gccgcgactg taccgcaact caggccaggc cggcgcggcg gcgcagcagg acctcgccgc
      601 cgagcccgag catgaccagc atcagcgccc aggggccgtc ccacaggggc acgctgcggc
      661 ggctgtcgac gcgcaggccg gggccgtcgg cgccgaggtc ggcgagcggg agggtggccg
      721 gcaggctgtc gccggtacgg gcgacgagcg cgaggccgcc ggtcgcgtcg gcgagggcgc
      781 gcaggcgctg ctcaccggga tcggcgtcga cgcgcgcgag ctcctggccg gcctcctgga
      841 ccaggaagac ccggcgcgcg cgggccgggg gcgtggcggc gggggcgtcg gcgtcgacct
      901 gtccggaagg acctgtccgc caggccatgg cctcgtaggc cccgaccggc aggctgccgg
      961 gcacgggggc gccggcgcgg ccgagggcgt cggtggtcca tgtcccttcg gacaggagga
     1021 aggtcggcgg cggcggggcg tcggggggcg cgggcgtcgg agggtccgcg gcgccgagcg
     1081 gtcggacctc ccaggcgagc gcgacgccgg gctcgggggc gtagctgggg gtcagggtgg
     1141 tcgcgcgcag gtgcacggtg tcgccgacga gatagctcgg acgatctgtc tcgagggaca
     1201 ggcgctcgac ggcgacgtcg cgcagcagcc accggatcgc gccgagccac aggaggtcgt
     1261 aggggcgcgc gccgtcgacg atcgggaggt cggcggcgaa gccgaggcgc caggtcgcgc
     1321 cggtcgcgag cacgaggctg cggcccttgc cgggctcggc gatcgccagc agcggggcct
     1381 cgtcgcgcgc ggtcagcagc acggcggcgc cgatgctctc ggcgtggcgg ccgagctgga
     1441 ccgggttgta gctgtcgagc gcgggcaggg tcgaccagtc ggccaggccg gcgctggcga
     1501 gccaggcggt gatcgggtgg cggcggccgg cctcggtgac ggcggggcga tacggctcgg
     1561 tgcgcaggcc ggtcggcgcg cgggtgtcga cggcgagctt ctggccgagc acgccggggt
     1621 agtcgccggt cgccaggccg aggtcgccgc cgatgacgat cagggcgccg ccgtcctcga
     1681 cgtagttcgc gatgtcgggc aggtagcggg cgacctgatg attgacggcg tcgaagttgt
     1741 ggaggacgac gaggtcgaag gacccgagct gctctttaaa aagctcgtcg gtcgggaagg
     1801 cgatcaggct cagctcggcg gacgggtcct cgcggtcggc gtcgtcgacc ccgcggagga
     1861 tgtagtagct gagcagctcg acgttggggt cgcggccgag cagggtgcga agcgcccgga
     1921 cgtcccagtc ggggcggccg gcgacgtgga gcacgcggac cttgtcgcgc aggaccttga
     1981 cgacgaaggc gcggcggttg ttggcgaggg tggcctcgcc ccgcagcggg cgcacggcga
     2041 tctcgtagac gaactggccg acgcggtcgg gcgcgacctc gaagcggacc ggcacggggg
     2101 cgccgtcgtc ggcgagggcg acgcgggtcg tcgcgacgat cgcaccgtcg cggcgcaggt
     2161 cgacgtcgac gttggccccg gggtagccgt gggcgaccag ctcggcgccg aactcggcga
     2221 cgttctcgac gaaggcgaac tcgccggcgt gcagctcggc gaccgcgacg tcgcggatcg
     2281 caggggcgcc ggcgctgacg gtggtcacgg ggacgccgag cgccctggcg gcgagcagca
     2341 gcgggtcctg tccttccgga cctgtccccg ggaacaggcc gtccgacagc acgacgacgg
     2401 cggcgagcgg gcgcgcgtcg gcggcggcgg cgtccggggc ggcgaggtcg gcgagggcgt
     2461 gggcgaggtc ggaggcgggg ccggtgggcg tcagcgcgag ggtctcggcg gcagtctcgt
     2521 cgagcggctc gctgcgcgtg gcgaaggcgc gcggggagat cgccaggccc tgctcgcgcc
     2581 aggcggcacg tgcgggggcg gaggcggccc agaagttacg caggcgatcg atgcgggccg
     2641 tggtcggggc gccgggctcg tcggcgtcgg cgagcgccat gctggcgctg ctgtcgacga
     2701 ggatcgcgac gcgcgggccg gtcgggcgca cggtctcctg gctgaggccg agctcgaggc
     2761 cgacgacgag cagcgcgagc acgccgaaca cgcgcagggc gagcagggcg acgcgcaggc
     2821 gccggggcac cgcgcgggcg cggcggtagg cgagcacggc ctcggcgatc gccagcacga
     2881 cgcagaggcc gagcaggccg agagcgagcg tgccgggtac gtgacccgcg acgatgcgcc
     2941 agcgctccat cagtcggccg acctggcggg agggacatgt cccgggagac agatggggta
     3001 tggcatgaga gcgagcgaga gcgagtgagc gagggtcagc gccagcgacg gttgcgcagg
     3061 cgggtctcga cgtgggcgcg gtcggccttg tagtcggtgc aggtggcgta cagcaggatg
     3121 ttgatggcga ggcggatcgc ccactcgcgc tgctcggggc cgccgggggt gcaggggtgg
     3181 ctgtagatgc cgtcggggcc gcgggccagc gcgccgccga ggtcgttgcg caccagcatg
     3241 accttgaggc ggccctcctc ctggatgccg agcgcgtggt cgtaggcgcg ggtgcggccg
     3301 accggctcgt cgaggacgta aaaggattga tagacgacgt ggtcgcgggc gacgcgggtc
     3361 agcgccgagc cgggtacgat gcggccgagc agcgcctgca cgtcgcgctc gaagccgggg
     3421 tcggccccgc cgtcggcggc gtcgaacacg atcatgccgc cgaggtcgat aaagcggccg
     3481 agctgggcct ccgcggcggc gccgagggcc gggaggttct gctcgccggc gacgtagagg
     3541 aagggggtgg cgaacagggc cgggctggtg gcgagcacgg ccgagggctc gcgcacgggc
     3601 tcgaggcggg tgcgcaggcg cagctcggag gccagctcgc gcatcgcgga ggcgcgcggg
     3661 ttccagcgcc cggggtactg cagctgcggg aggtcgatcg tcagcacggt gctcggctcg
     3721 gtccacccgg gatcgggcgg gccgaacagg tcgatcgccg gctgggccgc ggcagcgcgc
     3781 gcgacggcac cggcgccgag accggccgcg ccgcaaagcc actgccttcg cgtgggatcc
     3841 atcgcgggcg cagcatagcg cagtcggccc gaggttgccg cgggggcagg tcggccttta
     3901 tgctgcggtc cttggcggga gatgggaggc ggtcgcgcgt gtggctgatg cgcgccgcgg
     3961 gcgcggggct gatcgtgctc ggggtcgcgg cgccgctgct cggccgcgtc gcgtgggagg
     4021 ggcaggccga gctgcgcgcg gcgcaggcgg ccgcggaacg cgggcgggtc gacctcgaga
     4081 tcgtgcacct cggtcgcgcg gcccgctggc gctcgcccgt gtcagggcac gacgatgcgg
     4141 cgctcgagcg gctgatggcc gtcgggcgcg ag
//
LOCUS       DASWNJ010001693         2195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_700205, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001693 DASWNJ010000000
VERSION     DASWNJ010001693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700205"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VGB85_28125"
     CDS             <1..532
                     /locus_tag="VGB85_28125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0137998.1"
                     /translation="SYVYHRMKDHPVEVAGGLGLGLHDENGAAKPAWAVWALANRIDL
                     DPPMLSCGFEDLPYVRLRRGYNAKRGHWVSSRLLPPGFTAEPAGWRLWRDHQPGTVLL
                     FECGAGEHNLLTTHPGCEGLQPRGPVGWIYTDAQPGTLPLRRCRIGNGSDHLATVDPT
                     CEGLTDEGVLGHVLAP"
     gene            complement(539..>2195)
                     /locus_tag="VGB85_28130"
     CDS             complement(539..>2195)
                     /locus_tag="VGB85_28130"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY0137999.1"
                     /translation="QIAPARAGEPHALRTVRLYGIYLARAGQHRAAASWFALGGMPVH
                     QAIELERAGEDEAAAGLWARLLASDLLAERPGEPRGHAYERALAKINFGLCLRRLAAA
                     DARGGGGGGGGPLHDRARLALAEATTAVEEVADTFESEGLRERAFDCYQLIARLGVEA
                     GAFENIAEGYLNSVRILRDDGLKLDALRLYETFVALARDRGEHHAVAAILREAADYCT
                     RAGLPYADDLRWRSAEAWIKAAGEAQGAGHPVQIVENAYLAAAEAFASVRAFRQVAEV
                     YRRLASIELPSKNRERALRLLRRLGDAPTDAPRPMPVPEFLKALPDYEEVWYVDLAEW
                     ELGGDPALVAAGVMADRRFPDYVRRHALLLVLEIERRGDVLPQLDIVRRLEQIRAYPV
                     IAALEALHRRGDPALREAVAAAMGALRFKRSFSLVSQALRDPVPGVRKAAANSVRHLI
                     FPHAFEPLRRIFEARDLPDPESARESALHAIGKIGTVEALDFLCDRLREGEHPFIKLA
                     STSFSQLSNPELLPYLRRQIEFVPAEHRAVLEDTAQHLEQRLR"
BASE COUNT          294 a          770 c          843 g          288 t
ORIGIN      
        1 gagctacgtg tatcaccgca tgaaggatca cccggtcgag gtcgcgggcg ggctcgggct
       61 cggcctgcac gacgagaacg gcgcggccaa gcccgcgtgg gcggtgtggg ccctggccaa
      121 ccggatcgac ctcgacccgc cgatgctgtc ctgcggcttc gaggatctgc cgtatgtccg
      181 cctgcgccgt ggttacaatg ccaagcgcgg ccactgggtg tcgtcgcgcc tgctgccgcc
      241 gggcttcacg gccgagcccg cgggctggcg attatggaga gatcatcagc cgggcaccgt
      301 gctgttgttc gagtgcggcg ccggcgagca caacttgctg acgacccacc cgggctgcga
      361 gggcctgcag ccgcgcggcc cggtcggatg gatctacacc gatgcgcagc cgggcacgct
      421 gccgctgcgg cgctgtcgga tcgggaacgg cagcgaccac ctcgccaccg tcgatccgac
      481 ctgcgagggc ctcaccgacg agggtgtgct cggccacgtg ctcgcgccgt gaagcgggtc
      541 agcgcaggcg ctgctcgagg tgctgcgcgg tgtcctcgag caccgcgcgg tgctcggcgg
      601 gcacgaactc gatctgccgg cgcaggtagg gcagcagctc cgggttcgag agctggctga
      661 acgaggtgct ggcgagcttg atgaatggat gctcgccttc ccgcagccgg tcgcacagga
      721 agtcgagcgc ctcgacggtg ccgatcttgc cgatcgcgtg cagcgcggac tcccgcgccg
      781 actcgggatc ggggaggtcg cgcgcctcga agatccggcg cagtggttcg aaggcgtggg
      841 ggaagatcag gtggcgcacc gagttggcgg cggccttgcg cacgcccggg accgggtcgc
      901 gcagcgcctg cgagacgagg ctgaagctgc gtttgaagcg cagcgcaccc atcgcggcgg
      961 cgaccgcctc gcgcagcgca gggtcgcccc ggcggtgcag ggcctcgagc gcggcgatca
     1021 ccgggtaggc ccggatctgc tcgagccggc gcacgatgtc gagctgcggc aagacatccc
     1081 cgcggcgctc gatctcgagg accagcagca gcgcgtgacg gcggacgtag tcggggaagc
     1141 ggcggtcggc catcacgccc gcggcgacga gcgccgggtc gccgccgagc tcccactccg
     1201 cgaggtcgac gtaccagacc tcctcgtagt cgggcagcgc cttgaggaac tcgggcaccg
     1261 gcatcggccg cggcgcgtcg gtcggggcgt cgccgaggcg tcgcagcagt cgcagcgcgc
     1321 gctcgcggtt cttgctcggc agctcgatgc tcgccaggcg gcgatagacc tcggcgacct
     1381 gccggaacgc gcgcaccgac gcgaacgcct cggccgccgc gaggtaggca ttctcgacga
     1441 tctggaccgg gtgtccggcc ccctgggcct cgccggcggc cttgatccac gcctccgcgg
     1501 agcgccagcg caggtcgtcg gcgtagggca gcccggcgcg cgtgcagtag tcggcggcct
     1561 cgcgcaggat cgccgcgacg gcgtggtgct cgccgcggtc gcgggccagc gcgacgaagg
     1621 tctcgtagag gcgcagcgcg tcgagcttga ggccgtcgtc gcgcaggatc cggacgctgt
     1681 tgaggtagcc ctcggcgatg ttctcgaaag cgccggcctc gacgccgagg cgggcgatca
     1741 gctggtagca gtcgaaggcg cgctcgcgca ggccctcgct ctcgaaggtg tcggcgacct
     1801 cctcgaccgc ggtggtcgcc tcggcgagcg cgaggcgggc gcggtcgtgc agcgggccgc
     1861 caccaccacc gccaccgcca cgcgcgtcgg cggcggcgag gcgacgcagg cacagcccga
     1921 agttgatctt cgccagcgcg cgctcgtagg cgtggccgcg cggctcgccc ggtcgctcgg
     1981 cgagcaggtc cgaggcgagc aggcgggccc acagaccggc ggcggcctcg tcctcgccgg
     2041 cgcgctcgag ctcgatcgcc tggtgcaccg gcatgccgcc gagcgcgaac cacgaggcgg
     2101 ccgcccggtg ctgaccggcg cgcgcgaggt agatgccgta cagccggacc gtgcgcagcg
     2161 cgtgcggctc accggcgcgg gccggcgcga tctgg
//
LOCUS       DASWNJ010001694         1537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_135230, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001694 DASWNJ010000000
VERSION     DASWNJ010001694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135230"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1537
                     /locus_tag="VGB85_28135"
     CDS             <1..>1537
                     /locus_tag="VGB85_28135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138000.1"
                     /translation="PLLQSWYARCFPGASPYRLYALSNLGSLLGLLSYPFALEPLLPV
                     AGQGWVWSAGFILFALGCAGTAVLVGRTAPLPIAARPHAAVPSDTPPAPGPGRMTLWF
                     GLAMIASVLLLAITSQISLEVAVIPFLWMLPLSLYLLSFVLCFEYERAYHRAVWITAL
                     LIGAGCTVAIIRKGVDAPMLLQLAAYLGTLFFACMVCHGELVRLKPDPRRLTSFYLLV
                     SAGGAAGGVFTGIVAPAIFPDLWELPLALVAACALAIGLCLRDWPRRRDLRLVARSFG
                     VVYLLGITGVLVYHAHHELDDHIHVSRGFFGVLRIDQDTGESGELRTRLRHGRIIHGL
                     QFEDPDLASMPTTYYGPGSAVGLAIRHHPRREQGQPLRLGFVGLGTGTLASYAAAGDT
                     ARFYEINPHVVALSGGTKPYFTYLRDCAGTVELAIGDARINLEREPPQRFDVLALDAF
                     SSDAIPAHLLTLEAIELYLGHLRDDTSVIAVHISNRYLDLDPVVRGLADALGLSVLRI
                     DDPE"
BASE COUNT          178 a          643 c          448 g          268 t
ORIGIN      
        1 ccgctgctgc agagctggta cgcgcgctgc ttccccggcg cgtcacctta tcgcctctac
       61 gccctctcca acctcggctc gctgctcggc ctgctcagct atcccttcgc gctcgagccc
      121 ctgctcccgg tcgccggcca gggctgggtg tggtccgccg gcttcatcct gttcgcgctc
      181 ggctgcgccg gcacggccgt gctcgtcggc cgcaccgcgc cgctgccgat cgcagcgcgg
      241 ccccacgcag ctgtcccttc ggacaccccg cccgcgcccg gccccggccg catgaccctg
      301 tggttcggcc tggcgatgat cgcctccgtg ctgctgctgg cgatcaccag tcagatctcg
      361 ctcgaggtcg ccgtcatccc gttcctgtgg atgctcccgc tgtcgctgta tctgctgagc
      421 ttcgtgctct gcttcgagta tgagcgcgcg tatcaccgcg ccgtgtggat caccgcgctg
      481 ctgatcggcg ccggctgcac ggtggcgatc atccgcaagg gcgtcgatgc gccgatgctg
      541 ctccagctcg ccgcatacct cggcaccctg ttcttcgcct gcatggtctg tcacggcgag
      601 ctcgtgcgcc tcaagcccga cccgcgccgc ctgaccagct tctacctgct cgtctccgcg
      661 ggcggggccg ccggcggcgt gttcaccggc atcgtcgccc ccgcgatctt cccggacctg
      721 tgggagctgc cgctcgcgct cgtcgccgcc tgcgccctgg cgatcggcct gtgcctgcgc
      781 gactggccgc gccgccgcga cctgcgtctg gtcgcccgca gcttcggcgt cgtttatctc
      841 ctcggcatca ccggcgtgct cgtctaccac gcgcaccacg agctcgacga ccacatccac
      901 gtctcgcgcg gcttcttcgg cgtgctgcgc atcgaccagg acaccggcga gtccggcgag
      961 ctgcgcaccc gcctgcgtca cgggcggatc attcacggcc tgcagttcga ggaccccgac
     1021 ctcgccagca tgccgaccac ctattacggc cccggcagcg ccgtcggtct ggcgatccgc
     1081 caccacccgc gacgcgagca gggccagccg ctgcgcctcg gcttcgtcgg cctcggcacc
     1141 ggcaccctcg ccagctacgc cgccgccggc gacaccgccc gcttctacga gatcaacccg
     1201 cacgtggtcg ccctgtccgg cggcaccaaa ccatatttca catacctgcg cgactgcgcc
     1261 ggcaccgtcg agctggcgat cggcgacgct cgcatcaacc tcgagcgcga gcctccgcag
     1321 cgcttcgacg tgctcgccct cgacgccttc agcagcgatg ccatccccgc ccacctgctc
     1381 accctcgagg cgatcgagct ctacctcggc cacctgcgcg acgacaccag cgtgatcgcg
     1441 gtccacatct ccaaccgcta cctcgacctc gaccccgtcg ttcgcggcct cgccgacgcc
     1501 ctcggcctct ccgtcctgcg catcgacgac cccgaga
//
LOCUS       DASWNJ010001695         1891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_562450, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001695 DASWNJ010000000
VERSION     DASWNJ010001695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562450"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1774)
                     /locus_tag="VGB85_28140"
     CDS             complement(<1..1774)
                     /locus_tag="VGB85_28140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0138001.1"
                     /translation="MQSIGFKNSWRRMCVQAGLLAGLAVAFVGGVETPAVRAAPVPCQ
                     TEGECTFKKPLFGILLDYSSSMNEPLSGNVTRWQAAVEAIEGLVNDDNGYIAESFILG
                     LIRFGHDPDPGTPGSKIAGDSSGLVDGQRLDVPWYDSAAPDKPYTECTNGDAIVAALG
                     SAGPPGGGQKTGISSWTKGAIDFAAASIAQTKADHPQDQGKRAAVLLLVTDGPWTNAN
                     GAQQLAPAGQDPKLAASALWMQQGVPTYVVALGEAKEAAFADTLAAAGGTTAAVDVAT
                     PQGFKDALGAVVAAVQHDVLTPACGAGLPRIMVLLDASSSMLNINGGMQHAPPGQGGW
                     DQARDVLAGHTSMFDLDVGGELRLVDVAYVGLAVFGHNADGEHKLVVQYGPCHEDNIA
                     WALDPETSCDKPGCTDPYAKPPITWTFKDGSLVEPMFADKTLSHMPRCDLIDNLPNAC
                     IGSATYTHLGLDLIQTNLATYKAECAAIDAVQPCNDATKFINILIADGASNSTDAQLQ
                     APLVAMYEAGVVTHVIGFGDLVNIPKATLQLEKLADWGSGNTLNYYDANNQDQLEAAL
                     KTIVLGLEIDKCCNFLDCGRDPEPS"
BASE COUNT          270 a          632 c          666 g          323 t
ORIGIN      
        1 cgctcggctc ggggtccctc ccgcagtcga ggaaattgca gcacttgtcg atctccagcc
       61 cgagcacgat cgtcttgagc gccgcctcga gctgatcctg attgttcgcg tcgtaataat
      121 tgagcgtatt gcccgagccc cagtcggcca gcttctcgag ctgcaaggtg gctttgggaa
      181 tgttgacgag gtcgccgaag ccgatcacat gggtgacgac gccggcctcg tacatcgcca
      241 ccagcggcgc ctggagctgc gcgtcggtcg agttggaggc cccgtcggcg atcaggatgt
      301 tgatgaattt cgtcgcgtcg ttgcacggct gcaccgcgtc gatcgcggcg cactcggcct
      361 tgtaggtcgc gaggttggtc tggatcaggt cgaggccgag gtgggtgtag gtggccgagc
      421 cgatgcaggc gttcggcagg ttgtcgatga ggtcgcaccg cggcatgtga ctgagggtct
      481 tgtccgcgaa catcggctcg acgagcgagc cgtccttgaa ggtccacgtg atcggcggct
      541 tggcgtaagg gtcggtgcag ccgggcttgt cgcacgaggt ctctggatcg agcgcccacg
      601 cgatgttgtc ctcgtgacac gggccatact ggacgacgag cttgtgctcg ccgtcggcgt
      661 tgtggccgaa cacggcgagg ccgacgtacg cgacgtcgac gagcctgagc tcgccgccga
      721 cgtcgaggtc gaacatcgac gtgtggccgg ccagcacgtc gcgggcctgg tcccagccgc
      781 cctgcccggg cggcgcgtgc tgcatgccgc cgttgatgtt gagcatcgac gacgacgcgt
      841 cgagcagcac catgatccgc ggcagcccgg cgccgcaggc gggggtgagc acgtcgtgct
      901 ggacggcggc gaccaccgcc ccgagcgcgt ccttgaagcc ctgcggggtc gcgacgtcga
      961 ccgccgccgt cgtgccgccc gccgccgcca gcgtgtcggc gaacgcggcc tccttggcct
     1021 cgccgagcgc caccacgtag gtcggcacgc cctgctgcat ccacagcgcg ctggcggcca
     1081 gcttcgggtc ctgcccggcg ggcgcgagct gctgggcgcc gttggcgttg gtccacgggc
     1141 cgtccgtcac gagcagcagc accgccgcgc gcttgccctg gtcctgcggg tggtcggcct
     1201 tcgtctgcgc gatggaggcc gcggcgaagt cgatcgcccc cttggtccag ctcgagatgc
     1261 cggtcttctg gccgccgccg ggcggcccgg cggagccgag cgcggcgacg atcgcgtcgc
     1321 cgttggtgca ctcggtatac ggcttgtccg gggcggcgga gtcgtaccac ggcacatcga
     1381 gccgctggcc atcgacgagc cccgagctgt caccggcgat cttgctgccg ggcgtgccgg
     1441 ggtcggggtc gtggccgaag cggatcaggc cgaggatgaa gctctcggcg atgtacccgt
     1501 tgtcgtcgtt cacgagccct tcgatcgcct cgacggccgc ttgccagcgc gtcacgttgc
     1561 cgctgagcgg ctcgttcatc gagctcgagt aatcgagcaa gatcccgaac agcggcttct
     1621 tgaaagtgca ttcaccctcg gtctggcacg gcaccggcgc ggcgcggaca gcgggggtct
     1681 ccacgccgcc gacgaacgcg accgcgaggc ccgcgagcaa cccggcctgt acacacattc
     1741 gccgccatga atttttaaag ccgatactct gcatttgctg agaccccacg caaacctata
     1801 cctgcgagcg cgtcctcgca atctccgcgt gggggacttg cccaaaagga cagggccctg
     1861 gagtcaggac tcggcctggc gccacgcggg g
//
LOCUS       DASWNJ010001696         6511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_489446, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001696 DASWNJ010000000
VERSION     DASWNJ010001696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6511
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489446"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..729
                     /locus_tag="VGB85_28145"
     CDS             130..729
                     /locus_tag="VGB85_28145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138002.1"
                     /translation="MSRFETTFRTAIANAERDVLVDQIRANPQMSLSELGKLATGELG
                     NLLKQITVADLLGAVSGGGGGGGIGGAGRGGRKGGAGEGRESGGQGRKGGGEGRESGG
                     QGRKGGGGESGGAQGGARKQQKAAQAEVDTRTAEGRAAYDEAVLAALRAAGGPQSASE
                     LTAVAGGSPLQIRTALARLIETGTVNWSGRARGTRYTPA"
     gene            933..2906
                     /gene="tkt"
                     /locus_tag="VGB85_28150"
     CDS             933..2906
                     /gene="tkt"
                     /locus_tag="VGB85_28150"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY0138003.1"
                     /translation="MLAKIVDTIKTLAIDAVQKANSGHPGMPMGAAVMGSVLWGQFLV
                     HDPKAPQWPDRDRFVLSAGHGSMLLYALLHISGYDLPMEELQRFRQWDSKTPGHPEYG
                     HTVGVEVTTGPLGTGFAAAVGMALAERFMAATYNRPGHEIVDHFTYGICSDGDLMEGI
                     AAEAASFAGHLGLGKLIFLYDDNQISIDGPTSLSFTEDVAGRFSAYGWHVRKVDGHDA
                     AAVAAALVEARAESERPSLICCRTTIGHGSPGRAGSSACHGAPLGADEITATKLALKW
                     EHAEHFHVPEDVRTSFERQRLAQAEKREAWEARFKAYQEAFPELAAQYNTHLFGTVPK
                     DILAKIPSFPPGGAMATRKAGGKVLTALVEAHPTLLGGSADLAGSNGVEMALPSQSRS
                     EPGGRTIHFGVREHAMAGVCNGLALHGGVRVFDATFLVFSDFLRGALRLCALMKLPVV
                     HVFTHDSFWVGEDGPTHQPIEQLMSLRLIPGLYVVRPADARETAAAWRLALQRGPGDG
                     PTAILLTRQDLPTMPETREAIDEGAYVLWEPNGVATADLDGILIATGSEVATALEAAR
                     ALEQQGKRVRVVSMPCWEAFLAQPAAYRDEVLPPAISRRLSVEAGTTFGWARFAAHQH
                     GLDHYGASAPGKLLAEKFGFTPEAVVRRWLEIP"
     gene            3053..5152
                     /gene="fusA"
                     /locus_tag="VGB85_28155"
     CDS             3053..5152
                     /gene="fusA"
                     /locus_tag="VGB85_28155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY0138004.1"
                     /translation="MELAKVRNIGISAHIDSGKTTLSERILYYTGRIHKIEEVKGKSG
                     VGATMDSMDLEREKGITIQSAATFVTWGDHFINLIDTPGHVDFTIEVERALSVLDGAI
                     LVLCSVSGVQSQSITVDRQMRRYNVPRLAFINKMDRSGADAFKVTRQLREKLRHNAVL
                     VTYPIGAEDRFEGAVDLLTREAMYFDGDNGEIIRREPCPPELKDAVEEYRLKLVEALG
                     DFSDEIAEVFLEGEGASVTAEQLRPVIRKATLSLELTPVFVGSAFKNKGVQHLLDGVV
                     EYLPNPTEVTNKAFEIGSGGKETEVILTSDADKPLCALAFKLEEGKYGQLTYIRIYQG
                     KITKGDSIYNTRTGKKSKVGRLVRMHADEMQDIDEATAGHIAALFGIDCASGDSFTDG
                     DVVYSMRSMFVPAPVISYAVEPLKKDSLQNFSKALNRFSKEDPTFRVHRDEESGETII
                     QGMGELHLDIYIERMKREYQVETKVGEPQVAYRETITQEVQYNYTHKKQTGGSGQYAK
                     IGGVMRPIPEDSEEKIYRFVDNVVGGKIPREYISSCDDGFRQGLARGVLIGFPVVGVE
                     MEINDGGAHAVDSSDMAFQIAARAAFKETMPKASPTILEPIMRVVVETPEEFQGGIQG
                     GLVRRRGNIVGSESSMGVSVIEAHVPLANMFGYSTELRSNSQGKAEYTMEFERYEAVP
                     RHIQDELIRKYAGNKKG"
     gene            complement(5259..>6511)
                     /locus_tag="VGB85_28160"
     CDS             complement(5259..>6511)
                     /locus_tag="VGB85_28160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138005.1"
                     /translation="PAIDTVPEGVVHTHLESAPVAMLVEWGPVAGSAIGLGMLWWWIA
                     AMYSSGGRHGRPRRIALCGVLALAIHSLGDFSLEFLGVAAPLCALAGSLSERRMIVQW
                     SARKAARAGGLALAGALALAVWAMPHSWQHRHDGEPDEPGALATSQQRADVASLRTRP
                     LDGGLHTRLAFQAVEAEDWAQARARALVATRLHPNDADPWWLLTAAEYGLGNREASAD
                     ALARTLAVVRKPLPREVVDTLLERYPGADELAARMPEALLPWTLVMESIIPVAPSYAA
                     ILAATRTQVDRQEPEVLRLQVRIALETQNPALARYHASLLRQLAPDDATSHLLLVRAL
                     RAFAVPREREIQAAIDLALTRPLGDAAEVGLLEEELIESLLREGTPAARERARELLPR
                     LLSRPGDRAALQRRNALREAVQGT"
BASE COUNT         1055 a         2230 c         2320 g          906 t
ORIGIN      
        1 cgacgaggaa agccgggagg atcgcggcga tacgttttca gggtacggcc aatgccgatc
       61 tgtgcggcca aatcgataaa tatgcactgc ggcattgacg tggcatttgc ttttgtgcac
      121 atttgccgca tgtcaagatt cgagactacg tttcgtactg cgatcgccaa tgccgagaga
      181 gatgtgctgg tcgaccagat ccgcgcaaat ccccagatga gcctcagcga gctcggcaag
      241 ctcgccaccg gtgagctagg gaatctcctc aagcagatca cggtcgccga cctgctcggc
      301 gcggtcagtg gcggcggcgg cggtggtggt atcggcggcg cgggtcgtgg cggtcgcaag
      361 ggcggcgctg gcgagggtcg tgagtccggc ggccagggtc gcaagggcgg cggcgagggt
      421 cgtgagtccg gcggccaggg tcgcaagggc ggcggcggcg agtccggtgg cgctcagggc
      481 ggcgcccgca agcagcagaa ggcggcgcag gccgaggtcg acacccgcac ggccgagggc
      541 cgggcggcct acgacgaggc cgtgctggcc gcgctgcgtg ccgctggcgg cccgcagtcg
      601 gcgtcggagc tgacggcggt cgccggcggc tcgccgctgc agatccgcac cgcgctggcg
      661 cgcctgatcg agaccggcac cgtcaactgg tcgggtcgcg cccgcggcac ccgctacacc
      721 cccgcctgaa ctacccccgc ttgaactacc cccgcttgaa ctaccccccg acgtgagccg
      781 ggactgatca ccgattcatg cctgcatgtg cgggcatgaa actcacgcgg cggcggcggg
      841 cccggggcat acccaagccc gcggccggcg tgttaggcag gccgacaatg tcggcccgcg
      901 cgtccctttc cccccgagag cttcctgcgc cggtgctggc gaagatcgtc gatacgatca
      961 agacgctggc gatcgacgcc gtacaaaagg cgaattccgg ccaccccggc atgccgatgg
     1021 gcgccgcggt gatgggcagc gtgctgtggg gccagttcct cgtgcacgac cccaaggcgc
     1081 cgcagtggcc cgaccgcgac cgcttcgtgc tcagcgcggg tcacggctcg atgctgctgt
     1141 acgcgctgct gcacatcagc ggctacgacc tgccgatgga ggagctgcag cggtttcgcc
     1201 agtgggactc gaagacgccg ggtcacccgg agtacgggca cacggtcggc gtcgaggtga
     1261 cgacgggccc gctcggcacc ggcttcgcgg cggcggtcgg catggcgctc gccgagcgct
     1321 tcatggcggc gacgtacaat cgtcccggcc acgagatcgt cgatcacttc acctacggga
     1381 tctgcagcga cggcgacctg atggagggca tcgcggccga ggcggcgagc ttcgccgggc
     1441 acctcgggct cggcaagctg atcttccttt atgatgataa tcagatctcg atcgacggcc
     1501 cgacctcgct gtcgttcacg gaggacgtcg cgggccgctt ctcggcgtac ggctggcacg
     1561 tgcgcaaggt cgacggccac gacgcggcgg cggtcgcggc ggcgctcgtc gaggcgcgcg
     1621 cggagtcgga gcgcccgagc ctgatctgct gccgcacgac gatcggccac ggcagccccg
     1681 gccgggccgg cagctccgcc tgccacggcg cgccgctcgg cgccgacgag atcacggcga
     1741 ccaagctcgc gctcaagtgg gagcacgccg agcacttcca cgtgcccgag gacgtgcgca
     1801 cgtccttcga gcgccagcgg ctcgcgcagg ccgagaagcg cgaggcctgg gaggcccgct
     1861 tcaaggccta ccaggaggcg ttccccgagc tcgcggcgca gtacaacacg cacctgttcg
     1921 ggactgtccc gaaggacatc ctggcgaaga tcccgagctt cccgccgggc ggcgcgatgg
     1981 cgacccgcaa ggccggcggc aaggtgctga cggcgctggt cgaggcccac ccgacgctgc
     2041 tcggcggctc ggccgacctc gcgggctcga acggcgtcga gatggcgctg ccgagccagt
     2101 cgcgcagcga gcccggcgga cgaacgatcc acttcggcgt gcgcgagcac gcgatggcag
     2161 gcgtgtgcaa cggcctggcg ctgcacggcg gcgtgcgggt gttcgacgcg accttcctgg
     2221 tgttctcgga cttcctgcgc ggggccctgc gcctgtgcgc gctgatgaag ctgccggtcg
     2281 tgcacgtgtt cacgcacgac agcttctggg tcggcgagga cggcccgacg caccagccga
     2341 tcgagcagct gatgagcctg cgcctgatcc ccgggctgta cgtggtgcgc ccggccgacg
     2401 cgcgcgagac ggcggcggcg tggcggctcg cgctgcagcg cggccctggc gacggcccga
     2461 cggcgatctt gctgacgcgc caggacctgc cgacgatgcc cgagacgcgc gaggcgatcg
     2521 acgagggtgc ctacgtgctc tgggagccga acggcgtcgc caccgccgac ctcgacggga
     2581 tcctgatcgc gaccggctcg gaagtggcga cggcgctgga agcggcgcgt gcgctcgagc
     2641 agcagggcaa gcgcgtgcgc gtggtctcga tgccgtgctg ggaggcgttc ctggcccagc
     2701 cggcggccta ccgcgacgag gtgctcccgc cggcgatctc ccggcggctg tcggtcgagg
     2761 cgggcacgac cttcggctgg gcccgcttcg cggcccacca gcacggcctc gaccactatg
     2821 gggcgtcggc gccgggcaag ctgctggccg agaagttcgg cttcacgccg gaggcggtcg
     2881 tgcgccgctg gctcgagatc ccctgaagtt gcagtgtgga cgatcacgca ctcgaacatc
     2941 gtccggggcg gcccgcggga cctcgctgtg gcatgctccg cgcgccaaaa gccggggtta
     3001 cccgggtttt gcaatgtcat caacgtaacg gcgaaagcaa gtagagagca ccatggaact
     3061 cgcaaaggtc cgtaatatcg ggatctccgc gcacatcgac tcgggcaaga ccaccctctc
     3121 ggagcgcatc ctgtactaca cgggtcgcat ccacaagatc gaggaggtca agggcaagtc
     3181 gggcgtgggt gccaccatgg actccatgga tctcgagcgc gagaagggca tcacgatcca
     3241 gtccgcggcg acgttcgtga cgtggggtga ccacttcatc aacctcatcg acacgcccgg
     3301 gcacgtcgac ttcacgatcg aggtcgagcg cgcgctgagc gtgctcgacg gcgcgatcct
     3361 cgtgctgtgc tcggtctccg gcgtgcagtc gcagtcgatc acggtcgacc gccagatgcg
     3421 gcgctacaac gtgccccgcc tcgcgttcat caacaagatg gaccgcagcg gcgcggacgc
     3481 gttcaaggtg acccgccagc tgcgcgagaa gctgcgtcac aacgcggtgc tcgtcaccta
     3541 cccgatcggc gccgaggacc gcttcgaggg cgcagtcgac ctgctgaccc gcgaggcgat
     3601 gtacttcgac ggcgacaacg gcgagatcat ccgtcgcgag ccgtgcccgc cggagctcaa
     3661 ggacgcggtc gaggagtacc gcctcaagct cgtcgaggcg ctcggcgact tcagcgacga
     3721 gatcgccgag gtcttcctcg agggcgaggg cgcgagcgtc acggcggagc agcttcgtcc
     3781 ggtgatccgc aaggcgaccc tgtcgctcga gctgaccccg gtgttcgtcg gctccgcgtt
     3841 caagaacaag ggcgtgcagc acctgctcga cggcgtcgtc gagtacctgc cgaacccgac
     3901 cgaggtgacc aacaaggcgt tcgagatcgg ctctggcggc aaggagaccg aggtcatcct
     3961 cacctccgac gccgacaagc cgctgtgcgc gctcgcgttc aagctcgaag agggcaagta
     4021 cggtcagctg acctacatcc gcatctacca gggcaagatc accaagggtg acagcatcta
     4081 caacacccgg accggcaaga agtccaaggt gggccgtctc gtgcgcatgc acgcggacga
     4141 gatgcaggac atcgacgagg cgacggcggg ccacatcgcc gcgctgttcg gcatcgactg
     4201 cgcctcgggc gactcgttca ccgacggcga cgtcgtgtac tcgatgcgct cgatgttcgt
     4261 gccggcgccg gtcatcagct acgccgtgga gccgctcaag aaggacagcc tgcagaactt
     4321 ctcgaaggcg ctcaaccgct tctcgaagga ggacccgacc ttccgcgtgc accgcgacga
     4381 ggagtccggc gagacgatca tccagggcat gggtgagctg cacctcgaca tctacatcga
     4441 gcgcatgaag cgcgagtacc aggtcgagac gaaggtcggc gagccgcagg tcgcgtaccg
     4501 cgagaccatc acgcaagagg tgcagtacaa ctacacccac aagaagcaga cgggtggctc
     4561 gggccagtac gcgaagatcg gcggcgtgat gcgtccgatc cccgaggact ccgaggagaa
     4621 gatctaccgc ttcgtcgaca acgtcgtcgg cggcaagatc ccgcgcgagt acatctcctc
     4681 gtgcgacgac ggcttccgtc agggcctcgc tcgcggcgtg ctgatcgggt tcccggtcgt
     4741 cggcgtcgag atggagatca acgacggtgg cgcccacgcg gtcgactcga gcgacatggc
     4801 gttccagatc gccgcccgcg cggccttcaa ggagaccatg cccaaggcct ccccgacgat
     4861 cctcgagccg atcatgcgcg tggtcgtcga gacgccggag gagttccagg gcggcatcca
     4921 gggcggcctg gtccgtcgtc gtggcaacat cgtcggctcc gagagctcga tgggcgtcag
     4981 cgtcatcgag gcccacgtgc cgctcgccaa catgttcggt tactcgaccg agctgcgctc
     5041 caactcgcag ggcaaggccg agtacacgat ggagttcgag cgctacgagg cggtcccccg
     5101 ccacatccag gacgagctga tccgcaagta cgcgggcaac aagaagggct gataaagcct
     5161 ccgtcccgcg cccgagggcc gcgtcgctcc ccacaaaacg gggacggccg gccctcgcgg
     5221 cgttcggtgc gtatggaaat gctacgaggg cgcgcggctc aggtgccctg cacggcctca
     5281 cgcagggcgt tgcgccgctg gagcgcggcg cggtcgcccg ggcgggacaa taagcgaggc
     5341 agcagctcgc gggctcgctc tctcgccgcc ggggtgccct cgcgcagcag cgactcgatc
     5401 agctcctcct cgagcaaccc gacctcggca gcgtcgccga gcggacgcgt cagcgccaga
     5461 tcgatcgcgg cctggatctc gcgctcgcgc gggacggcga aggcccgcag cgcccgcacg
     5521 agcaacaggt ggctcgtcgc gtcgtcgggc gcgagctgac gcagcagact ggcgtggtag
     5581 cgggccagcg cagggttctg cgtctcgagg gcgatgcgca cctgcaggcg caatacctcg
     5641 ggctcctggc ggtcgacctg cgtgcgcgtc gcggcgagga tcgccgcgta gctcggcgcg
     5701 accgggatga tcgactccat caccagcgtc cacggcagca gcgcctcggg catgcgcgcg
     5761 gccagctcgt cggcgcccgg ataacgctcg agcaaggtgt cgacgacctc gcgcggcagc
     5821 ggcttgcgca ccaccgcgag cgtgcgcgcg agcgcgtccg ccgaggcctc ccgattcccg
     5881 aggccatact cggcggcggt caacaaccac cacgggtcgg cgtcgttggg gtgcaggcgg
     5941 gtcgccacca gggcacgcgc gcgggcctgg gcccagtcct cggcctcgac cgcctggaac
     6001 gcgaggcgcg tgtgcaggcc gccgtcgagc gggcgcgtgc gcagcgaggc gacgtcggcc
     6061 cgctgctgcg aggtcgcgag cgcgccgggc tcgtcgggct cgccgtcgtg ccggtgctgc
     6121 cagctgtgcg gcatggccca cacggcgagc gcgagggccc ccgcgagcgc gaggccgccg
     6181 gcgcgcgcgg ccttgcgagc cgaccattgc acgatcatgc gacgctcgga cagcgagccg
     6241 gcgagggcgc acagcggcgc ggcgacgccg aggaactcga ggctgaagtc gccgaggctg
     6301 tggatcgcca gggcgagcac gccacacagc gcgatcctcc gcggccggcc gtggcgcccg
     6361 ccgctgctgt acatcgccgc gatccaccac cagagcatgc cgaggccgat cgcgctgccg
     6421 gcgacggggc cccactcgac gagcatggcg accggcgcgg actcgaggtg cgtgtgcacg
     6481 acgccctccg gcacggtgtc gatcgccggg a
//
LOCUS       DASWNJ010001697         2433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_489465, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001697 DASWNJ010000000
VERSION     DASWNJ010001697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489465"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..770
                     /locus_tag="VGB85_28165"
     CDS             162..770
                     /locus_tag="VGB85_28165"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0138006.1"
                     /translation="MRRSGIVLACFVLSGLALALPACTLGVVPRDAPPPEPAPAAEPA
                     PAAEPTEPAAGPTPPAAEARVEYRLDGNRLELPGPIVFPAGKAQLDPASDPALEHARG
                     YLAAKESITVLRIEGHADDQALSEQQALAVGRWLVAHGVDCKRLLAVGFGASKPVADA
                     STPEGKARNTRIELHNAALRGRPIGGASLEGGGQVASADLCQ"
     gene            828..>2433
                     /locus_tag="VGB85_28170"
     CDS             828..>2433
                     /locus_tag="VGB85_28170"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEY0138007.1"
                     /translation="MSATSSFVYPRFDLHERRRTLPLLRAMQERVLVCDGAMGTMIQN
                     ANLSVDDFEGNDGCNELLVRTRPDVIRKIHAAYYAAGADCVETNTFGGTRLVLNEFDL
                     GHEAYALNRTAAALAREVAQEFSTPERPRFVLGSVGPTTRLVSLGHITFDDLLANYEE
                     QIGGLIDGGADGILIETCQDILQVKCATIAANRAAHKRNVQVPILVQVTVETTGTLLV
                     GTDIAGALVVIESLPIDVIGLNCATGPDLMVEHVKHLGRHSTRLVSVQPNAGLPQNVD
                     GRAVYHLTPAELAKAHARFAGEFGVSLVGGCCGTTPEHIAAIAAAVQGIVPGPRPTTF
                     QPQLASLYGAVPLDQDSGPLLVGERTNANGSLKFKEMLLAGDFDGMAELAKEQQAEGA
                     HVLDVCVAYVGRDEVRDMTEALRRIVPAISLPIMIDTTQLDVLEAALKMIGGRPIINS
                     INLEDGEDKFDKIAYLAREYGAALVALTIDEVGMAKDAASKLACAKRMRDLLVDRHGM
                     LEADIVFDPLTFTIAQGEEDSRKLGLE"
BASE COUNT          381 a          958 c          767 g          327 t
ORIGIN      
        1 tgcagcgacg gcctcagcga cgtcgtcccc aacgaggaga tgcacacgat cgtgatcgaa
       61 aataccgccc agcaggccgt gcgcaagctc gtacaggccg ccaaccacca cggcggcccc
      121 gacaacatca gtgcgatcat cctgcgccgg gtgcaatgat catgcgccgc tctggtatcg
      181 tcctcgcttg cttcgtcctc tctggcctgg ccctcgccct gccggcctgc acgctgggcg
      241 tcgtgccccg cgacgcgccc ccgcccgagc cggcccccgc ggccgagccg gcccccgcgg
      301 ccgagccgac cgagcctgcg gccgggccca cgccgcccgc ggccgaggcg cgggtcgagt
      361 accgcctcga cggcaaccgc ctcgagctgc ccggcccgat cgtcttcccc gcgggcaagg
      421 cccagctcga ccctgcgagc gatcccgccc tcgagcacgc gcgcggctat ctggccgcca
      481 aggagtcgat caccgtcctg cgcatcgagg gccacgccga cgaccaggcg ctctcggagc
      541 aacaggccct cgcggtcggg cgctggctcg tcgcgcatgg cgtcgactgc aagcgcctgc
      601 tcgcggtcgg cttcggggcc agcaagcctg tcgctgacgc gagcaccccc gagggcaagg
      661 cccggaacac ccgcatcgag ctgcacaacg cggccctgcg cggtcggccg atcggcggcg
      721 ccagcctcga gggcggcggc caggtcgcca gcgccgacct ctgccagtag cgcccggtcg
      781 cggggtttgc atccgcggcg ccgcactgct accaaccgcg gttacgtatg tccgcgacct
      841 cctcgttcgt ctacccgcgt tttgacctgc acgagcgccg gcgcaccctg ccgctcctgc
      901 gggccatgca agagcgcgtg ctcgtgtgtg acggcgcgat ggggacgatg atccagaacg
      961 ccaacctgag cgtcgacgac ttcgagggca acgacggctg caacgagctg ctcgtgcgca
     1021 cccgcccgga tgtcatccgc aagatccacg ccgcctatta cgccgccggg gccgactgcg
     1081 tcgagaccaa caccttcggc ggcacgcggc tcgtgctcaa cgagttcgac ctcggccacg
     1141 aggcctacgc cctcaaccgc accgccgccg cgctcgcccg cgaggtcgcg caggagttct
     1201 cgaccccgga gcgcccgcgc ttcgtgctcg gctcggtcgg cccgaccacc cgcctggtct
     1261 cgctcggtca catcaccttc gacgacctgc tcgccaacta cgaggagcag atcggcggcc
     1321 tgatcgacgg cggcgccgac ggcatcctga tcgagacctg ccaggacatc ttgcaggtca
     1381 agtgcgcgac gatcgcggcc aaccgcgccg cgcacaagcg caacgtccag gtcccgatcc
     1441 tcgtgcaggt gaccgtcgag acgaccggca ccctgctcgt cggcaccgac atcgccggcg
     1501 cgctcgtcgt catcgagtcg ctcccgatcg acgtcatcgg cctcaactgc gcgaccggcc
     1561 ccgacctgat ggtcgagcac gtcaagcacc tcggccgcca ctcgacccgc ctcgtcagcg
     1621 tccagcccaa cgccggtctg ccgcagaacg tcgacggccg cgccgtttat cacctgaccc
     1681 ccgccgagct cgccaaggcc cacgcgcgct tcgccggcga gttcggcgtc tcgctcgtcg
     1741 gcggctgctg cggcaccacc cccgagcaca tcgcggcgat cgccgcggcc gtgcaaggca
     1801 tcgtgcccgg cccgcgcccc acgaccttcc agccgcagct ggcgagcctc tacggcgccg
     1861 tcccgctcga ccaggacagc ggcccgctgc tcgtcggcga gcgcaccaac gccaacggca
     1921 gcctcaaatt taaagagatg ctgctcgccg gcgacttcga cggcatggcc gagctcgcca
     1981 aggagcagca ggccgagggc gcgcacgtgc tcgacgtctg cgtcgcctac gtcggccgcg
     2041 acgaggtccg cgacatgacc gaggccctgc gccggatcgt gccggccatc tccctcccga
     2101 tcatgatcga caccacgcag ctcgacgtgc tcgaggccgc gctgaagatg atcggcgggc
     2161 gcccgatcat caactcgatc aacctcgagg acggcgagga caagttcgac aagatcgcct
     2221 acctcgcccg cgagtacggc gcggcgctcg tcgccctgac gatcgacgag gtcggcatgg
     2281 ccaaggacgc cgccagcaag ctcgcctgtg cgaaacgcat gcgcgacctg ttggtcgacc
     2341 gccacggcat gctcgaggcc gacatcgtgt tcgacccgct gaccttcacg atcgcccagg
     2401 gcgaggagga cagccgcaaa ttgggcctgg aga
//
LOCUS       DASWNJ010001698         8383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_242309, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001698 DASWNJ010000000
VERSION     DASWNJ010001698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 8383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8383
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242309"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VGB85_28175"
     CDS             <1..265
                     /locus_tag="VGB85_28175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138008.1"
                     /translation="DATIAEDLMRASLQAQEQAGVAPGILAATRFVLARSLVADPRCP
                     DAEARRSQARTLAETALAAERLVPGHTAQVDAISAWLRAQAQR"
     gene            complement(299..1567)
                     /locus_tag="VGB85_28180"
     CDS             complement(299..1567)
                     /locus_tag="VGB85_28180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138009.1"
                     /translation="MTLACALAALLVAPAAGQAAPASEGPAVEATPDDRAARSHQAAL
                     AHAGRRYAEAAREYEALHAEYHDTALLLAAARSRLAAGHNAHAVAYLSQLVASGQLTA
                     ADTQVAYGELQAAQRAVTPVTVRVQLPSALAADSPRLTAHYVSRFTDEQRPPIEFLLP
                     PGAGPVRVVILQLDPGPWRLQIAEGTLAPVDVLVDVLVQPGPPIELDLRPDEAPRGLP
                     RTQRHRLAAFLGGLGGGLLGGGAGLVAHAELVRVRPALAWPPGVCADRTDCRKEVAAA
                     VTERSLGAGLLGAGAGAMIGGLTALLRDARQRRISWIVELALGGAGVVGGGLAVAFTA
                     REFDRENVDSARPWGDPDYVAAISQRSQRHTLAAGGLGLASGLALSAATGLAYTRTYN
                     RRHPDRFGRRVRPGLGLGRAGLGVTLSGRF"
     gene            1735..3027
                     /locus_tag="VGB85_28185"
     CDS             1735..3027
                     /locus_tag="VGB85_28185"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0138010.1"
                     /translation="MSARRSVPCSFVLVAAVALGCLHAGGPESTGLDGSSSGSASSSG
                     GGSECGDGTCDPGEASSCPADCESATTGSCGDGDLDASEECDVSADAVECDADCTKPQ
                     CGDGHVNAAAGEQCDDGIENSDEYSAAKRCNATCAGFAAHCGDGTCQDADEHADDCPS
                     DCAASCGNGVTEPGETCDDGNNNTPIDTGKCDKDCTATECGDSYVNTATESCDDGNAS
                     DFDACTNECIEAKCGDGLVWEGMEACDDGNLVDADACNNACVAPRRIFITSAAFKGDL
                     KPAIGEALGVGLGDAHCQELADAAGLDGTFLAWLSDSPMRRMSPATRFDTAFLGSYQL
                     VDGTAVVVAGWADLVDGTLAHAIDLDEQGNVRSGDNVWTNTAADGTAAGASSCVTWTS
                     AKVTDKGTIGVSDAIDMTWTSATENPCSGNARLYCVEQ"
     gene            complement(3089..3373)
                     /locus_tag="VGB85_28190"
     CDS             complement(3089..3373)
                     /locus_tag="VGB85_28190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138011.1"
                     /translation="MTQSGQLGEDGIHTPSGFVSRRRKDGLVDPELVDAQLRRQRDLV
                     LRKTCGTRIADGGGGVAAIAAHLRHKDMQTANRYIDRTGASARAINALES"
     gene            3628..4692
                     /locus_tag="VGB85_28195"
     CDS             3628..4692
                     /locus_tag="VGB85_28195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138012.1"
                     /translation="MPACQGPQADTSPFTTTPPLMTSAAMASSDASSGSTSGMGPSAS
                     STTSDSSSTSSSSSEGASTTLIWDVGSGRDVGDGKPVGCKGKIDFLFVISRYGGMNWF
                     QTQLLDAFPKFIDTITSKFADFDYHIMVVHADEYWGLSYCDDQCPTPAEEPCGLKDYP
                     CYYTPTHCDTQLGAGEVFPAGGKAANKLCPIDGGRRYMIKGQTDLESTFACAAQVGVS
                     GDMRMGQAMARAVQPWMNDEGSCNDGFLREEALLMVTLITNAYDQEDGAASSQVGTPA
                     TWADAVREAKHGDLDSVVMLGIIPTEGPGCKEIDRLCQMLEMFPFALNRDSWTDNYGP
                     FFEEAAGLVETACADFIPPG"
     gene            complement(4717..5619)
                     /locus_tag="VGB85_28200"
     CDS             complement(4717..5619)
                     /locus_tag="VGB85_28200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138013.1"
                     /translation="MDLKIDDQGVLFVVFHQSSNGSTRWRLMKIPAWGESPQHMELGA
                     KDETAVALAHHDSGAVAVCGTAPTGQGDKLDAMARIVRPGLATEPWTVDYLDPDIPQL
                     HRFAEQTQDCVFVGDTLTLVGEAHGAHGVEMQERDRLFILRLDTTAKTAGWNVPSPGD
                     QPQSGAQAVDTDDQANLFVAAYTCDDNCKPAADLRIYDDEDTLKWQAALGSFPTKQFA
                     VQDLVWSPAGYAVVATGGLKGGEAAFTVRAFSTSQVQALWTYTHNDLQMLQVAFALAI
                     GDYGEVYAGGLGANGFPAVAFIWG"
     gene            5572..8124
                     /locus_tag="VGB85_28205"
     CDS             5572..8124
                     /locus_tag="VGB85_28205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138014.1"
                     /translation="MEDDEQHALVVDLEVHRRTGRFGEHVHDVGQRPEPILAAHAQVA
                     VLLALRRVVAELDRLTRRQCGHGDDLARADQVIGLPLLLGAGGRQGDLIGRRDRESID
                     CILIRGQKGGVHLLCHGNKDADQYGTIEVEGPGKKKPSKKKGWYKATPQYGPRQEPPG
                     PAARANPMYVRELQEDLIWLGYFSTSRGSPQVGVFDVYTLGAVLGFKQDLIDVYGIGT
                     STTALPVAPGSVRAGQFTTPIWSQDAFISPVRIVLDWRKALLGGKKQPGVKSTIQALA
                     ARLNPKKPPTKPKERHAAHTAVTGMLAALDGFMAHWPRLAPLEQVTKPFLPFAPTAAS
                     KTVEQLASVPKDKQPKHADADALAADPVWSSTEYNACAKNREYFTVDLRDLLGSLGAA
                     LADIDASRGGLATFSPPAELAATWETTRSSAIRTLDRLEKLLGLVRFWTLDLPTQITA
                     WLAHIREMGTVDQPTAVYLKALREGGKIGPNKRPAYQLQALAQTDDFTRPDGGAIFLR
                     DECTGRKPNKNGKDATTMPEIVALQFFGNESGLKFTHGLAPFNTRTEDKAKRLPLLGI
                     DTNAHRKGSFDAVFHAGGEWYWSRGWGVGQATERDGKLDGVALRRGLPVMPADADSVQ
                     HPKPYIDREESVKDAMARKILAKYNARTDRRDCTFGETGDGKYYDCHRCLKRFVDDGL
                     VGSGEYGRGGVFVPKARGSIGSVKKASSFFVDLERYTPFARGDAGVENPLAAGQFENY
                     FAASLPEADPNVAAVLRSLKGKQTLAGAVKSTAKAAGVPAKELMAAVQAHVDVRSQFP
                     CSWLQVRIQYAGSGEQAFGSLIDLFKVVGALDTKNETMIKHIKEASELRRKA"
     gene            8130..>8383
                     /locus_tag="VGB85_28210"
     CDS             8130..>8383
                     /locus_tag="VGB85_28210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138015.1"
                     /translation="MHINLTPGIIVVLTACGPGQQSQEGPRPVSDPTPVVQPASPEPS
                     AAPKCADEPGMVYIAGGEHLATHDNKRYSVSPFWIDRTEVT"
BASE COUNT         1410 a         2855 c         2879 g         1239 t
ORIGIN      
        1 cgacgcgacg atcgccgagg acctgatgcg cgcctcgctg caggcccagg agcaggccgg
       61 cgtcgcgccc ggcatcctcg cggcgacccg cttcgtgctc gccaggagcc tcgtcgccga
      121 cccgcgctgc cccgacgcgg aggctcgccg gtcgcaggcg cgtacgctcg ccgagaccgc
      181 cctggccgcc gagcgcctgg tgcccggcca caccgcgcaa gtcgatgcga tctccgcctg
      241 gctgcgcgcg caggcccagc gatagtgcgt agattttaat atctacgtac tttgatcctc
      301 agaaccgacc cgagagcgtg accccgagcc ccgccctgcc gagcccgagg ccgggccgca
      361 cgcgccggcc gaagcggtcg gggtgcctgc ggttgtacgt gcgggtatag gcgagtccgg
      421 tcgcggcgct cagggccagg ccgctggcga gcccgaggcc gccggcggcg agcgtgtgcc
      481 gctggcttcg ctgggagatc gcagcgacat agtcggggtc gccccagggg cgcgcgctgt
      541 cgacgttctc gcggtcgaac tcgcgggccg tgaacgcgac cgcgaggccg cccccgacca
      601 cccccgcgcc gccgagcgcg agctcgacga tccacgagat ccggcgctgg cgagcgtcgc
      661 ggagcagggc ggtcaatcct ccgatcatcg ccccggcccc ggcgccgagc aggcccgcgc
      721 cgaggctgcg ctcggtcacc gcggcggcga cctccttgcg acagtcagtg cggtcggcac
      781 acacgccggg cggccacgcg agcgccggcc gcacccggac gagctcggcg tgcgcgacca
      841 gcccggcacc accgccgagc aacccaccgc cgaggccgcc caaaaacgcc gccagccggt
      901 gtcgctgggt ccgcggcagc ccgcgcggcg cctcgtccgg gcgcaggtcg agctcgatcg
      961 gcgggcccgg ctgcacgagc acgtcgacga gcacgtcgac cggcgcgagc gtgccctcgg
     1021 cgatctgcag gcgccacggc ccggggtcga gctgcaagat caccacgcgc accggtccgg
     1081 cgcccggcgg cagcaggaac tcgatcggcg ggcgctgctc gtccgtgaag cgcgacacgt
     1141 aatgcgccgt cagccgcgga gagtcggccg ccagcgccga tggcagttgt acacgcaccg
     1201 tcaccggcgt caccgcgcgc tgcgccgcct gcaattcacc atatgccacc tgcgtgtcgg
     1261 cggccgtcaa ctgcccgctc gccaccaatt gactgagata cgcgaccgcg tgcgcgttgt
     1321 ggccggccgc caggcggctg cgcgccgccg ccagcagcag cgccgtgtcg tggtactcgg
     1381 cgtgcagcgc ctcgtattcg cgggccgcct cggcgtagcg ccggcccgcg tgggcgagcg
     1441 ccgcctgatg gctgcgcgcc gccctgtcgt ccggcgtcgc ctcgaccgcg gggccctcgc
     1501 tcgcgggcgc ggcctgcccc gcggcgggcg ccacgagcag ggcagcgagg gcacaggcga
     1561 gggtcatgcg ggcactataa acggggccgg acgcgctgac cagccgcgcc tcggcggggc
     1621 atacagcatg ccgccggtgg accccggccg agaccagctg gttcatggga cagcccgggg
     1681 tttcctcgcc ggcggtggct tgcgcgctgc cgtggcatgt aggacgctgg cccgatgtca
     1741 gcgcgccgaa gcgtcccgtg ttcattcgtc ctcgtcgccg cggtcgcgct cgggtgtctg
     1801 cacgccggtg gccccgagag cacgggtctc gacggcagca gcagcggcag cgccagcagc
     1861 agcggcggcg gctccgagtg cggcgacggc acctgcgacc ccggcgaggc gagcagctgt
     1921 cccgccgact gcgagtcggc gaccaccggg tcgtgtggcg acggcgacct cgacgcctcc
     1981 gaggagtgcg acgtgtcggc cgacgccgtc gaatgcgacg ccgactgcac caagccgcag
     2041 tgcggcgacg gccacgtcaa cgccgcggcc ggcgagcagt gtgacgacgg catcgagaac
     2101 agcgacgagt actcggccgc caagcgctgc aacgccacgt gcgcaggctt cgccgcccac
     2161 tgcggggacg gcacctgcca ggacgccgac gagcacgccg acgactgccc gagcgactgc
     2221 gcggcgagct gcggcaacgg cgtgaccgag cccggcgaga cctgcgacga cggcaacaac
     2281 aacacaccga tcgacaccgg caagtgtgac aaggattgca ccgcgacgga gtgcggagat
     2341 tcgtatgtca ataccgcgac cgagtcgtgc gacgacggca acgccagcga cttcgacgcg
     2401 tgcacgaacg agtgcatcga ggccaagtgt ggcgacggcc tggtgtggga gggcatggag
     2461 gcgtgcgacg acggcaacct ggtcgacgcc gacgcctgca acaacgcctg cgtcgcgccg
     2521 cgccggatct tcatcaccag cgcggcgttc aagggcgacc tcaagccggc gatcggtgag
     2581 gcgctcggcg tcgggctggg cgacgcgcac tgccaggagc tcgccgacgc ggccggcctc
     2641 gacggcacct tcctcgcctg gctcagcgac agtcccatgc gccgcatgag cccggccacc
     2701 cgcttcgaca ccgccttcct cggcagctat caattggtcg acggcaccgc ggtggtcgtc
     2761 gccggctggg ccgacctcgt cgacggcacc ctcgcccacg cgatcgacct cgacgagcag
     2821 ggcaacgttc gctcgggcga caatgtatgg accaacaccg ccgccgatgg caccgccgcc
     2881 ggcgcgtcga gctgtgtcac ctggacctcg gcgaaggtga ccgacaaggg cacgatcggc
     2941 gtctcggacg cgatcgacat gacctggacg agcgcgaccg aaaatccgtg cagcggcaac
     3001 gcgcggctgt actgcgtcga gcagtgaggg tctggctaat tgatcttatt cttacgctaa
     3061 gttagccgag ggcgtgatca cgcctccatc agctctcgag cgcgttgatc gcgcgggcac
     3121 tggcaccggt tcggtcgatg taccggttcg cggtctgcat gtccttgtgg cgcaggtggg
     3181 cggcgatggc cgccacaccg ccgccgccgt ccgcgatgcg ggtgccgcag gtcttgcgga
     3241 gtacaagatc gcgctgacga cggagctgag cgtcgaccaa ctcggggtcg acgagcccgt
     3301 ctttgcggcg gcgcgatacg aagcctgacg gcgtgtggat gccgtcttcg ccgagctgcc
     3361 ccgactgtgt caatgaatat acattccccg gcaaccgcgt attcggtgag gtcgacctgc
     3421 cagaggtcct cgaggccctc gaggccggca ccctgggcat gatcgggggt gaccaccacg
     3481 ccgatggggc cattggcggt gatctgcggg gcgggccccg ctgcgcttga accttcggcg
     3541 ccaagcgcga ttgcgcgcgg ccgtaatgtg gggtagcgtc cgtctatgca gccaccgatc
     3601 cgggctctct cgctagtgct ctgcactgtg cctgcctgcc agggcccgca ggccgatacg
     3661 tcgccgttca cgacgacgcc gccgctgatg acgtcggccg ccatggccag cagtgacgcg
     3721 agttcaggca gcacgagcgg catggggccg agcgccagct cgaccaccag cgattcatcg
     3781 agcacctcga gcagcagcag cgagggcgcg tcgaccacgc tgatctggga cgtcggctcc
     3841 ggccgagacg tcggtgacgg aaagccggtg ggctgcaagg ggaagatcga cttcctgttc
     3901 gttatctccc gttatggtgg catgaactgg ttccagacgc agctcctgga cgctttcccc
     3961 aaattcatcg acacgatcac ctcaaagttc gctgatttcg actaccacat catggtcgtc
     4021 catgccgacg agtattgggg cctgagctac tgcgacgacc agtgtccgac gcctgccgag
     4081 gagccgtgcg ggttgaaaga ctacccctgc tactatacgc cgacccactg cgacacgcag
     4141 ctcggcgcgg gcgaggtgtt cccggcaggg ggcaaggcgg cgaacaaact gtgcccgatc
     4201 gacggtggcc gccgctacat gatcaaaggc cagaccgacc tcgagagcac cttcgcttgc
     4261 gccgcgcagg tcggggtgag cggtgacatg cgaatgggcc aggcgatggc gagagcggtc
     4321 cagccgtgga tgaacgacga gggcagctgc aacgacggat tccttcgcga agaagcgctg
     4381 ctcatggtca cgctgatcac caacgcatac gaccaggagg acggcgccgc tagctcccag
     4441 gtaggcacgc cggccacgtg ggcggacgcc gtgcgcgagg ccaaacacgg tgacctggat
     4501 tcggtcgtga tgcttggaat catccccacg gagggtcccg gctgcaaaga gatcgaccgt
     4561 ctctgtcaga tgctcgaaat gtttccgttc gcgctcaaca gggacagctg gaccgacaat
     4621 tacgggccgt tcttcgagga ggcagccggg ctcgtcgaga cagcctgcgc cgacttcatc
     4681 ccgcccggat agcggcgctc ggcgcccggc cgcccttcag ccccagataa aggcaacagc
     4741 ggggaatccg ttcgccccca acccgcccgc atagacctcg ccgtagtcgc cgatcgccag
     4801 cgcgaaggcg acttgcagca tctggaggtc attgtgggtg taggtccaga gcgcctgcac
     4861 ctggctcgtc gagaacgcac gcacggtgaa tgccgcctcg ccacccttga gcccgccggt
     4921 cgccaccacg gcgtaacccg ccgggctcca cacaagatcc tgcacggcga actgtttggt
     4981 agggaagctc ccgagggccg cctgccactt cagggtgtct tcgtcatcgt agatgcgcag
     5041 gtctgccgca ggcttgcagt tgtcgtcgca ggtataggcg gcgacaaaga ggttcgcctg
     5101 gtcatctgtg tcgaccgcct gagcgccgct ctggggctgg tcacccggcg agggcacgtt
     5161 ccatccggcc gtcttggcag tggtgtcgag gcggaggatg aagaggcgat ctcgctcctg
     5221 catctccacg ccgtgggcgc cgtgtgcctc gccaacgagg gtgagggtgt cgccgacgaa
     5281 cacgcagtcc tgcgtctgct cggcgaaccg gtgcaattgt ggtatgtcgg gatccaggta
     5341 gtcgaccgtc cacggctcgg tcgccagacc tggccggacg atccgcgcca tcgcgtcgag
     5401 cttgtcgccc tgcccggtcg gagctgtgcc gcagaccgcg actgccccgg agtcgtggtg
     5461 tgccagggcg accgccgtct cgtctttggc gccgagctcc atgtgttgtg ggctctcgcc
     5521 ccacgccggg atcttcatca agcgccagcg ggtgctgccg ttactggact gatggaagac
     5581 gacgaacagc acgccctggt cgtcgatctt gaggtccacc gccgcacagg gcgtttcggg
     5641 gagcacgtcc acgacgtcgg ccaacgccca gaacccatcc ttgccgcgca tgcgcaggta
     5701 gcagtgctgc tcgccctccg gcgagtggtt gccgaactcg acaggctcac ccgtcggcaa
     5761 tgtggccatg gcgacgacct ggcccgggcc gatcaggtca ttggtctccc acttcttctc
     5821 ggtgccgggg gtcggcaagg cgatctcata gggcgccggg accgtgagtc catcgattgt
     5881 attcttataa ggggtcagaa gggcggagtg catctcctct gccatggcaa caaggacgcc
     5941 gatcagtatg gaacgatcga ggtcgagggc ccggggaaga agaagccctc caagaagaag
     6001 ggctggtaca aggcgactcc ccagtacggg cccaggcagg agccgccggg gccggcggcg
     6061 cgcgcgaacc ccatgtatgt ccgcgagctg caggaggact tgatctggct cggctacttc
     6121 tcgacgagcc gcggctctcc gcaagtcggt gtgttcgacg tgtacacact gggagcggtg
     6181 ctcggattca agcaggacct gatcgacgtc tatggtatcg gcacgagcac gacggcgctg
     6241 cccgtcgcgc ccggtagcgt gcgggccggt cagttcacga cgccgatctg gtcccaggat
     6301 gcctttatct caccggtccg gatcgtgctc gactggcgaa aggcgctgct cggcggcaag
     6361 aagcagccag gcgtcaagtc gacgatccag gcgctggccg cccgactcaa cccgaagaag
     6421 ccgcccacga agcccaagga gcgccacgcg gcgcatacgg cggtgaccgg gatgctggcc
     6481 gcgctggacg gattcatggc gcactggcca cgcctggcgc cgctcgagca ggtcacgaag
     6541 ccgttcctcc cgttcgcgcc gacggcggcg agcaagacgg tcgagcagct cgcgtccgtc
     6601 ccgaaggaca aacagccgaa gcacgcggac gcggacgcgc tggcagctga ccccgtctgg
     6661 tcgtccacgg agtacaacgc ctgcgccaag aaccgcgaat atttcacggt cgacctgcgc
     6721 gacctgctcg gcagcctcgg cgccgcgctg gccgacatcg atgcctcgcg tggaggtctt
     6781 gcgaccttca gtccaccggc ggagctcgcg gcgacatggg agacgacgag gagctcggcg
     6841 atccggacgc tggaccgact cgagaagctg ctcggcctgg tccgcttctg gacgctcgac
     6901 ctgccgaccc agataaccgc gtggctcgcc catatccgcg agatggggac ggtcgatcaa
     6961 ccgacggccg tgtacctcaa ggcgctgcgc gagggcggca agatcggtcc gaacaagcgg
     7021 cccgcctatc agctgcaggc gctggcgcag accgacgact tcaccaggcc cgacggcggc
     7081 gcgatcttcc tgcgggatga gtgtaccgga cgaaaaccca acaagaatgg caaggatgcg
     7141 acgaccatgc cggagatcgt ggccttgcaa ttcttcggca acgagagcgg cctcaagttc
     7201 acgcatggac tggcgccgtt caacacgcgc acggaggaca aggcgaagcg tctcccattg
     7261 ctgggtatcg acaccaacgc gcaccgcaag ggcagcttcg atgctgtctt tcatgccggc
     7321 ggtgaatggt actggagccg cgggtggggc gtcggtcagg cgacggagag ggacggcaag
     7381 ctcgacggtg tcgcgctacg ccgcggcctg ccggtcatgc ccgccgacgc ggacagcgtg
     7441 cagcatccga agccgtatat tgaccgcgag gagagcgtca aggacgcgat ggcgcgcaag
     7501 atcctcgcca agtacaacgc ccgcaccgac aggcgcgact gcaccttcgg cgagaccggt
     7561 gacggaaaat attacgactg tcatcgttgc ctcaagcgct tcgtcgacga cgggctcgtc
     7621 ggttccggcg aatatggcag gggtggggtg ttcgtcccga aggccagggg ctcgatcggg
     7681 tcggtcaaga aggcgtcgag cttcttcgtc gatctcgagc gctacacgcc gttcgcgcgc
     7741 ggcgatgcgg gggtcgagaa tcccctggcg gccggccaat tcgagaatta tttcgccgcg
     7801 agccttccgg aggcggaccc caacgtcgcg gccgtcctgc ggagcctgaa gggcaagcag
     7861 accctcgccg gggcggtcaa gagcacggcc aaggcggccg gtgtgccagc aaaggagctg
     7921 atggccgcgg tgcaggcgca tgtcgacgtc cgctcgcagt ttccttgttc ctggttacag
     7981 gtgaggatcc agtacgccgg cagcggcgag caggcgttcg gctcgctcat cgatcttttc
     8041 aaggtggtcg gggcgctcga caccaagaac gagacgatga taaagcacat caaggaagct
     8101 tctgagctgc ggaggaaagc ttgacgcgca tgcacatcaa cctcacgccc ggtatcatcg
     8161 tcgtgctgac cgcctgcggc ccggggcagc agtcgcagga gggtccgcgg cccgtcagcg
     8221 accccacgcc tgtcgtccaa ccagcgagcc ccgagccgtc cgccgcgccg aaatgtgccg
     8281 atgagccggg tatggtgtac atcgcgggcg gcgaacatct ggccacccat gataacaagc
     8341 gatattccgt ctcgccgttc tggatcgacc gcaccgaggt gac
//
LOCUS       DASWNJ010001699         5826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_452293, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001699 DASWNJ010000000
VERSION     DASWNJ010001699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5826
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452293"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VGB85_28215"
     CDS             complement(<1..648)
                     /locus_tag="VGB85_28215"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEY0138016.1"
                     /translation="MTQAPSSRPPLGTPLSLEDAAAYPLPGLVHPQSVAFTPDDGALT
                     YLRSPERSLTRQLYRLDLTTGEETPLVMPPGGGVTEENLSLEEKLRRERQRVRELGIT
                     TYAWAKEGERLLVPLTGDIHVQDGERGPLRLVVDAAGKPALDPQLSRDGQKLAYVQDD
                     ELYVVDAAGGTPAQRTRGARGTGKTHGLAEYIAQEEMGRSHGFWWSWDGAHLAFTE"
     gene            908..1366
                     /locus_tag="VGB85_28220"
     CDS             908..1366
                     /locus_tag="VGB85_28220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138017.1"
                     /translation="MARLAEILTQPEIRPQVVQACAVLIDSEVQSKSGLSGLAVKAGY
                     KLVKAIKPTMVTDVVDKLLPEFAEAMQPMFAEVEQQSATSGRPRGELFSTHMQADPNR
                     VAAALLTVTDRRAEKASGALQKTYNRLRGTAEEHVQAAIPGLVRTLTTFV"
     gene            1518..3005
                     /locus_tag="VGB85_28225"
     CDS             1518..3005
                     /locus_tag="VGB85_28225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEY0138018.1"
                     /translation="MSMRSVAKTLILSCSLVHVVQVAGCGDDGGTGGSAETSITSATI
                     TNPTTVGSTSDGTTTQGSATESPTEGGGSATESAGGTSTGQLTGVTESSTTEPATATE
                     TATATEPGMTTTTDASGTTTTTGDESGTTTDASGTTTTDGTTTTDGGTTTGGESTTTT
                     MGGDETTEDVPCTMQAATLKPVIPNMMLVLDKSGSMLTKWDHDANPGTPTITRWNSLY
                     QTVDLVLTKFNDKVNFGANLFPNKSAQQVYTDKACLVNANVEIPIKPKNKTAILNGIP
                     AADNMTIAGGTPANAGMAAAIKHLKTFPADVPRAILLVTDGAANCTPGLQPPPLFEKY
                     DDGVHTIVGDAYTVDGIPTYVVGINTLNQTTPPTQDGNPDGINPFLKLNDLAVKGGTP
                     KNDPNEKFYNADNQIELGAALDAIIADALSCVIPLDSEPGKPELTKVKINGADIPHVD
                     NCANENGWVYTNPNGPYDAIELCGTACGGLKQFGKADVNFFCVPG"
     gene            3276..5402
                     /locus_tag="VGB85_28230"
     CDS             3276..5402
                     /locus_tag="VGB85_28230"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HEY0138019.1"
                     /translation="MAALVSPLLFACGDSGVTTDSPTNPSTPTNPSGVDTLTGSMTTS
                     GQPATEPGTPTTGDEGGNSQGTDPGTTTNSPSDPSTTVDTTTTMSSDPSTSSATDGTT
                     STTDASTTDVSGTDGSSSGGPPPPMCTEAECPMGQFCNMQSGLCEPGCNDDTDCNDAT
                     LCDVGEKVCKGCLIDGNCGLGTVCEGGTCVAGCNDMQPCQDGLACCGGQCFDVASDPL
                     HCGGCTACPLPDNAAASCVGGTCGLGACQNDFNNCDKDPGNGCEVDGSCECVPGEKVA
                     CYTGPEETKNIGVCKGGLQTCNAQGTGYGSCTGEVLPGALDLCANSLDDDCDGSVDED
                     PDEDGDGWTVCGGDCCDAIGPNCLNPLLVNPGAFEQGGNMVDDDCDGMKDNAVPVCDA
                     GLASDSNVATDYAKAIDLCKFTTENPPQKMKTWGVISANLFRASGADAPDPSAKSIRT
                     GFGTNVKPQQNSSLAVFSTGFAADKNDTKPAFAPFEPGINLNSDSTPPADWLAANGNK
                     FPNAPGCPAALSNAANDSIMLKVRVRVPTNAKSFSVQMYFYSAEWPEWTCTPYNDMFV
                     TLLNSQGAGNPADKNIAIYTTPNNLKYPVGVNLVKAASGLFTQCKNGQYGCSGNQVAN
                     YNGCTSVAGLAGTGFDDIGTSCAGNNTVGGGTGWLKMAGNVKGGETMEIRFVIWDTSD
                     SALDSLVLLDDWTWSVQASQPGVQPN"
     gene            complement(5485..>5826)
                     /locus_tag="VGB85_28235"
     CDS             complement(5485..>5826)
                     /locus_tag="VGB85_28235"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEY0138020.1"
                     /translation="KDGEWGLFIHGVDGLACTIAGLKLSAAQPFPTGTWTHVGCVYDG
                     QELRVYIDGQMVANTPNNSSIADNNTTLLFGCNSPGCDERMVGGRLDAVRIWTAARTG
                     AEICADADACP"
BASE COUNT         1032 a         2020 c         2007 g          767 t
ORIGIN      
        1 ctcggtgaag gccaggtgcg cgccgtccca cgaccaccag aagccgtggc tgcggcccat
       61 ctcctcctgg gcgatgtact cggcgaggcc gtgggtcttg ccggtcccgc gcgcgccgcg
      121 ggtccgctgc gcgggtgtgc caccggcggc gtcgacgacg tacagctcgt cgtcctggac
      181 gtaggcgagc ttttgtccgt cgcggctgag ctgggggtcg agcgcgggct tgccggcggc
      241 gtcgacgacg aggcgcaggg gtccgcgctc gccgtcctgt acgtggatgt cgccggtcag
      301 cggcacgagc aggcgctcac cctccttggc ccacgcatag gtcgtgatgc cgagctcgcg
      361 cacgcgctgg cgctcgcggc ggagcttctc ctcgagcgag aggttctcct cggtgacacc
      421 gccgccgggt ggcatcacca gcggcgtctc ctcgccggtg gtcaggtcga ggcgatagag
      481 ctggcgggtc aggctgcgct cggggctgcg aagataggtc agcgcgccgt cgtcgggcgt
      541 gaacgcgacg ctctgcgggt ggacgaggcc gggcaggggg tacgcggcgg cgtcctccag
      601 actcaggggt gtgccgaggg gcgggcggga gctgggcgct tgcgtcattg cgggtgcgtc
      661 gggggagcct gatgaagcag atgaagcgga tgaagtagct cgactcgcga cagtgacagc
      721 ggagtcgggg gcgggcgaag gcgtgcgcgg cgagcaggcg gcgagcgcgg cgcaaaggag
      781 cgggacgatc gcggcggcat gcatcgcgcg gcgaggatcg caaccttcgc ggcgcctgtc
      841 cacgccgccg tctccagcgg gatcggcgct tgcctcggcg cgggacttca gccatatagg
      901 cgcacccatg gcccgcctcg ccgagatcct gacccagccc gagatccgtc cccaggtcgt
      961 ccaggcctgc gccgtcctca tcgacagcga agtgcagagc aagagcggcc tgtccgggct
     1021 cgcggtcaag gccggctaca agctcgtcaa ggcgatcaag ccgacgatgg tcaccgatgt
     1081 cgtcgacaag ctgctgcccg agttcgccga ggcgatgcag ccgatgttcg ccgaggtcga
     1141 gcagcagtcc gcgacgagcg gacgcccgcg cggcgagctg tttagcacgc acatgcaggc
     1201 cgacccgaat cgggtcgcgg ccgcgctgtt gaccgtcacc gaccgccgcg ccgagaaggc
     1261 cagcggcgcg ctgcaaaaga cctacaaccg cctgcgcggc accgccgagg agcacgtaca
     1321 ggcggcgatc ccgggcctgg tgcgcacgct gacgaccttc gtgtgatcgc gtgcgggcgc
     1381 aagatcgccc gacacacgat acggcgaggc gcccgccgtg acgccgcgcc acgtgtccga
     1441 cgcggcgaaa aaatatcggc gacgctcgag ggcgtggccg agaatttctg atagtgtcga
     1501 ggcttgcgtt gggggtgatg tcgatgcgaa gcgtggccaa gactttaatt ctttcttgtt
     1561 cgcttgttca tgtggttcag gtcgcgggct gtggcgatga tggcggcacc ggcggaagcg
     1621 ccgagacgtc gatcacctcg gcgacgatca ccaatccgac caccgtgggc tcgacctcgg
     1681 atggcacgac gacccagggt tcggcgaccg agagcccgac cgagggcggc ggctccgcga
     1741 cggagtctgc cggtggcacg agcaccggtc agctcaccgg cgtcaccgag tcgagcacga
     1801 ccgagccggc gacggcgacc gagacggcga cggcgaccga gccgggcatg acgacgacga
     1861 ccgacgcgag cggcacgacg acgacgaccg gcgacgagtc cggcacgacg accgacgcga
     1921 gcggcacgac gacgaccgac ggcacgacga ccaccgacgg tggcacgacg accggcggcg
     1981 agtcgacgac gacgaccatg ggtggcgacg agacgaccga ggacgtgccg tgcacgatgc
     2041 aggcggcgac cctcaagccg gtgatcccga acatgatgct cgtgctcgac aagtcgggct
     2101 cgatgttgac caagtgggac cacgacgcca accccggcac gccgacgatc acgcgctgga
     2161 acagcctgta ccagacggtc gacctcgtgc tgaccaagtt caacgacaag gtcaacttcg
     2221 gcgccaacct gttcccgaac aagtcggcgc agcaggtcta caccgacaag gcgtgcctgg
     2281 tgaacgccaa cgtcgagatc ccgatcaagc cgaagaacaa gacggcgatc ctcaacggca
     2341 tcccggcggc ggacaacatg acgatcgccg gcggcacccc ggccaacgcg ggcatggccg
     2401 cggcgatcaa gcacctcaag accttcccgg ccgacgtccc gcgcgcgatc ctgctggtca
     2461 ccgacggcgc ggccaactgc acccccgggc tccagccgcc gccgctgttc gagaagtacg
     2521 acgacggggt gcacacgatc gtcggcgacg cgtacacggt cgatgggatc ccgacctacg
     2581 tcgtcggcat caatacgctc aaccagacga ccccccccac ccaggacggc aacccggacg
     2641 ggatcaaccc gttcctcaag ctcaacgacc tggcggtcaa gggcggcacg cccaagaacg
     2701 acccgaacga gaagttctac aacgccgaca accagatcga gctgggcgcc gcgctcgacg
     2761 cgatcatcgc cgacgcgctc agctgcgtga tcccgctcga cagcgagccg ggcaagccgg
     2821 agctgaccaa ggtcaagatc aacggcgccg acatcccgca cgtcgacaac tgcgccaacg
     2881 agaacggctg ggtctacacc aaccccaacg ggccgtacga cgcgatcgag ctgtgcggca
     2941 ccgcctgcgg cggcctcaag cagttcggca aggccgacgt gaacttcttc tgcgtgcccg
     3001 gctgagccgg ccccacggcc ccatggcccc acgcccccgc atggggccgc gctcggggac
     3061 gacgagcccc gaacctgacg agacgaccga ggccgcgagc acaagctcgc ggcctcgcgc
     3121 cgtcggtgtg gtcgacgtct cgacggcgtg gtcgcatcgc acagcttcgt gtcctggcgg
     3181 ggccagcggc gggcttctgc caccgcgggc cggccttgtc gccgaccgtc agttgtggct
     3241 acgatgcccg aatgcgatat cacaaggtcg gtagggtggc tgcgctcgtt tctccactgt
     3301 tgttcgcgtg cggcgactcg ggcgtcacca ccgactcgcc gacgaacccg tcgacgccga
     3361 ccaacccgag cggggtcgac acgctgacgg gctcgatgac gacgtcgggc cagcctgcga
     3421 ccgagcccgg cacgccgacg accggcgacg agggcggcaa ctcgcagggc acggacccgg
     3481 gcacgacgac caatagcccg agcgacccgt cgacgacggt cgacacgacg acgacgatgt
     3541 cgtcggaccc ctcgacctcg agcgcgaccg acggcacgac gagcacgacc gacgcctcga
     3601 cgaccgacgt gtcgggcacc gatggttcga gctccggcgg gccgccgccg ccgatgtgca
     3661 ccgaggccga gtgtccgatg ggccagttct gcaacatgca gagcggcctg tgcgagccgg
     3721 gctgcaacga cgacaccgac tgcaacgacg cgacgctctg cgacgtcggc gagaaggtgt
     3781 gcaagggctg cctgatcgac ggcaactgcg ggctcggcac ggtgtgcgag ggcggcacct
     3841 gcgtggccgg ctgcaacgac atgcagccgt gccaggacgg cctggcctgc tgcggcgggc
     3901 agtgcttcga cgtcgccagc gaccccctgc actgcggcgg ctgcacggcg tgcccgctgc
     3961 ccgacaacgc ggcggcgagc tgcgtcggcg gcacgtgcgg gctcggtgcc tgccagaacg
     4021 acttcaacaa ctgcgacaag gaccccggca acggctgcga ggtcgacggg tcgtgcgagt
     4081 gcgtgccggg cgagaaggtc gcctgttaca ccggcccgga ggagaccaag aacatcggcg
     4141 tgtgcaaggg cggcctgcag acctgcaacg cgcagggcac cggctacggc tcgtgcacgg
     4201 gcgaggtcct gcccggcgcg ctcgacctgt gcgccaacag cctcgacgac gactgcgacg
     4261 gctcggtcga cgaggatccg gacgaggacg gcgacggctg gacggtgtgc ggcggcgact
     4321 gctgcgacgc gatcgggccc aactgcctca acccgctgct cgtcaacccc ggcgccttcg
     4381 agcagggcgg caacatggtc gacgacgact gcgacggcat gaaggacaac gcggtccccg
     4441 tgtgcgacgc cggcctggcg agcgactcca acgtcgccac cgactacgcc aaggcgatcg
     4501 acctgtgcaa gttcacgacc gagaacccgc cgcagaagat gaagacgtgg ggcgtgatct
     4561 cggccaacct gttccgcgcc agcggtgccg acgcgccgga ccccagcgca aagtcgatcc
     4621 gcaccggctt cggcaccaac gtcaagccgc agcagaacag cagcctcgcg gtgttctcga
     4681 ccggcttcgc cgccgacaag aacgacacca aaccggcctt cgcccccttc gagcccggca
     4741 tcaacctcaa ctcggacagc accccgccgg cggactggct cgccgccaac ggcaacaagt
     4801 tccccaacgc cccgggctgc cccgcggccc tcagcaacgc cgccaacgac agcatcatgc
     4861 tcaaggtccg cgtgcgcgtg ccgaccaacg ccaagtcgtt tagcgtgcaa atgtacttct
     4921 actcggccga gtggcccgag tggacctgca ccccctacaa cgacatgttc gtgacgctgc
     4981 tcaactcgca gggggccggc aacccggccg acaagaacat cgcgatctac accacgccga
     5041 acaacctcaa gtaccccgtc ggcgtcaacc tggtgaaggc ggcctccggc ctgttcacgc
     5101 agtgcaaaaa cggccagtac ggctgctccg gtaaccaggt ggccaactac aacggctgca
     5161 ccagcgtggc ggggctcgcg ggcaccggct tcgacgacat cggcacgtcg tgcgccggca
     5221 acaacaccgt cggcggcggc accggctggc tcaagatggc aggcaacgtc aagggcggcg
     5281 agacgatgga gatccgcttc gtcatctggg acacgtccga cagcgcgctc gactcgctcg
     5341 tgctgctcga tgactggacc tggtcggtcc aggcctcgca gccgggcgtg cagccgaact
     5401 gatcggctcg tgtagggacg ctcccggggc acgcgcctcg ggggcgtctc gcgttcggtg
     5461 gatagccttg gcggcgtcga gcgctcaggg acaggcatcg gcgtcggcgc agatctcggc
     5521 gccggtcctc gcggcggtcc agatgcggac ggcgtcgagg cgcccaccga ccatacgctc
     5581 gtcgcagccg gggctgttgc agccgaacag cagcgtcgta ttgttgtccg cgatcgagct
     5641 gttgttggga gtattcgcga ccatctgacc gtcgatgtag acgcgcagct cctgaccgtc
     5701 gtatacgcag ccgacgtggg tccatgtgcc ggtcgggaac ggctgcgcgg cggagagctt
     5761 gagccctgcg atcgtgcagg cgaggccatc gaccccgtgg atgaacaggc cccactcgcc
     5821 gtcctt
//
LOCUS       DASWNJ010001700         3148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_170240, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001700 DASWNJ010000000
VERSION     DASWNJ010001700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170240"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VGB85_28240"
     CDS             complement(<1..461)
                     /locus_tag="VGB85_28240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138021.1"
                     /translation="MTGIQSTARLLLALAPLLGLASACDKAEPVDKQPVADSKGDTEG
                     DSESASPTTTEGDDSEGDPETTSTSDPLPEDECGWFLVGMTPDEISAAWTEAQSFGAP
                     EQPYWCEGEHSMPCTTAEGMAGTSLCVWSWGEQVWTTCAVEHACTPDTTFE"
     gene            complement(605..994)
                     /locus_tag="VGB85_28245"
     CDS             complement(605..994)
                     /locus_tag="VGB85_28245"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEY0138022.1"
                     /translation="MQSILRTTTLALACLLPLASACSQPAEGKLDEDLIRRVVEAHMA
                     ETRACYEVLLVSDADASGVLAVDFTIDESGEVRDPVIHGDDLTDATLHDCMRDTMLAW
                     KFPRPEGGHVTVTYPFLFMPNDPRFPS"
     gene            1397..>3148
                     /locus_tag="VGB85_28250"
     CDS             1397..>3148
                     /locus_tag="VGB85_28250"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEY0138023.1"
                     /translation="MHRGRQRPLQQHVWRAFLRVALLPLLLVEVALIAVYLMANTAAT
                     GENLAAVRALADDELARIAAQEAENIDAELEGVARTTTLFARQSAEALARPYTPPPEQ
                     LAAYGTSADGIYYTLRDPGGSALYYSNVTQLGPEQRDKALRTAQLDPLMRDILATHPI
                     VVQLYLNTFDSMNRILPYFDVLSQYAPKMDIPTYNFYYEADATHNPGRKTVWTDAYVD
                     PAGKGWMVSCIAPVYRGDFLEGVVGLDLTTDKVVREVLDLRLPWGGYGVLLGKDGTVL
                     ALPPAGEADWGLHELTEHSYTTAIMQDTFKPEAFNVFKRTDLASLAAVVRDAPRGVAA
                     VSLGGPRVAAWATVSQTGWTLVLLAPEDGIYAQASSLGARLFRIGALMVLGLVLFYAV
                     FLVFVARRARAVAAEITTSLLEVDTLVRKIGAGEYTQEPPPLAISELAASAAGVAAMG
                     EQLRATNQSLVAARREALEARDSALQASRLKSEFLATMSHELRTPLNIILGMNDLLRE
                     DLQDEVLRRHAAAVDTAGRDLLRIVEDVLDLSRIEAGKVDLERLPFSLAATVESVTEL
                     MIQQARSRGLHLVVHVDP"
BASE COUNT          465 a         1087 c         1134 g          462 t
ORIGIN      
        1 tcctcgaacg tggtgtcggg ggtgcaggcg tgctcgaccg cgcaggtcgt ccacacctgc
       61 tcgccccacg accacacgca gagcgacgtg ccggccatac cctcggcggt ggtgcagggc
      121 atcgagtgct cgccttcgca ccaatagggc tgctccggag cgccaaacga ctgcgcttcg
      181 gtccacgcgg cgctgatctc gtcgggggtc atgccgacga ggaaccaccc acattcatcc
      241 tcggggagcg ggtcgctcgt gctggtggtc tccgggtcgc cctcggagtc gtcgccctcg
      301 gtggtcgtgg gcgacgccga ctcggagtcg ccctcggtgt cacccttgga gtcggcgacg
      361 ggctgcttgt cgaccggctc ggccttgtca caggcagagg cgaggccgag gagcggcgcg
      421 agggcgagga gcaggcgggc ggtcgactgg atgccggtca tgtccggcac tgatgcacag
      481 gtgatgccag atcgatctcc ggggcccgtg gcggcccgaa ggagcccgtt tccccgtcgg
      541 tccatgacac gcgtgtcagg tcctgccgtc ggtgtcggcg ttggccgcga cctcgacgcg
      601 ccggtcacga ggggaagcgc gggtcgttgg gcatgaacag gaacggatag gtgacggtga
      661 cgtggccgcc ctcggggcgg ggaaacttcc acgcgagcat cgtgtcgcgc atgcagtcgt
      721 gcagggtcgc gtcggtgagg tcgtcgccgt ggatgacggg gtcgcggacc tcgccggact
      781 cgtcgatcgt gaagtcgacc gccagcacgc cgctcgcgtc ggcatcggag acgaggagca
      841 cctcgtaaca cgcgcgggtc tcggccatgt gggcctcgac gacccgacgg atgaggtcct
      901 cgtcgagctt gccctcggcc ggctgcgagc atgctgacgc gagcgggagc agacacgcga
      961 gcgcgagggt ggtggtgcgg aggatcgatt gcatacccgg tctcacggcg cccacggccg
     1021 ggagttcccg ccaagcgaaa aaactttcga cccggggcgg cggcgcccgg gtgagcgcag
     1081 cgaggatcgt ggcccggggg cgcgatggac gaagcttgct cgaactcgcg ccgcccgcgg
     1141 ggtgggtcga ggggcacgcc gcgtggggcc gggtgatcgg ctcgcgatct tccgtcaccg
     1201 cgaggaggtg ctcgcgagac ccgacgttcg atcgccatga gacgcggcgt ggacaccctc
     1261 gcctcgccgt cggtacacca cgacagcaag cacgcggggc ccacaaaagc cggtcgtgag
     1321 gcacgagcac cgcacctgcg agcgcgcgat gctggcgcct gcgcggggca tcgagcgtgt
     1381 tatacacggg ccgaaggtgc atcgagggcg acaaaggccg ctgcaacagc acgtgtggcg
     1441 agcgttcctg cgggtcgcgc tgctgcctct gctgctcgtc gaggtcgcgc tgatcgccgt
     1501 gtacctgatg gcgaacacgg cggcgaccgg cgagaacctc gcggccgtgc gcgcgctcgc
     1561 cgacgacgag ctggcccgca tcgccgcgca ggaggccgag aacatcgacg ccgagctcga
     1621 gggcgtcgcc cgcacgacca cgctgttcgc ccggcagtcg gccgaggcgc tggcccggcc
     1681 atacacgccg ccgccggagc agctcgcggc gtacgggacc tcggccgacg gcatttatta
     1741 cacgttacgc gaccccggcg gcagcgcgct ctattacagc aacgtcacgc aactgggccc
     1801 ggagcagcgc gacaaggcgc tgcgcaccgc gcagctcgat ccgctgatgc gcgacatcct
     1861 cgcgacccac ccgatcgtcg tgcagctgta tctcaacacc ttcgactcca tgaatcggat
     1921 cctgccgtac ttcgacgtgc tctcgcagta cgcgccgaag atggacatcc cgacgtacaa
     1981 cttttattac gaggcggacg cgacccacaa ccccgggcgc aagacggtgt ggaccgacgc
     2041 ctacgtcgat ccggccggca aggggtggat ggtctcctgc atcgcgccgg tgtaccgcgg
     2101 cgacttcctc gagggcgtcg tcgggctcga cctgaccacc gacaaggtcg tgcgcgaggt
     2161 cctcgatctg cgcctgccgt ggggcggtta tggcgtgctg ctcggcaagg acggcacggt
     2221 gctggccctg ccgcccgcag gcgaggccga ctggggcctg cacgagctga ccgagcactc
     2281 ctacacgacc gcgatcatgc aggacacctt caagccggag gcgttcaacg tgttcaagcg
     2341 gacggacctc gcgtcgctcg cggcggtcgt gcgcgacgcg ccgcggggcg tcgccgcggt
     2401 ctcgctcggc gggccgcggg tcgccgcgtg ggccacggtg tcacagaccg gctggacgct
     2461 ggtcctgctc gcgcccgagg acggcatcta cgcgcaggcc agctcgctcg gcgcgcggct
     2521 gtttcgcatc ggcgccttga tggtgctcgg cctcgtgctg ttctacgcgg tgttcctggt
     2581 gttcgtcgcc cgccgcgcgc gggccgtcgc cgccgagatc acgacctcgc tgctcgaggt
     2641 cgacaccctc gtgcgaaaga tcggcgccgg cgagtacacc caggagccgc cgccgctggc
     2701 gatctccgag ctcgccgcga gcgcggccgg ggtcgcggcg atgggcgagc agctgcgcgc
     2761 gaccaaccag agcctcgtcg ccgcccgacg cgaggcgctc gaggcccgtg acagcgccct
     2821 gcaggcctcg cggctcaagt cggaattcct ggcgacgatg agccacgagc tgcgcacgcc
     2881 gctcaacatc atcctcggca tgaacgacct gctgcgcgag gacctgcagg acgaggtcct
     2941 gcgccgccac gccgccgcgg tcgacaccgc cgggcgcgac ctgctgcgga tcgtcgagga
     3001 cgtgctcgac ctgtcgcgca tcgaggccgg caaggtcgac ctcgagcgcc tgcccttctc
     3061 gctggcggcc accgtcgagt cggtgacgga gctgatgatc cagcaggcgc gctcgcgcgg
     3121 gctgcacctc gtggtgcacg tcgacccg
//
LOCUS       DASWNJ010001701         7064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_278455, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001701 DASWNJ010000000
VERSION     DASWNJ010001701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7064
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278455"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VGB85_28255"
     CDS             <1..294
                     /locus_tag="VGB85_28255"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0138024.1"
                     /translation="VTRTRARFAILDLTGVDVLDTATANHLLGMIRALRLLGAEGVLT
                     GIHPHVAQTVVTLGVDLSTLVVRANLRDALKYALAVLARTDRNRRTVATDEQG"
     gene            303..1460
                     /locus_tag="VGB85_28260"
     CDS             303..1460
                     /locus_tag="VGB85_28260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0138025.1"
                     /translation="MSGSHPEMVNTQWMQGAPLAAARVDAAGNVLAWNPAAERMFGVR
                     RDDAVGRPIAAVVPVPGEDGAWQALLASGEPARREWELRRDGQQIVKCAWMVQPLRDG
                     GLVCYGEEIADATHEARQQKIRDLLLRAMLDTLDIVAWVTDERGIGIMVDGKGLARTG
                     VTAEQLKGLNLVEIYGAKYPQIHDGIKGTPVHFFAQEGGRHFEDWAVPMRNEQGQQIA
                     LVGFALDITEAKLREEELQNKIDLIQRQQDAMRDMSTPIIEVWDQVICLPLVGLVDTA
                     RTAEIMDNLLQAVTRTRARFAILDLTGVDVLDTATANHLLGMTRALRLLGAEGVLTGI
                     HPHVAQTVVSLGIDLSTLIVRANLRDALKYALGVLARKDRNQRTTATDEQG"
     gene            1463..2581
                     /locus_tag="VGB85_28265"
     CDS             1463..2581
                     /locus_tag="VGB85_28265"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0138026.1"
                     /translation="MEHTSEPTASSELIQGAPFVVVQVDAAGKVLTWNTAAERMFGVR
                     GEDAIGRPIAAVAPVPGEDGAWQALLAAGKPARREWEVRKDGPRVIKCAWTVQPLQAG
                     GLACYGQELDDPTHEARQQKIRDLLLRAMLDTLDIVAWVTDKHGNATLVDGKGLARTG
                     LTPEQMLGRNIIEIYGARYPVMHGVLEGQPIHLSGQEGPAHFETWAVPMRDEHGQQVA
                     VVAFSLDITEAKVREQELQNKIDLIQRQQQAMREMSTPIIEVWDHVICLPLVGLVDTA
                     RTADIMESLLQAVTRTRARYAILDLTGVDVLDTATANHLLGMTRALRLLGAEGVLTGM
                     HPNVARTVVTIGVDLSTMVVRATLRDALKFVLGALERR"
     gene            2619..3728
                     /locus_tag="VGB85_28270"
     CDS             2619..3728
                     /locus_tag="VGB85_28270"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0138027.1"
                     /translation="MDTDVIEAETIAAAPFAVAEVDAGGKLLAWNLAAERMFGVAQAD
                     AIGRPIGAVAPVPGGHAAWQAVLAAGKLAKITVDGSQTTQRTWTVQPRSDGGALCYGH
                     EVAGATADAIQQKARDLLLRGMLDTLDIFAWVTDERGIGVLADGKGLARTGVTAERLR
                     GQNMVDMYGTKYPQIHDGLKGRPVHFIAEEGAGVFENWGVPVHDEHGRQIALVGFAID
                     ISKARSREDDLADQLQVIESQERAMRDMSTPVIQVWDHVLCMPLVGLVDTARTAEIME
                     SLLQAVARTRARYAILDLSGVDVLDTATANHLIVMTRALRLLGAECVLTGMQPNIART
                     VVTIGVDLSTMVVRATMRDALKFVLGALEHPDERL"
     gene            3765..5234
                     /locus_tag="VGB85_28275"
     CDS             3765..5234
                     /locus_tag="VGB85_28275"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0138028.1"
                     /translation="MTARRLRTPLFLALGLLALTACKKDAIEIKPPDPVVAVPRPEDP
                     GVVSLASALPQRLVAADKTSEVRVRLRIDASKLAHSKRPPINVVLVMDSSGSMEGKAI
                     EDARAAAVALVDGLAEGDRLAVVSFDSLSHVLVPSTGIDATSQSKIREQIATMTARGT
                     TDLAAGLAAGLAEASRGYLNEGVNRLVLLSDGVPNDESPVSALAQQAASMRVSVTALG
                     LGLEYNETLLTSLAQTTGGKFHFIEDSTQVATVFRDEVLRLERVVAQNVQLTLLAGPG
                     VTVEGVLGHGTTPAGSQRTMSLQLGDLSEGSHRDLIVRLRVGPHRDGASLELMDALLN
                     FQDAVAGAGALERRLYVAARTSADAAKIADSRDPELERQIARALADSATVDAIALARS
                     GQLAAAVTLIDHVEPETRTAAKLLGDHDLKLQADGLVKLRKALPTLLPQSQPLAIAGP
                     VAPNSPQPTATMAMPIAADDSPATVKRSHGKAMQRLQGE"
     gene            complement(5267..5419)
                     /locus_tag="VGB85_28280"
     CDS             complement(5267..5419)
                     /locus_tag="VGB85_28280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138029.1"
                     /translation="MLTIILIVLLVLALGGGGVGHSRYGFAGWSPAFLILLVLLVLWY
                     TGHLHG"
     gene            complement(5606..6871)
                     /locus_tag="VGB85_28285"
     CDS             complement(5606..6871)
                     /locus_tag="VGB85_28285"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0138030.1"
                     /translation="MERIDGPTGQKGASLADAFNPFGGAQLADPYPVYARLRAEAPVF
                     YSPLLRMWYVTRYDDIVAVLKDPARFSAVAAINTPLDYTEETRRAAQALYITERTLAD
                     NDPPAHTSIRRLVSKAFTVRLPAMEARVRAIAERLLDGFVADGRAEFLSQFAYPMPML
                     VILAMLGAPEDDMPLIKRWGADWVTHISIQQTPEQQAATIGRLADSQRYWSELIAARR
                     ADPRDDLLSDLIAASKSEATELSNLQIVNLCSTLVLAGHETTANVLGHCLHRLLRDRE
                     QWRLVLDDPANIPRAFEETLRADTSVQALMRTTREPVELGGVAIPAGAQVAMLFASGS
                     HDAAYFVDAARFDLRRESPRTHLAFGHGVHFCLGAPLARIEGRVAIELLARRLPGLRL
                     AEQEITYVPNPMVRALTALQLEWDAPSRG"
BASE COUNT         1141 a         2613 c         2346 g          964 t
ORIGIN      
        1 gtcacccgca cccgcgcccg cttcgccatc ctcgacctca ccggcgtcga cgtcctcgac
       61 accgcgaccg ccaatcatct cctcggcatg atccgcgcgt tgcgcctgct cggcgccgag
      121 ggcgtgctca ccggcatcca ccctcacgtc gcccagaccg tcgtcaccct cggcgtcgac
      181 ctctcgaccc tcgtcgtccg cgccaacctg cgcgacgccc tcaagtatgc cctcgccgtc
      241 ctcgcgcgga cggaccgtaa tcgacgaacc gtcgccaccg acgagcaagg ctgatgatga
      301 acatgagcgg atctcacccc gagatggtca acacccaatg gatgcagggc gcccctctcg
      361 cggccgcgcg ggtcgacgcc gcgggcaatg tgctcgcgtg gaaccccgcc gccgagcgca
      421 tgttcggcgt ccgtcgcgat gatgcggtcg gtcgaccgat cgccgcggtc gtgccggtcc
      481 cgggggagga cggcgcctgg caggccctgc tcgccagcgg cgagccggcc cgccgcgagt
      541 gggagctgcg ccgcgacggc cagcagatcg tcaagtgcgc gtggatggtc cagccgctgc
      601 gcgacggcgg cctcgtctgc tacggcgagg agatcgccga cgcgacgcac gaggcccggc
      661 agcagaagat ccgcgacctg ctgctgcgcg cgatgctcga caccctcgac atcgtcgcct
      721 gggtcaccga cgagcgcggc atcgggatca tggtcgacgg aaaggggctg gcgcgcaccg
      781 gtgtcactgc cgagcagcta aagggcctca acctggtcga gatctacggc gcgaagtacc
      841 cgcagatcca cgacggcatc aagggcacgc ccgtccattt cttcgcccag gagggcggcc
      901 gccacttcga ggactgggca gtcccgatgc ggaacgagca aggccagcaa attgccctcg
      961 tcggcttcgc cctcgacatc acggaggcca agctccgcga ggaggagctg cagaacaaga
     1021 tcgacctgat ccagcgccag caggacgcga tgcgcgacat gtcgaccccg atcatcgagg
     1081 tctgggacca ggtcatctgc ctgccgctcg tcggcctcgt cgacaccgcc cgcaccgccg
     1141 agatcatgga caacctgctg caggcggtca cccgcacccg cgcccgcttc gccatcctcg
     1201 acctcaccgg cgtcgacgtc ctcgacaccg cgaccgccaa tcatctcctc ggcatgaccc
     1261 gcgcgttgcg cctgctcggc gccgagggcg tgctcaccgg cattcaccct cacgtcgccc
     1321 agaccgtcgt ctcgctcgga atcgacctgt cgaccttgat cgtccgcgcc aacctgcgcg
     1381 acgccctcaa gtatgctctt ggcgtcctcg cgcggaagga ccgtaatcag cggaccaccg
     1441 cgaccgacga gcaaggctga tcatggaaca cacgagcgaa ccgacagcga gctccgagct
     1501 cattcagggt gcccccttcg tggtcgtcca ggtcgacgcc gcgggcaagg tgctcacatg
     1561 gaataccgcc gccgagcgca tgttcggcgt ccgcggcgag gatgcgatcg gccggccgat
     1621 cgccgcggtc gcgccagtcc cgggggagga cggcgcctgg caggccctgc tcgccgccgg
     1681 taaaccggcg cgccgcgagt gggaggtacg caaggacggc ccgcgggtca tcaagtgcgc
     1741 ctggacggtc cagccgctgc aagccggcgg gctcgcgtgt tacggccagg agctcgacga
     1801 cccgacgcac gaggcccggc agcagaagat ccgcgacctc ctgctgcgtg cgatgctcga
     1861 caccctcgat atcgtcgcct gggtgaccga caagcacggc aatgcgaccc tggtcgacgg
     1921 caagggcctg gcgcgcaccg ggctcacgcc cgagcagatg ctcggccgca acatcatcga
     1981 gatctacggc gcgcggtacc cggtgatgca cggggtcctc gagggccagc ccattcacct
     2041 gagcggccag gagggccccg cccacttcga gacctgggcg gtcccgatgc gcgacgagca
     2101 cggccagcag gtcgcggtcg tcgccttcag cctcgatatc accgaggcca aggtccgcga
     2161 gcaggagctg cagaacaaga tcgacctgat ccagcgtcag cagcaggcga tgcgcgagat
     2221 gtcgaccccg atcatcgagg tctgggatca cgtgatctgc ctgccgctcg tcggcctcgt
     2281 cgacacggcg cgcaccgccg acatcatgga gagcctgctg caggcggtca cccgcacccg
     2341 cgcccgctac gcgatcctcg acctcaccgg cgtcgacgtc ctcgacaccg cgaccgccaa
     2401 ccacctcctc ggcatgaccc gcgcgctgcg cctgctcggc gccgagggcg tgctcaccgg
     2461 catgcacccg aacgtcgccc gcacggtcgt cacgatcggc gtcgacctct cgaccatggt
     2521 cgtccgcgcc accctgcgcg acgccctcaa gttcgtgctc ggcgccctcg agcgccgcta
     2581 aaaggcgacc gcgaagcacc accagcgagg acatggcgat ggacaccgac gtgattgaag
     2641 ccgagacgat cgccgccgcc cccttcgccg tcgccgaggt cgacgccggc ggcaagctgc
     2701 tcgcatggaa cctcgcggcg gagcgcatgt tcggcgtcgc ccaggccgac gcgatcggcc
     2761 gcccgatcgg cgccgtcgcg ccggtcccgg gaggacacgc cgcctggcag gccgtgctcg
     2821 ccgcgggcaa gctcgcgaag atcaccgtcg acggctcgca gacgacgcag cggacgtgga
     2881 ccgtgcagcc gcgcagcgac ggcggagcgc tgtgctacgg ccatgaggtc gccggcgcga
     2941 ccgcggacgc gatccagcaa aaggcccgcg atctgctgct gcgcgggatg ctcgacaccc
     3001 tcgacatctt cgcgtgggtc accgacgagc gcggcatcgg ggtcctcgcc gacggcaagg
     3061 ggctggcgcg caccggcgtc acggccgagc ggctgcgcgg ccaaaacatg gtcgatatgt
     3121 acgggacgaa gtacccgcag atccacgacg gcctcaaggg tcgaccggtt cacttcatcg
     3181 ccgaggaggg cgcgggggtc ttcgagaact ggggggtccc cgtgcacgac gagcacggcc
     3241 ggcagatcgc gctcgtcggc ttcgccatcg acatcagcaa ggcccgcagt cgcgaggacg
     3301 atctcgcgga ccagctccag gtgatcgaga gccaggagcg ggcgatgcgt gacatgtcga
     3361 ctccggtcat tcaggtctgg gaccacgtgc tgtgcatgcc gctcgtcggc ctcgtcgaca
     3421 cggcgcgcac cgccgagatc atggagagcc tgctgcaggc ggtcgcgcgc acccgcgccc
     3481 gctacgcgat cctcgacctc tccggcgtcg atgtcctcga caccgcgacc gccaaccatc
     3541 tcatcgtcat gacccgcgcg ctgcgcctgc tcggggccga gtgcgtgctg accggtatgc
     3601 agcccaacat cgcccggacc gtcgtcacga tcggcgtcga cctctcgacc atggtcgtcc
     3661 gcgccaccat gcgcgacgcc ctcaagttcg tgctcggcgc cctcgagcac cccgacgaac
     3721 gcctgtaagc ggccccgcgg ggccggcgtt tcggggcaca cgccatgact gcccgccgcc
     3781 tccgcacccc gctgttcctc gctctcggcc tgctcgccct caccgcctgt aaaaaggacg
     3841 ccatcgagat caagccgccc gaccccgtgg tcgccgtccc gcgcccggag gaccctggcg
     3901 tcgtgagcct cgccagcgcg ctgccacagc gcctggtcgc cgccgacaag accagcgagg
     3961 tccgcgtccg cctgcgcatc gacgccagca agctcgcgca cagcaagcgc ccgccgatca
     4021 acgtcgtgct ggtgatggac agctcgggct cgatggaggg caaggcgatc gaggacgccc
     4081 gcgccgccgc ggtcgccttg gtcgatggcc tcgccgaggg tgaccgcctc gcggtcgtca
     4141 gcttcgactc cctctcgcac gtgctcgtgc cctcgaccgg gatcgacgcg acgagccaga
     4201 gcaagatccg cgagcagatc gccacgatga ccgctcgtgg caccaccgac ctcgccgcag
     4261 gcctcgccgc aggcctcgcc gaggcctcgc gcggctacct caacgagggc gtcaatcgcc
     4321 tggtcctgct cagcgacggc gtccccaatg acgagagccc ggtgtccgcg ctcgcccagc
     4381 aggccgcgtc gatgcgcgta tcggtgaccg ccctcggcct cggcctcgaa tacaacgaga
     4441 ccctgctgac cagcctcgcg cagaccaccg gcggcaagtt ccatttcatc gaggactcga
     4501 cgcaggtcgc caccgtgttc cgtgacgagg tcctgcggct cgagcgggtc gtcgcccaga
     4561 acgtgcagct gacgctgctc gccggccctg gcgtcacggt cgagggcgtg ctcggccacg
     4621 gcacgacgcc ggccggcagc cagcgcacga tgtccttgca gctcggcgac ctcagcgagg
     4681 gcagccaccg cgacctgatc gtgcgcctgc gcgtcgggcc gcaccgcgac ggcgcctcgc
     4741 tcgagctgat ggacgcgctg ctcaacttcc aggacgcggt cgccggcgcc ggcgcgctcg
     4801 agcggcgcct ctacgtcgcg gcgcgcacca gcgcggacgc agcgaagatc gccgacagcc
     4861 gcgaccccga gctcgagcgg cagatcgccc gcgcgctcgc cgactctgcc accgtcgacg
     4921 cgatcgcgct ggcgcgcagc ggccagctcg ccgccgctgt cacgttgatc gaccacgtcg
     4981 agcccgagac gcgcaccgcc gccaagctgc tcggcgacca cgacctcaag ctccaggccg
     5041 acggcctcgt caagctgcgc aaagcgctgc cgaccctgct accccagtct cagccgctgg
     5101 cgatcgccgg cccggtcgcg ccgaacagcc cgcaaccgac cgccacgatg gcgatgccga
     5161 tcgctgccga cgacagcccc gcgaccgtca agcgctcgca cggcaaggcg atgcagcgcc
     5221 tgcaaggcga gtgagcggcg ggccagtctt cactggcccg cgcggatcag ccatgaagat
     5281 ggccggtgta ccacagcacc agcaggacca gcaggatcag gaacgcgggc gaccagccgg
     5341 cgaagccgta gcgagagtga ccgacgccgc cgccaccgag ggcgagcacg agcaacacga
     5401 tgaggatgat cgtcaacatg ggatcacctt aagcccgatc gctcgatagg ctcgccggtc
     5461 gtgccggcga atgcatcgat ctcgatctgc gtcggctgtg cgggccgtgc cgcgtttacg
     5521 tcggttccgc gccgaatggt ctgcgcagca gccgcgaggc ccatacggag tttcgtcggt
     5581 tatccgcgac gcacgaggct gccaatcacc cgcgcgacgg cgcgtcccac tcgagctgca
     5641 gagccgtgag cgcgcggacc atcgggttcg ggacgtacgt gatctcctgc tcggcgaggc
     5701 gcagccccgg gagacggcgg gccagcagct cgatcgccac acggccctcg atgcgcgcga
     5761 gcggggcacc gaggcaaaag tgtacgccgt ggccgaacgc gaggtgggtc ctgggcgact
     5821 cgcgccgcag gtcgaagcgg gcggcgtcga cgaagtaggc cgcgtcgtgg ctgccggagg
     5881 cgaacagcat cgcgacctgg gcgcccgccg ggatcgccac gccgccgagc tcgaccggct
     5941 cgcgggtcgt ccgcatcagc gcctgcaccg aggtgtcggc gcgcagcgtc tcctcgaacg
     6001 cgcgcgggat gttggcgggg tcgtcgagga ccaggcgcca ctgctcgcgg tcccgcagca
     6061 ggcggtgcag gcagtggccg agcacgttgg cggtcgtctc gtggccggcg agcacgagcg
     6121 tcgagcagag gttgacgatc tgtaaattgc tcagctcggt cgcctcggac ttcgaggccg
     6181 cgatcaggtc actgagcagg tcgtcgcgcg ggtcggctcg acgggcagcg atcagctcgg
     6241 accagtagcg ctgcgaatct gcgaggcggc cgatcgtggc ggcctgctgc tcgggggtct
     6301 gctgaatgct gatatgcgtg acccagtccg cgccccagcg cttgatcagc ggcatgtcgt
     6361 cctcgggcgc gccgagcatc gccaggataa ccagcatcgg catcgggtac gcgaactgcg
     6421 acaggaactc ggcgcgaccg tcggcgacga agccgtcgag caggcgctcg gcgatcgccc
     6481 gcacacgcgc ctccatcgcc ggcagccgca ccgtgaacgc cttgctgacc aggcgccgga
     6541 tgctcgtgtg cgccggcggg tcgttgtcgg ccagcgtgcg ctccgtgatg tacagcgcct
     6601 gcgcggcgcg ccgcgtctcc tcggtgtagt cgagcggcgt gttgatcgcg gcgaccgccg
     6661 agaagcgcgc gggatccttg agcaccgcga cgatgtcgtc gtagcgggtc acgtaccaca
     6721 ttcgcaacag cgggctgtag aacaccggcg cctcggccct cagccgcgca taaaccgggt
     6781 acgggtccgc cagctgcgcg ccgccgaagg gattgaacgc atcggcaagc gacgcgccct
     6841 tttgccccgt gggaccatcg atccgttcca tatccgttcg ttatactggc gcggccacac
     6901 ggcgcctcgc gaaaatatct cgtcggagct cggacactac gacgatgcgc gtccttgcac
     6961 atccaacgac acgacgggct cgccacgaaa tgcgagggcc ccgggtcctc gcaaccgagg
     7021 agccggggcc tgctcacctt cacgcgatcg tggaggtccg cgct
//
LOCUS       DASWNJ010001702         2491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_135424, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001702 DASWNJ010000000
VERSION     DASWNJ010001702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135424"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VGB85_28290"
     CDS             complement(<1..479)
                     /locus_tag="VGB85_28290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138031.1"
                     /translation="MKYLDPRLPLLVVLVASGCPPGENLTDADTTESSGASEAGTTDK
                     PATTSGGTSGQPETTSTSGDTTGQPDTTSTSGETTGQADTTSASGDTTGQPDTTSTSG
                     DTSGTDSTSTGGSSTSGDTMTGDTTTGDMTTGDPVGGCPGGWTRARTISITNEPEKPL
                     "
     gene            1230..2480
                     /locus_tag="VGB85_28295"
     CDS             1230..2480
                     /locus_tag="VGB85_28295"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEY0138032.1"
                     /translation="MSDEDGVGETSALSGREAAALLGVKRETLYAYASRGLLRSEPGG
                     PGRERLYNREDLERLRARRDARSGHGPVAAGALRWGEPVLESALTAIDPSGPRYRGQP
                     AVELAASASFEAVAELLWSGALPDPAPRWEPAGVGLRVASLTALLPGDTPPLVTLALA
                     VAALGAADPGRYNHAIEAEKTRARGLIFRMAALAGAGIDPAQVRPAQAEPDVAGALLV
                     SLGVQATARARRLMSRALVVCADHELNASAFAVRVAASAGADLYACVSAGLATLSGPR
                     HGGVVDRVEALVTEVGRPERAQEVVQERARRGEEVPGFGHPLYPAGDPRAALLLAAAI
                     EHAPRSPRLRTLEAIVAAMREAGREPPNLDIGLASTGVALGIPLRTSVAMFAVGRAAG
                     WIAHAFEQRAAGFLLRPRARYVGP"
BASE COUNT          344 a          854 c          930 g          363 t
ORIGIN      
        1 agaggcttct ccggctcgtt ggtgatagag atcgtgcgcg cccgggtcca gccccccgga
       61 cagccgccga caggatcgcc cgtggtcata tcgcccgtgg tcgtatcgcc cgtcatcgta
      121 tcgccgctgg tcgaggatcc cccggtcgac gtgctgtctg tgccgctggt gtcgcctgag
      181 gtagaggtgg tatcgggttg accggtcgtg tcgcccgacg ccgacgtggt atcagcctgc
      241 ccggtggtct cgccggaggt ggaggtggta tcgggctggc cggtcgtatc gccggaggtg
      301 gaggtcgttt caggctggcc ggaagtaccg ccgctcgtgg tggcaggctt gtctgtggtg
      361 cctgcctcgc tggcaccgct ggactcggtg gtgtcagcat ccgtcaggtt ctcgccaggc
      421 ggacatccag aagcgacgag gacgacgagg agcggcagac gcgggtcgag gtacttcatg
      481 gcgtgtatcg ccagtggcgc agctttggcg agaaagcgca aggcgaaaga cgatagagcc
      541 agaatccgcg gagacatgtg ggacgccgcg gacaccgagc ggcggcgccc gcaaacgcct
      601 gcgtcgcggc gatggccggg gtgggccaca gcggggcgaa cggctgcgag ccgcccgccg
      661 ggaagttcag ctgcagtcgc gggtcttgcg atgtgcagac cgaatattgc cagcacacgg
      721 tcgggcacgg cgagccagag tcgtggcatt gcctcgcgct cgacggccct gcggacgcca
      781 tgggctgcgc gtgcatcacc gacccgtcgc cgatccggag tggtacccgg gtcagtggtg
      841 caacaacctc aagggcggag ggaccgttca catgcctacc atcgtgacgc aaaggccagg
      901 accgacttca ggcgatctgg aagatgaccc actcggggaa cgaactcaag accacggacc
      961 ttgtgcccgt gggcaaagac cgcgaccagc caagctctgg gccgcggagc agatcgagcg
     1021 cgtcttcccc attctcatat ccaaggctgg catcgacgcc gcgcccgaag tccagaccag
     1081 aaaggaggcc gctgcggctt gccctggtcc gatgtcgacc ttcgcagagg cgaagaaatc
     1141 tgcaggctga ggcctgaggg cgttctgggc gaaacagttg atcaacgttg attcgacaat
     1201 ccatcacgtc gcgcgatatt cttttaagaa tgagcgacga ggacggggtc ggggagacca
     1261 gcgcgctgtc ggggcgcgag gcggcggcgc tgctcggagt caagcgcgag acgctgtacg
     1321 cctacgcgag ccgcgggctc ctgcgcagcg agccgggcgg tccggggcgc gagcggctct
     1381 acaaccgcga ggacctcgag cggctgaggg cgcgacgcga cgcccgttcc ggccacggcc
     1441 cggtcgccgc gggggcgctg cggtggggcg agccggtgct cgagtcggcc ctcaccgcga
     1501 tcgacccgag cgggccgcgc tatcgcgggc aacctgcggt cgagctcgcc gcttcggcga
     1561 gcttcgaggc ggtcgccgag ctgctgtggt ccggcgcgct gccggacccg gcgccgcgct
     1621 gggagcccgc gggggtcggg ctgcgcgtcg cctcgctgac cgcgttgctg ccgggggaca
     1681 ccccgccgct cgtcacgctc gcgctggccg tcgcggccct cggcgccgcc gatcccgggc
     1741 gctacaacca cgcgatcgag gccgagaaga cgcgcgcccg cgggctcatc tttcgcatgg
     1801 ccgcgctcgc cggcgcgggc atcgacccgg cgcaggtccg gcccgcgcag gccgagcccg
     1861 acgtcgcggg tgcgttgctg gtgtcgctcg gcgtgcaggc gaccgcgcgc gcgcggcgat
     1921 tgatgagccg cgcgctggtc gtctgcgccg accacgagct caacgcctcg gccttcgccg
     1981 tgcgcgtcgc ggcctcggcc ggcgccgatc tgtacgcctg cgtcagcgcc ggcctggcga
     2041 cgctgtccgg gccgcggcac ggcggcgtcg tcgatcgcgt cgaggcgctc gtcaccgagg
     2101 tcggtcgccc cgagcgcgcg caggaggtcg tgcaggagcg ggcccgtcgc ggcgaggagg
     2161 tgccgggctt tggccacccg ctgtaccccg cgggtgaccc ccgcgccgcg ctgttgctcg
     2221 ccgcggcgat cgagcacgcg ccccgcagcc cgcggctgcg cacgctcgag gcgatcgtcg
     2281 cggcgatgcg cgaggccggg cgcgagccgc cgaacctcga catcggcctc gcgtcgaccg
     2341 gcgtggcgct cggcatcccg ctgcgcacga gcgtcgcaat gttcgcggtc ggacgcgcgg
     2401 ccggctggat cgcccatgcc ttcgagcagc gcgccgccgg cttcctgctg cgtccgcgcg
     2461 cccgctacgt cggcccgtga tccgggcacg a
//
LOCUS       DASWNJ010001703         2462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_135515, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001703 DASWNJ010000000
VERSION     DASWNJ010001703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135515"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VGB85_28300"
     CDS             <1..517
                     /locus_tag="VGB85_28300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138033.1"
                     /translation="RARAARGRDGKVTRFHYAPLMIDLAYQVQVFRWAMIRPSFAIGG
                     NVANSRQAMPAAIQPGLFVGYQGALLGIAGGYTFNKAFPAIIGVNNGHGGPTQPVLDN
                     NHAIQGELSLTTRVDRGALNFAVRFGGVKSHLIHLDIDKKRWTGILTLSAGWFFDLGA
                     RRRRNTRAAGQ"
     gene            712..2097
                     /locus_tag="VGB85_28305"
     CDS             712..2097
                     /locus_tag="VGB85_28305"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HEY0138034.1"
                     /translation="MQKPLMKGAKPDIVAAIGDTPIVRLNHVTEGLESEIYVKLELLN
                     PAGSMKDRVGLNIIRDAEERGMLQPGGTIIEATSGNTGAGLAMVAATRGYKCIFVMPD
                     KMAREKVDTLRAYGAKVVICPTAVEPSDPRSYYSVSRRIVEETAGAFYANQYHNPANP
                     EAHYVSTAPEIWEQTGGDFDVFVSGMGTGGTLSGCGRYFKERKPEMKIVGVDPIGSLY
                     YEYVKTGRLTKPFSYYVEGIGEDFLPSTMNLNILDDIVQVDDRECFIMTRDLVRREGI
                     FAGGSCGAAVVGAIKYARTLKTPKRLLVLMPDGAQKYLSKIFNDEWMRQNGFFDEDSV
                     GGTVAHILRKKPGRVITASKTTTVREAIQMMKDHNISQLPILDEHQRHCGIVSELDLL
                     NYLVQNEGKLGEPLSDLINSDYATVSPHTRIQLLKSIFNDAKVVLVTEGDRMLGLLSK
                     IDLIDYLASQQ"
     gene            2102..>2462
                     /locus_tag="VGB85_28310"
     CDS             2102..>2462
                     /locus_tag="VGB85_28310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEY0138035.1"
                     /translation="MAIYDPSTDHLAAGAKLETLAIHGGQHPEPITGAVMPPISQAST
                     YAQKSPGEHSGFEYSRTHNPTRYALERAVASLEDGRFGLAFGSGMAATTTVLQMLSAG
                     DHIISGDDLYGGTFRLFD"
BASE COUNT          454 a          874 c          781 g          353 t
ORIGIN      
        1 ccgtgcccgc gcggcccggg gccgcgacgg caaggtgacg cgctttcact acgcgccgct
       61 gatgatcgac ctcgcgtatc aggtgcaggt gttccgctgg gcgatgatcc ggccgagctt
      121 cgcgatcggg ggcaacgtcg ccaactcgcg ccaggcgatg ccagcggcga tccagccggg
      181 cctgttcgtc ggctatcaag gcgccctgct cggcatcgcc ggcgggtaca ccttcaacaa
      241 ggccttcccg gcgatcatcg gggtcaacaa cggccacggc ggtccgaccc agccggtcct
      301 cgacaacaac cacgcgatcc agggcgagct ctcgctgacc acgcgggtcg atcgcggcgc
      361 gctcaacttc gcggtccgct tcggcggcgt gaagtcgcat ctgatccacc tcgacatcga
      421 caagaagcgg tggaccggga tcctcaccct cagcgccggg tggttcttcg atctcggcgc
      481 gcgccgccgg cgaaacacgc gagccgccgg acaatgacgg gcgggcgcgc cggccccggg
      541 cgaacctggc aaatccggcc aaaaccagcc aaacccagcc atgacggccg tttgtggcca
      601 gtctgccgct gttggcggtg ccccgggcgt gcgccgcctc gcgcacgccg ggcgaggagc
      661 gaggttctcc gccttcccgc gcgcgggcaa agcctctagc atcccggcac catgcaaaag
      721 ccgctgatga agggggccaa gcccgacatc gtcgccgcca tcggtgacac cccgatcgtc
      781 cgcctcaacc acgtcacgga gggcctcgag tccgagatct acgtgaagct ggagctgctc
      841 aacccggcgg gctcgatgaa ggaccgcgtc ggcctcaaca tcatccgcga cgccgaggag
      901 cgcggcatgt tgcagcccgg tggcacgatc atcgaggcga ccagcggcaa caccggcgcg
      961 ggcctggcga tggtcgcggc gacccgcggc tacaagtgca tcttcgtgat gcccgacaag
     1021 atggcgcgcg agaaggtcga caccctgcgc gcctacgggg ccaaggtggt catctgcccg
     1081 accgccgtcg agccgagtga tccgcgcagc tactactccg tctcgcggcg catcgtcgag
     1141 gagaccgcgg gggcgttcta cgccaaccag tatcacaatc cggccaaccc ggaggcgcac
     1201 tacgtctcga ccgcgccgga gatctgggag cagaccggcg gcgacttcga cgtgttcgtc
     1261 tcgggcatgg gcaccggcgg cacgctgtcg ggctgcgggc gctacttcaa ggagcgcaag
     1321 cccgagatga agatcgtcgg cgtcgacccg atcggctcgc tctactacga gtacgtcaag
     1381 accggccgcc tgaccaagcc gtttagctac tacgtcgagg gcatcggcga ggacttcctc
     1441 ccgtcgacga tgaacctcaa catcctcgac gacatcgtcc aggtcgacga ccgcgagtgc
     1501 ttcatcatga cccgcgacct cgtgcgtcgc gagggcatct tcgccggtgg ctcgtgcggc
     1561 gccgcggtcg tcggcgccat caagtacgcg cgcacgctca agacccccaa gcgcctgctg
     1621 gtgctgatgc ccgacggtgc gcagaagtac ctcagcaaga tcttcaacga cgagtggatg
     1681 cggcaaaacg gcttcttcga cgaggactcc gtcggcggca ccgtcgccca catcctgcgc
     1741 aagaagcccg gcagggtcat cacggcgagc aagacgacga cggtgcgcga ggcgatccag
     1801 atgatgaagg atcacaacat ctcgcagctg ccgatcctcg acgagcacca gcgccactgc
     1861 gggatcgtca gcgagctcga cctgctcaat tatctcgtgc aaaacgaagg caagctcggc
     1921 gagccgctga gcgacctgat caacagcgac tacgcgaccg tctcgccgca cacgcggatc
     1981 cagctgctca agagcatctt caacgacgcc aaggtggtcc tcgtgaccga gggcgaccgc
     2041 atgctcggcc tcctgagcaa gatcgacctg atcgactacc tcgcctcgca gcagtaagac
     2101 catggccatc tacgacccgt ccaccgacca cctcgccgcg ggcgcgaagc tcgagacgct
     2161 cgcgatccac ggcggccagc acccggagcc gatcaccggc gccgtgatgc cgccgatctc
     2221 gcaggcgagc acgtacgcgc aaaagagccc cggcgagcac agcggcttcg agtacagccg
     2281 cacgcacaat cccacccgct acgcgctcga gagagctgtc gcatcgctcg aggacggccg
     2341 cttcggcctc gcgttcggct ccggcatggc ggcgacgacg acggtgctgc agatgctcag
     2401 cgccggcgat cacatcatct cgggcgacga cctgtacggc gggacgtttc gcctgttcga
     2461 ca
//
LOCUS       DASWNJ010001704         1533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_596001, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001704 DASWNJ010000000
VERSION     DASWNJ010001704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596001"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="VGB85_28315"
     CDS             <1..1285
                     /locus_tag="VGB85_28315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138036.1"
                     /translation="PDDAITHLSRAAELGASTARFRLAQLLWSDFDLFDASDELDRYL
                     RAAGPYDLYWDAGRRLRERMDQVFLRIYLAIGALLLLLISIPAWQVYRRLRGASLAQL
                     LSRAPKSFPEVARILSLIRHEILKHNTAFLTDVGRALEMDEPDAEARAAIVARRLFGE
                     HRGGTPAHQPKVVRATGPSAGMRERGIYGRFLGYMAELQKVGRSHGVTLNLGRKDPIF
                     SAMIEAFEDLRGLEEMPRTPIRDRPTVKLDWSKRLTRSGHVLGRKAFEALSSVIHALC
                     IVEVDLALVREVFAQVVAETQFAGISILELQVVGEGARVRVFRSDLEDILTNVIRNSL
                     HSSILYAPKPIALGVALVTEMDEITGLSTLAIRIQDRSSEQLSNEMLRGRYVERGMGI
                     TADLLSRYDGSIAVEPEPGWQKAVVLRFFTVEEGP"
     gene            1282..>1533
                     /locus_tag="VGB85_28320"
     CDS             1282..>1533
                     /locus_tag="VGB85_28320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138037.1"
                     /translation="MNSNADPRELKVLIVEDGDEYLTNLSTFVARGIRYTQAKSGGWA
                     CTLLDTLRPDLVYLDMRFDRTPNEALLGDLVALTARFNGD"
BASE COUNT          233 a          567 c          492 g          241 t
ORIGIN      
        1 gcccgacgac gcgatcaccc acctcagccg cgccgccgag ctcggcgcct cgacggcccg
       61 ctttcgcctc gcgcagctgt tgtggtccga cttcgatctg ttcgacgcat cggatgagct
      121 cgaccgatac ctgcgcgccg cgggccccta cgacctctac tgggacgccg gccgccgcct
      181 gcgcgagcgc atggaccagg tatttttgcg gatctacctg gcgatcggcg cgctgctgct
      241 gctgctgatc tcgatccccg cgtggcaggt gtaccgccgc ctgcgcggcg cctcgctcgc
      301 gcagctgctg tcccgcgcgc ccaagagctt ccccgaggtc gcgcggatcc tgagccttat
      361 ccgccacgag atcctcaagc acaacaccgc gtttttgacc gacgtcggcc gcgcgctcga
      421 gatggacgag cccgacgccg aggcccgcgc ggcgatcgtc gcccggcggc tgttcggcga
      481 gcaccgcggc ggcaccccgg cgcaccagcc caaggtcgtg cgtgccacgg gcccgtccgc
      541 gggcatgcgc gagcgcggca tctacggccg cttcctcggc tacatggccg agctgcaaaa
      601 ggtcggtcgc agccacggtg tcaccctcaa cctcggccgc aaggacccga tcttcagcgc
      661 gatgatcgag gccttcgagg acctgcgcgg cctcgaggag atgccgcgca ccccgatccg
      721 cgatcggccg acggtcaagc tcgactggtc gaagcggctg acgcgatcgg gccacgtgct
      781 cgggcgcaag gccttcgagg cgctcagcag cgtcattcat gccctgtgca tcgtcgaggt
      841 cgacctcgcg ctggtccgcg aggtgttcgc gcaggtggtc gccgagacgc agttcgccgg
      901 gatctcgatc ctcgaattgc aggtcgtggg ggagggcgcc cgcgtgcgag tgttccgctc
      961 cgacctcgag gacatcctga ccaacgtgat ccggaattca ctgcactcga gcattctcta
     1021 cgcccccaag ccgatcgccc tcggcgtcgc gctggtcacc gagatggacg agatcaccgg
     1081 cctgagcacc ctggcgatcc gcatccagga ccgctcgagc gagcagctga gcaacgagat
     1141 gctgcgcggc cgctacgtcg agcgcggcat gggcatcacc gccgacctgc tgtcccgcta
     1201 cgacggatcg atcgccgtcg agcccgagcc cggctggcaa aaggccgtcg tgctgcgctt
     1261 cttcaccgtc gaggagggcc catgaattcg aacgcagacc cgcgcgagct caaggtgttg
     1321 atcgtcgagg acggcgacga gtacctgacc aacctctcga ccttcgtcgc ccgcgggatc
     1381 cgctacacgc aggccaagag cggcggctgg gcctgcaccc tgctggacac gctgcgcccc
     1441 gacctcgtgt acctcgacat gcgcttcgat cgcaccccga acgaggccct gctcggcgat
     1501 ctcgtcgcgc tgacggcccg cttcaacggc gac
//
LOCUS       DASWNJ010001705         4803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_700520, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001705 DASWNJ010000000
VERSION     DASWNJ010001705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700520"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..2120)
                     /locus_tag="VGB85_28325"
     CDS             complement(144..2120)
                     /locus_tag="VGB85_28325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEY0138038.1"
                     /translation="MTAAKLSRSTWSIAPVLAASLFAAPASAQGPMAGQPDPRAMSGL
                     PRVDPQTEPGTVVIRVLRGSFQNPGVGLTVTLDLRSADGSKTEQRTAVSVEQGRATFA
                     DLGAYAGGTAVASVDFGGEPAEVVKSQELRLDPNVGVRMLLVSGASSAPAAAAPVAGA
                     TATPAAADVPRPGTAFPNPGQARGSVVVGTLDLSGGKPFTATEVTLEIRKPDVPVEQR
                     KMTSDGRGAARFTGLADLPPGTTLVAEATLHGELKRSDPFSLDGQEHGVAVVLAVSSG
                     ASADTMGRPQRRPLQTPRAVPTVAPGIVRTTVFGPDDKPVGGVPVTVVKLDVTGTRRV
                     FTGEAGPDGVAFVEDIALADDSLYHVEVTHDKAPFRSRLFQMGDRMGVTLEMRVFPTT
                     SDVSKVRSAVQFGIEALENDQARVVQLHQALVEGDRAFWPATPLRIGGAEGATGMVVL
                     DRASVELEHAEGSPFATLREPLPPGEVVDMSIAYLMPHHGTLALRWSSPLPVANGRAV
                     VLPELKLKKGALGPPTRPPAQDGSGPSDFELYEIGAIPVGGSFDLEVEGLVTRPRTFR
                     ALGLALGLAIGLGALITLLVGPRASLQQRLERRRDVLLKTLDRLDAAPITDQSSAQRT
                     RVIAALDQVYRQLDVFKGSTAARVDLGAAWDRKA"
     gene            complement(2117..2776)
                     /locus_tag="VGB85_28330"
     CDS             complement(2117..2776)
                     /locus_tag="VGB85_28330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138039.1"
                     /translation="MHDPRRRIYLLAGGANVTALFVLAILRFGFGARLDAPTIALAVV
                     AAALVYALGQLYAMVWALSRPDRVAQVGQAAGSFTQAELRDEKRRLLRAIKELEFDFG
                     MGKLSKGDFDNVIATYRLRAIEVMRALEGGALHPDLAKLLAEREAARSAPAEAPAATD
                     ADRPRGPLDDTTRICGSCGGTNEADARFCKFCGTAMKKLDGTAVAPVQTPVAGEVGGA
                     P"
     gene            complement(2769..>4803)
                     /locus_tag="VGB85_28335"
     CDS             complement(2769..>4803)
                     /locus_tag="VGB85_28335"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEY0138040.1"
                     /translation="AVPTGGLSIATLGTATILVLFVLSLGTAALGALGAHRRSERLVE
                     GAVQGTYASFGLAAFASSLIIYAFLSGDYSIQYVQHTSDAAMPLFYKITAFWGGLDGS
                     LLFWVLLHTLFGSLAVRANRQRHRELIPHVVAVLATITCFFVGLLLFIKDPFSQFLLD
                     VPTQGKGLNPLLQNLYMIIHPPSLYLGYVSLAVPYAFGMAALFTGQVDAAWQRSVRRW
                     VLFSWMFLTFGLILGGLWAYEELGWGGYWAWDPVENAGLLPWFTATAFLHSVMIQERR
                     GMMKAWNLVLIIISFLLTIVGTFMTRSGVVQSVHAFGSDPVLAWTFGGFMLVIVLVSF
                     GLLIWRLPLLRSRGELESVMSREFAFLVNNWLFLICAIFVLTATMWPTFTEWRMILAE
                     RYPSWLAWQDPGERVVIGPPFFNLYMRPLGLALLLLTGIGPLLAWRKTSTESLIRQFG
                     GPLVFGAICTVVPRLFGIYSPWGLACFGLCGFTFWTIVQEFYRGVALRRKQRPQGIVE
                     ALISLVLRARRRYGGYIVHLGVVLMFLGWAGNAYKLEKKVQLRPGEEYTLGAYTIRHE
                     GLRATEDWQKEMITADLSVLKDGDVKDVLRPARWWYYQLPDQPTTEVSRYMTPGEDVY
                     VSMQEVDMTSGWTQLSLYINPLVNWIWIGMAVMLAGALVCAGTRKSEGAVDDA"
BASE COUNT          747 a         1663 c         1759 g          634 t
ORIGIN      
        1 cagcgtggtc ttgccggccc cgttggcgcc gacgaggccg acgatgctcg ccgccggcag
       61 ctcgaggtcg acgcgacgca gcaccggctg tcggccgaag cgctgcgaga cggcccgcag
      121 cgacacgacc gagcgcgcct cgctcatgcc ttgcggtccc aggcggcgcc gaggtcgacg
      181 cgcgccgcgg tggagccctt gaacacgtcg agctggcggt agacctggtc gagcgcggcg
      241 atcacccgcg tgcgctgggc cgaggactga tcggtgatcg gcgcggcgtc gaggcgatcg
      301 agggtcttga gcaggacatc gcggcgtcgc tcgaggcgct gctgcaggct cgcgcgcgga
      361 ccgacgagca gggtgatcag cgcgccgagg ccgatcgcca ggccgagggc gaggccgagg
      421 gcgcggaagg tgcgaggccg cgtgacgagg ccctcgacct cgaggtcgaa gctgccgccg
      481 acggggatcg caccgatctc gtagagctcg aagtccgagg gccccgagcc gtcctgcgcg
      541 ggcggccgcg tcggcgggcc gagggcgccc ttcttgagct tgagctcggg cagcacgacg
      601 gcgcgcccgt tggcgaccgg cagcggcgag gaccagcgca gcgcgagcgt gccgtgatgc
      661 ggcatcaggt acgcgatcga catgtcgacg acctcgcccg gcggcagcgg ctcgcgcagc
      721 gtggcgaagg gtgagccttc ggcgtgctcg agctcgacgc tcgcgcggtc gagcacgacc
      781 atcccggtcg cgccctcggc cccgccgatc cgcagcggcg tggccggcca aaacgcccgg
      841 tcgccctcga cgagcgcctg atgcagctgg acgacgcgcg cctggtcgtt ctccagcgcc
      901 tcgatgccga actgcaccgc gctgcggacc ttgctgacgt cgctcgtcgt cgggaacacg
      961 cgcatctcga gcgtgacacc catgcggtcg cccatctgga acaggcgcga gcgaaacggc
     1021 gccttatcgt gggtgacctc gacgtggtag aggctgtcgt cggcgagcgc gatgtcctcg
     1081 acgaaggcga cgccgtccgg cccggcctcg ccggtgaaga cgcgtcgcgt gccggtgacg
     1141 tcgagcttga cgaccgtgac cggcacgccg ccgactggtt tgtcgtcggg gccgaacacg
     1201 gtggtgcgca cgatgccggg ggcgaccgtc ggcaccgcgc gcggggtctg cagcgggcgt
     1261 cgctgcggcc ggcccatcgt gtccgcggag gcgccgctgc tgaccgcgag cacgacggcg
     1321 acgccgtgct cctggccatc gaggctgaag gggtccgagc gcttgagctc gccgtgcagc
     1381 gtcgcctcgg cgacgagcgt cgtgccgggc gggaggtcgg cgaggccggt gaagcgcgcc
     1441 gcgccgcgac cgtcgctcgt catcttgcgc tgctcgaccg ggacgtcggg cttgcggatc
     1501 tcgagggtga cctcggtggc ggtgaacggc ttgccgccgc tgaggtcgag cgtgccgacg
     1561 accaccgagc cgcgcgcctg gccggggttc gggaacgcgg tgccggggcg cggcacgtcg
     1621 gccgccgcgg gcgtcgccgt cgcgcccgcg accggcgcgg ctgctgcggg ggcactgctc
     1681 gcgccggaca ccagcaacat ccgcacgccg acgttcgggt cgaggcggag ctcctgcgac
     1741 ttgacgacct cggccggctc gccgccgaag tcgacgctgg cgaccgcggt gccgcccgcg
     1801 tacgcgccga ggtcggcaaa ggtcgccctg ccctgctcga ccgatacggc cgtgcgctgc
     1861 tcggtcttgc tgccgtcggc gctgcgcagg tcgagggtga cggtcaggcc gacgccgggg
     1921 ttctggaacg acccgcgcag cacccggatc accacggtgc cgggctccgt ctggggatcg
     1981 acgcgcggca ggccggacat cgcccgcggg tcgggctggc cggccatcgg cccctgcgcc
     2041 gacgcaggcg cggcgaacag gctggccgcg aggacaggtg cgatcgacca ggtgcttcgg
     2101 gacagcttcg cggcggtcat ggtgcccctc cgacctcgcc ggcgaccggc gtctggaccg
     2161 gcgccacggc ggtgccgtcg agcttcttca tcgccgtccc gcagaattta cagaagcgag
     2221 cgtcggcctc gttggtgccg ccgcagctgc cgcagatccg cgtcgtgtcg tcgagcgggc
     2281 cgcgcgggcg gtccgcgtcg gtcgcggccg gcgcctcggc gggcgcgctg cgggccgcct
     2341 cgcgctcggc gagcagcttc gccagatccg ggtgcagggc gccgccctcg agcgcgcgca
     2401 tgacctcgat cgcgcgcagg cgataggtcg cgatcacgtt gtcgaagtct cccttcgaca
     2461 gcttgcccat gccgaagtcg aactcgagct ccttgatcgc gcggagcaac cttcgcttct
     2521 cgtcgcgcag ctcggcctgg gtgaagctgc cggccgcctg gccgacctgc gcgacgcggt
     2581 cgggccggga cagcgcccag accatcgcgt agagctggcc gagcgcgtac accagcgccg
     2641 cggcgacgac cgcgagggcg atcgtcgggg cgtcgagccg ggcgccgaag ccgaacctga
     2701 ggatcgccag gacgaagagc gccgtgacgt tcgccccgcc ggccagcagg taaatgcggc
     2761 gtcgcgggtc atgcatcgtc gaccgcgcct tccgacttgc gggtcccggc gcacaccagc
     2821 gcgcccgcga gcatcaccgc catgccgatc cagatccagt tgaccagcgg gttgatgtag
     2881 aggctcagct gcgtccagcc gctggtcatg tcgacctcct gcatcgacac gtacacgtcc
     2941 tcgcccgggg tcatgtagcg cgacacttcg gtcgtcggct gatcggggag ttgatagtac
     3001 caccagcgag ccggccgcag cacgtccttg acgtcgccgt ccttgaggac cgacaggtcc
     3061 gcggtgatca tctctttttg ccagtcctcg gtcgcgcgca ggccctcgtg gcggatcgtg
     3121 taggcgccga gcgtgtactc ctcgcccggc cgcagctgga ccttcttctc gagcttgtag
     3181 gcgttgccgg cccagccgag gaacatcagc acgacgccga ggtgcacgat gtagccgccg
     3241 tagcgccggc gtgcgcgcag caccagcgag atcagcgcct cgacgatgcc ctgggggcgc
     3301 tgcttccggc gcagcgcgac gccgcggtag aactcctgca cgatcgtcca gaaggtgaag
     3361 ccgcacaggc cgaagcaggc caggccccac ggcgagtaga tgccgaacag ccgcggcacg
     3421 accgtgcaga tcgctccgaa caccagcggg ccgccgaact ggcggatgag gctctcggtc
     3481 gaggtcttgc gccacgcgag cagcgggccg atgccggtca gcagcagcag cgcgagcccg
     3541 agcggccgca tgtacaggtt gaagaacggc gggccgatga cgacgcgctc gcccgggtcc
     3601 tgccacgcga gccacgacgg gtagcgctcg gcgaggatca tgcgccactc cgtaaaggtc
     3661 ggccacatcg tcgcggtgag cacgaagatc gcgcagatca ggaacagcca gttgttgacc
     3721 aggaacgcga actcgcggct catcacgctc tcgagctcgc cgcgcgagcg cagcagcggg
     3781 aggcgccaga tcagcagccc gaagctgacg agcacgatga cgagcatgaa gccgccgaag
     3841 gtccacgcga gcaccgggtc ggagccgaag gcgtgcacgc tctggacgac gccgctgcgg
     3901 gtcatgaagg tgccgacgat cgtcagcaag aagctgatga tgatcaggac caggttccac
     3961 gccttcatca tgccgcggcg ctcctggatc atcaccgagt gcaggaacgc ggtcgcggtg
     4021 aaccacggca gcaggcccgc gttctcgacc ggatcccagg cccagtaacc gccccagccg
     4081 agctcctcgt aggcccacag acccccgagg atcaggccga aggtcaggaa catccacgag
     4141 aacagcaccc agcgccgcac gctgcgctgc cacgcggcgt cgacctggcc ggtgaacagc
     4201 gccgccatgc cgaacgcgta aggcaccgcg agcgagacgt agccgaggta caggctcggc
     4261 gggtggatga tcatgtacag gttctgcagc agcgggttga ggcccttgcc ctgcgtcgga
     4321 acgtcgagca aaaactgcga gaacgggtct ttaataaaaa gcagcaggcc gacgaagaag
     4381 caggtgatcg tggcgagcac cgcgacgacg tgcgggatca gctcgcggtg gcgctgccgg
     4441 ttggcgcgca cggcgagcga gccgaacagc gtgtgcagca gcacccagaa cagcaggctg
     4501 ccgtcgaggc cgccccagaa cgcggtgatc ttgtagaaca gcggcatcgc cgcgtcgctg
     4561 gtgtgctgca cgtactggat cgagtagtcg cccgacagga acgcgtagat gatcagcgac
     4621 gaggcgaagg cggcgagccc gaagctcgca taggtgccct gcaccgcgcc ctcgacgagg
     4681 cgctcggatc gccggtgggc cccgagcgcg ccgagagccg cggtcccgag gctgagcacg
     4741 aacaggacga ggatcgtcgc cgtgccgagc gtggcgatcg acaggccgcc ggtcggcacg
     4801 gcc
//
LOCUS       DASWNJ010001706         5368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_562776, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001706 DASWNJ010000000
VERSION     DASWNJ010001706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562776"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VGB85_28340"
     CDS             <1..513
                     /locus_tag="VGB85_28340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138041.1"
                     /translation="RERTCMDARMRQPGFFPSFSSQEVEASPQAPVQVSREMLFTQTP
                     APTAGTAGTVPTGAERRQSGARVRARDRLMDRMLRPAQATDPLSRVRGVENLVLFIDD
                     DLRETAMALGNVEGYLLEILKLLEAPRLAREEVHALAADTQVLDHVDMLVETLETLRR
                     RLAKLSASLR"
     gene            571..1383
                     /gene="mrtZ"
                     /locus_tag="VGB85_28345"
     CDS             571..1383
                     /gene="mrtZ"
                     /locus_tag="VGB85_28345"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_172302887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MrtZ family glutamic-type intramembrane
                     protease"
                     /protein_id="HEY0138042.1"
                     /translation="MRVRRARARRAVAATGILYASYGLLGLVPGLADYRGVALVAGFY
                     FLPAWLLRQEPEVQLRYQVGPDSPVPPWRWRGLWVAVAAALLLFPPFVLCFWWFYHHV
                     CAGDLRVLSPVLWAEGLTPYAGGLERFLGSLCRTHAGGFWPDGLHFPASWTAWYGLGF
                     VRQVAEGLFAVALAEEVFHRGYLMSALEDLWPPRRRVLGVPFGLAAVVSSLLFAVGHL
                     VGMAQAARLATFFPALVFAWLWRRSGSLWAPALFHTAANLLMDLLLASTFPT"
     gene            1380..2087
                     /locus_tag="VGB85_28350"
     CDS             1380..2087
                     /locus_tag="VGB85_28350"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY0138043.1"
                     /translation="MSAAHDQREDVAPPAAGKVGAFFDVDHTLLDVNSGARWLRHAWR
                     TGQTSSLQALRALGWLVRYRLALLDLEAVSALVARDYRGYSAADLDAEARSWFRRDIE
                     AHVCIEGRARVADHLAKGHVVALLTSGTRFSVTPLAELLGVTHLLCTELEVEAGKLTG
                     RHIAPACAGAGKVVYGERFAAEHGIDLSRSYFYTDSYSDLPMLERVGCPRVVNPDPRL
                     KQRARSRGWEIERWTAY"
     gene            2072..2935
                     /gene="nadC"
                     /locus_tag="VGB85_28355"
     CDS             2072..2935
                     /gene="nadC"
                     /locus_tag="VGB85_28355"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEY0138044.1"
                     /translation="MDSILSEIGPIAGIIDAAIAEDLASGDVTGRVTVPAHTRWQARL
                     VAKDKLVVAGGAVAKAVFHRVDPEITAEVHLRDGEVAAPGTVLMDLEGNARALLAAER
                     TALNLFQRMCGVATVTRAYVDAAAGRCRICDTRKTMPGLRALDRYAVRCGGGHNHRND
                     LGAGVLIKENHVRAAGGIGEAIARARVHAPHTLRIECEVTSLDELREALTAGADAVLL
                     DNMNDAQIREAVAIVAGKAIIEVSGGISLDRITLLASVGIDVISVGKLTHSAPASDIS
                     LLFQLGDGRWG"
     gene            2935..3723
                     /locus_tag="VGB85_28360"
     CDS             2935..3723
                     /locus_tag="VGB85_28360"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY0138045.1"
                     /translation="MTTRLSTAVLGQVHEHHASLPSTNDRALAWAREGAPHGALVTAD
                     EQTAGRGRLGRRWVSPAGEGLYASVVLRPESFLNGLTGGAGWSPRWSALGLAVGLGVR
                     MGISRWLPETRLKWPNDLLVDRRKLAGVLCETRWQGATPDVVVGFGVNVLQREFPEEL
                     RATSMALELAHETCPSRHEVLEAALLGLEGVLGRFFAGGFPAIRGQYEAHSAVIGQAL
                     TVGGVAAVGVGFDDDGALRVRTSGEASWRVESEDVWLREPGRAE"
     gene            3720..4409
                     /locus_tag="VGB85_28365"
     CDS             3720..4409
                     /locus_tag="VGB85_28365"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0138046.1"
                     /translation="MTTVLVLLPGMDGTGRMFAPLLARLPATLDARPIAYPCDRELGY
                     AELLGLVRQQLPRGDFFVLAESFSGPLALRLAGERPRGLCGLILAASFAASPLAWLPR
                     WSHGLAHARLFGGLGMGPRLALGLGRCPPAITPMLRDALALVSPAVMARRAREVLRCE
                     APLNREALAELPALLLGAERDRLIGRRHVGALQALLPRAQVEWFDAPHLLLQARPDEA
                     AEKIAAWIGRS"
     gene            complement(4406..>5368)
                     /locus_tag="VGB85_28370"
     CDS             complement(4406..>5368)
                     /locus_tag="VGB85_28370"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: protein motif:HMM:NF016822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HEY0138047.1"
                     /translation="ARVVGTSMIHAQHGDFDEPHVFFRVMQEERYADLRVTEDAEVHD
                     VHMTHTMLYLGQTYDGPTELGGLVLHPDLRRHPDKLGRLLSLARFVFIAAFRGWFRDR
                     LLAELLPPLYKGPGGTRSPLWDALGHKFTGLSYDEADRLSRFNKEFIWRLFPTMPVHA
                     SMLPDEVQEIIGQVGPNTVGAQRLLESIGFQYSGRVDPFDGGPHYEADTDQVTFVRDT
                     RSYQPIVGEPDDTAQPGIVAVTADRSPHFRAVWTPVQAVHDPDDPGQPTLVRVGEAAL
                     ARLGVTADEARVLVALRPQRRHSDRTSRVFPPARAPTGSLRTGG"
BASE COUNT          667 a         1685 c         2165 g          851 t
ORIGIN      
        1 cgcgagcgga cgtgcatgga tgcccgcatg cgccagcccg gcttcttccc ctcgttctcc
       61 tcgcaagaag tcgaggcctc gccccaggca cctgtgcagg tgtccaggga gatgctgttc
      121 acgcagacgc cggcgccgac ggcggggacg gcggggacgg tgccgacggg cgcggagcgg
      181 cggcagagcg gtgcgcgcgt gcgcgcacgt gaccgtctga tggaccgtat gctgcgtccg
      241 gcgcaggcga cggacccgct gagccgggtg cgcggcgtcg agaacctggt gctgttcatc
      301 gacgacgacc tgcgcgagac ggcgatggcg ctcggcaacg tcgagggtta cctgctggag
      361 atcttgaagc tgctcgaggc gccgcggctg gcccgtgagg aggtccacgc gctcgccgcc
      421 gacacgcagg tgctcgacca cgtcgacatg ctcgtcgaga cgctcgagac cctgcgccgc
      481 cggctggcga agctctcggc gagtctgcgc tagagttcgc ggccgttggt ctcgccgagc
      541 acagcgccgg gcaccccgga ccccgcggac ctgcgagtcc gcagggcccg cgcgcggcgt
      601 gccgtggctg cgacggggat cttgtatgcg agttatgggc tgctcggact ggtcccgggg
      661 ctggcggact accgcggggt cgcgctggtg gccgggttct attttttgcc ggcctggctc
      721 ttgagacagg agccggaggt gcagctgcgc taccaggtcg ggcccgactc gccggtgccg
      781 ccgtggcgct ggcgcgggct gtgggtcgcg gtcgcggcgg cgctgctgct gttcccgccg
      841 ttcgtgctgt gcttctggtg gttctatcac cacgtctgcg ccggcgacct gcgggtgctg
      901 tcgccggtgc tgtgggcgga ggggctgacg ccgtacgcgg gcgggctcga gcggttcctg
      961 gggtcgctgt gtcgcacgca cgcgggcggg ttctggcccg acgggctgca cttcccggcg
     1021 agctggacgg cgtggtacgg gctcggcttc gtgcgtcagg tggccgaggg gctgttcgcg
     1081 gtggcgctcg cggaggaggt gtttcatcgc ggatatctca tgagcgcgct ggaagacctg
     1141 tggccgccgc gacgaagggt gctcggcgtg cctttcgggc tcgcggcggt ggtctcgagc
     1201 ctgctgttcg cggtcgggca cctggtcggg atggcccagg ctgcgcgcct ggcgacgttc
     1261 ttcccggcgc tcgtgttcgc gtggctgtgg cggcgcagcg gcagcctgtg ggctcccgca
     1321 ctgtttcata cggcggcgaa cttgttgatg gacctgctgc tggccagtac attcccgaca
     1381 tgagcgcggc gcacgaccag cgagaggatg tcgcgccgcc cgcggcgggc aaggtcggcg
     1441 ccttcttcga tgtcgaccac acgctgctcg acgtcaactc gggggcgcgg tggctgcgac
     1501 acgcgtggcg caccgggcag acctcgagcc tgcaggccct gcgcgcgctg ggctggctgg
     1561 tccgctaccg cctggcgctg ctcgacctcg aggccgtgtc ggcgctggtg gcgagggact
     1621 atcgcggata ctcggcggcc gacctcgacg cggaggcgcg ctcatggttt cgccgcgaca
     1681 tcgaggcgca cgtgtgcatc gagggccgcg cgcgcgtggc ggaccacctg gcgaaggggc
     1741 acgtggtcgc gctgttgacc tcgggcacgc gcttttcggt gacgccgctg gcggagctgc
     1801 tcggggtgac gcacctgctg tgcaccgagc tcgaggtcga ggcgggcaag ctgaccgggc
     1861 ggcacatcgc gccggcctgc gcgggcgcgg gcaaggtcgt gtacggcgag cgcttcgccg
     1921 ccgagcacgg gatcgacctg tcccggagtt acttttatac ggacagctac agcgacctgc
     1981 cgatgctcga gcgggtcggc tgtcctcgcg tcgtcaatcc ggacccgcgc ctgaaacaac
     2041 gcgcgcgcag ccggggctgg gagatcgaga gatggacagc atactgagcg agatcgggcc
     2101 catcgcgggc atcatcgacg cggcgatcgc cgaggacctc gccagcggcg acgtcacggg
     2161 tcgcgtcacc gtgccggcgc acacccgctg gcaggccagg ctggtcgcca aggacaagct
     2221 ggtggtcgct ggcggggcgg tcgcgaaggc ggtgtttcat cgcgtcgacc ccgagatcac
     2281 ggccgaggtg catctgcgcg acggcgaagt ggcagcgccg ggcacggtgc tgatggacct
     2341 cgagggcaac gcgagggcgc tgctggcggc cgagcgcacg gcgctcaacc tgttccagcg
     2401 catgtgtggg gtcgcgacgg tgacgcgggc gtatgtcgac gcggcggccg gtcgctgccg
     2461 gatctgcgac acgcgaaaga ccatgccggg gctgcgcgcg ctcgatcgct acgcggtgcg
     2521 ctgcggcggc gggcacaacc atcgcaacga cctcggcgcc ggcgttttga ttaaagagaa
     2581 ccacgtgcgc gcggcgggcg gcatcggcga ggcgatcgcg cgggcccggg tccacgcgcc
     2641 gcacacgctg cgcatcgagt gcgaggtgac gagcctcgac gagctgcgcg aggcgctgac
     2701 ggccggcgcg gacgcggtgc tgctcgacaa catgaacgac gcgcagatcc gcgaggcggt
     2761 ggcgatcgtc gcgggcaagg cgatcatcga ggtcagcggt gggatcagcc tcgatcgcat
     2821 cacgctgctg gcgagcgtcg ggatcgacgt catcagcgtc gggaagctga cccacagcgc
     2881 gccggcgagc gacatctcgc tgctgttcca gctcggcgac ggtcgctggg gttgatgacg
     2941 acgcggctgt ccacggcggt gctcgggcag gtccacgagc accacgcgag cctgccttcc
     3001 acaaatgatc gcgcgctcgc gtgggcccgg gaaggggcgc cccacggggc cttggtgacc
     3061 gccgacgagc agacggccgg gcgcgggcgc ctcgggcggc gctgggtctc gcccgcgggc
     3121 gaggggctgt atgccagcgt ggtgctgcgg ccggagtctt ttttaaatgg gttaacgggc
     3181 ggggcggggt ggtcgccgcg ctggtcggcg ctcgggcttg cggtcgggct cggcgtgcgc
     3241 atggggatct cgaggtggtt gccggagacg cggctcaagt ggcccaatga tttgttggtc
     3301 gatcggcgca agctcgcggg ggtgctgtgt gagacccgct ggcagggggc gacgccggat
     3361 gtggtggtcg gcttcggggt caatgtgctt cagcgggagt ttccggagga gctgcgggcg
     3421 acgagcatgg cgctcgagct ggctcatgag acatgtccct cgcgacatga ggtgctcgag
     3481 gcggcgttgc tcgggctcga gggggtgctg ggacgttttt ttgccggcgg ctttccggcg
     3541 atccgcgggc agtacgaggc tcacagcgcg gtgatcgggc aggcgctgac ggtcggcggg
     3601 gttgcggcgg tgggggtcgg cttcgatgac gacggggcgc tgcgcgtgcg gacgtccggt
     3661 gaggcgagct ggcgcgtcga gtccgaggac gtgtggctgc gggagcccgg gcgggccgaa
     3721 tgacgacggt gttggtgttg ctgccgggca tggacgggac cgggcggatg ttcgcgccgt
     3781 tgctcgcgcg gctgccggcg acgctcgacg cgcggccgat cgcctatccc tgtgaccgcg
     3841 agctcgggta cgccgagctg ctcgggctgg tccggcagca gctcccgcgg ggggacttct
     3901 ttgtgctcgc cgaatcgttc tcggggccgc tggcgctgcg gctggctggc gagcgcccac
     3961 gagggttgtg cgggctgatc ctcgcggcga gcttcgctgc gagcccgctc gcgtggcttc
     4021 cacgctggtc gcacgggctc gcgcatgcgc gtctgttcgg cggcctcgga atgggtcccc
     4081 ggctcgcgct ggggctcggg cgctgtccac ccgcgatcac gccgatgttg cgcgacgcgc
     4141 tcgcgctcgt ctcaccggcg gtgatggccc gacgggcgcg ggaggtcctg cgctgcgagg
     4201 cgccgctgaa ccgcgaggcg ctggccgagt tgccggcgct gctcctcggc gcggagaggg
     4261 accgactgat cggccgccgg catgtcggcg cgctgcaggc gttgctgccg cgcgcgcagg
     4321 tcgaatggtt cgacgcgccg catcttctcc ttcaggcgcg accggacgag gccgcggaga
     4381 agatcgccgc gtggatcggg cggtcctagc cgcccgtgcg cagggagccg gtcggggcgc
     4441 gggcgggggg gaagacacgg ctggtgcggt cgctgtggcg gcgttgtgga cgcagggcga
     4501 cgagcacgcg ggcctcgtcg gcggtgacac cgagtcttgc gagcgcggcc tcgccgacgc
     4561 gcacgagggt cggctggccg gggtcgtcgg ggtcgtgcac ggcctgaacg ggggtccaga
     4621 cggcgcgaaa gtggggcgag cggtcggcgg tgacggcgac gatgccgggc tgggcggtgt
     4681 cgtcgggctc gccgacgatc ggctggtagc tgcgggtgtc gcggacgaag gtgacctggt
     4741 cggtgtcggc ctcgtagtgc gggccgccgt cgaaggggtc gacgcggccg ctgtactgga
     4801 agccgatgct ctcgagcagg cgctgcgcgc cgacggtgtt gggcccgacc tgaccgatga
     4861 tctcctggac ctcgtcgggc agcatgctcg cgtgcaccgg catcgtcggg aacaggcgcc
     4921 agatgaactc tttgttgaaa cggctcaggc ggtcggcctc gtcgtagctg aggccggtga
     4981 acttgtggcc gagcgcgtcc cacagcggcg agcgcgtgcc gcccgggccc ttgtagaggg
     5041 gtggcagcag ctcggcgagc aagcggtcgc ggaaccaccc gcgaaacgcg gcgatgaaca
     5101 cgaagcgggc cagcgagagc aggcggccga gcttgtcggg gtggcggcgc aggtcggggt
     5161 ggagcacgag gccgccgagc tcggtcggac cgtcgtaggt ctggccgagg tagagcatcg
     5221 tgtgggtcat atgcacgtcg tggacctcgg cgtcctcggt gacgcgcagg tcggcgtagc
     5281 gctcctcctg catgacgcgg aagaacacgt gcggctcgtc gaagtcgccg tgctgggcgt
     5341 ggatcatcga ggtgccgacg acgcgggc
//
LOCUS       DASWNJ010001707         2952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_562844, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001707 DASWNJ010000000
VERSION     DASWNJ010001707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562844"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VGB85_28375"
     CDS             <1..449
                     /locus_tag="VGB85_28375"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HEY0138048.1"
                     /translation="LYGAELLQNEVVEPAATVGDPPARLRAMAQNYVVFARTHRWLYS
                     VMMEQAQLDWSAMAPEELELTLRPLRTCQQWLRDGAELACWRRGLNIEAAAFRLWLSM
                     HGLCSMLNSGRIDERHPVFPFADQDTFVTHFIDDTIQALCADPVAA"
     gene            complement(513..2405)
                     /locus_tag="VGB85_28380"
     CDS             complement(513..2405)
                     /locus_tag="VGB85_28380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138049.1"
                     /translation="MQRVEPRLTAELRARLARGEHEAVASELASGGDLVGAGQVLEQI
                     WDFLGAHGHYTAAGRPLDALRCALELGRPDLLDATLAQLMDQPRGDRDEAVKLLQRRG
                     RHAEAAGLLATDDADPVVRARALARAGDRRGAAQVLAEAGHVREALRALDPIAEQDAP
                     ALTLAATLAWELGDAETSVRHAQAAMRALAGPATHDPGSAGPRASQPALAAMSEGTAT
                     DERANAARILARGLGALGHELAAQLVLQATGVREMTSAAVPGRYHVRRALTASYAGAA
                     YDGLDRVTQQEVEIHLLLAEVQDAGLDAQGVLSAIAAFARRAEAAAELAHPAIRPVLR
                     FDLRAGLLILPHADGPTLRTLIRPPGMPPARARALVSFLLDGLVAAHARGLVHGSLLP
                     AQIVCDAAGRPLLGPFGADEIAGLVATRTGALEELLTITAPELRAGGKPSAASDIYSA
                     AALWVALRTGSLGADPARLPADEQRLVADALDPDPARRPDAPTLLGRLRVRVADPREL
                     VPLAPLPAVPKAMSEVGALATAGVIAEVAAGWTDAELDALLASDAPRLQPVLDREQRR
                     VVLAAWPDGCRRLADDADAGALDPLLDGLAPALRAIVGSRLRASSWVCTSAGEWMLAL
                     DDLLTR"
     gene            complement(2410..>2952)
                     /locus_tag="VGB85_28385"
     CDS             complement(2410..>2952)
                     /locus_tag="VGB85_28385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138050.1"
                     /translation="GRGDVVSAVLTVASPHRGTYLTDVALGLAPGAAEDLLGALLNFV
                     GALGSQQKSDAKASFYSLSEAYMQGEFNPQNPDDPRVKYISYTGRTCDALAYLDPENN
                     CQELCDPLIAWAYRMLKETRGANDGLVTVESAKWGEYRGEMIADHIDEVGQIAGITDI
                     KFDHLAFYRDRVRDLAAEQH"
BASE COUNT          363 a         1047 c         1151 g          391 t
ORIGIN      
        1 ggctctatgg cgcggagctc ctgcaaaacg aggtcgtcga gcccgccgcg accgtcgggg
       61 atccgccggc tcgcctgcgc gcgatggccc aaaactatgt cgtgttcgcc cgcacccatc
      121 gctggctgta ctcggtgatg atggagcagg cccagctcga ctggtctgcg atggcgccgg
      181 aggagctcga gctgacgctg cgtccgctgc ggacctgcca gcagtggctg cgcgacggcg
      241 ccgagctcgc ctgctggcgc cgcggcctca acatcgaggc cgccgcgttc cgcctgtggc
      301 tgtcgatgca cggcctgtgc tcgatgctca actcgggtcg catcgacgag cgccacccgg
      361 tgttcccgtt cgccgatcag gacaccttcg tcacgcactt catcgacgac acgatccagg
      421 cgctgtgcgc cgatccggtc gccgcctgag ctctattgat caagccgtga agcggcgcgc
      481 gaggcctccg tgcccccgcg cgccgtcgtg tatcagcggg tcagcaggtc gtcgagcgcg
      541 agcatccact cgcccgcgga cgtgcacacc cacgaggacg cgcgcagccg cgagccgacg
      601 atcgcacgca gcgccggagc gaggccgtcg agcagcggat cgagcgcgcc cgcgtcggcg
      661 tcgtcggcga gccggcggca accatcgggc cacgcggcga gcacgacgcg ccgctgctcg
      721 cggtcgagca ccggctgcaa tcgcggggca tcacttgcga gcagcgcgtc gagctcggcg
      781 tcggtccagc ccgccgcgac ctccgcgatg acgcccgcgg tcgccagcgc gcccacttct
      841 gacatggcct tcgggacagc cggcagcggc gccagcggga ccagctcgcg tggatcggcg
      901 acgcgcacgc gcaggcgccc gagcagcgtc ggtgcgtccg gacgtctcgc ggggtcgggg
      961 tcgagggcgt cggcgacgag tcgctgctcg tcggcgggca ggcgagccgg gtcggcgccg
     1021 agggatccgg tgcgcagcgc gacccacagg gccgcggcgc tgtagatgtc actcgcggcg
     1081 ctgggcttgc cgccggcccg cagctcgggc gcggtgatcg tcagcagctc ctcgagcgcg
     1141 ccggtgcggg tggcgacgag gcccgcgatc tcgtcggcgc caaacggccc gagcagcggg
     1201 cggcctgccg cgtcgcacac gatctgcgcc ggcagcagcg agccgtgcac gagcccgcgg
     1261 gcgtgcgccg cgacgaggcc gtcgagcagg aagctgacga gcgcgcgggc gcgggccggg
     1321 ggcatgcccg gcggtcggat gagcgtgcgc agcgtgggcc cgtcggcgtg agggaggatc
     1381 agcagaccgg cgcgcaggtc gaagcgcagc accgggcgga tcgcggggtg cgccagctcg
     1441 gccgccgcct cggcccgccg ggcgaacgcg gcgatcgccg acagcacgcc ctgggcgtcg
     1501 aggccggcgt cctggacctc ggcgagcagg aggtggatct cgacctcctg ttgcgtgacg
     1561 cggtcgaggc cgtcgtaggc ggcgccggcg tagctggcgg tcagcgcccg ccgcacgtgg
     1621 tagcgcccgg gcaccgcggc gctcgtcatc tcgcgcacgc cggtcgcctg caacacgagc
     1681 tgggcggcga gctcgtgacc gagcgccccg aggccgcgcg cgaggatccg cgccgcgttc
     1741 gcgcgctcgt cggtggctgt cccttcggac atggccgcaa gggcaggctg ggacgcccgc
     1801 ggtccggcgc tgccagggtc gtgcgtcgcg ggtccggcga gcgcgcgcat cgccgcctgc
     1861 gcgtggcgga cgctggtctc ggcgtcgccg agctcccacg cgagcgtcgc ggcgagcgtc
     1921 aacgcgggcg cgtcctgctc ggcgatcggg tcgagggcgc gcagcgcctc gcgcacgtgg
     1981 ccggcctcgg cgagcacctg cgcggccccg cggcggtcgc cggcgcgggc gagggcgcgg
     2041 gcgcggacga ccgggtcggc gtcgtccgtg gccagcaagc cggcggcctc ggcgtggcgc
     2101 ccacggcgtt gcaggagctt gacggcctcg tcccggtcgc cgcgcggctg gtccatcagt
     2161 tgcgcgagcg tggcgtcgag caggtcgggg cggccgagct cgagcgcgca gcgcagggcg
     2221 tcgagcgggc gtccggcggc cgtgtagtgg ccgtgcgcgc cgaggaagtc ccagatctgc
     2281 tcgagcacct gaccggcgcc gacgaggtcg ccgccggacg cgagctcgct ggcgacggcc
     2341 tcgtgttcgc cacgcgcgag gcgagcgcgc agttccgcgg tcaaccgtgg ctcgacgcgt
     2401 tgcacggcct cagtgctgct cggccgcgag gtcgcggacg cggtcgcggt aaaacgcgag
     2461 gtggtcgaac ttgatgtcgg tgatgccggc gatctggccg acctcgtcga tgtggtcggc
     2521 gatcatctcg ccgcggtact cgccccactt cgcgctctcg acggtgacga ggccgtcgtt
     2581 ggccccgcgg gtctccttca acatgcgata ggcccaggcg atcagcgggt cgcacagctc
     2641 ctggcagttg ttctcggggt cgaggtacgc cagggcgtcg caggtgcggc cggtgtacga
     2701 gatgtacttg acgcgcgggt cgtccgggtt ctgcgggttg aactcgccct gcatgtacgc
     2761 ctcgctcagc gagtagaacg aggccttggc gtccgacttc tgctgcgagc cgagtgcgcc
     2821 gacgaagttg agcagcgcgc cgagcaggtc ctcggccgcc ccgggcgcga ggcccagcgc
     2881 gacgtcggtc aggtaggtgc cgcggtgcgg gctggcgacg gtcagcacgg cggagacgac
     2941 gtcgccgcgg cc
//
LOCUS       DASWNJ010001708         2095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_170475, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001708 DASWNJ010000000
VERSION     DASWNJ010001708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170475"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..1793
                     /locus_tag="VGB85_28390"
     CDS             102..1793
                     /locus_tag="VGB85_28390"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0138051.1"
                     /translation="MSRPRRSLALLILTACGPAEAPAPQPEPAATPVTLTIVGTSDLH
                     GHLRALPLLAGHLNGLRRARANDGGGVVLVDAGDMFQGTLESSLDEGASVVAAYGALG
                     YDAVAIGNHEYDYGPVGPKTTAREPGDDPRGALRARITSAPYAFLSSNLRGDDVHPAL
                     GLPATLIDRAGVQVGIVGVTTEDTPHTTIAANFTGLAIAPLDEAIAAQARSLRARGAA
                     VVVVAAHAGGRCTDLTAPDDLASCDPAQEIMRVARALPPGLVDVIVAGHTHQAMAHRV
                     NGIAILQSYANGVAFGRVDLEVSGRTVVKTTIHPPRRLCSEREASPEVGCVSVDERGA
                     AVMPDPAIAAVITGALTRADVLRNEPLGPKLQADFVVGHRHENPPGNLFTDLMLRARP
                     GVDVAILNGGGLRSTLPAGPLRYGALYQALPFDNRFARVRLRADQLAAMIAVGLGGGP
                     WLSLAGLTVNAECKDARLVVTLLRDGEPLAPDAPLELLTSDFLATGGDKLFAAMGEPG
                     PAAITIEDDPPLREVIAEQLRALGDVELVPATYFEPARPRVKFPGELPLRCAPAG"
     gene            1867..>2095
                     /locus_tag="VGB85_28395"
     CDS             1867..>2095
                     /locus_tag="VGB85_28395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2293 domain-containing protein"
                     /protein_id="HEY0138052.1"
                     /translation="MTESLTLAPTTDPRSFRAPDGTLLSPPDGWDCLPPGDAGMTRRV
                     KLAGPSWAVVEKRGRKLFSRGLWAPAANIAAA"
BASE COUNT          268 a          769 c          788 g          270 t
ORIGIN      
        1 gactttgcgg ccgagctcga ggggtggatc gggcgcccgc cgggggcgtc gttgacggct
       61 tcgctggtcc agcgctgagc ctcgcgtggt agggtcgcgg cgtgtcccga ccccgccgat
      121 cgctggcgct cctgatcctc accgcgtgcg gcccggccga ggcacccgcg ccgcaacccg
      181 agcccgcggc cacgccggtg acgctgacga tcgtcggcac cagcgacctg cacggccacc
      241 tgcgcgcgct gccgctgctg gccgggcacc tcaatggtct gcggcgcgcg cgggcgaacg
      301 acggcggcgg cgtggtgctc gtcgacgcgg gcgacatgtt ccagggcacg ctcgagtcga
      361 gcctcgacga gggcgcgagc gtggtcgcgg cctacggcgc gctcggctac gacgcggtag
      421 cgatcggcaa ccacgagtac gactacgggc cagtggggcc gaagacgacc gcgcgcgagc
      481 cgggcgacga cccgcgcggg gcgctgcggg cccgcatcac ctcggcgccc tacgccttcc
      541 tgtcgagcaa cctgcgcggt gacgacgtgc acccggcgct cggcctgccc gcgacgctga
      601 tcgaccgcgc gggcgtgcag gtcgggatcg tcggggtgac gaccgaggac acgccgcaca
      661 cgacgatcgc ggccaacttc accggcctgg cgatcgcgcc gctcgacgag gcgatcgcgg
      721 cgcaggcccg ctcgctgcgg gctcgcgggg ccgcggtggt cgtggtcgcc gcgcacgcgg
      781 gcggtcgctg caccgacctc accgcgcccg acgacctggc gagctgcgac ccggcgcagg
      841 agatcatgcg ggtcgcccgc gcgctgccgc cgggcctggt cgatgtgatc gtcgccgggc
      901 acacgcatca ggcgatggcg caccgcgtca acggcattgc gatcctgcag tcgtacgcga
      961 acggggtcgc gttcgggcgc gtcgacctcg aggtcagtgg caggacggtc gtgaagacga
     1021 cgatccatcc gccgcgccgg ctgtgcagcg agcgtgaggc gagcccggag gtgggctgcg
     1081 tgtcggtcga cgagcgcggc gcggcggtca tgcccgaccc ggcgatcgcg gccgtgatca
     1141 cgggggcgct gacccgcgcc gacgtattac gcaacgagcc gctcggaccg aagttgcagg
     1201 cggacttcgt cgtcggtcac cggcacgaga acccgcccgg caacctgttc accgacctga
     1261 tgctgcgcgc ccgccccggg gtcgacgtcg cgatcctcaa cggcggaggc ctgcgctcga
     1321 cgctgcccgc cggtccgctg cgctacggcg cgctgtacca ggcgttgccc ttcgacaacc
     1381 gattcgcgcg cgtgcgcctg cgggccgacc agctcgcggc gatgatcgcg gtcgggctcg
     1441 gcggcgggcc gtggctgtcg ctcgccgggc tgacggtcaa cgcggagtgc aaggacgcga
     1501 ggctggtcgt gacgctgctg cgcgacggcg agccgctcgc ccccgacgca ccgctcgagc
     1561 tgctgacgag cgacttcctg gcgaccggcg gcgacaagct gttcgcggcg atgggagagc
     1621 ccgggccggc ggcgatcacg atcgaggacg acccgccgct gcgcgaggtg atcgccgagc
     1681 agctgcgtgc gctcggtgac gtggagctgg tgcccgcgac ctacttcgag ccggcgcggc
     1741 cgcgcgtgaa gttccccggc gagctgccgc tgcgctgcgc gcccgccggt tgacgtcgac
     1801 gactccaacg acatcatcaa ctccatcatg gcgagcgacg atcgagtcgt ggtacctccc
     1861 ccggccgtga ccgagtcgct gaccctcgct ccgaccaccg acccccgcag cttccgggcc
     1921 ccggacggca cgctgctgtc gccgccggac ggctgggact gcctgccgcc gggcgacgcc
     1981 ggcatgacgc ggcgcgtgaa gctggccggg ccgtcgtggg cggtcgtcga gaagcgcggg
     2041 cgcaagctgt tctcgcgcgg cctgtgggcg ccggccgcca acatcgccgc agcac
//
LOCUS       DASWNJ010001709         1745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_700674, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001709 DASWNJ010000000
VERSION     DASWNJ010001709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700674"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VGB85_28400"
     CDS             complement(<1..828)
                     /locus_tag="VGB85_28400"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEY0138053.1"
                     /translation="MSTLPLFDWEPAPLPDLVPEPPAVRPWNRIPAPEGPPELWVIDA
                     TALLVRAYHGMHPRFSPAGLPIGAVLGLSQLLMQVIRRNGSRHVALVYDAGRTTFRNR
                     IDPRYKAHRGEPPEDLAVQLGPALRLGQALGFAAYSVPDYEADDLTATLARLAREAGI
                     GARLVSVDKDLCQLVRDEHPAVRLEDPRSGELCDEAGVRQRMGVAPCRVVDAMALTGD
                     TSDNIPGVRGVGRQAAVALLDAFGDLEGIYADLGRVEALPVRGAKGLARKLEAGREEA
                     "
     gene            893..1072
                     /locus_tag="VGB85_28405"
     CDS             893..1072
                     /locus_tag="VGB85_28405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HEY0138054.1"
                     /translation="MDDSVQVQCPYCFEFVELYIDPEQRGEMIQDCDVCCRPWRVLVR
                     RRGDGSPAVIVDRAQ"
     gene            1100..>1745
                     /locus_tag="VGB85_28410"
     CDS             1100..>1745
                     /locus_tag="VGB85_28410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138055.1"
                     /translation="MSDPGVRKLVLLAAIFFSGCGPKDAPGDEPGSTGASTDTTTGDS
                     TSATPTTSGLPDTTTSTTATTSTMDTTGGPDDICGDFCGRVVECFPGQANAECPCANI
                     DAIGPECVAAWQAARDCYAAESCEGIQQGTEPCWSIVQSALDQCAYGNDGCEVGDVVA
                     DDDSPGSCGIVVDCHETPDRFLVCDGESCTCTREEMTIGTCPADGVCEGNTNPFA"
BASE COUNT          254 a          656 c          597 g          238 t
ORIGIN      
        1 agcctcctcg cgcccggcct cgagcttgcg cgccagtccc ttcgcgccgc gcaccggcag
       61 cgcctcgacg cgcccgaggt cggcgtagat gccctcgagg tcgccgaacg cgtcgagcaa
      121 cgcgaccgcg gcctggcgac cgaccccgcg cacgcccggg atgttgtcgg aggtatcgcc
      181 ggtgagggcc atcgcatcga cgacgcgaca cggcgcgacg cccatgcgct ggcgcacgcc
      241 cgcctcgtca cacagctccc cacttcgggg gtcctcgagc cgcaccgcgg ggtgctcgtc
      301 gcggaccagc tggcacaggt ccttgtcgac gctgaccagg cgcgcgccga tgccggcctc
      361 gcgggcgagc cgggccagcg tcgccgtcag gtcgtcggcc tcgtagtccg gcaccgagta
      421 cgccgcgaag ccgagcgcct gcccgagccg cagcgccggc cccagttgca ccgcgaggtc
      481 ctccggcggc tcgccgcggt gcgccttgta acgcgggtcg atgcggttgc gaaaggtcgt
      541 gcgcccggcg tcgtagacca gcgcgacgtg ccgggagccg tttcgccgga tcacctgcat
      601 caacaattgc gagagcccga gcaccgcccc gatcggcagc ccggccggcg agaagcgggg
      661 gtgcatcccg tgatacgcgc ggaccagcag cgccgtcgcg tcgatcaccc acagctcggg
      721 cggcccctcc ggcgccggga tgcgattcca cgggcgcacc gccggtggct ccggcacgag
      781 gtccggcagc ggcgccggct cccagtcgaa cagcgggagg gtcgacatgc cccaccggtg
      841 tagcgatcgc cggccgccgc ggcaagcccg accgcgcgtg ctatggtcgg ccatggacga
      901 cagcgtgcag gtgcagtgcc cgtactgctt cgaattcgtc gagctctaca tcgaccccga
      961 gcagcgcggc gagatgatcc aggactgcga cgtgtgttgc cgcccctggc gcgtgctcgt
     1021 ccgccgccgc ggcgacggct cccccgccgt catcgtcgac cgcgcccagt gagccgctct
     1081 tatcgtctat gctcgcgcga tgtccgaccc cggggtccgc aagctcgtcc tgctcgcagc
     1141 gatttttttc tccggctgcg gcccgaaaga cgcccccggc gacgagcccg gcagcaccgg
     1201 cgcatccacc gacacgacca cgggtgacag cacctccgcg accccgacga cctcgggtct
     1261 ccccgatacg accacctcga ccaccgcgac cacctcgacc atggacacca ccggcgggcc
     1321 cgacgacatc tgcggggatt tctgcggccg agtagtcgag tgcttcccgg gccaggcgaa
     1381 cgcggagtgc ccgtgcgcga acatcgatgc catcggcccg gagtgtgtgg cggcctggca
     1441 ggcggcccgg gactgttatg ccgcggagtc gtgcgagggc atccagcagg gcacggagcc
     1501 ctgctggagc attgtacagt ccgccctgga ccagtgcgct tacggcaacg acggctgcga
     1561 agtgggggac gtcgtcgccg acgacgactc tcccggctcg tgcggcatcg tggtggactg
     1621 ccacgagacg ccggaccgtt ttctcgtgtg cgacggcgag tcctgcacat gcacgcgcga
     1681 ggagatgacc atcggcacct gccccgccga cggggtctgc gagggcaata cgaacccgtt
     1741 cgcca
//
LOCUS       DASWNJ010001710         2381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_208866, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001710 DASWNJ010000000
VERSION     DASWNJ010001710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208866"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="VGB85_28415"
     CDS             complement(<1..1035)
                     /locus_tag="VGB85_28415"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XylR N-terminal domain-containing protein"
                     /protein_id="HEY0138056.1"
                     /translation="MKVSELDLSIALSFRPQEGKLLLGRERMLVFRQQAFAQLRELLH
                     GFMGTKLAQALLMQFGYHCGRGDYETLSTMYPWDSDADRLGAGPAMHAWEGIVLVEPQ
                     HAEFDRARGHFDFRGLWKNSYEAETYLERFGPAKEPVCYSLCGYGSGWCSAFFGAPVL
                     EIERQCVGAGDPVCAWEIKPIDEWGPVADPWRHALHSTETSVSRELQDKLKIIEAQRT
                     AIAELSSPIIQVWDQVLALPVIGALDDERAANMTARMLDAVQKTRTRHALIDLTGVAQ
                     VDTRSAGHLVTLIRALGLLGCKCYLCGISPTVASTIVTVGIDLREVQTFGTLAAALGT
                     ALGELGVRVER"
     gene            1165..1896
                     /locus_tag="VGB85_28420"
     CDS             1165..1896
                     /locus_tag="VGB85_28420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138057.1"
                     /translation="MSLPSPGSASRPLSLILALAGLLTATGCSAARMELDPQLASAAP
                     EQPVRGLSLAQFRKPVTFGPYSATLTKGGFKSTSQTNAGPFAAKKTSQDFAFTMSGGT
                     SATWAGACSYGSSQRAVLFPINNDAGLVCTLVPEGSAGWQIALASEGKLFGPNSLSGT
                     LTDGQTTYGVKMIHRLANSAFSSAEPVGYELRDAQGVAVAAVQIFNPGQVWIDPRLPA
                     AQQTALAAGAVALLVSRSAASDINE"
     gene            complement(1938..>2381)
                     /locus_tag="VGB85_28425"
     CDS             complement(1938..>2381)
                     /locus_tag="VGB85_28425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138058.1"
                     /translation="ADGGGRGGPGGAGGDGGPGADGGSVTIVVDDRFPELADLVHADV
                     SGGSPGSAGSGGRGGTGGTAPQTCSGCKSLPPGPAGPDGPDGTPGYAAGQNGTMQVKS
                     DDVLSVFAELPPGIRMRPEARPEPVYTPPPPPPTPTKGKRKPKPA"
BASE COUNT          349 a          926 c          760 g          346 t
ORIGIN      
        1 ccgctcgacc ctcaccccga gctcgccgag cgccgtgccg agcgcggcgg ccagcgtccc
       61 gaacgtctgc acttcccgca gatcgatccc gaccgtcacg atcgtgctcg cgaccgtcgg
      121 cgagatccca caaagatagc acttgcaccc gagcagcccc aatgcccgga tcagcgtcac
      181 gaggtgcccg gccgatcgcg tatcgacctg ggcgaccccg gtgaggtcaa ttaatgcgtg
      241 gcgcgtgcgc gtcttctgca ccgcgtcgag catgcgcgcc gtcatattcg cggcccgctc
      301 gtcgtcgagc gcgccgatca ccggcaatgc gagcacttga tcccacactt gaataatagg
      361 cgagctgagc tccgcgatcg ccgtgcgctg ggcctcgata attttgagct tgtcctgcag
      421 ctcgcgcgac acgctggtct cggtcgagtg cagggcgtgg cgccacgggt cggcgaccgg
      481 gccccactcg tcgatcggct tgatctccca cgcgcacacc ggatcgccgg cgccgacgca
      541 ctggcgctcg atctcgagca ccggcgcgcc gaagaacgcg gagcaccagc cgctgccgta
      601 gccgcacagc gaataacaca ccggctcctt cgccggcccg aagcgctcga ggtaggtctc
      661 cgcctcgtag gaattcttcc acagcccgcg aaagtcgaag tgcccgcgcg cgcggtcgaa
      721 ctcggcgtgc tgcggctcga cgagcacgat gccctcccac gcgtgcatcg ccggcccggc
      781 cccgagtcgg tccgcgtcgc tgtcccacgg gtacatcgtc gacagcgtct cgtagtcgcc
      841 gcgcccgcag tgatacccga actgcatcag caacgcctgc gccagcttgg tccccatgaa
      901 tccgtgcagc agctcgcgca gctgggcgaa cgcctgctgg cgaaacacca gcatgcgctc
      961 gcgcccgagc agcagcttgc cctcctgcgg ccggaagctg agcgcgatcg acaggtcgag
     1021 ctcggagacc ttcatgtccc gccagcgtac gctgtccccg agcgcgcgcg aacccgccac
     1081 gtatgcaggc cggcccgctc gtgcccgtga tctgatcgcc accggcccgt cgctaccggc
     1141 agcgatcgtc ccggtcccac ctccatgtcg ctcccatccc ccggatccgc ctcccgcccc
     1201 ctgtccctca ttttggccct cgccggcctc ctgaccgcca ccggctgctc cgccgcccgc
     1261 atggagctcg acccgcagct tgcctccgcc gcgcccgagc agccggtccg cggcctgtcg
     1321 ctggcccagt tccgcaagcc ggtcaccttc gggccgtact ccgccacgct cacgaagggc
     1381 ggcttcaagt cgacctcgca gaccaacgcc gggcccttcg ccgccaagaa gacctcgcag
     1441 gacttcgcct tcacgatgtc cggtggcacc tccgcgacgt gggccggcgc gtgcagctac
     1501 ggcagcagcc agcgcgccgt gctgttcccg atcaacaacg acgccggcct ggtgtgcacc
     1561 ctggtccccg agggcagcgc gggctggcag atcgcgctcg ccagcgaggg caagctgttc
     1621 gggcccaatt cactgtcggg cacgctgacc gacggccaga ccacctacgg cgtcaagatg
     1681 atccaccgcc tcgccaactc ggcgttctcg agcgccgagc cggtcggcta cgagctgcgc
     1741 gacgcgcagg gcgtcgccgt cgcggccgtc cagatcttca acccgggcca ggtgtggatc
     1801 gacccgcgcc tgccggccgc ccagcagacg gccctcgccg ctggcgcggt cgcgctgctg
     1861 gtctcccgca gcgccgccag cgacatcaac gagtaaatgt cggtacatgt cccgcgggac
     1921 atgtaccgac atatcgttca cgccggcttg ggcttgcgct tacccttggt cggggtcggc
     1981 ggcggcggcg gcggcgtgta caccggctcg ggccgcgcct cggggcgcat gcggatgccg
     2041 gggggcagct cggcgaagac cgacaataca tcgtccgact tgacctgcat cgtgccgttc
     2101 tgtccggcgg cgtagccggg cgtgccatcg ggcccgtcgg ggccggcggg accggggggc
     2161 agcgacttgc agccagagca ggtctgcggc gccgtgccac cggtaccacc gcgtccgccg
     2221 gagccggcgg atcccggcga tccgcccgac acgtcggcgt ggaccaggtc cgcgagctcg
     2281 gggaagcggt cgtcgacgac gatcgtcacc gagccgccgt cggcccccgg accaccgtca
     2341 ccaccggcgc cgcccgggcc gccacgaccg ccaccatcgg c
//
LOCUS       DASWNJ010001711         3558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_101764, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001711 DASWNJ010000000
VERSION     DASWNJ010001711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101764"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VGB85_28430"
     CDS             complement(<1..259)
                     /locus_tag="VGB85_28430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138059.1"
                     /translation="MHVSRALWFQLAAMTALSFVWGACKEGTTEVDACGDALPMEGSL
                     CTREGQECLPDEMGCGLYTGVRCSGGVWVFFEAGTGECTGGS"
     gene            complement(338..3328)
                     /locus_tag="VGB85_28435"
     CDS             complement(338..3328)
                     /locus_tag="VGB85_28435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HEY0138060.1"
                     /translation="MFSPDPTRPAPMPQPPIVPPVHPYAEFLGKVAKPGRYIGGEEQQ
                     RAKPTEGLTCRFVLAFPDLYEVGMSHLGTRILYNLINNEADLACERAFSPWIDLEAEL
                     RARGLPLVSLESFTPLRDFDVVGVSLQYELCYTNVLQNLDLGGIPLRSRDRGDRDPIV
                     IAGGPTATHPEPLTPFVDICLVGEAEEVLPGLLRTIGRMRREGAPRAQILAAAAQVPG
                     VYVPAFYYVERDERSELLVVAGRTPEGIAANAPARVARVWVRNLDDYPFPTEFPVPYA
                     EAIFDRAAVEITRGCTEGCRFCQAGIIYRPVRERDPKAIIEAVLKGVDNAGFAETSLT
                     ALSTADVSCIDPLIKELVPELKRRKVKLGVASLRAYGLNDSLLDEIKSVGINGLTFAP
                     EAGTQRMRDVINKNVSDEDILDSARRIFQRGYERIKMYFIMGLPTETEADVVGIAETG
                     RRVREVARELGMARMPSVTVSVSQHVPKPHTPFQWAAMEGLQDLYGKVKLLRDLAKRY
                     RLELKTHNQQESWLECLFARGDRDMADVLENAYKAGARFEGWRECFSFERWLKALADA
                     GVDPNRYTRTLPLDGRLPWDHIDIGLEPGFLAEEYRKALKSRVSPPCGKPFGAKVHAT
                     TPDEAEGDARRLVCYDCGIACDMSEMRQERLVALRGLGEMKGQRHGRERLETDAAEAA
                     GPGAIIPTERLRGRLNPSQHADDSAFKAAAESPFCRVRVFFSKSGPMCFMGHLDLMRV
                     IPRMLRRAGIETAFSRGFTPVPRMSFGPALALGVAAREEVAELDLLLPGAAEDMAGLW
                     SDERRAAEAVALHDRLAAVAPPGLAIVGVRVVAPGERRIGELISGADYSVAIGTEQAA
                     ALASDLPQRLAGPLPIERAPKANKRGLRRASAGDNEVIDVRPRIVAAEVDVAAGILRF
                     RLDMFQEPGEEGAVKGVRPREVVQALCGAPVADHRVCRDRLLAKVEGTYVSLRELAPV
                     FNAMGERWRRDASALAPSEAVDGV"
     gene            3446..>3558
                     /locus_tag="VGB85_28440"
     CDS             3446..>3558
                     /locus_tag="VGB85_28440"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEY0138061.1"
                     /translation="MKDLYTTLGVSKTADAAEIKRAYRKLTQQYHPDKNPG"
BASE COUNT          541 a         1323 c         1188 g          506 t
ORIGIN      
        1 tcgagccgcc ggtgcactcg ccggtgcccg cctcgaagaa cacccagacg ccgccgctgc
       61 agcgcacccc tgtgtacagg ccgcagccca tctcgtcggg caggcactcc tggccctcgc
      121 gggtgcacag gctgccctcc atcggcaggg cgtcgccgca ggcgtcgacc tcggtcgtac
      181 cctccttgca tgcgccccac acgaagctga gggcggtcat cgccgccaat tgaaaccaca
      241 gcgcgcgcga gacgtgcacg ccgcgaggct atcatcggtc gcccggctgt cgagccacgc
      301 gaaggacgat ggccacgcgg ccgcgctact gcgccgatca cacgccgtcg acggcctcgc
      361 tgggggccag cgccgacgca tcgcgccgcc agcgctcgcc catcgcgttg aacacggggg
      421 ccagctccct cagcgagaca tacgtgccct cgaccttcgc aagcaaacga tcacgacaaa
      481 cccgatgatc cgccaccggg gcgccgcaca gcgcttgcac tacttcgcgc ggccggaccc
      541 ccttcaccgc gccctcctcc cctggctcct ggaacatgtc gagccggaac cgcaagatcc
      601 ctgccgcgac atcgacctcg gccgcgacga tccgcgggcg cacgtcgatc acctcgttgt
      661 ctcccgcgct cgcgcgcctc aggccgcgct tgttggcctt gggcgcgcgc tcgatcggca
      721 gcggacccgc gagccgctgc ggcaggtcgc tcgccagcgc cgcggcctgc tccgtcccga
      781 tcgcgaccga gtagtcggcg ccggagatca actcgccgat ccggcgctcg cccggcgcga
      841 ccacgcggac cccgacgatc gccagccccg gcggggcgac ggccgcgagc cggtcgtgca
      901 gcgccaccgc ctcggccgcg cgtcgctcgt cgctccacag ccccgccatg tcctcggcgg
      961 cgccgggcag cagcaggtcg agctccgcga cctcctcgcg ggcggcgacg ccgagcgcca
     1021 gagctggccc gaaggacatg cgcggcaccg gcgtgaagcc gcgcgagaac gcggtctcga
     1081 tgcccgcgcg ccgcagcatc cgcgggatga cgcgcatcag gtcgaggtga cccatgaagc
     1141 acatcggccc cgacttgctg aagaacacgc ggacgcggca aaacggcgac tcggccgcgg
     1201 ccttgaacgc gctgtcgtcg gcgtgctgac tgggattgag gcgcccgcgc aggcgctcgg
     1261 tggggatgat cgccccgggc cccgcggcct cggccgcgtc ggtctcgagg cgctcgcgcc
     1321 cgtgtcgttg gcccttcatc tcaccgagcc cgcgcagcgc gacgagccgc tcctgccgca
     1381 tctcggacat gtcgcaggcg atcccgcagt cgtagcagac cagccggcgc gcgtcgccct
     1441 cggcctcgtc aggggtggtc gcgtgcacct tggcgccgaa gggcttgccg cacggcggcg
     1501 acacccgact cttgagcgcc ttgcggtact cctccgccaa aaagcccggc tcgaggccga
     1561 tgtcgatgtg gtcccacggc agccggccgt cgagcggcag cgtgcgcgtg taacggttcg
     1621 ggtcgacgcc cgcgtcggcc agcgccttga gccagcgctc gaacgagaag cactcgcgcc
     1681 agccctcgaa gcgcgccccc gccttgtacg cgttctcgag cacgtcggcc atgtcgcggt
     1741 caccccgggc gaacaggcac tcgagccagc tctcctgttg gttgtgcgtc ttgagctcga
     1801 ggcggtagcg cttggccagg tcgcgcagca gcttgacctt gccgtacagg tcctgcagac
     1861 cctccatcgc ggcccactgg aacggcgtgt gcggcttggg cacgtgctgc gagaccgaca
     1921 ccgtcacgct cggcatacga gccattccaa gctcacgcgc gacctcgcgg acgcgccgcc
     1981 cggtctcggc gatgccgacg acgtcggcct ccgtctcggt cggcaggccc atgatgaagt
     2041 acatcttgat gcgctcgtag ccgcgttgaa agatccgccg cgccgagtcg aggatgtcct
     2101 cgtcgctgac gttcttgttg atcacgtcgc gcatgcgctg ggtcccggcc tccggcgcga
     2161 aggtcagacc gttgatcccg acgctcttga tctcgtcgag cagtgaatca ttgagcccgt
     2221 acgcgcgcag gctcgcgacc ccgagcttga ccttgcgccg cttgagctcg ggcaccagct
     2281 ctttgatgag cgggtcgatg cacgacacgt ccgcggtgct cagcgccgtc aggctggtct
     2341 ccgcgaagcc ggcgttgtcg acccccttga gcaccgcctc gatgatcgcc ttcgggtcgc
     2401 gctcgcgcac gggccgatag atgatgcccg cctggcagaa gcgacagccc tcggtgcaac
     2461 cacgcgtgat ctcgacggcc gcgcggtcga agatcgcctc cgcgtagggc accgggaact
     2521 cggtcgggaa cggataatcg tcgaggttgc ggacccacac gcgggcgacc cgcgcgggcg
     2581 cgttggcggc gatgccctcg ggcgtgcgcc cggcgaccac cagcagctcc gaccgctcgt
     2641 cgcgctcgac gtaataaaac gccggcacgt acactcctgg cacctgcgcc gccgcggcga
     2701 ggatctgcgc gcgcggggcc ccctcgcggc gcatgcggcc gatcgtgcgc agcagccccg
     2761 gcagcacctc ctcggcctcg ccgacgagac agatgtcgac aaagggcgtc agcggctccg
     2821 ggtgcgtcgc cgtcgggccg cccgcgatca cgatcggatc gcggtcgccg cggtcgcgcg
     2881 agcgcagcgg gatcccgccg aggtcgaggt tctgcagcac gttggtgtaa cagagctcgt
     2941 actgcagact gacgccgacg acgtcgaagt cccgcagcgg cgtgaagctc tcgaggctga
     3001 cgagcggcaa cccgcgcgcg cgcagctcgg cctcgaggtc gatccacggg ctaaaggccc
     3061 gctcgcacgc gaggtcggcc tcgttgttga tcaggttgta gaggatccgc gtgccgaggt
     3121 ggctcatgcc gacctcgtag agatccggga aggcgagcac gaagcggcag gtcagaccct
     3181 cggtcggctt ggcgcgctgc tgctcctcac cgccgatgta gcgccccggc ttggccacct
     3241 tgccgagaaa ctccgcgtag gggtgcacgg gagggacgat cgggggttgc ggcatcggcg
     3301 cggggcgcgt ggggtccggg ctaaacatgg cgttccaggc cggggttttg ccacgaaacc
     3361 cagcaattca ccacccagcg gacccggcgc cgaaacgcga tcgaccactt gccccggccg
     3421 gagggcggcc taacactgtg ccgccgtgaa ggacctctac accacactcg gcgtctccaa
     3481 gacggccgac gctgccgaga tcaaacgcgc ctatcgcaag ctcacgcagc agtatcaccc
     3541 ggacaagaac ccgggcga
//
LOCUS       DASWNJ010001712         1865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_170501, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001712 DASWNJ010000000
VERSION     DASWNJ010001712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170501"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VGB85_28445"
     CDS             <1..699
                     /locus_tag="VGB85_28445"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0138062.1"
                     /translation="SACALGTCGDGGVAPTEACDDGNQENTDTCTSTCTDAACGDGFV
                     QGDEACDAEADNSNNASCTLLCQENVCGDGLVFNATGDAEECDNRVENGPGQACDAMC
                     VANACGDGDLGPDEQCDDGNADPGDGCTASCELEDAGGRRAVRRRQRDAGDGCSATCQ
                     LGRSTRRGGVAGAIRCRVGAPPCRPNQRPTASPGCHARSSGPSCSSDRSAPAAWARSG
                     ARCTATSTRRSRSR"
     gene            639..>1865
                     /locus_tag="VGB85_28450"
     CDS             639..>1865
                     /locus_tag="VGB85_28450"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0138063.1"
                     /translation="MGEVWRAVHRDEHTPVAIKVIGPHWARQPSYRQAFAREVRAIAG
                     LVHPGIVAVHDQGVVGAAAARASGDRIAADSPYLVMEYMPRGSLEGLLGALDWPLVRV
                     LLREILEALAHAHARGIVHCDLKPGNVLLSGTVDVAIKLTDFGVAHALAPGDSLIGDD
                     TPRSAGTPAYMAPEQLLGLWRDFGPWTDLYALGCMLWELCCGTPPFVAGSLVRLAGMH
                     LHEPPGRFTPCFAVPANFEGWLRRLLAKQTRERFACAADAAWALAKLPGPAAPERLSW
                     AAVAAHNAEVDRAAPTQPLPAAIEPPVTDRAAWSVDSTLVGPVVQAAGAGAATADGGA
                     RRTAREGAVEVGGATQRTGDVIDVGGATRSGDAVEVGGATQFKDAVDVGGATRLGDAV
                     DVGGATRLGDAVDVGGA"
BASE COUNT          250 a          630 c          761 g          224 t
ORIGIN      
        1 agcgcctgtg cgctcgggac ctgcggcgac ggcggcgtcg cgccgacgga ggcgtgcgac
       61 gacggcaacc aggagaacac ggatacgtgc accagcacgt gcaccgacgc ggcgtgcggc
      121 gacggcttcg tgcagggcga cgaggcctgc gacgcggagg cggacaactc gaacaacgcg
      181 agctgcacgc tgctgtgcca ggagaacgtg tgcggcgacg gtctggtgtt caacgcgacg
      241 ggcgacgcgg aggagtgcga caaccgggtc gagaacggcc cgggtcaggc gtgcgacgcg
      301 atgtgcgtgg cgaacgcatg cggcgacggg gacctcgggc ccgacgagca gtgcgacgac
      361 ggcaacgccg accccggtga cggctgcacg gcgagctgcg agctcgagga tgcaggcggc
      421 cgtcgagcag tgcgacgacg gcaacgtgac gccggcgacg gctgcagcgc gacctgccag
      481 ctcggacgat ccacccgccg cggtggagtc gcgggtgcga tccgttgtag ggtcggggcg
      541 ccgccatgca ggccgaacca gcgacccaca gcgagcccgg ggtgccacgc gcgcagctcg
      601 ggcccttcgt gctcgagcga ccgatcggcg ccggcggcat gggcgaggtc tggcgcgcgg
      661 tgcaccgcga cgagcacacg ccggtcgcga tcaaggtgat cgggccgcac tgggcccgcc
      721 agccgagcta ccgccaggcg ttcgcgcgcg aggtccgggc gatcgccggg ctcgtgcacc
      781 cggggatcgt cgcggtccac gatcaggggg tcgtcggggc cgcggcggcc cgggcctcgg
      841 gcgatcggat cgcggccgac agcccgtacc tggtgatgga gtacatgccg cgcggcagcc
      901 tcgagggcct gctcggcgcg ctcgactggc cgctggtgcg ggtgttgcta cgcgagatcc
      961 tcgaggccct ggcgcacgcg cacgcgcggg ggatcgtcca ctgcgatctc aagccgggca
     1021 acgtgctgct gtcgggcacg gtcgatgtcg cgatcaagct gaccgacttc ggggtcgccc
     1081 acgcgctcgc gcccggggac tcgctgatcg gcgacgatac cccgcgcagc gcgggcacgc
     1141 cggcgtacat ggccccggag cagctgctcg ggctgtggcg cgacttcggg ccgtggaccg
     1201 acctctacgc gctcggctgc atgctgtggg agctgtgctg cgggacgccg ccgttcgtcg
     1261 ccggcagcct ggtgcgcctc gccggcatgc acctgcatga acctcccggg cggttcacgc
     1321 cgtgcttcgc ggtcccggcc aacttcgagg gctggctgcg ccgcctgctc gccaagcaga
     1381 cgcgcgagcg cttcgcctgc gccgcggacg cggcgtgggc cctggcgaag ctgcccggac
     1441 ccgcggcgcc cgagcgcctg agctgggccg cggtcgcggc ccacaacgcc gaggtcgacc
     1501 gcgcggcacc gacgcagccg ctgcccgcgg cgatcgaacc gccggtgacc gaccgtgcgg
     1561 cgtggagcgt ggactcgacg ctggtcgggc cggtcgtcca ggcggcgggc gccggtgcgg
     1621 cgaccgcaga cggaggcgcg cggcgaacgg cgcgtgaggg cgcggtcgag gtcggcgggg
     1681 cgacgcagcg gacaggcgac gtgatcgatg tcggcggtgc gacgcggtcg ggcgatgcgg
     1741 tcgaggtcgg cggggcgacg cagttcaagg acgcggtcga tgtcggcggt gcgacgcggc
     1801 tcggtgatgc ggtcgatgtc ggcggggcga cgaggctcgg tgatgcggtc gatgtcggcg
     1861 gggcg
//
LOCUS       DASWNJ010001713         1797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_314809, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001713 DASWNJ010000000
VERSION     DASWNJ010001713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314809"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /gene="tssC"
                     /locus_tag="VGB85_28455"
     CDS             <1..1161
                     /gene="tssC"
                     /locus_tag="VGB85_28455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HEY0138064.1"
                     /translation="RENIRLEVVQTTKDELRSDAEDAPDPTQCGLYKHLYSRAFGVLG
                     GKPYGVVCATFDVGASAEDLALMQHCAAVSAMAHVPFLANASPRMFGLASFTELPRIK
                     DLEAAFEGPQYAKWSSFRDHEDARYVGLCAPRFLLRAPYGRRDSELSVKAFDFSEDVV
                     DHHERYLWGPASLAMTARIAESFARYRWCPNIIGPQGGGSVHGLPLHQYEQGGELKTK
                     LPCETAITDRQELQLANLGFIGLVLRQDSDNAAFFSANSSQRPRTFTRTPEGFAAQQS
                     AQLGARLPYMFVITRLAHYLKVLQREQIGTWKGRADLERELNRWIRQYVADMPDPSPE
                     TRSRKPLRRARIVVEDVEDQVGWYRCNLQIEPFIQFEGVSFTLSLVGKLDKQ"
     gene            1169..>1797
                     /locus_tag="VGB85_28460"
     CDS             1169..>1797
                     /locus_tag="VGB85_28460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138065.1"
                     /translation="MLDRRPHPNEEGPTLLFQRNPAAHDKHDKHDKQDERYKRCAGCP
                     FQPVPSDSKPPARHTAAILPVPPLTGPGAPALPGPALSGPQSAVLPRSPAISGPHSAV
                     LARSPGTASAQPAALPKPALLALADARGSGTLVLPDALKPAGPDAATVARPLEPGRPG
                     PTPQRDASPAGHDEMASPDITIMQPTALPLKPAAARPRRAPRAPDARAY"
BASE COUNT          276 a          753 c          531 g          237 t
ORIGIN      
        1 cgcgagaaca tccgcctcga ggtcgtgcaa accaccaagg acgagctacg cagcgacgcc
       61 gaggacgcgc ccgacccgac ccagtgcggc ctctacaagc acctctacag ccgggccttc
      121 ggcgtgctcg gcggcaagcc ctacggggtc gtctgcgcca ccttcgacgt cggcgccagc
      181 gccgaggacc tcgcgctgat gcagcactgc gcggccgtca gcgcgatggc ccacgtaccc
      241 ttcctcgcca acgccagccc gcgcatgttc ggcctcgcca gcttcaccga gctgccgcgg
      301 atcaaggacc tcgaggccgc cttcgagggc ccgcagtacg ccaagtggag cagctttcgc
      361 gatcacgagg acgcgcgcta cgtcggcctg tgcgccccga ggttcctgct gcgcgccccc
      421 tacggccgcc gcgacagcga gctctccgtc aaagccttcg acttcagcga ggacgtcgtc
      481 gaccaccacg agcgctacct gtggggcccc gcctcgctgg cgatgaccgc gaggatcgcc
      541 gagtcgttcg cccgctatcg ctggtgcccc aacatcatcg gcccgcaggg cggcgggtcc
      601 gtgcacggcc tcccgctcca ccagtacgag cagggcggcg agctcaagac caagctgccc
      661 tgcgagaccg cgatcaccga ccgccaggaa ctccagctcg ccaacctcgg cttcatcggc
      721 ctggtactgc ggcaggacag cgacaacgcg gcgttcttct ccgccaactc gtctcagcgc
      781 ccgcgcacct tcacgcgcac gccggagggc ttcgccgccc agcagagcgc ccagctcggc
      841 gcccgcctgc cctacatgtt cgtcatcacc cgcctcgcgc attacctcaa ggtcctgcag
      901 cgcgagcaga tcgggacctg gaagggccgc gcggacctcg agcgcgagct caaccggtgg
      961 atccgccagt acgtcgccga tatgccggac ccctcgccgg agacccgctc gcgcaagccg
     1021 ctccgccgcg cccgcatcgt cgtcgaggac gtcgaggacc aggtcggctg gtaccgctgc
     1081 aacctgcaaa ttgaaccctt cattcaattc gagggcgtct ccttcaccct gtccctcgtc
     1141 ggcaagctcg acaaacagtg aggccccgat gctcgaccgc cgtccccatc ccaacgaaga
     1201 gggcccgacc ctcctgttcc agcgcaaccc cgcggcccat gacaagcatg acaagcatga
     1261 caagcaagac gagcgataca agcgctgcgc gggctgtccc ttccaacctg tcccctcgga
     1321 cagcaaacct cccgcccgcc acaccgctgc gatcctcccg gtcccgccgc tgaccggccc
     1381 gggcgcgccc gcgctgccgg gcccggcgct ttcgggtccg cagtccgcgg tgctcccccg
     1441 ctcgcccgcc atctcgggtc cgcattccgc ggtgcttgct cgctcgccgg gcaccgcgag
     1501 tgcccagccc gccgccctcc cgaaacccgc cctgctggcc ctcgccgacg cccgcggcag
     1561 cggcacgctc gtgctccccg acgccctcaa gcctgcgggc cccgacgcgg cgacggtggc
     1621 ccgcccgctc gaacccgggc gccccggccc gaccccgcag cgcgacgcca gtcccgccgg
     1681 ccacgacgag atggcctcgc ccgacatcac gatcatgcag cccacggcgc tgcccctgaa
     1741 accggccgcc gcgcgtcccc ggcgcgcgcc acgtgctccg gacgcccgcg cctactt
//
LOCUS       DASWNJ010001714         3373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_700741, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001714 DASWNJ010000000
VERSION     DASWNJ010001714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700741"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..2891
                     /locus_tag="VGB85_28465"
     CDS             24..2891
                     /locus_tag="VGB85_28465"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0138066.1"
                     /translation="MAQLTSKAQLLARIGRFIVLEQLGEGGMGVVFAAYDEALDRKVA
                     IKLLRPEGTTTTVHEAMLREAQAMARVSHPNIVTVHEVGALDDQVFIAMEFIRGRTLA
                     AWIPSGPYPWRETVEILLQAGRGLAAAHDAGLVHGDFKPANVMLGDDGAVKVLDFGLA
                     RIHDPHAFADGSAPTEPTLGTPAYMAPELHRGAAASPRSDQYSFCVSLYEALYARDPF
                     ETDESLLAGKIAEPPANVRVPAALLRLVHRGLSVDPARRYPSMTALLAALARTLHRRR
                     RLWLIPLGLAGLFAAALVARSLVPAQDTCAAAPRELQGLWDPAAAETVHARLLATGLA
                     YAEDTWVRVHPRLDAYAAAIVEQRIDACHALDDGRIPEQLFDLRTACLDQRHASLAAL
                     VRILQVGDADVVRNATAAAAALPPVAACNDTQALVDVVTPHGDPTLAARVARVREILA
                     DAQAQELTGQFARGLAIVEAIDLTRVPHPPLLAEIGLRRASLMSELGQHQAADTTLMD
                     ALRHALASRHHLVAATIATRRDFIRAARLQRPGEVLEDGPLVEGMVTRAELYREGAAL
                     RGDHVNNLGIAEAVLGHHAAAQRHFAEALALRREALGADDPQVIYAMGNLGLSYIESS
                     DLDEAVRLLRTALLAAEPTLGPRHPHVALLAINLGYAHRWLAQFDQATVYLARALELQ
                     TEQFGAGSPDLHYVLQVLGDLALDQRHCDEAAAHHGRALALLETRGQGPATADALLGL
                     GRAAACEGDFPGALEHLQRAQAVAIEVHGADDPQVARILDALGDAELAAGRIDQALTH
                     YERAHAIYRIKLGPTAAILAESQRRIGEAQRRRHDLVAATAAAEKALQIYADDGTTDS
                     LASVGVRALLGNLALERGEPAVAYTHFDGAAALLAASADPDNADLALARFGMARALAA
                     QAGETTPAARALAEQALAALRPAANHYPTELAELRAWLVPR"
     gene            complement(2893..>3373)
                     /locus_tag="VGB85_28470"
     CDS             complement(2893..>3373)
                     /locus_tag="VGB85_28470"
                     /inference="COORDINATES: protein motif:HMM:NF015376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HEY0138067.1"
                     /translation="HLAYGTARTVKTIKAALSRYKQSKAGKGGPQVHVGDISARGGGP
                     LKGHMSHQKGIDVDIGLVLKGEDANETRFRTGRADNLDVARTWALLKAFVDSPDVTAV
                     FLDYGLQKLLYEHAKKQKVSESTLDVLFQYPRGRGRYFGIIRHWKGHKDHFHVRFRK"
BASE COUNT          443 a         1450 c         1034 g          446 t
ORIGIN      
        1 atccgctgcc tgctccgccc gcgatggctc agctgacatc caaggcgcag ctgcttgcga
       61 ggatcgggcg ttttatcgtg ctcgagcagc tcggcgaggg cggtatgggg gtcgtcttcg
      121 ccgcctacga cgaggccctc gaccgcaagg tcgcgatcaa gctgctgcgc cccgagggca
      181 cgaccaccac cgtccacgag gcgatgctgc gcgaggccca ggcgatggcc cgcgtctcgc
      241 atcccaatat cgtcaccgtc cacgaggtcg gcgcgctcga cgatcaggtg ttcatcgcaa
      301 tggaattcat ccgcggtcgc accctggccg cgtggatccc ctcgggtcct tacccctggc
      361 gcgagaccgt cgagatcctg ctccaggccg gacgcggcct cgccgcggcg cacgacgccg
      421 gcctcgtgca cggcgacttc aagccggcca acgtgatgct cggcgacgac ggggccgtca
      481 aggtcctcga cttcggcctc gcccgcatcc acgaccccca cgccttcgcc gacggcagcg
      541 ccccgaccga gccgaccctc ggcacccccg cgtacatggc cccggagctg caccgcggcg
      601 ccgcagcctc gccgcgcagc gatcaataca gcttctgcgt cagcctctac gaggcgctct
      661 acgcccgcga ccccttcgag accgacgaaa gtttgctcgc cggcaagatc gccgagccgc
      721 ccgccaacgt ccgcgtgccc gcggcgctgc tgcgcctcgt gcatcgcggc ctctccgtcg
      781 accccgcgcg ccgctacccc tcgatgaccg cgctgctggc cgcgctcgcg cgcaccctgc
      841 atcgccgccg ccgcctgtgg ctgatccccc tcggcctcgc cggcctgttc gccgccgcgc
      901 tcgtcgcccg ctcgctcgtc ccggcccagg acacctgcgc cgccgccccg cgcgagctcc
      961 agggcctgtg ggaccccgcc gcggccgaga ccgtccacgc gcgcctgctc gccaccggcc
     1021 tcgcctacgc cgaggacacc tgggtccgcg tccacccgcg cctcgacgcc tacgccgccg
     1081 cgatcgtcga gcagcgcatc gacgcctgcc acgccctcga cgacggccgc atccccgaac
     1141 agctgttcga cctgcgcacc gcctgcctcg accagcgcca cgccagcctc gccgccctcg
     1201 tccggatcct tcaggtcggc gacgccgacg tcgtccgcaa cgccaccgcc gccgccgccg
     1261 ccttgccccc cgtcgccgcc tgcaacgaca cccaggcgct cgtcgacgtc gtcaccccgc
     1321 acggcgaccc gaccctcgcc gcccgcgtcg ctcgcgtgcg cgagatcctc gccgacgcgc
     1381 aggcgcagga gctgaccggc cagttcgcgc gcggcctggc gatcgtcgag gcgatcgacc
     1441 tgacgagggt cccgcacccg cccctgctcg ccgagatcgg cctgcgccgc gccagcctga
     1501 tgagcgagct cggccagcac caggccgccg acaccaccct catggatgcg ctgcgccacg
     1561 ccctcgccag ccgccaccac ctcgtcgccg ccacgatcgc cacgcgacgc gacttcatcc
     1621 gcgccgcgcg gctccagcga cccggcgagg tgctcgagga cggcccgctc gtcgagggca
     1681 tggtcacccg cgccgagctc taccgcgagg gcgccgccct gcgcggcgac cacgtcaaca
     1741 acctcggcat cgccgaggcc gtgctcggcc accacgccgc cgcccagcgc cacttcgccg
     1801 aggcgctcgc gctgcgccgc gaggccctcg gcgccgacga cccgcaggtc atctacgcga
     1861 tgggcaacct cggcctctcc tacatcgaga gctccgacct cgacgaggcc gtccggctgc
     1921 tgcgcaccgc cctgctcgcc gccgagccga ccctcggccc gcgccacccc cacgtcgccc
     1981 tgctggcgat caacctcggc tacgcgcacc gctggctcgc gcagttcgac caggccaccg
     2041 tctacctcgc ccgcgccctc gagctacaga ccgaacagtt cggcgccggc tcgcccgacc
     2101 tgcattacgt cctccaggtc ctcggcgacc tcgcgctcga ccagcgtcac tgcgacgagg
     2161 ccgcggccca ccacggccgc gccctcgcgc tgctcgagac ccggggccag gggcccgcca
     2221 ccgccgacgc gctgctcggc ctcggccgcg ccgcggcctg cgagggcgac ttccccggcg
     2281 cgctcgagca cctgcagcgg gcccaggcgg tcgcgatcga ggtccacggc gccgacgacc
     2341 cgcaggtcgc ccggatcctc gacgccctcg gtgacgccga gctcgccgcc ggccgcatcg
     2401 accaggcgct gacccactac gagcgcgccc acgcgatcta ccgcatcaag ctcggcccga
     2461 ccgccgccat cctcgccgaa tcacagcgaa ggatcggcga ggcgcagcgc cgccggcacg
     2521 acctcgtcgc cgccaccgcc gctgccgaga aggccctcca gatctacgcc gacgacggga
     2581 ccaccgatag cctcgccagc gtcggcgtgc gcgcgctgct cggcaacctc gcgctcgagc
     2641 gcggcgagcc ggccgtcgcg tacacccact tcgacggcgc cgccgccctg ctcgccgcct
     2701 ccgccgaccc cgacaacgcc gacctcgcgc tcgcccgctt cggcatggcc cgggccctcg
     2761 ccgcccaggc cggcgaaacg accccggccg cccgcgccct cgccgagcag gccctcgccg
     2821 ccctccggcc ggccgccaac cactacccca ccgagctcgc cgagctgcgc gcctggctcg
     2881 tcccccgctg actcacttgc gaaagcggac gtggaagtgg tccttgtggc ccttccagtg
     2941 acgaatgatg ccgaagtagc gcccgcgtcc gcgcgggtac tggaacagca cgtcgagcgt
     3001 gctctcgctg accttttgct tcttcgcgtg ctcgtacaac agcttttgca gcccgtagtc
     3061 gaggaacacc gcggtgacgt ccgggctgtc gacgaacgcc ttgagcagcg cccaggtgcg
     3121 cgcgacgtcg aggttgtcgg cgcgcccggt gcgaaaccgg gtctcgttgg cgtcctcgcc
     3181 cttgagcacc agaccgatgt cgacgtcgat gcccttttgg tgcgacatat ggcccttgag
     3241 cggcccaccg ccgcgggcgc tgatgtcccc gacgtgcacc tgcggcccgc ccttgccggc
     3301 cttgctctgc ttgtaccgcg acagcgccgc cttgatcgtc ttgaccgtgc gggctgtccc
     3361 ataagccagg tgc
//
LOCUS       DASWNJ010001715         5700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_596180, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001715 DASWNJ010000000
VERSION     DASWNJ010001715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596180"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..953)
                     /locus_tag="VGB85_28475"
     CDS             complement(111..953)
                     /locus_tag="VGB85_28475"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEY0138068.1"
                     /translation="MPTTLAAEMLAAARRLCQEVDALRFGPPVTHVYNPLAYAWAAQA
                     QYIERHASTKKRVVLLGMNPGPFGMGQTGVPFGEVARVRGFLGIEATVNKPAHEHPAR
                     PVQGFACTRSEVSGARVWGLVESRWGTAQAFFVDHYVANYCPLLFMEASGKNRTPDKL
                     ASAERAALTAACDRHLLRTIDLLAPEWVIGIGGYAEQQARTALGDRVKIGRILHPSPA
                     SPAANLGWADAAVAELRALGIAVPDPKDMSGMAAAGATPPKPARARAPRQTRVAGGTR
                     VHGG"
     gene            1194..3395
                     /locus_tag="VGB85_28480"
     CDS             1194..3395
                     /locus_tag="VGB85_28480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0138069.1"
                     /translation="MRWTPVAPLAASLLLGCGLDLGGGGSCGNASCSASLDLDTGSSE
                     TAGDTDADTDTGGPLNFWPGVMCTPDADETPRFYFDLRAGKDPERDYFRLPFPADLRL
                     KDGGLDLEGFPRAPARFAPAPELAAVIDRWMAHIEQDMPGFAVDGAILFRTNTGVADV
                     RGIHYINVTPDHPEYGEAIQGLTFNAKTGASSRTNYLCENWLAIEPIDGVVLDPGATY
                     AVVITDASVPVGGGKFVPDADLRAMLSQSPPQDPLQAQAWPTFAPLRTFLSSPANTQD
                     LRSDQLVAATVFTAAPHRDLMARAREAVHAGPLHVSDLHVCEAAGPSPCSTSPGLTDA
                     EREQRRCGAPNKHYTEIQGRVTLPIFQEGRAPYASNGGKIQVDGDGPVLHAVQDVCFA
                     LTVPKGEVPGAGWPTLVVAHGTNGSFRSAIGDGTAGHVASAGIATLALEGTVHGERRG
                     DTDSDGLVEGLPIDQLVFNLRNPDAARDNTLQGAIDQFTAVRLARQLAEATAPEPAPA
                     VLDPNNVYFMGHSQGAQAGAVFLPYEPDVHAAVLSGGGANLLRALLAKTEPKVTLGPV
                     AYPPKDLLQLAFQERPDQPLTSAHPLLMLFNNFVNRSDADVYSPLLIDKHVLMYMGHV
                     DHYTPLRTAGSLAIGARLEVGGKNMFPAPCDQYEADDERTACNYTASGFLRETPLPAS
                     GNRGGVTGVVLMRDEANGLDGHYVAFTAAEQARIVDYLVSARDKAVPVVGD"
     gene            3564..4004
                     /locus_tag="VGB85_28485"
     CDS             3564..4004
                     /locus_tag="VGB85_28485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HEY0138070.1"
                     /translation="MHRTYGTATCIAAGTKVTTADGQIPIDMIEILDELLTLDPERGL
                     LIPARVIAVRRAERECVCLHLPGGTLRLTSDHPVWSPETCRYEAAGLWVTGHLQLLTC
                     LAGDRAGLTVVTARESFVGVLPVLDLVLAAPQHSFLASQVLVHS"
     gene            4070..4237
                     /locus_tag="VGB85_28490"
     CDS             4070..4237
                     /locus_tag="VGB85_28490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138071.1"
                     /translation="MRSVSLILAFGFVVVLGLLVVLVLSGAAEKQAVACFSMSFLGIG
                     IAIGLGLSERR"
     gene            complement(4301..>5700)
                     /locus_tag="VGB85_28495"
     CDS             complement(4301..>5700)
                     /locus_tag="VGB85_28495"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0138072.1"
                     /translation="CTDQCTTPTCGDSFVQASLGEACDLGGQNSDAGACTLGCKTAVC
                     GDALVQAGVEQCDAGANNGNSKACKANCTDNVCGDGFVEVGVEDCDDANAVDNDACSN
                     VCKAASCNDGIKNGQEVDVDCGGPTCGLCPTVILLAGGNTGPNGHVGATFTNALGWST
                     TPLAGVTVTDLDLTMTSANVGVGVLRYTKINDPLDNRLQYTVWNNGVWTPVNQVSNFT
                     TFHAPAIDSADTTAQVVFRGTDANYYYTAFVNNAWTAAQATGASGPIAGDIAALGANA
                     AYFYANQATNRMNSVDRQNAVWTAPLTPYGIYHKTIQPNLIRMTAGAAELMAVWPYIN
                     PDEKKVRFGVRVNGVWQANQDVPSVLTTYRVGTAPLPLGRVALVYHGMDDKLYASLFT
                     GGQWSPSFGLPGEPTITGTPAVVQGIGIYEAEVAYISAGQVWHTRFDADTFTWKAPVV
                     VGGANMQSVALARSK"
BASE COUNT          794 a         2231 c         1809 g          866 t
ORIGIN      
        1 ttgtcgccgt tgggggtcgt ccacgagtac aggtcgagca tggtcgggcg agcatagccc
       61 cgtccccacg gcggctgaca gcgtggaccg gccccacgcc caaatgaccc ctatcccccg
      121 tgaacccgcg ttccgccggc cacgcgcgtc tgccgcggcg cccgtgcgcg cgccggcttc
      181 ggcggcgtcg ccccggcggc ggccattccg gacatgtcct tcgggtcagg caccgcgatc
      241 ccgagcgcgc gcagctcggc gaccgccgcg tccgcccacc cgaggttcgc cgccgggctc
      301 gccgggctcg ggtgcaagat ccgcccgatc ttgacgcgat cgccgagcgc cgtgcgggcc
      361 tgctgctcgg catatccgcc gatcccgatc acccactccg gcgccagcag atcgatcgtc
      421 cgcagcaggt gccggtcgca cgcggccgtc agcgccgctc gctcggcgct cgccagcttg
      481 tccggcgtcc ggttcttgcc gctcgcctcc atgaacagca acggacaata attggcgacg
      541 tagtggtcga caaagaacgc ctgcgccgtg ccccagcgcg actcgaccag cccccacacc
      601 cgcgcaccac tgacctcgct gcgcgtgcac gcgaaaccct gcaccggccg cgccgggtgc
      661 tcgtgcgccg gcttgttcac cgtcgcctcg atgccgagga acccacgcac gcgcgccacc
      721 tcgccgaacg gcacccccgt ctggcccatc ccgaacggcc ccgggttcat gccgagcagc
      781 acgacccgct tcttcgtgct cgcgtgacgc tcgatgtact gcgcctgcgc ggcccaggcg
      841 taggccagcg ggttgtacac gtgggtcacc ggcggcccga agcgcagcgc gtcgacctcc
      901 tggcacaggc ggcgggcggc ggccagcatt tcggcggcga gggtcgtcgg catcgtggct
      961 gccggcatag tccgcccgcc cgcgccacgc aagcgccgcg accccccgca gccgtgcagg
     1021 tcccgggtct ggccgcgtcg gcccggaccg ggccccgcgc cgcatacacc ggcgcacacg
     1081 tcgccttcgt tgcacttgca tcagtcgggc gaccacgccg cccggggccc gccgatggat
     1141 cttcatgcgg atacaagtgt tctgctacgc gacacgctgc tatgtttcgc ggcatgcgct
     1201 ggaccccggt cgcccccctc gccgcgtccc tcctcctcgg ctgtggtctc gacctcggcg
     1261 gcggcggctc ctgtggaaac gcgagctgct ccgcttcact cgacctcgac accggcagca
     1321 gcgagaccgc cggcgacacc gacgccgaca ccgacacggg aggcccgctc aacttttggc
     1381 cgggcgtcat gtgtacgccg gacgcggacg agaccccgcg gttttacttc gacctgcgcg
     1441 ccggcaagga ccccgagcgc gactacttcc gcctgccgtt tccggccgac ctgcgcctca
     1501 aggatggcgg cctcgacctc gagggtttcc cgcgcgcgcc ggcccgcttc gcgcccgcgc
     1561 cggagctcgc cgccgtcatc gaccgctgga tggcgcacat cgagcaggac atgcccggct
     1621 tcgccgtcga cggcgcgatc ctctttcgga ccaacaccgg cgtcgccgac gtccggggga
     1681 tccactacat caacgtcacg cccgatcacc ccgagtacgg cgaggcgatc cagggcctga
     1741 ccttcaacgc caagaccggc gccagcagcc gcaccaacta tctctgcgag aactggctgg
     1801 cgatcgagcc gatcgacggc gtcgtgctcg accccggggc cacctacgcg gtcgtgatca
     1861 ccgacgcgag cgtgcccgtc ggcggcggca agttcgtccc cgacgccgac ctgcgggcca
     1921 tgctgtccca gtcgcccccg caggacccgc tgcaggccca ggcgtggccg accttcgccc
     1981 cgctgcgcac cttcctcagc agcccggcga acacccagga cttgcggtcc gaccagctcg
     2041 tcgccgccac cgtgttcacc gcggcgccac atcgtgacct gatggcccgg gcccgcgagg
     2101 ccgtgcacgc cggtccgctg cacgtctcgg acctgcacgt ctgcgaggcc gccggcccct
     2161 cgccgtgttc gacctctccc ggcctcacgg acgccgaacg cgagcagcgc aggtgcggcg
     2221 cgccgaacaa gcactacacc gagatccagg gccgcgtcac gctcccgatc ttccaggagg
     2281 gtcgtgcgcc gtacgccagc aatggcggca agatccaggt cgacggcgac gggcccgtct
     2341 tgcatgccgt gcaggacgtc tgcttcgcgc tgactgtccc caagggcgag gtccccgggg
     2401 ccggctggcc gaccctcgtg gtcgcccacg gcaccaacgg cagcttccgc agcgcgatcg
     2461 gtgacggcac cgccggtcac gtcgccagcg ccggcatcgc cacgctcgcg ctcgagggca
     2521 ccgtgcacgg cgagcgccgc ggcgacaccg acagcgacgg cctcgtcgag ggtctgccga
     2581 tcgatcagct ggtgttcaac ctgcgcaacc cggacgccgc ccgcgacaac accttgcagg
     2641 gcgcgatcga tcagttcacc gccgtgcgcc tcgcccggca gctcgccgag gccaccgcgc
     2701 cggagcccgc ccccgccgtg ctcgacccca acaacgtcta cttcatgggc catagccagg
     2761 gcgcgcaggc cggcgccgtg ttcctgccct acgagcccga cgtccacgcc gccgtgctca
     2821 gcggcggcgg tgccaacctg ctccgcgcct tgctggcgaa gaccgagccc aaggtcacgc
     2881 tcggccccgt cgcctatccc ccgaaggacc tgctgcagct cgccttccag gagcgccccg
     2941 accagccgct gaccagcgcg cacccgctgc tgatgctctt caacaacttc gtcaaccggt
     3001 ccgacgccga cgtgtacagc ccgctgctga tcgacaagca cgtgttgatg tacatgggcc
     3061 acgtcgatca ctacaccccg ctgcgcaccg ccggcagcct ggcgatcggc gcgcgcctcg
     3121 aggtcggcgg caagaacatg ttccccgccc cctgcgatca atacgaggcc gatgacgagc
     3181 gcaccgcctg caactacacc gccagcggct tcctgcgcga gacccccctg cccgcctccg
     3241 gcaaccgcgg cggcgtcacc ggcgtcgtct tgatgcgcga cgaggcgaac ggcctcgacg
     3301 gccactacgt cgccttcacc gccgccgagc aggcgcgcat cgtcgattac ctcgtctccg
     3361 cgcgcgacaa ggccgtcccg gtcgtcggcg actgaccgga cccgaaagcg ggcgcccgcg
     3421 cgccgttcgc catatgcgct gccgcgggtg catgagaccc cgcggcagca ccatgttccg
     3481 tggcccacgg cacccccatc gcgcggctgg gcgcgaaagc tcgccttgaa ggccgggcat
     3541 cgcgggccaa tgctcgcccg gtcatgcacc gcacatacgg tacggccacc tgcatcgccg
     3601 cgggcacgaa ggtgaccacc gccgacggcc agatcccgat cgacatgatc gagatcctcg
     3661 acgagctcct caccctcgac cccgagcgcg gcctgctgat ccccgcccgc gtcatcgccg
     3721 tgcgccgcgc cgagcgcgag tgtgtgtgcc tgcatctccc cggcggcacc ctgcgcctga
     3781 ccagcgacca ccccgtctgg tcgcccgaga cctgccgcta cgaggccgca ggtctctggg
     3841 tcaccgggca cctccagctg ctcacctgcc tcgccggcga cagggccggc ctcaccgtcg
     3901 tcaccgcccg cgagagcttc gtcggcgtgc tgccggtcct cgacctggtg ctcgccgcgc
     3961 cgcagcacag cttcctcgcc agccaggtgc tcgtccacag ctagccgcgg gatcgcgcgc
     4021 gccaaagttg ccggctcgtt ttgcaggacc ctgtggttag actgccgcgg tgcgcagcgt
     4081 atccctgatc ctcgccttcg gcttcgtcgt cgtcctcggc ctgctcgtcg tgctcgtgct
     4141 cagcggagcc gccgagaagc aagccgtcgc ctgcttctcg atgtcttttc tgggaatcgg
     4201 catcgccatc ggcctcggcc tcagcgaacg ccgctaaacg gccgaacgac ggctgtcatg
     4261 agcggcgagc cccggcgagc gccgggaccc gcccgcgtaa tcacttgctg cgcgcgagcg
     4321 cgacgctctg catgttggcc ccgccgacca cgacgggcgc cttccacgtg aacgtgtcgg
     4381 cgtcgaagcg ggtgtgccac acctgcccgg cgctgatgta cgcgacctcg gcctcgtaga
     4441 tgccgatgcc ctgcacgacc gccggcgtcc cggtgatcgt cggttcgccg ggcaggccga
     4501 agctcggcga ccactggccg ccggtgaaca gcgatgcata cagcttgtcg tccatgccgt
     4561 gatacacgag ggcgacccgc ccgaggggca gcggcgccgt gccgacgcgg tacgtggtca
     4621 gcacactggg cacgtcctga ttggcctgcc acaccccgtt gacccgcacc ccgaaccgca
     4681 ccttcttctc gtcggggttg atgtacggcc acaccgccat cagctcggcc gcgcccgccg
     4741 tcatccggat caggttcggc tggatcgtct tgtgatagat gccgtacggg gtcagcggcg
     4801 ccgtccagac cgcgttctgg cggtccacgc tgttcatccg gttggtcgcc tggttggcat
     4861 aaaaatacgc cgcgttggcc ccgagcgccg cgatgtcgcc cgcgatcggt ccggaggcgc
     4921 cggtcgcctg cgcggccgtc caggcgttgt tgacgaacgc cgtatagtag tagttcgcgt
     4981 ccgtcccgcg gaacaccacc tgcgcggtgg tatcggcgga gtcgatcgcc ggcgcgtgga
     5041 aggtcgtgaa gttgctgacc tgattgaccg gcgtccacac cccattgttc caaaccgtgt
     5101 attgcaggcg gttgtcgagc gggtcgttga tcttcgtgta acgcaacact cccacgccga
     5161 cgttcgccga ggtcatcgtc aggtcgaggt ccgtgaccgt cacgccggcc agcggcgtgg
     5221 tcgaccagcc gagcgcattg gtaaacgtcg cgccgacgtg cccgttgggc ccggtgttgc
     5281 cgcccgcgag caggatcacc gtcgggcaca ggccgcaggt cggcccgccg cagtcgacgt
     5341 cgacctcctg gccgttcttg atcccgtcgt tgcacgaggc cgccttgcac acgttgctgc
     5401 acgcgtcgtt gtcgaccgcg ttggcgtcgt cgcagtcctc gaccccgacc tccacaaatc
     5461 cgtcgccgca cacgttatcg gtgcagttgg ccttgcacgc cttgctgttg ccgttgttcg
     5521 cgcccgcgtc gcactgctcg accccggcct gcacgagcgc gtcgccgcac accgccgtct
     5581 tgcacccgag cgtgcacgcg ccggcgtcgc tgttctgccc gccgaggtcg caggcctcgc
     5641 cgaggctcgc ctgcacgaag ctgtcgccgc aggtcggcgt cgtgcactgg tcggtgcagc
//
LOCUS       DASWNJ010001716         1601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_562961, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001716 DASWNJ010000000
VERSION     DASWNJ010001716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562961"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /locus_tag="VGB85_28500"
     CDS             <1..1372
                     /locus_tag="VGB85_28500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY0138073.1"
                     /translation="RPGQPAAPRRGTGAPAGRPMTGPQPIRSRDPGLPTAPPPPTLSV
                     PPPTPRQDVPVTPPRPIQGVPPARPVPADSPARPVPSDSPRIRVPEPGGYREAASNAG
                     DVIELALQAHRDGASDLLLSTGLPARLRLGGTLRETGLVCDAEQILALAALGWSAERR
                     LEFERSGSVDMALDLSPGPGARPLRFRVNMFRQQRGLAASIRPVRAEVPSLAQLGLPE
                     DFKALAQYPSGLVLMTGPTGSGKSTTLASLIEHVNQTSAKHVLTLEDPVELQYTSKRS
                     IIHQREVGTDVESFASGLRAALREDPDIILVGEMRDQDTIAAALTAAETGHLVLSTLH
                     SASAGMAIDRIIDVFPGPKQPQVRLQLAGALRAVITQVLLPSARGLDRVPAYEKMIVT
                     AAVASQIREGRAHQIANQIQTGRAEGMQSLEQSLLALLRAGKINIEAAMDVAPDPDSL
                     RRAYRG"
BASE COUNT          199 a          581 c          607 g          214 t
ORIGIN      
        1 gcgcccgggg cagccggcgg ccccgcggcg cggcaccggg gcgcccgctg gccgaccgat
       61 gaccggcccg cagccgatcc gcagccgtga ccccgggctg ccgaccgccc cgccgccgcc
      121 gacgctatcg gtgccaccac cgaccccgcg ccaggacgtg cccgtcacgc cgccgcggcc
      181 gatccagggc gtgcctccgg cgcgacctgt cccggcggac agcccggcgc gacctgtccc
      241 gtcggacagc ccgcggatcc gcgtcccgga gcccggcggc taccgcgagg cggcgtcgaa
      301 cgccggtgat gtcatcgagc tcgcgttgca ggcccaccgc gacggcgcgt cggacctgct
      361 gctgtcgacc ggcctgccgg cgcgcctgcg cctcggcggc acgctgcgcg agacgggcct
      421 ggtctgcgac gccgagcaga tcctcgcgct cgcggcgctg ggctggagcg ccgagcgacg
      481 tctcgagttc gagcgcagcg gcagcgtcga catggcgctc gacctgtccc cggggccagg
      541 tgcccgcccg ctgcgctttc gcgtcaacat gtttcgccag cagcgcggcc tcgcggcgtc
      601 gatccggccg gtgcgcgcgg aggtgccgtc gctggcgcag ctcgggctgc cggaggactt
      661 caaggcgctc gcgcagtacc cctcgggcct ggtgttgatg accggcccga ccggctcggg
      721 caagtcgacc acgctcgcct cgctgatcga gcacgtcaat caaacctcgg ccaagcacgt
      781 gctgacgctc gaggacccgg tcgagctgca gtacaccagc aagcggtcga tcattcacca
      841 gcgcgaggtc ggcaccgacg tcgagagctt cgcctcgggc ctgcgcgcgg cgctgcgcga
      901 ggacccggac atcatcctcg tcggcgagat gcgcgaccag gacacgatcg cggcggccct
      961 gaccgcggcc gagaccggcc acctggtgct ctcgaccctg cacagcgcca gcgccggcat
     1021 ggcgatcgac cgcatcatcg acgtgttccc ggggcccaag cagccgcagg tccgcctgca
     1081 gctcgcgggg gccctgcggg cggtcatcac ccaggtgctg ctgccgtccg cccgcgggct
     1141 cgaccgggtg ccggcgtatg aaaagatgat cgtgaccgcg gcggtggcct cgcaaattcg
     1201 cgagggccgc gcgcatcaga tcgccaacca gatccagacc ggacgcgccg agggcatgca
     1261 gtcgctcgag cagtcgctgc tcgcgttgct gcgcgccggg aagatcaaca tcgaggcggc
     1321 gatggatgtc gctcccgacc cggacagctt gcggcgggcg tatcgagggt aagggcatgg
     1381 ccagtcgatc gcgagggggg cgggcccggc cgggcgtgcg tggggactgg ctgcttgcgc
     1441 ggctgtttgc gggtgtgctc gggctatgtt gggcgcggct tgcgggcgtg ttcgggcaat
     1501 gcggtgcgcg gctgcctgtg ggcgtgttcg gagagcgcgg ggctcggctg tgtgggccgg
     1561 ggggcggggg ccgacgggct gcgggtccct gcgacgcggg t
//
LOCUS       DASWNJ010001717         3745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_524671, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001717 DASWNJ010000000
VERSION     DASWNJ010001717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524671"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(439..642)
                     /locus_tag="VGB85_28505"
     CDS             complement(439..642)
                     /locus_tag="VGB85_28505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HEY0138074.1"
                     /translation="MSKIGDRFKTGQTCDTSGIYGFDGYTDASAGPQPTQEERVITLR
                     ASQTFPPIRSTNHGAYWKLQRAT"
     gene            1010..1246
                     /locus_tag="VGB85_28510"
     CDS             1010..1246
                     /locus_tag="VGB85_28510"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HEY0138075.1"
                     /translation="MRATSGPVARPNTITTPIDSDGDRVQDAADKCPQQPGSLRDGCP
                     VVDTDKDGISDPYDKCPTVPEKRDGLADGDGCPL"
     gene            1270..2946
                     /locus_tag="VGB85_28515"
     CDS             1270..2946
                     /locus_tag="VGB85_28515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138076.1"
                     /translation="MHRPHVLVALLLTACGPSAMATSETTATSTSQVDSETGTTEATP
                     TTGDAPDPCECADPQVVEGDLDVPGLDGFDGFCIGEVTGRLTIQDVMDPAMLGPLAHL
                     RRAGDLWIRGNPGLVDLSALGCLEQVHGALDVHDNPALVDLDGLARLQTARRVTLGRL
                     PLTALPGFAPGFHGVEAIVLEALPGIVDLDPLAGWSSALGELEVFVSIYDLPALASIA
                     ALAGPLGPPGTPNAVASIELSRLPALTAVTGLEAVTRGNVRLYGLPKVESLAPLAALE
                     QVESLQLIGMGLTSLQGLHNLESAQDVQIGGCQPSDRLDGIVDLTGLDALTQVGGTLA
                     ILGNPNLVALSGAPMLSTAARIDIVDNPKLGADAVAAFEAQVTHKHLCAGDMVDCGCL
                     GAYPDAIDEGCADAWSGGSAVVASGKAGALDGTTAFFGYRSFGNDRWLTLVVLDTAAD
                     IATAIDDGTYYTSDAGAPKLVFETQRLYDDWIGVSTEYAELSQPGGSQTSFQVQVTVT
                     GRLGNWLMTDPTDPPRLIGEFATIDPNAATSVQGSFDAAFCEDFVSQIND"
     gene            complement(2950..>3745)
                     /locus_tag="VGB85_28520"
     CDS             complement(2950..>3745)
                     /locus_tag="VGB85_28520"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEY0138077.1"
                     /translation="GDRAFAVVDDETGRVASAKHPRLWGALLGYRARFVEPLADAQRH
                     APVEITAPDGTTARSDGDAQRLLSAALGRAVRLSATPGPQAKYDQAEPGLDTARGEAL
                     AVGAGAGTFFDFAPVHLVSTASLARLREVQPASDFAVARFRPNLVIDTGAAQGFVEND
                     WLGQVLSIGAQVQLCVTFPCPRCVMTTLAQGPLAADPGVLRAAAEHNMRMFALLARRM
                     PTVGVYATVVRGGTIASGDAVRLEGHAPLRRLAAVAGAVKRAVRRR"
BASE COUNT          554 a         1391 c         1227 g          573 t
ORIGIN      
        1 cacgacgaaa aaaacttccg cgtcgccgtc gcggacgccg acaacacgcg agacacccga
       61 aacgatgtga cggggtttcg ctgcgccgcg aaggtccaca gcgagacccc aaagcgatga
      121 agcctccgac tggcgcggac agcgagggct tcgctgtcct cgtcctcatc tgcgaccgta
      181 ctggtcgcgg atatagcggt tgaagtagct tcccggcgag gcagcggcgc agagaccctc
      241 atagtgatgc cgaggaaccc cgaagtaatc gtatgtgcct ctattggagc cgaaccagat
      301 ccggagcgtc cccgcggcgt cgtcgtactc ggcccagaca atggcggtcg aattcaagta
      361 cggcatggct tctcgtctgc ggctcacggg gtaggcgcac gagggccgcc atgcggcccc
      421 gtgcgccttg atggtgggtc aggtcgcgcg ctgtagcttc cagtacgcgc catggttggt
      481 cgaccggatg ggcggaaagg tctgcgaggc gcgcagagtg atcacccgct cctcctgggt
      541 cggctgcggc ccggcggacg cgtccgtata gccgtcaaac ccgtagatac cgctcgtgtc
      601 gcaggtctgg ccagtcttga aacgatctcc gattttgctc actgtgttct ccttggacga
      661 tcggtgcgtg ctgcaccgcg ctcactgatg tgcctgtgac ctgccgcggt gatcacgaga
      721 gtgacttttt attcggccgc gcaaacggcg ccaactgggc tcgatttctc ggtcgatgtc
      781 gatagccgtt tggcacccct cgtttgcggc cagaggtctc ccgaacaacg ccagctgcct
      841 cgcccatggc cgccgcgccg ctcgtcgcac attttcccca ctttcgcgtg cgcgcggatc
      901 cagctgagct gttatgctcg tgatcaccat gggtcgcgca cgcctcttgt tctccgcctt
      961 gctgctcctc ggatgcgcac agacgccccc tccccccgcg cccgtgccga tgcgcgcgac
     1021 gagcgggccg gtcgcgcgtc cgaacacgat cacgacgccg atcgacagcg acggtgatcg
     1081 tgtccaggac gccgcggaca agtgccctca gcagccaggg tcccttcgtg acggctgccc
     1141 ggtcgtcgac accgacaagg acggcatctc cgacccctat gataagtgcc cgaccgtgcc
     1201 cgagaagcgc gacggcctcg ccgacggcga cggctgccct ttgtgacgcc gcggtcgtat
     1261 gcttcggcga tgcatcgccc gcacgtcctc gtcgccctcc tgctgaccgc ctgcgggccg
     1321 agcgcgatgg cgacgagcga gacgacggcg acctcgacct cgcaggtgga cagtgaaacc
     1381 ggcacgacgg aggcgacgcc gaccaccggc gatgcgccgg acccgtgcga atgcgccgac
     1441 ccgcaggtgg tcgagggtga cctcgacgtc cccggcctcg acggcttcga cggcttctgc
     1501 atcggcgagg tcaccggccg cctgacgatc caggacgtga tggaccccgc gatgctgggg
     1561 ccgctggccc acctgcggcg cgccggtgac ctgtggatcc gcggcaaccc ggggctcgtc
     1621 gatctctcgg ccctcggctg cctcgagcag gtccatggcg cgctcgatgt ccacgacaac
     1681 cccgcgctcg tcgacctcga cggcctcgcc cgcctgcaga ccgcccgccg cgtcaccctc
     1741 gggcgcctgc cgctcaccgc cctgccaggc ttcgcgcccg gctttcacgg cgtcgaggcg
     1801 atcgtgctcg aggctctgcc ggggatcgtc gacctcgacc cgctcgccgg gtggtcgagc
     1861 gccctcggcg agctcgaggt cttcgtgtcg atctacgacc tgcccgcgct cgccagcatc
     1921 gccgcgctcg ccgggccgct cggacccccg gggacgccga acgcggtcgc gtcgatcgag
     1981 ctctccaggc tccctgcgct gaccgccgtg accggcctcg aggcggtcac gcgcggcaac
     2041 gtgcgcctct acgggctgcc gaaggtcgag tcgctcgccc cgctcgccgc gctcgagcag
     2101 gtcgagagcc tgcaattgat cgggatgggc ctgacctcat tgcagggcct ccataacctc
     2161 gagtccgcac aggacgtgca gatcggcggg tgccagccct ccgaccgcct cgacgggatc
     2221 gtcgacctca ccggcctcga cgcattgacg caggtcggcg gcacgctggc gatcctcggt
     2281 aatccgaacc tcgtcgcgct gtccggggcg ccgatgctgt cgaccgccgc gcgtatcgac
     2341 atcgtcgata atcccaagct cggcgccgac gcggtcgcgg ccttcgaggc ccaggtcacg
     2401 cacaaacacc tgtgcgcggg cgacatggtc gattgcggct gcctcggcgc gtacccggat
     2461 gcgatcgacg agggctgcgc ggacgcgtgg agcgggggct cggccgtcgt cgcctcgggc
     2521 aaggccggcg cgctcgacgg cacgaccgcg ttcttcggct accggtcctt cgggaacgac
     2581 cgatggctca ccctcgtggt cctcgatact gccgcggaca tcgccaccgc gatcgacgat
     2641 ggtacctact atacctcgga cgcgggcgcg ccgaagctcg tgttcgagac tcaacgactt
     2701 tatgatgact ggatcggcgt cagcaccgaa tacgcggagc tgagccagcc gggcggctcg
     2761 cagacatcct tccaggtgca ggtcacggtg accgggcgcc tcggcaactg gctgatgacc
     2821 gaccccaccg acccgccgcg cctgatcggc gaattcgcga ccatcgaccc gaacgccgcg
     2881 acgagcgtgc agggctcgtt cgacgccgcc ttctgcgagg acttcgtcag ccagatcaac
     2941 gactgaccgt caacgacgcc gcaccgcccg cttgaccgcg cccgccaccg cggccagccg
     3001 ccgcagcggc gcgtggccct cgaggcgcac cgcgtcgccc gacgcgatcg tcccgccgcg
     3061 cacgaccgtc gcatacacac cgaccgtcgg catgcgcctg gcgagcaacg cgaacatgcg
     3121 catgttgtgc tccgccgcgg cgcgcagcac ccctggatcg gccgcgagcg gcccctgggc
     3181 gagcgtcgtc atcacgcagc gcgggcacgg aaaggtcacg cacagctgca cctgcgcgcc
     3241 gatcgacagc acctgtccga gccagtcgtt ctcgacgaag ccctgcgccg cgccggtgtc
     3301 gatcacgagg ttcggccgga acctcgcgac cgcgaagtcc gaggccggct gaacctcccg
     3361 tagccgcgcc aggctggccg tgctcacgag gtggaccggc gcgaagtcga agaaggtccc
     3421 cgcgccggcc ccgaccgcca gcgcctcgcc gcgcgcggtg tccagcccgg gctccgcctg
     3481 atcgtacttc gcctgcgggc ccggcgtcgc cgacagccgc accgcccggc cgagggccgc
     3541 ggagagcagg cgctgcgcgt cgccgtcgct gcgcgccgtg gtcccgtcgg gggcagtgat
     3601 ctccaccggc gcgtgccgct gcgcgtccgc cagcggctcg acgaagcgcg cgcgatatcc
     3661 gagcagcgcc ccccacagcc gcgggtgctt ggcgctggcg accctgcccg tctcgtcgtc
     3721 gacgaccgcg aacgcccggt cgccc
//
LOCUS       DASWNJ010001718         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_101828, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001718 DASWNJ010000000
VERSION     DASWNJ010001718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101828"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /gene="atpA"
                     /locus_tag="VGB85_28525"
     CDS             complement(<1..1191)
                     /gene="atpA"
                     /locus_tag="VGB85_28525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEY0138078.1"
                     /translation="MDIRADEISRIIKEQIQGYETKISVEETGTVLTVGDGIARVYGL
                     DNAMAGELLEFPHGVKGMILNLEEGNVGVALLGNDNLVKEGDVVRRTKSIMSVPVGDA
                     LIGRVVNALGVPVDGGPALAGTESRVVELKAPGIIARKSVHEPMQTGLKAIDSLVPVG
                     RGQRELIIGDRQTGKTAVALDCIINQKGQNMICVYVAIGQKVSTVATVVDRLRREGAM
                     EYTIVVAANSSESAPLQFLAPYTGVTMGEFFRDSGRHALLVYDDLSKQAVAYRQLSLL
                     LRRPPGREAYPGDVFYLHSRLLERACKLSDKEGAGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIFLEADLFFSGVRPAINVGISVSRVGGNAQIKAMKQVAGTLRLDLAQYREM
                     QAFAQ"
     gene            complement(1291..1854)
                     /gene="atpH"
                     /locus_tag="VGB85_28530"
     CDS             complement(1291..1854)
                     /gene="atpH"
                     /locus_tag="VGB85_28530"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEY0138079.1"
                     /translation="MIPATLARRYARALLQLADTPMQREVFGRDLATFVRNASSPSAG
                     DASLLTVLDFGIHPLAERRAVLDSVMRRMNLDPTVTKFITLVFERGRIGGLPQISTSF
                     TEMADALAGRQKAVVTSARPLAADSLAKLKASLEKVTGKTILIDTKIDPSLLGGVVAQ
                     VGTYVFDGSIKSQLERMRTSLRNTPQQ"
     gene            complement(1896..>2351)
                     /locus_tag="VGB85_28535"
     CDS             complement(1896..>2351)
                     /locus_tag="VGB85_28535"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HEY0138080.1"
                     /translation="IIRKLVDDNAVRHEDIKRQVESATSSLAEAQAALADFKTRMERV
                     NQDSEQILAQARKAAEADRARLVAEAELEVERFKAGAMAAAAREVAQRRAEIEGEVVD
                     RAIARATELLTTRFNDADHSRLVDDYAVSLADNRTGSDNRTGAAMTARA"
BASE COUNT          333 a          735 c          889 g          394 t
ORIGIN      
        1 ctgcgcgaac gcctgcatct cgcgatactg cgcaaggtcg aggcggagcg taccggccac
       61 ctgcttcatc gccttgatct gcgcgttacc accgacgcgc gagaccgaga taccgacgtt
      121 gatcgcggga cgaacgcccg agaagaagag gtctgcctcg aggaagatct gaccgtccgt
      181 gatcgagatg acgttggttg ggatgtacgc cgagacgtcg ccggcctggg tctcgatgat
      241 cggcagcgcg gtcagcgagc cggcgccctc cttgtcggag agcttgcagg cgcgctcgag
      301 caggcggctg tgcaggtaga acacgtcgcc cgggtacgcc tcgcggcccg gcgggcggcg
      361 cagcagcagc gagagctggc ggtaggcgac ggcctgcttc gacaggtcgt cgtacacgag
      421 cagcgcgtgg cggccgctgt cgcggaagaa ctcgcccatg gtcacgccgg tgtacggggc
      481 caggaactgc agcggggccg actccgagga gttggcggcg acgacgatcg tgtactccat
      541 cgcgccttcg cggcgcaggc gatcgacgac ggtcgcgacg gtcgagacct tctgcccgat
      601 cgccacgtac acgcagatca tgttctgacc cttttggttg atgatgcagt cgagcgccac
      661 cgcggtcttg ccggtctggc ggtcgccgat gatcaactcg cgctggccgc ggccgacggg
      721 caccagcgag tcgatcgcct tgaggccggt ctgcatcggc tcgtgcaccg acttgcgggc
      781 gatgatgccg ggcgccttga gctcgacgac gcggctctcg gtgccggcga gggcggggcc
      841 gccgtcgacc ggcacgccga gcgcgttgac gacgcggccg atcagcgcgt caccgacggg
      901 cacgctcatg atcgacttgg tgcggcggac gacgtcgccc tccttgacga ggttgtcgtt
      961 gcccagcagg gcgacgccga cgttgccctc ctcgaggttg aggatcatcc ccttgacccc
     1021 gtgcgggaac tcgagcagct cgccggccat cgcgttgtcg aggccgtaga cgcgggcgat
     1081 gccgtcgccc acggtcagga ccgtgccggt ctcctcgacc gagatcttgg tctcgtagcc
     1141 ctggatctgc tccttgatga tccggctgat ttcgtcggct ctgatgtcca tgggggtctc
     1201 tcgtgtttcc ggggtggcgc ggcggtaagg aggtccaggc ccgaagggcc gtggagcgat
     1261 caggcccgaa gggccgtgga gcgagtcagc ctattgctgt ggggtgttgc gcaggctggt
     1321 acgcatgcgc tcgagctggg acttgatgct gccgtcgaac acgtaggtgc cgacctgggc
     1381 gacgacgccg ccgagcagcg aggggtcgat cttggtgtcg atgaggatcg tcttgccggt
     1441 gaccttctcg agcgaagcct tgagcttggc cagcgagtcg gcggcgagcg gacgggccga
     1501 ggtgacgacc gccttctggc ggccggcgag ggcgtcggcc atctcggtga aggaggtcga
     1561 gatctgcggc aggccgccga tgcggccgcg ttcgaacacc agcgtgatga acttggtcac
     1621 cgtcgggtcg aggttcatgc gacgcatgac cgagtcgagc accgcgcggc gctcggccag
     1681 cgggtggatc ccgaagtcga gcacggtcag cagagaagcg tcaccggcag aggggctgct
     1741 ggcgttgcgc acgaaggtcg cgaggtcgcg gccgaagacc tcgcgctgca tcggcgtgtc
     1801 ggcgagctgc aggagcgcgc gagcgtagcg gcgagcgagc gtggcgggga tcatccgggt
     1861 cctttgcgat cagagggtca gggtaaatcg cgggctcagg cgcgcgccgt catggcggcg
     1921 cccgtgcggt tgtcgctgcc cgtgcggttg tcggcgagcg agacggcgta gtcgtcgacg
     1981 aggcggctgt ggtcggcgtc gttgaagcgc gtggtcagca gctcggtcgc gcgggcgatc
     2041 gcccggtcga cgacctcgcc ctcgatctcg gcgcggcgct gggcgacctc gcgggcagcg
     2101 gcggccatcg ccccggcctt gaagcgctcg acctcgagct cggcctcggc gacgaggcgg
     2161 gcgcggtcgg cctcggcggc cttgcgggcc tgcgcgagga tctgctcgct gtcctggttg
     2221 acgcgctcca tgcgggtctt gaagtccgcg agcgcggcct gggcctcggc gaggctcgag
     2281 gtcgcggact cgacctgacg cttgatgtcc tcgtggcgga cggcgttgtc gtcgaccagc
     2341 ttgcggatga t
//
LOCUS       DASWNJ010001719         3134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_626568, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001719 DASWNJ010000000
VERSION     DASWNJ010001719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626568"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="VGB85_28540"
     CDS             complement(<1..1232)
                     /locus_tag="VGB85_28540"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0138081.1"
                     /translation="MPGHDDPGARGPAVRRQPGPSSPLSLRSGGHQADAQRPQHRGGH
                     LRGDSLRTILRHHAIDPLPWREGAVLVADLLRALVELHGAGVLHRDLKPGNAIVTRGP
                     DRMTLVLIDLGLAADLKDPSLHPNRRFGSPPYLAPERWRGEAADARSDVYSVGAIFYE
                     MLTGTTPFLASERVELREQHLHSPVVPPRERAPRAQIPEEIQSLVLRALAKRRDDRFA
                     DATAFLGELEETTERLIYRMPRGYQLVPRDLRGWDASANDSTTEAPAAVRAPRAAKTD
                     AGRSQRRAALLVGSVVGAVLATGVLYLRPLASSVTSELHPGAGSSAPEVQPVPGLPPP
                     GASTSSLAGAQPEACDASERSDEPRRPDEPQRVTVARERDTKRRNSMPGAGPALDLSL
                     SEQARRVATEQRVLVGLRA"
     gene            1288..2973
                     /locus_tag="VGB85_28545"
     CDS             1288..2973
                     /locus_tag="VGB85_28545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138082.1"
                     /translation="MSRLTIALLVAMAGCADNFDEDLKAVQEAPFLSTGGTTGGPPTT
                     GGEHGMDPDTSTSSAGAGGDDATTDTGADATTTAGEGEGESSGGSGGILPPPAILEVD
                     MPGKVALAGPVEFTVTTEHASSARARLDGVDIGALHDKGGGIFSGTVAVFGSVDNGPH
                     VLEVIAEREALSDHRSVQFVVGTPAPGSVGWVMAGPVGSHTRRIALTPERDVIEAGLK
                     VIAGVERPVIRKRSGVDGSELWGEGTIVLDEREGRAVDVAVTPDGRMWVAMNVREAAN
                     KWRPRIVLLDANGKFLNVEVPAEAGQTVTGVDNDGSGGCVAVGFVGSGLGDTDVAIWR
                     MNGEHVPVLSGKAWDYQPAGELEAHKFTDLATDVVVNEGVAWIVGLSVGKHGDFLATR
                     SRGFILRVDIDTAGVLGPVIIAPDSALWTQSKFLGAAMHPDGILVTGNGCNGACDTQR
                     VETALYTAAGARTWFLPEDPEAAVAYGSAVALNSHGGVAVAATIREGAALRGFLLGRV
                     VHGDLAMFSVPFPASKEDSEATGVAIDAFDRVFGAGYRTFGGVTEARAVLIQP"
BASE COUNT          450 a         1018 c         1204 g          462 t
ORIGIN      
        1 gctcgcaggc cgacgagcac gcgctgctcg gtggcgacgc ggcgcgcctg ctcgctcagc
       61 gagaggtcga gggcgggccc cgccccgggc atggagttgc gacgcttcgt gtcgcgctcg
      121 cgggccaccg tcacccgctg cggctcgtcg ggcctgcgcg gctcgtcgga tctctcggac
      181 gcgtcgcatg cctccggctg cgctccggcc agcgagctcg tcgaggcgcc cggcggcggg
      241 agtcctggca ccggctgcac ctcgggcgct gacgatcctg ctcccgggtg aagctcggag
      301 gtcaccgagc tcgccagtgg tcgcaggtag agaacgccgg tggcgagcac ggcgcccacg
      361 accgatccca cgagcaacgc tgctcgccgc tgcgagcgac ccgcgtcggt cttcgccgcg
      421 cgtggggccc ggaccgcggc cggggcttcc gtcgtcgagt cgttcgccga ggcatcccat
      481 cctcgcaggt cccgtggcac gagctggtag ccgcgcggca tgcggtagat cagccgctcc
      541 gtggtctcct cgagctcgcc gagaaaggcc gtcgcatcgg cgaagcggtc gtcgcggcgc
      601 ttggcgagcg cgcgaagcac gagcgactgg atctcctcgg ggatctgggc ccgcggggcc
      661 cgttcgcgcg gcggcaccac tggcgagtgc aagtgttgct cgcgcagctc gacgcgctcc
      721 gacgcgagaa acggcgtcgt gccggtcagc atctcataaa aaatggcgcc gacggagtag
      781 acgtccgagc gcgcgtccgc agcctcgcct cgccagcgct cgggcgcgag gtaaggcggc
      841 gagccgaagc ggcggttcgg gtgcaggctc gggtccttga ggtcggcggc gaggccgagg
      901 tcgatcagca cgagggtcat ccggtccggg ccgcgggtga cgatcgcgtt gccaggcttg
      961 aggtcacggt gcaggacgcc ggctccgtgc agctcgacga gcgcgcgcag cagatcggcg
     1021 acgagcacgg cgccctcgcg ccagggcagc gggtcgatcg cgtgatgccg caggatcgtc
     1081 cgcaacgagt cgccgcgcag gtgtccgccg cggtgctggg ggcgctgggc gtcggcctgg
     1141 tggccgccgg agcgcagcga cagcgggctc gacgggcccg gctggcgccg tacggcgggc
     1201 ccgcgggcgc cgggatcgtc gtggccgggc aattctgagg gggcgccccg ggcgacgagc
     1261 ggatgcgcgc gtgatacctt ggggtcgatg tcgcgcctca ccatcgccct gctcgtcgcc
     1321 atggccggtt gtgcggacaa cttcgacgag gacctcaagg ccgtgcagga ggccccgttt
     1381 ctgtcgacag gcgggacgac gggcgggcca cccacgacgg gcggggagca cggaatggac
     1441 ccggacacga gcacctctag tgccggcgcc gggggcgacg atgcgacgac cgacaccggc
     1501 gcggacgcca ccacgacggc aggcgagggg gagggcgaga gcagcggcgg aagcggcggc
     1561 attttgccgc caccggcgat cctggaggtc gacatgcccg gcaaggtggc gctcgccggg
     1621 ccggtggaat tcaccgtgac gaccgagcac gcgagctcgg cgcgggcccg actggacggc
     1681 gtcgacatcg gcgccctgca cgataaaggt ggggggatct tctccggtac ggtggcggtc
     1741 ttcggctcgg tggacaacgg accacacgtg ctcgaggtga tcgcggagcg cgaggcgctg
     1801 tccgatcatc ggtctgttca gttcgtggtc ggcacgccgg cacccggctc ggtcggctgg
     1861 gtcatggccg ggccggtcgg ctcgcatacg cggcggatcg cgctgacgcc cgagcgcgac
     1921 gtgatcgagg ccggcttgaa ggtgatcgcc ggcgtcgagc ggccggtgat ccgcaagcgg
     1981 tccggggtcg acggctccga gctgtggggc gagggcacga tcgtgctcga cgagcgcgag
     2041 ggccgggccg tcgacgtcgc ggtgacgccc gatggacgga tgtgggtggc gatgaacgtg
     2101 cgtgaagccg cgaacaagtg gcgcccgcgg atcgtgctgc tcgacgcgaa cgggaaattt
     2161 ttaaatgtcg aggtgcccgc cgaggccgga cagaccgtca ccggcgtcga caatgatggt
     2221 agcggcggct gcgtcgcggt cggcttcgtc ggctcggggc tgggggacac ggatgtcgcc
     2281 atctggcgca tgaacgggga gcacgtgccg gtgctgagcg gcaaggcgtg ggactaccag
     2341 ccggctggag agttggaggc gcacaagttc acggacctgg cgaccgatgt cgtggtcaat
     2401 gagggggtcg cgtggatcgt cggcctgagc gtcggcaaac atggtgactt cttggccaca
     2461 cgcagccgcg ggttcatcct gcgcgtcgac atcgacacgg ccggagtgct cggacccgtc
     2521 atcatcgcgc cggactcggc gttgtggaca cagagtaaat tcctcggggc tgccatgcat
     2581 ccggacggca tcctcgtgac cggcaacggc tgcaatggcg cctgcgatac acagcgcgtc
     2641 gagacggcgc tctacacggc cgcgggggca cgtacatggt ttttgcccga ggatccggag
     2701 gccgcggtgg cgtacggcag cgccgtcgct ctcaactcgc acggcggcgt cgccgtcgcc
     2761 gccacgatac gcgagggagc cgcgcttcgg ggcttcctgc tcgggcgggt tgtccacggc
     2821 gaccttgcca tgttcagtgt gccgttcccg gcgagcaagg aggactcgga ggcgaccggg
     2881 gtcgccatcg acgccttcga tcgggtgttc ggtgccggct atcgcacctt tggcggggtc
     2941 accgaggcac gggctgtcct cattcaacca tagcgcctcg gcgagggacc tgtatcgagg
     3001 tcgtcactgc gggacgaagt cgctgcacgc ctccgcgaca cggctcgccg ctttctcgaa
     3061 gaacgggatg tagctggtcg cgcacgcatc tccctcttca tggaagggga acatgttgat
     3121 caggtcgcgt agcc
//
LOCUS       DASWNJ010001720         2254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_66220, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001720 DASWNJ010000000
VERSION     DASWNJ010001720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66220"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1593)
                     /locus_tag="VGB85_28550"
     CDS             complement(<1..1593)
                     /locus_tag="VGB85_28550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138083.1"
                     /translation="MVAALLGVLLGVVGPANAAAQALTVAQERTLPIVGDGVEVRQRT
                     YALHGVPVRRAYATIVAQAGGERVVDEVVPDEAPELLPAQARIGAAQAVELARDHLRR
                     GALQPKAEGPGTLVYIVILGVPVLAYEVDMALDLDGPEPSKKTLWISADSGIVLDEWE
                     HVRASRARVFLTNPARTPEPIVVELSGVHATEAGQPLVGDRVQSFNCTLDPQDPETLP
                     AWWKEGKCYPVHTALSNADGDFFVDMPDIIFPEDNADNEDAYAELSMYWHAERFLDRM
                     AALGVPEFKCELSTMLANYVDPKLSPSYPDLDYTPLNNAYWTNTCDPAKGVTMIFGQG
                     SDVDFGYDGDVVYHELGHGMVSLLTPDGLGARTNRSDGQLADAGGLNEAIADYFSVML
                     TSDPYLGDYVARFWPGYGEAIRNAENSKTCPDDTIGQVHNDGEPFMAALWATRKRVGG
                     EKLDPLVIKMLTRLPIDADLETASWTVLELAEDAGWSATEIDTLIRAFDTRGLYDCPR
                     VIVDPTRVSAGRTMYLRPKGAAV"
     gene            1752..>2254
                     /locus_tag="VGB85_28555"
     CDS             1752..>2254
                     /locus_tag="VGB85_28555"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEY0138084.1"
                     /translation="MLPSPDPAPVRPRPRRRPVLALALALAALVPRPAAASSAWLHAL
                     AIAGPAIATPPATPPAEPLTLQARIDAVLAATFLAGATVGVHVVDLADGRVLYARNAE
                     LAQNPASNVKLVTTAAALALLGPEHRYVTRVYVDPKALKGHTIDGDLYLRGGGDPSLV
                     TGDLYQLA"
BASE COUNT          296 a          880 c          740 g          338 t
ORIGIN      
        1 caccgcggcg cccttgggtc gcaggtacat cgtgcggccg gcgctgacgc gcgtcggatc
       61 gacgatcacg cgcgggcagt cgtagagccc gcgcgtatcg aaggcgcgga tcaacgtgtc
      121 gatctcggtc gccgaccagc cggcgtcctc ggcgagctcg agcaccgtcc agctcgccgt
      181 ctcgaggtcc gcgtcgatcg gcaggcgcgt cagcatcttg atcaccagcg ggtcgagctt
      241 ctcgccgccg acccgcttgc gggtcgccca cagcgccgcc atgaacggct caccgtcgtt
      301 gtgcacctgg ccgatcgtgt cgtccgggca agtcttcgag ttctcggcgt tgcggatcgc
      361 ctccccgtag ccgggccaga accgggccac gtagtccccg aggtacgggt ccgaggtcag
      421 catcaccgag aagtagtcgg cgatcgcctc gttgagcccg cccgcgtcgg cgagctgtcc
      481 gtcgctgcga ttggtccgcg cgccgaggcc gtccggcgtc agcagcgaca ccatcccgtg
      541 cccgagctcg tggtagacga catcgccgtc atacccaaaa tcgacgtcgc tgccctggcc
      601 gaagatcatc gtcacgccct tggccgggtc gcaggtgttg gtccagtagg cgttgttgag
      661 cggcgtgtag tcgaggtccg ggtagctcgg cgacagcttg gggtcgacgt agttggcgag
      721 catcgtcgac agctcgcact tgaactccgg caccccgagc gccgccatgc ggtcgaggaa
      781 tcgctccgcg tgccagtaca tcgacagctc ggcgtacgcg tcctcgttgt cggcgttgtc
      841 ctccgggaag atgatgtcgg gcatgtcgac gaagaagtcg ccgtccgcgt tcgacagcgc
      901 cgtgtggacc gggtagcact tgccctcctt ccaccacgcc ggcagcgtct ccgggtcctg
      961 cgggtcgagc gtgcagttga agctctgcac gcggtcgccg acgagcggct gcccggcctc
     1021 cgtcgcgtgc acgcccgaca gctcgaccac gatcggctcg ggcgtgcgcg ccgggttggt
     1081 caggaacacc cgggcccgcg aggcccgcac gtgctcccac tcgtcgagca cgatcccgct
     1141 gtcggccgag atccacagcg tcttcttcga cggctccggg ccgtcgaggt cgagcgccat
     1201 gtcgacctcg taggcgagca ccgggacccc gaggatcacg atgtacacca gcgtgcccgg
     1261 gccctccgct ttcggctgca gcgcgccgcg ccgcaggtga tcccgcgcga gctcgacggc
     1321 ctgcgccgcg ccgatccggg cctgcgccgg cagcagctcc ggcgcctcgt ccggcaccac
     1381 ctcgtcgacg acgcgctcgc cgccggcctg cgcgacgatc gtcgcgtacg cgcgccggac
     1441 cggcaccccg tgcagggcat acgtgcgctg gcggacctcc accccgtcgc cgacgatcgg
     1501 cagcgtgcgc tcctgcgcga ccgtgagcgc ctgcgcagcc gcgtttgccg gccccacgac
     1561 accgaggagc acgccgagga gcgcggcgac cacccccgtg gcgatcccga tcccggttcc
     1621 ggtccggagc gccgtgcgcc gcgcgcgagc caatgtgcgt accctggaca tccgcgcccc
     1681 cgatcgcggg caggatagcc caaagatcgg aagtttggcc acgggcccgt ccccggctac
     1741 cgtgccgggt ggtgttgccc tcgcccgatc cggcccccgt ccgcccgcgc ccgcgacggc
     1801 gccctgtcct cgccctggcc ctcgccctgg ccgccctcgt gccgcgcccg gcggccgcgt
     1861 cgagcgcctg gttgcacgcc ctggcgatcg ccggcccggc gatagccacc ccacccgcca
     1921 cgccgcccgc cgagccgctg accttacagg ctcgcatcga cgccgtgctc gccgcgacct
     1981 tcctcgccgg cgcgaccgtc ggcgtgcacg tcgtcgacct cgccgacggc cgcgtcctct
     2041 acgcccgcaa cgccgagctg gcgcagaacc ccgccagcaa cgtcaagctc gtgaccaccg
     2101 ccgccgcgct cgcgctgctg ggccccgagc accgctacgt cacccgcgtc tacgtcgacc
     2161 cgaaggctct caagggccac acgatcgacg gcgatctcta tcttcgcggc ggcggtgatc
     2221 cctcgctcgt caccggcgac ctctaccagc tcgc
//
LOCUS       DASWNJ010001721         3830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_209085, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001721 DASWNJ010000000
VERSION     DASWNJ010001721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209085"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1541
                     /locus_tag="VGB85_28560"
     CDS             <1..1541
                     /locus_tag="VGB85_28560"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HEY0138085.1"
                     /translation="VEVLEVALQAARGLAAAHDAGLVHRDVKPDNIMIGDDGRVRVMD
                     FGLARAARSRADDDELISQPSPAPLTAVGAVVGTPGYMAPEQLRGAVVDVSSDIYSFC
                     VVVYELVTGVRPPAEPADLQAALARRRAPRWLAALVLRGLQADPAARWPTMHALIAVL
                     AAALRPEVRRTRVRVLTAATLLAVSWLAAWRAHVVIDQHQRARFGEEVEKIESAVQQR
                     MTAYVQVLRGGLGLFVVAGEGDLDRWLRYVTTLQLGERYPGFKSLSFAAAVTPAELPG
                     FVARVRGWPLPPGITDPRLIREFSIRSPVKTSSAPPIHSPIVFVAPMVRENERVLGVD
                     MMQEPMRRAAMERAAEVDAAVLSPRVNLAGQGDLKAGFIAYLAVRRDGALVGWLTAAF
                     LAEDFMRGLLGDGPPALELEVYDGTTAVPEALLYSTAGIADTGLPLALPADPTSVLEQ
                     VGGVELPGRVWTLRYRATSEFSPLTDRLVPWLIVISGLFAATLFYAMSRASAQLRPGP
                     RTRA"
     gene            1588..2496
                     /locus_tag="VGB85_28565"
     CDS             1588..2496
                     /locus_tag="VGB85_28565"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0138086.1"
                     /translation="MGRLRQTDGADRLLRGLNFIGRSRACTICLDRPEVSGEHAVLRW
                     TGSGWEIKDLHSRNGVYVDGHRLPAGERAAIGPGCRLGFGRDGGHELVDAGEPIAFAD
                     PLTGAAPLTAVGGILALPDPDAPEVTIFRAGADWAIDRSGETAVVGDGELVRVGDQVW
                     RVHLPELLTPTDVAADLAPQLDDLLLRFSVSRDEEYVELVAVHDRQTFDLKVRSHHYP
                     LLLLARARLRQHALPPARQGWVHQHELLELLRVDRNQLHLDIFRVRRQLGALGIRDAA
                     RVVERRGGTSQLRIGASRIEIVPLGG"
     gene            2645..2854
                     /locus_tag="VGB85_28570"
     CDS             2645..2854
                     /locus_tag="VGB85_28570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138087.1"
                     /translation="MDAASLYLVGKKLAVLAEPAMASPEGLPAEPTMERSPSSSTPPG
                     GSGPACSRPSSWSTSSLFAREHPAS"
     gene            2868..3539
                     /locus_tag="VGB85_28575"
     CDS             2868..3539
                     /locus_tag="VGB85_28575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138088.1"
                     /translation="MRIYHVIVAAVVLGTALPIGLHYAVHGVVSPHQVGIAFFLWLNT
                     IVALWEICLFLRIDLIEAQYAGFRTEYAGRELDRPKDFFAARIPVSKVLSPSTWAELW
                     SSYALFDPSYADRRSFGFYIDIGNGFSTLVPSLLFTYGMTFHVLSPRVLGVIGLLICY
                     QMWYGTVVYFTSYVLNKRYVGHSPISVALFVGLSNGMWFTFPIWAMWAALRMIDEGSY
                     AVFMD"
     gene            complement(3546..>3830)
                     /locus_tag="VGB85_28580"
     CDS             complement(3546..>3830)
                     /locus_tag="VGB85_28580"
                     /inference="COORDINATES: protein motif:HMM:NF022135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEY0138089.1"
                     /translation="PERDLATLRAGERFVVTVTFEGAPLAGAMVEAFVRPAGASAPRG
                     QKAVSDGQGRVEFTATEAGAWLVRTVHMRRCERCTDADWESFWAGYSFAL"
BASE COUNT          493 a         1413 c         1330 g          594 t
ORIGIN      
        1 tcgtcgaggt gctggaggtc gcgctgcagg ccgcccgcgg gctggcggcg gcccacgacg
       61 ccgggctggt ccaccgcgac gtcaagcccg acaacatcat gatcggcgac gacggccgcg
      121 tgcgcgtgat ggacttcggg ctggcccgcg ccgcccgctc gcgcgccgac gacgacgagc
      181 tgatatcgca gccctcgccc gcgccgctga cggccgtcgg cgcggtggtc ggcacgccgg
      241 gctacatggc accggagcag ctgcgcggcg cggtggtcga cgtcagcagc gacatctaca
      301 gcttctgcgt cgtcgtgtac gagctggtga ccggcgtccg cccgcccgcc gaaccggcgg
      361 acctgcaggc cgcgctggcc cgacgccgcg ccccgcggtg gctggcggcg ctggtgctgc
      421 gtgggctgca ggccgacccg gccgcgcgct ggccgacgat gcacgcgctg atcgccgtgc
      481 tcgcggcggc gctgcgcccc gaggtccggc gcacgcgggt ccgcgtgctg acggccgcga
      541 cgctgctcgc cgtcagctgg ctcgcggcct ggcgggcgca cgtcgtcatc gaccagcacc
      601 agcgcgcgcg cttcggcgag gaggtcgaga agatcgagtc cgcggtccag cagcgcatga
      661 cggcctacgt ccaggtgctg cgcggcggcc tcggcctgtt cgtcgtcgcc ggcgagggcg
      721 acctggaccg ctggctgcgc tacgtgacga ccctgcagct cggcgagcgc taccccggct
      781 tcaagtcgct gagcttcgcg gccgccgtca cgccggccga gctgccgggg ttcgtcgcgc
      841 gcgtgcgcgg atggccgctg cccccgggca tcactgaccc gcggttgatc cgcgagttct
      901 cgatccgctc gccggtgaag acgtccagcg cgccgccgat ccactcgccg atcgtgttcg
      961 tggcgccgat ggtccgcgag aacgagcgcg tcctcggcgt cgatatgatg caggagccga
     1021 tgcgccgggc cgcgatggag cgcgccgccg aggtcgacgc cgccgtcctg agcccgcgcg
     1081 tcaacctggc cggacagggc gacctcaagg ccggctttat cgcctacctc gcggtccgtc
     1141 gcgacggcgc gctcgtcggc tggttgaccg ccgcgttcct cgccgaggac ttcatgcgcg
     1201 gcctgctcgg cgacggcccg ccggcgctcg agctcgaggt ctacgacggc acgaccgctg
     1261 tccccgaggc gctgctctac tcgacggccg gcatcgcgga caccgggctg ccgctcgcgc
     1321 tgccggccga cccgacgtcg gtgctcgagc aggtcggtgg cgtcgagctc ccggggcggg
     1381 tgtggacgct gcgctatcgg gcgacgtcgg agttctcgcc gctgaccgac cgcctcgttc
     1441 cgtggttgat cgtgatcagc gggctgttcg ccgcgaccct gttctacgcg atgtcgcgcg
     1501 ccagcgcgca gctgaggccc ggaccgcgca cgcgggcctg ataaacggtc tggcggacgc
     1561 gtgtcggcct cggcaaagtg accgccagtg ggacgactgc gacagaccga cggcgcggac
     1621 cggctgctgc gagggctcaa tttcatcggg cgctcgcggg cctgcacgat ctgcctcgac
     1681 cgccccgagg tgtccggcga gcacgccgtg ctgcgctgga ccggcagcgg ctgggagatc
     1741 aaggacctgc acagccgcaa cggcgtgtac gtcgacggcc accgactgcc cgccggcgag
     1801 cgcgctgcga tcggccccgg gtgtcgcctc ggcttcggtc gcgacggcgg gcacgagctc
     1861 gtcgacgccg gcgagccgat cgccttcgcc gacccgctca ccggcgcagc gccgctgacg
     1921 gccgtcgggg ggatcctcgc gctgccggac ccggacgccc ccgaggtgac gatcttccgc
     1981 gccggcgccg actgggcgat cgaccgctcc ggtgagaccg cggtcgtcgg cgacggcgag
     2041 ctcgtccgcg tcggcgatca ggtctggcgc gtgcacctgc ccgagctgct gacgccgacc
     2101 gatgtcgccg cggacctcgc cccccagctc gacgacctgc tgctgcgctt cagcgtcagc
     2161 cgcgacgagg agtacgtcga gctggtcgcc gtgcacgacc gccagacctt cgacctcaag
     2221 gtccgcagcc accactaccc gctgctcctg ctggcgcgcg cccgcctgcg ccagcacgcg
     2281 ttgccgcccg cgcgccaggg ctgggtccat cagcacgagc tgctcgagct gctgcgcgtc
     2341 gatcgcaacc agctgcacct cgatatcttc cgcgtgcgcc gccagctcgg cgcgctcggg
     2401 atccgcgacg cggcccgcgt cgtcgagcgc cgcgggggca cgagtcagct gcggatcggc
     2461 gcgtcgcgga tcgagatcgt cccgctcggc ggctgagatt ccggcgaggc gccgcggagc
     2521 tcctgcgtgc atatcggatg agacatctga aaaagatgcg gagcgcacgc tccggcgcac
     2581 tgcgcgcgga acggagcgcg aggtctcacc ttgacaagaa catcgttctt cgtgagtaag
     2641 gggtatggac gcggcttcgc tgtatctcgt cggcaagaag ctggccgtgc tcgccgaacc
     2701 ggcgatggcg tccccggagg ggctgcccgc cgagcccacg atggagcgga gcccctcctc
     2761 gagcacgcca ccgggcggga gcgggcccgc gtgctcgcga ccgtcgagct ggtcgacgag
     2821 ctcattgttc gcgcgcgagc acccggcgag ctgaggatcc cgccgtcatg cgcatctatc
     2881 acgtcatcgt cgccgccgtc gtcctcggca cagcgctccc gatcggcctg cattacgccg
     2941 tgcacggcgt cgtgagcccg catcaggtcg gcatcgcgtt cttcctgtgg ctaaacacga
     3001 tcgtcgcgct gtgggagatc tgcctgttcc tgcgcatcga cctgatcgag gcgcagtacg
     3061 ccggctttcg caccgagtac gccggccggg agctcgaccg cccgaaggac ttcttcgccg
     3121 cgcgtatacc cgtgtcgaag gtgctctcac cgagcacgtg ggcggagctg tggtcgtcct
     3181 atgcgctgtt cgacccgtcc tacgcggacc ggaggtcctt cggcttctat atcgacatcg
     3241 gcaacggctt ctcgaccctt gttccctcgc tgctgttcac gtatggcatg accttccacg
     3301 tgctgtcgcc gcgcgtgctg ggggtcatcg ggctcctgat ctgctaccag atgtggtacg
     3361 gcacggtcgt ctacttcacc agctacgtgc tgaacaagcg ctacgtcggc cactcgccga
     3421 tcagcgtcgc cctgttcgtc gggctctcga acggcatgtg gttcacgttc ccgatctggg
     3481 cgatgtgggc ggccctgcgt atgatcgacg agggctcgta cgcggtgttc atggactgac
     3541 caccgtcaca gcgcgaagct gtatcccgcc caaaagctct cccaatccgc gtccgtgcag
     3601 cgctcgcagc gtcgcatgtg caccgtacgc acgagccacg cgcctgcctc cgtcgccgtg
     3661 aattcgaccc tgccctgccc gtccgagacc gccttttgcc cgcgcggcgc gctggccccc
     3721 gcgggacgaa cgaacgcctc gaccatcgcg cccgccagcg gcgccccctc gaaggtcacg
     3781 gtcaccacga agcgctcacc cgcccgtagc gtcgcgaggt cgcgctcggg
//
LOCUS       DASWNJ010001722         8907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_30298, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001722 DASWNJ010000000
VERSION     DASWNJ010001722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 8907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8907
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30298"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VGB85_28585"
     CDS             complement(<1..367)
                     /locus_tag="VGB85_28585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY0138090.1"
                     /translation="MQILERRTYRGPNLYAHFPVIRLNLDLGELERHPSATIPGFVDG
                     LLTALPSLQSHTCSYGTEGGFVRRLREGEGTWLGHVLEHVAIEVQVLTGAKVTFGKTR
                     ESGAQGQYFVVYEYEEERVG"
     gene            557..1321
                     /locus_tag="VGB85_28590"
     CDS             557..1321
                     /locus_tag="VGB85_28590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138091.1"
                     /translation="MSLEFVDVATARAARGVRMVVSGMVPSPWSEAAKGLFHVQRVPV
                     LAVRAAREDPEIAAFSKADNVPSVMYDDEPPRTSWAAIVMLAERLGAPGLLVPTDGER
                     RVRMIGLIHEIAGEDGLGWSARLRMIDAALTSEGARGFPLPVARYLAGKYGHSSERAA
                     RAHGRMREVLRALVQALGDDDYHGGHVPNALDVYSATFLTPMTTISEEDCPKMVPPLR
                     QAFAVVRDELGDEVPEVLLAHRRRMFERHLAWPIAL"
     gene            complement(1323..2165)
                     /locus_tag="VGB85_28595"
     CDS             complement(1323..2165)
                     /locus_tag="VGB85_28595"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0138092.1"
                     /translation="MRVAWATDIHLDFLADPQVLDFAATLTASSPDAVVLSGDLSHAE
                     QLEHHLRLLASAISCPIYFVLGNHDYYGSSIAAVRQAVAELGARKPRLRWLPAHGVVT
                     LSEKTAMVGVDGWADSRLGDAEHTPVILNDFIHIEDLVATLDPLVRATPALLRSTDRS
                     QLHTKLRALGDDEAARCRELLAPALASHEHVLVVTHVPPFAAACWHDGELSDDKWLPY
                     FSCAAVGEVLRDLAITHPHRTLTVLCGHTHGAGEAQIEANLRVLTGGAVYRHPHLQQL
                     LELA"
     gene            complement(2180..3151)
                     /locus_tag="VGB85_28600"
     CDS             complement(2180..3151)
                     /locus_tag="VGB85_28600"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY0138093.1"
                     /translation="MRLALACACLALACDRPPAPVAAAQPAPPAPPPAPPPVQPPAPP
                     APVPKASPSPDGALPCLDPPPPGLACIPGGPFIRGSDPREDGPENTRPRATVWLQTYY
                     VDIYEVTHAQYKACESSKNCPPAGPRYDDYDRPRQPIVGVSWYDAIAYCTAQGKHLPT
                     EAQWEKAARGPDGALHPWGDEPATCERAIIKEKGRRSCGVRQQGERPDKGRTFEVGAR
                     PPGAHGLHDMSGNAWEWVADWYSPSCSRCGSACAGTDPRGPCDGAEPCARHHEKIVRG
                     GSWYWEASYATATYRRAHFPNNRPYHHYGFRCAASPAEAAALASPAG"
     gene            3337..4293
                     /locus_tag="VGB85_28605"
     CDS             3337..4293
                     /locus_tag="VGB85_28605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138094.1"
                     /translation="MTNIMIVSQLLVLWSGQEVPGPAADPSEVLAPIEQAPVPVVEPK
                     PSMRVVPVAAPAVVLQNDASAAGDWSRLRGRRRFGRRLAGGGFAGFGAAYVGNFIAGV
                     RSGEVGEDPTTMAGLFPVIGPFLLAGWTGPNTPGLKTFFVLDGVAQVGFLAMGITGVV
                     IAKRSRRALAQPRFFGLNGQFEHRLDNDPDLRRRGRLGLGLALGGFIGFGVTYLSTVM
                     TMLGPKDEGLLANEPNVQRPWVIAPLIGPFAIAGQAEAAGTKALMAVLGLVQVGSLAT
                     GITGAVIRVRRVRDAESRVLKNVSIAPWHSGTTTGLSLRGQF"
     gene            complement(4467..6179)
                     /locus_tag="VGB85_28610"
     CDS             complement(4467..6179)
                     /locus_tag="VGB85_28610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138095.1"
                     /translation="MLRLRSVLVIQMLLAAGAAAALVPLPGCSTGCRDPWLTPEAFDL
                     PVEATILGDVWSTYDGSQVLVGSGGVIVEDVTSTPRIHRPVTADLRGVTITRELVVAV
                     GDGGTVVVASRGSDEWQVIDLGTTADLHAVTTFETEAGEEIVVVVGEGVMFARAAETG
                     AWSAVPAPKGGWGSLRGVDAQPYTGTIVAVGRGGAIWRASDPNGTWQREDSGTSEDLT
                     IVRLGLIGGTNGTLLRLGDDGTLHKFDLGTDSDVLDVHPVVLTADGRVFEFEAYEDAP
                     SGAIYTLGPGLRVVIEDGESEYGLVALGEPGKHRHLGNTCVPYEGRPFIVEDVARTAA
                     VVRRGDWGAEDRTAEVLPAATRAALAEGWTQAALAEHASIASFARAVLELMSLGAPAE
                     LLRATQAALADEVEHAARSFAQASRYRGAPCGPGPLVVDAASLARAGDPAAIAVAVFE
                     EGCVGESVSAAQAALAAEECRDTEVAALLRDIAREEGQHAALAWQTLRWLVDTYGELV
                     AAPLRRAAAGLTAPLAMVGGPDLRAHGRLSAAARAAIHRAVVRDVVRPIADALLGRRE
                     AVQA"
     gene            complement(6287..7240)
                     /locus_tag="VGB85_28615"
     CDS             complement(6287..7240)
                     /locus_tag="VGB85_28615"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEY0138096.1"
                     /translation="MQAPPPPGHPGHPGQTSDADASLLAGVCDHVGCDSHALRWQFIG
                     SALGPCRVWQVSGSEPGPGVVVKQFRNDRAFHQERHAYARWLPHLSEETAPLLAAFAA
                     PVRALVLGVVPGRPLAGAVATPDSERAAHERAGRFLRALHDLREPDRDPLPLAAAISQ
                     RYAAWLARVRPTVDATTHARLAAFTALADDPRLFAGARRVPCHRDFTPGNWLVSGSDS
                     DGPILTLDSFFVIDFEHAHLDTPLLDMVKLWTEVWGTRPDLEAAFFAGYGRTLNPQEQ
                     RQLSVLAAMHAMATVSWADEHADLQFAAAGRQALRRVFDSL"
     gene            complement(7243..8016)
                     /locus_tag="VGB85_28620"
     CDS             complement(7243..8016)
                     /locus_tag="VGB85_28620"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEY0138097.1"
                     /translation="MSAAARAHQREIGRVTVTRQARRALLWSVGLTLLLPRIPLLSVL
                     GIPLVWLSTVAHELGHGLTAVALGGSFDRLIIRPDGSGTAFTAGTGTDLAHALVCAGG
                     LVGPAIVAAVWFVLARRPALARLALAVFGAALIAAAVLVGDPGFTQGYLGVVGGLSLV
                     AAFCTSAAWAQILLVFLAVQMALSVFTRSDYLFTEVAHSSAGELASDVAQMSLRLGGP
                     YWAWGLACGLVSLLVLAGGLLWYLRGDSRVSLGALRARR"
     gene            complement(8064..>8907)
                     /locus_tag="VGB85_28625"
     CDS             complement(8064..>8907)
                     /locus_tag="VGB85_28625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138098.1"
                     /translation="AIEVGSLASRVVALKACLVMGIVDGQISPEEWKVLSEFRAALAI
                     DEAQLHSFHELVHRHRQLTMHEYQRRLAAPQLGTLYASEGWQGVMQFFPADVLAAAAS
                     GPTASPSTPSTLSIPSSHGAARENAEVSQRYRQLGSLDEGTLGRELWRYYKEREFELA
                     SDPGTVPDALVEHDIMHIISGYDSSLEGELQTLAFVAGMRQEDPFSALFLVLLQYTAG
                     RKVAPGGQLAGARVLFDHDKILRALDRGRGTAVDFSAKHDFWDIMDQPIAALRERFGV
                     PAVA"
BASE COUNT         1228 a         2931 c         3509 g         1239 t
ORIGIN      
        1 cgccgacgcg ctcctcctcg tactcgtaga cgacgaagta ctggccctgc gccccggatt
       61 cgcgggtctt gccgaaggtc accttggcgc cggtcagcac ctggacctcg atcgcgacgt
      121 gctcgagcac gtggccgagc caggtgccct cgccctcgcg caggcggcgg acaaacccgc
      181 cctcggtccc atacgagcag gtgtgtgact gcaggctggg gagcgcggtg agcaggccgt
      241 cgacgaagcc ggggatcgtc gcggaggggt gccgctccag ctcgcccagg tccaggttga
      301 ggcggatgac cggaaagtgg gcgtagaggt tgggaccgcg ataggtgcga cgctcgagga
      361 tctgcatggg gtgtccgtca gtcggggccg tcggcggtgc tcggctcggg gaggatatgg
      421 tgcggaattc gttcgaggca aattcacggg taggggcggg cctgaggcgc tcctgggccg
      481 atccgggcgt cagtacgcgg gggcggcgca gcagggcgcg attgtgccgg atcgccgggg
      541 cgagcatcat cgccgcatgt ccctcgaatt cgtcgatgtc gcgacggcac gcgcggcgcg
      601 tggcgtacgc atggtggtgt cgggcatggt gccgagcccg tggtcggagg cggccaaggg
      661 cctgtttcat gtccagcggg tgccggtgct cgcggtgcga gcggcccgcg aggacccgga
      721 gatcgcggcg ttctcaaagg cggacaacgt gccctcggtg atgtacgacg acgagccgcc
      781 gcgcaccagc tgggcggcga tcgtgatgct cgcggagcgg ctgggggcgc cgggcctgct
      841 ggtgcccacg gacggtgagc ggcgcgtccg catgatcggg ctgatccacg agatcgccgg
      901 ggaggacggg ctcggctgga gcgcgcgcct gcggatgatc gacgcggcgc tgacgagcga
      961 gggggcgcgg ggcttcccgc tgccggtcgc gcgctacctc gcgggcaagt acgggcactc
     1021 gagcgagcgc gccgcgcggg cgcacggccg catgcgcgag gtgctgcgcg cgctggtaca
     1081 ggcgctcggc gacgacgact atcacggcgg ccatgtgccc aacgcgctcg acgtgtacag
     1141 cgcgacgttc ctgacgccga tgacgacgat ctccgaggag gactgcccga agatggtccc
     1201 gccgctgcgc caggcgttcg cggtggtccg cgacgagctc ggcgacgagg tgccggaggt
     1261 gctgctggcc caccgccggc ggatgttcga gcgccacctc gcgtggccga tcgcgctgtg
     1321 agtcacgcaa gctcgagcag ctgttgcagg tgcgggtggc ggtagaccgc gccgccggtg
     1381 agcacgcgca ggttggcctc gatctgcgcc tcgccggccc cgtgggtgtg gccgcacagg
     1441 acggtcagcg tgcggtgcgg gtgggtgatc gcaaggtcgc gcaggacctc gccgacggcg
     1501 gcgcagctga agtagggcag ccacttgtcg tccgagagct cgccgtcgtg ccagcaggcg
     1561 gcggcgaagg gcgggacgtg ggtgacgacg agcacgtgct cgtgcgaggc gagggcgggc
     1621 gccagcaatt cgcggcagcg ggcggcctcg tcgtcgccga gggcgcgcag cttggtgtgt
     1681 agctgcgagc ggtcggtgct gcgcagcagc gcgggcgtgg cgcggacgag ggggtcgagg
     1741 gtggcgacga ggtcctcgat gtgaatgaag tcgttgagga tgacgggggt gtgttcggcg
     1801 tcgccgaggc gggagtcggc ccagccgtcg acgccgacca tggctgtctt ttcggacagg
     1861 gtgacgacgc cgtgggcggg cagccagcgc aggcgcggct tgcgggcgcc gagctcggcg
     1921 acggcctggc ggacggcggc gatcgagctg ccgtaataat cgtgattgcc gagcacgaag
     1981 tagatcgggc aggagatcgc gctggcgagc aggcgcaggt ggtgctcgag ctgctcggcg
     2041 tgcgagaggt cgccgctgag cacgacggcg tcggggctac tggcggtgag cgtggccgcg
     2101 aagtcgagca cctgggggtc ggcgaggaag tcgaggtgga tgtcggtggc ccaggcgacg
     2161 cgcacgccgc aggataacgt cagcccgccg ggctggcgag cgcggcggcc tcggccgggc
     2221 tcgccgcgca gcgaaagccg tagtggtgat acgggcgatt gtttggaaag tgcgcgcggc
     2281 ggtaggtcgc ggtcgcgtac gaggcctccc agtaccacga gccgccgcgg acgatcttct
     2341 cgtggtgacg cgcgcagggc tcggcgccgt cgcacgggcc gcgcggatcg gtgcctgcgc
     2401 aggcggatcc gcagcgggaa cagctcgggc tgtaccagtc ggcgacccac tcccacgcgt
     2461 ttcccgacat gtcgtgcagg ccgtgggcgc cgggcgggcg agcgccgacc tcgaaggtgc
     2521 ggcccttgtc cgggcgctcg ccctgctggc gcacgccgca gctgcgtcgg cccttctcct
     2581 tgatgatggc gcgctcgcaa gtcgcgggct cgtcgcccca cgggtgcagg gcgccgtcgg
     2641 gtccgcgggc ggctttttcc cactgcgcct cggtcgggag atgcttgccc tgggcagtac
     2701 agtaggcgat cgcgtcgtac cagctgacgc cgacgatcgg ctggcgcggg cggtcgtaat
     2761 cgtcgtagcg gggaccggcg ggcgggcagt ttttggaaga ttcacaggcc ttgtattgcg
     2821 cgtgggtcac ctcgtatata tcgacgtaat aggtttgtag ccacacggtc gcgcgcgggc
     2881 gggtgttctc ggggccgtcc tcacgggggt cgctgccgcg gatgaacggg ccgccgggga
     2941 tgcaggcgag gccgggaggt ggcgggtcga ggcagggcag ggcgccgtcg ggggaggggc
     3001 tggcttttgg gacaggtgcc ggcggggccg ggggttgcac tggcggtggt gcgggcggtg
     3061 gtgcgggcgg tgccggctgc gccgcggcga ccggcgccgg tgggcggtcg caggcgaggg
     3121 cgaggcaagc acatgcgagc gcgaggcgca tcgtccgggc ttgtagcacg ggcaggggcg
     3181 caggacatga ccgacatgtc acgcttcgcc gtacgcccga tatgggtgga taccccgggt
     3241 atttattaaa tgtatttgcg ccatcacgcg gcgcgcatac cgtgatagcc tgcaaatgga
     3301 cgtgtccatt aaccttgtac ttgaggacgc aggacgatga cgaacattat gatcgtttcg
     3361 caattactcg tgctgtggag cgggcaggag gtgcccgggc cggcggccga tccgtctgag
     3421 gtgctcgcgc cgatcgagca ggcgcccgtg ccggtggtcg agcccaagcc gtcgatgcgt
     3481 gtcgtgccgg tggcagcgcc cgcagtcgtg ctgcaaaacg acgcgagcgc cgcgggagac
     3541 tggtcgcgtc tgcgtgggcg ccggcggttc ggcaggcggc tggcgggggg cgggttcgcg
     3601 gggttcggcg cggcgtacgt cggcaacttc atcgccggcg tcagatccgg cgaggtcggt
     3661 gaggatccga cgacgatggc gggtctgttc ccggtgatcg ggccgttcct gctggcgggc
     3721 tggaccggcc cgaacacgcc cgggttgaag acgttcttcg tgctcgacgg ggtggcgcag
     3781 gtcggctttc tcgcgatggg gatcacgggc gtggtgatcg ccaagcgctc ccgtcgtgcg
     3841 ctggcgcagc cgcgtttttt cgggctcaac ggtcagttcg agcatcgact cgacaatgac
     3901 cccgatctgc gccggcgcgg gcgtctgggg ctcggtctgg cgctcggcgg attcattggg
     3961 ttcggggtca cgtacctcag tacggtgatg acgatgctcg ggcccaagga cgagggcctg
     4021 ctcgccaacg agccgaatgt gcagcgcccg tgggtgatcg cgccgctcat cggcccgttc
     4081 gcgatcgcgg gtcaagcgga ggcggcgggt acgaaggcgt tgatggcggt cctcgggttg
     4141 gtccaggtcg gctcgctggc gacgggcatc accggcgccg tgatccgcgt gcgccgcgtg
     4201 cgggacgcgg agtcgcgagt tttgaagaac gtgtcgatcg cgccgtggca cagcgggacg
     4261 accaccgggc tgtcgctgcg cgggcagttt tgaggggcca gctttgtgtc acacgtaccg
     4321 gtttccgcgg tgggcgacga tgtcgtcgtc gtccgcgacc cggtcgacgg gcgctgggac
     4381 gtgctccggt cactcgccgt gccggcacgt gcggccgtcg ccgcaacccg cgagcagcag
     4441 cggcaccgtc agcaggatcg accgtgtcac gcctgtaccg cctccctgcg accgagcagc
     4501 gcgtcggcga tcgggcgcac gacgtcgcgg acgaccgcgc ggtggatcgc cgcccgcgcg
     4561 gccgccgaca gccgcccgtg cgcccgcaga tccggcccgc cgaccatagc gagcggggcc
     4621 gtcagcccgg ccgcggcgcg ccgcagcggc gccgcgacga gctcgccgta ggtgtcgacc
     4681 agccagcgca gcgtctgcca cgcgagcgcc gcgtgctgcc cctcctcgcg ggcgatgtcc
     4741 cgcagcagcg ccgcgacctc cgtgtccctg cactcctcgg ccgcgagcgc cgcctgggcc
     4801 gcggacacgc tctcgccgac gcagccctcc tcgaacaccg cgacggcgat cgcggccggg
     4861 tcgccggcgc gggccaggct cgcggcgtcg acgaccagcg gccccggccc gcatggcgcc
     4921 ccgcggtacc ggctcgcctg cgcgaacgac cgcgccgcgt gctcgacctc gtcggcgagc
     4981 gccgcctgcg tcgcgcgcag cagctccgcc ggcgccccga gggacatcag ctcgagcacc
     5041 gcgcgggcga acgacgcgat cgaggcgtgc tcggccagcg ccgcctgcgt ccacccctcc
     5101 gcgagcgccg cccgcgtcgc cgcgggcagc acctccgctg tcctgtcttc cgcgccccaa
     5161 tcgccgcgcc gcacgaccgc tgccgtgcgc gcgacgtcct cgacgatgaa cggccgcccc
     5221 tcgtagggga cgcaggtgtt cccgaggtgg cggtgcttgc cgggctcacc gagggcgacg
     5281 agcccgtact cactctcgcc gtcctcgatc acgacgcgca gcccgggccc gagcgtgtag
     5341 atcgcaccgc tcggcgcgtc ctcgtaggcc tcgaactcga agacccgccc gtccgccgtc
     5401 agcaccaccg ggtgcacgtc gagcacgtcg ctgtcggtcc cgaggtcgaa cttgtgcagc
     5461 gtcccgtcgt ctccgaggcg gagcagcgtg ccgttcgtcc cgccgatcag gccgaggcgg
     5521 acgatcgtca ggtcctcgct ggtcccgctg tcctcgcgct gccacgtccc gttcggatcg
     5581 ctcgcgcgcc agatcgctcc gccgcgcccg accgcgacga tcgtcccggt gtacggctgg
     5641 gcgtcgacgc cacgcagcga gccccacccg cccttcggcg ccgggacggc cgaccacgcc
     5701 ccggtctccg cagcacgggc gaacatcacc ccctcgccga cgacgacgac gatctcctcg
     5761 cccgcttcgg tctcgaaggt cgtcaccgcg tggaggtcgg ccgtcgtgcc gaggtcgatg
     5821 acctgccact cgtcgctgcc gcgcgacgcc acgaccaccg tgccgccgtc gccgaccgcg
     5881 acgacgagct cccgcgtgat cgtcaccccg cgcaggtcgg cggtgaccgg tcgatgtatg
     5941 cgcggcgtgg aagtcacgtc ctcgacgatc accccaccgc tgccgacgag gacctgcgac
     6001 ccgtcgtacg tcgaccacac gtcgccgagg atcgtcgcct cgacggggag atcgaacgcc
     6061 tccggggtga gccacgggtc gcggcagccg gtgctgcacc ccggcagggg cacgagggcc
     6121 gcggccgcgc cggccgcgag gagcatctgg atcacgagca ccgaacgcag ccgcaacatg
     6181 aagactccga tctgcaacga accggatgaa catacgcccg cgctcacatg tcgtcgagcc
     6241 tggcggatct cggcgcggtc cagcgccctc ggaggcccgg aaatttttac agcgagtcga
     6301 acacgcgccg gagcgcctga cggccggcgg cggcgaattg caggtcggcg tgctcgtcgg
     6361 cccacgagac ggtcgccatc gcgtgcatcg ccgcgagcac gctcagctgg cgctgctctt
     6421 gtgggttcaa cgtacgaccg tagccggcga agaacgcggc ctcgaggtcg ggtcgcgtgc
     6481 cccagacctc cgtccacagc ttgaccatgt cgagcagcgg cgtgtcgagg tgggcgtgct
     6541 cgaagtcgat cacgaagaag ctgtcgaggg tgaggatcgg gccgtcggag tcgctgccgg
     6601 agaccagcca gttgccgggc gtgaagtcgc ggtggcaggg cacgcggcgg gccccggcga
     6661 acagccgcgg gtcgtcggcg agggccgtga aggcggcgag gcgggcgtgc gtggtcgcgt
     6721 cgacggtcgg tcgcacgcgg gcgagccagg ccgcgtagcg ctgcgagatc gcagcggcga
     6781 gggggagcgg gtcgcggtcg ggctcgcgca ggtcgtgtag cgcgcgcagg aaccggccgg
     6841 cgcgctcgtg ggcggcgcgc tcgctgtccg gcgtggcgac ggcgccagcg agcgggcgac
     6901 cgggcacgac gccgaggacc agcgcgcgca ccggggccgc gaaggcggcg agcaacgggg
     6961 ctgtctcttc ggacaggtgc gggagccagc gggcgtaggc gtggcgctcc tggtggaagg
     7021 cgcggtcgtt gcggaattgt ttgacgacga cgccggggcc gggctcgctg ccgctgactt
     7081 gccagacgcg gcaggggccg agggcggagc cgatgaactg ccagcgcagg gcgtggctgt
     7141 cgcagccgac gtggtcgcag acgccggcga gcaggctggc gtcggcgtcg ctagtctgac
     7201 caggatgacc aggatgccca ggcggcggtg gggcctgcac ggtcacctgc gggcccgcag
     7261 cgcgccgagg gacacgcggg agtcgccgcg caggtaccac agcaggccgc cggcgagcac
     7321 gagcagcgag acgaggccgc aggcgaggcc ccaggcccag tacggtccgc cgaggcgcag
     7381 cgacatctgg gcgacgtcgg aggcgagctc gccggcgctc gagtgggcga cctcggtgaa
     7441 caggtagtcg ctgcgggtga acacgctgag cgccatctgc acggcgagga acaccaggag
     7501 gatctgggcc caggcggcgg aggtgcagaa ggcggcgacg agcgagaggc cgccgacgac
     7561 cccgaggtag ccctgggtga agccggggtc gccgacgagc accgcagcgg cgatcagggc
     7621 cgcgccgaac acggcgaggg cgaggcgggc gagcgcgggg cggcgggcga gcacgaacca
     7681 cacggccgcg acgatcgccg ggccgaccag gccgccggcg cacacgaggg cgtgcgcgag
     7741 gtcggtgccg gtgccggcgg tgaaggcggt gcccgagccg tcggggcgga tgatcaagcg
     7801 gtcgaagcta ccgccaaggg cgaccgcggt caggccgtgg ccgagctcgt gggcgacggt
     7861 cgacagccac accagtggga tgccgagcac gctcagcagc gggatcctcg gcagcaggag
     7921 cgtcagcccg acgctccaca gcagggcccg gcgcgcctgg cgggtgaccg tcacgcggcc
     7981 gatctctcgc tgatgcgctc gcgcggcggc cgacatgccg tcgatggtac cacgcgtcct
     8041 cccatgggcc agggatgcgc cgttcaggcg accgctggca cgccgaagcg ctcgcgcagg
     8101 gcggcgatcg gctggtccat gatgtcccag aagtcgtgct tcgcggagaa gtcgacggcg
     8161 gtgccgcggc cgcggtcgag cgcgcgcagg atcttgtcgt ggtcgaagag gacgcgggcg
     8221 ccggcgagct ggccgccggg ggcgaccttg cggcctgcgg tgtactgcag caggaccagg
     8281 aacagggcgc tgaagggatc ttcctggcgc atgccggcga cgaaggcgag ggtctgcagc
     8341 tcgccctcga ggctgctgtc gtagcccgag atgatgtgca tgatgtcgtg ctcgacgagc
     8401 gcgtcgggca cggtgccggg gtcgctggcg agctcgaact ctcgctcctt gtagtagcgc
     8461 cagagttcgc ggccgagcgt gccctcgtcg aggctgccga gctggcggta gcgctgcgag
     8521 acctcggcgt tctcgcgggc ggcgccgtgc gaggacggga tcgacagggt cgagggggtc
     8581 gagggcgagg cggtcgggcc ggaggcggcg gcggcgagca cgtcggcggg gaagaattgc
     8641 atcacgccct gccagccctc gctggcgtac agggtgccga gctgcggcgc ggcgaggcgg
     8701 cgctggtact cgtgcatcgt cagctggcgg tggcgatgta cgagctcgtg gaagctgtgc
     8761 agctgggcct cgtcgatcgc gagggcggcg cggaactccg acagcacctt ccactcctcg
     8821 ggtgagatct ggccgtcgac gatgcccatc accaggcagg ccttgagcgc gacgacgcgg
     8881 gacgcgaggc tgccgacctc gatggcg
//
LOCUS       DASWNJ010001723         1605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_135588, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001723 DASWNJ010000000
VERSION     DASWNJ010001723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135588"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="VGB85_28630"
     CDS             complement(<1..1000)
                     /locus_tag="VGB85_28630"
                     /inference="COORDINATES: protein motif:HMM:NF020932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HEY0138099.1"
                     /translation="MTRLVRPVLCALSLGTATILALAGACAEDSARQDVLTHLAEGVI
                     VPAQDRLRADADALASALGMHCSSGGGTVGPAWTAVRARWSFTGAFAFGPVATQMQAG
                     PLDFWPVRPDTIEEKIAAAPATIDAAYIDGLGTSAKGMPALEYLLFPAPPDPNTPRCA
                     YALALAQDIARRTAAIADAWHTEHADALATAGHGSTIYTSEQAGLDAVVNATIENLYR
                     MVKDKLDRPLGNLTGSAPDPTLVESYYSGGALADLDANLDGFAAIYLGADLQLGADLQ
                     AAGDPGLGELIAARDPGLDDRIITQLGVARAALAAIPSPLGEALTSDRGAVQSARDE"
     gene            complement(997..>1605)
                     /locus_tag="VGB85_28635"
     CDS             complement(997..>1605)
                     /locus_tag="VGB85_28635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEY0138100.1"
                     /translation="NVRQQTAGAFLGDMGISSSLFPLENCPEGQDACRAAASGGSPEI
                     DDDLLADVALYSSTLAVPARRDPEAPEVLRGKQLFGAAGCGDCHTPRHVTGDMPDLPE
                     VAGQTIFPFTDLLLHDMGEGLADHRPDYDASGTEWRTPPLWGLGLVATVNGHTEFLHD
                     GRARSLLEAVLWHGGEAEASRDAVLTMTADERAALLAFLGSL"
BASE COUNT          188 a          517 c          679 g          221 t
ORIGIN      
        1 tctcgtcgcg ggcgctttgc acggcgccgc ggtcgctggt cagggcctcg ccgagcggcg
       61 acgggatcgc ggcgagggcg gcccgggcga cgccgagctg agtgatgatg cgatcgtcga
      121 ggccggggtc gcgggccgcg atcagctcgc cgaggccggg gtcaccggcg gcctggaggt
      181 cggcgccgag ctggaggtcg gcgccgaggt agatcgcggc gaagccgtcg aggttggcgt
      241 cgaggtcggc cagcgcgccg ccgctgtagt acgactcgac gagcgtcggg tcgggggcgc
      301 tgccagtgag gttgccgagc gggcggtcga gcttgtcctt gaccatgcgg tagaggttct
      361 cgatcgtcgc gttgacgacg gcgtcgaggc cggcctgctc gctggtgtag atcgtgctgc
      421 cgtggccggc ggtcgccagc gcgtcggcgt gttccgtgtg ccaggcgtcg gcgatcgccg
      481 cggtgcgcct tgcgatgtcc tgcgcgagcg ccagcgcgta ggcgcagcgc ggggtgttcg
      541 ggtccggcgg cgcggggaac agcaggtact cgagggcggg catgcccttg gcgctggtgc
      601 cgaggccgtc gatgtaggcg gcgtcgatcg tcgccggggc ggcggcgatc ttctcctcga
      661 tcgtgtcggg gcggaccggc cagaagtcga gcgggccggc ctgcatctgc gtggcgaccg
      721 ggccgaaggc gaacgcgccg gtgaagctcc agcgggcccg cacggcggtc caggccgggc
      781 cgacggtgcc gccgccgctg ctgcagtgca tcccgagcgc gctcgccagc gcgtcggcgt
      841 cggcgcgcag ccggtcctgc gccggcacga tcacgccctc ggcgaggtgc gtgaggacgt
      901 cctggcgcgc gctgtcctcg gcgcaggcgc cggcgagcgc gaggatcgtg gctgtcccga
      961 gggacagggc gcacaggaca ggtcggacca ggcgcgtcac agggagccca ggaaggcgag
     1021 cagggcggcg cgctcgtcgg cggtcatcgt cagcacggcg tcgcgtgacg cctcggcctc
     1081 gccgccgtgc cacagcacgg cctcgagcag cgagcgggcg cgaccgtcgt gcaggaactc
     1141 ggtgtggccg ttgacggtgg cgacgaggcc gaggccccac agcggcgggg tgcgccactc
     1201 ggtgccgctg gcgtcgtagt cggggcggtg atcggcgagg ccctcgccca tgtcgtgcag
     1261 caggaggtcg gtgaacggga agatcgtctg gccggcgacc tcgggcaggt cgggcatgtc
     1321 gccggtgacg tggcgcgggg tgtgacagtc gccgcagccg gcggcgccga acagctgctt
     1381 gccgcgcagg acctcgggtg cctccgggtc gcggcgcgcg ggcacggcga gggtgctcga
     1441 gtagagggcg acgtcggcga gcaggtcgtc gtcgatctcg ggcgagccgc cgctcgccgc
     1501 ggcgcggcag gcatcctgtc cttcgggaca gttctcgagc gggaacagcg aggagctgat
     1561 gcccatgtcg ccgaggaagg cgccggcggt ctgctggcgc acgtt
//
LOCUS       DASWNJ010001724         1832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_209106, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001724 DASWNJ010000000
VERSION     DASWNJ010001724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209106"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1549
                     /locus_tag="VGB85_28640"
     CDS             <1..1549
                     /locus_tag="VGB85_28640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138101.1"
                     /translation="ELRSADQVLVDGAPAQALAGALSRFRFDSAPLIRLPRDPAALGQ
                     SWSWAAGPSTATANIGGIIGGNVLREFAVRFTHRVEAEATRFEITFYREFPGSEAVLA
                     DQGRAAIPLQFPGLLLGKDITDVCLVGGESCDFMSTFDRERVASALRPSRMVLDACVS
                     PPPATVEWDPDRKRCRLAPGPDAPAGQYHSATGASGTRAEEDRSCVVDPPPLDTTDLD
                     RGHEASLVVATGVPGLILFEDSARRLLNDLKLPPCKPDIDDIGGTEFTAPACLDGELD
                     TLALPGWPAAGSADRPLRRVRVRSVAFVPGLATSTGASACQRLEHRQKALKAQCDSAA
                     AGNGPRLSSDNVCAVAVQETAAVVGEAFVLEGLTGTPGPNPARWISTIVVPADHPMVQ
                     ALRRDVAPEALQADGMIGTALFNNSEVVLDYTDGTPGIRVSCSEPDAGTCVALPQCVP
                     GAPREPGEASDTTPACCYGLPEDLLISLIRDSGEYACCAALSSATVAELNLQAESEGR
                     EPPCIAR"
BASE COUNT          241 a          735 c          605 g          251 t
ORIGIN      
        1 cgagctgcgg tccgccgatc aggtgctcgt cgacggggcg cccgcgcagg ccctcgcggg
       61 cgcgctgagc cgctttcgct tcgactccgc cccgttgatc cggctcccgc gcgacccggc
      121 ggcgctgggc cagagctggt cctgggccgc gggtccgtcg acggcgaccg ccaacatcgg
      181 cggcatcatc ggcggcaatg tgctccgcga gttcgccgtc cgcttcacgc accgcgtcga
      241 ggccgaggcg acgcgcttcg agatcacgtt ttaccgcgag ttccccggct cggaggccgt
      301 gctcgccgac cagggccgtg cagcgatccc cttgcagttc cccggcctgc tgctcggcaa
      361 ggacatcacc gacgtgtgcc tcgtcggcgg cgagagctgt gacttcatga gcaccttcga
      421 tcgcgagcga gtcgccagcg ccctgcgtcc gagccgcatg gtgctcgacg cctgcgtcag
      481 tccgccgccc gcgaccgtcg agtgggaccc cgatcgcaag cgctgccgcc tcgcccccgg
      541 ccccgacgcg cccgccggtc aatatcactc ggcgaccggc gcctccggca cgcgcgccga
      601 ggaggaccgc agctgcgtcg tcgatccacc gccgctcgac accaccgacc tcgaccgcgg
      661 ccacgaggcc agcctggtgg tcgccaccgg cgtgcccggc ctgatcctct tcgaggacag
      721 cgcgcgtcgc ctgctcaacg acctcaagct gccgccgtgc aagcccgaca tcgacgacat
      781 cggcggcacc gagttcaccg cgcccgcctg cctcgacggc gagctcgaca ccctcgccct
      841 gcccggctgg cccgcggccg gcagcgccga tagaccactg cgcagggtcc gggtgcgcag
      901 cgtcgccttc gtccccggcc tcgcgacctc gaccggcgcc tccgcgtgcc agcggctcga
      961 gcaccggcaa aaggcgctca aggcccagtg tgacagcgcc gccgccggca acggcccgcg
     1021 cctctcctcc gacaacgtct gcgccgtcgc ggtccaggag accgccgccg tcgtcggcga
     1081 ggccttcgtg ctcgagggtc tcacgggcac tcccggtccg aaccccgccc gctggatctc
     1141 gacgatcgtc gtccccgccg atcacccgat ggtccaggcg ttgcgccgcg acgtcgcccc
     1201 cgaggcgctg caggccgatg gcatgatcgg cacggccctg ttcaacaact ccgaggtcgt
     1261 cctcgactac accgacggca cccccggcat ccgcgtgtcg tgcagcgagc cggacgccgg
     1321 cacctgcgtg gccctcccgc agtgcgtgcc cggtgcgcca cgcgagccgg gcgaggccag
     1381 cgacaccacg cccgcgtgct gctacggctt gcccgaggac ctgctgatct cgctgatccg
     1441 cgacagcggc gagtatgcct gctgcgcggc gctctcgtcc gcgacggtcg ccgagctcaa
     1501 cctgcaagcc gagagcgagg gccgcgagcc gccctgcatc gcccgctgac ctgccgcgcg
     1561 agcggtccag cgccggagca gagcgggtca cgatcgcggg cgtgcccgac atgcccctgc
     1621 ggcagtgtgc gcgacaacgt ccggcgcccg cctcgcatca cccgcgcgcg atgtcatcgc
     1681 ctccggtcgt tccacgtagc gacgctcgac cgcaccagga cgacctctcg cctgccccgc
     1741 cgccatgacc gggggtatgc aggtcggtcg gagagatacc atgcgacgac gacttggttg
     1801 ggcgggacgc gtgtccctgg cgtggatcag cg
//
LOCUS       DASWNJ010001725         5699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_596231, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001725 DASWNJ010000000
VERSION     DASWNJ010001725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596231"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..876)
                     /locus_tag="VGB85_28645"
     CDS             complement(103..876)
                     /locus_tag="VGB85_28645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138102.1"
                     /translation="MFSTITLLASTLAFNLTATPAQTETKPQDKPGKGLFGLDIDLGG
                     DVDVELGDCLGVDIEVGAKCGVLAGAAAALECDPLSVEAACIAELGADCGVEAFAACT
                     AELTAHCEAEVQAGGALFCDGNFIGADTCLGDIDVDIDIDTKDCVDVDLGDDVDLNID
                     LDASVCLDLEIGLNAECYIKVGAACHAACDPVAVEAACEGELDGSDDELHAYATCQAG
                     LIAQCHANCDADGSLFCDGTIYAGADLCLDIGLGIGIGF"
     gene            complement(1440..2894)
                     /locus_tag="VGB85_28650"
     CDS             complement(1440..2894)
                     /locus_tag="VGB85_28650"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0138103.1"
                     /translation="MRRPGLLVVLLVGSSCSVSNPLFAVDQDDPIATEGPGPTTQGAT
                     SEATSEPGTTTDEPGTGSATQTGDATTDEPDPVCGDGDLDEASEECDDGNQIETDTCL
                     PGCVAARCGDGIVHTNHEACDDGNGDDKDECTNSCALAGCGDGVKQPDEECDDGNKVD
                     DDVCSNTCLTAKCGDGITQKTEMCDDGNDVDDDECIDCVTAVCGDGVVQSGVEQCDDG
                     NDDDYDTCITECKDASCGDGFIQADEMCDDGAMNDETCPDCKLPPVCGDGIISGEESC
                     DYNAQPYKDVGLPVCTKSCQLLGCTLFKNTPEQDFDAVLADTWLTPCTWGAAKTVVVT
                     LLDETRQVVYMAKGPIGGDWTQTNLTAGEIAPTSEFNVTAHQRRVKLQRIMPKDVDID
                     VLMLSSQDANPGNDQPACYKGLGDGYGLAIFSELLPPQEPKLLVMGVLGGVTNNKRFP
                     MSDGSREISYDDGGMDVCVDTTPFLGALLLSVFP"
     gene            complement(2935..4131)
                     /locus_tag="VGB85_28655"
     CDS             complement(2935..4131)
                     /locus_tag="VGB85_28655"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidogalycan biosysnthesis protein"
                     /protein_id="HEY0138104.1"
                     /translation="MDAAQWNALEHGPSPFSEHGFLRALERSGSVGADSGWDPAYILV
                     EAPAERSPSAGAASAQASGEIPGARLVGAVAAYVKAHSYGEYIFDWAWARASEQGGLR
                     YYPKLVVAVPMTPATGPRLLVARDVARGPVVEKLVAGVKALADRRRCGSIHWLFTTAE
                     EQVELRDHGFSPRASFQYHWHNAGYASFDEFLARLTSRRRKQLRKERARVHAAVPAVE
                     WVPGTALTSADTEIIDRFYRGTTHNHFGQDYLQPGFFQAVVEEQPERVQWARVRRGEQ
                     TIAGALYFETDAALYGRYWGCAEEVEFLHFEASYYAAIERCIARGTPLFEAGAQGEHK
                     LIRGFAPSATYSSHWIRHPGLAAGVERFLVDEAVAVREHMRQLATLLPFREDASELDA
                     KVTADP"
     gene            4250..4594
                     /gene="clpS"
                     /locus_tag="VGB85_28660"
     CDS             4250..4594
                     /gene="clpS"
                     /locus_tag="VGB85_28660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEY0138105.1"
                     /translation="MASREKNSPQGSVVLKTRSERKTTRPRMYRVLIHNDDYTPMEFV
                     VRLLQVVFHRNEAESTAIMLHIHNHGVGVAGVYTHEIAETKVAQVHMLARQQDCPLVA
                     SMEPEEDPDAEH"
     gene            4581..>5699
                     /locus_tag="VGB85_28665"
     CDS             4581..>5699
                     /locus_tag="VGB85_28665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEY0138106.1"
                     /translation="MLSTELKHTINAAIADTQRRRHEYITLEHLLLALLDDPSARKII
                     MACQGDLEKIRAELEAFIEANTERLPDEAEPTVHQTIGVGRVLQRAILHVQGTGRNEV
                     NGANLLIAIFAESESFAAHVLREHGVTRRDATMFMSHGIPKGGDKAPTSKPSANPIGD
                     GDDDEAVADDPLEAYAVDLNAKAAAGRIELLVGRDAEIQRVIQVLCRRRKNNPVLVGE
                     PGVGKTAVVEGLALRVHTGKVPAALASAHIYALDMGALLAGTRFRGQFEERLKAVIGA
                     IEDDPDAILFIDEIHTIVGAGATSGGTMDASNMLKPALASGELRCIGATTYKDFKSSF
                     ERDQALARRFQKIEVLEPSVAETIEILRGLQPAYEAHHG"
BASE COUNT          892 a         1984 c         1935 g          888 t
ORIGIN      
        1 attccgcggc gccgccccgc gcgggtccgg ggacacgcgg ggcgggaggc cgagggccgt
       61 caggccccgt cgtcgctggg ccgtcaggcc cgttcactcg ttttagaatc cgatgccgat
      121 gccgaggccg atatcgaggc acaggtcggc gcccgcgtag atcgtgccgt cgcagaacag
      181 cgagccgtcg gcgtcgcagt tggcgtggca ctgggcgatc aggccggcct ggcaggtcgc
      241 gtaggcgtgc agctcgtcgt cgctgccgtc gagctcgccc tcgcacgccg cctcgaccgc
      301 gaccgggtcg cacgcggcgt ggcacgcggc gccgaccttg atgtagcact cggcgttgag
      361 gccgatctcg aggtcgaggc acacgctcgc gtcgaggtcg atgttgaggt cgacgtcgtc
      421 gccgaggtcg acgtcgacgc agtccttggt gtcgatgtcg atgtcgacgt cgatgtcgcc
      481 gaggcaggtg tcggcgccga tgaagttgcc gtcgcagaac agcgcgccgc cggcctggac
      541 ctccgcctca cagtgggcgg tcagctcggc cgtacaggcg gcgaaggcct cgacgccgca
      601 gtcggcgccc agctcggcga tgcaggcggc ctcgacgctg agcgggtcgc actcgagggc
      661 cgccgcggcg ccggccagga cgccgcactt ggcaccgacc tcgatgtcga cgccgaggca
      721 gtcgccgagc tcgacatcga cgtcgccgcc gaggtcgatg tcgaggccga acagcccctt
      781 gccgggcttg tcctgcggct tggtctcggt ctgcgccggg gtcgcggtca ggttgaaggc
      841 gagggtggag gccaggagcg tgatcgtgga gaacatggga agctgccttg tcttgggtcg
      901 ccttcgcaga ggtcgcgtcg gcggatgagc tgaatatgtg gggcgctggt cggacggcaa
      961 tgcgacagcg ggccgccacc ctcgccagac gtccagcgaa ttgtcagaac agcctcgaaa
     1021 ccgcctcagg acgcgagatg cggggaaacg tgcgatcgag gccatggccg tcatcgcgcg
     1081 cgtcacgacg atcctgccac ccgtgcaaat ccaatgtgtg gtgataacac cgcagacgca
     1141 tccgcctcaa cgcaacattc ccggcttcgg acggctgtcc gtcgatgcgc gaacgcgacc
     1201 gctgtcggcg ttcaagtcat cgaaactgtt catgaataca ggcgttgggg gtggcgctac
     1261 ccgcgagttg aagcgccgtc ggggcaaggt ctgtcgaaga aaatcgacag tgtcgcggcc
     1321 tgcgtgtatg agcagacccg tcgcattgtt cgaattgccg ggtaggcgcc cacgagcgcg
     1381 gcggcgaccg ggtcacatcg ccgacaaacg cggcaaagct aggcggtctc caggcgactc
     1441 taaggaaaca cgctcagcag gagcgcgccc aggaacggcg tggtgtcgac gcagacatcc
     1501 atcccgccgt cgtcgtagct gatctccctg ctgccgtcgg acatggggaa gcgcttgttg
     1561 ttggtcacgc ctccgagcac gcccatcacc agcagcttcg gctcctgcgg cggcagcaac
     1621 tccgagaaga tggccaggcc ataaccatcc ccgagcccct tgtagcaggc cggctggtca
     1681 ttgcccgggt tggcgtcctg gctggacaac atcagcacgt cgatgtcgac gtccttgggc
     1741 atgatgcgct ggagcttgac ccgtcgctgg tgagcggtga cattgaactc gctggtgggc
     1801 gcgatctcac cggcggtcag gttggtctgc gtccaatctc cgccgatcgg gcccttcgcc
     1861 atgtagacca cctgcctggt ctcgtcgagc agcgtgacga ccacggtctt cgccgcgccc
     1921 cacgtgcacg gcgtgagcca ggtgtcggcc agcacggcgt cgaagtcctg ttccggggtg
     1981 ttcttgaaga gagtgcagcc caggagctga cagctcttcg tgcacaccgg gaggccgaca
     2041 tccttgtagg gctgggcgtt gtagtcgcaa ctctcttcac ccgagatgat cccgtccccg
     2101 cagacggggg gcagcttgca gtccgggcac gtctcgtcgt tcatcgcccc gtcgtcgcac
     2161 atctcgtccg cctgtatgaa gccgtcgccg cagctcgcat ccttgcactc tgtaatgcag
     2221 gtgtcgtaat cgtcgtcgtt gccgtcgtcg cactgctcca cgccggactg cacgacgccg
     2281 tcgccgcaca ccgccgtcac gcagtcgata cactcgtcat cgtcgacgtc attgccgtcg
     2341 tcacacatct cggtcttttg cgtgatgccg tcgccgcact tcgccgtcag gcaggtgttg
     2401 ctgcacacgt cgtcatcgac cttgttgccg tcgtcgcact cctcgtccgg ttgcttgacg
     2461 ccgtcgccgc agcccgcgag cgcgcagctg ttggtgcact cgtccttgtc gtcgccgttg
     2521 ccgtcgtcgc aggcctcgtg gttcgtgtgc acgatgccgt cgccgcaccg cgccgccacg
     2581 cagccgggca agcacgtgtc ggtctcgatc tggttgccgt cgtcgcactc ctccgacgcc
     2641 tcgtcgaggt caccgtcgcc gcacaccggg tccggctcgt ccgtcgtcgc atcgccggtc
     2701 tgcgtggcgc tgcccgtgcc cggctcgtcg gtcgtcgtgc cgggctcgct cgtcgcctca
     2761 ctcgtcgcgc cctgcgtcgt cgggccggga ccttcggtcg cgatcgggtc gtcctggtcg
     2821 acggcgaaca gcgggttcga caccgaacag gaggacccga cgagcaacac gacgaggagg
     2881 ccagggcgac gcatcggacc acgatatccg ccggcgtcgc tggcgtcgac gctgctaggg
     2941 gtcggccgtc acttttgcat caagctcgga ggcgtcctca cgaaacggca gcagcgtggc
     3001 gagctggcgc atatgttcgc gcaccgcgac ggcctcgtcg acgaggaagc gctcgactcc
     3061 ggcggcgagc ccggggtggc ggatccagtg gctgctgtac gtggccgacg gagcgaagcc
     3121 gcggatcagc ttgtgctcgc cctgcgcccc ggcctcgaag aggggggtcc cgcgcgcgat
     3181 gcagcgctcg atcgcggcgt agtacgaggc ctcgaagtgg aggaactcga cctcctcggc
     3241 gcagccccag tagcgcccgt acagcgccgc gtcggtctcg aagtacagcg ccccggcgat
     3301 cgtctgctcg ccccggcgca cgcgcgccca ctggacccgc tccggctgct cctcgacgac
     3361 cgcctggaag aagccgggct gcaggtagtc ctggccgaag tggttgtggg tggtgccgcg
     3421 gtagaagcgg tcgatgatct cggtgtccgc ggacgtcagc gcggtaccgg ggacccactc
     3481 gaccgccggc accgccgcgt gcacgcgcgc tcgctccttg cgcagttgct tgcggcgccg
     3541 cgaggtcaaa cgcgcgagga actcgtcgaa gctcgcgtag ccggcgttgt gccagtgata
     3601 ctgaaagctc gcgcgcggcg agaagccgtg gtcacgcagc tcgacctgct cctccgcggt
     3661 ggtgaacagc cagtggatcg acccgcagcg gcggcggtcg gcgagcgcct tgacccccgc
     3721 gacgagcttc tcgaccacgg gaccgcgcgc gacgtcgcgg gcgacgagca ggcgcgggcc
     3781 ggtcgccggc gtcatcggca ccgcgacgac gagcttgggg tagtagcgca ggccgccctg
     3841 ctcggacgcc cgcgcccagg cccagtcgaa gatgtactcg ccgtagctgt gggccttcac
     3901 gtaggccgcg acggcgccga cgagccgcgc gcctgggatc tccccggacg cctgcgcgct
     3961 cgctgcgccc gccgacgggg acctctccgc gggggcctcg acgaggatgt acgcggggtc
     4021 ccagcccgag tcggcgccga ccgagccgga gcgctcgagg gcgcggagaa agccgtgctc
     4081 gctgaaggga gaggggccgt gctcgagcgc gttccactgt gcggcgtcca cgctggcgag
     4141 cgatgacacg acgcggcttg tcagatccgg caccgcctac gtttacgctg ggtccgggcg
     4201 cctgaccagg gtgcgctata gtaccttcag gcagcgcctc acggaagcaa tggccagtcg
     4261 cgagaagaac agcccccagg gcagcgtggt cctcaagacc cgcagcgagc gcaagaccac
     4321 ccggccgcgg atgtaccgcg tcctcatcca caacgacgac tacaccccga tggagttcgt
     4381 cgtgcgcctg ctgcaggtgg tgtttcaccg caacgaggcg gaatccacgg cgatcatgct
     4441 gcacattcac aaccatggcg tcggcgtcgc cggcgtctac acgcacgaga tcgcggagac
     4501 aaaggtcgcc caggtccaca tgctggctcg ccagcaggac tgcccgctcg tcgcctcgat
     4561 ggagcccgag gaggaccccg atgctgagca ctgaactcaa gcacacgatc aacgccgcga
     4621 tcgccgacac ccagcgccgc cggcacgaat acatcaccct cgagcacctg ctgctggcgc
     4681 tgctcgacga cccgagcgcc cgcaagatca tcatggcctg ccagggtgac ctcgagaaga
     4741 tccgcgcgga gctcgaggcc ttcatcgagg ccaacacgga gcgcctcccg gacgaggccg
     4801 agccgaccgt gcatcagacg atcggggtcg gtcgcgtgct ccagcgcgcg atcctccacg
     4861 tgcagggcac cggccgcaac gaggtcaacg gcgccaacct gctgatcgcg atcttcgccg
     4921 agagcgagtc gttcgccgcg cacgtgctcc gcgagcacgg cgtcacccgc cgcgacgcga
     4981 cgatgttcat gtcgcacggg atccccaagg gcggcgacaa ggcgccgacg agcaagccct
     5041 ccgccaaccc gatcggcgac ggcgacgacg acgaggcggt cgcggacgac ccgttggagg
     5101 cctacgcggt cgacctcaac gccaaggccg ccgccggccg catcgagctg ctcgtcggcc
     5161 gcgacgcgga gatccagcgc gtcatccagg tgttgtgtcg ccgccgcaag aacaacccgg
     5221 tgctcgtcgg tgagcccggc gtcggcaaga cggccgtcgt cgagggcctg gccctgcgcg
     5281 tgcacaccgg caaggtcccg gccgcgctcg ccagcgcgca catctacgcg ctcgacatgg
     5341 gcgccctgct cgccggcacg cgctttcgcg gccagtttga agagcgcctc aaggcggtga
     5401 tcggcgcgat cgaggacgac ccggacgcga tcctcttcat cgacgagatc cacacgatcg
     5461 tcggcgccgg cgcgacctcc ggcggcacca tggacgccag caacatgctc aagccggcgc
     5521 tcgcgtccgg cgagctgcgc tgcatcggcg cgacgaccta caaggacttc aagtcctcgt
     5581 tcgagcgcga ccaggcgctc gcccgccgct tccagaagat cgaggtgctc gagccctcgg
     5641 tcgccgagac gatcgagatc ctccgcggcc tgcaacccgc ctacgaggcg caccacggc
//
LOCUS       DASWNJ010001726         3521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_563062, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001726 DASWNJ010000000
VERSION     DASWNJ010001726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563062"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VGB85_28670"
     CDS             complement(<1..827)
                     /locus_tag="VGB85_28670"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY0138107.1"
                     /translation="MRPSQIAAQLECNIKAGISTMLHGSPGVGKSDTVRQLAARLDWD
                     LRDIRISQMDSVDLRGVPSIRDGRTYWNVPSFLPEPGPKPGILFLDEINSAPQSTMAA
                     AYQLVLDRRLGDYVVPDNWAIIAAGNRQTDRAIVNQMSTALKNRFVHLTYDPNIEDWS
                     SWALQADIVPEVLAFLRFRPNLLNEFDAPVGNEDMKQKKRNMKDATAFATPRSWEFVS
                     RLVKANQPKDSEFESFSGAVGEGAAVELMAFMKHHRSLPDLDALLMNPKTTEVPEEPA
                     "
     gene            complement(878..1201)
                     /locus_tag="VGB85_28675"
     CDS             complement(878..1201)
                     /locus_tag="VGB85_28675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138108.1"
                     /translation="MTTPTADKASSTSDPHTDDKKKFALIAKLIERLSETPVSKIHGM
                     LVTLAFESPDAPEMVDIEHFMFGHHELLEGMLSSSIDSVLQVHAEHCPHCSEKLREAK
                     HATQH"
     gene            1365..1973
                     /locus_tag="VGB85_28680"
     CDS             1365..1973
                     /locus_tag="VGB85_28680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138109.1"
                     /translation="MAGLEKTLTDRVLTYLAKHPNSTTPQIAKALKTTSQQASTRCTQ
                     LWESGRLSREARDGDLKGGRFTYSLGDEDAPKTVTRAAKKAKAAARTAAAPSPTPPGD
                     KTPIPGEEKEEERQNEDHEAGDATAAYVAQGEFPGVGEIEPLAPRPGAALQGLVQALA
                     AALADEIMSHLQSELNTRVAALYRDAAARPPQAERRLRSVTN"
     gene            1973..2098
                     /locus_tag="VGB85_28685"
     CDS             1973..2098
                     /locus_tag="VGB85_28685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138110.1"
                     /translation="MWEVVLYVLGGVALLALGAVLYVIGLVIYGALKYPDDPGNP"
     gene            complement(2095..2556)
                     /locus_tag="VGB85_28690"
     CDS             complement(2095..2556)
                     /locus_tag="VGB85_28690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138111.1"
                     /translation="MILYQLNKVYQGEPQWHGTRDEARKAARQHPEIPLAGILISQYD
                     VPVDKEGVLGILNHKPGTAKLMRQWEFSNRGGLVELGIPDEDGTDQPALQVYDTLATV
                     EAKVKQAVTVAARLTEMREDDALPVDPQKQREVDEHRGRSTWHRQHPPKED"
     gene            complement(2553..2693)
                     /locus_tag="VGB85_28695"
     CDS             complement(2553..2693)
                     /locus_tag="VGB85_28695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138112.1"
                     /translation="MNEAEMLAEILQLIAPYPHFRQDPVWAKIYYLWIAEAAKTTPGV
                     KR"
     gene            complement(2690..2857)
                     /locus_tag="VGB85_28700"
     CDS             complement(2690..2857)
                     /locus_tag="VGB85_28700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138113.1"
                     /translation="MKWFGEPPLESDAELWYWVWQTLRMRDPDLLVRSHEDPMVGLAW
                     AKYAQLRDLPP"
     gene            complement(2854..3048)
                     /locus_tag="VGB85_28705"
     CDS             complement(2854..3048)
                     /locus_tag="VGB85_28705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138114.1"
                     /translation="MNNRDLADLQEQWLERLTFWAAGMTLHADSLFLHKDLRTGTDVE
                     KYRRDAAQHLKRFARHGLRP"
     gene            complement(3045..3182)
                     /locus_tag="VGB85_28710"
     CDS             complement(3045..3182)
                     /locus_tag="VGB85_28710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138115.1"
                     /translation="MTREQWFFHLMVKVRALDGCGYTPPRRYENYRRALINFLKMHPP
                     P"
     gene            complement(3179..>3521)
                     /locus_tag="VGB85_28715"
     CDS             complement(3179..>3521)
                     /locus_tag="VGB85_28715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138116.1"
                     /translation="RPGFQIRYEFDAPKKMPVINDKMIRFNGMGDDGHETMMLDREHD
                     PDFRFCKTARKDYDEAVLVLLTLAEHHAPGAWKISSDGEEFEWQPAVDWMNTLGMGDF
                     EVPPGVRQFEE"
BASE COUNT          557 a         1103 c         1198 g          663 t
ORIGIN      
        1 gccggctcct cgggcacctc ggtcgtcttc gggttcatga ggagcgcatc caggtcgggg
       61 agcgagcggt ggtgcttcat gaaggccatg agctcgactg ccgcgccctc gccgaccgca
      121 ccggagaagg actcgaattc gctgtccttc ggctggttcg ccttcaccag gcgcgagacg
      181 aactcccatg agcgcggcgt cgcgaacgcg gtcgcgtcct tcatgtttcg cttcttctgc
      241 ttcatgtcct cgttgcccac cggcgcatcg aattcattga gcaggttcgg ccgaaaacgc
      301 aggaacgcca gcacctccgg cacgatgtcg gcctgcagcg cccacgacga ccagtcctcg
      361 atgttcgggt cgtaggtgag gtgcacgaac cggttcttga gcgcggtcga catctggttg
      421 acgatggcgc ggtccgtctg tcggttgccg gccgcgatga tcgcccagtt gtccggcacg
      481 acgtagtcgc cgaggcgccg gtcgagcacc agctggtagg ccgcggccat ggtcgactgg
      541 ggcgcggagt tgatctcgtc gagaaagagg atcccgggct tcggtcccgg ctccggcagg
      601 aacgacggca cgttccagta ggtccggccg tcgcggatcg agggcacgcc tctgaggtcg
      661 accgagtcca tctggctgat ccggatgtcg cgcaggtccc agtcgaggcg cgccgcgagc
      721 tggcgaactg tgtccgactt tccgactcca ggtgagccgt ggagcatggt ggagatgccc
      781 gccttgatgt tgcattcgag ttgggccgcg atctgtgacg ggcgcatggg tagtgctcct
      841 ggtaaggatg gttgaggggt gaaacggggg gaaaacttca gtgctgcgtt gcgtgcttcg
      901 cttcgcgcaa tttttcgctg cagtgcgggc agtgctcggc gtggacctgc aagaccgagt
      961 cgatcgagga cgaaagcatg ccttcgagga gctcgtggtg gccgaacatg aagtgctcga
     1021 tgtcgaccat ttccggggcg tcgggcgact cgaacgccaa ggtcacgagc atgccgtgga
     1081 tcttcgatac gggtgtttca ctcaagcgct cgatcagctt ggcgatgagc gcgaatttct
     1141 tcttgtcgtc ggtgtggggg tcggaagttg aggaggcctt gtctgctgtg ggggtggtca
     1201 ttaagttttt tcctgggcta agggagagca gtatgagatt cgcatcagac acgtccacta
     1261 agtccgagga atttactcgg ttactccgcg agtgctttga cgcagttgct caaaaggcgc
     1321 aacctcgcga ttttcccccc taactgttaa atggagaagg acccttggcc ggactcgaaa
     1381 aaaccctcac cgatcgcgtg ctcacttatc tcgcgaaaca ccccaacagc acaacacctc
     1441 agatcgcgaa ggccttgaaa accacctcgc agcaggcctc cacgcgctgc acgcagctct
     1501 gggagtccgg gcgactctct cgcgaggccc gcgacggcga cctgaagggc ggacgtttca
     1561 cctattcgct cggcgacgag gacgcaccga agaccgtgac gcgcgcagcg aagaaagcga
     1621 aggcggccgc gaggaccgcc gccgcaccgt cccccacccc gccgggcgac aagacgccga
     1681 tcccgggcga ggagaaggaa gaggaacgac agaacgagga tcacgaggcc ggcgacgcca
     1741 ccgcggccta cgtcgcgcag ggcgaattcc cgggcgtggg cgagatcgag ccgctcgcac
     1801 cccgccctgg cgcggcgctg cagggcctgg tgcaggcgct ggcggcagcg ctcgccgacg
     1861 agatcatgtc gcacctgcag agtgagctca acacgcgggt ggcggcgctc tatcgcgacg
     1921 cagcggcgcg gccgccgcag gccgagcgcc gcttgcggtc ggtcacgaac tgatgtggga
     1981 ggtcgtgctc tacgtgctcg ggggcgtggc gctcctcgca ctcggggccg tcctctacgt
     2041 gatcgggttg gtgatctacg gagccctgaa gtaccccgac gaccccggca acccctagtc
     2101 ctctttcggc gggtgctggc ggtgccaggt gctgcgcccg cggtgctcgt cgacctcgcg
     2161 ctgcttctgc gggtcgaccg gcagcgcatc gtcctcgcgc atctcggtga gccgcgccgc
     2221 cacggtgacg gcctgcttca ccttcgcctc gacggtcgcc agggtgtcgt agacctggag
     2281 cgcgggctgg tccgtgccgt cctcgtcggg gatgccgagc tcgaccaggc cgccgcggtt
     2341 gctgaactcc cactggcgca tcagcttggc cgtgccaggc ttgtggttga ggatgccgag
     2401 cacgccctcc ttgtcgactg gcacgtcgta ctgcgagatc aggatgccgg ccaaagggat
     2461 ctcagggtgc tgcctggcgg ctttcctggc ctcgtcgcgg gtgccgtgcc actgcggctc
     2521 accctggtag accttgttga gctggtagag gatcatcgtt tcactcctgg tgtcgttttg
     2581 gcggcctctg cgatccagag gtagtagatc ttggcccaca ccgggtcctg gcggaagtgc
     2641 ggatagggcg cgatcagctg caggatctcg gcgagcatct cggcttcgtt catggcggca
     2701 ggtccctcag ctgtgcgtac ttggcccacg ccaggcccac catcgggtcc tcgtgtgagc
     2761 gcaccaggag atccgggtcg cgcatgcgca gcgtctgcca gacccagtac cagagctcgg
     2821 cgtcgctctc gagcgggggc tcgccgaacc atttcacggc cgcagcccgt ggcgggcgaa
     2881 gcgtttcagg tgttgggcag cgtcgcggcg gtacttctcc acgtccgtgc cggtccgcag
     2941 gtccttgtgc aggaacagcg agtccgcgtg cagcgtcatg ccggccgccc agaaggtgag
     3001 tcgttcgagc cactgctcct gcaggtcggc aaggtcgcgg ttgttcatgg cggcgggtgc
     3061 atcttgagga agttgatcag tgcgcggcgg tagttctcgt agcgccgcgg cggcgtgtag
     3121 ccgcagccgt cgagcgcgcg caccttgacc atcaggtgga agaaccactg ctcgcgggtc
     3181 attcctcgaa ctgcctgact cccggtggca cctcgaagtc gcccatcccg agggtgttca
     3241 tccagtcgac ggccggctgc cactcgaact cctcgccgtc ggacgagatc ttccaggcgc
     3301 cgggcgcgtg gtgctcggcg agcgtgagga ggacgagcac cgcctcgtcg tagtccttcc
     3361 gcgcggtctt gcagaagcgg aagtccgggt cgtgctcgcg atcgagcatc atcgtctcgt
     3421 ggccgtcgtc gcccatgccg ttgaagcgga tcatcttgtc gttgatcacc ggcatcttct
     3481 tcggggcgtc gaattcgtag cggatctgaa agcccgggcg g
//
LOCUS       DASWNJ010001727         3050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_524739, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001727 DASWNJ010000000
VERSION     DASWNJ010001727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524739"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1676
                     /locus_tag="VGB85_28720"
     CDS             <1..1676
                     /locus_tag="VGB85_28720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138117.1"
                     /translation="VESPKAPETPVIDPRRLPAEDPPGTGLWLAAGRDGDLRLVDLGG
                     ELVVAGAGVMARVGVDGSLEALPAMHHGMLEPEWMGVEVLMALGGRWPDPVWMTAQSQ
                     AHRSASAPFTYYRKGARWQRKATGVGPLDWYYQAFAPWTGEQTLALRLLTTDPNLESL
                     YGDDLPAKIRKQIDAAIRANPPRLDLLAAGTPPKPAPMQIAAGSRPIDLATLPTGEVF
                     VLLAFAENEGGEDERFAVQRFAPGEPAGVVDHLPEVGGAAPFMPRGKLVARAANDVYL
                     AGALRRNTGLEALVTRFDGETWRPITLPPGGQVLSLAFAADGVAWAVVERMDAEGHET
                     TGLWKRPGEGAWTEVTLPRVRTPALAEPRFLFDGDELLDWREAPGEPLRAAQPMAIEP
                     GQVAVHDGELWVLASVPEIGHGDQSRQVVLRSRPVQQILELTDSVSLGAEMSPGSLAT
                     SLDPKRGCNTGSTWVVVATIASGTDRDAGAAVAESFLASLPDELREGLVALRDFRVRG
                     RRVVAATMGFHDMEATMALAEATRRIRPDEKHPFECGNPRPIRSFHEHD"
     gene            complement(1679..2671)
                     /locus_tag="VGB85_28725"
     CDS             complement(1679..2671)
                     /locus_tag="VGB85_28725"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEY0138118.1"
                     /translation="MSRFVQRALFGVVLGVLLYVGAVLYVDIGRVRAALAGYAWSAVL
                     LALGLSSVNYLLRFLKWELCLGWLGVRGDGPEDAPGLTRGRSLLIYLAGLSMSVTPGK
                     VGEVLRSYLLRLTDGVPFSRTAPVVVADRLTDLVALVVLSLVGVSQFREYLPVVLITL
                     ALVVAGVVVLGSPRLCLGLLHSLSRLPGLGRLGGRAEALVHSSAALLRLRPLLVLSLI
                     SVVGWGLECIGYWLIVRGLVGAEASLALCTFLWSTTTLIGALSFLPGGLGATEGSLAV
                     LVARLATGVTQPAALATTLLIRACTLWYGELVGGIALAAVLRRAPPAPPPAPPA"
     gene            2802..>3050
                     /locus_tag="VGB85_28730"
     CDS             2802..>3050
                     /locus_tag="VGB85_28730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138119.1"
                     /translation="MSTSQGAVFWVGRVATPAGPRWVRVFTEGAKPAPEDPIEIVVDP
                     FADEHGDPYARVRASVRGEPVGTVGTAKLLPPVRPSKVV"
BASE COUNT          470 a         1012 c         1207 g          361 t
ORIGIN      
        1 cggtggagag cccaaaggca ccggagacac ctgtgatcga cccgcgccgg ctgccggcgg
       61 aagatcctcc cgggaccggc ttgtggctcg cggccggccg cgacggtgac ctgaggctcg
      121 tcgatctcgg cggagagctc gtggtcgcgg gggccggcgt gatggcacgt gtcggtgtgg
      181 atggatcgct cgaggcgctg ccggcgatgc atcacgggat gctcgagccg gagtggatgg
      241 gggtcgaggt gctgatggcg ctgggcgggc gctggccgga cccggtgtgg atgacggcgc
      301 agtcgcaggc gcatcgctcg gccagcgcgc cgttcacgta ctaccgcaag ggcgcgcgat
      361 ggcagcgcaa ggcgaccggg gtcgggcccc tcgactggta ctatcaggcg ttcgcgccgt
      421 ggaccggcga gcagacgctg gcgctgcggc tgctgacgac cgaccccaat ctggagtcgc
      481 tgtacgggga cgacctcccg gcgaaaattc gcaagcagat cgacgcggcg atccgggcca
      541 accctcctcg cctcgacctc ctcgcggctg gcacaccgcc gaagccggcg ccgatgcaga
      601 tcgccgcggg cagtcggccg atcgacctgg cgacgctacc gaccggcgag gtgttcgtgt
      661 tgctggcctt cgccgaaaat gaggggggtg aggacgagcg cttcgcggtc cagcgcttcg
      721 cgcccggtga gcccgcgggg gtcgtcgatc acctgccgga ggtcggcgga gcggcgccgt
      781 tcatgccccg gggcaagctg gtcgcccgcg cggcgaacga tgtctacctc gcgggtgcgc
      841 tgcgcaggaa cacggggctg gaggcgctcg tcactcgctt cgacggcgag acctggaggc
      901 cgatcacgct gccgccgggg gggcaggtgc tgtcgctggc gttcgccgcc gatggcgtcg
      961 cgtgggcggt ggtcgagcgc atggacgccg aggggcacga gacgacgggg ctgtggaagc
     1021 ggcccggcga gggcgcgtgg acggaggtga cgcttccgcg ggtgcgcacg ccggcgctcg
     1081 ccgagccacg ctttctgttc gacggcgacg agctcctgga ctggcgtgag gcgccgggcg
     1141 agccgctcag ggcggcgcag ccgatggcga tcgagccggg ccaggtggcg gtgcacgacg
     1201 gcgagctgtg ggtcctcgcc tcggtgccgg agatcggcca cggcgatcag tcacggcagg
     1261 tcgtgctgcg ttcgcgtccg gtgcagcaga tcctcgagct gacggacagc gtgtcgctcg
     1321 gggccgagat gagccccggg tcactggcga cgagcctcga cccgaagcgg ggctgtaaca
     1381 ccgggtcgac gtgggtggtc gtcgcgacga tcgcgtccgg gactgaccga gacgccggtg
     1441 ccgcggtggc ggagtcgttc ctggcgagcc tgccggacga gctgcgcgag gggctggttg
     1501 cgctgcgcga cttccgtgtg cgcggccgca gggtggtggc cgcgacgatg ggcttccacg
     1561 acatggaggc gaccatggcc ctggccgagg cgaccaggcg catccggccc gacgagaagc
     1621 acccgttcga gtgcggcaac ccgcgaccga tccgcagctt ccacgagcac gactgagatc
     1681 aggccggcgg cgcgggcgga ggcgccggag gggcgcgacg cagcacggcc gcgagcgcga
     1741 tgccgccgac gagctcgccg taccacagtg tacacgcacg gatcagcagg gtcgtggcga
     1801 gcgcggccgg ctgtgtgacg ccagtggcga gccgcgcgac gagcacggcg agagatccct
     1861 cggtggcccc gagcccgccg ggcaggaagc tcagcgcgcc gatcagggtg gtcgtcgacc
     1921 acaggaaggt gcaaagcgcg aggctggcct cggcgccgac caggccgcgc acgatcagcc
     1981 agtagccgat acactcgagg ccccagccga ccacgctgat cagcgagagg accagcagcg
     2041 gtcgcaggcg cagcagcgcg gcgctgctgt gcacgagggc ctcggcgcgg ccgccgaggc
     2101 ggcccaggcc gggcaggcgg gacagcgagt gcaggaggcc gaggcacagc cgcggcgagc
     2161 cgagcacgac gacgccggcg accaccagcg cgagggtgat cagcacgacc ggcaggtact
     2221 cgcggaactg actgacgccg acgaggctga gcacgacgag cgcgacgagg tcggtcaggc
     2281 gatcggcgac gacgaccggc gcggtgcgag agaagggcac gccatcggtg aggcgcagga
     2341 ggtagctgcg cagcacctcg ccgaccttgc cgggcgtgac cgacatcgac agacccgcga
     2401 ggtagatcag cagcgagcgg ccgcgcgtca ggccgggggc gtcctcgggg ccgtcgccgc
     2461 ggacgccgag ccagccgaga cacagctccc acttcaggaa gcgcaggaga tagttgacgc
     2521 tcgacagccc gagcgcgagc aacacggccg accacgcgta gccggcgagc gccgcgcgga
     2581 cgcggccgat gtcgacgtag agcacggcgc cgacgtagag cagcacgccg aggacgaccc
     2641 caaacagcgc gcgctggacg aagcggctca tgcgggcggc gagcataccc acctcgccgc
     2701 ggggcgacaa acggcgatcc gtcgcctggc gtgtggacgc gcttcatttc cgggcggcag
     2761 accgcttgtc acggccgcgc gcgcgcggca ccctgcccgc catgtcgacg agccagggag
     2821 cggtattttg ggtgggtcgg gtcgccacac cggcgggtcc acgctgggtg cgggtgttca
     2881 ccgagggcgc gaagccggcg ccggaggacc cgatcgagat cgtcgtcgac cccttcgccg
     2941 acgagcacgg agatccgtac gcccgcgtgc gtgcgagcgt gcgtggcgag ccggtcggca
     3001 ccgtcggcac cgcgaagctg ctgccgccgg tgcgcccgag caaggtcgtg
//
LOCUS       DASWNJ010001728         1702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_101950, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001728 DASWNJ010000000
VERSION     DASWNJ010001728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101950"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VGB85_28735"
     CDS             complement(<1..674)
                     /locus_tag="VGB85_28735"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEY0138120.1"
                     /translation="MSGLSRFLPCLRWSRGYTRGDLTGDLLAGATTAAFLIPQAMAYA
                     SLAGLPPITGLYAALAPPIVYALLGTSRRLAVGPVAIDSLLTAAALAPLAAGDPLAYA
                     AAASALALLVGAIQATTGMLRLGFIVNFLSLPVLRGFTAAAAILIMASQLGPLLGLRL
                     PASAGLVPTLRALVEQIAATHVATAGLGLAVVGGLLLMGSFRGRALVVLTVGVVLGHW
                     LALDTHG"
     gene            complement(764..1189)
                     /locus_tag="VGB85_28740"
     CDS             complement(764..1189)
                     /locus_tag="VGB85_28740"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY0138121.1"
                     /translation="MCRELVTRRVFHAVAHFWRVKPIAAGSRPRTTCGPASDERREEA
                     DTREVPSRRVHARRAPLQDVQYLERGYGRPPHHRSQRDAGGGYVPENGISLCPQCHEK
                     AEAFHRTGAALPGFSPDELYARVGSSRALALRASARRAR"
     gene            1247..>1702
                     /locus_tag="VGB85_28745"
     CDS             1247..>1702
                     /locus_tag="VGB85_28745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138122.1"
                     /translation="MALESISHEVDGASLTWDLSRGTLNFLGLPSALLWLDPSLRRLI
                     EPLVHEVGAPLYRLLTAHHASLGTDADHATIMAAGDFETGFRGWASAVAVAGWGRFTL
                     LAYDPKTPRARVRVDSPWELELQRSLPPERRWGCPFAQGKLIGLFCHALA"
BASE COUNT          244 a          629 c          600 g          229 t
ORIGIN      
        1 ccgtgggtgt cgagcgccag ccagtgcccg agcaccacgc cgaccgtgag caccaccagc
       61 gcccggccgc gaaagctgcc catcagcagc agcccgccga ccacggcgag cccgagcccc
      121 gcggtcgcca cgtgcgtcgc cgcgatctgc tcgaccagcg cccgcagcgt cggcaccagc
      181 ccggcgctgg ccggcagccg cagcccgagc agcggtccga gctggctcgc catgatcagg
      241 atcgccgccg cggccgtgaa gccgcgcagc accggcagcg acaggaagtt gacgatgaag
      301 ccgagccgca gcatcccggt cgtcgcctgg atcgcgccga ccagcagcgc gagcgcggac
      361 gcggcggccg cgtaggcgag cgggtcaccg gcggcgaggg gcgcgagcgc ggcggcggtc
      421 agcagcgagt cgatcgccac cggtccgacc gcgagccggc gcgaggtgcc gagcagcgcg
      481 tacacgatcg ggggcgcgag cgcggcgtag agcccggtga tcggcggcag cccggcgagc
      541 gacgcgtagg ccatcgcctg cgggatcagg aacgccgccg tggtcgcgcc cgccagcagg
      601 tcgcccgtca ggtccccgcg cgtgtagccg cgagaccagc gcaggcacgg caggaagcgc
      661 gagaggccgc tcatggccga cacaatgcac ccgatgcgcc gcccgtgggg gacgccgatc
      721 acgatcgccg ttccggccgc ggcgacaggc cttgtccggg ggctcaacgc gcccggcggg
      781 cgctcgctcg cagcgccagg gcccgcgacg agccgacgcg cgcgtacagc tcatcggggg
      841 agaaccccgg cagggcggcc ccggtgcgat ggaacgcctc ggccttctcg tgacattgtg
      901 ggcacagcga gatgccgttc tcgggcacat accctccccc ggcatctcgt tgcgatcggt
      961 gatgtggtgg gcgtccatat cctcgctcga ggtattgcac gtcttgcagc ggtgcccgtc
     1021 tcgcgtgaac acggcgtgac ggaacctctc gcgtatctgc ttcttctcgg cgctcatcgc
     1081 ttgcaggccc acatgtcgta cgcggccgcg atccggcagc gattggtttc acccgccaaa
     1141 aatgcgcgac agcgtggaac acgcggcgcg tgacgagctc gcgacacata ttaatcccgg
     1201 cgttccaccc tgggcaaccg cgggctcgct gctaccctgt gccgccatgg cattggagtc
     1261 catcagccac gaggtcgacg gggcgagttt gacgtgggac ctgtcccgcg ggaccctgaa
     1321 cttcctcggc ctgccctcgg cattgctctg gctcgacccc tcgctgcgcc gcctgatcga
     1381 gcctctggtg cacgaggtcg gcgcgccgct ttatcgcctg ctgacggccc atcacgcgag
     1441 cctcggcacc gacgccgacc acgcgacgat catggcggcc ggtgacttcg agaccggctt
     1501 tcgcggctgg gcctccgcgg tcgcggtcgc tggctggggc cgcttcaccc tgctcgcgta
     1561 cgacccgaag accccgcgcg cgcgcgtgcg tgtcgacagc ccgtgggagc tcgagctgca
     1621 gcgcagcctg ccgcccgagc gccgctgggg ctgtccgttc gcgcagggca agctgatcgg
     1681 gctgttttgc cacgcgctcg cg
//
LOCUS       DASWNJ010001729         5067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_135694, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001729 DASWNJ010000000
VERSION     DASWNJ010001729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135694"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VGB85_28750"
     CDS             <1..392
                     /locus_tag="VGB85_28750"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0138123.1"
                     /translation="KLGWETPRILAAMVDAPDFYFDSMSQIYLDRWSQGRVALVGDAA
                     CCASPMSGQGSSIALVSAYVLAGELAAAGGEHRQAFAAYEAVLRDYVARNQQLALLAA
                     ERRGPPTAEETREASALELRDYSTALS"
     gene            630..1982
                     /locus_tag="VGB85_28755"
     CDS             630..1982
                     /locus_tag="VGB85_28755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138124.1"
                     /translation="MAFVAPIACYDGSPEFDAPDVLPREGVNGEECAMSDYNCKLRPG
                     SPRVTTNDPDDNDYWGLMTGVQILDGNGDPMGVQSRDRAIFNYGQTRVFNGETHAFAM
                     STSNSSTGWLPVSSILGRASFEAKVGHVSAKGAKLAKMACYQIRSSHDAALGSKVVVA
                     YFPKDERLPLASDYLPLPRANGRRSANLAFNVPGFSLAGAAVDHFPAGTKFQRLEVTT
                     WEENGRLPSIEIDLYHVVDQNGQPTGATRLDANTRLEPTGAKMKFIYGYVIARTGTKR
                     NGWMALDALEVSDSCDAALTPPPDEPPPPPPPPPPEPPPEPPPLPPETDPPPDEPAPP
                     PEPTPEPPPPPPPPAPNQCYVRCCDESLQGPVDTPDPAACHDASSVMCSNNEHVKRSE
                     WNGQQVWERPNSCWAKCFNREAYHLVDVQQDCAAHAGAYCAESDRGGLEDAAWSQCQP
                     "
     gene            complement(2073..2951)
                     /locus_tag="VGB85_28760"
     CDS             complement(2073..2951)
                     /locus_tag="VGB85_28760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0138125.1"
                     /translation="MTDQETEELARLRGRVAELEASQAGCHAELEALRRTEAELRAMF
                     RGMTDLVLVMDRDGRYLRIAETAPGLLYRPPGDLVGKRMHEVMPDAAAEFFLQKIHEA
                     LASQALVSMDYSLPIEDRELWFSANISPMSDDTVLLVCRDVTERKHHEQVLQNSLRQQ
                     EQLRAQEAELMQLSTPLIPIGADIVVMPLVGQLNAQRAQQVMETLLQGIERTRVRVAI
                     LDITGVARVDTAVADALVNAARAVKLLGARAVLTGIRPEVADVLVNLGVDLSGIRTCG
                     TLQDAIREAQSQTRRR"
     gene            3302..3424
                     /locus_tag="VGB85_28765"
     CDS             3302..3424
                     /locus_tag="VGB85_28765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138126.1"
                     /translation="MGDEPNARLDGFKAHKEANDACAELPDDVADRLKDRLHMK"
     gene            3473..3805
                     /locus_tag="VGB85_28770"
     CDS             3473..3805
                     /locus_tag="VGB85_28770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138127.1"
                     /translation="MAPRAPTGRPAVLAWLRSPAAGVTPPWLPLGNPSMLAAVLHEDG
                     VWRVRKTAVDTEAFHAFIKALQAEGRPLFPEHVDRFHRPTGEVMLEAASLDEFIRAVE
                     AYDWPGNW"
     gene            complement(4085..>5067)
                     /locus_tag="VGB85_28775"
     CDS             complement(4085..>5067)
                     /locus_tag="VGB85_28775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138128.1"
                     /translation="QDVHRDQIQFLASAVLSPGESATLPDMLFEPVESFIYCSLEALR
                     RDGALSRFVSLLAEFRPPLVDRLRDVEGELRPHQGMSSPLWRTWDSIPAKDPGTYLNK
                     YLKHATKAFISPRWDDFRKRHEGAMLLWQRRMTDPIAPLPPLPAFPEQALALLLTAMY
                     GSVELRLLAARLGPNVSHRTPDFRTSRLTFAGMLVSAVADNRRQGAFFALLGQQRPRW
                     AQEIGGIALNFGFTPDEYEQVAIPEPSFEWRLGELIFEATAGLDPLWDSLLIPTQEVL
                     GPFVAPRLRAQYAAEWLVRQGLIERPLFSRLSAIAPHLGPQIAELQVEVA"
BASE COUNT          863 a         1740 c         1692 g          772 t
ORIGIN      
        1 gcaagctcgg ctgggagacg ccgcggatcc tcgcggcgat ggtcgacgcc cccgacttct
       61 acttcgactc gatgagccag atctacctcg accgctggtc gcagggccgt gtcgcgctcg
      121 tcggtgacgc ggcctgctgc gcctcgccga tgtcggggca gggctcgagc atcgccctcg
      181 tgtccgccta tgtgctcgcc ggagagctcg cggccgccgg cggcgagcat cgccaggcgt
      241 tcgcggccta cgaggccgtc ctgcgtgact acgtcgctcg caatcaacag ctcgcgctgc
      301 tggccgccga gcgtcggggc ccgccgaccg ccgaggagac gcgcgaggcc agcgcgctcg
      361 agctccgcga ctactcgacc gccctttcgt gaatcggtgg aggcgtcctc ccacatacat
      421 gcatgatgag catgatgacg ccggcgcgat agcgatcccg catgaccggc gcgcgcgacg
      481 ctgctcgaat gcagcgtcgc ggccagacat cgtggtgatg ccagtcaccg tggcacatcg
      541 tgatgccatg tatcttggcg ggcccgaaaa ggatcgacat gactcgaacg aaccggcgat
      601 cttcgactgt ctcgtgctgc ggcgcgctca tggcgtttgt cgcaccgatc gcgtgctacg
      661 acggatctcc ggagttcgac gcgccggacg tgctcccgcg ggagggggtc aacggcgaag
      721 agtgtgcgat gagcgattac aactgcaagc tccgccccgg gagcccgcgc gtcaccacca
      781 atgaccccga tgacaacgac tactgggggt tgatgaccgg cgtgcagatc ctcgacggca
      841 acggcgaccc gatgggcgtg cagagccgcg accgcgccat cttcaactat ggccagaccc
      901 gcgtcttcaa cggcgagacc cacgcgttcg cgatgtcgac ctcgaacagc agcaccggct
      961 ggctgccggt ctcatcgatc ctcgggcggg cctcgttcga ggccaaggtc gggcacgtct
     1021 ccgcgaaggg ggccaagctc gccaagatgg cctgctacca gatccgcagc tcgcacgacg
     1081 cggcgctcgg cagcaaggtg gtggtcgcgt actttcccaa ggacgagcgg ctgccgctcg
     1141 ccagcgatta tctgccgctg ccgcgcgcca acggccggcg ctcggccaac ctggcgttca
     1201 acgtgccggg cttctcgctc gccggcgcgg cggtcgatca cttcccggcg ggcaccaagt
     1261 tccagcggct ggaggtgacg acctgggagg agaacgggcg cctgccctcg atcgagatcg
     1321 acctgtatca cgtcgtcgat cagaacggcc agccgacggg ggcgaccagg ctcgacgcga
     1381 atactcgcct cgagcccacc ggcgccaaga tgaagttcat ctacggctac gtgatcgcca
     1441 ggaccggcac gaagcgcaat ggttggatgg ccctcgacgc gctcgaggtc agcgacagct
     1501 gcgacgccgc gctgacaccc ccgcccgacg agccgccgcc gccacccccg ccgccgccgc
     1561 ccgagccgcc acccgagccg ccgccgctcc cgcccgagac cgatccgccg cccgacgagc
     1621 cggcgccgcc gcccgagccg accccggagc cgccgccccc gcccccgccg ccggccccga
     1681 accagtgcta cgtgcgctgc tgcgacgaga gcctgcaagg gcccgtcgac acgccggacc
     1741 ccgcggcctg ccacgacgcc tcgtcggtga tgtgcagcaa caacgagcac gtcaagcgct
     1801 cggagtggaa cggccagcag gtgtgggagc ggcccaacag ctgctgggcg aagtgcttca
     1861 accgcgaggc ctaccacctc gtcgacgtcc agcaggactg cgcggcgcat gccggggcct
     1921 actgcgccga gtccgaccgc ggcggcctcg aggacgccgc gtggagtcag tgccagccct
     1981 gaaggacatg tcctccagga caggccgctg accgcgggat caccatgata cgagcgccgg
     2041 cgcgcgccgg cgctcgtggt gtggtgagcg cctcagcgcc tgcgcgtctg tgactgcgcc
     2101 tcgcggatcg cgtcctgcag cgtcccgcag gtgcgaatgc cggagaggtc gacgccgagg
     2161 ttgacgagca cgtcggcgac ctccgggcgg atccccgtca ggactgcgcg cgcgccgagc
     2221 agcttgaccg cccgcgccgc gttgaccagc gcgtcggcga cggcagtgtc gaccctggcg
     2281 accccggtga tgtcgaggat cgccacgcgg acccgcgtgc gctcgatccc ctgcagcagc
     2341 gtctccatca cctgttgcgc gcgctgggcg ttgagctggc cgaccagcgg catcacgacg
     2401 atgtcggcgc cgatcgggat cagcggcgtc gacagctgca tcagctcggc ctcctgcgcg
     2461 cgcagctgct cctgctggcg caggctgttc tgcaggacct gctcgtggtg cttgcgctcg
     2521 gtgacgtcgc ggcagaccag cagcaccgtg tcgtccgaca tcggcgagat gttggcggag
     2581 aaccacagct cgcggtcctc gatcggcagg ctgtagtcca tgctgaccag cgcctgtgac
     2641 gcgagcgcct cgtggatctt ttgcaggaag aactcggcgg ccgcgtccgg catgacctcg
     2701 tgcatgcgct tgccgacgag gtcgccgggc gggcggtaca gcagcccggg cgccgtctcg
     2761 gcgatccgca ggtagcggcc gtcgcggtcc atcacgagca cgaggtcggt catcccgcgg
     2821 aacatcgccc gcagctccgc ctccgtgcgg cgcagcgcct cgagctccgc gtggcacccg
     2881 gcctgcgagg cctcgagctc cgccacgcgt ccgcgcagcc gcgcgagctc ctccgtctcc
     2941 tgatccgtca ctggcgacga tgctaacagg atccacgtgc gcccgtcacg gtcatgcagg
     3001 tctgcacagg cagcggtcgc gacgtcgaac tgcgctggat atggcacgaa cgcaagcacc
     3061 gatctcgacg cgacaaagat cgttctccag ggcacgtgcc gcggcgtcat cgaaggcgcc
     3121 ggatcgctcg gcgaccacgg gccgtcgcgt gggctggaga gcgctccgcc gcgccgcccc
     3181 gacgcgttgt tgcccgcgcc ggctctgcga tggtcggcgt tcggagacct ggcgagtgcg
     3241 cggaggatag ggccccggcg gcgccgcgat cgacgaggag accgcctgcc acgacgccgt
     3301 catgggcgac gagcccaacg cccggctcga cggcttcaag gcccacaagg aggcgaacga
     3361 cgcgtgcgcc gagctcccgg acgacgtcgc ggacaggctc aaggaccgcc tgcatatgaa
     3421 atgaagccga cgcggtgtcg tcgtccgcga gcaggggtgt atattcgcgg gcatggctcc
     3481 acgcgctccc acaggccgcc ctgcggtgct cgcctggctg cgatcgcccg ccgccggcgt
     3541 cacgccgccg tggctaccgc tcggtaatcc ctccatgctg gcggccgtgc tgcacgagga
     3601 cggcgtgtgg cgcgtgcgca agacggcggt cgacaccgaa gcgttccacg ccttcatcaa
     3661 ggccctgcag gccgaggggc gcccgctgtt ccccgagcac gtcgaccgct ttcaccgccc
     3721 gaccggcgag gtgatgctcg aggcggcgag cctcgatgaa tttattcgcg cggtcgaggc
     3781 atacgactgg ccgggcaatt ggtagcactt taccaagtgc gccgatgcag cgttgcgcgt
     3841 cgagtactgg attatctata atacctcacg ggtgaggccg catgacccgc ggtcgaggca
     3901 cgtcgcaacg gcgagcaccg tgcacaccgc atcaagtcaa gagagcagag cccgtaggcc
     3961 ggcccgaccg cgggtcgagg gctggaagat ccgtttacca agccggcgcg cgcacaagtg
     4021 cgtctacatc gctacgaccc tcgcgggaca ccaccgtgtg tcaagccgct tgttcggtgt
     4081 atcgttacgc gacctcgacc tgtagctctg caatttgcgg acccaagtgg ggagcgatcg
     4141 cggacaatcg agagaaaaga ggtctttcaa tcaggccttg acggacaagc cactccgcgg
     4201 cgtactgcgc ccgaagccgt ggggcaacga aagggccgag gacctcctga gtaggaatga
     4261 gtagagagtc ccagagcgga tccaggccag ccgtagcctc gaagatcaat tcgccgaggc
     4321 gccattcaaa cgaaggctcg ggtatggcta cctgctcgta ttcgtctggc gtaaaaccga
     4381 agttcagggc tatcccgcca atctcctggg cccaacgcgg acgttgctgg ccaaggagcg
     4441 cgaaaaaagc gccctgacga cggttgtcgg ccacggcgct gaccagcatg cctgcgaagg
     4501 tcaatctgga tgttctgaaa tcaggtgttc ggtggctgac gttcggcccc aatctggcag
     4561 cgagcaggcg aagctccacg ctaccgtaca tggcagtgag cagcaaggcc agggcctgct
     4621 ctgggaacgc gggcagggga gggagtggtg cgatcgggtc cgtcatgcgg cgctgccaga
     4681 gaagcatcgc gccttcatgg cgttttcgga agtcgtccca ccttggcgag atgaaggcct
     4741 tggtcgcatg ctttagatac ttattgagat atgtacccgg gtccttcgcc gggatcgaat
     4801 cccaagttcg ccacaggggc gagctcatcc cttgatgtgg cctcagttcg ccctcgacgt
     4861 ctcgcaagcg atcgaccaga ggaggccgga attccgccaa tagcgatacg aagcgcgata
     4921 gggcgccatc acgtcgcaat gcctctagcg aacagtagat gaaagactcg acaggttcga
     4981 agagcatgtc gggcaacgtc gccgattcgc cggggctcag aacagccgat gccaggaatt
     5041 ggatctgatc tcgatgtacg tcttgca
//
LOCUS       DASWNJ010001730         7235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_30326, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001730 DASWNJ010000000
VERSION     DASWNJ010001730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7235
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30326"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VGB85_28780"
     CDS             <1..551
                     /locus_tag="VGB85_28780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138129.1"
                     /translation="TKALEPLLAPLRKPAVAALLGEADLELDADDDLLEHLGKAVPKD
                     SLIAFGQHGSGSLLAVWRTDPRMPLGRCPIIWLDSEGDPIEPLAVDFAAFLQLLPYGM
                     GQLYDLLVKARRMRDGDDGDDEAAAFEVDTDDLREGLAMISSELGAWREAGLPPASDP
                     LALVEQAVALPFAAWWDDLPDA"
     gene            612..2369
                     /locus_tag="VGB85_28785"
     CDS             612..2369
                     /locus_tag="VGB85_28785"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEY0138130.1"
                     /translation="MFRIRRIHDDLLPVNAAAIAQVQAQLRVQFPDLRASQIDALPDQ
                     LRDPMAYQFRTVVFVAERGHNVRGFAVIMHAPDLHFCFLEFISAAPGEGGRGVGGALY
                     ERLQDECREIGAVGLFFECLPDDPAKVTDPAKLAENRARMRFYERLGARPITNTAYDT
                     PLTPDDVDIPYLMYDDLGRGKPLRRDAARNIVRAILERKYDWLCPPEYVDRVVTSIVD
                     DPVRLRPPRYTRTATERKASPPLPADLRIPLVFNDQHQIHHVRERGYVEAPVRIPAIL
                     RELERLETFTVIEPKTFPKSHILAVHDPEFVGYLQRVCADLPEGRSVYPYVFPIRNAT
                     RPPADLATRAGYYCIDTFTPLHRNAWLAARRAVDCAMTAATHVLSGSRIAYALVRPPG
                     HHAERRVFGGFCYLNSAAVAANFLSAQGKVAMLDVDYHHGNGQQDIFYERADVLTVSL
                     HGHPRFAYPYFSGFEDERGAGPGEGFNRNFALPEQLDGPGYAPHLERAIAEVRAFRPR
                     FLVVCLGLDPGKGDPTGTWTLGSADFERNGKLIGALGLPTLVVQEGGYLTRSLGAHAR
                     SFFRGLWEGIHARRLRPGE"
     gene            complement(2597..2749)
                     /locus_tag="VGB85_28790"
     CDS             complement(2597..2749)
                     /locus_tag="VGB85_28790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138131.1"
                     /translation="MLLPVPGSLAVVDPSVLSTGDVAANDVPFAGAGVQALHIRLSGT
                     VRTTLA"
     gene            2817..3677
                     /locus_tag="VGB85_28795"
     CDS             2817..3677
                     /locus_tag="VGB85_28795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138132.1"
                     /translation="MLWDLGTPQDLGDPAPPGCKGKVDFLFAMSRGPNMAQIQEQFIA
                     SFEPFITSIEGLGDEFEDFDFHIMVVDGDDHWGSVYCDDKCEQDGVCDLTVSYPCDYE
                     PTACDQALGAGTIYNAGRDAPNKHCGLAGGKRYLDKSNFGLQETFACIAQVGSAGEDD
                     LGNALAQAVTTQRDTCNSGFLRDDALLVVVFIMTGDVLPGVDGIPQYWANALIDAKGG
                     DLDAIVMLGMAGAGPFEKCVDYYDNEMCQMLALFPHRVWADPNIADYGPAFQETLALI
                     DVACEEFAPG"
     gene            complement(3697..5373)
                     /locus_tag="VGB85_28800"
     CDS             complement(3697..5373)
                     /locus_tag="VGB85_28800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138133.1"
                     /translation="MRPATVSFLLVVLAAGCGDAPALASLTSAATETQHSSSTGAGDR
                     TTGTDAEPPDSETGTSGSTDTGDTTIEGGATGELGSGSGEPAPPPTLREATLTPDVIA
                     FEGPVVVHALADDAEGVRMRVDGGEPVELTLVAPGEFAGEIGVFTALSNGTHDVVFTA
                     WQGEEESAPLLVQYEAKLRNAGGEAYWDASGVLGEGFGRGVAVDQVSGQIYELFDVDE
                     GGEQRCYVRQRGAQGEYFDADLVAVLPGKSCTATAITATADGTLYVLAETELNGQKVW
                     WLGRMVSWKAPLLTLDLGEPGEEAHALAQHPDSGDVAVCGTMPTNKKDEVDGMVVIFR
                     EGLPGYRPAPFDYKPTPDDFPNQFGETLRDCAYAGGTLVLTGELFGVHPDYKDEGETT
                     RLVLVEYDVQADAAHWTVAGIGPGSAVQSGARSLVVTAEGTYLTLGHTCELECEPQLH
                     LREFLPGGVPSGWNVLPAVDASATDLAWSPAGYAVLAAAKVKGPWWTEFWLQAWVPGD
                     EVPAWSYGHPDQPDLHIVFAIAIAEYGRIYAVGVAGQGDLHVPALAIVDP"
     gene            complement(6045..7016)
                     /locus_tag="VGB85_28805"
     CDS             complement(6045..7016)
                     /locus_tag="VGB85_28805"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HEY0138134.1"
                     /translation="MSLPVVDISPLVHGDAVARARVASEIAAACRDSGFFYAVGHGVA
                     PELFSRVMAASREFFALPLAQRQEIAMARGGRAWRGYFALGEELTSGRPDRKEGLYFG
                     TELAQDHPRVRAGVPLHGANLFPAQVPALREAVLEVMCALTGVGHVLMEGVALALGLD
                     AEYFKLHYTADPTVLFRVFHYPPGDDSDWGVGEHTDYGLLTLLVQDEVGGLEVRTPRG
                     WICAPPIPGALVCNIGDMLDRLSGGEFRSTPHRVRNTSGRSRLSLPLFFDPDFDAQVR
                     PLPAREAIDDHASRWDGRSVHLFDGTYGEYLKSKISRVFPALFGELL"
BASE COUNT         1115 a         2741 c         2314 g         1065 t
ORIGIN      
        1 ccaccaaggc cctcgaaccc ctcctcgccc cgctgcgcaa gcccgccgtc gccgccctgc
       61 tcggcgaggc cgacctcgag ctcgacgccg acgacgacct cctggagcac ctcggcaagg
      121 ctgtcccgaa ggacagcctg atcgcgttcg gccagcacgg cagcggctcg ctgctcgcgg
      181 tctggcgcac cgacccacgc atgccgctcg ggcgctgccc gatcatctgg ctcgacagcg
      241 agggcgaccc gatcgagccg ctcgccgtcg acttcgccgc gttcctgcag ctcctcccgt
      301 acggcatggg ccagctctac gacctcctcg tcaaggcccg gcgcatgcgc gacggcgacg
      361 acggcgacga cgaggccgcg gccttcgagg tcgacaccga cgacctgcgc gagggcctcg
      421 ccatgatctc cagcgagctc ggcgcgtggc gcgaggccgg gctgccaccc gcgagcgacc
      481 cgctcgcgct cgtcgagcag gccgtcgcgc tgccgttcgc cgcgtggtgg gacgacctgc
      541 cggacgcctg accgcggccg ctgctggttg catatggttg catcatgacc ccgcgggtgt
      601 aatcctcgcg ggtgtttcgc atccgccgga tccacgacga tctgctgccg gtgaacgccg
      661 cggcgatcgc ccaggtgcag gcccagctgc gggtccagtt cccggacctg cgcgccagcc
      721 agatcgacgc gctgcccgat cagctgcgcg acccgatggc ctaccagttt cgcacggtcg
      781 tcttcgtggc cgagcgcggg cacaacgtcc gcggcttcgc cgtgatcatg cacgcgccgg
      841 acctgcactt ttgcttcctc gagttcatct ccgccgcgcc cggcgagggc ggccggggcg
      901 tcggcggggc cctgtacgag cgcttgcagg acgagtgccg ggagatcggc gccgtcggcc
      961 tgttcttcga gtgcctgccc gacgaccccg ccaaggtcac cgacccggcc aagctcgccg
     1021 agaaccgcgc gcgcatgcgc ttctacgagc gcctcggcgc gcggccgatc accaacaccg
     1081 cgtatgacac cccgctcacg ccggacgacg tcgacatccc ctacctgatg tacgacgacc
     1141 tcggccgcgg caagcccctg cgccgcgacg ccgcccgcaa catcgtgcga gcgatcctcg
     1201 agcgcaagta cgactggctg tgcccgcccg agtacgtcga tcgcgtcgtc acttcgatcg
     1261 tcgacgaccc cgtgcgcctg cgtccgccgc gttacacccg caccgcgacc gagcgcaagg
     1321 cctccccccc gctgcccgcc gacctccgga tcccgctcgt cttcaacgac cagcaccaga
     1381 tccaccacgt ccgcgagcgc ggctacgtcg aggcgccggt gcggatcccg gcgatcctcc
     1441 gcgagctcga gcgcctcgag accttcaccg tcatcgagcc caagaccttc ccgaagagtc
     1501 acatcctcgc ggtgcacgac cccgagttcg tcggctacct gcaacgcgtc tgcgccgacc
     1561 tccccgaggg ccgcagcgtc tatccctacg tgttcccgat ccgcaacgcg acgcgtccgc
     1621 ccgccgacct cgccacccgc gccgggtact attgcatcga caccttcacg ccgctgcacc
     1681 gcaacgcgtg gctcgcggct cgccgcgcgg tcgactgcgc catgacggcc gcgacccacg
     1741 tgctgtccgg ctcccgcatc gcctacgcgc tggtgcgtcc gcccgggcat cacgccgagc
     1801 gcagggtgtt cggcggcttt tgttacctca acagcgccgc ggtcgccgcc aacttcctgt
     1861 ccgcccaggg caaggtggcg atgctcgacg tcgactatca ccacggcaac gggcaacagg
     1921 acatttttta cgagcgcgcc gacgtgctga ccgtctcgtt gcacggccac ccgcgcttcg
     1981 cctatcccta cttctcgggc ttcgaggacg agcgcggcgc cggcccgggc gagggcttca
     2041 accgcaactt cgcgctacca gagcagctcg acggccccgg ctacgcgccg cacctcgagc
     2101 gcgcgatcgc ggaggtccgc gcgtttcgtc cgcgcttcct cgtcgtctgc ctcggcctcg
     2161 accccggcaa gggcgacccg accggcacct ggaccctcgg ctccgccgac ttcgaacgca
     2221 acggaaaact gatcggcgcg ctcggcctgc cgaccctcgt cgtgcaggag ggcggctacc
     2281 tgacccgcag cctcggcgcc cacgcccgca gcttctttcg cggcctgtgg gagggcatcc
     2341 acgcccgccg cctgcgcccc ggcgagtgac agttggcggc ttcgcaatac cacacacgcc
     2401 gccgtggcct cgctatcctc gagagcctcg gtatccgcgc gaagcgggcc gcgctgcacc
     2461 tgtaacgatg tcgcgagacg atgaatcgac gggcccgccc ggctgttcgg acccctccga
     2521 taactgggta tccaacgact aacctcggac gcgcaaactc gcgcgactca tgctaccgtc
     2581 tgcgcatgcg cggtcttcac gccagcgtgg tcctgacggt gccactgagc cttatgtgga
     2641 gcgcctgcac ccccgcaccg gcgaacggaa catcgttcgc cgcgacgtcg ccggtgctca
     2701 gcaccgaggg gtcgacgacc gccagcgatc cggggacagg aagcagcacc tcggacggtt
     2761 cagggcccgc ggacagctcg agcgggtccg ctggctcgtc cgggacgagt gccgggatgc
     2821 tgtgggacct ggggacgccg caagacctcg gcgaccccgc gccgcctggc tgcaagggca
     2881 aggtcgactt cctgttcgcc atgtctcgcg ggccgaacat ggcgcagatt caggagcagt
     2941 tcatcgccag cttcgagccg ttcatcacgt cgatcgaggg cctcggcgac gagttcgagg
     3001 acttcgactt tcatatcatg gtggtggacg gagacgatca ctggggctcg gtgtactgcg
     3061 acgacaaatg cgaacaggat ggagtctgcg acctgacggt cagctaccca tgtgactacg
     3121 agcccacggc gtgtgaccag gcgctcggcg ccggcacgat ctacaacgcc ggccgggacg
     3181 ctcccaacaa gcactgtggt ctggcgggcg gcaagcgcta cctcgacaag agcaacttcg
     3241 gcctccagga gaccttcgcc tgcatcgcgc aggtcggctc cgccggcgaa gacgacctcg
     3301 gcaacgccct cgcccaggcc gtgaccaccc agcgcgacac ctgcaacagc ggcttcctcc
     3361 gcgacgacgc gctgctggtc gtcgtgttca tcatgacggg cgacgtgctc ccgggtgtcg
     3421 atggcatccc gcagtactgg gccaacgcgc taatcgacgc caagggtggc gacctcgacg
     3481 ccatcgtcat gctcggcatg gccggggcgg gtccattcga gaagtgcgtg gactactacg
     3541 acaatgaaat gtgccagatg ctcgcgttat tcccgcaccg ggtatgggcc gacccgaaca
     3601 tcgcggatta cggtccggcg tttcaggaga ccctcgcgct catcgacgtc gcctgcgaag
     3661 agttcgcgcc aggctgagcg cgccgccgac gccggctcac ggatcgacga tggcgagcgc
     3721 gggcacgtgg aggtccccct ggccggcgac gccgaccgcg tagatgcggc cgtactcggc
     3781 gatcgcgatt gcaaatacaa tatgcaggtc gggctggtcc ggatggccgt agctccacgc
     3841 cgggacctcg tcgccgggca cccaggcctg cagccagaac tcggtccacc acggaccctt
     3901 gaccttcgcc gcggcgagga ccgcgtagcc ggccgggctc cacgccaggt cggtcgcgga
     3961 cgcatcgacc gcgggcaaca cgttccaccc ggagggcaca ccgccgggca ggaactcacg
     4021 cagatgcagc tgcggctcac actcgagctc acacgtatga cccagggtca ggtacgtccc
     4081 ctcggccgtg acgacgagcg agcgggcgcc gctctgcacc gcggacccgg gtccgatccc
     4141 ggcgacggtc cagtgtgccg cgtcggcctg gacgtcgtac tcgacgagca cgaggcgggt
     4201 tgtctctcct tcgtctttgt agtctgggtg gactccaaac agctcacccg tgagcaccag
     4261 cgtgcccccc gcgtacgcgc agtcgcgcag cgtctcgccg aattggttcg ggaagtcatc
     4321 aggcgtcggt ttgtagtcga acggcgccgg cctgtacccc ggcaggccct cgcggaagat
     4381 caccaccatc ccgtcgacct cgtccttctt gttcgtcggc atcgtgccgc agaccgccac
     4441 atcccccgag tcggggtgct gcgccagcgc gtgggcctcc tcgccgggct cgcccaggtc
     4501 gagcgtcagc aacggtgcct tccagctgac catgcggccg agccaccaga ccttttggcc
     4561 gttgagctcc gtctcggcga gcacgtacag cgttccatcc gcggtggccg tgatcgcggt
     4621 cgccgtgcac gactttcccg gcagcacggc gacgaggtcc gcgtcgaagt actcgccctg
     4681 cgcgccgcgc tggcgcacgt agcagcgctg ctcgccgccc tcgtcgacat cgaacagctc
     4741 gtagatctgg ccgctgacct gatcgaccgc gacgccccgt ccaaagccct cgccgagcac
     4801 acccgaggcg tcccagtacg cctcgccgcc cgcgttcctc agcttcgcct cgtactggac
     4861 gagcagcggc gcgctctcct cttcaccctg ccaggccgtg aacacgacgt catgggtgcc
     4921 gttggacagc gccgtgaaca caccgatctc acccgcgaac tcgccgggcg cgacgagggt
     4981 gagctcgacc ggctcgccgc catcgacgcg catgcgcacc ccctcggcgt cgtcagcgag
     5041 cgcgtggacg acgacggggc cctcgaacgc gatcacgtcc ggggtcaacg tcgcctcgcg
     5101 gagcgtcggc ggcggtgctg gctcgccaga ccccgaaccg agctccccgg tggcgccacc
     5161 ctcgatcgtc gtgtcgccgg tgtcggtcga accgctggtg cccgtctcgc tgtccggtgg
     5221 ctccgcgtcg gtcccggtcg tgcgatcgcc agcgccagtg ctcgagctgt gctgcgtctc
     5281 tgtggccgcc gacgtcaggc tcgccagcgc aggcgcgtca ccgcagccgg ctgccagaac
     5341 cacgagcaga aacgagacgg tcgcggggcg cattcgaccc tcttatcagc gccgacgatt
     5401 tcgcgcatcc cggcacctcc ccaaaaagta accccggccg ccgtccggtc acccccaggg
     5461 gctcctctgg acgcgctggc gaatgccggt cgcccgccgc actgcccccg gcggccgcca
     5521 aagcctgccg gtgacgacgg ccaccctcgt cgatcgacgc caggtccctc gccattcccc
     5581 cggagcaccg cggtgatacg gtgttccatg ccccgccgag tcgctatcgc gcatgcctgc
     5641 ctgctcgcgt gcaccccgat gaccagggcg agcgacaccg gatccgacgc gatcagcctc
     5701 agcaccggct cggcccggac ggagcggtcg tctgggagcg cagcttcggc gacagcggga
     5761 cccactcggg gcacggcgtc gcggtcgacc aggatggtgg cgtggccatc gtcggcctgg
     5821 cgggcgcggg cgacccccca tcacgaaccc gtgggcgcgt cgctacagcg ccgacggcga
     5881 ggtcctgtgg acctttagcg aggacgcgcc cgccgccggc ttcagctatg cggtcaaggt
     5941 ggtgatcgac gaggacgccg ttcggcatcg ccgtcgcggg atcgagcgcc ggccccggga
     6001 acacccgcga cgcctggatc gtcaagctcg cgccatgatc gctcttacag gagctcgccg
     6061 aacagcgccg ggaacacccg cgagatcttg ctcttgagat attcgccgta ggtgccgtcg
     6121 aacaggtgca cgctgcggcc gtcccagcgc gaagcatggt cgtcgatggc ctcgcgcgca
     6181 ggcaagggcc gcacctgcgc gtcgaagtcg gggtcgaaga acagcggcag cgacagccgc
     6241 gagcgcccgc tggtgttgcg cacgcggtgc ggcgtcgagc gaaactcccc gccgctgagc
     6301 cgatcgagca tatcgccgat gttgcacacc agcgccccgg ggatcggcgg cgcgcagatc
     6361 cagccgcgtg gggtccggac ctcgagcccg ccgacctcgt cctgcaccag caatgtcaac
     6421 agtccataat cggtgtgctc cccgaccccc cagtcgctgt cgtcgccggg gggatagtga
     6481 aacacacgaa acagcaccgt cgggtccgcc gtgtagtgca gcttaaaata ttcggcgtcg
     6541 agccccagcg ccagcgcgac cccctccatc agcacatgcc cgacgccggt caatgcacac
     6601 atgacctcga ggacagcctc gcgcagcgcc ggcacctgcg ccgggaagag gttggccccg
     6661 tgcagcggca ccccggcgcg cacgcgcgga tggtcctgcg ccagctcggt cccgaagtac
     6721 agcccctcct tgcgatcggg ccgcccggac gtcagctcct caccgagcgc gaagtagccg
     6781 cgccaggccc gaccgccgcg ggccatcgcg atctcctggc gctgcgccag cggcagcgcg
     6841 aagaactcgc ggctcgccgc catcacccgc gagaacagct ccggcgcgac gccgtgcccg
     6901 accgcataaa agaacccgct gtcgcggcac gccgctgcga tctcgctggc gacccgcgcc
     6961 cgcgcgaccg catcaccatg cacgagcgga gagatgtcga cgacgggcag gctcacgggg
     7021 accgagggtc gttcatcatc acgcgcagat caaccgcctc gacgcatggt cgccgtgcgc
     7081 tgcgggagaa agcacggcag acctcctcgc cgtctatcac caggccggac gtggcctcgc
     7141 cgctcgagct tgggcgccct ggattcagga tcgaccggag cctatcattc gctgccgcct
     7201 gccaggctcg cggcgcaggg ctcgtgaagc cccac
//
LOCUS       DASWNJ010001731         1760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_66319, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001731 DASWNJ010000000
VERSION     DASWNJ010001731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66319"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="VGB85_28810"
     CDS             <1..1085
                     /locus_tag="VGB85_28810"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0138135.1"
                     /translation="QDRLRAQVEREPVQFFMHALGPLLDAAREEVAAFVGARPEDLVF
                     VRNATAGVNAVLSALPFAAGDELLTTDHVYGACRNALEHLCRRTGARLVIAPVPFPIS
                     GRDEVLAAIVAAVTPRTRLALIDHITSPTGLVFPIAEIVAALQARGVDTLVDGAHGPG
                     MVALDVAAIGAAYYTGNFHKWCCAPKGAAMLHVRRDRQADLHPSVISHGYTSSRGRSL
                     LWEEFDWTGTDDPTSWLCVPVAIRWLGQLLPGGWPELRARNHAMLLAGRDCIATALGI
                     PAPAPDDMLGNLAALPLPPGHHAPTSAFHHDPLQRALFERHHIEVPVPPWPGPPRRLI
                     RISAQVYNRHADYEALAAALRVELRG"
     gene            complement(1075..>1760)
                     /locus_tag="VGB85_28815"
     CDS             complement(1075..>1760)
                     /locus_tag="VGB85_28815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138136.1"
                     /translation="GPADTSGDTGSVVQTDTGSDDTTTSEPIGTTSGMTDTGGNDDTM
                     TGPDPTEGDVWHDLYQGCESSDTTWLAVGPMGKPLVCNTGMDKPAVQWAGWHDKVLHN
                     NKLEEFVLALVPDLGAGPHITGTYNGLMMPNAKTPHLRTTALCPVNQPQCQIQGNIRV
                     EQNAETVVGIEDFKLSSGQAFDIDIDLTLYPELRDGPFTVVFVVTSMTYCPGNRGLWL
                     APRIVEVNP"
BASE COUNT          214 a          606 c          647 g          293 t
ORIGIN      
        1 agcaggaccg cctgcgcgcg caggtcgagc gcgagccggt gcagttcttc atgcacgcgc
       61 tcgggccgct gctcgacgcc gcgcgcgagg aggtcgcggc cttcgtcggc gcgcgcccgg
      121 aggacctcgt ctttgtgcgc aacgcgaccg ccggggtcaa cgccgtgctg tcggcgttgc
      181 cgttcgcggc gggcgacgag ctgctgacca ccgaccacgt gtacggggcg tgtcgcaacg
      241 cgctcgagca cctgtgccgg cgcaccggcg cccgcctggt gatcgcgccg gtgcccttcc
      301 cgatctcggg gcgcgacgag gtgctcgcgg cgatcgtcgc cgcggtgacg ccgaggactc
      361 gcctcgcgct gatcgaccac atcacgagcc cgaccggcct ggtgttcccg atcgccgaga
      421 tcgtcgcggc cttgcaggcg cgcggggtcg acacgctggt cgacggggcg cacggacccg
      481 gcatggttgc gctcgatgtc gcggcgatcg gcgccgcgta ctacaccggc aattttcata
      541 aatggtgctg cgcgcccaag ggcgcggcga tgctgcatgt ccgccgcgat cgccaggccg
      601 acctgcaccc ctcggtgatc agccacggtt acacctcgtc gcgcggccgc tcgctgctgt
      661 gggaggagtt cgactggacc ggcaccgatg acccgacctc gtggctgtgc gtgccggtgg
      721 cgatccgctg gctcgggcag ctgttgccgg gcggctggcc ggagctgcgc gcgaggaacc
      781 acgcgatgct gctcgcgggt cgcgactgca tcgccacggc gctggggatc ccggcgcctg
      841 cgccggacga catgctcggc aacctcgcgg ccctgccctt gccgccgggt catcacgcgc
      901 cgacctccgc gtttcaccac gacccgctgc agcgcgcgct gttcgagcgc caccacatcg
      961 aggtcccggt gccgccgtgg ccggggccgc cgcgccggct gatccggatc tcggcgcagg
     1021 tctacaaccg ccacgccgac tacgaggcgc tcgcggctgc gctgcgggtc gagctcaggg
     1081 gttgacctcg acgatgcgcg gcgccagcca caggccgcgg ttgccggggc agtaggtcat
     1141 cgaggtcacc acgaagacga cggtgaaggg gccgtcgcgc agctccgggt agagcgtgag
     1201 gtcgatgtcg atgtcgaagg cctggccgga cgagagcttg aagtcctcga tgccgacgac
     1261 ggtctccgcg ttctgctcga cgcgaatgtt gccctggatc tggcactgtg gctggttgac
     1321 cgggcacagg gcggtcgtgc gcaggtgcgg cgtcttggcg ttcggcatca tcaggccgtt
     1381 gtacgtgccg gtgatgtgcg ggccggcgcc gaggtcgggg acgagcgcga gcacgaattc
     1441 ctcgagcttg ttgttgtgca acaccttgtc gtgccagccg gcccactgga ccgcgggctt
     1501 gtccatgccg gtgttgcata cgagcggctt gcccatcggg ccgacggcga gccaggtcgt
     1561 gtccgaggac tcgcagccct ggtacaggtc gtgccacacg tcgccctcgg tcgggtcggg
     1621 gccggtcatc gtgtcgtcgt taccgccggt gtccgtcatg cccgacgtcg tgccgatcgg
     1681 ctcgctggtg gtcgtgtcgt cgctgccggt gtcggtctgc acgacgctgc cggtgtctcc
     1741 ggaggtgtcc gcgggtccgg
//
LOCUS       DASWNJ010001732         1568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_278915, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001732 DASWNJ010000000
VERSION     DASWNJ010001732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278915"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VGB85_28820"
     CDS             complement(<1..389)
                     /locus_tag="VGB85_28820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138137.1"
                     /translation="MIDQTVLESCASGVISPEIALAQLLLSGSPPDPAELGRLARPGP
                     LQTLANLADRHRDQLERLSGLARAGFTPEGDDPVAGAAALFDRLATEAPEAAVAFYTF
                     GDGEALSDATAELAAVIRAWADPAGRRV"
     gene            complement(441..1415)
                     /locus_tag="VGB85_28825"
     CDS             complement(441..1415)
                     /locus_tag="VGB85_28825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0138138.1"
                     /translation="MLTVLSVGYPLAPVAADPAGGAEQVLAQLDRALVAAGHRSIVVA
                     PEGSSVAGELVAVPAVPASITDPHRDAAQAATRAAIAHVLATERVDIVHLHGLDFASY
                     LPADGPPVLVTLHMPVDWYPAGALRPTRAGVHLVPVSDNQAARAPAGLALLAPITNGV
                     DSATYAPSATKRDFALVIGRVAPEKGFHDAVAAARLADVPLVAAGRLFPYPAYYSYFA
                     ERLRPEFDVQRRFVGPVEGEAKRRLLARARCVLIPSTAPETSSLVAMEALASGTPVIA
                     YRAGALPGIVEDGVTRLSRRRRRGHGGGVAGSGQDQPCGLPRRRACPL"
BASE COUNT          230 a          543 c          612 g          183 t
ORIGIN      
        1 acgcgtcggc cggccgggtc ggcccaggcg cggatcaccg ccgcgagctc ggccgtcgcg
       61 tcggacagcg cctcgccgtc gccgaaggta tagaatgcga ctgccgcctc cggcgcttcg
      121 gtcgcgagcc ggtcgaacaa cgcggccgcg cccgccaccg ggtcatcgcc ctcgggtgtg
      181 aaaccagcgc gcgcaaggcc actcagccgc tcgagctggt cgcgatgccg gtcggcgagg
      241 ttggcgagcg tctgcaacgg ccccggccgg gcaagacggc ccaattcggc cgggtccggc
      301 gggctccccg agagcagcag ctgcgccagc gcgatctcgg gcgagatgac gccgcttgca
      361 cagctctcca gcacggtttg gtcgatcacg ccgcgagccg gtggtagagg tcgagatatg
      421 cccccgcggt ctcgcgcagg tcaaagcggg caagcgcggc ggcgcggcag gccgcagggt
      481 tgatcctgtc cacttccagc aacgccgccg ccatgcccgc gacgtcgtcg acgagaaagc
      541 cgggtgactc cgtcctcgac aatgccgggc agcgcgccgg cgcggtaggc gatgaccggc
      601 gtgcccgacg ccagcgcctc catcgccacc agcgacgagg tctcgggcgc ggtggagggg
      661 atcagcacgc aacgcgcgcg tgcgagcaag cggcgcttgg cctcgccctc gaccggcccg
      721 acgaagcggc gctgaacgtc gaactccggg cggagccgtt ctgcgaagta gctgtaatag
      781 gcgggatagg ggaacagccg gcccgcggcg accagcggga cgtccgcaag ccgggcggca
      841 gcgaccgcgt catggaaccc cttctcgggc gcgacgcggc ctatcaccag tgcgaaatcg
      901 cgcttggtgg cggagggcgc gtaggtggcc gagtcgaccc cgttggtgat cggggcgagg
      961 agcgcgagcc cggcgggcgc gcgcgcggcc tgattgtcgg atacgggcac caggtgcacg
     1021 cccgcgcgtg tcggacggag cgcgccggcg gggtaccagt cgaccggcat gtgcagcgtc
     1081 accaacaccg gcgggccgtc tgccgggagg tagctggcga agtcgaggcc gtggaggtgg
     1141 acgatgtcga cccgctcggt cgcgagcaca tgtgcgatcg cggcgcgcgt ggcggcctgc
     1201 gccgcgtcgc gatgggggtc cgtgatcgac gcaggaacgg ccggcacggc tacgagctcg
     1261 cccgcgacgc tgctgccctc cggcgcaacc acgatcgagc ggtggccggc ggcgaccagc
     1321 gcgcggtcga gctgtgccag cacctgctcc gcgccgccgg cgggatcggc ggcgacgggg
     1381 gcgagcgggt agccgacgga tagaacggtc agcacagggc gggctcgcgc gcggccagct
     1441 cgcaccagcg cgccgaggtc agcgcccagc cgaagcggtc gtacagcgcg cgctcgggcg
     1501 gcggcggggc ggcgaagtca taggtcggag catcgcgcca gcgttcgtgc gtcggtcgcc
     1561 agccgtgg
//
LOCUS       DASWNJ010001733         6423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_596351, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001733 DASWNJ010000000
VERSION     DASWNJ010001733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6423
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596351"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..1104
                     /locus_tag="VGB85_28830"
     CDS             181..1104
                     /locus_tag="VGB85_28830"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0138139.1"
                     /translation="MSGPEDRLVALIERVRSLADARTPAPIEAPGDDALGELEQEVNR
                     LIRATRTGLQERLLFLVGPVVMFRWVNAEGWPVEYVSPNIEALTGYPLADFATGRRPY
                     ASLIHKDDLPRVFEEVTSNSKGDVQWFVHQPYRMLRADGAVLWVADYTVILRDEAGTI
                     THYFGYIMDISEQIEQLGKLNAQEQMLLRLASPILQVGRGVLAMPILGVFAADGAARM
                     TDDLLAAISRSRAHTAILDLTGLQDIDAPTVELMLRTSRAVGLLGCRCVLSGISPRVA
                     TMVVQLGVNHQLTTVATLQDALELALRGATR"
     gene            complement(1120..2493)
                     /locus_tag="VGB85_28835"
     CDS             complement(1120..2493)
                     /locus_tag="VGB85_28835"
                     /inference="COORDINATES: protein motif:HMM:NF015657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138140.1"
                     /translation="MPASIKNLVLPALLLLACKDDDVPDEDTTGTSTGTTTASDTEPT
                     GGVQERSYWQDVAPIYYERCVTCHQSGGIAPFALDNYADAAGWAPASAAAVAERTMPP
                     WLVTDDGTCGDFRGSRALASDEIDTIVAWAAAGAPEGQPRDDLKTPALPGLDADLALQ
                     TPVFAPEIVGGPLAEFDEYRCFLVDPKIDHDVFLTGYQVTPGTPELVHHVLAMPVDPD
                     AIVEGGQTNAQRMQALDDESPDRLGWPCFSMAGEGVEAEGLPVTWAPGMGAVEYPANT
                     GVRLAAGRKVVIQIHYNLHDPELAGKSDSTKLELRLAESVEREGFFDMIDLFIDTLFD
                     AEPDSLAPGQESVKYDWSVPIGQHYLAGEGQVEVYGIFPHMHERGRRWQARLVDGDET
                     QCIGDVQSWDFDWQLYYFYDQPQIIRPGTRLEVTCDFDTRGAPGPVTPGWGTQNEMCL
                     AGLYVVP"
     gene            2704..3186
                     /locus_tag="VGB85_28840"
     CDS             2704..3186
                     /locus_tag="VGB85_28840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0138141.1"
                     /translation="MPIDIEDYYRRHGPMVLRRCRSLLRDEPQAVDAMHDVFVQLLTH
                     RDRLQDGAAASLLWTIATNLCLNKIRSRRRRPEDSRSELLAQIAAADDPEARGVARSL
                     LDKLFTGVPASTGTIAVLHLLDGMTLEEVAQTVGMSVSGVRKRLRGLRAGVSALAEVT
                     "
     gene            3183..3977
                     /locus_tag="VGB85_28845"
     CDS             3183..3977
                     /locus_tag="VGB85_28845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138142.1"
                     /translation="MNDRTDTPPEPTLPLERLALGELSAREVHGDDIEARLAAIAASN
                     AKILQNHPPERVRAEVERRVARARRSRSPRVLWLATPLAAAAVAALVWWPDPSIDPEA
                     LVAIAEPVTTVAGDPGDTRAKGIEPHLVLHRQEGERAVPLDPQAEARARDLLQISYVA
                     AGARHGVVLSIDGGGLVTLHHPPRADASTALLQTGAVPLPQSYELDAAPDFERFVLVT
                     ADSPLDPAQVLAAARSLAGGPGAASDPLPLPAGQRQRSFSIRKVAP"
     gene            3974..6118
                     /locus_tag="VGB85_28850"
     CDS             3974..6118
                     /locus_tag="VGB85_28850"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEY0138143.1"
                     /translation="MSLVSLLPWTLGALALATPPARAAEPVTTPATTQATPVSRRIAL
                     IVGANDGGNDRVRLRYAGSDARALARVLVEIGGLERADEVVLLDPTQAELQAGFTRAQ
                     AQVQRARAAGERVQFLFYYSGHADERGLLLGSDRVDYPRLRGLIHAVPAQVRLGMLDS
                     CSSGAFVRTKGGRMRPPLATAGDATVEGHAFLSSSSAEEAAQESDRIGGSFFTHFLIS
                     GLRGAADVNRDRKITLNEAYRFAFDETLAGTETTRHGPQHAVYDIQLVGSGDLVMTDL
                     RETSALLDIGPTVGGRVYIRDASGNLAAELYKGVGAGPVSIALEPGRYSVVVDDGQGL
                     TRTTLDVRAGRKNELTRRDLAEIAGESTIARGEQDASDPDAYRTIPISLGLVPPLTIN
                     NLEKRRKVINRLGINVIFGRAHRIHGVEVALGGNWTLEDVRGAQLALVANVVHGRVRG
                     LQSVVGGNIVGGDLVGFQGAAGFNFTRGNLAGVQASAGVNVVGGAVRGLQLTSGVNWA
                     HTVGGAQLAAINAARRVRGAQLGILNIGGKVRGAQLGLINVADDADAQVGLLGVSRKH
                     GVWLDVWTSDTAALNLAVKFRARHTYTFLAAGVHPSGPGNGFMSGIGFGGTVRLPHDL
                     AFDLDLATYAVQPRFDFNKSDPALLSTVRALLRWQVARRFAIFGGPTFNGYVDFSGDR
                     RRIGHGWVTGRVPIADSISARFWPGFALGIQI"
     gene            6236..>6423
                     /locus_tag="VGB85_28855"
     CDS             6236..>6423
                     /locus_tag="VGB85_28855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138144.1"
                     /translation="MQTSTDALSNARSMLHTKAIPLLALSLLAGACDAAIVAQANKDT
                     PPPSVQAEAAVEDEAKLEV"
BASE COUNT          901 a         2469 c         2125 g          928 t
ORIGIN      
        1 aggtccgcgt caacggccac gccctgcacg cgggcgacgg cgcggcgctg gcgagcgagc
       61 cggcggtgca gctcgagggc atcgacggcg gcgaggtgct cgtattcgat ctcgcctgat
      121 ccgtccggtc cccaaaaaca gaagcaggag cgctggaccg gatccactag agttcccgcg
      181 atgtccggcc cggaagatcg cctcgtcgcc ctgatcgaac gcgtgcgcag cctcgccgac
      241 gcgcgcacgc cggcgccgat tgaagcgccg ggggacgacg cgctcggtga gctcgagcag
      301 gaggtcaacc gcctgatccg cgcgacgcgt accggcctgc aggagcgcct gctgttcctc
      361 gtcgggccgg tcgtcatgtt ccggtgggtc aacgccgagg gctggccggt cgagtacgtc
      421 tcgcccaaca tcgaggcgct gaccggctac cccctcgccg acttcgccac gggccgccgc
      481 ccctacgcct ctctgatcca caaggacgac ctgccgcgcg tgttcgagga ggtcacgagc
      541 aacagcaagg gcgacgtgca gtggttcgta caccagccgt atcgcatgct gcgggccgac
      601 ggcgcggtgc tctgggtcgc cgactacacc gtgatcctgc gcgacgaggc cggcacgatc
      661 acgcattatt tcggctacat catggacatc agcgagcaga tcgagcagct cggcaagctc
      721 aatgctcagg agcagatgtt gctgcgcctc gccagcccga tccttcaggt cggccgcggc
      781 gtgctggcga tgccgatcct cggcgtgttc gcggccgacg gcgcggcgcg catgaccgac
      841 gacctgctcg ctgcgatctc ccgcagccgc gcacacacgg cgatcctcga cctcaccggc
      901 ttgcaggaca tcgacgcgcc gaccgtcgag ctgatgttgc ggaccagccg cgcggtcggc
      961 ctgctcggct gccgctgcgt gctctccggc atctctccgc gcgtcgccac catggtcgtg
     1021 cagctcggcg tcaaccatca gctgacgacc gtggcgacct tgcaggacgc gctcgagctg
     1081 gccctgcgcg gcgcgacccg gtgacgccgg ccccgagcct cagggcacga cgtagaggcc
     1141 cgcgaggcac atctcgttct gcgtacccca gccgggcgtg accggcccgg gggcgccgcg
     1201 ggtgtcgaag tcgcaggtga cctcgagccg cgtacccggg cggatgatct gcggctgatc
     1261 ataaaaatag tacagctgcc agtcgaagtc ccagctctgg acatcgccga tgcactgcgt
     1321 ctcgtcgccg tcgacgagcc gcgcctgcca ccggcgcccg cgctcgtgca tgtgcgggaa
     1381 gatgccatac acctcgacct ggccctcgcc ggcgaggtaa tgctggccga tcgggacgct
     1441 ccagtcgtac ttcacgctct cctgccccgg cgcgagcgaa tcgggctcgg cgtcgaacag
     1501 cgtgtcgatg aacaggtcga tcatgtcgaa gaagccctcg cgctcgaccg actccgcgag
     1561 gcgcagctcg agcttcgtgc tgtccgactt tcccgcgagc tccgggtcgt gcaagttgta
     1621 atggatctgg atcacgacct tgcggccggc cgcgagccgc accccggtgt tcgcgggata
     1681 ctcgaccgcg cccatgcccg gggcccaggt caccggcagg ccctcggcct cgacaccctc
     1741 cccggccatg ctgaagcacg gccagccgag ccggtcgggc gactcgtcgt cgagcgcctg
     1801 catgcgctgc gcgttggtct gtccgccctc gacgatcgcg tcggggtcga ccggcatcgc
     1861 cagcacgtga tgcaccagct cgggcgtacc gggcgtcacc tgatagccgg tcaggaacac
     1921 gtcgtgatcg atcttcgggt cgacgaggaa gcagcggtac tcgtcgaact ccgcgagcgg
     1981 tccaccgacg atctccggcg cgaacacggg ggtctgcagc gccaggtcgg cgtcgagccc
     2041 cggcagcgcg ggggtcttca agtcgtcgcg cggctggccc tcgggcgcgc ccgcggcggc
     2101 ccacgcgacg atcgtgtcga tctcgtcgct ggcgagcgcc cgcgagccgc ggaagtctcc
     2161 gcaggttccg tcatcggtca ccagccacgg cggcatcgtc cgctccgcga ccgcggcggc
     2221 gctggccggc gcccacccgg ccgcgtccgc gtagttgtcg agcgcgaacg gggcgatccc
     2281 gccggactga tggcaggtca cgcagcgctc gtagtagatc ggcgcgacat cttgccagta
     2341 gctgcgctcc tgcacgccgc cggtcggctc ggtgtcgctg gcggtcgtcg tgccggtcga
     2401 cgtgccggtc gtatcctcgt ccgggacgtc gtcgtccttg caggcgagca aaaggagagc
     2461 ggggagcacg aggttcttga tggaggcggg catggtcacc ggctgcaaca ccgcggcgtc
     2521 ccgttcgtgt caccacgact tgccggcgcg cgggcgcggg atcatcatcg ctggcgtgcc
     2581 gagccccgcg cagttccgcc cgcgccgcga aactcccctg cacgcccgag atccggcgaa
     2641 attcaccggt gacacttccg gcccgccgcg gtgttgcacg gtcaggaaga cccggggctg
     2701 accttgccca tcgacatcga ggattactac cggcgccacg gacccatggt cctgcgtcgc
     2761 tgtcggagcc tgctgcgcga cgagccgcag gcggtggatg ccatgcacga cgtgttcgta
     2821 cagctcttga cccaccgcga tcgcctgcag gacggggccg ccgcgagcct gctgtggacg
     2881 atcgccacca acctgtgtct caacaaaatt cgcagccgcc ggcgccggcc cgaggactcg
     2941 cgctccgagc tgctcgcgca gatcgccgcg gccgacgacc ccgaggcccg cggcgtcgcg
     3001 cgctccctgc tcgacaaact cttcaccggc gtacccgcct cgaccggcac gatcgccgtg
     3061 ctccacctgc tcgacggcat gaccctcgag gaggtcgcgc agaccgtcgg catgtcggtc
     3121 tcgggcgtgc gcaagcggct tcgggggctg cgcgccggcg tctcggcgct cgcggaggtc
     3181 acatgaacga tcgcaccgac acacccccgg agccgaccct gccgctcgag cgactcgcgc
     3241 tcggcgagct gtccgcgcgc gaggtccacg gcgacgacat cgaggcgcgc ctcgccgcga
     3301 tcgcggcctc gaacgcgaaa attttacaaa accatccacc ggagcgcgtg cgcgccgagg
     3361 tcgagcgccg cgtcgcccgg gcccgccgat cacgcagccc gcgggtgctg tggctggcga
     3421 ccccgctcgc cgccgcggcc gtcgctgcgc tggtgtggtg gcccgacccg tcgatcgacc
     3481 ccgaagcact cgtcgcgatc gccgagcccg tcaccaccgt cgccggcgat cccggggaca
     3541 cccgcgccaa gggcatcgag ccgcacctcg tgttgcatcg ccaggagggc gagcgcgccg
     3601 tcccgctcga tccccaagcg gaggcccgcg cgcgcgacct cctgcagatc agctacgtcg
     3661 ccgccggggc gcggcacggg gtcgtgctgt cgatcgacgg tggtgggctc gtcacgctgc
     3721 atcatccgcc gcgcgccgac gcctcgaccg cgctgctgca gaccggcgcc gtcccgctcc
     3781 cgcagtccta cgagctcgac gcggcccccg acttcgagcg cttcgtcctc gtcaccgccg
     3841 attcgccgct ggaccccgcg caggtgctcg ccgcggcgcg cagcctcgcg ggcggccccg
     3901 gtgccgcgag cgaccccttg ccgctgcctg ccggtcagcg ccagcgctcc ttctcgatcc
     3961 gaaaggtcgc gccatgagcc tcgtgtcact gctcccctgg accctcggcg ccctcgcgct
     4021 cgcgaccccg cccgcacgcg cggccgagcc tgtcaccacg cctgcgacca cgcaggccac
     4081 ccccgtgtcc cgaaggatcg cgctgatcgt cggcgccaac gacggcggca acgaccgcgt
     4141 gcgcctgcgc tacgccggca gcgacgcgcg cgcgctggcc cgcgtgctcg tcgagatcgg
     4201 cggcctcgag cgcgccgacg aggtcgtgct gctcgacccg acgcaggccg agctgcaggc
     4261 cggcttcacg cgcgcgcagg cccaggtgca gcgggcccgc gcggccggcg agcgggtcca
     4321 gttcctgttc tactactcgg gccacgccga cgagcgcggg cttttgctcg gctcggaccg
     4381 cgttgactat ccgcgcctgc gcggcctgat ccacgccgtg ccggcgcagg tgcggctcgg
     4441 catgctcgac tcgtgctcct cgggcgcgtt cgtgcggacc aagggcgggc gcatgcggcc
     4501 gccgctggcg accgccggcg acgcgacggt cgagggtcac gccttcctca gctcgagctc
     4561 cgccgaggag gccgcgcagg agtcggaccg catcggcggc tccttcttca cgcacttcct
     4621 gatctccggc ctgcgcggcg ccgccgacgt caaccgcgac cgcaagatca ccctcaacga
     4681 ggcctaccgc ttcgccttcg acgagaccct cgccggcacc gagaccacgc ggcacggccc
     4741 gcagcacgcc gtctacgaca tccagctggt cggcagcggc gacctcgtga tgaccgacct
     4801 gcgcgagacc tcggcgctgc tcgacatcgg tccgaccgtc ggcggtcgcg tgtacatccg
     4861 cgacgcctcg ggcaacctcg cggccgagtt atataaaggc gtcggcgccg gcccggtctc
     4921 gatcgcgctc gagccaggtc gttacagcgt cgtcgtcgac gacggccagg gcctgacgcg
     4981 cacgaccctc gacgtccgcg ccggccgcaa gaacgagctg acccgccgcg acctcgccga
     5041 gatcgccggt gagtcgacga tcgcccgcgg cgagcaggat gcctccgacc ccgatgcata
     5101 tcgcacgatc ccgatctcgc tcggcctcgt cccgccgctg acgatcaaca acctcgagaa
     5161 gcgccgcaag gtgatcaacc gcctcggcat caacgtgatc ttcggccgcg cccaccggat
     5221 ccacggcgtc gaggtcgcgc tcggcggcaa ctggaccctc gaggacgtgc gcggcgccca
     5281 gctcgcgctc gtcgccaacg tcgtccacgg ccgcgtgcgc ggcctgcagt ccgtcgtcgg
     5341 cggcaacatc gtcggcggcg acctcgtcgg cttccagggc gccgccggct tcaacttcac
     5401 gcggggcaac ctcgccggcg tgcaggccag cgccggggtc aacgtcgtcg gcggggccgt
     5461 gcgcggcctg cagctgacct cgggcgtcaa ctgggcccac accgtcggcg gcgcccagct
     5521 cgctgccatc aacgccgccc ggcgggtccg cggcgcccag ctcggcatcc tcaacatcgg
     5581 cggcaaggtc cgcggcgccc agctcggcct catcaacgtc gccgacgacg ccgacgccca
     5641 ggtcggcctg ctcggcgtct cgcgcaagca cggcgtgtgg ctcgacgtgt ggaccagcga
     5701 caccgccgcg ctcaacctcg cggtcaagtt cagggcgcgc cacacctaca ccttcctcgc
     5761 cgccggcgtg cacccgtccg gccccggcaa cggcttcatg tccggcatcg gcttcggcgg
     5821 caccgtgcga ctgcctcacg acctcgcctt cgacctcgac ctcgccacct acgccgtcca
     5881 gccgcgcttc gattttaata agagcgaccc ggccctgctg agcaccgtgc gcgccctgct
     5941 gcgctggcag gtcgcgcgcc gctttgcgat cttcggcggc ccgaccttca acggttacgt
     6001 cgacttctcc ggcgaccgcc gccggatcgg ccacggctgg gtcaccggcc gcgtcccgat
     6061 cgccgactcg atcagcgccc gcttttggcc cggcttcgcc ctcgggatcc agatctgatc
     6121 agtgccgcga cgtccgctcg aggatatacg cggcgagcaa ggcgccgcgc tcgccgccgg
     6181 ggcgcctcat gcaccggggt cgatcacccc cggtccacga cacccacccc ggaccttgca
     6241 aacatccacc gacgcgctgt ccaatgcgcg ttcgatgctg cacaccaagg ccatccctct
     6301 cctcgccctg tctctgctcg ccggtgcctg cgacgccgcg atcgtcgccc aggccaacaa
     6361 ggacaccccg ccgccgagcg tccaggccga ggcggccgtc gaggacgagg ccaagctcga
     6421 ggt
//
LOCUS       DASWNJ010001734         1505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_382283, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001734 DASWNJ010000000
VERSION     DASWNJ010001734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382283"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            267..860
                     /locus_tag="VGB85_28860"
     CDS             267..860
                     /locus_tag="VGB85_28860"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEY0138145.1"
                     /translation="MPRAPLTLCLLAIALACTPQIPPAPPESARAAANAGLGPGDALD
                     IRVFDEDRFDGEYQVGDDGQIDFPMVGGVEVRGKTKDEVARLLEHKLADGYLKNPQVS
                     VQVKQRGNREVSVLGQVNEAGSIDYRDGLSLVQAISLAGGLSAFAAPTRVKVTRRTGV
                     GEQTVTFEVSLSAIFNGKSEDLVLRPGDIVFVPESRI"
     gene            864..1493
                     /gene="hisB"
                     /locus_tag="VGB85_28865"
     CDS             864..1493
                     /gene="hisB"
                     /locus_tag="VGB85_28865"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEY0138146.1"
                     /translation="MPDAITQGREALVERSTRETQISVRLTLPTELPHPPHSVEIDTP
                     VPFLSHMLEAFARHGSLGLKVLARGDTQIDDHHTVEDTGLVLGSALDRALGLRAGIVR
                     FGHFSLCMDETLVDCALDLGGRPYLVFDLPAISGRRVGNFDCDLVREFFQALVVQARM
                     NLHLHLRAGGNVHHVVEAAFKGFARALRMAVAYDPALGMAIPSTKGNLV"
BASE COUNT          220 a          550 c          505 g          230 t
ORIGIN      
        1 ctcgccccgc gtctcccgat cgcgcccccc ggtctccgcg tcccgcaccg atcccggcgt
       61 cactcgagca cgcacccggg tccccaagtc ccgcaccgag cccgctgtca tccgagcatg
      121 cacccgggtc tccgagcccg ccatctcccg agcacgcacc cgagtccccg cgtcccgcac
      181 cgagcccgcc gtctcccgag caggtctccg ggtccccgcg tctcccgagc ccaagccgcg
      241 cggctcgtgg caaagtacat gcaacgatgc cccgcgcccc gctcacgctg tgcctgctgg
      301 cgatcgccct cgcatgcacc ccgcagatcc cgcccgcgcc gcccgagtcg gcgcgcgccg
      361 ctgcaaacgc gggcctgggt ccgggcgacg ccctcgacat tcgcgtgttc gacgaggacc
      421 gcttcgacgg ggagtaccag gtcggggacg acgggcagat cgactttccg atggtcggtg
      481 gggtcgaggt gcgtggcaag accaaggacg aggtggcgcg cctgctcgag cacaagctcg
      541 ccgacggcta tttgaagaat ccgcaggtca gcgtgcaggt caagcagcgc ggcaaccggg
      601 aggtcagcgt gctcgggcag gtcaacgagg ccgggtcgat cgactaccgc gacggcctga
      661 gcctggtcca ggcgatctcc ctggccggcg gactgagcgc cttcgcggcg ccgacgcggg
      721 tgaaggtgac gcggcggacc ggggtcggcg aacagacggt caccttcgag gtctcgctgt
      781 cggcgatctt caatggcaag tcggaggatc tggtattacg tccgggcgac atcgtgttcg
      841 tgcccgagtc gaggatctga cgcatgcctg acgcgatcac tcaaggccgc gaggcgctcg
      901 tcgagcgcag cacccgggag acgcagatct ccgtgcggct gacgctgccg actgaactac
      961 ctcatcctcc acactcggtg gagatcgaca cgccggtgcc gttcctctcg cacatgctcg
     1021 aggcgttcgc gcggcacggc tcgctcgggc tcaaggtgct cgcgcgcggc gacacgcaga
     1081 tcgacgacca ccacaccgtc gaggacaccg gcctcgtgct gggcagcgcg ctcgaccggg
     1141 cgctcgggct gcgcgccggc atcgtccgct tcgggcactt ctcgctgtgc atggacgaga
     1201 ccctcgtcga ctgcgcgctc gacctcggcg gccggccgta cctggtgttc gacctgcctg
     1261 cgatctccgg gcggcgcgtc ggcaacttcg actgcgacct cgtgcgcgag ttctttcagg
     1321 cgctggtcgt gcaggcgcgc atgaacttgc acctgcatct gcgggccggc ggcaacgtgc
     1381 atcacgtcgt cgaggcggcg ttcaagggct tcgcgcgggc gctgcggatg gcggtcgcgt
     1441 acgaccccgc gctcggcatg gcgatcccct cgaccaaggg caatctcgta tagaaggtga
     1501 gcccg
//
LOCUS       DASWNJ010001735         4113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_170850, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001735 DASWNJ010000000
VERSION     DASWNJ010001735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170850"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VGB85_28870"
     CDS             <1..285
                     /locus_tag="VGB85_28870"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0138147.1"
                     /translation="RGDIRLIILGEASCVGLAAGTVGVLLAYAASLGFDAVSASYVPD
                     FPYKPTTYFVFSADLVALALAFAAGFCVLGAYLPARRASRMDPAAVLTGR"
     gene            459..1016
                     /locus_tag="VGB85_28875"
     CDS             459..1016
                     /locus_tag="VGB85_28875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138148.1"
                     /translation="MGSCDTPYVGDLEALWLHVSDLPPGLTGRQLRARLFAVFATALQ
                     WRWDHGGYHFRFAELPLRPGRQPEAQARELLRAMFPAIRDEGLALVWCEPLGALDDFL
                     DRWLFFRRSRPYVAHDDVPAWMMPALREPWAACVRELVAADADARAAVLEGFCLTPDW
                     NDHGVALMLVAGARVGLFHVGLCTD"
     gene            complement(1074..1721)
                     /locus_tag="VGB85_28880"
     CDS             complement(1074..1721)
                     /locus_tag="VGB85_28880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138149.1"
                     /translation="MLRTYLCCAPLLVLAALPLGCGSKNDDTGDDTTSTTSNTTTSTT
                     STASTTSSTTTSTTTTVTTTTEPTTDPTEIGPECDDRSVFCMVAPGFEDTTTGGGSTA
                     GPDEPMCEPLLGELETSAQGLCSAGFAGIGLLSIDNLVGASKQCCYVYHCADPTQVCI
                     PLAEAGACAEEADLGDAAIAPQNACNTGIGSGIYPAPKGDACCSDFQCVCGPTGG"
     gene            complement(1816..>4113)
                     /locus_tag="VGB85_28885"
     CDS             complement(1816..>4113)
                     /locus_tag="VGB85_28885"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_function: GO:0003997 - acyl-CoA oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY0138150.1"
                     /translation="CYETTMLDSRPPISQDPSLLPFLPLLHVAWADGALEDDEQSTLR
                     RHLEAQPALSPSARAALLEWLDPARPPTPSTLVEWLEQLRQISSTVDPATVRTLGDLG
                     LAIAGDAADEHTVAALRTLAEALGLTRTPLSVILPAKPAAPAPTRTPVLDVAALRRLL
                     DGPEAPARDEIREYLADHRPPHDLATPELRVICHSWLRELGERGVGRFAFPGVTTDAS
                     DLSGFVARFETLALGDLSVLIKAGVQFGLFGGSIFHLGNEAQRKQYLPPTASLELLGC
                     FAMSETGHGSNVADLETTARYDPSTQEFEIDTPRDAARKDWIGGAATHARMATVFAQL
                     EVASERHGVHAFLVPIRDPAGALLPGVKATDAGEKMGLNGVDNGRLWFDKVRVPRAAM
                     LDRFATVTADGRYESAIPSPSRRFFTMLGTLVAGRVSIASAAVSVAKVGLTIAVRYAS
                     IRRQFGPSGAPETPILDYPTHQRRLLPALATTYALHFAARALQHAYVTAASGDREVET
                     LAAGLKAYASWHVTRTLQDCRECCGGQGYLAINRIPALLADADIFTTFEGDNTVLMQL
                     VARSLLTSFHRQFADARFVGLLRHLARQATTIILEKNPVVTRMTDPTHLRDREFQLAA
                     FRYRERRLLQTAAQRMKKRMDAGADGHTVVMAVQEHLVALAHAHVERVVLEAFDAGVR
                     ATTDASLARWLDHLCDLHALSRLAADMGWFVEDGYVDSWKARAVRKQVSALCMEIKDA
                     AVDLVDGFAIPEACLAPIAFAEGRS"
BASE COUNT          537 a         1279 c         1674 g          623 t
ORIGIN      
        1 cgcggcgaca tcaggctcat catcctcggg gaggcgagct gcgtcggcct cgcggccggc
       61 accgtcggcg tgctgctcgc ctacgcggcc agcctcggct tcgatgcggt gtccgccagc
      121 tacgtgcccg acttccccta caagccgacc acctacttcg tgttcagcgc ggacctcgtc
      181 gcgctcgcgc tcgcgttcgc ggccggcttc tgcgtgctcg gggcgtatct cccggcgcga
      241 cgggcttcgc gcatggatcc ggcggcggtg ctgaccgggc ggtgagcgtc gcgggtgcgg
      301 gcgctcgtgg tcgtgcgccc gacgaggagc gcgtgtggcc ggggtgaccg tcggtcgctt
      361 gtcggcggga gcaccgtcgc cgtaccctcg tcggcgcacc cgcgtgcacc catcagaaaa
      421 cccgcatgcc ggcgccgata gacatcgagc actctgacgt ggggtcctgc gatacgccgt
      481 acgtcggcga cctcgaggcg ctgtggctgc atgtgtcgga tctgccgccg gggctcacgg
      541 gacgccagct gcgtgcgagg ctgttcgcgg tgttcgccac ggcgctgcag tggcgctggg
      601 accacggtgg gtaccacttc cgcttcgccg agctgccgct gcggccgggc aggcagcccg
      661 aggcccaggc gcgcgagctc ctgcgcgcga tgttcccggc gatccgcgac gagggcctcg
      721 cgctggtctg gtgcgagccg ctgggcgcgc tcgacgactt cctcgaccgc tggttgttct
      781 ttcgtcgcag ccgcccatac gtggcccacg acgacgtgcc ggcgtggatg atgccggcgc
      841 tgcgcgagcc gtgggcggcg tgcgtgcgcg agctggtggc cgccgacgcc gatgcccgcg
      901 cggcggtgct cgagggcttt tgcctgacac ccgactggaa cgatcacggg gtcgccctca
      961 tgctcgtcgc cggcgcgcgc gtgggcctgt tccacgtcgg cttgtgtacc gactgacagc
     1021 ggtgcgcgcg cggcgatatg ccgaggtatc aggcggctcg tggggtccgc gcgtcagcca
     1081 ccggtggggc cgcaaacgca ctgaaagtcc gagcaacagg cgtcgccctt cggggccggg
     1141 tagatcccgg agccgatgcc ggtgttgcag gcgttctgcg gggcgatcgc ggcgtcgccg
     1201 aggtcggcct cctccgcgca tgcgccggcc tcggcgaggg ggatgcagac ctgggtcggg
     1261 tcggcgcagt ggtagacgta gcagcactgt ttgctcgcgc cgacgaggtt gtcgatcgac
     1321 agcaggccga tccccgcgaa gccggcggag cacaggccct gcgcgctggt ctcgagctcg
     1381 ccgagcaggg gctcgcacat cggctcgtcg gggccagcgg tgctcccacc gccggtcgtg
     1441 gtgtcctcga agccgggggc gaccatgcag aacacgctgc ggtcgtcgca ctccgggccg
     1501 atctcagtgg ggtccgtcgt gggctcggtg gtggtggtga cggtggtggt ggtcgacgtg
     1561 gtggtgctcg atgtggtcga cgcggtcgac gtggtcgacg tggtcgtgtt cgatgtggtc
     1621 gacgtcgtgt catcgccggt gtcgtcgttt ttcgagccgc acccgagggg cagcgcggcg
     1681 aggacgagca gaggcgcgca gcagaggtaa gttcgaagca tgcctgcttc atagcaggat
     1741 atggcgattg tcgagaaaag agcggcggcg ccgagcggcc ccgcgcacct gcatacgggg
     1801 atccggagat gcgagctagg agcgcccctc ggcgaacgcg atcggagcga ggcaagcttc
     1861 ggggatggcg aagccatcga cgaggtcgac cgcggcgtct ttgatctcca tgcagagggc
     1921 ggagacctgc ttgcgcacgg cgcgagcctt ccaggagtcg acgtagccgt cctcgacgaa
     1981 ccagcccatg tcggccgcga ggcgggacag ggcgtgcagg tcgcagaggt ggtcgagcca
     2041 gcgggcgagg gatgcgtcgg tggttgcgcg cacgccggcg tcgaaggcct cgaggacgac
     2101 gcgctcgacg tgcgcgtggg cgagggcgac gaggtgctcc tggaccgcca tcacgacggt
     2161 gtggccgtcg gcgccggcgt ccatgcgttt cttcatccgc tgcgcggcgg tctgcagcag
     2221 gcgtcgctcg cggtagcgga acgcggcgag ctggaactcg cggtcgcgca ggtgggtggg
     2281 gtcggtcatg cgcgtgacga ccgggttctt ctcgaggatg atggtcgtcg cctggcgggc
     2341 gaggtggcgc agcaggccga cgaagcgggc gtcggcgaac tggcggtgga aggaggtcag
     2401 caggctgcgg gcgaccagct gcatcagcac ggtgttgtcg ccctcgaagg tggtgaagat
     2461 gtcggcgtcg gcgagcagcg cggggatccg gttgatcgcc aggtagccct ggccgccgca
     2521 gcactcgcgg cagtcttgca gggtgcgcgt gacgtgccag ctggcgtagg ccttgagccc
     2581 ggcggcgagg gtctcgacct cgcggtcgcc cgaggcggcg gtgacgtagg cgtgttgcaa
     2641 cgcgcgcgcg gcgaagtgca gcgcgtaggt ggtggcgagc gcgggcagca ggcggcgctg
     2701 gtgggtcggg tagtcgagga tcggggtctc gggggcgccg gacgggccga actgacggcg
     2761 aatgctcgcg taacgaacgg cgatcgtcag gccgaccttg gcgacggaca cggccgcgct
     2821 ggcgatcgag acgcggccgg cgaccagcgt gccgagcatg gtgaagaagc ggcgcgaggg
     2881 gctggggatc gcgctctcgt agcgaccgtc ggcggtgacg gtggcgaagc ggtcgagcat
     2941 cgcggcgcgc gggacccgaa ctttgtcgaa ccacaggcgg ccgttgtcga cgccgttgag
     3001 gcccatcttc tcgccggcgt cggtcgcctt gacgccgggg agcagcgcgc ccgcggggtc
     3061 gcggatcggg acgaggaagg cgtgcacgcc gtggcgctcg ctggcgacct cgagctgggc
     3121 gaacaccgtg gccatgcgcg cgtgggtggc ggcgccgccg atccagtcct tgcgggccgc
     3181 gtcgcgcggg gtgtcgatct cgaactcctg cgtgctcggg tcgtagcgcg cggtggtctc
     3241 gaggtcggcg acgttggaac cgtggccggt ctcgctcatg gcgaagcagc cgagcaattc
     3301 caggctcgcg gtcggcggca ggtactgttt gcgctgggcc tcgttgccga ggtgaaagat
     3361 gctgccgccg aacaggccga actgcacgcc ggccttgatc agcacgctga ggtcgccgag
     3421 cgcgagggtc tcgaagcggg cgacgaagcc ggagaggtcg gaggcatcag tggtgacgcc
     3481 ggggaaggcg aagcggccga cgccgcgctc gccgagctcg cgtaaccagc tatggcagat
     3541 cacgcgcagc tcgggggtgg cgaggtcgtg cgggggcctg tggtcggcga ggtattcgcg
     3601 gatctcgtcg cgcgcggggg cctcggggcc gtcgagcagg cggcgcaggg cggcgacgtc
     3661 gagcacgggc gtgcgcgtcg gcgcgggcgc ggcgggcttc gcgggcagga tgacggagag
     3721 gggggtgcgg gtgaggccga gggcctccgc gagcgtgcgc agggcggcga cggtgtgttc
     3781 atcggcggcg tcgccagcga tcgccagacc gaggtcgccg agggtgcgca cggtcgcggg
     3841 gtcgacggtg ctcgagatct gtcgcagctg ctcgagccac tcgacgaggg tcgagggcgt
     3901 cggcggtcgg gccgggtcga gccactcgag cagggccgca cgggcggacg gcgacagggc
     3961 gggctgggcc tcgaggtgcc tgcgcagggt cgactgctcg tcgtcctcga gcgcgccgtc
     4021 ggcccaggcg acgtggagca gcgggaggaa gggcagcagc gacgggtcct gcgagatcgg
     4081 cgggcgcgag tcgagcatcg tggtttcgta gca
//
LOCUS       DASWNJ010001736         3278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_452782, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001736 DASWNJ010000000
VERSION     DASWNJ010001736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452782"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..419
                     /locus_tag="VGB85_28890"
     CDS             66..419
                     /locus_tag="VGB85_28890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138151.1"
                     /translation="MRTSIVTLSLSFALALSACAGGVSGSFKGNGVSAKPVPAINVKV
                     IANKAAMTGTYTELGIARGSAPTAQAAVGQAQRHCGDHGGTHLIMNTEPFQSGNSWKV
                     DATCATTGPAEGRAN"
     gene            complement(514..1608)
                     /locus_tag="VGB85_28895"
     CDS             complement(514..1608)
                     /locus_tag="VGB85_28895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0138152.1"
                     /translation="MAETPKPAKVATFGVRFESYDDFLVEYTDNLRRGFLLLPGVLNL
                     APEQVVRIKLSLPNRAILYLSGAVSDPGADGGKGTLIKLVAFTAEQEKILGLCVNSVL
                     VDPAPAATEAAPAKPINVLLVDDSPSVRTELGAALRARGINVTTADNGLLAMSAALKQ
                     EPDLVLTDVEMPEMDGWTLLRMARARKKLAHIPIVFLTALGDDMSRLQGYRMGIDDYL
                     PKTLPADEIVARIQGVLTRRAGNPNNSPESRGLRGDLHHVRLSSVLSFLEAEKKTGDL
                     RLDNGDDHAVLRIVHGILRDVKNLGEKKHAHDRVFQLLDWTSGQFEFFALPPGSEAEG
                     GPMEPTSVTYLLMEHARREDEAAEAARRRS"
     gene            1692..2969
                     /locus_tag="VGB85_28900"
     CDS             1692..2969
                     /locus_tag="VGB85_28900"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEY0138153.1"
                     /translation="MNKPRLGRSSPPLPASSRWGATGGILGTFGIVTTVLWVAVVFTS
                     VLFVPFALWCNFALARVTQLDPHANTSWLLLLSILVTLGLPLVLALWKHAGYPRRVWA
                     TLAWLPLVWNAGGLLLATQFAPEVLVNALRTQDTWAAQRLGDSHKLTRILSAIGHTLA
                     DQIDPPDDVPAVPAPPPAPVRAAVATEIAIPFSAAGNAILMSVELRGPAGRAELPYLF
                     DTGASFTTVNTKTAQLLGLEVPADAPTLQFNTASGPRESRMVYLPGLRLGDTVLEGLL
                     VSVCDGCVNERTAGLLGLNVMREFFVQMDYQAERMHLIPRQHEGRPNRAYDIGPTVKL
                     EVEGTPEIWLGRIRWVLLVENRGTVAIEGIIPEVQFSNGQRMRGAPIARIEPGGFGRS
                     LVEGKTQARGGPARTGGEKDELGFTLTIAEAYW"
     gene            2974..>3278
                     /locus_tag="VGB85_28905"
     CDS             2974..>3278
                     /locus_tag="VGB85_28905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138154.1"
                     /translation="MFYGILIIAAVLVFYLGNRRVQHMLSARNTEPRGRRLPQGKIRK
                     DPQFTAMVGRMVQRGLATPIAEASAEPVLIRGVLASADATLGGAPGRACVWQNRSGAP
                     "
BASE COUNT          464 a         1176 c         1113 g          525 t
ORIGIN      
        1 ccccgggcgc cgtctagccc ccatcgcccc gcgtcccctc gcccgtctgg agtctggagt
       61 ctctcatgcg cacctcgatc gtcaccctgt ccctgtcctt cgccctcgcc ctgtctgcct
      121 gcgcgggtgg cgtctccggt agcttcaagg gcaacggcgt gtccgccaag ccggtgcccg
      181 ccatcaacgt caaggtgatc gccaacaagg ccgcgatgac cggcacctat accgagctcg
      241 gcatcgccag gggctcggcc ccgaccgcgc aggcggccgt cggccaggcc cagcgccact
      301 gcggcgacca cggcggcacc cacctgatca tgaacaccga gccgttccag tccggtaaca
      361 gctggaaggt cgatgccacc tgcgccacca ccggcccggc cgagggccgc gcgaactgaa
      421 gcgccggctc ggaagcagca aactcaaaga ggcgcccccg cgggcgcctc tttcgtttaa
      481 ccattctggg acatgtccct cgggacatgt ctctcaggac cggcggcggg cggcctcggc
      541 ggcctcgtcc tcgcggcgcg cgtgctccat cagcaggtag gtgacggagg tcggctccat
      601 cggcccgccc tcggcctcgc tgccgggcgg cagggcgaag aactcgaact gcccgctggt
      661 ccagtcgagc agctggaaca cgcggtcgtg ggcgtgcttc ttttcgccga ggttcttcac
      721 gtcgcgcagg atgccgtgga cgatgcgcag cacggcgtgg tcgtcaccgt tgtcgaggcg
      781 caggtcgccg gtcttcttct cggcctcgag gaacgagagc acgctgctga ggcgcacgtg
      841 gtgcaggtcg ccacgcaggc cgcgggactc ggggctgttg ttcgggttgc cggcgcgacg
      901 cgtcagcacg ccctggatgc gcgcgacgat ctcatcggcg ggcaacgtct tgggcaggta
      961 atcgtcgatg cccatgcggt agccctgcag gcgcgacatg tcgtcgccga gggccgtcag
     1021 gaacacgatc gggatgtgcg cgagcttctt gcgggcgcgg gccattcgca gcagcgtcca
     1081 gccgtccatc tcgggcatct cgacgtcggt cagcacgagg tcgggctctt gcttgagcgc
     1141 ggcggacatc gccagcaggc cgttgtcggc ggtcgtgacg ttgatgccgc gggcccgcag
     1201 cgcagcgccg agctcggtgc gcacgctcgg cgagtcgtcg acgagcagca cgttgatcgg
     1261 cttggccggc gcggcctccg tcgccgccgg tgcggggtcg acgaggaccg agttcacgca
     1321 gagcccgagg atcttctcct gctcggccgt gaaggcgacg agcttgatga gggttccttt
     1381 gcccccatcg gcccccgggt cgctgacggc tccgctcaga tacaagatgg cccggttggg
     1441 caggctgagc ttgatgcgca cgacctgctc gggcgcgagg ttcaagacac ccggcaaaag
     1501 caagaagccg cgccgcaggt tgtccgtgta ctcgaccaga aagtcgtcgt aggattcgaa
     1561 gcgaacgccg aacgtggcga ccttggctgg tttgggcgtc tcggccattc ccggctcgta
     1621 tcctacggat tttgccgcgg ggatccaacg cccgtcttca ggcatctccg atttcggcta
     1681 gtatccccgc cgtgaacaaa ccgcgcctcg gacgctcgag tccacccctc cccgcgtcct
     1741 cgcggtgggg agcgaccggc ggcatcctcg gcaccttcgg catcgtcacc acggtgctgt
     1801 gggtcgcggt cgtgttcact tccgtactct tcgtgccgtt cgcgttgtgg tgcaactttg
     1861 cgctcgcgcg cgtcacccag ctcgaccccc acgccaacac ctcgtggctg ctgctcctca
     1921 gcatcctcgt caccctcggc ctgcccctcg tgctggccct gtggaagcac gcgggctatc
     1981 cgcggcgcgt ctgggcgacg ctggcgtggt tgccgctggt gtggaacgcc ggcggcctgc
     2041 tgctcgccac gcaattcgcg ccggaggttc tggtcaacgc gttgcgcacg caggacacgt
     2101 gggccgcgca gcgcctcggc gactcgcaca agctgacccg tatattgagc gcgatcgggc
     2161 acaccctcgc cgaccagatc gacccccccg acgatgtccc ggccgtgccg gcaccgccgc
     2221 ccgcgccggt tcgcgccgcg gtcgccacgg agatcgccat ccccttctcg gccgccggca
     2281 acgcgatcct catgagcgtc gagctgcgcg gtcccgccgg gcgcgccgag ctgccctacc
     2341 tgttcgacac cggcgcctcg ttcaccaccg tcaacaccaa gaccgcccag ctgctcggcc
     2401 tcgaggtgcc cgccgacgcg cccaccttgc agttcaacac cgccagcggg ccgcgcgaga
     2461 gccggatggt ctacctgccg ggcctgcgcc tcggcgacac ggtgctcgag gggctgctgg
     2521 tgtcggtgtg tgacggctgc gtcaacgagc ggaccgccgg gctgctgggg ctcaacgtga
     2581 tgcgcgagtt cttcgtgcag atggactacc aggccgagcg catgcacctg atcccgcgcc
     2641 agcacgaggg ccggcccaac cgcgcctacg acatcgggcc gacggtgaag ctcgaggtcg
     2701 agggcacccc ggagatctgg ctcggccgga tccggtgggt cttgctcgtc gagaaccgcg
     2761 ggaccgtggc gatcgagggg atcattccgg aggttcagtt ctcgaacggc cagcgcatgc
     2821 gcggggcacc gatcgcccgc atcgagcccg gcggcttcgg gcgctcgctc gtcgagggca
     2881 agacccaggc ccgcggcggc ccggcgcgca ccggcggcga gaaggacgag ctcggcttca
     2941 cgctgacgat cgccgaggcc tactggtgaa gcgatgttct acgggatcct gatcatcgcc
     3001 gcggtcctcg tgttctacct cggcaaccgg cgagtgcagc atatgctgag cgcgcgcaac
     3061 accgagcctc gcgggcgtcg cctgccgcag ggcaagatcc gcaaggaccc gcaattcacc
     3121 gcgatggtcg gccgcatggt ccagcgcggt ctggcgacgc cgatcgccga ggcgagcgcc
     3181 gagccggtgc tgatccgcgg ggtgctcgcg tccgcggacg ccaccctcgg cggcgcgccg
     3241 gggcgcgcgt gcgtgtggca aaaccgcagc ggggcccc
//
LOCUS       DASWNJ010001737         7292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_524884, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001737 DASWNJ010000000
VERSION     DASWNJ010001737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7292
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524884"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VGB85_28910"
     CDS             complement(<1..769)
                     /locus_tag="VGB85_28910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138155.1"
                     /translation="MPLPRLNGTLTPVGAPRYDQLFSDQLVTVMLAVGFDEYGANRPI
                     DDEVEAGLLARGFQADRDAALGRLAAGKRPAPQRPADHFIARGITRDDAGRPVDMVVS
                     LVRSRDGSAGGDAGSAYLEGLNRCEISMYSGHGRYGTGPDFDYNFTADLVDEAGAVIA
                     SYSEYRDLEQALHDRAQANKRSIYAEYRLLLRQGRLLIRRINSGNLVVNLRNYHANEF
                     GSHLMVDQLKTDANVRRMSKQRFDRKYRLWLFNGCRTH"
     gene            complement(845..1939)
                     /locus_tag="VGB85_28915"
     CDS             complement(845..1939)
                     /locus_tag="VGB85_28915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY0138156.1"
                     /translation="MSLLTIGLLLACGDAATGTSETASTGADTSTTTTPTSTGDTSTG
                     DTSTGGVTSTGDTSSTGATSTSTGTTSGPGTTTDPTTGPADPVALRALFINVGNASPQ
                     FGCFEYKLCRPQDVANVRAYIKTWQPDVIALSEVLRAGQLTDAVEGGPILPEGYTGVC
                     GESRDRGTGELAAWDADNASHEHECVAWKTARVELVPNGTASVYGAEGCNYDFTGFRA
                     RLRLDGAHELAVVAVHPDSQETECRKQEIAGYWSELAVGERVLIGGDWNSDSDDELQR
                     PRGFDIAYLRGQHWDIALHDGEYSAVYGFGLQKKQFDHAFTNFGAAVTDALPFGSALG
                     GHDDHPRADGGDGLDHRQLLVDLAFLPDAP"
     gene            2007..2666
                     /locus_tag="VGB85_28920"
     CDS             2007..2666
                     /locus_tag="VGB85_28920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138157.1"
                     /translation="MARARRAIALTVAVALAPVQTLAQTPTAEVAPAPATGTEAAPGS
                     PAASIPATPRESPGTFTTQDGWTTPKPGPTTSGTPPERRWQIRQAPLAGSESSWGPPG
                     GQMPEYAEFVETPEAKGPPPKGTPRIVAGAILGPLGLALAVAGIVGATTDMLGEQKRL
                     SVPMVGVGVVASAIGWGLFADGILRRKKFMRWQAKGGQLTVAPALVPGRLAGVSLGLR
                     F"
     gene            complement(2671..3339)
                     /locus_tag="VGB85_28925"
     CDS             complement(2671..3339)
                     /locus_tag="VGB85_28925"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0138158.1"
                     /translation="MTLLPCVEVEARGPATASVIWLHGLGADGHDFESIVPHLKLSHA
                     RFVFPNAPAIPITINGGFVMPGWYDIRSLELSPHVKDREDPAQIRKSTAAIEAIIARE
                     VERGIPHDRIVLAGFSQGGAMALYVAPRHAHKLAGFMVLSAYELLVGSGGEHEANTST
                     PALFCHGRNDAMVPMFAGQAACESARKSGREVRWYEFPIGHEVSMPEIQTIGSWLRER
                     LPPV"
     gene            complement(3374..4303)
                     /locus_tag="VGB85_28930"
     CDS             complement(3374..4303)
                     /locus_tag="VGB85_28930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138159.1"
                     /translation="MLRWPRLASLSVLALFACTAPPPDAPAPVAPAPAIPATPAPASE
                     PPPTPEPPPTPESPPTGEPMSSWWCICYSRVAKVEAEKVTACRKTEAECHGLERAVAA
                     GKSGMVARSLSRPCIELTAAHPGDVHGGRQAWSPSKKPGSWMSLGFCRLPGEGELVQN
                     DDGFKVMLDEKIGEVTVSSTTDEVVQLLGEPKKKGRIQMSEASGDDLQTWSYPAHGLS
                     IEMAIDDRKTGKGSVAAISMKAPATLKTRLGIGIGSTRKEVLDAYGKLRDPEFPSGTD
                     EEVFLAGSIYGGVFFTFNKKDKVSEIFVGAGAE"
     gene            complement(4472..5809)
                     /locus_tag="VGB85_28935"
     CDS             complement(4472..5809)
                     /locus_tag="VGB85_28935"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY0138160.1"
                     /translation="MHAPTPRGLAIQILRQLDQREGFSNRVLAEHLLAWSADLARRDR
                     GLVTLLVYGVLRHRARLDAMIDAFADKPSRLSARLRELLRLAVFELRELGRPAHVAIS
                     QAQTLRQEFDPTGALRGLVQAVLAAVDREGAALDEKWASGKPLEALALRWSIPRWLAG
                     RMIKQLGPERALARARAVAEPPPVDLRVDLGRTTAEAVVAALTEGDSRVTIEPVPGQP
                     QALRVRSGGELTQHPLYHAGHFAVQGLGAQQAALALAPCPEEHILDACCGMGTKTLHL
                     AELMHRRGKIVAVDASVERLDAHTEMRQRGGLVDVAELELQLFHGDLTQTLPGVDDDL
                     YDAILLDAPCTGLGNLGRHPELRWTSRYEDIAACAVLQRALLERCLTRLRPTGRLVYA
                     VCSLEPEEGPLLVRAVAEARGLQLELEQSWTPEEHATDGFYLARLRPGNTTAG"
     gene            5873..6391
                     /locus_tag="VGB85_28940"
     CDS             5873..6391
                     /locus_tag="VGB85_28940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138161.1"
                     /translation="MTANSPRMALSTCLVGWVMSVVMVTACDDPPPAPQPDNELLTQV
                     QAANYEEWARPAGYDEPRPSNSPHGPAVEVFTNAVVEEAVANEDGLGRTAWPEDATVV
                     LVGRASVLVTEASQISVMQKRHGVWYWEQYQADDLSRPRFSGRPDICLGCHSSGQDFI
                     RSFGLPKPVVEE"
     gene            6474..>7292
                     /locus_tag="VGB85_28945"
     CDS             6474..>7292
                     /locus_tag="VGB85_28945"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY0138162.1"
                     /translation="MTTNSLHAHVPGSRRWWAVAGLATAGFLAAPMTTAMAAAVCYKL
                     PFKNPNLADGWGSLCCGRTSPHRGVDFPQASGTSIPAVADGKVVVNTYSSCLGNVVVL
                     EHADGMYSGYAHLVSPSPLKKGAVVALGKSVGKVGDSGTCSNGAHLHLTMSPQVGGWA
                     SGTTVDPYKYIQSHLTCNTNPKGKLLSVDCEQIYGWTQDPDTPKEDISARLFFDGPSS
                     DPDAAHKTLVADLESDLLCDAIDSCDHGFTMLAPYSLFDGADHDVYAYGIDSGTN"
BASE COUNT         1026 a         2323 c         2820 g         1123 t
ORIGIN      
        1 cgtgggtgcg gcagccgttg aacagccaca ggcgatactt gcgatcgaag cgctgcttcg
       61 acatgcggcg cacgttggcg tcggtcttga gctggtcgac catcaggtgc gagccgaact
      121 cgttggcgtg atagttgcgc aggttgacga cgaggttgcc gctgttgatc cggcgaatga
      181 ggaggcgacc ctggcgcagc agcaggcgat actcggcgta gatcgaccgc ttgttggcct
      241 gcgcgcggtc gtgcagggct tgctcgaggt cgcgatattc gctgtagctg gcgatcacgg
      301 cgccggcctc gtcgacgagg tcggcggtga agttgtagtc gaagtcgggg ccggtgccgt
      361 aacgaccatg cccgctgtac atcgagatct cgcagcgatt gaggccctcg aggtacgcgg
      421 agccggcgtc accaccggcg ctgccgtccc gcgagcgcac cagcgagacg accatgtcga
      481 ccgggcggcc ggcgtcgtcc ctggtgatcc cacgcgcgat gaagtggtcg gcggggcgct
      541 gcggtgcggg ccgcttgccg gccgcgaggc gccccagcgc ggcgtcgcgg tcggcctgga
      601 agccacgcgc gagcagcccg gcctcgacct cgtcgtcgat cggccggttg gcgccgtact
      661 cgtcgaagcc gaccgcgagc atgaccgtga cgagctgatc gctgaacagc tggtcgtacc
      721 gcggggcgcc gaccggcgtg agggtcccgt tgaggcgcgg caggggcatc gtgtctccgt
      781 gcgggcgcgg cccgcccggc gagggtaact catctcgagg ccgcgaggtc gggcgcgcag
      841 gcgctcacgg agcgtcaggc aggaaggcga ggtcgacgag cagctggcga tggtcgagac
      901 cgtcaccacc gtcggcgcgc ggatgatcgt cgtggccgcc gagcgcggag ccgaaaggca
      961 gcgcgtcggt gaccgcggcg ccgaagttgg taaaggcgtg gtcgaactgc ttcttttgca
     1021 aaccgaagcc gtaaacggcc gaatactcgc cgtcgtgcag cgcgatgtcc cagtgctggc
     1081 cacgcaggta ggcgatgtcg aagccgcgcg ggcgctggag ctcgtcgtca ctatcgctgt
     1141 tccagtcgcc accgatcagc acgcgctcgc cgacggccag ctcggaccag tatcctgcga
     1201 tctcctgctt gcggcactcg gtctcctgcg agtcggggtg gacggcgacg acggcgagct
     1261 cgtgcgcgcc gtcgaggcgc aggcgcgcgc gaaagccggt gaagtcgtag ttgcaacctt
     1321 cggcgccgta tacggaggcg gtgccgttcg ggacgagctc gacgcgggcg gtcttccagg
     1381 cgacgcactc gtgctcgtgc gaggcgttgt cggcgtccca cgctgccagc tcgccggtgc
     1441 cgcggtcgcg gctctcgccg cacactcctg tgtaaccctc gggtaagatc gggccgccct
     1501 cgaccgcgtc cgtcagctgg ccggcgcgta acacctcgct caatgcgatg acgtcgggct
     1561 gccaggtttt aatataggcg cgcacgttgg cgacgtcctg cggccggcac agcttgtatt
     1621 cgaagcagcc gaactgcggc gaggcgttgc cgacgttgat aaagagggcg cgcagcgcga
     1681 cgggatcggc agggccggtg gttggatcgg tggtggtgcc gggtccggag gtggtgcccg
     1741 tgctcgtgct ggtggcgccg gtgctgctgg tgtcgccggt gctggtgacg ccgccggtgc
     1801 tggtgtcgcc agtgctggtg tcgccggtgc tcgtcggcgt ggtggtcgtg gatgtgtcgg
     1861 cgccggtgga ggcggtctcg ctcgtgccgg tggcagcgtc gccgcaggcg aggaggaggc
     1921 cgatcgtgag cagggacatg cgggccgggt gcatcgcggc gcggacgcta gcacgcaggc
     1981 gcgcgagatg tgcgaggatg cgcgcgatgg cgagggcacg acgagcgatc gctctgaccg
     2041 tggcggtcgc gctggcgccg gtgcagacgt tggcccagac gcctaccgcg gaagttgcac
     2101 ccgcgcccgc gacgggcacg gaggcggcgc cgggcagccc cgccgcgagc atcccggcga
     2161 cgccgcgcga gtcgccgggc acgttcacga cgcaggacgg ctggacgacg ccgaagcccg
     2221 ggccgacgac ctccggcacg ccaccggagc gtcgctggca gatccgtcag gcgccgctgg
     2281 cgggctcgga gtcgtcgtgg gggccgcctg gcgggcagat gccggagtac gcggaatttg
     2341 tggaaacgcc ggaggcgaag gggccgccgc cgaaggggac gccgcggatc gtcgcagggg
     2401 cgatcctcgg gccgctgggg ctcgcgctgg cggtcgcggg gatcgtcggc gcgacgaccg
     2461 atatgctcgg cgaacaaaag cggctgtcgg tgccgatggt cggcgtcggc gtggtcgcgt
     2521 cggcgatcgg ctgggggctg ttcgcggacg ggatccttcg gcgcaagaag ttcatgcgct
     2581 ggcaggccaa gggcggacag ctgacggtag cgccggcgct ggtgccgggc cgactcgcgg
     2641 gtgtcagcct cggtcttcgc ttttagaaat ttagaccggc ggcaggcgct cgcgcagcca
     2701 gctgccgatc gtctggatct ccggcatcga gacctcgtgg ccgatcggga actcgtacca
     2761 gcggacttcg cggccgctct tgcgcgccga ctcgcacgcg gcctggccgg cgaacatcgg
     2821 gaccatcgcg tcgttgcggc cgtggcaaaa cagggccggg gtgctcgtgt tggcctcgtg
     2881 ctcgccgccg cttccgacca gcagctcgta ggcgctgagg accatgaagc cggcgagctt
     2941 gtgggcgtgg cgcggcgcga cgtacagcgc catcgcgccg ccctgactga agccggcgag
     3001 cacgatgcgg tcgtgcggga tgccgcgctc gacctcgcgg gcgatgatcg cctcgatcgc
     3061 ggcggtcgac ttgcggatct gggccgggtc ctcgcggtcc ttgacgtgcg ggctgagctc
     3121 gaggctgcga atgtcgtacc agcccggcat cacgaagccg ccgttgatcg tgatcgggat
     3181 cgcaggggcg ttcgggaaca cgaagcgggc gtgcgagagt ttgaggtgcg ggacgatcga
     3241 ctcgaagtcg tgaccgtcgg cgccgaggcc gtggagccag atcacgctgg cagtggcggg
     3301 tccgcgggcc tcgacttcga cgcaggggag cagggtcatc gcgggcgcag actaccaccc
     3361 cgcgctcgcc cggtcactcg gcgccagcgc cgacgaagat ctcgctgacc ttgtccttct
     3421 tattaaaggt gaagaacacg ccgccgtaga tcgacccggc gaggaacact tcctcgtcgg
     3481 tgcccgaggg aaactccggg tcgcgcaatt ttccataggc atcgagcacc tccttgcgtg
     3541 tggagccgat gccgatgccg aggcgggtct tgagcgtcgc gggcgccttc atcgagatcg
     3601 cggcgaccga gcccttgccg gtcttgcggt cgtcgatcgc catctcgatc gagaggccgt
     3661 gggccgggta ggaccaggtc tggaggtcgt cgccgctggc ctcggacatc tgaatgcggc
     3721 ccttcttctt cggctcaccg agcagctgca cgacctcgtc ggtggtcgag ctgaccgtga
     3781 cctcgccgat cttctcgtcc agcatcacct tgaagccgtc gtcgttttgt acgagctcgc
     3841 cctcgccagg caagcgacaa aagccgaggc tcatccacga gcccggcttc ttcgaggggc
     3901 tccaggcctg gcgtccgccg tgcacgtcgc cggggtgggc cgccgtcagc tcgatgcacg
     3961 gccgtgacag cgagcgcgcg accatgccgc tcttgccggc cgcgacggcg cgctcgaggc
     4021 cgtggcactc ggcctcggtc ttgcgacacg cggtgacctt ctcggcctcg accttggcga
     4081 cccgcgagta acagatgcac caccagctcg acatcggctc gccggtcggg ggcgattcgg
     4141 gtgtcggggg cggttcgggc gtcggtggtg gctcgctcgc cggggccggg gtcgcgggga
     4201 tggcgggcgc tggcgcgacc ggtgcaggtg cgtcgggcgg gggcgcggtg caggcgaaca
     4261 atgcgagcac tgagagcgac gcgaggcgag gccagcggag catggcggcg agtgtatcag
     4321 aggggtcgcg gagcccgtgg cgctcgccgg cgcgcgctcg ttgcaggagc agtacgcgtc
     4381 gctcaaggtg ctgcgtcggt cctggcaccg ctcgcgtcat gagcattcga tcgtggtcgt
     4441 gcatcgatgg cgaccgaaca atgtacgccg gctatccggc ggtcgtattc ccggggcgca
     4501 ggcgggcgag gtaaaaaccg tcggtcgcgt gctcctcggg ggtccaggac tgctcgagct
     4561 cgagctgcag gccccgcgcc tcggcgacgg cgcggacgag cagtgggccc tcctccggct
     4621 cgaggctgca cacggcgtag acgaggcggc cggtcgggcg caggcgggtc aggcagcgct
     4681 cgagcagcgc gcgttgcagc acggcgcagg cggcgatgtc ctcgtagcgg ctggtccagc
     4741 gcagctcggg gtggcggccg aggttgccga ggccggtgca gggggcgtcg agcaggatcg
     4801 cgtcataaag gtcgtcgtcg acgccgggca gggtctgcgt gaggtcgccg tggaagagtt
     4861 gcagctcgag ctcggcgacg tcgacgaggc cgccgcgttg gcgcatttca gtatgggcgt
     4921 cgaggcgctc gacgctggcg tcgaccgcga cgatcttacc tcggcggtgc atcagctcgg
     4981 cgaggtggag ggtcttggtg cccatgccgc agcaggcgtc gaggatatgt tcttcgggac
     5041 atggcgcgag ggccagggcg gcctgctggg cgccgaggcc ctgcacggcg aagtggccgg
     5101 cgtggtagag cgggtgctgg gtcagctcgc cgccggagcg cacgcgcagg gcctggggct
     5161 ggccgggcac gggctcgatg gtgacgcggc tgtcgccctc ggtcagcgcg gcgacgacgg
     5221 cctcggcggt ggtgcggccg aggtcgacgc gcaggtcgac gggaggaggc tcggcgacgg
     5281 cgcgggcgcg ggcgagcgcg cgctccgggc cgagctgctt gatcatgcgt ccggcgagcc
     5341 agcgggggat cgaccagcgc agggccaggg cctcgagcgg cttgccgctg gcccacttct
     5401 cgtcgagcgc ggcgccctcg cggtcgaccg cggcgagcac ggcctgcacg agcccgcgca
     5461 gggccccggt cgggtcgaac tcctggcgca gcgtctgggc ctgcgagatc gccacgtggg
     5521 ccgggcggcc gagctcgcgc agctcgaaca ccgcgaggcg cagcagctcg cgcaggcggg
     5581 cgctcaggcg cgagggcttg tcggcgaagg cgtcgatcat cgcgtcgagg cgggcccggt
     5641 ggcgcagcac gccgtagacc agcagggtga cgaggccgcg gtcgcggcgg gcgaggtcgg
     5701 cgctccaggc gaggaggtgc tcggcgagca cgcggttcga aaaaccctcg cgctggtcga
     5761 gctgacgcag gatctggatg gcgaggccgc ggggggtcgg ggcgtgcacg ggccggcttc
     5821 ttagcacgcg cggcatggcg tgacaccgcc cgttcttgtt atcctccgcg agatgaccgc
     5881 gaactcgccg cgcatggcgc tgtcgacctg cctcgtcggt tgggtcatgt ccgtggtcat
     5941 ggtcacggcc tgcgacgacc cgccgccggc gccgcagccg gacaacgagc tgctgacgca
     6001 ggtgcaggcg gcgaattacg aggagtgggc gcggccggcc ggctacgacg agccgcggcc
     6061 gagcaacagc ccgcacggtc cggcggtcga ggtgttcacg aacgccgtgg tcgaggaggc
     6121 ggtcgccaac gaggacgggc tcgggcggac ggcgtggccg gaggacgcga cggtggtgct
     6181 cgtcggtcgt gcgagcgtgc tggtgaccga ggcctcgcag atctccgtga tgcagaagcg
     6241 gcacggggtc tggtactggg agcagtatca ggccgacgac ctgtcgcggc cgcgcttctc
     6301 cgggcggccg gacatctgcc tcggctgtca cagcagcggg caggacttca tccgcagctt
     6361 cgggctgccc aagccggtcg tggaggagta gtcgcggtga tgcttcgagc ccccggtgtg
     6421 ggggctcggg cgtggagttg acgcgtttgc gggcgtcccg caggcttgcg cgcgtgacga
     6481 cgaattcgct gcatgcacac gttcccggct cgcgtcgctg gtgggcggtc gcggggctgg
     6541 ccacggcggg ctttttggcg gcgccgatga cgacggcgat ggcggcggcg gtctgttaca
     6601 agctgccgtt caagaacccg aacctggccg acggctgggg ttcgctgtgt tgcggtcgca
     6661 ccagcccgca ccgcggcgtc gacttcccgc aggccagcgg cacgtcgatc cccgcggtcg
     6721 ccgacggcaa ggtggtcgtc aatacgtact cgagctgcct cggcaatgtg gtggtgctcg
     6781 agcacgcgga cggcatgtat tcgggttatg cgcacctggt ctcgccctcg ccgctgaaga
     6841 agggcgccgt ggtggcgctc ggcaagagcg tcggcaaggt cggcgacagc ggcacgtgct
     6901 cgaacggggc gcatctgcac ctgacgatga gcccgcaagt cggtggctgg gcgtcgggca
     6961 cgacggtcga cccctacaaa tatatccaga gtcacctgac gtgtaacacc aacccgaagg
     7021 gcaagctgct gtcggtcgac tgcgagcaga tctacgggtg gacgcaggac ccggacacgc
     7081 cgaaggagga catctcggcg cggctgttct tcgacggccc ttcgtccgac ccggacgcgg
     7141 cgcacaagac cctcgtcgcc gacctcgaga gcgacctgct gtgcgacgcg atcgactcgt
     7201 gtgaccacgg cttcacgatg ctcgccccgt acagcctgtt cgacggcgcc gatcacgatg
     7261 tctacgcata tggcatcgac agcggcacga ac
//
LOCUS       DASWNJ010001738         2791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_524895, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001738 DASWNJ010000000
VERSION     DASWNJ010001738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524895"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="VGB85_28950"
     CDS             complement(<1..1049)
                     /locus_tag="VGB85_28950"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HEY0138163.1"
                     /translation="MKTVIGLFDDFSAAQAAVEALVARNFNRNDISIAANQSGTAAGA
                     ASADSDGAHGDDRSHAMQEGAKSGAGAGAVVGTGIGGTVGLLAGLGVILIPGIGPIIA
                     AGPLVAALTGAGVGAAAGAAVGGLVGALTRLGVPEHDAHFYAESVRRGGALVMVRAED
                     DLANDAAEILGDAGAVDVDERRSHFEQQGFAAHDTSAPHYTAEQIAQERETYRQSQQS
                     QQVQQNRGPQLVEQAEATVPVIEETLTVGKRRVERGGIRMYTAVSETPVQEQVTLTQQ
                     HATIERKAVDRPVSATDTQAFQEQTFEVREFAEQAVVAKEARVVEEVTIRKEVAAHTE
                     VIQDTVRRTDVAVEEV"
     gene            complement(1046..1801)
                     /locus_tag="VGB85_28955"
     CDS             complement(1046..1801)
                     /locus_tag="VGB85_28955"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HEY0138164.1"
                     /translation="MNTPAILLVTDRDGVRGFIDVGTDGWTPDRVDTLIQLSDGGNVR
                     VPTSLLTQLDDSNYFLSCSIADLLSPETMATSPAPPRGTHTSGSTGPVVSDDVIIAEV
                     SVSSVVATVPVVQETFEVHKQVREAGSVRVNKHVHVRTEHVEVPLRSENIEITRVPIN
                     RVVDGPVPVRHEGDTTIYPVLEEVLVLEKRLVLREELHLRVRQSERVETHDIELRSEE
                     VTTERSAPPPPPITPPSTPTIPPPPTIKRGETP"
     gene            complement(1798..1920)
                     /locus_tag="VGB85_28960"
     CDS             complement(1798..1920)
                     /locus_tag="VGB85_28960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138165.1"
                     /translation="MKIYDRPGALALAARPISLIALALISLAIASFVYYKYYAV"
     gene            2433..>2791
                     /locus_tag="VGB85_28965"
     CDS             2433..>2791
                     /locus_tag="VGB85_28965"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0138166.1"
                     /translation="MPSPAPTFSARSYAFAPYAPCHNWIAGQWTPAASGETLPVHNPR
                     HGQVLGHVALSGAADVARAVAAAKAAFPGWRDTPLKERVQVLFRLKALMERDIEELTW
                     LVSHENGKVYAEAKGDVL"
BASE COUNT          404 a          975 c          962 g          450 t
ORIGIN      
        1 acctcctcga ccgcgacgtc ggtgcgacgc acggtgtcct ggatgacctc ggtgtgggcc
       61 gcgacctcct tgcggatcgt gacctcctcg acgacgcgcg cctccttggc gacgaccgcc
      121 tgctcggcga actcgcggac ctcgaaggtc tgctcctgga acgcctgcgt gtcggtcgcg
      181 ctgaccgggc ggtcgacggc cttgcgctcg atcgtcgcgt gctgctgcgt cagggtcacc
      241 tgctcctgga ccggcgtctc gctgaccgcc gtgtacatgc ggatcccgcc gcgctcgacg
      301 cgacgcttgc cgaccgtcag cgtctcctcg atcaccggga ccgtcgcctc ggcctgctcg
      361 acgagctgcg ggccccggtt ctgctggacc tgctggctct gctggctctg gcggtaggtc
      421 tcgcgctcct gggcgatctg ctcggccgtg tagtgcggcg cgctggtgtc gtgggccgcg
      481 aagccctgct gctcgaagtg cgagcgacgc tcgtcgacat cgaccgcgcc ggcgtcaccg
      541 aggatctccg ccgcgtcgtt cgcgaggtcg tcctcggcgc ggaccatcac cagcgcgccg
      601 ccgcggcgca ccgactcggc gtagaagtgg gcgtcgtgct cgggcacgcc gaggcgggtc
      661 agcgcgccga cgaggccacc gaccgcggcg cctgccgcag caccgacgcc ggcgccggtc
      721 agcgcggcga ccagcggacc ggccgcgatg atcggaccga tgcccgggat caggatgacg
      781 ccgaggccgg cgagcagacc gacggtgccg ccgatgccgg tgccgacgac cgcgcccgca
      841 cccgcgccgc tcttggcgcc ctcttgcatc gcgtgcgagc gatcgtcacc atgcgcgccg
      901 tcgctgtcgg ccgaggccgc gccggcggcg gtgccggact ggttggcagc gatgctgatg
      961 tcgttgcggt tgaagttgcg ggccaccagg gcctcgaccg cggcctgggc cgcggagaaa
     1021 tcgtcgaaca aaccgatgac ggtcttcatg gggtctctcc tcgtttgatg gttgggggtg
     1081 gaggtatcgt tggtgtgctc ggcggcgtga tcggtggagg tggcggggcc gaccgctcgg
     1141 tcgtgacctc ctccgaacgc agctcgatgt catgggtctc gacgcgctcg ctctgacgaa
     1201 cgcgcaggtg cagctcctcg cgcaacacga gtcgcttctc gaggacgagc acctcctcga
     1261 gcacagggta gatcgtcgtg tcgccctcgt ggcgcacggg cacgggtccg tcgaccacgc
     1321 ggttgatcgg gacccgcgtg atctcgatgt tctcgctgcg cagcggcacc tcgacgtgct
     1381 cggtgcggac gtggacgtgc ttgttgacac gcacggagcc ggcctcgcgg acctgcttgt
     1441 ggacctcgaa ggtctcctgc acgaccggca ccgtcgcaac cacggagctg acgctgacct
     1501 cggcgatgat cacgtcatcg ctgaccaccg ggccggtcga cccgctggta tgcgtccctc
     1561 gcggcggcgc cgggctggtc gccatcgtct cgggcgacag caggtcggcg atgctgcacg
     1621 acaggaagta gttgctgtcg tcgagctgcg tcagcaagct cgtcggcacg cgcacgttgc
     1681 cgccgtcgct gagctggatc agcgtatcga cgcgatcggg ggtccagccg tcggtgccga
     1741 cgtcgataaa gccgcggacg ccgtcgcgat cggtcaccag gaggatcgcg ggagtgttca
     1801 tacggcgtag tacttgtagt agacgaatga cgcgatcgcg aggctgatga gcgccagtgc
     1861 gatcaatgag atgggtcggg ccgccagcgc caaagccccc ggacgatcat agatcttcac
     1921 ggcgctccgt cgctcggcgt cgggagtaaa aggggctttc ggggtattca tgcgtatcct
     1981 gcacaatgcc cggacatcgt ccgggccgcg ggcttcgctg gagtagcacc cgagtcccgc
     2041 atggcaacga cgcgaggtga aacactggac atcccccgtg gctgccccgc aggggcatcc
     2101 acgcagaaac agccagcgca tcccgcatgt cgcgccgcgc gaaacgtgac tgtgttcggt
     2161 ttcggccaaa gctctcagcg cagcgataac acccgcaacc catcgtcgag ccctgttccg
     2221 acatcacacc cgcgaccaca gcgtcgagga tgatgatcga tccgagcatc gctgctcgcc
     2281 ccgacgatga gacagcgacg gcctgaggct cccggacgac accagggtcg aggccggcgg
     2341 cggcgagcac ggcgaggcca acgacggcct gtgagggcct gcggaggggt ctggtaactc
     2401 ggggcgttgc gcgataaaac gtcgtcgccg ccatgccctc gcccgccccg acgttctccg
     2461 cgcgcagcta cgccttcgcg ccctacgctc cttgccacaa ctggatcgct ggtcagtgga
     2521 cccccgccgc ctccggcgag accctgcccg tccacaaccc gcgccatggg caggtcctcg
     2581 gccacgtcgc cctctcgggc gcggccgatg tcgcccgcgc cgtcgcggcc gccaaggccg
     2641 cgttcccggg ctggcgcgac accccgctca aggagcgcgt tcaggtcctg ttccgcctca
     2701 aggccctgat ggagcgcgac atcgaggagc tgacctggct ggtctcgcac gagaacggca
     2761 aggtctacgc cgaggccaag ggcgacgtgc t
//
LOCUS       DASWNJ010001739         3956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_490236, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001739 DASWNJ010000000
VERSION     DASWNJ010001739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490236"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VGB85_28970"
     CDS             <1..882
                     /locus_tag="VGB85_28970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138167.1"
                     /translation="VLAISALLIGGVASGIGAGRAAEASRATNQLANTVRFAFNKARV
                     TGTYYRLLINLDEGAFSMQRGDDRMYLPATDRYGRIRVVDPAREKEREERDRRAEENY
                     NRSVQGRVLAAIQGPSAGAKPGATPIGAPPGAPQKAAPGAPQKAAPAAAPAAPGAPGT
                     TAPMTDQYTVQPKKVPRRKPPIFGAFDDDNSLSELKKPFKLPKTIKVVSVQTAEDPKP
                     VTKGEASIYFFPQGHTQLAHIQIEEIEHPENAFTIIIRPLTGRVEIVDGKSDLALADD
                     PTAIKDDLGRRQTRRSF"
     gene            882..1649
                     /locus_tag="VGB85_28975"
     CDS             882..1649
                     /locus_tag="VGB85_28975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY0138168.1"
                     /translation="MSTLVTRKRRARKAGSHGLTLLEVMVAMAILAVSMTSLMSSQMA
                     SIRATRYAQQVTTIAFLAEYQLIEVEYMMRKEGGWILQDKIYENTFAPQGWPDIKYKC
                     IVDFLEIPDYSKLRAAKDESDKASQGESSVYYKNAGDKAFNALGMVWPMIKQAIERSI
                     RKVKCTVFWKDGSLQNEYSIYTFWADPEKLKTLPGLGGEAKEEDEADAQGGKPGGGGA
                     GGAGKGGGAPTGGGSSTGGGSMTGTLRPPGGAAGGGR"
     gene            1652..2365
                     /locus_tag="VGB85_28980"
     CDS             1652..2365
                     /locus_tag="VGB85_28980"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY0138169.1"
                     /translation="MATLDKYSRRNRRRRGLTMIEILVAVAIMTLMTVSVWVSFRSTA
                     QSMRHAEQLQVRYAIIRNSLSRMAAEMSMAYLSFNRPPDEDRHFTLFDGRDQFTQDNV
                     TFSAFAHIRMRKDANESDQSVIQYFFDRDPVVPGRMHLFRRESRRLAGDTPEKMMELF
                     PAYIFCEDIKSFDVKYWDIKKNDWVDEWRTTKQDMHPDRLPERVKITLGIMDPTTEKE
                     VKYTVQTLLFMQEKLDFSK"
     gene            2370..3722
                     /locus_tag="VGB85_28985"
     CDS             2370..3722
                     /locus_tag="VGB85_28985"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138170.1"
                     /translation="MTDPTDIQPAAPARKRPRKKGSGREHGVAILMVLACLAVLMPFT
                     AAFSYTTRVDWQAAINLRDEVAARNLNRGGLRLSLLLFELQRMVFNQKQFRQMMGSWD
                     ITQVAPYLMSAFGSKEGHEGIAGMAGLDASTFKELAIDNGSFEVRMEAESGKLNINCL
                     GNTGQGKDSSQARTVETIEAMLMPTLYDALFEEEKGDGQRYTRAQVIQAMVDYIDDDN
                     KSYDVVKLAASSTQERYRYTELFDPYQPRNARLDSIDELHLAEGVDDDLMMAIGADLT
                     VYGSCKVNLNFASADQIALVIRHSVDAADKWKTEGENFLMKTMPLARYVVDQRVMALF
                     PDIKAFEELVEKPDKFLSPLAMLGGASSPGAMRNANMPKIPEGIQIRLNSEKKEGSED
                     EMLGGASDIATVAPERVYRVEVIAEAGAVKKRLTAIYDMQFARAQSKGSGAWVYYRED
                     "
     gene            3742..>3956
                     /locus_tag="VGB85_28990"
     CDS             3742..>3956
                     /locus_tag="VGB85_28990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138171.1"
                     /translation="MAQKYVGLDLGTHEIKAVLISAGLRTVQVLEVVVESVAARSGAD
                     DVGAALEAGLAILRRRGWNHYRVGVSLP"
BASE COUNT          755 a         1387 c         1284 g          530 t
ORIGIN      
        1 gtgctcgcca tcagcgccct cctgatcggc ggcgtcgcgt ccggcatcgg cgcgggccgg
       61 gccgcggagg ccagccgcgc gaccaaccag ctcgccaaca ccgtgcgctt cgccttcaac
      121 aaggcgcgcg tcaccggcac ctactaccgc ctgctgatca acctcgacga gggcgccttc
      181 tcgatgcagc gcggcgacga ccgcatgtac ctgcccgcga ccgaccgcta cggccgcatc
      241 cgcgtcgtcg accccgcccg cgagaaggag cgagaggagc gcgaccgccg ggccgaggag
      301 aactacaacc gctcggtgca gggccgcgtg ctcgcggcga tccagggccc ctccgccggc
      361 gccaagccgg gcgccacgcc gatcggcgcc ccgcccgggg ccccgcaaaa ggccgccccc
      421 ggagccccgc aaaaggccgc cccggccgcc gccccggcgg ctcccggcgc gcccggcacg
      481 accgccccga tgaccgatca gtacacggtg cagcccaaga aggtcccgcg gcgcaagccc
      541 ccgatcttcg gcgccttcga cgacgacaac agcctctccg agctcaagaa gccgttcaag
      601 ctgcccaaga cgatcaaggt cgtcagcgtc cagaccgccg aggatcccaa gccggtcacc
      661 aagggcgagg cgagcatcta cttcttcccg cagggccaca cgcagctggc ccacatccag
      721 atcgaagaga tcgagcaccc cgagaacgcg ttcacgatca tcatccgccc gctgaccggc
      781 cgcgtcgaga tcgtggacgg caagagcgac ctcgcgctcg ccgacgatcc gaccgcgatc
      841 aaggacgacc tcggccggcg tcagacccgg aggagcttct gatgtccacg ctcgtcacac
      901 gcaagcgccg cgctcgcaag gccggcagcc acgggctcac cctgctcgag gtgatggtgg
      961 cgatggcgat cctcgcggtg tcgatgacct cgctgatgtc gtcgcagatg gcgagcatcc
     1021 gcgcgacccg ctacgcccag caggtgacga cgatcgcctt cctcgcggag taccagctga
     1081 tcgaggtcga gtacatgatg cgcaaggagg gcggatggat cttgcaggac aagatctacg
     1141 agaacacctt cgcgccgcag ggctggccgg acatcaagta caagtgcatc gtcgacttcc
     1201 tcgagatccc cgactacagc aagctgcgcg cggccaagga cgagagcgac aaggccagcc
     1261 agggtgagag cagcgtctat tacaagaacg ccggcgacaa ggccttcaac gcgctcggca
     1321 tggtctggcc gatgatcaag caggcgatcg agcggtcgat ccgcaaggtc aagtgcaccg
     1381 tgttctggaa ggacggcagc ctgcaaaacg agtactcgat ctacacgttc tgggccgacc
     1441 ccgagaagct caagaccctc ccgggcctcg gcggcgaggc caaggaagag gacgaggccg
     1501 acgcgcaggg cggcaagccc ggcggcggcg gtgcgggtgg ggcaggcaag ggcggcgggg
     1561 ccccgaccgg cggcggcagc agcaccggcg gcggctcgat gaccggcacg ctgcgtccgc
     1621 cgggcggcgc ggccggcggg gggcgatgac catggcgacg ctcgacaaat acagccggcg
     1681 taaccgccgt cgtcgcggtt tgacgatgat cgagatcctc gtcgcggtcg cgatcatgac
     1741 gctgatgacc gtgtcggtgt gggtcagctt ccgctcgacc gcgcagagca tgcgccacgc
     1801 ggagcagctc caggtccgct acgcgatcat ccgcaactcg ctcagtcgca tggcggccga
     1861 gatgtcgatg gcgtacctct cgttcaaccg cccgcccgac gaggaccgcc acttcacgct
     1921 gttcgacggc cgcgaccagt tcacccagga caacgtgacc ttctccgcct tcgcgcacat
     1981 ccgcatgcgc aaggacgcca acgagagcga ccagtcggtc atccagtact tcttcgaccg
     2041 cgacccggtc gtgccgggcc gcatgcacct gtttcgccgc gagagccggc gcctcgccgg
     2101 cgacacgccg gagaagatga tggagctgtt cccggcctac atcttctgcg aggacatcaa
     2161 gtcgttcgac gtcaagtact gggacatcaa gaagaacgac tgggtcgacg agtggcggac
     2221 gaccaagcag gacatgcacc cggaccgcct cccggagcgc gtcaagatca ccctcggcat
     2281 catggatccg acgaccgaga aggaagtcaa gtacaccgtc cagaccctgc tgttcatgca
     2341 agagaagctc gacttctcga agtgatgcca tgaccgaccc caccgacatc cagcccgccg
     2401 cgcccgcccg caagcgcccg cgtaaaaagg gcagcggccg cgagcacggc gtcgccatcc
     2461 tgatggtgct cgcctgcctc gcggtgctga tgccgttcac ggccgcgttt agctacacga
     2521 cccgcgtcga ctggcaggcg gcgatcaacc tgcgcgacga ggtcgccgcg cgcaacctca
     2581 accgcggcgg cctgcgcctg tcgctgctgc tgttcgagct ccagcgcatg gtgttcaacc
     2641 agaagcagtt tcgtcagatg atgggcagct gggacatcac ccaggtcgcg ccctacctga
     2701 tgtccgcgtt cggctccaag gagggccacg agggcatcgc cgggatggcc ggcctcgacg
     2761 cctcgacctt caaggagctg gcgatcgaca acggcagctt cgaggtccgc atggaggccg
     2821 agagcggcaa gctcaacatc aactgcctcg gcaacaccgg gcagggcaag gacagctcgc
     2881 aggcccgcac cgtcgagacg atcgaggcga tgctgatgcc gaccctctac gacgcgctgt
     2941 tcgaggagga gaagggcgac ggccagcgct acacgcgggc gcaggtgatc caggcgatgg
     3001 tcgattacat cgacgacgac aacaagagct acgacgtcgt caagctcgcc gccagctcga
     3061 cgcaggagcg ttaccgttac accgagctgt tcgacccgta ccagccgcgc aacgcccggc
     3121 tcgacagcat cgacgagctg cacctcgccg agggcgtcga cgacgacctg atgatggcga
     3181 tcggcgccga cctgaccgtg tacggcagct gcaaggtcaa cctcaacttc gccagcgccg
     3241 accagatcgc gctcgtgatc cgacactcgg tcgacgccgc cgacaagtgg aagaccgagg
     3301 gcgagaactt cctgatgaag acgatgccgc tggcccgcta cgtcgtcgac cagcgcgtga
     3361 tggccctgtt ccccgacatc aaggccttcg aggagctcgt cgagaagccg gacaagttcc
     3421 tgagcccgct ggcgatgctc ggcggcgcga gcagccccgg cgcgatgcgc aacgctaaca
     3481 tgccgaagat ccccgagggc atccagatcc gcctcaacag cgagaagaag gagggcagcg
     3541 aggacgagat gctcggcggc gcctccgaca tcgccaccgt cgcccccgag cgcgtctatc
     3601 gcgtcgaggt catcgccgag gccggcgccg tcaagaagcg cctgacggcc atctacgaca
     3661 tgcagttcgc gcgcgcccag tccaagggca gcggcgcgtg ggtttactac cgtgaagatt
     3721 gaaggttagg agcacccgcc catggcccag aagtacgtcg gtctcgatct cgggacccat
     3781 gagataaagg cggtgctgat cagcgccggc ctgcgcacgg ttcaagtgct ggaggttgtc
     3841 gtcgagtcgg tggcggcccg cagcggcgcc gacgacgtcg gcgcggcgct cgaggccggc
     3901 ctggcgatcc tgcgccgccg cggctggaac cactaccgcg tcggcgtgtc gctgcc
//
LOCUS       DASWNJ010001740         4155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_30606, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001740 DASWNJ010000000
VERSION     DASWNJ010001740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30606"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VGB85_28995"
     CDS             <1..865
                     /locus_tag="VGB85_28995"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY0138172.1"
                     /translation="ADASVSEVLVLGARSIEVRRDGQRAHHPTHFSCERALAVALDRL
                     IGAAPTELHPAVDGVTAAGVSVHAIGRPLVHGGSVIILSRPQPGPRTLAELVAQAQIS
                     AAAAEALAAGVARGANVLVCGPSGLDTSAWVGAIASEVPTPYRVVAVHRGRIARHLSE
                     RAITIDGSHHMEAALRSGLRMRPDCIIVHEVGGGEAVELCASARRGGGSTIVSLVADS
                     PEGALARLQAMVSLGVPRDPAALRGYISGCFDMILALHDAPSGRVVAGTLAEIRHAHA
                     GEVVELYTHDE"
     gene            920..1672
                     /gene="tssJ"
                     /locus_tag="VGB85_29000"
     CDS             920..1672
                     /gene="tssJ"
                     /locus_tag="VGB85_29000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HEY0138173.1"
                     /translation="MTPCDGRSLARRALRRAGGPLLLLQLVTACRGKNSTPPCEEPPT
                     VQALVQTSDRANVGENGQSWPTKLVLYQLAGTSSLDQLDAEALKEQGEKLFGDEFLDK
                     RELTAFPTTNERGELVIKPKATHLLVVAEFRETLGSAWYVTYAVPKGLRDAQCKATAD
                     DEPPPMPCVYLAIEGSELTGGAFSPPGFSLDKFEARCSVIAPPAKKKKKKKRLEKPKA
                     PDLNKRPPKVPGEDLSAPQAPNKPAAPSRPWR"
     gene            1678..3039
                     /gene="tssK"
                     /locus_tag="VGB85_29005"
     CDS             1678..3039
                     /gene="tssK"
                     /locus_tag="VGB85_29005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HEY0138174.1"
                     /translation="MSARPVWSEGMLLCPQHMQQQDLYHEYNLSARLAALTPMHWGVV
                     SQAFDASAVKAGQLALMGFRGVMPDGTPIAFEATSPQRPASRPIAPSFPANLRSVEVF
                     LALPTVREGVANYAIGEAAATMQRYRTATRSVFDLTQARSERELQFSDPNLTLLFGTE
                     ARDDYSAIKIAEVVRDEGGGFRISDDYMPPCLALSAAPMLVSGLQDLLSLVVTRRRKL
                     AEERRSRDGVRVEFNAQDITRYLFLQVLSGMVPLLRHFCDTPSLPLWPVYMELSRFAG
                     ELMTFATEGDPADLPGFNHTDPQAALGKLLPEIRRLLGAVIQDAFVTIPLRARQDNSW
                     IGELRDDKLLRCTQYVLAIEAEGEQQQIANEVPELAKVASWKRIPALVKNNTLGVPLR
                     ATHRPPSEIPIRPRQVYFMIATDDPHWREILNERTIAIYLKPPYDPRKARLTLMGIPA
                     RDA"
     gene            3047..3715
                     /locus_tag="VGB85_29010"
     CDS             3047..3715
                     /locus_tag="VGB85_29010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HEY0138175.1"
                     /translation="MHRIHELTKRVFDHVFLLLQPQAIADVARLHRSAADAIEAVEPR
                     ARKAGFSAEHARLIKYGLVALLDEVAQATSGPVAEYWEQHLLQREYFNEVRAGEGFFT
                     QLERLLDAKRDDAGALDVLQIYATCIFLGFRGKFVARSDARGFDALMRKIDVRLKDRL
                     AGDEALPAVKPAEWREPSAPLRAPLWLACLAVLFSLVMLCGYDSELTDDAERLGERLG
                     GPRP"
     gene            3712..>4155
                     /locus_tag="VGB85_29015"
     CDS             3712..>4155
                     /locus_tag="VGB85_29015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138176.1"
                     /translation="MKMLVGLMAFLALGVMFSQIAVSRFGVSPEDARLLLYVITGVWM
                     LGMIIMLVRWIIARRQAAAKNAKPIDPAVLVLRKRTAEARAALDQLARQQKAVAPKRP
                     WLVTAPYATPWLAVLGLPQHGKTALLGPISGKRLVEVDQEGPRKGP"
BASE COUNT          614 a         1519 c         1442 g          580 t
ORIGIN      
        1 cgccgacgcc tccgtctccg aggtgctcgt gctcggcgct cgctcgatcg aggtccgccg
       61 cgacggccag cgggcgcatc acccgaccca cttctcgtgc gagcgcgcgc tcgcggtcgc
      121 gctcgaccgc ctgatcggcg cggccccgac cgagctgcac ccggccgtcg acggcgtcac
      181 cgcggccggc gtctccgtgc acgcgatcgg gcgcccgctg gtccacggcg gctcggtcat
      241 catcctctcg cgcccgcagc cgggcccgcg cacgctcgcg gagctggtcg cgcaggcgca
      301 gatcagcgcc gccgcagccg aggcgctcgc cgccggtgtg gcccgcggcg ccaacgtgct
      361 cgtgtgcggc ccgtccgggc tcgacaccag cgcctgggtc ggcgcgatcg cgtccgaggt
      421 gccgacgccc taccgcgtgg tcgcggtcca tcgcggtcgc atcgcccggc acctcagcga
      481 gcgcgcgatc acgatcgacg gatcgcacca catggaggcc gcgctgcgca gcgggctgcg
      541 gatgcgcccc gactgcatca tcgtgcacga ggtcggcggc ggcgaggcgg tcgagctgtg
      601 cgcctcggcc cgccgcggcg gcggctcgac gatcgtcagc ctggtcgcgg actcgcccga
      661 gggcgcactc gcgcggctgc aagcgatggt ctcgctcggt gtgccccgcg atccggccgc
      721 gctccgcggc tacatctcgg gctgcttcga catgatcctc gcgctgcacg acgcgccgag
      781 cgggcgcgtg gtcgcgggta cgctcgcgga gatcagacac gcgcatgccg gcgaggtcgt
      841 cgagctctac acgcacgacg aatgatccgc gcgcttgtga cacacgactg tcctcgcagg
      901 ttcgtggtac gttggccgcg tgacaccctg cgatggtaga tcgctcgcca ggcgcgcgct
      961 gaggcgtgca ggaggcccgc tcctgctgct ccagctcgtc acggcctgcc gcggcaaaaa
     1021 ctcgacaccg ccctgcgagg agccgccgac cgtacaggcg ctggtgcaga cctcggaccg
     1081 cgccaacgtc ggcgagaacg gccagtcgtg gccgaccaag ctggtccttt accagctggc
     1141 cggcacgtcg tcgctcgacc agctcgacgc cgaggcgctc aaggagcagg gcgagaagct
     1201 gttcggcgac gagttcctcg acaagcgcga gctgaccgcc ttcccgacca ccaacgagcg
     1261 cggcgagctg gtgatcaagc ccaaggcgac gcacctgctg gtcgtcgccg agttccgcga
     1321 gaccctcggc agcgcgtggt acgtgaccta cgccgtgccc aagggcctgc gcgacgcgca
     1381 gtgcaaggcg accgcggacg acgagccgcc gccgatgccg tgcgtgtacc tggcgatcga
     1441 gggcagcgag ctgaccggcg gcgccttctc gccgccgggc ttctcgctcg acaagttcga
     1501 ggcgcgctgc tcggtgatcg cgccgcccgc caagaagaag aaaaagaaga agcgcctcga
     1561 gaagccgaag gcgcccgatc tcaacaagcg gccgcccaag gttccgggcg aggacctgag
     1621 cgctccccag gcgccgaaca agcccgcggc tcccagccgc ccatggaggt gacccgcgtg
     1681 tctgcaagac cagtatggag cgaggggatg ttgctgtgcc cgcagcacat gcaacaacag
     1741 gacctctatc acgagtacaa cctgtcggcg cggctggccg cgctgacgcc gatgcactgg
     1801 ggcgtcgtct cccaggcgtt cgacgcgagc gcggtcaagg ccgggcagct ggcgctgatg
     1861 ggctttcgcg gggtgatgcc ggacggcacg ccgatcgcgt tcgaggcgac gagcccccag
     1921 cgaccggcga gccgaccgat cgcgccgtct ttcccggcca acctgcgcag cgtcgaggtg
     1981 ttcctcgcgc tgccgaccgt gcgcgagggc gtcgccaact acgcgatcgg cgaggccgcg
     2041 gcgacgatgc agcgctaccg caccgcgacc cgcagcgtgt tcgacctgac gcaggcccgc
     2101 agcgagcgcg agctgcaatt cagcgacccc aacctgacgc tgctgttcgg caccgaggcg
     2161 cgcgacgact actcggcgat caagatcgcc gaggtcgtgc gcgacgaggg cgggggcttt
     2221 cgcatctccg acgactacat gccgccgtgc ctggccctca gcgccgcgcc gatgctggtg
     2281 tcaggcctgc aggacctgct cagcctcgtc gtcacgcgcc ggcgcaagct cgccgaggag
     2341 cggcgctccc gcgacggcgt gcgcgtcgag ttcaacgccc aggacatcac ccgctacctg
     2401 ttcttgcagg tgctaagcgg catggtgccg ctgctgcgtc acttctgcga cacgccgagc
     2461 ctgccgcttt ggccggtgta catggagctg tcgcgcttcg cgggcgagct gatgaccttc
     2521 gcgaccgagg gcgacccggc cgacctgccg ggcttcaacc acaccgaccc gcaggcggcg
     2581 ctcggcaagc tgctgccgga gatccggcgg ctgctcggcg cggtgatcca ggacgccttc
     2641 gtcacgatcc cgctgcgcgc ccgccaggac aactcgtgga tcggcgagct gcgcgacgac
     2701 aagctgctgc gctgcacgca gtacgtgctc gcgatcgagg ccgagggcga gcagcagcag
     2761 atcgccaacg aggtgcccga gctcgccaag gtcgccagct ggaagcgcat cccggcgctc
     2821 gtcaagaaca acaccctcgg cgtgccgctg cgggccacgc atcgcccgcc gtcggagatc
     2881 ccgatccgtc cgcgccaggt ctacttcatg atcgccaccg acgacccgca ctggcgagag
     2941 atcctcaacg agcggacgat cgccatctac ctcaagccgc cctacgaccc gcgcaaggcc
     3001 cgtctgaccc tgatggggat ccccgcgcgc gacgcgtgag ccgcgaatgc accggatcca
     3061 cgaactgacc aagcgggtct tcgaccacgt gttcttgctg ctccagccgc aagcgatcgc
     3121 cgacgtcgcg cgcctgcacc gcagcgccgc cgacgcgatc gaggccgtcg agccgcgcgc
     3181 gcgcaaggcc ggcttctccg ccgagcacgc gcgcctgatc aagtacggcc tggtcgcgct
     3241 gctcgacgag gtcgcgcagg ccaccagcgg gccggtcgcc gagtactggg agcagcacct
     3301 gctgcaacgc gagtacttca acgaggtgcg cgcgggcgag ggcttcttca cccagctcga
     3361 gcgcctgctc gacgccaagc gcgacgacgc cggcgcgctc gatgtcctgc agatctacgc
     3421 gacctgcatc ttcctcgggt ttcgcggcaa gttcgtcgcg cgatcggacg cgcggggctt
     3481 cgacgcgctg atgcgcaaga tcgatgtccg gctcaaggat cggctcgcgg gtgacgaggc
     3541 gctgccggcg gtcaagccgg ccgagtggcg cgagccgagc gcgccgctgc gggccccgct
     3601 gtggctggct tgcctggccg tgttgttctc gctggtgatg ctgtgcggct acgacagcga
     3661 gctgactgac gacgccgagc gcctgggcga gcgtctggga gggccgcggc cgtgaagatg
     3721 ctcgtcggcc tgatggcctt cctggcgctc ggcgtcatgt tctcgcagat cgcggtctcg
     3781 cggttcggcg tatctcccga ggacgcgcgg ctcctcctct acgtgatcac cggcgtgtgg
     3841 atgctcggca tgatcatcat gctggtgcgg tggatcatcg cccggcggca ggcggcggcc
     3901 aagaacgcca agccgatcga cccggcggtc ctggtgctgc gcaagcggac cgccgaggcc
     3961 cgcgccgcgc tcgaccagct cgcgcgccag cagaaggccg tcgcgcccaa gcggccatgg
     4021 ctggtcaccg ccccgtacgc gacgccgtgg ctcgccgtgc tcggcctgcc gcagcacggc
     4081 aagacggcgc tgctcgggcc gatcagcggc aagcggctcg tcgaggtgga ccaggaaggc
     4141 ccgcgcaagg gcccg
//
LOCUS       DASWNJ010001741         3559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_170953, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001741 DASWNJ010000000
VERSION     DASWNJ010001741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170953"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VGB85_29020"
     CDS             complement(<1..209)
                     /locus_tag="VGB85_29020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY0138177.1"
                     /translation="MPLPTVEELGPRFADFVARLKRPLIFFDTETTGTDPANDRVVEI
                     SMIKISPGEGISPPRTWRINPNVRIP"
     gene            complement(332..1096)
                     /locus_tag="VGB85_29025"
     CDS             complement(332..1096)
                     /locus_tag="VGB85_29025"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY0138178.1"
                     /translation="MRTALAIAELQASDPALYDKLLKIKPNRWVGNELFFAQKEIADP
                     RAAPVLLKRLLNGEDSVRTRLAVVDALPNTGGDWYEGAAALIAIDADPRVRKKLVEVM
                     RYIPWPHNIDGLRLAFTDETASVRVAAARTTGFVRDGAGLYNELMNGTFDDDWDMRAA
                     CVQAIGQLRATAARDRLVGMLADDQAEVRLQVMLALDRIDPEGLRQLPELERLARERD
                     HWKVAAMARRLIQDAKAPMPAGAVATTAGASVSASP"
     gene            1602..1865
                     /locus_tag="VGB85_29030"
     CDS             1602..1865
                     /locus_tag="VGB85_29030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138179.1"
                     /translation="MNDAPTVRCDFLDVLEESVTIQKPVTVTLRDGKSFTDQVQDVVT
                     EEGEDFVNFASQGRVPVTQIERCEPSPQTHAPATYDDKLPDSE"
     gene            complement(1873..3426)
                     /locus_tag="VGB85_29035"
     CDS             complement(1873..3426)
                     /locus_tag="VGB85_29035"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEY0138180.1"
                     /translation="MTAPAANNDWKPNGKWILVAGTGDQARLEAPAQAVARALGTELA
                     EAGYGLVTGGWPGVDFHVAETFVARLRALGVDPGQHLKQVVHRDHQPSIREGTVVFVD
                     VGLPEYTQSVKLSDALVVIAGLGGTYQTHLLARELGRPVLPVASAEGDGRRAYNEIRR
                     SWDQQSAYSTITLPQFDQLGSPIDEPGRIVSVVLTLLGHLLAAVPAVRPPQTPSAAPT
                     ARSRARDPAAALGTDNEKMFQKLVETFRDPGVVGFIGAGCSIRAGYPSWPALLDKLEE
                     ALHDSPFKAQLDGVRSIPDFLWRAGRYRRELGTERYNQLIRELFGRKAGKPDEFHHDL
                     VALPFRHVITTNYDDTLDRAHVSVFHEPVDRVEWDNAHMVQQLIAELGSPTASRRYVY
                     LHGRFDNPESIILTEQDYQARYATGGADAKLSEMFGKRLTFIGFSLSDMDVMYMFRRL
                     KAQIGYEAPRHFALMALDTERDDSWAARAWLNEKYGVEPVFYPSSPDHAGLHVLVKRL
                     LAELGVSRP"
BASE COUNT          511 a         1151 c         1372 g          525 t
ORIGIN      
        1 gggatgcgga cgttcgggtt gatccgccac gtgcgcggcg ggctgatgcc ctccccgggg
       61 gagatcttga tcatcgagat ctcgaccacg cggtcgttcg ccgggtcggt tcccgtggtc
      121 tcggtgtcga agaagatcag cggacgcttg agcctggcga cgaagtcggc gaagcgcggg
      181 ccgagttcct cgacggtcgg gaggggcatg cgtgggctcg gcataagtcg gggtgggtta
      241 tacccggccc ggagcgctcg cggtacgcct gcgcgtgatc tggttgggag caggcgagcc
      301 cgcgggacag ctcacggcca cgagcgtgga ctcagggcga ggccgagacg gaggcgccgg
      361 cggtcgtcgc caccgcgccc gcgggcatcg gcgccttcgc gtcctggatc aggcgccgcg
      421 ccatggcggc gaccttccag tgatcccgct cgcgcgccag gcgctcgagc tccggcagct
      481 ggcgcaggcc ctcggggtcg atgcggtcga gcgcgagcat cacctgcagg cgcacctcgg
      541 cctggtcgtc cgccagcatc ccgacgagcc gatctcgcgc agcggtcgcg cgcagctggc
      601 cgatcgcctg cacgcaggcg gcgcgcatgt cccagtcgtc gtcgaaggtg ccgttcatca
      661 gctcgttgta caggccggcg ccgtcgcgca cgaagccggt ggtgcgcgcg gccgcgaccc
      721 ggaccgacgc ggtctcgtcg gtgaaggcga ggcgcaggcc gtcgatgttg tgcggccagg
      781 ggatgtagcg catgacctcg acgagcttct tgcgcacgcg cgggtcggcg tcgatggcga
      841 tcagggcggc ggcgccctcg taccagtcgc cgccggtgtt cggcagggcg tcgacgacgg
      901 cgaggcgggt gcgcacggag tcctcgccgt tgagcagccg cttgagcagc accggagccg
      961 cgcgtggatc ggcgatctcc ttctgggcga agaacagctc gttgccgacc cagcggttgg
     1021 gcttgatctt gagcagcttg tcgtagaggg cggggtcgct ggcctggagc tcggcgatcg
     1081 ccagggccgt gcgcacggtc caggcgaccg gcgccgcatc aggttgtctg gtgggctccg
     1141 gtgccggcgg ctcgacgagc gacagttcgt cggtcggcgg cggcgggggc atcggagcgg
     1201 gcgcaggcgc cggggaggtc ggcggttgca cgacctgagc acgcccggcg caagccggga
     1261 gcgcgaccat caacgacagg agcagcgcac acgaacgtga catcgaaggg atccttgttc
     1321 agcgtgcgag agactgcatc cgatgcaagg acgcggcgtg tcggagacca caagtgtgtg
     1381 cacttgtgta cccgcgtaac ccatcggcga tggcggtctg ggagcagctc gagcgggctc
     1441 cggcgtcctg cggtgatgtg ttcgtcggtg cgagcgtcgg ccgcgcaggg gcgcgccagg
     1501 gcccggtttg cgcgcgctca tcggtgcgtg gcggggccgc ggcgtgcccg ggcgggccac
     1561 cgcgcacacg ctccagcgac cccgtcacga ggacaaccac gatgaacgat gcacccaccg
     1621 tacgctgcga cttcctcgat gtgctcgagg agtcggtcac gatccagaag ccggtcacgg
     1681 tcacgctgcg cgacggcaag agcttcaccg atcaggtgca ggacgtcgtc accgaggagg
     1741 gcgaggattt tgtgaatttc gcgtcgcagg gccgcgtgcc ggtgacgcag atcgagcgct
     1801 gcgagccctc gccgcagacc cacgcgcccg cgacctacga cgacaagctg ccggactcgg
     1861 agtagtcgcc gtctacggcc gggacacgcc gagctcggcg agcaggcgct tgacgagcac
     1921 gtgcaggccg gcgtggtcgg gcgagctggg atagaagacc ggctcgaccc catacttctc
     1981 gttgagccag gcgcgcgcgg cccacgagtc gtcgcgctcg gtgtcgaggg ccatcagcgc
     2041 gaagtgccgc ggcgcctcgt agccgatctg cgccttgagg cggcggaaca tgtacatcac
     2101 gtccatgtcc gacagcgaga agccgatgaa cgtcaggcgc ttgccgaaca tctcgctgag
     2161 cttggcgtcg gcgcccccgg tggcgtagcg agcctggtag tcctgctcgg tgaggatgat
     2221 gctctcgggg ttgtcgaagc ggccgtggag gtagacatag cggcgcgacg cggtcgggct
     2281 gccgagctcg gcgatcagct gctggaccat atgcgcgttg tcccactcga cgcggtcgac
     2341 cggctcgtga aacacggaga cgtgcgcgcg gtcgagggtg tcgtcgtagt tcgtggtgat
     2401 cacgtggcga aacgggagcg cgacgaggtc gtggtggaac tcgtcgggct tgccggcctt
     2461 gcgcccgaac agctcgcgga tcaattgatt atagcgctcg gtgccgagct cgcggcgata
     2521 ccggcccgct cgccacagga agtcggggat cgaccgcacg ccgtcgagct gggccttgaa
     2581 gggcgagtcg tgcagcgcct cctcgagctt gtcgagcagc gccggccagc tcggataacc
     2641 ggcgcggatg ctgcaaccgg cgccgatgaa gccgacgacg cccgggtcgc ggaaggtctc
     2701 gacgagcttc tggaacatct tctcgttgtc ggtcccgagc gcggccgcgg ggtcgcgcgc
     2761 gcggctgcgg gcggtcggcg cggcggacgg ggtctgcggc gggcggacgg ccgggaccgc
     2821 ggcgagcagg tgcccgagca gggtgagcac gacggacacg atccgcccgg gctcgtcgat
     2881 cggggagccg agctggtcga actgcggcag cgtgatcgtc gagtaggcgg actgctggtc
     2941 ccagctgcgc cggatctcgt tgtatgcgcg gcgaccgtcg ccctcggcgg aggcgaccgg
     3001 gagcaccggg cggccgagct cgcgcgcgag caggtgagtc tgataagtgc cgccgagccc
     3061 ggcgatcacg acgagcgcgt ccgagagctt gacgctctgc gtgtactccg gcaggccgac
     3121 atcgacgaac acgacggtgc cctcgcggat gctcggctgg tggtcgcggt gcacgacctg
     3181 cttgaggtgc tgccccgggt cgaccccgag cgcgcgcagc cgggcgacaa aggtctcggc
     3241 gacgtggaag tcgacgcccg gccagccgcc ggtgacgagg ccgtaaccgg cctcagccaa
     3301 ctcggtcccg agggcgcgcg cgaccgcctg cgcaggcgcc tcgaggcgcg cctgatcgcc
     3361 cgtgccggcg acgaggatcc acttcccgtt gggcttccag tcgttgttcg ccgcgggggc
     3421 cgtcatcgct gccagtatac gacgagggcg gacgctcgcc gggggctcgc ggcgcggtcg
     3481 gtttggccgg gcgccgccgg ggcgacgctg gtcgggcgca ggacgatcgc cgacggcgca
     3541 cggtgtttga cgcggcggc
//
LOCUS       DASWNJ010001742         2812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_102346, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001742 DASWNJ010000000
VERSION     DASWNJ010001742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102346"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1397)
                     /locus_tag="VGB85_29040"
     CDS             complement(<1..1397)
                     /locus_tag="VGB85_29040"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: protein motif:HMM:NF014753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEY0138181.1"
                     /translation="MSHFINDRSSIVTEAIDAEVALSGGALRRLDGYPAIKVVVRGDW
                     DRGQVAVVSGGGAGHEPAHVGFVGDGMLTAAVCGEIFASPSVDAVLAGIVAVTGPPGC
                     LLIVKNYTGDRLNFGLAAEKARALGLAVEMVIVGDDIALRDAPRPRGVAGTLFVHKVA
                     GHLARAGASLAEVKAAAERVARATVSIGVSLSSCTIPGQPMENRIPEGQVELGLGIHG
                     EPGAQLLPLRPVREIVTTMMVLLEQAIGEKGPVAILVNDLGGVPPIELAIAGQAVIEA
                     ARHPVRLVFGPARLMTSLDMKGFSISMLVLRDREVEAALVSAVEPRAWPAARTPGPVT
                     PLALPDAIRPRARTASAHAGRRRVLACICEVLVARQAELDALDAKVGDGDTGTTLATA
                     ARTISADLDRLPFADAGELLAALSDALSRTMGGTSGILLAIFTAAVGARLGASEDWPA
                     ALAEGVRRVREYGGAT"
     gene            1530..2798
                     /locus_tag="VGB85_29045"
     CDS             1530..2798
                     /locus_tag="VGB85_29045"
                     /inference="COORDINATES: protein motif:HMM:NF015376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HEY0138182.1"
                     /translation="MSWRASAVLLCLACQPAGHGAGSPVQAGEWNLPEKTDSGGPVGS
                     SGVELPLDSTGGSTGERDEPASEPMPEPEPEPEVEEAEDDTDGETEDDTADDDPNRTW
                     TVHEVVPGETIAQLALRYRVDAGRIRGWNGLGRGASVRPGVRLRLLAKRTPPPRERVV
                     HTVREGDTWTSIARVHGCDPADLRAYNIKRTGRRLYPGLELEVWRDPLLADAFAADAP
                     PPGPAAAIRPGGFSVGRPSDGRLVNGVQIPESADYDRRYPGSAWGTTFAVRHLVDTLH
                     RWRAQSDYEGLLRLGAMSRQRGRRIGGHRSHQSGRDLDIRLPLRDGFADKVTPTPRRV
                     DWAAVHALVRAFDESGAVTRIFLDYRLQKRLYKVAKAAGADRKTLRRLIQFPRGPAAG
                     RGLVRHSPGHDIHIHVRFACAAHESECAGR"
BASE COUNT          405 a         1054 c         1002 g          351 t
ORIGIN      
        1 gtggccccgc cgtactcacg cacgcgacgc acgccctcgg cgagcgcggc gggccagtcc
       61 tcggacgcgc cgagccgggc accgaccgcc gccgtgaaga tcgccagcag gatcccgctc
      121 gtgccgccca tcgtgcgcga gagcgcgtcc gacagcgcgg ccagcagctc gccggcatcg
      181 gcgaagggca gacgatcgag gtcggcggag atcgttcgcg ccgcggtcgc cagggtcgtg
      241 ccggtgtcgc cgtcgccgac cttcgcgtcg agcgcgtcga gctcggcctg ccgcgcgacg
      301 agcacctcgc agatgcacgc gagcacccgg cgtcgtcccg cgtgcgccga ggccgtgcgc
      361 gcgcgcgggc ggatcgcgtc cggcagcgcg agcggcgtga cgggcccggg cgtgcgtgcg
      421 gccggccagg cgcgtggctc gaccgcggag acgagcgccg cctcgacctc cctgtcgcgc
      481 agcaccagca tcgaaattga aaagcccttc atgtcgagcg aggtcatcag ccgcgccggg
      541 ccgaagacga ggcggacggg gtgtcgcgcc gcctcgatca cggcctgtcc ggcgatcgcc
      601 agctcgatcg gcggcacgcc gccgaggtcg ttgacgagga tcgccaccgg ccccttctca
      661 ccgatcgcct gctcgagcag gaccatcatc gtcgtgacga tctcacgcac ggggcgaagc
      721 ggcagcagct gcgcgcccgg ctcgccgtgg atcccgaggc cgagctcgac ctgtccctcg
      781 gggatacggt tctccatggg ctggccgggg atcgtgcacg acgacagcga gacgccgatc
      841 gacacggtcg cccgcgccac gcgctccgcc gcggccttga cctcggcgag tgatgctccc
      901 gcgcgcgcga ggtggccggc gaccttgtgt acgaacaacg tcccggcgac gccgcgcgga
      961 cgcggggcgt cgcgtaacgc gatgtcgtcg ccgacgatca ccatctcgac cgcgaggccg
     1021 agcgcgcgcg ccttctcggc ggcgaggccg aagttgaggc ggtcgccggt gtagttcttg
     1081 acgatcaaca ggcaccccgg cgggcccgtg acggcgacga tccccgcgag caccgcgtcg
     1141 acgctcggcg acgcgaagat ctcgccacac accgcggcgg tcagcatgcc gtcaccgaca
     1201 aagccgacat gcgccggctc gtgccccgca ccgccgccgg agacgaccgc cacctgtccg
     1261 cgatcccagt cgccgcgcac gacgaccttg atcgccggat aaccgtcgag tcgccgcagc
     1321 gcaccgccgg acagcgcgac ctcggcgtcg atcgcctcgg tgacgatgct cgatcgatca
     1381 ttgatgaagt ggctcacgcg gcgcatcgta ccagcggtgg gcgggcacgg gcgacccgcc
     1441 cggtccgcga ccggcagaca gggcgggctc gacggccgca aatgatccga gattttgcgt
     1501 ggggcggccg gatcgtcgac agtgccggca tgtcctggcg ggcctcggcg gtgctcctct
     1561 gcctcgcctg tcagccggcg ggccacggcg cgggctcgcc tgtccaggcg ggcgaatgga
     1621 acctgcccga aaagacagat tctggggggc ccgtcggctc ctcgggcgtc gagctcccgc
     1681 tcgactccac gggcggatcg accggagagc gcgacgagcc cgcgagcgag ccgatgcccg
     1741 agcccgagcc cgagcccgag gtcgaggaag ccgaggacga caccgacggt gaaaccgagg
     1801 acgacaccgc cgacgacgac ccgaaccgga cctggaccgt gcacgaggtc gtgcccgggg
     1861 agacgatcgc ccagctggcc ctgcgctatc gcgtcgatgc cgggaggatc cgcggctgga
     1921 acggcctcgg gcggggggcc agcgttcgtc cgggcgtgcg cctgcgcctg ctcgccaaac
     1981 gcacgccgcc gccgcgcgag cgcgtcgtcc acacggtccg cgagggcgac acctggacgt
     2041 cgatcgcccg ggtccacggc tgtgatcccg ccgatctgcg cgcatacaac atcaagcgca
     2101 ccggtcggcg gctctacccc ggcctcgagc tcgaggtctg gcgcgaccct ttgctcgccg
     2161 atgcgttcgc cgccgacgcg ccgccgccgg gcccggccgc ggcgatccgt ccgggtggct
     2221 tcagcgtcgg ccggccgagc gacggtcgtc tcgtcaatgg cgtccagatc cccgagagcg
     2281 ccgactacga tcgacgctac cccgggtcgg cgtgggggac caccttcgcg gtccgccacc
     2341 tcgtcgatac cctgcaccgc tggcgcgcgc agagcgatta cgaggggctg ctgcggctcg
     2401 gcgcgatgag ccgccagcgt gggcggcgga tcggcggcca ccgctcgcac cagagcggcc
     2461 gcgacctcga cattcgcctg ccgctgcgcg acggcttcgc cgacaaggtg accccgacgc
     2521 cgcgccgcgt cgactgggcc gccgtgcacg cgctggtgcg ggccttcgac gagagcggcg
     2581 cggtcacccg gatcttcctc gattatcgcc tgcaaaagcg gctgtacaag gtcgcaaagg
     2641 cggcaggcgc cgatcgcaag accttgcggc gattgatcca gtttccccgg ggacccgcgg
     2701 ccggccgcgg cctcgtgcgc cactcgcctg gccacgatat ccacatccac gtccgcttcg
     2761 cctgcgccgc ccacgagagc gagtgcgccg ggcgctgagg atcgaccggg cc
//
LOCUS       DASWNJ010001743         2933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_626904, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001743 DASWNJ010000000
VERSION     DASWNJ010001743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626904"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1994)
                     /locus_tag="VGB85_29050"
     CDS             complement(<1..1994)
                     /locus_tag="VGB85_29050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138183.1"
                     /translation="MLRPALSLSCALTSAALLTACLNPNGTDLSGSDGSSTGTTSGDP
                     TDGVSSSGGPPTEPPPAAEWPTLDCDPLVPSYCGFPFPSNVFTRADETSKTGRRLALS
                     DALIPDGKKNMSGTPDVWNKADGFSAGLALMAHLPGATIEGLADPLTIERSLQADSPT
                     VVIDAETGERVPHFAELDVSGTDDTRRAFMIRPVVRLKDATRYIVAIRGVLGPDGTDR
                     PIEPSPAFKALRDIAPMADEPSVEARRPLYADIFERLDRAGVGRGDLQLAWDFTTATR
                     EHNTAGMLKMRDEALALVGPDGPEYTITKTTMDPTPDIGMVLEGMMTVPSYIDKPGPG
                     AHLVLGEDGLPQQNGMMDFKFTVVVPNSAFLTAGAPLQYGHGLLGSGGEEIQYEYRAK
                     FANTYNYVLFAVDWIGMAVDDYGHIGALVDQGRLNDFVTVVDRGQQGVLNALLAMRMV
                     SGRLAKDPLLQYKNGPMIKSDERYYHGNSQGGIFGSAYMALTTDVERGMLGVPGMPYS
                     LLLNRSKDFTPFFELLKNAFPDPIDLQLVLGLYQMLWDRTEPVGYTPYIRENTLPGTP
                     AHEVLMPVAIGDHQVSTLGAHIMARTIGGVVNIAPTNRSIFGIEEVPGPHVGSAMIEY
                     DFGLAPEPTTNIPPSDGEDPHGKVRKLGDAEKSLDQFLRTG"
     gene            complement(2083..>2933)
                     /locus_tag="VGB85_29055"
     CDS             complement(2083..>2933)
                     /locus_tag="VGB85_29055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138184.1"
                     /translation="TTAGISGETAASSTALAPRMSHVLGALALALLVSSAAPPEVHAS
                     GTFARQQLRYPRVKAAAAERLAAVEASFRDAGAVWPPAGVYLRGFKTEGVVELWAAGE
                     AGRAWVKVRDFEICASSGTLGPKRKQGDLQVPEGFYRVSRFNPRSAYHLALGVSYPNA
                     ADRFHSRGVAPGHAIMVHGNCVTVGCLPLEDGPIEDLYLAAMMARDAGQKTIPIHVFP
                     CRLDEDGCQATLATLATDRPDLAAFWAGLRPGYDAFVTTGAPPVVQARRDGSYLVVTP
                     RSRPGS"
BASE COUNT          420 a          933 c         1125 g          455 t
ORIGIN      
        1 cccgtgcgca ggaactgatc gagggacttc tcggcgtcgc cgagcttgcg gaccttgccg
       61 tgcgggtcct cgccgtcgga cggcgggatg ttggtcgtcg gctccggggc gaggccgaag
      121 tcgtactcga tcatcgcgga gccgacgtgc gggccgggga cctcctcgat gccgaagatg
      181 ctgcggttgg tcggcgcgat gttgacgacg ccgccgatcg tgcgcgccat gatatgtgcg
      241 ccgagcgtgc tgacctggtg atcgccgatc gccaccggca tcaggacctc gtgggcgggc
      301 gtgcccggca gcgtgttctc gcggatgtag ggcgtgtagc caaccggctc ggtgcggtcc
      361 cacagcatct gatacaggcc gagcaccagc tgcaggtcga tcgggtcggg gaaggcgttc
      421 ttgagcagct cgaagaaggg cgtgaagtcc ttgctgcggt tgagcaacaa actatacggc
      481 atgccgggca cgccgagcat gccgcgctcg acgtcggtgg tcagcgccat gtacgcgctg
      541 ccgaagatgc cgccttgact gttaccgtgg tagtagcgct cgtcggactt gatcatcggg
      601 ccgtttttgt attgcagcag cgggtccttg gcgagccggc cgctgaccat gcgcatcgcc
      661 agcagcgcgt tgagcacgcc ctgctggccg cgatcgacga cggtgacgaa gtcgttgagg
      721 cgaccctgat cgacgagggc cccgatatgt ccgtagtcgt ccacggccat gccgatccag
      781 tcgaccgcga acagcacata gttgtacgtg ttggcgaact tggcgcgata ctcgtactgg
      841 atctcctcgc cgccggagcc gagcaggccg tggccgtact gcagcggcgc gccggcggtc
      901 aagaaggcgc tattgggcac gacgacggtg aacttgaagt ccatcatgcc gttttgctgc
      961 ggcaggccgt cctcgccgag cacgaggtgc gcgccggggc cgggcttgtc gatgtagctc
     1021 ggcacggtca tcatgccctc gagcaccatg ccgatgtcgg gggtcgggtc catcgtcgtc
     1081 ttggtgatcg tgtattcggg gccgtcgggg ccgaccagcg cgagcgcctc gtcgcgcatc
     1141 ttgagcatgc cggcggtgtt gtgctcgcgc gtggcggtcg tgaagtccca ggcgagctgg
     1201 aggtccccac gaccgacgcc ggcgcggtcg aggcgctcga agatgtcggc gtagagcggc
     1261 cggcgggcct cgacggacgg ctcgtcggcc atcggcgcga tgtcccgcag ggccttgaac
     1321 gcgggcgagg gctcgatggg acggtcagtg ccgtcggggc cgaggacgcc gcggatcgcg
     1381 acgatgtagc gggtcgcgtc cttgagccgg acgaccgggc ggatcatgaa ggcgcggcgg
     1441 gtgtcgtcgg tgcccgacac gtcgagctcg gcgaagtgcg gcacgcgctc gccggtctcg
     1501 gcgtcgatga cgacggtcgg cgagtcggcc tgcagcgagc gctcgatcgt cagaggatcg
     1561 gccaggccct cgatcgtcgc gccgggcagg tgggccatca acgcgaggcc ggccgagaag
     1621 ccgtcggcct tgttccacac gtccggcgtg ccgctcatgt tcttcttgcc gtcggggatc
     1681 agcgcgtccg agagcgcgag gcggcggccg gtcttgctgg tctcgtcggc gcgcgtgaac
     1741 acgttcgagg ggaacgggaa gccgcagtag ctcggcacca gcgggtcgca gtcgagggtc
     1801 ggccactcgg cggccggcgg cggctcggtc ggcggacccc ccgagctgct gacgccgtcg
     1861 gtcggatcgc cgctcgtggt gccggtcgag ctgccgtcgc tgccgctgag gtcagtgccg
     1921 ttggggttga ggcaggcggt caggagcgcg gccgaggtca gcgcgcagga cagggacagg
     1981 gccgggcgga gcatcgccac gcgaggatat ccaagtgcgc gcggagcagc caccacgcat
     2041 cgccgacagc gtgagcgtgc aggctcgtgc gcaggctccg ttttagctgc cgggccgcga
     2101 tcgcggcgtg acgacgaggt agctgccgtc gcggcgggcc tggacgaccg gcggcgcgcc
     2161 ggtggtgacg aaggcgtcgt aaccggggcg caggccggcc cagaacgcgg cgaggtcggg
     2221 gcggtccgtg gcgagcgtgg cgagcgtcgc ctggcagccg tcctcatcga ggcgacacgg
     2281 aaatacgtgg atcgggatcg tcttctggcc ggcgtcgcgg gccatcatcg cggcgaggta
     2341 gaggtcctcg atcgggccgt cctcgagcgg cagacagccg acggtcacgc aattgccgtg
     2401 gaccatgatc gcgtggcccg gggcgacgcc gcggctgtgg aagcggtcgg ccgcgttggg
     2461 gtaactgacg ccgagcgcga ggtgatacgc gctgcgcggg ttgaagcggc tgacgcgata
     2521 aaaaccctcg gggacctgca ggtcgccctg cttgcgcttg gggccgagag tcccgctgct
     2581 ggcgcagatc tcgaagtcgc gcaccttcac ccaggcgcgc ccggcctcgc ccgcggccca
     2641 cagctcgacg acaccctcgg tcttgaagcc gcgcaggtac acgccggccg gcggccagac
     2701 tgcgcccgcg tcgcgaaagc tggcctcgac cgccgcgagg cgctcggcgg cggcggcctt
     2761 gacccgcggg tatcgcagct gctgccgggc gaacgtgcca ctggcgtgga cctccggggg
     2821 ggcggccgac gacaccagca acgcgagcgc gagggcgccg aggacatgcg acatgcgagg
     2881 tgccaacgcg gtcgacgacg cggcggtctc gccggatatt ccggcggtgg ttg
//
LOCUS       DASWNJ010001744         2671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_170985, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001744 DASWNJ010000000
VERSION     DASWNJ010001744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170985"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VGB85_29060"
     CDS             <1..673
                     /locus_tag="VGB85_29060"
                     /inference="COORDINATES: protein motif:HMM:NF025596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Druantia anti-phage system protein DruA"
                     /protein_id="HEY0138185.1"
                     /translation="RRRYASAESEIASSVAGRSIMRAPKLVLLGTTSLYGVGSSQYNR
                     IKIPVDRIGGANKDVIRFEELGRSEAFGTSHYSEETVDALVDLVQQSEEQRVNSIFGE
                     GASPKLRKVRQALDLLNLPTGLLLRHYRPRVVYGVSLIRNLEDYLIGLARKPDYIIPL
                     NEGPAATAQTGAWWRERWLRNRIASDEVLEEVARHTRVHPITHGARVVQQPASSQQPL
                     FAEPS"
     gene            670..1710
                     /locus_tag="VGB85_29065"
     CDS             670..1710
                     /locus_tag="VGB85_29065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138186.1"
                     /translation="MKTKSSARPRASQVTATKAVVRWYLEHHFRRPSDPGVIEMFCDS
                     ERVGAFAVDRNALRAGDSRALFRLLVATAMFQRRQDVQILRILQGMSAADAYEITDAE
                     RLVALVDESSCSHMRTTGALADECDLGKDPRTRLGCCSANPRVACHLKLHTVLLKRYG
                     HFGKVPTSIALMVRESGAEDLSALRRLVMKREREPLARAQELERELSRAWRVNQKIAS
                     MFLSMVTNPDLSRGLAPWTRGLDWTYYVVVDSNVDLFLASIGFSGGSSYDARRDFVRA
                     LSRGIDLTEFDETLRPYNPRLVQQAMYLFMSTANRRAAAADCMHLVPTSCATCPRAVR
                     SRCPVTRLNSAR"
     gene            1930..>2671
                     /locus_tag="VGB85_29070"
     CDS             1930..>2671
                     /locus_tag="VGB85_29070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138187.1"
                     /translation="MSRDLGVWNQRLAEHFGALARNRKDIPLFALEHGLAQAELLALQ
                     NDLRAQIQSSAPARDHQLVWTVYAAEIGYGYAGDQYWRSFEEMTPGWTVNGRRDWVRD
                     AFVAFQRRFAGAKPSGPWAEQFAIIAWPITHAILPRDLQQQLARILYDLRHAFSAELF
                     DEPQRLGDFIAARSGNTSARFKNLVQAPALVGQIAAALLLQGKDGFKTLLHPATLKRI
                     GDDVDRERRGRDWLRGARRVAEERAKIRG"
BASE COUNT          456 a          890 c          872 g          453 t
ORIGIN      
        1 ccgtcgtcgc tatgcgagcg cggagagtga gatcgcctcg tccgtcgcag ggcggtccat
       61 catgcgcgcg cccaagctcg tgctgctcgg tacgacgtcg ctctacggcg tcggatcgag
      121 ccagtacaac cgcatcaaga tccccgttga tcggatcggt ggcgccaata aggacgtcat
      181 ccggttcgag gaattgggac gctcagaggc attcggaacg tcccattact ccgaagagac
      241 cgtcgacgcg ctcgtcgacc tggtccagca gtccgaggag cagcgcgtga acagcatctt
      301 cggggagggg gcgagcccca agctccgtaa agtacggcag gcgctcgact tgctcaacct
      361 gcccaccggc ctgctcctcc ggcactaccg tccgcgcgtc gtctacgggg tgagcttgat
      421 ccggaacctc gaggattacc tcatcggcct cgcgcgaaag cccgactaca tcatccccct
      481 caacgagggc ccggccgcga cggctcagac cggcgcgtgg tggcgggagc ggtggcttcg
      541 aaaccggatc gcgtcggatg aggtcctcga ggaggtcgcg cgtcacacgc gggtccaccc
      601 gattacccac ggcgcgcgcg tggtgcagca gcccgcgtct agccaacagc cgctgttcgc
      661 cgagccgtca tgaagacaaa gagctccgcg cggccccgcg cgagccaggt tacggcgacc
      721 aaggcggtcg tgcggtggta cctggagcac catttccggc gcccctccga ccccggcgtc
      781 atcgagatgt tctgcgactc ggagcgcgtg ggcgcgttcg ccgttgatcg gaatgcattg
      841 cgcgccggtg acagtcgcgc cctctttcga ttgctcgtcg ccacggcgat gttccagcgt
      901 cggcaggacg tgcagatcct ccgcatattg caaggcatga gcgcggccga cgcgtacgaa
      961 ataaccgacg ctgagcgcct cgtcgcgttg gtcgacgaga gcagctgttc gcacatgcgc
     1021 accacgggag ctctcgccga cgagtgcgac ctcggcaagg acccgcggac ccgcctcggg
     1081 tgttgcagcg cgaacccgag ggtcgcgtgt catctcaagc tacacacggt gctgctcaag
     1141 cgctatgggc acttcggtaa ggtccccacg tcgatcgcct tgatggtcag ggagtcgggc
     1201 gccgaggatc tctcggcgct gcgtcgattg gtgatgaaga gagaaagaga gccgctcgca
     1261 cgtgcccagg agcttgagcg tgagctctca cgcgcatggc gcgttaacca gaagatcgcg
     1321 agcatgttcc tctcaatggt cacgaacccg gacctgtcgc gagggctcgc gccgtggacc
     1381 cgaggccttg actggaccta ctacgttgtc gtcgacagca acgtggacct cttcctcgcg
     1441 tccatcggat ttagcggagg cagcagctac gacgctcgcc gtgacttcgt ccgggcactc
     1501 tcgcgaggca tcgacttaac cgagtttgac gagacgctgc ggccgtacaa ccctcgcctc
     1561 gtgcagcagg cgatgtatct gttcatgagc accgcgaacc gccgggcggc agccgccgac
     1621 tgtatgcact tggtaccgac ctcgtgcgcg acatgccctc gcgcggtgcg gagccgctgt
     1681 ccggtgacac ggctgaacag cgcacggtag tcacacgccc cactttcccc gacacatccc
     1741 tgggcttcgt tcgctcgccc tttcgccact gtggcggggt ggggtacctt ggcttggtgc
     1801 catttcaccc cactgctgcg agcagcggag aagatcgcct tcaaggtcca cagacggtgg
     1861 ctcccttacg ccgcgctcgc ccctcgggga ccgcgtggta cgcgcctgca caagtaaggg
     1921 agtgagcaga tgtcgaggga tctgggggtg tggaaccagc gactggcaga gcatttcggc
     1981 gccctcgcgc gtaaccggaa ggacatcccc ctcttcgcct tggagcacgg cctcgcgcag
     2041 gctgagttgc tagcgctgca gaacgatctg cgcgcccaga tccagagttc ggcgcccgcc
     2101 cgcgaccatc agctcgtctg gaccgtctac gcagcggaga tcggctacgg ttacgcgggc
     2161 gaccagtact ggcggtcctt cgaggagatg acgccgggct ggactgtgaa cggcaggcgt
     2221 gactgggttc gtgacgcctt cgtcgccttc cagaggaggt tcgcgggcgc gaagccatcg
     2281 ggcccatggg cggagcagtt cgctatcatc gcgtggccca tcacgcacgc gattctcccg
     2341 cgcgacctgc aacagcaact cgcgcggatc ctctacgacc ttcggcacgc gttttccgcc
     2401 gaactcttcg acgagccgca gcgcctcggg gactttatcg cggcccggag cgggaacacg
     2461 tcggcgcgct tcaagaacct cgtgcaagcg cccgcgctcg tcggacaaat tgccgccgcg
     2521 ctgctcctgc aggggaagga cggtttcaag acgctcctcc acccggcgac gctcaagcgc
     2581 atcggcgatg acgtcgaccg cgagcgccgt ggtcgcgact ggctgcgcgg cgcacggcgc
     2641 gtagcggagg agcgggcgaa gatccgaggg c
//
LOCUS       DASWNJ010001745        11646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_667607, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001745 DASWNJ010000000
VERSION     DASWNJ010001745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 11646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11646
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667607"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="VGB85_29075"
     CDS             <1..797
                     /locus_tag="VGB85_29075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138188.1"
                     /translation="SMNASQGGPSGGGNGAGGFDNKRPGPAGSGMAGSSRPQGLASAP
                     ASGSRPGAGAPGNLSAGSGTRRVAGASAPTLAASPAGHSGGQGRGYTAPGTTGAFSAG
                     GGARSPGGPAAQASYRPTYAPSAMAYPSSGGGGSRGSLSAGGGSSYRPSSYTAGGGSR
                     GSMSSSSGRGGYVPSDSSSSFRGNGGSYSGGHSGSSGSSFGGGRSSGGFGGGGFGGGG
                     GGGSRGGGFSGGGGGGGGGFSGGGGGGGGGGRGGGGGFGGGGGRGR"
     gene            985..1734
                     /gene="gloB"
                     /locus_tag="VGB85_29080"
     CDS             985..1734
                     /gene="gloB"
                     /locus_tag="VGB85_29080"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEY0138189.1"
                     /translation="MPTLSIEPIPCLRDNYAYLVSDEDTGDAWLVDPSEPGPPGAVLR
                     RRGLRLRGILATHHHMDHVGGIATLLDQHRNVWVAGHTRDSGRIPRQTILVDAPADKF
                     ITTDLQMGGHGVQAMYIPGHTIGAIAWYLPPRTGSPGDVFTGDTLFAAGCGRLFEGSP
                     TQMYTSLQRLVALPPETRLWFGHEYTSANLRFAAAVEPDNTEVTGRAAKLPICTTPTT
                     VALELATNPFVRAGSAEVLAERRQAKDEFRG"
     gene            1864..2772
                     /locus_tag="VGB85_29085"
     CDS             1864..2772
                     /locus_tag="VGB85_29085"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEY0138190.1"
                     /translation="MFDLAAWVQAARPLAQINIVVPLLFGQALAFAALGRFGPGFMLL
                     AGLFGLLVQLVIVFVNDLSDQETDLHNVTYARVSGGSRVLAQGKLSPADLRGGAIVAL
                     GLLAVFSVGLALGGRPWMPVFAAAAVSLVWAYDRAPLRLAYRGRGELVQGLGTGVVLP
                     LAGYYIQAGTLAGFHPWALIPLLLLGYIGNLLSALPDAPSDRVAGKRTYAVRQGQWAT
                     RLTAFALTAIASTMSALAVPGLPLWAMVLLAAPVLAVVALAGPLVSDADAEKRAECER
                     FVMTLAGAGHLLVLLWSAVLVLPGLP"
     gene            complement(2793..3074)
                     /locus_tag="VGB85_29090"
     CDS             complement(2793..3074)
                     /locus_tag="VGB85_29090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138191.1"
                     /translation="MPYVEYACEGRLLFRTETTEDVQFVPRIHEVVVIDDAPYQVVDV
                     EYWARRLGSSDRRLVCPTIYVIPVGAAEWEQRLERRTPRARDGKPPVRY"
     gene            complement(3077..5167)
                     /locus_tag="VGB85_29095"
     CDS             complement(3077..5167)
                     /locus_tag="VGB85_29095"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY0138192.1"
                     /translation="MNRLSETTSPYLRQHADNPVDWYPWGEEALARARAEGKPILLSV
                     GYAACHWCHVMAHESFEDPEVAALMNERFINIKVDREERPDIDAIYQKVVQLMGQGGG
                     WPLTVFLTPDQRPFYGGTYFPPTPNYGRHSFRQVLLALGDLWQHRRAELDEQVKTFAE
                     GMADMARLADEDSRQVSVQTTLSDVHTLDEVVRRLLPKIDREWGGFGRQPKFPSPTNL
                     ELLLQATRSSAIDVAQKAGEALLLTLGKMWRGGIYDHLRGGFARYSTDRVWLVPHFEK
                     MLYDNAQLLGVYAEASRVWTDQPFLREVVRETATYLAADMRDPGGGFYAATDADSEGV
                     EGKFFCWTPAQLREQLGDDAAASRFAQVYGVTESGNFEHGMSILHLPRSFAEQAEALG
                     TSEEALRAELQPLRERLLAHRATRVPPLRDEKILTSWNGLAISGLVRAAEAAESWGEP
                     ELAREWLELATAAGQYLLANHVTVEGRVLRASFAGQAHTRGYAEDLGALARACLDLHE
                     ATLAPHWLQRAHDLAHHALIHHASPGGLGFYMTADDGEQLIERSECEHDSALPSGLGL
                     LVEVLLRLDASDAGPPGARATAEAVMHRYRGAVAQPFAYASLLAAARHGSALAVHVKL
                     LAETPEAATDRARRVRAERALLPGPLSLHFAEAAGPPTAIVCRAQACSAPLRDPAALA
                     PELHGAESFRYTDR"
     gene            5380..5464
                     /locus_tag="VGB85_29100"
     tRNA            5380..5464
                     /locus_tag="VGB85_29100"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5414..5416,aa:Ser,seq:cga)
     gene            5551..5635
                     /locus_tag="VGB85_29105"
     tRNA            5551..5635
                     /locus_tag="VGB85_29105"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5585..5587,aa:Ser,seq:gga)
     gene            5670..5765
                     /gene="ffs"
                     /locus_tag="VGB85_29110"
     ncRNA           5670..5765
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VGB85_29110"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5862..7040)
                     /locus_tag="VGB85_29115"
     CDS             complement(5862..7040)
                     /locus_tag="VGB85_29115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138193.1"
                     /translation="MRPGFLLASSLALLPSLAQASPYAHLHALQDPAGAASPGTTTTT
                     TTTTTVAPAPTPATDPSLRPTSSKAPPSKDLVDQPAPEPQPVVTPPAPPPEESLAPPP
                     PPPNTWTRHGFGIRGGLTVIPTWAVRGYVQSLTNALCRGNSIPSGNLGKGLTRVDGCN
                     FYIGGEYIYRQSENFDIVPAVAYHRVKAPDGLWLDDSEKDANGNPNLGAADYTQVNFS
                     HVSLQVDFIGRGNLIKTPDFAWQLGGGGGIGLGILTGKGFLQTPLGNPPGSPGGATCT
                     SLEDYKDFTRCTPHYQDEEANGKNATADTHGSLALDTTDPMSNPGRFAKCNKDSCLGS
                     DLDALGRKKGINLPVIPIINILVTTRFMIKDTFGINITGGWQTGFYFGGSLQYFFGNK
                     "
     gene            complement(7174..8595)
                     /locus_tag="VGB85_29120"
     CDS             complement(7174..8595)
                     /locus_tag="VGB85_29120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY0138194.1"
                     /translation="MQLTDLGDDPPRLLKLGLELPLVRRVWALGSWVMLAMITQFLVN
                     AADNFMVGRLDDHALATASQAALGLGMPFFWAVGGFFCAVAFGTQAMTARRYAEGDDA
                     RAGQVLWNSLVVATIAGLVGGLIGWFTTPTAIDFLAKASPEQAELGISYARMRMLGIP
                     AMVLTFSYKAFFDGIGRTRVHLYAALVMNLLNIGLNYLLIYGAPSLGIPAMALTGAGL
                     ASAVSTYVGLLIMFGISLGPASRRYQFYRWAHTRWSIVRDIVRLMLPSGSATVILMIG
                     FMLFMRFVGEIDARVGGNTYSAATKALMDTASMCFMPVIALGTATATAVSQSLGAGKP
                     NLAARYGWESVRLGVYAMLVVAAIFLAIPEQILRIWAPNDPAVAAAGAEALRYVAAGL
                     PALVVGLVLSQALYGAGANTFVMVVEGILHFGVLVPLSWLLGPRLGYGLDGVWLAAAL
                     YTNLMGLAMALKFLGRGWRTIRL"
     gene            complement(8643..9395)
                     /locus_tag="VGB85_29125"
     CDS             complement(8643..9395)
                     /locus_tag="VGB85_29125"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEY0138195.1"
                     /translation="MNTKIFAALATLTLACKPEPATQPPAEPTPAPVAPEVTGPPTSE
                     PPNTMSGEPMPAEPGTATTVATTAGSTEPAPAIDAGPAPTPASNKEPLPAALSTKIDA
                     RCGNDPGVGTAAKPFNLKTPEGKDISLASHRGKVVLLNFWGTWCKPCLKELPEFDRLY
                     RRYRKNGLVVIAIATDTEPEKVQEFAAERKLATKLALGGQQLADDYESSNFPFSFVID
                     GKGQIRGSYRGYKPECAGKLEQDIRTQLEQLK"
     gene            complement(9392..10627)
                     /locus_tag="VGB85_29130"
     CDS             complement(9392..10627)
                     /locus_tag="VGB85_29130"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0138196.1"
                     /translation="MQDLRWPTYALRDVTLDRAEPDEHGALRVIDSEGRSYLDAVNGV
                     GCAPLGHNHPRWVADVHAQLQRLTSAANSFTTAPQQVLAHKLAERFPIRDARVFLGSS
                     GTEATEAAIKLAVRSTGRSIIVAFERAFHGRSLGALSLTANTAYRHPYVRCLDEAAPE
                     RPFATAQVLRLPWDDLDAVRAAFAKHGKDIAAVFVEPVQGEAGVYPGSREFLVGLHGL
                     CREHGALLGADEIQCGGGRVGAWSAWTHIVGDDPALQPDILWLAKALGGGMPIGACLT
                     TATLAQAMGKGTHGTTFGGNPLACAAALATIEIIESEGLLATAAAQLPTLQRLAADDP
                     SARVHEVRGLGAMIGIQVGKPGEQIGTAVGDRLMRDHAILVTVCGGHTIRLLLPYRAG
                     EAELREVWRAVSASVEVST"
     gene            complement(10688..>11646)
                     /locus_tag="VGB85_29135"
     CDS             complement(10688..>11646)
                     /locus_tag="VGB85_29135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138197.1"
                     /translation="VALATPAHAAQAPDPPATADASDPPPSGQDEAIRQFRAARELYS
                     AGAYAAAARGFEASYAAAASPEAAYNAALAHDKVGDDLATMRWYRRYLATASPEDPSY
                     PQAQQRALELRARLGELRLQVDSTDEIREIRVNGEAVTLSDFPKLVPPGHVELRLIGA
                     GPGETADIPAEVAPGGTWTVQFTGFARPEAPEPVVPRPTTRVVRPDPTPVPPAPRVRP
                     LTALFWTGTGLTTASAIAMGVLGGLVLRQRAVHEDCGRTGTCTDTAGSFRRYRDATNV
                     MVGVTAGLAVITLALGIAALRDRRKAGRERVRITLSGLQLAF"
BASE COUNT         1605 a         3831 c         4486 g         1724 t
ORIGIN      
        1 actcgatgaa cgcgtcgcag ggcggaccca gcggcggcgg caacggtgcc ggcggcttcg
       61 acaacaagcg ccccggaccc gcgggctcgg gcatggccgg ctcgagccgc ccgcagggcc
      121 tcgccagcgc gccggccagc ggcagccggc ccggcgccgg cgcgcccggt aacctgtccg
      181 cagggtcagg tacgcggcgc gtcgcaggcg cgagcgcccc gaccctcgcg gccagccccg
      241 cgggtcactc cggcggccag ggtcgcggtt acaccgcccc gggcacgacg ggggccttca
      301 gcgccggtgg cggcgcgcgc tcgccgggcg gcccggcggc gcaggcctcg tatcgtccga
      361 cctacgcgcc ctcggcgatg gcgtatccca gcagcggcgg cggcggttcc cgcggcagcc
      421 tgagcgccgg cggcggctcg agctaccgcc cgagcagcta caccgcgggc ggaggctctc
      481 gcggctcgat gtcctcgtcg tccggtcgcg gcggctacgt gccctcggac agcagctcga
      541 gcttccgcgg caacggcggc agctacagcg gcggccacag cgggtcgagc ggcagcagct
      601 tcggcggcgg ccgcagcagc ggtggctttg gcggcggtgg cttcggcggc ggcggcggtg
      661 gtgggagccg cggcggtggc ttcagcggtg gtggcggcgg cggaggcggt ggcttcagcg
      721 gcggtggcgg tggcggtggc ggtggtggtc gcggcggcgg cggtggcttt ggcggcggtg
      781 gcggtcgcgg gcgctgagcg aacggccagc cggctgtcct ctcggacagc tgtgaaaagc
      841 aacatgtgat cgagcgactc gacctggcct cgcgccgggt cgagtcgtcg cgcgtggtac
      901 cgagatcacc gggcagaccc ggaagatgca ttggggtacg tcgcccctgc cctgcgcacc
      961 tgcgcgcggg tatgctccag gtccatgccg acgctgagca tcgagccgat cccctgcctg
     1021 cgcgacaact acgcctatct cgtcagcgac gaggacaccg gcgacgcctg gctggtcgac
     1081 ccctcggagc cgggcccgcc gggcgccgtg ctgcgccgtc gcggtctgcg tctgcgaggg
     1141 atcctcgcca cgcatcacca tatggaccac gtcggcggca tagcgaccct gctcgatcag
     1201 caccgcaacg tctgggtcgc cggccacacc cgcgacagcg gtcgcatccc ccgccagacg
     1261 atcctcgtcg acgcgcccgc ggacaagttc atcaccaccg acctgcagat gggcggccac
     1321 ggcgtccagg cgatgtacat ccccgggcac acgatcggcg cgatcgcctg gtacctgccc
     1381 ccgaggacag gttccccggg cgacgtgttc accggcgaca ccctgttcgc ggccggctgc
     1441 ggccgcctgt tcgagggctc accgacccag atgtacacga gcctgcagcg cctggtcgcc
     1501 ctcccgcccg agacccgcct gtggttcggc cacgagtaca cctcggccaa cctgcgcttc
     1561 gcggccgcgg tcgagccgga caacacggag gtcaccggac gcgcggcgaa gctgccgatc
     1621 tgcacgacgc cgacgaccgt ggcgctcgag ctggccacca acccgttcgt gcgcgcaggc
     1681 tcggccgagg tgctcgcgga gcggcggcag gcgaaggatg aatttcgcgg atgaagaggt
     1741 gaagaggtcg agaggtcgag aggcgcgaac ggcgagaggt gcgaacggcg agcaccgtga
     1801 gcgaccatgg cccggggtcg ccgcctctcg acgaaatggc cccgcctgat ctagcctcgg
     1861 ggcatgttcg acctcgccgc gtgggtccag gccgcgcgcc cgctggcgca gatcaacatc
     1921 gtcgtgccgc tgctgttcgg gcaggcgctg gccttcgccg cgctcgggcg cttcggcccc
     1981 ggcttcatgc tgctggcggg cttgttcggg ctgctggtgc agctcgtcat cgtgttcgtc
     2041 aacgacctgt cggaccagga gaccgacctg cataacgtca cctacgcgcg cgtgtccggc
     2101 ggctcgcgcg tgctggcgca gggcaagctg tcgccggcgg atctgcgcgg cggagccatc
     2161 gtggcgctgg ggctgctcgc cgtgttcagc gtcgggctcg cgctcggcgg acggccgtgg
     2221 atgccggtgt tcgcggccgc ggcggtctcg ctggtgtggg cctacgatcg ggcgccgctg
     2281 cgcctggcat atcgcgggcg cggcgagctc gtgcaggggc tcgggaccgg cgtggtcttg
     2341 ccgctggccg gctactacat ccaggcgggc acgctcgcgg ggtttcaccc gtgggccctg
     2401 atcccgctgc tgctgctcgg ctacatcggg aacttgctgt cggccctgcc cgacgcgccc
     2461 tcggaccgcg tggccggcaa gcgcacgtac gccgtgcggc agggccagtg ggcgacgcgg
     2521 ctgaccgcgt tcgcgctgac ggcgatcgcc tcgacgatga gcgcgctcgc cgtgccgggg
     2581 ctgccgctgt gggcgatggt cctgctggca gcgccggtgc tcgccgtcgt ggcgctggcg
     2641 ggcccgctgg tcagcgacgc ggacgccgag aagcgggccg agtgcgagcg cttcgtgatg
     2701 accctcgcgg gcgcagggca tctgctggtg ctgctgtggt ccgccgtgct ggtgctgccc
     2761 gggctcccgt gaacctgccc gaagggacag ttttaataac ggaccggcgg cttgccgtcg
     2821 cgcgcgcgtg gggtccgacg ctcgaggcgt tgctcccact cggcggcgcc gacggggatc
     2881 acgtagatcg tcggacacac gaggcggcgg tcgctcgagc cgaggcgccg ggcccagtac
     2941 tcgacgtcga cgacctggta cggggcgtcg tcgatgacca cgacctcgtg gatccgcggg
     3001 acgaactgga cgtcctcggt ggtctcggtg cgaaacagca gccggccttc gcaggcgtac
     3061 tcgacgtaag gcatggtcag cgatcagtgt agcgaaagct ctcggcaccg tgcagctccg
     3121 gcgcgagggc ggccgggtcg cgcagcgggg cgctgcaggc ctgcgcgcgg cacacgatcg
     3181 ccgtcggcgg accggcggcc tcggcaaaat gcaggctcag cggccccggg agcagcgccc
     3241 gctcggcccg cacccggcgc gcgcgatcgg tcgcggcctc gggggtctcg gcgagcagct
     3301 tgacgtgcac cgcgagcgcc gagccatgac gcgccgccgc cagcaggctg gcatacgcga
     3361 acggctgagc gaccgcgccg cggtaccggt gcatcaccgc ctcggccgtc gcgcgcgcgc
     3421 caggcgggcc ggcgtcgctg gcatcgaggc gcagcagcac ctcgacgagc agaccgaggc
     3481 cgctcggcag cgccgagtcg tgctcgcact cgctgcgctc gatcagctgc tcgccgtcgt
     3541 cggcggtcat gtagaagccg aggccacccg gcgaagcgtg gtggatcagc gcgtgatggg
     3601 cgaggtcgtg cgcgcgctgt aaccagtgcg gcgcgagggt cgcctcgtgg aggtcgaggc
     3661 acgcgcgcgc gagggcgccg aggtcctcgg cgtagccgcg ggtgtgcgcc tggccggcga
     3721 acgaggcccg gagcacgcgg ccctcgacgg tgacgtggtt ggcgagcagg tactgcccgg
     3781 cggccgtcgc cagctcgagc cattcgcggg ccagctccgg ctcgccccag ctctcggcgg
     3841 cctcggccgc gcgcacgagg ccggagatcg cgaggccgtt ccacgaggtc aggatcttct
     3901 cgtcgcgcag cggcgggacg cgggtggcgc gatgggcgag caggcgttcg cgcagtggtt
     3961 gcagctcggc ccgcagcgct tcttcgctgg tgccgagcgc ctcggcctgc tcggcgaagc
     4021 tgcgcggcag gtggaggatc gacatgccgt gctcgaagtt gccggactcg gtcactccgt
     4081 agacctgggc gaagcggctg gcggccgcgt cgtcgccgag ctgctcgcgc agctgcgccg
     4141 gggtccagca gaagaacttg ccctcgacgc cctcgctgtc ggcgtcggtc gccgcataaa
     4201 agccgccgcc ggggtcgcgc atgtcggcgg cgaggtaggt cgcggtctcg cggacgacct
     4261 cgcgcaagaa tggttgatcg gtccacacgc ggctggcctc ggcgtagacg ccgagcagct
     4321 gggcgttgtc gtacagcatc ttttcgaagt gcggcacgag ccacacgcgg tcggtgctgt
     4381 agcgggcgaa gccgccgcgc aggtggtcgt agatgccacc gcgccacatt ttgccgagcg
     4441 tcagcagcag cgcctcgccg gctttctggg cgacgtcgat cgcggaggag cgggtcgctt
     4501 gcagcagcag ctcgaggttg gtcgggctcg ggaatttggg ttgccggccg aagccgcccc
     4561 actcgcggtc gatcttcggc agcaggcggc gcacgacctc gtcgagggtg tggacgtcgg
     4621 acagggtggt ctggaccgag acctggcggc tgtcctcgtc ggcgaggcgg gccatgtcgg
     4681 ccataccctc ggcgaaggtt ttgacctgct cgtcgagctc ggcgcggcgg tgctgccaga
     4741 ggtcgccgag cgccagcaag acctggcgaa acgagtggcg gccgtagttg ggcgtcggtg
     4801 ggaagtaggt cccgccgtag aacgggcgct ggtcgggggt gaggaacacg gtcagcggcc
     4861 agccgccgcc ctgccccatc agctgcacga ccttttggta gatcgcgtcg atgtcggggc
     4921 gctcctcgcg gtcgaccttg atgttgatga accgctcgtt catcagcgcc gcgacctccg
     4981 ggtcctcgaa ggactcgtgg gccatgacgt ggcaccagtg acaggcggcg tagccgaccg
     5041 agagcaggat cggcttgccc tcggcgcgcg cgcgggcgag ggcctcctcg ccccacgggt
     5101 accagtcgac cgggttgtcg gcgtgctggc gaaggtaagg gctcgtggtc tcgctcagtc
     5161 ggttcacggg cttcctccag gacgggcgcg cagcggacaa tagaggcaac gggcgcctca
     5221 ggctcgttat ggccggttcc gggccggctt gcccggcggt cccccggcga ttccaccggt
     5281 ccggagcgtg agtcttcgct caggcggctg gtgcggtcca gaggagattt ctggtatgtt
     5341 ccgcgccccg ctcggtcggg ccgaaaggct cgaccctctg gagaggtgtc cgagtggtcg
     5401 aaggtgcctg actcgaaatc aggtgtggcg aaagtcaccg ggggttcgaa tcccttcctc
     5461 tccgctacag cttcctgcca actgcatagc cgcgattctt tgagaacttc ttcctcttaa
     5521 aaagaagaag aagaaacagc cccgaccaag ggagaggtgt ccgagtggtc gaaggtgcat
     5581 gattggaaat catgtgtggc gaaagtcacc gagggttcga atccctccct ctccgctctg
     5641 atgctttgtt cgcaagagca agacatcgga actatgcccc gagcgacggc tctctcgcca
     5701 accccgccag gaccggaagg tagcaacggt aacgagagaa cgggtgctcg gggcacctat
     5761 attttctggt cgaggaagat cgcggacatg tctcggcgga catgtgcaaa gggacagagc
     5821 ccggtgggcc ctgtcccttt tgtcgttcgc gggcgcgcgg cttacttgtt accgaagaag
     5881 tactggaggc tgccgccgaa gtagaagccg gtctgccacc cgccggtgat gttgatgccg
     5941 aaggtgtcct tgatcatgaa gcgggtcgtc accaggatgt tgatgatcgg gatgaccggc
     6001 aggttgatgc ccttcttgcg gccgagcgcg tcgaggtcgg agccgaggca gctgtccttg
     6061 ttgcacttgg cgaagcggcc ggggttgctc atcgggtcgg tggtgtcgag cgcgagcgag
     6121 ccgtgggtgt cggcggtcgc gttcttgccg ttggcttcct cgtcctggta gtggggcgtg
     6181 cagcgggtga agtccttgta gtcctcgagc gaggtgcagg tcgccccgcc ggggctgccg
     6241 ggcgggttgc cgaggggcgt ctgcaggaag cccttgccgg tgaggatgcc gaggccgata
     6301 ccgccgccgc cgccgagctg ccaggcgaag tcgggggtct tgatcaggtt gccgcggccg
     6361 atgaagtcga cctgcagcga cacgtggctg aagttgacct gcgtgtagtc ggcggcgccg
     6421 aggttggggt tgccgttggc gtccttctcg ctgtcgtcga gccacaggcc gtccggggcc
     6481 ttgacgcggt ggtaggcgac cgcgggcacg atgtcgaagt tctccgactg gcggtagatg
     6541 tactcgccgc cgatgtagaa gttgcagcca tcgacgcggg tcaggccctt gccgaggtta
     6601 cccgagggga tcgaattgcc gcggcacagc gcgttggtca ggctctgcac gtagccacgc
     6661 acggcccagg tcgggatcac cgtcaggccg ccgcggatgc cgaagccgtg gcgcgtccag
     6721 gtgttcggcg gcggaggcgg gggcgcgagc gactcctccg gcggcggcgc gggcggagtg
     6781 accaccggct gcggctccgg ggcgggctgg tcgacgaggt ccttcgaggg cggcgccttg
     6841 gagctcgtcg gccgcagcga cgggtcggtc gcgggcgtcg gcgccggtgc gacggtcgtg
     6901 gtggtggtcg tcgtcgtcgt cgtgccgggg ctcgctgcac ccgcggggtc ctgcagggcg
     6961 tgcaggtggg cgtaggggct cgcctgggcc agcgacggga gcagggcgag ggacgaggcg
     7021 agcaagaagc caggacgcat ggtcggtact atagcgaacc gggggcccgc gcggcaaaac
     7081 ctgacgtggt tagcgatgcg cggaggcggt ccggcgcccg gccgcgggaa agtccccaca
     7141 gccggacgtg cgacgggccg ttcacaggcg ggattacagg cggatggtcc gccagccgcg
     7201 gccgaggaac ttgagggcca tcgccaggcc catcaggttg gtgtagaggg cggcggccag
     7261 ccacacgccg tcgaggccat agccgagcct ggggcccagg agccagctca gcggcacgag
     7321 cacgccgaag tgcaggatgc cctcgacgac catgacgaag gtgttggcgc cggcgccgta
     7381 gagggcctgc gagagcacga ggccgaccac caatgcgggc aggccggcgg cgacatagcg
     7441 cagggcctcg gcgccggctg cggcgacggc cgggtcgttg ggggcccaga tgcgaaggat
     7501 ctgctcgggg atggcgagga agatcgccgc gaccacgagc atcgcgtaga cgccgaggcg
     7561 caccgactcc cagccgtagc gggcggcgag gttgggcttg ccggcgccga ggctctggct
     7621 gacggcggtc gcggtcgcgg tgccgagcgc gatgactggc atgaagcaca tcgaggcggt
     7681 gtccatcagc gccttggtgg ccgcggagta ggtgttgccg ccgacgcgcg cgtcgatctc
     7741 accgacgaag cgcatgaaca gcatgaagcc gatcatcagg atgacggtcg cggagccgga
     7801 cgggagcatc aggcgcacga tgtcgcggac gatgctccag cgggtgtggg cccagcggta
     7861 gaactggtag cgccggctcg cggggccgag gctgatgccg aacatgatca gcaggccgac
     7921 gtaggtgctg acggccgagg ccaggccggc gccggtcagc gccatcgccg ggatcccgag
     7981 cgagggggcg ccgtagatga ggaggtagtt gaggccgatg ttgagcaggt tcatcaccag
     8041 cgcggcgtag aggtgcacgc gggtgcggcc gatgccgtcg aagaaggcct tgtagctgaa
     8101 ggtcaggacc atcgccggga tcccgagcat gcgcatgcgg gcgtaggaga tcccgagctc
     8161 ggcctgctcc gggctggcct tggccaggaa gtcgatcgcg gtcggcgtgg tgaaccagcc
     8221 gatcagaccc ccgacgagcc cggcgatcgt ggcgacgacg agcgagttcc acagcacctg
     8281 gccggcgcgc gcgtcatcac cctcggcgta gcggcgggcg gtcatcgcct gggtgccgaa
     8341 ggcgaccgcg cagaagaacc cgccgaccgc ccagaagaac ggcatgccga ggccgagcgc
     8401 cgcctgtgag gcggtggcga gcgcgtggtc gtcgagccgg ccgaccatga agttgtcggc
     8461 ggcgttgacc aggaactggg tgatcatcgc cagcatcacc cagctgccga gcgcccacac
     8521 gcggcgcacg agcgggagct cgaggcccag ttttaatagg cggggtgggt cgtcgccgag
     8581 gtcggtcagt tgcacggggc cgcgcaggtg tatgccatgc gcgggccgcc gacaagccgc
     8641 gactacttga gctgctcgag ctgcgtgcgg atgtcctgct cgagcttgcc ggcgcactcg
     8701 ggcttgtagc cgcgatacga gccgcggatc tggcccttgc cgtcgatcac gaaggagaac
     8761 gggaagttgc tgctctcgta gtcgtcggcg agctgctgtc caccgagcgc gagcttggtc
     8821 gccagcttgc gctccgccgc gaactcctgg accttctccg gctcggtgtc ggtggcgatc
     8881 gcgatcacga cgaggccgtt cttgcggtag cgccggtaca ggcggtcgaa ctcgggcagc
     8941 tccttgaggc agggcttaca ccacgtgccc cagaagttga gcagcacgac cttgcccctg
     9001 tgactggcga ggctgatgtc cttgccctcc ggcgttttta aattgaaggg cttcgccgcc
     9061 gtgccgacgc cggggtcgtt gccgcagcgc gcgtcgatct tggtgctcag cgccgcgggc
     9121 aagggctcct tgttggaagc gggcgtcggg gccgggccgg cgtcgatcgc cggggccggc
     9181 tcggtcgatc cggcagtggt cgcgacggtg gtggcggtgc cgggctcggc gggcatcggc
     9241 tcgcccgaca tcgtgttcgg cggctcgctc gtcggcgggc cggtgacctc gggcgcgacc
     9301 ggggccggcg tcggctcggc gggcggctgg gtcgcgggct cgggcttgca cgcgagcgtc
     9361 agggtcgcta gcgctgcgaa gatcttcgtg ttcatgtcga cacctccacg gacgcggaaa
     9421 cagcgcgcca gacctcgcgc agctcggcct ccccggcgcg gtacggcagg agcaggcgga
     9481 tcgtgtggcc gccgcacacg gtgacgagga tcgcgtggtc acgcatgagt ctgtcgccga
     9541 cggcggtgcc gatctgctcg cccggcttgc cgacctggat cccgatcatc gcaccgaggc
     9601 cgcggacctc gtgcacgcgg gcgctcggat catcggccgc gaggcgctgc agggtcggca
     9661 gctgggcggc ggcggtcgcc agcaggccct cggactcgat gatctcgatc gtcgccaggg
     9721 cggccgcgca ggcgagcggg ttgccgccga aggtggtccc gtgggtaccc ttgcccatcg
     9781 cctgcgcgag cgtggcggtc gtcaggcacg cgccgatcgg catgccgccg ccgagcgcct
     9841 tggccagcca gaggatgtcc ggctgcaagg cagggtcgtc gccgacgatg tgcgtccacg
     9901 ccgaccacgc gccgacccga ccgccaccgc actggatctc gtcggcgccg agcagcgcgc
     9961 cgtgctcgcg gcacaggccg tgcaggccga ccaggaattc acgcgacccg gggtacacgc
    10021 cggcctcgcc ctgcaccggc tcgacgaaca cggcggcgat gtccttgccg tgcttcgcga
    10081 acgcggctcg cacggcgtcg aggtcgtccc acggcaggcg aagcacctgc gcggtggcga
    10141 aggggcgctc gggggcggcc tcgtcgaggc agcgcacgta ggggtggcga taggcggtgt
    10201 tggcggtcag cgagagggcg ccgaggctgc gcccgtggaa ggcgcgctcg aaggcgacga
    10261 tgatgctgcg cccggtcgac cgcaccgcga gcttgatcgc ggcctcggtg gcctcggtgc
    10321 cggagctgcc gaggaacacg cgggcgtcgc gaattggaaa gcgctcggcg agcttgtgcg
    10381 cgagcacctg ctgcggcgcg gtggtgaagc tgttggcggc cgaggtcagt cgttgcagct
    10441 gcgcgtgcac gtcggcgacc cagcgcgggt ggttgtggcc cagcggcgca cagccgacgc
    10501 cgttgaccgc gtcgagatag ctgcgcccct cgctgtcgat cacccgcagc gcgccgtgct
    10561 catcaggctc ggcgcggtcg agggtgacgt cgcggagcgc ataggtcggc caccggaggt
    10621 cctgcatggg ggaagtgttg gcaggcgggc gcggcggatg caagcacttc gcccgcgcac
    10681 cgggccgcta gaacgcgagc tgcaggcccg aaagggtgat acgcacgcgc tcgcggccgg
    10741 cctttcgccg gtcgcgcagc gcggcgatcc cgagcgcgag cgtgatcacg gcgagccccg
    10801 cggtgacgcc gaccatcacg ttggtggcgt cgcggtagcg gcggaacgag ccggccgtgt
    10861 cggtgcaggt gccggtcctg ccgcaatcct cgtgcacggc gcgctggcgc aacaccagcc
    10921 cgccgagcac gcccatggcg atcgcgctgg cggtcgtcag cccggtgccg gtccagaaca
    10981 gcgcggtcag cgggcgcacg cgcggcgcgg gggggaccgg cgtcgggtcg gggcggacga
    11041 cgcgggtcgt cggtctcggg acgacgggct cgggcgcctc gggtcgggcg aagccggtga
    11101 actgcacggt ccaggtgccg ccgggcgcga cctcggcggg gatgtcggcg gtctcgccgg
    11161 gcccggcacc gatcaatcgc agctcgacgt gaccgggcgg cacgagcttc gggaagtcgg
    11221 agagggtgac cgcctcacca ttgacgcgga tctcgcggat ctcgtcggtg ctgtcgacct
    11281 gcaggcgcag ctcgccgagg cgcgcgcgca gctcgagcgc ccgctgctgc gcctgcgggt
    11341 agctcgggtc ctcggggctc gcggtcgcca ggtacctgcg ataccagcgc atggtcgcga
    11401 ggtcgtcacc gaccttgtcg tgggcgagcg cggcgttgta ggcggcctcg ggcgaggccg
    11461 cggccgcgta cgaggcctcg aagccgcgag ccgccgccgc gtaggcgccg gcgctgtaca
    11521 gctcgcgggc ggcacgaaac tggcggatcg cctcgtcctg cccgctcggc ggggggtcgg
    11581 atgcatccgc ggtggcaggt gggtccggtg cctgcgcggc gtgggccggg gtcgcaaggg
    11641 cgaccg
//
LOCUS       DASWNJ010001746         1551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_382557, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001746 DASWNJ010000000
VERSION     DASWNJ010001746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382557"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VGB85_29140"
     CDS             <1..536
                     /locus_tag="VGB85_29140"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY0138198.1"
                     /translation="ARRSTPACALAYAARAMHRLEISADLGKPETYAELLASLRAVVE
                     DEPDLIANLANVTAVLKQGLADVSWVGFYRQIGSDLVLGPFQGKLACTRIAAGKGVCG
                     AAAARRATLVVPDVDAFPGHIACDAGSRSEIVVPIVHAGAVVAVLDLDSEQYARFDAV
                     DAVHLEQVAALVAGLTW"
     gene            548..1447
                     /locus_tag="VGB85_29145"
     CDS             548..1447
                     /locus_tag="VGB85_29145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138199.1"
                     /translation="MIIVTGTKRSGTSMWMQILIGAGYPIIGEAFPMRWEHTIKAANP
                     EGFYESHLRRGIYFRTNPHPKTGSYLFPEQVAHHAVKVFIPGLARTDRAYIGKVIATV
                     REWREYESSIRRLYAIEDESRRNEGKGAFIPEERMPGALEWWAENFALVRDIAVRRYP
                     VHVQTYEGLLADPEKVIRDVIKWLGRGDADKGLAAVKPEHRTQNRPPSDTVEPEIAEV
                     FDAFYAAMHAGKGLDAALLQKLNDTNEILAPRIGQLHARLREQNRKRLAARAAFRREQ
                     AAAGKPVTTDDSTHEDDETEEPG"
BASE COUNT          245 a          610 c          494 g          202 t
ORIGIN      
        1 gcgcgcgccg gtcgaccccg gcgtgcgcgc tggcctatgc tgcgcgcgcc atgcatcgcc
       61 tcgagatctc cgccgacctc ggcaagcccg agacctacgc cgagctgctc gccagcctgc
      121 gcgcggtcgt cgaggacgag cccgacctga tcgccaacct cgccaacgtc accgccgtgc
      181 tcaagcaggg cctcgccgac gtctcgtggg tcggctttta ccggcagatc ggttccgacc
      241 tggtcctcgg gccgtttcag ggcaagctcg cgtgtacgcg catcgccgcg ggcaagggcg
      301 tgtgcggggc ggcggcggcc cggcgcgcga ccctcgtcgt gcccgacgtc gacgccttcc
      361 ccggccacat cgcctgcgac gccggctcgc gcagcgagat cgtcgtcccg atcgtgcacg
      421 ccggggcggt cgtcgccgtg ctcgacctcg acagcgagca atacgcccgc ttcgacgcgg
      481 tcgacgcggt ccacctcgag caagtcgccg cgctggtcgc cggcctcacc tggtaggatc
      541 cgccgcgatg atcatcgtca ccggcaccaa gcgctccggg acctcgatgt ggatgcagat
      601 cctcatcggc gccggctacc cgatcatcgg cgaggcgttc ccgatgcgct gggagcacac
      661 gatcaaggcc gccaacccgg agggcttcta cgagtcgcac ttgcgccgcg gtatttactt
      721 ccgcaccaac ccccacccca agaccggctc ctacctgttc ccggagcagg tcgcccacca
      781 cgcggtcaag gtgttcatcc ccgggctcgc ccgcaccgac cgcgcgtaca tcggcaaggt
      841 gatcgccacc gtccgcgagt ggcgcgagta cgagtcgtcg atccgtcgcc tctacgcgat
      901 cgaggacgag tcgcgccgca acgagggcaa gggcgcgttc atcccggagg agcgcatgcc
      961 cggcgcgctc gagtggtggg ccgagaactt cgcgctggtc cgcgacatcg ccgtgcgtcg
     1021 ctaccccgtg cacgtgcaga cctacgaggg cctgctcgcc gaccccgaga aggtgatccg
     1081 cgacgtcatc aagtggctcg gccgtggcga cgccgacaag ggcctcgccg ccgtcaagcc
     1141 cgagcaccgc acccagaacc gtccgccctc cgacaccgtc gagcccgaga tcgccgaggt
     1201 cttcgacgcc ttctacgcgg cgatgcacgc cggcaagggc ctcgacgccg cgctcctgca
     1261 aaagctcaac gacaccaacg agatcctcgc cccccgcatc ggccagctgc acgcccgcct
     1321 gcgcgagcag aaccgcaagc gcctcgccgc ccgcgccgcc tttcgccgcg aacaggcggc
     1381 cgccggcaag ccggtcacga ccgacgacag cacccacgag gacgacgaga ccgaggagcc
     1441 cggctgagcc gccccgcgcc gcggcgagcg ctcacaacgt cgtcacaccg agcaccaggc
     1501 cgagctgcgg cagcggggca aactcgccgt agccgacgtt ccagccgagc c
//
LOCUS       DASWNJ010001747         1771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_382564, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001747 DASWNJ010000000
VERSION     DASWNJ010001747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382564"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="VGB85_29150"
     CDS             <1..916
                     /locus_tag="VGB85_29150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEY0138200.1"
                     /translation="GLKAVMMAAQAIRAGDAEIIVAGGMESMSNVPYLLPQARTGYRL
                     GNGALIDGLVHDGLWDPYNNFHMGSAGELCARECQIPRDRQDAYAADSYRRGQAAVKE
                     GKFRDEIVAVEVPARKGPPTTVSDDEQPFKGDPDKLASLRPAFDKNGTITAGNASTLN
                     DGAAATVIMSREEADRRGIVPLATIKGYTGHAQAPEWFTTAPAGAITSLLQKTGLTAN
                     DVDLWELNEAFAVVALVNQDKLGIPHERLNVRGGAIALGHPIGCSGARVLTTLLYALK
                     QDGKRRGVASLCIGGGEAVALMVEMNAR"
     gene            932..>1771
                     /locus_tag="VGB85_29155"
     CDS             932..>1771
                     /locus_tag="VGB85_29155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY0138201.1"
                     /translation="MTTIDLDTIKSIGVLGAGQMGAGIAQVAAQGGYEVLLADVSQER
                     ADAGRNGIGKQLTRLVEKGKLGADARDAALARITAVVGAAGLAPADFLVEAATENIDL
                     KLKLFIELDRAARPGVILASNTSSISITKLAAATSRPDHVVGMHFMNPVPLMKLVEII
                     RGLQTSDPVYQVTVALAQKLGKTTITSKDCPGFLVNRILIPLINEAGFALQEGLGTPE
                     DIDTGAKLGLNHPMGPLELADLIGLDTCLAIADVLHRELGDDKYRAPNILRMHVAAGW
                     FGRK"
BASE COUNT          309 a          638 c          608 g          216 t
ORIGIN      
        1 cgggctcaag gccgtgatga tggccgcgca ggcgatccgc gccggggacg cggagatcat
       61 cgtcgcgggc ggcatggaga gcatgagcaa cgtgccctac ctgctcccgc aggcgcggac
      121 cggctaccgc ctcggcaacg gcgcgctgat cgacgggctg gtgcacgacg gcctgtggga
      181 cccgtacaac aacttccaca tgggcagcgc cggcgagctg tgcgcgcgcg agtgccagat
      241 cccgcgcgac cgccaggacg cctacgcggc cgacagctac cgccgcgggc aggcggccgt
      301 gaaggagggc aagttccgcg acgagatcgt cgccgtcgag gtgccggcgc gcaagggccc
      361 gccgacgacc gtcagcgacg acgagcagcc gttcaagggc gaccccgaca agctcgcctc
      421 gctgcgcccg gcgttcgaca agaacggcac gatcacggcc ggcaacgcct ccaccctcaa
      481 cgacggcgcg gccgcgacgg tcatcatgtc gcgcgaggag gccgaccgcc gcggcatcgt
      541 cccgctggcg acgatcaagg gctacaccgg ccacgcccag gcgcccgagt ggttcacgac
      601 ggccccggcc ggcgcgatca ccagcctgct gcaaaagacc gggctgaccg cgaacgacgt
      661 cgacctctgg gagctcaacg aggcgttcgc cgtcgtcgcg ctggtcaacc aggacaagct
      721 cggcatcccg cacgagcgcc tcaacgtgcg cggcggcgcg atcgccctcg ggcacccgat
      781 cggctgctcg ggcgcgcgcg tgctgacgac cctgctgtat gccctcaagc aggacggcaa
      841 gcgccgcggc gtcgcgtcgc tgtgcatcgg cggcggcgag gccgtcgcgc tgatggtcga
      901 gatgaacgct cgctaaacgc aggaacaaca catgacgacc atcgacctcg acacgatcaa
      961 gtccatcggc gtcctcggag ccggacagat gggcgctggt atcgcccagg tggccgcgca
     1021 gggcggctac gaggtgctcc tcgccgatgt cagccaggag cgggccgacg ccggccgcaa
     1081 cggcatcggc aagcagctga cgaggttggt cgagaagggc aagctcgggg ccgacgcccg
     1141 cgacgccgcg ctcgcgcgca tcacggcggt ggtcggcgcg gcgggcctgg ccccggcgga
     1201 cttcctcgtc gaggcggcga ccgagaacat cgacctcaag ctcaagctgt tcatcgagct
     1261 cgaccgcgcg gccaggcccg gcgtgatcct ggcgagcaac accagctcga tcagcatcac
     1321 caagctggcc gcggcgacca gccgccccga ccacgtggtc ggcatgcact tcatgaaccc
     1381 ggtcccgctg atgaagctcg tcgagatcat ccgcggcctg cagaccagcg acccggtcta
     1441 ccaggtcacg gtggcgctgg cgcagaagct cggcaagacg acgatcacca gcaaagactg
     1501 cccgggcttt ttggtcaacc gcatcctgat cccgctgatc aacgaggcgg gcttcgcgtt
     1561 gcaggagggg ctcggcacgc cggaggacat cgacaccggc gccaagctcg ggctcaacca
     1621 cccgatgggt ccgctcgagc tcgccgacct gatcggcctc gacacctgcc tggcgatcgc
     1681 ggacgtgctg caccgcgagc tcggcgacga caagtaccgt gcgcccaaca tcctgcgcat
     1741 gcacgtcgcg gccggctggt tcggccgcaa g
//
LOCUS       DASWNJ010001748         9434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_242991, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001748 DASWNJ010000000
VERSION     DASWNJ010001748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 9434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9434
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242991"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VGB85_29160"
     CDS             <1..207
                     /locus_tag="VGB85_29160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0138202.1"
                     /translation="MDANLVGGDLGGGSNAWNRQLVFRPLFPYFRHRMPVRRLYLCSS
                     YTHPGSGVHGMCGYNAARIALRDL"
     gene            269..1459
                     /locus_tag="VGB85_29165"
     CDS             269..1459
                     /locus_tag="VGB85_29165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEY0138203.1"
                     /translation="MRGMNGLGRTSLIFLSVLAGCGGGTGTSATESGGGTDDGSTSQG
                     TQTSQQTTTGEASESAGPTTSGGSQGSQTGTTTDAPTTGETVSASGTDTGTTTNETSF
                     STDQMTGGSTMEATISESGTTGDDGTSSGSGSTTTTGGESTTDAESSTGPGCVPTEEV
                     CNDIDDNCDDQIDNVDVGKDGICDCLSIALLGNQGANPSAEFQSWLEAQGTEVDRISI
                     NPAEVLDKATLNKYDIIVLDYLVRSYTVEEQAAVQTWVEAGGGMMAMTGFTNAPFVAD
                     RPNSLFQPMGLTYNTSKGFFSGPVTNFAPHEITKDLTSISFYGGLFVNIVEDGIGVNK
                     VIMTLPQGPVGVVQDRGVGRLFMFGDEWIEFDSEWKNIPQIKQFWVQTLSFLGPKESC
                     QVPQ"
     gene            complement(1501..3336)
                     /locus_tag="VGB85_29170"
     CDS             complement(1501..3336)
                     /locus_tag="VGB85_29170"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0138204.1"
                     /translation="MRPTLTTLSAAFLLQFLLPSGAAHAAPWEDATAKTIGVTAEWSN
                     KVELADINGDGLVDILFANGAGYSTPGGAEQSRVFVNQGAGKPFMDVSAEVFPDPGQT
                     RVIKVRDLDADGNVDIVVGNTFETQSWLYMGDGMGGFIDATDKLPQKPASIGDLEFGD
                     VDGDGDLDIVLADWGPGKASQSAGVTQLWLGDGLGGFTDATASNMPDIPVAWSWEMEL
                     ADVDNDHDLDVLVSCKSCTGSYMFHNDGMGMFTDASAQMPQFANNYEFEAIDLNGDDF
                     LDLITVNDGPEFREHIFVGDGMGGFTDATAELWPDIQQPPGDDNMIAYLDVESDGDAD
                     FVICGLFGGLDRLLVNDGKGALTLEATAFAPATSSGSLGIAVADLNGDKKLDVVFAEG
                     EAPDEADRVFLGVDVPVDTAAPKISLVDIEVGGNAITARARIHDNKSPVMPHDFTSVE
                     LTLYGDGAQAVFPMIWYGEGLWRAFDDFAGSEHDLAIICAVDAAGNEACSDPVRLNPG
                     EDTDTDPGVETSSGTTSGTTDVTTNATGETGETPTTGDMQTSADSAMTEASGNITASA
                     GDSSGDTSGGEDKNEGGCGCRSEPTTGGAWLLALGGLLLVRRRRG"
     gene            complement(3370..4467)
                     /locus_tag="VGB85_29175"
     CDS             complement(3370..4467)
                     /locus_tag="VGB85_29175"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0138205.1"
                     /translation="MALRLIIEDEEGATTIVPLGEEEVTIGREPGNTIQLTEQNVSRQ
                     HARLAQGPDGWVIEDLESYNGVKVNGVLVEGSVTLNEGDVVQIGDYHLALSNNVEQTT
                     LNIDRPGQAANEPNRVMPSVDLPRISASEAAAIATPSFVGGDGRGEAPHDDEREGGGK
                     GLWLGLGLVALLGVGFLVFRMMGPGQTPPKSDKGVAAAVGPVETKTPTKAITPEPPPQ
                     PEPEPKPEPTPPDASAGDVAPPDASAGDEEPEPEEPAKTVRTPKPVTPKPAPVDKPKP
                     KPEAPKGDPVALLEEARKASMANNATQAYDLARKSYDIDRNGAALKLMGLSACKMGDQ
                     SKAKSAYKKLDSALQKQLQSVCQGMGITLEP"
     gene            complement(4683..5618)
                     /locus_tag="VGB85_29180"
     CDS             complement(4683..5618)
                     /locus_tag="VGB85_29180"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HEY0138206.1"
                     /translation="MSAAPEERDRALRISLAAWVVVACLLLLMAPPPPPPPVTTAEDE
                     AIFQRTVDQTVEKTRRWQAERGDQTIAWADAKGHLAIVIDDVGRELHLFEQLLALRFP
                     LSFAVLPGAVYAPGVQLRLRGDRRRPRDILLHLPCEPEDAAKMTADAAEGEQFLRVGD
                     PPERLRSALVAALARVPAAVGVSNHMGSRLTAERAAMDALMPELRARGLFFFDSMTTP
                     ASEVGPAAEAAGVPTLARDIFLDVDPTPGAVLAQLELAAQRARDRPVVIIAHPSQAVV
                     EVLREALPRLHRDGIGVYPLREILARRREASAQQH"
     gene            complement(5623..7029)
                     /locus_tag="VGB85_29185"
     CDS             complement(5623..7029)
                     /locus_tag="VGB85_29185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0138207.1"
                     /translation="MPRASRLLTSARLRAAALLLTGMAFGAGLMLLRGAEAGPAEQVK
                     PAPLDYSRYRKLDLFARSLAIVEQHYVRPVDGERMIYAAITGLVSELDPHSEFLPPQD
                     AKLLREDIEGSFGGIGTVIVLRQETQAPARVVLEVREVIAGGPAARAGVKIGDYITEI
                     EGKPIGHFFDMRRALSAMRGPPGSKVSFTLETPQAKGKPVVRPLTVTREFIDSPAVIP
                     AYLGEGMAHVRLRDFSETSAHELSEAIAKMREAAGKAGLQGVVLDLRDNGGGLLDQAV
                     RVVDLFVDAGPIVRTRGRMGNLLDESRALPGGPWNKVPLAVIINKASASASEVVAGAL
                     QDHHRALIVGERSYGKGSVQAPFELGDGSVLKLTISLYYTPADRLIQASGILPDVLVG
                     TDMAEYKDSLPELEPERAQPRHLRPEAFGHPASTQGDESEAVRAAGTDAQLRAAVQHL
                     RSHIYLQSRTTPRSPRRG"
     gene            complement(7040..7255)
                     /locus_tag="VGB85_29190"
     CDS             complement(7040..7255)
                     /locus_tag="VGB85_29190"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEY0138208.1"
                     /translation="MKLPVILRPGEDGWLVAECPVIPGCISQGKTREEALDNIREAIE
                     LCLETREEEASWSLPQRYEVVDVEFAA"
     gene            complement(7579..>9434)
                     /locus_tag="VGB85_29195"
     CDS             complement(7579..>9434)
                     /locus_tag="VGB85_29195"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971064.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEY0138209.1"
                     /translation="PNRETMLRVIRNHRRAAYDVARSEYEGLSTAPRGIDQRMCPPYL
                     LKAAQQEWDTALQLGEQHGYRNAQVTVIAPTGTIGLLMDCDTTGIEPDFALVKFKKLA
                     GGGYFKIINQCVPAALATLGYTDTQITDIVRYCKGAGTLVGSPRISHADLKRKGFTDE
                     VLQRVESELAGAFEIGFVFNRYVLGDDFCHGVLGFTDAQLGNPAFSVLEALGFSVEDI
                     QRANDYVCGTMTVEGAPHLKSQHLPVFDCASRCGRYGRRFIAPMAHVRMMAAAQPFLS
                     GAISKTINMPSDATVTEVGDVYLASWKYGVKANALYRDGSKLSQPLSSGLVVEAEDGT
                     EQTLSTSQPQVIEKITERIVHRYLAKRRRMPDRRAGYTQKATIGGHKVYIHTGDYSDG
                     TLGEIFIGMHKEGAAFRSLMNCFAIAISLGLQYGVPLEEFVDAYVFTRFEPNGSVQGH
                     PRIKMSTSIIDYIFRELAVTYLGRNDLAHVNPEDLEPDSIGGESDSNQIEFAEEEVVA
                     ERIVTPGPLKLSVDVPRAAQVYANKARRQPIEVEDRTAAAAAAAPAAPAKARAPAPAV
                     VAAPPSVSEQIRVARMQGYEGDACGACGSFTMVRNGSCLKCVSCGGTSGCS"
BASE COUNT         1370 a         3181 c         3364 g         1519 t
ORIGIN      
        1 atggacgcca acctcgtcgg cggcgacctc ggcggcgggt cgaacgcctg gaaccggcag
       61 ctcgtgttcc gcccgctgtt cccctacttt cgccatcgca tgccggtgcg ccggctgtat
      121 ctgtgctcga gctacacgca cccgggcagc ggcgtgcacg gcatgtgtgg ttacaacgca
      181 gcccgaatcg cgctgcgcga tctgtagcgt gtgtggacag cacagcgatg cgcggggggc
      241 tccggccccc gcgtgttagc ctgcggtcat gcgtggaatg aatgggttgg ggcggacttc
      301 tttaatcttt ttgagcgtgc tcgctggctg cggcggaggt acgggcacat cggcgaccga
      361 gagcggcggc ggcacggacg acgggtcgac ctcgcagggg acgcagactt cgcagcagac
      421 gaccaccggt gaggcgagcg agtcggcggg gccgaccacc tccggtggca gccagggctc
      481 gcagacgggc acgaccacgg acgcgccgac gaccggcgag accgtctcgg cgtccggcac
      541 ggacaccggc acgaccacca acgagaccag tttctccacc gatcagatga ccggcggctc
      601 gacgatggag gcgacgatca gcgagtcggg cacgaccggc gacgacggca cgagctccgg
      661 gtccggcagc acgacgacga ccggcggcga gtcgacgacc gacgccgaga gcagcacggg
      721 tcccggctgt gtgcccacgg aggaggtgtg caacgacatc gacgacaact gcgacgacca
      781 gatcgacaac gtcgacgtcg gcaaggacgg catctgtgac tgcctcagca tcgcgctgct
      841 cggcaaccag ggcgccaatc cctcggccga gttccagagc tggctcgagg cccagggcac
      901 ggaggtcgac cgcatcagca tcaacccggc ggaggtgctc gacaaggcga ccctcaacaa
      961 gtacgacatc atcgtcctcg actacctggt ccgcagctac acggtcgagg agcaggccgc
     1021 cgtgcagacc tgggtcgagg ccggcggcgg catgatggcg atgacgggct tcacgaacgc
     1081 gccgttcgtc gccgaccgcc ccaactcgct gttccagccg atgggcctga cctacaacac
     1141 aagcaagggc ttcttcagcg gtccggtcac caacttcgcg ccgcacgaga tcaccaagga
     1201 cctgacctcg atctcgttct acggcggcct gttcgtcaac atcgtcgagg acggtatcgg
     1261 cgtcaacaag gtgatcatga ccctgccaca gggccccgtc ggcgtggtcc aggaccgcgg
     1321 cgtggggcgc ctgttcatgt tcggcgacga gtggatcgag ttcgactcgg agtggaagaa
     1381 catcccccag atcaagcaat tctgggtgca gacgctgtcg ttcctcggac cgaaagagtc
     1441 ctgccaggtc ccgcagtgaa gcgctgagcg gagcgccgcg agggcgctcc gctcgtcacc
     1501 tcagccgcgg cgacggcgga cgagcagcag gccgccgagc gccagcaacc aggcgccgcc
     1561 ggtcgtcggc tcgctgcggc agccgcaacc gccctcgttc ttgtcctcgc cgccggaggt
     1621 gtcgcccgag gagtccccgg cgctggcggt gatgttgccg gaagcctcgg tcatcgcgct
     1681 gtcggcggag gtctgcatgt cgcccgtggt cggcgtctcg ccggtctcgc cggtcgcgtt
     1741 ggtggtgacg tccgtggtcc cgctggtcgt cccgctgctg gtctcgacgc cagggtcggt
     1801 gtccgtgtcc tcgcccgggt tgagcctgac cggatcgctg cacgcctcgt tgccggccgc
     1861 atcgacggca cagatgatcg ccaggtcgtg ctcgctgccc gcgaagtcgt cgaaggcgcg
     1921 ccacaggccc tcgccgtacc agatcatcgg gaacacggcc tgggcgccgt ccccgtagag
     1981 ggtgagctcg acgctggtga agtcgtgcgg catgacgggc gacttgttgt cgtggatgcg
     2041 ggctcgggcg gtgatggcgt tgccgccgac ttcgatatcg acgagcgaga tcttcggcgc
     2101 ggcggtgtcg accggcacgt cgacgccgag gaacacgcgg tcggcctcgt ccggcgcctc
     2161 gccctccgcg aacacgacgt cgagcttctt gtctccgttg aggtcggcga cggcgatgcc
     2221 gaggctgccg ctgctggtcg caggcgcgaa cgcggtcgcc tccagggtca gcgcgccctt
     2281 gccgtcgttg accagcaggc ggtcgaggcc gccgaacagg ccgcagatga cgaagtcggc
     2341 gtcgccgtcg gactcgacgt cgaggtacgc gatcatgttg tcgtcgccgg gtggctgctg
     2401 gatgtcgggc cacagctcgg cggtcgcgtc ggtgaagccg cccatgccgt cgcctacgaa
     2461 gatgtgctcg cggaactccg gcccgtcgtt gacggtgatc aggtcgagga agtcgtcgcc
     2521 gttgaggtcg atcgcctcga actcgtagtt gttggcgaac tgcggcatct gggcgctcgc
     2581 gtccgtgaac atacccatgc cgtcgttgtg gaacatgtac gagcccgtgc acgacttgca
     2641 cgagaccagc acgtcgaggt cgtggtcgtt gtcgacgtcc gccagctcca tctcccatga
     2701 ccacgcgacc gggatgtccg gcatgttgct cgcggtcgcg tcggtgaagc cgccgaggcc
     2761 gtcgccgagc cacagctgcg tcacgccggc cgactggctg gctttgccgg ggccccagtc
     2821 ggcgagcacg atgtcgaggt cgccgtcgcc gtcgacgtcg ccgaattcga ggtcgccgat
     2881 gctcgcgggc ttttgcggga gcttgtcggt ggcgtcgatg aagccgccca tgccgtcgcc
     2941 catgtacagc cagctctggg tctcgaaggt gttgccgacg acgatgtcga cgttgccgtc
     3001 ggcgtcgagg tcacgcacct tgatcacgcg ggtctggccc gggtcgggga acacctcggc
     3061 gctgacgtcc atgaacggct tgccggcgcc ctgattgacg aacacgcggc tctgctcggc
     3121 gcccccgggc gtgctgtagc cggcaccgtt ggcgaacagg atgtcgacca ggccgtcgcc
     3181 gttgatgtcg gcgagctcga ccttgttcga ccactccgcg gtcacaccga tcgtcttcgc
     3241 agtcgcgtcc tcccatgggg ccgcgtgcgc ggcgcccgac ggaaggagga attgcaggag
     3301 gaaggccgca gagagggtcg tcagcgtagg tcgcatgcgc gagccctacc acggaccagc
     3361 gcggccgagc tacggctcga gcgtgatgcc catcccctga cacacggact gcagctgctt
     3421 ctggagcgcc gagtcgagct tcttgtaggc cgacttcgcc ttcgactggt cgcccatctt
     3481 gcaggccgag aggcccatca gcttgagcgc cgcgccgttg cggtcgatgt cgtagctctt
     3541 gcgcgcgagg tcgtaggcct gcgtcgcgtt gttggccatg ctcgccttgc gcgcctcttc
     3601 gagcaacgcc accgggtcgc ccttgggcgc ctcgggcttg ggcttgggct tgtcgaccgg
     3661 cgccggcttg ggcgtgaccg gcttcggcgt gcgcaccgtc ttggccggct cctcgggctc
     3721 cggctcctcg tcaccggcgc tcgcgtcggg cggggcgacg tcgccggcgc tcgcgtcggg
     3781 cggcgtcggc tccggcttgg gctccggctc cggctgcggc ggcggctccg gtgtgatcgc
     3841 cttggtcggg gtcttggtct cgacggggcc gaccgccgcc gccacgccct tgtcggactt
     3901 cggcggagtc tggccggggc ccatcatgcg aaacacgagg aagccgacgc cgagcagcgc
     3961 gacgagcccg agcccgagcc acagaccctt gccgccgccc tcgcgctcgt cgtcgtgggg
     4021 cgcctcgccg cggccgtcac cgccgacgaa gctcggcgtg gcgatcgcgg cggcctcgct
     4081 cgcggagatc cgcgggaggt cgacgctcgg catcacccgg ttcggctcgt tggcggcctg
     4141 acccgggcgg tcgatgttga gtgtggtctg ctcgacgttg ttgctgagcg cgaggtggta
     4201 gtcgccgatc tgcacgacgt cgccctcgtt gagggtgacc gacccttcga cgagcacgcc
     4261 gttgaccttg accccgttgt agctctcgag gtcctcgatc acccagccgt cgggaccttg
     4321 agcgagacgc gcgtgctggc gagagacgtt ctgctcggtg agctggatgg tgttacccgg
     4381 ctctcgcccg atcgtgacct cctcctcgcc gaggggcaca atcgtcgtcg caccctcttc
     4441 gtcctcgatg atcagtcgca gggccatcgc gtgagcttta gggtaggaag cggatcgaat
     4501 tgctgtcaag ccaggacagt gccacagatg ccccggccag atcgcctcgc gcacgccgtc
     4561 ggctccaggg gtccccgtgc gtctcacccc cgtccgggcg tgcagcgccg aacagcccca
     4621 cacgaccgct cccgggccgg ggctccgccg ggtcccccag atccctctgc accgggaaaa
     4681 attcagtgct gttgcgcact tgcctcgcgg cggcgggcca ggatctcgcg caggggatag
     4741 acaccgatcc cgtcgcggtg caggcgcggc agggcctcgc gcaggacctc taccacggcc
     4801 tgggacggat gggcgatgat caccacgggc cgatcccggg cgcgctgcgc cgccagctcg
     4861 agctgggcca agacggcccc cggcgtggga tctacgtcga ggaagatgtc tcgcgcgagc
     4921 gtcggtacgc ccgcggcctc ggccgccggc cccacctcgc tcgccggcgt cgtcatcgag
     4981 tcgaagaaga acaggccgcg cgcccgcagc tccggcatca acgcgtccat cgcggcgcgc
     5041 tcggccgtca gccgtgaacc catgtgattg ctgaccccga ccgccgcggg cacgcgcgcg
     5101 agcgctgcca ccagcgccga ccgcagccgc tccggcgggt caccgacgcg caggaactgc
     5161 tcgccctcgg ccgcgtcggc ggtcatcttc gcggcatctt cgggctcgca gggcaggtgc
     5221 agcaagatgt cgcgcgggcg ccgtcggtcg ccgcgcaggc gcagctgcac gccgggggca
     5281 tacaccgcgc cgggcagcac ggcgaagctc agcgggaagc gcagcgcgag cagctgctcg
     5341 aagagatgga gctcgcggcc gacgtcgtcg atgacgatcg ccaggtgtcc cttggcgtcg
     5401 gcccacgcga tcgtctggtc gccgcgctcc gcttgccagc gccgcgtctt ctcgacggtc
     5461 tggtcgaccg tgcgctgaaa gatcgcctcg tcctcggcgg tcgtcaccgg cggaggtgga
     5521 ggcggcggcg ccatcaacag cagcaggcag gccaccacga cccacgcagc gaggctgatg
     5581 cgcagcgcgc ggtcgcgctc ctcgggggct gcggacacgc ggtcagcctc gacggggcga
     5641 gcgcggggtg gtccgcgact ggaggtagat gtgcgatctg aggtgctgga ccgcggcgcg
     5701 cagctgcgcg tcggtgcccg cggcgcgcac ggcctcgctc tcgtcgccct gcgtgctcgc
     5761 ggggtgaccg aaggcctcgg gccgcaggtg tcgcggctgg gcgcgctcgg gctcgagctc
     5821 cgggaggctg tccttgtact cggccatgtc ggtgccgacg agcacgtccg gcaagatgcc
     5881 gctcgcctgg atcaggcggt cggcgggcgt gtagtacagc gagatcgtca gcttgagcac
     5941 cgagccgtcg ccgagctcga agggcgcctg caccgagccc ttgccgtagc tgcgctcgcc
     6001 gacgatcagc gcccggtggt gatcctgcaa cgctccggcg acgacctcgg aggccgaggc
     6061 cgaggcctta ttaattatta cggcgagcgg gaccttgttc cacgggcccc cgggcagcgc
     6121 ccgagattca tcgagcaggt tgcccatgcg cccgcgcgtg cggacgatcg ggccggcgtc
     6181 gacgaacagg tcgacgaccc gcaccgcctg gtcgagcagg ccgccgccgt tgtcgcgcag
     6241 gtcgagcacg acaccttgca ggccggcctt gccggcggcc tcgcgcatct tcgcgatcgc
     6301 ctccgagagc tcgtgcgcgc tggtctcgga gaagtcgcgc aggcgcacgt gggccatgcc
     6361 ctcgccgaga tacgcgggga tcaccgcggg gctgtcgatg aactcgcggg tgacggtcag
     6421 cggccgcacg accggcttgc ccttcgcctg cggggtctcc aaagtgaagc tgaccttcga
     6481 gccaggtggt ccgcgcatcg ccgacagagc gcgccgcatg tcgaagaagt gcccgatcgg
     6541 cttgccttcg atctcggtga tgtagtcgcc gatcttgacg ccagcgcgcg ccgccggacc
     6601 gccggcgatc acctcgcgga cctcgagcac gacccgcgcc ggcgcctgcg tctcctgacg
     6661 caggacgatc accgtgccga tgccgccgaa gctgccctcg atgtcctcgc gcagcagctt
     6721 cgcgtcctgc ggcggcaaga actcgctgtg cgggtcgagc tccgacacga gccctgtgat
     6781 cgcggcgtag atcattcgct cgccgtcgac cggacgcacg tagtgctgct cgacgatcgc
     6841 cagcgagcgc gcgaacagat cgagcttgcg atagcgcgag tagtcgagcg gcgccggctt
     6901 cacctgctcc gccggccccg cctcggcccc gcgtagcagc atcagacctg ccccgaaggc
     6961 catgccggtc agcagcaagg cggccgcccg caggcgagcg ctggtcagaa gtcgcgacgc
     7021 gcgcggcatg gcgagcgtcc taggcggcga actcgacatc cacaacttcg tagcgttgag
     7081 gcaacgacca tgacgcttct tcctcgcggg tctcgaggca gagctcgatc gcttcgcgga
     7141 tgttatcgag cgcctcctcc cgagttttgc cctgagagat gcagccgggg atcaccggac
     7201 actcggcgac gagccagcca tcttcaccgg ggcgcagaat gacggggagc ttcatgagcc
     7261 ctgagcgtat acgccatggc ccggaacttc aaagcccccg tgccgtgagg cgcgggggcg
     7321 atccacctca gcgcgaacag agcgcgagga actcctccgg tgtcagctct gcggttcgca
     7381 aaatgctacg cagggtcccc cggtcgagag tcgagtgaag tgggacggtc acgggatttc
     7441 ggccctcgca gacgaggcga acatgactgc cttttgccgc agctcccgat agccgacggc
     7501 gcaaagggca gagatgcact gttttcctga gaggagggga agcgcgggca tggccaatcc
     7561 ttggctctga gccctggctc aggagcagcc gctggtgccg ccgcagctga cgcacttgag
     7621 gcaggagccg ttgcggacca tcgtgaagct gccgcaggcg ccgcaggcgt cgccctcgta
     7681 gccctgcatg cgggcgacgc ggatctgctc gctcacggag gggggcgcgg cgacgacggc
     7741 cggggccggg gcgcgcgcct tggccggggc ggccggggca gccgcagcgg cggccgcggt
     7801 gcggtcctcg acctcgatcg gctggcggcg cgccttgttg gcgtagacct gcgcggcgcg
     7861 ggggacatcg acgctgagct tgagcgggcc cggggtgacg atgcgctcgg cgacgacctc
     7921 ctcctcggcg aactcgatct ggttcgagtc cgactcgccg ccgatgctgt cgggctcgag
     7981 gtcctcgggg ttgacgtggg cgaggtcgtt gcggccgagg taggtgacgg cgagctcgcg
     8041 gaagatgtag tcgatgatcg aggtcgacat cttgatccgc gggtgcccct gcaccgagcc
     8101 gttcggctcg aagcgggtga acacgtaggc gtcgacgaac tcctcgagcg gcacgccgta
     8161 ctgcaggccg aggctgatcg cgatggcgaa gcagttcatg aggctgcgga aggctgcgcc
     8221 ctccttgtgc atgccgatga agatctcacc gagggtgccg tcgctgtagt cgccggtgtg
     8281 gatgtagacc ttgtggccgc cgatcgtcgc cttctgggtg tagccggcgc ggcggtcggg
     8341 catgcggcgg cgcttggcga ggtagcggtg gacgatgcgc tcggtgatct tctcgatcac
     8401 ctgcggctgg ctggtcgaga gggtctgctc ggtcccgtcc tcggcctcga cgacgaggcc
     8461 ggagctcagc ggctggctca gcttgctgcc gtcgcggtac agcgcgttgg ccttgacgcc
     8521 gtacttccac gacgcgaggt agacgtcgcc gacctcggtg acggtcgcgt cgctcggcat
     8581 gttgatcgtc ttcgaaattg cgccggacag gaacggctgg gcggccgcca tcatgcgcac
     8641 gtgcgccatc ggggcgatga agcgccggcc gtagcggccg cagcggctcg cgcagtcgaa
     8701 caccgggagg tgctggctct tgaggtgcgg ggcgccctcg acggtcatcg tgccgcacac
     8761 gtagtcgttg gcgcgctgga tgtcctcgac cgagaagccg agcgcctcga gcacgctaaa
     8821 cgccgggttg ccgagctggg cgtcggtgaa gccgagcacg ccgtgacaaa agtcgtcgcc
     8881 gaggacgtag cggttgaaca cgaagccgat ctcgaaggcg ccggcgagct cgctctcgac
     8941 gcgctgcagc acctcgtcgg tgaagccctt gcgcttgagg tcggcgtggg agatccgcgg
     9001 gctgccgacg agcgtgccgg cgcccttgca gtagcgcacg atgtcggtga tctgcgtgtc
     9061 ggtgtagccg agggtcgcca gcgcggccgg gacgcactga ttgatgatct tgaagtagcc
     9121 gccgccggcg agcttcttga acttgaccag cgcgaagtcc ggctcgatgc cggtcgtgtc
     9181 gcagtccatc agcaggccga tcgtgccggt cggagcgatg acggtcacct gcgcgttgcg
     9241 gtagccgtgc tgctcaccga gctgcagcgc cgtgtcccac tcctgctggg cggccttgag
     9301 caggtacggg gggcacatgc gctggtcgat gccgcgcggc gcggtcgaca ggccctcgta
     9361 ctcgctgcgg gcgacgtcgt aggcagcgcg gcgatggttg cggatcacgc ggagcatcgt
     9421 ctcgcggttg ggcg
//
LOCUS       DASWNJ010001749         1965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_596657, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001749 DASWNJ010000000
VERSION     DASWNJ010001749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596657"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VGB85_29200"
     CDS             complement(<1..705)
                     /locus_tag="VGB85_29200"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEY0138210.1"
                     /translation="MPTQAPPDSDLGFPAKGSLKRVPFAYLVRDIARTRTTGNLYLLA
                     GETKKVVFFVDGRPTLVRSNLASEFLGQILTDQGLITQEQCDNTLEAIRRTGKKQGEL
                     LVEMGILSEGNLRYGLEEQVRIKLYEIFGWDEGRFQFKQSGEPGMPATGMSLEIPVEA
                     LILAAVQERWNDERARAQLKTVADRFPVVHPRWTGDLTALDLLPEEQYFVRCIDGSRS
                     VDELLARKYDPEVPQVS"
     gene            1057..1785
                     /locus_tag="VGB85_29205"
     CDS             1057..1785
                     /locus_tag="VGB85_29205"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEY0138211.1"
                     /translation="MVSALVAVVLAAGKGTRMGSDLPKVLHVLHGEPLVAHPIRAARA
                     AGAGTVVVVVGHGADQVQAALGAGDDLRYALQSEQRGTGHAVLCALPAIAGHEGDVLI
                     LSGDVPRLRAGTLQRLAEACRASPGGLAFATFTPIDPTGYGRVVRDGQGQVLAIVEER
                     DASPEQRLILECNAGVYCASAALLRSELPQLGSSNAQGEIYLTDLVAVAAGQGVTALT
                     VPAEEVAGVNTREQLAALAALPPV"
BASE COUNT          245 a          666 c          767 g          287 t
ORIGIN      
        1 cgacacctgc gggacctcgg ggtcgtactt gcgggcgagc agctcgtcga cgctgcggct
       61 gccgtcgatg cagcgcacga agtactgctc ctcgggcagc aggtcgagcg ccgtgaggtc
      121 gccggtccag cgcgggtgca cgacggggaa gcggtcggcg acggtcttga gctgggcccg
      181 cgcgcgctcg tcgttccagc gctcctggac cgcggcgagg atcagcgcct cgacagggat
      241 ctcgagcgac atgccggtcg ccggcatgcc gggctcgccg ctctgcttga actggaaccg
      301 gccctcgtcc cagccgaaga tctcgtagag cttgatgcgg acctgctcct cgaggccgta
      361 acgcaggttg ccctcggaca ggatccccat ctcgacgagc agctcgccct gcttcttgcc
      421 agtgcggcgg atcgcctcga gggtgttgtc gcactgctcc tgcgtgatga ggccctggtc
      481 ggtcaggatc tggccgagga actccgacgc gaggttggag cgcaccagcg tcggccggcc
      541 gtcgacgaag aacacgacct tcttggtctc gccggcgagc aagtacaggt tgccggtcgt
      601 gcgcgtgcgg gcgatgtcgc ggacgaggta cgcgaagggc acgcgcttga gggatccctt
      661 ggccgggaag ccgaggtcgc tgtcgggggg tgcttgcgtg ggcatgatgg ggcgatgcga
      721 cggtggcagc ggagcgacgt ggaccccggg cggtgcaagg cgaggctccc caggataccg
      781 cggtccgcgg gctccggggc ccctgcctcg cgcccgtgtc gcaaccccgc gtcggaccgg
      841 tcgtcccggg cgcgcaggcg ccacgcccgg ggagccggcc cggggccgtg agagcccgcc
      901 cggaccgcac cgcggcggct gcgcggcggc gcgcggcgag gccgtcgtcg cggcgcagat
      961 ctgctcgcgg ttcgccgtcg ggcgcctcga ccgggacggg ccgtggcggt cggcggcgaa
     1021 gtggctggct cttgggtcag gtggtagggt gcgcgcatgg tcagcgcgct ggtcgccgtc
     1081 gtcctcgctg cgggcaaggg gacccgcatg ggctccgatc ttccgaaggt cctgcacgtg
     1141 ctgcacggcg agccgctggt cgcccacccg atccgcgcgg cccgggcggc cggggccggg
     1201 accgtggtcg tggtcgtcgg ccacggcgcg gaccaggtgc aggcggcgct gggggccggg
     1261 gacgacctgc gctacgcgtt gcaatccgag cagcgaggca ccgggcacgc ggtgctgtgc
     1321 gccctgccgg cgatcgcagg gcacgagggc gatgtcctga tcctcagcgg cgacgtgccg
     1381 cggctgcgcg ccgggaccct gcagcggctg gccgaggcgt gccgcgcgag ccccggtggc
     1441 ctggccttcg ccaccttcac gccgatcgac cccaccggct acgggcgggt cgtgcgagac
     1501 ggccagggcc aggtgctggc gatcgtcgag gagcgcgacg cgagcccgga gcagcggctg
     1561 atcctcgagt gtaacgcggg cgtgtactgc gcgtcggctg cgctgctgcg cagcgagctc
     1621 ccgcagctcg gcagcagcaa cgcgcaggga gagatctatc tcaccgatct cgtcgccgtc
     1681 gcggcggggc agggggtgac ggcgctgacg gtgccggccg aggaggtcgc cggcgtcaat
     1741 acccgcgagc agctggccgc gctggccgcc ttgccgccgg tatagtcagc cgttttcagc
     1801 tcgccgaagt ttcacgccag gccggcgacc tcgtcgacct ggcgcttgag gtcgtggatg
     1861 tcgaagggct tctcgatgaa gcggtcgcag ccggcctcca tcgcctgggt ccgctcgcgc
     1921 ttcatgacgc tggcggtcac ggcgagcacc gggatccggg cgatc
//
LOCUS       DASWNJ010001750         1842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_490528, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001750 DASWNJ010000000
VERSION     DASWNJ010001750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490528"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /gene="leuD"
                     /locus_tag="VGB85_29210"
     CDS             complement(<1..473)
                     /gene="leuD"
                     /locus_tag="VGB85_29210"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEY0138212.1"
                     /translation="MQPFTTLTGVAAPLPLINVDTDKIIPARWLKTIKRTGLGVAAFE
                     TVRYLPDGSENPAFVLNREPYRNAQILIAGDNFGCGSSREHAPWALLDFGIRCVIAPD
                     YADIFFNNSFKNGILPIVLPQEQVDLLTKEAENAPDPTFTIDLEAMEIRRPTGNQP"
     gene            664..1623
                     /locus_tag="VGB85_29215"
     CDS             664..1623
                     /locus_tag="VGB85_29215"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEY0138213.1"
                     /translation="MKRRHVAAVVTAALLAGAAWGGQALAQEKKTLALVTNASADFWT
                     IARRGTEKAAAELPNYNVEMHVVSEATAAEQRRILDDLLTKGVAGVSISSIDSANSTD
                     ILNKVAKQAVLFTTDSDAPKSERVLYIGTDNTAAGVQAGELIKQSLPNGGKIMMFVGT
                     MGADNARERVEGIKKSLAGSKVEIIDIRTDEVDFARAKRNAEDTLTKYPDVAALVGLY
                     SYNTPQIVEAVRASGRMGQVKIIGFDEDPVTLRGIADGIIDATVVQQPYEFGYQSMKL
                     LAKVIEGDKSFIPKDGLMIIPTKVIDKSNVAEFQTTMKNLLKK"
BASE COUNT          319 a          584 c          674 g          265 t
ORIGIN      
        1 ggctggttgc cggtcgggcg gcggatctcc atggcctcca ggtcgatggt gaaggtcgga
       61 tctggcgcgt tctccgcctc cttggtcagc aggtcgacct gctcctgggg gaggacgatg
      121 ggcaggatgc cgttcttgaa gctgttgttg aagaagatgt cggcgtagtc aggcgcgatg
      181 acgcagcgga tgccgaagtc gagcagcgcc cagggcgcgt gctcgcggga gctgccgcag
      241 ccgaagttgt cgccggcgat caggatctgg gcgttccggt agggctcgcg gttgaggacg
      301 aaggccgggt tctccgagcc gtcgggcagg tagcgcacgg tctcgaacgc ggccacgccc
      361 aggccggtgc gcttgatggt cttgagccag cgcgccggga tgatcttgtc ggtgtcgacg
      421 ttgatcagcg gcagcggggc ggcgacgccg gtcagggtgg tgaagggctg catgggggct
      481 tcctcgaggg cgcgggccgg gtgcggcggg ccgggtcggc cgcgggcgga tctgttacgc
      541 caagcgccgt cgcgcgacaa tcgcaggctg gggggtaggc gcgacgcggc gacgcggcta
      601 ggttcggcgc gcccatgcag ccgggggcga ggacgccccg ccggccgggt cagggagcta
      661 tcaatgaaga gacgtcatgt cgccgccgtc gtgacggcag cgctgctcgc gggtgcggcg
      721 tggggcggtc aggccctcgc gcaggagaag aagacgctgg cgctggtgac caacgcgtcg
      781 gccgacttct ggacgatcgc gcgccgcggc accgagaagg cggccgccga gctgccgaac
      841 tacaacgtcg agatgcacgt cgtctccgag gccaccgcgg ccgagcagcg ccgcatcctc
      901 gacgacctgc tcaccaaggg cgtggccggc gtctcgatca gctcgatcga ctcggccaac
      961 tcgaccgaca tcctgaacaa ggtcgccaag caggcggtgc tgttcacgac cgacagcgac
     1021 gcgcccaaga gcgagcgcgt gctctacatc ggcaccgaca acacggcggc cggcgtgcag
     1081 gccggcgagc tgatcaagca gtcgctgccg aacggcggca agatcatgat gttcgtcggc
     1141 accatgggcg ccgacaacgc ccgcgagcgg gtcgagggca tcaagaagtc gctggccggc
     1201 agcaaggtcg agatcatcga catccgcacc gacgaggtcg acttcgcccg cgccaagcgc
     1261 aacgccgagg acacgctgac caagtacccc gacgtggcgg cgctggtcgg gctctactcc
     1321 tacaacacgc cgcagatcgt cgaggcggtg cgcgcctcgg gccggatggg ccaggtcaag
     1381 atcatcggct tcgacgagga cccggtgacg ctgcgcggca tcgccgacgg catcatcgac
     1441 gccaccgtgg tgcagcagcc ctacgagttc ggctaccagt cgatgaagct gctggccaag
     1501 gtcatcgagg gcgacaaatc gttcatcccg aaggacgggc tgatgatcat cccgaccaag
     1561 gtgatcgata agtcgaacgt ggccgagttc cagacgacga tgaagaacct gctcaagaag
     1621 taggtcggat cgagcgcagc gctgatccga cgcggctccg acgcggcggc cgaaccgagg
     1681 caccgtgtcg gctccgcgct gcgcttgagc cgacctgcaa cagccgcctt ttcgggcggg
     1741 agagcggcga gccatgcacc agcccttcct cgaactgcgc gacatcacca agacctaccc
     1801 gggcgtgaca gcactgggcg gggtgagcct cgccgtgtcg tc
//
LOCUS       DASWNJ010001751         7232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_490578, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001751 DASWNJ010000000
VERSION     DASWNJ010001751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7232
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490578"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(278..757)
                     /locus_tag="VGB85_29220"
     CDS             complement(278..757)
                     /locus_tag="VGB85_29220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138214.1"
                     /translation="MPVLAHLSAGTAALTLALAPAAPRPLPESEIPGGYYEAMDLRRD
                     DEPPDGDKKITLGSVLFALGLIQAGGGVSSYITATPRYCPSVYGANVSDTTCSGLRIY
                     GIVGIVFGGLMATTGATMLGWGLIQRKKHREWMRERGLTLGPMLGPGHHGVSLGFRF"
     gene            complement(757..1947)
                     /locus_tag="VGB85_29225"
     CDS             complement(757..1947)
                     /locus_tag="VGB85_29225"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0138215.1"
                     /translation="MHRLTSLIALALLAACSQPPAAPGQPAGAPVPAASSGAPGPKDS
                     PGVQADGEVVSAVQWFEGSFEEAQAAAKASGKRLFVDVGAYWCPPCRKLDEEVFTQKE
                     VGEALGRDYVALHIDAEKGEGPDFVARYRVQAYPTLLVLEAGGLEQGRLVDAMAPAEL
                     LAALGEIARGGNVLATLEAAVQAEPDDLEARYRLGNAYALAAKRAEAEAELAKVIAGD
                     PDNARGLAAKAMADRAGFLVAKQDGDPERAIALYRELQQKFPTAKESLRAYRQIGREL
                     HKLGRDDEAVASLEAMVAAEPGNVDLKSAFGWFSFRERCRPEAGLKAVDAGIALDPQN
                     ADLHYVRAELRHQLGDGAGALDSIREAAAIEPESAFYKRQVRRFTELASEQAAVRPAA
                     MGGA"
     gene            2118..4736
                     /gene="clpB"
                     /locus_tag="VGB85_29230"
     CDS             2118..4736
                     /gene="clpB"
                     /locus_tag="VGB85_29230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEY0138216.1"
                     /translation="MAVFNPETATVKTRHAIAHAQAMTKDLGHPELTSLHLLMAALQQ
                     EGGLALPLLERTGVHGAAIDRAVKAELARQPRVQGAEPVPAKELRDLLDLAAGEAEAL
                     HDRFVSTEHLILACFSAKASKLKIRAAEVLGKLGGSRELLLSALAEIRGTQTVQDDSP
                     EEKYEVLAKYCRDLTQAAREQKLDPVIGRDVEIRRAIQVLSRRTKNNPVLIGDPGVGK
                     TAIAEGIALRIAVGDVPESLKDRKLMQLDLAALVAGAKYRGEFEERLKAVLKEVAAAD
                     GKIILFIDELHTLVGAGKAEGAQDAANMLKPALARGELRCIGATTLDEFRKHIEKDKA
                     LERRFQPVMIDEPSVEDTISILRGIRDKFEAHHGIRIMDAAIVAAARLSHRYIQNRFL
                     PDKAIDLVDEASARLKMEVESLPLPIDERERRITRLEIERQALLREDRTDATKNRLTQ
                     VDAELAALREEVTALRSRWQSERDRIDEIKQLTAQIENIKAEAARAQQAADYARAGEL
                     IHGTLRALEQQREQARKAIEQARQSQDSFLREAVTDEDIAGVVSRWTGIPVSKMLETE
                     QTRLLEMEANIGKRVVGQSEAIARVCASVRQSRAGLGDPNRPIGSFLFLGPTGVGKTE
                     LAKALAEFLFDDERHVIRVDMSEYMEKFSVSRLIGSPPGYVGHEEGGQLTEAVRRRPY
                     SVVLLDEIEKAHPEVFNLLLQVLDDGRLTDSQGHVVDFKNTIILMTSNIGSDLLSQDI
                     RGKQLEAARDQILRKHFRPEFLNRLDGILNFHPLRREDMMQILDIQLDRVRQRLAGRE
                     MELEVTPTAKALLAERGYDPAFGARPLKRLVQQTLLDPLATEILSGKLRPGSKVVADL
                     GQDSDGEPQVTLRPAA"
     gene            4839..5696
                     /locus_tag="VGB85_29235"
     CDS             4839..5696
                     /locus_tag="VGB85_29235"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0138217.1"
                     /translation="MRWFAASLAVISAGCYRDPGVLECDSPEAPEHCVSASAIATSSE
                     TGTSTQHEPDTSTGVGSTSSSTGPDDSSESGTTSALDTGTSGAASFCGDGTLDESEEC
                     DDGNLTADDGCNVTCKRDRIVFVSSEPGFLGGMLQGLKGADNYCVSRAAQAGFLNFLK
                     FKAFLSDSKTDAVDRLFAGEGRYVLVDGTVVADDWQALLTKPLQHPIELTELGEVLHR
                     SVWTGTRYGDGRAVPGSTHCDGWTSEDPDKQASFGFSDEVDVNWTQSQFFNPTSCGSM
                     LPIYCIEQR"
     gene            6009..6149
                     /locus_tag="VGB85_29240"
     CDS             6009..6149
                     /locus_tag="VGB85_29240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138218.1"
                     /translation="MLDPGEACDHGIYNWDHGACTSQCQAATCGDGLLFEGVEACDSE
                     LF"
     gene            6188..7054
                     /locus_tag="VGB85_29245"
     CDS             6188..7054
                     /locus_tag="VGB85_29245"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0138219.1"
                     /translation="MRWLAASLAVISAGCYRDLGVLECDSPSPPGHCVSAASETGPSS
                     ETSSSTSTQGQPDTSTGVASTSSSTGPDDSSESGTTSALDTGTSGAAPFCGDGTLDDD
                     EECDDGNLVADDGCNALCKRDRQVFVSSEPGFFGGMLQGLKGADNYCVSRAAQAGFAH
                     FLKFKAFLSDSKTDAVDRLFAGEGRYVLVDGTVVADNWQALLTEPLQHPIELTELGEV
                     LHRAVWTGTRYGDGRAVPGSTHCDGWTSEDPDEQASFGISDEVDVNWTQSQYFNPGSC
                     GSMYAIYCIEQQ"
BASE COUNT         1203 a         2641 c         2412 g          976 t
ORIGIN      
        1 gaaaatgact cggccagacg acgggggcaa gcccaggcgc cccccactat agccgcggcc
       61 acgaaaggat gtcagcaggt tcctgcgggc agatctgcgc cgatcgcccc ggcgacgtcc
      121 tcccgggagc cccccggcgg acacttgacc gcggtcttcc ccggatttcg cgcgcgacac
      181 cggacttcct gcgcgccgcc ctccaccccg ggtccgggcc ccgtacggaa gttcgctcgc
      241 acctgtccga tcggtcaggt acggcggcgc gccggcacta gaagcgaaag ccgagtgaca
      301 cgccgtgatg gcccgggccg agcatcgggc cgagggtcag gcctcgctcg cgcatccact
      361 cgcgatgctt cttgcgctgg atgaggcccc agccgagcat cgtggcgccg gtggtcgcca
      421 tcagcccgcc gaacacgatg ccgacgatgc cgtagatccg caggcccgag caggtcgtgt
      481 cggagacgtt ggcgccgtag accgaggggc agtagcgcgg cgtcgcggtg atgtagctgc
      541 tgaccccgcc gccggcctga atgaggccca gcgcgaacag gaccgacccc agggtgatct
      601 tcttgtcgcc gtcgggcggc tcgtcgtccc ggcgcaggtc catcgcctcg tagtagccgc
      661 cggggatctc cgactccggc agcggccggg gcgccgcagg ggccagcgcg agcgtgagcg
      721 cggctgtccc cgcagacagg tgagcgagga ccggcatcac gcacccccca tcgccgcggg
      781 ccggacggcc gcctgctccg aggcgagctc ggtgaagcgg cggacctgcc gcttgtagaa
      841 ggccgactcc ggctcgatcg ccgccgcctc gcggatgctg tcgagcgcgc cggccccgtc
      901 gccgagctgg tgacgcagct cggcgcgcac gtaatgaaga tcggcgtttt gcgggtcgag
      961 ggcgatcccg gcgtcgaccg ccttcagtcc ggcctccggc cggcagcgct cgcggaacga
     1021 gaaccacccg aacgccgact tgaggtcgac gttgccgggc tcggccgcga ccatcgcctc
     1081 gaggctggcg accgcctcgt cgtcgcgccc aagcttgtgc agctcacggc cgatctgccg
     1141 gtaggcccgc aggctctcct tcgcggtcgg gaatttttgt tgcagctcgc ggtacagggc
     1201 gatcgcccgc tcggggtcgc cgtcctgctt ggcgaccagg aaccccgccc ggtccgccat
     1261 cgccttggcc gccagcccgc gcgcgttgtc cgggtcaccg gcgatcacct tcgccagctc
     1321 ggcctcggcc tcggcccgct tcgccgccag cgcgtaggcg ttgccgaggc ggtagcgcgc
     1381 ctcgaggtcg tccggctcgg cctgcacggc cgcctcgagc gtcgccagca cgttcccccc
     1441 acgagcgatc tccccgagcg cggcgagcag ctcggctggc gccatcgcgt cgaccagccg
     1501 gccctgctcc agaccgcccg cctcgagcac gagcaaggtc gggtacgcct gcacccggta
     1561 gcgcgcgacg aagtccggac cctcgccctt ctccgcgtcg atgtgcagcg ccacgtagtc
     1621 gcggccgagc gcctcgccga cctccttctg cgtgaacacc tcctcgtcga gcttgcggca
     1681 cggcggacac cagtaggcgc cgacatcgac gaacagccgc ttgccggacg ccttcgccgc
     1741 ggcctgggcc tcctcgaagc tgccctcgaa ccactgcacc gccgacacga cctcgccgtc
     1801 ggcctggacc ccggggctgt ccttggggcc aggtgcgccg gagctggccg cagggacagg
     1861 cgcgcccgcc ggctgacccg gggcggcggg gggttgcgag caggcggcca ggagcgcgag
     1921 ggcgatcagg gacgtgaggc gatgcacggc gaacacactg cccgctcccg gggggtcacg
     1981 caaccgacca cggcccggtg cgcacctgcg aagccgccgc ggccgccgcc gcggccgttc
     2041 acggtgcttt ggccgctgcg cttgccacca gaaagatcgt gcacaagctg cccgaagccc
     2101 gaacagggag cacccccatg gccgtcttca accccgaaac cgcgaccgtc aagacccgtc
     2161 acgccatcgc tcacgcgcag gcgatgacca aggacctcgg ccaccccgag ctcacgagcc
     2221 tgcacctgct catggcggcg ctgcagcagg agggcggcct ggcgctgccc ctgctcgagc
     2281 gcaccggcgt gcacggtgcg gcgatcgatc gcgccgtcaa ggccgagctg gcgcgccagc
     2341 cccgggtgca aggcgccgag ccggtccccg cgaaggagct gcgcgacctg ctcgacctcg
     2401 ccgccggcga ggccgaggcg ctgcacgacc gctttgtcag caccgagcac ctgatcctcg
     2461 cctgcttcag cgccaaggcc agcaagctca agatccgcgc cgccgaggtg ctcggcaagc
     2521 tcggcggcag ccgtgagctg ctgctgtccg cgctcgccga gatccgcggc acccagaccg
     2581 tgcaggacga ctctcccgag gagaagtacg aggtgctcgc caagtactgt cgcgacctga
     2641 cccaggccgc ccgcgagcag aagctcgacc cggtgatcgg ccgcgacgtc gagatccgcc
     2701 gcgcgatcca ggtgctgtcg cggcgcacca agaacaaccc cgtcctgatc ggcgaccccg
     2761 gcgtcggcaa gaccgcgatc gccgagggca tcgccctgcg catcgcggtc ggcgacgtgc
     2821 ccgagagcct caaggaccgc aagctgatgc agctcgacct cgcggcgctc gtcgccggcg
     2881 ccaagtaccg cggcgagttc gaggagcgcc tcaaggccgt gctcaaggag gtcgccgccg
     2941 ccgacggcaa gatcatcctg ttcatcgacg agctgcacac gctcgtcggc gccggcaagg
     3001 ccgagggcgc ccaggacgcc gccaacatgc tcaagccggc gctcgcgcgc ggcgagctgc
     3061 gctgcatcgg cgcgacgacc ctcgacgagt tccgcaagca catcgaaaag gacaaggcgc
     3121 tcgagcgccg gttccagccg gtgatgatcg acgagccgag cgtcgaggac acgatctcga
     3181 tcctgcgtgg catccgcgac aagttcgagg cgcaccacgg catccgcatc atggacgccg
     3241 cgatcgtcgc cgccgcgcgc ctgagccacc gctacatcca gaaccgcttc ctgcccgaca
     3301 aggcgatcga cctcgtcgac gaggcctccg cgcgcctcaa gatggaggtc gagagcctgc
     3361 cgctgccgat cgacgagcgc gagcgccgga tcacccgcct ggagatcgag cgccaggcgc
     3421 tgctgcgcga ggaccggacc gacgcgacca agaaccggct cacgcaggtc gacgccgagc
     3481 tcgccgcgct gcgcgaggag gtcaccgccc tgcgctcgcg ctggcagtcc gagcgcgacc
     3541 gcatcgacga gatcaagcag ctgaccgccc agatcgagaa catcaaggcc gaggcggccc
     3601 gcgcccagca ggccgcggac tacgcccgcg ccggcgagct gatccacggg accctgcgcg
     3661 cgctcgagca gcagcgcgag caggcccgca aggcgatcga gcaggcccgc cagagccagg
     3721 actcgttcct gcgcgaggcc gtcaccgacg aggacatcgc cggcgtcgtg tcgcgctgga
     3781 ccggcatccc ggtcagtaaa atgctggaga ccgagcagac ccgcctgctc gagatggagg
     3841 cgaacatcgg caagcgcgtc gtcggtcaaa gcgaggcgat cgcccgcgtg tgcgcctcgg
     3901 tgcgccagtc ccgggccggc ctcggcgacc cgaatcggcc gatcggcagc tttttgttcc
     3961 tcggaccgac cggcgtcggc aagaccgagc tcgccaaggc gctcgccgag ttcctgttcg
     4021 acgacgagcg ccacgtcatc cgcgtcgaca tgagcgaata catggagaag ttctccgtgt
     4081 cgcgcctgat cggctcgccg cccggctacg tcggccacga ggagggcggc cagctgacgg
     4141 aggccgtgcg ccgccggccc tacagcgtcg tgttgctcga cgagatcgag aaggcccacc
     4201 cggaggtctt caatctattg ctgcaggtgc tcgacgacgg ccgcctgacc gacagccagg
     4261 gccacgtggt cgacttcaaa aatacgatca tcctgatgac cagcaacatc ggcagcgacc
     4321 tgctcagcca ggacattcgc ggcaagcaac tcgaggccgc gcgcgaccag atcttacgaa
     4381 agcacttccg gccggagttc ttgaaccgcc tcgacggcat tctgaacttc cacccgctgc
     4441 gccgcgagga catgatgcag atcctcgaca tccagctcga ccgcgtgcgc cagcgcctcg
     4501 ccggccgcga gatggagctc gaggtcaccc cgaccgccaa ggcgctgctc gccgagcgcg
     4561 gctacgaccc cgccttcggc gcccgcccgc tcaagcgcct ggtccagcag accctgctcg
     4621 acccgctcgc caccgagatc ctgtccggca agctgcgccc cggtagcaag gtcgtcgccg
     4681 acctcggcca ggacagcgac ggcgaacccc aggtcaccct gcgccccgcg gcgtgatgac
     4741 aaatacgggg aaacgccggt cgccggggtc tggaaggccc cggcgaccgg cttttaaagc
     4801 gtttgacggg cgatgcgcga tttcgctatc ctgccggggt gcgatggttt gcagcgtccc
     4861 tggcggtgat ctcggccggt tgttaccgcg accccggcgt gctcgagtgc gactcgccag
     4921 aggcgcccga acattgcgtg agcgcctcgg cgatcgcgac gtccagcgag accggcacga
     4981 gcacgcagca tgaaccggac acgagcaccg gcgtgggctc gaccagctcg agcacgggcc
     5041 ccgacgacag ctcggagagc ggaacgacga gcgccctcga caccgggacc agcggcgccg
     5101 cgtccttctg cggcgacggg accctcgacg agtccgagga gtgcgacgac ggcaatctca
     5161 ccgcggacga cggctgcaac gtgacgtgca agcgcgacag gatcgtgttc gtcagcagcg
     5221 agcccgggtt cctcggcggg atgctccagg gcctcaaggg cgcggacaat tactgcgtgt
     5281 cgcgggcggc ccaggccggt ttcctgaact tcctcaagtt caaggcgttc ctgagcgact
     5341 cgaagaccga cgccgtcgac cgcctgttcg ccggcgaggg ccgctatgtc ctggtcgacg
     5401 gcaccgtcgt cgccgacgac tggcaggcgc tgctgactaa accgttgcaa cacccgatcg
     5461 agctgaccga gctcggcgag gtcttgcatc ggtccgtgtg gacagggaca cgctacggcg
     5521 acggccgggc cgtgcccggg tcgacgcact gcgacggctg gacctcggaa gaccccgaca
     5581 agcaagccag cttcggcttc agcgatgagg tcgacgtcaa ctggacgcag tcgcaattct
     5641 tcaaccccac ctcgtgcggc tcgatgcttc ccatctactg catcgaacaa agatgaaagg
     5701 ccaggatgcc catgacccct catgatgaga cccaccgatc ttttcctcgc cctggacgac
     5761 tcgtccggtt catgacctct cctgttcgcc gcgtgcagcg gccgccgccc cggacgggcc
     5821 cgtaccggag gatctaccgg catgacgagc accggcacga cgatcaccga tgagcccccg
     5881 ggcgcgtcaa gcaccggtga agaagtgcca acgactggac cggcttcgac cgatgacctc
     5941 ggctcgacaa gcgatgccac tgacgcgacc acgagcggga caacaggcgt gccgacctgc
     6001 ggcacgccgt gctcgacccc ggcgaggcct gcgatcacgg catatacaac tgggatcacg
     6061 gcgcctgcac ctcccagtgc caggccgcga cctgcggcga cggcctgctg ttcgagggcg
     6121 tcgaggcgtg cgatagcgag ctattttagc gttgacgagc gatgcgcgat ttcgttaccg
     6181 tgcccgggtg cgatggcttg cagcgtccct ggcggtgatc tcggccggtt gttaccgcga
     6241 cctcggcgtg ctcgagtgcg actcgccgag cccgcccggc cactgcgtga gcgcggcgtc
     6301 ggagaccggg ccgtccagcg agacgagctc gagcacgagc acgcaagggc aaccggacac
     6361 gagcaccggc gtggcctcga ccagctcgag cacgggcccc gatgacagct cggagagcgg
     6421 aacgacgagc gcgctcgaca ccgggaccag cggcgccgcg cccttctgcg gcgacgggac
     6481 cctcgacgat gacgaggagt gcgacgacgg caacctcgtc gccgacgacg gctgcaacgc
     6541 gctttgcaag cgtgaccggc aggtgttcgt cagcagcgag cccgggttct tcggcgggat
     6601 gctccagggc ctcaagggcg ccgacaacta ctgcgtgtcg cgtgccgccc aggcgggctt
     6661 cgcgcatttc ctcaaattca aggcgttcct gagcgactcg aagaccgacg ccgtcgaccg
     6721 cctgttcgcc ggcgaaggcc gctacgtgct cgtggacggc accgtcgtcg ccgacaactg
     6781 gcaggccctg ctgaccgaac cgttgcaaca cccgatcgag ctgaccgagc tcggcgaggt
     6841 cttgcatcgg gccgtctgga ccgggacacg gtacggggac ggccgggccg tgcccgggtc
     6901 gacgcactgc gacggctgga cctcggaaga ccccgacgag caggccagct tcggcatcag
     6961 cgacgaggtc gacgtcaact ggacacagtc gcagtacttc aaccccggct cctgcgggag
     7021 catgtacgcc atctactgta tcgaacaaca atgaaaggcc aggatgccca tgacccctca
     7081 cgatgagacc gaccgatctt tgcctcgccc cggacgactc gtccggttga tgacctctct
     7141 cctgttcgcc gcgtgcagcg aggccgctac cccggccggg cccgtaccgg agggatcgac
     7201 cggaacgacg agcaccggca cgacgctcgt cg
//
LOCUS       DASWNJ010001752        12111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_279219, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001752 DASWNJ010000000
VERSION     DASWNJ010001752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 12111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12111
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279219"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1811)
                     /locus_tag="VGB85_29250"
     CDS             complement(<1..1811)
                     /locus_tag="VGB85_29250"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971064.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEY0138220.1"
                     /translation="MKLSRLFTQAQGRAYAGIDFERRSSRITNTDGTTVFEAADIEVP
                     QGWSQVAVDIMAQKYFRKAGVPARLRRVPEEGVPEWLWRSEPDALELAKLPANERSVG
                     EADSRQLFHRLAGCWTYSGWKYGYFADEESARVFYDELVTMLAAQMVAPNSPQWFNTG
                     LYWAYGITGPAQGHSYCDEKTGELKWASSAYERPQPHACFIQSVSDDLVGEGGIMDLW
                     VREARLFKYGSGTGTNFSALRGEGEPLSGGGRSSGLMSFLRIGDRAAGAIKSGGTTRR
                     AAKMVVVDVDHPDVERFIDWKVIEEQKVAALVAGSKLCNRHLNLVIGACHSGDPTDDG
                     RFDPRKNKELASAIRAARASEIPESYVQRAVALARQGARGIEFEEYDTGWDSAAYGTV
                     SGQNSNNSVRVTDDFLRAVEEDGTWDLIRRTDGKVHRTVRARELWDKIAMSAWSCADP
                     GLQFDTTINEWHTCPTDGRINASNPCSEYMFLDDTACNLASLNLSKFMVGSGPNAGDF
                     DVDAVRYACRVWTVVLEISVLMAQFPSTEIAERSYKFRTLGLGYANLGTYFMLRGIPY
                     DSDEAVAICGGITAIMTAASYAASAELAREHGPFPGYA"
     gene            2520..3050
                     /locus_tag="VGB85_29255"
     CDS             2520..3050
                     /locus_tag="VGB85_29255"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY0138221.1"
                     /translation="MTRDANAIFRAENGALERLSALETLKGLKNREAVTLGDLVRYLE
                     SRGLWAQFGKITLADLRDGFGAPPEPSANDEAAAAAKPAKKKKQRILEDELADIEVSP
                     TAKEAPPEDGGMSTDEVARQVLPFVEGNGDVTVEDIAEYTRIDRKVLRHHLAALVKQG
                     RLERIGVGRHAVYSTL"
     gene            complement(3076..5619)
                     /locus_tag="VGB85_29260"
     CDS             complement(3076..5619)
                     /locus_tag="VGB85_29260"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY0138222.1"
                     /translation="MPPAPHPRLRDRWIAWVLRFHRLVLVLAVVVTALAGWQASRLHI
                     DSDLRSLLPAGDPVLVSLDRIETSFGALGSVNIVVKDGTPEQRRAFADELAARIAESP
                     LVRDVEYRLRSEFFADHALYYFSDAEMEQLAELVSAWTHYELCKSEPDVCLEPPDAAA
                     PDALQKFVQDMQRKASGRTSFRDYFEREGIAALVLLVHPTRASSDLDFSQRVTAEMRA
                     TVAEVHAKPDAAWAGSAMRWNIVGPYIVKADERATISEDMLKSGLFGFFGVLAILYLL
                     LRSGRAVTTLMAPLVCGVVWSMAFTQLVLGHLNTLTSLISTVVMGMGIDAGIHFLLRV
                     REEQAGVSDSEAITRAFGGLIVPLLVASGTTLGAFAVMASSAFPAFHEFGLIAGSGVA
                     LCLLAMVTVYPAALRLTGVKRISRRAEWDEVAAPSASLLRGMRRALQRRPEAADLLSM
                     AVLRRPGLVLVIVVLLSGVAGYGARRVHFENNGRELQSDRTRAQAESDIFLISEIFGR
                     DIHAGILVVDTLEQARAVLASGRADHAARLAAGGSSTVAEMFGAADLLPDPAIDLAKR
                     KQAIAAITEDVPEATWERLEKRAADREAKAEGLSPEEARRLQRMLKAEPVTLGRLPPA
                     LLDKLRSGDGRHAVYAYPNFDAADIGKGVEFMAETATYTQGLAAQSDASEPVFVGETT
                     VYALMYRMMLREAPYVLGASAALIALLVLLQLRSIGQTLRTLLPLALGMLWLVGLMGL
                     LDVRFTLFNIPILPAILGIGVDNGVYLTDRIRRLHGEPGGLASALGGTGAAILAATAT
                     TVMGFAAFMIADNGGLRGIGALAVMGISIAALTAILVLPSLAALSRPRR"
     gene            complement(5693..6583)
                     /locus_tag="VGB85_29265"
     CDS             complement(5693..6583)
                     /locus_tag="VGB85_29265"
                     /inference="COORDINATES: protein motif:HMM:NF016218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEY0138223.1"
                     /translation="MSSVRPANKTMVYPNKTPELTRRRLLLAAAGATTAALLPRTSQA
                     LTAVAKPTTPVDPNAKKILILGDSMIAGAVGLFLENGLRKEHGYNVHRKGKSSTGLAR
                     PDFFSWPKEAQAQVEAFPPDAVVAMFGGNDVQGLYMGKGEWITWPEPGWSAEYARRIN
                     AFADIVAPGGKPLFWIGMPVMRPEKFHVRVQRVNTIFRAEMAIRPGGHFIDIWRLLAN
                     DRGEYADRLDIDGVPGGKTERVRAGDGIHLSVAGAHRVEAHVRAIIHQVLSGTSADPV
                     GSPTVAAGDPGAPTSVPGGA"
     gene            6670..7017
                     /gene="grxD"
                     /locus_tag="VGB85_29270"
     CDS             6670..7017
                     /gene="grxD"
                     /locus_tag="VGB85_29270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEY0138224.1"
                     /translation="MTDLDTQKYLANLVSNHKVLLFMKGNKTFPQCGFSAAVVEVLKR
                     HGVPFETINVLQDASVRDGIKQFSSWPTIPQLYINGEFIGGCDIVREMDANGELADKL
                     QAAKNPQETPREA"
     gene            complement(7048..7737)
                     /locus_tag="VGB85_29275"
     CDS             complement(7048..7737)
                     /locus_tag="VGB85_29275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138225.1"
                     /translation="MRALTCFTALFTLVALPACSDDGGGTTNPSTQSSTGDLTATMTD
                     ATAGTMTDATAGTMDAPTTTGMTGMTTPTTMNDDGSAGTTTDGSAGTTTDGSAGTTTD
                     GSAGTTTDGSAGTTTDTGDTDTGGGGSDLYGPCEMGGTPCPGDQECLKLGSVEGYFCA
                     PACVGMKQCPEVPEGVTAQAMCVLKKPMQDVASNCALLCQPDMDGQCPAGTTCKDVPM
                     QDPPIGVCTSP"
     gene            complement(7915..8667)
                     /locus_tag="VGB85_29280"
     CDS             complement(7915..8667)
                     /locus_tag="VGB85_29280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138226.1"
                     /translation="MRALTSFTTTLTLILGLAACGDDKETTTQTTQTPATDPTATATD
                     PSTGTTADDGTTTDTPTTNTPTTGDDASAGTTTNVGTTSTTDVGTTTDVGTTTDTTVS
                     TTEDVGTTTDAPDTTGDVDTTTGGAADPNWPVPDPMNAMMMLCPEGFFGAQFSEGGAV
                     CAPKCDAQNMCPAGATGDAQAGCLFNPTSSGMVCKMGDKCPGMNEMCVQTGGGGMACL
                     APPDHCVLLCDGGETCPDGMECFDDLICQYPV"
     gene            8866..9600
                     /locus_tag="VGB85_29285"
     CDS             8866..9600
                     /locus_tag="VGB85_29285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138227.1"
                     /translation="MAHADFKVFIAGGLLGVGLIACAPTESAGECTASKPCTNRGEAC
                     DEVVQECVPQDLAVDATDDEPTPASFSNVALPFFRGRVCMPLTVQPGDTVPVKISPCL
                     HPCIDPGGFNFKKQYTCKGSYCDAAVVQVFGAAAGDGCPADAFGKFDKSQCMYADDIK
                     ASAGPFVIEGAPVRGNAQVEVPFLTNDDAARIRDGASVADIWELIKQYPQSQERVFAI
                     SMDGANPAAPADCSDESKCDCRDIGF"
     gene            9672..10856
                     /locus_tag="VGB85_29290"
     CDS             9672..10856
                     /locus_tag="VGB85_29290"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY0138228.1"
                     /translation="MLSGGGARGAYEVGVLRYIRERLKCEARIDIITGSSIGAINGAY
                     IAATADRPRAQGRLLARVWSELNLDEVCRFGWSQMRALPQMLFGKVRDKTTHGAQLGG
                     LVDPRAMQALVRTRLPWHAITENLHRGHLSAISVTATEVATGNVTIFVQTASGTLPPH
                     WPSAQRQSVVLTPITAAHALASGAIPVMFPAVRVGDQLYSDGGLRQNTPLRPAMRLGA
                     TRLLVIGLRHKPDEGELGQIREEAESTFPSAMFMMGKVLNVLLLDKVENDLERIQRMN
                     QILEAGTREFGPDFSARLASAMHKDKSTPYSHVHTLLIRPSQDLGRVAFETVQRTGLS
                     NYSGVVARWIRWAVQADGARQESDLASYLLFDREYVKRLIDLGYADAARHHDELVALF
                     NE"
     gene            complement(10860..11237)
                     /locus_tag="VGB85_29295"
     CDS             complement(10860..11237)
                     /locus_tag="VGB85_29295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138229.1"
                     /translation="MVAPTDEGVYGEQRTSALPAKDRTQAPGQVKFEREGCLTDPAKC
                     KCQSKDATKAALCSSMLGHFCPDSTLRYLAAACMDLFGGVTLCQCAEQKQIENLVKAE
                     DAAKAKADKKAAAKKKPKAVTAK"
     gene            11459..>12111
                     /locus_tag="VGB85_29300"
     CDS             11459..>12111
                     /locus_tag="VGB85_29300"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEY0138230.1"
                     /translation="MSADSSTTEALQARVSACFADRSLLSDPATREAVLQTIALLDRG
                     ALRVATPGEPGAGWTVHAWVKQAILLFFATQQMRTMELGPFEFHDKIALKRDWAGAGV
                     RVVPPATARYGSFIESGAVLMPSYVNIGAWVGGGTMVDTWATVGSCAQIGRHVHLSGG
                     VGIGGVLEPPGAAPVIVEDGCFLGSRAIVVEGVHLEREVVLGANVTLTASTPIIDVTG
                     "
BASE COUNT         1814 a         4233 c         4257 g         1807 t
ORIGIN      
        1 gcgtagccgg ggaagggccc gtgctcgcgc gccagctcgg cgctcgcggc gtaggaggcg
       61 gcggtcatga tcgccgtgat gccgccgcag atcgccacgg cctcgtcgct gtcgtacggg
      121 atcccgcgca gcatgaagta ggtgccgagg ttggcgtagc cgaggccgag ggtgcgaaac
      181 ttgtagctgc gctcggcgat ctcggtgctc gggaactggg ccatcaggac cgagatctcg
      241 agcacgaccg tccacacgcg gcaggcgtag cgcaccgcgt cgacgtcgaa gtcacccgcg
      301 ttcggaccgc tgccgaccat gaacttgctc aggttgagcg aggccaggtt gcaggcggtg
      361 tcgtcgagga acatgtactc ggagcagggg ttgctcgcgt tgatccggcc gtcggtcggg
      421 caggtgtgcc actcgttgat cgtggtgtcg aactgcaggc ccgggtcggc gcacgaccac
      481 gcgctcatgg cgatcttgtc ccagagttcg cgagcgcgga cggtgcggtg caccttgccg
      541 tcggtgcgac ggatcaggtc ccaggtgccg tcctcctcga ccgcgcgcag gaagtcgtcg
      601 gtgacgcgca ccgagttgtt gctgttctgc ccggagacgg tcccgtaggc ggccgagtcc
      661 cagccggtgt cgtactcctc gaactcgatg ccgcgcgcgc cctgacgcgc gagcgccacg
      721 gccctttgca cgtagctctc ggggatctcg gacgcgcggg cggcccggat cgccgaggcg
      781 agctccttgt tcttgcgcgg gtcgaagcgg ccgtcgtcgg tcgggtcgcc ggagtggcag
      841 gcgccgatca cgaggttgag gtggcggttg cagagtttgc ttccggcgac cagcgcggcg
      901 accttttgct cctcgatcac cttccagtcg atgaagcgct cgacgtccgg gtggtcgacg
      961 tcgacgacga ccatcttggc ggcgcgccgg gtggtcccgc cgctcttgat cgcgccggcg
     1021 gcccggtcac cgatgcgcag gaagctcatc aggccgctcg agcgaccgcc acccgagagc
     1081 ggctcgccct cgccgcgcag cgccgagaag ttggtgcctg tgcccgagcc gtacttgaac
     1141 agccgcgcct cgcggaccca caggtccatg atgccaccct cgccgacgag gtcgtcgctg
     1201 accgactgga tgaagcaggc gtgcggctgc gggcgctcgt aggcgctgct cgcccacttg
     1261 agctcgccgg tcttctcgtc gcagtagctg tgcccctgcg ccggcccggt gatgccgtag
     1321 gcccagtaca ggccggtgtt gaaccactgc gggctgttcg gcgcgaccat ctgcgcggcg
     1381 agcatcgtca ccagctcgtc gtagaacacc cgcgcggact cctcgtcggc gaagtagccg
     1441 tacttccagc ccgagtaggt ccagcaaccg gcgaggcggt ggaacagctg gcggctgtcg
     1501 gcctcgccga cgctgcgctc gttggccggg agcttggcca gctcgagcgc gtcgggctcg
     1561 ctgcgccaca gccactcggg cacgccctcc tcgggcaccc gccgcagccg cgccggcacg
     1621 ccggccttgc ggaagtactt ctgcgccatg atgtcgaccg ccacctgcga ccagccctgc
     1681 ggcacctcga tgtcggcggc ctcgaagacc gtcgttccat cggtgttggt gatgcgcgaa
     1741 ctgcgtcgct caaagtcgat gccggcatag gcgcgacctt gggcctgggt gaacagccga
     1801 gacagcttca tgggtacgga ctccggggaa cgaggggggg caggaggtgg gcggaggtgg
     1861 gcgcgcgcga acggacgagg atccgaaccg ctccggcccc tcggctgccg gggggaggca
     1921 gcggaggagt cggccggtcg gcgcgcaaat tgtcgaagcg ataagcgccc gcgggcagga
     1981 cgaacgaggc gaaaaacgca agccgaaacg acccgcgagg ccgcgcgcgg agcaaccccg
     2041 cgcacggcga cgaccccaca tctgggcgtc gggcgaccta taaaccacaa gatgttgggg
     2101 tcgtgcaagt ttgcgaaccg cgcgcttgcg gtcgaccccg gcccccgggc cgcgggcaat
     2161 agcgcgagtt gttcacagct tccccacctg tctctcggtt ccggaaggga ttgtggaatg
     2221 ccgtttcatc ggccctccga gatcgctcag cggggggcca gcgcgcgcct gcgggactcc
     2281 cgccccacag cgctcgaagc gctgcacagg ggcctggcgc gaccgaagtc gcccattcgc
     2341 tggatacata ttatccggtg ccaggggggc gcgaatcgcg gtgcacgggg ccccgggaga
     2401 tctctccgcg ggcatggatt tcaccgttcg actccgccca atgtgaccac gatccggggc
     2461 tcaatgggtg ggtcccggct tgcgcggatc gcggccatcc gccatatcaa gacagttcga
     2521 tgacaagaga tgccaacgca attttccggg ccgaaaacgg cgcgctcgag cggctgagcg
     2581 cgctcgagac gctcaagggc ctcaagaacc gcgaggccgt gaccctcggc gacctcgtcc
     2641 gctacctcga gtcgcggggc ctgtgggccc agttcggcaa gatcaccctc gccgacctgc
     2701 gcgatggctt cggcgccccg cccgagccga gcgccaacga cgaggccgcg gccgcggcca
     2761 agccggcgaa gaagaagaag cagcggatcc tcgaggacga gctcgccgac atcgaggtct
     2821 cgccgaccgc caaggaagct ccgcccgagg acggcggcat gtcgaccgac gaggtcgccc
     2881 gccaggtcct ccccttcgtc gagggcaacg gcgatgtcac cgtcgaggac atcgccgagt
     2941 acacccgcat cgaccgcaag gtgctccggc accacctcgc cgcgctggtc aagcagggcc
     3001 ggctcgagcg gatcggtgtc ggccgacacg cggtctactc gacgctctag gccgctcgcg
     3061 gagcgcggcc gaggctcacc ggcgcggccg cgacagcgcg gcgaggctcg gcagcacgag
     3121 gatcgccgtc agcgcggcga tcgagatccc catcacggcg agggcaccga tgccacgcag
     3181 gccgccgttg tcggcgatca tgaacgcagc gaagcccatg acggtggtcg cggtcgccgc
     3241 caaaattgca gcgccggtgc ccccgagcgc gctggcgagg ccgccgggct cgccatgtag
     3301 acggcggatg cggtcggtca ggtagacgcc gttgtcgacc ccgatgccga ggatcgccgg
     3361 caagatcggg atgttgaaca gggtgaagcg gacgtcgagc aggcccatca ggccgacgag
     3421 ccagagcatg ccgagcgcga gcggcagcag ggtccgcagg gtctggccga tcgaccgcag
     3481 ctgcaacaag accagcaagg cgatcagcgc ggccgaggcc ccgagcacgt acggggcctc
     3541 gcgcagcatc atgcggtaca tcagcgcgta gacggtcgtc tcgccgacga acaccggctc
     3601 ggaggcgtcg ctctgggcgg cgaggccctg cgtgtaggtc gcggtctcgg ccatgaactc
     3661 gacgcccttg ccgatgtcgg cggcgtcgaa gttcgggtag gcgtacacgg cgtggcggcc
     3721 gtcgccgctg cgcagcttgt cgagcaacgc gggcggcagg cgccccaggg tcaccggctc
     3781 ggccttgagc atgcgctgca gccggcgcgc ctcctcgggg ctgaggccct cggccttcgc
     3841 ctcgcggtcc gcggcgcgct tttcaaggcg ctcccaggtg gcctcgggga catcctcggt
     3901 gatcgccgcg atcgcctgct tgcgcttcgc caggtcgatc gcgggatcgg gcaagaggtc
     3961 agccgcgccg aacatttcgg cgaccgtgct cgagccgccg gcggcgaggc gcgcggcgtg
     4021 gtcggcccgg ccgctggcga gcacggcgcg cgcctgctcg agcgtgtcga cgacgaggat
     4081 ccccgcgtgg atgtcccgcc cgaagatctc gctgatgagg aagatgtcgg actcggcctg
     4141 cgcgcgcgtg cggtccgact gcagctcgcg gccgttgttc tcgaagtgga cccgccgcgc
     4201 gccgtagccg gcgacgccgc tgagcagcac cacgatcacc agcaccaggc cgggccggcg
     4261 cagcaccgcc atgctcaaca ggtccgccgc ctcggggcga cgctgcagcg cgcgtcgcat
     4321 gccccgcagc agcgaggccg agggcgccgc gacctcgtcc cactccgccc gccgggagat
     4381 ccgcttgacg cccgtcaggc gcagcgccgc ggggtagacg gtgaccatcg ccagcaggca
     4441 cagcgcgacg ccggagccgg cgatcaggcc gaactcatgg aacgcgggga acgccgagct
     4501 ggccatcacc gcgaacgcgc cgagcgtcgt gccggaggcg acgagcaagg gcacgatcag
     4561 cccgccgaac gcgcgcgtga tcgcctcgct gtcggacacg ccggcctgct cctcgcgcac
     4621 gcgcagcagg aagtgaatgc cggcgtcgat gcccatgccc atgacgaccg tcgagatcag
     4681 gctggtcagg gtgttgaggt ggccgagcac cagctgcgtg aacgccatcg accacacgac
     4741 gccgcacacc agcggggcca tcagggtggt cacggcgcga cccgagcgca gcagcaggta
     4801 gaggatcgcc aggacgccga agaagccgaa caggccgctc ttgagcatgt cctcgctgat
     4861 cgtcgcgcgc tcgtcggcct tgacgatgta cggcccgacg atgttccagc gcatcgccga
     4921 gccggcccac gcggcgtccg gcttcgcgtg cacctcggcg accgtcgccc gcatctcggc
     4981 ggtcacccgc tgcgaaaaat cgaggtcgct cgaggcgcgc gtcggatgca ccagcaacac
     5041 cagcgccgcg atgccctcgc gctcgaagta gtcgcgaaag ctcgtccgcc cggaggcctt
     5101 gcgctgcatg tcctgcacga acttttgcag ggcgtccggg gccgcggcgt cgggcggctc
     5161 gaggcacacg tcgggctcgg acttgcacag ctcgtagtgc gtccacgcgg acaccagctc
     5221 ggcgagctgc tccatctcgg cgtcgctgaa gtaatagagc gcgtggtcgg cgaagaactc
     5281 gctgcgcagg cgatactcga cgtcgcggac gagcgggctc tcggcgatgc gcgcggcgag
     5341 ctcgtcggcg aaggcgcggc gctgctcggg ggtgccgtcc ttgacgacga tgttgacgct
     5401 accgagcgcc ccgaacgacg tttcgatgcg gtcgaggctg acgagcacgg ggtcgccggc
     5461 gggcagcagc gagcgcaggt cgctgtcgat gtgcaggcgg gaggcttgcc agccggcgag
     5521 cgccgtgacg acgacggcga gcacgagcac gagccggtga aagcgcagga cccaggcgat
     5581 ccagcgatcg cgcaggcgcg ggtggggggc cggcggcaag ggcggccatg gtgccccaag
     5641 cgcctccatg gaaccagcgc ccgaccgggc cgatcgcgcg aaaccgatcg gctcacgccc
     5701 cgccgggcac gctcgtcgga gctccgggat cgccggccgc gacggtcggg ctgccgaccg
     5761 ggtccgcgga ggtgcccgag agcacctgat ggatgatcgc ccggacgtgg gcctcgaccc
     5821 gatgcgcgcc cgccacgctg aggtggatcc cgtcgcccgc gcgcacgcgt tcggtcttgc
     5881 cgccgggcac gccgtcgatg tcgaggcggt cggcatactc gccgcgatcg ttggccagca
     5941 ggcgccagat gtcgatgaag tgcccgccgg ggcggatcgc catctcggcg cggaagatcg
     6001 tgttcacgcg ttgcacccgc acgtgaaact tctcgggccg catcaccggc atgccgatcc
     6061 aaaacagcgg cttgccgccg ggcgcgacga tgtcggcgaa ggcgttgatc cggcgggcgt
     6121 actccgccga ccagccgggc tcgggccacg tgatccactc gcccttgccc atgtagaggc
     6181 cctggacgtc gttgccgccg aacatcgcga ccaccgcgtc gggcgggaac gcctcgacct
     6241 gcgcctgcgc ctccttgggc cacgagaaga agtccggccg cgcgaggccg gtgctgctct
     6301 tgcccttgcg gtgcacgttg tagccgtgct ccttgcgcag cccgttctcg aggaacaggc
     6361 cgaccgcgcc ggcgatcatg ctgtccccga ggatgaggat cttcttcgcg ttcgggtcga
     6421 ccggggtggt cggcttcgcc actgccgtca gcgcctggct cgtgcgcggc agcagcgcgg
     6481 ccgtcgtcgc gcctgccgcg gccagcaaca ggcggcggcg cgtcagctcg ggggtcttgt
     6541 tcgggtacac catggtcttg ttcgccgggc ggacggacga caatgtgccc aaacttgttc
     6601 ggccgcgcac tggttttcac gcaccggcgc gggtatcacc atcggcccgg ccccccggga
     6661 gacctcgcca tgaccgacct cgacacgcaa aagtacctcg ccaacctcgt cagcaaccac
     6721 aaggtcctgc tgttcatgaa gggcaacaag accttcccgc agtgcgggtt ctcggccgcc
     6781 gtcgtcgagg tgctcaagcg ccacggcgtg cccttcgaga cgatcaacgt gctgcaggac
     6841 gccagcgtgc gcgacggcat caagcagttc tccagctggc cgacgatccc ccagctctac
     6901 atcaacggcg agttcatcgg cggctgtgac atcgtccgcg agatggacgc caacggcgag
     6961 ctcgcggaca agctgcaagc tgcaaagaac ccgcaagaga ccccgcgcga ggcctgagcg
     7021 gctcgcgggg gccacggggt ccgacgctca cggagaagtg cagacgccga tcggcgggtc
     7081 ctgcatcggg acatccttgc aggtcgtgcc ggcggggcac tgcccgtcca tgtccggctg
     7141 gcacagcagc gcgcagttgc tggcgacgtc ctgcatcggc ttcttgagca cgcacatcgc
     7201 ctgcgcggtg acgccttccg gcacctccgg gcactgcttc atgccgacgc aggcgggcgc
     7261 gcagaagtaa ccctcgacgc tgccgagctt gaggcactcc tggtccccgg ggcacggcgt
     7321 gccgcccatc tcgcacgggc cgtagaggtc gctgccacca ccaccggtgt ctgtgtcgcc
     7381 cgtgtcggtc gtcgtcccgg cgctgccgtc ggtcgtcgtc ccggcgctgc cgtcggtcgt
     7441 cgtcccggcg ctaccgtcgg tcgtcgtccc ggcgctgccg tcggtcgtcg tcccggcgct
     7501 accgtcgtcg ttcatggtcg tcggggtcgt catgcccgtc atgcccgtcg tggtcggcgc
     7561 gtccatcgtg ccggcggtcg cgtcggtcat cgtgccggcg gtcgcgtcgg tcatcgtcgc
     7621 ggtgaggtcg ccggtcgagc tctgggtcga cggattggtg gtgccgccgc cgtcgtcgct
     7681 acacgccggg agggcgacga gggtgaacag ggccgtgaaa caggtcagtg cgcgcatttg
     7741 taggctccgg agaatggtga tgcgcgcgtc cgggcgggtg ggccgcccgt gcgcgaaacg
     7801 ctacataatc gccgtgccca caaacgcacg cgtttcaccg aaccgcgaaa cgagacctct
     7861 cgcacgtgtc accgcggagg cggcgacacg tgcgtgccac tcgatacgag ccggtcagac
     7921 cgggtactgg cagatcaggt cgtcgaagca ctccatgccg tcggggcagg tctcgccgcc
     7981 gtcgcacagg agcacgcagt ggtccggcgg ggccaggcag gccatgccgc cgccgccggt
     8041 ctgcacgcac atctcgttca tgcccgggca cttgtctccc atcttgcaca ccatgcccga
     8101 cgaggtcggg ttgaagaggc acccggcctg cgcgtcgccg gtcgcgccgg cggggcacat
     8161 attttgggcg tcgcacttcg gcgcgcagac ggcgccgccc tccgagaatt gggccccgaa
     8221 gaagccctcc gggcacagca tcatcatcgc gttcatcggg tccggcaccg gccagttggg
     8281 gtcggcggcg ccaccggtgg tcgtgtcgac gtcgccggtg gtgtcgggag cgtccgtcgt
     8341 cgtgccgacg tcctccgtcg tgctcaccgt cgtgtcggtc gtcgtcccga cgtcggtcgt
     8401 cgtcccgaca tccgtggtcg acgtcgtccc gacgttcgtc gtcgtgccgg cgctcgcgtc
     8461 gtcaccggtc gtcggcgtgt tggtcgtcgg cgtgtcggtc gtcgtgccgt cgtccgccgt
     8521 cgtccccgtg ctcgggtcag tcgccgtcgc cgtcgggtcg gtcgccgggg tctgggtcgt
     8581 ctgagtggtg gtctctttgt cgtcgccgca ggctgcgagg ccgaggatga gggtgagcgt
     8641 ggtcgtgaaa gaggtcagtg cgcgcatttt caggtaactc cagagaaatt ggcgcgcgcc
     8701 ccgacgggga cgtcggcgcg cgcaaaacct agatacctgc cgcgcccacg aaggcacgta
     8761 tttgccgccg gcgcgaaagg agacgtcgcg cacatgattc ggcgcggaac cggcgacatc
     8821 cactcgtgcg tacgatacga tgccggacag acgaggaaaa tcacgatggc ccatgccgac
     8881 ttcaaggtgt tcatcgcagg tggtttgctc ggtgtcgggc tgatcgcctg tgccccgacc
     8941 gagagcgcgg gcgagtgtac cgccagcaag ccgtgcacca accgcggcga ggcgtgcgac
     9001 gaggtcgtgc aggagtgcgt gccgcaggac ctcgcggtcg acgcgaccga cgacgagccg
     9061 acgcccgcga gcttctccaa cgtcgcgctg ccgttcttcc gcggtcgcgt gtgcatgccg
     9121 ctgacggtcc agccgggcga caccgtgccg gtgaagatct cgccgtgcct gcacccgtgc
     9181 atcgacccgg gcggcttcaa cttcaagaag cagtacacct gcaaggggtc gtactgcgac
     9241 gcggcggtcg tgcaggtgtt cggcgcggcc gcgggcgacg gctgcccggc ggacgcgttc
     9301 ggcaagttcg acaagtcgca gtgcatgtac gccgacgaca tcaaggcgag cgccggcccg
     9361 ttcgtgatcg agggggcccc ggtccgcggc aacgcgcagg tcgaggtccc gttcctcacc
     9421 aacgacgacg ccgcgaggat ccgcgacggg gccagcgtcg cggacatctg ggagctgatc
     9481 aagcagtacc cgcaatcgca agagcgcgtg ttcgcgatct cgatggacgg cgccaacccg
     9541 gcggcgcccg ccgactgcag cgacgagagc aagtgcgatt gccgggacat cgggttctga
     9601 gcgagcacac gacagagcac gagcccgagt ccgagccgcc tccgcccggc acccgcccca
     9661 agatcggcct cgtgctgtcc gggggcgggg cccgtggcgc ctacgaggtc ggcgtcctgc
     9721 gttacatccg cgagcgcctc aagtgcgagg cccgcatcga catcatcacg ggtagcagca
     9781 tcggcgcgat caatggcgcc tacatcgctg ccaccgccga ccggccccgc gcgcagggcc
     9841 gcctgctcgc gcgcgtgtgg tccgagctca acctcgacga ggtgtgccgc ttcggctggt
     9901 cgcagatgcg ggcgctgccg cagatgctgt tcggcaaggt ccgcgacaag accacccacg
     9961 gcgcgcagct cggcggcctg gtcgacccgc gggcgatgca ggcgctggtg cgcacgcgcc
    10021 tgccgtggca cgcgatcacc gagaacctgc atcgcggtca cctctcggcg atctcggtga
    10081 cggcgaccga ggtcgcgacc ggcaacgtca cgatcttcgt gcagaccgcc agcggcacgt
    10141 tgccgccgca ctggccgtcg gcgcagcgtc agagcgtcgt gttgacgccg atcaccgccg
    10201 cccacgcgct cgcctccggt gcgatccccg tgatgttccc ggccgtgcgg gtcggcgacc
    10261 agctgtactc cgacggcggc ctgcgccaaa acaccccgct gcgcccggcg atgcggctcg
    10321 gcgcgacgcg cctgctcgtc attggtttac gtcacaagcc cgacgagggc gagctgggcc
    10381 agatccgcga ggaggccgag tcgaccttcc cgagcgcgat gttcatgatg ggcaaggtgc
    10441 tcaacgtgct gctgctcgac aaggtcgaga acgacctcga gcggatccag cggatgaacc
    10501 agatcctcga ggccggcacc cgcgagttcg gccccgactt ctccgcccgc ctcgccagcg
    10561 cgatgcacaa ggacaagtcg accccctact cgcacgtgca caccctgcta attcgtccga
    10621 gccaggacct cgggcgcgtc gccttcgaga ccgtccagcg caccggcctc agcaactact
    10681 ccggcgtcgt cgcgcggtgg atccgctggg ccgtgcaggc cgacggcgcc cgccaggaga
    10741 gcgacctcgc cagctacctc ctgttcgacc gcgagtacgt caagcgactg atcgacctcg
    10801 gctacgccga cgccgcccgc caccacgacg agctcgtcgc cctgttcaac gagtgatcgt
    10861 cacttggccg tgacggcctt gggcttcttc ttcgcggcgg ccttcttgtc ggccttggcc
    10921 ttggctgcgt cctcggcctt gacgaggttc tcgatctgct tctgctcggc gcactgacac
    10981 agggtcaccc cgccgaacag gtccatgcag gccgccgcga ggtagcgcag ggtgctgtcg
    11041 gggcagaagt ggccgagcat cgacgagcac agcgcggcct tggtcgcgtc cttgctctgg
    11101 cacttgcact tcgccgggtc ggtcaggcac ccctcgcgct cgaacttcac ctgccccggc
    11161 gcctgcgtcc tgtccttggc cggcagcgcg ctggtccgct gctcgccgta cacgccctcg
    11221 tcggtcggcg cgaccaacgg ctcgcctgat gcagcggatg cagcggatgc agcggatgcg
    11281 gcagatgtcc cgaaggccag ggacccgata gccagagaag cgacgaacag gcaggggatg
    11341 gcgcggcgaa cggacatgag gcctcgaacg atagcgcatc gccgggtccg gacgccagcg
    11401 tatccgcggc ggacgagcgc gcttgcgcga gcgcggccgg ccgtgcgatg cccggtgcat
    11461 gtccgccgac tcctccacga ccgaggcgct gcaggcccgc gtgagcgcct gtttcgccga
    11521 ccgcagcctc ctgtcggacc ccgccacccg cgaggccgtc ctgcagacga tcgccctgct
    11581 cgaccgggga gcgttgcgcg tcgcgacccc cggcgagccc ggcgctggct ggaccgtgca
    11641 cgcgtgggtc aagcaggcga tcctgctgtt cttcgccacg cagcagatgc gcacgatgga
    11701 gctcgggccg ttcgagttcc acgacaagat cgccctcaag cgcgactggg ccggcgctgg
    11761 cgtgcgcgtc gtgccgccgg ccaccgcccg ctacggcagc ttcatcgagt ccggcgccgt
    11821 gctgatgccg agctacgtca acatcggcgc gtgggtcggc ggcggcacga tggtcgacac
    11881 ctgggcgacc gtcggcagct gcgcgcagat cggtcgccac gtgcatctct ccggcggcgt
    11941 cggcatcggc ggcgtgctcg agccgccggg cgccgcgccc gtcatcgtcg aggacggctg
    12001 cttcctcggc tcgcgtgcga tcgtcgtcga gggcgtgcac ctcgagcgcg aggtggtcct
    12061 cggcgccaac gtcacgctga ccgcctcgac accgatcatc gacgtcaccg g
//
LOCUS       DASWNJ010001753        12495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_342528, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001753 DASWNJ010000000
VERSION     DASWNJ010001753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 12495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12495
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342528"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..2202
                     /locus_tag="VGB85_29305"
     CDS             190..2202
                     /locus_tag="VGB85_29305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEY0138231.1"
                     /translation="MSRTTLMFKVYHGETLVQSQRYDAEVVKLGSLPSSDLRIDDAAV
                     ARQHAVVEVVGDEVKLIDLGSAAGTLLNGQKISKFATLKDGDVMTLGNTRIQAQISNT
                     NAVAAPVAPATKVAPQIDAREFEVQNGSRVAQVTAMFNDTVIDVQHLGQVRDGRKTAP
                     AWLALGGCVALAGAALFGMEAAQDWDGYSERAVAAHEAGKPAPAKPGTGLGGLGFGLA
                     MLGLVPIGVGFTRLQDRGRRSYTLGESHDAAMHVPAATLPNGESFPLVQGDGDYALQF
                     TSDMQGELTVDGQSVTLAALISSGRATRVGAAYTVPLAPGAQARVRHGALTFFIHSVA
                     PGVVTARKSEIDKPFWVTNTSAFAVLGSLLVLTHLIPNEAGALSVQEDAENNRFVGYL
                     AQPNETIEEEEAVPNKPVQEVGGVAGTRHTGAEGKAGNPSSKKAEGRYAMVGPKDAIP
                     SLARDFNPEMAARNAGILGLMKADNSSFMASPFGGYAVGNDDQNFWGAVVGNEAAESY
                     GNGGLGLAGVGRGGGGTGQGTIGLGSVGTIGHNRPGDGSGIMRGAGFGDRTKRVPAPR
                     LGIAEVQGGLDKDLIRRVVRAHINEVRHCYNQGLARDPNLKGRVSVQFQIGATGKVPT
                     ANVSESDLKDKSVSNCIASAVRRWQFPKPQGGGTVIVTYPFVLQSE"
     gene            2298..4202
                     /locus_tag="VGB85_29310"
     CDS             2298..4202
                     /locus_tag="VGB85_29310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138232.1"
                     /translation="MPEPENTPRRGAFALCTLALLAMHLALFLYFVPPSAWWTDQPMT
                     GVDYETHIAQVWRVLEGLDGWGKTWVYDVRLLAGYPHGVLFDADNKGWEVWTWALRGL
                     GFSQGQAFNLFVVAAHWGVLAVTFTSARLFGLGRVGARAAAALASLLWFFDSFSHWCW
                     WVGMVAYGLASYLCLLPLALFYRWLQDRKTWQAVATAVTLGLVHLVHPYSFFILVAPM
                     LALYFKNVRGLGWRGQLAVVGIVATTVAFNAWWLAVALRFWHYILDSGFFGQSGLQFL
                     VADFFGLVLDTATSGMIGTRSGFRALFVAAAVLGLVLWRRQRDPRWLPFTAAIVTLAA
                     LAYLGAYAWIFRQIQPYRHVLPLMFFAVIPAAALLEATVRRGWLRGLGAGPKLAAGLL
                     ALPAAQHLISDVLYYAPSSLPKVAPLYNNGWLPITAQGFGPQPDYRHHAITEDYDVVV
                     DWVRANDDGQSRFLVEEPTLGEQLSWRTSAQLLGGFTYRNLQHSNANLFRRRKEGIAS
                     DAELRAYFETYAVEFLIITRPGKTWDRRTAVLEFVVQLGSHRVYRTKISPRLIAQGGG
                     KVEAQTNLIKVAGSDPGQDLILRFHWLETLVCEPDCAVGRAPIDEFDEVGFIKIAAPH
                     PADLEVRNAY"
     gene            4192..5061
                     /locus_tag="VGB85_29315"
     CDS             4192..5061
                     /locus_tag="VGB85_29315"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEY0138233.1"
                     /translation="MLIDDAARIALGVAVYRLRRLEMANIAGAAAICVALALPPAECV
                     LRLGFAFGLNVLVYLNNDYLDLATDLRAPGRDDAKTRFLRDHRAAGLTAQVGLLIVLL
                     GLCAFDPGLVVPLVLGGGVCWAYSARLKAMPYADVAAMVVWGVAMPLCGSPVDSALGW
                     ALAGWLGLFSGVFEAVQVIRDHEDDAAAGVRTTAVVLGVTRTFLLVRLMLAASAVYGA
                     LVVSPWLAVLPAAAMGIPLDRARVTRTWNHLRLVLGVAFLGAISWVVWFGATDGVLVQ
                     VARDMSLGTSTSP"
     gene            complement(5048..6388)
                     /locus_tag="VGB85_29320"
     CDS             complement(5048..6388)
                     /locus_tag="VGB85_29320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138234.1"
                     /translation="MRLHCVLLAASLCSCGDPDPGTGETGSSSQDPSTTSGSAASSSD
                     TTAEPTTAPTPTGTTAEPTTDTTAATTGELPDYSDSPCWGKPASTQVYNGQTHQISEV
                     AATCRAEGERTFLLVADALWDNGTIDQAAVNGFMHRYELFTPAGSIDPERGVIHNDEA
                     VFGTLDAAKLPGDKVSIFVIDSNGGGDGYLCGWCDYSQLHLDGTILTPLDGDNALSIS
                     AHESYHLIHHGHDADEEVWIDESLAQAAMTANGFFTDDEWLDDFRLAPDQDWGPGGVD
                     FGTFNYGAGLLWGTFLYERGGTALMTAITSEQANGWAGLDAALTLVGDDSDAWSLFLD
                     MGVAMVADRPDLGHGFTAFDVGEVALAGDLAAGEMAGGEVQPGGFDVYRLVGAGALEL
                     TVTGTGVGARAFRIAQDIVVVDPVGGATLELDADDDAYLLVSAQASAEYTITVK"
     gene            complement(6502..7575)
                     /locus_tag="VGB85_29325"
     CDS             complement(6502..7575)
                     /locus_tag="VGB85_29325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HEY0138235.1"
                     /translation="MSFRRLLPALLIAPLLATLAPRVADACSPPSGFTVERTFPAPDA
                     DDVGVDTNILVLGEGWADAQVSITVTQDGAPIEGHLSSNDASRYRWNPSDMLLPGATY
                     DVRVESVNQNLEMPLVVHEFSFTTAMALFPAPVPPTVKSAVVEVYEREIKECLVEDDG
                     GSCGGCLEWEVVDVEQRLRLVVDVEQPVGVYDGFYGSVVGWSVADPVPDAGDTQYQGP
                     EAGPTHHVIDLHKVGEYPGTQVCVDVHGENPVDLYIIVPEQPGCIDISELNIPLDGET
                     ETNTDSDDAPTTGGDESSDSSGDESSDSSGGESSGPGGDESDGPEQDGEAGCGCRSDA
                     APGGSLAMLALVLGVVRPRRRAR"
     gene            7810..7935
                     /locus_tag="VGB85_29330"
     CDS             7810..7935
                     /locus_tag="VGB85_29330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138236.1"
                     /translation="MLADLSRWPEIDPEALRFRLGNMLEAIRLARAVPAGGVRIS"
     gene            complement(7954..9099)
                     /locus_tag="VGB85_29335"
     CDS             complement(7954..9099)
                     /locus_tag="VGB85_29335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HEY0138237.1"
                     /translation="MLTGRSDVLIVGAGPAGAAAGTFLARMGLRVACVDRATFPRDKT
                     CGDAVSSAGIEIAASLGADLRTLPHAVVTAAAAILPDGTRIVRRYGDKPGAIVPRLAL
                     DDLLRQRMQAAGVTLHEGVAVRALLRDGARFTGAEGPALRWHARVILAADGPGSVAWT
                     ALGRPYPRGPQLGVATTVYLKNAAPAEPGVSEHYFERDLPCGYGWLFPPVDGLANAGV
                     YQRADYYDSGRTKLRALLHAFMARHPERFANAEITGPVRTWALPLSLHTWPLGGAPGL
                     LVCGDAGSFVDPLSGEGIWQALRSGQLAAEHAGAALTAGDQVDAAVVRRYRLACARAV
                     SWPSLVRAGIQESMRVLIDRGLDRHTIVRRALQWGYGTGRSELSRVE"
     gene            9172..11028
                     /locus_tag="VGB85_29340"
     CDS             9172..11028
                     /locus_tag="VGB85_29340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138238.1"
                     /translation="MSAPLRRVWLTLIGLHILLLAYALPIGVILGDSPLGGPDYQTHF
                     QHTHTLLQVQAEFGRAWAYDPNLLAGHPTGLIFDVDNKLHFGWTSMLVRLGVPLPTAF
                     NLFAWLACALAPVSLWLAARLLRMEVGARALAFGLGVLVWSFDPTARFCWAGGMISFA
                     FAAHVSTVVIAVMHRLLADGGRRLAVALLVLLPLVLRMHVWSFAILVVPLTGMYLRAW
                     RQIPARRHAVVWGAALLGLLVNLDWLLPALAHRDLIVPSARLGQATPPYLLFDYLELL
                     VDPLRTGFVMQRTLLRALVVIAAVGTLWSWRGDARRFTGGLTLAWLLGLTYAGALVPG
                     VQATEPYRFAVPLVLWATVLAGTWLLRAVEGLRGLQGAARGAVLGLLILVLPRLYHQL
                     MPYLTMLSPGPAPTGHAARSLPSARLEGVPADFEAVATWLEGQPRGGRVLVQYHPLGE
                     YLRWASDWPVLGGFYDRRMIFQDANLFYFAAGDDRYRDGLAAYLERYNVSHLVMTYPY
                     VAEVERRTDLFEPAGIQGGVHRVYTVRKRGSYFVEGVGEVQAGLNRIAVRAARPAPGS
                     QALTLRFHYMDELRCAPGCRVERAEVEGDTAGFVRVVGEPVLPAEFVVSLEY"
     gene            11057..11929
                     /locus_tag="VGB85_29345"
     CDS             11057..11929
                     /locus_tag="VGB85_29345"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEY0138239.1"
                     /translation="MAPVRYRLHQADCLAWMDAQPECSVHAIVTDPPYGLREYTAPET
                     AKLRSGKGGVWRIPPALDGCKRAPLPRFTVLSAADREALQAFFAAFAARVMRVLVPGG
                     HLFLAANPLLSTEIYVPLMAAGLEKRGEVIRLVQTLRGGDRPKNAHAEFSEVSVMPRS
                     GWEPWGLFRRPCEGRVQDNLRRWKTGGLRRISEDLPFSDVIASAPTQPAERAISPHPS
                     LKPQAFMRQIVRAALPLGEGVVLDPFMGGGSTIAAAVAVGYESIGVELDPAFFAVAEA
                     AIPRLAQLPVKLRG"
     gene            complement(11940..>12495)
                     /locus_tag="VGB85_29350"
     CDS             complement(11940..>12495)
                     /locus_tag="VGB85_29350"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY0138240.1"
                     /translation="ATLLARIAAFSQRPTPAEAQHLAATGDERALPALVAAMTDTMEG
                     QDVVRYRQAVRTLASAELLQRWLGGDLESRRVAAHALTAHDPAHAPLIGLALRDADDQ
                     VRAIARRSLRAWTASPALHDLFRETLANPDPRVRLLAAEGLGKLGKPADLPALTTALE
                     AETDSAARDRLAWAIERIEDDAEQ"
BASE COUNT         1683 a         4005 c         4725 g         2082 t
ORIGIN      
        1 gttcccgcga acgtgccagt ggagatcacg gcgcgggatt tttcaaaact tgccgctgac
       61 agcgagctac gggcgaggtt ccgcgtttta caagaatcgc ggcgatgaag aattaatgtg
      121 tcgccgcgga acctcgggcc cgggttcatg tctgtagggg ggtaaggctg acgtaaggag
      181 cccgctgcca tgtcccgcac gacgctgatg ttcaaggttt atcacggtga gacgctggtc
      241 cagagccagc gatacgacgc agaggtcgtg aagctcggca gcctgccgag cagcgacctc
      301 cggatcgatg acgccgcggt ggcccgccag cacgcagtcg tcgaggtggt cggcgatgag
      361 gtcaagctca tcgacctcgg aagtgccgcc ggcacgctcc tcaacggcca aaagatctcc
      421 aagttcgcaa cgctgaaaga cggtgatgtc atgaccctcg gtaacactcg catccaggcc
      481 cagatctcca acaccaacgc tgtcgccgcg cccgtcgctc ccgccaccaa ggtggccccg
      541 cagatcgacg ctcgcgagtt cgaggtccag aacggctcgc gcgtcgccca ggtgaccgcg
      601 atgttcaatg acacggtcat cgacgtgcag cacctcggtc aggtccgcga cggtcgcaag
      661 accgcgccgg cgtggctggc tctcggcggc tgcgtcgccc tcgctggtgc tgcgctgttc
      721 ggcatggagg ccgcgcagga ctgggacggt tacagcgagc gcgccgtcgc tgcgcacgag
      781 gctggcaagc ccgcgccggc caagccgggc accggtctcg gcggtctcgg cttcggtctg
      841 gcgatgctcg gcctggtgcc gatcggtgtc ggcttcacgc gtctgcagga ccgtggtcgt
      901 cgcagctaca cgctcggtga gagccacgac gcggcgatgc acgtgccggc ggcgacgctg
      961 cccaacggcg agtcgttccc gctggtccag ggtgacggcg actacgcgct gcagttcacc
     1021 tcggacatgc agggcgagct gacggtcgat ggtcagagcg tcacgctcgc cgcgctgatc
     1081 tcgagcggtc gtgcgacgcg ggtcggcgcc gcctacaccg tgccgctcgc ccccggggcg
     1141 caggcgcggg tgcgtcacgg cgcgctgacc ttcttcatcc actcggtcgc gccgggcgtc
     1201 gtgacggcgc gcaagagcga gatcgacaag ccgttctggg tcaccaacac cagcgccttc
     1261 gcggtgctcg gcagcctgct ggtgctgacc cacctgatcc cgaacgaggc gggcgcgctc
     1321 agtgtccagg aggacgccga gaacaaccgc ttcgtcggct acctcgccca gccgaacgag
     1381 acgatcgagg aggaggaggc ggtccccaac aagccggtgc aggaggtcgg tggcgtggcc
     1441 gggacgcggc acaccggcgc ggagggcaag gcgggcaacc cgtcgagcaa gaaggccgag
     1501 ggtcgctacg cgatggtggg cccgaaggac gcgatcccgt ccctggcccg cgatttcaac
     1561 ccggagatgg cggcgcgcaa cgccggcatc ctcgggctga tgaaggccga caacagcagc
     1621 ttcatggcct cgcccttcgg cggctacgcg gtcggcaacg acgatcagaa cttctggggg
     1681 gcggtcgtcg gcaacgaggc ggcggagtcg tacggcaacg gcggcctcgg tctcgcgggc
     1741 gtcggccgcg gcggtggcgg caccggccag ggcacgatcg gcctcggctc ggtcggcacg
     1801 atcggtcaca accggcccgg cgacggtagc gggatcatgc gcggcgccgg cttcggcgac
     1861 cgcaccaagc gggtccccgc gccccgcctc ggcatcgccg aggtccaggg cgggctcgac
     1921 aaggacctga tccgtcgggt ggtgcgcgcg cacatcaacg aggtccgtca ctgctacaac
     1981 cagggtctcg cgcgggaccc caacctgaag ggtcgcgtgt cggtgcagtt ccagatcggt
     2041 gcgaccggca aggtgccgac cgccaacgtg tccgagtccg acctcaagga caagagcgtc
     2101 tccaactgca tcgcgtcggc cgtccgtcgc tggcagttcc ccaagcccca gggcggcggc
     2161 acggtgatcg tcacctaccc cttcgtcctc cagtccgagt aaccactcgc tgactccccg
     2221 ccgaccgggc cgagctccaa gctcggcccg gtcgttttgc gtggttcatt gaccacgcgc
     2281 gcgtttatga tgcggccgtg ccggagcccg agaacacacc gcgtcgcggc gccttcgcgc
     2341 tatgcacgct ggcgctgctc gcgatgcacc tcgcgctgtt cctgtacttc gtgccgccct
     2401 ccgcgtggtg gaccgaccag ccgatgaccg gcgtcgacta cgagacccac atcgcgcaag
     2461 tgtggcgcgt gctcgagggc ctcgacggct gggggaagac gtgggtctac gacgtccgcc
     2521 tgctcgccgg ctatccacac ggggtgctgt tcgacgccga caacaagggc tgggaagtgt
     2581 ggacgtgggc gctgcgcggg ctcggcttct cgcaagggca agcgttcaat ctgttcgtgg
     2641 tggccgcgca ctggggcgtg ctcgcggtga ccttcacgtc ggcgcggctg ttcgggctcg
     2701 gaagagttgg cgcgcgcgcg gcagcagcgc tggcctcgct gctgtggttc ttcgactcgt
     2761 tctcgcactg gtgctggtgg gtcggtatgg tcgcgtacgg actggcgagc tacctgtgcc
     2821 tgctgccgct cgcgctgttc tatcggtggt tgcaagaccg caagacttgg caagcagtcg
     2881 ccacggcggt gacgctcggg ctggtgcacc tcgtgcatcc gtacagcttt tttatattgg
     2941 tcgcgccgat gctggcgctg tattttaaaa atgttcgcgg gctcggctgg cgtgggcagc
     3001 tggcggtcgt gggaattgtc gcgacgacgg tggcgttcaa cgcgtggtgg ctcgcggtgg
     3061 cgctgcgctt ttggcattac atcctcgact ccgggttctt cgggcagtcg gggctgcaat
     3121 ttttggtcgc ggacttcttc gggctcgtgc tcgacaccgc gacctcgggc atgatcggga
     3181 cgcggagcgg gtttcgggcg ctgttcgtcg ctgccgcggt gctcggcctg gtgctgtggc
     3241 ggcgccagcg tgacccgcgg tggctgccgt tcaccgccgc gatcgtgacc ctcgcggcgc
     3301 tggcctacct cggcgcctac gcgtggatct tccgccagat ccagccgtac cggcacgtgc
     3361 tgccgctgat gttcttcgcg gtcatcccgg cggcggcgct gctcgaggcg acggtgcgtc
     3421 gaggttggtt gcgcgggctc ggagcgggtc cgaagctcgc ggccgggttg ctggcgttgc
     3481 cggccgcgca gcacctgatc tccgatgttt tgtattatgc gccgagctcg ctgccaaagg
     3541 tcgcgccgct gtacaacaac gggtggctgc cgatcacggc ccagggcttc gggccccagc
     3601 ccgactatcg tcaccacgcg atcaccgagg actacgatgt cgtcgtcgac tgggtccgcg
     3661 ccaacgacga cgggcagtcg cggtttttgg tcgaagaacc aaccctcggc gagcagctca
     3721 gctggcggac cagcgcgcag ctgctgggtg gtttcacgta tcgcaacctg caacacagca
     3781 acgccaacct gtttcgccgg cgcaaggagg gcatcgccag cgacgccgag ctgcgggcct
     3841 acttcgagac ctacgcggtc gagtttttga taattaccag gccgggcaag acctgggacc
     3901 ggcgcaccgc ggtgctcgag ttcgtggtcc agctcgggtc gcaccgcgtc taccgcacga
     3961 agatctcgcc acggctgatc gcgcagggcg gcggcaaggt cgaggcccag acaaatttaa
     4021 taaaggtggc ggggagtgat ccggggcagg atctgatctt aagatttcac tggctggaga
     4081 cgctggtgtg tgagccggac tgcgcggttg gacgggcgcc gatcgacgag ttcgacgagg
     4141 tgggttttat taaaattgcg gcgccgcatc cggctgacct ggaggtgcgc aatgcttatt
     4201 gatgatgcgg cgaggatcgc cctcggggtc gcggtgtatc gcctgcgccg gctcgagatg
     4261 gccaacatcg ccggggcggc tgcgatctgc gtcgcgctcg cgctgccgcc cgcggagtgc
     4321 gtgctgcggc tcgggttcgc gttcgggctc aatgtgttgg tgtatttaaa taatgactac
     4381 ctcgacctgg cgaccgacct gcgcgcgcct ggtcgtgatg atgcgaagac tcgatttctg
     4441 cgcgaccatc gggcggccgg gctgacggcg caggtcggct tgttgatcgt gttgctcggc
     4501 ctgtgtgcgt tcgacccggg gctggtggtc ccgctggtgc tcggcggggg cgtgtgctgg
     4561 gcctactcgg cgcggctcaa ggcgatgccg tacgcggatg tcgcggcgat ggtcgtgtgg
     4621 ggcgtggcga tgccgctgtg cggcagcccg gtcgacagcg cgctcggatg ggcgctggcg
     4681 ggctggctcg ggctgttctc gggggtcttc gaagcagtac aggtgatccg cgatcacgag
     4741 gacgacgcgg ccgccggggt gcgcacgacg gcggtggtcc ttggggtcac gcgcacgttc
     4801 ttgctggtgc ggctgatgct cgcggcgagc gcggtgtatg gcgcgctggt ggtctcgccg
     4861 tggctcgcgg tgttgccggc ggccgcgatg ggcatcccgc tcgaccgcgc ccgggtgacg
     4921 cgcacgtgga accacctgcg cctggtcctc ggggtcgcgt tcctcggggc gatctcctgg
     4981 gtggtgtggt tcggggcgac cgacggcgtg ctggtgcagg tggcgaggga catgtccctc
     5041 gggacatcta cttcaccgtg atcgtgtatt cggcggaggc ctgcgcgctg accagcaggt
     5101 aggcgtcgtc gtcggcgtcg agctcgagcg tggcgccgcc gaccggatcg acgacgacga
     5161 tgtcctgcgc gatgcgaaag gctcgcgcgc cgacgccggt gccggtgacc gtgagctcga
     5221 gcgcgccggc gccgacgagg cggtagacgt cgaagccgcc gggctggacc tcgccgccgg
     5281 ccatctcgcc cgcggcgagg tcgcccgcga gcgcgacctc gccgacgtcg aaggcggtga
     5341 agccgtggcc gaggtcgggg cggtcggcga ccatggcgac gcccatgtcg aggaacagcg
     5401 accaggcgtc gctgtcgtcg ccgacgaggg tcagcgccgc gtcgaggccg gcccacccgt
     5461 tggcctgctc gctggtgatc gcggtcatca gggccgtacc gccgcgctcg tacaggaacg
     5521 tgccccacag caggccggca ccgtaattaa aagtgccgaa gtcgacgccg ccggggcccc
     5581 agtcctggtc gggcgcgagg cggaagtcgt cgagccactc gtcgtcggtg aagaagccgt
     5641 tggcggtcat ggcggcctgc gccaggctct cgtcgatcca cacctcctcg tcggcgtcgt
     5701 gaccgtggtg gatcaggtgg tagctctcgt gggcggagat cgacagcgca ttgtcgccgt
     5761 cgagcggggt gaggatggtg ccgtcgaggt ggagctgcga atagtcgcac cagccgcaca
     5821 gatagccgtc gccgccgccg ttgctgtcga tgacgaagat cgagaccttg tcgccgggca
     5881 gcttggcggc gtcgagcgtg ccgaacaccg cctcgtcatt gtggatgacg ccgcgctcgg
     5941 ggtcgatcga cccggcgggc gtgaacagct cgtaacgatg catgaagccg ttgacggccg
     6001 cctgatcgat cgtgccgttg tcccacagag catcggcgac cagcaaaaag gtgcgctcgc
     6061 cctcggcgcg acaggtggcc gcgacctcgg agatctggtg ggtctgaccg ttgtagacct
     6121 gcgtcgaggc gggcttgccc caacaggggg agtcgctgta gtcgggtagc tcgccagtgg
     6181 tcgcagcggt ggtgtcggtg gtgggctcgg cagtggtgcc ggtcggggtg ggcgcagtgg
     6241 tgggctcggc agtggtatcg ctcgagctcg ctgcgctgcc cgaggtcgtg gacgggtcct
     6301 gggagctgct gccagtctcg ccggtgcccg ggtcggggtc gccacagcta cacagagaag
     6361 ccgccagaag gacgcagtgg agtcgcatgc gcggcgagta taggccgggc cgggcgcgtg
     6421 ggtggtcagg ggcggcgttt cgcgggtgag caatgcgccg ccccgcgccc atgagctcct
     6481 tggaggcccg ggcaccgtgg atcaacgggc gcgtcggcgt ggacggacga cgccgagcac
     6541 gagcgccagc atagcgagcg agccgcccgg cgcggcatcg gagcggcagc cgcagccggc
     6601 ctcgccgtcc tgttcggggc cgtcggactc gtcaccgcct gggccgctcg actcgccacc
     6661 gctcgagtcg ctggactcgt cgccgcttga gtcgctggac tcgtcgccgc cggtggtcgg
     6721 ggcatcgtcg ctgtcggtgt tcgtttcggt ctctccatcg agggggatgt tgagctcgct
     6781 gatgtcgatg caacccggct gctccggcac gatgatgtac aggtcgaccg ggttctcacc
     6841 gtggacgtcc acgcacacct gggtccccgg gtattcgccg accttgtgga ggtcgatgac
     6901 atgatgggtc gggccggcct ccgggccctg atattgtgtg tcgcccgcgt cagggacggg
     6961 atccgccacg ctccacccga cgacgctgcc gtagaacccg tcgtagaccc cgacgggttg
     7021 ctcgacgtcg acgacgaggc gcaggcgctg ctcgacatcg acgacctccc actcgaggca
     7081 gcccccgcac gacccgccgt cgtcctcgac gaggcactct ttgatctcgc gctcgtacac
     7141 ctcgaccacg gcgctcttga ccgtcggcgg caccggcgcg gggaacagcg ccatcgcggt
     7201 ggtgaagctg aattcgtgga ccacgagcgg catctcgagg ttctggttca cgctctcgac
     7261 gcgcacgtca tacgtggcgc cggggagcag catgtcgctg gggttccagc gatagcggct
     7321 ggcgtcgttc gacgacagat ggccctcgat gggcgcgccg tcctgggtga ccgtgatgct
     7381 gacctgcgcg tccgcccagc cctcgccgag cacgaggatg ttggtgtcga ccccgacgtc
     7441 gtcggcatcg ggcgcgggga aggtgcgctc gaccgtgaat ccggacggcg gcgaacaggc
     7501 gtcggcgaca cgaggcgcga gggtggcgag cagaggggcg atgagcagtg cggggagcag
     7561 gcgacgaaaa gacatggcgg aggcctcgca gagcaagcgg cacgcccgtg ggcttctcgt
     7621 gtcatctcgg atatttggcc ggggccggcg gacccgtgtg atctcggtgg cccggagcgc
     7681 tggcgctgga cggtatacgg cgcgccggct gctcgtgcgc tcgggctgcg cctgctgccg
     7741 tcgctcacca ccggcgacct ccacgtcgaa ggagacgata tcgacgcgct cgagcgcgag
     7801 gtccacgaga tgctcgccga cctctcgcgc tggccggaga tcgatccaga ggcgctgcgc
     7861 tttcgtctcg gcaacatgct cgaggcgatc cgcctcgccc gcgccgtccc ggcgggcgga
     7921 gtgcgtatct cctgagggcg gtggaccgct tcgttactcg acgcggctca attcgctgcg
     7981 gccggtgccg tagccccatt gtaaagcgcg gcggacgatc gtgtggcgat cgaggccgcg
     8041 gtcgatcagg acgcgcatgc tctcctggat gccggcgcgg accagcgatg gccagctgac
     8101 cgcgcgcgcg caggccaggc ggtagcggcg gacgacggcg gcgtcgacct gatcgccggc
     8161 tgtcagcgcg gcgccggcgt gctcggctgc gagttgaccg ctgcgcagag cctgccagat
     8221 gccctcgccg ctgagcgggt cgacgaagct gccggcgtcg ccacatacga gtaatcccgg
     8281 ggcgccgccg agcggccagg tgtgcaggct cagcggcaag gcccaggtgc gcacggggcc
     8341 ggtaatctct gcattggcga agcgctccgg gtggcgggcc atgaatgcat gcaggagggc
     8401 gcgcagcttg gtccggccgc tatcatagta gtcggcgcgt tgatagacgc cggcgttggc
     8461 gaggccgtcg acgggcggga acagccagcc atagccgcag gggaggtcgc gctcgaagta
     8521 atgttcgctg acgccgggct cggccggggc ggcatttttt aaatagacgg tggtggcgac
     8581 gccgagctgc gggccgcggg ggtaggggcg gccgagggcc gtccaggcga ccgagccggg
     8641 gccgtcggcg gcgaggatga cacgggcgtg ccagcgcagc gcggggccct cggcgccggt
     8701 gaagcgggcc ccgtcgcgta gcagcgcgcg cacggcgacg ccctcgtgca acgtgacgcc
     8761 ggcggcttgc atgcgctggc gcaggaggtc gtcgagggcg aggcgtggga cgatcgcgcc
     8821 gggcttgtcg ccgtagcggc ggacgatccg ggtgccgtcc gggaggatcg cggcggcggc
     8881 cgtgacaacc gcgtggggga gggtgcggag gtcggcgccg aggctcgcgg cgatctcgat
     8941 gcccgcgctc gagacggcgt cgccgcaggt cttgtcgcgg gggaaggtcg cacggtcgac
     9001 gcaggcgacg cgcaggccca tgcgcgccag aaaggtgccc gcggcggcgc ccgccggtcc
     9061 ggcgccgacg atcaggacgt cgctgcggcc ggtgagcatg cgcgtgcaag gatagggatc
     9121 gcaggggtcg acgacaagcc ggtcaagcag gtttcgcgta gcatgcgacg cgtgtccgcc
     9181 cccctgcgcc gcgtgtggct cacgctgatc ggcctgcata tcctgctgct ggcctacgcg
     9241 ctgccgatcg gcgtgatcct cggcgactcg ccgctcggtg ggcccgacta tcagacgcac
     9301 tttcaacaca cgcacacgct gctgcaggtg caggccgagt tcgggcgggc gtgggcctac
     9361 gacccgaacc tgctcgccgg gcacccgacc gggctgatct tcgacgtcga caacaagctg
     9421 cacttcgggt ggacctcgat gctggtgcgc ctcggcgtgc cgctgccgac cgcgttcaat
     9481 ttgttcgcgt ggctggcctg cgcgctcgcg ccggtgtcgc tgtggctggc ggctcgcctg
     9541 ctgcggatgg aggtcggcgc gcgcgcgctg gccttcgggc tcggggtgct ggtgtggagc
     9601 ttcgacccga cggcccgctt ctgctgggcc ggcggcatga tctcgttcgc gttcgcggcg
     9661 catgtgtcga cggtcgtgat cgcggtgatg catcgcctgc tcgccgacgg cggccggcgc
     9721 ctcgcggtcg cgctgctggt gctgttgccg ctggtgttgc gcatgcacgt gtggagcttt
     9781 gcgatcctcg tggtgccgtt gacgggcatg tacctgcgcg cgtggcggca gatcccggcg
     9841 cgacggcacg ccgtcgtgtg gggcgctgcg ttgctcgggt tgctcgtcaa tctggactgg
     9901 ctgctgccgg cgctggcgca ccgtgacttg atcgtgccgt cggcgcggct cgggcaggcg
     9961 acgccgccgt atctgttatt cgattatctc gaattgctgg tcgacccgct gcgcaccggc
    10021 ttcgtcatgc agcgcacgct gctgcgcgcg ctggtggtga tcgcggcggt cgggacgttg
    10081 tggagctggc gcggggatgc caggcgcttc accggcgggc tgacgctcgc gtggctgctc
    10141 gggctgacct acgcgggcgc gctggtgccg ggggtgcagg cgaccgagcc gtatcgcttc
    10201 gcggtgccgc tggtgctgtg ggcgacggtg ctcgcgggca cgtggctgct gcgcgcggtc
    10261 gaggggctgc gcgggctgca gggcgcggcg cgcggggcgg tgctcgggct gctgatcctc
    10321 gtgctgccgc gcctgtatca ccagttgatg ccgtacctga cgatgttgag cccggggccg
    10381 gcgccgacgg ggcacgccgc gcgcagcctg ccgagcgcgc gcctcgaggg ggtgccggcg
    10441 gacttcgagg cggtggcgac ctggctcgaa ggacagccca ggggcgggcg cgtgctggtc
    10501 cagtatcacc cgctcgggga atatctgcgg tgggcgagcg actggccggt gctcggagga
    10561 ttttacgatc gcaggatgat cttccaggac gccaatttgt tctacttcgc ggcgggcgac
    10621 gaccgctatc gagatggttt ggcggcgtac ctcgagcgct acaatgtcag tcatctggtg
    10681 atgacatatc cgtatgtcgc ggaggtcgag cggcgcacgg acctgttcga gcccgcgggg
    10741 atccaggggg gcgtgcaccg ggtgtacacc gtgcgaaagc gcggtagtta ttttgtcgag
    10801 ggggtcggtg aggtgcaggc ggggctcaat cgcatcgcgg taagggcggc ccggccggcg
    10861 ccgggcagtc aggcgctgac gctgcgcttt cactacatgg acgagctgcg ctgcgcgccc
    10921 ggctgtcgcg tcgagcgggc cgaggtcgag ggcgacacgg cgggcttcgt gcgcgtggtc
    10981 ggtgagccgg tgctgccggc cgagttcgtg gtctcactgg agtactgagg gccgcttcgt
    11041 gttagcgtgc gcgccgatgg ctccggtccg ctatcggctg caccaggccg actgtctcgc
    11101 gtggatggac gcgcagccgg aatgctcggt gcacgcgatc gtcaccgacc cgccgtatgg
    11161 cctgcgcgag tacacggcgc cggagacggc caagctgcgc agcggcaagg ggggcgtgtg
    11221 gcggatcccg ccggcgctcg acggctgcaa gcgggccccg ctgccgcgct tcacggtgct
    11281 cagcgccgcc gaccgcgagg cgctgcaggc gttcttcgcc gcgttcgcgg cgcgggtgat
    11341 gcgggtgctg gtgccgggcg ggcacctgtt cctcgccgcg aacccgctgc tgtcgacgga
    11401 gatctacgtg ccattgatgg cggccgggct cgagaagcgc ggtgaggtga tccgtctggt
    11461 ccagaccctg cgcgggggcg atcgcccgaa gaacgcgcac gctgaatttt cagaggtatc
    11521 ggtgatgccg cgctcgggct gggagccgtg ggggctgttt cgccggccct gcgaggggcg
    11581 cgtgcaggac aacctgcggc ggtggaagac gggcgggctg cggaggatct ccgaggatct
    11641 gccatttagt gatgtcatcg cctcggcgcc gacgcagccg gccgagcgcg cgatctcccc
    11701 gcacccctcg ctcaagccgc aggcgttcat gcggcagatc gtccgtgccg cgctgccgct
    11761 cggcgagggc gtcgtgctcg acccgttcat gggcggcggc tcgacgatcg ccgcggcggt
    11821 cgcggtcggc tacgagagca tcggcgtcga gctcgacccg gccttcttcg cggtcgcgga
    11881 ggcggcgatc cctcggctcg cgcagctgcc ggtgaagctg cgcgggtgaa ccgcggagct
    11941 cactgctcgg cgtcgtcctc gatgcgctcg atggcccagg cgaggcggtc gcgcgcggcg
    12001 ctgtcggtct cggcctcgag cgcggtggtg agggccggga ggtcggcggg cttgccgagc
    12061 ttgccgaggc cctcggcggc gagcaggcgc acgcgtgggt cggggttggc gagggtctcg
    12121 cggaacaggt cgtgcagggc cgggctggcg gtccacgccc gcagcgatcg ccgggcgatc
    12181 gcccgcacct ggtcgtcggc gtcgcgcagc gcgaggccga tgagaggcgc gtgcgcgggg
    12241 tcgtgggcgg tcagcgcgtg cgcggcgacg cggcgtgact cgaggtcgcc gccgagccag
    12301 cgctgtagta gctccgcgct ggcgagggtg cgcacggcct ggcgatagcg gacgacgtcc
    12361 tgaccctcca tcgtgtcggt catcgcggcg accagcgcag gcagggcccg ctcgtcgccc
    12421 gtggcggcga ggtgctgcgc ctcggcgggg gtcggtcgtt gggagaaggc ggcgatgcgg
    12481 gcgaggaggg tggcc
//
LOCUS       DASWNJ010001754         1933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_490654, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001754 DASWNJ010000000
VERSION     DASWNJ010001754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490654"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="VGB85_29355"
     CDS             complement(<1..1145)
                     /locus_tag="VGB85_29355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138241.1"
                     /translation="MSHVPGSPVPGDTSMTRLDRVRRLVRAVQRRERLAEATRLLTDI
                     ALPAGLLLAAVGLAVMHRWGTQQAPLTWLPWLGLVPVPLALLWAAFRPRPARNAARRL
                     DYHYGLHDLLGGALELAAQPPADDPRSADMVALLTADAEAAAANIDPRPVVPLPRPGA
                     RALLLGWAGALAVLAAMFVPPAPTPEVEQDGTSTIPTDATAVAARPSPDRALAEPLRE
                     DLRALKGGQDTAAKIAEAMLEVLDAFGRGEIDRAAAFAKLEQLEQQLAEAEAELEATQ
                     EADPAILAEGMRELAEALEQEEITKPAGEALARGESEQAEQALAEAEDAAAASEANMQ
                     SLERAMAAAEKALGKAAGENTDTAAELAEAERRLKRQQKQPAADPEE"
     gene            complement(1165..>1933)
                     /locus_tag="VGB85_29360"
     CDS             complement(1165..>1933)
                     /locus_tag="VGB85_29360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138242.1"
                     /translation="ARTYLVEGDAQAAYLAWVSGSGGLTSISDEVFLATGNQALDLAD
                     MVDYPVLARSLQLPYADGAATIAALVRERGWAGVDALYKQLPTTTEQMLHIDKLLARE
                     APIAVVADPAALATKIPGHKVVWQDNVGEASLLAMLADVEPAPIARAAAAGWGGDRYL
                     VLEREPAGVAPIVLGLIAWDSEADAAEFTETFQLYLEKTMPGAFVLERRGAQVLYASQ
                     VRDAATRAALHTHAWTAFTVAGKPAGAGKTAVKARAA"
BASE COUNT          212 a          661 c          790 g          270 t
ORIGIN      
        1 tgctcctcgg ggtcggcggc gggctgcttc tgctggcgct tgagccggcg ctcggcctcg
       61 gcgagctcgg cggcggtgtc ggtgttctcg ccggcggcct tgccgagcgc cttctcggcg
      121 gcggccatcg ctcgctcgag agattgcatg ttcgcctcgc tcgcggccgc ggcgtcctcg
      181 gcctcggcga gcgcctgctc ggcctgctcg gactcgccgc gcgcgagcgc ctcgccggcg
      241 ggtttggtaa tttcctcctg ctcgagcgcc tcggcgagct cgcgcatgcc ctcggcgagg
      301 atcgccgggt cggcctcctg ggtcgcctcg agctcggcct cggcctcggc gagctgctgc
      361 tcgagctgct cgagcttggc gaaggcggcg gcgcgatcga tctcgccgcg accgaatgca
      421 tcgagcacct cgagcatcgc ctcggcgatc ttggcggcgg tgtcctggcc gcccttgagc
      481 gcgcgcaggt cctcgcgcag cggctcggcg agggcgcggt cggggctcgg gcgcgcggcg
      541 acggcggtgg cgtcggtcgg gatggtcgag gtgccgtcct gctcgacctc gggcgtcggc
      601 gcgggcggga cgaacatcgc cgcgagcacc gcgagcgcgc cggcccagcc gagcagcagg
      661 gcgcgcgcgc cggggcgcgg cagcgggacg accgggcgcg ggtcgatgtt cgcggcggcc
      721 gcctcggcgt cggcggtcag cagcgcgacc atgtcggcgc tgcgcgggtc atcggccggg
      781 ggctgggccg cgagctcgag ggcgccgccg agcaggtcgt gcaggccgta gtgatagtcg
      841 aggcggcgcg cggcgttgcg ggcgggtcgc gggcgaaacg cggcccacag cagcgcgagc
      901 ggcacgggca ccaggccgag ccatgggagc caggtgaggg gcgcctgctg cgtgccccag
      961 cgatgcatca ccgcgagccc gaccgcggcg agcagcaggc cggccggcag cgcgatgtcc
     1021 gtcagcagcc tggtcgcctc ggcgagccgc tcgcgccgct gcacggcgcg aaccaggcgt
     1081 cgcacgcggt cgagccgcgt catcgacgtg tctccgggga caggtgaacc tggcacatgg
     1141 gacatgttcg catgatcagg tgagtcaggc ggccctggcc ttcacggcgg ttttgccggc
     1201 gcccgcgggc ttgccggcga cggtgaaggc ggtccaggcg tgggtgtgca gcgcggcgcg
     1261 ggtggccgcg tcgcggacct gcgaggcgta gagcacctgc gcgccgcggc gctcgagcac
     1321 gaaggcgccg ggcatcgtct tctcgaggta gagctggaag gtctcggtga actcggcggc
     1381 gtcggcctcg ctgtcccagg cgatcaggcc gagcacgatc ggggcgacgc cggcgggctc
     1441 gcgctcgagc acgaggtagc ggtcgccgcc ccagcccgcc gcggcggcgc gggcgatcgg
     1501 ggcgggctcg acgtcggcga gcatcgcgag caggctggcc tcgccgacgt tgtcctgcca
     1561 cacgaccttg tgcccgggga tcttcgtggc gagcgcggcc gggtcggcga cgacggcgat
     1621 cggggcctcg cgggcgagca gcttgtcgat gtgcagcatc tgctcggtgg tcgtcggcag
     1681 ttgcttgtag agcgcgtcga cgccggccca gccgcgctcg cgcacgaggg cggcgatcgt
     1741 cgcggcgcca tcggcgtagg gcagctgcag cgagcgtgcg agcacggggt agtcgaccat
     1801 gtcggcgagg tcgagggcct gattgccggt ggcgaggaag acctcgtcgc tgatgctggt
     1861 gaggccgccg gagccgctga cccacgcgag gtacgcggcc tgggcgtcgc cctcgaccag
     1921 gtaggttcgc gcc
//
LOCUS       DASWNJ010001755         2495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_525098, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001755 DASWNJ010000000
VERSION     DASWNJ010001755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525098"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..908
                     /gene="yihA"
                     /locus_tag="VGB85_29365"
     CDS             213..908
                     /gene="yihA"
                     /locus_tag="VGB85_29365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEY0138243.1"
                     /translation="MTTAQIKSSPAGWRLVAASFEKSAPKLRDAPEGDLPEIAVTGRS
                     NVGKSSLLNAFAHQKGLARVSRTPGRTQLLNFFKMQLRHDRHGDFEFRWADLPGYGYS
                     KTPQSVRDGFGPMIEGYLQGRTILRGLVLLIDCRRDVSDHDHALLDFMNSRSLPVLVC
                     ATKADKLSKHERGVVSNGIAKSLGIKPRQVLVTSADTGLGLGDEGKEGGLARELAELL
                     KQTVGAWPRPDAP"
     gene            1023..1826
                     /locus_tag="VGB85_29370"
     CDS             1023..1826
                     /locus_tag="VGB85_29370"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEY0138244.1"
                     /translation="MSAAQFEARVTANTRLGGGYFTLELEADAPTPVAGARPGQFVML
                     RGAWGRELLNPRAFSVLHAVGPRLAILAKPYGLGTRRMQTLRPGDPMTVTGPLGVGFP
                     DPSADVRDLLVAGGVGLPPLHLQARVAAAAGLAGQQELYYGGRDRGDLVMLDDFAAWN
                     VEAHLATEDGSLGVRGRVTDALRPRLARARDTGERVRILGCGPTPMLAAVRHLGLEHG
                     VETLLCLEENMACGFGVCLGCAVPVHGVKPYRYCCTDGPVFDAREVRWP"
     gene            1855..>2495
                     /locus_tag="VGB85_29375"
     CDS             1855..>2495
                     /locus_tag="VGB85_29375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase catalytic subunit"
                     /protein_id="HEY0138245.1"
                     /translation="MSGARPTAIAPADVELACEFGPLRLQSPLLAASGCFAYGQQFAA
                     FTDLRALGGLSTKGISPLPRHGNPLPRICETAAGMLNSIGLENVGAEAFLAEKLPFLR
                     DLGISVWVNFFGTDFPTYIECARTLGNAPGIDALELNISCPNIKHGGIEFGTVPEVAH
                     RLTRACVEVSAAPIVVKLTPNAGDIVAMADAVRQAGAMGVSLINTITGMAIDVR"
BASE COUNT          342 a          990 c          798 g          365 t
ORIGIN      
        1 ctcgctgccg ggtgtcacga ccacgcgcga cagctcgtcg gccgacacca ccgggatccc
       61 gcagtcctcg aagtacgccg cggccgcgga cttgccgctg ccgatcccgc cggtcagtcc
      121 gtagatgtcc atgccgcacc agccttacaa aagccccgcg tcctgtacag ggccgggcca
      181 gcgcgcctgc catggtctat gctcccggcc ccgtgacgac cgcccagatc aagtcctctc
      241 ccgccggctg gcgtctggtc gccgcgagct tcgagaagag cgcgccgaag ctgcgcgacg
      301 cccccgaggg tgacctcccc gagatcgccg tcaccggccg gtccaacgtc ggcaagtcga
      361 gcctgctcaa cgccttcgcc caccaaaagg gcctcgcccg cgtcagtcgc acccccggcc
      421 gcacgcagct gctcaacttc ttcaagatgc agctgcgcca cgacaggcac ggcgacttcg
      481 agtttcgctg ggccgacctg cccggctacg gctactccaa gaccccgcag tcggtgcgtg
      541 acggcttcgg gccgatgatc gagggctatc tgcaggggcg cacgatcctg cgcggcctcg
      601 tcctgctgat cgactgccgc cgcgacgtca gcgaccacga ccacgcgcta ctcgacttca
      661 tgaactcgcg cagcctgccc gtgctggtgt gcgcgaccaa ggccgacaag ctcagcaagc
      721 acgagcgcgg cgtcgtcagc aacgggatcg ccaagagcct cggcatcaag ccgcgccagg
      781 tcctcgtcac cagcgccgac accggcctcg gcctcggcga cgagggtaaa gagggcggcc
      841 tcgcccgcga gctcgccgag ctgctcaagc agaccgtcgg cgcctggccg cggcccgacg
      901 cgccctgagc ctcgcggcga tctcccgtcg gtttagcacc gcgcaaggtc acgtcggttt
      961 gcgcgccggt cgcgggcgtt tgtgctttac gcgccggccc gcgctggcct atgcccccgt
     1021 gcatgtccgc ggctcagttt gaagcgcgcg tcacggccaa cacccggctc ggcggcggct
     1081 acttcaccct cgagctcgag gccgacgcgc cgacccccgt cgccggcgcg cgcccggggc
     1141 agttcgtgat gctgcgcggc gcctggggcc gcgagctgct caatccccgc gccttttcgg
     1201 tgctgcacgc cgtgggcccg cgcctggcga tcctcgccaa gccctacggc ctcggcaccc
     1261 ggcgcatgca gaccctgcgg cctggtgatc cgatgaccgt caccggcccg ctcggcgtcg
     1321 gcttccccga tccctccgcc gacgtccgcg acctgttggt cgccggcggc gtcggcctgc
     1381 cgcccttgca tctacaagcg cgcgtcgccg cggccgccgg cctcgctggc cagcaagagc
     1441 tctactacgg cggccgcgac cgcggcgacc tcgtgatgct cgacgacttc gccgcctgga
     1501 acgtcgaggc ccacctcgcc accgaggacg gcagcctcgg cgtgcgcggc cgcgtcaccg
     1561 acgccctgcg cccgcgcctc gcccgcgccc gcgacaccgg cgagcgggtc cggatcctcg
     1621 gctgcggccc gacgccgatg ctcgccgcgg tgcgccacct cggcctcgag cacggcgtcg
     1681 agaccctcct gtgcctcgag gagaacatgg cctgcggctt cggcgtgtgc ctcggctgcg
     1741 ccgtgcccgt gcacggcgtc aagccatacc gctactgctg caccgacggc ccggtgttcg
     1801 acgcccgcga ggtccgctgg ccatgagacc atgtccgcaa agccaggtgc tgtcatgagc
     1861 ggcgcgcgcc cgaccgcgat cgcccctgca gacgtcgagc tcgcctgcga gttcggcccg
     1921 ctgcgcctcc agagcccctt gctggccgcc tcgggctgct tcgcctacgg ccagcagttc
     1981 gccgccttca ctgacctgcg ggcgctcggc ggcctgtcga ccaaggggat ctcgccgctg
     2041 ccgcgccacg gcaacccgct gccgcggatc tgcgagaccg ccgcgggcat gctcaactcg
     2101 atcggcctcg agaacgtcgg cgccgaggcg ttcctcgccg agaagctgcc gtttttgcga
     2161 gatctcggga tctcggtgtg ggtcaacttc ttcggcacgg acttccccac ctacatcgag
     2221 tgcgcccgca ccctcggcaa cgcccccggg atcgacgcgc tcgagctcaa catcagctgc
     2281 ccgaacatca agcacggcgg gatcgagttc ggcaccgtgc cggaggtcgc ccaccgcctg
     2341 acccgcgcct gcgtcgaggt cagcgccgcc ccgatcgtcg tcaagctgac ccccaacgcc
     2401 ggtgacatcg tcgccatggc cgacgccgtg cggcaggccg gcgcgatggg cgtatcgctg
     2461 atcaacacga tcaccggcat ggcgatcgac gtgcg
//
LOCUS       DASWNJ010001756         3060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_30985, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001756 DASWNJ010000000
VERSION     DASWNJ010001756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30985"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            394..825
                     /gene="tssE"
                     /locus_tag="VGB85_29380"
     CDS             394..825
                     /gene="tssE"
                     /locus_tag="VGB85_29380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HEY0138246.1"
                     /translation="MAGRSDGEESGQSSPTMRRGLLARLSSGGQRVSEVESIIAHVYE
                     LLNTRVGESLAAPDLGIIDFADLVHNFPEATQVLQQSIRATIMKYEPRLRSVSVTPTP
                     SNDPLSLAFEISGRLASGSQRGPFRLRTELTSTGKMQISGR"
     gene            839..2572
                     /gene="tssF"
                     /locus_tag="VGB85_29385"
     CDS             839..2572
                     /gene="tssF"
                     /locus_tag="VGB85_29385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HEY0138247.1"
                     /translation="MSFSHYYKSELALIHDLARNFAEANPATAGLLKERSSDPDVERL
                     IEAFAFQAAGIRARMDSVMPDIVQGLAELLLPHYLRPLPAATIVEFAPNLRALRSRHR
                     LPAGRNLTTKPVEGTACRFRTCFDVDLLPLELVDAVLDDGVAAKPVLRMTLRLSEAGK
                     AALPDCNRLRIFLHHGEPTLPATLMLWFARHLTGVSVQGAPGEPPTQLGRQAVELVGL
                     SRNLQVLPWPDTAPSGFRLLFEYFTLPSRFHFLDLVGLDRARKAGPEIVVNFEFARPP
                     ALPAAVKRENLRLYCTPALNLFEASAEPLAYSPLEREHLLRADGVDPRHMEVCEVRSV
                     VGVGRGANMRRTFLPFYRFAHGGQSTRGAPFYSLRRTPAIDGGVDTYINLDEPRGAPP
                     LAHEEAVSVELLCTNRHLPRGLQPGAVTESAPGSMFRSYENITPVTAPVRVPLGTDAL
                     WRLLSHLALGQRGLADVESLRSMLALYNVQGLNQMEDGRINERQIEAIRKVTREMVTR
                     LVHGVPIRAVRTRVELDEAGFPSIGNAFIFSALLSELSGSLVELNAASEVVATLSASK
                     MEFPWPVQIGS"
     gene            2548..>3060
                     /locus_tag="VGB85_29390"
     CDS             2548..>3060
                     /locus_tag="VGB85_29390"
                     /inference="COORDINATES: protein motif:HMM:NF018675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HEY0138248.1"
                     /translation="MAGPDRELDAVWKVWRERLLAAPARHEFVDVVDLLARMTDKVTL
                     RGSTSLGYSSGELTGVELTEGPGKPAATVTTAFFGLAGETTPLPLGYAEEADRDDEHG
                     NIVRGLLDLFNHRLLGLLHAAITGLDYPHSFREDGKDPWTQAILALLGVSQERTALPP
                     ALLLRLAPIFA"
BASE COUNT          432 a         1153 c         1039 g          436 t
ORIGIN      
        1 agccgacgcc gcccaagccc gccgacaccg gcggcgtctg atccggggcg accgtgtcac
       61 ccgccgcgcg tatcaccgac atgcacacgt gcccgctgcc gaccgacgtc gtcgggcgcg
      121 tcgctctcga actcgagctt cgccgaggac tcttccgagg ccgacgccga ggcgctcgtc
      181 gagcgcctga tggggggtcg caagtgagcg tccgccagga tcctcgtggc gctgacgagc
      241 ttgcggcggc gcgcgaacat gggcgcgatg cggacggcgc cgaggcactg gcagtttcgc
      301 tccgcggccg cgcgttcgag gcgcgccatc ggccgcggac cgctggtata acggagcggc
      361 tggcgggccg caggccggcg aaggggcgtg gtgatggcag ggaggagcga cggtgaagaa
      421 tccggccagt cgagcccgac gatgcggcgt ggtctgctcg cgcggctctc gagcggtggc
      481 cagcgcgtca gcgaggtcga gtcgatcatc gcccacgtgt acgagctgct caacacgcgg
      541 gtcggcgagt cgctcgcggc gcccgatctc gggatcatcg acttcgcgga cctcgtgcac
      601 aacttccccg aggccacgca ggtgctgcaa cagagcatcc gcgccacgat catgaagtac
      661 gagccgcgcc tgcgcagcgt gtcggtgacg ccgaccccga gcaacgaccc gctgagcctc
      721 gccttcgaga tctccgggcg gctggccagc ggctcgcagc gcgggccatt tcgcctgcgc
      781 accgagctga cctccaccgg taagatgcag atctccggac gttgagccga gaaccacgat
      841 gagcttctct cactattaca agagcgagct ggcgctcatc cacgacctcg cgcgcaactt
      901 cgccgaggcc aaccccgcga ccgccggcct gctcaaggag cgcagcagcg accccgacgt
      961 cgagcgcctg atcgaggcgt tcgcgttcca ggccgcgggc atccgggcgc gcatggactc
     1021 ggtgatgccg gacatcgtcc agggcctcgc cgagctgctg ctgccgcact acctgcgacc
     1081 gctgccggcc gcgacgatcg tcgagttcgc cccgaacctg cgggcgctcc gcagccgcca
     1141 ccgcctgccg gccgggcgca acctgacgac caagccggtc gagggcaccg cgtgccggtt
     1201 ccggacctgc ttcgacgtcg acctgctgcc gctcgagctc gtcgacgcgg tgctcgacga
     1261 cggcgtcgcc gccaagccgg tgctgcgcat gacgctgcgc ctcagcgagg ccggcaaggc
     1321 cgcgctgccg gactgcaacc gcctgcgcat cttcctgcac cacggcgagc cgacgctgcc
     1381 ggcgaccctg atgctgtggt tcgcgcgcca cctgaccggc gtatcggtgc aaggcgcccc
     1441 cggtgagccg ccgacccagc tcgggcgcca ggcggtcgag ctcgtgggtc tgtcgcgcaa
     1501 cctgcaagtg ctgccgtggc cggacaccgc gccctcgggc tttcgcctgc tgttcgagta
     1561 cttcacgctg ccgtcgcgct tccacttcct cgacctcgtc ggcctcgacc gcgcgcgcaa
     1621 ggccggcccg gagatcgtcg tcaacttcga gttcgcgcgc ccgcccgcgc tgccggccgc
     1681 ggtcaagcgc gagaacctgc gcctgtactg cacgcccgcg ctcaacctgt tcgaggcgag
     1741 cgccgagccg ctggcctaca gcccgctcga gcgcgagcat ctgctccgcg ccgacggcgt
     1801 cgacccgcgg cacatggagg tctgcgaggt gcgatcggtc gtcggggtcg gccgcggcgc
     1861 caacatgcgg cgcacgttcc ttccgttcta tcgcttcgcc cacggcggcc agtcgacccg
     1921 cggcgccccg ttctactcgc tgcgtcgcac ccccgcgatc gacggcggtg tcgacacgta
     1981 catcaacctc gacgagccgc gcggcgcccc gccgctggcc cacgaggagg cggtgtccgt
     2041 cgagctgctg tgcaccaacc gccacctgcc gcgcgggctc cagcccggcg cggtcaccga
     2101 gtcggcgccg ggctcgatgt tccgcagcta cgagaacatc acgccggtca ccgcgcccgt
     2161 gcgcgtgccg ctcggcaccg acgcgctgtg gcgcctgctc tcgcacctgg cgctcggtca
     2221 acgcggtctg gcggacgtcg agtcgctgcg ctcgatgctc gcgctctaca acgtgcaggg
     2281 cctcaaccag atggaggacg gccgcatcaa cgagcgccag atcgaggcga tccgcaaggt
     2341 gacgcgcgag atggtgaccc gcctcgtcca cggcgtgccg atccgggcgg tgcgcacccg
     2401 cgtcgagctc gacgaggccg gcttcccgag catcggcaac gccttcatct tcagcgcgct
     2461 gctcagcgag ctgagcggct cgctcgtcga gctcaacgcc gccagcgagg tcgtcgccac
     2521 cctctcggca tcaaagatgg agttcccgtg gccggtccag atcgggagct agacgccgtc
     2581 tggaaggtct ggcgcgagcg gctgctcgcc gcaccggcgc gccacgagtt cgtcgacgtc
     2641 gtcgacctgc tcgcgcgcat gaccgacaag gtgacgctgc gcggctcgac ctcgctcggc
     2701 tactcgagcg gcgagctcac cggggtcgag ctgaccgagg gccccggtaa accggccgcg
     2761 acggtgacga ccgcgttctt cgggctcgcc ggcgagacca cgccgctgcc gctgggctac
     2821 gccgaggagg ccgaccgcga cgacgagcac ggcaacatcg tgcgcggcct gctcgacctg
     2881 ttcaaccacc gcctgctcgg cctgctgcac gccgcgatca ccggcctcga ctatccgcac
     2941 agcttccgcg aggacggcaa ggacccgtgg acgcaggcga tcctggcgct gctcggcgtg
     3001 tcgcaggagc gcaccgcctt gccgccggcg ctgctgctgc ggctcgcgcc gatcttcgcc
//
LOCUS       DASWNJ010001757         3468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_414179, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001757 DASWNJ010000000
VERSION     DASWNJ010001757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414179"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2871
                     /locus_tag="VGB85_29395"
     CDS             <1..2871
                     /locus_tag="VGB85_29395"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY0138249.1"
                     /translation="IPGQAMYYATAYQVGSTVLGLLVESQDGRPTKIEGNPRHSGSNG
                     ATDMWAQASVLSLYDPDRCRVPHRLGADGKHVPSDWDTVTEELGATLKELRAAQGAGV
                     ALVVPNVLSPTYRDTLRGFQEKWPRARIFIDDPAAANNAVAGAELLAGPGARPTYHLA
                     GVKLIASFDSDFIATEFDHVRLQREFAEGRAVTSPADAPEMSRLYVLEPHLTVTGAQA
                     DNRLRVRGGEVGGLLAALADRLLAEKDVRLPPGGDQLRGALPKATLTPEQDKFITALA
                     ADITASRTARRPSVIVVGERQPPWVHALGFLLNYALGNGANSLRLRLDDLAVATEPMA
                     ALAAGLRDGSITQVITLETNPAYDGSGELALAEALKGKVLLHLGQHIDETGQLATWCL
                     PAAHYLESWGDLEAADSTISIVQPLIAPLHGGRSAIEMLAWIANDAQIDGYSLVQGYW
                     RTVLGPMYSDRVWRRWLHDGVAQGIPRAGTTPSPRDLGGLTTALTAAGSAEAAGAGYE
                     LNFHLDPKIADGRHANNGWLQEAPHPITKLTWDNAAYISIETARKLEVANCDNLRIAV
                     GGREVIIPVWIAPGQADDTISLPLGYGRNTIGAVAKDAGVNVYGLQGGLNPWIVSGAD
                     VTRQGTRRMVYSTQNHGSLDPGVDPASTNDDSQGLNPGRGYPERPIVRQTTVDGFKKD
                     PDFSQKGDLIAKENLRSLWDHNTHPDLGPPAYIGAQQWGMAIDLNKCTGCTACTVACQ
                     AENNIPVVGRSQVANGREMHWIRIDRYYTGPKEAPEAVVQPMLCQHCETAPCENVCPV
                     NATAHSPEGLNDMAYNRCIGTRYCANNCPYKVRRFNFFNYNKDPVNDNEMVRMQKNPD
                     VTVRFRGVIEKCSYCVQRIQEAKIQAHTAGEDTVKDGVIVTACAQVCPSAAITFGDVA
                     DPNSKVSQVKKNGRNYGVLTDLNTRPRTTYLARVRNPNPELV"
     gene            2878..>3468
                     /gene="nrfD"
                     /locus_tag="VGB85_29400"
     CDS             2878..>3468
                     /gene="nrfD"
                     /locus_tag="VGB85_29400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEY0138250.1"
                     /translation="MATAGHTHSSPPGTPGLEPHGSPALQAIDRDRLDPLTGRKALVE
                     EARTFHDVTEAVANPIEAPNSRWWIAFLMSLSMLGVLGIALGYLFWMGTGIWGLNNPV
                     AWGWAIVNFVFWVGIGHAGTLISAVLFLFRQKWRTSINRASEAMTIFAVICALLFPTV
                     HTGRPWFIYWPLPLPNQMDMWPNFRSPLLWDVFAVST"
BASE COUNT          567 a         1376 c         1063 g          462 t
ORIGIN      
        1 atccccggcc aggcgatgta ctacgcgacc gcctaccagg tcggcagcac cgtgctcggc
       61 ctgctcgtcg agtcgcagga cggccgcccg accaagatcg aaggcaaccc gcggcacagc
      121 ggctccaacg gcgcgacgga catgtgggcc caggcctccg tgctgtcgct ctacgacccg
      181 gaccgctgcc gcgtcccgca tcgcctcggc gcggacggca agcacgtccc gagcgactgg
      241 gacaccgtca ccgaggagct cggcgcgacc ctcaaggagc tgcgcgcggc ccagggcgcc
      301 ggcgtggcgc tcgtcgtccc caacgtgctc tcgccgacct accgcgacac cctgcgcggc
      361 ttccaggaga agtggcccag ggcgcgcatc ttcatcgacg accccgccgc ggccaacaac
      421 gccgtcgccg gcgccgagct gctcgcgggc cccggcgccc gtccgaccta ccacctcgcc
      481 ggcgtcaagc tgatcgcctc gttcgactcg gacttcatcg ccaccgagtt cgaccacgtt
      541 cgcctgcagc gcgagttcgc cgagggccgc gcggtcacca gccccgccga cgcccccgag
      601 atgagccggc tctacgtgct cgagccgcac ctgaccgtca ccggcgccca ggccgacaac
      661 cgcctgcgcg tgcgcggcgg cgaggtcggt ggcctgctcg ccgcgctcgc cgatcgcctg
      721 ctcgccgaga aggacgtccg cctgcccccc ggcggcgacc agctgcgcgg cgccctaccc
      781 aaagcgaccc tgacgcccga gcaggacaag ttcatcaccg cgctcgcggc cgacatcacc
      841 gcgtcccgca ccgcccgccg cccgagcgtg atcgtcgtcg gcgagcgcca gccgccgtgg
      901 gtccacgcgc tcggcttcct cctcaactac gccctcggca acggcgccaa ctccctccgc
      961 ctgcggctcg acgacctcgc ggtcgcgacc gagccgatgg cggcgctcgc cgccggcctg
     1021 cgcgacggct cgatcaccca ggtgatcacg ctcgagacca acccggcgta cgacggctcc
     1081 ggcgagctcg ccctcgccga ggcgctcaag ggcaaggtgc tgctgcacct cggccagcac
     1141 atcgacgaga ccggccagct cgccacctgg tgcctgccgg ccgcgcacta cctcgagagc
     1201 tggggcgacc tcgaggccgc cgactcgacg atctcgatcg tccagccgct gatcgccccg
     1261 ctgcacggcg gacgcagcgc gatcgagatg ctcgcgtgga tcgccaacga cgcccagatc
     1321 gacggctaca gcctggtcca gggctactgg cgcaccgtgc tcggcccgat gtactcggac
     1381 cgcgtgtggc gtcgctggct gcacgacggc gtcgcccagg gcatcccgcg cgccggcacc
     1441 acgccgtccc cgcgtgacct cggcggcctg accaccgcgc tcactgctgc gggttcggcc
     1501 gaggcggccg gcgccggcta cgagctcaac ttccacctcg acccgaagat tgccgacggt
     1561 cgccacgcca acaacggctg gctgcaggag gccccgcacc cgatcaccaa gctgacctgg
     1621 gacaacgcgg cctacatcag catcgagacc gcccgcaagc tcgaggtcgc caactgcgac
     1681 aacctgcgga tcgccgtcgg cggccgcgag gtgatcatcc ccgtgtggat cgcgcccggc
     1741 caggccgacg acaccatctc gctgccgctc ggctacggcc gcaacacgat cggcgccgtc
     1801 gccaaggacg ccggcgtcaa cgtctacggc ctgcaaggcg gcctcaatcc ctggatcgtc
     1861 agcggcgccg acgtgacccg ccagggcacg cgccggatgg tctactcgac gcagaaccac
     1921 ggctcgctcg acccgggcgt cgacccggcc tcgaccaacg acgacagcca gggcctcaac
     1981 cccggccgcg gctaccccga gcgcccgatc gtccgccaga ccaccgtcga cggtttcaag
     2041 aaggatccgg acttctccca gaagggcgac ctgatcgcca aggagaacct gcgctcgctg
     2101 tgggaccaca acacccaccc cgacctcggg cccccggcct acatcggcgc ccagcagtgg
     2161 ggcatggcga tcgacctcaa caagtgcacc ggctgtaccg cgtgcacggt cgcctgccag
     2221 gcggagaaca acatccccgt cgtcggccgc tcgcaggtcg ccaacggccg cgagatgcac
     2281 tggatccgca tcgaccgcta ctacaccggc ccgaaggagg cccccgaggc ggtcgtccag
     2341 ccgatgctgt gccagcactg cgagacggcc ccctgcgaga acgtgtgccc ggtcaacgcg
     2401 accgcccaca gccccgaggg cctcaacgac atggcctaca accgctgcat cggcacgcgg
     2461 tactgcgcca acaactgccc gtacaaggtc cggcggttca acttcttcaa ctacaacaag
     2521 gaccccgtca acgacaacga gatggtccgg atgcagaaga acccggacgt caccgtccgc
     2581 ttccgcggcg tgatcgagaa gtgctcctac tgcgtccagc gcatccagga ggccaagatc
     2641 caggcccaca cggccggcga ggacaccgtc aaggacggcg tcatcgtcac cgcctgcgcc
     2701 caggtgtgcc cgagcgctgc gattaccttc ggcgatgtcg ccgacccgaa cagcaaggtg
     2761 tcgcaggtca agaagaacgg ccgcaactac ggcgtgctga ccgacctcaa cacccgcccg
     2821 cgcacgactt acctcgcgcg ggtccgcaac cccaatccgg agctcgtgta aaccgccatg
     2881 gcaaccgctg gtcacactca ctccagccct ccgggcaccc cgggcctgga gccccacggc
     2941 tctccggctc tgcaagcgat cgaccgcgac cggctcgacc cgctcaccgg ccgcaaggcg
     3001 ctcgtcgagg aggcgcgcac cttccacgac gtcaccgagg ccgtcgcgaa cccgatcgag
     3061 gcgcccaaca gccgctggtg gatcgcgttc ctgatgtcgc tgtcgatgct cggcgtcctc
     3121 ggcatcgcgc tcggctacct gttctggatg ggtaccggca tctggggcct caacaacccg
     3181 gtcgcgtggg gttgggcgat cgtcaacttc gtgttctggg tcggcatcgg ccacgccgga
     3241 accctgatct ccgcggttct gttcctgttc cgccagaagt ggcgcacctc gatcaaccgc
     3301 gcctcggagg cgatgacgat cttcgccgtc atctgcgccc tcctgttccc gacggtccac
     3361 accggccgcc cctggttcat ctactggccg ctcccgctgc cgaaccagat ggacatgtgg
     3421 cccaacttcc gcagcccgct gctgtgggac gtgttcgccg tctcgacg
//
LOCUS       DASWNJ010001758         3309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_30991, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001758 DASWNJ010000000
VERSION     DASWNJ010001758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30991"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VGB85_29405"
     CDS             complement(<1..475)
                     /locus_tag="VGB85_29405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138251.1"
                     /translation="MHRLNLDRGWVVLALAAACGDSGRAESDSNPSPTGTSVTSISGT
                     GSSGETTTDLPTTGPGTTSGLPTTGTGAGAGEGEPCTASDGCTDGLVCAGGTCVPGEG
                     ECTSDDECTGDTYCCLAGCLPAGETGGVCIPFGTGPKGEVNDECVGDVSIGLFEPS"
     gene            complement(590..1843)
                     /locus_tag="VGB85_29410"
     CDS             complement(590..1843)
                     /locus_tag="VGB85_29410"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0138252.1"
                     /translation="MFASSPADPVPDEDDGARMIGRFKVLGLVGRGGFGEVYAAHDPE
                     LDRRVALKVVPPTEDRAAVARLHREAMTMARLSHPNVARVFDVGDVDGHLFIAMEFIR
                     GDDLRTWLSGQPRDWRAVREVFLAAGQGLAAAHREGIVHRDFTPEIVIIGEDGRARVI
                     DFGLAAPTISDLLPGHTAEDDETSDASRSDPGLGGTPAYMAPELHLGRPADARSDQYS
                     FCVALFEALHGRRPLLGRTWQTLSPQVLRGGLESVAASRKFPPHLRRTVLRGLSAAPE
                     RRFADMDALLAELMRDPLRQSQRRYLFAGAGLTALAGAVALWLSPPDAEPSPAPPSQL
                     RAPADTCARDPARCAARVDALQGQLQLARDLRDEGRGAQALQLLTALADTARTTLGAG
                     HPLSAEAERERAALAAALARSTAKP"
     gene            2044..3252
                     /locus_tag="VGB85_29415"
     CDS             2044..3252
                     /locus_tag="VGB85_29415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0138253.1"
                     /translation="MRTPDTGGKLLLLSSLYLAQGLPDGFFRQALPVLLRFEGVSLEG
                     IGLASLLMLPWALKFLWAPLVDRHGWSRFGRRRSWIVPLQALAVGLLLLLSEAEPQRG
                     LAPLLAGVLVINLLAATQDIATDGLAVTILRPEERGLGNGVQVAAYRLGMILSGGVLI
                     IFLEEIGWARTFYVMAGISALALVPLLMTREPPSPRPERAGLRVLADFVRQPGMRAWL
                     WTIAAFKLGEAFGVAMLRPLLVDRGVSLTTLGWITGTAGFIAGLAGALIGGAMVGVYG
                     RHRMLLIFGVVQAVAVASYVALAIGPLDNLLVYLLCTLEHLSSGMATAALFTMMMDAA
                     RPQAAGTDYTAQASVVVLSTGLGSAVSGFSAQALGYAGHFMLAATLCLLALIPVVLHG
                     RRVRAELSRG"
BASE COUNT          404 a         1143 c         1282 g          480 t
ORIGIN      
        1 ccgagggctc gaacaggccg atcgagacgt cgccgacgca ctcgtcgttg acttcgccct
       61 tgggcccggt gccgaagggg atgcacacgc ccccggtctc acccgcaggc aagcagcccg
      121 cgaggcagca gtaggtgtcg ccggtgcact cgtcgtcgct ggtacactcg ccctcgcccg
      181 gcacgcaggt gccaccggca cacacgaggc cgtcggtgca gccgtcgctg gcggtgcacg
      241 gctcgccctc accggcgccg gcgccggtgc cggtcgtcgg cagcccggag gtcgtgcccg
      301 gcccggtggt cggtagatcc gtcgtggtct cgccgctgct gccggtgccg gagatcgagg
      361 tgacgctcgt gccagtcggg gaggggttgc tgtccgactc ggcgcggccg ctgtcaccac
      421 acgcggcggc cagcgcgagc acgacccacc cgcgatccaa attcaagcga tgcatgcgtt
      481 cacgctacgc tcgcgtccgc gggagcgtca agccgccggg ccccacgcgc ggccctgcgc
      541 aggcgcgtgc tgcacatggc ctccgcgaca tgtccgatcg gacatgtgat cagggcttgg
      601 cggtcgagcg ggcgagcgcg gccgccagcg cggcgcgctc gcgctcggcc tcggcgctga
      661 gcgggtggcc ggcgccgagg gtcgtgcggg cagtgtcggc gagcgccgtc aacaactgca
      721 gggcctgggc cccgcggccc tcgtcgcgca ggtcgcgggc gagctgcagc tggccctgca
      781 aggcgtcgac gcgggcggcg cagcgggccg ggtcgcgggc gcaggtgtcg gccggggcgc
      841 gaagctggct cggcggggcg gggctcggct cggcgtccgg cggcgagagc cacagcgcga
      901 cggccccggc gagcgcggtg agccccgcgc ccgcgaacag gtatcgccgc tggctctggc
      961 gcagggggtc gcgcatcagc tcggcgagca gcgcgtccat gtcggcgaag cgccgctcgg
     1021 gcgccgctga cagtccgcgc aggacagtcc gtcgcaggtg cggcgggaac ttgcggctcg
     1081 cggcgaccga ctcgagcccg ccgcgcagca cctgcgggct gagcgtctgc cacgtgcgac
     1141 cgagcagcgg ccgccggccg tgcagcgcct cgaacagcgc gacgcaaaaa ctgtattgat
     1201 cggagcgggc gtcggcgggg cggccgaggt gcagctccgg ggccatgtac gccggggtgc
     1261 cgccgaggcc ggggtcggag cgcgacgcgt ccgacgtctc gtcgtcctcg gcggtatgtc
     1321 cgggcagcag gtcgctgatc gtcggcgccg cgaggccgaa gtcgatcacg cgggcgcggc
     1381 cgtcctcgcc gatgatcacg atctcgggcg tgaagtcgcg gtgcacgatg ccctcgcggt
     1441 gcgccgcggc gaggccctgc ccggccgcga ggaacacctc gcggaccgct cgccagtcac
     1501 ggggctgtcc cgagagccag gtgcgcaggt cgtcgccgcg gatgaactcc atggcgatga
     1561 agaggtggcc gtcgacgtcg ccgacgtcga acacgcgggc gacgttgggg tgcgagaggc
     1621 gggccatcgt catcgcctcg cggtgcaggc gggcgacggc ggcgcggtcc tccgtcggcg
     1681 gcacgacctt gagggcgacg cggcggtcga gctcggggtc gtgggcggcg tagacctccc
     1741 cgaagccgcc gcgtccgacc aggccgagca ccttgaagcg gccgatcatg cgtgcgccgt
     1801 cgtcctcgtc ggggacggga tccgcgggag acgacgcaaa cacggcggca atgtatcacg
     1861 cgaccctgcg cggggaatcc cgcgcctgat gcgcccgggc gcgggcgcca acgaccgcga
     1921 tcggcccgag gcgcagcgag gagcgcgcgg gtggcggggt ccggatgccg tacctgtccc
     1981 acgggacagg tgaaaggtct gcgggcccgc gacaagcatg ttggaccggg ctatcgtcgc
     2041 gccgtgcgga ccccggacac gggcggcaag ctgctgctcc tcagctcgct ctatctcgcg
     2101 cagggcctgc cggacgggtt ctttcgccag gcgctgccgg tgttgttgcg gttcgagggg
     2161 gtgtcgctcg agggcattgg actggcgtcg ctgctgatgt tgccgtgggc actgaagttc
     2221 ctgtgggcgc cgctggtcga ccgccacggc tggtcgcggt tcggtcgccg gcgcagctgg
     2281 atcgtcccgc tgcaggccct cgcggtcggg ctcttgctgc tgctgtcgga ggcggagcca
     2341 cagcgcggcc tcgcgccgct gctcgccggc gtcctggtga tcaacctcct ggcggcgacg
     2401 caggacatcg ctactgatgg cctcgcggtc acgatcctgc ggcccgagga gcgtggcctc
     2461 ggcaacggcg tgcaggtggc ggcctaccgg ctggggatga tcctgagtgg tggcgtcctc
     2521 atcatcttcc tcgaagagat cggctgggcc cgcacctttt atgtgatggc ggggatcagc
     2581 gcgctcgcgc tggtgcccct gctgatgaca cgcgagccgc cgtcgccccg gcccgagcgc
     2641 gcggggctgc gggtgctcgc cgacttcgtg cgccagccgg gcatgcgcgc gtggctgtgg
     2701 acgatcgccg cgttcaagct cggcgaggcg ttcggtgtgg cgatgctgcg gccgctgctc
     2761 gtcgaccgcg gcgtgtcgct gacgaccctc ggatggatca ccggcaccgc gggcttcatc
     2821 gccggcctcg cgggcgcgct gatcggcggc gcgatggtcg gcgtgtacgg ccgccatcgc
     2881 atgctgctga tcttcggcgt ggtccaggcg gtcgcggtcg ccagctacgt cgcgctggcg
     2941 atcgggccgc tcgacaacct gctcgtgtat ctgctgtgta cgctcgagca cctgagcagc
     3001 ggcatggcga ccgcggcgct gttcacgatg atgatggacg cggcccgccc gcaggcggca
     3061 ggcaccgact acacggcgca ggccagcgtc gtcgtgctct cgaccgggct cggcagcgcc
     3121 gtcagcgggt tctcggcgca ggcgctcggc tatgccggtc acttcatgct cgccgcgacg
     3181 ctgtgcctgc tcgcgctgat ccccgtggtg ttacacggca ggcgcgtgcg cgccgagctc
     3241 agccgcggtt gatcaagaag gtcgcggtgt cggagaagaa ggcgcgcagg gtcgcggcgc
     3301 tgtccggct
//
LOCUS       DASWNJ010001759         2356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_525106, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001759 DASWNJ010000000
VERSION     DASWNJ010001759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525106"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2356)
                     /locus_tag="VGB85_29420"
     CDS             complement(<1..>2356)
                     /locus_tag="VGB85_29420"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEY0138254.1"
                     /translation="GGIQDADVLRQISTAGLGQRGVVLDARLPVDIVASKLTRSTLPD
                     MKVTIPGAGWTWPDTLSGLQPGDEALVYADLPATAAMRVILTGEDRIDVTVPTTTVER
                     PLLERAWVGARIERLTSLRSALPASDSDTREAFRKQIIDLSTRFRVLSDFTALLVLET
                     DGDYDRFGIARGALTDILTVDGTGVAVQTRRSPPPSNGAPTLTPSGVTVEPTAEESLA
                     AAPGLAALQADPIADSPDSPKVAGDADAEDTYGGLLGTPPAAPGGLDGLGFTGTGVHA
                     GGGFSQRSLADTGLIGRGSGTGYGSGAGAGFGGRGGVPMVRQAKPEVVGALDKDIIRR
                     IVRAHINEIRYCYNRGLVRDPRLTGRVAMRFTIAGNGKVPLASVHESTLRDPAVGICI
                     TQAVKRWTFPRPEGGGSVNVTYPFVLAPSDGPVANTDPPAPELSPAEARELMRKAQAE
                     ARQAEREFAKWLREQADEERRMDAEERQEMADERRAELEAQRRDPLDGEMKEVMDLVK
                     ARKLAAALTRAAAWRGRDPGDVLALLALGEVHEAMNHPELAARAYGSIIDLFPSRADL
                     RRYAGARLERTGASALELAIDTFEKAVEQRPDHPASHRLYAFALSRAGRHEEALQAIL
                     TGLAAERPTGRFAGVIRVLREDATLIAAARVAADPASAAAVQAQLDPLGLVLASEPSL
                     RFVINWETDANDVDFHVHDGRGGHAYFQERALPSGGELFDDVTTGYGPECYAITGKAK
                     AYPYKLSAHYYDRGPMGYGMGKLQVIEHDGKGNLKFDERPFVIMV"
BASE COUNT          286 a          767 c          956 g          347 t
ORIGIN      
        1 accatgatga cgaagggtcg ctcgtcgaac ttgaggttgc ccttgccgtc gtgctcgatg
       61 acctgcagct tgcccatgcc gtagcccatc gggccgcggt cgtagtagtg ggcggagagc
      121 ttgtaggggt aggccttggc cttgccggtg atcgcgtagc actcggggcc gtagccggtg
      181 gtgacgtcgt cgaagagctc gccgccggac ggcagcgcgc gctcctggaa gtaggcgtgg
      241 ccgccgcggc cgtcgtggac gtggaagtcg acgtcgttgg cgtcggtctc ccagttgatg
      301 acgaagcgca gcgagggctc gctggcgagc acgaggccga gcgggtcgag ctgggcctgg
      361 accgcggcgg cgctggctgg atctgcggcg acgcgggcgg cggcgatcag ggtcgcgtcc
      421 tcgcgcagca cccggatgac gccggcgaag cgaccggtgg gacgctcggc ggcgaggccg
      481 gtgaggatcg cctgcagggc ctcctcgtgc cggccggcgc gggacagcgc gaaggcgtac
      541 agacgatggc tcgcggggtg gtcggggcgc tgctcgacgg ctttttcaaa ggtgtcgatc
      601 gccagctcga gggcgctggc gccggtgcgc tcgaggcggg ccccggcgta acgccgcaga
      661 tcggcgcggc tcgggaacag gtcgatgatc gacccgtacg cgcgggcggc cagctccggg
      721 tggttcatcg cctcgtggac ctcgccgagc gcgagcaggg cgagcacgtc gccggggtcc
      781 cggccgcgcc aggccgcggc acgggtcagc gccgcggcga gcttgcgtgc cttgaccagg
      841 tccatgacct ccttcatctc gccgtcgagc gggtcgcggc gctgcgcctc gagctccgcg
      901 cgccgctcgt cagccatctc ctggcgctcc tcggcgtcca tgcgccgctc ctcgtcggcc
      961 tgctcgcgga gccatttggc gaactcgcgc tcggcctggc gggcctcggc ctgggccttg
     1021 cgcatcagct cgcgcgcctc ggcggggctg agctcgggcg cgggcgggtc ggtgttggcg
     1081 acggggccgt cggagggcgc gagcacgaag ggataggtga cgttgaccga gccgccgccc
     1141 tcgggcctgg ggaaggtcca gcgcttcacg gcctgggtga tgcagatgcc gaccgcgggg
     1201 tcgcgcaggg tcgactcgtg cacggaggcg agggggacct tgccgttgcc ggcgatcgtg
     1261 aagcgcatgg cgacgcggcc ggtcagcctc gggtcgcgca cgaggccgcg gttgtagcag
     1321 tagcggatct cgttgatgtg ggcgcggacg atgcggcgaa tgatgtcctt gtcgagcgcg
     1381 ccgacgacct cgggcttggc ctgacgcacc atcggcacgc cgccacgacc gccgaagccc
     1441 gcgcccgcgc ccgagccgta gcctgtgccg ctgcctcgcc cgataaggcc ggtgtcggcg
     1501 aggctgcgct gactgaagcc accaccggca tgaaccccgg tgccggtgaa gccgaggccg
     1561 tcgaggccgc cgggcgcggc aggcggggtg ccgagcaggc cgccataagt gtcctcggcg
     1621 tcggcgtcgc cggcgacttt ggggctgtcg gggctgtcgg cgatcggatc ggcctgcagg
     1681 gcggcgaggc caggggcggc ggcgaggctc tcctcggcgg tcggctcgac cgtgaccccg
     1741 gagggcgtca gcgtgggcgc gccgttgctc ggcggagggc tgcggcgggt ctgtaccgcg
     1801 acgccggtgc cgtcgacggt gaggatgtcg gtgagcgcgc cgcgggcgat gccgaagcgg
     1861 tcgtagtcac cgtcggtctc gagcacgagc agcgccgtga agtcgctgag cacgcgaaag
     1921 cgggtcgaga ggtcgatgat ctgcttgcga aacgcctcgc gcgtgtcgct gtcgctcgcc
     1981 ggtagggccg agcgcaggct ggtcaggcgc tcgatgcggg cgccgaccca ggcgcgctcg
     2041 agcagcgggc gctcgacggt ggtggtcggc acggtgacgt cgatgcggtc ctcgccggtg
     2101 aggatcacgc gcatcgcggc ggtcgccggg aggtcggcgt agacgagcgc ctcgtcgccg
     2161 ggctggaggc cgctgagcgt gtcgggccag gtccaaccgg cgcccgggat cgtcaccttc
     2221 atgtccggca gggtgctgcg ggtcagcttg ctggcgacga tgtcgaccgg gaggcgggcg
     2281 tcgagcacga cgccgcgctg gccgagcccg gcggtggaga tctggcgcag cacgtcggcg
     2341 tcctggatcc cgccgt
//
LOCUS       DASWNJ010001760         2051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_414201, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001760 DASWNJ010000000
VERSION     DASWNJ010001760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414201"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..>2051)
                     /locus_tag="VGB85_29425"
     CDS             complement(224..>2051)
                     /locus_tag="VGB85_29425"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0138255.1"
                     /translation="PKTLEKLLAYHEERNGPIVEIENQLLELDLPTFYAEAIVHRYAD
                     QSLRMLREHPYRLAREVRGIGFTTADKIARALGVDLESDDRVDAGLIHTLEQAEQDGH
                     CALPQPVLIQKACGILDLAHDRIADGVSRLLASNELVLDDRPGLPAPLCYPPRHYEAE
                     LRVAATLVDLALGGGTRPRWRQIAMPTDLSEGQANAVAAIADAGLVVLTGGPGTGKST
                     VTRQIIETAIANGAELLLAAPTGRAAKRLAEATGHRATTIHRLLEIQGNTGQFGFDAG
                     NPLPPGLVVIDEASMLDLPLANSLLSALTPEHRLLLVGDADQLPSVGPGNVLRDVIAA
                     ADRPGSPIPVVRLTQIFRQAEGSSIVVGAHQILHGQVPQADRSADGQFYVIQARDAER
                     AHELVIKAVTERIPQAYGLDPRTDIQVLCPMHKGRAGTEAFNIALQAHHTAGRPELVQ
                     HLPGRAQPRIFRVGDRVMQIRNDHERGVFNGDIGVVVGVYPETQTLTVEIDGVPVSYD
                     SKQLGALQLAYAVSIHKSQGSEFPAVVVPLLGEHHVMLRRNLLYTAVTRARRLCLIVG
                     DSRALMQAVHRGDAARRYTGLSERLMAALRSQLGETEFVADE"
BASE COUNT          292 a          675 c          759 g          325 t
ORIGIN      
        1 ttcgtattcc aaactcgcag aactaaaccg ggtcgcctct gaatcaaatg atgagcggct
       61 tcggatcgct tccctagtct tccacgcaat ctataagggt cgacgtgtcg acatgcgaac
      121 cattttcaca tcctgagttc gtgaggacca ttccacgcta ccgcacttcg ccgacggtga
      181 gcaggacgag ctgcgttacg tgcagttgcg ctgcgcgtga tcgtcactcg tcggcgacga
      241 actcggtctc gccgagctgc gagcgcaggg cggccatcaa ccgctccgac aggccggtat
      301 agcgccgcgc cgcgtcgccg cggtggaccg cctgcatcag cgcccgcgag tcgccgacga
      361 tcaggcacag ccggcgcgcg cgcgtgaccg cggtgtacag caggttgcgg cgcagcatca
      421 cgtggtgctc gccgagcagc ggcacgacga cggccgggaa ctcgctgccc tgactcttat
      481 gaatggacac cgcgtaggcg agctgcagcg cgccgagctg cttgctgtcg taagagacag
      541 gtacgccgtc gatctcgacg gtgagcgtct gcgtctccgg gtacacgccg acgacgacgc
      601 cgatgtcgcc gttgaacacg ccacgctcgt ggtcgttgcg gatctgcatc acgcggtcgc
      661 cgacgcggaa gatccgcggc tgcgcgcgcc cgggcaggtg ctgcacgagc tcgggccggc
      721 cggcggtgtg gtgggcctgc agggcgatat tgaaggcctc ggtgccggcg cgacccttgt
      781 gcatcgggca cagcacctgg atgtcggtgc gcgggtcgag gccataggcc tgcgggatcc
      841 gctcggtgac ggccttgatc accagctcgt gcgcacgctc ggcgtcgcgg gcctggatca
      901 cgtagaactg gccgtcggcg ctgcggtcgg cctgcgggac ctgaccgtgc aggatctggt
      961 gcgcgccgac gacgatcgac gagccctccg cttgccggaa gatctgggtc agccgcacga
     1021 ccgggatcgg cgagccgggg cggtcggcgg cggcgatgac gtcgcgcagc acgttgccgg
     1081 gcccgaccga gggcagctga tcggcgtcgc cgacgagcag caggcggtgc tcgggtgtca
     1141 acgccgacag cagcgaattc gcgagcggca ggtcgagcat cgaggcctcg tcgatgacga
     1201 cgaggccggg cggcagcggg ttgccggcgt cgaagccaaa ctgcccggta ttgccctgga
     1261 tctcgagcag gcggtggatc gtggtcgcgc ggtggccggt cgcctcggcg aggcgcttgg
     1321 cggcgcggcc ggtcggcgcg gcgagcaaca gctccgcgcc gttggcgatc gcggtctcga
     1381 tgatctggcg ggtcaccgtg ctcttgccgg tgccgggtcc gccggtcagc acgacgaggc
     1441 ctgcgtcggc gatcgccgcg accgcgttgg cctggccctc gctgaggtcg gtgggcatgg
     1501 cgatctgccg ccagcgcgga cgcgtgccgc cgccgagcgc gaggtcgacg agcgtcgccg
     1561 cgacccgcag ctcggcctcg tagtgacgcg gcggataaca cagcggcgcg ggcaggcccg
     1621 ggcggtcgtc gagcacgagc tcgttgctgg cgagcagccg cgagacgccg tcggcgatcc
     1681 ggtcgtgcgc gaggtcgagg atcccgcagg ccttctggat cagcaccggc tgcggcagcg
     1741 cgcagtggcc gtcctgctcg gcctgctcga gcgtgtggat caggccggcg tcgacgcggt
     1801 cgtcgctctc gaggtcgacc ccgagcgcgc gcgcgatctt gtcggcggtc gtgaagccga
     1861 tgccccggac ctcgcgcgcg agccggtacg ggtgctctcg cagcatccgc agcgactggt
     1921 cggcgtagcg gtgcacgatc gcctccgcgt agaacgtcgg caggtcgagc tcgagcagct
     1981 ggttctcgat ctcgacgatc gggccgttgc gctcctcgtg gtaggcgagc aacttctcga
     2041 gggtcttggg g
//
LOCUS       DASWNJ010001761         1833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_102573, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001761 DASWNJ010000000
VERSION     DASWNJ010001761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102573"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VGB85_29430"
     CDS             complement(<1..271)
                     /locus_tag="VGB85_29430"
                     /inference="COORDINATES: protein motif:HMM:NF028290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEY0138256.1"
                     /translation="MRRLAVAPRRLPPAHFMSCLLAAAATCGPTVAEAAEAGGIDGRG
                     LGVAWALPFAGVLLSIALGPLLVPRLWHHHYGKLAAFWAACAGLAV"
     gene            467..769
                     /gene="clpS"
                     /locus_tag="VGB85_29435"
     CDS             467..769
                     /gene="clpS"
                     /locus_tag="VGB85_29435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEY0138257.1"
                     /translation="MDTPKGEIATKTRTKTQRPSLYKVLLLNDDYTPMEFVIVVLERF
                     FNKSGEDATRIMLHVHHNGVGVCGVYTYEVAETKVAQVVDFARQNQHPLQCTMEKE"
     gene            837..>1833
                     /locus_tag="VGB85_29440"
     CDS             837..>1833
                     /locus_tag="VGB85_29440"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0138258.1"
                     /translation="MPSASRTLEKTLRRALSLATSRQHEYATLEHLLMSLTEDQDALA
                     VLRACAVEIERLRATLMDFLDRELNGLVVDGAVDSKPTASFQRVIQRAAIHVQSAGRS
                     EVTGANVLVAIFSERESHAVYFLQEQSMSRLDAVNYMSHGVAKKPGMSEERRVRGADE
                     GGEGGGPGGGDKREGGNDALSAYCSNLNQKARDGRIDILVGRELEIERTIQILCRRSK
                     NNPLFVGDPGVGKTAIAEGLALRIVNGEVPEVLKAAVIFALDMGALLAGTRYRGDFEE
                     RLKAVLTELEQNKNAVLFIDEIHTVIGAGATSGGSLDASNILKPALASGDLRCIGS"
BASE COUNT          302 a          662 c          637 g          232 t
ORIGIN      
        1 ccacggccag ccccgcgcag gcggcccaga acgcggcgag cttgccgtag tggtggtgcc
       61 acaggcgggg caccaggagc gggccgagcg ctatcgagag caggacgccg gcgaacggca
      121 aggcccaggc caccccgagc ccgcgcccgt cgatgccgcc ggcctccgcc gcctccgcca
      181 ccgtcggccc gcaggttgcg gcggccgcga gcaggcacga catgaagtgc gccgggggca
      241 ggcgtcgcgg cgcgacggcg aggcgacgca tccgggctca agccttcttg acgttgcgcg
      301 ggaccgcccg gggtcatgcc ggccgccgcg ggccgggtca aggcgggcgg gtccgggccg
      361 gcgcagattc gcgtttgttg gccccggggt ttggactatg ttccggaggc cgcacggtgc
      421 agggacacac gaaccgacga tgagtgaccg gcttccgttc cggggcgtgg acacgcccaa
      481 gggcgagatc gcgaccaaga cccgtaccaa gacgcagcgc ccctcgctct acaaggtgct
      541 gctcctgaac gacgactaca cgccgatgga gttcgtcatc gtcgtcttgg agcgcttctt
      601 caacaagagc ggcgaggacg cgactcggat catgctgcac gtccaccaca acggggtcgg
      661 ggtctgcggc gtctacacct acgaggtggc cgagacgaag gtcgcccagg tggtggattt
      721 cgcccgccag aaccagcacc ccctgcaatg cacgatggag aaggagtgac cacgaccccc
      781 gccgccaccg ccgccacgcg ccgctccttc gcgtccgtcg agggagagcc caagtgatgc
      841 cgtccgcctc ccgcacgctc gagaagacgc tgcgccgggc cctgtcgctc gcgacctcgc
      901 gccagcacga gtacgcgacc ctcgagcacc tcctgatgtc gctcaccgag gaccaggacg
      961 cgctggccgt gctgcgcgcc tgcgccgtcg agatcgagcg cctgcgcgcg accctgatgg
     1021 acttcctcga ccgcgagctg aacgggctcg tcgtcgacgg cgccgtcgat tccaagccga
     1081 cggccagctt ccagcgggtc atccagcgcg ccgcgatcca cgtccagtcc gccggccgga
     1141 gcgaggtcac cggcgccaac gtgctggtgg cgatcttctc cgagcgcgag agccacgccg
     1201 tctacttcct gcaagagcag agcatgtcgc ggctcgacgc cgtgaactac atgtcgcacg
     1261 gcgtcgccaa gaagcccggc atgagcgagg agcggcgcgt gcgcggggcg gacgagggcg
     1321 gcgagggcgg cgggccgggc ggcggcgaca agcgcgaggg cggcaacgac gcgctctcgg
     1381 cctactgctc caacctgaac cagaaggccc gcgacggccg catcgacatc ctggtcggcc
     1441 gcgagctcga gatcgagcgc accatccaga tcctctgccg ccgctcgaag aacaacccgc
     1501 tgttcgtggg cgaccccggc gtcggcaaga ccgcgatcgc cgagggcttg gcgctcagga
     1561 tcgtcaacgg cgaggtgccc gaggtcctca aggccgcggt gatcttcgcg ctggacatgg
     1621 gcgcgctgct cgccggcacg cgctaccgcg gcgacttcga ggagcggctc aaggccgtgc
     1681 tcaccgagct ggagcagaac aagaacgccg tcctgttcat cgacgagatc cacaccgtca
     1741 tcggcgccgg cgccaccagc ggcggctcgc tcgacgcgtc gaacatcctc aagccggcgc
     1801 tcgccagcgg cgacctgcgc tgcatcggct cga
//
LOCUS       DASWNJ010001762         9815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_667805, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001762 DASWNJ010000000
VERSION     DASWNJ010001762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 9815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9815
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667805"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VGB85_29445"
     CDS             <1..533
                     /locus_tag="VGB85_29445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138259.1"
                     /translation="STGANYMSSASPALIDEYHCATELTQNGYMGMADECSGDNDDEQ
                     PANTAADAVSQPAVAAQNAGFMRDDAVLFVVAITDEDEQPVPGQSAQQIYDKLVAAKG
                     TIDNVVFLGIGGGSGCEGPYGSADDAQLLREVSELFAAQDRGLFWDLCQGDLQQAFAA
                     GLEVVDSACIAFDPPG"
     gene            complement(542..1447)
                     /locus_tag="VGB85_29450"
     CDS             complement(542..1447)
                     /locus_tag="VGB85_29450"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEY0138260.1"
                     /translation="MMLRRHAIPPVRSVLIYPREDGRARTTLIDAVDFLRREGVAVAV
                     PKDMGDGSEGAVNGATLVGAQALAGVDLIIALGGDGTLLRASRWAARAGIPVLGINLG
                     ELGFLTAYHRDEALVGLAAAVAGELSWEPRMRMYIEVRRGEQLLARETACNDAYIKHG
                     DVPRLLSLATEVAGQHMATYKADGLIISTPMGSTAYNLAAGGPIVAPGTQCLTITPIC
                     PHSLTHRPVVVSGESEIRITYAGPNDISAAFLTVDGQRSVELQLGDVVVATAADEPLK
                     LCPPATSVFRVLATKLGWNRGASDA"
     gene            complement(1454..2362)
                     /locus_tag="VGB85_29455"
     CDS             complement(1454..2362)
                     /locus_tag="VGB85_29455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0138261.1"
                     /translation="MTSAVKILIADDSASDRAAARGILSDAGYSVVEATDGQQVLDIF
                     QRERPAMVLLDVVMPKIGGLEVCRILKAKSSGYLPVLMVSTRNSVNARVEGLRSGADD
                     YLGKPYDSEELRLRVEVLLRTRAAFDGRPDSAADVQADAAPARVELPTEELHRRMQEE
                     FARAERYSDPLACLRIEVDGPAERAPALTEALRSVIETHVRKIDLVMKIDERGFLLVL
                     PNTHFPGALTVAERISRETRRPQRGEAQAGFTVSIGVSFFPNKDTHTLQDMLELVHAA
                     LDRARREQGGKICLFQHQGYLYAPDD"
     gene            2662..3099
                     /gene="rplM"
                     /locus_tag="VGB85_29460"
     CDS             2662..3099
                     /gene="rplM"
                     /locus_tag="VGB85_29460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY0138262.1"
                     /translation="MGTHYPNIADIRRDWLLVDLAGLTVGRAASQIANILRGKHKTTF
                     TPHMDVGDFVVCINAEKVVLSGTKLDDKLYRFNSGFPGGLKECSARNMLAKKPEEVIK
                     IAVRRMLPRSPLGRATYSKLKVYAGGEHPHDAQQPKPHTLPHS"
     gene            3109..3507
                     /gene="rpsI"
                     /locus_tag="VGB85_29465"
     CDS             3109..3507
                     /gene="rpsI"
                     /locus_tag="VGB85_29465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY0138263.1"
                     /translation="MAKDTMKFYATGRRKRAVARVWLFPGEGKIIINNRDADAYLARP
                     TNMKLIEQPLNATGTREKYDIWATCKGGGLSGQAGAVRLGIARALLRVAAEFRDTLKK
                     PGYLTRDARKVERKKYGRAGARARFQFSKR"
     gene            complement(3697..4191)
                     /locus_tag="VGB85_29470"
     CDS             complement(3697..4191)
                     /locus_tag="VGB85_29470"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY0138264.1"
                     /translation="MSRKLFVGGLSWNTTDDGLKEAFSRFGVVTEAKVVTDRETGRSR
                     GFGFVSYQEATDGAAAQQAMDGASLDGRSIRVNAAEDKPRTGGGGGGGGGGGRSFGGG
                     GGGGGGGGGGRGGGGGGGGFRDRDSGFGGDRGGGGGRGRGGGGGYDDDYGGGGGGRGR
                     GRGR"
     gene            4366..6396
                     /locus_tag="VGB85_29475"
     CDS             4366..6396
                     /locus_tag="VGB85_29475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HEY0138265.1"
                     /translation="MLYIPKHAVLETLGKPRLTELARSLGITPGRTKRDLQVQLAMLP
                     DRRFRDLLALLSEPELRAIARTHRLPQLKDPAALAESIALGPDDEAELLDPAADPETP
                     TPPEQGALPFKLRPGAPTLRIAGDSPPNDPGPDAPIPDNDDDLPANVTRLRRRKPASK
                     SGPEIAPPRRGELGFEATLWQAADKLRSNMDAAEYKHVALGLLFLKYVSDAFELRRAQ
                     LSAQGLPAEDPSAYVSPRAFFVPSTARWRAIEAASHEPRLGRTIDAAMAAIEAENPAL
                     RGVLPQIYQRPGLEGPLLGELVRLFADLGHADQRGRDLLGRIYEYFLTRFASAEGKNG
                     GQFYTPSSVVRLLVAMLAPTGGSVYDPACGSGGMFVQSDRFHVEHGGQHGALKIYGQE
                     SNPTTWRLARMNLALRGLDGDLGPEHADSFHRDLHPDLRADYVLANPPFNTKDWGAAR
                     LQDDPRWRFGVPPAQNANFAWVQHIIHHMAPRGLAGFVLANGAMTTSSAGEGEIRRAI
                     VEADLVDCMVALPAQLFYSTQIPVSLWLLARDKADPRLRDRRGQVLMIDARDLGGMVD
                     RTHRELQAPEIERLVGLYHAWRGDPVPGALKPIHLDVPGLCRSVGLEEIATLGHILAP
                     GRYVASAEPPAPLRPFADELADIDTILRAQLAQARELDDLLGELLDHLRPST"
     gene            6393..7742
                     /locus_tag="VGB85_29480"
     CDS             6393..7742
                     /locus_tag="VGB85_29480"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEY0138266.1"
                     /translation="MTALPPGWVTCTLDDIKAPGPHAIAGGPFGSDLGRADYVSHMPD
                     TPAVPVIRGSNLGAGEARFYTDDLVFIAAAKADQLARHSALPGDVVVTQRGTLEQVGL
                     VPAGTPWPRFILSQSQMKITVDPTRADPEYIYYWLLTPEIRDYVERHTIASGVPHTNL
                     ALFRQTPVRLPPLPQQRAIARALGILDARAALAQAMNDALDRLVRLLFRTWFIEHAPT
                     DSRARGELHGSTSFGESALFPATRAAPGPLGPLPTGWRAVPLTTCASFQNGTPFTGED
                     FGPAGRGKPVIKIGEIKRGITDQTRWAEDPPGARALDDGDLLMSWSGNPHTSIGTFIW
                     HGGPAWLNQHIFKVVPPAPRWRAFTYCLLRELQPRFVQLARSRQTTGLGHVSQKDMQT
                     LIVPRPSEALAEAFERRTAPAFARIAAHGQLQRTLARARAALLVKLTTGELPATRLP"
     gene            complement(7817..8230)
                     /locus_tag="VGB85_29485"
     CDS             complement(7817..8230)
                     /locus_tag="VGB85_29485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138267.1"
                     /translation="MRFDFTSSLASVCLALTLAACGEKTNETETDTDAVSTTASTTAT
                     TTDACAAGTAGCVDSTTTTDACDAGDAGCLPTTDACDAGTAGCNDTSSATDTDTAGDT
                     GGDITTDGCSGGGTAGDGGSTAGGEESGTAGGSCG"
     gene            complement(8279..>9815)
                     /locus_tag="VGB85_29490"
     CDS             complement(8279..>9815)
                     /locus_tag="VGB85_29490"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEY0138268.1"
                     /translation="QDPRVTVIVGGDEEVKYEAYSTGVAYGRLRLYGSDEVGGAAGSF
                     GWQDIVVLDAAPSDLSGVAAGVITGARQDVLSHLNVLSTQRGTPNAFVITPLKAFGQH
                     EGKLVRINVFEDYYTVNPAPLAEAEAYWAEHRPSVAVAHLPDPDAIAVQDVLEIPAAT
                     ASERALAVGRYGGKVTGLATLYPTLDAAYQTPALGIPTGNYIKFMQDNSWEVMLGGKL
                     QKLSFAATIDAWLADPTFRSDTGVRKAWLSALSAAMVQRGKVDPALLTDLRAKIGTSF
                     GDEGVMVRLRSSSNAEDALEFNGAGLYGSESACALDPDGSSGGDSACDPDKGRRPLDV
                     KLKTVWASLWSFGAFEERDYYQMDHRDVAMAVLVSTQYEDEQANGVAFTGNPIKPDDD
                     RYTINVQLGEIDVVSPPAGEIAELDRLTVEDGAVVQIERVAASSLVEPGQTVLSDAQL
                     KELGAVLAEISATYPVELGDHEASEVLLDLEFKITKEGQLIIKQIRTFLRSTLDPALP
                     SCL"
BASE COUNT         1425 a         3808 c         3157 g         1425 t
ORIGIN      
        1 actcgaccgg cgccaactac atgtcctcgg cctcgccggc gctgatcgac gagtaccact
       61 gcgccaccga gctgacgcag aacggctaca tgggcatggc cgacgagtgc tccggcgaca
      121 acgacgacga gcagccggcc aacaccgccg ccgacgccgt cagccagcct gcggtcgccg
      181 cccagaacgc cggcttcatg cgtgacgacg ccgtgctctt cgtcgtcgcg atcaccgacg
      241 aggacgagca gccggtcccc gggcagagcg cccagcagat ctacgacaag ctggtcgccg
      301 ccaagggcac gatcgacaac gtcgtgttcc tcggcatcgg cggcggcagc gggtgcgagg
      361 gcccctacgg cagcgccgac gacgcccagc tgctgcgcga ggtcagcgag ctgttcgccg
      421 cccaggaccg cggcctgttc tgggacctgt gccagggcga cctgcaacag gccttcgccg
      481 ccggcctcga ggtcgtcgac tccgcctgca tcgccttcga tccccccggc tgaagagatg
      541 atcacgcgtc cgaggcgccg cggttccagc cgagcttggt cgccagcacg cggaacacgc
      601 tcgtcgccgg cgggcacagc ttgagcggct cgtcggcggc ggtcgcgacg acgacgtcgc
      661 cgagctgcag ctcgacgctg cgctggccgt cgaccgtcag gaacgccgcg ctgatgtcgt
      721 tgggtcccgc gtaggtgatg cggatctccg actcgcccga gacgaccacc ggccggtgcg
      781 tgaggctgtg cgggcagatc ggcgtgatcg tcaggcactg cgtgccgggg gccacgatcg
      841 ggccgcccgc cgccaggttg taggccgtcg agcccatcgg cgtgctgatg atcaggccgt
      901 ccgccttgta ggtcgccatg tgctggcccg cgacctcggt cgccagcgac agcagccgcg
      961 gcacgtcgcc gtgcttgatg tacgcgtcgt tgcacgccgt ctcgcgcgcc agcaactgct
     1021 cgccgcgacg gacctcgatg tacatgcgca tccgcggctc ccacgacagc tcgccggcga
     1081 ccgccgccgc cagcccgacg agcgcctcgt cgcggtgata cgcggtcaaa aagccgagct
     1141 cgccgaggtt gatgccgagc accgggatgc ccgcgcgcgc cgcccagcga ctcgcgcgca
     1201 gcagcgtgcc gtcgccgccg agcgcgatga tcaggtcgac gcccgcgagc gcctgcgcgc
     1261 cgactaacgt cgccccgttg acggccccct cggagccgtc gcccatgtcc ttcgggacgg
     1321 cgaccgccac gccctcccgg cgcaggaagt cgaccgcgtc gatcagcgtg gtcctcgcgc
     1381 gtccgtcctc ccgtggatag atcagcacgg agcggacagg ggggatcgcg tggcggcgga
     1441 gcatcatggg ccctcaatcg tcgggggcgt agaggtaacc ctggtgctgg aacaggcaga
     1501 tcttcccgcc ttgctcgcgc cgggcccggt cgagcgcggc gtggaccagc tcgagcatgt
     1561 cctgcagcgt gtgcgtgtcc ttgttcggga agaaggacac cccgatcgac accgtgaagc
     1621 cggcctgggc ctcgccgcgc tgcggtcgtc gggtctcgcg ggagatccgc tcggccaccg
     1681 tcagcgcgcc ggggaagtgg gtgttcggca acaccagcaa gaagccgcgc tcgtcgatct
     1741 tcatcacgag gtcgatcttg cggacgtgcg tctcgatcac gcttcgcagc gcctccgtca
     1801 gcgcgggcgc ccgctcggcc gggccgtcga cctcgatgcg caggcacgcg agcgggtcgc
     1861 tgtagcgctc ggcccgggcg aactcctcct gcatgcgccg gtgcagctcc tcggtcggca
     1921 gctcgacccg cgccggcgca gcgtcggcct ggacgtcggc ggcgctgtct ggccggccgt
     1981 cgaaggcggc gcgcgtgcgc agcaggacct cgacgcgcag ccgcagctcc tcgctgtcgt
     2041 agggtttgcc gaggtagtcg tcggcgccgc tgcgcaggcc ctcgactctg gcgttgaccg
     2101 agttgcgcgt gctgaccatc aacaccggca ggtagccgct cgacttggcc ttgaggatcc
     2161 tgcacacctc gaggccgccg atcttcggca tcaccacgtc gagcagcacc atcgccgggc
     2221 gctcgcgttg aaagatgtcg aggacctgct ggccatcggt cgcctcgacg acgctgtacc
     2281 cagcatcact gaggatgccg cgcgccgcgg cccggtcgct ggccgagtcg tcggcgatga
     2341 ggatcttgac ggccgaggtc acgccggccc cagtatgtca cccttccagg gcggccgccc
     2401 agcgcgccag cggcaccgcc ccgggtccgg tcgatacccg ccgctctgcc cgccactctg
     2461 cccgcccctt ttcgcctcgc accacgcccg tcggagatcg ctcgtacccg tcatcgctca
     2521 caccgccggg gcgcggtcac ggcacccgtc aaacccggac gatccggccc gatcgacccg
     2581 ctccgaccgg ctttccccgg ttttccggcc ccggccgggc gcttgacagc cccagatggc
     2641 tcgcgtatcc atccgcttcc catgggtacc cactacccca acatcgcaga tatccgccgt
     2701 gactggctgc tggtcgacct cgctggtctg accgtcggcc gtgcagcgag ccagatcgcc
     2761 aacatcctcc gtggtaagca caagaccacc ttcaccccgc acatggatgt cggcgacttc
     2821 gtcgtctgca tcaacgcgga gaaggtcgtc ctgagcggca ccaagctcga tgacaagctg
     2881 taccgcttca actccgggtt ccccggcggc ttgaaggagt gcagcgcgcg caacatgctc
     2941 gccaagaagc ccgaagaggt gatcaagatc gccgttcgtc gtatgcttcc gcgctcgccc
     3001 ctcggccgcg ccacgtacag caagctcaag gtctacgccg gcggggagca cccccacgac
     3061 gcccagcagc ccaagcccca cacgctgccc cactcgtaat tcattcctat ggccaaagac
     3121 accatgaagt tttatgccac cggccgccgc aagcgcgcgg tcgcccgcgt gtggctgttc
     3181 cccggcgagg gcaagatcat catcaacaat cgtgacgcgg acgcctacct ggcccgcccc
     3241 acgaacatga agctgatcga gcagccgctc aacgcgaccg gcacccgcga gaagtacgac
     3301 atctgggcga cctgcaaggg tggcggtctg tccggccagg ccggcgccgt ccgcctcggc
     3361 atcgcccgcg ccctcctgcg cgtcgccgcc gagttccgcg acaccctcaa gaagcccggc
     3421 tacctgaccc gcgacgcccg caaggtcgag cgcaagaagt acggccgcgc cggcgcccgc
     3481 gcccgcttcc agttcagcaa gcgctgatcc gacggacctc cgggccccag caaaagagac
     3541 gctctccctc cggacagcgt ctcttttgcg ttggttcgct cgcaccgtcg agctgccgac
     3601 cgacccatcc aggaccgatc tccgggcgct gaaaaacccg gactcctggc ggggcccggg
     3661 tctcatacat cgagcgtccg ggacgcccgt gggatctcag cgaccgcgcc cgcgaccgcg
     3721 accaccaccg ccgccgccgt agtcgtcgtc gtagccgcca ccgccgccac gaccacggcc
     3781 accgccgccg ccgcgatcgc caccgaagcc ggagtcacgg tcgcggaacc caccgccacc
     3841 gccgccgcca ccacggccgc cgccgccacc accaccgccg ccgccgccgc cgccgaagct
     3901 acgaccgcct ccgccgccgc cgccaccacc gccaccggtg cggggcttgt cctcggccgc
     3961 gttgacgcgg atcgagcggc catcgagcga ggcgccgtcc atcgcctgct gggcggctgc
     4021 gccgtccgtg gcctcctggt aggacacgaa cccgaatcca cgcgagcgtc cggtctcgcg
     4081 gtccgtcacg accttggcct ccgtaaccac gccaaaccga ctgaaggcct ccttcaggcc
     4141 atcatccgtc gtgttccagc tcagaccacc aacaaagagt tttctcgaca ttccttcttc
     4201 gcttccgact ttcttgcgcg agactcgtgt gcacgccgac tggatcgtgc ctgttgacat
     4261 cgacgatgcg ccgcttcgcc ggacctgtca accgagcccg ggtcggttcg tgccggtgac
     4321 gtgtccgagg cgcggcaaat gtcgtaaaac cccggtccct cggccatgct ctacatcccc
     4381 aagcacgcgg tcctcgagac gctgggcaag ccccgcctca ccgagctggc ccgcagcctc
     4441 gggatcacgc ccggacgcac gaaaagagac ctccaggtcc agctggcgat gctccccgat
     4501 cgccgctttc gtgacctgct cgcgctgctc agcgagcccg agctgcgagc gatcgcccgc
     4561 acgcatcgcc tgccgcagct caaggacccg gcggccctcg ccgagtcgat cgccctcgga
     4621 cccgacgacg aggccgagct gctcgacccc gccgcggacc cggagacccc gacgccgccc
     4681 gaacagggcg cgctgccgtt caagctccgg cccggcgccc ccacgctgcg catcgcaggc
     4741 gacagccccc cgaacgatcc cggccccgac gcaccgatcc ccgacaacga cgacgacctg
     4801 cccgccaacg tcacgcgcct gcgtcgccgc aaacccgcca gcaagtccgg ccccgagatc
     4861 gcgcctccgc gccgcggcga gctcggcttc gaggccaccc tgtggcaggc cgccgacaag
     4921 ctgcgcagca acatggacgc cgccgagtac aagcacgtcg cgctcggcct gctgttcctc
     4981 aagtacgtct ccgacgcctt cgagctgcgc cgcgcccagc tgagcgcgca gggcctgccg
     5041 gccgaggacc cgtccgccta cgtcagcccg cgcgccttct tcgtgccctc gaccgcccgc
     5101 tggcgcgcga tcgaggccgc cagccacgag ccgaggctcg gccgcacgat cgacgcggcg
     5161 atggccgcga tcgaggccga gaaccccgcc ctgcgcggcg tgctcccgca gatctatcaa
     5221 cgccccgggc tcgagggccc gctgctcggc gagctcgtgc gcctgttcgc cgacctcggc
     5281 cacgccgacc agcgcggccg cgatctcctc ggccggatct acgagtactt cctgacccgc
     5341 ttcgcctccg ccgaggggaa aaacggcggc cagttctaca cgccgagctc cgtcgtgcgc
     5401 ttgctggtcg cgatgctcgc gccgaccggc ggcagcgtct acgaccctgc ctgcggctcg
     5461 ggcggcatgt tcgtgcagag cgaccgcttc cacgtcgagc acggcggcca gcacggcgcc
     5521 ctcaagatct acggccagga gtccaacccg accacctggc ggctcgctcg catgaacctc
     5581 gcgctgcgcg gcctcgacgg cgacctcggc cccgagcacg ccgacagctt ccaccgcgac
     5641 ctgcaccccg acctgcgcgc cgactacgtg ctcgccaacc cgccgttcaa caccaaggac
     5701 tggggcgccg cccgcctgca ggacgacccg cgctggcgct tcggcgtgcc gccggcgcaa
     5761 aacgccaact tcgcgtgggt ccagcacatc atccaccaca tggcgccgcg cggcctggcc
     5821 ggcttcgtgc tcgccaacgg ggcgatgacg acgagcagcg ccggcgaggg ggagatccgc
     5881 cgcgcgatcg tcgaggccga cctcgtcgac tgcatggtcg cgctcccggc ccagctgttc
     5941 tactcgacgc agatccccgt gtcgttgtgg ctgctcgcgc gcgacaaggc cgacccgcgc
     6001 ctgcgcgacc gccggggcca ggtcctgatg atcgacgccc gcgacctcgg cggcatggtc
     6061 gaccgcaccc accgcgaatt gcaggccccg gagatcgagc gcctcgtcgg cctgtaccac
     6121 gcctggcgcg gcgaccccgt gcccggcgcc ctcaagccga tccacctcga cgtgcccggc
     6181 ctgtgccgat ccgtcggcct cgaggagatc gccacgctcg gccacatcct cgcgcccggc
     6241 cgctacgtcg cctccgccga gccgcccgcg ccgctgcgcc ccttcgccga cgagctcgcc
     6301 gacatcgaca ccatcctgcg cgcccagctc gcgcaggcgc gtgagctcga cgacctgctc
     6361 ggcgagctcc tcgaccacct ccgtccctcg acatgaccgc gttgcccccc ggctgggtca
     6421 cctgcaccct cgacgacatc aaggcccccg gaccccacgc gatcgccggc ggcccgttcg
     6481 gctcggacct cggccgcgcc gactatgtgt ctcatatgcc cgacacgccg gccgtgccgg
     6541 tgatccgcgg ctcgaacctc ggcgccggcg aggcccgctt ctacaccgat gacctcgtgt
     6601 tcatcgccgc cgccaaggcc gaccagctcg ccagacacag cgcgctgccg ggcgacgtcg
     6661 tcgtcaccca gcgcggcacg ctcgagcagg tcggcctggt ccccgcgggc accccgtggc
     6721 cgcgcttcat cctgtcgcag agccagatga agatcaccgt cgacccgacg cgcgccgacc
     6781 ccgagtacat ttattactgg ctgctgaccc cggagatccg cgactacgtc gagcgccaca
     6841 cgatcgcctc cggggtcccg cacaccaacc tggcgctgtt ccgccagacg ccggtgcgcc
     6901 tgcccccgct tccccagcaa cgcgcgatcg cccgcgccct cgggatcctc gacgcccgcg
     6961 ccgcgctcgc gcaggcgatg aacgacgcgc tcgatcgcct ggtccgcctg ctctttcgca
     7021 catggttcat cgaacatgcc ccgacggaca gccgcgcccg cggcgagctg cacggaagca
     7081 ccagcttcgg cgagtcggcc ctgttcccgg cgacgcgcgc ggcccccggc ccgctcggcc
     7141 cgctgccgac cggctggcgc gccgtgccgc tgacgacctg cgcgagcttt caaaacggca
     7201 cccccttcac cggcgaggac ttcggtcccg cgggtcgcgg caagccggtg atcaagatcg
     7261 gcgagatcaa gcgcggcatc accgatcaga cccgctgggc cgaggacccg cccggcgccc
     7321 gcgcgctcga cgacggcgac ctgctgatgt cgtggtccgg caacccgcac acttcgatcg
     7381 gcaccttcat ctggcacggc ggcccggcct ggctcaacca gcacattttt aaagtggtgc
     7441 cacccgcccc acgctggcgc gccttcacct actgtctcct gcgcgagctc cagccgcgct
     7501 tcgtccagct cgctcgcagc cgccagacca ccggcctcgg ccacgtcagc caaaaggaca
     7561 tgcagacgct gatcgtgcca cgcccgtccg aagccctcgc cgaggccttc gagcgccgca
     7621 ccgccccggc cttcgcccgc atcgccgccc acggccagct ccagcgcacc ctcgcacgcg
     7681 cccgcgctgc cctgctggtc aagctgacga ccggcgagct gcccgcgacc cgcttgccct
     7741 gaacgtgctt cgatgctcgc gaaaacgcgg acccgggctc gccgggtccg cgtgatgttg
     7801 gagttacgct cgggtttcag ccgcaggagc cgccggccgt gccgctctct tcgccgccgg
     7861 ccgtgctgcc gccgtcaccg gccgtgccgc cgccgctgca gccgtcggtc gtgatgtcgc
     7921 cgccggtgtc gccggccgtg tcggtgtcgg tggcgctgct ggtgtcgttg cagccggccg
     7981 tgcccgcgtc gcacgcgtcg gtcgtcggga ggcagccggc atcgcccgcg tcgcaggcgt
     8041 cggtcgtcgt cgtcgagtcg acgcagccgg ccgtgcccgc ggcgcaggcg tcggtggtcg
     8101 tggcggtggt cgaagcggtg gtggagaccg cgtcggtgtc ggtctcggtc tcgttggtct
     8161 tttcgccgca ggccgcgagg gtcagcgcga ggcacacaga agcgagcgat gaagtgaagt
     8221 caaaacgcat cggcggtgct taacagatcg cgtgccgcac cgcaacgaaa cgctgcgctc
     8281 acaaacagct cggcagcgcg ggatcgaggg tcgagcgcaa gaaggtgcgg atctgcttga
     8341 tgatcagctg accttccttc gtgatcttga actcgaggtc cagcagcacc tcgctcgctt
     8401 cgtgatcgcc gagctcgacc ggataggtcg ccgagatctc cgcgagcacc gcaccgagct
     8461 ccttgagctg cgcatcactg agcacggtct gtccgggctc gacgagcgac gacgcggcga
     8521 cacgctcgat ctgcaccacg gcgccgtcct cgacggtcag gcggtcgagc tcggcgatct
     8581 cacctgccgg cgggctgacg acgtcgatct cgccgagctg cacgttgatc gtgtagcggt
     8641 cgtcgtccgg cttgatcggg ttgccggtga acgcgacgcc gttggcctgc tcgtcctcgt
     8701 actgcgtgct gaccagcacg gccatcgcga cgtcgcggtg gtccatctga tagtagtcgc
     8761 gctcctcgaa cgcgccgaag ctccacaggc tcgcccacac cgtcttgagc ttcacgtcga
     8821 gcggccggcg gcccttgtcc ggatcgcagg cggagtcacc gccgctgctg ccgtccgggt
     8881 cgagcgcgca cgcgctctcc gagccgtaga gcccggcgcc gttgaactcg agcgcgtcct
     8941 cggcgttcga ggacgagcgc aggcggacca tcacgccctc gtcgccgaag ctcgtgccga
     9001 tcttcgcgcg caggtccgtg agcagggccg ggtcgacctt gccgcgctgg accatcgcgg
     9061 cgctgagggc cgacagccac gccttgcgca cgccggtgtc cgagcgaaag gtcgggtcgg
     9121 cgagccaggc gtcgatcgtc gcggcgaagc tcagcttctg cagcttgccg ccgagcatca
     9181 cctcccagct gttgtcctgc atgaacttga tgtagttgcc ggtcgggatg ccgagcgcgg
     9241 gggtctggta ggccgcgtcg agcgtcgggt acagggtggc gagcccggtg accttgccgc
     9301 cgtagcgccc gaccgcgagc gcacgctcgc tggcggtcgc cgccgggatc tcgagcacgt
     9361 cctgcaccgc gatcgcgtcg gggtcgggca ggtgcgcgac ggcgacgctc gggcggtgct
     9421 cggcccagta cgcctcggcc tcggcgagcg gggccgggtt caccgtgtag tagtcctcga
     9481 agacgttgat gcgcaccagc ttgccctcgt gctggccgaa ggccttgagc ggcgtgatga
     9541 cgaaggcgtt gggggtgccg cgctgggtcg agagcacgtt gaggtggctg agcacgtcct
     9601 ggcgcgcgcc ggtgatcacg ccggccgcga cgccgctgag gtcgctcggc gccgcgtcga
     9661 gcacgacgat gtcctgccag ccgaagctgc ccgccgcgcc gccgacctca tcggagccgt
     9721 acaggcggag cctcccgtag gcgacgccgg tcgagtaggc ctcgtacttg acctcctcgt
     9781 cgccgccgac gatcaccgtc acccgcgggt cctgc
//
LOCUS       DASWNJ010001763         2971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_525227, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001763 DASWNJ010000000
VERSION     DASWNJ010001763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525227"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VGB85_29495"
     CDS             <1..877
                     /locus_tag="VGB85_29495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138269.1"
                     /translation="FVMRTLFLPLCLLAPTLMACDEEGDIPFIDDEEELESRFFAGVN
                     GDACLASPYNCKLRVSGGNRVATNDPDDLNAWGLVKNVPVRDGNGDIMITSTKSSTTF
                     NYGQSRTFAGETYVFAVSTSNSSAGWMPLSSILGKSSFESKLGKVSALGAGLAKLGCY
                     QVKDSHDATLELKKVVYDSKVTHERAGDYLPLPRANGKRSANLTFNVPGFALGGVAVD
                     HFPAGTKFQRLDVPTDSGAPSIDIPLYVKDSEGRYRKQSGTMKFLYGYVIAKTGTKRN
                     GWMAYDALSVSSGCP"
     gene            complement(896..1948)
                     /locus_tag="VGB85_29500"
     CDS             complement(896..1948)
                     /locus_tag="VGB85_29500"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0138270.1"
                     /translation="MTRRDPLLPKIASAATRMLFRLPASWLARLAGPRPPGDLLDPQT
                     AVLVRITNALRLDTLKGLSPAVARARTASSLRLLAGRRHPDVEVDERSFAGPGGPLTL
                     RVYTPKDIARPAPALLYLHGGGFVIGSLDTHDAPCSLFAALGRCVVLSLDYRLAPEHK
                     FPAAVDDCLAAFRWLADNAAELGLDPRRLAVGGDSAGGNLAAVISQTAVREAWPVWPA
                     FQLLIYPALDMRRQHASHREFRDGYILTSDSLDFYLGHYLRTPEDVTDLRVSPLLAAD
                     LSRLPPAYITTAGFDPLRDEGDEYAERLRRDGVPAELARHTGLVHGFVNLGAGLQAAA
                     AALTDAGRALRDALHR"
     gene            complement(2073..2651)
                     /locus_tag="VGB85_29505"
     CDS             complement(2073..2651)
                     /locus_tag="VGB85_29505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138271.1"
                     /translation="MTPASRARLVVPLLAALLALVACRPEPAATTTAPPTPAPVSALA
                     GFDGPVEDDPVAKEATALSEGPLTQCAEKDAAGMQMEGDLIASKLAPGQVLEQEFRLQ
                     PGRCYTLIAVGGEGIKELDATFETIGPVRSKSTVLASDALSGTTAVVGGGEACFAWKP
                     ESNATPAKFVLRVRGGDGVVVSQLYARDATTP"
     gene            complement(2745..>2971)
                     /locus_tag="VGB85_29510"
     CDS             complement(2745..>2971)
                     /locus_tag="VGB85_29510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138272.1"
                     /translation="GATTLAQVLAREHADDPRALRFNALCPGWVKTAMGGEGAEREVS
                     EGADTAVWLATQPPGGPTGGFFRDRAPASW"
BASE COUNT          452 a          968 c         1146 g          405 t
ORIGIN      
        1 gttcgtgatg cgcacgctgt tcttgccgct ctgtctgctc gctcccacgc tcatggcctg
       61 tgacgaggag ggtgacatcc ccttcatcga cgacgaggag gagctggagt cccgcttctt
      121 cgccggcgtc aacggcgacg cctgtctggc gagcccgtac aattgcaagc tgcgggtctc
      181 cgggggcaac cgcgtggcga cgaacgaccc ggacgacctc aacgcgtggg gcctggtcaa
      241 gaacgtgccc gtgcgcgacg gcaacggcga catcatgatc acgtcgacca agtcgagcac
      301 gaccttcaac tatggccagt cgcggacctt tgccggcgag acctacgtgt tcgcggtgtc
      361 gacctccaac agcagcgccg ggtggatgcc gctgtcgagc attttgggca agtcgtcgtt
      421 cgagagcaag ctcggcaagg tgtcggcgct cggggccggg ctggccaagc tcgggtgtta
      481 tcaggtcaag gactcgcacg acgccaccct cgaattgaag aaggtggtgt acgacagcaa
      541 ggtgacgcac gagcgggcgg gtgactatct cccgctgccg cgcgccaacg gcaagcgctc
      601 ggccaacctg accttcaatg ttccgggctt cgcgctcggc ggcgtcgcgg tcgaccactt
      661 cccggccggc accaagttcc agcgcctcga cgtgccgacc gacagcggcg cgccgtcgat
      721 cgacatcccg ctgtacgtca aggacagcga gggccgctac cgcaagcagt cgggcacgat
      781 gaagttcctg tacggctacg tgatcgccaa gaccggcacc aagcgcaacg gctggatggc
      841 ctacgacgcg ctgtcggtca gcagcggctg cccgtgacct gtcctcgcgg acagctcagc
      901 ggtgcagcgc gtcgcgcagg gcccggccgg cgtccgtgag cgcggcggcg gccgcctgga
      961 ggccggcgcc gaggttgacg aagccgtgca cgaggccggt gtggcgggcc agctcggccg
     1021 gcacgccgtc gcggcgcagg cgctcggcgt actcgtcacc ctcgtcgcgc agcgggtcga
     1081 agccggcggt ggtgatgtac gcgggcggca ggcgcgagag gtccgcggcg agcagcggcg
     1141 agacgcgcag gtcggtgacg tcctccggcg tgcggagata gtggccgagg tagaagtcga
     1201 gggagtcgga ggtcaggatg tagccgtcgc gaaactcgcg gtgcgaggcg tgctggcggc
     1261 gcatgtcgag cgccggatag atcagcagct ggaacgcggg ccacaccggc caggcctcgc
     1321 gcacggccgt ctgcgagatc acagcggcga ggttgccacc ggcgctgtcg cccccgacgg
     1381 cgaggcggcg cgggtcgagg ccgagctcgg cggcgttgtc ggcgagccag cgaaacgccg
     1441 cgaggcagtc gtcgacggcg gccgggaact tgtgctcggg cgcgaggcga tagtcgagcg
     1501 agagcacgac gcagcgaccg agcgcggcga acagcgagca gggcgcgtcg tgggtgtcga
     1561 ggctgccgat cacgaagccg ccgccgtgca agtacaacag ggcaggcgcg ggccgggcga
     1621 tgtcctttgg ggtgtagacc cgcagcgtca gcggtccgcc gggtccggcg aagctgcgct
     1681 cgtcgacctc gacgtccggg tgccggcgac cggcgagcag gcgcagcgaa ctggcggtgc
     1741 gggcccgggc gacggccggt gagaggccct tgagggtgtc gaggcgcagg gcgttggtga
     1801 tacgaacgag gacggcggtc tgcggatcga gcaggtcacc ggggggccgt ggaccggcga
     1861 gccgggccag ccacgacgcg ggcaaccgga acagcatacg tgtggcggcc gaggcgatct
     1921 tcgggaggag cgggtcgcgg cgggtcatgg cgagggcagg gtaacagctt tcgcggaccg
     1981 gccgggtcag cgatgtcttg tggccgcgcg tggcagggcg aacggcgatg gtcgtgatga
     2041 tcgttcgcgc cgtactggcg gcgccgtgaa cgtcagggcg tggtcgcgtc gcgggcgtag
     2101 agctggctga cgacgacacc gtcgccgccg cgcacgcgca gcacgaactt ggcgggggtc
     2161 gcgttgctct cgggcttcca ggcgaagcag gcctcgccgc cgccgacgac cgcggtcgtg
     2221 ccggacaggg cgtcggaggc gagcacggtc gacttgctgc gcaccgggcc gatcgtctcg
     2281 aaggtggcgt cgagctcctt gatgccctca ccaccgacgg cgatcagcgt gtaacagcgg
     2341 ccgggctgca ggcgaaactc ctgctcgagc acctggcccg gcgcgagctt gctggcgatc
     2401 aggtcgccct ccatctgcat gccggcggcg tctttctcgg cgcactgggt cagcgggccc
     2461 tcggataatg cggtcgcttc cttggcgacc gggtcgtcct cgaccggacc gtcgaagccg
     2521 gcgagcgccg agacgggcgc tggagtcggt ggcgcggtcg tcgtcgccgc gggctcggga
     2581 cggcacgcca cgagcgcgag caaggcggcg agcaggggga ccacgaggcg tgcgcgcgag
     2641 gcaggggtca agacggctct ccgtgcgcag gataggccgg cgcggcatca tggcgcaagc
     2701 gcggcgctcg ctcgccagtt gctcatcacg cgctcaccac gagctcacca tgaagcgggc
     2761 gcgcggtcgc gaaagaagcc gccggtcggg ccgccgggcg gctgcgtggc cagccagacg
     2821 gcggtgtcgg ccccctcgga gacctcgcgc tcggcaccct cgccgcccat cgcggtcttc
     2881 acccagccgg ggcacagcgc gttgaagcgg agcgcgcgag ggtcgtccgc gtgctcgcgc
     2941 gcgagcacct gcgcgagcgt ggtcgcgccg a
//
LOCUS       DASWNJ010001764         2890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_66822, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001764 DASWNJ010000000
VERSION     DASWNJ010001764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66822"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1250)
                     /locus_tag="VGB85_29515"
     CDS             complement(30..1250)
                     /locus_tag="VGB85_29515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0138273.1"
                     /translation="MNYIPFILVIALGCTPKSLGLGDTDDAGDSGDSSGPATSGDPTT
                     GTPGSDGLAVVEGIGGSDLAVGADGSIVVIGQSGYASDPYVNYNLATEGFQNDWIGKF
                     DAAGAPIWVHETPSANPGGSPFAVAVAIGPDGGIHVAWTDSLHKLDPAGKTLWTRPLS
                     DFPRDLAVTADGRTVVASEVYIDLADMPLVQIFAPDGAPLVEQAYDRPEYHEPGIGDV
                     HVHALALDEGDGTVVLAGENSTQAWLLRADLESGALLWHQPITEAMGYGQVGDIGISA
                     DGTILALVGDHGTKVRAYSRAGELLWSFVPTVVERPNSLAVAADGSFALTDAVILPGI
                     HPLERCSSFECPERTRVERRAADRSLMWAASNDECKEGRVVAVTPGDGVVVLGQCSKP
                     PYGPYSIGLLQYAL"
     gene            complement(1323..1994)
                     /locus_tag="VGB85_29520"
     CDS             complement(1323..1994)
                     /locus_tag="VGB85_29520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138274.1"
                     /translation="MEPLDPELRRIIDEGLRQAAPGPEVEARALAGLLARISEAGQTS
                     DTSDPGRDPTPLVSATKASALPGGLKALILATVIAGGGITIAAVTRDPPAPAVVTARP
                     PPAPVVAPVKLETSIAAPPASRPVRVVRPAPDTLLAETRALAEADAALASGRHDLALT
                     LAAKIARQHPKGQLVIERVAIEVSARCGLHDPGAGAGAREFLRELGDTAVAAKVRTRC
                     AEVLR"
     gene            complement(1981..2526)
                     /locus_tag="VGB85_29525"
     CDS             complement(1981..2526)
                     /locus_tag="VGB85_29525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY0138275.1"
                     /translation="MSSEPPGGGVPWIVDAYRQHFRYVYALVGRLGVPSAGVEDVAQE
                     VFLVLHRRRAEFRGDSSVRTWLHGITVHVARRHRDAIRRRSERTAGLDEPPPPIPPDV
                     ELDHKQALRALDRLLDTLADEQREVFVLAEVAGCSAPEIAEALGLNINTVYSRLRLAR
                     GRLQGALAALRAEKGGKYGTA"
     gene            complement(2559..>2890)
                     /locus_tag="VGB85_29530"
     CDS             complement(2559..>2890)
                     /locus_tag="VGB85_29530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138276.1"
                     /translation="LGVLACVAPGPARVRVPLCLGVEAGPLICRGLGVPTTRLRADLW
                     ASGLAGAAVVGRVHPRVAVVVGAELSLGLRRPAFHVGAREELTRAGAVGFRALAGLEF
                     SLNLAGP"
BASE COUNT          410 a         1028 c         1063 g          389 t
ORIGIN      
        1 ccggaccccg cggcatcatc gccggtcgct cacagcgcgt actgcaacag gccgatcgag
       61 tacgggccgt acggcggctt ggagcactgg ccgagcacca cgacgccgtc gcccggcgtc
      121 accgcgacga cgcgaccctc cttgcactcg tcgttgctgg ccgcccacat cagcgagcgg
      181 tcggccgcgc gccgctcgac ccgcgtgcgc tccgggcact cgaacgacga gcagcgctcg
      241 agcgggtgga tcccggggag gatcacggcg tcggtcagcg cgaacgaccc gtccgcggcg
      301 acggcgagcg agtttgggcg ctcgacgacc gtcggcacga agctccacag cagctcaccg
      361 gcgcgggagt aggcccgcac cttggtcccg tgatcgccga cgagcgccag gatcgtgccg
      421 tcggcggaga tcccgatgtc gccgacctgg ccgtacccca tggcctcggt gatcggctgg
      481 tgccacagca gcgcaccact ctcgaggtcc gcgcgcagca gccacgcctg cgtggagttc
      541 tcgcccgcga gcaccacggt cccgtcaccc tcgtcgagcg cgagcgcgtg cacgtgcacg
      601 tcgccgatcc cgggttcgtg gtactcgggg cgatcgtagg cctgctcgac gagcggcgcg
      661 ccgtccgggg cgaagatctg gaccagcggc atgtcggcga gatcgatgta tacctcgctg
      721 gcgacgaccg tgcgcccgtc ggcggtcacg gccaagtccc gggggaagtc ggacaggggc
      781 cgggtccaca gcgtctttcc agctggatcg agcttgtgca ggctgtcggt ccacgcgacg
      841 tggatcccac cgtccgggcc gatcgccacc gcgaccgcga acggcgagcc gcccggattg
      901 gcgctcggcg tctcgtgcac ccatatcggc gcgcccgcgg cgtcgaactt gccgatccag
      961 tcgttttgaa acccctcggt cgcgaggttg taattgacgt agggatcgct ggcatagccg
     1021 ctctgaccaa tgacgacgat cgacccgtcg gcgccgacgg cgaggtcgct gccgccgata
     1081 ccctcgacga ccgcgagacc gtcggagccc ggcgtaccgg tcgtcggatc gccgctggtg
     1141 gcagggccgc tggagtcgcc gctgtccccg gcatcatcgg tgtccccgag gccgagagat
     1201 ttgggggtgc agccgagcgc gatcacgagg atgaatggga tgtagttcat gcggtgcata
     1261 tctcttggtc ccgtcagccg ccgcgatccg tcacgggccg atgctttttt gaattgtaaa
     1321 atttatcgta ggacctcggc gcagcgggtc cggaccttgg ccgcgacggc ggtgtcgccg
     1381 agctcgcgca ggaactcgcg ggcgcccgca ccggcgccgg ggtcgtgaag accgcagcgg
     1441 gcgctgacct cgatcgccac gcgctcgatc accagctgac cttttgggtg ctggcgggcg
     1501 atcttcgcgg cgagcgtgag ggcgaggtcg tggcgcccgc tggcgagcgc ggcgtcggcc
     1561 tcggcgagcg cgcgggtctc ggcgagcagg gtgtccggcg cggggcgaac cacgcggacc
     1621 gggcgcgacg ccggcggtgc tgcgatcgac gtctcgagct tcaccggcgc gacgacgggc
     1681 gcgggcggtg gcctggcggt cacgaccgcg ggcgcgggcg gatctctggt gactgccgcg
     1741 atcgtgatcc cgccgcccgc gatcacggtc gccaggatca gcgccttgag gccgccgggc
     1801 agcgcgctcg ccttcgtcgc cgagaccagc ggcgtcggat cgcggccagg gtcggaagta
     1861 tcgctggtct gaccggcctc ggagatccgc gcgagtaatc ccgcgagcgc gcgcgcctcg
     1921 acctcgggac ccggcgcggc ctggcgcagg ccctcgtcaa tgatgcggcg cagctccggg
     1981 tcaagcggtt ccatatttgc ctcctttctc ggcgcgcagg gcagccagcg cgccttgaag
     2041 gcggccccgc gcgaggcgca gccgcgaata tacggtgttg atattgagcc cgagcgcctc
     2101 ggcgatctcg ggcgcgctgc agcccgcgac ctcggcgagc acgaacacct cgcgctgctc
     2161 gtcggcgagg gtgtcgagca ggcgatcgag ggcgcgcagg gcctgtttgt ggtcgagctc
     2221 gacgtcgggc ggtatcggcg gtggcggctc gtcgaggccg gcggtccgct cgctgcggcg
     2281 acggatcgcg tcgcggtgcc gacgagcgac gtgcacggtg atcccgtgca gccaggtgcg
     2341 gaccgaggag tcgccgcgaa actcggcccg gcgccggtgc aggaccagga acacctcctg
     2401 cgcgacgtcc tcgacgccgg ccgacgggac cccgagccgg ccgacgagcg catacacgta
     2461 gcggaagtgc tggcggtacg cgtcgacgat ccaggggacg ccgccgcccg ggggctcgct
     2521 gctcacccgg tcactgtccc gcgagcgcgg ccgatccgtc acggcccggc gagatttaaa
     2581 gaaaattcga gcccggccag ggcccgaaac ccgaccgccc cggcgcgcgt cagctcctcg
     2641 cgcgcgccga cgtgaaaggc ggggcgccgc agcccgaggg acagctcggc gccgacgacc
     2701 acggcgaccc gcgggtgcac acggccgacc accgcggcgc cggcgagccc cgaggcccac
     2761 aggtcggcgc gcagccgcgt ggtgggcacg ccgaggccgc gacatatcag cggtccggcc
     2821 tcgacgccga ggcacagcgg cacccgcacg cgcgccgggc cgggcgccac gcaggcgagc
     2881 acgccgagcg
//
LOCUS       DASWNJ010001765         2474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_66844, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001765 DASWNJ010000000
VERSION     DASWNJ010001765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66844"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2474
                     /locus_tag="VGB85_29535"
     CDS             <1..>2474
                     /locus_tag="VGB85_29535"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEY0138277.1"
                     /translation="AAAVRDAHEPIAIVGASCRLPGDVDSPRAFWSLMDGRVDAITEV
                     PGERWDIDEWYDADFHAPGKMNTRYGGFVTGMTLFDAEFFGITPREASVLDPQQRMFL
                     ELSWEALEHAGIAPSSLQGTETGVYVGLMSHDYTLLQWGDFTRFDGSIGIGNTGSAAA
                     GRVSYTLGTHGPSMSIDTACSSSLVATHLACNALRNHECDVALAGGVSLNLHPAIFVE
                     FSRLRGMAPDGRCKTFSAAADGVAWAEGCGVLVLKRLSDARRDGDRVLALLRGSAVNH
                     DGRSNGLTAPSGPAQEAVVRRALRDAGVKPSDIDYVESHGTGTPIGDPIEARALGAVF
                     RGPRASGRPLWLGAVKTNIGHPQAAAGVAGILKVALALRNRRIPANLHFEAPSPNIQW
                     DEFSLAIPVAPVAWERGERPRIAGVSAFGISGINAHVVVQEAPEAADEDEPASESVHT
                     HHLLPLAAKSPAALAALVARYLAHLEANPGQGLQDICHTAAVGRDHPAHRLAIVSEDL
                     ATLRETLLAVVDQQERANVVVSPELRKSGAAIVFVFPGQGSQWLGMGRELMREPAFAE
                     AIAACDAAIAEEAGWSLRDELAADEAHSRLHAIEVVQPALFAMEYALAALWRAWGIHP
                     SVVIGHSMGEVAAACVAGALSLRDAVAVICRRSDLLRAIAGQGGMVQVELPLAETEAA
                     IAGRSDRISVAASNSPQQTVVAGDPDALAELVAELQARGVVARSVKVDIASHSPQVDP
                     LRPALLRALAELSPTTAAIPMMSTVRAERVDGRTLDAAYWADNLRQPVRLHQGVAQLL
                     AEGHTLFLEVSPHPILTAGLQQTLAG"
BASE COUNT          334 a          951 c          863 g          326 t
ORIGIN      
        1 cgcggctgcg gtacgcgacg cccacgagcc gatcgccatc gtcggcgcct cgtgccggct
       61 gcccggcgac gtcgactcgc cgcgcgcgtt ctggtcgctg atggacggcc gcgtcgacgc
      121 gatcaccgag gttccgggcg agcgctggga catcgacgag tggtacgacg ccgacttcca
      181 cgcgcccggc aagatgaaca cccggtacgg cggattcgtg accgggatga ccctgttcga
      241 cgccgagttc ttcggcatca ccccgcgcga ggccagcgtg ctcgacccgc agcagcgcat
      301 gttcctcgag ctgagctggg aggcgctcga gcacgcgggc atcgccccga gctcgcttca
      361 gggcaccgag accggcgtct acgtcgggct gatgtcgcac gactacacgc tgctgcaatg
      421 gggtgacttc acccggttcg acggatcgat cgggatcggg aacaccggca gcgccgccgc
      481 gggccgcgtc tcgtacaccc tcggcaccca cggcccgagc atgagcatcg acaccgcctg
      541 ctcgtcgtcg ctggtcgcca cccacctcgc ttgcaacgcc ctgcgcaatc atgaatgcga
      601 cgtcgccctc gccggcggcg tgtccttgaa cctccacccg gcgatcttcg tcgagttcag
      661 tcgcctgcgc ggcatggcgc ccgacggtcg ctgcaagacc ttctccgccg cggccgacgg
      721 cgtcgcctgg gccgagggct gtggggtgct ggtgctcaag cgcctcagcg acgcgcgccg
      781 cgacggcgac cgcgtgctcg cgctcctgcg cggctccgcc gtcaatcacg acggccgcag
      841 caacggcctg accgcgccga gcgggccggc ccaggaggcc gtggtccgcc gggcgctgcg
      901 cgacgccggg gtcaagccga gcgacatcga ctacgtcgag tcgcacggca ccgggacccc
      961 gatcggcgat ccgatcgagg cgcgagcgct cggcgccgtg ttccgcgggc cgcgggcgtc
     1021 cgggcgcccg ctgtggctcg gcgcggtcaa gaccaacatc ggccacccgc aggccgccgc
     1081 cggcgtcgcc gggatcctca aggtggcgct cgcgctgcgc aaccggcgga tcccggccaa
     1141 cctccacttc gaggcgccga gcccgaacat tcaatgggac gaattctcgc tcgccatccc
     1201 ggtcgcgccg gtcgcctggg aacgcggcga acgcccgcgc atcgccggcg tcagcgcctt
     1261 cggcatcagc gggatcaacg cccacgtggt cgttcaggag gcgccggagg ccgccgacga
     1321 ggacgagccc gcgagcgagt cagtgcacac gcaccacctg ctgccgctgg cggccaagtc
     1381 cccggccgcc ctggccgccc tggtcgcccg gtacctcgcg cacctcgaag cgaacccggg
     1441 tcagggcctg caggacatct gccacaccgc cgcggtcggt cgcgaccatc ccgcgcaccg
     1501 cctcgcgatc gtcagcgagg acctcgcgac cctgcgcgag acgctgctcg ccgtcgtcga
     1561 ccagcaggag cgcgccaacg tcgtcgtcag ccccgagctg cgcaagtcgg gcgccgcgat
     1621 cgtgttcgtg ttccccggcc agggctcgca gtggctcggc atgggccgcg agctgatgcg
     1681 cgagccggcg ttcgccgagg cgatcgcggc ctgcgacgcc gcgatcgccg aggaggcggg
     1741 ctggtcgcta cgcgacgagc tcgccgccga cgaggcgcac agccgactgc acgcgatcga
     1801 ggtcgtccag ccggcgctgt tcgccatgga gtacgcgctg gccgccctgt ggcgcgcgtg
     1861 gggtatccac ccgagcgtcg tgatcgggca cagcatgggc gaggtcgcgg cggcctgcgt
     1921 cgccggggcg ctgtccttgc gcgacgcggt cgccgtcatc tgccgccgca gcgacctcct
     1981 gcgcgcgatc gctggccagg ggggcatggt ccaggtcgag ctcccgctcg ccgagaccga
     2041 ggccgcgatc gccggccggt ccgaccggat cagcgtcgcc gccagcaaca gcccgcagca
     2101 gaccgtggtc gccggcgacc ccgacgcgct cgccgagctg gtggccgagc tgcaagctcg
     2161 cggcgtggtg gcccggtcgg tgaaggtcga catcgcctcg cacagcccgc aggtcgatcc
     2221 gctgcgcccc gccctgctgc gcgcgctggc cgagctgtca ccgaccacgg cggcgatccc
     2281 gatgatgtcg acggtccgcg ccgagcgcgt cgatggccgc acgctcgacg ccgcctactg
     2341 ggccgacaac ctgcgccagc cggtgcgcct ccatcagggc gtcgcccagc tgctcgcgga
     2401 gggacacacg ctgttcctcg aggtgagccc gcacccgatc ctcaccgccg gcctccagca
     2461 gacgctcgcc ggca
//
LOCUS       DASWNJ010001766         3947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_315685, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001766 DASWNJ010000000
VERSION     DASWNJ010001766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315685"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2592)
                     /gene="leuS"
                     /locus_tag="VGB85_29540"
     CDS             complement(<1..2592)
                     /gene="leuS"
                     /locus_tag="VGB85_29540"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEY0138278.1"
                     /translation="MTYDFRAIEARWQSFWDDHHTFRADIDPERPKYYVLDMFPYPSG
                     DGLHVGHPEGYTATDIVARYKRMRGFNVLHPMGWDAFGLPAERYAMKNGVHPAVRTAE
                     CIRNFKRQLKTLGFSYDWEREVDTTDPRYYKWTQWIFLQIWGAWYDAEAGRARPIAEL
                     PIPADITAQGEAAVRKYIDGKRLAYLHDAPVNWCPDLRVVLANEEVAEQVEKGFTVVR
                     RPMRQWMMRITAYAERLISDLEGLDWPASVLDMQRHWIGRSDGAEVRFQIADRDDVIE
                     VFTTRPDTLFGATYMVLAPEHPLVATITTGEQTSAVREYVDKAARRSERDRQAEAAEG
                     QKTGVFTGAHAINPATGQPVPVWISDYVLMGYGTGAIMAVPGHDERDHAFARAMSLPI
                     VEVVSGGASVQDAAHTEAGTAVNSGFLDGLATAAAKEKMISWLEAQGLGRRRVTYKLR
                     DWLFSRQRYWGEPFPLLHQQDGAVVPVPADQLPVTLPELDDFQPSETGEPPLSKATQW
                     VHLPDGSLRETNTMPQWAGSCWYYLRFMDPANDAAPFSKAAADYWLPVDLYIGGAEHA
                     VLHLLYARFWHKVLFDLGHVGTREPFARLVNQGMVLGATYLPKDRRRGPDGEPQVWGP
                     ADVEQVTGTDEWRVKATGETVDVQWDKMSKSRGNVVNPDDVVQQFGADTMRLYEMFMG
                     PLEHSAPWQTEGVAGCHRFLQRAYRLAFETADDGTPDRLRALAPGEGSDRQRRLLHRT
                     IQAVTERIDRLAFNTAISAMMVFVRDIVDEGETLHHDTLAQFCLLLAPFAPHLAEEIW
                     RAIGKTTSLAHELWPAADDAMLVDATFPLVVQIQGKRRAELAIPQGTSEDDIKARVLA
                     DAEVVRHL"
     gene            complement(2785..3297)
                     /locus_tag="VGB85_29545"
     CDS             complement(2785..3297)
                     /locus_tag="VGB85_29545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138279.1"
                     /translation="MRPPSLGLLFLLPACLLLACGGVEPDDSAGSTGSSDTGDTAGSE
                     PHATFTGKVDAIGLCGTEGATVVTFLARRVGCESLTAPCTIKIDPYASWSGDAVTCPS
                     SQTALDMHVEVPMSGRYQIEALTMTPSGFQSLCYGENADVPTVVTAAQLDARAEIFVE
                     QTAKPCPDPA"
     gene            complement(3313..>3947)
                     /locus_tag="VGB85_29550"
     CDS             complement(3313..>3947)
                     /locus_tag="VGB85_29550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138280.1"
                     /translation="DATRVRALGLRLAQDLQRVTLAQAAVCTAMATGKDKGPVAQNAP
                     AGEVLRQSTRHAQTLFGALTKLWSTLGPDALANAGLGEVDAAIASVGAAQPKVQAACK
                     GRYHGSGSLATEAATTTKLDLDKLYRAPILGAFEDVLAKGKTWSTVEKEVGDRLSALP
                     TARRKVWQDILKSSKAAVEASATKTTGKGATGDKHVEKQGKPTGGKGKAK"
BASE COUNT          530 a         1360 c         1420 g          637 t
ORIGIN      
        1 gaggtggcgg acgacctccg cgtcggcgag cacgcgcgcc ttgatgtcgt cctcgctggt
       61 gccctgcggg atcgccagct cggcgcgccg cttgccctgg atctggacca cgagcgggaa
      121 ggtggcgtcg acgagcatcg cgtcgtcggc cgcgggccac agctcatgcg cgagcgaggt
      181 cgtcttgccg atcgcgcgcc agatctcctc cgcgaggtgc ggggcgaagg gcgcgagcag
      241 caggcaaaac tgcgccagcg tgtcatggtg cagggtctcg ccctcgtcga cgatgtcgcg
      301 gacgaacacc atcatggcgc tgatcgccgt gttgaacgcg agccggtcga tgcgctcggt
      361 caccgcctgg atcgttcggt gcaacagccg gcgctggcgg tcgctgccct cccccggcgc
      421 cagcgcgcgc aggcgatccg gggtgccgtc gtcggctgtc tcgaaggcca ggcgataggc
      481 gcgctgcagg aagcggtggc agccggcgac gccctcggtc tgccacggcg ccgagtgctc
      541 gagcggaccc atgaacatct cgtacagacg catggtgtcg gcgccgaact gctggacgac
      601 gtcgtcgggg ttgacgacgt tgccgcggct cttggacatc ttgtcccact gcacgtcgac
      661 ggtctcgccg gtcgccttga cccgccactc gtcggtgccc gtcacctgct cgacgtccgc
      721 cggaccccac acctgcggct cgccgtcggg cccgcggcgg cggtccttgg gcagataggt
      781 cgcgccgagc accatgccct ggttgacgag gcgcgcgaac ggctcgcggg tgccgacgtg
      841 gccgaggtcg aacaggacct tgtgccaaaa gcgggcgtac agcaggtgca gcacggcgtg
      901 ctcggccccg ccgatgtaca ggtcgaccgg cagccagtag tcggcggcgg ccttcgagaa
      961 cggcgcggcg tcgttggccg ggtccatgaa gcgcaggtag taccagcagc tgccggccca
     1021 ctgcggcatc gtgttggtct cgcgcaggct gccgtcgggc aggtggaccc actgcgtcgc
     1081 cttgctcagc ggcggctcgc cggtctcgct cggctggaag tcgtcgagct cgggcagcgt
     1141 gacgggcagc tggtccgcgg ggacgggcac gacggcgccg tcttgctgat gcagcagcgg
     1201 gaacggctcg ccccagtagc gctggcgcga gaacagccag tcgcgcagct tgtaggtgac
     1261 gcgccggcgc ccgaggccct gcgcctcgag ccaggagatc atcttctcct tggccgccgc
     1321 ggtcgcgagg ccgtcgagga agcccgagtt gaccgcggtg ccggcctcgg tgtgcgcggc
     1381 gtcctggacc gacgcgccgc cggacacgac ctcgacgatc ggcagagaca tcgcccgcgc
     1441 gaacgcgtgg tcgcgctcgt cgtgacccgg caccgccatg atcgcgccgg tgccatagcc
     1501 catcagcacg tagtcggaga tccacaccgg gaccggctgt ccggtggccg ggttgatcgc
     1561 gtgcgcgccg gtgaagacgc ccgtcttttg cccctcggcc gcctcggcct ggcggtcgcg
     1621 ctcgctgcga cgcgccgcct tgtcgacgta ctcgcggacc gccgaggtct gttccccggt
     1681 ggtgatcgtg gcgacgagcg ggtgctccgg cgcgagcacc atgtaggtcg cgccgaacag
     1741 cgtgtcgggg cgcgtggtga acacctcgat gacatcgtcg cggtccgcga tctgaaagcg
     1801 cacctcggcg ccgtcgctgc ggccgatcca gtggcgctgc atgtcgagca cgctcgccgg
     1861 ccagtcgagc ccctcgaggt cacttatcaa acgctcggcg taggcggtga tccgcatcat
     1921 ccactggcgc atcggccggc gcacgacggt gaagcccttc tcgacctgct cggcgacctc
     1981 ctcgttggcg aggaccactc gcaggtccgg acaccagttg accggcgcgt cgtgcaggta
     2041 ggcgagccgc ttgccgtcga tgtacttgcg gacggcggcc tcaccctggg cggtgatgtc
     2101 ggccgggatc gggagctcgg cgatcggacg cgcacgcccg gcctcggcgt cgtaccaggc
     2161 gccccagatc tgcaggaaga tccactgcgt ccacttgtag taacgcgggt cggtggtgtc
     2221 gacctcgcgc tcccagtcgt acgagaaacc cagcgtcttg agctgacgct tgaagttgcg
     2281 gatacactcg gcggtccgca ccgccgggtg cacgccgttc ttcatcgcgt aacgctcggc
     2341 cggcagaccg aaggcgtccc accccatcgg gtgcaacacg ttgaacccac gcatccgctt
     2401 gtaccgcgcg acgatgtcgg tcgcggtgta accctccgga tgaccgacgt gcagaccgtc
     2461 cccggacggg tacgggaaca tgtcgaggac gtaatacttc ggacgctccg gatcgatgtc
     2521 ggcccggaaa gtgtggtggt cgtcccagaa gctctgccag cgggcttcga tggcgcgaaa
     2581 atcgtaggtc atgggggggc gtacgctaat ctttcgcgcg gaaaaagcca aggggagcgc
     2641 ggcctggctg acttcgctgc gccgtcgagg ctgcttgcgc ggcccggtgc gcccggggtg
     2701 cctgtcctgg cggacatgtc cctgagtgcc aggggccatg cgcctgcgcg cggcgctgct
     2761 cgccaccagc cttgcggacg gtgatcacgc gggatcgggg cagggcttcg cggtctgctc
     2821 gacgaagatc tcggcgcgcg cgtcgagctg cgctgcggtg accaccgtcg gcacgtcggc
     2881 gttctcgccg tagcacaggc tctggaagcc cgacggggtc atcgtcagcg cctcgatctg
     2941 gtagcggccc gacatcggga cctcgacgtg catgtcgagc gcggtctggc tgctgggaca
     3001 ggtgacggcg tcgccgctcc acgaggcgta cgggtcgatc ttgatcgtgc acggcgccgt
     3061 gagcgactcg cagccgacgc ggcgcgcgag gaaggtgacg acggtcgcgc cctcggtgcc
     3121 gcacaggccg atcgcgtcga ccttgccggt gaaggtcgcg tgcggctcgc tccctgcggt
     3181 gtcgccggtg tcactgctcc cggtgctccc ggcgctgtcg tcgggctcga cgccgccgca
     3241 cgcgagcagc aggcaagccg ggaggagaaa caagagaccg aggctcgggg ggcgcatgga
     3301 cggaagcttg acctacttcg ccttgccctt gccacccgtc ggcttgccct gcttctcgac
     3361 gtgcttgtcg ccggtcgcgc ccttgccggt cgtcttggtg gcgctggcct cgacggcggc
     3421 cttgctcgac ttgaggatgt cctgccacac cttgcggcgc gcggtcggca gcgcgctcag
     3481 gcggtcgccg acctccttct cgacggtcga ccaggtcttg cccttggcga gcacgtcctc
     3541 gaaggcgccg aggatcggcg cgcggtacag cttgtcgagg tcgagcttgg tggtcgtcgc
     3601 cgcctcggtc gccagagatc cactgccgtg gtaacgaccc ttgcacgcgg cctgcacctt
     3661 gggctgcgcg gccccgacgc tggcgatcgc agcgtcgacc tcgccgaggc cggcgttggc
     3721 cagcgcgtcc gggccgagcg tcgaccacag cttcgtcagc gcgccgaaca gcgtctgcgc
     3781 gtggcgggtg ctctggcgca ggacctcgcc ggcgggcgcg ttctgggcca ccgggccctt
     3841 gtccttgccg gtcgccatcg ccgtgcacac ggccgcctgc gcgagcgtga cgcgctgcag
     3901 gtcctgcgcg agacggagcc cgagcgcgcg gaccctcgtg gcgtccg
//
LOCUS       DASWNJ010001767         4026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_667948, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001767 DASWNJ010000000
VERSION     DASWNJ010001767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667948"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..2314
                     /locus_tag="VGB85_29555"
     CDS             287..2314
                     /locus_tag="VGB85_29555"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY0138281.1"
                     /translation="MKASISKCCGWLIVAALCGAPASASAQTAAPAAGAPAAPATKAA
                     AFVYRVGMDDPERHEFSLELNFSDIPGTSVDLQLPKWNPGAYRLTEAHRNVRGMVAET
                     PGGKPVPVVKVDEITWRVSHGGQPFRVRYRVYRGSYNGISGAYLDDEFGFFNGVYLFL
                     YAVGHKDRPIELRVDDLPVAGAQVVTGLPRVGQGKSFRAANYDVLVDAPVHAGKVDLI
                     PFAVGKTKFRLAMAGIGNYSEKKLIADFTKLAQTTFAVFGDGPEAVPFDDYTFIFHLR
                     PGNRAGLEHLNSTVIGADPFVFGDPETYRGLLRLAAHELFHAWNVKRIRPTVLGPFAY
                     EREVHTSMLWFSEGFTSYYAWVLMARAGLSGEPETLEYLAENIKKLQESPGRKTMTVE
                     QSSWETWLKPEDAGNSYVDYYTKGMLIGAMIDLEIRRISGGQRSVDTVMRELWARHLR
                     VGTGIGPAELEQIFVTQGGAAGAEVSDLFRRYVHGFDEIDYNRHLGLAGYRLEVSQSE
                     AAGDIEATIGERVGDGAAVIELVRAGGPAEKAGLSNNDVLVAIDGLKVTAKDAPKQIK
                     AMAGNSKHTFTLLRGPRLLTKTVVPVTSGQTKYKVVSLPSVSYEQKLMRQGWLGYTAQ
                     TATGSQDQLREAAMRLFTSDPTVGAEEAVPTTAPPRAKKAKKPKASKPPAK"
     gene            complement(2315..>4026)
                     /locus_tag="VGB85_29560"
     CDS             complement(2315..>4026)
                     /locus_tag="VGB85_29560"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY0138282.1"
                     /translation="IACRLIKVPAIAALLAAGVVVGPVGLALVESSVNIDTIADLGLV
                     LLLFVIGLEVNLASLLRSGRALLVSGLLQVPLTVAIAWLLFAGLRQTGWDMLQGDYTA
                     LYLGLTCAFSSTLLVAGHLQSQRQMDTRSGRLAVGLLIFQDIWAVVVLALQPNFAHLD
                     PTPVLLTLGGVAVMLAVAAVFTRHVLPRAFRAVGDAPQLVLTLAAAWCFGLGLFGLHL
                     GDLLRLIGLDLDISISLGMGALIAGTSIATFPHAYEVLTKVGSLRDFFVTLFFVALGM
                     SIPLPHGFDVVALALLLGVIAIALRQVLFLPLLLRAGVDVRRAAETSIKLSQISEFCL
                     VIMYLGMDLGHVDERFTSAVIFAFVLTAVLSPALFRLAERSFPRLVRTVARVRGHAPS
                     VPQDAPPVRPRLAILGFDRVGAALFCELERTSPDLLECTVVVDLDVRIREAIEARGAR
                     ILNADLANLDALRHAGIDQAELIVIALTDDQLKGTDNHHITRSLRADNPDATIIASAT
                     RGAQVAGLIEAGASHVYMWPAETAAGLVPAIEAALGGELDEFTRRSERRHGPLAGRCG
                     LLD"
BASE COUNT          672 a         1225 c         1627 g          502 t
ORIGIN      
        1 caggacggcg atctcgccgg tcgaccacag gccgtgggtg cgaaagccgg ggatcagggc
       61 gaggccggcg aggccagcgg cgaggccggc gaggcgccag gacccggagg tcggggaccc
      121 ggcggtcggg gactcggagg cggcgagcgc tgggtcgttt ggggccacgg ggcgcgcgag
      181 agtagcagga gcggtgggtc acgggcggcc tgctgggcca gcggagcacg gcgcgcggcg
      241 cgaggacttg cacggccacc cgggcacgag gctatgctgg gcggcgatga aggcatcgat
      301 ctcgaagtgc tgtgggtggc tgatcgtggc ggcgctgtgc ggggcgccgg cgtcggcgtc
      361 ggcccagacg gcagcgccgg ccgcgggcgc acccgcggcc ccggcgacga aggcggcggc
      421 cttcgtgtac cgggtcggca tggacgaccc ggagcggcac gagttctcgc tcgagctcaa
      481 cttcagcgac atcccgggca ccagcgtcga cttgcagctg ccgaagtgga acccgggcgc
      541 gtaccggttg acggaggcgc accgcaacgt gcgcgggatg gtggccgaga cgccgggtgg
      601 caagccggtg ccggtggtca aggtcgacga gatcacgtgg cgggtcagcc acggcgggca
      661 gccgtttcgc gtccgttatc gggtgtatcg cggcagctac aacgggatct ccggcgcgta
      721 cctcgacgac gagttcggct tcttcaacgg ggtgtacctg ttcctgtacg cggtcggtca
      781 caaggatcgg ccgatcgagc tgcgcgtcga cgacctgccg gtcgccgggg cgcaggtcgt
      841 caccgggctg ccgcgcgtgg gccagggcaa gagctttcgc gcggccaact acgacgtgct
      901 cgtcgacgcg ccggtgcacg cgggcaaggt cgacctgatc ccgttcgcgg tcggcaagac
      961 caagtttcgc ctggcgatgg cggggatcgg gaactacagc gaaaagaagc tgatcgccga
     1021 cttcacgaag ctggcccaga cgaccttcgc ggtgttcggc gacgggccgg aggcggtgcc
     1081 cttcgacgac tacaccttca tcttccacct gcgtccgggc aaccgcgcgg ggctcgagca
     1141 cctcaactcg acggtgatcg gcgccgaccc gttcgtgttc ggcgaccccg agacgtaccg
     1201 cgggctgctg cggctcgccg cgcacgagct gttccacgcg tggaacgtca agcggatccg
     1261 gccgacggtg ctcgggccgt tcgcgtacga gcgcgaggtc cacacctcga tgctgtggtt
     1321 ctcggagggc ttcaccagct actacgcgtg ggtgctgatg gcgcgcgcgg ggctcagcgg
     1381 ggagccggag acgctcgagt acctggcgga gaacatcaag aaattgcagg agtcgccggg
     1441 tcgcaagacg atgacggtcg agcagtcgag ctgggagacc tggctcaagc ccgaggacgc
     1501 gggcaacagc tatgtcgatt actacacgaa ggggatgttg atcggcgcga tgatcgacct
     1561 cgagatccgg cggatctcgg gcgggcagcg cagcgtcgac acggtgatgc gcgagctgtg
     1621 ggcgcggcac ctgcgggtcg gcacgggcat cggtccggcc gagctcgagc agatcttcgt
     1681 cacccagggc ggggcggccg gggccgaggt ctcggacctg tttcgccgct atgtgcacgg
     1741 cttcgacgag atcgactaca accgccacct cgggctcgcg ggctatcgcc tcgaggtgtc
     1801 gcagagcgag gcggccggcg acatcgaggc gacgatcggc gagcgcgtcg gcgacggggc
     1861 ggcggtgatc gagctggtgc gcgccggcgg cccggccgaa aaggcggggc tgagcaacaa
     1921 cgacgtgctg gtggcgatcg acgggctcaa ggtgaccgcc aaggacgcac cgaagcagat
     1981 caaggcgatg gccggcaaca gcaagcacac gttcacgctg ctgcgagggc cgcggctgct
     2041 gaccaagacg gtggtgccgg tgacgagcgg acagaccaaa tacaaggtcg tgtcgctgcc
     2101 ctcggtcagc tacgagcaga agctgatgcg gcagggctgg ctcggctaca ccgcccagac
     2161 ggcgaccggc tcgcaggacc agctgcgcga ggcggcgatg cgcctgttca cgagcgaccc
     2221 gacggtcggg gcggaggagg cggtgccgac gacggcgccg cctcgggcga agaaggcgaa
     2281 gaagccgaag gcgagcaagc ccccggcgaa gtagtcagtc gagcaggccg cagcgcccgg
     2341 cgagcgggcc gtggcggcgt tcgctgcggc gggtgaactc gtcgagctcg ccgccgaggg
     2401 cggcttcgat cgcgggtacg agccccgcgg cggtctcggc gggccacatg tacacgtggc
     2461 tggcgccggc ctcgatcagg ccggcgacct gggccccgcg agtggcggag gcgatgatcg
     2521 tcgcgtcggg gttgtcggcg cgcagcgagc gggtgatgtg gtggttgtcg gtgcccttga
     2581 gctggtcgtc ggtcagggcg atgacgatca gctcggcctg gtcgatgccg gcgtggcgca
     2641 gggcgtcgag gttggcgagg tcggcgttga ggatgcgagc gccgcgggcc tcgatcgcct
     2701 cgcggatgcg gacgtcgagg tcgacgacga cggtgcactc gagcaggtcg gggctggtgc
     2761 gctcgagctc gcagaacagg gcggcgccga cgcggtcgaa gccgaggatc gccaggcgcg
     2821 ggcgcacggg cggggcgtcc tgcgggaccg agggcgcgtg gccgcggacc cgggcgacgg
     2881 tgcggaccag ccgggggaag ctgcgctcgg cgaggcggaa cagggccggg ctgagcacgg
     2941 cggtcagcac gaaggcgaag atgacggcgc tggtgaagcg ctcgtcgacg tgcccgaggt
     3001 ccatgccgag gtacatgatg acgaggcaaa attcggagat ctgcgacagc ttgatcgagg
     3061 tctcggcggc gcggcggacg tcgacgccgg cgcgcagcag cagcggcagg aacaggacct
     3121 ggcgcagcgc gatggcgatg acgccgagca gcagcgcgag cgcgacgacg tcgaagccgt
     3181 gcggcagcgg gatgctcatg ccgagggcga cgaagaacag ggtgacgaag aagtcgcgca
     3241 ggctgccgac cttggtcagc acctcgtagg cgtgcgggaa ggtggcgatc gaggtgccgg
     3301 cgatcagggc gcccatgccg agcgagatcg agatgtcgag gtcgaggccg atcagtcgca
     3361 ggaggtcgcc gaggtgcagg ccgaacagac cgaggccgaa gcaccaggcg gcggcgaggg
     3421 tcagcaccaa ctgcggggcg tcgccgacgg cgcgaaaggc ccgcggcagg acgtggcggg
     3481 tgaacacggc ggcgacggcg agcatcacgg cgacgccgcc gagggtcagc agcaccggcg
     3541 tcggatcgag gtgggcgaag ttcggctgca aggcgagcac gacgacggcc cagatgtcct
     3601 gaaagatcag caggccgacg gcgaggcggc ccgagcgggt gtccatctgg cgctgcgatt
     3661 gcaagtggcc ggcgaccagc agggtcgagg agaaggcgca ggtgagtccg aggtagagcg
     3721 cggtgtagtc gccctgcagc atgtcccagc cggtctggcg caggccggcg aacagcagcc
     3781 acgcgatcgc gacggtcagc gggacctgca gcaggccgct gacgagcagg gcgcggccgc
     3841 tgcggagcag gctggcgagg ttgacctcga ggccgatgac gaacagcagc agcacgaggc
     3901 cgaggtcggc gatcgtgtcg atgttgacgg agctctcgac gagcgcgagg ccgacggggc
     3961 cgacgacgac gcccgcggcg agcagggcgg cgatcgccgg gaccttgatc aggcggcagg
     4021 cgatcg
//
LOCUS       DASWNJ010001768         1506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_171157, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001768 DASWNJ010000000
VERSION     DASWNJ010001768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171157"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..542
                     /locus_tag="VGB85_29565"
     CDS             222..542
                     /locus_tag="VGB85_29565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138283.1"
                     /translation="MKKLLFLVAFVGSVATGAACNNSLTESCKDFRSARDACEDQNGD
                     PAPMYGFDLCDNIDPDCEEFYNCAAAAPCEQNKNDDKWRLQLAAAECKQPENKECTDA
                     DLRM"
     gene            complement(697..>1506)
                     /locus_tag="VGB85_29570"
     CDS             complement(697..>1506)
                     /locus_tag="VGB85_29570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138284.1"
                     /translation="RVRAAAREAFLAYVSGPPPRAERKTVRLLGGHTTEAPARLTYRD
                     FARLAIRERVASEQPDLLEPECRPITPEGLRDHACEAQPERLATAYFERVELARRARE
                     QAQVDEALAQPDRAATVAMLDALLADNPELGMRAQLVPSYEAAADEAEAAGDLRRASG
                     LLRKSAALLAEADPVRADQLRLRALLVEAQIAELPAQGRAMLLATAESLAPDDPAVHA
                     ALAQIAERPVTVEDAALRDRLTILLLAVFGALATLSLFGWLLRRTQPAAVA"
BASE COUNT          240 a          510 c          565 g          191 t
ORIGIN      
        1 gccgcgagta cttcgcgtgc gccgcgaagg cggagtgcaa ggagtcgggc ggccgctaca
       61 ccctcgactc caaggcctgc accatgcccg agggcaagca gtgccttccg ggctcgtgaa
      121 agatccggac ggcgatcgga cggggcgctg gtagggctgg ccaaggcgcg ggcaaaagtg
      181 ctaccaagaa ggcgcccgtc ccccgagaaa gcgagaagcc cgtgaagaag ctgctgttcc
      241 ttgtcgcctt cgttgggtcg gtcgccaccg gcgccgcctg caacaactcg ctcaccgagt
      301 cctgtaagga cttcaggtcg gcacgcgacg cctgcgagga tcaaaacggc gatcccgcgc
      361 cgatgtacgg cttcgacctg tgcgacaaca tcgaccccga ctgcgaggag ttctacaact
      421 gcgccgcggc ggccccgtgc gagcagaaca agaacgacga caagtggcgc ctccagctcg
      481 ccgccgccga gtgcaagcag cccgagaaca aagagtgcac cgacgccgac ctgcgcatgt
      541 gagcgcggcg cagtgaaagc atgagaaagc ttgagaaagc atggtcgagg cgaccggacg
      601 cgtgacgcga ccgtcgcctc gattgtcatt taacaagggt tgagcgatcg aggcggccgg
      661 acgcgtgacg cgaccgtcgc ctcgtcgctt ttgggttcag gcgacagcag cgggctgcgt
      721 gcggcgtagc agccagccga acagcgacag cgtggcgagg gcgccgaaca cggcgagcag
      781 caggatcgtc aggcgatcgc gcagcgcggc gtcctcgacg gtcacgggtc gctcggcgat
      841 ctgggccagc gcggcgtgca cggcggggtc gtcgggcgcg aggctctcgg cggtcgccag
      901 caacatcgcc ctgccctgcg ccggcagctc ggcgatctgg gcctcgacga gcagcgcgcg
      961 caggcgcaat tgatcggcgc gaaccgggtc ggcctcggcg agcagcgcgg ccgacttgcg
     1021 cagcaggccc gaggcgcgac gcaggtcgcc ggcggcctcg gcctcgtcgg cggccgcctc
     1081 gtagctgggc accagctgcg cgcgcatgcc gagctcgggg ttgtcggcga gcagggcgtc
     1141 gagcatcgcc acggtcgcgg cgcggtcggg ctgggccagc gcctcgtcga cctgggcctg
     1201 ctcgcgcgcc cgccgggcga gctcgacacg ctcgaagtag gcggtggcga gccgctcggg
     1261 ctgcgcctcg caggcgtggt cgcgcaggcc ctcgggcgtg atcggccggc actcgggctc
     1321 gagcagatcc ggctgctcgc tggcgacgcg ctcgcggatc gccaggcgcg cgaagtcgcg
     1381 gtaggtcagg cgcgctggcg cctcggtcgt gtgcccgccg agcaggcgca cggtcttgcg
     1441 ctcggcccgg ggcggcggac cgctgacgta ggcgaggaac gcctcacggg ccgccgcacg
     1501 cacgcg
//
LOCUS       DASWNJ010001769         5839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_701815, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001769 DASWNJ010000000
VERSION     DASWNJ010001769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5839
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701815"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..2004
                     /locus_tag="VGB85_29575"
     CDS             148..2004
                     /locus_tag="VGB85_29575"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HEY0138285.1"
                     /translation="MSRGSRSLLNLLLFAGLSHACATAAPTTKPAPAAHDAAASFTWH
                     AWTPASFATAQRERRILLVNVAASWCHWCHVMDHETYADAEVAALLRDHFMPIRVDSD
                     ARPDLAERYSDWGWPATAVLTSDARPVLELRGYQDPREFAALLRGLVADRDAGKLQGR
                     RPAPPAPVRDADLVALRQAAYKQLDGLYHEEIGGWGRKQKYPLAALDELALLRAQVFG
                     EKQWHERALTTLGNEVKLIDPVWGGMYQYSVGGTWDDPHFEKIGAIQAGALEALALAY
                     RRTGDARWRAAAHLQRSYLLDRMQHPQGGFYTSQDADLQRPGQPPVLGADYYALDDAG
                     RRKLGVPRVDENRYADLNGRTIVALCRLYAAVPGPDGKDDVAALAAAQTAAEHLLRAH
                     RAASGGFKHAAEDTGLLYLRDQAAVGRALLALYRVTGDARWLDEARSLAKFMLEALAD
                     RERGGFFAHTLDPNAVGVFAERRKPLEENALAARFMLELHRQVDHLPDPGPHGDAAIA
                     TLRAVSDPSRVREEGRMIGQYALALAEASFTPVDVTVVGKQGDPTVVALHRAALALDD
                     PRTLLNISPPGARYPDIGAPAVYLCTDNSCSSPIKDVAKLPAAAAKFLATVP"
     gene            2200..2568
                     /locus_tag="VGB85_29580"
     CDS             2200..2568
                     /locus_tag="VGB85_29580"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEY0138286.1"
                     /translation="MQVPVQITFHQLEASPEVETRIQEKVAKLEQFYNGIISCRVVVE
                     PAAHQHGTGQTIFSVRFELGLPNNKTIVGGGPGTKNDAFDNVYTAITAAFEATKRQLH
                     DHAEKERQFDAPPLDTIPPG"
     gene            complement(2663..4012)
                     /locus_tag="VGB85_29585"
     CDS             complement(2663..4012)
                     /locus_tag="VGB85_29585"
                     /inference="COORDINATES: protein motif:HMM:NF024562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 family protein"
                     /protein_id="HEY0138287.1"
                     /translation="MGALQGSATARAGEADATYRWRAPIEVLQAAPFVYLELPAGAYA
                     HTLQPDLRDLKIVDALGEPVPFALRMPRERQVTADLERSATLYPLPTRPVRGGEWPSP
                     IEVTVAGEQISVKRLAGAPPDPAGSPGWLIDLGEHAPGSPPHTLRLAWSGPPDFTASF
                     EFETSDDLRTWLQHGRGSVMALASTSGSLRQPSLPLPRSAGRFLRLVWTEAGAPPQLT
                     GATAISTAPRDEMPTIVGVEAPDQLVLSPTPADANMLDESSRRALIFDLGGALPVRQL
                     DLRFTRGTVVAPVRLEARARPDEPWHSLGEQVFHRIEQGTDVRTSSPLALQTTLGQVR
                     VVPDPRTPALDPANTKLVVQARLATAIFATRGEPPFTLYAGSSTAAPGALPEVALIPE
                     GEEDRVLFGVATLGPWIEVDDATRHCAQLRPWILWAVLLAGVGGLAWMVRRLIRGSR"
     gene            complement(4035..>5839)
                     /locus_tag="VGB85_29590"
     CDS             complement(4035..>5839)
                     /locus_tag="VGB85_29590"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEY0138288.1"
                     /translation="FLVGSAWGVLKYQPQHFASSEAFLITYFLLFIAIMLMPARHLDG
                     KHEHPTLGVVWLNGSLLFGLPTITFALQYGLVEGDDFGVALSALALAAFYVAMAAYMR
                     PRPRWAATFEAVLTIGTVFLTLVVPFALDARSTAGAWALEAAGLVWLGFRQQRVVSRV
                     CGYVLLLLACGPLLHAFDRLPTPVEIANGHLFASVLLAVGALASAYFVQRFTAATKSN
                     ERVLEPFLIAWGTLWLLVAAGVEIDAFVLESRHWLAAWLTTVSAIALSYVALADALRW
                     KNITAPVLAHAPVLALCVAVSAEAFTSPGAWGGWWAWPLALLTHVVVLWRAAPRWSTA
                     GQTVAHATGALVLAALGSLQGRAITRAWGDVDSAWAWLGWLAVPALLLLLLTRPAALR
                     VWPVRAAPVAYRAIAGGVLTAGLFLWILAANFVSNGSAAPLTYVPLLSPLDLGVGVAL
                     LAAWLWIRGDAVRDTLGDSVDALRWMFWAVGFLWLNAAMVRGFHHHGGVPFDADTMAA
                     SQAVQTGFTLLWAVLALIVMWIAVRRAARAPWMVGAGLLALVIVKLLAVDLAASDTVT
                     RIISFIGVGVLMLVIGFVAPLPATGRPHAEKPEK"
BASE COUNT          905 a         2136 c         2074 g          724 t
ORIGIN      
        1 cgcctccggg tgagagcgtc ccggtcgcgc cgccgcccgg ctgagcctgt cccgaaggac
       61 aggcccccgg tgcgcccgcg cgcggcccat gtcacgaccc gcggggtcgc gtcgatctac
      121 gcggccggtc ccgtcgtaaa atgccgcatg tctcgcggct cgcgctcgct cctgaacctg
      181 ctgctgttcg ccggtctgtc gcacgcctgc gccaccgcgg cgccgacgac caagccggcg
      241 cccgcggccc acgacgccgc ggcgagcttc acctggcacg cgtggacccc ggcgagcttc
      301 gccacggccc agcgcgagcg gaggatcctg ctcgtcaatg tcgcggcgag ctggtgtcac
      361 tggtgtcacg tgatggacca tgagacctac gccgacgccg aggtcgccgc gctgctacgc
      421 gaccacttca tgccgatccg cgtcgactcc gacgcgcgcc ccgacctcgc cgagcgctac
      481 tccgactggg gctggccggc gaccgccgtg ctgaccagcg acgcgcgccc ggtgctcgag
      541 ctgcgcggct atcaggaccc gcgggagttc gcggcgctgc tgcgcggcct ggtcgccgac
      601 cgcgacgccg gcaagctcca gggccgccgc ccggccccgc cagcgccggt ccgcgacgcc
      661 gacctggtgg cgctgcgaca ggccgcatac aagcagctcg acggcctcta tcacgaggag
      721 atcggcggct ggggtcgcaa gcagaagtac ccgctggcgg ccctcgacga gctggccctg
      781 ctgcgcgcgc aggtgttcgg cgagaagcag tggcacgagc gggccctgac gaccctgggc
      841 aacgaggtca agctgatcga cccggtgtgg ggcggcatgt atcagtactc ggtcggcggc
      901 acctgggacg acccgcactt cgagaagatc ggcgcgatcc aggccggcgc gctcgaggcc
      961 ctcgcgctcg cctatcgccg caccggcgac gcccgctggc gggccgccgc gcacctgcag
     1021 cgcagttatc tgctcgaccg catgcagcac ccgcagggcg gcttctatac cagccaggac
     1081 gccgacctcc agcgcccggg ccagccgccg gtgctcggcg ccgactacta cgcgctcgac
     1141 gacgccggcc ggcgcaagct cggggtcccg cgcgtcgacg agaaccgcta cgccgacctc
     1201 aacggccgca cgatcgtcgc gctgtgccgg ctgtacgcgg ccgtcccggg acccgacggc
     1261 aaggacgatg tcgccgccct cgccgccgcg caaacggccg ccgagcacct gctgcgcgcg
     1321 caccgggccg ccagcggcgg cttcaagcac gcggccgagg acaccggcct gttgtatctg
     1381 cgcgaccagg cggcggtcgg ccgggccctg ctggcgctgt accgcgtcac tggcgacgcg
     1441 cgctggctcg acgaggcgag gtccctggcg aagttcatgc tcgaggcgct cgccgaccgc
     1501 gagcgtggag gcttcttcgc gcacacgctc gacccgaacg ccgtcggggt gttcgcggag
     1561 cgccgcaagc cgctcgagga gaacgccctc gccgcgcgct tcatgctcga gctgcaccgt
     1621 caggtcgacc acctgcccga tcccggcccc cacggcgacg ccgcgatcgc cacgctgcgc
     1681 gccgtctcgg atccctcacg cgtgcgcgag gagggccgca tgatcgggca gtacgcgctc
     1741 gcgctcgccg aggccagctt cacaccggtc gatgtcaccg tcgtcggcaa gcagggcgac
     1801 ccgaccgtgg tcgccctgca ccgcgccgcg ctcgcgctcg atgatcctcg cacgctcctc
     1861 aacatcagcc cgccgggcgc ccgctacccc gacatcggcg cgcccgccgt gtacctgtgc
     1921 accgataact cgtgctcgtc gccgatcaaa gacgtcgcca agctgccggc cgcggcggca
     1981 aaattcctgg cgaccgtgcc ctgatgaacc ccgtgtcgat ccgggggatc tggcgttttg
     2041 tgtggtagcg caggctccgc agtgcggcgg cggccccggc aaatgctccc gccgaagggc
     2101 gcagaaggcg gccctgtgcg gccctgaacg caataggtca cggctgtcac gtgacgcccc
     2161 gggcagacgt ggcacggaaa ctatcagcca aggagcgcca tgcaagtacc cgtacagatc
     2221 accttccacc agctggaagc ctctcccgaa gtcgagacac ggatccagga gaaggtcgcc
     2281 aagctcgagc agttttataa cggcatcatc agctgccgcg tggtcgtcga gcctgcggcg
     2341 caccagcacg gcaccggcca gacgatcttc tcggtccgct tcgagctcgg cttgcccaac
     2401 aacaagacga tcgtcggcgg tggacccggc accaagaacg acgccttcga caacgtgtac
     2461 accgcgatca cggcggcctt cgaggcgacc aagcgccagc tgcatgacca cgcggagaag
     2521 gagcgccagt tcgacgctcc gccgctcgac accatcccgc cgggctgatc cctcgccctt
     2581 gggcccgctc tcgtgttcgc atggggcggg cttcgatcgc gctcgcgttc ggtgacgcgg
     2641 ggccgcacgc cggggccgag ctctaccgag atccacggat gagtcgccgc accatccagg
     2701 cgaggccgcc gaccccggcg agcagcacgg cccagaggat ccacgggcgg agctgggcgc
     2761 agtggcgggt ggcgtcgtcg acttcgatcc atgggccgag agtggcgacg ccgaacagga
     2821 cgcggtcttc ctcgccctcg gggatcaacg cgacctccgg cagggcgccg ggggctgcgg
     2881 tcgacgagcc ggcgtacagc gtgaatggtg gctcgccacg cgtcgcgaag atcgccgtgg
     2941 cgagccgcgc ctggaccacc agcttcgtgt ttgcgggatc gagggcaggc gtgcgtgggt
     3001 ccgggaccac gcggacctgg ccgagggtgg tctgcagggc gagcggtgat gaagtgcgga
     3061 cgtcggtgcc ctgctcgatg cggtggaaca cctgctcacc gagcgagtgc cacggctcgt
     3121 cgggccgcgc gcgggcctcg agccgcacgg gggcgacgac ggtgcctctt gtaaaccgca
     3181 ggtcgagctg gcgcaccggc aacgcgccgc cgaggtcgaa gatcagggcg cgccgcgagc
     3241 tctcgtcgag catgttcgcg tcggcgggtg tcggggacag cacgagctgg tccggggcct
     3301 cgacgccgac gatggtgggc atctcgtcgc ggggcgccgt cgagatcgcc gtcgcgccag
     3361 tcagctgcgg cggtgcgccg gcttcggtcc agaccaggcg caggaacctc ccggcgctgc
     3421 gcggcagcgg caggctcggc tgtcgcagcg agcccgacgt cgaggccagg gccatcacgg
     3481 acccgcggcc gtgttgcagc caggtccgca ggtcgtcgct ggtctcgaac tcgaagctcg
     3541 cggtgaagtc gggcggcccc gaccacgcga ggcgcagggt gtgtggtggc gagccgggcg
     3601 cgtgctcgcc gaggtcgatc aaccagccgg gtgagcctgc cggatcgggc ggcgcgccag
     3661 cgaggcgctt gacgctgatc tgctcgcccg cgaccgtgac ctcgatcggt gacggccact
     3721 cgccgccgcg caccgggcgg gtcggcagcg ggtagagcgt cgcggagcgc tcgaggtcgg
     3781 cggtcacttg ccgctcacgc ggcatccgca gcgcgaacgg gaccggttcg ccgagcgcgt
     3841 cgacgatctt caggtcgcgc aggtcgggct gcagcgtgtg tgcatatgcg ccggcgggca
     3901 gctccaggta cacgaagggc gcggcctgga gcacctcgat cggcgcgcgc cagcgatagg
     3961 tcgcgtcggc ctcgcctgcg cgcgccgtgg ccgacccttg cagcgcgccc acgatcacga
     4021 cgatcgccga ccaatcattt ctccggtttc tccgcatggg gcctcccggt cgccggcagg
     4081 ggcgcgacga agccgatgac gagcatcagc acgccgacgc cgataaacga gatgatccgc
     4141 gtgaccgtgt ccgaggccgc gaggtcgacc gccagcagct tgacgatcac gagcgcgagc
     4201 agcccggcgc cgaccatcca cggcgcccgc gcggcgcggc gcaccgcgat ccacatgacg
     4261 atcagcgcga gcacggccca cagcagcgtg aagccggtct gcaccgcctg cgacgcggcc
     4321 atcgtgtcgg cgtcgaaggg cacgccgccg tggtgatgga acccgcggac catcgccgcg
     4381 ttgagccaca ggaagccgac ggcccagaac atccatcgca atgcatcgac gctgtcgccg
     4441 agcgtgtccc ggaccgcgtc gccgcggatc cacagccacg cggcgagcag cgcgacgccg
     4501 accccgaggt cgagcgggct gagcagcggc acgtaagtga gcggcgcagc ggacccgttc
     4561 gacacgaagt tggcggcgag gatccacagg aacagcccgg ccgttagcac cccgccggcg
     4621 atcgctcgat acgctaccgg ggcggcgcgc accggccaga cccgcagcgc agccgggcgc
     4681 gtcaggagca gcagcagcag cgcgggcacg gcgagccagc cgagccacgc ccacgcgctg
     4741 tcgacgtcgc cccacgcccg cgtgatcgcg cgaccttgca ggctgccgag cgccgccagc
     4801 accagggcac cggtcgcgtg cgcgacggtc tggccggcgg tcgaccagcg cggcgcggcg
     4861 cgccacagga ccacgacgtg ggtgagcagc gccagcggcc acgcccacca gccgccccac
     4921 gcacccgggc tcgtgaacgc ctcggcgctg acggccacgc acagcgcgag cacgggtgcg
     4981 tgggcgagga ctggcgcggt gatgttcttc cagcgcagcg cgtcggccag cgcgacgtac
     5041 gacagggcga tcgcgctcac ggtcgtcaac caggccgcga gccagtgcct cgactcgagc
     5101 acgaacgcgt cgatctcgac ccccgccgcg acgagcagcc acaacgtgcc ccacgcgatc
     5161 aggaacggct cgagcacgcg ctcgttgctc ttcgtcgcgg cggtgaagcg ctgcacgaag
     5221 tacgccgacg cgagcgcgcc gacggcgagc agcacgctgg cgaacaggtg cccgttggcg
     5281 atctcgaccg gtgtcggcag gcggtcgaac gcgtgcagca gcgggccgca ggcgagcagc
     5341 agcaggacat agccgcagac gcgggacacg acgcgctgct ggcgaaagcc gagccacacc
     5401 aggccggcgg cctcgagcgc ccacgcgccg gcggtgctgc gggcgtcgag ggcgaacggc
     5461 accacgaggg tgaggaacac cgtgccgatc gtcagcaccg cctcgaaggt cgcggcccag
     5521 cgcgggcgcg ggcgcatgta cgcggccatc gccacgtaga aggccgccag cgccagggcc
     5581 gagagcgcga cgccgaagtc gtcgccttcc accaggccgt attgcagcgc gaaggtgatc
     5641 gtcggcaggc cgaacagcag gctgccgttg agccacacca cgccgagcgt cgggtgctcg
     5701 tgcttgccgt cgaggtgccg cgccggcatc agcatgatcg cgatgaacag caggaagtac
     5761 gtgatcagga acgcttcact gctggcgaag tgctggggtt gatacttgag cacgccccac
     5821 gcgctgccga ccaggaacg
//
LOCUS       DASWNJ010001770         3296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_102743, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001770 DASWNJ010000000
VERSION     DASWNJ010001770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102743"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VGB85_29595"
     CDS             complement(<1..675)
                     /locus_tag="VGB85_29595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138289.1"
                     /translation="MRGVTVSSALAAMLVMSVVPAEADACTGIERPSPGLSLTGMETE
                     RTIAADGVLVFDAFARQTTPEAAVKAFALTMTFGGVAVPGTLEVVPLWSGILEYAGGV
                     EEGALPLHEFLIVWRPDAVLQPGTYVGLVEQTPEGGVVEHIDLTITVSGAVGPAIAAP
                     EVERVVAQEFIAEVHEQACCERTPGSCGDAPYCRPTAVRYETGLAIDAALAEAQRERA
                     YLWVAPV"
     gene            complement(663..1922)
                     /locus_tag="VGB85_29600"
     CDS             complement(663..1922)
                     /locus_tag="VGB85_29600"
                     /inference="COORDINATES: protein motif:HMM:NF025670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HEY0138290.1"
                     /translation="MRRLALFSACALAAGTARAEPVVQSEHANDQFGTAVVIEGDRMV
                     VGAPGANDGAFDAGAVHVFGRDGGEWRREAVLTADEPVPHGAFGGFLALRGDVLAIGA
                     PMQSGHEMKERHGTSYIFERVEGAWEQRLALRLEDVDADTFGVGVAIGVERAAFSAVS
                     RRRDETRLSGVVVVVVRDGDGWREEARLEDGAYPAFGLTLAMDDEHLIVSDGTGAALA
                     YRREGAQWVGGEALALPADAKLGLPTVRLSGAWLVLGAGYASPLYFFRRTGTTWEPTQ
                     VLDLGVPRLFAALALDGGRAAAGVDAGDMDMPYRHEVVVLERAGDRWEVSARVSGSNE
                     PGTAFGEALSLSAGRLAVADPDAGAFAGQRVGEVRIYAGPTWSEDAVVSPDSIDDAGC
                     GCRSREGSLAGLGWLVLLGWRRRRCAE"
     gene            2160..3215
                     /locus_tag="VGB85_29605"
     CDS             2160..3215
                     /locus_tag="VGB85_29605"
                     /inference="COORDINATES: protein motif:HMM:NF020269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GON domain-containing protein"
                     /protein_id="HEY0138291.1"
                     /translation="MNLVSRTLLSAAPWLLLACDIPDPSTDPAILAEDAADQAVLDAP
                     DRLQDAPADPDESLSCILCVLGGNHPTCGADGKSHPNACFAACAGGGAVPREDYWPDA
                     DADGFGDASATPTSACKAPAGTADNDEDCDDTADQVFPGNAEACDGLDNDCMPATECD
                     PVSCADIALEDPSAVDGSYTLHVGLDPARPWTAYCHDMAGDPASYLSLVAVGEGSNFS
                     QYASTPTVHTEFSRVRIDPATLAVDIDDLTFSTSTGATYHDGEVVTQMPYGVAESCSE
                     LNGAANIDLTGTPFRIATPFCTGGNVATGGAILSADGQVADITGSGSCGWTSPSCTYA
                     PHELNNGPVLELAYIDP"
BASE COUNT          498 a         1420 c          969 g          409 t
ORIGIN      
        1 cacgggcgcc acccacaggt acgcgcgctc gcgctgcgcc tccgccagcg ccgcgtcgat
       61 cgccaggccc gtctcgtaac gcacagccgt cggccggcag tacggcgcgt cgccgcagct
      121 gccgggcgtg cgctcgcagc aggcctgctc gtggacctcg gcgatgaatt cctgcgccac
      181 gacgcgctcg acctccggcg ccgcgatcgc gggccccacc gccccgctga ccgtgatcgt
      241 cagatcgatg tgctcgacga caccgccctc gggcgtctgc tcgacgagcc cgacatacgt
      301 gcccggctgc aagaccgcgt cgggccgcca cacgatcaaa aactcgtgca gcggcagggc
      361 gccctcctcg accccacccg cgtactcgag gatccccgac cacagcggca ccacctcgag
      421 cgtgcccggg accgccaccc cgccgaacgt catcgtcagc gcgaacgcct tcaccgccgc
      481 ctccggcgtc gtctgccgcg cgaacgcgtc gaacaccagc accccgtcgg ccgcgatcgt
      541 tcgctcggtc tccatgcccg tcaggctgag ccccggcgac ggccgctcga tccccgtgca
      601 cgcgtccgcc tccgccggca ccaccgacat caccagcatc gctgccagcg cgctgctcac
      661 cgttactccg cgcatcgtct ccgcctccat ccgagcagca ccagccaacc gaggccggcg
      721 agcgacccct cccgcgagcg acacccgcag cccgcatcat cgatcgagtc gggcgacacc
      781 acggcgtcct ccgaccacgt cggcccggcg tagatcctca cctcgccgac gcgctggccc
      841 gcgaacgcgc ccgcgtccgg atcggcgacc gcgaggcgcc ccgccgacag cgacagcgcc
      901 tccccgaacg ccgtccccgg ctcgttcgac ccggacaccc gcgcgctcac ctcccagcga
      961 tcgccggccc gctcgagcac cacgacctcg tgccgatacg gcatgtccat atcccccgca
     1021 tcgacgcccg ccgccgcccg cccgccgtcg agcgcgagcg cggcgaacag ccgcgggacg
     1081 ccgaggtcca gcacctgcgt cggctcccac gtcgtccccg tgcggcgaaa gaagtacagc
     1141 ggcgacgcat agccggcgcc gagcaccagc cacgccccgg acagccggac cgtcggcagc
     1201 cccagcttcg cgtccgccgg cagcgcgagc gcctcgcccc cgacccactg cgcgccctca
     1261 cgacgatatg ccagcgccgc gccggtccca tcgctgacga tcaagtgctc gtcgtccatc
     1321 gccagcgtca agccgaaggc agggtacgcc ccgtcctcga gccgcgcctc ctcgcgccag
     1381 ccgtcgccgt cacggaccac cacgaccacc acgcccgaca gccgcgtctc gtcgcgccgc
     1441 cggctgaccg ccgagaacgc cgcccgctcg accccgatcg ccacgccgac cccgaaggtg
     1501 tcggcgtcga cgtcctcgag ccgcagcgcc agccgctgct cccacgctcc ctcgaccctc
     1561 tcgaagatgt aagaagtccc gtggcgctcc ttcatctcgt ggcccgactg catcggcgcg
     1621 ccgatcgcca gcacatcccc gcgcagcgcc aggaacccgc cgaaggcccc gtgcggcacc
     1681 ggctcgtcgg cggtcaacac cgcctcgcgt cgccactccc cgccgtcgcg gccgaacacg
     1741 tgcaccgcgc ccgcgtcgaa cgccccgtcg ttggcccccg gcgccccgac caccatgcgg
     1801 tcaccttcga tcaccaccgc ggtgccgaac tgatcgttcg cgtgctccga ctgcaccacc
     1861 ggctccgcgc gcgccgtccc ggccgccaga gcgcacgcac taaacagcgc cagacgtctc
     1921 accggcgcac gatacccgat ccacccgccc gacagccacg acccgcaccg ggtccggcca
     1981 caccacaacc ggccgccccg gtccctggcg ccgagtgccg cagcgaggtc gagcgtcgcg
     2041 ggcctggtgc tcgctcgcgc gtgaaattcc cgtgaaatcg ccggtccccc gcgacccact
     2101 acgatggctc gaacaaaccg gcacgccggc ctcgccaccc tctcctctgg accaccgcaa
     2161 tgaacctcgt gtcacgcacc ctgctgtccg ccgccccctg gctgctcctc gcctgcgaca
     2221 tccccgaccc ctccacggac cctgccatcc tcgccgagga cgccgcggac caggccgtgc
     2281 tcgacgcccc ggaccggctt caagacgcgc cggccgaccc cgacgagtcg ctctcgtgta
     2341 tcctttgtgt gctcgggggc aaccacccga cctgcggcgc ggacggcaag agccacccca
     2401 acgcctgctt cgcggcctgt gcgggcggcg gcgccgtgcc ccgcgaggac tactggcccg
     2461 acgccgacgc cgacggcttc ggtgacgcga gcgcgacgcc gacgagcgcg tgcaaggctc
     2521 cggccggcac cgccgacaac gacgaggact gtgacgacac cgcggaccag gtgttccccg
     2581 gcaacgccga ggcgtgcgac ggcctcgata acgactgcat gccggccacc gaatgcgacc
     2641 cggtgagctg cgccgacatc gccctcgagg acccctctgc cgtcgacggg tcgtacacgc
     2701 tgcatgtcgg cctcgacccg gcgcggccgt ggaccgcgta ctgccacgac atggccggcg
     2761 acccggccag ctatctgtcg ctggtcgccg tcggcgaggg ctccaacttc tcgcaatacg
     2821 cctcgacccc gacggtccac accgaattct cgcgcgtccg gatcgacccg gccacgctcg
     2881 cggtcgacat cgacgacctg accttctcga cctccaccgg cgcgacctac cacgacggcg
     2941 aagtcgtcac gcaaatgccg tacggggtcg ccgagtcctg ctcggagctc aacggcgccg
     3001 ccaacatcga cctgaccggc acgccgtttc gcatcgccac cccgttttgc accggtggca
     3061 acgttgccac tggcggcgcg atcctcagcg cggacggcca ggtcgcggac atcaccggca
     3121 gcggctcctg tggctggacg agcccgagct gcacttacgc cccccacgag ctcaacaacg
     3181 gcccggtgct cgagctcgcc tacatcgacc cgtgagtcac atcttcttgt cgcccggcag
     3241 cagcccgatc tcgatcagcc gctgcgtcag gtactcgttc gcggtgatcg gctcgg
//
LOCUS       DASWNJ010001771         2011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_136070, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001771 DASWNJ010000000
VERSION     DASWNJ010001771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136070"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1479)
                     /gene="sufB"
                     /locus_tag="VGB85_29610"
     CDS             complement(46..1479)
                     /gene="sufB"
                     /locus_tag="VGB85_29610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEY0138292.1"
                     /translation="MTEQIVQPVVTQEEHLAALGKYEYGWADADVAGATAQRGLSEAV
                     VRNISALKNEPQWMLDLRLKGLRLFDRKPMPNWGADLTGIDFQNIKYFVRSTEKQAAS
                     WDDLPADIKNTYDKLGIPEAEKQRLVSGVAAQYESEVVYHKIREDLEEQGVLFLDTDT
                     ALREHEDIFKEYFGTVIPVGDNKFAALNTSVWSGGSFIYVPKGVHVDIPLQAYFRINT
                     ENMGQFERTLIIVDEGAYIHYVEGCTAPIYSSDSLHSAVVEIIVKKNARCRYTTIQNW
                     SNNVYNLVTKRATCEEGATMEWVDGNIGSKVTMKYPAVYMTGAHAHGEVLSVAMAGEG
                     QHQDAGAKMVHAAPYTSSTIVSKSIARGGGRTSYRGLVQVMEGSHHSKSTVKCDALLV
                     DTISRSDTYPYVDIREDDVSMGHEATVSKVSEDQLFYLMSRGLSEQQAMSMIVNGFIE
                     PVTRTLPMEYAVEWSRLIELNMEGSVG"
     gene            complement(1476..>2011)
                     /locus_tag="VGB85_29615"
     CDS             complement(1476..>2011)
                     /locus_tag="VGB85_29615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138293.1"
                     /translation="ETRSQGPRGRGRPAKAFVLTAAAREGFPHFYDGIATAALRWIAE
                     HGGPEAVTAFATSQVTGLEERCRTAMLEAGDDPIARAEALAEALTAEGYAANATTIAS
                     GGQLCQHHCPVAHVAAEFPQLCDAETAVISRLIGTHVQRLATIAHGDGVCTTHIPTPP
                     RAISTTVTTATTVRTDK"
BASE COUNT          282 a          652 c          704 g          373 t
ORIGIN      
        1 ctcgtgcttg ccgagcagat ccttggcctt gtcgaggaag cccacctagc cgaccgaccc
       61 ctccatgttg agctcgatca gccgcgacca ctccacggcg tactccatgg gcagggtgcg
      121 ggtgacgggc tcgatgaagc cgttgacgat catgctcatg gcctgctgct cgctgaggcc
      181 ccggctcatc aggtagaaga gctggtcctc gctgaccttg gagacggtgg cctcgtgccc
      241 catcgacacg tcgtcctcgc ggatgtcgac gtacgggtag gtgtccgacc gcgagatggt
      301 gtcgaccagc agggcgtcgc acttgaccgt gctcttgctg tggtgcgagc cctccatgac
      361 ctgcacgagc ccgcggtacg aggtgcggcc gccgccacgg gcgatcgact tcgacacgat
      421 cgtcgacgac gtgtacggcg cggcgtgcac catcttggcg ccggcgtcct ggtgctggcc
      481 ctcgccggcc atcgcgaccg acagcacctc gccgtgggcg tgcgcgccgg tcatgtagac
      541 ggccgggtac ttcatcgtca ccttggagcc gatgttgccg tcgacccact ccatggtcgc
      601 gccctcttcg caggtggcgc gcttggtgac caggttgtag acgttgttcg accagttctg
      661 gatggtcgtg taccggcagc gggcgttctt cttcacgatg atctcgacga ccgcgctgtg
      721 cagcgagtcg gacgagtaga tcggggcggt gcagccctcc acgtagtgga tgtacgcgcc
      781 ctcgtcgacg atgatcagcg tccgctcgaa ctggcccatg ttctcggtgt tgatccggaa
      841 gtaggcctgc agcgggatgt cgacgtgcac acccttcggc acgtagatga aggagccacc
      901 ggaccacacg gaggtgttca gcgcggcgaa cttgttgtcc ccgaccggga tgaccgtgcc
      961 gaagtactcc ttgaagatgt cctcgtgctc acgcagcgcc gtgtcggtgt cgaggaacag
     1021 gacgccctgc tcctcgaggt cctcacggat cttgtggtag acgacctcgg actcgtactg
     1081 ggccgcgaca ccggagacga ggcgctgctt ctccgcctcg gggatgccga gcttgtcgta
     1141 cgtgttcttg atgtccgccg gcaggtcgtc ccaggaggcc gcctgcttct cggtggagcg
     1201 cacgaagtac ttgatgttct ggaagtcgat gccggtgagg tcggcgcccc agttcggcat
     1261 gggcttgcgg tcgaacaggc gcaggccctt gagccgcagg tcgagcatcc actgcggttc
     1321 gttcttcagt gcggagatgt tgcgcaccac ggcctcggac agcccacgct gggcggtcgc
     1381 gcccgcgacg tcggcgtcgg cccacccgta ttcgtacttg cccagggcgg cgaggtgctc
     1441 ttcctgagtg acgacgggct ggacgatctg ctcggtcatt tatctgtcct cacagtggtg
     1501 gcggttgtaa cggttgtgct gatcgcacgc ggcggggtgg ggatgtgcgt ggtgcacacc
     1561 ccgtcaccgt gcgcgatggt ggccaggcgc tgcacgtggg tgccgatgag ccgcgagatg
     1621 accgcggtct cggcgtcgca cagctggggg aactcggcgg cgacgtgcgc caccgggcag
     1681 tggtgctggc acagctgccc gccggacgcg atcgtcgtcg cgttggcagc gtaaccctcg
     1741 gcggtcaggg cctccgcgag tgcctccgcc cgcgcgatcg ggtcgtcgcc ggcctccagc
     1801 atcgcggtcc ggcagcgctc ctccaggccc gtgacctgag aggtggcgaa ggcggtgacc
     1861 gcctccgggc cgccgtgctc ggcgatccag cgcagggcgg cggtggcgat gccgtcgtag
     1921 aagtgcggga aaccctcccg ggcggccgcg gtgagcacga aggccttggc gggccggccc
     1981 cgtccgcgcg gaccctgcga ccgggtctcg c
//
LOCUS       DASWNJ010001772         4257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_383110, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001772 DASWNJ010000000
VERSION     DASWNJ010001772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383110"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1354)
                     /locus_tag="VGB85_29620"
     CDS             complement(56..1354)
                     /locus_tag="VGB85_29620"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY0138294.1"
                     /translation="MNARTIRNVAVIAGARTPFCKAGGAFIRRSAVQLGAVAARETML
                     RAGIRPDRVEQIIFGIVSAPVGAPNIAREIGLEAAFPTNVPAYTISRACISSHTAVTS
                     AADQIALGRSDVILAGGAEVLSDVPILYGRKFRDALFVASKARTMGDRLRAFSTVRLA
                     DLAPVAPAIAEPSSGQTMGQAAEKMAKDFGIPRAAQDRFAANSHQRAVAAWKAGAFAQ
                     RVAPVPVPKGRELGIVERDDHPREDTTEEKLAKLKPVFDRELGSVTAGNASPLTDGAS
                     AVLLAAEEVARAEGWPILGILRGYHVTADDPFEHLLMGPVGAIASVLNQTGLGLADMN
                     VIEMHEAFAAQVLSNIHGLGSAKYCAEKLGRSTAVGEIDPEFVNKWGGSISLGHPFGA
                     TGGRMVLQLLDQLQASGGQFGLISACAAGGMGAAMVFERV"
     gene            complement(1351..3543)
                     /locus_tag="VGB85_29625"
     CDS             complement(1351..3543)
                     /locus_tag="VGB85_29625"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY0138295.1"
                     /translation="MFTLTLDPPEVSPRLAIITIDRPGEKVNTIAPAALEQVEQQFAE
                     IENTPGLAGIVLISGKPDTFIAGADVEVFATAKERRDVEAFGRRGHEVLARIAAAKVP
                     IVAAIHGACLGAGLELTLACTYRIATDSPKTVLALPEVQLGLFPGGGGVSRLPRLIGL
                     REALDMILTGRNIRPRAAKRMGLVDQVVAEESLLPAARAAAAKLAAGKLQPRLHPKGD
                     VMRALLEHNPLGRSIVFRQARKTIEKKTFGLYPAPLVALDLIEKGLRLPLDEALKLEP
                     PAFAELAAGATSRSLVSLFRRSNALKKQPVFDADGARVEGAEIRRLGMVGGGFMGADI
                     AVVSAEKGIETRIRDIAAEPLGKALAHCKAHFDRKARSVGERHVFKARSRVSGGLDLT
                     GFNTMDLIIEAVPEVLSLKQEVFAQLEVMVPRTTILASNTSALPITRIAERMQHRDRV
                     IGLHFFSPVSKMPLLEIVKTAETSPQTLATCLRYAAQIGKTPIVVNDGPGFYTTRVLG
                     FYLMAALEMVQAGHSIEAVDRGARMVGWPVGPITLLDEVGIDVGAKVSKTMAAAFPDR
                     IKVPDTVDQFLAEKRLGRKSGRGFYAYPTEKRGLLARKARKHVDPGVYGYFAGRQAIA
                     QEGNPEELGERLTLIAAMEAVRCLESGILQSPRDGDIGAVFGFGYPPMRGGPFRHIDA
                     LGPGAVLTRLAVLRERHGDAYAAPELLVRLAREGKNFASLGSPEGDHA"
     gene            complement(3756..>4257)
                     /locus_tag="VGB85_29630"
     CDS             complement(3756..>4257)
                     /locus_tag="VGB85_29630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138296.1"
                     /translation="DSDASPAAHSATRTTVRAVPPISVEEPPDRLGSALRWWTVVSTT
                     CTSMVFVFAFASLLRRAESRPALDLESDEAAVVDTSRAPPPGPALPVMPPEPAAVPEP
                     VAAPEPAASPEPIVLPPPEAEPPVRELPPQRDPPPVSKPRRPRPPKPVEDDPGELMPI
                     RPRGNK"
BASE COUNT          584 a         1462 c         1610 g          601 t
ORIGIN      
        1 cttgccgggc accgcacacc cggacccccg cgaggcaaat tccggccgaa cgctctcaga
       61 cccgctcgaa caccatcgcc gcccccatgc cgccggcggc gcaggcggag atgagtccga
      121 actgcccgcc gctggcctgc aactggtcga gcagttgcag gaccatgcgg ccgccggtag
      181 cgccgaaggg gtgtccgagc gagatgctgc cgccccactt gttgacgaac tcggggtcga
      241 tctcaccgac cgcggtgctg cggccgagct tctccgcgca gtacttcgcc gagccgagcc
      301 cgtggatgtt gctgagcacc tgcgccgcga aggcctcgtg catctcgatg acgttcatgt
      361 cggcgaggcc gagcccggtc tggttgagca cgctggcgat cgcgccgacg gggcccatca
      421 gcaggtgctc gaaggggtcg tccgcggtga cgtggtagcc gcgcaggatg ccgaggatcg
      481 gccagccctc cgcgcgcgcg acctcctccg cggcgagcag caccgccgag gcgccgtcgg
      541 tcaacggcga ggcgttgccc gcggtcaccg agccgagctc gcggtcgaac accggcttga
      601 gcttggcgag cttctcctcg gtcgtgtcct cgcgcgggtg gtcgtcgcgc tcgacgatgc
      661 cgagctccct gcccttcggg acaggtaccg gcgcgacccg ctgcgcgaag gccccggcct
      721 tccaggccgc gacggcgcgc tggtggctgt tggcggcgaa gcggtcctgc gccgcgcgcg
      781 ggatgccgaa gtccttggcc atcttctcgg cggcctgacc catcgtctgc ccgctcgagg
      841 gctcggcgat cgccggggcg accggggcga ggtcggcgag gcgcacggtg ctaaacgccc
      901 gcaggcggtc gcccatcgtg cgcgccttgc tggcgacgaa cagcgcgtca cggaacttgc
      961 gaccgtagag gatcggcacg tcgctgagca cctcggcccc accggcgagg atcacgtcgg
     1021 agcggccgag cgcgatctga tcggcggccg aggtgaccgc cgtatgcgag gagatgcagg
     1081 cgcggctgat cgtgtacgca ggtacattgg tcgggaatgc cgcctcgagc ccgatctcgc
     1141 gggcgatgtt cggggcgccg accggcgccg agacgatgcc gaagatgatc tgctcgacgc
     1201 ggtccgggcg gatcccggcg cgcagcatgg tctcgcgcgc cgcgacggcg ccgagctgga
     1261 ccgcggaccg gcggatgaag gcaccacccg ccttacaaaa gggcgtgcgg gcgccggcga
     1321 tgacggcgac gttacggatc gtgcgggcgt tcatgcgtgg tctccttcgg ggctgccgag
     1381 gcttgcgaaa ttcttgccct cgcgggcgag gcggacgagc agctccgggg ccgcgtaggc
     1441 gtcgccgtgg cgctcgcgca gcacggcgag gcgggtcaac acggcgccgg ggccgagcgc
     1501 gtcgatgtga cgaaacggcc cgccgcgcat cggcgggtag ccgaagccga acacggcccc
     1561 gatgtcgccg tcgcgcgggc tctgaaggat cccggactcg aggcagcgca cggcctccat
     1621 cgcggcgatc agcgtcaggc gctcgccgag ctcctcgggg ttgccctcct gcgcgatcgc
     1681 ctggcgtccg gcgaagtagc cgtagacgcc cggatcgacg tgcttgcggg ccttgcgagc
     1741 cagcaggccg cgcttctcgg tcgggtacgc gtagaagccg cgacccgact tgcgaccgag
     1801 gcgcttctcc gcgaggaact ggtcgaccgt gtccgggacc ttgatgcggt ccgggaacgc
     1861 ggcggccatc gtcttgctga ccttggcgcc gacgtcgatg ccgacctcgt cgagcagcgt
     1921 gatcggcccg acgggccagc cgaccatgcg cgcgccgcgg tcgacggcct cgatgctgtg
     1981 cccggcctgg accatctcga gcgcggccat cagataaaac ccgagcacgc gcgtggtgta
     2041 gaagcccggg ccgtcgttga cgacgatcgg ggtcttgccg atctgcgccg cgtagcgcag
     2101 gcaagtggcg agcgtctgcg gcgaggtctc ggccgtcttg acgatctcga gcagcggcat
     2161 cttcgacacc ggcgagaaga agtgcaggcc gatcacgcgg tcgcggtgct gcatgcgctc
     2221 ggcgatccgg gtgatcggca gcgccgaggt gttgctggcg aggatcgtcg tccgcgggac
     2281 catcacctcg agctgcgcga agacctcctg cttgaggctc aggacctccg gcaccgcctc
     2341 gatgatcagg tccatcgtgt tgaagccggt caggtcgagg ccgccgctga cgcggctgcg
     2401 ggccttgaac acgtggcgct cgccgacgct gcgggccttg cggtcgaagt gcgccttgca
     2461 gtgcgcgagc gccttgccga gcggctcggc ggcgatgtca cggatccgcg tctcgatgcc
     2521 cttctcggcc gagaccaccg cgatgtcggc gcccatgaaa ccaccaccga ccatgccgag
     2581 gcgacggatc tccgcgccct cgacccgcgc gccgtcggcg tcgaacaccg gctgcttctt
     2641 gagcgcgttg ctgcggcgaa acagcgagac cagcgagcgc gaggtcgccc cggcggccag
     2701 ctcggcgaag gccggcggct cgagcttgag cgcctcgtcg agcggcaagc gcaggccctt
     2761 ctcgatcagg tcgagggcga cgagcggcgc ggggtacagg ccgaaggtct tcttctcgat
     2821 cgtcttgcgc gcctgacgaa agacgatcga gcggccgagc gggttgtgct cgagcagcgc
     2881 gcgcatcaca tcacccttcg ggtgcaggcg cggctgcagc ttgccggccg cgagcttggc
     2941 cgccgccgcg cgagccgcgg gcagcagcga ctcctcggcg acgacctggt cgaccaggcc
     3001 catgcgcttg gcggcgcgcg ggcggatgtt gcgaccggtg aggatcatgt cgagcgcctc
     3061 ccgcaggccg atcagccgcg gcagtcgcga cacgccgccg ccgcccggga acagcccgag
     3121 ctgcacctcg ggcagcgcga gcacggtctt gggcgagtcg gtggcgatcc ggtaggtgca
     3181 ggcgagcgtg agctcgaggc ccgcgccgag gcacgcgccg tggatcgcgg cgacgatcgg
     3241 gaccttggcg gcggcgatcc gcgcgagcac ctcgtgacca cgacgcccga aggcctcgac
     3301 gtcgcggcgc tccttcgcgg tcgcgaacac ctcgacgtcg gcgccggcga tgaaggtgtc
     3361 gggcttgccg gagatcagga cgatgccggc gaggcccggc gtgttctcga tctcggcgaa
     3421 ctgttgttcg acctgctcga gcgcagcggg ggcgatggtg ttgaccttct cgcctggccg
     3481 gtcgatcgtg atgatcgcca ggcggggcga gacctccggt gggtcgagcg tgagcgtgaa
     3541 catggcggcg cggacattgg cgcgcctgta gcgcccgcgc aaggagaaag tgcgtgcgcg
     3601 ggccaccagg ggcccgcgag ggcgacgtca tgtcgcgtgg cagccccacc tcgcggtggc
     3661 cgcccggccg cgccacgcct cgcgtcgtgg tcgcggcagc gccggtccgg gattaccctg
     3721 acacggtcgc gcgaccgcgc atcgtcgccg cggcgtcact tgttgccgcg ggggcggatc
     3781 ggcatcagct cgccggggtc gtcctcgacg ggctttggcg gcctcggcct gcgtggcttg
     3841 ctgaccggcg gcgggtcgcg ctgcggcggc agctcgcgca ccggcggctc ggcctccggc
     3901 ggcggaagaa cgatcggctc gggcgatgcg gcgggttccg gtgctgccac gggctcgggc
     3961 accgccgcgg gctccggcgg catgacgggc agagcggggc ccggcggggg cgcgcgcgag
     4021 gtgtcgacga cggcggcctc gtccgactcg aggtcgagcg cgggcctcga ctcggcgcgt
     4081 cgcagcagcg aggcgaaggc gaacacgaag accatcgagg tgcaggtggt cgagacgacg
     4141 gtccaccagc gcagcgccga gccgaggcgg tcgggcggct cctcgacgga gatcggcggt
     4201 acggctcgca cggtcgtgcg tgtcgcggag tgggccgcgg gcgaggcatc ggagtcg
//
LOCUS       DASWNJ010001773         7632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_383123, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001773 DASWNJ010000000
VERSION     DASWNJ010001773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7632
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383123"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..1238)
                     /locus_tag="VGB85_29635"
     CDS             complement(102..1238)
                     /locus_tag="VGB85_29635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138297.1"
                     /translation="MDERGRLCVCSAHAGVSTSLSPQIGERFAGRYELKSPLRSDGVV
                     RAFLAFDHHAGLDVALLLFEPACAHPTAWGAFARIVAAASAGKVAGLVLPQGVGGTPP
                     VPPFCLAEPQALRGFDRLRDQGMMPWQRALTLGERAAEILHAAHIATGVAHRVLTPSR
                     CAVTVRDDVRVLDFGVAELELGRPDEAGYRAPEQQQGGGDTRSDVYSLAVILFELISG
                     QRSAGKPPQSLRSLVPVPRPVDDLMAKALAQDPAQRLDLAAMRAALRELLGMAPAPAE
                     ATHGVVAGTKPASSPVAIVSAPPGTSVAPSVTTPVRSEYAVGAGSTPANVLTPPARPS
                     APPAAPPAAPLSPRPEVAPPRRSPAVAPWGGPVLAQRERDRWVG"
     gene            1375..2376
                     /locus_tag="VGB85_29640"
     CDS             1375..2376
                     /locus_tag="VGB85_29640"
                     /inference="COORDINATES: protein motif:HMM:NF026876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HEY0138298.1"
                     /translation="MPDAARISDFHVCPKVEPGPVPHVGGPIFSGSANVIIGFLPAAR
                     EGDSIVCFPIGPTDQIKQGSASVLINGRPAARRSDPGSHISGDLIAAGCPTVVIGDSN
                     QAFTFRAAARRGTPFCEECERKRREMEDRDDSAEVAAPDPDTATLDDDQPPPGADLGR
                     DLLASSLLNKQSLAGQPDQGDGLDVERRAARLAVAYQFYAAHAGAKIKPSKITSHIRA
                     IDISQPVEVMSVAGQTLYQRGYPGGDNGQYFALDPGVTPEQLGTSSLVPGPGGDPIVR
                     DSRTVGFGEATAFGLKSTAAPVGDTWTFRDSGEVVACEGGGTQVFVPQPFHVSSRNL"
     gene            2387..3514
                     /locus_tag="VGB85_29645"
     CDS             2387..3514
                     /locus_tag="VGB85_29645"
                     /inference="COORDINATES: protein motif:HMM:NF024894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein Tsi6 family protein"
                     /protein_id="HEY0138299.1"
                     /translation="MADLMRILSRDAFAEVLGEARRRTRALDPSRWPLLQQIEAQLDF
                     IASKTENGRVPHEDDRHRTTFGSLAARNLEDVDPEYADQLEELDYTFRRYPQLPAGPP
                     VRRRGVLQVWSGRETYRKLVLDLGVPRTVGSALADFIVHGDSAGSPHFQIVWDGVSAH
                     VQAEGSHRLTIAGQPAWYGEMANRGWMTAVRTTYRFLVEDRSPPPEPVKPSAASLSAL
                     TELERRCDTGNLYAILDAARSDRALQLVEESIDPYASLYDGEQGRAFDDIAPYLVHLQ
                     PSSWLLERLVTEGWDDAWGIYVESSAGFDKVRRHFRQFLKVEVEAEAKPLFFRFYDPR
                     VLGTFIEVITPEQRSDFMSVLDALIFADRESHLRRFGKTSV"
     gene            complement(3569..5674)
                     /gene="tssI"
                     /locus_tag="VGB85_29650"
     CDS             complement(3569..5674)
                     /gene="tssI"
                     /locus_tag="VGB85_29650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HEY0138300.1"
                     /translation="MSSEQPFTLEIEGAPLRVVRFAGEEALSSLFDFRIEVASPDLDP
                     LDLLAKPVLLTIRGAEAPRLVHGLVYEAEYVGFTRQLQLYSLKVGPWANLLTYRSNCR
                     IFQDKTTQQIVTEVLTKAGLPSDWLRFSLTSSYAPRNYCVQYRESDFVFVSRLLEEDG
                     IFYFFEHQEDKHVWVMADGAQAHTPIAGTPTVWFSPPIGVVQDREHVKTFRLGNRVRT
                     GAVSLRDFNLHKPAQAMEVKATAKANPELEAYDFPGEYQEPGRAGPHEGQTLARIRLE
                     ALQAARKSGGGESDCARLTAGQTMLLAGHPQHELNAEYRLVQVSHRGEQPQVLEQDAS
                     GASSYHNTFVVTEIKVPFRPARVTERPTVRGLQSAEVVGPEGEEVHTDEHGRVRVQFH
                     WDREGKLDEHSTCWVRVSQLWAGSGWGAMFLPRIGHEVLVDFLEGDPDRPIITGRIYH
                     GQNATPYPLPDQKTKSTIKSESSPGGGGYNELRFEDLKEKEQVFLHAERNLDVRVNHD
                     AFTTVSNDSHVTVGHEEEGKAGDSFVQLFHDQHLKVHRHTKAHLGGDVELLVGGVDGP
                     GRVDVHVRSDKLELVDGDRHEHVRKSAFEKIDGSVSRIVEGNEQTKIAGRIAIEAGDE
                     VHLKASKIVLDAGSALTIQGPGGFITINSSGIYIKGNMVYINSAGAPVAAQDAAPGTA
                     QDAKDAAPIAATPSERGSK"
     gene            complement(5983..6231)
                     /locus_tag="VGB85_29655"
     CDS             complement(5983..6231)
                     /locus_tag="VGB85_29655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138301.1"
                     /translation="MRESELGQIRVTLHFEEDTVVVVLDFVEGNREERLSGPYRAEEG
                     WLVSPVVFKGKPIRVQQEGSFLVLHPPDEDEMVLVRIA"
     gene            complement(6277..6981)
                     /locus_tag="VGB85_29660"
     CDS             complement(6277..6981)
                     /locus_tag="VGB85_29660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138302.1"
                     /translation="MSGGQSHADCFSHDSIDSIKESCVASRRHVIVLSDRPVKNTLGT
                     LYHYSLEVWWSDHDVEIGKEYDNRDELSEACAKGGRLGGVELLRESGWRAYKYNRYPM
                     MGPPRWQEESVALSVIHYQPSPTEFVPLYQGSIPDVSKKWRSVSGEAANYAYAEQAGV
                     TRPQNWPNSIYLLGDDVNNSNTFIRHLVAKGGMTMKEMDGLHPGRMKPRADPVLYSAV
                     WRSDEAKPATPAEPKK"
     gene            complement(6978..7253)
                     /locus_tag="VGB85_29665"
     CDS             complement(6978..7253)
                     /locus_tag="VGB85_29665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138303.1"
                     /translation="MTLKRDCPEQYERRPESSTISWIENCIEYVRLHNIHSERYVYQF
                     VSVIFAHAEGPERLLGRDLEAAFYDGSLQRQLKNLEHCLRGTRGIDK"
BASE COUNT         1253 a         2769 c         2289 g         1321 t
ORIGIN      
        1 caggcccgcg agctccgtcg caagcgccga gaactggacc aactcggcgc cgccgcataa
       61 cttccttcaa cccttcacca cgccctcaac ccctccgtac gtcaacccac ccacctgtct
      121 cgttcacgct gagccaatac agggccaccc cacggcgcga ccgcgggaga ccgccgtggg
      181 ggagcgacct ccggtcgggg cgacagcgga gccgccggcg gcgcggcggg cggtgccgat
      241 ggccgtgccg gcggagtcag cacattcgcc ggagttgacc ccgcccccac cgcatactcg
      301 gatctcaccg gcgtcgtcac ggatggcgcc acgctcgtgc ccggcggcgc cgagacgatc
      361 gctaccgggc tgctcgcggg tttggtgccg gccaccacac catgggtcgc ctcggccggc
      421 gccggggcca tacccagcaa ctcccgcagc gccgcgcgca tcgccgcgag atcgagtcgc
      481 tgcgccggat cctgcgccag cgccttcgcc atgagatcat cgactggtcg cggcaccggg
      541 acgagcgagc gcaggctctg aggcggcttt ccggcggacc tctggcccga gatcagctcg
      601 aacaggatga ccgcgagcga gtacacgtcg ctgcgcgtgt cgccgccgcc ctgctgctgc
      661 tccggcgccc gatatcccgc ctcatccggg cggcccagct ccagttcggc cactccgaag
      721 tcgagcactc tgacgtcgtc gcgcactgtc acggcgcagc gcgacggcgt caggacccgg
      781 tgagcgacgc ccgtcgcgat gtgagccgcg tgcaggatct ccgccgcgcg ctcgccgagc
      841 gtcagggcgc gctgccacgg catcatcccc tggtcgcgca atctgtcgaa tccccggagc
      901 gcctgcggct cggccaggca aaacggaggc actggaggcg tgccgccgac cccctgcggc
      961 agcaccagcc ctgcgacctt tccagccgac gccgccgcga cgatccgtgc gaacgcgccc
     1021 cacgcggtcg ggtgcgcgca ggccggctcg aacaacagca gcgcgacgtc gaggcccgca
     1081 tggtggtcga acgccaggaa cgcccgcacc acgccgtcgc tccggagcgg cgacttgagc
     1141 tcgtagcggc cggcgaaacg ctcgccgatc tgcggtgaaa gcgaggtcga cacgccagcg
     1201 tgcgccgagc atacacataa tcgtccgcgc tcgtccattg aggtcctcga tgccaccgag
     1261 gaccctcgcg tccgccacct gttcgcctcc gcgcggcgcg accctggccg cgcttcgacg
     1321 ctcgcagagc gcgtccgtcc gccgcgtcct gcacatgata agatcgcggg atccatgccc
     1381 gacgccgctc ggatcagcga ctttcatgtt tgcccgaagg tcgagcccgg gcccgtcccg
     1441 cacgtcggtg gcccgatatt cagcggcagc gccaacgtga tcatcggatt tttgccggcc
     1501 gctcgcgagg gcgacagcat cgtctgcttc ccgatcggcc cgaccgacca gatcaagcag
     1561 ggctcggcga gcgtgctgat caacggccgc cctgccgccc gcagatccga tcccggatct
     1621 cacatatccg gggacttgat cgccgctggc tgtcccacgg tcgtgatcgg cgactccaac
     1681 caggccttca ccttccgcgc cgccgcccgg cgcggcacac ccttttgtga ggagtgcgag
     1741 cgcaagcgcc gtgagatgga ggatcgcgac gactccgccg aggtcgccgc gccggatccc
     1801 gacaccgcga ccctcgacga cgaccagccc ccgcccggcg ccgacctcgg ccgcgacctc
     1861 ctcgccagct cgctgctcaa caagcagagc ctcgccggtc agcccgacca gggcgacggc
     1921 ctcgatgtcg agcggagagc cgcccggctc gccgtcgcct atcagttcta tgccgcccac
     1981 gccggcgcga agatcaagcc cagcaagatt acctcgcaca tccgcgcgat cgacatctcg
     2041 cagccggtcg aggtgatgtc ggtcgctggc cagacgctct accagcgcgg ctaccccggc
     2101 ggcgacaacg ggcaatactt cgcgctcgac cccggcgtca cgcccgagca gctcggcacc
     2161 tcctcgctcg tccccgggcc cggtggtgac cctatcgtca gagatagccg caccgtcggc
     2221 ttcggcgagg cgaccgcgtt cggcctcaag agcaccgccg cccccgtcgg cgacacgtgg
     2281 acattccgcg actccggtga ggtggtcgca tgcgagggcg gcggcaccca ggtcttcgtc
     2341 ccgcagccct tccacgtctc cagccgcaac ctctgaggcg cacaccatgg ccgacctgat
     2401 gcggatcctt tcacgcgacg ccttcgccga ggtgctcggc gaggcccgcc ggcgcactcg
     2461 ggcgctcgac ccctcccgct ggccgctgct gcagcagatc gaggcccagc tcgacttcat
     2521 cgcctcgaag acggaaaacg gccgcgtccc ccacgaggac gatcgtcatc ggaccacctt
     2581 cggctcgctc gccgcccgca acctcgagga cgtcgacccc gagtacgccg accagctcga
     2641 ggagctcgac tatacatttc gccgctaccc gcaattgccc gccggcccac ctgtgcgtcg
     2701 tcgcggcgtc ttgcaggttt ggtctggccg cgagacctac cgcaagctgg tcctcgacct
     2761 cggcgtgccg cgcaccgtcg gcagcgccct cgccgacttc atcgtccacg gcgactccgc
     2821 cggctccccg cactttcaga tcgtctggga cggcgtcagc gcccacgtcc aggccgaggg
     2881 ctcgcaccgc ctcacgatcg ccggccagcc cgcctggtac ggcgagatgg ccaaccgcgg
     2941 ctggatgacc gccgtgcgca ccacctaccg cttcctcgtc gaggaccgca gcccgccgcc
     3001 cgagccggtc aagccctctg ccgccagcct ctcggccctc accgagctcg agcgccgctg
     3061 cgacaccggc aacctctacg cgatcctcga cgccgcccgc tccgaccgcg ccctgcagct
     3121 cgtcgaggag agcatcgacc cctacgcctc gctttacgac ggcgaacagg gccgcgcctt
     3181 cgacgacatc gccccctacc tcgtgcacct gcaaccgagc tcgtggctgc tcgagcgcct
     3241 ggtcaccgag ggctgggacg acgcctgggg gatctatgtc gagagcagcg ccggcttcga
     3301 caaggtgcga cgccacttcc ggcaattcct caaggtcgag gtcgaggcgg aggcgaagcc
     3361 gctgttcttc cgcttctatg acccgcgagt gctgggcacg ttcattgaag tgatcacacc
     3421 cgaacagcgc tcggacttca tgagtgtcct ggatgcgctg atcttcgccg accgggagtc
     3481 gcacctgcgc aggttcggca agacctccgt gtgagcctcg cattccgctg caccaccggc
     3541 tcgtcaccgg cgtcacgttg agcggcttct acttcgaccc gcgctccgac ggcgtcgctg
     3601 cgatcggcgc cgcgtccttc gcgtcttgcg ccgtcccggg cgcggcgtcc tgcgctgcca
     3661 cgggtgcccc cgcgctgttg atgtacacca tgttcccttt gatgtagatc ccgctcgagt
     3721 tgatcgtgat gaacccgccg ggcccctgga tcgtcagcgc gctgccggcg tcgaggacga
     3781 tcttgctcgc cttgaggtgc acctcgtcgc cggcctcgat cgcgatccgg ccggcgatct
     3841 tcgtctgctc attgccctcg acgatccgcg acaccgatcc gtcgatcttc tcgaacgcac
     3901 tcttgcgcac gtgctcgtgg cggtcgccgt cgacgagctc gagcttgtcg ctgcggacgt
     3961 gtacgtcgac gcgcccgggc ccgtcgacgc cgccgaccag cagctcgacg tcgccgccga
     4021 ggtgcgcctt cgtgtgacgg tggaccttca ggtgctggtc gtggaacagc tgcacgaagc
     4081 tgtccccggc cttcccctcc tcctcgtggc cgaccgtcac gtgcgagtcg ttcgacaccg
     4141 tggtaaacgc gtcatggttg acgcgcacgt cgaggttacg ctccgcatga aggaagacct
     4201 gctccttctc cttgaggtcc tcgaagcgca gctcgttgta tccgccgccg cccggcgagc
     4261 tctcggactt gatcgtgctc ttcgtcttct ggtccggcag cggatacggc gtcgcgttct
     4321 ggccgtggta gatccggccg gtgatgatcg gtcggtcggg gtctccctcg aggaagtcga
     4381 ccagcacctc gtgaccgatc cgcggcagga acatcgcgcc ccacccgctt cctgcccaaa
     4441 gttgactgac gcggacccag cacgtgctgt gctcgtcgag cttgccctcg cggtcccagt
     4501 gaaattgcac ccgcacccgt ccgtgctcgt cggtgtgcac ctcctcgccc tcgggcccga
     4561 ccacctccgc cgactgcagc ccacgcacgg tcggccgctc ggtcacacgc gccggcctaa
     4621 acggcacctt gatctccgtc accacgaacg tgttatgata tgagctcgcc cccgacgcgt
     4681 cctgctcgag cacctgcggc tgctcgccgc gatggctgac ctgcacgagg cggtactccg
     4741 cgttgagctc gtgctgcggg tgcccggcga ggagcatcgt ctgccccgct gtcaggcgcg
     4801 cgcagtcgct ctcgccgccg ccggacttgc gagccgcctg taacgcctcg agccggatcc
     4861 tcgccagcgt ctgcccctcg tgcggaccgg cgcgaccggg ctcctggtac tccccgggga
     4921 agtcgtaggc ctcgagctcc gggttcgcct tcgccgtcgc cttgacctcc atcgcctgag
     4981 cgggcttgtg caggttgaag tcgcgtagcg acacagcccc ggtccggacg cgattgccga
     5041 ggcggaacgt cttgacatgc tcacggtcct gcaccacgcc gatcggcggg ctaaaccaca
     5101 cggtcggtgt ccccgcgatc ggtgtgtgtg cctgggcgcc gtccgccatt acccacacgt
     5161 gtttgtcctc ctgatgctcg aagaagtaga agatcccgtc ctcctcgagc agccggctga
     5221 cgaatacgaa gtcggactcg cggtactgca cgcagtagtt gcgtggagcg taggagctgg
     5281 tcagcgagaa gcgaagccaa tccgacggca gccccgcctt cgtcagcacc tcggtgacga
     5341 tctgctgggt cgtcttgtcc tgaaagatcc ggcaattcga gcggtaggtc agcagattgg
     5401 cccagggccc gacctttagc gagtacagct gcagctggcg cgtgaagccg acatactccg
     5461 cctcgtaaac cagcccgtgg accagcctcg gcgcctcggc gccgcggatc gtcaacagga
     5521 ccggcttcgc cagcaggtcg agcgggtcga ggtcggggct cgccacctcg atccggaagt
     5581 cgaacaggct cgagagcgcc tcctcgcccg cgaagcggac gacccgcagc ggcgccccct
     5641 cgatctccag cgtgaatggc tgctcggacg acaacagcgc ggatgcggca gagatagagt
     5701 cggcaataga catcggaata cgcggccaga gaaaaaccgc agctcatgat aggcgagcgg
     5761 gcgaacgagc gtcaacacgc ccgggccccg tgcgatctcg tgcgcgcgac cctgtgcgcc
     5821 gggtccggtc cctcgagcgg ccgccgcgcg accatcggcc cgtttccgct ttcccggggg
     5881 cgacggcccc tggcgcagcc gcccagcgcc tgcaaccttt tagcgtgccg accgcccagg
     5941 aagctcggcg acccgtcacg accgcacgct cgcgtagaac actcacgcga tgcgaacaag
     6001 gaccatctca tcttcatcag gagggtggag aacgagaaat gagccctcct gctggactct
     6061 gataggcttt cccttgaaaa cgacggggct gaccagccat ccctcctctg ctctataagg
     6121 gccagacaat cgctcctctc gattcccctc gacgaaatcg agaactacga ccaccgtatc
     6181 ctcttcgaag tgcaacgtca cgcgaatctg tccgagctcg ctctcgcgca caccggcttc
     6241 ccagcttcct tccaagtgca tattcacttt tgccaatcac ttcttcggct cagccggcgt
     6301 cgcaggcttc gcctcatcgc tgcgccacac cgcagagtat aacaccggat cggctcgggg
     6361 cttcatacgt cctgggtgta agccatccat ctctttcatc gtcatccctc ccttcgccac
     6421 caggtggcgt atgaatgtat tactgttatt aacatcatcc ccgagcaaat aaatcgaatt
     6481 gggccagttt tgaggcctgg taacaccagc ctgctcggcg tacgcataat tggcggcttc
     6541 tccactgacg gagcgccatt ttttactcac atcagggatg gagccttgat agagcggcac
     6601 aaactccgtg gggctgggtt ggtagtggat cacggacagg gcgacgctct cctcttgcca
     6661 acgcggaggg cccatcatgg ggtaacgatt atatttgtaa gcgcgccagc cactttcgcg
     6721 cagcaactca accccaccaa ggcggcctcc tttcgcgcat gcctccgaaa gttcgtcacg
     6781 gttgtcgtat tctttcccga tctccacgtc gtgatcggac caccagacct ccaaggagta
     6841 gtggtaaagg gtgccgagcg tgttcttgac cggacgatcg ctcaacacga tgacatgtcg
     6901 ccgtgaggcg acacaggact ccttgatcga gtcgatcgag tcgtgtgaaa aacaatcggc
     6961 gtgtgattgt ccgccgctca tttatcgatc ccccgcgtcc cccgcaggca atgctcgaga
     7021 tttttcagct gtctttggag gcttccgtcg tagaacgccg cctccaaatc ccgcccgagc
     7081 agccgttcag gaccctcggc gtgggcgaag atcacactca caaattgata gacatagcgc
     7141 tcagagtgga tgttatgcag gcgcacgtac tcgatacagt tttcgatcca ggagatcgtg
     7201 gacgactcgg gccgcctctc atactgttcc gggcaatctc gcttcagcgt caaggccaca
     7261 cgggctagaa acgcctgtcg ttgttcggca acaaacgctg ccttttgctc atgcgatatt
     7321 attaacacgt ttttcgagcc ccggcggctg cattgccgaa tccgcattga ttctcgcgcg
     7381 actttatgcg cgttattata cacttgtcag agacagtgta ccagaccttg cgcgacggtg
     7441 cgatctgctg gatggcttga gaagccgtac cgacttcgtc gcgcttcgcg cggactttcg
     7501 atacagcccc tgtaaacgga cccgtgggga catggcacct gggaacatat atcaataaca
     7561 tacatgatct cgccgacttg atgggcggcc atagacggag ggacgaacgc cgtgcccgcg
     7621 tgcgcacatg cg
//
LOCUS       DASWNJ010001774         2186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_343025, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001774 DASWNJ010000000
VERSION     DASWNJ010001774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343025"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VGB85_29670"
     CDS             <1..503
                     /locus_tag="VGB85_29670"
                     /inference="COORDINATES: protein motif:HMM:NF027450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HEY0138304.1"
                     /translation="ADLGALEVAANGELAVAEDAANDPAALAEDAAALDQRHLEQARA
                     RIDEMRAYAECFDCRREQILRHFGEPDTAIVKPCAACDNCDAGHGELLARMEADAPPR
                     PPAEEPAPAPSEPFPVESTVVHAEWGRGRVLRYAEGKIVVDFADVGEKTLGLDFVAAR
                     NLLTPA"
     gene            560..1291
                     /locus_tag="VGB85_29675"
     CDS             560..1291
                     /locus_tag="VGB85_29675"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0138305.1"
                     /translation="MLRRLLVPLLLAACSTKSAPPPAAPAPVLPSAERDFDKDGIVDP
                     RDRCPRDPGDAPLGCPDKDTDGDGLKASVDKCPNFAGPPPDGCPLPDADNDGVANDDD
                     KCQGTFESLNGFQDRDGCPDEVPRDLTRITGVIKGVVFDTNADTLKASSIPVLNRIVD
                     VLKRYPDVRIEISGHIDNTMKVDPASEPSLRRAEVVKQYLVEQGIEAKRLETRGAGAN
                     EPLDSNKTASGRARNRRIEFTILVQ"
     gene            1327..2037
                     /locus_tag="VGB85_29680"
     CDS             1327..2037
                     /locus_tag="VGB85_29680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138306.1"
                     /translation="MHRRILALLLAHLTACTSSSDDTGSASSEGTSGEPDTTTSTSST
                     ADTSTGDPPTSTTGPGDDSGTTSTTSTTGDDSTTGAAPYCNGWQTDEGELYLDLHNRD
                     AMALADGATLPLECGGQGIFMFAMYAEFGGFMPTSDIVDFAVIVDVEGFNNNPEGHFY
                     SATPIGYWVGCDQLDGGPPTGFVPIFPFDNLDDLTALDGKPADIHLVMHTGEGDVTRD
                     FSVVLSVQKDDSWAFCGG"
BASE COUNT          371 a          890 c          643 g          282 t
ORIGIN      
        1 tcgccgacct cggcgcgctc gaggtcgccg ccaacggcga gctcgccgtc gccgaggatg
       61 cagcgaacga ccccgccgcg ctcgccgagg acgccgccgc gctcgaccag cgacacctcg
      121 agcaggcccg ggcccgcatc gacgagatgc gcgcctacgc cgagtgcttc gactgccgcc
      181 gcgagcagat cctgcgccac ttcggcgagc cggacaccgc gatcgtcaag ccctgcgccg
      241 cctgtgacaa ctgtgacgcc ggtcatggcg agctgctcgc ccgcatggaa gccgacgcgc
      301 ccccgcgccc gcccgccgag gagcccgcgc ccgccccctc cgagcccttc ccggtcgaga
      361 gcaccgtcgt ccacgccgag tggggccgcg gccgcgtcct gcgttacgcc gagggcaaga
      421 tcgtcgtcga ttttgcggat gtcggcgaga agaccctcgg cctcgacttc gtcgccgccc
      481 gcaacctgct cacccccgcc taaaaaaccg ccgccgaccc ctcgcgcaca ctggctcgta
      541 ttcgtagtac cctgcggccg tgctccgaag actcctcgtc ccgctcctcc tcgcggcatg
      601 ctccaccaag tctgctccgc ccccggctgc gccggccccg gtgctcccga gcgccgagcg
      661 tgacttcgac aaggacggca tcgtcgaccc gcgcgaccgc tgcccgcgcg accccggcga
      721 tgccccgctc ggctgccccg acaaggacac ggacggcgac ggcctcaagg ccagcgtcga
      781 caagtgcccg aacttcgccg gcccgccgcc cgacggttgc cccttgccgg acgccgacaa
      841 cgacggcgtc gccaacgacg acgacaagtg ccagggcacc ttcgagtccc tcaacggctt
      901 ccaggaccgc gacggctgcc cggacgaggt ccccagggac ctgacccgca tcaccggcgt
      961 gatcaagggc gtcgtgttcg acaccaacgc cgacacgctc aaggccagct cgatccccgt
     1021 gctcaaccgc atcgtcgacg tgctcaagcg ctaccccgac gttcgcatcg agatctccgg
     1081 ccacatcgac aacaccatga aggtcgatcc cgccagcgag ccctcgctgc gccgcgccga
     1141 ggtcgtcaag cagtacctcg tcgaacaggg catcgaggcc aagcgcctcg agacccgcgg
     1201 cgccggcgcg aatgagcccc tcgacagcaa caagaccgcc tccgggcgcg cccgcaaccg
     1261 ccgcatcgag ttcacgatcc tcgtccagtg acgccgaccg cggcgtgaac acgctatcgt
     1321 cggtgcatgc accgacgcat cctcgccctc ctgctcgcgc acctcaccgc ctgcaccagc
     1381 agcagcgatg acaccggctc cgccagcagc gagggcacct ccggtgaacc cgacaccacg
     1441 acgtcgacct ccagcaccgc ggacacgagc accggcgacc cgccgacctc gaccaccggc
     1501 cccggcgacg acagcggcac cacgagcacc acgagcacca ccggcgacga cagcaccact
     1561 ggcgccgccc catactgcaa cggctggcag accgacgagg gcgagctcta cctcgacctc
     1621 cacaaccggg acgcgatggc gctcgccgac ggcgcgacgc tgccgctcga gtgcggcggc
     1681 cagggcatct tcatgttcgc catgtacgcc gagttcggcg gcttcatgcc gaccagcgac
     1741 atcgtcgact tcgccgtgat cgtcgacgtc gagggtttta ataataatcc cgagggccac
     1801 ttctacagcg ccacaccgat cggctactgg gtcggctgcg accagctcga cggcggaccg
     1861 cccaccggct ttgtccccat cttcccgttc gacaacctcg acgacctcac cgcgctcgac
     1921 ggcaagccgg ccgacatcca cctcgtcatg cacaccggcg agggcgacgt cacccgcgac
     1981 ttctccgtgg tgctctcggt gcaaaaggat gacagctggg cgttctgcgg cggctgagct
     2041 accctcgagg gcatgcatag cccttacctg tatatcttgc ccctgctcac cagcgcgctc
     2101 gtgctcgcgg cttgcggacc cgaaaccggc gggtcgaccg acagcaccgg cccggccagc
     2161 ggcgacacct cgaccgccac gaccgg
//
LOCUS       DASWNJ010001775         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_668097, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001775 DASWNJ010000000
VERSION     DASWNJ010001775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668097"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1037)
                     /gene="mtnP"
                     /locus_tag="VGB85_29685"
     CDS             complement(108..1037)
                     /gene="mtnP"
                     /locus_tag="VGB85_29685"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEY0138307.1"
                     /translation="MTHAAPTPPHPASPAARRDVVLGVVGGSGLYEIEGMIGTRLVEV
                     DTPFGRPSDAYTLGELPREGLPPVRAVFLPRHGRGHVLLPTEINYRANVHGFKQLGVT
                     HLLSVSAVGSLREHIAPGHVVVPDQFIDRTTRRIGTFFGEGVVAHAQMGDPVDGLLRR
                     RLVEVARAAGATVHDGGTCMVMEGPAFSTRAESELYRSWGASVIGMTAMPEAKLAREA
                     EIAYALLALSTDYDCWHVSADEVSVADVLAVIHRNVALSRRMISALAASLPLTTQELP
                     YPRALEHAIITAPDRVPAATRAKLDLILGHYLG"
     gene            1120..>2010
                     /locus_tag="VGB85_29690"
     CDS             1120..>2010
                     /locus_tag="VGB85_29690"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY0138308.1"
                     /translation="MRNEVHGCVAAGHPVTARAAAEVLRAGGNAFDAALAAVAAACVA
                     ESVLASPGGGGFLLARPADAPARIYDFFVHTPRVRRPQEEVNAHPVVTDWGTTQQEFQ
                     IGIGTAATPGLVRGLFAIHGDLGRMPMPQVLAPAVAAAHAGVEVTPFQAYLMTLVSPI
                     YLGEPETRALFGNAEGGSLNRGDLLHNPELGALFEQLGREGDGLFYEGEVASAIVEQC
                     RTRGGHITHDDLRNYRVAVREPLTLRYRDATLTTNPPPSAGGTLIAFGLGLLSRVDLA
                     NVTFGSPEHLRVLARVLARTG"
BASE COUNT          286 a          772 c          678 g          274 t
ORIGIN      
        1 atgatccagt cgatggcgac gctaccgacg acgagcaggg aggagtttgc gctgttcatg
       61 aagacaggtc tcttgggaca ggtgcgagaa aagccagcca gtaccagcta gccgaggtaa
      121 tgcccgagga tgaggtccag cttggccctg gtggccgctg gcacgcgatc gggggcggtg
      181 atgatggcat gctcgagcgc ccgcgggtag ggcagctcct gcgtggtcaa cggcaggctc
      241 gcggccagcg cggagatcat gcggcgcgac agggcgacgt tgcggtggat cacggcgagc
      301 acatcggcga ccgagacctc gtcggcgctg acgtgccagc agtcgtagtc ggtgctcagc
      361 gcgagcagtg cgtaggcgat ctcggcctcg cgggcgagct tcgcctcggg catcgccgtc
      421 atgccgatca cgctcgcgcc ccagctgcgg tacagctccg actcggcgcg cgtgctgaag
      481 gccgggccct ccatcaccat gcacgtgccg ccgtcgtgga ccgtcgcccc cgcggcccgc
      541 gcgacctcga cgagccgccg gcgcagcagg ccgtcgaccg ggtcgcccat ctgcgcgtgt
      601 gctaccacac cctcgccaaa gaaggtgccg atccgccgcg tcgtgcgatc gatgaactgg
      661 tcgggcacga ccacgtgccc tggcgcgatg tgctcgcgca aggaccccac cgccgacacc
      721 gacagcaagt gcgtcacgcc gagctgcttg aagccgtgca cgttggcccg atagttgatc
      781 tccgtcggca gcaacacgtg tccgcgtccg tgtcgcggca ggaacaccgc gcgcaccggc
      841 ggcaggccct cgcgcggcag ctcgccgagc gtgtacgcgt ccgacgggcg accgaagggc
      901 gtgtcgacct cgacgagccg cgtcccgatc atcccttcga tctcgtacag ccccgagcca
      961 ccgaccacgc cgaggaccac gtcacgacgg gccgcggggg acgcaggatg cggaggggtc
     1021 ggagctgcgt gggtcatgcg ggcggaggat agcggcgccc ccgcgccccg ccaagccttg
     1081 gcgcgcccgc gctccccgcg ttataagtcg cgggcccgca tgcgaaacga agtccacggc
     1141 tgcgtcgccg caggtcaccc cgtcaccgcc cgcgccgccg ccgaggtgct gcgcgccggt
     1201 ggcaacgcct tcgacgccgc gctcgcggcc gtcgccgccg cctgcgtcgc ggagtccgtg
     1261 ctcgcctccc cgggcggcgg cggcttcctg ctcgcccgcc ccgcggacgc gccggcccgc
     1321 atctacgact tcttcgtgca cacgccgcgc gtgcgccggc cccaggaaga ggtcaacgcc
     1381 cacccggtcg tcaccgactg gggcaccacc cagcaagagt tccagatcgg tatcggcacc
     1441 gccgcgaccc cgggcctcgt gcgcggcctg tttgcgatcc acggcgacct cggccgcatg
     1501 ccgatgccgc aggtcctcgc gcccgcggtc gcggcggcgc acgccggtgt cgaggtcacg
     1561 cccttccagg cctacctgat gaccctcgtc tcgccgatct acctcggcga gcccgagacg
     1621 cgcgcgctgt tcggcaacgc cgagggcggc tcgctcaacc gcggcgacct cctgcacaac
     1681 cccgagctcg gcgcgctgtt cgagcagctc ggccgcgagg gcgacggcct gttctacgag
     1741 ggcgaggtcg cctccgcgat cgtcgagcag tgccggaccc gcggcggtca catcacccac
     1801 gacgaccttc gcaactaccg cgtcgccgtg cgcgagccgc tgaccctgcg ctaccgcgac
     1861 gccaccctga cgaccaaccc gccgccctcc gccggcggca cgctgatcgc cttcggcctg
     1921 ggtctgctgt cccgcgtcga cctcgccaac gtcaccttcg gatcacccga gcacctgcgc
     1981 gtgctcgccc gcgtgctcgc ccgcaccggc
//
LOCUS       DASWNJ010001776         5864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_67051, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001776 DASWNJ010000000
VERSION     DASWNJ010001776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5864
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67051"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..4078
                     /locus_tag="VGB85_29695"
     CDS             71..4078
                     /locus_tag="VGB85_29695"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin"
                     /protein_id="HEY0138309.1"
                     /translation="MPRAPGNHSTDLGPPRPRLFLKNSPWRLAAALAVLATPGMSACV
                     SDPDCGICDPDNLILQSIAGVNYAGKTVKLLGPECVGDACPGPMQEGQYFVEKIIPCI
                     ETADALDSPRGVDEWCKVSPMVVDSGLQFIFNNLLDPTSVELVRKKADNPQLFEVFDW
                     KTQIVHLEGPITRFNGDYRPGATPAAPDTVERAQSLACIDNLRRLGRPYDHTVLASDP
                     GICDGTHVGPDGRVWPLRTQLQREDAGGRTAPTTVETWRGETDTRAATQSCTRPQDGV
                     DTCCDVCDYELAVNVSKYGVLAPVDELAPAGDRRRRFADAITCDPGGDKLRECRDFTP
                     HVDRSHEVRSYEYTWDGARQRFRVPRPDKIRETHPDDRPADAEQRTVPCQTDEDCTGS
                     SQADLPGMECVGHLADDPGTGCRVGDDCVERRCVAEWFVDCRPDKDTTGAQGYCVDRR
                     WSGEGVAACFTNGAPYYVCEDTETCAAEDRTDPGRRQSEGARMSLADADRDGRVEAIE
                     GCRASLGGPDGGPCDPLDQPGVLPIVRHDRLDNLPTPTRTCICEDEPAEGCADFVASL
                     CHEDGDPKKPIAAEKRGQYAVKFVTRFGGVIYDPALKGVQFLPADLGGIPRSLVETCS
                     RTRSKGAGPLNLKDGWRANDAGPELFEDADRAMCSSSEYRVVFNHEPAPGQSPLEYIR
                     DKVGNTLRGKSTYVLHTPDFHVVPGSGFPTDNLRIGACDDFELRFSNKYDLDPRNLRK
                     LQLVEISADGAQQLALVAGGPDCSDDPDAAVPCLTIDVRDQDIGSLRVRIDAQRFGAD
                     HLVPGHRYRLAVPGLELAPGETVFDVIAAGGERYLAAFQDACGMPLVTSMPRLDAAGK
                     LDPDGRPRDPDSHYDFAIDPPKPKEDREQDGVQFSCDNAPDNYNPDQEDMDQDGWGDI
                     IDLCATIPEANNSADTDKDGVGNSCDRCPRALTAYNRQFDETTAKLYMRVRNIPHQQD
                     ADQDGVGDACDNCVVRANCGDFGPEAEGLRPAHIGAPVPHDKDGVCQSDVDGFAFVGD
                     ACVVDGEPLQLPGAAARVGVADDEDFDQDGIANLSDRCPRQPVTRIECTLPEDCPANA
                     ECTGGLCNHVDTDNDAHGDICDTCPSAQNPGQTLDGAMQQDDPDADFVGSKCETNTAC
                     DARPDPRPIAFYSETSAGQCCVQLFSELAGRLDPGLARRDLDAQTCELVDPAVPILLD
                     CPKDQEEITCRVLPQRARERPGVVDLPPGCDGPGEPYTLDHPDIHGDVDLLYQHMCLM
                     PQPDQDFDGIGDACDLCPFAFDPENAKYKDANGKLWPTSGKYCAGEYDPENVLTSCEE
                     AVDSDGEGSSGDSTG"
     gene            4150..4473
                     /locus_tag="VGB85_29700"
     CDS             4150..4473
                     /locus_tag="VGB85_29700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138310.1"
                     /translation="MTTQTIDIVHNPANPGSEQLLDLPPGGTIVLYGEHADAPLHVRI
                     TSLKREGEAEGQFLLEGFIKLPGETTEARVASSFYNARTGRGTLHVEAEFADRYQALA
                     GREPG"
     gene            4525..5316
                     /locus_tag="VGB85_29705"
     CDS             4525..5316
                     /locus_tag="VGB85_29705"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0138311.1"
                     /translation="MFRMKHRWRALPWVLLCACKPSAPEITVPPVVEAVAPAPVGEIP
                     DRDGDGALDASDRCPDEPEDLDGYEDDDGCADRDNDGDKILDAHAFVAGRWTNCDYGL
                     INGVAQDCRNMPEDPDGVADSDGCPEYDFDHCQLQMDSIPLDRRGRPSADAPRILDEL
                     AATLHAAPGIAIRVAAHLDRRDGLEAAKTVTRRVAEGVIDELVRRGIVRERLEAIGLG
                     SEMPVADNRTAAGRARNRRVEFTLQSCADYRTRRPPGPDLPQVCR"
     gene            complement(5332..5817)
                     /locus_tag="VGB85_29710"
     CDS             complement(5332..5817)
                     /locus_tag="VGB85_29710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138312.1"
                     /translation="MPDSPALDAAPRRGVVQFTRATGLALPVVAFVVAETLAAFAGII
                     RDYLAIHYDLWFELGMVIGQVLFQWAVLWRRTWTERLDYAVILVFVSGLGAALLWPLL
                     LWDRAAPVEPLAAVAYFFAVVAVMFVVHLRLVRQAGLPLVLCMTWVIYRLLILAVVLP
                     R"
BASE COUNT          994 a         2229 c         1920 g          721 t
ORIGIN      
        1 cgcgcgcgag ttccgatcgc ggtccctggc gagaccacgc cgtgcaagtg acagtgcacg
       61 ggaggtcctc atgccacgcg ctccaggcaa ccactccacc gatctcggcc ccccgcgacc
      121 ccgcctcttt ttaaaaaaca gcccctggcg gctcgccgcg gcgctcgccg tgctcgccac
      181 gcccggcatg tccgcgtgcg tcagcgaccc ggactgtggg atctgcgatc ccgacaatct
      241 gatcttgcaa agcatcgccg gggtcaacta cgccggcaag accgtcaagc tgctcgggcc
      301 cgagtgcgtc ggagacgcgt gtccaggtcc gatgcaggag gggcaatact tcgtcgagaa
      361 gatcattcca tgcatcgaga ccgccgacgc cctcgattcc ccgcgcggcg tcgacgagtg
      421 gtgcaaggtc tcgccgatgg tcgtcgactc cggcctgcaa ttcattttca ataatttgct
      481 cgacccgacc tcggtcgagc tggtgcgcaa gaaggccgat aatccccagc tgttcgaggt
      541 cttcgactgg aagacgcaga tcgtccacct cgagggcccg atcacccgct tcaacggcga
      601 ctaccgaccc ggcgcgaccc cggccgcccc cgacaccgtc gagcgcgcgc agagcctcgc
      661 gtgcatcgac aacctgcgcc gcctcggtcg cccctacgat cataccgtgc tcgccagcga
      721 ccccggcatc tgcgacggca cgcacgtcgg cccggacggc cgcgtgtggc cgctgcgcac
      781 ccagctgcag cgcgaggacg ccggggggcg caccgccccg accaccgtcg agacctggcg
      841 cggcgagacc gacacgcgcg ccgccaccca gtcgtgcacg cgcccgcagg acggcgtcga
      901 cacctgctgc gacgtctgcg actacgagct cgccgtcaac gtatccaagt atggcgtgct
      961 cgcgccggtc gacgagctcg cgccggccgg cgaccgccgc cgccgcttcg cggacgcgat
     1021 cacctgtgac ccgggcggcg acaagctgcg tgaatgtcga gacttcacgc cgcacgtcga
     1081 ccgcagccac gaggtccgca gctacgagta tacgtgggac ggcgcgcgcc agcgcttccg
     1141 tgtcccgcgg cccgacaaga tccgcgagac ccaccccgac gaccggccgg cggacgccga
     1201 gcagcgcacc gtgccctgcc agaccgacga ggactgcacc ggcagctccc aggccgacct
     1261 cccgggcatg gagtgcgtcg gccacctcgc cgacgacccc ggcaccggct gccgcgtcgg
     1321 cgacgactgc gtcgagcgcc gctgcgtcgc cgagtggttc gtcgactgcc gccccgacaa
     1381 ggacaccacc ggcgcgcagg gctactgcgt cgaccggcgc tggagcggcg agggtgtcgc
     1441 ggcctgcttc accaacggcg cgccttacta cgtgtgcgag gacaccgaga cctgcgccgc
     1501 ggaggaccgg accgacccgg gccggcgcca gagcgagggc gctcgcatgt cgctcgcgga
     1561 cgccgaccgc gacggccgcg tcgaggcgat cgagggctgc cgcgcgagcc tcggcggccc
     1621 ggacggtggc ccctgcgacc cgctcgacca gcccggcgtg ctgccgatcg tccgtcacga
     1681 ccgcctcgac aacctgccga cgccgacccg cacctgcatc tgcgaggacg agcccgccga
     1741 gggctgcgcc gacttcgtcg cgtcgctgtg ccacgaggac ggcgacccca agaagccgat
     1801 cgcggccgag aagcgcgggc aatatgccgt caagttcgtg acgcgcttcg gcggcgtcat
     1861 ctatgacccc gcgctcaagg gcgtgcagtt cctcccggcc gacctcggcg ggatcccgcg
     1921 cagcctggtc gagacctgct cgcgcacgcg cagcaagggc gccggcccgc tcaacctcaa
     1981 ggacggctgg cgcgccaacg atgccggccc cgagctgttc gaggacgccg accgggcgat
     2041 gtgctcgagc agcgagtatc gcgtcgtctt caatcatgag cccgcgccgg gccagagccc
     2101 gctcgagtac atccgcgaca aggtcggcaa tacactgcgc ggcaagtcga cctacgtgct
     2161 gcacacgccc gacttccacg tcgtgcccgg cagcggcttc ccgaccgaca acctgcggat
     2221 cggcgcgtgc gacgacttcg agctgcgctt ctccaacaag tacgacctcg acccccgcaa
     2281 cctgcgaaag ctccagctcg tcgagatctc ggcggacggc gcccagcagc tcgcgctcgt
     2341 cgccggcgga cccgactgct cggacgaccc ggacgccgcg gtgccgtgcc tgacgatcga
     2401 cgtgcgcgac caggacatcg gatccctgcg cgtgcgcatc gacgcccagc gcttcggcgc
     2461 cgaccacctc gtgcccgggc accgctatcg cctcgcggtc cccggcctcg agctcgcgcc
     2521 cggcgagacc gtgttcgacg tgatcgccgc cggcggcgag cgctacctcg ccgcgttcca
     2581 ggacgcctgc ggcatgccgc tggtgacctc gatgccgcgc ctcgacgccg ccggcaagct
     2641 cgaccccgac ggccgcccgc gtgaccccga cagtcactac gactttgcga tcgacccgcc
     2701 caagcccaag gaggaccgcg agcaggacgg cgtgcagttc tcctgcgaca acgcgcccga
     2761 caactacaac cccgaccagg aggacatgga ccaggacggc tggggcgaca tcatcgacct
     2821 gtgcgcgacg atccccgagg ccaacaacag cgccgacacc gacaaggatg gcgtcggcaa
     2881 cagctgcgac cgctgcccgc gggccctgac cgcctacaac aggcagttcg acgagaccac
     2941 cgccaagctg tacatgcgcg tccgcaacat cccgcaccag caggacgccg accaggacgg
     3001 cgtcggggac gcgtgcgaca actgcgtcgt gcgggccaac tgcggcgact tcgggcccga
     3061 ggccgagggc ctgcgccctg cccacatcgg cgcccccgta ccgcacgaca aggacggcgt
     3121 gtgccagtcc gacgtcgacg gcttcgcctt cgtcggcgac gcctgcgtcg tcgacggcga
     3181 gccgctgcag ctgccgggcg cagccgcccg ggtcggcgtc gccgacgacg aggacttcga
     3241 tcaggacggc atcgccaacc tgagcgaccg gtgcccgcgc cagccggtca cgcggatcga
     3301 gtgcaccctg ccggaggact gcccggcgaa cgccgagtgc accggcggcc tgtgcaacca
     3361 cgtcgacacc gacaacgacg cgcacggcga catctgcgac acctgcccgt cggcgcagaa
     3421 ccccggccag acgctcgacg gcgcgatgca gcaggacgac cccgacgccg acttcgtcgg
     3481 cagcaagtgc gagaccaaca ccgcgtgcga cgcccgcccc gacccgcgcc cgatcgcgtt
     3541 ctacagcgag accagcgcgg gccagtgctg cgtgcagctg ttctcggagc tggcaggtcg
     3601 cctcgacccc ggcctggccc gccgcgacct cgacgcgcag acctgcgagc tcgtcgaccc
     3661 cgcggtcccg atcctcctcg actgtcccaa agaccaggaa gagatcacct gccgcgtcct
     3721 gccccagcgc gcgcgcgagc gccccggcgt cgtcgacctg ccgcccggct gcgacggccc
     3781 cggcgagccc tacaccctcg accaccccga catccacggc gacgtcgacc tgctgtatca
     3841 gcacatgtgc ctgatgccgc agcccgatca ggacttcgac ggcatcggcg acgcatgcga
     3901 cctgtgcccc ttcgccttcg accccgagaa cgccaaatac aaggacgcca acggcaagct
     3961 gtggccgacc tcgggcaagt actgcgccgg cgagtacgac cccgagaacg tgctcacctc
     4021 ctgcgaggag gccgtcgact ccgatggtga ggggtcgagc ggcgacagca ccggctgagc
     4081 gccagcgcgc ggcgcgatca ctcgcgtcgt ggcgcccgtg acgaccgact aagatcgcgg
     4141 cccgcgacca tgaccacgca aacgatcgac atcgtccaca accccgcgaa ccccggcagc
     4201 gagcagctgc tcgacctccc gcccggcggc acgatcgtcc tgtatggcga gcatgcggac
     4261 gccccgctgc acgtgcggat caccagcctc aagcgcgagg gcgaggccga gggccagttc
     4321 ctgctcgagg gttttattaa attgcccggc gagaccaccg aggcccgcgt ggccagcagc
     4381 ttttataacg cgcggaccgg ccgcggcacg ctccacgtcg aggccgagtt cgccgaccgc
     4441 taccaggcgc tcgcggggcg cgagcccggc tgacagtcag cgcggtgcgt gcgaatatcg
     4501 gtgcgcgtgt cgcatcgttc acgcatgttc cgaatgaagc accgctggcg cgcgctgccg
     4561 tgggttctgc tgtgcgcgtg taagccgagc gcgccggaga tcaccgtgcc gcccgtggtc
     4621 gaggcggtgg ctccggctcc cgtcggcgaa atcccggaca gggacggcga cggtgccctc
     4681 gacgcgagcg accgctgccc agatgagccc gaggatctcg atggatacga ggacgacgac
     4741 ggctgcgccg atcgcgacaa cgacggcgac aagatcctcg acgcgcacgc cttcgtcgcc
     4801 ggccgctgga ccaactgcga ttatgggctc atcaacggcg tcgcgcagga ctgccgcaac
     4861 atgcccgagg accccgacgg ggtcgccgat agcgacggct gtccagagta cgatttcgat
     4921 cattgccagc tccaaatgga cagcatcccc ctcgatcgtc gcgggcgacc ctccgccgac
     4981 gcgccgagga tcctcgacga gctcgccgcg acgctgcacg ccgcgccggg gatcgccatc
     5041 cgcgtcgctg cgcatctcga ccggcgggat ggcctcgagg ccgcaaagac cgtcacgcga
     5101 cgcgtggccg agggggtgat cgacgagctc gtccgtcgcg gcatcgtccg cgagcgcctc
     5161 gaagcgatcg gcctgggtag tgaaatgccg gtcgccgaca accgcaccgc cgccggtcgc
     5221 gccaggaatc gccgcgtgga gttcacgctc cagtcctgcg ccgactaccg aacccgccgg
     5281 cccccggggc ctgacctgcc gcaagtgtgt cgctgacggg gagcgatcgc atcagcgcgg
     5341 cagcacgacg gcgaggatca gcaatcgata gatcacccac gtcatgcaca ggaccagcgg
     5401 cagcccggcc tgccgcacga gccgcaggtg cacgacgaac atcaccgcga cgaccgcgaa
     5461 gaagtaagcg acggccgcga gcggctcgac gggcgcggcg cggtcccaca gcagcagcgg
     5521 ccacagcagc gccgcgccga ggccggagac gaacacgagg atcacggcgt agtcgagccg
     5581 ctcggtccac gtgcgccgcc acagcaccgc ccactggaac agcacctggc cgatcaccat
     5641 gccgagctcg aaccacaggt catagtgaat ggcgaggtag tcgcggatga tccccgcgaa
     5701 ggcggcgagc gtctcggcga cgacgaacgc gacgaccggc agcgcgagcc cggtcgcccg
     5761 agtgaactgg acgacgccgc ggcgcggcgc ggcgtcgagg gcgggtgagt cgggcacgcg
     5821 cgcgatggta cccgtcgatc gatccggcga ccaccgcgga ccaa
//
LOCUS       DASWNJ010001777         3456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_491059, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001777 DASWNJ010000000
VERSION     DASWNJ010001777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491059"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..3093)
                     /locus_tag="VGB85_29715"
     CDS             complement(121..3093)
                     /locus_tag="VGB85_29715"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEY0138313.1"
                     /translation="MPGLALGLVLTGLVCLPSPAADPALRPMDGPLAAPDSVATRDLP
                     RPPLPAIRTFADDPLKVQQVVLANGLTVLLSVNRERPEVFGAVVVRTGGRNDPPGDTG
                     MAHYLEHMLFKGTQDLGTTDWDAERPLQEKLEALYERKRQTPKSGHAAIDAEITRTVK
                     QTYAYAVPNEIDQLLAQLGGTGVNAFTTYDETVYHNTFPASQIDAWLAIYAHRFQDPV
                     FRLFPTELESVYEEKNTAIDTTGYELFRTFMRGAFPGHPYGANDILGEVEHLKRPSLR
                     AMKAYFERYYVPQNMALVLSGDLDVERALPMIEARFGQWKPGPDPKPPVLQVQPFAED
                     QRLRARATPVRVGAIAYRTVPESHPDYPALLLARRLLSNAQRSGLIDRLSDDGALLFA
                     VHVPADLVDTNLDVVAYIPRILTQSFRGAERKVLAQFAKIRDGEFDESTFAALKEGLI
                     AAETARWESNQARTLAIAHAFVARQGWQGQIRYLERLRTVTRADVQRVAQQLFAERRL
                     VLRSRMGFPKKTRLAKPTYPPVTPNGGHSKFFNAMRATPGPPPRVDLVDMEGATVVVP
                     VRDGVTLRANTNPFNDLYQLELRFGVGTDKLRELDILAQYLERTGTLQHPPEQFRRRL
                     FELSTTLTARPHLDRFVVKLAGPQRHMHEALVLLAELMQSPAAERRPLRQIRRETWAL
                     RRYARQDPRSVAEALRDHVLYGDNSPYHREHGPRGARRLRARALLDAWREVQSHALEI
                     GYVGREAPAKVAAAVGERLRFAAQLQPAQPPVVYPRQTPAQTTIYFVPDRSAVQTQLW
                     FAVEGDPVPPAELAAAEAFGEYLGGGMAGLVFQEVREFRALAYSASASFPRDESPAQR
                     AQLLGHVGCQADKTFDVLDVMLGLITHMPERPDRLGLVRSALIRGQETESPSFRALQD
                     KLREWRHRGYHEDPRRALLPAYADLDFDDIITFYKRHVAGRPLAIMIVGDPRKADPAK
                     LRKYGRVVRLRERKLFSP"
     gene            3208..>3456
                     /locus_tag="VGB85_29720"
     CDS             3208..>3456
                     /locus_tag="VGB85_29720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138314.1"
                     /translation="MDLLGTFLLPAHEIAAAIGAAGQRLFYLSPDSVAPHPNPRMLGA
                     YTRVVLIPRLDELPDLQAAQRRWCAGAALELGEAVPRID"
BASE COUNT          457 a         1102 c         1356 g          541 t
ORIGIN      
        1 ctgctggtgc gacatcaatt gcttcatgaa cggagactgc tgcctgcagg atttctgcgt
       61 gtagtggctt tgtctcggcg gcccaccctc gttcatgcgg ggtgacgcct gatcacgagg
      121 ctaggggcta aacagcttgc gctcgcgcag gcggacgacc cggccgtatt tgcgcagctt
      181 cgccggatcc gccttgcggg ggtcgccgac gatcatgatc gccagcgggc gtccggcgac
      241 gtggcgttta taaaaagtga tgatgtcgtc gaagtcgagg tcggcgtagg ccgggagcag
      301 ggcgcggcgg gggtcctcgt ggtagccgcg gtgacgccac tcgcggagct tgtcctgcag
      361 cgcgcgaaac gacgggctct cggtctcctg gccgcggatc agcgcggagc ggaccagccc
      421 gaggcggtcg gggcgctcgg gcatgtgcgt gatcaggccg agcatgacgt cgaggacgtc
      481 gaaggtcttg tcggcctggc agccgacgtg gccgagcagc tgggcgcgct gggcgggcga
      541 ctcgtcgcgc gggaagctgg cgctggcgga gtaggcgagg gcccggaact cgcggacctc
      601 ctggaacacg aggccggcca tgccgccgcc gaggtactcg ccgaaggcct cggcggcggc
      661 gagctcggcg ggtgggaccg gatcgccctc gaccgcgaac cacagctgcg tctgtacggc
      721 gctgcgatcc ggcacgaagt agatcgtcgt ctgcgcgggt gtctgtcgcg ggtacacgac
      781 cggcggctgc gcgggctgca gctgggccgc gaaccgcagg cgctcgccga cggcggcggc
      841 gaccttcgcg ggcgcctcgc ggccgacgta gccgatctcg agcgcgtggc tctggacctc
      901 gcgccacgcg tcgagcaacg cgcgcgcgcg caggcgtcgg gcgccgcgcg ggccgtgttc
      961 gcggtggtac ggggagttgt caccgtagag cacgtggtca cgcagggcct cggcgacgct
     1021 gcgcgggtcc tggcgggcgt agcggcgcag ggcccaggtc tcgcgccgga tctgccgcag
     1081 cggccggcgc tcggccgcgg gtgactgcat cagctcggcg agcaacacga gcgcctcgtg
     1141 catgtgccgc tgcgggccgg cgagcttgac gacgaagcgg tcgaggtggg gcctggccgt
     1201 cagggtggtg ctgagctcga acaggcgccg gcggaattgt tcgggcgggt gctgcagcgt
     1261 gccggtgcgc tcgaggtact gcgcgaggat gtcgagctcg cggagcttgt cggtgccgac
     1321 gccgaagcgc agctcgagct ggtagaggtc gttgaagggg ttggtgttgg cgcgcagcgt
     1381 cacgccgtcg cggacgggca cgaccacggt ggcgccctcc atgtcgacga ggtcgacccg
     1441 cggcgggggt ccgggcgtcg cgcgcatcgc gttgaagaac ttcgagtggc cgccgttggg
     1501 cgtcactggc gggtaggtcg gcttggcgag gcgggtcttc tttggaaaac ccatgcgcga
     1561 tcgcaggacc aggcggcgct cggcgaacag ctgttgtgcc acgcgctgga cgtccgcgcg
     1621 ggtgactgtg cgcaggcgct cgaggtagcg gatctggccc tgccaaccct gacgcgcgac
     1681 gaaggcgtgg gcgatcgcca gggtgcgggc ctggttgctc tcccagcggg cggtctcggc
     1741 ggcgatcagg ccctccttga gcgcggcgaa ggtgctctcg tcgaactcgc cgtcgcggat
     1801 cttggcgaac tgcgcgagca ccttgcgctc ggcgccgcga aagctctgcg tcaagatccg
     1861 cgggatgtag gcgacgacgt cgaggttggt gtcgacgagg tcggccggca cgtgcacggc
     1921 gaacagcagg gcgccgtcgt cggacaggcg gtcgatcagg cccgagcgct gggcgttgga
     1981 cagcaggcgg cgggcgagca gcagcgccgg gtaatccggg tgcgactcgg gcacggtgcg
     2041 gtaggcgatc gcgccgacgc gcaccggggt ggcgcgcgcg cgcaggcgct ggtcctcggc
     2101 gaacggctgg acctgcaaca ccggcggctt cgggtccggt ccgggcttcc actggccgaa
     2161 cctcgcctcg atcattggta gcgcgcgctc gacgtcgagg tcgcccgaga gcaccagggc
     2221 catgttctgc ggcacgtagt agcgctcgaa gtaggccttc atcgcgcgca gcgacgggcg
     2281 cttgaggtgc tcgacctcgc cgaggatgtc gttggcgccg taggggtgcc cggggaaggc
     2341 gccgcgcatg aaggtccgga acagctcgta gccggtggtg tcgatcgcgg tgttcttctc
     2401 ctcgtagacc gactcgagct cggtcgggaa caggcgaaac accgggtcct ggaagcggtg
     2461 ggcgtagatc gccagccagg cgtcgatctg actcgcgggg aaggtgttgt gatagacggt
     2521 ctcgtcgtag gtggtgaacg cgttgacgcc ggtgccgccg agctgggcca gcagctggtc
     2581 gatctcgttg ggcaccgcgt aggcgtaggt ctgcttgacg gtgcgggtga tctccgcgtc
     2641 gatcgccgcg tgtccggact tcggggtctg gcgcttgcgc tcgtacagcg cctcgagctt
     2701 ctcctgcaac gggcgctcgg cgtcccagtc ggtcgtgccg agatcttgcg tgcccttgaa
     2761 cagcatgtgc tcgaggtagt gggccatgcc ggtgtcgccg ggcgggtcgt tcctcccgcc
     2821 ggtgcgcacg acgaccgcgc cgaacacctc cgggcgctcg cgattgacgc tcaggagcac
     2881 cgtcaggccg ttggcgagca cgacctgctg gaccttgagc gggtcgtccg cgaaggtccg
     2941 gatcgccggc agcgggggcc ggggcaggtc gcgggtggcg accgagtccg gggccgccag
     3001 ggggccgtcc atcggccgca gcgccggatc ggccgccggg gacggcaagc agacgaggcc
     3061 ggtcaggacg aggccgagag cgaggccggg cacctctcct tgctatcacg gggcgccgac
     3121 ccggcgggtc ggcgatcttc gatgtcgtcg tcgtgctcgc tggcgttgtg cgcgcccggc
     3181 gaagtgctaa aacgcgggcc gccccgcatg gacctcctcg gcaccttttt gctccccgcc
     3241 cacgagatcg cggccgcgat cggcgctgcc gggcagaggc tgttctatct ctcgcccgac
     3301 agcgtcgcgc cgcacccgaa cccgcgcatg ctcggcgcgt acacgcgggt ggtgctgatc
     3361 ccccggctcg acgagctgcc cgatctccag gcggcgcagc gtcgctggtg cgccggcgcg
     3421 gcgctcgagc tcggggaggc ggtgccgcgg atcgac
//
LOCUS       DASWNJ010001778         1834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_103033, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001778 DASWNJ010000000
VERSION     DASWNJ010001778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103033"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="VGB85_29725"
     CDS             complement(<1..1040)
                     /locus_tag="VGB85_29725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEY0138315.1"
                     /translation="MSVTLEQVKAALAPIKDPATGRELVADGRAEARVGADEVALKID
                     LISPGFPLRGELDAALRAAIEPMCGAAGIPLRIEYGLRVPSKPARQDLNRLPTVKNII
                     AVAAGKGGVGKSTVSVNIAMALKQLGARVAVLDADIYGPSVPKMLGAPSRETSTTAEG
                     DRISPAIFRGIPVMSVAFFVEPGSAVIWRGPMIHKLLKQFLEDVNWGEIDYLIVDLPP
                     GTGDAQLSLAQLIPITGAVIVTTPQEVALIDVRRAVNMFNKLEVPILGVVENMSHYTC
                     PACGHHDHIFASGGGVTLARELGVELLGQVPIDNRVSYGGESGVPVVEGAPGSEQARV
                     LTEVAARAAFAV"
     gene            complement(1037..1420)
                     /locus_tag="VGB85_29730"
     CDS             complement(1037..1420)
                     /locus_tag="VGB85_29730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEY0138316.1"
                     /translation="MKQPTSLVFVCQNLRDADNPKGSCMARGSKAVLERMKGEREALG
                     LKHSLRVMGATCLGSCESGVTVLVVDEREGATFYGRMTPALGEALVREHVAGGAAGPE
                     LQRHRLPKSNLLDVSAIESRDEAKP"
     gene            1505..>1834
                     /locus_tag="VGB85_29735"
     CDS             1505..>1834
                     /locus_tag="VGB85_29735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY0138317.1"
                     /translation="MAVLDIVTYPDPRLREPTFDVQDINADVRQLVRDLIDTMYSLNA
                     AGIAAIQVGRLERIFIIDGKVADPEAGDDAKPLVLINPELVETGRGLAVAEEGCLSFP
                     NVFVDIKR"
BASE COUNT          268 a          669 c          614 g          283 t
ORIGIN      
        1 ctgaccgcga acgcggccct ggcagcgacc tcggtcaata cgcgggcctg ctcgctgccg
       61 ggcgcgccct cgacgaccgg cacgcccgac tcgccgccgt agctgacgcg attgtcgatc
      121 gggacctggc cgagcagctc gacgccgagc tccctggcca gcgtcacacc accgccgctg
      181 gcgaagatgt gatcgtggtg gccgcaggcc gggcaggtgt agtggctcat gttctcgacg
      241 acgccgagga tcgggacctc gagcttgttg aacatgttga ccgcgcggcg cacgtcgatc
      301 agcgcgacct cctgcggggt cgtgacgatc acggcgccgg tgatcgggat cagctgcgcg
      361 agcgagagct gggcgtcacc ggtgccgggc ggcaggtcga cgatcaggta gtcgatctcc
      421 ccccagttca catcttccaa aaattgcttg agcagcttgt ggatcatcgg cccgcgccag
      481 atgaccgcgc tgcccggctc gacgaagaac gcgacgctca tcaccgggat cccgcgaaag
      541 atcgccgggc tgatgcggtc gccctcggcc gtggtcgagg tctcccgcga cggcgcgccg
      601 agcatcttcg gcacgctcgg gccgtagatg tcggcgtcga gcaccgccac gcgcgcgccc
      661 agctgcttga gcgccatcgc gatgttgacc gagaccgtgc tcttgccgac cccgcccttg
      721 cccgccgcga ccgcgatgat gttcttcacc gtcggcaggc ggttgaggtc ctggcgggcc
      781 ggcttgctcg ggacccgcag gccgtactcg atgcgcagcg gtatgcccgc cgcgccgcac
      841 atcggctcga tcgccgcgcg cagcgccgca tcgagctcgc cacgcagcgg gaagccgggc
      901 gagatcaggt cgatcttgag cgcgacctcg tcggcgccga cacgcgcctc ggcgcgcccg
      961 tcggccacca gctcgcggcc ggtcgccggg tccttgatcg gcgccagcgc ggccttcacc
     1021 tgctcgagtg ttacgctcat ggcttggcct cgtcacggga ctcgatcgcc gagacgtcga
     1081 gcaggttgga cttcggcagg cgatgccgct gcagctccgg cccggcggcc ccgccggcga
     1141 cgtgctcgcg gaccagggcc tcgccgagcg ccggggtcat gcggccgtaa aaggtcgcgc
     1201 cctcgcgctc gtcgacgacc agcaccgtca cgcccgactc gcacgagccc aggcaggtgg
     1261 cgcccatgac gcgcagcgag tgcttgaggc ccagggcctc gcgctcgccc ttcatgcgct
     1321 cgagcacggc cttgctcccg cgcgccatgc agctgccctt cgggttgtcc gcgtcacgca
     1381 ggttctggca gacgaacacg agggaggtcg gctgcttcat gtgcgctggg gtacatacca
     1441 cgggggcttt gcgcgaccat cggcgggctt tatagtcggg gcggagcaac cgcacaccct
     1501 cgccatggcc gtcctcgaca ttgtcacgta ccccgacccc cgcctgcgcg agccgacctt
     1561 cgacgtgcag gacatcaacg ccgatgtccg gcagctcgtg cgcgacctga tcgacacgat
     1621 gtactcgctc aacgcggcgg gcatcgctgc gatccaggtc ggtcgactcg agcgcatctt
     1681 catcatcgac ggcaaggtcg cggaccccga ggccggcgac gacgccaagc cgctcgtgct
     1741 catcaacccc gagctcgtcg agaccggccg cggcctcgcc gtcgccgagg agggctgcct
     1801 cagcttcccc aacgtgttcg tcgacatcaa gcgg
//
LOCUS       DASWNJ010001779         1518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_383322, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001779 DASWNJ010000000
VERSION     DASWNJ010001779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383322"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VGB85_29740"
     CDS             <1..759
                     /locus_tag="VGB85_29740"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0138318.1"
                     /translation="GRQEEYDLVVIAEGVGSSTRELVFAGENRPRWIDLTMGYFTIPK
                     AAGDSEVARWFTAGGGRSVLLRPDKRGTTRALLNFQAPPQGEDRLELAAQKALLRRRF
                     ADAGWETPRVLDGLDSSTDFYMDVLRQVKLARWSKGRVVLTGDAAWCATPLSGIGTTL
                     AIVGPYVLAGELCKQADIAGALAEYERIMRPYVHKGQDFPKFMARMAQPRSRLGVRLQ
                     GALLGVAAAPGLRKLVAKLFSPAADDVELQDYRI"
     gene            complement(882..>1518)
                     /locus_tag="VGB85_29745"
     CDS             complement(882..>1518)
                     /locus_tag="VGB85_29745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138319.1"
                     /translation="IGGTVEPSSTGDTTMMAAGTSTGDATTTSGDASSGGFKLDLPVP
                     ESMPIPVHDIPGLTSITFYEATSGVMEYTFAVDGSELNQQLPDPLSNANRDIEGTSVE
                     FYDVYYSEEDGSFNPDGSYLTIAGSFGAKLPAGGGLNLAEISLNYADDSVEFGSFLAS
                     FQGLGDNYVPGSEPNAIDGDLMTHTTMGNNVDEPGKRLRVTLGFNSSLMPG"
BASE COUNT          244 a          506 c          517 g          251 t
ORIGIN      
        1 ggtcgccagg aagaatacga cctcgtcgtg atcgcggagg gcgtcggctc ctccacccgc
       61 gagctggtct tcgccggcga gaaccggccg cgctggatcg acctgaccat gggatatttc
      121 acgatcccga aagcggccgg cgacagcgag gtcgcgcgat ggttcaccgc ggggggaggt
      181 cgcagcgtcc ttctgcggcc cgacaagcgc gggacaacgc gggcactcct gaactttcaa
      241 gcgccaccgc agggcgagga caggctcgag ctggcggccc agaaggcgct gctgcgccgg
      301 cgcttcgccg acgccggctg ggagaccccg cgcgtcctcg acggcctcga cagcagcacc
      361 gacttttata tggacgtact tcggcaggtg aagctcgcgc gctggtcgaa gggtcgcgtc
      421 gtgctcaccg gtgatgcggc gtggtgcgcg accccgctga gcgggatcgg cacgaccctc
      481 gcgatcgtcg gaccctacgt gctcgccggg gagctatgca aacaggcgga catcgccggg
      541 gccctggctg agtacgagcg gatcatgcgg ccttatgtcc acaaggggca ggacttcccc
      601 aagttcatgg cacgcatggc gcaaccccgg agccgcctcg gcgtgcggct gcaaggcgcc
      661 ctgctcggcg tcgcggccgc tccgggcctc cgcaagctcg tcgcaaagct gttctcgcct
      721 gccgccgacg acgtcgagct gcaggactat cgcatttgag gcccagccgc gacggggccc
      781 gccggcaccc cgacaagacc gtgaagttta tcaaagacac caacctcgag ttctacggcg
      841 ccgccggccc gctcgacccg tgacgggccg aaccaagcgc gtcagccggg catcagcgac
      901 gagttgaagc cgagcgtgac ccgcaggcgc ttgccgggct cgtcgacgtt gttgcccatg
      961 gtcgtgtgtg tcatcaggtc gccgtcgatc gcgttcggct ccgaacccgg cacgtaattg
     1021 tcgccgaggc cctggaaact ggcgaggaag ctgccgaact cgaccgagtc atcggcgtag
     1081 ttgaggctga tctccgcgag gttgaggccg ccgccggccg ggagcttggc gccgaacgag
     1141 cctgctatcg tcagatagct gccgtccggg ttgaagctgc cgtcctcctc tgaatagtag
     1201 acatcataga attcgacgct ggtcccctcg atgtcgcggt tcgcgttgct cagcgggtcg
     1261 gggagctgtt gattgagctc ggagccgtcg acggcgaagg tgtattccat caccccgctc
     1321 gtcgcctcat aaaaggtgat cgaggtcagc cccgggatgt cgtgcacggg gatcggcatg
     1381 ctctcgggca ccggcaggtc gagcttgaac cctcccgagg aggcgtcgcc ggacgtcgtc
     1441 gtcgcgtcgc cggtgctcgt gcccgccgcc atcatggtcg tatcgcccgt gctcgacggc
     1501 tcgacggtgc caccgatg
//
LOCUS       DASWNJ010001780         1749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_210126, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001780 DASWNJ010000000
VERSION     DASWNJ010001780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210126"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VGB85_29750"
     CDS             <1..455
                     /locus_tag="VGB85_29750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138320.1"
                     /translation="DLRMVDLPALRDQIALVRPGDLFHATILGNLRLGAPQVPLGALR
                     VAISLVDLADAIAALPAGLDTPLASDGAPLSRVQARRLVLARALAAQPRLLLIDGALD
                     GLQLPADKHEALLEHIFAADAPWTVLVVSDDPRVQARCRRVVDLARPS"
     gene            467..>1749
                     /locus_tag="VGB85_29755"
     CDS             467..>1749
                     /locus_tag="VGB85_29755"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY0138321.1"
                     /translation="MDITEPTILRLTMPTQTARTLARRLVQAALVLLALLVLAPWQQS
                     LPGHGRVIAYAPLDRLQALEAPMDGRVVEWMVEEGTHVAAGDPVARIADNDPDLLTRI
                     ERERRAAQDRVEAARRAIDLSERRIEGLELARLSATDGARMRAGMAVDRELAAQQAID
                     AAQAGLNAAILNLERTQKLAAEGLASTRSLELAELAEQTARADLLRARAAQAAARGEI
                     KAMRADTSRIDSDTGAAVDAAKVALETARGDLARAEQELARIESREVRQGAMVVTAPR
                     DGSLLRQVARQGGEMVKAGDPIAQFVPDTEARAVELWLDGIDAPLVTPGRHVRLQFEG
                     WPAVQFVGWPSVAVGTFGGVVAFVDATDDGKGHFRVVVLPDLAAPPEDHWPTAQYLRQ
                     GVRANGWILLDEVRLGYELWRQFNGFPPVLTGAPVG"
BASE COUNT          212 a          686 c          636 g          215 t
ORIGIN      
        1 tcgacctgcg catggtcgac ctgccggcgt tgcgcgacca gatcgcgctg gtgcgtccgg
       61 gcgacctgtt ccacgcgacg atcctcggca acctccgcct cggcgcgcca caggtgccgc
      121 tcggggcgct gcgcgtggcg atcagcctcg tcgacctcgc ggacgcgatc gcggcgctgc
      181 cggccggcct cgatacgccg ctcgcctcgg acggcgcgcc gctgtcgcgc gtgcaggccc
      241 ggcgcctcgt gctcgcgcgg gccctcgccg cccagccgcg cctgctgctg atcgacggcg
      301 cgctcgacgg tcttcagctg ccggccgaca agcacgaggc gctgctcgag cacatcttcg
      361 ccgccgacgc gccgtggacc gtgctcgtcg tcagcgacga cccgcgcgtg caggcccgct
      421 gccggcgggt cgtcgacctc gcaagaccct catgatccgg ggcgatatgg acatcaccga
      481 gccgaccatc ctgcgcctga ccatgccgac ccagaccgcg cgtacgctcg cgcgccgcct
      541 cgtgcaggcg gcgctggtgc tgctggcgct gctggtgctc gcgccgtggc agcagtcgct
      601 gccgggccac ggccgcgtga tcgcctacgc cccgctcgac cgcctgcagg ccctcgaggc
      661 gccgatggac ggccgcgtgg tcgaatggat ggtcgaggag ggcacccacg tcgccgccgg
      721 cgaccccgtg gcccgcatcg ccgacaacga ccccgacctg ctgacccgca tcgaacgcga
      781 gcgtcgcgcc gcgcaggacc gcgtcgaggc ggctcgccgg gcgatcgacc tcagcgagcg
      841 ccggatcgag ggcctcgagc tcgcgcggct ctcggccacc gacggggccc gcatgcgcgc
      901 cggcatggcc gtcgaccgcg agctcgccgc gcagcaggcg atcgacgccg cgcaggccgg
      961 cctcaacgcc gcgatcctca acctcgagcg gacccagaag ctcgccgccg agggcctcgc
     1021 ctcgacccgc agcctcgagc tcgccgagct cgccgagcag acggcgcgcg ccgacctgct
     1081 gcgcgcgcgc gcggcccagg cggccgcgcg cggcgagatc aaggcgatgc gcgccgacac
     1141 ctcgcggatc gacagcgaca ccggggcggc cgtcgacgcc gccaaggtcg cgctcgagac
     1201 ggcgcgcggc gatctggcgc gcgccgagca ggagctcgcg cgcatcgagt cccgggaggt
     1261 gcgacagggc gcgatggtcg tgacggcgcc gcgcgacggc agcctgctgc gccaggtcgc
     1321 tcgccagggc ggcgagatgg tcaaggccgg cgacccgatc gcccagttcg tgcccgacac
     1381 ggaggcgcgg gcggtcgagc tgtggctcga cggcatcgac gcgccgctgg tgaccccggg
     1441 ccgccacgtg cggctgcagt tcgagggctg gccggcggtc cagttcgtcg gctggccctc
     1501 ggtcgcggtc ggcaccttcg gcggcgtggt cgccttcgtc gacgcgaccg acgacggcaa
     1561 gggccacttt cgcgtcgtcg tgctgccgga cctcgcggcg ccgcccgagg atcactggcc
     1621 gacggcccag tacctgcgcc agggagtgcg cgccaacggc tggatcctgc tcgacgaggt
     1681 ccgcctcggc tacgagctgt ggcgccagtt caacggcttc ccgcccgtgc tcaccggcgc
     1741 cccggtcgg
//
LOCUS       DASWNJ010001781         4057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_491186, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001781 DASWNJ010000000
VERSION     DASWNJ010001781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491186"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VGB85_29760"
     CDS             complement(<1..621)
                     /locus_tag="VGB85_29760"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HEY0138322.1"
                     /translation="MFGRDRISLQGFNNLTKVVSFNLYDFYVARSEEERQRYVQDIDE
                     QFSASRITDILREIARIIDADVLSVSAQDYEPMGASSLVLMSDLGHTQNGGHPTASGA
                     GAGVGPASGHASVGAHLTKSHLCAHTYPDWTDPKGICSFRVDIDIATCGTIVPLRALD
                     YMFHSFENDVVIIDYVVRGFTRDAQGRRVYMDHTVKSIQDFIDPEIV"
     gene            863..3793
                     /locus_tag="VGB85_29765"
     CDS             863..3793
                     /locus_tag="VGB85_29765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138323.1"
                     /translation="MLGFSTAASFAFAALADDRIPNEEAIALSPTIGWLLLGCALCVY
                     LMFALQNERWRRFWLTNEDPRPVALFRIVFAFLCICNINDLFEYFEFLFTDEGIFLSD
                     AARQLVAAGQFKGFGDGIGDDPYGFFDASAWLEFLKGQKYSLLYFFDTPRAMWIQMLA
                     FYAVTVAFMVGWKSRVTGFLSFMLMNSFFLRNHLFWEGTELVYRVFFFYLLLARSGHA
                     YSVDNWLRTRRLRKEGRLSERDGPGGGAGLAPSPEHPRGLEAVYRLIPAWPRWLMVCN
                     LGVLYCYTGTVKNGAVWAKGDALYYALNMDHFFRFYPQEASSIFGLNLFRLATWITHW
                     WEALFPLMIVGLVTRWALREQLAPLQGWRLWAVRACWLGLALMAMVVVLVAFPVHYVQ
                     TPGGWSLERMQMFFAASWLALMAFIGGLWWALGTGKMAVRIRGRRYVLDRWWFCTWFL
                     GRRVWLTLGLMFHGNLQVLMNIGMFAPIMMSTYLFCFQGDEPGRVLRFFGRGLARLGI
                     PMPATIRRNEPPLPAEDRTLPWHIRDTRRLPEPLIYGLVALAVFGIWLQAGPLVPWLK
                     VAVFGYEAPVIAEGASAATIAAAELPGLHFGWTVVAIATILLVYTYIDGHRGTRGLAL
                     KVSALVVGLGAYLWLMGHEATDPVEAESLKFARVGVNLVIVAVLALQQIPWVHRHLAR
                     FGLTFSAPDRRPPETDRPITDEHSGRPRAPWAYRPGGRALAGFIIVYHITAIAFWELP
                     EKDSVSTFRIKGREAFSTWVMTTQTDQQWGMFAPNPPRHNVFMRIVLTDENGEQWDMR
                     SDVYAPERMPIPWIWNDRMRKMNRRVIGGESGKGDWYQKWYARYLCREWARTHRGVMP
                     QKVELFKLTYKMPSPEMVAKQGWYVPATLLHDTGREERQYTEKCANAIHGQLPNFIRE
                     RHGIEPLDEKGFKAWIKSKKKAWDRRLEPPAPKGKSETKTTPTGRVGSAARAARANEA
                     KATQ"
BASE COUNT          590 a         1492 c         1283 g          692 t
ORIGIN      
        1 cacgatctcc gggtcgatga agtcctggat cgacttcacc gtgtggtcca tgtacacgcg
       61 ccggccctgg gcgtcgcgcg tgaagccgcg gacgacatag tcgatgatga cgacgtcgtt
      121 ctcgaagctg tggaacatgt agtcgagcgc gcgcagcggc acgatcgtgc cgcaggtggc
      181 gatgtcgatg tcgacccgaa agctgcagat ccccttcggg tcggtccagt ccggataggt
      241 gtgcgcgcac aggtgactct tggtcaggtg cgccccaaca ctcgcgtggc cgctcgccgg
      301 gccaacacca gcgcctgcac ctgaagcagt cggatgcccg ccgttttgcg tgtgcccgag
      361 gtcgctcatc aacaccagcg agctcgcgcc catcggctcg tagtcctgcg ccgacaccga
      421 cagcacgtcc gcgtcgatga tcctggcgat ttcgcgcaag atgtcggtga tccgactcgc
      481 gctgaactgc tcgtcgatgt cctgcacgta gcgctggcgc tcctcctcgc tgcgcgcgac
      541 gtagaagtcg tagaggttga agctgacgac cttggtcagg ttgttgaacc cctgcaagga
      601 gatacggtcg cgcccgaaca tgggcgggga cgatggccgt ggacccctcg acagtcaagc
      661 cgcgtaaacg atgttcgcgc gggcccgccc gccgccggcg gaacacccgc gagcccggcc
      721 gcgggtcccc gaaggccacc tggagccgtc ccggtggctc caacggctcc ctgcgggcct
      781 ctcgctgcgg ttgccgggcc tcgccccgca attgcttttg cgcttgcgcc cctggcgggc
      841 cgtccccgac actcccaccc ctatgcttgg gttctccacg gccgcatctt ttgcgttcgc
      901 cgcgctggcc gacgaccgca tccccaacga ggaggcgatc gccctctcgc cgacgatcgg
      961 ctggttgctg ctgggctgcg ccctctgtgt ctacttgatg ttcgcgctgc aaaacgagcg
     1021 ctggcgccgc ttctggctga cgaacgagga cccgcgaccg gtcgccctgt ttcgcatcgt
     1081 cttcgccttc ctctgcatct gcaacatcaa cgacctgttc gagtacttcg agttcctgtt
     1141 caccgatgag ggcatcttcc tcagcgacgc cgcccgccag ctggtcgccg ccggtcagtt
     1201 caagggcttc ggcgacggca tcggcgacga cccctacggc ttcttcgatg cctccgcctg
     1261 gctcgaattc ctcaagggtc agaagtactc gctgctgtac ttcttcgaca cgccccgggc
     1321 catgtggatc cagatgctcg cgttctacgc ggtcaccgtg gcgttcatgg tcggctggaa
     1381 gtcgcgcgtc accgggttcc tgagcttcat gctcatgaac agcttcttcc tccgcaatca
     1441 cctgttctgg gagggcacgg agctggtcta tcgcgtgttc ttcttctacc tgctgctcgc
     1501 ccgcagcggc cacgcgtact cggtcgacaa ctggctgcgc acgcgaaggc tgcgcaaaga
     1561 gggccgcctc agcgagcgcg acggccccgg cggcggcgca ggactggccc cgagccccga
     1621 gcacccgcgc ggcctcgagg ccgtctaccg cctgatcccc gcgtggccgc gctggctgat
     1681 ggtgtgcaac ctcggcgtgc tctactgcta caccggcacg gtcaagaacg gcgccgtgtg
     1741 ggccaagggc gacgcgctgt actacgcgct caacatggac cacttcttcc ggttctaccc
     1801 gcaagaagcg tcctcgatct tcggcctcaa tctctttcgc ctcgccacct ggatcaccca
     1861 ctggtgggag gccctgttcc cgctgatgat cgtcggcctg gtcacccgct gggccctgcg
     1921 cgagcagctc gccccgctgc agggctggcg tctgtgggcc gtgcgcgcct gctggctcgg
     1981 cctcgcgctg atggcgatgg tcgtcgtgct cgtcgcgttc ccggtccact acgtccagac
     2041 cccgggcggc tggagcctcg agcgcatgca gatgttcttc gcggccagct ggctcgcgct
     2101 gatggcgttc atcggcggcc tgtggtgggc gctcggcacc ggcaagatgg cggtgcggat
     2161 ccgcggtcgc cgctacgtgc tcgaccgctg gtggttctgc acctggttcc tcggccgccg
     2221 cgtgtggctg accctcggcc tgatgttcca cggcaacctc caggtgctga tgaacatcgg
     2281 catgttcgcg ccgatcatga tgtcgacgta cctgttctgc ttccagggcg acgaaccggg
     2341 ccgggtcctg cgcttcttcg gccgcgggct cgcccgcctc ggcatcccga tgccggcgac
     2401 gatccgccgc aacgagccgc cgctgccggc cgaggaccgc accctgccgt ggcacatccg
     2461 cgacacccgc cgcctgcccg agccgctgat ctacggcctc gtcgccctcg ccgtgttcgg
     2521 catctggctg caagccggcc cgctggtccc ctggctcaag gtcgccgtgt tcggctacga
     2581 ggccccggtg atcgccgagg gcgcctcggc cgcgacgatc gccgccgccg agctccccgg
     2641 cctgcacttc ggctggacgg tcgtggcgat cgccacgatc ctgctcgtct acacgtacat
     2701 cgacggccac cgcggcaccc gcggcctcgc gctcaaggtg agcgcgctcg tcgtcgggct
     2761 cggcgcctac ctgtggctga tgggccacga ggcgaccgac ccggtcgagg ccgagtcgct
     2821 caagttcgcc cgcgtcggcg tcaacctcgt catcgtcgcg gtgctcgcgt tgcagcagat
     2881 cccgtgggtg caccggcacc tggcccgatt cggcctgacc ttcagcgccc ccgaccgccg
     2941 gccccccgag accgaccgcc cgatcaccga cgagcactcc ggccgccccc gcgccccctg
     3001 ggcctaccgc cccggcggcc gcgcgctcgc cggcttcatc atcgtctatc acatcaccgc
     3061 gatcgccttc tgggagctcc cggaaaagga ctccgtctcg accttccgca tcaagggtcg
     3121 cgaggcgttc tccacctggg tgatgaccac ccagaccgac cagcaatggg gcatgttcgc
     3181 ccccaacccg ccgcgccaca acgtgttcat gcgcatcgtc ctcaccgacg agaacggcga
     3241 gcagtgggac atgcgcagcg acgtctacgc ccccgagcgc atgccgatcc cgtggatctg
     3301 gaacgaccgc atgcgcaaaa tgaaccgccg tgtcatcggc ggcgagtcgg gcaagggcga
     3361 ctggtaccag aagtggtacg cccgctacct gtgccgcgaa tgggcccgca cgcaccgcgg
     3421 cgtgatgccg caaaaggtcg agctttttaa actgacctac aagatgccct cgcccgaaat
     3481 ggtcgccaaa cagggctggt acgtgcccgc cacgctgctg cacgacaccg gccgcgagga
     3541 gcgccagtac accgaaaagt gcgccaacgc gatccacggc cagctcccca acttcatccg
     3601 cgaacgccac ggcatcgagc cgctcgacga gaagggcttc aaggcgtgga tcaagtcgaa
     3661 gaaaaaggcg tgggacaggc gcctcgagcc gccggcgccc aagggcaagt cggagaccaa
     3721 gacgaccccc accggccgcg tcggctcggc cgcccgcgcc gcccgagcca acgaggccaa
     3781 ggccacccag tagatccccg caaggctccg atggtcgtgc tcggctcctt gatcgcgcgc
     3841 tcggtcgctc gcccgagcgt gtgcgccttc ccgagcgcgt gctcgagcga cgcggtcctc
     3901 gcctccccga gcgcgtgctc gagcgacgca gtcctcgcct ccccgagcgc gtgctcgagc
     3961 gacgcggtcc tcgcctcccc gaccgcgtgc tcgagcgacg cggggcgagg cttcatgggc
     4021 cggggggggc cgacggacgg cgggtccctg cgacgcg
//
LOCUS       DASWNJ010001782         4494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_383502, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001782 DASWNJ010000000
VERSION     DASWNJ010001782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383502"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2151
                     /locus_tag="VGB85_29770"
     CDS             <1..2151
                     /locus_tag="VGB85_29770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138324.1"
                     /translation="PAALLNVFVNGVAGTATSAGVVSFADGNPAAVIAGVVGAQAAVI
                     NEAGPPASKDLSLPPGGIALWSDPSEQVDAQLSAYIHANVVKQRVRSVAPDFAYLDQQ
                     LQIFVNIADICNAFSDGDNLNFFQSGAGCENTARIADVVYHEFGHSAHVQSLVPGVGQ
                     FEGALSEGIADYLSASITNDASLAPGFFLDSPGEPLRELDPQGDEWRWPDDLTGEVHD
                     DGRIIGGTLWDLRTALIDKLGPAGGVQADRIWFESIRRAVDIPTMYPEALVADDDDGN
                     LANGTPNECEINLAFNEHGLLGGASATGSVTLFPTTPNGTPVHLEIVGGSKACVDLAA
                     TGATLVVKTSGLVVDMIPEPGGFGAVLPNFPDGTLAEYQVVVNFNDGSTTSFPQNPAD
                     PWYQKYFGPVTNLFCTTFEGPPESEGWALQGQWEWGAATGQSGDPNAPFSGGGMVGTN
                     LAGSYAPFTASSMISPPISTAGFKTVRLQYRRWLTVEDAFFDQATISVNGAPVWQNLN
                     SDNGDLSTTAHRDSEWRFHDVDISGAAAGGQVQLEFALASDGGLEMGGWNVDEVCVVG
                     TEQGPPPSFCGDGIVDAGEACDNGPANSDTTPNACRTDCVFAFCGDAVIDGGEQCDDG
                     DALGGDGCSAACMIEGPSTTTDVPTTSASDAASVGDDDDGSDTDSESGSSGSGLEQID
                     LSDRGCACDGGEPRGLPALGLGLLVLAGLRRRRR"
     gene            2243..>4494
                     /locus_tag="VGB85_29775"
     CDS             2243..>4494
                     /locus_tag="VGB85_29775"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0138325.1"
                     /translation="MSDRPSTDVASSVLEILRDLITTVGDGRGLGNLGLDASLERELG
                     LGSLERVELLGRVEERFKLRLGEEAISVADTPRQIVRLIEAGLAQGGQAGASDMSSGT
                     GPKGQPRGLPTDEPAPVPVHARTLVEVLEYRAQHDGDRVHIMLLREDGSEDPITYGEL
                     RREAGAVAATLRGLGLRPGGTVAIMLPTGRGYFAAFLGSMLAGGVPVPLYPPFRLDRI
                     AEYIAREAKILDNAGTEVLLTFDRAARVADVVRDHVAAVKHVVSIEAILRDPSPMSSV
                     SAELSPDDTALLQYTSGSTGDPKGVQLTHANVLANVRASAAGCELTTRDVMVSWLPLY
                     HDMGLIGGWLMNFYYGNPTVLMSPVNFLSRPESWLHALSHYGGTVSCAPNFAFDLCVR
                     RIKPEVVATLDLSRARALLNGAEPILPATLDRFAGHLAPAGLRRGAIFCAYGLAENMV
                     GVTMPPVLRDPRIDTVDRRLFEREGRAVPVPEDSKADALHFVGCGHAMPTNEIRVVDK
                     QGEPVPDRVQGGLQFRGPSAFKGYYRNPEATARVKRPDGWNETGDLAYMVDGEVFITG
                     RVKDLIIKGGRNYYPHEIEAAAGSVPGIRQGCVAAFAMRDEHAGTESVVVVAETKERR
                     AEQRAQLIRAVGEAVVAAVGIPPDHVALTSPGAVPKTSSGKIRRGETRELFLAGTLGK
                     PHGSMYRQAAGLMLRGLPRRAAGLARGAAEVAYGGYALGAFGTLFAGALAVTPLVPPG
                     APLRRFTTRT"
BASE COUNT          638 a         1734 c         1534 g          588 t
ORIGIN      
        1 ccggcggcgc tgctcaacgt gttcgtcaac ggcgtcgccg gcacggccac ctccgccggc
       61 gtcgtcagct tcgccgacgg caacccggcg gcggtgatcg ccggggtcgt cggcgcgcag
      121 gcggcggtca tcaacgaggc cggcccgccg gcgagcaagg acctgtccct tccgccaggt
      181 ggcatcgcgc tgtggagcga cccgagcgag caggtcgacg cccagctgag cgcgtacatc
      241 cacgccaacg tcgtcaagca gcgggtgcgc tcggtcgccc cggacttcgc ctacctcgac
      301 cagcagctgc agatcttcgt caacatcgcc gacatctgca acgccttctc ggacggcgac
      361 aacctcaact tcttccagtc gggggccggc tgcgagaaca ccgcgcggat cgccgacgtc
      421 gtctatcacg agttcggcca cagcgcgcac gtgcagagcc tcgtgccggg cgtcgggcaa
      481 ttcgagggcg cgctgagcga gggcatcgcc gactacctct cggcctcgat caccaacgac
      541 gccagcctcg cgccgggctt cttcctcgac tcgcccggcg agccgctgcg cgagctcgac
      601 ccgcagggtg acgagtggcg ctggcccgac gacctgaccg gcgaggtcca cgacgacggc
      661 cgcatcatcg gcggcacgct ctgggacctg cgcaccgccc tgatcgacaa gctcggcccc
      721 gccggcgggg tccaggccga ccggatctgg ttcgagtcga tccgccgcgc ggtcgacatc
      781 ccgacgatgt accccgaggc cctcgtcgcc gacgacgacg acggcaacct cgccaacggc
      841 acgcccaacg agtgcgagat caacctcgcc ttcaacgagc acggcctgct cgggggcgcc
      901 agcgcgaccg gcagcgtgac cctgttcccg acgacgccca acggcacgcc ggtgcacctc
      961 gagatcgtcg gcggctccaa ggcctgcgtc gacctcgcgg cgaccggcgc gacgctcgtc
     1021 gtcaagacca gcggcctcgt cgtcgacatg atccccgagc ccggcggctt cggggcggtc
     1081 ctgccgaact tccccgacgg caccctcgcg gagtaccagg tcgtcgtcaa cttcaacgac
     1141 ggctcgacga ccagcttccc gcagaacccg gccgacccct ggtatcagaa gtacttcggg
     1201 ccggtcacca acctgttctg tacgaccttc gagggcccgc ccgagagcga gggctgggcg
     1261 ctccagggcc agtgggagtg gggcgccgcg accggacagt ccggcgatcc gaacgcgccg
     1321 ttctccggcg gcggcatggt cggcaccaac ctcgcgggct cctacgcgcc gttcaccgcc
     1381 agcagcatga tcagcccgcc gatcagcacc gccggcttca agaccgtgcg cctgcaatat
     1441 cgccgctggc tcaccgtcga ggacgcgttc ttcgaccagg cgacgatctc cgtcaacggc
     1501 gcgccggtgt ggcagaacct caacagcgac aacggcgacc tcagcaccac ggcccaccgt
     1561 gacagcgagt ggcgcttcca cgacgtcgac atctccggcg ccgcggcggg cgggcaggtc
     1621 cagctcgagt tcgccctcgc cagcgacggc ggcctcgaga tgggcggctg gaacgtcgac
     1681 gaggtgtgcg tcgtcggcac cgagcagggc ccgcccccgt cgttctgcgg cgacgggatc
     1741 gtcgacgccg gcgaggcctg cgacaacggc ccggccaaca gcgacaccac gccgaacgcg
     1801 tgccgcaccg actgcgtgtt cgccttctgc ggcgacgccg tgatcgacgg cggcgagcag
     1861 tgcgacgacg gcgacgcgct cggcggcgac ggctgcagcg cggcctgcat gatcgagggc
     1921 ccgagcacca ccacggacgt gccgaccacc agcgccagcg atgccgccag cgtcggcgac
     1981 gacgacgacg gcagcgacac cgacagcgag tcgggatcca gcggctccgg actcgaacag
     2041 atcgacctct ccgaccgcgg ctgcgcctgc gacggcggcg agccgcgcgg cctcccggcg
     2101 ctcggcctcg gcctgctcgt gctcgcgggc ctgcgccggc gccgtcgctg agggccaggg
     2161 gcctcgctcc cgggccgcgc aggcccgggc gccggcccgc gggcgtttgt gccctgcccc
     2221 gcaggttgtt accctcccgc gcatgtcgga ccgacccagc acggacgtgg cgagcagcgt
     2281 gctcgagatc cttcgtgacc tcatcaccac ggtcggcgac ggacgcggcc tcggcaacct
     2341 cggcctcgac gcctcgctcg agcgcgagct cgggctcggc agcctcgagc gcgtcgagct
     2401 gctcggccgc gtcgaggagc gcttcaagct gcgcctcggc gaggaggcga tcagcgtcgc
     2461 cgacacgccg cgccagatcg tgcgcctgat cgaggccggc ctcgcgcagg gcggtcaggc
     2521 tggtgcttcg gacatgtcct ccgggaccgg tcccaaaggc cagccccgcg gcctgccgac
     2581 cgacgagccc gcgccggtgc cggtgcacgc gcgcacgctc gtcgaggtgc tcgagtaccg
     2641 cgcccagcac gacggcgacc gcgtgcacat catgctgctg cgcgaggacg gctccgagga
     2701 cccgatcacc tacggcgagc tgcgccgcga ggccggcgcg gtcgcggcga cgctgcgcgg
     2761 cctcgggctg cgccccggcg gcacggtggc gatcatgctg ccgaccgggc gcggctactt
     2821 cgcggccttc ctcggctcga tgctcgccgg cggcgtgccg gtgccgctct acccgccgtt
     2881 tcgcctcgac cgcatcgccg agtacatcgc ccgcgaggcg aagatcctcg acaacgccgg
     2941 caccgaggtg ctgctgacct tcgaccgcgc cgcccgcgtc gccgacgtcg tccgtgacca
     3001 cgtcgccgcg gtcaagcacg tcgtctcgat cgaggcgatc ctgcgcgacc cgtcgccgat
     3061 gtcgtccgtg tcggccgagc tctcgcccga cgacaccgcc ctgctgcagt acacctccgg
     3121 cagcaccggc gaccccaagg gcgtgcagct gacccacgcc aacgtgctcg ccaacgtccg
     3181 cgcctcggcg gccggctgcg agctgacgac ccgcgacgtc atggtcagct ggctgccgct
     3241 ctaccacgac atgggcctga tcggcggctg gctgatgaac ttctattatg gaaatccgac
     3301 cgtgctcatg tcgccggtca acttcctctc gcgccccgag agctggctgc acgcgctgtc
     3361 acactacggc ggcaccgtct cgtgcgcgcc caacttcgcg ttcgacctgt gcgtgcggcg
     3421 gatcaagccc gaggtggtcg ccacgctcga cctgtcgcgc gcgcgcgccc tgctcaacgg
     3481 cgccgagccg atcctgccgg cgacgctcga ccgcttcgcc ggccacctcg cgcccgccgg
     3541 cctgcgccgc ggggcgatct tctgcgccta cggcctcgcc gagaacatgg tcggcgtgac
     3601 gatgccgccg gtgctgcgag acccgcggat cgacaccgtc gaccggcgcc tgttcgagcg
     3661 cgagggccgg gccgtacctg tccccgagga cagcaaggcc gacgccctgc acttcgtcgg
     3721 ctgcggccac gcgatgccga ccaacgagat ccgcgtcgtc gacaagcagg gcgagccggt
     3781 gccggaccgc gtgcagggcg gcctgcagtt tcgcgggccc tcggcgttca agggctacta
     3841 ccgcaacccc gaggcgaccg cccgggtcaa gcggcccgac ggctggaacg agaccggcga
     3901 cctcgcgtac atggtcgacg gcgaggtgtt catcaccggc cgcgtcaagg acctgatcat
     3961 caagggcggc cgcaactact acccgcacga gatcgaggcc gcggccgggt cggtgccggg
     4021 gatccgccag ggctgcgtcg ccgcgttcgc catgcgcgac gagcacgcgg gcacggagtc
     4081 ggtggtcgtc gtcgccgaga ccaaggagcg gcgcgccgag cagcgcgcgc agctgatccg
     4141 ggccgtcggt gaggcggtgg tcgccgcggt cggcatcccg ccggaccacg tcgccctcac
     4201 ctcccccggc gcggtgccca agacctcgag cggcaagatc cgccgcggcg agacgcgcga
     4261 gctgttcctc gccggcacgc tcggcaagcc ccacggatcg atgtaccggc aagcggccgg
     4321 cctgatgctg cgcgggctgc cgcgtcgcgc cgccggcctg gcccgcggcg ccgccgaggt
     4381 cgcatacggc ggctacgcgc tcggggcctt cggcacgctg ttcgccgggg cgctcgcggt
     4441 cacgccgctg gtcccccccg gcgcgccgct gcgccgattc acgacgcgca cctg
//
LOCUS       DASWNJ010001783         9995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_316005, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001783 DASWNJ010000000
VERSION     DASWNJ010001783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 9995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9995
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316005"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            378..869
                     /locus_tag="VGB85_29780"
     CDS             378..869
                     /locus_tag="VGB85_29780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138326.1"
                     /translation="MTGFGGPVLQLSGLDRKFAAIVGIAVGATIKRRVSIGAAAFRLL
                     NPSDAGPTSVGAPQRLDFTYGALLVAVVFARTQRVDFTVEGLIGGGGACLQNPNNGSC
                     YARTAMFVGQPGLAARITLAPIVRIALGLGYRFVAARSWSGPGDARLGAPVGSVMLEF
                     GLF"
     gene            complement(898..2289)
                     /locus_tag="VGB85_29785"
     CDS             complement(898..2289)
                     /locus_tag="VGB85_29785"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0138327.1"
                     /translation="MTRLCATLAALGILSAHGCFKDEGPGQDPDTGTSTSGSTGAPTG
                     EPAVCGDGVVAGDEQCDAGELNAIFGACGLDCTLNVCGDGVPGPAEDCDDGNGNDGDA
                     CRNTCERARCGDGVVQDGELCDDGDLDEDDGCTSKCAPAACGDGVISGDEACDLAERN
                     ADDGLCTSMCASATCGDGLVQPGEACELGIASCGMDCRWTTCNNMMDDPGESCDGEAK
                     ECTDFCTAPRCGDGFTSLSEACDDGNGLDGDDCTASCQRSICGDGIVADDEPCDDMNA
                     ISGDGCGTSCTRDALFVFVSSETYLGAQVGGLAGADEHCRLLASKADLPGTYRAWLSD
                     GEASPATRFTKGKLPYVLPPGLHGLGVRVARNWVDLVDGTLDRPINVTETGELLVPGE
                     SCGAPALLAWTHTGPTAGPQEANASCGGWKFSINSVGSAGMVNRSDAAWTEGCGEVVC
                     TMALHLYCIEQRP"
     gene            complement(2355..3335)
                     /locus_tag="VGB85_29790"
     CDS             complement(2355..3335)
                     /locus_tag="VGB85_29790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138328.1"
                     /translation="MLHHPRLCSLMLVALGCSFDATGLGESTGAPGPATTGDASSGAP
                     PGTTTTAPDPTTGVTTTTVPVGATSDGSGTASTTETTTAPPVCGDGVLDPGEACDDGP
                     DNGPNRPCTPACGVNVCGDGYPLDPGEVCDDGNQDDADACRNDCTASPTCGNGKLDAG
                     EQCDDSNQLDTDACIVCKKAVCGDGFVQQNVESCDDGLESATCNADCSLVVCGDSKLN
                     NSAGELCDLGVKNGVYASGCGADCKSKGPYCGDGVVTAPDENCDPQAPLPSATCTTDC
                     KLLICAAGHGDCDDEFANGCEIDLQTDEANCGKCDKKCQVLDCKDGSCKP"
     gene            complement(3365..4339)
                     /locus_tag="VGB85_29795"
     CDS             complement(3365..4339)
                     /locus_tag="VGB85_29795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138329.1"
                     /translation="MHRPTLIARASALAAATLLACSFDATGLGDEVGGSPASGQTNAT
                     QNPASATDAAGSSSTGDAPDGTGTGASDPSTTTTEPPASCGDDVQDPGEECDAGPNNH
                     SGKACTAACKINVCGDGYFGLGEDCDPGPDPQNGQTCTPGCTINVCGDGYIGAGETCD
                     DGPNNGPDKACTDACTAAKCGDGKVGVGETCDDTNTNNNDACLNTCQSATCGDGFLQQ
                     GVESCDEGPENGTYGAPCNVTCNGSGPRCGDGHWDKVDEQCDGNDRPGGVTCDAACKA
                     ICTDKHADCNGNIGDGCEELQYNEDHCGMCNKPCNGPGNFCWQGDCAY"
     gene            complement(4345..5709)
                     /locus_tag="VGB85_29800"
     CDS             complement(4345..5709)
                     /locus_tag="VGB85_29800"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0138330.1"
                     /translation="MHSHAPRTAALARSIVLAMAGGCFADPGPVGSGNDETGATGTST
                     TTASDDSTGAPTGEPTTDGPADCGNSQIDAGEACDNGELNNGQGGGICKADCTLNTCG
                     DGYVATSEGCDDGNAIDDDACDNSCKLTGCGDGKLGPGEQCDDGDTVDNDGCSNLCKL
                     PFCGDGNLGEGEGCDDGPDNGDDQACTLDCTTAVCGDGKLLVGVEACDDGNQNDADAC
                     SNMCQQATCGDMIVQPNEDCDDGNMVDDDGCSNICSTLCGNAFLDPGEACDDGNNLDG
                     DTCSPTCQRTGFLVFVTSQKFSGDLGGVQGADAKCNALAASAGLPGAGHYRAWLSSDT
                     TAPFDSFVHSNLPYRLPDGQQVADNWSDMTTSSLLHPISRTEVGNSLAGDPECDNDDA
                     RVWTATTPTGLLKLEAHCDGWTKGGNLAQGGLGSALQIGPGWTDACGMSCGVMARLYC
                     FEQP"
     gene            complement(5712..7274)
                     /locus_tag="VGB85_29805"
     CDS             complement(5712..7274)
                     /locus_tag="VGB85_29805"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0138331.1"
                     /translation="MHPPALRTAALARSIVLTTVMAGGCFYNPAGSESEPTGSTGEST
                     TTASDASTGATTPTEPTTGGLLDCGDSQIDAGEECDNGELNNGQGGGICKADCTLNTC
                     GDGYVASNEGCDDGNSNDKDDCDNGCQLTGCGDGVPGPGEACDDANMVDDDGCSNLCK
                     LPFCGDGHVGMDEQCDDGPLNANDQACKLDCTAAACGDGDAWLAVEECDDGDQDDTNA
                     CTNACKLATCGDMIVSPAIGEQCDLGGMNSDLGACTAICKNAGCGDGLVQAGVEECDD
                     GNMVDDDTCSVACKLAKCGDAIVQAGEDCDDGNLAEDDGCTNTCKQLCGNGTLDPGED
                     CDDGNDNNDDICNNTCEKVALVVFVTSQKFPGDLGGMAGASEICNSLAAAAGLTGTYK
                     AWLSDFDDNPAQNFAQAMMPWRRLDGMTVASDWMDLITGSLGTPISVTETGMSVGGGN
                     NDCSNPERVVWTNTKPAGILDNEPHCNGWTSAQANDKAGGGVAGSVSAQWTDACQLIC
                     NAQARLYCFEQP"
     gene            complement(7282..8913)
                     /locus_tag="VGB85_29810"
     CDS             complement(7282..8913)
                     /locus_tag="VGB85_29810"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0138332.1"
                     /translation="MRLHLLVAAATLSLTGCTFDATGLGAESSGVPITTTGDESTGSA
                     TTTTTDPTEDPTSTTGTPASCGNSALDDGEECDNGSANGQGSSCKADCTLNVCGDGYV
                     ASNEGCDDGNSNNEDDCSNDCKLTGCGDGMLGPGEECDDGNKVNTDGCSDLCKLPFCG
                     DGNVGMGEQCDDGTMNGDDKACKLDCTLAACGDGKTGPDEQCDDGNQMDDDGCANDCT
                     LTACGDGKPGPGEECDDGNQTEEDGCTNGCKLPSCGDMFVQAGEECDMGVMNSDMGAC
                     TTTCKKATCGDMFVQAGEDCDLGAMNNDMGACTTTCKSPKCGDTFVQAGEECDDGNTV
                     DDDACNNMCKQPCGNGMVEDAEECDDGNDNNDDICSNDCKKVALIVFVTSDKFQGDFG
                     GTSGANATCNTLAGAAGLTGTFKAWISDADDNPVKNFAMTTMPWRRLDGMTVASNWMD
                     LITGSLGVPISVTETGMSVGGGNNDCGNAEKVVWTSTKPNGTPDGKGHCNGWMSAQSN
                     DKAGAGVAGNVNATWTDACQPSCDTEARLYCFEQP"
     gene            complement(9141..>9995)
                     /locus_tag="VGB85_29815"
     CDS             complement(9141..>9995)
                     /locus_tag="VGB85_29815"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0138333.1"
                     /translation="TPVGSALGYILGGYIGEFYGWRAVFLVGGLPGLLFALMAFLMVE
                     PRRGQHDDEVLPAAGLSLPATLRRLIRTPAWRINTVGLALMTFTIGGLALWMPAYLER
                     VHGMSSGAAGTTFGGITVLAGVIGTLAGGRLGDRMQARSAGGYFRLSGIGLLCSSPFI
                     VLMTMLDSLPAIFAVVFVAELFLFLNTGPLNAALIGSVPVQMRGAAFAVNVLCIHAFG
                     DALSNVLLGVVSDAATPVLGSAAAGLSLAVAVTAIPVAIAGLLLLRGARWIARQPQGL
                     MTYADPRE"
BASE COUNT         1221 a         3450 c         3565 g         1759 t
ORIGIN      
        1 agcgcttccc tcgctggccc cccgcggccg gctccggcca ccccggtttt gggcccagcg
       61 tggccttgac tcgtgggtcc gcgggcggcc tacttcgagc gccctcgatg ctccgcgcga
      121 ctttttgcct cgccttccag ctgctcgccc cgacggccac acgcgagcat gacgggccca
      181 cggaggttca ggaacccgaa cggaggcccg tcgaggtaca cgagggttcg ccggctcctg
      241 gagcctcgtc ggagccggca gctcctgcgc ccacagccga ctccgtgaga tcggatccaa
      301 agccggcccc gaggtccgat ggaggtccga ccgccacgcc gcggccgaag tggtggggtc
      361 cgtatccgcg gccgaaggtg acgggcttcg gcgggccggt gctccagctg agcgggctcg
      421 accgaaagtt cgcggcgatc gtcgggatcg ccgtcggcgc gacgatcaag cgccgcgtgt
      481 cgatcggcgc cgcggcgttt cgcctgctca acccgagcga cgccggtccg acctcggtcg
      541 gcgcgccgca gcggctcgac ttcacctacg gcgcgctgct cgtcgccgtg gtgttcgcgc
      601 gcacgcagcg ggtcgacttc accgtcgagg gcctgatcgg cggcggcggg gcgtgcctgc
      661 agaaccccaa caacggctcg tgttacgcgc gcaccgcgat gttcgtcggc cagcccgggc
      721 tcgccgcgcg catcaccctc gcaccgatcg tccgcatcgc gctcgggctc ggctatcgct
      781 tcgtcgccgc gcggagctgg tccggacccg gcgacgcccg cctcggcgcg ccggtcggca
      841 gcgtcatgct cgagtttgga ttattttaaa aaggggacat gtcctttggg acatgtctca
      901 ggggcgctgt tcgatgcagt acaggtgcag ggccatcgtg cacacgacct cgccgcagcc
      961 ctcggtccag gcggcgtcgc tgcggttgac catgccggcg gagccgacgc tgttgatgct
     1021 gaacttccag ccgccgcagc tggcgttggc ctcctggggg cctgcggtcg ggccggtgtg
     1081 ggtccaggcg agcagggcgg gtgcgccgca ggactcgccg gggacgagca gctcgccggt
     1141 ttcggtcacg ttgatcggtc ggtcgagggt gccgtcgacg aggtcgaccc agttgcgggc
     1201 gacgcggacg ccgaggccgt ggaggccggg cgggaggacg tagggcagct tgcccttggt
     1261 gaagcgggtg gcggggctgg cctcgccgtc gctgagccag gcgcggtagg tgccgggcag
     1321 gtcggccttg ctggcgagca gccggcagtg ctcgtcggcg ccggcgagcc cgccgacctg
     1381 ggcgcccagg taggtctcgc tcgagacgaa cacgaacagc gcgtcgcggg tgcaggacgt
     1441 gccgcagccg tcgccggaga tcgcgttcat gtcgtcgcag ggctcgtcgt cggcgacgat
     1501 gccgtcgccg catatcgagc gctgacagct cgcggtgcag tcgtcaccgt cgaggccgtt
     1561 gccgtcgtcg caggcctcgc tcaggctcgt gaacccgtcg ccgcagcgcg gggccgtgca
     1621 aaagtccgtg cactccttgg cctcgccgtc acaagactcg cccgggtcgt ccatcatgtt
     1681 gttgcaggtg gtccagcggc agtccatccc gcagctcgcg atgccgagct cgcaggcctc
     1741 gcccggctgg accagcccgt cgccgcaggt cgcgctcgcg cacatgctcg tgcacaggcc
     1801 gtcgtccgcg ttgcgctcgg cgaggtcgca cgcctcgtcg ccgctgatca ccccgtcgcc
     1861 gcaggccgcc ggggcgcact tgctggtgca gccgtcatcc tcgtcgaggt cgccgtcgtc
     1921 gcacagctcg ccgtcctgca ccacgccgtc gccgcagcgc gcccgctcgc aggtgttgcg
     1981 gcaggcgtcg ccgtcgttgc cgttgccgtc gtcgcagtcc tccgcggggc caggcacgcc
     2041 gtcgccgcag acgttgagcg tgcagtcgag cccgcaggcg ccgaagatcg cgttgagctc
     2101 gccggcgtcg cactgctcgt cgccggcgac gaccccgtca ccacacacgg cgggctcacc
     2161 ggtcggcgcg ccggtcgagc ccgacgtgct cgtgccggtg tccggatcct gcccgggacc
     2221 ctcatccttg aagcagccgt gggcgctcag gatcccgaga gcagcgagcg tcgcgcagag
     2281 ccgggtcatg cggggcacag gcaagcatcg ccgggccgtg aaagcaagtg gcatgcaagc
     2341 gcgcacacgc tcgttcatgg tttgcagctg ccatccttgc agtccaagac ctgacatttc
     2401 ttgtcgcact tgccgcagtt ggcctcgtcg gtctgcagat cgatctcgca gccgttggcg
     2461 aattcgtcgt cgcagtcgcc gtgaccggcc gcgcaaatga gcagcttgca gtcggtcgtg
     2521 cacgtcgcgc tcggtagcgg cgcttgcggg tcgcagttct cgtccggcgc ggtgacgacg
     2581 ccgtcgccgc agtacgggcc cttgctcttg cagtccgcgc cgcagccgct cgcatacacg
     2641 ccgttcttga cgccgaggtc gcagagctcg cccgcgctgt tgttgagctt cgagtcgccg
     2701 cacacgacga gcgagcagtc ggcgttgcag gtcgcgctct cgaggccgtc gtcgcagctc
     2761 tcgacgtttt gttgcacgaa gccgtcgccg cagacggcct ttttgcacac gatgcaggcg
     2821 tcggtgtcga gctggttgct gtcgtcgcac tgctcgcccg cgtcgagctt gccgttgccg
     2881 caggtcggcg aggccgtgca gtcgttgcgg caggcgtcgg cgtcgtcctg gttgccgtcg
     2941 tcgcagacct cgccggggtc gagcgggtag ccgtcgccgc agacgttgac gccgcacgcc
     3001 ggcgtgcacg gccggttcgg gccgttgtcg gggccgtcgt cgcaggcctc gccggggtcg
     3061 agcacaccgt cgccgcagac cggaggcgcg gtcgtcgtct ccgtggtgga ggcggtcccg
     3121 ctgccgtccg aggtcgcgcc gaccgggacg gtggtcgtgg tcacgccggt cgtcgggtcg
     3181 ggggcggtcg tcgtcgtccc tggcggcgcg ccgctgctcg cgtctcccgt ggtcgcgggg
     3241 ccgggcgcgc cggtgctctc gccgaggccg gtggcgtcga agctgcatcc cagcgcgacg
     3301 agcatcagcg agcacaggcg aggatgatgg agcatgagca ctcccggcgc ggcgccggac
     3361 ggggtcagta ggcgcagtcg ccctgccagc agaagttccc cgggccgttg catggcttgt
     3421 tgcacatgcc gcagtggtcc tcgttatatt gcagctcctc gcagccgtcg ccgatgttgc
     3481 cgttgcagtc ggcgtgcttg tcggtgcaga tcgccttgca ggcggcgtcg caggtgacgc
     3541 cgccggggcg gtcgttgccg tcgcactgct cgtcgacctt gtcccagtga ccatcaccgc
     3601 accgggggcc ggagccgttg caggtgacgt tgcacggtgc gccatacgtg ccgttctcgg
     3661 ggccctcgtc gcagctctcg acgccctgct gcaagaagcc gtcgccgcag gtggccgact
     3721 ggcaggtgtt gaggcaggcg tcgttgttgt tggtgttcgt gtcgtcgcag gtctcgccga
     3781 cgccgacctt gccgtcgccg cacttggcgg cggtgcacgc gtcggtacac gccttgtcgg
     3841 ggccgttgtt ggggccgtcg tcgcaggtct cgccggcgcc gatgtagccg tcgccgcaga
     3901 cgttgatggt gcacccgggc gtgcaggtct ggccgttctg cgggtcgggc ccggggtcgc
     3961 agtcctcgcc gaggccgaag tagccgtcgc cgcagacgtt gatcttgcaa gccgcggtgc
     4021 aggccttccc gctgtggtta ttcggaccgg cgtcgcactc ctcgcccgga tcctggacgt
     4081 cgtcgccgca gctcgcgggc ggctcggtgg tcgtggtgga gggatccgac gcgccggtgc
     4141 cggtgccgtc gggcgcgtcg ccggtggagg aggagcccgc ggcgtcggtg gcgctggccg
     4201 ggttctgggt ggcgttcgtc tggcccgagg ccggcgagcc gccgacctcg tcgccgaggc
     4261 cggtcgcgtc gaagctgcac gcgagcagcg tggcggcggc gagcgcggag gcgcgggcga
     4321 tgagggtggg gcgatgcatg gcgatcacgg ctgttcgaag cagtacaggc gggccatcac
     4381 cccgcagctc atcccacagg cgtcggtcca gcccgggccg atctgcaggg cgctaccgag
     4441 gccgccctgg gcgaggttgc cacccttggt ccagccgtcg cagtgcgcct cgagcttcag
     4501 gagccccgtc ggcgtggtgg cggtccacac ccgcgcgtcg tcgttgtcgc actccgggtc
     4561 gcccgcgagg ctgttgccga cctcggtgcg ggagatcggg tgcagcaggc tgctcgtcgt
     4621 catgtcgctc caattgtcgg cgacctgctg accgtccggg agacgatagg ggaggttgga
     4681 gtggacgaag gagtcgaacg gcgcggtggt gtcgctgctg agccatgcgc ggtagtggcc
     4741 cgcgccgggg aggccggcgc tggcggccag agcgttgcac ttggcgtcgg cgccctggac
     4801 cccgccgagg tcgcccgaga acttctggct ggtcacgaac acgaggaacc cggtccgctg
     4861 gcaagtcggg ctgcacgtgt cgccgtcgag gttgttgccg tcgtcgcacg cctcgccggg
     4921 gtcgaggaac gcgttgccgc acagggtcga gcagatgttc gagcagccgt cgtcgtcgac
     4981 catgttgccg tcgtcgcagt cctcgttcgg ctgcacgatc atgtcgccgc acgtcgcctg
     5041 ctggcacatg ttcgagcagg cgtcggcgtc gttctggttg ccgtcgtcgc aggcctcgac
     5101 gccgaccagg agcttgccgt cgccgcagac cgccgtcgtg cagtcgagcg tgcacgcctg
     5161 gtcgtcgccg ttgtcggggc cgtcgtcgca gccctctccc tcgccgaggt tgccgtcgcc
     5221 gcaaaacggc agcttgcaca ggttgctgca tccgtcgttg tcgacggtgt cgccgtcgtc
     5281 gcactgctcg ccgggcccga gcttgccgtc gccgcagccg gtcaatttac agctgttgtc
     5341 gcaggcgtcg tcgtcgatgg cgttgccgtc gtcgcagccc tcgctcgtgg cgacatagcc
     5401 gtcgccgcag gtgttgaggg tgcagtcggc cttgcagatg ccgccgccct gaccgttgtt
     5461 gagctcgccg ttgtcacacg cctcgcccgc gtcgatctgc gagttgccgc aatccgccgg
     5521 cccgtcggtg gtcggctcgc ccgtcggcgc gccggtcgag tcgtcgctcg ccgtcgtggt
     5581 cgaggtgccc gtcgcgccgg tctcgtcgtt cccggagccc accggccccg ggtcggcgaa
     5641 gcagccgccg gccatcgcaa ggacgatgga gcgggcgagc gcggccgtgc ggggagcgtg
     5701 tgagtgcatg gctacggctg ttcgaagcag tacaggcgcg cctgggcgtt gcagatgagc
     5761 tggcaggcgt cggtccactg ggcgctgacg ctgcccgcga cgccgccccc ggccttgtcg
     5821 ttggcttgcg cgctggtcca gccgttgcag tgcggctcgt tgtcgaggat cccggccggc
     5881 ttggtgttgg tccacaccac cctctcgggg ttgctgcaat cgttgttgcc gccgccgacc
     5941 gacatgccgg tctcggtcac ggagatcggt gtgccgaggc tgccggtgat gaggtccatc
     6001 cagtcgctgg cgaccgtcat gccgtcgagg cgccgccacg gcatcatcgc ctgggcgaag
     6061 ttctgggccg ggttgtcgtc gaaatcgctg agccacgcct tgtaggtgcc ggtcagcccg
     6121 gcggcggcgg cgaggctgtt gcagatctcg ctggcccctg ccatgccgcc gaggtcgccc
     6181 gggaacttct ggctggtcac gaacacgacc agggcgacct tctcgcaggt gttgttgcag
     6241 atgtcgtcgt tgttgtcgtt gccgtcgtcg cagtcctcgc cgggatcgag cgtcccgttg
     6301 ccgcacagct gcttgcacgt gttcgtgcag ccgtcgtcct cggccaagtt gccgtcgtcg
     6361 cagtcctcgc cggcctgcac gatcgcgtcg ccgcacttgg ccagcttgca cgcgacggag
     6421 cacgtgtcgt cgtcgaccat gttgccgtcg tcgcactcct cgacgcctgc ctgcacgagc
     6481 ccgtcgccac acccggcgtt cttgcatatc gcggtgcagg cgccgaggtc gctgttcatg
     6541 ccgccgaggt cgcactgctc gccgatcgcc ggcgagacga tcatgtcgcc gcaggtcgcc
     6601 agcttgcacg cgttggtgca cgcgttggtg tcgtcctggt cgccgtcgtc gcactcctcc
     6661 accgcgagcc aggcgtcgcc gtcgccgcag gccgcggccg tgcagtcgag cttgcacgcc
     6721 tgatcgttcg cgttcaaggg gccgtcgtcg cactgctcgt ccatgccgac gtggccatcg
     6781 ccgcaaaacg gcagcttgca caggttgctg cagccgtcgt cgtcgaccat gttggcgtcg
     6841 tcgcacgcct cgccgggtcc gggcacgccg tcgccgcaac cggtcagctg gcagccgttg
     6901 tcgcagtcgt ccttgtcgtt gctgttgccg tcgtcgcagc cctcgttcga cgcgacgtag
     6961 ccgtcgccgc aggtgttgag ggtgcagtcg gccttgcaga tgccaccacc ctgaccgttg
     7021 ttgagctcac cgttgtcaca ctcctcgccc gcgtcgatct gcgagtcgcc gcaatcgagg
     7081 aggccgcccg tggtcggttc ggtgggggtc gtggcgccgg tcgaggcatc gctcgccgtc
     7141 gtggtcgact cgcccgtcga gcccgtgggc tcgctctccg agcctgccgg gttgtagaag
     7201 cagccgccgg ccatcaccgt cgtgaggacg atggaccggg cgagcgcggc cgtgcggagg
     7261 gcgggtgggt gcatgggtcg tctacggctg ttcgaagcag tacaggcgcg cctcggtgtc
     7321 gcagctgggc tggcaggcgt cggtccaggt ggcattgacg ttgcccgcga cgccggcccc
     7381 ggccttgtcg ttggactgcg cgctcatcca gccgttgcag tgccccttgc cgtcaggggt
     7441 cccgttcggc ttggtgctgg tccacaccac cttctcggcg ttgccgcaat cgttgttacc
     7501 gccgccgacc gacatgccgg tctcggtcac ggagatcggg acaccgaggc tgccggtgat
     7561 caggtccatc cagttgctgg cgaccgtcat gccgtcgagg cgccgccacg gcatcgtggt
     7621 catcgcgaag ttcttgaccg ggttgtcgtc ggcgtcgctg atccaggcct tgaaggtgcc
     7681 ggtcagcccg gcggccccgg cgagggtgtt gcaggtcgcg ttggcccccg atgtgccgcc
     7741 gaagtcgccc tggaacttgt cgctggtcac gaacacgatc agggcgacct ttttgcagtc
     7801 gttgctgcag atgtcgtcgt tgttgtcgtt gccgtcgtcg cactcctcgg cgtcctcgac
     7861 catcccgttg ccgcacggct gcttgcacat gttgttgcag gcgtcgtcgt cgaccgtgtt
     7921 gccgtcgtcg cactcctcac cggcctgcac gaacgtgtcg ccgcacttgg ggctcttgca
     7981 cgtcgtggtg caggcgccca tgtcgttgtt catggcgccg aggtcgcagt cctcgccggc
     8041 ctgcacgaac atgtcgccgc acgtggcttt tttgcacgtc gtggtgcagg cgcccatgtc
     8101 gctgttcatg acgcccatgt cgcactcctc gccggcctgg acgaacatgt cgccgcagga
     8161 cggcagcttg cacccgttgg tgcacccgtc ctcctccgtc tggttgccgt cgtcgcactc
     8221 ctcgccagga cccggcttgc cgtcgccgca ggccgtcagc gtgcagtcgt tggcgcagcc
     8281 gtcgtcgtcc atctggttgc cgtcgtcgca ctgctcgtcc ggccccgtct tgccgtcgcc
     8341 gcaggccgcc agcgtgcagt cgagcttgca cgccttgtcg tcgccgttca tggtcccgtc
     8401 gtcacactgc tcgcccatgc cgacgttgcc gtcgccgcag aacggcagct tgcacaggtc
     8461 gctgcacccg tcggtgttga ccttgttgcc gtcgtcgcac tcctcgcccg gcccgagcat
     8521 gccgtcgccg cagccggtca gcttgcagtc gttgctgcag tcgtcctcgt tgttgctgtt
     8581 gccgtcgtcg cagccctcgt tcgaggcgac gtaaccatcg ccgcacacgt tgagggtgca
     8641 gtccgccttg caactgctgc cctggccgtt ggccgagccg ttgtcacact cctcgccgtc
     8701 gtcgagcgcc gagttgccgc agctcgccgg ggtgcccgtc gtgctcgtcg gatcttccgt
     8761 cgggtcggtc gtggtggtgg tcgccgaacc ggtgctctcg tcgccggtgg tggtgatggg
     8821 gacgccgctg ctctccgcgc cgaggcccgt cgcatcgaag gtgcatccgg tcagcgagag
     8881 ggtggcggcc gccacgagga ggtgaaggcg catgatgcga gttcctgaca tcttggagat
     8941 aaggaggaaa aaccggcgaa aaggcgcggc cataaggccc cgccgacgcg tcggaacacg
     9001 atacgagagc cgcgatgggg ctgtccaccg gggccgtcac cgatcgaccg aggcctgtgg
     9061 ccgacagcgc gggcggatct gctcgcgact gcacatgtct cggagaacag gccaggaagt
     9121 gcgcagtacc tgggccgcgg tcactcgcgt ggatccgcgt aggtcatcag gccctgcggc
     9181 tgccgggcga tccagcgcgc gccccggagc aacagcaggc ctgcgatcgc gacggggatc
     9241 gcggtgaccg ccaccgcgag gctcaggccg gcggcggcgc tgccgagcac cggcgtcgcg
     9301 gcgtcgctga cgacgccgag caggacgttc gagagcgcgt cgccgaaggc gtggatgcac
     9361 agcacgttga ccgcgaacgc ggctccgcgc atctgcacgg ggacgctgcc gatcagcgcc
     9421 gcgttgagcg ggccggtgtt gaggaacagg aacagctcgg cgacaaagac gaccgcgaag
     9481 atcgccggca gcgagtcgag catcgtcatg aggacgatga acgggctgct gcacagcagg
     9541 ccgatcccgc tcaggcgaaa gtacccgccg gccgagcgcg cctgcatgcg atcgccgagg
     9601 cgtccgccgg cgagcgtgcc gatcacgccc gcgagcaccg tgatcccgcc gaaggtggtg
     9661 ccggccgcgc ccgacgacat gccgtgcacg cgctcgaggt aagcgggcat ccacagcgcg
     9721 agcccgccga tcgtgaaggt catcagcgcc aggccgaccg tgttgatccg ccacgccggg
     9781 gtccgtatca ggcggcgcag cgtcgccggc agcgacaggc cggcggcggg caggacctcg
     9841 tcgtcgtgct ggcccctgcg cggctcgacc atcaagaagg ccatcagcgc gaacagcagc
     9901 cccggcaggc cgccgaccag gaacaccgcg cgccagccat aaaactcgcc gatgtagccg
     9961 ccgaggatgt agccgagcgc cgagccgacc ggcgt
//
LOCUS       DASWNJ010001784         9561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_243625, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001784 DASWNJ010000000
VERSION     DASWNJ010001784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 9561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9561
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243625"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VGB85_29820"
     CDS             complement(<1..344)
                     /locus_tag="VGB85_29820"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEY0138334.1"
                     /translation="MAWKFLGTGWQAPLTFVAGRLQTVSDEACVEQAIWAILETAPGE
                     RMMQPEFGCAIHDLVFAAANPTTIGRIHGAVHGALTRFEPRIEVLGIDVVPDDGEPGL
                     LRIEIDYRLRAA"
     gene            complement(354..1040)
                     /locus_tag="VGB85_29825"
     CDS             complement(354..1040)
                     /locus_tag="VGB85_29825"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEY0138335.1"
                     /translation="MSELDLHDDDDGGAYGPLLGLLFARVSNVDDPAKLGRVKVALPG
                     LSSTVESAWARVLAPGAGSKHGVYWPLEMNDEVVVGFVGGHPELPIVLGALWSATKLG
                     AVPEGSRRVHRLLTSASGHLLRMDDTEGAEKIELIAAKAENAITLDVGAGTVTIAART
                     RLEIKVGADITLTLADGKVSLACKEFSVSQCKDVTIASDAITLDGSQSLALNGTRSGV
                     NINNGALDIK"
     gene            complement(1037..2161)
                     /locus_tag="VGB85_29830"
     CDS             complement(1037..2161)
                     /locus_tag="VGB85_29830"
                     /inference="COORDINATES: protein motif:HMM:NF017739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HEY0138336.1"
                     /translation="MTLSTTNDSPELTVLAGARVLVRVDGNELTDAGSGVAVTRVEVR
                     HELGRPGSFVVHTADLTPEGIDWLDDFTAQEGGRVEISMGWGEETGRVFAGELVGVEL
                     EVTTPPASQVALRGHDRLHRLARARRTRAYVGKRDSEIAADLASEHGLELVGDATALV
                     HPYVMQADQTDLAFLRARARGIGHVFRVEDTRLLFGPRDLTGSPVATAELGKNLLELF
                     IRTTVLGQVGEIEARGWDPEDQKEVVASVAHARLRSRMGGQASGPARADEGFGAEVSS
                     AQGVAIAGKDAAVPGGTTPASPAEVAAAAAAAELESLALEHVRCEGSLLGDPSLRPGT
                     VLTIKGLGRRFSGDYWLTRVTHSFDDDGYRTGFEGRRTAT"
     gene            complement(2177..2944)
                     /locus_tag="VGB85_29835"
     CDS             complement(2177..2944)
                     /locus_tag="VGB85_29835"
                     /inference="COORDINATES: protein motif:HMM:NF039279.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY0138337.1"
                     /translation="MLMHDRGFPGLFGLGLASKLVIFHETGTKRLYNGSIAVPFNPSK
                     LSTSCAIDWQTITFGATTRQRAYAIQYTGDGASPATLSFDLNFDGYEGEPGSFTTGML
                     GTLSAPNPTAMHIFTPPSAVSVLPMVERIMQLQQITIELHRPPLCEVWWGQIRLIEGP
                     LTSLTQDFTRFLPDGTPVRANLGLTFTDASLTYTGELCSADVEKTYTVRLGDTLQAIS
                     ARQYGSPAHWRRIAEANAIDDPRRLVPGTILAIPALR"
     gene            complement(2964..4301)
                     /locus_tag="VGB85_29840"
     CDS             complement(2964..4301)
                     /locus_tag="VGB85_29840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138338.1"
                     /translation="MSTAWQQAVRPEIVRELLRPLLLARDVERGWLGVLLGRAAWPEL
                     LYPMLAAWNEQWSGSEDGSHGALPIVHARPPVHATTLETHVHHAAVVPRKTTTAPSLV
                     APPRVQARRASETSAPTTPAKRDAPAGDRLVLETRTITEAPPADRDPEPRRPPERPLP
                     RVVAAFRKDLSESAGRAPARREPAAAPAPAPPAASQPHTSAAPTRPTVEPRSPTPPPA
                     ARLQPVPGDMPPRVHPSAPAVTPSQGPVPGDIPLLHPPAAVAPSQQPVPGDIPLVHPT
                     APAVTPSQQPVPGDIPLVHARAPDLLASPTPARRIAAPTDPSISTPTPHPRVTARQPA
                     TPVPARTGARTAPPVPARWAEPSVTPPPRAPARSPLTSDLSHPRPVIPQPPQERPRLA
                     STPTPQPAPSVSPTPASQPPAPPPLDVESLIKTVEQRLAREFARARDLRRALR"
     gene            complement(4298..4837)
                     /locus_tag="VGB85_29845"
     CDS             complement(4298..4837)
                     /locus_tag="VGB85_29845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEY0138339.1"
                     /translation="MLGLNAIFALSSAALGRRLDPISNHHFLIEIDGLLTGGFSQVEG
                     LGGSIEVEPIHDGGSLGGARQQLGATTWDNIVLSHGVVEHDTLWNWYEATARGVIKRK
                     NGSIVLLDRQKAPVTLWNFRAGIPVVWRGPTLDAGASEVAVERIEIAHHGLEKPNSIR
                     ALQQVRAMVRYATGKEGLW"
     gene            complement(4849..5010)
                     /locus_tag="VGB85_29850"
     CDS             complement(4849..5010)
                     /locus_tag="VGB85_29850"
                     /inference="COORDINATES: protein motif:HMM:NF042798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6760 family protein"
                     /protein_id="HEY0138340.1"
                     /translation="MTGYPADRLHEEVAYLSMYLHWPYEQVMRLDHRERRRWVEQVAA
                     MNRERDAGP"
     gene            complement(5007..5387)
                     /locus_tag="VGB85_29855"
     CDS             complement(5007..5387)
                     /locus_tag="VGB85_29855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138341.1"
                     /translation="MVDAAHSSAPEFPFELPYGFVDGDNQRHRTGAMRLATAFDEIAP
                     LKDPRVQQNPGYILVILLSRVITRLGTLEYIHPKVIENLFASDLIYLQDMYVRLNRNG
                     RERMLVTCPHCEGDFEVEGPEPGK"
     gene            complement(5381..5818)
                     /locus_tag="VGB85_29860"
     CDS             complement(5381..5818)
                     /locus_tag="VGB85_29860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEY0138342.1"
                     /translation="MAKRRDPYGNYNFMVELDGLTRAGFKECAGLESSVDSFDYREGT
                     DPGNIARKLPGQRKASNITLKRGLTDDRALWDWHNKAASGNLERHTISIILRDETGEE
                     KMRWNIKNAWPTKWSGPNLDAGASEAAIESLELVHEGIEVQKW"
     gene            complement(5859..7016)
                     /locus_tag="VGB85_29865"
     CDS             complement(5859..7016)
                     /locus_tag="VGB85_29865"
                     /inference="COORDINATES: protein motif:HMM:NF016845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HEY0138343.1"
                     /translation="MEERAQRVPGVHREIVREAQDLVFRTGVPAFLGHAEGPTAPLLL
                     TRAAQFPGPLRLPDDSLLAPAIHGFFAAGGQRCFVLPVAPAAGHVGHLVAARQLGELE
                     EIDLLCAPDLLRPFTNKTSDAALAELVATQAQLIVECTAARCLMLVDGPPGRAQAQAH
                     ATALRARLDALGPGSLRDAALYFPWVRAPGAARFAPPCGHVAGTIARLDAQEGVHRAP
                     AGLELGGACDLGEPVDDGVQRQMTALGVNCIRAFRGRGLRIWGARTLSRDEAWQPIPV
                     RRLVSTIGRWLELRLADFAFESNDSMTWNRLDREVGAYLDGLHAAGALRGRVAAEAYF
                     IKCDAETNPPEVRDAGMLVAEIGVAPLVPQEFIALRLIQRGGGLSLTDAAG"
     gene            complement(7024..8196)
                     /locus_tag="VGB85_29870"
     CDS             complement(7024..8196)
                     /locus_tag="VGB85_29870"
                     /inference="COORDINATES: protein motif:HMM:NF016845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HEY0138344.1"
                     /translation="MPQYMSPGVYVEEVPSGTAPIAGVGTSTGGFVGLIPDGVTMPAM
                     PDGTRYTLAPAGEPQLVTNFTEFTTRFGEVSARVDANSVLGHAVRGYFVNGGTRLWVV
                     RVASLGAGESSDPIEDALAAMETVDEIALVAAPIKPPDGIDPIRLKAIYEKLLDHCER
                     LGDRFAILDSPEEVPVITADAINRLRESSYGAIYFPWIDVTGKGDYVPPSGHLAGMYG
                     RVDATRGVHKAPANEVLRGALGLKYRVGKPIQDDLNPRGVNALRVFGNSIKVWGARTL
                     KPGDDFTYINIRRLFNFLRESIDEGLQWAVFEPNTSDLWAKVTRNVSAFLTNVWSSGA
                     LFGNTPEQAFYVKCDDETNPPIGRDLGMVVTEIGVAVVKPAEFVVFRLSQRSTTGK"
     gene            complement(8221..8790)
                     /locus_tag="VGB85_29875"
     CDS             complement(8221..8790)
                     /locus_tag="VGB85_29875"
                     /inference="COORDINATES: protein motif:HMM:NF025431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HEY0138345.1"
                     /translation="MTVLLRQELPEDLRAQIGISFATPNDDFPPAGVALPALNLFLYD
                     IMTSPDLRHSGAGWEQQAPGQFLRTPDPYRIACHYIVTAWAREGTPAPEEDEHRLLGE
                     AMRALLRYRILPPHAVVGEFARIGIPIHVRALEGGRLGSPGEFWQALGGKPRPFFNYS
                     LIFALPVSDPEVSTAAVTETRVEIGRKQP"
     gene            complement(8998..>9561)
                     /locus_tag="VGB85_29880"
     CDS             complement(8998..>9561)
                     /locus_tag="VGB85_29880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138346.1"
                     /translation="PPPVPTVAPSRRVPLPGIIAGIVALLAVVVIIVTLTASPPPPPE
                     PTPTSEPAPARTIEVRPESAPEPAPEPPAPIVTPEPPPALPAVTPTPEKNKTRPSKPT
                     TSPTSTGVCPSELTGYLENGLPAPAGGGARPDLRVRVAADGKLSYESLGGADDYPAQA
                     RARLPRLSDRLQAKHAAALPCSMKLLR"
BASE COUNT         1275 a         3027 c         3784 g         1475 t
ORIGIN      
        1 ctggcggcgc gcaggcggta gtcgatctcg atgcgcagca ggccgggctc accgtcgtcg
       61 ggcacgacgt cgatgccgag cacctcgatg cgcggctcga agcgggtcag ggcgccgtgc
      121 acggccccgt ggatccgccc gatcgtggtc gggttggcgg cggcgaacac gaggtcatgg
      181 atcgcgcagc cgaactcggg ctgcatcatg cgctcgcccg gggcggtctc gaggatcgcc
      241 cagatcgcct gctcgacgca ggcctcgtcg ctgaccgtct gcaggcgacc ggccacgaac
      301 gtcagcggcg cctgccaacc tgtcccgaga aacttccagg ccatcgctgt ccctcacttg
      361 atgtcgagcg cgccgttgtt gatgttgacg ccgctgcggg tgccgttgag cgcgaggctc
      421 tggctgccgt cgagcgtgat cgcgtcgctg gcgatcgtga cgtccttgca ctgcgagacc
      481 gagaattcct tgcaggcgag cgagaccttg ccgtcggcga gcgtcagggt gatgtcggcg
      541 ccgaccttga tctcgagcct ggtcctggcc gcgatcgtca cggtgccggc gccgacgtcg
      601 agggtgatcg cgttctccgc cttggcggcg atcagctcga tcttctcggc gccctcggtg
      661 tcgtccatgc gcagcaggtg gccgctcgcg gaggtcagca ggcgatgcac gcgacgcgag
      721 ccctccggca cggcgccgag cttcgtcgcg ctccacaggg ccccgagcac gatcggcagc
      781 tcggggtggc cgccgacgaa gccgaccacg acctcgtcgt tcatctcgag cggccagtag
      841 acgccgtgct tgctgcccgc gccgggcgcg agcacccgcg cccacgccga ctcgacggtc
      901 gacgacagac ccggcaaggc gaccttgacg cgaccgagct tcgccgggtc gtcgacgttg
      961 ctgacgcgcg cgaacagcag gccgagcagg gggccgtagg cgccgccatc gtcgtcgtcg
     1021 tggaggtcga gctcgctcat gtggcagtgc gcctcccctc gaagccggtg cgatagccgt
     1081 cgtcgtcgaa gctgtgggtc acgcgggtca gccagtagtc gccggagaag cggcggccga
     1141 ggcccttgat cgtcagcacg gtgccggggc gcaggctggg gtcgccgagc aggctgccct
     1201 cgcagcgcac gtgctcgagc gcgaggctct cgagctcggc ggccgcggcc gcggcggcga
     1261 cctcggccgg agacgcgggg gtggtgccac cgggcacggc ggcgtccttg ccggcgatcg
     1321 caacgccctg cgcgctcgag acctcggcgc cgaagccctc gtcggcgcgc gccgggccgc
     1381 tggcctgacc gcccatccgg gagcgcaggc gggcgtgcgc gacgctggcg acgacctctt
     1441 tctgatcctc ggggtcccag ccgcgcgctt cgatctcgcc gacctggccg agcacggtgg
     1501 tgcggatgaa cagctcgagc aggttcttgc cgagctcggc ggtggcgacg gggctgccgg
     1561 tgaggtcgcg cgggccgaac agcaggcggg tgtcctcgac gcggaacacg tggccgatgc
     1621 cgcgggcccg cgcgcgcagg aaggcgaggt cggtctggtc ggcctgcatg acgtaggggt
     1681 ggaccagcgc ggtcgcgtcg ccgacgagct cgaggccgtg ctcgctggcg aggtcggccg
     1741 cgatctcgct gtcgcgcttg ccgacgtagg cccgggtccg acgcgcgcgc gcgagccggt
     1801 gcaggcgatc gtggccgcgt agcgccacct gcgaggccgg cggggtggtg acctcgagct
     1861 cgacgccgac gagctcaccg gcgaacacgc gtccggtctc ttcgccccag cccatcgaga
     1921 tctcgacgcg cccgccctcc tgcgcggtga agtcgtcgag ccagtcgatg ccctcgggcg
     1981 tgaggtcggc ggtgtgcacg acgaagctgc ccggccgacc gagctcgtga cggacctcga
     2041 ccctggtcac cgcgacgccg ctgcccgcgt cggtcagctc gttgccgtcg acgcgcacga
     2101 gcacgcgcgc gcccgcgagg acggtcagct ccggtgaatc gttggtggtc gagagagtca
     2161 tgagcgaagc gccgggctag cgaagcgccg gaatagcaag gatggttccg gggacgaggc
     2221 ggcgggggtc gtcgatcgcg ttggcctcgg cgatccgccg ccagtgcgcc gggctgccgt
     2281 actgacgcgc cgagatcgcc tgcagcgtgt cgccgaggcg cacggtgtag gtcttctcga
     2341 cgtcggcgga gcacagctcg ccggtgtacg tcagcgaggc atccgtaaaa gtgaggccga
     2401 gattggcgcg cacgggcgtg ccgtcgggca ggaagcgggt gaagtcctgg gtgaggctgg
     2461 tcagcggacc ctcgatcagg cggatctggc cccaccacac ctcgcacagc ggcgggcggt
     2521 gcagctcgat cgtgatctgt tgcagctgca tgatccgctc gaccatcggc agcacgctga
     2581 cggcgctcgg cggggtgaag atgtgcatcg ccgtcgggtt cggggcgctg agcgtgccga
     2641 gcatgccggt cgtgaacgat ccgggctcac cctcgtagcc gtcgaagttg aggtcgaagg
     2701 acagcgtcgc cgggctcgcg ccgtcgccgg tgtactggat cgcgtaggcc cgctgccgcg
     2761 tggtcgcgcc gaaggtgatc gtctgccagt cgatcgcgca cgaggtcgag agcttgctcg
     2821 ggttgaacgg gaccgcgatg ctgccgttgt agaggcgctt ggtcccggtc tcgtgaaaga
     2881 tgaccagctt cgacgccagc ccgaggccga acaggcccgg gaagccgcgg tcgtgcatca
     2941 gcatggtggg gtcctcctcg agctcatcgc agggccctcc gcaggtcgcg ggcgcgcgcg
     3001 aactcgcggg cgagccgctg ctcgaccgtc ttgatgagag actcgacgtc cagcggcggg
     3061 ggcgcgggtg gctgggacgc gggcgtcggg ctgaccgaag gcgctggttg tggcgtgggg
     3121 gtgctggcga ggcgggggcg ctcctgcggc ggctggggga tgacgggacg cgggtggctc
     3181 aggtcgctgg tgagcggtga gcgggcgggg gcgcggggtg gcggggtgac gctcggttcg
     3241 gcccagcggg ctggcaccgg tggggcagtg cgcgcgccgg tacgggcggg cacgggcgtg
     3301 gcgggctggc gcgcggtgac gcgggggtgt ggggtcggcg tggaaatgga ggggtccgtg
     3361 ggcgcggcga tccggcgcgc gggcgtcggg gaggcgagga ggtcgggcgc tcgggcgtgg
     3421 acgagcggga tgtccccggg gacaggttgc tgcgacgggg tcacggctgg agccgtggga
     3481 tggacgagtg ggatgtcccc ggggacaggt tgctgcgacg gggccacggc ggcagggggg
     3541 tggaggagcg ggatgtcccc ggggacaggt ccctgcgacg gagtcacggc tggcgccgag
     3601 gggtggacgc gagggggcat gtccccgggg acaggttgca gcctcgccgc ggggggaggg
     3661 gtgggcgagc gtggctcgac ggtcgggcgt gtcggagcgg cggaggtgtg gggctgcgat
     3721 gccgcggggg gcgcgggggc cggggctgcg gcgggctcgc ggcgtgcggg cgcacggcct
     3781 gcgctttcgg acaggtcctt gcgaaacgcc gcgaccacgc gtggcagcgg gcgctcgggg
     3841 gggcgtcggg gttcggggtc tcggtccgcg ggtggggctt cggtgatggt gcgggtctcg
     3901 aggaccaggc gatcgcctgc aggtgcgtcg cgcttcgcag gggtggtggg agcggatgtt
     3961 tcgctggcgc gtcgcgcttg cacccgcggc ggcgcgacga gggacggcgc ggtggtcgtc
     4021 ttgcgcggca cgaccgcggc gtggtgcaca tgggtctcga gcgtcgtcgc gtggaccggc
     4081 gggcgcgcgt gcacgatcgg gagagcgccg tgggagccgt cctcgctgcc gctccactgc
     4141 tcgttccagg cggcgagcat cggatagagc agctcgggcc aggcggcgcg gccgagcagc
     4201 acgccgagcc agccgcgctc gacgtctcgc gcgagcagga gcggccgcag cagctcgcgc
     4261 acgatctcgg gtcgcacggc ctgttgccac gcggtgctca ccacaggccc tccttgccgg
     4321 tcgcgtagcg gaccatcgcg cgcacttgct ggagcgcccg gatcgagttg ggcttctcga
     4381 ggccgtggtg ggcgatctcg atgcgctcga cggcgacctc gctggcgccg gcgtcgaggg
     4441 tcgggccgcg ccacacgacg gggatgccgg cgcggaagtt ccacagggtg acgggggcct
     4501 tctggcggtc gagcaggacg atcgacccgt tcttgcgctt gatgacgccg cgggcggtcg
     4561 cctcgtacca gttccacagg gtgtcgtgct cgacgacgcc gtgcgacagc acgatgttgt
     4621 cccacgtggt ggcgccgagc tgctggcgcg cgccgccgag gctgccgccg tcgtggatcg
     4681 gctcgacctc gatgctgccg ccgaggccct cgacctgcga gaagccgccg gtcagcaggc
     4741 cgtcgatctc gatcaggaag tggtgattgg agatcgggtc gaggcgacgg ccgagggccg
     4801 cgctgctgag ggcgaagatg gcgttgagtc cgagcatgat gtcttcttct acgggccggc
     4861 gtcgcgctcg cggttcatcg ctgcgacctg ctcgacccag cgccgccgct cgcggtggtc
     4921 gaggcgcatg acctgctcgt agggccagtg caggtacatc gagagatagg ctacctcctc
     4981 gtgcaggcgg tccgcggggt agccggtcat tttcccggtt cggggccctc cacctcgaag
     5041 tcgccctcgc aatgtgggca ggtgacgagc atgcgctcgc gcccgttgcg attgaggcgg
     5101 acgtacatgt cctggagata gatgaggtcg gaggcgaaca ggttctcgat caccttgggg
     5161 tggatgtact cgagggtgcc gaggcgcgtg atgacgcgac tgagcaagat gacgaggata
     5221 tagccggggt tctgctggac gcgggggtcc ttgagcgggg cgatctcgtc gaaggcggtg
     5281 gcgaggcgca tcgcgccggt gcgatggcgt tgattatcgc cgtcgacgaa gccgtagggc
     5341 agctcgaacg ggaactctgg cgcgctgctg tgcgcggcgt ctaccatttt tggacctcga
     5401 taccctcgtg gaccagctcg agcgactcga tcgcggcctc ggaggcgccg gcgtcgaggt
     5461 tgggtccgct ccacttggtc ggccacgcgt tcttgatgtt ccagcgcatc ttctcctcgc
     5521 cggtctcatc gcgcaggatg atcgagatcg tgtggcgctc gaggttcccg gaggcggcct
     5581 tgttgtgcca gtcccacagc gcgcggtcgt cggtcaggcc gcgcttgagg gtgatgttgc
     5641 tcgccttgcg ctgcccgggc agcttgcgcg cgatgttgcc ggggtcggtg ccctcgcggt
     5701 aatcgaagga gtcgaccgag gactcgagcc ccgcgcactc cttgaagccg gcccgcgtga
     5761 gaccgtcgag ctcgaccatg aagttgtagt ttccgtaggg atctctgcgc ttggccattg
     5821 tctgggtctc ctgcgatgct gtcgaagcgt cgaagcggtc agccggcggc gtccgtgagg
     5881 ctgaggcccc cgccgcgctg gatcaggcgg agggcgatga actcctgggg tacgagcggc
     5941 gcgacgccga tctcggcgac cagcatcccg gcgtcacgga cctcgggcgg gttggtctcg
     6001 gcgtcgcatt tgataaagta ggcctcggcg gcgacgcggc cgcgcagagc cccggcggcg
     6061 tgcagcccgt cgaggtaagc gccgacctcg cggtcgaggc ggttccaggt catcgagtcg
     6121 ttggactcga aggcgaagtc ggcgaggcgc agctcgagcc agcggccgat cgtcgagacg
     6181 aggcggcgca cggggatcgg ctgccaggcc tcgtcgcggc tcagcgtgcg cgcgccccag
     6241 atccgcaggc cgcggccacg aaaggcgcgg atgcagttga cgccgagcgc ggtcatttgt
     6301 cgttgcacgc cgtcgtcgac gggctcgccg agatcacagg cgcccccgag ctcgaggccg
     6361 gcgggcgcgc ggtggacccc ctcctgcgcg tcgaggcggg cgatcgtgcc tgcgacgtgg
     6421 ccacacggcg gggcgaagcg cgcggccccg ggcgcgcgca cccacgggaa gtacagggcc
     6481 gcgtcgcgca gcgagccggg gccaagagcg tcgaggcggg cgcgcagggc ggtcgcgtgg
     6541 gcctgcgcct gcgcgcggcc gggcgggccg tcgacgagca tcaggcagcg ggccgcggtg
     6601 cattcgacga tcagctgcgc ctgggtggcg accagctcgg cgagcgcggc gtcgctggtc
     6661 ttgttggtga agggtcgcag caggtccggc gcgcacagga ggtcgatctc ctcgagctcg
     6721 ccgagctggc gcgcggccac gagatggccg acgtgaccgg ccgcgggcgc cacgggcaac
     6781 acgaagcagc gctggccgcc ggcggcgaag aacccgtgga tcgcaggcgc gagcaggctg
     6841 tcgtcgggga ggcgcagcgg gcccgggaac tgggccgcgc gggtcagcag cagcggggcc
     6901 gtcgggccct cggcgtgacc gaggaacgcg ggcacgcctg tgcgaaagac gaggtcctgc
     6961 gcctcgcgca cgatctcccg gtgtacgccg gggacccgct gtgcccgctc ctccatcgtc
     7021 tgcttacttc cccgtggtcg agcgctggct gaggcggaag acgacgaact cggccggctt
     7081 gacgaccgcg acgccgatct cggtgacgac catgccgagg tcacggccga tcggcgggtt
     7141 ggtctcgtcg tcgcacttga cgtagaaggc ctgctccggc gtgttgccga acagcgcgcc
     7201 ggagctccac acgttggtca ggaaggcgct gacgttgcgc gtgaccttgg cccacaggtc
     7261 ggaggtgttg ggctcgaaga cggcccactg caggccctcg tcgatcgact cgcgcaggaa
     7321 attgaacagg cggcggatgt tgatatacgt gaagtcgtcg ccgggcttga gcgtgcgggc
     7381 gccccacact ttgatagagt tgccgaacac gcgcagcgcg ttgacgccgc gcgggttgag
     7441 gtcgtcctgg atcggcttgc cgacgcggta cttgaggccg agcgcgccgc gcagcacctc
     7501 gttggcgggg gccttgtgca cgccgcgggt ggcatcgacg cggccgtaca tgccggcgag
     7561 gtggccgctg ggcggcacgt agtcaccctt gccggtgacg tcgatccacg ggaagtagat
     7621 cgcgccgtag ctgctctcgc gcaggcggtt gatcgcgtcg gcggtgatca ccgggacctc
     7681 ctcgggcgag tcgaggatcg cgaagcggtc gccgaggcgc tcgcagtggt cgagcagctt
     7741 ctcgtagatc gccttgaggc ggatcgggtc gatgccgtcg ggcggcttga tcggcgcggc
     7801 gacgagcgcg atctcgtcga cggtctccat cgccgcgagg gcgtcctcga tcgggtcgct
     7861 cgactcgccg gcgcccagcg aggcgacccg cacgacccac aggcgggtgc cgccgttgac
     7921 gaagtagccg cgcaccgcgt ggccgagcac gctgttggcg tcgaccctgg ccgagacctc
     7981 gccgaagcgg gtggtgaact cggtgaagtt ggtgacgagc tgcggctcgc cggcgggggc
     8041 cagcgtatag cgggtgccgt cgggcatcgc aggcatcgtg acgccgtcgg ggatcaggcc
     8101 gacaaagccg ccggtgctgg tgccgacgcc ggcgatcggc gcggtcccgg acgggacctc
     8161 ctcgacgtat acccctgggg acatgtactg cggcattcgt ggctctcctg tctgaggctc
     8221 tcagggctgc ttgcgcccga tctcgacgcg cgtctcggtg acagccgccg tggacacttc
     8281 ggggtcgctg accggcagcg cgaagatcag cgagtaattg aagaagggcc tgggcttgcc
     8341 gcccagcgcc tgccaaaatt cgcccgggct gccgaggcga ccgccctcga gcgcacggac
     8401 gtgaatgggg atgccgatcc gcgcgaactc gccgacgacg gcgtgcggcg gcaagatgcg
     8461 atatcgcagc agcgcgcgca tcgcctcgcc gagcaggcgg tgctcgtcct cctcgggcgc
     8521 gggcgtgccc tcgcgggccc acgctgtcac gatgtaatga caggcgatgc gatacgggtc
     8581 cggcgtgcgc aggaactggc ccggcgcctg ctgctcccag ccggcgccgc tgtggcgcag
     8641 gtccgggctg gtcatgatgt cgtagaggaa gagattgagg gccggcagag cgacgccggc
     8701 gggcgggaag tcgtcgttcg gcgtggcgaa cgatatccct atctgtgcac gtaggtcctc
     8761 gggtaattcc tggcgcagga gtaccgtcaa tccctggtga agctcatgca acatctaacc
     8821 acgcgggaga ttttcatggt ttctcgtggc cggtcaactc gttattttcg agcgatagtg
     8881 cgataaagag gcggttctgc gcgctggcgg aagtgcggag ataactgtgc agatatcgcc
     8941 gacgtcgtcg gcgtaatctg ccgggtgctg cggtccacga tcgtcgacgc ggacggatca
     9001 gcgcaatagc ttcatgctgc acgggagcgc ggcggcgtgc ttcgcctgca agcggtcgct
     9061 caggcgcggc aacctggcgc gcgcctgcgc ggggtagtcg tcggcgccgc cgagcgactc
     9121 gtacgagagc ttgccgtcgg ccgcgacgcg gacgcggaga tcggggcgcg ctcctccacc
     9181 cgcgggcgcg ggcaggccgt tctcgaggta gccggtgagc tcggagggac atacgccggt
     9241 cgaggtcggc gaggtcgtgg gcttgctcgg gcgggtcttg ttcttctcgg gtgtcggtgt
     9301 gacggcgggg agcgcgggcg gcggctcggg ggtgacgatc ggcgcaggcg gctcgggcgc
     9361 gggttcgggt gctgactcgg gtcgtacttc gatggttcgt gcgggcgcgg gctcgctcgt
     9421 cggcgtgggt tcggggggcg gtggcggcga cgcggtgagc gtgacgatga tgacgacgac
     9481 ggcgagaagc gcgacgatcc cggcgatgat gccgggcagc gggacgcggc gcgagggcgc
     9541 cacggtcggc acgggtggcg g
//
LOCUS       DASWNJ010001785         4441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_279974, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001785 DASWNJ010000000
VERSION     DASWNJ010001785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279974"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VGB85_29885"
     CDS             complement(<1..522)
                     /locus_tag="VGB85_29885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138347.1"
                     /translation="MALSRRRLLAGTASLALPACADAGPPQTRVFHAPPLLVRSPAPW
                     KHDLGELDDVDPALRPAFTDARGFALLPPPARQGWRTLRPEPPQTIAGFIDSAPNTRT
                     APRDRIALLPLGEFPLEVVVDGKFIGIVHTPELPAIAAVLAAFFATPVDILPCEPLPT
                     DGLARRVQWDRSQF"
     gene            complement(572..2686)
                     /locus_tag="VGB85_29890"
     CDS             complement(572..2686)
                     /locus_tag="VGB85_29890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEY0138348.1"
                     /translation="MHPPASLASWTRGALQLGLLLGVGLAFVGAGPAPLAAAPMPCQH
                     ETPCTFKKPLLLFVVEYSGAMNQPHDADSTRWQAAVETIGDTIDFDNGYIAEAFILGL
                     LRYGHDPAPAVANTPIVGDTTGLLDGHALDVPWYDPADPDKAYVECIAGDAIVAALAD
                     LPAPADGIAGWTRGALDFTQTYLAQVDADHPEDEGQRRATIVLITTGTWTDPTGTMQL
                     GPPEQDPELTAATLYDEQKVATHVINVGGPMGALLSAPVAAAGGGEVFAAEDFVGVSD
                     ALKLFIDDIQNDVPAPVCTPAVPRVMVLLDASSSMLNVGDMQAGPGQGAWDQARDALA
                     GDDSVFDIFFDAASVESAYHAGLAVFGGAAPAEQQILVQYGPCHKDMFAWALDPASSC
                     VAPGCTDPYAGPPITWTFKNGADAMPPFPDDVFSHMPRCDKSDAKPQACAGSGSYVHL
                     GLELVADNIEAYRAQCLAPNAAHPCDDTTPFFNVLITDGAYDSTDAQVQAPLVAMAAA
                     GVVTRVIGLGPASDLAQLEAMADWGSGSVLDPILADSQSQLADALASLFVQPTLGPCC
                     SFTDCNFPDDSFGEDDDGGDGSLDGSDTEVDDTGATGPVTTTAPDDTSTTGAPETTSA
                     TTDLTTSPTTAEPDPTATPTTGPPDPSNLTNTTPGADSDVLPPVGSPDDGCGCDTRGR
                     GPAHLLVLVALAGLRRRRATAR"
     gene            2981..>4441
                     /locus_tag="VGB85_29895"
     CDS             2981..>4441
                     /locus_tag="VGB85_29895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0138349.1"
                     /translation="MSVPAKARVLLVGGAADPAALDDALAGLDVGLVQVGADEDAPGD
                     ELAAIVLAGDADGLALARRLRSRHPATPLLFVVDELAGWPLAQAYALGAVDHVTRPFV
                     SQVVRSKLAFLVEHFRRLGASDDDDARERARAEAELRASEQRLRLATEAAGLGIWVWH
                     LADNRVTWENDRLYEIFGLLRSDEPVDAIHFMADFVHPDDAASFERAMAATLKSGAKF
                     YFLGRFRRKDGELRWTELTGKLVTDAEGTPVHVLGTAADITDDKRAEDEIRRVAAESA
                     AVAEANAKFRVFFEQGTQYAGVMTLDGTVVEANRLCLEACGFRREEVVGKPFWECGWW
                     NRSPALMETIREASMQAAEGRLFRTEIHYFVADGSQRFVDLTIAPVTGDDGRVLFVAP
                     TGTDITERRRAERALRASEERLRFLDALAEATRAAADPGDIMATTARLLGQHLAVTRT
                     AYADVDADSDRFTIRNDWTTTGASSAAGVYSLDLFGP"
BASE COUNT          601 a         1452 c         1761 g          627 t
ORIGIN      
        1 gaattgcgag cggtcccact ggacccgccg cgcgagcccg tcggtcggca gtggttcaca
       61 gggcaggatg tcgaccggcg tggcgaagaa ggccgcgagc accgctgcga tcgcgggcag
      121 ctccggggtg tggacgatgc cgatgaactt gccgtcgacg accacctcga gcggaaattc
      181 gccgagcggc agcagggcga tgcggtcgcg cggcgcggtg cgggtgttcg gggccgagtc
      241 gatgaacccg gcgatcgtct gcggtggttc gggtcgcagc gtccgccagc cctggcgcgc
      301 aggtgggggc agcagggcga agccgcgcgc gtcggtgaac gcggggcgca gcgcggggtc
      361 gacgtcgtcg agctcgccga ggtcgtgctt ccacggcgcg ggcgagcgga ccagcagcgg
      421 gggcgcgtga aagacccgcg tctgcggggg cccggcgtcc gcgcaggcag gcagcgcgag
      481 ggaggccgtg ccggcgagca ggcgacgtcg ggagagcgcc atcgcggggg tcgaacggac
      541 gacgggcggc cgcggttcgt cccgccgaaa ttcatcgggc cgtggcacgc cggcggcgaa
      601 ggccggcgag cgccacgagc accaacagat gcgcgggccc gcgaccacgg gtgtcgcacc
      661 cacaaccatc gtcgggcgag ccgaccggcg gcagcacgtc gctgtccgct cccggcgtgg
      721 tgttggtcag gttcgaggga tcgggcgggc cggtggtcgg cgtggcggtc gggtcgggct
      781 ctgcggtcgt ggggctcgtg gtgaggtccg tggtcgcgct cgtcgtctcc ggggcgcccg
      841 tcgtgctcgt gtcgtccggt gcggtcgttg tgaccggccc ggtggcgccg gtgtcgtcga
      901 cctcggtgtc ggagccgtcg agcgagccat cgccgccatc atcgtcctcg ccgaacgagt
      961 cgtccgggaa gttgcagtca gtgaacgagc agcacgggcc gagggtgggc tgcacgaaca
     1021 ggctcgcgag cgcgtcggcg agctgactct gactgtcggc gaggatcggg tcgaggacgc
     1081 tacccgagcc ccagtccgcc atcgcctcga gctgcgcgag gtcactcgcg ggaccgaggc
     1141 cgatcacccg cgtgacgacg ccggccgcgg ccatcgccac cagcggggcc tggacctgcg
     1201 cgtcggtcga gtcgtacgcg ccgtccgtga tcaggacgtt gaagaagggg gtcgtatcgt
     1261 cgcacgggtg cgcggcgttc ggcgcgaggc actgggcgcg ataggcctcg atgttgtcgg
     1321 cgaccagctc gagaccgagg tgcacgtagc tgccggagcc ggcgcaggcc tgcggtttgg
     1381 cgtcactctt gtcgcagcgc ggcatgtgac tgaagacatc gtccgggaat ggcggcatcg
     1441 cgtcggcgcc atttttgaaa gtccaggtga tcggtgggcc ggcgtagggg tccgtgcagc
     1501 cgggggcgac gcacgagctc gcggggtcga gggcccaggc gaacatgtcc ttgtggcaag
     1561 gtccatattg cacgaggatc tgctgctcgg ccggggctgc gccgccgaac accgccaggc
     1621 cggcgtggta ggccgactcg acgctcgcgg cgtcgaagaa gatgtcgaac accgagtcgt
     1681 cgcccgcgag cgcgtcgcgg gcctggtccc acgcgccttg accggggccg gcctgcatgt
     1741 cgccgacgtt gagcatcgac gacgaggcgt cgagcagcac catcacgcgc gggacggccg
     1801 gcgtgcagac cggcgcgggg acatcgttct ggatgtcgtc gatgaagagc ttgagggcat
     1861 cggagacgcc gacgaagtcc tcggccgcga agacctcgcc gccgccggcc gccgcgacgg
     1921 gggcgctgag cagcgcgccc atgggcccgc cgacgttgat cacgtgggtg gcgacctttt
     1981 gctcgtcgta tagcgtcgcg gcggtgagct cggggtcttg ctcgggcggg ccgagctgca
     2041 tggtgccggt cgggtcggtc caggtcccgg tggtgatcag cacgatcgtc gcgcggcgct
     2101 ggccctcgtc ctcgggatga tcggcgtcga cctgcgcgag gtaggtctgc gtgaagtcga
     2161 gggcgccccg ggtccagccg gcgatgccgt cggcgggcgc gggcaggtcc gcgagcgccg
     2221 cgacgatcgc gtcgccggcg atgcactcga cgtacgcctt gtcggggtcc gccgggtcgt
     2281 accagggcac atcgagcgcg tggccgtcga gcaggccggt ggtgtcaccg acgatcggtg
     2341 tgttagcgac ggccggcgcg gggtcgtggc cgtagcggag caggccgagg atgaaggcct
     2401 ccgcgatgta gccgttgtcg aagtcgatgg tgtcgccgat cgtctcgacg gcggcctgcc
     2461 agcgcgtcga gtcggcgtcg tggggctggt tcatcgcgcc cgaatactcg acgacgaaca
     2521 gcagcagcgg cttcttgaac gtgcacggtg tctcgtgctg gcacggcatc ggggccgcgg
     2581 cgagcggggc cggcccggcg ccgacgaacg cgaggccgac gcccagcagc aggccgagct
     2641 ggagggcgcc acgtgtccac gaggcgaggg acgcgggcgg gtgcatcgcg gcattgtctg
     2701 cttttgtgaa tgaaacggcc accgacccgg gagatccacg ggaacgcggc gtgacgggcc
     2761 gaatacgcgg gttcgaggtg ctcggcgtgg acatcgccgg gcgcgtgcgg gcccccgggc
     2821 ggttgctcgc ggggggtcgg attgataccg tggcggcggc ttttatcgca ggtccgcccg
     2881 tgccactcgt ggacaacctc ccatcgccgg gtcccgatcg tggtgcgcgg gccgaagacc
     2941 tgtccccggg gacatgtggg cacggactgt ggggccgacg ttgagcgtac cggcgaaggc
     3001 ccgtgttctg ctcgtcggcg gggcggcgga tccggccgcg ctggatgacg cgctcgccgg
     3061 gctggatgtc gggctcgtgc aggtcggcgc cgacgaggac gcgccgggcg acgagctcgc
     3121 cgcgatcgtg ctcgcgggcg acgcggacgg gctcgcgctc gcgcgacggc tgcgctcgcg
     3181 gcacccggcg acgccgctgt tgttcgtggt cgacgagctc gccgggtggc cgctggcgca
     3241 ggcgtacgcg ctcggcgcgg tcgatcacgt gacgcgacct tttgtctcgc aggtcgtgcg
     3301 cagcaagctc gcgttcctcg tcgagcactt ccggcggctg ggcgcgagcg acgatgacga
     3361 cgcgagggag cgggcgcggg cggaggcgga gctgcgggcg agcgagcagc ggctgcgcct
     3421 ggcgaccgag gcggcggggc tgggcatctg ggtgtggcac ctcgccgaca accgcgtcac
     3481 ctgggagaac gaccgcctct acgagatctt cgggctgctg cgcagcgacg agccggtcga
     3541 cgcgatccac ttcatggcgg acttcgtgca tcccgacgac gccgcgtcgt tcgagcgggc
     3601 gatggccgcg acgctcaagt ccggggcgaa gttctacttc ctcggccggt ttcgccgcaa
     3661 ggacggcgag ctgcgctgga ccgagctcac cggcaagctg gtgaccgacg ccgagggcac
     3721 gccggtgcac gtgctcggca ccgcggccga catcaccgac gacaagcgcg ccgaggacga
     3781 gatccggcgc gtggcggccg agtcggcggc ggtcgccgag gccaacgcga agtttcgcgt
     3841 gttcttcgag cagggcacgc agtacgccgg cgtgatgacg ctcgacggca ccgtcgtcga
     3901 ggccaaccgg ctgtgcctcg aggcctgcgg gtttcgccgc gaggaggtcg tcggcaagcc
     3961 gttctgggag tgcggctggt ggaaccgatc gccggcgctg atggagacga tccgcgaggc
     4021 gtcgatgcag gcggccgagg gccggctgtt ccgcaccgag atccactact tcgtcgccga
     4081 cggcagccag cgcttcgtcg acctgacgat cgcgcccgtc accggcgacg acggtcgcgt
     4141 gctgttcgtc gcgccgaccg gcaccgacat caccgagcgc aggcgggccg agcgggcgct
     4201 gcgggccagc gaggagcggc tgcgcttcct cgacgcgctc gccgaggcga cccgcgccgc
     4261 ggccgacccc ggcgacatca tggcgacgac cgcccggctg ctcgggcagc acctcgccgt
     4321 cacgcgcacg gcctacgcgg acgtcgacgc cgacagcgac cgcttcacga tccgcaacga
     4381 ctggaccacc acgggggcga gcagcgcggc cggcgtgtac tcgctcgacc tgttcgggcc
     4441 g
//
LOCUS       DASWNJ010001786         2345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_67313, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001786 DASWNJ010000000
VERSION     DASWNJ010001786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67313"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            626..>2345
                     /locus_tag="VGB85_29900"
     CDS             626..>2345
                     /locus_tag="VGB85_29900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138350.1"
                     /translation="MTLGVPAASAAAATTGGATKCENPVTVRSEGRANGTVCPDQLAS
                     RGLTVIDLADTWVPYALSGAAIDAGQTPPAYRETYVELAAEKVPPGTITADDGAFEFF
                     GVPPNLHLVLARMDDEARHRCHDGVADESLTGLARTLRWVHRDQLEVDRKRLARDRGL
                     VQAAMRKRKLASPDALAAASKPHAKLIANLKVAEQQVAALTAVQDHLVCDGLLATKDL
                     ARGQFDHATALALKQYQLRHWIVGSGELDGETMTSLREDSRELDFRLALRVLRQRVSD
                     AAGIIEDGSARAEWAPVLGRHLDGSALRYRGAHEPMANGAPDLVSPATEAAARALGWQ
                     DYATARASLRQLLSGSESLRVAVQLPPVPSYHSKTMELRAVIDPGEVSYDVPKQRGAS
                     RAGPKAERRPVLIVYAKDGDKEVALVRWPTTIGGWQDEKLANGAIVRRYKPSDTGPRL
                     WRDLVVAPVWYPPKSTPDNELVRARNGKWELKEDLIGPGYRSAYGLAMLIHHMVVSKP
                     GGDVLYDRAIRTHGSAHYQSITRADSHGCHRTYNHQILGLASFLLHHHAYTVKGPLQE
                     KYVRKVR"
BASE COUNT          348 a          851 c          836 g          310 t
ORIGIN      
        1 gacgtgctgc tggcgatctg gctcgcggcg atctacgggc cgacccccgc acgatgaggc
       61 cgtgaggctc acggcggggg cgcgcagccc atgccgtact cgcagtaacc gtcacacacc
      121 tcgatcatcg tctcctgcat gcagccgtcg tcgagcatgc gcgtccgcgc cagaaggtcg
      181 tgctctgctg gcgtgggacc gggcccgggc gcgaagacgt cggcgcctgc gtggtccgcc
      241 gccggggcgc ccgggaagct ccgggtcgcg gcgttcgtgg gcccgtcgca tcccgcgaat
      301 tcgtcgagct cgcacaaata cgaagatgcc gatcctgaca tcgacgggcg gccggaccgc
      361 actgtgtcgc ggggccccca cgtgactccg cggcctcgct gcgccagatc ggcgcaggtc
      421 ggcctccggg acgccgctgc ggcccgatcc cgcgaatgcg tggtggtcgt cgaggcgcgg
      481 ctgcgtgcta catcgaatgc aaacaccaat gtctcacgca cggtcccacg cacgcgcaac
      541 agctccacgg tcccccgcgt cgaacgccag cgccacgcgc ataagccggc gcgttcgcct
      601 cgatatgttc gtattgctgg ccggtgtgac gctcggcgtc ccggccgcga gcgccgccgc
      661 cgcgaccacg ggtggggcga cgaagtgcga aaatccggtc accgtccgca gcgagggacg
      721 ggcgaacggg acggtatgtc cggaccagct ggcgtcgcgc ggcctgacgg tgatcgacct
      781 cgccgacacc tgggtgccct acgcgctctc cggcgcggcg atcgacgccg gccagacgcc
      841 gccggcctac cgcgagacct acgtcgagct cgccgccgag aaggtcccgc cgggcacgat
      901 caccgcggac gacggcgcgt tcgagttctt cggcgtgccg ccgaacctgc acctcgtgct
      961 cgcgcgcatg gacgacgagg cccgccatcg ctgtcacgac ggcgtcgccg acgagtcgct
     1021 gaccgggctg gctcgcacgc tgcgctgggt ccaccgcgat cagctcgagg tcgaccgcaa
     1081 gcgcctcgcg cgggaccgcg gcctggtcca ggcggcgatg cgcaagcgca agctcgcgtc
     1141 gcccgacgcg ctggccgcgg cgagcaagcc gcacgcgaag ctgatcgcga acctcaaggt
     1201 cgccgagcag caggtcgcgg cgctgacggc cgtgcaggac cacctcgtgt gtgacggctt
     1261 gctggcgacc aaggacctgg cgcgcgggca gttcgaccac gcgacggcgc tggcgctcaa
     1321 gcagtatcag ctgcgccact ggatcgtcgg atccggcgag ctcgacggcg agacgatgac
     1381 gagcctgcgc gaggacagcc gtgagctcga cttccggctc gcgctccggg tgctgcgcca
     1441 gcgggtcagc gacgccgcgg gcatcatcga ggacggctcg gcgcgcgccg agtgggcgcc
     1501 ggtgctcggg cgtcacctcg atggctcggc gctgcgctac cgcggcgccc acgagccgat
     1561 ggccaacggc gcgcccgacc tggtctcccc ggcgaccgag gcggcagcgc gcgcgctcgg
     1621 gtggcaggac tacgcgaccg cgcgggcctc gctgcgccag ctgctgtcgg gctcggagag
     1681 cctgcgcgtc gcggtgcagc tgccgccggt gccctcgtat cacagcaaga cgatggagct
     1741 gcgcgcggtg atcgacccgg gggaggtctc gtacgacgtc ccgaagcagc gcggggcctc
     1801 gcgcgcgggc ccgaaggcgg agcgccgccc ggtgctgatc gtgtacgcca aggacgggga
     1861 caaggaggtc gcgctggtgc gctggccgac gacgatcggc ggctggcagg acgagaagct
     1921 cgccaacggc gcgatcgtcc gccgctacaa gccctccgac accggccccc ggctgtggcg
     1981 cgacctcgtg gtcgccccgg tgtggtaccc gcccaagagc acgcccgaca acgagctggt
     2041 gcgcgcgcgc aacggcaagt gggagctcaa ggaggacctg atcggccccg gctaccgctc
     2101 ggcctacggc ctggcgatgc tgatccatca catggtggtg tcgaagcccg gcggcgacgt
     2161 gctctacgac cgcgcgatcc ggacccacgg ctcggcccac tatcagtcga tcacccgcgc
     2221 cgacagccac ggctgccatc gtacgtacaa tcaccagatc ctcggcctgg cgtcgttcct
     2281 gctgcatcac cacgcgtaca cggtcaaggg cccgttgcag gagaagtatg tccgcaaggt
     2341 ccggc
//
LOCUS       DASWNJ010001787         2261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_103334, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001787 DASWNJ010000000
VERSION     DASWNJ010001787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103334"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VGB85_29905"
     CDS             complement(<1..874)
                     /locus_tag="VGB85_29905"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0138351.1"
                     /translation="MNTFLTLLLNGISLGAIYALIALGFAIIFKASEVVSFMHGSLLL
                     LGAYTIAVLDTSIGFLPAVLVGIAATSGVGLLVERLLINRLRGAPVISLAILTIGIDI
                     MILTELTRRIGSDILNVAHPWGGDFIRIGGIGITENRLYAMLTAGVLIAIFFTAFKFS
                     SWGVAMRASAEDGETAALMGIKLGRVSALAWVIGAALAAVAGLFLVGSPTPGVTPGVW
                     AVALRAFPAAILGGLDSTGGALVGGMVIGIAEALAAGYQDQIAFLGRGFGDVVPYVVM
                     ILVLLVRPSGLFGTK"
     gene            complement(871..1824)
                     /locus_tag="VGB85_29910"
     CDS             complement(871..1824)
                     /locus_tag="VGB85_29910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0138352.1"
                     /translation="MDGVTAPAGTGARGQGAAERGVPPLVVEGVTVRFGALAALSEVS
                     FTVAPGTVHAIIGPNGAGKSTMFNVLSGVYRASEGTVRFGDHVLTGMRPHRIAKLRIA
                     RAFQNIALSGGQTVAENLMLGRHHLTTAGFLASGLRLPKATREGRQHGERVREIAEFL
                     ELGDKLHTPVGVLSYGDQKRVEVARALCTEPDLLLLDEPVAGMNAAETRRMAEAVLEI
                     RDALGISIVLVEHDMGMVMGIADRVTVLDFGRRIADGTPAEVQRDPEVIRAYLGSGDD
                     DEPAATGPAAAGGSGASPSGAGSTRPADGGSRRIATSEDPA"
     gene            complement(1812..>2261)
                     /locus_tag="VGB85_29915"
     CDS             complement(1812..>2261)
                     /locus_tag="VGB85_29915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0138353.1"
                     /translation="KVAAQKRVYDLFPVLADRRSGRAGLLSGGEQQMLAIGRALMAGP
                     KLLLLDEPSLGLAPRIISQIGSIVREINRQGTSVLLVEQNATMALGVADLAYVLDVGK
                     VSLSGAAAELAATDAVQRLYLGHAAGEEAEVQQAVARSAKTLSRWTA"
BASE COUNT          338 a          789 c          854 g          280 t
ORIGIN      
        1 ccttggtccc gaacaggccc gagggacgca cgagcaggac gaggatcatc acgacgtagg
       61 gcacgacgtc accgaagccg cggccgagga aggcgatctg gtcctggtag ccggcggcca
      121 gggcctcggc gatgccgatg accatgccgc cgaccagcgc cccgccggtc gagtcgaggc
      181 cgccgaggat cgccgcgggg aacgcccgca gcgccaccgc ccagaccccg ggcgtcacac
      241 caggggtcgg cgagccgacg aggaacagcc cggccacggc ggccagcgcg gccccgatca
      301 cccaggccag cgccgagacg cggcccagct tgatgcccat gagcgcggcg gtctcgccgt
      361 cctcggcgct ggcgcgcatc gcgacgcccc agctggagaa cttgaaggcg gtgaagaaga
      421 tcgcgatcag gacgccggcc gtgagcatcg cgtagaggcg gttctcggtg atgccgatgc
      481 cgccgatgcg gatgaagtcg ccgccccacg ggtgggcgac gttgaggatg tccgagccga
      541 tccggcgggt cagctcggtg aggatcatga tgtcgatgcc gatggtgagg atcgccaggc
      601 tgatgacggg cgcgccgcgc agccggttga tgagcagccg ctcgacgagc aggccgacgc
      661 cggaggtcgc cgcgatgccc acgagcacgg ccggcaggaa gccgatcgag gtgtcgagca
      721 cggcgatcgt gtaggcgccg agcagcagca gcgagccgtg catgaagctc acgacttcgc
      781 tggccttgaa gatgatggcg aagcccaggg cgatgagcgc gtagatcgcg ccgagcgaga
      841 tgccgttgag cagcagcgtc aggaaggtgt tcacgcgggg tcctcggagg tcgcgatgcg
      901 gcgggagccg ccgtcagcgg ggcgggtgga gccggcgccc gacggactgg cgcccgaccc
      961 accggctgct gcgggaccgg tcgctgcggg ctcgtcgtcg tcgccggagc cgaggtaggc
     1021 gcggatcacc tcggggtcgc gctgcacctc ggcgggcgtg ccgtcggcga tccgccggcc
     1081 gaagtcgagg acggtgacgc ggtcggcgat gcccatgacc atgcccatgt cgtgctcgac
     1141 gagcacgatg ctgatcccga gcgcgtcgcg gatctccagc accgcctcgg ccatccgccg
     1201 ggtctcggcg gcgttcatgc cggcgacggg ctcgtcgagc agcagcaggt caggctcggt
     1261 gcacagcgcc cgcgcgacct cgacccgctt ctggtcgccg tacgacagca cgccgaccgg
     1321 ggtgtgcagc ttgtcgccga gctcgaggaa ctccgcgatc tcgcggaccc gctcgccgtg
     1381 ctgccggccc tcgcgggtgg ccttgggcag gcgcagcccg gaggccagga agccggcggt
     1441 ggtgaggtgg tggcggccga gcatgaggtt ctcggcgacg gtctgcccgc cggacagcgc
     1501 gatgttctgg aacgcgcggg cgatccggag cttggcgatg cggtgcggcc gcatgcccgt
     1561 caggacgtgg tcgccgaagc gcaccgtccc ctcgctggcg cggtagacgc cggacagcac
     1621 gttgaacatc gtcgacttgc cggcgccgtt cgggccgatg atggcgtgga ccgtgccggg
     1681 ggcgacggtg aacgacacct cggacagcgc cgccaacgcg ccgaagcgca cggtgacgcc
     1741 ctcgacgacc agcggcggga caccgcgctc ggcggcgccc tgccctcggg cccccgtgcc
     1801 ggcgggcgcc gtcacgccgt ccaccgcgac agcgtcttgg cgctgcgggc cacggcctgc
     1861 tgcacctcgg cctcctcgcc ggccgcgtgg ccgaggtaga gccgctgcac cgcgtcggtg
     1921 gcggcgagct ccgcggccgc gcccgacagc gacaccttgc cgacgtccag gacgtacgcc
     1981 agatcggcga cgccgagcgc catcgtggcg ttctgctcca cgagcagcac cgaggtgccc
     2041 tgccggttga tctcccgcac gatcgagccg atctgggaga tgatgcgagg cgcgagaccg
     2101 agcgagggct cgtcgagcag cagcagcttc gggccggcca tgagcgcccg gccgatcgcg
     2161 agcatctgct gctcgccgcc cgacagcagc ccggcgcggc cgctgcggcg gtccgccagc
     2221 acggggaaca ggtcgtagac ccgcttctgc gcagcgacct t
//
LOCUS       DASWNJ010001788         4386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_243774, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001788 DASWNJ010000000
VERSION     DASWNJ010001788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243774"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VGB85_29920"
     CDS             complement(<1..207)
                     /locus_tag="VGB85_29920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138354.1"
                     /translation="MPSTGEPDSSSSLTDGLGGPGEGPTTGGPLATSSGSSGSTGGTG
                     GAVETTGDGGPSCGDDVVDAGEACD"
     gene            709..1875
                     /locus_tag="VGB85_29925"
     CDS             709..1875
                     /locus_tag="VGB85_29925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0138355.1"
                     /translation="MTPALPQIVRELFKYVPVIAWAMDKHGVCLLGEGSTLGGIGYHA
                     GQLVGLNLLEVFAQDPVSADFLRRALAGESFTVASEANGRTLSTRYEPMFDEHGVQNG
                     VVGITVDETLRLASERELATRAAALAEQAELLDLAHDAILVCRLDGTITYWNRGAERI
                     LGHTREQATGRNALELLHTALPVPLAEIQRALQTQGYWEGELTHARGDGEEVITASRW
                     VLRRGEGDATDSVLQIDTDITARKRAEQAEHRRQQEIIRAQAQAIEELSTPLIPITDE
                     ILVMPIIGMLDSIRAQQIMHTLLSGLAASRGKFAILDITGVPVVDTAVASAILRAAHA
                     ARLLGTEVILTGIRPEVAQTLVHIGAELGNIITRGTLQSGIKYALARGRSKLGV"
     gene            2058..3224
                     /locus_tag="VGB85_29930"
     CDS             2058..3224
                     /locus_tag="VGB85_29930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0138356.1"
                     /translation="MKPTLPQIVRELFNYLPVIAWAMDMDGVVLLAEGSTMGSIGFAP
                     GQLVGQNLLAVFGQNPASIEPMQRALAGEAFTQIGDSGGRTLSTRYEPMFDEHGVQNG
                     VIGITIDETDRLANDRELATRASALREQAELLDLAHDAIVVCRLDGTITYWNRGAERM
                     LGYTRDEAVGRHASELLRSVLPVPIADIQRAFATQGYWEGEVIHTSRDGEEVIVASRG
                     VLRRGEADTADSVLVIDTDITARKRAEQAEHRRQQEIIRAQAQAIEELSTPLIPITDE
                     ILVMPIIGMLDSIRAQQVMHTLLSGLAASRGKFAILDITGVPVVDTAVANAILQAAHA
                     ARLLGTEVILTGIRPEVAQTLVHIGAELGNIITRGTLQSGIKYALARGRSKLGA"
     gene            complement(3348..>4386)
                     /locus_tag="VGB85_29935"
     CDS             complement(3348..>4386)
                     /locus_tag="VGB85_29935"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0138357.1"
                     /translation="RYDEALTWRRHAHAVIARIGEGSPGHTRLLRAFGSVLLRQGRDG
                     DAAQVMTQALRLDSARPEQDERELIADLKGLGTAESIRGNYVESRQLLERALALVEQR
                     DGPRHPDIAPIANNLAGTLSVMGERAAAQALYERAIDITVAALGPDHPEVSTALTNLG
                     ASLGENGDLVGARELLERALAIDERVLGPEHPELAPGLHFLASVAIDANDLAAARKYA
                     ERALAIREKHFAPDNRLVAISRAEVGLVLYLQGRLPAAIRELERALAVLVAHADHGFP
                     EAVPQTRFTLAQALRDSGKHERARALATEAIAEYAAIGSEPARESIKDIEAWLATGPS
                     AVRGERRRGKK"
BASE COUNT          688 a         1742 c         1314 g          642 t
ORIGIN      
        1 gtcgcacgcc tcgccagcgt cgacaacatc gtcgccgcag gacggcccgc cgtcgcccgt
       61 cgtctccact gcgccgccgg tgccgccagt gctgccagag ctccccgaac tggtagcgag
      121 cggccccccg gtggtcgggc cctcgccggg cccacccagc ccatccgtca gcgagctcga
      181 gctgtctggc tcaccggtgg acggcacgcc gccgaggtcg acagtcccgc gtgacggcgc
      241 gtcgctgcaa ctggcgagcg acgcgaaaag tacggcgaac gagaacctgg cggagcgtcg
      301 aagcattcgt ctgtgtcctg atccgaccag gtgacacaac gaatacgaac tgtcacgaaa
      361 aatcgctcgc ggcaccaccc gggtctcaat atagtgatga ttcagcagct tttattttac
      421 accgccgaca gtttacgtaa ttcacttcac agtttgtaca gcgcagcgaa ctctcgttgc
      481 ccgcgtcatc gtcgtctctc gatctgcact cgacagcatc ggcgcccctt cgccgctgcc
      541 catcgcgccc acaccgccgc cgtcacgccg ctccccgcgc caatcggcga acgtggatga
      601 ccgcgccgac gctcgtcgcc acggacgggt gaccgcaaca aacgaccact gcgcgctgca
      661 ctcactgcgc gttgaaaacg tcgttatcgc aggtacattg cccgcgtcgt gacacccgcg
      721 ctgccgcaga tcgtccgtga gctcttcaag tatgtcccgg tcattgcctg ggcgatggac
      781 aaacatggcg tctgcctgct cggcgagggc tcgaccctcg gcggcatcgg ctatcacgcc
      841 gggcagctcg tcggcctcaa cctgctcgag gtgttcgccc aggaccccgt ctcggccgac
      901 tttctcaggc gcgccctcgc cggcgagtcc ttcaccgtgg cgagcgaggc caacggccgc
      961 acgctctcga cccgctacga gccgatgttc gacgagcacg gcgtacaaaa cggcgtcgtc
     1021 ggcatcaccg tcgacgagac cctccggctc gcgagcgagc gcgagctcgc cacccgcgcc
     1081 gccgccctcg ccgagcaggc cgagctcctc gacctcgccc acgacgccat cctcgtgtgc
     1141 cgcctcgatg gcacgatcac ctactggaac cgcggcgccg agcgcatcct cggccacacc
     1201 cgcgagcagg cgaccggccg caacgccctc gagctgctgc acaccgccct gccggtcccg
     1261 ctcgccgaga tccagcgggc cctgcagacc cagggttact gggaaggcga gctgacccac
     1321 gcccgcggcg acggcgagga ggtcatcacc gccagccgct gggtcctgcg tcgcggcgag
     1381 ggcgacgcca ccgactccgt cctgcagatc gacaccgaca tcaccgcccg caagcgcgcc
     1441 gagcaggccg agcatcgtcg ccagcaggag atcattcgcg cccaggccca ggcgatcgag
     1501 gagctctcca ccccgctgat cccgatcacc gacgagatcc tcgtcatgcc gatcatcggc
     1561 atgctcgact cgatccgcgc ccagcagatc atgcacaccc tgctgtcggg cctcgcggcg
     1621 tcccgcggga agtttgcgat cctcgacatc accggcgtcc ccgtcgtcga caccgccgtc
     1681 gccagcgcga tcctccgggc cgcccacgcc gcgcgcctgc tcggcaccga ggtcatcctc
     1741 accggcatcc gccccgaggt cgcccagacc ctcgtccaca tcggcgcaga gctcggcaac
     1801 atcatcaccc gcggcacgct ccagagcggc atcaagtacg ccctcgcccg cggccgcagc
     1861 aagctcggcg tctaaatcac cctatcgcgc cgccgcgttc tgcgacgtct tcgatcgcgt
     1921 cgacgcacgc atcaccgcga tcgcaaggcc atggagtgca gtacagcaca ccgatccgca
     1981 ccgccgaccg gatcatcgtt cgctgcactt cctgcgctgt ggccctctcc gggttacagg
     2041 tacattgccc tgctcccgtg aaacccacgc tcccccagat cgtccgcgaa ctcttcaatt
     2101 acctccctgt catcgcctgg gcgatggata tggatggcgt cgtgctgctc gccgagggct
     2161 cgacgatggg gagcatcggc ttcgcgcccg ggcagctcgt cgggcagaac ctgctcgccg
     2221 tgttcggcca gaaccccgcc tcgatcgagc cgatgcagcg cgccctcgcc ggtgaagcct
     2281 tcacgcagat cggcgacagc ggcggacgca ccctctcgac ccgctacgag ccgatgttcg
     2341 acgagcatgg cgtacaaaac ggcgtcatcg gcatcacgat cgacgagacc gaccgactcg
     2401 ccaacgaccg cgagctcgcc acccgcgcca gcgctctgcg cgagcaggcc gagctcctcg
     2461 acctcgccca cgacgccatc gtcgtgtgcc gcctcgacgg cacgatcacc tactggaacc
     2521 gcggcgccga gcgcatgctc ggctacaccc gcgacgaggc cgtcggccgc cacgccagcg
     2581 agctgctacg cagcgtcctg ccggtcccga tcgccgacat ccagcgggcc ttcgcgaccc
     2641 agggttactg ggaaggcgag gtcatccaca ccagtcgcga cggcgaggag gtgatcgtcg
     2701 ccagccgcgg cgtcctgcgt cgcggcgagg ccgacaccgc cgactccgtg ttggtgatcg
     2761 acaccgacat caccgctcgc aagcgcgccg agcaggccga gcaccgtcgc cagcaggaga
     2821 tcattcgcgc ccaggcccag gcgatcgagg agctctccac cccgctgatc ccgatcaccg
     2881 acgagatcct cgtcatgccg atcatcggca tgctcgactc gatccgcgcc cagcaggtca
     2941 tgcacaccct gctgtccggc ctcgcggcgt cccgcggcaa gtttgcgatc ctcgacatca
     3001 ccggcgtgcc cgtcgtcgac accgccgtcg ccaacgcgat cctccaggcc gctcacgccg
     3061 cgcgcctgct cggcaccgag gtcatcctca ccggcatccg ccccgaggtc gcccagaccc
     3121 tcgtccatat cggcgcagag ctcggcaaca tcatcacccg cggcacgctc cagagcggca
     3181 tcaaatacgc gctcgcccgc ggccgcagca agctcggcgc ctgaaatata tagatacggg
     3241 accgggcggc ctggacggcc gcgacgcata gtgatagagc gccgctcgcc gcggccccgt
     3301 tccaggtagt cgccgctccc caccgaaccg tcacgcgccg cgacgattta ttttttgcct
     3361 cgccgccgct caccgcgcac cgcgctgggc ccggtcgcca gccaggcctc gatatccttg
     3421 atgctctccc gcgccggctc gctgccgatc gccgcgtatt ccgcgatcgc ctcggtcgcc
     3481 agcgcccgcg ctcgctcgtg cttgccgctg tcgcgcagcg cctgggccag ggtaaagcgc
     3541 gtctgcggca ccgcctccgg gaacccgtgg tcggcgtgcg cgaccagcac cgccagcgcc
     3601 cgctcgagct cgcggatcgc cgccggcagc cgcccttgca ggtacaacac cagcccgacc
     3661 tccgcccgcg agatcgctac caggcggttg tccggcgcga agtgcttctc ccggatcgcc
     3721 agcgcccgct ccgcgtactt gcgggccgcc gccaggtcgt tcgcgtcgat cgccaccgac
     3781 gcgaggaagt gcaggcccgg cgccagctcc gggtgctccg ggccgagcac ccgctcgtcg
     3841 atcgccagcg cccgctcgag cagctcgcgc gcgccgacga ggtcaccgtt ctcgccgagc
     3901 gacgcgccga ggttcgtcag cgccgtcgac acctccggat gatcgggccc cagcgccgcc
     3961 accgtgatgt cgatcgcgcg ctcgtacagc gcctgcgcgg ccgcgcgctc gcccatcacc
     4021 gacagcgtgc ccgccaggtt gttggcgatc ggcgcgatgt ccgggtggcg cggcccgtcg
     4081 cgctgctcga ccagcgccag cgcccgctcg agcaattgcc tcgactcgac atagttgccg
     4141 cggatcgact ccgccgtccc gagccccttg aggtccgcga tcagctcgcg ctcgtcctgc
     4201 tcgggtcgcg cgctgtcgag acgcagcgcc tgcgtcatca cctgcgccgc gtcaccgtcg
     4261 cgaccctgcc gcaacagcac cgaaccaaag gcccgcagca gccgcgtgtg cccggggctg
     4321 ccctcaccga tgcgggcgat caccgcgtgc gcgtggcgac gccacgtcag cgcctcgtcg
     4381 tagcgc
//
LOCUS       DASWNJ010001789         3289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_597390, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001789 DASWNJ010000000
VERSION     DASWNJ010001789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597390"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..1045
                     /locus_tag="VGB85_29940"
     CDS             287..1045
                     /locus_tag="VGB85_29940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138358.1"
                     /translation="MRRLLLLLTPLACNAPAPGDEADTSSGETTGAPTGESSGTGEVD
                     THDTDASPVMFTCEPGVTPSDPYIDCVESFTPEGAVFGQDRFPQVVFGPPHAGAGGTG
                     SVDVLSLGCGGEITLFFDAPGIVDGPGADFIVFENPLPAGDATFVEPARVLVSADGLT
                     WHAFPCDPAGEHPPVGCAGVARVFAGPDNMLDPTDPATAGGDAFDLAQLGLAGARYVR
                     LLDVGEVYYKGRTWCGGGGGGFDLDAIAVVHGGV"
     gene            1051..1632
                     /locus_tag="VGB85_29945"
     CDS             1051..1632
                     /locus_tag="VGB85_29945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138359.1"
                     /translation="MKIAVRHRQAADSIRRKQPGVAVVDLTSRGPQPWVKFSPFYPHG
                     GIPVPGTPNRFAASVEGLWQALKVFERADVDERTLDITTMKGIKRTTAKFGRVLGHRE
                     GLHGTRLLGYLEARREIYLPAYGLVLARDLTSELDQLRTLASGPAGVVLLDYETNTDI
                     DDLTSPLSHAGLVMRWLQGDWSASARAADTSAS"
     gene            complement(1581..1739)
                     /locus_tag="VGB85_29950"
     CDS             complement(1581..1739)
                     /locus_tag="VGB85_29950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138360.1"
                     /translation="MRDNDVRDELRLRFEERAAICEYDGKLPRAEAERVAYDALVSAA
                     RALADQSP"
     gene            complement(1990..3018)
                     /locus_tag="VGB85_29955"
     CDS             complement(1990..3018)
                     /locus_tag="VGB85_29955"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HEY0138361.1"
                     /translation="MAGPRRNVAPTNTLLALLALAPLAACSFDSAGLGQSSEGPGTDS
                     PATSGQTTGGADTTTGPTTGPTTGPTTQTTAGSGASGTESSEAGDSSGAVDPGTSSSA
                     TDGTSTTDVTASTGGDTTTGGGSSSSGSCVEQAFFADGDGDGFGDPDKLVMACEQPDA
                     HVGNSDDCDDSDDAVNLGADEICDGKDNDCDTYEDEFAAGTNLDCEGCKMALYEGAVY
                     HFCGTDEKWASAKSSCEARGAALVDDNDMEEHDWLVAQLPVDSGPWHIGGQAPGGDDK
                     FVWLDGTAIPNPDPRWGALRPAPGGGTNYLALVSAGNLIFWDAYDGRWYDLHGNSENP
                     YICQGPLH"
BASE COUNT          431 a         1216 c         1157 g          485 t
ORIGIN      
        1 gaacgcctcg ccgccgcgga gatctcgggg gacctggcac tggtcacgtc gcgcacgccc
       61 gacagcgtag gcccgcgtgc tccgtccgag aagactgaga cgttggcgca caattcgcca
      121 gccggcagcc tcgacctccg cccccgggcc ttcatacgac cccgtggcgg tttttacagg
      181 tcgctgcgca accgccgcta gcccacgatc gcggccctga tgccacctgc gcgcacaagt
      241 cagtcgacgc gttgtcccag gcgcaccagc cgctaccatc cgtccgatgc gccgcctcct
      301 gctcttgctg acacccctcg cctgcaacgc gcccgcgccc ggcgacgagg ccgacacgag
      361 cagcggcgag accacgggcg cgccgacggg cgagagcagc gggaccggcg aggtcgacac
      421 gcacgacacc gacgcgagcc cggtcatgtt cacctgcgag cccggcgtca cgccgagcga
      481 cccctacatc gactgcgtgg agtcgttcac gcccgagggt gcggtgttcg ggcaggaccg
      541 gttcccgcag gtcgtgttcg gtccgccgca cgcgggcgcc ggcgggaccg gcagcgtcga
      601 cgtgctctcg ctcggctgcg gcggcgagat cacgctgttc ttcgacgcgc cggggatcgt
      661 cgatggaccg ggggccgact tcatcgtctt cgagaacccg ctgcccgccg gcgacgcgac
      721 cttcgtcgag ccggcgcgcg tgctcgtcag cgccgacggg ctcacctggc acgcgttccc
      781 ctgcgacccc gccggcgagc acccgccggt cggctgcgcc ggggtcgcgc gcgtgttcgc
      841 cggtccggac aacatgctcg acccgaccga ccccgcgacc gccggcggcg acgccttcga
      901 cctcgcgcag ctcggcctcg ccggggcgcg ttatgtccga ctgctcgacg tcggcgaggt
      961 ttattataag ggccggacgt ggtgcggagg cggcggtggc ggcttcgacc tcgacgcgat
     1021 cgccgtcgtc cacggaggtg tatgagcacg ttgaagatcg ccgtccgtca tcgccaggcc
     1081 gccgactcga tccgccgcaa gcagcccggc gtcgcggtcg tcgacctgac ctcgcgtggg
     1141 ccgcagccgt gggtcaagtt cagcccgttc tacccgcacg gcggcatccc cgtgcccggc
     1201 acgccgaacc gcttcgcggc gagcgtcgag gggctgtggc aggcgctcaa ggtgttcgag
     1261 cgcgccgacg tcgacgagcg cacgctcgac atcacgacga tgaagggcat caagcggacc
     1321 accgcgaagt tcggccgcgt gctcggacat cgcgagggcc tgcatggcac gcgcctgctc
     1381 ggctacctcg aggcgcgccg cgagatctac ctaccggcgt acggcctcgt gctcgcgcgc
     1441 gacctgacca gcgagctcga ccagctccgc accctggcga gcggcccggc cggcgtcgtc
     1501 ctgctcgact acgagaccaa caccgacatc gacgacctca cgagcccgct gtctcacgcg
     1561 ggcctggtga tgcgctggct tcagggcgac tggtccgcga gcgcgcgggc ggccgacacg
     1621 agcgcgtcgt aggcgacgcg ctcggcctcg gcacgcggca gcttgccgtc gtactcgcag
     1681 atcgcggcgc gctcctcgaa gcgcaggcgc agctcgtcgc gcacatcgtt gtcacgcacg
     1741 ccggcgatca acgccgaggc ctgacgcgcc catcgagaca gtgaggtcgt gctcgccaac
     1801 gcgaggcgtc atgccgggcg cgcgagggcc ccgcaacgcg cggccccgcg cgcggcaatt
     1861 gtccctcagg atgcctctga tggcctgaat tcgaccgccg cgcgatcgca cgcccgggcc
     1921 cctgcgcgct tcgggggcgc gcagggcccg gccgagtacg cgcgctcgcg ctcatgcgcg
     1981 cgccttcgct cagtgcagcg ggccctggca gatgtagggg ttctcgctgt tgccgtgcag
     2041 gtcgtaccac cggccgtcgt aggcgtccca gaagatcaag tttccagcgg acacgagggc
     2101 caggtagttg gtgccgccgc cgggcgcggg ccggagcgcg ccccaccgcg gatcggggtt
     2161 ggggatcgcg gtgccgtcga gccacacgaa cttgtcgtcg cccccgggcg cctgaccgcc
     2221 gatgtgccag gggccgctgt cgaccggcag ctgggcgacc agccagtcgt gctcctccat
     2281 gtcgttgtcg tcgacgagcg ccgcgccgcg ggcctcacag gagctcttgg cgctggccca
     2341 cttctcgtcc gtcccgcaga agtggtagac cgcgccctcg tagagcgcca tcttgcagcc
     2401 ctcgcagtcg aggttggtgc ccgcggcgaa ctcgtcctcg taggtgtcgc agtcgttgtc
     2461 cttgccgtcg cagatctcgt cggcgccgag gttgaccgcg tcgtcgctgt cgtcgcaatc
     2521 gtcgctgttg ccgacgtgcg cgtcgggctg ctcgcaggcc atcaccagct tgtcggggtc
     2581 gccgaagccg tcgccgtcgc cgtcggcgaa gaaggcctgc tcgacgcaac tacccgagct
     2641 cgagctgccg ccgccggtcg tcgtgtcgcc gccggtgctc gcggtcacat cggtggtcga
     2701 ggtcccatcg gtggccgagg agctggtgcc agggtcgacc gccccgctgc tgtcgccggc
     2761 ctcgctcgac tcggtgccgg acgctccgga gccggcggtg gtctgcgtgg tcggaccggt
     2821 cgtcggcccg gtggtcggtc cggtcgtcgt gtcggcgccg ccggtggtct gcccggaggt
     2881 cgcggggcta tccgtgccgg gcccctcgct gctctggccg aggccggcgg agtcgaagct
     2941 gcaggcggcc agcggggcca gtgccagcag cgcgaggagg gtatttgtag gggcaacgtt
     3001 gcgtcgagga ccggccatgg tccctggctt atgccccgcg atcacggggg tagcaagccg
     3061 accatcgaga agcggccgtg gagattgcgc cagggcacat gcaaagcgac aactcgggta
     3121 cactggcgtc atgcgcatga cccctggcaa cgcgagccct ctggtcctgg ctgcgatcct
     3181 tctcagctgc gcgccgacca cgagcgaggc gcagccccag cccgcgccgt cgctcgcggc
     3241 gacgtctgcc gcgccgccgg caccgcggcc gacgccggcc cccccggcg
//
LOCUS       DASWNJ010001790         2357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_103344, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001790 DASWNJ010000000
VERSION     DASWNJ010001790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103344"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VGB85_29960"
     CDS             complement(<1..224)
                     /locus_tag="VGB85_29960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEY0138362.1"
                     /translation="MKPATHLQLLPTEAPVGARAAGTGLDDDLALRACAGDVSAWSEI
                     YARHYPQLLRQLRHLTGDLAVAEELAQETFA"
     gene            complement(313..1419)
                     /locus_tag="VGB85_29965"
     CDS             complement(313..1419)
                     /locus_tag="VGB85_29965"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY0138363.1"
                     /translation="MGTPPANSNSSPDPVPRMTALEALERAQWIAFAPFVFQATVALR
                     DHGLLLAIEGSPRGLTLDEAAAAVGLPVYGTRVLLEAGLGIGVLVEVEGRYRLTKVGT
                     FVQHDAMTRANMDFTRDVNYAGIAQLGESVKTGKPEGLKVFGEWATVYEGLAYLSEPV
                     RRSWFAFDHHYSDEAFGAALPLVFRDRPRKILDIGGNTGKWATACLRRDPEVEVTLLD
                     LPGQLRNAEQSLGAAGLLDRAHFHPANLLDEATEIPRGFDAIWMSQFLDCFSEAQIVS
                     ILRRCVAALGPDAKIYILEAFWDCQRFPAASFCLQMTSLYFTAIANGNSQMYRSTTFL
                     ACVHAAGLEVERQHDELGLSHTLLVCRKAGSAGV"
     gene            complement(1694..>2357)
                     /locus_tag="VGB85_29970"
     CDS             complement(1694..>2357)
                     /locus_tag="VGB85_29970"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEY0138364.1"
                     /translation="VLAPYRLLTFVGGFSFGDHVASGRILANRLRDRMGDRLLRFVAD
                     GGLCLGICNGFQTLVKLGLLPGDGPGLRQSVTLVNNDRLGYWDAWVRLAIDPQSPCVF
                     TRGLTDIELPSRHGEGKLVYADAAQRSVLEDRHQIPLRYVDAAGRPTETWPDNPDGSP
                     NGAAGLCDPSGRIFGLMPHPEAYLYPENHPRWIAQRAAGTLPKAGLGLQLLANGVRAA
                     IG"
BASE COUNT          372 a          796 c          857 g          332 t
ORIGIN      
        1 gcaaaggtct cctgcgcgag ctcctccgcg accgcgaggt cgccggtcag atgccggagc
       61 tggcgcagca gctgcggata gtgccgcgcg tagatctcgg accaggccga gacgtcaccc
      121 gcgcaggcgc gcagcgccag gtcgtcgtcc aggcccgttc cggccgcgcg cgcccccacg
      181 ggcgcctccg tcggcaggag ctgcaggtgc gtggccggct tcatcgcggg agaggtccag
      241 cgtggcctta ccgcgaggct ccggtttctt acagactatt ttccgccgac gcgcggcccc
      301 gcaaaaacgc cgtcagacgc ccgcggagcc ggccttgcgg cagaccagca gcgtgtggct
      361 caggccgagc tcgtcgtgct ggcgctcgac ctcgaggccg gccgcgtgca cgcacgcgag
      421 gaaggtcgtc gaccggtaca tctggctgtt gccgttggcg atcgccgtga aataaagcga
      481 ggtcatctgc aggcagaagc tcgcggccgg gaaccgctgg cagtcccaga acgcctcgag
      541 gatgtagatc ttcgcgtcgg gcccgagcgc ggcgacgcag cggcgcagga tcgagacgat
      601 ctgcgcctcc gagaagcagt cgaggaactg actcatccag atcgcgtcga agccgcgcgg
      661 gatctcggtc gcctcgtcga gcagattggc cggatgaaag tgggcccggt cgagcaggcc
      721 ggccgcgccg aggctctgct ccgcgttgcg cagctggccc ggcaggtcga gcagggtgac
      781 ctcgacctcg gggtcgcgcc gcagacacgc ggtcgcccac ttgcccgtgt tgccgccgat
      841 gtcgaggatc ttgcgcggac gatcgcgaaa caccagcggt aaggcggccc cgaaggcctc
      901 gtccgagtag tggtgatcga aggcgaacca cgagcgccgc acgggctccg acaggtacgc
      961 gagaccctcg tagacggtcg cccactctcc aaagaccttg agcccctcgg gcttgccggt
     1021 cttgaccgac tccccgagct gcgcgatgcc ggcgtagttg acgtcgcgcg tgaagtccat
     1081 gttggcgcgc gtcatcgcgt cgtgctgcac aaaggtgccg accttggtca gccggtagcg
     1141 accctcgacc tcgacgagca cgccgatgcc gaggccagcc tcgagcaaca cgcgcgtgcc
     1201 atacaccggg aggccgacgg ccgcggcggc ctcgtcgagc gtcaggcccc gcggactgcc
     1261 ctcgatcgcc agcaacagcc cgtggtcacg cagagccacg gtcgcctgaa agacgaacgg
     1321 ggcgaacgcg atccactgcg cgcgctcgag ggcctcgagc gccgtcatgc gcggcacggg
     1381 gtcgggagag ctgttcgagt tggcgggagg cgtgcccaag acccgtgcag gatatacgcc
     1441 tcgcggctgg tgtacagcgg tcaatggcct gggagggcgt gtgcggccgc ctcatatcgt
     1501 cggcgccccg ctcgtctccg catcgaggct cacccgcgtg tgtacgacgg gcggcacaac
     1561 agtcatcgat gcaggcgctt ctccagacgt cagccctcca cgagaacgag cagagaccgc
     1621 cacgggagcc ggtttagccg atcgccgcgc gtacgcattg gcagcacttg aatgccgacc
     1681 gcgcgggccg ggttcacccg atcgccgcgc gcacgccgtt ggcgagcagc tgcaggccga
     1741 ggccggcttt gggcagcgtg ccggcggcgc gctgggcgat ccatcgcggg tggttctcgg
     1801 ggtagaggta ggcctccggg tgcggcatca ggccaaagat gcggccgctt ggatcgcaca
     1861 ggccggcggc gccatttggc gagccgtcgg ggttgtcggg ccaggtctcg gtcgggcggc
     1921 cggcggcgtc gacgtagcgc agcgggatct ggtgcctgtc ctcgaggaca gaccgctggg
     1981 cggcgtcggc gtagaccagc ttgccctcgc cgtgacggct cggcagctcg atgtccgtca
     2041 gcccgcgggt gaacacgcag gggctctgcg ggtcgatcgc caggcgcacc caggcgtccc
     2101 agtagccgag gcggtcgttg ttgacgagcg tgacgctctg gcgcagcccc gggccgtcgc
     2161 cgggcagcag gccgagcttg accagcgtct gaaagccgtt gcagatgccg aggcacaggc
     2221 cgccgtcggc gacgaagcgc agcaggcggt cgcccatgcg gtcgcgcagg cgattggcga
     2281 ggatccggcc gctggcgacg tggtcgccga acgagaagcc gccgacaaag gtcagcaagc
     2341 gatacggcgc gagcacg
//
LOCUS       DASWNJ010001791         1858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_280029, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001791 DASWNJ010000000
VERSION     DASWNJ010001791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280029"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1622)
                     /locus_tag="VGB85_29975"
     CDS             complement(<1..1622)
                     /locus_tag="VGB85_29975"
                     /inference="COORDINATES: protein motif:HMM:NF012973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HEY0138365.1"
                     /translation="MTADSRSTTADALRLALAGWPRLVPAAWVCAMLACMLVPLPTAA
                     VDLLLSLSLAAAVLLLVASLAVRRSAEFLTFPALLLLVTLSRLALNVATTRLILSQAD
                     AGRVIDAFAGLVVRGDIVVGAVMFAVITAVQFLVIARGSERVAEVAARFALDGLPGQQ
                     AAIDADLRAGAISAEEASRRRSALLERSSFYGAMDGAARFVRGDAIAGLAITAINLVG
                     GVAVGSLRHGMSVADSLELYGRLTVGDGLLAQIPALLVSLAAGVLVTRVDRDAGARLQ
                     VLAWLEPAMLAIPAALLVGLAVVPGMPRLAFLATALGLVCGGLWLAVRRDSGPVEEPA
                     AQIVVRLARASPEALRSLRPPLSALRDRCAGALGLELPAIVVVAAPHGEGSEVRLGAR
                     LLAELAPADLVESAGPSEHVPEDRQGELSADAVVVACFRAIMGQAEAMLDLQQLERAI
                     ERTRARHPAVVREALRVLAPPELLGLCRGLLRERLPLPPLPALLEVVATEPRLRVASE
                     RPRWGEHLREQLAGLWLRDLVRAHARLGPVRWV"
     gene            complement(1679..>1858)
                     /locus_tag="VGB85_29980"
     CDS             complement(1679..>1858)
                     /locus_tag="VGB85_29980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138366.1"
                     /translation="RGYRWRPPITHLTLRLMGEEVTVDDTKARAEIGYTGAVKPQDGL
                     TEMRHARATTVSETP"
BASE COUNT          265 a          766 c          646 g          181 t
ORIGIN      
        1 acccagcgca ccggccccag gcgagcgtgc gcgcggacca ggtcgcgcaa ccacagcccc
       61 gcgagctgct cgcgcaggtg ctcgccccag cgcggccgct cgctggcgac ccgcagccgc
      121 ggctcggtcg ccaccacctc gagcagcgcc ggcagcggcg gcagcggcag gcgctcgcgc
      181 aacaggcccc gacacaggcc gagcagctcc ggcggcgcga gcacccgcag cgcctcgcgc
      241 acgaccgccg ggtgcctcgc ccgggtccgc tcgatcgccc gctcgagctg ttgcaggtcc
      301 agcatcgcct cggcctggcc catgatcgcc cggaagcagg cgacgaccac cgcgtccgcc
      361 gagagctcgc cttgcctgtc ctccgggaca tgctccgagg gacctgcgct ttcgaccagg
      421 tccgcgggcg ccagctcggc gagcaggcgc gccccgaggc ggacctcgct gccctccccg
      481 tgcggcgccg cgacgacgac gatcgccggc agctcgagcc cgagcgcccc ggcgcagcga
      541 tcgcgcagcg ccgacagcgg cggacgcagc gatcgcagcg cctccgggct cgcccgcgcg
      601 agccgcacca cgatctgcgc cgccggctcc tcgaccggcc cgctgtcgcg gcgcaccgcc
      661 agccacagtc cgccgcacac cagcccgagc gcggtcgcca ggaacgcgag gcgcggcatg
      721 cccggcacga ccgcgaggcc gaccaacagc gccgccggga tcgccagcat cgccggctcg
      781 agccacgcga gcacctgcag gcgcgcgccg gcgtcgcggt cgacgcgggt gaccagcacg
      841 ccggccgcga ggctgaccag cagcgccggg atctgggcca gtaacccatc gccgaccgtc
      901 agccggccgt acagctcgag gctgtcggcg accgacatgc cgtggcgcag gctgccgacc
      961 gcgaccccgc cgacgaggtt gatcgccgtg atcgccaggc ccgcgatcgc gtcgccgcgc
     1021 acgaagcggg ccgcgccgtc catcgccccg tagaagctcg agcgctcgag cagcgcgctg
     1081 cgccgccgcg aggcctcctc cgccgagatc gcccccgccc gcaggtcggc gtcgatcgcc
     1141 gcctgctggc ccggcagacc gtcgagcgcg aagcgggccg cgacctcggc gacgcgctcc
     1201 gagccgcgcg cgatcaccag gaactgcacc gcggtgatca ccgcgaacat caccgcgccg
     1261 acgacgatgt cgccgcggac caccagcccc gcgaacgcgt cgatcacccg ccccgcgtcc
     1321 gcctggctca ggatcaggcg cgtcgtcgcg acgttgaggg cgagccgcga cagcgtcacc
     1381 agcagcagca gcgccgggaa cgtcaggaac tcggcgctgc ggcgcaccgc caggctcgcg
     1441 accagcaaca acaccgccgc cgccaggctc aatgagagca gcaggtcgac ggccgccgtc
     1501 ggcagcggca ccagcatgca cgccagcatc gcgcacaccc acgcggccgg gaccagccgc
     1561 ggccagccgg cgagggcgag tcgcagcgcg tcggccgtgg tgctgcggct gtcggcggtc
     1621 acccgcgcac gctagcggcc ccgaccgccc cggggcaagt cgtcggcccg aaggatcttc
     1681 agggcgtctc gctgacggtg gtcgcccgcg cgtgccgcat ctcggtgagg ccgtcttgcg
     1741 gcttcaccgc gccggtatac ccgatctcgg cgcgggcctt cgtatcgtcg accgtgacct
     1801 cctcgcccat caggcgcagc gtcaggtggg tgatcggcgg gcgccagcga tagccccg
//
LOCUS       DASWNJ010001792         3468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_210353, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001792 DASWNJ010000000
VERSION     DASWNJ010001792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210353"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VGB85_29985"
     CDS             complement(<1..416)
                     /locus_tag="VGB85_29985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138367.1"
                     /translation="MLHVYFAGILALSASPSTDDAADTLSARAASIEAEVVALTQRNE
                     AMQAGKLAAQAMRGSDLSNHDRYHLGALAFDAYMVAHINNPSVAALCEARRVARAMAK
                     LATTPNEKTSARGLVGQVTSELAKLDPPGRCEPQAAR"
     gene            549..3233
                     /locus_tag="VGB85_29990"
     CDS             549..3233
                     /locus_tag="VGB85_29990"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0138368.1"
                     /translation="MVDAADPRGVGPRQLPWTEYDVPGTPAEEGPPYGIGGRYVIQQL
                     IGRGGMASVYLGRDRRLKRTIAIKILRRGIEVERFEREAVILANLRHDHIADVYDSGV
                     LDDGRPYFVTEYLDGVNLGRLLAVAPLPWEVVLEIGLQVASALRAVHQRGVIHRDVKP
                     ENIVLLRPLKGELRVKLVDFGVARLTTRWDEVATRDTPPKERRRTQEGMALGTEGYIS
                     LAASMGQVMPETDVYALGVTLFKLCTGQHPYETELDRIHQRDIAPELAHVILRAIDVD
                     PEREMGTAEALHVALTKAASGHPPETLDQECRAYLGRVFFRDEPAAPQGPKAAPSAPT
                     VVPARPAALPPRPDDALAAGRGPLAPSSREELRQWVGRVLDDRFELEGLLGDGGSAVV
                     FDAWDMRFRQRAAIKVLLPEQANDAAALQRFDHEARVLMKVRQEHLVAAFDRGTTTEG
                     LHYLALEHLDGAHLGKIRGASWLHWPVVLEIGAQAAKALDALHRVGVLHRDVVPTNLV
                     WVRDPGRPVHIKLIDASHCKLLPAWYERERRDATRPRDRLLTREGLIFGDRRFRPPEA
                     GVRPPHPREDIFGLGVSLYLLLTGKYPYPAGMPAFGQTIEPVPLAEANPGADVPAELA
                     QLVHDAIQLDPELRPESAEEFFDQWMGVAESLDPNAFHDYVLTGAIKLGECPEAQRIP
                     DGASFPEWYKPRRGARPERREPAPELGEVRPEPAPLAQASSLASPAPDVLRAPADPAR
                     LQPRSDRRVAVSWRAVVTYVAVAAAATLIAVATWPASPDPVSTTGVVPLVAPDPADAP
                     ASPSLADVQVAISSPAVAQQSDTDRLRDALRAGCPGLHGSLRLSVRIDEHRPQVLRVD
                     SLPLDLDRRPEHRCISDHLAAVQVGPRSTATQLVVVKL"
BASE COUNT          516 a         1223 c         1174 g          555 t
ORIGIN      
        1 cgagctgcct gcggttcgca ccgaccgggc gggtcgagct ttgccagctc gctggtcacc
       61 tgccccacga gcccgcgcgc ggacgtcttc tcattcgggg tagttgccag cttcgccatg
      121 gcccgcgcga cgcgacgggc ctcgcagagc gccgcgacgc tggggttgtt gatatgcgcc
      181 accatgtacg cgtcgaacgc cagggccccc aggtggtaac gatcgtggtt ggagaggtcc
      241 gagccacgca tcgcctgcgc ggcgagcttg ccggcctgca tcgcctcgtt ccgctgggtc
      301 agcgcgacga cctccgcctc gatgctcgcg gccctcgccg acagagtgtc ggccgcgtcg
      361 tccgtcgatg gcgacgcaga aagcgccagg atgcccgcga aatacacgtg cagcatggcc
      421 tgctttctac cacggagctc gtcgcaaggt cacgagctcg aagctctgct cgcatttccc
      481 aaaggacagg tcagcgatct cggcgcgtct gcttgactcg ggtgtgctcc gggaagatcc
      541 tcacctgcat ggtcgacgcc gccgatccga gaggagtggg ccccaggcaa ctgccgtgga
      601 ccgaatacga cgtccccgga acgcccgcgg aggagggccc gccgtacggc atcggcggtc
      661 gttatgtgat ccagcaactg atcggtcgcg gagggatggc gagcgtctac ctcggtcggg
      721 atcgacggct caagcgtacg atcgcgatca agatccttcg gcgcggcatc gaggtcgagc
      781 gtttcgagcg cgaggccgtc atcctcgcca acctgcgcca cgatcatatc gctgacgtct
      841 atgacagcgg cgtgctcgac gacggccggc cttactttgt caccgagtac ctcgatggcg
      901 tcaacctcgg ccggctcctc gcggtcgcgc cgctgccgtg ggaggtcgtc ctcgagatcg
      961 gcctgcaggt cgcgtcggcc ctgcgagcgg tgcatcagcg cggtgtcatt caccgcgacg
     1021 tcaagccgga gaacatcgtg ctgctgcggc cgctcaaggg cgagctgcgc gtcaagctcg
     1081 tggacttcgg ggtcgcgcgg ctgacgaccc gctgggacga ggtcgccacg cgcgacacgc
     1141 cgccgaagga acgacggcgc acgcaggagg gcatggcgct ggggaccgag ggctacatct
     1201 cgctcgccgc gagcatgggc caggtgatgc cggagaccga cgtgtacgcg ctcggggtca
     1261 ccctcttcaa gctctgcacg ggccagcacc cctatgagac ggagctcgat cgcattcacc
     1321 aaagagacat cgcccccgag ctcgcgcacg tgatcttgag agcgatcgac gtcgatcccg
     1381 agcgggagat ggggaccgcc gaggcgctgc acgtcgcgct caccaaggcc gcttccgggc
     1441 acccaccgga gaccctcgat caggagtgca gggcatatct cggccgcgtg ttttttcgcg
     1501 acgaaccagc cgcaccgcaa gggccgaaag ccgcgcccag tgcccccacg gtggtgcccg
     1561 cacgccccgc cgctttgccg cctcgccccg acgacgcgct cgcagcgggg cggggaccgc
     1621 tggctccgag cagccgggag gagctgcgcc agtgggtcgg ccgcgtgctg gatgaccggt
     1681 tcgagctcga aggattactc ggtgacggcg ggtcggcggt ggtcttcgac gcctgggaca
     1741 tgcgctttcg ccagcgggcc gcgatcaagg tcctgttgcc ggagcaggcc aatgatgcgg
     1801 ccgcgctcca gcgcttcgac cacgaggccc gcgtgttgat gaaggtgcgc caggagcacc
     1861 tggtcgccgc gttcgaccgt ggcaccacga ccgagggcct gcattacctc gcgctcgagc
     1921 acctcgacgg cgcgcacctc ggcaagatcc gcggcgcgtc ctggctgcac tggccggtcg
     1981 tcctcgagat cggagcgcag gctgcgaagg cgctcgatgc cctgcaccgg gtcggcgtgc
     2041 tccaccgaga tgttgtcccg accaacctcg tctgggtccg cgatccgggg cgccctgttc
     2101 acatcaagct gatcgacgcg agccattgta aactgctgcc ggcctggtac gagcgcgagc
     2161 gtcgcgacgc cacgcgcccg cgcgaccgac tgctgacgcg cgagggcctg atcttcggcg
     2221 accgtcgatt ccgaccgccg gaggccggcg tgcgtccgcc gcatcctcgc gaggatatct
     2281 tcggtctcgg cgtatcactg tacttgctcc tcacgggcaa gtatccgtac ccggccggaa
     2341 tgcctgcatt tggtcagacg atagaaccgg tgccgctcgc cgaggccaac ccgggcgccg
     2401 atgtgccggc cgagctcgcc cagctggtcc acgacgcgat ccagctcgac ccggagttgc
     2461 gacccgagag cgccgaggaa ttcttcgacc agtggatggg cgtagccgag tcgctcgacc
     2521 cgaacgcctt tcacgattat gtgctcaccg gcgcgatcaa gctcggcgaa tgccccgagg
     2581 cgcagcggat cccggacggg gccagcttcc cggagtggta caagccgcgc cgaggagcgc
     2641 ggcccgaacg tcgggaacct gcgccggagc tcggcgaggt gagacccgag cccgccccgc
     2701 tggcgcaggc gtcgtccctc gcatcgcccg caccggatgt gctgcgtgct ccggcggacc
     2761 cggctcggct gcaaccccgg tctgaccgcc gcgtcgctgt gtcatggcgg gctgtcgtca
     2821 cgtacgtcgc cgtcgcggcc gccgcgacgc tcatcgctgt cgccacttgg cccgcgagtc
     2881 ccgatcctgt ttcgaccaca ggcgtggtgc cactcgtggc tccggacccg gcagacgcgc
     2941 ctgcatcccc ctcgcttgcg gatgttcagg tcgcgatctc ctcccccgcg gtcgcgcagc
     3001 agtccgatac agaccgctta cgtgacgctc tgcgcgccgg gtgcccgggc ctgcacggct
     3061 cgttgcgcct cagcgttcgg atcgacgagc accgccccca ggttctgcgc gtggattcgc
     3121 tgccgctgga tctcgaccgg cggcccgagc atcgctgcat tagcgaccat ctggcggcgg
     3181 tgcaagtcgg acctcgttcg acggcgaccc agctcgttgt cgtcaagctg tagatctcgg
     3241 gcaccgcgcc gggcggtgcg cggctggcgg gaacgacgcg gccatcccgg ccgcgatctg
     3301 cggtacaagg cgccggcacg atgccagccc acacacccag agtcgggatc gacttcggca
     3361 catcgagctc cgcgctggcg ttcgcggatg ccggaggtca ggtccacttc gcgcagctgc
     3421 cgaccctcgg ggtgctcggt ccgagctggc gcacgctgct gttcttcg
//
LOCUS       DASWNJ010001793         1533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_343407, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001793 DASWNJ010000000
VERSION     DASWNJ010001793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343407"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VGB85_29995"
     CDS             <1..883
                     /locus_tag="VGB85_29995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138369.1"
                     /translation="SALRGPQRAAALLTAQTRTRAADRSARVTLDGLDDDASFAAAGT
                     QTAVRLEGTFKARTPGTYLVVARWLDDHGKPGPVVDATCIRFDVPRRELWASVTIAPD
                     LTMLTPIRDYSAVHLRTQLGYTWYLARPWLGVGVAGGYTFTRYVSSAGLPSWQDFQAD
                     PATGAQPLKWRRHALVVGPLVELRSRRAGLPVELRARMSAGLGVALVDVAGVSTLPNF
                     ATAPGYGPASLRLRSTFDATLELGLGYHAGPLAIAHVLTLGAVAINDMFSATRAVTAT
                     SGAGLFAGIGLILGGTP"
     gene            880..>1533
                     /locus_tag="VGB85_30000"
     CDS             880..>1533
                     /locus_tag="VGB85_30000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138370.1"
                     /translation="MTRVRRCLALLLAGLASACAGLNTGEDDGCVETCAAAYTCGFLP
                     SILGYAADAETAVTDCERRCEQSPREDPAVALILACMQGAAEPKNDFMPWCLDTDAPA
                     YAAGLACATAAACLLEAAQGVQLVSGVSLAVSMISFADYEAVFGEGSVSQLYADDMGE
                     VHSCSPALCGAADCMRSQRLDLPCDDIMCRSTGTQTVKACDQLQVSALDILVAERGVS
                     "
BASE COUNT          189 a          652 c          480 g          212 t
ORIGIN      
        1 cagcgcgctg cggggccccc agcgcgccgc ggccctgctg accgcacaga cccgcacgcg
       61 cgccgccgac cgctccgcac gcgtcacgct cgacggcctc gacgacgacg ccagcttcgc
      121 cgccgccggc acccaaaccg ccgtgcgcct cgagggcacc ttcaaggcgc ggacccccgg
      181 cacctacctc gtcgtcgcgc gctggctcga cgaccacggc aagcccgggc ccgtcgtcga
      241 cgccacctgc atccgcttcg acgtcccgcg ccgggagctg tgggccagcg tcacgatcgc
      301 ccccgacctg acgatgctga caccgatccg ggactacagc gccgtgcacc tgcggaccca
      361 gctcggctac acctggtacc tcgcgcggcc gtggctcggc gtcggtgtcg ccggcggata
      421 tacctttacg cgctatgtca gcagcgccgg cctgccctcc tggcaggact tccaggccga
      481 ccccgccacg ggcgcgcagc cgctgaagtg gcggcgccac gccctcgtgg tcggcccgct
      541 cgtcgagctg cgatcgcgcc gcgccggcct gccggtcgag ctccgcgccc gcatgtccgc
      601 cggactcggc gtcgccctcg tcgacgtagc cggcgtctcc acgctgccca acttcgccac
      661 cgcgccgggc tacggccccg cgtcgctgcg cctgcgatcg accttcgacg cgaccctcga
      721 gctcggcctc ggatatcacg ccggcccgct ggcgatcgcg cacgtgctca cgctcggcgc
      781 ggtcgccatc aacgacatgt tcagcgcgac ccgggccgtc accgcgacct ccggcgccgg
      841 cctgttcgcc ggcatcggcc tgatcctcgg agggacccca tgacccgcgt ccgtcgttgc
      901 ctcgcgctgc tgctcgccgg cctcgcctcc gcctgcgccg gcctcaacac cggcgaggac
      961 gacggctgcg tcgagacctg cgccgccgcc tacacctgcg gcttcttgcc gtcgatcctc
     1021 ggctacgccg ccgacgccga gaccgccgtc accgactgcg agcgccgctg cgagcaatct
     1081 ccgcgcgagg acccggccgt cgcgctcatc ctcgcgtgca tgcaaggcgc cgccgagcca
     1141 aagaacgact tcatgccctg gtgcctcgac accgacgccc ccgcctacgc cgccggcctc
     1201 gcctgcgcca ccgcggccgc ctgcctgctc gaggcggcgc agggcgtgca gctggtctcg
     1261 ggcgtctccc tcgccgtctc gatgatctcc ttcgccgact acgaggccgt gttcggcgag
     1321 ggctccgtgt cgcagctcta cgccgacgac atgggcgagg tgcacagctg ctcgcccgcg
     1381 ctgtgcggcg ccgccgactg catgcgcagc cagaggctcg acctcccctg cgacgacatc
     1441 atgtgccgca gcaccggcac ccagaccgtc aaggcctgcg accagctcca ggtcagcgcc
     1501 ctcgacatcc tcgtcgccga gcgtggcgtc tcg
//
LOCUS       DASWNJ010001794         2107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_136585, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001794 DASWNJ010000000
VERSION     DASWNJ010001794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136585"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1506)
                     /locus_tag="VGB85_30005"
     CDS             complement(<1..1506)
                     /locus_tag="VGB85_30005"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0138371.1"
                     /translation="MLDLGKLPSLGAALRDATISFKSREALIEADRHRENGRWTYAEL
                     RAAADGFCAALQGQGFAPGDRCAILMQNQAKWLIGATGALWAGATLVPLDYKLTAPEQ
                     AALVAHAKPRVLLTEWPTWEKLAHESPEPFERVLVLVTEAPADAKLGKAVRFESLAST
                     QPPVYVERSREDIACIVYSSGTGGTPKGCMLSHGNYLAQAEVLGRMYPMTRGERYFSV
                     LPTNHAIDFMCGFLIPLMMGAAVVHQRTLRPAYLGATMQRYGITHMALVPTILKTLEK
                     KIRERLDDLPKWQRIVVDNLIDLNNFATRREPNQKLSSSLLRPIHDQFGGKLKFMFCG
                     GAFVDRDVAEFLNSLGLPVAIGYGLTEAGTVLTVNDLKPFRGDSVGKPVPGVELVLRD
                     QNDAGVGEVWVRGPTVMQGYLDEPELTREAIVDGWLRTGDLGTIDAAGHLKLVGRAKN
                     MIVTEGGKNVYPEDIESAFSELADVEEYSVFAANYLWPEKQLGGDQLVLVAR"
     gene            complement(1506..1742)
                     /locus_tag="VGB85_30010"
     CDS             complement(1506..1742)
                     /locus_tag="VGB85_30010"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY0138372.1"
                     /translation="MAADTLVTLIKLAAKRFGADATQLRPEDDLFARLGIDSFQAVEL
                     ISDLEAEFDIELPDYELQGVKTFEALAQKIDKRR"
     gene            complement(1754..>2107)
                     /locus_tag="VGB85_30015"
     CDS             complement(1754..>2107)
                     /locus_tag="VGB85_30015"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY0138373.1"
                     /translation="IPGFGGIPRLRRDVGNALIRDLLLSGRSIGARKALAVGLVSQMV
                     IGGEGLTAARALAAQSGKFDAVARRAAKAFIKHLPAEELARERALFQELAQGPALMAA
                     LKRFVESTDLRPYLP"
BASE COUNT          305 a          667 c          790 g          345 t
ORIGIN      
        1 gcgtgccacc agcaccagct gatcgcctcc gagctgcttc tcgggccaga gataattcgc
       61 ggcgaacaca ctgtactcct ctacgtcggc aagctcgctg aacgcgctct cgatgtcctc
      121 ggggtagacg ttcttgccgc cctcggtgac gatcatgttc ttggcgcggc cgacgagctt
      181 gaggtgaccg gcggcgtcga tcgtgccgag gtcgccggtg cgcagccagc cgtcgacgat
      241 cgcctcgcgc gtcagctcgg gctcgtcgag gtagccctgc atgacggtcg ggccgcggac
      301 ccacacctcg ccgacgccgg cgtcgttttg atcgcgcagc acgagctcga cgccggggac
      361 cggcttgccg acgctgtcgc cgcgaaacgg cttgaggtcg ttgacggtca gcacggtgcc
      421 ggcctcggtc aggccgtagc cgatcgcgac cggcaggccg aggctgttga ggaactcggc
      481 gacgtcgcgg tcgacgaagg cgccgccgca gaacatgaac ttgagcttgc cgccgaactg
      541 gtcgtggatc gggcgcagca gcgagctcga gagcttttgg ttgggctcgc ggcgggtcgc
      601 gaagttattg aggtcgatca ggttgtcgac gacgatccgc tgccacttgg gcaggtcgtc
      661 gagacgctcg cggatctttt tttcaagcgt cttgaggatc gtcggcacca gcgccatgtg
      721 ggtgatgccg tagcgctgca tcgtcgcgcc gaggtacgcg ggccgcagcg tgcgctggtg
      781 aaccaccgcg gcgcccatca tcagcgggat caagaagccg cacatgaagt cgatcgcgtg
      841 gttggtcggc agaaccgaga agtaacgctc gccgcgggtc atcgggtaca tgcgcccgag
      901 cacctcggcc tgcgcgaggt agttcccgtg cgagagcatg cagcccttgg gcgtgccgcc
      961 ggtgcccgag gagtagacga tgcaggcgat gtcctcgcgg ctgcgctcga cgtacaccgg
     1021 cggctgggta gatgcgagcg actcgaagcg aacggccttg ccgagcttgg cgtcggcggg
     1081 cgcctccgtg acgagcacga gcacgcgctc gaacggctcg ggggactcgt gcgcgagctt
     1141 ctcccacgtc ggccactcgg tgagcagcac gcggggcttg gcgtgggcga cgagggcggc
     1201 ctgctccggc gctgtcagct tgtagtcgag cgggacgagg gtcgcgccgg cccacagcgc
     1261 accggtggcg ccgatcagcc acttggcctg gttctgcatg aggatcgcgc agcgatcacc
     1321 aggcgcgaag ccctggcctt gcagcgcggc gcagaagccg tcggctgcgg cgcgcagctc
     1381 ggcgtaggtc cagcgcccgt tctcgcggtg gcggtcggcc tcgatcaggg cctcgcggct
     1441 cttgaaggag atcgtcgcgt cgcgcagggc ggcgcccagg ctggggagct tgccgaggtc
     1501 gagcattagc gcctcttgtc gatcttctgg gccagcgcct cgaaggtctt gacgccctgc
     1561 agctcgtagt cggggagctc gatgtcgaac tcggcctcga ggtccgagat cagctcgacg
     1621 gcctggaagc tgtcgatgcc gaggcgggcg aacaggtcgt cctcggggcg cagctgcgtc
     1681 gcgtcggcgc cgaagcgctt cgcggccaat ttgatgaggg tgacgagggt gtctgcggcc
     1741 atggctgtct cttttagggc aggtaggggc gaaggtcggt gctctcgacg aagcgcttga
     1801 gcgcggccat cagcgcgggg ccctgcgcga gctcctggaa cagggcgcgc tcgcgggcca
     1861 gctcctccgc gggcaagtgc ttgatgaagg ccttggcggc gcgccgggcg acagcgtcga
     1921 acttgccgga ctgggccgcg agggcgcggg cggcggtgag gccctcgccg ccgatgacca
     1981 tctggctgac gaggccgacg gcgagcgcct tgcgggcgcc gatgctgcgg ccgctgagca
     2041 gcaggtcgcg gatcagggcg ttgccgacgt cgcggcgcag gcgggggatg ccgccgaagc
     2101 ccgggat
//
LOCUS       DASWNJ010001795         1620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_668425, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001795 DASWNJ010000000
VERSION     DASWNJ010001795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668425"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VGB85_30020"
     CDS             complement(<1..261)
                     /locus_tag="VGB85_30020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138374.1"
                     /translation="MSEDGSIPASETSNWTVPRLNNFRQNVQPEGEFAAPDLVVSGTA
                     QCFDGLDVAARVRNIGQASVPAGVTVGFYQGDPAEGGTLLGTG"
     gene            complement(568..>1620)
                     /locus_tag="VGB85_30025"
     CDS             complement(568..>1620)
                     /locus_tag="VGB85_30025"
                     /inference="COORDINATES: protein motif:HMM:NF017142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138375.1"
                     /translation="QQRLRPVLADVAVAGEAVDAAGVAEHDGLPGAGDVVDERGRQPL
                     GGLNRLAQVELDEVRVVGDRRLDEQPAVALEDQRAALGACVGAEKWFTEGFTEFYTWR
                     LLLRAGLYTLEEFIDDWNAALLEYDTSPVRGEPNARIVEDYWNDKAVGRLPYRRGPLL
                     AAIWDQRLRAATGGARDLDDVVLAMRAQAREGNQLARDAASSFAPTYHALGGPDLGPD
                     LAKYVEQGAAIDLPADVFGPCIEVTRKPRRSFDRGWDADATTKAGNVITGLRKDSPAH
                     RAGLRDGMKIVERVAGEPGDSTVEYVLKVLDGERERKIRFLPGGDQELVVQRLVLVKK
                     LSTAQREACTRTLAGL"
BASE COUNT          254 a          548 c          562 g          256 t
ORIGIN      
        1 gccggtgccg aggagggtgc cgccctcggc ggggtcacct tgataaaagc cgacggtgac
       61 gccggcgggc acggaggcct ggccgatgtt gcgcacgcgc gcggcgacgt cgaggccgtc
      121 gaagcactgg gcggtgccgg agacgacgag gtcgggagcc gcaaactcgc cttctggttg
      181 cacgttctgg cggaagttgt tgaggcgagg cacagtccag ttgctggtct cggacgcggg
      241 gatcgagcca tcttcggaca cgttcgtcac atgatgtacg cgagtagcta gcgtatctgg
      301 gagctggaat tcgaggcttt gtatcgaatt tctcgaaatc gagacaagat cgcgacgaaa
      361 tttcgagacg acagggagat cacgccgtcg cgcattcaga cgttcgacgg caggctatca
      421 cccctgcggc acaagccctg tggccgccgg ccgctgggcc gacgagccag ccacaacgaa
      481 gccagcggtg aggggacgct cgcgcggatg cagggcgggc gccatctccg catgctcaag
      541 gtcagcgcga agttcccggt gcgtcgctca caggcccgcg agcgtgcggg tgcaggcctc
      601 gcgctgcgcg gtcgacaatt ttttcacgag cacgagtcgc tgcacgacga gctcctggtc
      661 gccgccgggc aagaagcgga tctttcgctc gcgctcgccg tcgaggacct tcaggacgta
      721 ctcgacggtc gagtcgccgg gctcgccggc gacccgctcg acgatcttca tgccgtcgcg
      781 caggccggcg cggtgcgcag gtgagtcctt gcgcagacct gtgatcacgt tgccggcctt
      841 tgtcgtggcg tccgcgtccc agccgcggtc gaagctcctg cgcggcttcc tcgtgacctc
      901 gatgcacggg ccgaacacat cagcgggaag atcgatcgct gcaccctgtt cgacgtactt
      961 cgccaggtcg ggaccgaggt ccggaccgcc gagagcgtga tacgtgggcg cgaagctgga
     1021 cgcggcgtcc cgcgccaatt ggttgccctc gcgggcttgc gcgcgcatcg ccaggaccac
     1081 atcgtcgagg tctcgcgcgc cgccggtggc cgcgcgcagg cgctggtccc agatcgcggc
     1141 cagcagcgga ccgcggcggt agggcaggcg gccgaccgcc ttgtcgttcc aatagtcctc
     1201 gacgatgcgc gcgttcggct cgccgcgcac gggcgaggtg tcatactcga gcagcgccgc
     1261 gttccagtcg tcgatgaact cctcgagcgt gtacaggccg gcgcgtagga gcaggcgcca
     1321 ggtgtagaac tccgtgaagc cctccgtgaa ccacttctcg gcgcccacgc aggcgcccag
     1381 cgcggctcgc tgatcctcga gcgcgacggc cggctgctcg tcgaggcgac gatcacccac
     1441 gacccggacc tcgtcgagct cgacctgcgc gagccggttg agacctccga gcggctggcg
     1501 acctcgctcg tccactacgt cgcccgcacc cgggagaccg tcgtgctcgg cgacgccagc
     1561 cgcgtcgacc gcttcgccgg cgaccgctac atcagccagc accggccgaa gtcgctgctg
//
LOCUS       DASWNJ010001796         1750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_414945, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001796 DASWNJ010000000
VERSION     DASWNJ010001796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414945"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1609
                     /locus_tag="VGB85_30030"
     CDS             <1..1609
                     /locus_tag="VGB85_30030"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138376.1"
                     /translation="KSSGTSYVVLGKASSDPVFLDAVAVGEGGFAIYGEAAQQFSGHS
                     VKSAGDVNGDGLADIIVGAYGAEPNGVLSGRAYVVFGRQAGKPTELDAIAAGLGGGFV
                     VNGEAELDFAGSAVAGAGDVDGDGLDDIIVGAYGSDINGDTAGRAYVVFGKASETPVD
                     LSAIAAGLGGFAIDGELPFDQAGAAVAGAGDVNGDGLADVIVGAPLADAQSDDAGRSY
                     VVFGKSSPTAVQLAAVSQGIGGFAIDGQQVRDYSGFSVDAAGDVNGDGLDDVILGAYG
                     ANPSGDASGRSYVVFGRTDGQLIALNAIANGDGGFALDGENGDDYSGFAVGAGGDADG
                     DGFTDVIVGAFGSDAKGDGAGRSYVVFGGDYSNVARSVGGQGPDNLAGTDKPEIFVSG
                     RGDDTITGQGGADIFYCGAGEDTVRLGDLGFRRLDGGAGEDTLQLMGAGLTLDLTMMP
                     DNALVSVEVIDLADGEHTVVLARRDLLALARTTHKLTITGSKGVVEADLAGAGFLDGG
                     EIGGYQVYSDGVTTLRVASTLAKNVTL"
BASE COUNT          252 a          600 c          664 g          234 t
ORIGIN      
        1 caagtcgtcg ggcacctcgt acgtggtgct cggcaaggcc agcagcgacc cggtgttcct
       61 cgacgcggtc gcggtcggcg agggcggctt tgcgatctac ggcgaggcgg cgcagcagtt
      121 ctccgggcac agcgtgaaga gtgccggcga cgtcaacggc gacggcctcg cggacatcat
      181 cgtcggcgcg tacggagcgg agcccaacgg cgtgctgtcg ggccgcgcct acgtcgtgtt
      241 cgggcgccag gccggcaagc cgaccgagct cgacgcgatc gcagcggggc tcggcggcgg
      301 cttcgtggtc aacggcgagg ccgagctcga cttcgccggc agcgcggtcg cgggcgcggg
      361 cgacgtcgac ggcgacggcc tcgacgacat catcgtcggc gcgtacgggt ccgacatcaa
      421 cggcgacacc gccggccggg cctacgtggt gttcggcaag gcgagcgaga caccggtcga
      481 cctgagcgcg atcgcagcgg gcctcggcgg gtttgcgatc gacggcgagc tccccttcga
      541 ccaggccggg gcggccgtcg cgggcgcggg cgacgtcaac ggcgacggcc tcgcggatgt
      601 catcgtcggc gcgccgctgg ccgacgccca gagcgacgac gccggccgca gctacgtcgt
      661 gttcggcaag agcagcccga cggccgtgca gctcgccgcg gtctcgcagg gcatcggtgg
      721 gtttgcgatc gacgggcagc aggtgcgcga ctacagcggc ttttcggtcg acgcggcggg
      781 cgacgtcaac ggcgacggac tcgacgacgt catcctcggc gcgtacgggg ccaacccctc
      841 gggtgacgcc tcgggtcgca gctacgtggt cttcgggcgc acggacggcc agctgatcgc
      901 gctcaacgcg atcgccaacg gcgacggcgg gttcgcgctc gacggcgaga acggcgacga
      961 ctactccggc ttcgcggtcg gcgcgggcgg ggacgcggac ggcgacggct tcacggacgt
     1021 catcgtcggc gcgttcggca gcgacgccaa gggcgacggg gccgggcgca gctacgtcgt
     1081 gttcggcggc gactacagca acgtcgcgcg ctcggtcggc ggccagggcc cggacaacct
     1141 cgccggcacc gacaagccgg agatcttcgt cagcggtcgc ggcgacgaca cgatcaccgg
     1201 gcagggcggc gccgacattt tttattgtgg ggccggcgag gacaccgtgc gcctcggcga
     1261 cctcgggttc cgtcgcctcg acggcggcgc cggcgaggac acgctgcagc tgatgggcgc
     1321 cggtcttacg ctcgacctga ccatgatgcc ggacaacgcg ctcgtcagcg tcgaggtgat
     1381 cgacctcgcc gacggcgagc acaccgtcgt gctggcgcgg cgcgacctgc tcgcgctggc
     1441 gcggacgacc cacaagctga cgatcacggg cagcaagggc gtcgtcgagg ccgacctcgc
     1501 gggcgcgggc ttcctcgacg gcggcgagat cggcggctat caggtctaca gcgacggcgt
     1561 gacgaccctg cgggtcgcca gcacgctggc gaagaacgtc acgctctgac atgttgggcc
     1621 gatgccgggc gacgcgcgtc tggaggtcct cagctcccgt ggcctgcccg gctggctcgg
     1681 cgaacacgcg ctgagcctcg cgttcacgac ctaccaggcc ggcaagctgt tcctgctcgg
     1741 cgtgcaaacg
//
LOCUS       DASWNJ010001797         6041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_668429, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001797 DASWNJ010000000
VERSION     DASWNJ010001797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668429"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="VGB85_30035"
     CDS             complement(<1..1214)
                     /locus_tag="VGB85_30035"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY0138377.1"
                     /translation="MSSPEPPQGSSDPPTGAGAREAGAASPVIFAGGGEMGALIRALD
                     WTRTPLGAPATWPQSLRTAVSVMLEARFPMYLAWGPRFVQFYNDGYRPILGSTKHPAA
                     LGGLGQDTFAESWHVIGPMFAGVMRGTAVGADDWMLPLDRHGYLEECYFTFSYSPIRD
                     ESGEVGGVLVTVSETTRRVLGERRLRTLRDLAARAGAAQREDDAWRGAAEALASNDLD
                     LPFLLLYRAPDAPPEVGRAQPELVASSGWDPAEAAGAGPLWPIAEVMATGVAQLVPDL
                     ERLGARAPTGPWTEAPRTALVLPITRPGAGEPTGALVAGVSARRVLDDDYRGFLQLVA
                     DQIGSAVGNARAFVAEQQRTATLAALDHAKTAFFSNVSHEFRTPLTLIMGPVEDSLAD
                     RDEPLSPVQRER"
     gene            complement(1375..2724)
                     /locus_tag="VGB85_30040"
     CDS             complement(1375..2724)
                     /locus_tag="VGB85_30040"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HEY0138378.1"
                     /translation="MLTARFIRLLPLAAIGLACGPATPAKPEGKVTEPAAPAPVVAPA
                     SETSKPASEVAKVQPAAEVQPLDADTGARVAESINAFAVDLHHALADQPGNLFVSPAS
                     IAVAFAMTHAGARGETEQQIARVFHFGDDAAKTREGFAAALAAWAVPQENLELSVANR
                     LFGEKTVAFEPAFIDLTRTLFAAPLELTDFKGAPDPAREHINGWVSAHTHDKITNLVP
                     VGAISASTRLVLVNAVYFKAGWREPFSEALTKNGAFHVASGKKTVRLMTRTDELAANV
                     VASAGLKLVELPYQGTFSLVVVLPDAKDGLAAVEKAMTAEALREWISGMRKQPVAVKL
                     PSFEIVPTTSLQLAATLGRMGMPMAFDAQAADFTGMAPKSEQIVLSEAVHRAFVAVDE
                     KGTEAAAATAVVMFRGGGMKPSGEPLDFTVDHPFLFMIRDTKTGAILFMGRFTDPQP"
     gene            complement(2864..3418)
                     /locus_tag="VGB85_30045"
     CDS             complement(2864..3418)
                     /locus_tag="VGB85_30045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138379.1"
                     /translation="MRSSDIVLILGALASGALACAARSPGEATEIPAAASREAAESAC
                     KHELGRCGGHKPGDGACGSAAPDTADAEPPTPTPLEDVVLAPGAFAEIDLEMAAGATT
                     EVTFQARGGSLEWNVHSHQGDAVTTHAEGTGATGKVQFAAPSAGLYSYMWKNTGGATV
                     LLTARLVAQGTVRVRSIHPTPAGT"
     gene            3514..3954
                     /locus_tag="VGB85_30050"
     CDS             3514..3954
                     /locus_tag="VGB85_30050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138380.1"
                     /translation="MLTLTSTLTAALVLASLQPVAEGPAPEPASAPLSPGGSLAPDLD
                     RERRLATWTAERRRLRLHTGLSGAFAGVMLVTGVVLMVAPDGCDNMNCGQNLGRLFAG
                     IGLTALSIIPISTGIYWGVRLHRHGKVRPTAVLRPGPGGFALQF"
     gene            complement(3967..4761)
                     /locus_tag="VGB85_30055"
     CDS             complement(3967..4761)
                     /locus_tag="VGB85_30055"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY0138381.1"
                     /translation="MTIAVPGFTCSNITIAGAARDVYRRGEGPAVVVLSEIPGITPAV
                     AGFAARVADAGFTVFMPQLFGTPMRPVEAGALLDAVSHVCISREFRMLWADRSSPVID
                     WLRGLARDAHAECGGPGVGVVGMCMTGNFGLAMMLGAPVIAPVLAQPSLPLPIGARRR
                     PALHASPAEIAAAHDKIDNAGARILGLRFAGDPLCPAARFEHLRAEFGAAFEAIEIAD
                     EHANPAGPRPAHSVLTNHLIDADGQPTRAALDRTIAFLREQLHAPA"
     gene            complement(4783..5886)
                     /locus_tag="VGB85_30060"
     CDS             complement(4783..5886)
                     /locus_tag="VGB85_30060"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0138382.1"
                     /translation="MTRVRPGISLLLLLACTLVACAGPRRARTRMVEGWVTATPDEVD
                     MNGALLRAAVARLDGERRHGIDSMLVVRRGRLVAERYWNGHDMGTLHDLRSATKSVTS
                     LLVGIALDRGLLGDVHDPALPYLARAYPELASAGDRPITLEHLLTMRSGLACDDRDRR
                     SPGHEDRMYRSRDWTRFFLELPRTHPPGERTVYCTGGVVALGRIVAEAAGQSIAEFAG
                     EALFAPIGILAYSWRSFDGRRQIDTGGHLRLRPRAMAKIGQLVLQRGVWDQGEVVSGA
                     WIDASTAVHTRFDADRRPYGYLWWQVQASLGTRRVDLIHASGNGGQYIFIAPSLDLVV
                     VFTGSNYNSPRAERPFAILAEYILPAAVADTGP"
BASE COUNT          839 a         2102 c         2275 g          825 t
ORIGIN      
        1 tggcgctcgc gctggaccgg tgagagcggc tcgtcgcggt cggcgaggct gtcctcgacc
       61 gggcccatga tcagcgtcag cggggtgcga aactcgtggc tcacgttgct gaagaacgcc
      121 gtcttggcgt ggtcgagcgc cgcgagggtc gcggtgcgct gctgctccgc cacgaaggcg
      181 cgcgcgttgc cgacggccga gccgatctga tcggcgacga gctggaggaa gccgcgataa
      241 tcgtcgtcga gcacccgtcg cgcgctgacg cctgcgacga gggccccggt cggctcgccc
      301 gcgccggggc gcgtgatcgg caggaccagc gccgtgcgtg gcgcctcggt ccacggaccc
      361 gtcggcgcgc gggcaccgag gcgctcgagg tccggcacga gctgcgcgac accggtcgcc
      421 atgacctccg cgatcggcca caggggcccc gcccccgccg cctctgccgg gtcccagccg
      481 gacgacgcga cgagctcggg ctgcgcgcgc ccgacctccg gtggcgcgtc cggggcgcga
      541 tacagcagca agaacggcag atcgaggtcg ttgctcgcca gcgcctcggc ggcgccgcgc
      601 caggcgtcgt cctctcgttg ggccgcgccg gcacgcgcgg cgaggtcacg cagcgtccgc
      661 agccggcgct ccccgagcac gcgccgggtg gtctcgctga cggtgacgag caccccgccg
      721 acctcgccgc tctcgtcccg gatcgggctg taggagaacg tgaagtagca ctcctcgagg
      781 tagccgtggc ggtcgagcgg caacatccag tcgtcggcac cgacggcggt gcctcgcatc
      841 acccccgcga acatcgggcc gatgacgtgc cagctctccg cgaaggtgtc ctggccgagc
      901 ccgccgagcg ccgcggggtg cttggtcgac ccgaggatcg ggcgatagcc gtcgttgtag
      961 aactgcacga agcgcggccc ccaggcgagg tacatcggga agcgcgcctc gagcatgacg
     1021 ctgaccgccg tccgcagcga ctgcggccac gtcgcgggcg caccgagcgg ggtcctggtc
     1081 caatccaggg cacgaatcag cgcacccatc tcgccgccgc ccgcgaagat gaccggcgag
     1141 gccgcgccgg cctcccgcgc gcccgcgccc gtcggcggat cgctcgaacc ctgcggaggc
     1201 tccggactgc tcatcgactg tagctaacac accgacgccg gatggcccag gaccggccgt
     1261 cgacgccgcg agcagcagca cggcgaacgc ggcggtcacg gtgacgggac gcggctttta
     1321 ctgcacactg ccgccgagct acgcggcgaa tgatctgccg gtgacgcgcg gccgtcacgg
     1381 ctgcgggtcg gtgaagcggc ccatgaacag gatcgccccg gtcttcgtgt cgcggatcat
     1441 gaacaggaag gggtgatcga cggtgaagtc gagcggctcg ccggagggct tcataccgcc
     1501 gccacggaac atgaccaccg cggtggcggc ggccgcctcg gtgcccttct cgtcgacggc
     1561 gacgaatgcc cggtgcacgg cctcggagag gacgatctgc tcggacttcg gggccatgcc
     1621 ggtgaagtcg gcggcctgcg cgtcgaaggc catcggcatg cccatacgcc cgagggtcgc
     1681 ggcgagctgc aggctcgtgg tcggcacgat ctcgaagctc ggcagcttga ccgcgaccgg
     1741 ctgcttgcgc atcccggaga tccactcgcg cagggcctcg gccgtcatcg ccttctcgac
     1801 cgcggcgagc ccgtccttgg cgtcgggcag cacgacgacg agcgagaacg tgccctgata
     1861 cggcagctcg acgagcttga ggccagcgct ggcgacgacg ttggcggcga gctcgtcggt
     1921 gcgggtcatc aggcggacgg tctttttgcc ggacgcgacg tggaaggcgc cgttcttggt
     1981 cagcgcctcg ctaaacggct cgcgccagcc ggccttgaag tacacggcgt tgacgaggac
     2041 cagccgggtg ctcgcggaga tcgctccgac cggcacgagg ttcgtgatct tgtcgtgggt
     2101 gtgggcggag acccagccgt tgatgtgctc gcgggcgggg tcgggggcgc ccttgaagtc
     2161 ggtcagctcg agcggggcgg cgaacagcgt gcgggtgagg tcgatgaagg ccggctcgaa
     2221 ggcgacggtc ttctcgccga acaggcggtt ggcgaccgag agctcgaggt tctcctgcgg
     2281 cacggcccag gcggcgagcg ccgccgcgaa gccctcgcgc gtcttcgcgg cgtcgtcccc
     2341 gaagtggaac acgcgcgcga tctgctgctc ggtctcgccg cgggcgccgg cgtgggtcat
     2401 cgcgaacgcg acggcgatgc tagccggcga cacgaacagg ttccccggct ggtcggcgag
     2461 cgcgtggtgc aggtcgaccg cgaaggcgtt gatgctctcg gccacccgcg ctccggtgtc
     2521 cgcgtcgagc ggctggacct ccgccgccgg ctgtaccttg gcgacctcgg acgcgggctt
     2581 cgacgtctcg ctcgccggtg cgacgacggg cgcgggcgct gccggttcgg tcaccttgcc
     2641 ctcgggcttg gcgggcgtgg ccggaccaca ggcgaggccg atcgccgcga gcgggaggag
     2701 acggatgaag cgagcggtga gcacagtgcc atataccgtg gctcacgcgc ggtgaacagc
     2761 acctccggcg cggagtattc acattccggc gcgagaatgc acgcgcgacg cgagcggcca
     2821 cgacgtgggt cggcgtgtat gacgatagtg ctgccgtgta aggctacgtg cccgccggtg
     2881 tcgggtggat cgaccgcacg cggacggtgc cctgcgctac gaggcgtgct gtcaacagca
     2941 cggttgcgcc accagtgttt ttccacatgt acgaatagag gccggcagac ggcgccgcga
     3001 actgcacctt gccggtcgcg ccggtgccct cggcgtgggt cgtgacggcg tcaccctggt
     3061 ggctgtgcac gttccactcg agcgagccgc cgcgcgcctg aaacgtcacc tcggtcgtcg
     3121 cgcccgcggc catctcgagg tcgatctcgg cgaacgctcc cggcgcgagc acgacgtcct
     3181 ccagcggcgt cggcgtcggg ggctccgcgt cggcggtgtc cggggccgcc gaaccacacg
     3241 cgccgtcgcc cggcttgtga ccgccgcagc ggccgagctc gtgcttgcac gcgctctcgg
     3301 ccgcctcgcg cgaagcggcc gcggggatct cggtcgcctc gccgggcgac cgggcagcac
     3361 acgcgagcgc gccgctggcg agcgcgccga ggatgaggac gatgtcggag gatcgcatgg
     3421 cgtgcgcgca ggatatcctc gatcgccgag cggtgtggga ccgcgcggcc acgtagtgct
     3481 ggaccggtcg cgggcggggg tccacgatga cccatgctta cgcttacgtc gaccttgacc
     3541 gccgcgctcg tcctcgccag ccttcagccc gtcgccgagg gtcccgcgcc ggagcccgcg
     3601 agcgcaccgc tgtcgcccgg cgggtcgctc gcgcccgacc tcgaccgcga gcggcgactc
     3661 gccacgtgga ccgccgagcg ccggcggctg cggctgcaca ccgggctgtc gggcgcgttc
     3721 gcgggggtca tgctggtgac cggcgtcgtg ctgatggtcg ccccggatgg ttgcgacaac
     3781 atgaattgcg ggcagaacct cggccgtctg ttcgcgggca tcggtctgac cgcgctgtcg
     3841 atcatcccga tcagcaccgg catctactgg ggggtccgcc tgcaccggca tggcaaagtg
     3901 cgcccgaccg cggtgctgcg cccggggccc ggcggcttcg cgttgcagtt ctgagcgcag
     3961 gcctgatcag gccggcgcgt gcagctgctc ccgcaagaac gcgatcgtgc ggtcgagcgc
     4021 ggcgcgggtc ggctggccgt cggcgtcgat caggtgattg gtgagcacgg agtgcgccgg
     4081 gcgcgggccg gccgggttgg cgtgctcgtc ggcgatctcg atcgcctcga aggccgcgcc
     4141 gaactccgcg cgcaggtgct cgaagcgggc cgcggggcac agcggatcgc cggcgaagcg
     4201 cagcccgagg atccgcgcac cggcgttgtc gatcttgtcg tgggccgccg cgatctcggc
     4261 cggcgaggcg tgcagcgcgg gcctgcggcg ggcgccgatc ggcaacggca gggacggctg
     4321 cgccagcacc ggggcgatca ccggagcgcc gagcatcatc gccaggccga agttgccggt
     4381 catgcacatg ccgacgacgc cgacccccgg gccgccgcac tcggcgtgcg cgtcgcgggc
     4441 gagcccccgc agccagtcga tcaccgggct cgagcggtcg gcccacagca tgcgaaactc
     4501 acggctgatg cacacgtggc tgaccgcgtc gagcagcgcg ccggcctcga ccgggcgcat
     4561 cggcgtgccg aacagctgcg gcatgaacac ggtgaagccg gcgtcggcga cccgcgcggc
     4621 gaagccggcg accgcgggcg tgatgccggg gatctccgag agcacgacga ccgccgggcc
     4681 ctcgccgcgg cgatacacgt cacgcgcggc gccggcgatc gtgatgttcg agcaggtgaa
     4741 gccggggacc gcgatcgtca tgcgggcaaa atgtaaccgt tttcacgggc cggtgtccgc
     4801 gaccgcggcg ggcaggatgt actcggcgag gatcgcaaac gggcgctcgg ccctcggcga
     4861 attgtagttg ctgccggtga acacgacgac gaggtcgagc gacggcgcga tgaagatgta
     4921 ctggccgccg ttgcccgagg cgtggatcag gtcgacgcgg cgcgtcccga ggctcgcctg
     4981 cacctgccac cacaggtagc cgtagggtcg ccggtcggcg tcgaagcggg tgtgcacggc
     5041 cgtcgaggcg tcgatccagg cgcccgagac gacctcgccc tggtcccaga cgccgcgctg
     5101 cagcacgagc tggccgatct tcgccatcgc ccgcgggcgc aggcgcaggt gcccgccggt
     5161 gtcgatctgg cgccggccgt cgaagctgcg ccagctgtac gcgaggatcc cgatcggggc
     5221 gaacagggcc tcgcccgcga attcggcgat cgactggccc gcggcctcgg cgacgatccg
     5281 cccgagcgcc accacgccgc cggtgcagta gacggtgcgc tcgccgggcg ggtgcgtgcg
     5341 cggcagctcg aggaagaagc gggtccagtc gcgcgaccgg tacatgcggt cctcgtggcc
     5401 cggcgagcgg cggtcgcggt cgtcgcacgc gaggccgctg cgcatcgtca gcaggtgctc
     5461 gagggtgatc ggtcgatcgc cggcggacgc cagctccggg tacgcgcggg cgaggtacgg
     5521 gagggccggg tcgtgcacgt cgccgagcag gccgcggtcg agggcgatgc cgacgagcag
     5581 ggaggtgacg ctcttggtcg ccgagcgcag gtcgtgcagc gtgcccatgt cgtggccgtt
     5641 ccagtagcgc tcggcgacca gccggccgcg ccggaccacc agcatgctgt cgatgccgtg
     5701 ccggcgctcg ccgtcgaggc gcgcgaccgc ggcccgcagc agcgcgccgt tcatgtcgac
     5761 ctcgtccggc gtcgcggtga cccagccctc gaccatgcgc gtgcgagcgc gccgcggccc
     5821 ggcgcaggcg accagcgtgc acgcgaggag cagcagcagc gatatgcccg gacgaactcg
     5881 cgtcacttgc gagcctgccc gaagcggtgg gcgaacactc cggcgatcgc gaagtagacc
     5941 gcgtgctggt cgcggccgag ctgcggctcg tcctcgagga tcgccgcgcc gcgataaaaa
     6001 tagccctggg ccagggtcgt atgatcgtag cggtattcga g
//
LOCUS       DASWNJ010001798         2504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_491634, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001798 DASWNJ010000000
VERSION     DASWNJ010001798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491634"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VGB85_30065"
     CDS             <1..444
                     /locus_tag="VGB85_30065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138383.1"
                     /translation="PAPAPAPAPTPKPATATQATPATPVVKPKPAPKPTPAPEPPGAT
                     EDPPPLVKPGPDARPPLDTAAAAEVVRAATARIRRCAAEQAMVGNFTLQVKVDRQGKA
                     TGVDAAASSRKIPAEALACMRDVIAGESFQASARGGVVSVTLSAK"
     gene            complement(448..957)
                     /locus_tag="VGB85_30070"
     CDS             complement(448..957)
                     /locus_tag="VGB85_30070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138384.1"
                     /translation="MQPRALLGIAAALGVAYATYAWTRPPADLPADLPAGAAEAAIHR
                     PPPAAHDVLDEQDHRAVASALQRAFGPPPEPELKLPPARSRREAEASFEGMMTALEEL
                     ADAGKKLPRARRDQLYRDTNDVFSALSAHLDPNDAADMQLLEDANIRMKAMLGELGVR
                     VPRRLAPAE"
     gene            complement(1033..2250)
                     /locus_tag="VGB85_30075"
     CDS             complement(1033..2250)
                     /locus_tag="VGB85_30075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138385.1"
                     /translation="MRISFLCLGLFVAACGGETSTSESAEGTDSTGVASTTPGSDSTA
                     TDDTPTTDASTTGGGASEAMTGDTTATPSTDPTTTTDTTTTDTTDTGDTGGAVPGAFC
                     APIPACDGAPPMVPGQGPEESGYSRARDMMYVEGDEQWILAKFTKWGFPSDKDVIGST
                     VHVFLDRNCSGEWVELGTTVTTDEGDHASVEGVEDSGGRVYFQIPADQKLELGRHRVH
                     LVDEDEWETADALIDIVPKGAPFFVSDVDGTLTTSENEEAYDFLAGNLPDANPFAAQA
                     LFILANKGYRPMYITARPEWLDRRTREFVEVHNFPRGIIHTTTLYEGANGDAAALYKS
                     GEFALLKQKGLVPSFVFGNKDSDALAFDNAGVQPLDHRFFFQFTDETYGGRRIESYEE
                     LLAEFEALPDLCD"
BASE COUNT          333 a          844 c          974 g          353 t
ORIGIN      
        1 ccggccccgg ccccggcccc ggcaccgacg ccgaagcctg ccaccgcgac gcaggcgacg
       61 ccggcgacgc cggtggtgaa gccgaagccc gcgccgaagc cgacgcccgc gccggagccg
      121 cccggggcga ccgaggaccc gccgccgctg gtgaagccgg ggcccgacgc gcggccgcct
      181 ctggacaccg ccgcggcggc cgaggtggtc cgggccgcga cggcgaggat ccgccgctgc
      241 gccgcggagc aggcgatggt cggcaacttc acgttgcagg tgaaggtcga tcgccagggc
      301 aaggcgaccg gcgtcgacgc ggccgccagc tcgcgcaaga tccccgcgga ggcgctcgct
      361 tgcatgcgcg acgtgatcgc gggtgagagc ttccaggcga gcgcgcgcgg cggtgtggtc
      421 agcgtgacgc tgtcggccaa gtagcggcta ctcggccggc gcgaggcgcc tcggcacccg
      481 gacgccgagc tcgccgagca tcgccttcat gcggatgttg gcgtcctcga gcagctgcat
      541 gtcggcggcg tcgttggggt ccaggtgggc ggacagcgcg gagaacacgt cgttcgtgtc
      601 gcggtagagc tggtcgcgac gggcgcgcgg gagcttcttg ccggcgtcgg cgagctcctc
      661 gagcgcggtc atcatgccct cgaagctcgc ctcggcctcg cggcgggacc gcgccggcgg
      721 gagcttgagc tcgggctcgg gcggcgggcc gaaggcgcgc tggagggcgc tggcgacggc
      781 gcgatgatcc tgctcgtcga gcacgtcgtg ggcggcgggc ggcgggcgat ggatcgcggc
      841 ctccgccgca ccggcgggca ggtccgcggg cagatcggcg gggggccgcg tccacgcgta
      901 ggtcgcgtag gcgacgccga gcgcggcggc gatgccgagg agggcgcggg gctgcatggg
      961 cgtgacggag ggtgacgcac cggcgcgcgc ggggcaagtc ctgcggaccc gaccccgccc
     1021 ggactcgccg gtctagtcgc acaggtcggg cagggcctcg aactcggcga gcagctcctc
     1081 gtagctctcg atcctgcggc cgccgtaggt ctcgtcggtg aactggaaga agaagcggtg
     1141 atcgagcggc tggaccccgg cgttgtcgaa ggcgagggcg tcgctgtcct tgttgccgaa
     1201 cacgaagctc ggcacgaggc ccttttgctt gagcagcgcg aactcgccgg acttgtacag
     1261 cgcggcggcg tcgccgttgg cgccctcgta gagggtggtc gtgtggatga tgccgcgcgg
     1321 gaagttgtgt acctccacga actcgcgggt gcggcggtcg agccactccg ggcgggccgt
     1381 gatgtacatc ggccggtagc ccttgttggc gaggatgaag agcgcctgcg cggcgaaggg
     1441 gttggcgtcc gggaggttgc cggccaaaaa gtcgtaggcc tcctcgttct cggaggtggt
     1501 cagggtgccg tcgacgtcgc tgacgaagaa gggcgcgccc ttgggcacga tgtcgatcag
     1561 ggcgtcggcg gtctcccact cgtcctcgtc gacgaggtgg acgcggtgac gaccgagctc
     1621 gagcttctga tcggcgggga tctggaagta cacgcggccg ccggagtcct cgacgccctc
     1681 gaccgaggcg tggtcaccct cgtcggtggt gacggtggtg ccgagctcga cccactcgcc
     1741 gctgcagttg cggtcgagga acacgtgcac ggtgctgccg atgacgtcct tgtcggacgg
     1801 gaagccccac ttggtgaact tggcgaggat ccactgctcg tcgccctcga cgtacatcat
     1861 gtcgcgcgcg cgcgagtagc cgctctcctc cgggccctga cccgggacca tcggcggggc
     1921 accgtcacac gccgggatcg gggcgcaaaa ggcccccggc accgcgccgc cggtgtcgcc
     1981 ggtgtccgtg gtgtcggtcg tggtcgtgtc ggttgtcgtg gtcgggtcgg tggacggcgt
     2041 cgcggtcgta tcgcccgtca tcgcctccga ggccccgccg ccggtcgtgc tcgcgtcggt
     2101 ggtcggcgtg tcgtccgtcg ccgtggagtc gctgccgggg gtagtggacg ccacgcccgt
     2161 gctgtcggtc ccctccgcgg actcgctggt gctggtttcg ccgccacagg cagcgacgaa
     2221 gaggccgagg caaaggaagg agatccgcat gcgcgcgcat cgtactgcaa gcgaccggct
     2281 ttcaccacga ccgcgaacgc gggcacctgc gcccgttgat gacgaggtgc ttcacgacgc
     2341 gcaggtcact tggcgacgcc gcgcgaccac ggcgacgaac gcggcgaaca gcgcgtcacc
     2401 cggcgcgcgg ggcggcgagc cggtcgcgat ctgccactcc gggtgccact gcacgccgaa
     2461 ggcgaacgag gacgcatgcg ccacgcgcac cgcctccacc agcc
//
LOCUS       DASWNJ010001799         2451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_280131, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001799 DASWNJ010000000
VERSION     DASWNJ010001799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280131"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="VGB85_30080"
     CDS             <1..1051
                     /locus_tag="VGB85_30080"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY0138386.1"
                     /translation="ADGGEVRAGVSLPLWQGRAVDRRRTDLRVTALAQDGAAADLEAR
                     MIELERAAGRAYWAWVGAGLRLQVQRSLLALAEDRDEALRKQIAAGSVPAVEGLDNRR
                     AILDRGARIVAAERALQQAAFELARHLRDANGNLETPAPGRLPDAFPEPPPPAADAIE
                     QTIREAWARRPDLLRLRLQREAAAMELKLARGDRSPKIDLDAYGAKDIGATAPEYAYL
                     RPAEFVASVSIELPLALRGARGKLRRAEATLARIDAELRMARDVIALEIRDAHSALVA
                     AHRRVALAREQLAAAHELEQAERSRFRLGDSTLLLVNLREQAAADAAQQEVDALADYH
                     RASVDLRAAAGLFGP"
     gene            complement(1071..1952)
                     /locus_tag="VGB85_30085"
     CDS             complement(1071..1952)
                     /locus_tag="VGB85_30085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138387.1"
                     /translation="MRPERLAVLAILALAGCVREGSAAEVDATTGDGSTGATTGTTTA
                     VDCEGLSDDLYTRRIAPLLATDRPKTCNTCHLSGIDLEMFVQDTPCQTMACLETKGLV
                     DLADPASSTILRWISRADPASPLIDQRVIDEEYAAFLAWIEATATCGGCYVGDAPCGT
                     PVQHSCEADDPKTDTYEDPGDCSMLTRETLFRRRVYAWRGRCSPCHLDDNEVDAPKWI
                     ATGACELGSLTTMRNVSGRGLVDLDDPAASLLLLKPLAESAGGVKHGGHDKFAGVDDP
                     AYVDFLAWISREAACTP"
     gene            2084..>2451
                     /locus_tag="VGB85_30090"
     CDS             2084..>2451
                     /locus_tag="VGB85_30090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138388.1"
                     /translation="MSPTPSPRASDRIWQRARERKVTAALGRLPDAPSELELLRVSCE
                     VAQAALRPLLESRRLAEAVLGEARAKPDPARSVRRRSLFGDTPERATEAVVVDLWNQL
                     ARHRARWVIVFDAVEQADEAT"
BASE COUNT          329 a          931 c          886 g          305 t
ORIGIN      
        1 ggcggacggc ggcgaggtgc gagccggcgt cagcctgccg ctatggcaag gccgcgcggt
       61 cgaccgccgg cgcaccgacc tgcgtgtcac ggcgctcgcg caggacgggg ccgcggcgga
      121 cctcgaggcc cgcatgatcg agctcgagcg cgcggcgggg cgcgcctact gggcctgggt
      181 cggcgccggc ctgcgcctgc aggtgcagcg cagcctgctg gccctcgccg aggaccgcga
      241 cgaggcgctg cgcaagcaga tcgccgcggg cagcgtgccg gcggtcgagg gcctcgacaa
      301 ccgccgggcg atcctcgatc gcggggcccg catcgtcgcc gccgagcggg cgctgcagca
      361 ggcggccttc gagctcgcca ggcacctgcg cgacgccaac ggaaacctcg agacgcccgc
      421 cccggggcgg ttacccgacg cgttccccga gccgccgccg cccgccgccg acgcgatcga
      481 gcagacgatc cgcgaggcat gggcgcgccg gcccgacctg ctgcgcctgc gcctgcagcg
      541 tgaggcggcc gcgatggagc tcaagctggc gcgcggtgat cgttcgccga agatcgacct
      601 cgacgcctac ggcgccaagg acatcggcgc gaccgcgccg gaatacgcgt acctgcggcc
      661 ggccgaattc gtcgccagcg tcagcatcga gctcccgctg gcgctgcgcg gcgcccgggg
      721 caagctacgg cgcgccgagg cgacgctcgc ccgcatcgac gccgagctgc ggatggcccg
      781 cgacgtgatc gcgctcgaga tccgcgacgc gcactcggcc ctggtcgccg cgcaccggcg
      841 ggtggccctc gcgcgcgagc agctcgcggc ggcccacgag ctcgagcagg cggagcgcag
      901 ccgctttcgc ctcggcgaca gcacgctctt gctggtcaac ctccgcgagc aagccgcggc
      961 cgacgcggcc cagcaggagg tcgacgcgct cgccgattat caccgcgcca gcgtcgacct
     1021 gcgcgccgcc gccggcctgt tcgggccatg agaggcgctc gcaggggtga tcatggcgtg
     1081 catgcggcct cgcgggagat ccacgccagg aagtcgacgt aggccgggtc gtcgacgccg
     1141 gcgaacttgt cgtggccgcc gtgcttcacc ccaccggcgc tctcggcgag cggcttgagc
     1201 agcagcaggc tcgcggccgg gtcgtcgagg tcgacgaggc cgcgccctga cacgttgcgc
     1261 atcgtcgtca acgagccgag ctcgcacgcg ccggtggcga tccacttcgg ggcgtcgacc
     1321 tcgttatcgt cgaggtgaca cggcgagcag cgcccgcgcc aggcgtacac gcgccggcga
     1381 aacagggtct cgcgcgtcag catcgagcag tcgccggggt cctcgtaggt gtccgtcttc
     1441 gggtcgtcgg cctcgcagct gtgctggacc ggggtgccgc acggcgcgtc gccgacgtaa
     1501 cacccgccgc atgtcgcggt cgcctcgatc cacgccagga acgccgcgta ctcctcgtcg
     1561 atcacccgtt gatcgatcaa cgggctcgcc ggatcggcgc gcgagatcca ccgcaagatc
     1621 gtgctcgacg ccggatcggc gaggtctacg aggccctttg tctcgaggca agccatcgtc
     1681 tgacacggcg tgtcctgcac gaacatctcg aggtcgatgc ccgacaggtg acaggtgttg
     1741 caggtcttgg gccggtcggt cgccagcaac ggcgcgatcc gccgggtgta gaggtcgtca
     1801 ctcagaccct cgcagtcgac cgcggtcgtc gtccccgtcg tcgcgcccgt cgacccgtcg
     1861 ccggtcgtcg cgtccacctc ggccgcggac ccctcgcgga cgcagcccgc cagggccagg
     1921 atcgccaaaa ccgccagtcg ctcgggacgc acgtcggcca gtacgacacc ggaacggcgg
     1981 cccgggaccg ctgcggcgga tcgtcgcacc ccctgcgtgc gcggccgcgc ccggcgtgct
     2041 gcgagccacc caacgggtcc cacgacccgg gtaccatcgg cccgtgagcc ccacgccctc
     2101 gccgcgagca tcggaccgga tctggcagcg cgcacgcgaa cgcaaggtca cggcggcgct
     2161 cggccgcctg ccggacgccc cgtccgagct cgagctgctg cgcgtcagct gcgaggtcgc
     2221 acaggccgcc ctgcggccgc tgctcgagtc acggcggctg gccgaggccg tgctcggcga
     2281 ggcgcgggcc aagcccgacc ccgcgaggtc ggtccgccgc cgctcgctgt ttggtgacac
     2341 ccccgagcgc gccaccgagg cggtcgtcgt cgacctgtgg aatcagctcg cccgccaccg
     2401 ggcccgctgg gtcatcgtgt tcgacgcggt cgagcaggcc gacgaggcga c
//
LOCUS       DASWNJ010001800         9058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_525862, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001800 DASWNJ010000000
VERSION     DASWNJ010001800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 9058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9058
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525862"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VGB85_30095"
     CDS             complement(<1..414)
                     /locus_tag="VGB85_30095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138389.1"
                     /translation="MSRKTVETVGEVLGRWVGKALAPVAAAAARGRKGRALHARGTVC
                     AAEVMAGAEDPELAPLAQRLAGAAIVRMSGALWRHATLPEMLGCAMRLRGPRTLTPEV
                     DPQDQDILFATARAPWAVPLAMLTTDRDDYLQNVYY"
     gene            complement(462..1127)
                     /locus_tag="VGB85_30100"
     CDS             complement(462..1127)
                     /locus_tag="VGB85_30100"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEY0138390.1"
                     /translation="MSKFVTRQCTRADCRLRFPVAADSTLGDRCPHCGTETAVDLGYD
                     SLDARVPAAGVAAGPAPTQVPGPAVSALLDNVRSLRNVGSMFRTADGAGLQHMHLGGF
                     TPTPAHPQMAKTSLGAESTVPWTLHRDGAAAAAMLRSAGHRLWALEGGPDAEDFYGVT
                     LSARTGPPIVLVLGHEVSGVDPRILACCEKVLRLPMLGSKGSLNVSVAFGIAVYGLRF
                     ARG"
     gene            1167..1532
                     /locus_tag="VGB85_30105"
     CDS             1167..1532
                     /locus_tag="VGB85_30105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138391.1"
                     /translation="MGYRDDRDAMVLQLAELERDNARLRDALDAAEGRAREVQEQRHE
                     QRRTAQKACVMCGGTLLPVAIFAGHDTRQPLPLSMSTLRFTSPDGGFTHAAPVHSLAC
                     SSCGFIHNFIDIRPTLPLR"
     gene            1891..3237
                     /locus_tag="VGB85_30110"
     CDS             1891..3237
                     /locus_tag="VGB85_30110"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0138392.1"
                     /translation="MIASLGLSASASAQPASPASGGAEVKPIRRFTPERNMVELGVFG
                     GVMIFNRSHDLYAPATTPGEPIRRPTADLGIRAAYFPLRFLGVEAELAALPAKYTAAA
                     SGPGGRGFIYGLRGHAILQLPLYRVVPFVLGGYGLMGVSSASTVAGKDMDPVGHLGGG
                     VKYYLNRWVALRADVRGYIGAEARQWPGTVGHFETLLGVSVTLNRGKPKADPDPDHDG
                     FRADDRCPTDPGVAPDGCPAQDSDGDGFKDPADKCPQEPGVAPDGCPAQDSDGDGFTD
                     DVDKCPDVAGVAPDGCPPPDADGDGIPDKDDKCVQEPETRNGYEDTDGCPDEVPKSVT
                     QYTGVIQGIYFDFNKDTIRKTSAPTLDAAAKIFQEFPDLRVEITGHADDLGSREYNIQ
                     LSERRAEAVKRYLVGKGVAAERIETRGVGPDAPIDNNKSETGRAKNRRIEFKILIK"
     gene            3333..4961
                     /locus_tag="VGB85_30115"
     CDS             3333..4961
                     /locus_tag="VGB85_30115"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0138393.1"
                     /translation="MSLLLLLPVVACKSDDLAAATTSGTETTVASSTTDPTTSGGMTD
                     PSTGTTIEETTVDPTTGPLPSTTDLSATDTSTTDDSTTDASSSTGDPPPMCTDGMLDP
                     GEACDDGNQVDGDECTNDCSLPVCGDGILHEGVEACDDGNQDDDDACLPTCEAATCGD
                     GVVQAGVEGCDDGNQVDDDACTNTCTLPACGDGLVQMGEQCDDANAVDTDDCLGVCTT
                     AACGDGFIHDGFETCDDAGDSPTCDEDCSTAQCGDGDVNAQAGETCDESGESATCDDD
                     CTDPQCGDGHVNPAFGEACDDSGESATCDDDCTPATCGDGNANAAAGEQCDTSGESAS
                     CDADCTTATCGDGQLNMLAGEQCDDGNTAEGDGCSSQCKPHKVVFASSLAVNGALGGL
                     DGADAQCQQLAVAAGLKGTYRAWLSDSTGSPSTRFTKSAIPYVRRDGVVIANNWADLT
                     DGTLAAAINLSEIKTTPGSDANICNGLKGLALTDTATDGTLFNAMGTCLDWTSAAANQ
                     VTGGGHFSSANSNWSAYFCIYTCNWKKALYCFQQ"
     gene            4973..6067
                     /locus_tag="VGB85_30120"
     CDS             4973..6067
                     /locus_tag="VGB85_30120"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEY0138394.1"
                     /translation="MPTITLDDLRRYAVARTLFKPTTLGRAILRLGFVQADPIRAPAR
                     AQDLTLRHRVQGYVAGELERRYPRLPIEEDFFVNYGFLPREHLTLMHPRVAKRAWDRA
                     TLRRAADVLAFVREHGRVHPREVAAQFSHGRVTNYWGGSSNATTHLLDGMHYRGMLRV
                     ARRDGGTRVYEAADHPQGDDGPDGRARRAAALIELVVRKYAPLPGASLTYLVRLLGHG
                     APQLTVQTQAALRLAREHLARATLDGVHWYWPADENPASRRYAPDEQVRLLAPFDPVV
                     WDRRRFELLWGWTYKFEAYTPAARRKFGYYALPLLWHDDVIGWANVSLQDGRLTPQFG
                     YASGKAPRSGAFRHALDDDLQRMTLLLGCS"
     gene            6419..8464
                     /locus_tag="VGB85_30125"
     CDS             6419..8464
                     /locus_tag="VGB85_30125"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0138395.1"
                     /translation="MRARSMFLGLAAALAACGDNSSDSGLSGSSSSTLPGTSDPTTTP
                     TSTSGQTGSATEADDGTASASDSATTSTTALPTTSQGSVTDTSDAGDGTGTTDDPDTG
                     VVDFCTPPPAGPAEDVPQNDACDIALQTGSFNPVIEWKYGSAPSFYGPPVVGQVIDTN
                     KSGGIDSQDLPHAFIYETGKVVALRGDGSGVAWATVKADYGYEGSLALGDLEGDGWPE
                     IITAKYSHVCALNGQTGAEKWCTDVPPGTSCPYSYNNPSIADMDGDGVAEVVLGSAVL
                     SATGTLLGAGALGHGGSALGQSDLSAVVDLDADGVLEVVTGNAAYDMHGNILWQNGEV
                     DGTVAVADFDLDGRGEIVKTHGGNIYGMESDGTHVWGPVSYGSLLGAAAIDDLDGDGT
                     PELVVAGQNNLVALEWGGDVIWTAPVSDSSGAAGPVLFDFEKDGYPEVLYADEVAIRF
                     FSGLDGTLKFQSMEHSSATAFETPVVVDIDGDNHVEIVLGHGYGNAQIGSVTIYGDAD
                     NSWPPGRKIWNQHAYHITNVSDLGAIPSQYQPNWIPGFNSFRSGDAGQLPGEYHDLQV
                     EILGVCETQCEMGTFFMTARPRNAGTLEAPAGLPVTVRAGVGGDIVVTLETTQPIPAG
                     KTGELLFFEFAAKDLAQSQPVITVDDTGVGEGKLFECDELNNTAVWPQAVCPTVEPG"
     gene            complement(8619..>9058)
                     /locus_tag="VGB85_30130"
     CDS             complement(8619..>9058)
                     /locus_tag="VGB85_30130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138396.1"
                     /translation="AEPVVSPHTGVTATNAPQPPVDVPARQGVETPPLPPQPPLMPSA
                     AAVAESEAFAAAMPAVRQQVQAAIARQKPAIRKACWTGEVPESASFPVEAGFDAEGNL
                     LTLSIGDDRAAPGISPCVREQTLQLDIDAPGVPITVQATLDLP"
BASE COUNT         1468 a         3466 c         2932 g         1192 t
ORIGIN      
        1 gtaatacaca ttttgcaggt agtcgtcgcg gtcggtcgtc agcatcgcca gcggaaccgc
       61 ccacggcgcc ctcgcggtcg cgaacaaaat gtcctggtcc tgcgggtcga cctccggggt
      121 cagcgtgcgc ggaccgcgaa ggcgcatcgc gcagccgagc atctccggca aggtggcgtg
      181 ccgccacagc gcccccgaca tgcgcacgat cgccgcgccc gcgaggcgct gcgccagtgg
      241 ggccagctcc gggtcctccg cgcccgccat cacctcggcc gcgcacaccg ttccgcgcgc
      301 atgcagggcc cggcccttgc gccccctggc cgccgccgcc gccacggggg cgagcgcctt
      361 cccgacccac cgcccgagca cctcgccaac cgtctcgacc gtcttgcgcg acatggccag
      421 tccttgggcc gggacggccc gccacgcaac cacaacaaag ttcacccccg cgcgaagcgc
      481 agcccgtaca ccgcgatccc gaacgccacg ctgacgttga gcgacccctt gctgccgagc
      541 atcggtaatc tcaacacttt ctcgcagcac gcgaggatcc gcggatcgac ccccgagacc
      601 tcgtgcccca gtaccagcac gatcggcgga cctgtccttg cagacagagt cacaccataa
      661 aaatcctcgg cgtcgggtcc gccctcgagc gcccacaggc ggtggcccgc gctgcgcagc
      721 atcgccgccg ccgccgcgcc gtcgcggtgc agcgtccatg gcaccgtcga ctccgcgccg
      781 agcgaggtct tggccatctg cgggtgcgcc ggcgtcggcg tgaagccgcc gaggtgcatg
      841 tgctgcagcc ccgccccgtc ggccgtgcgg aacatcgaac cgacgttgcg caggctgcgg
      901 acgttgtcga gcagcgccga gaccgcgggc cccgggacct gcgtcggcgc cggaccggcc
      961 gccacgcccg ccgccggcac gcgcgcgtcg agcgagtcgt agccgaggtc gaccgccgtc
     1021 tccgtcccgc agtgggggca gcggtcgccg agcgtggagt cggccgcgac cgggaagcgc
     1081 aggcggcagt cggcgcgggt gcattgccgg gtgacgaact tggacacggt gccgcgaggg
     1141 ttagcacttc ctgctatcgt cggggcatgg gttatcgcga cgaccgcgac gccatggtcc
     1201 tgcagctcgc cgagctcgag cgggacaacg cgcgcctgcg cgacgccctc gacgccgccg
     1261 agggccgcgc gcgcgaggtc caggagcagc gccacgagca gcgccgcacc gcgcagaagg
     1321 cctgcgtcat gtgcggcggc accctgctcc cggtcgcgat cttcgccggt cacgacactc
     1381 gccagccctt gccgctgagc atgagcacgc tgcgcttcac ctcgcccgac ggcggcttca
     1441 cccacgccgc ccccgtgcac tccctcgcct gctcgagctg cgggtttatc cacaacttca
     1501 tcgacatccg cccgaccttg cccctgcgct gacctccgcc acccgggtcc gcgaacgctg
     1561 aacatccgca ccgggatcac cccgaccccc ggcgctcagt ttcgccacca ggacggtgaa
     1621 cggcgagcct gccgaagtgt gaaggaccct cccacgcgag ctcgacctca tggggcgaac
     1681 atgacggccg gcgtgaaagc tcgtcgaagc ggcgcgcccg ggatgaagaa aagttcacat
     1741 ctgcttcacg cgcccggcgg cgcgggctcc ccctcgacga cgccgatgga gatggcgcct
     1801 gccctgtcgg ccaactgccg ctcgctcacc cccacgccga tctgatagac caagagcagt
     1861 catatgcgct cgactccgct ggtcttgatc atgatcgcgt ccctgggact gtcggcctcc
     1921 gcgtccgccc agcccgcgag cccggcgagc ggcggcgcgg aggtcaagcc gatccggcgc
     1981 ttcacgccgg agcgcaacat ggtcgagctc ggcgtcttcg gcggcgtgat gatctttaat
     2041 aggtcgcacg acctgtatgc cccggcgacc acgccgggcg agccgatccg gcggccgacc
     2101 gcggacctcg ggatccgcgc ggcatatttc cccctgcgct tcctcggggt cgaggccgag
     2161 ctcgccgcgc ttccggccaa gtacacggct gcggcgagcg gacccggcgg tcgcggcttc
     2221 atctacggcc tgcgcggcca cgcgatcctg caattgccgc tgtaccgcgt ggtgcccttc
     2281 gtgctcggcg gctacgggct gatgggcgtg tcgtcggcgt cgacggtcgc cggcaaggac
     2341 atggacccgg tcggccacct cggcggcggc gtcaagtact acctcaaccg ctgggtcgcg
     2401 ctacgggccg acgtgcgcgg gtacatcggc gccgaggcca ggcagtggcc cggcaccgtc
     2461 gggcacttcg agaccctgct cggcgtcagc gtcacgctca accgcggcaa gccgaaggcc
     2521 gacccggacc cggaccacga cggcttccgg gccgacgacc gctgccccac cgaccccggc
     2581 gtcgcccccg acggctgccc cgcgcaggac agcgacggcg acggcttcaa ggacccggcc
     2641 gacaagtgcc cgcaggagcc tggcgtcgcc cccgacggct gccccgcgca ggacagcgac
     2701 ggcgacggct tcaccgatga cgtcgacaag tgccccgacg tcgccggcgt cgcgcccgac
     2761 ggctgcccgc cgcccgacgc cgacggcgac gggatccccg acaaggacga caagtgcgtc
     2821 caggagccgg agacgcgcaa cggctacgag gacaccgacg gctgccccga cgaggtgccc
     2881 aagtcggtga cgcagtacac gggcgtgatc cagggcatct acttcgattt taataaagac
     2941 acgatccgca agacctcggc gccgacgctc gacgccgcgg cgaagatctt ccaggagttc
     3001 ccggacctgc gggtcgagat caccgggcac gccgacgacc tcggatcgcg cgaatacaac
     3061 atccagctgt cggagcgccg cgcggaggcg gtcaagcgct acctcgtcgg caagggggtc
     3121 gcggccgagc ggatcgagac gcgcggcgtc ggacccgacg caccgatcga caacaacaag
     3181 tcggagaccg gccgcgccaa gaaccgccgc atcgagttca agatcctgat caagtgactc
     3241 gatttcgcgc ccacgtcgtc gtccgcgcgg cgtgggccct ctggagatag atgaacatga
     3301 cttatccgct cccatggcca tcgctgcgca gcatgtcgct gctcctgctc ctccccgtcg
     3361 tcgcatgcaa gagcgacgac ctcgccgccg cgacgacctc cggcacggag accacggtcg
     3421 cgtcgtcgac caccgacccg acgacgagcg gaggcatgac cgacccgagc accggcacca
     3481 cgatcgagga gaccacggtc gacccgacga ccggcccgct gccctcgacg accgatctct
     3541 cggcgaccga cacgtcgacg accgacgact cgacgaccga cgcgagctcg agcaccggcg
     3601 acccgccgcc gatgtgcacc gacggcatgc tcgaccccgg cgaggcctgc gacgacggca
     3661 accaggtcga cggcgacgag tgcaccaacg actgctcgct gccggtctgc ggcgacggca
     3721 tcctccacga gggcgtcgag gcgtgtgacg acggcaacca ggacgacgac gacgcgtgcc
     3781 tgccgacctg cgaagccgcg acctgcggtg acggcgtcgt ccaggccggc gtcgagggct
     3841 gcgacgacgg caaccaggtc gacgacgacg cgtgcaccaa cacctgcacg ctcccggcct
     3901 gcggcgacgg cctggtccag atgggcgagc agtgcgacga cgccaacgcc gtcgacaccg
     3961 atgactgcct cggcgtgtgc accacggcgg cctgcggcga cggcttcatc cacgacggct
     4021 tcgagacctg cgacgacgcc ggcgactcgc cgacctgcga cgaagattgc tcgaccgcgc
     4081 aatgcggcga cggcgacgtc aacgcccagg ccggcgagac ctgcgacgag tcgggcgagt
     4141 ccgccacctg cgacgacgac tgcaccgacc cgcagtgcgg cgacggtcac gtcaacccgg
     4201 cgttcggcga ggcctgcgac gacagcggcg agtcggcgac ctgcgacgac gactgcacgc
     4261 cggcgacctg cggcgacggc aacgccaacg ccgcggccgg cgagcagtgt gacacgtccg
     4321 gcgagtcggc gagctgcgac gccgactgca cgaccgcgac ctgcggcgac ggccagctca
     4381 acatgctcgc cggcgagcag tgcgacgacg gcaacaccgc cgagggcgac ggctgctcgt
     4441 cgcaatgcaa gccgcacaag gtcgtgttcg cctcgagcct cgccgtcaac ggcgccctcg
     4501 gcggcctcga cggcgcggac gcacaatgtc agcagttggc ggtcgcggcc gggctcaagg
     4561 gcacctaccg cgcgtggctc agcgacagca ccggctcgcc ctcgacgcgc ttcaccaagt
     4621 cggcgatccc ctacgtgcgg cgcgacggcg tcgtgatcgc caacaactgg gccgacctca
     4681 ccgacggcac cctcgccgcc gcgatcaacc tctccgagat caagaccacc ccgggcagcg
     4741 acgccaacat ctgcaacggc ctcaagggcc tcgccctcac cgacaccgcc accgacggca
     4801 ccctgttcaa cgccatgggc acctgcctcg actggaccag cgccgccgcc aaccaggtga
     4861 ccggcggagg ccacttctcg tccgccaaca gcaactggtc ggcctacttc tgcatctata
     4921 cgtgcaactg gaagaaggcc ctgtactgtt ttcaacagtg aggcccgggg ccatgcccac
     4981 gatcacgctg gacgacctgc ggcgctatgc ggtcgcccgc acgctcttca agccaaccac
     5041 gctgggccgc gccatcttgc ggctgggttt cgtgcaagcg gacccgatcc gcgcgccggc
     5101 gcgggcgcag gacctcacgt tgcgccaccg tgtgcagggg tacgtcgccg gcgagcttga
     5161 gcggcgctac ccgcgattgc ccatcgaaga ggacttcttc gtcaactacg gcttcctgcc
     5221 gcgtgagcat ctgacgctca tgcacccacg cgtggccaag cgcgcgtggg accgcgccac
     5281 attacgccgc gcagccgatg tgctcgcctt cgtgcgcgag catggccgcg tgcacccgcg
     5341 cgaggtggca gcgcaatttt cgcatggtcg agtcaccaac tactggggcg gctcgtcgaa
     5401 cgccaccacg cacttgctcg acggcatgca ctaccgcggc atgctgcgtg tggcgcggcg
     5461 agacggcggc acgcgtgtgt acgaagccgc cgaccatccg caaggcgatg acggccccga
     5521 cgggcgcgca cgccgggctg cggccctcat cgagctcgtc gtacgcaagt atgcaccgct
     5581 gcctggcgcg agcctcacgt acctcgtgcg cctgctcggc cacggcgcgc cccagctcac
     5641 ggtgcagacg caagccgcgc tgcggctggc gcgtgaacat ctggcccgcg ccaccctcga
     5701 cggcgtgcac tggtattggc cggccgacga aaaccccgcc tcgcgtcggt atgcacccga
     5761 cgaacaggtg cgcttgttgg cccccttcga ccccgtcgtc tgggaccggc gccgcttcga
     5821 gctgctatgg ggctggacgt acaagttcga ggcctacacg ccggccgcac gacgcaagtt
     5881 cggttactac gcactaccgt tgctgtggca tgacgacgtc atcggctggg ccaacgtctc
     5941 gctgcaagac ggacggttga cgccccaatt cgggtacgcc agcggcaagg cgcccaggag
     6001 cggagcgttc aggcacgcgc tcgacgacga cctgcaacgc atgaccctgc tcctgggctg
     6061 ctcgtagccg cgtcatggcc cgcacattct acgggggtga catcgggacg ccgagggtca
     6121 ggatcgcggc atggcagtgg acgccgcggc cgggctcaag ggcacctacc gcgcgtggct
     6181 cagcgacagc accgctcccc ggcgtcgccg ggcgagcacc aggagcaggt agagtgcacg
     6241 caggcgcgca gcgccgggac caccagggat cgttcgacct cgacgcgccc cgacgtcacc
     6301 ggcctcgcga tatcccgcac gagcattccc ggccagcgcg agcgcgcatc gtctctgcct
     6361 cgccagcgcc gtatgtccgc ggttgacagc ccggcgagcg tccgggacag tgccagtcat
     6421 gcgtgcaaga tcgatgttcc tcggcctcgc ggccgctctg gcagcctgcg gggacaacag
     6481 ctccgactcc ggcctgtccg gatcgtcctc gtccaccctc cccgggacga gcgaccccac
     6541 gacgacgccg acgtcgacat ccggccagac gggctcggcc accgaggcgg acgacggcac
     6601 agcctccgcc tccgactccg ccaccacctc gacgaccgcg ctccccacca ccagccaggg
     6661 ctccgtcacc gacaccagtg acgccggcga cggcaccggc accaccgacg accccgacac
     6721 cggcgtcgtc gacttctgca cgccgccgcc cgccggcccc gccgaggacg tgccgcagaa
     6781 cgatgcctgc gacatcgcgc tgcagacagg cagcttcaac cccgtcatcg agtggaagta
     6841 tggctcggcg ccttcgttct acggaccgcc ggtcgtgggt caggtcatcg acaccaacaa
     6901 atcgggcggc atcgactcgc aggacctccc ccacgccttc atctacgaga ccggcaaggt
     6961 cgtcgccctg cgcggcgacg gcagcggcgt cgcctgggcc accgtcaagg ccgattacgg
     7021 ctacgagggg agcctcgcgc tcggcgacct cgagggcgac ggctggccgg agatcatcac
     7081 cgcgaaatac agccacgtgt gcgcgctcaa cggccaaacc ggcgccgaga agtggtgcac
     7141 cgacgtcccg cccggcacca gctgtccgta cagctacaac aacccttcga tcgccgacat
     7201 ggacggcgac ggcgtcgccg aggtcgtgct cggcagcgcc gtcctcagcg ccaccggcac
     7261 gctcctcggc gccggcgccc tcggccacgg cggctcggcg ttggggcagt ccgacctctc
     7321 ggcggtcgtc gacctcgacg ccgacggcgt gctcgaggtc gtcaccggca acgccgccta
     7381 cgatatgcac ggcaacatct tgtggcaaaa cggtgaggtg gacggcaccg tcgccgtcgc
     7441 cgacttcgac ctcgacggcc gcggcgagat cgtcaagacc cacggcggca acatctacgg
     7501 catggagtcc gacggcaccc acgtgtgggg ccccgtcagc tacggcagcc tcctcggcgc
     7561 cgccgccatc gacgacctcg acggtgacgg cacccccgag ctcgtcgtcg ccggccagaa
     7621 caacctcgtc gccctcgagt ggggcggcga tgtcatatgg accgcgcccg tcagcgacag
     7681 cagcggcgcc gccggtcccg tcctgttcga cttcgagaag gacggctacc ccgaggtcct
     7741 ctacgccgac gaggtcgcca tccgcttctt ctcgggcctc gacggcaccc tcaagttcca
     7801 gtcgatggag cactcgagcg cgaccgcctt cgagacccct gtcgtcgtcg acatcgacgg
     7861 cgacaaccac gtcgagatcg tgctcggcca cggctacggc aacgcccaga tcggatcggt
     7921 gacgatctac ggcgacgccg acaactcctg gccacccggt cgcaagatct ggaaccagca
     7981 cgcctaccac atcacaaacg tctctgacct cggcgccatc cccagtcagt accagcccaa
     8041 ctggatcccc ggcttcaaca gcttccgctc cggcgacgcc ggccagctgc ccggcgagta
     8101 ccacgacctc caggtcgaga tcctcggcgt ctgcgagacc cagtgcgaga tgggcacctt
     8161 cttcatgacg gctcgcccgc gcaacgccgg caccctcgag gcccccgccg gcctccccgt
     8221 caccgtccgt gccggtgtcg gcggcgacat cgtcgtcacg ctcgagacga cccagccgat
     8281 ccccgccggc aagaccggcg agctcctctt cttcgaattc gccgccaagg acctcgccca
     8341 gtcccagccc gtcatcaccg tcgacgacac cggcgtcggc gagggcaagc tgttcgagtg
     8401 cgacgagctc aacaacaccg ccgtctggcc gcaggccgtc tgccccaccg tcgaacccgg
     8461 ctgacccgcc ccaccccgtg ctccacgcgg gcggcatcgg ccccgagcag gtcgatgccc
     8521 gggccaccag gtgcctccgc ccacaacacg atcgtcgtcg aggcggcgcg cgagctcgcc
     8581 gggacgcgga gtaggacgag gtcctcggac acgtcgtttc agggcaggtc gagcgtcgct
     8641 tgcaccgtga tcggcacgcc gggcgcgtcg atgtcgagct gaagtgtttg ctcgcgcaca
     8701 cacggcgaga tgccgggcgc cgcccggtcg tcgccgatcg acagcgtgag caggttgccc
     8761 tctgcgtcga agccggcctc gaccgggaac gacgccgact ccggcacctc gccggtccag
     8821 cacgccttgc ggatcgccgg cttttgccgg gcgatcgccg cctggacctg ctgcctgacc
     8881 gcgggcatcg ccgcggcgaa cgcctcgctc tcggcgactg ccgccgcgct gggcatcagc
     8941 ggcggctgcg gtgggagcgg cggcgtctcg actccctgcc gggcgggcac atccaccggg
     9001 ggctgcggag cgttcgtcgc cgtcacgccc gtgtgcggcg acaccaccgg ctcggcgg
//
LOCUS       DASWNJ010001801         6187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_383954, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001801 DASWNJ010000000
VERSION     DASWNJ010001801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383954"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1759)
                     /locus_tag="VGB85_30135"
     CDS             complement(<1..1759)
                     /locus_tag="VGB85_30135"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0138397.1"
                     /translation="MAFNREKALTAAAKFVSKGQHDRAAKEYLAIIEADPNDTRSWLL
                     LAEALTNAGDKPGAIERYLHVAEGYATANDPQKAIAVYRKVLGLDPNRLDIQTRIAGL
                     YKDMGRTADAVGAYEFVAQAYFQAGQIAEGLEGFRMVAELEPAAVGKRLRLAELYSRE
                     GMVAQAVEHFRLAADRLLTDRRTDDYIRVAERLIYHKEDDLPVLRSLAQIYLKQGENR
                     RALVKLNALLRAAPADAEGLELLGETFVAIGKVDKAISVVMELAREQRKGGRKNKETA
                     ARVLRKALGWNPENADEIRKIATEIEAEVAALPPDPEPGDKPREEGMDLDVDVVEEEA
                     AGSSTDLDIDVDIDEDEEVRKVEEVPSRPFAPVGANVAPSAVEDAEEGVGDVEKILLE
                     ARVYVKYKMFEHALGHLDAVFVRDANHVAALELQAQILVELGRNADAADSFVRLAQLV
                     SSTDPRQARTHLAEAQKLVPDHTKADMVLAALDSGDSGGGGETAPVLSLKDDPASAPA
                     TVDAPEPAEASAPGETAPAGDANADKADEEPADQVAADATPDTRTADSEPATSPDGTD
                     TKKSDSDAPQSDDVAQPRGD"
     gene            complement(1848..3230)
                     /gene="accC"
                     /locus_tag="VGB85_30140"
     CDS             complement(1848..3230)
                     /gene="accC"
                     /locus_tag="VGB85_30140"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEY0138398.1"
                     /translation="MITEIRKVLIANRGEIALRVIRACRELGLKTVAVYSTADEHALH
                     VRFADEAVCIGPGQAAQSYLNIKHIIAAAELTGADAVHPGYGFLAERAEFAEIVQKCG
                     MTFIGPDPVHMQAMGDKVRARATMEKSGVPVLPGTGVLTSAAEAIEAAERIGLPVILK
                     ASAGGGGRGMKIIRDMRALPSTLETAQLEAFKAFGNGDMYLERYVTSPRHVEVQIVAD
                     KFGDAIHLLERECSVQRRHQKVLEEAPCAVLDDNLRAAMCAAAVKAVKAINYHSLGTL
                     EFLLDGDRFYFMEMNTRVQVEHCVTEMITGVDLVQEQIRLATGEPLAHLDREHKPRGH
                     SIECRINAEDPVTFAPRPGTISALHFPGGFGVRVDSHCYTGYTVSPHYDSMVAKLIVH
                     APDRPRAISRMRRALGECVIEGLTTNLPLHRWLLKQEAFVSGKYDTHFLEANLDPEAI
                     RSEADADAQA"
     gene            complement(3227..3730)
                     /gene="accB"
                     /locus_tag="VGB85_30145"
     CDS             complement(3227..3730)
                     /gene="accB"
                     /locus_tag="VGB85_30145"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEY0138399.1"
                     /translation="MSSDPRDTNGLPDLRYIRELAKVFRQYDLDEIEIENGEQRVLLR
                     RGDASLGGAPGQAGFAPRTPRHEPEPVAPAPAPAAPAAPVAGSGDSFITSPFVGTFYR
                     SSGPDTPAFVDVGSTVQRGQTLCIVEAMKLFNELEAEFPCVIEAVLLDNAQPVEYGAK
                     LFRVRKL"
     gene            complement(3786..4283)
                     /gene="aroQ"
                     /locus_tag="VGB85_30150"
     CDS             complement(3786..4283)
                     /gene="aroQ"
                     /locus_tag="VGB85_30150"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEY0138400.1"
                     /translation="MSRPVSPTGSPRVLVLHGPNLNLLGTREPEIYGRGTLAELDGSL
                     HALARELGVNLTTQQSNSEGALIDAIQGCRAGGSHPQDGLVINPGGYTHTSVAIHDAL
                     RSVSIPAIEVHLSNLYARESLRHTSVTGVACAGVIMGLGTASYLLALRHLANVLAAVP
                     ASPAG"
     gene            complement(4332..4673)
                     /locus_tag="VGB85_30155"
     CDS             complement(4332..4673)
                     /locus_tag="VGB85_30155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138401.1"
                     /translation="MLDTLRSIVEKIPGAKAAVLMGLDGYAVEKYVGDGSLGDIEQVS
                     TEFIEMVKVARALDLGNVSDITVRAENGTLLARFLSEGYFAVVLMSETGNFGKGRWVL
                     RNAANELLASL"
     gene            complement(4894..6048)
                     /gene="aroB"
                     /locus_tag="VGB85_30160"
     CDS             complement(4894..6048)
                     /gene="aroB"
                     /locus_tag="VGB85_30160"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEY0138402.1"
                     /translation="MTETKTKQRPRPLVLDVHVPAENHSYPVLIGRGAIGRLGSELQQ
                     RLGRAGTVALVSDTTVAPLHGAAVQANLEAQGFTVVPIVIPAGEAHKTTGTLLEVLGA
                     MIVSGMGRRDAVVALGGGVIGDLSGLAAALFMRGVPVIQCPTTLLAQVDASVGGKVAV
                     DVPAGKNMVGAFHFPLFVLIDPEVLSTLRDRELAQGLAEMLKHALLFAPDHLQALLDN
                     AKAIYARDFDVLSPLIATSVGLKAACVGRDPWETGEAGKGRVLLNLGHTVGHAIESAS
                     NYSVPHGEAVALGLRAAARVSEARGIAEPGLEATVVDALTRLRLPTGLDEWLEGDRGA
                     AVERALVVDKKRSAGTVSYVALARVGEPSVVSLSLRDLLNLLRQRSAPAE"
BASE COUNT          886 a         2051 c         2239 g         1011 t
ORIGIN      
        1 tgtcgccacg cggctgcgcg acgtcgtccg actgcggcgc gtcgctgtcc gacttcttcg
       61 tgtcggtccc gtccggcgag gtcgccggct cactgtcggc agtgcgcgtg tcgggggtcg
      121 catccgcggc gacctggtcg gccggctcct cgtcggcctt gtcggcgttc gcgtcgccag
      181 ccggagccgt ctcgcccggg gcgctcgcct ccgcgggctc gggtgcgtcg acggtcgcgg
      241 gtgcgctggc cgggtcgtcc ttgagcgaga ggacgggggc tgtctcgccg ccgccaccgc
      301 tgtcgccgct gtcgagggcg gcgaggacca tgtcggcctt cgtgtggtcg gggaccagct
      361 tttgcgcctc ggcgaggtgg gtacgggcct ggcgcgggtc ggtgctgctg accagctgcg
      421 cgaggcggac gaagctgtcg gcggcgtcgg cgttgcgtcc gagctcgacg aggatctgcg
      481 cttgcagctc gagggcggcg acgtggttgg cgtcgcggac gaacacggcg tcgaggtgcc
      541 cgagcgcgtg ctcgaacatc ttgtatttca catagaccct ggcctcgagc aggatcttct
      601 cgacgtcgcc gacgccctcc tcggcgtcct caaccgcgct gggcgccacg ttggcgccca
      661 cgggagcgaa agggcgggag ggtacctcct cgaccttgcg tacctcctcg tcctcgtcga
      721 tatcgacgtc gatgtcgagg tcggtgctgc tgccggcggc ctcctcctcg acgacatcga
      781 cgtcgagatc catcccctcc tcacgcggct tgtcgccggg ctcgggatcg ggtgggagcg
      841 cggcgacttc ggcctcgatc tccgtggcga tcttgcggat ctcgtcggcg ttctcggggt
      901 tccagccgag cgccttgcgc agcacccggg cggcggtctc cttgttcttg cggccgccct
      961 tgcgctgctc gcgggccagc tccatcacga cgctgatcgc cttgtcgacc ttgccgatcg
     1021 cgacaaaggt ctcaccgagc agctcgagac cctcggcgtc ggccggcgcg gcgcgcagca
     1081 gggcgttgag cttgaccagc gcgcggcggt tctcaccttg cttgaggtag atctgcgcca
     1141 gcgagcggag gaccgggagg tcatcctctt tgtggtaaat gaggcgctcg gcgacgcgga
     1201 tgtagtcgtc cgtgcggcgg tcggtcagca ggcggtcggc ggcgaggcgg aagtgctcga
     1261 cggcctgggc caccatgccc tcgcgtgagt acagctcggc gaggcgcagg cgcttgccga
     1321 cggcggccgg ctcgagctcg gcgaccatgc gaaagccctc gaggccctcg gcgatctggc
     1381 cggcctggaa gtaggcctgg gcgacgaact cgtacgcgcc gacggcgtcg gcggtgcggc
     1441 ccatgtcctt gtacagcccg gcgatgcggg tctggatgtc gaggcggttg gggtcgaggc
     1501 cgaggacctt gcggtagacc gcgatcgcct tctgcgggtc gttggcggtc gcgtagccct
     1561 ccgcgacgtg caggtaacgc tcgatcgcgc cgggcttgtc accggcgttg gtcaacgcct
     1621 cggcgagcag caaccacgaa cgggtgtcgt tcgggtccgc ctcgatgatc gcgaggtact
     1681 ccttggcagc acgatcgtgc tggcccttgg agacgaattt cgcggccgcc gtcagggcct
     1741 tttcccggtt gaacgccacc cccgctgctc actcctctac gccgaccggc gccgcatcgt
     1801 catcgttcaa gagtccgaag aagtggttcg agagtccgaa gaagtggtta tgcctgtgcg
     1861 tctgcatcgg cctcggacct gatcgcctcg gggtccaggt tggcctcgag gaagtgggta
     1921 tcgtatttac cactgacgaa ggcctcttgc ttcagcaacc agcggtgaag gggcagattc
     1981 gtcgtgagcc cctcgatcac gcattcgccc agcgcccggc gcatccgtga gatggcccgc
     2041 ggtcggtccg gggcgtggac gatgagcttg gcgaccatgg agtcgtagtg gggcgaaacc
     2101 gtgtaccccg tgtagcagtg gctgtcgacc cggacgccga agccgcccgg gaagtgcagg
     2161 gcggagatcg tccctggccg cggcgcgaag gttacggggt cctcggcgtt gatccggcac
     2221 tcgatggagt gtccccgcgg cttgtgctcc cggtcgaggt gcgcaagcgg ctcacccgtc
     2281 gccaggcgga tctgctcctg gacgaggtcg acgccggtga tcatctccgt cacgcagtgc
     2341 tcgacctgca cccgcgtgtt catctccatg aagtagaagc ggtcgccgtc gaggagaaat
     2401 tcaagggtcc cgagcgagtg atagttgatc gccttgaccg ccttgaccgc ggcggcgcac
     2461 atcgcggcgc gcaggttgtc gtcgagcacc gcacacgggg cctcttcgag gaccttctgg
     2521 tggcgccgct ggaccgagca ctcgcgctcg agcaggtgga tcgcgtcgcc gaacttgtcg
     2581 gcgacgatct ggacctcgac gtggcgcggg ctggtcacgt agcgctcgag gtacatgtcg
     2641 ccgttgccga aggccttgaa ggcctcgagc tgggcggtct ccagggtgct cggcagggcc
     2701 cgcatgtcgc ggatgatctt catgccgcgg ccgccgccgc cggccgaggc cttgaggatc
     2761 accggcaagc cgatgcgctc ggcggcctcg atggcctcgg ccgcgctcgt cagcacgccg
     2821 gtgccgggca gcacgggaac gcccgacttc tccatcgtcg cccgggcgcg gaccttgtcg
     2881 cccatcgcct gcatgtgcac ggggtcgggg ccgatgaagg tcatcccgca cttttggacg
     2941 atctccgcga actcggcgcg ctccgccagg aagccgtagc cggggtggac cgcgtcggcg
     3001 ccggtcagct cggcggcggc gatgatgtgc ttgatgttga ggtagctctg cgcggcctgc
     3061 ccggggccga tgcagacggc ctcgtcggcg aagcggacgt gcagggcgtg ctcgtccgcg
     3121 gtggagtaga cggcgaccgt cttgaggccc agctcgcgac atgcgcggat caccctcagg
     3181 gcgatctcgc cccggttggc gatcaggacc ttgcggatct ccgtgatcac agcttgcgta
     3241 cccggaacag cttggccccg tactcgacgg gctgggcgtt gtcgaggagc acggcctcga
     3301 tcacgcacgg gaactcggcc tcgagctcgt tgaacagctt catcgcctcg acgatgcaca
     3361 gggtctggcc gcgctgcacg gtgctgccga cgtcgacgaa ggccggcgtg tcggggcccg
     3421 acgagcgata aaaagtgccg acgaagggcg aggtgatgaa gctgtcgccg gagccggcga
     3481 ccggggccgc cggggctgcg ggcgccgggg cgggcgcgac gggctcgggc tcatgacgcg
     3541 gggtccgcgg cgcgaagccg gcctgaccgg gggcgccgcc gagggaggcg tcaccgcggc
     3601 gcagcagcac gcgctgctca ccgttctcga tctcgatctc gtcgaggtcg tactggcgaa
     3661 agaccttggc gagctcgcgg atgtaccgca gatcgggcag gccattggtg tcccggggat
     3721 cggacgacat gagactccat gaacaagaga gaacgagaga gaacgagagg acgtggacga
     3781 gagcgtcagc cggcgggcga ggccggaacc gcggccagaa cgttggccag atggcgtagc
     3841 gcgagcaggt aggaggcggt gccgaggccc atgatgaccc cggcacatgc tacgccagtc
     3901 accgaggtgt ggcgaagaga ttcgcgcgcg tagaggttgc tcaggtggac ctcgatcgcc
     3961 gggatgctca cgctgcgcag ggcgtcgtgg atcgctacgc tggtgtgggt ataaccaccg
     4021 gggttgatga cgaggccgtc ctgcgggtgg gagccgccgg cgcggcagcc ctggatggcg
     4081 tcgatgaggg cgccctcgct gttggactgc tgcgtcgtca ggttcacgcc gagctcacgg
     4141 gccaatgcat gcagcgagcc gtcgagctcg gcgagagttc ctcgtccgta gatctccggc
     4201 tcgcgggtgc cgagcaaatt gaggttcggc ccgtggagca cgagcaccct cggcgagccc
     4261 gtggggctca ctggtcggct catcgcctcg ctcatcgcct gctcactgcg cctgcgaagc
     4321 aggtcggtcg atcacaggct ggcgagcagc tcgttggccg cgttgcgcag gacccagcgg
     4381 cccttgccga agttgccggt ctcggacatc agcacgacgg cgaagtagcc ctcggacagg
     4441 aaacgagcca gcagcgtgcc gttctcggcg cgcacggtga tgtcggagac gttgcccagg
     4501 tcgagcgcgc gcgccacctt caccatctcg atgaactcgg tggagacctg ctcgatgtcg
     4561 ccgagactgc cgtcgcccac gtacttctcc acggcgtagc cgtcgaggcc catgagcaca
     4621 gcggccttgg cgcctgggat cttttcgacg atggatctga gggtatcgag catggtctct
     4681 cacaggccac aaacgggccg ggaaatcggt ctcaggcccg aatgggccct catgaaggca
     4741 gccgagcgcc gccttcgtgc cggtgcaggg tcgcagacac ggaccctcct ggcaagcagc
     4801 tttggtaggg caattgcgcc cgttgcgccg acccagggat cccccgcagg cgccatcagg
     4861 accgctcagg accccgttcg gtcgcggtgt cgctcactcg gccggcgcgg accgctggcg
     4921 gagtaagttg agcaggtcgc gcagggacag cgacacgacc gagggctcgc cgacccgcgc
     4981 cagcgcgacg tagctgacgg tgccggccga gcgcttcttg tcgacgacga gcgcccgctc
     5041 gaccgccgcg ccgcggtctc cctcgagcca ctcgtcgagg ccggtcggca agcgcaggcg
     5101 cgtcagggcg tcgaccacgg tcgcctcgag tccgggctcg gcgatgccac gcgcctcgga
     5161 cacgcgggcc gcggcgcgca gcccgagggc gacggcctcg ccgtgcggca cgctgtagtt
     5221 ggaggccgac tcgatcgcgt ggccgacggt gtggccgagg ttgagcagca cgcggccctt
     5281 gccggcctcg ccggtctccc acgggtcacg cccgacgcag gcggccttga gcccgaccga
     5341 ggtggcgatc aacggcgaga gcacgtcgaa gtcccgggcg tagatcgcct tggcgttgtc
     5401 gagcagcgcc tgcaggtggt ccggcgcgaa cagcagggcg tgcttgagca tctcggcgag
     5461 gccctgcgcg agctcgcggt cgcggagcgt gctcagcacc tcggggtcga tcagcacgaa
     5521 cagcgggaag tgaaacgccc cgaccatgtt cttgccggcg ggcacgtcga ccgcgacctt
     5581 gccgccgacc gacgcgtcga cctgcgcgag cagggtcgtc ggacactgga tcacgggcac
     5641 gccgcgcatg aacagcgccg cggcgagtcc ggagagatct ccgatcacgc cgccgccgag
     5701 agcgacgacc gcgtcgcgcc gacccatgcc gctgacgatc atcgcgccga gcacctcgag
     5761 cagggtgccc gtggtcttgt gggcctcgcc cgcggggatg acgatcggca cgaccgtgaa
     5821 gccctgcgcc tcgaggttgg cctgcaccgc ggcgccgtgc agcggcgcga cggtcgtgtc
     5881 ggagaccagc gccacggtgc cggcgcgacc gagtcgctgc tgcagctcac tgccgagccg
     5941 gccgatcgca ccgcgcccga tcagcaccgg gtagctgtgg ttctccgccg gtacgtggac
     6001 gtcgagcacc agagggcgtg gacgctgctt cgtcttggtc tcggtcatgc ggacccctcg
     6061 cgcagcgcgg ctaacactgt gcgaaccgca tcttgcggcg atcctccggc gtccacaacc
     6121 acgtttgcct cttcataagc agaccgacgt tgctgcgaca gcgcctcgat gcgcgctcgc
     6181 gttgcag
//
LOCUS       DASWNJ010001802         1542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_280217, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001802 DASWNJ010000000
VERSION     DASWNJ010001802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280217"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VGB85_30165"
     CDS             <1..897
                     /locus_tag="VGB85_30165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138403.1"
                     /translation="LATAFQRIGAAPGGSHAVRVRPGDYNVNTPLRATADHLVALVHA
                     TGGADDPEVKIFAAGAPAIAIDTNGALIIDRVRVQDGGNDGLSCMQGKAWLDRLTVDG
                     TAARGIATEGCDVRLRRSVLVDNAFTGAEISGGELQVENTFITRNGNTQSGGGGIYLA
                     GGATLDAVYTTWIENYGQAGTPFSVACDEDGGVEAVAVRNSVAVNKGLNTLCDGSQVS
                     DTAWSTDMPQGSNLAVTFADLPMYLTPDGTLIGVYRATPGTALDQLAKWQAGDPTIDF
                     DGDERPTGDSAPDFAGADRVGR"
     gene            1216..>1542
                     /locus_tag="VGB85_30170"
     CDS             1216..>1542
                     /locus_tag="VGB85_30170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138404.1"
                     /translation="MATMILLSACPPGGQGTSAETGDTESASTTETTGSGAEESVTEP
                     TTNQPTTPGTETEVTEGSMSGTVSQGGDTESTTDTTDPTSTTGPNPACTQNADCRDAA
                     LPFCEDE"
BASE COUNT          251 a          558 c          541 g          192 t
ORIGIN      
        1 ctggcgaccg cgttccagcg gatcggcgcc gcgcccggcg gctcgcacgc ggtccgcgtg
       61 cggccgggcg actacaacgt caacaccccg ctgcgcgcca ccgcggacca cctcgtcgcg
      121 ctcgtgcacg cgaccggcgg cgccgacgat cccgaggtca agatcttcgc cgccggggcc
      181 cccgcgatcg cgatcgacac caacggcgcg ctgatcatcg atcgcgtgcg cgtgcaggac
      241 ggcggcaacg acggcctgag ctgcatgcag ggcaaggcct ggctcgaccg cctgaccgtc
      301 gacggcaccg cggcgcgtgg catcgcgacc gagggctgcg acgtccgact gcgccgcagc
      361 gtgctggtcg acaacgcgtt caccggcgcc gagatcagcg gcggcgagct gcaggtcgag
      421 aacaccttca tcacccgcaa cggcaacacg cagagcggcg gcggcgggat ctacctcgcc
      481 ggcggggcga cgctcgacgc cgtctacacc acgtggatcg agaactacgg ccaggccggc
      541 acgcccttct cggtcgcctg cgacgaggac ggcggcgtcg aggcggtcgc cgtgcgcaac
      601 tcggtggcgg tcaacaaggg cctcaacacc ctgtgtgacg gctcgcaggt cagcgacacc
      661 gcgtggtcga ccgacatgcc gcagggcagc aacctcgccg tcacgttcgc cgatctgccg
      721 atgtacctga cgcccgacgg cacgctgatc ggggtgtatc gcgcgacccc ggggaccgcg
      781 ctcgatcagc tggccaagtg gcaggccggt gatcccacga tcgacttcga cggcgacgag
      841 cggcccaccg gcgacagcgc ccccgacttc gccggggccg atcgcgtcgg tcgctgaggg
      901 cgtgggtcgc cgtggtttgg gccgcggtct gggctgcagc gtgtcgttga cgtctacgag
      961 ctcgcgcgag gtgtgtcgac ggtggtgcat ggacgcgtcg aggacgcgga cgccatgtcg
     1021 tgccgggggg ccggcgcccc aggtgtcctc cgggccaggt ttgccggtgt gctccgccca
     1081 attgcgcagc gtcgcctctg gccccaaagc cggtttggca gcctcctgga caagcgtggg
     1141 gaggcaggga atactgcgcc gacccctgac ctaggacgta aaaccatgac gagagcgcac
     1201 cttcaaactg tttctgtggc cacgatgatc ctgctctccg cttgcccccc cggcggccag
     1261 ggcacctcgg cagagaccgg ggataccgag tccgcgagca ccacggagac gaccggaagc
     1321 ggcgccgagg agagcgtcac cgagccgacg accaaccagc cgacgacccc cgggaccgag
     1381 accgaggtga ccgagggcag catgtcgggg accgtgtccc agggcggcga caccgagagc
     1441 acgaccgaca cgaccgaccc gacgtcgacg accggcccga acccggcctg cacccagaac
     1501 gccgactgcc gcgacgcggc cctcccgttc tgtgaggacg ag
//
LOCUS       DASWNJ010001803         5914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_136783, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001803 DASWNJ010000000
VERSION     DASWNJ010001803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136783"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="VGB85_30175"
     CDS             <1..958
                     /locus_tag="VGB85_30175"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibrinogen-like YCDxxxxGGGW domain-containing
                     protein"
                     /protein_id="HEY0138405.1"
                     /translation="GEGCDLGANNSNSGACTLSCKLPACGDGFVQGGAGETCDDANLS
                     NNDACLNTCKLAKCGDGVVQQGVEQCDDGNLSNNDFCLNSCVQATCADGIKNGAETDV
                     DCGGACGKCGIGKVCAKNSECSLAACTNGTCQLTKTCKELKVADPALLDGDYTVDPDG
                     AGAIVPLKVHCDMDAGACGYTMIRFNDGNLGGDQTAYANKCSAVGMEVIVPRTKAHAT
                     AIVGWNGNVVPNLYNIFPKANGNTSIFNWTGRCQGQPCSFWMTDNADGNVSCGGYEPN
                     GDNNTAWRIYKVADGCGIQGGWNDANNAVAFTGHVICSPNDC"
     gene            complement(975..1745)
                     /locus_tag="VGB85_30180"
     CDS             complement(975..1745)
                     /locus_tag="VGB85_30180"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0138406.1"
                     /translation="MPEIVEVAGLRLEVAWHGPGPEREGTIVLLHEGLGCVAAWRDWP
                     AALAQASGRRVLTYSRRGYGGSQGRTPPWPLTYMHEEAQQVLPALLDAMAVRRAVLVG
                     HSDGGSIALIHASSGGARVCGVVTLAAHVFCEDLSVAAIAAARDEFLQGHLRERLARY
                     HGARVDDAFWGWNGAWLDPGFRAWNIEAFLPAITAPVHVMQGDADAYGTLAQVEAILR
                     GVRGPCSSQVLPGCGHAPWRERPAETTAAVLAMARAVL"
     gene            complement(1738..3330)
                     /locus_tag="VGB85_30185"
     CDS             complement(1738..3330)
                     /locus_tag="VGB85_30185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013799987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEY0138407.1"
                     /translation="MDGPYAQVEVGASPPRVEFPRRYNAAVDLLDRHVRAGRAAKVAY
                     VDDFGAVTYAQLLSRVNRVGQVLRGLGLAVEQRVLLLMHDTADFAACFLGAMKIGAVP
                     VPVNTLLTPADYLHMLVDSRARVLVVSDVLLPRVELALASVAAPPRVIVARSPLGGEA
                     GEHPRLEALCEGMADELAAADTTPDDVAFWLYSSGSTGAPKAAIHLHAHLVRTAVFYA
                     QGVLGLRADDVVYSAAKLFFAYGLGNALTFPLYVGATAVLRAERPTPALVLETLRSRG
                     VTVFGGVPTLFASLLAAPGLAPEGLALRVSLSAGEALPRHIGEQWRARLGSDILDGIG
                     STELLHIFLSNAPGDIRYGTSGRPVPGYPLKIVGEDGEAVADGEEGALWVRGTTAAAG
                     YFNQRARSLATFHGEWTYTGDRYVREADGRYVYSGREDDMLKVGGIWVAPFEVESALA
                     CHPAVLEAAVVGHADGDGLIKPRAFVVLKDPSAAGEALVEELRRFVKGQLAPYKYPRW
                     IEFVGELPKTATGKIQRFRLRA"
     gene            complement(3333..3989)
                     /locus_tag="VGB85_30190"
     CDS             complement(3333..3989)
                     /locus_tag="VGB85_30190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138408.1"
                     /translation="MLFVGDGKHDIGGPDGFSDEPFPARGVVPNLSARVAALDGERSV
                     ALRWKDLVRFSPTARGYPAKIKLAQLLAERKWGLGGAVCVVDEDNDPSRRTLSGTQGS
                     ASCPVVCGVAVRSIEAWTLGAPTALAAALGTTPEKVQHACPSARVEDLYEGSGKPELR
                     SKQLLRKLATELGRCDDSLELREEVARRTDPDKLCRTCSQGFAPFAAGLREAFGRPGV
                     "
     gene            complement(3995..5173)
                     /locus_tag="VGB85_30195"
     CDS             complement(3995..5173)
                     /locus_tag="VGB85_30195"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0138409.1"
                     /translation="MLRTFHVENFKCLRDTTVELEPFTVLIGPNDSGKTSILDALRLI
                     GQTFVKPVDEVFSGQNRAEVLAWRRQAGTELGWQLRFAEGKQEFNYRYSWPSGKERAD
                     SPRVAIVEKDGTKTHLGCKSWNGMSYVSASFFSQHSSADTGLRTVLCYPNPSAGIFIK
                     EVQPFTSVLADLRRYRLDPDALRTPAVPEPDAVLTPSGDNLVAVLDRLISGPDRTAIV
                     NLEQKLHEAIPTLRGIALRTADGKTGTKTLEFVLDGPGKAFTTIPCAHASDGAMLLTA
                     FLALAYSEQPGMLLIEEPENGLHPSRLRAVVEMLRKLSTGEVGTRPRQVILTTHSPLL
                     LNYTKPEEVRIVRRDPATGTHVTPLTAVPDIHKLLEEFAVGELWYLLGEEALVEGKKP
                     "
     gene            5255..>5914
                     /locus_tag="VGB85_30200"
     CDS             5255..>5914
                     /locus_tag="VGB85_30200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138410.1"
                     /translation="MAKAGDEIKELQRRLRHLARLDGVVVRTADGPVLLRPTGVDPFA
                     LAAACHAGRPPLAAVLAAVPCDGEPQRLTPTLVSRAHRDLRALELSPWLATVRAAPRH
                     FRSFGALDDRWLATRAARIDRLIHALEPASPDPASDPVHDLVADLHGPDAAAALADWL
                     ERSAGLGARRRREAGRRLEGLERRLLDPSTLPREPPALSAMLDAAAAELLHLATLPGR
                     AA"
BASE COUNT         1009 a         2291 c         1794 g          820 t
ORIGIN      
        1 cggcgagggc tgcgacctcg gcgcgaacaa cagcaacagc ggcgcgtgca ccctctcgtg
       61 caagctcccg gcctgcggcg acggcttcgt acagggcggc gccggcgaga cctgcgacga
      121 cgccaacctc agcaacaacg acgcctgcct caacacctgc aagctcgcca agtgcggcga
      181 cggtgtcgtg cagcagggcg tcgagcagtg cgacgacggc aacctcagca acaacgactt
      241 ctgcctcaac tcgtgcgtgc aggcgacctg cgccgacggc atcaagaacg gcgccgagac
      301 cgacgtcgac tgcggcggcg cgtgtggcaa gtgcgggatc ggcaaggtgt gcgcgaagaa
      361 cagcgagtgc agcctcgccg cctgcaccaa cggcacctgc cagctgacga agacctgcaa
      421 ggagctcaag gtcgccgacc ccgcgctgct cgacggcgac tacaccgtcg accccgacgg
      481 cgccggcgcg atcgtccccc tcaaggtcca ctgcgacatg gacgctggcg cctgcggcta
      541 cacgatgatc cgcttcaacg acggcaacct cggcggcgac cagaccgcct acgccaacaa
      601 gtgcagcgcg gtcggcatgg aggtgatcgt gccgcgcacc aaggcccacg cgaccgcgat
      661 cgtcgggtgg aacggcaacg tcgtgcccaa cctctacaac atcttcccca aggccaacgg
      721 caacaccagc atcttcaact ggaccggccg ctgccagggc cagccttgct ccttctggat
      781 gaccgacaac gccgacggca acgtgtcctg cggcggttac gagcccaacg gcgacaacaa
      841 caccgcgtgg cggatctaca aggtcgccga cggctgcggg atccagggcg ggtggaacga
      901 cgcgaacaac gcggtcgcct tcaccggcca cgtcatctgc tcgcccaacg actgctagac
      961 gcgcatgcgc tgattcacag caccgcccgc gccatggcga gcaccgcggc cgtcgtctcc
     1021 gccggccgct cgcgccacgg tgcgtgcccg cagcccggca gcacctgcga gctgcacggc
     1081 ccccgcacgc cccgcaagat cgcctcgacc tgcgcgagcg tgccgtaggc gtcggcgtcc
     1141 ccctgcatca cgtgcaccgg cgccgtgatc gcgggcaaga acgcctcgat gttccacgca
     1201 cgaaaccccg ggtcgagcca ggcgccgttc cagccccaga acgcatcgtc gacgcgcgcc
     1261 ccgtgatacc gcgccagtcg ctcgcgcaga tgaccctgca aaaactcgtc gcgcgccgct
     1321 gcgatcgcgg cgaccgaaag atcttcacag aacacgtgcg ccgcgagcgt gacgacccca
     1381 catacccgcg cccccccgct gctcgcgtgg atcagcgcga tcgatccacc gtcgctgtgc
     1441 ccgacgagca ccgcccggcg caccgccatc gcgtcgagca gcgccggcag gacctgctgc
     1501 gcctcctcat gcatgtacgt cagcggccac ggcggcgtcc gcccctgcga gccaccatac
     1561 ccgcgccggc tgtatgtcag cacccggcgc ccgctcgcct gcgccagcgc ggccggccag
     1621 tcgcgccacg cggccacgca gccgagccct tcgtgcagca gcacgatcgt gccctcccgc
     1681 tcgggtccgg gcccgtgcca cgcgacctcg aggcgaagcc cggcgacctc gacgatctca
     1741 ggcacgcagg cgaaaccgct ggatcttccc ggtggccgtc ttcggcagct cgccgacgaa
     1801 ctcgatccac cgcggatatt tataaggtgc gagctgcccc ttcacgaacc gccgcagctc
     1861 ttccaccaac gcctcccccg cggccgaagg atccttgagc accacgaacg cgcgcggttt
     1921 aattaaacca tcgccgtcgg cgtgcccgac cacggccgcc tcgagcaccg ccgggtggca
     1981 cgcgagcgcc gactcgacct cgaagggcgc gacccagatc ccgccgacct tgagcatgtc
     2041 gtcctcgcgc ccggaataca cgtaccgccc gtccgcctcg cgcacgtagc ggtcccccgt
     2101 atatgtccac tcgccatgaa aagtcgccag cgaccgcgcc cgctgattaa aatagccggc
     2161 cgccgcggtc gtgccgcgca cccacagcgc cccctcctcg ccgtccgcca ccgcctcccc
     2221 atcctccccg acaattttta agggatatcc ggggaccggc cggccgctcg taccgtaacg
     2281 aatgtccccg ggcgcgttcg acaaaaagat atgcagcagc tcggtcgacc cgatgccatc
     2341 caaaatatcg ctgccgagcc gcgcccgcca ctgctcgccg atgtgccgcg gcaacgcctc
     2401 gcccgccgac agcgacaccc gcagcgccag cccctcgggc gccagccccg gtgccgcgag
     2461 caacgaggcg aacagcgtcg gcaccccgcc gaacaccgtg accccgcgcg accgcagcgt
     2521 ctccagcacc agcgccggcg tcggccgctc ggcacgtaac accgcggtcg ccccgacgta
     2581 cagcggaaaa gtcagcgcat tgccgagccc gtaggcgaag aacagcttgg ccgccgaata
     2641 cacgacatcg tcggcgcgca gcccgagcac gccctgcgcg taaaacaccg ccgtgcgcac
     2701 caggtgcgcg tgcaggtgga tcgccgcctt cggcgcgccg gtcgacccgg acgagtacag
     2761 ccagaacgcg acgtcgtccg gtgtcgtatc tgccgccgcg agctcgtccg ccataccctc
     2821 gcacagcgcc tcgagccgcg ggtgctcccc cgcctccccg ccgagcggcg agcgagcgac
     2881 gatcacgcgc ggaggcgcgg ccacgctggc gagcgccagc tcgacgcgcg gcagcaacac
     2941 atcgctgacg acgagcaccc gcgcccggct gtcgaccagc atatgcagat agtccgcggg
     3001 cgtcagcagc gtattgaccg gcaccggcac cgccccgatc ttcattgccc cgaggaagca
     3061 ggccgcgaag tcggccgtat cgtgcatcag caacagcacc cgctgctcga ccgccagccc
     3121 gagcccgcgc agcacctgcc cgacccgatt cacgcgcgac agcaattgcg catatgtgac
     3181 cgccccgaag tcgtcgacat acgcgacctt cgccgcccga cccgcccgca cgtgccggtc
     3241 gagcaggtcg accgccgcgt tgtagcgccg cggaaactcg acccgcggcg gactcgcccc
     3301 gacctcgacc tgcgcgtacg gcccgtccat gctcaaactc ctggcctccc gaacgcctcg
     3361 cgcaaacccg cggcgaacgg cgcgaacccc tgcgaacatg tgcggcacag cttgtcgggg
     3421 tcggtgcgtc gcgccacctc ctcacgcagc tcgaggctgt catcgcaccg cccgagctcc
     3481 gtggcgagct tgcgcaacag ctgcttggac cgcagctcgg gcttgccgct gccctcgtac
     3541 aggtcctcga ctcgcgcgga gggacaggcg tgctgcacct tttctggagt cgtgccgagc
     3601 gcggcggcga gcgccgtggg tgcaccgagg gtccaggcct cgatcgaccg caccgcgacc
     3661 ccgcagacga ccgggcacga cgccgagccc tgcgtcccgc tcagcgtgcg acgcgaggga
     3721 tcattatctt catcaacgac acaaacggct ccgccgagac cccatttacg ttcagcgagc
     3781 agttgggcga gcttgatctt cgccgggtag ccgcgcgccg tcggcgagaa gcggaccagg
     3841 tccttccacc gcaacgcaac ggaccgctcg ccgtcgagcg cggcgacgcg cgcagagaga
     3901 ttcggcacca ccccgcgagc cggaaacggc tcgtccgaga acccgtccgg accgccgatg
     3961 tcatgcttgc cgtctccgac gaacagcacg cgcctcacgg cttcttgccc tccaccagcg
     4021 cctcttcacc gagcagatac cacagctcgc cgaccgcaaa ctcttcaaga agcttgtgga
     4081 tatcgggcac cgccgtcagc ggcgtcacat gcgtccccgt ggccggatcg cggcgaacga
     4141 tcctgacctc ctcgggcttt gtatagttga gcaacagcgg gctgtgcgtc gtcaagatga
     4201 cctgccgcgg ccgcgtcccg acctcgcccg tgctcagctt tcgcaacatt tctacgacgg
     4261 cccgcagccg cgacggatgc agcccgttct ccggctcctc aattaatagc atgcccggct
     4321 gctcgctgta ggcgagcgcc aggaaggccg tcagcaacat cgcaccatcc gacgcatgcg
     4381 cacacggaat ggtcgtaaaa gccttgccag gtccgtcgag cacgaactcg agggtcttcg
     4441 tacccgtctt gccgtccgcg gttcgcaacg cgatgccccg gagcgtcggg atcgcctcgt
     4501 gaagcttttg ctcgaggttg acgatcgcag tacggtccgg acctgagatc aaacgatcca
     4561 ggaccgcgac caaattatca cccgacggcg tcagtaccgc gtccggctcg gggaccgcag
     4621 gggtgcgcaa tgcgtcagga tcaagtcggt atcgccgcaa atctgcgagg accgaagtaa
     4681 atggttgtac ttcctttata aaaattccgg cagacggatt ggggtaacac aggacagtac
     4741 gaagcccagt atccgcgctt gaatgctgcg agaagaagga tgccgacacg tatgacatgc
     4801 cgttccagga tttacatccg agatgcgtct ttgttccgtc cttctcgacg atcgccacgc
     4861 gaggagagtc ggcgcgttcc ttacccgagg gccaagaata tcgataattg aattcttgct
     4921 tcccttcggc gaagcgaagc tgccagccca attccgtccc cgcctgccgc cgccacgcca
     4981 gcacctccgc gcggttctgc ccggaaaaca cctcgtccac cggcttaaca aacgtctgcc
     5041 cgatcagccg cagcgcgtcg aggatgctcg tcttcccgct gtcgttcggc ccgatcagca
     5101 cggtgaaggg ctcgagctcg accgtcgtgt cgcgcaggca cttgaagttc tcgacgtgga
     5161 aggtccggag catgcgcgcg ctcagggtat ccgaccgggc gcgcgcggag caagcatctg
     5221 cccgcccgcg gatcggctat cctcgccgcc tgggatggcc aaggccggag acgagatcaa
     5281 agagctgcag cgccgccttc gtcacctcgc gcggctcgac ggtgtcgtcg tgcgcaccgc
     5341 cgacgggccc gtgctgctgc gaccgaccgg tgtcgacccc ttcgcgctgg ccgcagcgtg
     5401 ccacgcgggt cgccctcctc tcgcggccgt gctcgccgcg gtcccctgcg acggcgagcc
     5461 ccagcgcctg acgccgacgc tggtctcgcg ggcgcaccgc gacctgcgtg ccctcgagct
     5521 atcgccgtgg ctggcgaccg tccgcgcggc tccccgacac tttcgcagct tcggtgcgct
     5581 cgacgatcgc tggctcgcca cgcgggccgc ccggatcgac cggctgatcc acgccctcga
     5641 gcccgcgtca cccgaccctg cgtccgaccc cgtgcacgac ctcgtcgccg acctccacgg
     5701 gcccgacgcc gccgccgcgc tcgccgactg gctcgagcgc agcgccgggc tcggcgctcg
     5761 ccgccgccgc gaggccgggc ggcgcctcga gggtctcgag cgccgcctgc tcgacccgtc
     5821 cacgctcccg cgcgagccgc ccgcgctctc cgcgatgctc gacgccgccg ccgccgagct
     5881 gctccacctg gccaccctcc ccggccgcgc cgcc
//
LOCUS       DASWNJ010001804         2986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_384007, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001804 DASWNJ010000000
VERSION     DASWNJ010001804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384007"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VGB85_30205"
     CDS             complement(<1..547)
                     /locus_tag="VGB85_30205"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0138411.1"
                     /translation="MLSDVNQDSVVGALLDGRYRIIKELGHGGMGNVYLAEETRLRRR
                     CALKILHPQLAQERTYVERFLREAQMIAQLEHANIVDIYAYGEEPSGIVWFAMELLTG
                     EDLDARLKARAERPFGTHEACAWAVQIARAVGVVHQAGLIHRDLKTSNVFLARRRDGE
                     EIVKLLDFGIARPEEGSELTAT"
     gene            702..1607
                     /locus_tag="VGB85_30210"
     CDS             702..1607
                     /locus_tag="VGB85_30210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138412.1"
                     /translation="MVGYVARTRSPATSTLHCMMVAAIIASSLPPGVVSAAPAGQPTA
                     SDLAAFDRGFRAGQDEFNRHNYLEAAKVWTTAAASLPETEEHKDNRRAIYEYIAEAYE
                     KAVELKPDEATLREGLVTLDAYASAFVAAHPADTLPERVTKARADFRSRLDEIEAERR
                     RREEPPEVAPAPAPVVAPEPVRPPPKPWKGLAIGGGVAVAGGAAMLAMFAVGYTRAKS
                     AQSRFDAPENACDPADPTGMCADINSQGKTANSVAVAGLVAAPLLLGAGIGMLVIATR
                     RKANRTALVPMFGKNIAGVVWHTRF"
     gene            complement(1625..1918)
                     /locus_tag="VGB85_30215"
     CDS             complement(1625..1918)
                     /locus_tag="VGB85_30215"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY0138413.1"
                     /translation="MAFFFARLTPLRPDFPADMTPAEGAVMQQHGAFLGEQLARGVLV
                     AAAPVFDPGGVFGMAIFEADSLDVVQSLLASDPANAVGRYEVLPMGPTIARAR"
     gene            complement(1967..2446)
                     /locus_tag="VGB85_30220"
     CDS             complement(1967..2446)
                     /locus_tag="VGB85_30220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138414.1"
                     /translation="MAVPRARTLFLLGLLAVAPLSGCSRVVVLTASEQASFGTRTFAA
                     PLAVTLQAAREALEGLDYSIAAVDSRGGKITTGRKLKGVQASIGLFGRSAEPLYQQFV
                     LQLQPVGAAQTRVIAVPHKYAGERDISRERTWFLDGDQGLHHEWRSMFNSIQANLSQ"
     gene            2651..>2986
                     /locus_tag="VGB85_30225"
     CDS             2651..>2986
                     /locus_tag="VGB85_30225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138415.1"
                     /translation="MSQVIPVRCPHCGAGLQVDPRAESATCAYCGTTSLLRNKGTVVT
                     NGTPVITVGPGRAKTALLVTVAVGVLGVVLAMYFAGPPSTPAPAPGRAQDPVEFVARP
                     EPAAPATVDD"
BASE COUNT          492 a         1060 c          989 g          445 t
ORIGIN      
        1 cggtcgcggt cagctcggag ccctcctcgg gccgcgcgat cccgaaatcc aggagcttga
       61 cgatctcctc cccgtcgcga cgtcgggcca ggaacacgtt cgaggtcttg aggtcgcggt
      121 ggatcagccc cgcctggtgc accacgccca cggcgcgtgc gatctggacc gcccacgcgc
      181 aagcctcgtg ggtgccgaac ggccgctctg cccgggcctt gaggcgcgca tcgaggtcct
      241 ccccggtgag cagctccatg gcgaaccaca cgatgcccga gggctcctcg ccatatgcgt
      301 agatatcgac gatattggcg tgctcgagct gcgcgatcat ctgcgcttcg cgcaggaagc
      361 gttcgacgta cgttcgctcc tgcgcgagct gagggtgcaa tatcttcaat gcgcagcgac
      421 ggcgcagccg ggtctcctcg gcgagataca cgttacccat accgccatgg cccagctcct
      481 tgatgatgcg ataacgccca tcgagcagtg ctccgacgac ggagtcctga tttacgtccg
      541 ataacacagc agctctcact tctacacgag cccccgaacg gcttcaacag gccgatggcg
      601 gctcattgcg cgaaaacgcc gcgcccgggc ccggggcctc tgcgcagttc gtgccggcga
      661 aaggcgaacc tttgctgcat cgcctgtgat agaagagggc catggttgga tacgtcgcgc
      721 gaacgcgctc gccggcgacg agcacgctgc actgtatgat ggtggcagcg atcatcgctt
      781 cttcactacc acctggcgtc gtgagcgccg cgcccgcggg tcagccgacg gcgtccgacc
      841 tcgcggcctt cgatcgcggg tttcgggccg gtcaggacga gttcaaccgg cacaactacc
      901 tcgaggctgc caaggtctgg acgaccgcgg ccgcctcgct gcccgagacc gaggagcata
      961 aggacaaccg gcgggcgatc tacgagtaca tcgcagaggc ctacgagaag gccgtcgagc
     1021 tcaagcccga cgaggcgacc ctgcgcgaag ggctcgtgac gctggacgcc tatgcgagcg
     1081 cgttcgtggc ggcgcatcca gcggacacgc tgccggagcg cgtgacaaag gcacgagccg
     1141 actttcgcag ccggctcgac gagatcgagg ccgagcgacg acggcgcgag gagcccccgg
     1201 aggtcgcccc cgcccccgcc cctgtcgtcg cgccggagcc ggtgcgtccg cccccgaaac
     1261 catggaaggg cctcgctatc ggtggaggtg tcgccgtcgc cggcggagcc gcgatgcttg
     1321 cgatgttcgc ggtgggatat acccgcgcca agtcggccca gtccagattc gacgcccccg
     1381 agaacgcctg cgatcccgcc gacccgaccg gcatgtgtgc cgatatcaac agtcagggta
     1441 aaaccgccaa cagcgtcgcc gtcgctgggc tcgtggccgc gccgctcctg ctcggcgccg
     1501 gcatcggaat gctcgtgatc gccacccgac gcaaggccaa ccggacagcg ctggtcccga
     1561 tgttcggcaa gaacatcgct ggcgtggtgt ggcataccag gttctgagcc ggagtctgcc
     1621 gcagctaccg cgcgcgagcg atcgtcggtc ccatcggcag gacctcgtag cgaccgaccg
     1681 cgttcgccgg gtccgacgcg agcaacgact gcacgacatc gagcgagtct gcctcgaaga
     1741 tcgccatccc gaacacgccg cccgggtcga acaccggcgc ggccgccacg agcacgcccc
     1801 gcgccagctg ctcgccgagg aacgcgccat gttgctgcat cacggccccc tcggcgggcg
     1861 tcatgtctgc ggggaagtcg gggcgaagcg gcgtgagacg ggcaaagaag aacgccatgc
     1921 cggagcatcg ccgatccgac gcgcgaacgc gagcccaaac gtcgatctac tggctcaggt
     1981 tggcctggat cgagttgaac atcgatcgcc actcgtggtg cagcccctgg tccccgtcga
     2041 gaaaccacgt gcgctcgcgc gagatgtcgc gctcgccggc atacttgtgc gggacggcga
     2101 tcacgcgggt ctgggcggcg ccgaccggct ggagttggag gacaaattgc tggtacagcg
     2161 gctccgcgct gcgaccgaac agcccgatcg acgcctgcac gcccttgagc ttgcgcccgg
     2221 tcgtgatctt cccgcccctg ctgtcgaccg cggcgatcga gtagtcgagg ccctccagcg
     2281 cctcgcgggc ggcctgcaag gtgacggcga gcggcgcggc gaaggtgcgg gtcccgaagc
     2341 tcgcctgttc actggcggtc agcacgacca cgcgtgagca gccggacagc ggcgcgacgg
     2401 ccagcaggcc caggaggaac aacgtacgtg cgcgcggaac cgccatggcg acggcatcct
     2461 cgcatcgccc gacgacagcg tccagcgtcg tcgacgacat gcgatgccgc tgcatgcccg
     2521 cctcgtgccc gtgtgtctcc cggtcgatcg tccgccctgg atctcacgcc ggccgatcgc
     2581 gcgatcgctg cgccgggcgc ccgcgctggt acgcgtcacg cgagcagcca attttggcta
     2641 tgctcgcggc ttgtcgcaag tcatcccagt acgctgcccg cactgcggcg ccggtctgca
     2701 agtcgacccg cgagcagaat ctgccacctg cgcctattgt ggtacgacct cgctgctgcg
     2761 caacaagggc acggtggtga caaacggaac acccgtgatc accgtcggcc cgggtcgcgc
     2821 aaagacggcg ctgctcgtca cggtcgcggt cggtgtgctc ggggtggtgc tcgccatgta
     2881 cttcgcgggc ccgccgtcca ccccggcgcc cgccccgggg cgcgcgcaag accccgtcga
     2941 gttcgtcgcc aggcccgagc cggccgcccc cgcgactgtc gacgac
//
LOCUS       DASWNJ010001805         2080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_32060, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001805 DASWNJ010000000
VERSION     DASWNJ010001805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32060"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VGB85_30230"
     CDS             complement(<1..568)
                     /locus_tag="VGB85_30230"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0138416.1"
                     /translation="MIEEGSSAEPDWPGYLAAFHAERAGITEDVLEHACDDGGRTPYD
                     WAADALREGLSDDASARVLDLACGSAPMSLRVRGHDYLGMDLSVAELARARARALPVT
                     AADAARLPLADASVDAVVMSMALMLVPLRPALAEIRRVLRPGGVFVATMPHNRPMPTG
                     DWLRYARLCVAMRHPGLSYPNDKPLRDAR"
     gene            complement(697..1092)
                     /locus_tag="VGB85_30235"
     CDS             complement(697..1092)
                     /locus_tag="VGB85_30235"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEY0138417.1"
                     /translation="MDIALTTHTDLPFAETVEQVREALAAQGFGVLTEIDVQATMKTK
                     LDIDLEPYLILGACNPPLAHQALTAEPEIGLLLPCNVTVRTEAGRTVVQAMNPQLMVS
                     VVDRPALQAVADEAALRLQAALDSLHATS"
     gene            1180..>2080
                     /locus_tag="VGB85_30240"
     CDS             1180..>2080
                     /locus_tag="VGB85_30240"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY0138418.1"
                     /translation="MTTATTAPPTTSPPAESRPGPLARLGLLTATRRRAVFIAWALLV
                     VALGAFAPRVESALAGAGWEASGSQSVQTRDVVQREFAGLSSAALMVVLHREDGPVLE
                     GPGAAAVQQATALLEADPRVSSVIAPQPGLSVSQDGRTAIVQGGAATPNTNELVRAAD
                     DLKEPLRELGGDGVTVALTGSSGLWSDFNEANLNAMLKSELYSWPVTLAILVLAFGSL
                     VAAGLPLLLTITGLVAAAGSLFLAAQLGDISIWAMNFAMMFALALGIDYALFIVVRFR
                     AARAKADSVEQAVAETLDTAGKAV"
BASE COUNT          285 a          758 c          728 g          309 t
ORIGIN      
        1 cgcgggcatc gcgcagcggc ttgtcgttgg ggtagctgag gccgggatgg cgcatcgcca
       61 cgcagagcct tgcgtagcgg agccagtcac cggtcggcat cggccggttg tgcggcatcg
      121 tcgccacgaa cacccctccg gggcgaagga cccgccggat ctccgcgagc gccgggcgta
      181 gcgggacgag catcagcgcc atcgacatga ccacggcatc gacggacgcg tccgcgagcg
      241 gcaggcgcgc cgcgtcggcc gccgtgaccg gaagagcacg cgcgcgggcc cgagcgagct
      301 cggcgacgga caggtccatc ccgaggtagt cgtgccccct gacccgcagg gacatcggag
      361 cgctcccgca cgcgaggtcc aacacccgag ccgacgcgtc gtcggacagc ccctcgcgca
      421 aggcgtcggc ggcccagtcg tagggcgtgc ggccgccgtc gtcacatgcg tgctcgagca
      481 cgtcttcggt gatgccggcc cgctcggcgt ggaatgcggc gaggtacccc ggccagtcgg
      541 gctcggcgga cgagccttcc tcgatcaacc gcttccccct cgttggcaag actgcctgca
      601 cgactcgcgg cccctgtcac aggtcgtgca ccctgcgatg tccgcctatc gacgtgcagc
      661 ttgcgccttg acccgacgag cccccctggt cgctggtcag ctggtggcgt gcagtgagtc
      721 cagggctgcc tgcagcctga gcgctgcctc gtcggcgacc gcctgcagtg cgggccggtc
      781 gacgacggag accatcagct gcgggttcat ggcctggacc accgtccgcc ctgcctcggt
      841 gcgcacggtg acgttgcacg gcagcagcaa cccgatctcg ggttccgcgg tcagcgcttg
      901 atgggccagc ggcgggttgc acgcacccag gatcaggtac ggctcgaggt cgatgtcgag
      961 cttggtcttc atcgtggcct gcacgtcgat ctcggtcagg acgccgaagc cctgtgccgc
     1021 gagcgcctcg cgcacctgct cgacggtctc ggcgaacggc aggtcggtgt gggtggtgag
     1081 cgcgatgtcc atgcctacga ccgtagtacc cccgggggta cttgacaagt acccccgggg
     1141 gtatcgagac tggctgtcgt cactcgatga aggagcgcca tgacgaccgc cacgaccgca
     1201 ccgccgacca cctcgccgcc cgccgagtcg cggcccggac cgctcgcccg gctgggcttg
     1261 ctgaccgcca cccggcgccg cgccgtgttc atcgcctggg cgctgctcgt agtggcgctg
     1321 ggggcgttcg cgccccgcgt cgagtccgcg ctcgccggcg ccggctggga ggcgtcgggg
     1381 tcgcagtcgg tgcagacccg cgacgtcgtg cagcgcgagt tcgccgggct cagcagcgca
     1441 gccctgatgg tcgtgctgca ccgcgaggac ggtccggtgc tcgaaggacc cggcgccgcc
     1501 gccgtccagc aggcgaccgc gctgctggag gccgaccccc gcgtctccag cgtcatcgcg
     1561 ccccagccgg gcttgtcggt gtcgcaggac ggccgcaccg ccatcgtgca aggcggagcc
     1621 gcgacgccca acaccaacga gctggtgcgc gcagccgacg acctcaagga gccgctgcgt
     1681 gagctcggcg gggacggcgt caccgtcgcg ctgaccggct cgtccgggct gtggagcgac
     1741 ttcaacgagg ccaacctgaa tgcgatgctc aagagcgagc tctactcgtg gcctgtcacg
     1801 ctggcgatcc tggtgctcgc gttcggctcc ctggtggccg ccgggctgcc gctgctgctc
     1861 accatcaccg gcctggtcgc cgcggccggc tcgctgttcc tggccgcgca actcggcgac
     1921 atcagcatct gggcgatgaa cttcgccatg atgttcgccc tggccctggg catcgactac
     1981 gccctgttca tcgtcgtgcg cttccgagca gcccgagcca aggccgacag cgtcgaacag
     2041 gccgtcgcgg agaccctcga caccgccggc aaggccgtcc
//
LOCUS       DASWNJ010001806         3261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_384019, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001806 DASWNJ010000000
VERSION     DASWNJ010001806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384019"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VGB85_30245"
     CDS             <1..369
                     /locus_tag="VGB85_30245"
                     /inference="COORDINATES: protein motif:HMM:NF013555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HEY0138419.1"
                     /translation="DVPCPKCGSKLNLRSGRRGPWLGCSKFPKCRGRLAWTALEEPVQ
                     AELTKLLEDHEKLHPKTVLRRHDGSVIPEGTPISTLLIPGGIAELEIHPDAFAALKGL
                     PAPPAPIAALPAAVPGPASN"
     gene            complement(406..921)
                     /locus_tag="VGB85_30250"
     CDS             complement(406..921)
                     /locus_tag="VGB85_30250"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEY0138420.1"
                     /translation="MTRPATRVLFVCHANMVRSPLAEGVFRHMTAARGWSDHFVIASA
                     GVSAFEGMPPDPGSVAVAAAHGITLTSRSRQLTRADLYDHHHVLLADRHVQSQIRRLM
                     GGSAFGELSGHAGSGARVRLLAALADPHAEGGRLDVADPVRGGPEGYQRMYQQVERAC
                     AALLRELAPGA"
     gene            complement(932..1675)
                     /locus_tag="VGB85_30255"
     CDS             complement(932..1675)
                     /locus_tag="VGB85_30255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138421.1"
                     /translation="MRSRLVRLVLATATVCTAAACQTPTSKDAPAEVKSAAVPTPVTP
                     DAKTSDTAGTAGTAGTAGTATAGTVGAPGTAGDPTVTASAGEQTPTTGSETAGMGGTG
                     TAGTAGTAGDPAAAPVDKAKLFTEVKAKKTSDARAKKALEEAEAGGATVRELAEASNA
                     RGVALQNAGEGDRAKAAFEWARDKDPTYPDASFNLAKQTANAGEVPETVAHLKEVHKR
                     GGKALLKQVGFDPTFEIVKDDPEITKFVK"
     gene            1751..>3261
                     /locus_tag="VGB85_30260"
     CDS             1751..>3261
                     /locus_tag="VGB85_30260"
                     /inference="COORDINATES: protein motif:HMM:NF040196.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HEY0138422.1"
                     /translation="MSLPFTIYLALAAPASDLETKVQKIFEDNCTACHGDGGEAPNLE
                     GAPSRLTSMKSKETGKPMVTPGDPDKSYVFAKILGKNIAGDVMPLGGDPLSAAQKKAV
                     KDWIASLAPAAEASGAPPTTSTGTKAGSGDASKRVAELEPKVQKIFADQCISCHGTGD
                     DLVLSGSPEGLVRVQSKPTKKPLIVAGDPDASYLIAKVTGAKGIEGDPMPLGDTMPKQ
                     DVEVLRQYIVALGDRDKAGAGGAAMTGDPSSGPNLPVGDTGVVAGDGPTDSEPAGSDG
                     EPAKPSRPPFHGVFQINLPSTSTLGKRTFEFRIDHRFGRVGAERGAFGLDAGANISYG
                     FAYGILDGWDVLLRRSNSRKGYELGTKYIPIRQEAGKPLSFGGYASIEYLRDFPSNTA
                     NPWAGNFQLMLSRLWFERWSTMLLVGYSLRTDHKAAPTVDFDGNGGAAPVRVRDKRGT
                     LGVGLASTVWIGEKKRWGIDMEYWLPIPSNKFYFNGGNADPNGSNIGSWSLGGS"
BASE COUNT          468 a         1194 c         1127 g          472 t
ORIGIN      
        1 gatgtgccgt gcccgaagtg cggctcgaag ctcaacctcc gctccggtcg ccgcggcccc
       61 tggctcggct gctcgaagtt cccgaagtgc cgcggccgcc tggcctggac ggcgctcgag
      121 gagccggtcc aggccgagct caccaagctg ctcgaggacc acgagaagct gcacccgaag
      181 acggtcctgc gccgccacga cggcagcgtg atccccgagg gcaccccgat ctcgaccctg
      241 ctgatccccg gcggtatcgc cgagctcgag atccacccgg acgccttcgc ggcgctcaag
      301 ggcctgcccg cgcccccggc cccgatcgcg gccctgccgg ccgccgtgcc cggcccggcg
      361 agcaactgag acaggaccag tccgcgagga caggtcgatg tgtcgtcacg ccccgggcgc
      421 cagctcgcgc agcagcgccg cgcaggcgcg ctcgacctgc tgatacatgc gctggtagcc
      481 ctcggggccg ccgcgcaccg ggtcggcgac gtcgaggcgg ccgccctctg cgtgtgggtc
      541 ggcgagggcc gccagcaggc gcacgcgggc gccgctgccg gcgtgcccgc tcagctcgcc
      601 gaacgccgag ccgcccatca ggcgccggat ctgcgactgc acgtggcggt cggcgagcag
      661 cacgtggtgg tggtcgtaga ggtcggcgcg cgtcagctgg cgcgagcggc tcgtcagcgt
      721 gatgccatgg gcggccgcga cggcgacgct gccggggtcc gggggcatgc cctcgaaggc
      781 cgagacgccc gcgctggcga tcacgaagtg gtcgctccac ccgcgggcgg cggtcatgtg
      841 gcgaaacacg ccctcggcca gcggcgagcg gaccatgttc gcgtggcaca cgaacagcac
      901 ccgcgtggcc ggacgcgtca tgaacagcct tttacttcac gaacttcgtg atctcggggt
      961 cgtccttgac gatctcgaag gtggggtcga agccgacctg cttgagcagc gccttgccgc
     1021 cgcgcttgtg tacctccttg aggtgcgcga cggtctcggg gacctcgccc gcgttggctg
     1081 tctgcttggc caagttgaag ctcgcgtccg gataggtcgg gtccttgtcg cgcgcccact
     1141 cgaacgccgc cttcgcgcgg tcgccctcgc ccgcgttctg cagcgccacg ccgcgcgcgt
     1201 tgctcgcctc cgcgagctcg cgcaccgtcg cgccgccggc ctcggcctcc tcgagcgcct
     1261 tcttcgcgcg cgcgtcgctg gtcttcttcg ccttcacctc ggtgaacagc ttggccttgt
     1321 cgaccggcgc ggccgcgggg tcacccgcgg tgcccgcggt gcccgcggtg cccgtgccgc
     1381 ccatgcctgc ggtctcggag ccggtcgtcg gcgtctgctc gcccgcggac gcggtgaccg
     1441 tcggatcacc cgcggtgccc ggcgctccga ccgtgcccgc agtggcagtg ccggcagtgc
     1501 cggcggtgcc ggcggtgccg gcggtgtcgc tggtcttcgc gtcgggtgtc accggcgtcg
     1561 ggaccgcggc ggatttgacc tccgcgggcg catccttgct ggtgggcgtt tggcaggctg
     1621 cggccgtaca aacggtcgcg gtggcgagga cgagccggac gaggcgcgag cgcatgcggc
     1681 gataatatcc gagccagcgg ggctgcgcat tgtggtaaca ctcacacctg ccgtacggta
     1741 ggccctgccc atgtcgctgc ccttcacgat ctacctcgca ctcgcagctc cggcctctga
     1801 tctcgagacc aaggtccaga agatcttcga ggacaattgc accgcatgtc acggcgacgg
     1861 cggtgaggcg ccgaacctcg agggtgcgcc gtcgcggctc acctcgatga agtcgaagga
     1921 gaccggcaag ccgatggtca cccccggtga ccccgacaag agctacgtgt tcgccaagat
     1981 cctcggcaag aacatcgccg gcgacgtgat gccgctcggc ggtgacccgc tgtcggccgc
     2041 gcagaagaag gccgtcaagg actggatcgc cagcctcgcc ccggccgccg aggccagcgg
     2101 cgccccaccg accacgagca cgggcacgaa ggcaggctcg ggcgacgcca gcaagcgggt
     2161 cgccgagctc gagcccaagg tgcagaagat cttcgccgac cagtgcatct cgtgtcacgg
     2221 caccggcgac gacctcgtgc tctcgggctc gcccgagggg ctggtgcggg tccagtcgaa
     2281 gccgaccaag aagccgctga tcgtcgccgg agatccggac gccagctacc tgatcgccaa
     2341 ggtcaccggc gccaagggca tcgagggcga cccgatgccg ctcggcgaca ccatgcccaa
     2401 gcaggatgtc gaggtcctgc gccagtacat cgtcgcgctc ggcgaccgcg acaaggccgg
     2461 cgccggcggg gccgcgatga ccggcgaccc gagcagcggg cccaacctcc cggtcggcga
     2521 caccggcgtc gtcgcgggcg acggcccgac cgacagcgag cctgcgggtt cggacggtga
     2581 gccggccaag ccgagccgcc cgcccttcca cggcgtgttc cagatcaact tgccgtcgac
     2641 ctcgacgctc ggcaagcgca ccttcgagtt tcgcatcgac caccgcttcg gccgcgtcgg
     2701 cgcggagcgt ggcgcgttcg gcctcgacgc cggcgccaac atctcgtacg gcttcgccta
     2761 cggcatcctc gacggctggg acgtgttgct gcggcgcagt aactcgcgca agggctacga
     2821 gctcggcacc aagtacatcc cgatccgcca ggaggcgggc aagccgctga gcttcggtgg
     2881 ttacgcctcg atcgagtacc tgcgcgactt cccctcgaac accgccaacc cgtgggcggg
     2941 caacttccag ctgatgctca gccgcctgtg gttcgagcgc tggagcacca tgctgctcgt
     3001 cggctactcg ctgcgcaccg accacaaggc cgcgccgacc gtcgacttcg acggcaacgg
     3061 tggagcggcg ccggtgcgcg tccgcgacaa gcgcggcacg ctcggcgtcg gcctcgcctc
     3121 gaccgtgtgg atcggcgaga agaagcgctg gggcatcgac atggagtact ggctcccgat
     3181 cccctccaac aagttctact tcaacggtgg caacgccgac cccaacggca gcaacatcgg
     3241 ctcatggtcg ctgggcggct c
//
LOCUS       DASWNJ010001807         2394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_415086, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001807 DASWNJ010000000
VERSION     DASWNJ010001807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415086"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..514
                     /locus_tag="VGB85_30265"
     CDS             134..514
                     /locus_tag="VGB85_30265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0138423.1"
                     /translation="MTTGSHTHHGIDYVELAVPNIAAAKRFYEQAFGWSFTDYGPEYA
                     GFVDGPPASGRREVGGLREEPAGPRGSTAPLVLLFSTDLEASRERVRAAGGAISRDIF
                     AFPGGRRFHFTDPAGNELGVWAEP"
     gene            complement(528..>2394)
                     /locus_tag="VGB85_30270"
     CDS             complement(528..>2394)
                     /locus_tag="VGB85_30270"
                     /inference="COORDINATES: protein motif:HMM:NF023649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HEY0138424.1"
                     /translation="SYRLLVVSLACACNSEPPRVYDDPREPCADHNPRRNLYFGDLHV
                     HTAYSFDAWISDVRTDPAGAYRFARGEAVEGERLARPLDFAAVTDHAEYLAEVEGCTT
                     PGSAVYDSERCVGFRAADPKALVQFGLGLGSEDPQRFTDICGPGGIDCPARAAAVWQR
                     VQDAGEQAYDRSAACEFTSLIAYEWSGVKQLANLHRNVIFRTRDVPAAPISHFEAPTA
                     PELWTRLKGECREGLPGCDVLAIPHNSNWSNGNLFIPEAPAGSDPAALARLRSELEPL
                     LEIYQHKGDSECMNGISGLGEPDELCDFEKLRTGAVEDCGDGVGSNGIIARGCVSRLD
                     FLRGILLKGMQEEAKVGVNPYRLGVIASTDTHNGTPGKVEEASYSGHFGSLESDAVSR
                     LTAAVPAGPRNSGGGLVAVWAEENSREAIFDALRRREVYGTSGPRIAVRMFGGWDFPA
                     DLCERADLVDVADPRGVPMGGVLGERPEGGVPQIVVAAQRDVGTPEQPGTGLARVQII
                     KGWLDAGGDGHIAVFDVASAAEPGSVDEDTCEPRGVGADALCHLFKDPQYDPGLPTFY
                     YARILEDPTCRWSWRDCLALPVDERPAACSDPTLPRTIEERAWTSPIWVDPAGST"
BASE COUNT          374 a          867 c          776 g          377 t
ORIGIN      
        1 ctgcacgaga tgcacctgat cgtctcgcag tggaacaccg acaccgactg gccgtatcgc
       61 gcgatgcagt tcgtcaccgg cctcgatgaa tgaggcgccg cgcggtcatg cgccgtgata
      121 caaaccccgc gtcatgacca ccggatctca cacccatcac ggcatcgatt acgtcgagct
      181 cgcggtcccg aacatcgccg ccgccaagcg cttctacgag caggccttcg gctggagctt
      241 caccgactac ggcccggagt acgccggctt cgtcgacggt ccgcccgcca gcggccgccg
      301 tgaggtcggt ggattacgcg aggagccggc aggcccccgc gggtcgacgg cgcccctcgt
      361 gctgctgttc tcgaccgacc tcgaggccag ccgcgagcgg gtccgtgccg ccggtggtgc
      421 gatctcccgc gacatcttcg cgtttccggg tggacgccgg ttccacttca ccgaccccgc
      481 cggcaacgag ctcggcgtgt gggccgagcc gtgaacacgt ggccctctca ggtcgatcct
      541 gcagggtcca cccagatcgg gctggtccac gcgcgctcct cgatcgtgcg tggcagcgtc
      601 gggtcgctgc acgccgccgg ccgctcgtcc accggcagcg cgaggcaatc tcgccagctc
      661 caccggcacg tcggatcttc caaaatgcgc gcataataaa atgtcggcaa acccgggtcg
      721 tactgcggat ctttaaagag gtgacacagc gcgtcggcgc cgacacctct cggctcacag
      781 gtatcctcgt cgacgctacc cggctcggcc gcgctggcga cgtcgaacac cgcgatatgt
      841 ccatccccgc cagcatcgag ccagcctttg ataatttgca cacgcgcgag gccggtgccc
      901 ggctgctccg gcgtgccgac gtcgcgctgc gccgcgacca cgatctgcgg caccccgccc
      961 tccggtcgct cgccgagcac gccgcccatc ggcaccccgc gcgggtcggc gacatccacc
     1021 agatcggcgc gctcgcacag gtccgccgga aagtcccagc cgccgaacat gcgcacggcg
     1081 atccgcggac cgctggtgcc gtagacctcg cgccggcgca gcgcatcaaa aatcgcctcg
     1141 cggctgttct cctcggccca cactgccacg aggccaccac cgctgttgcg tgggcccgcc
     1201 gggaccgcgg cggtcaggcg agacacggcg tcgctctcga gcgacccgaa gtgcccgctg
     1261 tagctcgcct cctcgacctt gcccggcgta ccattgtgcg tatcagtgct ggcgatcacg
     1321 ccgaggcgat agggattgac cccgaccttc gcctcctcct gcataccttt taataaaatt
     1381 ccgcgcagga agtcgaggcg cgacacgcag ccgcgcgcga tgatcccgtt gctgccgaca
     1441 ccatcgccgc agtcctcgac cgcgccggtt cgtagctttt cgaaatcgca cagctcgtcg
     1501 ggctcgccga ggccgctgat cccgttcatg cactccgagt cgcccttgtg ttgatatatt
     1561 tccagaagcg gctcgagctc gctgcgcagc ctcgccagcg cggcggggtc ggagccggcg
     1621 ggcgcctcgg gtatgaacaa attcccgttg ctccaattgc tgttgtgcgg gatcgccagc
     1681 acgtcgcagc cgggcaggcc ctcgcggcac tcacctttta aacgcgtcca cagctccggg
     1741 gcggtcggcg cctcgaagtg ggagatcggc gcggcgggca cgtcgcgggt gcgaaagatg
     1801 acgtttcggt gcaagttggc gagctgcttc acgcccgacc actcgtaggc gatcaggctg
     1861 gtgaactcgc acgcggcgct acgatcgtag gcctgctcgc cggcatcctg cacgcgctgc
     1921 cacacggcgg ccgcgcgggc ggggcagtcg atcccgccgg gcccgcagat gtccgtgaag
     1981 cgctgcgggt cctcgctgcc gaggccgagc ccgaactgga ccagcgcctt cgggtccgcg
     2041 gcacgaaagc cgacgcagcg ctcgctgtcg tataccgccg agcccggggt ggtgcagccc
     2101 tcgacctccg cgagatattc ggcgtggtcg gtgaccgccg cgaagtcgag cggccgcgcg
     2161 aggcgctcgc cctcgaccgc ctcgccgcgc gcgaagcgat aggcgccggc cgggtcggtg
     2221 cgcacgtcgg agatccaggc gtcgaaggaa tacgcggtgt gcacgtgcag gtcgccgaag
     2281 tacaggtttc gccgcgggtt atgatcggcg cagggctcac gtggatcgtc gtagacccgc
     2341 ggcggctcgc tgttgcacgc acaggccagc gacacgacaa gcagacgata cgag
//
LOCUS       DASWNJ010001808         2992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_32095, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001808 DASWNJ010000000
VERSION     DASWNJ010001808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32095"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VGB85_30275"
     CDS             <1..786
                     /locus_tag="VGB85_30275"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0138425.1"
                     /translation="RGIVAQPRAAVSAHLGHAHAGSRAASLAKLREVLSDAKSFPARR
                     QAYERAQSRELAAHPLDLEALQPLFRRQAVLTVTAHRVSDILAALDLAREFDLRLAIV
                     GGADAWKVADELARAKVPVIVEPTLNLPGGFDSMGARLDNAALLHRAGAAVVLSAPGG
                     THNLRSLTQGAGIAVANGLPWEAALTAITLGPARAYGVDKTHGSVAVGKVANLVVWSG
                     DPFEFSSAPVQVYVRGALVRSTTRQTLLRDRYRDLEQFKQQSK"
     gene            856..1239
                     /locus_tag="VGB85_30280"
     CDS             856..1239
                     /locus_tag="VGB85_30280"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY0138426.1"
                     /translation="MPSHRPSTSQLEHGPIFSWYRDHNGFIFTSGHAAVDVETHSITL
                     GDLASETTATLSNLRRTLEKAGSGLDRVIKVTAYITDMSQFATFNSVYAKFFPPGSAV
                     PARTCVEVSRLPFNFKVEIEAVAHI"
     gene            1398..1898
                     /locus_tag="VGB85_30285"
     CDS             1398..1898
                     /locus_tag="VGB85_30285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138427.1"
                     /translation="MHDDDEQIVPGLRIGPFRLGEAEGPLRARLAGADVKEEERDTIR
                     VLEVGNVSLFVDGGVVTQVGVHGDHPARTSDGLRLGMTLREVPGRLVADLFDEVLLLD
                     GVAGLCFTTDEGLGDVDDDDEYSDEAVLELPGSDRITWIGVYTPLDEPLDEPLDEPAL
                     VAVALT"
     gene            complement(1980..>2992)
                     /locus_tag="VGB85_30290"
     CDS             complement(1980..>2992)
                     /locus_tag="VGB85_30290"
                     /inference="COORDINATES: protein motif:HMM:NF036491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138428.1"
                     /translation="YPSSANYTLDEYASQVVFEVTEACSEVNARQPTLLTESGRVVVA
                     NHAVTIADLREVQGELLPVPEATEDEHRLVAELRETLDNINSKNVDEYFHDAIDFRDE
                     CLQLFSRGYLSLQERACAEGLFHRIRTKVEKIVETLSHPSEEITDFLGKAQQKYLVNF
                     SIFQSIPDAWSIDQVFPAAPLSRHAEKPTLHAQIVDITCDSDGCVETFAHPDENLPYL
                     PLHERRGPDEKYYLGFFMTGAYQDSLANVHNLFGRCHEVILRNPDDDMVLPGSERVEL
                     SPSLTFEIKMGATCEDTLSAMDFDVERINKWLRDRHLAVETTLGESWAMGLLQSYPYL
                     SR"
BASE COUNT          455 a         1067 c         1000 g          470 t
ORIGIN      
        1 cgtggcatcg tcgcgcagcc gcgcgcggcc gtgtccgccc acctcgggca cgcccacgcc
       61 ggctcgcgtg ccgccagcct cgccaagctg cgcgaggtcc tcagcgacgc caagtccttc
      121 cccgcgcgcc ggcaggcgta cgaacgcgcc cagtcgcgcg agctggcggc gcaccccctc
      181 gacctcgagg cgctgcaacc actgtttcgt cgccaggcgg tcctcaccgt cactgcgcac
      241 cgcgtctccg acatcctcgc tgccctcgac ctcgcccgcg agttcgacct gcgtctggcg
      301 atcgtcggcg gggccgatgc gtggaaggtc gccgacgagc tcgcgcgggc caaggtcccc
      361 gtcatcgtcg agccgaccct caacctcccc ggcggcttcg acagcatggg cgcccgcctc
      421 gacaacgccg cgctgctgca ccgcgccggt gcagccgtcg tcctgtcggc gcccggcggc
      481 acccacaacc tgcgcagcct gacgcagggc gccggcatcg ccgtcgccaa cggtctcccg
      541 tgggaggccg ccctcacggc gatcaccctc ggacccgcgc gcgcctatgg cgtcgacaag
      601 acccacggca gcgtcgccgt cggcaaggtc gccaacctcg tcgtctggag cggcgacccc
      661 ttcgaattct cgagcgcccc ggtgcaggtc tatgtgcgcg gcgcgctggt ccgcagcacc
      721 acccggcaga ccctcctgcg cgaccgctac cgcgacctcg aacaatttaa acagcagagc
      781 aaataaattc tgcgcctacg tggttctcct gcttcgtgac gcccgcgacc ggcccggggt
      841 atagatcgat cgcgtatgcc gagtcaccgc cccagcacct cccagctcga gcacgggccc
      901 atcttctcgt ggtaccgcga ccacaacggc ttcatcttca cctcggggca cgcggcggtc
      961 gacgtcgaga cccacagcat caccctcggt gacctcgcca gcgagacgac cgcgacgctg
     1021 agcaacctcc ggcgcacgct cgagaaggcc ggctcgggcc tcgaccgcgt gatcaaggtg
     1081 acggcctaca tcacggacat gagccagttc gcgaccttca actcggtgta cgcgaagttc
     1141 ttcccgcccg gctccgccgt cccggcccgc acctgcgtcg aggtctcgcg cctgcccttc
     1201 aatttcaagg tcgagatcga ggcggtcgcc cacatctgaa gcccgcgcga agtgtgcggc
     1261 ggtcccaccg gcgggcgtgt cgccgtgcgc gtgcccctgg tgggcaatca cagcaggacg
     1321 gcgtgtagac gggccctgcc gtcatcctgc caccgccgga cttccgcccg cgctcgtcga
     1381 gcgctaggct tcgcgcgatg cacgacgacg acgaacagat cgttccaggc ctgcggatcg
     1441 gcccgtttcg cctcggtgag gccgaaggcc cgctgcgcgc ccgcctcgcc ggcgccgacg
     1501 tgaaagagga ggagcgcgac accatccgcg tgctcgaggt cggcaacgtc agcctgttcg
     1561 tcgatggcgg cgtcgtgacg caggtcggcg tgcacggcga tcaccccgca cgcaccagcg
     1621 acggcctgcg gctcggcatg accctgcgcg aggtccctgg caggctggtc gccgacctgt
     1681 tcgacgaggt gctgctgctg gacggcgtcg cgggcctgtg cttcaccacg gacgagggcc
     1741 tcggcgacgt cgacgacgac gacgagtaca gcgacgaggc cgtgctcgag ctgcccggca
     1801 gcgaccggat cacgtggatc ggcgtctaca cgccgctcga cgagccgctc gacgagccgc
     1861 tcgacgagcc cgcgctcgtc gccgtcgccc tgacgtgatg cggcgccgcg tcgtcggcga
     1921 gagatgcgcc tccaccgggc agaccgcggt ctcgcaagac cgcggcctca tgtgaagcct
     1981 cagcgcgaca ggtacgggta gctctgcagg agccccatcg cccacgactc gccgagcgtc
     2041 gtctcgaccg cgaggtgccg gtcgcgcagc catttattga tacgctcgac atcaaaatcc
     2101 atcgccgaga gcgtgtcctc gcaggtcgcg cccatcttga tctcgaaggt caggctcggt
     2161 gacagctcga cgcgctcgga gcccgggagg accatgtcgt cgtcggggtt acgaagaatg
     2221 acctcgtggc agcggccgaa caggttgtgg acgttcgcga ggctgtcctg atacgcgccg
     2281 gtcatgaaga agccgaggta gtacttctcg tccggcccgc gccgctcgtg caacggcagg
     2341 tagggcaggt tctcgtcggg gtgggcgaag gtctcgacgc agccgtcgct gtcgcaggtg
     2401 atgtcgacga tctgcgcgtg cagggtcggc ttctcggcgt ggcgtgacag cggggccgcc
     2461 gggaacacct gatcgatgct ccacgcgtcg gggatcgact ggaagatcga gaaattcacg
     2521 aggtacttct gctgcgcttt gccgaggaag tcggtgatct cctcggaggg gtgcgacagg
     2581 gtctcgacga tcttctcgac cttggtgcgg atgcggtgga acaggccctc ggcgcaggcg
     2641 cgctcttgca gcgacaggta gccgcgcgag aacagctgca agcactcgtc gcggaagtcg
     2701 atcgcatcgt gaaagtactc gtcgacgttc ttcgagttga tgttgtcgag ggtctcgcgc
     2761 agctcggcga cgaggcggtg ctcgtcttcg gtggcctcgg gcacgggcag cagctcgccc
     2821 tgcacctcgc gcaggtcggc gatcgtgacg gcgtggttgg cgacgacgac gcggccgctc
     2881 tcggtgagca gggtcggctg gcgggcgttg acctccgagc aggcctcggt gacctcgaac
     2941 accacctgcg aggcgtactc gtcgagggtg tagttggccg acgaggggta cg
//
LOCUS       DASWNJ010001809         1952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_628337, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001809 DASWNJ010000000
VERSION     DASWNJ010001809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628337"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VGB85_30295"
     CDS             <1..877
                     /locus_tag="VGB85_30295"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY0138429.1"
                     /translation="GDTTTGDTTGDTTDASTGTTDGTTGEDAWTTELCVEICVALTGT
                     DMWSVDACHKLGVDAEGNIEIKCVEIVEHCDGRSHICIGSRGVTAGADPVAAYFARAA
                     HDEAASVYAFAALEQELAALGAPDDLLARVRAAAADEVRHAQAIARLAADRGGRCKPP
                     ERRAFAVRGALEIAIENAIEGCVRETWAALLAAHQAEHAQDPEIRAVMRTIAADEARH
                     AELAWAIDRWLQGTLDLGGQARVASARAAAAASIVAGVAASEPATACVAVAGVPDRAS
                     ATRLCRGLAAALWAA"
     gene            954..1415
                     /locus_tag="VGB85_30300"
     CDS             954..1415
                     /locus_tag="VGB85_30300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138430.1"
                     /translation="MEASKRSPASVMEHVFTHRNPTTGSTPPEHMAWVVFEHGTAFFS
                     APSDELPLDATPEEILAAARIAMAELGPVIAGTAAGDFNPVCLDAWFPGEFVYFVTYD
                     HPAIATVVIADHEDDLSAGLAGRAYREADLAGLTATEIRGFDGRVVQPRPD"
     gene            1443..>1952
                     /locus_tag="VGB85_30305"
     CDS             1443..>1952
                     /locus_tag="VGB85_30305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138431.1"
                     /translation="MTSPRSGMLVLAGLLATPMSVRAAEGDLLLEMHFKPVPRTQLAI
                     WLEDAEGKWVQDVLVTQATGKLGIGNRPGAWNFVSSWRAPYGPRPSVLPVWAHRRGKT
                     YPRLVFFDSDAADAQSLGWHENSSSPETYFCRPLTASEDATISVDSMTCPSPSVFETD
                     KGAFHPSDKS"
BASE COUNT          262 a          725 c          703 g          262 t
ORIGIN      
        1 cggcgacacc accaccggcg acaccaccgg cgacaccacc gacgcgagca ccggcaccac
       61 cgatggtacg accggcgagg atgcctggac gacggagctg tgcgtcgaga tctgcgtcgc
      121 gctgaccggc acggacatgt ggtcggtcga cgcttgccac aagctcgggg tcgacgccga
      181 gggcaacatc gagatcaagt gcgtcgagat cgtcgagcac tgcgacgggc gcagtcacat
      241 ctgcatcggt tcgcgtggtg tcaccgcggg ggccgacccg gtcgccgcct acttcgcccg
      301 cgccgcgcac gacgaggccg cctcggtgta cgccttcgcg gcgctcgagc aggagctcgc
      361 ggccctcggc gcccccgacg acctgctggc ccgcgtgcgc gccgcggcgg ccgacgaggt
      421 ccgccacgcg caggcgatcg cccgcctcgc ggccgaccgt ggcggccgct gcaagccgcc
      481 cgagcggcgc gccttcgcgg tccggggcgc cctcgagatc gcgatcgaga acgcgatcga
      541 gggctgcgta cgcgagacct gggccgccct gctcgccgcg catcaggccg agcacgcgca
      601 ggacccggag atccgcgccg tcatgcggac gatcgccgcc gacgaggcgc ggcacgccga
      661 gctcgcgtgg gcgatcgacc gctggctgca gggcacgctc gatctcggcg gccaggcccg
      721 ggtcgccagc gcgcgcgcgg ccgcggccgc ctcgatcgtc gcgggcgtgg ccgccagcga
      781 gcccgccacc gcctgcgtcg cggtcgccgg cgtgcccgac cgcgcgagcg cgacgcggct
      841 gtgccggggc ctggccgccg cgctgtgggc cgcctgagcg gtcggcctcg cgcgcgaccg
      901 tgcgcggggt cagcgcttgg catccttcgg gccggcgggc atgctgcggg gtcatggaag
      961 cgagcaagcg gagcccagcc tcggtgatgg agcacgtgtt cactcatcgc aacccgacga
     1021 cgggctcgac gccgccggag cacatggcgt gggtggtgtt cgagcacggc actgctttct
     1081 tctccgcgcc gtccgacgag ctcccgctcg acgcgacgcc ggaggagatc ctcgccgccg
     1141 cgaggatcgc catggccgag ctcgggccgg tgatcgcggg caccgcggcc ggcgacttca
     1201 acccggtgtg cctcgatgcc tggttccctg gtgagttcgt gtacttcgtg acctatgatc
     1261 atcctgcgat cgcgaccgtc gtgatcgccg atcacgagga cgacctgtcg gcggggctcg
     1321 cgggccgggc ctatcgtgag gccgacctcg ccgggctcac cgccaccgag atccgcggct
     1381 tcgacggacg cgtggtccag ccgcgccccg actgagcccg cgaggccgtg tatactccgc
     1441 ccgtgacctc gcctcgcagc gggatgctcg tgctcgcagg gttgctcgcg acccccatgt
     1501 ccgtccgcgc ggccgagggc gacctgctgc tcgagatgca cttcaagccg gtgccacgga
     1561 cccagctggc gatctggctc gaggacgccg agggcaagtg ggtgcaggac gtgctcgtca
     1621 cccaggcgac cggcaagctc gggatcggca accggcccgg cgcgtggaac ttcgtgtcga
     1681 gctggcgcgc gccgtacggg ccgcggccct cggtgctgcc ggtgtgggcg catcgccgcg
     1741 gcaagaccta tccgcggctg gtgttcttcg acagcgacgc cgccgacgcg cagtcgctcg
     1801 gctggcacga gaacagcagc agccccgaga cctacttctg tcgcccgctg accgcctccg
     1861 aggacgcgac gatctccgtc gactcgatga cctgcccgtc gccctcggtg ttcgagaccg
     1921 acaagggcgc gttccatccg agcgacaaga gc
//
LOCUS       DASWNJ010001810         1668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_491797, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001810 DASWNJ010000000
VERSION     DASWNJ010001810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491797"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..625)
                     /locus_tag="VGB85_30310"
     CDS             complement(158..625)
                     /locus_tag="VGB85_30310"
                     /inference="COORDINATES: protein motif:HMM:NF013853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 domain-containing protein"
                     /protein_id="HEY0138432.1"
                     /translation="MTLYETDFVLWTEDQAGKLRQARDSGSDLDLDWQHLIEEVEWLG
                     ITDRRALLTDVMGVLEHLAKLEWSPAFDSRRAWADRIDGHRDRIATLLRQSPCLREVL
                     HGAINDGWELTRPRVERDLERSRAGLELPVGCPYAAEQVLGSEWFPHNRYGFE"
     gene            922..>1668
                     /locus_tag="VGB85_30315"
     CDS             922..>1668
                     /locus_tag="VGB85_30315"
                     /inference="COORDINATES: protein motif:HMM:NF016090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEY0138433.1"
                     /translation="MGASATGAGAFNGYDPGPYVCEMTRAAEAESPLGRLILERFAAV
                     PLPELRARARDAEGELFSQGITFTVYSDKDAIDRILPFDVIPRVIGADDWDVIERGIC
                     QRVRALNLFLADVYGPQRSLAEGVIPADLVLGNSNYRPEMRGVALPHGTYVHIAGTDL
                     VRDGEGRFRVLEDNARTPSGVSYVVENRHLMLRSFPDMMRDLPVRPVSDYGRRLHEVL
                     SEVAPPAILDPQVVLLSPGVFNSAYFEHVFL"
BASE COUNT          225 a          607 c          577 g          259 t
ORIGIN      
        1 gtatcctgcg ttcgagatcc tcgttcacgc tgccgtccta tcctgctggt gtggtgcctc
       61 gcctaaccct ggtcaacgct gacaacagcg ctgcgcggta gaggtttcgt cggacctcgc
      121 gcctgtagca tcatcgcccg agccgtccgg gccggagcta ctcgaagccg tagcggttgt
      181 gcgggaacca ctccgacccg agcacctgct cggccgcgta ggggcagccg acgggcagct
      241 cgagcccggc gcggctgcgc tccaggtcgc gctcgacccg gggcctggtc agctcccagc
      301 cgtcgttgat cgcgccgtgg agcacctcgc gcaggcaggg gctctgccgg agcagggtcg
      361 cgatccggtc gcggtgcccg tcgatccggt cggcccaggc gcggcgggag tcgaaggccg
      421 gcgaccactc cagcttcgcc aggtgctcga gcacgcccat cacgtcggtc aggagcgcgc
      481 ggcggtcggt gatgccgagc cactccacct cctcgatcag gtgctgccag tccaggtcga
      541 ggtcgctgcc ggagtcgcgc gcctggcgca gctttcccgc ctggtcctcg gtccaaagca
      601 cgaagtcggt ctcgtagagc gtcacgccca tgcactcgca cccgcccgac gtccgcgcga
      661 cgaagcggcg gtcctgcgtc cacctcgggg tcggccacca agctaaccgc gacccgggga
      721 ggcgccaacc cccgggtcgc ccggcgcctc gccgaccagg gcgttgccgc ctggaggtgc
      781 agccggtggg cgcgatttgg gcaacaggcc cgccgcggcg atcgctgccg gtctcggccg
      841 ccgatcgtgc ttggctaatg gcatagggtt tgcattatta ggccgatgac aacgaataat
      901 cgggctgcgg cgggagctgc catgggagcg agcgcgacgg gcgcgggcgc gttcaacggt
      961 tacgatcccg gcccctacgt ctgcgagatg acgcgcgccg cggaagccga gagcccgctc
     1021 gggcggctga tcctggagcg gttcgcggcc gtgccgctgc cggagctgcg ggcgcgcgcc
     1081 cgcgacgccg agggcgagct gttcagccag gggatcacct tcaccgtcta cagcgacaag
     1141 gacgcgatcg accgcatcct gccgttcgac gtgatccccc gcgtgatcgg cgccgacgac
     1201 tgggacgtga tcgagcgtgg catctgccag cgggtgcggg ccctcaacct gttcctcgcc
     1261 gacgtctacg ggccgcagcg ctcgctggcc gagggcgtga tcccggccga tctcgtcctc
     1321 gggaactcga actaccggcc cgagatgcgc ggggtcgccc tgccgcacgg cacctacgtg
     1381 cacatcgccg ggaccgacct cgtgcgcgac ggcgagggcc ggttccgggt cctggaggac
     1441 aacgcccgca cgccctcggg cgtctcctac gtggtcgaga accggcacct gatgctgcgc
     1501 tcgttcccgg acatgatgcg cgacctgccc gtccggcccg tctcggacta cggccgccgg
     1561 ctgcacgagg tcctctcgga ggtggccccg ccggcgatcc tcgaccccca ggtcgtgctg
     1621 ctctcgccgg gcgtcttcaa cagcgcctac ttcgagcacg tcttcctg
//
LOCUS       DASWNJ010001811         5898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_628395, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001811 DASWNJ010000000
VERSION     DASWNJ010001811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5898
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628395"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="VGB85_30320"
     CDS             <1..1164
                     /locus_tag="VGB85_30320"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEY0138434.1"
                     /translation="TYARGADESFVDGVQYLLTRLLMGPDFMYRVELGVPVPGDSEVV
                     RLTSWELASRLSYLLWQGPPDDVLLDAAEADALATPEQLAMQVDRMLADPRARDTVQD
                     YFRQLLELDELAQFSKAGAPASIGPLLRAETERFIDHVVWESEGTLRELLSASYTIIP
                     KELAYLYNVILTEFPADAVPGEAYLVQLDPTQRAGLLTHGSLMSILATPSASRPIARG
                     KFVREKLLCAAVPPPPPGVNATPPPPDPSATTREQYEQHAEDPACNGCHSTFDGLGFA
                     FEHYSAIGQYRLQENGKPIDASGSVPDIDIGGPFTDGVELALRLADHELTLRCTTKQM
                     LRFGYGRVEGLKDACTIDDLAAQFTAADGDIKSLLRALTTTEAFLYRRAGGSP"
     gene            1161..2567
                     /locus_tag="VGB85_30325"
     CDS             1161..2567
                     /locus_tag="VGB85_30325"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEY0138435.1"
                     /translation="MSKPSRTFISRRTVLRGAGRVAVCLPLLEAMACDPALRAQGKSG
                     GVTPRAGGAPKRLIVFFVPNGTIPPEWFPDPSASETDFTLKSLMAPLAPYREQMLILS
                     GVDNQVYLKGIVDNHNEGMTTLLTGMPAGDLLLPPEQRIIQQANGISLDQYVADAIGG
                     GTVMPSLPIGYEWYHWLYDKNRNGIQHQGSPSQLFTALFGDPNADPAALDKLVARRKS
                     ILDDVATDYDNLLPKVSGADRLKLEEHLEAIRALEMGIGNTTSCKPEMPMYPAEYPQP
                     QVDPAGLPLWVEHMTDLLALSLACDATRVSTFFIRHGGGGQSFFPWLGMDIIPEEPNL
                     PTTEHHAMSHSWSAQPYPYDNLVKIHTWFSEQIAMLCGKLQAHDELGGSVLDNSVILV
                     CSENAEGSHTKVNMPFLLIGSAGGHFRTGRHVQCGGAPHNRLLLSLAEAFGLPGPDGS
                     PDHFGDPDFGGGPLSGLT"
     gene            2767..>5898
                     /locus_tag="VGB85_30330"
     CDS             2767..>5898
                     /locus_tag="VGB85_30330"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0138436.1"
                     /translation="MSAPETDPDEHRSRSELLAELRALRAQVADGVLLQQMRLIFAVA
                     PVLMTYVDADGCYRLVNQQYADWFGRTPEELPGRHMREILGEAAYARARPHVEAALDG
                     RLTRFDIELMHRDGSMRWVSVTYAPHQAPDGEVLGFVGMAMDMTARKRQERELRASRE
                     QLEVVLRGVTDAIMMQDHHGRIVYANPVAAATLGIPSVEALLALTSDELHDRFVMLDS
                     DGNLVPPERAPVRRAARGEHVQDHLVRFRTHDSDADRWSAVSSTPVCDELGAVRYVVN
                     IFRDITERRDAERALQAQHQLTRTITDNATSALFLMDTRQHCTFMNPAAEAMLGYTLD
                     EVQGRPLHELIHHHHPDGRPYPISECPIDRALPQRQHERGEDVFIRKDGSFFPVAFTA
                     SPIVTDGQPVGTVIEVRDITADLLVQDELRLRARVLQSMSEGVSVADERGYIIFTNAA
                     EDAMFGYAAGELVGQHVSVQNSYPPEENRRIVASVIEHLKLHGAWVGEFHNKKKDGTP
                     FITHSRITTLELSGKRHLLCVQEDITQEKRDREALRFLADASTALVASLDPAETLQSL
                     TRLAVPRLGSWCTVYLHESGGAPHEVVELAALTHIDPDKEAVIRTLHARFPADLVHGQ
                     GLGKVLRTGAPQLYAQIDDGMLAQAARDPAELELLLQLAPRSWMMAPLRVQGRTFGAI
                     SIVSSTARRYDPGDLALLEEIARRASVAVDNARLFDMAQKERARAEEANRAKDLFLST
                     LSHELRTPLTAILGWTRMLRSHTLSEEKRAKGLETIDRNARAQVALIEDILDLSRIVT
                     GKLRLDLESVELTAVVEAAVETVRPAADARNIRLLLALDPDAGPVIGDPNRLQQIVWN
                     LLTNAVKFTPRGGQVTVSLRREATHHQMDGMVELVVADDGQGIAPMFLPHVFDRFRQG
                     DASTTRSQGGLGLGLAIVKHLTELHGGTIEAASDGEGLGAAFTVRLPVRTPAPAAEVP
                     AGRDGPELECPPELDGLRVLVVDDEDDAREWIASLLEHCKAVVLTASSAAQALEVLRT
                     ERPNIMVSDIGMPG"
BASE COUNT          911 a         2279 c         1891 g          817 t
ORIGIN      
        1 acctacgccc gcggcgccga cgagagcttc gtcgacggcg tgcagtacct gctgacgcgc
       61 ctgctgatgg gccccgactt catgtaccgc gtcgagctcg gcgtacctgt ccccggggac
      121 agcgaggtcg tgcgcctgac ctcgtgggag ctcgcctcgc gcctctctta tttgctctgg
      181 caaggcccgc cggacgacgt cctgctcgac gccgccgagg ccgacgccct cgccacgccg
      241 gagcagctcg ccatgcaggt cgaccgcatg ctcgccgacc cgcgcgcgcg cgacaccgtg
      301 caggactact tccgccagct gctcgagctc gatgagctcg cgcagttcag caaggccggc
      361 gcgccggcct cgatcggccc gctgctgcgc gccgagaccg agcgcttcat cgaccacgtc
      421 gtctgggagt ccgagggcac cctgcgcgag ctgctgtcgg ccagctacac gatcatcccg
      481 aaggagctgg cgtacctcta caacgtgatc ttgaccgagt tcccggccga cgccgtgccc
      541 ggcgaggcct acctcgtgca gctcgacccg acccagcgcg ccggcctgtt gacgcacggc
      601 agcctgatgt cgatcctcgc cactccgtcg gccagccgcc cgatcgcccg cggcaagttc
      661 gtgcgcgaga agctgctgtg cgccgccgta ccgccgccgc cgccgggcgt caacgccacg
      721 ccgccgccgc ccgacccgag cgccaccacg cgcgagcagt acgagcagca cgccgaggac
      781 ccggcctgca acggctgtca cagcaccttc gacggcctcg gcttcgcctt cgagcactac
      841 agcgcgatcg gccagtatcg cctgcaggag aacggcaagc cgatcgacgc ctccggcagc
      901 gtgcccgaca tcgacatcgg cggccccttc accgacggcg tcgagctcgc gctgcgcctc
      961 gccgaccacg agctgaccct gcgctgcacc accaagcaga tgctgcgctt cggctacggc
     1021 cgggtcgagg gcctcaagga tgcctgcacg atcgacgacc tcgccgcaca gttcaccgcc
     1081 gccgacggcg acatcaagtc gctgctgcgc gccctgacca ccaccgaagc cttcctctac
     1141 cgccgcgccg ggggttcgcc gtgagcaagc cgagccgcac cttcatcagt cgtcgaaccg
     1201 tcctgcgcgg ggctggccgc gtcgccgtgt gcctgccgtt gctggaggcg atggcctgcg
     1261 acccggccct gcgcgcgcag ggcaagtccg gcggcgtcac gccacgcgca ggcggggccc
     1321 cgaagcgcct gatcgtgttc ttcgtcccga acggcacgat cccgccggag tggttccccg
     1381 acccctccgc ctcggagacc gacttcacgc tcaagtcgct gatggccccg ctcgcgccct
     1441 accgcgagca gatgctgatc ctcagtggcg tcgacaatca ggtctacctc aagggcatcg
     1501 tcgacaacca caacgagggc atgacgaccc tgctgaccgg catgccggcc ggcgacctgc
     1561 tgctcccgcc cgagcaacgg attatccaac aggccaacgg catctcgctc gatcaatacg
     1621 tcgccgacgc gatcggcggc ggcaccgtca tgccctcgct gccgatcggc tacgagtggt
     1681 accactggct gtacgacaag aaccgcaacg gcatccagca tcagggcagc ccttcgcaat
     1741 tgtttacggc gctgttcggc gatcccaacg ccgaccccgc cgcgctcgac aagctggtcg
     1801 cgcggcgcaa gagcatcctc gacgacgtcg cgactgacta cgataatttg ctgccaaaag
     1861 tcagcggcgc cgaccgcctc aagctcgagg agcacctcga ggcgatccgc gcgctcgaga
     1921 tgggcatcgg caacaccacc tcgtgcaagc ccgagatgcc gatgtatccg gccgagtacc
     1981 cgcagccgca ggtcgacccc gccggcctgc cgctgtgggt cgagcacatg accgacctgc
     2041 tcgccctctc gctcgcctgc gacgcgaccc gcgtctcgac ctttttcatt cgtcacggcg
     2101 gcggcggcca gtcgttcttc ccgtggctcg gcatggacat catccccgag gagccgaacc
     2161 tgccgaccac cgagcaccac gcgatgtcac actcgtggtc ggcccagccg tatccatatg
     2221 acaacctcgt caagatccac acctggttct ccgagcagat cgcgatgctg tgcggcaagc
     2281 tccaggccca cgacgagctc ggcggcagcg tgctcgacaa ttcggtcatc ctcgtgtgct
     2341 cggagaacgc cgagggcagc cacaccaagg tcaacatgcc gtttttgctg atcggcagcg
     2401 ccggcggcca cttccggacc ggacgccacg tgcaatgcgg cggcgccccg cacaaccgcc
     2461 tgctgctctc gctcgcggag gccttcggcc tgccgggccc cgacggctcg cccgaccact
     2521 tcggcgaccc cgacttcggc ggcggcccgc tctccggcct gacctgagcg cctcgccggt
     2581 cgcgcgggct gcacggtcat cgctgccggc cgcgtggtcg tgcgggcgcg gggctcacgc
     2641 cgctggcacg gatggcctgt acgtggccgt ccgcgggccc gggctgacgg cgccggggat
     2701 cccccatggc ggcatcgggg acctgcggtt atgttctgcg gcccggagcg gccaaccagg
     2761 caaccgatgt cagctcccga aacagacccg gacgagcaca ggtcgcgctc ggagctgctc
     2821 gccgagctgc gggccctgcg agcccaggtc gccgacggcg tcctgctgca gcagatgcgc
     2881 ctgatcttcg ccgtcgcgcc ggtgctgatg acctacgtcg acgccgacgg ctgctatcgc
     2941 ctcgtcaacc agcaatacgc ggactggttc ggccgcacgc cggaggagct gcccgggcga
     3001 cacatgcggg agatcctcgg cgaggccgcg tatgcgcgcg cgcggccgca cgtcgaggcc
     3061 gcgctcgacg gccgactgac gcgcttcgac atcgagctga tgcaccgcga cggcagcatg
     3121 cgctgggtga gcgtcaccta cgcgccgcac caggcccccg acggcgaggt gctgggcttc
     3181 gtcggcatgg cgatggacat gaccgcccgc aaacgccagg agcgcgagct gcgggcctca
     3241 cgggagcagc tggaggtcgt gctgcgcggc gtgacggacg cgatcatgat gcaggaccac
     3301 cacggccgga tcgtctacgc caacccggtc gccgcggcga cgctcggcat ccccagcgtc
     3361 gaggcgctgc tcgccctcac cagcgacgag ctgcacgacc gcttcgtgat gctcgacagc
     3421 gacggcaacc tcgtcccccc cgagcgcgcc ccggtccgca gggccgctcg cggcgagcac
     3481 gtccaggacc acctcgtccg cttccggacc cacgacagcg acgccgaccg ctggtcggcc
     3541 gtgtcgtcga ccccggtgtg cgacgagctc ggcgcggtcc gctacgtcgt caacatcttc
     3601 cgcgacatca ccgagcgccg cgacgccgag cgggcgctgc aggcccagca ccagctgacc
     3661 cgcacgatca ccgacaacgc cacctcggcg ctgttcctga tggacacgcg gcagcactgc
     3721 accttcatga acccggccgc ggaggcgatg ctcgggtaca cgctcgacga ggtgcagggc
     3781 cggccgctgc acgagctcat ccaccaccac caccccgacg gccggcccta cccgatcagc
     3841 gagtgcccga tcgaccgcgc cctgccgcag cgccagcacg agcgcggcga ggacgtgttc
     3901 atccgcaagg acggcagctt cttcccggtc gccttcaccg ccagcccgat cgtcaccgat
     3961 ggtcagccgg tcggcaccgt catcgaggtc cgggacatca ccgccgacct gctcgtccag
     4021 gacgagctgc gcctgcgcgc ccgcgtgctg cagagcatgt ccgagggcgt cagcgtcgcc
     4081 gacgagcgcg gctacatcat cttcaccaac gccgccgagg acgcgatgtt cggctacgcc
     4141 gccggtgagc tcgtcggcca gcacgtctcc gtccagaaca gctacccgcc ggaggagaac
     4201 cgccgcatcg tcgcctccgt catcgagcac ctcaagctcc acggcgcgtg ggtcggcgag
     4261 tttcacaaca agaaaaaaga cggcaccccc tttatcaccc actcgcgcat caccaccctc
     4321 gagctgtccg gcaagcgcca tttgttgtgc gtgcaggagg acatcacgca ggagaagcgc
     4381 gaccgcgagg cgctgcgctt cctcgccgac gccagcaccg cgctcgtcgc ctcgctcgac
     4441 ccggcggaga cgctgcaaag cctgacgcgc ctggcggtgc cgcggctcgg ctcgtggtgc
     4501 accgtgtacc tgcacgagtc tggcggcgcc cctcacgagg tggtcgagct cgccgcgctg
     4561 acgcacatcg accccgacaa ggaggcggtg atccgcacgc tgcacgcccg cttcccggcc
     4621 gacctcgtgc atggtcaggg gctcggcaag gtgctgcgga ccggcgcgcc gcagctgtac
     4681 gcccagatcg acgacggcat gctcgcgcag gcggcccgcg acccggccga gctcgagctg
     4741 ctgctccagc tcgcgccgcg ctcgtggatg atggcgccgc tgcgggtcca gggccgcacc
     4801 ttcggggcga tctcgatcgt cagctcgacc gcccgccgct acgaccccgg tgacctcgcg
     4861 ctgctcgagg agatcgcgcg ccgggcctcg gtcgccgtcg acaacgcccg cctgttcgac
     4921 atggcccaga aggagcgcgc gcgggccgag gaggccaacc gcgccaagga cctgttcctg
     4981 agcaccttga gccacgagct gcgcaccccc ctgacggcga tcctcggctg gacccgcatg
     5041 ctgcgcagcc acacgctgtc ggaggagaag cgcgccaagg gcctcgagac gatcgaccgc
     5101 aacgcccgcg cgcaggtcgc cctgatcgag gacatcctcg acctcagccg gatcgtcact
     5161 ggcaagctgc gcctcgacct cgagtccgtc gagctgaccg ccgtcgtcga ggccgccgtc
     5221 gagacggtgc ggcccgccgc cgacgcccgc aacatccgcc tgctgctcgc cctcgacccc
     5281 gacgccggcc cggtgatcgg cgaccccaat cgcctgcagc agatcgtctg gaacctgctc
     5341 accaacgccg tcaaatttac gccgcgcggc ggccaggtga cggtgtcgct gcgccgcgag
     5401 gcgacgcatc atcagatgga cgggatggtc gagctcgtcg tcgccgacga cggccagggc
     5461 atcgcgccga tgttcctgcc gcacgtcttc gaccgcttcc gtcagggcga cgcgagcacg
     5521 acccgcagcc agggcggcct cggcctcggc ctggcgatcg tcaagcacct gacggagctg
     5581 cacggtggca cgatcgaggc cgccagcgac ggcgagggcc tcggcgccgc cttcacggtg
     5641 cggctccccg tgcgcacacc ggcgcccgcc gccgaggtcc ccgcggggcg cgacggcccc
     5701 gagctcgagt gcccgcccga gctcgacggc ctccgggtgc tcgtcgtcga cgacgaggac
     5761 gacgcccgcg agtggatcgc ctcgctgctc gagcactgca aggccgtggt gctgaccgcg
     5821 tcctcggccg cgcaggcgct cgaggtgctc cgcaccgagc gccccaacat catggtctcc
     5881 gacatcggca tgcccggc
//
LOCUS       DASWNJ010001812         4175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_32157, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001812 DASWNJ010000000
VERSION     DASWNJ010001812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32157"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VGB85_30335"
     CDS             <1..237
                     /locus_tag="VGB85_30335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138437.1"
                     /translation="LVAFATKWLSMPFLWFGTGSIVDPGAPMLQPTDMLVTRERVVEP
                     VVLGRRGALALTAFGAALYVAAVVMLLIGSPPKL"
     gene            357..719
                     /locus_tag="VGB85_30340"
     CDS             357..719
                     /locus_tag="VGB85_30340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138438.1"
                     /translation="MSNYAILIDSEHAKVFSFDAGEVTNHAYHRHEPQHHTGHTQDQE
                     KKSEHFFHQVAEHLKGAEHLLLMGSGLGKEHFKTHLESHHHELAKLIVGVDTVDHPTD
                     NQLVAHAREAFSKHNVKL"
     gene            complement(725..2047)
                     /locus_tag="VGB85_30345"
     CDS             complement(725..2047)
                     /locus_tag="VGB85_30345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0138439.1"
                     /translation="MLLLALAGGAPALLCALALLVTEAHSVKVRWTFGALATGMWLGC
                     ALIVYVRVVRSLHVVSGLLAALREEDFSMRGRITGHGPADDALSGVLREVNGLADILR
                     EQRLGAFEAGALLRTVMEEIDVAVLAFDAGGKLRLANRAAERLLDADTGVLGRDAASL
                     GLAACLEGEAPRTIGTVFPGGPGPWELRRTQFRQRGLPHTLLVLTDLRRALREEERQA
                     WQRLVRVLGHEINNSLAPIHSIAANLREQLDGVTEVRPPDLDEDLARGLDVITRRSEA
                     LARFLSAYAKLARLPPPRPGAVEVATWVDRVVALERRVPVHVRAGPAAIVQADGDQLD
                     QLLINLVRNAADATLAARSPAAVVVSWSAGGGAIELVVRDDGPGLAETANLFVPFFTT
                     KPDGSGIGLVLSRQIAEAHGGALSLANREDGERGCEARVRLPDARLGP"
     gene            complement(2092..3480)
                     /locus_tag="VGB85_30350"
     CDS             complement(2092..3480)
                     /locus_tag="VGB85_30350"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0138440.1"
                     /translation="MSEATTRAVSPVRVLIADDQADVLEALRLLLKAQGFAPQLARSP
                     AEILAAIEAQEFEALLMDLNYTRDTTSGREGLDLIARVTALDAHLPVLVMTAWGSVDG
                     AVEAIRRGARDYIQKPWDNTRLLATLRTQVELARALRRSGKLERENERLRAGEGLPEL
                     VAGSRAMEPVLRIMQRVAPSDAGVLITGEHGTGKEVVARWLHAASLRASRPFVAVNAG
                     ALGEGVFESELFGHVKGAFTDARAERIGCFELADGGTLFLDEIANMPPGQQAKLLRVL
                     QTGELQRVGSSKLRRVDVRVLAATNAPLSKLTGDGTFREDLLYRLNTVEIHLPPLRER
                     REDILRLAQHFLRARSQRYGGKVVDLDAAAKQALLEHRWPGNVRELEHVIERALLLAS
                     GPEIRVEDLVLRPGSARAPAFDEMTLDEAERYLIVLALARANNNVSEAAQKLGLSRSA
                     FYRRLVRHGLET"
     gene            complement(3488..>4175)
                     /locus_tag="VGB85_30355"
     CDS             complement(3488..>4175)
                     /locus_tag="VGB85_30355"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY0138441.1"
                     /translation="GMSDKPSIEIIGVVGHVVHYGFDAPQRVPYQMYYAYKQLPEDPQ
                     KQIGANMTLILRTQAEPHGFVAQARAAVAAVDPELPVYEVATLAELIDRSIAERRFTI
                     WLLTVFAGLALILAAVGLYAVMANSVAQRTHELGVRMALGAQPRDVVRLVVRQGLVLV
                     GIGVVLGIAGAFGLTRLMSSMLGSQVSPTDPTTFAGVTLLLVVIGVVATYIPARRATR
                     IDPMIAMRQE"
BASE COUNT          617 a         1509 c         1467 g          582 t
ORIGIN      
        1 ctcgtggcat tcgcgacgaa gtggctgtcg atgccgttct tgtggttcgg cacgggcagc
       61 atcgtcgatc ccggggcgcc gatgctgcag cccaccgata tgctggtcac ccgcgagcgc
      121 gtggtggagc cggtcgtgtt gggccgccgc ggcgcgctcg ccctgaccgc gttcggagcc
      181 gccctgtacg tcgcggcggt cgtgatgctc ctgatcggca gcccgccgaa gctgtgaata
      241 cgccgcgctc ggcgtggcag gagcagcctt ggccggggtt atggtgaaat aagcagtcct
      301 gcaaatcgac ggacccgtcg ggattatcgt tacattgcgt gtatgatgct ggccacatga
      361 gcaactatgc gatcctgatc gacagtgaac acgccaaggt ctttagcttc gacgcgggcg
      421 aggtcaccaa ccacgcgtac caccgccacg agccgcagca tcacaccggg cacacgcagg
      481 atcaggagaa gaagtccgag cactttttcc accaggtcgc ggagcacctc aagggcgcgg
      541 agcacctgct gctgatgggc tcgggcctcg gcaaggagca cttcaagacc cacctcgaga
      601 gtcaccacca cgagctggcc aagctgatcg tcggcgtcga caccgtcgat catccgaccg
      661 acaaccagct cgtcgcccac gcgcgcgagg ccttctcgaa gcacaacgtc aagctgtgag
      721 catttcaggg cccgaggcgc gcgtccggga gccggacccg ggcctcgcag ccgcgctcgc
      781 cgtcctcgcg gttggcgagc gacagggccc cgccgtgcgc ctcggcgatc tggcgcgaga
      841 gcaccagccc gatgccgctg ccgtcgggct tggtcgtgaa gaacgggacg aacaggttgg
      901 cggtctccgc gaggcccggg ccgtcgtcgc ggaccaccag ctcgatcgcc ccgccgccgg
      961 cgctccacga caccacgacc gcggccggag acctcgccgc cagcgtcgcg tcggcggcgt
     1021 tgcgcacgag gttgatcagc agctggtcga gctggtcgcc gtcggcctgg acgatcgccg
     1081 cggggcccgc tcgtacgtgc accggcacgc ggcgctcgag cgcgaccacg cggtcgaccc
     1141 acgtcgcgac ctcgaccgcg ccgggtcgcg ggggcggtaa tcgtgccagt ttggcgtatg
     1201 cgctgaggaa gcgcgcgagc gcctcggacc tgcgcgtgat gacgtcgagc ccgcgcgcga
     1261 ggtcctcgtc gaggtcgggc ggtcgcacct cggtgacgcc gtcgagctgc tcgcgcaggt
     1321 tggccgcgat cgagtggatc ggcgcgagcg agttgttgat ctcgtggccg agcacgcgga
     1381 ccaggcgttg ccaggcctgg cgctcctcct cccgcagggc ccggcgcagg tcggtcaaca
     1441 ccagcagcgt gtgcggcagg ccgcgctggc gaaactgcgt gcggcgcagc tcccacggcc
     1501 cggggccgcc ggggaacacc gtcccgatcg tgcgcggcgc ctcgccctcg aggcacgcgg
     1561 cgaggccgag cgacgcggcg tcgcggccga gcacgccggt gtccgcgtcg agcaggcgct
     1621 cggcggcgcg gttggccagg cgcagcttcc cgcccgcgtc gaaggcgagc accgcgacgt
     1681 cgatctcctc catcacggtc cgcagcagcg cgccggcctc gaaggcgccg agtcgttgtt
     1741 cgcgcaggat gtcggccagg ccgttgacct cgcgcagcac cccggacagc gcgtcgtcgg
     1801 cggggccatg gcccgtgatc cggccgcgca tcgagaagtc ctcctcgcgc agcgccgcca
     1861 gcagccccga gacgacgtgc agcgagcgga ccacgcggac gtacacgatc agcgcgcagc
     1921 ccagccacat gccggtcgcc agcgcgccga aggtccagcg caccttgacg ctgtgcgcct
     1981 cggtcaccag cagcgccagc gcgcacagca acgccggcgc gccgccggcg agcgcgagca
     2041 ggagcacccg cgcctcgtgc gagagctgtg gcccccctcg ccgctcgccg ctcacgtctc
     2101 gagcccgtgc cgcaccaggc gtcggtaaaa ggcgctgcgc gacaggccga gcttctgcgc
     2161 cgcctcgctg acgttgttgt tggcgcgcgc gagcgccagc acgatcaggt agcgctcggc
     2221 ctcgtcgagc gtcatctcgt cgaaggcggg cgcgcgcgcg gacccggggc gcaagaccag
     2281 gtcctcgacg cggatctcgg ggccgctcgc gagcagcagc gcgcgctcga tcacgtgctc
     2341 gagctcgcgg acgttgcccg gccagcggtg ctcgagcagg gcctgcttcg ccgccgcgtc
     2401 gaggtcgacc accttgcccc cgtagcgttg actccgggcg cgcaggaagt gctgcgcgag
     2461 ccgcaagatg tcctcgcggc gctctcgcag cggcggcagg tggatctcga cggtgttgag
     2521 gcggtacagc aggtcctcgc ggaacgtgcc gtcgccggtc agcttgctca gcggggcgtt
     2581 ggtggcggcg agcacccgca cgtcgacgcg ccgcagcttc gacgacccga cgcgctgcaa
     2641 ttcgccggtc tgcaacacgc gcaacagctt cgcctgctgg cccggcggca tgttggcgat
     2701 ctcgtcgagg aacagcgtgc cgccgtcggc gagctcgaag cagccgatgc gctcggcgcg
     2761 agcatcggtg aaggcgccct tgacgtggcc gaacagctcg ctctcgaaga ccccctcccc
     2821 gagcgcgccc gcgttgacgg ccacgaaggg ccgcgacgcc cgcagcgacg cggcgtgcaa
     2881 ccaccgcgcg acgacctcct tgccggtgcc gtgctcgccc gtgatcaaca ccccggcgtc
     2941 cgagggcgcg acgcgctgca tgatccgcag caccggctcc atcgcgcgcg agccggcgac
     3001 cagctcgggc aggccctcgc cggcgcgcag gcgctcgttc tcccgctcga gcttgccgct
     3061 gcgtcgcagg gcccgcgcca gctcgacctg cgtgcgcagc gtcgccagca ggcgcgtgtt
     3121 gtcccacggc ttttgaatgt agtcgcgggc gccgcggcgg atcgcctcga ccgcaccgtc
     3181 gacgctgccc cacgcggtca tcacgagcac cggcaggtgg gcgtcgagcg cggtgacccg
     3241 cgcgatcagg tcgaggccct cgcggccaga ggtcgtgtcg cgcgtgtagt tgaggtccat
     3301 caacagcgcc tcgaactcct gcgcctcgat cgccgcgagg atctcggccg gcgagcgggc
     3361 cagctgcggt gcgaagccct gcgccttgag cagcaggcgc agcgcctcga gcacgtcggc
     3421 ctgatcgtcg gcgatcagca cccgcacggg gctcacggcg cgcgtggtgg cttcggacat
     3481 cgctggctca ctcctgtcgc atggcgatca tcggatcgat gcgggtcgct cgacgcgccg
     3541 gaatatacgt cgcgaccacg ccgatgacca cgagcagcag cgtcaccccg gcaaaggtcg
     3601 tcgggtcggt cgggctgacc tggctcccga gcatcgagct catgaggcgg gtcagtccga
     3661 acgcgccggc gatgccgagc accacgccga tgccgaccag cacgaggccc tggcggacca
     3721 ccaggcgcac cacgtcgcgc ggctgcgctc ccagcgccat gcgaacgccg agctcgtggg
     3781 tgcgctgggc gaccgaattg gccatcaccg cgtagagccc gacggcggcg aggatcagcg
     3841 cgagcccggc gaacaccgtc aacagccaga tcgtgaagcg gcgctcggcg atcgaacggt
     3901 cgatgagctc cgcgagcgtc gcgacctcgt acaccggcag ctccgggtcg acggcggcga
     3961 cggccgcccg cgcctgcgcg acgaagccgt gcggctcggc ctgggtgcgc aggatcagcg
     4021 tcatgttggc cccgatctgc ttctgtggat cctcgggcaa ttgcttgtac gcgtagtaca
     4081 tctgatacgg cacccgctgc ggcgcgtcga agccgtagtg cacgacatga ccgacgacgc
     4141 cgatgatctc gatgctcggc ttgtcgctca tgccg
//
LOCUS       DASWNJ010001813         3622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_343695, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001813 DASWNJ010000000
VERSION     DASWNJ010001813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343695"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VGB85_30360"
     CDS             <1..589
                     /locus_tag="VGB85_30360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0138442.1"
                     /translation="MDIMMPGMDGWSVLQAIRADPELSGTRVVIVSMLDEKPLGMALG
                     ADDYLTKPLRREQLLGCIQRLVPVRDGYILIVEDDESLRELLQRTLIEEGWSVRIAAT
                     GRAALDLIEQSRPGLILLDLRIPEIDGFDVIASIRSRPEFVRVPITVMTALDLSRDDW
                     ERLTGRVEQVIQKGLYSRNRLMREVRRLAAHFVVR"
     gene            652..1335
                     /locus_tag="VGB85_30365"
     CDS             652..1335
                     /locus_tag="VGB85_30365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138443.1"
                     /translation="MSICTALFHFLGTAIASTVFISFGEMVLHRHLMHRQQLWGWLYR
                     VLPDLRAQFKNHAVLHHGRYYKEFDHEPDPAGKIFNVRILWGDSLRLIITFSPVMAAL
                     WFLVSWVSALTFLLMLVGHNLLWGVVHMQMHVPVVDSWIGRTAYFRFISRHHFMHHRQ
                     TAKNYNVVVPLADFVIGRVARRKVSDVREMLRLGYLEPRQAASVARMARVRDEKLVER
                     SVGLPLAAV"
     gene            complement(1346..1687)
                     /locus_tag="VGB85_30370"
     CDS             complement(1346..1687)
                     /locus_tag="VGB85_30370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138444.1"
                     /translation="MARPNDTVDEAALAKEVEVYRRLGSRTSWAFVWFAAAVLFLIVF
                     HRPQSWPGPVAEGRIAAQIVLWIAVFVAYSSNARRAERRGFILGKFGRTPAEFLAKPP
                     APEPPRDPPRS"
     gene            complement(1797..2858)
                     /locus_tag="VGB85_30375"
     CDS             complement(1797..2858)
                     /locus_tag="VGB85_30375"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY0138445.1"
                     /translation="MNNRYITLGTFVVVTLGAFGWLASQLGLGDSGGAHYTVRTPDAA
                     GLVEGNSVRIAGVEVGKVEGIHVEGNIAVIDLRIRPGTQVWADACAAVHIKGMLGEKY
                     LGIVQNGTGTPMPDGTTFACVTATVDFDNALNAAKEMVYGEDSLLPPVTRIAKRIDTM
                     TAALDEGEGLPRERLDKLLTGVEDITFTARDMLKENRDDLRALVQGGRRLVEDKRLDR
                     ILTNTDKLVTTLNKRVPDMLDQGDKILARLDKASALLDDAHVAQIDGMMDDGAAALKN
                     LRKLSDDFKDIGKELRPALKQVGPLLKNLSIIAERAADITYEKIVQMLQMEGFRVRIG
                     QGREARQVIREARKADSEE"
     gene            complement(2858..>3622)
                     /locus_tag="VGB85_30380"
     CDS             complement(2858..>3622)
                     /locus_tag="VGB85_30380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0138446.1"
                     /translation="AVVFEDVSKSFGAQCVLNGVSLVVPKGAIMVMLGPSGTGKSVLL
                     RCLVGLIKPDAGRIHVFGEDITALDENKSADRKALFALRRRFGMLFQDGALFDDMSVG
                     DNIAFPMRQHTNLREPEIRKQVNENLERVGLPGSAHKMVSELSGGMRKRAAYARAIAL
                     RPDIVLCDEPSSGLDPVMSATLDELILELHRTLGMTFIVISHDTAEARKIATHIGLLY
                     QGKLQIYDTAKVVLTSSHTAVKQFLERNTQGPIKVL"
BASE COUNT          519 a         1165 c         1270 g          668 t
ORIGIN      
        1 gatggacatc atgatgcccg ggatggacgg ctggtcggtg ctccaggcga tccgcgccga
       61 ccccgagctg tccggcacgc gcgtcgtcat cgtcagcatg ctcgacgaga agccgctcgg
      121 catggcgctc ggcgccgacg actacctgac caagccgctg cgccgcgaac aattgctcgg
      181 ctgcatccag cggctggtgc cggtgcgcga cggttacatc ctgatcgtcg aggacgacga
      241 gtcactgcgc gagctgttgc agcgcacgct gatcgaggag ggctggtccg tgcggatcgc
      301 cgcgaccgga cgcgccgccc tcgacctgat cgaacagagc cggcccggcc tcattttgtt
      361 ggacctgcgg atccccgaga tcgacggttt tgatgtgatc gccagcatcc gcagccgccc
      421 ggaattcgtg cgcgtgccga tcactgtcat gacggcgctc gacctcagtc gcgacgactg
      481 ggagcggttg accggccgcg tcgagcaggt gatccagaag gggctgtatt cgcgcaatcg
      541 cctgatgcgc gaggtccgtc ggctcgcagc gcacttcgtc gtccgttagt ccgcgcgtcg
      601 aacgccgggg tttttcctcg tcgatatggc attgttgtgc ctcgcttcgt catgtcgatc
      661 tgcaccgcgc tgttccactt cctcggtacg gcgatcgcgt cgacagtgtt catctcgttc
      721 ggggagatgg tcctccatcg ccacctgatg caccgccagc agctctgggg ctggctgtac
      781 cgcgtcttgc cggacctgcg cgcgcagttc aagaaccacg cggtcctcca ccacgggcgt
      841 tattataaag agttcgacca cgagcccgac ccggcgggca agatcttcaa cgtccgcatc
      901 ctgtggggcg actcgctgcg cctgatcatc accttctcgc cggtgatggc ggccctgtgg
      961 ttcctcgtgt cgtgggtgtc ggcgctgacc ttcttgctga tgctggtcgg ccacaacctg
     1021 ctctggggcg tggtccacat gcaaatgcat gtgcccgtcg tcgactcgtg gatcgggcgc
     1081 accgcgtact ttcgcttcat ctcgcggcac cacttcatgc accaccggca gacggccaag
     1141 aactacaacg tggtggttcc gctcgccgac ttcgtgatcg gacgggtcgc gcggcgcaag
     1201 gtctcggacg tgcgggagat gctgcggctc gggtatctcg agccgcgcca ggccgcgagc
     1261 gtggcgagga tggcgcgggt ccgggacgag aagctggtgg agcgctcggt cgggctgccg
     1321 ctcgcggcgg tgtgagggcg atccgctagc tgcgcggcgg gtctcgcggg ggttccggag
     1381 cgggcggctt cgccaggaat tcggcgggcg tgcgaccgaa ctttccgagg atgaacccgc
     1441 ggcgctcggc tctgcgggcg ttgctcgagt acgcgacaaa caccgcgatc cacaggacga
     1501 tctgcgcggc gatccgaccc tctgccaccg gcccgggcca gctctgcggg cgatgaaaca
     1561 cgatcaggaa caggaccgcg gccgcgaacc acacgaaggc ccacgaggtc cggctgccga
     1621 gtcgccgata gacctcgacc tccttcgcca gcgccgcctc gtcgacagtg tcgttcggtc
     1681 tcgccatcgc ccctccacgc ccgtggccgc gtcatgtgcc actggcgtcg agcgtgccac
     1741 gagcgggcag ggccgtccac gcggcccgtg tgtcccgtgg ggcgggcgcg cccgcgctac
     1801 tcctccgagt ccgccttcct cgcctcgcgt atgacctggc gggcctcgcg gccctggccg
     1861 atgcgaacgc ggaagccctc catctgcagc atctggacga tcttctcgta ggtgatgtcg
     1921 gcggcgcgct cggcgatgat gctgaggttc ttgagcagcg ggccgacctg cttgagggcg
     1981 ggcctcagct ccttgccgat gtccttgaag tcgtcgctga gcttgcgcag gtttttgaga
     2041 gcggcggcgc cgtcgtccat catgccgtcg atctgggcga cgtgggcgtc gtcgagcagg
     2101 gcgctggcct tgtcgaggcg ggcgaggatc ttgtcaccct ggtcgagcat gtccggcacg
     2161 cgcttgttga gcgtcgtcac cagcttgtcg gtgttggtca ggatcctgtc gaggcgtttg
     2221 tcctcgacca atcttcgacc gccctggacc agcgcgcgca ggtcgtcgcg gttctccttg
     2281 agcatgtcgc gcgcggtgaa ggtgatgtcc tcgacgccgg tgagcagctt gtcgaggcgc
     2341 tcgcgcggga ggccctcgcc ctcgtcgagc gcggcggtca tcgtgtcgat acgcttggcg
     2401 atccgggtga ccggaggcag cagcgagtcc tcgccgtaga ccatctcctt ggcggcgttg
     2461 agggcgttgt cgaagtcgac ggtcgcggtg acacacgcga aggtggtgcc gtcgggcatc
     2521 ggcgtgccgg tgccgttctg gacgatgccg aggtacttct cgccgagcat gcccttgatg
     2581 tggaccgcgg cgcaggcgtc ggcccacacc tgggtgccgg ggcggatccg gaggtcgatg
     2641 acggcgatgt tgccctcgac gtggatgccc tccaccttgc cgacctcgac gccggcgatg
     2701 cgcaccgagt tgccctccac caggccggcg gcgtcggggg tgcgcacggt gtagtgcgcg
     2761 ccgccgctgt cgcccaggcc cagctgcgag gcgagccagc cgaacgcgcc cagggtgacg
     2821 acgacgaagg ttccgagcgt gatgtatcgg ttgttcatta cagcaccttg atggggccct
     2881 gggtgtttct ctcgaggaac tgtttgacgg cggtgtggct cgaggtgagc acgaccttgg
     2941 cggtgtcgta gatctggagc ttgccttgat acaacaggcc gatgtgggtg gcgatctttc
     3001 gcgcctcggc ggtgtcgtgg ctgatgacga tgaaggtcat gccgagggtg cggtgcagct
     3061 cgaggatcag ctcgtcgagc gtggccgaca tcaccgggtc gaggcccgag gagggctcgt
     3121 cgcacaggac gatgtcgggg cgcagggcga tcgcgcgggc gtaggcggcg cgcttgcgca
     3181 tgccgccgct gagctcggag accatcttgt gggcgctgcc cggcaggccg acgcgctcga
     3241 ggttctcgtt gacctgcttg cggatctccg gctcgcgcag gttggtgtgc tggcgcatcg
     3301 ggaaggcgat gttgtcgccg acggacatgt cgtcgaacag ggcgccgtcc tggaacagca
     3361 tgccgaagcg gcggcgcaag gcgaacaggg ccttgcggtc ggcggacttg ttctcgtcga
     3421 gcgccgtgat gtcctcgccg aacacgtgga tccggccagc gtcgggctta attaaaccga
     3481 ccaggcagcg cagcaggacc gacttgccgg tgccggacgg cccgagcatc accatgatcg
     3541 cgcccttggg cacgaccagc gagacgccgt tgagcacgca ctgggcgcca aaggacttcg
     3601 atacgtcctc gaagacgacc gc
//
LOCUS       DASWNJ010001814         3158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_67600, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001814 DASWNJ010000000
VERSION     DASWNJ010001814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67600"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..2636)
                     /locus_tag="VGB85_30385"
     CDS             complement(123..2636)
                     /locus_tag="VGB85_30385"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0138447.1"
                     /translation="MLSFETGIADTAVADTRPVVRRVQGDVQAQELERGVIVGRYMVL
                     GKLGAGGMGVVYAAYDPELDRRVALKLLLPRADGGSAGRARLLREAQALAKLAHPNVV
                     AVHDVGTHGEGVWVAMELVDGHTLGAWANSQRRRWPEVLHVLTEAARGVAVAHNAGLV
                     HRDLKPDNVMIGSDGRVRVMDFGLAHGRTPAGAIDLTVAAATDSNARPELAALALRLT
                     QVGAVQGTPSYMAPEQWLGREAQTAADQFGWSVMAWELLYGERPFPGDTVMALAASVL
                     AGQRRPPARGRAVPGWLRRVIERGLMIDPRRRWPTMEALLAALQRGMRRSRAWVASTT
                     ITGIAALAAGASYKFAPREALAVTSCREAPAQPQDLWDDPQKQRVREAMHATGAPFAE
                     EVWQRVDLRLDRYATAWAAEHAAACEEHLTGTQSSHILDLRNLCLARRQSQVSQLVDI
                     FNGADRVVVENAVQAVDALPSIRFCGGIEELLTAVAPPADEQTAERVSSLRAQLERAV
                     GLEATGLNGPGLELATRVRIEAERLGYVPLTAEAALVEASALITAGKPAQADVALARA
                     ITLGLTHDLHAIAAEAAARRVFVMSDGLGHTAEALVTGGFAQALVERAGNDGRLAALL
                     SDNLGAAYERSGDRAGARTHYEHSLAIVQRGSSPGAMPVVIHHNLGGLYHDEGDLEQA
                     RFHYNEAIRLCTEILGGRHPLGAHPLAGLGDVDSREGSPDAATHHYEASLAMMEASYG
                     PDYLYLLHPLTGLGRVHELGGRSEAARHYYEHAVRIGELHQADHPLLAEALTGLASLA
                     AAAGDLSQARALGERARQMSGLIASHPAATPVALTGLDE"
     gene            complement(2796..>3158)
                     /locus_tag="VGB85_30390"
     CDS             complement(2796..>3158)
                     /locus_tag="VGB85_30390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138448.1"
                     /translation="GGCEEFPTGACDQGSPGDCSCIYPCSLDADCGTGAACLCGTKDD
                     FIFGTGCRPASCLTDADCGGFECGVSPNICRSGEALHCRTPEDDCKVDADCLPDSDRC
                     AFEAKLGRWSCSTYAFCD"
BASE COUNT          434 a         1170 c         1107 g          447 t
ORIGIN      
        1 ccacgtgtgc gggccgctgc ggtcgttgcg tgcaggacgc cgctgctcga tcgcgcacgg
       61 cctcgacatc gacgctacga atgcgcccgc gggtgctcgc caccgacatc gccgcgcgca
      121 gatcactcgt cgagacctgt cagcgccacg ggcgtcgccg cgggatgact ggcgatcagc
      181 cccgacatct gcctcgcgcg ctcgcccagc gcccgggcct gcgagaggtc gccggccgcc
      241 gcggcgaggc tggcgaggcc cgtcagcgcc tccgcgagca agggatggtc ggcctggtgg
      301 agctcgccga tccggacggc gtgctcgtag tagtggcggg cagcctcgct gcgcccgccc
      361 agctcgtgga cgcggccgag accggtgagc gggtggagca gatagaggta gtcggggccg
      421 taggacgcct ccatcatcgc cagcgaggcc tcgtagtgat gcgtcgccgc gtcggggcta
      481 ccctcgcggg aatcgacgtc gccgaggcca gcgagcggat gggcgccgag cgggtgccgt
      541 ccgccgagga tctccgtgca tagtcggatc gcctcgttat agtggaaccg ggcctgctcg
      601 aggtcaccct cgtcgtgata caggccaccg aggttgtggt ggatcacgac cggcatcgcg
      661 ccgggcgacg agcctcgctg cacgatcgcc agcgaatgct cgtagtgtgt gcgcgcgccg
      721 gcacgatctc cgctgcgctc gtatgccgcg ccgaggttgt cgctgagcag cgccgccaga
      781 cgaccgtcgt tgccggcccg ctcgaccagc gcctgggcaa atcctcctgt gacgagcgcc
      841 tccgcggtat gtccgaggcc gtcactcatc acgaacacgc gcctggccgc ggcctcggcc
      901 gcgatcgcgt gcaggtcgtg ggtgagcccg agggtgatcg cgcgggccag cgcgacatcg
      961 gcctgcgcgg gcttcccggc ggtgatcagc gcgctggcct cgacgagcgc ggcctcggcg
     1021 gtcagcggga cataaccgag ccgctccgcc tcgatgcgca cccgcgtcgc cagctcgagc
     1081 ccaggtccgt tcaagcccgt cgcctcgagc ccgaccgctc gctcgagctg ggcgcgcagc
     1141 gagctgacgc gctcggcggt ctgctcgtcg gccgggggcg cgacggctgt cagcagctcc
     1201 tcgatacccc cacagaaacg aatgctcggc agggcgtcga ccgcctgcac cgcgttctcg
     1261 acgacgacgc ggtcggcccc attaaaaatg tcgaccagct gggatacctg gctctggcgg
     1321 cgcgcgagac agaggttgcg aaggtcgagg atatggctcg actgggtgcc ggtgaggtgc
     1381 tcctcgcacg ccgcggcgtg ttcggccgcc cacgcggtcg catagcggtc gaggcgcaga
     1441 tcgacgcgtt gccacacctc ctctgcgaag ggcgcaccgg tcgcgtgcat cgcttcccgg
     1501 acccgctgtt tttgtgggtc gtcccagagg tcctgcggct gcgccggggc ctcgcggcag
     1561 ctcgtgacgg ccagcgcctc tctcggcgcg aacttgtagc tcgcgcctgc cgcgagcgct
     1621 gcgatgccgg tgatcgtcgt gctcgcgacc cacgcgcgcg agcgcctcat gccccgctgg
     1681 agcgccgcga gcagcgcctc catcgtcggc cagcggcgcc gcgggtcgat catcaggccg
     1741 cgctcgatca cgcgacgtaa ccaccccggc acggcgcgcc ctctggccgg cggccggcgc
     1801 tggccggcga gcacgctcgc cgcgagcgcc atcaccgtgt ctccggggaa cggccgctcg
     1861 ccataaagca gctcccacgc catgacgctc cagccgaact ggtcggcggc cgtctgcgcc
     1921 tcccggccga gccactgctc cggcgccatg tacgagggcg tgccctggac cgcgccgacc
     1981 tgggtcagcc gcagggccag cgcggcgagc tcgggtcgcg cgttgctgtc ggtcgcggcg
     2041 gcgacggtga ggtcgatcgc gccggcgggc gtgcgtccgt gggcgaggcc gaagtccatc
     2101 acgcgcacgc gcccgtcgct gccgatcatc acgttgtccg gcttgaggtc gcgatgcacg
     2161 agcccggcgt tgtgcgccac cgcgaccccg cgggccgcct cggtcaatac atgcaggacc
     2221 tccggccagc gccgccgctg gctgttggcc cacgcgccga gcgtgtgccc gtcgaccagc
     2281 tccatcgcga cccagacgcc ctcgccgtgg gtgccgacgt cgtgcacggc gacgacgttc
     2341 gggtgggcca gcttggccag cgcctgggcc tcgcgcagca gccgggcgcg cccggccgag
     2401 ccgccgtcgg cgcgcggcag cagcagcttg agcgcgaccc ggcggtcgag ctcggggtcg
     2461 taggccgcgt agacgacccc catcccgccc gcgccgagct tgccgagcac catgtaacgc
     2521 ccgacgatga cgccgcgctc gagctcctgc gcctggacat ccccctggac gcggcggacc
     2581 accgggcgcg tgtcggccac ggcggtatcg gcgatcccgg tctcgaagct caacatggca
     2641 cggttcgatc ataccgcccg cggtcgcgga tcggaagcag ccggactgct attcctgcgg
     2701 ccagcgccgc ggccgcacgt gccgccagcc gcgaagccgg cgccacatgc gtgggagcac
     2761 cgagataccg acaatgctcg acgtgcgagc ctcatctagt cgcagaacgc gtaggtcgag
     2821 cagctccagc gacccagctt cgcctcgaac gcgcagcggt cgctgtcggg caagcagtcc
     2881 gcgtcgacct tgcagtcgtc ctccggcgtc cgacaatgca gcgcctcccc cgagcggcaa
     2941 atattcgggc tcaccccgca ttcgaacccg ccgcagtcgg cgtcggtcag gcagctcgcg
     3001 gggcggcatc cggtgccgaa gatgaaatca tccttcgtgc cgcacaggca tgccgcgccc
     3061 gtaccgcagt ccgcgtccag cgagcacgga tatatgcacg agcaatctcc cggtgaaccc
     3121 tggtcgcagg cgccggtcgg gaactcctcg cagccgcc
//
LOCUS       DASWNJ010001815         7508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_597579, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001815 DASWNJ010000000
VERSION     DASWNJ010001815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7508
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597579"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VGB85_30395"
     CDS             <1..600
                     /locus_tag="VGB85_30395"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEY0138449.1"
                     /translation="NLAGGTHHAFAGRAGGYCVFNDSAVAARVMQAEGRVAQVVVIDC
                     DVHQGDGTAAILADDPSVFTFSIHGARNFPFTKQRSDLDIHLPDGTGDDDYLAALTEG
                     LAQALERSRAELAIFLAGADPYAGDRLGRLALSKQGLARRDELVLATCRDAGLPVAVT
                     MAGGYAQDIDDIVDIHFRTVEIAAAMAVGGVVQASSSRP"
     gene            597..1658
                     /locus_tag="VGB85_30400"
     CDS             597..1658
                     /locus_tag="VGB85_30400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0138450.1"
                     /translation="MKLLIQIPCLNERDHLGATFADLPREIAGIDSIEVLVVDDGSTD
                     GTAELAEQLGVHHVLRFPRHRGLAVAYAAGVDACLRLGADIIVNTDADNQYRGSDIAR
                     LVAPILAGRADIVVGDRRTDQLAHFSYIKRVLQRWGSNVVRRASGTAVRDATSGFRAI
                     GRKAAMTLFVHNRFTYTLETIIQAGHAGLAIANVEIECNPQTRESRLFHSLGGYVRRN
                     GAVIVRSYCMYWPVQTFGFLALLLLVLGLGLGGRFAYFYVVRWPQESGHTESLLVGVG
                     AVVLAFLVGLMALLGDLIAANRRLNEQVLVQVRELTATVTALTDGPRTPGLVRTTAAP
                     WSTAITPPTIAVGSPGDRV"
     gene            1655..2266
                     /locus_tag="VGB85_30405"
     CDS             1655..2266
                     /locus_tag="VGB85_30405"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0138451.1"
                     /translation="MSPPRVDAGNIVGNVYDKYGAPNPIVRALMRGFTGAVTDLYLGT
                     GATSVLEVGCGEALLADHLIRARRPEHFEGCDVALRPAAGIDPQIVLREASIYSLPYA
                     DASFDLVVCCEVLEHLADPPSGLAELARVARKHVLISTPWEPVWRLMNLARARYVGAL
                     GNTPGHVQHFSRRALLDLAATRLIDLRVRRPLPWTVILGDVRR"
     gene            2253..4952
                     /locus_tag="VGB85_30410"
     CDS             2253..4952
                     /locus_tag="VGB85_30410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138452.1"
                     /translation="MSAAEPSRARRLARWLPFAIVTAFGLWLLWPVPSGVMPLSADHT
                     VHLTRAHLYAQQLAGGHLVGWSPAWFFGFPLGELYPVLGDLGVLALRILSLGTLDWPQ
                     CYALLFTLVFLTQGWALLRCGRALGWGPVPGTVAGLLVLADVGAYREGGWMYTVTYGV
                     WPQALATALAWLAFAELALATRGHVPGDRSVRASDDTIAPGAPVGTEAPVPGDMSVRH
                     LVYATLAAAGALLAHPMAILMFALAVPLWLVTVGLRGADRRARLADAAVTLALVLGLG
                     AALAAWWLLPMSAHRGWMASYGWLHASLGDMARMASKGQWTYLMAPAVGHMASLGLVL
                     AALIGRGPLRFFALWTVAHWLLASTDLFWGLRLDWLSGGFQHIQYQRFLIAAKPGFFL
                     LAGAVVGAVVHLALRAWSQRHDLGARLAGIIAGLVLSGAVLSLAWQAGDVRTMMLRHQ
                     VGVVQTERVPGQPAFAADYAAFLAWARATWDARDHDYRVAFRADRNLHWFMDAPVTTR
                     TPSYKIGFTPGDNFVHKPESGDPALLRRLGVRFVVSTGSTPPAGGTLVERFGKIHVFE
                     LAGGEPLAHLEGGGEVTFENPEIGGEGLRLRVRGATDETRLVIHVAGYPRWELRWQPD
                     GGAPEVVEWFEAPVLGDSPIATQTQRRGGELRGGKALGDDGREPTLIAAAARDGVYTL
                     RYVRWRGVDVVGLVLALLGALGLFVLTRRGDALLSRVRPLARRLVPPVLLLVLAAGLV
                     LAVVRHRQGSAREHDLASARLRRGEAQAHGVIAAPLKTDMIVFPAVLVRPDRGGESEV
                     VYPGVQLGATLDGWYALDDDDAKQRRRGSHRLYITARAAGSEPWTALADLPVTHRPDR
                     RRLEHIAVPAALQGIPVDVQVSVETTGEAPPKFGFDLTLPGAP"
     gene            4998..6491
                     /locus_tag="VGB85_30415"
     CDS             4998..6491
                     /locus_tag="VGB85_30415"
                     /inference="COORDINATES: protein motif:HMM:NF024573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3696 domain-containing protein"
                     /protein_id="HEY0138453.1"
                     /translation="MLRSLRLQNFRSLRDTGDVELRPIVLLVGANSSGKSSFLRFFPL
                     LKQTAQANSRSPLLWFREERGLVDFRDFENTLSRGAPEPEIIIAGALDEISINPAGES
                     ATCRFRSHIAAKNGTSYLRSFHFQCLDDTLVANFDASRRVTSMELQGADAIAHMDPSR
                     REVALDSLIPNFSDTLVSTTAHIQLSDKIWERVHGRTGSDKIDDIALNVDYGSAEHVQ
                     QAMLEISGGAKRWRENIQALKPTDPYIQEIKRLAFLRAVGGRDYWNPMETLASSLHGL
                     ANGVRYIGPFRRKPDRFYRQTEVSVDEIDATGSNLAMFLYSLDEPGRVGFSSWVYEHF
                     GFHVRAERREAQVVLRIDTGSESHFDLTDMGFGISQLLPVAAQCWLSSQGKPRRQGHA
                     PSVVAIEQPELHLHPHHQFQLADMFLGLATAAKETHQRLTLCIETHSEAMIHRFGELV
                     AEDPRRRDDITILFFEKAADSDVSNVRQIEFDAEGVLQNWPIGFFRG"
     gene            6496..7464
                     /locus_tag="VGB85_30420"
     CDS             6496..7464
                     /locus_tag="VGB85_30420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138454.1"
                     /translation="MLIVLDDAVLPADAAALSSAGRDALENLAKGAYEGNHMVTGSRK
                     LLLRLKRLKAVLGAPTCDRFTYLSFEVNDAARLRHEVRSLLIVHEVGYAPRIDRPAVA
                     LPGDPIVFRVGLDYFARTARIQPTQVIGEDQRDADFFSRLASAFALRHPGIVSATVPV
                     AGGGVNTDRVLIAKAPHQIVLCVVDTDQDTPQAPPKDTARRAIDAGETLRLQAHVADV
                     YVLRCRELENMLPKTLVLAALPDTGERQERDKVERAAQFLGRFDYTDLKAVASKHILK
                     WVSIYLERVPDQDLAAHCFQQDIHPAMAELAATLWSFGLAPVRGST"
BASE COUNT         1117 a         2747 c         2397 g         1247 t
ORIGIN      
        1 aacctcgccg gcggcacgca ccacgccttc gccgggcgcg ccggcggtta ctgcgtgttc
       61 aacgacagcg ccgtcgcggc gcgcgtgatg caggccgagg gtcgcgtcgc gcaggtcgtc
      121 gtcatcgact gcgacgtaca tcagggcgac ggcaccgcgg cgatcctcgc cgacgacccg
      181 agcgtgttca ccttttcgat ccacggcgcg cgcaacttcc cgttcaccaa gcagcgcagc
      241 gacctcgaca tccacctgcc cgacggcacc ggcgacgacg actacctcgc cgcgctgacc
      301 gagggcctcg cgcaggcgct cgagcgctcg cgggccgagc tggcgatctt cctcgccggc
      361 gccgacccgt acgccggcga ccgcctcggc cggctcgccc tgagcaagca gggcctggcg
      421 cgccgcgacg agctggtcct tgcgacatgt cgcgacgccg gtctgccggt cgccgtcacc
      481 atggccggtg gttacgccca ggacatcgac gacatcgtcg acatccactt ccggaccgtc
      541 gagatcgcag ctgccatggc cgtcggtggc gtggtacaag cgtcgtcttc gcggccgtga
      601 agctcctcat ccagatcccc tgcctcaacg agcgggacca cctcggggcg accttcgccg
      661 acctcccgcg cgagatcgcc ggcatcgaca gcatcgaggt cctcgtcgtc gacgacggca
      721 gcacggacgg caccgcggag ctcgccgagc agctcggcgt ccaccatgtg ctccggttcc
      781 cgcggcaccg cggcctcgcc gtcgcctacg cggccggcgt cgacgcctgc ctgcgcctcg
      841 gcgccgacat catcgtcaac accgacgccg ataatcaata ccgcggctcc gacatcgcgc
      901 gcctggtcgc gccgatcctc gccggccgcg cggacatcgt cgtcggcgac cgccgcaccg
      961 accagctcgc gcatttttct tatatcaagc gcgtgctgca gcgctggggg tccaacgtcg
     1021 tgcgtcgcgc cagcggcacg gccgtgcgcg acgcgaccag cggctttcgt gcgatcggcc
     1081 gcaaggccgc gatgaccctg ttcgtccaca accgcttcac ctacaccctc gagaccatta
     1141 tccaggccgg acacgccggc ctggcgatcg cgaacgtcga gatcgagtgc aacccgcaga
     1201 cccgcgagtc gcggctgttc cactcgctcg gcggctacgt gcgccgcaac ggcgccgtca
     1261 tcgtccggtc gtattgcatg tactggccgg tccagacctt tggcttcctc gcgctgctgc
     1321 tgctggtgct cggcctcggc ctcggcgggc gcttcgcgta cttctatgtc gtgcgctggc
     1381 cgcaggagag cgggcacacc gagtcgctgc tcgtgggagt cggcgccgtc gtgctcgcct
     1441 tcctcgtcgg cctcatggcc ctgctcggcg acctgatcgc cgccaaccgg cggctcaacg
     1501 agcaggtgct cgtgcaagtg cgcgagctga ccgccacggt caccgcgctg accgacggcc
     1561 cgcgcacgcc cggcctcgtg cgcaccaccg ccgctccatg gagcaccgcg atcaccccgc
     1621 cgacgatcgc cgtcggctcg cccggagacc gcgtatgagc ccgccgcggg tcgacgccgg
     1681 caacatcgtc ggcaacgtct acgacaagta cggcgcgccc aacccgatcg tgcgcgccct
     1741 gatgcgcggc ttcaccggcg ccgtcaccga cctgtacctc gggacaggtg cgaccagcgt
     1801 gctcgaggtc ggctgcggcg aggccctgct cgccgaccat ttgataaggg cccgccggcc
     1861 ggagcacttc gagggctgtg atgtcgcact gaggcccgcg gccggcatcg acccgcagat
     1921 cgtgctgcgc gaggcgtcga tctatagctt gccctacgcc gacgccagct tcgacctcgt
     1981 cgtctgctgc gaggtcctcg agcacctcgc ggacccaccc tcgggcctcg ccgagctcgc
     2041 ccgcgtcgcc cgcaagcacg tgctgatcag caccccgtgg gagccggtgt ggcggctgat
     2101 gaacctcgcc cgggcccgct acgtcggcgc cctcggcaac acgcccgggc acgtgcagca
     2161 cttcagccgc cgcgccctgc tcgacctcgc cgcgacccgt ttgatagatc ttcgggtccg
     2221 tcggcccctc ccttggacgg tgatcctggg cgatgtccgc cgctgagccc tcacgcgcgc
     2281 gacgcctcgc gcgctggctg ccctttgcga tcgtcacggc gttcggcctg tggctgctgt
     2341 ggccggtgcc gtccggcgtg atgccgctgt cggccgacca cacggtgcac ctgacgcgcg
     2401 cgcacctgta cgcccagcag ctcgccggcg gccacctcgt cggctggagc cccgcgtggt
     2461 tcttcggctt cccgctcggc gagctctacc cggtgctcgg cgacctcggc gtgctcgcgc
     2521 tgcgcatcct ctccctcggc acgctcgact ggccgcagtg ctacgcgctg ctgttcacgc
     2581 tcgtgttcct gacccagggc tgggccctgc tgcgctgcgg tcgcgcgctc ggctggggcc
     2641 ccgtgcccgg caccgtcgcc ggcctgctcg tgctcgccga cgtcggcgcc tatcgtgagg
     2701 gcggctggat gtacacggtc acctacggcg tgtggccgca ggcgctggcg accgcgctcg
     2761 cctggctggc gttcgccgag ctcgcgctgg cgacgagagg acatgtcccc ggagacaggt
     2821 cggtgcgcgc cagcgacgat acgatcgccc ccggcgcgcc cgtcggcacc gaagcacctg
     2881 tccccgggga catgtcggtg cggcacctcg tgtacgcgac gctcgcggcc gccggggccc
     2941 tgctcgcgca cccgatggcg atcctgatgt tcgcgctcgc ggtcccgctg tggctcgtca
     3001 ccgtcggcct gcgcggcgcg gaccgccggg cccggctcgc ggacgcggcc gtcacgctcg
     3061 ccctcgtgct cggcctcggc gccgcgctgg cggcgtggtg gttgctgccg atgagcgccc
     3121 accgcggctg gatggccagc tacgggtggc tgcacgcctc cctgggcgac atggcgcgca
     3181 tggcgagcaa gggccagtgg acctacctga tggcccccgc cgtcggccac atggccagcc
     3241 tcggcctcgt gctcgcggcg ctgatcggcc ggggaccgct gcgcttcttc gccctgtgga
     3301 ccgtcgccca ctggctgctc gccagcaccg acctgttctg gggcctgcgc ctcgactggc
     3361 tcagcggcgg cttccagcac atccagtacc agcggttcct gatcgccgcc aagccgggct
     3421 tcttcctgct cgccggggcg gtcgtcggcg cggtcgttca cctggccctt cgggcatggt
     3481 cgcagcgaca tgacctcggg gccaggctcg ccggcatcat cgccgggctg gtgctcagcg
     3541 gcgccgtgct ctcgctggcc tggcaggccg gcgacgtccg gacgatgatg ctgaggcacc
     3601 aggtcggcgt cgtgcagacc gagcgcgtcc ccgggcagcc ggcgttcgcg gccgactacg
     3661 cggccttcct cgcgtgggcg cgcgccacct gggacgcccg cgaccacgac taccgcgtgg
     3721 cgtttcgcgc cgaccgcaac ctgcactggt tcatggacgc gccggtcacc acgcgcaccc
     3781 cgagctacaa gatcggtttc acgccgggtg acaacttcgt ccacaagccc gagtcgggcg
     3841 acccggccct gctgcgccgc ctcggcgtgc gcttcgtggt cagcaccggc agcacgccgc
     3901 ccgccggtgg cacgctggtc gaacgctttg gaaagatcca cgtgttcgag ctcgcaggcg
     3961 gcgagccgct cgcgcacctc gagggcggcg gcgaggtcac ttttgaaaac cccgagatcg
     4021 gcggcgaggg cctgcgcctg cgcgtccgcg gcgcgaccga tgaaacgcgg ctggtcatcc
     4081 acgtcgccgg ttatccgcgc tgggagctcc gctggcagcc ggacggcggc gcgcccgagg
     4141 tcgtcgagtg gttcgaggcg cctgtcctcg gggacagccc gatcgccacc cagacgcagc
     4201 gccgcggcgg tgagctgcgc ggcggcaagg cgctcggcga cgacggccgc gagccgacgc
     4261 tgatcgcagc ggccgcgcgc gacggcgtgt acaccctgcg ttacgtccgc tggcgcggcg
     4321 tcgacgtcgt cggcctcgtg ctcgcgctgc tcggcgcgct cggattgttc gtgctcacgc
     4381 gccgcggtga cgccctgctg tcccgcgtgc gcccgctggc ccggcgcctg gtcccgccgg
     4441 tcctgctgct cgtgctcgcc gccggcctcg tgctcgccgt cgtgcgccac cgccagggct
     4501 cggcccgcga gcacgacctc gccagcgcgc gcctgcgtcg cggcgaggcg caggcgcacg
     4561 gcgtgatcgc agcgccgctc aagaccgaca tgatcgtgtt cccggccgtg ctcgtgcgac
     4621 ccgatcgcgg cggtgagagc gaggtcgtct accccggtgt gcagctcggc gcgaccctcg
     4681 acggctggta cgccctcgac gacgacgacg ccaagcagcg ccgccgcggc agccatcgcc
     4741 tgtacatcac cgcgcgagcg gcgggcagcg agccgtggac cgccctcgcc gacctcccgg
     4801 tcacgcaccg ccccgaccgc cgccgcctcg agcacatcgc cgtgcccgcc gcgttgcagg
     4861 gcatccccgt cgacgtccag gtcagcgtcg agaccaccgg cgaggcccct cccaaattcg
     4921 gcttcgacct cacgctgccc ggagcgccct gagcaccgcg ccttgctcgc accgacgcgc
     4981 gcgtggtacg aagagccgtg ctacggagcc tccgcctcca gaatttccgc agcctgcgcg
     5041 acacgggcga cgtggaattg cggccgatcg tcctgctcgt gggcgccaac agctcgggca
     5101 agagctcctt tctgcgcttc tttccgctcc tcaaacaaac agcgcaggcg aacagccgca
     5161 gcccgctcct gtggttccgc gaggaacgtg ggctagtcga tttccgcgac ttcgagaaca
     5221 cgctgtcgcg gggcgctcca gaaccggaga tcatcatcgc tggcgcgctc gacgagatct
     5281 cgatcaaccc agcaggcgaa tccgccacgt gccgcttccg gtcccatatc gccgccaaga
     5341 acgggacctc ctatctccgc agtttccatt tccaatgcct ggatgacacg cttgtcgcca
     5401 atttcgacgc gagccgccgt gtcacttcca tggagttaca gggcgcagac gctatcgccc
     5461 acatggatcc aagcaggcga gaggtcgcgc tcgacagcct catacctaac ttcagcgaca
     5521 ccctcgtgag cacaacagct cacatacagc tatccgacaa gatctgggaa cgcgtgcatg
     5581 gccgaacagg cagcgacaag atcgatgata tcgctttgaa cgtcgactac ggctcggccg
     5641 aacatgtcca acaggccatg ctcgagatct ccggcggagc gaaacggtgg cgggaaaaca
     5701 tccaagcgct caagcctacc gatccataca ttcaagagat caagcgcctc gcctttctaa
     5761 gggccgtcgg gggccgcgac tattggaacc cgatggagac actggcgtcg tcacttcatg
     5821 gccttgcgaa cggcgtgaga tacattggac cttttcggcg caagccagac cgcttctacc
     5881 gtcagacgga ggtctccgtg gatgagatcg acgcgaccgg aagcaacttg gcgatgttct
     5941 tatattctct tgacgagcca ggtcgcgtcg ggttctcaag ctgggtatac gaacatttcg
     6001 gtttccatgt gcgcgccgag cgccgcgagg ctcaggtcgt cttgcgcatc gacacaggta
     6061 gcgaatcgca cttcgacctg acggacatgg gtttcggcat atctcagttg ctccctgtgg
     6121 cagcccagtg ctggctctcg agccaaggta agcctcgacg acaggggcac gcaccgtcgg
     6181 tcgtcgccat cgagcagcct gagctacatc ttcatcccca ccaccagttt cagctcgcgg
     6241 acatgttctt gggcctggcg accgcggcca aggaaacgca tcagcggctc acgctctgca
     6301 tcgagactca cagcgaggcg atgatccatc gcttcggcga gttagtggca gaggaccctc
     6361 ggcgccggga cgacatcact atcctgttct tcgagaaggc cgcggacagc gacgtgagca
     6421 acgttcggca gatcgagttc gacgccgaag gtgtgcttca gaactggcct atcggcttct
     6481 tccgcggctg agcgcatgct gatcgtgctc gatgacgccg tcttgcccgc cgatgctgcg
     6541 gcgctctcga gcgcgggccg agatgccctg gagaacttgg cgaaaggggc ctacgagggc
     6601 aaccacatgg tcactggctc gcgcaagctg ttgctgcgac tcaagcgcct caaggcagtc
     6661 ctcggcgcac cgacctgcga tcgtttcacc tacctcagct tcgaggtaaa cgatgcagcc
     6721 cggcttcgcc acgaggtccg ctctctcctc atcgtccatg aggtcgggta cgcgcctcgc
     6781 atcgatagac cagcggtcgc tctgcctggg gatcctatcg tgtttcgggt ggggcttgac
     6841 tacttcgcac gtacggcgcg catccagccg acgcaggtca ttggggagga ccagcgcgac
     6901 gcagactttt ttagccgtct cgccagcgcg ttcgctttac gtcatccggg catcgtcagt
     6961 gcgaccgttc cggtcgctgg gggcggtgtc aacacggatc gagttctcat tgcgaaagct
     7021 ccccaccaga ttgtgctctg tgtcgtcgac acagatcagg acacgcccca agcgccaccc
     7081 aaagacaccg cgagacgagc catcgacgct ggtgaaacct tgaggctaca agcgcatgtt
     7141 gccgacgttt acgtgctccg atgtcgcgag ctggaaaata tgctcccaaa gacgcttgta
     7201 ctcgctgcgc ttccagatac cggagagcgc caagaacgcg ataaagtcga gcgcgcagca
     7261 caatttcttg gtcgattcga ttataccgac ctaaaagcag tagctagcaa gcacattctg
     7321 aaatgggtca gcatttatct ggagcgtgtg cctgaccagg atctggcagc acactgcttt
     7381 cagcaagaca tccatccagc catggccgaa ctggccgcga ctctctggag cttcggtctc
     7441 gccccggttc gcggctcgac ctgagatcca gcgctgacct cgcctgccat ctcccgaaca
     7501 tcagcgat
//
LOCUS       DASWNJ010001816         1853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_67617, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001816 DASWNJ010000000
VERSION     DASWNJ010001816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67617"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VGB85_30425"
     CDS             complement(<1..239)
                     /locus_tag="VGB85_30425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138455.1"
                     /translation="MPPENPESEDDRAIDPADASHASATPGSVSAPDGTAPAPGPTGT
                     QPGDFPLPPLGTQPGEPPARTGLTLPGDPPTGFPTS"
     gene            complement(499..1050)
                     /locus_tag="VGB85_30430"
     CDS             complement(499..1050)
                     /locus_tag="VGB85_30430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY0138456.1"
                     /translation="MATLVLFNKPFGVQTRFTDPAGRATLADYIPVADVYPCGRLDAD
                     SEGLLVLTDDGRLQHRLSDPRHKQPKTYWAQVEGIPDAGAITRLERGVSLKDGPTAPA
                     QARVMAAPALWDRVPPIRYRAAIPTAWIELTIHEGRNRQVRRMTAAVGFPTLRLVRWS
                     VGPWTLEGLAPGAWRSLRVGAGI"
     gene            1159..>1853
                     /locus_tag="VGB85_30435"
     CDS             1159..>1853
                     /locus_tag="VGB85_30435"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0138457.1"
                     /translation="MSSTSFAVDLQDIHFVLFDQLEMDRKLGQHGKYADLDREVYEAT
                     LTESKRIAEEVLGPINKTGDRVGCKLDADGNVTTPPGYKEAWKILADGGWIAVSAPSE
                     LGGGGMPFTMAMIVNEILCGASMAFMMYPGLTAGAARVILEHAPEHLRETFARNMFSG
                     KWGGTMCLTEAGAGSSVGDNRSKATPTDNPDEPGTYLLEGEKIFISGGDQDLTENIVH
                     LVLARTPGAPRGT"
BASE COUNT          284 a          639 c          677 g          253 t
ORIGIN      
        1 gacgtcggga agccggtcgg cggatccccc gggagcgtca gacccgtgcg cgccgggggc
       61 tcgcccggct gcgtccccag tggaggcagt gggaagtccc cgggctgcgt gccggtgggt
      121 ccgggcgcgg gcgccgtgcc atcgggcgcc gaaacggagc caggggtcgc ggacgcatgc
      181 gacgcgtccg ccgggtcgat cgctcgatcg tcctcggact ccgggttctc cggtggcacc
      241 gcgctcagtg gccgcgatgg cgcgtcgtcg cttgtgcggc tcaggtcgcg gctgccttcg
      301 ggagtggtgc ccatcggacc gggagacgct atcacggcga tcgcgggcgg aaaagctgaa
      361 cacttccccc atgtccgcgg gctctggccc agtttggcgg ggttttgccc cggcgatcgg
      421 cccgccgagg ccgcgcgggg gccggcgcgc accgacgggg acacccgggc agccgtcgag
      481 gcccgcgatg gcgaccgttc agatcccggc tcccacgcgc agcgatctcc atgcgcccgg
      541 cgcgagaccc tcgagggtcc acggccccac ggaccagcgc accaggcgaa gcgtgggaaa
      601 cccgaccgca gcggtcatgc gtcgcacctg tcggttgcgt ccctcgtgga tcgtcagctc
      661 gatccatgcg gtcgggatcg ccgcgcgata gcggatcggg ggcacgcggt cccacagcgc
      721 cggggccgcc atcacgcgag cctgcgcggg cgcggtcggt ccgtccttga gtgacacgcc
      781 gcgctccagc cgcgtgatcg ccccggcgtc cgggatcccc tcgacctggg cccagtaggt
      841 cttgggctgc ttgtgtcgcg ggtcgctgag gcgatgttgc aagcggccgt cgtcggtgag
      901 cacgagcaag ccctcgctgt cggcgtcgag gcgaccgcag gggtacacgt cggcgacggg
      961 gatgtagtcc gccagcgtcg cgcgacccgc cggatcggtg aagcgcgtct gtaccccgaa
     1021 gggcttgttg aagaggacca gcgtggccac agcgctcgca ggttttgacc gggcgcggtc
     1081 aaaagcgcaa gggcgcgtgt gtgtggacac gagggcccgc ccctggacgc acctggcgcg
     1141 aggaagtatg cagaagcggt gagcagcacc agcttcgccg tcgatctgca ggatatccac
     1201 ttcgtcttgt tcgaccagct cgagatggac cgaaagctcg gccaacacgg caagtacgcc
     1261 gacctcgacc gcgaggtgta cgaggcgacc ctcaccgaga gcaagcggat cgccgaggag
     1321 gtcctgggcc cgatcaacaa gaccggcgac cgcgtcggtt gcaagctcga cgccgacggc
     1381 aacgtgacga cgccgcccgg ctacaaggag gcctggaaga tcctcgcgga cggcggctgg
     1441 atcgcggtct cggccccgag cgagctcggc ggcggcggca tgccgttcac gatggcgatg
     1501 atcgtcaacg agatcctgtg cggcgcctcg atggccttca tgatgtaccc cgggctgacc
     1561 gccggcgcgg cgcgggtgat cctcgagcac gcgcccgagc acctgcgcga gaccttcgcc
     1621 cgcaacatgt tcagcggcaa gtggggcggc acgatgtgcc tcaccgaggc cggcgccggc
     1681 agctcggtcg gcgacaaccg cagcaaggcg acgccgaccg acaaccccga cgagcccggc
     1741 acctacctgc tcgagggcga gaagatcttc atcagcggcg gcgatcagga tctcacggag
     1801 aacatcgtcc acctggtcct ggcgcgcact ccgggcgcgc cgcgcgggac caa
//
LOCUS       DASWNJ010001817         4224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_491864, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001817 DASWNJ010000000
VERSION     DASWNJ010001817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491864"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..585
                     /locus_tag="VGB85_30440"
     CDS             130..585
                     /locus_tag="VGB85_30440"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY0138458.1"
                     /translation="MARFTRTLEVAAPASVLWAFHERPDAFSLLMPPWESVEVLQPPR
                     SLAVGTQVIVRQRLGPLRFTITAEHVEYEHGHMFADRMSGGPFKSWLHRHIVEPTATG
                     SRLIDDIEYELVGGKLAQALVGRMAERRLDRMFAHRHAVTRRVCETGVA"
     gene            641..1714
                     /locus_tag="VGB85_30445"
     CDS             641..1714
                     /locus_tag="VGB85_30445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138459.1"
                     /translation="MSATGPKLAAGVAVLLAAAPASAAPDDPDPVLRWRAPEGCPAEA
                     RLRGRIDGLLAGVAPGLTADAEVHAPMPDDPRWRLDLQIRWARGHDDRTLHASSCDAL
                     ADATVVLVAVLAAPLALADRFTPPVIVTPPGPTPLVPAAPAPVAPAPEILAPTVEPDT
                     AGPFERPVRVRPAPPRFVRRRGPFARVAAIAGYGVLPRFDLGIQLAAGALLPRLRVEG
                     ALLLLPPQTQVLADGSGRGAVPAFASAAVRVCPRFLARPVDLSLCGALEGGISWSRSV
                     GVDLERRATGPWLAFALGPALDWWFSPQVALHLAVEAVGAPVSTTYSLGPQVIAGGRP
                     FGVRGGFGLTFALAQQKPGRPEK"
     gene            1722..2861
                     /locus_tag="VGB85_30450"
     CDS             1722..2861
                     /locus_tag="VGB85_30450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0138460.1"
                     /translation="MDSHPHLTVPARADLGDLAAFGRLYRDTAGLVRRSLARLGVIEA
                     ALDDATQDVFITAYRRRDEFDSARPIEPWLLGIARKVAFRYRRTVARGQRKLTALQHV
                     AEAPREQLAAHVEARRFLASFLEELRDERREVFVLGELYGLTGPEIAARLKIPVDTAY
                     TRLRAGRRQLELALLRSADDEPAQTSAALQRGWLLLAPRLGDSSGAGWLLLALGKAKL
                     ALGVTAVAATVALVVIPRDAPAPAPAIAPKIAAKPEPPASPTAPARLQAPPQPPDEAT
                     PPAPTLTPSRGQAPTRPPSDPLGADRLTAALALLQAGDPAGALAEADRHAREHPTSPL
                     AEARTLTRVRALCALGRDEQARSEATSLDRAGPAGEALAGTCAAP"
     gene            2915..4201
                     /locus_tag="VGB85_30455"
     CDS             2915..4201
                     /locus_tag="VGB85_30455"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0138461.1"
                     /translation="MTLILDLKTYLLLLVPLASGCTINQDLLTAGEQETTGTTTDPGD
                     PTGEDEPTGAATDPGDPTGEDEPTGCGETDCPQIYEPSNCLRELAWNGQLIGLTRQTV
                     AVDDHETVRTILAHVDPDTHALTPFAEGLVGTDSRTMVLGPDGAIYTGGNKGALFEGD
                     ATPQWLQKYSPEGVLLWSVRPEDNGETVSTAVDLALLGDELVVIGEEGELRGRSTASG
                     ALLWELPATDGARFRDAEVDGEGALHVIASAPSGSEDMPEPGWSIRKYAADRTLLWED
                     IELAAEPGGFVGSSGFAFDGAGGLILALTQYGSDPKVRDTRLRKYDTMGTEVWTRALP
                     GLEPQESIDRFYTRPGGGVIAIGPGLGDDNPSGAVTIAFDPEGALLWVDRQAHPELSD
                     VRNNAAVIVDGTVMVSGCGLGPPSSDTTAWMLKLAL"
BASE COUNT          556 a         1686 c         1409 g          573 t
ORIGIN      
        1 caagctcggc atctgcggcg agtgcaacgc cgacaccgac tgcggcgcga accagatctg
       61 ctccaccccg ctcgtcaacc tcgacaccaa ccagctgatc ggctcgacct gcgtctgaaa
      121 cccatcctca tggcccgctt cacccgcacc ctcgaggtcg ccgcgccggc ttcggtgctg
      181 tgggccttcc acgagcgccc cgacgcgttc tcgctgctga tgccgccgtg ggagtccgtc
      241 gaggtgttgc agccgccgcg gtcgctcgcg gtcggcacgc aggtgatcgt ccgccagcgg
      301 ctgggtcccc tgcgcttcac gatcaccgcc gagcacgtcg aatacgaaca tggccacatg
      361 ttcgcggacc gtatgtcggg cggccctttt aaaagttggt tgcaccgcca catcgtcgag
      421 ccgaccgcca ccggctcgcg cctgatcgac gacatcgagt acgagctcgt cggcggcaag
      481 ctcgcgcagg cgctcgtcgg tcgcatggcc gagcgccgcc tcgatcgcat gttcgcccac
      541 cgccacgcgg tcacccgccg cgtgtgcgag accggcgtcg cgtagcggtc gcgcctccac
      601 acgccttgcc aggccccgcg aagcgggcta tctcgtgcgg atgtctgcga ctggcccgaa
      661 gctcgctgca ggcgtcgctg tgctgcttgc agccgcccct gcgagcgccg ctcccgacga
      721 tcccgacccg gtgctgcgct ggcgcgcgcc ggagggctgc cccgcggagg cccggctgcg
      781 cgggcgcatc gacggcctgc tcgccggtgt cgcgcccggc ctgaccgccg acgccgaggt
      841 ccacgcgccg atgcccgacg acccgcgctg gcgcctcgac ctgcagatcc gctgggctcg
      901 cgggcacgac gaccgcaccc tgcacgcgag cagctgcgac gcgctggccg acgcgacggt
      961 cgtgctcgtc gcggtgctcg ccgccccgct cgcgctcgcg gatcgcttca cgccgccggt
     1021 gatcgtgacg cctccgggcc ccacgccgct cgtgcccgcc gctccggcgc cggtcgcccc
     1081 cgcgccggag atcctcgcgc cgaccgtcga gccggacacc gccgggccct tcgagcgacc
     1141 cgtccgcgtg aggccggccc cgcctcgctt cgtgcgtcgt cgtggcccgt tcgcccgggt
     1201 cgcggcgatc gccggttacg gggtcctgcc gcgcttcgac ctcggcatcc agctcgccgc
     1261 cggcgccctg ctgccacgcc tgcgcgtcga gggtgcgctg ctgttgctgc ccccgcagac
     1321 gcaggtgctc gccgacggca gcggtcgcgg cgcagtgccg gcgttcgcca gcgcggccgt
     1381 gcgggtctgt ccgcgcttcc tcgcgcggcc ggtcgacctc tcgctgtgcg gcgcgctcga
     1441 gggcggcatc agctggagtc gcagcgtcgg cgtcgacctc gagcgccgcg cgaccggacc
     1501 ctggctcgcg ttcgcgctcg gtcccgcgct cgactggtgg ttctcgccgc aggtcgccct
     1561 gcacctcgcg gtcgaggccg tcggcgcgcc ggtgtcgacc acctactcgc tcggcccgca
     1621 ggtcatcgcc ggaggccggc cattcggcgt tcgcggcggt tttggcctga ccttcgcgct
     1681 cgcgcaacag aaacccgggc ggccggagaa gtagcgggac gatggacagc cacccgcacc
     1741 tgaccgtccc ggcgcgggcc gacctcggcg acctcgcggc ctttggccgg ctctaccgcg
     1801 acaccgccgg gctggtccgc cgatcgctcg cgcggctcgg ggtgatcgag gccgcgctcg
     1861 atgacgcgac ccaggacgtg ttcatcaccg cctaccgacg tcgcgacgag ttcgacagcg
     1921 cgcggccgat cgagccgtgg ctgctgggca tcgcccgcaa ggtcgcgttt cgttaccgcc
     1981 gcaccgtcgc ccgcggccag cgcaagctca ccgccctgca gcacgtcgcc gaggccccgc
     2041 gcgagcagct cgccgcgcac gtcgaggccc gccgcttcct cgcgtcgttc ctcgaggagc
     2101 tgcgcgacga gcgacgcgag gtgttcgtgc tcggtgagct ctacggcctg accggcccgg
     2161 agatcgccgc gcgcctgaag atccccgtcg acaccgccta cacccgcctg cgcgccggtc
     2221 gacgccagct cgagctggcc ctcctgcgca gcgccgacga cgagcccgcg cagaccagcg
     2281 ccgcgctgca acgcggctgg ctgctgctcg ccccgcgcct cggcgactcg agcggcgccg
     2341 gctggctgct gctcgcgctc ggcaaggcca agctggcgct cggcgtcacc gcggtcgcgg
     2401 cgaccgtcgc cctcgtcgtc atcccacgtg acgcgcccgc gcccgcccct gcgatcgccc
     2461 cgaagatcgc cgcgaagccg gagccacccg cgtccccgac cgcgcccgcg cggctccagg
     2521 cgcccccgca gccccccgac gaagccacgc cgcctgcacc cacgctcacc ccctcgcgcg
     2581 gccaggcgcc gaccagaccg ccaagcgacc cgctcggcgc cgaccgcctg accgccgcgc
     2641 tcgcgctgct ccaggccggt gaccccgcgg gcgcgctcgc cgaggccgac cgccacgccc
     2701 gcgagcaccc gaccagcccg ctcgcggagg cgcgaacctt gacgcgtgtg cgcgccctgt
     2761 gtgcgctcgg tcgtgacgag caggcccgca gcgaggcgac ctcgctcgac cgcgcgggtc
     2821 cggccggcga ggccctcgcc ggcacctgcg ccgcgccgtg aagaaattct cctgctgcga
     2881 aacagaaccc gacctcgccg gagaagctga gcgcatgacg cttattcttg atctcaaaac
     2941 ttacctcttg ctgctcgtcc ccctggcgag cggctgcacg atcaaccagg acctgctgac
     3001 cgccggcgag caggagacca ccgggaccac caccgacccc ggagacccga ccggcgagga
     3061 cgagcccacc ggggccgcca cggaccccgg cgacccgacc ggcgaggacg agcccaccgg
     3121 ttgtggtgag acggactgcc cgcaaatcta tgagccctcg aattgcctca gagagctcgc
     3181 ctggaatggt cagctgatcg gcctgacgcg ccagacggtc gccgtcgacg accacgagac
     3241 cgtgcggacg atcctcgccc acgtcgaccc cgacacccat gccctgactc ccttcgccga
     3301 gggactggtc ggcaccgaca gccgcaccat ggtcctcggc ccggacggcg ccatctacac
     3361 cggcggtaac aagggcgccc tcttcgaggg cgacgcgacg ccgcagtggt tacaaaagta
     3421 ctcgccggag ggcgtgctgc tgtggtccgt caggcccgag gacaacggcg agaccgtcag
     3481 cacggccgtc gacctcgccc tgctcggcga cgagctggta gtcatcggtg aggaaggcga
     3541 gctgcgcggt cggtcgacgg ccagcggcgc cttgctgtgg gagctcccgg ctactgacgg
     3601 cgccaggttc cgtgacgccg aggtcgacgg tgaaggtgcc ctccacgtca tcgcaagcgc
     3661 cccgtcgggc tccgaagata tgccggagcc cggctggtcg atccgcaaat acgccgccga
     3721 tcgcaccctg ctgtgggaag atatcgagct cgccgccgaa ccgggcgggt tcgtcggctc
     3781 ctccggcttc gccttcgacg gggccggcgg gctgatcctc gccctgacgc agtacggctc
     3841 cgaccccaag gtccgcgaca cccgcctgcg caagtacgac accatgggca ccgaggtatg
     3901 gacacgggct ctcccgggcc tggaacccca ggaatccatc gaccgcttct acacgcgccc
     3961 gggcggcggc gtgatcgcga tcggcccggg gctcggcgac gataatccgt ccggggccgt
     4021 gacgatcgcc ttcgaccccg agggcgcgct gctgtgggtc gatcgccagg cacaccccga
     4081 gctcagcgac gtgcgcaaca acgccgccgt gatcgtcgac gggaccgtca tggtctccgg
     4141 ctgcggcctc ggcccgccga gctccgacac cacggcatgg atgctgaagc tcgcgctctg
     4201 accgccgcgc gagcccgccg gccg
//
LOCUS       DASWNJ010001818         5390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_343719, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001818 DASWNJ010000000
VERSION     DASWNJ010001818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5390
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343719"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2961)
                     /locus_tag="VGB85_30460"
     CDS             complement(<1..2961)
                     /locus_tag="VGB85_30460"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0138462.1"
                     /translation="MSDLSHETEEATPSATPPQPVIAGRVGLSDLALDRPVMVGMILL
                     AIFVLGAIAVRALPLAFLPASGSARVTVRLDLARTSPELVEREVIRPVEQQMAGLRGL
                     QKIQVGSGGWGVRFNLEFSPGSDIAARKAEIRERLDRIRPTLPDTISRISLDSWGRSD
                     EPVFELRLASETGLTRDYELLQRLILRPIERIPGVSRIELEGATPRELEVALDLEAVQ
                     RAGADLGQISGVLRGARQGRSLGVLRQADINPGVRSPAVAADPEAFAALPIPRRAGAG
                     ATPRGPPATGLSSGTATTTASPASIRAAGMAAPSVQNLAAGAMAPRSATALSSAAWST
                     SATSTTGAGAAAARLGEVADVEIHPRENRNFRRLDGQLGVDLEVYAEAGASPVEVAQS
                     VRRLLAELQASPRLGDLQMIVFHDQGEIILKTLADLRNTGIYGGLLGVVVLYLFLRRI
                     GTTLVAALCIPLTLLATCGVLLMRGEELNCIVLLGLVLGVGMLVDNAVVIVEAIEHHA
                     ARGVPALQAIRQGGRDVSLATVASTLSSVIVFLPLVFGDPADPMAALLSPLGLTFAIV
                     LLCSLFISQTLVPLLMPAVLRMNRNHAPHRTPVMDRIGAGYRWLIARTLRHRRVTLAL
                     SVVLAATAYYPATTLNYNMTELEPKPEQLEIGLQFVGSPGYREIGGKVEAIEAALLAR
                     KDELAIAHVSCRYSDFWARCGAHPLVRAESEAEVEAFTEAVTRALPEQPGVRYLIGEN
                     AQSQWRGTGDRREVELAIKGEDMGVLMQLGEQAAEHLRKTLPRGDSSNIEAGGYDAVI
                     GPYDEGSRELHVKLDTAAMHRFGLRADDVARLVQTAFQGLPLGQVRGPDGEIELRLSA
                     DGGPTRAGENGEADDGPSLADLRDLKIPVTTAAGPGEVAVGSLASFERTRSPFFVQRV
                     DRETQVKVKVRFFTADPQKNRKVVDEAMKQFNFPPGYSSGDWARWRSRDGSQRDTLIN
                     LGLCLLLVYAVMASL"
     gene            complement(2963..>5390)
                     /locus_tag="VGB85_30465"
     CDS             complement(2963..>5390)
                     /locus_tag="VGB85_30465"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0138463.1"
                     /translation="TVRRAPIARTELALVSGQDAVELAVYREGDANTVAVAAGVLEAL
                     PNLRLMPGEQVVVLSNQSSFIESAIAEVQENTLLGGALAVLVLLFFLRDLRATAVIAV
                     SIPISLLATFVPLQALGVSLNLMSLGGLALGVGKLVDNSIVVLESIARVRERAAASGL
                     SEGTGLAATAVRGTVEVASSVVASTLTTVAVFLPMAFVEGVAGHLVRDLSYTVSFSIL
                     SSMVVSLTLVPVLQSLGEAARASDPPPTGSAPLAWLVVIPALLAWPLRFLVRMLGRLL
                     AVIATPLWIAYDWLERLYPRLLRLALRARVLVLLAALGLTIASVQVAAGLGRTLLPEL
                     QQGEFFVQLGLPQGSSLDRSAELAADVAGAVQDDPRVAQVFARVGSMTAGDSATGTVQ
                     GTHLAQVNVRLGALAPEDTAAAEADLFARMQGAVREPEATTRLGRPALFSFDAPIEVQ
                     VFSDEPGRAIEHARRLLPDLQQVAGLADVVPDDLAGRPEVRVDFDRERLSRLGLGVDV
                     AALSVQRVIQGEVATKRHAAARQLDVRVQLPRVDRTRSEDVARVQVAVQIGVPVLLSA
                     VAELTPAVGPAEIRHIEGRRGLRIRARLAGTDLGRVAEDVQAVLARHASDDPRVEAVV
                     SGQAREMGASLDSLLFAAVIAVFLIYVVMASSFESLHHPLLIMGTVPLALIGVSAACV
                     MTGTPISAMVGIGAIILGGIVVNNAIVLVNTVNYGRGQGLSVDEALIQAGSLRLRPIL
                     MTTATTVLGLLPMAIGLGDGAALRQPLAVTVIGGLVASTALTLVVIPCAYSLVPGRRR
                     AAWAELTGEG"
BASE COUNT          775 a         2000 c         1930 g          685 t
ORIGIN      
        1 cagcgaggcc atcaccgcgt agacgagcag caggcacagg ccgaggttga tcagcgtgtc
       61 gcgctgcgag ccgtcgcggc tgcgccagcg ggcccagtcg ccgctgctgt agccgggcgg
      121 aaagttgaac tgcttcatcg cctcgtcgac gaccttgcgg ttcttctgcg ggtcggcggt
      181 gaagaagcgg accttgacct tgacctgcgt ctcacggtcg acgcgctgga cgaagaacgg
      241 gctgcgcgtg cgctcgaagc tcgccagtga gccgaccgcg acctcccccg ggcccgccgc
      301 ggtcgtcacc gggatcttga ggtcgcgcag gtcggcgagg ctcgggccgt cgtccgcctc
      361 gccgttctca ccggcgcggg tcggtccgcc gtcggccgac aggcgcagct cgatctcacc
      421 gtcgggcccg cggacctgcc cgagcggcag gccctggaag gccgtctgga ccaggcgggc
      481 gacgtcgtcg gcgcgcaggc cgaagcggtg catcgccgcg gtgtcgagct tgacgtgcag
      541 ctcgcgcgag ccctcgtcgt agggtccgat cacggcgtcg taaccaccgg cctcgatgtt
      601 gctcgagtcg ccgcgcggga gcgtctttcg caggtgctcg gccgcctgtt cgccgagctg
      661 catcaacacg cccatgtcct cgcccttgat cgccagctcg acctcgcggc ggtcgccggt
      721 gccgcgccac tggctctggg cgttctcgcc gatcaggtag cgcacgcccg gctgctcggg
      781 cagcgcccgc gtcacggcct ccgtgaacgc ctcgacctcc gcctccgact cggcgcggac
      841 caaagggtgc gcgccgcagc gggcccagaa gtcactgtag cggcagctga cgtgcgcgat
      901 cgccagctcg tccttgcggg ccagcagcgc cgcctcgatc gcctcgacct tgccgccgat
      961 ctcgcggtag ccggggctgc cgacgaattg cagcccgatc tcgagctgct cgggcttggg
     1021 ctcgagctcc gtcatgttgt agttgagcgt cgtcgccggg taataggcgg tcgccgcgag
     1081 cacgacactg agcgcgagcg tcacccggcg gtggcgcagc gtgcgggcga tcaaccaccg
     1141 gtagccggcg ccgatgcggt ccatcaccgg cgtgcggtgc ggcgcgtggt tccggttcat
     1201 gcgcagcacc gcgggcatca gcagcggcac cagcgtctga ctgatgaaca ggctgcacag
     1261 cagcacgatc gcaaaggtca gcccgagcgg ggacagcagc gccgccatcg ggtcggccgg
     1321 gtcgccgaac accagcggca ggaacacgat cacgctcgac agcgtcgacg cgacggtcgc
     1381 caggctgacg tcgcggccgc cttgccggat cgcctgcagc gcgggcacgc cgcgggccgc
     1441 gtggtgctcg atcgcctcga cgatgacgac cgcgttgtcg acgagcatgc cgaccccgag
     1501 caccagcccg agcaggacga tgcagttgag ctcctcgccg cgcatcagca gcaccccgca
     1561 ggtcgccagc agcgtcagcg ggatgcacag cgccgcgacc agcgtggtgc cgatccggcg
     1621 caggaacagg tacagcacga ccacgccgag caggccgccg tagatgccgg tgttgcgcag
     1681 gtccgcgagg gtcttgagga tgatctcgcc ctggtcatga aacacgatca tctgcaggtc
     1741 gccgaggcgc gggctcgcct gcagctcggc gagcagccta cgcaccgact gggcgacctc
     1801 gaccgggctc gcgccggcct ccgcgtacac ctcgaggtcg accccgagct ggccgtcgag
     1861 gcggcgaaag ttgcggttct ctcgcgggtg gatctcgacg tcggcgacct cgccgaggcg
     1921 cgccgccgcg gccccggccc cggtcgtgct cgtcgccgac gtcgaccacg ccgcgctgct
     1981 cagcgccgtc gccgagcgcg gcgccatcgc cccggccgcg aggttctgca ccgagggcgc
     2041 cgccatgccg gccgcgcgga tgctcgccgg actcgccgtg gtcgtggctg tccccgagga
     2101 caggccggtc gccggcggtc cgcggggcgt cgcgccagcc ccggcgcggc gcgggatcgg
     2161 cagcgccgcg aatgcctcgg ggtcggccgc gaccgccggg ctgcgcacgc cggggttgat
     2221 gtccgcctgg cgcagcaccc cgagcgagcg accctgtcgc gcgccgcgga gcaccccact
     2281 aatttggccg aggtcggcgc cggcgcgctg gaccgcctcg aggtcgagcg ccacctcgag
     2341 ctcgcgcggc gtggcgccct cgagctcgat ccgcgacacc cccgggatgc gctcgatcgg
     2401 gcgcaggatc agccgctgca gcagctcata gtctcttgtc aggccggtct cgctggcgag
     2461 gcgcagctcg aacaccggct cgtcgctgcg gccccacgag tcgagcgaga tccggctgat
     2521 cgtgtccggc agcgtcggcc ggatccggtc gaggcgctcg cggatctcgg ccttgcgcgc
     2581 cgcgatgtcg gatcccgggc tgaactcgag gttgaagcgc acgccccagc cgccgctgcc
     2641 gacctgaatt ttctggagtc cgcgcagccc ggccatctgc tgctcgaccg ggcggatgac
     2701 ctcgcgctcg accagctcgg ggctggtgcg cgcgaggtcg aggcgcacgg tgacgcgggc
     2761 cgagccgctc gccggcagga acgccagcgg cagcgcgcgc acggcgatcg caccgagcac
     2821 gaagatcgcc agcaagatca tgccgaccat caccgggcgg tcgagcgcga ggtccgagag
     2881 cccgacccgc ccggcgatca ccggctgcgg cggcgtcgcg ctcggcgtcg cctcctctgt
     2941 ctcatgggac aggtcggaca cgtcagccct cccccgtcag ctcggcccac gcggcgcggc
     3001 ggcggccggg gaccaggctg taggcgcagg ggatcacgac cagcgtcagc gcggtcgagg
     3061 cgaccaggcc gccgatcacc gtcaccgcga gcggctggcg cagcgcggcg ccgtcgccga
     3121 ggccgatcgc catcggcagc agcccgagca cggtcgtcgc ggtcgtcatc aagatcggcc
     3181 gcaggcgcag gctgccggcc tggatcagcg cctcgtcgac cgacaggccc tgcccgcggc
     3241 cgtagttgac ggtgttgacg agcacgatcg cgttgttgac gacgatgccg ccgaggatga
     3301 tagctccgat gccgaccatc gccgagatcg gcgtgccggt catcacgcac gccgcgctga
     3361 cgccgatcag cgccagcggc acggtgccca tgatcagcag cgggtggtgc aggctctcga
     3421 agctcgacgc catcaccacg tagatcagga acaccgcgat caccgcggcg aacagcaggc
     3481 tgtcgagcga ggcgcccatc tcgcgggcct ggccgctgac gaccgcctcg acgcgcgggt
     3541 cgtcgctcgc gtggcgggcg agcaccgcct gcacatcctc ggcgacgcgc ccgaggtcgg
     3601 tgccggccag gcgcgcgcgg atccgcaggc cgcggcggcc ctcgatgtgc cggatctccg
     3661 cggggccgac cgccggggtc agctcggcga ccgccgaaag cagcaccggc acgccgattt
     3721 gcacggcgac ctgcacgcgc gcgacgtcct cgctgcgcgt acggtcgacc cgcggcagct
     3781 gcacgcggac gtcgagctgg cgagcggccg cgtgcctctt ggtcgcgacc tcgccctgga
     3841 tcacccgctg caccgacagc gccgcgacgt cgacgccgag gccgaggcga ctcaggcgct
     3901 cgcggtcgaa gtcgacgcgc acctcggggc ggccggcgag gtcgtcgggc acgacgtcgg
     3961 cgagcccggc gacctgctgc aggtccggca acaggcgccg cgcgtgctcg atcgcccgcc
     4021 cgggttcgtc gctgaacacc tggacctcga tcggcgcgtc gaacgagaac agcgccggtc
     4081 gaccgagtcg cgtcgtcgcc tccggctcgc gcaccgcccc ttgcatgcgc gcgaacagat
     4141 cggcctccgc ggcggcggtg tcctccggcg ccagcgcgcc gaggcgcacg ttgacctgcg
     4201 cgaggtgggt gccctgcacc gtcccggtcg cgctgtcacc cgcggtcatg ctgccgacgc
     4261 gcgcgaacac ctgggcgacg cgcgggtcgt cctgcaccgc gccggcgacg tcggccgcga
     4321 gctcggcgct gcggtcgagg ctgctgccct gtgggagccc gagttgcacg aagaactcgc
     4381 cctgttgcag ctccggcagc agcgtgcgcc cgagcccggc ggcgacctgg acgctggcga
     4441 tcgtcaggcc gagcgcggcg agcagcacga gcacgcgcgc gcgcagggcc agccgcagca
     4501 gccgcgggta cagccgctcg agccagtcgt aggcgatcca cagcggcgtg gcgatcaccg
     4561 cgagcaggcg gccgagcatc cggaccagga agcgcagcgg ccaggcgagc agcgccggga
     4621 tgacgaccag ccacgccagc ggcgcgctcc cggtcggcgg ggggtcgctc gcgcgcgcgg
     4681 cctcgccgag gctctgcagc accggcacca gcgtcagcga cacgaccatc gacgacagga
     4741 tgctaaacga caccgtgtag ctgaggtcgc gcaccaggtg cccggcgacg ccctcgacga
     4801 aggccatcgg caggaacacc gcgacggtcg tcagcgtcga ggcgaccacc gaggacgcga
     4861 cctcgacggt gccgcgcacc gcggtcgccg cgagacctgt cccttcggac agccccgacg
     4921 cggccgcgcg ctcgcgcacg cgggcgatcg actcgagcac gacgatgctg ttgtcgacga
     4981 gcttgccgac gccgagcgcg aggccgccga ggctcatcag attgagcgac acgcccagcg
     5041 cctgcagcgg cacgaaggtc gcaagcagcg agatcgggat cgacaccgcg atcacggcgg
     5101 tcgcccgaag atctctcagg aagaacaaaa gcaccagcac cgcgagcgcc ccgccgagca
     5161 gggtgttctc ctgcacctcg gcgatcgccg actcgatgaa gctgctttga ttactgagca
     5221 ccacgacctg ctcgcccggc atcaggcgca ggttcggcag cgcttccagc accccggccg
     5281 cgacggcgac ggtgttggcg tcaccctcgc gatacaccgc gagctcgacc gcgtcctgcc
     5341 cggacaccag cgcgagctcg gtgcgcgcga tcggggcccg gcgcaccgtg
//
LOCUS       DASWNJ010001819         1769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_415143, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001819 DASWNJ010000000
VERSION     DASWNJ010001819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415143"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1186)
                     /locus_tag="VGB85_30470"
     CDS             complement(<1..1186)
                     /locus_tag="VGB85_30470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0138464.1"
                     /translation="MSDSANTGEYNQQTTSLWMHTADVPIYAPLTEDIAAEVCIVGGG
                     IAGLTTAYLLAVEGRSVVVLERGPVGGVNTARTTAHLSNVIDDRFLYLERVRGTNMAK
                     LAYESHGMAIEQIATIVQDEHIECDFERLPGYLFLAPHHTQKEIDDELEAAKRAGFTE
                     AEKLPDAPVAGLGPCLHFPRQGQFHALKYVAGLCRAITRLGGRIFAHSPAAEIKGGEQ
                     PSVRTTAEHTVTAGSVVVATNSPITDRVAMHTKQAPYTTYVVGLRVEPGTIPHGLYWD
                     TQDPYHYVRLQWVAGTDGGPPHEVLIVGGEDHKTGQAQDGQRRFEELERWSRENFPGL
                     GEVVERWSGMVQETIDGVGFIGKDPGGGENVYVATGDSGQGMTHGTIAGMLLRDLILK
                     RDN"
     gene            1368..>1769
                     /locus_tag="VGB85_30475"
     CDS             1368..>1769
                     /locus_tag="VGB85_30475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0138465.1"
                     /translation="MPAPPLRATRLRKRYGATTLFDGLDLTVAPGEVVALLGPNGTGK
                     STLIGCVTGAVITDEGEVEIGGHDLMRAPLAARAALRTLPQEVEVPEGLTGRELLGFH
                     ASVFGERHGLDAALALADLGPAIEALATTYSV"
BASE COUNT          233 a          640 c          627 g          269 t
ORIGIN      
        1 ggttgtcgcg cttgaggatc aggtcgcgca gcagcatgcc ggcgatcgtg ccgtgggtca
       61 tgccctgacc cgagtcaccc gtcgccacgt agacgttctc gccgccgccc gggtccttgc
      121 cgatgaagcc gacgccgtcg atcgtctcct ggaccatgcc gctccagcgc tcgacgacct
      181 cgccgagccc cggaaagttc tcgcgcgacc acctctcgag ctcctcgaag cggcgctggc
      241 cgtcctgcgc ctgaccggtc ttgtggtcct caccgccgac gatcagcacc tcatgcggcg
      301 ggccaccgtc ggtgccggcg acccactgca gacgcacgta gtggtacggg tcctgggtgt
      361 cccagtacag cccgtggggg atcgtgccgg gctcgacgcg caggccgacc acgtaggtgg
      421 tgtacggcgc ctgcttggtg tgcatcgcga cgcgatcggt gatcggcgaa ttggtggcga
      481 cgacgaccga gcccgcggtg acggtgtgct cggcggtggt gcgcacgctc ggctgctcgc
      541 cgcccttgat ctcggccgca gggctgtgcg cgaagatccg cccgccgagc cgggtgatcg
      601 cgcggcacag gccggcgacg tacttgagcg cgtggaactg gccctgccgc ggaaaatgca
      661 ggcacggccc gagcccggcg accggcgcgt ccggcagctt ctcggcctcg gtgaagccgg
      721 cccgcttcgc ggcctcgagc tcgtcgtcga tctccttttg cgtgtgatgc ggggcgagga
      781 acaggtagcc cggcaaccgc tcgaagtcac actcgatgtg ctcgtcctgg acgatcgtgg
      841 cgatctgctc gatcgccatg ccgtggctct cgtacgcgag cttcgccatg ttcgtgccgc
      901 gcacgcgctc gaggtagagg aagcggtcgt cgatcacgtt cgagaggtgg gcggtggtcc
      961 gcgccgtgtt gacgccgccg acgggcccac gctcgagcac gacgaccgaa cgaccctcga
     1021 ccgccagcaa gtaggcggtc gtcaggcccg cgatcccgcc gccgacgatg cacacctcgg
     1081 cggcgatgtc ctcggtcagg ggcgcgtaga tcggcacgtc cgcggtgtgc atccacaacg
     1141 aggtcgtctg ctggttgtac tcgcccgtgt tcgcggagtc cgacatgccg ggtctttggc
     1201 ccaagtcgcc gcgcggtcaa gcgcagacgc gcgggtcacc ccggacgccg cgcgcggacc
     1261 gtccacatgc ggtcccgcga cctgcgcgcg aggcccgcag ggtcgcagga ccccgtcccc
     1321 ggggtcctgc gcgcgggagc cccacgccgc gcgatagtcc gccgcctgtg cccgcaccgc
     1381 cgctccgcgc gacccgccta cgcaagcgct acggcgcgac gacgctgttc gacggcctcg
     1441 atctgacggt cgcgcccggc gaggtggtcg cgctgctcgg ccccaacggc accggcaagt
     1501 cgacgctgat cggctgcgtc accggggccg tgatcaccga cgagggcgag gtcgagatcg
     1561 gcgggcacga cctgatgcgc gcgccgctgg ccgcacgcgc ggccctgcgg acgctgccgc
     1621 aggaggtcga ggtccccgag ggcctcaccg gccgcgagct gctggggttt catgcatcgg
     1681 tgttcggcga gcgtcacggc ctcgacgccg cgctcgcgct cgccgacctc gggccggcga
     1741 tcgaggcgct ggcgaccacg tacagcgtc
//
LOCUS       DASWNJ010001820         2768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_103678, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001820 DASWNJ010000000
VERSION     DASWNJ010001820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103678"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1588)
                     /locus_tag="VGB85_30480"
     CDS             complement(<1..1588)
                     /locus_tag="VGB85_30480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138466.1"
                     /translation="MAFGIGASVGAGLVVAAGLVAASPAPLRLGASAEPLLVVPTPGP
                     LGGLWDALWLALPVGERGARGDLAVLLAILAVAAAAGVLAFRLYRGTGRGAAAFATVP
                     VCAALLLGWAHEDVAAPAPLGFTLLATLGLAAVIDGRVRGRGPGPATGLRGATATIAA
                     ALLWPRVGPAWAAVVLTYMAWSAGRLGWRGISVVAVIAIGLAYVTWPAGQDLRLVMDL
                     GPVSRSTGPALLDLALALAVLLVAPLRWRGGGLLLALVLAGLLLGDRSGPVVPMPALL
                     VLLAVAASGWIWLAGSVRRRWVARLGVVVATMAILGLVAAGFVPVAEPVAARRPEASL
                     LALHQRGLLAPGDVLLAHDPWLAAAFAAAQRDEGVRPDVELHAVASVDPARLSERLAG
                     WARAGRRVLSDSFNYAGRWQAAWALDSGPLFWFIGTAGAGDRDFTDLREHSPGPALAT
                     LAPAERARWERLHIERARHRRALGHFDEALLALPFDDDTLAALAQRLQLAKLSRLPAI
                     SGSELGAGAWPSAPPPASALA"
     gene            1797..>2768
                     /gene="tig"
                     /locus_tag="VGB85_30485"
     CDS             1797..>2768
                     /gene="tig"
                     /locus_tag="VGB85_30485"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEY0138467.1"
                     /translation="METQLEKINAVECRVRVQIPWEEISPRFDKKLRELQGSARLPGF
                     RPGKVPPHMVERLFGKNVRDELARDIVQETFQSAVSKHEKVPLTQPVLEEGTLTKGQT
                     FTYAARFEVPPEIQPKDYTGVPVRRRPSVVDESKVDAQIKAKQDELTELRAIAEGTER
                     PTHAGDTWTLDIDGKLGDVNVSRKDVRLEIGDASEYLPGLSKALEGTPLSAVGSTRTI
                     TFLPTQERIRADLRGKEAVLTIGFRDVREKHVPNLDDEFARDSGEADSLEALRAKYAE
                     QVREEDKNEAERDARKRLVEALLERNNFEVAPSLVAREVAAQVDLFKR"
BASE COUNT          439 a         1110 c          939 g          280 t
ORIGIN      
        1 ccgccagcgc gctggcgggc ggcggcgccg agggccacgc cccggccccg agctcgctgc
       61 cggagatcgc aggcaggcgg ctcagcttgg ccagctgcag gcgctgcgcg agcgcggcga
      121 gcgtgtcgtc gtcgaagggc agggcgagca gcgcctcgtc gaagtgcccg agcgcccgcc
      181 ggtgccgcgc ccgctcgata tgtaagcgct cccagcgggc gcgctccgcc ggcgccagag
      241 tcgcgagcgc cggccccggg ctgtgctcgc gcaggtcggt gaagtcgcgg tcccccgcgc
      301 ccgccgtgcc gatgaaccag aacagcggcc cgctgtcgag cgcccacgcg gcctgccagc
      361 ggccggcata attaaaactg tccgaaagga cacgtcggcc cgcgcgcgcc cagccggcca
      421 ggcgctcgct gagccgcgcc gggtcgacgc tggcgaccgc gtgcagctcg acatccggcc
      481 ggacgccctc gtcgcgctgg gccgccgcga acgcggccgc gagccacggg tcatgggcga
      541 gcagcacgtc gccgggcgcg agcagcccgc gctggtgcag cgccagcaac gacgcctccg
      601 gacgccgcgc cgccaccggc tccgccaccg gcacgaagcc tgcggcgacg agcccgagga
      661 tcgccatcgt tgcgaccacc accccgaggc gcgcgaccca gcgccggcgc acgctgccgg
      721 ccagccagat ccacccgctc gcggccaccg cgagcagcac cagcagcgcc ggcatcggca
      781 ccacgggccc gctgcgatcg ccgagcaaca gccccgcgag caccagcgcg agcagcagcc
      841 cgcctccacg ccagcgcagc ggcgcgacca gcagcaccgc cagcgcgagc gccaggtcga
      901 gcagggccgg cccggtcgac cgcgacaccg gccccagatc catcaccagg cgcagatcct
      961 gtcccgcagg ccaggtcaca tacgcgagcc cgatcgcgat caccgcgacg acgctgatgc
     1021 cacgccagcc caggcgcccc gcgctccacg ccatgtacgt gagcaccacc gccgcccacg
     1081 ccggcccgac gcgcggccac agcagcgccg ccgcgatcgt cgctgtcgcc ccgcgcagcc
     1141 ccgtcgcggg tccgggcccg cggccgcgta cccgcccatc gatcaccgct gccagtccga
     1201 gcgtcgccag cagcgtgaag ccgaggggcg ccggtgcggc cacgtcctca tgcgcccatc
     1261 ccagcaacag cgccgcgcac accgggaccg tcgcgaacgc ggccgcaccg cgacctgtcc
     1321 cgcggtacag gcgaaatgcc agcaccccgg cggccgccgc caccgcgagg atcgccagca
     1381 gcaccgccaa gtcgccccgg gctccgcgct cgccgaccgg cagcgcgagc cacagcgcgt
     1441 cccacaagcc gcccagcggc cccggcgtcg gcaccacgag caggggctcc gcgctcgcgc
     1501 ccaggcgcag cggcgccggc gaggccgcga ccaggccggc cgcgaccacc agccccgcgc
     1561 cgaccgatgc accgatcccg aaggccacgc ctggcgatcc cggctccgcg gtatccccgg
     1621 cgggcgacga gtgcgggtta tcacggcctc cagcgggtgc gagccccgta cggctggcgg
     1681 tcgcgcgcac gtcgaggcca gcatagcagg ggctcgcgga ccgcgtccgg cgcggcgggg
     1741 cttgtgtcgc ctccgcccgt ctgttacgaa gccagccccg aaaccgggtc tttgccatgg
     1801 aaactcagct cgagaagatc aatgccgtcg agtgtcgcgt gcgtgtccag atcccgtggg
     1861 aggagatctc accgcgcttc gacaagaagc tgcgcgagct tcagggctcg gcgcgtctgc
     1921 ccggctttcg ccccggcaag gtcccgccgc acatggtcga gcgtctgttc ggcaagaacg
     1981 tccgcgacga gctcgcgcgt gacatcgtgc aggagacctt ccagtcggcg gtgagcaagc
     2041 acgagaaggt gccgctcacc cagccggtgc tcgaggaggg caccctgacg aagggccaga
     2101 ccttcacgta cgcggcccgc ttcgaggttc cgccggagat ccagcccaag gactacaccg
     2161 gcgtgccggt ccgtcgccgc ccctccgtcg tcgacgagtc caaggtcgac gcgcagatca
     2221 aggccaagca ggacgagctg accgagctgc gggcgatcgc cgagggcacc gagcggccga
     2281 cccacgccgg cgacacctgg accctcgaca tcgacggcaa gctcggcgac gtcaacgtct
     2341 cgcgcaagga tgtccgcctc gagatcggcg acgccagcga gtacctcccg ggcctgagca
     2401 aggcgctcga gggcacccca ttgtctgcag tcggctcgac ccgcacgatc acgttcctgc
     2461 cgacgcagga gcgcatccga gccgacctgc gcggcaagga ggccgtcctc acgatcggct
     2521 tccgcgatgt ccgcgagaag cacgtcccca acctcgacga cgagttcgcc cgcgactccg
     2581 gcgaggccga ctcgctcgag gccctgcgcg ccaagtatgc cgagcaggtc cgcgaggagg
     2641 acaagaacga ggccgaacgc gacgcccgca agcgcctcgt cgaggccctg ctcgagcgca
     2701 acaacttcga ggtcgcgccc tcgctggtcg cccgcgaggt cgccgcccag gtcgacctgt
     2761 tcaagcgc
//
LOCUS       DASWNJ010001821         1805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_103688, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001821 DASWNJ010000000
VERSION     DASWNJ010001821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103688"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VGB85_30490"
     CDS             complement(<1..559)
                     /locus_tag="VGB85_30490"
                     /inference="COORDINATES: protein motif:HMM:NF013768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEY0138468.1"
                     /translation="MVELNARSREVFRQIVDAYVATGEPVGSRTIARRLEDQLSAATI
                     RNVMADLEELGLLRAPHTSAGRLPTEAGLRFYVDGLLEIGSLSDEERRTIEAQCAGRG
                     RSIEAVLGDASDALSGLTRYAGLVTAPMVDRPFKHIEFVPLGPTRALVVLVSDNGLVE
                     NRVVEVPIGMPASTLVEAGNYLTARL"
     gene            676..1779
                     /locus_tag="VGB85_30495"
     CDS             676..1779
                     /locus_tag="VGB85_30495"
                     /EC_number="4.2.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF043002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:1901605 - alpha-amino acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cis-3-hydroxy-L-proline dehydratase"
                     /protein_id="HEY0138469.1"
                     /translation="MRITRIWAYRVLLPLVEGRYSWSLGKSVDVFDSTLVRVATDDGL
                     EGWGETCPLGPVYLPAYAEGVRAGIAKVGPAVIGLDPREHGIVNSAMDTALKGHPYAK
                     AALDIACWDLAGKAAGVPVATLLGGREGEAVELYRAISQDTPERMAGRVAGYRAEGYR
                     RFQLKSGSSVADDVARIRAVREVMAEGEILVADANTGWLPHEAVRVVNAVRDLDVYVE
                     QPCATYEECLTVRRQTPHPFVLDEVIHDVPSLLRAAADGAMDVVNLKISKVGGLTRAR
                     LMRDLCVELGIAMTIEDSWGGDVTTAAIAHLAHSTPERYRFSATDFNSYVSRRTAEGA
                     PARVEGRMRAADRPGLGVEPLRGELGPPVIEIA"
BASE COUNT          259 a          640 c          670 g          236 t
ORIGIN      
        1 cgagccgcgc ggtaagatag ttgccggcct ccaccagggt cgaggccggc atgccgatcg
       61 gcacctcgac cacgcggttc tcgaccagcc cgttgtcgct gaccaggacc acgagcgccc
      121 gggtcggccc gagcggcacg aactcgatgt gcttgaacgg ccggtcgacc atgggtgccg
      181 tgaccagacc ggcgtagcgg gtgagcccgg agagcgcgtc cgacgcgtcg ccgagcacgg
      241 cctcgatgct ccgcccgcgg ccggcgcact gggcctcgat cgtccggcgc tcctcgtccg
      301 acaggctgcc gatctcgagc agcccgtcga cgtagaagcg caggcccgcc tcggtcggca
      361 ggcggcccgc cgaggtgtgg ggggcccgca gtaggcccag ctcctcgagg tccgccatca
      421 cgttgcggat ggtcgccgcg gagagctggt cctccaggcg ccgcgcgatc gtgcgcgagc
      481 ccaccggctc gccggtggcg acgtaggcgt cgacgatctg acggaagacc tcgcgcgagc
      541 gggcgttaag ttcgacgatc ctcgcacgcg caagagcgga gcatgaggac tgccgatgat
      601 gtaggaaccg gcctgctgcc cgtcaaccga ggccggccgc cgccgcgcgg tcgccgcagc
      661 cggagagttg gtgcgatgcg catcacgcgg atctgggcct accgggtcct gctgccgctg
      721 gtcgagggcc gttacagctg gtcgctcggc aagtcggtcg acgtcttcga cagcaccctc
      781 gtacgggtgg cgaccgacga cggcctggag ggctggggcg agacctgccc gctcgggccg
      841 gtctacctgc ccgcctacgc cgagggcgta agggccggga tcgcgaaggt cgggcccgcg
      901 gtgatcggcc tcgatcccag ggagcacggc atcgtcaact ccgccatgga cacggcgctc
      961 aagggccacc cctacgccaa ggcggcgctg gacatcgcgt gctgggacct cgccggcaag
     1021 gcggcgggcg tgccggtggc gaccctcctg ggcgggcgtg agggcgaggc cgtcgagctc
     1081 taccgggcga tctcgcagga cactccggag cgcatggccg ggcgcgtggc cggctaccgc
     1141 gccgagggct accgccgctt ccagctcaag tccggctcgt cggtcgccga cgacgtcgcg
     1201 cgcatccgcg ccgtccgcga ggtcatggcc gagggcgaga tcctggtcgc cgacgcgaac
     1261 accggctggc tgccgcacga ggcggtccgg gtggtcaacg ccgtgcgcga cctcgacgtc
     1321 tacgtcgagc agccctgcgc cacctacgag gagtgcctga ccgtgcgccg gcagacgccg
     1381 cacccgttcg tgctcgacga ggtgatccac gacgtgccct cgctgctgcg cgcggccgcc
     1441 gacggtgcca tggacgtcgt caacctgaag atctcgaagg tgggcggcct gacccgcgcc
     1501 cgtctgatgc gcgacctgtg cgtcgagctg gggatcgcga tgacgatcga ggacagctgg
     1561 ggcggcgacg tgacgaccgc cgcgatcgcg cacttggcgc acagcacgcc ggagcggtac
     1621 cgcttctccg cgaccgactt caacagctac gtgagccgcc ggaccgcgga gggtgcgcca
     1681 gccagggtcg aggggcggat gcgcgccgcc gaccgaccgg ggctcggcgt cgagccgctg
     1741 cgcggcgaac tcggcccgcc ggtcatcgag atagcctgag gagatgccgg gacgggcgcc
     1801 cacgc
//
LOCUS       DASWNJ010001822         2314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_384234, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001822 DASWNJ010000000
VERSION     DASWNJ010001822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384234"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1791
                     /locus_tag="VGB85_30500"
     CDS             <1..1791
                     /locus_tag="VGB85_30500"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0138470.1"
                     /translation="NVPWADDNTAGARFEDQARALRGLEHPNIAAIIDFGREPGRVFV
                     IGEYVEGKLLSTYIEQLGRLTLEGFVPIAAQILKGLGVTHARGLVHRDLKPANIVLVE
                     EQGRANYVKILDLGLAQLLEGPARGDDVPPVGTPDFLAPEQILNKPVDPRTDVYALGV
                     VFYQMLSGRLPFVADGQGGARSVLYKHVNDKPIPLTEVLPAGHNLPDGLIELVHDCLA
                     KNPDNRPADANEVVERLIDSVPAAMFRLPVAGATIPTIPRRVTGEITRHQTGDLPRSQ
                     TGDFGRIPAYRPEPPPPVLAQHDRTLAAAVEASGFRPVATIDPALTSGDTRQGEIPRS
                     GGAGWVLGLLALALTGGLVYLYIQSQQETATQVAPAVAAPPADAAVEQAIARARALEA
                     AGKTQEAIGAYEAILAGDPNHALARERVAALQAIATPPAPDPIPEPAQPEPTPTTPDP
                     IPVSDPTAGAAKTSDPGPDVAADPSPKEVKPDPTPTKPPEPVHVQFTVEAYPKSEIFV
                     DGVTHGKTPQKLDLLSGSHKFEIKAPGFLPHEETIEVAADGQKSLKVKLTRRPAGTGG
                     INRAEEDAASNELDPENDAPTTKIEVPLKR"
     gene            1927..>2314
                     /locus_tag="VGB85_30505"
     CDS             1927..>2314
                     /locus_tag="VGB85_30505"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969317.1"
                     /note="catalyzes the formation of 4-phospho-L-aspartate
                     from L-aspartate and ATP, in Bacillus, lysine sensitive;
                     regulated by response to starvation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEY0138471.1"
                     /translation="MLIVQKYGGSSVGSLERIRNCAARVLATQRAGHEVVVVVSAMSG
                     ETNRLIALANQLAALERGPEADALRGGKGPYVASERAGDRDHERELDQLVCTGENVSA
                     ALLAMALQNAGGAAISLVGPQIGMRTD"
BASE COUNT          367 a          914 c          757 g          276 t
ORIGIN      
        1 aatgtgccct gggcggacga caacaccgcc ggcgcccgct tcgaggatca ggcccgggcg
       61 ctgcgcggcc tcgagcaccc caacatcgcc gcgatcatcg acttcggccg cgagccgggc
      121 cgcgtgttcg tcatcggtga gtacgtcgag ggcaagctgc tctcgaccta catcgagcag
      181 ctcggccgcc tcaccctcga gggcttcgtc ccgatcgccg cgcagatcct caagggcctc
      241 ggcgtcacgc atgcgcgcgg cctggtccac cgcgacctca agcccgcgaa catcgtgctc
      301 gtcgaggagc agggccgcgc caactacgtc aagatcctcg acctcggcct cgcgcagctg
      361 ctcgagggcc ccgcccgcgg cgacgacgtc ccgccggtcg gcacccccga cttcctcgcg
      421 ccggagcaga tcctcaacaa gccggtcgac ccgcgcaccg atgtctacgc gctcggcgtc
      481 gtgttctatc agatgctctc cgggcgcctg cccttcgtcg cggacggcca gggcggcgcg
      541 cgctcggtgc tgtacaagca cgtcaacgac aagccgatcc cgctgaccga ggtgctgccc
      601 gccgggcaca acctcccgga cggcctgatc gagctcgtcc acgactgtct cgccaagaac
      661 cccgacaacc ggcccgcgga cgccaacgag gtcgtcgagc gcctgatcga cagcgtcccg
      721 gcggcgatgt ttcgcctgcc cgtcgccggc gccaccatcc ccaccatccc gcgccgcgtc
      781 accggcgaga tcacgcgcca ccagaccggc gacctgccgc gctcgcagac cggcgacttc
      841 ggccggatcc ccgcgtaccg ccccgagcct ccgccgccgg tgctcgccca gcacgaccgc
      901 accctcgcgg ccgccgtcga ggccagcggc tttcgtccgg tcgccaccat cgaccccgcc
      961 ctgaccagcg gtgacacccg tcagggcgag ataccccgca gcggcggcgc cggctgggtg
     1021 ctcggcctgc tggcgctggc gctgaccggc ggcctcgtct acctgtacat ccagagccag
     1081 caggagaccg cgacccaggt cgccccggcc gtcgcggcgc cgcctgccga cgccgcggtc
     1141 gagcaggcga tcgcccgggc gcgcgcgctc gaggctgccg gcaagaccca ggaggcgatc
     1201 ggcgcctacg aggcgatcct cgccggtgac ccgaaccatg ccctggcccg cgagcgcgtc
     1261 gcggcgctgc aggcgatcgc caccccgccg gcgccggatc cgatccccga gccggcccag
     1321 ccggagccga ccccgacgac ccccgatccg atcccggtct ccgacccgac cgcgggcgcc
     1381 gcaaagacca gcgaccccgg cccggacgtc gccgccgatc cgagcccaaa agaggtcaag
     1441 ccggacccga ccccgaccaa gccgcccgag ccggtgcacg tgcagttcac cgtcgaggcc
     1501 tacccgaagt ccgagatctt cgtcgacggc gtcacccacg gcaagacccc gcaaaagctc
     1561 gacctgctga gcggctcgca caagttcgag atcaaggccc ccggcttttt gccgcacgag
     1621 gagacgatcg aggtcgccgc cgacggccag aagtcgctca aggtcaagct gacgcgcagg
     1681 cccgccggca ccggcggcat caaccgcgcc gaggaggacg ccgcctccaa cgagctcgac
     1741 cccgagaacg acgcgccgac gaccaagatc gaggtcccgc tcaagcgctg accgtctgcc
     1801 tgcgagctgc aacaccagcg cccccgcggt cgaacagctc acctcgggga cgacagccgc
     1861 accgcaattg cggtacagca tcgcctcgcc gggtctgctg ggcccgcaag gacgtgcggt
     1921 caattcatgc tcatcgtgca gaaatacggg gggtcgtcgg tcgggagcct cgagcggatc
     1981 cgcaactgcg cggcgcgggt gctggcgacg cagcgagccg gtcatgaagt cgtcgtcgtc
     2041 gtgtcggcga tgtcggggga gaccaaccgg ctgatcgctc tggccaacca gctcgccgcg
     2101 ctcgagcggg ggccggaggc cgacgcgctg cgcgggggca agggcccgta cgtcgccagc
     2161 gagcgggccg gcgaccgcga ccacgagcgc gagctcgatc agctggtgtg caccggtgag
     2221 aacgtcagcg ccgcgctgct ggcgatggcg ctgcaaaacg ccggcggcgc cgcgatctcg
     2281 ctcgtcggcc cgcagatcgg catgcgcacc gatg
//
LOCUS       DASWNJ010001823         2466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_103710, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001823 DASWNJ010000000
VERSION     DASWNJ010001823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103710"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1563)
                     /locus_tag="VGB85_30510"
     CDS             complement(118..1563)
                     /locus_tag="VGB85_30510"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0138472.1"
                     /translation="MADDANTGLAGTILVIDDERNIRRTLRMVLEGEGATVLDAETAE
                     AGLKLIEQSLIDGDSKSAIEVVITDVLLPGMSGLQLLEKLSQMGDGAPPLPVILISGH
                     ATVSDAVRATRLGAFDFFEKPLARDRVVVSVRNALRHHAAEAELRGLRARARRELIGE
                     SKPMVELQRQITKVGSTRARVLIEGESGTGKELVARAIHEASERREGPFIKVNCAAIP
                     RELIESELFGHERGAFTGATGQKRGLFELANAGTLFLDEIGDMDLNAQAKVLRVLQSG
                     ELMRVGGEKPLKIDVRVLAATHRNLKEQVQSGEFREDLYFRLAVVPIWVPPLRDREGD
                     TALLCRHYLDVACRENGISPKRISPAAQSALDAYAWPGNVRELRNVMERMAILSDGDL
                     ELDDIPLDIRGDSGEDHSSSGGDALVQFGEGAVPPGMSLKGFRDSVERAFILQRLNEH
                     GWNVSKTAEALDVERTHLHKKMKLLGIARGG"
     gene            complement(1671..2351)
                     /locus_tag="VGB85_30515"
     CDS             complement(1671..2351)
                     /locus_tag="VGB85_30515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Yae1 family protein"
                     /protein_id="HEY0138473.1"
                     /translation="MVITPNRRVAAWAARPIDLGPGNEAFCVHVLGPDQIPRISEAHV
                     ARRNPELGFLSALAHADADHDPELVRTALAGLTTLDMDRARSYGSVLLDCIDRALRAA
                     LEASVMEEDNQPRESSVSMEFVDRALEWGRRALIEDERRIAKIEGLEEGRQEGRQEGR
                     QEGLEAGREEGLLLGRREVLRRLLARAGLRLDADDARRIDECCDLATLDRWIDQAIGA
                     SSVAAALA"
BASE COUNT          354 a          922 c          797 g          393 t
ORIGIN      
        1 tcgccgaggc cgacgccgtc atggcccgca cccacctcga cggcaccgag ctcgccggcc
       61 gcacgctgcg cgtcgagcgg gcccagtagg ccatccacat catccatatc atccacatca
      121 tccgccgcgg gcgatgccca gcagcttcat cttcttgtgc aggtgcgtgc gctcgacgtc
      181 gagggcctcg gcggtcttcg agacgttcca gccgtgctcg ttgagccgct gcaaaatgaa
      241 ggcgcgctcg accgagtcgc gaaagccctt gagcgacatg cccggcggca cagcgccctc
      301 gccgaactgg accagcgcgt cgccgccgga gctgctgtgg tcctcgccgc tgtcgccgcg
      361 gatgtcgagc gggatgtcgt cgagctcgag gtcgccgtcc gagaggatcg ccatccgctc
      421 catcacgtta cgcagctcgc ggacgttacc gggccaggcg taggcgtcga gcgccgactg
      481 ggccgccggc gagatccgct tggggctgat gccgttctcg cggcaggcga cgtcgaggta
      541 gtgccggcac agcagcgccg tgtcgccctc gcggtcgcgc agcggcggca cccagatcgg
      601 cacgaccgcg aggcgaaagt acaggtcctc gcgaaactcg ccgctctgga cctgctcctt
      661 gaggttgcgg tgggtcgccg ccagcacgcg cacgtcgatc ttgagcggct tctcgccgcc
      721 gacgcgcatc agctcgccgc tttgcagcac gcgcagcacc ttggcctgcg cgttgaggtc
      781 catgtcgccg atctcgtcga ggaacagggt gccggcgttg gcgagctcga acaggccgcg
      841 cttttgcccg gtcgccccgg tgaaggcgcc gcgctcgtgg ccgaacagct cgctctcgat
      901 cagctcgcgg gggatcgccg cgcagttgac cttgatgaac gggccttccc ggcgctcgct
      961 cgcctcgtgg atcgcacgcg ccaccagctc cttgccggtg ccgctctcgc cctcgatcag
     1021 cacccgcgcc cgcgtgctgc cgaccttggt gatctggcgc tggagctcga ccatcggctt
     1081 cgactcgccg atcagctcgc gtcgcgcccg cgcgcgcaga ccacggagct ccgcctccgc
     1141 cgcgtggtga cgcagcgcgt tgcgcacgct gacgaccacg cggtcgcgcg cgagcggctt
     1201 ctcgaagaag tcgaaggcgc cgagccgggt cgcccgcacc gcgtcggaga ccgtcgcgtg
     1261 cccgctgatc aggatcaccg gcaagggcgg cgcgccgtcg cccatctggc tcaacttctc
     1321 cagcagttgc aggccggaca tgccgggcag cagcacgtcg gttatcacga cctcgatcgc
     1381 cgacttgctg tcgccgtcga tcaacgactg ctcgatcaac ttgagtccgg cctcggccgt
     1441 ctcggcgtcg aggaccgtcg cgccctcgcc ctcgagcacc atccgcaggg tccggcgaat
     1501 gttgcgctcg tcgtcgatga cgaggatcgt gccggcgagc ccggtgttcg cgtcgtccgc
     1561 catgtggccg ccatcctaac cggccgaacc gacgctgtca cccgcgcgcg agcgacccat
     1621 cgtcccatcc ccctgcagtt cagcagcgcc ggacagcaga atcggaaggc tcaggccagc
     1681 gccgccgcga cgctggacgc gccgatcgcc tgatcgatcc agcggtcgag ggtcgcaaga
     1741 tcgcaacatt catcgatccg ccgcgcatcg tccgcgtcca accgcagacc cgcgcgtgcg
     1801 agcaagcgac gcagtacctc gcgccgcccg agcaaaagcc cctcctcgcg tccggcttcc
     1861 agaccctcct gtcggccctc ctgccggccc tcctgccggc cctcctccaa cccctcgatc
     1921 ttggcgatcc gtcgctcgtc ctcgatcaac gccctgcgtc cccactcgag cgcgcggtcg
     1981 acgaactcca tgctgacgga cgactctctg ggctggttgt cttcttccat gacggatgcc
     2041 tccagtgcgg cgcgcagcgc cctgtcgatg cagtcgagga gcaccgaacc atagctccgg
     2101 gcccggtcca tgtccaacgt ggtcaggccc gccagcgccg tgcgcaccag ctccggatcg
     2161 tgatcggcgt cggcgtgggc cagcgccgag aggaagccga gctcggggtt gcggcgcgcg
     2221 acgtgggcct cgctgatgcg agggatctgg tccgggccga gcacgtgcac acaaaacgcc
     2281 tcgttgccgg gtccgaggtc gatcggccgg gccgcccagg ccgccacccg tcgattcggc
     2341 gtgatgacca acaggcaggc ccgcggacat tgtttgcttc gcagcgtcgt cacgtacact
     2401 ggccagctga gcagcttgtc cgggttgatc gtcagctgga cctcgacgcc ggccgccacc
     2461 gtgccg
//
LOCUS       DASWNJ010001824         5577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_526228, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001824 DASWNJ010000000
VERSION     DASWNJ010001824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526228"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VGB85_30520"
     CDS             complement(<1..507)
                     /locus_tag="VGB85_30520"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0138474.1"
                     /translation="MLTPRIPSLSRSGLRPWLFALGCAVTVPTGGCLYETEEPNIKDQ
                     DIRLTFMHTSDIHSRILPYEHNPLYTEEQLGLLPGRGRYGGIARMAYIIKRERAKAGR
                     SLWLDSGDLFQGAPIFNLYRGEPEVRAMTEAGLDAMALGNHEFDWGPVNILDQFAWWA
                     DFPLLAANY"
     gene            complement(674..1528)
                     /locus_tag="VGB85_30525"
     CDS             complement(674..1528)
                     /locus_tag="VGB85_30525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138475.1"
                     /translation="MSAAASQPRPAVRAPGLAPGTVWLLGAALVGGRALLDMTLGSDS
                     AESWQRAVEVLTAAVTVAVALNFSAGDPPRRPWTVLATAMALLPVVRLLSHFQVKFAG
                     IAAAHVLLIVGNVAMAAAVIGFGRVLGSSELLEERSAEDRVRATTFVALLATGALAML
                     AFPTLELVGRGVPATLGAWFAAAAIVVSTLCDALVFAGGMYLVWLVRPLLGGSLARPY
                     LLIAIAGGAFLVVDMLLVTVGATTQTELLGTDLRTFVTKWLGCLAFTGFALAGATQFA
                     LLRSARRR"
     gene            complement(1525..1782)
                     /locus_tag="VGB85_30530"
     CDS             complement(1525..1782)
                     /locus_tag="VGB85_30530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138476.1"
                     /translation="MSIIKPIVPAQFAEHVVEVLTPLLGPHTAKRALAMICKRAGKDP
                     SELGTDDLAIACTTLRPMLRTLIGNDVTVRVLADLGTEVVS"
     gene            1988..3445
                     /gene="guaB"
                     /locus_tag="VGB85_30535"
     CDS             1988..3445
                     /gene="guaB"
                     /locus_tag="VGB85_30535"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY0138477.1"
                     /translation="MATEIKEALTFDDVLLVPRYSEVLPGQVEVGSRLSRGITLNIPL
                     LSAAMDTVTEAQTAICMARHGGLGVVHKNMTPEQQAGEVRRVKKTESGMVVDPVTITA
                     EHTLRQALEIMRACGFSGLPVVQDRKPVGILTSRDLRFETNLDQPVGGVMTRDPITVP
                     QGIALEDARILLHKHRIEKLLVVDPRGELLGLITVKDILNAQSHPNAIKDSAGRLRVG
                     AAVGVGADLMARVQALRSEHVDVIVVDTAHGHSKGVLDAVRGLRGDFPDLQIIAGNIA
                     TAEGYAALCDAGADGVKLGIGPGSICTTRVVAGVGVPQITAILECAAVARKRGVPIIS
                     DGGIKYSGDVVKALAAGADSVMVGSLFAGTDEAPGELVLYQGRSYKVYRGMGSLGAMK
                     AGSKDRYFQGGVSDERKLVPEGIEGRVPYRGPLSDSLYQMVGGLRSGMGYCGARTIVD
                     LQATAELRRITSQGLRESHVHDVIITQEAPNYRRE"
     gene            3451..4998
                     /gene="guaA"
                     /locus_tag="VGB85_30540"
     CDS             3451..4998
                     /gene="guaA"
                     /locus_tag="VGB85_30540"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEY0138478.1"
                     /translation="MAGHQTLIVLDFGSQVTQLIARRVRELGVFAEILPFHTPVEQLR
                     ARDPQAIILSGGPSSLYDEGAPQLDPAVLQLGVPVLGICYGLYLIVAGLGGTVIPATH
                     REFGRADLEIVASEGPLARFGAGESLRVWMSHGDRVERMPAGFRTVGRSPHCEHAAVY
                     DPQRRLWGVQFHPEVTHSDRGQDVLGAFLETAGFRGDWTTAAFIDEAVAKIRARVGEH
                     GTVLCGLSGGVDSSVAALLVERAIGPRLHCIFVDNGLLRKDERAEVEKLFAGRLAQPL
                     VTVDASDRFYGELTSVIDPEQKRKRIGAAFIDVFEAEAQRLGGADFLVQGTLYPDVIE
                     SVNLRGASVTIKTHHNVGGLPERMRMGVVEPLRELFKDEVRKLGAELGLPDDMVWRHP
                     FPGPGLAVRVLGEVTKQRCDLLRDADAIVIEEVRRAGLYRELWQVFAVLLPVQSVGVM
                     GDGRTYENALAIRAVTSTDAMTADWARLPYDLLDVMSRRIINEVRGINRVCYDISSKP
                     PATIEWE"
     gene            5003..>5577
                     /locus_tag="VGB85_30545"
     CDS             5003..>5577
                     /locus_tag="VGB85_30545"
                     /inference="COORDINATES: protein motif:HMM:NF017321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0138479.1"
                     /translation="MRVPHDDGAGPKKPGAKPRAKAAPEIVMPGEKEGAKPAPLERAL
                     VTGVRAQGDAEAPNLRPQRFAEYIGQAALKRKLEVFVRAAKARGEPLDHTLLHGPPGL
                     GKTTMAHILAHELGVKLHLTSGPAIEHKGILAGHLTALEPRDVLFIDEIHRLSPTVEE
                     SLYSAMEDGRIDLPVGEGTRARTIGFELAPFT"
BASE COUNT          866 a         1877 c         2091 g          743 t
ORIGIN      
        1 gtagttggcg gccagcagcg ggaagtcggc ccaccacgcg aactggtcga ggatgttgac
       61 cgggccccag tcgaactcgt ggttgccgag cgccatcgcg tcgaggccgg cctcggtcat
      121 cgcgcggacc tcgggctcgc cgcggtagag gttgaagatc ggcgcgccct ggaacaggtc
      181 gccggagtcg agccacaacg agcgcccggc cttggcccgc tcacgcttga tgatgtaggc
      241 catgcgggcg atcccgccgt agcgcccgcg ccccggcagc aggccgagct gctcctcggt
      301 gtacagcggg ttgtgctcgt acggcaggat ccgcgagtgg atgtccgagg tgtgcatgaa
      361 ggtcaggcgg atgtcctgat ccttgatgtt gggctcctcg gtctcgtaca ggcagccgcc
      421 ggtcgggacc gtgacggcgc agccgagggc gaacaaccat ggccgaagcc cggagcgcga
      481 cagggatggg attcgcgggg tcaacacggg cctcgaggag atcggatagc acccggtcgg
      541 gggtcggcgc aatcgcgcac gccggctccc cgacgcgccc ggcatgtgtt cgcgcatgcc
      601 cccgtgcgca gaccggggcg cagcgatcgg tgacgggccg cgcggggcgg ggcgcccgcg
      661 ccgggccggt cgttcatcgg cgcctcgccg aacgcagcag ggcgaactgg gtcgcgcccg
      721 ccaatgcgaa gccggtgaac gcgaggcagc cgagccactt ggtcacgaag gtgcgcaggt
      781 cggtgcccag cagctcggtc tgggtggtgg cgccgacggt gacgagcaac atgtcgacga
      841 cgaggaaggc gccgccggcg atcgcgatca agaggtaggg ccgggccagg ctgccgccga
      901 gcagcgggcg caccagccac acgaggtaca tgccgccggc gaacacgagc gcgtcgcaca
      961 gggtcgagac gacgatcgcg gcggcggcga accacgcccc gagggtcgca ggcacgccgc
     1021 gcccgacgag ctcgagggtg gggaaggcga gcatggcgag cgcgccggtc gccagcagcg
     1081 cgacgaaggt ggtcgcccgc acgcggtcct cggcgctgcg ctcctcgagc agctcggagc
     1141 tgccgagcac gcgaccgaag ccgatcacgg cggcggccat cgcgacgttg ccgacgatca
     1201 gcaggacgtg ggcggcggcg atccccgcga acttgacctg gaagtggctg agcaggcgca
     1261 cgaccgggag cagcgccatc gccgtggcga gcacggtcca cgggcgccgc ggcgggtcac
     1321 cggccgagaa gttgagggcg acggcgacgg tgacggcggc ggtgaggacc tcgacggcgc
     1381 gctgccatga ctcggcgctg tcggacccga gggtcatgtc gagcagcgcg cgaccgccga
     1441 cgagcgcggc gcccagcagc cacacggtgc ccggcgcgag acccggggcc cgcacggcgg
     1501 gacgcggctg cgaggccgcg gcgctcacga gacgacctcc gtgccgagat cggcgagcac
     1561 gcgcacggtg acgtcgttgc cgatcagcgt gcgcagcatc ggccgcaggg tggtgcaggc
     1621 gatcgccagg tcgtcggtgc cgagctcgct gggatccttg ccggcccgct tgcagatcat
     1681 cgccagggca cgcttcgcgg tgtgcggccc gagcagcggc gtcagcacct cgacgacgtg
     1741 ctcggcgaac tgcgccggca ctatgggctt gatgatcgac aaggcgtcac gcagggcccg
     1801 gggcccgtcg gccgatctta gccgatcccc gtccgtgaag gtcgcgcgct gcgaccatgg
     1861 gcagcacgcg tgtgacgggg gcagtgtgcc cctgagctaa cgcacgcgat tcgcgccgcg
     1921 catagtccaa ccgcgacgac ccgccgggct tgctgcgcgc acacgcgacc ttgatactcc
     1981 cgccggcatg gcgaccgaga tcaaggaagc gctgaccttc gacgacgtgc tgctggtgcc
     2041 ccgctacagc gaggtcttgc cgggtcaggt cgaggtcggc agtcgcctgt cgcgcggcat
     2101 caccttgaac atcccgctgc tgtcggcggc gatggacacg gtgacggagg cgcagacggc
     2161 gatctgcatg gcgcgtcacg gcgggctcgg cgtcgtccac aagaacatga cgccggagca
     2221 gcaggcgggg gaggtccggc gggtcaagaa gaccgagagc gggatggtcg tcgacccggt
     2281 gacgatcacg gcggagcaca cgctgcgcca ggcgctcgag atcatgcgcg cctgcggctt
     2341 cagcgggctg ccggtggtcc aggatcgcaa gccggtcggg atcctgacga gccgtgacct
     2401 gcgcttcgag accaacctcg accagccggt cggaggcgtg atgacgcgcg atccgatcac
     2461 ggtgccgcaa gggatcgcgc tcgaggatgc gcgcatcctg ttgcacaagc accggatcga
     2521 gaagctgctg gtcgtcgatc cccgcggcga gctgctcggg ctgatcacgg tcaaggacat
     2581 cctcaacgcg cagtcgcacc ccaacgcgat caaggacagc gccgggcgcc tgcgcgtcgg
     2641 cgcggcggtc ggggtcgggg ccgacctgat ggcccgcgtg caggcgctgc gcagcgagca
     2701 cgtcgacgtc atcgtcgtcg acaccgcgca cggtcactcg aagggggtgc tcgacgcggt
     2761 ccgcgggcta cgcggcgact tcccggacct ccagatcatc gccggtaata tcgcgaccgc
     2821 cgagggctac gcggcgctgt gcgacgccgg cgcggacggg gtcaagctcg gcatcgggcc
     2881 gggatcgatc tgcacgacgc gcgtggtcgc gggcgtcggc gtgccgcaga tcacggcgat
     2941 cctcgagtgt gccgcggtcg cgcgcaagcg cggggtgccg atcatctccg acggcgggat
     3001 caagtactcg ggcgatgtcg tcaaggcgct cgccgccggg gccgacagcg tgatggtcgg
     3061 ctcgctgttc gccggcaccg acgaggcgcc cggtgagctc gtgctctacc agggccgcag
     3121 ctacaaggtg taccgcggca tgggctcgct cggcgcgatg aaggccggca gcaaggaccg
     3181 ctacttccag ggcggcgtgt ccgacgagcg caagctggtc cccgagggca tcgagggccg
     3241 cgtgccgtac cgcgggccgc tgtcggactc gctgtatcag atggtcggcg gcctgcgctc
     3301 gggcatgggc tactgcggcg cgcgcacgat cgtcgatctc caggcgaccg ccgagctgcg
     3361 tcggatcacc agccagggcc tgcgtgagag ccacgtgcac gacgtgatca tcacgcaaga
     3421 ggcgccgaac taccgtcgag agtgaacacc atggccggcc atcagaccct catcgtcctc
     3481 gacttcggat cgcaggtcac ccagctcatc gcccgccgcg tgcgcgagct cggggtgttc
     3541 gcggagatcc tcccgtttca cacgccagtg gaacagctgc gagcgagaga cccgcaggcg
     3601 atcatcctca gcggcggccc gtcgtcgctg tacgacgagg gcgcgccgca gctcgatccg
     3661 gcggtgctgc agctcggcgt gccagtgctc gggatctgct acgggctgta cctgatcgtc
     3721 gccgggctcg gcggcacggt gatcccggcg acgcaccgcg agttcggccg cgccgacctc
     3781 gagatcgtcg cctccgaggg tccgctggcg cggttcggcg cgggcgagtc gctgcgcgtg
     3841 tggatgtcgc acggcgaccg cgtggagcgc atgccggccg ggtttcgtac ggtcggacgc
     3901 tcgccgcact gcgagcacgc ggcggtgtac gacccgcagc ggcgcctgtg gggcgtgcag
     3961 ttccacccgg aggtcacgca cagcgaccgc ggacaggacg tgctcggcgc gttcctcgag
     4021 acggcgggct ttcgcggcga ctggacgacg gcggcgttca tcgacgaggc ggtcgccaag
     4081 atccgcgccc gcgtcggcga gcacggcacg gtgctgtgcg ggctgtccgg cggggtcgac
     4141 tcgtcggtcg cggcgctgtt ggtggagcgg gcgatcgggc cgcgcctgca ctgcatcttc
     4201 gtcgacaatg ggctgttacg taaggacgag cgcgccgagg tcgagaagct gttcgccggg
     4261 cgcctcgcgc agccgctggt gacggtcgac gcgagcgacc gcttctacgg cgagctgacc
     4321 agcgtgatcg accccgagca gaagcgcaag cgcatcggcg cggccttcat cgacgtgttc
     4381 gaggcggagg cccagcgcct cggcggcgcg gactttttgg tgcagggcac gctgtacccg
     4441 gacgtgatcg agtcggtgaa cctgcgtggc gcgtcggtca cgatcaagac gcatcacaac
     4501 gtcggcggcc tgcccgagcg catgcgcatg ggcgtggtcg aaccactgcg cgagctgttc
     4561 aaggacgagg tgcgcaagct cggcgcggag ctcgggctgc cggacgacat ggtctggcgc
     4621 cacccgttcc ccggccccgg cctggcggtg cgggtgctcg gcgaggtgac gaagcagcgc
     4681 tgcgacctgc tgcgcgacgc cgacgcgatc gtgatcgagg aggtccgccg cgccggcctg
     4741 tatcgtgagc tctggcaggt gttcgcggtg ctgctgccgg tccagagcgt cggcgtgatg
     4801 ggcgacggcc gcacctacga gaacgcgctg gcgatccgcg cggtcaccag caccgacgcg
     4861 atgacggccg actgggcccg gctgccctac gacctgctcg acgtcatgag tcggcgcatc
     4921 atcaacgagg tccgcgggat caaccgcgtc tgctacgaca tcagcagcaa accgccggcc
     4981 acgatcgagt gggagtaaca gcatgcgcgt gccgcacgac gacggcgccg gaccgaagaa
     5041 gcccggcgcg aagccgaggg ccaaggccgc tccggagatc gtgatgcccg gtgaaaagga
     5101 gggggcgaag ccggccccgc tcgagcgcgc gctggtcacc ggcgtgcgcg cgcagggtga
     5161 cgccgaggcg ccgaacctgc gaccgcagcg cttcgccgag tacatcggcc aggcggcgct
     5221 caagcgcaag ctcgaggtgt tcgtgcgcgc ggccaaggca aggggtgagc cgctcgacca
     5281 cacgctgctg cacggcccgc ccggcctcgg caagacgacg atggcgcaca tcctcgcgca
     5341 cgagctcggg gtcaagctgc acctgacctc gggcccggcg atcgaacaca aagggatcct
     5401 cgccggccac ctgacggcgc tcgagccgag ggacgtcctg ttcatcgacg agatccaccg
     5461 cctgtcgccg acggtcgagg agagcctgta ctcggcgatg gaggacggtc gcatcgacct
     5521 gccggtcggc gaggggaccc gcgcgcgcac gatcggcttc gagctcgcgc cgtttac
//
LOCUS       DASWNJ010001825         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_67669, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001825 DASWNJ010000000
VERSION     DASWNJ010001825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67669"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /locus_tag="VGB85_30550"
     CDS             complement(<1..1330)
                     /locus_tag="VGB85_30550"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0138480.1"
                     /translation="MPSPSRAPGYLPTRRAVKALVKVGYGCNEHCTFCHTLDVREIQG
                     ASAEVEAKILRASVLFREQLPPGEVGMVVLSGGEPTIRPELLRWADLVASLDMDLGLV
                     TNGLMLAYPEQVEALLRRRLRYVYLSLHGGSARVHNLMVRTHAFDAAHAALRNLAGRG
                     LDLHVNCVVTKHNLAHLRGLVDAVLPFPDATLKFSMVEPKGGGDRLFNHIMPRVAEVA
                     EKVLAAIAYGESKASPQGPRFEHGGLPLCLLPGRETQYGDLRTHRYRTMIEVGEPDYF
                     PVDDLNKLHPPACDGCSLRGACPGLYRGYHEVHGDAELRPRPGPRANAFNYTLRGLVA
                     VPEDMSEGQCPLRDGSLGVTPWDRGRDLFVRHRGKLAHYRADTRDFSDAEIAEIKHTR
                     AQVYVDASRKDAPDDFAKDLVPLARASLCAGCPHAARCAGIHEPVLEDIFT"
     gene            1467..1871
                     /locus_tag="VGB85_30555"
     CDS             1467..1871
                     /locus_tag="VGB85_30555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138481.1"
                     /translation="MQVPAGERFELLGESLAAGTSLGHGSIDSVLPLTAGAVPVVMRT
                     PDGERFQVDILRRDPRGPAGVADTARLSLFVVNRGDGATRTHEAQAHAARALAAWLGQ
                     RETDATVAGLGELLSHPERQRAHPAGVFSLWR"
     gene            1862..>2432
                     /locus_tag="VGB85_30560"
     CDS             1862..>2432
                     /locus_tag="VGB85_30560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138482.1"
                     /translation="MALSPGFAGHAGLALTEAPAASIVRGDRTVMWFARAGEDWPDQA
                     VTHLHLWLHAAQVPAHEFHSGVVGSFAAIAAQIDRARTLGITVAVSSLLTRSNARVLA
                     ELPPWLQARGVRAWRVAVPWVVGTVRPGALAGVGALDGLVPRLGAALPYALQALAGAG
                     RVGVSAGIGGAPLCLLGPFVATAISEPARA"
BASE COUNT          298 a          766 c          994 g          374 t
ORIGIN      
        1 tggtaaagat gtcctcgagg acaggctcgt ggatgccggc gcagcgggcc gcgtgcgggc
       61 agccggcgca cagcgaggcg cgggcgagcg gcacgaggtc tttggcaaaa tcgtcggggg
      121 cgtccttgcg gctggcgtcg acgtagacct gcgcgcgggt gtgcttgatc tccgcgatct
      181 cggcgtcgct gaagtccctg gtgtcggcgc ggtagtgggc gagcttgccg cggtggcgga
      241 cgaacaggtc gcggccgcgg tcccacgggg tcacgccgag ggagccgtcg cgcaggggac
      301 attgcccttc ggacatgtcc tccgggacag cgacgaggcc gcgcagggtg tagttgaagg
      361 cgttggcgcg cgggccgggg cgcggccgca gctcggcgtc gccgtggacc tcgtggtagc
      421 cgcggtacag gccggggcag gcgccgcgca gggagcagcc gtcacaggcg ggcgggtgca
      481 gcttgttgag gtcgtcgacc gggaagtagt cgggctcgcc gacctcgatc atcgtgcgat
      541 agcggtgggt gcgaaggtcg ccgtactggg tctcgcggcc gggcagcagg cacagcggca
      601 ggccgccgtg ctcgaagcgc gggccttgcg ggctcgcctt gctctccccg taagcgatcg
      661 cggcgagcac tttttcggcg acctcggcga cgcgcggcat gatgtggttg aacaggcgat
      721 caccgccgcc cttgggctcg accatcgaga acttgagcgt cgcgtcgggg aagggcagca
      781 cggcgtcgac caggccgcgc aggtgggcga ggttgtgttt ggtgacgacg cagttgacgt
      841 gcaggtcgag gccgcggccg gcgaggttgc gcagggccgc gtgcgcggcg tcgaaggcgt
      901 gcgtgcggac catcaagttg tgcacgcggg cgctgccgcc gtgcaacgag aggtagacgt
      961 agcgcaggcg cctgcgcagc agcgcctcga cctgctcggg gtaggcgagc atcaggccgt
     1021 tggtgacgag gccgaggtcc atgtcgaggc tggcgacgag gtcagcccag cgcagcagct
     1081 cggggcgaat ggtcggctca ccgcccgaga gcacgaccat gccgacctcg ccgggtggca
     1141 gctgctcacg aaacaggacc gaggcgcgca ggatcttggc ctcgacctcg gcgctggcgc
     1201 cctggatctc gcggacgtcg agggtatggc aaaaggtgca gtgctcgttg cagccgtagc
     1261 cgaccttgac aagcgccttg acggcccgcc gcgtcggcag gtagcctggt gctcggctgg
     1321 gagaaggcat gccacaggtt tcgcgcaggc tgctatgtcg acgcaaggtc cttatcgccg
     1381 cgggtgtggc ggtcgtcggc gcgtccgtcg gattttcgcc ggcgagggcc gctgcccgtg
     1441 aacgatcgcg tggcggtcgt gtacgcttgc aggtgcccgc gggggagcgc ttcgagctgc
     1501 tcggcgagtc gctggcggcc ggcacctcgc tgggccatgg gagcattgat tcggtgctgc
     1561 cgttgacggc gggggcggtc cccgtggtga tgcgcacgcc ggacggcgag cgcttccagg
     1621 tcgatattct tcgcagagat ccgcgtggcc cggccggcgt cgccgacacc gcgcggctga
     1681 gcctgttcgt cgtcaaccgt ggtgacggtg ccacgcgcac gcacgaggcc caggcccacg
     1741 cggcgcgggc gctggctgcg tggctcggcc agcgtgagac ggacgcgacg gtggcggggc
     1801 tcggcgagct gctgagtcac cccgagcgcc agcgggccca ccccgcgggt gtgttctcgc
     1861 tgtggcgctg agcccgggct tcgcgggcca cgcggggctg gcgttgaccg aggcgcccgc
     1921 ggcctcgatc gtccgcggtg atcgaaccgt gatgtggttc gcgcgcgccg gggaggactg
     1981 gccggaccag gccgtcacgc acctgcatct gtggctacat gccgcgcagg tcccggcgca
     2041 cgagttccat agcggcgtcg tcgggagctt cgccgcgatc gcggcgcaga tcgatcgggc
     2101 gcgcacgctg gggatcacgg tcgcggtgtc gtcgctgctg acgcgctcga acgcgcgggt
     2161 gctcgcggag ctgcctccgt ggttgcaggc gcgtggcgtg cgggcctggc gggtcgcggt
     2221 gccgtgggtg gtcgggacgg tgcggccggg ggccctggcg ggggtcgggg cgctcgatgg
     2281 cctcgtgccg cgcctcggcg ccgcgctgcc gtatgcattg caggcgctcg cgggcgcagg
     2341 gcgggtcggt gtcagcgcgg ggatcggcgg ggcgccgctg tgcctgctcg ggccgttcgt
     2401 tgcgaccgcg atcagcgagc cggcgcgcgc gt
//
LOCUS       DASWNJ010001826         3229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_103734, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001826 DASWNJ010000000
VERSION     DASWNJ010001826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103734"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /gene="obgE"
                     /locus_tag="VGB85_30565"
     CDS             complement(<1..1011)
                     /gene="obgE"
                     /locus_tag="VGB85_30565"
                     /inference="COORDINATES: protein motif:HMM:NF008956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY0138483.1"
                     /translation="MRFIDQVRIVVRAGKGGDGLVGWRREKFVPDGGPAGGDGGNGGD
                     VIFVADDHLTSLLDLKFRQHFAAESGKNGGPNKMAGRAGGDLKVRVPVGTMVFLEGLA
                     GEPGERPPWMADEDGEAEDDGAMQNYGMVAWEDDGEEDPNAPDPQPALRQKYLTRKSR
                     HRQPEAELPPEPGDLLADLTTPGQELVIAKGGRGGRGNVHFRSSTNRAPDHAEPGIPG
                     EAYWLRLELKLMADVGIVGFPNVGKSTFIRAISRARPKVGDYPFTTLVPQLGVASLPG
                     ERSLVVADVPGLIRGASEGKGLGLEFLRHVERCAVLVHVIDTMTYESGRDPLTDLDVI
                     EAE"
     gene            complement(1032..1325)
                     /gene="rpmA"
                     /locus_tag="VGB85_30570"
     CDS             complement(1032..1325)
                     /gene="rpmA"
                     /locus_tag="VGB85_30570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY0138484.1"
                     /translation="MAHKKGQGSIKNGRDSKSQRRGVKKFGGEVVRAGNILVRQVGSS
                     FHAGNNVGTGKDYTLFALAAGTVRFYKRGHERKTFVAIDPLAAEGADLASAAE"
     gene            complement(1450..1770)
                     /gene="rplU"
                     /locus_tag="VGB85_30575"
     CDS             complement(1450..1770)
                     /gene="rplU"
                     /locus_tag="VGB85_30575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY0138485.1"
                     /translation="MSELQQAIIRTGGKQYRVSPGSVLKVEKLPVAQGAAVEFEVLLA
                     GTGEHVQIGTPLVAGARVTGEVVSHGRGVKIIVYKFRKRANYRKKQGHRQAYTEVRIT
                     AIHG"
     gene            complement(2004..>3229)
                     /locus_tag="VGB85_30580"
     CDS             complement(2004..>3229)
                     /locus_tag="VGB85_30580"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY0138486.1"
                     /translation="EPSAAWEDIEGNQYPRKHTLYLNFNGGVLYGGEDNSAEDKSALA
                     RAGQPYPGWNKAESTALAVIQRVKEDMATLGVRVAYDKRPNKTVPYTMAMVSGDWTDV
                     NIDSPAGGVAPGTDCEARGQRHVVYVFDNQASTVGQEAAHSWGLDHTLGGDRIMSYQF
                     GTNKHFGDNCQALCEEACQGKGSIGCRLIHEKYCGVDSEQQNDLAELSFIFGGNEPDT
                     EPPTVKITNPPDGTVLAVGESTQLVGEVHDNYGGVGWKLTIVVDGEVKLDQVDYDRAL
                     SWSLQKLPAGVYEITLEAEDHADHIVRDTITITVGNPAPPDTDTDGTGTGSDSNSGSD
                     SGDTPTTSDTDDSAGTGGGETDAGSDASGEGSDTSSEDDGGGCRISSGSQGPLGLLVP
                     VLGLGLAWRGRRRRA"
BASE COUNT          443 a         1092 c         1151 g          543 t
ORIGIN      
        1 ctcggcctcg atcacgtcga ggtcggtcag cgggtcgcgc cccgactcgt acgtcatggt
       61 gtcgatgacg tggaccagca cggcgcaccg ctcgacgtga cgcaggaact cgaggccgag
      121 gcccttgccc tcgctggcgc cgcggatcag gcccggcacg tcggcgacga cgagcgaccg
      181 ctcgcccggg agcgacgcga cgccgagctg cggcacgagc gtggtgaacg ggtagtcgcc
      241 gaccttgggc cgcgcgcggg agatcgcgcg gatgaaggtc gacttgccga cgttggggaa
      301 gccgacgatg ccgacatcgg ccatcagctt gagctcgagg cgcagccagt acgcctcgcc
      361 ggggatgccg ggctcggcgt ggtcgggcgc gcggttggtc gagctgcgaa agtgcacgtt
      421 gccgcggccg ccgcgaccac ccttggcgat caccagctcc tggcccggcg tcgtcaggtc
      481 ggcgagcagg tcacccggct cgggcggcag ctcggcctcc ggctggcggt ggcggctctt
      541 gcgggtcagg tacttctgac gcaacgcggg ctgcgggtcg ggggcgttgg ggtcctcctc
      601 gccgtcgtcc tcccacgcga ccatgccgta gttctgcatg gcgccgtcgt cctcggcctc
      661 gccgtcctcg tcggccatcc acggcggccg ctcgccgggt tcaccggcga ggccctcgag
      721 gaagaccatc gtgccgaccg gcacacgaac cttgaggtca ccgccggcgc ggccggccat
      781 cttgttgggg ccgccgtttt tgcccgactc cgccgcgaag tgctggcgga acttcaggtc
      841 gagcaaggag gttaaatggt catcggcgac gaagatgaca tctccgccgt tgccgccgtc
      901 accgccggcg gggccgccgt cggggacgaa tttctcgcgc cgccagccga ccaggccgtc
      961 accgccctta ccagcgcgga cgacgatacg aacctggtcg atgaaacgca tggacgatcc
     1021 gggcctacgg cctactcggc cgcggacgcc aggtcggcgc cttcggccgc cagcgggtcg
     1081 atggcgacga aggtcttgcg ctcatggcca cgcttgtaga agcggaccgt gcccgccgcg
     1141 agggcgaaca gcgtgtagtc cttgccggtg ccgacgttgt tgccggcgtg gaagctgctg
     1201 ccgacctggc gcacgaggat gttgcccgcg cgcacgacct cgccgccgaa cttcttgacg
     1261 ccacgtcgct gggacttgga gtcgcggccg ttcttgattg aaccttgacc ttttttgtgt
     1321 gccatcgtgc tctctcctgc cgcggcctgc cccgaaggtc aggcacatcc ggcgaaaaac
     1381 tcaaacgttc gctgtgaacc ggaggccccc gtggaggcca ggaagatcaa ccgaaagatc
     1441 aaccgtagat caaccgtgga tcgcggtgat ccgaacctcg gtgtaggcct ggcggtggcc
     1501 ctgcttcttg cggtagttgg cccgcttacg aaacttgtag acgatgatct tgacgccacg
     1561 accgtggctc acgacctcgc cggtgacgcg ggcgccagcg acgagggggg tgccgatctg
     1621 gacgtgctcg ccggtgccgg cgagcaggac ctcgaactcg accgcggcgc cctgggcgac
     1681 gggcagcttc tcgaccttga ggacggagcc ggggctgaca cggtactgct tgccgccggt
     1741 gcggatgatg gcttgctgaa gctcgctcac gggaaactca attctttcag gcgatgcgcc
     1801 cgcccggggc ggcaaccgtc gagccggctg cgactgctcg gtgatgaggt gacagagggg
     1861 ccctcgccga gcgcgagggg acatggccta taacgggatc caccccgggg ggtcaagccg
     1921 cgcgcatgcg gccgaggtca cattttttcg cggatgatac ggataatgcg ccgacgagcc
     1981 cccgcggagc ccgccggagg ccctcaggcg cgccgacggc ggccccgcca ggccaggccc
     2041 agcccgagga cggggaccag caggccgagc ggtccctgcg aaccggagga gatccggcag
     2101 ccgccgccgt cgtcctcgct ggaggtatcg ctgccctcgc cgctcgcgtc ggagccggcg
     2161 tcggtctcgc cgccgccggt cccggcgctg tcgtcggtgt ccgaggtggt cggcgtgtcg
     2221 ccggagtccg agcccgagtt ggagtccgag ccggtgccgg tgccgtccgt gtccgtgtcc
     2281 ggcggcgcgg ggttgccgac ggtgatcgtg atcgtgtcgc ggacgatgtg gtcggcgtgg
     2341 tcctcggcct cgagcgtgat ctcgtagacg ccggcgggca gcttctgcag gctccagctg
     2401 agggcgcggt cgtagtcgac ctggtcgagc ttgacctcgc cgtcgaccac gatcgtcagc
     2461 ttccagccga cgccgccgta gttgtcgtgc acctcgccga cgagctgcgt cgactcgccg
     2521 accgcgagca ccgtgccgtc cggcgggttg gtgatcttca ccgtgggcgg ctcggtgtcg
     2581 ggctcgttgc cgccgaagat gaagctgagc tcggcgaggt cgttctgctg ctcggagtcg
     2641 acgccgcagt acttctcgtg gatcaggcga cagccgatcg atcccttgcc ctggcaggcc
     2701 tcctcacaca gcgcctggca gttgtcgccg aagtgcttgt tggtgccgaa ctggtagctc
     2761 atgatgcggt cgccgccgag cgtgtggtcg aggccccacg agtgcgcggc ctcctgaccg
     2821 accgtcgagg cctgattgtc gaacacgtag acgacgtggc gctggccgcg ggcctcgcag
     2881 tcggtgcccg gggcgacgcc gccggccggg ctgtcgatgt tcacgtcggt ccagtcgccg
     2941 ctgaccatcg ccatcgtgta cggcacggtc ttgttgggcc gcttgtcgta ggcgacgcgc
     3001 acgccgagcg tcgccatgtc ctccttgacg cgctggatca ccgcgagggc ggtcgactcg
     3061 gccttgttcc accccgggta aggctggccg gcccgcgcca gggccgactt gtcctcggcc
     3121 gagttgtcct cgccgccgta cagcacgccg ccgttgaagt tgaggtacag cgtgtgcttg
     3181 cgtggatact ggttgccctc gatgtcctcc cacgcggccg agggctcgg
//
LOCUS       DASWNJ010001827         2368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_526231, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001827 DASWNJ010000000
VERSION     DASWNJ010001827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526231"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..1137)
                     /locus_tag="VGB85_30585"
     CDS             complement(10..1137)
                     /locus_tag="VGB85_30585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138487.1"
                     /translation="MQSEFDTTTKLLFVLTGFMAIMVGLIAYVAVFKQPDPLLDGPAR
                     AEAPDATEKPEGERCDATSLEGATCPDGKYCRFDTCVPITQSNLCAEGESCRDCECEQ
                     GLLCHQFRCTQESKVDKTPLVCAENPALSNAVRSLATKCASRKTSVDKIASTGSCTTA
                     DWEALALEDEKFDLLLSAFPNRMAIHFPPGQPHPRKKGWPTPAVRTHLLAQFRQFQTA
                     LRSSKQVFVIGRASPDGDPKDNHILAVKRMDLASNLIEAILYEGVPETERDAHRVPIR
                     SFTLPTTNPINPERYRKSYLNNPDGSRPAISPIIAWDEVNQRSLQTALDGGLDLADRN
                     SSEWQYLYGAINRVVMIIPIPCLGDEYEQRKTILDRPVSKG"
     gene            complement(1142..>2368)
                     /locus_tag="VGB85_30590"
     CDS             complement(1142..>2368)
                     /locus_tag="VGB85_30590"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEY0138488.1"
                     /translation="LGVSGYLTLLKFRMLYTPCLSARGGCNVGGLTCEDALGSTWSML
                     MGLPISVWGAAFYAATAVLAIGLLRRPDFLAGAAPGLLLRLAIFDVLVSVGYAIYAFG
                     VLGSPCPFCLSLYVISGLLLGAVVLLRRGHPTRAEGTALTRLRQASVLDAAFMAAVVF
                     VVAAGVQSVGYQLSRRFVDAQSGCPEKIEPLPPATIKIGASDPKAIVALFIDMSCSHC
                     KAEFKVVVNALNNAQFPDPTQLWIYHTPRQACDPEAFAGGYSKSDDNVRYDNPCLAAR
                     AAECMEKLQPGAGIELIGGMYVLHDTREANTPLFTAERIGNKAVELEMQIDPDDPDNK
                     LFRCINDDRAVIDLISAHQRYAEGPLFKVPTAAVYSAVNGQPDMTRKPLYGDANTPID
                     ALAAYIHTQATPPAGT"
BASE COUNT          342 a          754 c          885 g          387 t
ORIGIN      
        1 tggcggctcc taacccttgc tgaccggacg gtcgaggatc gtcttgcgct gctcgtactc
       61 gtcgcccagg caggggatcg ggatgatcat gaccacgcgg ttgatcgccc cgtagaggta
      121 ctgccactcg ctcgagttgc ggtccgcgag gtcgaggccg ccgtcgagcg cggtctgtag
      181 gctacgctgg ttgacctcgt cccacgcgat gatcgggctg atcgccgggc gcgagccgtc
      241 cgggttgttg aggtagctct tgcggtagcg ctcggggttg atcgggttgg tggtcggcag
      301 cgtgaacgac cggatcggca cgcggtgagc gtcgcgctcc gtctcgggca caccctcgta
      361 gaggatcgcc tcgatgaggt tgctcgcgag gtccatgcgc ttgacggcga ggatgtgatt
      421 gtccttgggg tcgccgtcgg ggctcgcgcg accgatcacg aagacctgct tcgagctgcg
      481 cagcgcggtc tggaactggc gaaactgggc gagcaggtgg gtgcggaccg ccggcgtcgg
      541 ccagcccttc ttgcgcgggt gcggctggcc cggcgggaag tggatcgcca tgcggttggg
      601 gaaggccgac agcagcaggt cgaacttctc gtcctcgagc gcgagcgcct cccagtcggc
      661 cgtcgtgcac gagccggtcg aggcgatctt gtcgacgctg gtcttgcggc tcgcgcactt
      721 ggtcgccagc gagcgcacgg cgttcgacag cgccgggttc tcggcgcaca ccagcggcgt
      781 cttgtcgacc ttggactcct gcgtgcagcg aaactggtgg cacagcaggc cctgctcgca
      841 ctcgcagtcg cggcagctct cgccctcggc gcacaggttg ctctgcgtga tcgggacgca
      901 ggtgtcgaag cggcagtact tgccgtccgg gcaggtcgcg ccctcgaggc tggtcgcgtc
      961 gcagcgctcg ccctcgggct tctcggtcgc gtccggggcc tcggcgcgcg ccgggccgtc
     1021 gagcagcggg tcgggctgct tgaacaccgc gacgtaggcg atcaggccga ccatgatcgc
     1081 catgaagccg gtgaggacga acagcagctt ggtcgtggtg tcgaactcgg attgcatgcg
     1141 atcaggtgcc ggcgggagga gtggcctggg tgtggatgta ggcggcgagc gcgtcgatcg
     1201 gcgtgttggc gtcgccgtac agcggcttgc gcgtcatgtc gggctggccg ttcacggcgc
     1261 tgtacaccgc cgcggtcggg accttgaaca gcgggccctc ggcgtagcgc tggtgcgcgg
     1321 agatcaggtc gatgacggcg cggtcgtcgt tgatgcagcg aaacagcttg ttgtccgggt
     1381 cgtcggggtc gatctgcatc tcgagctcga cggccttgtt gccgatccgc tccgcggtga
     1441 acagcggggt gttcgcctcg cgcgtgtcgt gcagcacgta catgccgccg atcagctcga
     1501 tgccggcgcc gggctggagc ttctccatgc actcggcggc ccgcgccgcc aggcacgggt
     1561 tgtcgtagcg gacgttgtcg tcggacttgc tgtagccgcc cgcgaacgcc tcgggatcgc
     1621 aggcctggcg cggcgtgtgg tagatccaca gctgcgtcgg gtcggggaac tgggcgttgt
     1681 tgagggcgtt gacgacgacc ttgaactccg ccttgcagtg gctgcacgac atgtcgataa
     1741 acagcgcgac gatcgccttg gggtcgctcg cgccgatctt gatcgtcgct ggcggcagcg
     1801 gctcgatctt ctccgggcag ccggactggg cgtcgacgaa gcggcgtgac agctggtaac
     1861 cgaccgactg cacgccggcc gcgacgacga acacgaccgc ggccatgaac gccgcgtcga
     1921 gcacggaggc ctggcgcagc cgcgtcagcg cggtcccctc ggcgcgcgtc ggatgcccgc
     1981 ggcgcagcag cacgacggcg ccgagcagca ggccgctgat gacgtacagc gacaggcaga
     2041 acgggcacgg cgagccgagc acgccgaacg catagatcgc gtagccgacg ctgacgagca
     2101 cgtcgaagat cgccaggcgc aacagcagcc cgggcgccgc gccggcgagg aagtcgggcc
     2161 ggcgcagcag gccgatcgcc agcacggcgg tggcggcgta gaaggccgcc ccccacacgc
     2221 tgatcggcag gcccatcagc atcgaccagg tcgagccgag cgcgtcctcg caggtcaggc
     2281 cgccgacgtt gcagccgccg cgcgcgctga ggcacggcgt gtagagcatg cgaaacttga
     2341 ggagggtcag gtaaccgctg acgccgag
//
LOCUS       DASWNJ010001828         4078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_454471, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001828 DASWNJ010000000
VERSION     DASWNJ010001828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454471"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /gene="rplA"
                     /locus_tag="VGB85_30595"
     CDS             <1..571
                     /gene="rplA"
                     /locus_tag="VGB85_30595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEY0138489.1"
                     /translation="RLGVNPKHADQMIRSSCALPNGTGKTLRVVVFAKGDRAAEARDA
                     GADFVGAEDLVERVLKENWLDFDSAIATPDMMGLVGRLGRILGPRNLMPNPKVGTVTM
                     DVGRAIREIKAGRIEFRTEKTGIVHAPIGKLSFGADKLRENLTSLLETLQRLKPSTAK
                     GKYFKSVALSTTMGPSVRLDTNDVAALVE"
     gene            800..1318
                     /gene="rplJ"
                     /locus_tag="VGB85_30600"
     CDS             800..1318
                     /gene="rplJ"
                     /locus_tag="VGB85_30600"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEY0138490.1"
                     /translation="MLRLTKHEASSRLREALSDVPAFILLDYTGITVSQDTDLRNKFR
                     EGQCKYQVFKNSVVHYAIQGTRHEPVSRLLTGMTALAYSPTDPGAAARIARDFARDAK
                     NFKIKGGVIEGTVLDAAGVNSLADMPGPRELKAQFLALLNTPATSFVRVLNAGPQSLL
                     YLLNARKDQLAA"
     gene            1402..1788
                     /gene="rplL"
                     /locus_tag="VGB85_30605"
     CDS             1402..1788
                     /gene="rplL"
                     /locus_tag="VGB85_30605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEY0138491.1"
                     /translation="MSTVTRDQVIDFLSNLPVIEISNLVKELEAKWGVSAAAPVAAAG
                     PAAAAGPAAPAAEAQTEFTVVLKGFGDKKINVIKAVRTINPALGLKEAKDLVEALGNI
                     KEGISKAEADEMAKTLREAGAEIDIK"
     gene            2155..>4078
                     /locus_tag="VGB85_30610"
     CDS             2155..>4078
                     /locus_tag="VGB85_30610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEY0138492.1"
                     /translation="MPPMIQNNFRVRKNFGKLKRIIEVPNLIDIQKRSYDKFLQRDTP
                     LESREDIGLQAVFKSVFPIKDFGETSSLEFVSYSLDRPKYDEDECRARGMTFAAPIKV
                     VVRLVVWDVDDETGTQSIRDVKEQEVYFGEIPLMTAHGTFIINGTERVVVSQLHRSPG
                     VFFDHDKGKTHSSGKKLFSARVIPYRGSWLDFEFDHKDHIYVRIDRRRKLYATVLLRA
                     LGLSTQQLLDFYYEKENIHLEDNGDITRAIDFELLSGQRASHDIRDPETGETVVKKGK
                     KFSKAHIKRMRASGIKRLPMESAGLVGKVSAEDIIDENTGEVLLNCNEDINEEHLIRL
                     RSAGIKSFKILFIDDFNVGPFLRNTLLQDKLHTPEEALLEIYRRLRPGDPPTAETART
                     LFESLFFKPERYDLSTVGRLKLNHKFSLDEALMSTVLTKRDIMETVKYLVELRNGRGQ
                     IDDIDHLGNRRVRAVGELMENQYRIGLVRMERAIKERMSMSQEMDALMPADLINAKPV
                     SAVVKEYFASSQLSQFMDQTNPLSEVTHKRRLSALGPGGLTRERAGFEVRDVHSTHYG
                     RICPIETPEGPNIGLIASLSTYARINEFGFIETPYRAVDGGVASNEVQYYSALQEAGH
                     YIAQANAPIDESGKLLA"
BASE COUNT          773 a         1502 c         1178 g          625 t
ORIGIN      
        1 ccgcctgggc gtcaacccga agcacgccga ccagatgatc cgctcgagct gcgccctgcc
       61 caacggcacc ggcaagaccc tgcgcgtcgt cgtgttcgcc aagggtgacc gcgccgccga
      121 ggcgcgtgac gccggcgccg acttcgtggg cgccgaggac ctggtcgagc gcgttctcaa
      181 ggagaactgg ctcgacttcg acagcgccat cgccacgccc gacatgatgg gcctggtcgg
      241 tcgcctcggc cgtatcctcg gaccgagaaa cctgatgccg aaccccaagg tcggcacggt
      301 gaccatggac gtcggtcgcg ccatccgcga gatcaaggcc ggtcgcatcg agtttcgcac
      361 cgagaagacc ggcatcgtcc acgcgccgat cggcaagctc tcgttcggcg ccgacaagct
      421 gcgcgagaac ctgacctcgc tgctggagac cctccagcgc ctcaagccct cgaccgccaa
      481 gggcaagtac ttcaagagcg tcgcgctctc gaccacgatg ggcccgtccg tccgcctgga
      541 caccaacgac gtcgccgcgc tcgtcgagta gctcgtccat ttttcgtttt cttcaggagg
      601 ctgtcgccac cgaggtgatc gtcagagagc gcccgagtcc cgggcaacac actcgcagac
      661 agcaggtggt cggccagccc gaccttgaac gccagagagc ggcccgccga gagtggagcg
      721 aacgttgaga ggaagggtgc gccgccgcac ccgatctccg cggtcctccc tccggaggac
      781 caaggagaac caaaagacca tgctgaggct taccaaacac gaggcatcga gccggttgcg
      841 cgaggcgctg agcgacgtgc cagcgttcat cctgctcgac tacacgggca tcaccgtttc
      901 ccaggacacg gacctgcgca acaagttccg cgagggacag tgcaagtacc aggtcttcaa
      961 gaactcggtc gtgcactacg ccatccaggg cacccgccac gagcccgtct cgcggctgct
     1021 gaccgggatg accgcgctgg cctactcgcc gaccgatccg ggcgccgccg cccgcatcgc
     1081 ccgcgacttc gcccgtgacg ccaagaactt caagatcaag ggcggcgtca tcgagggcac
     1141 cgtcctcgac gcggcaggcg tcaacagcct cgccgacatg cccgggccgc gcgagctcaa
     1201 ggcccagttc ctggcgctcc tcaacacccc ggcgacgagc ttcgtccgcg tgctcaacgc
     1261 cggcccgcag agcctcctct acctcctcaa cgcccgcaaa gaccagctcg ctgcctgagc
     1321 tggcactggc cccgccgact cgcccgtcga ccggtccagc cctgacttcc acccccaaca
     1381 gaaccttcga aggaatccgc catgtccacc gttacccgtg atcaagtcat cgacttcctg
     1441 agcaacctcc cggtcatcga gatctccaat ctcgtcaagg agctcgaggc caagtggggc
     1501 gtctccgccg ccgctccggt cgccgctgcc ggccccgccg ccgctgccgg ccccgctgcc
     1561 cccgccgccg aggctcagac cgagttcacc gtcgtcctca agggcttcgg cgacaagaag
     1621 atcaacgtca tcaaggccgt ccgcaccatc aaccccgccc tcggcctcaa ggaggccaag
     1681 gacctggtcg aggccctcgg caacatcaag gaaggcatct ccaaggccga ggccgacgag
     1741 atggcgaaga ctctccgtga ggcgggcgct gagatcgaca tcaagtgagc acgtggcgcc
     1801 tcggcgtccg ttcaagcttc cgcgtgtatg gcgccaggag gtgactcctg gtgccgtaca
     1861 cccgtcgcat tttcgctgga gcaatttcgc gcctgtgacc atcccggcca ccctttgaaa
     1921 tctcaaatgg gcctgatctc acatcgctcc acgtcgaatc gtccctgatc cgttcgctgc
     1981 ttaatcactt cgcggcccct gggcccgagg ccattcctta gacaatctgc acgccccccg
     2041 acggtccgtc cggacccgac ccgacctgcc tctcgcctga ccgcctggcc cccgctgcac
     2101 cgcgccattc gtgccaccgc agcaaccccg cccaggcctc atcaaggaac gccgatgcca
     2161 ccgatgattc agaacaactt cagggtccgc aagaacttcg gaaagctcaa gcggatcatc
     2221 gaggtaccca atctcatcga catccagaag cggtcgtacg acaagtttct gcagcgcgac
     2281 actcccctgg agtcgcgcga ggacatcggc cttcaggccg tcttcaagag cgtcttcccg
     2341 atcaaggact tcggcgagac ctcgtcgctc gagttcgtca gctacagcct cgaccgcccc
     2401 aagtacgacg aggacgagtg tcgcgcccgc ggcatgacct tcgcggcgcc gatcaaggtc
     2461 gtcgtccgcc tcgtcgtgtg ggacgtcgac gacgagaccg gcacccagtc gatccgcgac
     2521 gtcaaggagc aggaggtcta cttcggtgag atcccgctga tgacggcgca cggtaccttc
     2581 atcatcaacg gcaccgagcg cgtcgtcgtc tcgcagctgc accgctcgcc cggcgtgttc
     2641 ttcgaccacg acaagggcaa gacccacagc tccggcaaga agctgttctc cgcccgcgtg
     2701 atcccgtacc gcggctcgtg gctcgacttc gagttcgacc acaaggacca catctacgtc
     2761 cgcatcgatc gccggcgcaa gctgtacgcg actgttctcc tgcgcgcgct cggactctct
     2821 acccagcagc tgctcgactt ctactacgag aaggagaaca tccacctcga ggacaacggc
     2881 gacatcaccc gcgccatcga cttcgagctg ctcagtggcc agcgcgcctc gcacgacatc
     2941 cgcgacccgg agaccggcga gaccgtcgtc aagaagggca agaagttcag caaggcgcac
     3001 atcaagcgca tgcgcgcctc gggcatcaag cgcctgccga tggagtccgc cggcctggtc
     3061 ggcaaggtct cggccgagga catcatcgac gagaacaccg gcgaggtgct cctcaactgc
     3121 aacgaggaca tcaacgagga gcacctgatc cgcctgcgct cggccggcat caagagcttc
     3181 aagatcctct tcatcgacga cttcaacgtc ggcccgttcc tgcgcaacac cctgctgcag
     3241 gacaagctgc acacccccga agaggccctg ctcgagatct accgccgcct gcgtcccggc
     3301 gatccgccga ccgccgagac cgctcgcacg ctgttcgagt cgctgttctt caagcccgag
     3361 cgctacgacc tctccacggt cggccgcctc aagctcaacc acaagttcag cctcgacgag
     3421 gccctgatgt cgaccgtgct gacgaagcgc gacatcatgg agaccgtcaa gtacctggtc
     3481 gagctgcgta acggccgcgg ccagatcgac gacatcgacc acctcggcaa ccgccgcgtc
     3541 cgcgcggtcg gtgagctgat ggagaaccag taccgcatcg gcctggttcg catggagcgc
     3601 gcgatcaagg agcgcatgag catgtcccag gagatggacg cgctcatgcc ggcggacctg
     3661 atcaacgcca agccggtctc ggccgtcgtc aaggagtact tcgcctcctc gcagctgagc
     3721 cagttcatgg accagaccaa cccgctcagc gaggtcaccc acaagcgccg cctctcggcg
     3781 ctcgggccgg gtggtctcac gcgcgagcgc gccggcttcg aggtccgcga cgtgcactcg
     3841 acgcactacg ggcgcatctg cccgatcgag acgccggaag gtccgaacat cggcctgatc
     3901 gcgtcgctct cgacctacgc gcgcatcaac gagttcggct tcatcgagac cccgtaccgc
     3961 gccgtcgacg gcggcgtcgc ctcgaacgag gtccagtact acagcgcgct gcaggaggcc
     4021 ggccactaca tcgcccaggc caacgccccg atcgacgagt ccggcaagct gctcgccg
//
LOCUS       DASWNJ010001829         1591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_32394, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001829 DASWNJ010000000
VERSION     DASWNJ010001829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32394"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="VGB85_30615"
     CDS             complement(<1..1226)
                     /locus_tag="VGB85_30615"
                     /inference="COORDINATES: protein motif:HMM:NF020076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEY0138493.1"
                     /translation="MSLRTCSHLAGGLLWVACTPTHITEDQQPAPSPATPEVDQGPAP
                     APIFLPRQTLRPGDRDAGRAPVSLTASDGTGLRLVSLKARAVVEEPLAFTELHLVFEN
                     PNDRTIEGRFEIDMPPGAAISRFSMKVHGQWQEGEVVERRAAQRAYEDFLHRKQDPAL
                     LENEAGNAFSARVFPILARERKELIVAYSQDLPSSAEPYRLMLRGLPELDELDASVIL
                     HEPGVTAPGVTTSLATTTGSRRVIELRRTKWTPDQDLEVLDDRGFSMMGLRHDNLAVA
                     RVAAIGETTPAPVEGLTVLFDTSASRALGFASQVARLGEIIKSLAGAGDFELRLVAFD
                     QTTAEIYRGKASGLGEAQLAKLVEREAMGASDLGRALAAVAAADAPKLHRVLIFSDGI
                     STAGDPEITALRAGALA"
     gene            complement(1252..>1591)
                     /locus_tag="VGB85_30620"
     CDS             complement(1252..>1591)
                     /locus_tag="VGB85_30620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138494.1"
                     /translation="WETDANDVDFHVIDNTDERAYYSHKRLESGGELFADVTTGYGPE
                     CFAIRGKPSAFPYNFVAQYYSRGPMGYGMGKLQIVQHDGRGGLKFDDRPFIIMKDRAF
                     IELGKLDRPL"
BASE COUNT          228 a          503 c          615 g          245 t
ORIGIN      
        1 gcgagggcgc cggccctgag cgcggtgatc tcggggtcgc cggcggtgct gatgccgtcg
       61 gagaagatca gcacgcggtg cagcttcggc gcgtcggcgg cggcgactgc agcgagcgcg
      121 cgcccgaggt ccgaggcgcc catcgcctcg cgctcgacga gcttggccag ttgcgcctca
      181 ccgaggccgc tggccttgcc gcgatagatc tccgcggtgg tctggtcgaa ggcgacgagg
      241 cgcagctcga agtcgccggc ccccgcgagg ctcttgataa tttctccgag gcgggcgacc
      301 tgcgaggcga agccgagcgc ccgcgaggcg ctggtgtcga acagcacggt gaggccctcg
      361 accggcgcgg gcgtggtctc gccgatcgca gcgacgcggg cgaccgcgag gttgtcgtgg
      421 cgcaggccca tcatcgaaaa gccgcggtcg tcgaggacct cgaggtcctg gtcgggggtc
      481 cacttggtgc gccgcagctc gatgacgcgg cgtgaaccgg tggtggtcgc cagcgaggtg
      541 gtgacgccgg gcgcggtgac gccgggctcg tgcaagatga cgctggcgtc gagctcgtcg
      601 agctcgggca ggccgcgcaa catcaggcgg tagggctcgg cggagctcgg caggtcctgc
      661 gagtaggcga cgatcagctc cttgcgctcg cgcgcgagga tcgggaacac gcgcgcgctg
      721 aaggcgttgc cggcctcgtt ctcgagcaag gcggggtcct gcttgcggtg caggaagtcc
      781 tcgtaggccc gctgcgcggc gcggcgctcg acgacctcgc cctcctgcca ctgtccgtgc
      841 accttcatcg agaagcgcga gatcgcggcg cccggcggca tgtcgatctc gaagcggccc
      901 tcgatcgtgc gatcgttggg gttctcgaac acgaggtgca gctcggtgaa ggcgagcggc
      961 tcctcgacca cggcgcgcgc cttgagcgag acgagccgca ggccggtgcc gtccgaggcg
     1021 gtcaacgaga ccggcgcgcg gccggcgtcg cggtcgcccg ggcgcagggt ctggcgcggc
     1081 agaaagatcg gcgccggggc cgggccctgg tcgacctccg gggtcgccgg cgacggcgcg
     1141 ggctgctggt cctcggtgat gtgggtcggc gtgcaggcga cccacaacag gccgccggcg
     1201 aggtgactac atgttcgaag ggacatgtct gaggagactt tctgggcagg tctagagcgg
     1261 acggtcgagc ttgccgagct cgataaaggc gcggtccttc atgatgatga aggggcggtc
     1321 gtcgaacttg agcccaccac ggccgtcgtg ctggacgatc tgcagcttgc ccatgccgta
     1381 gcccatcggg ccgcggctgt aatactgggc gacgaagttg taaggaaacg cggacggctt
     1441 gcctcggatg gcaaagcact ccgggccgta gccggtcgtc acgtcggcga acagctcgcc
     1501 gccggactcg agccgcttgt gcgagtagta ggcgcgctcg tcggtgttgt cgatcacgtg
     1561 gaagtcgacg tcgttggcgt cggtctccca g
//
LOCUS       DASWNJ010001830         3728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_103796, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001830 DASWNJ010000000
VERSION     DASWNJ010001830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103796"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VGB85_30625"
     CDS             <1..312
                     /locus_tag="VGB85_30625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEY0138495.1"
                     /translation="RLEIEGRTFAKIKAQMLQQLSNFGQPFIYVVDANYLNRGELLLG
                     HRHEGIDLKSDYARDTLRSLERVWRRPVSLLTILDDKPKRIRFDGSEISMSDEPQAFR
                     V"
     gene            575..1348
                     /locus_tag="VGB85_30630"
     CDS             575..1348
                     /locus_tag="VGB85_30630"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEY0138496.1"
                     /translation="MANEDSFLVDASLGLYLVADGMGGHAGGAHASAMCADVVNKVVR
                     RGMARLMGMPQQTWNDAITEILQASASEASARIYDQAQLDRSLHGMGTTLTGILFHGD
                     RGYIVHVGDSRAYVLRGGTARQLTSDHSWLNEQIQAGLLTEEEAQDSDLKHIITRSVG
                     FERVVEADIVPVNVSMGDVYLLCSDGFANYVSADELATLARDNLYSELPQICTELALE
                     RGGDDNITVVVVLACNDHDERQAVRQLSKTASDTLPSLS"
     gene            1413..1794
                     /gene="rnpB"
                     /locus_tag="VGB85_30635"
     ncRNA           1413..1794
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VGB85_30635"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1811..2599)
                     /locus_tag="VGB85_30640"
     CDS             complement(1811..2599)
                     /locus_tag="VGB85_30640"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HEY0138497.1"
                     /translation="MLTIDDTSPWTIVGVDCASQDAHIGLARGTLSSAGLMLEQVRIG
                     GTRETSVAQLVASWLQGPRVLLAIDAPLGWPRTLAEALKVHRAGLPIAGEATRLMRRE
                     TDRQVAAALGKTPLDVAADRIAYAAHRALRLLGELREATGLPLPLLWEPAPGVGAIEV
                     YPAATLRACSIDASGYKNKTRAHRDTAHDARKRLLACLQTKLAISGELTSAILTAKRE
                     DMLDAAVCVLAGADFLQGHCAAPAPAELPLAQQEGWIWYRRPTT"
     gene            complement(2606..3403)
                     /locus_tag="VGB85_30645"
     CDS             complement(2606..3403)
                     /locus_tag="VGB85_30645"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEY0138498.1"
                     /translation="MTDIHPQIPALRTLQRQDRRLTSLERKLGGIPARISGLDSDLQK
                     LEQLLVAERAKLEETKNFQSRQETQLHDEEDQIRNSKAKLGQIKTARELNAQQRELET
                     TRRMVTTRTGEISKLQEAVTRTEQQIAKMEAHLSALRAAADAEKGRLAEEQTQLDESI
                     AKARKLRGKSVAAIDKEILANYERIRKRLGGLAFVPAHRERCTACKMMIPHILYTRLL
                     KGKEILPCESCGRLLYWSGHFPEDQDKFEADNKPKPAEDVIPGVPEF"
     gene            complement(3417..3490)
                     /locus_tag="VGB85_30650"
     tRNA            complement(3417..3490)
                     /locus_tag="VGB85_30650"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3454..3456),aa:Ile,seq:cat)
BASE COUNT          573 a         1246 c         1310 g          599 t
ORIGIN      
        1 cgcctcgaga tcgaaggccg cacgttcgcc aagatcaagg cgcagatgct ccagcagctg
       61 tcgaacttcg ggcagccgtt catctacgtg gtcgacgcga actacctcaa tcgcggtgag
      121 ctcctgctcg gccaccgcca cgaaggcatc gacctcaagt cggactacgc ccgcgatacc
      181 ctgcgctcgc tcgagcgagt gtggcgacgt ccggtctcgc tgctgacgat cctcgacgac
      241 aagcccaagc gcatccgctt cgacggctcg gagatctcca tgtccgatga gccgcaggcg
      301 ttccgggtgt gacgcggaag gccgtgggca agcgttggct tgctcacggc atgtggcctt
      361 tcggccacgt cgtcgctgcc aacaggcgcg tagggccatg tgacgggcct tgtgggctcg
      421 ctgtccgcgc aaaatgcgct agaagacaca cgctcgcggt ggaagacacc gcagcgaaat
      481 cgatgtgcag cacagccggc gcggtgtccg ggcgtgctaa ggtctgaccg tgcgtttgat
      541 cgcgtgggca tccaccgacg taggccgggt tcgcatggcg aacgaggaca gcttcctcgt
      601 cgacgcctca ctcgggctct acctggtcgc cgatggcatg ggcggccacg ccggcggagc
      661 ccacgccagc gcgatgtgtg ccgacgtcgt gaacaaggtc gtgcgtcgcg ggatggcccg
      721 actgatgggc atgccccagc agacctggaa cgacgcgatc acggagatcc tgcaagcgag
      781 cgccagcgag gcgtccgccc gcatctacga ccaggcccag ctcgaccgct cgctgcacgg
      841 catgggtacc accctgacgg gcatcttgtt ccacggagac cgcggctaca tcgtgcacgt
      901 cggcgactcg cgggcctacg tgctgcgcgg cggcacggcc cggcagctga ccagcgacca
      961 ctcgtggctc aacgagcaga tccaggccgg cctgctgacg gaggaggagg cgcaggacag
     1021 cgacctcaag cacatcatca cgcgcagcgt cggcttcgag cgcgtggtcg aggccgatat
     1081 cgtgccggtc aacgtcagca tgggcgacgt ctacctgctg tgctccgacg gcttcgccaa
     1141 ctacgtctcg gccgacgagc tcgcaacgct cgcccgcgac aacctgtact ccgagctgcc
     1201 ccagatctgc accgagctcg cgctcgagcg cggcggcgac gacaacatca cggtcgtcgt
     1261 cgtgctcgcg tgtaacgacc acgacgagcg ccaggccgtg cgccagctca gcaagaccgc
     1321 ctccgatacg ctgccctcgc tctcgtgatc attcttgatc attccttgat cccgcccggt
     1381 cgtgctatca cgcggccggg gagagagacg gccgaggggg acaaggcgac cgcggtgggt
     1441 gctactgctt cggcatgagc caccatcgag gaaagtccgg gctccacagg gcagggtgct
     1501 ggcgcaggcc agtcggggtg actcgaagga aagtgccaca gaaaacagac cgcccacgtc
     1561 cgcgtgggta agggtgaaaa ggtgcggtaa gagcgcaccg gggacctggc gacagggacc
     1621 gcacggtaaa ccccacccgg agcaaggcca aacagaggcg ggaggctgtc cgcaaggaca
     1681 ggtctccagg ggcggcccgc tccacctcgg gtagcgccgc tcagatgaat ggtcgccacc
     1741 cggcgaaggc aacgacgccg gggacagaac ccggcttacg accccgctcg accgtctctc
     1801 tccccacctt ttacgtcgtc ggccggcggt accagatcca gccctcctgc tgcgcgagcg
     1861 gcagctcggc gggcgctggc gcggcgcagt gcccctgtaa aaaatcggcg ccggcgagca
     1921 cgcagacggc ggcgtcgagc atgtcctcgc gcttcgccgt gaggatcgca ctcgtgagct
     1981 cgcccgagat cgcgagcttc gtctgcaagc acgcgagcag ccgcttgcgg gcgtcgtggg
     2041 cggtgtcgcg gtgggccctg gtcttgttct tgtagccgct ggcgtcgatc gagcacgcgc
     2101 gcagcgtcgc cgccgggtag acctcgatcg caccgacgcc gggcgcgggc tcccatagca
     2161 ggggcagcgg caggccggta gcctcgcgca gctcgccgag cagtcgcagg gcgcgatggg
     2221 ccgcgtaggc gatgcggtcg gcggcgacgt cgaggggcgt cttgccgagc gccgcggcga
     2281 cctggcggtc ggtctcgcgt cgcatcaggc gcgtcgcctc gccggcgatc ggcaggccgg
     2341 cgcggtgcac ttttaaagcc tccgccaggg tgcgcggcca gccgagcggc gcgtcgatcg
     2401 cgagcagcac gcgcggtcct tgcaaccacg aggcgacgag ctgcgcgacg ctggtctcgc
     2461 gggtgccacc gatgcggacc tgctcgagca tcagccccgc cgacgacagg gtgccgcggg
     2521 cgaggccgat gtgggcgtcc tgtgaggcac agtcgacccc gacgatcgtc cacggggacg
     2581 tatcgtcgat cgtgagcacc ggggcctaga actcgggcac gccggggatg acatcctcgg
     2641 cgggcttggg cttgttgtcg gcctcgaact tgtcctggtc ctccgggaag tggccggacc
     2701 agtagagcag gcggccgcac gactcgcagg gcaggatctc cttgcccttg agcaggcgcg
     2761 tgtagaggat gtgcgggatc atcatcttgc acgcggtgca gcgctcgcgg tgggcgggca
     2821 cgaaggcgag accgccgagc cgcttccgga tgcgctcgta gttggcgagg atctccttgt
     2881 cgatcgccgc caccgacttg ccgcgcagct tgcgggcctt ggcgatgctc tcgtcgagct
     2941 gggtctgctc ctcggcgagg cggcccttct cggcgtccgc cgcggcacgc agagcgctca
     3001 gatgcgcctc catcttggcg atctgctgct ccgtcctggt gacggcctcc tgcagcttcg
     3061 agatctcccc cgtgcgcgtg gtgaccatgc gccgggtcgt ctccagctcg cgctgctgag
     3121 cattgagctc gcgcgccgtc ttgatctgcc ccagcttggc cttgctgttg cggatctgat
     3181 cctcctcgtc gtggagctgg gtctcctggc gcgactggaa gttcttcgtc tcctcgagct
     3241 tggctcgctc cgccacgagc agctgctcga gcttctgcag atcgctgtcg aggccgctga
     3301 tgcgggccgg gatgccccct aacttgcgct caaggctcgt gaggcggcgg tcctgccgct
     3361 gaagcgtacg aagggctggg atctgggggt ggatatctgt catgggggta cgggggtggg
     3421 cccacctggg cttgaaccag ggaccaaccg gttatgagcc ggccgctctg accgactgag
     3481 ctatgggccc cgtcgacttg ggtcgatgtg ggcttcacga taggtagcct tgcgcagcgc
     3541 gtcaaactgc gtgacgcgcg cgggtgggcc gcgaggccat ggtggccgcg gccgcgggcc
     3601 cgccgctgtc ctggttcgtc gcatgtgcaa gcgaagccgg ggtgggccgg ccggcggcga
     3661 tcgtgcacgg gtccggccgg tctcgcggcc ggagtccgcg ggagatcggc ggcccgtggc
     3721 gcccacgt
//
LOCUS       DASWNJ010001831         3328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_454544, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001831 DASWNJ010000000
VERSION     DASWNJ010001831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454544"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1627
                     /locus_tag="VGB85_30655"
     CDS             <1..1627
                     /locus_tag="VGB85_30655"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEY0138499.1"
                     /translation="WNLYSNGEVYSTIDLPYDGKYVFRARLWGQQAGPDLPHANLTVD
                     QAPVLMIDTDAVKDAAKIYEIEITAKAGVHKFAVEFTNDYYDADLMADRNLLVDYFTV
                     EGPLDLVAGENEQRNRIMICDPVVDGEETCGRQILSAFAARAWRRPVTEEEVTRLYQF
                     ITEAKAYGLDFEAGLRVALQAVLVSPFFVFRVELDPSPTGLDPHPLTDHELASRLSYF
                     LWSSMPDEELRLAADQGLLQDPKQVEAQALRMLEDPRAAALIDNFAGQWWLIRNVNIA
                     FKDVLLYPQWDDGMKESMRTELRLFAESFFNSDRSMLEMLTSTKTFVNDRLAAHYGIE
                     GAFGPEFVEADFGDRPYQGILNKAGLMTVLSHADHTSVVKRGKWVMENLLCVTPPPPP
                     PGVDTKLEPTVGKTQREILEQHRKDPKCAGCHVVMDPLGFGLEHYDPLGAWREDENGL
                     TLDPSGTLPNGKPFADGLEMQTLISSEPEFSKCVARKTFIYALGRGTTLGDLDYLEEV
                     VAGFEASQYRFADLVVNLVTSDVFRQRRGDPSM"
     gene            1667..3043
                     /locus_tag="VGB85_30660"
     CDS             1667..3043
                     /locus_tag="VGB85_30660"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEY0138500.1"
                     /translation="MKRNPHMKRNLPLSRRTMLGGAGALIALPWLEALAPRVGGRANA
                     APEPSPKRALFYYLPCGIHMQSWTPATEGPNYELTKILSPLVDQNAMRDLKGDTMVIT
                     GLANYPAKPEGPGDHAGGTSAFLTCTHVLKSETDFLNDISIDQLIANAIGDATPHRSL
                     QFGTAGGGTMGNCDSGYSCAYTSNISWADPKTPLPKLSAPQVIFELLFSGFDPSATAE
                     QLARRRANRLSVIDYANDQVLALQQKLGKTDRDKLEQYLTGLRDLEKRVTDEKTEAIC
                     ELPAMFSGAFQDHQTHVDLMTELMVLAFQCDQTRVITFMLENAGSGRDYAFIGAPGGH
                     HEISHHQSLQTNYDKLEIIDQWEVAQFAKLLQKLKDSPDGDGTLLDNCAVYLSSEISD
                     GNQHNHDNMPVLLAGGLGGAITPGRHLKVPDKTPLANLFASIATGMGVPTGVFGDDGT
                     GLLDGLKL"
BASE COUNT          573 a         1263 c         1007 g          485 t
ORIGIN      
        1 ctggaacttg tactccaacg gcgaggtcta ctcgacgatc gatctgcctt acgacggcaa
       61 gtacgtgttc cgcgcgcgcc tgtggggcca gcaggccggc cccgacctgc cgcacgccaa
      121 cctgaccgtc gaccaggccc ccgtcctgat gatcgacacc gacgcggtca aggacgccgc
      181 caagatctac gagatcgaga tcaccgccaa ggccggcgtg cacaagttcg cggtcgagtt
      241 caccaacgac tactacgacg ccgacctgat ggccgaccgc aacctgctgg tcgactactt
      301 caccgtcgag ggtccgctcg acctcgtcgc gggcgagaac gagcagcgca accgcatcat
      361 gatctgcgac cccgtcgtcg acggcgagga gacgtgtggc cgccagatcc tgtccgcctt
      421 cgccgctcgc gcgtggcgtc gcccggtcac cgaggaggag gtcacgcgcc tctaccagtt
      481 catcaccgag gccaaggcct acggcctgga cttcgaggcc ggcctgcgcg tcgccctgca
      541 ggcggtcctc gtctcgccct tcttcgtgtt ccgcgtcgag ctcgacccga gcccgaccgg
      601 cctcgacccg cacccgctga ccgaccacga gctcgcctcg cgcctgtcct acttcctgtg
      661 gagcagcatg cccgacgagg agctgcgcct cgcggccgac cagggcctgc tgcaagaccc
      721 caagcaggtc gaggcgcagg cgctgcgcat gctcgaggac ccgcgcgccg ccgcgctgat
      781 cgacaacttc gccggtcagt ggtggctgat ccgcaacgtc aacatcgcct tcaaggacgt
      841 gctgctctac ccgcagtggg acgacggcat gaaggagtcg atgcgcaccg agctgcgcct
      901 gttcgccgag agcttcttca acagcgaccg cagcatgctc gagatgctga cgagcaccaa
      961 gaccttcgtc aacgaccgcc tcgcggccca ctacggcatc gagggcgcgt tcgggcccga
     1021 gttcgtcgag gccgacttcg gcgaccgccc gtaccagggc atcctcaaca aggccggcct
     1081 gatgaccgtg ctctcgcacg ccgaccacac ctcggtggtc aagcgcggca agtgggtgat
     1141 ggagaacctg ctgtgcgtca cgccgccgcc gccgcccccg ggcgtcgaca ccaagctcga
     1201 gccgaccgtc ggcaagaccc agcgcgagat cctcgagcag caccgcaagg accccaagtg
     1261 cgccggctgc cacgtcgtca tggacccgct cggcttcggg ctcgagcact acgatccgct
     1321 cggcgcctgg cgcgaggacg agaacggcct gacgctcgac ccgtcgggca cgctgcccaa
     1381 cggcaagccc ttcgccgacg gcctcgagat gcagacgctg atcagcagcg agcccgagtt
     1441 tagcaagtgc gtcgcgcgca agaccttcat ctacgcgctc ggtcgcggca cgacgctcgg
     1501 cgacctcgac tacctcgagg aggtcgtcgc cggcttcgaa gccagccagt atcgcttcgc
     1561 cgacctcgtc gtcaatctcg tcaccagcga tgtgttccgc cagcgccgcg gcgacccgtc
     1621 catgtgagcc acccagtccc gcgagtcgtc caggagaacc cagatcatga agcgcaatcc
     1681 ccatatgaag cgcaacttgc cgctctctcg tcgcacgatg ctcggtggcg ccggcgccct
     1741 gatcgccctc ccgtggctcg aggcgctcgc gcctcgcgtc ggcggtcgcg ccaacgcggc
     1801 cccggagccc tcgcccaagc gcgccctgtt ctattacctg ccctgcggga tccacatgca
     1861 gtcgtggacg ccggccaccg agggcccgaa ctacgagctg accaagatcc tgtcgccgct
     1921 cgtcgatcaa aacgcgatgc gcgacctcaa gggtgacacg atggtcatca ccggcctggc
     1981 gaactacccc gccaagccgg aggggccggg cgaccacgcc ggcggcacct cggcgttcct
     2041 gacctgcacc cacgtgctca agtcggagac cgacttcctc aacgacatct cgatcgacca
     2101 gctgatcgcc aacgcgatcg gcgacgcgac cccgcaccgc tcgttgcagt tcggcaccgc
     2161 cggcggcggc acgatgggca actgcgacag cggttactcg tgcgcctaca ccagcaacat
     2221 ctcgtgggcc gacccgaaga ccccgctccc caagctctcc gcgccgcagg tgatcttcga
     2281 gctgctgttc tccggcttcg acccgagcgc gaccgccgag cagctggccc gccgccgcgc
     2341 caaccgcctg agcgtcatcg actacgccaa cgaccaggtg ctcgcgctgc agcagaagct
     2401 cggcaagacc gaccgcgaca agctcgagca gtacctcacc ggcctgcgcg acctcgagaa
     2461 gcgcgtcacc gacgaaaaaa ccgaggccat ctgcgagctg ccggcgatgt tctccggcgc
     2521 cttccaggac caccagaccc acgtcgatct gatgaccgag ctgatggtcc tcgcgttcca
     2581 gtgcgaccag acccgcgtga tcaccttcat gctcgagaac gccggcagcg gccgcgacta
     2641 cgcgttcatc ggggcgcccg gcggtcacca cgagatcagc caccaccaga gcctgcagac
     2701 caactacgac aagctcgaga tcatcgacca atgggaggtc gcgcagttcg ccaagctgct
     2761 gcaaaagctc aaggacagcc ccgacggcga cggcaccctg ctcgacaact gcgccgtgta
     2821 cctgagctcc gagatctctg acggcaacca gcacaaccac gacaacatgc ccgtgctgct
     2881 cgccggcggg ctcggcggcg cgatcacgcc ggggcgccac ctcaaggtcc ccgacaagac
     2941 cccgctcgcc aacctgttcg cgtcgatcgc caccggcatg ggcgtgccga ccggcgtgtt
     3001 cggcgacgac ggcaccggcc tgctcgacgg cctcaagctc tgagcgacga gcaccacgga
     3061 acccatctgt cccgcgggac aggtgggttc gctcgtcggt gcgaccggcg cgcgtgcccg
     3121 cgcctcgttc ggggtttttt ctcgccgcag gccggtcccg cgaaccgcct tcccagcgag
     3181 ccacgcgcct gatcccgggc ggaacagcgg ccttggatcg ggcgtgctaa ggtcgctgcg
     3241 tggccgaggc accctccccc ttcgccctcc tgctcggcgc cgcgttcgcc gcggcctcgg
     3301 gcttcgccat ggccaacctg tggctgcg
//
LOCUS       DASWNJ010001832         2095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_136965, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001832 DASWNJ010000000
VERSION     DASWNJ010001832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136965"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="VGB85_30665"
     CDS             <1..1187
                     /locus_tag="VGB85_30665"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY0138501.1"
                     /translation="VLAVVELPAEGPALDLSHLTASARGLDVIWVILESTGARHLKSY
                     GATLDATPRLTGLAQQALIFERMYTSYPESIKSLLPILCGAAPALHTPAHAYAAGRSP
                     CEALPVALAAAGYRTGLFHSGRFVYLGMADMVEGRGFDVLADAAVIGGPHAVSFGTDD
                     RSTVRRVLQFIDEAGDRPYFALYMPIAGHHPYRTPGTGTRPFPATSEQEHHRNDLYVG
                     DEALGELIDGVRARGRMDRTLWIVMGDHGEAFHEHPANFAHSLFVYEENVHIPVIMVV
                     PGALRQALRVPQVGGTIDLAPTLLDLLGLGRIGAGRSLLRGEPGVARFFTDHSGVLLG
                     LRHDRWKCIVDVERGTTRLFDLEADPGETEDVAAREPARAQRYQVALAAWAAGNAARL
                     GR"
     gene            complement(1189..2013)
                     /gene="asd"
                     /locus_tag="VGB85_30670"
     CDS             complement(1189..2013)
                     /gene="asd"
                     /locus_tag="VGB85_30670"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF014696.4"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HEY0138502.1"
                     /translation="MPRLHRSAAFLRMYWAAPHALLGRITRALATATAPRWAIDRVIA
                     RWIRRAAIDMHDFEAGPFPTLEAFFLRRLRPGARPIADGFVAPADGIVVGAGPIDHGT
                     IVQVKGIDLDLARLTCGRGPALPLAAYEGGRYTTIFLTPDGYHHVHMPVAATVHELRW
                     IPGRAFPQNHDALRTIPRIYERNERVTLRCTTDDGHDFLLIMVGASLIGGIHLDGLER
                     AAWARRLPFHWRRSFARGERLGHFSFGSTVVLLVPPALAGPTPAIGEPLRMGQRLW"
BASE COUNT          291 a          645 c          866 g          293 t
ORIGIN      
        1 aggtgctcgc ggtcgtggag ctgcccgcgg agggaccggc gctcgacctg tcgcacttga
       61 ccgcgtcggc gcgcggcctc gacgtgatct gggtgatcct cgagtcgacc ggcgcgcggc
      121 atttaaaaag ttacggggcg acgctcgacg cgacgccgcg gctgacgggg ctggcccagc
      181 aggcgctgat cttcgagcgc atgtacacga gctatcccga gagcatcaag agcctgctgc
      241 cgatcctgtg cggggccgcc ccggcgctgc acaccccggc gcacgcgtat gcggcggggc
      301 gatcgccgtg tgaggcgttg ccggtggcgc tcgccgccgc gggctaccgc acggggttgt
      361 tccactcggg gcgcttcgtg tacctcggga tggcggacat ggtcgagggc cgcggcttcg
      421 acgtgctcgc ggacgcggcg gtgatcggtg ggccgcacgc ggtcagcttc gggacggacg
      481 accgctcgac ggtgcgacgg gtgttgcagt tcatcgacga ggccggcgat cggccgtact
      541 tcgcgctgta catgccgatc gcggggcatc acccgtatcg cacgccgggc accgggacgc
      601 ggccgttccc ggcgacgagc gagcaggaac atcaccgcaa cgatctatat gtcggcgacg
      661 aggcgctcgg cgagctgatc gacggggtgc gcgcgcgcgg acgcatggat cgcaccctgt
      721 ggatcgtgat gggggaccac ggcgaggcgt tccacgagca tcctgccaac tttgcccact
      781 cgctgttcgt gtacgaggag aacgtgcaca tcccggtgat catggtcgtg cccggggcgc
      841 tgcggcaggc gctacgggtc ccgcaggtcg gaggcacgat cgacctggcg ccgacgctgc
      901 tcgacctgct cgggctggga aggatcgggg cgggtcgatc gctgctgcgc ggcgagccgg
      961 gggtcgcacg cttctttacc gaccacagcg gcgtgctgct cgggctgcgc cacgatcgct
     1021 ggaagtgcat cgtcgatgtc gagcgtggga cgacgcggct gttcgacctc gaggcggatc
     1081 cgggcgagac ggaggatgtc gcggcgcgcg agccggcgcg ggcgcagcgg tatcaggtcg
     1141 cgctcgcggc gtgggcggcc ggcaacgcgg cgcggctcgg gcggtgattc accacaggcg
     1201 ctggcccatg cgcaggggct cgccgatcgc gggcgtgggg ccggcgagcg cgggcggcac
     1261 gagcagcacg acggtcgagc cgaagctgaa gtgaccgagg cgctcgccgc gggcgaagct
     1321 gcggcgccag tgaaacggca ggcggcgggc ccaggcggcg cgctcgaggc cgtcgaggtg
     1381 gatgccgccg atcaggctgg cgccgaccat gatcaacagg aagtcgtggc cgtcgtcggt
     1441 ggtgcagcgc agggtgactc gctcgttgcg ctcgtaaatt cgcgggatcg tgcgcagggc
     1501 gtcgtggttc tgcgggaacg cgcggccggg gatccagcgc agctcgtgca cggtcgcggc
     1561 gaccggcatg tgcacgtggt ggtaaccatc gggggtcagg aagatcgtcg tgtagcggcc
     1621 gccctcgtag gcggcgagcg gcagcgcggg gccgcggccg caggtgaggc gggcgaggtc
     1681 gaggtcgatg cccttgacct gcacgatcgt gccgtggtcg atcgggccgg cgccgacgac
     1741 gatgccgtcg gcgggggcga cgaagccgtc ggcgatcggg cgggcgccgg gtcggaggcg
     1801 gcgcaggaag aacgcctcga gggtcgggaa ggggccggcc tcgaagtcgt gcatgtcgat
     1861 cgcggcgcga cggatccagc gcgcgatgac gcggtcgatc gcccagcggg gggcggtggc
     1921 ggtggcgagg gcccgcgtga tccggccgag cagggcgtgc ggggcggccc agtacatgcg
     1981 caggaaggcg gcgctgcggt gcaggcgagg cacggggcga ggttaacatg ggtccgcggg
     2041 aatatcggcg ggggggcatg gttgctcgtc gtcgcgggat gcggacgatg cacgg
//
LOCUS       DASWNJ010001833         3513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_668653, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001833 DASWNJ010000000
VERSION     DASWNJ010001833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668653"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VGB85_30675"
     CDS             complement(<1..837)
                     /locus_tag="VGB85_30675"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0138503.1"
                     /translation="MSEQRTSRSAPTKIPAGAPIPVEEDAPGPAYSIRDVSRLFDLPE
                     SRLRYWSQTGFIQPSIRVKGRTWYSFRDLIGIKVARELLGAGLSLQRVRRSLDALRVK
                     LPEVDSPLSRLRVRCEHDRVIVEGSDRSYEAATGQGLLDFSVQTLQQQASQVLALPRV
                     HDDGDAEDRSAYEWFTIACERERSWDGQDPDDPAFLDARQAYDRALDLDPEFAAAYTN
                     LGALLAAVGDLDGARDHFDQALRCDPDQPEAQSNLAALALRLGDHDTAIAGYRQLLRA
                     SPD"
     gene            complement(830..1327)
                     /locus_tag="VGB85_30680"
     CDS             complement(830..1327)
                     /locus_tag="VGB85_30680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138504.1"
                     /translation="MGTPPPSYKTLAGLLPEARWARVHKVQLIRREIRETLSPSALEV
                     LWRYGEHVVEGGGEVGGKGPGSRFYATVMVTFDLRRCAAYFREPADVATAERLAELMV
                     GSPRVQKKLVELARPELARLAQVPGDRLRIELDHHARAEGTQVLIDGDAMVSLEPARK
                     AGTHE"
     gene            1513..2496
                     /gene="gshB"
                     /locus_tag="VGB85_30685"
     CDS             1513..2496
                     /gene="gshB"
                     /locus_tag="VGB85_30685"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF003573.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEY0138505.1"
                     /translation="MTAEPTTPPAHGDVLFVLDPLSTIHPQKDSSYLMIAEAQRRGFQ
                     PWAVELSGLMLRGADAFAYATPISVPRLGQPVVVRGPSEIRDLASYRVVLMRKDPPVD
                     SHFIVATWALDRAMHKTLVVNTPQGLRDLSEKLAILEFPQFIPRSFLLRNIGDLRDAL
                     NELGGQMILKPVYGYGGREVLIARDGDPNLTTLFEIATRDGTTWTIAQAFVPEAKNGD
                     KRILMVDGEPIGAVMRVPASGEIRNNFHAGGSPAVTELSARDLQICAAVGPFVRSRGQ
                     FFVGLDVLGDYLTEINVTSPTGMQEINRLENLTGESTMQAKFWSALETKLG"
     gene            2621..3475
                     /locus_tag="VGB85_30690"
     CDS             2621..3475
                     /locus_tag="VGB85_30690"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEY0138506.1"
                     /translation="MSGSRTEAFERLRELTGLPNALLIFQSPRPGDALLGLLARHIEE
                     FLGRKAEKSFQILDDILRDDITHPIDINTRGIDGDFGRVHPLLWELKRTCLTSVLGCL
                     PPGVRISFILTDLLGYSPAASAALLGINESAFRVRLTRARKRLDNYLAPLCQFVDKAN
                     PCNCEGRLGVALAKEFISFPPHTDDIPHAPHDGGPEFHEMGALYRALPAIALTEEQRN
                     KLLTACPEEEEHLVNIDDLEDPQDIVLAGPVEPDHPAGPDEPAQPDDPIAPGPEPIHI
                     PADPAAHG"
BASE COUNT          544 a         1308 c         1123 g          538 t
ORIGIN      
        1 atccggcgag gcacgcagca gctgccggta ccccgcgatc gccgtgtcgt gatcgccgag
       61 gcgcagggcc agcgcggcga ggttgctctg cgcctccggc tggtccgggt cgcagcgcag
      121 cgcctggtcg aagtggtcgc gcgcgccgtc gaggtcgccg accgcggcga gcagcgcccc
      181 caggttcgtg taagccgccg cgaactcggg gtcgaggtcg agggcgcggt cgtaggcctg
      241 gcgcgcgtcg aggaacgccg ggtcgtcggg gtcctggccg tcccagctgc gctcgcgctc
      301 gcaggcgatc gtgaaccact cgtaggcgct gcggtcctcc gcgtcgccgt cgtcgtggac
      361 ccgcgggagc gccagcacct gcgatgcttg ctgctgcaag gtctggacgc tgaagtcgag
      421 caggccctgg ccggtcgccg cctcgtagct gcggtcgctg ccctcgacga tcacgcggtc
      481 gtgctcgcag cggacgcgca ggcgcgacag cggcgagtcg acctcgggca gcttgacgcg
      541 caacgcgtcg aggctgcggc gcacgcgttg caacgacagc ccggcgccga gcagctcacg
      601 cgccaccttg atgcctatca ggtcgcggaa gctgtaccag gtgcggccct tgacgcggat
      661 gctcggctgg atgaagcccg tctgcgacca gtagcgcagg cggctctccg gcaggtcgaa
      721 cagccgcgag acgtcgcgga tcgagtaggc cgggcccggc gcgtcttcct ccacggggat
      781 cggcgcgccg gcaggaattt tggtcggcgc actgcgcgag gtccgttgct cactcatggg
      841 tccctgcctt gcgtgcgggc tccagcgata ccatggcgtc gccgtcgatg agtacctgcg
      901 tgccctccgc gcgcgcgtga tgatcgagct cgatgcgcag gcgatcgccc ggcacctgcg
      961 cgagtcgtgc caactccggc cgagccagct cgaccaattt cttctgcact cgcgggctgc
     1021 cgaccatcag ctcggccagc cgctccgccg tggcgacgtc cgccggctcg cgaaagtagg
     1081 ccgcgcagcg ccgcaggtcg aaggtcacca tcaccgtcgc gtagaagcgg ctccccggtc
     1141 ccttgcctcc gacctccccg cccccctcga ccacgtgctc gccgtaccgc cacagcacct
     1201 cgagcgcgct cggcgacagc gtctcccgga tctcccgccg gatgagctgg accttgtgga
     1261 cccgcgccca gcgagcctcc ggcagcagcc cggcgagcgt cttgtaagaa ggagggggtg
     1321 tacccacagg acctacaggt atggcatcga cttacccaag gcccaaattt cgcgcacgca
     1381 tccagcgccg gtctgcgcga cctgctgtgc ggtctttgcg gcgctccatc ttttgcatca
     1441 cagcggcgcc ggccgcgggc gaggcgcgac agcgccccgc cggcggcgct cgaccgggta
     1501 tgctgcggcc cgatgaccgc cgagcccacg accccgcctg cccacggcga cgtactgttc
     1561 gtcctcgacc cgctctcgac gatccacccg caaaaggaca gctcctacct gatgatcgcc
     1621 gaggcccagc gccgcggctt tcagccgtgg gcggtcgagc tctccggcct gatgttgcgc
     1681 ggcgccgacg ccttcgccta cgccacgccg atcagcgttc cgcggctcgg tcagcccgtc
     1741 gtcgtccgcg gcccctccga gatccgcgac ctcgcgagct accgcgtcgt gctgatgcgc
     1801 aaggacccgc cggtcgactc gcacttcatc gtcgcgacct gggccctcga ccgcgcgatg
     1861 cacaagaccc tcgtcgtcaa cacgcctcag ggcctgcgag atctcagcga gaagctcgcc
     1921 atcctcgaat tcccgcaatt catcccccgc agtttcttgc tgagaaatat tggtgatctg
     1981 cgtgacgctt taaacgagct gggcggccag atgatcctca agccggtgta cggctacggc
     2041 ggccgcgagg tgctcatcgc gcgggacggc gacccgaacc tgacgaccct cttcgagatc
     2101 gcgacccgcg acggcacgac ctggacgatc gcccaggcct tcgttcctga ggcaaaaaac
     2161 ggcgataaac gcatcctgat ggtggatggc gagccgatcg gtgcggtgat gcgggtcccg
     2221 gcctcgggtg agatccgcaa caacttccat gcgggcggct cgcccgcggt caccgagctc
     2281 agcgcgaggg acctccagat ctgcgcggcg gtcgggcctt ttgtccgcag tcgagggcag
     2341 ttcttcgtcg gcctcgacgt gctcggcgat tacctgactg aaatcaatgt aacgagcccc
     2401 acaggaatgc aggagatcaa tcgcctggag aacctcacag gtgagtccac gatgcaggcg
     2461 aaattctgga gcgccctcga gaccaagctg ggctgaccgg agcacgcgac tgcaaatggt
     2521 gctagaccag acgtcggccc tcgagatgca aggtcccccg cccactcttc gccaagtctt
     2581 cgccgccgag ctgccgcgac tctacacggt cgcgtaccac atgagcggct cgcggacgga
     2641 ggccttcgag cgcctgcggg agctcaccgg cctgccgaac gcgctcctga tctttcagtc
     2701 gccgcgtccg ggggacgcgc tgctcggcct gctcgcgcgt cacatcgagg agttcctcgg
     2761 tcgcaaggcc gagaagtcgt tccagatcct cgacgacatc ctgcgcgacg acatcacgca
     2821 cccgatcgac atcaacaccc gcggcatcga cggcgacttc ggtcgcgtgc acccgctgtt
     2881 gtgggagctc aagcgcacct gcctgacctc cgtgctcggc tgcctcccgc ccggcgtgcg
     2941 tatctccttc atcctgacgg acctccttgg ttacagcccc gccgcctcgg ccgcgctgct
     3001 cggcatcaac gagtccgcct ttcgcgtgcg cctgacccgc gcccgcaagc gcctcgacaa
     3061 ctacctcgcc ccgctgtgcc agttcgtcga caaggcgaac ccgtgcaact gcgagggccg
     3121 cctcggcgtc gcgctggcga aggagttcat cagcttcccg ccgcacaccg acgacatccc
     3181 gcacgccccg cacgacggcg gccccgagtt ccacgagatg ggcgccctct accgcgcgct
     3241 ccctgcgatc gcactgacgg aggagcagcg caacaagctg ctcacggcct gccccgagga
     3301 agaggagcac ctggtgaaca tcgacgacct tgaagacccg caagacatcg ttttggccgg
     3361 ccccgtcgag cccgaccacc ccgccggccc cgacgagccg gcccagcccg acgacccgat
     3421 cgcccccggc cccgagccga tccacatccc ggccgacccg gccgcgcacg gctgagctgt
     3481 ccgaagccgc agcgccgacc ccgatgcacc ggg
//
LOCUS       DASWNJ010001834         1965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_526389, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001834 DASWNJ010000000
VERSION     DASWNJ010001834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526389"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VGB85_30695"
     CDS             <1..639
                     /locus_tag="VGB85_30695"
                     /inference="COORDINATES: protein motif:HMM:NF013821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HEY0138507.1"
                     /translation="GAGRRGGVHELAELLRPAGITVDVVEAVPVPGDMSAAPDKLSSS
                     GIRAAVVAGEVTTAHAMLGRWYSVEGSVVRGAGRGRELGVRTANVDAGSALLPRPGIY
                     AGALGVVGGPGPWPAAISLGKNPTFVNDPDAPLVLEVHALDQQLGDSLYGATVEVAFA
                     ARLRDEQRFPGPAELMAQISRDLAAVRPALDPAALATFATHLSFATHLSPAT"
     gene            636..1094
                     /locus_tag="VGB85_30700"
     CDS             636..1094
                     /locus_tag="VGB85_30700"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0138508.1"
                     /translation="MTPKPAAPALVSATLAQVYIQQDHLEQARRMLARVLQTNPEHGH
                     ALALQARLQARSRARLYAGYRPGRGLAVRWHDAPDLPDLHLLAAIFRPVPGISGASLY
                     VTSTACRSTSGEHSFTFSGDARGPATATLCLARWDGARLLPVAVHEPLSW"
     gene            1109..>1965
                     /gene="dapA"
                     /locus_tag="VGB85_30705"
     CDS             1109..>1965
                     /gene="dapA"
                     /locus_tag="VGB85_30705"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEY0138509.1"
                     /translation="MFRGAYTALVTPLRNDALDEQAIVRLAEQQIAGGIDGLVPCGTT
                     GEASTLTLREHVRVVELTVQAAAGRVPVLAGAAANDTRKAIELARACKQVGAAGTLQV
                     TPYYNRPTQPGLVAHFTAIAEASDLPVVVYNVPGRTGVDLLPETVATLARHPRIVGIK
                     EATGDMVRIARIRELLGDSDGDGSRFTLLSGDDFTLLPFLALGGHGVISVTSNVAPRW
                     ISALCDAAAAGQWDEARTWHYRQLPLCRAMFGQPNPIPVKSALALLGRCAPDMRLPLI
                     AIDPQSREG"
BASE COUNT          255 a          841 c          622 g          247 t
ORIGIN      
        1 ggcgccggcc gccgcggcgg cgtccacgag ctcgccgagc tgctgcgccc ggccggcatc
       61 accgtcgatg tcgtcgaggc cgtgcctgtc cccggggaca tgtccgccgc gccagacaaa
      121 ctgagcagct cggggatccg cgccgccgtc gtcgccgggg aagtgacgac cgcccacgcc
      181 atgctcggcc gctggtacag cgtcgagggc agcgtcgtgc gcggggcggg ccggggccgc
      241 gagctcggcg tgcgcacggc caacgtcgac gccggcagcg cactcctgcc gcgtccggga
      301 atctacgccg gggcgctggg cgtcgtcggc ggacccggac catggcccgc cgcgatcagc
      361 ctcggaaaga accccacctt cgtgaacgac cccgacgccc cgctcgtgct cgaggtccac
      421 gccctcgacc aacagctcgg cgacagcctc tacggcgcca ccgtcgaggt ggccttcgcc
      481 gcccgcctgc gcgacgagca gcgcttcccc ggccccgccg agctcatggc ccagatctcg
      541 cgcgacctcg ccgcggtccg ccccgcgctc gaccccgccg cgctcgccac cttcgccacg
      601 cacctgtctt tcgcgacaca cctgtccccc gcgacatgac cccaaagcca gccgcacccg
      661 cgctcgtcag cgccaccctc gcccaggtgt acatccagca ggaccacctc gagcaggcgc
      721 gtcgcatgct cgcgcgggtg ctgcagacca accccgagca cggccacgcg ctggcgctgc
      781 aggcccgcct gcaggcccgc tcgcgcgccc gcctgtacgc cggctaccgc ccgggccgag
      841 gcctcgccgt ccgctggcac gacgcccccg acctccccga ccttcacttg ctcgcggcga
      901 tcttccgccc ggtccccggg atctccggcg cctcgctgta cgtcacctcc accgcctgcc
      961 gcagcaccag cggcgagcac agcttcacct tctccggcga cgcacggggc cccgcgaccg
     1021 cgaccctctg cctcgcccgc tgggacggcg cccgcctgct cccggtggcc gtccacgaac
     1081 ccctgtcatg gtaaggtcgg actcgcccat gttccgcgga gcctacaccg ccctcgtcac
     1141 gcctctccgt aacgacgccc tcgacgagca agcgatcgtc cgcctcgccg agcagcagat
     1201 cgccggcggc atcgacggcc tggtcccctg cggcaccacc ggcgaggcga gcacgctgac
     1261 gttgcgcgag cacgtgcgcg tcgtcgagct gaccgtccag gcggccgccg gccgcgtgcc
     1321 ggtgctcgcc ggcgcggccg ccaacgacac ccggaaggcg atcgagctcg cccgcgcgtg
     1381 caagcaggtc ggcgccgccg gcacgctgca ggtcacgccc tactacaacc gcccgaccca
     1441 gcccggcctc gtcgcccact tcaccgcgat cgccgaggcg agcgacctcc cggtcgtcgt
     1501 ctacaacgtg cccggccgca ccggcgtcga cctgctgccc gagaccgtgg cgaccctcgc
     1561 ccgtcacccg cggatcgtcg gcatcaagga ggcgaccggc gacatggtcc gcatcgcgcg
     1621 gatccgcgag ctgctcggcg acagcgacgg cgacggcagt cgctttaccc tgctctcggg
     1681 cgacgacttc acgctgctgc ccttcctcgc gctcggcggt cacggcgtca tctccgtgac
     1741 cagcaacgtc gcgccgcgct ggatctccgc gctgtgcgac gccgcggccg ccggccagtg
     1801 ggacgaggcc cgcacctggc actaccgcca gctgccgctg tgccgcgcga tgttcggcca
     1861 gcccaacccg atccccgtca agtcggcgct ggccctgctc ggccgctgcg cgcccgacat
     1921 gcgcctgcct ctgatcgcga tcgaccccca gagccgcgag ggcca
//
LOCUS       DASWNJ010001835         1599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_103889, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001835 DASWNJ010000000
VERSION     DASWNJ010001835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103889"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..1366
                     /locus_tag="VGB85_30710"
     CDS             203..1366
                     /locus_tag="VGB85_30710"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138510.1"
                     /translation="MRLDDLRRLLKLLDDASGPQDVFMTLRDRSMPADARDRALGAEY
                     RRLRELANPERFMGNPPAQDLAREILTRLEALYQAAPGAVDDPAGTPPSEPPRAAVVE
                     PPRASEPPRDGGSFTVIAGANHYRLDTRVTIGELAAIHRGRCVAGPHAGTEVAAKVAL
                     DRGDDPLILAEIQALQLLAHGEASQRSQVPALLDVFFKSGPCGHSGVLLRWLDGLDGA
                     SLRARFTAGVPPEHVIWIGRRLLSVTGYAHKLGVIHANIEPAHVIVRPHDHHVTLIDW
                     CYSVIDPARTHQRFRVYNPAYSAPEVAESKPPLPAADLYSIGRCMLFLLGGDLATGAV
                     PDAVPDRLRRFVAYLTRPSPLQRPQDAFEMFHELDRTRAQIYGAHRFVELVVP"
     gene            complement(1370..>1599)
                     /locus_tag="VGB85_30715"
     CDS             complement(1370..>1599)
                     /locus_tag="VGB85_30715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HEY0138511.1"
                     /translation="SDTTGTPATTGAPGETGDGPTTGAGSTSSDSGDSTGTPQAMTPE
                     SSGCSCDAPSTDPRGTFALLALVFGLARRRR"
BASE COUNT          214 a          616 c          541 g          228 t
ORIGIN      
        1 ccgagcccgt tgcccgctcc cgtgatgagg gcgaccttac cgtcgtatcg aagcgcgtct
       61 gtcatggtgg catcatgggc atatcgagag cgcgcggacg cgtcaacggc ctcgcgtccg
      121 cgcgaccagg tccggccgcc ggggcttgac gctcgatcgg cgctgccgct aagctggcgg
      181 tcgaagtcgg agcccgccgt agatgcgtct cgatgacctg cggcggttgc tcaagctgct
      241 cgacgacgcg tccggcccgc aggacgtgtt catgaccctg cgcgaccgca gcatgccggc
      301 cgacgcccgc gaccgggcgc tcggcgccga atatcgccgc ctgcgcgagc tcgcaaaccc
      361 cgaacgcttc atggggaatc cccccgccca ggacctcgcc cgcgagatcc tcacccgcct
      421 ggaggcgctc taccaggccg cgcccggggc cgtcgacgac ccggccggca cgcccccgag
      481 cgagcccccg cgcgccgcgg tcgtcgaacc cccgcgcgcc agcgagcccc cgcgcgacgg
      541 cgggagcttc accgtgatcg cgggcgccaa ccactatcgc ctcgatacac gcgtgacgat
      601 cggcgagctc gcggcgatcc accgcggtcg ctgcgtcgcg gggcctcacg ccggcaccga
      661 ggtcgcggcc aaggtggcgc tcgaccgtgg cgacgacccg ctgatcctcg ccgagatcca
      721 ggcgctgcaa ctgctcgccc acggcgaggc cagccagcgc tcgcaggtgc ccgcgctgct
      781 cgacgtgttc ttcaagagtg gcccctgcgg ccactcgggc gtgctgctgc ggtggctcga
      841 cggcctcgac ggcgccagcc tgcgcgcgcg cttcaccgcc ggggtcccac cagagcacgt
      901 catatggatc ggccgccgcc tgctcagcgt caccggctac gcgcacaagc tcggggtgat
      961 ccacgccaac atcgagccgg cgcacgtgat cgtgcgaccg cacgaccacc acgtcacgct
     1021 gatcgactgg tgttacagcg tcatcgatcc ggcgcgcacg caccagcgct ttcgcgtgta
     1081 caaccccgcg tacagcgcgc ccgaggtcgc cgagagcaag ccgccgctgc cggccgccga
     1141 cctgtactcg atcggccgct gcatgctgtt tttgctcggt ggcgacctcg cgaccggagc
     1201 ggtgcccgac gcggtgcccg accgcctgcg ccgcttcgtc gcctatctta cgcgcccgag
     1261 cccgctgcag cggccccagg acgccttcga gatgttccac gagctcgacc gcacaagggc
     1321 ccagatctac ggcgcgcacc gcttcgtcga gctcgtggtc ccgtgacgct cagcgacgac
     1381 gtcgcgcgag gccgaatacg agggcgagca gcgcgaacgt gccgcgtgga tcggtggagg
     1441 gcgcgtcgca gctgcaaccc gagctctccg gcgtcatggc ctgcggtgtg ccggtgctgt
     1501 ctccgctgtc gctgctcgtc gaccctgcgc ccgtggtcgg gccgtcgccg gtctccccgg
     1561 gcgcgccggt ggtggccggc gtgccagtgg tgtcggacg
//
LOCUS       DASWNJ010001836         1991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_415371, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001836 DASWNJ010000000
VERSION     DASWNJ010001836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415371"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VGB85_30720"
     CDS             <1..349
                     /locus_tag="VGB85_30720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138512.1"
                     /translation="DPALATVADWLRHDYGVERWGTIVSHLAFSLHLLHHHLAGRPAA
                     FVYTELYMQILRSLRPGGVFAYAPGLPFLERLLPRGLYQCTRLTAPPGPGLRAAAADT
                     GLQLGHATQVRRL"
     gene            618..1184
                     /locus_tag="VGB85_30725"
     CDS             618..1184
                     /locus_tag="VGB85_30725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138513.1"
                     /translation="MRAYQDCPGCACLVGAHEAACPFCGQALRSVTASGWLTLGLVLS
                     LGTVSISCGDKDGETDDTVTASNSDSQGPDSNGDVTDTNPYPDGVTYAGPDESSVSDP
                     YASTSDSSDPNTTPDSSPDGSTYAGPDENSTTTGDESSGSTGADTEGSSGSTGDTEGG
                     SGSTTTPDTSDSNGDGATYAGPDTTDSF"
     gene            complement(1314..>1991)
                     /locus_tag="VGB85_30730"
     CDS             complement(1314..>1991)
                     /locus_tag="VGB85_30730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HEY0138514.1"
                     /translation="ADHPEIILQPYMFLEMFDTLDKLVDRCQARGVRLWPGNNLGYFG
                     PIERRLRASQKKHWRGCTAGVSVMGIESNGGIKNCPSLGGENNVGGSWRVHGVKKIWE
                     ESYQLGYVRNRTVDDLWGYCRECYYAETCMAGCTAAAEPLLGRPGNNPFCHHRALMMD
                     RQGLRERIEQISGAGGKGFDNGLFRVVREHKDPEQRAAHGGPVATEEPRVSRSVEPHG
                     PGRPIDG"
BASE COUNT          291 a          737 c          665 g          298 t
ORIGIN      
        1 cgaccctgcc ctcgccaccg tcgccgactg gctgcgccac gactatggcg tcgagcgctg
       61 gggcaccatc gtctcgcacc tcgccttctc gctgcacctg ctgcaccacc acctcgccgg
      121 tcgccccgcg gccttcgtgt acaccgagct gtacatgcag atcctccgct cgctgcgccc
      181 cggcggggtg ttcgcgtacg cgcccgggct gccgttcctc gagcgcctgc tgccgcgcgg
      241 cctgtatcaa tgcacgcgcc tgacggcccc gccgggcccg ggcctgcgcg cagcggcggc
      301 ggataccggc ctgcagctcg ggcacgcgac ccaggtgcgc aggctttgat cctttcttaa
      361 aaaaaaaatg cgctcgcagc gtcgcggcca ggcaccgcgg tctggggtcg atcagaccca
      421 gcacccgtcg cagtcgatcg cgctcgacgg ccatccggcc gatcgccgcc acgatgccgc
      481 gtaacagcgc tggggtcact gcgacgatcc gagctcgagc accgccgcaa tcgtagcgcg
      541 actcgctcgc gcggcggcgc tcgtccgcgc gatcggcctc gctatggctc gcaggacgtc
      601 cgtgatacgt tccgacgatg cgtgcttatc aggattgccc ggggtgtgcg tgtctggttg
      661 gggcccatga ggcggcgtgt ccgttctgcg gtcaggcgct gcgcagcgtc actgcgtcgg
      721 ggtggttgac cctcggcctg gtgctctcgc tcggcacagt gtcgatctcg tgcggcgaca
      781 aggacggcga gaccgacgac accgtcaccg catcgaacag cgacagccag ggcccggaca
      841 gcaacggcga cgtcaccgac accaatccct atcccgacgg cgtcacctac gccggcccgg
      901 acgagtcctc cgtctccgac ccctacgcct ccacgagcga cagcagcgac ccgaacacga
      961 ctccggacag ctccccggac ggctcgacct acgcgggccc cgacgagaac agcacgacca
     1021 ccggcgacga gagctccggc agcaccggcg ccgacaccga gggcagcagc ggcagcaccg
     1081 gcgacaccga gggcggcagc ggctcgacca cgacgcccga cacgagcgac agcaacggcg
     1141 acggggcgac ctacgccggc cccgacacca ccgactcttt ttgagagaag aggtgtacgc
     1201 cgggcctgtc cctccggaca ggcggcgtca cccgctcgtg cgcgctgtct cttcagaccg
     1261 gccgctcagc cgacccggct cgcggcacct gtccctccga acaggtcgac ctgtcagccg
     1321 tcgatcggtc ggcccggccc gtgcggctcg accgaccgcg acacgcgggg ctcctcggtc
     1381 gcgaccggcc cgccgtgcgc cgcgcgttgc tcgggatctt tgtgctcgcg cacgacgcgg
     1441 aacaggccgt tatcgaagcc cttgccgccg gcgccggaga tctgttcgat gcgctcgcgc
     1501 aggccctgac ggtccatcat cagggcgcgg tggtggcaaa acgggttgtt gccggggcgg
     1561 ccgagcagcg gctcggcggc ggcggtgcag ccggccatgc aggtctcggc gtagtagcac
     1621 tcgcggcagt agccccacag gtcgtcgacg gtgcggttgc ggacgtagcc gagctggtac
     1681 gactcctccc agatcttctt gacgccgtgc acgcgccagc tgccgccgac gttgttttcg
     1741 ccgccgaggc tcgggcagtt cttgatcccg ccgttgctct cgatgcccat gaccgacacg
     1801 ccggccgtgc agccgcgcca gtgcttcttc tgcgaggccc gcaggcgccg ctcgatcggg
     1861 ccgaagtagc cgaggttgtt gccgggccac aggcgcacgc cgcgggcctg gcagcggtcg
     1921 acgagcttgt cgagggtgtc gaacatctcg aggaacatgt agggctggag gatgatctcc
     1981 gggtggtcgg c
//
LOCUS       DASWNJ010001837         2118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_384409, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001837 DASWNJ010000000
VERSION     DASWNJ010001837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384409"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..1435
                     /locus_tag="VGB85_30735"
     CDS             164..1435
                     /locus_tag="VGB85_30735"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEY0138515.1"
                     /translation="MGEATPLALIDGNNFYVSCERVFDPGLAGVPVLVLSNNDGCAIA
                     RSEEVKALGVRMGEPAFRLRELIGRHRIRVFSSNHVLYGDMSRRVNEVLAGFSPNVEV
                     YSIDETFLDLSGFGQVDLWAYAQQMRSTVRQWTGIPTCVGLGPTKTLAKLANAVAKRN
                     PAFGGVCDLTHEAVRSAVLRALPVGEVWGVGAATAGRLAALGVTTVAGLRDMEPKHAR
                     LLGTVVLERIVHELRGLPCLPLELVPAGRKGLAVTRSFGEPTDVLAKVIEATAAHATR
                     AAEKLRVHGVVAGQLTAFLHTSPHREGRRHHGARSTRLDPMTSDTRELVAAAGRCVGA
                     AWREGCAYVKAGVVLDDLRPADQVPATLFDPPREKSAALMRAVDELNARYGRDTVFPA
                     AVGVERPWRQRAAHHSPRYTTRLSDLPRVRA"
     gene            1773..>2118
                     /locus_tag="VGB85_30740"
     CDS             1773..>2118
                     /locus_tag="VGB85_30740"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY0138516.1"
                     /translation="MAKKAAKLAVLNQKGGVGKTTISVNLAYGLARAGKRTLLIDLDP
                     QAHSSIIFCPEIPRDGTVKEVFERTKVDLAPLIRSAEVRGEAVANLWIVPSNIHLAVT
                     AERMISQHYRERR"
BASE COUNT          338 a          702 c          759 g          319 t
ORIGIN      
        1 gacggcgaga tgacgatcaa gcggctacgg cggctccgcg acggccgcat ggcgctcaag
       61 gccgagcatc ccgactatcc cgggctgatc atctgcgagg agaccccggc cgaggtgtgg
      121 ggggtcgttg tcggcgtcgt ccgcaagctc gcctgagcgg gccgtgggcg aggcgacccc
      181 cctcgccctg atcgacggca acaacttcta cgtctcgtgc gagcgcgtct tcgacccggg
      241 gctcgcgggc gtgccggtgc tggtgctcag caacaacgac ggctgcgcca tcgccaggag
      301 cgaggaggtg aaggcgctcg gcgtgaggat gggcgagccg gcgttcaggc tgcgcgagct
      361 gatcggccgg caccgcatcc gcgtgttctc ctcgaaccac gttctgtacg gcgacatgag
      421 ccgccgcgtg aacgaggtcc tcgcggggtt ctcgccgaac gtcgaggtct actccatcga
      481 cgagaccttc ctcgatctct ccggcttcgg ccaggtcgac ctctgggcgt acgcgcagca
      541 gatgcgatcg accgtccgac agtggacggg gataccgacc tgcgtcggcc tcggcccgac
      601 caagaccttg gccaagctcg ccaacgcggt ggccaagagg aacccggcct tcggcggagt
      661 ctgcgacctc acgcacgagg ccgtgcggtc cgcggtgctg cgcgcccttc ccgtcgggga
      721 ggtgtggggc gtcggcgcgg cgacggcggg gaggctggcc gcgctcggcg tgacgacggt
      781 cgcgggcctg cgcgacatgg agccgaagca tgcccgcctg ctcggcacgg tggtcctgga
      841 gcggatcgtc cacgagctgc gcgggctgcc ttgtctcccg ctcgagctgg tgccggccgg
      901 gcgcaagggg ctcgcggtga cgcgctcctt cggggaaccg accgacgtgc tcgccaaggt
      961 gatcgaggcg accgccgccc acgcgacgcg ggccgccgag aagttgcgtg tgcacggagt
     1021 ggtcgccggc cagctcaccg ccttcctgca caccagcccg caccgcgagg ggaggcggca
     1081 ccacggcgcg cgctcgacgc ggctcgaccc gatgacgagc gacacgcgcg agctggtcgc
     1141 ggccgccggc cgctgcgtcg gggcggcgtg gcgggagggt tgcgcctacg tgaaggccgg
     1201 ggtcgtgctc gacgacctgc gcccggccga tcaggtgccg gcgaccctgt tcgacccgcc
     1261 gcgggagaag tcggcggcgc tgatgcgggc ggtggacgag ctgaacgcga ggtacgggcg
     1321 ggacacggtg ttcccggccg cggtcggcgt cgaacggccg tggcggcagc gcgccgcgca
     1381 ccactcgccc cgctacacca cccggctttc cgacctgccg cgcgtgcggg cgtgaagagg
     1441 tgccgcaacg gcgcacatcc acttcaagca ctacttttgt agtgctccaa tgcgctacta
     1501 tcgtagttct actgagcact acatatatag cacttgaaag atgagggagg tgcgaggcgg
     1561 aaggcgagcc catgcccaac atgtcggcgc ctcgaaccca cgcatcttga tagtttgctc
     1621 ctcggttcgg ccagaacgag ctgccttatc gcggcgcgtg ccacagtcgg ctgagaacgc
     1681 cagcgctggg tagcaaatcg agcatctatg cttgacggca ctacggccgt agtgcgtgct
     1741 ggcgctacga ctgtagtggc aagggggaga cagtggctaa gaaggcggcc aagctggcgg
     1801 tgcttaatca gaagggcggg gtgggcaaaa ccaccatctc ggtcaacctg gcctacggcc
     1861 tagcgcgtgc cggcaagcgc acgctgctga tcgacctcga cccgcaagcc cactcgagca
     1921 tcatcttctg tcctgagatc ccgagggacg gcacggtcaa ggaggtgttc gagcgcacga
     1981 aggttgacct cgcgccgctc atccggtcag cagaggtgag gggggaggcg gtcgccaatc
     2041 tctggatcgt gccctccaac atccacctag cggtgaccgc cgagcggatg atctcgcagc
     2101 actatcgcga gcgccggc
//
LOCUS       DASWNJ010001838         3269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_526496, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001838 DASWNJ010000000
VERSION     DASWNJ010001838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526496"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(368..616)
                     /locus_tag="VGB85_30745"
     CDS             complement(368..616)
                     /locus_tag="VGB85_30745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138517.1"
                     /translation="MPQPITPDRVSFKRDHLEIEADEGEYAVDQSLEIALPDPYGVVS
                     ATVTAVHGRTLHAMWELDHDAMVRGYFTPIIEDPGPTS"
     gene            complement(729..>3269)
                     /locus_tag="VGB85_30750"
     CDS             complement(729..>3269)
                     /locus_tag="VGB85_30750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEY0138518.1"
                     /translation="HDRQFHVIVDPRRLVKHGLTLDEVARVLELGNRNAGGGVLEQAG
                     ESQLIQGQGLVGSIADLEAMVLANVDGKLIRLGDVADVREGHEIRRGAVTAEGQGEAV
                     LGLGFMLMGENSREVTRQLETRLEQVRHSLPEDVAVTPVYSRTNLVDAVLRTVRNNLL
                     EGALLVIAVLFVFLGSFRAGLIVAVAIPLSMLFAFNAMLQFGIAGSLMSLGAIDFGLV
                     VDSSVILVENAARRLEEDDTNRSIREIVRDAAVEVRKPTLFGELIIAIVYLPILALEG
                     VEGKLFEPMALTVIFALAGSMLLSVTLMPVLASFALKKGRGAPDNRLVRWLKGRYRRS
                     LGWALEWRKGVLVVAGLLVVLAAILAARLGSEFVPRLGEQAVVINTVRVAGISLDESV
                     RYGTKIERRLRDRFPDEVEHIWTRTGTAEVATDPMGLEVSDVFITLTPRQQWTRGETQ
                     DDLVRAMAAELDGMPGMRSVFTQPIEMRVNEMVAGVRADVGIKLFGDDFEVLREKADE
                     IRRAVETIPGASDVTVEQLTGQAMLVIEVDRQAAGRHGIPVGEILEVIESLGTRKVGE
                     VVEGQRRFDLVLRLSDAARGSAEEIGRVLITTASGAQIPLARVARIDVIEGPSTIQRE
                     WAKRRVTVQANVRGRDLGSFVAEVRRTLRDDVSLPDGYYTTLGGQFENLERAQRRLLV
                     VVPIALLLVFSLLYLTYGRAVDAVRVFTGVPFGAVGGIVALWMRDMPFSISAGVGFIA
                     LSGIAVLGDMVLVSRVMQLLERGQGPSEAIREAAETRLRPVLMTATVAAFGFVPMALA
                     TGVGAEVQRPLATVVIGGVVTSTIATLFVLPVLYAAFGARRPEHEGSA"
BASE COUNT          555 a         1170 c         1077 g          467 t
ORIGIN      
        1 ggcgcccagg gccgcagcgc ccgcgatcat gtgcaacatc ttgcgacggg ggatcatgcc
       61 actgcatgac gtaacctcgg ccgggcccca ggccgggccg cgaagccggg ccattgcgcc
      121 gccaggcccg cggatcgtcg cccgcgaggt gtggacattc gcggccgccg cagatcgcca
      181 ggcgcggcgg gggtccgcgg gccaccatgg cgatcgccag gtgaccgggg atattcctcg
      241 aatcatggga cgcgaccccg gctcggtgcg cgagctgtta ctcagggagc gccgcgtctc
      301 gggcaccgct gcgagcgccg agtcgcgcgc aggcccgatg ccgcgctccg gcgaactcgg
      361 atccggctca ggaggtaggg cccgggtcct cgatgatcgg ggtgaagtag ccccggacca
      421 tcgcatcgtg gtcgagctcc cacatcgcgt gcaaggtgcg gccgtggacg gctgtcaccg
      481 tcgcggagac gacaccataa gggtccggta gcgcgatctc gagggactgg tcgacggcgt
      541 actcgccctc atcggcctcg atctcgaggt ggtcacgctt gaacgacacg cgatcgggcg
      601 taatgggttg cggcatgggc ctcggcgcga gcttatgccc gcgccgcgga gctgtctctc
      661 tggggacaac cccggtcttc agggaccgag tgtctggccg cagcgccgcc tgcacgaggc
      721 cgtccgcttc acgccgagcc ctcgtgctcc ggccgacgtg cgccgaacgc cgcgtagagc
      781 acgggcaaca cgaacagcgt ggcgatcgtc gacgtgacca cgccgccgat gaccacggtc
      841 gcgagcgggc gctgaacctc cgcgcccacg ccggtggcga gcgccatggg cacgaacccg
      901 aacgcggcca cggtcgcggt catcagcacc ggccgcaggc gagtctccgc agcctcgcgg
      961 atcgcctcgc tcggcccctg accgcgctcg agtaattgca tgacccgcga cacgagcacc
     1021 atgtcgccga gcacggcgat acccgaaagc gcgatgaacc cgaccccggc cgagatcgag
     1081 aacggcatgt cgcgcatcca caaggccacg atgccaccga ccgcgccgaa gggcacaccg
     1141 gtgaagacgc gaaccgcgtc gaccgcacga ccgtaggtca ggtacagcag cgagaacacc
     1201 aacaacaggg cgatcgggac gaccaccaac aggcgacgct gggctcgctc gaggttctcg
     1261 aactgcccgc cgagggtggt gtagtagccg tcgggtagac tgacgtcatc gcgcagggtt
     1321 cggcgaacct ccgccacgaa cgaacccagg tcgcgcccac gcacgttcgc ctgcacggtc
     1381 acgcgacgct tggcccactc gcgctggatc gtcgagggcc cttcgatcac gtcgatgcgc
     1441 gcgaccctcg ccaacgggat ctgggcgccc gacgccgtgg tgatcagcac acgaccgatc
     1501 tcttcggcag agccgcgggc cgcatcactc aggcgaagca cgagatcgaa gcgccgctgg
     1561 ccctcgacga cctcgcccac cttgcgggtc cccagcgatt cgatgacctc gaggatctcc
     1621 ccgaccggaa ttccgtggcg gccggcggcc tggcggtcga cctcgatcac gagcatcgcc
     1681 tggcccgtca gctgctcgac ggtcacgtcg ctggcgcccg ggatggtttc gacggcccgg
     1741 cggatctcgt ccgccttttc gcgcaggacc tcgaagtcgt cgccgaacag cttgatgccg
     1801 acatcggccc ggacccccgc gaccatctcg ttcacgcgca tctcgatcgg ctgcgtaaag
     1861 acgctacgca tgcccggcat gccgtcgagc tcggcggcca tggcccggac gagatcgtcc
     1921 tgggtctcgc ctcgcgtcca ttgctggcgt ggcgtgagcg tgatgaacac gtccgagacc
     1981 tcgagaccca tcggatcggt ggcgacctcg gcggtgccgg tccgcgtcca gatgtgctcg
     2041 acctcatccg gaaagcgatc tcgcagccgc cgttctatct tcgtcccgta gcgaacggac
     2101 tcgtcgagcg agataccagc gacgcgcacg gtgttgatga cgaccgcctg ttcgccgagc
     2161 cggggcacga actccgagcc gagccgcgcg gccaggatcg cggccaggac cacgagcaat
     2221 ccagcgacca ccagcacgcc cttgcgccac tcgagcgccc aaccgagcga gcgtcggtag
     2281 cggcccttga gccagcgcac cagccggttg tcaggggctc cgcggccctt tttcaaggcg
     2341 aagctggcga gcacgggcat cagcgtgacc gagaggagca tcgagccggc cagggcgaag
     2401 atcaccgtga gcgccatcgg ctcgaacagc ttgccctcca cgccctcgag cgcgaggatc
     2461 ggcagataga cgatcgctat gatcagctcg ccgaacagcg tgggcttgcg gacctcgacc
     2521 gcggcgtcgc ggacaatctc ccggatactc cggttggtgt cgtcctcttc cagccgccgg
     2581 gccgcgttct cgaccaggat gaccgaactg tcgacgacca ggccgaagtc gatcgcgccc
     2641 aggctcatca ggctgccggc gatcccgaat tgcagcatcg cgttgaacgc gaacagcatc
     2701 gacaatggaa tcgcgaccgc gacgatcagc cccgcccgaa aactcccgag gaacacgaac
     2761 aacaccgcga tgacgagcag cgcgccctcg aggaggttgt tgcggaccgt tcgcagcacc
     2821 gcgtcgacca ggttggttcg gctgtagacc ggcgtcaccg cgacgtcctc gggcaggctg
     2881 tggcgcacct gttcgagccg cgtctcgagc tgccgggtga cctcgcggct gttctcgccc
     2941 atcagcatga agccgagccc gagcacggcc tcgccctggc cctccgccgt caccgccccg
     3001 cgacggatct cgtggccctc acggacatcc gcgacgtcgc cgagccggat cagcttgccg
     3061 tcgacgttgg cgagcaccat cgcctcgaga tccgcgatgc tgccgacgag gccctgcccc
     3121 tggatcagct gcgattcgcc ggcttgctcg agcacaccac caccggcgtt gcgattgccc
     3181 agctcgagga cgcgggcgac ctcgtcgagg gtcaagccat gcttgaccag ccgacggggg
     3241 tcgacgatga cgtggaactg ccggtcgtg
//
LOCUS       DASWNJ010001839         1787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_211116, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001839 DASWNJ010000000
VERSION     DASWNJ010001839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211116"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..359
                     /locus_tag="VGB85_30755"
     CDS             150..359
                     /locus_tag="VGB85_30755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138519.1"
                     /translation="MATFTGTVRYNDLEGGFYELVTDKGDTYRLSKCTAKAGQQVKVK
                     GKLEGGGFGIHMSGPSIEVESISEK"
     gene            complement(446..527)
                     /locus_tag="VGB85_30760"
     tRNA            complement(446..527)
                     /locus_tag="VGB85_30760"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(491..493),aa:Leu,seq:cag)
     gene            complement(624..>1787)
                     /locus_tag="VGB85_30765"
     CDS             complement(624..>1787)
                     /locus_tag="VGB85_30765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138520.1"
                     /translation="AKEAVEQDYHRLRGEVDAKRRSVEQLQQLVALGAGTGRGTASDH
                     ELQALRALLAQQTSEHAERLAQQEQQQRLLNEHQREKLARVQLEATIRAEEQEYLLYQ
                     LDTAEQRIWEMTDATDRSAARLAAGLAQLDKQKEQYEDLIDQLEVSRTLLAEAQDRIV
                     ELERQLASDKAKLARLTLDTRVPFEREDEESADGSAFDVMVVDHGEDDEDMEIAPPLQ
                     LDPDRPDPLAGIDFDDDEESMAVRLASPFQLTGAFAAAKARVVPDRDDDGPSMVLPSD
                     RLHIELDDDDDDDPLFKPAPVQPARAPVREPPREQPRRPQQQQPLRTLPFLDDDDMFS
                     IDTDSIELATPTPPPPPPDRPGFDQKLTDDPNSRIVIEILDDEAWPDDVEDKD"
BASE COUNT          253 a          615 c          631 g          288 t
ORIGIN      
        1 aggcccgcgc ggtcgcctgg tcgcgccaca ggcccgcgcc gccgcccggg tcgacccccg
       61 ccagcagcca caaggtcgca ggcccggcca tcgcgccgaa gcctaaccgc cggtatgcaa
      121 actcgccagg ccccggctac actggccgca tggcgacctt caccggcact gtacgctaca
      181 acgacctcga gggcggcttt tacgagctcg tgaccgacaa gggcgacacc taccgcctca
      241 gcaagtgcac cgcgaaggcc ggtcagcagg tcaaggtcaa gggcaagctc gagggcggcg
      301 gcttcggcat ccacatgagc ggcccgagca tcgaggtcga aagcatcagc gagaagtagg
      361 cgccggaacc ggcgactcga agatcaacgc ccccagaaac aacaaggccc tggaccgtga
      421 ggaccagggc cttggtgttt tcaaatgcga ggaaagggac tcgaaccccc acgtcttgcg
      481 acacacggac ctgaaccgtg cgcgtctacc aatttcgcca ccctcgcggt tgggagaggg
      541 aagctaatcg accgccgcgg cgccgtcaag accgaagttg cggagggcgt cccggggccg
      601 tttcgcgccg agcccgcacc tgatcagtcc ttgtcctcga cgtcgtccgg ccaggcctcg
      661 tcgtcgagga tctcgatgac gatgcgggag ttcgggtcgt cggtgagctt ctggtcgaag
      721 ccggggcggt ccggaggcgg cggcggtggt gtcggcgtcg ccagctcgat cgagtcggtg
      781 tcgatcgaga acatgtcgtc gtcgtccagg aaaggcagcg tgcgcagcgg ctgctgctgc
      841 tggggacgcc ggggctgctc gcgcggcggc tctcgtacgg gcgctcgcgc cggctgaacc
      901 ggcgccggct tgaacagcgg gtcgtcgtcg tcgtcgtcgt cgagctcgat gtgcaggcgg
      961 tcggagggca gcaccatcga gggcccgtcg tcgtcgcggt ccgggacgac ccgcgccttg
     1021 gccgcggcga aggcgccggt cagctggaag gggctggcga ggcgcacggc catgctctcc
     1081 tcgtcgtcgt cgaagtcgat gccggcgagc gggtcgggtc ggtcggggtc gagctggagc
     1141 ggcggcgcga tctccatgtc ctcgtcgtcc tcgccgtggt cgacgaccat cacatcgaag
     1201 gcgctgccgt cggccgactc ctcgtcctca cgctcgaagg gcacgcgggt gtcgagggtc
     1261 aggcgcgcca gcttggcctt gtcgctcgcc agctgccgct cgagctcgac gatccggtcc
     1321 tgcgcctcgg ccagcaaggt gcgtgacacc tcgagctggt cgatcaggtc ctcgtactgc
     1381 tccttttgtt tgtcgagctg ggcgaggccg gcagcgagcc gcgcggcgct gcgatcggtc
     1441 gcgtcggtca tctcccagat ccgctgctcg gcggtgtcga gctggtagag caggtactcc
     1501 tgctcctcgg cccggatcgt cgcctcgagc tgcacccgcg ccagcttctc gcgctggtgc
     1561 tcgttgagca gccgctgctg ctgctcctgc tgggccaggc gctcggcgtg ctcgctggtc
     1621 tgctgggcca gcagcgcccg cagggcctgc aactcgtggt cgctcgcggt gccgcggccg
     1681 gtccccgcgc cgagcgcgac gagctgctgg agctgctcga ccgagcgccg cttggcgtcg
     1741 acctccccgc gcaggcgatg gtagtcctgc tcgaccgcct cctttgc
//
LOCUS       DASWNJ010001840         2239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_343974, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001840 DASWNJ010000000
VERSION     DASWNJ010001840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343974"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VGB85_30770"
     CDS             <1..489
                     /locus_tag="VGB85_30770"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0138521.1"
                     /translation="AVVAIGRRLQVLQSLEIQVKRLDAHPHQSDAPTHEAEVFVDGVN
                     FGYLSDGTQRLIELVIKLVDPVVTVLLLEEPETSVHPGLLRRLLAELEALGPGKQVVI
                     STHSPMVIDWTAAGDVRLVERTPEGITSVRTLSADEQARLRIYLDDDLALSDFMFSGA
                     MD"
     gene            504..1064
                     /locus_tag="VGB85_30775"
     CDS             504..1064
                     /locus_tag="VGB85_30775"
                     /inference="COORDINATES: protein motif:HMM:NF025468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HEY0138522.1"
                     /translation="MGEDETDVLTLRTILRRMIPREVGIERRNPPRGRAGCAALRKAA
                     KTYMKELHAAGCHAVILLHDLDLSGETNELNDEGQLRTRLAAIPVPPGLARLICIPVE
                     EIEAWFWADQNVLDRIGPGGKASASPHTIKRPKEQLRDLSWRAHRKYNYATTQNPELA
                     ALLDLELCARRCRSFAELQAFVAAQL"
     gene            complement(1077..>2239)
                     /gene="kbl"
                     /locus_tag="VGB85_30780"
     CDS             complement(1077..>2239)
                     /gene="kbl"
                     /locus_tag="VGB85_30780"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEY0138523.1"
                     /translation="AAILQQIREAGLYKHERVIDSAQSADIEVDGRHVLNFCANNYLG
                     LSSHPAVIAAAHAALDDRGFGMSSVRFICGTQDRHKALERRLADFVGLEDAILYSSCF
                     DANGGLFETLLGPEDAIISDALNHASIIDGIRLCKAQRHRYANDDLNQLEDLLKETQS
                     ARQRLIATDGVFSMDGNIARVKDICDLADKYGAMVHVDDSHATGFVGPTGRGSHEHNG
                     CLGRVDIITSTLGKALGGAAGGFTAARPEIVDLLRNRSRPYLFSNSVPPPIVAASLAV
                     MDLLAETGELLARLNANTRRFREGMTAAGFAIKPGVHPIVPVMLGDARVASEFAAKMS
                     NEGIYVVGFSYPVVPQGQARIRVQLSAGHTPEHVEHAITAFTKVGRELGVIA"
BASE COUNT          351 a          697 c          839 g          352 t
ORIGIN      
        1 gcggtcgtcg cgatcgggcg ccgtctacag gtgctgcagt cgctcgagat tcaggtcaag
       61 cggctcgatg cgcatcctca tcagtccgac gcgccgacgc acgaggccga ggtgttcgtc
      121 gatggcgtca acttcggtta cctgtcggac ggcacgcagc ggctcatcga gctcgtgatc
      181 aagctggtcg atccggtggt caccgtcctg ctgctcgagg agccggagac ttccgtgcac
      241 ccggggctgc tacgcaggct cctggccgag ctcgaggcgc tcgggccggg gaaacaggtc
      301 gtgatctcga cgcactcgcc gatggtgatc gactggacgg cggcagggga cgtacgcctc
      361 gtcgagcgga ccccggaggg catcaccagc gtgcgcacgc tcagcgccga cgagcaggcg
      421 cgcctgcgga tctacctcga cgacgatctg gcgctctcgg acttcatgtt ctcgggagcg
      481 atggattgag cgcgatcggg atcatgggtg aagacgagac ggacgtcctg acgctcagga
      541 cgatcctgcg tcggatgatc ccgcgggaag tcgggatcga gcggcgcaac cctccccgcg
      601 ggcgggccgg ctgcgccgcg ctgcgcaagg cggcgaagac gtacatgaag gagctgcatg
      661 cggcgggctg tcacgcggtg atcctgctgc acgacctcga cctgagcggc gagaccaacg
      721 agctcaacga cgagggccag ctgcggacac ggctcgcggc gatcccggta cctcccgggc
      781 tcgcgcgcct gatctgcatc ccggtcgagg agatcgaggc gtggttctgg gccgatcaga
      841 acgtgctcga caggatcggc cccggaggca aggcgtcggc ctcgccgcac acgatcaagc
      901 ggcccaaaga gcagctgcgt gacctgtcgt ggcgtgcgca tcgcaaatac aactacgcga
      961 ccacgcagaa ccccgagctc gcggcgctcc tcgacctcga gctgtgcgcc cggcgatgcc
     1021 gctcgttcgc cgagctgcag gcgttcgtcg cggcgcagct ttgaaagaag taaggctcag
     1081 gcgatcacgc cgagctcgcg gccgaccttg gtaaaggccg tgatcgcgtg ctcgacgtgc
     1141 tcgggcgtat ggccggcgga cagctggacg cggatgcggg cctggccctg cgggacgacg
     1201 gggtagctga agccgacgac gtagatgccc tcgttcgaca tcttcgccgc gaactcgctg
     1261 gcgacgcgcg cgtcgcccag catcaccggg acgatcgggt gcacgccggg cttgatcgcg
     1321 aagcccgcgg cggtcatccc ctcgcggaag cgcctggtgt tggcgttgag gcgggcgagc
     1381 agctcgccgg tctcggccag caggtccatg accgccagcg aggcggcgac gatcggcggc
     1441 ggcacggagt tgctgaagag gtaggggcgc gagcggttgc gcaggaggtc gacgatctcc
     1501 gggcgcgcgg cggtgaagcc acccgcggcg ccgccgagcg ccttgccgag cgtcgaggtg
     1561 atgatgtcga cgcggccgag gcagccgttg tgctcgtgcg agccgcggcc ggtcggaccg
     1621 acgaagccgg tcgcgtggct gtcgtcgacg tggaccatcg cgccgtactt gtcggcgagg
     1681 tcgcagatgt ccttgacgcg ggcgatgttg ccgtccatcg aaaacacgcc gtcggtggcg
     1741 atcaggcgct ggcgagcgga ctgggtttct ttaaggaggt cctcgagctg gttgaggtcg
     1801 tcgttggcgt agcggtggcg ctgggccttg cacaggcgga tgccgtcgat gatcgaggcg
     1861 tggttgagcg cgtcggagat gatcgcgtcc tcggggccga gcagggtctc gaacaacccg
     1921 ccgttggcgt cgaagcagga gctgtagagg atcgcgtcct cgagaccgac gaagtcggcg
     1981 agccggcgct cgagcgcctt gtggcggtcc tgggtgccgc agatgaagcg cacgctcgac
     2041 atgccgaagc cgcggtcgtc gagcgcggcg tgggcggcgg cgatcactgc cgggtgcgac
     2101 gacaggccga ggtaattgtt ggcgcagaag ttgaggacgt ggcgcccgtc gacctcgatg
     2161 tcggcggact gcgccgagtc gatcacgcgc tcgtgcttgt agagcccggc ttcgcggatc
     2221 tgctggagga tggcggcgt
//
LOCUS       DASWNJ010001841         7184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_384460, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001841 DASWNJ010000000
VERSION     DASWNJ010001841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7184
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384460"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /locus_tag="VGB85_30785"
     CDS             complement(<1..1170)
                     /locus_tag="VGB85_30785"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEY0138524.1"
                     /translation="MRYRPPSILSHLTALQAEHGWLAQDSLVELAQHIGVPLHRIEGV
                     ASFYPHFRRSPPPRHRVEVCRDLSCAMAGGGQPLRAAAEAHAASTGERVEVVEVSCLG
                     RCDRAPAALVHHTPVQGPAQLGACLGGQPVPEDMSPRTYRIDPYGTAGERYATARAVA
                     AGGPATVERVIAELKAAGLRGMGGAGFPTAIKWSTVRATPGAEKFVICNADESEPGTF
                     KDREIKAAVPHLVWEGMIIAALVVGAQRAIVFIRHEYEQERVRMEAERQVAARVAADF
                     GVELEIFVSPGGYIMGEETALLEALEDRRGEPRNKPPFPGVAGLFDRPTLINNVETLA
                     MVTAILARGLRVNADAGEMPGAQWWQAQGVRGCHGLKFISISGDVATPGVYEIETG"
     gene            complement(1183..2349)
                     /locus_tag="VGB85_30790"
     CDS             complement(1183..2349)
                     /locus_tag="VGB85_30790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138525.1"
                     /translation="MHRAPGLVLPLVLAACTNSGTPPDGATDKAAAAPAQPTAAAGPT
                     LPGRLVFLAEDAEEERTGAIVAMRPDGSERKELIRGAGLYPAAVDPSGAALALIAVDE
                     QGGGHAEVLKIVPWTSSGLGKPIWESPPSSHVRNPSWGPDGRFIVFEAALDSFRDLYR
                     LDLPAREPGHGDVPAPAKLLRLTDNAEGNFEPAVSADGQHIAFVSSRDGNAEVYRMKS
                     DGSEQRRLTNYPLDDWGPLWAPDGKTIAFLSNREQIDRIYLMAPDGSAQRRFTADQAP
                     VRDPDAPLGDEPHETDPVFGPDGLHLAYCVRVGSQGASLRIAPIAGGAPVVLSDGRFA
                     DRSPVWSPDGAALVFVSNREGPELDLFRVARDGSGVARLTDRPGADWLPRWSSR"
     gene            complement(2441..4141)
                     /locus_tag="VGB85_30795"
     CDS             complement(2441..4141)
                     /locus_tag="VGB85_30795"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY0138526.1"
                     /translation="MQRTTYLSLSSGLLTALALLAPGCDAPDDTDLNVAAVEDDAVNE
                     NINGIVAQEDAEPLDPPTHDAGLALSGIDADFTDAAAEFDVPAQLLQAIGYTETQWQM
                     VEGLSEFDGQEPAFGVMALRGERLALGAKLAGEDIEAVKTDRRANIRAGAALLSVDAD
                     ELGFDRSNLAAWAPAVARFSGIPDSLPEIQANYVHNDVYAAMRDGATVKDFAGKPIAR
                     LEPTSGAVADFALAPNPTAAPGPDYSGSIWRPSPNYSSRPTGVKMVVIHSCEGAYSGC
                     WGWLVNKASGVSAHYVVKEDGNEISQLVKEASKAWHVAATYSCSNNSSKECGLNGQST
                     NNFSVGIEHAGFAKTTQWSANLIDKSAKLVCDITKGHNIPRDKYHIVAHGQLQPYNRI
                     DPGPNWPWATYLSKINTHCGAAPPPPPPPPPPAGSTIVIDSNNSNNIATQAKIAVSAN
                     WTSTAGTPGYYGSGYFFANTEAVSDGAEFSFYLPAAATKTVDAWWTAGTNRSTTAPFV
                     AFNAAGTKLTTFTANQSINGGKWVQLGTANFSAGWNKIVLSRWTTAGKVVIADAVRVR
                     "
     gene            4679..6292
                     /locus_tag="VGB85_30800"
     CDS             4679..6292
                     /locus_tag="VGB85_30800"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HEY0138527.1"
                     /translation="MHRSPWVRSLRVIVLGSTFIVAGGLAGPGPAAALPGFYVGSADA
                     PPRSDATMVVLMRDGRRTVVSMQPGYIGPAKDFVIVVPVPAIIKEQDVKTLPRELFDR
                     IDGLTAPRLVETWEQDPCVLDEPAAPPAAADASAVVEPDPPAPAVEVAARFTAGEYQI
                     VTLAAKQPSALADWLRDNGLVLPSGADEALRPYIEQGMKFFVARVDASALELDERGRA
                     TLSPLRFHYDSDTLELPLRPGLLSSAGAQDTVIHILARERHVAANVANATIPTNLEVK
                     DTAHGSFAPFYVALFDATREHNPGAVITEYAWPANSCEPCPQRPLDLADLMTLGVDVL
                     PGLGVRPEKRGAQWTVPDAFVLTRLHVRHDRGEKIEDLVFKPAGPIVGGRELAVAGQL
                     ERGAQPTSELNAFQARYVVRHRWTGRVDCKEPHPGRWGGPPADWKGEVREAIVARDLA
                     AVPRDASLAGYVAHDIPEIRYVVRADEPPARKPAIPAANPAPAPDVEVVLPGEPVAST
                     RTSCGCTGGEAPAGWLGLLALLAIRRRRP"
     gene            6334..>7184
                     /gene="dusB"
                     /locus_tag="VGB85_30805"
     CDS             6334..>7184
                     /gene="dusB"
                     /locus_tag="VGB85_30805"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEY0138528.1"
                     /translation="MNEANAQGPARPGEFAALQVGPLRVWPPVVLAPMAGVTNYPFRA
                     LCRRFGAGLYVSEMITARPLVRGDAQSRRLADFGPGEAPRSLQLYGVDPADIGAAVRW
                     LVGEGRVDHVDLNFGCPVRKVTRKGGGAAIPLKRNLLRAIVRAAVQAAGAVPVTMKFR
                     IGVDDALTTFETAGKIGEDEGCAAVGLHARTAAQGYSGTARWAAIARLKQHVTRIPVL
                     GNGDIWEASDALRMMRETGCDGVIVGRGCLGRPWLFRDLNDVFHGREPRNPPTLGEVV
                     DIMITHAR"
BASE COUNT          963 a         2467 c         2698 g         1056 t
ORIGIN      
        1 gccggtctcg atctcgtaca cgcccggcgt ggcgacgtcg ccgctgatcg agatgaactt
       61 gagcccgtga cagccgcgca cgccctgcgc ctgccaccac tgcgcgccgg gcatctcgcc
      121 cgcatccgcg ttgacccgca ggccacgcgc gaggatcgcc gtgaccatcg ccagcgtctc
      181 gacgttgttg atcagcgtcg ggcgatcgaa caggccggcg acgccgggga acggcggctt
      241 gttgcgcggc tcgccgcggc ggtcctcgag cgcctcgagc agggccgtct cctcgcccat
      301 gatgtagccg ccgggcgaca cgaagatctc gagctcgacg ccgaagtccg cggcgacccg
      361 cgcggcgacc tggcgctcgg cctccatgcg cacgcgctcc tgctcgtact cgtggcggat
      421 gaagacgatc gcccgctgcg cgccgacgac cagcgccgcg atgatcatgc cctcccatac
      481 caggtgcggc accgcggcct tgatctcgcg gtctttgaaa gtgccgggct cgctctcgtc
      541 ggcgttgcag atcacgaact tctcggcgcc cggcgtcgcg cgcacggtgc tccacttgat
      601 cgccgtcggg aagccggccc cgcccatgcc gcgcaggccg gcggccttga gctcggcgat
      661 cacccgctcg acggtcgccg ggccgccggc cgcgaccgcc cgcgccgtcg cgtagcgctc
      721 gcccgccgtc ccgtacgggt cgatgcgata ggtccgcggg gacatgtcct cggggacagg
      781 ctggccaccg aggcacgcgc cgagctgcgc ggggccctgc acgggggtgt gatgcacgag
      841 cgccgcgggg gcgcggtcgc agcggccgag gcacgacacc tcgacgacct cgacgcgctc
      901 gcccgtgctc gccgcgtggg cctcggccgc ggcgcgcagt ggttgaccgc cgcccgccat
      961 cgcgcagctg aggtcgcggc agacctcgac gcggtggcgc ggcggcgggc tgcggcgaaa
     1021 gtgcgggtag aacgaggcga cgccctcgat gcggtgtagc ggcacaccga tgtgctgggc
     1081 gagctcgacg aggctgtcct gcgcgagcca gccgtgctcc gcttgcaggg cggtcaggtg
     1141 cgagaggatg ctcggcggcc ggtagcgcac gcgcgcagga tatcagcgcg acgaccagcg
     1201 tggcagccag tcggcgccgg ggcggtcggt gaggcgggcc acgccgctgc cgtcgcgggc
     1261 gacgcggaac aggtcgagct cggggccctc gcggttggac acgaacacca gggccgcgcc
     1321 gtcgggcgac cacaccgggc tacggtcggc gaagcggccg tcgctgagca ccaccggcgc
     1381 gccaccggct atcggggcga tccgcaggga ggcgccctgg ctgccgacgc gcacgcagta
     1441 ggcgaggtgc aggccgtccg ggccgaacac cgggtcggtc tcgtggggct cgtcgccgag
     1501 cggcgcgtca ggatcgcgga cgggggcctg gtcggcggtg aagcggcgct gcgcgctgcc
     1561 gtcgggcgcc atcagataaa tgcgatcgat ctgctcgcgg ttgctgagga acgcgatcgt
     1621 cttgccgtcc ggcgcccaca gcgggcccca gtcgtcgagc ggatagttgg tcaggcgccg
     1681 ttgttcactg ccgtcggact tcatgcgata gacctcggcg ttgccgtcgc ggctcgaaac
     1741 gaaggcgatg tgctggccgt cggccgacac ggccggttcg aagttgccct cggcgttgtc
     1801 ggtcaggcgc agcagcttgg ccggcgccgg cacgtcgccg tggcccggct cgcgcgccgg
     1861 gaggtcgagg cggtagaggt cgcgaaagct gtcgagcgcc gcctcgaaca cgatgaagcg
     1921 gccgtccggg ccccagctcg ggttgcgcac gtggctcgac ggcgggctct cccagatcgg
     1981 cttgccgagc ccgctcgagg tccacggcac gatcttcagg acctctgcgt ggccgccgcc
     2041 ttgctcgtcg acggcgatca gggccagcgc ggccccgctg ggatcgaccg cggccgggta
     2101 cagcccggcg ccgcggatca gctccttgcg ctccgagccg tcgggtcgca tcgccacgat
     2161 cgccccggtg cgctcctcct cggcgtcctc ggcgaggaac accaggcgcc cgggcagcgt
     2221 cggcccggcc gccgcggtcg gctgcgcggg cgcggccgcg gccttgtccg tggccccgtc
     2281 cggcggggtc ccggagttcg tgcaggcggc gaggacgagg ggcaggacga gaccgggtgc
     2341 gcgatgcatg gggacgggcc cttaaatgcg ggatcgcccg ccggcacgca aggccgacgg
     2401 acgatcgagg gtacatgtcc cgagggacac gaggttgaga tcagcggacg cggacggcgt
     2461 cggcgatgac gaccttgccg gcggtggtcc agcgcgagag cacgatcttg ttccagccgg
     2521 ccgagaagtt ggcggtgccg agctggaccc acttgccacc gttgatcgac tggttggcgg
     2581 tgaaggtcgt cagcttggtg cccgccgcgt tgaaggcgac gaagggcgcg gtggtcgagc
     2641 ggttggtgcc cgcggtccac caggcgtcga ccgtcttggt cgcggcggcc ggcagataga
     2701 acgagaactc ggcgccgtcc gagacggcct cggtgttggc gaagaagtag ccgctgccgt
     2761 agtagcccgg ggtgcccgcg gtcgaggtcc agttggccga gacggcgatc ttggcctgcg
     2821 tggcgatgtt gttgctgttg ttgctgtcga tgacgatcgt cgagccggcc gggggcggcg
     2881 ggggcggcgg gggcgggggc gcggcgccgc agtgggtgtt gatcttgctg aggtaggtcg
     2941 cccacggcca gttcggaccc gggtcgatgc ggttgtacgg ctgcagctgg ccgtgcgcga
     3001 cgatgtggta cttgtcgcgc gggatgttgt ggcccttggt gatgtcgcag acgagcttgg
     3061 ccgacttgtc gatcaggttg gcgctccact gggtcgtctt ggcgaagccc gcgtgctcga
     3121 tgccgaccga gaagttgttg gtgctctggc cgttgagccc gcactccttg ctgctgttgt
     3181 tgctgcacga gtaggtcgcc gcgacgtgcc aggccttgct ggcctccttc acgagctgcg
     3241 agatctcgtt gccgtcctcc ttgacgacgt agtgggcgct gacgccggag gccttgttga
     3301 ccagccagcc ccagcagccc gagtacgcgc cctcacagct gtggatgacg accatcttca
     3361 cgccggtcgg acggctgctg tagttcggcg agggccgcca gatcgagccg gagtagtccg
     3421 ggccgggcgc ggcggtcggg ttcggcgcga gggcgaagtc ggcgacggcg ccgctggtcg
     3481 gctcgaggcg ggcgatcggc ttgccggcga agtccttgac ggtcgcgccg tcgcgcatcg
     3541 ccgcgtagac gtcgttatgc acgtagttgg cctggatctc gggcagcgag tccgggatgc
     3601 cgctgaagcg ggcgaccgcg ggggcccagg cggccaggtt cgagcggtcg aagccgagct
     3661 cgtcggcgtc gaccgagagc agcgcagcgc cggcgcggat gttggcgcgg cggtcggtct
     3721 tgacggcctc gatgtcctca ccggccagct tcgcgccgag cgcgaggcgc tcgccacgaa
     3781 gcgccatgac gccgaacgcg ggctcctgac cgtcgaactc gctcaggccc tcgaccatct
     3841 gccactgcgt ctcggtgtag ccgatcgcct gcagcagctg ggcgggcacg tcgaattcgg
     3901 cggcggcgtc ggtgaagtcc gcgtcgatgc cgctgagggc gaggccggcg tcgtgggtcg
     3961 gggggtcgag cggctcggcg tcctcctggg cgacgatgcc gttgatgttc tcgttgacgg
     4021 cgtcgtcctc gacggcggcc acattgaggt cggtgtcgtc gggcgcgtcg cagccggggg
     4081 cgagcagcgc gagcgcggtc aggagaccgg acgagaggga caggtaggtc gtacgctgca
     4141 tgatcttcaa cctcattttg agcgcggcca gagacatccg gagctgcacg ccgcgagcgc
     4201 agtcctccga tcacacatga gatgccatct ctcatacggc gaacaccggc gacgacgcaa
     4261 gtggcgagtt gcgcgcgacg aaccatcgat ctcgccgaga tttggcgagt acgacccgct
     4321 gcggtcgcat ccgggccgac ttgtagcccc cggcggcggt gacctcacga tgatcggcgt
     4381 gatcgaaacg ccggaggtcg ttactccgat catcacgagg attgcgatcg ctcgaccgca
     4441 gatatgtggg tggtcctcat caggtcattt gcatgtaccg gccgcggccg tgagacccca
     4501 ggagcgaagc cgacgcgtgc ttcacgagtg tcacatccgg cgtatccgtg agcgggtcgg
     4561 cgcccttatg tgccagcgca gaaccgcgag tggaacgcgt cgcagggcgc gatgacgccc
     4621 gaaacctcgc cgatcgccgc gccctgcgag gggccgggct cgggtaccgt cggtctcgat
     4681 gcatcggagt ccatgggtcc gctcgctgcg agtcatcgtg ctcggcagca cattcatcgt
     4741 cgcgggcggc ctggcagggc ccgggccggc cgcggcgctg cccggcttct acgtcggcag
     4801 cgctgacgcg ccgccgcgaa gcgacgcgac gatggtcgtg ttgatgcgtg acggccggcg
     4861 caccgtggtg tcgatgcagc cgggctacat cgggcccgca aaggacttcg tgatcgtcgt
     4921 gccggtgccg gcgatcatca aggagcagga cgtcaagacc ctgccgcgcg agctgttcga
     4981 tcgcatcgac gggctgacgg ccccgagatt ggtcgagacc tgggagcagg acccctgtgt
     5041 gctcgacgag cccgcggcgc cgccggccgc ggcggacgcg agcgccgtcg tcgagccgga
     5101 cccgccggcg ccggcggtcg aggtcgcggc gcgcttcacg gccggcgagt atcagatcgt
     5161 cacgctcgcg gcgaagcagc cgtccgcgct cgcggactgg ctgcgggaca acggcctcgt
     5221 cctgccgtcc ggcgccgacg aggcgctgcg gccgtacatc gagcagggga tgaagttctt
     5281 cgtcgcccgc gtcgacgcca gcgcgctcga gctcgacgag cgcgggcggg cgacgctgtc
     5341 accgctgcgc tttcactacg acagcgacac gctcgagctg ccgctgcggc cgggtctgct
     5401 gagctcggcc ggcgcgcagg acacggtgat ccacatcctc gcgcgcgagc gccacgtcgc
     5461 ggccaacgtc gccaacgcga cgatcccgac caacctcgag gtcaaggaca ccgcgcacgg
     5521 cagcttcgcg ccgttttacg tcgcgctgtt cgacgcgacc cgcgagcaca accccggcgc
     5581 cgtgatcacc gagtacgcgt ggccggccaa cagctgcgag ccctgcccgc agcgcccgct
     5641 cgacctcgcc gacctgatga ccctcggggt cgacgtgctt ccggggctcg gcgtgcgccc
     5701 ggagaaacgc ggcgcgcagt ggacggtgcc cgacgcgttc gtgttgaccc gcctgcacgt
     5761 gcgccacgac cggggtgaga agatcgaaga cctcgtgttc aagccggcgg ggccgatcgt
     5821 cggcgggcgc gagctcgcgg tcgcggggca gctcgagcgt ggcgcccagc cgaccagcga
     5881 gctcaacgcc tttcaggcgc gctacgtggt ccgtcaccgc tggaccggcc gcgtcgactg
     5941 caaggagccg cacccgggtc gctggggcgg gccaccggcc gactggaagg gggaggtgcg
     6001 ggaggcgatc gtcgcccgcg acctcgccgc ggtcccgcgc gacgcgagcc tcgccggcta
     6061 cgtcgcgcac gacatcccgg agatccgcta cgtcgtgcgc gcggacgagc cgccggcgcg
     6121 aaagcccgcc atccccgccg cgaacccggc cccggcgccc gatgtcgagg tcgtgctgcc
     6181 cggtgagccg gtcgcctcga cgcgcaccag ctgcggctgc accggcggcg aggcgcccgc
     6241 gggctggctc gggctgctcg cattgctggc gatccggcgc cggcggccct gacgtgctcg
     6301 ccgggcggcc ggcgcatatg ttaggagcgt cgcgtgaacg aggcgaatgc gcagggcccc
     6361 gcgcggcccg gggagttcgc ggcgctgcag gtcggcccgt tgcgggtgtg gccgcccgtc
     6421 gtgctcgcgc cgatggcggg cgtcaccaac tacccgtttc gcgcgctgtg tcgccgcttc
     6481 ggcgccggcc tctacgtcag cgagatgatc accgcgcgcc cgctggtccg cggcgacgcg
     6541 cagagccggc gcctcgccga cttcggcccg ggcgaggccc cgcgcagcct ccagctgtac
     6601 ggcgtcgatc cggcggacat cggcgccgcg gtgcgctggc tcgtcggcga gggccgcgtc
     6661 gatcacgtcg acctcaactt cggctgtccg gtccgcaagg tgacccgcaa gggcggcggc
     6721 gcggcgattc ccctcaagcg caacctcctc cgcgcgatcg tccgcgccgc ggtgcaggcc
     6781 gcgggcgcgg tgccggtgac gatgaagttt cgcatcggcg tcgacgatgc gctgacgacc
     6841 ttcgagaccg cggggaagat cggcgaggac gagggctgcg ccgcggtcgg cctgcacgcc
     6901 cgcacggccg cgcagggcta ttcgggcacg gcccggtggg cggcgatcgc acggctgaag
     6961 caacacgtca cgaggatccc cgtgctcggc aacggggaca tctgggaggc gagcgacgcc
     7021 ctgcggatga tgcgagagac cggctgcgac ggcgtcatcg tcggccgtgg ctgcctcggt
     7081 cggccgtggc tgtttcgcga tctcaacgac gtttttcacg gacgtgagcc gcgcaatccg
     7141 cccacgctgg gcgaggtggt cgacatcatg atcacgcacg cgcg
//
LOCUS       DASWNJ010001842         2779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_597825, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001842 DASWNJ010000000
VERSION     DASWNJ010001842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597825"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="VGB85_30810"
     CDS             <1..1107
                     /locus_tag="VGB85_30810"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0138529.1"
                     /translation="DRLEEKLGHEGSATAAITFDRTPAHLLGARGQGFSQMLMLMNNA
                     RVGVGFESIGLCEAAYRLAKAYAAERRSMGKTIDKHEMIADYLDEMRTDIQGLRALAM
                     HGGIQEELSQRLKMQANLDSEPDSIERARLLKQSRRHAHKARKVTPLLKYLAAEKAVE
                     MARRCVQIHGGSGYIKEYGAEKLLRDAMVMPIYEGTSQIQSLMAMKDAMSNIMRNPQK
                     FLTRVAQSRWRSLSALDPLERRVARLTALSLSAQQHLIQRTATDKLRSLGKHKVREWS
                     KEFFQNWEPKRDFAYAMLHAERLTRLLADVAISEILLGQARQHPERRDVLERYLDRAE
                     PRCTFLHDEITTRGDRLLATLREQQGEESTAERA"
     gene            1136..2029
                     /locus_tag="VGB85_30815"
     CDS             1136..2029
                     /locus_tag="VGB85_30815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138530.1"
                     /translation="MPPAAPVPKHLAPATGRAGQVDEPAVAGDVTSANEPATSASTPE
                     PATRPGKRVQGGVATAELTLSARVGENAELFAQILALHVPSGSLIADVTYGLGAFWKK
                     VAPGSYRVLASDIALKPDLPVPLGFTLRDAVDCRALPHTDASLDALVLDPPYMEGMYR
                     REVAHMAGSGTHAAFRRAYSQSAATPPADDAPRWHEAVIDLYARAGREAARALRPGGV
                     FIVKCQDEVSANTQRLTHVELITGYESLGFYCKDLFVLVRANSPAVSRLKTQVHARKN
                     HSYFLVFSKQRARHASARSFA"
     gene            complement(2039..2458)
                     /locus_tag="VGB85_30820"
     CDS             complement(2039..2458)
                     /locus_tag="VGB85_30820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138531.1"
                     /translation="MLLAPGVQAAKPVTPVVVAATMIDLEVAELGKDGARRATMLTLS
                     LPDRNGRGGPSAAELKTRVTGERGEFAEYDAKVMPEDTTAGTRYVIELRRSGNDANKG
                     FDMKVEVARVLKAGAPVQISRVTRPDGSGMIVTATVR"
BASE COUNT          442 a         1086 c          861 g          390 t
ORIGIN      
        1 gaccgcctcg aggagaagct cggccacgag ggctccgcca ccgccgcgat caccttcgac
       61 cgcacccccg cgcacctgct cggcgcgcgc ggccagggct tttcgcagat gctgatgctg
      121 atgaacaacg cccgcgtcgg cgtcggcttc gagtcgatcg gcctctgcga ggctgcctat
      181 cgcctggcaa aagcctacgc ggccgagcgc cgcagcatgg gcaagacgat cgacaagcac
      241 gagatgatcg ccgactacct cgacgagatg cggaccgaca tccagggcct gcgcgccctg
      301 gcgatgcacg gcgggatcca ggaggagctc agccagcgcc tcaagatgca ggccaacctc
      361 gacagcgagc ccgacagcat cgagcgcgcc cgcctgctca agcagtcccg ccgccacgcg
      421 cacaaggccc gcaaggtcac cccgctgctc aagtacctcg ccgccgagaa ggccgtcgag
      481 atggcgcgcc gctgcgtgca gatccacggc ggctccggct acatcaagga gtacggcgcc
      541 gagaagctcc tgcgcgacgc catggtgatg ccgatctacg agggcaccag ccagatccag
      601 tcgctgatgg cgatgaagga cgcgatgtcc aacatcatgc gcaacccgca gaagttcctg
      661 acgcgggtcg cccagtcgcg ctggcgctcg ctgtccgccc tcgacccgct cgagcgtcgc
      721 gtcgctcgcc tgaccgccct ctcgctctcg gcccagcagc acctcatcca gcgcaccgcc
      781 accgacaagc tgcgcagcct cggcaagcac aaggtccgcg agtggtccaa ggagttcttc
      841 caaaattggg agcccaagcg ggacttcgcc tacgcgatgc tgcacgccga gcgcctgacc
      901 cggctgctcg ccgacgtcgc gatcagcgag atcctgctcg gccaggcccg ccagcacccg
      961 gagcgccgcg acgtgctcga gcgctacctc gaccgcgccg agccgcgctg caccttcctg
     1021 cacgacgaga tcacgacccg cggcgaccgc ctgctcgcca ccctgcgtga gcagcagggc
     1081 gaggagtcga ccgccgaaag ggcgtgactc cgcgagatcc ggtaggatgc ggcccgtgcc
     1141 gcccgccgcg cccgtcccga agcacctggc ccccgcgacc ggtcgcgcgg gccaggtcga
     1201 cgagccggcc gtcgcgggtg acgtgacatc cgcgaacgag ccggccacca gcgcctcgac
     1261 ccccgagccc gcgacgcgcc ccggcaagcg cgtgcagggt ggtgtcgcca ccgcggagct
     1321 gaccctgtcc gcgcgcgtcg gcgagaacgc cgagctgttc gcccagatcc tcgcgctgca
     1381 cgtcccgtcg ggcagcctga tcgccgacgt cacctacggc ctcggcgcgt tctggaagaa
     1441 ggtcgcgccc ggcagctatc gcgtgctcgc ctcggacatc gccctcaagc ccgacctccc
     1501 ggtccccctc ggcttcaccc tgcgcgacgc cgtcgactgc cgcgccttgc cccacaccga
     1561 cgccagcctc gacgccctcg tgctcgaccc gccgtacatg gaaggcatgt accgccgcga
     1621 ggtcgcccac atggccggct cgggcaccca cgcggcgttt cgccgggcct acagccagag
     1681 cgccgccacc cctcccgccg acgacgctcc gcgctggcac gaggccgtga tcgacctcta
     1741 cgcgcgcgcc ggtcgcgagg ccgcccgcgc attgcgtccc ggcggcgtct tcatcgtcaa
     1801 gtgccaggac gaggtcagcg ccaacaccca gcgcctgacc cacgtcgagc tgatcaccgg
     1861 ctacgagtcg ctcggcttct actgcaagga cctgttcgtg ctcgtgcgcg ccaacagccc
     1921 ggccgtgtca cgcctcaaga cccaggtcca cgcgcgcaag aaccacagct acttcctcgt
     1981 gttcagcaaa caacgggccc gtcacgccag cgcccgcagc ttcgcctgat cgtcccgtct
     2041 agcggaccgt cgccgtcacg atcatcccgc tgccgtcggg ccgcgtcacc cggctgatct
     2101 gcaccggcgc gccggccttg agcacccgcg ccacctcgac cttcatgtcg aagcccttgt
     2161 tcgcgtcgtt gcccgagcgc cgcagctcga tgacgtagcg cgtgccggcg gtcgtgtcct
     2221 cgggcatcac cttcgcgtcg tactccgcga attcaccgcg ctcgccggtc acgcgtgtct
     2281 tgagctccgc cgccgacgga ccgccgcgac cgttgcgatc cggcagcgac agggtcagca
     2341 tcgtcgcccg gcgggcgccg tccttgccga gctcggcgac ctcgaggtcg atcatcgtcg
     2401 ccgcgacgac gaccggcgtc actggcttcg ccgcctgcac gcccggcgcg agcagcatga
     2461 gagagaggga cagggcagag acacggagct ttcggatcag catgcggcgc tgctgagcaa
     2521 gcgacgtgcc acgcgtgcac caccacgatc gcagcgcttc ctcgggcctg ctcgtcgcct
     2581 cacgtccgcg gttggcagcg cggcgtccca actcgtgcca tgctggcagg cccccaaacc
     2641 gcgcattcgc cgcatttctc gtccgtgtca aaatgtcatc cggccccgga cggagtgaca
     2701 agagcgcggg acaggccgtc caggtccccc ggtcaccccc tggaattgtt catctttctt
     2761 gcagttttta gcaggagcg
//
LOCUS       DASWNJ010001843         1638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_316704, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001843 DASWNJ010000000
VERSION     DASWNJ010001843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316704"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..>1638
                     /locus_tag="VGB85_30825"
     CDS             226..>1638
                     /locus_tag="VGB85_30825"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY0138532.1"
                     /translation="MAHTALAAVVLGWLAGCADPPPARDCGVTVWAQPSVSGAKLEVI
                     GSWDGWLVPGIPLEASSEPGWQVARIDGALAPGEYGYLILEDGAHRLDGHNPRTTFWA
                     EQDDLEVSLMIVPDCSLPALTVDEVEVSGDTVSVAASFATARDGSPLKSVTASSGVPV
                     KIGERGAVELTLAGLPRGKHELVLAAEDEAGRTVQARVAAFVQPAAATWADGLLYQIV
                     TDRYRGPGGAALAPPASPGSRAGGTLDGITAEIEAGTFTDMGVTALWISPVYTNPDEA
                     REGRGDGRMYEGYHGYWPAEPRGVDPKIGGEAALERMIAAAHARGLRVLLDVVPNHVY
                     ETSPRFTHRPADAWFNWAEPACVCGLGACDWGRYIQTCWFTAYLPDLRLQEPAALRTA
                     YEDLVWWRERFAVDGMRVDAVPMMPRAATRRMAHELRASVSGEAPFLIGEVFTGAGSW
                     GVDVIRYYLGADDLDGLDSVF"
BASE COUNT          207 a          503 c          679 g          249 t
ORIGIN      
        1 cgtcgggtgt cgggttgcgg ttctgaggag atgccgcgcg gacgggcgca ggatgttcgt
       61 gccagcggcg ggacgacggg gggtgggctc ggcgcatcgc cgtgtcctgc gggtcgacgc
      121 ggcgcagcag gctcgccgag agctgtcgcc cggggcgctg cgatctttaa taaacgcgag
      181 acgccgcgcg cggaatttgc caggatcacg gtgaacgtgc gtcccgtggc tcatacggcg
      241 cttgctgcgg tcgtgctcgg ctggctcgcc ggctgcgccg atccgccgcc ggcccgcgat
      301 tgcggcgtga cggtgtgggc gcagccgtcg gtgtccgggg cgaagctcga ggtgatcggg
      361 agctgggacg ggtggctggt gccggggatc ccgctcgagg catcctcaga gcccgggtgg
      421 caggtggcgc ggatcgatgg ggcgctggcg ccgggcgagt acgggtacct gatcctcgag
      481 gatggtgcgc accgcctcga cgggcacaac ccgcgcacga ccttttgggc cgagcaggat
      541 gacctcgagg tgtccctgat gatcgtgccc gactgctcgc tgccggcgtt gacggtcgat
      601 gaggtcgagg tctcgggaga tacggtgtcg gtcgcggcga gcttcgcgac ggcacgtgac
      661 ggctcaccgt tgaagtcggt gacggcgagc agcggcgtgc cggtgaagat cggggagcgc
      721 ggcgcggtcg agctgacgct cgccgggctg ccgcgcggca agcacgagtt ggtgctcgcg
      781 gcggaggacg aggccggccg cacggtgcag gcgcgggtcg ccgcgttcgt gcagccggcc
      841 gccgcgacct gggcggacgg gctgctgtat cagatcgtga ccgaccgcta tcgcgggccc
      901 gggggcgccg cactcgcgcc gccggccagc ccgggatccc gcgcgggcgg cacgctggac
      961 ggcatcacgg ccgagatcga ggcgggcacg ttcacggaca tgggggtgac ggcgctgtgg
     1021 atctcgccgg tttataccaa cccggacgag gcccgcgagg gtcgcggcga cgggcgcatg
     1081 tacgagggct atcatggcta ctggccggcc gagccgcggg gggtcgaccc gaagatcggc
     1141 ggcgaggcgg cgctggagcg catgatcgcg gcggcgcacg ctcgcgggct gcgggtgctg
     1201 ctcgacgtcg tgccgaatca tgtctacgag acgagcccgc ggttcacgca caggcccgcg
     1261 gacgcgtggt tcaactgggc cgagccggcg tgcgtgtgcg ggctcggggc gtgcgactgg
     1321 gggcggtaca tccagacctg ctggttcacc gcgtatctgc cggacctgcg gctgcaagag
     1381 ccggcggcgt tgcggacggc gtacgaggat ctggtgtggt ggcgcgagcg cttcgcggtc
     1441 gacgggatgc gcgtcgacgc ggtgccgatg atgccgcgcg cggcgacgcg gcgcatggcc
     1501 cacgagctgc gcgcgagcgt gtccggtgag gcgccgttcc tgatcggcga ggtgttcacc
     1561 ggggcgggca gctggggcgt cgatgtcatt cgttattacc tcggggccga cgatctcgac
     1621 gggctcgaca gcgtgttc
//
LOCUS       DASWNJ010001844         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_384548, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001844 DASWNJ010000000
VERSION     DASWNJ010001844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384548"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2083
                     /locus_tag="VGB85_30830"
     CDS             <1..>2083
                     /locus_tag="VGB85_30830"
                     /EC_number="6.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0034081 - polyketide synthase complex
                     [Evidence IEA];
                     GO_process: GO:0030639 - polyketide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEY0138533.1"
                     /translation="IDYGPIFRGLREVQTGDREALARLELSEPIGEHLLHPALLDAAL
                     QAIASLASASSSATYLPVSVARAVLHRPRAGARLWVHVRGDLGDATSETMTAECVLFD
                     DTGSVVAELQGLGIKRATAGALRALAGGATQRLACAVRWQPATPPSVVHDPRVWAVLY
                     DESHAAIVEALASHGLKLVRVRRGAGFVRHGAGEYTADLTTAQGLADLLAGLASADEV
                     PTDFAYLAASGEPGAFTGAHELLHLVQALSAEVRATAPGLRIFTRAALGDRGAPESLA
                     QSMLWGLARVVQREHPELRCRIVDLDAGDQAGDPAAALAVELAVEDVEEQVRWRFGQR
                     EVPRLTAVRGEPATPAIVDDATYLITGAFGALGQGVARWLADRGARHLVLVGRREPDG
                     AGARMVTELREAGVDVKTALVDITSATAVDAMIRGLAGEMPPLRGVFHLAAVLADATV
                     RGMTPERLDRVLAPKVLGAWNLHQATRDLGLTAFVMFSSIASLLGAPGQANYAAANAF
                     LDALAHHRRARGEPGLALNWGPWGADDGAAGLARNLGKEDLARWQAAGVEMLGADAGM
                     AWLGEVLDAADPQLMLLLTTRGEVRPPAPVPRLFAAISRSEVQAPTRTAARTLTDQLR
                     AASPELRASMLTRELRRLVGQVLGTDGTAIDPEVSLGEQGMDSLMSLELRNAVAAALG
                     VALPVTLVYEFP"
BASE COUNT          233 a          800 c          775 g          275 t
ORIGIN      
        1 ggatcgacta cggcccgatc tttcgcgggc tgcgcgaggt gcagaccggc gaccgcgagg
       61 ccctggcccg cctcgagctg agcgagccga tcggcgagca tctcctgcac cccgcgctcc
      121 tcgacgccgc gctgcaggcg atcgccagcc tggcgagcgc gtcgtcctcc gcgacctact
      181 tgccggtcag cgtcgcccgg gccgtgctgc accggccgcg cgcgggcgcc cggttgtggg
      241 tccacgtccg cggcgacctc ggcgacgcga cgagcgagac gatgaccgcc gagtgcgtgt
      301 tgttcgacga caccggctcg gtggtcgcgg agctgcaagg cctcgggatc aagcgggcga
      361 ccgcgggggc cctgcgcgcg ctcgccggcg gcgccaccca gcgcctggcc tgcgcggtgc
      421 gctggcagcc cgcgaccccg ccgagcgtcg tgcacgaccc gcgggtctgg gccgtgctgt
      481 acgacgagtc ccacgcggcc atcgtcgagg cgctcgccag ccacggcctg aagctggtcc
      541 gggtccgtcg cggcgccggc ttcgtgcggc acggcgcggg cgagtacacc gccgatctca
      601 cgaccgcgca ggggctcgcc gacctgctcg ccgggctcgc cagcgcggac gaggtgccga
      661 ccgacttcgc gtacctcgcc gcctctggcg agcccggggc gttcaccggg gcgcacgagc
      721 tcctgcacct cgtgcaggct ctgagcgcag aggtccgcgc caccgcgccg ggcctgcgga
      781 tcttcacccg ggctgccctc ggcgaccgcg gcgcgcccga gtcgctcgcg cagtcgatgt
      841 tgtgggggct ggcccgcgtc gtccagcgcg agcaccccga gctgcgctgc cgcatcgtcg
      901 acctcgatgc cggcgatcaa gccggagatc cagccgccgc cctcgccgtc gagcttgcgg
      961 tcgaggacgt cgaggagcag gtccgctggc ggttcggtca acgcgaggtc ccgcgtctga
     1021 ccgccgtgcg cggcgagccg gcgaccccgg cgatcgtcga cgacgccacc tatctgatca
     1081 cgggcgcctt cggggcgctc ggtcagggcg tcgctcgctg gctcgcggat cgcggcgctc
     1141 gccacctcgt gctggtcggc cggcgtgagc ccgacggcgc cggggcgcgc atggtcacgg
     1201 agctgcgcga ggccggtgtc gacgtgaaga ccgcgctcgt cgacatcacc agcgcgaccg
     1261 cggtcgacgc gatgatccgc gggctcgcgg gcgagatgcc gccgctgcgc ggcgtgttcc
     1321 acctcgcggc cgtgctcgcc gacgccaccg tgcgcggcat gacgccggag cggctcgacc
     1381 gcgtcctggc gccgaaggtc ctcggcgcgt ggaacctgca tcaggcgacg cgtgacctcg
     1441 gcctgaccgc cttcgtgatg ttctcctcga tcgcctcgct gctcggcgcg cccgggcagg
     1501 ccaactacgc cgccgccaac gcgttcctcg acgcgctggc gcatcaccgc cgggctcgcg
     1561 gggagccggg gctggcgctc aactggggcc cgtggggcgc ggacgacggc gccgccgggc
     1621 tcgcgcgcaa cctcggcaag gaagatctgg cgcgctggca ggccgcgggc gtcgagatgc
     1681 tcggcgccga cgcgggcatg gcctggctcg gcgaggtcct cgacgccgcg gatccgcagc
     1741 tcatgctgct gctgacgacc cgcggcgagg tgcgcccgcc ggccccggta ccccgcctgt
     1801 tcgccgcgat ctctcgcagc gaggtgcagg cgccgacgcg caccgccgcg cgcacgctga
     1861 ccgaccagct gcgggcggcc tcgccggagc tgcgcgcgtc gatgctgacc cgcgagctgc
     1921 ggcggctggt cggccaggtc ctcggcaccg acggcacggc gatcgacccc gaggtctcgc
     1981 tcggcgagca gggcatggac tcgctgatgt cgctcgagct gcgcaacgcc gtcgccgccg
     2041 cgctcggcgt cgcgctgccg gtgacgctgg tctacgagtt ccc
//
LOCUS       DASWNJ010001845         3141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_384562, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001845 DASWNJ010000000
VERSION     DASWNJ010001845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384562"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="VGB85_30835"
     CDS             complement(<1..895)
                     /locus_tag="VGB85_30835"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HEY0138534.1"
                     /translation="MHDPWTRAIPWGDAPVVVTGLGYGDEGKGATVDALVRRLGIELV
                     VRFNGGPQAAHHVVRDDGVVHCFAQFGSGTLVPGVRTHLGRDMLIEPLALGREAAALA
                     KIGIAAPLERVTIDLACAVVTPFHRLIGRMEELARGTGRHGSCGMGVGAAYVDAMNPR
                     VPTLRFADLRGSTEALRSKLRLIQMIKLDRAEKLVELRPHDPGLMLGLAEIGRPDLVE
                     DTIDAYAPVVGAVAVDDGEHLRAVLRDRPQHVLFEGAQGVLLDARRGFWPYVTPSRTI
                     AASAEALVTEAGAPPPLRLGVL"
     gene            complement(898..2352)
                     /locus_tag="VGB85_30840"
     CDS             complement(898..2352)
                     /locus_tag="VGB85_30840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138535.1"
                     /translation="MFTYRGYAGAPSPTAQRTVHPDLNPHGKVRECMNLTPIVVAMDV
                     TRSRGDDSRVIYAKLPMFIGQIDLRGYVPGPAISFAAIGDATSGDRAPLQVGQFEADN
                     RLDAVLSKIWLEEGGGGSGQESYELAAYYYARHTRLAAHERGEKGFFFFLGDEGFYPQ
                     VNGDQVRRILGHAPPEGIARGDHGEPLPYDLSVDSAQIFAELQDKFHTFLIFPQKSLA
                     ERRRDIDAEIKQRVEAAGGRYEGVDIRASLVWNNRNDLDIHVIAPSGEHIFYAHKRSA
                     CGGWLDVDMNVRGETTKPVENVQWTRGGAPAGRYRVFLHNYRFHEPVAPTSYRVEIEI
                     NGQIQHFEGTIGAKGETGVASEIMILEFDYDPSQRQPEDPAVLTTGPYANYADEVVLR
                     QWQSVLPAQNILKIEDPRAIVDVMLGVMAIVGGGVDLDTFLAETGQDQLDLRRDQAIH
                     TLGPLAAATRMAKARVDGDLYVGDERPASRLPRL"
     gene            2562..>3141
                     /locus_tag="VGB85_30845"
     CDS             2562..>3141
                     /locus_tag="VGB85_30845"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY0138536.1"
                     /translation="MRYTDEQRAILEHPAGSHAVVRAVPGSGKTTTLVGRVLRLCEEG
                     SAPGRIRVVMFNKSIQRSFEQRLLDAEVGGVRVTTFDALGHELLRIADRRGLLSAPLE
                     FVPDGTTTWAREVFRASLTVREALEGPEEIADAVAFWKAQLIPPTRAACPERPALLRA
                     YQEVEALRCAGGVLRVAFEDLVYTAAAVARRHP"
BASE COUNT          427 a         1107 c         1142 g          465 t
ORIGIN      
        1 gcagcacccc gaggcgcagc ggcggcgggg ccccggcctc cgtcaccagc gcctcggcgg
       61 aggcggcgat cgtgcgcgac ggcgtgacgt acggccagaa cccgcgccgg gcgtcgagca
      121 gcaccccctg cgcgccctcg aagaggacgt gctgcggcct gtcccgaagg acagcacgca
      181 ggtgctcgcc gtcgtcgacc gccaccgcgc cgacgaccgg cgcgtaggcg tcgatcgtgt
      241 cctcgacgag atcgggccgg cctatctcgg cgagcccgag catcagcccc gggtcgtggg
      301 gtcgcagctc gacgagcttc tccgcgcgat cgagcttgat catctggatc agccggagct
      361 tggagcgcag cgcctcggtc gacccgcgca ggtcggcgaa gcgcagcgtc ggcacgcggg
      421 ggttcatggc gtcgacgtag gccgcgccga cgcccatgcc gcagctgccg tgccgacctg
      481 tcccgcgggc cagctcctcc atgcgaccga tcaagcggtg aaacggggtg acgacggcgc
      541 aggcgaggtc gatcgtgacg cgctccagcg gcgccgcgat accgatctta gcgagcgcgg
      601 ccgcctcgcg cccgagcgcg agcggctcga tcaacatgtc gcggccgagg tgtgtgcgca
      661 cgccgggcac cagggtgccg ctgccgaact gggcgaagca gtgcacgacg ccgtcgtccc
      721 ggacgacgtg gtgggccgcc tgcgggccgc cgttgaagcg cacgaccagc tcgatcccga
      781 ggcgccggac cagcgcgtcg acggtcgcgc ccttgccctc gtcgccgtag ccgaggccgg
      841 tgacgacgac cggcgcgtcg ccccagggaa ttgcgcgcgt ccaggggtcg tgcatgatca
      901 cagccgcggc aggcgcgagg ccggtcgctc gtcgccgacg tacaggtcac catcgacccg
      961 cgcctttgcc atgcgggtcg ccgccgcgag cggcccgagg gtgtggatcg cctggtccct
     1021 gcgcaggtcg agctgatcct ggccggtctc cgcgaggaag gtgtcgaggt cgacgccgcc
     1081 gccgacgatc gccatcacgc cgagcatgac gtcgacgatc gcccgcgggt cctcgatctt
     1141 gaggatgttc tgcgccggca gcaccgactg ccactggcgc agcacgacct cgtcggcgta
     1201 gttggcgtag ggcccggtgg tcagcaccgc cggatcctcg ggctggcgct ggctcggatc
     1261 gtagtcgaac tcgaggatca tgatctcgct ggcgacgccg gtctcgccct tggcaccgat
     1321 cgtgccctcg aagtgttgga tctggccgtt gatctcgatc tcgacgcggt acgaggtcgg
     1381 cgcgaccggc tcgtggaacc ggtagttgtg caggaacacc cggtagcggc ccgcaggcgc
     1441 cccgccgcgg gtccactgca cgttctcgac cggcttggtc gtctcgccgc gcacgttcat
     1501 gtcgacgtcg agccagccgc cgcaggccga gcgcttgtgg gcgtagaaga tgtgctcgcc
     1561 cgacggcgcg atcacgtgga tgtcgaggtc gttgcggttg ttccacacca gcgaggcgcg
     1621 gatgtcgacg ccctcgtagc gcccgcccgc ggcctcgacg cgttgtttga tctccgcgtc
     1681 gatgtcgcgg cgccgctcgg ccagcgactt ctgcgggaag atcagaaagg tgtgaaactt
     1741 gtcctgcagc tcggcgaaga tctgcgccga gtcgacgctg agatcgtatg gcagcggctc
     1801 gccgtggtcg ccgcgcgcga tgccctcggg cggcgcatgg ccgaggatcc gacggacctg
     1861 gtcgccgttg acctggggat aaaagccctc gtcgccgagg aagaagaaga agcccttctc
     1921 gccgcgctcg tgggccgcga gccgggtgtg ccgcgcgtag tagtaggccg cgagctcgta
     1981 ggactcctgg cccgagccgc cgccgccctc ctcgagccag atcttgctca acacggcgtc
     2041 gaggcggttg tcggcctcga actggccgac ctgcaagggc gcacgatcac cggaggtcgc
     2101 gtcgccgatc gccgcgaagc tgatcgccgg tcccggcacg tagccacgca gatcgatctg
     2161 gccgatgaac atcggcagct tggcgtagat gacccgcgag tcgtcgccac gcgagcgcgt
     2221 gacgtccatc gccacgacga tcggcgtcag gttcatgcac tcgcgcacct tgccgtgcgg
     2281 gttgaggtcc gggtgcacgg tgcgctgcgc cgtcggcgac ggtgcacccg cgtaaccacg
     2341 gtatgtgaac acgtcccggt tggtcgaccg ctccccgcgt gcgacctcgc catcgtaact
     2401 tccgccgccc atcgctgcct cctcgtccct gcccgggcgt tcgcccgcgg cgccgtgacg
     2461 gtagcgggct ttgtccgcct gctcaacgca cgcgggcgcg ccgggccggg cgtgcgcggg
     2521 cgctggcgcg cgggctcgca tccgctccat actccgcggc catgcgctac acggacgagc
     2581 agcgcgccat cctcgagcac cctgcggggt cgcacgcggt cgtgcgcgcc gtcccgggct
     2641 cgggcaagac gacgacgctc gtcggccgcg tgctgcgcct ttgcgaagag ggcagcgcgc
     2701 cgggacggat ccgcgtcgtg atgttcaaca agtcgatcca gcggagcttc gagcagcgcc
     2761 tgctggacgc cgaggtcggc ggcgtgcgcg tgacgacctt cgacgcgctg ggccacgagc
     2821 tgctgcggat cgccgatcgc cgcgggctcc tgagcgcgcc gctcgagttc gtgccggacg
     2881 ggacgacgac gtgggcaaga gaggtgttcc gcgccagctt gaccgtccgc gaggcgctcg
     2941 agggcccgga ggagatcgcc gacgcggtcg cgttctggaa ggcgcagctg atcccgccga
     3001 cgcgcgcggc atgcccggag cgcccggcgc tgctgcgggc ctatcaggag gtcgaggcgc
     3061 tgcgctgcgc gggcggggtg ctgcgcgtcg ccttcgagga tctggtgtac acggcggccg
     3121 cggtggcgcg ccggcacccg c
//
LOCUS       DASWNJ010001846         3934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_68010, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001846 DASWNJ010000000
VERSION     DASWNJ010001846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68010"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VGB85_30850"
     CDS             <1..423
                     /locus_tag="VGB85_30850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138537.1"
                     /translation="LAFLIAIHPFFVDATSGPEPGPAPTLHARVVVETSTLREAGAGP
                     AIASRVRSSAAAVLRAVAIAEDGGPDDPVVAVTVRPFPGKAIGYTSTISVLREGRQLA
                     RSRQRCALCTEGELVATVERSLTQLAPRLRQLAAPAAQ"
     gene            487..1644
                     /locus_tag="VGB85_30855"
     CDS             487..1644
                     /locus_tag="VGB85_30855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0138538.1"
                     /translation="MDDDDDLRRALARLERRERHRREHSSLASIELDAAGEIVAWNRE
                     AEQLFGWTAAEIVGRSYSLLVPEASRAPDEVAALLHGDRRHINVRKDGRTIACEWSVA
                     ALEHDGVVEVHCEVHDVSAAEVQRQRQHFMQALLDRSPLGIFAKHPDGRYLYANEEFA
                     RSVGLTPTEVIGRDDFAIFHPDIAASLRRHDAELLAADVPMKREDAGVGPDSGRTYWS
                     LKFPLREDGGELLAICGIVNDITSLRQAEQERSALQQQVIASQRQALAELSTPLMPVA
                     EGVLVMPLIGGIDRRRAEQIMEALLTAVVSQRARTVVVDITGVHSVDTEVAQALVAAA
                     TGVRLLGSRVILSGIKPAVAQTLVDLGVDLGGLVTLADLRTAVARALQRGR"
     gene            1753..2688
                     /locus_tag="VGB85_30860"
     CDS             1753..2688
                     /locus_tag="VGB85_30860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138539.1"
                     /translation="MSALHRTAGAATLLLCCACARPTEVELRLHACALAGQVPVKVDL
                     EIRGFDVGGTRLPVLRDSFPIDPEALADGYATVALVKPEGMVEADFTISWHDGRGEVE
                     VVELLDRPVPAAGQVLELGADHCMPLDPGTSSSSGEDTSTSSSSTTDASSSSSGDDTS
                     TTGDTTSSTGDTDTGGTTGEPSMAGTPCNGGGGMDQFYCEHGGPGQLGTMLHCLNNKW
                     EYGDLPDVCESLFKFNCPDSLGLVDPVLVGCSGLGPNGLTCVCRDMAPQPCQQTGCGP
                     GETITLCDDGERAQAVCTNYCEMFDGEPMCVADMP"
     gene            2685..2990
                     /locus_tag="VGB85_30865"
     CDS             2685..2990
                     /locus_tag="VGB85_30865"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEY0138540.1"
                     /translation="MTAPIDLAHHVRDNVLEIVWEDGLRARLAVPVLRGWCPCATCQG
                     HAPGIRFKDHGEAITLVDAAEVGAYALGIRFSDGHDSGIYTWAFLRQLAEGRDPSRM"
     gene            3018..>3934
                     /locus_tag="VGB85_30870"
     CDS             3018..>3934
                     /locus_tag="VGB85_30870"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0138541.1"
                     /translation="MDEALTDSLTEATRLAAESLAARNAGDLALALERAEACLAVRTV
                     ALGEQHADTAAALGLVAAARRRRGELDAALAADLRALEIYKDVLGDSHADTAVALGNL
                     SATRRARGEHDEALTLATRALAGLQAACEANDLRIALAWGNLASVHRAREEHPQAIAG
                     ARALLAIRAAVLGERHPDTAAGHSLLGSMLAAAGDHAAAAVEEQAALTIREAVSDPRA
                     TLESLINLATIHRNLGDAARSLDLQRQALEIQRRLLGEADPATTRSRLYVAYTMLRLG
                     RRREAEQLIAEGLRHDPRSKDLRQLKQQL"
BASE COUNT          547 a         1373 c         1456 g          558 t
ORIGIN      
        1 ctcgctttcc tgatcgccat tcatccattc ttcgtcgacg caacctccgg gcctgagccc
       61 ggccccgcgc ccaccctgca cgcgcgtgtc gtggtcgaga cctcgacgct gcgcgaagcc
      121 ggagccggac ctgcgatcgc ctcgcgtgta cgtagttccg cggccgcggt gttgcgtgcc
      181 gtggcgatcg ccgaggacgg cggccccgac gacccggtcg tcgccgtcac cgtgcgcccg
      241 ttcccgggca aggcgatcgg ctacacctcg acgatctcgg tgctccgcga gggccgacag
      301 ctggcccgca gccgccagcg ctgtgccctg tgcaccgagg gcgagctggt ggccacggtc
      361 gagcgctcgc tgacgcagct ggccccgcga ttgcgccagc tcgctgctcc tgcggctcaa
      421 tagcgtctcg cgcgggtgcc cggtatccgc ggaccagggc ttgcgatatg gtgatcgtgg
      481 gcccgcatgg acgacgacga cgatctgcgg cgcgcgctgg cgcggctgga gcgacgtgag
      541 cggcatcgac gtgagcactc gtcgctcgcg tcgatcgagc tcgacgccgc cggtgagatc
      601 gtcgcgtgga accgcgaggc cgagcagctg ttcggctgga ccgcggcgga gatcgtcggg
      661 cgcagctaca gcctgctggt gccggaggcg tcgcgggctc cggacgaggt cgcggcgctg
      721 ctgcacggtg atcgccgtca catcaacgtt cgcaaggacg gccggacgat cgcctgcgag
      781 tggtcggtcg ctgcgctcga gcacgacggg gtcgtcgagg tccactgcga ggtgcatgac
      841 gtcagcgcgg ccgaggtgca gcgccagcgc cagcacttca tgcaggcgct gttggatcgc
      901 tcgccgctcg gcatcttcgc caagcacccg gatggccgtt atctgtacgc caacgaggag
      961 ttcgcgcgca gcgtcgggct gacgcccacc gaggtgatcg gccgcgacga ctttgcgatc
     1021 tttcacccgg acatcgcggc gtcgctgcgt cggcacgacg cggagctgct cgccgcggac
     1081 gtgccgatga agcgcgagga cgccggcgtc gggcccgaca gcgggcgcac ctactggtcg
     1141 ctcaagttcc cgctgcgcga ggatggaggc gagctgctgg cgatctgcgg gatcgtcaac
     1201 gacatcacct cgctgcggca ggccgagcag gagcgcagcg cgctgcaaca gcaggtgatc
     1261 gcgtcgcagc gccaggcgct cgccgagctg tcgacgccgc tgatgccggt cgccgagggc
     1321 gtgctcgtga tgccgctgat cggcggcatc gaccgccgcc gggccgagca gatcatggag
     1381 gcgctgctga ccgcggtggt ctcccagcgc gcgcgcacgg tcgtcgtcga catcaccggg
     1441 gtgcacagcg tcgacaccga ggtcgcgcag gcgctcgtcg cggcggcgac gggggtccgc
     1501 ctgctcggct ccagggtcat cctgtccggc atcaagccgg cggtcgcgca gacgctggtc
     1561 gacctcggcg tcgacctcgg cggcctcgtg accctcgccg acctgcgcac cgcggtcgcc
     1621 cgggccctcc agcgcggccg ctgacgggca ccggcatact ccaggtgttg ccttgcgctg
     1681 ggcgtatgtt cagctttccg ccacacgcgc gcttacggcc gagcctgggc attttctgat
     1741 agcctccgcg cggtgagtgc actgcatcgc accgcgggcg ccgcgacctt gctcctgtgc
     1801 tgcgcgtgcg cgcgaccgac ggaggtcgag ctgcgcttgc acgcgtgcgc gctggcgggc
     1861 caggtgccgg tcaaggtcga cctcgagatc cgcgggttcg acgtaggggg cacgcggctg
     1921 cctgtccttc gggacagctt cccgatcgat cccgaggcgc tcgccgacgg ctacgcgacg
     1981 gtcgccctgg tcaagcccga gggcatggtc gaggccgatt tcacgatctc gtggcacgac
     2041 ggacgcggcg aggtcgaggt ggtcgagctg ctcgatcgcc cggtgcccgc ggctggtcag
     2101 gtgctcgagc tcggcgcgga ccactgcatg ccgctcgacc cggggaccag cagctcgtcg
     2161 ggcgaggaca cgagcaccag cagctcgtcg acgacggacg cgtcgagcag ctcgtccgga
     2221 gacgacacga gcacgacggg tgacacgacg tcgtcgacgg gtgacacgga caccgggggc
     2281 acgacgggcg agccgagcat ggccggcacg ccctgtaacg gcggcggtgg catggaccag
     2341 ttctactgcg agcacggcgg cccgggccag ctcgggacga tgctgcactg cctcaacaac
     2401 aaatgggagt acggcgacct cccggatgtg tgcgagtccc tgtttaaatt caactgtccg
     2461 gactcgctcg gcctggtgga cccggtgctg gtcggttgca gcggcttggg gccgaacggt
     2521 ctgacctgcg tgtgcaggga catggcgccg cagccgtgcc agcagaccgg gtgcggaccc
     2581 ggcgagacga tcacgctgtg cgacgacggc gagcgggcgc aggcggtctg cacgaactac
     2641 tgcgagatgt tcgacggcga gccgatgtgc gtggcggata tgccatgacg gcgccgatcg
     2701 acctcgcgca ccacgttcgc gacaacgtgc tcgagatcgt ctgggaagac gggctgcgcg
     2761 cgcgcctcgc ggtgccggtg ctgcgcggct ggtgcccatg cgcgacctgc cagggccacg
     2821 cgccggggat ccggttcaag gaccacggcg aggcgatcac cctggtcgac gcggcggagg
     2881 tcggcgccta cgcgctcggc atccgcttct cggacggcca cgactcgggg atctacacgt
     2941 gggcgttcct gcggcagctc gccgagggcc gcgatccctc gcggatgtag ggcggcgctc
     3001 gtgtatatcg gtgggccatg gacgaggcgc tgaccgactc cctcaccgag gccacgcgcc
     3061 tcgccgccga gagcctcgcg gcgcgtaacg ccggtgacct cgccctcgcc ctcgagcgcg
     3121 ccgaggcgtg cctggccgtg cgcacggtgg cgctcggtga gcagcacgcc gacaccgccg
     3181 ccgcgctcgg cctggtcgcg gcggcgcggc ggcgcagagg cgagctcgac gcggcgctcg
     3241 ccgccgacct gcgcgcgctc gaaatttata aagatgtcct cggggacagc cacgccgaca
     3301 ccgcggtcgc gctcggcaac ctcagcgcga cgaggcgggc ccgcggcgag cacgacgagg
     3361 cgctgacgct ggcgacgcgc gcgctggcgg ggttacaggc ggcgtgcgag gcgaacgacc
     3421 tgcggatcgc gctggcgtgg gggaacctcg cgtcggtgca ccgggcccgc gaggagcatc
     3481 cgcaggcgat cgcgggcgcg cgcgcgctgc tggcgatccg cgcggctgtg ctcggggagc
     3541 gccaccccga cacggcggcc gggcacagcc tgctcggctc gatgctcgcg gcggccggtg
     3601 accacgcggc ggcggcggtc gaggagcagg cggcgctgac gatccgcgag gcggtcagcg
     3661 atccgcgggc gacgctcgag tcgctgatca acctggcgac gatccaccgc aatctcggcg
     3721 acgcggcgcg ctcgctcgat ctgcagcgac aggcgctgga gatccagcgc aggctgctcg
     3781 gcgaggccga cccggcgacc acgcgttcgc ggctgtacgt cgcatacacg atgctgcgcc
     3841 tcggtcgccg ccgcgaggct gagcagctga tcgccgaggg cctgcgtcac gacccgcgca
     3901 gcaaggacct gcgccagctc aagcagcagc tcga
//
LOCUS       DASWNJ010001847         1559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_211297, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001847 DASWNJ010000000
VERSION     DASWNJ010001847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211297"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VGB85_30875"
     CDS             complement(<1..283)
                     /locus_tag="VGB85_30875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138542.1"
                     /translation="MHMHVAEDALATIKRPSAGPGPAAADLLGDLKAKMKGGGKWMDW
                     VWIGGATIVVLTVMLGSFTSIQPGEVAVRVNNLTGAQLTVTQPGLVTRLP"
     gene            complement(353..>1559)
                     /locus_tag="VGB85_30880"
     CDS             complement(353..>1559)
                     /locus_tag="VGB85_30880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138543.1"
                     /translation="ELHRLTPRVRAAAGVLMTRRFVKADELSVDIKASDGRRLVKIRA
                     LASGDNQIAGWLASFIDPGQPEMSGVFPVAREPSGIYPAMPVMPVMPVAREPSGMLPA
                     ASMMSMSSPTMTSMTAAPSMTSMPSMTAAPREPSGVYPAPLAHESGVMPMASAVHSMP
                     GMSQSLASAEASGVRPVSRHSSGALSMARSGPLRAAREANEERDAAASEAALAVNVAM
                     LTDPEMPLPSFPEPPATPREAPPASAPQLDAAAGVDPLDRLTRLAGLETVDPRDANEP
                     AAPVALAGSDALGVPPGPDAPIASATDPRVASVAAPPSSQPLAPPVVWSSNAAPPAVA
                     LAGLASASTSSETPSTPPELPPRAPPATAPTFAPPALPRPPVIARPPASEPTASATPE
                     PPRDPDETR"
BASE COUNT          188 a          581 c          587 g          203 t
ORIGIN      
        1 acggcagccg cgtgaccagg cccggctgcg tcaccgtcag ctgcgccccg gtcaggttgt
       61 tgacgcgaac cgccacctcg ccgggctgga tcgaggtgaa cgacccgagc atcaccgtca
      121 gcaccacgat cgtggcgccg ccgatccaca cccaatccat ccacttgccg ccgcccttca
      181 tcttcgcctt gaggtcgccg agcaggtccg ccgcggccgg ccccgggccc gcgctgggtc
      241 gttttatcgt cgccaatgca tcctccgcga cgtgcatgtg cacgccacgg acgcatccta
      301 caccagcagg agccggcctc gacggccccg gccgggcccg gtgcgcgcag gtctagcggg
      361 tctcgtccgg atcgcgcgga ggctccggcg tcgcggatgc ggtcggctcg gatgcgggcg
      421 gccgcgcgat cacgggcggg cgcggtagcg cgggcggggc gaaggtcggc gccgtcgcgg
      481 gcggtgccct cgggggcagc tccggcggcg tgctcggcgt ctccgacgag gtcgacgcgc
      541 tcgccagacc cgcaagcgcg accgccggtg gcgcagcgtt ggacgaccag accacgggcg
      601 gtgcgagcgg ttgcgacgac ggcggcgcag cgaccgaagc gaccctcggg tccgtagcgg
      661 acgcgatcgg ggcgtcgggc cccgggggca cgccgagcgc gtcggagccg gcgagcgcga
      721 cgggtgcggc gggctcgttg gcgtcgcgcg ggtcgaccgt ctcgagacct gcgagcctcg
      781 tcagccggtc gagggggtcg acgccggcgg ccgcgtcgag ctgcggcgcg ctcgcggggg
      841 gcgcctcgcg aggtgtcgcc ggcggctccg ggaagctcgg cagcggcatc tccgggtcgg
      901 tgagcatcgc cacgttgacc gccagcgccg cctccgaggc cgcggcgtcg cgctcctcgt
      961 tggcctcgcg agccgcgcgc agcgggccgc tgcgcgccat cgacagcgcg ccgctgctgt
     1021 gccgcgacac cggccgcacg ccgctcgcct cggcgctggc gaggctctgc gacatgcccg
     1081 gcatgctgtg caccgcggat gccatcggca tcacgccgct ctcgtgcgcc agcggtgccg
     1141 ggtacacgcc gctcggctcg cgcggcgccg ctgtcatcga cggcatcgat gtcatcgacg
     1201 gcgccgccgt catcgatgtc atcgtcggcg acgacatcga catcatcgac gcggctggca
     1261 gcatgccgct cggctcgcgc gcgaccggca tgaccggcat gacgggcatg gccgggtaga
     1321 tgccgctcgg ctcgcgcgcg accgggaaca cgccgctcat ctccggctgc ccggggtcga
     1381 tgaagctcgc cagccagccg gcgatctggt tgtcacccga ggcgagcgcc cggatcttga
     1441 ccaggcggcg gccgtcgctc gccttgatgt cgaccgagag ctcgtcggcc ttgacgaagc
     1501 ggcgcgtcat cagcacgccc gcggccgcgc gcacgcgcgg ggtcaggcga tgcagctcg
//
LOCUS       DASWNJ010001848         1529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_384669, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001848 DASWNJ010000000
VERSION     DASWNJ010001848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384669"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VGB85_30885"
     CDS             <1..699
                     /locus_tag="VGB85_30885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138544.1"
                     /translation="VYTPIAADERDDDIAGDCRVLMCDGAGAGTPVDDATDVPDDANS
                     CTQDLCSAAGPENVPQAPGSACADDGYCHADLTCQPCPERDACLDDSAAEPNETQGAA
                     DPRPQRSDADGPAYACEALGDPGDVDWFTLDTLDLAFGKVAPAVLVTPDSLQVCVYFQ
                     CKQGGTTVQCPEGSVATNAPVGQLGCCGIGQLAPQIDCKGFNEDATLWLSVRHDLDAG
                     EPVACLNYQLAYEY"
     gene            complement(718..>1529)
                     /locus_tag="VGB85_30890"
     CDS             complement(718..>1529)
                     /locus_tag="VGB85_30890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY0138545.1"
                     /translation="RVRLVGVVGGDFPTTAVGDLRDRGVDLTGLEIIPDGKTFRWKGR
                     YHDNMNDRTTLATDLNCFEQFSPKLPPDYRTSDYLFLANIQPGLQEQVFNQMQRPKLV
                     GLDTMNLWISIANAELRSVLRHVDVLTLNDEEARQLSGEHNLVKAARAVQKLGPKNLV
                     IKRGENGALLFDRDGEMFAAPALPLEEVIDPTGAGDCFAGGFMGYLARPGVEPTGASL
                     RTAMIYGSVMASFCVEGFSYDRLRSLDAATIQHRFEAFSSLTNFERANLLA"
BASE COUNT          242 a          563 c          501 g          223 t
ORIGIN      
        1 gtgtacaccc cgatcgccgc cgacgagcgc gacgacgaca tcgccggcga ctgtcgcgtg
       61 ctcatgtgcg acggcgccgg cgcgggcacc cctgtcgacg acgccaccga cgtccccgac
      121 gacgccaaca gctgcacgca ggacctgtgc agcgccgccg gccccgagaa cgtcccgcag
      181 gcccccggca gcgcctgcgc cgacgacggc tactgccacg ccgatctcac ctgccagccc
      241 tgccccgagc gcgacgcctg cctcgacgac agcgccgccg agcccaacga gacccagggc
      301 gccgccgacc cacgcccgca gcgcagcgac gccgacggcc ccgcgtacgc ctgcgaggcg
      361 ctcggagacc ccggcgacgt cgactggttc acgctcgaca ccctcgacct cgccttcggc
      421 aaggtcgccc ccgcggtctt ggtcaccccg gactccctgc aggtctgcgt ctactttcaa
      481 tgcaagcagg gcggcaccac cgtgcagtgc cccgagggca gcgtcgccac caacgccccg
      541 gtcggccagc tcggctgctg tggtatcggt cagctcgccc cgcagatcga ctgcaagggt
      601 tttaatgagg acgcgacgct gtggctcagc gtccgccacg acctggacgc gggcgagccg
      661 gtagcgtgtc tcaactacca gctcgcctac gagtactaga tcgttacgcg agcaagatca
      721 ggcgagcaag ttggcccgct cgaagttcgt cagcgacgag aacgcctcga agcggtgctg
      781 gatcgtcgcc gcgtcgaggc tgcgcaggcg gtcgtacgag aagccctcga cgcagaagct
      841 cgccatcacc gacccgtaga tcatcgcggt gcgcaggctc gcgccggtcg gctcgacgcc
      901 cgggcgcgcc aggtagccca tgaagccgcc cgcgaagcag tcgccggcgc cggtcgggtc
      961 gatgacctcc tcgagcggca gggccggcgc ggcgaacatc tcgccgtcgc ggtcgaacag
     1021 cagcgccccg ttctcgccgc gcttgatcac caggttcttg ggcccgagct tttgcaccgc
     1081 gcgcgccgcc ttgaccaggt tgtgctcgcc gctaagctgc cgcgcctcct cgtcgttgag
     1141 cgtcagcacg tcgacgtggc gcagcaccga gcgcagctcc gcgttggcga tcgagatcca
     1201 caggttcatc gtgtcgaggc cgacgagctt cggccgctgc atctgattaa aaacctgctc
     1261 ctggaggccc ggttggatgt tggcgaggaa cagataatcg ctcgtgcgat agtccggcgg
     1321 cagcttgggc gagaactgct cgaagcagtt gaggtcggtc gccagcgtcg tccggtcgtt
     1381 catgttgtcg tggtagcgac ccttccagcg gaaggtcttg ccgtccggga tgatctcgag
     1441 gccggtcagg tcgacgccgc ggtcgcgcag gtcgccgacc gccgtcgtcg ggaagtcacc
     1501 gccgacgacc ccgaccaggc gcacgcgcg
//
LOCUS       DASWNJ010001849         2489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_668902, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001849 DASWNJ010000000
VERSION     DASWNJ010001849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668902"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VGB85_30895"
     CDS             complement(<1..349)
                     /locus_tag="VGB85_30895"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEY0138546.1"
                     /translation="MTPLRSRMLPRRTVLRGLIGGAAISLPLPRLAGMLNGNGTAYAD
                     GTRLKPRFLTWFFGNGADPSQWVPAATGVGPAWSLSPSLAPLADYKPWVSVLSGYDLK
                     VPYIYAHKSSPAAI"
     gene            complement(346..2100)
                     /locus_tag="VGB85_30900"
     CDS             complement(346..2100)
                     /locus_tag="VGB85_30900"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEY0138547.1"
                     /translation="MQRSDLTLGALALAFGLASSACYDGATLDSGSATATSTGSEATA
                     ADADGGGDSGSGATGDAPPALPEVPAPTSRRLTTAEFKHSVTDLLGPVTLSAIEADAS
                     QEGFFSVGNARIALSPAGVALYEKALEAATLEAFADPARVPTIVACVPALPTDTVCLR
                     DAIASFGRRAWRRPLAAAELDRYLGIATAVGSETSDAVVGLRHAVWGLLQSPHFLYRV
                     ELGAAGADGRVAFSSYEMASRLAFTLWNTLPDEALLDAAERDELTSADGVVTQATRML
                     ADPRARQGVHNFIGELYELWLLDEKVKDPTQFPEWTPTLRQVMRDELLARIEDVVFTA
                     PDDFFALYDSPKVFVNNELAALYGLPPADPDSFRAELLPEGSPRRGLIGSGLMLAMNS
                     LPARTSATARGAFIAEALLCRTVPPPPPEVDTDLDKPDEMNPGPRTLREKLEPHRSNP
                     ACAGCHNITDPLGLALEHFDTVGRYRELDQGLVIDASGELDGMPFVDGAELASRLREH
                     PEVARCLVKKLATYTAGRLPALAEFETLAALEDALAIEGNRFDRLLFALVTHDDFRFA
                     HPPDSVIAPDHQDMGEMP"
BASE COUNT          388 a          752 c          973 g          376 t
ORIGIN      
        1 ggatcgccgc gggcgacgat ttatgggcgt agatatacgg gaccttgagg tcgtagccgg
       61 acagcaccga tacccatggc ttgtaatcgg cgagcggcgc gagcgagggg ctgagcgacc
      121 acgccgggcc gacgccggtg gcggcgggga cccactggct cgggtcggcg ccgttgccga
      181 agaaccacgt caaaaagcgc ggcttgaggc gggtgccgtc ggcgtaggcg gtgccgttgc
      241 cgttgagcat gccggcgagg cgcggcagcg gcagcgagat cgcagcgccg ccgatcaggc
      301 cgcgtaggac agtgcgacgc gggagcatcc tggagcgcag cggggtcatg gcatctctcc
      361 catgtcttga tggtcgggcg cgatcacgct gtccggcggg tgggcgaagc gaaagtcgtc
      421 gtgggtgacg agcgcgaaca gcaggcggtc gaagcggttg ccttcgatcg ccagcgcgtc
      481 ctcgagggcc gcgagggtct cgaattcggc gagcgcgggc aggcggcccg ccgtgtaggt
      541 cgcgagcttc ttgacgaggc agcgggcgac ctcggggtgc tcgcgcaggc ggctcgccag
      601 ctcggcgccg tcgacgaagg gcataccatc gagctcgccg ctggcgtcga tgacgaggcc
      661 ctggtcgagc tcgcggtagc ggccgaccgt gtcgaagtgc tcgagcgcga gaccgagcgg
      721 gtcggtgatg ttgtggcagc cggcgcaggc cgggttcgag cggtgcggct cgagcttctc
      781 acgcagggtg cgggggccgg ggttcatctc gtcgggcttg tcgaggtcgg tgtcgacctc
      841 gggcggcggc ggcgggaccg tgcgacacag cagggcctcg gcgatgaagg caccacgcgc
      901 ggtcgcggag gtgcgggccg gcagcgagtt catcgccagc atcaacccac taccgatcag
      961 gccccggcgc gggctgccct cgggcagcag ctcggcgcgg aagctgtcgg gatcggccgg
     1021 cggcaggccg tagagggccg cgagctcgtt gttcacgaac accttcgggc tgtcgtagag
     1081 ggcgaagaag tcgtccggcg cggtgaacac gacgtcctcg atgcgtgcga gcagctcgtc
     1141 gcgcatcacc tgacgcagcg tcggggtcca ctcggggaac tgcgtcgggt ccttgacctt
     1201 ctcgtcgagc aaccacagct cgtacagctc gccgatgaag ttgtgcacgc cctggcgcgc
     1261 gcgcgggtcg gcgagcatgc gggtcgcctg ggtgacgacg ccgtcggcgg aggtcagctc
     1321 gtcgcgctcg gcggcgtcga gcaaggcctc gtcggggagg gtgttccaca gggtgaaggc
     1381 gaggcgcgag gccatctcgt aggaggagaa ggcgacgcgc ccgtcggcgc cggcggcccc
     1441 gagctcgacg cgatacagga agtgcggcga ctgcagcagg ccccagacgg cgtggcgcag
     1501 gccgacgacg gcgtcgctgg tctcgctgcc gacggcggtg gcgatgccga ggtagcggtc
     1561 gagctcggcg gcggcgagcg ggcgccgcca ggcgcgacga ccgaagctgg cgatcgcgtc
     1621 gcgcaggcac acggtgtcag tcggcagggc cgggacgcag gcgacgatcg tcgggacgcg
     1681 ggccgggtcg gcgaaggcct cgagggtcgc ggcctcgagc gccttctcgt agagcgcgac
     1741 gccggcaggg gacagcgcga tgcgggcgtt gccgaccgag aagaagccct cctgcgaggc
     1801 gtcggcttcg atcgcgctca gtgtcaccgg gccgagcagg tcggtgacgc tgtgcttgaa
     1861 ctcggcggtg gtcagccggc ggctcgtcgg cgcggggacc tcgggcagcg ctggcggggc
     1921 gtcgccggtc gccccactgc cggagtcgcc gccaccgtcc gcgtctgcag cggtagcttc
     1981 actgccggtg ctcgtcgcgg tcgcggaacc gctatcgagc gtggcgccgt cgtaacaggc
     2041 cgagcttgcg agcccgaacg cgagcgcgag cgcgccgagg gtcagatcgc ttcgttgcat
     2101 ggatctccaa acacgcgcaa aacgccaggc atcttaccgg cgcgcgcggc gagctgtcaa
     2161 ggcgtgcgga cgcccgcatt cgcggcgtgg agcccccagc gatggcgttt gatagggatt
     2221 tgcggccccc ggtgggaatg cagactgtgg ggataggatg tcgataatcg gggcgcgcgc
     2281 ggcgattgac gtgagtctgt ggcgaatcgt cgggtgcgat aaatgcaatt gcattaaata
     2341 tgcctcgaat gcgcaatatc cgatcgatga tatcggcgcc tggagcggcg ataaacatgg
     2401 ttcaagctgt ccgacatgtc aggaggaata agagttattt gttgttggca gtcgattaag
     2461 gtctttacga gactctcaca tacctgtac
//
LOCUS       DASWNJ010001850         3952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_316807, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001850 DASWNJ010000000
VERSION     DASWNJ010001850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316807"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VGB85_30905"
     CDS             complement(<1..602)
                     /locus_tag="VGB85_30905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138548.1"
                     /translation="MPKLSSFQSLKVVALGLAVLGGGASGCSRGSIHETQDPEAMDPQ
                     YQAAAASPALVQPVRVESQMYATPNGAQPHGYAAPGEQAVDPEAAAMAPTAAPADPAA
                     AASGGEAIPDPADLVGYETLSDGSKVKVVTYVHTYPEPIESYPRVYWSDRWYYNVNGN
                     FVFYSPYYNSWTYYWGPPYPLVYAWNGYYPWAPYAYGYGY"
     gene            771..1712
                     /locus_tag="VGB85_30910"
     CDS             771..1712
                     /locus_tag="VGB85_30910"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0138549.1"
                     /translation="MSAEIHVLKFLTGKYKGEEFPLGPDGRQFVAGRSSEADLVLADD
                     AVSRKHARFYVERRRTWVRDLGSRNGTLINGIPIKRHCLRPGDRVAIGASLMSVQLAK
                     ASQVGAAPRAGEKKQSRARPTLDFSNRSMAGSIEEIPLAGVLQFLAASRKSGNLLVRD
                     GETSRVGRVFMRDGFITNASLEGSAPGLKPEKAVMRMLGWTKGTFSLENASAEAGDER
                     VVASIEHVLMESARQQDEIMHLSERTPVPRYTSEVTLVTPGPVRWHTLKPEQLDVVQD
                     LAEGRGWAYVLDTYAGDDLELTRMIVELRKKRVVEYE"
     gene            1828..>3952
                     /locus_tag="VGB85_30915"
     CDS             1828..>3952
                     /locus_tag="VGB85_30915"
                     /inference="COORDINATES: protein motif:HMM:NF045042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0138550.1"
                     /translation="MPTSSYRLTCPSLALFLALAGCGDSGGTSESADTTAGSSPTSIS
                     GDTTGATASTETTGNTPTQAGTMTEAGSGSESLTGTGTDSTQGTGPTTGTSLTGGETT
                     TDTTGGNLCDEAMKCSGKCCGAGELCDNGECVPDCGGPPPCGEAKECCGAGDLCYLGE
                     CVTPGPACDEQVCATKPLQNACGDGFVCDPQQKLCLPSKADPTCQYIPPPNIFKPEPL
                     FTWGKRKVIACAADTDCQVAEVCTDKVCTPTWQHWIPGADDMPNHYQCSSIPVVVDID
                     DNCVPDIIFNTYTGTNAGADGVVRAIRGDTGEKLWTITDPAYRTNSTSNLAVGDIDGD
                     GLPEILADGEGKRILAFKGDGTPLWKSDPYTGADSSGSVAIADMDGEGGPEIIFGAAI
                     FSNAGKKVYEGAVGIGRNGQGPISCIADLDGDGRPELIGGRTAYKTTGTIAANDFKGA
                     VLWTNAATDGFCGVADFDPDGQPEVVLVTQGKVLILNGLTGVTRATANIPAGGTGGPP
                     NIADFDGDSLRDIGVAGSARYTVFQFDAKTNTMSQLWTAATEDDSSQVTGSSVFDFDG
                     DGRNEVVYNDEAYIRIYPGTEPDCALNPPGPGCDKNMTDAEVLFRDRNSSRTRTEYPV
                     IADVNGDFKADIVFSTNNDSNASLVTDAGVEVFKDSLDNWVSTRPVWNQHTYHITNVG
                     LVGEIPVKEDNSWTTPMDNPYNSYRN"
BASE COUNT          674 a         1386 c         1341 g          551 t
ORIGIN      
        1 tagtagccgt agccgtaggc gtacggggcc cacgggtagt agccgttcca cgcgtagacc
       61 agcggatagg gggggcccca gtagtaggtc caggagttgt agtacgggct gtagaagacg
      121 aagttgccgt tgacgttgta gtaccaacgg tccgaccagt agacccgcgg gtagctctcg
      181 atcggctcgg ggtaggtgtg cacgtaggtg acgaccttga ccttgctgcc gtcgctgagc
      241 gtctcgtagc cgacgaggtc ggcgggatcg gggatcgcct caccacctga tgcggcggcg
      301 gccgggtcgg ccggggcagc ggtgggcgcc atcgcggccg cctcggggtc gaccgcctgc
      361 tcgcccggcg ccgcgtagcc gtgcggctgg gcgccgttcg gggtcgcgta catctggctc
      421 tcgacgcgga ccggctggac cagcgccggg ctcgcggcgg ctgcctggta ctgcgggtcc
      481 atcgcctcgg gatcttgcgt ctcgtggatg ctgccccggc tgcatccgct cgcgccgccg
      541 ccgagcaccg cgagaccgag cgcgacaacc ttcaaggact ggaacgagga gagcttgggc
      601 atggggacca ccgtgggcgt tcgagactgg caaacgtgcg agcgtgttca ccacgcagac
      661 gcggccacct tcgctcgcgt cccgagccta gcccgtttag ggggcagcgg ccatggaata
      721 ggcggcggtt gacgacctcg ccgcttggca cctatcctgc tcagggcctc atgagtgcgg
      781 aaattcacgt gttgaagttc ctgacgggga agtacaaggg cgaggaattt ccgctcgggc
      841 ccgacggccg tcaattcgtc gccggtcgca gcagcgaggc ggacctcgtg ctcgccgacg
      901 acgccgtcag ccgcaagcac gcccgcttct acgtcgagcg ccggcgcacg tgggtccgcg
      961 acctcggctc gcgcaacggc acgctgatca acggcatccc gatcaagcgc cactgcctgc
     1021 gcccgggcga ccgcgtggcg atcggcgcga gcctgatgag cgtgcagctc gccaaggcga
     1081 gccaggtcgg cgccgccccg cgcgcgggcg agaagaagca gtcccgcgcc cgcccgacgc
     1141 tcgacttctc caatcggtcc atggccggca gcatcgagga gatcccgctg gcgggggtgt
     1201 tgcagttcct cgccgcctcg cgcaagtccg gcaacctgct ggtccgtgac ggcgagacca
     1261 gccgcgtcgg ccgcgtgttc atgcgcgatg gcttcatcac caacgccagc ctcgagggca
     1321 gcgcgccggg cctcaagccg gagaaggccg tgatgcggat gctcggctgg accaagggca
     1381 ccttctcgct cgagaacgcc agcgccgagg ccggcgacga gcgggtcgtc gcgtcgatcg
     1441 agcacgtgct gatggagtcg gcccgccagc aggacgagat catgcacctc tcggagcgca
     1501 cgccggtgcc gcgctacacc agcgaggtca cgctcgtcac gcccggcccg gtccgctggc
     1561 acacgctcaa gccggagcag ctcgacgtcg tccaggacct cgccgagggc cgcggctggg
     1621 cctacgtgct cgacacctac gccggcgacg acctcgagct cacccgcatg atcgtcgagc
     1681 tacgcaagaa gcgggtcgtc gagtacgagt gagagcgacc tgtccatcgg gacaggtaag
     1741 cgagatccgc gaaccgcagg cgtgcacttg cgagcctccg cgcgtcgcgc cttcgcccgc
     1801 gggctttcgt tatgatgcca ggtctacatg ccgacctcat cgtatcgcct gacctgcccc
     1861 agcctcgcgc tgttcctcgc actcgccggc tgcggcgact ccggcggcac cagcgagtcc
     1921 gcggatacca ccgccggctc gtcaccgacg agcatctcgg gcgacaccac cggcgcgacc
     1981 gcctcgaccg agacgaccgg taacaccccg acgcaggccg gcacgatgac cgaggccggc
     2041 tcgggctcgg agtcgctgac cggcaccggc accgactcga cccagggcac cgggccgacc
     2101 accggcacga gcctgaccgg cggcgagacg accaccgata cgaccggcgg caacctgtgt
     2161 gacgaggcga tgaagtgctc gggcaagtgc tgcggcgcgg gcgagctgtg tgacaacggc
     2221 gagtgtgtgc ccgactgcgg cggcccgccg ccgtgcggcg aggccaagga gtgctgcggc
     2281 gcgggcgacc tgtgttacct cggcgagtgc gtgacgccgg gtccggcctg cgacgagcag
     2341 gtgtgcgcga ccaagccgct gcagaacgcc tgcggagacg gcttcgtgtg cgacccgcag
     2401 cagaagctgt gcttgccctc gaaggccgac ccgacctgcc agtacatccc gccgccgaac
     2461 atcttcaagc ccgagccgct gttcacgtgg ggcaagcgca aggtgatcgc gtgcgcggcg
     2521 gacacggact gtcaggtcgc ggaggtgtgc acggacaagg tgtgcacgcc gacctggcag
     2581 cactggatcc ccggcgccga cgacatgccc aatcactatc agtgctcgtc gatccccgtg
     2641 gtcgtcgaca tcgacgacaa ctgcgtgccg gacatcatct tcaacacgta caccggcacc
     2701 aacgcgggcg ccgacggcgt cgtgcgtgcg atccgcggtg acaccggcga gaagctgtgg
     2761 acgatcaccg acccggcgta ccgcaccaac agcacctcga acctcgccgt cggtgacatc
     2821 gacggggacg gcctgccgga gatcctcgcg gacggcgagg gcaagcggat cctcgcgttc
     2881 aagggcgacg gcacgccgct gtggaagtcc gacccgtaca ccggcgccga cagcagcggc
     2941 tcggtggcga tcgccgacat ggacggtgag ggcggcccgg agatcatctt cggcgccgcg
     3001 atcttcagca acgccggcaa gaaggtctac gagggcgcgg tcggcatcgg tcgcaacggt
     3061 cagggcccga tctcctgcat cgccgacctc gacggcgacg ggcggcccga gctgatcggc
     3121 gggcgcacgg cgtacaagac gaccggcacg atcgccgcca acgacttcaa gggcgccgtg
     3181 ctgtggacca acgccgcgac cgacggcttc tgcggcgtcg ccgacttcga ccccgacggc
     3241 cagcccgagg tcgtgctcgt cacccagggc aaggtcctga tcctcaacgg gctgacgggg
     3301 gtcacccgcg cgaccgccaa catccccgcc ggtggcaccg gcgggccgcc caacatcgcc
     3361 gacttcgacg gcgacagctt gcgcgacatc ggcgtcgccg gctcggcccg ctacaccgtg
     3421 ttccagttcg acgccaagac caacacgatg tcgcagctgt ggaccgcggc gaccgaggac
     3481 gactcgtcgc aggtcaccgg cagctcggtg ttcgacttcg acggtgatgg ccgcaacgag
     3541 gtcgtctaca acgacgaggc gtacatccgc atctaccccg gcaccgagcc cgactgcgcg
     3601 ctcaaccccc cgggtccggg ctgcgacaag aacatgaccg acgccgaggt gctgttccgc
     3661 gaccgcaact cgtcgcgcac gcgcaccgag tacccggtga tcgccgacgt caacggcgac
     3721 ttcaaggccg acatcgtgtt ctcgaccaac aacgactcga acgcctcgct ggtgaccgac
     3781 gccggcgtcg aggtgttcaa ggacagcctc gacaactggg tctcgacgcg cccggtgtgg
     3841 aaccagcaca cctatcacat taccaatgtc ggcctcgtcg gcgagatccc cgtcaaggag
     3901 gacaacagct ggaccacgcc gatggacaac ccgtacaaca gctatcgcaa ca
//
LOCUS       DASWNJ010001851         1539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_597943, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001851 DASWNJ010000000
VERSION     DASWNJ010001851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597943"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="VGB85_30920"
     CDS             <1..1307
                     /locus_tag="VGB85_30920"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibrinogen-like YCDxxxxGGGW domain-containing
                     protein"
                     /protein_id="HEY0138551.1"
                     /translation="QNVEQCDDGANNGAGKSCNAMCKVNVCGDGDKGPSEACDDGNQS
                     NDDTCTNVCKQASCGDGFKQPGEQCDLGVNNSNSGACTLACKTPVCGDLFVQMGAGET
                     CDDGNLSNNDACLATCKLAKCGDTFTQQGVEQCDDGNLSNADLCTNDCKSASCADTFK
                     NGAETDVDCGGGTCGKCALTKACLVGGDCLSGTCTTNKCSDPTSCKALKAGNPAAPSG
                     VYSLDPDGAGPKPSFSAYCEMTYDGGGWTLALKADGSKTTFNYDNAIWLNAALYQPQF
                     PDLDHNEAKLETWNSIAFTELLVGLENPIQNPGPLNLKLVKLGLAKTSLFALLSPNLY
                     VATAIGRNAWKSLVTGSSLQPNCNKEGINSFPNGDPTFTRARVGIASNQENDCASPDS
                     FIGIGTQGIPCGGVAMSAGNMSRCTGDNGDVDKPAFGAVFVR"
BASE COUNT          296 a          577 c          461 g          205 t
ORIGIN      
        1 agcaaaacgt cgagcagtgc gacgacgggg ccaacaacgg cgccggcaag tcctgcaatg
       61 cgatgtgcaa ggtcaacgtc tgcggcgacg gcgacaaggg ccccagcgag gcctgcgacg
      121 acggcaacca gagcaacgac gacacctgca ccaacgtctg caagcaggcc agctgcggcg
      181 acggcttcaa gcagcccggc gagcagtgtg acctcggcgt caacaacagc aactccggcg
      241 cctgcaccct cgcgtgcaag acacccgtgt gcggcgacct gttcgtgcag atgggcgccg
      301 gcgagacctg cgacgacggc aacctgtcga acaacgacgc ctgcctcgcc acctgcaagc
      361 tcgccaagtg cggcgacacc ttcacccagc agggcgtcga gcagtgcgac gacggcaacc
      421 tgagcaacgc cgacctgtgc accaacgact gcaagtccgc gagctgcgcg gacaccttta
      481 aaaatggcgc cgagaccgac gtcgactgcg gcggcggcac gtgcggcaag tgtgcgctga
      541 ccaaggcgtg cctcgtcggc ggcgactgcc tcagcggcac ctgcaccacc aacaagtgct
      601 ccgacccgac cagttgcaag gcgctcaaag ccggcaaccc ggccgcgccg agcggcgtgt
      661 acagcctcga cccggacggc gccggcccca agccgtcgtt ctcggcctac tgcgagatga
      721 catacgacgg cggcggctgg acgctcgcgc tcaaggccga cggcagcaag acgacgttca
      781 attacgacaa cgcgatctgg ctcaacgcgg ccctgtacca gccgcagttc cccgacctcg
      841 accacaacga ggccaagctc gagacctgga attcgatcgc gttcaccgag ctgctcgtcg
      901 gcctcgagaa cccgatccag aacccggggc cgctcaacct caagctcgtc aagctcgggc
      961 tcgccaagac ctcgctgttc gcccttctct cgcccaacct ctacgtcgcc accgcgatcg
     1021 gtcgcaacgc ctggaagtcg ctcgtcaccg gcagctcgct gcagcccaat tgcaacaagg
     1081 agggcatcaa cagcttcccc aacggcgacc cgaccttcac gcgcgcgcgc gtcggcatcg
     1141 cctccaacca ggagaacgac tgcgcctcgc cggactcgtt catcggcatc ggcacgcagg
     1201 gcatcccctg cggcggcgtg gcgatgtcgg ccggcaacat gtcgcgctgc accggcgaca
     1261 acggcgacgt cgacaaaccc gccttcggcg ccgtcttcgt tcgctgatac gcggacgatc
     1321 gcgccatcgc atgcggcgag tgcatggcgc gcatgtcgac ttcgaatgac cgcggcgccg
     1381 cgttcgaccg catgttgtcg acatgactcg cctcgccctt gtctccctgt cgctgacgac
     1441 cgccctcctc gccctcacgg cctgcggtga cgacggcggc gcctcgacca ccggctcgac
     1501 caccgcatcc tcgaccggcg cggacgactc gaccggcac
//
LOCUS       DASWNJ010001852         2691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_316824, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001852 DASWNJ010000000
VERSION     DASWNJ010001852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316824"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VGB85_30925"
     CDS             <1..435
                     /locus_tag="VGB85_30925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138552.1"
                     /translation="PEYPEWLARSGDPIDFSTDDSEMTLAIEWSEEVIEQLPEDLTIE
                     LSGPRLATRSFALSLASRGGGIARLTFGELQRSQAITLTAKHGDAELLLVRDQIAGDL
                     EQAMVWEHHLEELLVPAEPEEDGELIASGATPDDAHGEVAVA"
     gene            437..2062
                     /locus_tag="VGB85_30930"
     CDS             437..2062
                     /locus_tag="VGB85_30930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138553.1"
                     /translation="MDHEWIVEVKDDKGAALTNATVALVETSALAGQDWPYTAAKATH
                     AHDAGGTYKAAPPIVPVAGEWMLIVRVAGKSPVVQPLKMVVTKTDVITTPSPRTAATV
                     TFSSEVKTVATSKTRRARLGVKMFPSSELVFLSGTEYFNAGTRFRIFAENYREGLRRE
                     KLVDAGVIATLLSTDDRSRETHVPAMAGGWLKVGHKSFGPTSGIKPGTEHAPVVGQDV
                     SIVDLYKYLSQVGASEPGRVKEVGIFSHSWPGGPLLFNTSDNSLTSARDIDDFDGRRK
                     DFNATNVAGWPKMKAAMAPGGTWHVWGCSATTHHKNLTVAAHKHKKDGDTAFFTVTTS
                     VQHHGGATASTMEERTNRARIRFDMDQRFRSLSYMAAAVSHLAIPVFGAPPGVGSSFD
                     QKRSIMFIDTASYANLFAYFKDVFGPEFTPTSSTYDRGYVDYQKVGGRPAPPAPGFSS
                     EAYIFNKDFDNKKTTLSVDSKKTVTMAGTAITLKVIPKTGFATPGKSGHQFELHDTAD
                     KTRSRAIYVQDDKQTFNVKLDGAGKFTVLGATL"
     gene            complement(2037..>2691)
                     /locus_tag="VGB85_30935"
     CDS             complement(2037..>2691)
                     /locus_tag="VGB85_30935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138554.1"
                     /translation="GAVAAWARGAVVHIALCRLELRDGRGEGPEAPPDPGAPLAVIAG
                     LVRDGRHVRADSSEALRVAVSDALAGLADAPADVALVVDGRGKMGQSLAGLGALQPAL
                     DRFVATAGHRVALLRWDGDGASVVAGLTQAPGVVPGALRRLRAGSPDDRPRPIFAALA
                     AAQGLPWDPAARRLVLVLTAAPARIDAQADAVLAWTDTASVAVQIVEPRATASRRAP"
BASE COUNT          464 a          963 c          926 g          338 t
ORIGIN      
        1 cccgagtacc ccgagtggct cgcgcgatcg ggcgacccga tcgacttctc gaccgacgac
       61 agcgagatga cgctggcgat cgagtggagc gaggaggtca tcgagcagct gccggaggac
      121 ctgacgatcg agctctccgg cccgcgcctg gcgacccgca gcttcgcgct ctcgctcgcc
      181 agccgcggcg gtgggatcgc gcgactgacc ttcggcgagc tgcagcgcag ccaggcgatc
      241 acgctgaccg ccaagcacgg cgacgcggag ctgctgctgg tgcgcgacca gatcgccggc
      301 gacctcgagc aggcgatggt gtgggagcac cacctggagg agctgctggt gcccgccgag
      361 ccggaggaag acggcgagct gatcgcgtcc ggcgcaaccc ccgatgacgc gcacggagag
      421 gtcgcggtgg cctgatatgg atcacgagtg gatcgtcgag gtcaaggacg acaagggcgc
      481 ggcgctgacg aacgccacgg tcgcgctcgt cgagacgagc gcgctcgccg gtcaggactg
      541 gccgtacacg gccgccaagg cgacgcacgc ccacgacgcc ggcggcacct acaaggccgc
      601 accgccgatc gtgccggtcg ccggcgagtg gatgttgatc gtccgcgtcg ccggcaagtc
      661 gccggtcgtg cagccgctca agatggtcgt caccaagacc gacgtgatca ccacgccctc
      721 gccaaggacc gcggcgacgg tgaccttcag ctcggaggtc aagacggtcg cgaccagcaa
      781 gacccggcgc gcgcgcctcg gcgtcaagat gttcccgtcc tcggagctgg tgttcctcag
      841 cggcaccgag tacttcaacg ccggcacgcg ctttcgcatc ttcgccgaga actaccgcga
      901 gggcctgcgc cgcgagaagc tcgtcgacgc cggcgtgatc gccacgctgc tgtcgaccga
      961 cgaccgctcg cgcgagaccc acgtaccggc gatggccggc ggctggctca aggtcggtca
     1021 caagagcttc ggtcccacct cggggatcaa gcccggcacc gagcacgcgc cggtggtcgg
     1081 tcaggacgtg tcgatcgtcg acctctacaa gtacctcagc caggtcggcg cgagcgagcc
     1141 cgggcgcgtc aaggaggtcg gcatcttctc gcactcgtgg ccgggcggcc cgctgctgtt
     1201 caacaccagc gacaacagcc tcacctccgc gcgcgacatc gacgacttcg acggccggcg
     1261 caaggacttc aacgcgacca acgtcgccgg ctggccgaag atgaaggcgg cgatggcccc
     1321 gggcggcacc tggcacgtgt ggggttgcag cgcgacgacg catcacaaga acctgaccgt
     1381 cgccgcgcac aagcacaaaa aggacggcga caccgcgttc ttcacggtga cgacgagcgt
     1441 gcagcaccac ggcggcgcca cggcgagcac gatggaggag cgtaccaacc gcgcgaggat
     1501 ccgcttcgac atggatcagc ggttccgttc gctgagctac atggccgccg ccgtcagcca
     1561 cctggcgatc cccgtgttcg gcgcgccgcc gggggtcgga tccagcttcg accagaagcg
     1621 cagcatcatg ttcatcgaca ccgcgtcgta cgccaacctg ttcgcgtact tcaaggacgt
     1681 gttcggcccc gagttcacgc cgacgagcag cacctacgac cgcggctacg tcgactatca
     1741 aaaggtcggc ggccggccgg ccccgccggc ccccggcttc tcgtccgagg cctacatttt
     1801 taataaggac ttcgataata aaaagacgac gctgtccgtc gacagcaaaa agacggtgac
     1861 gatggccggt acggcgatca cgctcaaggt gatcccaaag accggcttcg ccacgccggg
     1921 caagtcgggt catcagttcg agttgcacga caccgccgac aagacccgct cgcgcgcgat
     1981 ctacgtgcag gacgacaagc agaccttcaa cgtcaagctc gacggcgccg gcaagttcac
     2041 ggtgctcggc gcgacgctgt agcgcgtggc tcgacgatct gcacggcgac cgacgcggtg
     2101 tccgtccagg cgagcacggc gtcggcctgc gcgtcgatgc gggccggcgc ggccgtcaac
     2161 acgagcacga gcctgcgcgc cgcgggatcc cacgggaggc cctgcgcggc cgccagcgcg
     2221 gcgaatatgg gcctcgggcg gtcgtcgggc gacccggcgc gcagcctgcg aagcgcgccc
     2281 ggcacgacgc ccggcgcctg tgtgagcccg gcgacgacgc tcgcgccgtc gccgtcccag
     2341 cgcagcagcg cgacgcgatg gcccgcggtc gcgacgaagc ggtcgagcgc cggctgcaat
     2401 gcaccgaggc ccgcgagcga ctggcccatt ttaccacggc cgtcgaccac gagggcgacg
     2461 tcggccggcg cgtcggcgag gccggcgagc gcgtcggaga cggcgaccct cagcgcctcc
     2521 gacgagtcgg cgcgcacgtg ccgaccgtcg cgcacgagcc cggcgatcac cgcgagcggc
     2581 gcccctggat ccggcggcgc ctcgggcccc tcgccgcggc catcgcggag ctcgaggcgg
     2641 cacagcgcga tgtgcacgac ggccccacgc gcccacgctg cgacggcgcc g
//
LOCUS       DASWNJ010001853         2233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_526754, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001853 DASWNJ010000000
VERSION     DASWNJ010001853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526754"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="VGB85_30940"
     CDS             <1..986
                     /locus_tag="VGB85_30940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138555.1"
                     /translation="LERLRRRGDPARLLELLPRIKRNEYLAPVLQILLSRTPLPIEAA
                     VPQLGSVHPRVAAVAAEIVGRAGPAAAKAHGKRLAEVLRGAREAWLAESATLGDGKSH
                     RLDEVEPAYRSALWAAGRLDVGADEIVQALTLAEGTAFPARGRALRRQAALSLADGAG
                     GKAGLEALRAVLKDSHDAELRSIAAGGLAQLDTGKGGIAKLVPAVLDDGPSLARLVGP
                     EALPALRGSAGDPHRQGTVLRHLVAQGDSAGLIKALAETKHETARLGLVEALGKVADE
                     PAKAALRRLGADEAEDEELRKAAWRALRRAKRISREATSPRARHGRWEVQP"
     gene            983..>2233
                     /locus_tag="VGB85_30945"
     CDS             983..>2233
                     /locus_tag="VGB85_30945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138556.1"
                     /translation="MSEETDKPEGGPEQESTRPEEQVSDPRTPITLTYAGASQLVTPS
                     SGAEGAGTDTRLALFTNTRRADVRAELTAKDPLTLREALSCLYEVVSSDFRYVPRDKT
                     AYLAYMRLRKSMAGQGAVQAQQAYFEWLSRNDPTAWLVLDPIVTVHPDRLIFEVFSKD
                     EGAYAQLGVDWSAFEVAGTTRHGTTNIDFSKGLFDGVQRMRSYRPTRLSIGREAVSVH
                     TEGKPPVIEKTIQVPDAWLRGFLQVQSAATLPTTVVTIGAIDLYNLLRQLRLHADLKK
                     GGRGVRIELVPGEAPRLVIEPWEIVLTSAAGPYKGRTPALIRIWGRRRLSLLRRMLPF
                     VETVDIHLLGSGLPSFYVLRAGPITLTLGLSGFTSANWSQSVGFDQLLPRDRHDDIKT
                     THAAVLKQLGEVWVASAEELAKATK"
BASE COUNT          336 a          780 c          833 g          284 t
ORIGIN      
        1 cgctcgagcg cctgcgcagg cgcggagacc cggcacggtt gctcgagctg ctgccgagga
       61 tcaagcgcaa cgagtacctc gcgccggtcc tgcagatctt gctgtcgcgc acgccgttgc
      121 cgatcgaagc cgcggtgccg cagctcggct cggtgcaccc gcgcgtcgcc gcggtcgcgg
      181 cggagatcgt cgggcgcgcc ggaccggcgg cggccaaggc ccacggcaag cgcctcgcgg
      241 aggtgctccg cggcgcgcgt gaggcctggc tcgccgagtc cgccacgctc ggcgacggca
      301 agtcgcatcg cctcgacgag gtcgagccgg cctaccgcag cgcgctgtgg gccgcgggtc
      361 gcctcgatgt cggcgccgac gagatcgtgc aggcgctgac gctcgcggag ggcacggcgt
      421 tcccggcccg cgggcgggcg ctgcgtcgcc aggccgcgct gagcctcgcg gacggggccg
      481 gcggcaaggc cgggctcgag gcgctgcgag ctgtcctcaa ggacagccac gacgccgagc
      541 tgcgcagcat cgccgctggc ggcctcgcgc agctcgacac cggcaagggt gggatcgcca
      601 agctggtgcc cgcggtgctc gacgacggcc cgagcctcgc gcggctggtc ggccccgagg
      661 cgctgccggc cctgcgcggc tcggccggcg acccgcaccg ccagggcacg gtgctgcgcc
      721 acctggtcgc ccagggcgac agcgccgggc tgatcaaggc gctcgcggag acgaagcacg
      781 agacggcgcg cctcggcctg gtcgaggcgc tcggcaaggt cgccgacgag ccggcgaagg
      841 cggcgctgcg gaggctcggc gccgacgagg ccgaggacga ggagctgcgc aaggccgcct
      901 ggcgggccct gcggcgcgcc aaacggatct ccagagaagc aaccagcccg cgtgcgcggc
      961 acggccggtg ggaggtgcaa ccgtgagcga agagaccgac aagcccgaag gcgggcccga
     1021 gcaagagtcg accaggcccg aggagcaggt cagcgacccc cgcacaccga tcacgctcac
     1081 ctacgcgggc gcgagccagc tggtgacgcc gtcgtcgggg gccgagggcg ccggcacgga
     1141 caccaggctg gcgctgttca ccaacacgcg ccgcgccgac gtgcgcgccg agctgaccgc
     1201 gaaggaccca ctgacgctgc gcgaggcgct gtcgtgcctg tacgaggtcg tcagctcgga
     1261 cttccgctac gtaccgcgcg acaagaccgc gtacctcgcg tacatgcggc tgcgcaagtc
     1321 gatggccggt cagggggccg tgcaggcgca gcaggcctac ttcgagtggc tgtcgcgcaa
     1381 cgacccgacc gcgtggctgg tgctcgaccc gatcgtgacg gtgcacccgg accgcctgat
     1441 cttcgaggtg ttttcaaaag acgagggcgc gtacgcgcag ctcggcgtcg actggtcggc
     1501 cttcgaggtc gccggcacga cgcggcacgg cacgaccaac atcgacttca gtaaaggcct
     1561 gttcgacggc gtgcagcgga tgcgcagcta tcggccgacg cgcctgtcga tcggccgcga
     1621 ggcggtcagc gtgcacaccg agggcaagcc gccggtgatc gagaagacga tccaggtgcc
     1681 cgacgcgtgg ctgcgtggat ttttacaggt gcagtcggcg gcgacgctgc cgaccacggt
     1741 cgtcacgatc ggcgcgatcg atctttataa tttgctgagg cagctgcgcc tgcacgccga
     1801 tctgaagaag ggcggccgcg gcgttcgcat tgaattggtc cccggcgagg cgccgcgcct
     1861 cgtgatcgag ccgtgggaga tcgtgctgac gtccgcggcc gggccgtaca agggccgcac
     1921 gccggcgttg atccgcatct ggggccggcg gcgcctgagt ctgctccggc gcatgctgcc
     1981 ctttgtcgag acggtcgaca tccacctgct cggcagcggg ttgccgagct tctacgtgct
     2041 gcgcgccggc ccgatcacgc tgacgctcgg gctgtcgggc ttcaccagcg cgaactggtc
     2101 gcagtcggtc ggcttcgacc agctgctacc gagagatcgc cacgacgaca tcaagaccac
     2161 ccacgccgcg gtgctcaagc agctcggcga ggtctgggtc gccagcgccg aggagctggc
     2221 gaaggcgacg aag
//
LOCUS       DASWNJ010001854         1961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_104223, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001854 DASWNJ010000000
VERSION     DASWNJ010001854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104223"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..1069)
                     /locus_tag="VGB85_30950"
     CDS             complement(224..1069)
                     /locus_tag="VGB85_30950"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEY0138557.1"
                     /translation="MTSTSTTTAPKELGEIRRRDMDFAFSEDFPRYWAAGAAVPTHMA
                     NALHLIFPMGERFFVRAVRRYEASITDPLLRAQIKGFYAQEGSHAREHERVFAMIEGQ
                     GYDIQGFLSLYRKLAYSFIERVSPAKLRLAATAALEHYTAIMAENALLHGLVDRIHPE
                     MRALLLWHACEEIEHKAVAYDVLQAVAPGYALRVAGMVMATSTLIGFWFLGTAMLLRQ
                     EKDLGWREIVRQLRASGQENPVGRKVFVRGLREYLRRDFHPWQTDNLGLARAYLARVT
                     GAAAA"
     gene            1145..1759
                     /locus_tag="VGB85_30955"
     CDS             1145..1759
                     /locus_tag="VGB85_30955"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0138558.1"
                     /translation="MAAPQRNARERARLGVDERRSQLLELGLQLFADHSYDELGIDDI
                     ARAAGISKGLLYHYFPSKRDYYVEVVRSAAALLLGRTAEAAGTVSPEALHHGLDAYLD
                     FVEQHARSYTALVRGGIGSDPEVVQLLEATRRTIAERILSKLSSDPPSALLRAALRGW
                     IGMVEAASLDWLERRDLPRPQLRELLAQMLLAALAAAGAVPPGL"
BASE COUNT          294 a          717 c          657 g          293 t
ORIGIN      
        1 ccgtgggcga ccagctcggc gtgctggcgc gacaccgagt cgctcgcgag cacgacgtcg
       61 cagtccggcg agcggccgat caccacgcgt gtccccgtca ggtggctgtt ggccaccagg
      121 gagttctcct tgcggatctg caaccagccg cccatggtcc cggccaccat acaacaagaa
      181 cacgacgcgc gacgctccgg cgccggcagt gcgcgctcgc gagctaggcc gccgcggccc
      241 cggtcacgcg cgccaggtag gcgcgcgcca gcccgaggtt gtcggtctgc cacgggtgga
      301 agtcgcggcg caggtactcg cgcaggccgc ggacgaacac cttgcggccg accgggttct
      361 cctggcccga ggcccgcagc tgccgcacga tctcacgcca cccgaggtcc ttctcctggc
      421 gcagcagcat cgccgtgccg aggaaccaga acccgatcag cgtcgaggtc gccatcacca
      481 tgccggcgac ccgcagcgcg tagccgggcg cgaccgcctg caacacgtcg tacgcgaccg
      541 ccttgtgctc gatctcctcg caggcgtgcc acagcagcag cgcccgcatc tccgggtgga
      601 tccggtcgac caggccgtgc aggagcgcat tctcggccat gatcgccgtg tagtgctcga
      661 gcgccgccgt cgccgcgagc cgcagcttcg ccgggctcac ccgctcgatg aacgagtacg
      721 cgagcttgcg gtacagcgac aggaacccct ggatgtcgta gccctgcccc tcgatcatcg
      781 cgaacacccg ctcgtgctcg cgcgcgtggc tgccctcctg ggcgtaaaag cccttgatct
      841 gggcccgcag caggggatcc gtgatgctcg cctcatagcg ccggacggcg cgcacgaaga
      901 agcgctcgcc catcgggaag atcaggtgca gcgcgttcgc catgtgcgtc ggcaccgccg
      961 cgcccgccgc ccagtacctc ggaaaatctt cggagaacgc gaagtccatg tcccgccggc
     1021 ggatctcccc gagctccttc ggcgccgtcg tcgtgctcgt gctcgtcatc gcagggccct
     1081 cctgccgcca gactacccgc tattgaacgg aggtcaatag tcgatcgggt atcttgtgcg
     1141 ccccgtggcc gccccgcaac gcaacgcgcg agaacgcgcc cgcctcgggg tcgacgagcg
     1201 ccggagccag ctgctcgagc tgggcctcca gctgttcgcc gaccacagct acgacgagct
     1261 cggcatcgac gacatcgcgc gcgcggcggg catctcgaag gggctgctct atcactactt
     1321 cccgagcaag cgcgattact acgtcgaggt ggtgcgcagc gctgcagccc tgttgctcgg
     1381 gcgcaccgcc gaggccgcgg gcacggtctc acccgaggcg ctgcaccacg gcctcgacgc
     1441 ctacctcgac ttcgtcgagc agcacgcccg aagttacacg gcgctggtcc gcggcggcat
     1501 cggcagcgac cccgaggtcg tgcagctgct cgaggccacg cgccgcacga tcgccgagcg
     1561 catcctctcg aagctcagca gcgacccgcc ttctgccctg ctgcgtgccg cgctgcgcgg
     1621 gtggatcggg atggtcgagg cggcgagcct cgactggctc gagcgacgag atcttccgcg
     1681 cccgcagctg cgggagctgc tcgcgcagat gctgctcgcg gcgctcgctg ctgcgggggc
     1741 cgtgccgccg gggctgtagc gccttagtac ctggtcgcgc gagcgtcgtt gggctggcgc
     1801 ggacctaggc tgtcttgctc ctagtactac ttgatcagat ggtcgagggc ccgctcggcg
     1861 gcgagcgggg tgtaacacga cgccggcaga gcgggcaggt cccgatcatg ctgatgagga
     1921 cctgctgaag gactcgacgg accatcggca gggtccagct c
//
LOCUS       DASWNJ010001855         1702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_629215, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001855 DASWNJ010000000
VERSION     DASWNJ010001855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629215"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(270..731)
                     /locus_tag="VGB85_30960"
     CDS             complement(270..731)
                     /locus_tag="VGB85_30960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138559.1"
                     /translation="MRSRPELLLVLWLAGCGDTGDGSGDGSSGAAGDETSTSSGSGET
                     PTTGGEPASLEERACPEDSALSSENFGAPFMLTHCTGCHHSSLPASERAGAPLAIDFE
                     TIDLVRTHAPRIWARAADHNATMPPLGGPPLEERTRLGEWLACGAPTASEL"
     gene            complement(715..>1702)
                     /locus_tag="VGB85_30965"
     CDS             complement(715..>1702)
                     /locus_tag="VGB85_30965"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY0138560.1"
                     /translation="FTAHAGSCAIVRGLQVSSVAHPDASHRVLTGDSADTAPDVAAIA
                     SAVHGADMAAPYLILGRTAYSGPYGSLAARTGSVNQIVTLLDPKIALPPIGGPLLPHV
                     PSDAEEALVRAHVLARAERELTASGGNTRVDDFIASLGRGDLLRDIGIVGELELARSF
                     TAQIDLAVEGLARGLCHSVQIETGDWDTHTDNAKQGPLHDQFFAGLGQLIEALATRAG
                     STQGSKLLDETTVVVVSELGRTPRLNSEQGKDHWPVTSALVIGSGVAGGRTIGATTDG
                     LDGASVDLTTGKPDANGKPLLYSNFAAGLLELAGIDPGEYIPGAEALHALSA"
BASE COUNT          227 a          619 c          632 g          224 t
ORIGIN      
        1 aaggccacgg cagtgagcac ggcgcccccg ccggccacgg ccccgccgtc aaggcagccc
       61 agggccagcc cgacaccaag cagcccgagg gcgcccacaa caacggcgcc gacggcacgc
      121 gcaagtcggc ccccgccggc gaggatgtcg cggcgcactg agccctgact cgctcgaaca
      181 agacggccgc cggggtgacc tggtggccga ttttgctcgt cgcgcccgcg cgagacgagc
      241 tgcgagcgct catgccatcg gcacggcgct cacagctcgg aggccgtcgg cgccccgcaa
      301 gcgagccact cgccgaggcg cgtgcgctcc tccagcggcg gcccgccgag cggcggcatc
      361 gtggcgttat ggtccgcggc gcgggcccag atccgcgggg cgtgcgtgcg gaccaggtcg
      421 atcgtctcga agtcgatcgc cagcggcgcg ccggcccgct cgctcgccgg caggctcgag
      481 tgatggcagc cggtgcagtg cgtcagcatg aagggcgcgc cgaagttctc cgagctgagc
      541 gcgctgtcct ccgggcacgc gcgctcctcg aggctcgccg gctcaccccc ggtcgtcggc
      601 gtctcgccac tgccactgct ggtgctcgtc tcgtcgccgg ccgcgccact gcttccgtcg
      661 ccgcttccgt cgccggtatc gccgcagccc gcgagccaca gcacgagcaa gagctcaggc
      721 cgagagcgca tgcagggcct ccgcgccggg gatgtactcg cccgggtcga tgccggcgag
      781 ctcgagcagg ccggcggcga agttggaata gagcagcggc ttgccgtttg cgtcgggctt
      841 gccggtcgtc aggtcgaccg acgcgccgtc gaggccatcg gtggtcgcgc cgatcgtgcg
      901 gccacccgcg acgccgctgc cgatcaccag cgcgctggtc accggccagt gatccttgcc
      961 ctgctcgctg ttgagccgcg gcgtgcggcc gagctcggag acgacgacca ccgtggtctc
     1021 gtcgagcagc ttcgatccct gggtgctgcc ggcccgggtc gccagcgcct cgatcaactg
     1081 gccgaggccg gcgaagaact ggtcatgcag cgggccctgt ttggcgttgt cggtgtgcgt
     1141 gtcccagtcg ccggtctcga tctgcaccga gtgacacagg ccgcgcgcca gcccctcgac
     1201 cgcgaggtcg atctgcgcgg tgaacgagcg ggccagctcg agctcgccga cgatgccgat
     1261 gtcgcgcagc aggtcaccgc gcccgagcga ggcgatgaag tcgtcgacgc gggtattgcc
     1321 accggaggcc gtcagctcgc gctcggcgcg ggcgagcacg tgggcgcgca ccagggcctc
     1381 ctccgcgtcg ctcggcacgt gcggcagcag cgggccaccg atcggcggca gggcgatctt
     1441 cgggtcgagc agcgtgacga tctggttgac ggagccggtg cgcgccgcga gcgacccgta
     1501 cgggcccgag taggcggtgc ggccgaggat caggtacggc gcggccatgt cggcgccgtg
     1561 caccgcggag gcgatcgccg cgacgtcggg ggccgtatcg gcgctgtccc cggtgagcac
     1621 gcgatgcgag gcgtcggggt gcgccaccga gctgacctgc aggccgcgga cgatcgcgca
     1681 gctgcccgcg tgagccgtga ag
//
LOCUS       DASWNJ010001856         2873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_104269, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001856 DASWNJ010000000
VERSION     DASWNJ010001856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104269"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1288)
                     /locus_tag="VGB85_30970"
     CDS             complement(74..1288)
                     /locus_tag="VGB85_30970"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0138561.1"
                     /translation="MRGLDLVNVFMADVKDGVGVYLAVYLLAEQHWDPAHIGIVVAIP
                     WLVAIAVQAPVGGFIDRTPHKRLLLIVASAVVALSCLTVVFFPDLTPIAAAQVALGVA
                     QTIFPPCVAAITLGIVGHERLSGRIGRNESFNHGGNMLSAVVAIFIGAYISYRGIFYF
                     SVFQCAAIIAATLLIRERDIDHELARAAAAESTHAPLAGVKALLADGDIARFIAAMCL
                     WNVANGAMLPLLGQKLGVADVEHSALSLSICIIIAQAVMIAVAPVTAKLADGGRKRLF
                     LVAFLLVPVRAVLFALVDNKVALVTFQLIDGLGAGIYGVLLIVMMADLGKGTGRFNLL
                     QGTTYAAVGLGVALSSILGGLMVRQAGFPAAFAVLAAVGVLATSFFAACVREPPRRRP
                     WAGAPEIARCGD"
     gene            1546..>2873
                     /locus_tag="VGB85_30975"
     CDS             1546..>2873
                     /locus_tag="VGB85_30975"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEY0138562.1"
                     /translation="MGEHLELWGGVECTKNRVGDQFFDQLDRNGHEERASDLERFAAL
                     GVRALRHPVLWGRVCPERPDVPDWRRADACLGRIRELGITPIVGLVHHGSGPRYTDLL
                     DPQFPEKLAAYARTVAERYPWVRMYTPVNEPLTTARFSALYGHWYPHARDEAKFARAL
                     LNQVRGVALAMEEIRRVNPEARLVQTDDLGMTHATPQLRADAEFQNERRWLTWDLLCG
                     RFAPDHVMRRHFRAHGVPEGELQWFVEHPCPPDIIGINHYVTSERFLDHRMDRYPRCT
                     YGGVDRDRYADIEAVRIRTAGLVGPEALLMETWQRYALPIAVTECHLGCTREEQLRWL
                     LAMWNAAQSAKDRGADIRAVTVWSLLGAYDWCSLVCKDCGSYEPGPFDLRAPQPRPTA
                     LATMMRQLGSGETPEHPVLQAPGWWQRPIRLLYPEHDAAAQAPRAADEPTP"
BASE COUNT          476 a          997 c         1009 g          391 t
ORIGIN      
        1 ccccggctga tcggggccct cgtgcgcgcg ggcggtggat cgctccgtgt acctcaaggc
       61 acgacgccga tggtcaatcg ccgcacctcg caatctccgg cgcgccagcc cacgggcgtc
      121 gccgcggcgg ctcgcgcacg caagcggcga agaacgacgt cgccagcacg ccgaccgcgg
      181 ccagcacggc gaaggccgcc gggaacccgg cctgcctgac catcaagccg ccgaggatgc
      241 tgctgagggc cacgccgagg cccacggccg cgtaggtcgt gccctgcagc agattgaagc
      301 gcccggtccc cttgccgagg tcggccatca tcacgatcag caggactccg tagataccgg
      361 cgccgagacc gtcgatcagc tggaacgtga cgagcgcgac cttgttgtcg accagcgcga
      421 acaggaccgc ccgcaccggg accagcagga aagcgacgag gaacaggcgc tttcggccgc
      481 cgtcggccag cttcgccgta accggcgcca cggcgatcat gacggcctgg gcgatgatga
      541 tgcagatcga cagggacagc gccgagtgct cgacgtccgc gacgcccaac ttttgcccga
      601 gcagcgggag catcgcgccg ttggcgacgt tccacaggca catcgcggcg atgaagcggg
      661 cgatgtcgcc gtcggcgagc agcgccttca cgccggccag cggggcgtgc gtcgactcgg
      721 ccgcggcggc gcgggccagc tcgtggtcga tgtcccgctc gcggatcagc agggtcgcgg
      781 cgatgatcgc cgcgcactga aacacggaga aatagaagat gccgcgatac gagatgtacg
      841 cgccgatgaa gatcgccacc acggcggaca gcatgttgcc gccgtgattg aagctctcgt
      901 tgcggccgat gcgtccggac aggcgctcgt ggccgacgat ccccagggtg atggcggcca
      961 cacacggcgg gaagatcgtc tgcgcgaccc cgagggccac ctgcgccgcg gcgatcgggg
     1021 tcaggtcggg gaagaacacg accgtgaggc aggagagcgc gacgacggcc gacgcgacga
     1081 tcaacagcag ccgcttgtgc ggggtgcggt cgatgaaacc gccgacgggc gcctggacgg
     1141 cgatcgccac cagccacgga attgcgacca cgatcccgat gtgggcggga tcccagtgct
     1201 gctcggcgag caggtagacg gccagataca cgcccacccc gtccttgacg tccgccatga
     1261 agacgttgac gaggtcgaga cctcgcaagc tgcggcttga agcgtgcaat ccggccgcct
     1321 ttcgccgaca acatgccgcg tccccgggtg ctcgccagtg cggcggccgc agcggcgtgc
     1381 ggcgcgtgcg gcgcggcctc gcggtcgacg aagttcttgc gcatgcacga tgctggcaca
     1441 tgacttcggg gcgagccgac tctggacccg cgtatcaacc cggatcttca cggggcggtg
     1501 ggcccgagcc ataaatcgga gactgtcatg aatgatattc atgcattggg agagcatctg
     1561 gagctgtggg gtggcgtcga gtgcacgaag aaccgcgtcg gtgaccagtt cttcgatcag
     1621 ctggaccgca acgggcacga ggagcgcgcg tcggacctcg agcgcttcgc ggccctgggg
     1681 gtgcgggcgc tgcgtcatcc ggtgctgtgg gggcgagtct gcccggagcg cccggacgtg
     1741 ccggactgga ggcgcgcgga cgcctgtctc ggcaggatcc gcgagctggg gatcacaccg
     1801 atcgtcgggc tggtgcatca cggcagcgga ccgcgctaca cggacctgct cgacccgcag
     1861 ttccccgaga agctggccgc gtacgcccgg acggtggccg agcgctatcc gtgggttcgc
     1921 atgtacacgc cggtcaacga gccgctgacc acggcccgct tcagcgcgct ctacggccac
     1981 tggtacccgc acgcccgcga cgaggcgaag ttcgcccgcg cgctgctcaa ccaggtccgt
     2041 ggcgtcgcgc tcgccatgga ggagatccgt cgggtcaatc ccgaggcccg gctggtccag
     2101 acggatgacc tcggcatgac ccacgcgacc ccgcagttgc gagccgacgc cgagtttcag
     2161 aacgagcgcc gctggctgac ctgggacctg ctgtgcggcc gcttcgcgcc ggaccacgtg
     2221 atgcgccgcc acttccgggc tcatggtgtt cccgagggcg agctgcagtg gttcgtcgag
     2281 cacccgtgcc cgcccgacat catcggcatc aatcactacg tgacgagcga gcgcttcctc
     2341 gaccaccgaa tggaccgcta tccgcgatgc acgtacggcg gcgtcgaccg cgaccgctac
     2401 gccgatatcg aggccgtacg gatccgcacc gccgggctcg tcggccccga ggcgctgctc
     2461 atggagacct ggcagcgcta tgccctcccg atcgccgtga ccgagtgcca cctcggctgc
     2521 acccgcgagg agcagctgcg ctggctgctc gccatgtgga acgcggcgca gtcggcgaag
     2581 gaccgcggcg cagacatccg cgccgtgacc gtgtggtcac tgctgggtgc gtacgactgg
     2641 tgcagcctgg tttgtaagga ctgcggcagc tacgagcccg gccccttcga cctgcgcgcc
     2701 ccccaaccgc gcccgaccgc gctggcgacg atgatgcggc aactcggcag cggcgagacc
     2761 ccggagcacc cggtgttgca ggcgccgggc tggtggcagc ggccgatacg gctgctgtac
     2821 cccgagcacg acgcggcagc ccaggcgccg cgggccgcgg acgagcccac ccc
//
LOCUS       DASWNJ010001857         2274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_669089, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001857 DASWNJ010000000
VERSION     DASWNJ010001857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669089"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VGB85_30980"
     CDS             <1..358
                     /locus_tag="VGB85_30980"
                     /inference="COORDINATES: protein motif:HMM:NF024894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HEY0138563.1"
                     /translation="LADVAPQLVALAGGSRLADDLVREGHGRRWGVFLTSRLSLVDLR
                     RHLRRFLLARLVGGEDRVFFRYYDPGVLQVYLASCNAEERAAWFGGAGEHIDCFMGED
                     EAAGDGWWRATAAGVE"
     gene            complement(363..1115)
                     /locus_tag="VGB85_30985"
     CDS             complement(363..1115)
                     /locus_tag="VGB85_30985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138564.1"
                     /translation="MIDYLNITTRGVASEILVNGAPILRTPIDAPCAAIPSITEWLIA
                     GDNRITAAVYALSADEPPQLRVQLCAGRLGEFVTPGEELARGELALHLDPDAPPTLPA
                     AIDLHESRVGGPRWAWQDAARWTLDEDELAELWLYIELLHGYLQDGHIDDLVAHMRIK
                     YAELGPLYGAAPDAAAAVLRQHFVELSAGGAWRVAPLVRADLLLRPVCDARLVTPVDR
                     DGEPALRGLGSSGPWELPVFVGRVAGGLEILR"
     gene            complement(1112..>2274)
                     /locus_tag="VGB85_30990"
     CDS             complement(1112..>2274)
                     /locus_tag="VGB85_30990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138565.1"
                     /translation="VDRVIAATRSQGVFAGLRRLLAPPLSDYRLDVLTCGARPQPHAF
                     KRFEYGLVVYPPDTFKIAISLPSWKKIERKSSTSISRDGRRDESTRSTAYGLSGRETS
                     RSESTYDSQRGYVVRQVDTQADRSGGTRRTQTLAESRAGSFVHRDRSGRADATDGLVK
                     STGKTLTISHDNKDISSSLKVVEFIEFVADLRANVMKFWDFLQSLTKKAPKIGWSLSL
                     ELEFLSGSFEYEWGYREWKDHSVYYYWKAEAALTIATIRAEIAFGLTVLGVNIQVFGA
                     FTAELKVSGAKEADPDRTGPALTFSQTVNPGLEVGVRGALGDWVKVVGKIAGNLDIAN
                     KMELGPFKWTIKIELAEGKGTLTLTSKFGFNRTASTSLWDKYVILPEKTLIG"
BASE COUNT          313 a          771 c          830 g          360 t
ORIGIN      
        1 gctcgccgac gtcgcgccgc agctcgtcgc gctcgcgggc ggctcgcggc tcgccgacga
       61 cctcgtgcgc gagggtcatg gccggcgctg gggcgtgttc ctgacgtcgc gcctgtcgct
      121 ggtcgatctg cgccgtcacc tgcgtcgctt cctgctcgcg cggctggtcg gtggcgagga
      181 ccgggtgttc tttcgctact acgacccggg cgtgctgcag gtgtacctgg ccagctgcaa
      241 cgccgaggag cgcgcggcgt ggttcggcgg cgcgggcgag cacatcgact gcttcatggg
      301 cgaggacgag gcggccggcg acggctggtg gcgagcgacc gcggccgggg tggagtaact
      361 cctcagcgca agatctcgag ccccccggcg acgcgaccga cgaacaccgg cagctcccac
      421 ggcccgctgc tgccgagccc gcgcagggca ggctcgccgt cgcggtcgac cggcgtcacg
      481 agacgagcgt cgcagaccgg ccgcagcagc aggtcggcgc ggacgagcgg cgcgacccgc
      541 caggccccgc cggcggacag ctcgacgaag tgctggcgca gcaccgccgc ggccgcgtcg
      601 ggcgcggccc cgtagagcgg cccgagctcg gcgtacttga tgcgcatgtg cgcgacgagg
      661 tcgtcgatgt gaccgtcttg caggtagccg tgcagcagct cgatgtacag ccacagctcg
      721 gcgagctcgt cctcgtcgag ggtccagcgg gcggcgtcct gccaggccca gcgcggcccg
      781 ccgacccgcg actcgtggag gtcgatcgcg gcgggcaggg tcggcggcgc gtcggggtcg
      841 aggtgcagcg ccagctcgcc gcgcgccagc tcctcgcccg gcgtgacgaa ctcgccgagc
      901 cgcccggcgc acagctgcac gcgcagctgc ggcggctcgt cggcggagag cgcgtacacg
      961 gcggcggtga tccggttgtc gccggcgatc agccactccg taatcgatgg gatcgccgcg
     1021 cacggcgcgt cgatcggcgt gcgcaggatc ggggcgccgt tgacgaggat ctccgacgcg
     1081 acgccgcggg tcgtgatgtt gagatagtcg atcatccgat cagtgtcttt tcggggagga
     1141 tgacgtactt gtcccacagc gaggtggagg ccgtgcggtt gaagccgaac ttgctcgtca
     1201 gcgtcagcgt gcccttgccc tccgccagct cgatcttgat ggtccacttg aacggcccga
     1261 gctccatctt gttggcgatg tcgaggttgc cggcgatctt gccgaccacc ttcacccagt
     1321 cgcccagagc gccgcgcaca ccgacctcca ggcccggatt gacggtctgg gagaaggtca
     1381 gcgccgggcc ggtgcggtcg gggtcggcct cctttgcgcc gctgaccttg agctcggcgg
     1441 tgaaggctcc gaagacctgg atgttgacgc cgagcaccgt caggccgaac gcgatctcgg
     1501 cgcgaatggt cgcgatcgtc agcgccgcct cggccttcca gtaatagtag acgctgtggt
     1561 ccttccactc gcggtagccc cactcgtact cgaagctgcc ggacaggaac tcgagctcga
     1621 ggctgaggct ccacccgatc ttcggggcct tcttcgtcag ggactgcagg aagtcccaga
     1681 acttcatcac gttcgcgcgc aggtcggcga cgaactcgat gaactcgacg accttgagcg
     1741 acgacgagat gtccttgttg tcgtgcgaga tcgtcagcgt cttgccggtc gacttgacga
     1801 gcccgtcagt cgcgtcggcc ctgccggagc ggtcccggtg gacaaagctg ccggcgcgcg
     1861 actcggcgag cgtctgggtg cgccgggtgc cgccgctgcg gtccgcctgg gtgtcgacct
     1921 ggcggaccac gtagccgcgc tgcgagtcgt aggtgctctc gctgcgcgat gtctcgcggc
     1981 cgctgaggcc gtacgcggtc gagcgcgtgc tctcgtcacg ccgcccgtcg cgggagatcg
     2041 aggtcgagga ctttcgctcg atcttcttcc agctcggcag cgagatcgcg atcttgaagg
     2101 tgtcgggcgg gtagaccacg aggccgtatt cgaagcgctt gaacgcgtgc ggctgcggtc
     2161 gcgcgccgca ggtcaggacg tcgaggcgat agtccgacag cggcggggcg agcagccggc
     2221 gcagcccggc gaacacgcct tgcgagcgcg tcgcggcgat aacccggtcg accc
//
LOCUS       DASWNJ010001858         7768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_526890, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001858 DASWNJ010000000
VERSION     DASWNJ010001858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7768
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526890"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VGB85_30995"
     CDS             <1..398
                     /locus_tag="VGB85_30995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138566.1"
                     /translation="LAVGLLGLAGAPPTGAFLARVALLQAGFQDSNLLVRVALGAGAL
                     AGVALALACLRVLATMCSATGDEPPRGRAWARGTLLALAAMLLVTGLFGQVTVEAARA
                     AAAGAGLSPGSPARRAWIESRQRGPDAPR"
     gene            786..3362
                     /locus_tag="VGB85_31000"
     CDS             786..3362
                     /locus_tag="VGB85_31000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138567.1"
                     /translation="MKTYLARYWAVYGISLVAPGLAACGDSTMATTEGTTESGTTEVT
                     PTSSTGLTTTGDDSTSTTDTPTTLGSNSESESGSSSTTEATTTTGDTTTDDTTGQVTA
                     TTGGTSTGGPDVCMTILCGDPAVCCLETDECVGGTCQPICPSGVRCGAEQEMCCEEGD
                     VCVGDTCTTPLGDCNDSYDCSPGNYCEPVLKKCLPQPDPLQCEVVPTFDKIDVQLEWS
                     WTVNEVTVTPMVADVQGDETPDVVVNAFRTDGIDRNLGEMVLLDGKTGTEIWRLKHDP
                     VNGTFGSFGLGTPALGDLNGDQKPDIVYPGRWTSNPPPAPNANPNSEIGPVHAVDGTG
                     KLLWTGHTANNPAVNIRWAHAAAAMANLDDDPEAEIAVGGAIFDNDGLLVWNQDNKGG
                     LLGTPYDLKNPPAPIYTGGLATFADLTGDGKPELITGRDAFTINWTPGSPPTVSLTLL
                     WKNIDGSNNDGWPAIGDLDGNGTPEVVLVAWKDVKVLDGKTGKLWCGIDPTGVMCEGN
                     DALRTKPIPIEGGNLGGPATIADFDGDGRPEFGVAGGTAYAVYDLYREGEDLVVANGK
                     PMPLKGEIYVRWSQGTQDSTSGCTGSSVFDFQGDGAAEVAYQDECKAYVYDGKTGAKQ
                     LEIPNTSGTVHEYPLVADVDGDGNAEFLTVANPPGMAQVTACQAKTPGLVPRKGVFSY
                     GAGADNWVPTRKVWTQHTYHVTNTDSDGNVPMMEQANWTEPGLNNFRQNVQGEGVFNA
                     ADLTVSLAVDLEGCSEALTLQATIYNEGALGVPAGINVTFYRGMNNQGTNLGTKPTSE
                     ALLTGGSTTVSWPISGKELLPGPQSYFVEVDGGLNSGIIQECKEDNNGALVTEAECPT
                     PG"
     gene            complement(3446..4801)
                     /locus_tag="VGB85_31005"
     CDS             complement(3446..4801)
                     /locus_tag="VGB85_31005"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HEY0138568.1"
                     /translation="MSDAPLDTIVIGAGLAGLTAAQRLRAAGKRVVVLEARDRVGGRT
                     MSVDLAGDTVDLGGQWIGPGQDRVRALANELGVRSFPQYFRGKKILELGAERRTYKGL
                     LPKLPLRALFDLGLSIERLEWMARTIPLEAPGSARKAALWDGMTVADWLDRHVRTADA
                     RKVLELATHAIFAAETRSLSLLYFLFYTRSGRSFTRLAQIQGGAQQERFIGGAQQLSE
                     GLRARIGAEHVLLDSPVTALSHGPEQVVVRTPRADYTARSAVLAVPPALAESIAFSPA
                     LPADRAALHREMPMGSVVKCIVAYDRAFWREAGHSGEVVSDGEPIRAVFDDSSHDGGH
                     PALLCFVLGDVARRFGLLPEGERRAQIVAHLVRLFGDAAASPRAYVDKDWVADPWSSG
                     CYVGLMGPGLLTRAGEALRRPVGRLHFAGTETAVRWCGYLDGAIESGERAATEVLARL
                     A"
     gene            complement(4973..5875)
                     /locus_tag="VGB85_31010"
     CDS             complement(4973..5875)
                     /locus_tag="VGB85_31010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138569.1"
                     /translation="MIAAPEAPLPQPVPGQTPVRVESQVQAPQSQGQAQVQAPGVQNQ
                     VTPGGPQTQQQQFPQLPGGPFPMSPGSINMQPQIQVLVQPQIQVSAQPKGDAAPQAKA
                     HTDVDANTTPTVNSSTSTQASPTITAQPYTPYAPPQVYAPPPPRVITRVVAQPQRYQP
                     YKVIKPLPRRKGLMITGWIVFSVSYLLTASNAADLYDRCPGMSNPGRCKELARDMFIP
                     VAGPFMAMEHTKYATDDYSLAVAGSLQGAGLLMGLIGTSMYVRDGRRNRIINEYGLRV
                     AKNVNVRPNANPLGGGGLVLRGQF"
     gene            6470..7552
                     /locus_tag="VGB85_31015"
     CDS             6470..7552
                     /locus_tag="VGB85_31015"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138570.1"
                     /translation="MTTTIRFVLTGLGNIGRTLLEVLHSRTEVLRERHGVELRAVAVA
                     DASGVALDPRGLDIGAVVALKKQRLGVAMLPGVGRPGVDALELVRSVEADMLLEATPT
                     NHHDGQPGLELVRTALARGLHCVLASKGPLVVAYPELEAASDLGGVAGAPALRFSGAV
                     GGGLPSINIGRRDLAGGRITRVEAVLNGTTQMILGLRARGMSFAQALAEAQRVGIAEP
                     DAELDVQGWDAACKLVILANAVLRRPVKLADVNVTGIAGVTTEALQAAWAGGGRMSLL
                     AVAQALPDPARGYRLSVAPTVLGPDHPLARLGIDEMGIVYYSDIFGRTCAMSLEDGPV
                     GSVAAMLRDILEIAGQARALQAKPGE"
BASE COUNT         1203 a         2642 c         2909 g         1014 t
ORIGIN      
        1 cgctcgccgt gggcctgctc ggcctcgccg gggccccgcc gaccggggcc ttcctcgccc
       61 gcgtcgcctt gctgcaagct ggctttcagg acagcaacct gctcgtccgc gtcgcgctcg
      121 gcgcgggcgc cctcgcgggc gtcgccctcg ccctcgcgtg cctgcgcgtg ctggcgacga
      181 tgtgctcggc gaccggcgac gagccgccgc gcgggcgggc ctgggcgcgg gggacactgc
      241 tcgcgctcgc ggcgatgttg ctggtcaccg ggctgttcgg acaggtgacc gtcgaggccg
      301 cgcgcgcggc cgcggccggt gccgggctgt cgccggggag cccggcgcgc cgcgcatgga
      361 tcgagtcgcg ccagcgcggc ccggatgcac cgcgctgagc tcggtccggg accggctggc
      421 ctttcggcca gatcaccggc gcggcccgag ccgcacgcgg gccgaagata gatcccgctg
      481 tcgcgctgca aacggcgaca ggcgcggcgc agaggtctac ttcacggcaa tgcgcgagaa
      541 gacaccgggc ccgccggccc gtcgctgccg gtccccgcgc gtggcgtggt gaataaaatt
      601 catccggcga cctcgctgtg atgcgcgcct gctatggggg attcgtgacc tgcatcgcga
      661 ggtgacgtga ggacagcata gcgcgccggt aggtcgacac atcgggcccg cgcggccgcc
      721 tcgctcgcgg aaaaaagttg caggacgcgt ggccgggctc gctcctctaa gtctacactg
      781 tcgttatgaa gacctacctt gcgcgttact gggcagtata cggcatctca ctggtggcac
      841 cgggactcgc ggcgtgtggc gactcgacga tggcgaccac cgagggcacg accgagtcgg
      901 gcacgacgga ggtcacaccg acgagctcga cgggcctgac caccacgggt gacgactcga
      961 cctcgaccac ggacacaccg accacgctcg gtagcaactc ggagtccgag agcggctcga
     1021 gctcgacgac cgaagcgacg acgacgacgg gtgacacgac gaccgacgac accaccgggc
     1081 aggtgaccgc gacgaccggc gggacctcga ccggcggccc ggacgtgtgc atgacgatcc
     1141 tgtgcggcga cccggcggtc tgctgcctgg agaccgacga gtgcgtcggc ggcacctgtc
     1201 agccgatctg ccccagcggc gtgcgctgcg gcgccgagca ggagatgtgc tgtgaagaag
     1261 gcgacgtgtg cgtcggcgac acctgcacga cgccgctcgg cgactgcaac gactcgtacg
     1321 actgctcgcc cggcaactac tgcgagccgg tgctcaagaa gtgcctgccg cagcccgacc
     1381 cgctgcagtg cgaggtcgtc ccgaccttcg acaagatcga cgtgcagctc gagtggtcgt
     1441 ggaccgtcaa cgaggtcacc gtcacgccga tggtcgccga tgtccagggc gacgagacgc
     1501 ccgatgtcgt ggtcaacgcg tttcgcaccg acggcatcga ccgcaacctc ggcgagatgg
     1561 tcctgctcga cggcaagacc ggcaccgaga tctggcgcct caagcatgac ccggtgaacg
     1621 gcaccttcgg ctcgttcggc ctcggcaccc cggcgctcgg cgacctcaac ggcgaccaga
     1681 agccggacat cgtgtacccg ggccgctgga cctccaaccc gccgccggcg cccaacgcca
     1741 accccaactc cgagatcggg ccggtgcacg cggtcgatgg cacgggcaag ctgctgtgga
     1801 ccggccacac cgccaacaac ccggcggtca acatccgctg ggcccacgcc gcggcggcga
     1861 tggccaacct cgacgacgac ccggaggccg agatcgcggt cggcggcgcc atcttcgaca
     1921 acgacggcct gctggtgtgg aaccaggaca acaagggcgg cctgctcggc acgccctacg
     1981 acctcaagaa cccgcccgcg ccgatctaca ccggcggtct ggcgaccttc gccgacctga
     2041 ccggcgacgg caagccggag ctgatcaccg gccgcgacgc gttcacgatc aactggaccc
     2101 cgggcagccc gccgaccgtg tcgctgacgc tgctgtggaa gaacatcgac ggctcgaaca
     2161 acgacggctg gccggcgatc ggcgacctcg atggaaacgg cacgcccgag gtggtgctcg
     2221 tcgcctggaa ggacgtcaag gtcctcgacg gcaagaccgg caagctgtgg tgcggcatcg
     2281 acccgaccgg cgtgatgtgc gagggcaacg acgccctgcg caccaagccg atcccgatcg
     2341 aaggcggcaa cctcggcggc ccggcgacga tcgccgactt cgacggcgac ggccggccgg
     2401 agttcggcgt cgccggtggc acggcgtacg cggtctatga cctctaccgc gagggcgagg
     2461 acctcgtggt cgccaacggc aagccgatgc cgctcaaggg cgagatctac gtgcgctggt
     2521 cccagggcac gcaggacagc acctcgggct gcacgggctc gtcggtgttc gacttccagg
     2581 gcgacggcgc ggccgaggtc gcgtaccagg acgagtgcaa ggcctacgtc tacgacggca
     2641 agaccggcgc caagcagctc gagatcccca acaccagcgg caccgtgcac gagtacccgc
     2701 tcgtcgccga cgtcgacggt gacggcaacg ccgagttcct gaccgtcgcc aaccccccgg
     2761 gcatggccca ggtgaccgcc tgccaggcca agacccccgg tctcgtgccg cgcaagggcg
     2821 tgttctcgta cggcgccggc gcggacaact gggtcccgac ccgcaaggtc tggacccagc
     2881 acacgtacca cgtcaccaac accgactcgg acggcaacgt gccgatgatg gagcaggcca
     2941 actggaccga gccgggcctc aacaacttcc gccagaacgt gcagggcgag ggcgtgttca
     3001 acgccgccga cctgaccgtg tcgctggcgg tcgacctcga gggttgctcc gaggcgctga
     3061 ctctccaggc gacgatctac aacgagggcg cgcttggcgt gccggccggc atcaacgtca
     3121 ccttctacag gggcatgaac aaccagggca cgaacctcgg caccaagccg accagcgagg
     3181 cgctgctgac cggcggctcg acgaccgtct cctggcccat cagtgggaaa gagctcctcc
     3241 cgggccccca gagctacttc gtcgaggtcg acggcgggct caacagcggc atcattcagg
     3301 agtgcaagga agacaacaac ggcgcgctcg tgaccgaagc cgagtgcccg actccgggct
     3361 gatgcgtgag gcatgaaccg gcccgcgtgc gggccgggaa agcgggcggg tcgacgcagg
     3421 tcggtccgcc cgcggccgtt cggtgtcagg cgaggcgggc gaggacctcg gtcgcggcgc
     3481 gctcgcccga ctcgatcgcg ccgtcgaggt agccgcacca gcgcacggcg gtctcggtgc
     3541 cggcgaagtg caggcggccg accggacgcc gcagcgcctc gcctgcgcgg gtcagcaggc
     3601 ccgggcccat caggccgacg taacagccgc tgctccacgg gtcggcgacc cagtccttgt
     3661 cgacgtaggc gcgcgggctc gcggccgcgt cgccgaacag gcgcaccagg tgcgcgacga
     3721 tctgggcccg acgctcaccc tccggcagca ggccgaagcg cctggcgacg tcgccgagca
     3781 cgaagcacag cagcgccggg tgcccgccgt cgtgcgagct gtcgtcgaac acggcacgga
     3841 tcggctcccc gtcggacacg acctcgccgg agtggccggc ctcgcgccaa aaggcccggt
     3901 cgtaggcgac gatgcacttg accaccgagc ccatcggcat ctcgcggtgc agcgcggcgc
     3961 ggtcggccgg cagcgcgggc gagaaggcga tcgactcggc gagcgccggc gggaccgcga
     4021 gcacggcgct gcgggcggtg tagtcggcgc ggggcgtgcg gacgacgacc tgctcggggc
     4081 cgtgcgagag cgcggtgacc gggctgtcca gaaggacatg ttcggcgccg atacgggcgc
     4141 gcaggccctc gctgagctgc tgcgcgccgc cgatgaagcg ctcctgctgc gcccccccct
     4201 ggatctgcgc gaggcgggtg aagctgcggc ccgagcgggt gtagaacagg aagtagagca
     4261 gcgacagcga gcgcgtctcg gcggcgaaga tcgcgtgggt cgcgagctcg agcaccttgc
     4321 gcgcgtcggc ggtcctgacg tggcgatcca gccagtcggc gacggtcatg ccatcccaca
     4381 gggcagcctt gcgggcgctg ccaggcgcct cgagggggat cgttcgcgcc atccactcga
     4441 ggcgctcgat cgacagcccg aggtcgaaca gggcccgcag cggcagcttg ggcagcaggc
     4501 ccttgtaggt gcgccgctcg gcgccgagct cgaggatctt cttgccgcgg aagtactgcg
     4561 ggaagctgcg cacgccgagc tcgttcgcca gggcccgcac gcggtcctgg ccgggcccga
     4621 tccactggcc gccgaggtcg acggtgtcgc cggcgaggtc gacggacatc gtgcgcccac
     4681 cgacgcggtc gcgggcctcg agcacgacca cccgcttgcc ggccgcgcgc aggcgctgcg
     4741 cggcggtcag gccggcgagg ccggcaccaa tcacgatcgt gtcgaggggg gcgtcgctca
     4801 cggcgcggga cggtagcagc cggcgacccg cgaggcgaga gggattacct gtcttttggg
     4861 acagcctcgc tcctcgcagg cattaaacgc cgacgggcgc ggtggtcgac cgcgcccgca
     4921 ggcttcgtgt ccgttccccg aagggaacct cccccggagg gagaagcgag gatcagaact
     4981 ggccgcgcag gaccaggccg ccgccgccga gcgggttggc gttggggcgc acgttgacgt
     5041 tcttggcgac gcgcaggccg tactcgttga tgatgcggtt gcgacggccg tcgcggacgt
     5101 acatcgaggt gccgatcagg cccatgagga ggccggcgcc ctgcagcgag ccggcgaccg
     5161 cgagcgagta gtcatcggtg gcgtacttgg tgtgctccat cgccatgaac ggaccggcga
     5221 cggggatgaa catgtcgcgg gcgagctcct tgcagcggcc ggggttgctc atgccggggc
     5281 agcggtcgta gaggtccgcg gcgttgctgg cggtcagcag gtacgagacg gagaacacga
     5341 tccagccggt gatcatcagg cccttgcggc gcggcagcgg cttgatcacc ttgtagggct
     5401 ggtagcgctg cggctgggcg acgacgcggg tgatcacgcg cgggggcggc ggcgcgtaca
     5461 cctgcggggg cgcatacggc gtgtacggct gggcggtgat cgtcgggctc gcctgcgtgc
     5521 tcgtcgagct gttgacggtg ggggtggtgt tggcgtcgac gtcggtgtgc gccttggcct
     5581 gcggggcggc gtcacccttg ggctgggccg agacctggat ctgcggctgg acgagcacct
     5641 ggatctgcgg ctgcatgttg atcgagccgg ggctcatcgg gaacggaccg ccaggcagct
     5701 gcgggaactg ctgctgctgg gtctgcggac cgccgggagt gacctggttc tgcacgccgg
     5761 gggcctggac ctgcgcctga ccctgggatt gcggggcctg gacctgggac tccacgcgga
     5821 ccggggtctg gccgggtacg ggctggggca gcggcgcctc cggggcggcg atcactggac
     5881 cgacggggag ggtctcctgg gcgagaaccg tcgtgggcag gagcagagca cacgcgacga
     5941 tgggagagac gagacgagcg acaggaagaa gacggttccg cagcatggcg tggggcctat
     6001 cctcggatgg cggggaggtg tgcacggtcg gtcggaccaa caagcggcgg ccgagtcgac
     6061 gccgctcaca cgggaatcaa tgtggcagat gcagtacgga gcgaccagac aaaaagtcat
     6121 acgatcgtat ggcggtccag gcgcaaaatc ccgccgtgct gggagctatc gcgtgttcga
     6181 ctgtcgcggg ttcgaatttg ccggtggccg gcctcgcgcc aggacgcaca cgatcgccgt
     6241 gaaaggggca agaaaccggc gaattccggg ttccacgaga gtgggggcga gaccccgcag
     6301 gcggcggcgg gcgtgaccgg gcgcgggcgg acggggccgg tgcgatcgga cccgggccgg
     6361 acgtcgcagg gtcgatcgcc cggcgaggat cggtggcccg gcgtgcgcgg cgggcgacct
     6421 cgtccccggc cttgagcggg ccacccgcga tcgggtacga agaccggaag tgacgaccac
     6481 gatccgcttc gtcctgaccg ggctcgggaa catcgggcgc acgctgctcg aggtcctcca
     6541 ctcgcgcacc gaggtcctgc gcgagcgcca tggcgtcgag ctgcgggccg tcgcggtcgc
     6601 cgacgcgtcc ggggtcgcgc tcgacccccg cggcctcgac atcggcgccg tcgtggcttt
     6661 aaaaaagcag cgcctcgggg tcgccatgct gccgggggtc gggcggcccg gggtcgacgc
     6721 gctcgagctg gtgcgctcgg tcgaggccga catgttgctc gaggcgacgc ctaccaacca
     6781 tcacgacggg cagccgggcc tcgagctggt gcgcacggcg ctggcgcggg ggctgcactg
     6841 cgtgctcgcc agcaaggggc cgctggtggt cgcgtacccc gagctcgagg cggcgagcga
     6901 cctcggcggg gtcgctgggg cgccggcgct gcgcttctcg ggggcggtcg gcggcggact
     6961 gccgagcatc aacatcggtc gccgcgacct cgcgggcggg cgcatcaccc gggtcgaggc
     7021 ggtgctcaac ggcaccacgc aaatgatcct cgggctgcgc gcgcgcggga tgagcttcgc
     7081 gcaggcgctc gcggaggccc agcgggtcgg catcgccgag cccgacgccg agctcgatgt
     7141 ccagggctgg gacgcggcgt gcaagctcgt catcctggcc aacgcggtgc tgcggcgtcc
     7201 tgtcaagctc gcggacgtca acgtcacggg gatcgcaggg gtgacgaccg aggcgctgca
     7261 ggccgcgtgg gcgggcggcg gccgcatgtc gctcctggcg gtcgcgcagg cgctgcccga
     7321 cccggcgcgg ggctatcggc tgagcgtggc gccgacggtg ctcgggcccg accatccgct
     7381 cgcgcgcctc gggatcgacg agatgggcat cgtctattac agcgacatct tcggccgcac
     7441 ctgcgcgatg agcctcgaag acggcccggt cggctcggtc gcggcgatgc tgcgcgacat
     7501 cctcgagatc gcaggtcagg cgcgggcgct tcaggcgaag ccgggggagt aaacaggcga
     7561 ggccgggggg cgcttcaggc gaagccgggg gcgcttcagg cgaagccggg gggcacttca
     7621 ggcgaagccg ggggagtaaa caggcgaagc cgggggcgct tcaggcgaag ccgggggcgt
     7681 aaacagcggc ccactggcgc gggcggacga tcgcgtggtc gatcatcgtg cggtcggcct
     7741 cggcgaccag cgcggcgccg gccggtcc
//
LOCUS       DASWNJ010001859         1826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_137480, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001859 DASWNJ010000000
VERSION     DASWNJ010001859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137480"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VGB85_31020"
     CDS             <1..554
                     /locus_tag="VGB85_31020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138571.1"
                     /translation="LHPDSHPKILISDTVGFIQKLPHDLVASFRSTLDEARDASLLLY
                     VVDASDPTFRDEYEVTRTVLREIEADASPSLLILNKIDRVDPEQREALRAEYPDAIQL
                     SALDREDVARLRTIIIEAVAKTLTEAELFVPYNQHGVTAALHEHGQVLTEKHEDEGTR
                     LTVRAPAELLERLKAQLAEGAAS"
     gene            complement(556..1737)
                     /locus_tag="VGB85_31025"
     CDS             complement(556..1737)
                     /locus_tag="VGB85_31025"
                     /inference="COORDINATES: protein motif:HMM:NF019436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138572.1"
                     /translation="MTDDRRERLDHLRGALQLLGAGIEAGTEIVEDMHRAIAATPLAA
                     LDAVSERRISRVHDDVSRGVYAWIRGVSRLVFRAADLALAHAGPALAPPAIPGPLVGV
                     LHGILGDGMERDENPLRIAMHLRHDGRRVPVTPEALAGLLPANCPRLVVFVHGLACDE
                     SSWEHASVKAWGRPGVSYAALLRAQGLATLHVRYNSGLRVDANGRTLAALLGELLAVH
                     PVNDLVLVGHSMGGLVVRSACHHGRGQAWAGHVRDVVCLGSPHGGAPLEQLGVLATTL
                     LRAVPVTAAIGRAADRRSAGIKDLGRGTVVADQPCDEPLPHARYHFIAGSYGATARTR
                     ALGWAFGDGLVRVASARHDRGAHDRVVRVHLPGLHHMHLLNHPRVFAHLEATLLRSLV
                     P"
BASE COUNT          267 a          630 c          695 g          234 t
ORIGIN      
        1 cgctacatcc cgacagccac ccgaagatcc tgatctcgga cacggtcggg ttcatccaga
       61 agctcccgca cgacctcgtg gcgagctttc gcagcaccct cgacgaggcg cgcgacgcct
      121 cgttgctgct ctacgtcgtc gacgcgagcg acccgacctt tcgcgacgag tacgaggtga
      181 cgcgcacggt gttgcgcgag atcgaggccg acgcctcgcc ctcgctgctg atcctcaaca
      241 agatcgaccg cgtggacccc gagcagcgtg aggccctgcg cgccgagtac ccggacgcga
      301 tccagctctc ggcgctcgac cgcgaggatg tcgcgcgcct gcggacgatc atcatcgagg
      361 cggtggcgaa gacgctgacc gaggccgagc tgttcgtgcc gtacaaccaa cacggcgtga
      421 ccgcggcgct gcacgagcac ggccaggtat tgaccgagaa gcacgaggac gagggcacgc
      481 gcctgaccgt gcgcgccccc gccgagctgc tcgagcgcct caaggctcag ctcgccgagg
      541 gcgccgcgtc ctgattcacg ggacgagcga gcgcagcaag gtcgcctcca ggtgtgcgaa
      601 cacccggggg tgattgagca ggtgcatgtg atgcaggccg ggcaggtgca cgcggacgac
      661 ccggtcgtgc gcgccgcggt cgtgacgggc gctggcgacc cgtacgaggc cgtcgccgaa
      721 ggcccagccg agcgcgcgcg tgcgggcggt cgcgccgtag ctgccggcga tgaagtggta
      781 gcgggcgtgg ggcaggggct cgtcgcaggg ctggtcggcg acgacggtgc cgcggccgag
      841 gtccttgatg ccggcgctgc gccgatcggc ggcgcggccg atcgcggcgg tcaccggcac
      901 ggcgcgcagc agcgttgtgg cgagcacgcc gagctgctcg agcggcgcgc cgccgtgcgg
      961 tgaaccgagg cacacgacgt cgcggacgtg cccggcccac gcctggccgc ggccgtgatg
     1021 acaggcgctg cgcacgacca gaccgcccat gctgtgaccg acgaggacca ggtcgttgac
     1081 gggatggacc gcgagcagct cgccgagcag cgccgcgagg gtccggccgt tggcgtcgac
     1141 gcgcaggccg ctgttgtagc gcacgtgcag ggtcgccagg ccctgcgcgc gcagcagcgc
     1201 ggcgtagctg acgccggggc ggccccacgc cttgaccgac gcgtgctccc aggaggactc
     1261 gtcgcaggcg aggccgtgca cgaacacgac gaggcgcggg cagttcgccg ggagtagccc
     1321 ggcgagcgcc tcgggggtca ccggcacacg ccgaccgtcg tgacgcaggt gcatggcgat
     1381 ccgcagcggg ttctcgtcgc gctccatgcc gtcgccgagg atcccgtgca gcacgccgac
     1441 gagcgggccg gggatcgcgg gcggtgcgag ggccgggccg gcgtgcgcga gcgcgaggtc
     1501 ggcggcgcga aacaccagcc ggctgacgcc gcggatccag gcataaacac cgcgagacac
     1561 gtcgtcgtgc acgcggctga tccggcgctc gctgacggcg tcgagggcgg cgagcggcgt
     1621 ggcggcgatc gcccggtgca tgtcctcgac gatctcggtg ccggcctcga tgccggcgcc
     1681 gagcaattgc agggcgccac gcaggtggtc gaggcgttcg cggcggtcgt cggtcatttt
     1741 cagctgtcct ttttgactcg tggcttgcgc ttgcggctcg ccggcagcgc ggcgagcttg
     1801 ccggacgcca tcagggcccg cagcga
//
LOCUS       DASWNJ010001860         1828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_104346, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001860 DASWNJ010000000
VERSION     DASWNJ010001860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104346"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VGB85_31030"
     CDS             <1..975
                     /locus_tag="VGB85_31030"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEY0138573.1"
                     /translation="TVAQFAHEVEGFLFKGRDAERRYHETNHFDFISTGGYYHSLPGD
                     PLRRFIDSAAEVQRAMGFGNEKDHPEVAPSQFEMNFSHTEACVAADQVQLYKLLCRQV
                     AQNFDMSASFLPKPVTGINGNGMHTNISLLRDKNNLFHDKAGESGLSALGWNFTERIL
                     NSASDICLVLNPSVNAYRRLDPHFEAPNQIKASASDRGSMVRIPIANERSSRIEVRSV
                     APDVNPYMTVYTLLRCGLEGPQPDAAEVASKRARTRFLPDNINDAIRLFKSSRVVAEL
                     LGEGVQSRFAEVKQASAERCPRALGTMIKSAEVQFHHEVTNQWLWNQF"
     gene            complement(1123..>1828)
                     /locus_tag="VGB85_31035"
     CDS             complement(1123..>1828)
                     /locus_tag="VGB85_31035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138574.1"
                     /translation="VLAACAVFMLALGRTADVVFAHAHNFVAVGVWWLWRPRLGRAHW
                     VPLALLMLTTIFLLAGPALQLAHASGGLDWFGAGMGPDYQLWRLAPDLAPGPGLRLVL
                     LFCFAQTIHYALWLQLIPDEDRARPTPTTFRASHEALCKDLGDAGLIVAALLAVGVAI
                     WAVADLGAANHGYFRMARFHGHLEIMAATLLLLEGRRRATTPRRRTSSRPPTTGPSRA
                     RPDDRHEQHDRYQRHE"
BASE COUNT          315 a          607 c          641 g          265 t
ORIGIN      
        1 acggtggcgc agttcgccca cgaggtcgag ggcttcctgt tcaagggccg cgacgccgag
       61 cgccggtacc acgagaccaa ccacttcgac ttcatctcga ccggcggtta ctaccactcg
      121 ctgcccggcg acccgctgcg ccgcttcatc gactcggcgg cggaggttca gcgcgcgatg
      181 ggctttggca acgagaagga ccacccggag gtggcgccct cgcagttcga gatgaacttc
      241 tcgcacaccg aggcctgcgt cgcggcggat caggtccagc tttacaagct tctgtgccgg
      301 caggtcgcgc agaacttcga catgagcgcg agctttttgc ccaagccggt cacggggatc
      361 aacggcaacg ggatgcacac caacatctcg ctgctgcgcg acaagaacaa cctgttccac
      421 gacaaggccg gcgagagcgg gctgtcggcg ctcggctgga atttcacgga gcggatcctc
      481 aacagcgcgt ccgacatctg tctggtcctc aatcccagcg tcaacgcgta ccgccggctc
      541 gacccgcact tcgaggcgcc gaaccagatc aaggccagcg cgagcgaccg ggggtcgatg
      601 gtgcggatcc cgatcgccaa cgagcgctcg tcacggatcg aggtccgcag cgtcgcgccg
      661 gacgtcaacc cgtacatgac cgtgtacacg ctgctgcgct gcggcctcga gggaccgcag
      721 ccggacgccg ccgaggtcgc cagcaagcgc gcgcgcaccc gcttcctgcc cgacaacatc
      781 aacgacgcga tccgcctgtt caagtcgagc cgcgtcgtcg ccgagctgct cggcgagggc
      841 gtgcagtcgc ggttcgcgga ggtcaagcag gcgtccgccg agcgctgccc gcgggcgctc
      901 gggacgatga tcaagtcggc cgaggtgcag ttccaccacg aggtgacgaa ccagtggctg
      961 tggaaccagt tctgagcgct ctgctcacca cggcatgcgc tcgaagcaag cgcacatatt
     1021 gaagtcgccg acgcactgac cagttcgcca aagtgcgtcc aggtctgcgc gcaccgcggt
     1081 ccgcaggtcg aggcgccgtc ggtgccgccg gtgtcgccgg tgtcactcgt gtcgctggta
     1141 tcggtcgtgc tgctcgtgcc ggtcgtcagg tcgggcccgt gatggtcccg tcgtcggtgg
     1201 ccgtgagctg gtccgtcgtc gaggggtcgt cgctcgtcgc cggccttcga gcagcagcag
     1261 ggtcgcggcc atgatctcga ggtggccgtg gaacctggcc atgcgaaagt agccgtggtt
     1321 ggcggcgccg aggtcggcga ccgcccatat ggcgacgccg accgcgagca gggcggcgac
     1381 gatcaagccg gcgtcaccga ggtccttaca taaagcttcg tggctggcgc gaaaggtcgt
     1441 cggggtcggg cgcgcgcggt cctcgtcggg gatcaactgc aaccataatg cgtagtggat
     1501 cgtctgcgcg aagcagaaca gcaggaccag gcgcaggcca gggccgggcg cgaggtccgg
     1561 cgcgaggcgc cacagctgat aatcggggcc catgccggcg ccgaaccagt cgaggccgcc
     1621 gctggcgtgc gcgagctgca gggccgggcc ggcgagcagg aagatcgtgg tgagcatcaa
     1681 caatgccagc gggacccagt gggcccgccc gagccgcggc cgccataacc accacacgcc
     1741 gacggcgacg aagttgtgcg cgtgggcgaa gacgacgtcg gcggtgcgcc cgagcgcgag
     1801 catgaagacg gcgcaggcgg cgagcacg
//
LOCUS       DASWNJ010001861         4673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_317043, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001861 DASWNJ010000000
VERSION     DASWNJ010001861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317043"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1886)
                     /locus_tag="VGB85_31040"
     CDS             complement(60..1886)
                     /locus_tag="VGB85_31040"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0138575.1"
                     /translation="MYLFSRPTHRALRGISAPLTLSLLLATACGDDGTATGEDDTGTD
                     DGADESDSDQTPTTAGPPGPPGEPGEPGEPGDPGDPGAPGEPGEPGEPGEPGEPGEPG
                     EPGAPGEDGNVDPNLSPLEKAYVGMGGKDAVAGLTAFTIEASGSRYMPGEGFNPGDPA
                     QYVGDFTATVNYDVADANLRIDYVRDIAFLGGMFNTTFSEILAGDLGYVDGTESIFGA
                     PAGDMQSDRWAAILRQQRLLNPHLILQDIIADETIAKEGGVQLYDGRVYNLLVVDDPV
                     APLTLFVDNLTGKLTKIETIENDYLHRDVELSVFYNGWQSTTDGPRFPHEVFIAVEEN
                     ILHHEKRGTITVGGPLDSALFEFPDGAMPMFVAEDAARGAATHQFHETFAAVGIPLDG
                     LQTFVMANELAPGVWHLTGGSHNSMVVEQEDGIVVIEAPLYEYRSEAILDWIEMNLPG
                     KPVTHVLATHHHDDHTGGLRTFVAAGVEVVIGEAAEKFFSEIFAAPSTVIPDALSIAP
                     KKANITTVPQGDAITLDDATHPVVAQAITSFHAKDMLIFYLPSDGVVFVSDIYSPGQP
                     PNPFGAAEVYNAIIDNALDVTTLAGGHGGVGTFDELEMIVMP"
     gene            2084..2860
                     /locus_tag="VGB85_31045"
     CDS             2084..2860
                     /locus_tag="VGB85_31045"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY0138576.1"
                     /translation="MFAPEIPAWPLGDPIAGPIVLVLAGVLAGVANTLAGGGSFIILP
                     ILIGLGLSPGVANATSRVGVLAHGSAATITFARARAVPTGLVIRVAPTMCVGALLGAW
                     LATRLDDALLRPLFGAILVLWSIVLIVRPGRFIKPPEQPLTPGPLAQALSLLIGVYGG
                     FLQAGVGFPLMALFVSMLGYPTLQANAAKIAVVFIYTLMAMSVFMAAGQVAWREGAIL
                     AVGMILGGVLGAQLHLRAGAVVVRWAMVVMVAISGIALLV"
     gene            complement(2872..3534)
                     /locus_tag="VGB85_31050"
     CDS             complement(2872..3534)
                     /locus_tag="VGB85_31050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138577.1"
                     /translation="MAYRASSTLALLLLAACGPGTDDVSATASTTDTSGDTTTGTTTN
                     PTTGPTTGQTTGVTTEATTEATTGQTSDPTTGATGQTTDATTGDEVCPDQFPQDGTPC
                     TSEGLSCSTGCEDPCEFCNVVQCENGTWQNLEVFPYDCLDCESVCEFVVPAACAGGPP
                     DQAACVAGCMDNLAGDCKLVFNQMLGCIGSMPTFTCNAETRPTVAGCEDRFTELYACM
                     GF"
     gene            complement(3578..>4673)
                     /locus_tag="VGB85_31055"
     CDS             complement(3578..>4673)
                     /locus_tag="VGB85_31055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138578.1"
                     /translation="AIAGLLVYTDFYSSTLSGVGLTGDPVTLGDTGIRGLVTFAEAAD
                     GEMYALDYQGGIYKLVAAEPRTGPGLPARLSETGCMDAPGTLPASAIPYEINHPFWSD
                     GAAKQRWMVLPGTSKLLVTPAGDFDFPGGTVLVKHFELGETPVETRLLVRHDDGAWAG
                     YSYAWDETGTDATLLTTGETRTIAGQSWIYPGRDECLYCHTEAAGRTLGLEAAQLDRT
                     ITGPDGAPINQLDHFVNLGLLIARPEATPLPWLTDDAPLADRARAYFHANCSQCHRPE
                     APGGRARIDFRVDTPLAEVGICDAAPRSGDLDIADARLVVPGDPARSIVSARMHTLGS
                     TRMPALGSAVVDPTGTEVVDAWISSLGACP"
BASE COUNT          642 a         1438 c         1827 g          766 t
ORIGIN      
        1 ccgcgggtgt caagcgcggc gcggcggacg agttcgcgcg gagggccgca cgaaagtgat
       61 cacggcatga cgatcatctc gagctcgtcg aaggtcccga cgccgccgtg tccgcccgcg
      121 agggtcgtga catcgagggc gttgtcgatg atcgcgttgt agacctcggc cgcgccgaac
      181 gggttcggcg gctgacccgg gctgtagatg tcgctgacga acacgacgcc gtcgctcggc
      241 aggtagaaga tcagcatgtc cttggcgtgg aacgaggtga tcgcctgcgc gaccacgggg
      301 tgcgtcgcgt cgtcgagggt gatcgcgtcg ccctgcggca cggtcgtgat gttcgccttc
      361 ttcggggcga tcgagagcgc gtcggggatc accgtcgacg gggccgcgaa gatctcggag
      421 aagaacttct cggcggcctc gccgatgacg acctcgacgc cggcggccac gaaggtccgc
      481 agaccgccgg tgtggtcgtc gtggtggtgc gtggcgagca cgtgggtgac cggcttgccc
      541 ggcaggttca tctcgatcca gtcgaggatc gcctcgctgc ggtactcgta gagcggcgcc
      601 tcgatcacga cgatgccgtc ctcctgctcg acgaccatcg agttgtgcga gccgccggtc
      661 aggtgccaga cgccgggcgc gagctcgttg gccatcacaa aggtctgcag gccgtcgagc
      721 gggatgccga ccgcggcgaa ggtctcgtgg aactgatggg tcgccgcgcc gcgggcggcg
      781 tcctcggcga cgaacatcgg catcgcgcca tcggggaact cgaacagggc gctgtcgagc
      841 gggccgccga cggtgatcgt gccgcgcttc tcgtggtgca ggatgttctc ctcgaccgcg
      901 atgaagacct cgtgcgggaa gcgcgggcca tcggtggtcg actgccagcc gttgtagaac
      961 acgctcagct cgacgtcgcg gtgcaggtag tcgttctcga tcgtctcgat cttggtcagc
     1021 ttgccggtca ggttgtcgac gaagagggtc agcggggcga ccgggtcgtc gacgaccagc
     1081 aggttgtaga cgcgaccgtc gtacagctgg acaccgccct ccttggcgat cgtctcgtcg
     1141 gcgatgatgt cctggaggat caggtgcggg ttgagcagcc gctgctggcg aaggatcgcg
     1201 gcccagcggt cggactgcat gtcgccggcc ggcgccccga agatgctctc ggtgccgtcg
     1261 acgtagccga ggtcgcccgc gaggatctcg ctgaaggtgg tgttgaacat gccgccgagg
     1321 aaggcgatgt cgcggacgta gtcgatgcgc aggttggcgt cagcgacgtc gtagttgacg
     1381 gtcgcggtga agtcgccgac gtactgcgcg gggtcgccgg ggttgaagcc ctcgcccggc
     1441 atgtaacggc tgccgctcgc ctcgatcgtg aaggcggtca ggccggcgac ggcatccttg
     1501 ccgcccatgc cgacgtaggc cttctcgagc gggctcaggt tggggtcgac gttgccgtcc
     1561 tcgccggggg caccgggctc gccgggttcg ccgggctcac cgggctcgcc gggctcaccg
     1621 ggctcaccgg gctcaccggg ggcgccgggg tcaccggggt caccgggctc accgggctcg
     1681 ccgggctcgc cgggagggcc gggagggccc gcggtggtcg gcgtctgatc cgagtcactc
     1741 tcgtcggcgc cgtcgtcagt gccggtgtcg tcctctccgg tcgcggtgcc gtcgtcgcca
     1801 caggcggtgg cgagcagcag ggagagcgtc aggggcgcgg agatgccacg cagcgcacgg
     1861 tgggtggggc gcgaaaacaa gtacattatc aaatccttgc aggagtaagg cgcagcgaaa
     1921 cagccacacc ggccgccgac cttacaggca atgtgcgcgc gagggtggcc atcgccatgc
     1981 accgtgcgca tgacgaacct gcacgcgccg catgatgatc ggcggatcaa tgtgggtgat
     2041 gcgcggtgat gcatcgcgcg gtgcgatcgc gtagagtggg cgagtgttcg cgcccgaaat
     2101 acctgcctgg ccgctcggcg atccgatcgc ggggccgatc gtgctcgtgc tcgcgggggt
     2161 gctcgcgggg gtcgccaaca cgctcgccgg gggcgggtcg ttcatcatct tgccgatcct
     2221 gatcggcctc ggcctcagcc cgggggtcgc caacgcgacg agccgcgtcg gtgtgctcgc
     2281 ccatggatcc gccgcgacga tcaccttcgc gcgcgcgcgt gcggtgccga cggggctggt
     2341 gatccgcgtc gcgccgacga tgtgcgtcgg cgcgctgctc ggcgcgtggc tggcgacgcg
     2401 actcgacgat gccctgctgc gcccgctgtt cggggcgatc ctcgtgctct ggtcgatcgt
     2461 gttgatcgtt cggccggggc gcttcatcaa gccgcccgag cagccgctga cgcccgggcc
     2521 gctcgcgcag gcgctgtcgc tattgatcgg ggtgtacggc gggttcttgc aggcgggcgt
     2581 cggcttcccg ctgatggcgc tgttcgtgtc gatgctcggc tacccgacgc tccaggccaa
     2641 cgcggccaag atcgcggtcg tgttcatcta cacgctgatg gcgatgagcg tgttcatggc
     2701 ggccgggcag gtcgcctggc gcgagggggc gatcctcgcg gttggcatga tcctcggcgg
     2761 cgtgctcggg gcccagctgc acctgcgcgc cggggcggtg gtcgtacgct gggcgatggt
     2821 cgtgatggtc gcgatcagcg ggatcgcgtt gctcgtgtag cgaggcgttt tttaaaagcc
     2881 catgcaggcg tagagctcgg tgaagcggtc ctcgcagccg gcgacggtcg ggcgggtctc
     2941 ggcgttgcag gtgaaggtcg gcatcgaacc aatgcagccg agcatctggt tgaacaccag
     3001 cttgcagtcg ccggcgaggt tgtccatgca accggcgacg caggcggcct ggtcgggcgg
     3061 gccgccggcg caggcggccg ggacgacgaa ctcgcagacg ctctcgcagt cgaggcagtc
     3121 gtacgggaac acctccaagt tttgccaggt gccgttctcg cactgcacga cgttgcagaa
     3181 ctcgcacggg tcctcgcagc cggtgctgca cgacaggccc tccgaggtgc acggcgtgcc
     3241 gtcctgcggg aactggtccg ggcagacctc gtcaccggtg gtggcatcgg tggtttggcc
     3301 agtggccccg gtggtcgggt cactggtctg gccggtggtg gcctcggtgg tggcctcggt
     3361 ggtcaccccc gtcgtttggc cggtggtcgg gccagtggtc gggttcgtgg tggtgccggt
     3421 ggtggtgtcg ccggaggtat cggtggtgga ggcggtggcg gagacgtcgt cggtgccggg
     3481 gccgcaggcg gccaggagga gcagggcgag tgtggaagaa gcgcggtaag ccatgggccg
     3541 ggagactaac cgcgtcggac gcgacgagca atgcagctca tgggcacgcg ccgaggctcg
     3601 agatccacgc gtcgacgacc tcggtgccgg tcgggtcgac gacggcggag ccgagggcag
     3661 gcatgcgcgt gctgccgagg gtgtgcatgc gcgccgagac gatcgatcgc gcggggtcgc
     3721 cgggcacgac caggcgggcg tcggcgatgt cgaggtcgcc cgagcgcggg gcggcgtcgc
     3781 agatcccgac ctcggcgagg ggagtgtcga cgcggaagtc gatgcgcgcg cgaccgccgg
     3841 gggcctcggg gcggtggcac tgcgagcagt tggcgtggaa gtaggcgcgg gcgcggtccg
     3901 cgagcggggc gtcgtcggtg agccacggca gcggggtcgc ctcggggcga gcgatcaaca
     3961 ggccgaggtt cacgaaatgg tcgagttggt taatgggcgc gccgtcgggg ccggtgatcg
     4021 tgcggtcgag ctgcgcggcc tcgaggccga gcgtgcgacc ggcggcctcg gtgtgacagt
     4081 agaggcactc gtcgcggccg gggtagatcc agctctggcc ggcgatcgtg cgcgtctcgc
     4141 cggtggtcag cagggtcgcg tcggtgccgg tctcgtccca cgcatagctg tagccggccc
     4201 aggcgccgtc gtcgtggcgc acgagcagcc ttgtctcgac gggggtctcg ccgagctcga
     4261 agtgcttgac gagcacggtg ccgccgggaa aatcgaagtc ccccgcgggc gtgacgagga
     4321 gcttgctggt cccgggcagg accatccagc gctgcttggc ggcgccgtcg gaccagaagg
     4381 gatggttgat ctcgtagggg atcgcggacg cggggagggt gcccggcgcg tccatgcagc
     4441 cggtctcgga caggcgcgcg ggcaggccag ggcccgtgcg cggctcggcg gcgacgagct
     4501 tgtaaatgcc gccttgataa tcaagcgcgt acatctcacc gtcggcggcc tcggcgaagg
     4561 tgacgaggcc gcggatgccg gtgtcgccga gcgtcacggg gtctccggtg aggccgacgc
     4621 ccgacagggt gctgctataa aagtcggtat atacgagcag gccggcgatc gcg
//
LOCUS       DASWNJ010001862         2086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_137497, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001862 DASWNJ010000000
VERSION     DASWNJ010001862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137497"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /locus_tag="VGB85_31060"
     CDS             <1..1181
                     /locus_tag="VGB85_31060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138579.1"
                     /translation="EKEKSLEIERKNIAEVVRERVSVEKGVAEEEEKTKDIRILADAN
                     RTKDARVIKAEGEAQERVIKDVKTAQAGEEIAKFEAKKQLIHAEAGLEAADKLAKAKI
                     RAAEGQQAEAAAAGLAEVRVKEADASAIEKLGMAKIREQEAEAGAIEKRGMAENTVIR
                     ERYSIEAAGEAQKGLAAARVQEAEAGALEKRGAAQASADEKHGVALAVAAERQGVAAA
                     VAVREKLLAEATGLAEKAKAMKAMEGTPREHEEFRLELERQKEVQLAQIAASRVLAEE
                     QGKVLAVAMQHAKINIVGGDGAFFDRFIRAVGTGQAIDGTINNSETLKKLLGEYLDGQ
                     KSLPADLKEILSRPAVDADGVQKLTVSALLGDLMLSSDGAVKRKLQALLQKAQELGLE
                     "
     gene            1185..>2086
                     /locus_tag="VGB85_31065"
     CDS             1185..>2086
                     /locus_tag="VGB85_31065"
                     /inference="COORDINATES: protein motif:HMM:NF023874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair ATPase"
                     /protein_id="HEY0138580.1"
                     /translation="MADGKDTKSAPATTGSEGLDGGSYEVIRARLVAQAKDLADRTHA
                     LNARRQKTFGGTELAVVANERVRTENNCVPADILQVGGHLLLGYNVFFGLKKEVALED
                     VFSLQSFDPNAPEGQGINPVAPGQVPGLLDGEGFLKEFADLYRYYRDTKLRRLKATEN
                     KLFAVFQVSAALEDVKIFHWNVLPNGSVRYVDNRGDREFPRPASHDFEWTNTTRDDHV
                     SGPHPHVNIKDLVFVETVNGDLTIKVENNTKDGLGLYREPVDDANQALDDAKILYAIM
                     GPLVLLKILPYRELKWRYFIYNTRV"
BASE COUNT          407 a          678 c          746 g          255 t
ORIGIN      
        1 tcgagaagga gaagtccctc gagatcgagc gcaagaacat cgccgaggtc gtgcgcgagc
       61 gcgtctcggt cgagaagggc gtcgccgagg aggaggagaa gaccaaggac atccggatcc
      121 tcgccgacgc caaccgcacc aaggacgccc gcgtcatcaa ggccgagggc gaggcgcagg
      181 agcgggtgat caaggacgtg aagacggccc aggccggcga ggagatcgcc aagttcgagg
      241 ccaagaagca gctgatccac gccgaggccg ggctcgaggc cgccgacaag ctcgccaagg
      301 ccaagatccg cgccgccgag ggccagcagg ccgaggccgc cgcggcgggc ctcgccgagg
      361 tccgcgtgaa ggaggccgac gcgagcgcga tcgagaagct cggcatggcg aagatccgcg
      421 agcaggaggc cgaggccggc gcgatcgaga agcgcggcat ggccgagaac acggtcatcc
      481 gcgagcgcta cagcatcgag gccgccggtg aggcgcaaaa gggcctcgcc gccgcccgcg
      541 tgcaggaggc cgaggcgggc gcgctcgaga agcgtggcgc ggcgcaggcg agcgcggacg
      601 agaagcacgg cgtcgccctc gcggtggcgg ccgagcgtca gggcgtcgcc gcggcggtgg
      661 ccgtgcgcga gaagctgctc gccgaggcga ccggtctggc cgagaaggcc aaggcgatga
      721 aggcgatgga gggcacgccg cgcgagcacg aggagttccg cctcgagctc gagcgccaga
      781 aggaggtcca gctggcccag atcgccgcct cgcgcgtgct ggcggaggag cagggcaagg
      841 tgctggccgt ggccatgcag cacgccaaga tcaacatcgt cggcggcgac ggggcgttct
      901 tcgaccgctt catccgcgcg gtcgggaccg gccaggcgat cgacggcacg atcaacaaca
      961 gcgagaccct caagaaattg ctcggcgagt acctcgacgg ccagaagagt ctccctgcag
     1021 acctcaagga aattctctcg cggccggcgg tcgacgccga cggcgtgcag aagctgacgg
     1081 tctcggcgct gctcggcgac ctgatgctgt cctcggacgg cgccgtcaag cgcaagctgc
     1141 aggccctgct gcagaaggcg caagagctcg ggctcgagta aatcgtggcg gacggaaagg
     1201 acacgaagtc cgcgccggcg acgaccggca gcgagggtct cgacggcggc agctacgagg
     1261 tgatccgcgc gcgcctggtc gcgcaggcca aggacctcgc cgaccgcacg cacgccctca
     1321 acgcgcgccg ccagaagacc tttggtggca ccgagctggc ggtcgtcgcc aacgagcgcg
     1381 tgcgcaccga gaacaactgc gtgccggccg acatcttgca ggtcggcggg cacctgctgc
     1441 tcggctacaa cgtgttcttc gggctcaaga aggaggtcgc gctcgaggac gtattctcgc
     1501 tgcagagctt cgacccgaac gcgccggagg ggcagggcat caaccccgtc gcgccgggcc
     1561 aggtgcccgg cctgctcgac ggcgagggtt ttttgaagga gttcgccgac ctctatcgct
     1621 attaccggga caccaagctg cgccggctca aggcgacgga gaacaagctg ttcgcggtgt
     1681 tccaggtcag cgcggcgctc gaggacgtca agatcttcca ctggaacgtg ctgcccaacg
     1741 gatcggtgcg ctacgtcgat aatcgcggcg accgcgagtt cccgcgcccg gcctcgcacg
     1801 acttcgagtg gaccaacacc acgcgcgacg accacgtctc gggcccgcac ccgcacgtca
     1861 acatcaagga cctggtgttc gtcgagacgg tcaacggcga cctgacgatc aaggtcgaga
     1921 ataataccaa ggacgggctc gggctgtacc gcgagccggt cgacgacgcc aaccaggcgc
     1981 tcgacgacgc caagatcctg tacgcgatca tgggcccgct ggtgctgctc aagatcttgc
     2041 cgtaccgcga gctcaagtgg cgctatttta tttacaacac gcgcgt
//
LOCUS       DASWNJ010001863         2232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_317044, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001863 DASWNJ010000000
VERSION     DASWNJ010001863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317044"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..1720)
                     /locus_tag="VGB85_31070"
     CDS             complement(263..1720)
                     /locus_tag="VGB85_31070"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY0138581.1"
                     /translation="MSVILRSAPLFAALALLACERTSGRHDALDTPPPPFVALGPATP
                     LAQLPKITDGAEAAKPETPKDECGRGSGRDPDGKCVPLGLRETEFVQRVQIPAGKFVM
                     GALPGDYNGMLAQEAGVVRWSGQPPRHATSPGFWIDLHEVTRAAYAACVAAKRCTPAT
                     CPVGQVDPATEVAAEVAGVLPQTCVTHTQAAAYCKHAGGRLPNEAEWEYAARGVDARV
                     YPWGNEIEDTIPQAIYPAGHVREDRSYFGILGLGSNVYEWVADVYDMDVGLRPFLGDT
                     QFRDANGPAIAARARFERKIVCGAEAGPECTGPAELPVRHVVKHNNSGHRLAGRDEIP
                     ANYPGFELEGWQTGGTGPKVGFRCVTDLAPGDVPLTVPAPPAQVPLFRVEAGFQIFGG
                     VAEAVTQDEARRFCAGLQVPDLAGNTVGGWRLPTQMELPVVAPSFRGPGPFWVEDGAV
                     IQISETKPLPPDAPWKWHVVPPETPLLARCVRAIQ"
     gene            complement(1732..>2232)
                     /locus_tag="VGB85_31075"
     CDS             complement(1732..>2232)
                     /locus_tag="VGB85_31075"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138582.1"
                     /translation="AVLAASMSTGDSLLHGGSSVFVRDVLLRGLGVRLTERSQTWALR
                     GAVLALGLMGLWATSLAANWSLVDLLLLAYAVPVQFLTITLLGLYWRRANRIAGEAGL
                     CAGLVCVIALFAAGKLAPGLYATLNPLDLQIGVIGLAVNAAVMVLLSLLTPPMAKSHL
                     QRFELS"
BASE COUNT          324 a          817 c          798 g          293 t
ORIGIN      
        1 cgcgagtcga tcaagtactt catcctgccc ccacgacgac cagagaggcg ttacccgcgt
       61 ctcgtcaagc gacccaagag cccttacccc aagaaacgcc gcgcgcagcc ggccctacag
      121 cctaagtgag tggcattggg gaggacccgc gtcgcaggga ccagccgtcc gtcggcccgt
      181 ccccggccca gatagtctcg ccccgcgtcg cccgaacacg cgagcctccg gcgcccgagc
      241 actcgccccc gagcacgcgc ccctactgga tcgcccgcac acagcgagcg agcagcggcg
      301 tctccggcgg cacgacatgc cacttccacg gggcgtcggg cggcagcggc ttggtctccg
      361 agatctggat gaccgcgccg tcctccaccc agaagggtcc tggcccgcgg aagctcgggg
      421 cgaccaccgg cagctccatt tgcgtcggca agcgccagcc gccgaccgta ttgccggcga
      481 ggtcgggcac ttgcaggccc gcgcagaagc ggcgcgcctc gtcttgcgtg actgcctcgg
      541 cgacgccgcc gaagatctgg aagccggcct cgacgcggaa cagcggcacc tgggcgggcg
      601 gggccgggac cgtcagcggg acgtcgcccg gcgccaggtc ggtgacgcag cgaaagccga
      661 ccttgggtcc ggtgccgccg gtttgccagc cctcgagctc gaagccgggg tagttcgcgg
      721 ggatctcgtc gcggcctgcg aggcggtggc cggagttgtt gtgtttgacc acgtggcgca
      781 ctggcagctc ggccgggccg gtgcactcgg gtccggcctc ggcgccgcac acgatcttgc
      841 gctcgaagcg ggcgcgcgcc gcgatcgccg ggccattcgc gtcgcggaac tgggtgtcgc
      901 cgaggaacgg tcgcagcccg acgtccatgt cgtagacgtc ggcgacccac tcgtagacgt
      961 tgctgccgag gccgaggatg ccgaagtagc tcctgtcctc gcggacatgt cccgccggat
     1021 agatcgcctg tgggatcgtg tcctcgatct cgttgcccca cgggtacacg cgcgcgtcga
     1081 cgccgcgggc cgcgtactcc cactcggcct cgttcggcag gcggccgccg gcgtgcttgc
     1141 agtaggccgc cgcttgcgtg tgcgtcacgc aggtctgcgg gagcacgccc gcgacctccg
     1201 ccgcgacctc ggtcgccggg tcgacctgtc ccacgggaca ggtggccggc gtgcagcgtt
     1261 tggccgccac gcacgccgcg taggccgcgc gggtcacctc gtgcaggtcg atccagaagc
     1321 ccggcgaggt cgcgtggcgc ggcggctggc ccgaccagcg cacgaccccg gcctcctgcg
     1381 cgagcatccc gttgtagtcg ccgggcagcg cccccatcac gaacttgccg gcggggatct
     1441 gcacgcgctg cacgaactcc gtctcacgca ggccgagcgg cacgcacttg ccgtcggggt
     1501 cgcgcccgct gccgcggccg cactcgtcct tgggcgtctc cggcttggcc gcctcggcgc
     1561 catcggtgat cttgggcagc tgggcgagcg gggtcgccgg tccgagcgcg acgaacgggg
     1621 gcggcggggt gtcgagcgcg tcgtggcggc cgctcgtgcg ctcgcaagcg agcagggcga
     1681 gcgcggcgaa caggggggcg gatcgaagaa ttacggacat gcggggatcc atcaggacag
     1741 ctcgaagcgc tgcaggtggg actttgccat cggtggggtc agcagggaca gcaggaccat
     1801 gaccgcggcg ttgaccgcga ggccgatgac gccgatctgc aggtcgagcg ggttgagcgt
     1861 cgcgtacagc cctggtgcca gcttgcccgc ggcgaacagc gcgatcacgc acacgagccc
     1921 ggcgcacagg cccgcctcgc cggcgatgcg gttggcccgg cgccagtaca ggccgagcag
     1981 cgtgatcgtc aggaactgca ccggcaccgc gtaggcgagc agcagcaggt cgaccagcga
     2041 ccagttcgcc gccaggctgg tcgcccacag ccccatcagc ccgagcgcca gcacggcccc
     2101 gcgcagcgcc cacgtctggc tgcgctcggt cagccgcacg ccgagcccgc gcagcagcac
     2161 gtcgcgcacg aacaccgatg acccgccgtg cagcagcgag tcgccggtcg acatcgacgc
     2221 cgcgagcacc gc
//
LOCUS       DASWNJ010001864         1545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_703266, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001864 DASWNJ010000000
VERSION     DASWNJ010001864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703266"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VGB85_31080"
     CDS             complement(<1..418)
                     /locus_tag="VGB85_31080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138583.1"
                     /translation="MLRTLPALLLALPACTSPGDDTTTDASTLSTSDMSEGTASTGGS
                     TGDAPTTGDSSSSGGDLPGLTLDAPCTIDAIAPTRLAVITNDFVDPAKLHVVDLATRT
                     LTPDLAPAPSDPALAWGDGKLVVLGRFGINTLDIYDG"
     gene            complement(443..>1545)
                     /locus_tag="VGB85_31085"
     CDS             complement(443..>1545)
                     /locus_tag="VGB85_31085"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0138584.1"
                     /translation="PRVHLGLWRGATLTTIADARQEWTRVDQRTLPSPRGDARRQRIG
                     LGLGIQLDQRLLAGRLHLQPIVRLDLVTSRFAVPAGSGEQDDRGANHLDIGLSPRLGA
                     RLEILRGVSLRLTGGRYFRPPTLVELFGDRGYIVGNEALRPERGTSLDGGVVVDAGSA
                     RTSLYAHAAGFVAWSDRLIQWQQSGPTLRPVNLEGARVSGLETSAALRLLGGDLRLTA
                     NYTLLAPVNLGDDPSQRGQPLPGRPRHELYANCSLGHVFKARSTTLAPRVFYTVEHAA
                     RTFLDPSGRYEVPSRTLHGLGVELRIAERVHLAAELRNLFNLRTTTWHPPIAGAPAIT
                     VPVSDFFFYPLPGLSLWTSLRVDLDLPVRRRA"
BASE COUNT          205 a          436 c          702 g          202 t
ORIGIN      
        1 ggccgtcgta gatgtcgagg gtgttgatgc cgaagcgacc gagcacgacg agcttgccgt
       61 cgccccacgc gagcgcgggg tcactcggcg cgggggcgag gtcgggggtc agcgtgcgtg
      121 tggcgaggtc gacgacgtgc agcttcgcgg gatcgacgaa gtcgttggtg atgacggcga
      181 ggcgggtggg ggcgatcgcg tcgatcgtgc acggagcgtc gagggtgagg ccgggcaggt
      241 cgccgccgct gctcgacgag tcgccggtgg tcggggcgtc gccggtggag ccgccggtcg
      301 aagctgtccc ttcggacatg tcgctcgtgc tcaaggtcga ggcgtcggtg gtggtgtcgt
      361 cgccgggcga ggtgcaggcg ggcagggcga ggaggagggc ggggagggtg cggagcatgg
      421 tcattggggg cggtcggggg ggtcaggcgc ggcggcggac cgggaggtcg aggtcgacgc
      481 gcagggaagt ccacaggctg aggccgggga gcgggtagaa gaagaagtcg gagacgggga
      541 cggtgatcgc gggcgcgccg gcgatcggcg ggtgccaggt cgtggtgcgc aggttgaaga
      601 ggttgcgcag ctcggcggcg aggtggacgc gctcggcgat cctcagctcg acgccgaggc
      661 cgtggagggt gcggctcggg acctcgtagc ggccgctcgg gtcgaggaag gtgcgggcgg
      721 cgtgctcgac ggtgtagaag acgcgcggcg cgagcgtggt ggacctggcc ttgaagacat
      781 gtcccagaga acagttggcg tagagctcgt ggcgcgggcg gccgggcagc ggctggccgc
      841 gctggctcgg gtcgtcgccg aggttgacgg gggcgagcag ggtgtagttg gcggtcaggc
      901 gcaggtcgcc gccgagcagg cgcagcgcgg cgctggtctc gaggccggag acgcgcgcgc
      961 cctcgaggtt gaccgggcgc agggtcgggc cgctctgctg ccactggatc aggcggtcgc
     1021 tccacgcgac aaagccggcc gcgtgggcgt agaggctggt gcgcgcgctg ccggcgtcga
     1081 cgacgacgcc gccgtcgagg ctggtgccgc gctcgggtcg cagggcctcg ttgccgacga
     1141 tgtagccgcg atcgccgaac agctcgacga gggtaggggg ccggaagtag cgcccgccgg
     1201 tcaggcgcag cgagacgccg cgaaggatct ccaggcgggc gccgagccgg gggctaagac
     1261 caatgtcgag gtggttggcg ccgcggtcgt cctgctcgcc gctgccggcc gggacggcga
     1321 agcggctggt gacgaggtcg aggcgcacga tcggctggag gtgcaggcga ccggcgagca
     1381 ggcgctggtc gagctggatc ccgaggccga ggccgatccg ctggcggcgg gcgtcgccgc
     1441 ggggggaggg cagggtgcgc tggtcgacgc gggtccactc ctggcgggcg tcggcgatcg
     1501 tcgtcagggt ggcgccgcgc cataaaccaa ggtgcacgcg cggcg
//
LOCUS       DASWNJ010001865         4119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_527033, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001865 DASWNJ010000000
VERSION     DASWNJ010001865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527033"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VGB85_31090"
     CDS             <1..243
                     /locus_tag="VGB85_31090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138585.1"
                     /translation="LHGPLTPNEPTDAALRDVKEYSASLPVILVDELVVRRNLPTATA
                     QIIERLGSARSKRDSIEGYTGAVDRHHADDVARRAR"
     gene            194..1390
                     /locus_tag="VGB85_31095"
     CDS             194..1390
                     /locus_tag="VGB85_31095"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY0138586.1"
                     /translation="MQLTVITPTTWPAAPAEMSVSTYAEIEECPRRWALGAAEYPDLW
                     SGRGYPPRLQVAALAGSVVHLALEIIIKQLTRAGASSQDHLLVTQVLKELGGYTNVVQ
                     HCVERILKRFADNPRAIPLMEHAQRTLRGQVPTLRMRVQAMLARLHLKAGAAASTPMV
                     ASTPGQLALTNGTYPEIELRATYIGWKGKADLLILADDACEITDFKTGAADEGHTFQV
                     RVYAVLWCLDDQLNPSGRVASRLVLAYDSHDVDVPPPSAQEIDDLGRELLGRRQAAEI
                     ALAARPPAARPSAETCRYCSVRHLCDAYWAGTAQAVSDDGRFGDVELKIVSRHGPTSW
                     DAVVVRARNLPTKSPALLRLQQPADFNVGTHVRVLDGALARDPEDADTPIIVTLGAFS
                     ETYRVE"
     gene            complement(1804..2847)
                     /locus_tag="VGB85_31100"
     CDS             complement(1804..2847)
                     /locus_tag="VGB85_31100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138587.1"
                     /translation="MAHVPYYRIDTRPIIPMQLVLLDMNIFVNISERDASEEQLVRTL
                     RAHGLTAVRTVELIQEAARLRDADARLARVRVIHRVTKHIFDPRLLADTHELRDELRI
                     HRRSWLRLVPARAKIVQLQRKWRQWLQEEPKPDQIAALAALHQEEIEKNKTLHKELRS
                     AATAPGAYIEPMIECSDPHLREAVLAKAPPRALFSQIGDDPALFAQWMRYHAREWLFE
                     EYRAAMFDREFRLSDLADYLEPYLGRPAFRSVLDWARFCFVDAAIENCPRKLLAAGVV
                     LGQQRRKFDAGNVGDVNHTSYLRDVDVFLTSDKNFAAALGRLPPALVGAAVGVVQPNH
                     ADLEDVVRQALRC"
     gene            3216..>4119
                     /locus_tag="VGB85_31105"
     CDS             3216..>4119
                     /locus_tag="VGB85_31105"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0138588.1"
                     /translation="MCTERLDDANTTWEQTDASDEVDGDEVGEDTSDEVGEDADQGDS
                     LLLDEDEGAPNLVPEGHPQRVLAGRIEILDKIGEGGMGAVWRGLHLKLERVVAVKILD
                     ETLQLRPDGRERFIREARALALLSHRNVVTIYDCDELSDGKLYLCMELLAGETLREII
                     KRRQPLDPLEVIDAGMQACDAVQAAHDRGILHRDLTPSNIIRLHDDAKTIKVIDWGLC
                     KYLDLFYVRSTQKYAAPPGSRLVTPLGCRFGTPEYMAPEMFLRENPDPPSFCTDVYAL
                     AVVLYELLTGRHPFAPGQRQHPRPV"
BASE COUNT          738 a         1331 c         1341 g          709 t
ORIGIN      
        1 ctgcatggtc cgttgacgcc caacgagccg accgacgccg cgctccgtga cgtcaaggag
       61 tactccgcgt cgctgccggt catcctcgtc gacgaactgg tagtgcgtcg aaacctcccg
      121 accgccaccg cgcaaatcat cgagagactc ggctcagcga ggtccaaacg tgacagcatc
      181 gaaggataca caggtgcagt tgaccgtcat cacgccgacg acgtggcccg ccgcgcccgc
      241 tgagatgagc gtgtcgacgt acgcggagat cgaggagtgt ccgcgccggt gggccctcgg
      301 cgccgccgag tacccggacc tgtggagcgg gcgtggttat ccaccgaggc tccaagtggc
      361 ggcgctagcg gggagcgtcg tccacctcgc gctcgagatc atcatcaagc aactcacgcg
      421 cgctggtgcg tcgtcgcagg accacctgct agtgacgcag gtgctgaagg agttgggcgg
      481 gtacacaaac gtcgtgcaac attgcgtcga gcgcatcctc aagcgcttcg ccgacaaccc
      541 gcgcgcgatc ccgctaatgg agcacgcgca gaggacgttg cgtggccaag tcccgacgct
      601 tcgcatgcgc gtccaagcga tgctggcccg tctccatctc aaagcggggg ccgctgcgtc
      661 aacgccgatg gtggcgtcaa cgccgggaca actcgcgctc acgaacggca cgtatcccga
      721 gattgagctc cgcgcgacgt atattgggtg gaagggtaag gccgatctgc ttatcctcgc
      781 ggacgacgcg tgcgagatca ccgacttcaa gacgggcgcc gccgatgagg ggcacacctt
      841 tcaggtgcgg gtatacgctg tactgtggtg cctcgacgac cagctcaacc catcgggccg
      901 tgtcgctagc cggctcgtgc tcgcgtacga cagccatgac gtcgacgtgc cgccgccgag
      961 cgcacaggag atcgatgacc tcggtcgaga gcttctgggg cgtcggcaag cggccgagat
     1021 agcgttggct gcgcgcccac ctgccgcgcg ccccagcgcg gagacttgtc gctactgcag
     1081 cgtgcgccac ctctgcgatg cctattgggc cgggaccgcg caagcggtct cggacgatgg
     1141 ccgcttcggg gacgtcgaac tgaaaatagt cagtcgacac ggcccaacga gttgggacgc
     1201 ggtcgtcgtg cgtgctcgca atctgccgac gaagtcgccc gcattgttgc ggctccaaca
     1261 gcccgccgac ttcaatgtgg gcacccacgt gcgcgtactc gacggtgcct tggcacgaga
     1321 cccagaggac gccgacacac cgatcatcgt cacgctcggc gcgttcagcg agacctatcg
     1381 cgtcgagtag tccccggtca cttacagaac gtcctggcat atcgcggggt gcgcccgcgg
     1441 accttctccg acaacggccc gaagtccatt gccggcgacc tccgcggctt catcaagctc
     1501 gccagcgcgg gggcaggcgg ggtgctggac ctgccggaaa ctgaccgctg gggaaatcgc
     1561 cgacgtgtcc ggcgactgcg caggcgacct catgcagaat cggccaccgc gaaatcgccc
     1621 tgtgggctca accaactaga cgtacctctg cccgtcgctg cgcacgactg gccacgttgg
     1681 gaaaatggcc gcggctaccg cgcgaccctg aaatactagc ccgcggcgta gctcgccgcc
     1741 gcggatgcag ccgcgggcgc ccacttcagg atgcgtcatc cacgagaggg ctggaacacc
     1801 tcgtcaacac cgaagcgcct ggcgcacgac gtcctcgagg tccgcgtgat tgggctgaac
     1861 gaccccgacc gccgcaccga ccagcgcggg gggtaggcgt ccgagcgcgg cggcgaaatt
     1921 cttgtccgac gtcagaaaca cgtccacgtc gcggaggtac gacgtgtgat tcacgtcacc
     1981 gacattacca gcgtcgaatt tccttcgttg ttggccgagg accacaccgg cagcgaggag
     2041 cttgcgcggg cagttctcga tcgcggcgtc tacgaagcaa aaacgtgccc agtcgagtac
     2101 gcttcgaaaa gcgggtcgac cgagatacgg ttcgagatag tccgcgagat cactcagccg
     2161 aaactcgcgg tcgaacatgg cggcccgata ctcttcgaac aaccattcgc gagcatgata
     2221 cctcatccac tgtgcgaaca gggccgggtc atcgccgatc tgcgagaata gtgcccgcgg
     2281 gggagccttg gccagcaccg cttcacggag atgggggtcg gaacattcga tcatgggttc
     2341 gatataggcg ccgggagcag tggccgcact gcgcaattct ttgtgcagcg tcttgttctt
     2401 ctcgatttcc tcctgatgaa gcgccgcgag cgccgcgatc tgatcaggct tcggctcctc
     2461 ctgcagccat tgccgccact tacgttggag ctggacgatc tttgccctgg ccggaacgag
     2521 cctaagccag gatcgacgat gtatccggag ttcgtcgcgc agttcgtggg tgtccgccag
     2581 caggcgcgga tcgaatatat gcttcgttac cctgtggatc acccggacac gcgcgagccg
     2641 ggcgtcggcg tcacgcagtc gcgctgcctc ctgaatcagc tcgacggtgc gtaccgcggt
     2701 gagaccatgc gccctcagcg ttcggaccag ttgctcctcg cttgcgtccc gttcgctgat
     2761 attgacgaat atgttcatgt ccagaagtac gagttgcatg gggatgatcg gtcgagtgtc
     2821 gatgcgataa tagggcacgt gcgccatctg caccaggtac tgctggcatg gccctccccc
     2881 gcatgcgatc gcgggtgtcc gggcgagcgg ccggcactga cccggtccga actcgagccc
     2941 cggcgtttgg gtaccgctga cccggcgttt gggtaccact gatccggagg atgtggtcgc
     3001 gggctggtgc ttaatgtcgg tggcgatctg tgcgcggggg ccgttgtgat cgatgagcgt
     3061 tgcaagcgaa agttggtcaa ctgttagcat catgtgtagg tgtgcgccta cacgggctag
     3121 atgccccatc agcggatgtg ctcgattacg cgctctagcg tcttacgcaa ggttgaggcc
     3181 tggtgcgtgg tactgtcgcc aggtcggagg gcgacatgtg cacggagcgg ctggacgacg
     3241 cgaatacgac ttgggagcag accgatgcca gcgacgaggt cgacggcgac gaggtcggcg
     3301 aggacaccag cgacgaggtc ggcgaggatg ccgatcaagg cgacagcctc ctgctcgacg
     3361 aggacgaggg cgccccgaac ctcgtccccg aggggcatcc gcagcgcgtc ctcgccggtc
     3421 ggattgagat cctcgacaag atcggcgagg gcggcatggg ggccgtgtgg cgcgggctac
     3481 atctcaagct cgagcgcgtc gtcgcggtta agatcctgga cgagactctc cagctccgcc
     3541 cggacggccg cgaaaggttt atccgggagg cccgcgcgct ggcgctgctc tcccaccgca
     3601 acgtggtcac gatctacgac tgcgacgagc tctcggacgg caagctctac ctgtgcatgg
     3661 agttgctcgc gggagagacg ctgcgcgaga tcatcaagcg tcgccagcct ctcgatccgc
     3721 ttgaggtgat cgacgcgggc atgcaggcct gcgatgccgt ccaggcggcg cacgaccgcg
     3781 gcatcctgca ccgcgacctg acgccgtcga acatcatccg cctgcatgat gacgcgaaga
     3841 cgatcaaggt tatcgactgg ggcctctgca agtaccttga tcttttctac gtgcgctcga
     3901 cgcagaagta cgcggccccg ccgggatcgc ggctcgtgac gccgctcggc tgccggttcg
     3961 gcacgcccga gtacatggcg ccggagatgt tcctccgcga aaaccccgac cccccgagct
     4021 tctgcaccga tgtgtacgcg ctcgcggtcg tgctttacga gttgctcacc gggcggcacc
     4081 cgttcgcacc cggccaacgc cagcacccgc ggccggtcc
//
LOCUS       DASWNJ010001866         4319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_527037, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001866 DASWNJ010000000
VERSION     DASWNJ010001866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527037"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..997
                     /locus_tag="VGB85_31110"
     CDS             167..997
                     /locus_tag="VGB85_31110"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0138589.1"
                     /translation="MKILVTGATGKVGSRLAHRLASRGHHVRALVRDPRRADALRKHD
                     IEIVQGDLLDSTSLAAAVRDIDAVVHCAAFFRGATPEQAHAVNDVGTQHLASAARAAS
                     VQRFVFTSTGLVYGSTGSRAAGEDDPCTPVPGYPASKLAAERALFAVDGIDVRVLRLP
                     FVYGDGDPHIAEVVPMMRGASPTQRMSIAHHVDIAQAVARLLETASPSHRIYNVADDE
                     APDLVTLFASVGAPPPDGSAAEAGRTFEIVLDVRRIRDDLGYRPEYPRLHDAIVAGKA
                     "
     gene            1255..1683
                     /locus_tag="VGB85_31115"
     CDS             1255..1683
                     /locus_tag="VGB85_31115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138590.1"
                     /translation="MFRATWLSSFVIAVVVGNLTQCEGGGGQDDCIAGSEACECLQGE
                     CFSDLECVRGMCVSLGGATSQGPTDGGSDASGNVTDSPTGGPTTASTTASTTASTTAS
                     TSGPTGDPTGSPTGDPTGDPTSDPGTTSGPGTDTDPDPSD"
     gene            1693..2643
                     /locus_tag="VGB85_31120"
     CDS             1693..2643
                     /locus_tag="VGB85_31120"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEY0138591.1"
                     /translation="MPGSCKQLQLDKIPANVMLVVDRSGSIAKPWDQGQNKWTSVHET
                     LTTAIANNQAGIKFGLKMFPSANATSDYSEAACVTSATLEVPLALANSANISAALPPA
                     FGPAAGGSPTAKGLTAAVKALQGAPASEGRTIVFISDGPFNCMAGAQDNSSLFEILDE
                     DPHSIVADAWTQDGISTIVVGVGVSGDTSGVVKDGNPDNTNAYSHFQTLAVEGGQPRA
                     GEEKFYAAYTEAELAGFMVEAIDKAETCTLALGLTPIDPESTTIKVAGTSYPYMPGLN
                     CATGNGWVYSDGNNTRLALCGTACDALKSVGSAEVDVDCE"
     gene            2824..4146
                     /locus_tag="VGB85_31125"
     CDS             2824..4146
                     /locus_tag="VGB85_31125"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0138592.1"
                     /translation="MSTVGLEPVDTTTDPCVDLYRHACGRWLAEHPRPLASPVWARSF
                     SAAEAQAQARVLALLDELAGAKDGVHARLGAYWRACIDEDGRIAAGYTAIEPLLRAID
                     DARPRDLGSVLGRLHAHGIPALFHVRPTQDPAGRTLRITLGGTGLGTPQMYAPSAPRL
                     TAYRQHVAEMLRLAGLADAPRRAAAVVAFEARLAALQSTSAELVAEQSRPWPARPVAT
                     LRQRAPSIDWERYFAAQGPQLPGRIIVPPSHMRDLDALLRRTDIGVLRDYLRWQLLHA
                     LATSLPPVFEIEHNRMYDPAAAVAGRTASCVRHVDDGFGPTLGAHTSSATSRPATASG
                     FRSWPGSCARPCAPRSSAPPGSTRPHAPRCSPTSIPLRPRSPSRTCGPTRWRACPRRP
                     TFSPPRWRYAWPGSPPVTPRPPAAPGRPWPPPRSTGRCGATSSSCSPE"
     gene            4110..>4319
                     /locus_tag="VGB85_31130"
     CDS             4110..>4319
                     /locus_tag="VGB85_31130"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HEY0138593.1"
                     /translation="MRGDKLELFAGMIQPPFYTADMPDPVMLGAVGQILAHEFGHILD
                     PETLAAVPAWRPTPITAAAYAARVQC"
BASE COUNT          662 a         1648 c         1408 g          601 t
ORIGIN      
        1 gacgaccgcc gccgtcgacg cgccgcccgg gtcataggac tggcgatcgg cggcgctcgc
       61 gtgcatgccc gacaggatga ggtcaggcgc tcgcccgcac aagcgggccg cgacgttgtc
      121 cggtttctcc aatctctcgg cagcgcggct cgctacggtc ggcgccatga agatcctcgt
      181 cacaggagcg accggaaagg tcggaagccg tctcgcccat cgcctcgcca gccgcggtca
      241 tcacgtgcgc gcactcgtgc gcgatccgag gcgcgccgat gcgctgcgca agcacgacat
      301 cgagatcgtg caaggcgacc tgctcgacag cacctcactc gcggccgcgg tgcgcgacat
      361 cgacgcggtc gtccactgcg ccgcgttctt ccgcggcgcg acgccggagc aggcccacgc
      421 cgtcaacgac gtcggcacgc agcacctcgc gtctgcggca cgcgccgcat cggtgcagcg
      481 cttcgtgttc acgagcaccg ggttggtgta cggctcgacg ggcagccgcg ccgcaggcga
      541 ggacgacccg tgcacaccgg taccgggata cccggcgagc aagctcgcgg cagagcgcgc
      601 cctcttcgcg gtcgacggca tcgacgtccg cgtgctccgc ctccccttcg tgtacggcga
      661 cggcgatccg cacatcgcgg aggtcgtccc gatgatgcgc ggcgcctcgc cgacgcagcg
      721 catgtcgatc gctcaccacg tcgacatcgc ccaggcggtc gcgcgcctgc tcgagacggc
      781 gtcgccgtcg caccgcatct acaacgtcgc cgacgacgag gcgcccgacc tggtgacgct
      841 ctttgcatcg gtcggcgcgc cgccgccgga tggctccgcc gccgaggccg ggcgcacgtt
      901 cgagatcgtg ctcgatgtcc gccggatccg cgacgacctc ggctatcgtc cggagtaccc
      961 gcgcctccac gacgcgatcg tcgccggcaa agcatgacgg caggcgcgtc cgatgtgggc
     1021 gagcaatccc cgccaatgtt caccgttctt tgcaccggcc ccatcctggc ccggcgctcg
     1081 cgatacttgc caccctgagc gagatcctcg gcccacaccg ccgtccgcgc aataaacacg
     1141 cgcccgtcgt ctcctccagc gcttgtcgcc gccgcggctc cctgttagct tggtcgaaca
     1201 ttcggcgcga acgtgcgctc gaacgatggg atcgttgact tcggagatcg tgaaatgttt
     1261 cgtgcaacct ggctgagctc gttcgtgatc gcggtggtgg tcggcaactt gacgcaatgt
     1321 gagggcggcg gcggccagga cgactgcatc gccggctccg aggcttgcga gtgcctgcag
     1381 ggcgagtgct tctccgacct cgagtgtgtc cgggggatgt gcgtcagcct cggcggcgcc
     1441 acctcgcagg gcccgaccga cggcggcagc gatgccagcg gcaacgtgac cgacagcccg
     1501 accggcggcc cgaccaccgc ctcgaccacc gcctcgacca ccgcctcgac caccgcctcg
     1561 accagcggcc cgacggggga cccgaccggc agcccgacgg gagacccgac cggagatccg
     1621 acgagcgacc cgggcaccac cagcggcccc ggcacggaca cggacccgga tccgagcgat
     1681 tagatcacca cgatgccagg cagctgcaag cagctccagc tcgacaagat ccctgccaat
     1741 gtgatgctcg tggtcgaccg cagcggatcg atcgccaagc cgtgggacca gggccagaac
     1801 aagtggacca gcgtgcacga gacgctgacc acggcaatcg ccaacaatca ggcgggcatc
     1861 aagttcggcc tgaagatgtt cccctcggcc aacgccacca gcgactactc cgaggcggcc
     1921 tgcgtcacca gtgccacact cgaggtgccg ctggccctcg ccaattccgc gaacatctcg
     1981 gccgcgctgc cgcccgcgtt cggacccgcc gcgggcggct cgccgacggc gaagggcctg
     2041 accgcggcgg tcaaagccct tcagggcgcg cccgcgagcg agggccggac gatcgtcttc
     2101 atcagcgacg gcccgttcaa ctgcatggcc ggggcccagg acaatagctc gctgttcgag
     2161 atcctcgacg aggaccccca cagcatcgtc gccgacgcat ggacacagga cgggatctcc
     2221 acgatcgtcg tcggcgtcgg tgtctccggg gataccagcg gcgtggtcaa ggacggtaac
     2281 cccgacaata ccaacgccta ttcccacttc cagaccctcg cggtcgaggg cgggcagccg
     2341 cgcgccgggg aggagaaatt ctacgccgca tacaccgagg ccgagctcgc gggattcatg
     2401 gtcgaggcga tcgacaaggc cgagacctgc acgctcgccc tcggtctcac gccgatcgac
     2461 cccgagagca cgaccataaa ggtcgccggc accagctacc cgtatatgcc cggcctgaat
     2521 tgcgccaccg gtaacggctg ggtctacagc gacggcaaca acacccggct cgcgctgtgc
     2581 ggcaccgcct gcgacgccct caagtcggtc ggcagcgccg aggtcgacgt cgactgcgaa
     2641 taagcgcgcg agcccgtggg tcggcgtgac ttcgaggccg gcgcggtcca tcctggggat
     2701 cccttgagcg cacgcgcggc cctcctcgtc ctgttcgtgt cgggctgccg gcctcccgcg
     2761 acatccgcga catccgcgac atccgccccg gtcgagccag gatccgcgac cccggcagct
     2821 tccgtgtcga ccgtcggcct cgagcccgtc gataccacca ccgacccctg cgtcgatctt
     2881 tatcgacacg cgtgcggtcg ctggctcgcc gagcatccgc ggccgctcgc gtcgccggtg
     2941 tgggcgcgca gcttcagcgc tgccgaggcg caggcgcagg cgcgggtgct cgcgctgctc
     3001 gacgagctcg cgggtgcaaa ggacggcgta cacgccaggc tcggcgccta ctggcgggcg
     3061 tgcatcgacg aggatggccg gatcgccgcc ggctacaccg cgatcgagcc gctgctgcgc
     3121 gcgatcgacg acgcccgccc gcgcgacctc ggctccgtgc tgggaagact ccacgcgcac
     3181 gggatcccgg cgctgtttca cgtgagaccg acgcaggacc ccgccggccg gacgctgagg
     3241 atcaccctcg gcggcactgg cctgggcacg ccgcagatgt acgcgccgtc cgccccgcgg
     3301 ctgaccgcct atcgccagca cgtcgccgag atgctgcgac tcgccggcct cgcagacgca
     3361 ccgcgccgcg ccgccgccgt ggtcgccttc gaggccaggc tcgccgcact gcagtcgacc
     3421 tccgccgagc tggtcgccga acagagccgg ccgtggcccg cgcgacctgt ggccacgttg
     3481 cgtcagcggg ccccgtcgat cgactgggag cgttacttcg ccgcgcaggg tccacagctc
     3541 cccgggcgta tcatcgtccc cccctcgcac atgcgcgacc tcgacgcgct gctgcgccgc
     3601 accgatatcg gcgtactccg cgattatctg cgctggcagc tgttgcacgc gctcgccacg
     3661 agcttgcccc cggtgttcga gatcgagcac aatcgcatgt acgaccccgc ggccgcggtc
     3721 gccggacgca cggcgagctg cgtgcgacac gtcgatgacg gcttcggccc gactctgggc
     3781 gcacatacat cgagcgccac gtcacgcccg gcgaccgcgt ccggcttcag gtcatggccg
     3841 ggaagctgcg cgcggccctg cgcgccgagg tcgagcgcgc cgcctggatc gacccgcccg
     3901 cacgcgccgc ggtgctcgcc cacatcgata ccattgcgtc cgagatcgcc gagccggacc
     3961 tgcggcccga cccgctggcg agcatgcccg cgacgaccga ctttctcgcc acctcgctgg
     4021 cgctacgcct ggcccgggtc gccgccggtg acgccgcggc caccagcggc gccgggtcgg
     4081 ccctggccgc cacctcggtc aacggggcga tgcggggcga caagctcgag ctgttcgccg
     4141 gaatgatcca gcccccgttc tacaccgccg acatgcccga tccggtcatg ctcggcgccg
     4201 tgggtcagat cctcgcgcat gaattcggcc acatcctcga ccccgagacc ctcgcggcgg
     4261 tccccgcctg gaggcccacc ccgatcaccg cagcagccta cgccgcgcgc gtccagtgt
//
LOCUS       DASWNJ010001867         2512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_492499, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001867 DASWNJ010000000
VERSION     DASWNJ010001867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492499"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1885
                     /locus_tag="VGB85_31135"
     CDS             <1..1885
                     /locus_tag="VGB85_31135"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY0138594.1"
                     /translation="TIDAPDGCGRYLGYVIEGVRVGPSPLWLRVRMHRLGLKPISNVV
                     DITNLVLMEYGQPLHAFDRDRLAEGRVVVRRARHDEPLRTLDGVDRVLDPRDLVIADA
                     HDAQALAGVMGGAASMVHAGTTAILLEAAWFASAGIRASARRHGLGTDASYRYERGVD
                     HGVGLEHAALRALGLLEQLTGGRCVAQAEATGQRPPRPRITLRPARAEALLGVAVPTD
                     EARAILRGIEVEVDERDPASWSCLAPTHRPDLQREVDLIDELLRHHGLRHVPAQASVP
                     REARAAASADPMTVRSDRVADGLREAGLQEIVSLAFVAEDKLRHFVAEVPEDRHVRVA
                     NPMRGSGVMRTHLLPGLLDALAHNVARHGRAVRLFEIGRIYAWPEGQLDPNDFPAGTR
                     AVDVQLPVERTRAGVLLYAGTKGHADPRAVTGAIAHVLARLGHRLHLSQGTGARGYLH
                     PGVQVQLAIPTASGVDAAVGFAGELHPELVAAWGLPDGARVIYGELDLEALPDPAVAV
                     AREIPRFPATSRDLSLEIPLAVPAAEVVAALRDAARAQTGSGEDPARLQESGTGEGGV
                     EVLEDYRGAGVPDAHRALLLRLHYAAGARSVTDAEVQTLHAAIVEQACARLRGLAPSV
                     RPR"
     gene            1940..2221
                     /locus_tag="VGB85_31140"
     CDS             1940..2221
                     /locus_tag="VGB85_31140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEY0138595.1"
                     /translation="MTKADIIDKVCEEVGGFSKKEAADVVEAVFELMKNTLAEGEKIK
                     ISGFGNFVVRTKQPRPGRNPQTGEEIVIEARRVLTFKPSNVLKTALNDE"
     gene            2258..>2512
                     /locus_tag="VGB85_31145"
     CDS             2258..>2512
                     /locus_tag="VGB85_31145"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY0138596.1"
                     /translation="MNQPSEEDELFIAEIGPAYAAPEDAPEPEPAPPRARTAAKKARK
                     RSATAEPTVDLGSEKLYFKIGEVAQIVGVPAYVLRYWETEF"
BASE COUNT          351 a          923 c          912 g          326 t
ORIGIN      
        1 cacgatcgac gcccccgacg gctgcggtcg ttacctcggc tacgtgatcg agggcgtgcg
       61 cgtcgggccc tcgccgctgt ggctgcgcgt ccgcatgcat cgcctgggcc tcaagccgat
      121 ctccaacgtc gtcgacatca ccaacctcgt gttgatggag tatggccagc cgctgcacgc
      181 cttcgaccgc gaccggctcg cggagggcag ggtggtcgtt cgccgcgcgc ggcacgacga
      241 gcccctgcgc acgctcgacg gcgtcgaccg ggtgctcgac ccgcgcgacc tcgtcatcgc
      301 cgacgcccat gacgcccagg cgctcgcggg cgtgatgggc ggcgcggcct cgatggtcca
      361 cgccggcacc accgcgatcc tgctcgaggc ggcgtggttc gcctccgcgg ggatccgcgc
      421 gagcgcgcga cggcacgggc tcggcaccga cgcctcgtat cgctacgagc gtggcgtcga
      481 ccacggcgtc ggcctcgagc acgcggccct gcgcgcgctc ggtctgctcg agcagctgac
      541 cggtgggcgc tgcgtcgcgc aggccgaggc gaccggccag cgcccgccgc ggccgcggat
      601 cacgttacgt ccggcgcgcg ccgaggcctt gctgggcgtc gccgtgccga ccgacgaggc
      661 ccgggcgatc ctccgcggga tcgaggtcga ggtcgacgag cgcgacccgg ccagctggtc
      721 gtgcctggcc ccgacgcacc gccccgacct ccagcgcgag gtcgacttga ttgatgagct
      781 gctgcgccac cacggccttc ggcacgtgcc ggcgcaggcg agcgtgccgc gcgaggcgag
      841 ggccgccgcg tcggccgacc cgatgaccgt gcgcagcgat cgcgtcgccg acggtctgcg
      901 cgaggccggc ctgcaggaga tcgtgtcgct ggccttcgtc gccgaggaca agctgcgtca
      961 cttcgtggcc gaggtcccgg aggaccgcca cgtccgcgtc gccaacccga tgcgcgggtc
     1021 cggcgtcatg cgcacgcacc tgctgcccgg cctgctcgat gcgctggccc acaacgtcgc
     1081 ccgtcacggg cgggccgtgc ggctgttcga gatcggccgg atctacgcct ggcccgaagg
     1141 acagctcgac ccgaacgact tcccagcggg cacgcgggcc gtcgacgtgc agctgccggt
     1201 cgagcgcacg cgggccggcg tgctgctgta cgccggcacg aaggggcacg ccgatccgcg
     1261 ggcggtcacc ggcgcgatcg cccacgtgct cgcgcgcctc gggcatcgcc tgcacctgtc
     1321 ccaggggaca ggtgcgcgcg gctacctgca cccgggcgtg caggtgcagc tggcgatccc
     1381 gacagcgtcc ggcgtcgatg cggcggtcgg cttcgccggc gagctgcacc ccgagctcgt
     1441 cgccgcgtgg ggtctgcccg atggcgcgcg cgtcatctac ggcgagctcg acctcgaggc
     1501 gctgcccgac cccgcggtcg cggtggcgcg cgagatcccg cgcttccccg cgaccagccg
     1561 cgacctgtcg ctcgagatcc cgctcgccgt gccggcggcc gaggtcgtcg cggcgctgcg
     1621 agacgccgcg cgcgcgcaga ccggcagcgg cgaggaccct gcgcgcctcc aggagagcgg
     1681 gaccggggag ggtggggtcg aggtcctcga ggactaccgc ggcgcgggcg tgccggacgc
     1741 tcaccgcgcg ctgctgttgc gcctgcacta cgcggccgga gcccgctcgg tcaccgacgc
     1801 ggaggtccag acgctgcacg ccgcgatcgt cgagcaggcc tgcgcccgcc tgcgggggct
     1861 cgccccgagc gtgcgccccc gctagggtgc cggcatcact tgcatcccat ggcgatctaa
     1921 gggaaagatc gcggctgcga tgaccaaggc cgacatcata gacaaggtct gcgaggaggt
     1981 cggcgggttc tccaagaagg aggccgccga cgtcgtcgag gcggtgttcg agctgatgaa
     2041 gaacaccctc gccgagggtg agaagatcaa gatctccggc tttggtaact tcgtcgtgcg
     2101 caccaagcag ccgcgccccg gtcgcaaccc gcagaccggc gaggagatcg tcatcgaggc
     2161 ccgccgcgtg ctcaccttca agccgagcaa cgtcctcaag acggcgctca atgacgagta
     2221 gagtcgagtc gtagcgagca gagcgagtcg tgagtcgatg aaccaaccca gcgaagagga
     2281 cgagctgttc atcgccgaga tcggcccggc atacgcggcc cccgaggacg cgccggagcc
     2341 cgagcccgcg ccgccgcgcg ccaggaccgc ggccaagaag gcccgcaagc gcagcgccac
     2401 ggccgagccg accgtcgacc tcggctcgga gaagctgtac ttcaagatcg gcgaggtcgc
     2461 gcagatcgtc ggcgtgccgg cgtacgtgct gcgctactgg gagaccgagt tc
//
LOCUS       DASWNJ010001868         1753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_598178, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001868 DASWNJ010000000
VERSION     DASWNJ010001868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598178"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..786
                     /locus_tag="VGB85_31150"
     CDS             196..786
                     /locus_tag="VGB85_31150"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEY0138597.1"
                     /translation="MAEARAMLAEQREPAAPVPGRPPAQLSPGDRVTLPALGSSGEVV
                     SVKGDKVVVQLGTVRTTVSLADVRSNKAPEVGTTRVKPTTGAPLRQWDKHQKESDETA
                     KHFGADPVPVDFGVDNSVDVRGERAEDALRLVDKMLDDAVRRDQEVVAVIHGHGTGAL
                     KKAVREHLGQLPFVRKTRPGLPKEGGEGVTIAWVGV"
     gene            798..>1753
                     /locus_tag="VGB85_31155"
     CDS             798..>1753
                     /locus_tag="VGB85_31155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138598.1"
                     /translation="MSRSAGPRIVGVALALAASPVLAAPAPLQAGDLAGENRSLLRDV
                     DRTRTNVAGEADAAAVVANPANMSFLKAIGGVIEGSWTRASAQRRGSGAGAYLAIPLS
                     LRLFGRSVADNFLALGVGYQHLRPVPGTRLDGYPRPIDDRQAADMPYHKLSLSLAVPL
                     MRWVPGLSLGLSYSRLWSVANRFADDLDQFDLALAYRPHRVVALGVVARAINTPHMGT
                     WLGDAGGGAWPILRQAAPFELDPEIAVRPIHGTRALELAVGARIAPLTVFDRRFMLPT
                     ISPRVRIEGGGRGVRGFVEAERYWSSPESFIGSNVNGLRVMAG"
BASE COUNT          243 a          591 c          701 g          218 t
ORIGIN      
        1 aaggaggtcg cgcgtcgcga ggccgccgcg ctgcagcaga gcaagagccg ctagcaaaag
       61 gcctacgagg ccgcggcggg cgagctgcgg gcgctgcacg gcgagctggc cgagcagcgc
      121 aaggcgctgc gcaaggccca gcgcgacggc gagctgcctg cggtcgcggc gacggcggag
      181 cagctcggcg gcagggtggc cgaggcgcgg gcgatgctgg cggagcagcg cgagccggcg
      241 gcgccggtgc ctggacggcc gccggcgcag ctgagcccgg gggacagggt cacgttgccg
      301 gcgctcggta gcagcggcga ggtggtgtcg gtcaagggtg acaaggtcgt cgtgcagctc
      361 ggcacggtgc gcacgacggt gtcgctcgcc gacgttcgca gcaacaaggc gcccgaggtc
      421 ggcacgacgc gcgtcaagcc gacgaccggc gcgccgctgc ggcagtggga caagcatcag
      481 aaggagagcg acgagacggc gaagcacttc ggggccgacc cggtgccggt cgacttcggg
      541 gtcgacaaca gcgtcgacgt ccgcggcgag cgggccgagg acgcgctgcg tttggtcgac
      601 aagatgctcg acgacgcggt gcgccgggac caggaggtcg tcgcggtgat ccacgggcac
      661 ggcaccggcg cgctcaagaa ggcggtgcgc gagcacctcg ggcagcttcc gttcgtccgc
      721 aagacccggc ccgggctgcc caaggagggc ggcgaagggg tgacgatcgc gtgggtcggg
      781 gtgtaaggtc gagggacatg tcccgaagcg caggtccgcg gatcgtcgga gtggcactcg
      841 cgctggcggc gagccccgtg ctcgccgcac cggccccgct gcaggcgggc gacctcgcgg
      901 gcgagaaccg ctcgctgctg cgcgacgtcg accgcacgcg caccaacgtc gccggtgagg
      961 ccgacgcggc ggcggtggtc gccaacccgg ccaacatgag cttcctgaag gcgatcggcg
     1021 gggtgatcga gggctcgtgg acgcgggcga gcgcgcagcg ccgggggtcc ggcgccgggg
     1081 cgtacttagc gatcccgctg tcgctgcggc tgttcgggcg cagcgtcgcc gataacttcc
     1141 tggcgctcgg ggtcggctat cagcacctgc ggccggtacc gggcacgcgg ctcgacggct
     1201 acccgcggcc gatcgacgac cggcaggcgg cggacatgcc gtatcacaag ctgtcgctgt
     1261 cgctcgcggt gccgctgatg cgctgggtgc ccgggctgtc gctgggcctg tcgtacagcc
     1321 gcctgtggtc ggtcgccaac cgcttcgcgg acgacctcga tcagttcgac ctggcgctcg
     1381 cgtaccgccc gcaccgggtc gtcgcgctcg gcgtggtcgc acgggcgatc aacacgccgc
     1441 acatgggcac gtggctcggc gacgccgggg gcggagcgtg gccgatcctc cgccaggcgg
     1501 cgcccttcga gctcgacccg gagatcgcgg tgcgaccgat ccacggcacg cgggcgctcg
     1561 agctggcggt cggcgcccgc atcgcgccgc tgacggtgtt cgatcgccgg ttcatgctgc
     1621 cgaccatctc gccgcgcgtg cgcatcgagg gcggcggccg gggcgtgcgt ggcttcgtcg
     1681 aggccgagcg ctactggagc tcccccgaat cgttcatcgg cagcaacgtc aacggcctgc
     1741 gcgtgatggc cgg
//
LOCUS       DASWNJ010001869         2202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_492521, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001869 DASWNJ010000000
VERSION     DASWNJ010001869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492521"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /gene="ftsZ"
                     /locus_tag="VGB85_31160"
     CDS             complement(<1..1248)
                     /gene="ftsZ"
                     /locus_tag="VGB85_31160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY0138599.1"
                     /translation="MAHFELEDAVQDQAKIKVIGVGGAGGNAVNTMIQNKVEGVEFYV
                     ANTDVQALERSLAPTVIQLGRDITRGLGAGANPEKGREAALESIAEIAEALKGADMVF
                     VTAGMGGGTGTGAAPIIAQIAREMGALTVGVVTKPFRFEGKRRLRFAEEGIERLEKAV
                     DTLITIPNDRLLQVTSANTSLLDSFRLADEVLQHATQGVSDLITVPGIINVDFADVRT
                     IMSGQGRALMGMGIGHDEGRALQAAQQAISSPLLEDVTIHGAQGILINITAGPDLKLH
                     EVEEAASLIQEAAHEDCNIIFGAVIDPNMGDALRLTVIATGFDRHAPAEDELEQVIRA
                     HSTGAVSRGRRQSQVQVPGMPGPGLLGNNLGNNPLLGGGNQSASASHTHGHMPVQGHG
                     AAAHMPLGTAAVPARQPLAQDRYA"
     gene            complement(1786..>2202)
                     /locus_tag="VGB85_31165"
     CDS             complement(1786..>2202)
                     /locus_tag="VGB85_31165"
                     /inference="COORDINATES: protein motif:HMM:NF036850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138600.1"
                     /translation="LLVPGGRVRWVSRDPGALAAVLATRPWLDDIVMPAAYGTGAVRG
                     AQLVVNCTTVGMRDGPSSFPAPLELAALAAGARVIDAVYPRSPGGLLDQVEALGHPTQ
                     DGLPMLLWQGVRALERWLEQTVPDEAVAAMRAALGS"
BASE COUNT          316 a          786 c          735 g          365 t
ORIGIN      
        1 cgcgtagcgg tcctgcgcga ggggctggcg cgccggcaca gccgcggtgc cgagcggcat
       61 gtgtgccgcg gccccgtggc cctgcaccgg catgtgcccg tgcgtgtggc tggcgctggc
      121 gctctggttc ccgccgccga gcagcgggtt gttcccgagg ttgttgccga gcagacccgg
      181 gcccggcatg cccgggacct gcacctgcga ctgccggcgg ccgcgcgaca cggccccggt
      241 cgagtgggcg cggatcacct gctcgagctc gtcctcggcc ggcgcgtggc ggtcgaagcc
      301 ggtggcgatg acggtcagcc gcagcgcgtc gcccatgttg gggtcgatga cggcgccgaa
      361 gatgatgttg cagtcctcgt gggcggcctc ctggatcagc gacgcggcct cctcgacctc
      421 gtgcagcttg aggtccggac cggccgtgat gttgatcagg atgccctgcg cgccgtggat
      481 cgtgacatcc tcgagcagcg gcgagctgat cgcctgctgc gcggcctgca gcgcgcgacc
      541 ctcgtcgtgg ccgatgccca tgcccatcag ggcgcggccc tggccggaca tgatcgtgcg
      601 cacgtccgcg aagtcgacgt tgatgatgcc cggcaccgtg atcaggtccg agacgccctg
      661 cgtcgcgtgc tgtagcacct cgtccgcgag gcggaacgag tcgagcaggc tcgtgttggc
      721 gctcgtcacc tgcaacaggc gatcgttcgg gatcgtgatg agcgtatcga cggccttctc
      781 gagccgctcg atgccctcct ccgcgaagcg caggcgacgc ttgccctcga agcggaacgg
      841 cttggtgacc acgccgacgg tcagcgcgcc catctcacgg gcgatctgcg cgatgatcgg
      901 cgcggcgccg gtgcccgtgc cgccgcccat gcccgcggtg acgaacacca tgtcggcacc
      961 cttgagcgcc tcggcgatct ccgcgatgct ctcgagcgcc gcctcgcggc ccttctccgg
     1021 gttggcgccc gctccgagcc cgcgcgtgat gtcgcggccc agctggatca cggtcggcgc
     1081 gagactcctc tccagcgcct gcacatcggt gttggcgacg tagaattcca cgccctccac
     1141 cttgttctgg atcatggtgt tgacggcgtt acccccggct ccgcccaccc cgatcacctt
     1201 gatcttcgcc tgatcttgca ccgcgtcctc gagttcaaag tgcgccatag ctgtgaccga
     1261 cctgctgtct atgcaggcat tatccgaggc gatccaacat tgcaaaaaat ttgtgatcgc
     1321 cgatcgcgca ggtaatcctg tgaagtttta tgtcccacat gtaggacatt tacggtgcgc
     1381 cttagtgttt catccaaatg gccaccacag gcccgtaaac cccggatctc gcgatctccg
     1441 gcccctcacc gcggctgtcc gtaagatcag gtttgctcgc ccgaccgggc ctgcaccgat
     1501 ctcggtcgcg atcggccaat cgtcgctgcg ggcgtggttc ccgcgggtcg cgatcgccgg
     1561 atattcgtag cagatcgaat tcagggagat cggacactcg ctcgcgatcc acacgcgatc
     1621 caccgcctgt ccgatcggac aggaacagtg atcagatctt caggtgccgc acggacgcga
     1681 aaaactcacc tggcccttgg gtctggatcc aagggacagg tcggtcggga caggcggcga
     1741 tcgcagctag cccgccgcgg agcccgtgtc caatttttgg cgaagtcagc tgccgagcgc
     1801 ggcgcgcatg gcggcgaccg cctcgtcggg cacggtctgc tcgagccagc gctcgagggc
     1861 ccgcaccccc tgccacagca gcatcggcag gccgtcctgc gtgggatgac cgagcgcctc
     1921 gacctggtcg agcaggccgc ccgggctgcg cggatacacc gcgtcgatca cccgcgcccc
     1981 cgcggcgagg gccgccagct cgagcggcgc cgggaagctc gacggcccgt cgcgcatgcc
     2041 gaccgtcgtg cagttgacca ccagctgcgc gccacgcacg gccccggtcc cgtaggcggc
     2101 cggcatcacg atgtcgtcga gccacggccg cgtcgccagc accgccgcga gcgcgcccgg
     2161 gtcgcggctg acccagcgca cgcggccgcc ggggaccagc ag
//
LOCUS       DASWNJ010001870         5487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_527120, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001870 DASWNJ010000000
VERSION     DASWNJ010001870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527120"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VGB85_31170"
     CDS             <1..810
                     /locus_tag="VGB85_31170"
                     /inference="COORDINATES: protein motif:HMM:NF025185.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEY0138601.1"
                     /translation="WRRALGLGLAGLLLVWSLWLPGGPLLRGIVALLAFWGAARVLDL
                     ARDSRPHSARERVAFVFALIDTRTLEWQAPRVDIAAFGKAAAWIPIGLLAVTTAVHLH
                     DPGPVRWGLGALTIYALGEAMEGLLRGLLGLLGATSPPLHRTPIAARSLREFWGERWN
                     LVVNRWLRVHCYLPLARRQRPVLGLAVAFLASAAIHAWFIGVALGPAMAGRMGLYFVI
                     QGLLLVAEQPLGVARWPAPAGRAWMLACTLLPSPLFVEPVLRLFARPLVFV"
     gene            1118..2827
                     /locus_tag="VGB85_31175"
     CDS             1118..2827
                     /locus_tag="VGB85_31175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138602.1"
                     /translation="MNTKYLKPLAVTLGLGLLPACPSSPSSTTEAMDTTQGTTGDATT
                     GDPDVSTTTTGTPTSEPTTTGGTTTGDDSTTGDPTTGTTGEDTTTGPTGTLCERLGGG
                     WGVAELVDSFLLVVAQDEKINGYFLNTDVDAGSLATMVSAQLGELAGCEGVVYGGLGM
                     QEAHAGMGISAQDFVDFVADFQQALTAHAATHPELSEADQAAVLAVLGGFEAEIVEDP
                     ENNLTVYQRVGRKPAIKQMVGGPDDPASFIAILLADDAINTYFVMTDVVRFNTCLTRQ
                     LGDIDGPIQYGAEVDSPGPGIDEGASLVDPCRDMSLVHENLQDAAMTYITVDDFGAMM
                     ADLATAMSLADVSDSDQALVLAAVWPLCDAIVVGSEEKNKCPGNSKQETVELTALAQP
                     LKDNAYDGSLDSMLCTDVVVAQDAAGIELVSAVELTLGLDHPWIGDVNVKIESPTGAI
                     LTVFNRPGNLVMPDDGTGCCNDNSDLSKSYPIVLKNGGMFDAAKMGTTIDAGKVICKD
                     DKQCEFKPNHGSGPGTDFTDFLGETAAGTWKVCIGDSNAGDLGVLDHVGLTFTKVKYD
                     PKL"
     gene            complement(2868..4391)
                     /locus_tag="VGB85_31180"
     CDS             complement(2868..4391)
                     /locus_tag="VGB85_31180"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEY0138603.1"
                     /translation="MSAARIRSLLLLLVVWPLKALWAASVVAVPAIGVWVASSTAAYR
                     NGPVWLACLCGLLLFPLLPLLWEGLGTWRRSRKGKPRPRILTFFDRLILRTLVINLGV
                     LAVLLARDPETTFAALSTRGDWFLDGREGPRVDRARAGLFFAADRLQWLYEAVHDNAY
                     EEVLRDEVPPPPRPEPVPEDSKPGTKVEPVPTPVPTPVPTPTPETPTPTPTPQFAWPM
                     PDRLHPLVVGMPAEAEASIAAVGKYIAEREPDRRMRIKALHDYVADRVVYDGASYMAR
                     GEPDFVFPPQDAETVFRKRLGVCAGYARLLAALGDAAGEEIVVVGGDARTRGSDVTGE
                     SHAWNAARIDDTWHLIDSTWDSGSLDDRGNFKKAYSTAYLFAPPEVFAVSHFPEQENW
                     QLLAKPRSRGEFFRGPMMRADFFAQGLELIRPDRSQVSVDEVFELAVKNPRGLFFMAK
                     FVSPNGTSGDCEVTQGKTAAARCTLPAAGTYQVDLYAAPQRYGSYPNVGAFEVLRRD"
     gene            complement(4476..>5487)
                     /locus_tag="VGB85_31185"
     CDS             complement(4476..>5487)
                     /locus_tag="VGB85_31185"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0138604.1"
                     /translation="FVELLHPQDMEVTIAASQELANGKPAIAFENRYRHKDGSYRHLR
                     WFATPSDANGIQHGVAQDISELRIAEQRARVYEDTILNSSTGMFVCQVEQPGDPTSLR
                     LIMANEASEKFTGVPLRSELGRRFDEIFPQIAQTKLPQAYMEVAMAGGAVDLGEVVYG
                     DDRVEQSHFSVKAYGVTGDRVCVNFENITERKKAEEVRLRSIRQDEIIRAQAAALAEL
                     STPLIPVSDEVVVMPLIGTLDARRAAQVLDTLLSGIASRRATTAILDITGVSGVDTET
                     ANALIRAARAVSLLGARVMLTGIRPEIARMFVELNVDLTTIDTYGSLQTGIAAAFAAR
                     RG"
BASE COUNT          803 a         1983 c         1919 g          782 t
ORIGIN      
        1 tggcgccgcg cgctcgggct cggcctcgcg gggctgctgc tcgtgtggtc gctgtggctg
       61 ccgggcggcc cgctgctccg cggtatcgtc gcgctgctgg cgttttgggg cgcggcgagg
      121 gtgctcgacc tcgccaggga ctcgcgcccc cacagcgcgc gcgagcgcgt cgccttcgtg
      181 tttgcgctca tcgacacgcg cacgctcgag tggcaggcgc cgcgcgtcga catcgccgcc
      241 ttcggcaagg ccgccgcgtg gatcccgatc ggtctgctcg ccgtcaccac cgccgtgcat
      301 ctgcatgacc cggggccggt ccgctggggg ctgggggccc tgacgatcta cgcgctcggt
      361 gaggcgatgg agggtttgct gcgcggcctg ctcggcctgc tcggcgcgac gagcccgccg
      421 ctgcaccgca cgccgatcgc ggcgcgatcg ctgcgcgagt tctggggcga gcgctggaac
      481 ctcgtcgtca accgatggtt gcgcgtgcac tgctatctgc cgctcgcgcg ccggcagcgg
      541 cccgtgctcg ggctcgccgt cgcgttcctc gccagcgctg cgatccacgc gtggttcatc
      601 ggcgtcgcgc tcggcccggc gatggcgggg cgcatgggcc tgtacttcgt gatccagggg
      661 ctgctgctgg tggccgagca gccgctcggc gtggcccgct ggcccgcgcc ggccggccgc
      721 gcgtggatgc tggcgtgcac gctgctgccc tcgccgctgt tcgtcgagcc ggtgctgcgg
      781 ctgttcgcgc ggccgctcgt gttcgtgtag cgatcgacga gcaccgcgtg cgtgcaccgg
      841 tcgttgaaca ccagcggcag gccgcgccgc cgcagctgcg gcaccaggcg gtcggctcgt
      901 gcccctttct gccagtggcg agccgacgtc cccggatggc gacgattcgc cgggaatagc
      961 aggaccggcg ggacccgcat ctgcgggctc gccccctgcc ctgcacaccc tcgcctcgct
     1021 aaaaagcgcg cccggcagtc acaagcgcgc gcgcatctgt atcatgcccg cgcccggccc
     1081 accggccgct cccctttttc gcgcaggacg ctggcaaatg aacacgaagt acctcaagcc
     1141 actcgccgtg acgctcggcc tcggtctgct gccggcctgt ccgtcctccc cgagctcgac
     1201 caccgaggcc atggacacga cgcagggcac gaccggcgac gcgaccacgg gcgacccgga
     1261 cgtctccacg accacgaccg ggacgccgac gagcgagccg acgacgaccg gcggcaccac
     1321 gaccggcgac gacagcacga ccggcgatcc gacgaccggg acgaccggcg aggacacgac
     1381 cacgggcccg accggcaccc tgtgcgagcg gctcggcggc ggctgggggg tcgccgagct
     1441 ggtcgacagc tttttgctcg tcgtcgccca ggacgagaag atcaacggct acttcctcaa
     1501 cacggacgtc gacgccggca gcctggcgac gatggtcagc gcgcagctcg gcgagctcgc
     1561 cggctgcgag ggcgtcgtgt acggcggcct gggcatgcag gaggcgcacg ccggcatggg
     1621 catctcggcg caggactttg tcgacttcgt cgccgacttt caacaggcgc tcacggccca
     1681 cgccgcgacc caccccgagc tgagcgaggc cgaccaggcc gcggtgctcg cggtgctggg
     1741 cggcttcgag gccgagatcg tcgaggaccc cgagaacaac ctcaccgtgt accagcgggt
     1801 cggtcgcaaa ccggcgatca agcagatggt cggcgggccc gacgatccgg cctcgttcat
     1861 cgccatcttg ctcgccgacg acgcgatcaa cacctacttc gtgatgaccg acgtcgtccg
     1921 cttcaacacc tgcctgacgc ggcagctcgg cgacatcgac gggccgatcc agtacggcgc
     1981 cgaggtcgac tcgcccgggc ccggcatcga cgagggcgcc tcgctcgtcg acccgtgccg
     2041 cgacatgagc ctggtacacg agaacctgca ggacgcggcg atgacctaca tcaccgtcga
     2101 cgacttcggc gcgatgatgg ccgacctcgc cacggcgatg agcctcgccg acgtgtccga
     2161 cagcgaccag gcgctcgtgc tcgcggccgt gtggccgctg tgcgacgcga tcgtcgtcgg
     2221 cagcgaggag aagaacaagt gcccgggcaa cagcaagcag gagacggtcg agctgaccgc
     2281 gctcgcccag ccgctcaagg acaacgccta cgacggctcg ctggactcga tgctgtgcac
     2341 cgatgtcgtc gtcgcccagg acgccgccgg catcgagctg gtcagcgccg tcgagctcac
     2401 gctcgggctc gaccacccgt ggatcggtga cgtcaacgtc aagatcgaga gcccgaccgg
     2461 cgcgatcctc acggtgttca accgccccgg taacctggtc atgccggacg acggcaccgg
     2521 gtgctgcaac gacaactccg acctcagcaa gagctacccg atcgtgctca agaacggcgg
     2581 catgttcgac gccgccaaga tgggcaccac gatcgacgcc ggcaaggtca tctgcaagga
     2641 cgacaagcag tgcgagttca agcccaacca cggcagcgga ccgggcaccg acttcaccga
     2701 cttcctcggg gagaccgccg cgggcacctg gaaggtgtgc atcggcgaca gcaacgccgg
     2761 tgacctcggc gtgctcgacc acgtcggcct caccttcacc aaggtgaagt acgacccgaa
     2821 gctatgatga agctatgatg aagctatgat gtcgccggac cggcggctca gtcgcggcgc
     2881 agcacctcga acgcgccgac gttcgggtag ctgccgtagc gctgcggcgc cgcgtagagg
     2941 tcgacctggt aggtgcccgc ggccggcagc gtgcagcggg ccgccgcggt cttgccctgc
     3001 gtgacctcgc agtcgccgct ggtgccgttg ggcgacacga acttcgccat gaagaacagc
     3061 ccgcgcgggt tcttgaccgc cagctcgaac acctcgtcga cgctgacctg cgagcggtcg
     3121 gggcggatca gctcgaggcc ctgggcgaag aagtcggcgc gcatcatcgg cccgcggaag
     3181 aactcgccgc gcgagcgcgg cttggccagc agctgccagt tctcctgctc cgggaagtgc
     3241 gagacggcga acacctccgg cggcgcgaac aggtacgcgg tgctgtacgc ttttttgaaa
     3301 ttgccgcggt cgtcgaggct gccgctgtcc caggtcgagt cgatcaggtg ccaggtgtcg
     3361 tcgatgcgcg cggcgttcca cgcgtggctc tcgccggtga cgtcgctgcc gcgggtgcgc
     3421 gcgtcgccgc cgacgaccac gatctcctcg cccgcggcgt cgccgagcgc ggcgagcagg
     3481 cgcgcgtagc cggcacacac gccgagccgc ttgcgaaaca cggtctcggc gtcctgcggc
     3541 gggaacacga agtcgggctc gccgcgggcc atgtacgagg cgccgtcgta cacgacgcgg
     3601 tcggcgacgt agtcgtgcag cgccttgatc cgcatgcgcc ggtcgggctc gcgctcggcg
     3661 atgtacttgc cgaccgcggc gatgctcgcc tcggcctccg ccggcatgcc caccaccagc
     3721 gggtgcagcc ggtccggcat cggccaggca aactgcggcg tcggcgtcgg cgtcggcgtc
     3781 tcgggcgtgg gggtcggcac cggcgtcggc accggcgtcg gcaccggctc gaccttcgtg
     3841 cccggcttgc tgtcctccgg gacaggctcg gggcgcggcg gcggcgggac ctcgtcgcgc
     3901 aagacctcct cgtaggcgtt gtcgtgcacg gcctcgtaca gccactgcag gcggtccgcg
     3961 gcgaagaaca ggccggcgcg cgcccggtcg acgcgcggcc cctcgcggcc gtcgaggaac
     4021 cagtcgccgc gggtcgacag cgccgcgaag gtggtctccg ggtcgcgcgc cagcagcacc
     4081 gcgagcaccc cgaggttgat caccagcgtg cgcaggatca ggcgatcgaa gaaggtcagg
     4141 atccgcggcc gcggcttgcc cttgcggcta cgccgccagg tgccgagccc ctcccacagc
     4201 agcggcagca gcgggaacag cagcagcccg cacaggcacg ccagccacac cggcccgttg
     4261 cgataggcgg cggtcgagga cgcgacccac acgccgatcg caggcaccgc caccaccgac
     4321 gccgcccaca gcgccttgag cggccagacg acgagcagga gcagcagcga ccggatccgg
     4381 gcagcggaca tgaacggcca gcatagcagc cggcgatccc gcgtcgaccc gcccgccgtc
     4441 ggccccgcgc gcagtgccgg cgcgccgggc tcgactcagc cgcgccgcgc cgcgaaggcc
     4501 gcggcgatgc ccgtctgcag cgagccgtac gtatcgatcg tggtgaggtc gacgttgagc
     4561 tcgacgaaca tgcgcgcgat ctcgggccgg atgccggtca gcatcacgcg cgcgccgagc
     4621 agactcaccg cgcgggccgc ccggatcagc gcgttggcgg tctcggtgtc gacgcccgac
     4681 acaccggtga tgtcgaggat cgccgtcgtc gcgcggcggc tggcgatgcc ggagagcagc
     4741 gtgtcgagca cctgcgcggc ccgccgggcg tcgagggtgc cgatcaaagg catcacgacg
     4801 acctcgtcgc tgaccgggat cagcggggtc gacagctccg cgagcgcggc ggcctgggcc
     4861 cggatgatct cgtcctgacg gatgctgcgc agccgcacct cctcggcctt cttgcgctcg
     4921 gtgatgttct cgaagttgac gcacacgcgg tcgcccgtga cgccgtacgc cttgaccgag
     4981 aagtggctct gctcgacgcg gtcgtcgccg tacaccacct ccccgaggtc gaccgcaccg
     5041 cctgccatgg cgacctccat gtacgcctgc ggcagcttcg tctgtgcgat ctgcgggaag
     5101 atctcgtcga agcgccggcc gagctcggag cgcaggggca cgcccgtgaa cttctcgctc
     5161 gcctcgttcg ccatgatcag ccgcagcgag gtcgggtcgc cgggctgctc gacctggcag
     5221 acgaacatcc ccgtggacga gttgaggatc gtgtcctcgt agacgcgcgc gcgctgctcg
     5281 gcgatccgca gctcgctgat gtcctgggcg accccgtgct ggatcccgtt cgcgtcgctc
     5341 ggcgtcgcga accagcggag gtggcggtac gacccgtcct tgtggcggta gcggttctcg
     5401 aacgcgatcg cgggcttgcc gttcgcgagc tcctggctcg ccgcgatcgt cacctccatg
     5461 tcctgcggat ggagcagctc gacgaag
//
LOCUS       DASWNJ010001871         5918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_598222, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001871 DASWNJ010000000
VERSION     DASWNJ010001871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598222"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1507)
                     /locus_tag="VGB85_31190"
     CDS             complement(<1..1507)
                     /locus_tag="VGB85_31190"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0138605.1"
                     /translation="MTSPETTPAALLVTPPAGGFDRAGVRDLLKESGLATALHRLVRA
                     PFGHTVLTLRALDAVIAAGDLALQAGESLHAALLEDIARAGSLAIPEPTADQRLFIGA
                     FTVTVLADSLAPALAELAPSPELHTDLEADGLEDLLRKTPRATVQTLLGMAGKYLEVQ
                     AQRQPGAAAPKLDERELWVVTSLHAFVLQLQGAVRRLTHLGRLRPFGVALARRKVVVG
                     ELRYEGFAARNLAEPVSDLKPIKIEEVVGNQEYLQAGLKLARDVAAFDLAARRGPKQL
                     NPVLFGLGRPGCGKTITAHAIGNYFLEYCRQRDIPARFKVIRRTDWASSYQNASAATL
                     VKIFKEEVYGFEGVCGVYWPDIDTAFASRSSGDLRSEEKNNLGAVFGIFDGTLIPKDG
                     KWFMICDANFMQMDEATRSRIAQNPFTVKGPTTSGDYVHLLRDVLLGELRAFVAVGEE
                     ARWPEVGAALVAADLSGRSVESIAGNIRAAIQDFEYPDEYFKASFDERRRII"
     gene            complement(1559..2035)
                     /locus_tag="VGB85_31195"
     CDS             complement(1559..2035)
                     /locus_tag="VGB85_31195"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HEY0138606.1"
                     /translation="MNLRLDPTSILNTWKTFADKPFGKQLVSRAVGLMAPYTGSITPQ
                     ILDIEPGHARVQMRDRWGVRNHLRSLHAVALMNLGEACTGLAVMSVVPAGGRGIVLEL
                     GMTYVKKARGTITAESRVTIPETPGSHDLWVEGELFNATGELVARARARWRIEIPA"
     gene            2186..3007
                     /locus_tag="VGB85_31200"
     CDS             2186..3007
                     /locus_tag="VGB85_31200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138607.1"
                     /translation="MVYRDLVSHSLCAAAIGLTLACNPDKGDDTSASDSAVTTGNEST
                     GATTGATTGATTGPTTGPTTDVGTTEVVTTGGGETVTTGATTTTGGTTTTEETTGGET
                     TGGAIGCGEDAEFTASWNAWQTAVQANGATYYYAVIRGVSGFGPPDYCLYRTTVVVTD
                     GVPVERRFEISAMVGNPVDCEEPFTEMGEDLGTHDSGFAAVPATVDTLYGACCDQVIH
                     VEPAEEYMVTFATDDQGLMKQCYYVANGCADGCSGGPFGPSLDFEVLAFGAPPAP"
     gene            complement(3018..3800)
                     /locus_tag="VGB85_31205"
     CDS             complement(3018..3800)
                     /locus_tag="VGB85_31205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138608.1"
                     /translation="MFNGEISPSGPGAVGFDVLGLDTDWGGLAARLRATRAGIGRASP
                     ALAQIDGLIEICATIAACREARVVDALVRPGPDGTYDPGRVRAGLFLLSRYFTVADHG
                     RGDAEAFTLILPLPEQIFRCWALWRLGLRGDRVPPGHDDVWRLVAGEWSGGSFRRPAA
                     GGGFPSLACFAMFAMCKLWIAGVATPDAPESNMRELKRALTLLRRRNDAAHGLMRPEP
                     GLRRDFFALARRWLDRLYLACPLGGGREVQSELAGLTRRLSG"
     gene            3992..>5918
                     /locus_tag="VGB85_31210"
     CDS             3992..>5918
                     /locus_tag="VGB85_31210"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0138609.1"
                     /translation="MHSTAAIEHGHELQALESALLAEHTLVRVGFRAPVFDRRYTLVR
                     PLGRGARGLVWCAHDGKMHREIALKLYPYHPGDRLAEEVTREARALAQLRHPAVVQVF
                     DYGHGSFDSNGRECLYVTMECLDGRPLRVWLGEPRTAAEILAVFLRAGEGLVAAHQRG
                     LVHRDFKPDNVVLERDGSPRIVDFGLASAEIVAARADGPEVSLPGERRPVLGTPAYLA
                     PEAIAGQADARSDQFSFAASLWQALFGSFPYDEHAFDSLSRATLRDPPAREDLNPQIL
                     VALRRALAPSPAARFADMAALLAALREAPQVQVDHEPTAIARPPAPTSRSGPYLLAGF
                     ALIASGAAYGLWPTLEPAVDEPAQRPVPAPPGPTCELDELLAGDWQYHTSVEWSEFID
                     LIGVSGQYRVGFTTQDNCRYDTLVTKLGDSGNAYPNARRDRVSAQASKLPGFDAHLAA
                     ELWLAQRSDAELAQPTAPAVRRRGAHYLVEYTVDGARLVGHWRYFAEKDGHVLMTGAL
                     RGGRTAPTSVPAGIADLPCRSQCALLCAGGQAEARCVQDRCRDPRAAVQDCGAPDPDF
                     AASPQTEPGSPLHYYNPDTDRKQPDRCLQIAERLAGAWTFHLRAATGAHAGEATEHQV
                     TLRVAGCTLRGTARDRGV"
BASE COUNT          866 a         2292 c         1934 g          826 t
ORIGIN      
        1 cgatgatgcg ccgccgttca tcaaagctcg ccttgaagta ttcgtccggg tattcgaagt
       61 cctggatcgc cgcgcggatg ttgccggcga tgctctccac ggagcggccg ctgaggtccg
      121 ccgcgacgag cgccgcgccg acctcgggcc agcgcgcctc ctcgccgacc gccacgaacg
      181 cgcgcagctc accgagcagc acgtcgcgca gcaggtgcac ataatctccg gaggtcgtcg
      241 gccccttcac ggtaaacggg ttctgcgcga tccgtgagcg cgtcgcctcg tccatctgca
      301 tgaagttcgc gtcgcagatc atgaaccact tgccgtcctt ggggatcagc gtgccgtcga
      361 agatgccgaa caccgcgccg aggttgttct tctcctcgga gcgcaggtcg ccgctcgagc
      421 gcgacgcgaa cgccgtgtcg atgtccggcc agtagacccc gcacaccccc tcgaagccgt
      481 acacctcctc cttgaaaatt ttgaccagcg tcgccgcgct cgcgttctga tacgagctgg
      541 cccagtcggt gcgccggatg accttgaagc gggccgggat gtcgcgttgc cggcaatact
      601 ccaaaaaata gttgccgatc gcgtgggccg tgatcgtctt gccgcagccc gggcggccga
      661 ggccgaacag caccgggttg agctgcttgg gcccgcggcg ggccgcgagg tcgaaggcgg
      721 cgacgtcacg cgcgagcttg agccccgcct gcaggtactc ctgattgccg acgacctcct
      781 cgatcttgat cggcttgagg tccgacaccg gctccgcgag gttgcgcgcc gcgaagccct
      841 cgtagcgcag ctccccgacc accaccttgc gccgcgccag cgccaccccg aacggccgca
      901 gccggcccag gtgcgtcagg cggcgcaccg ccccttgcag ctgcaacacg aatgcatgca
      961 ggctcgtcac cacccacagc tcgcgctcgt cgagcttcgg cgccgccgcg cccggctggc
     1021 gctgcgcctg cacctcgagg tacttgcccg ccatcccgag cagcgtctgc accgtcgcgc
     1081 gcggcgtctt gcgcagcagg tcctcgagcc cgtcggcctc gaggtcggtg tgcagctcgg
     1141 gcgagggggc cagctccgcg agcgcgggcg cgagcgaatc cgcgaggacc gtcaccgtga
     1201 acgcgccgat gaacagccgc tggtccgccg tcggctcggg gatcgccagc gagcccgcgc
     1261 gcgcgatgtc ctcgagcagc gcggcgtgca gcgactcgcc ggcctgcagc gccaggtcac
     1321 cggccgcgat cacggcatcg agcgcgcgca gcgtcagcac cgtgtgcccg aagggcgcgc
     1381 gcaccaggcg atgcagcgcc gtcgccaggc ccgactcctt gagcaggtcc cgcacgcccg
     1441 cgcggtcgaa gcctcccgcg ggcggcgtga cgaggagcgc ggcaggagtg gtctcgggcg
     1501 atgtcatgcg cgcccagcct aacgcagcgg ggccgcggcg cggaagcccg aacccgacct
     1561 acgccgggat ctcgatgcgc cagcgggcgc gggcgcgcgc gaccagctcg ccggtggcgt
     1621 tgaacagctc gccctcgacc cacaggtcgt gcgagcccgg cgtctccggg atcgtgaccc
     1681 gcgactcggc cgtgatcgtg ccgcgggcct tcttcacgta ggtcatgccg agctcgagca
     1741 cgatgccgcg accgccggcc ggcacgacgc tcatcacggc gagcccggtg cacgcctcgc
     1801 cgaggttcat cagcgcgacc gcgtgcaggc tccgcaggtg gttgcgcacg ccccagcggt
     1861 cgcgcatctg cacgcgcgcg tgtccgggct cgatgtcgag gatctgcggc gtgatcgacc
     1921 cggtgtaggg cgccatcagg ccgaccgcgc gcgacaccag ctgcttgccg aagggcttgt
     1981 cggcgaaggt cttccaggtg ttgaggatcg aagtcgggtc gaggcgaagg ttcatggcgg
     2041 gatctcgggc tcggggcgct ggatagtgcc ccggtcggcc gaccggcaca agacccgcac
     2101 acgctcgccg gcctgatcgc agcgccctgc catgacggat ctgtcagtct ggacaggatc
     2161 gcggcggctg ctacgttgcg cccccatggt ttaccgcgac ctcgtctccc attccctgtg
     2221 cgccgctgcg atcggcctca ccctcgcctg caaccccgac aagggcgacg acacctccgc
     2281 cagcgactcc gccgtcacca ccggcaacga gtcgaccggc gccaccaccg gcgccaccac
     2341 cggcgccacc accggtccga ccaccggccc gaccaccgat gtcggcacca ccgaggtcgt
     2401 caccaccggc ggcggcgaga ccgtgacgac cggggcgacc acgaccactg gcggcaccac
     2461 cacgaccgag gagaccaccg gcggcgagac caccggcggc gcgatcggct gcggcgagga
     2521 tgccgaattc accgccagct ggaacgcctg gcagacggcc gtgcaggcca acggtgccac
     2581 ttactactat gccgtgatcc gcggcgtcag cggcttcggc ccgcccgact attgtctgta
     2641 tcgcaccacg gtcgtcgtca ccgacggtgt tcccgtcgag cgtcgcttcg agatctccgc
     2701 gatggtcggc aacccggtcg actgcgagga gcccttcacc gagatgggcg aggacctcgg
     2761 cacccacgac tccggcttcg ccgccgtgcc cgccaccgtc gacaccctct acggcgcctg
     2821 ctgcgaccag gtcatccacg tcgagcccgc cgaggagtac atggtcacct tcgcgaccga
     2881 cgaccagggc ctgatgaagc agtgctacta cgtcgccaac ggctgcgccg acggctgctc
     2941 cggcggcccc ttcggcccga gcctcgactt cgaggtcctc gccttcggcg ccccgccggc
     3001 cccgtgaggc ggcgctgtca acccgacagc cgccgcgtca gacctgcgag ctcgctctgg
     3061 acctcgcgac cgccgcccag cggacacgcg aggtacagcc ggtcgagcca gcgccgcgcc
     3121 agcgcgaaga agtcccggcg cagcccgggc tccggtcgca tcaatccatg cgccgcgtcg
     3181 ttacggcggc gcagcagcgt gagcgctcgc ttgagctcgc gcatgttgct ctcgggcgcg
     3241 tctggcgtcg cgaccccggc gatccacagc ttgcacatcg cgaacattgc gaagcaggcc
     3301 agcgacggga acccgccccc ggccgccggc cggcgaaacg aaccgccgct ccactcgccg
     3361 gcgacgagcc gccagacatc gtcgtgcccg ggcggcacac gatcgccgcg caggccgagc
     3421 cgccacaacg cccagcagcg aaagatctgc tccggtaacg gcaggatcag cgtgaacgcc
     3481 tcggcgtccc cgcgcccgtg atccgcgacg gtaaaatatc gcgataataa aaacagcccg
     3541 gcgcgcacgc gccccgggtc atatgtccca tcgggcccgg gtcggaccag cgcgtcgacc
     3601 actcgcgcct cgcggcacgc cgcgatcgtc gcgcagatct cgatcagccc gtcgatctgc
     3661 gcgagtgccg gcgaagcgcg cccgatcccc gcgcgggtcg cccgcaacct cgccgcgagc
     3721 ccgccccagt cggtgtcgag cccgagcacg tcgaagccga ccgcccccgg ccctgacggc
     3781 gaaatctcgc cattgaacat cgttatatag ccagtcctgc cgatatcatc catcgtcgaa
     3841 gctccccgcc acggcccgcg cgatggcggg tccatgtctc ctcccgcggc gccgtcggtt
     3901 cggctcaacg gggaattcgc ctgccgcgac ggcgccgcaa ttttcgcgcc ggcctcccgc
     3961 cgcgtgcgcg ctggcataca ctcgccgttc gatgcactcc acggccgcca tcgagcacgg
     4021 gcacgagctg caggccctcg agagcgcgct gctggccgag cacacgctgg tacgtgtcgg
     4081 ctttcgtgcg ccggtgttcg accgccgtta cacgctggtg cgcccgctcg gccgcggcgc
     4141 ccgcggcctc gtttggtgcg cccacgacgg caagatgcac cgcgagatcg cgctcaagct
     4201 gtatccctat catccgggcg accgcctcgc cgaggaggtc acgcgcgagg cccgggcgct
     4261 cgcgcagctg cgccatcccg cggtcgtgca ggtcttcgat tacggtcacg gcagcttcga
     4321 cagcaacggc cgcgaatgcc tctacgtgac gatggagtgc ctcgacggcc ggccgctgcg
     4381 cgtctggctc ggcgagccac gcaccgccgc ggagatcctc gcggtgttcc tacgcgcggg
     4441 cgagggtctc gtcgccgctc accagcgcgg cctcgtccac cgcgacttca agccggacaa
     4501 cgtcgtgctc gagcgcgacg gcagcccgcg gatcgtcgac ttcgggctcg ccagcgccga
     4561 gatcgtcgcc gcccgggccg acggacccga ggtctccttg cccggcgagc gtcggcccgt
     4621 gctcggcacg cccgcgtacc tcgcccccga ggcgatcgcc ggtcaggccg acgcgcgcag
     4681 cgatcaattt tcgttcgccg cctcgctgtg gcaggcgctg ttcggcagct ttccatacga
     4741 cgagcacgcc ttcgacagcc tcagccgcgc caccttgcgc gacccacccg cgcgcgagga
     4801 tctcaatccg cagatcctcg tcgccttgcg ccgcgccctc gcgccctcgc cggctgctcg
     4861 cttcgccgac atggccgcgc tgctcgccgc gctgcgcgaa gcgccgcagg tccaggtcga
     4921 tcacgaaccg acggcgatcg cccgcccgcc cgcgcccacc tcgcgcagcg gaccgtattt
     4981 actcgccggc ttcgccctga tcgccagcgg cgcggcctac ggcttgtggc cgacgctcga
     5041 gcccgcggtc gacgagcccg cgcagcgccc ggtgcccgcg ccgccgggcc cgacctgcga
     5101 gctcgacgag ctgctcgccg gagactggca gtatcacacc tccgtcgaat ggtcggagtt
     5161 catcgacctg atcggcgtct cggggcaata ccgggtcggc ttcacgaccc aggacaattg
     5221 ccgctacgac accctcgtca ccaagctcgg tgacagcggc aatgcctacc ccaacgcgcg
     5281 ccgcgaccgc gtctcggcgc aggcgagcaa gctgccgggc ttcgacgccc acctcgcggc
     5341 cgagctgtgg ctcgcccagc gtagcgacgc cgagctcgcg cagccgaccg cacccgccgt
     5401 gcgtcgacgc ggcgcgcatt acctcgtcga atacacggtc gacggcgcgc gcctcgtcgg
     5461 tcactggcgc tacttcgcgg agaaggacgg ccacgtattg atgaccggcg cactgcgtgg
     5521 cggtcgcacg gcgccgacca gcgtccccgc gggtatcgcc gacctgccgt gccgctccca
     5581 gtgcgccctg ctgtgcgcgg gcggccaggc cgaggctcgc tgcgtgcagg atcgatgtcg
     5641 ggacccgcgc gccgccgtgc aagattgcgg cgccccggac cccgacttcg ccgcctcgcc
     5701 tcagaccgag cccggcagcc ccctgcatta ttacaacccc gacaccgatc gtaagcagcc
     5761 ggaccgctgt ctgcagatcg ccgagcgcct cgccggcgcg tggaccttcc acctgcgcgc
     5821 ggccaccggt gcgcacgcag gcgaggcgac cgagcatcag gtcacgctgc gcgtcgccgg
     5881 ctgcaccctg cgcggcaccg cccgcgaccg cggtgtcg
//
LOCUS       DASWNJ010001872         4638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_492557, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001872 DASWNJ010000000
VERSION     DASWNJ010001872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492557"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VGB85_31215"
     CDS             <1..637
                     /locus_tag="VGB85_31215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138610.1"
                     /translation="KLEDEILTVDDKLKTFDVLPDVHYGLVVFVDDAQIIDAGAPYLT
                     VEDLKADFNYYWMFTQSNSQVSGDGFNGDFPENTLDALFIAAGGFQWRPVEDTLRLVI
                     HTTDDTFGEQGAVQSGVTIQHTYDETVLALQDKQIRVFAFADNDMTGGPADNEDVSMG
                     FFTPYKGKTPIPEATDGGAFNINLVNKNELSLSAAINESVEQSLCELYIPM"
     gene            681..1256
                     /locus_tag="VGB85_31220"
     CDS             681..1256
                     /locus_tag="VGB85_31220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138611.1"
                     /translation="MGITAHFFAFDPRVCTTPPTIDQLVKSGAVDHELQVLGEAARWL
                     KEIASPLGDNKRWYDNLAGDFAWSCAREHVAPEDRSPLDHWFSHLFWHGAADGRCPCG
                     REPAVVAENEEVYDSALLEHILSLERPLWVVEPALAIEFNGDPPKTERLDRPWIYDFD
                     NFCDLALMWQHLIRRATKAGPGWSLLQWLSP"
     gene            complement(1438..2679)
                     /locus_tag="VGB85_31225"
     CDS             complement(1438..2679)
                     /locus_tag="VGB85_31225"
                     /inference="COORDINATES: protein motif:HMM:NF022019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEY0138612.1"
                     /translation="MNIAPGWYTDPQSVTHLRWWDGTAWTAHVTPINAPPTAPVAPAP
                     QRPAEVSGVLERHIAELRAEYERLEAQVVETRELVLLQEVGIYEYAHPLDTSAQYKEA
                     LEALEREIQQCIKSGGAVTGTKKWAINGSEKEGAKMVADFCKLMLRAYNSEADNTVRT
                     LKHYTLKPAIERLQKMRSSISKLGASMKIEITDRYHGLRVSELELTTDYLVKVNEEKE
                     QQRDERARLKEAEAALRDFEREQARLEKERSHYEAALRALKAKGDTAAVETAELKLQE
                     IQSAIDGVIARAANTRAGYVYVISNIGSFGEEVVKIGMTRRLEPMDRVRELGDASVPF
                     RFDVHALIFSDDAVGLETALHQHFAARRVNLVNAHREFFYATPHEVKDALLALRGSLL
                     SFTETPEALEWHQSATIRSTL"
     gene            complement(2681..3688)
                     /locus_tag="VGB85_31230"
     CDS             complement(2681..3688)
                     /locus_tag="VGB85_31230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138613.1"
                     /translation="MRSIEVDARRRQRQSASEEREAMRRQRVLAKREDELAKYAENVR
                     AAHEAEQFANYLELLVSVHKDCGPTWDWPAIAREQPPAHPIRMPHSENAARAALAAYK
                     PGFFERFFGGDKPRITALKQAIVEAHRADDAAYMVAVERHQQDYAACTARTAQAQHIL
                     ARNTRAYPTVLQLSGALDELTAFKVEVSLTATEQDAIAFTCSIMDDQMVPREEVKLTA
                     KGKLTTKEIAAGRYWALYQDHVCSAAIRVAREVFAVLPVSRVVVNIGPMRLNTSTGHL
                     ESVTFLAVHIARGELYRINLASIDPSDSMKNFHHRMKFKKTTGFEPVEPITLDEQWIT
                     T"
     gene            complement(3855..4106)
                     /locus_tag="VGB85_31235"
     CDS             complement(3855..4106)
                     /locus_tag="VGB85_31235"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0138614.1"
                     /translation="MPADAYLTTAQVAEAVGVSKATILRWSEQGVLPRPVIVHGGRRG
                     RLARWPLTTPEQGRWVQAQLDAHMTWEEIRDALASRSAG"
     gene            4294..4458
                     /locus_tag="VGB85_31240"
     CDS             4294..4458
                     /locus_tag="VGB85_31240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138615.1"
                     /translation="MALFLLADGATLEVHIPDVADAELHASTARTPGFILRRGRRIVN
                     VGALTALFHE"
BASE COUNT          762 a         1624 c         1413 g          839 t
ORIGIN      
        1 aaagctcgag gacgagatcc tcaccgtcga cgacaagctc aagacctttg acgtgctccc
       61 ggacgtccat tatggcctcg tcgtcttcgt cgacgacgcc cagatcatcg acgccggcgc
      121 cccctacctg accgtcgagg acctcaaggc cgacttcaat tattattgga tgttcaccca
      181 gtcgaactcg caggtcagcg gcgacggctt caatggcgac ttccccgaga acaccctcga
      241 cgccctcttc atcgccgccg gcggcttcca gtggcgcccg gtcgaggaca ccctgcgcct
      301 cgtcatccac accaccgacg acaccttcgg cgagcagggc gccgtccaga gcggcgtcac
      361 gatccagcac acctacgacg agaccgtcct ggccctccag gacaagcaga tccgcgtgtt
      421 cgcgttcgcc gacaacgaca tgaccggcgg ccccgccgac aacgaagatg tcagcatggg
      481 cttcttcacc ccctacaagg gcaagacccc gatccccgag gccaccgacg gcggcgcctt
      541 caacatcaac ctcgtcaaca agaacgagct cagcctcagc gccgcgatca acgagtccgt
      601 cgagcagagc ctgtgcgagc tatatatccc gatgtgacgg cggggctgcg tgggcggcct
      661 gcaagagata cgctctacgc atgggtatca ccgcacactt cttcgccttc gacccgcgcg
      721 tgtgcaccac gccgccgacg atcgatcagc tcgtgaagtc cggcgcggtc gaccacgagt
      781 tgcaagtcct cggcgaggcc gcgcgctggc tcaaagagat cgccagtccg ctcggtgaca
      841 acaaacgctg gtatgacaac ctcgccggcg acttcgcgtg gagctgcgcc cgcgagcacg
      901 tcgcccccga ggaccgcagt ccgctcgacc actggttctc gcacctgttc tggcacggcg
      961 ccgccgacgg ccgctgcccg tgcggccgcg agcccgccgt cgtggccgag aacgaggagg
     1021 tctacgactc cgcgctgctc gagcacatcc tgtcgctcga gcgcccgctg tgggtcgtcg
     1081 agcccgcact ggcgatcgag ttcaacggag atccgccgaa gaccgaacgc ctggaccgcc
     1141 catggatcta cgacttcgac aacttctgcg acctcgccct catgtggcag catttaataa
     1201 ggcgcgcgac gaaggccggc cccggctgga gcctcctgca gtggctctcc ccctgatccc
     1261 gcactaccag gtccgcctgg agaaccggca tgtcggtcca cgactgtcac gccgctggca
     1321 ctcgcactcc agcggaggac cgcctcccga tagctctaga tttcacaacc ttggagaatc
     1381 gacaccggaa tgcaacatcc cgatgtgaga tcgggagcag acacgacgcg ctggcgctca
     1441 gagggtgctc cgaatcgtcg cgctctgatg ccactcgagc gcctccgggg tctcggtgaa
     1501 gctcaggagg ctgcctcgta gcgccagcag agcgtccttc acctcgtgtg gcgtcgcgta
     1561 gaagaactcg cggtgggcat tgacgaggtt gaccctgcga gctgcgaagt gctggtgaag
     1621 ggccgtctcc aggccgacgg cgtcgtcgct gaagatgagc gcgtggacgt cgaagcgaaa
     1681 cggcacggac gcgtccccga gctccctgac ccggtccatc ggctccagcc gtcgagtcat
     1741 cccgatcttg acgacctcct cgccgaacga gccgatattc gagatgacgt agacatagcc
     1801 ggcccgcgta ttggccgctc gcgcgatcac cccgtcgatc gcgctctgga tctcctgcaa
     1861 cttgagctcg gcggtctcga ccgccgccgt gtcccccttc gccttcaggg cgcgaagcgc
     1921 agcctcatag tgcgaccgct ccttctcgag ccgggcctgt tcccgctcga agtcccgcag
     1981 cgcggcctcc gcctccttga ggcgcgcacg ctcgtcgcgc tgctgctcct tctcctcgtt
     2041 cacctttacg aggtagtccg tcgtcagctc gagctcggag acccggaggc catggtatct
     2101 gtccgtgatc tcgatcttca tgctcgcgcc gagcttggag atcgatgagc gcatcttctg
     2161 taggcgctcg atcgccggct tgagcgtgta gtgcttgagg gtgcggaccg tattgtcggc
     2221 ctcgctgttg taggcgcgca gcatcagctt gcagaagtcg gcgaccatct tggcgccctc
     2281 cttctcggag ccgttgatcg cccacttctt cgtccccgtg acagctcccc ctgacttgat
     2341 gcactgctgg atctcgcgct ccagcgcctc gagggcctcc ttgtattgtg cagacgtgtc
     2401 gagcgggtgg gcgtattcgt agatccccac ctcctggagc aggaccaact cgcgggtctc
     2461 gacgacctgg gcttcgagtc gctcgtactc cgcgcgcagc tcggcgatat gccgctcgag
     2521 gacaccggag acctccgccg ggcgttgggg cgccggggcc acgggggccg tcgggggcgc
     2581 gttgatcggg gtcacatggg cggtccacgc cgtcccgtcc caccatcgaa ggtgggtgac
     2641 gctctgcggg tcggtatacc agccgggtgc gatgttcatt tcaggtggtg atccattgct
     2701 cgtcgagggt gatcggctcg accggctcga aacccgtcgt cttcttgaac ttcatccggt
     2761 gatgaaaatt cttcatcgaa tcggacgggt cgatgctcgc caagttgatg cgatacagct
     2821 ctccacgggc gatatggacc gcgaggaagg tgaccgactc gaggtgcccg gtgctcgtgt
     2881 tcaatcgcat cggcccgatg ttgacgacca cccgtgatac cgggaggacg gcaaacacct
     2941 ctctggcgac ccgaatcgcc gcggagcaga cgtggtcctg gtagagcgcc cagtagcgcc
     3001 cggccgcgat ctccttggtc gtcagcttgc ccttggccgt cagcttgacc tcctcccgcg
     3061 gcaccatctg gtcatccatg atgctgcagg tgaacgcgat cgcgtcctgc tcggtcgccg
     3121 tcaagctcac ctcgaccttg aaagccgtca gctcgtcgag ggcgcccgac agttgtagga
     3181 cggtgggata cgctcgcgtg ttgcgcgcga ggatgtgctg ggcctgtgcg gtgcgtgccg
     3241 tgcaggccgc atagtcctgc tggtggcgct ccacggctac catgtatgcc gcgtcgtccg
     3301 cccgatgcgc ctcgacgatg gcttgcttga gcgccgtaat ccgtggtttg tcgccaccga
     3361 aaaaacgctc gaaaaagccg ggcttgtaag cggccagggc cgcccgcgcg gcgttttcac
     3421 tgtgtggcat ccggatcgga tgtgccggcg gctgttcccg ggcgatcgca ggccagtccc
     3481 atgtcggccc gcagtccttg tgtacggata ccagcaactc gagatagttg gcgaactgtt
     3541 cggcttcgtg ggccgctcgc acattctcgg cgtatttggc gagttcgtcc tcgcgcttgg
     3601 cgagtacgcg ctgccgacgc atcgcctccc gctcttcact cgcgctttgc cgctgccgtc
     3661 ggcgagcgtc cacctcgatc gacctcatga tttgacgcca actcatagga ttatcctcgc
     3721 ggtagaaccg ccgctgcgaa tatacgggcg agacgaagcg gccgtggccg cccgcgaaac
     3781 gcgtccatct tgcgaccccg cctgcgcgag cgtcaaccgc aatctacgcg ccactcgatg
     3841 tgtcgcgggc cctgtcaacc cgcggaacgt gacgccagcg cgtcacgaat ctcttcccag
     3901 gtcatatgtg cgtcgagctg ggcttgaacc caccggcctt gctccggcgt ggtcagcggc
     3961 cagcgcgcca gacgcccgcg gcgtccgccg tgcacgatca ccggccgcgg caacacgccc
     4021 tgctccgacc agcgcaggat cgtcgccttg ctgaccccca cggcctcggc cacctgggcg
     4081 gtcgtcagat atgcgtctgc cggcacgagg ccggagtgta cccggacccg tgtttcgcga
     4141 cgggcttcca attaacggtt tgacttgata gaagataagc tgtcaacgag ccgtatacaa
     4201 gcctccaacg agacgacaag caccatggcc agacaacatc gttcccccgg caacgccgca
     4261 cccgcgcctg cctgcccgcc cccctctgct cgcatggcgc tgttcttgct cgccgacggg
     4321 gccacgctcg aggttcacat ccccgacgtc gccgacgccg agctgcatgc ctcgaccgcc
     4381 aggacgccgg gtttcatcct tcgacgcggc aggaggatcg tcaacgtcgg cgcgctgacg
     4441 gctctgtttc acgagtagac gaccggcgct gctcgcagat ctctacctgc gccgcccctg
     4501 cccggtctca cgaggggcgt tgggcccgag caacgcgaca tccgccccgg cttgcagtga
     4561 gagccgcggt cgacggcgac cgcgggcgcc tcaccccccg ccgcgcccgc tcagccgggc
     4621 gggcgcagaa gggtcatg
//
LOCUS       DASWNJ010001873         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_104467, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001873 DASWNJ010000000
VERSION     DASWNJ010001873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104467"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..1215
                     /locus_tag="VGB85_31245"
     CDS             286..1215
                     /locus_tag="VGB85_31245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138616.1"
                     /translation="MRRSLDTAVLSAGLMFGLIGGCYLRPAPAPGFRYSCQETADCQA
                     LDCSGSTISLEKAATLIEGCDSLEVKADPTLGVRARQTCLSGLCEYPCELFTFRLDCP
                     SSEGFQFCLNGACSSLCGTDDYKKYDLDSNDDFCAEGQTCIPFDAAGLDPLVLETFGV
                     GSNSQGGFNIAMLPIGAGMCGARCDAEGARPCPAGQYCTGALCMPGCDQETATPCDAG
                     TKCIALAGFSSCLVACDPNVTGSCGEGEICVPGLDVCQPTCLGPDKVECPDSFECDAT
                     LAICIPIGGDESTGGDTTDGATTGGATTDGATT"
     gene            1232..>1520
                     /locus_tag="VGB85_31250"
     CDS             1232..>1520
                     /locus_tag="VGB85_31250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138617.1"
                     /translation="MTSLRTSFLVAALSLSTACAGLEPQPVRLDARLASLNIGNMGVR
                     NSYELRNASPIAGRATETVPVDDSRPRERRPITPVLFWLGIGLTAVGGVSTI"
BASE COUNT          220 a          571 c          518 g          211 t
ORIGIN      
        1 gagaccctcc agggacatgt ggattgaaac tgtgggcggg ccgtgtagca cgcccgaagg
       61 tgcgcgggca aggcggcagc ggcaggtccg ctgcggtcgg tcgtggcgcc ggggcggcgg
      121 ggttccgccg gatctcggcc acgtgtgagc ctcctcgctg gggcgaaccc ggcgggcccg
      181 cggcggggtc gaagcctgcg aagcccgtgc gcatcctcgc acatgggcgc caggccaggg
      241 gcgtcgcccg cggacgcagg ccactgctat aagccgggag tcctcatgcg ccgcagcctc
      301 gataccgccg tcctgagcgc cggcttgatg tttggattga tcggcgggtg ttacctgcgg
      361 cccgcgccgg cgcccggctt tcgctactcg tgccaggaga ccgcggactg ccaggcgctc
      421 gactgcagcg gctcgacgat ctcgctcgag aaggccgcga ccctgatcga aggctgcgac
      481 tcgctcgagg tcaaggccga cccgaccctc ggcgtccgcg cccgccagac ctgcctgtcc
      541 gggctgtgcg agtacccctg cgagctgttc accttccgcc tcgattgccc gagcagcgag
      601 ggcttccagt tttgcctcaa cggcgcgtgc tcgagcctgt gcggcaccga cgactacaag
      661 aagtacgacc tcgactcgaa cgacgacttc tgcgccgagg gccagacctg catccccttc
      721 gacgccgccg gcctcgaccc gctggtgctc gagaccttcg gcgtcggcag caacagccag
      781 ggcggcttca acatcgccat gttgccgatc ggcgccggca tgtgtggcgc gcgctgcgac
      841 gccgagggcg cccgcccgtg ccccgccggt caatactgca ccggcgccct gtgcatgccc
      901 ggctgcgacc aggagaccgc gaccccgtgc gacgccggca ccaagtgcat cgccctggcc
      961 ggcttctcga gctgcctggt cgcctgtgac ccgaacgtga ccggctcctg cggcgagggc
     1021 gagatctgcg tcccgggcct cgatgtctgc cagccgacct gcctcggccc cgacaaggtc
     1081 gagtgcccgg acagcttcga gtgcgacgcg accctggcga tctgcatccc gatcggcggc
     1141 gacgagagca ccggcggcga cacgaccgac ggcgcgacca ccggcggcgc gaccaccgac
     1201 ggcgcgacca cctgagggaa ggacgaacac gatgacgagc cttcgcacct ccttcctggt
     1261 cgccgccctc agcctgagca ccgcctgcgc cggcctcgag ccccagccgg tgcgcctcga
     1321 cgcccgcctc gccagcctca acatcggcaa catgggcgtg cgcaacagct acgagctgcg
     1381 caacgcgagc ccgatcgccg gccgcgcgac cgagacggtg ccggtcgacg actctcgccc
     1441 gcgcgagcgc cgaccgatca cgccggtgct gttctggctc ggcatcggcc tgaccgcggt
     1501 cggcggcgtc agcacgatcg
//
LOCUS       DASWNJ010001874         1876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_455507, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001874 DASWNJ010000000
VERSION     DASWNJ010001874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455507"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1876
                     /locus_tag="VGB85_31255"
     CDS             <1..>1876
                     /locus_tag="VGB85_31255"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0138618.1"
                     /translation="GLLGRDDELAALDLALADARAGQAAVVLLHAATAGLGRSAVIDH
                     FVAARERDAIVVLSGRCSERESLPYKGVDGLIDALARELHRRADPARLCPPHIHTLAR
                     LFPVLRRVPAIARATPGPDDGGLAPHELRQLAFEALRQLLARLAGEAPLVLVLDDLHH
                     GDHDSAALLRELLAPPDPPPLLLIASHRGDTADSACVRALASLDLRLRRLALGPLAPA
                     DATALAQSLLAGSSRAGEAAAIAREAAGNPLFIVELCHFVRGAEAVDPLAQASSFDAL
                     LRRRIAGLDLASRALLEVVAVAAEPIDLALVAAVAGHPGDPQPTLTALRREHLLRSHR
                     VDGRTTVEVFHDRVREALLADLAPTRAREIHRDLADALERSEAPDIERLRVHLAAAGE
                     TTRAAAYTLRAAERASTALAFERAAALYRAAREHAAPEDRPRLDILLGDTLASDGRGV
                     EAADAYLRAADALGGTQALELLRRAGFERLRSGHIDEGIAVLQKVLASVDMRLSGSRG
                     RALLSLAGHRVALALRGLRFHERPEAEIPPELLRRADVAWSIGASGLGMVDDIAAGEF
                     QSLALRLALRAGEPMRICRALAGEVAFSAVGGTASLRRTTRLLHTARTLAERLDQPQA
                     R"
BASE COUNT          199 a          824 c          629 g          224 t
ORIGIN      
        1 ggcctgctcg gccgcgacga cgagctcgcc gccctcgacc tcgccctcgc cgacgcgcgc
       61 gcgggccagg ccgccgtcgt cctgctgcac gccgcgaccg ccggcctcgg ccgcagcgcc
      121 gtgatcgacc acttcgtcgc cgcccgcgag cgcgacgcga tcgtcgtgct gtccggccgc
      181 tgcagcgagc gcgagagcct gccctacaag ggcgtcgacg gcctgatcga cgccctcgcc
      241 cgcgagctgc acaggcgcgc cgacccggcc cgcctgtgcc cgccgcacat ccacacgctc
      301 gcgcgcctgt tcccggtgct gcgccgcgtc cccgcgatcg cccgcgccac gcccggaccc
      361 gacgacggcg gcctcgcccc gcacgaactg cgccagctcg ccttcgaggc gctgcgccag
      421 ctgctcgcgc gcctcgccgg cgaggccccg ctggtgctcg tgctcgacga cctccaccac
      481 ggcgaccacg acagcgccgc gctgctgcgc gagctgctcg ccccgcccga cccgccgccg
      541 ctgctgctga tcgccagtca ccgcggtgac acggccgaca gcgcgtgcgt gcgcgcgctc
      601 gcgagcctcg acctgcgcct gcgccggctc gcgctcggcc cgctcgcgcc cgccgacgcg
      661 accgccctcg cgcagtcgct gctcgccggc tcgtcgcgcg ccggcgaggc cgcggcgatc
      721 gcccgcgagg ccgccggcaa cccgctgttc atcgtcgagc tgtgtcactt cgtgcgcggc
      781 gccgaggcgg tcgatccgct ggcgcaggcc tcgagcttcg acgccctgct gcgccgccgg
      841 atcgccggcc tcgacctggc gagccgcgcc ctgctcgagg tcgtcgccgt cgccgcggag
      901 ccgatcgacc tcgcgctcgt cgccgccgtc gccggtcacc ctggcgaccc acagccgacc
      961 ctgaccgcgc tgcgccgcga acacctgctg cgcagtcatc gggtcgacgg acggaccacc
     1021 gtcgaggtgt ttcacgaccg cgtgcgcgag gcgctgctcg ccgacctcgc gccgacccgc
     1081 gcccgcgaga tccaccgcga cctcgccgac gcgctcgagc gcagcgaggc ccccgacatc
     1141 gagcgcctgc gcgtccacct cgccgccgcc ggcgagacca cccgcgccgc cgcctacacc
     1201 ctgcgcgcgg cggagcgcgc cagcacggcc ctcgcgttcg agcgcgccgc ggccctctac
     1261 cgcgccgccc gcgagcacgc ggcccccgag gaccgaccgc gcctcgacat cctgctcggc
     1321 gacaccctcg ccagcgacgg ccgcggcgtc gaggcggccg acgcctacct gcgggccgcc
     1381 gacgcgctcg gcggcaccca ggcgctagag ttactgcgcc gcgccggctt cgagcgcctg
     1441 cgcagcggtc acatcgacga gggcatcgcg gtgctgcaaa aggtgctcgc cagcgtcgac
     1501 atgcgtctgt ctgggagccg cgggcgggcc ctgctgtcgc tcgccggtca ccgcgtcgcc
     1561 ctcgcgctcc gcggcctgcg ctttcacgag cgccccgagg ccgagatccc gccggagtta
     1621 ctacgccgcg ccgacgtcgc ctggtcgatc ggcgcctccg gcctcggcat ggtcgacgac
     1681 atcgccgccg gcgagttcca gagcctcgcg ctgcgcctgg cccttcgggc aggtgagccg
     1741 atgcggatct gtcgcgccct cgccggtgag gtcgccttct cggccgtcgg cgggaccgcc
     1801 agcctgcgcc gcaccacgcg tctgctgcac accgcccgca ccctcgccga gcgcctcgac
     1861 cagccgcagg cgcgcg
//
LOCUS       DASWNJ010001875         2771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_173115, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001875 DASWNJ010000000
VERSION     DASWNJ010001875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173115"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VGB85_31260"
     CDS             <1..809
                     /locus_tag="VGB85_31260"
                     /inference="COORDINATES: protein motif:HMM:NF018427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138619.1"
                     /translation="NSSPGGGGSNELRFEDQAGAEEVYLHAQMTFNAVVGADMSLSVG
                     GSESDSISANRTTTVGANDTTTVGANHTTTVGANITSTAGANHAASAAATQSFSAGAS
                     QSLSAGSSQSLSAGSSQSLSAGADQSISVGGNRTVSVTGNETVSVTGTQSTSVTGAHS
                     LTTSAASTVDIGAALTHSIGADYTLSITGNNTVTAVEYSLTASKITLSGGGSIITMEG
                     GKISISSSGPLDLSGCGPITIQGPNIDIKGGPINIEGADVTVKGGTIKLN"
     gene            819..1871
                     /locus_tag="VGB85_31265"
     CDS             819..1871
                     /locus_tag="VGB85_31265"
                     /inference="COORDINATES: protein motif:HMM:NF021456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HEY0138620.1"
                     /translation="MELVNYTPVPAQLRVSTIEGRDDARHGIIAAKATYTVASDGTAT
                     LETQTPYPLFEVDQPTPFGLLPADSVPRRDRALEVIVLGAAHGQGRARMTVELEVGRH
                     RQSLLVTGDRYWTGQRRGAEISEPLPIDRMPLNWDRAHGGSAECWIDEHSILDLEHPM
                     NRYGRGFDAGKLAQDLGKAFRPPAGYPRLAPDYRRWLPNIEDPRHPIDGWNDDPRPYC
                     WATLPTDIGAHVQQAHDRVRDGEPMTPDEMLQVVYHRAHPDWVLPVPPSEASVTLRGM
                     TTRGTWSFRLPRLQVFADYELGERTGTRELEPHLLMLLPEESRFYLVYRHFFTMEVRR
                     GMRRSFRLRLAEGWNV"
     gene            1868..>2771
                     /locus_tag="VGB85_31270"
     CDS             1868..>2771
                     /locus_tag="VGB85_31270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138621.1"
                     /translation="MTKVTIQRDLCLNLIVPLTAAHDFIPLPPFPPIPPPLPGLPYSL
                     AACALEMPVPAWWPPGGALGSHKFTSTVFHNGMQICLDGHDCGKFIPHIQIMPAFNNM
                     LTFIHIPFSSRKANFSASTVLMNGKPTAGMTMIAWPPTPMTYCADPIGLPLGDAPTSH
                     FNTVEIHISLADWLAGAFVIAAGMVLDYVMFKRGGGTRKFGQDAGKDIAKSRAGKSAA
                     SLIASRIKSKVLVPFSKKWAIKQGIGVATGLARIGLTGQGSVGVTAPFGGGFGSLGGS
                     VSKTDGGWNPGAAGSVGTVSGSVDT"
BASE COUNT          459 a         1099 c          827 g          386 t
ORIGIN      
        1 ccaactcgtc gcccggcggc ggcggctcca acgagctgcg cttcgaggac caggccggcg
       61 ccgaggaggt ctacctccac gcgcagatga ccttcaacgc cgtcgtcggc gccgacatgt
      121 ccctcagcgt cggcggcagc gagtccgact cgatctccgc caaccgcacc accaccgtcg
      181 gcgccaacga caccaccacc gtcggcgcca accacacgac caccgtcggc gccaacatca
      241 cctcgaccgc cggtgccaac cacgccgcct ccgccgcggc cacccagtcg ttcagcgccg
      301 gcgccagcca gtcgctctcc gccggctcgt cgcagtccct ctccgccggc agcagtcagt
      361 cgctctccgc cggcgccgac cagtcgatct ccgtcggcgg caaccggacc gtctccgtca
      421 ccggcaacga gaccgtctcc gtcaccggca cgcagtcgac ctcggtgacc ggcgcccaca
      481 gcctgaccac ctccgccgcc tcgaccgtcg acatcggcgc cgccctgacc cacagcatcg
      541 gcgccgacta caccctgtcg atcaccggca acaacaccgt caccgccgtc gagtactcgc
      601 tgaccgccag caagatcacc ctcagcggtg gcggtagcat catcaccatg gaaggcggaa
      661 agatctcgat cagctcgtcg ggcccgctcg acctcagcgg ctgcggcccg atcacgatcc
      721 agggcccgaa catcgacatc aagggcggcc cgatcaacat cgagggcgcc gacgtcaccg
      781 tcaagggcgg cacgatcaag ctcaattgag ccagctgcat ggagctcgtc aactacacgc
      841 cggtcccggc gcagctgcgc gtctcgacga tcgaaggccg ggacgacgcg cgccacggca
      901 tcatcgccgc caaggcgacc tacacggtcg ccagcgacgg caccgcgacc ctcgagaccc
      961 agaccccgta cccgctgttc gaggtcgacc agccgacccc gttcggcctg ctgcccgccg
     1021 acagcgtgcc gcgccgcgac cgtgccctcg aggtgatcgt cctcggggcc gcgcacgggc
     1081 aagggcgcgc gcgcatgacc gtcgagctcg aggtcggtcg tcaccgtcag agcctgctgg
     1141 tcaccggtga ccgctactgg accggccagc gccgcggcgc cgagatctcc gagccgctgc
     1201 cgatcgaccg catgccgctc aactgggacc gcgcccacgg cggctcggcc gagtgctgga
     1261 tcgacgagca cagcatcctc gacctcgagc acccgatgaa caggtacggt cggggcttcg
     1321 acgccggcaa gctcgcgcag gacctcggca aggcctttcg cccgcccgcc ggttacccca
     1381 ggctcgcgcc cgactaccgc cgctggctgc ccaacatcga ggacccgcgc cacccgatcg
     1441 acggctggaa cgacgacccg cgaccgtact gctgggccac gctgccgacc gacatcggcg
     1501 cccacgttca acaggcgcac gaccgcgtcc gcgacggcga gccgatgacg cccgacgaga
     1561 tgctgcaggt cgtgtaccac cgcgcccacc ccgactgggt gctcccggtc ccgccgtccg
     1621 aggcgagcgt cacgctgcgt ggcatgacca cgcgcggcac ctggagcttt cgtctgccgc
     1681 gcctgcaggt cttcgccgac tacgagctcg gcgagcgcac cggcacccgc gagctcgaac
     1741 cacacctcct gatgttgttg cccgaggagt cgcggttcta cctcgtctat cggcattttt
     1801 tcacgatgga ggtccgccgc ggcatgcgac ggtcgtttcg cctccggctc gccgaagggt
     1861 ggaacgtatg accaaagtga cgatccagcg cgacttgtgc ctcaacttga tcgtcccgct
     1921 gacggcggcg cacgacttca tcccgctgcc gccgtttccc ccgatccctc cgccgctgcc
     1981 cggcctgccg tactcgctgg ccgcctgcgc cctcgagatg cctgtgcccg cctggtggcc
     2041 gcccggcggc gcgctcggct cgcacaagtt caccagcacc gtgttccaca acggcatgca
     2101 gatctgcctc gacggtcacg actgcggcaa gttcatcccg cacatccaga tcatgccggc
     2161 gttcaacaac atgttgacgt ttatccacat cccgttctcc tcgcgcaagg cgaacttcag
     2221 cgcgagcacc gtgctgatga acggtaaacc aacggccggc atgacgatga tcgcctggcc
     2281 gccgacgccg atgacctact gcgccgaccc gatcggcttg ccgctcgggg acgcgccgac
     2341 cagccacttc aacaccgtcg agatccacat cagcctcgcc gactggctcg ccggcgcctt
     2401 cgtgatcgcc gccggcatgg tgctcgacta cgtcatgttc aagcgcggcg gcggcacccg
     2461 caagttcggt caggacgccg gcaaggacat cgccaagagc cgcgccggca agagcgccgc
     2521 ctcgctgatc gcctcccgca tcaagtccaa ggtcctggtc ccgttcagca agaaatgggc
     2581 gatcaagcag ggcatcggcg tcgccaccgg cctcgcccgc atcggcctca ccggccaggg
     2641 ctccgtcggc gtcaccgccc cgttcggcgg cggcttcggc tcactcggcg gctcggtcag
     2701 caagaccgac ggcggctgga acccgggcgc cgccggcagc gtcggaactg tcagcggcag
     2761 cgtcgacacc a
//
LOCUS       DASWNJ010001876         1991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_104564, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001876 DASWNJ010000000
VERSION     DASWNJ010001876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104564"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VGB85_31275"
     CDS             <1..415
                     /locus_tag="VGB85_31275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HEY0138622.1"
                     /translation="GTGLPAHGDYSYGAYQSNWALWPVGELLRLDGSGERCVVLGATS
                     LIDGSTAMSEPRCAEIAAPREATLMPEFLPADLEGSGMGECLSPPVYEHSGEPYLPYD
                     GGETTVEQGGCRVAGQGPWGLLVLGLVWRRRARRG"
     gene            417..>1991
                     /locus_tag="VGB85_31280"
     CDS             417..>1991
                     /locus_tag="VGB85_31280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138623.1"
                     /translation="MIAKWRGCTIALLVGCTASEPKSVPIEKPMLVEKPVLVEKPVLV
                     EVSEVRPPSTTWLAQWAGQDFDEVNGLAITPEGDTLAVGGVAGAVVLSSAGFAPTTVM
                     APLLKPNRSGLLVKYGQYGDLRFAVPFALTDDADAVAATPDGGAVVVGSVKKPLVETD
                     GPGLYEREEPDGFVARFDAAGKPVWRRVLAGPSTQTITEVAAHPQGGYVVAGVFHGDS
                     RFDGDARSMGVGMAPKNEEELGISYDVFVARLAEDGEVMWIGELGSEEQEVVGGVAVG
                     ADGTVVLTGECRLRTRVRGGGRTQTITCGDLSIGGFLATWRADGELEWATRIPGPERD
                     TQSPMGVTVLADGSIVAVGLFKKGIGGGALPLLSNGDGLVDGFVVAYTAKGQPAWLRH
                     LRGPSVELFQAAVAAPGGGVWVLGNGSADMEFGDGTKYEGRVPRAGNDALLLRYDAGG
                     ELVYAGLYGGKPAPRESSPTVADADMSEVRPYALAVAADGSLRIGGSFDGGLALAGDG
                     APKLRASADLDGFVIAV"
BASE COUNT          274 a          581 c          849 g          287 t
ORIGIN      
        1 cggcacgggt ttgccggcgc acggcgacta ttcgtacggt gcataccagt cgaactgggc
       61 gctgtggcca gtgggcgagc tgctgcgcct cgacggctcg ggcgagcgct gcgtggtgct
      121 cggcgcgacg agcttgatcg acggcagcac ggcgatgtcg gagccgcgtt gcgccgagat
      181 cgcggcgccg cgcgaagcga cgctgatgcc cgagttcttg cccgcggacc tcgagggctc
      241 cgggatgggc gagtgcctgt cgcccccggt gtacgagcac agcggcgagc cctatctgcc
      301 gtatgacggt ggggagacga cggtcgagca ggggggctgc cgcgtggccg gccaaggtcc
      361 gtgggggctg ctggtgctcg ggctggtgtg gcggcgcagg gctcggcggg ggtgacatga
      421 tcgcgaaatg gcgaggttgt acgatcgcgc tgctcgtcgg ttgcacggcg agcgagccga
      481 agtcggtgcc catcgagaag ccgatgctcg tcgagaagcc ggtgctcgtc gagaagccgg
      541 tgctcgtcga ggtgtcggag gtgaggccgc cgtcgacgac gtggctcgcg cagtgggcgg
      601 ggcaggactt cgacgaggtc aacgggcttg cgatcacgcc ggagggcgac acgctcgcgg
      661 tcggcggggt cgcgggggcg gtcgtattga gcagcgcggg ctttgcaccg accacggtga
      721 tggccccatt attaaaaccg aaccgatcgg ggttgctggt caagtacggg cagtatggcg
      781 atctgcggtt cgcggtcccg ttcgcgctca cggatgacgc ggacgccgtg gcggcgacgc
      841 cggacggcgg ggcagtggtc gtcggttcgg tgaagaagcc gctggtcgag accgacggcc
      901 cggggctgta cgagcgcgag gagccggacg gctttgtcgc ccgcttcgac gcggcgggca
      961 agccggtgtg gcgcagggtg ctggcgggcc cgagcacgca gacgatcacc gaggtcgcgg
     1021 cgcatccgca gggcggctac gtggtcgcgg gggtgtttca cggcgacagc cgcttcgacg
     1081 gcgatgcgcg cagcatgggc gtcggcatgg cgccgaagaa tgaggaggag ctggggatct
     1141 cgtacgacgt gttcgtcgcg cggctcgccg aggacggcga ggtgatgtgg atcggcgagc
     1201 tcggcagcga ggagcaggag gtcgtcggcg gggtggcggt cggggccgac ggcacggtgg
     1261 tgctgacggg cgagtgccgg ctgcgcacgc gggtgcgcgg aggcgggcgc acgcagacga
     1321 tcacctgcgg ggacctgagc atcggtggtt tcctggcgac gtggcgggcc gacggcgagc
     1381 tcgagtgggc gacgcggatc cccggacccg agcgcgacac gcagagcccg atgggcgtca
     1441 cggtgctggc cgacggcagc atcgtcgcgg tcggactgtt taaaaagggg atcggtggcg
     1501 gagcgctgcc gctgctgagc aatggcgacg ggctggtcga cggcttcgtg gtcgcgtaca
     1561 cggcgaaggg gcagcctgca tggttgaggc acctgcgcgg gccgtcggtc gagctgttcc
     1621 aggcggccgt ggcggcgccc gggggcgggg tgtgggtgct cggcaacggc tcggccgaca
     1681 tggaattcgg cgacggaaca aagtacgagg gccgggtccc gcgcgccggc aacgacgcgc
     1741 tgctgctgcg ctacgacgcc gggggcgagc tggtatacgc ggggctgtac ggcggcaagc
     1801 cggcgccgcg ggagagcagc ccgacggtcg cggacgcgga tatgtcggag gtccggccgt
     1861 acgcgctggc ggtcgcggcg gacggctcgc tgcggatcgg tgggagcttc gacggcgggc
     1921 tcgcgctggc cggtgacggc gcgccgaagc tgcgggccag cgccgacctc gacggcttcg
     1981 tgatcgccgt g
//
LOCUS       DASWNJ010001877         4818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_492680, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001877 DASWNJ010000000
VERSION     DASWNJ010001877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492680"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VGB85_31285"
     CDS             <1..268
                     /locus_tag="VGB85_31285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138624.1"
                     /translation="VCVSDEAPTCADYCATYLANCQSTSAYKDEADCHAQCAGWYPGA
                     AGQVSGDTIGCRAYHASVAPGDPAVHCPHAGPTGADVCVPAVGE"
     gene            complement(312..1991)
                     /locus_tag="VGB85_31290"
     CDS             complement(312..1991)
                     /locus_tag="VGB85_31290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138625.1"
                     /translation="MRRARRALLGGSLALFSACGGGGSDREPGATAEGTTDATGATGV
                     TGTGSSPTGTTPGTDTGDVPTTGATEDTDVTGDTGNTVGTNDTGNTADTGDTDTGGPG
                     QLGGQFLRGINLGYRNPAWGDPDMGWLAGQAGSTSARLSLHEGHLDKWGYEIELGDME
                     SYASSGLGHHIAFLIAPIAAHSTAPDGTPDWELAHYIPKNLHTPAIVDGKVNPENYWA
                     AYVYKTVDTYKDHVDTWEVWNEPDWVADYNFTLTWDEQPPTAAQLVRFGGSIYDYVRM
                     LRVTMEAAKLADPEAKIALGGIGYPSFLAALLRYTDDPQAGAVTPEYPETGAAYFDVL
                     NYHYYPLWTPGNSDAGVDGFFKLQAEFAGKMQAAGAAPRPFSVTEVGAPRSSYDGGPG
                     GVEYARNFYVKVMTRAQTEGFLRVDWFILSDAVHDSAFNEMGLYEDVVAVAKKEDAKR
                     TPTGVAARTHGMLLGEALADPGATAAIGAGPDVQAHAFRLPDDRQVLVLWAYAAGADE
                     SAQAHLDLATDRSFDLHAWDFSETGSKTPLAPQGGVIALDLTASPVILVEP"
     gene            complement(2117..3370)
                     /locus_tag="VGB85_31295"
     CDS             complement(2117..3370)
                     /locus_tag="VGB85_31295"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY0138626.1"
                     /translation="MTMSTRPKLRTLLLSIGLAVTAGCGDSAIGGSEGGAATSSSTGS
                     DAPGLTTGTGAPGSSSVPTSSSGEDSGGEPGTTGTSESSESSETGESSGTTGTSGVTG
                     DAGTTGEVSEGGGESSGTGGSESGSESGEESGEESGGAPDPVIPGPKVKFMSFNIRVG
                     TAADGEDAWDKRKGLVYQVFKDQDADFIGVQEALTFQLVAIDNNVKGYERIGVATIDG
                     KAKGPVNAIYFRKSRFERKKSATFWLSNTPDVPGSQTWGNKFPRAVTWGRFAEKDTGY
                     TFYVFNTHFDHVSQNSREKSAVLLAKRIAERGSPNDPFVAMGDLNSGESNLVVRFLKG
                     DAKIDTKANEVPMRDSFRVAHPDANNVGTAHGFNGGTGGNKIDYIFVPPKQKVNGAAI
                     DHFNVNGRYPSDHFPITGTVTFADK"
     gene            complement(3432..>4818)
                     /locus_tag="VGB85_31300"
     CDS             complement(3432..>4818)
                     /locus_tag="VGB85_31300"
                     /inference="COORDINATES: protein motif:HMM:NF018000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138627.1"
                     /translation="DEQLRPRLRGIGRTRVVVRAGDPTDPGAIDRVAPEHARSIVVLA
                     PEGAHDDTQVMRTLLSIGRTAPAPGRKQHVVTEIHDPRNIDVVRLTGDRRIEVLEVGD
                     LIAKIAVQTCLQSGLSFVYEELLGFAGDEIYFVDAAPLVGHSFARALHQFEDCTLLGL
                     CGVDGKVQLKPAMDRVIVAGERLILIAADDDRCVVRPWSGAVDEASIVSTPASVRRPE
                     RTLILGWNARIPAIVRGIDAYVTPGSTLTVVCSDPAAEPALAGLRPGLRNMELGHRPG
                     DISDRRILDALDLRTWDHVMVVPDDRITAATEADAQVLVALLHLRDLVEGLPRRFSII
                     TEMRDVRSRDLAEVAQADDFIISDRFIGLLLAQVAENADLAAVFADLFDPDGAEIYLR
                     PATDYVVAGREVDGYTLVEAGARRGEVVLGVRLAPRATPGPLVDVVVNPRKTTRLTLT
                     PEDRVIVLADA"
BASE COUNT          666 a         1726 c         1656 g          770 t
ORIGIN      
        1 cgtgtgcgtg tccgacgagg ccccgacctg cgccgactac tgcgccactt atctcgccaa
       61 ctgccagagc accagcgcct acaaggatga ggccgactgc cacgcgcagt gcgcaggctg
      121 gtatccaggg gccgctggtc aggtctcggg cgataccatc ggctgccgcg cctaccacgc
      181 cagtgtcgcg cccggcgatc ccgccgtgca ttgcccacat gccggcccca cgggcgccga
      241 cgtgtgcgtg ccggccgtcg gcgagtgacg cacgcgcagg cgtcggcccc cgagatccga
      301 cgcgcccgcc ctcacggctc gacgaggatc accggcgagg ccgtcaggtc gagcgcgatg
      361 accccgccct gcggcgccag cggggtctta ctgccggtct cgctgaagtc ccaggcgtgc
      421 aggtcgaagc tgcggtcggt cgccaggtcg aggtgcgcct gcgccgactc gtcggccccg
      481 gcggcgtacg cccacagcac gagcacctgg cggtcgtccg gcaggcgaaa cgcgtgcgcc
      541 tgcacgtccg ggccggcgcc gatcgccgcg gtcgcgccgg ggtcggcgag cgcctcgccg
      601 agcagcatgc cgtgcgtgcg cgcggcgacg ccggtcggcg tgcgcttggc gtcctccttc
      661 ttcgccacgg cgacgacgtc ctcgtagagc cccatctcgt tgaacgcgct gtcgtgcacg
      721 gcgtcgctga ggatgaacca gtcgacgcgc aggaagccct cggtctgcgc gcgcgtcatc
      781 accttgacgt agaagttgcg cgcatactcg acgccgcccg gtccgccgtc gtaagacgag
      841 cgcggggcgc cgacctcggt gacgctaaac ggccgcggcg ccgcgcctgc ggcctgcatc
      901 ttgccggcga attccgcctg gagcttgaaa aatccgtcga ccccggcgtc cgagttgccc
      961 ggcgtccaca acggataata gtggtagttg agcacatcga agtaggccgc gccggtctcc
     1021 gggtactcgg gggtcaccgc gcccgcctgc ggatcgtcgg tgtaacgcag cagcgccgcg
     1081 aggaagctcg ggtagccgat cccgccgagg gcgatcttcg cctccgggtc cgcgagcttg
     1141 gccgcctcca tcgtcacccg aagcatgcgc acataatcgt agatcgaccc gccgaagcgg
     1201 accagctgcg ccgcggtcgg cggctgctcg tcccaggtca gcgtgaagtt gtagtccgcg
     1261 acccagtcgg gctcgttcca cacctcccag gtgtcgacgt gatctttgta agtgtcgacc
     1321 gtcttgtaga catacgccgc ccagtagttc tcggggttga ccttgccgtc gacgatcgcc
     1381 ggcgtgtgca ggttcttggg gatgtaatgc gcgagctccc agtccggcgt gccgtcgggc
     1441 gcggtcgagt gcgccgcgat cggtgcgatc aaaaacgcga tgtggtgccc cagcccgctg
     1501 ctcgcgtacg actccatgtc gccgagctcg atctcgtagc cccacttgtc gaggtgcccc
     1561 tcgtgcaacg acagccgcgc gctcgtgctg ccggcctggc ccgccagcca gcccatgtcc
     1621 gggtcgcccc acgcggggtt gcgatacccg aggttgatcc cgcgcaggaa ctgcccgccg
     1681 agctgcccgg gtccgccggt atcggtgtcc cccgtatcgg cggtgttacc ggtgtcgttc
     1741 gtccccacgg tattgcccgt gtccccggtg acgtcggtat cctcggtggc gcccgtcgtc
     1801 ggcacatcac cggtgtcggt ccccggggtc gtgccggtcg gcgaggatcc cgtcccggtg
     1861 acgccggtcg ccccggtcgc gtcggtggtc ccctccgcgg tcgcgcccgg ctccctgtcc
     1921 gagccaccgc cgccgcacgc ggagaagaga gcgagagaac caccgaggag ggcccgtcga
     1981 gcgcgtcgca tcggcgcatc gaaacacacg cagggccgcc tgtcgccggt cctcgacgcg
     2041 ggacatcgcg ggggcagcgg tcctcggccc ggcgcgcgcg ctcgctatcg ggcgccgcgg
     2101 tgcagttgcg agccggctac ttgtccgcga aggtcaccgt gccggtgatc gggaagtggt
     2161 cggagggata gcggccgttg acgttgaagt gatcgatcgc cgcgccgttc accttttgct
     2221 tcggcggcac gaagatgtag tcgatcttgt tgccgccggt gccgccgttg aagccgtgcg
     2281 cggtgccgac attgttggcg tcggggtgcg cgacgcggaa gctgtcgcgc atcgggacct
     2341 cgttggcctt ggtgtcgatc ttggcgtcgc ccttcaggaa gcgcacgacg aggttgctct
     2401 cgccggagtt gaggtcgccc atcgccacga acgggtcatt gggcgatcca cgctccgcga
     2461 tccgcttcgc cagcaggacg gcgctcttct cccgcgaatt ctgcgacacg tgatcgaagt
     2521 gggtgttgaa gacatagaaa gtatatccgg tgtccttctc ggcgaagcgg ccccaggtga
     2581 ccgcgcgcgg gaacttgttg ccccacgtct gcgatcccgg cacatccggc gtgttcgaca
     2641 gccaaaaggt cgccgacttc ttccgctcga agcgagactt gcgaaaatag atcgcgttga
     2701 cgggcccctt ggccttgcca tcgatcgtcg cgacgccgat ccgctcgtag cccttgacgt
     2761 tgttgtcgat cgccacgagc tgaaaggtca gcgcctcctg gactccgatg aagtcggcgt
     2821 cctgatcctt gaacacctga taaaccaagc ccttgcgctt gtcccacgcg tcctcgccgt
     2881 ccgccgcggt gccgacgcga atattgaagc tcatgaactt gactttgggc ccggggatca
     2941 ccggatccgg cgcaccaccg ctctcctcgc cgctctcctc gccgctttcc gaaccgcttt
     3001 ccgaaccgcc ggtccccgag ctctcgccgc cgccctccga cacctcgccc gtggtgcccg
     3061 cgtcgccggt gactccgctg gtcccggtcg tcccgctgct ctcgccggtc tcgctgctct
     3121 cgctgctctc gctggtcccg gtcgtcccgg gttcgccgcc gctgtcctcg ccgctgctcg
     3181 aggtcggcac gctcgaactg cccggggcgc ccgtgccggt ggtcaggccc ggcgcatcgc
     3241 tgccggtgct cgagctcgtc gccgcgccgc cctccgagcc gccgatcgcc gagtctccgc
     3301 agcccgcggt caccgccagg ccgatcgaga gcaggagcgt tcgcagcttc ggccgggtgg
     3361 acatggtcat gcatccaacc tgcccgccgg gctcgccacg cgtcaagcgt gatcgggcgc
     3421 gcacgggccc gtcaagcgtc ggcgagcacg atgacgcggt cctcgggcgt cagcgtcagg
     3481 cgtgtcgtct tgcgtgggtt gaccaccaca tcgacgagcg ggcccggggt cgcgcgcggg
     3541 gcgaggcgca cgccgagcac gacctccccg cgacgggcgc cggcctcgac gagcgtataa
     3601 ccatcgacct cgcggcccgc gaccacgtag tcggtcgccg gacggagata gatctcggcg
     3661 ccatcgggat cgaagaggtc ggcgaacacc gcggcgaggt cggcgttctc ggcgacctgc
     3721 gcgagcagca ggccgatgaa gcggtcgctg atgatgaagt cgtcggcctg cgcgacctcg
     3781 gcgaggtcgc ggctccgcac gtcacgcatc tcggtgatga tcgagaaccg ccgcggcagg
     3841 ccctccacga ggtcgcgcag gtgcagcaac gcgaccagca cctgcgcgtc ggcctcggtc
     3901 gccgcggtga tgcggtcatc gggcacgacc atcacgtgat cccaggtgcg caggtcgagc
     3961 gcgtcgagga tccggcggtc gctgatgtcg ccggggcgat gcccgagctc catgttgcgc
     4021 agccccggcc tcagcccggc gagcgcgggc tcggccgcgg gatcggagca gacgaccgtc
     4081 aacgtcgagc ccggcgtcac gtaggcatcg atgccacgca cgatcgccgg gatgcgcgcg
     4141 ttccagccga ggatcagcgt gcgctccggg cggcggaccg acgcgggggt cgagacgatg
     4201 ctcgcctcgt cgaccgcgcc cgaccacggg cgcacgacgc agcggtcgtc gtcggcggcg
     4261 atcaagatca ggcgctcgcc ggccacgatg acgcggtcca tcgccggctt caattgcacc
     4321 ttgccgtcga ccccgcacag gccgagcagc gtgcaatcct cgaactgatg cagcgcgcgc
     4381 gcgaagctgt gcccgacgag cggggccgcg tcgacgaagt agatctcgtc gcccgcgaag
     4441 ccgagcagct cctcgtagac gaacgacagg ccggactgca ggcaggtctg gacggcgatc
     4501 ttggcgatca ggtcaccgac ctcgagcacc tcgatgcgcc ggtcgccggt caggcgcacg
     4561 acgtcgatgt tgcgggggtc gtggatctcg gtgacgacgt gctgcttgcg ccccggggcc
     4621 ggcgccgtgc ggccgatcga cagcagcgtg cgcatgacct gggtgtcgtc gtgcgcgccc
     4681 tcgggggcga gcacgacgat cgaccgcgcg tgctccggcg cgacccggtc gatcgcgccg
     4741 gggtcggtcg gatcgccggc gcggacgacc acgcgcgtgc gaccgatgcc gcgcagccgc
     4801 ggccgcagct gctcgtcc
//
LOCUS       DASWNJ010001878         1986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_527283, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001878 DASWNJ010000000
VERSION     DASWNJ010001878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527283"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1521)
                     /locus_tag="VGB85_31305"
     CDS             complement(<1..1521)
                     /locus_tag="VGB85_31305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEY0138628.1"
                     /translation="MCPAWSSRLAFIAPLVLAACPNEGPPGTGEDSQSETAPMTTTGT
                     PDTTTSVTCEIGMLGCPCTGGGACDPGLVCTAEKVCQSAQDLTTGSDTSVDPTMTAGT
                     STTTDSEGGQTSTTDDTEPPGPECTPTGDKLESAECLAKDPNRPFCEVDTCVGCGALK
                     ADACTDATDGGRPICLDDGRCVQCDTSDAVVQGQCEADKPHCNLETNTCEGCFEHSEC
                     PQTACEIAARKCFPANQKIYVRQGPTPGSPCSTKPGMGGTMELPYCDFETATLGVQQN
                     GFSSGYTFHLMGNDDAPGDPGAVVITGGDAKVAYAFTHDFGGLVDKHTRFKGIGPLIL
                     VPKNVTLYLVNWGLLIDKPQGDTSRGIDCQEGGSVWLDDSRVLYARGPGIRGTGCDIH
                     LRRTAVSLGATEAIDMTGGSLHAVNSFIAGNANIVGKGGGGLHLRDGATVDVVYSTIA
                     DNSNEPSQMPGQFGDSIHCDNAASVKIRNSIIVRKPANNNFSIVCPEGQVSVDWSVV"
     gene            complement(1801..>1986)
                     /locus_tag="VGB85_31310"
     CDS             complement(1801..>1986)
                     /locus_tag="VGB85_31310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138629.1"
                     /translation="KAGLGNVFSGYADGVYQAKIGGSLEDVALWLTGDPAVDFDENLR
                     PIVNETLDYAGADRPVL"
BASE COUNT          270 a          662 c          720 g          334 t
ORIGIN      
        1 gacgaccgac cagtcgacgc tcacctgacc ctcgggacag acgatgctga agttgttgtt
       61 ggccggcttg cggacgatga tcgagttgcg gatcttgacc gaggccgcgt tgtcgcagtg
      121 gatcgagtcg ccgaactggc cgggcatctg cgacggctcg ttgctgttgt cggcgatcgt
      181 gctgtagacc acgtcgacgg tcgcgccgtc gcgcaggtgc aggccgccgc cgcccttgcc
      241 gacgatgttg gcgttgccgg cgatgaagct gttgaccgcg tgcagcgagc cgccggtcat
      301 gtcgatcgcc tcggtcgcgc cgagggagac ggccgtgcgg cgcaggtgga tgtcgcagcc
      361 ggtgccgcgg atgccggggc cgcgggcgta cagcacgcgc gagtcgtcga gccacaccga
      421 gccgccctcc tggcagtcga tgccgcgcga ggtgtcaccc tgcggcttgt cgatcaggag
      481 cccccagttc acgaggtaca gcgtgacgtt cttgggcacc aggatcagcg ggccgatacc
      541 cttgaagcgg gtgtgtttgt cgaccaggcc gccgaagtcg tgggtgaagg cgtacgcgac
      601 cttggcgtcg ccgccggtga tgacgaccgc gccgggatca ccaggcgcgt cgtcgttgcc
      661 catcaggtgg aaggtgtagc cggacgagaa gccgttttgc tggacgccga gcgtcgcggt
      721 ctcgaagtcg cagtacggga gctccatcgt gccgcccatg cccggcttgg tcgagcacgg
      781 gctgccgggc gtcgggccct ggcgcacgta gatcttctgg ttggccggga agcacttgcg
      841 ggccgcgatc tcgcaggcgg tctgcgggca ctcgctgtgc tcgaagcagc cctcgcaggt
      901 gttcgtctcg aggttgcagt gcggcttgtc ggcctcgcac tggccctgca ccaccgcgtc
      961 gctggtgtca cactgcacgc agcgcccgtc gtcgaggcag atcgggcgcc cgccgtcggt
     1021 cgcgtcggtg cacgcgtcgg ccttgagcgc gccgcagccg acgcaggtgt cgacctcgca
     1081 gaacggccgg ttcgggtcct tggcgaggca ctcggcgctc tcgagcttgt cgccggtcgg
     1141 cgtgcactcg gggccgggcg gctcggtgtc gtcggtcgtg ctggtctggc cgccctcgct
     1201 gtcggtggtg gtgctggtgc ccgcggtcat cgtcgggtcg accgacgtat cgctgccagt
     1261 ggtcaggtcc tgggcgctct gacacacctt ctcggcggtg cagacgagcc cgggatcaca
     1321 ggcgccgccg ccggtacacg gacagccgag catgccgatc tcgcaggtga cgctggtcgt
     1381 ggtgtccggc gtccccgtcg tggtcatcgg cgcggtctcg gactgcgagt cctcgccggt
     1441 tccggggggc ccctcattcg gacacgcggc cagcacgagc ggggcgatga acgcgagacg
     1501 gctggaccag gcggggcaca tcaccgcgcc agtatccccg agcggcgacg agctgacaac
     1561 cgcccagcgg cccgcgcctg ctgccatcgg cgaccacgcg ccgccatcag ccgtgtccgc
     1621 cgtatccgcc gcgtccgccg cgtccgccgc tttgcagccg gcggttcatg tcagtgcaac
     1681 gagtgtgatg cgcatgacgc aaactacact cggagtaagg cgggacaagc aggtccgggg
     1741 tcggcccggg catttcttgc ggccctgggc ccaccgagcc actgaacagg cgaccagccg
     1801 tcagagcacg gggcggtccg cgcccgcgta gtccagagtc tcgttcacga tcgggcgaag
     1861 attctcgtcg aaatcgacgg cgggatcgcc tgtgagccag agggccacgt cctcgaggct
     1921 cccgccgatc ttggcctggt acacgccgtc ggcgtagccg gagaagacgt tcccgaggcc
     1981 ggcctt
//
LOCUS       DASWNJ010001879         2302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_33134, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001879 DASWNJ010000000
VERSION     DASWNJ010001879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33134"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VGB85_31315"
     CDS             <1..515
                     /locus_tag="VGB85_31315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138630.1"
                     /translation="TNTSGATTSGTTTDAPTTANPTTGTTTDGTTNPTSGTTGDAPTT
                     GVTTGEATTIGTMTSDGTTGGTTSDDTDGTTQGTLDTETTGGGLYGNCGWNVVGYYAC
                     VDKGGVAGTEDPLMKDEIACPQNIMEGAACNDMGMGGIGSVGCCTPEGVNFFCDVEDT
                     KKVFRDDCEI"
     gene            complement(574..2223)
                     /locus_tag="VGB85_31320"
     CDS             complement(574..2223)
                     /locus_tag="VGB85_31320"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY0138631.1"
                     /translation="MFSPPRGWLVAVVSGASLLACGEAAVVEMPVVRRPAAPVRPGKL
                     PEPEPELSSGGEAGDEGGAEASAEVGAETAAPLDLPSLLDPEPAAPPEDYGLIKPPVP
                     PPPPEALTVYAMAGYEVVTVYSQPDLASPKLGYMRIGTRMKVGERIDNEGCKNGFYAL
                     PAGGFACASKGLVVDRAKEVVSKFPPPAPRLDHALPYDYGFVRRWNGPMWWRVPTDLE
                     LQAMEVQRAERERVRLAAIVEKAPEKPAEPVTEPGEAAPVVDPAVPQEELLAMKLPLS
                     PDTPWLERGFFLSLGEKVVEQGRSYYRTARGAYVGASEVSRYEAKDFQGVALGEITYP
                     FGYTKKETKLIELTADNKLKAVRTLDKRAFVDLDEETEVGGKPYMITSERLLIRKDHL
                     NIPDPQPLPKGLEPWERWVDVSLSKQMLVAYEGTRPVYTTLVSTGKKGSPEDPYDTPK
                     GRWRIYSKHISTTMDGNSASDGNYSIQDVPWTMFFYGSYALHGAFWHRGFGAVRSHGC
                     VNLGPSDARWLFQWTTPFLPEGWHGVNSTDDSPGSTVIVHD"
BASE COUNT          363 a          857 c          747 g          335 t
ORIGIN      
        1 acaccaacac cagcggcgcg accacctccg gcaccacgac cgacgcgccg accaccgcga
       61 acccgaccac gggcaccacg accgacggga ccaccaaccc gacctccggc accacgggcg
      121 acgcgccgac gaccggcgtg acgaccggcg aggccaccac catcggcacg atgaccagcg
      181 atggcaccac tggcggcacc accagtgacg acactgatgg caccacccag ggcacgctcg
      241 acaccgagac caccggtggc ggtctctacg gcaactgcgg ctggaacgtc gtcggttact
      301 acgcgtgcgt cgacaagggt ggcgtcgccg gcaccgagga cccgttgatg aaggacgaga
      361 tcgcgtgccc ccagaacatc atggagggcg ccgcctgcaa cgacatgggc atgggcggga
      421 tcggctcggt cggctgctgc acccccgagg gcgtcaactt cttctgcgac gtcgaggaca
      481 ccaagaaggt cttccgcgac gactgcgaga tctaagacct cacctgcaca tacgcgaccc
      541 cggccctcac ccgagcgccg gggtcgtcgc gttttagtcg tggacgatga ccgtgctgcc
      601 cgggctgtcg tcggtcgagt tcacgccgtg ccagccctcg ggcaaaaacg gcgtggtcca
      661 ctggaacagc cagcgcgcgt cgctcggccc gaggttgacg cagccgtggc tgcgcaccgc
      721 cccgaagccc cggtgccaga acgcgccgtg cagcgcataa ctgccgtaga agaacatggt
      781 ccaggggaca tcctggatcg agtagttgcc gtccgacgcc gagttgccgt ccatcgtcgt
      841 cgagatgtgc ttgctgtaga tccgccagcg gcccttcggc gtgtcgtagg ggtcctccgg
      901 cgagcccttc ttcccggtcg agaccagcgt cgtgtacacc gggcgcgtgc cctcgtaggc
      961 gacgagcatc tgcttcgaca ggctgacatc gacccagcgc tcccacggct cgagcccctt
     1021 tggtagcggc tgcgggtcgg ggatgttgag gtggtccttg cggatcagca ggcgctcgga
     1081 ggtgatcatg tacggcttgc cgccgacctc cgtctcctcg tcgaggtcga cgaacgcccg
     1141 cttgtcgagc gtccgcaccg ccttgagctt gttgtccgcc gtcagctcga tcagcttggt
     1201 ctccttcttc gtgtaaccga aggggtacgt gatctcgccg agcgcgacgc cctggaagtc
     1261 cttggcctcg taacgcgaca cctcgctcgc accgacgtag gcgccgcgcg cggtccggta
     1321 gtaactccga ccctgctcga ccaccttctc gccgagcgac aggaagaacc cgcgctcgag
     1381 ccacggcgtg tcggggctca gcggcagctt catcgccagc agctcctctt gcggcaccgc
     1441 cgggtcgacc acgggggccg cctcgccggg ctcggtcacc ggctccgccg gcttctcggg
     1501 cgccttctcg acgatcgccg cgagtcgtac acgctcacgc tcggcccgct gcacctccat
     1561 cgcctgcagc tcgaggtcgg tcggcacgcg ccaccacatc ggcccgttcc agcggcgcac
     1621 gaagccgtag tcgtaaggaa gcgcgtggtc gaggcgcggc gccggcggcg ggaacttcga
     1681 gacgacctcc ttcgcgcggt cgaccaccag ccccttgctc gcgcacgcga agccgccggc
     1741 gggcagcgca taaaacccgt tcttgcagcc ctcgttgtcg atgcgctcgc cgaccttcat
     1801 gcgcgtaccg atccgcatgt agccgagctt gggtgacgcg aggtcgggct ggctgtacac
     1861 cgtcacgacc tcgtagccgg ccatcgcgta caccgtcagc gcctccggcg gcggcggcgg
     1921 cacgggcggc ttgatcaggc cgtagtcctc cggtggggcc gccggctccg ggtcgagcag
     1981 cgagggcagg tcgagcggcg ccgccgtctc ggcgccgacc tccgccgaag cttccgcccc
     2041 gccctcgtcg cccgcctcgc cgccgctcga cagctccggt tcgggctcgg gcaacttccc
     2101 cgggcgcacc ggcgcggccg gacggcgcac gaccggcatc tcgaccaccg ccgcctcgcc
     2161 gcaggccagc agcgacgctc cggacacgac ggccacgagc cagcctcggg ggggactgaa
     2221 catgcgcact gcatagcgca cgctccggcc gcggggaaga ttccggcaca ttcgcgagcc
     2281 gagcgcaaga aacgccctcc cg
//
LOCUS       DASWNJ010001880         4376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_344361, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001880 DASWNJ010000000
VERSION     DASWNJ010001880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344361"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VGB85_31325"
     CDS             <1..310
                     /locus_tag="VGB85_31325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY0138632.1"
                     /translation="IALVDEKMPVVVIALRGPGYGKVLSNLEEVRARGGKIIALATMG
                     DQQLGDLADDIILVPEIAEELQPILASIPLQLLAYHFADLKGTDVDQPRNLAKSVTVE
                     "
     gene            671..1735
                     /locus_tag="VGB85_31330"
     CDS             671..1735
                     /locus_tag="VGB85_31330"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0138633.1"
                     /translation="MFQVAGYTILHPLSEGGFGHVHLARRDVDGTLVAIKFLHSLSDD
                     NVTRFYREAKILHQHLGNPHVVRLFQSDFQGAVPYIVMEYCAGGSLRSWVRMKTDWLE
                     VAFALSNAAAGLSQIHEAGGFHRDIKPDNLLIAQSDDGTKIIKVGDFGLARVPKTAGH
                     PMTRSVWGTDGYMAPELFTGNEFTRACDVYSLGVTGLELLTGSRQPAKLQGRPDIPQA
                     FRELLLRMLRPLAAQRPTINVCQQILARLGQDEQAKREQALAQAELENRARENQVRAA
                     QQVKERQTQEAKARQAREAEAQQARSRQAAAAAAAAGTIGLGSIFLLAGGIALATMNA
                     RDQNGRFRGNDGRYRSGRWG"
     gene            complement(1831..2361)
                     /locus_tag="VGB85_31335"
     CDS             complement(1831..2361)
                     /locus_tag="VGB85_31335"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY0138634.1"
                     /translation="MLPTIRTPRLRLRAIGIGDVEEMFAVFSDPEVVRYWARPALTDL
                     AEAVAMIHQRAEQPSLLQWGIVRADEDRLLGTCTLADIDLAHRRASIGYALGRAAWGL
                     GIAREAVRALVKYGFEAIELHRIGADVDPRNTRSIRLLEELGFSREGLQRECYLVGDE
                     WQDTAMFGLLRREWAG"
     gene            complement(2365..3942)
                     /locus_tag="VGB85_31340"
     CDS             complement(2365..3942)
                     /locus_tag="VGB85_31340"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0138635.1"
                     /translation="MIEADHGGPADGQPRLEDAGPPRTVLPKRCPKCGERFPADFRVC
                     PRDAIELEAIVGGVNDPYLGSTLANSYRIDAIIADGGMGRVYEAHHTRLPGRALAVKI
                     LHRNLVGDTEVVSRFRREAEIADAVDHPNVARIFDVNNTADGVPFIVQELLVGEDLGQ
                     RLERDGRLSIADTVHILKQVCDVLAAVHARGVIHRDLKPNNLFLVGDPLRPTVKLIDF
                     GIARLKGPEDAKATQTGVVMGTPAFMAPEQARGERVDARADIYAVGAIAYCCATGRAP
                     YDLADAAAALHAVLMVEPPRPRAIAPDLPEDFEMVLQRAMARDREDRYPTLAALGEAF
                     AGFAGAEGAMVVPTSALQLGGGDLAMVARRARPELAAMSAVGLVLVVGGLAETLTAVF
                     TATSMPGAAGSLAVIASVAALATPAWLYARFLKQRVWPNSPQALAWSRNVGLTVSVGA
                     CAYAATYLLGRVFDLALGEGDGPDDWGRVAPWLIGMLAAVAAWYSRVRKTPTRARDLT
                     SPEVVSSAATMWVAPKG"
     gene            complement(4037..>4376)
                     /gene="moaC"
                     /locus_tag="VGB85_31345"
     CDS             complement(4037..>4376)
                     /gene="moaC"
                     /locus_tag="VGB85_31345"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEY0138636.1"
                     /translation="VGKGDVLGVARLAAISGAKRTPDLIPLCHPVRLTGVSVDFTLRE
                     DLPGVEVRVEVAAVDRTGPEMEAMTAAAAAVLTIYDMCKAMDRGMVIEAVELLEKSGG
                     KSGQWTRDGA"
BASE COUNT          741 a         1563 c         1395 g          677 t
ORIGIN      
        1 gatcgccctc gtcgacgaga agatgcccgt cgtcgtcatc gccctgcgcg gccccggcta
       61 cggcaaggtg ctgagcaacc tcgaggaggt ccgcgcgcgc ggtggaaaga ttatcgcgct
      121 cgcgaccatg ggcgaccagc agctcggcga cctcgccgac gacatcatcc tcgtgcccga
      181 gatcgccgag gagctccagc cgatcctcgc cagcatcccc ctgcagctgc tcgcgtacca
      241 cttcgccgac ctcaagggca ccgacgtcga ccagccgcgc aacctcgcga aatcggtcac
      301 cgtcgagtga tctgccgcag cgcaggcgca gggcgtacgc tcggcctgct ccacggacgc
      361 ccggaaaaac ctcacgcgtc tccaataaga cacgcgggcc ctgtgacgat cctttcatcg
      421 cttgccccgc gcccgtcagt agaccgagag gtaccccggc cgtccgtgga gcgtatcagc
      481 cggcccatga agcagtcgac gcgactccga ggacgtctag caagtcaagt ctccattatc
      541 tgcgtgatct cggacggatc atggccgcga gaggacacgc ctccgcattg actctcctcc
      601 gcggagattt atattgttag ttggtgagcg cgttcagaag ggaacgctgc tcatcccatc
      661 cggggtaaca atgtttcaag tcgctggcta tacaattctt catcctcttt ctgagggagg
      721 gttcggccat gtgcaccttg cacgaagaga tgttgacggg accctcgttg ccatcaagtt
      781 tctgcatagc cttagcgacg ataatgtcac gcgcttctac cgtgaagcga agatcctcca
      841 tcagcacctc ggcaaccctc atgttgttcg cctgttccag tcggacttcc agggggctgt
      901 tccatatatc gtgatggagt actgcgcggg agggtccctg cgaagctggg ttagaatgaa
      961 gaccgactgg ctcgaggttg ccttcgccct gtcgaatgcg gctgccgggc tgtcgcagat
     1021 ccatgaagcc ggcggctttc atcgggacat caagccagac aatctcctga tcgctcaaag
     1081 cgatgacgga accaagatca tcaaggtcgg cgactttggg ctcgcgcggg tgcctaaaac
     1141 ggcaggccac ccgatgacga ggagcgtatg gggcaccgac gggtacatgg ctcctgagtt
     1201 gttcacaggc aatgagttca cccgggcgtg tgacgtgtac tcactgggag tgactgggct
     1261 cgaattactg accggttcac gtcagccggc gaagttgcaa gggcggcctg acatcccgca
     1321 ggcgttccga gaacttctgc tccggatgct ccgtccactg gcggcccaac gcccgacgat
     1381 caatgtttgc cagcagatcc ttgcgcgcct cgggcaggac gagcaagcga aacgcgaaca
     1441 ggcgctcgcc caagcagaac ttgagaaccg agcgcgggaa aatcaggttc gcgcagccca
     1501 gcaagtcaag gagaggcaaa cccaggaggc caaggcacgg caggcccggg aagccgaagc
     1561 acaacaggct cggtccaggc aggctgctgc ggccgccgca gccgcaggaa caatcgggct
     1621 cggaagcatt ttcttgttgg cgggcggcat tgctttggcc acgatgaacg ctcgcgacca
     1681 aaatggcaga tttcggggaa acgacggcag atatcgtagt ggccgctggg gttgatcgag
     1741 tgcgccctcc gagggccgcg gccgctccgg cggcttatcc tcgttcgttg gctacaacac
     1801 gtgcctgtgc cgtcgagtga cggctgtatc tcagccggcc cactcgcgcc gcagcagccc
     1861 gaacatcgcc gtgtcctgcc actcgtcacc gacgaggtaa cactcgcgct gcaggccctc
     1921 gcgcgagaat ccgagctcct cgagcaggcg gatcgagcga gtattacgcg gatcgacgtc
     1981 cgcgccgatc cggtgcagct cgattgcctc gaagccgtac ttgaccaacg cccgcaccgc
     2041 ctcgcgcgcg attcccagcc cccacgccgc ccgcccgagc gcgtagccga tcgaagcccg
     2101 ccgatgcgcc aggtcgatgt cggcgagcgt acaggtgccg agcagtcgat cctcgtccgc
     2161 gcgcacgatg ccccactgca acagcgacgg ctgctcggcc cgctggtgga tcatcgccac
     2221 ggcctcggcg aggtcggtca gcgccggccg agcccagtag cgcacgacct cggggtcgga
     2281 gaacaccgcg aacatctcct cgacatctcc gatcccgatc gcacgcagcc gcagccgcgg
     2341 agtcctgatc gtcggcagca cggctcagcc cttcggcgcc acccacatcg tcgcggcgct
     2401 cgacacgacc tcgggcgagg tcaggtcgcg cgcacgcgtc ggcgtcttgc ggacccgcga
     2461 gtaccacgcg gccaccgcgg cgagcatccc gatcagccac ggtgccaccc tgccccagtc
     2521 gtcgggcccg tcgccctcgc cgagcgcgag gtcgaagact cgccccaaca gatacgtcgc
     2581 cgcgtacgcg cacgcgccga cgctgacggt caggccgacg ttacgcgacc acgccagcgc
     2641 ctgcgggctg ttcggccaca cgcgctgctt caagaatctc gcgtacagcc acgcgggtgt
     2701 cgccagcgcg gcgaccgacg cgatcacggc gagcgacccg gccgcgcccg gcatcgaggt
     2761 cgcggtgaac accgccgtca gcgtctccgc caaccccccg acaacaagca cgagcccgac
     2821 cgccgacatc gccgcgagct cgggtcgcgc ccgtctggcg accatcgcca ggtcgccacc
     2881 cccgagctgc aacgccgacg tcggcacgac catcgctccc tcggcccccg cgaagccggc
     2941 gaacgcctcg ccgagcgccg cgagcgtcgg gtagcggtcc tcgcggtcgc gggccatcgc
     3001 ccgctgcagc accatttcaa agtcctcggg caggtccggc gcgatcgccc gcggtcgcgg
     3061 tggttcgacc atcagcaccg cgtgcaacgc cgccgcagca tccgccaggt catacggcgc
     3121 cctgccagtc gcgcagcagt acgcgatcgc gccgaccgca tagatatcgg cgcgcgcgtc
     3181 gacccgctcg ccgcgcgcct gctcgggcgc catgaaggcc ggcgtgccca tcacgacgcc
     3241 cgtctgcgtc gccttcgcgt cctcgggccc cttgaggcgg gcgatgccga agtcgatcag
     3301 cttgacagtc gggcgcagcg ggtcgccgac caaaaataaa ttgttcggct tgaggtcgcg
     3361 gtggatcacc ccgcgggcgt gcaccgccgc gagcacgtcg cacacctgct tcaaaatgtg
     3421 caccgtgtcg gcgatgctga gccggccgtc gcgctcgagc cgttgcccga ggtcctcgcc
     3481 gaccagcagc tcttgaacga tgaacggcac cccgtcggcg gtgttgttga cgtcgaagat
     3541 gcgggccacg ttcgggtggt cgaccgcgtc ggcgatctcg gcctcacggc gaaaccgcga
     3601 cacgacctcg gtgtcgccca cgaggttgcg gtgcaagatc ttcaccgcca gcgcgcggcc
     3661 cggcagccgg gtgtggtgcg cctcgtagac gcggcccatg ccgccgtcgg cgatgatcgc
     3721 gtcgatgcgg tagctgttgg ccagcgtcga gccgaggtag gggtcgttga cccccccgac
     3781 gatcgcctcg agctcgatcg cgtcgcgcgg gcacacgcga aagtcggccg ggaaccgctc
     3841 cccgcacttc gggcagcgct tgggcagcac cgtgcgcggc ggaccggcgt cctcgagcct
     3901 cggctgcccg tcggcgggcc cgccgtgatc ggcctcgatc atggccggca gcataggcga
     3961 tcggcggccg cgcgcgccac ctgttccgcg ggtcccctac ccccgcagat ccccgcgcac
     4021 gcgcacgtct gcgcgctcag gcgccgtcgc gcgtccactg cccggacttg ccgccggact
     4081 tctcgagcag ctcgacggcc tcgatgacca tgccgcggtc catcgccttg cacatgtcgt
     4141 agatggtgag gacggcggcc gcggccgcgg tcatcgcctc catttcgggg ccggtgcggt
     4201 cgacggcggc gacctcgacc cggacctcga cgccgggcag atcttcccgc agcgtgaagt
     4261 cgacggagac cccggtgagg cggaccgggt ggcacagcgg gatgaggtcg ggcgtgcgct
     4321 tggcccccga gatcgccgcc agcctcgcca cccccagcac gtcccccttg ccgacc
//
LOCUS       DASWNJ010001881         3888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_455670, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001881 DASWNJ010000000
VERSION     DASWNJ010001881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455670"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VGB85_31350"
     CDS             <1..244
                     /locus_tag="VGB85_31350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138637.1"
                     /translation="KAAAKRLSQRVAEEAIAGLVRHGVARERLEPRGMGAEAPTARNR
                     TAEERAANRRVEFAPVDACRCGGPQDTRPVEQHLCR"
     gene            327..1322
                     /locus_tag="VGB85_31355"
     CDS             327..1322
                     /locus_tag="VGB85_31355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138638.1"
                     /translation="MKLMHSVGGAVAASVLACGGEGASTTDGSSSGTATTGNTSTTGG
                     TTGDELTTTSSTTAPTTADDTSTTDAPTTGETSTTSVTTDTSSTTDVSSSSSGGEELH
                     GCEGSMLAICEDFEDADDGAFPDGWGARGDEWGEGVIGVASDGAHDGVKALKIDGGNN
                     GQHFMDYKGDLGGLASHHYGRVFLKVAIPAPWPADGVLHADFIEGLGPGPGGSKHNVR
                     WGVVENTNMTMQWIYNVQPSNGDPEFAEGTPSQYTWSGEWQCMEWFYDEPSQHGTLWI
                     DGLEMPIVPGKNHAAEIPVFTSLGVGLANYQNAGEGFTVWFDDLAYDPERIGCEL"
     gene            complement(1368..3263)
                     /locus_tag="VGB85_31360"
     CDS             complement(1368..3263)
                     /locus_tag="VGB85_31360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138639.1"
                     /translation="MSAPRPWTPPALLLSALFITSLGAACGSPPATTGEPASTTGSES
                     GSTGPAEGSGTEPTTGVTGVMTTGTGSGTDPTTGGDPTTGDGPAPPDLQCPGDPSGQC
                     DDEPGAKLEAGASVQSIVPSCFETWVDLDNNFAYKKGNDTLNDCGCDRLCPGDPAYLA
                     PDEGEGDGKLQAVWMAGFGTGRPAAGVRGTGVGLVGDDDGLWARGTVLRQGNTTVAIV
                     ALDLVGWFNPDVQAVRAMLKEKGLEIDHLVVHSTHTHEGPDTMGLWGEELFISGYDPK
                     YRAQLRETVVAVIEAALLDARPVAALKVGEVDISTYHDNGVANVISDHRDPWVVDEFI
                     SAAQLLDGEGTSIATLVNFGCHPETLADDNLLMTSDFVHALRRTVELGSTWNTAPGQP
                     GLGGPVIYINGAVGGMMTTLGVEVTNPDGDTFKDASWEKADSIGQLLGEMALDAIANA
                     DEVTDPNLRVANTIFRVPVVNESFKFLFMQGTLEREVFINERTMGQEIETEMSLIELG
                     PIHMLTVPGELLPELAVGGYDGSRINAPGVPLIDPNNPNPPAVENAPKGPFIKDRMTS
                     TYRWIVGLGNDELGYIIPAYNFELGKPQPWVDEAEGDHYEETNSLGPDMAAIVDSYAD
                     LLMHAMK"
     gene            3329..>3888
                     /locus_tag="VGB85_31365"
     CDS             3329..>3888
                     /locus_tag="VGB85_31365"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEY0138640.1"
                     /translation="MPATRHYGFTDTGLVRAHNEDAFLADAGLGLFVVCDGVGGRARG
                     EIASQETVELILDWIKSDTTDIEIARTRALDEAGLQRLSALVRGAVQNACYMVHSMGE
                     IDPEQRGMSTTASLALVAGGVAIVGWVGDSRVYLCRAGEVSQITDDHTLVNHQVKQGL
                     LTAEQAKHSKMKHVITRAVGHRDFVEV"
BASE COUNT          595 a         1244 c         1447 g          602 t
ORIGIN      
        1 caaggcggcg gcgaagcggc tgagccagcg cgtggccgag gaggcgatcg cggggctcgt
       61 gcgccacggg gtcgcccgcg agcgcctcga gccgcggggg atgggcgccg aagcgccgac
      121 cgcccgcaac aggacggcgg aggagcgcgc ggccaaccgt cgcgtcgagt tcgcgccggt
      181 ggacgcctgc cgctgtggtg ggccgcagga cacgcgcccg gtcgagcaac acctgtgccg
      241 ctgagcggcg cgcggtcgcg agtgagggcg caagccctcg cgtaacgtcg tttgacgggt
      301 cgcggccggg ggtcgatgct gtgcgcatga agctcatgca ttcggtcggg ggagcggtgg
      361 ccgccagcgt gctggcctgt ggtggcgagg gagcaagcac gaccgacggg tcgtcgagcg
      421 ggaccgcgac cacggggaac acctcgacca ccgggggcac gaccggcgac gagctcacga
      481 cgacctcgag cacgacggcg ccgacgaccg cggacgacac gagcaccacg gacgcaccga
      541 ccaccggtga gacgagcacg acgagcgtca cgaccgacac cagtagcacg accgacgtct
      601 cgagcagctc gagcggcggc gaggagctgc acggctgcga gggctcgatg ctggcgatct
      661 gcgaggactt tgaagatgcc gacgacggcg ccttcccgga cggctggggg gcgcgcggcg
      721 acgagtgggg cgagggcgtg atcggtgtgg cgagcgacgg cgcgcatgac ggcgtcaagg
      781 cgctcaagat cgacggtggc aacaatggcc agcacttcat ggattacaag ggcgacctcg
      841 gcggcctcgc ctcgcatcat tacggccgtg tatttttgaa ggttgcaata ccggcgccgt
      901 ggccggcgga cggggtgttg cacgcggact tcatcgaggg tctcggcccg ggcccgggcg
      961 gcagcaagca caacgtccgc tggggcgtcg tcgagaacac caacatgacg atgcagtgga
     1021 tctacaacgt gcagccctcg aacggcgacc ccgagttcgc cgagggcacg ccgtcgcagt
     1081 acacctggag cggcgagtgg cagtgtatgg aatggttcta cgacgagccg tcgcagcacg
     1141 gcacgctgtg gatcgacggg ctcgagatgc cgatcgtgcc tggcaagaac cacgcggccg
     1201 agatcccggt gtttaccagc ctcggggtcg gcctcgccaa ctatcaaaat gccggcgagg
     1261 gttttactgt atggttcgac gacctggcgt acgacccgga gcggatcggc tgcgagctct
     1321 gacggccgct cagcggcaag acactcgttt cgcgcgcgac cgtcgcgtca cttcatcgcg
     1381 tgcatcagca ggtcggcgta gctgtcgacg atcgcggcca tgtcggggcc gagggagttg
     1441 gtctcctcgt agtggtcacc ctcggcctcg tcgacccacg gctggggctt gccgagctcg
     1501 aaattgtagg cggggatgat gtagccgagc tcgtcgttgc cgaggccgac gatccagcgg
     1561 taggtgctgg tcatgcggtc tttaataaat gggcctttcg gggcgttttc gacggcgggc
     1621 gggttggggt tgttcgggtc gatcagcggc acgccgggcg cgttgatgcg cgagccgtcg
     1681 tagccgccga ccgcgagctc gggcagcagc tcgccgggga ccgtcagcat atgaatcggc
     1741 ccgagctcga tcagcgacat ctcggtctcg atctcctggc ccatcgtgcg ctcattgata
     1801 aacacctcgc gctcgagcgt gccctgcatg aacaggaatt tgaaggattc gttgacgacc
     1861 gggacgcgga agatcgtgtt ggcgacgcgc aggttcgggt cggtgacctc gtcggcgttg
     1921 gcgatcgcgt cgagggccat ctcgccgagc agctggccga tcgagtcggc cttctcccag
     1981 ctggcgtcct tgaaggtgtc gccgtcgggg ttggtcacct cgacgccgag cgtcgtcatc
     2041 atgccgccga cggcaccgtt gatgtagatg accgggccgc cgaggccggg ctggccgggt
     2101 gcggtgttcc aggtcgatcc gagctcgacg gtgcggcgta gcgcgtgcac gaagtcggag
     2161 gtcatcagca ggttgtcgtc ggcgagggtc tccgggtgac agccgaagtt gaccagggtg
     2221 gcgatgctgg tgccctcgcc gtcgagcaac tgcgcggcgg agatgaactc gtcgacgacc
     2281 cacgggtcgc ggtggtcgct gatgacgttg gcgacgccgt tgtcgtggta ggtcgagatg
     2341 tcgacctcac cgaccttgag cgcggccacg ggccgcgcgt cgagcagcgc ggcctcgatg
     2401 acggcgacga cggtctcgcg cagctgcgcg cggtacttcg ggtcgtagcc cgatatgaac
     2461 aattcctcgc cccacaggcc catggtgtcg gggccctcgt gggtgtgggt cgagtggacg
     2521 acgaggtgat cgatctctag gcccttctcc ttcaacatgg cgcgcacggc ctgcacgtcg
     2581 gggttgaacc acccgacgag gtcgagcgcg acgatcgcca cggtcgtgtt gccctggcgc
     2641 agcacggtgc cgcgcgccca caggccgtcg tcgtcgccga cgagcccgac gccggtgcca
     2701 cgcacgcccg ccgcgggccg accggtgccg aagccggcca tccaaaccgc ctgcagcttg
     2761 ccgtcgccct cgccctcgtc gggcgcgagg taggccgggt caccggggca caggcggtcg
     2821 cagccgcagt cgttgagggt gtcgttgccc tttttgtagg cgaagttgtt gtcgaggtcg
     2881 acccaggtct cgaagcagct gggcacgatc gactgcaccg aggcgccggc ctcgagcttg
     2941 gccccgggct cgtcgtcgca ctggcccgag gggtcgcccg gacactgcaa gtccggcggc
     3001 gcggggccgt cgccggtcgt cgggtcgccg ccggtcgtcg ggtcggtgcc gctgccggtc
     3061 ccggtcgtca tgaccccggt cacgccggtg gtcggctccg tcccgctgcc ctcggccggc
     3121 ccggtactgc cagactcgct gccggtcgtg ctggcgggct cgcccgtggt ggccggcggc
     3181 gagccgcagg cggcccccag ggaggtgatg aagagagcag acaggagcag ggcagggggc
     3241 gtccaggggc ggggggcgga catttccgcg ccacgataac ccatgcgcca gcgccggcgc
     3301 cagggctccg tgttatgttc cgcgtccttt gccggcgacc cgtcactacg gttttaccga
     3361 caccggcctg gtccgggccc acaacgagga cgcgtttttg gcggacgcgg ggctcggcct
     3421 gttcgtcgtg tgcgacggcg tcggcgggcg ggcccgcggc gagatcgcca gccaggagac
     3481 ggtcgagctg atcctcgact ggatcaagtc ggacaccacc gatatcgaga tcgccaggac
     3541 gcgcgcgctc gacgaggcgg ggctgcaacg gctgtcggcg ctggtccgcg gggcggtgca
     3601 gaacgcctgc tacatggtcc acagcatggg agagatcgac cccgagcagc gcggcatgtc
     3661 gacgacggcc tcgctcgcgc tcgtcgccgg cggagtggcg atcgtcggct gggtcggcga
     3721 ttcgcgggtg tacctgtgcc gcgccggcga ggtctcgcag atcaccgacg accacacgct
     3781 ggtcaatcat caggtcaagc agggcctgct gaccgccgag caggccaaac actcgaagat
     3841 gaagcacgtg atcacccggg cggtcgggca ccgtgacttc gtcgaggt
//
LOCUS       DASWNJ010001882         1515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_344409, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001882 DASWNJ010000000
VERSION     DASWNJ010001882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344409"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VGB85_31370"
     CDS             complement(<1..900)
                     /locus_tag="VGB85_31370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0138641.1"
                     /translation="MHQALTADASLPLAAPSIADPEAVDAAATEPAEPAPAGSARDSR
                     DEVSSARDPLGPYFRDIASFDVMGREAELEAATRIVHLRRSFWRNALAYPPFIEGICD
                     LLPQLLTDLPAEPVAEMKRAARALRDRDLVVNQKAYEAAREQLTAVLTELDLAGNASD
                     RIVADLLSIESGANESLGLKVKLPPRGSGPFLAYLASVRAEHHALATAKSTFLTANLR
                     LVVAIARRFGTLRMPLQDLIQEGNLGLIKAIERFDHRRGFRFSTYASWWIRHAIGRAI
                     AEKGRAVRLPVHMLEAYNKVRKAR"
     gene            complement(1222..>1515)
                     /locus_tag="VGB85_31375"
     CDS             complement(1222..>1515)
                     /locus_tag="VGB85_31375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138642.1"
                     /translation="RHPKRYLGYLTDAGLDGKARYEETTHGKAALADLDWPVVVRGDG
                     REPAAARFARSMLADLADMLGPSPYLPEAEALEPADCHALTWPPPHHNVLRNL"
BASE COUNT          215 a          470 c          603 g          227 t
ORIGIN      
        1 ccgggccttg cggaccttgt tgtaggcctc gagcatgtgc accggcagcc gcacggcgcg
       61 gcccttctcg gcgatcgccc ggccgatcgc gtgccggatc caccagctcg cgtaggtcga
      121 gaagcgaaat ccgcgacggt gatcgaagcg ctcgatcgcc ttgatcagcc cgaggttgcc
      181 ctcctggatc aggtcctgca acggcatgcg cagggtgccg aagcggcgcg cgatggcgac
      241 gacgaggcgc aggttggcgg tcaggaacgt ggacttggcg gtggcgagcg cgtggtgctc
      301 ggcgcggacg ctggcgaggt aggcgaggaa ggggccgctg ccgcgcggcg gcagcttgac
      361 cttgaggccg aggctctcgt tggcgccgga ctcgatgctg agcaggtcgg cgacgatgcg
      421 gtcgctcgcg ttgccggcga ggtcgagctc ggtgagcacc gcggtgagct gctcgcgagc
      481 ggcctcgtag gccttttgat tgacgacgag gtcgcggtcc cgcagcgccc gcgcggcccg
      541 cttcatctcc gcgacgggct cggccggaag atcagtgagc agctgcggga gcaggtcaca
      601 gatgccctcg atgaagggcg gataggcgag ggcgttgcgc cagaagctgc gacgcaggtg
      661 gacgatgcgg gtcgcggcct cgagctcggc ctcgcgaccc atgacgtcga agctggcgat
      721 gtcacgaaag tacgggccca gtgggtcgcg cgccgaggag acctcgtcgc ggctgtcgcg
      781 ggccgacccg gcgggcgcgg gctccgcggg ctcggtggcg gccgcgtcga cggcttcggg
      841 atctgcgatc gagggcgcgg ccaggggcag cgaagcgtcg gctgtcaggg cttgatgcat
      901 gcgcggtggg ccttccgtgt cattcgaccg gcgccgcggt cgaggctcca gcgtccgcca
      961 gcgaagaaag gcctgtcaac ctgcgttcaa ggttcgccgt atgcgagtgg gttttcgcag
     1021 cctcgctcgc gcggcgcgtc gcccgcgaca tcaagctgcc gcgcacgcag ttcgaatgga
     1081 ggtttccagc gcgcacgcgc cgggcgaaag ctgacgaagc gcgaggaggg aatgtccagg
     1141 ccgccgcgag ggcggccgga gtgatctcgc cggtgggaca tgtgtcagcg ttcggcacgc
     1201 cgggcgactt cgtgggcccg gtcagaggtt tcgcaggacg ttgtgatggg gcggaggcca
     1261 ggtcagcgcg tgacagtcag ccggctcgag ggcctcggcc tcggggaggt atggggaggg
     1321 gccaagcatg tccgcgaggt cggcgagcat cgagcgcgcg aagcgagccg ccgcaggctc
     1381 gcgcccgtcg ccgcgaacga ctacgggcca gtcgaggtcc gccagcgcgg ccttgccatg
     1441 ggtcgtctcc tcgtagcgcg ccttgccgtc gagcccggcg tcggtgaggt agccgagata
     1501 gcgcttgggg tggcg
//
LOCUS       DASWNJ010001883         2699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_104753, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001883 DASWNJ010000000
VERSION     DASWNJ010001883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104753"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..948
                     /locus_tag="VGB85_31380"
     CDS             271..948
                     /locus_tag="VGB85_31380"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0138643.1"
                     /translation="MQTGADDGRQIKVSAFRWVPPFAQGVVRDMRVRWALEEAGVAYS
                     ARLLDVGENKSSDYRQLQPFGQVPVYEDGELVLFESGAIVLHIAERSPALMPEDAEGR
                     ARTRMWIFAALNTLEPSVQNLAELDLFNASETWAKLRRPGVVAAVRTRLADLAASLGD
                     SDYLEGRFTAGDLMMTTVLQILRHTELVEEFPALAAYRRRCEARPAFKKALADHMAPF
                     AANAPKA"
     gene            complement(1034..1462)
                     /locus_tag="VGB85_31385"
     CDS             complement(1034..1462)
                     /locus_tag="VGB85_31385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138644.1"
                     /translation="MLSTITLLASTLAFSPALSVAPVMATSGYNPGGIGISIGGGGGG
                     GDLDLDLDACLDLDLDIDLDCEIEVGAECHADCEVNVDAYCDAQANANKWNAKAHAQC
                     EAEVEAQCHANCDIDGSVFCEGDIYVGADICLDVSLDLDL"
     gene            2074..2520
                     /locus_tag="VGB85_31390"
     CDS             2074..2520
                     /locus_tag="VGB85_31390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR family protein"
                     /protein_id="HEY0138645.1"
                     /translation="MTGAIEFYGVGEPYGELSNFAPCPIFLQGKRWPTSEHYFQAQKF
                     ARTPHEEEVRRVKKPRIAAEMGRDRKRPLRRDWEAVKDDVMLAALRAKFTQHADLRAL
                     LLGTGDARLVEHTANDSYWADGGDGSGKNRLGELLMRVREELRAAG"
BASE COUNT          449 a          775 c         1037 g          438 t
ORIGIN      
        1 tctagaaata gatcgcataa cgtgcatccc ggcgcgccgg gctgggcggc tcgagaggcg
       61 taagtgttcg ggaacaaagg gattcttcgg cgatgggcgg cgcttcccgc ggcgttctgg
      121 cgggacgcgg gcggcgtgtc gatgaaatgc gacagcgaca cgatatgcac gtgtgcatgg
      181 acgtcaggtt tctcgggtcg cgcgcgcgag atgtgatgcc cgggtgcgcc cccgcttgaa
      241 ctcgcggacg tttcgcggga tagccgtgcg atgcagaccg gggctgacga cggcagacag
      301 atcaaggtga gcgcgtttcg ctgggtgccg ccgttcgcgc agggggtggt gcgagacatg
      361 cgggtccgct gggcgctcga ggaggcgggc gtcgcgtact cggcgcggct gctcgacgtc
      421 ggcgagaaca agtcgtcgga ctaccgccag ctgcagccct tcgggcaggt gccggtctac
      481 gaggacggcg agctggtgct gttcgagtcc ggggcgatcg tgctgcacat cgccgagcgc
      541 tcgccggcgc tgatgccgga ggacgccgag gggcgggccc ggacgcgcat gtggatcttc
      601 gccgcgctca atacgctcga gccatccgtg caaaacctcg ccgagctcga cctgttcaac
      661 gccagcgaga cgtgggcgaa gctgcggcgg cccggtgtgg tcgcggcggt gcggacgcgc
      721 ctggcggacc tggcggcctc gctcggcgac agcgattatc ttgaggggcg cttcaccgcc
      781 ggcgacctga tgatgacgac ggtgctgcag atcctccggc acacggagtt ggtggaggag
      841 ttcccggcgc tggcggcgta tcgccggcgg tgtgaggcgc ggcctgcctt taaaaaggcg
      901 ctcgcggatc acatggcgcc gttcgccgcg aacgcaccga aggcgtgaac ccgaagtttt
      961 cttaaaaagg ggggaacgcc gaaagggcgc tcacgcagtg cgtggcgccc gatcgggaga
     1021 ggatgaggag ggcttacagg tcgaggtcga ggctgacatc gaggcagatg tcggcgccga
     1081 cgtagatgtc gccctcgcag aagacagagc cgtcgatgtc gcagttggcg tggcactggg
     1141 cctcgacctc tgcctcgcac tgggcatggg ccttggcgtt ccacttgttg gcgttggcct
     1201 gggcgtcgca gtaggcgtcg acgttgacct cgcagtcggc gtggcactcg gcgccgacct
     1261 cgatctcgca gtcgaggtcg atgtcgaggt cgaggtcgag acaggcgtcg aggtcgaggt
     1321 cgaggtcgcc accaccacca ccgccgccga tgctgatgcc gatgccaccg gggttgtagc
     1381 cggaggtggc catgacgggg gcgacggaga gggcggggga gaaggcgagg gtggaggcga
     1441 gcagggtgat ggtggaaagc atggcagagg tccttttctt cgaggggtgt cgaaggagcg
     1501 cgtcgtgtct tgatggccgc cgggggattc cgtcgcggct cgatgagtcg attatgtcgg
     1561 cggaagatcg gcgggccacg acgacgagca agagaatgtg cgcacagtcg gcggcggcga
     1621 attctcgttt ggcgattgga cggtctagga ggccgtttcg gtgcgatctg cgaaattatc
     1681 ggcgcgctcg cggggggcgg cggcgcccgt gaaacgcggt actttgaccc catttcaaaa
     1741 cgcatcgtgt gtcgatcgtg cagcaccacg acccgagaaa aactcgcata acgagtctcc
     1801 gggtgcgccg gacggcactg gaggttttcg cgcaagtatt ggtgatgaaa ggggtttttg
     1861 gtgcgtcggg cggcgcgccc cgcggcgtct cgttcggagc ccggggcagt gtccataaaa
     1921 tgcaacaagg cggggatatg cacgtgtgca tgggcgtcag cttcctgggg ttcgccgcgg
     1981 gcacgatgcg atgtttgcca tgtctgggcg gacgcacgcg atgcggggcc gcccgggctc
     2041 cacgtccccg gtgcggcgcg gtaggatgcg cggatgaccg gcgcgatcga gttctacggg
     2101 gtcggggagc cgtacgggga gctctcgaac ttcgcgccgt gcccgatctt tctgcagggc
     2161 aagcgctggc cgaccagcga gcactacttc caggcgcaga agttcgccag gaccccgcac
     2221 gaggaggagg tccgcagggt caagaagccg aggatcgccg ccgagatggg acgggaccgc
     2281 aagcggccgc tgcgccgcga ctgggaggcg gtcaaggacg acgtcatgct cgcggccctg
     2341 cgcgccaagt tcacccagca cgcggacctg cgcgcgctgc tgctgggcac cggcgacgca
     2401 cgcctggtcg agcacaccgc caacgacagc tactgggccg acggcggcga tggcagcggc
     2461 aagaaccggc tcggcgagct gctgatgcgc gtgcgcgagg agctgcgcgc cgcgggttaa
     2521 aataaacgcc aaaaggcgcc cacgcggtgc gtggacgccc ttttggggag atgagggagg
     2581 ctcacaggtt gaggtcgagg ccgatgtcga ggcagatgtc ggcgccgacg tagatgtcgg
     2641 ccttgcagaa gacggagccg tcgatgtcgc agttggcgtg gcactgggcc tcgacctcg
//
LOCUS       DASWNJ010001884         1644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_416017, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001884 DASWNJ010000000
VERSION     DASWNJ010001884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416017"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VGB85_31395"
     CDS             <1..538
                     /locus_tag="VGB85_31395"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY0138646.1"
                     /translation="VDVRVIAATNRELRSEVNVGRFRADLYFRLAVITIGMPPLRQRP
                     EDIPALVDELLTALRAPPEQTEPLRAPDFVAQLCRAAWSGNVRELRNYLERCVVLRSA
                     FPLVGGEGEVASEEGFVVDPTAPYATERERALADFERRYFRGLLRLHDGKVSRAAGAA
                     GIDRAYLYRLLRRHGIDP"
     gene            535..>1644
                     /locus_tag="VGB85_31400"
     CDS             535..>1644
                     /locus_tag="VGB85_31400"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0138647.1"
                     /translation="MSDETPQRVTEVGEPARVTLPVSLAPDGRFAAAGEQLSLGPGSV
                     LKQYELIRELGRGGMGRVFLARDTRLARLCAIKLLLGYSGERAERFLSEARATARCKH
                     ESIVVIYEVDEHGGFPYMVLEYVEGRTLRAWLEERAGDEPLPARGVAEMMIPVVQALV
                     CAHEMGIVHRDLKPENIVLTEAGQIKVLDFGVAKQLVGDATRQALQPAERSLVGTPAY
                     MSPEQWLDLDVDAQTDLWAVGIMLHELATGQHPLAPAGPAQLAAVRELDVALPLRTLA
                     GPGAATLHGVVARCLCKRKAGRFASARALLVALEAVAGEQPASVGDDNPFAGLSAFQE
                     QDVAQFFGRERDTAAVVGRLRQHPLVVVAGPSGAGK"
BASE COUNT          202 a          570 c          642 g          230 t
ORIGIN      
        1 ggtcgacgtg cgggtgatcg cggcgaccaa ccgcgagctg cggtccgagg tcaacgtcgg
       61 caggttccgc gccgacctgt actttcgcct cgccgtgatc acgatcggca tgccgccgct
      121 gcgccagcga cccgaggaca tcccggcgct cgtcgacgag ctgttgaccg cgctgcgggc
      181 cccgccggag cagacggagc cgctgcgggc gccggacttc gtcgcgcagc tgtgccgcgc
      241 ggcgtggtcg ggcaacgtac gcgagctgcg aaattacctc gagcgctgcg tcgtgctgcg
      301 ctcggcgttc ccgctcgtcg gcggcgaggg ggaggtggcg agcgaggagg gcttcgtcgt
      361 cgacccgacg gcgccgtacg cgacggagcg cgagcgggcg ctcgccgact tcgagcgcag
      421 gtacttccgc gggctgctgc gcctgcacga cggcaaggtg tcgcgggccg cgggcgcggc
      481 ggggatcgac cgcgcgtatc tgtatcggct gctgcgtcgt cacggcatcg acccgtgagc
      541 gacgagacgc cgcagcgggt cacggaggtc ggcgagccgg cgcgcgtgac cctgccggtg
      601 tcgctggcgc ccgacgggcg cttcgcggcg gccggcgagc agctgtctct cgggccaggt
      661 agcgtgctca agcaatacga gctgatccgc gagctcgggc gcggcggcat gggccgcgtg
      721 ttcctggcgc gcgacacccg gctggcccgc ctgtgcgcga tcaagctgct gctcgggtac
      781 tcgggcgagc gcgcggagcg gttcctgtcc gaggcgcggg cgaccgctcg ctgcaagcac
      841 gagagcatcg tcgtcatcta cgaggtcgac gagcacggcg gcttcccgta catggtgctc
      901 gagtacgtcg agggccggac cctgcgcgcg tggctggagg agcgcgccgg cgacgagccg
      961 ctgccggccc gcggggtcgc cgagatgatg atccccgtcg tccaggcgct ggtctgcgcg
     1021 cacgagatgg ggatcgtgca ccgcgacctc aagccggaga acatcgtgct gaccgaggcc
     1081 gggcagatca aggtgctcga cttcggggtc gccaagcagc tcgtcggcga cgcgacccgg
     1141 caggcgctgc agccggccga gcgctcgctc gtcggcacgc cggcgtacat gtccccggag
     1201 cagtggctcg acctcgacgt cgacgcgcag acggacctgt gggcggtcgg catcatgttg
     1261 cacgagctcg ccaccggcca gcatccgctc gcgcccgccg gaccggcgca gctcgccgcg
     1321 gtgcgcgagc tcgacgtcgc gttgccgctg cggacgctcg cggggcccgg cgcggcgacg
     1381 ctgcacggcg tggtcgcgcg ctgtctttgc aagcgcaagg ccgggcgctt cgcctcggcg
     1441 cgcgcgctgc tcgtcgcgct cgaggcggtc gccggagaac agccggcgtc ggtgggcgac
     1501 gacaacccct tcgccgggct gtcggcgttt caggagcagg acgtcgccca gtttttcggc
     1561 cgcgagcgcg acacggcggc ggtggtcggt cggctgcgcc agcacccgct ggtcgtggtc
     1621 gcggggccgt cgggcgcggg caag
//
LOCUS       DASWNJ010001885         1896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_68540, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001885 DASWNJ010000000
VERSION     DASWNJ010001885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68540"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..939)
                     /locus_tag="VGB85_31405"
     CDS             complement(85..939)
                     /locus_tag="VGB85_31405"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0138648.1"
                     /translation="MSTFHDRVAVVTGAASGIGRALALALARRGARLALSDVDVAGLA
                     ETTRQARELGAEVFGERVDVAQRDAVGAHAEAVAAHFGAVHQVYNNAGVAFSRTVLDS
                     EYADYERVLAVNLFGVIHGTKAFLPRLIASGAGHVINISSLNGYMAQGQLSHYCASKF
                     AVRGFTEALRIEMLEAGHPVQVTCVHPGGIKTNIASNALARARAAGIPVTAADEARQR
                     TYNEKLLRMDAGLAATTILRGVERNSPRVLVGNDARAVDVLVRAFPALYQRAVVALGR
                     RLAASERR"
     gene            1021..>1896
                     /locus_tag="VGB85_31410"
     CDS             1021..>1896
                     /locus_tag="VGB85_31410"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0138649.1"
                     /translation="MSEHLDVLIVGAGLSGIGAAYHLQDRCPDKRYALLEARADLGGT
                     WDLFRYPGVRSDSDMHTLGYSFRPWNGAKAIADGPAILKYLRDTAQAYGIDQQIRYHH
                     RVIAASWSSAEARWTVEVERTDTGETLQLTCGFLLGCTGYYRYDHGHTPAFPGSERFR
                     GQIVHPQHWPEDLDYAGKRVVIIGSGATAVTLVPAMARTAAHVTMLQRSPSYITSLPS
                     VDPVAQQLHRVLPDRAVSSIVRWKNVLVQLGIFQLSRRRPELVKRLLRKMLARQLPAG
                     YDIDRHFTPRYDPWDQ"
BASE COUNT          275 a          721 c          625 g          275 t
ORIGIN      
        1 ccaggatcgc cagctcgcgg tcgtcgccgg tcgcccgccg cacccgtcgc gcgcgggtcg
       61 gtcgctcgtc tcgcgcgacc atcctcagcg ccgctcgctc gccgcgaggc gacgtcccag
      121 cgcgacgacg gcgcgttgat acagcgccgg gaaggcgcgc acgagcacgt cgaccgcgcg
      181 cgcgtcgttg ccgacgagca cccgcgggct gttgcgctcg acccctcgca ggatcgtcgt
      241 cgccgccagc ccggcgtcca tgcgcagcag cttctcgttg taggtgcgct ggcgggcctc
      301 gtccgcggcg gtgaccggga tcccggccgc ccgcgcccgc gccagcgcgt tgctcgcgat
      361 gttggtcttg atcccgcccg ggtgcacgca ggtgacctgc accgggtggc ccgcctcgag
      421 catctcgatc cgcagcgcct cggtgaagcc gcggaccgcg aacttcgagg cgcagtagtg
      481 actgagctgg ccctgggcca tgtagccgtt gaggctcgat atgttgatca cgtggcccgc
      541 cccggacgcg atcaatcgcg gcaagaacgc cttggtcccg tgaatgacgc cgaagaggtt
      601 gaccgcgagc acgcgctcgt aatccgcgta ctccgagtcg agcaccgtcc gcgagaacgc
      661 gacgccggcg ttgttgtaga cctgatgcac cgccccgaag tgggccgcga ccgcctccgc
      721 gtgggcgccg acggcgtcac gctgggcgac gtcgacgcgc tcgccgaaca cctcggcccc
      781 gagctcccgc gcctggcggg tcgtctccgc gaggccggcg acatcgacgt ccgacagcgc
      841 caggcgagcc ccacggcgcg ccagcgccag cgccagcgcg cgcccgatgc ccgatgccgc
      901 gcccgtgacc accgcgactc gatcgtgaaa ggtgctcatc ctgggccgac tctgggccgc
      961 ctcggcggat ctgtcaacac cgtgttgact aaatctacac catgtcgaat agtgccgggg
     1021 atgtccgaac acctcgacgt gctgatcgtc ggcgccggcc tgtccggcat cggcgcggcg
     1081 taccacctgc aggacaggtg ccccgacaag cgctacgcgc tgctcgaggc gcgcgccgac
     1141 ctcggcggca cctgggacct gtttcgttac ccgggggtcc gctccgactc ggacatgcac
     1201 acgctcggct actcgtttcg cccgtggaac ggcgccaagg cgatcgccga cggcccggcg
     1261 atcctgaagt acctgcggga caccgcgcag gcgtacggca tcgatcagca gatccgctat
     1321 caccaccgcg tgatcgcggc ctcgtggtcg agcgccgagg ctcgctggac cgtcgaggtc
     1381 gagcgcaccg acaccggcga gaccctgcaa ttgacctgcg gcttcctgct cggctgcacc
     1441 ggctactacc gctacgacca cggccacacc ccggcgtttc cgggcagcga gcgctttcgc
     1501 ggccagatcg tccatcccca gcactggccg gaggacctcg attacgccgg caagcgcgtc
     1561 gtcatcatcg gcagcggcgc gaccgcggtg acgctcgtgc cggcgatggc gaggaccgcc
     1621 gcccacgtga cgatgctgca gcgctcgccg agctacatca ccagcctgcc gagcgtcgac
     1681 ccggtcgcgc agcagctcca ccgcgtgctc cccgaccggg ccgtgtcgtc gatcgtgcgc
     1741 tggaagaacg tgctggtgca gctcggcatc tttcagctga gccggcgccg ccccgagctc
     1801 gtcaagcgcc tgctgcgaaa gatgctcgcg cgccagctcc ccgccggcta cgacatcgac
     1861 cgccacttca ccccgcgcta cgacccgtgg gaccag
//
LOCUS       DASWNJ010001886         1935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_629562, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001886 DASWNJ010000000
VERSION     DASWNJ010001886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629562"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1210
                     /locus_tag="VGB85_31415"
     CDS             113..1210
                     /locus_tag="VGB85_31415"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEY0138650.1"
                     /translation="MHPATAPRDDRSDTRLLAVDRRSGALRDAGITDLAALLRPGDLL
                     VVNDAATLPASLRARRVGPAGEGDELELRLTGGGDGSDPGGGSRVRAVLFGAGDWRSP
                     TERRPAPPSLAPGDVLALLGPDGPVPQDMSLVTATVEAVDPRARRLLTLRIDRSGAAL
                     WAALYRLGRPVQYSYLGAPLALWSVQTAFAARPWAAEMPSAGRPLSWSTLLDLRRRGV
                     EIVALTHAAGLSSTGDPALDALLPLPERYEIPAETVAAIARTRARGGRVIAAGTTVVR
                     ALEGCFADRGELYPGPGETALLLHAGFVPRVVDGLLTGMHDPSESHFHLLRAFAPEPV
                     LMAAWRHASAEGYLQHEFGDATLLAPDLALA"
     gene            complement(1365..1562)
                     /locus_tag="VGB85_31420"
     CDS             complement(1365..1562)
                     /locus_tag="VGB85_31420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138651.1"
                     /translation="MATKNKAPKEILVVGSKVKDVIRDAGLRSDGELIQAVSDKVHEL
                     LASAIERTKSNNRSTVRPHDL"
BASE COUNT          266 a          725 c          634 g          310 t
ORIGIN      
        1 ccagcctcgc cgacccggcc gaggtcgcgc gcaagatcgt cgccatgatc gacgacccca
       61 tccgcgccag caacggcgcc cgcctctccg cctccagctg ggaggtgcag tgatgcaccc
      121 cgcgaccgca ccccgcgacg accgcagcga cacccgcctg ctcgccgtcg accgccgcag
      181 cggagcgctg cgcgacgccg gcatcaccga cctcgccgcg ctgctgcgcc ccggcgacct
      241 gctcgtcgtc aacgacgccg cgaccctgcc cgcctcgctg cgcgcccgcc gggtcggccc
      301 cgccggtgag ggcgacgagc tcgagctgcg cctgaccggc ggcggcgatg gatccgaccc
      361 cggtggtgga tcgcgggtgc gcgccgtcct gttcggcgcc ggcgactggc gcagcccgac
      421 cgagcgccgc ccggccccgc cgtcgctcgc ccccggggac gtcctcgcgc tgctcggtcc
      481 cgatggacct gtcccgcagg acatgtccct ggtgacagcc accgtcgagg ccgtggaccc
      541 ccgcgcccgg cggctgctga ccctgcgcat cgatcgcagc ggggccgccc tgtgggccgc
      601 gctttatcgc ctcggccggc cggtgcagta cagctacctc ggcgcaccgc tggcgctgtg
      661 gtcggtgcag accgcgttcg cggcccggcc gtgggcggcc gagatgccgt cggccgggcg
      721 tccgctgtcg tggtcgaccc tgctcgacct gcgccgtcgt ggcgtcgaga tcgtcgcgct
      781 gacccacgcc gcgggcctgt cctcgaccgg cgaccccgcg ctcgacgccc tgctgccgct
      841 ccccgagcgt tatgagatcc ccgccgagac cgtcgccgcg atcgcccgca cgcgcgcccg
      901 gggaggtcgc gtgatcgcgg ccggcaccac cgtcgtgcgc gcgctcgagg gctgcttcgc
      961 cgatcgcggc gaactgtacc ccggccccgg ggagaccgcc ctgctgctgc acgccggctt
     1021 cgtcccgcgc gtcgtcgacg gcctgctgac cggcatgcac gacccgagcg agagccactt
     1081 tcacctgctc cgcgccttcg cgcccgagcc ggtcctgatg gccgcgtggc ggcacgcgag
     1141 cgccgagggt tacctgcagc acgagttcgg cgacgcgacc ctgctcgcgc cggacctcgc
     1201 cctcgcctga cctttcagac aggtcgtaac cacggcacaa aaaaatgaaa caaaaaaatg
     1261 aagagagccg tttccccgag gggagccggc tctcttcact ttgtaaaaag cacccggaaa
     1321 tcgcgtcagc tgaccacagg agtgagcagc gacgcgagct cgtctcacag gtcgtgcggg
     1381 cggacggtgc tgcggttgtt gctcttggtg cgctcgatgg cggaggcgag cagctcgtgg
     1441 accttgtcgg acacggcctg gatgagctca ccgtcgctgc gcagaccggc gtcacggatc
     1501 acgtccttga ccttgctgcc gacgacgagg atttccttgg gtgccttgtt cttggtggcc
     1561 attgtgtatt ctttcgctgt ggggtcgcta cgcgaccgtt tggagaggtc tgagtggatc
     1621 ggagaaaccg accccgttgc tcgccacgga ccataaaccc gaaaattcgc tttgcaaagg
     1681 caaaattgca gcgtgtgcgg cacttttttt cggggaggcc tcgcaagccc ggtgtagctg
     1741 cccgtctgcg agtgatcttt cgggaccttc gcgaccccct ctcgcgctgt cgtgccaact
     1801 cgagatccgc gttattcgaa cgaattccgg cgatctggcg cggtggcccc gtcaaaactc
     1861 gcgtacacgg gcctccgcgg ccgagatcat gcgctctcca cagcctcgcc gatctcccgg
     1921 aggcgatcga cgatc
//
LOCUS       DASWNJ010001887         5566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_245119, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001887 DASWNJ010000000
VERSION     DASWNJ010001887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245119"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1540
                     /locus_tag="VGB85_31425"
     CDS             <1..1540
                     /locus_tag="VGB85_31425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138652.1"
                     /translation="FRVLPLALAVAAGSVTGCGESRKDKLEREMAYANAGKKTDDAGN
                     PIPEIPPDPTREALRPLLAEIYSNKERLPDVVEADIDVKDGLNYSLTPGVMAVIRVKK
                     GLPKDEKIKAIVQGTAEADSWTFRKNARSDYGGIIEKIKYSYGDPTKDKILRAYADLK
                     LIAFFNSPEAAKAIGELPAEVKGTVEAMKKDYSEGRDAHWNKWMEVKMYARRKVGGDE
                     PFRSVLRQISRDLGQEELPPRTFADSVDPQFKGWVSEIQNDEKLLILLTNMKELKDRV
                     EFLGETHTIWAVQGSQLIPEKARGVAIDKETGYGFFREDLGGGYNDLTFVFSPKLSGD
                     SLKRAFIHSMLFRQLLTDYQMLATAGGDFAKKGPDGGVDPNTSVVPDKYDPLYARCGA
                     TAALDTMLVGYAGTFKLLAGMSSKMKDSSKALTAAHKCIIEGAKPDIKIPAKDDEFDT
                     EGPAPASRLAIYQMLARFEKFDVDTAALAGDKRMAEDDTIEEQEKLLKQLKAQAEANK
                     KNEE"
     gene            complement(1586..2425)
                     /locus_tag="VGB85_31430"
     CDS             complement(1586..2425)
                     /locus_tag="VGB85_31430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138653.1"
                     /translation="MLLRDALGVVVPPYRTLAVSGAALDQMASIEFRADLVVEFRDDT
                     GERPVMSAVVEVQLRIDEAKQFSWPVYATVERSRKRCETCLIVITPDAAVAAWARRPI
                     RVGPGMELRPLVLGPAEIPTITDLEAGLANPILAVLSALTHGNEAEQGLPVVLVALEA
                     LGALDSGDARVYFNTIFKTLAEPMGRALRKEFMLRDQVPDFELSPDAQAIWDILQRRR
                     ALKAQIEAHAAAKMLLRILGRRGIALSPEDVERISTCEKMEQLELWADRALNVETAAE
                     LFA"
     gene            2596..4650
                     /gene="ligA"
                     /locus_tag="VGB85_31435"
     CDS             2596..4650
                     /gene="ligA"
                     /locus_tag="VGB85_31435"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEY0138654.1"
                     /translation="MTQRTQSRSEADLLALDAAAVQAGLPKFDADELEALIRRGNVEY
                     WDKHAPTLPDTLYDQLVERLRRVRPDAAVLDSMGPSQTGEPALAADEAVLLPPEERFG
                     AGVIHKRPMLSLDKCYNEADLHSWAAKFTGEIVVMPKMDGVACSLRYDKAGKLVLAAT
                     RGSGVEGEDITTNALAIKSIPSALKTGGAELEIRGELYMKLSEFQKFKEDFANPRNLT
                     AGSIKQKDRSKSRAAHLSFFPYDVIGPDFVDEREKFALFKKLGFAEIDHEFVPREQLQ
                     AAYERFAKNRASLDYEIDGVVYRASSIKEQNRLGLTGHHPRYAIAYKFQGDSGVTDLK
                     GVLWSVSRTGVITPVALLEPVELSGAMIGRASLHNLSIFEALGVTHDCRVEVTRRGGV
                     IPNVERVIEAGKHARPYEVPTHCPACNSPTTRQKKREGEFLFCSQPKQCVSARLGELE
                     HFAKAVDIQGFGPKVITAAVDAGLLQSPADFYKLRAEDLEELERLGRKSAQNLIAEIE
                     AKRELPLQVFLRALGAPHLGDQNAKLLAGEYETLEKIRKLTRDDLMEIKGIKEAIADA
                     LVEGLASRSELIDALLKHVKVQRGEKAAPVAGGALAGKSFVFTGTLEGFTREQAQERV
                     VALGGEVPSGVNKALSYLVVGAGKGAKSSKQKKAEELIAAGAALKVLSEDEFLELTGG
                     IE"
     gene            complement(4654..5298)
                     /locus_tag="VGB85_31440"
     CDS             complement(4654..5298)
                     /locus_tag="VGB85_31440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138655.1"
                     /translation="MRRLFILALGTCLIGHVVQDSLAAPAPAAAPEEPLARAASNRVK
                     LWQKAGTGANLTARYRLTRRSSLLFEPLGTAGSLRFTAPATLELRDDEPTGATTRVTA
                     GALEIVANDPTLPAGPTLGDGSPVRRWLRDRLLALLGARDLVALRAEARLRPVFGLRT
                     AFELSPAPGHPARAAIHDLAVQLDPESGELVRLVLTETGGDVVTLTLSDHQRTP"
     gene            complement(5295..>5566)
                     /locus_tag="VGB85_31445"
     CDS             complement(5295..>5566)
                     /locus_tag="VGB85_31445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138656.1"
                     /translation="LLLPPLLLVGALAADAAARSYAPAAGRPVVILACQLSAAPVLLV
                     SADPHWRSFGLLLGLGGVLGFALATRAAPALYARLRKLLREEVTP"
BASE COUNT         1005 a         1867 c         1945 g          749 t
ORIGIN      
        1 ctttcgcgtc ctacccctcg cgctcgcggt tgctgcgggc agtgtcactg gctgtggcga
       61 gagccgcaaa gacaagctcg agcgcgagat ggcctacgcg aacgccggca agaagacgga
      121 cgacgcgggc aacccgatcc ccgagatccc gccggacccg actcgcgagg cgctgcggcc
      181 gctgctcgcg gagatctaca gcaacaagga gcgcctcccc gacgtggtcg aggccgacat
      241 cgacgtcaag gacggcctga actactcgct gacgccgggc gtgatggccg tcatccgcgt
      301 caagaagggc ctgcccaagg acgagaagat caaggcgatc gtgcaaggca ccgccgaggc
      361 cgactcgtgg accttccgca agaacgcgcg cagcgactac ggcggcatca tcgagaagat
      421 caagtacagc tacggcgacc cgaccaagga caagatcctc cgcgcctacg ccgacctcaa
      481 gctgatcgcg ttcttcaact cgcccgaggc cgccaaggcg atcggcgagc tgccggccga
      541 ggtcaagggc accgtcgagg cgatgaagaa ggactacagc gagggtcgcg acgcccactg
      601 gaacaagtgg atggaggtca agatgtacgc ccgccgcaag gtcggcggcg acgagccctt
      661 ccgcagcgtg ctgcgccaga tctcgcgcga cctcggccag gaggagctgc ccccgcgcac
      721 cttcgccgac tcggtcgacc cgcagttcaa gggctgggtc tccgagatcc agaacgacga
      781 gaagctcctc atcttgctga ccaacatgaa ggagctcaag gaccgcgtcg agttcctcgg
      841 cgagacccac acgatctggg ccgtgcaagg ctcgcagctg atccccgaga aggcccgcgg
      901 cgtcgcgatc gacaaggaga ccggctacgg cttcttccgc gaggacctcg gcggcggcta
      961 caacgacctc accttcgtgt tctcaccgaa gctctcgggt gactcgctca agcgcgcgtt
     1021 catccactcg atgctgtttc gccagctgct gaccgactac cagatgctcg ccaccgccgg
     1081 cggtgacttc gcaaagaagg gccccgacgg cggcgtcgac ccgaacacct cggtggtgcc
     1141 cgacaagtac gacccgctgt acgcccgctg cggcgcgacc gcggcgctcg acacgatgct
     1201 cgtcggctac gccggcacct tcaagctgct cgccggcatg agctcgaaga tgaaggactc
     1261 gagcaaggcc ctgacggcgg cccacaagtg catcatcgag ggcgccaagc cggacatcaa
     1321 gatccccgcc aaggacgacg agttcgacac cgaaggcccg gcccccgcca gccgcctcgc
     1381 catttatcag atgctcgcgc gcttcgagaa gttcgacgtc gacaccgccg ccctcgccgg
     1441 cgacaagcgc atggccgagg acgacacgat cgaggagcaa gagaagctcc tcaagcagct
     1501 caaggcccag gccgaggcca acaagaagaa cgaggagtag aggcgcgggc tcgcaaagag
     1561 ccccagcgtc acaacaagac ggtcgtcagg cgaacagctc ggcggccgtc tcgacgttga
     1621 gggcccggtc ggcccagagc tcgagctgct ccatcttctc gcaagtggag atgcgctcga
     1681 catcctccgg tgagagcgcg atgccccgac gaccgaggat ccgcagcagc attttggccg
     1741 cggcgtgcgc ctcgatttgc gccttgagcg ccctgcggcg ctgcaagatg tcccagatcg
     1801 cctgcgcatc ggggctgagc tcgaagtcgg ggacttgatc gcggagcatg aattccttcc
     1861 tcagagcgcg acccatgggc tcggccaggg tcttgaagat agtattaaag tagacccggg
     1921 cgtccccgct gtccaacgcc cccagcgcct cgagcgcgac aaggacgacc gggaggccct
     1981 gttctgcctc gttgccgtgg gtcagcgccg aaagaacggc gaggatcggg ttggcgaggc
     2041 cggcctcgag atcagtgatg gtcgggatct ccgccgggcc gagcacgagc gggcgcagct
     2101 ccatgcccgg gccgacgcgg atcggtcggc gcgcccacgc cgcgacggca gcgtcgggcg
     2161 tgatgacgat caggcaggtc tcgcagcgct tgcgtgagcg ctcgaccgtc gcgtagacgg
     2221 gccagctaaa ctgtttcgcc tcgtcgatcc gcaactgcac ctccacgacg gcgctcatca
     2281 ccgggcgctc gccggtgtcg tcgcggaact cgacgacgag gtcggcacga aactcgatcg
     2341 acgccatttg atcgagcgcc gcccccgaca ccgccagcgt tcgatagggc ggcacgacca
     2401 caccgagcgc gtctcgtaac agcatcggaa tcagctctgg acgcgtacga aagatctcga
     2461 ccagcgcggt gtgctcgacc gatggcatca cgggctggtt aacacgtgct gcgaggccgt
     2521 ccaacgtttc gagcgttttc gccccgcggc acgaagcgca ccatggacaa catccggggc
     2581 tatcatgccg gccgcatgac gcagaggacg cagagccgca gtgaagccga ccttttggcc
     2641 ctcgacgccg ccgcggtgca ggcggggtta cccaagttcg acgccgacga gctcgaggcg
     2701 ttgatccgcc gcggcaacgt cgagtactgg gacaagcacg cgccgacgct gcccgacacg
     2761 ctttatgatc agctcgtcga gcggctgcgt cgcgtgcggc cggacgcggc ggtgctcgac
     2821 tcgatggggc cgtcgcagac cggcgagccg gcgctggcgg ccgacgaggc ggtgttgttg
     2881 ccgccggagg agcgcttcgg ggccggcgtg attcacaagc ggccgatgct gtcgctcgac
     2941 aagtgttaca acgaggccga cctgcacagc tgggcggcga agttcaccgg cgagatcgtc
     3001 gtgatgccga agatggacgg cgtcgcgtgc agcctgcgct acgacaaggc cggcaagctg
     3061 gtgctcgcgg cgacgcgcgg ctcgggggtc gagggtgagg acatcacgac caacgcgctg
     3121 gcgatcaagt cgatcccgag cgcgctcaag accggcggcg ccgagctcga gatccgcggc
     3181 gagctgtaca tgaagctcag cgagttccag aagtttaaag aggacttcgc caacccgcgc
     3241 aacctgacgg ccggttctat caagcaaaag gaccgcagca agagccgcgc ggcccacctg
     3301 agcttcttcc cgtacgacgt catcggcccc gacttcgtcg acgagcgcga gaagttcgcc
     3361 ctcttcaaga agctcggctt cgccgagatc gaccacgagt tcgtgccgcg cgagcagctg
     3421 caggccgcct acgagcggtt cgccaagaac cgcgcgagcc tcgattacga gatcgacggc
     3481 gtcgtctacc gcgcctcgtc gatcaaggag cagaaccggc tcgggctgac ggggcatcac
     3541 ccgcgctacg cgatcgccta caagttccag ggcgacagcg gcgtcaccga cctcaagggt
     3601 gtgctgtggt cggtcagtcg cacgggcgtg atcacgccgg tcgcgctgct cgagccggtc
     3661 gagctcagcg gcgcgatgat cggccgggcc agcctgcaca acctgagcat cttcgaggcg
     3721 ctcggggtga cccacgactg ccgcgtcgag gtcacccgtc gcgggggcgt gatccccaac
     3781 gtcgagcgcg tgatcgaggc cggcaagcac gcccgcccct acgaggtccc cacgcactgc
     3841 cccgcgtgca acagcccgac gacgcgccag aagaagcgcg agggcgagtt tttgttctgc
     3901 agccagccca agcagtgcgt ctcggcgcgg ctcggcgagc tcgagcattt tgcgaaggcg
     3961 gtcgacattc agggcttcgg gcccaaggtg atcaccgcgg cggtcgacgc cgggctgctg
     4021 cagagccccg cggattttta caagcttcgt gcggaggacc tcgaggagct cgagcgcctc
     4081 ggccgcaaga gcgcgcagaa cctgatcgcg gagatcgagg ccaagcgcga gctgccgctg
     4141 caagtattcc tgcgggcgct cggggccccg cacctcggcg accagaacgc caagctgctc
     4201 gccggcgagt acgagactct tgaaaagatc cgcaagctga cgcgcgacga cttgatggag
     4261 atcaaaggca tcaaggaggc gatcgccgac gcgctggtgg agggcctcgc ctcgcgcagc
     4321 gagctgatcg acgccctttt gaagcacgtc aaggtccagc gcggcgagaa ggcggcgccg
     4381 gtcgccggtg gggcgctggc gggcaagagc ttcgtgttca ccggcacgct cgagggcttc
     4441 acgcgcgagc aggcgcagga gcgggtggtc gcgctcggcg gcgaggtgcc gagcggcgtc
     4501 aacaaggcgc tgagctatct ggtggtcggc gcgggcaagg gcgccaagtc gagcaagcag
     4561 aagaaggccg aggagctgat cgccgccggt gctgcgctca aggtgttgag cgaggacgag
     4621 ttcctcgagc tcaccggcgg catcgagtga gcctcagggc gtgcgctggt ggtccgagag
     4681 ggtgagcgtc acgacgtcgc cgcccgtctc ggtcagcacc agccggacca gctcgcccga
     4741 ctccgggtcg agctggaccg cgaggtcgtg gatcgcagcg cgggccgggt gaccgggggc
     4801 gggcgagagc tcgaaggcgg tgcgcaggcc gaacaccggg cgcaggcggg cctccgcgcg
     4861 cagggccacg aggtcgcgcg cgccgagcag cgcgagcagc cggtcgcgca gccagcgacg
     4921 caccggcgag ccgtcgccga gcgtcgggcc tgccggcaag gtcgggtcgt tggcgacgat
     4981 ctcgagcgcg ccagcggtga cgcgggtggt cgcgccggtc ggctcgtcgt cgcgcagctc
     5041 gagcgtcgcc ggggccgtga agcgcaggct gccggcggtg ccgagcggct cgaacagcag
     5101 cgaggaccgg cgggtcaggc ggtagcgggc cgtcaggttg gcgccggtgc cggccttctg
     5161 ccacagcttc acgcggttgc tcgccgcgcg cgcgagcggc tcctcggggg cggcggccgg
     5221 ggccggggcg gcgaggctgt cctggacgac atgcccgatc aggcatgtcc cgagggccag
     5281 gatgaagagg cgcctcacgg tgtgacctcc tcgcgcaata actttcgcag ccgggcgtac
     5341 aacgccggcg cggcgcgggt cgccagcgcg aagccgagca cgccgcccag gccgagcagc
     5401 aggccgaagc tgcgccagtg cgggtcggcg ctgaccagca acaccggcgc cgccgagagc
     5461 tgacaggcga ggatcaccac cggtcggccg gctgcgggtg catagctgcg cgccgcggcg
     5521 tcggcggcga gcgccccgac gagcagcagc ggcggcagca gcagcg
//
LOCUS       DASWNJ010001888         1717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_344508, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001888 DASWNJ010000000
VERSION     DASWNJ010001888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344508"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /gene="rho"
                     /locus_tag="VGB85_31450"
     CDS             <1..706
                     /gene="rho"
                     /locus_tag="VGB85_31450"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEY0138657.1"
                     /translation="TVMMQHMAHAIAANYPDSYMMVLLVDERPEEVTEMQRSVKAEVI
                     ASTFDEPAARHVHVAEMVIERAKRLVELKKDVVILLDSITRLARAYNNVVPSSGKVLT
                     GGVDANALQRPKRFLGAARNVEEGGSLTIIGTALIDTGSKMDEVIFEEFKGTGNSEIV
                     LDRRVADKRVFPAIEINKSGTRKEELLVDRATLSRMWVLRRILNPMGTIDAVEFLLDK
                     MKHAKTNEDFWGSMNS"
     gene            866..>1717
                     /locus_tag="VGB85_31455"
     CDS             866..>1717
                     /locus_tag="VGB85_31455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0138658.1"
                     /translation="MLAVLVVLMLGAGAAGAYWKFVMEPERQAAAGGPVPGGGGRGGG
                     ARGPTPVEAAEVRIGPVEVTTEAVGSLLSNEAVVIRPEVTGRVAAINFDEGAPVKAGQ
                     VIVELDAAVERAQLAQAEADLGLGTANFERAKELRRSNVGTQRALDEAQAQLRTAEAA
                     IQLAKARLSKLTLTAPFDAVAGLRRVSVGDFVTAGTDIVGLEQIKPLKVDFSIPEIFL
                     RSVREGQRIAFTVDAFPGEAFEGVVFAVSPLVSEAGRAVTLRARIDNTDVRLRPGLFA
                     RVTLTLSQ"
BASE COUNT          268 a          597 c          608 g          244 t
ORIGIN      
        1 gacggtgatg atgcagcaca tggcgcacgc catcgcggcc aactatccgg acagctacat
       61 gatggtgctt ctcgtcgacg agcggccgga agaagtgacc gagatgcagc gttcggtgaa
      121 ggccgaagtc atcgcctcca ccttcgacga gcccgccgcc cgccacgtgc atgtggccga
      181 aatggtgatc gagcgcgcca agcgcctggt ggagctgaaa aaagacgtgg tgatcctgct
      241 ggactcgatc acccgcctgg cccgcgccta caacaacgtc gtgccctcgt cgggcaaggt
      301 gctcaccggc ggcgtcgacg ccaacgccct gcagcgcccg aagcgcttcc tcggcgccgc
      361 ccgcaacgtc gaggaaggcg gctcgctgac catcatcggc accgcgctga tcgacaccgg
      421 ctcaaagatg gacgaggtca tcttcgagga attcaaggga accggcaatt ccgagatcgt
      481 gctcgaccgc cgcgtcgcgg acaagcgcgt gttcccggcg atcgagatca acaagtccgg
      541 cacccgcaag gaggagctgc tggtcgaccg cgccacgctc tcgcggatgt gggtcctgcg
      601 ccgcatcctg aacccgatgg gcacgatcga cgcggtcgag ttcctgctcg acaagatgaa
      661 gcacgcgaag acgaacgagg acttctgggg ctcgatgaac tcctagaggc agccggcccg
      721 gcaggccgtc gacggctggc cggggcgctc gcgcgggcgt tacatcccgt tacacatccg
      781 tcgcttccag acgcgcggcg gcaccctatc tctggtcctg gtccgccctc aggcgtcgcc
      841 ccggagaagc cgtgtgctcc gcaccttgtt ggccgtccta gtcgtcctga tgctcggcgc
      901 cggtgcggcc ggtgcgtact ggaagttcgt catggagccg gagcggcagg ccgccgcggg
      961 cgggccggtt cccggtggcg gcggccgagg cggcggggcg cgcggcccga ccccggtcga
     1021 ggcggcggag gtccggatcg gccccgtcga ggtcacgacc gaggccgtgg gctcgctgct
     1081 ctcgaacgag gcggtcgtga tccggcccga ggtgacgggc cgggtggccg ccatcaactt
     1141 cgacgagggc gcgccggtca aggccgggca ggtcatcgtc gagctcgacg ccgcggtgga
     1201 gcgcgcccag ctcgcccagg ccgaggccga cctcggcctc gggacggcga acttcgagcg
     1261 cgcgaaggag ctgcgccgca gcaacgtcgg gacgcagcgc gcgctcgacg aggcgcaggc
     1321 ccagctgcgc acggccgagg ccgcgatcca actggcgaag gcccggctct cgaagctgac
     1381 gctcacggca cccttcgacg cggtggccgg cctgcgccgg gtcagcgtcg gcgacttcgt
     1441 gaccgccggc accgacatcg tcgggctcga gcagatcaag ccgctcaagg tcgacttctc
     1501 catcccggag atcttcctgc gctccgtccg cgaggggcag cggatcgcgt tcacggtcga
     1561 cgcgttcccg ggcgaggcgt tcgagggcgt ggtcttcgcg gtgagcccgc tggtgagcga
     1621 ggccggccgg gcggtcacgc tgcgcgcccg gatcgacaac accgacgtcc gcctgcggcc
     1681 cggcctgttc gcgcgggtga cgctcaccct ctcgcag
//
LOCUS       DASWNJ010001889         6043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_317349, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001889 DASWNJ010000000
VERSION     DASWNJ010001889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6043
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317349"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VGB85_31460"
     CDS             <1..864
                     /locus_tag="VGB85_31460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138659.1"
                     /translation="PRVVPGLVLESSRGAPADVGPRLEPIGDGRLRHRGKGFSAIVDA
                     DGSVEFCDVIVEGKTSVLGFDLRGRKNPPANLARDNFEERALYPGGPATAPVMVGVGG
                     GFGGIVGALISKVSQRRGPGRSPGRRYTAAKLQFLRDTEAMRMRMTHAWLKAQLATQL
                     DLLIAQVLAVWRDTSVPLVERRRRIFVMWDECEEPGPVSESAVERIRGDAAALARTRI
                     EALVRLLAPAGSPRAYPRDELARLNTTRRSRRAFDPYGSSGAAGVGAIDEVEPLEQPP
                     RDDDAVPAAPP"
     gene            complement(772..1965)
                     /locus_tag="VGB85_31465"
     CDS             complement(772..1965)
                     /locus_tag="VGB85_31465"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0138660.1"
                     /translation="MQPWRHSPRDAAVVAPPLALALLAPILPWLAGHGLGGMSLATAA
                     IALTVWWLSNTVAHIHLHTPVFRRRTYNRLFSLLLSLLTGVPQAIWRHRHLAHHRDQA
                     PGPLRPGRYGSLEIAAVAQLWLALAVTLPAFFLATYLPGFLLGLGLCQLQGRGEHLAG
                     PGGISHDAALHNRLWFNDGYHAEHHRWPGAHWTTLPARRLADAPRSRFPPVLRNFLVT
                     RPSIPCLLGALERLALACPPLQRALVRVHARAIARLMPALARTLAVPGDLSRRPARIA
                     VVGGGLFPRTALALARVWPAAQVTLLDADAGHLDRARRLLAAHGHAPELLHATWDPHA
                     ASTHDLVIVPLALVGDREAVYAAPGPPRLVHDWSWRRRGHGVVVAWWLLKRLNLVDGT
                     DTRRA"
     gene            complement(1967..2881)
                     /locus_tag="VGB85_31470"
     CDS             complement(1967..2881)
                     /locus_tag="VGB85_31470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HEY0138661.1"
                     /translation="MTCHRLAPVLAALLTLAGVVPSAQASFPAGVWALVEKVTLEPNA
                     DAPNTIRIDGIFMIAKQLPDFAQYPGYGEPQVGYMYYSCAEKNLATCVMEWKELLAVA
                     GTEDNCRGWGEQSLPDNGSVRPAPQPQADPDLYPIAMGVLVGFTPCDALRAWQLDHGT
                     TGESGDTTSTTGDTGTAGESSTTGESASSNATGDTAASTSTATNGDTATGDPQTSGDT
                     GKTATGDPQTTGDASGLGGDTGGDSPTVASAGDSTGGSNSSSSASDSGNLDDDKGCAC
                     TTGPGQNMQGALAMLAALALLRRRRSAA"
     gene            complement(2963..3727)
                     /locus_tag="VGB85_31475"
     CDS             complement(2963..3727)
                     /locus_tag="VGB85_31475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138662.1"
                     /translation="MNALRRVLRAPSLWLALAGLQLLLAAGLAAPLRAVVRVAMGPFT
                     IGDETHILAALLELVRVHPTVIATLVTSVGASAVLGLLLSPLLAGAVIVRLDAPRPVG
                     ELARAAVTHFPAALVIGIYGLVLRVLLALIAAALGTIHPTLQIVALVASLTLCALAVD
                     LARARVVLDGARGLHPRTFVRAVVGASQPGLWLRSGLLTALQWASSLAILLAAVHGIG
                     TAWSPWLVRGLAVLATFVALWRVAVAVDSVAARPRP"
     gene            complement(3724..5832)
                     /locus_tag="VGB85_31480"
     CDS             complement(3724..5832)
                     /locus_tag="VGB85_31480"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEY0138663.1"
                     /translation="MLGSLLALAACGAPAPADSGPPVAEPTHALRPAEVRGTVPAESR
                     VTDYVIDARLDAELHQVRGTVRITWRNRSSRATDSVPLHLYMNGFRAEDTAWMLEARG
                     THRGIRQSDEGRYGYIDLTAARLVGRGREADLHNVEGAAGPAEPLTWKEGEDPSLASV
                     ALPAAVQPGESITLELEFLTQLPEVFARTGYADDFHMLGQWFPKLGVLQPDGSWRAHV
                     FTLNAEFYADFGDYDVHLDVPEAMVVGATGILVGEDPPVDGRKRLHYRAEMVHDFAWA
                     ADPNFIAIDALWKDIRIRQLMHRKRQGDAQRHLEALTAALGSMDQRFGPYPWSTITVV
                     HPPEAAKGAQGMEYPTLFTTSEILHTPAWLDLFGFAERFSGVFTTIHEFGHQYFQGLL
                     ASDEATQPWLDEGLNTTSNMLALTDWHGEDTWFARVGNQTVSVDDFVRGTLDGETQVD
                     PVDSRADTYRAVVGDYGDVVYRKTGALMLTLRRIVGRERFDAAFKAYALEWRFRHPTG
                     DDMVASFLRELGPRVTLADSDAGGEPVVLDLADYFDQALHTVHQVDFSLDQVKNRRRA
                     GTAGWHRDEHGALTLRPTPPEEDLPIAELTDDGIEGLVMVRRIGEFRVPVELEVEFTD
                     GTRERRWWDGQDRYHIFTWPGRRVRSAHIDPDSKLLLEVRRLDDHRAAPEAAPPDGLS
                     RPVGNLGEAAALALLGGLTL"
BASE COUNT          842 a         1907 c         2489 g          805 t
ORIGIN      
        1 ccgcgggtgg tgccggggct cgtgctcgag agcagtcgcg gggcgcctgc cgatgtcggg
       61 ccgcggctcg agccgatcgg cgacgggcgg ctgcgacacc gcggcaaggg cttctcggcg
      121 atcgtcgacg cggacgggag cgtcgagttt tgcgacgtga tcgtcgaggg caagaccagc
      181 gtgctgggct tcgatctgcg cgggaggaag aacccgccgg ccaacctcgc gcgcgacaac
      241 ttcgaggagc gcgcgctgta tcccggcggt ccagcgacgg cgccggtgat ggtcggggtc
      301 ggcggtggtt ttggtggcat cgtcggggcg ttgatctcga aggtgtcgca gcggcgggga
      361 ccgggcaggt cgccggggcg ccggtatacg gccgcgaagc tccagttctt gcgcgacacg
      421 gaggcgatgc ggatgcgcat gacgcatgcg tggctcaagg cgcagctggc gacgcagctc
      481 gatctgctga tcgcgcaggt gctggcagtg tggcgcgaca cgtcggtgcc gctggtcgag
      541 cgcaggcggc ggatcttcgt gatgtgggac gagtgcgagg agccggggcc ggtctcggag
      601 tcggcggtcg agcggatccg cggcgacgcg gcggcgctgg ctcgcacgcg catcgaggcg
      661 ctggtgcggc tgctggcgcc ggccggatcg ccgcgcgcgt atccgcggga cgagctggcg
      721 cggctcaata cgacgcgtcg cagtcgtcgc gccttcgatc cctacgggag ctcaggcgcg
      781 gcgggtgtcg gtgccatcga cgaggttgag ccgcttgagc agccaccacg cgacgacgac
      841 gccgtgcccg cggcgccgcc atgaccagtc gtggaccagg cgcggcgggc cgggggcggc
      901 gtacacggcc tcgcggtcgc cgaccagcgc gagcgggacg atcacgaggt cgtgggtcga
      961 cgcggcgtgg gggtcccagg tggcatgcag cagctcgggt gcgtggccgt gggccgccag
     1021 caggcgtctc gcgcgatcga ggtggccggc gtcggcgtcg agcagggtca cctgcgcggc
     1081 cggccacacg cgggccagcg cgagcgcggt gcgggggaac aggccgccgc cgacgaccgc
     1141 gatccgtgct ggccttcgag acaggtcccc ggggacagcg agggtgcggg ccagcgcggg
     1201 catcaggcgg gcgatcgcgc gggcgtgcac gcggacgagc gcgcgctgca gcggggggca
     1261 ggcgagggcg aggcgctcga gggcaccgag caggcagggg atgctcggcc gggtgacgag
     1321 gaaattgcgc agcaccggcg gaaaacgact gcgtggagcg tcggcgaggc ggcgcgccgg
     1381 caatgtggtc cagtgcgcgc cgggccagcg gtggtgctcg gcgtggtagc cgtcgttgaa
     1441 ccacaggcgg ttgtgcagcg cggcgtcgtg ggagatgccg ccgggtccgg cgaggtgctc
     1501 gccgcggcct tgcagctgac acaggccgag cccgagcaag aagcctggca ggtaggtggc
     1561 gaggaagaag gccggcagcg tgacggcgag ggcgagccac agctgcgcga ccgcggcgat
     1621 ctcgaggctg ccgtaccggc ccgggcgcag cgggccgggg gcctggtcgc ggtgatgcgc
     1681 gaggtggcgg tggcgccaga tcgcctgcgg cacgccggtg agcagcgaga gcagcagcga
     1741 gaacaggcgg ttatatgtgc ggcggcgaaa gaccggggtg tgcaggtgga tatgcgcgac
     1801 ggtgttggac agccaccaca cggtgagcgc gatcgcggct gtcgcaaggg acatgccccc
     1861 aaggccatgt cccgcgagcc agggcaggat cggggcgagc agcgcgaggg cgagcggcgg
     1921 cgcgacgacg gcagcgtcgc gcgggctgtg acgccagggc tgcacgtcag gcggcggacc
     1981 ggcggcggcg caggagggcg agggcggcga gcatggcgag ggcgccctgc atgttctggc
     2041 ccgggccggt ggtgcaggcg cagcccttgt cgtcgtcgag gttgccgctg tcgctggcgc
     2101 tgctgctgct gttgctgccg ccggtcgagt cgccggcgga ggcgacggtc ggggagtcac
     2161 ccccggtgtc gccgccgagg ccggaggcgt cgccggtggt ctgcgggtcg ccggtcgcgg
     2221 tcttgccggt gtcgccggag gtctgcgggt cgccggtcgc ggtgtcgcca ttggtggcgg
     2281 tgctcgtgct cgcggcggtg tcgccggtcg cgttgctcga ggcggactcg ccagtggtgc
     2341 tggactcgcc ggcggtgccg gtgtcgccgg tggtcgaggt cgtatcgccg gactcgccgg
     2401 tggtgccgtg atcgagctgc caggcgcgca gggcgtcaca gggggtgaag ccgacgagca
     2461 cgcccatggc gatcgggtag aggtcggggt cggcctgcgg ctgcggggcg gggcggaccg
     2521 agccgttgtc gggcagcgac tgctcgcccc agccgcggca gttgtcctcg gtgccggcga
     2581 cggcgagcag ctccttccac tccatcacgc aggtggcgag gttcttctcg gcgcacgagt
     2641 aatacatgta tccgacctgc ggctcgccgt agccggggta ctgcgcgaag tccggcagct
     2701 gcttggcgat catgaagatg ccgtcgatgc ggatcgtgtt cggcgcgtcg gcgttgggct
     2761 cgagcgtgac cttctcgacg agcgcccata cacccgcggg gaagctggct tgcgcggacg
     2821 ggacgacacc ggcgagggtc agcagcgcgg cgaggacggg ggcgaggcga tggcaagtca
     2881 tgagccgacg ctaacacagc tggctcggtg cgcatgttcg gcgagcgatc gccgctggac
     2941 ggtgttcgaa caatgaacag gtctacgggc gcgggcgggc ggcgacgctg tcgacggcga
     3001 cggcgacgcg ccacagcgcg acgaaggtgg cgagcacggc gagaccgcgg accagccacg
     3061 gcgaccacgc ggtgccgatg ccgtgcacgg cggcgagcag gatcgccagc gaggaggccc
     3121 actgcagcgc ggtcaacagg cccgatcgca gccacaggcc aggctggctg gcgccgacga
     3181 cggcacgcac gaaggttcgc gggtgcaggc cgcgggcgcc gtcgaggacg acgcgggcgc
     3241 gggcgaggtc gacggcgagg gcgcagagcg tgaggctggc gacgagcgcg acgatctgca
     3301 gcgtcgggtg aatggtgccg agggcggcgg cgatcaacgc cagcagcacg cgcagcacga
     3361 ggccgtagat gccgatcacc agcgcggcgg ggaagtgcgt gacggcggcg cgggcgagct
     3421 cgccgaccgg gcgcggcgcg tcgaggcgca cgatgacggc gccggcgagc agaggggaca
     3481 gcagcaggcc gagcaccgcg ctggcgccga cgctggtgac gagggtggcg atcacggtcg
     3541 ggtggacgcg gacgagctcg agcagcgcgg cgaggatgtg ggtctcgtcg ccgatcgtga
     3601 acgggcccat ggcgacgcgg acgaccgcgc gtagcggcgc ggcgaggccg gcggcgagca
     3661 gcagctgcag gcccgcgagc gccagccaca acgagggggc gcgcagcacc cggcgtagcg
     3721 cgttcacagg gtcagccctc cgagcagcgc gagcgcggcg gcctcgccga ggtttccgac
     3781 ggggcgactg aggccgtcgg gcggcgcggc ctcgggcgcg gcgcgatggt cgtcgaggcg
     3841 gcggacctcg agcagcagct tgctgtcggg gtcgatgtgg gccgatcgca cgcgtcgacc
     3901 aggccaggtg aagatgtgat agcggtcctg gccgtcccac cacctgcgct cgcgggtgcc
     3961 atcggtgaac tcgacctcga gctcgaccgg cacgcgaaac tcgccgatcc ggcggaccat
     4021 gacgaggccc tcgatgccgt cgtcggtgag ctcggcgatc gggaggtcct cctcgggcgg
     4081 ggtcggacgc agggtcagcg cgccgtgctc gtcgcggtgc cagccggccg tgccggcgcg
     4141 gcggcggttc ttgacctggt cgaggctgaa gtcgacctga tgcacggtgt gcagcgcctg
     4201 gtcgaagtag tcggcgaggt cgagcacgac cggctcgccg ccggcgtcgc tgtccgcgag
     4261 ggtgacgcgg ggaccgagct cgcgcaggaa gctggcgacc atgtcgtcgc cggtcgggtg
     4321 gcgaaaccgc cactcgaggg cataggcctt gaaggcggcg tcgaagcgct cgcggccgac
     4381 gatccggcgc agggtcagca tcagggcgcc ggtcttgcga tagacgacgt ctccgtagtc
     4441 gccgacgacg gcgcggtaag tgtcggcgcg cgagtcgacg gggtcgacct gggtctcgcc
     4501 gtcgagggtg ccgcggacga agtcgtcgac gctgacggtt tggttgccga cccttgcgaa
     4561 ccaggtgtcc tcgccgtgcc agtcggtgag ggcgagcatg ttgctggtgg tgttgaggcc
     4621 ctcgtcgagc cacggctgcg tggcctcgtc ggaggcgagc aggccctgga agtactggtg
     4681 gccgaactcg tggatcgtcg tgaacacgcc gctaaagcgc tcggcgaagc cgaacaggtc
     4741 gagccaggcg ggcgtgtgca ggatctccga ggtcgtgaag agggtcgggt attccatgcc
     4801 ctgcgcgccc ttggcggcct cgggcgggtg cacgacggtg atcgtcgacc aggggtaggg
     4861 gccaaagcgc tggtccatgc tcccgagcgc ggccgtcagc gcctcgaggt ggcgctgcgc
     4921 gtcgccctgg cgctttcggt gcatcagctg gcggatccgg atgtccttcc acagcgcgtc
     4981 gatcgcgatg aagttcgggt cggccgccca cgcgaagtcg tggaccatct cggcgcggta
     5041 gtgcaggcgc ttgcgaccgt cgaccggcgg gtcctcgccg acgaggatcc cggtcgcgcc
     5101 gacgaccatc gcctccggga cgtcgaggtg gacgtcgtag tcgccgaagt cggcgtagaa
     5161 ctcggcgttg agcgtgaata cgtgcgcccg ccagctgccg tcgggctgca acacgccgag
     5221 cttggggaac cactgcccga gcatgtggaa gtcgtcggcg tagccggtgc gcgcgaacac
     5281 ctccgggagc tgggtcagga actcgagctc gagcgtgatg ctctcgcccg gctgcaccgc
     5341 cgcgggcagc gcgacgctgg cgagcgaggg gtcctcgccc tccttccagg tcagcggctc
     5401 ggccggaccg gcggcgccct cgacgttgtg caggtcggcc tcgcgtccgc ggccgaccag
     5461 gcgcgcggcg gtcaggtcga tgtagccgta gcggccctcg tcgctctggc ggatgccgcg
     5521 atgcgtaccc cgggcctcga gcatccacgc ggtgtcctcg gcgcgaaagc cgttcatata
     5581 aaggtggagc ggcacgctgt cggtcgcgcg gctgctgcgg ttacgccagg tgatccgcac
     5641 ggtgccgcgg acctggtgga gctcggcgtc gaggcgcgcg tcgatcacgt agtcggtgac
     5701 acgcgactcg gcgggcacgg tgcctcgcac ctccgcgggg cgcagggcgt gcgtcggctc
     5761 ggcgaccggc ggaccgctgt ccgcgggcgc aggcgccccg cacgcggcca aagccagaag
     5821 agacccgagc aaagacccga gcagagacgc aagcgccgtg cggcgggtgg cggtccggtc
     5881 gaccatcgga ctgcatgcct accatctgtg tttcgcgggg cacaagccag gcccgcgtgt
     5941 atagtccggc gccgtgaggg ccgggccgat cgacgaggct acgctggtga tgcgcgcacg
     6001 cgcactcggg gtcgcgctgc gccagcaaga gcgcctcgcg ggg
//
LOCUS       DASWNJ010001890         3775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_527510, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001890 DASWNJ010000000
VERSION     DASWNJ010001890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527510"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /locus_tag="VGB85_31485"
     CDS             complement(<1..1288)
                     /locus_tag="VGB85_31485"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HEY0138664.1"
                     /translation="MQKLLAAVHAAASPRDWSRGVELSRAGAVLSKRTADDEIVVQVA
                     VKGAAVSPTVTLWPADAEWSCDCDSRADPCFHVAAAVIALKRAAEAGEALPTATARAG
                     RVGYRFTRSAEGLVFTRVRVAEGTSETPLTHSLALLSSGRIEGPSTVASQADMAVDLA
                     LGAFNKSGVMPVPLARKVMPLLAACSDIRLDGEPIQIDAKPVGLHARVADDPGGFRIA
                     LTRDPAITENFGGDIFRCGECLRVGQPPVLPEAWAALKKGPMVVSRERATWLVTEMLP
                     ALERAIPVKVTAKELPRLSDGYAPHVVLEVAQSGEALTVLPTIVYGKPPVARLDGERI
                     VPFGRVLPRRDLEAEGRLRRMLAKHSLAAGERNAAVAGAAVELGERVRGLAKAGRDSV
                     ELRGEAHQGFVRLGPLVPRMGPGGGLDLSFETEGGRR"
     gene            complement(1347..2171)
                     /locus_tag="VGB85_31490"
     CDS             complement(1347..2171)
                     /locus_tag="VGB85_31490"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEY0138665.1"
                     /translation="MTTHPDPTSNSATEILRERLGGRKIDWAVVAGSGLGVGFVSPTD
                     GIGLQIEGQIQLAELGLPAPSVAGHGNSLVWGRVGDSTVCVQTGRLHPYEGHPMAVCT
                     APLLAMIDCGARAAALTFAVGGLDPALRTGEIVLLRDQIALFGPTPLVGARFVDCSRI
                     YPRHLRQRVQQIHPGMGATQLPEVVYAHARGPQYETPAETEALRRLGGQVVGMSTTYE
                     AVLCAAHGVPVLGLGVVTNGAGQTGLSHLEVQERSNEARAQIAALLGAVLAGSPDA"
     gene            complement(2274..3329)
                     /locus_tag="VGB85_31495"
     CDS             complement(2274..3329)
                     /locus_tag="VGB85_31495"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0138666.1"
                     /translation="MSVPTQIGPFKVKGLLGKGAMGEVYEGIDEKLGRRVAIKILGRK
                     HRESEEFKTRFLREGRALAAMNHPNIVAVYFIGEHEDRPFLAMEFLDGEDIGGSLKKR
                     GALNPGDAAEVIRQAAIGLAETQRVGVVHRDVKPSNLVVTSQGFVKVTDFGLAKALQE
                     DLSITATGVFVGTPDYLAPEQAMGKEVDARADVYALGCSLYHMCSGHPPFRKGGQDDH
                     YTAVVRRHLKSERPELKLEIKGFDEPLSDLCRRMMSRRPDVRPTFEELKTSLSEISRR
                     LGGQVPPRHSSVELRSPEEISEASKKTGAKATELVPGGGGGDNVPARSTRTTMLAVGV
                     GVGIGIALGVVLAIWSL"
     gene            complement(3580..>3775)
                     /locus_tag="VGB85_31500"
     CDS             complement(3580..>3775)
                     /locus_tag="VGB85_31500"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0138667.1"
                     /translation="IMYIYREERYLPGDASPADRQKVENKAEIILGKQRNGPIGTVHL
                     TFLKHHTRFENMLNGPGITD"
BASE COUNT          531 a         1362 c         1349 g          533 t
ORIGIN      
        1 cgcgccggcc accctctgtc tcgaaggaca ggtcgaggcc gccgcccggg cccatgcgcg
       61 ggaccagcgg gccgaggcgc acgaagccct ggtgggcctc gccgcgcagc tcgactgaat
      121 cgcggccggc cttcgccaat ccgcgcacgc gctcaccgag ctcgaccgcc gcgccggcga
      181 ccgccgcgtt gcgctcgccg gccgcgagcg agtgcttcgc caacattctc cgtaatcgcc
      241 cctcggcctc gaggtcgcgc cgcggcagca cgcgcccgaa gggcacgatg cgctcgccgt
      301 cgaggcgggc caccggcggc ttgccataga cgatcgtcgg cagcaccgtc agcgcctcgc
      361 cgctctgggc cacctcgagc accacgtgcg gcgcataccc gtcgctgagc cgcggcagct
      421 ccttcgcggt caccttgacg gggatcgcac gctcgagcgc gggcagcatc tcggtgacca
      481 gccaggtcgc ccgctcgcgc gagaccacca tgggtccctt tttcagcgcg gcccaggcct
      541 ccggcagcac cggcggctgc cccacacgca ggcattcgcc gcagcgaaag atgtcgccgc
      601 cgaagttctc cgtgattgcg ggatcgcggg tcagcgcgat gcgaaagcct cccgggtcat
      661 ccgcgacgcg cgcgtgcagg ccgaccggct ttgcatcaat ctggatcggc tcgccgtcga
      721 ggcggatgtc cgagcacgcc gcaagcaagg gcatgacttt gcgggccagc ggcaccggca
      781 tcacgccgga tttattaaag gccccgagcg cgaggtcgac ggccatgtct gcctgggacg
      841 cgaccgtcga cgggccctcg atgcgaccgg agctcagcag cgcgaggctg tgtgtcagcg
      901 gcgtctcgct ggtgccctcg gccacgcgca cgcgcgtgaa caccagcccc tccgcgctgc
      961 gcgtgaagcg atagccgact ctgcccgccc gggccgtcgc ggtcggcagc gcctcgccgg
     1021 cctcggccgc ccgcttgagg gcgatcacag cggcggcgac gtggaagcag gggtccgcgc
     1081 ggctgtcaca atcgcagctc cactcggcgt ccgccggcca cagcgtgacc gtcgggctga
     1141 ccgcggcgcc cttgaccgcc acctgcacga cgatctcgtc gtcggcggtg cgcttgctga
     1201 gcaccgcgcc ggcgcgcgac agctccaccc cgcgcgacca gtcgcgtggg ctcgcggcgg
     1261 cgtgcacggc ggcgagcaac ttctgcacgc cgaggggcta gcacgccagg cacgcgctca
     1321 caacctgccc cgagggacag ccgggctcag gcgtcgggcg agcccgcgag caccgcgccg
     1381 agcagcgctg cgatctgcgc gcgcgcctcg ttgctgcgct cctggacctc gaggtggctc
     1441 aggcccgtct ggccggcccc gttggtgacg acgccgaggc cgagcaccgg cacgccatgg
     1501 gccgcgcaca ggaccgcctc gtaggtggtc gacatgccga cgacctgccc gccgagccgg
     1561 cgcagcgctt cggtctcggc cggcgtttcg tactgcgggc cgcgggcgtg cgcgtagacg
     1621 acctcgggca attgcgtcgc gcccatgccc gggtggatct gctgcacgcg ctgacgcagg
     1681 tgccgcgggt agatccggct gcaatccacg aacctcgcgc cgaccaaggg cgtcggtccg
     1741 aacagggcga tctggtcgcg cagcagcacg atctcgccgg tgcgcagcgc cgggtcgagg
     1801 ccgccgaccg cgaaggtcag ggccgccgca cgcgcgccgc agtcgatcat cgccagcaaa
     1861 ggcgccgtgc acacggccat cgggtggccc tcgtaggggt gcaggcggcc ggtctgcacg
     1921 cacaccgtcg agtcaccgac gcggccccac accaggctgt tgccgtggcc ggcgaccgag
     1981 ggcgcgggca ggccgagctc ggcgagctgg atctggccct cgatctggag gccgatgccg
     2041 tcggtcggcg agacgaagcc gacgccgagc cccgagccgg cgacgaccgc ccagtcgatc
     2101 ttgcgaccgc cgaggcgctc gcgcaggatc tcggtggcag aattggaggt gggatcggga
     2161 tgcgtggtca tgcggagctc gggcccggcc aaggggcgaa ggggggccgg ccaaggggtg
     2221 ccggcgaagg ggtgccggcg aaggggtgcc gactaaacag gcacagacgc gggtcagagc
     2281 gaccagatcg cgaggacgac gccgagggcg atgccgatgc cgacgccgac gccgaccgcg
     2341 agcatggtcg tgcgggtcga gcgggccggc acgttgtcac caccaccgcc gccggggacc
     2401 agctcggtcg ccttggcccc cgtcttcttg gaggcctcgc tgatctcctc ggggctgcgc
     2461 agctcgacgc tgctgtggcg cgggggcacc tgaccgccga ggcggcgcga gatctcggag
     2521 aggctggtct tgagctcctc gaaggtcggg cgcacgtccg gacgacgact catcatccgg
     2581 cgacacaggt ccgagagcgg ctcgtcgaag cccttgatct cgagcttgag ctcggggcgc
     2641 tccgacttga ggtggcggcg cacgaccgcg gtgtagtggt cgtcctggcc gcccttgcga
     2701 aacggcgggt ggcccgagca catgtgatag agcgagcagc cgagcgcgta cacgtcggcg
     2761 cgcgcgtcga cctccttgcc catcgcctgc tccggcgcga ggtagtcggg cgtgccgacg
     2821 aacacgccgg tcgcggtgat cgagaggtcc tcctgcagcg ccttggcgag gccgaagtcg
     2881 gtcaccttca cgaagccttg cgaggtgacg acgaggttcg acggtttgac gtcgcggtgg
     2941 acgacgccga cgcgctgggt ctcggcgagg ccgatcgcgg cctggcggat gacctcggcc
     3001 gcatctccgg ggttcaaggc gccgcgcttc ttgagcgagc cgccgatgtc ctcgccgtcg
     3061 aggaactcca tcgccaaaaa cggccggtcc tcgtgctcgc cgatgaagta caccgcgacg
     3121 atgttcgggt ggttcatcgc cgcgagggct cgaccctcgc gcaggaagcg ggtcttgaac
     3181 tcctcggatt cccggtgctt tcgcccgagg atcttgatcg ccacgcggcg gccgagcttc
     3241 tcgtcgatgc cctcgtagac ctcgcccatg gcccccttgc cgaggagacc cttgaccttg
     3301 aacggcccga tctgggtggg caccgacatc gccccgcaag ctatcacggc cagcgccggc
     3361 ttcgccacgc ttgcgcgcgg gccgagcgtc tttgccggag ccggccgtcg gccgccgcga
     3421 gccctcgccg ggccctcggg acaggtgagg acggccgcag cgccccggcc ctcgccggcc
     3481 cgtgatggcc ccggaaaata ccgcggaaca gtcctcggcg gcccgtgacg ccgcttcgcg
     3541 gcagaccttg cggcactttg ggccgcggcg accgtcgcct cagtccgtga tccccgggcc
     3601 gttcaacatg ttctcgaagc gggtgtgatg cttcaggaag gtcaggtgca ccgtcccgat
     3661 cgggccgttg cgctgcttgc cgaggatgat ctcggccttg ttctcgacct tttgccggtc
     3721 ggccggcgag gcgtccccgg gcaggtagcg ctcctcccgg tagatgtaca tgatg
//
LOCUS       DASWNJ010001891         4027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_173297, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001891 DASWNJ010000000
VERSION     DASWNJ010001891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173297"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            489..3266
                     /locus_tag="VGB85_31505"
     CDS             489..3266
                     /locus_tag="VGB85_31505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138668.1"
                     /translation="MKRWKLSVSSVAVVCAFGGFVGPAQAAPAFTAFESGQVRPLALS
                     PDSTRLFAVNTPDNRLEVYKVTKHGLQHEASVPVGLEPIAVAARSNDEVWVVNHLSDS
                     VSVVDLDGTNARVTRTLLVGDEPRDIVFAGPGKSRAFITTAHRGQNAPFNPQNTTPGV
                     GRADVWVFAVNNLGSGLGGTPLTIVNLFSDIPRALAASPDGSKVYAAAFHSGNKTAVV
                     PLNQISDDGEAAGGIPLPNVNHAGVSEPNLSLIVKFDGQHWVDETGRSWDAHIKFNLP
                     DKDVFVINANAGTPALVAGNAGVFKGVGTTLFNMIVNPKNGKVYVTNTEARSHVRFEG
                     HGDFAETTVRGHLVDTRITVLDGQGVHPRLLNKHIDYDDCCDEVPNDENALSISQPIG
                     MAITPDGKTLYTAVLGNDKVVIHDTKELETDSFFPDEVDQISVTGGGPTGLVLDHWRE
                     RLYVMTRFDNGISIIDTDSRDEIGHVTMHNPEPPEIVEGRRFLYDAALTSSHGDSSCA
                     TCHIFGDNDQLAWDLGNPDGDVTPFPDVVRAEFPFGDTAFHPNKGPMTTQSLRGMANH
                     GPMHWRGDRNGQGQGPNVQPDGGAWSEEAAFKAFNPAFVELLGRDEELSPAQMQAFTD
                     FTLRLMYPPNPHRGLDDSLNADQEAGRQFFTSKKSLLVGPNSPVSGVGFACVDCHVID
                     RQANAQYGVEFPGFFGSSGHVVPGEFSQTFKIAHFRNAYTKIGAFGFAADPNFNVPGM
                     LDYDISHTGDQIRAFGYTHDGSNDTIMRFVNAFSGPLSPEPFASAAQQKQVAEFIFAF
                     DSNLRPVVGQQITLTGSNAGVAGPRIDLFKARHAAGDCELIAKTDIVGHELGFAYTGT
                     SFKTSFAGAQNISDTQLRQYAQTYGQPVTFTCVPPGSGLRLGVDRDADGHRDGDELFA
                     GSDPADPNSTP"
     gene            3419..>4027
                     /locus_tag="VGB85_31510"
     CDS             3419..>4027
                     /locus_tag="VGB85_31510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138669.1"
                     /translation="MTQPTIQVDQVVFSWDLERGLMLCWGQPVVCWWIESTMAGFMAG
                     IQRMVGTERFNLALEGAGRESTEGEWHNIILKQPSVEEGLAFIGQASRLVGLGEWELV
                     ELDREAQRMRFRARNSWEGMYQRAMGVSWGTASLAGKLAAYSERTLGVPCSIEQTAFI
                     ARGDEFDEFTATASPTTFMRRLEQVVDSDHATRQDLADALARV"
BASE COUNT          682 a         1387 c         1363 g          595 t
ORIGIN      
        1 ggcttcggcc tcggcctggc gatcgccaag caggccgtcg acgcccacgc cggcacgatc
       61 cgcgtgcaga acttgccagg caagggctgc atcttcgtgc tcgagttgcc gctctcgggc
      121 cgtccgaccg cgacctgagt cgtcgcttcc tgcgcacttc gcgggggcga gcgatctccc
      181 gggcgacgat cgctgtgcct cggcgcaggt acgattccgc gtcccggagg ccgcgctcgc
      241 cgcacacccg gcgccggtcg agaatgtggg tacattcctg actcgcgcca cgccggccac
      301 acgaacgctg taagcgaggc cgtctgcaga tgcgagggtg ggttcatgtc gcggcgaggt
      361 gcacctggcc tgcctgcgtg actgtggata tatgcgccgc agggcccaca tcgaaggccg
      421 cggcgccgac ggccgggacc cggggaccac agctattcgc gtggcgatcg cgtcgatacg
      481 gtcgatgcat gaagcgctgg aaactgagtg tgtcgtcggt cgccgtggtg tgtgcattcg
      541 gggggttcgt ggggccggcc caggcggcgc ctgcgttcac cgccttcgag agcggccagg
      601 tccggccgct cgcgctgtcg cccgacagta cgcggctgtt cgccgtcaac acccccgata
      661 accgcctcga ggtctacaag gtcacgaagc acggtctgca gcacgaggcg tcggtgccgg
      721 tcgggctcga gccgatcgcc gtcgcggcgc gcagcaacga cgaggtctgg gtcgtcaacc
      781 acctctcgga cagcgtcagc gtcgtcgacc tcgacggcac gaacgcccgg gtgacgcgca
      841 cgctgctcgt cggcgacgag ccgcgcgaca tcgtgttcgc cggaccgggc aagtcgcggg
      901 cgttcatcac gacggcgcac cgcgggcaga acgcgccgtt caacccgcag aacacgacgc
      961 cgggggtcgg tcgcgcggac gtgtgggtgt tcgcggtcaa caacctcggc agcgggctcg
     1021 gcgggacgcc gctgacgatc gtcaatctct tcagcgacat cccgcgggcg ctcgccgcct
     1081 cgccggacgg gagcaaggtc tacgccgcgg cgtttcactc cggcaacaag accgcggtcg
     1141 tgccgctcaa ccagatcagc gacgacggtg aggcggccgg cggcatcccg ctgccgaacg
     1201 tcaaccatgc cggcgtcagc gagcccaacc tgagcctgat cgtcaagttc gacggtcagc
     1261 actgggtcga cgagaccggc cgcagctggg acgcgcacat caagttcaac ctgcccgaca
     1321 aggacgtgtt cgtgatcaac gccaacgccg gcaccccggc gctggtcgcc ggtaacgccg
     1381 gggtgttcaa gggcgtcggc accaccctct tcaacatgat cgtcaacccg aagaacggga
     1441 aggtctacgt caccaacacc gaggcgcgca gccacgtgcg cttcgagggc cacggcgact
     1501 tcgcggagac gaccgtgcgc ggccacctcg tcgacacgcg gatcacggtg ctcgacggtc
     1561 agggcgtgca cccgcggctg ctcaacaagc acatcgacta cgacgattgc tgcgacgagg
     1621 tgccgaacga cgagaacgcg ctctcgatct ctcagccgat cggcatggcg atcacgccgg
     1681 acgggaagac gctgtatacc gcggtgctcg gcaacgacaa ggtcgtgatc cacgacacca
     1741 aggagctcga gacggacagc ttcttcccgg acgaggtcga ccagatctcc gtcaccggcg
     1801 gcggcccgac cggcctggtg ctcgaccact ggcgcgagcg cctgtatgtg atgacgcgct
     1861 tcgacaacgg catctcgatc atcgacaccg atagccgcga cgagatcggc cacgtgacga
     1921 tgcataaccc cgagccgccg gagatcgtcg agggccggcg cttcctgtac gacgcggcgc
     1981 tgacctcgag ccacggcgac tcgtcgtgcg cgacctgcca tatcttcggc gacaacgacc
     2041 agctcgcctg ggacctcggc aaccccgacg gtgacgtcac gccgttcccc gacgtcgtgc
     2101 gcgccgagtt cccgttcggc gacaccgcgt ttcacccgaa caaggggccg atgacgaccc
     2161 agagcctgcg cggcatggcg aaccacggcc cgatgcactg gcgcggcgat cgcaacggtc
     2221 aggggcaggg gcccaacgtg cagccggacg gcggcgcgtg gagcgaggag gccgcgttca
     2281 aggccttcaa ccccgccttc gtcgagctgc tcggccgcga cgaggagctg agcccggcgc
     2341 agatgcaggc gttcaccgac ttcacgctgc gcctgatgta tccgcccaac ccgcaccgcg
     2401 ggctcgacga ctcgctcaac gccgaccagg aggcgggccg tcagttcttc acgagcaaga
     2461 agtcgctgct cgtcggtccg aactcgccgg tgtccggcgt cgggttcgcg tgcgtggatt
     2521 gccacgtgat cgaccgccag gccaacgcgc agtacggggt cgagtttccc ggcttcttcg
     2581 gcagcagcgg tcacgtggtg cccggtgaat tctcgcagac cttcaagatc gcccactttc
     2641 gcaacgccta caccaagatc ggcgccttcg gcttcgccgc ggacccgaac ttcaacgtgc
     2701 cgggcatgct ggactacgac atcagccaca cgggcgatca gatccgtgcc ttcggataca
     2761 cgcacgacgg ctcgaacgac acgatcatgc gcttcgtcaa cgcgttctcg ggcccgctct
     2821 cgcccgagcc gttcgccagc gcggcgcagc agaagcaggt cgccgagttc atcttcgcct
     2881 tcgacagcaa cctgcggccg gtcgtcggcc agcagatcac gctgaccggc agcaacgcgg
     2941 gggtcgcggg cccgcggatc gacctgttca aggcgcgtca cgccgccggc gactgcgagc
     3001 tgatcgccaa gaccgacatc gtcgggcacg agctcggctt cgcctacacc ggcacgagct
     3061 tcaagaccag cttcgccggc gcgcagaaca tcagcgatac gcagctgcgg cagtatgcgc
     3121 agacttatgg acagccggtg accttcacct gcgtcccgcc gggctcgggc ctgcgcctcg
     3181 gcgtcgatcg tgacgccgat ggccatcgcg acggcgacga gctgttcgcc ggcagcgacc
     3241 cggccgaccc gaacagcacg ccgtgacgac cgacccgcgt ggggtcatga cagcctcacc
     3301 gcgcttcccg atcgggggcg cgcgggagcg gggacggggt acgagcaggt aagcagatac
     3361 ggacgccgcc aggattgcgt tgttccgacc aaggctgatc tggtacgaca ggaatgcgat
     3421 gacgcagccg acgattcaag tggaccaggt cgtgttctcc tgggacctcg agcggggcct
     3481 gatgctgtgc tgggggcagc cggtcgtgtg ctggtggatc gagtcgacga tggcgggctt
     3541 catggccggc atccagcgca tggtcggcac cgagcgcttc aacctcgccc tcgagggcgc
     3601 cgggcgcgag agcaccgagg gcgagtggca caacatcatc ctcaagcagc cctccgtcga
     3661 ggagggcctg gcgttcatcg gccaggcgag tcgcctcgtc gggctcggcg agtgggagct
     3721 cgtcgagctc gaccgcgagg cccagcgcat gcggttccgg gcgcgcaaca gctgggaagg
     3781 catgtatcag cgcgcgatgg gcgtcagctg gggcaccgct tcactggccg ggaaattggc
     3841 ggcctacagc gagcgcacgc tgggggtgcc gtgcagcatc gagcagaccg ccttcatcgc
     3901 gcgcggggat gagttcgacg agttcacggc gacagcctcg ccgacgactt ttatgcggcg
     3961 tctcgagcag gtcgtcgaca gcgaccacgc gacgcgccag gacctggccg acgcgctcgc
     4021 gcgtgtg
//
LOCUS       DASWNJ010001892         2113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_455945, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001892 DASWNJ010000000
VERSION     DASWNJ010001892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455945"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="VGB85_31515"
     CDS             complement(<1..928)
                     /locus_tag="VGB85_31515"
                     /inference="COORDINATES: protein motif:HMM:NF020367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase family 7 protein"
                     /protein_id="HEY0138670.1"
                     /translation="MRPVLRSPLMLVLLAACTTEPGVTTGGSTGGSTSATTSTDSTAA
                     PTGSETSPTTGEPATGDTPTSQSPTGDTSTGGTGDTSTGDPGTDTSTGAPAMLPGDVL
                     DLQNWKLTLPVDTDHPGNPDEIEQPELAEFVDPLHYHLSDELDGVVFRAHAGGVTTEG
                     SGYPRSELREMTDAGAEEAAWSSGTGKHRMFIDQRIVHLPVEKPHIVVGQIHDDQDDV
                     IVFRLEGSKLFIDLNGDDGPVLTDDYVLGDRFTVAFEVSDDHVDCYYNDALVYTHEQA
                     FSGAYFKAGAYVQSSCQGDKKVPDESCEAFGEV"
     gene            1033..1944
                     /locus_tag="VGB85_31520"
     CDS             1033..1944
                     /locus_tag="VGB85_31520"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY0138671.1"
                     /translation="MRGAGASVLGRVLGLHVLLVPACADVGTASTSETSATSSSSGTT
                     SPTSGGDEAATTGTADTTGEGGSTSSGSTGAEDGTTGGSSSGEPPPAAVTVSVLGAST
                     AVGWNLDKPEYGGEVGGLKYSWVNRYEAYLQVERPGSQVYNLSKAGYGTYEALPTGTV
                     NPADRPQVDPVRNITAALARDPDVIIVHYPSGGDLDNGILVKEIIDNLEVIAATAAAA
                     NVPIWVATPNPLSKTPQAKIDQFLDMRAQILTIYGDRALDFWALRVGPDGNALPEYTL
                     LDGHHPNAEGHRLLFELVVAVKVVAPV"
     gene            complement(1922..>2113)
                     /locus_tag="VGB85_31525"
     CDS             complement(1922..>2113)
                     /locus_tag="VGB85_31525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138672.1"
                     /translation="GFAGLAAAGWAVERTWGSANPMRPAVDLVFDHAALLIAALAVVS
                     LIVLARDLLSRTPKQAPRP"
BASE COUNT          320 a          669 c          796 g          328 t
ORIGIN      
        1 cgacctcgcc gaacgcctcg caggactcgt cggggacctt tttgtcgccc tggcaggacg
       61 actgcacgta cgcgccggcc ttgaagtagg cgccggagaa cgcctgctcg tgggtgtaga
      121 cgagcgcgtc attataatag cagtcgacgt ggtcgtcgct gacctcgaag gcgacggtga
      181 agcggtcgcc gagcacatag tcgtcggtca gcacggggcc gtcgtcgccg ttgaggtcga
      241 tgaacagctt gctgccctcg aggcggaaga cgatcacgtc gtcctggtcg tcgtggatct
      301 ggccgacgac gatgtgcggc ttctcgaccg gcaggtgcac gatccgctga tcgatgaaca
      361 tgcggtgctt gccggtgccg gacgaccacg cggcctcctc ggcgccggcg tccgtcatct
      421 cgcgcagctc ggagcgcggg tagcccgaac cctcggtggt cacgccgccc gcgtgcgcgc
      481 ggaacacgac gccgtcgagc tcgtcgctca aatggtagtg gagcgggtcg acgaattcgg
      541 cgagctcggg ctgctcgatc tcgtcggggt tgcccgggtg gtcggtgtcg accggcaggg
      601 tcaacttcca gttttgcagg tcgagcacgt cgccgggcaa catcgccggc gcgccggtgc
      661 tggtgtcggt gccggggtcg ccggtgctcg tgtcgccggt gccgcctgtg ctggtgtcac
      721 cggtcggaga ttgcgaggtc ggggtgtcac cggtggcggg ctcgccggtg gtcggcgagg
      781 tctcgctgcc ggtcggcgct gcggtcgagt ccgtgctcgt ggtcgcggag gtcgagccgc
      841 ccgtggagcc cccggtggtg acgccaggct cggtggtgca ggcggccagc aggacgagca
      901 tgaggggcga gcggagcacg gggcgcatcg ccgcagcata caggccggcg ggtgcggagg
      961 tcacccgtgg cgtgcacggg cgcggtcgcg cgggcgatgt gcttgtgtcg cggtctcgcg
     1021 tagagtggct ggatgcgtgg agcaggagcg agcgtcctcg gccgggtcct tggcctgcat
     1081 gtgctgctcg tgccggcctg cgccgatgtc gggacggcga gcacgagcga gacctccgcg
     1141 acgtcgtcga gcagcggcac gacgagcccg acctcggggg gcgacgaggc ggcgacgacc
     1201 ggcaccgcgg acacgacggg cgagggcggg agcacgtcga gcggctcgac gggggccgag
     1261 gatggcacca cgggcggcag cagcagtggc gagccaccgc cggctgcggt caccgtctcg
     1321 gtgctcgggg cgtcgacggc ggtcggatgg aacctcgaca agcctgaata tggaggtgag
     1381 gtcggcgggc tcaagtacag ctgggtcaac cgctacgagg catatttgca ggtcgagcgg
     1441 cccggctcgc aggtatataa tttgtcgaag gccggctacg gcacctacga ggcgctgccg
     1501 acaggtacgg tcaatcccgc cgaccggccg caggtcgatc cggtccgtaa catcaccgcc
     1561 gcgctcgcca gggaccccga tgtcatcatc gtgcattatc cctccggcgg agatctcgac
     1621 aacggcattc tcgtaaagga gatcatcgac aacctcgagg tgatcgcggc caccgcggcc
     1681 gccgcgaacg tgccgatctg ggtcgccacg ccgaaccccc tgtcaaaaac accgcaggcg
     1741 aagatcgatc agttcctcga catgcgcgcg cagatcctga ccatttatgg cgatcgtgcg
     1801 ctcgacttct gggcgctgcg cgtcggcccc gacggcaacg cgctgccgga atatacgctg
     1861 ctcgacggcc accacccgaa cgccgagggg caccggttgt tgttcgagct ggtggtggcc
     1921 gtcaaggtcg tggcgcctgt ttaggcgtcc gcgacagcag gtcacgggcg agcacgatca
     1981 gcgagacgac ggcgagcgcg gcgatgagga gcgcggcgtg gtcgaagacg aggtcgacgg
     2041 cgggtcgcat cgggttggcg gacccccacg tgcgctccac cgcccagccg gcggcggcga
     2101 ggcccgcgaa gcc
//
LOCUS       DASWNJ010001893         3788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_527552, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001893 DASWNJ010000000
VERSION     DASWNJ010001893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527552"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VGB85_31530"
     CDS             complement(<1..377)
                     /locus_tag="VGB85_31530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEY0138673.1"
                     /translation="MISVRLVVGSFHISLAFAPVFAVMTAFLDVGCHKESSKAPVDDG
                     PCTGARCVEEAEAAMYYKDYAKAREPLATVCEKDGFACFRLAELHQHGRGGPVDLTKA
                     AALYEESCAKEYGEGCERRYIMA"
     gene            complement(487..2031)
                     /locus_tag="VGB85_31535"
     CDS             complement(487..2031)
                     /locus_tag="VGB85_31535"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEY0138674.1"
                     /translation="MLTGLVGCGGAGSTAETAETSTTSGTSASTSGTPTTGGSVTDGP
                     CAEPIDPVERSHARLRVDTMGVLRDGLGRDIQLRGVNAGGRSKWAPFMPFPMSEDASL
                     DEVKAGAAAFFAPLPGWGLDTVRVPFSWEALEPVEGMYDERYLDRYVAMVDAAWALGL
                     RVIVDFHQDVYASPFCGDGFPLWTISGEVGPPRRDCPDWGLGYIIDPGVREAFDRFWA
                     DEGGVQGKYRKMWEKMASRVATHPGVLALELINEPGWGTAPNINAWKASTLTPFHTQM
                     IAHMRGVVGDELLLVFNNTGVEAAGLSPTVHMRPEGTNLMYGPHMYDGGLVGGELYAG
                     HEPEVHMASLAEFSRAEGVAPLIGEFGYNIVADEAGTAGASWLTRVVDALDAERVSGT
                     LWEYSRNEALWNEEDLSLVDAAGVPRPILDVYVRPYLRAVAGGSPSFHWDVATGTGVA
                     RWVGDGGVTEVVVPVRSFPDGLSELSLETEAGAPGSCYYHDAARGELRVRVTPGAQVE
                     LRFKRE"
     gene            complement(2036..3253)
                     /locus_tag="VGB85_31540"
     CDS             complement(2036..3253)
                     /locus_tag="VGB85_31540"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 family protein"
                     /protein_id="HEY0138675.1"
                     /translation="MNARDLVMILGLSLVACGDDGGAGETGGTSTGEASATTGAGATS
                     TGGEQPTGSSTTSSTSTSSTGDTPTTGEPGPGMTTGADTMMDTTGAPVDEWQEFLDGR
                     EDWLRALAVPISACVAMNDTEHPVFNGCIDWHSAVHATYALHAVYRHTGDMSYLEAAE
                     AKLTDVGLAAELLRIQQGLLPQEVPYGYAWFLTLARERELSTGKKDLRPLAMEIRGQL
                     REWVDSRTPMELEAGALADDYNSLTWAVLNLWQWAEFDNDPELAGAMESLALNTLQAP
                     GLDASCPFSQEEADADDFFPPCLHRARLLVNLLPADAQATWLAGWLPAIPALTPIDMP
                     AKAHISGLNFSRAWGLWSLYEATDDIAYRDLFLDHMITHLEHPQYWAEDYGNYAHWVA
                     QFGVYAISQTYPQ"
BASE COUNT          554 a         1453 c         1211 g          570 t
ORIGIN      
        1 tgcgccatta tatagcggcg ctcgcagccc tcgccgtact ccttggcgca cgactcctcg
       61 tacagcgccg ccgccttcgt caggtcgacc ggaccgccgc ggccgtgctg atgcagctcc
      121 gccagccgga agcacgcgaa gccatccttc tcacagacgg tcgccagcgg ctcgcgtgcc
      181 ttcgcataat ccttgtaata catcgccgcc tcggcctcct cgacgcagcg agcgccggtg
      241 cacggcccgt cgtcgaccgg cgccttcgag gattccttgt gacagccgac gtcgaggaac
      301 gcggtcataa ccgcgaacac cggcgcaaac gcgagggaaa tgtgaaatga gccgactacg
      361 aggcgcacgg agatcatgga agacgagggg acctccgagg tcaaccctaa tttctgcgcg
      421 gacaaccgtc gcagcgtccg cgcgcgcgcg caggccgccc gcgcacgagc gacgtcttca
      481 cgcgcatcac tctcgcttga agcgcagctc gacctgcgcg ccgggagtga cgcgcacgcg
      541 caattcgccg cgcgccgcgt cgtggtagta gcaggagccc ggcgcgcctg cttctgtctc
      601 gagggacagc tcggagagac cgtcggggaa gctgcgcaca ggtacgacca cctccgtcac
      661 cccgccgtcg ccgacccagc gtgcgacgcc tgtgccggtc gccacgtccc agtgaaagct
      721 cggcgacccg cccgcgaccg cgcgcagata cggccgcacg tacacgtcga ggatcggccg
      781 cggcacgccc gcggcgtcga ccagcgacag gtcctcctcg ttccacagcg cctcgttgcg
      841 cgagtactcc cacaacgtgc ccgacacccg ctcggcgtcg agggcgtcga ccacccgcgt
      901 caaccacgat gcgccagcgg tcccggcctc gtcggcgacg atgttgtacc cgaactcacc
      961 gatcaacggc gcgacgccct cggcgcgcga gaactcggcg aggctcgcca tgtgcacctc
     1021 cggctcgtga ccggcgtaca gctcgccgcc gaccagcccg ccgtcgtaca tgtgcggccc
     1081 gtacatcagg ttggtgccct ccggccgcat gtgcacggtc ggtgacaggc cggccgcctc
     1141 gacgccggta ttattgaaga ccagcagcag ctcgtcgccg accaccccgc gcatgtgcgc
     1201 gatcatctgc gtatgaaacg gcgtcagcgt gctcgccttc cacgcgttga tgttcggcgc
     1261 ggtgccccag cccggctcgt tgatcagctc gagcgcgagc acgccggggt gcgtcgccac
     1321 ccgcgacgcc atcttctccc acatcttgcg atacttgccc tggaccccgc cctcgtcggc
     1381 ccagaagcgg tcgaaggcct cgcgcacccc cgggtcgatg atgtacccga gcccccagtc
     1441 cgggcagtcg cgccgcggcg gcccgacctc gccggagatc gtccacagcg gaaagccgtc
     1501 gccgcaaaac ggcgacgcgt acacgtcctg atgaaagtcg acgatcaccc gcagccccag
     1561 cgcccacgcc gcgtcgacca tcgccacata acgatcgagg taacgctcgt cgtacatgcc
     1621 ctcgaccggc tcgagcgcct cccacgagaa cggcacgcgc accgtgtcga ggccccagcc
     1681 cggcagcggc gcgaagaatg ccgccgcgcc ggccttcacc tcgtcgaggc tcgcgtcctc
     1741 ggacatcggg aacggcatga acggggccca cttcgagcgc ccgcccgcgt tgaccccgcg
     1801 cagctggatg tcgcgcccga gcccgtcgcg taacaccccc atcgtgtcga cccgcagccg
     1861 cgcgtggctg cgctcgaccg gatcgatcgg ctcggcgcac gggccgtcgg tcaccgaccc
     1921 gccggtggtc ggcgtcccgc tcgtgctcgc cgaggtcccc gatgtcgtcg atgtctccgc
     1981 cgtctccgcc gtcgaccccg cgccgccgca gccgaccagc ccggtcagca accactcact
     2041 gcggatatgt ctgcgagatc gcgtagacgc cgaattgtgc gacccaatgt gcgtagttac
     2101 cataatcttc ggcccaatat tgcgggtgct cgaggtgcgt gatcatgtga tcgaggaaca
     2161 ggtcgcggta ggcgatgtcg tcggtcgctt cgtacagcga ccacaggccc cacgcgcgcg
     2221 agaaattgag cccgctgatg tgcgccttcg ccggcatgtc gatcggcgtc agcgccggaa
     2281 tcgccggcag ccagcccgcg agccacgtcg cctgcgcgtc cgcgggcagg aggttgacca
     2341 gcaggcgcgc gcggtgcaga catggcggga agaagtcgtc ggcgtcggcc tcctcctggc
     2401 tgaagggaca gctcgcgtcg aggcccggcg cctgcaaggt gttgagcgcg agcgactcca
     2461 tcgcgccggc gagctcgggg tcgttgtcga attcggccca ctgccacagg ttgagcaccg
     2521 cccacgtcag gctgttgtaa tcgtccgcga gcgcgccggc ctcgagctcc atcggcgtgc
     2581 gcgagtcgac ccactcgcgc agctgaccgc ggatctccat cgccagcggg cgcaggtcct
     2641 tcttgccggt cgacagctcg cgctcgcggg ccagcgtcag gaaccacgcg tagccgtacg
     2701 gcacctcctg cggcagcagg ccctgctgga tgcgcagcag ctcggccgcc aggccgacgt
     2761 ccgtcagctt cgcctccgcc gcctcgaggt agctcatgtc gccggtgtgg cggtacaccg
     2821 cgtgcaaggc gtaggtcgcg tgcaccgccg agtgccagtc gatgcaccca ttgaacaccg
     2881 ggtgctcggt gtcgttcatc gccacgcacg ccgagatcgg gaccgccagc gcgcgcagcc
     2941 agtcctcgcg cccgtcgagg aactcctgcc actcgtcgac cggcgcgccg gtcgtgtcca
     3001 tcatcgtgtc cgccccggtc gtcatgcccg ggccgggctc cccggtggtc ggcgtgtcgc
     3061 ccgtgctcga cgtgctggtc gagctcgtcg tgctcgaccc cgtcggctgc tcgccgccgg
     3121 tgctcgtcgc gccggccccg gtggtcgcgc tggcctcgcc ggtgctcgtt ccgccggtct
     3181 cgcccgcccc gccatcatca ccgcaggcga cgagggacag gccgaggatc atgacgaggt
     3241 cgcgggcgtt cacagttcca tgcaaagcac caagccacgc acctgtcaac cgcccggctc
     3301 ccggccattt cggcggcaat ggcagtatcg tcgccattgt tcatggtaag catgccggcc
     3361 gtccccgtcc cggtgacgct cccgtgtcga ccccgccctt gcatacgccg atcgcgctga
     3421 catgtcccgc gggacaggcc cctgcatgca acgcctgcgc cgcgcccgtc gacccagcgt
     3481 cctgtcctcg agggcaggcc cctgcatgca acgcctgcgc cgcgcccgtc gaccccgcgt
     3541 cctgtcctcg aggacagccg cgccgcgctc cgccctatcc tctcacctgg tcccggggac
     3601 atgcgcccgg ccgcggggtc gcgccatgaa gccggtcgcc accgcgccgc tcggccgtgg
     3661 tcgcctgttc gccacgatct tcgcgctcgc ggtcgcggcc gcgtgtgtcg gcgggctgct
     3721 cggctcgcgc gcgcgcggcc gcagcttcga gcaggcgcgc atgctgttcc accgcggcac
     3781 caccgggg
//
LOCUS       DASWNJ010001894         3329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_245221, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001894 DASWNJ010000000
VERSION     DASWNJ010001894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245221"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1937)
                     /locus_tag="VGB85_31545"
     CDS             complement(<1..1937)
                     /locus_tag="VGB85_31545"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0138676.1"
                     /translation="MKRCPACGTVYSVAQGFCPEDGAPLADDAPTGAPASASMSGGAV
                     VKGTWDPPRAGVSNSEMFVMPSQPRAVSASTGPGASTGNFVIPPLGSPGMTANGFMMP
                     PVSRPTTTGVAAAAVAISEPDPDPADTSTAFIPPITGAAKRTVVAMIPPGARSAGERL
                     ASLDAPRAGASGPTESAPGSGSSGASGLAQVPASAAVPGGADSGAFFPPGGLSRARPE
                     APAPSTWEESLIGRVLDQRYRIEGCIGAGGMGVVYKATHVIIDKPLAIKMLRADVASQ
                     PDVVKRFLLEAQLASKVKHPNVVDISDYGQIAGQTAYYVMEYLSGETLAHRVDTRGRL
                     DPALAVDIALQTAHALQAAHACKIIHRDLKAENIFLCDRPGGGVQAKILDFGIARIRD
                     KRTRLTAMGALIGTPAYMSPEQAQGSEVDERTDLYALGVILFEMLAGRVPFKAATVAL
                     VLSAQIFEAPPSLQDIDPGVPDLPNLEHVLQRLLAKNRDERPDDAGEVIHLLKTAAEI
                     DLGGHASGSAAATQTNKRRATVTIGSWSVAENPSGMHRSDNSPSVAGEARATPRPEAD
                     ALVVRPFTNSGRLQRRPSVIVPAGTHVERIAAPPVRPPITGEMEGRHYPTGVNPTIPQ
                     AGPPMILIIAAAASIGAAITVT"
     gene            2019..>3329
                     /gene="hutH"
                     /locus_tag="VGB85_31550"
     CDS             2019..>3329
                     /gene="hutH"
                     /locus_tag="VGB85_31550"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEY0138677.1"
                     /translation="MQPPAPIVIGRTLELADVHRVARHHVPAVLAPAARDELRRTRQH
                     LEAALARGEVIYGVNTGFGALSETRIAGDQLGALQQNLLRSHAVGVGPAFSGEIVRAL
                     LLLRAHTLALGASAASPELTEFLLALLTRDVRPVVPCQGSVGASGDLAPLAHLALAVI
                     GEGDAWFEGQVLPASEALARAGLRPRQLGPKEGLSLINGTQVMTAIAALAVVDAAELL
                     TAADILGALSLDALLGTIAASDPRIHAGKPHPGQQRSAEILRLLIADSPLNDSHRDCG
                     QVQDAYSLRCIPQVHGAVRGALAYVAGALRTEVNSFTDNPLVLARDDGGFDVISGGNF
                     HGATVAVPIDHMTAALTTLATISERRSDRMMNPQSSRGLPAFLADEPGLESGFMMAHV
                     TASALTSECKTLCFPASVDSIPTSANQEDHVSMSAHSARRLLPMV"
BASE COUNT          430 a         1229 c         1196 g          474 t
ORIGIN      
        1 gtcaccgtga tcgcggcgcc gatgctcgcg gccgcggcga tgatgaggat catcggcgga
       61 cccgcctgcg ggatcgtcgg gttgacgccg gtcgggtagt gacggccctc catctcgccg
      121 gtgatcggcg ggcgcacggg cggagcggcg atccgctcga cgtgggtgcc agccggcacg
      181 atgacgctcg gccggcgctg gagccggccc gagttggtga acggccggac caccagcgcg
      241 tcggcctcgg gccgcggggt cgcccgcgcc tcgccggcga ccgacgggct gttgtcggat
      301 cgatgcatgc ccgacgggtt ctcggcgacc gaccacgagc cgatcgtcac cgtcgcgcgg
      361 cgcttgttgg tctgggtcgc cgcggcggac ccggacgcgt ggccaccgag gtcgatctcg
      421 gcggcggtct tgagcaggtg aatgacctcg ccggcgtcgt cgggccgctc gtcgcggttc
      481 ttggcgagca ggcgctgcag cacatgctcg aggttcggca ggtcgggtac gccggggtcg
      541 atgtcttgca ggctcggcgg ggcctcgaag atctgggcgc tgagcaccag cgcgacggtc
      601 gcggccttga acggcacgcg cccggcgagc atctcgaaca ggatcacgcc gagcgcgtag
      661 aggtcggtgc gctcgtcgac ctcggagccc tgcgcctgct ccggggacat gtacgccggc
      721 gtgccgatca gcgcccccat cgcggtcagg cgggtccgct tgtcgcggat ccgggcgatg
      781 ccgaagtcga ggatcttggc ctgcaccccg ccgccggggc ggtcacacag gaagatgttc
      841 tccgccttga ggtcgcggtg gatgatcttg caggcgtgcg cggcctgcag cgcgtgcgcg
      901 gtctgcagcg cgatgtcgac ggcgagcgcg gggtcgaggc ggccgcgcgt gtcgacgcga
      961 tgcgccagcg tctcgccgct gaggtactcc atcacgtagt acgcggtctg tccggcgatc
     1021 tggccgtagt cgctgatgtc gacgacgttg gggtgcttga ccttcgaggc cagctgcgcc
     1081 tcgagcagga agcgcttgac gacgtccggc tggctggcga cgtcggcgcg cagcatcttg
     1141 atcgccagcg gcttgtcgat gatgacgtgc gtcgccttgt aaacgacgcc catgccgccg
     1201 gcgccgatgc agccctcgat gcggtagcgc tggtcgagca cgcggccgat cagcgactcc
     1261 tcccaggtgc tcggggccgg cgcctccggg cgcgcgcgcg acagaccccc gggcgggaag
     1321 aaggcgccgc tgtccgcgcc gcccggcacc gcggccgacg ccggcacctg cgccagcccg
     1381 ctcgcgcccg aggatccgct gccgggcgcc gactcggtcg gacccgacgc gcccgcgcgc
     1441 ggcgcgtcga gcgaggcgag ccgctccccc gccgagcgcg cgcccggggg gatcatcgcc
     1501 acgacggtgc gcttggccgc gccggtgatc ggcgggatga aggcggtgct ggtgtcggcg
     1561 gggtcagggt cgggctcgct gatcgcgacg gccgcggccg ccacccccgt ggtggtgggc
     1621 cgcgagaccg gcggcatcat gaacccgttg gcggtcatgc ccgggctgcc gagcggcggg
     1681 atcacgaagt taccggtgct cgcgcccggt ccggtcgagg ccgacaccgc tcgcggctgc
     1741 gacggcatca cgaacatctc cgagttggac acgcccgcgc gaggaggatc ccacgtcccc
     1801 ttcacgacgg cgccgcccga catgctcgcg gacgccggcg ctcccgtcgg cgcgtcgtcg
     1861 gcgaggggag ccccgtcctc cgggcagaag ccctgggcca cggagtacac cgtgccgcac
     1921 gctgggcagc gcttcatgac tgcgccagcg tagacggtgg gcactgcggg gtcaacgctt
     1981 gcgcagggtg gccggcgccg gtgtaccctc cacgcccgat gcagccccct gcccccatcg
     2041 tgatcggtcg cacgctcgaa ttggccgacg tgcaccgggt cgcccggcat cacgtacccg
     2101 ccgtgctggc tcctgcggcg cgcgacgagc ttcgccggac ccggcaacac ctcgaggctg
     2161 cgctcgctcg cggcgaggtc atctacggcg tcaacaccgg cttcggtgcc ctcagcgaga
     2221 ctcgcatcgc cggggaccag ctcggtgccc tccaacaaaa cctgctgcgc agccacgcag
     2281 tgggcgtcgg acccgcgttc tccggggaga tcgtgcgagc gctgctactt ttgcgcgccc
     2341 acaccctcgc gctcggggcc tcggccgcgt cccccgagct gacggagttc ctgctcgccc
     2401 tgctgacccg tgacgtgcgt ccagtcgtgc cgtgtcaggg cagcgtcggc gccagcggtg
     2461 atctcgcgcc gctcgcccac ctcgcgctcg cggtgatcgg cgagggcgac gcctggttcg
     2521 aaggacaggt ccttccggcc agcgaggcgc tcgcgcgggc cggcctgcga ccgcgccagc
     2581 tcggccccaa ggagggcctg agcctgatca acggcacgca agtgatgacc gcgatcgcgg
     2641 cgctcgccgt cgtcgacgcg gccgagctgc tgaccgcggc cgatatcctc ggcgcgctga
     2701 gcctcgacgc cctgctcggc acgatcgccg cctccgatcc gcggatccac gcgggtaaac
     2761 cccacccggg ccagcagcgc agcgccgaga tcttgcgcct gctgatcgcc gactcgccgc
     2821 tcaacgactc gcaccgcgac tgcggccagg tccaggacgc ctactcgctg cgctgcatcc
     2881 cgcaggtcca cggggccgtc cgcggcgcgc tcgcctacgt cgccggggcc ctgcgcacgg
     2941 aggtcaacag cttcaccgac aacccgctgg tgctcgcgcg cgacgacggc ggcttcgacg
     3001 tcatcagcgg cggcaacttc cacggcgcga ccgtggcggt cccgatcgat cacatgaccg
     3061 ccgcgctgac gaccctcgcc acgatctccg agcgccgcag cgaccgcatg atgaaccccc
     3121 agagctcgcg cggcctgccg gccttcctcg ccgacgagcc cggcctcgag agcggcttca
     3181 tgatggccca cgtcaccgcc tcggcgctga cgagcgagtg caagaccctg tgcttccccg
     3241 ccagcgtcga ctcgatcccg acctcggcca accaggagga ccacgtgtcg atgtcggcgc
     3301 acagtgcgcg ccgcctgctg ccgatggtc
//
LOCUS       DASWNJ010001895         1933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_104879, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001895 DASWNJ010000000
VERSION     DASWNJ010001895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104879"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1194)
                     /locus_tag="VGB85_31555"
     CDS             complement(94..1194)
                     /locus_tag="VGB85_31555"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY0138678.1"
                     /translation="MDSQDFRLEQFQFEDLRDDELLVEPLFGSWEGNMSHSVARRPID
                     ICRRRREPRVVIGNASVLRVLRAGSAVRGVAEGAVGILYSGYGIDEFGYTVSAHGYDA
                     PGTIGMLAKRSKIPARNFLPLPVGSTVALERWAAFAVRYLTAWSNWKLAFGAYRLQMS
                     AADDPAPFVLGWGGGSTYAELDLARRHGCRVAMISGSEERLAWLAQRDIHGIDRRAFP
                     DLETDESRMADPAFKRAQQASERAFLATVKAWTGGAGAAVMVDYIGGPVFRASLRALG
                     RQGVLTTAGWLLGMQTPINRAIECIARHIHVHTHFIRHGDCAEAMEYAVANEWLPEVS
                     EIYDWDEVPRLARNAAAGQLQTYFPVYRVNPV"
BASE COUNT          342 a          702 c          605 g          284 t
ORIGIN      
        1 cgccgcggcg tcccgccgac tctgatctgc tctcatggct aatcctcgct caccggcggc
       61 cggtcagatc gcacacatcg cggacacgcc ggctcagacc gggttcacgc ggtacaccgg
      121 aaagtaggtc tggagctgcc ccgcggcggc gttgcgcgcc agcctgggca cctcgtccca
      181 atcgtagatc tcgctcacct ccggcaacca ttcgttcgcc accgcgtact ccatcgcctc
      241 cgcgcaatcg ccgtggcgga taaagtgggt gtgaacgtgg atgtgccgcg cgatgcactc
      301 gatcgcgcga ttgatcggtg tctgcatccc gagcagccag cccgcggtgg tcaggacacc
      361 ctggcggccg agcgcccgca ggctcgcgcg aaacaccgga ccaccgatat aatcgaccat
      421 caccgccgca cccgcgccgc cggtccacgc cttcacggtg gcgaggaacg cccgctccga
      481 ggcctgctgc gcgcgcttga acgccgggtc ggccatgcgc gactcgtcgg tctccaggtc
      541 cgggaaggcg cggcggtcga tgccgtgaat gtcgcgctgc gccagccagg cgagcctctc
      601 ctcgctgccg gagatcatcg ccacgcggca gccgtgtcgg cgcgccaggt cgagctcggc
      661 gtaggtcgag ccaccgcccc agccgagcac gaacggcgcg gggtcgtcgg ccgcgctcat
      721 ctgcagccga tacgccccga acgcgagctt ccagttcgac caagcggtca aatagcggac
      781 cgcgaacgcc gcccagcgct cgagcgcgac cgtcgagccg accggcagcg ggagaaagtt
      841 gcgcgcgggg atcttcgagc gcttcgccag catcccgatc gtgcccggcg cgtcgtagcc
      901 gtgggccgac accgtgtatc cgaactcgtc gatcccgtac ccgctgtaga ggatcccgac
      961 cgcgccctcc gcgaccccgc ggaccgccga accggcccgc agtaccctca ggacgctcgc
     1021 gttgccgatc accacccgtg gctcgcgacg gcgacggcag atatcgatcg gtcgccgcgc
     1081 gaccgagtgc gacatattcc cctcccagct gccgaacagc ggctcgacga gcagctcgtc
     1141 gtcccgcaga tcctcgaact ggaactgctc gagccggaaa tcctgcgaat ccaacgcaga
     1201 tccagcgcct tcgggcccct cgtggaggac ccaagcttgt gcattgatca cgattacctc
     1261 cctcgcgcga gctcgctcgc gccccaccgc ttcagacccc gaaaatgccc cgcgactgtg
     1321 acatggatcg catctgcatg gaagacgggt cctccgattt ttgccaaaaa aatgcgcaca
     1381 ccccgggtcg ctattcgagc gacccgtgca atagagacga agattcgggg cctcccgccc
     1441 tcggtgccgc gaccgctcgt cgacgaggaa tcggcgggtg agtatcgcgg ctgcgacacg
     1501 acgacccaaa cgcgaatcga cgatcgccgc gccacaccgt cgccgcgcag ggcgcgatcc
     1561 agggggacac actcgtgtcg cccgccggcc ccactgtcag gcgcttctgg caccatcgat
     1621 cggtatggat ggcactcagc ccgaggaccc caccgcccga cccggatact ggtctcgcac
     1681 tcgcggcgat tccacgagcg tcatcaccag gcagggcacg gtccgctggt ggcagatctt
     1741 cgcgaacaca atgcccgacc cggtaccccg tgccccctcg gaattgtttg gaacgcggcc
     1801 ggtcggcgga aaggaggggc aacgggagag gtggattgtc gaatcaacgt tgatcaacgt
     1861 cgacggtggt atcaagctcc cggccaggag gagcgacgat ggatacgacg acgatgacga
     1921 cgagcgggct cga
//
LOCUS       DASWNJ010001896         2565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_527598, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001896 DASWNJ010000000
VERSION     DASWNJ010001896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527598"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VGB85_31560"
     CDS             <1..261
                     /locus_tag="VGB85_31560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138679.1"
                     /translation="EPLQVAINVDEFGQTNNTHVWTASTADGQPYPGVDFCGDWTGVK
                     GSASYGWSGSTDAEWIEISAEVAGQPSTDCASEAALYCVEQQ"
     gene            258..1436
                     /locus_tag="VGB85_31565"
     CDS             258..1436
                     /locus_tag="VGB85_31565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138680.1"
                     /translation="MTMTLGHRFPPLALLLAGCPAYPVLPDAAESGSSSSSTGDLDPP
                     GGVPTGTDTDDVPGPGTTGEASNGGSTGSVAVCGDGIVDSGEGCDDGAGNGPNAACTS
                     ACRPNVCGDGDVYAGVEGCDEGAENVDTGYCRSDCQLGVCGDGFVFAALEECDAAEAN
                     GTVYGQCDTNCTINRCGDGELDAGFEECDEGELNGSGQDDEMGLAGCDLDCGFAGRRI
                     FLSSQFFTGDMGTRAGADLACETMAYAAGLRHSERFKALLADAKGAPNDFVQANPQDD
                     RPFILPTGVVLAASYPDLIASGPGAGITTTELGEVLYEKKVWTNVNPFGDAYLEDPAS
                     TCASWTSADKLNSARLGYNAVAPGDAAALAEWTAKKQWLSFSSEGCNFSYRIYCVEAS
                     "
     gene            complement(1497..2225)
                     /locus_tag="VGB85_31570"
     CDS             complement(1497..2225)
                     /locus_tag="VGB85_31570"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY0138681.1"
                     /translation="MEPAQIDLFREQGWLRLAGFYSCRRMAPIKQVVLDALKRSKIWA
                     TGKSLSRRLQDLPVFQQNGQLASLVAVPGLHDACITRELIAVMTELAGRLPTSSQAAR
                     LLLSLPHQGTWTLQGLNWHVDMAVKPGELAHGVQSFILIDDVAPRGGATLALAGSHRA
                     VLRGGTTASRLREALKTSPDLAASLRDMGLAVIEMSGRAGDIFLMDMRLLHTPSINAT
                     KNLRMMATARYFFDHPTSLPGPRT"
     gene            complement(2277..>2565)
                     /locus_tag="VGB85_31575"
     CDS             complement(2277..>2565)
                     /locus_tag="VGB85_31575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138682.1"
                     /translation="RRRLVGGKGVGEQLVPGPGDIDNGVTDADSRREIGLVSLDTSAG
                     QPALRLLTRNGLEDHTPMFTADSQTVVFRTRFPFEKTNWTMTTARSVPARK"
BASE COUNT          420 a          829 c          922 g          394 t
ORIGIN      
        1 gagccgctgc aggtggcgat caacgtcgat gagttcgggc agacgaacaa cacccacgtg
       61 tggaccgcct cgaccgcgga cggtcagccc tatcccgggg tcgatttctg tggcgattgg
      121 accggcgtca agggctccgc cagctatggc tggagcggga gcaccgacgc ggagtggatc
      181 gagatctccg ccgaggtcgc cgggcagccg tcgacagact gcgcgtccga ggccgccctg
      241 tactgcgtgg agcaacaatg accatgaccc tcggacatcg atttccccct ctcgcattgc
      301 tgctcgcggg ctgtcccgcg tatcccgtgc tgcccgacgc cgcggagtcg gggtcaagct
      361 cctcgtcgac cggcgacctc gacccgccgg gcggggtccc gaccggcacg gacacggacg
      421 acgtgccggg cccggggacg accggggaag cgagcaacgg gggtagcacc gggagcgtcg
      481 cggtctgcgg ggacggtatc gtcgactcgg gcgagggctg cgacgatggc gcaggcaacg
      541 gtcccaacgc ggcgtgcacg agcgcgtgcc ggcccaacgt ctgcggcgac ggcgacgtgt
      601 atgccggcgt cgagggctgc gacgagggcg ccgagaacgt cgacaccggc tactgccgat
      661 cggactgcca actcggggtg tgtggggacg gatttgtgtt cgccgcgctc gaggagtgcg
      721 acgcggccga agccaatggg accgtatatg ggcagtgcga taccaactgt acgataaacc
      781 gctgcggtga cggcgagctc gacgccggct tcgaggagtg cgacgagggc gagctcaacg
      841 gttcgggcca ggatgacgag atgggcttgg ccggctgcga cctcgactgc ggcttcgcgg
      901 gccgcaggat cttcctgtcg tcacaattct ttacgggaga catgggcacc cgcgccggcg
      961 ccgatctggc ctgcgagacg atggcctacg cggcgggact gcggcacagc gagcgcttca
     1021 aggctctgct cgccgacgcc aagggggcgc cgaacgactt cgtgcaggcc aacccgcagg
     1081 acgaccggcc cttcatcctc cccaccggcg tcgtgctcgc cgccagctac cccgatctca
     1141 tcgcgtcggg gcccggcgct ggcatcacga ccaccgagct cggcgaggtc ctgtacgaaa
     1201 agaaggtctg gaccaacgtc aatccgttcg gcgacgctta cctcgaggac cccgcctcga
     1261 cctgcgcgag ctggacctcc gccgacaagc tcaactcggc ccggctcggc tacaacgcgg
     1321 tcgcgcccgg ggacgcggcg gcgctcgccg agtggacggc caagaagcag tggctcagct
     1381 tttcgagcga gggctgcaac ttttcatatc gcatctactg cgtcgaggcc tcgtagctgt
     1441 ttccccatcc gctcatgtgc gagcgggcac cggccatgcg gtggtcatcg tccaactcat
     1501 gttcgcggtc cggggagcga ggttgggtgg tcgaagaaat agcgggccgt cgccatcatg
     1561 cgaaggttct tcgtcgcgtt gatcgatggg gtgtgcaaca ggcgcatgtc catgagaaag
     1621 atgtcgcccg cccgacccga catctcgatg acggcgaggc ccatgtcgcg gaggctggcc
     1681 gcgaggtcgg gggaggtctt gagcgcctca cgaagcctgg aggcggtcgt gcccccacgc
     1741 aggacggcgc ggtgagagcc cgccagcgcc agggtcgcgc ccccgcgcgg cgccacgtcg
     1801 tcgatcagga tgaaggattg aacgccgtgg gcgagctctc ccggcttcac ggccatatcg
     1861 acgtgccagt tcaatccctg cagagtccag gtgccttgat ggggcagcga cagcagcagt
     1921 cgagcggctt gcgaggatgt gggcaaccgc cccgccagct cggtcatgac ggcgatcagc
     1981 tcgcgggtga tgcacgcgtc gtgcaagccc gggaccgcga cgagcgacgc gagctgtccg
     2041 ttctgctgga agacgggcag gtcttgcagc cgcctggaca ggctcttgcc ggtagcccag
     2101 atcttcgatc gcttcagcgc gtcgagcacc acctgcttga tcggcgccat ccgtcgacag
     2161 gaataaaaac ccgcgaggcg cagccagcct tgctcacgga agaggtcgat ctgggccggt
     2221 tccatgggcc agtcgcgagc gcccggccgg ggtgagccgg ccggggcgag ggacgctcac
     2281 ttgcgagcgg gcaccgagcg ggcggtggtc atcgtccagt tggtcttctc gaatgggaag
     2341 cgggtgcgga acacgaccgt ctggctgtcg gcggtgaaca tgggggtgtg gtcctcgagg
     2401 ccgttgcggg tcagcaggcg cagggcgggc tggccggcgg aggtgtcgag ggagaccagg
     2461 ccgatctcgc ggcggctgtc ggcatcggtg acgccgttgt cgatgtcgcc aggccctgga
     2521 accagctgtt caccgacacc cttgccgccg acaagacgcc gccgc
//
LOCUS       DASWNJ010001897        14911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_33378, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001897 DASWNJ010000000
VERSION     DASWNJ010001897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 14911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14911
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33378"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..554
                     /locus_tag="VGB85_31580"
     CDS             261..554
                     /locus_tag="VGB85_31580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138683.1"
                     /translation="MKHITRFTLLATLAVLLPTGCARTSADKAGSETPTSANAGPENT
                     DPGLEPLGDPVDQVGMKEYLPPTDGAAPNKPGTEDTGNPGGSTVNEVGSNPKK"
     gene            complement(612..2000)
                     /locus_tag="VGB85_31585"
     CDS             complement(612..2000)
                     /locus_tag="VGB85_31585"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0138684.1"
                     /translation="MTHDDLGLQLSEVSTATSTATAATRAMNVEIPREGERVKVKTLP
                     GTVTGEVAGTALRDPVALARALLGDSGGRPRETHTLRVLAGALVSLGFTVRELSVGEG
                     VNRRRTLFARIGEGSPRVGFAAHVGATEKADMKGAVAGFIAAVARMQETRQIRPQGSL
                     SLLLTTEAADATTPGIAAVTDWLTTQGELPEVCIVGAPTSRARLGDVIKIGCRGTLHG
                     VVTVQGRAGHTACPELADNPVSRLLRFLSRLDEAQLDHGSSHFPPSSLTVTTVDVGNP
                     ASHVIPDTASAAFEIRYNDLHTRLSLESWLRRLAVAQLGSAHQLELRGTGDTTVKPPG
                     ALSEAMCAAISAITGQRPAIDTGSEDAGVRLIRRHCPVVEFGPRGETMHEADEQDALT
                     ELEQLTQVYQGVMSRLLADDSRARSRGNNSRHDATQGTPGRITPSAVFSSISMCLQAA
                     TAYAQDGAAVVA"
     gene            2225..3901
                     /locus_tag="VGB85_31590"
     CDS             2225..3901
                     /locus_tag="VGB85_31590"
                     /EC_number="3.1.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF027980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ca2+-dependent phosphoinositide-specific
                     phospholipase C"
                     /protein_id="HEY0138685.1"
                     /translation="MQRLSRLALLLAACHRPADAIQSTAAASTGDTTIPPDTSASTSN
                     GTALPTSDATEATATSTTSTTGSTTEDIGTGDTGTTGDPVGLRYDAVRQKSAHNSFQR
                     DETLLDQIVFHRVRSLELDIHHSSSKSPTIAGEWYVYHIDIADDASHCRRLSQCLAQI
                     AAAAQALPEHEVITLWIDLKDPFVAEHQPADLDALLTKAFGERLLTPADLRAACPAAT
                     NLREAVTFPGCSWPELAASHGRVIVTLTGGGLADPASKLSTYVGADASARAAFAAPDL
                     DDPADLDASPAIFHNLALADAGLATAVRSAGLVSRVWVADDADAWATAEAAGVHHIAS
                     NKVNAAIDPWATTASPGGYPFTCIDGCTTPDTEAGAVLRATVDSGDLWADSDDALLAT
                     WTPDGDVTLTALLAVPSSHIEPFAKACLIARESAAPGAPYLAVCRPTDEQPLRVQLRE
                     TAGADSEAIELADIDGLSSETPAFARLERTGACVRGLTSIDGMQWTELGAHCFPAPLP
                     LVGLTASSHDAGEVRVLFVAPTASPGGALAAGTLTVTPVGQAKGSVADGL"
     gene            complement(3868..4509)
                     /locus_tag="VGB85_31595"
     CDS             complement(3868..4509)
                     /locus_tag="VGB85_31595"
                     /inference="COORDINATES: protein motif:HMM:NF016879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Coq4 family protein"
                     /protein_id="HEY0138686.1"
                     /translation="MIAWRQVRRAIAMFRAGRYGDAAVLKADAAGTRALPEIEAQLGP
                     VRGYMPAVDVDYLRNLPPGSFGRAYVDFLDHNGISPLVISPELAEEVQRNTFAVRYVA
                     THDMFHTVLGFDTSLPGEAGVYAFAAEQRYSGSSELLLRSARIVYTLRKPWQWRQIRD
                     AIRRGRALARRARMLLAFRFEEHWERPLVELRELLGIDARGAYHSPSATLPLA"
     gene            complement(4511..5179)
                     /locus_tag="VGB85_31600"
     CDS             complement(4511..5179)
                     /locus_tag="VGB85_31600"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0138687.1"
                     /translation="MPTQTFLNLAADKRERIVELALEEFSQRPYAQASLSRIVARAGI
                     AKGSIYQYFTDKFDLYRWLVVEEVGRRKQAFIGDALPVEGDLRTRLRAMCIAGLRFAL
                     THPRLARVAAHAAAPGDEPALQALHAEMRAIGRAGLEALLRGAQAAGELRADLALAPA
                     AAVLTAALGDGMMNALLERLGLTLPEYLADTGLASRLSPEETAELIDSLLDVLWNGIA
                     DRRM"
     gene            5555..6118
                     /locus_tag="VGB85_31605"
     CDS             5555..6118
                     /locus_tag="VGB85_31605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138688.1"
                     /translation="MAWCSIDRVGVGACRLAVLASLAGAGCRTWEDVAVTEMLSVPTA
                     ADAPCDALARDLAAPGGSLAIAAIEFSDTNVLALDETLVIRARVPHYSEYTRKLDREE
                     QVDGFDLELQCPQWGDPTPLTVRMCSEDGSAFGIVDAAQLRRLTDHGALGDTCRLWGR
                     LWTGDRTRPWMASVIVRPSLRSLVEVQ"
     gene            6118..6702
                     /locus_tag="VGB85_31610"
     CDS             6118..6702
                     /locus_tag="VGB85_31610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138689.1"
                     /translation="MRAAVAAALLTGCAHTSAPRPTPVSAHFMAASAVEDCVEMARRL
                     DRIGAWAPRYRNERGEEVALYPSDELWVLNMAMVPPARDGSAAQGLRSLTLTCGAEQR
                     ALQAVACRNGTGLHLYVDPAQLTGWQTRGTVDAGCRVDADVAGIVYRDERKILLRAGL
                     RERLGPIDPSPNRYNEPPARTTRVARPVAAESLE"
     gene            complement(6717..7352)
                     /locus_tag="VGB85_31615"
     CDS             complement(6717..7352)
                     /locus_tag="VGB85_31615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138690.1"
                     /translation="MDASAVVGCLVSSALMMGDPSGPPVTAEAPAPVDPFAVPVTPPQ
                     VIGVDGYLAGQHERAKRLHRRVDFDFRRHWADYEGEQRENAAEGPEDQDEPETFYEYM
                     ARKAQRKRIGGAVMLASGGLAIVAGGLLFWAADDELILIATVPMWAGGLVLGIVGGVK
                     LGVGSRMSRDLYDEAKKLKISRTRPRLELRGIAPLYSPRSQATGISLGFAF"
     gene            complement(7530..7928)
                     /locus_tag="VGB85_31620"
     CDS             complement(7530..7928)
                     /locus_tag="VGB85_31620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138691.1"
                     /translation="MSELLIGPHRLRVEGDTVLTRYIGVPELEHVQEIHRHFERVFAE
                     HGRLFIINDMHHTGVPATATRKWISEWARAHPITGHVSFGASLPIRLLQALVMRASAL
                     FSKQPLTAAEHVDGEAEAFAWVAARRQQPR"
     gene            8088..9935
                     /locus_tag="VGB85_31625"
     CDS             8088..9935
                     /locus_tag="VGB85_31625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138692.1"
                     /translation="MPARPTSPRRCPRISALAALLVLCACNGAGSTDTGASTDASSGN
                     TPTTGADPDPTSTGGEPGPTTSTTSTTSTTAATSTTSTSDPTTTSGSDTTGATTGEDT
                     GAAQCPPPLEQPPGSWTSAIVPVGDDDALTYVADADGNRIPDFSYAGYHRGEAAIPDV
                     PTALELGPIDGDDTEQIQDAIDEVGAMAPGPDGLRGAVVLAPGEYQIAGTIELRHSGV
                     VLRGAGDGGDPTSDTILQAVGDTPHQRVVIVVGSGDNNRWEPELPGTRTDIVSELVQI
                     GDRQLEVEDPGAFAVGDHVVIVHPCTDAWLTAVDHGGTADDGPWTVDSQPIVFKRQLL
                     AIDGATLTLDAPVFNHLDRSLAQSYIYAQDRKGLVTEVGVEDLRVDIETAGGEDEDHA
                     WSAIAMRGVEDGWVRRFTALHFGFAAVTVQTGTQITVSDSRGIDPVGIITGERMYNFD
                     ASGGQQVLFTGCHASGGRHHYVSNGTTWTSGVVFHRSTSTDAHASSEGHRRWSMGMLY
                     DNVVESAPSKDGMVVLGLYNRGDYGTGHGWASAHSVAWNYDTSTGVGAIQRPPTAQNY
                     AIGGAGKFTGNKPAPFAQPEGYIEGTGKPGLVPESLYDRQLAERLCGLR"
     gene            10038..11600
                     /locus_tag="VGB85_31630"
     CDS             10038..11600
                     /locus_tag="VGB85_31630"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0138693.1"
                     /translation="MDPRAVLEPTLRSPPAWDPAATVAGGGADETIAGAPETAAEPLE
                     ARFIGHFAVLRKLGEGGMGVVYAAYDERLERRVALKLIHGDDPVRLMREAQALARVSH
                     PNVVQIYEVGAYADRVFVAMEYVEGPSLAEWLAEPRDIGAVLEVFVQAGRGLEAAHAR
                     GLVHRDFKPGNVIVGADGRARVLDFGLARADDRREEAVPADISAARTTDANLLSSPLT
                     RTGSLLGTPAYMSPEQFLGVPVDARSDQFSFCVALYEALYDRRPFAGATMPELMAAVT
                     HGEVELPPPRPELAETIPAAVLRGLVAEPDRRWPTMTELVDVLDAARGQVDERSRAQA
                     GGSIAWIITIAGLAMFLAVNVLWDPGDVTPASMTRFAVMIALVAVAALPTLRARYREP
                     QQRVYVNFGVAMPAMSMVARFFSWRAGLSMSDMAVTESLCFVTAYMTMAVFLRAPLVL
                     INIPVLLGALVLVHELGAPGQILALSWLLSIPVTAAAWWYTALRPRRRGATSQTSMSA
                     GPVMPSINTLTR"
     gene            12047..12175
                     /locus_tag="VGB85_31635"
     CDS             12047..12175
                     /locus_tag="VGB85_31635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138694.1"
                     /translation="MSKLLRGAVAGAGAWKLGGGFIGTILLFILLWVVLGNFDIFK"
     gene            12967..14688
                     /locus_tag="VGB85_31640"
     CDS             12967..14688
                     /locus_tag="VGB85_31640"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138695.1"
                     /translation="MKNYDHTTPHRRANFAFAVYAGIQVACSADSPVTSQPTHPSATG
                     TTGATSSGGLTGATEADTSMIPTTSMTPAESSNTTMQTSDGSTSWSGTSDATTASSGT
                     SDATAASESSTGAAHDWSLEVEDISPGLGIHIDGPMQLVGIGDHFAYVDGWGQDGVSD
                     LVINARFGLVDGNYSSSFDLADVEKYATAVLITVVGGFRGAYASVGDINGDTIEDLII
                     GEPGYYIEGYDEDYLGEGRCHLVWGPAELEDIDVPSLFEQGLGSRVTGVDYGQEVGRR
                     VASAGDANGDGLSDTVMTTVGNSRSALVFGDASPMSLSTLDIEAGMGGYPIEAPASSL
                     LLPLGDVDGDGLADHTQIGSSQMGSTISIIYGKNDVEPVTLDGLKVGEGGFIIDVSTL
                     YSSGADFGVSGASLGDFDGDGWHDLVVTAGVAPAARRIFMIRGAESWGQPTLATLQAE
                     DRIRLFEGPDYPGGVLSFVDDLNGDGRDELLAGWPFSNTVYLVFGQEGTHTISLESLV
                     EDKTGVKFRNTVGHIGLGRGLIGTRDLTGDGIAELVLGAPQASPMGRPGAGQVFIVDG
                     AALVAWL"
BASE COUNT         2331 a         5306 c         5009 g         2265 t
ORIGIN      
        1 acgagcggcg agctgaagtt cgagctgcgc cgcgacgcct cggccatgag cgacacatat
       61 ggctgacacg acttcacgat cggcgccgtc gtcgtcatga tcagcaatgg aggtttcgtg
      121 accccggtgg cacctgcgca cacatgccgt gccggtcatc gcgatcggct tcgcggctgc
      181 cacgcgtgag ccgaacgcgg cgagctggcg cgcccggccg gcccgtggcg ccggccgccg
      241 atcacggcca cggatcgatc atgaagcaca tcacgcgatt caccctcctc gccacgctcg
      301 ccgtgctgct gcccaccggt tgcgcccgca cctccgccga caaggccggc agcgagactc
      361 cgaccagcgc caacgccgga cccgagaaca ccgaccccgg cctcgagccc ctcggtgacc
      421 cggtcgacca ggtcggcatg aaggagtacc tgcccccgac cgacggcgcc gccccgaaca
      481 agcccggcac cgaggacacc ggcaaccccg gcggctcgac cgtcaacgag gtcggcagca
      541 acccgaagaa gtagaaaacg cgcgagcgcg tcatcgccga gcgatgacgc gctccgtgac
      601 tccgtgttcc ctcaggcgac gaccgcggcg ccgtcctgcg cgtacgcggt cgcggcttgc
      661 aggcacatgc tgatgctcga gaacaccgcc gacggcgtga tgcgcccggg ggtgccctgc
      721 gtggcgtcgt ggcggctgtt gttgccccgc gagcgcgcgc ggctgtcgtc ggcgagcagg
      781 cgggacatca ccccctggta cacctgcgtg agctgctcga gctcggtgag cgcgtcctgc
      841 tcgtcggcct cgtgcatcgt ctcgccgcgg ggtccgaact cgacgaccgg gcagtggcgg
      901 cggatcagtc gcacgccggc gtcctcgctg ccggtgtcga tcgccggccg ctggcccgtg
      961 atcgcggaga tcgccgcgca catcgcctcg ctgagcgcac ccggcggctt gaccgtcgtg
     1021 tcgccggtgc ccctgagctc gagctgatgc gccgaaccga gctgcgcgac cgccaggcgg
     1081 cgcagccacg actcgaggct gaggcgcgtg tgcaggtcgt tgtagcggat ctcgaacgcg
     1141 gccgaggcgg tgtccgggat cacgtgtgac gccgggttgc cgacgtcgac cgtggtgacc
     1201 gtcagcgacg acggtgggaa gtgcgatgag ccatgatcga gctgcgcctc gtcgagccgc
     1261 gacaggaacc gcagcagtcg cgacaccggg ttgtccgcca gctcggggca cgcggtgtgc
     1321 ccggcccgac cctgcaccgt cacgaccccg tgcagcgtcc cgcgacaacc gatcttgatg
     1381 acatcaccga gccgcgcgcg cgaggtcgga gcgccgacga tgcacacctc cggcagctcg
     1441 ccctgcgtcg tcaaccagtc ggtgaccgcg gcgatgcccg gcgtcgtcgc gtcggcggcc
     1501 tccgtcgtca gcagcagact caacgacccc tgaggccgga tctgccgcgt ctcctgcatc
     1561 cgcgcgaccg cggcgatgaa gcccgccacc gcgcccttca tgtccgcctt ctccgtcgcg
     1621 ccgacgtgcg ccgcgaagcc gacccgcggg ctgccctcgc cgatccgtgc gaacagcgtg
     1681 cgtcgacggt tcacaccctc gccgaccgac agctcgcgca ccgtgaagcc gaggctgacc
     1741 agcgcacccg ccagcacgcg cagcgtatgc gtctcgcgcg gccgcccgcc cgagtcgccg
     1801 agcagcgccc gcgcgagcgc caccgggtcg cgcaaggcgg tcccggcgac ctcgcccgtg
     1861 accgtgcccg gcagcgtctt gaccttgacc cgctcgccct ccctgggaat ttccacgttc
     1921 atcgcgcgcg tcgcggcagt cgcggtggag gtcgcagtgg agacttcgga aagttggagg
     1981 ccgaggtcgt cgtgtgtcat gggcactcca tcctgcacga agctgcgcac ctcgagcgcc
     2041 aactcgtcgg attactgcga agtcacgtgc tcacgactgc acgcctgcgc catcgcgaca
     2101 aacctcgcag gctgcgcacg gtcatatcac cgggcgctcg ttccgctgtg ctcgcttacc
     2161 tcgcgggatt ataccagtga ggaccattgc ccccgcggcg gttcgacgcg catgctcggc
     2221 gtcgatgcaa cgtctgtcgc gcctcgccct gctgctcgct gcctgtcatc gccccgccga
     2281 tgcgatccag agcacagctg cggcaagcac cggcgatacg accattccgc ccgacaccag
     2341 tgccagtacc agtaatggca ctgccctgcc gacgagcgac gcgacagagg cgacggccac
     2401 cagcacgacc agcacgaccg gcagcacgac ggaggacatc ggcactggcg acaccggaac
     2461 gaccggcgac ccggtgggcc tgcgctacga cgccgtgcgc cagaagtcgg cgcacaacag
     2521 cttccagcgc gacgagaccc tgctcgacca gatcgtgttt caccgcgtcc gctcgctcga
     2581 gctcgacatt catcacagca gctcgaagtc gccgacgatc gccggcgaat ggtacgtgta
     2641 tcacatagac atcgcggacg acgccagcca ctgtcgccgc ctctcgcagt gcctcgcgca
     2701 gatcgcagcc gccgcgcagg ccctgcccga gcacgaggtg atcaccctgt ggatcgacct
     2761 caaggacccc ttcgtcgccg agcatcagcc cgccgacctc gacgcgctgt tgacgaaagc
     2821 gttcggcgag cgcctgctga cccccgccga tctgcgggcc gcctgcccgg ccgcgacgaa
     2881 cctgcgcgag gccgtcacct tccccggctg cagctggccc gagctcgccg cgtcgcacgg
     2941 ccgtgtgatc gtcacgctga ccggcggcgg cctcgcggac cccgcgagca aactgtcgac
     3001 atatgtcggc gccgacgcca gcgcgcgcgc agccttcgcc gccccggacc tcgacgaccc
     3061 cgccgacctc gacgcctcgc ccgcgatctt tcacaacctg gcgctcgccg acgccggcct
     3121 cgccaccgcg gtgcgctcgg ccggcctggt cagccgcgtg tgggtcgccg acgacgccga
     3181 cgcctgggcc accgccgagg ccgcgggcgt gcaccacatc gccagcaaca aggtcaacgc
     3241 ggcgatcgac ccatgggcga ccaccgcgtc gccgggcgga tatccgttca catgcatcga
     3301 cgggtgcacc acgcccgaca ccgaggccgg cgccgtgctg cgcgcgaccg tcgactcggg
     3361 tgacctttgg gccgactccg acgacgccct gctcgcgacc tggacccccg acggtgacgt
     3421 gacgctcacc gcattgctcg cggtgccgag cagccacatc gagccgttcg ccaaggcgtg
     3481 cctgatcgcg cgcgagagcg cggcccccgg cgcgccctac ctcgcggtgt gccgccccac
     3541 cgacgagcag ccgctgcgcg tgcagctgcg cgagaccgcc ggcgccgaca gcgaggcgat
     3601 cgagctcgcc gacatcgacg gcctcagcag cgagaccccc gccttcgccc gcctcgagcg
     3661 cacaggcgcg tgcgtccgcg gcctgacctc gatcgacggc atgcagtgga ccgagctcgg
     3721 cgcgcactgc ttccccgccc cgctgccgct cgtcggcctc accgcctcgt cgcacgacgc
     3781 cggtgaagtg cgcgtgttgt tcgtcgcccc gaccgcgagc cccggcggag cgctcgccgc
     3841 tggcacgctc accgtcacgc cggtcggtca ggccaagggc agcgtcgccg acgggctgtg
     3901 atacgccccg cgagcgtcga tccccagcag ctcgcgcagc tcgacgagcg gccgctccca
     3961 gtgctcctcg aagcgaaacg cgagcagcat ccgcgcgcgg cgagcgagcg cccgcccgcg
     4021 acggatcgca tcgcggatct gtcgccactg ccacggcttt cgcaatgtat agacaatgcg
     4081 cgccgagcgg agcagcagct cgctgctgcc cgagtagcgc tgctcggccg cgaatgcata
     4141 cacccccgcc tccccgggca gcgaggtgtc gaagccgagc acggtgtgga acatgtcatg
     4201 agtcgcgaca tatcgcaccg cgaaggtgtt gcgttgcacc tcctccgcca gctccgggct
     4261 gatcaccagc ggggagatgc cgttgtggtc gagaaaatcg acgtatgccc gaccgaagct
     4321 gcccggcggc aaatttctca agtaatcgac gtcgaccgct ggcatgtagc cgcgcaccgg
     4381 cccgagctgg gcttcgatct cgggcagcgc ccgggtcccc gccgcgtcgg ccttgagcac
     4441 cgccgcgtcg ccataccgtc ccgcacgaaa catggcgatc gctcgccgaa cctgtctcca
     4501 cgcgatcatg tcacatcctc ctgtcagcga tcccattcca cagcacatcg agcagcgaat
     4561 ctatcaactc cgccgtctcc tccggcgaga gccgggaggc gaggccggtg tcggccagat
     4621 attcgggtaa ggtcagcccg aggcgctcga gcagcgcgtt catcattccg tcgccgaggg
     4681 ccgcggtcag caccgccgcc gccggagcga gcgcgaggtc ggcccgcagc tcgcccgcag
     4741 cctgcgcgcc gcgcaacagc gcctcgagcc ccgcgcgacc gatcgcccgc atctcggcgt
     4801 gcagggcctg cagcgccggt tcgtcgcccg gcgcggccgc gtgcgcggcg accctcgcca
     4861 ggcgcgggtg cgtcaacgcg aagcgcagcc cggcgatgca catcgcccgc agccgcgtcc
     4921 gcaggtcgcc ctcgaccggc aatgcatcac cgatgaacgc ctgcttgcgc cgcccgacct
     4981 cctccacgac caaccagcga tagaggtcga acttgtcggt gaagtactga tatatgctgc
     5041 ccttggcgat ccccgcccgc gcgacgatgc gggacaggga ggcctgcgcg tacggccgct
     5101 gcgagaactc ctcgagcgcg agctcgacga tcctctcccg cttgtcggcc gccaggttta
     5161 agaaggtttg cgtcggcatc ggaggaccgt gactgaccag tcatgtgact ggccagtcat
     5221 gtctggcatg acgccgagcc cgatgcaagc ggtgctgaaa ttcgctcaat gaacgtcgtt
     5281 caacagcgat gacccgacag ggcgagaacg ccccagcggc gcgagaaaga gcgccctcga
     5341 cgcgcgattt tggcccggga atgccttcgt gaccaagcga ccgtgtcaca cgcgcggctt
     5401 cgccgtgtat cgattaagca tcgtggacga cgtttgcagc ggattttgcg gctcgatcga
     5461 gtgcatgtac ccgcggggat ccacctgatc tccactattt ttgcgaatag tttgccgttg
     5521 cagcggcaag aatctcgggc gtagagacgt ccgcatggct tggtgttcga tcgatcgcgt
     5581 cggcgtgggg gcgtgtcgcc tcgccgtgct ggcatcactc gcgggagcgg gctgcaggac
     5641 ctgggaggac gtcgccgtca cggagatgtt gtcggtgccg acggccgccg atgcgccctg
     5701 tgacgcgctg gcgcgcgatc tcgcggcgcc tggtgggtcg ctggcgatcg cggcgatcga
     5761 gttctcggac accaacgtgc tggcgctcga cgagaccctg gtgatccgcg cgagggtacc
     5821 gcactacagc gaatataccc gcaagctcga ccgcgaggag caggtcgacg gcttcgacct
     5881 cgagctgcag tgcccgcagt ggggcgaccc gacgccgctg acagtgcgca tgtgctcgga
     5941 ggatggaagc gcgttcggca tcgtcgacgc cgcccagctg cgccgattga ccgaccacgg
     6001 ggccctcggc gacacctgcc ggctgtgggg gcggctgtgg accggcgacc gcacgcgccc
     6061 gtggatggcc agcgtcatcg tgcgcccgtc cctgcggtcg ctcgtggagg tgcagtgatg
     6121 cgggccgccg tcgccgcggc gctgctcacc gggtgtgcac ataccagcgc gccgcgaccg
     6181 accccggtat ctgcacactt catggccgca tcggcggtcg aagattgcgt cgagatggcg
     6241 cgccggctcg accgcatcgg cgcgtgggcg ccgcgatatc gcaacgagcg cggcgaggag
     6301 gtcgcgcttt atccctcgga cgagctgtgg gtgctcaaca tggcgatggt cccgcccgcg
     6361 cgcgacggca gcgcggcgca ggggctgcgc tcgctgacgc tgacctgcgg ggccgagcag
     6421 cgcgcgctgc aggcggtcgc gtgtcgcaac ggcaccggcc tgcacctcta cgtcgatccg
     6481 gcccagctga ccggctggca gacgcgcggc acggtcgacg ccggctgccg cgtcgacgcc
     6541 gacgtcgcgg gcatcgtcta ccgcgacgag cgaaagatct tgctgcgtgc cggcctgcgc
     6601 gagcgcctcg gcccgatcga ccccagcccc aatcgctaca acgagccgcc cgcgcggacc
     6661 acccgcgtcg cgcgacctgt cgccgcggag tcgctggagt agccggacgt cattgtctag
     6721 aacgcgaatc caaggctgat cccggtcgcc tgcgaacgcg gcgagtacag cggggcgatg
     6781 ccacgcagct cgagccgcgg gcgcgtccgc gagatcttga gcttcttcgc ctcgtcgtac
     6841 agatcgcggc tcatgcggct gccgaccccg agcttgacgc caccgacgat ccccagcacg
     6901 agcccgccgg cccacatcgg cacggtggcg atcaggatca gctcatcgtc ggccgcccag
     6961 aacagcagac caccggcgac gatcgcaagg ccgccgctgg cgagcatcac ggcgccgccg
     7021 atgcgctttc gctgggcctt gcgggccatg tactcataaa aggtctcggg ctcgtcctgg
     7081 tcctcgggcc cctcggcggc gttctcccgc tgctccccct cgtaatccgc ccagtgccgg
     7141 cgaaagtcga agtcgacgcg cctgtgtaat cgcttcgccc gctcgtgctg gccagcgagg
     7201 tagccgtcga cgccgatgac ctgcggcggt gtcacgggca ccgcgaacgg gtcgacgggc
     7261 gcgggcgcct cggccgtcac cggtggcccg gaggggtcgc ccatcatcag cgcggatgac
     7321 acgagacaac cgacgaccgc ggacgcgtcc atggccgaag tatcgctcca cgctgccgcg
     7381 acccgtcgcc cggcgtctca cacagacgcg cggcatgacg cgcagcacag tgcacgcggc
     7441 cgcgcaggtc ggtccgcgcc ccagggggat gccagccacg gggacgcctg cgcacatccc
     7501 ggaagcggat cacgacgcga cggccgggct cagcgcggct gctggcggcg ggccgcgacc
     7561 caggcgaacg cctcggcctc gccgtcgacg tgctcggcgg cggtcagcgg ctgcttgctg
     7621 aacagcgccg acgcgcgcat cacgagcgcc tgcagcaggc ggatcggcag gctcgcgcca
     7681 aaggacacgt ggccggtgat cgggtgggcg cgggcccact cggagatcca cttgcgcgtc
     7741 gcggtcgccg gcacgcccgt gtggtgcatg tcgttgatga tgaacaggcg cccgtgctcg
     7801 gcgaacacgc gctcgaagtg acggtggatc tcctgcacgt gctcgagctc ggggacaccg
     7861 atgtagcgcg tgagcacggt gtcgccttcg acacgcagac gatgggggcc gatgagcagt
     7921 tccgacatgc agcggcgcgg agcatacgag acggcgaccc gcgggtctcg tgttcgccat
     7981 tgcggagcgt tgccacgccg aggtcgtcgc tcgccgagcg atcgctcgcg cgccagcgcg
     8041 cgggcgcgct gcttgtgtcg ccacgacggc ctcgctagtg tgccgcgatg ccggcccgcc
     8101 cgacctcgcc acgccgatgc ccccggatct ccgccctggc cgcgttgctg gtcctgtgcg
     8161 cctgcaacgg cgcgggctcc acggacaccg gcgcgagcac ggacgccagc agtggcaata
     8221 cgccgaccac cggcgcggac ccggacccca cgagcaccgg cggtgagccc gggccgacca
     8281 cctcgaccac gtcgaccacc tctaccaccg cggccacctc gaccacctcg acgagcgacc
     8341 cgaccacgac cagcggttcc gacactaccg gcgcgacgac gggcgaggac accggcgcgg
     8401 cacagtgtcc tccgccgctc gagcagccgc cgggcagctg gacctcggcg atcgtgccgg
     8461 tcggggatga cgacgcgctg acctacgtcg cggacgccga cggcaaccgg atccccgact
     8521 tcagctacgc cggctaccac cgcggcgagg cggcgatccc cgacgtgccg accgcgctcg
     8581 agctcggccc gatcgacggc gacgacaccg agcagatcca ggacgcgatc gacgaggtcg
     8641 gggcgatggc gcccggcccc gacggcctgc gcggcgccgt cgtgctcgcg cccggcgagt
     8701 atcagatcgc aggcacgatc gagctgcggc acagcggcgt cgtcctgcgc ggggccggcg
     8761 acggtggcga tcccacgagt gacacgatcc ttcaggccgt cggtgacacg ccgcaccagc
     8821 gcgtcgtgat cgtcgtcggc agcggcgaca acaaccggtg ggagcccgag ctgccgggca
     8881 cgcgcaccga catcgtcagc gagctggtcc agatcggcga tcgccagctc gaggtcgagg
     8941 acccgggcgc cttcgcggtc ggtgatcacg tcgtgatcgt gcacccctgc acggacgcgt
     9001 ggctcaccgc ggtcgaccac ggcgggaccg ccgacgacgg cccgtggacg gtcgattcgc
     9061 agccgatcgt ctttaaaaga cagctccttg cgatcgacgg cgcgacgctg acgctcgacg
     9121 cccccgtctt caaccacctc gaccgctcgc tcgcgcagtc gtacatctac gcgcaggacc
     9181 gaaagggtct cgtcaccgag gtcggcgtcg aggacctgcg cgtcgacatc gagaccgcgg
     9241 gcggcgagga cgaggaccac gcctggtcgg cgatcgccat gcgcggggtc gaggacggct
     9301 gggtgcgccg gttcaccgcg ctgcacttcg gcttcgccgc cgtcaccgtc cagaccggca
     9361 cgcagatcac ggtctccgac agccgcggca tcgacccggt cggcatcatc accggcgagc
     9421 gcatgtacaa cttcgacgcc agcggcggcc agcaggtgct gttcaccggc tgtcacgcga
     9481 gcggcggccg ccaccactac gtctccaacg gcacgacctg gacctcgggc gtggtgttcc
     9541 accgctcgac ctcgaccgac gcgcacgcct cgagcgaggg tcaccggcgg tggagcatgg
     9601 ggatgctgta cgacaacgtc gtcgagagcg cgccgagcaa ggacggcatg gtggtgctcg
     9661 ggctctacaa ccgcggcgac tacggcaccg gccacggctg ggcgtcggcg cactcggtcg
     9721 cctggaacta cgacaccagc accggcgtcg gcgcgatcca gcggccaccg accgcgcaga
     9781 actacgcgat cggcggcgcg ggcaagttca ccgggaacaa gccggccccg ttcgcgcagc
     9841 cggagggcta catcgagggc accggcaagc cgggcctggt gcccgagtcg ctgtacgacc
     9901 gccagctcgc cgagcgcctg tgcggcctgc gctgatccca cgagacttcg cgccgaagcg
     9961 gctcctgttc ccgacggcga cgcgcgcgcg caagcgaacg ctcatccctt ggacctcgcg
    10021 cgtgttaatc tcggccggtg gacccgcgcg ccgtactcga gccgaccctg cgctcgccgc
    10081 ccgcatggga cccggcggct accgtcgccg ggggtggcgc ggacgagacg atcgccggtg
    10141 caccggagac cgcggccgag ccgctcgagg cccgctttat cggtcacttc gcggtgctgc
    10201 gaaagctcgg cgagggcggc atgggggtgg tgtacgccgc gtacgacgag cggctcgagc
    10261 gacgcgtcgc gctcaagctg atccacggcg acgatccggt ccgtttgatg cgcgaggccc
    10321 aggcgctggc ccgcgtctcg cacccgaacg tcgtccagat ctacgaggtc ggggcctacg
    10381 ccgaccgcgt gttcgtggcg atggagtacg tcgaggggcc gtcgctcgcc gagtggctcg
    10441 ccgagccgcg tgacatcggc gcggtcctcg aggtcttcgt gcaggccggc cgcggactcg
    10501 aggccgcgca cgcgcgtggc ctggtccatc gcgacttcaa gcccggcaac gtgatcgtcg
    10561 gcgccgatgg ccgcgcacgg gtgctcgact tcgggctcgc ccgcgccgac gaccgccgcg
    10621 aagaagctgt ccccgcggac atctcggccg cgcgcacgac cgacgccaac ttgctcagct
    10681 caccgctgac gcgcaccggc tcgctgctcg ggaccccggc gtacatgtcc ccggagcagt
    10741 tcctcggcgt cccggtcgac gcccgcagcg accagttcag cttctgcgtc gcgctgtacg
    10801 aggcgctcta cgaccggcgg ccgttcgccg gcgcgacgat gccggagctg atggccgcgg
    10861 tgacgcacgg cgaggtcgag ctgcccccgc cacgcccgga gctggccgag acgatccccg
    10921 cggcggtcct gcgtggtctg gtcgccgagc cggaccgtcg ctggccgacg atgaccgagc
    10981 tggtcgacgt cctcgacgcc gcccgcgggc aggtcgacga gcgcagccgg gcgcaggccg
    11041 gcgggagcat cgcgtggatc atcaccatcg ccggcctcgc catgttcctc gcggtcaacg
    11101 tgctctggga cccgggcgac gtgacgcccg cctcgatgac ccgcttcgcc gtgatgatcg
    11161 cgctggtcgc cgtcgcggcc ctgccgaccc tgcgcgcccg ctaccgcgag ccccagcagc
    11221 gggtctacgt caacttcggc gtcgccatgc ccgcgatgag catggtcgcc cgcttcttct
    11281 cgtggcgcgc cggcctgtcg atgagcgaca tggcggtcac cgaatcgctg tgcttcgtca
    11341 ccgcctatat gacgatggcc gtgttcctgc gcgcgccgct ggtgttgatc aacataccgg
    11401 tgctgctcgg cgcgctcgtg ctggtccacg agctcggcgc cccggggcag atcctcgcgc
    11461 tgtcgtggct gctcagtatt ccggtgaccg cggcggcgtg gtggtacacc gccttgcgcc
    11521 cgcgccggcg cggcgccacc tcgcagacct cgatgtcggc ggggccggtg atgccctcga
    11581 tcaacacgct gacgcgatga cgctggatca gctcgcggtc gaggaagctg catgaccggg
    11641 cagcgaccgg acctggccgt ccggaccggc cgttgtgcca ccgtacgccc gaagcggtga
    11701 cgatcagctc agggcggcgt agcgagagtg cgggggcccg gacggcgggg cgacatccgg
    11761 gaacctatca tcttcagtcg cggacatccg gcgggcaggc aggcgcggcg gcgctcgggg
    11821 gttgacgtcg ttgcgccccc ctcgcacacc acccggccga cctcggcagg acaacgaaca
    11881 gggtccacgg aagatcgagt tgtcggcgca gcccgacgcg acgacgacgc cgagcatccc
    11941 aagggctcta cacagtcggt tccgcggcga ccgttgtttt gaaccgaccg gatagcgcat
    12001 tcagcgcagt ggcgcgcgag caaaaagcgg gcagaccgtt accacgatga gcaaactttt
    12061 acgcggtgca gttgcgggtg caggggcttg gaagctcggc ggcggtttca ttggcacgat
    12121 cctgctgttc atccttttgt gggtcgtcct cggcaatttc gacatcttca agtgatcgtc
    12181 agcaaaagtg gccgggccgc ccccgtggta gcgatacctc ggggctgcgc atgaccttcg
    12241 acccgcgacg tcgctgcatt tcgcggggta cgatcggagc cgagccaggc taaaaccgtc
    12301 cacgacctcc cggccgcgcc ggctgtcatc cccaggtgtc cccaggagcc cagcgtcggc
    12361 aggtggctac agcttgccct tgagcccagc gggcgggtgc aggcccgctc gctgcctgta
    12421 ccgcataaag tgcggagcac ggcgctcgac gagcgacggc gctcgacgcc ggcgcttgca
    12481 ctgcgcttgc gcgcttgtgc tcggcgggcg ggctcgcgcg gcaactcgct tgcaccgccc
    12541 tctcgcgacg ctgcgcccgg cgtgggcacg cgacctgcgc ggcctggcct ttcagacatc
    12601 ttaaacattt tagacttctt ctttagaagc tttagataag ccgctgctcc ttctttaggg
    12661 cttatcttct taaaaattcg tcgtatggtt gtaaggacag gccgcgcagg tggttgtaag
    12721 gacaggtcgc gcatggctct gaacttcggc gcaggtgcct cgctcggtgg cgccgccgtg
    12781 cctgcgcgtc tgcataccat cgcgcagcgc ggacgggcct ccgcggtcgc gcgtgcaggg
    12841 gccgtcgcgc ggttccggtg tcttgtgggc cccacgtctt ctcgatatca aggtgatcac
    12901 tggtagcgat ctgaaaagga gacagcactc ttcaggtcgg cgatgcaatc cttttggaga
    12961 gaaatcttga agaactacga ccataccacc ccccatcggc gtgcaaactt cgcatttgca
    13021 gtgtatgcgg ggattcaggt cgcctgcagc gccgattcgc cggtcaccag ccagcccact
    13081 catccgagcg cgaccggaac gaccggcgct acgagttccg gcgggctcac tggcgccacg
    13141 gaggcggata cctccatgat accaacaacc tccatgacac cggcggagag ctcgaacacg
    13201 acgatgcaga cctccgacgg ctcgacatct tggtctggca cttccgacgc gaccaccgcc
    13261 tcgtctggca cttccgacgc gaccgccgcc tcggagtcct cgaccggagc ggcgcatgat
    13321 tggagcctcg aagttgagga catcagtccc ggcctgggca ttcatatcga cggacccatg
    13381 caactcgtcg ggataggtga tcacttcgca tacgtcgacg gttgggggca ggacggcgtt
    13441 agtgaccttg tcataaacgc ccgttttggg ctcgtggacg gcaattactc aagttcgttc
    13501 gatctcgcgg acgtcgagaa atacgcgacc gcggtcctga ttaccgtagt tgggggattt
    13561 cgtggcgcgt atgccagcgt cggcgacatt aatggtgaca cgatcgagga tttgatcatt
    13621 ggggagcccg gctactacat cgagggctac gacgaggact atctcggaga gggccggtgt
    13681 catctcgttt ggggcccggc ggagctggaa gacatcgatg tgccctcgct tttcgagcag
    13741 ggcctaggtt ccagggtcac gggcgtcgat tatgggcagg aggtcgggcg gcgcgtggct
    13801 agcgcagggg acgccaatgg tgacgggctc tctgatacgg tgatgacgac ggtcgggaac
    13861 tcacgcagtg cgttggtgtt cggcgacgct tctcccatga gcctcagtac actcgatatc
    13921 gaggcaggaa tgggtgggta ccccattgaa gcgccagcca gctcgctgct gctgcctctc
    13981 ggggatgtcg acggcgacgg actcgcggac cacacccaga tcggtagtag tcagatggga
    14041 agcaccatca gtatcatcta cgggaagaac gacgtggaac cggtaacctt ggacgggcta
    14101 aaggtcggcg agggtggttt catcatcgat gtctccactc tatactcgtc gggtgcggac
    14161 tttggggtaa gtggggcgtc tctcggcgac ttcgacggcg atggttggca tgatcttgtt
    14221 gtcacggccg gggtcgcccc agcggctcga agaatcttca tgatccgtgg cgcagaatcg
    14281 tggggacaac ccaccctcgc cactctccag gccgaggacc ggatccggct ctttgaaggc
    14341 ccggattatc ccgggggggt gctttcattt gtggacgatc tcaatggcga tggacgcgac
    14401 gaattgctcg ccggctggcc gttctccaac accgtatatc tagtattcgg gcaggagggc
    14461 acccatacga tctctctgga atcactcgtg gaggacaaga cgggagtgaa attccgtaat
    14521 acagtgggtc acattggact cggcaggggg ctgatcggaa cgagggacct cactggggac
    14581 gggatcgccg agctcgtcct cggtgctcca caggcgtcgc cgatgggccg ccctggtgcc
    14641 ggccaagtat tcattgtaga cggcgcggcg ctggtggctt ggctatgacg cccggcctcc
    14701 gacgacctcg ctgtgcctgc ccctcgcggc ccaagggcca ggtagttgtc gcgctcgacg
    14761 aagacgagag cagtttgcgg ctccggcggg tgcccgccta gtactacttt ctcgggtgcc
    14821 gcttgatttc gcgcggagcg agtttatgct tcgggcatga ctccgcggca acttcagaag
    14881 ctggatcaag agcttcgggc gttcgtcggc g
//
LOCUS       DASWNJ010001898         1776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_33384, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001898 DASWNJ010000000
VERSION     DASWNJ010001898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33384"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VGB85_31645"
     CDS             complement(<1..528)
                     /locus_tag="VGB85_31645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138696.1"
                     /translation="MPTPTRDDPTPADAVLLLAFFLSGAAALGYELLWTRLLALSLGS
                     ETLGVLATLAGFFGGMAIGAAALHRRAETCEDPVRLFVTLELVAAGFAIVSPYLLHAL
                     AGVIPPLLGPLAGAGNSPGALLASLLVATLALLPGTVCLGATLAALATARRRAHASDP
                     AGRGLGRLYAANTAGA"
     gene            617..>1776
                     /locus_tag="VGB85_31650"
     CDS             617..>1776
                     /locus_tag="VGB85_31650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0138697.1"
                     /translation="MKIAVIGGGPAGLYFALLMKKARPEYEVVVVERNRADDTFGWGV
                     VFSNETLGHFRAADPESFARITEAFAYWDNIDTHFKGQVIRSGGHGFCGIARRRLLQI
                     LQERGRELGVDLRFSTEVEELGELAGYDVVVAADGINSRIRGAHAEVFKPTIEPGKAR
                     FIWLGTTRRFEAFTFLIRDTEHGLFQVHAYPFDQATSTFIVETDPDTWRRAGLDTASV
                     EHSVAYLEALFAPELAGHRLLTNRSAWINFRTISCERWSHGNVVLIGDAAHTAHFSIG
                     SGTKLAMEDSIALVRALEQRPDDIPGALAAYHEARWLDVAKLQRAARVSQAWFEDISR
                     YKHMAPEQLVLSMMTRSKRVTHGNLRVRDANYVAGVDRWFAESTGAEVGPPM"
BASE COUNT          279 a          582 c          674 g          241 t
ORIGIN      
        1 ggcgcccgcg gtgttggcgg cgtagaggcg gccgaggccg cggcccgcgg ggtcgctggc
       61 gtgggcgcgg cggcgggcgg tggcgagggc ggccagggtg gcgccgaggc acacggtgcc
      121 gggcagcagc gcgagggtgg cgacgagcag gctcgcgagc aaggcgccgg gtgaattgcc
      181 ggcgccggcg agcgggccga gcagcggcgg gatcacgcca gcgagcgcgt gcaggaggta
      241 cggcgacacg atcgcaaagc cggcggcgac cagctcgagc gtgacgaaca ggcgcaccgg
      301 atcttcgcag gtctcggcgc ggcggtgcag ggcggccgcg ccgatcgcca tgccgccgaa
      361 gaagccggcg agcgtggcga gcacgccgag cgtctcgctg ccgagcgaga gagccagcag
      421 gcgggtccac agcagctcgt agccgagagc ggcggcgccc gagaggaaga acgcgagcag
      481 cagcacggcg tcggcgggcg tggggtcgtc tcgcgtcggc gtgggcatgc ggcgatgcga
      541 gcatacgcgt gggccgacgt gtgccgtcgt ggggcttctc acttgcagcg aggcgaggcc
      601 cgtttaccat gccgccgtga agatcgctgt catcggtggc ggaccggcgg gcctgtattt
      661 tgcgctcttg atgaagaagg cgcggccgga atacgaggtc gtggtcgtcg aacgcaaccg
      721 tgccgacgac accttcgggt ggggcgtggt gttctcgaac gagacgctcg gccacttccg
      781 tgccgcggac ccggagagct tcgcgcggat cacggaggcc ttcgcgtact gggacaacat
      841 cgacacccac ttcaagggcc aggtgatccg ctccggcggg cacggcttct gtgggatcgc
      901 ccggcgaaga ctgctgcaga tccttcagga gcgcgggcgg gagctcgggg tcgacctgcg
      961 cttctcgacc gaggtcgagg agctcggaga gctcgcgggt tacgacgtcg tggtcgcggc
     1021 ggacggcatc aacagccgga tccgcggcgc gcacgcggag gtgttcaagc cgacgatcga
     1081 gccgggcaag gcgcgcttta tctggctcgg caccacgcgc cgcttcgagg cgttcacgtt
     1141 cctgatccgc gacaccgagc acgggctgtt ccaggtccac gcctatccct tcgaccaggc
     1201 gacgagcacc ttcatcgtcg agacggaccc cgacacgtgg cggcgcgcgg gcctcgacac
     1261 cgcgagcgtc gagcacagcg tcgcgtacct cgaggcgctg ttcgccccgg agctcgcggg
     1321 ccaccgcctg ctgaccaatc gttccgcgtg gatcaatttc cgaacgatct cctgcgagcg
     1381 ctggtcgcac ggcaacgtcg tgctgatcgg cgacgcggcg cacacggccc acttctcgat
     1441 cggctccggc accaagctgg cgatggaaga ttcgatcgcg ctggtgcgcg cgctcgaaca
     1501 gcgacccgat gatatccccg gggcgctggc ggcgtaccac gaggcccgct ggctcgacgt
     1561 cgccaagctg cagcgggccg cgcgcgtcag tcaggcgtgg ttcgaggaca tctcacgcta
     1621 caaacacatg gcgcccgagc agctggtgct cagcatgatg acccgcagca agcgcgtcac
     1681 ccacggcaac ctgcgcgtgc gcgacgcgaa ctacgtggcc ggcgtcgacc gctggttcgc
     1741 cgagagcacc ggcgcggagg tcgggccgcc gatgtt
//
LOCUS       DASWNJ010001899         9278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_456038, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001899 DASWNJ010000000
VERSION     DASWNJ010001899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 9278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9278
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456038"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="VGB85_31655"
     CDS             complement(<1..892)
                     /locus_tag="VGB85_31655"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY0138698.1"
                     /translation="MNKLAAVTLVPSLLAALVVLPSCDVPLQEVYPLQSQTSLPEGVA
                     YDTVTHTFFATAINGGKITRITPLGQEIVFHSDANPNMSFGGAHVDEDHRRLWVCAVD
                     VKTNPFPTSQVWAFNIETKKRTHVIPLPPVSFCNDLVTDATGHVYVTDSANPNIYLVN
                     PKTQSFSVFLTSPTFNPVQPGMPALNGIDLSPDGDRLLAVTTFPAGVYSIPVADPQSF
                     VAVQTSGDPFAMPGDPRFPGPDGAEFHDGRLYVAYDGGVQALSFADPDFTQATVATTT
                     AVPTGLTSLTVAEDQLYAIDS"
     gene            1665..2297
                     /locus_tag="VGB85_31660"
     CDS             1665..2297
                     /locus_tag="VGB85_31660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138699.1"
                     /translation="MPAPAISPPPRLQLSAGFVFGPHALGEADCQSNDGISECQHRGN
                     FLGAGATIELRGQLYKFLYLHARGVVVGNVRQRPYAVHSGLAGGGLGLGVYSRLAFIR
                     GEYMLIPAFGPDTYVPPFYNKQAASDRYGLHAGMISGGIHKYVTPRTALELWAGFIVG
                     PTAERTSLSMQVNEKRLLKTFMVSVGFVFDLVLAKGYVPPPKQPRKRRVW"
     gene            complement(2310..2966)
                     /locus_tag="VGB85_31665"
     CDS             complement(2310..2966)
                     /locus_tag="VGB85_31665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0138700.1"
                     /translation="MDAPTSPLEVVQLEVGLLQNFCEVIGCPRTGEAALVDPAFEVDR
                     LLREVQRRGWRVTTIFLTHTHDDHVAGLDEAAAATGAVVRCHPLEVKAALVHAHRVEA
                     VADDEWVPLGASAVRAIHAPGHTPGCVCWFIPEPAAIITGDVLFVGSCGSAPDPRAML
                     DTLQRRLGALPEHTRLYPGHDYGKTPTSTLAWEFAQSPALRADTLPAFCTYKKQPLPR
                     "
     gene            3075..5147
                     /locus_tag="VGB85_31670"
     CDS             3075..5147
                     /locus_tag="VGB85_31670"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY0138701.1"
                     /translation="MDLSALNPDQRAAVMATEGPLLVLAGAGSGKTRVITHRVVYLLE
                     RGVPPESIVALSFTNKAADEMRDRLRKMVGKQGDALVLGTFHSLGAAMMREQPDQFGV
                     PKRFSILDQSDVYGVTRTVLRELGHNGAGADRRFDLGAVVQRISLWKNEFILPEEALE
                     LDHETDYDEVAAACYAPYEERLRSLGSVDFDDLITRVAHVLEDDDAARAYWQAKFQYL
                     MVDEFQDTNRAQFEMVRRLASGRENLCVVGDDDQAIYGWRGAKVTYILGFDMYFARPD
                     DVKTIKLEQNYRSYAPILRCANELITNNRTRHDKQLVPQRRGGGPVQLVVAADGDQET
                     RWIGKKIYKLIHDEGVRPEDIAVLYRSSRQCDPVEALLQEHGIAYRVLGGQAFYDKKQ
                     VKDVLAYLKAIVAPQDDLAVRRAVDVPSRGVGLKTIEYLSDFARRHNLSLMEAIHRST
                     EIPEIPGRAGPALAQFSAQIKRGQAHFYASGSALGALRSVVDNVGLKAAMLKENGGSE
                     AAAAKWDSVEWMYESLGRFEQRAKNKGGRPRWNDFFQAMNQDNKQDEPDAAGRGQVTL
                     ATMHSAKGLEWPHVFIIGCEEGTMPHKRVAAPRISDAIAGDIEEERRLFYVGITRARD
                     VLYLTRAATRLERGAEVPRKPSRFLEELPEKDLKVYEIAREERLTGQDVQSMAEAFLA
                     KLGVTSAG"
     gene            complement(5219..5575)
                     /locus_tag="VGB85_31675"
     CDS             complement(5219..5575)
                     /locus_tag="VGB85_31675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138702.1"
                     /translation="MRWFVAVLVLLAGCRDQPVEARRAEVETMCAQFCEDRIACVADG
                     YATGSVQECERKCLGDERPLEDNACGEASFAALECLTAVACEDLAMAVAAVDPDAACF
                     AELRAQQDECDFTPLY"
     gene            complement(5572..6438)
                     /locus_tag="VGB85_31680"
     CDS             complement(5572..6438)
                     /locus_tag="VGB85_31680"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEY0138703.1"
                     /translation="MCRIFGFRSVMQSQVHRSLVSADNAFMRQSERHPDGWGVAYYLA
                     NAPHVIKGASAAFGDQLFKHVSGIVTSETVLAHLRKATQGELSTINTHPFQYGHWVFA
                     HNGNIEGFADIREALLDKIPPVLRRFILGTTDSEVLFYLILGKMAQRCELHRPGFPLA
                     DIAESVREAVAEITALAGPVCEDNAGPPTKTYLTFVLTNGHAMLAHQGGKELFFSTHK
                     RRCPERDTCPHFAPECERAADNGFISHLVLSSEPLHGDNVWEQMQPFEMIAADARMQV
                     QRFAPSLPTGAA"
     gene            6644..8488
                     /locus_tag="VGB85_31685"
     CDS             6644..8488
                     /locus_tag="VGB85_31685"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY0138704.1"
                     /translation="MQTTFEALVSNAQHFKSNLRDIFFNLFEVLGIDKTILGKGEFAG
                     QDEETVRATLEAYDEFCAKELAPSWAASDRIPLTLDADGNVHLPEPLKRSLKAHHDNE
                     WHLMGLPERIGGTGAPSTLAWSSFELTAAANAAAAFYMLGTVIARVIEELGTDDQKQR
                     FLTPMIERNWGASMVLTEPDAGSDVGAGRTKARHVEGEIYELEGVKRFITNGDYDGPE
                     NIIHLVLARPEGAGPGTKGLSMFIVPKFWVNEDGSLGERNGVYCTNIEHKMGLKASAT
                     CEMTFGERGPARGLLVGGVHEGIRQMFHVIEHARMAVGCKSMATLSTAYLNALEFTKI
                     RVQGADLLRANDKTAPRVRIIEHPDVRRMLMLQKCFAEGMRALCLFTASIQDEVTLAG
                     GHGSAAAAELDRLNDLLLPLVKGYCSEKVYELLATSLQCYGGSGYVQDYPIEQYIRDQ
                     KIDTLYEGTTHIQALDLFFRKVARDGGETLRGLMGRVMATLGEIDANGPLAVEHAALS
                     RALGDLQGMYVAMLGKLGESMYHVGLQGNRVLFATAEVVIGWLLVRQAAVAAAARETA
                     RDGERAFYDGKLASVRFYARNVLPGITLARKLCEQSTLELMEVPEAAF"
     gene            8546..>9278
                     /locus_tag="VGB85_31690"
     CDS             8546..>9278
                     /locus_tag="VGB85_31690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138705.1"
                     /translation="MDEALSILRALLDEHADEVVDEGTAWVRAESQADLSARPWAETR
                     SLVAQCLAFYRAHLFDGDTAPREAFIELVTSNRSNLKFSVSTLLRGFMSFRVAVVHLV
                     ARMEVPPALALTITRKVDEVSAEASFRTADLYVAKLNRVIDATREELIRKDKLAALGG
                     LVAGVAHEINTPLGIAVTASSLVADRLRDLYEAFEAGTLRRQDLQNGLVQAQEAARLT
                     LGNLRRAADMVGNFKQIAVDQTSEAR"
BASE COUNT         1492 a         3141 c         3281 g         1364 t
ORIGIN      
        1 cgctgtcgat cgcgtagagc tggtcctcgg cgaccgtcag gctggtgagg ccggtcggca
       61 ccgcggtcgt cgtggcgacc gtcgcctgcg tgaagtcggg gtcggcgaag ctcagcgcct
      121 gcacgccgcc gtcgtaggcg acatacaggc ggccgtcgtg gaactcggcg ccgtcggggc
      181 ccgggaagcg cgggtcgccc ggcatcgcga aggggtcccc cgaggtctgc acggcgacga
      241 agctctgcgg gtcggcgacc gggatgctgt acacgcccgc cgggaaggtc gtgaccgcga
      301 gcaggcggtc gccgtccggc gagaggtcga tgccgttgag cgcgggcatc ccgggctgga
      361 ccgggttgaa cgtcggcgac gtcaggaaca ccgagaagct ctgggtcttc gggttgacga
      421 ggtagatgtt cgggttggcc gagtcggtga cgtagacgtg gccggtggcg tcggtgacga
      481 ggtcgttgca gaacgacacc ggcggcagcg ggatgacgtg cgtgcgcttc ttggtctcga
      541 tgttgaaggc ccacacctgc gaggtcggga aggggttggt cttcacgtcg acggcacaga
      601 cccacaggcg gcgatggtcc tcgtcgacgt gcgcgccgcc gaaggacatg ttggggttcg
      661 cgtcgctgtg gaacacgatc tcctggccga gcggcgtgat gcgggtgatc ttgccgccgt
      721 tgatcgccgt ggcgaagaac gtgtgcgtga cagtgtcata agcgacgccc tccggcagcg
      781 aggtctggct ctgcagcggg tagacctcct gcagcggcac gtcgcacgac ggcagcacca
      841 cgagggcggc gaggagagag gggaccaggg tgacggcggc gagcttattc atggggactc
      901 ctccaggagt gggcagctcc aggggcttcg atcaggcgca gatacaaaca tgggcggctt
      961 atccagagca gatcagccac cagcgcgacg cggtattgac cgggcgcgga tcggaatagg
     1021 gcctaaaagc gcgccagaag cttgtgcagg gcgacctgga ccagaacggc caggtaagaa
     1081 ctttcttttt actcaagcaa gcggtgtcag atcaagatcg atgaccgcgc gtcgcagatc
     1141 cgcgagtgat cacccggacc tgcatcggac ctgcgccgac acgcctcgag atccccggat
     1201 catggcgacc ttgcacgcat gaggcgccct gcgcattcca tcgacccaca tcgcgacgac
     1261 tggccattgt cgcccggtcg aggcacagtc tggacatgcg ccgcgcccgt acgctgcccg
     1321 ccctcgtcgc gtcgttcctc gcgggaccgg ccctggcaca cgcgcagggc cccgatccgg
     1381 ccgccggggc cacgacgagc gcggcgaaca catctgccac tcccaacgct tcgagtacat
     1441 ccacatcctc atccacgtca cccacgtcac ccacgtcatc cgctccatcc gctccttccg
     1501 ctccttcatc cgcagccgcg ggtgtgccca cggagccagc gcccgcgtcc gctccggccg
     1561 cgagtgacgc ggtgaccgcg acgccggcgt cggctccctc acccgcgacc acaccgggcg
     1621 atcggccgcg ggcggcgccg cccgggacag ttgcgccccc cgccgtgccc gcgccggcga
     1681 tctcgccgcc tccgcgcttg cagctgagcg ccggcttcgt gttcggtccg cacgcgctgg
     1741 gcgaggccga ctgtcagagc aacgacggca tctcggagtg ccagcaccgc ggcaactttc
     1801 tcggcgcggg cgcgacgatc gagctgcgcg gccagctgta caagtttctg tacctccacg
     1861 cgcgcggggt ggtcgtcggc aacgtgcgcc agcgaccgta cgcggtgcac agcgggctcg
     1921 cgggcggcgg gctcgggctc ggcgtgtact cgcgcctcgc gttcatccgc ggcgagtaca
     1981 tgctgatccc cgcgttcggg cccgacacct acgtgccgcc gttctacaac aagcaggccg
     2041 cgagcgaccg ctatggcctg cacgccggca tgatcagcgg cggcatccac aagtacgtca
     2101 caccacgcac cgcgctcgag ctgtgggccg gcttcatcgt cgggcccacc gccgagcgca
     2161 ccagcctgtc gatgcaggtc aacgagaagc gcctgctcaa gaccttcatg gtcagcgtcg
     2221 gcttcgtgtt cgacctcgtg ctcgcgaagg gctacgtgcc gccgccgaag cagccgcgca
     2281 agcgccgggt gtggtaatcg gcgcagtagt cagcgcggca gcggctgctt tttgtaggtg
     2341 cagaacgccg gaagcgtgtc ggcccgcagc gcggggctct gcgcgaactc ccacgcgagc
     2401 gtgctcgtcg gcgtttttcc ataatcgtgg cccgggtaca ggcgcgtgtg ctcgggcaga
     2461 gcgccgaggc ggcgctgcag ggtgtcgagc atcgcgcgtg ggtcgggcgc gctgccgcag
     2521 gagccgacga acagcacgtc gccggtgatg atcgcggcgg gctccgggat gaaccagcac
     2581 acgcagccgg gcgtgtgtcc cggggcgtgg atcgctcgca ccgcgctcgc gccgagcggg
     2641 acccactcgt cgtcggcgac ggcctcgacc cgatgtgcgt gaacgagcgc ggccttcacc
     2701 tcgagcgggt ggcagcggac gaccgccccg gtcgcagcgg cggcctcgtc gagcccggcg
     2761 acgtggtcgt cgtgggtgtg cgtgaggaag atcgtcgtca cgcgccagcc gcggcgctgc
     2821 acctcgcgca gcaggcggtc gacctcgaag gcggggtcca cgagggccgc ctcgccggta
     2881 cgcgggcagc cgatgacctc gcagaagttt tgcagcagac cgacctcgag ctgcacgacc
     2941 tccagcggcg acgtcggcgc gtccaccgga ggaccataga cacacccggc cgcgtgatac
     3001 aaggccttgc ggacgcggcg tcgcgggtct tacaaccgcg agctaccacc gcggttcgtt
     3061 cgcggcgaca cacgatggat ctcagcgcac tcaatccgga tcagcgcgcg gccgtcatgg
     3121 cgaccgaggg cccgctgctg gtgctcgcgg gcgccggctc gggcaagacg cgggtcatca
     3181 cgcaccgcgt cgtgtacctg ctcgagcgcg gcgtgccgcc cgagagcatc gtcgcgctga
     3241 gcttcaccaa caaggctgcc gacgagatgc gcgatcgcct gcgcaagatg gtcggcaagc
     3301 agggtgacgc gctggtgctc ggcaccttcc acagcctcgg cgcggcgatg atgcgcgagc
     3361 agccggacca attcggggtc cccaagcgct tctcgatcct cgaccagagc gacgtctacg
     3421 gggtgacgcg cacggtgctg cgcgagctcg gacacaacgg cgcgggcgcc gaccgccgct
     3481 tcgacctcgg cgcggtggtg cagcgcatct cgttgtggaa aaacgagttc atcctcccgg
     3541 aggaggcgct ggagctcgac cacgagaccg actacgacga ggtcgccgcc gcctgttacg
     3601 cgccctacga ggagcggctg cggtcgctgg gctcggtcga cttcgacgac ctgatcaccc
     3661 gcgtggcgca cgtgctcgag gacgacgacg cggcgcgggc gtactggcag gccaagttcc
     3721 agtacctgat ggtcgacgag tttcaggaca ccaaccgggc gcagttcgag atggtgcgcc
     3781 gtctggcgag cgggcgcgag aacctgtgcg tcgtcggcga cgacgaccag gcgatctacg
     3841 gctggcgcgg cgccaaggtg acctacatcc tcggcttcga catgtacttc gcgcgcccgg
     3901 acgacgtgaa gacgatcaag ctcgagcaga actaccgctc atacgcgccg atcctgcgct
     3961 gcgccaacga gctgatcacc aacaatcgca cgaggcacga caagcagctg gtcccgcagc
     4021 gccgcggcgg cggcccggtg cagttggtgg tcgccgccga cggcgaccag gagacgcgct
     4081 ggatcggcaa gaagatctac aagctgatcc acgacgaggg cgtgcggccg gaggacatcg
     4141 ccgtgctgta ccggtcgtcg cgccagtgcg acccggtcga ggcgctgctg caggagcacg
     4201 gcatcgcgta ccgcgtgctc ggcggacagg ccttctacga caagaagcag gtcaaggacg
     4261 tgctcgcgta cctcaaggcg atcgtggcgc cgcaggacga cctcgcggtg cgtcgcgcgg
     4321 tcgacgtgcc gtcgcgcggc gtcggcctca agacgatcga gtacctctcg gacttcgcgc
     4381 gccgccacaa cctgtcgttg atggaggcga tccaccgcag caccgagatc ccggagatcc
     4441 ccgggcgcgc cgggccggcg ctcgcgcagt tctcggccca gatcaagcgc gggcaggccc
     4501 acttctacgc cagcggctcg gcgctcgggg ccctgcgcag cgtcgtcgac aacgtcggcc
     4561 tcaaggcggc gatgctcaag gagaacggcg gctcggaggc cgccgccgcc aagtgggaca
     4621 gcgtcgagtg gatgtacgag agcctcggcc gcttcgagca gcgcgccaag aacaagggcg
     4681 gccggccgcg ctggaacgat tttttccagg cgatgaacca ggacaacaag caggacgagc
     4741 ccgacgcggc cggccgcggt caggtgacgc tggcgacgat gcacagcgcg aagggcctcg
     4801 agtggccgca cgtgttcatc atcggctgcg aggagggcac gatgccgcac aagcgggtcg
     4861 ccgcgccgcg catcagcgac gcgatcgccg gcgacatcga ggaggagcgg cggctgttct
     4921 acgtcggcat cacgcgcgcc cgcgacgtgc tgtacctgac gcgcgccgcg acccggctgg
     4981 agcgcggcgc cgaggtcccg cgcaaaccga gccggttcct ggaggagctg cccgagaagg
     5041 acctgaaggt ctacgagatc gcccgcgagg agcggctgac gggccaggac gtgcagtcga
     5101 tggccgaggc gtttcttgcc aagctcggcg tcacttctgc gggctgagat cgcacagagc
     5161 cgtgcggtgt tcggccggcc cctgcgtcgc gccgcctttt atgaagttcg ccgggccgtc
     5221 agtacagcgg cgtgaagtcg cactcgtcct gctgcgcgcg cagctcggcg aagcaggcgg
     5281 cgtcggggtc gaccgcggcg acggccattg ccaggtcctc gcaggcgacc gccgtgaggc
     5341 actcgagcgc ggcgaacgag gcctcgccgc aggcgttgtc ctcgagcggg cgctcgtcgc
     5401 cgaggcactt gcgctcacac tcctggacgc tgccggtcgc gtagccgtcg gcgacgcagg
     5461 cgatgcggtc ctcgcagaac tgcgcgcaca tcgtctcgac ctcggcgcgg cgcgcctcga
     5521 ccggctggtc acggcaaccg gccagcagca ccaggaccgc cacgaaccac ctcacgcggc
     5581 ccccgtgggc agcgacggtg cgaagcgctg gacctgcatg cgggcatccg ccgcgatcat
     5641 ctcgaagggc tgcatctgct cccacacgtt gtcgccgtgc agtggttcgc tcgacagcac
     5701 gagatggctg ataaagccgt tgtcggccgc gcgctcgcac tcgggggcga agtgcgggca
     5761 ggtgtcgcgc tcggggcagc ggcgcttgtg cgtgctgaag aacagctcct tgccgccctg
     5821 atgcgcgagc atcgcgtggc cgttggtcag cacgaaggtc agataggtct ttgtcggcgg
     5881 gccggcattg tcctcgcaca cggggccggc gagcgccgtg atctcggcga ccgcctcgcg
     5941 cacggactcg gcgatgtcgg ccagcgggaa gcccggacgg tgcagctcgc agcgctgcgc
     6001 catcttgccg aggatcagat agaacaggac ctcgctgtcg gtcgtgccga ggatgaagcg
     6061 gcgcagcacc ggcgggatct tgtcgagcag cgcctcgcgg atgtcggcga agccctcgat
     6121 gttgccgttg tgggcgaaca cccagtggcc gtactgaaac gggtgggtgt tgatcgtcga
     6181 gagctcgccc tgcgtcgcct tgcgcaggtg cgcgagcacg gtctccgatg tgacgatgcc
     6241 cgagacgtgt ttaaaaagtt ggtcgccgaa ggccgccgag gcgcccttga tcacgtgggg
     6301 cgcgttggcg aggtaatacg cgacgcccca gccgtccgga tggcgctcgc tctgccgcat
     6361 gaacgcgttg tcggcgctga cgaggctccg atgcacctgg ctctgcatga cggagcgaaa
     6421 tccgaagatc cggcacatgg cgtggccgca agcgtactgc gaaacgccgc ctggcggcac
     6481 cgcggtggcc agatcggcga aagttcgcgt gcgagggccg gccctcgtac gcccggccgg
     6541 cccaggctac aacggccgcg gtccgcgcgt ggacgttgac aagccccgcg aaatctgaaa
     6601 actgccgtgc cgtggcgtcc gcgtgtgggg gccacggtag tcggtgcaaa caacgttcga
     6661 ggccctcgtg agcaacgccc agcacttcaa gagcaacctg cgcgacatct tcttcaacct
     6721 cttcgaggtg ctcggcatcg acaagacgat cctcggcaag ggtgagttcg cggggcagga
     6781 cgaggagacc gtccgcgcca cgctcgaggc ctacgacgag ttctgcgcga aggagctggc
     6841 gccgagctgg gcggcgagcg accgcatccc gctgacgctc gacgccgacg gcaacgtgca
     6901 cctgcccgag ccgctcaagc gctcgctcaa ggcgcaccat gacaacgagt ggcacctgat
     6961 gggcctgccg gagcgcatcg gcggcacggg ggcaccgtcg acgctggcgt ggtcgtcgtt
     7021 cgagctgacg gcggccgcca acgcggcggc ggcgttctac atgctcggca cggtgatcgc
     7081 ccgcgtgatc gaggagctcg gcacggacga ccagaagcag cgcttcttga cgccgatgat
     7141 cgagcgcaac tggggcgcgt cgatggtgct caccgagccg gacgccggca gcgacgtcgg
     7201 cgccggtcga accaaggcga ggcacgtcga gggcgagatc tacgagctcg agggcgtcaa
     7261 gcgcttcatc accaacggcg actacgacgg cccggagaac atcatccacc tcgtgctcgc
     7321 gcgccccgag ggcgccggtc cgggcaccaa gggcctgtcg atgttcatcg tgcccaagtt
     7381 ttgggtcaac gaggacggct cgctcggcga gcgcaacggg gtgtactgca ccaacatcga
     7441 gcacaagatg ggcctcaagg cctcggcgac ctgcgagatg accttcggcg agcgcggacc
     7501 ggcgcgagga cttttggtcg gcggcgtgca cgagggcatc cgccagatgt tccacgtcat
     7561 cgagcacgcg cgcatggcgg tcggctgcaa gtcgatggcg acgctgtcga cggcgtacct
     7621 caacgcgctc gagttcacga agatccgcgt gcagggggcc gacctgctgc gcgccaacga
     7681 caagacggcg ccgcgcgtcc gcatcatcga gcacccggac gtgcgccgga tgttgatgct
     7741 gcaaaagtgc ttcgccgagg gcatgcgcgc gctgtgcctg ttcaccgcgt cgatccagga
     7801 cgaggtgacg ctcgcgggcg gccatggcag cgccgcggcg gccgagctcg accgcctcaa
     7861 cgacctgctg ttgccgctgg tcaagggcta ctgctccgag aaggtgtacg agctgctggc
     7921 gacctcgctg cagtgctacg gcggctcggg ctacgtgcag gactacccga tcgagcagta
     7981 catccgcgac cagaagatcg acacgctgta cgagggcacg acccacatcc aggcgctcga
     8041 cctgttcttc cgcaaggtcg cccgcgacgg cggcgagacc ctgcgcggcc tgatgggccg
     8101 cgtgatggcg acgctcgggg agatcgacgc caacggtccc ctggcggtcg agcacgcggc
     8161 gctgtcgcgt gcgctcggcg acctgcaggg catgtacgtg gcgatgctcg gcaagctcgg
     8221 cgagtcgatg taccacgtcg gtctgcaggg caaccgcgtg ctgttcgcca ccgccgaggt
     8281 cgtgatcggg tggttgctgg tgcgtcaggc cgcggtcgct gcggccgccc gcgagaccgc
     8341 ccgcgacggc gagcgcgcct tctacgacgg caagctggcg tcggtgcgtt tctacgcgcg
     8401 caacgtgctc ccggggatca cgctcgcccg caagctctgc gagcagagca cgctcgagct
     8461 catggaagtg cccgaagcgg cgttctgagg ccgtccaggc ggatctctgt cgccgctcca
     8521 cgcgcgggcg tagcatgccc gcgtcgtgga tgaagccctg tcgatcctgc gagccttgct
     8581 cgatgaacac gccgacgagg tcgtcgacga gggcaccgcg tgggtgcgcg ccgagtcgca
     8641 ggccgacctg tccgcgcggc cctgggccga gacccgcagc ctggtcgcgc agtgcctcgc
     8701 cttctatcgc gcccacctgt tcgacggcga caccgcgccg cgcgaggcgt tcatcgagct
     8761 ggtgacctcc aaccgctcga acctcaagtt ctcggtgtcg acgctgctgc gcggcttcat
     8821 gagctttcgc gtcgcggtgg tgcacctcgt cgcgcggatg gaggtccccc cggcgctggc
     8881 gctgacgatc acgcgcaagg tcgacgaggt gtcggcggag gcgtcgtttc gcaccgcgga
     8941 cctgtacgtc gccaagctca accgggtgat cgacgcgacc cgcgaggagc tgatccgcaa
     9001 ggacaagctg gccgcgctcg gcggcctggt cgccggcgtc gcccacgaga tcaacacgcc
     9061 gctcgggatc gccgtcaccg cgtcttcgct ggtcgccgat cgactccgcg atttatatga
     9121 ggccttcgag gccggcacgc tgcgccggca ggatctacaa aacgggctgg tccaggccca
     9181 ggaggccgcg cgcctgaccc tcggcaacct gcgccgcgcc gccgacatgg tcggcaactt
     9241 caagcagatc gccgtcgacc agaccagcga ggcgcgcc
//
LOCUS       DASWNJ010001900         1587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_138174, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001900 DASWNJ010000000
VERSION     DASWNJ010001900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138174"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VGB85_31695"
     CDS             complement(<1..222)
                     /locus_tag="VGB85_31695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138706.1"
                     /translation="MKPYPSISTKIRADVPIYAFDKLDGSQIRAEWTPKSGFGSFGSR
                     KRALERSHPWLGEAITLVRDKYEAAVGRVL"
     gene            complement(249..425)
                     /locus_tag="VGB85_31700"
     CDS             complement(249..425)
                     /locus_tag="VGB85_31700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138707.1"
                     /translation="MSVILGGAVDVAQARVPDGRPASRAMRAAWSASQARRIDVAATG
                     EGIAGFITHGQRAM"
     gene            671..1306
                     /locus_tag="VGB85_31705"
     CDS             671..1306
                     /locus_tag="VGB85_31705"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0138708.1"
                     /translation="MKVYGHPASTCTRKVLTTLAEKNHTADFVMIDIMKGEGHTPEYL
                     ARQPWGQVPVLEEDDGWQLFESRAIIRHLDATLPGPALTPANARDRARMEQWISIESA
                     NFTPGVMKILGQLLFAKWRGEEPKMTLVEEGRASVRKAVAVMEKTLAEHEYIAGKEFT
                     LADIGFMPYIEYLFVAGEGNLITDNPNTSAWWNRISERASWQQVRGKSSAA"
     gene            complement(1388..>1587)
                     /locus_tag="VGB85_31710"
     CDS             complement(1388..>1587)
                     /locus_tag="VGB85_31710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138709.1"
                     /translation="KLNTQKMCVAKPTFFECTDAVACDAVQTYACDAMVDPPEPFLFP
                     SSCLPMGWEPCEAPPVTMPCK"
BASE COUNT          287 a          549 c          504 g          247 t
ORIGIN      
        1 gagcacgcga cccaccgccg cctcgtattt gtcgcgcacc agcgtgatcg cctcgccgag
       61 ccacgggtgc gagcgctcga gcgcgcgctt gcggctgccg aagctgccga accccgactt
      121 cggggtccac tcggcgcgga tctgcgagcc gtcgagtttg tcaaaggcgt agatcggcac
      181 gtcggcgcgg atcttcgtgg aaatggatgg atatggcttc atgacgatct ccaggcgtaa
      241 cggatcgatc acatggctct ttggccatgt gtgatgaatc cggcgatgcc ctcgccggtc
      301 gccgcgacgt cgatgcgtcg cgcctgcgat gccgaccacg cggcccgcat cgcacgcgat
      361 gccggccgcc cgtcggggac gcgagcctgc gcgacatcca cggcgccccc aaggatcacg
      421 ctcatcccga gcgcatcgac ttgcgagcgg ctctcgcccg ctccctctca acaaagaaga
      481 gaacaggaga gccaggaccg gcggacgtcc gcgaggttca aaccatcatg accgggcgac
      541 gataaagcag gtccaccgcc ccgtcaagcg cgggtcggtc ggcttgttct cgcgcataat
      601 cttactcgct acgcccgatg tcaggacccg cggatgtcgt gataagagcc tgcgcggagg
      661 atcgtagcgc atgaaggtat atggacaccc cgcgagcacc tgcacccgca aggtgctgac
      721 caccctggcc gagaagaacc acaccgccga cttcgtgatg atcgacatca tgaagggcga
      781 gggccacacg cccgagtacc tggcgcgtca gccctggggt caggtccccg tgctcgagga
      841 ggacgacggc tggcagctgt tcgagtcgcg cgcgatcatc cgccacctcg acgccaccct
      901 gcccggcccc gcgctcacgc ccgcgaacgc gcgcgaccgg gcccggatgg agcagtggat
      961 cagcatcgag tcggccaact tcacccccgg cgtgatgaag atcctcggcc agctgctgtt
     1021 cgccaagtgg cgcggcgagg agcccaagat gacgctcgtc gaggagggcc gcgccagcgt
     1081 gcgcaaggcc gtcgccgtga tggagaagac cctcgccgag cacgagtaca tcgccggcaa
     1141 ggaattcacc ctcgccgaca tcggcttcat gccctacatc gagtacctgt tcgtcgccgg
     1201 tgagggcaac ctgatcaccg ataacccgaa caccagcgcc tggtggaacc gcatcagcga
     1261 gcgcgcctcc tggcagcagg tccgcggcaa gtcgtccgcc gcctgatctc ggcgtattcg
     1321 cgaacaggcg cccggcgatc accgggcgcc tgtttctttt taaattacat cgctcgtcgc
     1381 ggcgcgatca cttgcacggc atggtcacgg gcggggcctc gcacggctcc cagcccatcg
     1441 gcaagcacga gctcgggaac aggaacggct ccggcggatc gaccatcgcg tcgcaggcgt
     1501 aggtctgcac cgcgtcgcac gcgaccgcgt ccgtgcactc gaagaaggtc ggcttggcga
     1561 cgcacatctt ctgcgtgttg agcttgc
//
LOCUS       DASWNJ010001901         2551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_527612, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001901 DASWNJ010000000
VERSION     DASWNJ010001901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527612"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VGB85_31715"
     CDS             <1..204
                     /locus_tag="VGB85_31715"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0138710.1"
                     /translation="ILEDFNLEAAERRLCVEALALAGNIVGAARILGITRHALKRRII
                     KLHIDLARSRPSPDSDPAPSAEQ"
     gene            complement(336..722)
                     /locus_tag="VGB85_31720"
     CDS             complement(336..722)
                     /locus_tag="VGB85_31720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138711.1"
                     /translation="MLDIIDVSHSPRGDLAVVSESGATVAWPSGGGAPGIGRGGLHVR
                     STQAPEQVAHAAGRADCVTFTRDGERVAWSGMSGTIGRTFCATMTETGGQGRRTSPSA
                     MEVDAQLRPELLRCWGQRSRRGRLGG"
     gene            complement(1220..1441)
                     /locus_tag="VGB85_31725"
     CDS             complement(1220..1441)
                     /locus_tag="VGB85_31725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138712.1"
                     /translation="MTRTAKRRTAVRARADGVRIPLASYNLADAAACLAVEALKRGGS
                     IDEAAKLMRITQSRLQRIMDASGIKAPEP"
     gene            complement(1596..2402)
                     /locus_tag="VGB85_31730"
     CDS             complement(1596..2402)
                     /locus_tag="VGB85_31730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138713.1"
                     /translation="MDLGNQTSLTPDRDAQAEPGIQAPRPESGFYRTTSSVFYVTGDG
                     RVFLVSAPGEHVCLRPRDELAADAVRVVDITGAALLLVADTAEQLASPSGRHLRADAG
                     RDARVLAASLLQDLLVAASDTIGHVLLDEQPADVDALHERVGGLVLTSQAHRRRLSDQ
                     RVNSRGRSRGLPPPPPSIPRLALPFRHRGDYVGWFTGMLMAGEALGVASVRDAHLRGD
                     LWTVSQEGILHVFRCRAMTLADTGSGSPGLERPSQTVGTAELQPAAVSTP"
BASE COUNT          396 a          887 c          887 g          381 t
ORIGIN      
        1 atcctcgaag acttcaatct cgaagccgcc gagcggcggc tgtgcgtcga ggccctggcg
       61 ctcgccggca acatcgttgg cgcggctcgg atcctcggca tcacgcgcca tgccttgaag
      121 cgccgcatca tcaagctgca catcgacctg gcacgctcgc gcccgtctcc agactcggat
      181 ccggcgccct cggcagagca gtgacacgct ggagaatgcg ctgaacggcc gcgacgggag
      241 ctgtgctcga ggcgctccgg ctcgccgagg cggccttacc tgatcggctg ggggcctccg
      301 agcacccgcg taacggccga cgctgatggc ggtgctcacc cacccaggcg acctcgacga
      361 gaccgttgcc cccaacatcg tagtagctct gggcgcagct gggcatcgac ttccatggcc
      421 gagggtgatg tgcgccgtcc ctggccgccc gtctccgtca tcgtggcgca gaatgtcctg
      481 ccgatggttc ccgacatacc ggaccacgcc acgcgctcgc catcgcgagt gaaggtcacg
      541 cagtcggccc gcccggccgc gtgcgcgacc tgctccggcg cctgcgtgga ccgcacgtga
      601 aggccgccgc gaccgatgcc cggggcaccg ccgcccgacg gccacgcgac cgtcgcgccg
      661 ctctcgctga ccaccgcgag gtcgccgcgc ggcgagtggg acacgtcgat gatgtcgagc
      721 acgcggagct gcccgaggtc cccgcaacgt ctcgccgttg gcgaccgggc gctcgcgcag
      781 tcgcgtgcgg ccgccgaata cgaccgtgcg cccgtccggg gcgaagctcg cggtcgcgtg
      841 ctccagacac tcatcggcgg actcgcgcgg gctcgtccac ggtcggaaga aaggatgcgg
      901 gcgatgtcgt ctgggtctgt cccggcatcg aggaaacgca tgcggccgcc gcgcttgcgg
      961 cagacggtga cggcgacggc gaagacgcgg ccagcaggtt gcccagccga tgcggcgggg
     1021 tcgggtgcag ggctgcgagt cgtcatcgtc gaggccgaga gcgaattgct ggccgggggc
     1081 ggggacgaca aacggcggga gaacgcgagg gcggaggtgt ggctggcgaa cacgcgcgag
     1141 ctgcacgcag ccccccgacc tggaggcgtc gcagcccgtc agactctcgc ccacaggcgg
     1201 ccgagtcggg gttgtctcat caaggttcag gagccttgat gccgctcgcg tccatgatgc
     1261 gttgcaaccg gctctgggtg atcctcatca gcttggcggc ttcatcgatc gagccgccac
     1321 gcttgagcgc ctcgacggcg aggcacgcag cggcatcggc caagttgtag gacgccagcg
     1381 ggatcctcac cccatcggct ctcgcgcgga ccgcggtgcg gcgtttggcc gtgcgcgtca
     1441 ctgcaaactc ccggcgggcg ccgcgggttc gtcgtcgccc acggagacgt cttggccctc
     1501 gctgaaggcc cacgactcgc tgagcacctt attagatgca tcgccttcgt tgagtcggtc
     1561 ggcgacctca tcgcagagca agactgaacc gctcgtcatg gcgtgctcac cgctgctggt
     1621 tgaagctccg cagtgccgac ggtctgcgac ggtcgctcga gacccggcga tccgctgccc
     1681 gtgtccgcga gcgtcatggc gcggcaccgg aacacgtgga ggatgccctc ctgactgacc
     1741 gtccacagat cgccgcgcag gtgggcgtcg cgcacggaag ccacgccgag cgcctcacca
     1801 gccatgagca tgccggtgaa ccagccgacg taatcgcctc tgtgtcggaa cgggagcgcg
     1861 agccgcggga tcgacggcgg tggtggcgga agcccccgcg accgcccacg actgttgacg
     1921 cgctggtcgc tgaggcgtcg ccgatgcgcc tgcgaggtca gcaccagccc cccgactcgc
     1981 tcgtgcaagg catccacgtc ggccggctgc tcatcgagca gcacgtggcc gatggtgtcg
     2041 ctcgcagcga cgagcaagtc ctgcagcagg gacgccgcca gcacccgcgc gtcgcgacca
     2101 gcatccgcgc gcaggtgccg gccacttggt gaggccaact gctccgccgt atcagcgaca
     2161 agaagaagtg cagcaccggt gatatcgacg acccgtacgg cgtctgccgc gagctcatcg
     2221 cgcgggcgca ggcacacgtg ctcgccgggc gcgctcacga ggaacacgcg cccgtcgcca
     2281 gtcacgtaga agacggacga cgtggtcctg taaaagccgg actcagggcg cggggcctgg
     2341 atacctggct ctgcctgtgc gtcgcggtcg ggggtcagtg aggtttggtt gccgaggtcc
     2401 atcgatggtc acttcctggt cattgccgag tcattcggca acatcacgct aatcggcgtc
     2461 aacgagtcgc gcaagggcct gtcggccgtc caggcgacgc caggatggct ggcgcgcggg
     2521 tgcgcgctta ccctatactc cgcgggtcgt g
//
LOCUS       DASWNJ010001902         2781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_456051, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001902 DASWNJ010000000
VERSION     DASWNJ010001902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456051"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..1755
                     /locus_tag="VGB85_31735"
     CDS             118..1755
                     /locus_tag="VGB85_31735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138714.1"
                     /translation="MSMSGEVTGAESSSSSVPTSDASASETGAGSEGATTGPGTSTGE
                     PGGPPAPGGAWVLYLTNTYFQQGPSELFFVDCSGEVPAPPVRINEPLAPDTTIFFNVM
                     PDLSPRRRWLSYVVGGGAEGKELWFLRLDGPTMGPPRRVQLPPMRRFDRPEFGGPNEQ
                     MVAFRAQHDETSEAMWLCHLEEDGGCAPTQWSPLLADNGAPGGTHVAISPDGRWLAYT
                     GDPEGDEIDDMLLTRTEPGDAGSFVQVSAQLPDGFTWDPEFSAVSPTFYYTARDPEGV
                     EEELAVDLTSDTPGAPVVVAPTPYATRFLFNADRSAYLRNAGSDLELVTLAGTVGTPV
                     RLNAPEQVAGRYSTFVRQDQGVMFYVRKPDDLEENELFLVDISGPVPSAPLRLGEPLP
                     PGVEIEDYPVFGDDERQFFTVIDEAQGVETLWAAGTQDGAVVELTASLPPVEDISDFF
                     AIAEGHGRVAFTADGTGYHFDLAGAMTPVAFGPASSFSQGGGNFSADGQRLYYSHGTT
                     HGKAFLVDVGPSPGEPVQISDPAHGGLIEVIVVPPGG"
     gene            complement(1944..>2781)
                     /locus_tag="VGB85_31740"
     CDS             complement(1944..>2781)
                     /locus_tag="VGB85_31740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0138715.1"
                     /translation="QLAEVEAHHSDLYRRLPVMMHSIDARGRLCDVNDLWQETMGYPR
                     EEVLGRASVEFLTEQSREHAREVVLPEFFRTGRNLNAAYQMVRKDGRLLDVLLSAVAD
                     RDAEGKVVRSYAVIIDVTEMRRTEAAARRSQVQEEIIRAQEETLRALSTPLVPLGGGA
                     VLMPLIGAVDGSRAEQIVTALLAGVVAHTASKAILDVTGVPAFDAAVGAAIARATRAV
                     RLLGAEVVLTGLSPAAARTLVALAVDLGGVATRGTLRDGIAYALRDAGPSARSSHLAT
                     GP"
BASE COUNT          401 a          940 c         1028 g          412 t
ORIGIN      
        1 gacgccgcca cgacgccgtc gtcggcgaac gggctgcggt gaatcggata cgatgctggg
       61 atgcgctggc gtgcgagtgt gatggtgttg gtgtgcgtgg cgggctgcgg agacgacgtg
      121 agcatgtcgg gtgaggtcac cggcgcggag tcgtcgtcgt cgtcggtacc gacgagcgac
      181 gcttcggcgt cggagaccgg ggcgggcagc gagggcgcga cgacgggtcc tgggacgagc
      241 accggcgagc ccggcgggcc gccagcgccg ggcggggcgt gggtgctgta cttgaccaac
      301 acctacttcc aacagggccc gagcgagctg ttcttcgtcg actgcagcgg cgaggtgccc
      361 gcgccgccgg tccgaatcaa cgagccgctc gcgccggata cgacgatctt cttcaacgtg
      421 atgcccgacc tgtcgccacg gcggcgctgg ctcagctacg tggtcggcgg cggcgcggag
      481 ggcaaagagc tgtggttcct ccgcctcgac gggccgacga tggggccgcc gcgacgcgtg
      541 cagttgccgc cgatgcggcg gttcgatcgc ccggagttcg gcgggcccaa cgagcagatg
      601 gtggcgttcc gcgctcagca tgacgagacc agcgaggcga tgtggctgtg ccacctcgag
      661 gaagacggcg gctgtgcgcc gacgcagtgg agcccgctgt tggccgacaa cggcgcgccc
      721 gggggcaccc atgtggcgat ctcgccggat ggtcgctggc tggcgtatac cggtgatccc
      781 gaaggcgacg agatcgacga catgctgctc acccgcaccg agcctggaga cgccggctcg
      841 ttcgtgcagg tcagcgcgca gttgccggac ggcttcacgt gggacccgga gttctcggcg
      901 gtcagcccga cgttctacta cacggcccgt gaccccgagg gcgtggagga ggaacttgcg
      961 gtcgacctga cgagcgatac gccgggcgca ccggtggtcg tcgcgccgac gccgtacgcg
     1021 acacggttcc tgttcaacgc cgatcgctcg gcctacctgc gcaacgccgg gtccgacctc
     1081 gagctcgtca cgctggcggg cacggtcggc acacccgtgc ggctcaacgc gccggagcag
     1141 gtggccgggc gctacagcac gttcgtgcgg caagatcagg gcgtgatgtt ctacgtccgc
     1201 aagcccgacg acctcgagga gaatgagctg ttcctcgtcg acatctccgg gccggtgccg
     1261 tcggccccgc tgcgcctcgg cgaaccgctg ccgccgggcg tcgagatcga ggactacccc
     1321 gtgttcggcg acgacgagcg ccagttcttc accgtcatcg acgaggcgca gggcgtcgag
     1381 acgctgtggg cggccgggac gcaagacgga gcggtggtcg agctgacagc ctcactgccg
     1441 ccggtggagg acatctcgga cttcttcgcc atcgccgagg gacacggacg ggtcgcgttc
     1501 acggcggacg ggacgggcta ccacttcgac ctcgccggcg cgatgacgcc ggtggccttc
     1561 gggccggcgt cgtcgttctc gcaaggcggc ggcaacttct cggccgacgg acagcgcctc
     1621 tactactccc acgggaccac gcacgggaag gccttcctgg tcgacgtggg cccgtcgccg
     1681 ggcgaaccgg tccagatctc ggaccccgca cacggcgggc tgatcgaggt gatcgtcgtg
     1741 ccgccgggcg gttgacgagc agcccggctt cgcgttcgac ctcggcgggc gacgcatcgc
     1801 gctcgcgccc cacaccgccg gcgggtatga cttcgattgg tgcgtgctgc tcgagttgaa
     1861 tgcattgctc gcgcggagcc ggcgacggtt cgtctgcgcc cgcccgccgc gctcgacctc
     1921 gagggacaga taattatccg ccgtcaggga ccggtggcga ggtgtgacga cctcgcggag
     1981 gggcccgcgt cgcgcagcgc gtaggcgatg ccgtcgcgga gcgttccgcg ggtcgccaca
     2041 ccgccgagat cgaccgcgag ggcgaccaga gtgcgcgccg ctgcgggcga gagcccggtg
     2101 agcaccacct ccgcgccgag cagccgcacg gctctcgtgg cccgcgcgat cgcggcgccc
     2161 acggctgcgt cgaaggccgg cacgcctgtg acgtccagga tcgccttgga ggccgtgtgt
     2221 gcgaccacgc cagcgagcag cgccgtgacg atctgctcgg cgcgcgaccc gtccacggcg
     2281 ccgatgagcg gcatcaggac ggccccgccg ccgaggggca cgagcggcgt ggagagtgcg
     2341 cgcagcgtct cctcctgggc ccggatgatc tcctcctgca cctggctgcg ccgcgcagcc
     2401 gcctcggtgc gacgcatttc ggtgacgtcg atgatcaccg cgtaggagcg gaccaccttg
     2461 ccctcggcgt cgcggtcggc cacggcggag agcagcacgt cgaggagtcg tccgtcctta
     2521 cgaaccatct gatacgcggc gttgaggttc ctgccggtgc ggaagaattc agggagcacg
     2581 acctcgcggg cgtgctcccg ggactgctcg gtcagaaact cgacggaggc gcgcccgagc
     2641 acctcctcgc gagggtaccc catggtctct tgccagaggt cgttgacgtc gcagagccgt
     2701 ccgcgcgcgt cgatggagtg catcatcacc ggcagccggc ggtagaggtc gctgtggtgc
     2761 gcctcgacct ccgcgagctg g
//
LOCUS       DASWNJ010001903         4298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_669542, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001903 DASWNJ010000000
VERSION     DASWNJ010001903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669542"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2272)
                     /gene="cphA"
                     /locus_tag="VGB85_31745"
     CDS             complement(<1..2272)
                     /gene="cphA"
                     /locus_tag="VGB85_31745"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY0138716.1"
                     /translation="MQILERRTYRGPNVYAHFPVIRFTVDLGELEQHPSATIPGFVEG
                     LLAAIPSLQQHTCSYGDAGGFVRRLREDGGTWLGHVLEHVAIEVQVLTGAKVSFGKTR
                     GAGNPGQYLVVYEYEEERVGDAAGNLALDLLHSLLPADVPAFAVARAQVPADFDFRER
                     LDDLIAFAQKRQLGPSTGSLVRAAEKRDIPWIRLNDQSLVQFGHGRYQKRIQATITSE
                     TRYIAVGIASDKEETNKILGDLGLPVPRQQIVRNAEKAVAAAERLGFPVVVKPLDANH
                     GRGVSINLETAEAVRAAFEKAEEHSSSVIVENYIPGFDHRILVIGGRVIAVAKRVPGH
                     VVGDGERTVAQLIEQVNSDPRRGIGHEKVLTRIEIDYQARRLLELIGYTIETVPAAGE
                     VVYLRSTGNLSTGGTAVDLTDVIHPDNREMAIRAANAIGLDVGGIDFITPDISRSYRE
                     HGGGICEVNAAPGFRMHVAPTEGTPRDPAGPVMDMLFPPGTPTRIPIAAVTGTNGKTT
                     VARMVAHIHKMSGATVGLCTTDGVYIDGERTVTGDMTGPMAAQMILRDPSVDLAVLET
                     ARGGLVRAGMGYRACNVGAVLNITPDHLGLGGIHDLDQLAELKRIVVEVARDCAVLNA
                     DDERCLRMADYTEASRIGYVTMNARHDLVRKHIRAGGLAAVLEEGINGDMITIYDRGT
                     HIPLLWTHLIPATVEGKAMHNVQNAMFAVLMTYAMGVKVENIRQGLRTFDTTYFQAPG
                     RLNIYDELPFKVILDYG"
     gene            complement(2351..3343)
                     /locus_tag="VGB85_31750"
     CDS             complement(2351..3343)
                     /locus_tag="VGB85_31750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY0138717.1"
                     /translation="MKPLKLATYNINGVRKRLPILLEWLAKEAPDIVCLQELKATDAA
                     FPIAEIRAAGYGARWHGQQSWNGVAILARGDDPLERRRGLPGDPSDDQSRYIECAAHG
                     LLVGCLYLPNGNPQPGPKFDYKLAWFERLIKHAEPLCSGEYPVVLAGDYNVVPTDFDI
                     YDTKSWKKDALLQPESRECYRRLLGQGWTDSLRKIHPEQIFTFWDYFRQHWQRNAGLR
                     IDHLLLNPNLAPRLLDAGVDRWVRDLEGTSDHAPTWITLDMASATATPGEQITTKTAA
                     KKTATKKVSKAPAKTVSKAANKKTAKTATKAVAKKATGKTTGASAKKSASRKKT"
     gene            3567..>4298
                     /locus_tag="VGB85_31755"
     CDS             3567..>4298
                     /locus_tag="VGB85_31755"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0138718.1"
                     /translation="MARPPTREHDSLAATMASRGGRVPGDAMDGMTLAPAGTSGPVDP
                     LEKQLTRRALFPGRVHPIQIGRFTVLSLLGRGGMGVVYACYDDLLDRKVAVKVLHGEA
                     TGAHASARLLREAQALARLGHPNVVAVHDVGVVGERVYLAMEFVDGQTLGAWVEATRP
                     GWREVLRVILAAGEGLAAAHAKGLIHRDIKPDNIMVGGDGRVRVMDFGLAHGDGEANG
                     ARPALSGGVTHGHDAVSAELTRTGAV"
BASE COUNT          582 a         1311 c         1685 g          720 t
ORIGIN      
        1 ggccgtagtc gaggatgacc ttgaagggca gctcgtcgta gatgttgagg cggcccgggg
       61 cctggaagta ggtggtatcg aaggtgcgaa ggccctggcg gatgttctcg accttgacgc
      121 ccatcgcgta ggtcatcaaa accgcgaaca tggcgttttg cacgttgtgc atcgccttgc
      181 cctcgacggt ggcggggatc aggtgggtcc acagcagcgg gatgtgggtc ccgcggtcgt
      241 agatcgtgat catgtcgccg ttgatgccct cctcgagcac ggccgcgagg ccgccggcgc
      301 ggatgtgctt gcggacgagg tcgtgacggg cgttcatcgt cacgtagccg atgcgcgagg
      361 cctcggtgta gtcggccatt cgcaggcagc gctcgtcgtc ggcgttgagc acggcgcagt
      421 cgcgggcgac ctcgacgacg atgcgcttga gctcggcgag ctggtcgagg tcgtggatgc
      481 cgccgaggcc gaggtggtcg ggggtgatgt tgaggacggc gccgacgttg caggcgcggt
      541 agcccatgcc ggcgcgcacg aggccgccgc gggccgtctc gagcacggcg aggtcgacgg
      601 acgggtcgcg aaggatcatc tgcgcggcca tcggcccggt catgtcgccg gtgacggtcc
      661 gctcgccgtc gatgtagacg ccgtcggtgg tgcacagacc gacggtcgcg ccggacatct
      721 tgtggatgtg cgcgaccatg cgagcgacgg tggtcttgcc gttggtgccg gtgaccgcgg
      781 cgatcgggat gcgggtcggg gtgcccggcg ggaacagcat gtccatgacc ggaccggccg
      841 ggtcgcgcgg ggtgccctcg gtcggcgcga cgtgcatgcg aaagcccggc gcggcgttga
      901 cctcgcagat gccgccgccg tgctcgcggt acgagcggga gatgtcgggc gtgatgaagt
      961 cgatgccgcc gacgtcgagg ccgatcgcgt tcgcggcccg gatcgccatc tcgcggttgt
     1021 cggggtggat gacgtcggtg aggtcgacgg cggtgccgcc ggtgctcagg ttgccggtcg
     1081 agcggagata gacgacctcg ccggcggcgg gcacggtctc gatcgtataa ccgatcagct
     1141 cgagcaatct tcgggcctga tagtcgatct cgatgcgggt caggaccttc tcgtggccga
     1201 tgccgcggcg cgggtcgctg ttgacctgct cgatcagctg cgcgacggtg cgctcgccgt
     1261 cgccgacgac gtggccgggc acgcgcttgg cgacggcgat cacgcggccg ccgatgacga
     1321 ggatgcggtg gtcgaagccg gggatgtagt tctcgacgat gacggagctc gagtgctcct
     1381 ccgccttctc gaacgcggcg cgcacggcct cggcggtctc gaggttgatc gatacgccgc
     1441 ggccgtggtt ggcgtcgagc ggcttgacga cgacggggaa gccgaggcgc tcggcggcgg
     1501 cgacggcctt ctcggcgttg cggacgatct gctggcgcgg cacgggcagg ccgaggtcgc
     1561 cgaggatctt gttggtctcc tccttgtcgg aggcgatgcc gacggcgatg tagcgggtct
     1621 cagaggtgat cgtcgcctgg atccgcttct ggtagcgccc gtggccgaac tgcacgagcg
     1681 actggtcatt gaggcggatc cacgggatgt cgcgcttctc ggcggcgcgg accagcgagc
     1741 cggtcgaggg cccgagctgg cgcttttggg cgaaggcgat caggtcgtcg aggcgctcgc
     1801 ggaagtcgaa gtcggccggc acctgggcgc gcgcgacggc gaaggcgggc acgtccgcgg
     1861 gcagcaagga gtgcaggagg tcgagcgcga ggttgccggc cgcgtcgccg acgcgctcct
     1921 cctcgtactc gtagacgacg aggtactgcc cggggttgcc ggcgccgcgg gtcttgccga
     1981 acgagacctt ggcgccggtc aggacctgga cctcgatggc gacgtgctcg agcacgtggc
     2041 cgagccaggt gccgccgtcc tcacgcaggc ggcggacgaa cccgccggcg tcgccgtacg
     2101 agcaggtgtg ctgctggagg ctggggatcg cggcgagcag gccctcgacg aagcccggga
     2161 tcgtggcgga ggggtgctgc tcgagctcgc cgaggtcgac ggtgaagcgg atcaccggga
     2221 agtgggcgta gacgttggga ccgcggtaag tacgacgctc aaggatctgc attgcgaagt
     2281 cgggctcggt cggagccggg gacttttacc atgcggcccg ccagcgagcc gaccgaaacc
     2341 ttgcgtcggc tcacgtcttc ttgcgtgacg cggacttctt cgcggacgca ccggtggtct
     2401 tccccgtcgc ttttttggcg acggccttcg tggccgtttt ggcggtcttc ttgttggcag
     2461 ccttgctgac cgttttggcg ggcgccttgc tgaccttctt cgtggcggtc ttcttggcgg
     2521 cggtctttgt ggtgatctgc tcgccgggcg tcgcggtggc cgaggccatg tccagggtga
     2581 tccacgtcgg ggcgtggtcg ctggtcccct cgaggtcgcg gacccaccgg tcgacgccgg
     2641 cgtcgagcag gcgcggggcg aggttggggt tgagcagcag gtgatcgatg cgcaggccgg
     2701 cgttgcgctg ccagtgctgg cgaaagtagt cccagaaggt gaagatctgc tcggggtgga
     2761 tctttcgcag ggagtcggtc cagccctgcc cgagcaggcg ccggtaacac tcgcggctct
     2821 ccggctgcaa cagcgcgtcc ttcttccagg acttcgtatc gtagatatcg aagtcggtcg
     2881 gcacgacgtt gtaatcgccg gcgagcacga ccggatactc cccgctgcac agcggctcgg
     2941 cgtgtttaat taaacgctcg aaccacgcga gcttgtaatc gaacttgggg cccggctgcg
     3001 ggttaccgtt gggtagatac aaacagccga cgagcaggcc gtgcgcggcg cactcgatgt
     3061 agcggctctg gtcgtcggag gggtcgccag gcagaccgcg gcggcgctcg agcgggtcgt
     3121 cgccgcgggc gaggatcgcc acgccgttcc acgactgttg accgtgccag cgcgcgccgt
     3181 aaccagcggc gcggatctcc gcgatcggga aggcggcgtc ggtcgccttg agctcctgca
     3241 agcacacgat gtccggcgcc tcctttgcga gccactcgag caggatcggg aggcgcttgc
     3301 ggacgccgtt gatgttgtag gtcgcgagct tgagaggctt catgaggtcc cggacgggct
     3361 cgtacgcgcc cggcattgta gcgcgtgcgg ccctcgcagg cggctccccg gccggtatga
     3421 ccggacgccg gtcgtgggcg cgatcccgag cgtgggcagg cccggcgggt ctcgcggatc
     3481 gggctaggga gggcgtatac ggggcgctgg gcggggacgt gattttttct caattgatcg
     3541 gccgggccgg gacactgatc ctcgggatgg ctcgtccccc gacccgtgag cacgacagcc
     3601 tcgccgcgac gatggcctcg cggggcggtc gcgtgcccgg cgacgcgatg gacggcatga
     3661 cgctggcgcc ggccgggacg tcggggccgg tcgatccgct ggagaagcag ttgacccggc
     3721 gcgcgctgtt cccgggccgc gtgcacccga tccagatcgg gcgcttcacg gtgctgagcc
     3781 tgctcggccg cggcggaatg ggggtggtgt acgcgtgcta cgacgacctg ctcgaccgca
     3841 aggtcgcggt caaggtgctg cacggcgagg ccaccggcgc gcacgcgagc gcgcggctgc
     3901 tgcgtgaggc gcaggcgctg gcgcggctcg gtcatcccaa cgtggtcgcg gtgcacgacg
     3961 tcggggtggt cggcgagcgc gtgtatctgg cgatggagtt cgtcgacggg cagacgctcg
     4021 gcgcgtgggt ggaggcgacg cggccgggct ggcgcgaggt cctgcgggtg atcctcgcgg
     4081 ccggtgaagg gctggcggcg gcccacgcca aggggctcat ccatcgcgac atcaagcccg
     4141 acaacatcat ggtaggcggc gacgggcgcg tgcgcgtgat ggacttcggg ctcgcgcacg
     4201 gcgacggcga ggcgaacggg gcccggccgg cgctgtccgg gggcgtgacg cacggacacg
     4261 acgcggtgtc ggccgagctg acgcgcacgg gcgcggtg
//
LOCUS       DASWNJ010001904         5067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_317531, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001904 DASWNJ010000000
VERSION     DASWNJ010001904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317531"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="VGB85_31760"
     CDS             <1..959
                     /locus_tag="VGB85_31760"
                     /inference="COORDINATES: protein motif:HMM:NF026253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HEY0138719.1"
                     /translation="RFTMPFSSGLLPVRIEDKMGFVRPSGELVIPAAFLLTLGFSEGL
                     AAVKVAEGWGYIDPTGRVVIPPQFASASHFFDGLAHVHRGHHGGFIDKQGNFFADPPH
                     ASQVGRFAGGFAPYRALSRIGVPAWGYVDRHGAVALPPTYDIGGEFSEGLAAVQQDDL
                     YGYIDASFELVIPPQFTEAGEFHGGLAPVGVDGLTGYCDPSGAMVIPPAYTRGGAFAE
                     ELAPVFVGSRAGFIDRTGRMVIEPTFEAAQPFSEGLAAVRLQHRWGYVDRTGRRVVEP
                     IYESALAFSGGLACVRRFAEPKPVVHTGSKKVIVNLGGKPRS"
     gene            985..2103
                     /gene="rsgA"
                     /locus_tag="VGB85_31765"
     CDS             985..2103
                     /gene="rsgA"
                     /locus_tag="VGB85_31765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEY0138720.1"
                     /translation="MDDALLRLGLTPATAEAWTEHREAGLRLARVAAQHRGGYVVLSS
                     AAHVGIDIIEEAPTGIAELRADLSGRLRRAVAIDPADGPAVGDWVAVEVHGASATMHV
                     VLPRLSKLSRRAAGRGASEQLVAANVDTVFLVCPLDQPINLRRVERLLAISLESGAAA
                     VVVLTKADLCPDPAAAAATLGPHTDATGTPIPVHVISSVTGVGVEALDVYTHTPRTSA
                     LIGASGVGKSTLINRWLGHERQAVNAVREDGKGRHTTTHRELLVLPQGGLVIDTPGMR
                     ELGLWRSEEGLLGVFTDVVALAEQCRFRDCKHAEEPGCAIQAAVAAGTLPPERAASYL
                     KLAREQIHLDRERDVLARVRARQADKKVHRAARSHRKE"
     gene            complement(2123..3172)
                     /locus_tag="VGB85_31770"
     CDS             complement(2123..3172)
                     /locus_tag="VGB85_31770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138721.1"
                     /translation="MGCELTTLEHSPASMPVKVLTAPVLPPLLDPAVLATAVRAGVRQ
                     PDINQAVYEVDTFLVALTVEDLSSGRPSVTFEPVTEGVPGQRIRGVREGSVYAALGLL
                     DGDIIESINGVPLDGPGQALAGLGEGDRGVVVQVSRANVSAARELRFVGGLAWSHVLA
                     SRGGAPLPAEGPAVIADPVFPDMPSAPAPGAAPEPAGPAATTEPAGPPATTELAGPPA
                     TKRARAAGASPLAAAKSPAPTASSIECGLDGICSVARRDFDAVVADPDKLLRQVSVSE
                     ARGGYKLDQIRGGSQVSQLGFRNGDVIVSVNGVELDDSLGLLGLYGGLATTSSYKVVY
                     ERGGVRRGKVVRLRE"
     gene            3367..4206
                     /locus_tag="VGB85_31775"
     CDS             3367..4206
                     /locus_tag="VGB85_31775"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY0138722.1"
                     /translation="MDLFVDPRNTGPRRDDPRPRREGPPRPPEGGWAAWYLRDEDDVG
                     ESTEQGITVRTLLAALTELARERGWARTLVAGDQFFAWVESEPLVRISPDVYLLDDPP
                     APPYPAMWRTWLEGQAPPRFAVEVVSDDWAKDYVHAPRKYDQLGTHELIIFDPEAALP
                     RRGRRVVLQVFRRDAEGALVRVEAGEGPTHSAELDAWLVVQRGPAGARLRIARDASGT
                     DLVPTSEELVGALGARVFEYADAVTRKDSIIAEQDSIIARQEAIIAGLMAQLAGRTGE
                     PDE"
     gene            4361..>5067
                     /locus_tag="VGB85_31780"
     CDS             4361..>5067
                     /locus_tag="VGB85_31780"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0138723.1"
                     /translation="MAHHKLRRLRVYGGFLDKLDINFGDGLCCVIGGRGSGKTTVLEF
                     IRHALDRVPRSGSPSSKQAEERVRGLLAANLGATGCVELEFESKEGLVYSIRRTAHEP
                     PTVTDSNGKPVKPEMLCSSVLVDAAIFSHNQIEDIAHNPAAQRELIDRLASQPLQALE
                     ARLGQTTSLLRENAQRLVQLQVRIAGATRNLVDLSGWESKLSAAIAALGGEAGSAFKV
                     AADQKTVRDQERAALVRA"
BASE COUNT          711 a         1944 c         1775 g          637 t
ORIGIN      
        1 agcgcttcac catgccgttt agcagcggcc tcctgccggt gcgcatcgag gacaagatgg
       61 gcttcgtgcg cccgagcggc gagctggtga tcccggcggc cttcttgctg acgctcggct
      121 tctcggaggg cctcgccgcc gtcaaggtcg ccgagggctg gggctacatc gacccgaccg
      181 ggcgcgtggt gatcccgccg cagttcgcct ccgcgagcca cttcttcgac ggcctcgccc
      241 acgtgcaccg cggacaccac ggcggattca tcgacaagca gggcaacttc ttcgccgacc
      301 cgccgcacgc ctcgcaggtc ggccgcttcg ccggcggctt cgcgccgtac cgggcgttga
      361 gccgcatcgg cgtgccggcg tggggctacg tcgaccgcca cggcgcggtc gccctgcccc
      421 cgacctacga catcggcggc gagttctcgg agggcctcgc ggcggtccag caggacgacc
      481 tctacggcta catcgacgcc agcttcgagc tcgtgatccc gccgcagttc accgaggccg
      541 gcgagttcca cggcggcctg gcgccggtcg gcgtcgacgg cctgaccggc tactgcgacc
      601 ccagcggggc gatggtcatc ccgccggcct acacccgcgg cggcgcgttc gccgaggagc
      661 tcgcgccggt gttcgtcggc agccgcgccg gcttcatcga ccgcaccggg cgcatggtga
      721 tcgagccgac cttcgaggcg gcccagccgt ttagcgaggg cctcgccgcg gtgcgcctgc
      781 agcaccgctg gggctacgtc gaccgcaccg gtcgccgcgt cgtcgagccg atctacgaga
      841 gcgccctggc cttctccggc ggcctcgcct gcgtgcgccg cttcgccgag cccaagccgg
      901 tcgtgcacac cggctcgaag aaggtgatcg tcaacctcgg cggcaaaccc aggagctgac
      961 gaaatttcgg ctatggtgtg cgccatggac gacgcactcc tgcgcctcgg cctcacgccc
     1021 gcgaccgccg aggcgtggac ggagcaccgt gaggccggcc tccgactggc gagagtcgcc
     1081 gcccagcacc gcggcggcta cgtcgtgctc agcagcgcgg cgcacgtcgg gatcgatatc
     1141 atcgaggagg cgccgacggg catcgccgag ctgcgcgccg acctgtccgg acgcctgcgc
     1201 cgcgccgtgg cgatcgaccc cgccgacggc cccgcggtcg gcgactgggt cgcggtcgag
     1261 gtccacggcg cctcggcgac gatgcacgtg gtgttgccgc gcctgagcaa gctctcgcgc
     1321 cgggccgcgg ggcgcggggc gagcgagcag ctggtcgccg ccaacgtcga caccgtgttc
     1381 ctggtgtgcc cgctcgacca gccgatcaac ctccgccgcg tcgagcgcct gctggcgatc
     1441 agcctcgaga gcggcgcggc ggcggtggtc gtgctgacca aggccgacct gtgccccgac
     1501 ccggccgccg ccgcggcgac cctcggaccg cacaccgacg cgaccggcac gccgatcccc
     1561 gtgcacgtga tcagcagcgt caccggcgtc ggcgtcgagg cgctcgacgt gtacacgcac
     1621 acgccgcgca cctcggcgtt gatcggcgcg tccggcgtcg gcaagtcgac cctgatcaac
     1681 cgctggctcg gccacgagcg ccaggcggtc aacgcggtgc gcgaggacgg caagggccgg
     1741 cacacgacca cgcaccgcga gctgctcgtg ctgccgcagg gcggcctcgt catcgacacg
     1801 ccgggcatgc gcgagctcgg cctgtggcgc agcgaggagg gcctgctcgg ggtgttcacg
     1861 gacgtcgtcg cgctcgccga gcagtgccgg tttcgcgact gcaagcacgc cgaggagccc
     1921 ggctgcgcga tccaggcggc cgtcgccgcc ggcacgctgc cgcccgagcg cgcggccagc
     1981 tacctcaagc tcgcgcgcga gcagatccac ctcgaccgcg agcgcgatgt cctcgcccgc
     2041 gtgcgcgccc gccaggccga caagaaggtc caccgcgcgg cgcggtcgca ccgcaaggag
     2101 tgaggctgtc ccgtgggaca ggtcactcgc gcagccgcac gaccttgccc cggcgcactc
     2161 ccccgcgctc gtagacgacc ttgtagctgc tcgtggtcgc caggccgccg tacagcccga
     2221 gcaggccgag cgagtcgtcg agctcgacgc cgttgacgga gacgatgacg tcgccgttgc
     2281 gaaacccgag ctggctgacc tggctgccgc cgcggatctg gtcgagcttg tagccgccgc
     2341 gcgcctcgct gaccgagacc tggcgtaaca gtttgtccgg gtcggcgacc accgcgtcga
     2401 agtcacgccg ggcgaccgag cagatgccgt cgaggccgca ctcgatcgag ctggccgtcg
     2461 gcgcggggga cttcgcggcc gccagcgggc tcgcgccggc tgcacgcgcc cgcttcgtcg
     2521 ccggaggtcc ggcgagctcc gtggtcgccg gaggtccggc gggctccgtg gtcgccgcag
     2581 gtccggcggg ctccggggcc gcgcccgggg ctggcgcgga gggcatgtcc ggaaagacag
     2641 ggtcggcgat cacggccggc ccctcggcgg gcagcggcgc gccgccgcgg ctggcgagca
     2701 cgtgcgacca cgccaggccg ccgacgaagc gaagctcgcg ggccgcgctg acgttggcgc
     2761 ggctgacctg gacgacgacg ccgcggtcgc cctcgccgag ccccgcgagc gcctgcccgg
     2821 ggccgtcgag cgggacgccg ttgatcgact cgatgatgtc gccgtcgagc agcccgagcg
     2881 ccgcgtacac cgaaccctcg cgcacgccgc ggatcctctg ccccggtaca ccctcggtca
     2941 ccggctcgaa ggtgacgctc ggccggcccg acgagaggtc ctcgaccgtc agcgcgacca
     3001 aaaaagtgtc gacctcgtac accgcctggt tgatgtccgg ctggcggacc cccgcccgca
     3061 ccgccgtcgc gagcaccgca gggtcgagca gcggcggcaa gaccggcgcc gtcaggacct
     3121 tgaccggcat gctcgcgggc gagtgctcga gcgtcgtcag ctcgcagccc acgagggtac
     3181 ccgcgacgac acccgaaagg agccacgtac acgccgttcg cacaggcccg cagcgtagcg
     3241 cgaggcccag gccccgccaa gccgcccggg ccgccaggtt cggtccagcg acgatccagc
     3301 ctcgcagggt cgaccggcgc cgcgggcggc cggcgtgacg accgcggggc gtgctacgtt
     3361 gccgacatgg acctcttcgt cgatccgcgc aacaccggac cccgcaggga cgacccacga
     3421 ccccgcaggg agggcccgcc gcgacctccc gaggggggct gggccgcctg gtacctgcga
     3481 gacgaagacg acgtgggcga gagcaccgag caaggcatca ccgtacgcac gctgctggcg
     3541 gcgctgaccg agctcgcgcg cgagcgcggc tgggcccgca cgctggtcgc cggtgaccag
     3601 ttcttcgcct gggtcgagtc cgagccgctg gtccggatct ccccggacgt gtacctgctc
     3661 gacgacccgc ccgcgccgcc ctacccggcg atgtggcgca cctggctcga aggacaggcg
     3721 ccgccgcgct tcgccgtcga ggtcgtgtcc gacgactggg ccaaggacta cgtgcacgcg
     3781 ccgcgcaagt acgaccagct cggcacccac gagctgatca tcttcgaccc ggaggccgcc
     3841 ctcccccgcc gcgggcgccg tgtcgtcctc caggtgttcc gccgcgacgc cgagggcgcg
     3901 ctcgtccgcg tcgaggccgg cgagggcccg acccacagcg ccgagctcga cgcctggctc
     3961 gtcgtccagc gcggccccgc cggcgcccgc ctccgcatcg cccgcgacgc ctccggcacc
     4021 gacctcgtcc ccacctcgga ggagctcgtc ggcgcgctcg gggcccgggt gttcgagtac
     4081 gcggacgccg tcacccgcaa ggactcgatc atcgccgagc aggactcgat catcgccagg
     4141 caggaggcca tcatcgccgg gctcatggcc cagctcgccg gccgtaccgg cgagccggac
     4201 gagtagcccc cgcctggccc aaagggcaga cgggccggcc gaccggccgc caaactgcct
     4261 tatctgaacc agaacaagcg cttcctggcc cctggacgga tccgcgcgcg cgacttaagg
     4321 tgatggaagg cggccctcca cggtccgccg gaggttcccc ttggctcacc ataagctccg
     4381 acgtctccgc gtatacggcg gctttctcga caagctcgac atcaacttcg gcgacggcct
     4441 gtgctgcgtc atcggcggcc gcggcagcgg caagacgacc gtcctcgagt tcatccgcca
     4501 cgccctcgac cgcgtgccgc ggtccggcag cccgtcctcg aagcaggccg aggagcgcgt
     4561 gcgcgggctc ctggccgcca acctcggcgc gaccggctgc gtcgagctcg agttcgagag
     4621 caaggagggc ctggtctaca gcatccgccg caccgcccac gagccgccga cggtcaccga
     4681 tagcaacggc aagccggtca agccggagat gctctgctcg agcgtgctcg tcgacgccgc
     4741 gatcttcagc cacaaccaga tcgaggacat cgcccacaac ccggccgccc agcgcgagct
     4801 gatcgaccgc ctggcgagcc agccgctgca ggcgctggag gcccgcctcg gccagaccac
     4861 ctcgctgctg cgggagaacg cccagcgcct cgtgcagctg caagtgcgga tcgcgggcgc
     4921 gacgcgcaac ctcgtcgacc tgtccggctg ggagagcaag ctgtccgccg cgatcgcggc
     4981 gctcggcggc gaggccggct cggcgttcaa ggtcgccgcc gatcaaaaga ccgtgcgcga
     5041 ccaggagcgg gccgccctcg tgcgcgc
//
LOCUS       DASWNJ010001905         2865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_317543, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001905 DASWNJ010000000
VERSION     DASWNJ010001905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317543"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VGB85_31785"
     CDS             complement(<1..855)
                     /locus_tag="VGB85_31785"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY0138724.1"
                     /translation="MTGHRSLRHCVEDLERHGHLRRIEVEIDARLEAAEIQRRVYQAG
                     GPALLFTRVKDCRFPMVSNLFGSMERMRFMFRGTLAAVRKLVELRTDPGALARRPFRY
                     LGVPFTALRMLPKFRRRGPVLAHETTISALPQMISWPGDGGAFITLPQVYSEDPDQPG
                     FARSNLGMYRVQLSGGQYAPDRQIGLHYQIHRGIGVHHAAAIRRGERLRVNIFVGGPP
                     ALTFAAVMPLPEGLPELAFAGALGGRRVRMIRRGGELPFAGDADFVITGTIDPDLRLP
                     EGPFGDHLG"
     gene            complement(1003..1923)
                     /locus_tag="VGB85_31790"
     CDS             complement(1003..1923)
                     /locus_tag="VGB85_31790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138725.1"
                     /translation="MTRLKLSLCLTSLALISSLTACSDRKPGSDAGEGSSSSSGDGET
                     TIEPTPTTSTDPTTPVTTQQDSEGTATEGATGTTTGNTSVTTGEPSCMDPIEGAPDNT
                     PCTDASGCGCESGKCFLVPILGGWCGECLGDVDCSPGGCTVPNPIDGVGATCNMGEPG
                     AGCESSDVCTDPANANCGTLLEVMGIIKVATCGECATNADCTTDPALTNCAPTYDVMN
                     FSGRYVCVGDNTTPNSEGCNLTDDGQGEPVGNKACMSGFCGEANVMGLLKVGICGECN
                     SNADCPMDKPTCTDPQVDLNNAALVGSVCM"
BASE COUNT          421 a         1030 c          980 g          434 t
ORIGIN      
        1 gccgaggtgg tcgccgaacg gcccctccgg cagccgcagg tccgggtcga tcgtgccggt
       61 gatcacgaag tccgcgtccc ccgcgaacgg cagctcaccg ccgcgacgga tcatgcggac
      121 ccgtcgcccc ccgagcgccc ccgcgaacgc gagctcgggc agaccctccg gcaggggcat
      181 caccgccgcg aaggtcagcg ccggcggacc gccgacgaag atgttgacgc gcagccgctc
      241 gccacgacgg atcgccgccg cgtgatgcac accgatgccg cggtggatct gataatgcag
      301 gccgatctgg cggtccggtg cgtattgccc gcccgacaat tgcacgcggt acatcccgag
      361 gttggaccgc gcgaagccgg gctggtcggg gtcctcgctg tagacctgcg gcagcgtgat
      421 gaacgctccc ccgtcgcccg gccaggagat catctgcggc agcgccgaga tcgtcgtctc
      481 gtgcgcgagc accggcccgc gccgacgaaa cttcggcaac atgcgcagcg ccgtgaacgg
      541 caccccgagg tagcgaaacg gccgtcgcgc cagcgccccg gggtccgtgc gcagctcgac
      601 gagcttgcgc accgccgcga gcgtcccgcg gaacatgaag cgcatgcgct ccatgctgcc
      661 gaacaggttg ctgaccatcg ggaaccggca gtccttgacg cgcgtgaaca gcagcgcggg
      721 cccgccggcc tggtacacgc gccgctggat ctccgcggcc tcgagcctgg cgtcgatctc
      781 gacctcgatc cggcgcaggt gcccgtgacg ctcgaggtcc tcgacgcagt ggcggagaga
      841 gcgatggccg gtcatgcgcg ggccgagcat acccgcggac ccccacccgc gcccgcccct
      901 gcgacacgtg agctgacggc caaagacaca gacggcgccc gtgacacgga cgccgtctcg
      961 ccacgggccc agggcccgct cacatgatca acacgaagca gttcacatgc acacggagcc
     1021 gacgagcgcc gcgttgttga ggtcgacctg cgggtcggtg caggtcggct tgtccatcgg
     1081 gcagtcggcg ttgctgttgc actcgccgca gatgccgacc ttgagcagac ccatcacgtt
     1141 cgcctcgccg cagaagcccg acatgcaggc cttgttgccg accggctcgc cctggccgtc
     1201 gtccgtcagg ttgcaaccct cgctgttggg cgtggtgttg tcgccgacgc acacgtagcg
     1261 gccgctgaag ttcatgacgt cgtaggtcgg cgcgcagttg gtcagcgccg ggtcggtcgt
     1321 gcagtccgcg ttcgtggcgc actcgccaca ggtcgcgacc ttgatgatgc ccatgacctc
     1381 gagcagcgtc ccgcagttgg cgttggccgg gtcggtgcag acatcgctgc tctcgcagcc
     1441 cgcgccgggc tcgcccatgt tgcaggtcgc gccgacgccg tcgatcgggt tcggaaccgt
     1501 gcagccgccc ggcgagcagt cgacgtcgcc cagacactcg ccgcaccagc cgccgaggat
     1561 cgggaccagg aagcacttgc cggactcaca gccgcagccc gaggcgtccg tgcacggcgt
     1621 gttgtccggc gcgccctcta tcgggtccat gcaggacggc tcgccggtcg tcacgctggt
     1681 attcccggtc gtcgtcccgg tcgcaccctc cgtggccgtc ccctcgctgt cctgctgcgt
     1741 cgtcaccggg gtcgtcgggt cggtgctggt cgtcggggtc ggctcgatgg tggtctcacc
     1801 gtcgcccgag ctggacgagc tgccctcgcc ggcgtcgctg ccaggcttgc ggtcgctaca
     1861 ggcggtcagc gaggagatga gggcgagaga agtaagacag agggaaagct tgaggcgcgt
     1921 catcggggca ttctccgggc gaaaagatca ggtcaggcag ggtcagaggg gggtgtcgcg
     1981 gccggcgccg caaacgaggg cgctggtgac cgcggtcgcc aggagagccc gtcgaacgag
     2041 atggaacgag gatgaagtag tacagccgga tcctggcgac atgcaagtga gacgcgaaac
     2101 cgggcgtctt gctcctgcaa gctcgcttgg ctaaagtcgt gagcttgcat gggtggccga
     2161 gcactcgcct cggagtccgg ttcctctgcc gctggagatc ttcaacagcc cgcactccgc
     2221 acgttcatga cgcacctgct cgcccgctcg ctcgcttgcc cgtctcgccc gggggaactc
     2281 gtcgcccgtt cgccgcacgc ggggcggccg ggggagctcg tcggccgttc gcagcacatg
     2341 gggcgtccgg gtccactcgt cgcccgttcg cagcacgtgg ggcggccggg tccactcgtc
     2401 gcccgttccg agcacgcggg gcgacgctat ccgggccggg agggaggggc cgacggacgg
     2461 cgggtccctg cgacgcgggt cctcccaggg gacgtgctcg ccgccccatc tgtcccgaag
     2521 gtcatcttcg tttcaccgag catgtcgcgc gcgaccggga ggacgagggg gcgtctgcgc
     2581 gcgacccctg ggtcctcccg ctgcttcccc cgctcgtcgg cccctgcctc ccggaccacc
     2641 accgtctgcg ccccgcttgt ctccggccgt gagatcgtcg cccgagcgat acaccccgtc
     2701 accaccgcag cgcctcgcca cgacaccaac aatgttcgag ggctcgtggc cacggctgcc
     2761 cttgtccaag gtcgcacccg cgcaatcggt gaatcccgcg tgcatccgga tgaacccgct
     2821 gcgacgacca tgtattccgg cccccgccgc aatgctggca cgtcg
//
LOCUS       DASWNJ010001906         6471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_317554, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001906 DASWNJ010000000
VERSION     DASWNJ010001906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6471
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317554"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1971)
                     /locus_tag="VGB85_31795"
     CDS             complement(<1..1971)
                     /locus_tag="VGB85_31795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138726.1"
                     /translation="MGATTDSSSSSGASTSSEPTTSTTSTTDVPTGTDATETTPTTDP
                     ITTSTTDPITSSTTETTETTDTSGTSDTTSTTDTTDAGTSSTTSSAACGDGFVDDGEQ
                     CDDGNALNDDACLDTCVLATCGDGEQQLDVEQCDDGNADNLDGCTASCLSAVCSPPVA
                     TRGSALAISPDDGRLVVANRENGTVSVLSVGYEGDLPTMTVIDELAVGGEPWQVGLDR
                     CGTTAYAILRRDQSLVEIQDILGVPTLGKTVAVGSEPTGLALTPNGTRIYVANWVDGT
                     LTVVDPLTMTQTGVVDLNAALANHPNKFLGDVAARPALAHPRSLAITSDGDADDTDEK
                     VYVTEYFAQRSAPEANNGLNGDINWVGVVYSVKVSDGSVKVVQLPPVLDTGFAAPNMQ
                     PTGCFPNQLQAISLSGKFAYVSSICASPVGPIGGKVNTHPLVTVIDTSNDAIVLDGTA
                     NLDRNFDLRYAMKATPDTSARRFPHAPADIGFDPTTGDAYIAANGADALFRAIYSPNT
                     GALTAIGKNGAPDFVDLAPAALKMLGKAGQNPVGVVVAAAHPFAFVANDVTMNVSAID
                     LEQQIVAGSDADDPRVVQSSPLPVDPDLQSALRGKHFFNTGLGRWSLNGQAWGSCQAC
                     HGDGLSDNVTWYFAKGPRQSTSLEGSFSKKDPT"
     gene            2466..3743
                     /locus_tag="VGB85_31800"
     CDS             2466..3743
                     /locus_tag="VGB85_31800"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEY0138727.1"
                     /translation="MPRCAADPTTMYVHWQDLDLLDEGILSWNCCYLDPATGRFTANS
                     SGHPLLAGVQPQALHSMGRDEGSLDPSRDTLLRTRYVMLELRERRACANGLADPALTT
                     RLLRILDEPGSPEQRAALTAYIAAKKRPRDQRELGELVERVAAEDPEGALSWCDDHEF
                     ALLRIAAFDRALRSADPEARSRLSESLHQWNRSDLWAWSQRLPDPAGNPPPWLPEVLA
                     WRAQQAEARRVRVAGLLARLDAWLAGHAPAVHAQLRPGADAGVLDACEQALGRPLPPT
                     LRQLWGWHDGGGELMPVAFVYSLRSVEEMLAMRAQRHGWWADAPGWWGPGWLPILEDP
                     SGNVIAVDLDGDFTGTPAQMIGFWHDSEDRDIVAHDLESMLEILVSIHESGAIGTDEH
                     GLDGVSQWDWKMAARRLTGTRGAVFRATPPRDR"
     gene            complement(3744..4712)
                     /locus_tag="VGB85_31805"
     CDS             complement(3744..4712)
                     /locus_tag="VGB85_31805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138728.1"
                     /translation="MPRWMPRRRAPPPVMLPERRVELDAIDEVHELLATAPPEDLLHG
                     RTPVTAPDAAIVQTALGRRWYAMVESTHGRLVRGRAYLREGAVLDLQVQRGLLTAEVQ
                     GTSRYRVKIAVSLPVGEQVTDLRDRLASLAGRTGADLHDAILPFADAIVPAPRHIVPF
                     CSCMDSTPFCKHVCAALHGFGVRLDGQPELLLLLRGLLPDASAPPGPLLAPLAPEKRL
                     TGDLTEIFGIDIVASTSPGAAESPQPGAPDLRDAAAPSSLPEVTREYLRTVGVPTHFI
                     DAWLREGVLRRTHRHGIYERTAEADRRIAALMPGDDGTGSSDPANP"
     gene            complement(4719..>6471)
                     /locus_tag="VGB85_31810"
     CDS             complement(4719..>6471)
                     /locus_tag="VGB85_31810"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HEY0138729.1"
                     /translation="DDELTADEWRELMQGEAGLHRLRGRWVELEPARHAEALRHWQGA
                     EAAAAKGALGFAEGMRLLAGLPRDSLAEATPVRWTVARPGPWLASLLAALQNPSGNAE
                     ADPGPALHGVLRPYQRDGVAWLWLLTRLGLGGCLADDMGLGKTVQVIALLLLMQQRGV
                     AGPHLIVLPASLLGNWRAELDRFSPGLRVHVAHRGAGEVDSAPDFTRIDVVLTTYGTL
                     LRQEWLQAQAWGLVALDEAQAIKNPQAKQTRAVKALRSRHRLVLTGTPVENALVDLWS
                     LFDFSSPGLLGGLTEFKKFSKRLGNDRSGMEPLRTLVRPYILRRLKTDRSVIADLPDK
                     TELQVYCGLTRVQAALYAKAVESLAREVASTDGIRRRGIILAYLTRFKQICNHPSQLS
                     RDGKYRASDSAKFLRLQELCAPIAEAGEKLLVFTQFREICDPLVELLTGCFSRPGLVL
                     HGGTPVAKRGEIVADFQRDAGPPFMVLSLRAGGTGLNLTAAAHVVHFDRWWNPAVENQ
                     ATDRAYRIGQHKNVLVHKFVCRGTLEERIDAMLHSKQHVADGILEDDGERRLTELATD
                     ELMRVVALDLRSALSED"
BASE COUNT          903 a         2093 c         2522 g          953 t
ORIGIN      
        1 ggtcgggtcc ttcttggaga acgagccctc gaggctggtc gactgccggg gccccttggc
       61 aaaataccag gtgacgttgt cggacagacc gtcgccgtgg cacgcctggc acgagcccca
      121 ggcctgaccg ttgagcgacc agcggccgag gccggtgttg aagaagtgct tgccgcgcag
      181 ggccgactgg aggtccgggt cgacgggcaa ggggctcgac tggacgacgc gcgggtcgtc
      241 ggcgtcgctg ccggcgacga tctgctgctc gaggtcgatc gccgagacgt tcatggtgac
      301 gtcgttggcg acgaaggcga aggggtgagc ggcggcgacg acgacaccga ccgggttctg
      361 gccggctttg ccgagcatct tgagggccgc gggcgcgagg tcgacgaagt cgggcgcgcc
      421 gttcttgccg atcgccgtca gggcgccggt gttcgggctg tagatcgcgc ggaacagggc
      481 gtcggcgccg ttggccgcga tgtaggcgtc gccggtggtc gggtcgaagc cgatgtcggc
      541 gggcgcgtgc gggaaccggc gggcgctggt gtccggcgtc gccttcatcg cgtagcgcag
      601 gtcgaagttg cggtcgaggt tggcggtgcc atcgagcacg atcgcgtcgt tgctcgtgtc
      661 gatgacggtg acgagcgggt gggtgttgac cttgccgccg atcgggccga ccggcgaggc
      721 gcagatcgaa gagacgtacg cgaacttgcc ggagaggctg atcgcctgga gctggttcgg
      781 gaagcagccg gtcggctgca tgttgggcgc ggcgaagccg gtgtcgagca ccggcgggag
      841 ctgcacgacc ttgaccgagc cgtcggagac cttgacgctg tacacgacgc cgacccagtt
      901 gatgtcgccg ttgaggccgt tgttggcctc cggagcgctg cgctgggcga aatactcggt
      961 gacgtagacc ttctcgtcgg tgtcgtcggc gtcgccgtcg ctggtgatcg cgagcgagcg
     1021 cggatgggcg agcgcggggc gggcggcgac atccccgagg aacttgttgg ggtggttggc
     1081 gagcgcggcg ttgaggtcga cgacgccggt ctgcgtcatc gtcagcgggt cgacgaccgt
     1141 gagggtgcca tcgacccagt tggcgacgta gatgcgcgtg ccgttgggcg tcagcgcgag
     1201 gccggtcggc tccgagccga cggcgacggt cttgccgagc gtcggaacgc cgaggatgtc
     1261 ctggatctcg accagcgact gatcgcgccg gaggatcgcg tacgcggtgg tgccacagcg
     1321 atcgaggccg acctgccagg gctcgccgcc gaccgcgagc tcgtcgatca cggtcatcgt
     1381 cggcaggtcg ccctcgtagc cgaccgagag caccgagacg gtcccgttct cgcggttggc
     1441 gacgaccaga cgcccgtcgt ccggcgagat cgccagcgcc gagccgcgcg tcgcgaccgg
     1501 cggcgaacag accgccgaca ggcagctcgc cgtgcagccg tcgaggttgt cggcgttgcc
     1561 gtcatcacac tgctcgacgt cgagctgctg ctcgccgtcg ccgcaggtgg cgagcacgca
     1621 ggtgtcgagg cacgcgtcat cgttgagcgc attgccgtcg tcacactgct ccccgtcgtc
     1681 gacgaagccg tcgccgcagg ccgccgagct ggtggtcgag gaggtgcccg cgtcggtggt
     1741 gtcggtggtg ctggtggtgt cggaggtacc cgaggtgtcc gtggtctcgg tcgtctcggt
     1801 cgtcgaggag gtgatcgggt cggtcgtcga cgtggtgatc gggtcggtcg tcggggtggt
     1861 ctcggtcgcg tcggtgccgg tcgggacgtc cgtggtggag gtggtggagg tggtgggctc
     1921 cgaggacgtg ctcgcgcccg aggacgagga gctgtcggtc gtggcgccca tgccgtcgtc
     1981 tccgcacgcg atcggagcga cgccgcaaag caggacggcg atcgcaccga gggcgccagg
     2041 gcccggtcga tcgctgtcgg ccgtggactg cctggcggga cgggtgcggg tcggatcgag
     2101 tctggtaggt tcttgcatga cggcttctct gcgctcgccg ggcgcgacaa ttggtggcaa
     2161 tgtggccaca tccgcgtatc gtgggccgat acgaggtccc cgaatgtggc aaaacaacga
     2221 cgattacgcg cgccgcgtga aatccacgag acagcggcac cggatattag gcgaacaatt
     2281 gaaattaaga catcggtcgt tcgctgctat gcgatcgcgg ccgaacatcg cctggcgcgg
     2341 gttggcaggc cgtgcgaaaa gggccggcgg atcggcgagc gatacagagc ccgcgacacg
     2401 atcgatcgcg gcaagccagg ctccgcgaga tagaagatga gccgggatga ctcgccgcgg
     2461 cgcccatgcc acgatgcgcc gcggacccga cgacgatgta cgtgcactgg caagatctcg
     2521 atctgctcga cgagggcatc ctgtcgtgga actgttgtta cctcgatccg gcgaccgggc
     2581 ggttcacggc gaacagcagc ggccacccgc tgctcgcagg ggtgcagccg caggcgctgc
     2641 attcgatggg gcgcgacgag ggctcgctcg acccgtcgcg cgacacgctg ctgcgcaccc
     2701 ggtacgtgat gctcgagctc cgcgagcgcc gcgcctgcgc gaacggcctc gccgacccgg
     2761 cgctgacgac ccggctgctg cggatcctcg acgagcccgg ctccccggag cagcgcgccg
     2821 cgctcacggc gtacatcgcg gcgaagaagc ggccacgcga ccagcgcgag ctcggggagc
     2881 tcgtcgagcg cgtcgcggcc gaggaccccg agggcgcgct gtcgtggtgc gacgaccatg
     2941 aattcgccct gctgcggatc gccgcgttcg accgcgcgct gcgttcggcg gacccggagg
     3001 cgaggtcgcg gctgtcggag tcgctgcatc agtggaaccg cagcgacctg tgggcgtggt
     3061 cgcagcggct gcccgacccg gccggcaatc cgccgccgtg gctgcccgag gtgctcgcct
     3121 ggcgcgcgca gcaggccgag gcccggcgcg tgcgcgtcgc ggggctgctc gcgcgcctcg
     3181 acgcctggct cgccggccac gcgccggccg tgcacgcgca gctgcgaccg ggcgccgacg
     3241 cgggtgtgct cgacgcgtgc gagcaggcgc tcgggcgccc gctgccgccg acgctgcgcc
     3301 agctgtgggg ctggcacgac ggcggcggcg agctgatgcc ggtcgcgttc gtgtactcgc
     3361 tgcgatcggt cgaggagatg ctcgccatgc gtgcgcagcg gcacgggtgg tgggccgacg
     3421 cgccgggctg gtggggaccg ggctggctgc cgatcctcga ggacccgtcg ggcaacgtga
     3481 tcgccgtcga cctcgacggt gatttcaccg gcacgccggc ccagatgatc ggcttttggc
     3541 acgacagcga ggaccgcgac atcgtcgccc acgacctcga gtcgatgctc gagatcctgg
     3601 tgtcgatcca cgagtccggc gcgatcggga ccgacgagca cgggctcgac ggtgtcagtc
     3661 agtgggactg gaagatggcc gcgcgccggc tcaccggcac ccgtggcgcg gtgttccgcg
     3721 cgacgccccc gcgcgaccgc tgattacgga tttgctggat ccgacgagcc cgtgccgtcg
     3781 tcgccgggca tcagcgcggc gatcctgcga tcggcctcgg cggtgcgctc gtagatgccg
     3841 tggcggtggg tgcgacgcag cacgccctcg cgcagccagg cgtcgatgaa gtgggtcggc
     3901 acgccgacgg tgcgcagata ctcgcgggtg acctcgggca ggctcgatgg ggccgcggcg
     3961 tcgcgtaaat cgggagcacc cggctgcggc gactcggcgg cgccggggct ggtcgaggcg
     4021 acgatgtcga tgccgaagat ctcggtgagg tcgccggtga ggcgcttctc gggcgcgagc
     4081 ggggccagca gcgggccggg cggggccgag gcgtcgggca gcagaccgcg cagcagcagc
     4141 aacagctccg gctggccgtc gaggcgcacg ccgaagccgt gcagcgcggc gcagacgtgc
     4201 ttgcaaaacg gggtgctgtc catgcatgag caaaacggga cgatgtggcg cggcgccggg
     4261 acgatcgcgt cggcgaacgg aaggatcgcg tcgtgcaggt cggcgcccgt tcggccggcg
     4321 aggctcgcca ggcggtcgcg caggtcggtg acctgctcgc cgaccggcag cgacacggcg
     4381 atcttgacgc gatagcggct ggtgccctgg acctccgccg tcaacagccc gcgctggacc
     4441 tgcaggtcga gcacggcccc ctcgcgcagg taggcgcggc cgcgcacgag ccggccgtgg
     4501 gtcgactcga ccatcgcgta ccagcgccgc cccagcgccg tctgtacaat cgccgcgtcg
     4561 ggggcggtca ccggcgtacg accgtgcagc aggtcctcgg gcggcgcggt ggccagcagc
     4621 tcgtggacct cgtcgatcgc gtcgagctcg acccgacgct cggggagcat cacaggcggc
     4681 ggcgcgcggc ggcggggcat ccagcggggc atcggcggtc aatcctcgct gagggcgctg
     4741 cgcaggtcga gggcgacgac gcgcatcagc tcgtcggtgg cgagctcggt gaggcggcgc
     4801 tcgccgtcgt cctcgaggat gccgtcggcg acgtgttgtt tgctgtgcaa catggcgtcg
     4861 atgcgctcct cgagggtgcc gcggcacacg aacttgtgga cgagcacgtt cttgtgttgg
     4921 ccgatgcgat aggcgcggtc ggtcgcctgg ttctcgacgg cggggttcca ccagcggtcg
     4981 aagtggacga cgtgggcggc cgcggtgagg ttgaggccgg tgccgccagc gcgcagcgag
     5041 aggaccatga agggtggtcc ggcgtcgcgt tgaaagtcgg cgacgatctc gccgcgcttg
     5101 gcgaccggcg tgccgccgtg cagcacgagg ccggggcgac tgaagcagcc ggtcagcagc
     5161 tcgacgagcg ggtcgcagat ctcgcgaaac tgcgtgaaca ccagcagctt ctcgccggcc
     5221 tcggcgatcg gggcgcacag ctcttgtaag cgcaagaact tggcggagtc cgaggcgcga
     5281 tacttgccgt cgcggctcag ctgcgagggg tggttgcaga tctgcttgaa gcgggtcagg
     5341 tacgcgagga tgatgccgcg gcggcggatg ccgtcggtcg aggcgacctc gcgcgcgagc
     5401 gactcgacgg ccttggcgta cagcgccgcc tgcacgcgcg tcagcccgca gtagacctgc
     5461 agctcggtct tgtcgggcag gtcggcgatc acgctgcggt cggtcttgag gcggcgcagg
     5521 atgtacgggc gcaccagcgt gcgcagcggc tccatgccgg agcggtcgtt gccgaggcgc
     5581 ttgctgaact tcttgaactc ggtgaggccg ccgagcaggc cggggctcga gaagtcgaac
     5641 agcgaccaga ggtcgacgag cgcgttctcg acgggggtgc cggtgagcac caggcgatgg
     5701 cggctgcgca gcgccttgac ggcgcgcgtc tgcttggcct gcgggttctt gatcgcctgg
     5761 gcctcgtcga gcgcgacgag gccccacgcc tgggcctgca gccactcctg gcgcaggagg
     5821 gtgccgtagg tggtcagcac gacgtcgatg cgcgtgaagt cgggcgcgct gtcgacctcg
     5881 ccggcgccgc gatgcgcgac gtgcacgcgc aggccggggg agaagcggtc gagctcggcg
     5941 cgccagttgc cgagcagcga ggccggcagg acgatcaggt gcgggccggc gacgccgcgc
     6001 tgctgcatca gcaacaacag cgcgatcacc tgcacggtct tgccgaggcc catgtcgtcg
     6061 gccaaacatc cgccgaggcc gaggcgcgtc agcagccaca gccaggcgac gccgtcgcgc
     6121 tggtacggcc gcagcacgcc gtgcagcgcg ggcccagggt cggcctcggc gttgccgctc
     6181 gggttctgca aggcggcgag caggctcgcc agccacgggc ccggacgcgc gacggtccag
     6241 cgcaccggcg tcgcctcggc gagcgagtcg cgcggcagcc cggcgagcag gcgcatgccc
     6301 tcggcgaagc cgagcgcgcc ctttgcagcg gccgcctcgg cgccctgcca gtggcgcagg
     6361 gcctcggcgt ggcgcgcagg ttcgagctcg acccagcggc cgcgcaggcg atgcaggccg
     6421 gcctcgccct gcatcagctc gcgccattcg tcggcggtca gctcgtcgtc g
//
LOCUS       DASWNJ010001907         3988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_598826, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001907 DASWNJ010000000
VERSION     DASWNJ010001907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598826"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2859
                     /locus_tag="VGB85_31815"
     CDS             <1..2859
                     /locus_tag="VGB85_31815"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0138730.1"
                     /translation="TGGGQSSAAADKVVAEIKAAGGEAVANYDSVEDGDKIVQNALDS
                     FGRIDIVVNNAGILRDSSFLKMTEADWDLIMRVHVKGAFKVTHAAWPRLRDQAYGRVI
                     FTSSAAGIYGNFGQANYSAAKLGLAAFGFTLAAEGRKYNIRVNTIAPIAGSRLTETIL
                     PKELIDALRPEYVSPLVAYLAHESCEETGGLFEVGGGFFGKLRWERTAGKLFRAGRDI
                     TPEMLAKSWASVVDFGKTTHPQDVTQAFQVVMDNVQSGPSKGGNDLIDVDQALGYEFP
                     PIKTKYDERDLSLYALGVGAAADPLDDNELRYVYEMHGKGFTPLPTYAVIPALTGVMN
                     AFREGQAAPGLNYGLDRLLHGEQYTELRRPLPPSAKLSHRTWVKDIFDKGKNAFVVMA
                     VETTDETGEVLAYNELGSVIRGAGGWGGDRGPTGDANTPPDRAPDFVRKEKIGDNQAL
                     LYRLSGDWNPLHVDPGFASAFGFAKPILHGLCTYGYAARHVLAALGADPKKFKSIKVR
                     FADSVFPGETLVTEIWKVSETRAVLRCKVAERDKLVISNAAIEFYTEVPNTAPKAQAA
                     KPSQAASTPAVPNSSDVFGAIGQFLQKNPDAAAKAGTVFQFKLSDPSSVWTVDCKANR
                     VAQGETAAPECTLELSDADFMAMCSGKADPQQLYMGGKLKIGGNLMASMKLNFLKKLE
                     PELVMSVMRGRIGTAAAAEVVPAAADQGPSSADVFVAIRDYLEKNPEVAAKVQTVFGF
                     KLKEPDSSWTIDLKSGKGSVQEGISGTPDCTLELKNSDWLAMTSGKADPQQLYMGGQL
                     KIAGNVMASTKLGFLKKIDMKAAQAAIAANRAKGSAAPATSAAPAAPKAAQAPAIADR
                     LGSKLAAAKSDTAVSLALRVKDPASEWSLTLGPQGGAIASGAADKPVATITIADDDLA
                     ALASGKTTTQRLFQQGKLRVDGDVLAAHHLSLVEGALS"
     gene            2880..>3988
                     /locus_tag="VGB85_31820"
     CDS             2880..>3988
                     /locus_tag="VGB85_31820"
                     /inference="COORDINATES: protein motif:HMM:NF006102.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HEY0138731.1"
                     /translation="MNDKVYVIGVGMVKFAKPGASDDYHVMAANASRAALKDAGVEYG
                     DIDQVVAGYVYGDSTCGQRAVYELGLTGVPVLNVNNNCSTGSTALFAGRQAVLSGEAQ
                     CVLVVGFEQMEKGALASKFNDRPTPLDPQANVMNEVQGITQAPPAAQMFGGAGREYRW
                     KHGTRRETFAKISEKARKHAANNPYALFKELLSVEEILASPEVFDPLTRYQCCPPTCG
                     AAAAVICSPEYAKKKGLQGGVYIAAQAMRTDYASSFEERSMIKMVGYDMAKHTASAVY
                     ERAGLGPKDVQVIELHDCFTANELLTYEALGLCPEGEAEKFIWDADNTYGGRWVTNPS
                     GGLLSKGHPLGATGLAQCTELVWQLRGTAEKRQVEG"
BASE COUNT          720 a         1340 c         1366 g          562 t
ORIGIN      
        1 accggcggcg gccagagttc ggccgcggcc gacaaggtgg tcgccgagat caaggccgcc
       61 ggcggcgagg cggtcgccaa ttacgactcg gtcgaggacg gcgacaagat cgttcagaac
      121 gcgctcgaca gctttggccg catcgacatc gtcgtcaaca acgccggcat cctgcgcgac
      181 agcagcttcc tgaagatgac ggaggccgac tgggacctga tcatgcgcgt gcacgtcaag
      241 ggcgcgttca aggtgacgca cgccgcgtgg ccgcgcctgc gcgaccaggc ctacgggcgc
      301 gtcatcttca cctcgtcggc ggccggcatc tacggcaact tcggccaggc caactacagc
      361 gcggccaagc tcgggctcgc cgcgttcggc ttcacgttgg cggccgaggg ccgcaagtac
      421 aacatccgcg tcaacaccat cgcgccgatc gccggctcgc ggctgactga gacgatcctg
      481 ccgaaagagc tgatcgacgc gctgcggccc gagtacgtca gcccgctcgt cgcctacctc
      541 gcgcacgaga gctgcgagga gaccgggggc ctgttcgagg tcggcggcgg gttcttcggc
      601 aagctgcgct gggagcggac cgcgggcaag ctgtttcgcg ccgggcgtga catcaccccc
      661 gagatgctcg ccaagagctg ggcgagcgtc gtcgacttcg gcaagaccac gcacccgcag
      721 gacgtcacgc aggcgttcca ggtcgtgatg gacaacgtgc agtccggccc gagcaagggc
      781 ggcaacgacc tgatcgacgt cgaccaggcg ctcggctacg agttcccgcc gatcaagacc
      841 aagtacgacg agcgcgacct ctcgctgtat gccctcggcg tcggcgcggc cgcggacccg
      901 ctcgacgaca acgagctgcg ctacgtctac gagatgcacg gcaagggctt cacccccctg
      961 ccgacctacg ccgtgatccc ggcgctgacc ggcgtgatga acgcctttcg cgagggtcag
     1021 gccgccccgg gcctcaacta tggcctcgac cgcctgctgc acggcgagca atacacggag
     1081 ctgcggcgcc cgctgccgcc gagcgcgaag ctgtcgcacc gcacgtgggt caaggacatc
     1141 tttgacaagg gcaagaacgc gttcgtcgtg atggccgtcg agacgaccga cgagaccggc
     1201 gaggtgctcg cgtacaacga gctcggctcg gtgatccgcg gcgccggcgg ctggggcggc
     1261 gatcgtgggc cgaccggcga cgccaacacc ccgccggatc gcgcgcccga cttcgtgcgc
     1321 aaggagaaga tcggcgacaa ccaggcgctg ctgtatcgcc tctcgggcga ctggaacccg
     1381 ctgcacgtcg acccgggctt cgccagcgcg ttcggcttcg ccaagccgat cctgcacggc
     1441 ctgtgcacct acggctacgc ggcgcgccac gtgctcgcgg ccctcggcgc cgacccgaag
     1501 aaatttaaga gcatcaaggt gcgcttcgcc gattcggtgt tccccggcga gacgctcgtc
     1561 accgagatct ggaaggtctc cgagacgcgc gcggtgctgc gctgcaaggt cgccgagcgc
     1621 gacaagctcg tgatctccaa cgccgcgatc gagttttata ccgaggtccc gaacaccgcg
     1681 ccgaaggccc aggctgcaaa accgtcgcag gccgcgagca cgccggcggt gcccaacagc
     1741 tcggacgtgt tcggcgcgat cggtcaattt ctgcaaaaga atcctgatgc cgccgccaag
     1801 gccggcaccg tgttccaatt taaactcagc gaccccagct ccgtgtggac ggtcgactgc
     1861 aaggccaacc gggtcgcgca gggtgagacg gccgcgccag agtgcacgct cgagctcagc
     1921 gacgcggact tcatggcgat gtgcagcggc aaggccgacc cgcagcagct gtacatgggc
     1981 ggcaagctga agatcggggg caatctcatg gcctcgatga agctcaactt cctcaaaaag
     2041 ctcgagcccg agctggtgat gagcgtcatg cgcgggcgga tcggcacggc cgcggccgcc
     2101 gaggtcgtgc ccgccgcggc cgatcagggc ccgagcagcg ccgacgtgtt cgtcgcgatc
     2161 cgcgattatc ttgagaagaa cccggaggtg gccgccaagg tccagaccgt gttcggcttt
     2221 aaattgaagg agccggacag cagctggacg atcgacctca agtccggcaa gggcagcgtg
     2281 caggagggga tcagtggtac gccggattgc acgctcgagc tcaagaacag cgactggctg
     2341 gcgatgactt ctggtaaggc cgacccgcag cagctctaca tgggcgggca gctcaagatc
     2401 gccgggaacg tcatggcctc gaccaagctc ggatttttga agaagatcga catgaaggcg
     2461 gcgcaggcgg ccatcgcggc caatcgcgcg aagggttcgg ctgccccggc gacctcggcg
     2521 gccccggccg cgccgaaggc tgcgcaggcg cccgcgatcg ccgatcgcct gggtagcaag
     2581 ctcgcggcgg cgaagtccga cacggccgtc agcctcgcgc tgcgggtcaa ggatcccgcg
     2641 agcgagtggt cgctgacgct cgggccgcaa ggtggtgcga tcgccagcgg cgcggccgac
     2701 aagccggttg cgacgatcac gatcgccgac gacgacctcg ccgcgctggc gagcggcaag
     2761 accaccacgc agagactgtt ccagcaaggc aagctgcgcg tcgacggtga cgtgctggcc
     2821 gcgcatcacc tctcgctcgt cgagggcgcg ctttcttgag tcacaggggg gatccgcaga
     2881 tgaacgacaa ggtctatgta atcggtgtcg gcatggtgaa gttcgccaag cccggggcga
     2941 gcgacgacta tcacgtcatg gccgccaacg catctcgcgc ggcgctcaag gacgccggcg
     3001 tcgagtacgg ggatatcgac caggtggtcg cgggctacgt gtacggcgac agcacctgcg
     3061 ggcagcgtgc ggtctacgag ctcgggctga ccggcgtgcc ggttctcaac gtcaacaaca
     3121 actgctcgac cggctcgacg gcgctgttcg ccggtcgcca ggcggtcctc agcggcgagg
     3181 cgcagtgcgt gctcgtcgtc ggcttcgagc agatggagaa gggcgcgctc gcctcgaagt
     3241 tcaacgaccg cccgaccccg ctcgacccgc aggcgaacgt gatgaacgag gtgcagggca
     3301 tcacgcaggc gcccccggcc gcgcagatgt tcggcggcgc cggtcgcgag tatcgctgga
     3361 agcacggcac gcgccgcgag acctttgcca agatctccga gaaggcccgc aagcacgccg
     3421 ccaacaaccc ctacgcgctc ttcaaagagc tgctgagcgt cgaggagatc ctcgcctcgc
     3481 cggaggtgtt cgacccgctg acgcgctatc aatgctgccc gccgacctgc ggcgccgcgg
     3541 cggcggtgat ctgctcgccc gagtacgcca agaagaaggg cctgcagggc ggcgtgtaca
     3601 tcgcggccca ggcgatgcgc accgactacg cgtccagctt cgaggaacgt agcatgatca
     3661 agatggtcgg ctacgacatg gcgaagcaca ccgcgagcgc ggtgtacgag cgcgccggcc
     3721 tcggcccgaa ggacgtgcag gtgatcgagc tgcacgactg cttcaccgcc aacgagctgc
     3781 tgacctacga ggcgcttggt ctgtgccccg agggtgaggc cgagaagttc atctgggacg
     3841 ccgacaacac ctacggcggc cggtgggtca ccaacccgtc cggcggcctg ctgtcgaagg
     3901 gccacccgct cggcgcgacc ggcctcgcgc agtgcacgga gctcgtgtgg cagctgcgcg
     3961 gcaccgcgga gaagcgccag gtcgaggg
//
LOCUS       DASWNJ010001908         3715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_629752, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001908 DASWNJ010000000
VERSION     DASWNJ010001908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629752"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="VGB85_31825"
     CDS             <1..859
                     /locus_tag="VGB85_31825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138732.1"
                     /translation="GGQPIGCQGRIDFLFVISSWYNMKGHQLQLQEAFTAFTAMLEDE
                     FADFDYHIMVVDAGTSALLEPICDDCYMCIGCKEEGCADFGGPPDYPCDEVKLECDVA
                     NGAGVTITGNFGATNKRCDLFGGHRYIVKGEPNPTDMFKCIATVGEGPKSPVPMQVMQ
                     AALQPDMLNGGCNDGFVRNDALLAVVILNADQDNHTPGPPQKWYDVLVAAKGGNEEAI
                     VTLVLSNDKELPDSVCEYEPIGPNPLRLFAEAAAHGRFESICLESYIPALKKTAATII
                     EQCSLLIPG"
     gene            complement(864..2618)
                     /locus_tag="VGB85_31830"
     CDS             complement(864..2618)
                     /locus_tag="VGB85_31830"
                     /inference="COORDINATES: protein motif:HMM:NF028473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138733.1"
                     /translation="MIRVLGLPSAPLLAAACLALPVPLACFGNVELEACTFTDPTGCD
                     EAASAAVSSTGAAPGDQSVTTSGTTGGNVDTTAADGSTEVTTTTTTTTDDAGTDTIGQ
                     PVDPPPTIADLVCDPEVADEVGPTVCTYTASADAVAADLLDDGQVIASGPASGSVIFP
                     VTSGPNNNPGSVITVTVRDEAGQTASESVYQASTVKEPGTEKWTTIEPNDGVFSSAGA
                     VALQGDFVIAAGVHYKNPQVIGTLRRYDQGGVWTPSDVGFSKSHGDWTKLPWLKSGNF
                     GPTGLAVDAEQNIILVGIGFENGDPRRYVARFHPDGALDWEVPGPVGTEARGVGVQPD
                     GTIYVAGAKRTSVNPERWDMEVSVYGADKTAYGPISYSDPGDTFNQRSERGRAVVVLK
                     SGRVVILGTREIVDLNDDQQFITRGVALLFEGKGKRVGAEWTSSGDKLMFDAILAGTA
                     TDEGFATCGYAHADPDEPGSKNQILIRWHGEDLQEVKAPRLETTQGTAVCNALGYNME
                     GATIVGAHVNVLGQNDNQWIFAVRDAADLRVDYLKHNGASNGPDRVQAIDCEYLCAWA
                     GIESVDGAVQWIAGLRRG"
     gene            2729..3607
                     /locus_tag="VGB85_31835"
     CDS             2729..3607
                     /locus_tag="VGB85_31835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138734.1"
                     /translation="MTLVAVYFALALAPPEPAGPAEPWREHEAACQTLAAAAASAKQG
                     VALAYAAAADQCRKAFETAASLASRSVFAFDAHRLYRRAHEAGHTDAICTDARMLKTF
                     AAQLAAPGAENRPHDRKDVDTLLSDLAPELVACSEPAESARPEPTTAVVPPASMGRTG
                     RADEMPRRSGPVSRPDRRPLRIAGGVALGLGLGLGGAMIGALVQGASLRTQADAVNGE
                     PGQTIGDGNDFASIRARGERADHMAIGFGVAAGALTVAGAALLVIDVRRKRAGGRLSL
                     QPSILPTAGIRLRLEF"
BASE COUNT          559 a         1302 c         1257 g          597 t
ORIGIN      
        1 cggcgggcag ccgatcggct gccaggggag gatcgacttc ctgttcgtga tctcgtcctg
       61 gtacaacatg aaggggcacc agttgcagct ccaggaggcg tttacggcct tcaccgcgat
      121 gctcgaggat gagttcgccg acttcgacta ccacatcatg gtcgtggacg cggggaccag
      181 cgcccttctc gaacccatat gcgacgactg ctacatgtgc atcggctgca aggaggaagg
      241 ctgcgcggac ttcggcgggc cgcccgacta cccctgcgat gaagtgaagc tggagtgcga
      301 cgtcgctaac ggcgcgggcg tgacgatcac cggcaacttc ggcgcgacga acaagcgctg
      361 cgacctgttc ggcggccacc gctatatcgt caaaggcgag cccaatccca cggacatgtt
      421 caagtgcatc gccaccgtgg gcgagggccc caagtcgccg gtgccgatgc aggtgatgca
      481 ggcggcgctt cagccggaca tgttgaacgg cggctgcaac gacggctttg tgcgcaacga
      541 cgccctcctc gccgtggtga tcctcaacgc agaccaggac aaccacaccc cgggcccgcc
      601 gcagaagtgg tacgacgtgc tcgtcgccgc caaggggggt aacgaggagg cgatcgtcac
      661 gctcgtgctc tcgaacgaca aggaattgcc agactccgtc tgcgagtacg agccgatcgg
      721 cccgaacccg ctgcgcctgt tcgccgaggc cgcagcgcac gggcgcttcg agagcatctg
      781 cctcgagagc tacatccctg cgctgaagaa gactgccgcg acaatcatcg agcagtgctc
      841 gctcctgatc ccggggtgac ccgctacccg cggcgaaggc cggcgatcca ctgcacggcg
      901 ccgtcgaccg actcgatgcc cgcccacgcg caaaggtatt cgcagtcgat cgcctgcacg
      961 cgatcggggc cgttgctcgc gccgttgtgc ttcaggtagt cgacccgcag atcggccgcg
     1021 tcgcgcacgg cgaagatcca ctggttgtcg ttctggccga gcacgttgac gtgcgcgccg
     1081 acgatcgtcg cgccctccat gttgtagccg agcgcgttgc acaccgcggt accctgcgtg
     1141 gtctccaggc gcggggcctt gacctcctgg agatcctcgc cgtgccagcg gatcaggatc
     1201 tggttcttgc tccccggctc atcggggtcc gcatgcgcgt agccgcaggt ggcaaagccc
     1261 tcgtcggtcg ccgtccccgc gaggatcgcg tcaaacatca gcttgtcgcc ggacgacgtc
     1321 cactcagcgc cgacgcgctt acccttaccc tcgaacagca gcgccacgcc gcgcgttatg
     1381 aactgttggt cgtcgttcag gtcgacgatc tcgcgggtgc cgaggatgac gacgcggccg
     1441 ctcttgagca cgacgacggc gcgcccgcgt tcgctgcgct ggttgaacgt gtcgcccggg
     1501 tcgctgtaag agatcggccc gtaggcagtc ttatcggcgc cgtagaccga gacctccatg
     1561 tcccaccgct cggggttgac gctcgtacgc ttcgcgcccg cgacgtagat cgtgccgtcc
     1621 ggctggacgc cgacgccacg ggcctcggtg ccgaccgggc cgggcacctc ccagtccagc
     1681 gcgccgtccg ggtggaagcg ggcgacgtac cggcgcgggt cgccgttctc gaagccaatg
     1741 ccgacgagga tgatgttctg ctcggcgtcc accgcgaggc cggtcggccc gaagttgcca
     1801 gacttgagcc acgggagctt ggtccagtcg ccgtgcgact tgctaaaccc gacatcactc
     1861 ggcgtccaca cgcccccctg gtcgtagcgg cgcagggtgc cgatcacctg ggggttcttg
     1921 tagtggacgc ccgcagcgat gacgaagtcc ccttgcagcg cgacggcacc cgccgagcta
     1981 aacaccccgt cgttcggctc gatcgtggtc cacttctcgg tgcccggctc cttgaccgtg
     2041 ctcgcctggt acaccgactc ggacgcggtc tgccccgcct cgtcgcgcac cgtcaccgtg
     2101 atcacgctgc ccgggttatt gttcggcccg ctggtgacgg ggaagatcac cgagccgctc
     2161 gccgggccac tggcaatcac ctggccatcg tcgaggagat cggccgccac cgcgtccgcg
     2221 ctggccgtgt aggtgcagac cgtggggccg acctcgtcgg cgacctcggg gtcgcagacc
     2281 agatcggcga tcgtcggcgg cggatcgacc ggctggccga tcgtgtcggt gccggcgtcg
     2341 tccgtcgtcg tcgtcgtcgt cgtcgtcact tcggtgctcc cgtccgcggc ggtcgtgtcg
     2401 acgttgccgc cggtcgtgcc cgacgtggtc acgctctggt cgcccggggc ggcccctgtg
     2461 gaggaaacgg cggcgctggc cgcttcgtcg cacccggtcg gatccgtgaa ggtgcatgcc
     2521 tccagctcga cattgccgaa gcacgcgagc gggaccggca gggccaggca agcggcggcc
     2581 aggagcgggg cggagggtag accgaggacc cggatcatgc ccgccagcat agcgcaagtc
     2641 cgcgcgtcct gtcgccccga gtgcccgagg gtggccagtg agtgtttact gaacgcgggc
     2701 ccatgcagat ccggtaggat gtgcggctat gaccctcgtc gccgtgtatt ttgccctcgc
     2761 cctggcccca ccggagcccg ccggcccggc cgaaccgtgg cgcgagcatg aggccgcctg
     2821 ccagacgctc gccgctgctg ccgcgagtgc gaagcaaggg gtggccctgg cctacgcggc
     2881 ggcggccgat cagtgccgca aggcattcga gaccgccgcg agcctggcga gtcgctcggt
     2941 gttcgcgttc gacgcccacc gcctctaccg ccgcgcgcac gaggccggcc acacggacgc
     3001 catctgcacc gatgcgcgca tgctcaagac gttcgcggcg caactcgccg cgcctggagc
     3061 cgaaaaccgg ccgcatgacc gcaaggacgt cgataccctg ctctcggatc tggcgccgga
     3121 gctcgtcgca tgctccgagc ccgccgagtc cgcgcgaccc gagccaacca ctgcagtggt
     3181 tccccctgcg agcatgggcc gcacgggccg agctgacgaa atgccccggc gatccggccc
     3241 ggtgagtcga ccggatcggc gtccccttcg gatcgcggga ggcgtggcgc tcgggctcgg
     3301 gctcgggctc ggcggcgcca tgatcggcgc gctcgttcag ggcgcctcac tccgcacgca
     3361 ggccgacgcc gtgaacggcg agccgggcca gacgatcggc gacggcaacg acttcgcatc
     3421 gatccgcgcg cggggcgagc gggccgatca catggcgatc gggttcggcg tcgccgcggg
     3481 ggcgctgacg gtcgctggcg cagccttgct cgtcatcgac gtccggcgca agcgtgcggg
     3541 cggccgactc tccctgcaac cgtccatcct gcccacggcg gggatccgtc tcagactgga
     3601 gttttagcga tgagcgaaga cgtcgacgtc ggcctacgtt tcccgcctaa ccatgacttc
     3661 tcgggcctgc tcctcggcca gcgctaccag ttggagcgac ggatcggctg tgggg
//
LOCUS       DASWNJ010001909         3776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_344845, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001909 DASWNJ010000000
VERSION     DASWNJ010001909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344845"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VGB85_31840"
     CDS             <1..199
                     /locus_tag="VGB85_31840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138735.1"
                     /translation="ATHRDELGRGQESNAHTWGKGTTHSESSIDPFNGFENQTTTRGP
                     DGTTTVTRSDGPTTTTLPNQL"
     gene            222..1319
                     /locus_tag="VGB85_31845"
     CDS             222..1319
                     /locus_tag="VGB85_31845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138736.1"
                     /translation="MSKVTIQRDICLPLIIPLTATHNFIPLPPFPPIPPPLPAMPLSL
                     AACAIEAPVNAWWPPGGAFGSHKFTSTVFHNGMQICLDGHDCGKFIPHLQVMPAPNNM
                     LTFIHIPFSSRKANFSSASVLMNGSPTACMTMIAWPPTPMTYCAEPLSMPLADAPTSH
                     FNTVEVRMSLADWLAGALNIAVGMMLEWVTRTSSKDFGKAAGKDIARSRAGQSTASLI
                     RERFINQIKGQFDMQWLAKQTIGALTGAAQIALTGQGSISVNAPGGGGFFSLGGSVSR
                     SGGEWGGGVSGSAATVGGSVDNKGASATSRDPLGRGQDNTAHEWGKGTTTTESRTDPF
                     NGFQNTTTTRGPDGTTVTRSDGPTTSLPNQL"
     gene            1323..2540
                     /locus_tag="VGB85_31850"
     CDS             1323..2540
                     /locus_tag="VGB85_31850"
                     /inference="COORDINATES: protein motif:HMM:NF021456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HEY0138737.1"
                     /translation="MTDPKPLREPTHDQYDVVIDFFPPEAMPEFVYALVKLTYDIKDG
                     RLERSAVQPLYHDIRDPNLKPRWARGSDFWPIKLWTDVVVRGSAYGPEGRAVRSQRVV
                     VTVGDGGKAIQVYGDRVVEWPAHGQLRFGAPEPFTKMPLDWDRAYGGWDRRVDPGFGP
                     NPTVAELSRLEFDHPGMYPRNPFGCGYIVVDEPVDGVPLPNLEDPAQLLTPETFVTGD
                     PRRWYRQPLPACFEFTTAMFFHRLCWLGSEAWFHPPEGAPIAEVELGVLPPDYHLLRG
                     GLNQAPQAMQEAPIGLVFDPLDAGTPIAVEGMHPELRRIPFELPAPPRLEFHIDGKTY
                     PVEPQLTNVLIEPDSPRVSLTYVARQFDLPRVFVPGIHAKIPIELRIEGIGTIAYVTP
                     PTLRELRRGTAPT"
     gene            complement(3169..>3776)
                     /locus_tag="VGB85_31855"
     CDS             complement(3169..>3776)
                     /locus_tag="VGB85_31855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138738.1"
                     /translation="GKPRRSVDPTVTIEEPATRTALEDLLDAPVARRVATPTGGCLIA
                     SCVEERHPTLQGRVQVQLVDGEGHPHTLWVPTLQGLPVRKGDRLLLVRPGNGDEWIAT
                     GVVDGFARRPEVSKVPAAQLELKSDECVQVVSSAGQPLVEVSQGSAGPVVRLLEPDVR
                     VEFAGKLVLRAEGIELEATGGQVEIKASEDVVIKGELVRLN"
BASE COUNT          573 a         1533 c         1145 g          525 t
ORIGIN      
        1 cgccacccac cgcgacgagc tcggccgcgg ccaggagagc aacgcgcaca cgtggggcaa
       61 gggcaccacg cacagcgaga gcagcatcga cccgttcaac ggcttcgaga accagacgac
      121 cacccgcggc cccgacggca ccaccaccgt cacgcgcagc gacggcccga cgacgaccac
      181 gctgcccaac cagctgtgaa tcggcgagga gagcaaccgc gatgagcaag gtgacgatcc
      241 agcgcgacat ctgcctgccc ctcatcatcc cgctgacggc gacgcacaac ttcatcccgc
      301 tgccgccgtt tcccccgatc ccgccgccgc tgcccgccat gccgctgtcg ctggccgcgt
      361 gcgcgatcga ggccccggtc aacgcctggt ggccgcccgg cggcgcgttc gggtcgcaca
      421 agttcaccag caccgtgttt cacaacggca tgcagatctg cctcgacggc cacgactgcg
      481 gcaagttcat cccgcacctg caggtcatgc cggcgccgaa caacatgctg acgtttatcc
      541 acatcccgtt ctcctcgcgc aaggccaact tcagcagcgc cagcgtgctg atgaacggct
      601 cgccgaccgc gtgcatgacg atgatcgcct ggccgccgac cccgatgacc tactgcgccg
      661 agccgctcag catgccgctg gccgacgcgc cgacgagcca cttcaacacc gtcgaggtcc
      721 gcatgtcgct cgccgactgg ctcgccggcg ccctcaacat cgccgtcggc atgatgctcg
      781 agtgggtgac gcgcacctcc tccaaggact tcggcaaggc cgccggcaag gacatcgccc
      841 gcagccgcgc cggccagagc accgcctccc tgatccgcga gcgcttcatc aatcagatca
      901 agggccagtt cgacatgcag tggttggcga agcagaccat cggcgccctc accggcgcgg
      961 cgcagatcgc cctgacgggt caggggtcga tcagcgtcaa cgcgcccggc ggcggcggct
     1021 tcttctcgct cggcggctcc gtctcgaggt ccggcggcga gtggggcggc ggcgtgtccg
     1081 gcagcgccgc gaccgtcggc ggcagcgtcg acaacaaggg cgccagcgcc accagccgcg
     1141 acccgctcgg ccgcggtcag gacaacacgg cgcacgagtg gggcaagggt accaccacca
     1201 ccgagagccg caccgacccc ttcaacggct tccagaacac gaccaccacc cgcggccccg
     1261 acggcacgac cgtcacgcgc agcgacggcc cgaccacctc gctgcccaac cagctgtgac
     1321 tcatgacgga ccccaagccc ctgcgcgagc cgacccatga ccagtacgac gtcgtcatcg
     1381 acttcttccc gccggaggcg atgcccgagt tcgtctacgc gctcgtcaag ctgacctacg
     1441 acatcaagga cggccgcctc gagcgctccg cggtccagcc gctgtaccac gacatccgcg
     1501 atccgaacct caagccgcgc tgggcccggg gcagcgactt ctggccgatc aagctctgga
     1561 ccgacgtcgt cgtccgcggc agcgcctacg gccccgaggg tcgcgccgtc cgctcgcagc
     1621 gggtcgtcgt caccgtcggc gacggcggca aggcgatcca ggtctacggc gaccgcgtcg
     1681 tcgagtggcc cgcgcacggc cagctccgct tcggcgcgcc cgagcctttt actaaaatgc
     1741 cgctcgactg ggaccgcgcc tacggcggct gggaccgccg cgtcgacccc ggcttcggcc
     1801 ccaacccgac cgtcgccgag ctctcgcgcc tcgagttcga ccaccccggc atgtacccgc
     1861 gcaacccctt cggctgcggc tacatcgtcg tcgacgagcc cgtcgacggc gtcccgctcc
     1921 ccaacctcga ggaccccgcg cagctgctga ccccggagac ctttgtcacc ggcgacccgc
     1981 gccgctggta tcgccagccg ctgccggcct gcttcgagtt cacgaccgcg atgttctttc
     2041 atcgcctgtg ctggctcggc tccgaggcct ggtttcaccc gcccgagggc gccccgatcg
     2101 ccgaggtcga gctcggcgta ttgccccccg actaccacct gctgcgcggc ggcctcaacc
     2161 aggccccgca ggcgatgcag gaggccccga tcggcctcgt cttcgatccc ctggacgccg
     2221 gcaccccgat cgccgtcgag ggcatgcacc ccgagctgcg ccggatcccc ttcgagctgc
     2281 ctgccccgcc gcggctcgag ttccacatcg acggcaagac ctaccccgtc gaaccccagc
     2341 tgaccaacgt cctcatcgaa cccgactccc cgcgcgtctc gctgacctac gtcgcccgcc
     2401 agttcgacct cccccgcgtc ttcgtgcccg gcatccacgc caagatcccc atcgagctgc
     2461 gcatcgaagg catcggcacg atcgcctacg tcaccccccc gaccctccgc gagctccgcc
     2521 gcggcaccgc cccgacctga ccccgccctc gtcgctgcgc gcctcgccct cgcagacccg
     2581 gcgcgagcat cgtgtggagc tggcgcgtcc gtgctccgcg gggcgggcgg catcgtgtac
     2641 ggcggaaggg gggccgacgc gaccgggtcc ctcgcgacgg ccggtgcctc cccacgcaca
     2701 tgctcggcgc ctgtccgcga tgcagcgtca ttcatcccga acctgtcctc gcggacatac
     2761 gactgtgcgc ctgcgcgtga gcgcggggcc cctccggcct gcgtcccggg ctgtcggccc
     2821 ctcgtccgcc gagcggcacc gtcggcgccc cgctcatctc caggccacta tcagctcaag
     2881 gcgaacgcct catactcgcg catcgcagca tcctcgagtc gacgtgcaga ctgcgcgccc
     2941 ggcgtggaag cattcgcgta ccagcactcc ccgacccacg ccaagctgtc cgtagggaca
     3001 tgcccgcgcc gccataccgc gccccgaggc ccggtggggt ccggctctcg gcagggccga
     3061 ccgcaccgtc ctttagcgca ggcgtcggtg gcggaggacc gaaggaggtt tcgaccgagt
     3121 ccgacgggcc ccgggtcttc ggacgcacac gccaacgccg agcgaggcct agttgagccg
     3181 cacgagctcc cctttgatga cgacatcctc gctggccttg atctccacct gccccccggt
     3241 cgcctcgagc tcgatcccct ccgcccgcag caccagcttc ccggcgaact ccacacgaac
     3301 gtccggctcg agcaaccgca ccaccggccc cgcgctcccc tgcgagactt ctacgagcgg
     3361 ctgccccgcg ctcgacacca cctgcacgca ctcgtcgctc ttgagctcga gctgcgccgc
     3421 cggcaccttg ctcacctcgg gccggcgagc gaagccatcg accacgcccg tcgcgatcca
     3481 ctcgtcgccg ttgccggggc ggaccagcaa caggcggtcg cccttgcgga ccggcagccc
     3541 ttgcagcgtc ggcacccaca gcgtgtgcgg gtgtccctcg ccgtcgacga gttgcacctg
     3601 cacacgccct tgcagggtcg ggtggcgctc ctccacgcag ctggcgatca ggcagccgcc
     3661 ggtgggcgtc gccacgcgtc gtgcgacggg ggcgtcgagc agatcctcga gggccgtgcg
     3721 cgtcgcgggc tcctcgatcg tcaccgtcgg atcgactgac ctgcgtggtt tgccct
//
LOCUS       DASWNJ010001910         2764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_173614, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001910 DASWNJ010000000
VERSION     DASWNJ010001910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173614"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VGB85_31860"
     CDS             <1..346
                     /locus_tag="VGB85_31860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138739.1"
                     /translation="DPGKEDPGTDTGEPGKEVKKTGGTPRARPDPKSDACKNTRDKVN
                     AARTSGNFHDMLRFLNERDCWAAKSEHGKLKTLAYKELGKFKECEKAGKGLTDPDVVK
                     WVNLCAKRAQSG"
     gene            666..1610
                     /locus_tag="VGB85_31865"
     CDS             666..1610
                     /locus_tag="VGB85_31865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138740.1"
                     /translation="MAWAAWMAPAATPAAAAGADACAKLEFRAILQVDTQALSDDAQG
                     VGSRLRSKAEQEIQRFDVMLGEDARLPKVVIKIMPMTGEDEGFSYTIDINHLELTPIK
                     DGSSVGECKLCTESELLEKVAGSTRALMPKLRAHITDFNNRPCETAAPRCDSNAQCKD
                     PARPVCHVREKRCVADLPPGCNTDTDCAKSPVGPFCSEQSHRCAATRDSAPPDTGMNG
                     KQKAGIGLMVGGALGAGIGIGLVVAKPRVPEPTMAWETRETQKPGFAVLAIGGAALIT
                     GVVLFVLGRRQQKAQHASHQFAPVAGAGVFGLSWSGRF"
     gene            1743..>2764
                     /locus_tag="VGB85_31870"
     CDS             1743..>2764
                     /locus_tag="VGB85_31870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138741.1"
                     /translation="MLKLRNGLALGAFGLSLGTLAGCAVTNLEHCALNQGACGEGLAC
                     SMCAVDNNGCVPLGALLEDACVFDSGGSDPSTVTSMQTTTSDPTTVTPTESTDTSAET
                     IDLTTSTSDTSTTGLTITTSDTTDEETATTEPVACEGQVADNPKCGGEAPYCVKNTCV
                     GCAKLGDLSCAEVEEDKPTCAADIGLCVECLTHADCTTAEAPACDAATANCVPCDSHD
                     QCPATACNLETGQCFPNNNVLYVENSPADPVECSDVKPDYGTTPETPLCTLQNAISRV
                     TQGVEWTVKIKTGQKQQNGITEIPVGNYTVAIMHYGNTVPSLIISFADPALTVNEGNT
                     VFMNRVG"
BASE COUNT          499 a          981 c          919 g          365 t
ORIGIN      
        1 cgatccgggc aaggaagatc cgggcaccga caccggcgag cccggcaagg aggtcaagaa
       61 gaccggcggc acgccgcgcg cgcgccccga ccccaagagc gacgcgtgca agaacacgcg
      121 cgacaaggtc aacgcggcac gtacttcggg caacttccac gacatgctgc gcttcctcaa
      181 cgagcgcgac tgctgggccg cgaagagcga gcacggcaag ctcaagacgc tcgcatacaa
      241 ggagctcggc aagttcaagg agtgcgagaa ggcgggcaag ggcctcacgg accccgacgt
      301 cgtcaagtgg gtcaacttgt gcgcgaagcg ggcgcagtcc ggctgacgcg ctgtgccgcg
      361 cgagacggcc gtcgcgcgca tgcacctgcg ccaaactcga gggtctagcc gggcatacga
      421 acgcggacgc tcgctcgcgc gcgaacccgc tcgaccaaac tcgcgccgct gatccaggcg
      481 ggatcaggcg ggctcgtggc cgggcgcggg ggtggacgcg gccctggcga gcgggcacaa
      541 tgcggcccgg ctcgcccgct tttacggcca tgttcgtcgc cactacgctc gcaatttcgc
      601 tcgccacctc gctcgcactg tcgcctgtca ccacgctggg cgctgcgccg cggagccacg
      661 caaccatggc ctgggcggcc tggatggcgc cggcggcgac gccagctgcg gctgcagggg
      721 ctgacgcctg cgccaagctc gagttccggg cgatccttca ggtcgacacc caggcgctca
      781 gcgacgacgc gcagggcgtc ggtagccgcc tgcgcagcaa ggccgagcag gagatccagc
      841 gcttcgacgt catgctcggc gaggacgccc ggctgcccaa ggtcgtcatc aagatcatgc
      901 cgatgaccgg cgaggacgag ggcttcagct acacgatcga catcaaccac ctcgagctga
      961 cgccgatcaa ggacggcagc tcggtcggcg agtgcaagct gtgcaccgag agcgagctgc
     1021 tcgagaaggt cgccggctcg acgcgcgcgc tgatgccgaa gctgcgcgcc cacatcaccg
     1081 acttcaacaa tcgcccgtgc gagacggccg cgccgcgctg cgacagcaac gcgcagtgca
     1141 aggaccccgc ccggccggtc tgccacgtcc gtgagaagcg ctgcgtcgcc gacctccccc
     1201 ccggctgcaa caccgacacc gactgcgcca agagcccggt cggcccgttc tgcagcgagc
     1261 agagccaccg ctgcgccgcc acgcgggaca gcgccccgcc cgacaccggc atgaacggca
     1321 agcagaaggc aggcatcggc ctgatggtcg gcggtgcgct cggcgctggt atcggcatcg
     1381 gcctcgtcgt cgccaagccg cgtgtccccg agccgacgat ggcgtgggag acccgcgaga
     1441 cgcaaaagcc cggcttcgcg gtgctcgcca tcggcggcgc cgcgctgatc accggcgtcg
     1501 tgctgttcgt gctcggccgc cggcagcaga aggcgcaaca cgccagtcac cagttcgcgc
     1561 cggtcgccgg cgcgggggtc ttcggcctca gctggtcggg tcgcttctag tggttttgcc
     1621 ggggcccttt ggctccgggt ggtttccctg cgtcttatcc ggccttcggg cctttaccag
     1681 tgagcataga cccggtcgct cgacgaccgc gtgaggcacg agccatgcga tctttctgga
     1741 ccatgttgaa gcttcggaat ggactggcgc tcggcgcgtt tgggctctcg ctcggcactc
     1801 tggccggctg cgccgtgacc aacctcgaac actgcgcgct caaccagggc gcgtgcggcg
     1861 agggcctcgc gtgcagcatg tgcgcggtcg acaacaacgg ctgcgtgcca ctgggcgcgt
     1921 tgctcgagga cgcgtgcgtg ttcgacagcg gcggctccga cccgagcacc gtcacgagca
     1981 tgcagacgac caccagcgat ccgacgaccg tcacgccgac cgagtcgacc gacacctccg
     2041 cggagacgat cgacctgacg acctcgacct cggacaccag caccaccggg ctcacgatca
     2101 ccaccagcga caccacggac gaggagaccg ccaccaccga gccggtcgcc tgcgaaggcc
     2161 aggtcgccga caacccgaag tgcggcggtg aggccccgta ctgcgtcaag aacacctgtg
     2221 tcggctgcgc caagctcggt gacctcagct gcgccgaggt cgaggaggac aagccgacct
     2281 gcgcggcgga catcggcctg tgcgtcgagt gcctgacgca cgccgactgc acgacggccg
     2341 aggcgccggc ctgcgacgcc gcgaccgcga actgcgtccc gtgcgacagc cacgaccagt
     2401 gccccgcgac cgcgtgcaac ctcgagacgg ggcagtgctt cccgaacaac aacgtcctct
     2461 acgtcgagaa ttcgccggcc gatccggtcg agtgcagcga cgtcaagccc gactacggta
     2521 cgacgccaga aacgccgctg tgcaccttgc aaaacgcgat atcgcgggtg acccagggcg
     2581 tcgagtggac cgtcaagatc aagaccggcc aaaagcagca aaacgggatc accgagatcc
     2641 cggtcggtaa ctacacggtg gcgatcatgc actacggcaa cacggtgccg tcgttgatca
     2701 tctcgttcgc cgatccggcg ctgacggtca acgagggcaa caccgtcttc atgaaccgcg
     2761 tcgg
//
LOCUS       DASWNJ010001911         1725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_527689, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001911 DASWNJ010000000
VERSION     DASWNJ010001911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527689"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1417)
                     /locus_tag="VGB85_31875"
     CDS             complement(<1..1417)
                     /locus_tag="VGB85_31875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEY0138742.1"
                     /translation="MLDLLRRLLAAPTFADEEDTRVASLLRTILLGSAGISAVAGLAQ
                     FAGLGHRQPILLIYAGVVVLSLGLFALVRRGHIQLAGTGFLLVLWVAITLGVLTYGGI
                     RSPSAGGYILVVIISTLLFSGRLVLAFLGLSLATLAAIVVVELAGALPPQLTPDTPQV
                     AFIAHFIHLISAGFFLYLAVKNLQEARQRARRGAAKAAELLREATSAKNYADNILASM
                     AESLVVLDPRGTIVTVNKATLDLLGYDAGQLLGQPFKRVLPDFGAFASDSTPTSGTHE
                     PVERSYRNAAGSPVPVLFSQARLPGEHSGRAGSVCVASDITQLKHAENSLREAKQLAE
                     EASLAKSRFLANMSHELRTPLNAVIGYAEMLLEEADERGIVGSEEEIRKIQFAGKHLL
                     GLISDILDLSKIEAGRMDIHLETFDVADMIDTVLGTIGPMIARNGNQITVHCPPSIGF
                     IHADLTKIRQILLNLLSHAAKF"
BASE COUNT          284 a          515 c          664 g          262 t
ORIGIN      
        1 tgaacttggc ggcgtgagag agcaggttga gcaagatctg gcggatcttg gtcaggtcgg
       61 cgtggataaa gccgatcgag ggcggacagt gcacggtgat ctggttgccg ttgcgggcga
      121 tcatcggacc gatggtcccg agcacggtgt cgatcatgtc ggcgacatca aaggtctcga
      181 ggtggatgtc catgcggccg gcctcgatct tcgagaggtc gaggatgtcc gagatcaggc
      241 cgagcaggtg cttgccggcg aattggatct tgcggatctc ctcctccgag ccgacgatgc
      301 cgcgctcgtc ggcctcctcg agcaacatct cggcgtaacc gatgacggcg ttgagcgggg
      361 tgcgcagctc gtggctcatg ttggcgagga agcggctctt ggcgagcgag gcctcctcgg
      421 cgagctgctt ggcctcgcgc aggctgttct cggcgtgctt gagctgggtg atgtccgagg
      481 cgacgcacac cgagccggcg cggccgctgt gctcgcccgg cagccgcgcc tgcgagaaca
      541 gcaccggcac cggcgagccc gcggcgttgc gatagctgcg ctcgacgggc tcgtgcgtgc
      601 cggaggtcgg ggtgctgtcg ctggcgaagg ccccgaagtc gggcagcacg cgcttgaacg
      661 gctggccgag cagctgcccg gcgtcgtagc cgagcaggtc gagggtcgcc ttgttgacgg
      721 tgacgatcgt gccacgcggg tcgagcacga ccagcgactc ggccatcgag gcgaggatgt
      781 tgtcggcgta gttcttggcg gaggtcgcct cgcgcagcag ctcggccgcc ttggcggcgc
      841 cgcgacgggc ccgctggcgc gcctcctgca ggttcttgac ggcgaggtag aggaagaagc
      901 cggcgctgat caggtggatg aagtgggcga tgaaggcgac ctgcggggtg tcgggggtca
      961 gctgcggcgg caaggcgccg gcgagctcga ccacgacgat cgccgcgagc gtggcgaggc
     1021 tgaggccgag gaacgcgagc acgaggcgcc cgctgaacag cagcgtcgag atgatgacga
     1081 cgaggatgta accgccggcg ctcgggctgc ggatcccgcc gtaggtgagc acgccgaggg
     1141 tgatcgcgac ccacagcacg agcaagaagc cggtgcccgc gagctggata tggccgcgac
     1201 ggaccagcgc gaacaggccg aggctgagca cgacgacgcc ggcgtagatc agcaggatcg
     1261 gctgccggtg gcccaggccg gcgaactggg cgagccctgc gaccgcgctg ataccggcgc
     1321 tgccgagcag gatcgtgcgc agcagcgacg cgacccgggt gtcctcttca tctgcaaacg
     1381 tgggcgcagc cagaagtctg cggagaaggt ccaacatccg ggcccactcg acgctacggg
     1441 ccgcaccgtc cggtgtcaag gatcgcgagg gtgcacacac tgcgtgcgcg cttgtacgcg
     1501 cacgtgatgt cgtcgcgaga tgtggtccgt ggcgagaggc cgtcgctacg acctagaagc
     1561 ggaccttgag gtgacccgcg atcgtcgcaa ggaacagctt gtaggtgccg ttgccgaggt
     1621 cgaagtcgga ggtcgtcacg tggcgcggga cgatgccctg gaagcgggcg tctgcgagga
     1681 ggctgacgcg ttcggtgatg tcgaggccgc caccgacgcc gccga
//
LOCUS       DASWNJ010001912         7670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_173671, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001912 DASWNJ010000000
VERSION     DASWNJ010001912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 7670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7670
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173671"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5931)
                     /locus_tag="VGB85_31880"
     CDS             complement(<1..5931)
                     /locus_tag="VGB85_31880"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0138743.1"
                     /translation="MEVTTRIKSRLTEVTEWNGVIDEFESQASSAGSAQAQSAAFFQL
                     AQACEGIFLDKARAMTFYQTAVKRDRENLLALQQARSIYQQMANLEMVIKLMALELRA
                     NRDPARAPILNYAYGRALLNLRQVDNARPYLEAAASAESRNTEYQDRFQETLYDRGNW
                     QFALQNIYNQLAALTAQSDPLAANVENAGHTLSTLFMRAARILQQEAPDDQRLLPLLF
                     KALDANPLNHEAAYVAETMLAAGGHLQHIQKLQDRRVALIQEPADKVAILRDFALIWQ
                     VRLNNADMASYFYRQALEYAYSIGGETLRDPQGGDWHLGAFYLLKKTAERTGSAEGLV
                     ELGARGLSVISDPIDAALVAITSGEIAWKLRNDVETARSLLGWAASVAANHPILREFT
                     ASHGPIAIADTSEQQAAAQRADEERAAASRAAEERAASERAATERAEREASERAAREA
                     SERGAREESERAAREASERSAREASERAAREAAQADDGVAERAAAERAAAERAAAEQA
                     ERAASERAASERAAADKAAADKAASERAAADKAAADKAAADKAATDKKAAADKAAADK
                     AAAERAAAERAAAEAAAIPGEAIEIGGEVFSPEEQRFLNAARTAEAAGGTKAIDGWRD
                     AVQKLPGKRYPRTRLRELYAESGKWSNVADLLKEELKQDPTRDPEYTKAAYWQLIEIY
                     RDHLRQPGLVVTTLAALEKVLEDSGDTEALLKVVETQQAQFDTMKRWPDLIGRIRRRA
                     ELTQDPAGRTALHLEAGRLFLDKFNNQAEAIKSFESVLEADEYNAEAISKLKDLYAKR
                     RDWEKMVHVQQKELTLLADPAQRMEQLLDIARTAVAKIKKNPLSIELWNQVLATDPGN
                     HEALEQLEGLLEREKEWAQLSRVLNTLTEVEKDNAKRVQHLIKLGSLYSDKLDDQKSA
                     ITAWETLYRLDPENRRAQDALKKLYLVVGDMDSLQEFYAKQDKWGEFVRVLEKESETA
                     EGAQRTTLLLKIADLYHTRLEKADKATRALEKALSFDDNNLTVANALFALYEEAHDER
                     HISVPLKIQLNHSEDPEQRNLLLRRLADLAERVAHDQALAFGYYRQAFTENHTQSDIF
                     DHMVRLGEEIGAWGELVESLRAGIEKYGSDPESIPLRLKLAEIYEKRVSDLDAALAAN
                     QAILEINGEEPTALTSLERLYLALGREEDLLNILRTKLSLTHSDDERRGIQTHIGSIL
                     EQAGHIEQAVAAYEAVLATGVEDLGALGALDRMYLTQGKWNELADILRRELAATGTDN
                     TEARGTFLLRLGVLTQDKLNNAADAVELYRQVLEVDPTNEEARRRLEGWLDDDALKLT
                     VATILLPVYEHLQAWPQVVQTLEIQVAAAGSASERVDLLLRIGSILAQTIGNSAGAFD
                     AYSRAFRQDPHNETAQEELEKIAQIEDRFADFAALYEEAVAGDLPSDLQRSLLMRLAA
                     LYDQRLGNAPKAIENYRKALEVDADNVAALEALDKLYSRDQNWAELLGVYRSKVALTA
                     EPAARQDLRFRIAYLQDEMLGQKEEAIATYNEILADDFENLQAIVALDRLYLARGAWT
                     ELAENLERQLTLSHDPTAQVELNLRLGELRLTRLDQAAQAVETYNRALAIDPSNATAL
                     AALETLLTNESQQLTVARVLLPYYNAANNWPRLIQSYEIMVAHSQEPEEKIQLLHRIA
                     ELQETGLDDQSKAFEALGRAFKVDPANPLTQERLEGLARQMGAYQQLVALYDESIPDI
                     VDDQLIIRLLVKIAQIHETILADAAKAAASYDRILGIDPANFEAVDALIQVHINQNNF
                     EALVNAVVRKSEMVSSVDDRKSLLLYAAQIREVQMTDLPGAIGLYQQVLAIDDADAAA
                     LDALDKLYVQTEAWEPLRDIYRRKTELAAEPEYRAHWLHLLGQVYDQKLGDVEHAIET
                     YQAILDIQPADYHAIQALDRLFGQAERWSDQLSILERAVDVAPAREEKTELRHRIGAL
                     WENQLADMVRAVEAYRETL"
     gene            complement(6314..7222)
                     /locus_tag="VGB85_31885"
     CDS             complement(6314..7222)
                     /locus_tag="VGB85_31885"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0138744.1"
                     /translation="MRRLPLIAALLVSFVIVLPGCNLASRNRVQAINRMNEGIQLFDK
                     NNSPGAEKALQDAIQLDPTYAAAYHTLGQIYKKQNKLVDAEKAFQGAIDNMKSEPNAE
                     YYYDLGAIQSSQGEADGVASSERETKFRAAINSFQEALKLDPKVYKAHYRTGTLHEKL
                     DEPEKADAAYRKTIEIKPSYSPAFVSLGNMYIDYGFANVAMVVLQTGAQINDKDARMW
                     NGLGRAHFELGQFQEAIDAYSKAKAIDPDGVDALYGLGMAYAELQKRKEAKENLELFI
                     SKAGQETRPDLVKAARDTVARMADVI"
     gene            complement(7531..>7670)
                     /locus_tag="VGB85_31890"
     CDS             complement(7531..>7670)
                     /locus_tag="VGB85_31890"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEY0138745.1"
                     /translation="VVQETTMKDAGVGNCIAQAVKRWTFPKPEGGGSVIVTYPFVLEP
                     G"
BASE COUNT         1040 a         2380 c         2849 g         1401 t
ORIGIN      
        1 gagggtctcg cggtaagcct cgacggcgcg gaccatgtcc gcgagctggt tctcccacag
       61 agcaccaatg cggtggcgca gctcggtctt ctcctcgcgg gcgggcgcca cgtcgacggc
      121 gcgctcgagg atgctgagct ggtcgctcca gcgctcggcc tggccgaaca ggcgatcgag
      181 cgcctggatg gcatggtagt ccgcgggctg gatgtcgagg atcgcctggt aggtctcgat
      241 cgcgtgctcg acgtcgccga gcttctggtc gtagacctga ccgagcaggt gcagccagtg
      301 ggcgcggtac tccggctcgg cggcgagctc ggtcttgcgg cggtagatgt cccgcagcgg
      361 ctcccacgcc tcggtctgga cgtagagctt gtcgagggcg tcgagcgcag cggcgtcggc
      421 gtcgtcgatg gcgaggacct gctggtacag cccgatcgcg ccgggcaggt cggtcatctg
      481 gacctcacgg atctgggcag cgtagagcag caggctcttg cggtcgtcga ccgagctgac
      541 catctccgac ttgcggacca ccgcgttgac gagggcctcg aagttgttct ggttgatgtg
      601 gacctggatc agggcgtcga cggcctcgaa gttggcgggg tcgatgccga ggatgcgatc
      661 gtaggacgcc gcggccttgg ccgcgtcggc gaggatcgtc tcgtggatct gggcgatctt
      721 gacgagcagg cggatgatca gctggtcgtc gacgatgtcg gggatcgact cgtcatacag
      781 ggccacgagc tgctggtaag cgcccatctg ccgcgcgagg ccctcgaggc gctcctgcgt
      841 cagggggttg gccgggtcga ccttgaacgc gcgaccgagg gcctcgaagg ccttgctctg
      901 gtcatcgagc ccggtctctt gcagttcggc gatgcggtgc aggagctgga tcttctcctc
      961 gggctcctgc gagtgggcga ccatgatctc gtacgactgg atcaggcgcg gccagttgtt
     1021 ggcggcgttg tagtacggca gcaacacgcg agcgacggtc agctgctgcg actcgttggt
     1081 cagcagggtc tcgagagcag caagcgcggt cgcgttgctc gggtcgatcg ccagcgcgcg
     1141 gttgtaggtc tcgacggcct gcgcggcctg gtcgagccgc gtcaggcgca gctcgccgag
     1201 gcggaggttg agctccacct gggcggtcgg gtcgtgcgag agggtcagct ggcgctcgag
     1261 gttctcggcc agctcggtcc acgcgccgcg cgcgaggtac aggcggtcga gggcgacgat
     1321 cgcctgcaag ttctcgaagt cgtcggcgag gatctcgttg taggtcgcga tcgcctcttc
     1381 cttctggccg agcatctcgt cctgcaggta agcgatgcgg aagcgcaggt cctggcgcgc
     1441 ggccggctcg gccgtcagcg cgaccttgct gcggtagacg ccgagcagct cggcccagtt
     1501 ctggtcccgg ctgtacagct tgtcgagcgc ctcgagggcg gcgacgttgt cggcgtcgac
     1561 ctcgagcgcc ttgcggtagt tctcgatcgc cttgggggcg ttgccgaggc gctggtcgta
     1621 gagcgcggcg agccgcatca gcaggctgcg ctggaggtcg ctcggcaggt cgccggcgac
     1681 ggcctcctcg tagagggccg cgaagtcggc gaagcggtcc tcgatctggg cgatcttctc
     1741 gagctcctcc tgggcggtct cgttatgcgg atcctggcgg aacgcgcggg agtaggcgtc
     1801 gaaggcgccc gcggagttgc cgatcgtctg cgcgaggatc gaaccgatgc gcagcaggag
     1861 atcgacgcgc tcgctggccg agccggccgc ggcgacctgg atctcgaggg tctggacgac
     1921 ctgcggccag gcctggaggt gctcgtagac ggggagcagg atggtcgcga cggtgagctt
     1981 gagggcgtcg tcgtcgagcc agccctcgag ccggcggcgg gcctcctcgt tggtcgggtc
     2041 gacctcgagg acctggcggt acagctcgac cgcgtcggcg gcgttgttga gcttgtcctg
     2101 ggtcaggacg ccgaggcgca gcaggaaggt cccgcgggcc tcggtgttgt cggtgccggt
     2161 ggcggcgagc tcacgacgga ggatgtccgc cagctcgttc cacttgccct gcgtcaggta
     2221 catgcggtcg agggcaccga gagcgccgag gtcctcgacg ccggtggcga gcaccgcctc
     2281 gtaagcggcg acggcctgct cgatgtgacc ggcctgctcg aggatgctgc cgatgtgggt
     2341 ctggatcccg cggcgctcgt cgtcgctgtg cgtcagcgac agcttggtgc gcaggatgtt
     2401 gagcaggtcc tcctcgcgac cgagagcgag gtacaggcgc tcgagcgagg tgagggccgt
     2461 cggctcctcg ccgttgatct cgaggatcgc ctggttggcg gcgagggcgg cgtcgaggtc
     2521 gctgacgcgc ttctcgtaga tctccgcgag cttgaggcgc agcgggatgc tctcgggatc
     2581 gctgccgtac ttctcgatgc cggcgcgcag cgactcgacg agctcgcccc acgcaccgat
     2641 ctcctcgccg agccggacca tgtggtcgaa gatgtcgctc tgcgtgtggt tctcggtgaa
     2701 ggcctggcgg tagtagccga aggccagcgc ttgatcgtgg gcgacgcgct cggcgaggtc
     2761 ggcgaggcga cgcagcagca agttgcgctg ctcgggatcc tcgctgtgat tgagctggat
     2821 cttgaggggc acggagatgt gccgctcgtc gtgcgcctcc tcgtagaggg caaacagcgc
     2881 gttggcgacg gtcaggttgt tgtcgtcgaa gctgagcgcc ttctcgaggg cgcgggtcgc
     2941 cttgtcggcc ttctcgaggc gggtgtggta gaggtcggcg atcttgagca gcagcgtggt
     3001 gcgctgggcg ccctcggcgg tctccgactc cttctcgagc acgcggacga actcgcccca
     3061 cttgtcctgc ttggcgtaga actcctgcag ggagtccatg tcgccgacga ccaggtagag
     3121 cttcttgagg gcgtcctggg cgcgacggtt ctcggggtcg aggcggtaca gcgtctccca
     3181 cgcggtgatc gccgacttct gatcgtcgag cttgtcgctg tagagcgagc cgagcttgat
     3241 gaggtgctgg acgcgcttgg cgttgtcctt ctcgacctcg gtgagcgtgt tgaggacgcg
     3301 ggacagctgg gcccactcct tctcgcgctc gagcagcccc tcgagctgct cgagggcctc
     3361 gtggttgccg gggtcggtgg cgagcacctg gttccacagc tcgatcgaga gcgggttctt
     3421 cttgatcttg gccaccgccg tgcgggcgat gtccagcagc tgctccatgc gctgggccgg
     3481 gtccgcgagg agcgtgagct ccttctgctg gacgtggacc atcttctccc agtcacgacg
     3541 cttggcgtag aggtccttga gcttgctgat cgcctcggcg ttgtactcgt cggcctccag
     3601 cacgctctcg aagctcttga tcgcctcggc ttgattgttg aacttgtcga ggaacaggcg
     3661 gccggcctcg aggtgcagcg cggtgcggcc ggcggggtcc tgggtgagct cggcgcgacg
     3721 gcggatgcga ccgatgaggt cgggccagcg cttcatcgtg tcgaactggg cctgctgggt
     3781 ctcgacgacc ttgagcagag cctcggtgtc gccggagtcc tcgagcacct tctcgagcgc
     3841 agcgagcgtc gtgacgacga ggccgggctg gcgcaggtgg tcgcggtaga tctcgatcag
     3901 ctgccagtag gcggccttgg tgtactcggg gtcgcgcgtc gggtcctgct tgagctcctc
     3961 cttcagcaag tcggcgacgt tgctccactt gcccgactcg gcatagagct cgcgcaggcg
     4021 cgtacgcggg taacgcttgc ccgggagctt ctgaacggcg tcacgccagc cgtcgatcgc
     4081 cttggtgcca ccggcggcct cggccgtgcg ggcggcgttg aggaagcgct gctcctccgg
     4141 agagaagacc tcgccgccga tctcgatcgc ctcgccgggg atcgctgcgg cctccgccgc
     4201 ggcacgctcc gctgcggcgc gctccgctgc ggccttgtcg gcggcagcct tgtcggcagc
     4261 ggccttcttg tcggtagcgg ccttgtcggc ggcggccttg tcggcagcag ccttgtcggc
     4321 ggcagcacgc tcggacgcgg ccttgtcggc ggcagccttg tcggccgcgg cacgctcgga
     4381 cgcggcgcgc tcgctggcgg cacgctcggc ctgctcggcc gcagcacgct cggccgcggc
     4441 acgctcggca gcggcacgct cggcgacgcc gtcgtcagcc tgggcggcct cacgcgcggc
     4501 acgctcggac gcctcgcgag cgctgcgctc ggacgcctca cgggccgcgc gctcgctctc
     4561 ctcgcgagcg ccacgctcgg acgcctcacg ggccgcgcgc tcgctcgcct cacgctcggc
     4621 gcgttcggtc gcggcgcgct cgctggcggc gcgctcctcg gcggcgcggc tggcagcggc
     4681 gcgctcctcg tcggcgcgct gggcagcggc ttgctgctcg ctggtgtcgg cgatggcgat
     4741 cgggccatgg ctcgcggtga actcgcggag gatcgggtgg ttggcggcga cgctggccgc
     4801 ccagccgagc aggctgcggg ccgtctcgac gtcgttgcgg agcttccacg cgatctcgcc
     4861 ggacgtgatg gcgaccagcg ccgcgtcgat cggatcggag atcactgaca ggccgcgcgc
     4921 gccgagctcg acgaggccct cggcgctgcc ggtgcgctcg gcggtcttct tgagcaggta
     4981 gaaggcgccg aggtgccagt caccaccctg cgggtcgcgc agcgtctcgc cgccgatcga
     5041 gtacgcgtac tcgagggcct ggcgatagaa gtaactcgcc atgtcggcgt tgttgaggcg
     5101 cacctgccag atgagggcga agtcgcggag gatcgcgacc ttgtcggccg gctcctggat
     5161 cagcgcgacg cggcggtctt gcagcttctg gatgtgctgc aggtgcccgc cggcggcgag
     5221 catcgtctcg gcgacgtagg cggcctcgtg gttgagcggg ttggcgtcga gcgccttgaa
     5281 cagcagcggc agcaggcgct ggtcgtccgg agcctcctgc tgaagaatcc gagcggcgcg
     5341 catgaacagc gtcgacagcg tgtgcccggc gttctcgaca ttcgcggcga gcgggtcgct
     5401 ctgggcggtc agcgcggcga gctggttgta gatgttctgc agcgcgaact gccagttgcc
     5461 gcggtcgtac agggtctcct ggaagcggtc ctggtactcc gtgttgcggc tctcggcgct
     5521 cgcggcggcc tcgaggtagg gcctcgcgtt gtcgacctga cgcaggttga gcagggcccg
     5581 accgtatgcg tagttgagga tcggcgcacg cgcggggtcc cggttggcgc gcagctcgag
     5641 ggccatcagc ttgatgacca tctcgaggtt cgccatctgc tgatagatgg agcgagcctg
     5701 ctgcagcgcg aggaggttct cacggtcgcg cttgacggcc gtctggtaga acgtcatcgc
     5761 cctggccttg tcgaggaaga tgccctcaca agcctgagcg agctggaaaa acgcggcgga
     5821 ctgcgcctgg gccgatcctg cggaactggc ctgggactcg aattcgtcga tgaccccgtt
     5881 ccactcggtg acctcggtga gccgtgactt gatgcgcgtc gttacttcca tctgggaccc
     5941 tatttttgag cgttctcggg gaattacccc agctcgggag accgcggata taccacgctc
     6001 cgcggcggcg cttcaacgcg tccagcctgc cggggggatg aggacgaacc cggccccgca
     6061 cgccgcagag cgtcggagag gccggggaga tcggtgtagg tgaacagatg tgcccgggac
     6121 gggaggcgat ccacgaggcc atctgcggtg gatgcagcgc tggcggcgaa acaacgggag
     6181 aagccgcggc gaaaacgaca gcgagtcgcg agacgaggcg ggggcgtgaa cgccccctcg
     6241 agcacacgcg accgcgggga gtgggtcgcc ggccaacagc gttttttgag gctcggcgtg
     6301 gccgtgtccg cggttagatg acgtccgcca tccgggcgac ggtgtcgcgc gctgccttga
     6361 ccaggtccgg gcgcgtctct tgaccagcct tggagatgaa gagctcgagg ttctccttgg
     6421 cctccttgcg cttttgcagc tcggcgtagg ccatcccgag gccgtagagc gcgtcgacgc
     6481 cgtcggggtc gatcgccttg gccttcgagt aggcgtcgat cgcctcctgg aactgcccga
     6541 gctcgaagtg ggcgcggccg aggccgttcc acatgcgggc atccttgtcg ttgatctgcg
     6601 cgccggtctg aagcacgacc atcgccacgt tggcgaagcc gtagtcgatg tacatgttgc
     6661 cgagcgagac gaaggccggg ctgtagctcg gcttgatctc gatggtcttg cggtacgcgg
     6721 cgtcggcctt ctcgggctcg tcgagcttct cgtgcagcgt gccggtccga tagtgcgcct
     6781 tgtagacctt ggggtccagc ttgagcgcct cctggaacga gttgatcgcg gcgcggaact
     6841 tggtctcgcg ctccgaggag gcgacgccgt cggcctcgcc ctgggagctc tggatggcgc
     6901 cgaggtcgta gtagtactcg gcgttgggct ccgacttcat gttgtcgatc gcgccctgaa
     6961 aggccttctc ggcgtcgacg agcttgttct gcttcttgta gatctgaccg agcgtgtgat
     7021 acgcggcggc gtaggtcgga tcgagctgga tcgcgtcctg cagggccttt tcggcgcccg
     7081 gggagttgtt cttgtcgaac agctgaatgc cctcgttcat ccgattgatc gcctgcacgc
     7141 gattacggct cgccaggttg cagccgggca gaacgatgac gaaggagacg aggagtgcgg
     7201 cgatgagcgg aagacgacgc atcagagacc tttccagaac gtgcgggatg ataacggggg
     7261 gggcttctgg aaacaagacc gtgacgaaca ccgggatgaa tcggtgtctg gagccgctgt
     7321 ggggttcatg gcgggtttta cccaggcggg agcgcggcct cgcggggtcc gctgggtaga
     7381 tcgttccgag atctccacca aaagcgagcc gcggcccgca gcgtggtcca acccggcgaa
     7441 ggaccagcgg aaccaacgcc ggaagcccgg cgagggccgg gcttcaggtc gaacgaacga
     7501 gttgtcgagc gggtttatga tcgggctgga tcagcccggc tcgagcacga aggggtaggt
     7561 cacgatgacg ctgccgccgc cctcgggctt ggggaaggtc cagcgcttga cggcctgcgc
     7621 gatgcagttg ccgacgccgg cgtccttcat ggtggtctcc tggacgacgg
//
LOCUS       DASWNJ010001913         3970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_138538, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001913 DASWNJ010000000
VERSION     DASWNJ010001913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138538"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VGB85_31895"
     CDS             <1..829
                     /locus_tag="VGB85_31895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138746.1"
                     /translation="KWAKKLFTEARAAHDAEDYYNSVIKFEQAYNFAPDKHIFAFNIG
                     QDAWELKDCARVKQYLELFLIKETANQELRTKAQDLLAKAENNKDCLTGGPAPTTSGG
                     GGGGGGGASRPPEETEDPLALRKRGGDDGDDDEGPKKRGASGLLIGGVVLTVLGLGAA
                     GGGIGTMLAAKGKKDDIEGQADADGPTTFPQGRYDSGVEGDIKSLKTLNTVTPLLLGI
                     GGAMFVGGIVMIVIDRGNKKKGKGFYARKPGHGVELVGVSAAPLPGGGAAGSFALRF"
     gene            1026..2495
                     /locus_tag="VGB85_31900"
     CDS             1026..2495
                     /locus_tag="VGB85_31900"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0138747.1"
                     /translation="MSSLGFQTLYRILHELGIGAHRAFLPDAWEPMALTWPQPRRPIL
                     TYEAERPLSSYRVVGLSMAYELEIAGVIRLLEGAGIPLLAADRREHDPIVIAGGPLTF
                     SNPSILLPFIDVLLAGECEELLPAALEAILQTRGRQAAISAAAALEHACLGEVEPGLW
                     SALPEPAKAPLERLPARSVILTPDTELADMFLVEPERGCSRKCSFCVMRVSTNGGMRL
                     LPMATPLGLVPDAARKVGLVGAAVTDHPELEDIVAALVDSGRSVSISSLRADRLTPRF
                     LHDMQRSGHRTITVASDGISERMRLLLQRKIKEEHLIKAAGLIKEHGFHRMKIYEMMG
                     APGETDADADELVRLALEIAKIQRLVLTFSTFVAKRNTPLDGQPFMGVKPAEERLRRI
                     REGLRGKVETRPQSPRWAHIEYFLAQGGPEAGHAAIKAVHAGARYADWVRAFAEAPPS
                     RRELKFGDEAAWARRKGKAALPATGRPGLPGRLPILGRE"
     gene            2562..>3970
                     /locus_tag="VGB85_31905"
     CDS             2562..>3970
                     /locus_tag="VGB85_31905"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY0138748.1"
                     /translation="MSLTATILALRTVAGLSRVEPGEGEVTLSATELTTVETHWSETG
                     IRVRPWVPSKRVATLARGARLLVRGEVASRDNNGCHGKPWYAVFPFGFVCSEHVKRSK
                     AAPATGKALPLTRDRRLPYDYVITRDDGLAMYASIEDIGLGKVARTLASGSNLAVARS
                     VDVAGTPYIETVDGMLVQKQGLGWLGQGSDWSGVLLGSTGAEPVPGPAFAWTFKEKTP
                     VYAEPDRTSTQVGVLPLRARVALLEESGEEEATRFWQIAEGQWVAASQLAEVMIIEPP
                     DGVLRDNRVRSGNDQWIDVNVGEQVLVAYRDRTPVYATMISSGKGSPTPRGNYPIWAK
                     VASMTMANQDYEDKPYMVEHVPWVLLFQGHNALHGAYWHDRFGQRRSHGCVNLAPRDA
                     RWIFEWVSPTLPAQWTGYIPSDLERSVVVHVRDSSLPAAEAFIQERPIGPPDPEEEKR
                     KLELAEERRAEAALEAASAA"
BASE COUNT          597 a         1517 c         1311 g          545 t
ORIGIN      
        1 caagtgggcc aagaagctgt tcaccgaggc ccgcgcggcg cacgatgccg aggactatta
       61 caactcggtc atcaagttcg agcaggccta caacttcgct ccggacaagc acatcttcgc
      121 cttcaacatc ggccaggacg cgtgggagct caaggactgc gcccgcgtga agcagtacct
      181 cgagctgttc ctcatcaagg agacggccaa ccaggagctg cgcacgaagg cccaggacct
      241 gctcgccaag gccgagaaca acaaggactg tctcaccggc ggcccggccc cgacgaccag
      301 cggcggcggc ggcggtggtg gcggcggtgc ctcgcgcccg cccgaggaaa ccgaggaccc
      361 gctcgccctg cgcaagcgtg gcggcgatga cggcgacgac gacgagggcc ccaagaagcg
      421 cggcgcctcc ggcctgctca tcggtggcgt cgtgttgacg gtgctcggcc tcggcgcggc
      481 cggcggtggc atcggcacga tgctcgcggc caagggcaag aaggacgaca tcgagggcca
      541 ggccgacgcc gacggcccga cgaccttccc gcagggtcgc tacgacagcg gcgtcgaggg
      601 cgacatcaag tccctcaaga ccctcaacac cgtcaccccg ctgctgctcg gcatcggcgg
      661 cgcgatgttc gtcggcggca tcgtgatgat cgtcatcgac cgcggcaaca agaagaaggg
      721 caagggcttc tacgcccgta agcccggtca cggcgtcgaa ctcgtcggcg tcagcgcggc
      781 acccctgccc ggcggcggcg cagcaggctc gttcgcgcta cgcttctgac ctgatgcgtc
      841 cagccgctcc ctcgagcgct cgcagtcccc ggaaccaaag cactcgcggt cacgaccgcg
      901 gtggcccccc gccggacgac gagcgctctc tcattcgttc tcgcctctcg cgcgaggtcg
      961 gcacgctcgg ccagggccgg ccctccgagg tcgcgctcgc ctacccgagc ccgtaccgcg
     1021 cgggcatgtc gagcctcggc ttccagacgc tctaccgcat cttgcacgag ctcggcatcg
     1081 gcgcgcatcg ggcctttttg cccgacgcgt gggagccgat ggcgctgacc tggccgcaac
     1141 cgcggcgccc gatcctcacc tacgaggccg agcgcccgct gtcgagctac cgcgtcgtcg
     1201 gcctgtcgat ggcctacgag ctcgagatcg ccggcgtcat ccgcctgctc gagggcgccg
     1261 gcatcccgct gctggccgcc gaccgccgcg agcacgaccc gatcgtgatc gccggcggcc
     1321 cgctcacctt ctcgaacccc agcatcctgc tgcccttcat cgacgtgctg ctcgccggcg
     1381 agtgcgagga gctgctgccc gccgcgctcg aggcgatcct tcagacccgc ggacggcaag
     1441 ccgccatctc ggccgcggca gcgctcgagc acgcgtgcct cggcgaggtc gagcccggcc
     1501 tgtggtcggc cctgcccgag cccgccaagg ccccgctcga gcgcctcccg gcccgctccg
     1561 tgatcctgac gccggacacc gagctcgccg acatgttcct cgttgaaccc gagcgcggct
     1621 gctcgcgcaa gtgttccttc tgcgtcatgc gcgtctcgac caacggaggc atgcgactgc
     1681 tcccgatggc gacgccgctc gggctcgtgc cggacgccgc gcgcaaggtc ggcctcgtcg
     1741 gcgccgccgt caccgatcat cccgagctcg aggacatcgt cgccgcgctg gtcgacagcg
     1801 gtcgcagcgt cagcatctcg agcctgcgcg ccgatcgcct gaccccccgg tttttgcacg
     1861 acatgcagcg cagcggtcac cggacgatca ccgtcgcctc cgacggcatc agcgagcgca
     1921 tgcgcctgct gctccagcgc aagatcaagg aggagcacct gatcaaggcc gccgggctga
     1981 tcaaggagca cggttttcac cgcatgaaga tctacgagat gatgggcgcg cccggtgaga
     2041 ccgacgccga cgccgacgag ctcgtgcgcc tcgccctcga gatcgccaag atccagcggc
     2101 tcgtgctcac cttctcgacc ttcgtcgcca agcgcaacac gccgctcgat ggtcagccct
     2161 tcatgggggt caagcccgcc gaggagcgcc tgcgaaggat ccgcgagggc ctgcgcggca
     2221 aggtcgagac ccgaccgcag tcgcctcgct gggcccacat cgagtacttc ctggcccagg
     2281 gcggcccgga ggccggccac gccgccatca aggccgtgca cgccggcgcc cgctacgccg
     2341 actgggtccg cgccttcgcc gaggccccac cgtcgcgacg cgagctcaag ttcggcgacg
     2401 aggccgcctg ggcccgccgc aagggcaagg cggcgctgcc cgccaccggc cgccccggcc
     2461 tcccgggccg cctcccgatc ctcggccgcg agtagctccg cggaatgcac tccggcgccg
     2521 gcgcgttgcg gcggcgcccc tgtccttgtt aggttcccgc cgtgtcgctg actgcaacga
     2581 tcctcgcgct tcgcaccgtc gctggcctgt cccgcgtcga gcccggcgag ggcgaggtca
     2641 ccctctccgc caccgagctg accaccgtcg agacccactg gtccgagacc ggcatccgcg
     2701 tgcgcccctg ggtcccgagc aagcgcgtgg cgacgctcgc ccgcggcgct cgcctgctgg
     2761 tccgcggcga ggtcgccagc cgcgacaaca acggctgcca cggcaagccg tggtacgccg
     2821 tgttcccatt tggcttcgtg tgctcggagc acgtcaagcg cagcaaggcc gcgccggcga
     2881 ccggcaaggc cctgccgctg actcgcgatc gccggctgcc ctacgactac gtgatcacgc
     2941 gtgacgacgg cctggcgatg tacgcgtcga tcgaggacat cgggctcggc aaggtcgcgc
     3001 gcacgctcgc cagcgggtcc aacctcgccg tcgcgcgcag cgtcgacgtc gccggcaccc
     3061 cgtacatcga gaccgtcgac ggcatgctcg tgcagaagca gggcctcggc tggctcggtc
     3121 agggctccga ctggtccggc gtgctgctcg gctcgaccgg cgccgagccg gtccccggcc
     3181 ccgcgttcgc gtggaccttc aaggagaaga cccccgttta cgccgagccc gaccgcacgt
     3241 cgacgcaggt cggcgtgctg ccgctgcgcg cccgcgtcgc gttgctcgag gagtccggcg
     3301 aggaggaggc gacgcgcttc tggcagatcg ccgagggcca gtgggtcgcc gccagccagc
     3361 tcgccgaggt gatgatcatc gagccgcccg acggcgtgct gcgcgacaac cgggtccgca
     3421 gcggcaacga tcagtggatc gacgtcaacg tcggcgagca ggtgctcgtc gcctaccgcg
     3481 accgcacgcc cgtctacgcg acgatgatct cctcgggcaa gggctcgccg accccgcgcg
     3541 gcaactatcc gatctgggcc aaggtcgcca gcatgacgat ggccaaccag gactacgagg
     3601 acaagcccta catggtcgag cacgtcccgt gggtgctgct gttccagggt cacaacgcgc
     3661 tccacggcgc ctactggcac gaccgcttcg gccagcgccg cagccacggc tgcgtcaacc
     3721 tggcgccccg cgacgcgcgc tggatcttcg agtgggtctc gccgacgctg cccgcccagt
     3781 ggaccggcta catccccagc gacctcgagc gcagcgtcgt cgtccacgtc cgcgactcga
     3841 gcctgcccgc cgccgaggcc ttcattcaag agcgtccgat cggcccgccc gaccccgagg
     3901 aggagaagcg caagctcgag ctcgccgagg agcgccgggc cgaggcggcc ctcgaggccg
     3961 cctccgcggc
//
LOCUS       DASWNJ010001914         1603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_138541, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001914 DASWNJ010000000
VERSION     DASWNJ010001914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138541"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /gene="smpB"
                     /locus_tag="VGB85_31910"
     CDS             <1..440
                     /gene="smpB"
                     /locus_tag="VGB85_31910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEY0138749.1"
                     /translation="VQHRKALFEYEVLERHEAGIALMGSEVKSIRDGKCSLAEAYCQF
                     NGSGELFLFNAHIHEYPMAHSRNHLPLRQRKLLMHRKELDHLAEAVSKTGLTLIPLDM
                     HARAGKIKVAMALCRGKKTYDKRAAIKEREQKREISRAIREHK"
     gene            437..1258
                     /locus_tag="VGB85_31915"
     CDS             437..1258
                     /locus_tag="VGB85_31915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138750.1"
                     /translation="MNPPRLRLVQPGSAVPEDGRDDEAREDRGSPEPTPWRGSPEPPP
                     ASPLRLVSPPVLGDRPPPEHALAELDTLLAGPLDDPACLRLAALAAQVPGRQRAVAAA
                     LAQLASAGAVVALRGLPPAAPGVLDALLRCFSRGVGSPLHAPVPGDIPVPGEAPLLAL
                     DFRGSKARGFPDLVARGQAIAARSGAAEFAALVVDGRPHYRLTFWTGRATAAALAAQL
                     RAAHLDLAWLHGRLARLRGSRLWLGGWRFDADDPLGPAAQAHLLQAWLRHAEQGP"
     gene            1255..>1603
                     /locus_tag="VGB85_31920"
     CDS             1255..>1603
                     /locus_tag="VGB85_31920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138751.1"
                     /translation="MTRTALAGPARSLRAGVRALLLRSDDEARAIHLLEELGAALGWP
                     VHTWSVTSGADGKGAPVPLAALLRELAGRREPALWLLLDPPGDPLAARALRELAQQRG
                     PGAAAVLIGEPAVI"
BASE COUNT          193 a          656 c          555 g          199 t
ORIGIN      
        1 tcgtgcagca ccgcaaggcg ctgttcgagt acgaggtgct cgagcgtcac gaggcgggca
       61 tcgccctcat gggcagcgag gtcaagtcga tccgcgacgg caagtgcagc ctcgccgagg
      121 cgtactgcca gttcaacggc agcggcgagc tgttcctctt caacgcgcac atccacgagt
      181 acccgatggc gcactcgcgc aatcacctcc cgctgcgcca gcgcaagctg ctgatgcacc
      241 gcaaggagct cgaccacctc gccgaggcgg tctcgaagac aggcctgacc ctgatcccgc
      301 tcgacatgca cgcgcgcgcc ggcaagatca aggtcgctat ggcgctgtgc cgcggcaaga
      361 agacctacga caagcgagcg gcgatcaagg agcgcgagca gaagcgggag atcagccggg
      421 cgatccggga gcacaagtga acccaccccg gctgcgcctg gtgcagcccg ggtcggctgt
      481 cccggaggac ggccgggacg acgaggcacg cgaggaccgg ggatcccccg aacccacgcc
      541 ttggcgggga tcccccgaac ctcctcctgc gtccccgctg cgcctggtct cgccgcctgt
      601 cctcggggac aggccgccgc ccgagcacgc gctcgccgag ctcgacacct tgctcgccgg
      661 cccgctcgac gaccccgcgt gcctgcggct cgccgcgctc gcggcccagg tccccggccg
      721 tcagcgcgcc gtcgcggccg cgctcgcgca gctcgccagc gccggcgccg tcgtggcgct
      781 gcgcggcctc ccgcccgccg cccccggggt gctcgacgcg ctgctgcgct gcttctcgcg
      841 cggcgtcggc tcgccgctgc acgcacctgt ccccggggac atccctgtcc ccggggaggc
      901 cccgctgctc gcgctcgact ttcgcggctc gaaggcccgc ggcttccccg acctcgtcgc
      961 gcgcggccag gcgatcgccg cgcgcagcgg cgccgccgaa ttcgccgcgc tcgtcgtcga
     1021 cggccgcccg cactatcgcc tgaccttctg gacaggtcgg gccaccgcgg ccgccctcgc
     1081 cgcccagctg cgcgccgccc acctcgacct cgcgtggctg cacggccgcc tcgcccgcct
     1141 gcgtggcagc cggctatggc tcggcggctg gcgcttcgac gccgacgacc cgctcggccc
     1201 ggccgcgcaa gcccacctgc tccaggcctg gttgcggcac gcggagcagg gcccgtgacc
     1261 cgcaccgcgc tcgcgggccc ggcccgcagc ctgcgcgccg gcgtgcgcgc cctgctgctg
     1321 cgcagcgacg acgaggcccg cgcgatccat ttattggagg agctgggcgc cgcgctcggc
     1381 tggccggtgc acacctggag cgtcaccagc ggggccgatg gcaagggcgc cccggtcccg
     1441 ctcgccgcgc ttctgcgcga gctcgccggt cgccgcgagc cggcgctgtg gctgctgctc
     1501 gacccgcccg gcgacccgct cgccgcccgc gcgctgcgcg agctggccca gcagcgcgga
     1561 cccggggccg ccgcggtgct gatcggcgag cccgcggtga tct
//
LOCUS       DASWNJ010001915         3203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_599045, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001915 DASWNJ010000000
VERSION     DASWNJ010001915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599045"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VGB85_31925"
     CDS             complement(<1..694)
                     /locus_tag="VGB85_31925"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0138752.1"
                     /translation="MHVRRLVLVPLLLSLACERQTGTQPPKTADPATTPALAAPPPLQ
                     RPSLVARDPIGAGVLAALDTTVDPCTDFYRYACGGWLKKTTRPEDKPRFGRGFGELAD
                     RNNAVLRKILEDAAAIKGKQGPVEAKLGAFWTACTDEATIDRAGITPLRPVLAQVDAV
                     KDEASLMRVVGKLHATYFGGGGPLFSFYVEPDAKRPEVYVAQAAQGGTGLPDRDFYLR
                     TDPQSKGLLTAYE"
     gene            complement(793..2103)
                     /locus_tag="VGB85_31930"
     CDS             complement(793..2103)
                     /locus_tag="VGB85_31930"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEY0138753.1"
                     /translation="MSLVDLSPEAFASHLLSLGVRRAYAVFDARTQRVEVSHPELGEL
                     REFLGAGNRDFDQHEGVFLEVGEQSGALFGAFVHRSNRGQGQGGLRHWPYGTLAAFLR
                     DGLRLARGMTRKNALAGLWWGGGKGLIARVPGEGYRDLNTRATLYSEYARFVTSLRGC
                     YITAEDAGTGPEDMAVVHQFTRFVTCVPPSIGGSGNPSPATARGVVSAMEGALDHLGL
                     GTLAGKKVVMQGTGNVATAMIGELLQRKVGAIVASELSLERRAALSQQFAGAPVALVP
                     AQAGDNSILAEPCDILAPNALGGILNPDTIPAIQARIVCGAANNQLLDDVRDAQALAD
                     RGIVYVPDYLCNRMGIVNCANEQYGQVPDDPAITRHFGRDWDNSVYVITRRALELAQQ
                     QNITTAAAANRLADELARVPHPIWGHRTQAIIDGLIADRWHLGG"
     gene            complement(2190..>3203)
                     /locus_tag="VGB85_31935"
     CDS             complement(2190..>3203)
                     /locus_tag="VGB85_31935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138754.1"
                     /translation="SSSVLAAPAPVQAPAPVTTPAPARPPATGLTPLTAEQRATLLAG
                     PEDTLAETPIHYVKSNEVRHDVWFPYVRGVGGAYLGVGSDQNYTIAAAARSELMFLSD
                     IDRSVVDLHRIYEILIESSDTPEALHARFDRAEEETSAALLDSGFAALPEAERRRLVR
                     LYRNARETVYRHLRHVIKRKRNGEPSSWLSDPVMYDHIRALFQADRVRQMGGDLTGSN
                     SLQTAAAAARALGVPFRVIYFSNAEEYFDYSKSFIANIQAFETDERSVLLRTIYAKDW
                     VHADLLWAYQVQPLADFKRRLGERKNRSRNPMFKYAELDGTLNREPGTKGLSLIALPV
                     AAG"
BASE COUNT          444 a         1107 c         1185 g          467 t
ORIGIN      
        1 gctcgtaggc cgtcaacaga cccttcgact gcggatccgt gcgcagataa aagtcgcggt
       61 cgggcaagcc ggtgccgccc tgggccgcct gcgcgacgta gacctccggg cgcttggcgt
      121 cgggctcgac gtagaagcta aacagcgggc caccgccgcc gaagtaggtc gcgtgcagct
      181 tgccgacgac gcgcatcagg ctcgcctcgt ccttgaccgc gtcgacctgc gcgagcacgg
      241 gccgcagcgg cgtgatgccg gccctgtcga tcgtcgcctc gtccgtgcac gcggtccaaa
      301 acgcgccgag cttggcctcg accggcccct gcttgccctt gatcgccgcg gcgtcctcga
      361 ggatcttgcg cagcaccgcg ttgttgcggt ccgcgagctc gccgaagccg cggccgaagc
      421 gaggcttgtc ctccgggcgc gtcgtcttct tgagccagcc gccgcacgcg tagcgataga
      481 agtcggtgca ggggtcgacg gtggtgtcga gcgccgcgag aacacccgcg ccgatcgggt
      541 cgcgggcgac cagcgagggc cgctgcagcg gcggcggagc ggccagcgcc ggggtcgtcg
      601 ccgggtccgc ggtcttcggc ggctgggtgc ccgtctggcg ctcacaggcg agagacagca
      661 ggagagggac gaggacgagg cggcgcacgt gcatcgcggg gaccatgcca tacgcagtga
      721 gctccgcgcg gccgcgcccc cggccccgca gcggaggaaa ttcacgcgcg aagcgccgcg
      781 ttggcccgaa actcagccac cgaggtgcca gcggtcggcg atcaggccgt cgatgatcgc
      841 ctgcgtgcga tgaccccaga tcgggtgcgg cacgcgggcg agctcgtcgg cgaggcggtt
      901 ggccgccgcc gcggtcgtga tgttctgctg ctgcgccagc tcgagcgccc gccgcgtgat
      961 cacgtagacg ctgttgtccc agtcgcgccc gaagtgccgc gtgatcgccg ggtcgtcggg
     1021 cacctggccg tactgctcgt tggcgcaatt gacgatgccc atgcgattgc acaggtagtc
     1081 cgggacgtac acgatcccgc ggtcggcgag cgcctgcgcg tcgcggacgt cgtcgagcaa
     1141 ttgattgttg gccgcgccgc acacgatgcg cgcctggatc gcggggatcg tgtccgggtt
     1201 gaggatcccg ccgagagcgt tgggcgccag gatgtcgcag ggctccgcga ggatgctgtt
     1261 gtcgcccgcc tgcgcgggga ccagcgcgac cggggcgccg gcgaattgct gcgacagcgc
     1321 ggcgcgccgc tcgagcgaca gctcgctcgc gacgatcgca cccactttgc gctgcagcag
     1381 ctcgccgatc atcgcggtgg cgacgttgcc ggtgccctgc atgacgacct tcttgcccgc
     1441 gagcgtgccg aggccgaggt ggtcgagcgc gccctccatc gcgctgacga ccccgcgagc
     1501 ggtcgccggc gacgggttgc ccgagccgcc gatgctcggc ggcacgcagg tgacgaagcg
     1561 cgtgaactga tgcacgaccg ccatgtcctc gggcccggtg ccggcgtcct cggcggtgat
     1621 gtagcagccg cgcaggctgg tcacgaagcg cgcgtactcg ctgtacagcg tcgcgcgggt
     1681 gttcagatcg cggtagccct cgccgggcac gcgcgcgatc agccccttgc cgccgcccca
     1741 ccacagcccg gcgagcgcgt tcttgcgggt catgccccgc gcgagccgca gcccgtcgcg
     1801 caggaacgcc gcgagcgtgc cgtacggcca gtgccgcagg ccgccctgac cctggccgcg
     1861 gttgctgcgg tgcacgaagg ccccgaacag cgcgccgctc tgctcgccga cctcgaggaa
     1921 cacgccctcg tgctggtcga agtcgcggtt gccggcgccg aggaactcgc gcagctcgcc
     1981 gagctccggg tgcgagacct cgacccgctg cgtgcgcgcg tcgaagacgg cataagcccg
     2041 gcgaaccccg agcgagagca gatgcgaggc gaaggcttcc gggctgaggt cgaccaggct
     2101 catggccggc gcaggttaac cgaagccagc cgcgtgcgta ccgtgagagc gcacacgcat
     2161 cgaccgcgca aaccggttcg ccaggcgttt cacccggccg cgaccggcag cgcgatcagg
     2221 ctcaggccct tggtgccggg ctcgcggttg agcgtgccgt cgagctccgc gtacttgaac
     2281 atcgggttgc gcgagcggtt cttgcgctcg ccgaggcgac gcttgaagtc ggcgagcggc
     2341 tggacctggt aggcccacag caggtcggcg tgcacccagt ccttggcgta gatcgtgcgc
     2401 agcaggacgc tgcgctcgtc ggtctcgaag gcctggatgt tggcgatgaa cgacttcgag
     2461 tagtcgaagt actcctcggc gttgctgaag tagatcacgc gaaacggcac cccgagcgcg
     2521 cgcgcggccg cggccgcggt ctgcaacgaa ttgctgccgg tcaggtcgcc gcccatctgg
     2581 cgcacgcggt cggcctggaa cagcgcgcgg atgtggtcgt acatcaccgg gtccgacagc
     2641 cacgacgacg gctcgccgtt gcgcttgcgc ttgatgacgt gacgcaggtg cctgtacacc
     2701 gtctcgcggg cgttgcggta caggcggacc aggcggcggc gctcggcctc cggcagcgcc
     2761 gcgaagccgc tgtcgagcag cgccgcgctc gtctcctcct cggcgcggtc gaagcgggcg
     2821 tgcagcgcct cgggggtgtc ggacgactcg atcaggatct cgtagatgcg gtgcaggtcg
     2881 acgacgctgc ggtcgatgtc cgacaggaac atcagctcgc tgcgcgccgc cgcggcgatc
     2941 gtgtagttct gatcggagcc gaccccgagg tacgcaccgc cgacgccgcg gacgtacggg
     3001 aaccacacgt cgtggcggac ctcgttgctc ttgacgtagt ggatcggcgt ctcggcgagc
     3061 gtgtcctccg ggccggccag cagcgtcgcg cgctgctcgg ccgtcagcgg cgtcaggccg
     3121 gtggcggggg gcctggccgg cgcgggcgtg gtcaccggcg cgggcgcctg gaccggggcc
     3181 ggcgcggcga gcacggagga gct
//
LOCUS       DASWNJ010001916         6608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_599054, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001916 DASWNJ010000000
VERSION     DASWNJ010001916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6608
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599054"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VGB85_31940"
     CDS             complement(<1..451)
                     /locus_tag="VGB85_31940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138755.1"
                     /translation="MRTRSLATALLLAACSGGTNDTDDTGSASTTGTTDTTGADDPDR
                     ETLVHSFGVRTMAPFAESEPCAQWTLDNEREIYVNTVTLVNDGGFHHSNWLAVPEERF
                     AGPDGFFDCEERHYTELDGAVSGTVLFAQSTQSRREVQQLPPGVVVKI"
     gene            complement(515..949)
                     /locus_tag="VGB85_31945"
     CDS             complement(515..949)
                     /locus_tag="VGB85_31945"
                     /inference="COORDINATES: protein motif:HMM:NF012843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HEY0138756.1"
                     /translation="MTDEPLPERSVRTLAWLGDAEFEREVRVRLARRGDFPTDRLHAM
                     KARVVCAEAQAELLATLEPVLDEAESGVVRRARNLNVKAGGRGVRSARDYRSSTALEA
                     LVAHWCLGPTGGRQRFEALVIPWLEARIDAALVVARHAPKRG"
     gene            complement(946..3660)
                     /gene="uvrC"
                     /locus_tag="VGB85_31950"
     CDS             complement(946..3660)
                     /gene="uvrC"
                     /locus_tag="VGB85_31950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEY0138757.1"
                     /translation="MRGRRGKKPEPQAGESEVESAAENLELADAMAIDLDEPGRFGEA
                     QAAAQIAPVVDAKWQPRARGLEQINALVDRLSDDPGVYVMRDRKGVIVYIGKARRLRA
                     RVKQYFNGTDTRHFVPYLANLLGDIETVVTSSDKEALLLENNLIKKYKPRFNVKLRDD
                     KQFLVLRLDPKASWPRLELVRRMRQDGASYFGPYHSANSARHTLRVVNRYFKLRTCTD
                     YTLTHRSRPCLQHQIGRCPAPCVYPVDREAYGGQVKNVGLFLNGRHKDLVHALQGQME
                     AAAAALEFESAARLRDQLNAIETSLERQQIVDNSAIDQDVIGMYREGGQVEFVVMHVR
                     QGKLLGNRSFSARGMELPDAEVLGSFLLAYYDDAPLIPDELILPSPLVEDDEAPLKAW
                     LRETSGRKVSVVVPERGDRKKLLSLAQKNAASCFATRRNRQEDSDQALLSLKQRLGLS
                     KLPRTIECYDISHIQGSDTVASMVVFVDGAAARKRYRSFRIRGGSEGLAQGNRQNDDF
                     ASMAEVLGRRLRRGLAVAGVAKGPEQEDGEDGEVSDAWALPDLIVIDGGKGQLGRVLA
                     MMRDLGITVGAGGVDVVSLAKERKVEIGRGKSALEQLREHRAKMRSNSAKGALRVEDS
                     GIDRGAAQRYVEAARAGMAEVAARLAENGGAAPTAVAGDAASDDRGVRGGGADTGDAF
                     ATGDEDAATKSEATSAVDSGDASSAGDEDAATKPEGTSAVETGDASSAADEELTTDPE
                     GAVDFGDEPIDEANALDAVLSDDVASGEPVARPGETYQNYVLTAEEDDAADAEVRPER
                     VFVPGAKEAIRLRPGTSELFLMTQIRDEAHRFAITHHRKRRGKRALHSALDDIPGVGP
                     NLKKTLLQAFGSVAGIVAADDAALTAVKGVGPALAKRLREALGGGGPT"
     gene            complement(3793..4899)
                     /locus_tag="VGB85_31955"
     CDS             complement(3793..4899)
                     /locus_tag="VGB85_31955"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease"
                     /protein_id="HEY0138758.1"
                     /translation="MFTLLWPLLGILGVSLLIVIHEFGHFISARATGMRVDRFSVLGI
                     GPVIARLGTWKGTEFVISAIPFGAYVHIVGMEAGDEPAQTLPPIKEGDPPPTVYDPKD
                     PYLFRNRPVWARGLAILGGPLANYVTAMVLFFALFATVGHRELVSRKATADISETAAA
                     AGVQPGDEFVKIAGQDVQGRDPEVKIAAATGAHKGETVPVVMQRGDQEVTLQVPVNEE
                     GRMGIGLDRGKVITTPASWSEAAIFGVTQPYIVSKQNLQAMGKLFTGATAMSQMSGPI
                     GIVKQVAKAAEKGPGEYVMLMAIISALLGLFNLLPLPALDGGRMVFMLIEAVIGRPIS
                     KSVEETIHGYGMLALLGLILYVTIRNDLLGSFFK"
     gene            complement(4946..5533)
                     /locus_tag="VGB85_31960"
     CDS             complement(4946..5533)
                     /locus_tag="VGB85_31960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138759.1"
                     /translation="MPVQLSVILLLRDREPAVAAMVRQAAALGRSAVTEGGRFEVLAL
                     DEHSGDNTLSLLSVLHGRLPELRTIQEVREGTAVAQAARTARGEKWLFIDREVEPELL
                     RWGVRQLAGGQRAAVVPGEILAVEARLGAHVLADLYGGLVSAQQAVARELTTRGSRPA
                     TRPAPDRGLTERALLFLRGRLGMVGLGQLDRPRGT"
     gene            5906..6517
                     /gene="ssb"
                     /locus_tag="VGB85_31965"
     CDS             5906..6517
                     /gene="ssb"
                     /locus_tag="VGB85_31965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY0138760.1"
                     /translation="MAGVNKAIILGHLGRDPELRYLQNGQPVCKLNIATSRKYTNKNN
                     ESVEETEWHRVSVWGKQAEHCNNFLTKGRQVYVEGRLRTSSYDKERQKHYTTEIVADT
                     VQFIGGRGEGGEGGGGGSAPGGGGGGGYGGGGGGGGGRPAGGGGGGYGGGGARPAPGG
                     GRPAPGGGRGGGGGGGGFDEGGGAPEPDFNDFGGNPGDDDIPF"
BASE COUNT          944 a         2449 c         2183 g         1032 t
ORIGIN      
        1 ggatcttcac gaccacgccg ggtgggagct gctggacctc gcggcgcgac tgcgtcgact
       61 gggcgaacag caccgtgccc gagaccgcgc cgtcgagctc cgtgtagtga cgctcctcgc
      121 agtcgaagaa gccgtcggga ccggcgaacc tctcctccgg caccgcgagc cagttcgagt
      181 ggtggaaccc gccgtcgttg accagcgtca ccgtgttgac gtagatctcg cgctcgttat
      241 caagagtcca ctgcgcacac ggctcggact cggcgaaggg cgccatggtg cgcacgccga
      301 agctgtggac cagcgtctcg cggtccgggt cgtccgcacc tgtggtgtcg gtcgtgccgg
      361 tcgtgctcgc actgcccgta tcatccgtgt cgttcgtgcc gccgctgcac gcggcgagca
      421 atagcgctgt cgcaagcgat cgagttcgca tcaccacgct ccttgagagt gcgagcaacc
      481 tagcaaatgc gggcgcatcc gccacgcctc atcgtcatcc gcgcttcggg gcgtggcggg
      541 cgacgacgag ggcggcgtcg atgcgcgcct cgagccatgg gatcacgagc gcctcgaagc
      601 gctgcctgcc gcccgtcggg ccgaggcacc agtgcgccac cagggcctcc agcgcggtcg
      661 aggaccggta gtcccgggcg cttcgcaccc cgcggccccc cgccttgacg ttgaggttgc
      721 gcgcgcgacg gaccaccccc gactccgcct cgtcgagcac cggctccagg gtcgccagca
      781 gctccgcctg cgcctccgca cacacgaccc gggccttcat cgcgtgcagt cgatcggtcg
      841 ggaagtcgcc gcgacgcgcg agccggaccc ggacctcgcg ctcgaattcc gcgtcaccga
      901 gccacgcgag ggtgcgcacc gacctctcgg gcaagggctc gtcggtcatg tcgggccgcc
      961 gcccccgagg gcctcacgca ggcgtttggc gagcgccggg ccgacgccct tgaccgcggt
     1021 cagcgcggcg tcgtcggccg cgacgatccc cgcgaccgag ccgaacgcct gcagcagcgt
     1081 ttttttgaga tttgggccga cgcccgggat gtcgtcgagc gcgctgtgca acgcccgctt
     1141 gccgcgacgc ttgcggtggt gcgtgatcgc gaagcggtgc gcctcgtcgc ggatctgcgt
     1201 catcaggaac agctcggagg tcccggggcg cagccggatc gcctccttcg cgcccggcac
     1261 gaacacacgc tcgggccgca cctcggcgtc cgccgcgtcg tcctcctcgg cggtgagcac
     1321 gtaattttga taggtctcgc ccggacgcgc gacgggctcg ccgctcgcga catcgtcgga
     1381 cagcacggca tccagcgcat tcgcctcgtc gatgggctcg tcaccgaagt cgaccgcgcc
     1441 ctccgggtcc gtcgtcagct cctcgtccgc tgcgctcgat gcatcgccgg tttcgaccgc
     1501 gctggtgccc tccggcttcg tcgccgcatc ttcgtcccct gcgctcgacg catcgccgct
     1561 gtcgaccgcg ctggtggcct ccgacttcgt cgccgcatct tcgtcccctg tggcgaacgc
     1621 atcgccggtg tcggccccgc cccctcgcac cccacgatcg tcggacgccg catcacccgc
     1681 gaccgcggtc ggcgctgcac cgccgttctc ggccaacctc gcggcgacct ccgccatccc
     1741 ggcgcgcgcc gcctcgacat agcgctgcgc cgcgccgcgg tcgatcccgg agtcctcgac
     1801 ccgcagggcc ccctttgccg agttgctgcg catcttggcc cggtgctcac gcagctgctc
     1861 gagggccgac ttgccgcgcc cgatctcgac cttgcgctcc ttcgccagcg acaccacgtc
     1921 gacgccgccg gcaccgaccg tgatcccgag gtctcgcatc atcgcgagca cgcggccgag
     1981 ctggcccttg ccaccgtcga tgacgatcag gtccggcagc gcccatgcat cgctgacttc
     2041 cccgtcctcg ccgtcctcct gctcgggccc cttcgccact ccggcgaccg cgagcccgcg
     2101 tcgcagccgg cgcccgagca cctcggccat gctcgcgaag tcgtcgttct gccggttgcc
     2161 ctgcgcgagc ccctcggagc cgccgcggat gcgaaacgac cgataacgct tgcgcgccgc
     2221 cgcgccgtcg acgaacacca ccatcgacgc gaccgtgtcc gacccctgaa tatggctgat
     2281 gtcgtagcac tcgatcgtgc gcgggagctt gctcaacccg agccgctgct tcaacgacag
     2341 cagcgcctgg tcgctgtcct cttgccgatt tctgcgagtg gcaaagcacg acgccgcgtt
     2401 cttctgcgcc agcgacagca gcttcttgcg atcgccgcgc tcgggcacca ccacgctgac
     2461 cttgcgcccg ctcgtctcgc gcagccaggc cttgagcggc gcctcgtcgt cttccaccag
     2521 gggcgacgga aggatcagct cgtcggggat cagcggcgcg tcgtcatagt aggcgagcag
     2581 gaagctgccg agcacctccg cgtcgggcag ctccatgccg cgcgccgaga agctgcggtt
     2641 gccgagcagc ttgccctggc gcacgtgcat caccacgaac tcgacctgac cgccctcgcg
     2701 gtacatgccg atcacgtcct ggtcgatcgc gctgttgtcg acgatctgct ggcgctcgag
     2761 gctcgtctcg atcgcgttga gctggtcgcg caggcgcgcc gccgactcga actcgagcgc
     2821 cgccgccgcc gcctccattt gcccctgcaa cgcgtgcacg aggtccttgt ggcgcccatt
     2881 caaaaacagg ccgacgttct tgacctgccc gccgtacgcc tcccgatcga ccgggtacac
     2941 gcacggcgcc gggcagcgcc cgatctggtg ttgcaggcac gggcggctgc ggtgcgtgag
     3001 cgtgtagtcc gtgcaggtgc gcagcttgaa atagcggttc accacacgca gcgtgtggcg
     3061 ggccgagttc gccgagtggt acggcccgaa gtacgacgcg ccgtcttgcc gcatccggcg
     3121 gaccagctcg aggcgcggcc agctcgcctt cgggtcgagg cgcaggacca ggaactgctt
     3181 gtcgtcgcgc agcttcacgt tgaagcgcgg cttgtatttc ttaattaaat tgttctcgag
     3241 cagcagcgcc tccttgtcgc tggaggtcac caccgtctcg atgtcgccga gcaggttggc
     3301 gaggtacggc acgaagtgtc gcgtgtccgt cccattaaaa tactgcttca cgcgcgcgcg
     3361 caggcgccgg gccttgccga tgtagacgat gacgcccttg cggtcgcgca tcacgtacac
     3421 gccggggtcg tcggacaggc ggtcgacgag cgcgttgatc tgctcgagcc cgcgggcccg
     3481 cggctgccac ttcgcgtcga ccaccggcgc gatctgggcc gccgcctggg cctcgccgaa
     3541 gcgacccggc tcgtcgaggt cgatcgccat cgcgtcggcc agctcgaggt tctcggccgc
     3601 cgactcgacc tcggactcgc ccgcctgcgg ctccggcttc ttgccgcgcc ggcccctcat
     3661 cggcaccgtc ccgggcgctg gcttctcggg cgtggccgca gcaggagggt tcgttttcgc
     3721 cccctgcgga tcggtcgagg ccggcccgcc tggcgtgccg ctgcgtcgtg gtcgactcat
     3781 gcgcgctacg tattacttaa aaaagctgcc gagcaggtcg ttgcggatcg tgacgtagag
     3841 gatcaggccg agcagcgcga gcatgccgta gccgtggatc gtctcctcga cgctcttgct
     3901 gatcgggcgg ccgatcaccg cctcgatcag catgaacacc atgcgcccgc cgtccagcgc
     3961 gggcagcggc agcaggttga acaggccgag cagcgcgctg atgatcgcca tcaacatcac
     4021 gtactcgccg gggcccttct cggccgcctt ggcgacctgc ttgacgatgc cgatcgggcc
     4081 cgacatctgg ctcatcgccg tcgcgccggt gaacagcttg cccatcgcct gcaggttctg
     4141 cttcgatacg atgtacggct gcgtcacccc gaagatcgcc gcctcggacc agctcgccgg
     4201 ggtcgtgatc accttgccgc ggtcgaggcc gatgcccatg cggccctcct cgttgaccgg
     4261 cacctgcagc gtgacctcct gatcaccgcg ctgcatcacc accggcaccg tctcgccctt
     4321 gtgggccccg gtcgccgccg cgatcttgac ctcggggtcg cggccctgga cgtcctgccc
     4381 ggcgatcttc acgaactcgt cgccgggctg cacaccggcc gccgccgccg tctcgctgat
     4441 gtcggcggtc gccttgcgcg agaccagctc acggtggccg acggtcgcga acagcgcgaa
     4501 gaacagcacc atcgccgtga cgtagttggc gagcggtccg ccgaggatcg ccagcccgcg
     4561 cgcccacacc gggcggttgc ggaacaggta cgggtccttc gggtcgtaca ccgtcggcgg
     4621 cgggtcaccc tccttgatcg ggggcagcgt ctgcgccggc tcgtcgccgg cctccatgcc
     4681 gacgatgtgc acgtaagcgc cgaaggggat cgcgctgatg acgaactcgg tgcccttcca
     4741 ggtgccgagc cgggcgatca cggggccgat gccgagcacg ctgaagcggt cgacccgcat
     4801 ccccgtcgcc cgggcgctga tgaagtgccc gaactcgtgg atgacgatca ggagcgacac
     4861 cccgaggatg ccgagcaacg gccagaggag cgtgaacaac accggccgac tatagcagca
     4921 cgatccgcct gatcccggcg cagaactagg tcccgcgcgg gcggtcgagc tgcccgaggc
     4981 cgaccatgcc gagccgaccc cgcaggaaca acagcgctcg ctcggtcagc ccgcgatccg
     5041 gcgccggccg cgtcgcaggc cggctgccac gcgtagttag ctcgcgtgcg accgcctgct
     5101 gcgccgacac cagcccgcca taaagatcgg cgagcacgtg cgcgcccagc cgggcctcga
     5161 ccgcgaggat ctctcccggc accacggcag cgcgctgtcc acccgcgagc tgacgcacgc
     5221 cccagcgcag cagctccggc tcgacctcgc ggtcgatgaa cagccacttc tcgccgcgcg
     5281 cggtacgagc cgcctgcgcc accgcggtcc cctcgcgcac ctcctggatc gtccgcagct
     5341 cgggcagccg accgtgcagg accgacagca ggctcagcgt gttgtccccg gagtgctcgt
     5401 cgagcgccag gacctcgaag cggccgccct cggtcaccgc gctgcggccc agcgccgccg
     5461 cctgccgaac catggcggcc acggccggct cacggtcgcg taacaggagg atgacgctga
     5521 gctggaccgg catgcgtgcg cggcagcggg gagctcccgc agcttcttac gtcgtaagcc
     5581 ctgtcacggg gaacggtcaa gcaaacagcg tatggagtct gctcacggga gggcgcgccg
     5641 ggcacgggtg gggcctggtc gaccgtgtgg atcgtcacgg ttgtcacaca ctgtgctcgg
     5701 gcaccccggc cccgcatgag tgagatgcga gtcatctgca acgatcgcgc gtgacgtacc
     5761 gagtacgggt ccgcgcggct gagcaaattc gctatgctcc cgccgcccgc ggcggtacac
     5821 ccccgccgcg ctgcgttgcc gtccatttca tcccgggcgg cgccgcacaa catctagcgc
     5881 ccacaacgag agatccactg ctcccatggc cggcgtcaac aaagccatca tcctcggtca
     5941 cctcggccgg gaccccgaac ttcgctacct tcagaacggg caacccgtct gcaagctcaa
     6001 cattgcgacc tcgcgcaagt acaccaacaa gaacaacgag agcgtcgagg agaccgagtg
     6061 gcaccgcgtc tcggtgtggg gcaagcaggc cgagcactgc aacaacttcc tgaccaaggg
     6121 ccggcaggtc tacgtcgagg gtcgcctgcg caccagcagc tacgacaagg aacgccagaa
     6181 gcactacacc accgagatcg tcgcggacac ggtccagttc atcggcggcc gcggtgaagg
     6241 tggcgagggc ggcggcggcg gcagtgcccc gggcggcggc ggtggtggtg gctacggtgg
     6301 tggcggcggc ggcggtggtg gccgtcccgc gggcggtggt ggcggcggct acggtggtgg
     6361 cggcgcccgt cctgcacccg gcggtggtcg tcctgcacct ggcggcggcc gtggcggtgg
     6421 tggtggcggc ggcggcttcg acgagggcgg tggtgccccc gagcccgact tcaacgactt
     6481 cggcggcaac ccgggcgacg acgacatccc gttctgagcc gtcccgggcg tcgaccgccc
     6541 gcacgtatgc agtccttcgc gcccgtcgcg cggtcccgag cccggggtcg cgcgaccgcc
     6601 gttgcagg
//
LOCUS       DASWNJ010001917         4111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_173772, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001917 DASWNJ010000000
VERSION     DASWNJ010001917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173772"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VGB85_31970"
     CDS             complement(<1..697)
                     /locus_tag="VGB85_31970"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0138761.1"
                     /translation="MPVVERTIEISGLSLAARVHGPDDGMPVLAMHGWLDNAASFDGL
                     APLLSGCRVVALDLAGHGLSQRRAGAPYHFIDYVADVAAVADALGWQKFSLLGHSLGA
                     GVAALLAGTWPDRVQRLVMLEGLGPLTDPDESAPERLRESLAAELAATRRSGERPGYP
                     DPEVVGRRLAEATQMQPSSAQTLLRRGLFEALPGRWDWRADSRLRLPSRLRMTEAQVT
                     AFLKQIACPTLLLR"
     gene            829..2142
                     /locus_tag="VGB85_31975"
     CDS             829..2142
                     /locus_tag="VGB85_31975"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0138762.1"
                     /translation="MNPANEAIWIGYTTLYREGGAKFERAALTLAAEKRAAFPASVVR
                     CERVESKAEFRAAMQRIADEGLVLRELHLVVHAGMYGPMFGSVAWPEQFSPHEWRMMK
                     LPFGPGAEAFFHACRSARWFAPFFARTFGVPAHGYHWYTTVSSAPDRFRWDGLVGPEA
                     PLYIFGMPGNKSHGVPGTVRKYLGLIAPERMRRFEPAPIEGDPSYDAVAAQYDRVFSD
                     IRVRSDEWRWIDARIPAGARVLDVGCGNGALLKLLAPCITSGVGVDASRGMIDRARAR
                     TAGVPNLRFEVIDGPSLPLEDNSVDVVVSLLSFRYLDWDPIMREFARVLAPGGRLLVV
                     DMVAAPLGLRDIPGLLRDKARTLVQQLRHREYHGELARMVSDPKWQDMLRYNPIRAIH
                     ELRWYFKSRFPDGTVETLNTGWHARVLAFDTGPFEQGNVDPQSFP"
     gene            2149..2898
                     /locus_tag="VGB85_31980"
     CDS             2149..2898
                     /locus_tag="VGB85_31980"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEY0138763.1"
                     /translation="MKLGVLDWGIGGIDFYARWKARHPEVPVVYWSDSGATPYGKLGR
                     DALAQRVAGVVRALQGRGVTHVVVACNAASTVIGHPALDLGVEVCGVIGPAIAATLAD
                     PGRIVGVIGGWRTISSGLYRDGLIAGGRRVISRVAQPLSAMVERGELDSPAVHAELGR
                     ILRPLRRVEALVLACTHYPALLGPIRRVLPGVRIVDPAAAALAEVERRWCLSGQGEAD
                     MFLTTGDPQATREAARLAFGVTLPPVRRVTL"
     gene            complement(2899..3375)
                     /locus_tag="VGB85_31985"
     CDS             complement(2899..3375)
                     /locus_tag="VGB85_31985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138764.1"
                     /translation="MTRTLGVTALLWVTACGGDSKPAEVKKEEAAVEAPAKAEAPNKF
                     KAEREAKAKAAEEAAANKVKQVDALTVLPAKLPKKLAPACEELNVAMDAFMVKWFPAN
                     WPMQKTKQLADMKESCLKGTIETAACQANALAGAPQELNKAVPDLLGACIEKFGKK"
     gene            complement(3465..3794)
                     /locus_tag="VGB85_31990"
     CDS             complement(3465..3794)
                     /locus_tag="VGB85_31990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEY0138765.1"
                     /translation="MHQVHPHYAAIYRVVRGVPRGRVATYGQIALLCGLPGRARQVGY
                     ALHALPPGSPVPWQRVLNARGDISLGHEAGERQRRLLLAEGVELDERGRVDLRRFQWK
                     PRHMRFL"
     gene            complement(3972..>4111)
                     /locus_tag="VGB85_31995"
     CDS             complement(3972..>4111)
                     /locus_tag="VGB85_31995"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEY0138766.1"
                     /translation="VVQETTMKDAGVGNCIAQAVKRWTFPKPEGGGSVIVTYPFVLEP
                     G"
BASE COUNT          544 a         1347 c         1595 g          625 t
ORIGIN      
        1 cgcgcagcag cagggtcgga caggcgatct gcttcaggaa tgccgtcacc tgcgcctcgg
       61 tcatgcgcag gcgcgagggc aggcgcaggc gagagtcggc gcgccagtcc cagcggcccg
      121 gcagggcctc gaacaggccg cggcgcagca gggtctgggc ggagctcggc tgcatctgcg
      181 tcgcctcggc gagccggcgg ccgacgacct cggggtcggg gtagccgggg cgctcgccgc
      241 tgcggcgggt cgcggcgagc tcggcggcga gggactcgcg caggcgctcg ggggcgctct
      301 cgtcggggtc ggtgagcggg ccgaggccct cgagcatcac gaggcgttgg acgcggtcgg
      361 gccaggtccc ggcgagcaac gcggccacgc cggcgccgag ggagtggccg agcagggaga
      421 acttttgcca gccgagggcg tcggcgacgg cggcgacgtc cgcgacgtag tcgatgaagt
      481 gataaggggc gccggcgcgg cgctgcgaga ggccatggcc ggcgaggtcg agggcgacga
      541 cgcggcagcc ggacagcagg ggtgcgaggc cgtcgaagct ggcggcgttg tcgagccagc
      601 cgtgcatggc gagcacgggc atgccatcgt cggggccatg gacgcgggcg gccagcgaca
      661 ggccggagat ctcgatcgtg cgctcgacga cgggcatggg cccggttttg gcccaagtcg
      721 cgggcggacg caatgcgacc ggcgcttcgc gcgtacgatg atgcgcgctc atggggtctg
      781 ggcagacggg tcgcgctccc gcatgctcgc ccggcgcgtg aggtgacctt gaacccggcg
      841 aacgaagcga tctggatcgg ctacacgacg ctgtaccgcg agggcggcgc gaagttcgag
      901 cgcgccgcgc tcacgctcgc cgccgagaag cgggccgctt tcccggcgtc ggtggtccgc
      961 tgcgagcggg tcgagtcgaa ggcggagttt cgcgcggcga tgcagcggat cgccgacgag
     1021 gggctcgtgc tgcgcgagct gcacctcgtc gtccacgccg gcatgtacgg gccgatgttc
     1081 ggcagcgtcg cgtggccgga gcagtttagc ccgcacgagt ggcgcatgat gaagctgccc
     1141 tttgggccag gtgccgaggc gttcttccac gcgtgccggt cggcgcggtg gttcgcgccg
     1201 ttcttcgccc gcaccttcgg ggtcccggcc cacggctatc actggtacac gacggtgtcg
     1261 tcggccccgg accggttccg ctgggacggg ctcgtcggac ccgaggcgcc gctgtacatc
     1321 ttcggcatgc cgggcaacaa gtcgcacggg gtgccgggca ccgtgcgaaa gtacctcggg
     1381 ctgatcgcgc ccgagcgtat gcgtcgcttc gagccggcgc cgatcgaagg cgacccgagc
     1441 tacgacgccg tcgcggccca gtacgaccgg gtgttttccg acatccgcgt gcgcagcgac
     1501 gagtggcggt ggatcgacgc ccggatcccc gccggcgcgc gcgtgctcga cgtcggctgc
     1561 ggcaacgggg ctttattaaa attgctggcg ccgtgtatca ccagcggggt cggggtcgac
     1621 gcctcgcgtg gcatgatcga ccgggcccgg gcccgcaccg cgggcgtgcc caacctgcgc
     1681 tttgaagtga tcgacgggcc gtccctgccg ctggaggaca acagcgtcga cgtcgtggtc
     1741 tcgctgctgt cgtttcgcta cctcgactgg gacccgatca tgcgagagtt cgcccgcgtg
     1801 ctcgcgcccg gcggccgcct gctggtggtc gacatggtcg ccgcgccgct ggggctgcgc
     1861 gatatcccgg ggctgctgcg cgacaaggcg cgcacgctgg tccagcagct gcgccaccgc
     1921 gagtatcacg gcgagctggc gcgcatggtc tcggacccga agtggcagga catgctccgc
     1981 tacaacccga tccgcgcgat ccacgagctg cgctggtatt ttaagagtcg gttcccggac
     2041 ggcaccgtcg agacgctcaa caccggctgg cacgcgcgcg tgctcgcgtt cgacaccggg
     2101 cccttcgagc agggcaacgt cgacccgcag tcgttcccgt gaactgtcat gaagctcggc
     2161 gtgctcgact gggggatcgg ggggatcgac ttttacgcgc ggtggaaggc gcggcacccg
     2221 gaggtgccgg tggtgtactg gtccgacagc ggagcgacgc cgtacgggaa gctggggcgt
     2281 gacgcgctgg cgcagcgcgt ggcgggcgtg gtgagggcgc tgcaggggcg cggggtgaca
     2341 cacgtggtcg tcgcctgcaa cgcggcgagc acggtgatcg ggcatccggc gctcgacctc
     2401 ggcgtcgagg tatgtggggt gatcgggccg gcgatcgcgg cgacgctcgc cgatccgggg
     2461 cggatcgtcg gcgtgatcgg cgggtggcga acgatctcgt cggggctgta tcgggatggg
     2521 ttgatcgcgg gcgggcggcg cgtgatctcg cgggtggccc agccgctgtc ggcgatggtc
     2581 gagcgcgggg agctcgactc gccggcggtg cacgcggagc tggggcggat cttgcggccg
     2641 ctgcggcgcg tcgaggcgct ggtgctcgcc tgcacgcact acccggcgct gctcgggccc
     2701 attcgacggg tgctaccggg agtcaggatc gtcgacccgg ccgcggcggc cctcgccgag
     2761 gtcgagcgcc gctggtgtct ctcgggacag ggcgaagcgg acatgttcct gacgacaggt
     2821 gacccgcagg cgacgcggga ggccgcgcgc ctggcgttcg gcgtcacgct gccgccggtg
     2881 cggcgcgtga cgctgtgatc acttcttgcc gaacttctcg atgcaggcgc cgagcaggtc
     2941 gggcacggcc ttgttgagct cctgcggggc gccggcgagg gcgttggcct gacaggccgc
     3001 ggtctcgatc gtgcccttga ggcacgactc cttcatgtcg gcgagctgct tggtcttctg
     3061 catcggccag ttggcgggga accacttgac catgaaggcg tccatcgcga cgttgagctc
     3121 ctcgcacgcc ggcgcgagct tcttgggcag cttggcgggc agcacggtca gcgcgtcgac
     3181 ctgcttgacc ttgttggcgg cggcctcctc ggcggccttg gccttggcct cgcgctcggc
     3241 cttgaacttg ttgggcgcct cggccttggc gggcgcctcg acggcggcct cctccttctt
     3301 gacctcggcc ggcttgctgt caccgccgca cgcggtcacc cacagcagcg ccgtcacacc
     3361 cagggttcgc gtcatgcatg agctcatcgc gcggcagctt gccacggcga gcgcgcaggc
     3421 agcaagccac gaatgaacgc ggacgggggc ctgcgctgaa gcgctcagag gaagcgcatg
     3481 tgccggggtt tccactggaa gcggcgcagg tcgacgcggc cgcgctcgtc gagctcgacg
     3541 ccttcggcca gcaggagccg gcgctggcgc tcgccggcct cgtgcccgag gctgatgtcg
     3601 ccgcgggcgt tgaggacccg ctgccacggc acggggctgc ccggcgggag cgcgtgcagg
     3661 gcgtagccga cctggcgcgc gcgcccgggg agcccgcaca gcagggcgat ctggccgtag
     3721 gtcgccacac ggccgcgggg gactccacgg acgacccggt agatcgcggc gtagtgcggg
     3781 tgcacctggt gcatcgaggc gaagctagca ggccgatctc gcgcggtcca gcgcgacgaa
     3841 aaaagcggga gcccggcttt catgccgggc tcccgcctgt cctcgcaaga tgtctctcgg
     3901 gtcatgtcgt gagggccagg ccctcacgtg gagaccaggc gatcagctgg atcagcctgt
     3961 atcaggctgt atcagcccgg ctcgagcacg aaggggtagg tcacgatgac gctgccgccg
     4021 ccctcgggct tggggaaggt ccagcgcttg acggcctgcg cgatgcagtt gccgacgccg
     4081 gcgtccttca tggtggtctc ctggacgacg g
//
LOCUS       DASWNJ010001918         1622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_173781, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001918 DASWNJ010000000
VERSION     DASWNJ010001918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173781"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1571)
                     /gene="carB"
                     /locus_tag="VGB85_32000"
     CDS             complement(<1..1571)
                     /gene="carB"
                     /locus_tag="VGB85_32000"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY0138767.1"
                     /translation="MPKRTDLKHVMVIGSGPILIGQAAEFDYSGTQACRVLRDEGIRV
                     ILVNSNPATIMTDPEFADATYIEPITPEFVAKVIEKERPDALLATLGGQTALNAAMAL
                     DADGTLEKYGVELIGASIEAIERGENRQSFKRIVEELGGESARSVIAHSLDDCLNAAG
                     DLGYPMVVRPSFTMGGSGSGMAYDEADLRMIAGSGLAASPTTEVLLEESIIGWKEYEL
                     EVMRDRADNVVVVCSIENLDPMGVHTGDSITVAPAMTLTDREFQHLRDLSIGIIRAVG
                     VDTGGRNIQFAVNPDDGRVVVIEMNPRVSRSSALASKATGFPIAKIAAKVAIGYTLDE
                     IPNDITVREDGQSTPAGFEPTLDYVVVKVPRFAFEKFPGADPTLTTHMKSVGEAMAIG
                     RNFTEALQKALRSLESPRASFDWSHQHVELDKAALLEAVRVAHDGRLRVVMDAIRAGA
                     TTDEVHEATGIDPWFVDQLCLINEVAVETIDAVELTPALLRRAKRHGFSDAQLGRMRD
                     MSEAVVRGVRHALGIR"
BASE COUNT          237 a          568 c          553 g          264 t
ORIGIN      
        1 cggatgccga gggcgtggcg cacgccgcgc acgacggcct cgctcatgtc ccgcatccgg
       61 cccagctggg cgtcggagaa cccgtgccgc ttggcgcggc gcaacagcgc gggcgtcagc
      121 tcgaccgcgt cgatggtctc caccgcgacc tcgttgatca ggcagagctg gtcgacgaac
      181 cacgggtcga tgccggtggc ctcgtgcacc tcgtccgtcg tcgcgccggc ccggatcgcg
      241 tccatcacga cccgaagccg cccgtcgtgc gcgacccgga ccgcctcgag cagcgcggcc
      301 ttgtcgagct cgacgtgctg gtgggaccag tcgaacgacg cccgcgggct ctccagcgag
      361 cggagcgcct tctgcagggc ctcggtgaag ttgcgcccga tcgccatcgc ctcacccacc
      421 gacttcatgt gcgtggtgag cgtcgggtcg gcgcccggga acttctcgaa ggcgaagcgc
      481 ggcaccttca ccacgacgta gtcgagggtc ggctcgaagc cggcgggcgt gctctggccg
      541 tcctctcgga ccgtgatgtc gttggggatc tcgtcgagcg tgtagccgat ggcgaccttc
      601 gccgcgatct tggcgatcgg gaacccggtg gccttcgacg ccagcgcgct cgaccgcgag
      661 acccggggat tcatctcgat gacgaccacc cgaccgtcgt cggggttgac ggcgaactgg
      721 atgttgcggc cgccggtgtc gacgccgacc gcgcggatga tgccgatgga gaggtcgcga
      781 aggtgctgga actcccggtc ggtcaaggtc atcgcgggcg ccaccgtgat cgagtcgccg
      841 gtgtgcacgc ccatcgggtc gaggttctcg atggagcaga cgacgaccac gttgtcggcg
      901 cggtcgcgca tcacctcgag ctcgtactcc ttccagccga tgatcgactc ctccaggagc
      961 acctcggtgg tcggcgacgc ggcgagcccc gagccggcga tcatccggag gtccgcctcg
     1021 tcgtaggcca tccccgaacc ggagccgccc atcgtgaagg agggccgcac caccatcggg
     1081 tagccgaggt caccggcggc gttcagacag tcgtcgagcg agtgggcgat gaccgaccgg
     1141 gccgactctc cgccgagctc ctcgacgatc cgcttgaacg actgccggtt ctcaccgcgc
     1201 tcgatcgcct cgatggacgc gccgatcagc tccacgccgt acttctccag cgtcccgtcc
     1261 gcgtcaagcg ccatcgcggc gttcagcgcg gtctggccac cgagcgtcgc cagcagcgcg
     1321 tcggggcgct ccttctcgat gaccttggcg acgaactctg gcgtgatcgg ctcgatgtac
     1381 gtcgcgtcgg cgaactccgg gtccgtcatg atcgtggcgg gattggagtt caccaggatc
     1441 acccggatgc cctcgtcgcg gagcacccgg cacgcctgcg tgccggagta gtcgaactcc
     1501 gccgcctgcc cgatgaggat cggcccggag ccgatgacca tgacgtgctt cagatcggtc
     1561 cgcttaggca ttgcggttct cctccatgag gtcgacgaag cgctggaaca gcggcgccgc
     1621 gt
//
LOCUS       DASWNJ010001919         4117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_629889, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001919 DASWNJ010000000
VERSION     DASWNJ010001919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629889"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3030)
                     /gene="rpoC"
                     /locus_tag="VGB85_32005"
     CDS             complement(<1..3030)
                     /gene="rpoC"
                     /locus_tag="VGB85_32005"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEY0138768.1"
                     /translation="MRDIFSFFEKPKEATYFNALRIGIASPKTIREWSFGEVKNPETI
                     NYRTFKPERDGLFCAKIFGPIKDYECLCGKYKRMKHRGVVCEKCGVEVIHSKVRRERA
                     GHIDLATPVAHIWFLKSLPSRIGNILDIPLTKLEKVLYCEAYIVIDPKDSGLEERELL
                     SEERFDQLVDELGPEAFVAGMGAEAIKELLSRIDPVVEARALRTEIAGATSEAKRKKA
                     AKRLRVVEAFRNSGNKPEWMMLDVIPVLPPDLRPLVPLDGGRFASSDLNDLYRRVINR
                     NNRLKRLQELAAPEIIIRNEKRMLQEAVDALFDNGRRGKTITGPNKRPLKSLSDMLRG
                     KSGRFRQNLLGKRVDYSGRSVIVVGPELRLHEVGIPKKMALELFKPFIYNRLEAQGLV
                     TTIKSAKRMVEKEADEVWDILDDVIKEHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQL
                     HPLVCTAFNADFDGDQMAVHLPLCIESQMEARVLMMSTNNILSPAHGRPIIVPSQDIV
                     LGLYYMTRERSFAHGSANEDPAGDGYFAGHFGSPKEVRMAYDHGALHLQAKIKVRMEP
                     GGEMINSTAGRVLLFEGGIPKRIPFSMVNKNLGKKELNELIDNCYRLCGQKATVLMAD
                     YLRSTGYTEATKAGISICMDDMVIPEAKKKLLASATAEVKRIMAQYADGLITSGERYN
                     KIIDIWANVSEQIANAMMEGISKTTLADERTGKTQIQPSFNSIYIMADSGARGSRQQM
                     RQLSGMRGLMAKPSGEIIEFPITTNFREGLTVLQYFISTHGARKGLADTALKTANSGY
                     LTRRLVDVAQEAVITEFDCGTIQGLDMTALVESGEIIERLGDRILGRVPLEPVYRPGS
                     DETIIEEGELFDEAKVAAVEEAGIQSVKIRSVLTCEAMRGICAKCYGRDLARGNQVDV
                     GEAVGIIAAQSIGEPGTQLTMRTFHIGGVGNIQTEQTTLEARFGGEVKLEDIELVHGR
                     EDKEGPYAVVMNRHAQLHLLDEKGRIRERYALVYGAKL"
     gene            complement(3067..>4117)
                     /locus_tag="VGB85_32010"
     CDS             complement(3067..>4117)
                     /locus_tag="VGB85_32010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEY0138769.1"
                     /translation="IVEALARRLEKDVDEIETIYREKIAKLTKGDELPPGVIKMVKVY
                     VAIKRKMQVGDKMAGRHGNKGVISRILPTEDLPYLEDGTPVDLVLNPLGVPSRMNVGQ
                     ILEVHLGWAAFELGQQIDRYMQTKFSSDVLRTQLKKIYETKEAQRFIDGLNDDDVSRF
                     ADKLRRGVHMATPVFDGALETEVKTTLQKAGLPEHGQAILFDGRTGEAFEENVTVGIM
                     YMLKLHHLVDDKIHARSIGPYSLVTQQPLGGKAQFGGQRLGEMEVWALEAYGAAYALQ
                     ELLTVKSDDVVGRMRMYESIVKGQPSMQAGVPESFNVLLKELQALCLNIELVDVNAET
                     GVVTNTVRDQMSAAE"
BASE COUNT          590 a         1247 c         1488 g          792 t
ORIGIN      
        1 cagcttggcg ccgtagacga gggcgtagcg ctcgcggatg cggcccttct cgtcgaggag
       61 gtggagctgc gcgtggcggt tcatgacgac cgcgtacggg ccctccttgt cctcgcggcc
      121 gtggacgagc tcgatgtcct cgagcttgac ctcgccgccg aagcgcgcct cgagggtggt
      181 ctgctcggtc tggatgttac cgacgccgcc gatgtggaag gtacgcatcg tcagctgggt
      241 gccgggctcg ccgatcgact gggcggcgat gatgccgacc gcctcgccga cgtcgacctg
      301 gttgccgcgg gcgaggtcac gcccgtagca cttggcgcag atgccacgca tcgcctcgca
      361 ggtgagcacg ctgcggatct tgaccgactg gatgccggcc tcctcgacgg cggccacctt
      421 ggcctcgtcg aacagctcgc cctcctcgat gatcgtctcg tcgctgcccg ggcggtagac
      481 cggctcgagc ggcacgcggc cgaggatgcg gtcgccgagg cgctcgatga tctcgccgga
      541 ctcgaccagc gcggtcatgt cgaggccctg gatcgtcccg cagtcgaact ccgtgatcac
      601 ggcctcctgg gcgacgtcga ccaggcggcg ggtcagatag ccggagttgg ccgtcttgag
      661 cgccgtgtcg gcgaggccct tgcgggcgcc gtgggtggag atgaagtact ggaggacggt
      721 cagaccctcg cggaagttgg tcgtgatcgg gaactcgatg atctcgccgg agggcttggc
      781 catgaggccg cgcataccgg agagctggcg catctgctga cgcgagccgc gggcgcccga
      841 gtcggccatg atgtagatcg agttgaacga cggctggatc tgggtcttgc cggtgcgctc
      901 gtcggcgagg gtcgtcttgg agataccctc catcatcgcg ttggcgatct gctcggagac
      961 gttcgcccag atgtcgatga tcttgttgta gcgctcgccg gaggtgatca agccgtcggc
     1021 gtactgggcc atgatgcgct tgacctcggc ggtggccgac gcgagcagct tcttcttggc
     1081 ctcggggatg accatgtcat ccatgcagat cgagatgccg gccttggtcg cctcggtgta
     1141 cccggtgctg cgcaagtagt cggccatcag gacggtcgcc ttctgcccgc acaggcggta
     1201 gcagttgtcg atcagctcgt tgagctcctt cttgccgagg ttcttgttga ccatcgagaa
     1261 cgggatccgc ttggggatgc caccctcgaa cagcaggacg cggcccgccg tgctgttgat
     1321 catctcgccg ccgggctcca tgcggacctt gatcttggcc tgcaggtgca gggcgccgtg
     1381 gtcgtaggcc atgcggacct ccttcggcga gccgaagtgg ccggcgaagt agccgtcgcc
     1441 ggcggggtcc tcgttggcgc tgccgtgcgc gaaggagcgc tcgcgggtca tgtagtagag
     1501 cccgagcacg atgtcctgcg acggcacgat gatcggccgg ccgtgcgcgg ggctgaggat
     1561 gttgttggtc gacatcatca gcacgcgcgc ctccatctgc gactcgatgc agagcgggag
     1621 gtggacggcc atctggtcgc cgtcgaagtc ggcgttgaag gcggtgcaga ccagcgggtg
     1681 gagctggatc gccttgccct cgatcaggac gggctcgaag gcctggatgc cgaggcggtg
     1741 gagggtcggc gcgcggttga ggagcacggg gtgctccttg atcacgtcgt cgaggatgtc
     1801 ccacacctcg tcggcctcct tctcgaccat gcgcttggcg ctcttgatgg tcgtgacgag
     1861 gccctgggcc tcgaggcggt tgtagatgaa cggcttgaac agctcgagcg ccatcttctt
     1921 ggggatgccg acctcgtgca ggcgcagctc ggggccgacg acgatgaccg agcggccgga
     1981 gtagtcgacg cgcttgccga gcaggttctg ccggaagcgg ccggacttgc cgcggagcat
     2041 gtcggacagc gacttgagcg ggcgcttgtt cgggccggtg atcgtcttgc cgcggcgacc
     2101 gttgtcgaac agggcgtcga cggcctcctg cagcatccgc ttctcgttgc ggatgatgat
     2161 ctccggggcg gcgagctcct ggaggcgctt gaggcggttg ttgcggttga tgacgcgacg
     2221 gtacaggtcg ttgaggtccg agctggcgaa gcggccgccg tccaggggga cgagcggacg
     2281 caggtccggc gggaggaccg ggatgacgtc gagcatcatc cactcgggct tgttgccgct
     2341 gttgcggaac gcctcgacga cgcgcaggcg cttggcggcc ttcttgcgct tggcctcgct
     2401 ggtggcgccg gcgatctcgg tacggagcgc gcgcgcctcg acgacggggt cgatgcggga
     2461 cagcagctcc ttgatcgcct cagcgcccat gccggcgacg aaggcctcgg ggccgagctc
     2521 gtcgacgagc tggtcgaagc gctcctcgct gaggagctca cgctcctcga ggcccgagtc
     2581 cttggggtcg atgacgatgt aggcctcgca gtagaggacc ttctcgagct tcgtcagcgg
     2641 gatgtcgagg atgttgccga tgcgcgacgg gaggctcttg aggaaccaga tgtgcgcgac
     2701 gggggtggcg aggtcgatgt ggccggcgcg ctcgcgacgg accttggagt ggatgacctc
     2761 gacgccgcac ttctcgcaga cgacgccgcg gtgcttcatg cgcttgtact tgccgcagag
     2821 gcactcgtag tccttgatcg gtccgaagat cttggcgcag aacaggccgt cacgctcggg
     2881 cttgaacgtg cggtagttga tggtctcggg gttcttgacc tccccgaagg accactcgcg
     2941 gatcgtcttg ggcgaggcga tgccgatccg gagcgcgttg aagtaggtgg cctctttggg
     3001 cttctcgaag aaactgaaga tgtctctcat ggccgtagtc ctgacttctg gtacttctgg
     3061 gatccgctac tccgccgccg acatctggtc gcgcacggtg ttggtgacga cgccggtctc
     3121 ggcgttgacg tcgaccagct cgatgttcag gcacagggcc tggagctcct tgaggagcac
     3181 gttaaaggac tcggggacgc ccgcctgcat gctgggctga cccttgacga tcgactcgta
     3241 catgcgcatg cggccgacga cgtcgtccga cttgacggtg agcagctcct gcaacgcgta
     3301 ggcggcaccg taggcttcga gggcccacac ctccatctcg ccgaggcgct ggccgccgaa
     3361 ctgggccttg ccgccgaggg gctgctgggt gaccagcgag tacggcccga tgctgcgcgc
     3421 gtggatcttg tcgtccacga ggtggtgcag cttgagcatg tacatgatgc cgacggtgac
     3481 gttctcctcg aacgcctcgc cggtgcggcc gtcgaacagg atcgcctggc cgtgctcggg
     3541 caggccggcc ttttgcagcg tcgtcttgac ctcggtctcg agggcgccgt cgaacaccgg
     3601 ggtggccatg tggacaccgc ggcgcagctt gtcggcgaag cgcgagacgt cgtcgtcgtt
     3661 gaggccgtcg atgaagcgct gggcctcctt ggtctcgtag atcttcttga gctgggtgcg
     3721 aagcacgtcg ctcgagaact tcgtctgcat gtagcggtcg atctgctggc cgagctcgaa
     3781 ggcggcccag ccgaggtgga cctcgaggat ctggccgacg ttcatgcgcg acgggacgcc
     3841 gagggggttc aagacgaggt cgacgggggt gccgtcttcc aggtacggga ggtcctcggt
     3901 cggcaggatg cgcgagatga cgcccttgtt gccgtggcgg ccggccatct tgtcgccgac
     3961 ctgcatcttg cgcttgatgg cgacgtagac cttgaccatc ttgatgacgc cgggcggcag
     4021 ctcgtcgccc ttggtcagct tggcgatctt ctcgcggtag atggtctcga tctcgtcgac
     4081 gtccttttcg aggcggcgcg cgagggcttc gacgatc
//
LOCUS       DASWNJ010001920         1777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_33808, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001920 DASWNJ010000000
VERSION     DASWNJ010001920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33808"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VGB85_32015"
     CDS             complement(<1..390)
                     /locus_tag="VGB85_32015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138770.1"
                     /translation="MSPRTRTIVARCALLAGLVLASCGLYEGRRKFIGQFFVDGAVPG
                     DTPVWPRAPEGDGLRPAARVRVVLLDGLSRAHALQLPGLSAICGAGQELQLDTGFPTV
                     SLPVQSALWTGMTQQQSGLQYHIGKLPA"
     gene            complement(437..>1777)
                     /locus_tag="VGB85_32020"
     CDS             complement(437..>1777)
                     /locus_tag="VGB85_32020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138771.1"
                     /translation="TWARQLRDEALVGYIDRMPGVVREPLDASAEAASDRPRLSYADA
                     AACTLEACEVLAGLHAGGFSGDRHDPISFAPEHLRVVAHGPEWHIAWQLPGYARPGPL
                     PPMTDADIDDIFADGDEDGSPIVRDLRGLYGLFTDLCDGAATPDDRVQVAALATLAQF
                     GQRVGGASPFVSVGLLAQLLAPLAPPRSTARARGIRFVNDLPPVRLDWDAVIAEGEAI
                     LADPSRPRYLMWRGRDLAVDGWIELPLAAAYHQRASRAFAQADLAPALRDSERAAALD
                     DHAPYHTTRAIVLDALGRTAESRAAIALATSGARSPGPSAQELARFLATRGLFALHDG
                     AGDHGLADLRRACELHPAATYFHGLAAALAAAGDSEAAVAAERRAVVVARGPDETHHR
                     WALVARLYALGRVDEARAQAETAVRQWPRQHGRFLRYFGEPPTWREEDDFPFHD"
BASE COUNT          242 a          650 c          690 g          195 t
ORIGIN      
        1 cgcgggcagc ttgccgatgt gatactggag gcccgactgc tgctgcgtca tgccggtcca
       61 cagcgcgctc tggaccggca gcgacacggt cgggaagccg gtgtcgagct gcagctcctg
      121 acccgcgccg cagatcgccg agagcccggg cagctgcagc gcgtgcgcgc gcgaaagacc
      181 gtcgagcagc accacgcgca cgcgggcggc cggacgcagc ccgtcgccct ccggcgcgcg
      241 cggccacacc ggcgtatcgc caggcaccgc gccgtcgacg aagaactggc cgatgaactt
      301 cctgcggccc tcgtagagcc cgcacgaggc gagcacgagg ccggccagca gcgcgcagcg
      361 ggcgacgatc gtgcgggtcc gcggagacat ggcgggcagg gtaggcgatc cgcgcgacgg
      421 cgtcagcacc cggagatcag tcgtggaacg ggaagtcgtc ttcctcgcgc cacgtcggcg
      481 gctcgccgaa gtagcgcagg aagcggccgt gctggcgcgg ccactggcgc accgcggtct
      541 cggcttgcgc gcgggcctcg tcgacccggc cgagcgcgta caggcgggcg acgagcgccc
      601 agcggtggtg cgtctcgtcg gggcccctgg cgacgacgac ggcgcggcgc tcggccgcga
      661 cggcggcctc gctgtcgccc gcggcggcga gcgccgcggc gaggccatga aagtatgtcg
      721 cggccgggtg gagctcgcag gcgcggcgca ggtcggcgag gccgtggtcg ccggcgccgt
      781 cgtgcagcgc gaacaggccg cgagtcgcca ggaagcgcgc cagctcctgc gcgctcgggc
      841 ccggcgagcg cgctccggac gtcgccaggg cgatcgcggc gcgcgactcg gcggtgcggc
      901 cgagcgcgtc gaggacgatc gcgcgggtcg tgtgatacgg cgcgtggtcg tcgagcgccg
      961 cggcccgctc gctgtcccgc agcgccggcg cgaggtcggc ctgcgcgaac gcccgcgagg
     1021 cccgctggtg atatgcggcg gcgagcggca gctcgatcca gccgtcgacc gcgagatcac
     1081 ggccccgcca catcaggtat cggggccgcg agggatcggc gaggatcgcc tcgccctcgg
     1141 cgatcacggc gtcccagtcg aggcgcacgg gcggcaggtc gttgacgaac cggatcccac
     1201 gcgcccgcgc ggtcgatcgc ggcggagcga gcggcgcgag cagctgcgcg agcaagccga
     1261 cgctgacgaa cgggctggcc ccgccgacgc gctggccgaa ctgcgccagg gtcgcgagcg
     1321 cggccacctg cacccgatcg tccggcgtcg cggcgccgtc acacaggtcc gtgaacagcc
     1381 cgtacagccc acgcaggtcg cggacgatcg ggctgccgtc ctcgtcaccg tcggcgaaga
     1441 tgtcatcgat gtcggcgtcg gtcatcggcg ggagcggacc cggccgggcg tacccgggga
     1501 gctgccatgc gatgtgccac tcgggtccgt gcgcgacgac ccgcaggtgc tccggcgcga
     1561 acgagatcgg gtcgtggcga tcgccggaga agccgcccgc gtgcaggccc gcgagcacct
     1621 cgcaggcctc gagcgtgcac gcggccgcgt ccgcgtacga cagccgcggg cgatcgctcg
     1681 ccgcctccgc ggacgcatcg agcggctcgc ggacgacgcc cggcatgcga tcgatgtagc
     1741 cgaccagcgc ctcgtcgcgc agctgcctcg cccacgt
//
LOCUS       DASWNJ010001921         2413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_527928, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001921 DASWNJ010000000
VERSION     DASWNJ010001921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527928"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /gene="dacB"
                     /locus_tag="VGB85_32025"
     CDS             <1..1020
                     /gene="dacB"
                     /locus_tag="VGB85_32025"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEY0138772.1"
                     /translation="TGGLVLDTTQFDRDELPPGFDQKDELVAYRAPGGALSVNFGTYV
                     VLARPGDKEGEAALAAIDPPVPSIKLENKATTEAGARTRLRVGLEPDAKGHGVTIQLA
                     GTIGLDAAPAEYRYPVASPAEYTGEVFRLVLGQRGIKLGKTAVRTSPIAADAERLAIV
                     RSQPLSVLVRSVNKLSNNYMAEHILKTLDPATPQSYAGGLARVHAWLRELEMPGDVRM
                     TNGSGLYDTNRISPVQLTHLLTRVHRDFRVAGDFLASLPIAGADGTLRSRLQDTGAER
                     FVRAKTGSLDQVSALSGYAGAVGKPPIAFSILVSGLDKWKAPLVKQAQDQIAAILAAE
                     AAARP"
     gene            1323..2372
                     /locus_tag="VGB85_32030"
     CDS             1323..2372
                     /locus_tag="VGB85_32030"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY0138773.1"
                     /translation="MQVGVITNPNSKKNYRRPQRRRSLEQAVGRHGVVRETASLDDLP
                     RVVGELLDAGCKYWVADGGDGTLHWVLNSVYAAVQARTPAGQALDLPIVVPTNGGTVD
                     FVARKAGLRGDADSIVRRLVARIERDAPISTIRVDTCRVLGKAHDAGAPRFDRIGLAA
                     ALGGASAAFFDRFYALPKDRGALAIASVIGAAAGSALVHSLAPPLRRFLPGELGDVDS
                     HNDFFRPTRARIEVDGRTLPYSTFVSLQVGAIDINLAGVVRCFRHAREGGVLHFQALS
                     TSPLGMVRNVPAMFLGTPIVGEGVYDDRAVNVVITAEDGERLGPVIDGEQFSGLDRME
                     LSLGPALRIPTLLSN"
BASE COUNT          338 a          944 c          788 g          343 t
ORIGIN      
        1 accggcggcc tcgtgctcga caccacgcag ttcgaccgcg acgagctccc gcccggcttc
       61 gatcaaaagg acgagctcgt cgcctatcgc gcccccggcg gggcgctgag cgtcaacttc
      121 ggcacctacg tcgtgctcgc gcggccgggc gacaaggagg gtgaggcggc cctcgccgcg
      181 atcgacccgc cggtgccctc gatcaagctc gagaacaagg cgaccaccga ggccggcgcc
      241 cgcacccgcc tgcgcgtcgg cctcgagccg gacgcgaagg gtcacggcgt cacgatccag
      301 ctcgccggca cgatcggcct cgacgccgcg ccggccgagt atcgctaccc cgtcgcctcg
      361 ccggccgagt acacgggcga ggtgttccgc ctcgtgctcg gccagcgcgg gatcaagctc
      421 ggcaagaccg ccgtgcgcac ctcgccgatc gccgccgacg ccgagcgcct cgccatcgtc
      481 cgctcgcagc cgctgtcggt gctggtccgc agcgtcaaca agctgtcgaa caactacatg
      541 gccgagcaca tcctcaagac cctcgacccc gccacgccgc agagctacgc gggcgggctg
      601 gcgcgcgtgc acgcgtggct gcgcgagctc gagatgccgg gcgacgtccg catgaccaac
      661 ggctcgggcc tctacgacac caaccgcatc tcaccggtcc agctgaccca cctgctgacg
      721 cgcgtgcatc gcgacttccg ggtcgccggt gacttcctcg cctcgctgcc gatcgccggc
      781 gccgacggga ccttgcgctc gcgcctgcag gacaccggcg ccgagcgctt cgtgcgggcc
      841 aagaccggca gcctcgatca ggtctccgcg ctgtcgggct acgcgggcgc ggtcggcaag
      901 ccgccgatcg ccttttcgat cctcgtcagc ggcctcgaca agtggaaggc gccgctggtc
      961 aagcaggccc aggaccagat cgcggcgatc ctcgcggccg aggccgcggc ccgcccataa
     1021 cgaccgctcg cgggatcgcg tcccccgcac gaacggcgtt aatcgcggcg ggccccacgg
     1081 acatcactca cggccccgcg gctgatcgcc gccgtgctgg cgcgcgaggt gtgtcgcgac
     1141 atgcacagcg aacatcgtga gggccgtaca tcgccgctgc gagcccgaca acggtgcgaa
     1201 acagcctgcg cacccgcgtc gtggccgtca catgcacgtt cctggccccc tggggttacc
     1261 ccggggctta tttgccggat cccgcggacc ggtcgtatgt ttgccgcgcc ctggagttcg
     1321 cgatgcaagt cggcgtcatc accaacccga actcgaagaa gaactatcgc cgtccgcagc
     1381 gtcgtcgcag cctcgagcag gctgtcggcc gccacggcgt cgtccgcgag accgccagcc
     1441 tcgacgatct tcccagggtc gtcggcgagc tgctcgacgc cgggtgcaag tactgggtgg
     1501 ccgacggcgg cgacgggacc ctgcactggg tgctcaacag cgtgtacgcg gccgtgcagg
     1561 cgcgcacccc cgccggtcag gccctcgacc tgccgatcgt cgtgccgacc aacggcggca
     1621 ccgtcgactt cgtcgcgcgc aaggccggcc tgcgcggcga cgccgactcg atcgtccgtc
     1681 gcctcgtcgc tcgcatcgag cgcgacgccc cgatctccac catccgcgtc gacacgtgcc
     1741 gcgtgcttgg taaagcccac gacgccggcg ccccgcgctt cgatcgcatc ggcctcgccg
     1801 ccgcgctcgg gggcgcctcc gcggccttct tcgaccgctt ctacgccctg ccgaaagatc
     1861 gcggcgccct cgctatcgcc tcggtgatcg gcgccgcggc cggcagcgcg ctggtccaca
     1921 gcctcgcccc gccgctgcgt cgcttcttgc ccggcgagct cggcgacgtc gacagccaca
     1981 acgacttctt ccgcccgacg cgcgcgcgca tcgaggtcga cggccggacc ctcccctact
     2041 cgaccttcgt ctcgctgcag gtcggcgcga tcgacatcaa cctcgccggc gtcgtgcgct
     2101 gcttccggca cgcgcgcgag gggggcgtcc tgcacttcca ggcgctgtcg acctcgccgc
     2161 tgggcatggt gcgcaacgtg ccggcgatgt tcctcggcac cccgatcgtc ggcgagggcg
     2221 tctacgacga ccgcgccgtc aacgtcgtga tcacggccga ggacggcgag cgcctcggcc
     2281 ccgtcatcga cggcgagcag ttctcgggcc tcgaccgcat ggagctgtcc ctcgggccag
     2341 ctctgcggat cccgacattg ctgagcaact gagcatctcc ggcgcgcccg cgtggcagcg
     2401 cgcgtggacg aac
//
LOCUS       DASWNJ010001922         1682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_138713, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001922 DASWNJ010000000
VERSION     DASWNJ010001922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138713"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VGB85_32035"
     CDS             complement(<1..470)
                     /locus_tag="VGB85_32035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138774.1"
                     /translation="MVLLSGFWTLAGCIFVPPPPLPEEIAAAEAAAAARKAAVRAGIP
                     ARPASAAGEGESEVATTTFTKGEEAPRGMNAAQLRAYNAAQGDPEASDFTLEEATAGL
                     PGQGDVLWAQLKTPRGVLECELFADKVPRTVANFVGLARGLRPVYDKASDSWVKR"
     gene            660..932
                     /gene="rpsT"
                     /locus_tag="VGB85_32040"
     CDS             660..932
                     /gene="rpsT"
                     /locus_tag="VGB85_32040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY0138775.1"
                     /translation="MANHKQAEKRNRQRLKRRTRNLMHLSTMRTYMKRVRKALDGKDN
                     EVTQTALPDAIQAISKAASKGVIHRNTASRYISRLTLAANKSKASS"
     gene            complement(1049..>1682)
                     /locus_tag="VGB85_32045"
     CDS             complement(1049..>1682)
                     /locus_tag="VGB85_32045"
                     /inference="COORDINATES: protein motif:HMM:NF044544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138776.1"
                     /translation="GNYAYKFEALKKDDDAVAFVLGEARAQRRQISREAAVTMVAAVG
                     TGRSELLGALEQAMLHAGEGPIGIENVEAMVARTREGNIFHLTDAVGRGDATAALTVL
                     AQMFSSGEKDTGVAMQVLAMLTRQIRLIFTAKVGGPDAIKLPPFLLRGLESQARGFTE
                     ARLRTAYASLVRLDRDLKGGSHVAYATPMMALQRWILDTCRALPGVDART"
BASE COUNT          258 a          634 c          553 g          237 t
ORIGIN      
        1 cgcttcaccc agctgtcgct ggccttgtcg tagaccggcc gcagaccccg ggccaggccg
       61 acgaagttgg cgaccgtgcg cggcaccttg tcggcgaaca gctcgcactc gagcacacca
      121 cgcggcgtct tgagctgggc ccacagcaca tcaccctgtc cgggcaggcc cgcggtcgcc
      181 tcctcgagcg tgaagtcgct ggcctccggg tcgccctggg ccgcgttgta ggcccgcagc
      241 tgcgcggcgt tcatgccccg tggcgcctct tcccccttgg tgaaggtcgt cgtcgccacc
      301 tcgctctcgc cctcgccggc cgcgctcgcc ggccgcgccg ggatcccggc gcgcacggcg
      361 gccttgcggg ccgcagcagc cgcctcggcg gccgcgatct cctccggaag cggcggcggc
      421 ggcacaaaga tgcagcccgc cagcgtccaa aaaccactga gcaggaccac gccccggccc
      481 atccgcggcc ccgtgggacg ggcgtggacg gggcaggaag acggcggcgc gggcgtgcga
      541 aacatcgtgg agtccaaagg cgggtgccgg agggtgtacc gtcgcgcgcg gcttgacaag
      601 gggcgggccg cggggccata aggccctcct cctaccgaaa cttcgagaag cgagcagacg
      661 tggcgaacca caagcaggca gagaagcgca atcgtcaacg gctcaagcgc cgcacccgga
      721 acttgatgca cctgtcgacc atgcgtacgt acatgaagcg cgtgcgcaag gccctggacg
      781 gcaaggacaa cgaggtcacc cagaccgccc ttcccgatgc catccaggcg atcagcaagg
      841 ccgcctccaa gggcgtcatc caccgcaaca cggcctcgcg gtacatctcc cgcctgaccc
      901 tggcggccaa caagtccaag gcgtcctcct gatcgcctga cgcctcgatc ggcccaccga
      961 tcccgatctc cggcctcgcc atgttcgcat ggcgccgtcg gatgatcgta tttaatcgat
     1021 cgttggctca tcgcgggaga gcccggactc aggtcctcgc gtcgaccccg ggcagcgccc
     1081 gacacgtgtc gaggatccac cgctgcagcg ccatcatcgg cgtcgcgtag gcgacgtgcg
     1141 agccgccctt gaggtcgcgg tcgagccgca cgagcgacgc gtaggcggtg cgcaggcggg
     1201 cctcggtgaa gccacgcgcc tgcgactcga gcccgcgcag caagaacggc ggcagcttga
     1261 tcgcgtcggg gcccccgacc tttgccgtga agatcagccg gatctgccgc gtgagcatcg
     1321 ccagcacctg catcgccacg cccgtgtcct tttcgccgga gctgaacatc tgcgcgagca
     1381 cggtcagcgc cgcggtcgcg tcgccgcggc cgaccgcgtc cgtcagatgg aagatgttgc
     1441 cctcgcgcgt gcgggcgacc atcgcctcga cgttctcgat cccgatcggc ccctcgcccg
     1501 cgtgcagcat cgcctgctcg agcgcgccga gcagctcgct gcggccggtg ccgaccgccg
     1561 cgaccatcgt caccgccgcc tcgcgggaga tctggcgccg ctgcgcccgc gcctcgccga
     1621 gcacgaaggc gaccgcgtcg tcgtccttct tgagcgcctc gaacttgtac gcgtagttgc
     1681 cc
//
LOCUS       DASWNJ010001923         5460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_669805, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001923 DASWNJ010000000
VERSION     DASWNJ010001923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669805"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VGB85_32050"
     CDS             complement(<1..201)
                     /locus_tag="VGB85_32050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEY0138777.1"
                     /translation="MTVDQTNPGPIDPRSSHGSAGPGCPVFLTGMMGAGKTTIAPLLA
                     AAWGVTWVDLDVRVAHIFGAGVA"
     gene            complement(198..749)
                     /locus_tag="VGB85_32055"
     CDS             complement(198..749)
                     /locus_tag="VGB85_32055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138778.1"
                     /translation="MSAGSILHGSPLQTLIVWVLGTCVAAVVWYAVFYRAAHDEWVAA
                     SNELGAARSDLEQARAARVRADGRARELDVAAAELSTERARVLPGAPEDLVFTVPALA
                     SAIGLEVVRWRPSELEVDGALLRAPVQVDARGRWPAALGLMTRVLTLPEVVALDRLAM
                     RTAPDGALELQFTLSTVQLREAP"
     gene            complement(754..1326)
                     /locus_tag="VGB85_32060"
     CDS             complement(754..1326)
                     /locus_tag="VGB85_32060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138779.1"
                     /translation="MIRINLLPAAARRRRRSRPGRERGVLALMSLGWAGLLTAGYLWI
                     AATEAEIAELRGQTTAVVRDRDAVRKTFDERALVDREQALQRERDALAQLATARRTPA
                     PLLTELAQIFAAGVDGPFVAGGPLTLRELRELGEGRWLLVASVADLPALTDLLRRLEA
                     SPRFAEVGDAEYARGDDGRLALRVTLTARE"
     gene            complement(1323..2351)
                     /gene="pilM"
                     /locus_tag="VGB85_32065"
     CDS             complement(1323..2351)
                     /gene="pilM"
                     /locus_tag="VGB85_32065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEY0138780.1"
                     /translation="MARHCIGLDIGSTAIKLVQLRTGRAGLALQNFGVAPVPTGAIAG
                     GAVIDAAGVAAALRALVLRIGLRGKDVALAVSGNGVIIKRIAVPQLEPAAFAQQIEWE
                     ARQLVSPPSRDDVLIDHTRLRQNPMTGEVEVLLVAAKKDLVQQYLRVVGEAGLRPVVV
                     DATAFALQNCVEAGQGFTSGETFAVVNVGAAASTISILAEGMPAYGRDISSGGNLYTE
                     ALRGLGLSAEDAERRKRDAEDPEIQQRMLPVSEAIAAEIQRSLDFFRGAVAQEPLSRI
                     VLSGGSALVPSLRQALAQRSRLPVTLLDPFARVQVDRRVDLGVLRSSAPAAAVAFGLA
                     LREEDDAR"
     gene            complement(2378..2815)
                     /locus_tag="VGB85_32070"
     CDS             complement(2378..2815)
                     /locus_tag="VGB85_32070"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0138781.1"
                     /translation="MTATSKQEPPIPADDPVAEVVADEAEESEAQQATRRGKKVVTAS
                     VATVPAAATVPAAVPAAASEPTAKAAKAKPAVQPNNVRKLRQAAMMSKAELARRAGVS
                     PLTIDRVESGCPCRMDTKRKILEALGLKPEAREQVFGPDIDQG"
     gene            3709..5184
                     /gene="glpK"
                     /locus_tag="VGB85_32075"
     CDS             3709..5184
                     /gene="glpK"
                     /locus_tag="VGB85_32075"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEY0138782.1"
                     /translation="MSADLILAIDQGTTGTTCLLVDAGLKVRARATREFPQHFPQPGW
                     VEHDPEELWRSVLAVIADVMQHADPKQIAAIGITNQRETSLVWDRRTGAPIHRALVWQ
                     DRRTAGVCEALKADGREPAIRERTGLVLDPYFSATKLAWVLDHVPGARTRARHGELAA
                     GTIDTYLVWRLTAGASHVTEPSNASRTLLWPLTGGGWDHDLCDLLRVPPQVLPEVRPC
                     TANFGVTKGVPGLPDGVPIHGIAGDQQSALFGQACFAVGEAKCTYGTGAFVMLNTGSR
                     PVPSQHGLLTTVAWQIGEETTYCLEGSAFMAGAVVQWLRDGLGIITKASEIEALARSV
                     EDSGGVTLVPAHAGLGAPHWRPYARGLITGLTRGTTRAHLARAALEGIALQISDLLLA
                     MAADTGAPLRTLKVDGGAAANDLLMQLQADFLGVPLARPTMLEATALGAVFMAGLGVG
                     LWKNTEALAAAWQEDRGFTPGPATPQRNELRRAWDRAISIA"
     gene            complement(5186..>5460)
                     /locus_tag="VGB85_32080"
     CDS             complement(5186..>5460)
                     /locus_tag="VGB85_32080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138783.1"
                     /translation="PQHVTAVDLDGAGFTPVCALELSDGEVVTGPAHACLVEDSGSGV
                     VWLVYGGAAGLWIAAGAARGERPRADEPGATFDAYLLVDRDALVFA"
BASE COUNT          732 a         2094 c         1906 g          728 t
ORIGIN      
        1 cgcgacgccg gcgccgaaga tgtgagcgac gcggacgtcg aggtcgaccc aggtcacgcc
       61 ccacgccgcg gcgagcagcg gcgcgatcgt cgtcttgccg gcgcccatca tgccggtgag
      121 gaacaccgga cagccgggcc ccgcgctgcc gtggctgcta cggggatcga tcggccccgg
      181 gttggtctga tcgacggtca tggcgcctcc cgcagctgca ccgtgctcag cgtgaattgc
      241 agctcgagcg cgccgtccgg ggccgtgcgc atggcaaggc gatcgagcgc gacgacctcg
      301 ggcagcgtca gcacccgtgt catcaagccg agggccgccg gccagcggcc gcgcgcgtcg
      361 acctgcaccg gcgcccgcag cagcgcgccg tcgacctcga gctccgatgg ccgccagcgc
      421 acgacctcga ggccgatcgc gctcgccagc gccggcacgg tgaacaccag gtcctccggt
      481 gcgccgggca gcacgcgggc gcgctctgtg gatagctccg ccgcggccac gtcgagctcg
      541 cgcgcgcggc cgtccgcgcg cacccgggcg gccctggcct gctcgaggtc gctgcgcgcc
      601 gcgccgagct cgttgctcgc ggcgacccac tcgtcgtgcg ccgcccgata gaacaccgcg
      661 taccagacga ccgccgcgac gcaggtgccg agcacccaga cgatcagggt ctgcagcgga
      721 gagccgtgga ggatggagcc tgcggacatc gtgtcattcc cgggcggtca gcgtcacgcg
      781 cagcgcgagc cggccgtcgt cgccgcgagc gtactctgca tcgccgacct cggcgaagcg
      841 cgggctcgcc tcgagccggc gcagcaggtc ggtcagcgcc ggcaggtcgg cgaccgaggc
      901 gaccagcagc cagcgcccct cgccgagctc gcgcagctcg cggagggtca gcggtccgcc
      961 ggcgacgaag ggtccatcga ccccggcggc gaagatctgc gccagctcgg tgagcagcgg
     1021 cgcgggtgtg cggcgggcgg tcgccagctg ggcgagggcg tcgcgctcgc gctgcagcgc
     1081 ctgttctcgg tcgacgagcg cgcgctcgtc gaaggtcttg cgcacggcgt cgcggtcgcg
     1141 gaccaccgcg gtcgtctgtc cgcgcagctc ggcgatctcc gcctcggtcg cggcgatcca
     1201 caggtagccc gcggtcagca gcccggccca gccgaggctc atcagcgcga gcaccccgcg
     1261 ctcgcggccg ggccgactgc gccggcgccg gcgggccgcg gccggcagga ggttgatgcg
     1321 gatcaccggg cgtcgtcctc ctcgcgcagc gccagcccga aggcgaccgc cgccgccggc
     1381 gcgctggatc tcaggacgcc gagatcgacg cggcggtcga cctgcacgcg cgcgaagggg
     1441 tcgagcaggg tgaccggcag gcgcgagcgc tgggccagcg cctgacgcag cgacggcacc
     1501 agcgccgagc cgccgctgag cacgatgcgc gacagcggct cctgggcgac ggcgccgcgg
     1561 aagaagtcga gcgaccgctg gatctccgcg gcgatcgcct ccgagaccgg gagcattcgt
     1621 tgctggatct ccgggtcctc ggcgtcgcgt ttgcggcgct cggcgtcctc ggccgagagc
     1681 ccgagcccgc gcagcgcctc ggtgtagagg ttgccgccgg agctgatgtc acgcccgtag
     1741 gccggcatgc cctcggcgag gatgctgatc gtcgaggccg cggcgccgac gttgacgacc
     1801 gcgaaggtct ccccgctggt gaagccctgg ccggcctcga cgcagttttg cagcgcgaag
     1861 gcggtcgcgt cgacgacgac cgggcgcagc cccgcctcgc cgacgacgcg caggtactgc
     1921 tgcacgaggt ccttcttggc ggcgacgagc agcacctcga cctcgccggt catggggttt
     1981 tgccggagcc gggtatggtc gatgagcacg tcgtcgcggc tgggcggcga gaccagctgc
     2041 cgcgcctccc actcgatctg ctgcgcgaac gcggccggct cgagctgcgg caccgcgatc
     2101 cgcttgatga tgacgccgtt gccggacacg gcgagggcga catccttgcc gcgcaggccg
     2161 atccgcagga ccagcgcgcg cagggccgcg gcgacgcccg ccgcgtcgat caccgcgccg
     2221 ccggcgatcg ccccggtcgg caccggcgcg acgccgaagt tctgcagcgc cagcccggcc
     2281 cgcccggtcc gcagctgcac gagcttgatg gccgtcgagc cgatgtcgag cccgatgcag
     2341 tgtctcgcca tggcctcgcc cccgtacgga tggcgcgtca gccctggtcg atgtccgggc
     2401 cgaacacctg ctcgcgcgcc tcgggcttga gccccagcgc ctcgaggatc tttcgcttgg
     2461 tgtccatgcg gcacgggcaa cccgactcga cgcggtcgat cgtcagcgga ctgacacccg
     2521 cacgccgcgc gagttcggcc ttgctcatca tcgccgcctg ccgcaacttg cggacgttgt
     2581 tgggctgcac cgccggcttc gccttcgcgg ccttcgccgt cggctcgctc gcagccgcgg
     2641 gcacagccgc aggcacggtg gcagccgcag gcacggtggc gacgctcgcc gtcacgacct
     2701 tcttgccgcg ccgcgtggcc tgctgcgcct cgctctcctc ggcctcatcc gccacgacct
     2761 cggcgaccgg gtcgtcggcg gggatcggcg gctcctgttt ggaggttgca gtcatcggca
     2821 cagccactcg caatgcactt cctacacatt ctcggccccg gcaagggctt cgtcaacgat
     2881 gggcccccgc ctcagtgata cgtaagtgaa tgttagtgat agggatttga taggtcattg
     2941 tagtgaatta tgcaggtgcg cagttcccac agggcgcccc tggcaccacg tgtaaattct
     3001 cgtaattgtt gaccaaagcg aattcccgtg ccgcgagggc gtcctgcggc ttcggcgcga
     3061 cccctggcgc gaccgatcgc cacccctacc tggccttgaa ttcacgtcgg agcgatccct
     3121 cgcgggcgtg atcccgcgcg gcttcgggcg gtcctccctg gagagatctc gctcgcccgg
     3181 aacgggcccc gaagtccccg ccccaggccg gagatccgcc tcggacctgc tcctggaccc
     3241 cgctggcgcc tgccctcgcg aggccgtcgc gctcggcgaa agccgacgcg ggccggcgcg
     3301 ccgagagcca cttcgaagcg cgtcagcgcg gtcgcgaggt ggtcacgctg tcgcgagatc
     3361 ggcgctcgtc gctgccgggg ctcggccaat ggatgcacgg cgacgagcgc ccgcgatcga
     3421 ccggcaccgc cgtcatcggc agccgctcga gcgcgcagcg cagcagccac gcgcgctcgc
     3481 gggccggctc gaagtggccc gcgtcgagct cggcccggat cgccgcgact cgtcgactcg
     3541 tcgagcgagc gtcgccggcg agtgcagcag ccgacgcctg gtccgcggcg tcgcccgatc
     3601 accagcagct tgcccgcggt cgacatcacc tcgccgaaca tcgtgtcgac gacaccggac
     3661 gtgtcgtggc gcatcggccg aaggcgctcg tgataatctc gcgcccgcat gtccgccgac
     3721 ctgatcctcg ccatcgacca agggaccacc gggaccacct gcctgctcgt cgacgccggg
     3781 ctcaaggtgc gcgcgcgggc gacccgcgag ttcccgcagc acttcccgca gcctggctgg
     3841 gtcgaacacg acccggagga gctgtggcgc tcggtgctcg ccgtcatcgc cgatgtcatg
     3901 cagcacgcgg acccgaagca gatcgcagcg atcggcatca cgaatcagcg cgagaccagc
     3961 ctggtctggg accggcgcac cggtgcaccg atccaccggg cgctcgtctg gcaggaccgg
     4021 cgcaccgcgg gcgtgtgcga ggcgctcaag gccgacggcc gcgagccggc gatccgcgag
     4081 cgcaccggcc tcgtgctcga cccctacttc tcggcgacca agctcgcgtg ggtcctcgac
     4141 cacgtcccgg gggcgcgcac ccgcgcccgg cacggcgagc tcgccgccgg cacgatcgac
     4201 acctacctcg tgtggcggct gaccgccggc gcgtcgcacg tcacggagcc gagcaacgcc
     4261 tcgcgcacgc tgctgtggcc gctgacgggt ggcggctggg atcacgacct ctgtgatctg
     4321 ctgcgcgtgc cgccgcaggt gctgccggag gtccgcccgt gcaccgccaa cttcggcgtc
     4381 acgaagggcg tacccggcct gcctgacggc gtcccgatcc acggcatcgc cggtgaccag
     4441 cagtccgcgc tgttcggcca ggcctgcttc gccgtcggcg aggccaagtg cacctacggc
     4501 accggcgcgt tcgtcatgct caacaccggc tcgcgcccgg tgccctcgca gcacggcctg
     4561 ctgaccaccg tcgcctggca gatcggcgag gagaccacgt attgcctcga gggcagcgcg
     4621 ttcatggccg gcgccgtggt ccagtggctg cgcgacggcc tcggcatcat cacaaaggcc
     4681 agcgagatcg aagcgctcgc gcgcagcgtc gaggacagcg gcggtgtcac cctcgtgccc
     4741 gcccacgccg gcctcggcgc cccgcactgg cggccctacg cgcgcggcct gatcaccggc
     4801 ctgacccgcg gcaccacccg cgcccacctc gcccgcgccg cgctcgaggg catcgccctc
     4861 cagatctccg acctgctgct cgctatggcc gccgacaccg gcgcgccgct tcgcacgctc
     4921 aaggtcgacg gcggcgccgc ggccaacgac ctcctcatgc agctccaggc cgacttcctc
     4981 ggcgtcccgc tcgcgcgccc gacgatgctc gaggcgaccg ccctcggcgc cgtgttcatg
     5041 gccggcctcg gcgtcggcct gtggaagaac accgaggcgc tcgccgccgc ctggcaggag
     5101 gaccgcggct tcacgccggg cccggcgacg ccgcagcgca acgagctccg ccgcgcgtgg
     5161 gaccgcgcga tctccatcgc ctgagctacg cgaagaccag cgcgtcgcgg tcgaccagca
     5221 agtacgcgtc gaaggtcgcg cccggctcgt ccgcacgcgg gcgctcgccc ctcgccgcgc
     5281 cagcggcgat ccacagcccc gccgcgccgc cgtacaccag ccacacgact ccgctgccgc
     5341 tgtcctcgac caggcacgcg tgcgccggtc cggtcaccac ctcgccgtcg ctcagctcga
     5401 gcgcgcagac cggcgtgaag ccggcgccgt cgaggtcgac cgcggtcacg tgctgcggct
//
LOCUS       DASWNJ010001924         2076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_33877, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001924 DASWNJ010000000
VERSION     DASWNJ010001924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33877"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /gene="nusA"
                     /locus_tag="VGB85_32085"
     CDS             <1..1248
                     /gene="nusA"
                     /locus_tag="VGB85_32085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY0138784.1"
                     /translation="LFQVLYVVEEVQSKHREISLEQAHRAGLEAEVGDELLFQIFYLD
                     ADKKRAEQQSKDYAEIEALQFGQHGFGRIAAQTAKQVIIQRVREAERENMFDAYKDKK
                     GELMTGIVRRFERGSIVVEVDNGKAEALLPAREQVPRESLRPGDRIVAYVKDVDKSAR
                     GPQIILSRTHRGLVEKLFEHEVPEIYEGIVRIEACAREPGARSKIAVSSRDRDVDPVG
                     ACVGIRGSRVQAVVQELRGEKIDIVPFNDDPARFVCNAIQPATVSRVLIDAERHTMEL
                     VVPDDKLSLAIGKKGQNVRLASRLTGWRIDIYSESKLRQMERRSLAEMAAIPGVGDTV
                     ASSLFQMGWRNLRDLAIADADELAQVQGIGSVDRAESIIEVANDAASGKLVLQVKYPE
                     PASDDQRMVVDPNEDEGYAGGEP"
     gene            1695..>2076
                     /locus_tag="VGB85_32090"
     CDS             1695..>2076
                     /locus_tag="VGB85_32090"
                     /inference="COORDINATES: protein motif:HMM:NF016635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEY0138785.1"
                     /translation="MAKIRVYELAKELGKDNKEMETVIRGLGVEIKGVMSTLDDDQAQ
                     MVRRRMQGGDRREPGRPSPSAGSPGGAASGAGQGNQAGQGGQGGQAPQAGQPASPAMV
                     IRRRSVGPRPGEDDETSAPPAMPSR"
BASE COUNT          357 a          723 c          708 g          288 t
ORIGIN      
        1 ctcttccagg tcctctacgt cgtcgaagag gtgcagtcca agcaccgcga gatcagcctc
       61 gagcaggcgc accgcgccgg cctcgaggcc gaggtcggcg acgagctgct gttccagatc
      121 ttctacctcg acgccgacaa gaagcgcgcc gagcagcagt ccaaggacta cgccgagatc
      181 gaggcgctgc agttcggcca gcacggcttc ggccgcatcg ccgcccagac cgccaagcag
      241 gtgatcattc aacgcgtccg cgaggcggag cgcgagaaca tgttcgacgc ctacaaggac
      301 aagaagggcg agctcatgac gggcatcgtc cgccgcttcg agcgcgggtc catcgtggtc
      361 gaggtcgaca acggcaaggc ggaggcgctg ctgcctgccc gcgagcaggt cccccgcgag
      421 tcgctgcgcc ccggcgaccg catcgtcgcg tacgtcaagg acgtcgacaa gtcggcccgc
      481 ggcccgcaga tcatcctgtc tcgcacgcat cgcggcctcg tcgagaagct gttcgagcac
      541 gaggtccccg agatctacga gggcatcgtt cgcatcgagg cctgtgcccg tgagccgggc
      601 gcccgcagca agatcgccgt gtcctcgcgc gaccgcgacg tcgacccggt cggcgcctgc
      661 gtcggcatcc gcggtagccg cgtgcaggcc gtcgtccagg agctgcgcgg cgagaagatc
      721 gacatcgtcc ccttcaacga cgaccccgcg cgcttcgtgt gcaacgcgat ccagccggcg
      781 accgtctcgc gcgtcctgat cgatgccgag cgccacacga tggagctcgt cgtcccggac
      841 gacaagctga gcctggcgat cggcaaaaag ggccaaaacg tgcgcctcgc gtcgcgcctg
      901 accggctggc gcatcgacat ctactccgaa agcaagctgc gccagatgga gcgccgctcg
      961 ctcgccgaga tggccgcgat cccgggcgtc ggcgacaccg tcgccagctc gctgtttcag
     1021 atgggctggc gcaacctccg cgatctggcg atcgccgacg cggacgagct cgcccaggtc
     1081 caggggatcg gctcggtcga ccgcgccgag agcatcatcg aggtcgccaa cgacgccgcc
     1141 agcggcaagc tggtcctcca ggtcaagtac ccggagccgg cgtcggatga ccagcgcatg
     1201 gtcgtcgatc ccaacgagga cgagggctac gccggaggtg agccctgagt atgcacgtgc
     1261 cgatgcgcac ctgtgtcggt tgccggcacg tccgcccgca ggcggatctc ctgcgcaccc
     1321 gttgcctgca cggaaaaccc gtgccggctc tggcgcgaac tcactcgcga gctgtccctt
     1381 cggacagcgc acgggtgggt cgcgcggcgt atttgtgtcc gtcgcggtcc tgcctgatcc
     1441 aggcggcccg ccgcggcggc ttcgcccgga cgttccaggc gggccccgtc accgccgacg
     1501 tggacgcgtt gtgggcggac ctgagtgaag cagtgaaccg agaatccgac ctgttgcagc
     1561 gaagcggcct ggcccgggcg gacatgccca agtcagatct agttactcgg acgccgtccc
     1621 cggcgccgat ccgtgtccac caactcctga cgctggcggc tgcaatgcgg gccccgggga
     1681 gaggtgcctg agttatggcg aagatccgag tttacgagct ggccaaagag ctgggcaaag
     1741 acaacaaaga gatggagacc gtcatccgcg gcctcggcgt cgagatcaaa ggtgtcatga
     1801 gcaccctcga cgacgaccag gcccagatgg tccgccgtcg gatgcagggc ggcgaccggc
     1861 gtgagccggg tcgtccgtca ccctcggccg ggtctcccgg cggagccgcg tccggagcag
     1921 gacagggtaa ccaagcaggt cagggcgggc agggcggtca agcaccgcaa gccggtcaac
     1981 ccgcgagccc cgcgatggtc attcgtcggc gcagcgtcgg gccccggccc ggcgaggacg
     2041 acgagaccag cgcgcctccg gcgatgccga gccgcg
//
LOCUS       DASWNJ010001925         8005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_704055, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001925 DASWNJ010000000
VERSION     DASWNJ010001925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 8005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8005
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704055"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..1316
                     /locus_tag="VGB85_32095"
     CDS             96..1316
                     /locus_tag="VGB85_32095"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0138786.1"
                     /translation="MEPLDITKIRKDFPVLKRMVNGKPLVYLDNAATSQKPMAMIKRM
                     NDLYSHEYSRPEEGHTLSREATRAFEATRSKVARLVNGNSSREVVFTRGATEGLNLIA
                     MAYGHELLKEGDEILVTEAEHHSNIVPWLLACKQNGARVKAAPLTPGGDIDVEKLEGM
                     LHDRVKLVSVTHVSNVTGGVQPVREITKIVHRHKIPVVVDGAQAVAHMPVDVRDIGCE
                     FYTGSGHKMGGPSSVGFLWGRADVLQSMPLADGGSNMSKNVTFEDVEANPIPERYEAG
                     EPAFAEVEAWSAAIDYWNDLGLDRIEAYELDLTEYATNKLREIPGVRVLGEPKRRLSV
                     LSFVVEGMSPSAVEKALDSEGIAVRAGKLAAQPMLRFFGVEEAVRASFLFYNTHEEAD
                     FFARVLTQIANSKS"
     gene            complement(1759..2097)
                     /locus_tag="VGB85_32100"
     CDS             complement(1759..2097)
                     /locus_tag="VGB85_32100"
                     /inference="COORDINATES: protein motif:HMM:NF021188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HEY0138787.1"
                     /translation="MPGRAVVLCLGTNPEVDMWTIKLSHDNPLPVEEAKRRLGAVASE
                     YARFYGVATRWEGDKMVLSGTLLDGHIDFSAASVDVDVTLGPGAQTQKDRSDVESGIQ
                     IAFAQYLRGK"
     gene            2210..2782
                     /locus_tag="VGB85_32105"
     CDS             2210..2782
                     /locus_tag="VGB85_32105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138788.1"
                     /translation="MLRFLAAAVTLAAIAASIYAAWSPIQLAEATLRADLGPHEYIGW
                     PGTALRLLYTVLVTASVLVLVLWPLNRTLARMKQNTRHRLALLGQCLALALMLWILLL
                     PALVWRALGVVNATWDSAPPRAETYRFITVVKHHRGREHVDYARADDPTSELSLFTIR
                     ADPNTEPGTPVTLYRHDGALGMAYVSTRPR"
     gene            2838..3476
                     /locus_tag="VGB85_32110"
     CDS             2838..3476
                     /locus_tag="VGB85_32110"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY0138789.1"
                     /translation="MSDELVFYTNPMSRGRIVRWMLEELGQPYRTEVLDYGTTMKAPE
                     YLAINPMGKVPAVRHGDAVVTEVAAICAYLADAFPEARLAPPANDRLRATYYRWLFFA
                     AGPTEAAVTNKSLGVVPTAEQRGMVGYGSLDEVMNALERAVSDREYIVGDSFTAADVY
                     VGSHIGWGMRFGSFERRPAFESYTSRLMARPAAVRAQEIDDALLAAAKKSAS"
     gene            3545..4033
                     /locus_tag="VGB85_32115"
     CDS             3545..4033
                     /locus_tag="VGB85_32115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138790.1"
                     /translation="MHTSHRGLAFLLSLGSLPLACTKNDNDTDDGGTSGTGSTTTPTT
                     TGDTGASDTGVTTGDPDLCVVYAKHIAECNPTESSAYDESLMYCQLYKKYGFEADGKP
                     CEDAFDALYVCLSNTDCAELANQDFGACEAEGAAISTACPTFGEADTDTEAPSETGSS
                     TT"
     gene            complement(4101..6557)
                     /locus_tag="VGB85_32120"
     CDS             complement(4101..6557)
                     /locus_tag="VGB85_32120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0138791.1"
                     /translation="MSAPNLPQRARVVVIGGGVIGCSVAYHLAHMGWRDVVLLERDRL
                     TSGTTWHAAGLMVTFGSTSETSTEMRKYTRDLYSRLEAETGVATGFNPVGFIEVACEA
                     DRLEEYRRVSAFNRYCGIDVQEISAAQVQDLFPLARTDDVLAGFYVKEDGKANPVDVT
                     MALAKGARLQGATILEGVPVTGVRQRRGVVTGVDTPYGSIEAEFVVNCTGMWARQLGA
                     KSGVNIPLQAAEHYYLITDKIAGISPDWPVLEDPGSFGYFREEIGGMMVGLFEPVCAP
                     WRVGGIPEDFSFGDLAPDWDRMGPYVETAMRRIPILMETGVKKFFCGPESFTPDLRPV
                     VGEAPELKNYFVAAGLNSIGILTGGGLGRVIAHWIVHGRPDVDVTGMNIDRLHTYQSN
                     PEYRRTRTVESLGMVYQCHYPTRSMQTARGAKLSPVHDRLAARGAFFKDVSGWEGADW
                     YAPAGVEPVVEKLTWGRHNWFPYWQAEHQATREGVILMDMSFMAKFMVQGRDAGRVLD
                     YISANSVDGEAGKITYTQWLNVGGTLEADLTVTKLDDENFWVVASDTAQRHVLTWMRR
                     NFPADAHAFVTDVTSGYAQINIQGPRSRALMQSLTTCDLSNEAFPFRTAREIDIGFAR
                     ALCVRITYLGELGYELYIPAEQAIHVYDRIVAAGEAFGLRHAGLKALASLRMEKGYRD
                     YGHDIDNTDSVLEAGLGFAVDFKKAAFIGRDAVLLKKAAGPLTRRIVQILVLDPEPML
                     FHAEVVRRDGRPIGYIRAGSYGHTVGGAVGLAMIEAGVPVDQAYLDAGTWEVDIGGRL
                     YPARASLRPLYDPEMRRVRS"
     gene            complement(6663..>8005)
                     /locus_tag="VGB85_32125"
     CDS             complement(6663..>8005)
                     /locus_tag="VGB85_32125"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEY0138792.1"
                     /translation="PAPMRTLAARLGRVVASAAHGFHRLEIDAAPRMSQLPRKASVVF
                     VLDRSRSNSEEELAAQLRIVDAYRSHVPDATIEIVAFDREARRVFGEFTGADKFAAAL
                     EQARMTGKLSPGNGSALERGLALAAEVLAGCSGPTRIVALTDARMRTRFTNRMADAGL
                     AGAPATTVTHVVIPEQDGYAFIRRDDAHALAPIADGHRGVLFIAAAPADDKTVPAQML
                     GLVRPIAIDHFKVTGMDLSDAGELPEVLREGDGYRAMIATPDPTRRVVISGKVWATPV
                     RRVVTHNEHFDIATAAFVFSEDEHHNLSPEEQRVVAFRGRAVSPVTSYLATEPGVRPS
                     VDGLEEEIGQGLGLGMIGMGGGGGGYGHIPAKPPTLEDLLARDIERCLKAHAPTGAYQ
                     LTLAVETTLVEIADVEARTSTHAPLRDCMTEATWALALPAADWPERMAHTITLP"
BASE COUNT         1293 a         2967 c         2557 g         1188 t
ORIGIN      
        1 gtcccctaca ccaccgtctg acccgcagat ccgccggatc gcggaccctc cccccgaaca
       61 ggccgcgccg catcggcgcg ttcaaggagc cacgtatgga acctctcgac atcacgaaga
      121 tccgcaagga cttcccggtc ctcaagcgca tggtcaacgg caagccgctg gtctacctcg
      181 acaacgcggc gaccagccaa aagccgatgg cgatgatcaa gcggatgaac gacctctact
      241 cgcacgagta ttcgcgcccg gaggagggcc acacgctcag ccgcgaggcg acgcgtgcgt
      301 tcgaggcgac gcgctccaag gtcgcgcgcc tggtcaacgg caactcgtcg cgtgaggtcg
      361 tgttcacgcg cggcgcgacc gaaggcctca acctgatcgc catggcatac ggccacgagc
      421 tgctcaagga gggcgacgag atcctcgtca ccgaggccga gcaccactcc aacatcgtcc
      481 cctggctgct cgcctgcaaa caaaacggcg cgcgggtgaa ggccgcgccg ctgacccccg
      541 gcggcgacat cgacgtcgag aagctcgagg gcatgctgca cgaccgcgtc aagctcgtgt
      601 cggtcacgca cgtatccaac gtcaccggcg gcgtacagcc cgtccgcgag atcacgaaga
      661 tcgtgcatcg ccacaagatc cccgtggtcg tcgacggcgc gcaggccgtg gcgcacatgc
      721 cggtcgacgt gcgtgacatc ggctgcgagt tctataccgg ttcgggacac aagatgggcg
      781 gcccctcgag cgtcggtttc ctgtggggcc gggccgatgt gttgcagagc atgccgctgg
      841 ccgacggcgg ctcgaacatg tcgaagaacg tcaccttcga ggacgtcgag gccaacccga
      901 tccccgagcg ctacgaggcc ggcgagccgg ccttcgccga ggtcgaggcg tggagcgccg
      961 cgatcgacta ctggaacgac ctcggcctcg accgcatcga ggcctacgag ctcgacctca
     1021 ccgagtacgc gaccaacaag ctgcgcgaga tccccggcgt gcgcgtgctg ggcgagccga
     1081 agcggcgcct gtcggtgctg tccttcgtcg tcgagggcat gtcccccagc gccgtcgaga
     1141 aggccctcga cagcgagggc atcgccgtgc gcgccggcaa gctcgccgcc cagccgatgc
     1201 tgcgcttctt cggcgtcgag gaggccgtgc gcgcctcgtt cctgttctac aacacccacg
     1261 aggaggccga cttcttcgcc agggttctca cccagatcgc aaattcgaag tcctgaggcc
     1321 tcggctggga actcccccat gcgatcgacg ggcgatggct gcgcagaaac ccgtccagga
     1381 gggcctgtag ggcattttgc gcaagattgg ccagaactcg ccggcccgtg cttgcgagag
     1441 tacgacacgg gcccatagac acctcgtccc ctcaacaagg agcatgcccg tgaaccgcga
     1501 aaacatgcag tcgtctgaat cgtcgcttta cgccactcct cttgcgcagt ccagcgtgac
     1561 cgagtccttc tccgcgcgac cgagcgaggc agacatcgcc cgcgccgccc agcgccgctc
     1621 gacccgcacg ccgcacggca cccactgttc gggcgcccgc tagcaagccc gcagcgagcc
     1681 ccccgctcgc cgagccgccg agatgccagg ccatcgctcg atggcacgat acgtgaatag
     1741 taaaaaatcg gcccgcggtc acttgccgcg gagatactgc gcaaacgcga tctggatccc
     1801 gctctcgacg tcgcttcggt ccttttgggt ctgggcgccg ggcccgagcg tcacatcgac
     1861 atcgaccgaa gcggcgctga aatcgatgtg accgtcgagc agggtgccgc tgagcaccat
     1921 tttgtcgccc tcccagcggg tcgccacccc gtagaagcgt gcatattcgc tggcgaccgc
     1981 cccgaggcga cgcttggcct cctcgaccgg cagggggttg tcatggctga gcttgatcgt
     2041 ccacatatcg acctccgggt tggtcccgag gcatagcacc acggcgcgac ccggcatcgc
     2101 gcgaatgcac gcgccgtccg accggcgttc cccgaggcac ggcggatgcc cggcgggccc
     2161 gcctgcacca catcgcggcg caccgcgcgg ctgatagact gccgcgcgca tgctccggtt
     2221 cctggcagcg gccgtcaccc tcgccgcgat cgctgcctcg atctacgcgg cctggagccc
     2281 gatccagctc gccgaggcca cgctgcgcgc cgacctcggc ccccacgaat acatcggctg
     2341 gccgggcacc gccctgcgcc tgctctacac cgtgctcgtg accgcgagcg tgctcgtgct
     2401 cgtgttgtgg ccgctcaacc gcacgctcgc gcgcatgaag cagaacaccc gccaccgcct
     2461 cgcgctgctc gggcagtgcc tcgcgctggc cctgatgctg tggatccttc tgctccccgc
     2521 cctcgtgtgg cgcgccctcg gcgtcgtcaa tgccacatgg gacagcgccc ccccgcgcgc
     2581 cgagacctac cgcttcatca ccgtcgtcaa gcaccaccgc ggccgcgaac acgtcgacta
     2641 cgcccgcgcc gacgacccca cgagcgagct ctcgctgttc acgatccgcg ccgacccgaa
     2701 caccgagccc ggcacgcccg tcacgctgta ccgccacgac ggcgcgctcg gcatggccta
     2761 cgtctcgacg cggccccgat gacccgcgtg ctccgcactc tgtccacggc cgcgggcagc
     2821 cggtagagtc ccgcaccatg tccgacgagc tcgtcttcta caccaacccg atgtcacgcg
     2881 gccggatcgt tcgatggatg ctcgaggagc tcggccagcc ctaccgcacc gaggtcctcg
     2941 actacggcac gaccatgaag gcgcccgagt acctggcgat caacccgatg ggcaaggttc
     3001 cggcggtccg ccacggcgac gccgtcgtca ccgaggtcgc cgcgatctgc gcctacctgg
     3061 ccgacgcgtt ccccgaggcc cgcctggccc cacccgcgaa cgaccgcctg cgcgcgacct
     3121 actaccggtg gttgttcttc gccgcaggtc cgaccgaggc ggccgtgacc aacaagtcgc
     3181 tcggcgtcgt gccgaccgcg gagcagcgtg ggatggtcgg ctatggctcg ctcgatgagg
     3241 tgatgaacgc gctcgagcgc gccgtctcgg accgcgagta catcgtcggt gacagcttca
     3301 ccgcggccga tgtctacgtc ggctcgcaca tcggctgggg catgcgcttc ggatcgttcg
     3361 agcgccgccc ggcgttcgag agctacacca gccggctgat ggcccgcccg gcggccgtgc
     3421 gcgcgcagga gatcgacgac gccctgctcg ccgccgcgaa aaagtccgcg agctgaggcg
     3481 cgcgccggct cgcgttttcg ggcgttgccg ctgctgcgtc ctcgcccgta cgatgggccc
     3541 cttcatgcat acctcccacc gcggcctcgc cttcttgctc tcgctcggca gcctcccgct
     3601 cgcctgcacc aagaacgaca acgacaccga cgacggaggc acctcgggca ccggcagcac
     3661 caccacgccg acgacgaccg gcgacaccgg tgcatcggac accggcgtca caaccgggga
     3721 cccggacctc tgcgtcgtct acgccaagca catcgccgag tgcaacccga ccgagagcag
     3781 cgcctacgac gagtccctca tgtactgcca gctctacaag aagtacggct tcgaggccga
     3841 cggaaagccc tgcgaggacg ccttcgacgc cctctacgtg tgcctgtcca acaccgactg
     3901 cgccgagctc gctaaccagg acttcggcgc ctgtgaggcc gagggcgccg ccatctcgac
     3961 cgcctgcccg accttcggcg aggccgacac cgacaccgag gccccaagcg agaccggcag
     4021 ctcgacgacg tgatcgcctc acgccacgcc gccgtggcga gctcgcctca tgacgacctt
     4081 cgtgtccggc gcgacctctc tcagcttcgc acgcgccgca tctccgggtc gtacagcggc
     4141 cgcaacgacg cgcgcgccgg gtacaggcgg ccgccgatgt cgacctccca ggtgcccgcg
     4201 tcgaggtacg cctgatcgac cggcacgccc gcctcgatca tcgccaggcc gaccgcgccg
     4261 cccaccgtgt gcccgtacga cccggcccgg atgtagccga tcggccggcc gtcacgacgg
     4321 accacctccg cgtggaacag catcggctcc gggtcgagca cgaggatctg cacgatcctc
     4381 cgcgtcagcg gtcccgcggc ctttttgagg agcaccgcgt cccgcccgat gaacgcagct
     4441 ttcttaaaat cgacagcgaa gccgaggccc gcctcgagca ccgaatccgt gttgtcgata
     4501 tcgtggccgt agtcgcgata tcccttctcc atgcggaggc tggcgagcgc cttgaggccc
     4561 gcgtggcgca gcccgaacgc ctcccctgcg gcgacgatgc ggtcgtacac gtggatcgcc
     4621 tgctcggccg ggatatacag ctcgtagccg agctcaccga ggtacgtgat ccgcacgcac
     4681 agcgcccttg cgaagccgat gtcgatctct cgcgccgtgc ggaacgggaa cgcctcgtta
     4741 ctgagatcgc aggtcgtcag cgactgcatc agcgcccgcg accgcggccc ctggatgttg
     4801 atctgcgcgt acccggaggt cacatcggtg acaaacgcat gcgcgtccgc ggggaagttc
     4861 cggcgcatcc acgtcagcac gtgccgctgc gccgtatccg acgcgaccac ccaaaagttc
     4921 tcgtcgtcga gcttggtgac cgtcaggtcg gcctcgagcg taccgccgac gttgagccac
     4981 tgcgtatatg tgatcttccc ggcctcgccg tcgaccgagt tcgccgagat ataatcgagc
     5041 acgcgcccgg cgtcgcgccc ctggaccatg aacttcgcca tgaagctcat gtccatcagg
     5101 atcacgccct cacgcgtcgc ctggtgctcc gcctgccagt acgggaacca gttgtggcgc
     5161 ccccacgtca gcttctcgac caccggctcg acgcccgcag gcgcgtacca gtccgcccct
     5221 tcccacccgc tgacatcttt aaaaaatgcc ccgcgcgcgg ccaggcggtc gtgcaccggc
     5281 gagagcttgg ccccgcgcgc cgtctgcatc gatcgcgtcg ggtagtggca ctgatacacc
     5341 atgccgagcg actcgaccgt acgcgtgcgg cgatactccg ggttgctctg ataggtgtgc
     5401 aggcggtcga tgttcatccc ggtgacatcg acatcgggcc gaccgtgcac gatccagtgg
     5461 gcgatcacgc ggccgaggcc gccgccggtc aagatcccga tcgagttgag gccggcggcg
     5521 acgaagtagt tcttgagctc gggcgcctcg ccgaccacgg gccgcaggtc gggcgtaaaa
     5581 ctctccggac cgcagaagaa ttttttgacc cccgtctcca tcaagatcgg gatccggcgc
     5641 atcgccgtct ccacatacgg gcccatgcgg tcccagtcgg gcgcgaggtc gccgaacgag
     5701 aagtcctccg ggatcccacc gacgcgccac ggcgcgcaca ccggctcgaa cagcccgacc
     5761 atcatcccgc cgatctcctc acgaaaatag ccgaacgagc ccgggtcctc gagcaccggc
     5821 caatcgggcg agatgccggc gatcttgtcg gtgatcagat aatagtgctc ggccgcctgc
     5881 agcggaatgt tgacacccga cttcgcgccg agctggcgcg cccacatgcc ggtgcagttg
     5941 acgacgaact cggcctcgat gctgccgtac ggcgtatcga cgccggtgac gacgccacgt
     6001 cgctggcgga cgccggtcac cggcacgccc tcgaggatcg tcgcgccctg caggcgggcg
     6061 cccttggcca gcgccatcgt cacgtcgacc gggttggcct tgccgtcctc cttcacataa
     6121 aagccggcga gaacatcgtc ggtgcgcgcc agcgggaaca ggtcctgcac ctgcgccgcc
     6181 gagatctcct gcacgtcgat gccgcagtag cggttgaacg ccgacacgcg gcgatactct
     6241 tccagacggt cggcctcaca cgcgacctcg atgaagccga ccgggttgaa gccggtcgcc
     6301 acgcccgtct ccgcctcgag ccggctgtag aggtcgcgcg tgtacttgcg catctccgtc
     6361 gaggtctcgg aggtcgagcc gaaggtgacc atcaggccgg ccgcgtgcca cgtcgtgccc
     6421 gaggtcaggc ggtcgcgctc gagcaacacg acgtcgcgcc agcccatgtg ggccaggtgg
     6481 taggcgaccg agcagccgat gacgccgccg ccgatgacga ccacgcgcgc ccgctggggc
     6541 aagttgggcg ctgacatgag gcggcagcat agccgacgcg ggccggcggc gcatgtcgca
     6601 gaacgatgct cgaacaacga tcgcggcgac tcgtgtaggg atggtcggat ccgtgccgaa
     6661 cctcagggca gcgtgatcgt gtgggccatg cgctcgggcc agtcggcggc gggcagcgcg
     6721 agcgcccacg tcgcctccgt catgcagtcg cgcagcggcg cgtgcgtgga ggtccgcgcc
     6781 tcgacatcgg cgatctcgac gagcgtcgtc tcgaccgcga gcgtcagctg atacgcccct
     6841 gtcggcgcgt gggccttgag acagcgctcg atgtcccgcg cgagcaggtc ctcgagcgtc
     6901 ggcggcttcg cggggatgtg gccataaccg ccgcccccgc cgcccatgcc gatcatgccg
     6961 aggccgaggc cttgaccgat ctcctcctcg aggccgtcga ccgacgggcg cacgccgggc
     7021 tcggtcgcca gatacgaggt caccggcgac accgcgcggc cgcgaaacgc gaccacgcgc
     7081 tgctcctcgg gcgacaaatt gtggtgctcg tcctccgaga acacgaaggc cgcggtggcg
     7141 atgtcgaagt gctcgttgtg cgtgacgacc cggcgcaccg gcgtcgccca caccttgccg
     7201 ctgatgacga cgcggcgcgt cgggtccggc gtggcgatca tcgcccgata gccgtcgccc
     7261 tcgcgcagca cctccggcag ctcgccggcg tccgacaggt ccatgccggt gaccttgaag
     7321 tgatcgatcg cgatcgggcg caccagcccg agcatctgcg ccggcaccgt cttgtcgtcg
     7381 gccggcgccg ccgcgatgaa cagcaccccg cggtgaccgt cggcgatcgg cgcgagcgcg
     7441 tgcgcgtcgt cgcggcggat gaacgcataa ccatcctgct cggggatcac cacgtgcgtc
     7501 accgtcgtcg ccggcgcgcc cgcgaggccc gcgtcggcca tccggttggt gaagcgcgta
     7561 cgcatgcggg cgtccgtcag cgccacgatg cgcgtcggcc cgctgcagcc cgccagcacc
     7621 tcggccgcga gcgccaggcc gcgctcgagc gcgctgccgt tgcccgggga cagcttgccc
     7681 gtcatcctgg cctgctcgag cgccgccgcg aatttgtccg ccccggtgaa ttcgccgaac
     7741 acgcgccgcg cctcgcggtc gaacgcgacg atctcgatcg tcgcgtcggg cacgtgcgag
     7801 cggtaggcgt cgacgatgcg cagctgcgcg gccagctcct cctcgctgtt cgagcgcgag
     7861 cggtcgagca cgaacaccac cgaggccttg cgcggcagct ggctcatccg cggcgccgcg
     7921 tcgatctcga gccggtgaaa gccgtgcgcc gccgaggcga ccacgcggcc gaggcgggcc
     7981 gcgagcgtgc gcatcggcgc cggcg
//
LOCUS       DASWNJ010001926         5069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_669861, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001926 DASWNJ010000000
VERSION     DASWNJ010001926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669861"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VGB85_32130"
     CDS             <1..692
                     /locus_tag="VGB85_32130"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0138793.1"
                     /translation="VETQDPTRPELRSALFHRDEIEIHDTWDTCGLRGTGSHDYSVKD
                     VFVPAARTFSLLGAPVHDAPLYRQPFFGTLAAGVASVSLGIARAAIDALVTLATSKQP
                     PGARKTLAHRELVQLQVARAEGELQAARAGLFAAVTAAADEAETTGVASLRTRALVRV
                     AACHTAQACARAVDLAYEAGGGSSIYSRSPLQRCFRDAHVVTAHVMVNSTSLTTAGRV
                     LLGVEGDSTMV"
     gene            complement(696..1310)
                     /locus_tag="VGB85_32135"
     CDS             complement(696..1310)
                     /locus_tag="VGB85_32135"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEY0138794.1"
                     /translation="MPSEADLMEVAADLAGDIGAAELTRRMADNIRALRKARGYSLDD
                     MARRSGVSRASLSQVETAKTNPTIAILWKIAAGLEVPFSALLGEDNAERVSVLRRPDQ
                     RVLRSADGHFESRPLTPAGVLTGVEVYELRLAPHTVHASDAHAAGTSESLTVLVGTLR
                     LRVGDEVHDLSAGDAACFVADLAHSYENPGRTPTLVHNVIVYAR"
     gene            1489..2118
                     /locus_tag="VGB85_32140"
     CDS             1489..2118
                     /locus_tag="VGB85_32140"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY0138795.1"
                     /translation="MTELVLLRHGETVGQSSIRLYGSTDIPLSPIGEAQLGRAAAALS
                     PHQFVRVLTSPLQRARRSAEIVLAAQREPAALEVVPDFREIDFGAWEGWTYAEAEARD
                     PDGYRRWHSEGTAFTYPAGESRSAFHARVHAAAHALLGAPGPGPILGVLHKGVIKAVL
                     AALTGQSPDAVASLPIALASLHRLRRTGSTWELVATGETAHLGDHDLGG"
     gene            2155..2985
                     /locus_tag="VGB85_32145"
     CDS             2155..2985
                     /locus_tag="VGB85_32145"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0138796.1"
                     /translation="MRRLAALVVALAVAPNVHAAPAYTPTVGQSGKDVIWVPTPDALV
                     DRMLAMAQVRPEDEVVDLGSGDGRLVILAAKKYGARARGVEYNAELVALSRQAAKAAG
                     VGKRVKLEQADIFKIDFTAATVVTLYLLPNINKRLRPILLRMRPGTRIVSHQFTMEDW
                     SPDETSYVEQRAAHLWIVPAQVAGAWSLAMPDGTTLDLDLEQKFQQISGRVALASVRA
                     GLRDAKLRGDQIRFSFVDMQGVTHEFAGRVLPGRMEGTVTTSGASKPWTATPRPAAQP
                     "
     gene            complement(2992..3408)
                     /gene="msrB"
                     /locus_tag="VGB85_32150"
     CDS             complement(2992..3408)
                     /gene="msrB"
                     /locus_tag="VGB85_32150"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY0138797.1"
                     /translation="MSERTVQKSDEEWRGVLTPDQFRVLRQAGTERPFTGEYWNHTAA
                     GQYTCAGCGAVLFESTTKFDAGCGWPSFYEALERDAIEERVDTTHGMRRVEVLCKRCG
                     GHLGHVFPDGPKPTGLRYCINSLSLAFKPTDGSGAR"
     gene            3620..4834
                     /locus_tag="VGB85_32155"
     CDS             3620..4834
                     /locus_tag="VGB85_32155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138798.1"
                     /translation="MLRPILLLACPILLLSACTVQVDADPEVDAGTTDAEESEGHSTQ
                     GPDDATTGPAPTTEPEATSSTSTTTPADPTGEPIDDPANDGCVPQDDVIAVHEAVLDS
                     STGEPDPSSSSSGGTAGDTEGDTDAEGDTDFESDTDGAEECAPLPSHTPSKLDTNCKC
                     DIQKEHRACKTPDAQAGTRYCDGGEWGPCIVEPACDPGSFIVCYYCDEQFGTMLSDCL
                     LHDGVPVDNSGEDCATPLVLSFDGRAVEYTSAHAEFAMADGDGCGARDWPTAATPWLA
                     IDLDRNGNIDGGHELFGTGSRLAGRVDNGFAALAALDSNADGQISGADARFAEIVVWS
                     DHDADRRSTMWELQPLASHGVVAIDLGYAFDASRCDARGNCEGERASFVFETADGARS
                     SGEIVDVRLSCQ"
BASE COUNT          725 a         1903 c         1735 g          706 t
ORIGIN      
        1 tggtcgagac ccaggacccg acccgacccg agctgcgcag cgccctgttc caccgcgacg
       61 agatcgagat ccacgacacc tgggacacct gcgggctgcg cggcaccggc agccacgatt
      121 actcggtcaa ggacgtgttc gtgcccgccg cgcgcacctt ctcgctgctc ggcgcgccgg
      181 tccacgacgc gccactttac cgccagccgt ttttcggcac gctcgcggcc ggcgtcgcgt
      241 cggtctccct cggcatcgcc cgcgcggcga tcgacgcgct ggtgaccctc gccaccagca
      301 agcagccccc cggcgcgcgc aagaccctcg cgcaccgcga gctcgtgcag ctgcaagtcg
      361 cccgcgccga gggcgagctg caggccgcgc gcgccggcct gttcgccgcg gtcacggccg
      421 ccgccgacga ggccgagacc accggcgtcg cctcgctgcg cacccgcgcg ctcgtgcgcg
      481 tcgccgcctg ccacaccgcc caggcctgcg cccgcgcggt cgacctcgcc tacgaggccg
      541 gcggcggcag ctcgatctac agccggagcc ccttgcagcg ctgctttcgc gacgcccacg
      601 tggtcaccgc ccacgtcatg gtcaactcga ccagcctgac gaccgcgggc cgcgtgctgc
      661 tcggcgtcga gggcgacagc accatggtct gagcctcagc gcgcgtacac gatcacgttg
      721 tgcacgaggg tcggcgtgcg ccccgggttc tcgtagctgt gcgcgaggtc ggcgacgaag
      781 caagccgcgt cgcccgcgga caggtcatgc acctcgtcgc cgacgcgcag gcgcagggtg
      841 ccgacgagca cggtcaggct ctcgctggtg ccggccgcgt gggcgtcgga cgcgtgcacg
      901 gtgtgcgggg cgaggcgcag ctcgtagacc tcgacgccgg tgagcacgcc ggcgggggtc
      961 aggggtcggc tctcgaagtg gccgtcggcg gaccggagca cgcgctggtc ggggcggcgc
     1021 agcacgctga cgcgctccgc gttgtcctcg ccgagcaggg ccgagaacgg cacctcgagg
     1081 cccgcggcaa ttttccagag gatcgcgatc gtcgggttgg tcttcgcggt ctcgacctgc
     1141 gagaggctcg cccgcgacac gccggatcgg cgggccatgt cgtcgagcga gtagcccctc
     1201 gccttgcgca gcgcgcggat gttgtcggcc atgcggcgcg tcagctcggc cgcgccgatg
     1261 tcccccgcga ggtcggccgc gacctccatc agatcggcct cggaaggcac gtccgcggag
     1321 ccccccgcgg ccgctccggc agcagcccca gcaggagatc cagcgtccgt ggcggaggcc
     1381 gtggacctgg ccgggcggtt gcccttcggc gttcgagctg ccataagcgc tcctctcttg
     1441 tatgccaaac ttccacagga ataaaccccg gaagccaggc ggtcgtcgat gaccgagctc
     1501 gtgctcctgc gtcacggcga gacggtgggc cagtcgtcca ttcgcctcta cgggtccacg
     1561 gacatccccc tgagcccgat cggggaggcc cagctcgggc gcgccgctgc ggccttgagt
     1621 ccccaccagt tcgtccgggt cctgacctcg ccgttgcagc gcgcgcgtcg ctccgccgag
     1681 atcgtgctcg cggcccagcg cgagcccgcc gccctcgagg tcgtgcccga cttccgcgag
     1741 atcgatttcg gcgcctggga gggctggacc tacgccgagg ccgaggcccg cgaccccgac
     1801 ggctaccgcc gctggcacag cgagggcacc gccttcacct accccgccgg cgagtcccgc
     1861 tcggccttcc acgcgcgcgt ccacgcggcc gcgcacgcgc tgctcggcgc gcccggcccc
     1921 ggcccgatcc tcggcgtgct gcacaagggc gtcatcaagg ccgtgctcgc cgcgctgacg
     1981 ggccagagcc ccgatgccgt cgccagcctg ccgatcgcgc tcgccagcct gcaccgcctg
     2041 cgccggaccg gctcgacatg ggagctggtc gccaccggcg agacggccca cctcggcgac
     2101 cacgacctcg gcggctgagc ccgaactgcc ggcgctcgcg ctatgctcgg gcgcatgcgc
     2161 aggctcgcag ccctcgtggt cgccctggcg gtcgcgccga atgttcacgc cgcgccggcc
     2221 tacacgccga cggtcgggca gtccggcaag gatgtcatct gggtcccgac gcccgacgcg
     2281 ctggtcgacc gcatgctggc gatggcgcag gtgcggcccg aggacgaggt cgtcgacctc
     2341 ggcagcggcg acggccggct cgtcatcctc gcggcgaaga agtacggcgc gcgcgcccgc
     2401 ggcgtcgagt acaacgccga gctggtcgcg ctgtcgcggc aggccgccaa agccgccggc
     2461 gtcggcaagc gcgtgaagct cgagcaggcc gacatcttca agatcgactt caccgcggcg
     2521 acggtggtca cgctgtacct gctgccgaat attaataaac gcctgcgacc gatcctgctg
     2581 cgcatgcggc cgggcacgcg catcgtctcg catcagttca cgatggagga ctggtcgccc
     2641 gacgagacct cctacgtcga gcagcgcgcc gcgcacctgt ggatcgtgcc ggcccaggtc
     2701 gccggcgcgt ggtcgctcgc catgcccgac ggcacgaccc tcgacctcga cctcgagcag
     2761 aaatttcagc agatcagcgg tcgcgtcgcc ctggcgtccg tgcgcgccgg cctgcgcgac
     2821 gcgaagctgc gcggcgatca gatccgcttc agcttcgtcg acatgcaggg cgtcacccac
     2881 gaattcgccg ggcgcgtgct acccgggcgc atggagggca ccgtcaccac ctccggcgcc
     2941 agcaagccgt ggaccgcgac cccgcggccc gcagcccagc cgtagcccga ctcagcgcgc
     3001 gccggacccg tcggtcggct tgaacgcgag gctcagcgag ttgatgcagt agcgcaggcc
     3061 ggtcggcttc gggccgtcgg ggaacacgtg gccgaggtgg ccgccgcagc gcttgcacag
     3121 cacctcgacg cggcgcatcc cgtgggtggt atcgacgcgc tcctcgatcg cgtcgcgctc
     3181 gagggcctca taaaaactcg gccagccgca gccggcgtcg aacttcgtgg tcgactcgaa
     3241 cagcaccgcg ccgcacccgg cgcaggtgta ctggcccgcc gcggtgtgat tccagtactc
     3301 gcccgtgaac gggcgctccg tgccggcctg gcgcagcacc cggaactggt ccggggtcag
     3361 cacgccacgc cactcctcgt cgctcttttg aaccgtccgc tcgctcatgg cgcccagtgt
     3421 aactgcaagg ccgggggtcg gccacgcgcc tccatggtcg gcgagcggcc cgctggcgag
     3481 cgcttgccgc cgacgtcgac gcgctgcgac cctgacagcg ctgtcacggc ccgccgccga
     3541 tgtcggctca cgtctcaccg gccgcgttga gatcgttgca cttctcgggt ggtcgagaac
     3601 ttgaagaagc ggcggcacaa tgcttaggcc gatcctgctg ctcgcctgtc cgatcctcct
     3661 gctctccgcg tgtaccgtgc aggtcgacgc cgaccccgaa gtcgacgccg gcaccaccga
     3721 cgccgaggag agcgagggcc acagcaccca gggccccgac gacgcgacga ccgggcccgc
     3781 gcccaccacc gagcccgagg ccacgtcctc gacgagcacc acgaccccgg ccgacccgac
     3841 cggcgagccg atcgacgacc ccgccaacga tggttgcgtc ccgcaggacg acgtcatcgc
     3901 cgtgcacgag gcggtgttag attcgagcac cggggagccc gacccgtcga gcagcagctc
     3961 cggtggcacc gccggcgaca ccgagggcga caccgacgcc gagggcgaca ccgacttcga
     4021 aagtgacacc gacggcgccg aggagtgcgc gcccctgccg tcgcatacgc cctcgaagct
     4081 cgacaccaat tgcaagtgcg acattcagaa ggagcatcgc gcctgcaaga cccccgatgc
     4141 gcaggccggc acccgctatt gtgatggtgg cgagtggggc ccctgcatcg tcgagccggc
     4201 gtgcgacccc ggctccttta tcgtctgtta ttactgcgac gagcagttcg gcaccatgct
     4261 gagcgattgc ttgctgcacg acggcgtgcc ggtcgacaac tccggggaag actgcgcgac
     4321 gccgctggtg ctcagcttcg acggtcgcgc ggtcgagtac accagcgcgc acgccgagtt
     4381 cgcgatggcc gacggcgacg gctgcggcgc gcgcgactgg ccgacggccg cgaccccgtg
     4441 gctggcgatc gacctcgacc gcaacggcaa catcgacggc ggtcacgagc tgttcggcac
     4501 cggctcacgc ctggccggtc gcgtcgacaa cggcttcgcc gcgctcgcgg ccctcgactc
     4561 caacgcggac ggccagatct ccggcgccga cgcgcgcttc gccgagatcg tcgtatggtc
     4621 ggaccacgac gccgaccgcc gctcgacgat gtgggagctg cagccgctgg ccagtcacgg
     4681 cgtggtggcg atcgacctcg gctacgcgtt cgacgccagc cgctgcgacg cgcgcggcaa
     4741 ctgcgagggc gagcgcgcca gcttcgtgtt cgagacggcg gacggggccc gcagctccgg
     4801 cgagatcgtc gacgtgcgcc tgtcgtgcca gtgatcggcg cgcgagctca gcgcccgacg
     4861 aagctccggg agcgatcgcc gtggatcacg gcgcgctccc ggagctctct cggtcgtgac
     4921 accggcggcc gacatcgtgt ccgccgcgcg gacatcacgc tcagttgcag gtgatcgacg
     4981 tgatctgctg gccgtcgttg caggtgtggt agtcgaggat ctggttgtac cagggcccgt
     5041 cctgccagta tatcgagcgg tgatatgga
//
LOCUS       DASWNJ010001927         1800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_493210, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001927 DASWNJ010000000
VERSION     DASWNJ010001927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493210"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VGB85_32160"
     CDS             complement(<1..266)
                     /locus_tag="VGB85_32160"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY0138799.1"
                     /translation="MSLLWAIRHAPVQAVGLCYGRSDVETAIGHDTAAARVLAYGPWS
                     LDRVWSSPSARCHEPAARIAAALAVPLVIDERLYELDFGAWEGLT"
     gene            complement(263..1027)
                     /locus_tag="VGB85_32165"
     CDS             complement(263..1027)
                     /locus_tag="VGB85_32165"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEY0138800.1"
                     /translation="MTIALRGLRAAITFLTRVPVGGFPYSDAEWRWAAGWFPAVGLLV
                     GCACAAVDLLLLPAGPWPAAIASVIAGLLLTGAFHEDGLADTADAMGGAYTRERLLEI
                     LKDSRVGTFGAAALTMALLLRVACLAALGPAAPAVIVVVHACSRVPPLWLMRALPYVT
                     RDDAARSRLVARPGDPQVLLATATGALVLLAAVGLGALSSGQALALTGVGIGAAIVCG
                     WRFVARAGGLTGDFLGATQQVVEGLGLLALLRVAAA"
     gene            complement(1062..>1800)
                     /locus_tag="VGB85_32170"
     CDS             complement(1062..>1800)
                     /locus_tag="VGB85_32170"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF014347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEY0138801.1"
                     /translation="TIPARPPAGGTEVVRRELGGEVGDLLHTDAMDPALYAAALRAGA
                     DEVDAQGPALRVLVLGEMGIGNSTAAAAVYAALLGGDPAQYGGPGTGVQGEALARKRR
                     LLRTAVERALHAHPGLQDDPHRALQTLGGRDIAAITGAMARAISRGVAVLVDGYIVGA
                     AALALCRLDPGWRAGMIFAHRSAEPAHARALAAMQATPLLDLGMRLGEASGALVAFPL
                     LEAACVLHAEMATFREAQVPDREDMSQ"
BASE COUNT          241 a          660 c          719 g          180 t
ORIGIN      
        1 gtgaggccct cccaggcgcc gaagtcgagc tcgtacaggc gctcgtcgat caccagcggg
       61 accgcgagcg ccgcggcgat gcgggcggcg ggttcgtgac agcgggcgct cggcgagctc
      121 cacacgcgat cgagggacca gggcccgtac gcgagcacgc gggcggcggc ggtgtcgtgg
      181 ccgatcgcgg tctcgacgtc gctgcggccg taacacagcc ccacggcctg caccggcgcg
      241 tggcggatcg cccacagcag gctcacgcgg cggccacccg cagcagcgcg agcagcccga
      301 ggccctcgac gacctgctgg gtggcgccga ggaagtcgcc ggtgaggccg ccagcgcggg
      361 cgacgaagcg ccagccgcac acgatcgccg cgccgatccc gacgccggtg agcgcgaggg
      421 cctgcccgct cgacagcgcg ccgaggccga cggcggccag caggacgagc gcgccggtgg
      481 cggtggccag cagcacctgc ggatctccgg gccgggcgac caggcggctg cgcgcggcgt
      541 cgtcgcgggt gacgtagggc agcgcgcgca tcagccacag cggcggcacg cgcgagcagg
      601 cgtgcacgac gacgatcacc gccggcgcgg cgggcccgag cgcggcgaga caggcgacgc
      661 gcagcagcag cgccatcgtc agcgcggcgg cgccgaacgt gccgacgcgg ctgtccttga
      721 ggatctccag caggcgctcg cgcgtgtagg cgccgcccat cgcgtccgcg gtgtcggcga
      781 ggccgtcctc gtggaaggcg ccggtcagca gcaggccggc gatcacgctg gcgatcgccg
      841 ccggccacgg acccgcgggc agcaacaaca ggtcgaccgc cgcgcaggcg cagccgacca
      901 gcaggccgac cgccgggaac cacccggcgg cccagcgcca ctcggcgtcg ctgtacggga
      961 agccgccgac cggcacgcgg gtcaggaagg tgatggcggc gcgtagaccc cgcagcgcga
     1021 tcgtcatggc tgtcctcttc gccatgtccc cggggaccgg ttcattggga catgtcctcg
     1081 cggtcaggta cctgggcctc gcggaaggtc gccatctcgg cgtgcaggac gcaggcggcc
     1141 tcgagcaggg ggaaggcgac gagcgcgccg ctggcctcgc cgaggcgcat gccgaggtcg
     1201 agcagcgggg tcgcctgcat cgccgcgagg gcgcgcgcgt gggcgggctc ggccgagcgg
     1261 tgggcgaaga tcatgccggc gcgccagccc gggtcgaggc ggcacagcgc gagcgcggcg
     1321 gcgccgacga tgtaaccgtc gacgagcacg gcgacgccgc gggagatcgc ccgcgccatc
     1381 gcgccggtga tcgcggcgat gtcgcgaccc ccgagcgtct gcagggcgcg gtgcgggtcg
     1441 tcttgcagcc cggggtgcgc gtggagggcc cgctcgacgg cggttcgcag cagccggcgc
     1501 ttgcgcgcga gcgcctcgcc ctgcacgccg gtgcccggtc cgccgtactg cgccggatcg
     1561 ccgccgagca gcgcggcgta cacggccgcg gccgcggtgc tgttgccgat gcccatctcg
     1621 ccgagcacga gcacacgcag ggccgggccc tgcgcgtcga cctcgtcggc gccggcgcgc
     1681 agggccgcgg cgtagagggc agggtccatc gcatcggtgt gcagcaggtc gccgacctcg
     1741 ccgccgagct cgcggcgcac gacctcggtg ccgcccgcgg gcggtcgagc ggggatcgtg
//
LOCUS       DASWNJ010001928         2266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_281671, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001928 DASWNJ010000000
VERSION     DASWNJ010001928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281671"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VGB85_32175"
     CDS             complement(<1..506)
                     /locus_tag="VGB85_32175"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0138802.1"
                     /translation="MSVAYVLARGAVLSACAALLFGCLDATPGFTGAPTSSTSDASTG
                     EDPSQTTGGVPTTGGDASTGEASDTGIATTDVPGTSTGLEDTTGEPPMQCGDGVVDED
                     EFCDDGDDNDGDECPSSCAHAFCGDGYVWTGIEDCDDGNKINDDACTNACTMALCGDG
                     VTHSDVED"
     gene            588..1025
                     /locus_tag="VGB85_32180"
     CDS             588..1025
                     /locus_tag="VGB85_32180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEY0138803.1"
                     /translation="MIRVPPCELDLPAPGEVCEVTIEYPRWSMVKRRSDGSLDFVAPL
                     PCPYNYGSISGRRSGDGDLLDAVVLGPRLPAGTRRSLPAHGVVGFLDLGVHDPKVICS
                     AAPMTARERAGLELFFRSYAGFKRALAFARGRHEPTRYLGWLR"
     gene            1130..1702
                     /locus_tag="VGB85_32185"
     CDS             1130..1702
                     /locus_tag="VGB85_32185"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HEY0138804.1"
                     /translation="MLARITLAMSLLLACKPATAPSADTARPEPAAAPVVARPTAVFV
                     VRHGEKADDDPRDPTLSPAGQARAGALATTLAHVGVTHLFTSEFRRTQATLRPLADAA
                     KLEPVIIPAADQAALVSAIEALPAGSIVVVAGHSNTVPALVTALGGEARGTVASPAGP
                     VLPDVAYDRLFLVIRSATGEVQTLELRQAH"
     gene            complement(1696..>2266)
                     /locus_tag="VGB85_32190"
     CDS             complement(1696..>2266)
                     /locus_tag="VGB85_32190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138805.1"
                     /translation="APGLVEVLARCLQRAPERRFASAQALSDALAPFERTGERMTLTP
                     VTPEPRASARAIAAMPATPARPVRAPGLPAPSRAPSGSHVPVPAGPSGSHRVVPSSGP
                     LSEARPVGALPVPRDMKRTGPLVLPPELKRSGIQAVPKDIGRGAAPVQAAARVVPARQ
                     APGDAYAWLWGVLLALLGGGLVLIAVLRQ"
BASE COUNT          269 a          899 c          787 g          311 t
ORIGIN      
        1 cagtcctcga cgtcgctgtg cgtcacgccg tcgccgcaca gcgccatcgt gcacgcgttg
       61 gtgcacgcgt cgtcgttgat cttgttgccg tcgtcgcagt cctcgatgcc ggtccacacg
      121 tagccgtcgc cgcagaaggc atgcgcgcag ctgctcgggc attcgtcgcc gtcgttgtcg
      181 tcgccgtcgt cgcagaactc gtcctcgtcg accaccccgt cgccgcactg catcggcggc
      241 tcgcccgtgg tgtcttcgag gcccgtgctg gtgcccggca cgtccgtggt cgcgatcccg
      301 gtgtcgctcg cctcgccggt gctcgcgtcc ccgccggtcg tcggtacgcc gccggtcgtc
      361 tgcgacgggt cctcaccggt gctcgcgtcc gaggtcgagg aagtgggcgc gccggtgaag
      421 cccggggtcg catcgagaca gccgaaaagc agcgccgcgc aggcggagag gacggcgccg
      481 cgcgcgagga cataagccac agacatggac gcaggttctc atggagtccc gcgaggcgac
      541 agacggcgcg gtcgacgtgc ggcgcctcgc gtgttagacg ctcgcgtgtg atccgcgtgc
      601 caccttgcga gctcgacctg ccggcccccg gagaggtctg cgaggtgacg atcgaatacc
      661 cgcggtggag catggtgaag cgccgcagcg acggctcgct cgacttcgtc gcgcccctgc
      721 cctgccccta caattacggg tcgatctccg ggcgccgctc cggcgacggc gacctgctcg
      781 acgccgtcgt gctcggcccg cgcctgcccg ccggcacccg gcgctcgctg ccggcgcacg
      841 gcgtcgtcgg cttcctcgac ctcggcgtcc acgaccccaa ggtcatctgc agcgccgcgc
      901 cgatgaccgc gcgcgaacgc gcgggcctcg agctgttctt tcgcagctac gccggcttca
      961 agcgggccct cgcgttcgcc cgcggccgcc acgagccgac ccgctacctc ggctggttgc
     1021 gatgatgttc ctccaccccg ccggtggatc tccacacctg tcctccggga caggcgtacg
     1081 cgggcacgcg cgaaacctcc cctcgccggt ccgggtccga gcgtctccca tgctcgctcg
     1141 catcacgctc gccatgtcgc tcctgctcgc ctgcaagccg gccaccgcgc cgtccgccga
     1201 caccgcgcgg cccgagcccg ccgccgcgcc ggtcgtcgct cgaccgaccg ccgtgttcgt
     1261 cgtgcgtcac ggcgagaagg ccgacgacga cccgcgcgac ccgaccctca gccccgccgg
     1321 ccaggcacgc gccggggccc tcgctaccac gctggcgcac gtcggcgtca cgcacctgtt
     1381 caccagcgag ttccgccgca cgcaggcgac gctgcgtccg ctcgccgatg ctgcgaagct
     1441 cgagccggtg atcatccccg cggcggacca ggccgcgctg gtgtcggcga tcgaggcgtt
     1501 gccggccggc tcgatcgtcg tcgtcgccgg gcactccaac accgtgcccg cgctggtgac
     1561 cgcgctcggc ggcgaggcga ggggcacggt cgccagcccg gccggccccg tgctgcccga
     1621 cgtcgcctac gaccgcctgt ttctcgtcat ccgttcggcg accggcgagg tgcagacgct
     1681 cgagcttcgc caggctcact gacgcagcac ggcgatcagc accagcccgc cgccgagcag
     1741 cgccagcagc acgccccaca gccacgcgta cgcgtccccc ggggcctgcc gcgccggcac
     1801 gacccgcgcg gccgcctgta ccggcgccgc gccgcgcccg atgtccttcg ggacagcctg
     1861 gatcccgctg cgcttcagct ccggcgggag caccagcggg cccgtgcgct tcatgtccct
     1921 ggggacaggt agcgcgccca cgggtctcgc ctcgctgagc ggcccggacg acggcaccac
     1981 gcggtgcgac cccgacggcc cggcgggcac cggcacgtgc gatcccgagg gcgcgcgcga
     2041 cggggcgggc aggcccgggg cacgcacggg gcgggccggc gtggcaggca tcgccgcgat
     2101 cgcccgcgcc gacgcgcgcg gctcgggtgt gacaggcgtg agggtcatgc gctcgccggt
     2161 ccgctcgaac ggcgcgagcg cgtcgctgag cgcctgggcc gacgcgaagc gccgctcggg
     2221 cgcgcgttgc aggcagcgcg cgagcacctc gacgagccct ggcgcg
//
LOCUS       DASWNJ010001929         1502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_69227, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001929 DASWNJ010000000
VERSION     DASWNJ010001929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69227"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="VGB85_32195"
     CDS             complement(<1..1051)
                     /locus_tag="VGB85_32195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138806.1"
                     /translation="MIINRAHPLMLTAILASCPGPEGQPDPNATDPTSTGGPQDTSST
                     SGESTDTPTTDTPTTDGSATGGTSTGDPTTDDTTTGTTDPVVTRVLYHPFVEILGSPG
                     ASAARYVEIVDGVAMPPITIVDPPAEQLNTSDHTADRPWSPYYSNANDAPQLWLVDLE
                     TLTPHEITLPAEVTRVLTARVSRGDSHLIVWARPPGDAPAPEDYQYYVCELGPAAQCA
                     IEPVEAATGPTTYVADIHDISATSGRIWYTTQEIGGTGQTVLQGDVAAPEAAITLAIF
                     PDGGLLSRISLDEKTAYFRSADGDQLQALDISVDPPGSLVPLHPPQPGMHWSWSDDEK
                     DLLIYVEDGKYGDLFH"
     gene            1243..>1502
                     /locus_tag="VGB85_32200"
     CDS             1243..>1502
                     /locus_tag="VGB85_32200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138807.1"
                     /translation="MVKGRYRWSARVLGAWLLGGCGDDGGAGSTSQASETTAVDSSTS
                     GPGTEPTTGPTTGPSTATSGDDGTGSTGEPTTDPTTGGEPACT"
BASE COUNT          204 a          456 c          606 g          236 t
ORIGIN      
        1 cgtggaacag atcgccatat ttgccgtcct cgacgtagat caggagatcc ttttcgtcgt
       61 cggaccagct ccagtgcatg cccggctggg gcgggtggag cgggaccagc gagccgggcg
      121 ggtcgacgct gatgtccagc gcctggagct ggtcaccgtc ggcggacctg aaataggcgg
      181 tcttctcgtc gagcgagatc ctgctgagga ggccgccgtc ggggaagatg gccagcgtga
      241 tcgcggcctc gggagcggcg acgtcgccct gcagcacggt ctgcccggtt ccgccgatct
      301 cctgggtcgt gtaccagatc cgtccgctcg tcgccgagat gtcgtggatg tcggcgacgt
      361 aggtcgtcgg cccggtcgcg gcctcgaccg gctcgatcgc gcattgtgcc gcgggtccga
      421 gctcgcatac gtaatactga tagtcctcgg gcgccggtgc gtcgccgggc gggcgggccc
      481 acacgatcag gtggctgtcc ccgcgtgaca cgcgggccgt cagcacgcgg gtgacctccg
      541 ccggcagcgt gatctcgtgc ggcgtcaggg tctccaggtc gaccagccac agctgcggcg
      601 cgtcgttcgc gttgctgtaa tagggcgacc aggggcggtc ggcggtatgg tcgctggtgt
      661 tgagctgctc ggccgggggg tcgacgatgg tgatcggcgg catcgccacg ccgtcgacga
      721 tctcgacgta gcgggccgcg gacgcgcccg gcgagccgag aatctccacg aaggggtgat
      781 acaggacccg cgtcacgacg ggatcggtgg tgccggtggt cgtatcgtcg gtcgtcggat
      841 cgccggtgct cgtaccgccg gtggccgagc cgtcggtggt cggcgtatcg gtggtcggtg
      901 tatcggtgga ctcgccggag gtgctcgagg tgtcctgcgg gccgccggtg ctcgtggggt
      961 cggtcgcgtt cggatccggc tgaccttccg ggcccgggca ggaggcgagg atggccgtca
     1021 acatcagcgg gtgggctcga ttaatgatca tcgcggcagg gtacacgagc gctgcagcga
     1081 ggtggcgtgt gtgatgcgtt ccacaaagcg cgccagaacg gcgtcgcatc gcgtgtgggc
     1141 cgtgcacccc gggtgcgcgg cgcgatgtcc atcgctcgcg gcgctgcggc gcagtggccc
     1201 cgcgcgagca aagctgcttg gatcgcacgg gaggtgcggg ccatggtgaa ggggcgatat
     1261 cggtggagcg cgcgggtgct cggggcgtgg ctgctcggcg ggtgtggtga cgatggcggg
     1321 gctggatcga cgtcgcaggc gagcgagacg acggcggtcg acagctcgac gagcgggccc
     1381 gggaccgagc cgacgacggg gccgacgacg ggcccgagca cggcgacgag cggcgacgac
     1441 gggacgggga gcacgggcga gccgacgacc gatccgacga ccggcggcga gccggcgtgc
     1501 ac
//
LOCUS       DASWNJ010001930         2104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_493248, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001930 DASWNJ010000000
VERSION     DASWNJ010001930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493248"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2104
                     /gene="secA"
                     /locus_tag="VGB85_32205"
     CDS             <1..>2104
                     /gene="secA"
                     /locus_tag="VGB85_32205"
                     /inference="COORDINATES: protein motif:HMM:NF009538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY0138808.1"
                     /translation="VHRFLGLSVGVVLPGERPDQRRGQYACDITYGTNNEFGFDYLRD
                     NMAWQKADLVQRGHNFAIVDEADSILIDEARTPLIISGPAEQSARWYQEFARMSPLLQ
                     RDVHYEVDERKRTVGVTEEGVTLVEDQLGIDNLYEAANTPLVGYLNNAIKVKELFKRD
                     KDYIVKNGQVLIVDEFTGRVLAGRRYNEGLHQAIEAKEGVEVQAENQTLATITLQNYF
                     RLYTKLSGMTGTAQTEAAELHQIYKMHVVPIPTNRPMVRADQADLIYKTEEAKFAAVA
                     ADIAEKHGKGQPVLVGTTSVEKSEYLSKLLLGLQVPHEVLNAKQHEREAHIIAEAGRA
                     PARHGDDKRTGAVTVATNMAGRGTDIVLGGNPEFLADEELRRRGLDPVETREEYEAAW
                     DDTVAAMKDQVAAEAEEVKAAGGLYVLGTERHESRRIDNQLRGRSGRQGDPGESRFYL
                     SLGDELMRRFNGQLVESVMNRFNLPDDVPIEAGMVTKAIRSAQTQVEQQNFEIRKNVL
                     KYDEVLNKQRMVIYAERRRVLAGEDIGEQIEHMLGDVVGAYVDGATAEGYAEDWDLDK
                     LWTALGQLYPVTLDRKELTDGDHADDLTREALTEAVLADARAAYAVREAEIDAVIGAQ
                     GGMRELERQVLLQVMDRKWREHLYEMDYLKEGIGLRAMAQRDPLIEYQREGFDMFAAM
                     LEGIKEETVGHLFNVRVQA"
BASE COUNT          395 a          719 c          733 g          257 t
ORIGIN      
        1 ggtccaccgc ttcctcggcc tgagcgtcgg ggtcgtcctg ccgggcgagc ggccggacca
       61 gcgccggggg cagtacgcct gcgacatcac ctacggcacg aacaacgagt tcggcttcga
      121 ctacctgcgc gacaacatgg cgtggcagaa ggccgatctc gtgcagcgcg gccacaactt
      181 cgcgatcgtc gacgaggcgg actcgatcct catcgacgag gcccgcaccc cgttgatcat
      241 ctcggggccg gcggagcaga gcgcccggtg gtaccaggag ttcgcgcgga tgagcccgct
      301 gctccagcgc gacgtccact acgaggtcga cgagcgcaag cgcacggtcg gcgtcaccga
      361 ggagggcgtc acgctggtcg aggaccagct gggcatcgac aacctctacg aggcggcgaa
      421 caccccgctg gtcggctacc tgaacaacgc gatcaaggtc aaggagctgt tcaagcgcga
      481 caaggactac atcgtcaaga acggccaggt gctcatcgtc gacgagttca ccggccgcgt
      541 gctcgccggc cgtcgctaca acgagggcct gcaccaggcg atcgaggcca aggagggcgt
      601 ggaggtccag gccgagaacc agaccctggc caccatcacg ctgcagaact acttccggct
      661 ctacaccaag ctctcgggca tgaccggcac ggcgcagacc gaggcggccg agctgcacca
      721 gatctacaag atgcacgtgg tgccgatccc gacgaaccgg ccgatggtcc gcgccgacca
      781 ggccgacctc atctacaaga ccgaggaggc gaagttcgcg gccgtcgccg ccgacatcgc
      841 cgagaagcac ggcaagggcc agccggtgct ggtcggcacc accagcgtgg agaagtcgga
      901 gtacctgtcc aagctgctcc tcggcctgca ggtgccgcac gaggtcctca acgccaagca
      961 gcacgagcgc gaggcgcaca tcatcgcgga ggccggccgg gcaccggcgc gccacggcga
     1021 cgacaagcgc accggcgccg tcaccgtcgc caccaacatg gccggccgcg gcacggacat
     1081 cgtgctcggc ggcaaccccg agttcctcgc cgacgaggag ctgcgccggc gcggcctgga
     1141 ccccgtcgag acccgcgagg agtacgaggc ggcctgggac gacaccgtgg cggccatgaa
     1201 ggaccaggtc gcggccgagg ccgaggaggt caaggccgcg ggcgggctgt acgtgctcgg
     1261 caccgaacgc cacgagtcgc gccggatcga caaccagctc cgcggccgct cgggtcgcca
     1321 gggcgacccc ggcgagtccc ggttctacct ctcgctcggc gacgagctga tgcgccggtt
     1381 caacggccag ctcgtcgagt cggtcatgaa ccggttcaac ctgccggacg acgtgccgat
     1441 cgaggccgga atggtcacca aggccatccg cagcgcgcag acgcaggtcg agcagcagaa
     1501 cttcgagatc cgcaagaacg tcctcaagta cgacgaggtg ctcaacaagc agcgcatggt
     1561 catctacgcc gagcgccgcc gggttctcgc cggcgaggac atcggcgagc agatcgagca
     1621 catgctcggt gacgtggtcg gcgcctacgt cgacggtgcg accgccgagg gctacgcgga
     1681 ggactgggac ctcgacaagc tctggacggc gctcggccag ctctacccgg tcacgctgga
     1741 ccgcaaggag ctcaccgacg gcgaccacgc cgacgacctg acccgcgagg cgctgaccga
     1801 ggcggtgctg gccgacgccc gggcggccta cgcggtccgc gaggccgaga tcgacgcggt
     1861 gatcggcgcg cagggcggca tgcgcgagct ggagcgccag gtgctgctgc aggtcatgga
     1921 ccgcaagtgg cgcgagcacc tctacgagat ggactacctc aaggagggca tcgggctgcg
     1981 ggcgatggcc cagcgcgacc cgctgatcga gtaccagcgt gagggcttcg acatgttcgc
     2041 cgcgatgctc gagggcatca aggaggagac ggtcgggcac ctgttcaacg tgcgggtcca
     2101 ggcg
//
LOCUS       DASWNJ010001931         3068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_416494, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001931 DASWNJ010000000
VERSION     DASWNJ010001931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416494"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VGB85_32210"
     CDS             complement(<1..366)
                     /locus_tag="VGB85_32210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138809.1"
                     /translation="MSYLRLNYAQIAALAALLTACTDKTETSESTSGTDTSTTDASTT
                     DGTGTDGTPTTTGDTSTGGQPSDPFGFVCVSLQLGESVDDDPFVGTRKVEVTLNYEPC
                     LVAYYTNKHPEMRQDGQDGA"
     gene            complement(428..1825)
                     /gene="murF"
                     /locus_tag="VGB85_32215"
     CDS             complement(428..1825)
                     /gene="murF"
                     /locus_tag="VGB85_32215"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEY0138810.1"
                     /translation="MHWTPETLAAAAGGRLLRRSERTIAGAFIDSRVPVVGGLFVPIV
                     AARDGHDFIPAALAGGASALLVRPGHAPAGGDCTVVEVEDTLLALQRLARDARARCAG
                     PVVAITGSNGKTTTRAMIDAVLRTRFERVLATRGNFNNHLGVPLTLLGEPEDPQAAVL
                     ELGMSAPGENDLLGAIVRPQVAVITSIALEHLEFMGSLEAIAAAEAEVVRHLQPGGVV
                     VLPGDEPLLLPHVGDVRALRFGEGPDTAVQIVGVEQDERTHVTLRLHHRAGGEEVRLG
                     LSVFGAHNARNAAAALAVGLELGCPVPAMLEALEAILPVGDRGRVLRFGPHLLIADCY
                     NANPGSMAAALRSLAGLRGVRTGPLVAVLGDMLELGETAPQLHADLGALAADLGLDAI
                     FTFGELSQHASAVARARGIAAWHAGDSVDDAVAAVRARFDTRPGALLIKGSRGMRLER
                     VVEALTEAPVVVEGS"
     gene            1981..2487
                     /locus_tag="VGB85_32220"
     CDS             1981..2487
                     /locus_tag="VGB85_32220"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY0138811.1"
                     /translation="MKTLVLDQGYVPVKVITWRRALCMSLIEKVEVLASYDREVRTVS
                     RSFRAPAVIRLLQTVRTRPQLVRFSRRNVYLRDNHRCQYCGLTFAPAELTLDHVVPRV
                     FGGKTSWTNVVAACGSCNRRKGGRTPEQAGLALRTHPRRPQWSPMSVTQRLPDTDVPE
                     IWRAWIFT"
     gene            complement(2500..>3068)
                     /locus_tag="VGB85_32225"
     CDS             complement(2500..>3068)
                     /locus_tag="VGB85_32225"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY0138812.1"
                     /translation="WFTLGNAFCGVGAVFAAMRFLQDGHPGDLLRGMALMPLAFMFDA
                     LDGRIARWRHSASTLGRELDSLADVISFGVAPAALAYACGMQGGWDWIVLSYFLGCGV
                     SRLARYNVTAESLSGDDGKVRHFEGTPITTSLALVVVLALATWQGRIGGSLWFGVVDL
                     GWELHPLVLMFALSGSLMISKTLHIPKP"
BASE COUNT          443 a         1179 c         1031 g          415 t
ORIGIN      
        1 ggcgccgtcc tggccgtcct ggcgcatctc cgggtgcttg ttcgtgtagt aggcgaccag
       61 gcacggctcg tagttgagcg tgacctcgac cttgcgcgtg ccgacgaacg ggtcgtcgtc
      121 gacggactcg ccgagttgca ggctgacgca gacgaagccg aacgggtcgg acggctgccc
      181 accggtgctg gtgtcgccgg tcgtcgtcgg cgtgccatcg gtgccggtgc catcggtggt
      241 cgaggcatcg gtggtcgagg tgtcggtgcc cgacgtgctc tcgctggtct cggtcttgtc
      301 ggtgcaggcg gtcagcaggg ccgcgagggc ggcgatctgc gcgtaattca ggcgtaagta
      361 cgacatggcc ggggaggcta gcagcttccc cggccgtggt cggtcggctt tttcagcgcg
      421 cgagttgcta cgacccctcg acgaccacgg gcgcctcggt cagcgcctcg acgacccgct
      481 cgagccgcat cccgcggctg cctttgatca gcagggcccc gggccgcgtg tcgaagcgag
      541 cgcgcaccgc cgcgaccgcg tcatcgacgc tgtcacccgc gtgccacgcg gcgatcccac
      601 gcgcgcgcgc gaccgccgag gcgtgctgcg acagctcccc gaacgtgaag atcgcgtcga
      661 gcccgaggtc ggccgcgagc gccccgaggt ccgcgtgcaa ttgcggcgcc gtctcgccga
      721 gctccaacat gtccccgagg acagccacga gcggcccggt cctcaccccg cgcagacccg
      781 cgaggctgcg cagcgccgcc gccatcgagc ccgggttggc gttgtaacag tcggcgatca
      841 gcaggtgcgg gccgaagcgc agcacccgtc cgcggtctcc gaccggcaag atcgcctcga
      901 gcgcctcgag catcgccggc accggacagc cgagctcgag cccgaccgcc aacgcggccg
      961 ccgcgttgcg ggcgttgtgc gccccgaaca ccgacagccc gagccgcacc tcctcgccgc
     1021 cggcgcgatg atgcaggcgc agcgtcacgt gggtgcgctc gtcctgctcg accccgacga
     1081 tctggaccgc cgtgtccgga ccctcgccga agcgcagcgc ccgcacgtcg ccgacgtgcg
     1141 gcagcagcag cggctcgtcg cccgggagca cgacgacccc gcccggctgc aggtggcgca
     1201 cgacctcggc ctcggccgcc gcgatcgcct cgagcgagcc catgaactcg aggtgctcga
     1261 gcgcgatgct cgtgatcacc gcgacctgcg gccgcacgat cgcgccgagc aggtcgttct
     1321 cccctggcgc gctcatgccc agctcgagca cggccgcctg cgggtcctcc ggctcgccga
     1381 gcagcgtcag cggcaccccg aggtgattat tgaaattgcc ccgggtcgcc agcacccgct
     1441 cgaagcgagt ccgcagcacc gcgtcgatca tcgcccgcgt cgtcgtcttg ccgttgctcc
     1501 cggtgatcgc caccaccggc ccggcgcagc gggcccgggc gtcgcgcgcc aggcgttgca
     1561 gcgccagcag cgtgtcctcg acctcgacca cggtgcagtc accgccggcc ggcgcgtgcc
     1621 ccggccggac cagcagcgcc gaggccccgc ccgcgagcgc ggccgggatg aagtcatgtc
     1681 cgtcacgcgc agcgacgatc ggcacgaata atccccccac caccggcacc cggctgtcga
     1741 tgaaggcgcc ggcgatcgtt cgctcgctgc gccgcagcag gcgtccgccg gcagcggcgg
     1801 cgagggtctc gggggtccag tgcacgtccc cggataccaa actccacgcc cggtccgcca
     1861 gtcacccccg ccagatcggg gccgggcgcg tctacgagcc ggggaccgcg gccggacccg
     1921 cccgttgcgt tctccggcgc cgtgctagcc ttggagctct gcgcacggga gggaggttcc
     1981 atgaaaacat tggtcctcga tcagggctat gtgccggtga aggtcatcac ctggcgccgc
     2041 gccctgtgca tgagcctgat cgagaaggtc gaggtcctcg ccagctatga ccgcgaggtc
     2101 cgcaccgtct cccggtcctt tcgcgccccc gccgtcatcc gcctgctcca gaccgtccgc
     2161 acccgccccc agctggtccg cttctcgcgc cgcaacgtct acctgcgcga caaccaccgc
     2221 tgccagtact gcggcctcac cttcgccccc gccgagctga ccctcgacca cgtcgtgccc
     2281 cgcgtgttcg gcggcaagac ctcgtggacc aacgtcgtcg ccgcctgcgg cagctgcaac
     2341 cgccgcaagg gcggccgcac ccccgagcag gccggcctcg ccctgcgcac ccaccctcgc
     2401 cgcccgcagt ggtcgccgat gtcggtcacg cagcgcctcc ccgacaccga cgtcccggag
     2461 atctggcgcg cctggatctt cacctgagcc gcggcggcct cagggtttgg ggatatgcag
     2521 ggtcttgctg atcatcaggg accccgacag cgcgaacatc aggaccagcg ggtgcagctc
     2581 ccagccgagg tcgaccacgc cgaaccataa cgagccgccg atgcgcccct gccaggtggc
     2641 cagcgccagc acgacgacga gcgccaggct cgtcgtgatc ggcgtcccct cgaagtggcg
     2701 gaccttgccg tcgtcgccgg acagcgactc ggccgtgacg ttgtagcggg ccagacggct
     2761 gacgccgcag cccaggaagt acgacaggac gatccagtcc cacccgccct gcatgccgca
     2821 ggcgtacgcc aacgcggccg gcgcgacgcc gaaggagatc acgtccgcga gcgagtcgag
     2881 ctcgcggccg agggtcgacg cgctgtgccg ccagcgggcg atccgtccgt cgagcgcgtc
     2941 gaacatgaac gccagcggca tcagggccat gccgcgcagc aggtcgccag ggtgcccgtc
     3001 ctgcaggaag cgcatcgcgg cgaacacggc cccgacccca caaaaggcgt tccccagcgt
     3061 gaaccagt
//
LOCUS       DASWNJ010001932         4107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_33988, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001932 DASWNJ010000000
VERSION     DASWNJ010001932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33988"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VGB85_32230"
     CDS             complement(<1..687)
                     /locus_tag="VGB85_32230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138813.1"
                     /translation="MSEKIWEELLIWKISGARYDEHKGVELADLSGLSALRHALVELT
                     KALWKRRNDRKRIPDLVEEKIDLRIQRFEDGCQQTTISLGRPRTVVPIPIQGALFPPP
                     PDDENSFFDTLHDAAELLEDFLADLKGDDQPRMPSWVPREVYQQLLLVGGKRRTGEEV
                     SIKALRWRSHDPPQVADYDQGEGEGEDEGEGEGEDEGEDEVPPLRTAAKVFSRPPNST
                     RPEPPQIAADV"
     gene            complement(849..2444)
                     /locus_tag="VGB85_32235"
     CDS             complement(849..2444)
                     /locus_tag="VGB85_32235"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY0138814.1"
                     /translation="MLTTARVRELKPQEKMYEVTCEAVAGFVVRVLPSGKKVFLVRRQ
                     FDGKYRREKIGVLGPSLGVDEARRSALAMMDGSERPEPTVQPAARRTAPRPTRQVEPV
                     KQVEPRRVTVRELADRFVREYVDVYLKPGTAAHYRKHLADHILPVLGDRDFESVTRND
                     VQALHASLKHIKGSANYVVSVVGSLYTRIIDDWKLSSMSNPNHKIRMFKMKTRERFLS
                     PEERRQLEEALLRGLQLPPGCKGHLDRMGVWALQLLSLTGLRRDEIRDLTWPMVDWQH
                     SCLNLPDTKTGQRNIPVPSHVIALLRQIHDRTGNRKVGLVVHSRTGRKLSGLNRTWQN
                     IRDAVGIPDVRLHDLRHSFASDALGGGVPLAIVGEMLGHRQPSTTKRYAHLANRVVRE
                     ALEHTAGIIVAATNAPRALSVAPFEPLRDGLWAAIVTLVDADRPRGGKPVELRQVVDG
                     IRWVLHTQAHWSDIPATYAASTTCWRWYKRWCADGTWPKVEALIAAPVAKPARRPASR
                     PQPHASETRQARSHASSRRFASS"
     gene            complement(2553..3716)
                     /locus_tag="VGB85_32240"
     CDS             complement(2553..3716)
                     /locus_tag="VGB85_32240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0138815.1"
                     /translation="MWVALDTGISAETRWDGPSLHDLDDDGLAEVLSASGVYDGATGV
                     RLNPGQVVPGTEPAGIGRIPVAGDLDGDGAVELVGAGVWRWNLGMGVWEMVYPGSPAA
                     IHYGFADFGTPGADPASFDAKTLDGIAEIVTVNGVDVSLYTLGGQLLFTAQQGGGPPT
                     IGDFDNDGYPEISSAGGTAYTVFDLDCKDPATPGCTAPYVRWTQPSQDSSANTGSSIF
                     DFEGDGEAEVVYAEECFVRVYQGSTGEVLYSAFRTSCTWYENAIVGDPDNDQNTEILV
                     GSNNNCGITCPTVDPIHRGSRCEDGTQCAAPNICDSGFCRCEDDTQCLPGNACADPLP
                     DTPGTGKVCRALHPPGVGLTGLRVLRDALDRWASSRPMWNQHAYSITNVIGLV"
     gene            3735..3920
                     /locus_tag="VGB85_32245"
     CDS             3735..3920
                     /locus_tag="VGB85_32245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138816.1"
                     /translation="MIASQPNATTPVTARWLTISGKPSPSRSPIAGGLAATTRFSGSS
                     IVSSCEQSVPFKILTTP"
BASE COUNT          650 a         1327 c         1402 g          728 t
ORIGIN      
        1 gacatcggcc gcgatttgag gcggttctgg gcgagtagag ttaggcgggc gcgaaaatac
       61 cttggccgcc gtccgcaacg ggggtacttc gtcctcgcct tcgtcctcgc cttcgccctc
      121 gccctcgtcc tcgccctcgc cctcgccctg gtcgtaatcg gcaacctgcg gagggtcgtg
      181 actcctccag cgcaatgctt tgatgctgac ctcttcgcca gttcgtcgct tcccgccaac
      241 aagtaagagt tgctgataca cctctcgcgg cacccaggaa ggcatccgcg gttgatcgtc
      301 gcccttgagg tctgctagga agtcctcgag cagttccgct gcgtcatgga gcgtatcaaa
      361 aaaggagttt tcgtcgtcgg gcggcggcgg gaagagagcc ccctggatgg ggataggaac
      421 tactgttcgg ggtctcccga gcgaaatggt ggtttgttga catccgtcct caaacctctg
      481 aatccggaga tcgatttttt cctcgaccag atctggtatc cgctttctat cgttcctgcg
      541 tttccatagg gccttcgtta gctccaccaa ggcatggcga agggcagaca agccgctcag
      601 atcggctagt tcgacgccct tatgctcatc gtaccgcgct ccggaaattt tccagataag
      661 cagttcttcc cagatcttct cggacatgac tccctagcgg aagtctacac ttcaacgtta
      721 ggctatcaaa aaatcgcacc gaacggcgtc ctgcgctgcc ctctacgccg gcgagccgca
      781 cccggcaagc gcgccacaaa tgcccgacga tccggccgcc gccgtgggcg accttccatg
      841 cgggctgcct acgagcttgc gaagcgtcgg ctggatgcgt gggagcgcgc ttgccgggtc
      901 tctgacgcgt ggggctgggg ccgcgacgct ggccgacgcg ccggcttggc caccggcgcc
      961 gcgatcaggg cctcgacctt gggccaggtc ccgtcggcac accaccgctt gtaccagcgc
     1021 cagcatgtcg tggacgccgc gtaggtcgcc gggatatcgg accagtgcgc ctgcgtgtgg
     1081 agcacccagc ggatgccatc gaccacctgt cgcagctcca cgggcttgcc accgcgcggg
     1141 cggtcggcgt ccacaagggt gacgatcgcc gcccacagtc cgtcgcgtag tggctcgaat
     1201 ggcgccacgg acagcgcacg gggcgcgttt gtcgccgcaa cgatgatgcc ggcggtgtgc
     1261 tcgagcgcct cgcggacgac gcggttagcg aggtgggcgt agcgcttggt cgtgctcggt
     1321 tgccggtggc cgagcatctc gccaacgatg gccagcggga caccgccccc gagggcgtcg
     1381 ctggcgaacg agtggcggag gtcgtggagt cggacgtcgg ggatccccac cgcgtcgcgg
     1441 atgttctgcc aggtccgatt caggcccgag agcttccggc ctgtgcgcga gtggaccacg
     1501 agacccacct tgcggttgcc ggtgcggtcg tggatctggc gaagcagggc aatgacgtgc
     1561 gacgggaccg ggatgttgcg ctggcccgtc tttgtgtcgg gcaggttcag gcagctgtgc
     1621 tgccagtcga ccatgggcca ggttaggtcg cggatctcgt cgcggcgcag gccggtgagc
     1681 gacaggagtt gcagggccca gactcccatc cgatcaagat gccccttgca gcccggcggg
     1741 agctgcagac cacgaagcaa ggcctcctcc agctgtcgcc gctcctcggg tgacaggaag
     1801 cgctcgcggg tcttcatctt gaacatccgg atcttgtgat tcgggttgct catgctggac
     1861 agcttccagt cgtcgatgat gcgggtgtac aggctaccca cgacgctgac cacgtagttg
     1921 gccgaaccct tgatgtgctt caggctggcg tgcagcgcct gcacgtcgtt gcgggtcacg
     1981 ctctcgaagt cccgatcgcc cagcacgggc aggatgtgat cggccaagtg cttacggtag
     2041 tgggccgcgg tgccgggctt caggtacacg tcgacatact cgcggacgaa gcggtcggcg
     2101 agctcccgga ctgtcacgcg ccgcggttcc acctgtttca caggctcgac ctggcgcgtc
     2161 ggtctcggcg cggtgcggcg cgcggccggc tggacggtcg gctcgggacg ctcgctgccg
     2221 tccatcatgg cgagcgcact acggcgcgcc tcgtcgacgc caagcgaggg gccgagcacc
     2281 ccgatctttt cgcgccgata cttcccgtcg aactggcgcc ggacgagaaa caccttcttc
     2341 cccgacggca gcacccgtac gacgaagccg gccaccgcct cgcaggtaac ctcgtacatc
     2401 ttctcctgcg gcttgagctc gcgtactcgt gcggtcgtaa gcatcccctg ttcctccacc
     2461 ctcagaatcg cgacaaaatc gagacagaca aggcccaaaa cgagcctgtc cgaaaataca
     2521 cattctcggt tataaagccg cgcagggcgg atctaaacca ggcctatgac attcgtgatg
     2581 gaataggcgt gctgattcca catcgggcgc gaggaggccc agcggtcgag ggcgtcgcgc
     2641 aatacgcgaa ggccggtgag gccgacgccg ggcgggtgca gggcgcggca gaccttgccg
     2701 gtgccgggcg tgtcggggag ggggtcggcg cacgcgttgc cggggaggca ctgggtgtcg
     2761 tcctcgcagc ggcaaaagcc gctgtcgcag atgttcggcg cggcgcactg ggtgccgtcc
     2821 tcgcagcggc tgccgcggtg gatcgggtcg accgtcgggc aggtgatgcc gcagttgttg
     2881 ttgctgccga ccaggatctc ggtgttctga tcgttgtcgg ggtcgccgac gatcgcgttc
     2941 tcgtaccagg tgcacgaggt gcgaaacgcg gagtacagca cctcgccggt gctgccctga
     3001 tagacgcgca cgaagcactc ttcggcgtag accacctcgg cctcgccgtc gccctcgaag
     3061 tcgaagatcg acgagccggt gttggccgat gaatcctgcg agggctgcgt ccagcgcacg
     3121 tacggggcgg tacagccggg cgtcgccggg tccttgcagt cgaggtcgaa cacggtgtac
     3181 gcggtgccgc cggccgacga gatctcgggg tacccatcat tatcgaagtc gccgatcgtc
     3241 ggcgggccgc cgccctgctg ggcggtgaac agcagctggc cgccgagggt gtagaggctg
     3301 acgtcgacgc cgttgacggt cacgatctcg gcgatgccgt cgagggtctt ggcgtcgaag
     3361 ctggccgggt cggcgcccgg ggtgccgaag tcggcgaagc cgtagtggat cgccgcgggc
     3421 gagccgggat agaccatctc ccacacgccc atgccgaggt tccagcgcca cacgccagcg
     3481 ccgacgagct cgaccgcgcc gtcgccgtcg aggtcgccgg cgacggggat cctcccgatg
     3541 ccggcgggtt cggtgccggg cacgacctgg ccggggttga ggcgcacgcc ggtggcacca
     3601 tcatacacgc cggaggccga cagcacctcg gcgaggccgt cgtcgtcgag gtcgtgcagc
     3661 gaggggccgt cccagcgcgt ctcggcgcta atgccggtgt cgagcgcgac ccacacttgg
     3721 tgccatactt cttggtgatc gcgtcccagc cgaacgcgac gacgccggtg acggcgcggt
     3781 ggctgacgat ctccgggaag ccgtcgccgt cgaggtcgcc gatcgccggc gggctggcgg
     3841 cgacgacgcg atttagtgga tcgtcgatcg tctccagctg cgagcaatcg gtgccgttca
     3901 agatcctcac gacaccataa tagtcggggt tctcgcccca gccggccggg ccgccgccat
     3961 cggtgtagtt gtaggcgacg acgatgatct ccgccgcgac gcccgagtca ttgggcaggt
     4021 cgaagatcag cggggtcgtc agaacattct tgtgctcggg gtaggggtcg cccggcggtg
     4081 gcgcggtcca ctcgcactgc accgagg
//
LOCUS       DASWNJ010001933         4438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_493268, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001933 DASWNJ010000000
VERSION     DASWNJ010001933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493268"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(270..1082)
                     /locus_tag="VGB85_32250"
     CDS             complement(270..1082)
                     /locus_tag="VGB85_32250"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0138817.1"
                     /translation="MTLWPRMHLFEFHDRSWCPALLRQAVTDCVTTAWSFTGLWRNTV
                     PHLVDLLRRAGTDRIIDLCSGSGGPLPLAISAVAQEVPGVTATLTDLYPKPGLAEDLL
                     RRSGGRLTYHRAPVDAMAVPAELRGVRTIFGAFHHFAPKAAVQLLRGASAAGQPIAIF
                     EFQRRDFLRNCVPPTGLVGLSPWIALLLAPHRWWRAPLTILPVIPAVYAWDSVVTILR
                     TYTPEELAALAREASLPGYRWEVRDARGSGRARITCLAGFPDERGGVEVLRF"
     gene            1223..>4438
                     /locus_tag="VGB85_32255"
     CDS             1223..>4438
                     /locus_tag="VGB85_32255"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEY0138818.1"
                     /translation="MQRSLHIPAFFADDPALSALGIRASSRLWSAATGEPGSVDSARW
                     ISAWIAHVLQIDVAGIDPQARPFDLGLASIHGVQLAAALAVKLGRPVSPTLLWEHATI
                     AELAAHLDGAPPLVPASPLPHAGVRGPVAIVGMAGRFPKARDLDAFWELLADGVDATG
                     RVPADRWPADRWHDPDPHSPGKSVTDRGAFLDDVRGFEPLFFGISPREADELDPQQRL
                     ALELVWEALEDAGVPPRGLRGSRTGVYLGAMWHDWADLTHADLAGMSAHRATGTSNNL
                     IANRVSYVLGLRGPSLVLDTACSSALVAIHLACQALADGDAELAIAGAVNLMLSPAAS
                     VFCSKFGGLAPDGRCKTFDAAADGFARGEGGGVVVLKPLARALADGDRIHAIVRGSAV
                     NNDGASQGLTAPNPRAQEDVLRVALARAGLAGSQVHYVEAHGTGTLLGDPIEAGALGA
                     VFAVDRPDDRPLYIGSVKTNIGHLEGAAGIAGVIKTALAMRHRAIPPSLHLHEPNPHI
                     PFAALRLRVPTTLAAWPAPLDAPALAGVSAFGWGGTNAHVVLEAAPPAPRWIGVAAPD
                     EASLAVHLRALASTDLAQISAMNGAGPVRAAFVARSTHELREQLAVQRSACDGLVRGS
                     TTPRLVFVCSPLGSQWPGMARQLLAEPVFHAAILRCEQAFSPLLGRSIVDDLRGAWSG
                     IDDVVRVQPLLFSLQVGLAALLASWGVVPAAIVGHSAGEIAAAHIAGALTLEQAAGVV
                     HHYARVQQASAGAGAMAVVALPADALGEWLAPHAGQVVIAAHNSASSTVISGAAAAVA
                     AIVTAIQATGVACSPIHTDVAGHSPLVAAGLGDLRDSLAALTPAATERLHSSVTGGPC
                     DVPVDGAYWARNLGGVVRFHDAIGDLFARGHDTFVELGPHPVISHALQQIAGERGVPA
                     RILPTLRRGSDERMSLLTTRAALFVAGATGPEPAAPAHLLVLSARSEAALERTRDTIA
                     TWLATRPIDLHDLCFTAALRRSHLEHRLAVVGASREALATALQRPTRGRAGHPRIVFV
                     FPGQGSQWFGMGRVLLATRPVFRAAFDACAAALAPHLERSPL"
BASE COUNT          583 a         1752 c         1491 g          612 t
ORIGIN      
        1 cgccttgagc tggaggacct gccacatcgg cagcgacgcg cgcagcaccg cctcgggcac
       61 attactattt ttattaaagg cctcgaagcg cgacagcgcg accagcagcg cctcaccggt
      121 cgcgctggtc atggtgaccc cgcgtcgcgc acgacgatct cgaaccggcc gaccacgtgc
      181 tcacgatcgg ccgcgcgcac gaccctgtca acttcgttta atgcccccat gccgtgcgca
      241 cggtaaacca cgaagatcca cgttcggcgt cagaagcgca gaacctccac accgccacgc
      301 tcgtcgggga agccggccag gcaggtgatg cgcgcgcggc cgctgccgcg ggcgtcgcgg
      361 acctcccagc gatagccggg cagcgacgcc tcgcgcgcga gcgcggcgag ctcctcgggc
      421 gtgtatgtgc ggaggatcgt gacgacgctg tcccatgcgt acaccgccgg gatgacgggc
      481 aggatcgtca gcggcgcgcg ccaccagcga tgtggcgcga gcagcagcgc gatccatggc
      541 gagagcccga cgagcccggt cggcggcacg cagttgcgca ggaagtcgcg gcgctgaaac
      601 tcgaagatcg cgatcggctg gcccgccgcg ctcgcgcctc gcagcagctg caccgcggcc
      661 ttcggcgcga aatggtgaaa cgccccgaag atcgtccgga ccccgcgcag ctcggcgggc
      721 accgccatcg cgtcgaccgg cgcgcggtga tacgtcagcc gtccgcccga tcgccgcagc
      781 agatcctcgg cgagccccgg cttgggatag aggtcggtca gcgtcgcggt cacgccgggg
      841 acctcctgcg cgaccgccga gatcgccagc ggcagcgggc cgccggaccc cgagcagagg
      901 tcgatgatcc gatcggtgcc ggcgcgacgc aatagatcga cgaggtgcgg caccgtgttg
      961 cgccacagcc ccgtgaagct ccacgccgtc gtcacgcagt ccgtgacggc ctggcgcagc
     1021 aacgcggggc accacgatcg atcatggaac tcgaacaggt gcattcgcgg ccacagcgtc
     1081 atcgcgtctc agatatcccg ccacctcacc gctcgcaagt catgatctcg acgcgcgacc
     1141 tcagaccaca tgcatagatt cacaccagag gcgcgcgagt ccgcgatatt gctgatgagg
     1201 tgttgcgacc ccgtgcgcga tgatgcagag gtcgctgcat attccggcgt ttttcgccga
     1261 cgacccggca ctttcggcgc ttggcatccg agcgtcatct cgactatggt ccgccgccac
     1321 aggggagcct ggatccgtgg atagtgctcg ttggataagc gcatggatcg cccatgtgtt
     1381 gcagatcgat gtcgcaggca tcgatcctca ggcgcggccg ttcgacctcg ggctcgcgtc
     1441 gatccacggc gtgcagctcg ctgcggcgct cgccgtgaag ctcggccggc cggtgtcgcc
     1501 gacgctgctg tgggagcacg cgacgatcgc cgagctcgcc gcgcacctcg acggcgcgcc
     1561 acccctcgta cccgcgtcac cgctgccaca cgccggcgtg cgcggcccgg tggcgatcgt
     1621 cggcatggcc ggccgcttcc cgaaggcgcg cgacctcgac gccttctggg agctgctcgc
     1681 cgacggcgtc gacgcgaccg gccgcgtccc cgccgaccgc tggccggccg atcgctggca
     1741 cgaccccgac ccgcacagcc ccggcaagtc ggtcaccgac cgcggcgcct tcctcgacga
     1801 tgtccgcggc ttcgagccgc tgttcttcgg catctcgccg cgcgaggccg acgagctcga
     1861 cccgcagcag cggctcgcgc tcgagctggt gtgggaggcg ctcgaggacg ccggggtccc
     1921 gccacgcggg ctccggggca gccgcaccgg cgtgtacctc ggcgcgatgt ggcacgactg
     1981 ggccgacctc acccacgccg acctcgcggg catgagcgcg caccgggcca cgggcacctc
     2041 caacaatctg atcgccaacc gcgtgtcgta cgtgctcggc ctgcgcggcc cgagcctcgt
     2101 gctcgacacc gcctgctcgt ccgcgctggt ggcgatccac ctcgcgtgtc aggccctcgc
     2161 cgacggcgac gccgagctgg cgatcgccgg cgcggtcaac ctgatgctct cgcctgccgc
     2221 gagcgtgttc tgctcgaagt tcggcggcct cgcgcccgac ggccgctgca agaccttcga
     2281 cgccgcggcc gacggcttcg cgcgcggcga gggcggcggc gtcgtcgtgc tcaagccgct
     2341 cgcgcgggcc ctcgccgacg gtgaccgcat ccacgcgatc gtccgcggca gcgccgtcaa
     2401 caacgacggc gcgagtcagg gcctcaccgc cccgaacccg cgcgcccagg aggacgtgct
     2461 gcgcgtcgcg ctggcgcggg ccggcctcgc tggctcgcag gtccactacg tcgaggccca
     2521 cgggaccggc acactgctcg gcgacccgat cgaggccggc gcgctcggtg ccgtgttcgc
     2581 cgtcgatcgc cccgacgatc gcccgctgta tatcggctcc gtcaagacca acatcggcca
     2641 cctcgagggc gccgccggca tcgccggcgt gatcaagacg gcgctggcga tgcgccaccg
     2701 cgccataccg ccgtcgttgc atcttcacga gcccaacccg cacatcccct tcgccgcgct
     2761 gcggctgcgc gtcccgacca cgctcgcggc gtggcccgct ccgctcgacg cccccgcgct
     2821 cgcgggtgtg agcgccttcg gctggggcgg gaccaacgcg cacgtcgtgc tcgaggcggc
     2881 cccgcccgcg ccgcgctgga tcggcgtcgc ggcccccgac gaggcctcgc tcgccgtgca
     2941 cttgcgcgcg ctcgcatcga cggacctcgc acagatctcg gcgatgaacg gtgcgggccc
     3001 ggtgcgcgcg gcgttcgtcg cccgctcgac ccacgagctg cgcgagcagc tcgccgtgca
     3061 gcgcagcgcc tgcgacggcc tcgtgcgtgg ctcgaccacg ccgcggctcg tattcgtgtg
     3121 ctcgccgctc ggctcacagt ggcccggcat ggcccgccag ctgctcgccg agccggtgtt
     3181 ccacgcggcg atcctccgct gcgagcaggc cttcagtccg ctgctcgggc gatcgatcgt
     3241 cgacgacctg cgcggcgcct ggtccggcat cgacgacgtc gtccgcgtgc agccgctgct
     3301 gttctcgctg caggtcggcc tcgccgcgct gctcgcctcc tggggcgtcg tgccggcggc
     3361 gatcgtcggc cacagcgcgg gggagatcgc cgcggcgcac atcgccggcg ccttgaccct
     3421 cgagcaggcc gcgggcgtcg tccatcacta cgcccgagta caacaggcca gcgccggtgc
     3481 cggtgcgatg gccgtcgtcg ccttgccggc cgacgcgctc ggcgagtggc tcgcgccgca
     3541 cgccggccag gtcgtgatcg ccgcgcacaa cagcgccagc agcaccgtga tctccggcgc
     3601 cgcggcggcc gtcgccgcga tcgtcaccgc gatccaggcc accggcgtcg cctgctcgcc
     3661 gatccacacc gacgtcgccg gccacagccc gctcgtcgcc gccggcctcg gcgacctgcg
     3721 cgacagcctc gccgcgctga cccccgcggc gaccgagcgc ctgcactcga gcgtcaccgg
     3781 cggaccctgc gacgtccctg tcgacggcgc ctactgggcc cgcaacctcg gcggcgtcgt
     3841 gcgcttccac gacgcgatcg gcgacctgtt cgcgcgcggc cacgacacct tcgtcgagct
     3901 cggcccgcac ccggtcatca gccacgcctt gcagcagatc gccggcgagc gcggtgtgcc
     3961 ggcgcggatc ctgccgacgc tgcggcgcgg aagcgacgag cgcatgtccc tgctgacgac
     4021 ccgcgccgcc ctgttcgtcg ccggcgcgac cggccccgag ccggccgccc ccgcgcacct
     4081 gctcgtgctc tccgcgcgca gcgaggccgc cctcgagcgc acgcgcgaca cgatcgccac
     4141 ctggctcgcg acccgcccga tcgatcttca cgacctctgc ttcaccgccg ccctgcgacg
     4201 ctcgcacctc gagcaccgcc tcgcggtcgt cggcgcctcg cgcgaggccc tcgcgaccgc
     4261 gctgcaacgg ccgacccgcg gacgcgccgg ccacccgcgg atcgtgttcg tgttcccggg
     4321 gcagggctcg cagtggttcg gcatgggccg ggtcctgctc gccacccggc cggtgtttcg
     4381 cgccgccttc gacgcctgcg ccgcagcgct cgcgccccac ctcgagcgct cgcccctc
//
LOCUS       DASWNJ010001934         3480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_173976, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001934 DASWNJ010000000
VERSION     DASWNJ010001934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173976"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="VGB85_32260"
     CDS             complement(<1..1034)
                     /locus_tag="VGB85_32260"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEY0138819.1"
                     /translation="MRLGTLLADLDPAYHGCSPDTEIAGIVVDSRRAAPGLAFLAVAG
                     LTNDGHDFLAQAAAAGALLLLQRGRVPAPTHPHVWLRDTTATLPTLAARSFGEPANHL
                     RLAGVTGTNGKTTVTYLVAAMLTAAGRKHVRLGTAGNWLVDHDAPAAFTTPFPLELQG
                     LLAEAVARGATDLVMEVSSHALAQGRVQPLRYHAVGMTSFSQDHLDFHGTMADYLAAK
                     RLLASDYLRPDGIAVAAVDAQPAAHEFLAAARELGARTWRASRGADPDAELCVLRDMS
                     SGTGFDVQLRTPAGAARLRSPLIGGYNLDNALVALGLALGLGVPLEQALAGLETSRGA
                     PGRLERVTVAG"
     gene            complement(1125..1847)
                     /locus_tag="VGB85_32265"
     CDS             complement(1125..1847)
                     /locus_tag="VGB85_32265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138820.1"
                     /translation="MVGIRRAISLLLLSLFFWNFVLTALLGPDELFACFTGLSIVYGL
                     AFVGIAAEWFWARWFAIGLGNFGALSLLTLLQTGLEPTIAVFGASHLIISLFLAGEGM
                     ASRYEHSESTAERWNFQEESLVLLRRAVKSAGMSLPLLILYTLAPKPELLHLVALVAG
                     VTGVAGLLRGRTWGLAGMGLAGGIALADGFGLFGPPTVSYLLLTPSGSVVLWGSAFAL
                     LAGTMIAAPLAFARPIARFLRG"
     gene            2091..3239
                     /locus_tag="VGB85_32270"
     CDS             2091..3239
                     /locus_tag="VGB85_32270"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEY0138821.1"
                     /translation="MKNVSQKLRSLLRAPLSAVSRRGRLAVALTASLASAGCALTVTR
                     LNSAQKKPNNVWVFFTVEKGKEPVAGLAAEDFDIYEDGDLVSVYESKQVIQNPEVAAV
                     MYTMLLLDVSGSITESGSSDTLVDAATLFTDRVGKTQKVGVYAFDGEKEIKSVVPFTE
                     SEGAAVGGLEGLRTYKAKDPSTNLHGAVIEGLRELKKQLDKEKKPLKFGTLVVFTDGT
                     DRAARFSRDDMKTEIIKPQYEDYELYAIGVGVEIKKAGLNDIGQDGTELVKEQAEVKA
                     AFEKVAERIEKHTKRFYLLSYCTPARKGTHEVRIVANSKNDPKGKGDLEWDFNADGFG
                     PPPDCDPNTPPSFPLKTNGQPVEGGDDNGGGNGGGGVKVKASASASAK"
BASE COUNT          595 a         1106 c         1349 g          430 t
ORIGIN      
        1 cccgcgacgg tgacgcgctc gagccggccc ggggcgccgc ggctggtctc gaggccggcg
       61 agcgcctgct cgagagggac gccgaggccg agcgcgaggc cgagggcgac gagcgcgttg
      121 tcgaggttgt agccgccgat caacggcgag cgcaggcggg ccgcgccggc gggcgtgcgc
      181 agctgcacgt cgaagcctgt acccgaggac atgtccctta gaacacacag ctcggcgtcg
      241 gggtcggcgc cgcgagaggc tcgccaggtg cgggcgccga gctcgcgcgc ggcggcgagg
      301 aactcgtgcg cggccggctg ggcgtcgact gcggcgacgg cgataccgtc agggcgcagg
      361 tagtcgctgg cgagcaggcg cttggcggcg aggtagtcgg ccatcgtgcc gtggaagtcg
      421 aggtggtcct gcgagaagct ggtcatgccg acggcgtggt agcgcagcgg ttgcacgcgg
      481 ccctgggcga gcgcgtgcga ggagacctcc atgacgaggt cggtcgcgcc gcgggccacg
      541 gcctcggcga gcaggccttg cagctcgagc gggaaggggg tggtgaaggc ggcgggggcg
      601 tcgtggtcga cgagccagtt gcctgccgtg ccgaggcgga cgtgcttgcg tccggcggcg
      661 gtcagcatgg cggcgacgag gtaggtgacg gtcgtcttgc cgttggtgcc ggtgacgccg
      721 gcgaggcgga ggtggtttgc gggctcgccg aagctgcggg cggcgagcgt cggcagtgtg
      781 gcggtcgtgt cgcgcagcca cacgtgcggg tgcgtcggcg cgggtacgcg gccgcgttgc
      841 agcagcaaca gcgcgccggc ggccgcggcc tgcgcgagga agtcatggcc gtcgttcgtc
      901 agtccggcga cggcgaggaa ggcgaggccg ggcgcggcgc gccgcgagtc gacgacgatg
      961 ccggcgatct cggtgtcggg cgagcagccg tgatacgcgg ggtcgaggtc ggcgagcagg
     1021 gtcccgaggc gcatgcgcgg acgatagcgg gctcgctgcg cgccggacca aaaaaagcgg
     1081 ccggctcgtg ggagccggcc gccgaagtgt cgcgcccgaa gttatcagcc gcgcaggaag
     1141 cgggcgatcg ggcgggcgaa ggcgaggggg gcggcgatca tcgtgccggc gagcagggcg
     1201 aaggccgagc cccacaggac caccgagccg ctcggggtga gcaggaggta gctgacggtc
     1261 ggcggcccga acaggccgaa gccgtccgcg agggcgatgc cgccggcgag gcccatgccg
     1321 gcgaggcccc aggtgcggcc gcgcagcaaa ccggcgacgc cggtgacgcc ggcgacgagc
     1381 gcgacgaggt ggagcagctc cggcttgggg gcgagggtgt agaggatcag cagcggcagc
     1441 gacatgccgg cggacttgac ggcgcggcgc agcaggacga gcgactcctc ctggaagttc
     1501 cagcgctcgg cggtgctctc ggagtgctcg tagcgggagg ccatgccctc accggcgagg
     1561 aacagcgaga tgatgaggtg cgaggcgccg aacacggcga tcgtcggctc gaggccggtc
     1621 tggaggaggg tcagcaggga cagggcgccg aagttgccga ggccgatggc gaaccaccgg
     1681 gcccagaacc actcggcggc gatgccgacg aacgcgagcc cgtagacgat cgacagaccg
     1741 gtgaagcagg cgaacagctc gtccgggccg agcagggccg tgaggacgaa gttccagaag
     1801 aacagactga ggagaaggag ggagatcgcg cgtcggatgc ctaccatgta gcctcggaac
     1861 gacaagccta cgcccggggg cggggcggcg caacgtcgtc acgcggctac tgcgacgaga
     1921 tcgacgcacg accccgctcg cgctgagacc ggcccgatcg gatcggttcc aggtcccctg
     1981 cgatcgccgg tgaattctgg ccaggcgccg gcctcggcgg gtcttcgcgg cggatccgcc
     2041 cgtcggaacg attgacaagc gggggcctgg ctggagtaat cctgcccgtg atgaagaacg
     2101 tgagccaaaa gctgaggtcc ctgcttcgtg cgccgctgtc ggcggtctcc cggcgcgggc
     2161 gcctcgcagt ggcgctgacc gcctcgctgg cgtcggcggg ctgcgcactg accgtgacgc
     2221 gcctcaactc cgcgcagaag aagcccaaca acgtgtgggt gttcttcacc gtcgagaagg
     2281 gcaaggagcc ggtcgcgggc ctcgccgccg aggacttcga catctacgag gacggcgacc
     2341 tggtgtccgt ctacgagagc aagcaggtga tccagaaccc cgaggtcgcg gcggtgatgt
     2401 acacgatgct gctgctcgac gtgtcgggca gcatcaccga gtcgggatcg tccgacacgc
     2461 tggtcgacgc ggcgacgctg ttcaccgacc gcgtcggcaa gacccagaag gtcggcgtgt
     2521 acgcgttcga cggcgagaag gagatcaagt cggtcgtgcc gttcaccgag tccgagggcg
     2581 cggcggtcgg cggcctcgag ggtctgcgca cctacaaggc caaggacccg tcgaccaacc
     2641 tgcacggcgc ggtgatcgag ggcctgcgcg agctcaagaa gcagctcgac aaggagaaga
     2701 agccgctcaa gttcggcacg ctggtggtct tcaccgacgg caccgaccgc gcggcccgct
     2761 tctcgcgcga cgacatgaag acggagatca tcaagccgca gtacgaggac tacgagctgt
     2821 acgcgatcgg cgtcggcgtc gagatcaaga aggccggcct caacgacatc ggccaggacg
     2881 gcaccgagct ggtcaaagag caggccgagg tcaaggcggc gttcgagaag gtcgccgagc
     2941 ggatcgagaa gcacaccaag cgcttctacc tgctcagcta ctgcaccccg gcgcgcaagg
     3001 gcacccacga ggtccgcatc gtcgccaaca gcaagaacga ccccaagggc aagggcgacc
     3061 tcgagtggga cttcaacgcc gacggcttcg ggccgccccc ggactgcgac ccgaacacgc
     3121 cgccgtcctt cccgctcaag accaacggcc agcccgtcga gggcggtgac gacaacggcg
     3181 gcggcaatgg tggcggcggc gtcaaggtga aggccagcgc gagcgcgagc gcgaagtagc
     3241 cgggcccgcg taacgccgtc atggggcgcc ggaaagcgtc cctggccttc tgcgcggatg
     3301 gcttgcagcc acctgcgcag aaagttcacg tttgcatcta cccgcggccc ggcgggtagg
     3361 ctaaaaacga gcatggtcgc tttcgcgccc ctgctcgccc tgttcaccct cgttgcaccg
     3421 gctcggccgc cgttgtcgcc cacggccgac aacgaggcca tctacgacga gatcgtcgcc
//
LOCUS       DASWNJ010001935         3844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_670057, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001935 DASWNJ010000000
VERSION     DASWNJ010001935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670057"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            400..1413
                     /locus_tag="VGB85_32275"
     CDS             400..1413
                     /locus_tag="VGB85_32275"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY0138822.1"
                     /translation="MLSIHWFAGIAVASTLSLMPDVAAAKPKPAPVAEPEVLVPLDPN
                     QGQLPESITTDEDGNIYLSIGNTIGIIDPDGNYSTFATLPIPTGGFVTGLKFGDDGYL
                     YAGTGAFSTDPSGAFVYRVSPDGEVIEQCAALPANGFPNDLAFDNKGNLYVTDPFLGQ
                     IYKIDGCGGDVSVFLDDPLLDPNTGDPFLGISPFGVDGIAFDKNYKNLYVGNLDYGRI
                     VKIELEKNGTAGDVDVFYENVDLIGGVDGIAFDKQGTLYLAVHGQERIVAIDKKGKAT
                     IVAEGGELNQPSSLVFGATKNDKNTLYISNFSILSATGFRPGPPTPGLLTLDVKHGGQ
                     SLP"
     gene            complement(1470..2531)
                     /locus_tag="VGB85_32280"
     CDS             complement(1470..2531)
                     /locus_tag="VGB85_32280"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138823.1"
                     /translation="MAPTRAAPHLSRRTYLLGLMALAACRGRERGSPPASEPVASAEP
                     PTTQAGPTPTAAVAAAVPAPAVASGGPLPVFYDPHQHTEANDSFSPSAGKPAKVVARW
                     QRSKVPVRLMPVEPVTAEQLALAHSREYVDGVLAGRLPNGFGNTLKEVAATLPWTTGS
                     LVSAAIHVAQNGGVAVSPTSGFHHAHYSSGGGFCTFNGLMVAVRILQSRGLARKIGIL
                     DLDMHYGNGTADILERLKVADVHHYTFGAHYGTPEQGEEFIKELPQILAGFAGCELVL
                     YQAGADSFVDDPLGGVLTMPQLRMRDRLVFRHFAAAKTPLVWDLAGGYARDAAGTIEP
                     VLSIHDATLEECHAAYAGA"
     gene            3113..>3844
                     /locus_tag="VGB85_32285"
     CDS             3113..>3844
                     /locus_tag="VGB85_32285"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY0138824.1"
                     /translation="MTKLSCLRVFAMMLASGVAISACDDEFSAEELSDPASLLGDDLW
                     WDEGPPAGEQSIEQVGEITAELDPTLAPAFQLPFPCNQVWAGQTRTDHSPQNSVDFNR
                     TNDVDDPVVASAAGKVSRVANEGATSYGRWVEIDHGSGYRSRYAHLNSQTVSVGQSVT
                     RGQKIGTVGSTGGSSGPHLHYEVRLNGTAIKPVFNGSTALFFGTKNYTSNNCGGGGGG
                     ASFPGTVNTNGIALTIRSTPSTSGTA"
BASE COUNT          643 a         1357 c         1269 g          575 t
ORIGIN      
        1 ccacgtgggg tcgagagacg gcaatcgcga gccgcacacg gccgacgtcg tcgcggctca
       61 ccgcacgcag gccgagcgcg acggcgggga gatacccccg gcgtcgcgct caggaccgcg
      121 accgatgcca cgatagcgcg ccgatcgacg tcattgcata tgccgcgatg taggcaggca
      181 gggccgtcgc gacccggcgt atgtgcatgg cgtcgactgc tgacaatgcg ggcctcgtgg
      241 gcgcaaaacc cagccccacg ggcacgagga ggctaatttt gcgaagaaag ttgattgaaa
      301 cctttcaact acggtgcata tcataaccga gtaatcgttc gtcgtggtcg cccacggcgg
      361 acgaatagtg atagttcgta cctgtacagg agcaagcgta tgttgagcat tcactggttc
      421 gccgggatcg cggtcgcctc caccctgagc ctcatgcccg atgtcgctgc ggccaagccg
      481 aagcccgcgc ccgtggccga gcccgaggtc ctcgtgcccc tcgatccgaa ccagggccag
      541 ctccccgagt cgatcacgac cgacgaggac ggcaacatct acctctcgat cggcaacacg
      601 atcggcatca tcgaccccga cggcaactac agcacctttg cgaccctgcc gatcccgacc
      661 ggcggcttcg tgaccggcct gaagttcggc gacgatggct acctgtacgc cggcactggc
      721 gccttctcca ccgacccctc gggcgccttc gtctaccgcg tctcgcccga cggcgaggtg
      781 atcgagcagt gcgcggcgct gcccgccaac ggcttcccca acgacctcgc gttcgacaac
      841 aagggcaacc tctacgtcac cgaccccttc ctcggccaga tctacaagat cgacgggtgt
      901 ggcggcgatg tctcggtgtt cctcgacgac ccgctgctcg acccgaacac cggcgacccc
      961 ttcctcggca tctcgccgtt cggcgtcgac ggtatcgcct tcgacaagaa ctacaagaac
     1021 ctgtacgtcg gcaacctcga ctacggccgc atcgtcaaga tcgagctcga gaagaacggc
     1081 accgccggcg atgtcgacgt gttctacgag aacgtcgacc tgatcggcgg cgtcgacggc
     1141 atcgccttcg acaagcaggg gaccctgtac ctcgcggtcc acggccagga gcgcatcgtc
     1201 gcgatcgaca agaagggcaa ggcgacgatc gtcgccgagg gcggcgagct caaccagccc
     1261 tcgagcctgg tattcggcgc gaccaagaac gacaagaaca ccctgtacat ctcgaacttc
     1321 tcgatcctgt cggcgaccgg ctttcgcccg ggtccgccga ccccgggcct gctgaccctc
     1381 gacgtcaagc acggcggcca gagcctgccc tgagcctccg gcgcgcgcgc gccgaggccc
     1441 tgtcccttcg gacagggcct ctcgccgttt cacgcccccg cgtaggcggc gtgacactcc
     1501 tcgagcgtgg cgtcgtggat cgacagcacc ggctcgatcg tgccggcggc gtcgcgcgcg
     1561 tagccgccgg cgaggtccca gaccagcggg gtcttcgcgg ccgcgaagtg gcggaacacc
     1621 aggcggtcgc gcatgcgcag ctgtggcatc gtcagcacgc cgccgagcgg gtcgtcgacg
     1681 aagctgtcgg cgccggcctg atacagcacc agctcgcagc cggcgaagcc cgcgaggatc
     1741 tgcggcagct ctttgataaa ttcctcgccc tgctcgggcg tgccgtagtg cgcgccgaag
     1801 gtgtagtggt ggacgtcggc gaccttgagc cgctcgagga tgtcggcggt gccgttgcca
     1861 taatgcatgt cgaggtcgag gatcccgatc ttgcgcgcga gcccccggct ctgaaggatc
     1921 cgcaccgcga ccatcaggcc gttgaaggtg cagaagccgc ccccgctgct gtagtgcgcg
     1981 tggtgaaagc cggaggtcgg cgagaccgcg acgccgccgt tttgcgcgac gtggatcgcc
     2041 gccgacacca gcgagccggt cgtccacggc aaggtcgccg ccacctcctt caaggtgttg
     2101 ccgaagccgt tgggcaggcg tcccgcgagc acgccgtcga cgtactcgcg gctgtgcgcc
     2161 agcgcgagct gctcggccgt caccggctcg accggcatca ggcgcaccgg caccttcgag
     2221 cgttgccagc gcgcgacgac cttcgccggc ttgcccgcgc tcggactgaa ggagtcgttg
     2281 gcctcggtgt gctggtgcgg gtcgtagaac accggcagcg gaccgccgct cgccacagcg
     2341 ggcgccggta ccgccgctgc gacggcggcc gtcggcgtcg gtccggcctg cgtcgtcggc
     2401 ggctccgcgc tggcgaccgg ctcgctcgcg ggcggcgagc cgcgctcacg cccgcggcag
     2461 gccgcgaggg ccatcagtcc gagcaagtat gtcctccggg acaggtgcgg ggccgctcgc
     2521 gtcggtgcca tgtcgcggaa catggcacgg cacgccgcgc gggccagccg gaggcgccgt
     2581 cgcgggtgat gcagggcgcg gatgcccggc gctcgccgcg aacgggccag gccgtcgcgg
     2641 ggcccgcctc atggcccacg aaggtgggca tgcgagcggc tggacggcgc gacgacgagc
     2701 gacatcggcg tgtacgacac cgtgtcctga cagtgacacc cgggcacatt cgcgggctgt
     2761 ccgaggggtg tcgagacccc gtcgagcgcc tgtgatcctg agtaacgacg tccggacttt
     2821 cgatcacgaa atctcaggag ccgccgagtg aggtcacgaa aaaaccggga cgagtggccc
     2881 gcgaaatgcc ggctgcgggc cactcgcggg ccagttgtag gccacaggca gccagtggac
     2941 gaagttcatg acccttgcac tcggcgtgcg tccgggtgca tagcgatgat gcagtgtgga
     3001 tcgccgttct cggcgctcgc agccgcggcc ggacaggtcg cagcttcgcg cgcgtcgagg
     3061 tgcggtgcga agtattccct cgtgtttttt gcgcctccaa aggactccga tgatgaccaa
     3121 gctttcttgc ctccgtgtgt tcgccatgat gctcgccagc ggcgttgcca tcagcgcctg
     3181 tgacgacgag ttctccgcgg aagagctgag cgaccccgcc agtctgctcg gcgatgacct
     3241 gtggtgggac gagggtcccc cggccggcga gcagtcgatc gagcaggtcg gtgagatcac
     3301 cgcggagctc gacccgacgc tcgcccctgc cttccagctg ccgttcccgt gcaatcaagt
     3361 ttgggccggc cagacccgca ccgaccacag cccgcagaac tcggtcgact tcaaccgcac
     3421 caacgacgtc gacgacccgg tcgtcgcgtc ggccgccggc aaggtcagcc gcgtcgccaa
     3481 cgagggcgcg accagctacg gccgctgggt cgagatcgat cacggcagcg gctaccgcag
     3541 ccgctacgcg cacctcaact cgcagaccgt ctcggtcggc cagagcgtca cccggggcca
     3601 gaagatcggc accgtcggca gcaccggcgg ctcgagcggc ccgcacctcc actatgaggt
     3661 gcgcctcaac ggcaccgcga tcaagccggt gttcaacggc agcaccgccc tgttcttcgg
     3721 caccaagaac tacaccagca acaactgcgg cggcggtggc ggcggcgcca gcttccccgg
     3781 caccgtgaac accaacggca tcgcgctgac gatccgctcg accccgagca ccagcggcac
     3841 cgcg
//
LOCUS       DASWNJ010001936         1590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_69337, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001936 DASWNJ010000000
VERSION     DASWNJ010001936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69337"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VGB85_32290"
     CDS             complement(<1..271)
                     /locus_tag="VGB85_32290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138825.1"
                     /translation="MAIDMTAADLEGPCVAEIVAIYAGLVFDVRHCARGCYTIGDGPL
                     VSFPLLSQALPDPSCFPLVRREGGATWLHFTVDMRGELVEAAGRSS"
     gene            complement(335..1210)
                     /locus_tag="VGB85_32295"
     CDS             complement(335..1210)
                     /locus_tag="VGB85_32295"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY0138826.1"
                     /translation="MNAALATKLATVVTPTHRAIVHRTRGRKHGPITRLMSPGDVGER
                     VKPFVFLDYFETAGFSGGGFAAHPHSGIATHTTLIRGTFDYGDSTGKEGVLREGNVEW
                     MQAGGGVWHWGDPRPGEPVRGYQLWVALPAEIEHAPAESHYVDTARIETDGTVRVLLG
                     RYGALGSPIPYAEPITYLHVTLADGQRWTFQPEAGHDVAWLAVHRGALQTADRTRLAD
                     EMVVFEDGAGAIDLEAIGPTELVIGAARRHPHPLVCGYYSVHTSPAALVRGEQGIDEV
                     ARTPRVREAVRRTSR"
     gene            1300..>1590
                     /locus_tag="VGB85_32300"
     CDS             1300..>1590
                     /locus_tag="VGB85_32300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138827.1"
                     /translation="MHHFAMKLALPLAVTLLALACAPRSTPPPPPDVRGDVLTATEVE
                     ALSAAHHAAGAAFGRSDFVAVAAAFADGLKINPGDDTLVYQQAAALALAGRHD"
BASE COUNT          211 a          622 c          542 g          215 t
ORIGIN      
        1 gcgagctgcg gccggcggcc tcgacgagct cgccgcgcat gtcgaccgtg aagtgcagcc
       61 aggtcgcgcc gccctcgcgt cgcaccagcg ggaagcagct cgggtctggc agcgcctgcg
      121 acagcagcgg gaacgacacc agcggcccgt cgccgatcgt atagcagccg cgcgcgcagt
      181 gtcgcacgtc gaacaccagg cccgcgtaga tcgccacgat ctcggcgaca cagggtccct
      241 cgaggtcggc ggcggtcata tcgatcgcca acgcggccgc cgacgcgaag ttctacccgt
      301 cctgtccggc ggtcgcacga acctccagcc gggatcagcg cgacgtcctg cgcacggcct
      361 cgcgcacccg cggcgtccgg gcgacctcgt cgatcccctg ctccccgcgg accagcgcgg
      421 ccgggctggt gtgcaccgag taatagccgc acacgagcgg atgcgggtgc ctgcgggccg
      481 cgccgatcac gagctcggtc ggcccgatcg cctcgagatc gatcgcaccg gcgccgtcct
      541 cgaacacgac catctcatcc gcgagccgcg tgcggtccgc ggtctgcagc gccccgcggt
      601 ggaccgcgag ccacgcgacg tcgtgcccgg cctcgggctg gaacgtccac cgttggccgt
      661 ccgccagcgt cacgtggaga tacgtgatcg gctcggcgta cgggatcggg ctgccgagcg
      721 cgccgtacct gccgagcagc acccgcaccg tcccgtccgt ctcgatccgc gcggtgtcga
      781 catagtggct ctcggcgggc gcgtgctcga tctccgccgg cagcgcgacc cacagctgat
      841 agccgcgcac cggctcaccg ggccgcgggt cgccccagtg ccacacgccg ccgccggcct
      901 gcatccactc gacgttgccc tcgcgcagca cgccctcctt gccggtcgag tcgccgtaat
      961 cgaaggtccc gcggatcagc gtcgtgtggg tcgcgatgcc cgagtggggg tgggccgcga
     1021 agccgccgcc cgaaaagccc gccgtctcga agtagtcgag gaacacgaac ggcttgaccc
     1081 gctcgccgac gtcccccggg ctcatcagcc gggtgatcgg gccgtgcttg cggccgcgcg
     1141 tgcgatgcac gatggcgcga tgcgtggggg tgacgacggt ggcgagcttc gtggcgagcg
     1201 ctgcgttcat gacgactcca ctgtgcgccg cccggcgccg gagacgagcc ctcgccgcgg
     1261 caacaggctg ttgcaccgct gcaccccccg cgtgttagcg tgcaccactt cgccatgaag
     1321 ctcgcgctgc ccctcgccgt cacgctcctc gccctcgcct gcgccccgcg gtcgaccccg
     1381 ccgccgccgc cggatgtccg gggcgacgtg ctcacggcga ccgaggtcga ggccctgtcg
     1441 gccgcccacc acgccgccgg cgcggccttc gggcgcagtg attttgtcgc ggtcgcggcc
     1501 gccttcgccg acggcctgaa gatcaacccc ggcgacgaca ccctcgtgta tcagcaggcc
     1561 gccgcgctcg cgctcgccgg ccggcacgac
//
LOCUS       DASWNJ010001937         1924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_345332, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001937 DASWNJ010000000
VERSION     DASWNJ010001937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345332"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..1817
                     /locus_tag="VGB85_32305"
     CDS             219..1817
                     /locus_tag="VGB85_32305"
                     /inference="COORDINATES: protein motif:HMM:NF025600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HEY0138828.1"
                     /translation="MTLLCPPACGDAGAGETGDSTPATTSGSTGDTSGTTGDAPTSTT
                     GDTTSITPTGTDTGDAGWTPSTCYDGWDELLDRYPDAGALQDCAGVPGGLAVQRSLLV
                     VDGITIDNNGEAMQPCVEARCDASYAYIASNDLPHYDYVQTNPNPLMEAPGIVRIALA
                     PALPAGDVTADPATIQNGCGDAYAQYLDDPGQATAAEPSGLCSAGQGGLLEYLRETLE
                     SGETATYAKILCLGGIGVIVNGVTANGPNEAQTPDPYGNPVFGMPEVAGDFDQTAFLD
                     LCGGHSGGSMHYHGLNEACFEQAADGTPASSYATASQGWDLAKMLTGACTEPSGIIGW
                     SLDGYPIQGPCVCTARAGDACTQVKRVRSAWVHDGLGGWGEDPGEGAALGVEGTICDD
                     DNICCPGGGAGCDFRCAPVIVDSDDPDGSAVEKRCALLDYAWCTHRYVDRSLHAGGDF
                     VYLDRCNGYEGPDGYAYHATASFPYIQGCYRGVPNLAPMGGMMMGDDDGGMEPPKCMQ
                     GQTMCCGDATCDGPETADNCPEDC"
BASE COUNT          292 a          678 c          707 g          247 t
ORIGIN      
        1 cgtacgtacg ctgtacgaca agatcaagcg ctacgggctc gaggacgagc tcggccgtgg
       61 gtgaggtcgg cagctctcgc cgggtcgccc gacgcggatc cggcgagagc tgcccgacgg
      121 gccgccgcgg ccggcacaaa ccaccgcggc ggggcccgac agcgcctggc acggcgttcg
      181 cagtgccggg agccatgcgc atccgtcggc tcctcctctt gacgctcctt tgtcctccgg
      241 cctgcggtga tgccggcgcc ggcgagaccg gcgacagcac gccggcgacg acctccggca
      301 gcaccggcga cacgtcgggt accaccgggg acgcgccgac cagcacgacc ggcgatacca
      361 cgagcatcac gccgacgggc acggacacgg gcgacgccgg ctggacgccc tcgacctgct
      421 acgacggctg ggacgagctg ctggaccgct accccgacgc gggcgcgttg caggactgtg
      481 cgggcgtgcc cggcgggctc gcggtgcagc gctcgctgct ggtggtcgac ggcatcacga
      541 tcgacaacaa cggcgaggcg atgcagccct gcgtcgaggc ccgctgcgac gcgagctacg
      601 cgtatatcgc cagcaacgac ctgccgcact acgattatgt gcagaccaac ccgaacccgc
      661 tgatggaggc gccggggatc gttcgcatcg cgctggcgcc ggcgctgccc gcgggcgacg
      721 tgacggccga tccggcgacg atccagaatg gttgcggcga cgcgtacgcg caatacctcg
      781 acgaccccgg gcaggcgacc gccgccgagc cctcggggct gtgcagcgcc gggcagggcg
      841 ggttgctcga gtatctgcgc gagactttgg agagcggcga gacggcgacc tacgccaaga
      901 tcctgtgcct cggcggcatc ggcgtgatcg tcaacggcgt caccgcgaac gggcccaacg
      961 aggcgcagac gccggacccc tacggcaacc cggtgttcgg catgcccgag gtcgcgggcg
     1021 acttcgacca gaccgcgttc ctcgacctgt gcggcggtca ctccggcggc agcatgcact
     1081 accacgggct caacgaggcg tgcttcgagc aggccgccga cggcacgccg gccagcagct
     1141 acgcgacggc ctcgcagggc tgggacctgg cgaagatgct gaccggcgcg tgcacggagc
     1201 cctcggggat catcggctgg agcctcgacg gttacccgat ccaggggccc tgtgtgtgca
     1261 cggcccgcgc gggcgatgcg tgcacgcagg tcaagcgcgt gcgctcggcg tgggtccacg
     1321 acggcctcgg cggctggggc gaggatccgg gcgagggcgc ggcgctcggc gtcgagggca
     1381 cgatatgcga cgacgacaat atctgctgcc ccggcggcgg ggccggctgc gactttcgtt
     1441 gcgcgccggt gatcgtcgac agcgacgacc ccgacggatc cgcggtcgag aagcgctgcg
     1501 cgctgctcga ctatgcgtgg tgtacgcacc gttatgtcga ccgctcgctg cacgcgggcg
     1561 gcgacttcgt gtacctcgac cgctgcaacg gctacgaggg ccccgacggc tacgcgtatc
     1621 acgcgaccgc gtcgttcccg tacatccagg gctgttatcg cggcgtcccc aacctcgcgc
     1681 cgatgggcgg gatgatgatg ggcgacgacg acggcggcat ggagccgccc aagtgcatgc
     1741 agggccagac gatgtgctgc ggcgacgcga cctgcgacgg gcccgagacg gccgacaact
     1801 gcccggagga ctgctgatgt tcgccgcgcg catgctgatg atcgcggcgc tcgccggcgg
     1861 ctgcggggca gggaacgacg acgccccgcg cccggtcggc ccggccgcgc cgctgcagct
     1921 cggc
//
LOCUS       DASWNJ010001938         4005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_105389, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001938 DASWNJ010000000
VERSION     DASWNJ010001938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105389"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2152
                     /locus_tag="VGB85_32310"
     CDS             <1..2152
                     /locus_tag="VGB85_32310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0138829.1"
                     /translation="VKQSIQEVVKTLFEAVVLVFLVMFLFLQNWRATLIPTIAVPVVL
                     LGTFAVLSALGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVMSEDGLPPKEATRRS
                     MDQISGALVGIALVLAAVFVPMAFFGGSVGMIYRQFSITLVSSMALSVVVAMTLTPAL
                     CATMLKPGHGPRTRGFFGLFNRGYDRSSGGYQRVIGVLVRRPFLAMVGFLAIVGALVL
                     LFVRMPTGFLPEEDQGALNVQVLLPPGSTMERTRAVLDRVSDHFLEQEGDAVESVLAI
                     SGFGFSGRGQNSGIAFIRLKNWEERGDARQRASAIAGRAMGAFSKIKEATIFAVVPPA
                     ISELGNATGFQFQLQDRGGLGHEALIASRDQLLGLANKQPELTRVRQGGLEDTPQYKI
                     DVDQEKAVALGLSLSDINATLQATWGGSYVNDFIDRGRTKRVYIQGDAPFRMQPDDID
                     RWYVRNKDGKMVPFSAFSTGHWTYGSPKLDRFNGFPTILIQGEAAPGSSSGAAMATME
                     RLAGELPAGIGFQWSGLSYEEVASGANAPMLYALSLLVVFLCLAALYESWSIPAAVML
                     VVPLGVAGAVSATLGFGLANDVYFQVGLLTTVGLSAKNAILIVEFAKELREQGKGRLE
                     SALEAARARLRPILMTSLAFVLGVLPLAIASGAGAGAQNAIGIAVIGGVLAGTVLGVL
                     FVPVFYVVIARRDPKREPRLDAPIARPPADPVHA"
     gene            2220..3575
                     /locus_tag="VGB85_32315"
     CDS             2220..3575
                     /locus_tag="VGB85_32315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate kinase"
                     /protein_id="HEY0138830.1"
                     /translation="MTTTTSLPAKVLHDLTTALSATQARLAERYPGEPGTRQPVHTVY
                     GGAHLFRHDTAKKLGELGLRVLRQYAPDAATFASAIGLESSHADTIYERVLEKMQREA
                     VEDFRIDFEDGYGNRSDAEEDGHAVQAGEEVARGMKEGTLPPFIGIRIKTLSGELCAR
                     ALRTLDVFVTTVVRNSGGHLPDNFVVTLPKITSPEHVTTLVATFEALESSLALLQGSL
                     KMEFMIETTQSILDVDGSIMLPRMLTAAQGRCVAAHFGVYDFTASNDITAQYQRMGHP
                     ACDFARHMMKMAYAGTGLWLSDGATNIMPVAPHRAEAGGTLTAEQTAENRKAVHAAWK
                     LHADDIFHSLESGYYQGWDLHPGQLPTRYAATYAFFLRGLASASERLSNFVSKAAQAT
                     LVGDIFDDAATGQGLLNFFLRGINCGALKEEEALRAGVTLDELRGRSFVKILKNRRGL
                     A"
     gene            complement(3591..>4005)
                     /locus_tag="VGB85_32320"
     CDS             complement(3591..>4005)
                     /locus_tag="VGB85_32320"
                     /inference="COORDINATES: protein motif:HMM:NF012684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEY0138831.1"
                     /translation="WVGDSPFRPQHRRRNWFVQVTRVQPPDDDMPTDLRPEDLELTTM
                     RASGSGGQNVNKRSTAVRARHLPSGIEVVAREERSQFANRRAALARLAWLLEDRARSR
                     RDAGARAQWDAKAQVERGNPRRVFRGPGFMPDPTS"
BASE COUNT          581 a         1381 c         1383 g          660 t
ORIGIN      
        1 cgtcaagcag tccatccagg aggtggtgaa gaccctcttc gaggccgtgg tcctggtctt
       61 cctggttatg ttcctgttcc tgcagaactg gcgcgccacc ctgatcccga ccatcgcggt
      121 gccggtggtg ctgctgggca ccttcgccgt gctgtcggcg ctcggctatt cgatcaacac
      181 cctgacgatg ttcggcatgg tgctggcgat cggcctgctc gtcgacgacg cgatcgtcgt
      241 cgtcgagaac gtcgagcgcg tgatgtccga ggacggcctg ccgcccaagg aggcgacgcg
      301 gcgctcgatg gaccagatct cgggcgcgct ggtcggcatc gccctcgtgc tcgccgccgt
      361 gttcgtgccg atggccttct tcggcggctc ggtcggcatg atctaccgcc agttctcgat
      421 caccctcgtc tcgtcgatgg cgctatcggt ggtcgtcgcg atgaccctga ccccggcgct
      481 gtgcgcgacg atgttgaagc ccggccacgg cccgcgcacg cgcggcttct tcggcctgtt
      541 caaccgcggc tacgaccgca gcagcggcgg gtatcagcgg gtgatcggcg tgctcgtgcg
      601 ccggccgttc ctggcgatgg tcggcttttt ggcgatcgtc ggcgcgctgg tgctgttgtt
      661 cgtgcgcatg ccgaccggct tcttgccgga ggaggatcag ggcgcgctca acgtgcaggt
      721 gctgctgccg ccgggcagca cgatggagcg cacgcgcgcg gtgctcgacc gggtcagcga
      781 ccacttcctc gagcaggagg gcgacgcggt ggagtcggtg ctggcgatct ccggcttcgg
      841 cttcagcggt cgcgggcaga actcgggcat cgcgttcatc cgcctcaaga actgggagga
      901 gcgcggcgac gccaggcagc gcgcgagcgc gatcgcgggt cgggcgatgg gcgccttctc
      961 gaagatcaaa gaggcgacga tcttcgccgt cgtcccgccc gcgatcagcg agctcggcaa
     1021 cgcgaccggc tttcagttcc agctgcagga ccgcggcggg ctcggccacg aggccttgat
     1081 agcttcgcgc gaccagctgc tcggcctcgc caataaacag ccggagctga cgcgcgtgcg
     1141 tcagggtggc ctcgaggaca ccccgcagta caagatcgac gtcgaccagg agaaggccgt
     1201 cgcgctcggc ctctcgctca gcgacatcaa cgcgacgctg caggcgacct ggggcgggtc
     1261 ctacgtcaac gacttcatcg accgcgggcg gaccaagcgg gtctacattc agggtgacgc
     1321 gccgtttcgc atgcagccgg acgacatcga ccgctggtac gtgcgcaaca aggacggaaa
     1381 aatggtcccg ttctcggcgt tctcgaccgg gcactggacc tacgggtcgc ccaagctcga
     1441 ccgcttcaac ggcttcccga cgatcctcat ccagggtgag gccgcgccgg gcagcagctc
     1501 gggcgccgcg atggcgacga tggagcggct cgccggcgag ctgccggcgg gcatcggctt
     1561 ccagtggtcg ggcctgtcgt acgaggaggt cgcctcgggg gccaacgcgc cgatgttgta
     1621 tgcgctgtcg ctgctggtcg tgttcctgtg cctcgcggcg ctgtacgaga gctggtcgat
     1681 ccccgcggcg gtgatgctcg tcgtgccgct cggggtcgcg ggggccgtct cggcgacgct
     1741 cggcttcggg ctcgccaacg acgtctactt tcaggtcggc ctgctgacga ccgtcggtct
     1801 ctcggcgaag aacgcgatcc tcatcgtcga gttcgccaag gagctgcgcg agcagggcaa
     1861 gggccgcctc gagtcggcgc tggaggccgc gcgcgcgcgc ctgcgaccga tcctgatgac
     1921 ctcgctcgcg ttcgtgctgg gagttcttcc gctggcgatc gccagcggcg cgggggcagg
     1981 ggcccagaac gcgatcggca tcgccgtcat cggcggcgtg ctcgcgggca ccgtgctcgg
     2041 cgtgctgttc gtgccggtgt tctacgtcgt catcgcccgc cgcgacccca agcgcgagcc
     2101 caggctcgac gcaccgatcg cccggccgcc cgcggacccc gtccacgcgt agtagcgccg
     2161 tcggcgcgga cccaaatctg cgcgccctcg tgcgcgcgga tttgggtaca ttctagccca
     2221 tgacgacgac gacctcgctg cccgcgaagg tgctgcacga cctgaccacc gccctctccg
     2281 cgacgcaggc gcggctggcc gagcgatacc cgggggagcc gggcacgcgc cagccggtgc
     2341 acaccgtcta cggcggcgcg catctgtttc gccacgacac cgcgaaaaag ctcggcgagc
     2401 tcggcctgcg tgttttgagg caatacgcgc cggacgccgc gaccttcgcg tccgcgatcg
     2461 gtctcgagag cagccacgcc gacacgatct acgagcgcgt ccttgaaaaa atgcagcgcg
     2521 aggcggtcga ggactttcgc atcgacttcg aggacggcta cggcaaccgc tccgacgccg
     2581 aggaggacgg tcacgcggtg caggccggcg aggaggtcgc gcgcggcatg aaagagggca
     2641 ccttgccgcc gtttatcggc attcgcatca agacgctctc gggcgagctg tgcgcgcggg
     2701 cgctgcggac cctcgacgtg ttcgtcacca ctgtggtccg caacagcggc gggcacctgc
     2761 cggacaactt cgtcgtcacc ctgccgaaga tcacctcgcc cgagcacgtg acgacgctgg
     2821 tcgccacctt cgaggcgctc gagagctcgc tcgcgctgct gcagggcagc ctcaagatgg
     2881 agttcatgat cgagacgacg cagtcgatcc tcgacgtcga cgggtcgatc atgctgccgc
     2941 gcatgttgac cgccgcgcag ggccgctgcg tcgccgcgca cttcggcgtc tacgacttca
     3001 ccgcctcgaa cgacatcacc gcccagtatc agcgcatggg ccaccccgcg tgcgacttcg
     3061 ccaggcacat gatgaagatg gcctacgccg gcaccggttt atggctgtcc gacggcgcga
     3121 ccaacatcat gccggtcgcg ccgcaccgcg ccgaggccgg cggcacattg accgcggagc
     3181 agaccgcgga gaaccgcaag gccgtgcacg ccgcgtggaa gctgcacgcc gacgacatct
     3241 tccattcgct cgagagtggt tattatcagg gctgggacct gcacccgggc cagctgccga
     3301 cccgctacgc tgccacctac gcgttcttct tgcgcggcct ggcatccgcc tcggagcgcc
     3361 tgtcgaactt cgtgtcgaag gcggcccagg cgacgctcgt cggcgacatc tttgatgacg
     3421 ccgcgaccgg ccagggcctg ctcaacttct tcctgcgcgg catcaactgc ggcgcgctca
     3481 aggaggagga ggcgctgcgc gccggcgtga cgctcgacga gctgcgtggg cggtccttcg
     3541 tgaagatcct caagaatcgt cgcggcctgg cctgagggac aggttgtctt tcaagaagtc
     3601 gggtcgggca taaagcctgg cccgcggaac acccggcgcg ggttgccgcg ctcgacctga
     3661 gccttggcgt cccactgggc acgcgcgccg gcgtcgcggc gcgagcgggc ccggtcttcc
     3721 agcagccagg ccaggcgcgc gagcgcggcg cgccggttgg cgaactgcga gcgctcctcg
     3781 cgggcgacca cctcgatccc gctcggcagg tgcctggccc ggacggcggt gctgcgcttg
     3841 ttgacgttct gcccgccgga cccgctggcg cgcatcgtcg tcagctcgag gtcttccgga
     3901 cgcaggtccg tcggcatatc gtcgtcgggc ggctggaccc gggtgacctg cacgaaccag
     3961 ttgcgccggc gatgctgcgg gcgaaacggg ctgtccccga cccac
//
LOCUS       DASWNJ010001939         2190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_528313, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001939 DASWNJ010000000
VERSION     DASWNJ010001939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528313"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1440
                     /locus_tag="VGB85_32325"
     CDS             <1..1440
                     /locus_tag="VGB85_32325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138832.1"
                     /translation="STGGASSSSTCDSGCDTSTSTPTTGGPVTDATSDTTTTMTGSTT
                     ETTDVTATTEDFTTTAPITCVADQECGGETPFCVDGDCVGCDSLDCSAIDPGKPVCAA
                     DLGLCVECLENADCTTVEAPACDVATATCEPCSSHDQCADTACNFETGACFPKDSLLY
                     VENTPEEPIGCSDVKPDYGGSPATPVCTLQNAFVRLVPGVATTIKLKIGTKPQNSPAA
                     LPDGDLIVAIVPEGKVKPSIVTPFAGPALTIGEGNTVFMDRIMLYNTVPTSDPLIACL
                     GGVTGARLWLEEQRVFSGRTGIRAFNCQVHVRRSTITGNTIGGIDVDGDDAALAKLWL
                     ENSFVTENNGTKFGALRLTGAVDVDILYSTIALNNSPFASIECINGWSGALTIRNSAI
                     VGADPHFGAGCGAPEVTNSFESSVTDKGELGTVFSGYAEGVFQAKIDGELEGVAIWQT
                     GDPRTDQDENLRPTNDGAEDYAGADRPVL"
     gene            complement(1441..>2190)
                     /locus_tag="VGB85_32330"
     CDS             complement(1441..>2190)
                     /locus_tag="VGB85_32330"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEY0138833.1"
                     /translation="EAVGARVTRFKPGDAVYGTCDGSFAQYACTREDRLAHKPANIGF
                     DQAAAVPVSAVTALQALQDVGRVQPGHKVLIIGAAGGVGTYAVQLAKAFGAVVTGVCS
                     TGKLELVRSIGAHEVIDYTREDFADRRGYYDLILDIAGNRTLAHLRAALAPKGTLVLV
                     GGEGGGRFFGGIGRTLRAMLLSIFVSQKLRGLMAIPRVQDLDQLTALIEAGELVPVIE
                     RTYPLAAAADAIRHMSAGHARGKLVVAVEPA"
BASE COUNT          367 a          764 c          746 g          313 t
ORIGIN      
        1 agcacgggcg gggcgtcgtc ctcgtcgaca tgtgacagcg gctgcgacac gagcacgagc
       61 acgccgacga ccggcgggcc cgtgaccgac gccacctccg acacgaccac gacgatgacg
      121 ggctcgacca ccgagaccac cgacgtcacc gcgacgaccg aggacttcac gaccaccgcg
      181 ccgatcacct gcgtcgcgga ccaggagtgc ggcggcgaga ccccgttctg cgtcgatggc
      241 gattgcgtcg gctgcgacag cctcgattgc tcggccatcg accccggcaa gccggtctgt
      301 gccgcggacc tcgggctgtg tgtcgagtgc ctggagaacg ccgactgcac gaccgtcgag
      361 gcgccggcct gcgacgtggc caccgccacc tgcgagccgt gctccagcca cgaccagtgt
      421 gcggacaccg cctgcaactt cgagaccggc gcgtgcttcc cgaaggattc gttgctttac
      481 gtcgagaaca ccccggagga gccgatcggg tgcagcgacg tgaagccgga ctacggcggc
      541 tcgccggcga cgccggtctg caccctgcag aacgcgttcg tccggctcgt cccgggcgtg
      601 gcgacgacga tcaagctcaa gatcgggacc aagccgcaga actccccggc ggcgctgccg
      661 gacggcgact tgatcgtcgc gatcgtgccc gaggggaagg tcaagccgtc gatcgtcacg
      721 ccgttcgccg gcccggcgtt gacgatcggt gagggcaaca cggtgttcat ggacaggatc
      781 atgctctaca acaccgtgcc gacgagcgat ccgctgatcg cctgcctcgg tggggtgacc
      841 ggcgcccgcc tgtggctcga agaacagcgg gtgttcagcg gtcgcacggg gatccgcgcc
      901 ttcaactgtc aggtgcacgt gcgccgctcg acgatcacgg ggaacaccat cggcggcatc
      961 gatgtcgacg gtgatgatgc cgcgctcgcc aagctgtggc tcgagaacag cttcgtcacc
     1021 gagaacaacg gcaccaagtt cggcgccctc cgcctgaccg gcgccgtcga cgtcgatatc
     1081 ctctactcga cgatcgcgct caacaacagc ccgttcgcgt cgatcgagtg catcaacggc
     1141 tggagcggcg cgctgacgat ccgcaactcc gcgatcgtcg gcgcagaccc gcactttggc
     1201 gccggttgcg gcgccccgga ggtcacgaac agcttcgagt cctccgtcac cgacaagggc
     1261 gagctcggca ccgtcttctc gggctatgcc gagggcgtct tccaggccaa gatcgacggc
     1321 gagctcgagg gggtagcgat ctggcagacc ggtgatcccc ggaccgacca ggacgagaac
     1381 ctgcgcccca ccaacgacgg agccgaggac tacgcgggcg ccgaccggcc cgtgctctga
     1441 ttacgccggc tcgaccgcca ccacgagctt gccgcgggca tgtccggcgc tcatgtgccg
     1501 gatcgcgtcc gcggccgcgg cgagcggata ggtccgctcg atgacaggca cgagctctcc
     1561 ggcctcgatc agcgcggtca gctggtcgag gtcctggacg cgtgggatcg ccatcagccc
     1621 gcgcagcttc tgcgacacga agatggagag cagcatggcg cgcagcgtgc ggccgatccc
     1681 gccgaagaag cggcctccgc cctcgccgcc gacgagcacc agcgtgcctt tcggcgcgag
     1741 agcagcgcgc aggtgcgcga gggtgcggtt gcccgcgatg tcgaggatca ggtcatagta
     1801 cccgcggcgg tccgcgaagt cctcgcgggt ataatcgatc acctcgtgtg cgccgatcga
     1861 tcgcacgagc tcgagcttgc cggtgctgca caccccggtg accaccgcgc cgaaggcttt
     1921 ggcgagctgg acggcgtacg tgccgacgcc accggcggcg ccgatgatca gcaccttgtg
     1981 accaggctgc acccggccga catcctgtaa ggcctgcaga gcggtcacgg cggagaccgg
     2041 cacggcggcc gcctggtcga agccgatgtt ggccggcttg tgtgcgagcc ggtcctcgcg
     2101 ggtacaagca tattgcgcga acgagccgtc gcaggtgccg tagacggcgt cgccgggctt
     2161 gaagcgggtc acgcgggccc cgaccgcctc
//
LOCUS       DASWNJ010001940         4295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_139176, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001940 DASWNJ010000000
VERSION     DASWNJ010001940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139176"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VGB85_32335"
     CDS             <1..323
                     /locus_tag="VGB85_32335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138834.1"
                     /translation="VHEAASGPESPAIAAPAVRAGQLAAAAGELAEAQALLERGLSLT
                     TGRKDQVDLRRAAALALARTLVGAGTPGARACALAGEAGEGLAADDPRRGEVEALRAL
                     ACTP"
     gene            complement(325..1014)
                     /locus_tag="VGB85_32340"
     CDS             complement(325..1014)
                     /locus_tag="VGB85_32340"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0138835.1"
                     /translation="MSAHEADIRASYDAVASRYAEEFADDLTDKILDRALLAAFAETV
                     RRTTTGDGSCVADLGCGPGYEARQLAGLGLDVVGVDLSPAMIAEAQRRNMPHRRLEFR
                     IGSLLALPFADAALAGAIAIYSVIHLPREERARAYHEMARVIRPGGSLLISVHTSGID
                     FPPGSIRRMEEWWGHTVALDGHFIDADEVIAGLHDAGFTIDASLERGPSTAREFPGRR
                     AYLLAMRQPTP"
     gene            complement(1024..1872)
                     /locus_tag="VGB85_32345"
     CDS             complement(1024..1872)
                     /locus_tag="VGB85_32345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138836.1"
                     /translation="MPTFTLFTVYLSSFLAAPAATDREVAPVEVVAPEAEPTPSSTPA
                     PTSDVQPESSAPPVAVKAVPANMPPPQPPPAPKKPELPIRWRVDIMLGVGTTVFRDPA
                     WRAFDRNRSALHGSLTLRADKRMGTSRLFLGGGATMRAFSAHGTPYEVFTTRISVREP
                     ILFARLSARMVQGVDLFAQLGGGISVVDVALQSTVSTSQRSVVGLADGLGGVTLYLPR
                     RWLERQRRVTGGLELGAGYTWRGDVDVRPRVDTDEDPIATDSASLGDVSLRGFTWRLG
                     VFLRFQ"
     gene            complement(1890..3464)
                     /locus_tag="VGB85_32350"
     CDS             complement(1890..3464)
                     /locus_tag="VGB85_32350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138837.1"
                     /translation="MNSPSLRPPRARGVARVCLPALLAACAPSDPFSGLYQLLPPVTT
                     RDGIVWVDREHDELLFVLPTGDTLEVRRASAGDERTIVSWSAVTRDRGAVLALTIPAT
                     AKEEDVDEQLHRFAADGTGEPVVYDVHAPFSSIALSPDHRRAVLYFGDDLGAEQLHNA
                     NQIAIVDLAGDTTQNVTLNGFGGALRAVEFPGQLTEGQPAPITIGGVAHDIVAFLASS
                     EVVLLDMDDPALDQVAIPLGTDIGFAPATTLLRPGNPLFTDPVLFVRSSAGSEVGMLT
                     LLPEAEGTGFTAQISLLSVGQSGSDFVYHDSETVPYLITADPTAGDLVFTDIRTQGGF
                     AVELAAPASRLFLRDAEAGDPGSREVVAWHPGGTKISALGLAGIEDSLGRKPRQLNIE
                     TGIESLVVLDNDRVLVGSNTRLYVVDFPADQVTPLSSQSPYDPLGSALVGERLLLGTA
                     GQQWVSTVDLETLNPESMLLDGAISSFHYLPGPKRIVVTHADPAGHLTVVDPADPARS
                     TSHVHWGFLLSGVLDR"
     gene            complement(3473..>4295)
                     /locus_tag="VGB85_32355"
     CDS             complement(3473..>4295)
                     /locus_tag="VGB85_32355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138838.1"
                     /translation="LPLPLVKGKEFTVHPVEGEYVGQTAVAESGDTMLLFTTVNPFAR
                     TAGPPRQLATPEPGPEFASTGGDSTSGDSSSGDSTSGDSGDSTSGDSTGDDDTTTGDL
                     PHDDPRQRITLARREGGEWSLVTLFVEVPVASVGISPDSRNAILVHAQDPGSPAAAWS
                     YSLFDLTPKFPLLKRQTTQAAPGPVLFTPDGARAAVLLRDDPSNIRKVDLVDLNNFIV
                     APLLLGSPPEGAGYVEATGKIFVSQEHPTGRITFVGAMGQVQTVTGYVLNDSVKD"
BASE COUNT          592 a         1403 c         1699 g          601 t
ORIGIN      
        1 ccgtgcacga ggccgccagc gggcccgaga gccccgcgat cgcggccccg gcggtgcgcg
       61 ccgggcagct cgcggcggcc gcgggggagc tcgccgaggc gcaggcgctg ctcgagcgcg
      121 gcctgtccct gacgacaggt cgcaaggacc aggtcgacct gcggcgcgcc gccgcgctcg
      181 cgctcgcgcg gacgctcgtc ggcgcgggca cgccgggggc acgcgcctgc gcgctcgcgg
      241 gcgaggccgg cgaggggctc gcggcggacg acccgcggcg cggcgaggtc gaggcgctgc
      301 gggccctcgc gtgtacgccg tgagctacgg ggtcggctgg cgcatggcga gcaggtaggc
      361 gcggcggccc gggaactcgc gggcggtgct cgggccgcgc tcgaggctgg cgtcgatcgt
      421 gaagccggca tcgtggaggc cggcgatcac ctcgtcggcg tcgatgaagt ggccgtcgag
      481 cgcgacggtg tggccccacc actcctccat gcggcggatg ctgcccggcg ggaagtcgat
      541 cccggaggtg tgcacggaga tcagcaggga gccgccgggg cggatcacgc gggccatctc
      601 gtggtaggcg cgagcgcgct cctcgcgggg caggtggatg accgagtaga tggcgatcgc
      661 gccggcgagc gcggcgtcgg cgaagggcag ggccagcagc gagccgatgc ggaactcgag
      721 gcgccggtgc ggcatgttgc ggcgctgggc ctcggcgatc atcgccggcg agaggtcgac
      781 gccgacgacg tcgaggccga ggccggcgag ctggcgggcc tcgtagccgg gcccgcagcc
      841 gaggtcggcg acgcagctgc cgtcgccggt ggtcgtgcga cgcacggtct cggcgaacgc
      901 agcgagcagc gcgcggtcga ggatcttgtc ggtgaggtcg tccgcgaact cctccgcgta
      961 gcgactggcg accgcgtcgt agctggcacg gatatcggct tcgtgggcgc tcactcccac
     1021 atattactgg aagcggagga acacgccgag tcgccaggtg aagccgcgca gggagacgtc
     1081 gcccaggctt gcgctgtcgg tcgcgatcgg gtcctcgtcg gtgtcgaccc ggggccgcac
     1141 gtcgacgtcc ccacgccagg tgtagccagc ccccagctcg agcccgccgg tcacccggcg
     1201 ctgccgctcg agccagcgcc gcggcaggta cagggtgacg cccccgaggc cgtccgccag
     1261 cccgacgacg ctgcgctggg aggtcgagac ggtggattgc agggcgacgt cgacgacgct
     1321 gatgccgccg ccgagctggg cgaacaggtc gacgccctgg accatccgcg ccgacagccg
     1381 cgcgaacaag atcggctcgc gcacgctgat ccgcgtcgtg aacacctcgt acggggtccc
     1441 gtgggccgag aaggcccgca tcgtcgcccc gccgccgagg aacagcctgc tggtgcccat
     1501 gcgcttgtcg gcgcgcaggg tcaggctgcc gtgtagtgcg ctgcgatttc tgtcgaacgc
     1561 gcgccaggcg gggtcgcgga acacggtggt cccgaccccg agcatgatgt ccacgcgcca
     1621 gcggatcggc agctcgggct tcttcggcgc cggcgggggc tgcggtgggg gcatgttcgc
     1681 ggggacggcc ttgacggcga ctggcggcgc gctggactcg ggttgcacgt cactcgtcgg
     1741 cgcgggcgtt gatgatggtg tcggctccgc ctcgggcgcg acgacctcga cgggggcgac
     1801 ctcgcggtcg gtcgcggccg gggcggcgag aaatgacgat agataaactg tgaacaatgt
     1861 gaaggtcggc atagtatcga tgtctccctt cagcggtcga gcacgccgct cagcaagaag
     1921 ccccagtgga cgtggctcgt cgagcgcgcg gggtcggcgg ggtcgacgac ggtgaggtgt
     1981 ccggccgggt cggcgtgggt gacgacgatc cgcttcgggc ccgggaggta atgaaagctg
     2041 gagatcgcgc cgtcgagcag catcgactcg gggttgaggg tctcgaggtc gaccgtgctg
     2101 acccactgct ggccggcggt gccgagcagc aggcgttcgc cgaccagcgc cgagccgagc
     2161 gggtcgtagg gcgactgcga ggacagcggc gtgacctggt cggccgggaa gtcgacgacg
     2221 tagagccgcg tgttcgagcc gacgagcacg cggtcgttgt cgagcacgac gagcgactcg
     2281 atgccggtct cgatgttgag ctgccgcggt ttgcggccga ggctgtcctc gatgccggcg
     2341 agcccgagcg cgctgatctt ggtgccgccc gggtgccagg cgacgacctc gcgggagccg
     2401 gggtcgccgg cctcggcgtc gcgcaggaac aggcggctgg ccggggcggc gagctcgacc
     2461 gcgaagccgc cctgcgtgcg gatatcggtg aacacgaggt cgcccgcggt cgggtccgcg
     2521 gtgatcagat acgggacagt ctcgctgtcg tgatagacga agtcgctgcc gctctggccg
     2581 accgagagca ggctgatctg cgcggtgaag ccggtgcctt cggcctcggg tagcagcgtc
     2641 agcatgccga cctccgagcc cgcgctcgag cgcacgaaca gcaccgggtc ggtgaacagc
     2701 gggttgccgg ggcgcagcag ggtggtcgcc ggcgcgaagc cgatgtccgt gccgagcggg
     2761 atcgccacct ggtcgagcgc cgggtcgtcc atgtcgagca acacgacctc gctggacgcg
     2821 aggaaggcga cgatgtcgtg ggcgaccccg ccgatcgtga tcggcgcggg ctggccctcc
     2881 gtcaactggc cggggaactc gacggctcgc agcgcgccgc cgaagccgtt gagcgtcacg
     2941 ttttgcgtgg tgtcgccggc gaggtcgacg atcgcgatct ggttggcgtt gtgcagctgc
     3001 tccgcgccga ggtcgtcgcc gaagtagagc acggcgcggc ggtggtcggg gctgagcgcg
     3061 atcgacgaga agggcgcgtg cacgtcgtag acgaccggct cgccggtgcc gtcggcggcg
     3121 aagcggtgca gctgctcgtc gacgtcctcc tccttggcgg tcgcggggat cgtcagcgcc
     3181 agcacggcgc cgcgatcgcg ggtcaccgcg ctccacgaca cgatcgtgcg ctcgtcgccg
     3241 gcgctggcgc ggcgcacctc gagggtgtcg ccggtcggca agacgaacag cagctcgtcg
     3301 tgctcgcggt cgacccagac gatgccgtcg cgggtcgtga ccgggggcag cagctggtac
     3361 aggccggaga aggggtcgga gggcgcgcag gcggcgagca gcgccggcag gcacacccgt
     3421 gcgacgccgc gggctcgcgg ggggcggagg cttgggctgt tcatgttaat gattaatcct
     3481 tgaccgagtc gttgagcacg tagccggtca ccgtctggac ctggcccatc gcgccgacga
     3541 aggtgatgcg tccggtcggg tgttcctgcg acacgaagat ctttccggtc gcctcgacgt
     3601 agccggcgcc ctcgggcggc gagccgagca gcagcggggc gacgatgaag ttgttgaggt
     3661 cgacgaggtc gaccttgcga atgttgctcg gatcgtcgcg cagcagcacg gccgcgcgcg
     3721 cgccgtcggg cgtgaacagc accgggcccg gcgcggcctg ggtggtctga cgcttgagca
     3781 gcgggaactt gggggtcagg tcgaacaggc tgtacgacca cgccgccgcc gggctccccg
     3841 ggtcctgcgc gtgcacgagg atcgcgttgc ggctgtcggg cgagatgccg acgctcgcca
     3901 ccggcacctc gacaaacagc gtcaccagcg accactcgcc gccctcgcgc cgcgcgagcg
     3961 tgatgcgctg gcgcgggtcg tcgtgcggga ggtcgccggt ggtcgtatca tcgtcgccgg
     4021 tcgaatctcc ggaggtcgag tcgccgctat ctccggaggt ggaatctccg gagctcgaat
     4081 cgcccgaggt ggaatctccg ccggtcgacg cgaactcggg tccgggctcg ggggtcgcga
     4141 gctggcgagg cggtccggcc gtgcgcgcga acgggttgac ggtggtgaac agcaacatgg
     4201 tgtcgccgct ctcggcgacc gcggtctggc cgacgtactc gccctcgacc gggtggaccg
     4261 tgaattcctt gcccttgacc agcggcagcg gcagc
//
LOCUS       DASWNJ010001941         1638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_416901, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001941 DASWNJ010000000
VERSION     DASWNJ010001941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416901"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VGB85_32360"
     CDS             <1..365
                     /locus_tag="VGB85_32360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138839.1"
                     /translation="PLTVAAKDTAQGHAFEIRARPNSGEFVVATMVFDPEWSYLGHYN
                     GVLGGGASTVSRPLPASPLSSPPPPAVIAPVAAATPTVTPTAAVAEAAAASPTPTPAG
                     VQAVPAGSAQSPGLAGTP"
     gene            complement(381..740)
                     /locus_tag="VGB85_32365"
     CDS             complement(381..740)
                     /locus_tag="VGB85_32365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138840.1"
                     /translation="MKFISPQQHALLDFVSWFVFLLAPSHFEAGAPGSAAIGCYLTAI
                     AGFLVCMCTRYPLGIVPLMSFRTHGRLELAYVPVLLIWPWLAGFGQFEVARGFFIATG
                     LALLLLWMGTDYEAPQR"
     gene            complement(828..>1638)
                     /locus_tag="VGB85_32370"
     CDS             complement(828..>1638)
                     /locus_tag="VGB85_32370"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEY0138841.1"
                     /translation="VDGVVQPPLATSPGEQTYPLAQGLPPGEHTVELYRRTEGSFGST
                     VLLGVDVEGELLAPPPVQRRIEIIGDSITCGYGNEGADPCNFSAETENHYLTYGAVAA
                     RTLGAEISAVCWSGKGVVNNYGDDVNEPMPQIYDRLLATDGAPWSFAWQPDVVVINLG
                     TNDFSTDNDPAEGVFVPAYVQFLAHLRDVYPDAFILAIAPSLWGAEAEMVAGYLQSAV
                     DQRHGEGDPDVAFASVNVDWIGSGCNGHPSVATHAAMGAKLAETLQTQLGW"
BASE COUNT          247 a          592 c          569 g          230 t
ORIGIN      
        1 acccgctgac ggtcgccgcc aaggacaccg cgcagggcca cgccttcgag atccgggccc
       61 gcccgaacag cggcgagttc gtggtcgcca cgatggtctt cgaccccgag tggagctacc
      121 tcggccacta caacggcgtg ctcggcggcg gcgcatcgac cgtgtcccgg cccctgcccg
      181 cgtcgccgct gtcgtcgccg ccgccgcccg ctgtcatcgc gccggtcgcc gccgccacgc
      241 ccacggtcac gccgaccgcg gccgtcgccg aggccgccgc tgcgagcccg acgccgaccc
      301 ccgcgggggt gcaagccgtg cccgccggca gcgcgcagag cccgggcctc gccggcacgc
      361 cctgagcgaa ccgccggcgc tcagcgctgg ggcgcctcgt aatcggtgcc catccacagc
      421 agcagcagcg ccaggccggt ggcgatgaag aagccgcgcg cgacctcgaa ctggccgaag
      481 cccgcgagcc acggccagat cagcagcact ggtacgtatg ccagctcgag tcgtccgtgc
      541 gttcgaaatg acatcaacgg gacgatgccg agcgggtaac gggtgcacat gcacacgagg
      601 aaccccgcga tcgccgtcag gtaacagccg atcgccgcgc tgcctggcgc gcccgcctcg
      661 aagtggctgg gcgcgagcag gaacacgaac cacgacacga agtcgaggag agcgtgctgc
      721 tgtggggaga tgaacttcat gggctcacct cgatgtatgc gcgtgtcgca ggaaggtgca
      781 acccgctcac gggatctgga tcaccgttcg ctgcccgcgg aagggcgtca ccagccgagt
      841 tgtgtttgca acgtctccgc gagcttggcg cccatcgccg cgtgggtcgc cacggacggg
      901 tgtccgttgc agccggatcc gatccagtcg acgttgacgc tggcgaacgc gacgtcgggg
      961 tcgccctcgc catggcgctg atcgacggcg ctctgcagat agccggcgac catctctgcc
     1021 tcggcgcccc acaggctcgg cgcgatcgcg aggatgaacg cgtcgggata gacgtcgcgc
     1081 aggtgggcga ggaactgcac gtacgcgggc acgaacacgc cctcggcggg atcattgtcg
     1141 gtgctgaagt cgttggtgcc gaggttgatc accacgacgt cgggctgcca cgcgaagctc
     1201 cacggcgcgc cgtcggtggc gagcaggcgg tcgtagatct gcggcatcgg ctcgttgacg
     1261 tcgtcgccgt agttgttgac cacgcccttg cccgaccagc acaccgcgct gatctccgcg
     1321 ccgagcgtgc gcgccgcgac ggccccgtag gtcaggtagt ggttctcggt ctccgcgctg
     1381 aagttgcacg ggtcagcgcc ctcgttgccg tagccgcacg tgatcgagtc gccgatgatc
     1441 tcgatccggc gctgcaccgg cggcggcgcg agcagctcgc cctcgacgtc gacgccgagc
     1501 agcaccgtcg acccgaacga cccctcggtg cggcggtaca gctcgaccgt gtgctcgccc
     1561 ggcggcaggc cctgcgcgag cgggtaggtc tgctcgccgg ggctcgtcgc cagcggcggc
     1621 tgcacgaccc cgtcgacg
//
LOCUS       DASWNJ010001942         1513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_599730, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001942 DASWNJ010000000
VERSION     DASWNJ010001942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599730"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="VGB85_32375"
     CDS             complement(<1..817)
                     /locus_tag="VGB85_32375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138842.1"
                     /translation="MHEPASHERPSLTPLSCLACGRPVALGDGDTARCRACGVEVEIP
                     GPYLALRAAVRSSDQARARAEAISAELARPQSLFVRFWMTAGDLAVTFTTLLVVVWIG
                     VSLVMCIGTIFSEGLLAGLIALGIGFVLGAPLLYNEALHALAEPLGVDLADVWGGAGS
                     YALLGLACWIVSVVPQALAAYAAMFESVRTALRTALAADPPAVPGGAEQCRTCGAPLD
                     DRAGASHVRCIYCGADNLVLLDAARLSRLEAGVKAACTDLESASAEEAKAARRG"
     gene            complement(828..>1513)
                     /locus_tag="VGB85_32380"
     CDS             complement(828..>1513)
                     /locus_tag="VGB85_32380"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY0138843.1"
                     /translation="AIVLCTDAGGQRPALLEIAGRLADLGYLVAIPDLYFRAGKLQDL
                     LPEGQKDRPIITAIFGDAAVRKQWYQRFYASATADETVRVGIGAVLDHLAARPDVTGK
                     VGTTGYCMGGNISLRIATLFGDRIAATASFHGGGLVTAAPDSPHLRAAAIRSRVYVAA
                     ASEDETFNDEARRTLEAALTEAGVEHTIEVYPGRHGFAVTDNPTHDAACAARHFAALE
                     SFYAATLRA"
BASE COUNT          209 a          540 c          582 g          182 t
ORIGIN      
        1 ggccgcgccg cgccgccttc gcctcctcgg cgctcgccga ctcgaggtcg gtgcaggccg
       61 ccttcacccc ggcctcgagt cgcgacaggc gcgccgcgtc gaggaggacg aggttgtcgg
      121 cgccgcagta gatgcagcgc acgtgcgacg cgccggcccg gtcgtcgagc ggtgcgccgc
      181 aggtccggca ctgctccgcg ccgccgggga ccgccggggg gtcggccgcg agcgcggtcc
      241 gcagcgcggt gcggaccgac tcgaacatcg cggcgtacgc ggcgagggcc tgcggcacca
      301 cgctgacgat ccagcacgcg aggccgagca gcgcgtacga gccggcccct ccccacacgt
      361 cggcgaggtc gacccccagc ggctccgcga gcgcgtgcag ggcctcgttg tagagcagcg
      421 gagcgccgag cacgaagccg atgccgagcg cgatcagccc ggcgagcagg ccctcgctga
      481 agatggtgcc gatgcacatc accagcgaca cgccgatcca caccacgacc agcagcgtcg
      541 tgaacgtgac cgcgaggtcg ccggcggtca tccagaagcg cacgaacagc gactgcggcc
      601 gcgcgagctc ggcgctgatc gcctccgcac gcgcgcgcgc ctggtcgctc gaccggaccg
      661 cggcgcgcag ggcgaggtat ggcccgggga tctcgacctc gacgccgcac gcccggcagc
      721 gggccgtgtc accgtcgccg agcgcgaccg gccgaccgca cgcgagacag ctcagcggcg
      781 tcagcgacgg gcgctcgtga ctcgcgggct cgtgcatcgg tcggcctcta cgcgcgcagg
      841 gtcgcggcgt agaaggactc gagggcggcg aagtgccgcg ccgcgcaggc cgcgtcgtgg
      901 gtcgggttgt cggtcaccgc gaagccgtgt cggccggggt agacctcgat cgtgtgctcg
      961 acgccggcct cggtgagggc ggcctcgagg gtgcgccggg cctcgtcgtt gaaggtctcg
     1021 tcctcgctcg cggccgcgac gtagacgcgc gagcggatcg cagctgcgcg caggtgcggg
     1081 ctgtcgggcg ccgcggtgac caggccaccg ccgtggaacg aggccgtcgc ggcgatgcgg
     1141 tcgccgaaca gcgtggcgat gcgcagcgag atgttgccgc ccatgcagta gccggtggtg
     1201 ccgaccttgc cggtcacgtc cggccgggcg gcgaggtggt cgagcaccgc gccgatgccg
     1261 acgcgcacgg tctcgtcggc ggtggcggac gcgtagaagc gctggtacca ctgctttcgc
     1321 accgccgcgt cgccgaagat cgccgtgatg atcggccggt ccttttggcc ctcgggcagc
     1381 aggtcctgga gcttgccggc ccgaaaatag aggtcgggga tggcgacgag atagccgagg
     1441 tcggcgagcc gcccggcgat ctcgagcagc gccggacgct gcccgccggc atcggtgcac
     1501 aggacgatcg cag
//
LOCUS       DASWNJ010001943         3748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_105650, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001943 DASWNJ010000000
VERSION     DASWNJ010001943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105650"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            420..2855
                     /gene="lon"
                     /locus_tag="VGB85_32385"
     CDS             420..2855
                     /gene="lon"
                     /locus_tag="VGB85_32385"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEY0138844.1"
                     /translation="MTHPELPKSPFPLLPLRNGVLFPGTVITVPVGRARSIALIESLE
                     PGKSILGIAVQRDARVVDPQLADLQSVGTYARVRQVRRTGDRNYQVVLEGLGRFKLGN
                     IVQSSPFWMVEGEALPDLVTNPAEARALAEALGGQVREQLQEVAPDVVANFSQWIETL
                     GRADDPGLLADRVAAALGLDTDKEVGVLMTREVGERLRLVTRLLIEARTMHDLRKKIE
                     SEVRKGLNSGQREVLLRQQMRAIQKELGEEPKSDDLAALRERLDKAELPEEVRTVADK
                     ELRRLDSMNSAQAEYNVIRTYLEWISELPWSTRADDEIDLNKVAAKLDADHYGLDEVK
                     KRILEHMAVLKVSGTARATILCLAGPPGVGKTSLGQSIADATGRPFVRIALGGVRDEA
                     EIRGHRRTYVGALPGRLIHALKKAGKKNAVILLDEIDKLGQSWMGSPEAALLEVLDPE
                     QNKTFTDHYLELPFDLSEVTFIVTANSLETLSAPLRDRLEIIEVSGYTAEEKLHIGKQ
                     HLLPKQLAIHAIAEGTLRVSDEAMRAIIRDYTREAGVRQLTRELTKLCRALTLEFARS
                     SDEKPARLAIEPADLNKYLGKVRFISEVAERTAVPGVATGLAWTPVGGDILFIETSRM
                     PGKGHLEITGQLGDVMKESARAALTYVRSNADTLGIDAGFLNTQDLHIHVPAGAVPKD
                     GPSAGVTIFTALTSLLTGRRVRPDTAMTGECTLRGRVLPVGGIKSKVLAAHRAGLTRV
                     ILPQRNARDIDDVPADVRNQMEFIFAEDMAQVLAAALEPNLLGAKPKGDLDPHEETAE
                     LATNKGETPRAQA"
     gene            3126..>3748
                     /locus_tag="VGB85_32390"
     CDS             3126..>3748
                     /locus_tag="VGB85_32390"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0138845.1"
                     /translation="MLERISAGGMAEVYRAKQTGADNFERPVAIKRILPHIARDPNFI
                     AMFQAEAKLAVQLQHGNICQIYQLGRHEDSFYIALEYVDGRDVGAILDLHQKQNRPIP
                     LPQACYIIRRVCEGLDYAHNKKSTQGEPLHIIHRDISPPNLLISYEGEVKLIDFGLAK
                     AVGSSVQTQAGIIKGKLAYMSPEQVRGAPLDHRSDVFAVGIVFFELLT"
BASE COUNT          633 a         1417 c         1163 g          535 t
ORIGIN      
        1 cagacccgcc caaaaagcgg gtcaaagacc gcggcgcaca gtgtcctatt cacggccccg
       61 tgaatcaatc ttcacgcccg gtcggatccg cccgatcggg cgatgtcgtc tgcgaccccg
      121 ctcacgtgtc tgccaccgga agaccaccac gcggtcatcg cccccgcggt cgataacctg
      181 tcctttcaga cagcctggcc ccgctccccc gactcgacgc agcccctcta gcacgcctcc
      241 ggtggccatc tgaccgccac aaacgcagtt cttggccgag ttctttttcc tgcgcgctgg
      301 ttgacggacc ggcgaacggt tttacgttca acaaacccgc cggcccgtgt gtgtggctgg
      361 cgagcgtggc tgcgacaaca gccccggcag gcgcgacgcc gccgggcgcg aggaaaacta
      421 tgactcatcc agaactgccc aagtctccgt ttccgctgct gcccctgcgc aacggcgtcc
      481 tcttcccggg cacggtcatc accgtcccgg tcggtcgcgc ccgctcgatc gcgctgatcg
      541 agtcgctcga gcccggcaag tcgatcctcg gcatcgccgt ccagcgcgac gcccgcgtgg
      601 tcgaccccca gctcgccgac ctacagtccg tcggcaccta cgcccgcgtc cgccaggtcc
      661 gccgcaccgg cgaccgcaac tatcaggtcg tgctcgaggg cctcggccgg ttcaagctcg
      721 gcaacatcgt ccagtcgtcc ccgttctgga tggtcgaggg cgaggccctg cccgacctcg
      781 tcaccaaccc cgcggaggcc cgcgcgctcg cggaggcgct cggcggccag gtccgcgagc
      841 agctgcagga ggtcgccccg gacgtcgtcg ccaatttttc gcagtggatc gagacgctcg
      901 gccgcgccga cgaccccggc ctgctcgccg accgcgtcgc cgccgctctc ggcctcgaca
      961 ccgataaaga agtcggcgtg ctgatgaccc gcgaggtcgg cgagcgcctg cgcctcgtca
     1021 cccgcctcct catcgaggcg cgcacgatgc atgacctgcg caagaagatc gagtccgagg
     1081 tccgcaaggg cctcaactcc ggccagcgtg aggtcctgct gaggcagcag atgcgcgcga
     1141 tccagaagga gctcggcgag gagccgaagt ccgacgacct cgccgccctg cgcgagcgcc
     1201 tcgacaaggc cgagctgccc gaggaggtcc gcaccgtcgc cgacaaggag ctgcgccgcc
     1261 tcgacagcat gaacagcgcg caggccgagt acaacgtcat ccgcacctac ctcgagtgga
     1321 tctccgagct cccgtggagc acccgcgccg acgacgagat cgacctcaac aaggtcgcgg
     1381 ccaagctcga cgccgaccat tacggcctcg acgaggtcaa gaagcgcatc ctcgagcaca
     1441 tggcggtcct caaggtctcg ggcaccgccc gcgcgaccat cctgtgcctc gccggccctc
     1501 cgggcgtcgg caagacctcg ctcgggcagt cgatcgccga cgccaccggc cggcccttcg
     1561 tgcgcatcgc cctcggcggc gtgcgcgacg aggcggagat ccgcggccac cgtcgcacct
     1621 acgtcggcgc gctgcccggc cgcctgatcc acgcgctcaa gaaggccggc aagaagaacg
     1681 cggtcatcct gctcgacgag atcgacaagc tcggccaaag ctggatgggc tcgccggagg
     1741 ccgccctgct cgaggtcctc gaccccgagc agaacaagac cttcaccgat cactacctcg
     1801 agctgccctt cgacctctcc gaggtcacct tcatcgtcac cgccaacagc ctcgagacgc
     1861 tgtcggcgcc gttgcgggac cgcctcgaga tcatcgaggt ctccggctac accgcggagg
     1921 agaagctgca catcggcaag cagcacctgc tgcccaagca gctcgccatc cacgcgatcg
     1981 ccgagggcac gttacgggtc agtgacgaag ccatgcgcgc gatcatccgc gactacaccc
     2041 gcgaggccgg cgtccgccag ctgacccgcg agctgaccaa gctgtgccgc gcgctcaccc
     2101 tcgagttcgc ccgcagcagc gacgagaagc cggcgcgcct cgccatcgag cccgccgacc
     2161 tgaacaagta cctcggcaag gtgcgcttca tcagcgaggt cgccgagcgc accgcggtgc
     2221 cgggcgtggc gaccggcctg gcctggacgc cggtgggcgg ggacatcctg ttcatcgaga
     2281 cctcgcggat gcccggcaag ggccacctcg agatcaccgg acagctcggg gacgtgatga
     2341 aggagtcggc ccgcgcggcg ctcacctacg tgcgcagcaa cgcggacacg ctcggcatcg
     2401 acgccggctt cctcaacacc caggacctgc acatccacgt gccggccggc gccgtgccca
     2461 aggacggccc ctccgcgggc gtgacgatct tcaccgcgct cacctcgttg ttgaccggac
     2521 gccgcgtccg tcccgatacc gcgatgaccg gcgaatgcac gctgcgcggt cgcgtcctcc
     2581 cggtcggcgg catcaagtcg aaggtcctcg ccgcccaccg ggccggcctg acgcgggtga
     2641 tcctcccgca gcgcaacgcg cgtgacatcg acgacgtgcc cgccgacgtc cgcaaccaga
     2701 tggagttcat cttcgccgag gacatggcgc aggtgctggc cgccgcgctc gagcccaacc
     2761 tgctcggcgc caagcccaag ggcgacctgg acccccacga ggagacggcc gagctcgcca
     2821 cgaacaaggg cgagaccccc cgcgcgcagg cctgagcgcg ggccccgaca gacgcgagcg
     2881 accgcccgcc ctggcacacg ccggggcggg cggtcgcgtt tggctcgcca tttttggcca
     2941 tcctccacga ttttcgcgtc gggatcgtcc cgcgcagcgc cggctccggg cgcgaaagat
     3001 cgcgggtttt gctcggcgaa acgaccgtcc ggcgcgcgtt cgcccgcgtg ctcgcgttga
     3061 cttgctggaa ggacagcgat taactgggcg tgttggcggt acccgaccga atcggtcctt
     3121 atcaggtgct cgagcgcatc agcgccggcg gcatggccga ggtgtaccgc gccaagcaga
     3181 ccggcgccga caacttcgag cgaccggtgg cgatcaagcg gatcttgccg cacatcgccc
     3241 gcgacccgaa cttcatcgcg atgttccagg ccgaggccaa gctcgcggtc cagctgcagc
     3301 acggcaacat ctgccagatc taccagctcg gtcgccacga ggactccttc tacatcgccc
     3361 tcgagtacgt cgacggccgt gacgtcggcg ccatcctcga cctgcatcag aagcagaacc
     3421 ggccgatccc gctgccgcag gcctgttaca tcatccgccg cgtgtgcgag ggcctcgact
     3481 acgcgcacaa caagaagagc acccagggcg agccgctcca catcatccac cgcgacatca
     3541 gcccgccgaa cctgctgatc tcctacgagg gtgaggtcaa gctgatcgac ttcgggctcg
     3601 ccaaggcggt cggctcgtcg gtccagaccc aggccggtat catcaaaggc aagctcgcgt
     3661 acatgtcgcc cgagcaggtc cgcggcgccc cgctcgatca ccgcagcgat gtgttcgccg
     3721 tcggcatcgt gttcttcgag ctgctgac
//
LOCUS       DASWNJ010001944         1958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_34480, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001944 DASWNJ010000000
VERSION     DASWNJ010001944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34480"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="VGB85_32395"
     CDS             <1..987
                     /locus_tag="VGB85_32395"
                     /inference="COORDINATES: protein motif:HMM:NF021285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HEY0138846.1"
                     /translation="PGTSDELEALLADLGRYNDPALLAEPDRLLAPPPAAPRLAVLER
                     RPIADGRATHYSFASAYRPLHPTYAREYAGYDRLDTVHVHAWEHARPAPASIILVHGW
                     AMGARRLHELEFSVASLFRDLGLDVYFYVQPFHGERRPSQARYNGQLFPSTDLVRTNE
                     AFIQTCMDLRALLGAVLERRGGRVGMMGSSLGGYTSALIASVDPRLDFVVPIMAPASM
                     AHLIWDHGAGDPFRTRAEGAGMTRDRFHHAWALHSPLSYQPRVPWERRLIVGAADDVL
                     VRPTDVDVLWEHWQRPRRFTFAGGHILQLGRRAYIRELARFLDDLGVIHSRL"
     gene            1159..>1958
                     /locus_tag="VGB85_32400"
     CDS             1159..>1958
                     /locus_tag="VGB85_32400"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0138847.1"
                     /translation="MANYLADNDDLLFYLERGIDWAPIVALVEHNFQAPQGYTSTEEA
                     VAAYRDMLEMVGNFAADEVAPYAPVIDRKGVKLVDGEAVLPPELDTIFAKMKDLDLHS
                     LSIPRELGGVNCPMLVHFVVTELLARGDVSVMAHYNFHSGIALSLLMYSVNEGTTTFD
                     RATHSISGTRFAAEIAEILRGDAFGSMDITESDAGSDMGALKCKGEQDEHGNWFVSGQ
                     KIFITSGHGKYHIVIARTEPAGAGLGALGLEGLSLFLVPAYEDLPDGTR"
BASE COUNT          283 a          778 c          599 g          298 t
ORIGIN      
        1 cccggcacct ccgacgagct cgaggcgctg ctcgccgacc tcggccgcta caacgaccct
       61 gcgctgctcg ccgagcccga ccgcctgctc gcgccgcccc ccgccgcgcc gcgcctcgcc
      121 gtgctcgagc gccgcccgat cgccgacggc cgcgccaccc actacagctt cgcctcggcc
      181 taccgcccgc tgcacccgac ctacgcccgc gagtacgccg gctacgatcg cctcgacacc
      241 gtgcacgtgc acgcgtggga gcacgcccgc cccgcgcccg cctcgatcat cctcgtccac
      301 ggctgggcga tgggcgcccg ccgcctgcac gagctcgagt tcagtgtagc ctcacttttt
      361 cgcgacctcg gcctcgacgt ctacttctac gtgcagccct tccacggcga gcgcaggccc
      421 tcgcaggcgc gctacaacgg tcagctgttc ccctcgaccg acctcgtgcg caccaacgag
      481 gccttcatcc agacctgcat ggacctgcgc gcgctgctcg gcgcggtgct cgagcgtcgc
      541 ggcggccgcg tgggcatgat gggctcgagc ctcggtggtt acacctcggc gctgatcgcc
      601 tcggtcgacc cgcgcctcga cttcgtcgtc ccgatcatgg cgcccgcctc gatggcccac
      661 ctgatctggg accacggcgc cggcgacccg tttcgcaccc gcgccgaggg ggccggcatg
      721 acccgcgacc gcttccatca cgcgtgggcc ctgcactcgc cgctgagcta ccaaccgcga
      781 gtgccctggg agcggaggtt gatcgtcggg gccgccgacg acgtgctcgt gcgcccgacc
      841 gacgtcgacg tcttgtggga gcactggcag cgcccgcgcc gcttcacctt cgccggcggc
      901 cacatcctgc aattgggccg ccgcgcctat atccgcgagc tcgcccgctt cctcgatgac
      961 ctcggcgtca tccactcccg gctctaaaaa agcgggcgcc ggcgcgcacg cgcgctcgcc
     1021 gggaacccgg ccgccttggc tcacgggcgc agccgcgaac gctgcgcttg cgccgcgcgc
     1081 gagggatcgc taatctggcg aaactgcgcg ggccacgggt ccgcgttgaa catcgttcat
     1141 ccgcccgcag gtgcatccgt ggccaactat ctcgcagaca acgacgacct tctcttctac
     1201 ctcgagcgag gcatcgactg ggcgccgatc gtggcgctgg tcgagcacaa cttccaggcc
     1261 ccgcagggtt acacgagcac cgaggaggcc gtcgctgcgt accgcgacat gctcgagatg
     1321 gtcggcaact tcgccgccga cgaggtcgcc ccatacgctc cggtgatcga ccgcaagggc
     1381 gtcaagctcg tcgacggcga ggccgtgttg ccgcccgagc tcgacacgat cttcgcgaag
     1441 atgaaggacc tcgacctgca ctcgctgtcg atcccgcgcg agctcggcgg cgtcaactgc
     1501 ccgatgctcg tccacttcgt cgtcaccgag ctgctcgccc gcggcgacgt ctcggtgatg
     1561 gcccactaca acttccacag cggcatcgcg ctcagcctgc tgatgtactc ggtcaacgag
     1621 ggcaccacca ccttcgaccg cgccacgcat tcgatcagcg gcacccgctt cgccgccgag
     1681 atcgcggaga tcctgcgcgg cgacgccttc ggcagcatgg acatcaccga gtccgacgcc
     1741 ggcagcgaca tgggcgccct caagtgcaag ggcgagcagg acgagcacgg caactggttc
     1801 gtctccggcc agaagatctt catcacctcg ggccacggca agtaccacat cgtcatcgcc
     1861 cgcaccgagc ccgccggcgc cggtctcggg gctctcgggc tcgagggcct ctcgctgttc
     1921 ctcgtcccgg cctacgagga cctcccggac ggcacccg
//
LOCUS       DASWNJ010001945         2436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_345484, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001945 DASWNJ010000000
VERSION     DASWNJ010001945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345484"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VGB85_32405"
     CDS             complement(<1..836)
                     /locus_tag="VGB85_32405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138848.1"
                     /translation="MTRPSRPVPAACLALLTLLAACGDDTGTSSAGSTGTTDPASSST
                     AEPGTTSADPPTTGTGTTEAGSADGGATESPTTSPGTTGNDTIPGTATGSTSTTGSTT
                     DETTGEDTTTGVMPLEHFLLGVHVQDVSPSPEVLSGKDVYMGAYGAPYTRGPAQGVHD
                     PIFARSFAVEGNGGGIIMSIVDLPGMGNRITRDVRTKVAALTGLDEAQVLIGSTHSHS
                     APDFMGLWGGVPGDYRTWLIDQVSASMAAAWDARVPGELRVSTGKAPANNRRGWNFTD
                     DD"
     gene            complement(930..1217)
                     /locus_tag="VGB85_32410"
     CDS             complement(930..1217)
                     /locus_tag="VGB85_32410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138849.1"
                     /translation="MAAIMIDYATLCRAIEDWKSGRQPSAVTPAAPRRIAAPAQEDEE
                     AVEYSAVYDTNDLPAEQPGPDPTGVYQLSEYEGDVDADVDADEVDVTDDER"
     gene            complement(1257..1913)
                     /gene="tmk"
                     /locus_tag="VGB85_32415"
     CDS             complement(1257..1913)
                     /gene="tmk"
                     /locus_tag="VGB85_32415"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEY0138850.1"
                     /translation="MAETPRGCFIVLEGIDGSGTTLQTRALADRLRERGREVLETREP
                     TSGAIGALIRERLSVRAAALDPAALALLFAADRLDHLTREVIPAVAAGKVVISDRYLL
                     SSLAYQSLDCDPAWIREINARAARPDLTLVLEVPAEVAFDRVQRRMAQGAAVEERFDA
                     LALQRRIAEHYRRFRDDPGLGPVRVIDGTTSPTQVTEALIAAVHAVHAVHAVMDAVSL
                     "
     gene            2040..>2436
                     /locus_tag="VGB85_32420"
     CDS             2040..>2436
                     /locus_tag="VGB85_32420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138851.1"
                     /translation="MNHAPTPPPSSTLPLGSSNDPTRGGPAPVPAAPAGPQYIPAQIG
                     VAVQMPGSQPYPQQGYPQQQPYPQPQQYHPQQPGQAHPQQPYPQQQGYPQQPYPQQQQ
                     QPYPQQQGYPQQQAQPPYPPQAQPQHYPQP"
BASE COUNT          320 a          833 c          944 g          339 t
ORIGIN      
        1 atgtcgtcgt cggtgaagtt ccagccgcgg cggttgttgg cgggcgcctt gccggtcgag
       61 acgcgcagct caccgggcac gcgcgcgtcc caggccgcgg ccatcgaggc gctgacctgg
      121 tcgatgagcc aggtccggta gtcgccgggc acgccgcccc acaggcccat gaagtcgggc
      181 gcggagtggc tgtgggtgct gccgatcagg acctgcgcct cgtcgaggcc ggtcagcgcg
      241 gcgaccttgg tccgcacgtc gcgggtgatg cggttgccca tgccgggcag gtcgacgatg
      301 ctcatgatga tcccgccgcc gttgccctcg accgcgaacg agcgcgcgaa gatcgggtcg
      361 tgcacgccct gcgcgggccc gcgggtgtac ggcgccccgt aggcgcccat gtacacgtcc
      421 ttgccggaca ggacctcggg cgagggcgag acgtcctgga cgtgcacgcc gagcaggaag
      481 tgctcgagcg gcatgacacc ggtggtcgtg tcctcgccgg tcgtctcgtc ggtggtcgag
      541 ccggtcgtcg aggtggagcc cgtggcggtg ccggggatgg tgtcgttgcc ggtcgtgccc
      601 gggctcgtgg tcgggctttc ggtcgcaccg ccgtcggcgc tgccggcctc ggtggtgcca
      661 gtgccggtgg tcggcgggtc ggcgctcgtc gtgcccggct cggcggtcga gctgctcgcg
      721 gggtcggtcg taccggtcga gccggcggag ctcgtgccgg tgtcgtcgcc gcatgcggcg
      781 agcagggtga ggagggcgag gcaggcggcg gggactgggc gggacggacg ggtcatcgcg
      841 tcccgaggct atcgcaagcg cgccgggctt ggcgtgcttg cgggcatcac agaggtatga
      901 tctcgcgcgg gggcgccacc ccgcacaggt cagcgctcgt cgtcggtgac gtcgacctcg
      961 tcggcgtcca cgtcggcgtc cacatcgccc tcgtactcgg atagctggta gacgccggtc
     1021 gggtcggggc cgggctgctc ggcgggcagg tcgttggtgt cgtacaccgc ggagtactcg
     1081 acggcctcct cgtcttcctg ggccggggcg gcgatgcggc ggggcgcggc gggtgtcacg
     1141 gcggagggtt gacgcccgga cttccagtcc tcgatggctc tgcacagggt ggcgtagtcg
     1201 atcatgatcg cggccatggt agtccgcgtc cccgcgccgg cgcaaccggc cgcgtgctac
     1261 aggctcacgg cgtccatgac ggcatggacg gcatggacgg catggacggc ggcgatcagg
     1321 gcctcggtga cctgcgtcgg gctggtggtg ccgtcgatca cgcggaccgg gccgagcccg
     1381 ggatcgtccc ggaagcggcg gtagtgctcg gcgatgcggc gctggagggc gagcgcgtcg
     1441 aagcgttcct cgaccgcggc gccctgggcc atgcgtcgct ggacgcgatc gaaggcgacc
     1501 tcggcgggga cctcgagcac gagggtgagg tccgggcggg cggcgcgggc gttgatctcg
     1561 cggatccagg ccggatcgca gtcgagggac tggtaggcga gcgaggacag caggtaacga
     1621 tccgagatca cgaccttgcc ggcggcgacc gccgggatga cctcgcgagt gaggtgatcg
     1681 aggcgatcgg cggcgaagag gagggcgagg gcggccgggt cgagggcggc ggcgcggacg
     1741 ctgagacgct cgcggatgag ggccccgatc gccccggagg tcggctcgcg ggtctccagc
     1801 acctcgcggc cgcgctcgcg aagtcgatcg gcgagcgcgc gcgtttgcag cgtggtgccg
     1861 ctcccgtcga tgccctcgag gacgatgaag cagccccgcg gcgtttcggc catgcggggc
     1921 ggagcgtatc agagcccggc cgccggggac cacccgccgg cacgcggatt gctcgccgac
     1981 cgggccaccc cgtggcacac tcggcgacga cccgggcccg cgcccacagg agattgctcg
     2041 tgaaccacgc cccgaccccg ccgccctcgt ccacgctgcc cctcggcagc agcaacgatc
     2101 ccacgcgggg gggtcctgcg ccagttcctg cggccccggc gggcccccag tacatcccgg
     2161 cgcagatcgg cgtcgcggtg cagatgccgg gctcgcagcc gtatccccag cagggttacc
     2221 cgcagcagca gccgtacccg cagccgcagc agtatcaccc gcagcagccc ggccaggccc
     2281 acccgcagca gccgtatccg cagcagcagg gctatcccca gcagccgtat ccgcagcagc
     2341 agcaacagcc gtatccgcag cagcagggct atccgcagca gcaggcgcag ccgccgtacc
     2401 cgccgcaggc gcagccgcag cactatccac aacctt
//
LOCUS       DASWNJ010001946         2611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_630475, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001946 DASWNJ010000000
VERSION     DASWNJ010001946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630475"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..>2611
                     /locus_tag="VGB85_32425"
     CDS             8..>2611
                     /locus_tag="VGB85_32425"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0138852.1"
                     /translation="MPLSRDSIHSSASAEPTLLATPPGLTPGARRVDPTAPTLTPHAD
                     TRGDANPQRTDDAPAPASIGRFTVLRELGRGGTGVVYAAYDEQLDRKVAVKLLHSETR
                     EDVARVRLMREAQAMARLSHPNIVGVHEVGTFGRQVYVAMEFVHGMTLHAWLKRRRRD
                     FHEIVALFRQAGEGLAAAHDAGLVHRDFKPANVMVGDDGRVRVLDYGLARAEVSPVAA
                     PGLEDLEPAEEHTSALANLRSLVASITLTGRMLGTPAYMAPEQFLGTRVDARSDQFAF
                     CVALFEAAFRSRPFPGDTLAELMASVLAGVVSEPCAPVGVPRALRPLLLRGLQREPAA
                     RWPAMRPLLAALAPMARAPVRRSWFAGAGVAAALAWSGVALATRGAFVDPVCTGGQEQ
                     IEAVWSPEVHARIEQGIDDSGLEQASAVWLRVEDGLDRYSEDWLVGYADACGETGEIL
                     DLRMACLGERHRALATAIRMFEDVDAELLAHADRLIADLPPIDVCGDEKYLRAKVRPP
                     ADPDVKARVDALRKDLQDAQTLESGGQLSEALKALETAIAAAPADYPPLRAELAYTQG
                     TILENLGRYEEARTAFETAFFAAVESGHHEYTIDAANRLAYVIGRRLSDRRTARDWLA
                     HAETYAQREGDPLRRGRVATTAGALLNSAAAMGADTRAEARVAFDQARRAFAEARAED
                     TIDYARYLRFYGDFLASINDPEAAIRAHEASLKWMSDALGKQHPDVAMVHNSLGISLS
                     AAKKHEAALKTLREGLDAAVDLPNQRNHVYMTLYFNSAEAYQELGRLPEAEAAMRAGL
                     AVLERSSGSYLRETHVLQYILAGIIAKAGRPGDAEALLREALARGEATWGPNEPILVD
                     AMATLADLRRQA"
BASE COUNT          348 a         1070 c          865 g          328 t
ORIGIN      
        1 gatcgtcatg cccctgtccc gcgactcgat ccactcctct gcctcggcgg agccgaccct
       61 cctcgccacc ccgcccgggt tgacgccggg ggctcgtcgg gtcgacccca ccgcgccgac
      121 gctgacgccc cacgccgata cgcgcggcga cgccaacccg cagcgcaccg acgacgcccc
      181 cgcccccgcc tcgatcggcc gcttcaccgt gctgcgcgag ctcgggcgcg gcggcaccgg
      241 ggtcgtctac gccgcctacg acgaacagct cgaccgcaag gtcgccgtca agctgctgca
      301 ctccgagacc cgtgaagatg tcgcgcgcgt ccgcctgatg cgcgaggccc aggcgatggc
      361 ccgactctcg caccccaaca tcgtcggcgt gcacgaggtc ggcaccttcg ggcgccaggt
      421 ctacgtcgcc atggagttcg tgcacggcat gaccctgcac gcctggctca agcgccgccg
      481 ccgggacttc cacgagatcg tcgcgctgtt tcgccaggcc ggcgagggcc tggccgccgc
      541 ccacgacgcc ggcctcgtcc accgcgactt caagcccgcc aacgtcatgg tcggcgacga
      601 cggtcgcgtg cgcgtcctcg actacggcct cgcgcgcgcc gaggtctcgc cggtcgccgc
      661 ccctggcctc gaggacctcg agcccgccga ggagcacacc tccgcgctcg ccaacctccg
      721 gtcgctcgtc gcctcgatca cgctgaccgg ccggatgctc ggcacgccgg cgtacatggc
      781 ccccgagcag ttcctcggca cccgcgtcga cgcccgctcc gaccagttcg ccttctgcgt
      841 cgcgctgttc gaggccgcct ttcgcagccg gccattcccc ggcgacaccc tcgccgagct
      901 gatggccagc gtgctcgccg gcgtcgtctc cgagccctgc gcgccggtcg gcgtcccgcg
      961 ggccctgcgt cccctgctgc tgcgcggcct gcagcgcgag cccgccgcgc gctggccggc
     1021 gatgcgtccg ctgctcgccg cgctcgcgcc gatggcccgc gccccggtgc gtcgctcgtg
     1081 gttcgccggg gccggcgtcg cggcggcgct cgcgtggagc ggcgtcgccc tcgcgacccg
     1141 cggcgcgttc gtcgacccgg tgtgcaccgg cggccaggag cagatcgaag ccgtgtggag
     1201 cccggaggtc cacgcgcgga tcgagcaggg catcgacgac tccgggctcg agcaggccag
     1261 cgccgtgtgg ctccgcgtcg aggacggcct cgatcggtac agcgaggact ggctggtcgg
     1321 ctacgccgac gcctgcggcg agaccggcga gatcctcgac ttgcgcatgg cctgcctcgg
     1381 cgagcgccac cgggccctgg cgaccgcgat ccgcatgttc gaggacgtcg acgccgagct
     1441 gctcgcgcac gccgatcgcc tgatcgccga cctcccgccg atcgacgtgt gcggcgacga
     1501 gaagtacctg cgcgccaagg tgcgcccgcc cgccgacccg gacgtcaagg cccgcgtcga
     1561 cgcgctgcgc aaggatctcc aggacgccca gaccctcgag tccggcggtc agctgagcga
     1621 ggccctcaag gccctcgaga ccgcgatcgc ggccgcgccc gccgactacc cgccgctgcg
     1681 cgccgagctg gcctacacgc agggcacgat cctcgagaac ctcggccgct acgaggaggc
     1741 gcgcaccgcc ttcgagaccg cgttcttcgc cgccgtcgag tccgggcacc acgagtacac
     1801 gatcgacgcc gccaaccgcc tcgcctacgt catcggccgc cgcctctccg accgccgcac
     1861 ggcccgcgac tggctcgcgc acgccgagac ctacgcgcag cgcgagggcg acccgctgcg
     1921 ccgcggtcgc gtcgcgacca ccgccggcgc cctcctcaac tcggccgccg ccatgggggc
     1981 cgacacgcgg gccgaggccc gcgtcgcctt cgaccaggcg cgccgcgcct tcgccgaggc
     2041 ccgcgccgag gacacgatcg actacgcccg ttacctgcgc ttttacggcg acttcctggc
     2101 cagcatcaac gacccggagg cggcgatccg ggcccacgag gcgagcctca aatggatgag
     2161 tgacgcgctc ggcaagcagc accccgacgt cgccatggtc cacaacagcc tcggcatctc
     2221 cttgagcgct gcgaagaagc acgaggccgc cctgaagacc ctgcgcgagg gcctcgacgc
     2281 cgcggtcgac ctgcccaacc agcgcaacca cgtctacatg acgctctact tcaacagcgc
     2341 cgaggcctac caggagctcg ggcgcctgcc cgaggccgag gcggcgatgc gcgccggcct
     2401 cgcggtgctc gagcgctcga gcggcagcta cctgcgcgag acccacgttc tgcagtacat
     2461 cctcgccggc atcatcgcca aggctggccg gccgggggac gccgaggccc tgctgcgcga
     2521 ggccctcgcg cgcggcgagg cgacctgggg ccccaacgag ccgatcctcg tcgacgcgat
     2581 ggcgacgctc gccgacctgc ggcgccaggc c
//
LOCUS       DASWNJ010001947         3982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_599897, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001947 DASWNJ010000000
VERSION     DASWNJ010001947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599897"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(394..1860)
                     /locus_tag="VGB85_32430"
     CDS             complement(394..1860)
                     /locus_tag="VGB85_32430"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0138853.1"
                     /translation="MRAPAGARRRSPGSLIWLVARRLLLRERAVASSRAARVWWIFAI
                     ATVVIGGLLVVLGVRSVVPPLAAGVASLALLGATCVRTLTPAVAVAVFGMSLGCAALV
                     TVLGVTSGFEHELTARLARVNGHVLVTEYGQDFDEYPAMVARWRDDPRVVAVSPFAYA
                     MAAVVPAPPEDGAEPAPEEKQLRSPAIAIVKALDPVLAADFPGVPAMLRSGDLQSVLR
                     PGNLRTPPGVALGYRLARRLGVEPGDFVRLVIPAELDGSPESVGRPPRFARFEVLDVV
                     DTGMTEFDTSLALMHISAGQSLFFNEGRVTGVEFALTDPEDAEEFGAEVVAAFGPNFR
                     PTTWRQQSEGTIAGLRQIRAAVSLVLGLLEIVAATALIASLLLLVRRKRAEIASLMAL
                     GADGRAVFWIFEAVGLLAGAVGAAVGVAMGLLLSGLVAAYRYPLSGDVYPVDHLPVHL
                     TVIDVFAPGVAAVLLCALVSGPVALIAAKQPLLPGLQR"
     gene            complement(1865..3661)
                     /locus_tag="VGB85_32435"
     CDS             complement(1865..3661)
                     /locus_tag="VGB85_32435"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0138854.1"
                     /translation="MQTLLLGLFVVSWAALLGPVTLFTDLPRPLGVAAWVGTAALVIL
                     LLRWLLSGALGYESQIGLRIIRTDRVPRGARLAFVACLLVAVGGLLALNLGITSLGLV
                     IAVAAASFLGLLLLLVQYSSIFGTTSIVGVVLGVAALIVVQSVATGFQHEFERRVLGV
                     YAHINVTRSTGMTEYRRFESWLRTQPGVQGASPFVYYAMMLAPFDPEGGRTDDLRLAS
                     VLVKGIEPATAEQVIDLPQHLERGSGRAIPLEVLRSDFELLPVPDREPEELPAAVAAT
                     PDPRGPGWYERAMEHYAAAPAARKRIPGEIRSDLLADDEWPEVEEGVAAGDDARFDAS
                     ALPTIFVGATLAKELGLHEDSLVMVVDPGSSFDSSSEPEFQYYRVVGVFQAGFQEYDS
                     RLVYVHIRELQRFKYRGRDQVSGIDLRLVNPDLAPTVGAAIRGSLPGREYSVLEWQNL
                     NENLFKSIRFQKNIITIILSLVFSVATFNVLSALWTMVVRRSAEVAILMSMGATGGQV
                     ARIFQVTGMAIGLSGSLAGVGFGLILCWLVQLYGYTLDPEVYFIESLPVEISVTQIAW
                     ILGMTLAICFVATLPPSLRAAKLRPVEGLRYE"
BASE COUNT          668 a         1433 c         1402 g          479 t
ORIGIN      
        1 ggctccgtcg cgcccgtgct cggctcggtc gcgcccgtcg tcggcccggg ctcggtggcg
       61 ccagtggcat cggtgtcggt cgcggtgccc tcggagtcgg ggccgcatgc gagggtgagg
      121 ctcagaagcg agagaagaag cgtatgtgga ttggacgaag caaacatgac gcgcctccta
      181 gcaagcgacg tgccgacgca tcacacatcg gcgcccggcg tgttcgccac gatggatgac
      241 agcgatgtca ggcgcgcgcg cagcgatgtc gcgtccgcac cgatccaccc cgaaccaccg
      301 tgaaagcctc ctcgacatgg atcgcgccag cgcgcgagtc gcatgtccgc gaggacaggt
      361 ccacaaaagc ccctgcgatc gcgcccgacg agctcagcgc tgcaagccgg gcagcagcgg
      421 ctgcttggcg gcgatcagcg ccaccggccc gctgaccagc gcgcacagca ggaccgcggc
      481 gacgccgggc gcgaacacat cgatcacggt caggtgcacc ggcaagtggt cgaccgggta
      541 cacgtcgccg ctgagcggat agcgatacgc ggcgaccagc ccgctgagca gcagccccat
      601 cgccaccccg accgcggcgc cgaccgcccc cgcgagcagc ccgaccgcct cgaagatcca
      661 gaacaccgcg cggccgtcgg cgccgagcgc catcaacgac gcgatctcgg cccgcttgcg
      721 ccggaccagc agcagcaagc tggcgatcag cgcggtcgcg gcgacgatct cgagcaaccc
      781 gagcacgagc gagaccgcgg cccggatctg gcgcaggccg gcgatcgtgc cctcgctctg
      841 ctggcgccac gtcgtcgggc gaaagttcgg gccgaaggcc gcgacgacct ccgccccgaa
      901 ttcctcggcg tcctccggat ccgtcaacgc gaactcgacc ccggtgacgc gtccttcatt
      961 gaagaacagc gactgcccgg cgctgatgtg catcagcgcg agcgaggtgt cgaactcggt
     1021 catgccggtg tcgacgacgt cgagcacctc gaacctggcg aacctcggcg ggcggccgac
     1081 cgactcgggt gagccgtcga gctcggcggg gatcaccagg cgcacgaagt caccgggctc
     1141 gacgccgagg cgccgcgcca ggcggtagcc gagcgccacg cccggcggcg tgcgcaggtt
     1201 gccgggacgc agcaccgact ggaggtcgcc ggatcgcagc atcgccggca cgccggggaa
     1261 gtcggccgcg agcaccgggt ccaatgcctt gacgatcgct atcgccgggc tgcgcagttg
     1321 cttttcctcc ggagccggct ccgcgccgtc ctcgggcggg gccgggacca ccgcggccat
     1381 cgcgtaggcg aacggcgaca ccgcgacgac gcggggatcg tcgcgccagc gggcgaccat
     1441 cgccgggtac tcgtcgaagt cctggccgta ctcggtgacg agcacgtggc cgttgacgcg
     1501 ggcgaggcgg gcggtcagct cgtgctcgaa gcccgaggtc acgccgagca ccgtcaccag
     1561 cgccgcgcag ccgaggctca tgccgaacac cgcgaccgcg accgcggggg tcagggtgcg
     1621 cacgcaggtc gcgccgagca gcgcgagcga ggcgacgccg gcggccagcg gaggcaccac
     1681 cgagcggacg ccgagcacca ccagcaggcc gccgatcacg accgtggcga tcgcgaagat
     1741 ccaccacacg cgcgcggccc tgctgctcgc gaccgcacgc tcgcgcagca gcagccggcg
     1801 agcgaccagc cagatcaggc tgcccgggct gcggcgacgg gctccggcgg gcgctcgcac
     1861 gggctcactc gtagcgcagg ccctccaccg ggcgcagctt ggcggcgcgc agcgagggcg
     1921 gcagggtggc gacgaagcag atcgccagcg tcatgccgag gatccacgcg atctgggtga
     1981 cgctgatctc gaccggcagc gactcgatga agtagacctc ggggtcgagg gtgtagccgt
     2041 agagctggac cagccagcac aggatcagcc cgaagccgac ccccgcgagc gatcccgaca
     2101 ggccgatcgc catgccggtg acctggaaga tccgcgcgac ctggccgccg gtcgcgccca
     2161 tcgacatcag gatcgcgacc tccgccgagc gccgcacgac catcgtccac agggccgaca
     2221 gcacgttgaa ggtcgccacc gagaacacca ggctgaggat gatcgtgatg atgttcttct
     2281 ggaagcggat cgacttgaag aggttctcgt tgaggttttg ccactcgagc accgagtact
     2341 cgcggcccgg cagcgagccg cggatcgccg cgccgacggt cggcgcgaga tccgggttga
     2401 ccagccgcag gtcgatgccc gagacctggt cgcggccgcg gtacttgaag cgctgcagct
     2461 cacggatgtg cacgtacacc aggcggctgt cgtactcctg gaacccggcc tggaacacgc
     2521 cgacgacccg gtagtactgg aactcgggct cgctgctgct gtcgaaggag gagcccgggt
     2581 cgacgaccat caccagcgag tcctcgtgca ggccgagctc cttggcgagg gtcgccccga
     2641 cgaagatcgt cggcagggcg ctggcgtcga agcgggcgtc gtcgcccgcc gcgaccccct
     2701 cctcgacctc cggccattcg tcatcggcga gcagatcgct gcggatctcc cccgggatcc
     2761 gcttgcgagc ggccggcgcc gccgcgtaat gctccatcgc ccgctcgtac cagcccggtc
     2821 cgcgggggtc cggggtcgcg gcgaccgcgg ccggcagctc ctccggctcg cggtcgggga
     2881 ccggcagcag ctcgaagtcg ctgcgcaaaa cctcgagggg gatcgctcgc ccgctgccgc
     2941 gctcgaggtg ctgcgggagg tcgatcacct gctccgcggt cgccggctcg atgcccttga
     3001 ccagcacgct ggcgagccgc aggtcgtccg tgcggccgcc ctccgggtcg aagggggcca
     3061 gcatcatcgc gtagtacacg aaggggctcg cgccctggac gccgggctgc gtgcgcagcc
     3121 aggactcgaa gcgccggtat tcggtcatcc cggtgctgcg ggtgacgttg atgtgcgcgt
     3181 agaccccgag cacgcggcgc tcgaactcgt gctggaagcc cgtggcgacc gactgaacga
     3241 cgatcagcgc cgcgacgccg aggacgacgc cgacgatgct cgtcgtcccg aagatcgagg
     3301 agtactggac cagcagcagc agcaggccca aaaagctggc cgccgcgacc gcgatcacca
     3361 gtccgaggct ggtgatcccg aggttgagcg ccaggagccc gccgacggcg accagcaggc
     3421 aggcgacgaa ggccaggcgg gccccgcggg gtacgcggtc ggtgcggatg atccgcaggc
     3481 cgatctggct ctcgtagccg agggcgcccg acagcagcca gcgcagcagc aagatcacca
     3541 gcgcggcggt gccgacccac gcggcgacgc ccaggggccg gggcaggtcc gtgaacagcg
     3601 tcaccgggcc gagcagcgcc gcccacgaga cgacaaagag ccccaggagc agcgtctgca
     3661 cccatcgccg gccgccccgg gtgtgccgat cttgtgcccc tgctaccact gtacggccat
     3721 gaaatttccc ccggagccgg accccgcggg ggctggaagc taacagcccg tccacagcag
     3781 gtcaaactcc gccggacggc tcgccggagc gtggtatgaa tgggcccggt ggacccgcaa
     3841 cgacacggga gatcgcggcc tcgacgatcc ctcgcgaatc accctcggat cccccggatc
     3901 gggcgttatc gccggcccag agggcccaag cgcgaacctt gacacgcctc gctggcgcaa
     3961 gttacgctgc gccgaccgct ga
//
LOCUS       DASWNJ010001948         1725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_282098, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001948 DASWNJ010000000
VERSION     DASWNJ010001948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282098"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /gene="sctW"
                     /locus_tag="VGB85_32440"
     CDS             <1..785
                     /gene="sctW"
                     /locus_tag="VGB85_32440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA];
                     GO_process: GO:0050709 - negative regulation of protein
                     secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     SctW"
                     /protein_id="HEY0138855.1"
                     /translation="ESPLSMLQNAAEELTFGAHERMSRRDLGERRLERKDPSRQLEQV
                     ERIQEYLDNLPDFDRGKVDQMLERLLGRGEGGQGQPSSSSRDGLGEALRGFHEDVTYQ
                     QAALEVMDQALAGRPDPQSQALRAEVQAKIAANARELAPAIQAGLNVTAQAMLAGGDA
                     KEVQELRNLYRETVLSHESLGKSWHNIVERYGEGDLGGKITFLLKAIGADLASRGPSI
                     APAELKAILDETHQLETLSTIRERAAGFLKRLHARFGAEAGG"
     gene            782..1099
                     /locus_tag="VGB85_32445"
     CDS             782..1099
                     /locus_tag="VGB85_32445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TyeA family type III secretion system gatekeeper
                     subunit"
                     /protein_id="HEY0138856.1"
                     /translation="MSGAAAKGAMAAADRLMGSLLGLAEASWISGAQVEKLAREQRLG
                     STEAEIYFLRELADLVRAMPLKAFKDPEQRLKLVDAVQETLDKAIEREEAELDEEGDD
                     APP"
     gene            1096..1488
                     /locus_tag="VGB85_32450"
     CDS             1096..1488
                     /locus_tag="VGB85_32450"
                     /inference="COORDINATES: protein motif:HMM:NF044537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138857.1"
                     /translation="MSWVDDAVAAFGRDMGMAGLRLPADGAVELAFERRGTLALERSG
                     GDGDGRVGDDLLVYLTRARPHGGPERLAKALALCHWRHDRAPPVQAGAKDDHLVLLFR
                     LRAREVTPEAIAQGFDRLVRLMDELEQA"
     gene            1527..>1725
                     /locus_tag="VGB85_32455"
     CDS             1527..>1725
                     /locus_tag="VGB85_32455"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0138858.1"
                     /translation="MDLATQEREGDVLVVVARGDLNAGSCGRLEQELNARIDAGRRRL
                     VLDLGEIRYVSSAGLRVFLLAA"
BASE COUNT          255 a          603 c          659 g          208 t
ORIGIN      
        1 aggagagccc gctctccatg ctccagaacg ccgcggagga gctcaccttc ggcgcgcacg
       61 agcggatgtc gcggcgcgac ctcggcgagc gtcgcctgga gcgcaaggat ccctcgcgcc
      121 agctcgagca ggtggagcgg atccaggagt acctcgacaa cctgccggac ttcgaccgcg
      181 gcaaggtcga ccagatgctg gagcggctcc tcggccgtgg cgagggcggc caaggccagc
      241 cgtcgtcgtc gtcgcgcgac gggctgggcg aggcgctccg gggcttccac gaggacgtca
      301 cctaccagca ggcggcgctc gaggtgatgg accaggcgct ggcaggccgg cccgacccgc
      361 agtcgcaggc gctgcgcgcc gaggtgcagg ccaagatcgc cgcgaacgcg cgcgagctgg
      421 cccccgcgat ccaggccggg ctcaacgtta ccgcccaggc gatgctcgcc ggcggcgacg
      481 ccaaggaggt ccaggagctc cggaacctct accgggagac cgtcctctcc cacgagtccc
      541 tgggcaagag ctggcacaac atcgtcgagc gctacggcga gggcgacctc ggcggcaaga
      601 tcactttcct gctcaaggcc atcggcgccg acctcgcctc gcgcggcccg tcgatcgccc
      661 cggccgagct gaaggcgatc ctcgacgaga cgcaccagct cgagaccctg tcgacgatcc
      721 gagagcgcgc ggcgggcttc ctcaagcgcc tgcacgcgcg gttcggcgcg gaggcgggcg
      781 gttgagcggc gccgcagcca agggcgcgat ggccgcggcc gaccggctga tgggctcgct
      841 gctgggcctc gccgaggcga gctggatctc cggcgcccag gtcgagaagc tcgcgcgcga
      901 gcagcggcta ggctcgacgg aggccgagat ctacttcctg cgcgagctgg cggacctggt
      961 ccgcgccatg ccgctcaagg cgttcaagga ccccgagcag cgcctcaagc tcgtggacgc
     1021 ggtccaggag acgctcgaca aggcgatcga gcgcgaggag gcggagctcg acgaggaggg
     1081 ggacgacgcg ccgccatgag ctgggtcgac gacgcggtcg cggccttcgg gcgcgacatg
     1141 ggcatggccg ggctgcgctt gcccgccgat ggcgcggtcg agctcgcctt cgagcggcgg
     1201 ggcacgctcg ccctggagcg ctccggcggc gacggggacg gtcgcgtcgg cgacgacctg
     1261 ctcgtctacc tgacgcgcgc ccggccgcac ggcgggcccg agcgcctcgc caaggcgctc
     1321 gcgttgtgcc attggcgaca tgatcgcgcg ccgcccgtgc aggccggcgc gaaggacgac
     1381 cacctcgtcc tcctgttccg gctgagggcc cgggaggtga cgccggaggc gatcgcgcaa
     1441 ggcttcgacc ggctggtccg gctcatggac gaactcgagc aggcctgacc gggcgggcgg
     1501 cgaaccccgg gggaaggacg gcgaacatgg atctcgcgac ccaggagagg gagggcgacg
     1561 tgctggtcgt ggtggcgcgt ggcgacctga acgcgggcag ctgcggcagg ctcgagcagg
     1621 agctcaacgc gcggatcgac gccggccggc gccggctcgt gctcgacctg ggcgagatcc
     1681 gctacgtctc ctccgcgggg ctgcgcgtgt tcctgctggc ggcgc
//
LOCUS       DASWNJ010001949         2403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_457098, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001949 DASWNJ010000000
VERSION     DASWNJ010001949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457098"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VGB85_32460"
     CDS             <1..638
                     /locus_tag="VGB85_32460"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY0138859.1"
                     /translation="ANRVAALLDAGAEIDAKNTGAGGIPTPLIAAAFCGHTEVVRLLL
                     ARGADVDAVNSQGRNALVQAADQGHAEIVELLAHAGSAVDQPGLFGQTALHLAAWQGH
                     RAVVETLLACAARTDLRDDAGMTPLALACTEDVPEVVELLLAAGAPVEERSGSTDATP
                     LIMAAIDGQARVAGVLLHHGACRDARDRNGRTAAEWARAEGHESVAQLLEA"
     gene            complement(642..1709)
                     /locus_tag="VGB85_32465"
     CDS             complement(642..1709)
                     /locus_tag="VGB85_32465"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0138860.1"
                     /translation="MKLPRSLALILVLSACAGSTRKMDPADGALGNLPGRWENAFVEI
                     DTSTGPLRMHYVAAGPKDAPRVILLHGFPDFAYTWHDVVPMLSGEYRVLAPDMRGYAA
                     TGKPRKGYDLDTLGADIIKFADATAAVDGAPADRPTHLLGHDWGAAVGWWAVLTAPQR
                     FTTYTAISVAHPRAFTEFLKKSEEQRKKSRYMRKLTLPLVPVFFAGMSDKRRRGVYRD
                     ELARKDAFGDNELVWYRAAFDSTMETRPPLRYYKQRFRNADENEQFARRAPAISIPVL
                     VLWGKKDKYLMSEMAPDSCKYVLPNLCQVEVFPEATHWVHWDDPKAVVTRWQKFIGEL
                     APADKQPDAGATTPATPAGPA"
BASE COUNT          317 a          861 c          879 g          346 t
ORIGIN      
        1 atgcgaaccg cgtcgcggcg ctgctcgacg cgggcgccga gatcgacgcg aagaacaccg
       61 gcgccggagg gatccccacg ccgctgatcg cggcggcgtt ttgcggccac accgaggtcg
      121 tgcgcctgct gctggcccgc ggcgccgacg tcgacgcggt caactcgcag ggccgcaacg
      181 cgctggtcca ggccgccgac cagggccacg cggagatcgt cgagctgctc gcccacgcgg
      241 ggtccgccgt cgaccagccg ggcctgttcg ggcagaccgc cctgcacctc gcggcctggc
      301 agggccaccg cgcggtcgtc gagacgctgc tcgcctgcgc cgcgcgcacg gacctgcgcg
      361 acgacgccgg catgacgccg ctcgcgctcg cgtgcacgga ggacgtgccc gaggtcgtcg
      421 agctgctgct cgccgccggc gcgccggtcg aggagcgcag cgggtcgacc gacgcgacgc
      481 ccctcatcat ggccgcgatc gacggccagg ctcgcgtcgc cggcgtgctg ctgcaccacg
      541 gcgcgtgccg tgacgcccgt gaccgcaacg gacgcacggc tgccgagtgg gcccgcgccg
      601 agggccacga gtcggtcgcg cagctgctcg aggcgtgaag cttacgcggg gccagccggc
      661 gtcgcggggg tcgtcgcgcc agcgtccggt tgcttgtcgg cgggcgcgag ctcgccgatg
      721 aacttctgcc agcgggtgac gacggccttg gggtcgtccc agtgcaccca gtgggtcgcc
      781 tcggggaaca cctcgacctg gcacagattc ggcagcacgt acttgcagct gtcgggcgcc
      841 atctccgaca tcaggtactt gtctttcttg ccccacaaca cgagcacggg gatcgagatc
      901 gcaggggccc ggcgggcgaa ctgctcgttc tcgtcggcgt tgcggaacct ctgcttgtag
      961 tagcgcagcg gcgggcgcgt ctccatcgtc gagtcgaagg ccgcccggta ccacaccagc
     1021 tcattgtccc cgaacgcgtc cttgcgcgcg agctcgtcgc ggtacacccc gcgccgccgc
     1081 ttgtcggaca tgcccgcgaa gaacaccggc acgagcggca aggtcagctt gcgcatgtac
     1141 ctgctcttct tgcgctgctc ctccgacttt ttaagaaact cggtgaacgc ccgcggatgc
     1201 gcgacgctga tcgccgtgta cgtggtgaag cgctgcgggg cggtcagcac ggcccaccag
     1261 ccgaccgccg cgccccagtc gtgccccagc aagtgcgtcg gccgatcggc cggcgcgccg
     1321 tcgacggccg cggtcgcgtc ggcgaacttg atgatgtccg ccccgagcgt gtcgaggtca
     1381 taacccttgc gcggcttgcc ggtcgccgca tacccgcgca tgtccggcgc gagcacgcga
     1441 tactcgcccg acagcatcgg cacgacgtcg tgccaggtgt acgcgaagtc cgggaagccg
     1501 tgcaggagga tgacccgcgg cgcgtccttg gggcccgcgg cgacataatg catgcgcagc
     1561 ggcccggtcg aggtgtcgat ctcgacgaag gcgttctccc agcgcccggg caggttgccg
     1621 agcgcaccgt cggcggggtc catcttgcgc gtcgacccgg cgcaggccga caacacgagg
     1681 atcagcgcga ggctccgggg cagcttcatc gccgggcttg taccacgggg cgcgatgccc
     1741 cccaagaaga ccggctcgac caggcccccc ggcgtgcggt cggggcgcgg tgtccggccg
     1801 agctcggggc tcggggtggt cgggctgcgg gggctgcggt tgctcgggct gcggtggctc
     1861 gggctgccgt ggctcgggct gccgagtcgc tcgggctgcc gagtcgctcg gggacgcttg
     1921 gagtgctcgg gcagccggtg gcgagcccgg gaaacgcggg gcgagacttc atgggccggg
     1981 gggccgactg gctgcggggc cctgcgacgc gggtcctccc agggcacgtg ctcgccgccc
     2041 cacctgtccg gaagaacatg tcactttcac catcacttcc gtcgccgctc gtgactggcg
     2101 gtggggcgtc tgcgcgcgag ccctgggtcc tcccgctgct tcccccgccg gtcggcccca
     2161 ccgggagcac accgtctgcg ccccgcttgt cggtgctcgc ctatcatcga gaaaccggac
     2221 gctgcggcgg tggtcgtcgc acgcgccggt gaggtctcca gtcgagcgac acggcgcatg
     2281 cctgtgacga gcggggcgca gacgatgtcc gcccggggag caggtgccga ctggcggggg
     2341 aagcagcggg aggacccagg ggtcacgcgc agacgccccg tcaacctccg ttcatcgcga
     2401 tgc
//
LOCUS       DASWNJ010001950         2582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_528531, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001950 DASWNJ010000000
VERSION     DASWNJ010001950.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528531"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..1986)
                     /locus_tag="VGB85_32470"
     CDS             complement(103..1986)
                     /locus_tag="VGB85_32470"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 family protein"
                     /protein_id="HEY0138861.1"
                     /translation="MTEPRKPAEDDSDELAEHFPVAASSLMAMRLTPEMRRVMQATQT
                     KSSAKASSKATMDGLPRPMPQYKLSLRTWSIPVLAILGLAVIIFVVAFWLNIITDPVD
                     SGNEVSAHGLLQAILFQDVEQARNTLNGLGEVMAAVLGLALTVSSIIVQLAATRFTPH
                     ITSLFFRARTNLLVLGFFVTATVFVVWVNFSIGATYVPRWGVLFSMLLMTASLLLLFP
                     YFAYVFDFLDPEKIVGRITANGLYACTPIRGKAAQAVDRRQLQSIEAVEHLTNIGLNA
                     LQQKDKNIASNAIDALCNFAVLYGETKAGMLKDWHRVSQWNRQSPDFVSLSTEAILDL
                     SERGTWLEWKILRQYQMIFGEGLTQIKDVCYLIAIDTRRLGEAAARRGDLHALDLAIK
                     FFNTYLRATFNANDIRTAYNTLHQYRQLGEMILAEGRNISRTRPSRSEREQLEARERA
                     DILERRAVDIARYMRYYSGIAFQRGMIFIAEVIAHDIGTLCAEAHRAHSACHQKLLDY
                     FLAVDDVAESAEQQKALSGVRLAQIKLATNYLLHGAVHFAKQIADDMAGEDHRRLRGM
                     WQRLVRLDIREFWEVNDRGVNFEYLPPEQKALLPRFFAWFEGLRFEQPEDEAMNNSVR
                     LHP"
     gene            2152..>2582
                     /locus_tag="VGB85_32475"
     CDS             2152..>2582
                     /locus_tag="VGB85_32475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138862.1"
                     /translation="MLQHAGLDDALLGPRLVRLLAPDSPLPAREMVARGLAPLAGAEP
                     WLALYQLWVRGGPTLAELASRTAGELPAAVVMTALADPHLPPVVLDFMARRRIRGPET
                     LRLVVHHPRVHDSTLGVLARAAPEMVCSAIAKNQRRWLACPA"
BASE COUNT          380 a          833 c          950 g          419 t
ORIGIN      
        1 agctgcgcgt agaacctggc cgatcggtca gggtcggtga cgcgcagagc aatgtggtgc
       61 agtccgaggt ggtgcagtcc gaggtggtgc agtcccatct gctcacgggt gcaggcgcac
      121 cgagttgttc atcgcctcgt cctcgggctg ctcgaagcgc aggccctcga accacgcgaa
      181 gaagcgcggc agcagggcct tttgttcggg cggcaggtac tcgaagttga cgccgcggtc
      241 gttgacctcc cagaactcgc ggatgtcgag gcgaacgagc cgctgccaca tgccacgcag
      301 acggcggtgg tcctcgccgg ccatgtcgtc ggcgatctgc ttggcgaagt ggaccgcgcc
      361 gtgcaggagg tagttggtgg cgagcttgat ctgcgccagg cgcacgccgc tgagcgcctt
      421 ttgctgctcg gcggactcgg cgacgtcgtc gacggcgagg aagtagtcga gcagcttctg
      481 gtggcacgcg ctgtgggctc gatgggcctc ggcgcacagc gtgccgatgt cgtgggcgat
      541 cacctcggcg atgaagatca tgccgcgctg gaacgcgatg ccgctgtagt agcgcatgta
      601 gcgggcgata tcgacggcgc ggcgctcgag gatgtcggcg cgctcgcggg cctccagctg
      661 ctcgcgctcg gagcgggacg gcctggtgcg gctgatgttg cgcccctcgg cgaggatcat
      721 ctcgccgagc tggcgatact ggtggagggt gttgtacgcg gtgcggatgt cgttggcgtt
      781 gaaggtggcg cgcaggtagg tgttgaagaa cttgatcgcg aggtcgaggg cgtggaggtc
      841 gccgcgacgc gccgcggcct cgccgagccg gcgggtgtcg atcgcgatca ggtagcagac
      901 gtctttgatc tgggtcaggc cctcgccgaa gatcatctgg tactggcgga ggatcttcca
      961 ctcgagccag gtgccgcgct cgctgaggtc gaggatcgcc tcggtcgaga gcgacacgaa
     1021 gtcggggctc tggcggttcc actgcgagac ccggtgccag tccttgagca tgccggcctt
     1081 ggtctcgccg tacaggaccg cgaagttgca cagcgcgtcg atcgcgttcg aggcgatgtt
     1141 cttgtccttt tgttgcagcg cgttgaggcc gatgttggtc aggtgctcga ccgcctcgat
     1201 cgactgcagc tggcgccggt cgaccgcctg cgcggccttg cctcggatag gagtgcacgc
     1261 gtagagacca ttggcggtga tgcgcccgac gatcttctcc gggtcgagga agtcgaacac
     1321 gtaggcgaag tacgggaaca gcagcagcaa gctggccgtc atcagcaaca tcgagaacag
     1381 cacgccccag cgcgggacgt aggtcgcgcc gatcgagaag ttgacccaca cgacgaacac
     1441 cgtcgccgtg acgaagaagc cgagcaccag caggttggtg cgcgcgcgga agaacagcga
     1501 ggtgatgtgc ggcgtgaagc gcgtcgcggc cagctgcacg atgatgctgg agaccgtcag
     1561 cgcgaggccg agcaccgcag ccatgacctc gccgaggccg ttgagggtgt tgcgcgcctg
     1621 ctcgacgtcc tggaacagga tcgcctgcag cagcccgtgc gcgctgacct cgttgccgct
     1681 gtcgaccggg tcggtgatga tgttgagcca gaaggcgacg acgaagatga tcaccgcgag
     1741 gccgaggatc gccagcaccg ggatcgacca ggtgcgcagc gacagcttgt actgcggcat
     1801 cggccgcggc aggccgtcca tcgtcgcctt gctcgaggcc ttcgccgacg acttggtctg
     1861 cgtcgcctgc atcacacgcc gcatctccgg cgtcaggcgc atcgccatca gcgagctcgc
     1921 cgcgaccggg aagtgctccg cgagctcgtc gctgtcgtcc tcggcgggct tcctcggctc
     1981 ggtcacggcg ctgacggtaa catgaggacc cggccgcgcg ggacccggga ggaccgccgc
     2041 agcgccgcgt cgcgccccga ggcgtcgcgc gcagcggcgt cgatctcggc ggcgcccggt
     2101 cgatctcgac gccgggcgat cggcgcgcgg atccgtggta gtactcgtgc ggtgctgcag
     2161 cacgccggac tcgacgacgc cctgctcggg ccccggctgg tgcgcctgct ggcgcccgac
     2221 tccccgttgc ctgcccgcga gatggtcgcc cgcggcctcg cgccgctcgc gggcgccgag
     2281 ccgtggctgg ccctgtatca gctgtgggtg cgaggcggac cgacgctcgc ggagctggcc
     2341 agtcgcacgg ccggcgagct gccggccgcc gtcgtgatga ccgcgctcgc cgacccgcac
     2401 ctgccgccgg tggtgctgga cttcatggcc cggcgacgga tccgcggacc ggagacgctg
     2461 cgcctcgtcg tgcatcaccc gcgcgtgcac gacagcacgc tcggcgtgct cgcccgcgcc
     2521 gcgccggaga tggtgtgcag cgcgatcgcc aagaaccagc ggcgctggct ggcgtgcccg
     2581 gc
//
LOCUS       DASWNJ010001951         2167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_457117, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001951 DASWNJ010000000
VERSION     DASWNJ010001951.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457117"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VGB85_32480"
     CDS             <1..439
                     /locus_tag="VGB85_32480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138863.1"
                     /translation="WNAYAHGAGDLTTANVDELRASLDVSLHGMLVATQAALPDLRSE
                     KGALLVTGGGLAFYDAKVDAMAVSWGAMGLAVAKAAQHKAVGLLHARLAGEGVYVGEV
                     VVLGSVKGTAFDGGSATLEASAIAERFWGLFQARSEASVTITG"
     gene            complement(458..>2167)
                     /gene="acs"
                     /locus_tag="VGB85_32485"
     CDS             complement(458..>2167)
                     /gene="acs"
                     /locus_tag="VGB85_32485"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEY0138864.1"
                     /translation="HLDGPARDRPAIVWEGEPLGADGQPETRTLTYAQLHTEVVRLAG
                     ALAALGVGLGDRVIIYMGMVPEAVIAMLACARLGAVHSVVFGGFAADALHSRIIDCQA
                     RVVLTQDGAWRRGAVIAMKPVVDAAVKLAQCVEKVVVLRRVADALPVTMTPGRDLWWD
                     EALANAHTSETAPVQVDPEHPLFILYTSGSTGKPKGVLHSSAGYLAGVYLSARYVFDL
                     QPDDLFWCTADIGWVTGHSYVVYGPLANAATILIYEGAPNAPDPGRFWQIIERHRVTI
                     LYTAPTAIRAFMRWGDEWPRQRDLSSLRLLGSVGEPIGPEAWSWYHQTIGGGRCPVVD
                     TWWQTETGAIMLTTLPGAVAAKPGSTGLPFFGVEPAVVHADGSPCGPEENGLLVLRTA
                     WPSMLRTIWGDDERFRQTYFTTIPGVYFAGDSARIDRNGYITVLGRVDDVLNVAGHRI
                     GTAEIEAAIGTHPAVAESAAVGRPDDLKGQALVVFVTLRMGQEATPALKDEIRDRVSQ
                     EVGKFARPDEIRFTDALPKTRSGKIMRRFLKDIAAGKATSGDTSTLEDHGVLTRLQAS
                     DDE"
BASE COUNT          304 a          726 c          824 g          313 t
ORIGIN      
        1 ctggaacgcg tacgcccacg gcgcgggtga cctgaccacc gcgaacgtcg acgagctgcg
       61 cgcctcgctc gacgtgtcgc tgcacggcat gctggttgcg acccaggcgg cgctgcccga
      121 cctgcgcagc gagaagggcg cgctgctggt caccggcggc gggctcgcgt tttatgacgc
      181 gaaggtcgac gcgatggcgg tgtcgtgggg cgcgatgggc ctcgcggtgg cgaaggcggc
      241 gcagcacaag gccgtcggcc tgctgcacgc ccggctcgcg ggcgagggcg tgtacgtcgg
      301 cgaggtcgtg gtgctcggca gcgtcaaggg caccgcgttc gacggcggca gcgcgacgct
      361 cgaggcgtcg gcgatcgcgg agcggttctg ggggctgttt caggcccgca gcgaggcgtc
      421 ggtcacgatc acgggttgac cgccgccgcg gtcgcgctca ctcgtcgtcg ctcgcttgca
      481 ggcgggtgag cacgccgtgg tcctcgaggg tgctggtgtc gccgctggtc gccttgccgg
      541 cggcgatatc ctttaaaaag cggcgcatga tcttgccgct gcgggtcttg ggcagggcgt
      601 cggtgaagcg gatctcgtcg ggccgggcga acttgccgac ctcctgggac acgcgatcgc
      661 ggatctcgtc cttgagcgcc ggggtggcct cctggcccat gcgcagggtg acgaacacga
      721 cgagcgcctg gcccttgagg tcgtcggggc gcccgaccgc ggccgactcg gcgacggccg
      781 ggtgagtgcc gatcgcggcc tcgatctcgg cggtgccgat ccggtggccg gcgacgttga
      841 gcacgtcgtc gacgcgaccg agcacggtga tgtaaccatt gcgatcgatg cgcgcgctgt
      901 cgcccgcgaa gtacacgccg gggatcgtgg tgaagtaggt ctgccgaaag cgctcgtcgt
      961 cgccccagat cgtgcgcagc atcgagggcc acgcggtacg cagcaccagc aggccgttct
     1021 cctcggggcc gcaggggctg ccgtcggcgt gcacgacggc cggctcgacg ccgaagaagg
     1081 gcaggccggt gctgccgggc ttggccgcga cggcgccggg cagggtggtc agcatgatcg
     1141 cgccggtctc ggtctgccac cacgtgtcga cgaccgggca ccgcccgccg ccgatcgtct
     1201 gatgatacca gctccacgcc tccggcccga tcggctcgcc gacggagccg agcaggcgca
     1261 ggctcgagag gtcgcgctgg cgcggccact cgtcgcccca gcgcatgaag gcgcggatcg
     1321 ccgtcggcgc ggtgtagagg atcgtgacgc ggtggcgctc gatgatctgc cagaaccgcc
     1381 ccggatcggg cgcgttgggg gcaccctcgt agatcaggat cgtcgcggca ttggcgagcg
     1441 ggccgtatac gacgtagctg tggccggtga cccagccgat gtcggcggtg caccagaaca
     1501 ggtcgtcggg ctggaggtcg aagacgtagc gggccgacag atatacgccc gcgaggtatc
     1561 ccgccgagct gtgcagcacg cccttgggct tgccggtcga cccggaggtg tagagaatga
     1621 acagcgggtg ctcggggtcg acctgtaccg gtgcagtctc cgaagtgtgt gcattcgcca
     1681 gggcctcgtc ccaccacagg tcgcgcccgg gggtcatcgt gacgggcagg gcgtcggcga
     1741 cgcggcgcag cacgacgacc ttctcgacgc actgcgcgag cttgacggcg gcgtcgacga
     1801 cgggcttcat ggcgatcacg gcgccgcggc gccacgcgcc gtcctgggtg agcacgaccc
     1861 gcgcctggca gtcgatgatc ctgctgtgca gggcgtcggc cgcgaacccg ccgaacacca
     1921 ccgaatgcac ggcgccgagc cgcgcgcacg ccagcatggc gatcacggcc tcgggcacca
     1981 tgcccatgta aatgatgacg cgatcgccga ggccgacgcc gagggccgcg agcgcgcccg
     2041 cgagccgcac gacctcggtg tgcagctgcg cgtaggtcag ggtccgcgtc tcgggctgac
     2101 catcagcgcc gagcggctca ccctcccaca cgatcgcggg ccggtcgcgc gcgggaccgt
     2161 cgaggtg
//
LOCUS       DASWNJ010001952         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_417084, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001952 DASWNJ010000000
VERSION     DASWNJ010001952.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417084"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VGB85_32490"
     CDS             <1..361
                     /locus_tag="VGB85_32490"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEY0138865.1"
                     /translation="VDGRSVVGPRARPDADARSAVAIRRDGTVLFVLLFAEQAVERAA
                     PGRVELGARSSSTGLSLAAWADLLARPESDGGLGVDVALNLDGGYSTSASIHVGAFDL
                     EVLAHGATINALRACAE"
     gene            604..1221
                     /locus_tag="VGB85_32495"
     CDS             604..1221
                     /locus_tag="VGB85_32495"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0138866.1"
                     /translation="MEVSIEAARVEGLLDVIVAASQGDYEGRVTLRENDDAFLEVEVG
                     VNYMLEELAGQRDRSARQHDELLANERRLAQQQAELVQALSTPILEVWPGVLALPLIG
                     RIDDDRAATITTTLLDRVAASRASRVILDLTGVGALDPGSMSALLRMVRAIELLGARC
                     LWTGLGPDVACQIVALDAGPTPIRTFARLADALALVLTPRGASRR"
     gene            1231..1641
                     /locus_tag="VGB85_32500"
     CDS             1231..1641
                     /locus_tag="VGB85_32500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138867.1"
                     /translation="MPLRFEPGHTPHGHRCLRIHAWGLISLADAELLLARLGPGGDHH
                     RERILAFPDGSLSYAPEARKLLPMLKREYLAIGIVLHSAIVRAGINLMVRIAGDISPL
                     RMFAGEDEALTWLDTLEVTANATPAIRAAPAAAG"
     gene            complement(1645..2220)
                     /locus_tag="VGB85_32505"
     CDS             complement(1645..2220)
                     /locus_tag="VGB85_32505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138868.1"
                     /translation="MTLPILAVRAETRAELGSFDAAFASLNKYFDVVEAAEDADIAAA
                     DAVIEALDGLEHSSDIDSAYFACTLTAPELGSDEFFAATGIGRDERQSVGGYDNPKLA
                     VITAGDGEDERPPVLLITWLNNIELEDAAEYVGDEGVVAAEQAAAVLARSGKIQGVQI
                     CTDDRATKFQMVVAKQRSGVYAGVLTVSVET"
BASE COUNT          332 a          806 c          780 g          346 t
ORIGIN      
        1 cgtcgacggg cgcagcgtgg tcggtccacg cgcgcgcccg gacgccgatg cccgcagcgc
       61 cgtggcgatc cgccgcgatg gcacggtgct gttcgtgctg ttgttcgccg agcaggcggt
      121 cgagcgcgcg gcccccgggc gggtcgagct cggggcgagg agcagcagca ccggcctgag
      181 cctcgccgcc tgggccgacc tgctcgcgcg tcccgagtcc gacggtggcc tcggcgtcga
      241 cgtggcgctc aacctcgacg gcggctactc gacctcggcc tcgatccacg tcggcgcgtt
      301 cgacctcgag gtgctcgccc acggcgcgac gatcaacgcc ctgcgcgcct gcgccgaata
      361 acgcgggcag atcctcgcaa tctggcgtca ttcgtgtcca tgtcacgacg atatatccac
      421 acttcgaatc gaccgccgaa catcgcacga aattaggacg cgagcgacaa aaccgcgcca
      481 ggcgcccgtg gcgctcggca catttaccat atcgtcgaga aacgagagat cacgcagatc
      541 cgttcacggc atgcgcccgg aggcgtgctt gacgggcatc acgaccactt ttatgttgcg
      601 atcgtggagg tctcgatcga agcggcccgc gtcgagggcc tgttggacgt catcgtggcg
      661 gcgtcccagg gagattacga ggggcgcgtg acgctgcgag agaacgacga cgcattcctc
      721 gaggtcgagg tcggcgtcaa ttatatgctc gaggagctcg ccggccagcg cgaccgcagc
      781 gcgcggcagc acgacgagct gctggcgaac gagcgcaggc tcgcgcagca gcaggcggag
      841 ctggtccagg cgctgtcgac cccgatcctc gaagtatggc ccggcgtgct cgcgctcccg
      901 ctgatcgggc gcatcgacga cgaccgcgcc gccacgatca ccaccaccct gctcgaccgc
      961 gtcgcggcgt cgcgagcgag ccgcgtgatc ctcgatctga ccggcgtggg tgccctggac
     1021 cccggcagca tgtcggcgct gctgcgcatg gtccgggcga tcgagctgct cggtgcgcga
     1081 tgtctatgga ccggcctcgg gcccgacgtc gcctgtcaga tcgtcgcgct cgacgccggg
     1141 ccgacgccga tccgcacgtt cgcgcgcctc gccgacgcgc tggccctcgt gctcactcct
     1201 cgcggcgcct cgcgcagata ggaccgaccg atgccattac gttttgaacc tggacatacg
     1261 ccgcacgggc accggtgcct gcggatccac gcctggggcc tgatctcgct cgcggacgcc
     1321 gagctgctgc tcgcccggct cggaccggga ggcgaccacc accgcgagcg catcctcgcg
     1381 ttcccggacg gcagcctgag ctatgcaccg gaggcccgca agttgctgcc gatgctcaag
     1441 cgggagtacc tggcgatcgg gatcgtgctg cacagcgcga tcgtccgcgc cggcatcaac
     1501 ctgatggtcc gcatcgccgg cgatatctcg ccgctgcgta tgttcgccgg agaggacgag
     1561 gcgctgacct ggctcgacac tctcgaggtc accgcaaacg cgaccccggc gatccgggcg
     1621 gcccccgccg cggccggttg acgctcaggt ctcgacgctg accgtcagga cgccggcgta
     1681 cacgccgctg cgttgcttcg cgacgaccat ctggaacttg gtcgcccggt cgtcggtgca
     1741 gatctggacg ccctggatct tgcccgacct ggcgagcacg gccgccgcct gctcggcggc
     1801 gacgaccccc tcgtcgccga cgtactcggc ggcgtcctcg agctcgatgt tgttgagcca
     1861 cgtgatcagc aggaccggcg ggcgctcgtc ctcgccgtcg cccgccgtga tgaccgcgag
     1921 cttggggttg tcgtagccgc cgacgctctg ccgctcgtcg cggccgatgc cggtcgccgc
     1981 gaagaactcg tcggacccga gctccggggc cgtcaacgtg caggcgaagt aggcgctgtc
     2041 gatgtcgctc gagtgctcga ggccgtcgag cgcctcgatc accgcgtccg cggccgcgat
     2101 gtcggcgtcc tcggccgcct cgacgacgtc gaagtacttg ttgagagagg cgaacgcggc
     2161 gtcgaaggag ccgagctccg cgcgggtctc ggcgcgaacg gcgaggatgg ggagggtcat
     2221 gggccggcgt ttaccacaaa cggcgccgac acggcgccgc ccgc
//
LOCUS       DASWNJ010001953         2845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_105894, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001953 DASWNJ010000000
VERSION     DASWNJ010001953.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105894"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VGB85_32510"
     CDS             <1..490
                     /locus_tag="VGB85_32510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138869.1"
                     /translation="RFARAVLDHREARTAIYPVGASGVGSDAEAELRLAAKATGGQYI
                     FLTDHSGVGGHHEAAKVEQYKVETLHDAMTRMIRGELGDAKVQARTAPLETCDETPMP
                     MPTPVALDASALVPVEAPAVSPWVQLRERLEGNLLFALGMLVMVLAGIGFDTLIHRWR
                     TR"
     gene            complement(558..1145)
                     /locus_tag="VGB85_32515"
     CDS             complement(558..1145)
                     /locus_tag="VGB85_32515"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0138870.1"
                     /translation="MERRLTAIHRVFAASLVCAAGLFIASAVNGKNGAQDEVGKRLSD
                     RAEIAELAYCYAEGTDAIGGGDLAAGKAVYGDCFTAGATVKAWFPGADFNGPPGLSAT
                     GTDAWADAVASVFTGNGYIATQHAMSNIRISLDGNSGTMRSYLSATHVVDPDGAVDLA
                     HGTYVDTVVRTPQGWRISARSLYLIDFLRLESPTP"
     gene            complement(1219..2097)
                     /locus_tag="VGB85_32520"
     CDS             complement(1219..2097)
                     /locus_tag="VGB85_32520"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY0138871.1"
                     /translation="MRPTMYLESPATTTASSTPAADWFVTASEVFKLYAIPFLWQVLG
                     AIGIWIVGGILIRFACRMVGTAMTARNLDRTLTSYVTSALSILLRVVLIAGVLGMFGV
                     ESTSFAALLAAAGVAIGMAWAGLLANFAAGVFLVLLRPFKVGDVITAAGVSGLVTEIG
                     LFATTLDTRDNVRIFVGNNKLFGDNIINHSHNPYVRTELIAQVANGVDPFAIVARLTP
                     LIAQIPHVMSDPAPEVGVREFNAAGTVIYIRPYVLGGGAEVEAVKGAGYRAIAEATAG
                     LPVPAPYQVLLQQPQR"
     gene            2180..>2845
                     /locus_tag="VGB85_32525"
     CDS             2180..>2845
                     /locus_tag="VGB85_32525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138872.1"
                     /translation="MLTPARTLLLSCLMAGTALVACGSTLAGIVRSRAAGDLGCPENQ
                     LAVRDMHTGDAVRDFSVSGCGRQEHYQAACDPKGTCQVVRPGDATRYPAPPGDARTAG
                     VEDPKPADEGVTTVVDEGVTTVVAEAPPEAEPEGQGEAGSIAAGDEAARGEAMAIAKD
                     LPPAGTPSTAPASKLPPAEERTVTLRNNCARRVVFYIGKQPGESDGRYMTVGSTNMIS
                     PRLR"
BASE COUNT          449 a          926 c         1102 g          368 t
ORIGIN      
        1 gcgcttcgcg cgggcggtgc tcgatcaccg cgaggcccgc accgcgatct acccggtcgg
       61 cgcgagcggg gtcggcagcg acgcggaggc cgagcttcgg ctcgccgcca aggcgaccgg
      121 cggacagtac atcttcctca ccgaccactc gggcgtcggc ggtcatcacg aggcagccaa
      181 ggtcgagcag tacaaggtcg agaccctgca cgacgcgatg acccggatga tccgcggcga
      241 gctcggcgat gccaaggtcc aggcccggac cgcaccgctc gagacctgtg acgagacgcc
      301 gatgccgatg ccgacgccgg tcgcgctcga cgcgagcgcg ctggtgccgg tcgaggcgcc
      361 cgcggtcagc ccgtgggtgc agctgcgcga gcgcctcgag ggcaacctgc tgttcgccct
      421 cggcatgctc gtgatggtgc tcgcagggat cggcttcgac acgctcattc accgctggcg
      481 cacccgctga ggtaggcgcg ccggccccgg cgggcccgct gcagccaaag cgcgggccgc
      541 tggcccgcgg gccgcggtca cggggtcggg gactcgaggc gcaggaagtc gatcagatac
      601 agcgagcgcg ccgagatccg ccagccctgc ggggtgcgga cgacggtgtc gacgtaggtg
      661 ccgtgcgcga ggtcgacggc gccgtcgggg tcgacgacgt gggtcgcgct gaggtaggag
      721 cgcatcgtcc cgctgttgcc gtcgaggctg atgcggatgt tgctcatcgc gtgctgggtg
      781 gcgatgtagc cgttgccggt gaacacggag gcgacggcgt cggcccacgc gtcggtgccg
      841 gtggcgctga ggccgggcgg gccgttgaag tcggcgcccg ggaaccacgc cttgacggtg
      901 gcgccggcgg tgaagcagtc gccgtagacc gccttgccgg cggcgaggtc accaccaccg
      961 atcgcgtcgg tgccctcggc gtaacagtag gcgagctcgg cgatctcggc gcggtcggac
     1021 agacgcttgc cgacctcgtc ctgcgcacca ttcttgccat tgacggcgga cgcgatgaag
     1081 aggccggcgg cgcacacgag agaggccgcg aacacgcgat gaattgcagt taaacgacgt
     1141 tccatggccg gagtgtagag gaactcgcgg tgcatgggcg ctcgctggaa cgcggccatc
     1201 gcgtgctcgc gggtcgcatc agcgctgcgg ctgctgcagc agcacctggt agggggcggg
     1261 cacgggcagg ccggcggtcg cctcggcgat cgcacgatag cccgcgcctt tgacggcctc
     1321 gacctcggca ccgccgccga gcacgtaggg acggatgtag atgacggtgc cggcggcgtt
     1381 gaactcgcgc acaccgacct cgggcgcggg gtcggacatc acgtgcggga tctgggcgat
     1441 gagcggggtg aggcgggcga cgatcgcgaa gggatcgacg ccgttggcga cctgcgcgat
     1501 cagctcggtg cgcacgtagg ggttgtgcga gtggttgatg atgttgtcgc cgaacagctt
     1561 gttgttgccg acgaagatcc ggacgttgtc gcgggtgtcg agcgtggtgg cgaacagacc
     1621 gatctcggtc accaggccgc tgacgccagc ggcggtgatg acgtcgccga ccttgaaggg
     1681 gcgcagcagc acgaggaaca cgccggccgc gaagttggcg agcaggccgg cccacgccat
     1741 gccgatcgcg acgccggccg cggcgagcag ggcggcgaag gaggtcgact cgacgccgaa
     1801 catgccgagc acgccggcga tcaggacgac gcgcagcagg atgctcagcg cggaggtgac
     1861 gtagctggtc agcgtgcggt cgaggttgcg agccgtcatc gcggtgccga ccatgcgaca
     1921 cgcgaagcgg atcaagatcc cgccgacgat ccagatgccg atcgcgccga gcacctgcca
     1981 gaggaagggg atcgcgtaca gcttgaacac ctccgacgcg gtgacgaacc agtcggcagc
     2041 gggtgtggag gacgcggtgg tggtggcagg actctcgaga tacatcgtgg gccgcacgat
     2101 ggcgggagcg gcgcccgctt gcaagcccgg tggatcaccc gggacggccc tggcgctggc
     2161 catgataaaa ccgcggcgga tgcttacgcc cgcgcgcacg ctcctgctct cgtgtctgat
     2221 ggccggcacc gcgctcgtcg cctgcgggag cacgctggcc ggcatcgtgc gcagccgggc
     2281 cgcgggcgac ctcggctgtc ccgagaatca gctcgcggtg cgagacatgc ataccggcga
     2341 cgcggtgcgt gacttcagcg tcagcggctg cggccgccag gagcactacc aggccgcctg
     2401 cgacccgaag ggcacctgcc aggtggtccg ccccggcgac gccacgcgct accccgcgcc
     2461 ccccggtgac gcgcggacgg ccggcgtgga ggacccgaaa ccagccgacg agggcgtgac
     2521 gacggtggtc gacgagggcg tgacgacggt ggtcgccgag gcgccgccgg aggccgagcc
     2581 cgaggggcag ggggaggcgg gcagcatcgc ggcgggtgac gaggcggcgc gcggcgaggc
     2641 gatggcgatc gcgaaggatc taccgcccgc gggcacaccg agcacggcgc ccgcgagcaa
     2701 gctgccgccg gccgaggagc ggacggtgac gctgcgcaac aactgtgcgc ggcgggtggt
     2761 gttctacatc ggcaagcagc cgggcgagag tgacgggcgt tacatgacgg tcggctcgac
     2821 caacatgatc tccccgcgcc tgcgc
//
LOCUS       DASWNJ010001954         4728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_417100, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001954 DASWNJ010000000
VERSION     DASWNJ010001954.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417100"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4728)
                     /locus_tag="VGB85_32530"
     CDS             complement(<1..>4728)
                     /locus_tag="VGB85_32530"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY0138873.1"
                     /translation="KVGDRVQLFHGAHAWAEYELKRRDPKLREVSLRMLASWNDADSL
                     DLLGARVSQDEDHGLKVLATELLGASGSPRAVKLLEPLLQAGEEAVRMAALAGLRKLE
                     GPTSLRVLDLALAAQKRDVGVAAVEALTELARKDDLALARLTAALGQNPREVRLAALV
                     ALEALHPDKSPEAELVALRAKQADIRRTALIRFYQRDRLDEAEVQAALRRFSAVADAG
                     VRQAAFLVSLLATPKLAAALRFRDKQIHRLLHDIETAPDPTAEVKAAAAKKESKKDSK
                     PSDDDLPKAKKVDIADVSEADKRPLLEAMASRALDTCLAGATGLAALQDPRALGTLLQ
                     LSRETTASVRVETCKALAELGDPRGAARLRLMLRDSDASVRDAAFTALVRLEDSVPLA
                     VAAAGLGAEHEDVRRRGLDLLVKQIKKSREPAAISLLERALGDAGKTVRSEAFKAALS
                     LEVGGPAAGSLRFALKSLHADVRREVLNEVIGQISQAWAWPLLIELLGDPDPGVRKEA
                     FDFALKRTKGIGLEPLSAALAGKYADLRVEATRVLAKRKAAGTHELLVKAVDDEDETV
                     RQLAVDALLVAEGDEALTAAMASKHPDVVVRAAGARAVHGDSSARAPLVTMIAEPEPE
                     TPALRPKWLDRVVRALAGLAELGDPAASSAVLPLLQHKEAKIRQAAATALAWMARPGN
                     SNAIFALQAALQHSDPAVQAEAALGLASCGDATGASIIFTAGKNAPSPEQGLLAALAL
                     GADDAFIAYLDHAKDRLRDKALLLVLMREMVEGDGVPDRCLAALASAHPRVRLVAAQA
                     LEHFGDDAAFAKFVTDMVNDRGEGKTRWTIAESTVRTIAEVVTFGDGKGNPQLKVRAA
                     RLLDALAEDKQETFDRQWATFSRRFATKIAEVHQQASKRPPSPPAYAPEELRQVVFGA
                     YAALSRLPGGNAEMRIRQTAISRLLASASAGKSKKAPDISAVQSVLVLALGDPQQAVR
                     KQAFDGLKAIGMEPAALAGEALSTGQRDTGALGLALLAQLPGAGKGAKGLKVLEDVLV
                     NRDDGLEQEAAKLLATLLIAQATAGTKTAAPAEGESEAVTAARRDAWVRVHTLALGAR
                     SEALRQAAVSGLAGHYEADGQGPAAQALRGALGSKYRAQRFAAAEQLATKKDAAAFDV
                     LVEKLRSDQANEQSEAIRALQRLGDPRATAAFMNRIDDDPAGSARVDELIKAVGGFAQ
                     VSDVPRVLAYVDTPKRRAAAFTALLAISGHDQRIEDPEDEGALGSSTPVIVADDDDDD
                     NDYDDDDDDDDDDDISVDDDDDDDAPAAFQADWEKKQRPRHDEVLVQIAEAARRLSDD
                     RQLQAIALPMRWARSKELDAALAPLLTYPKPEIARKATEAIGWRLRKRGGPAEPLLGA
                     LKSPDPELAFLAAEGLALAGRGDGVGVLLTSVDLMPDLNLRKRAVKALGALADIRALD
                     TLLRFVGEDGHALQDEAAEALGHLGQSGKAGEQPGQADKIFGILSKLAAGSGTAAQRA
                     LVGLRWFGTRDAWGLVRGRARDENFGVRQTVARLLEFDADPHTRELIGERLRVETYAG
                     IASALAHSLRKLYGADSLEPDYVF"
BASE COUNT          569 a         1741 c         1720 g          698 t
ORIGIN      
        1 gaacacgtag tcgggctcga gcgagtcggc gccgtacagc ttgcgcaggc tgtgcgccag
       61 cgcgctggcg atcccggcgt aggtctcgac gcgcaggcgc tcgccgatca gctcgcgcgt
      121 gtgcgggtcg gcgtcgaact cgagcaggcg ggcgaccgtc tggcgcacgc cgaagttctc
      181 gtcgcgggcc cgtccgcgca cgaggcccca cgcgtcgcgc gtgccgaacc agcgcaggcc
      241 gacgagggcc cgctgcgcgg cggtacctga ccccgcggcc agcttcgaga ggatcccgaa
      301 gatcttgtcg gcctggcccg gctgctcgcc ggccttgccg ctttgaccaa ggtgcccgag
      361 cgcctcggcg gcctcgtcct gcagcgcgtg cccgtcctcg ccgacgaagc gcagcagggt
      421 gtcgagcgcg cggatgtcgg ccagcgcgcc gagggccttg accgcccgct tgcgcaggtt
      481 gaggtccggc atcaggtcga cgctggtaag tagcacgccg accccgtcgc cgcgacccgc
      541 gagcgcgagg ccctcggccg cgaggaacgc gagctccgga tcgggtgact tgagggcgcc
      601 gagcagcggc tccgccggcc cgccgcgctt gcgcaggcgc cagccgatcg cctcggtcgc
      661 cttgcgggcg atctccggct tcgggtaggt cagcagcggc gcgagggcgg cgtcgagctc
      721 cttgctgcga gcccagcgca tcggcagcgc gatcgcctgc agctggcggt cgtcggacag
      781 acggcgcgcc gcctcggcga tctggaccag cacctcgtcg tggcgcgggc gctgcttctt
      841 ctcccagtcg gcctggaagg ccgcgggcgc gtcgtcgtca tcatcgtcgt cgacgctgat
      901 gtcgtcgtcg tcatcgtcgt cgtcgtcgtc gtcgtaatca ttatcatcat cgtcatcatc
      961 ggcgacgatg accggcgtgc tgctgccgag ggccccctcg tcctccgggt cctcgatgcg
     1021 ctggtcgtgg ccggagatcg ccagcaacgc ggtgaaggcc gcggcgcggc gcttgggcgt
     1081 gtcgacgtag gcgagcacgc gcggcacgtc actgacctgc gcgaagcctc cgaccgcctt
     1141 gatcagctcg tcgacgcgag cgctgccggc cgggtcgtcg tcgatgcggt tcatgaaggc
     1201 cgcggtcgcg cgcgggtcac cgaggcgctg cagggcgcgg atcgcctcgc tctgctcgtt
     1261 ggcctggtcc gagcgcagct tctcgacgag cacgtcgaag gcggccgcgt ccttcttggt
     1321 cgccagttgc tccgcggccg cgaacctctg cgcgcggtac ttcgagccga gcgcgccgcg
     1381 cagggcctgc gccgcgggcc cctgcccgtc ggcctcgtag tgcccggcga gccccgagac
     1441 agccgcctgg cgcagcgcct ccgagcgggc cccgagcgcg agcgtgtgca cgcggaccca
     1501 tgcatcacga cgcgcggcgg tcaccgcctc gctctcgccc tcggccggcg cggccgtctt
     1561 cgtcccggcg gtcgcctggg cgatcagcag cgtcgccagc agctttgcgg cctcctgctc
     1621 gaggccgtcg tcgcggttga cgagcacgtc ctcgagcacc ttgaggccct tggcgccctt
     1681 gcccgcgccg ggcagctggg cgagcaacgc gaggccgagc gcgccggtgt cgcgctggcc
     1741 ggtgctcagc gcctcgccgg cgagcgcagc cggctccatc ccgatcgcct tgagcccgtc
     1801 gaaggcctgc ttgcgcaccg cctgctgggg gtcgcccagc gcgagcacca gcacgctctg
     1861 gaccgccgag atgtcgggcg ccttcttcga cttgccggcg gacgcggacg cgagcagccg
     1921 cgagatcgcg gtctggcgga tgcgcatctc ggcgttgcca cccggcaggc gcgacagcgc
     1981 cgcgtaggcg ccgaacacga cctgacgcag ctcctcgggc gcgtaggccg gcgggctcgg
     2041 cgggcgcttg ctcgcctgtt gatggacctc cgcgatcttg gtggcgaagc ggcgggagaa
     2101 ggtcgcccac tggcggtcga aggtctcctg cttgtcctcg gcgagcgcgt ccagcagccg
     2161 ggccgcgcgg accttgagct gcgggttgcc cttgccgtcg ccgaacgtga cgacctcggc
     2221 gatcgtccgc acggtcgact cggcgatcgt ccacctggtc ttgccctcgc cgcggtcgtt
     2281 gaccatgtcg gtcacgaact ttgcaaaggc cgcgtcgtcg ccgaagtgct cgagcgcctg
     2341 cgcggccacc agccgcaccc gcggatgcgc cgaggccagc gcggcgaggc agcgatccgg
     2401 cacgccgtcg ccctcgacca tctcgcgcat cagcaccagc agcagcgcct tgtcgcgcag
     2461 gcggtccttc gcgtgatcga ggtaggcgat aaacgcatcg tcggccccga gcgcgagcgc
     2521 tgcgagcagg ccctgctcgg gcgacggggc gttcttgccg gcggtgaaga tgatcgacgc
     2581 gccggtcgcg tcaccgcagg acgcgagccc gagcgccgcc tcggcctgca ccgcggggtc
     2641 gctgtgttgc agcgcggcct gcaacgcgaa gatcgcgttg gagttgccgg gccgggccat
     2701 ccacgccaaa gcggtcgccg cagcctgccg gatcttcgcc tccttgtgtt gcagcagcgg
     2761 cagcacggcc gagctcgccg cggggtcgcc gagctcggcg aggccggcga gcgcacgcac
     2821 gacgcggtcg agccacttcg ggcgcagcgc gggcgtctcg ggctcgggct cggcgatcat
     2881 cgtgaccagc ggcgcgcggg ccgagctgtc gccgtggacc gcccgcgctc ccgcggcgcg
     2941 cacgaccacg tccgggtgct tgctcgccat cgcggcggtc agcgcctcgt cgccctcggc
     3001 gaccagcagc gcgtcgacgg ccagctggcg cacggtctcg tcctcgtcgt cgaccgcctt
     3061 gaccagcagc tcgtgcgtgc cggcggcctt gcgcttggcg agcacccggg tcgcctcgac
     3121 gcgcaggtcg gcgtacttgc ccgccagcgc ggcgctcagc ggctcgaggc cgatgccttt
     3181 ggtccgcttg agcgcgaagt cgaaggcctc cttgcgcacc ccgggatcgg ggtcgccgag
     3241 cagctcgatc aagagcggcc aggcccacgc ctgggagatc tggccgatca cctcgttgag
     3301 cacctcgcgg cgcacgtcgg cgtgcaggct cttgagcgcg aagcgcaggc tgccggccgc
     3361 gggcccaccg acctcgaggc tcagcgcggc cttgaacgcc tcgctgcgca cggtcttgcc
     3421 ggcgtcaccg agggcgcgct cgagcagcga gatcgcagcc ggctcgcgcg acttcttgat
     3481 ctgcttgacg agcaggtcga ggccgcggcg gcgcacgtcc tcgtgctcgg cgccgaggcc
     3541 cgcggccgcg accgccaatg gcaccgagtc ctcgaggcgg accagcgcgg tgaaggccgc
     3601 gtcgcgcacg ctggcgtcgc tgtcgcgcag catcaggcgc aggcgggccg cgccgcgcgg
     3661 gtcaccgagc tcggcgagcg ccttgcacgt ctcgacgcgc acgctcgccg tcgtctcgcg
     3721 gctgagctgc aacaacgtgc ccagcgcgcg cgggtcctgc agcgcggcca ggccggtcgc
     3781 gccggcgagg caggtgtcga gcgcgcggct cgccatcgcc tcgagcagcg ggcgcttgtc
     3841 ggcctccgag acatcggcga tgtcgacctt cttggccttc gggagatcgt cgtcgctcgg
     3901 cttgctgtcc ttcttcgact ccttcttcgc ggccgcggcc ttgacctcgg cggtcgggtc
     3961 cggcgcggtc tcgatgtcgt gcagcaggcg gtggatctgc ttgtcgcgga accgcagcgc
     4021 cgcggcgagc ttcggggtcg ccagcaacga cacgaggaac gcggcctggc gcacgcccgc
     4081 gtcggcgacg gcgctgaagc ggcgcagcgc ggcctggacc tcggcctcgt cgagcctgtc
     4141 gcgctgatag aagcggatca gcgcggtgcg gcggatgtcg gcctgcttgg cgcgcagggc
     4201 caccagctcg gcctccggcg acttgtccgg atgtaacgct tccagggcga cgagcgcggc
     4261 gagacggacc tcgcgcgggt tctgcccgag cgcggccgtc aacctcgcca gcgcgaggtc
     4321 gtccttgcgc gcgagctcgg tcagggcctc gaccgccgcg acgccgacgt ctcttttctg
     4381 ggccgcgagc gccaggtcga gcacgcgcaa tgaagtgggc ccctcgagct tgcgcagccc
     4441 ggcgagcgcc gccatgcgca cggcctcttc accggcttgc agcagcggct cgagcagctt
     4501 gaccgcgcgc ggagatcccg aggccccgag cagctcggtc gccagcactt tgagcccgtg
     4561 gtcctcgtcc tggctgacgc gcgcgccgag caggtcgagc gagtcggcgt cgttccagga
     4621 cgccagcatg cgcagcgaga cctcgcgcag cttcgggtca cgccgcttga gctcgtactc
     4681 ggcccacgcg tgcgcgccgt ggaacagctg cacgcggtcg ccgacctt
//
LOCUS       DASWNJ010001955         2021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_528635, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001955 DASWNJ010000000
VERSION     DASWNJ010001955.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528635"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VGB85_32535"
     CDS             complement(<1..224)
                     /locus_tag="VGB85_32535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138874.1"
                     /translation="MSTGFQTLQVTSSCTGLHVVLGGRPGALAASGDGWRGQGLEFAL
                     ATLIGEHEAGGEAVTTVTLKLARTEDAFWA"
     gene            438..1790
                     /gene="pcnB"
                     /locus_tag="VGB85_32540"
     CDS             438..1790
                     /gene="pcnB"
                     /locus_tag="VGB85_32540"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEY0138875.1"
                     /translation="MADGKAGTRGAISLRDVDPDALKVVRKLVAGGFEAYLVGGCVRD
                     LYLKRRPKDFDVATSATPEALRRTFRNCRIIGRRFKLAHVFFGPKVIETSTFRTTPIA
                     AEDDPLITHDNEWGTVEDDARRRDFTINGLFYDLDADAIIDFVDGLSDLDRGVIRTIG
                     DPLLRFQEDPVRMIRAIKFAARLDFTIETDTWKALLATVQDIAKCSRARVLEEIYKLM
                     RGGGARRSFELLLETQLLQHLMPSYLELFEKLAPPGLQVPSRSPPGTPGRLLWDLLGA
                     LDEFTGATHQIADNGVLLSVLFAPLIDDQWMTASRQGLDQMIDDLMMPFCFALGIARR
                     DRELARQILMAHRRMVEAVTRKKRRPSLVQRQYFHDALVFLGLSVKARGGDGNELLHW
                     QRLAAASHGGSAPFATGDLERSDDDATGPRKRRKKRSRLRRDAPERPRVAAGATRERG
                     "
     gene            1790..>2021
                     /locus_tag="VGB85_32545"
     CDS             1790..>2021
                     /locus_tag="VGB85_32545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138876.1"
                     /translation="MPKQSGDPPSRPHPDPDTLLRRGTSDHYEDAELYDFEYRDHGHD
                     VTWYRNLAKKLAARRADPLAGPLKVLELGAGTG"
BASE COUNT          316 a          782 c          639 g          284 t
ORIGIN      
        1 tgcgcccaga acgcgtcctc cgtgcgggcc agcttgaggg tcaccgtggt gaccgcctcg
       61 ccgccggcct cgtgctcgcc gatcagggtc gccagggcaa actccagccc ctgcccccgc
      121 cagccgtcgc ccgacgccgc cagggcgccc gggcgtccgc cgaggaccac gtggaggccg
      181 gtgcacgagg aggtcacttg cagggtctgg aatccggtgc tcatggtcag gttgctcgcc
      241 tgttcagtaa actgcgcccg ggcccgagct gagtcaacgc cgcgctttat ctggatgaga
      301 tcaagtgccc ccggacccct gaacagaccg cgcgacatcg cgcgcctcgt tcccgctgtc
      361 tccccggtaa cgagcgggca cacgcacgag gtttcgcccg tgccggggcg agtgtgttag
      421 agcagcaccg catcagcatg gccgacggca aagcaggcac gagaggcgcc atctcccttc
      481 gcgacgtgga cccggacgca ctgaaggtgg tccgaaagct ggtcgccggg ggcttcgagg
      541 cctacctggt cggaggttgc gtgcgcgacc tctacctcaa gcgccggccc aaggacttcg
      601 acgtcgccac cagcgcgacg ccggaggcgc tgcgccgcac ctttcgcaac tgccggatca
      661 tcgggcgccg cttcaagctc gcgcacgtgt tcttcggccc gaaggtgatc gagaccagca
      721 cctttcgcac caccccgatc gcggccgagg atgacccgct gatcacccac gacaacgagt
      781 ggggcacggt cgaggacgac gcgcgccggc gcgacttcac gatcaacggc ctgttctacg
      841 acctcgacgc cgacgcgatc atcgacttcg tcgacggcct gagcgacctc gaccgcggcg
      901 tgatccggac gatcggcgac cccctcctgc gcttccagga ggacccggtc cgcatgatcc
      961 gcgcgatcaa gttcgccgcg cgcctcgact tcacgatcga gaccgacacc tggaaggccc
     1021 tgctcgccac cgtgcaggac atcgccaagt gctcgcgcgc ccgcgtgctc gaggagatct
     1081 acaagctcat gcgcggcggc ggggcccggc gcagcttcga gctgttgctc gagacccagc
     1141 tcctgcagca cctgatgccg agctacctcg agctgttcga gaagctcgcg cccccgggcc
     1201 tgcaggtgcc ctcgcgctcg ccccccggca cccccggccg cctgctgtgg gacctgctcg
     1261 gcgcgctcga cgagttcacc ggcgcgacgc atcagatcgc cgacaacggc gtgctgctga
     1321 gcgtgctgtt cgccccgctg atcgacgacc agtggatgac cgcctcgcgc cagggcctcg
     1381 atcagatgat cgacgacctg atgatgccgt tctgcttcgc gctggggatc gcccgccgcg
     1441 accgcgagct cgcccgccag atcctgatgg cccaccgacg catggtcgag gccgtcaccc
     1501 gcaagaagcg gcgcccctcg ctggtccagc gccagtactt ccacgacgcc cttgtcttcc
     1561 tcggcctctc ggtcaaggcc cgcggcggcg acggcaacga gctgctgcac tggcagcgcc
     1621 tcgccgcagc ttcacacgga ggcagcgcgc cgttcgccac cggcgacctc gagcgctccg
     1681 acgacgacgc caccggcccc cgcaagcgtc gcaagaagcg cagccgcctg cgccgcgacg
     1741 cccccgagcg cccgcgcgtc gccgccggtg ccacccgcga gcgcggctga tgccaaagca
     1801 aagcggcgat ccgccctcgc gtcctcaccc cgatcccgac acgctgctgc gtcgcgggac
     1861 cagcgatcac tacgaggacg cggagctcta cgacttcgag taccgcgacc acggccacga
     1921 cgtcacctgg taccgcaacc tcgccaagaa gctcgcggcc cgccgcgccg atccgctcgc
     1981 cggtccgctc aaggtcctcg agctcggcgc cggcaccggg c
//
LOCUS       DASWNJ010001956         2334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_457220, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001956 DASWNJ010000000
VERSION     DASWNJ010001956.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457220"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VGB85_32550"
     CDS             complement(<1..533)
                     /locus_tag="VGB85_32550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138877.1"
                     /translation="MTLSPRFCATLVALTLATPTAAVASAQTPTVAATPEATAAETPA
                     DSREFVGPEVSEPTSSAAALRAFEGGFDAGQALLERGEPLAAARTWVETVALLPEGPE
                     HRDNRAAVFGYIAEAYERALAGAAPRARLEEALAALDRYADTVKATHGADAPLPERVV
                     TVRAELRARLSAMDRPAT"
     gene            complement(530..1681)
                     /locus_tag="VGB85_32555"
     CDS             complement(530..1681)
                     /locus_tag="VGB85_32555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138878.1"
                     /translation="MNDDKQNMHQRDPHGPRRTPQRLVQAAMSALCAGSLAACFNPTA
                     DGETTGTTHDPAESTSGSSSSGTPTTSGADSSTSVDLTTSTTSTTGTSEPLTGSTSDA
                     TGTTGPALDESYCGDGVLDRELNEVCDDGDFLNNNECTNFCTLPTCGDKFLGPDEACD
                     DGNQEPGDGCDIKCQCERTTCGNGMLERGEVCDDGNISDRDACSACCERLYYYAFVTS
                     QMVVGGEIDSIQDADMRCTQLANAAGLQGPYRAWLAGDRGPAAPAADVFARPYALPGT
                     AEIVAHSLNELRSGQLAAAIHVTEKGEDIGAVPADPCLDAALRVWTGTDSSGTGVWNA
                     TCSNWSTLDLPDLVGRFGAAGTSGPTWASCEMEVDCDMMARLYCFEVQT"
     gene            1810..>2334
                     /locus_tag="VGB85_32560"
     CDS             1810..>2334
                     /locus_tag="VGB85_32560"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0138879.1"
                     /translation="MADDPPDDPLRGLVLEGRFRLERLLGEGGMGVVYIAEELRLQRR
                     CAVKLLSPELGADPIYAERFRREAQAIAALQHDNVIDIYHLGEGPDGLLFFAMELLVG
                     EDLETRIDRRGARPYTWVDCCHWAISIARGTAAVHHAGLVHRDLKPSNVFLARQRDGR
                     EVVKLLDFGIARVQG"
BASE COUNT          308 a          872 c          800 g          354 t
ORIGIN      
        1 gtcgccgggc ggtccatcgc cgacagcctc gcgcgcagct ccgcccgcac cgtgaccacg
       61 cgctccggca gcggcgcgtc ggccccgtgg gtcgccttga ccgtgtcggc gtagcggtcg
      121 agcgccgcga gggcctcctc gaggcgggcc cgcggggccg cgccggcgag cgctcgctcg
      181 taggcctcgg cgatgtagcc gaacacggcc gcccggttgt cgcggtgctc gggcccctcc
      241 ggcagcagcg cgacggtctc gacccacgtg cgcgccgcgg cgagcggctc gccgcgctcg
      301 agcagcgcct gcccggcgtc gaagccaccc tcgaaggccc gcagcgccgc ggcgctgctc
      361 gtcggctcgg agacctcggg gccgacgaac tcgcggctgt ccgcgggcgt ctccgcggcc
      421 gtcgcttcgg gcgtcgccgc gacggtcggt gtctgggcgg atgcgacggc ggcggtgggc
      481 gtcgcgagcg tgagggcgac gagggtcgcg cagaaccggg gcgagagcgt catgtttgga
      541 cctcgaagca gtagagccgg gccatcatgt cgcagtccac ctccatctcg caactggccc
      601 aggtcggtcc ggacgtgccg gcggccccga agcggccgac caggtcgggc aggtccagcg
      661 tcgaccagtt ggagcaagtc gcgttccaga cccccgtccc gctcgaatcg gtgccggtcc
      721 acacgcgcag cgccgcatcc aggcaggggt ctgccggcac cgcccctata tcctcgccct
      781 tctcggtgac gtggatcgca gccgccagct ggccactacg cagctcgttg agtgaatgtg
      841 cgacgatctc cgccgtgccc ggcagcgcgt agggccgcgc gaacacgtcg gctgccggcg
      901 cggccgggcc gcggtccccc gccagccagg cccgatacgg accctgcagg ccggccgcgt
      961 tcgccagctg cgtgcaccgc atgtcagcgt cctgaatgct atcgatctcg ccgccgacga
     1021 ccatctgcga cgtcacgaac gcgtaataat acaggcgctc gcagcaggcc gagcacgcgt
     1081 cgcggtcgct gatattgccg tcgtcacaga cctcgccgcg ctcgagcatg ccgttcccgc
     1141 aggtcgtgcg ctcgcactgg cacttgatgt cgcagccgtc gccgggctcc tggttaccgt
     1201 cgtcgcaggc ctcgtccggg ccgaggaact tgtccccgca ggtcggcagc gtgcaaaagt
     1261 tcgtgcactc gttgttgttg aggaagtcgc cgtcgtcaca gacctcgttg agctcgcggt
     1321 cgagcacacc gtcgccgcaa tacgactcgt cgagcgccgg cccggtcgtc ccggtcgcgt
     1381 ccgaggtcga gcccgtcagt ggttcgctcg tgccggtcgt cgacgtcgtc gacgtcgtca
     1441 ggtcgacgct cgtcgagctg tcggcgccgg aggtcgtcgg tgtaccactc gagctcgacc
     1501 ccgacgtgct ctcggcggga tcatgggtcg tgccggtggt ctcgccgtcg gcggtggggt
     1561 tgaagcaggc ggcgagagac ccggcgcaca gcgccgacat cgcggcctgg accaggcgct
     1621 gcggggtccg ccggggcccg tgggggtccc gctggtgcat attttgcttg tcatcgttca
     1681 ttgcacacct gactgcgatc gggggggaga tcagcatctg tgacgatagt tgcgggcggc
     1741 cccccgaccg ttcacacaaa tcgaccgcgc actcccggac ccacgcgcct cgggtatcat
     1801 cccgccgcga tggcagacga cccccccgac gaccccctgc gcggcctcgt gctcgaggga
     1861 cgctttcgcc tcgagcgcct gctcggcgag ggcggcatgg gcgtcgtcta catcgccgag
     1921 gagctgcgcc tgcaacggcg ctgcgccgtc aagctgctga gccccgagct cggcgccgac
     1981 ccgatctacg ccgaacgctt ccgccgcgag gcccaggcga tcgccgcgct gcagcacgac
     2041 aacgtcatcg acatctacca cctcggcgag ggccccgacg gcctgctgtt cttcgccatg
     2101 gagctgctcg tcggcgagga cctcgagacc cgcatcgacc gccgcggcgc ccgaccctac
     2161 acctgggtcg actgctgcca ctgggcgatc tccatcgccc gcggcaccgc ggccgttcac
     2221 cacgccggcc tcgtccatcg cgacctcaaa ccatccaacg tgttcctcgc ccgccagcgc
     2281 gacggccggg aggtcgtcaa gctgctcgac ttcggcatcg cccgcgtcca gggc
//
LOCUS       DASWNJ010001957         3746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_246395, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001957 DASWNJ010000000
VERSION     DASWNJ010001957.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246395"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VGB85_32565"
     CDS             <1..278
                     /locus_tag="VGB85_32565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138880.1"
                     /translation="VHRAATDRGLIHRVDPDFQVPVRLTWTVGKLGFSFALLQGFSTR
                     TRTYARRGTEVWHRIPYRIGFAVGIHFDVGVGRTRSRRSNREPKGLP"
     gene            299..2083
                     /locus_tag="VGB85_32570"
     CDS             299..2083
                     /locus_tag="VGB85_32570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138881.1"
                     /translation="MSSLVLVGCEDAIDLDPLDDRPPVEAAVRPAPILGGTLTVTDDG
                     LAIAADPDRDLIHVVDIDAGEVRHTIALAPGDQPHRIALGSDGQAHVVLRGAGGIATI
                     DPDAGTITARHKLCPEPRGIAFNDADTSLYVACAGGSLFHLTEAGALLERRELEPDLR
                     DVLIVDGKVKVSLFRSATILDLDGSRMKIPDVDEDSVARVAWRTWVTATGEIAMLHQT
                     ASRKPVPIDPKVDDVVDGGAPYGGGGGGCELGIVNTAITTFGPDGTKTTQLVDSNLTV
                     DAALSPDGNTVAMAVPGVVRPEDKVEVVDKGRNGLGRGEFDSFGPTAATTRPSVRVTA
                     KHDPGCFDETGSLPEIDYGQVTAVAYAASGHLVLQSREPARLLVRAPNGTAFETIALN
                     GEPRFDTGHEIFHRATDSGLSCASCHPEGTDDGHVWRFESLGLRRTQALDVGLADTAP
                     YHWDGDMQDFSVLMSEVLAHRMGGKRQSPARRDSFTRWIFEQHRPAAAGGGDLTLAST
                     GEALFTSYGCNTCHDGPALGGRRTERIAGADKQVPTLRRISLHPPFMHDGRASTLEDA
                     VRDMITTTRPTSAPNEDIEAIAAYLRTL"
     gene            2129..2911
                     /locus_tag="VGB85_32575"
     CDS             2129..2911
                     /locus_tag="VGB85_32575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138882.1"
                     /translation="MIVVTGTKRSGTSMWMHVLVAAGLPYIGERFPVGWGDLLRDANP
                     DGFFESELMIGINYRTNPHPMTGAYLAPHQTREHAVKIFIPGLVRSDVAFIDRCIATV
                     RGWREYVLSIRRVGIEDATLPPALEWWCSNFALIRDLAIRGYPAHVLSYDSFLADPER
                     VAAEVLAWIGRGDPAAAARVVRSEKGRERIAEPDSVLADGIDTRHLEVFDELHATIDA
                     GRPLTASFIEQLNAVDRALRPMVREYQATVEAERVADIFGRR"
     gene            2919..>3746
                     /locus_tag="VGB85_32580"
     CDS             2919..>3746
                     /locus_tag="VGB85_32580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138883.1"
                     /translation="MRMVFEPSTLHLRFRNPEATSDGSGPPEPPDVDAFLNGRRAGAD
                     PYMEDELGVLPGPRPVDPAAPLVIFLQNPEFEIPAPGYDHSLPDLTSWSVRPTTFGWI
                     AHVSPPARSGGPRRAAQLVAGCIALTLPFPANALTDAPASAPPPVVAPATASTTTSTT
                     TSTSKAPATSTSGKAPATPPAAAPAPATPAPTPAPASAQPAGSTVPVASGSPPAAIPP
                     PAPRIQDTRAVADAAWEGIDGFLVVVELKGGRTLRGRIGAVQADTFTLIESTDGQILV
                     "
BASE COUNT          538 a         1466 c         1209 g          533 t
ORIGIN      
        1 tcgtccaccg cgcggcgacc gatcgtggcc tcatccaccg cgtcgacccc gacttccagg
       61 tccccgtgcg gctcacctgg accgtcggca agctcggatt cagcttcgcc ctgctccagg
      121 gctttagcac ccgcacccgt acctatgctc gccgggggac cgaggtttgg catcgcattc
      181 cctaccgcat cggcttcgcg gttgggatac acttcgatgt cggggtcgga cggacgaggt
      241 cgcgccgctc caaccgcgaa ccgaaagggc tgccatgact tacttgcgtc tacttgcctt
      301 gtcctccctg gttctcgtcg gctgcgaaga tgcgatcgat ctcgatccgc tcgatgacag
      361 gccgcccgtc gaggctgcgg tccggccggc gccgatcctc ggcggcacgc tgaccgtcac
      421 cgacgacggc ctggcgatcg ccgccgaccc cgaccgtgac ctgatccacg tcgtcgacat
      481 cgacgccggc gaggtccgtc acacgatcgc tctggccccc ggcgatcagc cgcaccggat
      541 cgctctcggg agcgacggcc aggcccacgt cgtcctgcgc ggcgccggtg ggatcgccac
      601 catcgacccg gacgccggca cgatcaccgc ccgccacaag ctgtgccccg agccccgcgg
      661 gatcgccttc aacgacgccg acacctcgct gtacgtcgcc tgcgccggcg gttcgctgtt
      721 ccacctcacc gaggccggcg cgttgctcga gcgccgggag ctcgagcccg acctgcgcga
      781 cgtcctgatc gtcgacggca aggtcaaggt ctcgctgttt cgttcggcca cgatcctcga
      841 cctcgacggc tctcgcatga agatccccga tgtcgacgag gactcggtcg cgcgcgtcgc
      901 gtggcggacc tgggtgactg ccaccggcga gatcgcgatg ctgcatcaga ccgcgagccg
      961 gaagcccgtg ccgatcgacc cgaaggtcga cgatgtcgtc gatggcggcg cgccgtacgg
     1021 cggcggcggc ggcggttgcg agctcggcat cgtcaacacc gcgatcacga cctttggccc
     1081 ggacggcacg aagacgaccc agctggtcga ctcgaacctg accgtcgacg ccgcgctctc
     1141 gcccgacggc aacacggtgg cgatggcggt ccccggcgtc gttcggcccg aggacaaggt
     1201 cgaggtggtc gacaagggcc gcaacggcct cggccgcggc gagttcgatt cgttcgggcc
     1261 gaccgccgcg accacgcgcc cctcggtgcg ggtcaccgcc aagcacgacc ccggatgctt
     1321 cgacgagacg ggctcgctgc ctgagatcga ctatggccag gtcaccgcgg tggcgtacgc
     1381 cgcgagcggc cacctggtgc tgcagagccg cgagcccgcg cgcttgctgg tgcgggcgcc
     1441 caacggcacg gccttcgaga cgatcgcgct caacggcgag ccgcgcttcg acaccggcca
     1501 cgagatcttc catcgtgcca ccgactcggg gctgagctgc gcgtcgtgcc accccgaggg
     1561 caccgacgac ggccacgtct ggaggttcga gagcctcggc ctgcgccgca cccaggcgct
     1621 cgacgtcggc ctcgccgaca ccgccccgta ccactgggac ggcgacatgc aggacttcag
     1681 cgtgctgatg tccgaggtcc tcgcgcaccg catgggcggc aagcgccagt ccccggcgcg
     1741 tcgcgactcg ttcacccgct ggatcttcga gcagcaccgg ccggcggctg ccggtggcgg
     1801 cgacctgacg ctcgcctcca cgggcgaggc cctgttcacc tcctacgggt gcaacacctg
     1861 ccatgacggg cccgcgctcg gcggccggcg cacggagagg atcgccggcg cggacaagca
     1921 ggtccccacc ctgcgccgca tctcgctgca cccgccgttc atgcacgacg gccgcgcctc
     1981 gaccctcgag gacgccgtcc gcgacatgat cacgacgacc cgcccgacct ccgccccgaa
     2041 cgaggatatc gaggcgatcg cggcctatct gcggacgctc tgagctttcg cccggtcacc
     2101 acccggctcg ccacggaagg caaaccgcat gatcgtcgtc acgggaacca agcgctccgg
     2161 cacctcgatg tggatgcacg tgctggtcgc ggccggcctc ccgtacatcg gcgagcgctt
     2221 cccggtcggc tggggtgatc tcctgcgcga cgccaacccg gacggcttct tcgagtccga
     2281 gctgatgatc gggatcaact atcggaccaa cccgcacccg atgacgggcg cctatctggc
     2341 gccgcaccag acccgcgagc acgcggtcaa gatcttcatc cccgggctcg tgcgcagcga
     2401 cgtcgccttc atcgatcgtt gcatcgcaac cgtccgcggc tggcgggaat acgtcctgtc
     2461 gatccgccgg gtcgggatcg aggacgccac cttgccgccg gcgctcgagt ggtggtgctc
     2521 caatttcgcg ctgatccgcg acctggcgat ccgcggctac ccggcccacg tcctgtccta
     2581 cgacagcttc ctcgccgacc cggagcgggt cgcggccgag gtgctcgcct ggatcggccg
     2641 cggcgacccg gcggccgcgg cccgggtcgt ccgctccgag aagggacgcg agcggatcgc
     2701 cgagcccgac agcgtgctcg ccgacggcat cgacacccgc cacctcgagg tcttcgacga
     2761 gctccacgcc accatcgacg ccggccggcc gctgaccgcg agcttcatcg agcagctcaa
     2821 cgccgtcgac cgggcgctgc gaccgatggt ccgcgagtac caggcgaccg tcgaagccga
     2881 gagggttgcc gacattttcg ggcggcggtg ataccatcat gaggatggtg ttcgagccct
     2941 ccactcttca cctgcggttc cgcaaccccg aagcgacttc tgacggatcc ggcccgccgg
     3001 agccgcccga cgtcgacgcg ttcctcaacg gtcgtcgggc cggcgccgac ccgtacatgg
     3061 aggacgagct cggcgtgctc ccggggcccc gcccggtcga tccggccgct ccgctggtga
     3121 tcttcctgca gaaccccgag ttcgagatcc cggccccggg atacgaccac agcctccccg
     3181 acctcaccag ctggagcgtc cgcccgacga ccttcggctg gatcgcccac gtctcgccgc
     3241 cggcccgcag cgggggcccc cggcgcgccg cccagctggt cgccggctgc atcgccctca
     3301 ccctgccctt ccccgccaac gccctcaccg acgcgcccgc gagcgcgcca ccgcccgttg
     3361 tggcgcccgc caccgcgtcg accaccacgt cgaccaccac gtcgaccagc aaggcgcccg
     3421 ccacctcgac gtccggcaag gcgcccgcga ccccgccagc ggccgcgccg gcgcccgcca
     3481 ccccggcccc gacgcctgcc cctgcctcgg cccagccggc ggggagcaca gtaccggtcg
     3541 catccgggtc gccgccggct gcgatcccgc cgccggcgcc tcgcatccag gataccaggg
     3601 ccgtggccga tgccgcctgg gaggggatcg acggcttcct cgtcgtcgtc gagctcaagg
     3661 gcggcaggac cctgcgcggt cgcatcggcg ccgtccaggc cgacacgttc accctgatcg
     3721 agagcaccga cgggcagatc ctcgtg
//
LOCUS       DASWNJ010001958         4871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_139708, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001958 DASWNJ010000000
VERSION     DASWNJ010001958.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139708"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VGB85_32585"
     CDS             <1..847
                     /locus_tag="VGB85_32585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138884.1"
                     /translation="GNGELDPGEQCDKGHGNNSDSTSYCTLHCQVAVCGDGLVHVGVE
                     ACDHGIDNNDFLYDACGTDCARTKFCGDGEVNGPEECDRGEANGSDDKDADSVPCSAS
                     CTHASLLVFLSSVTYTVYELGGSAYEADGRCRDLAEAGMLANHQNFKAWISDKFSSPF
                     DRFTPTPGLPNALKNGLRVADDHAQQITTGPLTGITITEKGETIYNAKVWTDTKPDGT
                     VFDPTLACENWTTNSALKKARVGRSGVDKADLAEWNKFKSEQQWTTYLTLGCHSKYHL
                     YCFEQ"
     gene            860..1729
                     /locus_tag="VGB85_32590"
     CDS             860..1729
                     /locus_tag="VGB85_32590"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0138885.1"
                     /translation="MHRILFVLSFLLLAACGVEELEAILAKYDTYGSTGSTGGADTSS
                     DTSSSTGPDTTDSPGSTTTEDPGTAGADAGMDTSSTTTPSDTGESTTTGPAEPFCGDG
                     FVDPGESCDDMNDNPDDGCKLCTKDRFVFASSEEYWGYDLDGLYGADQRCRSLAAIAL
                     LPNFATYRAWLSDSTTAAADRITHSKGRYTLVNGLVVAADWDALISGTLENPINVTES
                     SGVSVGSAVWTGTLPNGQPAFGNSFCNGWSEWNGGDEEWAGEGLRNQTDAWWSYFEQA
                     PCGSDMAIYCFEN"
     gene            1897..2727
                     /locus_tag="VGB85_32595"
     CDS             1897..2727
                     /locus_tag="VGB85_32595"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEY0138886.1"
                     /translation="MRTRHFADTNTGRRRKRNEDAYLGDDGLGLFLVCDGVGGRARGE
                     VASAEAAELIWEWVKREEALLRSAIAESMANARSIAVGAEPSPRSAPRLRMSDATIGR
                     LGAMVRSAVQNACYMVHSMGEVDPEHRGMSTTASVAQFVGELIVVAQVGDSRVYLARH
                     GEVTQLTEDHTWLNFQVQHGLVAADQARPGAGKNIITRAVGLRDHVEVDILVVPVQPG
                     DRLLLCSDGLHTYLGAGTILADLFKLDVRAAARAAIHHANACGGADNITALFVEFLAT
                     "
     gene            complement(2729..3910)
                     /locus_tag="VGB85_32600"
     CDS             complement(2729..3910)
                     /locus_tag="VGB85_32600"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0138887.1"
                     /translation="MTHAIIIGGGIAGPVTALALQRAGVTSTVYEAHAGRDDAGGWLT
                     IAVNGLAALRSLDLHRPVMAAGFPTTTIGFLSGTGKPLGAVPIGGVLADGTVTHTLRR
                     SELHRVLREELQRRGPALERGKRLVSAQEADGGVVARFADGSEARGDLLIGADGIHSR
                     VRQILDPGAPRPRYTGLGNMGGFTPAGRVDVPSGACTMVFGRRCFFGMVVAPDGEVWW
                     FANPPRTTEIDRGELQSTSDEQWRERLAAMFDGDRTPAAAVIRASLGPVIAGNQYDLP
                     SVPVWRRGKLLIVGDAAHAAGPSSGQGASMAIEDAVVLARCMREHAEIEDAFAAYEQG
                     RRARVERVVAHGARVGSTKTVGPLGRVLRDLMLPLFLRRAAREQGSGSMAWLFEHHAA
                     V"
     gene            4020..4535
                     /locus_tag="VGB85_32605"
     CDS             4020..4535
                     /locus_tag="VGB85_32605"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0138888.1"
                     /translation="MARKRTRDRSELIEAVDAGGRALSTAAVLFHAALAARQGLTSTE
                     TKALDLLARLGPLTAGALGEQAGLAPASVTGLLDRLERKGFARRIRDPEDGRRVLVEV
                     VPDALAAFAPDFEDLMRELHALYEDFTAEQLETIAHFLNEAARRQSDATMRLAAGPVA
                     PRKQAARRRGD"
BASE COUNT          791 a         1889 c         1496 g          695 t
ORIGIN      
        1 cggcaacggc gagctcgatc ctggcgaaca gtgcgacaag ggccacggca acaacagcga
       61 ctcgacctcc tactgcacgc ttcactgcca ggtcgccgtc tgcggcgacg gcctcgtgca
      121 cgtcggcgtg gaggcgtgcg accacggcat cgacaacaac gattttttgt acgacgcgtg
      181 cggtaccgac tgtgcgcgta caaaattttg cggtgacggc gaggtcaacg gtcccgagga
      241 gtgcgaccgc ggagaggcca acggcagcga cgacaaggac gccgactccg tgccctgctc
      301 ggcgagctgc acccacgctt cgttgctggt gttcctcagc agcgtcacct acaccgtcta
      361 cgagctcggc ggcagcgcct atgaagcgga tggccgctgc cgcgacctcg ccgaggccgg
      421 catgctcgcc aaccaccaga acttcaaggc ctggatcagc gacaagttca gcagcccctt
      481 cgaccgcttc acccccaccc ccggcctccc caacgccctc aagaacggcc tgcgcgtcgc
      541 cgacgaccac gcccagcaga tcaccacggg ccccctcacc ggcatcacga tcaccgagaa
      601 gggcgagacg atctacaacg ccaaggtctg gaccgacacc aagccggacg ggaccgtctt
      661 cgacccgaca ctcgcctgcg agaattggac cacgaactcc gctctgaaga aggcacgcgt
      721 cggccgctcc ggcgtcgaca aggccgacct cgcggagtgg aacaagttca aatcagagca
      781 gcagtggacg acctacctca ccctcggctg ccattccaag tatcacctct actgcttcga
      841 gcagtgaccg ggagccctca tgcaccgcat cctcttcgtc ctctccttcc tgctgctcgc
      901 cgcctgcggc gtcgaggagc tcgaggcgat cctcgccaag tacgacacct acggctcgac
      961 cggctccact ggcggcgccg acacctcgtc cgacaccagc tcgagcaccg gccccgacac
     1021 caccgacagc cccggatcca ccaccaccga agatccaggc acggccggcg ctgacgccgg
     1081 catggacacc agcagcacca ccaccccgag cgacaccggc gagagcacca ccaccgggcc
     1141 cgcggagccc ttttgcggcg acggcttcgt cgaccccggc gagtcctgcg acgacatgaa
     1201 cgacaacccc gacgatggct gcaagctgtg caccaaggac cgcttcgtct tcgcctccag
     1261 tgaagaatat tggggctacg acctcgacgg cctgtacggc gctgaccagc gctgccgcag
     1321 cctcgctgcc atcgccctgc tccccaactt cgccacctat cgcgcctggc tcagcgactc
     1381 cactaccgcc gccgccgacc gcatcaccca ctccaagggc cgctacaccc tggtcaacgg
     1441 cctcgtcgtc gccgccgact gggacgctct catctccggc accctcgaaa accccatcaa
     1501 cgtcaccgag tcctccggcg tctccgtcgg cagcgccgtc tggaccggca ccctccccaa
     1561 cggccagccc gccttcggca acagcttctg caacggctgg agcgagtgga acgggggtga
     1621 cgaggagtgg gccggcgagg gcctccgcaa ccaaaccgac gcgtggtgga gctacttcga
     1681 gcaggcccct tgcggctcgg acatggcgat ctactgcttc gagaactgac cccctctccc
     1741 ccgttcttcc cccctcctcg cccccgggcc cgcatacccc ggcgccgcgc cgcccaagcc
     1801 cgccgcccgt ccactgcccc gaacacagcg ttcccgggcc gacgccaccg cccgcgggtg
     1861 actgtcggtc tgcgcggttg tacgctccgc cccgtgatgc gcacgcgcca cttcgcggac
     1921 accaataccg gtcgccggcg caagcgcaac gaggacgcct acctcgggga cgacggcctc
     1981 ggcctgtttt tggtgtgtga cggcgtcggc ggtcgcgcgc gcggcgaggt cgccagcgcc
     2041 gaggctgcgg agctgatctg ggagtgggtc aagcgcgagg aggcgctgct gcgctcggcg
     2101 atcgccgagt cgatggcgaa cgcccggtcg atcgccgtcg gtgccgagcc atcgccgcgc
     2161 tccgccccgc ggctgcgcat gtccgacgcc acgatcggcc gcctcggcgc catggtccgc
     2221 agcgccgtgc agaacgcctg ttacatggtc cacagcatgg gcgaggtcga cccggagcat
     2281 cgtggcatga gcaccaccgc ctcggtcgcg cagttcgtcg gtgagctgat cgtcgtcgcc
     2341 caggtcggcg actcgcgggt ttacctggcc cgccacggcg aggtcaccca gctgacggag
     2401 gatcacacct ggctcaactt tcaggtccag cacggcctcg tcgccgcgga ccaggcccgc
     2461 ccgggggccg gcaagaacat catcacccgc gccgtcggtc tacgcgacca cgtcgaggtc
     2521 gacatcctcg tcgtgcccgt gcagcccggc gatcgcctgc tgctgtgctc cgacggcctg
     2581 cacacctacc tcggcgcggg cacgatcctc gccgacctgt tcaagctcga cgtccgcgcg
     2641 gccgcccgcg ccgcgatcca ccacgccaac gcctgcggcg gcgccgacaa catcaccgcg
     2701 ctgttcgtcg agttcctcgc gacttaactt aaaccgcggc gtggtgctcg aacagccacg
     2761 ccatcgaccc cgagccctgc tcccgggccg cgcgccgcag gaacagcggc agcatcaggt
     2821 cgcgcagcac ccgtcccagc ggtccgaccg tcttcgtgct gcccacgcgg gccccgtgcg
     2881 cgacgacccg ctccacccgc gcgcgccggc cctgctcgta cgccgcgaac gcgtcctcga
     2941 tctccgcgtg ctcgcgcata cagcgggcga gcaccaccgc gtcttcgatc gccatcgacg
     3001 caccttgccc cgacgacggc cccgccgcgt gggccgcgtc gccgacgatc agcagcttgc
     3061 cgcgccgcca caccggcacc gatggcaggt cgtactgatt acccgcgatc accggcccga
     3121 gcgacgcgcg gatgaccgcc gccgcggggg tgcggtcgcc gtcgaacatc gcggccaggc
     3181 gttcgcgcca ctgctcgtcg ctcgtcgact ggagctcgcc gcgatcgatc tccgtggtac
     3241 gcggcgggtt ggcgaaccac cacacctcgc cgtccggcgc gaccaccatg ccgaagaagc
     3301 agcgtctccc aaagaccatc gtgcaggcgc cggacggcac gtcgacgcgc cccgccggcg
     3361 tgaacccacc catgttgccg aggccggtgt agcgcggccg cggcgcgccc ggatcgagga
     3421 tctggcgcac gcgcgagtgg atcccgtccg ccccgatcaa caggtcgccg cgcgcctcgc
     3481 tgccgtcggc gaagcgcgcc acgaccccgc cgtccgcctc ctgcgcgctc accagccgct
     3541 tgccgcgctc gagcgccggc ccgcgccgct gcagctcctc gcggagcacc cggtgcagct
     3601 cgctgcgccg tagcgtgtgg gtcaccgtcc cgtccgccag caccccaccg atcggcaccg
     3661 cgccgagcgg cttgcccgtg ccgctgagga agccgatcgt cgtcgtcgga aagccggccg
     3721 ccatcaccgg tcggtgcagg tcgaggctgc gcagcgcggc gaggccgttg acggcgatcg
     3781 tcagccagcc gcccgcgtcg tcgcgccccg cgtgcgcctc gtacaccgtc gacgtgacgc
     3841 ccgcgcgctg caacgccagg gccgtgacag ggccggcgat gccgcccccg atgatgatcg
     3901 cgtgagtcat ggaatcctcg ttatttcttt ctctcgaacg aaatacttcg tgcccccggc
     3961 ccggtcaaga aatttccttc ggatgaacga aatacttcgt ctttgctacc ctgcgcgcga
     4021 tggctcgcaa gcggacgcgc gacaggtcgg agctgatcga ggcggtcgac gccggcgggc
     4081 gggcgctgag caccgccgcg gtgctgtttc acgccgccct cgcggcgcga cagggcctga
     4141 cgtcgaccga gaccaaggcg ctcgacctgc tggcgcgcct gggccccttg acggccgggg
     4201 cgctcggcga gcaggccggc ctcgccccgg cctcggtgac cggcctgctc gaccgcctcg
     4261 agcgcaaggg ttttgcccgc cggatccgcg accccgagga tgggcggcgc gtgctcgtcg
     4321 aggtcgtccc ggacgcgctc gccgccttcg cccccgactt cgaggatctg atgcgcgagc
     4381 ttcacgcgct gtacgaagat ttcaccgccg agcagctcga gacgatcgcc cattttttaa
     4441 atgaggccgc ccggcgtcag agcgacgcga cgatgcgcct cgccgccggg cccgtcgcgc
     4501 cgcgcaagca ggccgcgcgc cgccgcggcg attgatggcc ccgcctggag ttcgagctcg
     4561 agcccctgtg atcgagagcg gccgtctctc ggccacctca caaaagcaaa cccgaccgcc
     4621 caccggggga ggagatctcc ctcggcggca caaatttgtg cgggaatgca cgcccggggg
     4681 cgcgagactg caccgtttac cggacgccgg ttgaactcga ttcaacaacc aaaatttatg
     4741 taaggcccct tacaaaaccg gccacacatc agcgtttccg cgccacatgt gcgccgctga
     4801 aggctgacgt ctgtgtctcc aggtccatca accaaagaaa ttcctgtaag cgggcacacc
     4861 aggataaccg t
//
LOCUS       DASWNJ010001959         1745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_670781, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001959 DASWNJ010000000
VERSION     DASWNJ010001959.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670781"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VGB85_32610"
     CDS             complement(<1..639)
                     /locus_tag="VGB85_32610"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0138889.1"
                     /translation="MRKRLLSVILASSGCGEVEYGVVVDGERLPVIDMHLHPGEWSNT
                     PPDTQEFLAGRFPFPLQLTPARLAGQVLSVDGLKKELDGAGIGTGVLFAVYAPRTVGI
                     SANEDVIADVKQAPDRFFGLASLRVERWNEDEASELKRLRDALGEPGMVGVKLAHAHQ
                     HFRMDDPRYFSIYALAGELDKPVYLHTGTSPFPGTAQDPPYTDPAYLEPAITA"
     gene            898..>1745
                     /locus_tag="VGB85_32615"
     CDS             898..>1745
                     /locus_tag="VGB85_32615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138890.1"
                     /translation="MTTRSALNQRARILGERRARPRPDSRDYIHIAAHKALEAQTFNT
                     IAWQEPRSVDISGVRLIWDRLDDNYGLMVYQGEGVLDLGALTLYADQVIIRSPLRFPG
                     TTVTIHARELIFEGDGSIDTTPRRHLASKASTPLRKDGLPTYVAADGAHGEPAGDIHL
                     LIHTLRGADPGGRRRFIAVGSNGQVGEDGDYLKYKPRLTDQPEAEKGKDLNPVELDTL
                     VGHINEKFVVKTWFWPGQVREAKDMHKADGLKNDKATHVRLIARDDGLVTCNVTRTYF
                     PGKDMLG"
BASE COUNT          280 a          626 c          593 g          246 t
ORIGIN      
        1 ggcggtgatc gccggctcga gatacgccgg gtcggtgtaa ggcgggtcct gagcggtccc
       61 ggggaacggg ctggtgccgg tgtgcaggta gaccggcttg tcgagctccc cggcgagcgc
      121 gtagatcgag aagtagcgcg ggtcgtccat gcgaaagtgc tggtgcgcgt gcgccagctt
      181 gacgccgacc atgcccggct cgccgagcgc gtcgcgcaga cgtttgagct cgctcgcctc
      241 gtcctcgttc cagcgctcga ctcgcaggct cgccagaccg aagaagcggt cgggcgcctg
      301 cttcacgtcg gcgatcacgt cctcgttggc cgagatgccg accgtgcgcg gggcgtacac
      361 cgcgaacagc acgcccgtgc cgatgcccgc gccgtcgagc tccttcttga ggccgtcgac
      421 cgacagcacc tgcccggcca gccgcgccgg cgtcagctgc agcgggaacg ggaagcgccc
      481 ggccaggaac tcctgggtgt ccggcggcgt gttggaccac tcgccggggt gcaggtgcat
      541 gtcgatcacc ggcaggcgct cgccgtcgac gacgacgccg tactcgactt caccgcagcc
      601 ggagctcgcc agaatcacgg acaggagccg ctttcgcacg tcctcctcta gcatggaggc
      661 gcggcttcgc ggacctcgcc ggccgcgtga ctcatggcac ggatccaggc aggtcctgta
      721 cgatgacgag tgtctcgccg cggtgctgcg tgcggggcgt gtgcgggccg ctgtgcgccg
      781 cacatcgagc acgcggcccg ccccctcgcc cgccctgccg gcgtcggttc tcgctggcac
      841 atgccgtgca actgcgccgc gggcatgaca gctcgagcag ggaacatatc gacggcgttg
      901 accacccggt ccgcgctcaa ccagcgcgcg cgcatcctgg gcgagcggcg cgcgcgtccg
      961 cgacccgact cgcgcgacta tatccacatc gccgcccaca aggccctcga ggcgcagacc
     1021 ttcaacacga tcgcctggca ggagccgcgc agcgtcgaca tctcgggtgt gcgcctgatc
     1081 tgggaccgcc tcgacgataa ctacgggctg atggtctacc agggcgaggg tgtgctcgac
     1141 ctgggcgcgc tgacgctgta cgccgaccag gtcatcatcc gctcgccgct gcggttcccg
     1201 ggcacgaccg tgacgatcca cgcgcgcgag ctgatcttcg agggcgacgg cagcatcgac
     1261 accaccccgc gccgtcacct cgccagcaag gcctcgacgc cgctgcgcaa ggacggcctg
     1321 cccacctacg tcgcggcgga cggcgcccac ggcgagccgg ccggcgacat ccatctcctc
     1381 atccacaccc tgcgcggcgc ggacccgggc gggcgtcgtc gcttcatcgc cgtcggatcc
     1441 aacgggcagg tcggcgagga cggcgactac ctcaagtaca agccgcggct gaccgaccag
     1501 ccggaggcgg agaagggcaa ggacctcaac ccggtcgagc tcgacaccct ggtcgggcac
     1561 atcaacgaga agttcgtggt caagacatgg ttctggccgg gtcaagtccg cgaagccaag
     1621 gacatgcaca aggccgacgg cctcaagaac gacaaggcca cgcatgtgcg gctgatcgcc
     1681 cgcgacgacg gcctcgtcac ctgcaacgtg acgcgcacct acttcccggg caaggacatg
     1741 ctggg
//
LOCUS       DASWNJ010001960         4571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_417289, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001960 DASWNJ010000000
VERSION     DASWNJ010001960.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417289"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2968)
                     /locus_tag="VGB85_32620"
     CDS             complement(<1..2968)
                     /locus_tag="VGB85_32620"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0138891.1"
                     /translation="MVDADDTPPAGAPAQPATPPSYPAALNRRLATVEDTQLASGLPA
                     ASDPGLAAERTLAALPGHEPTLIGPSAAGDARPSETPRDHPARIGRYLVIGPLGEGGM
                     GLVFAAYDPQLDRKVAIKLVRPAYTESSGGEAQARLVREAQALARLRHPNIVTVYEVG
                     AFGEEVYVAMEFVDGVTLRTWQFEHARSWREILRVYQAAARGLAAAHRGGLVHRDFKP
                     DNVLVTKDGEPRVLDFGLAFSDGSKPATGAPHSAPLDAHLTLTGALLGTPAYMSPEQF
                     EGRAVDARTDVFAFAVALYEALYGARPYAGTTVAEICVALHDGRVSPPPPFVKIPGWL
                     RRAVLRGLKTDPNERLPDMDAVLRVLGRDPARYLLGASVVLVVGGIIAALVLALHSAE
                     GEQTLRDQGARARADFGHARADTLERALHARDHRAADRFNAWLVAAARERVESAPLRA
                     LAALKHLRVDGQGLSEARGVANEALGRGLPTHAWPTPAPVRTVAFASGDRHVVTGDVR
                     GQIEVHARDSGAVQASAAHDGPITALAVATRGDPIGERPPLRIAALAGNAVLLWDVAA
                     DTRRRHTVEDSAILSVALAPDGERVVTGRHDGRLQIHGWSGDLQQSYRDHTDPITAVA
                     WAGDGRWIASGGETGEVIRWQLADNTHRALGQASAAIRELHFDTMSRRLFAVAGDRGG
                     LVWSMDGDAPATPLQDLRSIRAGERVRLQLGASTTLRQDGHGERTLASDATATAIDLD
                     PSARWAAIGHADGVEIWDARARRDRSLGGTGLRLDRLLWSPDGRWIAGTAEDGSLELW
                     HVETGAHRVLRAAGPVFAAVLFNADATALIAADELPRLVSWDLRQGPPTPRELPLAAP
                     GAGAIEAEPLPDGGLLQWHAASNSWGAIQALGPDGARRWHKRDTDQILHAALSPDGAR
                     LTTAPRHGRPDFWRVEGEGLRREPIDLGGPDHRWRALASTSDGTRTRLAASIEATTRD
                     VTTGFVVWEVPWDTAVD"
     gene            complement(3054..3524)
                     /locus_tag="VGB85_32625"
     CDS             complement(3054..3524)
                     /locus_tag="VGB85_32625"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY0138892.1"
                     /translation="MSHAPRLDYRRLNPAVFKAYLAFDQAAQGSGLEHSLLELVKLRA
                     SHLNGCAYCVDMHSKDARAQGEQEQRLYALPVWREAPFFSERERAALAWTEALTAVAG
                     HGISDELFAATSKHFSETEMVALTAAVVAINGWNRFAVAFAAVAGTYQPAHKPA"
     gene            complement(3598..4167)
                     /locus_tag="VGB85_32630"
     CDS             complement(3598..4167)
                     /locus_tag="VGB85_32630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138893.1"
                     /translation="MATSRHQRDNAEAFDDLLGQLVDELALKPTMIPEPEPPPAPPPV
                     AVAPVAYQPEPAYQPPPRRDFMPLAVGGGIAVAGIAVALVLLFGGGETQPSVTERAVI
                     AARNVPPPPVQPAPPVAVATPAPAVPAAGLTGQPADPGALAQLVVPTVDSKPAVPASQ
                     PKKKVIKKVAGPAKPKPSPAKPTVVDPFG"
     gene            4166..4300
                     /locus_tag="VGB85_32635"
     CDS             4166..4300
                     /locus_tag="VGB85_32635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138894.1"
                     /translation="MVVLLKSKDARAPVVPGATCTRRLHGRGVAGINVWTPDPDLGIF
                     "
     gene            4382..>4571
                     /locus_tag="VGB85_32640"
     CDS             4382..>4571
                     /locus_tag="VGB85_32640"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY0138895.1"
                     /translation="MIRVVYRWSVVPGQEEAFIAAWRAVTLILRERVEGARGSLLLRD
                     PQAPQELTAVARWTSRDAL"
BASE COUNT          567 a         1558 c         1858 g          588 t
ORIGIN      
        1 cgtcgacggc ggtgtcccac gggacctccc agacgacgaa gccggtcgtc acgtcgcgcg
       61 tggtcgcctc gatcgacgcg gccaggcgcg tgcgcgtgcc gtcgctcgtc gacgcgagcg
      121 cccgccagcg atggtcgggc ccgccgaggt cgatcggctc gcggcgcaga ccctcgccct
      181 cgacccgcca gaagtcgggc cggccgtgac gcggcgcggt cgtcaggcgc gcaccgtcgg
      241 gcgacagcgc ggcgtgcaag atctggtcgg tgtcgcgctt gtgccagcgg cgcgcgccgt
      301 cgggcccgag cgcctggatc gccccccagc tgttgctcgc tgcgtgccac tgcaacaggc
      361 cgccgtcggg cagcggctcg gcctcgatcg cgcccgcgcc cggggccgcc agcggcagct
      421 cgcgcggcgt cggtgggccc tggcgcaggt cccaggagac gagccgcggg agctcgtcgg
      481 cggcgatcag ggcggtcgcg tcggcgttga acaggaccgc cgcgaagacc ggccctgctg
      541 cgcgcaacac ccggtgcgcg ccggtctcga cgtgccacag ctcgaggctg ccgtcctcgg
      601 cggtgccggc gatccagcgg ccgtcggggg accacagcag gcgatcgagg cgcaggccgg
      661 tgccgccgag gctgcggtcg cgacgggcgc gcgcgtccca gatctcgacg ccgtcggcgt
      721 gcccgatcgc ggcccagcgc gcgctcgggt cgaggtcgat cgcggtcgcg gtcgcgtcgc
      781 tggcgagcgt gcgctcgccg tggccgtcct ggcgcagcgt ggtactggcg ccgagctgca
      841 ggcgcacgcg ctcgccggcg cggatcgatc gcaggtcctg cagcggggtc gcgggggcgt
      901 cgccgtccat cgaccacacg aggccgccac gatcgccggc gaccgcgaac agtcggcggg
      961 acatggtgtc gaagtgcagc tcgcggatcg ccgcgctggc ctggccgagc gcgcggtggg
     1021 tattgtcggc gagctgccag cggatcacct cgccggtctc gccaccggac gcgatccagc
     1081 gcccgtcgcc ggcccacgcg accgcggtga tcgggtcggt gtgatcgcgg tagctctgct
     1141 gcagatcgcc ggaccagccg tggatctgca ggcgtccgtc gtgccggccg gtgacgacgc
     1201 gctcgccgtc gggggccagc gcgacggaga ggatggcgct gtcctcgacg gtgtgccgtc
     1261 gccgggtgtc cgcggcgacg tcccacagca gcaccgcgtt gccggcgagc gcggcgatcc
     1321 gtagcggcgg gcgctcgccg atcggatcgc cgcgggtggc gacggcgagc gcggtgatcg
     1381 ggccgtcgtg cgcagcgctg gcctgcacgg cgccgctgtc acgcgcgtgc acctcgatct
     1441 ggccgcggac gtcgccggtg acgacgtgcc gatcgccgct tgcgaaggcg acggtgcgca
     1501 ccggtgcggg ggtcggccag gcgtgggtcg ggagcccgcg gccgagcgcc tcgttggcga
     1561 cgccgcgcgc ctcggagagg ccctggccgt cgacccgcag gtgcttgagc gcggcgaggg
     1621 cccgcagcgg cgcggactcg acgcgctcgc gcgcggcggc gacgagccag gcgttgaagc
     1681 gatcggcggc ccggtgatcg cgggcgtgca gggcccgctc gagcgtgtcg gcgcgcgcgt
     1741 ggccgaagtc ggcgcgcgcg cgggcgccct ggtcgcgcag cgtctgctcg ccctcggcgg
     1801 agtgcagcgc gagcaccagc gcggcgatga tgcccccgac gacgagcacg acgctggcgc
     1861 cgagcaggta gcgcgcgggg tcgcggccga ggacccgcag gacggcgtcc atgtcgggca
     1921 ggcgctcgtt cggatcggtc ttgaggccgc gcaggacggc gcggcgcagc caccccggga
     1981 tcttgacgaa gggcgggggc gggctgacgc ggccgtcgtg cagcgcgacg cagatctcgg
     2041 cgacggtggt gccggcgtag ggacgggccc cgtagagcgc ctcgtagagg gcgaccgcga
     2101 acgcgaacac gtcggtgcgc gcgtcgacgg ctcggccctc gaactgctcc ggggacatgt
     2161 acgccggcgt gccgagcagc gcgccggtca gcgtcaggtg ggcgtcgagc ggcgccgagt
     2221 gaggagcgcc ggtcgcgggc ttcgagccgt cggagaacgc gaggccgaag tcgaggacgc
     2281 gcggctcgcc gtccttggtg acgagcacgt tgtcgggttt gaagtcgcga tggacgaggc
     2341 cgccgcggtg cgccgccgcg agcccgcgcg cggccgcttg atatacccgc aggatctcgc
     2401 gccagctgcg ggcgtgctcg aactgccagg tccgcagcgt cacgccgtcg acgaattcca
     2461 tggcgacgta gacctcctcg ccgaaggcgc cgacctcgta cacggtgacg atgttggggt
     2521 gacgcaggcg ggcgagcgcc tgggcctcgc gcacgagccg ggcctgggcc tcgccgccgc
     2581 tcgactcggt gtaggccggg cgcacgagct tgatcgcgac cttgcgatcg agctggggat
     2641 cgtaagccgc gaacacgagc cccatgccgc cctcgccgag cgggccgatg acgaggtaac
     2701 gaccgatccg ggccggatga tcgcgcggcg tctccgaggg tcgcgcgtcg ccggcggcgc
     2761 tggggccgat cagcgtcggc tcgtggcccg gcagcgctgc gagggtgcgc tccgcggcga
     2821 ggccggggtc actcgccgcc gggagaccgc tggcgagctg ggtgtcctcg acggtcgcca
     2881 ggcgccggtt gagcgccgcg gggtacgagg gcggcgtggc gggctgcgcc ggtgccccgg
     2941 cgggcggggt gtcgtcggcg tcgaccacgg tgacaggata gccaaaccgc gggtcggcgg
     3001 ggccgagtga acacgcacgg gcgcagtcgc tcgcacatcg caggcgcggc ggctcaggcg
     3061 ggcttgtgcg cgggctggta cgtgccggcg accgccgcga aggcgacggc gaagcggttc
     3121 cagccgttga tcgcgaccac cgcggccgtc agcgcgacca tctcggtctc gctgaagtgc
     3181 ttcgaggtgg cggcgaacag ctcgtcggag atgccgtgcc cggcgacggc cgtcagggcc
     3241 tcggtccagg ccagcgcggc gcgctcgcgc tcgctgaaga acggcgcctc gcgccacacc
     3301 ggcaaggcat agagccgctg ctcctgctcg ccctgggcgc gggcgtcctt gctgtgcatg
     3361 tcgacgcagt aggcgcagcc gttgaggtgc gaggcgcgga gcttcaccag ctcgagcagg
     3421 ctgtgctcga ggccggagcc ctgcgcggcc tggtcgaacg cgaggtaggc cttgaagaca
     3481 gcggggttga ggcggcggta gtcgagtcgg ggtgcgtggg acatttttca gttcctttac
     3541 acccctgacg agggccaccg ccgtcatgtg acagcatgtg acagcgagcg acagcggtca
     3601 gccgaaagga tcgacgacgg tcggcttggc cggggagggc ttgggcttgg ccgggccggc
     3661 gaccttcttg atcaccttct tcttcggctg cgacgccggc acggccggct tgctgtcgac
     3721 ggtcgggacg accagctgcg cgagcgcgcc ggggtcggcg ggctgccccg tgagacccgc
     3781 cgcgggcacg gcgggggcgg gcgtggcgac ggcgaccggc ggggccggct ggaccggcgg
     3841 gggcggcacg ttgcgcgcgg cgatcacggc gcgctcggtg acggacggct gggtctcgcc
     3901 gccgccgaac agcaggacga gggcgacggc gatgccggcg acggcgatgc caccaccgac
     3961 cgcgagcggc atgaagtccc ggcgcggcgg gggctggtac gcgggctcgg gctggtaggc
     4021 gaccggggcg acggcgaccg ggggcggcgc gggcgggggc tcgggctccg ggatcatcgt
     4081 cggcttgagg gcgagctcgt ccacgagctg accgaggagg tcatcgaagg cttcggcgtt
     4141 gtcgcgctgg tgacgggagg tggccatggt cgtgctcctg aaatccaagg acgcgcgagc
     4201 tccggtcgtg ccgggtgcga catgcacccg gcgactgcac ggtcgcggcg tcgcgggcat
     4261 caacgtgtgg acgcccgacc ccgatctagg aattttttaa tgcgccgcaa gggggacccc
     4321 gacgcggccg cgaggaacca ccagcaccta cacggagatc gcggggatgg taggctgcgc
     4381 catgatccgc gtcgtctatc gctggagcgt ggtgccgggg caggaggagg cgttcatcgc
     4441 cgcctggcgc gcggtgaccc tgatcctgcg cgagcgcgtc gagggggccc gcgggagcct
     4501 gttgctgcgt gacccgcagg cgccgcagga gctgacggcg gtcgcgcgct ggaccagccg
     4561 tgatgccctg g
//
LOCUS       DASWNJ010001961         5585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_345730, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001961 DASWNJ010000000
VERSION     DASWNJ010001961.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345730"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VGB85_32645"
     CDS             complement(<1..610)
                     /locus_tag="VGB85_32645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138896.1"
                     /translation="MSTHALIAALALVVAPVPAASPEAASPEASESDEAYAFYDQGAY
                     LEAAAVATRIYVSPAFPRATRLEHARLAQECFAQAYAKTTTTTPRPEYLCRALDVLAA
                     TAPLAINAHDVRLHETLSGVRRVTLEARHPGYTCEVGTEPRPESLPSEAPAPTASVAP
                     PDGASQPILKKDPPGSDAPVAPRTAQPRPPVSDSIPPRHLKIA"
     gene            complement(658..1476)
                     /locus_tag="VGB85_32650"
     CDS             complement(658..1476)
                     /locus_tag="VGB85_32650"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0138897.1"
                     /translation="MTTTTTDELYQAAYDRVAARYDDLFSRHVKTPNDRLTRGLGLRR
                     GERLVDLACGSGAATLPMMRLTAPAETVAVDPSEGMLSLAREAADTEGLKLTAIKAKA
                     EDFIAAAPTASFDVVSMRFALAYLDWHTVLPAMARLLRPGGRIGLVTSLATSAPQALT
                     IYHRLAESMDMETVPLPTPKTSGQVEELLTRGGVRIEDSWQHGFRLWFDTGAEATRWL
                     IDTGYVAHPALNSLDDDVLDGLVGLFGAHLENEFREDEGVPLDFFVAGIIARRP"
     gene            complement(1660..2307)
                     /locus_tag="VGB85_32655"
     CDS             complement(1660..2307)
                     /locus_tag="VGB85_32655"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEY0138898.1"
                     /translation="MPPAIDRFQAVWRFFSNAFRDWDQTASFVPSSRFLVDAIVQGAA
                     LSTARTVVELGAGTGTVTQALLEAMPADARLFAVEIDGPLLDATARRLPDPRLVAIQG
                     SAADITQLLADAGHDGPVDVVVSCLGMSLLPPELRRDILASSIAALGPNGVFVQYGYL
                     HAKVVVYSPVRGWSRFDLQAYLQDHFEQIQQQRVIANFPPADVFVCRQAVQATVH"
     gene            complement(2434..2862)
                     /locus_tag="VGB85_32660"
     CDS             complement(2434..2862)
                     /locus_tag="VGB85_32660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEY0138899.1"
                     /translation="MRTSTTHSFHSLVATLVLAASAVIPGCTCMARDAETYRADTRNL
                     LETRSGDIKSCYDAALTADPKLDGQVTVKFKVEKKTGKILDPAIDAERTKAPEALSQC
                     IIKAVDGLTLDPVDQRDGLATFSWTFKANEQQPAAEAPAA"
     gene            complement(2871..3572)
                     /locus_tag="VGB85_32665"
     CDS             complement(2871..3572)
                     /locus_tag="VGB85_32665"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0138900.1"
                     /translation="MHLSRSLLTSVTVFAALQVMACGPTLFEDATALQVVGDPPPLPA
                     PPPPPPPEPEPEPEPPKRVVVEDNRIVINEKIQFDLDKATIKPESDSLLQEIAKVVKE
                     NPHIKKIAIEGHTSSEGSDRHNLKLSDQRAKAVMDWLIKNGGLAKEMFTAKGFGETKL
                     IADETTEDGREKNRRVEFNIIEQDVTQKKVEIDPKSGAKKVLEEKHIEETAPVDEKPV
                     TTGDKAGGATTPAAK"
     gene            3927..5297
                     /locus_tag="VGB85_32670"
     CDS             3927..5297
                     /locus_tag="VGB85_32670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138901.1"
                     /translation="MFALSGLCGCDGGSQGESSTGAESTGAATDATTDGPAEACAVPT
                     AGPTVHDGELGMNEVWTAEAGPHIVTQWVRVPDGATLTIEPCAEVRMQKDAALVFGHA
                     NGTVVTTLRAEGEPDRPIRFVRDGAEPWSAIVAFDPAQLSLAHVTLEGGGSDRFLWNA
                     SLVLRGIGETPTRRLAKLVDVTIRDSVGHGVVAAWASGFLPGSSGLTVTDSGNEEFPF
                     PVRVGEHALDSLPDGDYTGNRVDEILIEDEGANLAAGLQEDATLHDRGVPYRVGEWEG
                     ARLRVGGGERPMTTLTIEPGVVLRFTPGAAFEVEHWTSDVEPATGAVVALGTAEKPIV
                     FTSAAPSPAPGDWVGLWFGSVPAAHNRIDHARIEYAGGECSCVGFTCTAAAEASVLFI
                     EGVPASGFITNTTFAHGAGHGISRGWRGGGPDFTASNRFEDIAGCAQTMTRDLNNMCP
                     DGDGCG"
BASE COUNT          820 a         1769 c         2152 g          844 t
ORIGIN      
        1 cggcgatctt caagtggcgt ggtggaatag aatcactgac agggggccgc ggctgggcgg
       61 tcctcggcgc gaccggtgcg tcgctcccag gtgggtcttt cttgaggatc ggttgggagg
      121 cgccatccgg aggcgcaacc gacgcggtcg gggccggcgc ctcggagggc aacgactccg
      181 gcctgggctc tgtccctacc tcgcacgtgt agccaggatg ccgcgcctcg agggtcaccc
      241 ggcggacccc ggagagggtc tcgtggagcc gaacgtcgtg ggcgttgatc gccagaggcg
      301 ccgtggcggc cagcacgtcg agcgcccggc agaggtattc cgggcggggc gtggtggttg
      361 tcgttttcgc gtacgcctgc gcgaagcact cctgcgccag ccgggcatgc tccagacgtg
      421 tcgcgcgagg gaaggctggc gacacgtaga tccgggtggc gacggcggcg gcctccaggt
      481 aggccccctg gtcgtagaac gcgtaggcct catcggattc gctggcctcc ggcgaagcgg
      541 cctccggcga agctgcgggc accggcgcca cgactagagc cagggctgcg atcaaggcat
      601 gcgtggacaa gcagccacag gactacccgc gcgaacgcgc gcagtcaaga tcgcgcatca
      661 ggggcggcgg gcgatgatgc cggcgacgaa gaagtcgagc gggacgccct cgtcctcgcg
      721 aaactcgttc tcgaggtggg cgccgaacag gccgacgagg ccgtcgagca cgtcgtcgtc
      781 gaggctgttg agggccgggt gggcgacgta gccggtgtcg atcagccagc gggtcgcctc
      841 ggcgccggtg tcgaaccaca ggcgaaagcc gtgctgccag ctgtcctcga tgcgcacgcc
      901 gccgcgggtc agcagctcct cgacctggcc gctggtcttg ggcgtcggca gcgggacggt
      961 ctccatgtcc atgctctcgg cgaggcggtg gtagatcgtc agcgcctgcg gggcgctggt
     1021 ggcgagcgag gtcaccaggc cgatgcggcc gccaggacgc aggagacggg ccatcgccgg
     1081 cagcaccgtg tgccagtcga ggtaggcgag cgcgaagcgc atcgagacga catcgaagga
     1141 cgcggtcggg gcggcggcga tgaagtcctc ggccttggct ttgatcgcgg tcagcttgag
     1201 gccctcggtg tcggcggcct cgcgggcgag gctcagcatg ccctcggagg ggtcgaccgc
     1261 gacggtctcg gccggtgcgg tcagccgcat catcggcaag gtcgccgcac cgctgccgca
     1321 ggcgaggtcg acgaggcgct cgccgcgacg cagaccgagg cctcgcgtga ggcgatcgtt
     1381 cggggtcttg acgtggcggc taaacaggtc gtcgtagcgc gcggcgacgc ggtcgtaggc
     1441 ggcctggtag agttcgtcgg tggtcgtcgt ggtcaacgaa ggtctccggt cgggggcgaa
     1501 tcgggggacc gcgccgcagt cgcggcggaa tcgcgaaatc gatacaccga gccggccgac
     1561 cgcgcaacgt cgcgaccgaa ccgggacctg cgacgggctt tatggagcgg ccggtgagaa
     1621 ccctcgggtg tgcagtcacc cgcacatcgc ccgcgaacct cagtgcacag tcgcctgaac
     1681 ggcctgacgg cacacgaaca cgtcggcggg cggaaagttg gcgatcaccc gctgctgctg
     1741 gatctgctcg aaatggtcct gcaggtaggc ttgcaggtcg aagcgcgacc agccgcggac
     1801 cgggctgtag acgacgacct tggcgtgcag gtagccgtat tgcacaaata cgccgttcgg
     1861 gccgagggcg gcgatcgagc tggcgaggat gtcccggcgc agctcgggcg gcagcaggga
     1921 catgccgagg caggacacga ccacgtcgac cgggccgtcg tggccggcgt cggcgagcag
     1981 ctgggtgatg tcggcggccg agccctggat cgccaccagg cgcgggtcgg gcaggcgcct
     2041 cgcggtcgcg tcgagcagcg ggccgtcgat ctcgacggcg aacaggcgag cgtccgcggg
     2101 catcgcctcg agcagcgcct gggtgaccgt gccggtgccg gctccgagct cgacgacggt
     2161 gcgcgccgtc gacagcgcgg cgccctggac gatcgcatcg acgaggaagc gcgagctggg
     2221 gacgaacgag gcggtctggt cccagtcgcg aaacgcgttg gagaagaacc gccaaacagc
     2281 ctggaaacga tcgatcgcgg gaggcacggg cggccgcgag tgtaacgaga tcccgccgaa
     2341 tgtcacgttc ggcgggatct cgcggtcagc cagcgtttgt ggtgctggcc gggtgtgcgc
     2401 gtgcctgcac gcaggctggc acgcgcgacg cgctcaggcc gcgggggcct cggcggcggg
     2461 ctgctgctcg ttggccttga aggtccagct gaaggtcgcg aggccgtcgc gctggtcgac
     2521 cgggtcgagg gtgaggccgt cgaccgcctt gatgatgcac tggctcaggg cctcgggggc
     2581 cttggtgcgc tcggcgtcga tcgccgggtc gaggatcttg ccggtcttct tctcgacctt
     2641 gaacttgacg gtcacctgac cgtcgagctt ggggtcggcg gtgagggccg cgtcgtagca
     2701 gctcttgatg tcgccgctgc gggtctcgag caggttgcgg gtgtcggcgc ggtaggtctc
     2761 cgcgtcgcgg gccatgcagg tgcagccggg gatcacggcg gaggctgcca ggacgagagt
     2821 ggcgacgagc gagtggaagc tgtgagtggt gctggtacgc atggtgggtc ctacttcgcg
     2881 gccggggtgg tggcgccgcc ggccttgtcg ccggtggtca cgggcttctc gtcgacgggc
     2941 gccgtctcct cgatgtgctt ctcctcgagc accttcttcg cgccggactt cgggtcgatc
     3001 tcgaccttct tctgggtgac gtcctgctcg atgatgttga actcgacgcg gcggttcttc
     3061 tcgcggccgt cctcggtggt ctcgtcggcg atcagcttgg tctcgccgaa gcccttggcc
     3121 gtgaacatct ccttggccag cccgccgttc ttgatcagcc agtccatgac cgccttggcg
     3181 cgctggtcgg agagcttgag gttgtggcgg tcggagccct cgctcgaggt gtggccctcg
     3241 atcgcgatct tcttgatatg cgggttctcc ttgacgacct tggcgatctc ttgcagcagg
     3301 gagtcggact ccggcttgat cgtcgccttg tcgaggtcga actggatctt ctcgttgatg
     3361 acgatgcggt tgtcctcgac gacgacgcgc ttgggcggct cgggctcggg ctcgggctcg
     3421 ggcggcggcg gaggcggcgg cgccggcaac ggcggcgggt cacccacgac ctggagggct
     3481 gtggcgtcct cgaacagggt gggaccgcag gccataacct ggagcgccgc gaacacggtc
     3541 acggaggtga gaagggagcg ggagagatgc atgagtggtc tcgatggctg gggtgatggt
     3601 cccgggcttt tcagcccaaa accgagcggt atcgcgctga tctgcggttg cccaagggtc
     3661 gcccagcagg gcaggcggcg caagtcctcg actggcgccg ccgcgaaggt gaccccgaag
     3721 cggcaattga gcaaggaagg ctcacattcg tgggcgcgcg gtggcaatgg gagatttaag
     3781 tcgcggcccg gcgaccccgg ctcgtgatcc gtcgcggggg gtcgccacgc caatggacgc
     3841 cgttgaaggg gccgtggggg ccccggggcg ccaggagtgg gaccgtgaag acgcttgttt
     3901 ggcaaacttg gtcgatcgtg ggagcgatgt tcgccctgtc ggggctgtgc ggttgtgatg
     3961 ggggttcgca gggcgagagc tcgaccggtg cggagagcac cggggccgcc accgatgcca
     4021 ccaccgatgg gcccgccgag gcctgtgccg tgccgacagc ggggccgacc gtgcacgacg
     4081 gggagctcgg gatgaacgag gtctggacgg cggaggccgg cccacatatc gtcacgcagt
     4141 gggtcagggt cccggacggc gcgacgctga cgatcgagcc gtgcgcggag gtgcggatgc
     4201 aaaaggacgc cgcgctggtg ttcgggcacg ccaatggcac ggtggtgacg acgctgcggg
     4261 ccgagggcga gcccgaccgc ccgatccgct tcgtgcgtga cggcgccgag ccgtggagcg
     4321 cgatcgtggc cttcgacccc gcgcagctgt cgctggcaca cgtgacgctg gagggcggcg
     4381 gctcggatcg cttcctgtgg aacgcctcgc tggtgctgcg cgggatcggt gagacgccga
     4441 cgcgccggct ggcgaagctc gtcgacgtga cgatccgcga ttcggtcggg cacggcgtgg
     4501 tcgcggcgtg ggcctcgggc ttcttgccgg gatcgtccgg gctgaccgtc accgacagcg
     4561 gcaacgagga gttcccgttc ccggtgcggg tcggcgagca cgcgctcgac tcgctgccgg
     4621 acggcgacta caccggcaac cgcgtcgacg agatcctgat cgaagacgag ggcgccaacc
     4681 tggccgcggg cctgcaagag gacgcgacgc tgcacgaccg cggcgtgccg taccgcgtcg
     4741 gcgagtggga gggcgcgcgg ctgcgagtcg gcggcggcga gcggccgatg acgacgctga
     4801 cgatcgagcc cggcgtggtg ctgcgcttca cgcccggcgc ggccttcgag gtcgagcact
     4861 ggaccagcga cgtcgagccg gcgaccggcg ccgtggtggc cctcggcacg gcggagaagc
     4921 cgatcgtgtt cacctcggcc gcgccgagcc ccgcgcccgg cgactgggtc gggctgtggt
     4981 tcggcagcgt gccggcggcg cacaaccgca tcgaccacgc gcgcatcgag tacgccggtg
     5041 gcgagtgcag ctgcgtcggc ttcacctgca cggccgccgc cgaggcgagc gtgctgttca
     5101 tcgagggcgt gccggccagc ggcttcatca ccaacacgac cttcgcccac ggggcgggcc
     5161 atggcatcag ccgcggctgg cgcgggggcg gcccggactt caccgcgagc aaccgcttcg
     5221 aggacatcgc cggttgcgcg cagacgatga cgcgcgacct caacaacatg tgccccgacg
     5281 gcgacggctg cggctgagac gcggcgaccg cgagctgcga agctcatgcg gtcgagatct
     5341 cgggaccacc gaggtcggga cgaccgggtc cagggagccg ctggcgaact cacagggaga
     5401 tccctcgcgg catcctggtc gcgaagatcg cccaagcgaa atgaagatcg cttaaatgaa
     5461 aatacccggg cccaagggcc cgggtattgg tcagcagatt gctggaggct cagtagccgc
     5521 cgcgaccgcc acgaccaccg ccgccgccgc cgccgcgacc accgccgccg ccgccgccgc
     5581 cgcga
//
LOCUS       DASWNJ010001962         6952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_417315, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001962 DASWNJ010000000
VERSION     DASWNJ010001962.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6952
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417315"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VGB85_32675"
     CDS             complement(<1..475)
                     /locus_tag="VGB85_32675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEY0138902.1"
                     /translation="MLKRRIIPCLDIKNGRVVKGVHFAGLRDTGDPVEAARAYDLQGA
                     DEICLLDITATVEGRETFIDVVHRVADQIFTPLTVGGGVRREADVRRLLKAGADKVAI
                     NSAAVANPLLIRQLADKYGSQCIVIAVDVKQMKPMVGDTEPLYEVVIHGGTRSTGL"
     gene            complement(469..1215)
                     /locus_tag="VGB85_32680"
     CDS             complement(469..1215)
                     /locus_tag="VGB85_32680"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_function: GO:0016860 - intramolecular oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HEY0138903.1"
                     /translation="MQGTTRMTGMLAIPAIDLLEGRAVRLSEGDPARATVYAADPRAK
                     LREFVHAGAKRIHVVDLDGAFAGRPVQTELVRSLVADAHALGAEVEVGGGLRDRAAVE
                     AALATGADYAVIGTLAVREPDIAHALCRAYPGRVIVAIDARDGWVAIAGWQELSRVPA
                     AALARAAASWGAVALLYTDVARDGLQVGAAVESTAALQRGVEIPVIASGGVGSLADLD
                     ALRRQNIHAVILGRALYEGSFTLEEALARC"
     gene            complement(1269..1889)
                     /gene="hisH"
                     /locus_tag="VGB85_32685"
     CDS             complement(1269..1889)
                     /gene="hisH"
                     /locus_tag="VGB85_32685"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEY0138904.1"
                     /translation="MPREVILVDSQLGNLDSVEHALIQVGASVHITSDPDRVATARTV
                     VFPGQGSFGPTTQAIVDGAMGAALRMVVERGDPFLGICLGMQMLFDSSDEAGGHAGLG
                     VFPGQVKRFPSDMPAGEQPGARMLKIPHVGWNDVQPTNEHFYFTHSYYVIPGNPEDVM
                     WTCRYGRVEFPAAVARGNVFGCQFHPEKSQWSGLRFLRSFILGGRG"
     gene            2164..3144
                     /locus_tag="VGB85_32690"
     CDS             2164..3144
                     /locus_tag="VGB85_32690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138905.1"
                     /translation="MGRPLGATQGPRVAVVVALRGDVKVETAGEGMSFTAKPELQLLR
                     DDRLVVGPDSFVVVALYNGHMVRLNAGTDLRVDATATFHDPPAGADLEESFAQLLTPS
                     ERDDVALRGAVTRVAGWNTRMSAAETIAPQAPPTPSLLEAPVRAEEDAEQIEAGAPPP
                     SAAKPAGYSAADQPMDGLGGGGSAEPPQQRTTPAKDDAPATDTMPGAKKAKAPNKPES
                     DKDEGRDAPSSGASSKPGPTTTLDLPKIVVHQADRDGKRTNVSLPGPFVPVRAELAAC
                     AGAGAKIRGQISGKKLVELQVNGAAKKCVPGLIGKAVALEDGWIEMTVTP"
     gene            3141..5348
                     /locus_tag="VGB85_32695"
     CDS             3141..5348
                     /locus_tag="VGB85_32695"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY0138906.1"
                     /translation="MSEALRRWLRSPARIGLVLACLCAALCAMHRAGRGIAFLERLER
                     ATLDLRFRWRGPVAPGDAAVIVALDDRTLAEAPELIERREGAARLIAAVAGHGPAAIG
                     IDAFYTDEERLLAPDLVADIRTYLTDRPGEDPATALLRRVEAETGGDAKLVDAIAAAG
                     KVVLAMHLANDRDPLPKDPALARGRYGQEVIGAHRPAAASTGVLSLPRFNAAARALGV
                     VTIYEDDDHTVREMLFARRVDDAVYMPFVVPLLALQQGISPAETAYVSDARRVQIGGL
                     RVPLTAWDSLLLNFRGPAGSFPQVSAADVIAGRVDPALLRGKIALIGVTYFGHDTTRT
                     PFGAGVPGVELHATAIDTVLAGDALVRASALVETLGCLLSGLLVALVFVARLRLGAVA
                     RIAGVLAVAAADVGLAWGLFLGAHRWHGVVWPLICVALVGSVGLAMAYVGEAVQRVRL
                     RRTFAHYLGAEVLEELLADPHLMDLGGARRELTVLFSDIRDFTGLSERLTPEELVALL
                     NVYLTPMTEEVLVRGGYLDKYIGDAVMAVYGAPVPKVDHAARALATATAMRVALAKLE
                     PTLQARGIELRIGVGINTGDMVVGNMGSRERFDYTVVGDAVNLASRLEGLTKVYGVFC
                     IVGPHTRAAAPAYRFRELDLVRVKGKGQPVAIHELLGGPDGDVAAYHELGRWDAVLAA
                     FRGGDLDVARRELAGFAAANPGDPVAAIYEQRLAALGDVVPPGWDGVMIFNSK"
     gene            5474..>6952
                     /locus_tag="VGB85_32700"
     CDS             5474..>6952
                     /locus_tag="VGB85_32700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138907.1"
                     /translation="MTQRTRGSRWGLVAVALAWSVHAEAVAQEARPADKPAERSARPS
                     VRVPARNPAGAAKALAALARFASGQLTESPYDRRVAAALDRLPGAKATAARIVAAYTS
                     MTPDDRGVVFPGVAPDQLLTRGFDRVGFDRWRAAHAEANAAKLGARTTLPPDPADGKN
                     RAQYELVYRGMKVHKGADADGTDEPVVFTAVFTPGGPTEPYRAVARTLPDSGTLGVAN
                     GASSGASAGEVWSSPSFPGGWNSGLVIVTAVIEDNGDLAQRKEDLNLLLALAKSEASE
                     DNNPDRMLVLRRELEDTLELLHLADRQTWDANAVQVRLLTASEYDALSTQAAQASPFP
                     HKLKIDHAPRGGEYTLYFDVPKPQSPRKTVQITIKQLEALGPERDRFENQQADFGVDV
                     SASASTQAGVSRTFARNKNVLKAGWTVERQVQAGGNVAISLRLWDRDPPPRTACTSQA
                     GWPHALCHSYCAATPVACTPNQANQNHCPAYDGACPDAQLDYD"
BASE COUNT          976 a         2429 c         2625 g          922 t
ORIGIN      
        1 ccaggccggt cgaccgggtg cctccgtgga tgacgacctc gtacagcggc tcggtgtcgc
       61 cgaccatggg cttcatctgc ttgacgtcga cggcgatgac gatgcactgg ctgccgtact
      121 tgtcggcgag ctggcggatc agcagcgggt tggcgaccgc ggcggagttg atcgcgacct
      181 tgtcggcgcc ggccttgagc aggcggcgga cgtcggcctc gcggcgcacg ccgccgccga
      241 ccgtcagcgg cgtgaagatc tggtcggcga cacggtgcac gacatcaatg aaagtctcgc
      301 ggccctcgac ggtcgccgtg atgtcgagca ggcagatctc gtcggcgccc tggaggtcgt
      361 aggcgcgggc ggcctcgacc gggtcgccgg tgtcacgcag gcccgcgaag tggacgccct
      421 tgacgacgcg gccgttcttg atgtccaggc aggggatgat gcggcgcttt aacatcgggc
      481 gagggcctcc tccagcgtga agctgccctc gtacagggcc cgccccagga tcacggcgtg
      541 gatgttctgc cggcgcaggg cgtcgaggtc ggcgaggctg ccgacgccac cgctggcgat
      601 cacggggatc tcgacgccgc gctgcagggc cgcggtcgac tcgacggcgg cgccgacttg
      661 caggccgtcg cgggcgacat ccgtgtacag cagggcgacc gcgccccacg aggcggccgc
      721 gcgggcgagc gccgccgcgg gcacgcgcga cagctcctgc cagccggcga tcgcgaccca
      781 gccgtcgcgc gcgtcgatcg cgacgatgac gcggcccggg taggcgcggc acagcgcgtg
      841 ggcgatgtcc ggctcgcgca cggcgagcgt gccgatcacg gcgtagtcgg ccccggtggc
      901 gagcgcggcc tcgacggcgg cgcggtcacg caggccgccg ccgacctcga cctcggcccc
      961 gagcgcgtgg gcgtcggcga cgagcgagcg cacgagctcg gtctgcacgg gtcggccggc
     1021 gaaggcgccg tcgaggtcga cgacgtggat ccgcttggcg ccggcgtgga caaactcgcg
     1081 cagcttcgcg cgcgggtcgg cggcgtagac ggtggcgcgc gcggggtcgc cctccgacag
     1141 tcgcacggcc cgaccctcga ggagatcgat cgccgggatc gccagcatgc cggtcatgcg
     1201 cgtggtgccc tgcatcgggg tcgtccccaa agaccgcggc aaagagcggg ggaaagagcg
     1261 ggggaggctc acccgcggcc tccgaggatg aagctgcgga ggaagcggag ccccgaccac
     1321 tgcgacttct ccgggtggaa ctgacagccg aacacgttgc cgcgagcgac ggcggccggg
     1381 aactcgacgc gcccgtagcg gcaggtccac atcacgtcct cggggttgcc ggggatgacg
     1441 tagtagctgt gcgtgaagta gaagtgctcg ttcgtcggct gtacatcgtt ccagccgaca
     1501 tgggggatct tgagcatgcg cgcgccgggc tgctcgccgg ccggcatgtc ggacgggaag
     1561 cgcttgacct gcccggggaa cacgccgagg cccgcgtggc cgccggcctc gtcggaggag
     1621 tcgaacagca tctgcatgcc gaggcagatg ccgaggaacg ggtcgccgcg ctcgacgacc
     1681 atgcgcaggg ccgcgcccat cgccccgtcg acgatcgctt gcgtggtcgg gccgaatgat
     1741 ccctggccgg gaaacacgac cgtgcgcgcc gtggcgaccc ggtccgggtc gctcgtgatg
     1801 tgcaccgaag cgccgacctg gatgagcgcg tgctcgacgc tgtccaggtt cccgagctga
     1861 ctgtccacca ggatgacttc gcgcggcatc ggagcggggg ccgttttacc acaggttttg
     1921 gcccaagatg gcccagtttt tttgccacgt gccacagctg cgggccaacg gctgcgggga
     1981 tgggcgggga tgggcgggcg ggctctggcg agccttgtgg taaaccttcg agccgtcaag
     2041 gggcgctgga gggccatcat gcgcgtttta tcgaggttgg cagggtggat gctcggcgct
     2101 gcgttggcga tcggttgtac cggcacgggt gagccggccg ggcccgggca aggcctgccg
     2161 gcggtgggcc ggcctctcgg ggccacgcag gggccgcggg tggcggtcgt cgtggcgctt
     2221 cgcggcgacg tgaaggtcga gaccgccggc gagggcatga gcttcacggc gaagcccgag
     2281 ctgcagctgc tgcgtgacga ccggctcgtc gtcgggcccg acagcttcgt ggtcgtcgcg
     2341 ctgtacaacg gccacatggt ccgcctcaac gcggggacgg acctgcgcgt cgacgcgacg
     2401 gcgacttttc atgatccgcc ggccggcgcc gacctcgagg agagcttcgc gcagctgctg
     2461 acgccgtccg aacgcgacga cgtcgcgctg cgcggcgcgg tcacccgcgt cgccggctgg
     2521 aacacgcgca tgagcgccgc ggagacgatc gcgccgcaag ccccaccgac gccctcgctt
     2581 ctggaggcgc cggtgagggc cgaggaggac gcggagcaga tcgaggccgg ggcgccgccg
     2641 ccgagcgccg cgaagcccgc gggctacagc gcggcggacc aaccgatgga cggcctcggc
     2701 ggtggtggat ccgcggagcc tccgcagcag cgcacgacgc ctgccaagga cgacgcaccc
     2761 gccacggaca cgatgcccgg cgcgaagaag gcgaaggcgc cgaacaagcc ggagtccgac
     2821 aaggacgagg gcagggatgc gccgtcgtcg ggcgcctcgt cgaagccggg accgacgacg
     2881 acgctcgacc tgccgaagat cgtcgtgcat caggccgacc gcgacggcaa gcgcaccaac
     2941 gtcagcttgc ccgggccgtt cgtgccggtg cgcgccgagc tggccgcgtg cgctggcgcg
     3001 ggcgcgaaga tccgcgggca gatctccggc aagaagctcg tcgagctgca agtgaacggg
     3061 gcggcgaaga agtgcgtgcc gggcctgatc ggcaaggcgg tcgcgctcga ggacggctgg
     3121 atcgagatga ccgtgacgcc gtgagcgagg ccctgcgccg ctggttgcgc tcgccggccc
     3181 gcatcgggct ggtgctcgcg tgcctgtgcg ccgcgctgtg tgcgatgcac cgcgccgggc
     3241 gggggatcgc gttcctcgag cggctcgagc gggcgaccct cgacctgcgc tttcgctggc
     3301 gcgggccggt cgcgccgggc gacgccgcgg tgatcgtcgc gctcgacgac cgcacgctcg
     3361 cggaggcgcc ggagctgatc gagcggcgcg agggcgcggc ccggctgatc gcggcggtcg
     3421 ccgggcacgg gccggcggcg atcggcatcg acgcgtttta taccgacgag gagcgcctgc
     3481 tcgcgccgga cctcgtcgcc gacatccgca cctacctgac cgatcggcca ggcgaagatc
     3541 ccgcgacggc gctgctgcgg cgcgtcgagg ccgagaccgg cggcgacgcg aagctggtcg
     3601 acgcgatcgc ggcggccggc aaggtcgtgc tggcgatgca cctcgcgaac gaccgcgacc
     3661 ccttaccaaa agatcccgcg ctggcccgcg gacgctacgg gcaggaggtg atcggcgcgc
     3721 accggcccgc ggcggcgagc acgggggtgc tctcgctgcc gcgcttcaac gcggccgcgc
     3781 gcgcgctcgg ggtcgtcacg atctacgagg acgacgacca caccgtgcgc gagatgttgt
     3841 tcgcgcgacg ggtcgacgac gccgtctaca tgccgttcgt ggttccgttg ctggcgctgc
     3901 agcagggcat atcgccggcc gagaccgcgt acgtgtccga cgcgcggcgg gtccagatcg
     3961 gcggtttgcg ggtgccgttg accgcgtggg acagcctgtt gctcaacttc cgcgggccgg
     4021 cgggcagctt cccgcaggtc tcggccgccg acgtgatcgc ggggcgggtc gacccggcgc
     4081 tgttacgcgg gaagatcgcg ctgatcggtg tgacctactt cgggcacgac accacccgta
     4141 cgccgtttgg cgcgggggtg ccgggggtcg agctgcacgc gaccgcgatc gacacggtcc
     4201 tcgccggtga cgccctggtg cgcgcgtcgg ccctcgtcga gacgctcggg tgcctgctgt
     4261 cgggcctgct ggtcgcgctg gtgttcgtcg cgcggctccg gctcggggcc gtggcgcgca
     4321 tcgccggggt gctcgccgtc gccgcggcgg acgtcgggct cgcgtggggg ctgttcctcg
     4381 gcgcacaccg ctggcacggg gtcgtctggc cgctgatctg cgtcgcgctg gtcggcagcg
     4441 tcgggctggc gatggcctac gtcggcgagg cggtccagcg cgtgcgcctg cgccggacct
     4501 tcgcgcacta cctcggcgcc gaggtgctcg aggagctgct cgccgacccg cacctgatgg
     4561 acctcggcgg cgcgcgccgg gagctgacgg tgctgttctc cgacatccgc gacttcaccg
     4621 ggctgtccga gcggctgacg cccgaggagc tggtcgcgct gctcaacgtg tacctgacgc
     4681 cgatgaccga ggaggtgctc gtgcgcggcg gctacctcga caagtacatc ggcgacgccg
     4741 tgatggcggt atacggcgcc ccggtgccca aggtcgacca cgccgcgcgg gcgctggcga
     4801 cggcgacggc gatgcgcgtc gcgctggcga agctggagcc gaccttgcag gcgcggggca
     4861 tcgagctgcg catcggcgtc ggcatcaaca ccggtgacat ggtggtcggc aacatgggct
     4921 cgcgcgagcg cttcgactac acggtcgtcg gcgacgcggt gaacctcgcc tcgcggctcg
     4981 aggggctgac caaggtatac ggggtgttct gcatcgtcgg cccgcatacg cgggcggcgg
     5041 cgcctgcgta ccggtttcgc gagctcgacc tggtgcgcgt caagggcaag gggcagccgg
     5101 tcgcgatcca cgagctgctc ggcggccccg acggtgacgt cgccgcgtac cacgagctcg
     5161 gccgctggga cgcggtgctg gctgcgtttc gcgggggtga cctcgatgtc gcgcggcgcg
     5221 agctcgcggg cttcgccgcg gccaaccccg gcgacccggt cgcggcgatc tacgagcagc
     5281 gactcgccgc gctcggcgac gtcgtgccgc cgggctggga cggcgtcatg atcttcaata
     5341 gcaagtgatg gcggcgcgcc cgcgaccctc gcgcggtcct gacgcgtcgc gttccggcga
     5401 cgacacgcgc agaactatga gacaaactga agctgacgtt ctcggcgcct gggacgccgg
     5461 caggagaaga ccgatgacgc agcggactcg aggatctcgg tggggcctgg tcgccgtggc
     5521 gctcgcgtgg agcgtgcatg ccgaggccgt cgcgcaggag gcccgacccg ccgacaagcc
     5581 cgccgagagg tccgcgcgcc cgtccgtccg cgtgcccgcc cgcaaccccg ccggcgccgc
     5641 caaggccctc gccgcgctgg cccgcttcgc cagcggccag ttgaccgagt ccccctacga
     5701 ccgtcgcgtc gccgccgcgc tcgaccggct gcccggcgcg aaggcgaccg cggcgcgcat
     5761 cgtcgccgcg tatacatcga tgacgcccga cgaccgcggc gtggtgttcc cgggcgtcgc
     5821 gcccgaccag ctgctgaccc gcggcttcga ccgcgtcggc ttcgacaggt ggcgggccgc
     5881 gcacgccgag gcgaacgccg cgaagctcgg cgccaggacc acgctgccgc ccgaccccgc
     5941 cgatggcaag aaccgggccc agtacgagct ggtgtaccgc ggcatgaagg tgcacaaggg
     6001 cgccgacgcc gacggcaccg acgagccggt cgtgttcacg gccgtgttca cgccgggcgg
     6061 tccgaccgag ccgtaccgtg cggtcgcgcg gacgctgccc gacagcggca cgctcggcgt
     6121 cgccaacggc gcgagctcgg gcgccagcgc gggcgaggtg tggtcgagcc cgtcgttccc
     6181 gggcgggtgg aacagcggcc tggtgatcgt gaccgccgtg atcgaggaca acggcgacct
     6241 cgcgcagcgc aaggaggacc tcaacctgct gctcgcgctg gcgaagtccg aggcgagcga
     6301 ggacaacaac cccgaccgca tgctcgtgct gcggcgcgag ctcgaggaca ccctcgagct
     6361 gctgcacctc gcggaccgcc agacctggga cgcgaacgcg gtccaggtgc gcctgttgac
     6421 cgccagcgag tacgacgcgc tgtccaccca ggcggcgcag gcgagcccct tcccgcacaa
     6481 gctcaagatc gaccacgccc cgcgcggcgg cgagtacacg ctctacttcg acgtcccgaa
     6541 gccgcagagc ccgcgcaaga ccgtgcagat cacgatcaag cagctcgagg cgctcggccc
     6601 cgagcgcgac cgcttcgaga accagcaggc cgacttcggc gtcgacgtgt ccgccagcgc
     6661 cagcacccag gcgggcgtgt cacgcacctt tgcccgcaac aagaacgtgc taaaggccgg
     6721 ctggaccgtc gagcgccagg tccaggccgg cggcaacgtg gcgatctccc tgcgcctgtg
     6781 ggaccgcgac ccgccgccga ggaccgcgtg caccagccag gccggctggc cgcacgcgct
     6841 gtgtcacagc tactgcgcgg cgacgccggt cgcgtgcacg ccgaaccagg ccaatcagaa
     6901 ccactgcccg gcgtacgacg gcgcctgccc ggacgcccag ctcgactacg ac
//
LOCUS       DASWNJ010001963         4037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_246542, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001963 DASWNJ010000000
VERSION     DASWNJ010001963.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246542"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1998
                     /locus_tag="VGB85_32705"
     CDS             <1..1998
                     /locus_tag="VGB85_32705"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY0138908.1"
                     /translation="DLEQLLARFADESWRERKQAVKDLQATLVRRTPPPAVFEALLER
                     LLDGLTAESVPARSTCHEMLVHLGPRGLDGALRRLISGDRGARMYVDLLADIGAPEHV
                     PLLLGVLRETSDDNVRASAAAALGAIGGPVAEEALREVLAGSSEMLQLFALDALRTIG
                     AALPVEELIPLVENDVTRRAATSLLGHSATPEALDLLVALLRDSMAGVRAAAAVALVT
                     LDAALAEQGRPGAVASAVSGSIDDSVRDRLRDLIEHRQTEVRIAAIELCRMIRDTGAL
                     RHVLSVMSDPIVRERAAGFVAGLAAQAGPALEQLAASVPPNRQGDLYALIGAVGPGVS
                     PALIALLVGGLGHRDPATAGVAAGALGRVGDASSLGPLAAALAAPDPLGEAAAAALVA
                     LIDRHGPGTPGVQALLRGAGSATGTAACNLCRVVGALGRGGDPVFVQPLVTLLGAPDP
                     LVRVAAARALGQIGGEHEGVTALTMALGDTDPGVRASACRSLGKLGAGEACQALLAVT
                     TDDSAQVRAAAVQALVSLDNPVGLARLREIVLSDNAAIVVIPAIAGLGRSGGDLDLTL
                     LMSLCGSSDHEVAKAAARGLVRFPAHRATAAVLGLLAHDRWDVRWAAAEVLAERGDLT
                     ALEPLRRVQRQETDPLVRQVVASAVARLEGLAAASGSESPP"
     gene            1995..2876
                     /locus_tag="VGB85_32710"
     CDS             1995..2876
                     /locus_tag="VGB85_32710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEY0138909.1"
                     /translation="MSRPDPSSIGGVRVRISAEETRLLQELILEHCGVSLGADAGFLF
                     ERRLGPRLDTLELGSFLEYVHHLSYDLDGPAELEECVERITTHETYFFREQFQLDAFT
                     REILPQLRAGGPRRDGTGPGPSVWSAGCSTGEEAYTIAMLLTEAGGITAAAAGQVVGS
                     DISRKVLATAQQGVYGAASFRTTADELRDRRFEPAGAGLWRVAPDVRRMCSFVHLNLM
                     DVSRFAALGRHDVIFCRNVLMYLSSQARHRIVEGFYDALVPGGFLLLGHSESLLNVTT
                     RFDLVQLSGDLVYRRPT"
     gene            2909..>4037
                     /gene="cheB"
                     /locus_tag="VGB85_32715"
     CDS             2909..>4037
                     /gene="cheB"
                     /locus_tag="VGB85_32715"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HEY0138910.1"
                     /translation="METPDPPQRAAGQKVRVLVVDDSAYNRQTISAMLESDPMVEVIG
                     RASNGKEALRQAFDLLPDVITLDLEMPEMDGFAFLRLLMAKRPLPVIIISSHSERDNV
                     FRALELGAMDFISKPGRHIGPDLRAIQEDLLAKIGMVRRLSVVRLVARAQALVEQRLE
                     RTTGELPAYESGRHDANLAASNQMVGVLGIAASTGGPPAIQQLLCALPPTLPLAILVA
                     QHMPARFTRAFAARLDKICAFQVVEASDAQVLRAGVVYIAPGAANLEVEIAGPGGARV
                     RVRPPRAGASIVPSGDLLFKTAGDSFGPRFCALILTGMGSDGREGAELARRVGATVLA
                     EDPRTAIMPGMPGSAIDAGVVDQVLALEELPVAIQRFADRCR"
BASE COUNT          487 a         1611 c         1382 g          557 t
ORIGIN      
        1 gacctcgagc aactgctcgc ccgcttcgcc gacgagagct ggcgcgagcg caagcaagcc
       61 gtcaaggacc tccaggcgac gctcgtccgg cgcacgccgc cgccggccgt gttcgaggcg
      121 ctgctcgagc gcctgctcga cggcctgacc gcggagtccg tgccggcgcg atcgacctgc
      181 cacgagatgc tcgtgcacct cggcccccgc ggcctcgacg gcgcgctgcg ccgcctgatc
      241 tccggcgacc gcggggcccg gatgtacgtc gacctgctcg ccgacatcgg cgccccggag
      301 cacgtcccgc tgctgctcgg ggtactccgc gagaccagcg acgacaacgt gcgcgcctcg
      361 gcggccgcgg ccctcggggc gatcggcggg cccgtggccg aggaggcgct gcgcgaggtc
      421 ctcgccggca gctcggagat gctgcagctg ttcgccctcg atgcactgcg cacgatcggc
      481 gccgcgctcc ctgtggagga gctgatcccg ctcgtcgaga acgacgtcac ccggcgcgcc
      541 gcgacctcgc tgctcggcca tagcgcgacc cccgaggcgc tcgacctgct cgtcgccctg
      601 ctgcgcgaca gcatggccgg cgtgcgcgcg gccgcggcgg tcgccctcgt caccctcgac
      661 gccgcgctcg ccgagcaggg ccgccccggc gccgtcgcct cggcggtgtc cggcagcatc
      721 gacgactccg tgcgcgaccg cctgcgcgac ctgatcgagc accgccagac ggaagtccgg
      781 atcgccgcga tcgagctgtg tcgcatgatc cgtgacacgg gcgccttgcg acatgtcctc
      841 tcggtcatgt cggatccgat cgtgcgcgag cgggcggccg gcttcgtcgc cggcctcgcc
      901 gcgcaggccg gacccgcgct cgagcagctc gccgccagcg tgccgccgaa ccgccagggc
      961 gacctctacg cgctgatcgg cgccgtcggt cccggcgtca gcccggccct gatcgcgttg
     1021 ctggtaggcg gcctcggcca ccgcgatcca gcgaccgccg gcgtcgccgc gggcgcgctc
     1081 ggccgcgtcg gcgacgcgtc gagcctcggc ccgctcgccg cggcgctcgc ggcccccgat
     1141 ccgctcggtg aagccgccgc cgccgcgctc gtcgcgttga tcgaccgcca cggacccggc
     1201 acccccggcg tgcaggcgct gctgcgcggc gccggctcgg cgaccggcac cgccgcgtgc
     1261 aacctgtgtc gcgtcgtcgg tgcgctcggc cgcggcggcg acccggtgtt cgtgcagccg
     1321 ctcgtcaccc tgctcggcgc ccccgatccg ctggtgcgcg tcgcggcggc ccgcgctctc
     1381 ggccagatcg gcggcgagca cgagggcgtc acggcgctga cgatggccct cggcgacacc
     1441 gaccccggcg tgcgcgcctc ggcgtgtcgc agcctcggca agctcggtgc cggcgaggcc
     1501 tgccaggccc tgctcgccgt caccaccgac gacagtgccc aggtccgggc cgccgccgtg
     1561 caggcgctcg tcagcctcga caacccggtc ggcctcgccc gcctgcggga gatcgtgctc
     1621 tccgacaacg ccgcgatcgt cgtcatcccg gcgatcgccg gcctcggccg cagcggcggc
     1681 gacctcgacc tcaccctgct gatgagcctg tgcggctcgt ccgaccacga ggtcgccaag
     1741 gcagccgcgc gcggcctggt ccgcttcccg gcccaccgcg ccaccgcggc cgtgctcggc
     1801 ctgctcgccc acgaccgctg ggacgtgcgc tgggccgccg ccgaggtgct cgccgagcgc
     1861 ggcgacctga ccgcgctcga gcccctgcgc cgggtccagc gccaggagac cgacccgctc
     1921 gtgcgccagg tggtcgccag cgcggtcgcc cgcctcgagg gcctggccgc cgcgagcggc
     1981 tcggagagcc ctccgtgagc cggccggacc ccagctcgat cggcggcgta cgcgtgcgga
     2041 tctccgccga ggagacgcgg ctgctgcagg agctgatcct cgagcactgc ggcgtcagcc
     2101 ttggcgccga cgcgggcttc ctgttcgagc gccgcctcgg cccgcgcctc gacacgctcg
     2161 agctcggcag cttcctcgag tatgtgcatc acctcagcta cgacctcgac ggccccgccg
     2221 agctcgagga gtgcgtcgag cgcatcacga cgcacgagac ctacttcttt cgcgagcagt
     2281 tccagctcga cgccttcacc cgggagatcc tcccgcagct gcgcgcgggc ggcccgcgcc
     2341 gcgacgggac cggccccggc ccgtcggtgt ggagcgccgg ctgctcgacg ggcgaggagg
     2401 cgtacacgat cgccatgttg ctgaccgagg cgggtggcat caccgccgcg gccgccggcc
     2461 aggtcgtcgg cagcgacatc agccgcaagg tcctggcgac cgcgcagcag ggcgtttacg
     2521 gcgcggccag ctttcgcacc accgcggacg agctccgcga ccgccgcttc gagccggcgg
     2581 gtgcggggct gtggcgcgtc gcgcccgatg tgcgtcgcat gtgcagcttc gtccacctca
     2641 acctgatgga cgtctcgcgc ttcgccgcgc tcggccgcca cgacgtgatt ttctgccgca
     2701 acgtcctgat gtatttatcg agccaggcgc ggcaccgcat cgtcgagggc ttctacgatg
     2761 cgctggtccc cgggggcttc ctgctgctcg ggcactccga gagcctcctc aacgtcacga
     2821 cccgcttcga cctcgtgcag ctcagcggcg atctggtcta ccgtcgtccg acttgagcac
     2881 cgtccccgcc ggctctgtcc ataccgtcat ggaaaccccc gatccaccgc aacgcgctgc
     2941 gggccagaag gtccgggtcc tcgtcgtcga cgactcggcc tacaaccggc agacgatcag
     3001 cgcgatgctc gagtcggacc cgatggtcga ggtcatcggc cgcgcgtcca acggcaagga
     3061 ggcgctgcgc caggccttcg acttgctccc cgacgtcatc accctcgacc tcgagatgcc
     3121 ggagatggac ggcttcgcct tcctgcgcct gctgatggcc aagcgcccgc tgccggtgat
     3181 catcatctcg agccacagcg agcgcgacaa cgtgttccgc gcgctcgagc tcggcgcgat
     3241 ggacttcatc agcaagcccg gccgccacat cggccccgac ctgcgcgcga tccaggagga
     3301 cctcctggcg aagatcggca tggtccgccg cctcagcgtc gtgcgcctgg tggcccgcgc
     3361 ccaggcgctc gtcgagcagc gcctcgagcg gaccaccggc gagctgccgg cctacgagag
     3421 cggccgccac gacgccaacc tggccgccag caaccagatg gtcggcgtgc tcggcatcgc
     3481 cgcctcgacc ggcggacccc cggcgatcca gcaactgctg tgcgccctgc cgccgacgct
     3541 gccgctggcg atcctcgtcg cccagcacat gccggcccgc ttcacccgcg ccttcgcggc
     3601 ccgcctcgac aagatctgcg cgttccaggt cgtcgaggcc agcgacgcgc aggtgctgcg
     3661 cgcaggggtc gtttacatcg cccccggcgc cgccaacctc gaggtcgaga tcgccggacc
     3721 gggaggcgct cgcgtgcgcg tccgaccgcc gcgcgccggg gcgagcatcg tcccgtctgg
     3781 cgacctcctg ttcaagaccg ccggcgactc gttcggcccg cgcttctgcg ccctgatcct
     3841 gaccggcatg ggatccgacg gccgcgaggg cgccgagctg gcccgccgcg tcggcgccac
     3901 cgtgctcgcc gaggacccgc gtaccgcgat catgccgggc atgcccggct ccgcgatcga
     3961 cgccggggtc gtcgaccagg tgctcgccct cgaggagctg ccggtggcga tccagcgctt
     4021 cgccgatcgt tgccgcg
//
LOCUS       DASWNJ010001964         2265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_600241, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001964 DASWNJ010000000
VERSION     DASWNJ010001964.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600241"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VGB85_32720"
     CDS             complement(<1..711)
                     /locus_tag="VGB85_32720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138911.1"
                     /translation="MQVDHTTDFATTGLGTDLPPEPTDRKQSFPCEQCGSALEFKPGS
                     DTLHCKHCGHTQALPGAAGPGAAVRRHDFREGIARARRQPASTIVKDGHTVQCEGCGA
                     QTVVAGQASRCAFCGSPVVLATAELGEIFTPESLLPFKLDARAAKASYETWVGALWFA
                     PNDLKKLAAQHGMDGVYLPYWAYDSHTTTRYTGERGEHYYETESYTDSEGKRQTRQVR
                     KTRWYPARGTVERQFNDVL"
     gene            complement(730..2022)
                     /locus_tag="VGB85_32725"
     CDS             complement(730..2022)
                     /locus_tag="VGB85_32725"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEY0138912.1"
                     /translation="MKHAQDEMVWIDHNGVAHPLGEVAGARMRKREGAYRMLPTPGHL
                     VFLRYTGNDGRRDLEDGPIVRMAGEITGSGAMTDVVALLGQWAPRGELVMMDGETTRS
                     VFFEHGNIVGVSTDAEDERIGMLLYRYGVLTEDQILPVLERARRGDRFGMAAVELGLV
                     TPEQLYKYLGVQVEEVVSAVLLATDGAYCFLEGFDEARLASRQVTSATALLMQHVTRI
                     DEIRHFRPWIPSADYVPLRLTARSAESSAVWAAIDGLSSVGDIGRSTGLGEFEVTKQL
                     FTLVQAQRVAIQPPRLRGGPLAIVELANTALRSVHQAVDAAGRGTALRNNLAAFARGD
                     YEALLREAGPFEHGDFRAPIVVRNGAALRSTHEVENFIREMLYDYVAFALFSANATLG
                     PTCGLAREIEPLLAKLCPMGQSGRYASTGPRRSSAPAP"
BASE COUNT          297 a          845 c          775 g          348 t
ORIGIN      
        1 gaggacgtcg ttgaactggc gctcgaccgt cccccgcgcc gggtaccagc gggtctttcg
       61 cacctgtcgc gtctgccgct tgccctcgct gtcggtgtag ctctcggtct cgtagtagtg
      121 ctcgccgcgc tcgccggtgt agcgcgtcgt cgtgtgcgag tcgtaggccc agtacggcag
      181 gtacacgccg tccatgccgt gctgcgcggc gagcttcttg aggtcgttgg gcgcgaacca
      241 cagcgcgccg acccacgtct cgtagctcgc cttggccgcg cgcgcgtcga gcttgaacgg
      301 cagcagactc tcgggcgtga agatctcgcc gagctcggcg gtcgccagca ccaccggcga
      361 gccgcagaac gcacagcgcg acgcctgccc ggcgaccacc gtctgcgcgc cgcagccctc
      421 gcactggacc gtgtgcccgt ccttgacgat cgtcgaggcc ggctgccggc gcgcccgggc
      481 gatgccctcg cgaaagtcgt gccgccgcac cgccgcgccc ggccccgccg cgcccgggag
      541 cgcctgcgtg tgcccgcagt gcttgcaatg tagcgtgtcg ctgccgggct tgaactcgag
      601 cgccgagccg cactgctcgc acggaaagct ctgcttgcgg tcggtcggct cggggggcag
      661 gtccgtgccg agccccgtgg tcgcaaagtc cgtggtgtgg tcgacctgca tcgcggcgcc
      721 cagcatacgt cagggcgccg gcgcggacga gcgtcgcgga ccggtcgagg cgtagcggcc
      781 cgactgcccc atcgggcaca gcttggccaa tagaggctcg atctcgcggg cgagcccgca
      841 ggtcggcccg agcgtggcgt tcgcgctgaa cagggcgaac gcgacgtagt cgtagagcat
      901 ctcgcggatg aagttctcga cctcgtgggt cgagcgtaac gccgctccgt tgcgcacgac
      961 gatcggcgcg cgaaagtccc cgtgctcgaa cggcccggcc tcgcgcagca gcgcctcgta
     1021 gtcgccgcgg gcgaaggccg cgaggttgtt gcgcagggcc gtgccgcgcc cggcggcgtc
     1081 gaccgcctgg tgcaccgatc gcagggccgt gttggcgagc tcgacgatcg ccagcggacc
     1141 gccgcgcagc cgcggcggct ggatcgcgac ccgctgggcc tggacgagcg tgaacagctg
     1201 cttggtgacc tcgaactcgc cgagcccggt gctgcgcccg atgtcgccga cgctcgagag
     1261 cccgtcgatc gccgcccaca ccgcgctgct ctcggccgac cgcgcggtca ggcgcagcgg
     1321 cacgtagtcc gccgacggga tccaggggcg aaaatgccgg atctcgtcga tgcgggtcac
     1381 gtgctgcatc agcagcgccg tcgcgctcgt cacctgccgc gaggccagcc gcgcctcgtc
     1441 gaagccctcg aggaagcagt aggcgccgtc ggtcgccagc agcaccgccg acacgacctc
     1501 ctcgacctgg accccgaggt acttgtacag ctgctccggc gtcaccagcc cgagctcgac
     1561 cgccgccatg ccgaagcggt cgcctctgcg cgcccgctcg agcaccggca ggatctggtc
     1621 ctccgtcaac acgccgtagc ggtacagcag catgccgatc cgctcgtcct cggcgtcggt
     1681 cgacacgccg acgatgttgc cgtgctcgaa gaacacgctg cgcgtcgtct cgccatccat
     1741 catcaccagc tcgccgcgcg gcgcccattg cccgagcagc gcgacgacgt ccgtcatcgc
     1801 ccccgacccc gtgatctcgc cggccatccg cacgatcggc ccgtcctcga ggtcgcgccg
     1861 cccgtcgttg ccggtgtacc gcaggaacac caggtgcccg ggcgtcggca acatgcgata
     1921 cgccccctcc cgcttgcgca tccgggcccc ggccacctcc ccgagcgggt gcgccacgcc
     1981 attgtggtca atccagacca tctcgtcctg ggcgtgcttc atgttcgctc gcccagcata
     2041 tcagctctcc gggggccgcg gcccacgccg ggccccttcc gcgcgctgga gacaggtctg
     2101 tgcgggcggc ccgaggcgcc gatcacggca ccgggtgtcg cgttcgttgg gggacgcgca
     2161 acgcactcgc cggcttgcga cgagactccc gggctggtct ttattgcggg cttgccggat
     2221 cctcgggtga tgacgcgatg catggtgctc gtcgggctgg cgttc
//
LOCUS       DASWNJ010001965         1849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_417352, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001965 DASWNJ010000000
VERSION     DASWNJ010001965.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417352"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VGB85_32730"
     CDS             complement(<1..353)
                     /locus_tag="VGB85_32730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY0138913.1"
                     /translation="MHPARPRRRPRGLSRLELLLVLAILGILAVLAVGELRTLSRRSK
                     TEEAVRGLADMFNGVAAHFNEQRPDYGAPGLPPHRCPHTPDQPFLGEQAPTPSLALRC
                     ADGPGGRCTPRQGADG"
     gene            complement(422..1153)
                     /locus_tag="VGB85_32735"
     CDS             complement(422..1153)
                     /locus_tag="VGB85_32735"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0138914.1"
                     /translation="MRAEKPPDRLSGLAQALAGEALLQHVAGRRVLDIGSGSPEIAHW
                     VRPIAASLDTVDLAPCTDESGEIRLPQADARYDVVYSLRTLAHLGHDEASSDLGVRSL
                     LAEAARVTVPGGVILVDINNPRSLRGFFYGIRRPITVVATGSVVLADAQHRVTRHDTL
                     ARLLRVAPPSLEAVAVHGIRVLVPIARALRIPILGRLLAASEWWARDSFLRGFGAHLL
                     VALRKDEDADRKRLPDVEMKPTPTY"
     gene            1354..>1849
                     /locus_tag="VGB85_32740"
     CDS             1354..>1849
                     /locus_tag="VGB85_32740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0138915.1"
                     /translation="MPTKILAIDDSKTMRLAIKITFAAEDCEVVAVSKGSEAVARAKQ
                     MPADILIVDAVLAAGEPSGYDVARQLRADPETAHIPVLILVSNQTGIDEGQVQASGAN
                     GAMTKPFDTQELMERVNALVSGKSVASKPAPVRQPVSTTVGSTTSPTPATAARPAPAA
                     AAAAP"
BASE COUNT          266 a          625 c          723 g          235 t
ORIGIN      
        1 ccatcggcgc cctgacgcgg ggtacagcgg ccgccggggc cgtcggcgca gcgcagcgcg
       61 agggacgggg tgggggcctg ttcaccgagg aacggctggt cgggcgtgtg cgggcagcgg
      121 tgcggcggca ggccgggcgc gccgtagtcg gggcgttgct cgttgaagtg agcggcgacg
      181 ccgttgaaca tgtcggcgag gccacgcacc gcctcctcgg tcttgctgcg gcgtgacagc
      241 gtgcgcagct caccgaccgc gagcaccgcg aggatgccga ggatcgccag cacgagcagc
      301 agctcgagcc gggacaggcc ccgggggcgt cggcgtgggc gggcgggatg catgcggaga
      361 tctaggtaaa tggatctcga aggtcgatca ctgcgggccg acgcgcaagg atcctgcctg
      421 atcagtaagt cggtgtaggt ttcatctcta catcgggcaa acgcttgcgg tcggcgtcct
      481 cgtccttgcg cagggcgacg agcaggtggg ctccgaagcc gcgcaggaag ctgtcgcggg
      541 cccaccactc ggaggcggcc aggaggcggc cgaggatcgg gatccgcagc gcgcgcgcga
      601 tcgggacgag cacgcggatc ccgtgcacgg cgaccgcctc gaggctcggc ggggcgacgc
      661 gcagcaggcg ggcgagggtg tcgtggcggg tgacgcggtg ctgggcgtcg gccagcacca
      721 cgctgccggt ggcgacgacg gtgatcgggc ggcggatgcc gtagaagaac ccgcgcaggc
      781 tgcgggggtt gttgatgtcg acgaggatga cgccgccggg cacggtcacg cgcgcggcct
      841 cggccagcag gctgcgcacg ccgaggtccg acgaggcctc gtcgtggccg aggtgcgcga
      901 gcgtccgcag gctgtagacc acgtcgtagc gggcgtcggc ctggggcagc cggatctcgc
      961 cggattcatc ggtgcagggg gcgaggtcga cggtgtcgag cgaggcggcg atcgggcgca
     1021 cccagtgggc gatctccggc gagcctgaac cgatgtcgag gacacggcgg ccggcgacgt
     1081 gctgcagcag ggcctcgccg gcgagcgcct gggcgagccc ggagaggcgg tccgggggct
     1141 tttcggcccg cacgggcgcg ccccaccagg tcgcgctggc ggccggaccc cgcctcccgg
     1201 ccgccaggga ggcgccgggg agcgaatctc cggggtctcc cggggcggtc ggacgcgcag
     1261 tgttcccggg cgggagcccc cggcccgtgg gcattggttt cgttgaccgg ctcacgtgct
     1321 ccatggtacc cctgctgcct cgaaggagcc ctgatgccga ccaagatcct agcgatcgat
     1381 gacagcaaga cgatgcgcct cgccatcaag atcaccttcg cggcggaaga ctgcgaagtg
     1441 gtcgcggtga gcaagggctc ggaggcggtc gcgcgcgcca agcagatgcc ggccgacatc
     1501 ctcatcgtcg acgccgtgct cgccgccggt gagccctccg gttacgacgt cgctcgccag
     1561 ctccgcgccg accccgagac cgcccacatt ccggtgctga tcctcgtgtc gaaccagacc
     1621 ggcatcgacg agggccaggt ccaggcgtcc ggcgcgaacg gggcgatgac caagcccttc
     1681 gacacgcaag agctgatgga gcgggtcaac gcgctggtct ccggcaagag cgtcgccagc
     1741 aagcccgcgc ctgtccgcca gccggtctcg accacggtcg gctcgaccac ctcgccgacg
     1801 cccgcgaccg cggcccgtcc ggccccggcc gccgccgctg cggcgcccg
//
LOCUS       DASWNJ010001966         1883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_139754, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001966 DASWNJ010000000
VERSION     DASWNJ010001966.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139754"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VGB85_32745"
     CDS             complement(<1..564)
                     /locus_tag="VGB85_32745"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY0138916.1"
                     /translation="MTVYGVEALRRVPGGIASRDIAGGAGDGSDADAALASGSEDTGV
                     SGIVPRMSDTPEEVPAGVHEHRRAVVPEDIDELGHVNNLRYLEWMMAAAIEHSEAVGW
                     DGARYAALGNAWVVRSHAIEYLRPAFAGDAVVVRTWVSEMAKVSSRRKYAIAREGGPV
                     LARAETLWVYINRRTHTLDRVPADLRAA"
     gene            656..1675
                     /locus_tag="VGB85_32750"
     CDS             656..1675
                     /locus_tag="VGB85_32750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138917.1"
                     /translation="MLPPRYAQWLTELLGGSLPEEHAATCSRCAMQVDSVEEGYRFRA
                     DAKCCTYVPALPNFLVGAALRELPPGPARAGLDERVADPGVCTPHGLDVSDDDRKRYQ
                     AIVAAETFGRDPDLRCPHYIDQQGGLCGIWRHRNGVCATWFCKHDRGAAGQRFWQAVE
                     VLLAHVERELSHWCACQLLYHGAASEPASPDEGLGAWDAWSGRPGEYYEHTAALVDEL
                     AWPEIAVIAGPDLGPLVDRVKATYAELQPRPTDPSLVPLRRPSRNLVAADLRVSHRGP
                     ESARIVTYSDSDPIAMPSALLEILPRFDGRPTDEVLAELASDGVVLTDDLVAQLVDFA
                     VLADP"
BASE COUNT          237 a          778 c          622 g          246 t
ORIGIN      
        1 cgcggcgcgc agatccgccg gcacgcggtc gagggtgtgg gtccggcgat tgatgtagac
       61 ccacagggtc tcggcccgcg ccagcaccgg gccgccctca cgggcgatcg catacttgcg
      121 ccgcgagctg accttggcca tctcgctgac ccaggtgcgc acgaccaccg cgtcgccggc
      181 gaaggccggg cgcaggtact cgatcgcgtg cgatcgcacg acccaggcgt tgccgagcgc
      241 ggcataacga gcgccgtccc agccgaccgc ctcggagtgc tcgatcgcgg ccgccatcat
      301 ccactcgagg tagcgcaggt tgttgacgtg cccgagctcg tcgatgtcct ccggcacgac
      361 ggctcggcgg tgctcgtgga cccccgccgg gacctcctcg ggcgtgtcgc tcatgcgcgg
      421 cacgataccc gaaaccccgg tgtcctcgct cccgctcgcc agcgccgcgt ccgcgtcact
      481 tccgtcgccg gcgccgcctg cgatgtcgcg cgacgcgatg ccgcccggca cccgccgcag
      541 cgcctcgacc ccgtagaccg tcacgccccg ccgagcccgc accggatccc cgggacgatg
      601 gtacgctggg gtccggcacc gggacaagcg acccggtcgc cgtcccacgc tcctcatgct
      661 gccgccgcgc tacgctcaat ggctgacgga gctgctcggc gggagcttgc ccgaggagca
      721 cgccgcgacc tgctcgcgct gcgccatgca ggtcgactcg gtcgaggagg gctaccgctt
      781 ccgcgccgac gcgaagtgct gcacctacgt gcccgcgctg cccaacttcc tcgtcggcgc
      841 cgcgctgcgc gagctgccgc caggcccggc gcgcgcgggc ctcgacgagc gtgtcgccga
      901 ccccggcgtg tgcaccccgc acggcctcga cgtcagcgac gacgaccgca agcgttacca
      961 ggcgatcgtc gccgccgaga ccttcgggcg cgaccccgat ctgcgctgcc cgcactacat
     1021 cgatcagcag ggcggcctgt gcgggatctg gcgccaccgc aacggcgtgt gcgcgacctg
     1081 gttctgcaag cacgaccgcg gcgccgccgg gcagcggttc tggcaggccg tcgaggtgct
     1141 gctcgcccac gtcgagcgcg agctgagcca ctggtgcgcc tgccagctgc tgtaccacgg
     1201 cgccgcctcc gagcccgcca gccccgacga gggcctcggt gcgtgggacg cctggagcgg
     1261 ccgccccggc gagtactacg agcacaccgc cgccctcgtc gacgagctcg cctggcccga
     1321 gatcgccgtg atcgccggcc ccgacctcgg cccgctcgtc gatcgcgtca aggcgaccta
     1381 cgccgagctg cagccgcgcc ccacggaccc ctcgctggtg cccctgcgcc gtccatcacg
     1441 caatttggtc gccgccgacc tgcgcgtcag ccaccgcggc cccgagagcg cccgcatcgt
     1501 cacttacagc gacagcgacc cgatcgccat gcccagcgcg ctgctcgaga tcctcccgcg
     1561 cttcgacggc cgcccgaccg acgaggtgct cgccgagctg gccagcgacg gcgtcgtgct
     1621 gaccgacgac ctcgtcgcgc agctcgtcga cttcgccgtg ctcgccgatc cgtgagcgtg
     1681 catcaccgcg cgggatcggg tagcttggcc cggcgatgct ccgcgccgat gccctcgccc
     1741 tgatcgcctg ctgcggcctc gccgtggcct gcggcgacgc caccgagcag cccgccccgg
     1801 ccgccgacga ggcccccgcg gccccggccg aacccgccgc gccggcgatc cccgtcccgc
     1861 cgccgctcga cgacgccttc cac
//
LOCUS       DASWNJ010001967         3071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_457483, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001967 DASWNJ010000000
VERSION     DASWNJ010001967.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457483"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1594
                     /locus_tag="VGB85_32755"
     CDS             <1..1594
                     /locus_tag="VGB85_32755"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0138918.1"
                     /translation="TPSNLPAIVASVGDLEFGDVDDDGDLDVILADWGADTPVDDMNN
                     SSGGVTQLWLQDEKPGFFGEGSAVFVDATLSQMPNVGIRWSWDLEFVDLDNDYDLDAV
                     VSCFACTKASLFLFANDGKGTFTQVPMTNAPQGAGALSVEAINLDGDAFLDLVTTHDA
                     TGGRNRVLLADGQGAFADWSGQLWPMLENPSSFDTMAAFLDFNSDARPDIVLGALQAG
                     LNKYPDRLLENQNGKFKANTFAFAETKASSGTYAIVLADFNLDRRLDVAMSQNENAFE
                     KKVFLGSEVELQPDTEAPQFVNYQKLPADIEFGQDYMLRLRCHDNKSPLMLHDFQRAA
                     DGNQDGRPYVESWNSTPADPNTTPPDNISAPGQWYGEYLWRVGFSVPLEADPFVYRIC
                     AIDAAGNKRCTEPESVDNPAQETGTETESTTTTGDAATLPDTITDPTTETTQGTVGEA
                     ESLTGEANTDPNVPTTGAGPLTDTVASASESENESIETLDADELDDDGCGCDTTQSPT
                     RGVLSSLALLGLLGLRRRRARA"
     gene            complement(1769..>3071)
                     /locus_tag="VGB85_32760"
     CDS             complement(1769..>3071)
                     /locus_tag="VGB85_32760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HEY0138919.1"
                     /translation="YAGETLPPRRIGLYDGLLALSEKGKPTTTVLLDQIVVNSPPQGD
                     LTWYVLEGDKGVNPKEFVQAKALPGGGQAKLFDVFNPVNDPFNRSINTTVPPQMDVVG
                     VDIDKFSLAQVLEQDDTSLEITYSAGLDKYWIAFNLVGVDVFQALFNVESNITWQLTG
                     DVGGDGEPSPGDTVTYTIHIENSGTAPGNVMVTDEIPVEAASWILTETGGGMDKTMGD
                     TLVVDGISLLPAQGTDVVFEIVIADVPDQTSIIDTAELDDGDSAIELTAPELIVRVDS
                     DGDAKFDADDNCPEVPNPDQADADGNGVGDVCEGMETNASATESGSPDTDTATATQSA
                     TESATAASITDSGTGGSDSNSGVPTTDGPTSGSASASATATASDGDTESGSDTESGSA
                     GLTSGEIDDEGCGCRSDDSDGPVAALLVLLGLAGVRRRRQG"
BASE COUNT          468 a         1132 c          999 g          472 t
ORIGIN      
        1 gaccccgtcg aacctcccgg cgatcgtcgc cagcgtcggc gacctcgagt tcggcgacgt
       61 cgacgacgac ggcgacctcg acgtcatcct cgccgactgg ggcgccgaca cgccggtcga
      121 cgacatgaac aacagctccg gcggcgtcac ccagctgtgg ctgcaggacg agaagcccgg
      181 cttcttcggc gagggctccg cggtgttcgt cgacgccacc ctctcccaga tgcccaacgt
      241 cggcatccgc tggtcgtggg acctcgagtt cgtcgacctc gacaacgact acgacctcga
      301 cgccgtcgtg tcctgcttcg cgtgtaccaa ggcgagcctg ttcctgttcg ccaacgacgg
      361 caagggcacc ttcacccagg tcccgatgac caacgcgccg cagggcgccg gcgccctcag
      421 cgtcgaggcg atcaacctcg acggcgacgc cttcctcgac ctcgtcacca cgcacgacgc
      481 caccggcggc cgcaaccgcg tgctgctcgc cgacggtcag ggcgccttcg ccgactggag
      541 cggccagctg tggccgatgc tcgagaaccc gtccagcttc gacacgatgg cggccttcct
      601 cgacttcaac tccgacgccc gcccggacat cgtgctcggc gcgctgcagg ccggcctcaa
      661 caagtacccc gaccgcctgc tcgagaacca aaacggcaag ttcaaggcca acaccttcgc
      721 gttcgccgag accaaggcct cgagcggcac ctacgcgatc gtcctcgccg acttcaacct
      781 cgaccgccgc ctcgacgtcg ccatgtcgca gaacgagaac gccttcgaga agaaggtgtt
      841 cctcggcagc gaggtcgagc tccagcccga caccgaggcc ccgcagttcg tcaattatca
      901 aaagctcccg gccgacatcg agttcggcca ggactacatg ctgcgtctgc gctgccacga
      961 caacaagtcg ccgctgatgc tccacgactt ccagcgcgcc gccgacggca accaggacgg
     1021 tcgcccctac gtcgagtcgt ggaactccac gccggccgac ccgaacacca ccccgccgga
     1081 caacatctcc gcccccggcc agtggtacgg cgagtacctg tggcgcgtcg gcttcagcgt
     1141 gcccctcgag gccgacccgt tcgtctatcg catctgtgcg atcgacgccg ccggcaacaa
     1201 gcgctgcacc gagccggaga gcgtcgacaa cccggcccag gagacgggga ccgagacgga
     1261 gtcgacgacc accaccggcg acgccgccac gctgcccgat acgatcaccg acccgaccac
     1321 ggagaccacg cagggcacgg tgggcgaggc ggagagcttg accggcgagg ccaacaccga
     1381 ccccaacgtc ccgacgaccg gcgccgggcc gctgacggac accgtcgcca gcgccagcga
     1441 gagcgagaac gagagcatcg agaccctcga cgccgacgag ctcgacgacg acggctgcgg
     1501 ctgcgacacc acgcagtcgc cgacccgcgg cgtgctgtcc tcgctcgcgc tgctcggcct
     1561 gctcggcctg cgccgccgtc gcgcccgcgc ctgacacacg cgagggtgac cgcgaagcaa
     1621 catcgcaggg gccgcgggtg accgccggcc cctgcggcgt ttaattaatg agcctgaccg
     1681 gagcatgacg gtcgctccga cccgcaagcg cgaagcggcc gcgacgacgc cggagcgtcg
     1741 cgcggccact cgttgctctc tcgccgcttt agccctgacg gcggcggcgc acgccggcga
     1801 ggccgagcag caccagcagc gcggcgaccg ggccgtcgct gtcgtcgctg cggcagccgc
     1861 agccctcgtc gtcgatctcc cccgaggtca gcccggcgct gccgctctcc gtgtcgctcc
     1921 cggactcggt gtcgccgtcg ctcgcggtcg ccgtcgcact cgcgctcgcg ctgcccgagg
     1981 tcgggccgtc ggtcgtcggg acgccgctgt tcgagtcgct gccgccggtg ccgctgtcgg
     2041 tgatcgacgc ggccgtcgcg ctctcggtcg ccgactgtgt cgccgtcgcc gtatccgtgt
     2101 ccggcgaacc gctctcggtc gcgctggcgt tggtctccat gccctcgcag acgtcgccga
     2161 cgccgttgcc gtccgcgtcg gcctggtcgg ggttggggac ctcggggcag ttgtcgtcgg
     2221 cgtcgaactt ggcgtcgccg tcgctgtcga cgcggacgat cagctcgggc gcggtcagct
     2281 cgatcgccga gtcgccgtcg tcgagctcgg cggtgtcgat gatcgaggtc tggtcgggca
     2341 cgtccgcgat cacgatctcg aacacgacgt cggtaccctg cgccggcaac aggctgatgc
     2401 cgtcgaccac gagcgtgtcg cccatcgtct tgtccatgcc gccgccggtc tcggtcagga
     2461 tccacgaggc cgcctcgacg gggatctcgt cggtgaccat gacgttgccg ggcgcggtgc
     2521 ccgagttctc gatgtggatc gtgtaggtca cggtgtcgcc gggggatggc tcgccgtcgc
     2581 cgccgacgtc gccggtcagc tgccaggtga tgttcgactc gacgttgaac agcgcctgga
     2641 acacgtcgac gccgacgagg ttgaacgcga tccaatactt gtcgaggccg gcgctgtagg
     2701 tgatctcgag gctggtgtcg tcttgctcga gcacctgcgc gagcgagaat ttgtcgatgt
     2761 cgacgccgac gacgtccatc tgcggcggca cggtggtgtt gatcgaccgg ttgaacgggt
     2821 cgttgaccgg gttgaagacg tcgaacagct tggcctggcc gccgccgggc agcgccttgg
     2881 cctgcacgaa ctccttcggg ttgaccccct tgtcgccctc cagcacatac caggtcaggt
     2941 caccctgcgg cgggctgttg acgacgatct ggtcgagcag caccgtcgtc gtcggtttgc
     3001 ccttctccga cagggcgagc aggccgtcgt acaggccgat ccgccgcggt ggcagggtct
     3061 cgccggcata t
//
LOCUS       DASWNJ010001968         6798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_600280, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001968 DASWNJ010000000
VERSION     DASWNJ010001968.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6798
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600280"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1599
                     /locus_tag="VGB85_32765"
     CDS             <1..1599
                     /locus_tag="VGB85_32765"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEY0138920.1"
                     /translation="RDDASFTTALAELAATTPAALGLVVLLAAPHPDLPREAAHDPRR
                     LRAELLALAGGDRVFILHLACLHDLRPLQALLAGTDQSVPYVVFHDAHFSLAALQPLG
                     VTPPRFGCTLIAATLLAEGTGVRHDTLELAALVTRHLERGLPGQVQQGQVSPAIARYP
                     VAAAAADSLVPLLRALTPVLRERGLSRVYDLECRLLPAVLAMESAGVGLDVAGFNRIA
                     AGWQRERDTNPAPERIAQLDKLLSTYANWPRDFVADDGRMRPHLHPLATDSGRFACSQ
                     PNLQQVPAEHTAPGLRACFVPAPGNVLIIADYAQIELRVAAQLAPCAALRRVFQEGRD
                     PHRATAATLAGKPESEISSHERKLAKAVNFGFLFGMGARRFQDYALASYGLELDHAAA
                     QAARDAFFATFPGIAAWHRKIGNLEPRSHREDIVVHTALGRRKRFLAGHFSFNAALNI
                     PVQGTAAEGFKRAMIDLHAALGPLGGRGVLCVHDEYLAEVPEDRAEAARVLVSQTMEA
                     AMATVVPGVPIVAEAHIARSWSDK"
     gene            1754..3322
                     /locus_tag="VGB85_32770"
     CDS             1754..3322
                     /locus_tag="VGB85_32770"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEY0138921.1"
                     /translation="MHAFTTRRTFVRGTATGAALLGASGLGLLGGCHRTSAPGTTMIP
                     GDPATSTLSDHLGFFSVDESVLTRVMAELTSRGADAADLYFQHSRDNSITMEDGIISR
                     ASSSVDQGVGLRVVVGDQTGYAFTEDLSLAAMIAAARTAASIAHGSKAVAPVAYTHGK
                     TADFYTLKQPWSDIGVAQKLPRIEQLNALARAADPAIEKVTVSWAGGEEVVLIADMHG
                     RIHTDVRPMTRMWCMVTAKKNGVSQANSANLAGRHGFEWYTDDRLAFVAKEAVNRTMI
                     LFEAKRPPAGELPVVLAAGASGILLHEAIGHGMEADFNRKGTSIYADMIGKKVAPDFV
                     TIVDDATQPGERGALNVDDEGNPTEKTVLVDHGVMASYLHDSISARHYGHKGTGSGRR
                     ESFRHAPLPRMRSTYMENGPHTREEIIASVKKGIIAETFTNGQVQIGAGDFTFYIKNG
                     WLIEDGKITAPIKDINIIGSGPEALRNVTMVGNDTKLDFGGWTCGKDGQSVPVSQGMP
                     TTLVSKMTVGGENA"
     gene            3315..4688
                     /locus_tag="VGB85_32775"
     CDS             3315..4688
                     /locus_tag="VGB85_32775"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEY0138922.1"
                     /translation="MPDTTSRSSGPETSRPSGPESNDLLKSATDAVEIARKAGAQDAW
                     ASLSRDRSVSYTYRDGKIEKVEESTSRGLGISLYVDDRYSSHSTTDLRPDRLAAFIRE
                     AVALTRALQPDPHRKIPDPALFQGRPTVDLERRDPGLADLTPQQREAWCAEMDAKAHS
                     DKRVISAESGLFSGESQGASASSNGFSGTWEGGYIGYNCTATVREGEDKRPEGSASAV
                     GRFLAGLPEPTKIAEKALKRTLDRLGSKKGPTIRATMVVDPEAAISLISRALGPASAA
                     AIQQGRSFWAGKIGQPLLGDKLTVTDDPLIPRGLGSRHFDGEGISARRMPIIERGVVK
                     NLYVDTYYGRKLGMAPTTGSASNRVVALGDRNLQELLAQAGSGIYVTSWLGGNSDSTT
                     GDFSLGLRGHLIENGKIGAPIGEMNVTGNLPTLYKSLVAVGNDPYPYSSLRAPTLVFE
                     NVQFSGA"
     gene            complement(4796..5302)
                     /locus_tag="VGB85_32780"
     CDS             complement(4796..5302)
                     /locus_tag="VGB85_32780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138923.1"
                     /translation="MKLLLALPLALTLASCGGDTKTGDAKTGDAKTGDAKSSGGSTLE
                     LPKVGLKADAPAGTEVGDVIGGGVGHMVQGPGLVVNVETASETAPKTVDDAKKESEMF
                     DGVKNLKDEKLPDGWLITFENVGGMGANYHVNTRREIDGKAITCNTMVSTPEQQNNAV
                     NFCKSLKK"
     gene            complement(5892..6251)
                     /locus_tag="VGB85_32785"
     CDS             complement(5892..6251)
                     /locus_tag="VGB85_32785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138924.1"
                     /translation="MARRARGATWWLLAAVVGVSLGLACKARSRGFCGNYAEAVSECC
                     SDHEYAHLRAICDQDLGIAWSVGAGCAQSVRDLYDCAEERACVHECEEESPDPCAQQR
                     QAMLGICSESPSPPPPP"
     gene            6376..>6798
                     /locus_tag="VGB85_32790"
     CDS             6376..>6798
                     /locus_tag="VGB85_32790"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY0138925.1"
                     /translation="MHELLDASAVTLARWIRQRKLSPVELLEALIDRIADVNPAINAV
                     IAERYTLALAEARAAEHRAMHEPADTLPPLLGLPYTAKEYIRATGMPLSAGIWSRRHV
                     RSDRDAETVARLGRAGAILVGITNVPEGGLWMETYHDVY"
BASE COUNT         1071 a         2665 c         2092 g          970 t
ORIGIN      
        1 cgcgacgacg cctcgttcac gaccgcgctc gccgagctcg ccgccaccac ccccgccgcg
       61 ctcggcctcg tcgtattgct cgcagcgccc caccccgacc tgcctcgtga ggccgcccac
      121 gacccccgcc gcctgcgcgc cgagctgctc gccctcgccg gcggggacag ggtctttatc
      181 ctccacctcg cctgcctgca cgacctgcgg ccgctgcaag cgctgctcgc cggcaccgac
      241 cagagcgtcc cctacgtcgt cttccacgac gcccacttca gcctcgcggc cctgcagccg
      301 ctgggcgtca ctccgccgcg ctttggctgc accctgatcg ccgcgacctt gctcgccgag
      361 ggcaccggcg tccgccacga caccctggag ctcgccgcgc tggtgacccg acacctggag
      421 cgcggcctgc ccggtcaggt ccagcagggc caggtcagcc cggcgatcgc ccgctacccg
      481 gtcgccgccg ccgccgccga ctccctggtc ccgttgttgc gcgcgctgac ccccgtgctg
      541 cgcgagcgcg gcctgtcccg cgtctacgac ctcgagtgcc ggctgctgcc cgccgtgctc
      601 gccatggagt ccgccggcgt cggcctcgac gtcgccggtt ttaataggat cgccgcgggc
      661 tggcagcgcg agcgcgacac caaccccgcc cccgagcgga tcgcccagct cgacaaattg
      721 ctctcgacct acgccaattg gccgcgggac ttcgtcgccg acgacggccg catgcgcccg
      781 cacctgcacc ccctggcgac cgactccggg cggtttgcct gctcgcagcc gaacctgcaa
      841 caggtccccg ccgagcatac cgcgccgggc ctgcgcgcct gcttcgtgcc ggcccccggc
      901 aacgtcctga tcatcgccga ctacgcgcag atcgagctgc gagtcgccgc ccagctggcc
      961 ccctgcgcgg ccctgcggcg cgtgtttcag gagggtaggg acccccaccg cgcgaccgcg
     1021 gcgaccctcg ccggcaagcc cgagtccgag atctccagcc acgagcgcaa gctcgccaag
     1081 gcggtcaact tcgggttttt gttcggcatg ggcgcgcgcc gcttccagga ctacgcgctc
     1141 gccagctacg gcctcgagct cgaccacgcc gccgcccagg ccgcgcgcga cgccttcttc
     1201 gccaccttcc cgggcatcgc cgcctggcac cgaaagatcg gcaacctcga gccgcgcagc
     1261 catcgtgagg acatcgtcgt ccacaccgcg ctcggccggc gcaagcgctt cctcgccggg
     1321 cacttcagct tcaacgccgc cctcaacatc cccgtccagg ggaccgccgc cgagggcttc
     1381 aagcgcgcga tgatcgacct ccacgctgcc ctcgggccgc tcggcggccg gggcgtgttg
     1441 tgtgtgcacg acgagtacct cgccgaggtg cccgaggacc gggccgaggc cgcccgcgtc
     1501 ctggtctcgc agaccatgga ggcggccatg gcgaccgtcg ttccgggcgt cccgatcgtc
     1561 gccgaggccc acatcgcccg cagctggtcc gacaagtgag gccacgcgga cccgtcgccc
     1621 ggccccgccg cgcccggtat ctccgcattc ccccgatcct gctaccgctg tgaaacctca
     1681 cgggctcggg cgcgctccaa gtctccacga cggcacggcg ccgtcggatc ctcgtcccgg
     1741 gagactacaa ccgatgcatg ctttcacgac ccgtcgcacc ttcgtgcgcg gaacggccac
     1801 cggcgctgcc ctgctcggcg cctccggcct cggcctcctc ggtgggtgcc atcgcacctc
     1861 cgcccccgga accacgatga tcccaggcga ccccgctacc tccactctct cggaccacct
     1921 cggcttcttc tcggtcgacg agtctgtcct cacccgcgtg atggccgagc tcacctcccg
     1981 cggcgccgac gcggccgacc tctatttcca gcacagccgc gacaacagca tcaccatgga
     2041 ggacggcatc atcagccgcg cgtcctcctc ggtcgatcag ggcgtcggcc tgcgcgtcgt
     2101 cgtcggcgac cagaccggct acgccttcac cgaggacctc tccctcgccg cgatgatcgc
     2161 cgccgcgcgt actgcggcca gcatcgccca cggcagcaag gcggtcgccc cggtcgccta
     2221 cacccacggc aagaccgccg acttttacac gctcaagcag ccgtggtcgg acatcggcgt
     2281 cgcgcaaaag ctcccgcgca tcgagcagct caatgccctg gcccgcgccg ccgacccggc
     2341 gatcgaaaaa gtcacggtgt cctgggccgg cggcgaggag gtcgtgctga tcgccgacat
     2401 gcacggccgg atccacaccg acgtgcgccc gatgacccgc atgtggtgca tggtgaccgc
     2461 caagaagaac ggggtcagcc aggccaactc ggccaacctc gccggtcgtc acggcttcga
     2521 gtggtacacc gatgaccgcc tcgccttcgt cgccaaggag gccgtcaacc gcacgatgat
     2581 cctgttcgag gccaagcgcc cgcccgcggg cgagctgccg gtcgtgctcg ccgcgggtgc
     2641 ctccggtatc ttgctgcacg aggcgatcgg tcacggcatg gaggccgact tcaaccgcaa
     2701 gggcaccagc atctacgccg acatgatcgg caagaaggtc gcccccgact tcgtcaccat
     2761 cgtcgacgac gccacccagc ccggcgagcg cggcgcgctc aacgtcgacg acgagggcaa
     2821 cccgaccgag aagaccgtgc tcgtcgacca cggcgtgatg gcctcgtacc tgcacgacag
     2881 catcagcgcg cgccactacg gccacaaggg caccggctcg ggccggcgcg agtcgttccg
     2941 ccacgccccg ctgccgcgca tgcgcagcac gtacatggag aacggcccgc acacgcgcga
     3001 ggagatcatc gcctccgtca agaagggcat catcgccgag acctttacca acggtcaggt
     3061 ccagatcggc gccggcgact tcaccttcta tatcaagaac ggctggctga tcgaggacgg
     3121 caagatcacc gccccgatca aggacatcaa catcatcggc tcgggccccg aggccctgcg
     3181 caacgtgacg atggtcggca acgacaccaa gctcgacttc ggcggctgga cctgcggcaa
     3241 ggacggccag tcggtcccgg tctcgcaggg catgccgacc accctcgtct cgaagatgac
     3301 cgtcggaggc gaaaatgcct gataccactt cacggtcctc cggacctgag acatcacggc
     3361 cctccgggcc cgagtcgaac gacctcctca agagtgcgac cgacgccgtc gagatcgccc
     3421 gcaaggccgg agcccaggac gcctgggcct cgctctcgcg cgaccgctcc gtcagctaca
     3481 cctaccgcga cggcaagatc gagaaggtcg aggagagcac ctcgcgcggc ctcggcatca
     3541 gcctctacgt cgacgaccgc tactcgagcc acagcaccac cgacctgcgg cccgatcgcc
     3601 tcgccgcgtt catccgcgag gccgtcgccc tgacgcgcgc gctccagccc gacccgcacc
     3661 gaaagatccc cgaccccgcg ctgttccagg gccggccgac cgtcgacctc gagcgcaggg
     3721 accccggcct cgccgacctc accccgcagc agcgcgaggc ctggtgcgcc gagatggacg
     3781 ccaaggccca cagcgacaag cgcgtgatct cggccgagag cggcctgttc agcggcgagt
     3841 cgcagggcgc ctcggccagc tccaacggct tctcgggcac ctgggagggc ggctacatcg
     3901 gctacaactg caccgcgacc gtgcgcgagg gcgaggacaa gcgccccgag ggttcggcct
     3961 ccgccgtcgg ccgcttcctc gccggtctgc ccgagccgac caagatcgcc gagaaggcgc
     4021 tcaagcggac cctcgatcgc ctcggcagca aaaagggccc gacgatccgc gccacgatgg
     4081 tcgtcgaccc cgaggccgcg atctcgctga tctcccgcgc gctcggcccg gccagcgccg
     4141 ccgcgatcca gcagggtcgc tcgttctggg ccggcaagat cggccagccg ctgctcggcg
     4201 acaagctgac cgtcaccgac gacccgctga tcccccgcgg cctcggctcc cggcacttcg
     4261 acggcgaggg catctccgcc aggcgcatgc cgatcatcga gcgcggcgtc gtcaagaacc
     4321 tctacgtcga cacctactac ggccgcaagc tcggcatggc gccgaccacc ggctcggcct
     4381 cgaaccgcgt cgtcgcgctc ggcgaccgga acctgcagga gctgctcgcg caggccggca
     4441 gcggcatcta cgtgacctcc tggctcggcg gcaacagcga cagcaccacc ggcgacttct
     4501 cgctcggcct gcgcggtcac ctgatcgaaa acggcaagat cggcgcgccg atcggcgaga
     4561 tgaacgtcac cggcaacctg ccgacgcttt acaagagcct cgtcgccgtc ggcaacgacc
     4621 cgtacccgta ctcgagcctg cgcgccccga ccctcgtgtt cgagaacgtg cagttcagcg
     4681 gcgcctgacc gcctgtccct gcggacaggt cacctgaaag cgagtgaaat gaaaacgggc
     4741 ctggcgcgag ccagacccgt cttttacgtg cccacgccgg ccgcagccgg gcggctcact
     4801 tcttgaggct cttgcagaag ttgaccgcgt tgttctgctg ctcgggcgtc gacaccatcg
     4861 tgttgcaggt gatcgccttg ccgtcgatct cgcggcgggt gttgacgtgg tagttggcgc
     4921 ccatgccacc gacgttctcg aaggtgatca gccagccgtc ggggagcttc tcgtccttga
     4981 ggttcttgac cccgtcgaac atctcggact ccttcttggc gtcgtcgacc gtcttgggcg
     5041 ccgtctccga cgccgtctcg acgttgacga ccaggcccgg gccctggacc atgtgaccca
     5101 cgccgccacc gatcacgtcg ccgacctcgg tgccggctgg cgcgtcggcc ttgaggccga
     5161 ccttgggcag ctcgagggtg gagccgccgg aggacttggc gtcgccggtc ttggcgtcgc
     5221 cggtcttggc gtcgccggtc ttggtatcgc cgccacagga ggccagggtc agcgcgaggg
     5281 ggagagcgag caggagcttc atgatgtcct tcgagttgcg agatcgcgtg cgatcgggat
     5341 gcgggagcga gggcgcccac gcggcccgag gatccacgcc acgcgcgccg ccgtcaaggg
     5401 cgtatgcgcg caagaacgcc aaactcccga gcccccgtcg tcggggactc gacgacgggg
     5461 gctcgggaat tttcgggact tcgggaccgc ctggctcgct ccagacagct ctgcaaacca
     5521 acgtgacgac tacaccgcca acctacccac cactatcttc gcactccacc caaccactcc
     5581 acccaaccca accctgacat gctcgatgcg atgagaccga gaggcgcggc caaccctccg
     5641 cccaccttcc acattcgctc gtctctcatc ccatccgcat accgggcgaa tccatccatc
     5701 caacctcgcc ggtatgcaat gggtgtcgac caccgcatct gggggccttt atacccgggg
     5761 cccgtcgcca ggcacaacgg aataaaatgc gtcgcgtttt gcgagtcggc ctcggttttt
     5821 gccggcgtgg cgaccgcgga gggccgcgtc ggcgtggcac cgcgcgctga cgacgactcg
     5881 cgcgcgatcg ttcacggcgg cggcggcggc gacggcgact ccgagcagat accgagcatc
     5941 gcctgccgct gctgcgcgca cgggtcgggg ctctcctcct cgcactcgtg gacgcaggcg
     6001 cgctcctcgg cgcagtcgta gagatcgcgg accgactgcg cacagccggc gccgaccgac
     6061 cacgcgatac cgaggtcctg gtcgcagatc gctcgcagat gcgcgtactc gtggtcggaa
     6121 caacactccg agaccgcctc cgcgtagttg ccacaaaagc cgcgactgcg ggctttgcaa
     6181 gcgagcccca gcgacacacc gacgaccgcc gccagcaacc accacgtcgc gccgcgtgct
     6241 cgtcgagcca tcaccagatg atacgccgcc ggccaccgcg gtgcagcagc acaggtagcg
     6301 catcctccac gtcgtcttcc ctccagccac gggtcgcgcg ctggcgcgct ggcgcgattc
     6361 atctacactg cgcgcgtgca cgaactgctc gacgcctccg cggtcacgct ggcccgctgg
     6421 atccgccagc gcaagctctc gccggtcgag ctgctggagg ccctgatcga ccgaatcgcc
     6481 gacgtcaacc cggcgatcaa cgccgtcatc gccgagcgct acaccctcgc cctcgccgag
     6541 gcccgcgccg ccgagcaccg cgccatgcac gagcccgcgg acaccctccc gccgctgctc
     6601 ggcctgccct acaccgccaa ggagtacatc cgcgccaccg gcatgccgct gagcgccggc
     6661 atctggtcgc gcagacatgt ccgttcggac agggacgccg agaccgtcgc ccgcctcggc
     6721 cgcgccggcg cgatcctcgt cggcatcacc aacgtgcccg agggcggcct gtggatggag
     6781 acctaccacg acgtctac
//
LOCUS       DASWNJ010001969         1885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_528898, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001969 DASWNJ010000000
VERSION     DASWNJ010001969.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528898"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..274)
                     /locus_tag="VGB85_32795"
     CDS             complement(32..274)
                     /locus_tag="VGB85_32795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138926.1"
                     /translation="MARSFYRNVLPDFTGLENETSEVNVVERDGSIGLSMTQGHGEGW
                     SRTQVFMTPEQGHELLRGLLEAIDRAERQRSLRRPN"
     gene            complement(331..>1885)
                     /locus_tag="VGB85_32800"
     CDS             complement(331..>1885)
                     /locus_tag="VGB85_32800"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0138927.1"
                     /translation="FVRPGNYLRPDEVLAVGLQLTGALAAVHAAGVVYRDLHPGNVLI
                     DRGAPTRAWIFDFDQAQVSTEFYATLTERWATPPEERTEPKHGKPLQRMDYAAPEVRA
                     GGAFTPASDVYALGLLLYRLLTGRRPFPVSGGEPTPPRTLCPACPPGLEVLLLKMLNS
                     SPEVRPGLAMVQAMLEDEQAELAAEHEDSDEPAADARLAAGEHATAPAVAADTLTAEP
                     RAGFLNDPRGREASNLAFAADRLQRDGNEPAARTHYARAAALEAEILATLAPEDSSLY
                     STLAVSAAALWFKARDPYKLDELATRLLASDALTPGARAEIAEMRAEALKGQAPDRRP
                     RRAPGWWAVGLTAVACLAIGRASVRDRPDPPTLAPLASLTDAERIEAPTPSPVHTPLA
                     LAVDNASPAAPHDPDDASPAPLHGPDPKRDAPPPTVRREAVTEAEALKAAQRAVPSLR
                     TCADVPRRVTADLDIVRGRGIVTALNLHAPDLDDPKSWHGCVVRSLKPVRFPVSQTAG
                     HVRVRLTLR"
BASE COUNT          248 a          661 c          683 g          293 t
ORIGIN      
        1 cgcgccccgc caccgcttca ggccgcacct gtcagtttgg tcgtcgtaga gagcgctgtc
       61 tctccgcgcg atcgatggct tccagcagtc cgcgaagaag ctcatggccc tgctcgggcg
      121 tcatgaacac ctgcgtacgg ctccagcctt ccccgtgccc ttgtgtcatc gacagcccga
      181 tgctgccgtc ccgctcgacg acgttgacct cgctagtctc gttctcgagg cccgtgaagt
      241 ctgggagcac gttcctgtag aagctgcgcg ccatctcggt ccccagcatg ccacatgcca
      301 gggccggtta cgcgatgctg tccgtacggt ctaccgcagg gtcagccgga cccggacatg
      361 tccggctgtc tggctgacgg ggaagcggac gggcttaaga ctccgcacca cgcaaccgtg
      421 ccaggacttc ggatcgtcca gatctggggc gtggaggttg agcgccgtga cgatgccgcg
      481 gccgcgaacg atgtcgaggt ccgcggtcac gcggcgtggg acgtcggcac acgtgcggag
      541 gcttggcacg gcgcgctgtg ccgccttcaa tgcttcggcc tcggtgacag cttcacgccg
      601 gacggtcggc gggggcgcgt cgcgcttcgg atccgggccg tgcagcggag cgggcgaagc
      661 atcgtccggg tcgtgcggcg cagcgggcga agcgttgtcc accgccaggg cgagcggcgt
      721 gtggacgggc gagggtgtag gagcctcgat ccgctcggcg tccgtcaagc tcgccagggg
      781 tgcgagcgtc ggcggatcgg gcctgtcgcg cacgctcgca cgcccgatcg ccaggcatgc
      841 gaccgcggtg agcccgaccg cccaccagcc cggagcccgc cgcgggcgcc tgtcgggcgc
      901 ctggcccttg agcgcctcgg cgcgcatctc cgcgatctcg gcgcgcgcgc cgggcgtcag
      961 cgcatcgctg gccagtaatc gcgtcgctag ctcatcgagt ttgtacgggt ctctcgcctt
     1021 gaaccacagc gccgccgcgc tgacggccag cgtgctgtag agcgagctgt cctcgggggc
     1081 cagcgtcgcc aggatctccg cctccaacgc ggccgcacgg gcgtaatgcg tgcgcgctgc
     1141 gggctcgttg ccgtcccgct gcaggcggtc ggcggcgaac gccaggttac tggcctcgcg
     1201 gccgcggggg tcgttcagga agcccgcacg gggctccgcc gtcagggtgt ccgccgcgac
     1261 ggcgggggct gtcgcgtgct cgcctgccgc gaggcgtgcg tccgcggccg gctcgtcgct
     1321 gtcctcgtgc tccgcggcga gctcggcctg ctcgtcctcc agcatagcct ggaccatggc
     1381 aagccccggg cgaacctcgg ggctcgagtt gagcattttg aggagcaaga cctcgagtcc
     1441 gggcggacat gccgggcaca gcgtccgcgg cggcgtcggc tcgccgcccg acacggggaa
     1501 gggccgccgc cccgtgagca agcggtacag caggaggccg agcgcgtaga cgtcgctggc
     1561 cggcgtgaac gctccgcccg cgcgaacctc cggcgccgcg tagtccatgc gctgcaaggg
     1621 cttcccgtgc ttcggctcgg tgcgttcctc cggcggcgtg gcccatcgct cggtgagagt
     1681 cgcgtaaaac tcggtggaca cctgcgcctg atcgaagtcg aagatccacg cgcgcgtcgg
     1741 ggccccgcga tcgatcagca cgttgcccgg gtgcaggtcg cgatacacga cgccggcggc
     1801 atgcacggcc gcgagcgccc cggtgagttg caagccgacc gccagcacct cgtcggggcg
     1861 caggtagttg ccagggcgta cgaag
//
LOCUS       DASWNJ010001970         3061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_705056, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001970 DASWNJ010000000
VERSION     DASWNJ010001970.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705056"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(746..1171)
                     /locus_tag="VGB85_32805"
     CDS             complement(746..1171)
                     /locus_tag="VGB85_32805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138928.1"
                     /translation="MCTTILLSIVTLGVAAKTHGRQIRRERERAGYTHVTFGGDNPPF
                     VTALWRRDRQRLWTTATATAFAMLALIGLGHAPASGILGTVWSLVFLPMLAGFALSGL
                     ASSRRAARSGCGSCIGGFAWWSLLSLLAGVTIISALCPC"
     gene            complement(1180..1833)
                     /locus_tag="VGB85_32810"
     CDS             complement(1180..1833)
                     /locus_tag="VGB85_32810"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY0138929.1"
                     /translation="MKIAVLGTGTVGETLADKLVSLGHHVTMGSRTRDNTKAVTWTSR
                     AGERAAQGSFADAVADAELVFLCTLGSAAVEALTAAGEDALAGKVIVDTTNPLDFSRG
                     FPPRLFVVGDDSLGEQIQRAFPRSHVVKALNTIGAPVMVEPSRVPGEHVTFLAGNDAD
                     AKTRVRAVLTEGFGWQKVIDLGDITGARATEGYMLVWLRLWGALQTPMFNIQISTGA"
     gene            1958..2974
                     /locus_tag="VGB85_32815"
     CDS             1958..2974
                     /locus_tag="VGB85_32815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138930.1"
                     /translation="MPTFTTHLSRMPVALLLATALACGSDPSGTTTGGSTTTEPGTGS
                     TQTSGSQATTGDAPTSTTDAVTSSTGSTGGATTGVTSSTADGGDTEPAGTSTDADTTG
                     AIEPGSSGSSSGTTGDGDTIAESSGDTGGTSTTGDADGLGEIMGDCGLIDAMEVESPA
                     PFVFNSAIDFGMLGYDYDLLTPGGQQIADEGGLNDGSLKSEIVAYEVLARCDMAALLK
                     TEGKIIYKDPMGKKTDLLVELADHKVGVSVVRAVGFPKDDPYTVAQADTILKKKLSDI
                     KISSANVAPEDAWIKQILSVVAYGPMHAESIVTAYEALDPTLKADTILVVSVTNGDDA
                     FIYN"
BASE COUNT          515 a          968 c         1147 g          431 t
ORIGIN      
        1 cagccggccc tacagcctaa gtgagtggca ttggggtcct cccaggggac atgctcgccg
       61 ccccatctgt cgcgcacgac agagctctgt caccgaccgt cgatcgcggg atcggaagtt
      121 gacggggcgt ctgcgcgtga cccctgggtc ctcccgctgc ttcccccgct cgtcggcccc
      181 caaccccggg cggacatgct cggcgccccg cttgtcgtca ggacgacgat ttgtcggcgg
      241 agcaagaact gccgctccgg tcatgtccat acgattggat catgattttg atgaagccag
      301 gtatccgcgc tcggccacgc ccgccacgaa tgtggccgtc ttacttggtc gcgcggacgg
      361 gcttcgcggc ggcgatggag tcgtgtgccg tgggcaagcc ggaaggcacg taggacatcg
      421 gcacgtgcga tgatatcgcg ggaggcatat ccgcgcggac atatcgtgca ttcagggccg
      481 gtgggggtcc ggctctcggc agggccgacc gaccgtcctt tagtgcaggg agcgggtggc
      541 ggaggaccga aggaggtttc gaccgagtcc gacgggcccc atgtcttcgg acgcgcgagg
      601 tgtatgcgtc gtgtttgagg gcgaaacctc cttcgggcat ccgccttctc gaggatgcac
      661 gggcgcccag ccggaccccc accggaccgt gcgacgtcgc ccgcacagca catccaacgt
      721 cactcgcaca cgcgaagccg gaaagctagc aggggcacag cgccgagata atggtgacgc
      781 cagcaaggag gctcaacagg gaccaccagg cgaagccacc gatgcacgag ccgcagcccg
      841 agcgagcagc gcggcgagag ctggcgagcc cgctgagggc gaagccggcg agcatcggca
      901 ggaacacgag gctccagacg gtgccgagga tgccgctcgc aggcgcgtgg ccgagtccga
      961 tcagggcgag catggcgaac gcggtcgcgg tggcggtggt ccacaggcgc tggcgatcgc
     1021 ggcgccacag ggcggtgacg aagggtgggt tgtcgccgcc gaaggtgacg tgggtgtagc
     1081 cggcgcgctc acgttcgcgg cggatctggc ggccgtgggt cttggcggcg acgccgaggg
     1141 tgacgatcga gagcaggatg gtggtgcaca tggggggcct caggcgccgg tggagatctg
     1201 gatgttgaac atgggggtct gcagcgcgcc ccacaggcgc agccagacga gcatgtagcc
     1261 ctcggtcgcg cgggcgccgg tgatgtcgcc gaggtcgatg actttttgcc agccgaagcc
     1321 ctcggtcagc acggcgcgca cgcgggtctt ggcgtcggcg tcgttgccgg cgaggaaggt
     1381 gacgtgctca ccgggcacgc gcgagggctc gaccatgacc ggggcgccga tcgtgttgag
     1441 ggccttcacg acgtgggacc gcgggaatgc gcgctggatc tgctcgccga gggagtcgtc
     1501 gccgacgacg aacaggcgcg gggggaagcc gcgcgagaag tcgagcgggt tggtggtgtc
     1561 gacgatgacc ttgccggcga gcgcgtcctc gccggcggcg gtcaacgcct cgacggcggc
     1621 ggacccgagg gtgcacagga acaccagctc ggcgtcggcg acggcgtcgg cgaagctgcc
     1681 ctgggccgcg cgctcgccgg cgcgggaggt ccaggtaacc gccttcgtgt tgtcgcgcgt
     1741 acgcgagccc atggtgacgt ggtggccgag agagacgagc ttgtcggcga gggtctcgcc
     1801 gacggttccg gtgccgagga ctgcgatctt catgcagcca ctgtggctgt gaaccgccgg
     1861 gacgtcgatg ggctccgtgt tgaagcatcg tcaacctggg gtcaacgatg gcacgtgttc
     1921 gcgcgcatcg cccgggtgat acagtcgggt ggccaccatg ccgaccttta cgacccacct
     1981 ctcccggatg ccggtcgcgc tgctcctggc gaccgcgctc gcgtgcggca gcgacccttc
     2041 tggcacgacg acgggcggta gcacgacgac ggagcctggc acgggcagca cgcagacctc
     2101 gggctcgcag gcgacgaccg gcgacgcgcc gacctcgacc acggacgctg tcacctcgtc
     2161 gacgggcagc acgggtggtg cgacgaccgg ggtcacgagc agcacggcgg acgggggcga
     2221 caccgagccc gcgggcacga gcaccgatgc ggacacgacc ggcgcgatcg agcccgggag
     2281 cagcgggagc agctcgggca cgaccggcga tggcgacacg atcgcggaga gcagcggaga
     2341 caccggcggc acgagcacca cgggtgacgc cgacgggctc ggcgagatca tgggtgactg
     2401 cgggctgatc gacgcgatgg aggtcgagtc gccggcgccc ttcgtgttca acagcgcgat
     2461 cgacttcggg atgctcggct acgactatga cctgctgacg ccgggtggcc agcagatcgc
     2521 ggacgagggc gggctcaacg acgggtcgct caagtcggag atcgtcgcct acgaggtgct
     2581 ggcgcgctgc gacatggcgg cgctgctcaa gaccgagggc aagatcattt acaaagatcc
     2641 gatgggcaag aagaccgacc tgctcgtcga gctcgccgac cacaaggtcg gcgtcagcgt
     2701 cgtgcgtgcg gtcggcttcc cgaaggacga cccctacacg gtcgcgcagg cggacacgat
     2761 cctcaagaag aagctgtcgg acatcaagat cagctccgcc aacgtggcgc ccgaggacgc
     2821 gtggatcaag cagatcctca gcgtggtcgc gtacgggccg atgcacgccg agagcatcgt
     2881 gacggcgtac gaggcgctcg acccgacgct caaggcggac acgatcctcg tcgtcagcgt
     2941 caccaacggg gacgacgcct ttatttacaa ctgaggccgg cgtcagcgac ccgggggcag
     3001 ggcgcggccg cgggtcgtct gctcgtggac ggggcgctgc agggtcaggc gctggccggc
     3061 g
//
LOCUS       DASWNJ010001971         2337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_345903, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001971 DASWNJ010000000
VERSION     DASWNJ010001971.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345903"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VGB85_32820"
     CDS             <1..238
                     /locus_tag="VGB85_32820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138931.1"
                     /translation="GDTATAVPILERALAIREAGGVQPEEIAETRFSLARALAPTQPQ
                     RARTLAEQALRALATSGGSAREHAEIAAWLAAHP"
     gene            complement(243..1106)
                     /locus_tag="VGB85_32825"
     CDS             complement(243..1106)
                     /locus_tag="VGB85_32825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0138932.1"
                     /translation="MTQPESSTPADPSSEVQALRAEIAALRARVNLMDAVIAASPAVM
                     FAKDIEGRLTLGNRKYEQIFPHVEGGLLGKTDLEIMGGEAAEVVRANDRRVREGGVPI
                     EFEELVPQVDGLRTYITIKFPLYDDQGACIGVAGVATDITERKRAEDDRAALQQQIIE
                     IQRATLSELSTPVIPLAEGVIAMPLVGAIDSERARQITEALLDGLASSRAHTAILDIT
                     GVRAVDSHVAGALLQSARAARLLGARVIITGIRAEVAQALVQIGTDWTGIETLSTLQS
                     GVALALSRSTR"
     gene            complement(1282..>2337)
                     /locus_tag="VGB85_32830"
     CDS             complement(1282..>2337)
                     /locus_tag="VGB85_32830"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0138933.1"
                     /translation="HYLAKPFRLDELVIFLRRAFDDTQLRREAATLRRALREQPAPPG
                     LLGHSAAMQETYRVIERVAGTAAPVLITGETGTGKSLVAAILHQRSGRASAPLVSINC
                     AALPEQLLESELFGHVRGAFSGATSESIGLFQAANGGSIFLDEIGEMPAALQAKLLHV
                     LERQTVRPIGGTRERTVDVRILSATNRDLEQAVREGKFREDLLYRLNLVALELPPLRE
                     RPADIPELLTYFLAQARARYPDSPVRGFSRDVMQKLLAHPWPGNVRELAHTLERLVLL
                     GRQETVELDDLPRNFRRGPGLSPVFDAIVPMREMQRRYASWVLEQSEGVRARAARGLG
                     IDVKTLARLLEPDGGME"
BASE COUNT          314 a          806 c          885 g          332 t
ORIGIN      
        1 gggggacacc gcgacggcgg tgccgatcct agagcgggcc ctggcgatcc gcgaggccgg
       61 cggcgtgcag ccggaggaga tcgccgagac ccgcttcagc ctcgcccgcg cgctcgcgcc
      121 gacgcagccg caacgggccc gtacgctcgc cgaacaggcg ctgcgggcac tcgcgacgtc
      181 ggggggctcg gcccgcgaac acgccgagat cgccgcctgg ctggccgcgc acccgtgagc
      241 tgctagcggg tcgagcgcga cagggcgagc gcgacgccgg attgcagggt ggagagcgtc
      301 tcgatgccgg tccagtcggt gccgatctgg acgagggcct gagcgacctc cgcccggatg
      361 ccggtgatga tcacgcgggc gccgagcaag cgggcggcgc gggccgactg gagcagcgcg
      421 ccggcgacgt gcgagtcgac cgcgcgcacg ccggtgatgt cgaggatcgc cgtgtgggcc
      481 cgtgagctgg cgaggccgtc gagcagggcc tcggtgatct ggcgcgcgcg ctcgctgtcg
      541 atcgcgccga cgagcggcat ggcgatcaca ccctcggcga gcgggatgac cggggtcgac
      601 agctcgctga gcgtcgcgcg ctggatctcg atgatctgct gctgcagcgc ggcgcggtcg
      661 tcctcggccc gcttgcgctc ggtgatgtcg gtggcgacgc cggcgacgcc gatgcacgcg
      721 ccctggtcgt cgtacagcgg gaacttgatc gtgatgtagg tcctcaggcc gtcgacctgc
      781 gggacgagct cctcgaattc gatcggcacg ccgccctcgc gcacgcgtcg gtcgttcgct
      841 cgcaccacct cggccgcctc gccgcccatg atctcgaggt ccgtcttgcc gagcaggccg
      901 ccctccacgt gcgggaagat ctgctcgtat ttgcggttgc cgagggtgag ccggccctcg
      961 atgtccttgg cgaacatcac cgccggcgag gccgcgatga cggcgtccat caggttgacg
     1021 cgggcccgca gcgccgcgat ctcggcgcgc aacgcctgga cttcggacga cgggtcggcg
     1081 ggcgtcgagg attctggctg tgtcatgtcc gtgtcttcgg gggcgccgca ccgcgggcac
     1141 ccgaccattg tagagatctt cgcgggcgag ggcgccgatg ttgccgcacc gctttgggcc
     1201 tgtctcacaa ccccgaagcg gctgtccggt ctgcacacgg ggcctgctcg atggcgcgca
     1261 tcgccccgga gccgcccgcg gtcattccat tccgccgtcc ggttcgagca agcgggcgag
     1321 ggtcttgacg tcgatgccga ggccgcgggc cgcccgggcg cgcaccccct cgctctgctc
     1381 gagcacccag gaggcgtagc gccgctgcat ctcgcgcatc ggcacgatcg cgtcgaacac
     1441 cggcgacagg ccggggccgc ggcgaaagtt gcggggtaaa tcgtcgagct cgaccgtctc
     1501 ctgacgaccc agcaacacca ggcgctcgag cgtgtgggcc agctcgcgca cgttgccagg
     1561 ccaggggtgc gccagcagct tctgcatcac gtcgcgcgag aagccgcgga cagggctgtc
     1621 cggatagcgc gcgcgcgcct gggcgaggaa gtaggtcagc agctcgggga tgtcggcggg
     1681 gcgctcgcgc agcggcggga gctcgagggc gacgaggttg aggcggtaca gcaggtcctc
     1741 gcggaacttg ccctcgcgga ccgcctgctc gaggtcgcgg ttggtggcgg acaggatgcg
     1801 gacgtcgacg gtgcgctcgc gcgtgccgcc gatcggcctg accgtctggc gctcgagcac
     1861 gtgcagcagc ttggcctgca gcgccgccgg catctcgccg atctcgtcga ggaagatgct
     1921 gccgccgttg gccgcctgga acaggccgat cgactccgag gtcgcgccgc tgaaggcgcc
     1981 acgcacgtgg ccgaacagct cgctctcgag cagctgctcg ggcagggcgg cgcagttgat
     2041 cgagaccagc ggcgccgagg cgcgaccgct gcgctggtga aggatcgcgg cgaccaggct
     2101 cttgcccgtg ccggtctcgc cggtgatcag caccggcgcg gcggtgccgg cgacccgctc
     2161 gatcacgcgg taggtctcct gcatcgccgc gctgtgcccg agcagccccg gcggcgccgg
     2221 ctgctcgcgc agcgctcgcc gcagggtcgc ggcctcgcgg cgcagctggg tgtcgtcgaa
     2281 cgcgcggcgg agaaagatga cgagctcgtc gaggcgaaac ggcttggcga ggtaatg
//
LOCUS       DASWNJ010001972         1663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_528991, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001972 DASWNJ010000000
VERSION     DASWNJ010001972.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528991"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            533..>1663
                     /locus_tag="VGB85_32835"
     CDS             533..>1663
                     /locus_tag="VGB85_32835"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0138934.1"
                     /translation="MRSLLTSSLALALTLLASPAGAAPKHSNHRCSSAFAITNVRVFD
                     GDKMIPKATVIVVDDEITAVGPKVAVPPGTPTIDGTGSTLLPGLLDSHAHAWQREELE
                     RAAQFGVTTEFDMWSELAFIQAMTEEQELDGATDRADVFSAISPATVTEGYPYNWTPD
                     IIESPTVDSPAAAKKFVKDRLKDGANHLKIMLEDGTLTGPAIPVLSNATIVALTTEAS
                     KRGMLSMAHVTRQETALAAVSSGVDGLVHVFVDALASDDFVELAVSRGIFVVGTLAAE
                     ESFITTAGGEAIIDDPDLGPYLTELEKEYLLWPPLPSVLTLENLQIAKDNVLALHDAG
                     VPILAGTDVSTHGISIHRDLELLVDAGLDPIDALVAATSAPAD"
BASE COUNT          270 a          609 c          543 g          241 t
ORIGIN      
        1 tggctccgaa ggtatcgagc ccgccgagca ccgtcggcgc gcccgagggc atcaagctca
       61 tcgtggcgat cgcgcgcggc ggaccggcca cctccgtgaa tctctcgatc gttccgcctc
      121 cgcaggcggc acaccacagg ctcacgagcg ccacgtactt catatcgccg cagtgtctcg
      181 cgggccgagg ccggcgtcct cgatgctcgc ggcggctcgg cggtccgtga tgagccggcg
      241 catccgcgcg tgctcggggt gacgagcgcc atggcgagac gtcggcgcgg atccgtcttg
      301 acaccggggt cgtcacgctc gagcttctgc gcaaatgccc gaccctcgct gcccatgatg
      361 cgctcggtct gccgaggagg cgctgcgcgg ccacgcggat cccgcacgac gatggatagc
      421 gcacggccgg gagcctccca tcgctgcaac tcgtggcatt cgcccgcgat ccggcggacg
      481 cccggcggac gctatccggg ggccacatcg ccacatacaa gggtatatta cgatgagatc
      541 tttgctcacc tcctcgctcg cgctcgccct gacattgctc gcctctccgg ccggggctgc
      601 tccgaagcat tcgaatcaca ggtgttcgag cgcgttcgcg atcaccaacg tccgcgtctt
      661 cgacggcgac aagatgatcc cgaaggcgac cgtgatcgtc gtcgacgacg agatcacggc
      721 ggtcgggccg aaggtggcgg tcccgcccgg caccccgacg atcgacggta ccggctcgac
      781 gctgctgccc ggcctgctcg acagccacgc ccacgcctgg cagcgcgagg agctcgagcg
      841 cgccgcgcag ttcggggtga cgaccgaatt cgacatgtgg tccgagctcg cgttcatcca
      901 ggcgatgacc gaggagcagg agctcgacgg ggcgaccgac cgcgccgacg tgttcagcgc
      961 gatcagcccg gcgaccgtca ccgagggcta tccctacaac tggaccccgg acatcatcga
     1021 atcgccgacc gtcgacagcc ccgcggcggc caagaagttc gtcaaggacc ggctcaagga
     1081 cggcgccaac cacctcaaga tcatgctcga ggacggcacg ctgaccggcc ccgcgatccc
     1141 cgtgctcagc aacgcgacga tcgtcgcgct gacgacggag gcgagcaagc gcggcatgct
     1201 gagcatggcg cacgtcaccc ggcaggagac cgcgctcgcc gcggtcagca gcggcgtcga
     1261 cggcctggtc cacgtgttcg tcgacgcgct ggccagcgac gacttcgtcg agctggcggt
     1321 gtcgcgcggg atcttcgtcg tcggcacgct cgcggccgag gagtccttca tcaccaccgc
     1381 gggcggcgag gcgatcatcg acgaccccga tctcggcccc tacctgaccg agctcgagaa
     1441 ggagtacctg ctgtggccgc cgctgccgag cgtgctgacg ctcgagaacc tgcagatcgc
     1501 caaagacaac gtgctcgcgc tgcacgacgc cggtgtgccg atcctcgctg gcaccgacgt
     1561 ctcgacccac gggatcagca ttcaccgcga cctcgagctg ctcgtcgacg cgggcctgga
     1621 cccgatcgac gccctcgtcg cggcgacctc ggcccccgcc gac
//
LOCUS       DASWNJ010001973         1752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_670982, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001973 DASWNJ010000000
VERSION     DASWNJ010001973.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670982"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VGB85_32840"
     CDS             complement(<1..525)
                     /locus_tag="VGB85_32840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0138935.1"
                     /translation="MSSNPFIHHGMTALITGASRGLGRALALALAPRGVRLVLVARGQ
                     AELDAVVDAARIDGGEAHGIVADIGDKQAIHAIAGEATALVGPIDLLIHNASTLGPTP
                     LRLLLDTECEDLEQVLAVNLVGPFRLSKLIAGNMALRGRGVVVHVSSDAAVEPYPRWG
                     AYGVAKAGLDHLSRI"
     gene            621..1175
                     /locus_tag="VGB85_32845"
     CDS             621..1175
                     /locus_tag="VGB85_32845"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEY0138936.1"
                     /translation="MEDVAGHLGNNIRQLREARGLTQQQMAKISGVPRPTWANLESGA
                     ANPTLAVLIKVASALQVSLEEIVSPPRTSSKFYAAADLPVRQRGKVTVRRLLPEPIAG
                     LEIERVEMPPGASMTGIPHTPGTREYLTCERGEIELGESGRVWRLAAGDVVVFRGDQS
                     HGYRNPGRETAIAYSVIALAPIAG"
     gene            complement(1179..>1752)
                     /locus_tag="VGB85_32850"
     CDS             complement(1179..>1752)
                     /locus_tag="VGB85_32850"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEY0138937.1"
                     /translation="DPSALQDALLAEHDALGSWWKVADLVGAAVTELGNRWECGRSTV
                     IEVRLASERLTRRITRLVETSPMPAPAPLALLATAAGDEHTLGLALTELVLRELGYQV
                     GWIGHDAPPDSLAAYVHERAVAVIGLSASVASCDEEVLREAVLPVADACQEVGTRLIL
                     GGRGRWPDDLPATTRVHTFAQLYVALHVAV"
BASE COUNT          271 a          570 c          678 g          233 t
ORIGIN      
        1 gatccggctc agatgatcga gcccggcctt ggcgacgcca taggcgcccc agcgcgggta
       61 gggctcgacg gcggcgtcgg agctgacgtg cacgacgacg ccgcgcccgc gcagcgccat
      121 gttgccggcg atcagcttgc tgaggcgaaa cggaccgacg aggttgacgg cgagcacctg
      181 ctcgaggtcc tcgcactcgg tgtcgagcag gaggcgcagc ggggtcggac cgagggtgct
      241 cgcgttgtgg atcaggaggt cgatcggccc gacgagcgcg gtcgcctcgc cggcgatcgc
      301 gtggatcgcc tgcttgtcac cgatgtcggc gacgatcccg tgggcctcgc cgccatcgat
      361 gcgggcagcg tcgacgacgg cgtcgagctc ggcctggccg cgcgcgacca gcacgagccg
      421 gacgcctcgc ggcgcgaggg ccagcgcgag cgcgcgcccg aggccgcggc tggccccggt
      481 gatgagggcg gtcataccat gatgaatgaa ggggttggac gacatgagcg gctcctttgg
      541 ggtacgggag cagcttgcga cctcgcggtt gatgtgtcgt cagtcttgat gaaggattgg
      601 caagcggtca tcgtggcgac atggaagacg tcgccggcca cctcgggaac aacatccgcc
      661 agctgcgtga ggcccgtggg ctcacccagc aacagatggc gaagatcagc ggcgtgccgc
      721 gaccgacatg ggcgaacctc gagtccggcg cggccaaccc gacgctcgcg gttcttatta
      781 aagtcgccag cgcgctgcaa gtgtcgctcg aggagatcgt cagcccgccg cgcacgagct
      841 cgaagttcta cgcggccgcc gacctgccgg tgcgccagcg cggcaaggtg acggtgcggc
      901 gcctgttgcc ggagccgatc gcggggctgg agatcgagcg cgtcgagatg ccgccgggcg
      961 cgagcatgac cggcatcccc cacacgccgg gcacgcgcga gtacctgacg tgcgagcgcg
     1021 gcgagatcga gctcggcgag tcggggcgcg tgtggagatt ggccgcgggc gatgtcgtgg
     1081 tgtttcgcgg cgaccagagc cacggatatc gtaatccggg gcgcgagacg gcgatcgcct
     1141 acagcgtcat cgcgctggcg ccgatcgccg gctgagcgct acacggcgac gtgcagggcg
     1201 acgtagagct gggcgaaggt gtgcacgcgg gtcgtcgccg gcaggtcgtc gggccagcga
     1261 ccgcgaccgc cgaggatcag ccgcgtgccg acctcctggc aggcgtcggc gaccgggagc
     1321 acggcctcgc gtagcacctc ttcgtcgcac gaggcgaccg aggccgagag accgatcacc
     1381 gcgactgccc gctcatggac gtaggccgcg agggagtcgg ggggcgcgtc gtggccgatc
     1441 cacccgacct ggtagccgag ctcgcgcagc accagctcgg tgagcgcgag gccgagcgtg
     1501 tgctcgtcgc ccgcggcggt cgccaggagg gccagagggg caggggcggg catcggcgag
     1561 gtctcgacga ggcgagtgat ccggcgagtg agacgctcgg aggcgaggcg cacctcgatc
     1621 acggtgctcc ggccgcactc ccagcggttg ccgagctcgg tcaccgcggc gccgacgagg
     1681 tcggcgacct tccaccagct gccgagcgcg tcgtgctcgg cgagcagcgc gtcttgcagg
     1741 gcggaaggat cg
//
LOCUS       DASWNJ010001974         4516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_346094, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001974 DASWNJ010000000
VERSION     DASWNJ010001974.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346094"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2246
                     /locus_tag="VGB85_32855"
     CDS             <1..2246
                     /locus_tag="VGB85_32855"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0138938.1"
                     /translation="AAAPSTEQLEWFRREVRLARRITHPNVARTHDMGEQAGAHYITM
                     EFIEGTTLHELLGEDTQGPGRRQALALVQAVRIAAAVCDGLAAAHAAGVVHRDLKPAN
                     VLIERGGRVVLTDFGIARALSDEQAGARTQGVVGTPQYMAPEQLSGTPVDARADVYAL
                     GLMLFEMVTGALPFAGGTPLASALARLTERPPDPRTLRSGLPDALATLILECLATAPN
                     DRPPTVMAVGERLRSWLVAEGATLPGVQAPGESGSSNPITPPGTSRTLSRPQRPLVPM
                     MQLTLTQRALAVLPFRHIGPAEQSYLGDALTDELIDVLSRTRGLRVLGSGATAKYRTD
                     RDPRAVGADLGVDAVVDATVHSTATHLRVAVRLVEVASGTQLWSERFESGLEDLFEVQ
                     DRISRRIAEALRVELGSAASMGDVPAGAVELYLRGRRQLISFRILGPDGAIDLLEQSL
                     ELAPDMQPALACYAIACTRAWFVPPPPDPRPSDLRNWEAVAKESVARAVEHAPEQAET
                     HLARAMLAMQLGEGREAVQALVKALDIAPTYAHAHQYLAQLQCEAGNAKEGIERARLA
                     HDLEPSLLGALMDVARLQALRGDREACERTLAIVERDSPTRRMTTTFRVRLAAWSGDF
                     DQVRRLTEEIQAEGLEGFASFVVGFAQAIAGLTPREGVDRMVASLIADARSPRFLTLM
                     YQLCTEIYAALGAHQTALTYFLRAADCVLIDIEWADRCPLLAGLRALPEYVQGRQTVR
                     RRVQAMW"
     gene            complement(2251..2919)
                     /locus_tag="VGB85_32860"
     CDS             complement(2251..2919)
                     /locus_tag="VGB85_32860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138939.1"
                     /translation="MKTAPPGLVRRPREILATLNSEAGDHAVVTDPRELQAAIRAGER
                     SWQRFPYYEQRYAERGEKFTRSDSAWLASLADYGQSTATSQIIWLGRVLASRGMPRWL
                     LELHLGVLHDELSRALPARASIYATLLGAADTLRALREEHIPAALFTGLTTSFAEQVG
                     PEWDSRLPETGGLLAAAVADEKAGIALAVPSLAIWMTDASRFPPRWISAVEATIERAR
                     DHSP"
     gene            3109..3273
                     /locus_tag="VGB85_32865"
     CDS             3109..3273
                     /locus_tag="VGB85_32865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138940.1"
                     /translation="MESTTDRIERAMVPRDPRARVWRAPADAEAFGAWFGVRLEGPFV
                     PGQRYVRAAA"
     gene            3254..3610
                     /locus_tag="VGB85_32870"
     CDS             3254..3610
                     /locus_tag="VGB85_32870"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY0138941.1"
                     /translation="MSARRPDSAALAGPVDAAPVFAALGDETRIRMVARLGAGGPLST
                     AQLGEGTGVTRQAVTRHLHVLADAGLVRATRLGRDSIWELESSRLAEARRVLQSISEQ
                     WDGALQRLRAFVEDGP"
     gene            complement(3664..>4516)
                     /locus_tag="VGB85_32875"
     CDS             complement(3664..>4516)
                     /locus_tag="VGB85_32875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138942.1"
                     /translation="VGYTDPALFEGHPAQVQSDIFSVAVVLYEMLAGRRLHDEKAGRF
                     QAIDSGEFAPELAPVVAEIRRGSERLPKDRHASMDEFIRGLEIARSMVLRAKVSPPRG
                     RSLSGLGWASLGAMAMLGVFWASGRPIGTADGSAKIEPAAEVTQARPADDTTAAGTVE
                     PADVTPSRPAEVVPVAHAAEVEPASEKGPMPRTSAKAQAASASRGIADPMNAVGAAAG
                     ACLTTPDPVDLEIMVTAERAVLKRVAWVPYNPGDATESCIARAIGSVQFPKSYPAGSF
                     RLEVRGR"
BASE COUNT          627 a         1512 c         1722 g          655 t
ORIGIN      
        1 ccgcggcggc gccgtcgacc gagcagctcg agtggttccg ccgcgaggtg cgcctggccc
       61 ggcgcatcac gcacccgaac gtcgcgcgca cgcacgacat gggtgaacag gcgggcgcgc
      121 attacatcac gatggagttc atcgagggta cgacgctgca cgagctgctc ggcgaggaca
      181 cgcaggggcc gggacggcgg caggcgctcg cgctggtgca ggcggtccgc atcgccgcgg
      241 cggtgtgtga cgggctcgcg gcggcgcacg cggcgggcgt ggtgcatcgc gacctcaagc
      301 cggccaacgt gctgatcgag cgcggcggtc gggtggtatt gacggacttc ggcatcgcgc
      361 gggcgctgag cgacgagcag gccggcgcgc gcacgcaggg ggtggtcggc acgccgcagt
      421 acatggcgcc ggagcagctc tcgggcacgc cggtcgacgc tcgcgccgat gtatatgcgc
      481 tgggtttgat gctgttcgag atggtgacgg gggcgctgcc attcgccggc ggcacgccgc
      541 tggcgtcggc gctggcgcgg ctgaccgagc gcccgccgga cccgcggacg ctgcgctcgg
      601 ggctgcccga cgcgctggcg acgctgatcc tcgagtgtct ggcgacggcg ccgaacgacc
      661 ggccaccgac ggtgatggcg gtcggggagc ggctgcggtc ctggctggtg gccgagggcg
      721 cgacgctgcc gggagtgcag gcgccgggcg agtccggctc gtcgaacccg atcacgccgc
      781 cggggacgtc gcggacgctg tcgcgtccgc agcggccgct ggtgccgatg atgcagctga
      841 cgctgacgca gcgcgcgctg gcggtgctgc cgtttcgcca catcggtccg gcggagcaga
      901 gctacctcgg cgacgcgctg accgacgagc tgatcgatgt attgtcgcgg acccgcgggc
      961 tgcgggtgct cggcagcggc gcgacggcca agtatcgcac cgaccgcgac ccgcgggcgg
     1021 tcggcgcgga cctcggggtc gacgcggtgg tcgacgcgac ggtgcacagc acggcgacgc
     1081 acctgcgggt cgcggtgcgc ctggtcgagg tggcgagcgg gacgcagctg tggagcgagc
     1141 gcttcgagag cgggctcgag gatctgttcg aggtccagga ccggatctcg cgccggatcg
     1201 cggaggcgct gcgcgtcgag ctcggctcgg cggccagcat gggcgacgtg ccggccgggg
     1261 cggtcgagct gtacctgcgg ggccgccggc agctgatctc gtttcgcatc ctcggcccgg
     1321 acggcgcgat cgacctgctc gagcagagcc tggagctcgc gccggacatg cagccggcgc
     1381 tcgcgtgtta cgcgatcgcg tgcacgcgcg cgtggttcgt gccccctccg cccgacccgc
     1441 gtccctcgga cctgcgaaac tgggaggcgg tcgccaagga gagcgtcgcg cgggccgtcg
     1501 agcacgcgcc cgagcaggcc gagacccacc tcgcccgcgc gatgctggcg atgcagctcg
     1561 gcgagggccg cgaggcggtg caggcgctgg tcaaggccct cgacatcgcg ccgacctacg
     1621 cgcacgcgca tcagtacctc gcgcagctgc agtgcgaggc gggcaacgcg aaggagggga
     1681 tcgagcgggc gcgcctggcc cacgacctcg agccgagcct gctcggggcg ctgatggacg
     1741 tcgcgcgctt gcaggcgctg cgcggggacc gtgaggcgtg cgagcggacc ctggcgatcg
     1801 tcgagcgcga cagcccgacc cgccgcatga ccacgacgtt tcgcgtccgc ctggccgcgt
     1861 ggtcggggga cttcgaccag gtgcgccgtc tgacggagga gatccaggcg gaggggctcg
     1921 agggcttcgc cagcttcgtc gtcggcttcg cccaggcgat cgccgggctg acgccgcgcg
     1981 agggggtcga ccgcatggtc gcgtcgttga tcgccgacgc ccgcagcccg cgatttttga
     2041 cgctgatgta tcagctgtgc accgagatct acgcggcgct cggcgcccac cagacggcgc
     2101 tgacatactt cctgcgcgcg gccgactgcg tgctgatcga catcgagtgg gccgaccgct
     2161 gcccgctgct cgccgggctg cgcgcgctgc cggagtacgt gcagggccgc cagacggtgc
     2221 gcaggcgcgt gcaggcgatg tggtgagcgc tcagggcgag tgatcccgcg cgcgctcgat
     2281 cgtcgcctcg acggcggaga tccagcgcgg cgggaagcgt gaggcgtcgg tcatccagat
     2341 cgccaggctc ggcacggcga gggcgatgcc cgccttctcg tcggcgaccg ccgcggccag
     2401 cagcccgccg gtctccggta accgtgagtc ccactcgggc ccgacctgct cggcgaagct
     2461 cgtcgtcagc ccggtgaaca gcgcggcggg gatgtgctcc tctcgcagcg cgcgcagcgt
     2521 gtcggcggcg ccgagcagcg tcgcgtagat gctggccctg gccggcaggg cgcggctcag
     2581 ctcgtcgtgc aggaccccga ggtgcagctc gagcagccag cgcggcatgc cacgcgaggc
     2641 gagcacgcgg ccgagccaga tgatctggga ggtggcggtc gattgcccgt agtctgccag
     2701 cgaggcgagc caggcgctgt cgctgcgcgt gaacttctcg ccgcgctccg cgtagcgctg
     2761 ctcgtagtag gggaagcgct gccacgagcg ctcaccggcg cggatcgccg cctggagctc
     2821 gcgcgggtcg gtgaccacgg cgtggtcacc ggcctcggag ttgagggtcg cgaggatctc
     2881 acgcggccgt cgtaccagcc cgggcggtgc ggtcttcatg ctccccacga tatacagtca
     2941 ggcggtcgat cggcggacga ttacatcggg atcttcacgg agcgtgatcc acgatatctg
     3001 catcgggtga gccgcggaac cggctgttcc gtccatccct gggcgattga caacgttttt
     3061 gttgcgcgtt tggaagtggc ggtgtaacgg acaaccagga ggttgcgcat ggaatccacg
     3121 acggaccgca tcgaaagagc gatggtgcca cgtgacccgc gggcgcgggt gtggcgggcg
     3181 ccggccgacg cggaggcgtt cggcgcgtgg ttcggggtgc ggctcgaggg gccgttcgtg
     3241 ccggggcagc ggtatgtccg cgcggcggcc tgacagcgcg gccctggcgg ggcccgtcga
     3301 cgcggcgccg gtgttcgcgg cgctcggcga cgagacgcgc attcgcatgg tcgcccgcct
     3361 cggcgcgggc gggccgctgt cgacggcgca gctcggcgag ggcaccgggg tgacgcgtca
     3421 ggcggtgacg cggcacctgc acgtgctcgc cgacgccggc ctggtccgcg cgacgcggct
     3481 cggccgcgac agcatctggg agctcgagtc gtcccggctc gcagaggccc gccgggtgtt
     3541 gcagtcgatc tccgagcaat gggacggggc gctccagcgc ctgcgtgcgt tcgtcgagga
     3601 cgggccgtga aaacggcgag ggaggtcgat cgacgagttg atccgcgaaa cgcaagcggc
     3661 cgtttaacgc ccgcggacct cgagtcggaa agatcctgcg gggtaggatt tcgggaattg
     3721 gacggagcca atggcccgag caatacagct ctccgttgcg tctcccgggt tgtaaggcac
     3781 ccatgcaacg cgctttagca cggcgcgctc cgctgtcacc atgatctcga gatcgacggg
     3841 gtccggtgtc gtgaggcagg cccccgccgc tgcaccgacc gcattcatcg gatcggcaat
     3901 gccgcggctc gcggaggccg cctgcgcttt tgcgcttgtg cgcggcatgg gtcctttctc
     3961 ggaagcgggc tcgacctcgg cggcgtgggc gaccggcacg acctcggcgg gccgggatgg
     4021 cgtgacgtcg gcgggctcga ctgtacccgc cgcggtcgtg tcatcggcgg gccgagcttg
     4081 cgtgacctcg gcggcgggct cgattttagc ggatccatcc gcagtcccga tcgggcgtcc
     4141 ggaggcccaa aagaccccga gcatcgccat cgccccgaga cttgcccagc caagaccaga
     4201 cagacttcgc cctcgcggtg ggctgacctt cgcgcggagg accatggacc gggcgatctc
     4261 caacccccgg ataaactcgt ccatgctggc atgacggtct ttaggtagac gttcgctccc
     4321 ccggcgaatc tcggcgacta ccggcgccag ctccggagcg aactcaccgc tatcgatggc
     4381 ctgaaaccgc ccggccttct cgtcgtgaag gcgtcgcccc gccaacattt cataaagtac
     4441 gacggccacc gaaaaaatgt cgctttgaac ctgggcaggg tggccctcga agagcgcggg
     4501 atcggtataa cccacg
//
LOCUS       DASWNJ010001975         1897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_70455, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001975 DASWNJ010000000
VERSION     DASWNJ010001975.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70455"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1417)
                     /locus_tag="VGB85_32880"
     CDS             complement(50..1417)
                     /locus_tag="VGB85_32880"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0138943.1"
                     /translation="MATPSTPGLTQELPLPPGTHGLPLIGETLEFLRSPHRFADSRIA
                     RFGKVYQSHLLGKPTIFLIGAEANRWIFAGEGRYLENEWSPAIRKLLGASSLALITGE
                     AHKQRRKLLAPHFKRTAMAGSVAPMVRIARAHLDRWAEDAGRGALAVVPRMRALVFEI
                     AAIYLLGDIDDLGVGLDEFSRDFDTWVAGLFAVVPLALPYTRFGRAMQARGRLFGVLE
                     RLVARRDREARDDDSVLSTLLRVRDEDGRPLPRDTIVDEIQLLLFAGHDTTVTSTTNI
                     LYHLAVHPEAQARARAEQDAMATREYTLESLRAMPWLDAVARESMRLIPPIGGAFRVM
                     TQDGEFAGFRIPRGHRIAVGPRATHREAEHWPDPDRFDPERFLREADDRPAFAWIPFG
                     GGPRVCLGQHFAMLEMMVVVAMLLREFEWTLTPGQDPTFEVIPLPRQRSGTLIDLRLR
                     APPGP"
     gene            1713..>1897
                     /locus_tag="VGB85_32885"
     CDS             1713..>1897
                     /locus_tag="VGB85_32885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138944.1"
                     /translation="MRTSLFVALAFVACAPRQYHAGSDDILVRDRHTTRTVKIRGSVL
                     RRNTEEPLAGARVLIFMT"
BASE COUNT          278 a          645 c          701 g          273 t
ORIGIN      
        1 cggaccccgc tgcatgtcgg cgcctccgcc catgcagata tcggctcgct cagggtccag
       61 gcggcgcgcg gaggcgcagg tcgatcagcg tcccgctgcg ctgtcgtggc agcgggatga
      121 cctcgaaggt cgggtcctgg ccgggggtga gcgtccattc gaattcacgc aggagcatgg
      181 cgaccacgac catcatctcg agcatcgcga agtgttgccc gaggcagacc cgcggaccgc
      241 cgccgaaggg gatccacgcg aaggccgggc ggtcgtcggc ctctcgcagg aagcgctcgg
      301 ggtcgaagcg gtcggggtct ggccagtgct cggcctcgcg gtgcgtcgcg cggggaccga
      361 cggcgatccg gtgaccgcgg ggtatgcgaa atccggcgaa ttcgccgtcc tgcgtcatga
      421 cgcgaaacgc cccgccgatc ggcgggatca ggcgcatgct ctcgcgggcc accgcgtcga
      481 gccagggcat cgcgcgcagg ctctccagcg tgtattcacg ggtcgccatc gcgtcctgct
      541 cggcccgcgc gcgcgcctgc gcctcggggt gcacggcgag gtgatacagg atgttggtgg
      601 tgctggtgac ggtcgtgtcg tggccggcga acagcagcag ctggatctcg tcgacgatcg
      661 tgtcgcgggg cagcgggcgg ccgtcctcgt cgcgcacgcg cagcagcgtc gagagcacgc
      721 tgtcgtcgtc gcgcgcctcg cgatcgcgcc gggcgacgag ccgctcgagc acgccgaaca
      781 gccgcccgcg cgcctgcatc gcccggccaa agcgcgtgta gggcagcgcc agcggcacga
      841 cggcgaacag gccggcgacc caggtgtcga agtcgcggct gaactcgtcg aggccgacgc
      901 cgaggtcgtc gatgtcaccg agcaggtaga tcgcggcgat ctcgaacacc agcgcgcgca
      961 tgcgcggcac cacggccagc gcgccacgcc cggcgtcctc ggcccagcgg tcgaggtgcg
     1021 cgcgggcgat ccggaccatc ggcgcgacgc tgccggccat cgcggtgcgc ttgaagtgcg
     1081 gggccagcag cttgcgccgc tgcttgtgcg cctcgccggt gatcaacgcc aggctgctcg
     1141 cgccgagcag cttgcggatc gcaggcgacc actcgttctc gaggtagcgg ccctcccccg
     1201 cgaagatcca ccggttggcc tccgccccga tcaggaagat cgtcggcttg ccgagcaggt
     1261 gcgactgata caccttgccg aagcgcgcga tccggctgtc cgcgaagcgg tgcggggagc
     1321 ggagaaattc gagggtctcg ccgatcagcg gcaagccgtg cgtgcccgga ggcagcggca
     1381 gctcctgtgt caggccgggt gtggaggggg tcgccatcgc cggggctttc tatcacgggg
     1441 tcgctcactt cgcgctgacc cgggtgcggc atccgtgccg gacgcacgag cagcgcagcg
     1501 atcgccggga gtggcggcgg cgggccgcgg tttacattcg ccgatctgga gagcaagcgc
     1561 ctcgaggatt tcacgcgcga cggcgacaag ctcggccgcc agctggtcga gctgcagagc
     1621 aagcggctgg tgaagaagat cgagctcgag gatatgctgc agaacctcga cctgagcttc
     1681 ccggcaccgt gaacaccggg taggcttcgg gggtgcggac ctcgctcttc gtggccctgg
     1741 cgttcgtcgc gtgcgccccg cgccagtatc acgcggggtc cgacgacatc ctggtccgcg
     1801 accggcacac gacccgcact gtgaagatcc gcgggtccgt cctccgccgg aacacggagg
     1861 agccgctcgc gggggcgcgg gtcctgatct tcatgac
//
LOCUS       DASWNJ010001976         3284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_346246, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001976 DASWNJ010000000
VERSION     DASWNJ010001976.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346246"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..2262
                     /locus_tag="VGB85_32890"
     CDS             94..2262
                     /locus_tag="VGB85_32890"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEY0138945.1"
                     /translation="MVKSAEATLASVCDKLARSYSPEAAALLERAHAAVVAVDPQAGP
                     GGVEACDLLADLRIDPPALAAAMVAELAIDGRLGLDTIREQLGEDTQQLVDGVLRLAT
                     FRWDRLDADVAESLRKMFIAVAADVRVVIVALALRLRDMRGLRRRADDVRRKIARETL
                     EIYAPLANRLGIFQLKWELEDLALRELEPEIYREIGAALAARRDERTGYITEAITTIE
                     QKLAESGIAGKISGRPKHIYSIYKKMQRKRVGFDEIYDVSAVRVLVDTIPSCYAVLGI
                     VHSLWTPISGEFDDYIAKPKANGYQSLHTAIVGPGGRPLEVQIRTQDMHTFGEYGVAA
                     HWAYKESRKAGKLADAKFNWLRQLMDWQKEVTDPKEFVESLKTDIFQDQVYVFTPGGD
                     VIDLPLGATPVDFAYRVHTNVGHRCRGALVNGQIVPLDHKLATGDRVEILTHKAQQPS
                     RDWINPQLGYVVTSGARQKIRQWFRQQGREAAIQQGRDSLDRELRRLGIDRPRHEDYV
                     ALFPKIGSVDDLLASIGFGDIATSTVATRILDAERAHAAAEAREEAARLAAVQAAAPD
                     ARKSAPRPSAAGGVSIEGTDGVLSHPARCCTPVPGDAVIGYITRGRGIAIHRRDCPNI
                     VSSPEPERLIDISWGQNRSRYPTTVAIQAADRVGLLRDISEVFADLGVSMSQTTVTTN
                     RKDQTAMITAVLEVSNNDQLVRILGRIERVPSVIKARRQR"
     gene            complement(2240..3061)
                     /locus_tag="VGB85_32895"
     CDS             complement(2240..3061)
                     /locus_tag="VGB85_32895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0138946.1"
                     /translation="MTDDALAPTLSLQGVLDALADPIFVKDRALRFTGCNEALCALLG
                     RTREEIVGRTDPEMFPPDQVEVFRSVDERILATGEPFRVEEWLSTAAGLRRIMTRKFP
                     LRDDAGAVSGLCGVITDITEQHERQLAADQVVADQRDRLAQLSVPVLQIWDGTLLLPL
                     IGELDDERAAQVRDSLLVAVNAQRARVVLIDVTGLLELGPSTAQHLLAAVRGCSLLGC
                     RSILVGVRPELALALVTGAVDLHDLPTRLTLQEGLATALAWLGARVSAFTAGDGP"
     gene            complement(3072..>3284)
                     /locus_tag="VGB85_32900"
     CDS             complement(3072..>3284)
                     /locus_tag="VGB85_32900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138947.1"
                     /translation="RLVFPADDTSRFRGTLSTTRQGTLLSLHQVNERTGYQAMYIGKV
                     VDESRIAGIFTDFAGPDGDFRLRRVR"
BASE COUNT          528 a         1191 c         1136 g          429 t
ORIGIN      
        1 gcggtcggcg ccggcgaacg gcccggccgg cgggcgcgac tgtagcgcgc gatcgaccgc
       61 gcaggcgcgc cggtgctagg ctcgcgcccg cccatggtca agtctgccga agcgaccctc
      121 gccagcgtct gcgacaagct cgcccgcagc tactcccccg aggccgccgc cctgctcgag
      181 cgcgcgcacg cggccgtggt cgccgtcgac ccccaggcgg gccccggcgg cgtcgaggcc
      241 tgcgacctgc tcgccgacct gcgcatcgac ccgcccgcgc tcgcggccgc gatggtcgcc
      301 gagctggcga tcgacggccg cctcggcctc gacacgatcc gcgagcagct cggcgaggac
      361 acccagcagc tcgtcgacgg cgtgctgcgc ctggcgacct tccgctggga ccgcctcgac
      421 gccgacgtcg ccgagagcct gcgcaagatg ttcatcgccg tcgcggcgga cgtgcgcgtc
      481 gtcatcgtcg cgctcgcgct gcgcctgcgg gacatgcgag ggctgcggcg ccgcgcggac
      541 gacgtccgcc gcaagatcgc ccgcgagacc ctcgagatct acgcgccgct ggccaaccgc
      601 ctcggcatct tccagctcaa gtgggagctc gaggacctcg cgctgcgcga gctggagccg
      661 gagatctacc gcgagatcgg cgcggcgctg gcggcccgcc gcgacgagcg caccggctac
      721 atcaccgagg cgatcaccac gatcgagcaa aagctcgccg agtccgggat cgccggcaag
      781 atcagcggcc gccccaagca catctacagc atctataaaa agatgcaacg caagcgcgtc
      841 ggcttcgacg agatctacga cgtcagcgcc gtgcgcgtgc tcgtcgacac gatcccgagc
      901 tgttatgcgg tgctcggtat tgtgcacagt ttatggacgc cgatctcggg cgagttcgac
      961 gattacatcg ccaagcccaa ggccaacggt tatcaatcgc tgcacaccgc gatcgtcggc
     1021 ccgggcggcc gcccgctcga ggtccagatc cgcacgcagg acatgcacac cttcggcgag
     1081 tacggcgtcg ccgcgcactg ggcctacaag gagagccgca aggccggcaa gctcgcggac
     1141 gccaagttca actggttgcg ccagctgatg gactggcaaa aggaggtcac cgaccccaag
     1201 gaattcgtcg agtcgctcaa gaccgacatc tttcaggatc aggtctatgt attcacgccg
     1261 ggcggcgacg tgatcgacct gccgctcggc gcgacgccgg tcgacttcgc gtatcgcgtg
     1321 cacaccaacg tcgggcaccg ctgccgcggc gcgctggtca acggccagat cgtgccactg
     1381 gaccacaagc tggcgaccgg cgaccgcgtc gagatcctga cccacaaggc ccagcagccg
     1441 agccgcgact ggatcaaccc gcagctcggt tatgtggtca cctcgggcgc gcgccagaag
     1501 atccggcagt ggttccgcca gcagggccgc gaggcggcga tccagcaggg cagggactcg
     1561 ctcgaccgcg agcttcgccg cctcggcatc gaccgcccgc gtcacgagga ctacgtcgcc
     1621 ctgttcccga agatcggcag cgtcgacgac ctgctcgcgt cgatcggctt tggtgacatc
     1681 gccacctcca cggtagcgac ccgcatcctc gacgccgagc gcgcccacgc ggccgccgag
     1741 gccagggagg aggccgcccg cctcgccgcg gtccaggccg cggcccccga cgcccggaag
     1801 tccgcgccgc gcccctcggc cgccggcggc gtcagcatcg agggcaccga cggcgtgctc
     1861 agccacccgg cgcgctgctg cacgccggtg cccggcgacg ccgtcatcgg ctacatcacc
     1921 cgcggccgcg ggatcgccat ccaccgccgc gactgcccca acatcgtcag cagcccggag
     1981 cccgagcgcc tgatcgacat ctcgtggggc caaaatcgca gccgctaccc gacgaccgtc
     2041 gccatccagg cggccgaccg cgtcggcctg ctgcgcgaca tcagcgaggt gttcgccgac
     2101 ctcggcgtca gcatgtcgca gacgaccgtc accaccaacc gcaaggacca gaccgcgatg
     2161 atcaccgcgg tcctcgaggt ctccaataat gaccagctgg tgcgcatcct cggccggatc
     2221 gagcgggtgc cgagcgtgat caaggcccgt cgccagcggt gaacgccgag acgcgcgcgc
     2281 cgagccacgc gagcgccgtc gccagtcctt cttgcagggt gaggcgggtc gggaggtcgt
     2341 ggaggtcgac cgcgccggtg acgagcgcga gcgcgagctc ggggcggacg ccgacgagga
     2401 tgctgcggca gccgagcagg ctgcagccgc ggacggcggc gagcaggtgc tgggcggtcg
     2461 acggtccgag ctcgagcaat cccgtgacgt cgatcagcac gacgcgggca cgctgggcat
     2521 tgacggcgac gagcaggctg tcgcgcacct gggccgcgcg ctcgtcgtcg agctcgccga
     2581 tcaagggcag cagaagggtg ccgtcccaga tctgcagcac ggggaccgag agctgcgcga
     2641 ggcggtcgcg ctggtccgcg accacctggt cggcggcgag ctggcgctcg tgctgctcgg
     2701 tgatatcggt gatcacgccg cacagcccgg acacggcgcc ggcgtcgtcg cgcagcggga
     2761 acttgcgcgt catgatccgc cgcagcccgg cggcggtgct cagccactcc tcgacgcgaa
     2821 acggctcgcc ggtggcgagg atacgctcgt cgacggagcg gaacacctcg acctgatccg
     2881 gcgggaacat ctccgggtcg gtgcgcccga cgatttcctc gcgcgtccgg ccgagcagcg
     2941 cgcacagggc ctcgttgcag ccggtgaagc gcagcgcccg gtccttgacg aagatcgggt
     3001 cggcgagcgc gtcgagcaca ccctgcagcg agagcgtcgg ggcgagcgcg tcgtcggtca
     3061 tgaccgacct cctaccggac gcgtcgcagg cgaaagtctc cgtcgggtcc ggcgaagtcg
     3121 gtgaagatcc cggcgatgcg cgactcgtcg acgaccttgc cgatatacat cgcctggtag
     3181 ccggtgcgct cgttgacctg atgcagcgac agcagcgtgc cctgacgggt ggtgctgagg
     3241 gtgccgcgga agcggctggt gtcatcggcg gggaacacga ggcg
//
LOCUS       DASWNJ010001977         3434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_529378, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001977 DASWNJ010000000
VERSION     DASWNJ010001977.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529378"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VGB85_32905"
     CDS             complement(<1..261)
                     /locus_tag="VGB85_32905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138948.1"
                     /translation="MFSGKTLFDSVLPVLYVGGLLTVGVAMSLLLRRVATVAAFRQLH
                     PWIPVLHVAVWGPILFILEGRLVPDFDGPAALGRALGFLVLAI"
     gene            358..996
                     /locus_tag="VGB85_32910"
     CDS             358..996
                     /locus_tag="VGB85_32910"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEY0138949.1"
                     /translation="MGLQDLIRWILPREDVFYGLIERQSEKLDEAAKALAQFTRGVPA
                     EQIREQVQDIEHQADAIVYEIEEQFARVFVTPIDREDLQLLAASLDDVVDLINLTART
                     FVLFGVPEPTPPMTELMLILVKMGEVFRAELPALRKHDYAALIAAGRVIKEHEKEADR
                     VFRNALGQLFHDPNIDAKVLLRDKEVLEDLERAIDKCETVAERLKNLAVKHG"
     gene            998..2032
                     /locus_tag="VGB85_32915"
     CDS             998..2032
                     /locus_tag="VGB85_32915"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY0138950.1"
                     /translation="MLTLLILVVVVALIFDYINGFHDAANAIATVVSTGVLPVRTAVM
                     LAGVLNFVGAITGTAVAKTIASGFADPMVVTQVVVLAALIGACIWNLLTWWYGIPSSS
                     SHALIGGLAGAVVAHSGVGAFNWNTLVHKVLIPLVASPLIGFTVALFFMVALLWTVRR
                     MRPASIQRNSRRLQLLSACAMAFSHGSNDAQKAMGIITLALVSFIAAGHVGIPAAMMP
                     PDGNGVPTWVIFSCAAAIGLGTMAGGKRIIKTMGSKIIRITPLQGFAAETAGTATILT
                     ASALGVPVSTTHCISACIMGVGASKRLSAVRWGVAGNIVLAWVLTIPMSAGFAYLALW
                     GLRGIFGPEA"
     gene            2235..3341
                     /locus_tag="VGB85_32920"
     CDS             2235..3341
                     /locus_tag="VGB85_32920"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY0138951.1"
                     /translation="MESSFKAHGAPFAVVGFTDDLRVRAWSGRAEQIFGRTEADALGG
                     NVAELVPVDGGEARWRELLSDDDGSTACLQHADGRVWEWTLTMVRDGEVRGVVCYGRD
                     VTERVAEKNQAELDRVMLHAILDNLPVVAVVLDKDGTYQMMEGKGLEGVGVKPGQMVG
                     LNALEVLKDNPEVVEYLRRGLAGIQAPPSTLFEYGRYMESWHIPLPPGGRSRMVSISL
                     DVSESRLREMELLEKLDLIERQQRVIRELSTPIIEVWEGVVALPIIGLVDSMRTSDIM
                     DSLLQTVVRLRARHAILDMTGVDVVDTATANHLIGMIRAVRLLGAEGILTGIHPGIAQ
                     TIVTLGVDLRGITVHATLRQALVYCIGRARKTKA"
BASE COUNT          538 a         1131 c         1227 g          538 t
ORIGIN      
        1 gatcgccagc acgaggaagc cgagggcgcg cccgagggcc gccgggccgt cgaagtcggg
       61 gaccagccgg ccctcgagaa tgaagaggat cggtccccac acggccacgt gcaggacggg
      121 gatccacggg tgcagctggc ggaaggccgc gacggtggcg acgcgccgca gcagcaggct
      181 catggccact ccgaccgtca gcagaccgcc gacgtagagg accggcagca ccgagtcgaa
      241 cagggtcttg ccgctgaaca tgtcacgaga atgacacagt tctcgcgcgg tgcccgcgtc
      301 cggcgggatg gacagcggcg gcgggggcgg ttagggtccg gccgcggaga atcgcccatg
      361 ggcctgcaag acctcattcg atggatcctt ccccgagagg acgtgtttta cggcctcatc
      421 gaacgccaga gcgagaagct cgacgaggcc gccaaggcgc tggcgcagtt cacccgcggg
      481 gtgcccgcag agcagatccg ggagcaggtg caggacatcg agcaccaggc cgacgcgatc
      541 gtctacgaga tcgaggagca gttcgcgcgg gtgttcgtca cgccgatcga ccgtgaggac
      601 ctccagctgc tcgccgcgtc gctcgacgac gtcgtcgacc tgatcaacct gacggcgcgc
      661 accttcgtgc tgttcggcgt gccggagccg acgccgccga tgaccgagct gatgctgatc
      721 ctcgtcaaga tgggcgaggt gtttcgcgcc gagctgccgg ccctgcgcaa gcacgactac
      781 gcggccctga tcgccgccgg acgcgtgatc aaggagcacg agaaggaggc cgaccgggtg
      841 ttccgcaacg ccctcggcca gctgttccac gaccccaaca tcgacgccaa ggtcctgctg
      901 cgcgacaagg aggtgctgga ggacctcgag cgcgcgatcg acaagtgcga gacggtcgcc
      961 gagcggctca agaacctggc ggtgaagcat ggttagcatg ctgacgctgc tgatcctcgt
     1021 cgtcgtcgtc gcgctgatct tcgactacat caacggcttc cacgacgccg ccaacgcgat
     1081 cgccacggtg gtctcgaccg gcgtgctgcc ggtgcgcacc gcggtgatgc tcgccggcgt
     1141 gctcaacttc gtcggcgcga tcaccggcac ggcggtcgcc aagacgatcg cctcgggctt
     1201 cgcggacccg atggtcgtca cccaggtggt cgtgctcgcg gcgttgatcg gcgcgtgcat
     1261 ctggaacctg ctgacctggt ggtacggcat cccgtcgagc tcgtcgcacg cgctgatcgg
     1321 cgggctcgcc ggcgcggtgg tcgctcacag cggcgtcggc gcgttcaact ggaacacgct
     1381 ggtccacaag gtcctgatcc cgctcgtcgc ctcgccgctg atcggcttca ccgtcgccct
     1441 gttcttcatg gtcgccctgc tgtggacggt gcgccgcatg cgaccggcga gcatccagcg
     1501 caactcgcgg agattgcagc tgctgtccgc ctgcgcgatg gccttctcgc acgggtccaa
     1561 cgacgcccag aaggcgatgg gcatcatcac cctcgcgctg gtgtcgttca tcgccgcggg
     1621 tcacgtcggc atcccggcgg cgatgatgcc ccccgacggc aacggggtcc cgacatgggt
     1681 catcttctcc tgcgccgcgg cgatcggcct cggcacgatg gccggcggca agcgcatcat
     1741 caagacgatg ggctcgaaga tcatccgcat cacgccgctg cagggcttcg ccgccgagac
     1801 cgccggcacc gcgacgatcc tgaccgccag cgcgctcggc gtgccggtct cgacgaccca
     1861 ctgcatcagc gcctgcatca tgggcgtcgg cgccagcaag cgcctgtccg cggtccgctg
     1921 gggcgtcgcc ggcaacatcg tgctcgcgtg ggtcctgacg atcccgatga gcgcgggctt
     1981 cgcctatctc gcgctgtggg gcctgcgcgg gatcttcggt cccgaggcct gacgacgccg
     2041 cggggccgcg ccggcggccc gcgtcgatcg tgtcgcactc cgtgagtgcc agtgcgccgt
     2101 gtcggatgat gaatcgtcca actcgtctgg cgaaatgggc tgtggtggcc tccagggagg
     2161 gtcccagacg gatttagcaa aatatgccgg cacgccctgc ggacagtttg ccgcccgggt
     2221 gttttactgg tgcggtggaa agttcgttca aggcgcatgg ggctccgttc gcggtcgtcg
     2281 gtttcacgga cgacctgcga gttcgtgcgt ggagcgggag ggcggagcag atttttggcc
     2341 ggacggaggc cgacgcactc gggggcaacg tcgccgagct ggtgccggtc gacggcggcg
     2401 aggcccgctg gcgcgagctg ctgagcgacg acgacgggtc gacggcctgc ctccagcacg
     2461 ccgacgggcg cgtgtgggag tggacgctga cgatggtgcg cgacggcgag gtgcgcggcg
     2521 tggtctgtta cgggcgcgac gtcaccgagc gggtcgcgga gaagaaccag gccgagctcg
     2581 accgcgtgat gctgcatgcg atcctcgaca acttgccggt cgtcgccgtc gtgttggaca
     2641 aggacggcac ctaccagatg atggagggca aggggctgga gggcgtgggg gtcaagccgg
     2701 ggcagatggt cgggctcaac gctcttgagg tgttgaagga caacccggag gtggtcgagt
     2761 acctgcgtcg cggtctcgcc ggcatccagg cgccgccgag cacgctgttc gagtacgggc
     2821 gctacatgga gagctggcac atcccgctgc cgccgggcgg gcggtcgcgg atggtcagca
     2881 tctcgctcga cgtctccgag agcaggttgc gcgagatgga gctgctcgag aagctcgacc
     2941 tgatcgagcg ccagcagcgg gtgatccgcg agctgtcgac gccgatcatc gaggtgtggg
     3001 agggcgtcgt ggccctgccg atcatcggcc tggtcgacag catgcgcacc tcggacatca
     3061 tggacagcct gctgcagacc gtggtccgcc tgcgggcccg ccacgcgatc ctcgacatga
     3121 cgggggtcga cgtcgtcgac accgcgacgg ctaaccacct gatcggcatg atccgcgcgg
     3181 tgcggctgct cggcgcggag ggcatcctga ccggcatcca tcccggcatc gcccagacga
     3241 tcgtcaccct cggggtcgac ctgcgcggga tcacggtgca cgcgacgctg cgtcaggcgc
     3301 tcgtctattg catcggccgc gcgcgcaaga cgaaggcctg acgcgcggac gcgcggcgtg
     3361 acagcgtcac gtcggtcgtc gactcactcg gggtcgcaag ggccgtcgca gaagctctcg
     3421 atcttgcccg tgcg
//
LOCUS       DASWNJ010001978         4602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_140215, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001978 DASWNJ010000000
VERSION     DASWNJ010001978.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140215"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VGB85_32925"
     CDS             <1..457
                     /locus_tag="VGB85_32925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEY0138952.1"
                     /translation="PRFVACTPQGCMRLLETARVDLAGKRALVVGRSTIVGKPMAQLL
                     VHADATVTVAHSRTADLAAEVARADVLVAAIGRAEHIQGAWIKEGAVVIDVGMNRRDD
                     GTLVGDVEYAAAAKRASAITPVPGGVGPMTIACLLSNVVDAAERRAGLR"
     gene            complement(607..1605)
                     /locus_tag="VGB85_32930"
     CDS             complement(607..1605)
                     /locus_tag="VGB85_32930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0138953.1"
                     /translation="MSRGRTPVKDTYSTHDVARICCVTPTTVIRWIEDGLIPAFKTVG
                     GHRRVRREDLERVCGERGIPFTVPTGSEVGRILVIDDEPVVLDLVRDVVKELSHQFDV
                     EVARDAFDAGRSVALFRPQLIFLDLMMPGVDGFEVCTRLKRDPATTHTEIIAITGYYT
                     EANMERILAAGAGACLKKPLDVVDVRRRVLEAFNLREDVRHQPSGPEPAARDREIARV
                     LLVTQNADFRARVKEELGQGSPTVEVMTAQTGADALLVAQSAPPRYVILSMTVPDLKT
                     SEVIQKLAALGSPPQIIAVHDAPNDEIRTTARAAGARMCLPTAAVAGSVRDLLGMT"
     gene            complement(1620..4145)
                     /locus_tag="VGB85_32935"
     CDS             complement(1620..4145)
                     /locus_tag="VGB85_32935"
                     /inference="COORDINATES: protein motif:HMM:NF024818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0138954.1"
                     /translation="MADRTLHQGMPVSPFAAFGEIVDALAVPTAVLNPAGIVVACNQA
                     LLEHLHRPRPEVVGEPLLGWIARASERAAFGRAFMGMRPRLPGHRFSLEISLAPALGR
                     PLPCLVRACKLASDNVLVTCEPEQGERVPETEMGRALTRGLEALDHGLLLLDGQGRIV
                     HANPAARELFSGIGSPRAISDDAPGLPAGGGLLGRSLLELADPEGARTLSQGMSQARA
                     GNWHGELDLRRLDGEIVAVELSLAAGRGEGSPMVALIRDLRERRLREFEARLAAQVDR
                     LLASSLRPQESVAAACTAVMTGLGFARAALLARVGEGWERWQLRADHGPRFGTLDPDL
                     APPATWAAGAPVADVEPNDPAVKPLLGDGDPKCPILRVVLGGPSRVVGHLLLRAAEPA
                     SVDPQAHRLLATLAPQLALGLSNGLLMLETEALAGYQAMVLDQTTVLLDSLDADGRVI
                     TWNRASEQLLGVSAEQARGRRFGVDVARLQGPASWDELWETLHRTGQFVGEMSVLDAN
                     GAAIPLHLEGRLLRDRAGAHPSASAADDPGLVIGAVLVGLDLRHRRALEDQILKAQKL
                     AAVGLLAAGIAHEINNPLSGVVGYSKLLLERQTLDPDVRVKVEKIAASGERCRKIVEG
                     VLLFSRQTEGGKRRKVELRTLIDRVIGIGEYQWRMHNVRVLREGLTGAQVLADADQLE
                     QVLLNLLSNAVDAMPRGGTVRLGLGYEADGRVKLEVSDEGVGIPPEIQGRIFDPFFST
                     KEIGKGTGLGLAISYGILKDHGGDILVRSKPNQGTTFTILLPPEGSPEAIASAATSPS
                     GTSGTSGISGTSTSSTGPASAMATTRATGTPAGPTPPRNKPAT"
     gene            4390..>4602
                     /locus_tag="VGB85_32940"
     CDS             4390..>4602
                     /locus_tag="VGB85_32940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138955.1"
                     /translation="MSITVDPSSPPKPPRPAATVVLLRAAASGGPPAIFMLRRSARSP
                     FMPDALVFPGGAVDPGDGPAGSDEAFV"
BASE COUNT          616 a         1554 c         1790 g          642 t
ORIGIN      
        1 cccgcgcttc gtcgcctgca ccccacaagg ctgcatgcgc ctgctcgaga cggccagggt
       61 cgacctcgcg ggcaagcgcg cgctcgtcgt cgggcgctcg acgatcgtcg gcaaaccgat
      121 ggcccagctg ctggtccacg ccgacgcgac ggtgacggtc gcccactcgc gcaccgccga
      181 cctcgccgcc gaggtcgcgc gcgccgacgt gctcgtcgcg gcgatcggcc gcgccgaaca
      241 catccagggg gcgtggatca aggagggcgc cgtcgtgatc gacgtcggca tgaaccgccg
      301 cgacgacggc acgctcgtcg gcgacgtcga gtacgccgcg gccgcgaagc gagcctccgc
      361 gatcacgccg gtgcccggcg gcgtcggccc gatgacgatc gcgtgcctgc tgagcaacgt
      421 ggtcgacgcg gcggagcgcc gcgccggcct gcgctgaggc tgctgccgct cgaggcagtc
      481 acgggcctta cgcgcccgtc atcgcgcacg tcgggctggg cagcgagacg acgcgtggtc
      541 tcaagccggc gtgctgacgt cgaagccatc gacctcgacg tcccgaagcc catgtccggg
      601 tttagctcag gtcatgccga gcaggtcgcg gacgctgccg gcgaccgcgg cggtcggcag
      661 gcacatgcgg gccccggccg cgcgggcggt ggtgcggatc tcgtcgttgg gggcgtcgtg
      721 cacggcgatg atctgcggcg gggagcccag ggcggcgagc ttttggatca cctcgctggt
      781 cttgaggtcc gggacggtca tcgacaggat cacgtagcgc ggcggggccg actgggcgac
      841 cagcagcgcg tcggcgccgg tctgggcggt catcacctcg acggtcgggc tgccctggcc
      901 gagctcctcc ttcacgcggg cgcggaagtc ggcgttctgc gtgacgagca ggacacgggc
      961 gatctcgcgg tcgcgggcgg ccggctcggg gccgctgggc tggtgacgga cgtcctcgcg
     1021 gaggttgaag gcctcgagca cgcggcggcg cacgtcgacg acgtcgagcg gcttcttgag
     1081 gcacgcaccg gcgccggcgg ccaggatgcg ctccatgttg gcctcggtgt agtagccggt
     1141 gatcgcgatg atctcggtgt gcgtggtcgc cgggtcgcgc ttgaggcggg tgcagacctc
     1201 gaagccgtcg acgccgggca tcatcaggtc gaggaagatc agctgcgggc ggaacagggc
     1261 gacgctgcgg ccggcgtcga aggcgtcgcg ggcgacctcg acgtcgaact ggtgcgagag
     1321 ctccttgacg acgtcgcgca cgaggtcgag cacgaccggc tcgtcgtcga tgacgaggat
     1381 ccggccgacc tcactgccgg tcggcacggt gaacgggatg ccccgctcgc cgcacacgcg
     1441 ctcgagatct tcgcggcgca cgcgacggtg gccgccgacg gtcttgaagg cggggatcag
     1501 cccgtcctcg atccaccgga tcacggtcgt cggggtgacg cagcagatcc gcgccacgtc
     1561 gtgggtggag taggtgtcct tgactggggt acgtccgcgg ctcatgggga tttcttgtct
     1621 caggttgccg gcttattgcg gggaggagtg gggccggccg gggtgccggt ggcgcgggtg
     1681 gtcgccatgg cagaagcggg tccggtggaa gaagtggagg tgccggagat accggatgta
     1741 ccggatgtgc cggaagggga ggtcgcggcc gaggcgatcg cttccgggct gccctcgggc
     1801 gggagcagga tcgtgaaggt cgtaccctgg ttgggcttcg accggaccag gatatcacct
     1861 ccgtggtcct tgaggatgcc gtacgagatc gcgagcccga ggccggtgcc cttgccgatc
     1921 tctttcgtgc tgaagaaggg gtcgaagatg cggccctgga tctccggcgg gatcccgacg
     1981 ccctcgtcgg agacctcgag cttgacccgg ccgtcggcct cgtagccgag gcccaggcgg
     2041 accgtaccgc cgcggggcat cgcgtcgacc gcgttgctga gcaggttcaa gagaacttgt
     2101 tccagctggt cggcgtcggc gagcacctgg gcgccggtga ggccctctcg tagtacgcga
     2161 acgttgtgca tgcgccactg gtactcgccg atgccgatca cgcggtcgat cagggtgcgc
     2221 agctcgacct tgcgacgctt gccgccctcg gtctgacggg agaacagcag cacgccctcg
     2281 acgatcttgc ggcagcgctc gccgctggcc gcgatcttct cgaccttgac gcggacgtcg
     2341 gggtcgaggg tctggcgctc gagcagcagc ttgctgtagc cgacgacgcc gctgagcggg
     2401 ttgttgatct cgtgggcgat gccggcggcc agcaaaccga ccgcggcgag cttctgggcc
     2461 ttgaggatct ggtcctcgag cgcgcgtcga tggcgcaggt cgaggccgac gagcacggcg
     2521 ccgatcacca gaccggggtc gtcggccgcc gaggccgacg ggtgggcgcc ggcccggtcg
     2581 cgcagcagtc gaccctcgag gtgtaggggg atcgccgcgc cgttggcatc gagcacgctc
     2641 atctcgccga cgaactggcc ggtgcggtgc agcgtctccc acagctcgtc ccacgaggcg
     2701 gggccttgca ggcgggcgac gtcgacgccg aaccggcgcc cgcgggcctg ctcggcgctg
     2761 acgcccagca actgctcgct ggcgcggttc caggtgatca cgcgtccgtc ggcgtcgagc
     2821 gagtcgagca gcacggtggt ctggtcgagg accatcgcct ggtagccggc gagcgcctcg
     2881 gtctcgagca tcagcaggcc gttcgacagg ccgagcgcga gctgcggggc gagggtcgcc
     2941 agcaggcggt gggcctgcgg atcgacgctc gccggctccg cggcgcgcaa cagcaggtgg
     3001 ccgacgaccc gcgacgggcc gccgagcacg acccgcagga tcgggcactt ggggtcgccg
     3061 tcgccgagca gcggcttgac ggcggggtcg ttgggctcga catccgcgac gggggcgcca
     3121 gccgcccagg tcgccggcgg ggcgaggtcg gggtcgaggg tgccaaagcg cgggccgtgg
     3181 tcggcgcgca gctgccagcg ctcccagccc tcgccgacgc gcgccagcag ggccgcgcgg
     3241 gcgaagccga ggccggtcat cacggcggtg caggccgcgg cgacgctctc ttgcgggcgc
     3301 aacgaggacg cgagcaggcg gtcgacctgg gccgcgaggc gagcctcgaa ctctcgcagc
     3361 cgtcgctcac gcaggtcgcg gatcagggcg accatcggcg aaccttcacc acgccccgcg
     3421 gcgagcgaga gctcgacggc gacgatctcc ccgtcgaggc ggcgcaggtc gagctcgccg
     3481 tgccagttgc cggcgcgggc ctggctcatg ccctggctga gggtgcgtgc gccctcgggg
     3541 tcggcgagct cgagcaggct gcggccgagc aggccgccac cggcgggcag gcccggggcg
     3601 tcgtcggaga tggcccgcgg cgagccgatc ccggagaaca gctcacgcgc ggccgggttg
     3661 gcgtggacga tgcggccctg gccgtcgagc aacagcaggc cgtggtcgag ggcctcgagg
     3721 ccgcgggtca gcgcgcggcc catctcggtc tcgggcacgc gctcgccctg ttcgggctcg
     3781 caggtgacga ggacgttgtc gctggcgagc ttgcaggcgc ggaccaggca gggcaggggc
     3841 cgaccgagcg ccggggcgag cgagatctcg aggctgaagc ggtggccggg caggcggggc
     3901 cgcatgccca tgaacgcccg gccgaacgcg gcccgctcgc tggcgcgcgc gatccacccg
     3961 agcagcggct cgccgacgac ctcgggacgc ggccggtgca ggtgctcgag cagcgcctgg
     4021 ttgcaggcga cgacgatgcc ggcggggttg agcacggcgg tcgggaccgc gagcgcgtcg
     4081 acgatctcgc cgaaggcggc gaacggcgac accggcatgc cctgatgcag ggtgcgatcg
     4141 gccattgtcc cgctggggac gggaccgccg ctgcgcggag gaaaggatgg cttggaaccc
     4201 tgagccacgg agagggccca cccatagcaa aattaagcgg cccgtgcccg tgttcgccgg
     4261 tggcgatcgc gccgacggcg tttcgcgggc gtccgggggg ctcctgcggg cattcgcgga
     4321 tcgtcgcggg gcgcctcggc gattttttgg tcgagcgacg cgccggaccc gctagtctcg
     4381 gcaccgcgca tgtcgatcac cgtcgatccc tcttcccccc cgaagccgcc gcgcccggcc
     4441 gccaccgtgg tcctgttgcg agcggccgcc agcggcggtc cgccggcgat cttcatgctc
     4501 cgccgcagcg cccggagccc gtttatgccc gacgcgctgg tgttcccggg cggggcggtc
     4561 gaccccgggg acggcccggc cggcagcgac gaggccttcg tc
//
LOCUS       DASWNJ010001979         2318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_140232, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001979 DASWNJ010000000
VERSION     DASWNJ010001979.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140232"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /gene="mazG"
                     /locus_tag="VGB85_32945"
     CDS             complement(<1..786)
                     /gene="mazG"
                     /locus_tag="VGB85_32945"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEY0138956.1"
                     /translation="MTLPRDPGEAKASSDAPSQTQGETGPEAGPLPLQPLHRRLGLRD
                     GIDGLRDLMNRLLAEDGCPWDREQTLDTLRPYLIEEAYEVLEAMDDPESHRRELGDLL
                     LQIVFQSAIREREGAFDLEGVIAAIREKMIRRHPHVFARGEGEAAPTDAAAVSRQWAQ
                     IKEAERSEGSAGAVLRPLAGVPRALPALQRAWRLQDKAASVGFDWPTIDGASAKIREE
                     WEELDQACRSGDQEHVAEEFGDLLFVLVRYGQKLGITAEDALRA"
     gene            822..1970
                     /locus_tag="VGB85_32950"
     CDS             822..1970
                     /locus_tag="VGB85_32950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY0138957.1"
                     /translation="MTIKLSFEDQVPLQLVLGSVGGQAMEELQRSTGVTLHVRGGEVT
                     IDGEDSAAALVERLLRQMVGMARGGTPIHAGDLSRALEVMRREPRIDLRTLFDDAVVG
                     KTGSGRPIAPRSLAQKYYVDCLRRYDLNFGVGPAGTGKTYLAVAMGVRMLMDKRVRRI
                     LLTRPAIEAGENLGFLPGTFEEKISPYMRPLYDALHDMLDPDTIPRLLTAGTIEVAPL
                     AYMRGRTLSETFLILDEAQNTTPEQMKMFLTRIGQGTRAVVTGDPTQNDLPGGRRSGL
                     THALKVLRGVEGIGICPFTAADVMRHQLVQRIIIAYDRDDQRRRTAPNASNGGSRRPE
                     LLPEAAPSSDDAPDREVDDVLPAPVEPDEPVADVIPDVLVPKIEVTGS"
     gene            2117..>2318
                     /locus_tag="VGB85_32955"
     CDS             2117..>2318
                     /locus_tag="VGB85_32955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138958.1"
                     /translation="MRLSTAWLAPITVLALTACGSESAGGTDDESTAASSGSSGAPAT
                     GTGTTGEPPTTDPTTGAPATTSG"
BASE COUNT          337 a          887 c          772 g          322 t
ORIGIN      
        1 ggcccgcagc gcgtcctcgg cggtgatgcc gagcttttgc ccgtagcgga ccagcacgaa
       61 cagcaggtcg ccgaattcct ccgcgacgtg ctcctggtcg ccggagcggc aggcctggtc
      121 gagctcctcc cactcctcgc ggatcttcgc cgacgcgccg tcgatggtcg gccagtcgaa
      181 gccgacgctc gccgccttgt cctgcagccg ccacgcgcgt tgcagggccg gcagcgcccg
      241 ggggacgccg gcgagcggtc gcagcaccgc gccggccgac ccctcgctgc gctccgcctc
      301 cttgatctgc gcccattgcc tgctgaccgc ggccgcgtcc gtgggcgctg cttcgccctc
      361 gccgcgggca aacacgtgcg ggtggcggcg gatcatcttc tcgcggatcg ccgcgatcac
      421 gccctcgaga tcgaaggcgc cctcacgctc gcggatcgcc gactggaaca cgatctgaag
      481 cagcaggtcg ccgagctcgc ggcggtgcga ctccgggtcg tccatcgcct cgagcacctc
      541 gtaggcctcc tcgatcaggt acgggcgcag cgtgtcgagg gtctgctcgc ggtcccacgg
      601 gcagccgtcc tcggcgagca ggcggttcat cagatcgcgc aggccgtcga tgccgtcgcg
      661 caggccgagc cgcctgtgca gtggttgcag cggcaacggc cccgcctcgg gcccggtctc
      721 gccctgcgtc tgcgagggcg cgtcactgct cgcctttgcc tctccggggt cgcgtggtaa
      781 ggtcacggct gcgccatacc acaggggcta cacacccacc cgtgaccatc aaactaagct
      841 tcgaagatca ggtgccgctg caactcgtcc tgggttcggt cggcgggcaa gcgatggaag
      901 agttgcagcg gtcgaccggc gtcacgctgc acgtacgggg cggggaggtg acgatcgacg
      961 gcgaggactc cgccgccgcg ctcgtcgaac ggctcctgcg ccagatggtc ggcatggccc
     1021 gcggcggcac gccgatccac gccggcgacc tctcgcgggc gctggaggtc atgcggcgcg
     1081 agccgcggat agacctccgg accctgttcg acgacgcggt cgtcggcaag accggcagcg
     1141 gccgcccgat cgccccgcgc agcctggcgc agaagtacta cgtcgattgc ctgcgccgct
     1201 acgacctcaa cttcggggtc ggcccggcgg gcaccggcaa gacctacctc gccgtggcga
     1261 tgggcgtgcg catgctgatg gacaagcgcg tgcgccggat cctgctgacg cgcccggcga
     1321 tcgaggccgg tgagaacctc ggctttttgc ccggcacctt cgaggagaag atcagcccgt
     1381 acatgcgccc gctgtacgac gcgctgcacg acatgctcga ccccgacacc atcccgcgcc
     1441 tgctcacggc cgggaccatc gaggtcgcgc cgctggccta catgcgcggg cggacgctca
     1501 gcgagacctt tttgatcctc gacgaggcgc agaacaccac ccccgagcag atgaagatgt
     1561 tcctgacccg cattggtcag ggcacccggg ccgtggtcac cggcgatccg acgcagaacg
     1621 acctgcccgg cgggcgccgc tccggtttga cgcacgcgct caaggtcctg cgcggcgtcg
     1681 agggcatcgg catctgcccg ttcaccgccg cggacgtcat gcgccaccag ctggtccagc
     1741 gcatcatcat cgcctacgac cgcgacgacc agcgccgccg caccgccccg aacgcgtcca
     1801 acggcgggtc ccgccgcccg gagctgctgc ccgaggccgc cccgagcagc gacgacgccc
     1861 cggaccgcga ggtggacgac gtcctccccg cgcccgtcga gcctgacgag cccgtcgccg
     1921 acgtcatccc cgacgtgctc gtgcccaaga tcgaagtcac cggcagctga aggctcgctc
     1981 gaccccgccg accaccccgc cgaccacccc gccgacctgg gttcaccaga cccgggcgcg
     2041 ggtcacgtga tcgatcccgg tagtcgcggt gcatttcggt tgcatgcaca tcggcccgcg
     2101 aaatagggtt gcgatgatgc gtctctcgac cgcctggctc gccccgatca ccgtgctcgc
     2161 cctgaccgcc tgtggctccg agagtgcggg gggcacggac gacgagtcga ccgcggcgag
     2221 cagcggctcg agcggcgccc cagccactgg caccgggacc accggcgagc ccccgacgac
     2281 cgacccgacc accggcgccc ccgccaccac cagcggcg
//
LOCUS       DASWNJ010001980         3806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_175332, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001980 DASWNJ010000000
VERSION     DASWNJ010001980.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175332"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VGB85_32960"
     CDS             complement(<1..455)
                     /locus_tag="VGB85_32960"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEY0138959.1"
                     /translation="MSRLSLFLSVVCLSLAAPACNNKPAEPAKAEAKDVNQIPNADTK
                     AGAAELGALPDPVALVNGKPISLAEFKTIYDLKVAKYAERGREIPPSADRRYRKSITE
                     RLVYQEVLRQEAEAKGVKYNEAELAERETQQKQGIKDWEKHLLRRGETE"
     gene            651..2051
                     /gene="miaB"
                     /locus_tag="VGB85_32965"
     CDS             651..2051
                     /gene="miaB"
                     /locus_tag="VGB85_32965"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEY0138960.1"
                     /translation="MSSLVQIRSPGSATRISADAADPRSADGAPGTISAGARVYLETL
                     GCQMNEADSAIIMGQLRARGYTRVEDPALADVILLNTCAVREKAEDRVYGRTSQLLRH
                     RKDNPDLVFGITGCMAEHLRDKVSQQAPYIGLVAGPDSYRRIGALVDRARTGERVIDV
                     ALDKSETYEGLDGVPDDDGVSGQISIQRGCDKFCTFCVVPYTRGRERGVAPREVLRAA
                     RSLVERGYKEIVLLGQTVNSYAWEDVSFADLLRALAGVDGLERIRFTSPYPVDFSDEL
                     IAMMASEPKICPYMHLPAQSGSDAMLTAMKRGYTREQFLTLVKKLRAAMPDLAISTDL
                     MVGFCGETEADHQETLSLMREVRFDSAFMFRYSDRGITYAARKLQDDVPSDVKGRRLQ
                     EVIDLQEQHTRAAHAARVGGRERVLVSGMSKRGDRTLGRTPSFYTVLLPLGLAAPGET
                     VEVEITGTTGHSLLAG"
     gene            complement(2054..2674)
                     /locus_tag="VGB85_32970"
     CDS             complement(2054..2674)
                     /locus_tag="VGB85_32970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138961.1"
                     /translation="MRRLACVLVFCACQAPGGGTDGGLVVISDTAQMGPPPACNAETP
                     TTGEEGTGDDGTGGSTGGSTGEAVERDAVDLSRRPGASRAQDPGTAFCGGFDTHAACD
                     GTLRMDGDAVVGECRWMSVVPVIPGTCEATQIYSTCVHVPHGAGGCEAAMSCGQVGLG
                     VYGRTGCDGNVEVIVVPPGQAFCHAPTDWPLCWPDDTAPECTCICS"
     gene            3045..3410
                     /locus_tag="VGB85_32975"
     CDS             3045..3410
                     /locus_tag="VGB85_32975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138962.1"
                     /translation="MLLQIRPSQVQALTELAQRRFVADAELHVRGLELPRWREVGAPV
                     LRAWVRRRIDVGVALGLVEELALLRHLEVAARHDERFADSEDVLGLLHSLPDLQRWPE
                     TAELIDLLHAAYVAEATPR"
     gene            3407..>3806
                     /locus_tag="VGB85_32980"
     CDS             3407..>3806
                     /locus_tag="VGB85_32980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138963.1"
                     /translation="MKGKQPAAVRSKAGQPAGKPCATCQAKQKAKPTPPCDFDKIEVK
                     CKHCGKPDKRDIKSVIFALNAATGARTNNLKRSPNPRRALLTRRFEVAGDDDITVTLA
                     GGPGYHGGQHPTMMLTGPDGVAPVVVRGRTQ"
BASE COUNT          552 a         1443 c         1269 g          542 t
ORIGIN      
        1 ttctcggtct cgccgcgacg caggaggtgc ttctcccagt ccttgatgcc ctgcttctgc
       61 tgcgtctcgc gctcggccag ctcggcctcg ttgtacttga cgcccttggc ctcggcctcc
      121 tgacgcagca cctcctgata cacgaggcgc tcggtgatcg acttgcggta gcggcggtcc
      181 gccgacggcg ggatctcgcg gccacgctcg gcgtacttgg cgaccttgag gtcatagatc
      241 gtcttgaact cggcgaggct gatcggcttg ccgttgacca gcgcgaccgg gtcgggcagc
      301 gcgccgagct cggcggcgcc ggccttggta tcggcgttcg ggatctggtt gacatccttc
      361 gcctcggcct tggccggctc ggccggcttg ttattgcagg cgggcgccgc gagggacagg
      421 cagacgacac tcaagaacaa tgaaaggcgg gacatggatt ctcctgaaac gggcgcgtgg
      481 cccggccgac cgtgccgccg gcagcgcggg atcctacgcc cgatgccgcg ccgttccaag
      541 gctttgcatc gacctcgaca cacgcgtgag ctaccgcccg agggcgtgcc cctgcgcccg
      601 cacccgccgc aagcgccccg ccggccgcga tcgcgtagga tcccgcgccc atgtccagcc
      661 tcgttcagat ccgttcaccg ggcagcgcga cccggatctc cgccgacgcg gccgatccgc
      721 gatccgcgga cggtgctccc gggacgatct cggccggcgc gcgtgtctac ctcgagaccc
      781 tcggctgtca gatgaacgag gccgactcgg cgatcatcat gggccagctg cgcgcccgcg
      841 ggtacacgcg cgtcgaggac ccggccctcg ccgacgtcat cctgctcaac acctgcgccg
      901 tccgggagaa ggccgaggac cgcgtctacg ggcgcaccag ccagctcctg cgccaccgca
      961 aggacaaccc ggacctcgtg ttcggcatca ccggctgcat ggcggagcac ctgcgcgaca
     1021 aggtctcgca gcaggccccg tacatcggcc tcgtcgccgg ccccgacagc taccgccgga
     1081 tcggcgcgct cgtcgaccgc gcccgcaccg gcgagcgcgt gatcgacgtc gccctcgaca
     1141 agtccgagac ctacgagggc ctcgacggcg tgcccgacga cgacggcgtc agcggccaga
     1201 tctcgatcca gcgcggctgc gacaagttct gcacgttctg cgtcgtgccc tacacccgcg
     1261 gccgcgagcg cggcgtggcc ccgcgcgagg tgctacgcgc cgcgcggagc ctcgtcgagc
     1321 gcggctacaa ggagatcgtg ctgctgggtc agaccgtcaa ctcctacgcc tgggaggacg
     1381 tgagcttcgc cgacctgctg cgcgcgctcg ccggggtgga cggcctcgag cggatccgct
     1441 tcaccagccc ctaccccgtc gacttctccg acgagctgat cgccatgatg gcgagcgagc
     1501 ccaagatctg cccgtacatg cacctgccgg cgcagtcggg gtccgacgcg atgttgaccg
     1561 cgatgaagcg cggctacacg cgcgagcagt tcctgacgct ggtcaagaag ctgcgcgccg
     1621 cgatgcccga cctcgcgatc tcgaccgacc tgatggtcgg cttttgcggc gagaccgagg
     1681 ccgaccatca ggagaccctg tcgctgatgc gcgaggtgcg gttcgacagc gcgttcatgt
     1741 tccgctacag cgaccgcggc atcacctacg ccgcgcgcaa gctgcaagac gacgtgccct
     1801 cggacgtcaa gggccggcgc ctgcaagagg tgatcgacct ccaggagcag cacacccgcg
     1861 ccgcccatgc ggcccgcgtc ggcggccgcg agcgcgtgct cgtgtccggc atgtcgaagc
     1921 gaggcgaccg caccctcggc cgcaccccca gcttctacac cgtgctgctc ccgctcggcc
     1981 tcgccgcccc cggcgagacc gtcgaggtcg agatcaccgg cacgaccggc cactcgctgc
     2041 tcgccggctg acctcagcta cagatgcatg tgcactcggg cgccgtgtcg tcgggccagc
     2101 acagcggcca gtcggtcggc gcgtggcaaa atgcctgacc cggcggcacg acgatgacct
     2161 cgacgttgcc gtcgcagccg gtgcgcccgt acacgccgag cccgacctgc ccgcacgaca
     2221 tcgccgcctc gcagccgccc gcgccgtgcg gcacgtgcac gcacgtgctg tagatctggg
     2281 tcgcctcgca ggtgcccggg atcaccggca ccaccgacat ccagcggcac tcgccgacca
     2341 ccgcgtcacc gtccatgcgc agcgtgccgt cgcacgccgc gtgagtgtcg aagccgccgc
     2401 agaacgcggt gccggggtcc tgcgcgcgcg aagcacctgg ccttcgggac aggtcgaccg
     2461 cgtcgcgctc gacggcctca ccggtgctcc cgccagtgct cccgccggta ccgtcgtcgc
     2521 ccgtgccttc ctccccggtg gtcggcgtct ccgcgttgca ggccggcggc gggcccatct
     2581 gcgcggtgtc cgagatgacg acgaggccac catcggtgcc ccctccgggc gcctgacagg
     2641 cgcagaacac gagcacgcac gcgaggcgtc gcatcggcgc acgctaccgc ctctccagcg
     2701 cgcacgggtg cgacttcgct cacgaatatt cgcccccgcg acggcccgca gcctccgggc
     2761 cgggatccat gggccgggcg gcccgaccgg cgcacagccg ctccgacctg cccttcacga
     2821 catgtcccaa aggccatccg ggccagcacg acaagatcgt ccgcgaccga cgagcatgcc
     2881 cgcgatcatg atcgctaccc ccgggcgttt tggcgcctgc ccgcgagccc cccgcgacgc
     2941 cgcgcgtgtt cgctgttcct cgcgcagatc gccgctgtag gcggcccgcg ggccgttgtt
     3001 tatggtggcg atgggccgtc gggtaggact tcccgacctt catcatgttg ttgcagatcc
     3061 gtccgtctca ggtccaggcc ctcaccgagc tggcgcagcg acgcttcgtc gccgacgccg
     3121 agctgcacgt ccgtgggctc gagctgccgc gctggcgcga ggtcggggcg ccggtcctgc
     3181 gcgcatgggt gcggcggcgc atcgacgtcg gcgtcgccct cggcctggtc gaggagctgg
     3241 cgctgctgcg gcacctcgag gtcgccgcgc gtcacgacga gcgcttcgcc gactcggagg
     3301 acgtgctcgg gctcctgcac agcctcccgg acctgcagcg ctggccggag accgccgagc
     3361 tgatcgacct gctccacgcc gcgtacgtcg ccgaggcgac ccctcggtga agggcaagca
     3421 accggccgcg gtgcgcagca aggcggggca gcccgcgggc aagccgtgcg cgacctgtca
     3481 ggccaagcag aaggccaagc cgaccccgcc ctgcgacttc gacaagatcg aggtgaagtg
     3541 caagcactgc ggcaagccgg acaagcgcga catcaagagc gtgatcttcg cgctcaacgc
     3601 tgccaccggc gctcgcacca acaacctcaa gcgatccccc aacccccggc gggcgctgct
     3661 cacccggcgc ttcgaggtcg ccggagacga cgacatcacg gtcaccctcg cgggcggccc
     3721 ggggtaccac ggcgggcagc acccgacgat gatgctgacg ggccccgacg gggtcgcgcc
     3781 ggtggtcgtg cgcgggcgga cgcagc
//
LOCUS       DASWNJ010001981         5329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_529545, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001981 DASWNJ010000000
VERSION     DASWNJ010001981.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5329
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529545"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VGB85_32985"
     CDS             <1..582
                     /locus_tag="VGB85_32985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138964.1"
                     /translation="LLVRVRMAQHDDEAAETLVRNGIYTVGAGSSGRLFLAWARLELR
                     QHKRKQAALHARAALHRMQAEGRPTVELLATAEMAVNLFLRTEQIKLACGVTRELVQQ
                     QPDSAEAWRLEARAQLDADENGKARRAIARAIELAPEAPQGHEIRALIALRSGDRKTG
                     VAALQQALTLARDDATRARVTEALRRIPGASPR"
     gene            complement(631..2808)
                     /locus_tag="VGB85_32990"
     CDS             complement(631..2808)
                     /locus_tag="VGB85_32990"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY0138965.1"
                     /translation="MADPVTVFMLVVAGLLVVGTVGEQVFARTGVPSVIWLVALGVLV
                     RIADIVPAPVIHTLAPFFAALALVIVLFDAGTNLTGGTGEAALAPATRTRAQLLAFVG
                     FAVTVVLVAVFSHGLAALGILPSWGWANALMLGSLIACGASEVALPSLRGLPGSAEAT
                     ALLRRESAITKALAVAGTVICLDVLSPRIGEGGAALAMIAGFGFALAFGSVAGMVWVV
                     ALQKSAGALHDGSDSDARNYGFTLAAMLVLHVLSEAAGGSGPLSVLVFGAVLGNAGSV
                     ARMLGRTGEAGESMAGTAVQAALGGHARTISFVRTLLFAMVGLTLAPPWGPLVMGVAL
                     ALFVLVIRLAVGHFTLARLTHHARSIVGACAPRGMATVALATLALAHEVPGAEAMVTL
                     VFSAVTTSVLLFTLGLHGLRGPEPARVEPVVRAPVEVSPEVVEQVREVVVQVVPPVEV
                     IEPVPAVVELVAAASGSGSQAASSGPDLALAAETSRPAGPVPTAQSMQAELSPRPGSE
                     VPARASRPLQSARIAGAVPVPRSADARISGPALAAARISGPLLAARSSERISGPVLAA
                     RSSERISGPLVAARSSERSSGPLLAAPPEVIKIVGPEVADEPDARAPTLPREMSGPQV
                     AADAGRPKVEAPRLPEPEGYLPTLPNIEGAPPVAEDPLAGAMARALARSPAEVTTARR
                     RPTGDYLVPLPTTPFPDTSGEDALSLLAGLGMPENGPKPPRSP"
     gene            complement(2857..3426)
                     /locus_tag="VGB85_32995"
     CDS             complement(2857..3426)
                     /locus_tag="VGB85_32995"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY0138966.1"
                     /translation="MEELDERNERDAQDAGAAEPTTIASGRFLRLVRRGTWEFVERVG
                     ASGAVCIIAVTDDRKLVLIEQPRPAMNGYVIELPAGLVGDGEDAGELGSAAANRELVE
                     ETGFEAAHIEEVATGPVTPGLSNECVALFLATGLRRVGPGGGVDGEQITVHEVPLLEV
                     EAFLREQAARGRTIDLKVYSALHFARRVV"
     gene            3475..4605
                     /locus_tag="VGB85_33000"
     CDS             3475..4605
                     /locus_tag="VGB85_33000"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing transporter"
                     /protein_id="HEY0138967.1"
                     /translation="MQRVLSLDALRGVAVIFMMEQHLGVWLWEQEQPGRGAIAQAPGY
                     MAINGLGGLAAPTFITLAGVGCSLFVATKLRPGPAGEPPRPGAPDGSLDIILVRRGLT
                     LLGFGYLLNFLTPSWFSAGSWFVLHMMGFAIATAPLWRRLPGPALLAAEGVIFALAVA
                     VQSWLATPRLLDNARMRDLELPGGALRLALAEGQFPILPWLALFLVGLVIGRWLLAEQ
                     HRRVGALCRWCLLAGVALVALDTAAREVPAFAVLRQAPWWRVVRVTTSFFPAGPGIVL
                     LLQAGVLLMLTVALHIERSRELDPRGWLVCLGRSSLTLLLVHVVLFREISRPLGAWQA
                     FDRYTSLAIIIAWIGLCVLLCRAWQRIGYRYGAEWLLRRLGG"
     gene            4699..>5329
                     /locus_tag="VGB85_33005"
     CDS             4699..>5329
                     /locus_tag="VGB85_33005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138968.1"
                     /translation="MPDFEAYNDKLAASSGPHEPVSADAHPARASGELVADVAGSADL
                     ADLADIRGSGSFAPFGPADLAAQLVAQADVRGSGGVASFIPAPHEPVRHDAGFASRGS
                     GDFSAHNPDLASTDLAADALRFLGRVFPAVVVPASLFVVVLATQPVVTGLLCLAFLGL
                     NFAASLLAARNARLAEGLRFLGFTAVLLALPSYAGPTAPSWLLGLAVACT"
BASE COUNT          702 a         2204 c         1712 g          711 t
ORIGIN      
        1 ttgctcgtcc gcgtgcgcat ggcccagcac gacgacgagg ccgccgagac cctggtgcgc
       61 aacggcatct acaccgtcgg cgccggcagc agcggccgcc tgttcctcgc ctgggcccgg
      121 ctcgagctgc gccagcacaa gcgcaagcag gccgcgctgc acgcccgcgc cgcgctgcat
      181 cgcatgcagg ccgagggccg gccgaccgtc gagctgctgg cgaccgccga gatggccgtc
      241 aacttgttcc tgcgcaccga gcagatcaag ctcgcctgcg gcgtcacgcg cgagctcgtg
      301 caacagcagc ccgacagcgc cgaggcctgg cggctcgagg ccagggctca gctcgacgcc
      361 gacgagaacg gcaaggcccg ccgcgcgatc gcccgcgcga tcgagctcgc cccggaggcc
      421 ccccagggcc acgagatccg cgccctgatc gccctgcgca gcggcgaccg caagaccggc
      481 gtcgccgccc tgcagcaggc cctcaccctc gcccgcgacg acgccacccg cgcccgcgtc
      541 accgaagccc tgcgacggat ccccggcgct tcgccgcgat gaatatcatc agagtcatcg
      601 tcgcacgcaa agtgtcgaca atgaccggcg ctacgggctc ctcggcggct ttggcccatt
      661 ctcaggcata ccgagcccgg cgagcagcga cagcgcgtcc tccccgctgg tatccgggaa
      721 cggcgtggtc ggcagcggca ccagataatc gccggtcggc cggcgtcgcg ccgtcgtgac
      781 ctcggcgggg ctcctggcca gcgcgcgggc catcgcgccc gccagcggat cctcggcgac
      841 cggcggtgcg ccctcgatgt tgggcaacgt cggcaggtag ccctccggct cgggcaagcg
      901 cggcgcctcg accttcggcc ggcccgcgtc ggccgcgacc tgcggcccac tcatctcgcg
      961 cggcagcgtc ggcgcccgcg cgtccggctc gtcggcgacc tcgggcccga cgatcttgat
     1021 cacttcgggc ggcgctgcca acagcggccc ggagctccgc tccgacgagc gcgctgccac
     1081 cagcgggccc gagatccgct ccgacgagcg cgccgccagc accggccccg agatccgctc
     1141 cgacgagcgc gccgccagca agggcccgga gatccgcgcc gctgccagcg cgggcccgga
     1201 gatcctcgcg tcggcgctgc gcggcaccgg gaccgccccg gcgatgcggg cagattgcag
     1261 cggccgggag gcccgcgccg ggacctccga acctggcctc ggggacagct ccgcctgcat
     1321 gctctgcgcc gtcggtaccg gccccgcggg ccgcgacgtc tccgccgcca gcgccaggtc
     1381 cggaccggag gacgccgcct gcgaccccga ccccgaagcc gccgccacca gctcgaccac
     1441 cgccggcacc ggctcgatca cctcgaccgg cggcaccacc tgcaccacca cctcacggac
     1501 ctgttcaacg acctccgggc tgacctccac tggcgcgcgg accaccggct cgacgcgcgc
     1561 cggctccggt ccgcgcagcc cgtgcagccc gagcgtgaac agcagcacgc tcgtcgtgac
     1621 cgccgagaac accagcgtga ccatcgcctc cgcgccgggc acctcgtggg ccagggcgag
     1681 ggtcgccagc gcgacggtcg ccatgccccg gggagcgcag gcgccgacga tgctgcgcgc
     1741 gtggtgtgtg agcctggcga gcgtgaagtg gccgaccgcg agccggatca ccagcacgaa
     1801 caatgccagc gccacgccca tcaccagcgg cccccacggg ggtgccagcg tcagcccgac
     1861 cattgcgaac agcagcgtgc gcacgaacga gatcgtccgt gcgtggccac cgagcgccgc
     1921 ctgcacggcc gtgccggcca tcgactcgcc cgcctcgccg gtgcgcccga gcatccgggc
     1981 gacgctcccc gcgttgccga gcaccgcccc gaacaccagc accgacagcg gccccgagcc
     2041 gcccgccgcc tccgagagca cgtgcagcac cagcatcgct gcgagcgtga atccgtagtt
     2101 acgcgcgtcg gagtccgagc cgtcgtgcag cgcccccgcc gacttttgca gcgcgaccac
     2161 ccacaccatc ccggcgacgc tgccgaacgc gagcgcgaag ccgaagcccg cgatcatcgc
     2221 cagcgccgcc ccgccctcac cgatccgcgg gctgagcaca tcgaggcaga tcaccgtgcc
     2281 cgcgaccgcg agcgccttcg tgatcgccga ctcgcgccgc agcagcgccg tcgcctccgc
     2341 cgagcccggc agcccgcgca gcgacggcaa cgcgacctcc gacgccccgc acgcgatcag
     2401 cgagccgagc atcagcgcgt tcgcccagcc ccagctcggc aagatcccga gcgccgcgag
     2461 cccgtgacta aatactgcca ccagcacgac cgtcaccgcg aagccgacga acgccagcag
     2521 ctgcgcgcgc gtgcgggtcg ctggcgcgag cgcggcctca cctgtccctc cggtcaggtt
     2581 ggtaccggcg tcgaacagga cgatcaccag cgcgagcgcg gcgaagaacg gcgcgagcgt
     2641 gtggatcacc ggcgccggca cgatgtcggc gatgcggacg agcaccccga gcgcgaccag
     2701 ccagatcacc gagggcacgc cggtgcgggc gaacacctgt tcgccgaccg tgccgaccac
     2761 caggaggccg gcgaccacca gcatgaagac ggtgacggga tcggccactg tcccgctctt
     2821 atgccacaag cgcgaccgga acgatcacac cacggattac accacgcgcc gcgcgaagtg
     2881 cagcgcggaa tacaccttga ggtcgatcgt ccgcccgcgc gccgcctgct cgcgcaggaa
     2941 tgcctcgacc tcgagcaatg gcacctcgtg caccgtgatc tgctcgccat cgacgccgcc
     3001 gccgggcccg acccgccgca ggccggtcgc caggaacagc gcgacgcact cgttcgacag
     3061 ccccggcgtc accggcccgg tcgcgacctc ctcgatgtgc gccgcctcga agcccgtctc
     3121 ctcgaccagc tcgcggttcg ccgcggcgct gccgagctcg cccgcgtcct cgccgtcgcc
     3181 caccaacccg gccggcagct cgatcacgta gccattcatc gccgggcgcg gctgctcgat
     3241 cagcaccagc ttgcggtcgt cggtcaccgc gatgatgcac accgcgcccg atgcgcccac
     3301 gcgctcgacg aattcccacg tcccgcgacg gaccagtcgg aggaaccgcc ccgaggcgat
     3361 cgtcgtcggc tcggccgccc ctgcgtcctg cgcgtctcgc tcgttgcgct cgtctagctc
     3421 ttccatggcc gcgagtgtag cgcgagcgcg cgccgtcgcg gtagcctctc gcgggtgcaa
     3481 cgtgtgctca gcctcgacgc cctgcgcggc gtcgccgtca tcttcatgat ggagcagcac
     3541 ctcggtgtgt ggctgtggga acaggagcag cccgggcgcg gcgcgatcgc ccaggcgccc
     3601 ggctacatgg cgatcaacgg cctcggtggc ctcgcggccc cgaccttcat caccctcgcc
     3661 ggcgtcggct gctccctgtt cgtggccacg aaacttcgtc ccgggcccgc tggcgagccg
     3721 ccccgccccg gcgcgcccga cgggtccctc gacatcatcc tcgtccgccg cggcctgacg
     3781 ctgctcggct tcggctacct gctcaacttc ctgaccccga gctggttctc cgccggctca
     3841 tggttcgtgc tgcacatgat gggctttgcg atcgccacgg ccccgctgtg gcgccgcttg
     3901 cccggccccg cgctgctcgc cgccgaaggg gtgatcttcg ccctcgccgt cgccgtgcag
     3961 tcgtggctcg ccaccccgcg gctcctcgac aacgcgcgga tgcgcgacct cgagctgccc
     4021 ggcggcgccc tgcgactcgc cctcgccgag ggtcagttcc cgatcctccc ctggctcgcc
     4081 ctgttcctcg tcggcctcgt catcggccgc tggctgctcg ccgaacagca ccggcgcgtc
     4141 ggcgccctgt gtcgctggtg tctgctcgcc ggcgtcgcgc tcgtcgccct cgacaccgcc
     4201 gccagggagg tgcccgcgtt cgcggtgctg cgccaggcgc cgtggtggcg ggtcgtgcgc
     4261 gtgacaacga gcttcttccc cgcgggtccg gggatcgtcc tgctgctgca agcgggcgtc
     4321 ctgctgatgc tgaccgtcgc gctgcatatc gagcgctccc gcgagctcga cccgcgcggc
     4381 tggctggtct gcctcggccg gtcctcgctc accctgctgc tcgtccacgt cgtgctcttc
     4441 cgtgagatct cccgccctct cggcgcgtgg caggccttcg accgctacac ctcgctcgcg
     4501 atcatcatcg cgtggatcgg cctgtgcgtg ctcctgtgcc gcgcctggca gcggatcggc
     4561 taccgctacg gggccgagtg gctcctgcgt cggctcggcg gctagtcacc acgcaacccc
     4621 gctcgccaaa acgcgcgcac cattcacgac gcagcgccgc accgccgaca agtagtatgt
     4681 tccgcagggc gcctggccat gccggatttc gaggcctaca acgacaagct cgccgcgtcc
     4741 tccggaccgc acgaacccgt gtccgcggat gcccaccccg cgcgagcctc cggcgagctc
     4801 gtcgccgatg tcgccggttc cgccgacctc gccgacctcg ccgacatccg cggctccggc
     4861 agcttcgcac cgtttggacc cgccgacctc gccgcccagc tcgtcgccca ggccgacgtc
     4921 cgcggctccg gcggcgtcgc ctccttcatc cccgccccgc acgagcccgt ccgccacgac
     4981 gccggcttcg cctcgcgcgg ctccggcgac ttctccgcgc acaaccccga cctcgcctcg
     5041 accgacctcg ccgccgacgc cctgcgcttc ctcggtcgcg tgttcccggc ggtcgtcgtg
     5101 cccgcgtcgc tgttcgtcgt cgtgctcgcc acgcagccgg tcgtcaccgg tctgctgtgc
     5161 ctcgccttcc tcgggctcaa cttcgccgcc tcgctgctcg ccgcgcgcaa cgcccggctc
     5221 gccgagggtc tgcgcttcct cggcttcacc gccgtgctgc tcgccttgcc gagctacgcc
     5281 ggcccgaccg ccccgagctg gctgctcggc ctcgccgtcg cctgcaccg
//
LOCUS       DASWNJ010001982         1955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_529567, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001982 DASWNJ010000000
VERSION     DASWNJ010001982.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529567"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..1029
                     /locus_tag="VGB85_33010"
     CDS             190..1029
                     /locus_tag="VGB85_33010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138969.1"
                     /translation="MDPATSSAPQSQDIDWARLDPRTVVIATPQRAWVADTAPADRQP
                     ARASIATTRKLLDGAIALAQRQTPAAEPPPPLSPLRWLWRLAGYYQLTRATPRLMAEA
                     ADRFLAAGEPELAAWAAEKAHEEQGHDRLALRDIAALGYDAERVVIALVPEPAVALVG
                     RFTAYVRRTDPLACVGYAHTLERLALEVRRPEIDAVQALLPAGVDATRCMRVHSGIGS
                     DAAHVDDNVALIAALPPHRRAVVIAAVHETATLCMRPPPGGHVTDDAIARAISVTSSH
                     QLH"
     gene            1073..1237
                     /locus_tag="VGB85_33015"
     CDS             1073..1237
                     /locus_tag="VGB85_33015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138970.1"
                     /translation="MNKQDPNTTTEPTTTTEPTATEATESFTVVAHDSDDLQVQPFER
                     GPLTDLNFGF"
     gene            1398..>1955
                     /locus_tag="VGB85_33020"
     CDS             1398..>1955
                     /locus_tag="VGB85_33020"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEY0138971.1"
                     /translation="MSTSHCDVVVLGGGPAGSTFASIVKKYRPQASVVVLEKARFPRW
                     HIGESTIPAANGVFKDLGVYDTLLRSDFVKKMGVTFVWGQDRQPWSADYLTLRDTTGK
                     AVPGSVIDVTGQDFSALLGPGATHDTPYSAFNVRRAEFDKLLLDQSRHFGVDVREGTA
                     ARQVVRGAAGQAHTIHWQDDQGHSGA"
BASE COUNT          317 a          793 c          583 g          262 t
ORIGIN      
        1 gacctcgccg tgacccacga gggttgacgc gtccccgcgg agcgcccccg cgcaccgccg
       61 gcccgcgagg ctcccggccg ccgcgccgca aaatctcacg cttcgcgcgg ccatgcccgc
      121 gacgcaccgg cctcgcaaaa aaaatgcttc gagcgcgatc gtttcggggt cggctgccac
      181 aaaaggggca tggacccggc aacgagcagc gcccctcaat cacaggacat cgactgggcc
      241 cgcctcgacc cgcgcaccgt ggtgatcgcc acgccgcagc gggcctgggt cgccgacacg
      301 gcgcccgcag atcgccagcc ggcgcgggcc agcatcgcca ccacgcgcaa gctgctcgac
      361 ggggcgatcg cgctggccca gcgtcagacc cccgccgccg agcccccgcc gccgctcagc
      421 ccgctgcgct ggctatggcg cctcgccggg tactatcagc tgacccgcgc caccccgcga
      481 ttgatggccg aggccgccga ccgcttcctc gccgccggtg agcctgagct cgccgcgtgg
      541 gccgccgaga aggcccacga ggagcagggc cacgaccgcc tcgcgctgcg tgacatcgcc
      601 gcgctcggct acgacgcgga gcgcgtcgtc atcgccctcg tacccgagcc cgccgtcgcg
      661 ctcgtcggca ggttcaccgc ctacgtgcgc cgcacggacc cgctcgcctg cgtcggttat
      721 gcacacacgc tcgagcgcct cgcgctcgag gtccggcgcc ccgagatcga cgccgtgcag
      781 gccctgttgc cggccggcgt cgacgcgacc cgctgcatgc gcgtgcatag cgggatcggc
      841 agcgacgccg cgcacgtcga cgacaacgtc gccttgatcg cagcactccc gccgcaccgt
      901 cgcgccgtcg tcatcgcggc cgtccacgag accgccaccc tttgcatgcg acccccgccc
      961 gggggccacg tcaccgacga cgcgatcgca cgggcgatct ccgtcacttc ctctcaccaa
     1021 ctgcactgaa aagccttcca ctcgccacat cgtcagacaa ggaaaataca ccatgaacaa
     1081 gcaagacccg aacaccacca ccgagcccac caccaccacc gagccgaccg cgaccgaggc
     1141 caccgagagc ttcaccgtcg tggcgcacga cagcgacgac ctccaggtgc agcccttcga
     1201 gcgcggcccg ctgaccgacc tcaacttcgg cttctgagac cctctcaccc gcccgggggc
     1261 gccccgcgcc cccgacccct tttatcaaaa gcgcccgcat caccgcgaac gcgcgcaggt
     1321 gcaccgaggg ccgctctgct cgccagcaat agcccctcct ctctctcgca gcagttcatt
     1381 tcaggagctt ctcgcaaatg tccacctccc attgtgacgt cgtggtgctc ggcggcggtc
     1441 cggccggcag cacctttgcc agcatcgtca agaagtaccg cccgcaggcc tcggtcgtcg
     1501 tgctcgagaa ggcccgattc ccgcgctggc acatcggcga gtcgacgatc cccgccgcca
     1561 acggcgtttt taaagacctc ggcgtatacg acaccctgct gcgctccgac ttcgtcaaga
     1621 agatgggcgt caccttcgtg tggggtcagg accgccagcc gtggtccgcc gattatctga
     1681 ccctgcgcga caccaccggc aaggccgttc ccggcagcgt catcgacgtc accggtcagg
     1741 acttctcggc cctgctcggc ccgggcgcga cccacgatac tccgtactcc gccttcaacg
     1801 tgcgccgcgc cgagttcgat aaactcctgc tcgatcagag ccggcacttc ggcgtcgacg
     1861 tccgcgaggg caccgccgcg cgccaggtcg tccgcggggc cgctggccag gcgcacacga
     1921 tccactggca ggacgaccag gggcacagcg gcgcg
//
LOCUS       DASWNJ010001983         8315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_458114, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001983 DASWNJ010000000
VERSION     DASWNJ010001983.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 8315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8315
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458114"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VGB85_33025"
     CDS             <1..474
                     /locus_tag="VGB85_33025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138972.1"
                     /translation="APFNVLMHLYRWQDLLACFREVRRVLAPGGQFALDVILPDIEWL
                     TWDPNERHSVTYFTHPRTGASMVYSTNHTYDPGTQVCHVRIYYDDAPKRPRAFKPPPE
                     PRQIVHLAHRQIFPEELRMLIATAGLELESLTGNFRSGPLGPGIDAQSAIARKPA"
     gene            764..1507
                     /locus_tag="VGB85_33030"
     CDS             764..1507
                     /locus_tag="VGB85_33030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138973.1"
                     /translation="MRAHLYLLAGLMIAGAGLACDSPSSSDADPRLACLYLGSGTCRP
                     DEPDESTCGYRTETQTTWGAECKGDAPACFRDDHFTELFPEGLYIGCGEYTANVLTAE
                     AVEAALATTGSPRPLLKSEAVAYDGAGDPVVGTALFGEVVALSLNVGFDTVPGFTDGE
                     PAAPLGELVLASGPCAGMDVAHVLDEANLVLGGCPSALTPANAAACVTAINNSFVQPA
                     NTCDDRKKKHQKDDDCEQAPVCSDLFESP"
     gene            1881..2180
                     /locus_tag="VGB85_33035"
     CDS             1881..2180
                     /locus_tag="VGB85_33035"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEY0138974.1"
                     /translation="MQWVNRILLAALLAFAVAYLPRHIYSSGGAEDLGRVEREHQALQ
                     RSNERLRGETAALRAEVAALKRDPAEVARIAREDLGLIRPGEVVFEVQQPVASNQ"
     gene            2177..4321
                     /locus_tag="VGB85_33040"
     CDS             2177..4321
                     /locus_tag="VGB85_33040"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0138975.1"
                     /translation="MTTHLRTLSDLLWRTRWGLVVLASAWILLATGMASSAGFARGSL
                     LLALGLAVYAATLARAVLRELSPGTAARTAQAELELGLLLITGVYLLLALTGGLHSAM
                     HPLVYALVSFLLIVHKTRWVALAWLGAIAALEAIVGMGETRLVIYHLGFIGFFAAGNL
                     LVLGGLVGRLRREHAHRVEGELSRMRQEARDFRLIAGTSGRSREVDELQLAQGAVHGI
                     HEQVAFNLGLLRDALGLHGAALLWMSDDRLVLKQAVGPQDQLCTDLPGPGVLAGIVRD
                     PRALVLKNMGERRLPPYYREERSGEVVTDLCAVPLLRKGELLGIVCADRTGDRPFTAD
                     DQSRISAAAQLVQRIVEQERVFSAIERSKYEQEQFYRASELLSGALGLGEVYDKSFRA
                     IRAIVDYDIAVLTGYDGDALTHRVLAIDTPGARDPEYGVDWGSLADDLHDLVFEDGAG
                     LVAMAVKNRHHMPAGDERVEPETVVFTLHTRLRHAHSLLVLPLVRGDEVLGTITLASS
                     RERKFPRQIREMLRVISHQVGVSLQNARMYRAMEERATTDGLTGLTNHRAFRERLAQV
                     HALAERQGQKFAVILTDIDHFKKVNDTYGHPVGDAVLKRVAAVFAGRARKVDVVARYG
                     GEEFVLILPDTDGAGAEVFANKLREEVAAATMTSEQGPFQVTISMGVAEYPLDTRDAS
                     ELIERADQALYRCKRGGRNCVTRWSRQLAAAS"
     gene            4407..5372
                     /locus_tag="VGB85_33045"
     CDS             4407..5372
                     /locus_tag="VGB85_33045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HEY0138976.1"
                     /translation="MSTAPHDALFKATFSQVERAADELRHVLPPELLARVDLASLSLE
                     SGSFVDEALRERHTDLLFSLRLDGRDARIYLLFEHQSGPDSWMPLRLLRYMLRIWEGC
                     VAAGATLLPVVIPVVLHHGDAGWRAPTRFEALFDLPPEAAEFTPHFRFLLDDLATESE
                     AALRARAASAFTRLVLSALQQSRGERHLGDLLRGWTGLIRELRQAPDGRRALGLIYRY
                     LYEVRGRAELATITAVARENEPENEDFMETIADYLRAQGAARGREEGLREGQRRFLLG
                     MMKNRFGELPEHVRERVMAGDDAELTRWGARLLEARSLDDIFAGP"
     gene            complement(5385..6509)
                     /locus_tag="VGB85_33050"
     CDS             complement(5385..6509)
                     /locus_tag="VGB85_33050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04552 family protein"
                     /protein_id="HEY0138977.1"
                     /translation="MTGKQPDTFSAFITSQALWKGGGLGELSLQDIGEIQLLLRGQSV
                     IDWHRLAMTSEEDVRRLAALNSLDLDDPGDAERVAELRQQAARYIVEVLKLRLDESIA
                     EKTPWVQIPLIASGKGGSLQRQACMLLKVMHIIYHLDARELRTTLAVSDNDIFSLVEE
                     SVTRMFDELRTAGVPVVEFSWSRKTKESQVTKLLLKKETSAARVFDRLRFRLIVRHAA
                     DLVPTLRILLHRCIPFNYVVPGQTVNTLIDLESLKVLHEDGFDTEEGAAPVPDTTVVS
                     GVLPAANEFSGQSFRLLNFIADLPVRVDALLPPGNCQEGRHCVVFVTAEFQIMDKGTA
                     EANEQGENSHSQYKHRQHQHVRVRLLREPRGKPEKGEKNG"
     gene            complement(6585..6761)
                     /locus_tag="VGB85_33055"
     CDS             complement(6585..6761)
                     /locus_tag="VGB85_33055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138978.1"
                     /translation="MSYMSDFFLHRTTRGNQAESNQGLEQGFMTAYLGNICAALVTIG
                     LLVGTLRFSRRVNG"
     gene            7141..7695
                     /locus_tag="VGB85_33060"
     CDS             7141..7695
                     /locus_tag="VGB85_33060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138979.1"
                     /translation="MRDPDDDDDTPPPRDDRMFGTSGFRRKLSDSLTGLLDPDGALGK
                     GKDFVTGVSQATKTEVVRMVSAEVRNFLDGMDTVELLQSVIAGLVIDVKTEIRFSLDP
                     QGQLKTAMTTKESEVRSAHEPKKDAAPPRAPEPPRTTTPEPRRDAVTPRTHENSRPSD
                     SRSDMRSETRSDSRSDSKPGGKPE"
     gene            complement(7730..>8315)
                     /locus_tag="VGB85_33065"
     CDS             complement(7730..>8315)
                     /locus_tag="VGB85_33065"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY0138980.1"
                     /translation="KAHAAEMGNEVPSEPLLFLKPPSCLIASGEPVTLPKGYERIDME
                     AELVVVVGRKATAIQAADAWQHIAGYTLGNDISNRDLQKKDKQWTRAKGFDGFGPVGP
                     WLRMTPRGEVLPAAELRIQGGVNGERKQDAALSDMVFDVPFLMAYISACMTLMPGDII
                     FTGTPEGVSPLTPGASTRVELSGWDMLPLENTLR"
BASE COUNT         1237 a         3025 c         2785 g         1268 t
ORIGIN      
        1 gccccgttca acgtgctgat gcacctgtac cgctggcagg acctgctcgc gtgcttccgc
       61 gaggtgcggc gcgtgctcgc gccaggcggt cagttcgcgc tcgacgtgat cctgccggac
      121 atcgagtggt tgacctggga cccgaacgag cgccacagcg tcacctactt cacgcacccg
      181 cgcaccggcg cgtcgatggt ctactcgacc aaccacacct acgaccccgg cacgcaggtg
      241 tgccacgtcc gcatctatta tgacgacgcg ccgaagcggc cccgggcctt caagcccccg
      301 ccggagccgc ggcagatcgt ccacctcgcg caccgccaga tcttcccgga agagctgcgc
      361 atgctgatcg ccaccgccgg cctcgagctc gagagcctca ccggaaattt ccgcagcggc
      421 cccctcggcc ccggcatcga cgcccagtcg gcgatcgcac gcaaaccggc ctgagctcgc
      481 gtcgacccgc ccgatcgcga gtggcagctt cgcggtgagc ttcgtcagcg gtggcagatt
      541 cgggtccatg tgccacaaaa ctggtgtagc cgcacaaatc gctgcggcgg ctccgcgtcg
      601 ctgcgaacat gacagctgtc cgcatgatca aacccgtctg cgcagcgctt gcgcggctcc
      661 tgagggcatt tcgctgtgtg cccccggcac acgcgtgcac agggtgctac ggggtgagtg
      721 caggtccgtc attttgcgga ccgatgacaa aaggagccca cccatgcgcg cccatctcta
      781 tctcctcgcg ggtctgatga tcgccggggc tggcctcgcc tgcgactctc ccagcagcag
      841 cgatgccgac ccgcgcctcg cctgcctcta cctcggcagc ggcacctgcc gacccgacga
      901 gcccgacgaa tccacctgcg gctaccgcac cgagacccag accacctggg gcgccgaatg
      961 caagggtgac gcacccgcct gcttccgcga cgaccacttc accgagctgt tccccgaggg
     1021 cctctacatc ggctgcggcg agtacaccgc caacgtgctc accgccgagg ccgtcgaggc
     1081 cgcgctcgca accaccggct cgccgcggcc gctgctcaag tccgaggccg tcgcctacga
     1141 cggcgctggc gaccccgtgg tcggcacggc gctgttcggc gaggtcgtcg cgctcagcct
     1201 caacgtcggc ttcgacaccg tccccggctt caccgatggc gagccggcgg ccccgctcgg
     1261 tgagctggtg ctcgcctcgg gtccgtgcgc cggcatggac gtcgcgcacg tgctcgacga
     1321 ggccaacctc gtgctcggcg gctgcccgtc cgcgctgacc ccggccaacg ccgccgcctg
     1381 cgtcaccgcg atcaacaaca gcttcgtgca accggcgaac acctgcgacg accggaagaa
     1441 gaagcaccag aaggatgacg attgcgagca ggcccccgtc tgcagcgacc tcttcgagtc
     1501 gccctgagga gctcatgtcc gaggtcgcgc ccttccccga ggcgaacgtc tggtcgaccc
     1561 cgatccacga cctcgggctg acgatcgccg gctgctggcc gccctgaatc ccggtggcat
     1621 cgagcacgcg cctggcgcct gagttggtcc tctgcccctc ggttcgcatg cgcgagcacg
     1681 gccacctgct gatcggcgag gcccgctgcg gcacaccggg gggtccggga gctcgacggg
     1741 cgccttcagg acttcaccgt ccagttcgtc ggcccgacga gggcgacccc cgggcgcgcc
     1801 gcgctcgcgg gacccgcgct cggcgaaacc gccgaaatgt tgcgcccacg gccgccaggg
     1861 ataccgtgcg gacctctcgc atgcagtggg tcaaccgcat cttgctcgcc gccttgctgg
     1921 ccttcgccgt ggcctacctg ccgcggcaca tctacagcag cggcggcgcc gaggacctcg
     1981 gccgcgtcga gcgtgaacac caggccctgc aacgcagcaa cgagcgcctg cgcggcgaga
     2041 ccgcggctct gcgggccgag gtcgccgcgc tcaagcgtga ccccgccgag gtcgcccgca
     2101 tcgcccgcga ggacctcggc ctgatccgtc cgggcgaggt cgtgttcgaa gtccagcagc
     2161 ccgtagcgag caaccaatga cgactcacct gcgtaccctc tcggacctgc tctggcgcac
     2221 gcgctggggc ctcgtcgtgc tcgcctcggc gtggatcctg ctggccaccg gcatggcctc
     2281 gtcggccggc ttcgcccgcg gcagcctcct gctcgcgctc ggcctcgccg tgtacgcggc
     2341 gacgctcgcc cgcgcggtgc tacgcgagct gtccccgggg acagccgcgc gcaccgccca
     2401 ggccgagctc gagctcggcc tgctgctgat caccggcgtg tacttgctgc tcgcgctgac
     2461 cggcggcctg cacagcgcga tgcacccgct ggtctacgcg ctggtctcgt tcctgctgat
     2521 cgtccacaag acgcggtggg tcgcgctcgc ctggctcggc gcgatcgctg cgctcgaggc
     2581 gatcgtcggc atgggcgaga cgcggctggt catctaccac ctcggcttta tcggcttctt
     2641 cgccgccggc aacctgctgg tgctcggcgg cctcgtcggt cgcctgcgcc gcgagcacgc
     2701 gcatcgcgtc gagggcgagc tctcgcgcat gcgccaggag gcccgcgact ttcgcctgat
     2761 cgccggcacc agcggccgca gccgcgaagt cgatgagctc cagctcgcgc agggcgccgt
     2821 gcacgggatc cacgagcagg tcgcgttcaa cctcggcctc ctgcgcgacg ccctaggctt
     2881 gcacggcgcc gccctgctgt ggatgtcgga cgaccgcctc gtgctcaagc aggccgtcgg
     2941 cccgcaggac cagctgtgca ccgacctccc gggccccggc gtgctcgccg gcatcgtccg
     3001 cgacccgcgg gcgctggtgc tcaagaacat gggcgagcgg cgcctgccgc cctactaccg
     3061 cgaggagcgc agcggcgagg tcgtcaccga cctgtgcgcc gtgccgctgc tgcgcaaggg
     3121 cgagctgctg ggcatcgtct gcgccgaccg cacgggcgac cgtccgttca ccgccgacga
     3181 ccagtcgcgg atctccgccg ccgcgcagct ggtccagcgg atcgtcgagc aggagcgcgt
     3241 gttctcggcg atcgagcgca gcaagtacga gcaggagcag ttctaccgcg cgagcgagct
     3301 gctcagcggc gcgctcgggc tcggcgaggt ctacgacaag agctttcgcg cgatccgtgc
     3361 gatcgtcgac tacgacatcg ccgtgctgac cggctacgac ggcgacgccc tgacccaccg
     3421 cgtgctcgcg atcgacaccc ccggcgcgcg cgacccggag tacggcgtcg actggggcag
     3481 cctcgccgac gacctgcacg acctcgtgtt cgaggacggg gccggtctgg tcgccatggc
     3541 cgtcaagaac cggcaccaca tgcccgccgg cgacgagcgc gtcgagcccg agacggtcgt
     3601 gttcacgctg cacacgcggc tgcgtcacgc gcacagcctg ctggtgttgc cgctggtccg
     3661 cggtgatgaa gtgctcggca cgatcaccct cgcctcctcg cgcgagcgca agttcccgcg
     3721 acagatccgc gagatgctgc gcgtcatctc gcatcaggtc ggcgtcagcc tgcaaaacgc
     3781 ccgcatgtat cgggcgatgg aggagcgcgc gaccaccgac ggcctcaccg gcctcaccaa
     3841 ccatcgcgcc ttccgcgagc gcctcgccca ggtccacgcg ctcgccgagc gccagggcca
     3901 gaagttcgcc gtgatcctga ccgacatcga ccattttaaa aaggtcaacg acacctacgg
     3961 gcaccccgtc ggtgacgcgg tgctcaagcg ggtcgccgcg gtgttcgccg ggcgcgcgcg
     4021 caaggtcgac gtcgtcgccc gctacggcgg cgaggagttc gtcctgatcc tgcccgacac
     4081 ggatggcgcc ggcgccgagg tgttcgccaa caagctgcgc gaggaggtcg ccgcggcgac
     4141 gatgaccagc gagcagggcc cgttccaggt cacgatctcg atgggcgtcg ccgagtaccc
     4201 gctcgacacc cgcgacgcga gcgagctgat cgagcgcgcc gaccaggccc tttatcgctg
     4261 caagcgtggc ggccgtaact gcgtcacccg ctggtcgcgt cagctcgccg cggccagctg
     4321 accaccgcga cgcgggtggc caacccgcgg gacactcggc gcgccggagt tgcatcgact
     4381 ccggcgcgcg ctagtttgcg cggccgatgt cgaccgcgcc gcacgacgct ctgttcaagg
     4441 cgaccttcag ccaggtcgag cgcgccgcgg acgagctgcg ccatgtactc ccgccggagc
     4501 tgctcgcgcg cgtcgacctt gcgagtctgt ccctcgagtc aggcagcttc gtcgacgagg
     4561 cgctgcgcga gcgtcacacc gacctgctgt tctcgctgcg cctcgacggc cgcgacgccc
     4621 gcatctacct gctgttcgag caccagagcg gcccggactc gtggatgccg ctgcgactgc
     4681 tgcgctacat gctgaggata tgggagggct gcgtcgccgc gggggcgacg ctgctgccgg
     4741 tcgtcatccc ggtcgtgctg caccacggcg atgccggctg gcgcgcgccg acccgcttcg
     4801 aggcgctgtt cgacctaccg cccgaggccg cggagttcac cccgcacttt cgcttcctgc
     4861 tcgacgacct cgccaccgag agcgaggctg ctctgcgcgc acgggcggcg tccgcgttca
     4921 cgcgcctggt gctgtcggcg ctgcagcagt cccgcggcga gcggcacctc ggcgacctgc
     4981 tgcgcggctg gaccggcctg atccgcgagc tgcggcaggc gcccgacggc aggcgtgcgc
     5041 tcgggctgat ctaccgctat ctttacgagg tccgcgggcg ggcagagctc gccactatca
     5101 ccgccgtcgc ccgcgagaac gaaccggaga acgaggactt catggagacc atcgcagatt
     5161 atctgagggc gcaaggagcg gcccgcggcc gcgaggaggg cctccgcgag ggccaacgcc
     5221 ggttcctcct cggcatgatg aagaaccgct tcggcgagct cccggagcac gtccgcgaac
     5281 gcgtcatggc cggggacgac gccgaactca cccgctgggg cgcccgcctg ctcgaggccc
     5341 gcagcctcga cgacatcttc gccgggccct gaggctgcgc gcggctagcc gttcttctcg
     5401 cccttctcgg gcttgcctcg cggctcccgc agcagtcgca cacgcacgtg ctggtgctgg
     5461 cggtgcttgt actgcgagtg cgagttctcg ccctgctcgt tggcctcggc cgtgcccttg
     5521 tccatgatct ggaactcggc ggtgacgaac acgacgcagt ggcggccctc ctggcagttg
     5581 ccgggcggca gcagggcgtc gacgcgcacc ggcaggtcgg cgatgaagtt gagcaggcga
     5641 aagctctggc ccgagaactc gttggcggcg ggcagcacgc cgctgaccac cgtggtgtcg
     5701 ggcacggggg cggcgccctc ctcggtgtcg aagccgtcct cgtggagcac cttgaggctc
     5761 tcgaggtcga tcagcgtgtt caccgtctgg ccgggcacca cgtagttgaa cgggatgcag
     5821 cggtgcagca ggatccgcag ggtcgggacc aggtcggccg cgtggcggac gatcaggcga
     5881 aagcgcaggc ggtcgaacac ccgggccgcg ctggtctctt ttttgagcag cagcttggtg
     5941 acctgcgact ccttggtctt gcgcgaccac gagaactcga ccaccggcac gcccgcggtg
     6001 cgcagctcgt cgaacatgcg cgtcacgctc tcctcgacga gcgagaagat gtcgttgtcg
     6061 gacaccgcga gggtcgtccg cagctcgcgg gcgtcgaggt ggtagatgat gtgcatcacc
     6121 ttgagcagca tgcaggcctg gcgttgcagg ctgccgccct tgcccgaggc gatcagcggg
     6181 atctgcaccc acggggtctt ttcggcgatg ctctcgtcga ggcgcagctt gagcacctcg
     6241 acgatgtagc gcgcggcctg ctggcgcagc tcggcgacgc gctcggcgtc gccggggtcg
     6301 tcgaggtcga ggctgttgag cgcggcgagg cggcgcacgt cctcctcgct ggtcatcgcc
     6361 aggcggtgcc agtcgatcac tgactgtccg cgcagcagca gctggatctc gccgatgtcc
     6421 tgaagactga gctcgcccag tcctccgccc ttccacagcg cctgtgacgt gatgaaggcg
     6481 ctgaaagtgt cgggctgctt gccggtcatt ctcgaggact cgcaggccgc cgacgctgtc
     6541 ggcggctgat tcagctgatt cagccgttca cccgagcacc cggatcagcc gttcacgcgc
     6601 cgcgagaagc gcagcgtgcc gacgagcaga ccgatggtga cgagagcggc acagatgttt
     6661 ccgaggtatg cggtcatgaa cccttgctcc agtccttgat tggactctgc ctggttacca
     6721 cgcgtagtgc ggtggaggaa aaagtcggac atgtaggaca aggagtaagg gtaagtccga
     6781 gatttcccgg gttgcccctg gggacaaatc ccgtatctcg aacgtccgga cccagttagc
     6841 acattcccgg cgggcttgga aagcccccac aagccgctaa atcccggcag aactgccggg
     6901 aacccatgca cctacactcc tcaaagtggc gtcaggagcg gtgtcccggg cagattgcga
     6961 ggtggcccgg gatccatcgc gcaggcgcgc acggcgggcg cagcggctcg acccttgccg
     7021 caggaacttc cgggagatct accgcgaaag ggcctcctgg ggacgatttg gccggtgcac
     7081 cccacgccca cagggcgtga attgtggcct cctgcgccca gcggcggtac cgtgccgggg
     7141 atgcgcgacc cggacgacga cgacgacacc ccgccgcccc gcgatgaccg gatgttcggg
     7201 accagcggtt ttcgccgcaa gctcagcgac agcctcactg gcctgctcga ccccgacggc
     7261 gcgctcggca agggcaagga cttcgtcacg ggtgtctcgc aggcgaccaa gaccgaggtc
     7321 gtccgcatgg tctcggcgga ggtccgcaac ttcctcgacg gcatggacac cgtcgagctg
     7381 ctgcagagcg tcatcgccgg cctcgtcatc gacgtgaaga cggagatccg gttctcgctc
     7441 gacccgcagg gccagctcaa gacggcgatg acgaccaagg agtcggaggt ccgcagcgcc
     7501 cacgagccca aaaaggacgc cgccccgccg cgcgccccgg agccgccgcg caccaccacg
     7561 cccgaacctc gccgcgatgc cgtcacgccg cgcacccacg agaactcgcg cccctccgac
     7621 tcgcggtcgg acatgaggtc ggagaccaga tccgacagca ggtccgacag caagcccggc
     7681 ggcaagcccg agtgagctgt ccttcggggc agcccaggcc ggcgccgcgc tagcgcagcg
     7741 tgttctcgag cggcagcatg tcccagccgg acagctcgac ccgcgtgctc gcgccggggg
     7801 tcagcggcga gacgccctcg ggcgtgccgg tgaagatgat gtcgcccggc atcagcgtca
     7861 tgcaggcgct gatgtacgcc atcaggaacg gcacgtcgaa gaccatgtcg gacagcgccg
     7921 cgtcctgctt gcgctcgccg ttgacgccgc cctggatgcg cagctcggcg gccggcagga
     7981 cctcgccgcg cggggtcatg cgcagccacg gtccgacggg gccgaagccg tcgaagccct
     8041 tggcgcgcgt ccactgcttg tccttttttt gcaggtcgcg gttgctgatg tcgttgccga
     8101 gcgtgtagcc ggcgatgtgc tgccaggcgt cggcggcctg gatcgcggtc gccttgcggc
     8161 cgacgacgac caccagctcg gcctccatgt cgatgcgctc gtaacctttc gggagcgtga
     8221 ccggctcacc gctggcgatc aggcagctcg gcggcttgag gaacagcagc ggctcgctcg
     8281 ggacctcgtt gcccatctcg gccgcgtgcg ccttg
//
LOCUS       DASWNJ010001984         1956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_387345, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001984 DASWNJ010000000
VERSION     DASWNJ010001984.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387345"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..744)
                     /locus_tag="VGB85_33070"
     CDS             complement(28..744)
                     /locus_tag="VGB85_33070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138981.1"
                     /translation="MTGATTSGAPQAAPRVLVAHERPVLARAIQQVLAAQGMQVEVML
                     TGDAVARALQLQTWDALVLDVGLPGPPVHQLATLAKTGGSTAVKALVLVTSVFRRGSY
                     KRPPQQLYGADDYVEVHQLGAKLPGKLWRLLAGDAAGPDGMLEAEALLIGLQASAGGD
                     TLPRLATLLVADAVLHNGDRLADADTPEQARDALASELAQARGQYHEVVGATPNEVDP
                     IDAAFDALVRGRGEEAGAWT"
     gene            complement(741..1256)
                     /locus_tag="VGB85_33075"
     CDS             complement(741..1256)
                     /locus_tag="VGB85_33075"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY0138982.1"
                     /translation="MSELDRDGRAAGLEDDLKLAGFYVGAGLYGIEIMRIKEVIQART
                     LVVRPVPFAPAIVEGVIQLRGVVIPVVDLRRRFETPIDPSIEHLNKLIIVSVAGRIAG
                     LKVDRVLGELRVAASSLRPAPSMLRPDGERGTENVFSGVSRITRDKSEVAEMVFVINL
                     DVLLRGAGARS"
     gene            complement(1253..1885)
                     /locus_tag="VGB85_33080"
     CDS             complement(1253..1885)
                     /locus_tag="VGB85_33080"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY0138983.1"
                     /translation="MSVEREDLWQALLRSGETIATDEDYEHGYKRDVPQDLQQYVSFR
                     VSGEIYGLPIHEIEEISKVFGTTHVPRTADFLVGIGNVRGRIMPVVDLAKRLRLRPME
                     RGRAARVLIVHLNDEPYGLVVDAVIDVIRIPPDKIEERPGGITGTRAEYIRGLGRRKG
                     VAGPGKGLGGAEMKAGEEPEEIVIILNLATLLDPKDFVRVGAGTAEEGRG"
BASE COUNT          258 a          675 c          730 g          293 t
ORIGIN      
        1 gttgctcgag gtcgggcggg agagcggtca cgtccacgcg cctgcttcct cgccgcggcc
       61 gcgcaccagc gcgtcaaaag cggcgtcgat cgggtccact tcattcggcg tcgcgccgac
      121 gacttcatgg tactggccgc gcgcctgcgc gagctccgac gcgagcgcgt cgcgggcctg
      181 ctcgggcgtg tcggcgtcgg cgaggcggtc gccgttgtgc agcacggcgt cggcgacgag
      241 cagggtcgcc aggcgcggca aggtgtcacc ccccgcgctc gcctggagac cgatgagcag
      301 cgcctcggcc tcgagcatgc cgtcggggcc ggcggcgtcg ccggcgagca ggcgccacag
      361 cttgcccggg agcttcgcgc cgagctggtg gacctcgacg tagtcgtcgg cgccgtagag
      421 ctgctgcggc ggccgcttgt agctgccgcg gcggaacacg gaggtgacga gcacgagcgc
      481 cttgacggcc gtgctgccgc cggtcttggc gagcgtggcg agctgatgga ccggcgggcc
      541 cggcaggccg acgtcgagca cgagggcgtc ccaggtctgg agctggaggg cgcgcgcgac
      601 cgcgtcgccg gtcagcatga cctcgacttg catgccctgc gccgcgagca cctgctggat
      661 cgcgcgcgcg aggacggggc gctcgtgggc gacgagcacc cgcggggccg cctgcggcgc
      721 gcctgacgtg gtcgcgccgg tcatgatcga gcccccgcgc cgcgcagcag cacgtcgagg
      781 ttgatgacga acaccatctc cgcgacctcc gacttgtccc gggtgatccg gctgacgccg
      841 ctaaacacgt tctcggtgcc ccgctcgccg tcggggcgca acatcgaggg cgccggccgc
      901 aggctgctcg cggcgacgcg cagctcgccg agcacgcggt cgaccttgag gccggcgatc
      961 cggccagcga cgctgacgat gatcagcttg ttgaggtgct cgatcgacgg gtcgatcggg
     1021 gtctcgaagc ggcgccggag atcgacgacg gggatcacga cgccgcgcag ctggatgacg
     1081 ccctcgacga tcgccggcgc gaaggggacc gggcgcacga ccagcgtacg cgcctggatg
     1141 acctccttga tgcgcatgat ctcgatgccg tacaggccgg cgccgacgta gaagccggcc
     1201 agcttgaggt cgtcctcgag gcccgcggcc ctgccgtcgc ggtcgagctc gctcatcccc
     1261 tgccctcctc ggccgtgccg gcgccgacgc gcacgaagtc cttggggtcg agcagggtcg
     1321 cgaggttgag gatgatgacg atctcctcgg gctcctcgcc ggccttcatc tcggcgccgc
     1381 cgaggccctt gcccgggccg gcgacgccct tgcggcggcc gaggccgcgg atgtactcgg
     1441 cgcgcgtgcc ggtgatgccg ccggggcgct cctcgatctt gtcgggcggg atccggatga
     1501 cgtcgatgac ggcgtcgacg acgaggccgt agggctcgtc gttgaggtgg acgatcagca
     1561 cgcgggccgc ccgaccgcgc tccatcgggc gcaggcgcag gcgcttggcg aggtcgacga
     1621 cgggcatgat gcggccgcgg acgttgccga tgccgaccag gaagtcggcg gtgcgcggga
     1681 cgtgggtggt gccgaagacc ttgctgatct cctcgatctc gtggatcggc aggccgtaga
     1741 tctcgccgct gacgcggaag ctgacgtact gctggaggtc ctgcgggacg tcgcgcttgt
     1801 agccgtgctc gtagtcctcg tcggtggcga tcgtctcgcc gctgcgcagc agcgcctgcc
     1861 acaggtcctc gcgctcaacg gacattgttc accccgaagg tgttcacccc gaaggtgttc
     1921 accccgatgt tactcacccc gatcgagatc gcagcg
//
LOCUS       DASWNJ010001985         3839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_418148, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001985 DASWNJ010000000
VERSION     DASWNJ010001985.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418148"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="VGB85_33085"
     CDS             <1..998
                     /locus_tag="VGB85_33085"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEY0138984.1"
                     /translation="AMFLPRVGHEVLVDFIEGDPDRPVVTGRVYHGDNKPPYPLPADK
                     TKTTIKSDSSIGGGGFNELRFEDKKGAEEVFLHAQKDLNEVVLNDNSRTVTANQTFSV
                     GGNQSFSITKNRSVTVTEGDESLTVTKGKSSTTVKQDRSVTVQSGNSSMTVETGSRSV
                     TVKQAITEKSTDANIDLTAHTQMSLKAETATVSVTAETDLSLTSNTATLVAMASKEVS
                     LVSTAASLSAQGLTGVSITSLAGPISLLSLQQTSVTSLANVLITGPLGVQVNGGAEVG
                     IAAKKIVLTGGEEVTLQVGSSAITIRADGITISGSKITSTAVGMHEISGALIKIN"
     gene            1011..2195
                     /locus_tag="VGB85_33090"
     CDS             1011..2195
                     /locus_tag="VGB85_33090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138985.1"
                     /translation="MRPLRELRGELAGFLGDQDHGVCVLRGGREYLPFVYKILQELET
                     TATDVFLSFPHRFESAAGYAELIARRIEESEREARGATALELRHERPVADRIKDALGH
                     ARELLPRGHDMPRLVVILVPLEIADERAYTDLVHALTEPGGLPPWFRRMRIFMHVAGT
                     DAWPLPRFVRSLTVDLSAEELARGAVEDAGDPSLTRQQRAQALLQAAMLDVGHRRYEA
                     ARGRLDAVHGESQALGSPVLAALALSGLGDIERIERRNEDAIGWYERALVPASEAGAP
                     VVLLMITRHLADLYFAGGRLADAEVFFDGAQQLARVLPEPETQATSLLGRGLAQQRRG
                     APAAVWAASFVAAAEVARDNDRTQLLVEIRPHLHASRTQALTPELRRSIDTMLGGGHE
                     RR"
     gene            2182..3078
                     /locus_tag="VGB85_33095"
     CDS             2182..3078
                     /locus_tag="VGB85_33095"
                     /inference="COORDINATES: protein motif:HMM:NF017315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HEY0138986.1"
                     /translation="MSGGSQVMRAAKAERPPSPARTLQQGIGKAMQIISAPTDAINMG
                     IAKLTDGISKALPSFPAATIGSLVLALPHGHPHPPSFGVPLPPIGAVMAGCCVSVLIG
                     NLPAARCGDIGMSPTCLGYLPGFEIFTGSSKVFIGGARAARAIDITMHCWPPKGWLAR
                     GAIAALTKAAMAGMKVMDVAGKVAMTAGVVADLEDAGTSAAAGDSAMASASLLSAGMA
                     VAQAAADAASAALGAVIGKDLGVGPPVGAITLGNPSVLIGGFPMPSGMQIAMRKLTSA
                     NFEPKKRGKNDDNTTGCKECGK"
     gene            3093..>3839
                     /locus_tag="VGB85_33100"
     CDS             3093..>3839
                     /locus_tag="VGB85_33100"
                     /inference="COORDINATES: protein motif:HMM:NF040127.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
                     /protein_id="HEY0138987.1"
                     /translation="MGDGVRWVGDPVDVVTGALVETAAEFTIAGPLPIVWQRHYSTAR
                     VEMAGPLGWGHTHGHDRTLRAAVDGWLVRQADGESLLFPYDALTQPQRGWRLLQRATE
                     QHLRAPDGTVYVFPTGKLDMVARPEQMIRGAHALAFVYDREGRIIEISDRGRARVVAV
                     EYAAGRISALISVAHPSREDRRLAMMRYEYDARGDLVAATDRYGHRRTFAYDAAHRMV
                     ARTDRNGYRFEYEYDNEGRCVASRGHDGVQA"
BASE COUNT          635 a         1275 c         1373 g          556 t
ORIGIN      
        1 gcgcgatgtt cttgccgcgc gtcggccacg aggtgctcgt cgacttcatc gagggcgacc
       61 ctgaccgtcc ggtcgtcacc ggtcgtgtct atcacggcga caacaagccc ccctacccgc
      121 tgcccgccga caagaccaag acgacgatca agtccgactc ttcgatcggg ggtggcggct
      181 tcaacgagct gcgcttcgag gacaaaaagg gcgccgagga ggtgttcctg cacgcccaga
      241 aggacctcaa cgaggtcgtc ctcaacgaca acagccgcac cgtgaccgcc aaccagacct
      301 tctccgtcgg cggcaaccag agcttctcga tcacgaagaa ccgcagcgtg acggtgacgg
      361 agggcgacga atcgctgacg gtcacgaagg gcaagagctc gacgaccgtg aagcaggacc
      421 gcagcgtgac ggtgcagtcc gggaacagct cgatgaccgt ggagaccggc tcgcgctcgg
      481 tgaccgtcaa gcaggcgatc acggagaagt cgaccgacgc gaacatcgac ctgacggcgc
      541 atacacagat gagcctcaag gccgagacgg cgaccgtgtc ggtgaccgcg gagactgatc
      601 tatcgctgac gtcgaacacg gcgacgctcg tggcgatggc ctcgaaggag gtctcgctgg
      661 tgtcgacggc ggcctcgctg tcggcccagg ggttgaccgg tgtgtcgatc acgtcgctcg
      721 ccggtccgat ttccctcctg agcttgcagc agacgtccgt gacgtcgctg gcgaacgtgc
      781 tgatcaccgg ccccctcggg gtccaggtca acggcggcgc cgaggtcggc atcgcggcca
      841 agaagatcgt cctcacgggc ggcgaggagg tcacgctgca ggtcggcagc agcgcgatca
      901 cgatcagagc cgacgggatc accatctcgg gctcgaagat cacgagcacg gcggtcggca
      961 tgcacgagat ctcgggcgcg ctgatcaaga tcaactgagt cgggaacacc ttgcgtccac
     1021 tgcgtgagct tcgaggcgag ctggcgggct tcctgggcga ccaggaccac ggggtatgcg
     1081 tgctccgcgg tggccgcgaa tatctgccgt tcgtctacaa gatcctgcag gagctcgaga
     1141 cgacggcgac ggacgtgttc ctgagcttcc cacaccgctt cgagagcgcc gcgggctatg
     1201 ccgaattgat cgcacgacgg atcgaggaga gtgagcgcga ggcccgtggt gccacggccc
     1261 tggagctccg ccacgagcgc ccggtggccg accggatcaa agatgccctc ggacacgcac
     1321 gcgagctgtt gccccggggg catgacatgc cgcgactggt cgtcattctc gtcccgctgg
     1381 agatcgccga cgagcgtgca tataccgact tggtccacgc gttgaccgag ccaggcggcc
     1441 tgcccccgtg gtttcgtcgg atgcggatct tcatgcacgt cgcgggcacc gatgcctggc
     1501 cgctgccacg cttcgtgcga tcgctgacgg tcgatctgtc cgccgaggag ctggcgaggg
     1561 gcgccgtcga ggacgccgga gatccgtcgc tgacgcggca gcagcgggcc caggcgctgc
     1621 tgcaggcggc gatgctcgat gtcggtcacc ggcgctacga ggccgcgcga gggcgcctcg
     1681 acgccgtcca tggcgaatcg caggcgctcg ggagcccggt gctcgccgcg ctggcgctga
     1741 gcgggctcgg tgacatcgag cgcatcgagc gacgcaacga ggacgcgatc ggctggtatg
     1801 agcgagcgct cgtccccgcg agtgaggcag gcgcgccggt cgtactgctg atgatcacgc
     1861 ggcacctcgc cgacctctac ttcgccgggg gccggctcgc cgacgccgag gtgttcttcg
     1921 acggcgcgca gcagctggca cgtgtgctcc ccgaacccga gacgcaggcg acctcgctgc
     1981 tcggccgtgg gctggcccag cagcggcgcg gcgccccggc tgcggtgtgg gcggcgagct
     2041 ttgtggcggc cgccgaggtc gcccgcgaca acgatcgtac gcagttgctg gtcgagatcc
     2101 gcccgcatct tcacgcgagc cggacccagg cattgacccc cgagctgcgc cggtcgatcg
     2161 acacgatgct gggaggtggg catgagcggc ggtagccagg tgatgcgggc cgccaaggcc
     2221 gagcgaccgc cgagccccgc gcgcacgctg cagcagggga tcggcaaggc gatgcagatc
     2281 atcagcgcgc cgaccgacgc gatcaatatg gggatcgcca agttgaccga tgggatctcg
     2341 aaggcgctgc cgtcgtttcc ggcggcgacg atcggctcgc tcgtgctcgc gctcccgcac
     2401 ggtcacccgc acccgccgag cttcggcgtg cccctgccac cgatcggggc cgtgatggcc
     2461 gggtgctgcg tgtcggtgct gatcggcaac ttgccggcgg ctcgctgcgg cgatatcggc
     2521 atgtccccga cgtgcctcgg gtacctcccg gggttcgaga tcttcaccgg ttcatcgaaa
     2581 gtgttcatcg gcggcgcgcg agcggcccgg gccatcgaca tcaccatgca ctgctggcct
     2641 cccaagggct ggctggcccg cggcgcgatc gctgcgctga ccaaggcagc gatggcgggg
     2701 atgaaggtca tggacgtcgc cggcaaggtc gccatgacgg ccggggtcgt cgcggacctc
     2761 gaggacgccg gcaccagcgc agccgcgggg gactccgcga tggcctcggc gagcttgctc
     2821 tcggccggaa tggccgtcgc acaggcggcc gcggacgcgg cctcggccgc gctcggcgct
     2881 gtgatcggga aagacctcgg cgtcgggccg cccgtcgggg ccatcacgct cgggaacccg
     2941 agcgtattga tcggcggctt cccgatgccc agcggcatgc agatcgcgat gcgcaagctg
     3001 accagcgcaa acttcgagcc aaaaaagcgc ggcaagaacg acgacaacac gacggggtgt
     3061 aaggagtgcg gaaagtagac gcgagcgggt gcatgggcga cggcgtacgc tgggtcggcg
     3121 atcccgtcga cgtcgtgacc ggggcgctcg tcgagactgc cgcggaattc acgatcgcgg
     3181 ggccgctgcc gatcgtctgg cagcgccatt acagcaccgc gcgcgtcgag atggcgggac
     3241 cactcgggtg ggggcacacg cacgggcacg accgcacgct gcgggccgcc gtcgacggct
     3301 ggctcgtccg gcaagcggac ggcgaatcgt tgctgttccc ctatgacgcg ctcacgcagc
     3361 cccagcgagg gtggcggctg ctgcagcgcg cgaccgagca acatctgcgc gcgccggacg
     3421 ggaccgtgta cgtgtttccg acgggcaagc tggacatggt ggcgcggccc gagcagatga
     3481 tccgcggcgc gcatgcgctg gccttcgtgt atgaccgcga gggccgcatc atcgagatca
     3541 gcgatcgtgg gcgcgcgcgc gtcgtcgcag tggagtacgc cgcgggccgg atctccgcgc
     3601 tgatatcggt ggcccatccg tcacgcgagg accgacggct ggcgatgatg cgttacgagt
     3661 acgacgcgcg cggcgacctg gtagccgcca ccgaccgtta cgggcaccgg cggaccttcg
     3721 cctacgacgc ggcgcaccga atggtcgcgc ggaccgaccg caacggctac cgtttcgaat
     3781 acgagtacga caacgagggc cgctgcgtcg cgtcacgcgg gcacgacggc gtgcaggcg
//
LOCUS       DASWNJ010001986         5641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_140425, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001986 DASWNJ010000000
VERSION     DASWNJ010001986.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5641
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140425"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VGB85_33105"
     CDS             complement(<1..416)
                     /locus_tag="VGB85_33105"
                     /inference="COORDINATES: protein motif:HMM:NF017739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HEY0138988.1"
                     /translation="MSISDTIAEFAQDLLPTVQYGFTSKDTSDVHWQVRRFSLHESLS
                     APYRLEIELWTEDLGVQPTGLLGDSCTLTIERDGKGRRVHGIIHHVDMLPAVLERMRV
                     RVVAAPALHLLTYQRNTRFWQEKTALEILDEVLGPAL"
     gene            946..1839
                     /locus_tag="VGB85_33110"
     CDS             946..1839
                     /locus_tag="VGB85_33110"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY0138989.1"
                     /translation="MSLMSQKDAVSILGLGQMGAALARAYIDAGHRVTVWNRSPGKAE
                     PFHGQAKVAETPAQACSEGDLTIVSVSNYQASDEVLRTPLVAQAMKGRTIVQLTSGTP
                     GDARSGAAWASEHGIDYLDGAILAYPSYIGGETTTVFYSGPRAVYDRHEATLRVIGGG
                     TSHVGEPIGAAATLDCALLESYYGATLSFLHGAAICASEKFPLDAYFAGVQAIMPLNA
                     ITVDMCKKMLASGDFKGTDCTLDIHTGAIEHIVRLSQENSVDRRIPELILSYFKRALQ
                     LGHGADEMAAVFNAIKDPSRG"
     gene            complement(1917..2666)
                     /locus_tag="VGB85_33115"
     CDS             complement(1917..2666)
                     /locus_tag="VGB85_33115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138990.1"
                     /translation="MFRDTVRLVLLGALLAACSPGKGGSEDSGESESGTETGATTGTP
                     TTGGTATEPTTGAAACEEYLSSADFGPSLQLTVQYKTGTEPVWIEAETCAGLPHILLS
                     NLLGQPLLQTTSDGSSKLCDELLSAGSECEVGGEDCAAPTLLRLDPGASITITRPAIQ
                     YVEMEMTAECAPGTNCQGACLRAEPLAAGKYAVGAFPFSTCVGACECDAPPVNGWCRV
                     FGGEPGEMLSVFATELVYPDSMEATVVIEVP"
     gene            complement(2752..4122)
                     /locus_tag="VGB85_33120"
     CDS             complement(2752..4122)
                     /locus_tag="VGB85_33120"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEY0138991.1"
                     /translation="MKIVQPRFFDGRLGRRRLLQSLGVSAALAPFIPMLARGEDVNPP
                     RRLILVYTPHGTIHDMWTPTGSETNFTLPELLAPFEPYRADNLIVLKGLQLLGQGGAG
                     PHTRGMPLLFTGSEQLPGDKYKHGGAPPDDPLGWGGNVNASIDQVIAANTLGVTALPS
                     LHLGYQAGGLSVANRMSFTAPDTPTELFQDPQVAWQQLFGLASLDKAAYERLRTKRMS
                     SIDVVRAELSGLQTRIGAEERPKIDSHLQALQDIETKLQNGYTCEVPETPAPMQAFDA
                     NEASSEVLSRHIELAVAALRCDLTRVVTLQVARGEWDANTYPWISGPPADFHHAHSHN
                     EAPGNEVQAFLVKIRRYYAEKIRELADALAAVPEGDGTMLDNTMIVWGSEVANGFSHS
                     FKQMPFVILGGKKFLRTGRFSTHEDVAHHRLLVGILHAMGQTHIDNYGDNDVEAGSGP
                     LPGLLL"
     gene            complement(4133..>5641)
                     /locus_tag="VGB85_33125"
     CDS             complement(4133..>5641)
                     /locus_tag="VGB85_33125"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEY0138992.1"
                     /translation="PLRRLTRAQYDYTIQDLLQVGSVPTDALAGLDAKVGTFTSNIGA
                     PLTVIGVSSLTDIAEQIAGVADLTLLVPCDPAALSPDAPELASCVRDFIDTFGSRALR
                     HPLTEEEFARYLGVYETTLALTEETPGTALRQVIATMLQSPYFLYQVELGRAAPDARG
                     WAALTDHELAARLSYFLWSSMPDDALRAAADAGTLADDAVLLAQLERMLYDDRASRGI
                     GAFVVQWLELDHLDWTEKSPHPLVPELTPELRAAMKREAVDFMTAVMRDDDGRVETML
                     TAPYSLVPPELYPLYGVEAPAEPNAPVQLDPARRAGLLTQVGWLTTHANPGQSSPVLR
                     GVVVLESLLCTYLPAPPNDIKPPPPDPNATTRERFEVHTSVPSCQGCHTFIDGIGFGF
                     EGYDQYGVWRELENGEPIDESGVLRSYGTGEQPFVGPVELAKLLATEDSVRDCVAFQW
                     TTYALGRILSTTDTCQVDDLQQRFAGHDGDMRALLTDIVTSPAFRTLVVATP"
BASE COUNT          859 a         1895 c         1993 g          894 t
ORIGIN      
        1 agcgccggcc cgagcacctc gtcgaggatc tccagcgcgg tcttctcctg ccagaagcgg
       61 gtgttgcgct ggtacgtcag caggtgcaag gccggcgccg cgaccacgcg cacgcgcatg
      121 cgctcgagca ccgctggcaa catgtcgacg tgatgaatga tgccgtgcac gcggcgaccc
      181 ttgccgtcac gctcgatcgt cagcgtgcag ctgtcaccga gcaggccggt cggctgcacc
      241 ccgaggtcct cggtccacag ctcgatctcg aggcggtacg gcgcggacag cgactcgtgc
      301 aggctgaagc ggcggacctg ccagtgcacg tcggacgtgt ccttcgaggt gaagccgtac
      361 tggaccgtcg gcaagaggtc ctgggcgaac tcggcgatcg tgtcggagat ggacatgaac
      421 gtatcctcga gcgacgtgaa gcgacgacct cggcgcgccg agccccacgg ccacacgggc
      481 gacgtcagcg catatcttcg cgggccagac gctatcattc gcattccaat gcttcaagcc
      541 tcgatcccgc cccgagttcc gcgcatcgcc gtatccgcgc gtgctggcgc gcgatgatcg
      601 acgcacgtcg ggcgctggcg agcgcgccgt gttcggcggg atcgggtggg ggcgagcggg
      661 ggcaggccgt gctcgtggcc cccgacgcgg ccaggctcca gcgcggaccc gtgccgggag
      721 aaagtacgga tatagcgcga tcaccgggta tcgtcggatt cgatcggcgt ggcgcgggtc
      781 gacgcctcgt cgtcgccgtc cgcgtgttcg cgcgggttac agtttcctcc tcgggtctga
      841 tataggggct cgccgggccg ctcgaggctt cggagatcca ccgccccacg gcgcacgtcc
      901 gagcgggccc gcggggatgc cgatgcgcaa ccgcaagaac tggagatgtc gctgatgtcg
      961 cagaaagatg ccgtatctat ccttggcctg ggtcagatgg gggccgcgct ggcgcgcgcc
     1021 tatatcgatg cgggtcatcg cgtgaccgtg tggaaccgat ctcccggcaa ggcagagccg
     1081 tttcacggtc aggcgaaggt ggcggagaca cccgcgcagg cctgcagcga gggcgatctg
     1141 acgatcgtct cggtgtcgaa ctaccaggcg agcgacgagg tcctccggac tcccctcgtc
     1201 gcgcaggcca tgaagggccg gacgatcgtt cagctgacga gcggcacgcc cggcgacgcc
     1261 cgcagcgggg cggcctgggc gtcggagcac gggatcgact acctggacgg ggccatcctg
     1321 gcatacccca gctacatcgg cggtgagacg accacggtct tctactcggg tcccagggcc
     1381 gtctacgatc gccacgaagc gacgctgcgc gtcatcggtg gcggcacctc gcatgtcggc
     1441 gagccgatcg gcgcggcggc gaccctcgat tgcgccctgt tggagagcta ctatggcgcc
     1501 accctctcgt ttttgcacgg cgcggcgatc tgcgcgtcgg agaagttccc cctggacgcc
     1561 tatttcgccg gcgttcaggc gatcatgccg ctcaacgcga tcaccgtcga catgtgcaaa
     1621 aagatgctgg cgagcggcga cttcaagggg accgactgca cactggatat ccacacgggg
     1681 gccatcgagc acatcgtgcg tctcagccag gagaacagcg tcgatcggcg cattccggag
     1741 ctcatcttgt cgtacttcaa gcgcgcgctg cagctcggtc acggcgcaga cgagatggcc
     1801 gccgtgttca acgccatcaa agacccgtcg cgcggctgat agcgacctcc cgctgtgcat
     1861 cccgtcgtgc cgtcgatcgg cgcatcgacg gcgaccgact ggcgcgtggg cccgggtcag
     1921 ggcacctcga tcaccaccgt cgcctccatg ctgtccgggt agacgagctc cgtcgcgaac
     1981 accgacaaca tctcgccggg ctcccctccg aacacccgac accagccgtt gaccggcggg
     2041 gcgtcgcact cgcacgcgcc gacgcacgtg ctaaacggga acgcaccgac ggcgtacttc
     2101 ccggccgcca gcggctcggc gcgcaggcac gcgccctgac agttggtccc gggcgcacac
     2161 tcggccgtca tctccatctc cacgtattgg atcgccggcc gagtgatcgt gatgctcgcg
     2221 cccgggtcga ggcgcagcaa cgtcggagcc gcgcaatctt ccccgccgac ctcacattcg
     2281 gacccggcgc tcagcagctc gtcgcacagc ttcgaactac cgtcgctcgt ggtctgcagc
     2341 agcggctgcc cgagcaggtt ggacagcagg atatgcggga gcccggcgca ggtctcggct
     2401 tcgatccaca ccggctcggt gcccgtcttg tactggaccg tcagctgcaa gctcggcccg
     2461 aagtccgccg aggacaggta ttcctcgcac gcggcagccc cggtggtcgg ctcggtcgcg
     2521 gtgccgccgg tcgtcggcgt cccggtcgtc gcgccggtct ccgtcccgct ctcgctctcc
     2581 ccgctgtcct cgctgccgcc cttgccgggc gaacacgctg ccagtaacgc cccaagaaga
     2641 actaaacgaa cagtgtcgcg aaacatgcgg cgcagcctac cccattcgcg ggtcggacgt
     2701 cggtcgtaat tccgcccgtc accgtcgcgc gcgaccattc gcccgcgagg attacagcag
     2761 caggccgggc agcgggccgc tgccggcctc gacgtcgtta tctccgtagt tatcgatgtg
     2821 cgtctggccc atcgcgtgga ggatgccgac cagcaggcgg tggtgggcga cgtcttcgtg
     2881 ggtggagaag cggccggtgc ggaggaactt cttgccgccg aggatgacga agggcatctg
     2941 tttaaaagag tggctaaagc cgttggcgac ctccgagccc cacacgatca tcgtattgtc
     3001 gagcatcgtg ccgtcgccct cggggaccgc ggcgagcgcg tcggccagct cgcggatctt
     3061 ctcggcgtag tagcgccgga tcttgacgag gaaggcctgg acctcgttgc cgggggcctc
     3121 gttgtggctg tgcgcgtggt ggaagtcggc gggtgggccg gagatccacg gataggtgtt
     3181 cgcgtcccac tcgccgcgcg cgacctgcag cgtgaccacg cgggtcaggt cgcagcgcag
     3241 ggccgcgacc gcgagctcga tgtgccgcga cagcacctcg ctgctcgcct cgttggcgtc
     3301 gaaggcctgc atcggcgcgg gggtctcggg cacctcgcag gtgtagccat tttgcagctt
     3361 ggtctcgatg tcctgcaggg cctgcaggtg cgagtcgatc ttcgggcgct cctcggcgcc
     3421 gatgcgggtc tgcaagccgg acagctcggc gcgcacgacg tcgatcgagc tcatgcgctt
     3481 ggttcgcagg cgctcgtagg cggccttgtc gagcgacgcc agcccgaaca gctgctgcca
     3541 ggcgacctgc gggtcctgaa acagctcggt cggggtgtcg ggcgcggtaa aggacatgcg
     3601 attggcgacg ctgaggccgc cggcttgata accgaggtgc agcgacggca gcgcggtcac
     3661 gccgagggtg ttcgcggcga tgacctgatc aatcgacgcg ttgacgttgc cgccccagcc
     3721 gagcgggtcg tccggcggcg cgccgccgtg cttgtacttg tcgcccggga gctgctcgga
     3781 gccggtgaac agcagcggca tgccgcgcgt gtgcgggccg gcgccgccct gaccgagcag
     3841 ctgcaggccc ttgaggacga tcaggttgtc cgcccggtac ggctcgaagg gcgcgagcag
     3901 ctcgggcagg gtgaaattgg tctcgctgcc ggtcggcgtc cacatgtcgt ggatcgttcc
     3961 atgcggcgtg tagacgagga tcagccgtcg cggcgggttg acgtcctcgc cgcgcgcgag
     4021 catcgggatg aacggcgcga gcgcggccga gaccccgagc gactgcagca ggcgccggcg
     4081 cccgaggcga ccgtcgaaaa agcgtggctg gacgatcttc atgggctctc cttcacgggg
     4141 tggcgaccac cagggtgcga aacgccgggc tcgtgacgat gtcggtcagc agcgcccgca
     4201 tgtcgccgtc gtggccggcg aagcgctgct ggaggtcgtc gacctggcag gtatcggtgg
     4261 tgctgaggat gcggccgagc gcgtaggtgg tccactggaa cgccacgcag tcgcgcacgg
     4321 agtcctcggt ggcgagcagc ttggcgagct cgaccggacc gacgaacggc tgctccccgg
     4381 tcccgtagct gcgcaacacg ccgctctcgt cgatcggctc gccgttctcg agctcgcgcc
     4441 acacaccgta ttgatcgtac ccctcgaagc cgaagccgat cccgtcgatg aaggtgtgac
     4501 acccctggca gctcggcacg ctggtatgca cctcgaagcg ctcgcgcgtg gtcgcgttcg
     4561 ggtccggcgg cggcggcttg atgtcgttcg gcggcgccgg caagtacgtg caaagcaggc
     4621 tctcgagcac caccacgccg cgcagcaccg gcgaggactg cccggggttg gcgtgcgtcg
     4681 tcagccagcc gacctgcgtc agcaagccgg cccggcgcgc cgggtcgagc tgcaccggcg
     4741 cgttgggctc ggccggcgcc tcgacgccgt agagcgggta cagctcgggc ggcaccagcg
     4801 agtagggcgc ggtcagcatc gtctcgacgc ggccgtcgtc gtcgcgcatg acggcggtca
     4861 tgaagtcgac cgcctcccgc ttcatcgccg cgcgcagctc cggggtcagc tcggggacca
     4921 gcggatgcgg ggacttctcg gtccagtcga ggtggtcgag ctcgagccac tgcaccacga
     4981 atgcaccgat gccgcggctg gcgcggtcgt cgtacaacat gcgctcgagc tgggccagca
     5041 gcaccgcgtc gtcggccagc gtcccggcgt cggcggccgc gcgcagcgcg tcgtcgggca
     5101 tgctgctcca caggaagtac gacagccggg ccgcgagctc gtggtcggtg agcgccgccc
     5161 agccgcgagc gtccggcgcg gcgcggccga gctcgacctg gtagaggaag tacggcgatt
     5221 gcaacatcgt cgcgatcacc tggcgcagcg cggtccccgg ggtctcctcg gtgagcgcga
     5281 gggtggtctc gtagaccccg agataacgcg cgaattcttc ctccgtgagc ggatggcgca
     5341 gggccctgct gccgaaggtg tcgatgaaat ctcgcacgca gctggccagc tcgggggcgt
     5401 cgggcgacag cgccgcggga tcgcagggga ccaggagggt gagatcggcg acgcccgcga
     5461 tctgctcggc gatgtcggtc aacgaggata cgccgatcac cgtcagcggc gcgccgatgt
     5521 tgctggtgaa ggtgccgacc ttggcgtcga gcccggcgag cgcgtccgtc ggcaccgagc
     5581 cgacctgcag caggtcctgg atcgtatagt catactgcgc gcgggtcagt cggcgcagcg
     5641 g
//
LOCUS       DASWNJ010001987         2731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_458236, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001987 DASWNJ010000000
VERSION     DASWNJ010001987.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458236"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..1380
                     /locus_tag="VGB85_33130"
     CDS             142..1380
                     /locus_tag="VGB85_33130"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY0138993.1"
                     /translation="MSKQEEDRWQPLWDDPDAAAQMLADPASLREFVAHAAELRRAHE
                     ELEMLHEATLEHASEIEDLLAVKIDEVEALVVDLELRNGFIREVFGRYITDDVVNTLL
                     ASPAGLQLGGEKRKITILISDVRGFSSLCERLTPEQVVAILNIYLGGMAEVIERFRGS
                     INEFIGDAILAVFGAPIAAEDTAERAVACALGMQLAMDEVNATLRAQGLPALEMGIGV
                     HTGEVVVGNIGSRKRAKYGVVGSHVNLTSRIETYTVGGQVLISESTAGELGDRLRTRR
                     SFQVQPKGARDALTIYDVVGITGTYELALPEVQAVRRALDPGVAAHFTVLEGKDASGE
                     AHPAILVSLDDHGEAELRGTPVPALLANIKFGLEGVAGEGAYAKVLDVSPEQGSFRVR
                     LAAVPPGLAAALDALPSRPG"
     gene            complement(1391..>2731)
                     /locus_tag="VGB85_33135"
     CDS             complement(1391..>2731)
                     /locus_tag="VGB85_33135"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY0138994.1"
                     /translation="GFAHARGVLHCDIKSANVMVGEFGQVYLMDWGGAQLLPPRPGVD
                     TRKWVRDSLPPLPPADTKGLVFGTPAYMSPEQASAGRDPMDERSDIFSMGALLYEIMT
                     GKPPFQAMSPMEGIMLAQICDIPAPDELPEVPPNTFPAELVRIVMKALAKRPDDRYRS
                     VAEMKLAILRLMRGGGNFATVRFSRGTHVIRQGEVGDAAYIVVSGKLEVYKTIDGERI
                     SLRQLGPGDMFGETAIFAASPRTASVVVTADASMVVVTRDVIEQELDSMKPWMGAFIR
                     TLATRFGGVEEERRARSHSGPSMSAVGPISPETADASLRISPLNQRPGRPVPASPAPT
                     STRPASGPTAAVFQDRSDITRVSDLASMGMPDPRREGPRAATGAIGEPVFGESVMIDL
                     SDVDDPVHARAETTLPAPTSRDAATTTVESIANEASEDDADTRLISADPRKSPR"
BASE COUNT          399 a          970 c          966 g          396 t
ORIGIN      
        1 cgctcgcagg ccgccgggcc ccacacctca cgaccacgcc atccgccagc cgggcaccac
       61 acctcccgac caccccatcc gccagccgag caccgcacct ctcgaccacg ccatccgagc
      121 gaatagcccc ccaggaacac catgagcaag caagaggaag atcgctggca gcccctgtgg
      181 gacgacccgg acgccgccgc ccagatgctg gcggatcccg cgagcctgcg agaattcgtg
      241 gcgcacgcgg ccgagctgcg tcgggcgcac gaggagctcg agatgttgca cgaggcgacg
      301 ctcgagcatg ccagtgagat cgaggatctg ctggccgtca agatcgacga ggtcgaggcg
      361 ctggtggtcg acctcgagct gcgcaacggc tttatccgcg aggtgttcgg gcgctacatc
      421 accgatgatg tcgtcaacac gttgctggcg tcgccggcgg gtttgcagct cggcggtgag
      481 aagcgcaaga tcacgatact gatctccgat gtacgcgggt tctcgtcgct gtgcgagcga
      541 ctgacgccgg agcaggtggt ggcgatcctc aacatttatc tcggggggat ggccgaggtc
      601 atcgagcgct ttcgcgggtc gatcaacgag ttcatcggcg acgcgatcct cgcggtgttc
      661 ggggcgccga tcgccgccga ggacaccgcg gagcgggcgg tcgcctgcgc gctggggatg
      721 cagctggcga tggacgaggt caacgcgacc ttgcgcgcgc agggcttgcc cgcgctcgag
      781 atggggatcg gcgtgcacac cggcgaggtc gtcgtcggca acatcggctc gcgcaagcgg
      841 gccaagtacg gggtcgtcgg cagccacgtc aacctgacca gccggatcga gacgtacacc
      901 gtcggcggcc aggtgttgat ctcggagtcg accgccggtg agctcggcga ccggctgcgc
      961 acgcgccgga gcttccaggt ccagcccaag ggcgcccgcg acgcgctgac gatctacgac
     1021 gtcgtcggca tcaccggcac ttacgagctc gcgctgcccg aggtccaggc cgtgcgtcgc
     1081 gcgctcgacc ccggcgtcgc cgcgcacttc accgtgctcg agggcaagga cgcctcgggc
     1141 gaggcccacc cggccatcct cgtcagcctc gacgaccacg gcgaggccga gctgcgcggc
     1201 acgccggtgc ccgcgctgct cgccaacatc aagttcgggc tcgagggtgt cgccggcgag
     1261 ggcgcgtacg ccaaggtgct cgacgtctcg cccgagcagg gcagctttcg cgtccgcctc
     1321 gccgcggtgc cacccggcct cgccgcggcg ctcgacgccc tgccctcgcg gcctggctga
     1381 tcgcgccgct tcatcgcggc gacttgcgcg gatcggcgga gatcagacgc gtgtcggcgt
     1441 cgtcctcgct ggcctcattc gctatcgact cgacggtggt cgtcgcggcg tctcgggacg
     1501 tgggcgccgg cagcgtcgtc tcggcgcgcg cgtgcacggg gtcgtcgacg tcggagaggt
     1561 cgatcatcac gctctcgccg aacaccggct ccccgatcgc gccggtggcc gcgcgcgggc
     1621 cctcgcgccg cggatccggc atccccatgc tggcgaggtc ggacacccgc gtgatgtcgg
     1681 acctgtcctg aaagacagcc gccgtcggac ccgacgccgg cctcgtcgac gtcggcgcgg
     1741 gcgaggcagg taccggccgg ccgggacgct ggttgagcgg cgagatgcgc agcgaggcgt
     1801 cggccgtctc gggcgagatc gggccgaccg ccgacatcga cgggcccgag tggctgcgag
     1861 cgcggcgctc ctcctcgacg ccgccgaagc gggtcgccag cgtgcggatg aacgcgccca
     1921 tccacggctt catcgagtcg agctcttgct cgatgacgtc gcgggtgacg accaccatcg
     1981 acgcgtccgc ggtgacgacg acgctggcgg tgcgcggcga cgcggcgaag atcgccgtct
     2041 cgccgaacat gtcgccgggg ccgagctggc gcagcgatat gcgctcgccg tcgatcgtct
     2101 tgtagacctc gagcttgccg gacacgacga tgtacgccgc gtcgccgacc tcgccctggc
     2161 ggatgacgtg ggtgccgcgg gagaagcgga cggtcgcgaa gttgccgccg ccccgcatca
     2221 gccgcaagat cgccagcttc atctcggcga cgctgcggta gcggtcgtcg gggcgcttgg
     2281 cgagcgcctt catgacgatg cgcaccagct ccgccgggaa ggtgttcggc ggcacctcgg
     2341 gcagctcgtc gggcgccggg atgtcgcaga tctgcgccag catgatcccc tccatcgggc
     2401 tcatcgcctg gaagggcggc tttccggtca tgatctcgta cagcagcgcg cccatcgaga
     2461 agatgtcgct gcgctcgtcc atcgggtcgc ggccggcgct ggcctgctcc ggggacatgt
     2521 acgccggcgt gccgaacacc aggcccttcg tgtccgccgg cggcagcggc ggcagcgagt
     2581 cacgcaccca cttgcgcgtg tcgaccccgg ggcgcggcgg cagcagctgc gcgccgcccc
     2641 agtccatcag gtagacctgc ccgaactcgc cgaccatcac gttggccgac ttgatgtcgc
     2701 agtgcaggac cccgcgcgcg tgggcgaagc c
//
LOCUS       DASWNJ010001988         3662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_140442, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001988 DASWNJ010000000
VERSION     DASWNJ010001988.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140442"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            524..1120
                     /locus_tag="VGB85_33140"
     CDS             524..1120
                     /locus_tag="VGB85_33140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138995.1"
                     /translation="MSQPTSDPSPQQRAPPGPAAAVDLDSSPVVRASFSMRGFMDGWM
                     HCRHVADYLARFAASDRYDPERLTTRLSNYLNEALELVFRSAPGPGQLIVAIYRQHDR
                     LEVTLACPVTADDRMRRSVRRATEAEAAANYRRGFVDLVRPGDGGRAPPASEDPDEWE
                     ADAGLLELVALHGITLALSSVADDVDNAVTLHMTVPHE"
     gene            1132..2310
                     /locus_tag="VGB85_33145"
     CDS             1132..2310
                     /locus_tag="VGB85_33145"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0138996.1"
                     /translation="MDPAQPYNVHYHENDQEVAIAGSLRPRDAADFEPVRACLVRVAA
                     SIKGTLYINLKRLRYCNHRAFLELARFLEHCRREHPDLRLKLVISSVVPWAPIRFAYL
                     TELFPNAIVEQYDRAFYPGQGVIENDQLIPVLRAQTNIVWEHERELLRRHGLRHGMRV
                     ADICCGIGDFAVLLHKEFKPEVIVAVDHSRPFLQYAIQVAKEFGINDIEYQYGDAADL
                     FLPANTFDFVTSRLSLQIFDQPGLILQELLRICKPGGRVYLTNEMMSHNYGYPRHESV
                     SWTYAQTMKMFATLGMDLSIGPRMFSLLTDMGFEDVQMEQIGITNANTLVEDFARVIE
                     SWEEYITGELSTATGQPRDIVERLKAGFQDHVYAIRSRRGFAIWPIFVASGRKPVTTR
                     "
     gene            2406..2990
                     /locus_tag="VGB85_33150"
     CDS             2406..2990
                     /locus_tag="VGB85_33150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138997.1"
                     /translation="MNECIDCIGDFRDDWPSTEGGGVRVSVVFYPADFAVRWSQCSAT
                     ADFFAEYFAAIDACRSDDPAARDEFIGTLSYIVNELVENAVKFSVGETVEVTVGVEDG
                     ELVVMAANQILASTAPPLIEKFRELLAGDPHELLFARVEANAEDPEAGASGLGFLTMM
                     ADYAARLGWRFAAVHDNPHNVLLTTMARLEVRKP"
     gene            2987..3337
                     /locus_tag="VGB85_33155"
     CDS             2987..3337
                     /locus_tag="VGB85_33155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138998.1"
                     /translation="MSESEIKGPGYRVWYDAEATTVHLEGSLRLGTTEYEPIAALLNS
                     VIERHPPAITLRMTDLVFLNSSGINTLYKFAIAIRKLGDIAVHVHASSTISWQSKSLP
                     NLRKFLPGITLTMN"
     gene            3351..>3662
                     /locus_tag="VGB85_33160"
     CDS             3351..>3662
                     /locus_tag="VGB85_33160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0138999.1"
                     /translation="MTWIVERCLVAGHLLACSGDAMLGCLVARSNSAGLGCLVACSGD
                     AMLGCLVACSGDAMLVACPDSAGRGCLGRGAGAGGLRVPATRVLPGHVLAAPPVRKNM
                     SL"
BASE COUNT          571 a         1382 c         1139 g          570 t
ORIGIN      
        1 gacagcaacc gctcgttcgg cacgtcctcg tcgacctcct cgagcagttg cccggcgatc
       61 acgggaccca cgagctgttc ccggtggcgg cgaaggtcct gacacgccga caggatgatc
      121 ttgtagcgat cctcgaaaat catgtcgctg tggccaccca ccgccccgcc tcggtgccgc
      181 tcaaggtcac acaccgcgcc gagtcttggc aaggtcggca tgcacatgtc ccaagagaca
      241 ggagaggcat cgaagccccg cagcgacccc ccatcctcgc gtggaatcga gcgcgcgcga
      301 acccgcccgc gcccggctcg ccgcgcccga cctttcgccg gaagcgaccc cgtccgccgc
      361 ttctggcccg gtccgaaccc ccgcccggtc ggccctcccg gccgtcgcga cccggtcgga
      421 cttcccggcc gttgcggccc ggtttgactt cccggccgtt ccggcctgaa ggtccttgct
      481 ccccgcgctc accggtgggc actctaacac cctggcaccg atcatgtcgc agcccacctc
      541 cgacccgtcc ccgcagcagc gcgcgccccc cgggcccgcg gcggcggtcg acctggactc
      601 gtcgccggtg gtccgcgcga gcttctcgat gcggggcttc atggacggct ggatgcactg
      661 ccgccatgtc gcggattacc tggcccgctt cgccgcctcg gaccgctacg atccggagcg
      721 cctgacgacg cgcctgtcga actacctcaa cgaggcgctc gagctggtgt tccgcagcgc
      781 gccgggcccc ggccagctca tcgtagcgat ctaccgccag cacgaccgcc tggaggtcac
      841 cctcgcctgc cccgtgaccg ccgacgaccg catgcgccgc agcgtccgcc gcgcgaccga
      901 ggccgaggcc gccgcgaact accggcgagg cttcgtggat ctcgtgcgcc ccggcgacgg
      961 cgggcgcgcc cctccggcaa gcgaggaccc cgacgagtgg gaggccgacg ctggcctgct
     1021 cgagctggtc gccctgcacg gcatcacgct cgcgctgtcg agcgtcgcgg atgatgtaga
     1081 taacgcggtc acgctccaca tgaccgtccc gcacgaatga cgaggccgcc gatggatccc
     1141 gcgcagcctt acaacgtcca ctatcacgag aacgaccagg aggtcgcgat cgccggctcg
     1201 ctgcgcccgc gcgacgccgc ggacttcgag cccgtgcggg cgtgcctggt gcgcgtggcg
     1261 gcgtcgatca agggcacgct gtacatcaac ctcaagcgcc tgcgctactg caaccaccgg
     1321 gcgttcctcg agctggcgcg cttcctcgag cactgccgcc gcgagcaccc cgacctgcgc
     1381 ctcaagctgg tgatctcgag cgtggtcccg tgggccccga tccgcttcgc ctacctgacg
     1441 gagctgttcc ccaacgcgat cgtcgagcag tacgaccggg cgttttaccc cggtcagggc
     1501 gtgatcgaga acgaccagct gatccccgtc ctgcgcgccc agaccaacat cgtctgggag
     1561 cacgaacgcg agctgctgcg ccgccatggc ctgcgccacg gcatgcgcgt cgccgacatt
     1621 tgctgcggga tcggcgactt cgccgtgctg ctgcacaagg agttcaagcc cgaggtcatc
     1681 gtcgccgtcg accactcccg gcccttcctc caatacgcga tccaggtcgc caaggagttc
     1741 gggatcaacg acatcgagta ccagtacggc gacgccgccg acctgttcct ccccgccaac
     1801 accttcgact tcgtcacctc gcgcctgtcg ctgcagatct tcgaccagcc gggcctcatc
     1861 ttgcaggagc tgctgcggat ctgtaagccc ggcggccgcg tctacctgac caacgagatg
     1921 atgagccaca actacggcta tccgcgccac gagtcggtca gctggaccta cgcgcagacg
     1981 atgaagatgt tcgccaccct cggcatggac ctcagcatcg gccccaggat gttctcgctg
     2041 ctgaccgaca tgggcttcga ggacgtccag atggagcaga tcggcatcac caacgccaac
     2101 acgctcgtcg aggacttcgc gcgcgtgatc gagtcgtggg aggagtacat caccggcgag
     2161 ctgtcgaccg cgaccggcca gccgcgtgac atcgtcgagc gcctcaaggc cggcttccag
     2221 gaccacgtct atgcgatccg cagccgccgg ggcttcgcga tctggccgat cttcgtcgcc
     2281 tccggccgca agccggtcac cacccgctga aacacctcca ccccgcacat gcgcccccac
     2341 ccgccccagc gccgcgccga cctgtgttac cgtcagcggc gtagccgagc tccgagacga
     2401 ccgccatgaa cgagtgcatc gattgcatcg gggacttcag ggacgactgg ccgtcgaccg
     2461 agggcggcgg cgtgcgcgtg tccgtcgtct tctacccggc ggacttcgcc gtgcggtgga
     2521 gccagtgcag cgccaccgcc gacttcttcg ccgagtactt cgccgccatc gacgcctgcc
     2581 gcagcgacga ccccgccgcc cgcgacgagt tcatcggcac gctgagctac atcgtcaacg
     2641 agctcgtcga gaacgcggtc aagttcagcg tcggcgagac cgtcgaggtc accgtcggcg
     2701 tcgaggacgg tgagctcgtc gtgatggccg ccaaccagat cctcgcgtcg accgccccgc
     2761 cgctgatcga gaagttccgc gagctgctcg ccggcgaccc ccacgagctg ttgttcgccc
     2821 gcgtggaggc gaacgcggag gatcccgagg ccggcgcctc gggcctcggg tttttgacga
     2881 tgatggcgga ctacgccgcc cgcctgggct ggcgcttcgc cgccgtccac gacaaccccc
     2941 acaacgtgct gctcacgacg atggcacggc tagaggtcag gaagccatga gcgaatcgga
     3001 gatcaaaggc cccggctacc gagtgtggta cgacgcggag gccaccaccg tgcacctcga
     3061 gggctcgctg cgcctcggca ccaccgagta cgagccgatc gccgccctgc tcaactcggt
     3121 gatcgaacgc caccccccgg cgatcaccct gcgcatgacg gacctcgtgt tcctcaacag
     3181 ctccggcatc aacacgctct acaagttcgc catcgcgatc cgcaagctcg gcgacatcgc
     3241 cgtccacgtc cacgcctcct cgacgatctc gtggcagagc aagtccttgc ccaacctgcg
     3301 caaattcttg ccgggtatca cgctgaccat gaactgatcc cccgggctcg atgacctgga
     3361 tcgtagagcg ctgcctcgtc gccggtcacc tgctggcgtg ctcgggtgac gcgatgctcg
     3421 gctgcctcgt cgcgcgctcg aacagcgcgg gactcggctg cctcgtcgcg tgctcgggtg
     3481 acgcgatgct cggctgcctc gtcgcgtgct cgggtgacgc gatgctcgtc gcgtgcccgg
     3541 acagcgcggg gcgaggctgt ttgggccggg gagcgggggc cggcgggctg cgggtccctg
     3601 cgacgcgggt cctcccaggg cacgtgctcg ccgccccacc tgtccggaag aacatgtcac
     3661 tt
//
LOCUS       DASWNJ010001989         2614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_418528, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001989 DASWNJ010000000
VERSION     DASWNJ010001989.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418528"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1385
                     /locus_tag="VGB85_33165"
     CDS             <1..1385
                     /locus_tag="VGB85_33165"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEY0139000.1"
                     /translation="LVSWQREQLSVRDDHVSDVGLRTDYGLGVRVLADGAWGFAATQK
                     LDSKSVTDAARSAVAMAKRSALLIPEPITLVPTPVVRGAWVSPMQVDPFAVPMADKAA
                     LLRAAARAAMDVKGVAHVDATLLSVREEKLLITSEGSRVHQIFHRVMPGLTATAIDRR
                     RGRFASRDHEVAPMLAGYEHVTAADLVGQAPVIAEEALQKLHAAPVEPGKKQVILAPS
                     NLWLTIHESIGHSTELDRALGWEANFAGTSFLTPDKRGKLKIGSDRVQIIADRTQPGG
                     LATTAWDDEAVPAQRWPIVQDGVFVGWQTTRDLASKVGDTSSRGCLYADGYGSVAFPR
                     MPNVSLQPGLEGHTTEDLINATDDGILISGRGSWSIDQQRYNFQFGGQFFWEIKKGRL
                     TRPLRDVAYQANTVEFWNSCDMLGGDASYRLGGSFSDGKGEPSQSNAVSHGCPPARFT
                     VNVINTGSGT"
     gene            1382..>2614
                     /locus_tag="VGB85_33170"
     CDS             1382..>2614
                     /locus_tag="VGB85_33170"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEY0139001.1"
                     /translation="MTLRRNEAQALVKAAIKAATLKDIRVRVGSSRSGNLRFAGNAPT
                     TSGEVDRLDVSVTATRDGASATVSGAVSDGAGVAALVGQAEALAALAPKDPEHMPPLG
                     PQTYLRVDAEDPAAVKLGAEQRTEAVARVLKVARDGKLVASGLYEHVHRSMALGTSAG
                     LFAYHAETAVSLTTTCRTPDGSGSGWGGAMSHRLADLDATAVARRAADKAEMTRGAAP
                     ALTPGAYTVVLEAQAVADLLGFLIGSLGARAADEGRSFFARPGGNAVGEQLFGPQIRV
                     YSDPQDPAHPSTPVGNDGLPLQKTTWIDQGKLVTLTSPRWWAQKQGRVAVPAPRSVFM
                     AGGTGDVATLVRGVQSGVLVTRLWYNRMLDPRQILATGLTRDGTFKIDGGKLAGAVKN
                     LRDNDSPVTLLKQALALGK"
BASE COUNT          408 a          954 c          924 g          328 t
ORIGIN      
        1 ggctggtctc gtggcagcgc gagcagctga gcgtgcgcga cgaccacgtc agcgacgtcg
       61 gcctgcgcac cgactacggc ctcggcgtgc gcgtgctggc ggacggcgcg tggggcttcg
      121 cggcgacgca gaagctcgac agcaagtcgg tcacggacgc ggcccgcagc gcggtggcga
      181 tggcgaagcg cagcgccctg ctgatccccg agccgatcac gctcgtgccg acgccggtgg
      241 tccgcggcgc ctgggtctcg ccgatgcagg tcgacccctt cgcggtgccg atggccgaca
      301 aggccgcgct gctgcgcgcg gcggcccgcg ccgcgatgga cgtcaagggc gtcgcgcacg
      361 tcgacgcgac cctgctgtcg gtccgcgagg agaagctgct gatcaccagc gagggctcgc
      421 gcgtgcatca gatcttccat cgcgtgatgc cgggcctgac ggcgaccgcg atcgaccgcc
      481 gccgcggtcg cttcgccagc cgcgaccacg aggtcgcgcc gatgctcgcc ggctacgagc
      541 acgtcacggc agcggacctc gtcggccagg cgccggtgat cgccgaggag gcgctacaaa
      601 agctgcacgc cgcgccggtc gagccgggca aaaagcaggt gatcctcgcg ccgagcaacc
      661 tctggctgac gatccacgag agcatcggcc actcgaccga gctcgaccgc gcgctcggct
      721 gggaggccaa cttcgcgggc accagcttct tgacccccga caagcgcggc aagctcaaga
      781 tcggcagcga ccgcgtgcag atcatcgccg accgcacgca gcccggcggc ctggcgacga
      841 ccgcgtggga cgacgaggcc gtgccggcgc agcgctggcc gatcgtgcaa gacggcgtgt
      901 tcgtcggctg gcagacgacc cgcgacctcg cgagcaaggt cggcgacacc agcagccgcg
      961 gctgcctgta cgccgacggc tacggcagcg tcgccttccc gcggatgccc aacgtcagcc
     1021 tgcaaccggg cctcgagggc cacaccacgg aggacctgat caacgcgacc gacgacggga
     1081 tcctgatcag cggccgcggc agctggtcga tcgatcagca gcgctacaac ttccagttcg
     1141 gcgggcagtt cttctgggaa attaaaaagg gccggctgac ccggccgctg cgcgacgtcg
     1201 cctaccaggc caacaccgtc gagttctgga actcgtgcga catgctcggc ggcgacgcca
     1261 gctaccgcct cggcgggtcg tttagcgacg gcaagggcga gccctcgcag agtaacgcgg
     1321 tcagccacgg gtgcccaccc gcgcgcttca ccgtcaatgt gatcaacacc gggagcggca
     1381 catgacgctg cggcgaaacg aggcccaggc gctggtcaag gccgccatca aggccgcgac
     1441 cctcaaggac atccgcgtgc gcgtcggcag cagccgcagc ggcaacctgc gcttcgccgg
     1501 caacgcgccg acgacctcgg gtgaggtcga ccgcctcgac gtcagcgtca ccgcgacgcg
     1561 cgacggggcc agcgcgaccg tctccggcgc cgtgagcgac ggggcagggg tcgcggcgct
     1621 cgtcggccag gccgaggccc tcgccgcgct cgcgccgaag gaccccgagc acatgccgcc
     1681 gctcggcccg cagacctacc tgcgcgtcga cgccgaggac cccgcggcgg tcaagctcgg
     1741 cgccgagcaa cggaccgagg cggtcgcgcg ggtcctcaag gtcgcgcgcg acggcaagct
     1801 ggtcgcctcc ggtctttatg aacatgtcca tcggagcatg gccctcggga catccgccgg
     1861 gctgttcgcc taccacgccg agacggcggt ctcgctgacg acgacctgtc gcacgccgga
     1921 cggcagcggc agcgggtggg gcggggcgat gtcgcatcgc ctcgccgacc tcgacgcgac
     1981 cgcggtcgcg cggcgagccg cggacaaggc cgagatgacc cgcggggccg cgcctgcgct
     2041 gacgccgggc gcctacacgg tggtgctcga ggcccaggcg gtcgccgacc tgctcggctt
     2101 cttgatcggc tcgctcggcg cgcgcgcggc cgacgagggt cgcagcttct tcgcgcgtcc
     2161 cggcggcaac gcggtcggcg agcaattgtt cggcccgcag atccgcgtgt actccgaccc
     2221 gcaggacccc gcgcacccgt cgacgccggt cggcaacgac ggcctgccgc tgcaaaagac
     2281 cacgtggatc gatcagggca agctggtcac tctgacttcg ccgcgctggt gggcgcaaaa
     2341 gcagggccgc gtcgccgtgc ccgcgccgcg ctcggtgttc atggccggcg gcaccggcga
     2401 cgtcgccacg ctcgtacgtg gtgtgcagtc cggggtgctc gtcacgcggc tctggtacaa
     2461 ccgcatgctc gacccgcggc agatcctcgc caccggcctg acgcgcgacg gcacttttaa
     2521 aattgacggt ggcaagctcg ccggggcggt caagaacctg cgcgacaacg acagcccggt
     2581 gaccctgctc aagcaggcgc tcgcgctcgg caag
//
LOCUS       DASWNJ010001990         2599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_458484, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001990 DASWNJ010000000
VERSION     DASWNJ010001990.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458484"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /gene="ribD"
                     /locus_tag="VGB85_33175"
     CDS             complement(<1..1158)
                     /gene="ribD"
                     /locus_tag="VGB85_33175"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEY0139002.1"
                     /translation="MRPELAELLALIAGPGAMDMSSGTGSRATAEDTTAPAMRAALAV
                     VAAVGESAEERDRRFMGAAVQLACAGTGATYPNPCVGAVVVQAGRVVGAGHSAVTGGP
                     HAEVRALAMAGAAARGATVYVTLEPCSHHGRTPPCTDALIAAGVAEVAYGVQDPARHA
                     AGKGSALLRAAGIGVREGVELTACASAHEHYLHHERSRRPFVTLKAATSLDGRIAAAS
                     GDSKWITGEPARRVGHWLRARHHAIAVGADTLLRDDPRLDVRLVRGVDPVPVVVDSRL
                     RTAVADPRPQLLRPGTIVLHTEHAPEDRRRSLADAGVRLIAVAADERGRVTIAASPSA
                     SKPAGVHDIDLAPSALDALGRLPIRSLLVEGGGHLIASFVAAAAWDRWYWFQ"
     gene            complement(1148..1768)
                     /gene="nrdR"
                     /locus_tag="VGB85_33180"
     CDS             complement(1148..1768)
                     /gene="nrdR"
                     /locus_tag="VGB85_33180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEY0139003.1"
                     /translation="MLCPVCRGANIKVLDSRDSRDGVAIRRRRACSDCAHRFTTYERI
                     ETSLPMVLKRSGGKQAFDRNKILAGLKLAVRKRPVEDEQLENIVASVELWAATRGERE
                     IHAEEIGERIMQHLHALDPVAYVRFVSVYRSFDSVQAFAHLLHEREKAAQTSPEGQRT
                     LFEIDARGHIQLTPRSEAELDATTSGETGAVATDGEEPGGEPGDAT"
     gene            complement(1770..2243)
                     /gene="rpiB"
                     /locus_tag="VGB85_33185"
     CDS             complement(1770..2243)
                     /gene="rpiB"
                     /locus_tag="VGB85_33185"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEY0139004.1"
                     /translation="MSNEPTHAARARIHFGSDHAAIDLRLELAEAARTDGHVIASDAG
                     PVNAETSIDYPDVAAQVCERVLADPGSIGVLVCGTGQGMAMTANKMQGIRAAVVLDDF
                     SARMAREHNDANVLCLGARVAGSGLARTLLQTFLGATFAGGRHARRVAKIDAAGG"
     gene            complement(2246..>2599)
                     /locus_tag="VGB85_33190"
     CDS             complement(2246..>2599)
                     /locus_tag="VGB85_33190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HEY0139005.1"
                     /translation="QIGYINAHGTSTTADAIETQAIRAVFGAHADKLAVSSTKSMHGH
                     LLGAAGGLEAAISVLALRHGILPPTINLDDPDPACDLDYVPHTARHVQCEYVMSNSFG
                     FGGTNAALIFKRAEA"
BASE COUNT          314 a          892 c         1032 g          361 t
ORIGIN      
        1 ctggaaccag taccagcgat cccaggccgc ggcggcgacg aagctggcga tcaggtggcc
       61 gccgccctcg acgagcagcg agcggatcgg cagtcgcccg agggcgtcga gcgcggacgg
      121 ggccaggtcg atgtcgtgca caccggcggg cttcgacgcg gaggggctcg ctgcaatggt
      181 cacgcggccg cgctcgtcgg ccgcgacggc gatcaggcgc acgccggcgt cggcgaggct
      241 gcggcgccgg tcctccgggg catgttcggt gtgcagcacg atcgtgccgg ggcgcagcag
      301 ctgcggccgc gggtcggcga ccgcggtgcg cagccggctg tcgacgacca cggggacggg
      361 gtcgacgccg cggacgaggc ggacatcgag gcgcgggtcg tcgcgcagca gggtgtccgc
      421 gccgacggcg atcgcgtggt gacgcgcgcg cagccagtgg ccgacgcgac gcgcgggctc
      481 gccggtgatc cacttgctgt cgccgctggc ggcggcgatc cggccgtcga gcgaggtcgc
      541 ggccttgagc gtgacgaacg gccggcggct gcgctcgtgg tgcaggtaat gctcgtgcgc
      601 cgaggcgcag gcggtcagct cgacgccctc gcgcacgccg atgccggcgg cgcgcagcag
      661 ggcgctgccc ttgcccgcgg cgtggcgggc cgggtcctgc acgccgtacg cgacctcggc
      721 gacgccggcg gcgatcagcg cgtcggtgca cggcggcgtg cgcccgtggt gcgagcaggg
      781 ctcgagcgtg acgtagacgg tcgccccgcg ggcggccgcg ccggccatcg ccagcgcgcg
      841 gacctccgcg tgcgggcccc cggtgaccgc gctgtggccg gcgccgacca ccctgcccgc
      901 ctgcacgacg acggcgccga cgcaggggtt ggggtaggtc gcgccggtgc cggcgcaggc
      961 gagctgcacg gcggcaccca tgaacctgcg gtcgcgctcc tcggccgact cgccgacggc
     1021 ggcgacgacc gcgagggccg cgcgcatggc cggcgcggtg gtgtcctcgg cggtcgccct
     1081 ggaccctgtc ccggaggaca tgtccatcgc gccaggcccc gcgatcaggg cgagcagctc
     1141 ggccagctca ggtcgcatcg ccgggctctc cgccgggctc ctcgccgtcg gtcgcgaccg
     1201 cgccagtctc tccggaggtg gtggcgtcga gctcggcctc ggagcgcggg gtcagctgga
     1261 tgtggccccg ggcgtcgatc tcgaacagcg tgcgctggcc ctcggggctg gtctgggcgg
     1321 ccttctccct ctcgtgcagc aggtgcgcga aggcctgcac cgagtcgaag ctgcggtaca
     1381 ccgagacgaa gcggacgtag gcgacggggt cgagcgcgtg caggtgctgc atgatgcgct
     1441 cgccgatctc ctcggcgtgg atctcccgct cgccgcgggt ggcggcccac agctcgacgg
     1501 aggcgacgat gttctcgagc tgctcgtcct cgaccgggcg cttgcgcacc gcgagcttga
     1561 ggccggcgag gatcttgttg cgatcgaagg cctgcttgcc gccgctgcgc ttgagcacca
     1621 tcggcaggct ggtctcgatc cgctcgtagg tcgtaaagcg gtgggcgcag tccgagcacg
     1681 cgcggcggcg ccggatcgcc accccgtcgc ggctgtcgcg ggagtcgagc accttgatgt
     1741 tcgcgccgcg gcagaccgga cagagcaagc tagcctcctg cggcgtcgat cttggcgacg
     1801 cgtcgcgcgt gccggccacc ggcgaaggtc gcccccagga aggtctggag cagcgtgcgc
     1861 gcgaggccgg agccggccac gcgggcgccg aggcacagca cgttggcgtc gttgtgctcg
     1921 cgggccatgc gggcgctgaa gtcgtcgagc acgacggcgg cgcggatccc ctgcatcttg
     1981 ttggcggtca tcgccatgcc ttggcccgtg ccacagacga ggacgccgat cgacccggga
     2041 tcggcgagca cgcgctcgca cacctgggcg gcgacgtcgg ggtagtcgat cgaggtctcg
     2101 gcgttgaccg ggcccgcgtc gctggcgatc acgtggccgt cggtgcgcgc ggcctcggcg
     2161 agctcgagcc gcaggtcgat cgccgcgtgg tcgctgccga agtggatgcg cgcgcgcgcg
     2221 gcatgagtgg gttcgttgct catgatcagg cctccgcccg cttgaagatc agcgccgcgt
     2281 tggtgccgcc gaagccgaag ctgttcgaca tgacgtactc gcattgcacg tggcgggcgg
     2341 tgtgcggcac gtagtcgagg tcgcaggcgg gatcggggtc gtcgaggttg atcgtcggcg
     2401 gcaagatgcc gtggcgcagc gccagcaccg agatcgcggc ctcgaggccg ccggcggccc
     2461 cgagcaggtg gccgtgcatc gacttggtcg aggacaccgc cagcttgtcc gcgtgcgcgc
     2521 cgaacacggc gcggatcgcc tgggtctcga tcgcgtcggc ggtggtcgag gtgccgtggg
     2581 cgttgatgta gccgatctg
//
LOCUS       DASWNJ010001991         2609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_458512, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001991 DASWNJ010000000
VERSION     DASWNJ010001991.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458512"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2543)
                     /locus_tag="VGB85_33195"
     CDS             complement(<1..2543)
                     /locus_tag="VGB85_33195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0139006.1"
                     /translation="MASAAVLVDGVESHDVSVWSGHTGQLSEAVNAAVGNAATDFVVL
                     ADAGSLLAPDCLYQVALTSHRDPLVDLVVWDDDRLVDGARTDPRFRPSWSPDLLTGAD
                     YVGLSFAIRRSRLLFVHGVRDGFGDAMTWDLLLRADLSDERVARVPRVLTSVSRRPDD
                     VGPEGVRAVQEHLDRAGLAATAVAAGQGVRTQWTPDGDWPRVTVVIPTRHNRSMLSMC
                     LPSLATTDYPGGFDVVIVDNGGQSDDNDRWYAEHRAGLDLTVSWWTAPFNYSAVNNLG
                     ATLGDGEVLVFLNDDTEVLDPSWMTEIVGWARRPEIGVAGLQLIGPDGELQHAGAVLG
                     LGGFADHVFEGMAPGSPSLLGNAGWYRNVLAVTGACLAVRREVYDELGGFDERFLLTG
                     SDVAIGLDAKLKGYRNVCSPYAALRHLESATRGTHVPLEDFFTSYWRYNPWLFGGDPY
                     YSPNLSLGSRIPRLKGPHEPTPGERISGPLGRPFNVFRQKNDEAESRMLADNCRALPI
                     DQKAVDALHAANAEPFEVKTVNWFIPDIDSPFYGGINTALRIADQLAREHGVENRFVV
                     WGSPPDHFVRSALAAAFPSLADAPISFYDGSMGASLESVPEADVAIATLWVTAYAVAH
                     FPHAKRKFYLVQDFEPMFYPASTLYALTEESYKLGLYGLCNTDNLRQIYEGDYAGKGM
                     SFTPAVDPTVFHARGRVERMPDDPATVFVYARPGHWRNCWELASLALEELKDKLGDRV
                     RIVTAGSWAVPEGGFGGMHHYGLLDYRATGELYRTCDVGMALTVSKHPSYLPLELMAC
                     GVPVVAFDNPWGHWVLEDGTNSLLAKRTVDGLVDSLERAVVDYELRQRLR"
BASE COUNT          372 a          869 c         1011 g          357 t
ORIGIN      
        1 cgcaggcgct gccgcagctc gtagtcgacg accgcgcgct cgaggctgtc gacgagcccg
       61 tcgacggtcc gcttggcgag caggctgttc gtgccgtcct cgagcaccca gtggccccag
      121 gggttgtcga aggccaccac cggcacgccg cacgccatga gctcgagcgg caggtacgac
      181 gggtgcttgc tgacggtcag cgccatgccg acgtcgcagg tgcggtacag ctcgccggtc
      241 gcgcggtagt cgagcaggcc gtagtggtgc atgccgccga agccgccctc gggcaccgcc
      301 cacgagccgg ccgtgacgat ccgcacccgg tcgccgagct tgtccttgag ctcctcgagc
      361 gcgagcgacg ccagctccca gcagttgcgc cagtggccgg ggcgggcgta gacgaacacg
      421 gtcgccgggt cgtcgggcat gcgctcgacc cggccccggg cgtggaagac cgtcgggtcg
      481 acggccgggg tgaaggacat gcccttgccg gcgtagtcgc cctcgtagat ctggcgcagg
      541 ttgtcggtgt tgcagagccc gtacagcccc agcttgtagc tctcctcggt gagcgcgtac
      601 agcgtgctcg ccgggtagaa catcggctcg aagtcctgca ccaggtagaa cttccgcttc
      661 gcgtgcggga agtgcgcgac ggcgtacgcc gtgacccaca gcgtcgcgat cgccacgtcg
      721 gcctccggca cggactcgag cgaggcgccc atcgagccgt cgtagaacga gatcggcgcg
      781 tcggcgagcg acgggaaagc cgcggcgagc gcggagcgca cgaagtggtc gggcgggctc
      841 ccccagacga cgaaccggtt ctcgacgccg tgctcgcggg cgagctggtc ggcgatgcgc
      901 agcgcggtgt tgatgccgcc gtagaacggg ctgtcgatgt cggggatgaa ccagttgacg
      961 gtcttgacct cgaacggctc ggcgttcgcg gcgtgcagcg cgtcgacggc cttctggtcg
     1021 atgggcagcg cgcggcagtt gtcggcgagc atccgtgact cggcctcgtc gttcttctgc
     1081 cggaagacgt tgaacggccg gccgagcggg ccgctgatgc gctcgccggg cgtcggctcg
     1141 tgcgggccct tgagccgcgg gatgcggctg ccgagcgaga ggttcggcga gtagtagggg
     1201 tcgccgccga acagccacgg gttgtagcgc cagtaggacg tgaagaagtc ctcgagcggg
     1261 acgtgcgtgc cgcgggtcgc cgactcgagg tggcgcagcg cggcgtacgg cgagcagacg
     1321 ttgcggtagc ccttgagctt ggcgtcgagg ccgatcgcga cgtcgctgcc ggtgagcagg
     1381 aagcgctcgt cgaagccgcc gagctcgtcg tagacctcgc gccggacggc gaggcacgcg
     1441 ccggtgacgg cgaggacgtt gcggtaccag ccggcgttgc cgagcaggct cggcgagccg
     1501 ggcgccatgc cctcgaagac gtggtcggcg aagccgccga gccccaggac cgcgccggcg
     1561 tgctggagct cgccgtccgg gccgatgagc tgcaggcccg cgacgccgat ctcgggacgc
     1621 cgggcccagc cgacgatctc ggtcatccag gacgggtcga gcacctcggt gtcgtcgttg
     1681 aggaagacga gcacctcgcc gtcgccgagc gtcgcgccga ggttgttcac ggccgagtag
     1741 ttgaacggcg cggtccacca cgacacggtg agatcgaggc cggcgcggtg ctcggcgtac
     1801 cagcggtcgt tgtcgtcgct ctggccgccg ttgtcgacga tgacgacgtc gaagccgccc
     1861 gggtagtcgg tcgtggccag gctcggcagg cacatcgaca gcatcgagcg gttgtgccgg
     1921 gtcgggatga cgacggtgac ccgcggccag tcgccgtcgg gagtccactg cgtgcgcacg
     1981 ccctgaccgg cggcgacggc ggtcgccgcc agcccggcgc ggtcgaggtg ctcctggacg
     2041 gcgcgcacgc cctccggtcc gacgtcgtcg ggccgccggc tgacgctggt gaggacccgc
     2101 gggacgcggg cgacgcgctc gtcggacaga tcggcgcgca gcagcaggtc ccaggtcatc
     2161 gcgtcgccga agccgtcgcg cacgccgtgg acgaacagca ggcggctgcg gcggatcgcg
     2221 aagctcagcc cgacgtagtc ggcgccggtg agcaggtcgg gcgaccacga cggccggaac
     2281 cgcgggtcgg tgcgggcgcc gtcgacgagc cggtcgtcgt cccagacgac gaggtcgacg
     2341 agcgggtcgc ggtgcgaggt gagggccacc tggtacaggc agtcgggggc gagcaggctg
     2401 ccggcgtcgg cgagcacgac gaagtcggtc gcggcgttgc cgacggcggc gttgaccgcc
     2461 tcggacagct gaccggtgtg ccccgaccac accgagacgt cgtgcgactc gacgccgtcg
     2521 accaggacgg cggcggacgc cacggcgatc gaccagtgcg tccagctctg ctcgcggacg
     2581 ctcgcgatgg tgcggcggac ggcggcgac
//
LOCUS       DASWNJ010001992         1973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_458559, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001992 DASWNJ010000000
VERSION     DASWNJ010001992.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458559"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VGB85_33200"
     CDS             complement(<1..441)
                     /locus_tag="VGB85_33200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139007.1"
                     /translation="MPSPPRLFGALLTPLLLGCPGTGSEGSNSSSTTEHITSVTATDT
                     SSSDPTNTTPSTVTDTPTDTTSTSPCEDGAVQCTSVDERLVCKGGKWVDEPCAEGQGC
                     DGFGDCSPCTCEVSSCIDEDTINACSCFDVTPQACDAGTACDELN"
     gene            706..1407
                     /locus_tag="VGB85_33205"
     CDS             706..1407
                     /locus_tag="VGB85_33205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139008.1"
                     /translation="MVVLAALSVACEVASAPEIPAPIATAGPETCAPAEAVDFTWALG
                     PVAGDIARGVACVLGEVTADGGALVVPLECAEPEGARARTLAIQAAPPPPTAALRAGM
                     AVRLWAIAAVDEDGAVDRFVRLETASGGLLIAAAQAGALRPPDGSDPWLPFVITAAAS
                     ACMSEETACGSQERSAIDLRRAGGPPRVLHDQGFAAVGDRGEAQLWVTAAIVGDTACL
                     GASGASYGLGLIAAR"
     gene            1459..>1973
                     /locus_tag="VGB85_33210"
     CDS             1459..>1973
                     /locus_tag="VGB85_33210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0139009.1"
                     /translation="MIRARGVHKSFRTGKTELPVLRGVDLEVADGEFVAVVGRSGSGK
                     STLLGILGGLDTDYRGEVEVGGQDLRKLSDARLSAYRNASIGFVFQAFHLLDHLSCID
                     NVALPAMFVRGAAATSDTVARARAQELLERVGIPEKASAKPSTLSGGQKQRLAIARAL
                     FHRPALLIGDEP"
BASE COUNT          243 a          643 c          798 g          289 t
ORIGIN      
        1 gttgagctcg tcgcaggcgg tgccggcgtc gcaggcctgc ggcgtgacgt cgaagcagga
       61 gcacgcgttg atcgtgtcct cgtcgatgca gctcgagacc tcgcaggtgc acggcgaaca
      121 gtcgccgaag ccgtcgcagc cctggccctc ggcgcacggc tcatcgaccc acttgccgcc
      181 cttgcacacc agccgctcgt cgaccgaggt gcactgcacg gcgccgtctt cacacgggct
      241 cgtcgaggtg gtgtcggtcg gcgtatcggt gacggtcgac ggggtagtgt tggtcgggtc
      301 cgacgaggat gtgtcggtcg cggtgacgga ggtgatgtgc tcggtcgtgc tgctgctgtt
      361 ggagccctcg cttcctgtac ccgggcagcc gagcagcagt ggcgtcagca gcgcgccgaa
      421 gaggcgcggg ggcgagggca agcacgacat aggcgatcca tggtgccgcg ggccgctggg
      481 gcgcacaagt ccatgacaaa ttgatcaggc ctcgtcgggg tcgtcatgcc tccagcgcgg
      541 ggagtcatgg gtttttatgg ggggccacga gccgccaggc ggcgacgccg gtgagcgacc
      601 gcggttctgg acggatctcc gctgctcgcg cacactggct ccgtgaacct cggaccgcgc
      661 cgcgcggcgg accccgatcg gggtgtctgt gggcaggtca ggcgcgtggt cgtgctggcg
      721 gcgctgtcgg tcgcctgcga ggtcgcgtcg gctccggaga tcccggcacc gatcgcaacc
      781 gcggggccgg agacctgcgc gccggcagag gcggtcgatt tcacctgggc gctggggccc
      841 gtcgccgggg acatcgcgcg cggggtggcg tgtgtgctcg gcgaagtgac ggcggacggc
      901 ggggcgctgg tggtgccgct cgagtgcgcg gagcccgagg gcgcacgggc gcggacgctg
      961 gcgatccagg ccgctccgcc gccgccgacc gcggccctgc gcgccggcat ggcggtgcgg
     1021 ctgtgggcga tcgcggcggt cgacgaggac ggcgcggtcg accgcttcgt gcgcctcgag
     1081 acggcgagcg gcgggctgtt gatcgcggcg gctcaggcgg gggccctgcg cccgccggac
     1141 ggcagcgacc cgtggctgcc gttcgtgatc acggcggcgg ccagcgcgtg catgtccgaa
     1201 gagacagcct gcgggtcgca ggagcgctcg gcgatcgacc tgcgccgcgc gggcggtccg
     1261 ccgcgggtcc tgcacgacca gggcttcgcg gcggtcggcg accgcggcga ggcgcagctg
     1321 tgggtgacgg cggcgatcgt cggcgatacc gcctgcctcg gggccagcgg ggcgagctac
     1381 gggctggggc tgatcgcggc gcgctgaccc gctttcgcgg ttgacccccc gcgcggcccg
     1441 gcgttacgtt gcccgcgcat gatccgcgcg cggggcgtac acaagagctt tcgcaccggc
     1501 aagacggagc tgccggtgct gcggggcgtc gacctcgagg tcgcggacgg cgagttcgtg
     1561 gcggtggtcg ggcgctcggg ctcgggcaag agcacgctgc tcggcatcct cggcggcctc
     1621 gacaccgatt atcgcgggga ggtcgaggtc ggagggcagg acctgcgaaa gctgtcggac
     1681 gcgcgcctga gcgcctaccg caacgcgtcg atcggcttcg tgttccaggc gttccatttg
     1741 ctcgaccacc tgagctgcat cgacaacgtc gccttgccgg cgatgttcgt gcgcggcgcg
     1801 gcggcgacga gcgacacggt cgcgcgggcg cgggcgcagg agctgctcga gcgggtcggg
     1861 atcccggaga aggcgagcgc gaagccgtcg acgctgtcgg gcgggcaaaa gcagcgcctg
     1921 gcgatcgcgc gggcgctgtt tcaccggccg gcgctgctga tcggtgacga gcc
//
LOCUS       DASWNJ010001993         5587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_418675, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001993 DASWNJ010000000
VERSION     DASWNJ010001993.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5587
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418675"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VGB85_33215"
     CDS             complement(<1..544)
                     /locus_tag="VGB85_33215"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY0139010.1"
                     /translation="MTEASDSSQTPPLNTNSRLSAEVLWAMPVQERMVKLNWLGNMWR
                     SDSGTPYLAADFIGRLGRGVCLVDVREADELTGPTGHVPGTQWVPMTRIAELPELYSA
                     STPVVIVSRNGGPRAAEAARQLECAGMSFVAVMDGGIMAWKKLGFATVRDEAVLSRAP
                     QAPVVAPTTVGTPGPLSRAQI"
     gene            672..1670
                     /locus_tag="VGB85_33220"
     CDS             672..1670
                     /locus_tag="VGB85_33220"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY0139011.1"
                     /translation="MVRWVVVCAFASGLACTMSNPLFVVDEDPASGSTFDPSTTDPAT
                     GDPADPADPAQTTGTTGDGALPGTSTPDESSGDATSTSTEPATGDGTTATSSTDETTG
                     TGEPGPCQDVCGTPGCGDCPDAGMVAYPGFSIGAREVSNAEYQQFLAAGQDPAIQPEQ
                     CAWNDDYTPTIWPAIDPALPVVNIDWCDARAYCAWAGKRLCGAIEGGPASPFDIVNPA
                     KNQWYRACSNGDFRPYPYGKKYKKFACNGADALFGGLLGVGSLPLCEAPIAGVFDLSG
                     NVWEWVDSCIGDGPDAECMRRGGSFYSDPDALRCDVLSTRPRSTAVHYVGLRCCSL"
     gene            complement(1683..3080)
                     /locus_tag="VGB85_33225"
     CDS             complement(1683..3080)
                     /locus_tag="VGB85_33225"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY0139012.1"
                     /translation="MSVTDAGARAEETASASLVRGLGPIATTSLVVGNMIGSGIYVVP
                     AGLAEVAGPLGLVAWAINTAGYLCLTCVFADLGGAYPVSGGLQVFARRAFGDFAALEV
                     GYLYWMCAVIGNAAFLTAFITYLAVLWPGASEPMIAFAVSQALLWGLTLFNAAGVKVG
                     GVVQTVTVICKVVPLLLLSVALFAFASLDNLQPFAPHGAGSLLPAVSLVSWLFIGMES
                     VTVPAEEIRGAGKTIRSAAYTGFAVTCGVYLLVLLALNLAVPASELAGSGSPLAAVAR
                     AKFGEVGAVIVTIGALVSVAGILNGWLLVTSRMAYATARDGLAPRGLARINLHTGTPV
                     VALVTSSALTGGLVLMYFGGGLLEIYNFVALLSTATALVAIGVACLAEIVLVRREPER
                     FTPQQRRRGPITAGLGLGVVVLMIAGSGLEVIGLTIAAALLPLPYYFLVVRRSARAVL
                     ARAAGVSPWRARRKP"
     gene            complement(3077..4339)
                     /locus_tag="VGB85_33230"
     CDS             complement(3077..4339)
                     /locus_tag="VGB85_33230"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEY0139013.1"
                     /translation="MKQAQDELVWVDTNGVVHPLGESAGEQMRRRRDGAFRLMPTPDH
                     VIFMRYTGQDGRRDEEDGAVVRLAGEIVAPGAMCDVLALVAQAGWRGELTVRDGEHNR
                     SVFIENGNAISARSDVEEERLGRILYRYGAVDEVQHRAIYERLAQGSGERYGEAAVEL
                     GIVTHNQIFTYLAKQVEEVVLATMTVADGAYCFLDGFDESGLISRHSISLNGLLMDGV
                     TRMDEMRYFRQRIPTADYVPERIEGRPDPGKEFLTIWKAIDNQLSVHELGRVLGLGEF
                     ETTKLLFQLVQSKHVTMQRPRLRGGPLEVVETANAVLREIHQEADTAGKGTILRQQLA
                     AFADGAYEMVFANAGPYEKGTFLAQRVVDNSRAMAAGGSVEKLLKELLYDYVSFALFT
                     AGSLVRRPKDQGIGKQVDPLMSRLRPLG"
     gene            4418..>5587
                     /locus_tag="VGB85_33235"
     CDS             4418..>5587
                     /locus_tag="VGB85_33235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139014.1"
                     /translation="MPRLAANSHVRSLAALVSLTCVALTCVADLACATAAPAAVPSPR
                     GRDCRNPAQTLQDGPNPVWNAARRRAVTRAFDNLPGPPSAGPRLAGRLDAQATAWIKA
                     RTETCRAARHRRGASVEYTAAAACQHGVLLQHAALVRRITQAPADSLVRADAGIDELE
                     DALRRCGRPAIRDLHRGDAELLTARAELSVADLAIAMQDGSWAQAVSHTASEPAVSAA
                     VAAALRPQVQLSLAWGSWLRGQDQKASSRLGELVGGDPLTRANLAELRLFTRGIDDPE
                     ALRDGAAALAAYHALLGPGDRRVVRVHRELARRHRLAGRIDAAVAELEAALKDPGPGA
                     DDPLHAALTQELGDLEHLRGDYRTAALRHQAALDARTRSFGSEQLPTAESLFALGN"
BASE COUNT          829 a         2225 c         1810 g          723 t
ORIGIN      
        1 cgatctgcgc gcgcgacagc gggcccgggg tcccgaccgt cgtcggcgcc acgaccggag
       61 cctgcggcgc gcgcgacagc accgcctcgt cgcgcacggt cgcaaagccg agctttttcc
      121 aggccatgat cccgccgtcc atcacggcga cgaagctcat gccggcgcac tcgagctggc
      181 gcgccgcctc ggctgcccgc gggccgccgt tgcgcgacac gatgaccacc ggcgtgctcg
      241 ccgaatacag ctcgggcagc tcggcgatgc gcgtcatcgg cacccattgc gtgccgggca
      301 cgtggcccgt cgggcccgtc agctcgtcgg cctcgcgcac gtcgaccaga cacacgccgc
      361 gcccgagccg cccgatgaag tccgcagcga ggtagggcgt gccgctgtcg gaccgccaca
      421 tgttgccgag ccagttgagc ttgaccatgc gctcctgcac tggcatcgcc cacagcacct
      481 ccgcggacag gcgcgagttg gtgttgagcg ggggggtctg ggacgagtcg ctggcttcag
      541 tcatcggcgc gcgaaccata gcgcgtctgg gtcctgcggt ggatggcccc ccacggcgac
      601 acgcgcgccg agcgaaaaga cccgcgagat tgatcgtgcc agcgccccgg ccccggtgat
      661 agcgtccggt catggttcgc tgggtggtgg tgtgcgcgtt cgcctcggga ctcgcctgca
      721 cgatgtccaa tccgctgttt gtcgtcgacg aggatccggc cagcggatcc accttcgacc
      781 cgtccacgac cgatcctgcc accggcgatc ccgcggaccc cgcagacccc gcgcagacca
      841 ccgggaccac cggcgacggc gcgctgcccg ggacctccac ccccgacgag agcagcggcg
      901 acgccacgag cacgagcacg gagcccgcca ccggcgacgg caccactgcc accagctcca
      961 ccgacgaaac caccggcact ggcgagccgg gcccgtgcca ggacgtgtgc ggcacgccgg
     1021 gctgcggcga ctgccccgac gccggcatgg tcgcctaccc cggcttttcg atcggcgcgc
     1081 gcgaggtcag caacgccgaa tatcagcagt tcctcgccgc cgggcaggac ccggcgatcc
     1141 agcccgagca gtgcgcatgg aacgacgact acacgccgac gatctggccg gcgatcgacc
     1201 ccgcgctgcc ggtcgtcaac atcgactggt gcgacgcgcg ggcctactgc gcctgggccg
     1261 gcaagcgcct gtgcggtgcg atcgagggcg gcccggcttc ccccttcgac atcgtcaatc
     1321 ccgcgaaaaa tcagtggtac cgcgcctgct ccaacggcga ctttcggccc tacccctacg
     1381 gtaaaaaata caaaaagttc gcctgcaacg gcgccgacgc cctgttcggc ggcctgctcg
     1441 gcgtcggctc gctacctctc tgcgaggctc cgatcgcggg cgtgttcgac ctcagcggca
     1501 acgtgtggga gtgggtcgac agctgcatcg gcgacggccc cgacgccgag tgcatgcgcc
     1561 gcggcggctc gttctacagc gaccctgacg ccctgcgctg cgacgtgctc agcacccgcc
     1621 cgcgcagcac cgccgtgcat tacgtcggcc tgcgctgttg cagcctgtga tcctgtgacc
     1681 gctcagggct tgcgtcgggc tcgccacggc gagaccccgg ccgcgcgcgc gagcacggcg
     1741 cgggcgctgc ggcgcacgac caggaagtag tacggcagcg gcagcagggc cgccgcgatc
     1801 gtcaaaccga tgacctcgag ccccgagccc gcgatcatca gcacgaccac gccgaggccg
     1861 aggcccgcgg tgatcggccc gcgccggcgc tgctgcggcg tgaagcgctc gggctcgcgg
     1921 cgcaccagca cgatctccgc caggcacgcg acgccgatcg ccaccagcgc ggtcgccgtc
     1981 gagagcagcg cgacgaagtt gtagatctcg agcaggccgc cgccgaagta catcaatacc
     2041 aggccgccgg tcagcgccga cgacgtcacc agcgcgacca ccggcgtgcc cgtgtgcagg
     2101 ttgatccgcg ccagcccgcg cggcgccagc ccgtcgcgcg ccgtcgcgta ggccatgcgc
     2161 gaggtgacca gcagccaccc gttcaaaatt cccgcgaccg acaccagcgc gccgatcgtc
     2221 acgatcaccg cgccgacctc cccgaacttg gcgcgggcga ccgccgccag cgggctgccc
     2281 gagccggcca gctcgctcgc cggcaccgcg aggttgagcg ccagcagcac cagcaggtac
     2341 acgccgcagg tcaccgcgaa gccggtgtag gccgcgctgc ggatcgtctt gcccgcgccg
     2401 cggatctcct ctgctggcac cgtcaccgac tccatgccga tgaacagcca cgataccagc
     2461 gacaccgccg gcagcagcga gcccgcgccg tgcggcgcga acggctggag gttgtcgagc
     2521 gacgcgaacg cgaacaacgc gacgctgagc agcagcagcg gcaccacctt gcagatcacc
     2581 gtcaccgtct gcaccacgcc gccgaccttg acccccgccg cgttgaacag cgtgagcccc
     2641 cacagcagcg cctggctgac cgcgaacgcg atcatcggct cggacgcgcc gggccacagc
     2701 accgcgaggt aggtgatgaa cgcggtcagg aacgccgcgt tcccgatcac cgcgcacatc
     2761 cagtacaggt agccgacctc gagcgccgcg aagtcgccga aggcccggcg cgcgaacacc
     2821 tgcaggccgc ccgacaccgg gtacgcgccg ccgaggtccg cgaacacgca ggtcaggcac
     2881 agatacccgg ccgtgttgat cgcccacgcg accagcccga gcggccccgc gacctcggcg
     2941 agcccggccg gcaccacgta gatccccgag ccgatcatgt tgccgacgac cagcgaggtc
     3001 gtggcgatcg gcccgagccc gcgcaccagc gaggcgctcg ccgtctcctc ggccctcgcg
     3061 cccgcgtccg tcacgctcat cccagcggcc gcagccggct catcaagggg tcgacctgct
     3121 tgccgatccc ctggtccttc gggcgccgca ccagactgcc cgcggtgaac agcgcgaagc
     3181 tgacgtagtc gtacagcagc tccttgagca gcttctcgac gctgccgccg gccgccatcg
     3241 cgcgcgagtt gtcgaccacg cgctgggcca ggaacgtgcc cttttcgtac ggcccggcgt
     3301 tggcgaacac catctcgtag gcgccgtcgg cgaacgcggc cagctgctgg cgcaggatcg
     3361 tgcccttgcc ggccgtgtcg gcctcctggt ggatctcccg cagcaccgcg ttggcggtct
     3421 cgacgacctc gagcgggccg ccgcgcaggc gcgggcgctg catcgtcacg tgcttcgact
     3481 gcaccagctg gaacagcagc ttggtcgtct cgaactcgcc gagcccgagc acccgaccga
     3541 gctcgtgcac gctcagctgg ttgtcgatcg ccttccagat cgtgaggaac tctttgcctg
     3601 gatccgggcg gccttcgatc cgctccggca cgtagtcggc cgtcgggatg cgctggcgga
     3661 agtatcgcat ctcgtccatg cgcgtcacgc cgtccatcag cagcccgttg aggctgatgc
     3721 tgtggcgcga gatcaggccg ctctcgtcga agccgtcgag gaagcagtac gcgccgtcgg
     3781 cgacggtcat cgtcgccagc acgacctcct cgacctgctt cgccaggtac gtgaagatct
     3841 ggttgtgcgt cacgatgccg agctcgaccg ccgcctcgcc gtagcgctcg ccgctgccct
     3901 gcgcgagccg ctcgtagatc gcccggtgct gaacctcgtc gaccgcgccg tagcggtaca
     3961 agatccgtcc gagccgctcc tcctcgacgt cgctgcgcgc cgagatcgcg ttgccgttct
     4021 cgatgaagac gctgcggttg tgctcgccgt cgcggaccgt cagctcgccg cgccagccgg
     4081 cctgggccac cagcgccagc acgtcacaca tcgcacccgg cgcgactatt tcgcccgcga
     4141 ggcggaccac cgcgccgtcc tcctcgtcgc gccgaccgtc ctggccggtg tagcgcatga
     4201 agatcacgtg gtcgggcgtc ggcatcaggc gaaacgcacc atcccggcgc cgacgcatct
     4261 gctcgccggc cgactccccg agggggtgca cgaccccgtt cgtgtcgacc cagaccagct
     4321 cgtcttgcgc ttgtttcatg gccgtccagc ctccgacacc gcttggtaac accggatcgc
     4381 ccagcgccgc aagttgcgcc ggagtatacc tcgccccttg ccgcgactcg ccgccaactc
     4441 ccacgtccgc tcgctcgccg cgctcgtgag cctcacctgc gtggccctca catgcgtcgc
     4501 cgacctcgcc tgcgccaccg cagcgcccgc cgccgtcccc agccctcgcg gacgcgactg
     4561 ccgaaacccc gcgcagaccc tgcaagacgg cccgaacccg gtctggaacg ccgcgcgacg
     4621 cagggccgtc acgcgggcct tcgacaacct ccccggcccg cccagcgccg gcccccgcct
     4681 cgccgggcgg ctcgacgcgc aggcgaccgc ctggataaag gcgcgcaccg agacgtgccg
     4741 cgccgcacgt caccgccgcg gcgcctcggt cgagtacacc gccgcggccg cgtgccagca
     4801 cggcgtgctc ctgcaacacg ccgcgctcgt ccggaggatc acgcaggccc cggccgacag
     4861 cctcgtccgc gccgacgccg gcatcgacga gctggaggac gcgctgcggc gctgcgggcg
     4921 gccagcgatc cgcgacctcc accgcggcga cgccgagctg ctgaccgccc gcgccgagct
     4981 ctccgtcgcc gacctggcga tcgccatgca ggacgggtcg tgggcccagg ccgtctccca
     5041 caccgccagc gaacccgccg tgtccgccgc cgtcgccgcc gcgctgcgac cccaggtcca
     5101 gctgtccctg gcctgggggt catggctccg aggacaggac caaaaggcca gctcgcgcct
     5161 cggcgagctc gtcggcggcg acccgctcac ccgggccaac ctcgccgagc tgcgcctgtt
     5221 cacccgcggc atcgacgacc ccgaagccct ccgcgacggc gccgccgcgc tcgccgccta
     5281 tcacgccctg ctcggccccg gcgaccgccg cgtcgtccgc gtccaccgcg agctcgcccg
     5341 ccgccaccgc ctcgccggcc gcatcgacgc cgccgtcgcc gagctcgagg ccgccttgaa
     5401 ggacccgggc cccggcgccg acgacccgct gcacgccgcg ctgacgcagg agctcggcga
     5461 cctcgagcac ctgcgcggcg actaccgcac cgccgcgctg cgccaccagg ccgcgctcga
     5521 cgcccgcacg cgcagcttcg gcagcgagca gctgccgacc gccgagagcc tgttcgcgct
     5581 cggcaac
//
LOCUS       DASWNJ010001994         2717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_347145, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001994 DASWNJ010000000
VERSION     DASWNJ010001994.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347145"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..667)
                     /locus_tag="VGB85_33240"
     CDS             complement(173..667)
                     /locus_tag="VGB85_33240"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEY0139015.1"
                     /translation="MTDSERRNPPASSPGPSRDESPARPWTRREALGAGLGVVSLAVV
                     GCARLPPVRDVPSSGGEVRMALVDYPDLQKAHGVVPVRPDGRGKPIMVVRGEGEQFTA
                     LSLRCTHMGCTVGWDDEARRFQCPCHGSRFAADGGVLKGPAKRPLTGYPVSFDGQTLR
                     FMVG"
     gene            683..1687
                     /locus_tag="VGB85_33245"
     CDS             683..1687
                     /locus_tag="VGB85_33245"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEY0139016.1"
                     /translation="MRQPARGRKPVSLAAVMASTIMALGSAPACERPPAAVPGPADAT
                     TGEQVPGAAFSGERAHERVAALLALPRSLGHPQRASSIDALDGLLRAAGASSVGRLEH
                     TGADPWTRTQFAMTTLIAHVRPQAPRQFVLATHFDVRPWAESDPDPTRREQAIPGAND
                     GTSGVAVLLELAPLLLDQLPQDVGFSLILFDGEELGRPGDGPYCAGSRALAQAIARGE
                     FPEVRAARFGVVLDMVGDADLQLLQDPRSRRAAAAIVDEIWSTGQAAGFQQFVAGPGP
                     ELLDDHVALTEAGVPSLLLIDYSYPAWHTHADDLSQISAASLGAVGETLRRALRAHRW
                     "
     gene            complement(1692..>2717)
                     /locus_tag="VGB85_33250"
     CDS             complement(1692..>2717)
                     /locus_tag="VGB85_33250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139017.1"
                     /translation="FGRPGEIGDPPAWRSTMSSPRTQLLAPLAALTVLVGIGVGWAHA
                     GSTLSSCLAECKRSHLSVTNRATCRLDCEVDAASDPEVIQAQTASLRPPVPRPASRPV
                     KPPAPAPVASSARPGDPGCKAACDADAALSADDRATCKLECDLVPDPMPTGTPPPMRP
                     PSAAALASARTGTAPTTTTPGAAVARTAADRPGFLARCHATCKPGRGEREATDYESCK
                     LDCNNMASVLDVASVWVPEAWDGRPAVAAPPVVPVEPGPVVTRPAPPRRAAPAPARAT
                     CGPTLEKCRASCVTSETKCAQKCSRKHIQETDRETCKLGCGTDQEVCQGDCLSATATC
                     VNDSNPR"
BASE COUNT          335 a          992 c         1042 g          348 t
ORIGIN      
        1 agattgccga ggcggtcgac cgcgtaggcg gtgagcccga ccagcagacc atcctcgcgg
       61 cgctcgaccc aggtgtgatc gcggctgtaa cggcgctcgg gagccatgcg cgggcagcat
      121 gacccagggg gcctcggacc acaagcgtcg ccgcgggtcc cgtcccctgc cctcagccga
      181 ccatgaagcg cagcgtctgc ccgtcgaagc tgaccggata accggtcagc gggcgcttcg
      241 cgggcccctt gagcacgccg ccgtcggccg cgaagcgcga gccgtggcac gggcactgga
      301 accgccgcgc ctcgtcgtcc cagccgaccg tgcagcccat atgcgtacag cgcagcgaca
      361 gcgcggtgaa ctgctcaccc tcgccgcgca cgaccatgat cggcttgccg cggccgtccg
      421 ggcgcaccgg gaccacgccg tgggcctttt gcagatcggg gtagtcgacg agcgccatcc
      481 ggacctcgcc gccgctgctc ggcacgtcgc gcacgggcgg gagcctggcg cagccgacca
      541 ccgcgaggct gacgacgccg aggccggcgc cgagcgcctc gcgccgggtc cacgggcggg
      601 ccggagattc gtcacgcgac gggcccggag aggacgcggg ggggtttcgc cgttcggagt
      661 ccgtcatggt agccccccca ctatgagaca gcccgcgcgc ggacggaagc cggtcagcct
      721 cgccgcggtc atggcgagca cgatcatggc gctcgggtcg gcccccgcgt gcgagcgtcc
      781 gcccgcggcc gtgcccggtc cggccgacgc gacgaccggc gaacaggtcc ccggcgcggc
      841 gttctcgggc gagcgagccc acgagcgcgt cgcggccctg ctcgcgctgc cgcggtcgct
      901 cgggcacccg cagcgcgcct cctcgatcga cgcgctcgac gggctgctgc gggccgcggg
      961 cgcgagctcc gtgggccgcc tcgagcacac cggcgcggac ccgtggacgc gcacgcagtt
     1021 cgcgatgacg acgctgatcg cacatgtgcg cccgcaggcg ccgcggcagt tcgtgctcgc
     1081 gacccacttc gacgtccgcc cctgggccga atccgacccc gacccgacgc gccgcgaaca
     1141 ggcgatcccc ggggccaacg acggcaccag cggggtggcg gtgctgctcg agctggcgcc
     1201 gctgttgctc gatcagctcc cgcaggacgt cggcttctcg ctgatcctgt tcgacggcga
     1261 ggagctcggg cgccccggcg acggccccta ctgcgccggc tcgcgcgcgc tcgcgcaggc
     1321 gatcgcccgc ggcgagttcc ccgaggtccg cgcggcccgc ttcggcgtcg tgctcgacat
     1381 ggtcggcgac gcggacctgc agctgctgca ggacccgcga tcgcggcgcg cggccgcggc
     1441 gatcgtcgac gagatctggt cgaccggaca ggccgccggc ttccagcagt tcgtcgccgg
     1501 gccagggccc gagctgctcg acgatcacgt cgcgttgacc gaggcggggg tgccctcgct
     1561 gctgctgatc gattacagct acccggcctg gcacacccac gcggacgacc tctcgcagat
     1621 ctcggcggcg agcctcggcg ccgtcggcga gaccctgcgc cgcgcgctgc gagcccaccg
     1681 ctggtgacgc gtcagcgcgg gttgctgtcg ttgacgcagg tggcggtcgc gctcaggcag
     1741 tcgccctgac acacctcctg gtcggtgccg cagccgagct tgcaggtctc gcggtcggtc
     1801 tcctggatgt gcttgcggct gcacttctgc gcgcacttgg tctcgctcgt cacgcagctg
     1861 gcgcggcact tttcgagggt cgggccgcag gtcgcacgcg ctggcgctgg cgcggcgcgg
     1921 cgcggcggcg cgggccgggt cacgaccggc cctggctcga cagggaccac cggcggggcc
     1981 gcgaccgccg ggcggccgtc ccacgcctcg gggacccaca ccgaggcgac gtcgagcacg
     2041 ctcgccatgt tgttgcagtc gagcttgcag gactcgtaat cggtcgcctc gcgctcgccg
     2101 cggccgggct tgcaggtcgc gtggcacctg gccaaaaagc caggtcggtc ggcggccgtc
     2161 cgcgcgaccg cggcaccggg tgtggtggtc gtcggcgcgg tgccggtgcg ggccgacgcg
     2221 agggccgcgg cggacggcgg gcgcatcggc ggcggcgtgc cggtcggcat cgggtcgggc
     2281 accaggtcgc actcgagctt gcaggtcgcg cggtcgtcgg cagacagcgc cgcgtcggcg
     2341 tcgcaggccg ccttgcagcc cgggtcaccg gggcgggcag aggatgccac gggcgcgggc
     2401 gcgggtggct tgacgggccg ggacgcgggg cgcggcacgg gcgggcgcag cgaggcggtc
     2461 tgggcctgga tcacctcggg gtcgctggcg gcgtcgactt cacagtcgag gcggcaggtc
     2521 gcgcggttgg tgaccgacag gtgcgatcgc ttgcactccg cgaggcagct ggagagggtc
     2581 gagccggcgt gggcccagcc gacgccgatc ccgacgagga ccgtgagggc ggcgagcggc
     2641 gcgaggagct gcgtgcgagg cgaggacatg gttgatctcc aggccggagg atctccgatc
     2701 tcaccggggc gaccaaa
//
LOCUS       DASWNJ010001995         1835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_347186, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001995 DASWNJ010000000
VERSION     DASWNJ010001995.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347186"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VGB85_33255"
     CDS             complement(<1..402)
                     /locus_tag="VGB85_33255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY0139018.1"
                     /translation="MDPACFKDPAAGLRPHFEGPSQPPRILLLHGSARDRSYSRLASE
                     EAARILRSMGAETRAFDPRGMPLPDDAPETHPKVQELRELAAWSEGMVWSSPERHGAM
                     TGIMKAQIDWIPLTVGAVRPTQGKTLALMQVS"
     gene            489..851
                     /locus_tag="VGB85_33260"
     CDS             489..851
                     /locus_tag="VGB85_33260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY0139019.1"
                     /translation="MKAMQTLEVKDALDEADAVKALGALAQAHRLRVFRALVVAGPEG
                     MTPGALGTALDVAPSALSFHLKELSIAGLLDAEQRGRNIVYRARFERMNALLAYLTEH
                     CCQGQECAASAAPCCDTD"
     gene            872..1345
                     /locus_tag="VGB85_33265"
     CDS             872..1345
                     /locus_tag="VGB85_33265"
                     /inference="COORDINATES: protein motif:HMM:NF041414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HEY0139020.1"
                     /translation="MKRFHVHMHVDDLAKNVAFYSAMFGQEPARTEGDYAKWMLQDPP
                     VNFAISTRGDKPGVDHLGIQVESAEELTAMKANAQKADMALMDEGETTCCYARSDKYW
                     VTDPQGVAWEQFQTLDSIPVFSQKAEPKAGAEAAACCAPKGKPLGIPVKAAGSCC"
     gene            1350..1562
                     /locus_tag="VGB85_33270"
     CDS             1350..1562
                     /locus_tag="VGB85_33270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139021.1"
                     /translation="MSAARNQAGCCRQQTGNRASRGIGATLAGVAVATLAVVALLAAR
                     LAPFLPLGSETDARASGSGAATQPCC"
     gene            1571..>1835
                     /locus_tag="VGB85_33275"
     CDS             1571..>1835
                     /locus_tag="VGB85_33275"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEY0139022.1"
                     /translation="MTDRTYNALFICTGNSARSIIAEAILNDASRGRFKAFSAGSHPK
                     GEVHPLAIEELTRLRIPTDGLRSKGWEEFAQPGAPSMDFVFTVC"
BASE COUNT          304 a          629 c          624 g          278 t
ORIGIN      
        1 cgagacctgc atcaaagcca gcgtcttgcc ctgggtgggg cgcacggctc caacggtgag
       61 cggtatccag tcgatctgag ctttcatgat ccccgtcatg gccccgtggc gctcgggcga
      121 gctccagacc atgccttcgg accaggcggc gagctcgcgc agctcctgga ccttggggtg
      181 cgtctccggc gcgtcgtcgg gcaacggcat gccgcgggga tcgaacgcgc gggtctcggc
      241 gcccatggag cgcaggatcc tggcggcctc ctcgcttgct agccggctgt aggagcggtc
      301 ccgggcggag ccgtgcagca gcaggatcct gggcggttgg gaaggccctt cgaaatgggg
      361 ccgcaggccc gcggccgggt ctttgaagca ggcgggatcc atgttcggga ggtcggccgc
      421 ggcgaagggt tcgggttgtc gttgggtcat cgcttgcgtt ctttcaatgt ttcaattatc
      481 gtcggattat gaaagcaatg cagacgctag aagtcaagga cgccttggat gaggccgacg
      541 ctgtcaaggc gctcggcgcg ctcgcccagg cccaccgctt gcgggtgttc cgcgccttgg
      601 tggtcgcggg ccccgagggc atgactccgg gcgcccttgg aacggcgttg gatgtggccc
      661 ccagcgcgct ctccttccac ttgaaagagc tctcgatcgc cggcttgctg gacgccgagc
      721 aacggggccg aaacatcgtc taccgggcgc gcttcgagcg gatgaacgcc ctgctcgcct
      781 acctcaccga gcactgctgc caagggcagg agtgcgcagc ctccgcggcg ccctgctgcg
      841 acaccgactg acccacccca aggagagagc catgaagaga ttccacgtcc acatgcacgt
      901 cgacgacctg gcgaagaacg tcgccttcta ctccgccatg ttcgggcagg agcccgcgcg
      961 caccgagggc gactacgcca aatggatgct gcaggacccg ccggtgaact tcgcgatctc
     1021 cacgcgcggc gacaagcccg gcgtcgacca cctgggcatc caggtcgagt cggccgagga
     1081 gttgaccgcg atgaaggcca acgcccaaaa ggcggacatg gcgctgatgg acgagggcga
     1141 gacgacctgc tgctacgcgc gcagcgacaa atactgggtg accgacccgc agggcgtcgc
     1201 ctgggagcag ttccagacgc tggactcgat cccggtgttc agccagaaag cggagcccaa
     1261 ggcgggagcg gaagccgccg cctgctgcgc gcccaagggc aagccgctgg gcatcccggt
     1321 caaagccgcc ggctcctgct gctgacgcca tgagcgccgc tcgcaaccag gccggctgct
     1381 gccgccaaca aacaggcaac cgcgcctcac gcgggatcgg cgccactctc gccggagtcg
     1441 cggtggccac gctcgccgtc gtggcgctgc tcgccgcgcg cctcgctccg ttcctgcccc
     1501 ttggatccga gaccgacgcg agggccagcg gctccggcgc cgcgacgcag ccgtgctgct
     1561 gaaagaccgc atgaccgacc gaacctacaa cgcgctgttc atctgcaccg gcaactcggc
     1621 gcgatccatc atcgcggagg cgatcctgaa cgacgcgagc cgcggccgct tcaaggcgtt
     1681 ttccgccggc agccacccga agggcgaggt gcacccgctc gcgatcgagg agctgacgcg
     1741 cctccgcatc cccaccgacg gcctgcgcag caagggctgg gaggagttcg cgcagccggg
     1801 cgcgccttcg atggacttcg tcttcacggt gtgcg
//
LOCUS       DASWNJ010001996         1591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_387743, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001996 DASWNJ010000000
VERSION     DASWNJ010001996.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387743"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /gene="atpD"
                     /locus_tag="VGB85_33280"
     CDS             <1..1009
                     /gene="atpD"
                     /locus_tag="VGB85_33280"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY0139023.1"
                     /translation="LFGGAGVGKTVLLMELINNVGKKFGGYSVFAGVGERTREGNDLY
                     FEMIESGVIKADWEYETRLVRSVDTDKSKVALVYGQMNEPPGARARVALSGLTQAEYF
                     RDEMGQDVMLFVDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATEMGALQERITTTTK
                     GSITSVQAIYVPADDLTDPAPATAFAHLDATTVLSRALTEIGIYPAVDPLDSTSTILS
                     AEVVGQDHYDTARKVQQTLQKYKELQDIIKILGMDELSEEDKLIVGRARRVQRFLSQP
                     FDVAKVFTGNEGKFVELADTIQSFREIVEGKHDHLPEEAFYMVGGISEAIEKAKRLAG
                     G"
     gene            1040..1456
                     /gene="atpC"
                     /locus_tag="VGB85_33285"
     CDS             1040..1456
                     /gene="atpC"
                     /locus_tag="VGB85_33285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEY0139024.1"
                     /translation="MAATTIKLDILTPMGPRRRGLEVTGVEIPGVAGELGVLPDHVPF
                     VTPVVPGVVRFREGDTSVRIAVGAGFLEIGGDGRVAILVERALESGEIDVTSARNELS
                     RVNAELATQQDSIDAAQHRKLSTEQGWLEAQIRAAT"
BASE COUNT          269 a          567 c          529 g          226 t
ORIGIN      
        1 cctgttcggc ggcgccggcg tcggcaagac cgtgctcctg atggagctga tcaacaacgt
       61 cggcaagaag ttcggcggct actccgtgtt cgccggcgtc ggcgagcgca cccgcgaggg
      121 caacgacctg tacttcgaga tgatcgagtc cggcgtcatc aaggccgact gggagtacga
      181 gacgcgcctg gtccgctcgg tcgacaccga caagtccaag gtcgcgctcg tctacgggca
      241 gatgaacgag ccccccggcg cccgcgcccg cgtcgcgctg tccggcctga cccaggccga
      301 gtacttccgc gacgagatgg gccaggacgt catgctcttc gtcgacaaca tcttccgctt
      361 cacgcaggcg ggctccgagg tgtcggcgct gctcggtcgc atcccgagcg ccgtcggtta
      421 ccagccgacc ctggcgacgg agatgggcgc gctgcaggag cgcatcacga cgacgaccaa
      481 ggggtcgatc acctcggtgc aggcgatcta cgtgcccgcg gacgacctca ccgacccggc
      541 gcccgcgacg gccttcgccc acctcgacgc caccacggtg ctcagccgcg cgctgaccga
      601 gatcgggatc tacccggccg tcgatccgct cgactcgacc tcgacgatcc tcagcgccga
      661 ggtcgtcggc caggaccact acgacaccgc gcgcaaggtc cagcagaccc tccagaagta
      721 caaggagctg caggacatca tcaagatcct cggcatggac gagctcagcg aggaggacaa
      781 gctgatcgtc ggccgcgccc gtcgtgtgca gcgcttcctc agccagccct tcgacgtcgc
      841 caaggtgttc accggcaacg agggcaagtt cgtcgagctc gcggacacca tccagagctt
      901 ccgcgagatc gtcgagggca agcacgacca cctcccggag gaggcgttct acatggtcgg
      961 cggcatcagc gaggccatcg agaaggccaa gcgcctcgcc ggcggctgat cgcaatcgca
     1021 atagacaggg agcacgacca tggcggccac gacgatcaaa ctcgacatct tgaccccgat
     1081 gggtccgcgc cgccgcggcc tcgaggtgac gggcgtggag atccccggcg tcgccggcga
     1141 gctcggcgtc ctgcctgacc acgtgccgtt cgtgacgccg gtcgtgccgg gcgtggtccg
     1201 ctttcgtgag ggtgacacct ccgtgcggat cgcggtcggc gccggcttcc tcgagatcgg
     1261 cggcgacggc cgcgttgcga tcctggtcga gcgcgcgctc gagtccggcg agatcgacgt
     1321 caccagcgcc cgcaacgagc tgagccgcgt caacgccgag ctggccaccc agcaggactc
     1381 gatcgacgcg gcccagcacc gcaagctcag cacggagcag ggctggctcg aggcgcagat
     1441 ccgcgccgcg acctgagtcg ccactccacc ttcgagggcg cgtggcatcc gccgcgcgcc
     1501 ctcgtctcgt tcggtgcgcc gcgcgtatgt cccgagagac aggcgggagg ttcgccgcgc
     1561 gcgtatctgc cagacaatgc gccagtggcg g
//
LOCUS       DASWNJ010001997         4496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_140927, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001997 DASWNJ010000000
VERSION     DASWNJ010001997.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140927"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VGB85_33290"
     CDS             <1..627
                     /locus_tag="VGB85_33290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139025.1"
                     /translation="GGASSEGPRRPTPAAPAPSKPPTPPWQPAPAAPAPQPSPAVTPA
                     ADCGSGCPGSPQPERAGTIPWADMPPMAAWEAFLDRLRGNEGPMFGILAELGLAKLGD
                     GVLRLAGTATDFARNHLREQAPLRSHLESLLQQHLGLPFKLELVEGEPSLPDLPSLGL
                     VEQRRAEELQRKVVLEVENSPQIQALKAQFNAQVRSVKPRAAPQSRGF"
     gene            713..1039
                     /locus_tag="VGB85_33295"
     CDS             713..1039
                     /locus_tag="VGB85_33295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEY0139026.1"
                     /translation="MSEFDLGAMMRQAQQLREQMETMQKSLDKEIVEGTAGGGMVKAK
                     ATGTQQILSIELAPAALQEEREMLQDLIVAAVNNALERSKALTQQKVGSLLPPGLMGP
                     GGIPGL"
     gene            1036..1644
                     /gene="recR"
                     /locus_tag="VGB85_33300"
     CDS             1036..1644
                     /gene="recR"
                     /locus_tag="VGB85_33300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEY0139027.1"
                     /translation="MSGGGDTQNPLQRLAALLTRLPGVGEKSALRLALALVRSDSEYV
                     RALSEAIGAIHAELRLCATCCDLCTAEICERCRDPRRDPSLVCVVAQPQDRMAIERTG
                     AYSGLYHVLHGVLDPLAGIGPAELKIDALLRRLRGDAVREVIVATSPNVEGDATALYL
                     SKLLQPLGVRVTRIASGVAVGGELEYADLNTLHRALAERRSL"
     gene            1952..2434
                     /locus_tag="VGB85_33305"
     CDS             1952..2434
                     /locus_tag="VGB85_33305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEY0139028.1"
                     /translation="MNPQLVMFEEELKQIQKIVDRLLSDSRSRSILLIDKNGQLVAAS
                     GSAHELDTTSLSSLVAGNVAATGGIAKLLQEDEFTGQFHEGKGMSINLSLVGRRIILV
                     VLFDKNTTPGLVRLRVKRAQDELVPIFEALATKAAQPNGGSPALQEITDQDIDDLFND
                     "
     gene            2455..3042
                     /locus_tag="VGB85_33310"
     CDS             2455..3042
                     /locus_tag="VGB85_33310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HEY0139029.1"
                     /translation="MSFINYSSREINCKLVYYGPGLCGKTTNLQYIYNKTRPEAKGKM
                     ISLATETERTLFFDFLPLSLGEIRGFKTRFHLYTVPGQVFYDASRKLILKGVDGVCFV
                     ADSQEERYDANIESLENLRLNLQEQGYDLDKLPYVVQYNKRDLPNAMSVAELSKMLNP
                     TQVPEFEGVATTGIGVFDTLKMLAKLVLAELRKGT"
     gene            3409..4482
                     /locus_tag="VGB85_33315"
     CDS             3409..4482
                     /locus_tag="VGB85_33315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139030.1"
                     /translation="MPTEDWIQESLARLESLEEERNRHEAALETSTDPAALRMNAQAI
                     ERLDAKIRSLYAELEAAAEQGDAEQGDAVDTDLGDDPDLGDEAEGEDDVAGGTTQMFR
                     RDDLAAQAAAMRTSPETVRSVPASPKIGPAAAAAPAAAARVTPVAAAAVAAATAPTAS
                     PFSAPVSTPAPVSSAPAASPFGGAAAASAPTASPFGNSASPFGEVPQAAPFGNSGDSA
                     GFSAPATSNSSFDAGNSFDDESSSGSGSKMGIIIGVVVVVLGLGAYLAFGGKGDEPPP
                     AAPAGPVKVIGGLEVPPDTQGPKTVQGSSDAIKGTQYKEGRPQSGGGGGGSNAPRPSD
                     KPVKKDPRAKIQNADDPLAGIDK"
BASE COUNT          776 a         1563 c         1490 g          667 t
ORIGIN      
        1 ggcggcgcga gctcggaggg tccgcgccgg ccgaccccgg ccgcgcccgc gccaagcaag
       61 ccgcccacgc ccccctggca gcccgcaccg gccgcgcccg cgccccagcc ctcgcctgcc
      121 gtcacgcccg cggccgactg cggcagcggt tgtccgggat cgccgcagcc tgagcgcgcc
      181 ggcacgatcc cctgggccga catgccgccg atggccgcct gggaggcttt tttggatcgc
      241 ctgcgtggca acgaggggcc gatgttcggc attttggccg agctcggcct ggccaagctc
      301 ggcgacggcg tcctgcgtct ggccggcacg gccacggact tcgcccgcaa tcacctgcgc
      361 gagcaagccc cgctgcgctc gcacctcgag tcgttgctgc agcaacacct cgggttgccg
      421 ttcaagctcg agctcgtcga gggtgagccg agcttgccgg atctgccgag cctggggctg
      481 gtcgagcagc ggcgggcgga ggagctgcag cgaaaagtcg tcctcgaggt cgagaacagc
      541 ccgcagatcc aggcgctcaa ggcccagttc aacgcccagg tccgcagcgt caagccgcgt
      601 gcagcgccgc aatcccgggg tttttaggcc cggagctttc aggcccggcg atcgtaggcc
      661 ggccaagaaa gaggttttgt cgctggccgg gccggcgtat gatgccggga ccatgagcga
      721 gttcgacctc ggagccatga tgcggcaggc gcaacagttg cgcgagcaga tggagaccat
      781 gcagaagagc ctcgacaagg agatcgtcga gggcaccgcc gggggcggca tggtcaaggc
      841 gaaggccacc ggcacgcagc agatcctcag catcgagctc gccccggcgg ctctgcaaga
      901 ggagcgcgag atgctgcagg acctgatcgt cgccgcggtc aacaacgccc tcgagcgctc
      961 caaggcgctg acccagcaaa aggtcggctc gctgctgccg ccggggctga tggggccggg
     1021 cggcatcccc gggctgtgag cggcggcggc gacacacaga acccgctgca gcggctggcg
     1081 gcgctgctga cccgtctacc gggcgtcggc gagaagagtg cgttgcggct ggcgctggcg
     1141 ctggtgcgtt cggacagcga gtacgtgcgt gcgctgtcgg aggcgatcgg ggcgatccac
     1201 gccgagctgc gactgtgcgc gacctgttgc gacctctgca ccgcggagat ctgcgagcgc
     1261 tgtcgggacc cgcgacgcga ccccagcctc gtctgcgtgg tcgcgcagcc gcaggaccgg
     1321 atggcgatcg agcggaccgg cgcttattcg gggctttatc acgtgctgca cggggtcctc
     1381 gacccgctcg cgggcatcgg ccccgccgag ctgaagatcg acgccctgct gcgccggctg
     1441 cgaggcgacg cggtgcgcga ggtcatcgtc gccaccagcc ccaacgtcga gggcgacgcc
     1501 acggccctgt acctgtcgaa gctgctgcag ccgctcgggg tccgggtcac gcggatcgcc
     1561 tccggggtcg cggtcggcgg ggagctcgag tatgcggatc tgaacacgct gcatcgcgcc
     1621 ctggcggagc gccgctcgct gtgagggttc ctcgcgagcg ggccgtgatg cacggtccag
     1681 ggcaggtcat cggcggtttc gccggtccgg gcgcggccga cgcggcgctg gttccccgtg
     1741 atggacctgt cgcgcacgaa aattcagccc caaagcaccg gggagatggt cgcgccacgc
     1801 cacacgggcc acaaaaacgc ggggcggtcg ggccgaaccg tggttctcgg cgcgatggac
     1861 ttgccgggcc tagtttgtta cggttttcac gccgtcgggc atcggtccga agttcacacg
     1921 tcgcgcatca ggagtctgtc ccttgaaccc gatgaatccc cagctcgtga tgtttgaaga
     1981 ggagctcaag cagatccaaa agatcgttga ccggctcctg tcggattcac gatcgcgatc
     2041 gatccttctc atcgacaaga acgggcagct cgtcgccgcc tctggcagcg ctcatgagct
     2101 cgacacgacc tcgctcagct cgctggtcgc cggcaacgtc gcggccaccg gtggcatcgc
     2161 caagctgctg caggaagacg agttcaccgg tcagttccac gagggcaagg ggatgtcgat
     2221 caacctgtcc ctcgtgggcc ggcgcatcat cctcgtcgtg ctgttcgaca agaacaccac
     2281 gcccgggctg gtccgtctcc gggtcaagcg ggcgcaagat gaactggtcc cgatctttga
     2341 ggcgctggcc acgaaggctg cacagcccaa cggtggctcg ccggcgctgc aagagatcac
     2401 cgaccaggat atcgacgacc tgttcaacga ctagtagagc gagacacccc accgatgtcc
     2461 ttcatcaact acagctcgcg tgagatcaac tgcaagctgg tctattacgg gccgggtctg
     2521 tgcggcaaga ccacgaacct gcagtacatc tacaacaaga cgcgacccga ggcgaagggc
     2581 aagatgatct ctctcgccac ggagaccgag cggacgctgt tcttcgactt cctcccgctc
     2641 agcctcggag agatccgcgg cttcaagacg cgcttccacc tctacaccgt ccccggccag
     2701 gtcttctacg acgccagccg caagctgatc ctcaagggtg tcgacggcgt ctgcttcgtc
     2761 gccgactcac aagaggagcg ctacgacgcc aatatcgaga gcctcgagaa cctccggctc
     2821 aacctgcaag agcagggcta cgacctcgac aagctcccct acgtcgtcca gtacaacaag
     2881 cgcgacctgc ccaacgcgat gagcgtcgcg gagctctcga agatgctcaa cccgacgcag
     2941 gtccccgagt tcgagggcgt cgcaacgacc ggcatcggcg tgttcgatac gctcaagatg
     3001 ctcgccaagc tggtcctcgc cgagttgcgc aagggcacct gaaggcgccg ctcgtcgcgt
     3061 gcctccgatg tggcacctgc gtatgagtca ggctctttaa aagagatgaa aatcacgcct
     3121 cgcagcgagg cctcgcgtat cgttgaacgg caaaccagtc cggagcgacg cggtcatatg
     3181 accgcatcag ctgaccggct gcgcatgcac gcgcgtcttc caccggagat gatgcgatgt
     3241 cgcatgcagc ggcacacacg ccggacacgc tgcacgtcgc ggagatcgac cagcgtcggg
     3301 gcacgttcgg gaattaatgg ttggagcgtc ttgaacgctg tgtattatgc ctgtcgagac
     3361 gcgactcgcg ctctttgagc gcagaacttg agcggagcct cagcacctat gcccacggaa
     3421 gactggattc aggaatccct cgcgcggctc gagagcctgg aagaagagcg caaccggcac
     3481 gaagccgcgc tggagacctc gacggatccc gccgcgctgc ggatgaacgc acaggcgatc
     3541 gaacgactcg acgcgaagat ccgctcgctc tacgcagagc tcgaggccgc cgccgaacaa
     3601 ggcgacgccg aacagggtga cgctgtcgac accgacctcg gcgatgaccc cgatctcggt
     3661 gacgaggccg agggcgagga cgacgtcgcc ggcggtacga cgcagatgtt ccgccgcgac
     3721 gacctcgccg cccaggccgc cgccatgcgc acctccccgg agaccgtccg gtcagtgccc
     3781 gccagcccga agatcgggcc ggccgccgct gctgcgcccg ccgccgctgc gagggtcacc
     3841 ccggtcgccg ccgctgccgt cgctgccgcc actgcaccga ccgcctcgcc tttttcggct
     3901 ccggtcagca cgcccgcgcc cgtgagcagc gcacccgcag cgtcgccctt cggcggtgcc
     3961 gctgccgcca gcgctccgac ggcctcgccc ttcggcaact ccgcgtcgcc cttcggcgag
     4021 gtgccccagg cggcgccctt cggtaactcc ggagattcgg ccggcttctc cgcgccggcg
     4081 acctcgaact cctccttcga cgcgggcaac agcttcgatg acgagtcgag ctccggctcg
     4141 ggttcgaaga tggggatcat catcggggtc gtcgtggtgg tgctcggcct cggtgcctac
     4201 ctggcgttcg gcggcaaggg cgacgagccc ccgcccgcgg cgccggccgg tcccgtcaag
     4261 gtcatcggcg gcctggaagt ccccccggac acccagggcc ccaagaccgt ccagggcagc
     4321 agcgacgcga tcaaaggcac gcagtacaag gaaggccgcc cgcaatccgg cggcggcggt
     4381 ggtggcagca acgccccgcg ccccagcgac aagccggtca agaaggaccc gcgcgccaag
     4441 atccagaacg ccgacgaccc gctggccggc atcgacaagt aaaagatggc tgtcct
//
LOCUS       DASWNJ010001998         4193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_418800, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001998 DASWNJ010000000
VERSION     DASWNJ010001998.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418800"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VGB85_33320"
     CDS             complement(<1..640)
                     /locus_tag="VGB85_33320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HEY0139031.1"
                     /translation="MFRKSDTHIHFVGIGGIGMSGIAEVLLTLGYQVTGTDLAESETT
                     RRLTELGARVHVGHAAEFLGHADVVVVSSAVRPDNPEVAAARERHIPVIPRAEMLAEL
                     MRLKQGIVIAGSHGKTTTTSLIASVLQRAGLDPTVVIGGKLNSLGSSARLGSSDILVA
                     EADESDGSFLVLTPTIAVVTNIDDEHMDHYGTMARLSDAFVGFANRVPFYGMA"
     gene            698..1858
                     /locus_tag="VGB85_33325"
     CDS             698..1858
                     /locus_tag="VGB85_33325"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF026165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEY0139032.1"
                     /translation="MTDFARGPDGPQRPPQATEATETARADRLARALGPWFVQHQRDL
                     EWRRTRDPYAIWVSEVMLQQTRVDTVKAYWTPFMQRFPDIATLAAADEQQVLAAWSGL
                     GYYRRARLLHQGARHVHTTMAGTLPTRPDELRQIPGIGRYTAGAIASIAHDAPHALVD
                     GNVARVLSRLQAITEPKLQLPDAAGHWTMAQRILEAGSPRVLAQALMELGATVCTPQL
                     PRCGECPVRGDCLAFARDLVATIPAPKKKAPSPQQDLWALALQRQGKLLLVRRPAEGL
                     LAGMWTLPLLDRPAAAPEAPHGSLFDRARPTPAAIRKLTGLRVSKPEALRVPVKHVFT
                     HRVWILWPVRAAVDLSDGSGSPPDEHAWIAPGERPVGGIPTITEKLLEPLGY"
     gene            complement(1890..2126)
                     /locus_tag="VGB85_33330"
     CDS             complement(1890..2126)
                     /locus_tag="VGB85_33330"
                     /inference="COORDINATES: protein motif:HMM:NF024251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HEY0139033.1"
                     /translation="MELVPDPQVLLELIRARMPFGKYEGWRLTALPEPYLLYFRRQGF
                     PEGKLGRQMALALEIKTNGLEPLIRELAQQTGEQ"
     gene            complement(2141..2797)
                     /locus_tag="VGB85_33335"
     CDS             complement(2141..2797)
                     /locus_tag="VGB85_33335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139034.1"
                     /translation="MKPGPVRPVLVRLSLLGALALVACTEKGGTTESGASTGESSSGS
                     TSTATTGSSDPGTTGGIATVTSETNTTQDPECGPNTCGPCPEDCTAQDACVGGEWQCE
                     CACASTGASDSSSTGTPAMGCAADPPQFPEFDRSCADDSECTIVFHQVDCCGTLVAWG
                     LSSAVGKMFGEAETQCKMEYGECECAAQPTTADDGKTAEDTTTIVVACVDGVCSSSVP
                     "
     gene            complement(2914..3678)
                     /locus_tag="VGB85_33340"
     CDS             complement(2914..3678)
                     /locus_tag="VGB85_33340"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEY0139035.1"
                     /translation="MKPVTTQDLRPIGTISLAIAMVAATFIAASTWERVKVKPEARSI
                     EITGSAKKRIVSDLIEWSASIETSAPDRMAAYKQLHDHASRAQEFLTGRGVAAKEIWA
                     SSVSVDAIYETEYIGTGEDRIARQKLQGYMARQSVVVRSADVPLVEKMSREITELLEQ
                     GITITSSAPLYHYTKLGEMKIEMLAEASKDARTRAENMVASAGGATLGKLVSADMGII
                     NINPANSTQGSWDGNNDTTSLEKDIITIIHATYELE"
     gene            complement(3781..>4193)
                     /locus_tag="VGB85_33345"
     CDS             complement(3781..>4193)
                     /locus_tag="VGB85_33345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139036.1"
                     /translation="IFSTTEAEARRNELKVQIGDLRGELAQQRGQLAATFWEALRGYP
                     PALLRAELDTVVQAVENIRAVCRSYTVTVGSGKNRRTERRYRCELVGHDAAVTAMTRW
                     SALMVKVFGEQLGYHELLEAVELEPAGGEEPPQS"
BASE COUNT          585 a         1584 c         1371 g          653 t
ORIGIN      
        1 ccgccatgcc gtagaacggc acgcggttgg cgaagccgac gaacgcgtcg ctgaggcgcg
       61 ccatggtccc gtagtggtcc atgtgttcgt cgtcgatgtt ggtgacgacc gcgatcgtcg
      121 gcgtcagcac gaggaagctg ccgtcgctct catcggcctc ggcgaccaaa atatcggagg
      181 acccgaggcg cgcgctcgac ccgagcgagt tgagcttgcc gccgatcacc accgtcgggt
      241 cgagcccggc ccgctgcaac accgacgcga tcaggctcgt cgtcgtcgtc ttgccgtgcg
      301 acccggcgat cacgatcccc tgcttgaggc gcatcagctc ggcgagcatc tcggcgcgcg
      361 ggatcaccgg gatgtggcgc tcgcgggccg ccgcgacctc gggattatcc gggcgcaccg
      421 ccgacgacac caccaccacg tccgcgtgcc cgaggaactc cgccgcgtgc ccgacatgca
      481 ccctggcccc cagctccgtc agccgccgcg tcgtctccga ctccgcgagg tcggtgccgg
      541 tcacctgata cccgagcgtc agcagcacct cggcgatgcc gctcataccg atgccgccga
      601 tgccgacgaa gtggatgtgg gtgtcgctct tgcggaacat gcggggccgc gagcttactc
      661 ctgctgcccg gccgcgaaaa cgtgctacat cgcggccgtg accgatttcg cgcgcggccc
      721 ggatggccca cagcggcccc cacaggccac cgaagcgacc gagacggccc gcgccgatcg
      781 gctggcccgc gcgctcggcc cgtggttcgt ccagcaccag cgagacctcg agtggcgccg
      841 gacccgcgac ccgtacgcga tctgggtctc ggaggtgatg ctgcaacaga cccgcgtcga
      901 caccgtgaag gcctactgga cgcccttcat gcagaggttc cccgacatcg cgaccctcgc
      961 cgccgccgac gagcagcagg tgctcgccgc gtggagcggc ctcggttact accgccgcgc
     1021 ccgcctgctg catcagggcg cccgccacgt gcacaccacg atggccggca ccctgccgac
     1081 ccggcctgac gagctgcgcc agatccccgg catcggtcgc tacaccgccg gcgcgatcgc
     1141 ctcgatcgcc cacgatgccc cgcatgcgct cgtcgacggc aacgtcgcgc gggtgctctc
     1201 gcgtctgcag gcgatcacgg agcccaagct gcaacttccg gacgcggccg ggcactggac
     1261 gatggcgcag cggatcctcg aggccggctc gccgcgggtg ctcgcgcagg cgctgatgga
     1321 gctcggcgcg accgtctgca ccccgcagct gccgcgctgc ggcgagtgcc cggtgcgcgg
     1381 cgactgcctc gcgttcgccc gggacctcgt cgccacgatc cctgccccca agaagaaggc
     1441 cccgtcgccg cagcaggacc tgtgggcgct cgcactacag cgccagggca agctgctgct
     1501 cgtccgccgc cccgccgagg gcctgctcgc cggcatgtgg accctgccgc tgctcgatcg
     1561 ccccgccgcc gcgccggagg ccccgcacgg cagcctcttc gaccgcgccc gcccgacccc
     1621 cgccgcgatc cgcaagctga ccggcctgcg cgtcagcaaa cccgaggccc tgcgcgtgcc
     1681 ggtcaagcac gtgttcaccc accgcgtgtg gatcctctgg ccggtgcgcg ccgcggtcga
     1741 cctgtccgat gggtcaggta gcccgcccga cgaacacgcg tggatcgccc ccggcgagcg
     1801 cccggtcggc ggcatcccga cgatcaccga aaagctgctc gagccgctcg gctactgacc
     1861 accgatccac cgatccaccg atccactgat cattgctcgc cggtctgctg cgccagctcg
     1921 cggatcagcg gctcgaggcc gttggtcttg atctcgagcg cgagcgccat ctgccggccg
     1981 agcttgccct ccgggaagcc ctggcggcga aagtacagca ggtacggctc gggcagcgcg
     2041 gtcaggcgcc agccctcgta cttgccgaag ggcatgcgcg cccggatcag ctcgagcagg
     2101 acctgcggat cggggacgag ctccaccgcg gcagtctacc tcacggcacc gagctgctgc
     2161 acacgccgtc gacgcaggcg accacgatcg tggtcgtgtc ctcggcggtc ttgccgtcgt
     2221 cggcggtcgt cggctgcgcc gcgcactcgc actctccgta ctccatcttg cactgcgtct
     2281 ccgcctcgcc gaacatcttg ccgaccgcgc tgctcaggcc ccacgcgacg agggtcccgc
     2341 agcagtcgac ctgatgaaac acgatcgtgc actcgctgtc gtccgcgcag ctgcggtcga
     2401 actccgggaa ctgcggcggg tcggcggcgc agcccatcgc cggggtcccc gtgctcgacg
     2461 agtccgaggc cccggtcgac gcgcacgcac actcgcactg ccactcgccg ccgacgcacg
     2521 cgtcctgcgc cgtgcagtcc tccggacacg ggccgcaggt gttcgggccg cactcggggt
     2581 cctgcgtggt gttggtctcg ctggtgacgg tggcgatgcc gccggtggtg ccggggtcgc
     2641 tgctaccggt ggtcgccgtc gacgtgctgc ccgagctgga ctcgccggtg gaggcgccgc
     2701 tctcggtcgt gccgcccttc tccgtacacg cgacgagggc gagcgcgccg aggaggctca
     2761 ggcggacgag gacaggacgg acggggccgg gcttcatgac caccagtgta acccgagtcc
     2821 gcgatcgcgc agccgcccgg cccgcggtga cgacgatctt tgcgagggag acacctacac
     2881 cggccaccgt ccggccaccc cggccacccc ggctcactcg agctcgtagg tcgcgtggat
     2941 gatcgtgatg atgtccttct cgagcgaggt cgtgtcgttg ttgccgtccc acgagccctg
     3001 cgtcgagttg gccgggttga tgttgatgat gcccatgtcg gcgctgacga gcttgccgag
     3061 cgtcgcgcct ccggcagagg cgaccatgtt ctcggcgcgc gtgcgggcgt ccttcgacgc
     3121 ctccgcgagc atctcgatct tcatctcgcc gagcttggtg tagtgataga gcggtgccga
     3181 cgaggtgatc gtgatgccct gctcgagcag ctcggtgatc tcgcgcgaca tcttctccac
     3241 cagcggcacg tcggccgagc gcacgaccac cgactgccgc gccatgtagc cctggagctt
     3301 ctgccgggcg atgcggtcct cgccggtgcc gatgtactcg gtctcgtaga tcgcgtcgac
     3361 cgagaccgac gaggcccaga tctccttcgc cgccacgccg cggccggtca ggaactcctg
     3421 cgcgcgcgag gcgtgatcgt gcaattgctt gtaggccgcc atgcgatccg gcgcgctcgt
     3481 ctcgatgctc gccgaccact cgatcaggtc ggagacgatg cgcttcttcg ccgagcccgt
     3541 gatctcgatg ctgcgcgcct cgggcttgac cttgacgcgc tcccacgtgc tcgccgcaat
     3601 aaaggtcgcg gcgaccatgg cgatcgccag gctgatcgtt ccgatcgggc gcaggtcctg
     3661 ggtagtgacg ggcttcaaat attccacgtg gctgtctccg gtccccgcgt acgcggccga
     3721 cggcccggac attccgggcc cccgaggttt ttgcaagatc cgcggtcctt cgcaagaccc
     3781 tcagctctgc gggggctcct cgcccccggc cggctcgagc tccaccgcct cgagcagctc
     3841 gtgatacccg agctgctcgc cgaacacctt gaccatcaac gccgaccacc gcgtcatcgc
     3901 cgtcaccgcc gcgtcgtgac cgaccagctc gcagcggtag cgccgttccg tgcgccggtt
     3961 cttgccgctg ccgaccgtca ccgtgtacga gcgacacacc gcgcgaatat tttcgaccgc
     4021 ctgcaccacc gtgtcgagct cggcccgcag caacgccggc ggatagccgc gcagcgcctc
     4081 ccagaaggtc gccgcgagct gcccacgctg ctgggccagc tcgccgcgca ggtcgccgat
     4141 ctgcaccttg agctcgttgc ggcgcgcctc cgcctcggtc gtgctgaaga tgt
//
LOCUS       DASWNJ010001999         3711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_387829, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010001999 DASWNJ010000000
VERSION     DASWNJ010001999.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387829"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VGB85_33350"
     CDS             <1..276
                     /locus_tag="VGB85_33350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139037.1"
                     /translation="TVRREAVTEAEALKAAQRAVPSLRTCADVPRHVTADLDIVRGRG
                     IVTALNLHAPDLDDPKSWHGCVVRSLKPVRFPVSQTAGHVRVRLTLR"
     gene            390..629
                     /locus_tag="VGB85_33355"
     CDS             390..629
                     /locus_tag="VGB85_33355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139038.1"
                     /translation="MASFYRNVLPDFTGLENGTSEVNVVERDGSIGLSMTQGHGEGWS
                     CAQVFMTPEQGHELLRGLLEAIDRAERQRSLRRPN"
     gene            1612..3057
                     /locus_tag="VGB85_33360"
     CDS             1612..3057
                     /locus_tag="VGB85_33360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139039.1"
                     /translation="MTLTNQGSLLSLALVLGCRAPAPSAQPAAPPKQQTVLASSPTIS
                     SPVAAGPQSGPQDGVVPPQDAIAQPAAVQCVGSRPRTPPEWRDIFVSFAKNSTRPTSS
                     FDSTTSSRILMLLREHPDLALWIQGERLYPGERTSLIRPRLAAVRRQLLRLGVDPDQV
                     LIDDGQPIGHVVVVSMRARPPAPVKPGEPCGTTVLRTSRATVVRDLAALGWWARKALS
                     SDDNFRDVRTHRGTQLEPMQGSRLTPLVRRPGGYATVRWNPLLDAFEFTLLDIIGGPQ
                     QTRRLPGPASTGVDHLALVGHPGGFILAWVQGFDELHTAALDLSGDPVRASGPELLAG
                     APIDALTTDWRDGAGGIAYTLGNPSDPFGPLMYAAIDAQGHFQGAPRMLFDGVVAPGP
                     LAMATLGPDRVSVLVVACPPFTMRIDVDARGDMVAVDEEFLRFPSWLGLGFVRGPNGV
                     WAASIGESMVDLRPMCEVPGGSGTRAVIDKP"
BASE COUNT          599 a         1293 c         1200 g          619 t
ORIGIN      
        1 accgtccgcc gtgaagctgt caccgaggcc gaagcattga aggcggcaca gcgcgccgtg
       61 ccaagcctcc gcacgtgtgc cgacgtccca cgccacgtga ccgcggacct cgacatcgtt
      121 cgcggccgcg gcatcgtcac ggcgctcaac ctccacgccc cggatcttga cgatccgaag
      181 tcctggcacg gttgcgtggt gcggagtctt aagcccgtcc gcttccccgt cagccagaca
      241 gccggacacg tccgggtccg gctgaccctg cggtagaccg tacggccagc atcgcgcaag
      301 cggccgaagc ggctagcccg gcgcagccac gctgcaagta aaacacccag ttccctcgcg
      361 tgtggcgacc tcgcatgctg gggaccgata tggcgagctt ctacaggaac gtgctcccag
      421 acttcacggg cctcgagaac gggacaagcg aggtcaacgt cgtcgagcgg gacggcagca
      481 tcgggctctc gatgacacaa gggcatgggg aaggctggag ctgtgcgcag gtcttcatga
      541 cgcccgagca gggccatgag cttcttcgcg gactgctgga agccatcgat cgcgcggaga
      601 gacagcgctc tctacgacga ccaaactgac aggtgcggcc tgaagcggtg gcggggcgcg
      661 gggtcacgca atctggcccc ggggacaggg ccgccgggca ccgagacagg ggccgaagtc
      721 gaccggatcc ggccccgcgg ctgtcgaccc tgccccccgt acgagccccg gcaggggcac
      781 atgcgcggcg gcgagatccc cgggtctcgg cccgatcctg tcccgatttt gtcgcgattc
      841 gggtctgtct cgagacaaac ccccgaattc gggctcctag ggccccctac gcgaccacac
      901 tcaccacatc accaatatct cgtcggatcg ccctcccaag ttgcaagcta cccggcgaaa
      961 tccttgtttc aactgaaatg acatttcaga tccctcggca ggttcggccg caactggccg
     1021 ctcttggcgc cgctaaacgt tggtctgcgg ctcaaacttc cggagacagg gcgccaaaac
     1081 tgacttcgac aaccgggcgt ctttcggaga atcaggcgcg acggggccaa cgttagccga
     1141 cggctggtga ttgcaggtcc accgcacctc ggagatccac gcgcccgggc gacgcatgca
     1201 cgcgagggca tcagcctcgg cgacgacgcg agcctcgtcg ctgtccgcgg ccgcccggag
     1261 ggtcacggat gcttcacgtg tgtatgaaca tacacggacg actttgccgc gcccggcagg
     1321 tgccaggcgc cgcaggtcgt ccacgccgcg ggtggctgct cccgcgcgaa tcggcttctc
     1381 gctcgagcgg atcggaggcg ctggatacct gcaatttggc cgacaaactc ccatatggcg
     1441 acctgcaggt ccggcacgcc gtaggccctc cgcggctgga cttggctcgc agaccgccga
     1501 cgtgccagtg catgtggggg ccgaggggtt ggacgaagcc aatgcccttg gtattgtgtt
     1561 cgggatggac gggcgaggcg tcacgcggca tgacctatcg agcggctcac gatgacactc
     1621 accaaccagg gcagtctgct gtcactcgcg ctcgtcctcg gctgtcgggc tccggcgccg
     1681 tccgcacagc cagccgcgcc accgaaacag caaaccgtcc tggcgtcctc gccgacgatt
     1741 tcctcgccgg tcgcggccgg gccgcagtca ggtccgcagg acggagtggt gcctccgcag
     1801 gacgccatcg cacagccggc cgcggtgcaa tgcgtcggta gtaggccgcg aactccgccg
     1861 gagtggcgcg acatcttcgt atcgttcgcc aagaacagca cgcgaccgac ctcgtcgttc
     1921 gactcgacga catcgtcgcg gatcctgatg ctgctgcgcg agcacccgga tctggcgctg
     1981 tggatccagg gcgaacgcct gtatcctgga gagcggacct cgctgatccg tccgcggctc
     2041 gcggcggtcc ggcgtcaact cctccggctc ggcgtggacc cggatcaggt cttgatcgac
     2101 gacggtcagc cgatcggcca cgtcgtggtc gtctcgatgc gcgcgcggcc cccggctccg
     2161 gtgaagccgg gagagccatg cggcacgact gtccttcgga catccagggc gaccgtcgtc
     2221 cgcgacttgg cggccctcgg ctggtgggcc cgtaaggcgt tgtcgtccga cgacaacttt
     2281 cgcgacgtcc gcacacaccg gggcacgcag ctcgagccga tgcaggggtc cagactcacc
     2341 cccctcgtcc gccggccagg cggttacgcg acggtacgct ggaaccctct tttagatgcc
     2401 ttcgagttca cgttgctcga catcatcggc ggcccgcagc aaacccggcg gttgccgggc
     2461 cccgcatcga ctggcgtcga ccacctcgcg ctcgtagggc acccgggagg gttcatcctc
     2521 gcgtgggtac aggggttcga cgagctccat accgccgctc tggacctctc cggcgaccct
     2581 gtacgggcgt ccggacccga gctcctggcc ggggccccga tcgatgcgct gaccacggac
     2641 tggcgcgatg gcgccggcgg tatcgcctat accctcggca atccgagtga ccccttcggt
     2701 ccgctgatgt acgctgcgat cgacgcgcag ggccacttcc agggcgctcc gaggatgctc
     2761 ttcgatggtg tggtcgcgcc cggtccgctc gccatggcca cgctcggacc ggatcgagtg
     2821 agcgtcctgg tggttgcatg cccgcctttc acgatgcgaa tcgatgtcga cgccagggga
     2881 gatatggtag ccgtggacga ggagttcctc cgcttccctt cgtggctcgg gctggggttc
     2941 gtgcgcggcc cgaacggcgt atgggcagcg tcgatcggcg agtccatggt cgacctgcgc
     3001 ccgatgtgcg aagtgccggg cggctcaggt actcgtgcgg tgattgataa accctagccc
     3061 gtcggctccg atgaattgct caggtatcag ggcatcttct tcaggtacgg cagccaggac
     3121 atcgaggagt gatcttcact cgcggcgtac gagaatcgcg gtctcgtgca tgcagggccg
     3181 cgaacacgcg gcatcgcccg tccctgcaca gcagggttgc cccatgaagc gggacagcgc
     3241 cggtgcgagc tctgcgagcg tcatcgccgg tcacggcacc tcgccgaccg cccgcgcgac
     3301 ccccggggac cctccgacca tgccccgacc gtcgggtgca tctttcttgg cctcgctcat
     3361 cgccgggcgg ggtcctcgat cgccatgtcg gatcgtctcg ccccgctcat cgtccgccgc
     3421 atccccagca ccgcgatcga cggggtatgc ggcgacagtt gacctgtcca gaaagacagg
     3481 tctgacgggc accgagacag cgttcaaagt cgacccgctc cggccctgtg gctgtcgacc
     3541 gtgccctgcc tgccagtccc cggacggcca catgcgcggc cgcgagctcc ctgggttctg
     3601 gcatgatctt gtcccgattt tgtcgcgatc cggtcctgtc tcgagacgaa ctcccgaatt
     3661 cgggctcgca gggctcccta cgcgaccaca cgcatcacat caccaacgtc t
//
LOCUS       DASWNJ010002000         1581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_176178, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002000 DASWNJ010000000
VERSION     DASWNJ010002000.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176178"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..>1581)
                     /locus_tag="VGB85_33365"
     CDS             complement(134..>1581)
                     /locus_tag="VGB85_33365"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY0139040.1"
                     /translation="RDHVTTAELAFDENKRIIGMRVDLIANMGAYYYFYAPYIPTGAA
                     LKVLPGVYAIPCLSYGVKGVFTNTVPIDAYRGAGRPESIYCVERLIEAAARELKVDPT
                     ELRRINFVRPEQMPFDTAAGETYDSGDFARVMDTAKQRADWDGAAARRASARARGKRL
                     GIGMCYYIESTMGDPTEHAAIRFEEDGTVAVLVGTQTNGQGHDTAYAQVLHQRLGVPF
                     ERIRVVQGDTDQIRAGGGTGGSRSLTAQGMAIMDASDVVEQRGKAYAAQVFEAAQADI
                     TFDAGEFRVAGTDRAIDIMELAGRSRAVTVAGLEGAGLDAAAMTKLSAWTFPNGCHIA
                     EVEVDPDTGIVDIARYTVVDDFGVVVNPMLVEGQVHGGIVQGLGQALLEGAVYDDAGQ
                     LLTGSFMDYAMPRADNMPSFDFSTVEVPCKNNALGVKGCGEAGSVGSPAAVINAIIDA
                     LADLGVRRVDMPATPQRLWELMHQGRVAAAA"
BASE COUNT          206 a          565 c          567 g          243 t
ORIGIN      
        1 ccacctcgcg gccgagctgg cacatcgtct gtcgagttag gaggacccca tcgcgcccgc
       61 ttcggcgatc gcggctcgcg cggggacgtc gggcaccggc cgcggaccgg ccggtgcccg
      121 atgtccttcc agttcaggcc gcggccgcta cccgcccctg gtgcatcagc tcccacaggc
      181 gctgcggggt cgccggcatg tcgacgcgcc gcacgccgag gtccgccagc gcgtcgatga
      241 tcgcgttgat caccgccgcc ggcgagccca cggagccggc ctcgccgcaa cccttcaccc
      301 ccagcgcgtt gttcttgcag ggcacctcga ccgtcgagaa gtcgaacgag ggcatgttgt
      361 cggcgcgcgg catggcgtag tccatgaacg agccggtcag cagctggccg gcgtcgtcgt
      421 agaccgcgcc ctcgagcagc gcctgcccga gaccctgcac gatgccgccg tggacctgcc
      481 cctcgaccag catcgggttc accaccacgc cgaagtcgtc gacgacggtg tagcgggcga
      541 tgtcgacgat gccggtgtcg ggatcgacct cgacctcggc gatgtggcag ccgttcggga
      601 aggtccaggc gctcagcttg gtcatcgccg cggcgtcgag gcccgcaccc tccaggccgg
      661 cgacggtcac ggcgcgtgac cgcccggcca gctccatgat gtcgatggcg cggtcggtgc
      721 cggcgacgcg gaactcgccg gcgtcgaagg tgatgtcggc ctgggccgcc tcgaacacct
      781 gggcggcgta ggccttgccg cgctgctcca ccacgtccga ggcgtccatg atcgccatgc
      841 cctgggcggt cagcgagcgc gagccgccgg tgccgccgcc ggccctgatc tggtcggtgt
      901 cgccctggac gacgcggatg cgctcgaacg gcacgcccag ccgctggtgc aggacctggg
      961 cgtaggcggt gtcgtggccc tggccgttgg tctgggtgcc gaccagcacg gcgaccgtgc
     1021 cgtcctcctc gaagcggatc gcggcgtgct cggtcgggtc gcccatggtc gactcgatgt
     1081 agtagcacat gccgatgccg agccgcttgc cgcgcgcccg cgccgaggcc ctgcgtgccg
     1141 ccgccccgtc ccagtcggcg cgctgcttgg ccgtgtccat cacccgcgcg aagtcgccgg
     1201 agtcgtaggt ctcgccggca gcggtgtcga acggcatctg ctcaggccgg acgaagttga
     1261 tgcggcgcag ctcggtcggg tcgaccttga gctcgcgcgc cgcggcctcg atgagccgct
     1321 cgacgcagta gatcgactcc ggccggccgg cgccgcggta ggcgtcgatg ggcaccgtgt
     1381 tggtgaacac gcccttcacg ccgtagctga ggcaggggat ggcgtagacg ccgggcagca
     1441 ccttcagcgc cgccccggtc gggatgtagg gcgcgtagaa gtagtagtag gcgcccatgt
     1501 tcgcgatcag gtcgacccgc atcccgatga tgcgcttgtt ctcgtcgaac gcgagctcgg
     1561 cggtggtgac gtggtcgcgg c
//
LOCUS       DASWNJ010002001         3683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_141033, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002001 DASWNJ010000000
VERSION     DASWNJ010002001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141033"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1015
                     /locus_tag="VGB85_33370"
     CDS             116..1015
                     /locus_tag="VGB85_33370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139041.1"
                     /translation="MPAFVSVLALALAAPGPAAPPLSPTADNDALHTEIAAGDPVLTA
                     PAPPASLERLVAATEIAEEKLRQAADDDDAEDLLTLTAQGRRAAYGRTGEALHLCRLI
                     AAADVVLARAAVPPRLVAAATDFRRESQDSLGATPCEDTAPPEKTDDPPPDRAAVDRS
                     AAAVGPSEPPPPRPVVQPAAPVDRRRVRVGVGTLVPGLVLLAPMVGLLAYRAAGERDL
                     TALDRETMARPRTAADDAAAAALGQRYTTTTAGAAVLGVTAAALVVTGAVFLATPGKR
                     QRRMAVAPWGTWGSGGLVLRGKF"
     gene            967..2763
                     /locus_tag="VGB85_33375"
     CDS             967..2763
                     /locus_tag="VGB85_33375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139042.1"
                     /translation="MGNLGKRRTGAAGEILTMREIAFSGKMAVMFRRSLALLTLLAGC
                     TSPAYELYLEHADDAGSTVASSDGAAETTGDATPTSTGGTGSDSADATGDTSDATDTA
                     PLSDSATDSATDTGDATDTEDSPPVGEADKPVIVSIELPANVYTAGPVPLAVQTEGTA
                     AVHVTLNGADAGELIAAGGGLFTGALPVHGAIDNGSHAVEVIATQGKYEDRKPAMFNV
                     STPEPGTEAWSLPGPDGSRTNRVALTPAGDLIEVGQTVIGGVPRPTIRKRSSVTGAEL
                     WPEGTITLDTREGAVVDVAALPDGRMWVAMNVRPPGKDQRPRIVLLDADGHATGVGLE
                     GTPGRVVRAIAADADGGCFAVGLAVVMGDWDIAYWRIDAANEPTLGHAFDYWPPKHPE
                     HSFMDLASDVVIDGDVAWVVGMSKGKHDAEQKDPDLYLRGVLVPLNLHTGKLAGSVLV
                     APAAPGWSQSALFGAALHPAGVLATGYGCDKTCSTYRIETSLYSVDGVRTWFEHEAKN
                     DGLAYGSDVVLDGQGRALVSAAVTQNGKLRGYVFGRKVGLPGPVLLEHWYPGAGPSEA
                     LGIARDVFDRIFPVGYITFNGETQARVTRIHG"
     gene            complement(2794..>3683)
                     /locus_tag="VGB85_33380"
     CDS             complement(2794..>3683)
                     /locus_tag="VGB85_33380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139043.1"
                     /translation="VMDFGPAQPPGCKGKVDLLFVISRQGTMVTEQEQLLASFPGFIE
                     TIEQTLEGFDVHIMTANPDAYWPGWTCQNQPDGCENVENWPTCGPNAPDYNCQTFPDM
                     MTPCDEQLGAGLIFNAGGDAANKLCKLHEGRRYIVSGEPDMPGAFECIAKVGVGGKAA
                     LMGDAMIAALSPELNGPGGCNEGFLRKDALLVVALITDFHDQGKSYPKQQYEAIIAAK
                     GDPSAVVMLAVVPNAPGEFEPAEDCHYWDEPSFGIFEDLLSRFPYAVFGDTCTPSFAP
                     FFEQAADKISEACGSFIPQ"
BASE COUNT          543 a         1319 c         1292 g          529 t
ORIGIN      
        1 gacggggtcg gagtgctgtc catcgtcgcc aagcctgcca caaccctgcg ccgagaggtc
       61 cccccgcgtg ccctgggatc ttccagcctc cccgccagtg cgatacaaga agaccatgcc
      121 cgccttcgtc tctgtgctcg ccttggccct cgcggcgccc ggccctgccg cgccgccgct
      181 gtcgcccacc gcggacaacg atgcgcttca caccgagatc gccgctggcg atccggtgct
      241 cacggccccg gctccgcccg catcgctcga gcggctcgtc gccgccacgg agatcgcgga
      301 ggagaagctg cgccaggccg ccgacgacga cgacgcggaa gacctgctga cgctcacggc
      361 gcagggacgc agggccgcct acgggcgcac gggtgaggcg ttgcacctgt gccggctgat
      421 cgccgctgcg gacgtcgtgc tcgcgcgcgc ggcggtgccg ccgcgtctcg tagctgccgc
      481 gaccgatttt cggcgagaat cgcaggacag tctcggtgcg acgccttgcg aggacacggc
      541 cccgccagag aagacggacg acccgccgcc ggaccgggcc gccgtggacc gtagcgcggc
      601 cgccgtgggg ccgtccgagc cgccgccccc gcggcccgtc gtccagcccg ccgcccccgt
      661 cgatcgtcgc cgtgtccgcg tgggcgtggg gacgcttgtc cccggcctcg tgctgctggc
      721 acccatggtc gggctgctgg cctaccgcgc cgcgggcgag cgcgacctga cagccctcga
      781 ccgcgagacg atggcgcggc ccaggacggc cgcggatgat gccgccgccg cggccctcgg
      841 ccagcgctac acgacgacga ccgccggggc ggcggtactc ggggtcactg ccgctgcgct
      901 cgtcgtgact ggcgcggtgt tcctggcgac gccgggcaag cgacagcgcc gcatggcggt
      961 cgctccgtgg ggaacctggg gaagcggcgg actggtgctg cgggggaaat tctaaccatg
     1021 cgcgaaatcg cattttccgg caaaatggcc gtcatgttcc gccgctcgct tgccctcctg
     1081 accctgctcg cgggatgcac atccccggcg tatgaactct atctcgagca cgccgacgac
     1141 gcgggctcta cggtcgcctc gagcgacggc gctgcggaga cgacgggcga tgcgacgccg
     1201 acgagcacag gcgggaccgg cagcgacagc gcggacgcga cgggcgacac atccgacgcg
     1261 accgacacgg ccccgctgtc cgacagcgcg accgacagcg cgaccgacac gggcgacgcg
     1321 accgacaccg aggacagccc gccggtcggc gaggcggaca agccggtgat cgtctcgatc
     1381 gagctgccgg cgaacgtcta cacggccggc ccggtgccgc tcgccgtgca gaccgagggc
     1441 accgcggcgg tgcatgtaac gctcaatggc gctgacgcgg gcgagttgat cgccgcgggc
     1501 ggcgggctgt tcactggcgc gctgccggtc catggcgcga tcgacaacgg ctcgcatgcg
     1561 gtcgaggtca tcgccacgca gggcaagtac gaggaccgca agccggccat gttcaacgtc
     1621 tcaacacccg agccgggcac cgaggcgtgg tcgctgcccg gccccgatgg cagccgcacg
     1681 aatcgcgtcg cgctcacgcc cgcgggtgac ttgatcgagg tcggccagac cgtgatcggc
     1741 ggcgtaccgc gtccgacgat ccgcaagcgc tcgagcgtca ccggggcgga gctgtggccc
     1801 gagggcacga tcacgctcga cacgcgcgag ggcgccgtgg ttgacgtcgc ggccctgccg
     1861 gacgggcgca tgtgggtggc gatgaacgtg cggccgccgg gcaaggatca acggccgcgg
     1921 atcgtcctgc tggacgcgga cggccatgcg accggggtcg ggcttgaggg caccccgggc
     1981 cgcgtcgtgc gggccatcgc cgccgacgcg gacggcggct gcttcgcggt cggcctcgcc
     2041 gtggtcatgg gcgattggga catcgcctac tggcggatcg acgccgcaaa cgagccgacg
     2101 ctcggccacg cgttcgacta ctggccgccg aagcatccag agcacagctt catggatctc
     2161 gccagcgacg tcgtgatcga cggcgacgtc gcgtgggtcg tcggcatgag caaggggaag
     2221 cacgacgccg aacagaaaga cccggatttg tacctgcgcg gcgtcctcgt gccgctgaac
     2281 ctccacaccg gaaagctcgc cggttccgtc ctcgtcgccc cggcggcgcc tggctggtct
     2341 caaagcgcgc tcttcggcgc agcgctgcat cccgcgggcg tgctagcgac cggctacggg
     2401 tgcgacaaga cctgctccac gtatcggatc gagacctccc tctacagcgt cgacggcgtg
     2461 cgcacgtggt tcgagcacga ggccaagaac gacggcctcg cctacggcag cgacgtcgtg
     2521 ctcgatggcc aggggcgggc gctcgtctcg gccgcggtga cgcagaacgg caagttgcgc
     2581 ggctacgtgt tcggccgcaa ggtcggcctg cccggcccgg tcctgctcga gcactggtac
     2641 ccgggcgccg gcccctcgga ggccctcggc atcgcccgcg acgtcttcga tcgaatcttc
     2701 ccggtcggct acatcacctt caacggcgag acccaggccc gcgtcacgcg gatccacggg
     2761 taggctcgcg cggcgcgagg ccgtgcagcg aggtcactgc gggatgaagc tcccgcacgc
     2821 ctcgctgatc ttgtccgcgg cttgctcgaa gaacggcgcg aagctcggcg tacaggtgtc
     2881 gccgaatacg gcgtagggga accgcgacag cagatcctcg aagatgccga acgaaggctc
     2941 gtcccagtag tggcagtctt ccgcgggctc aaactccccc ggcgcgttcg ggacgaccgc
     3001 gagcatgacg acggcgctcg ggtccccctt ggccgcgatg atcgcctcgt actgctgctt
     3061 ggggtacgac ttcccctgat cgtgaaagtc cgtgatcagt gcgacgacga gcagcgcgtc
     3121 cttgcgcaaa aagccctcgt tgcacccgcc cggcccgttc agttcaggcg agagcgcggc
     3181 gatcatcgcg tcgcccatca gggccgcttt gccgcccact ccaaccttgg cgatgcactc
     3241 gaaggcgccc ggcatgtcgg gctcgccgct gacgatgtag cggcggccct cgtgcagctt
     3301 gcacagcttg ttcgccgcgt cgccgcccgc gttgaagatc agcccggcgc cgagctgctc
     3361 gtcgcacggc gtcatcatgt ccgggaacgt ctggcagttg tagtccgggg cgttcgggcc
     3421 gcaggtcggc cagttctcca cgttctcgca gccgtcgggc tggttctggc acgtccagcc
     3481 cggccagtag gcgtccgggt tagccgtcat gatgtggacg tcgaagccct cgagcgtctg
     3541 ctcgatggtc tcgatgaagc cggggaagct ggcgaggagc tgctcctgct cggtcaccat
     3601 cgtgccctgg cgcgagatca cgaacagcag gtcgaccttg cccttgcagc cgggcggctg
     3661 cgcgggcccg aagtccatca cgg
//
LOCUS       DASWNJ010002002         3270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_418959, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002002 DASWNJ010000000
VERSION     DASWNJ010002002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418959"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VGB85_33385"
     CDS             <1..458
                     /locus_tag="VGB85_33385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139044.1"
                     /translation="TARAALAAHVARHGPGSLHAGNVGLGLALILAARGDQAGALAEA
                     RRGVDLRRAHYGPHHRDTASGQLLLAGMLLDFDDTPAALAVLDEVAAVPELPADLTLE
                     LRFLRARADHDLGRTAEALAEVRRIVGDAAGPDIPPLFRDELVAWLATH"
     gene            465..599
                     /locus_tag="VGB85_33390"
     CDS             465..599
                     /locus_tag="VGB85_33390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139045.1"
                     /translation="MARSDISTARSQYKLPVHCHDGPAPLGSARWLAEIDAGTGPGVY
                     "
     gene            607..1953
                     /locus_tag="VGB85_33395"
     CDS             607..1953
                     /locus_tag="VGB85_33395"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0139046.1"
                     /translation="MDDATLAALREALTATPDNDPLRVVVFRALVARGEPRQAADLLQ
                     GRELRLGDDDRRQLAQLWLGLDEPQRALDLVPGTDAASFMLRARALVALGRHEHALAA
                     YNEALGKNAALEDLDLKARITARVSTPAAQRPGTVVPLRVLATDDTSGDEVKRLLQPE
                     QQKVTFADVGGHDDIKRQIEKRIITPFLKPSLFERFKKRVGGGILMYGPPGCGKTLLA
                     RATAGQCDAQFFNVAISDVLDMYIGESERKLAAIFAKARSSTPAVLFFDELEGLAAKR
                     SYARESSSAKVVSQFLTEMDGFAQNNKGVLILGATNVPWAVDPAFRRPGRFDRVLFVP
                     PPDRSARAAILRILLTGRPIKGEIDHDWLAERTSGFSGADLENLVEEAADAAIEDSLA
                     ASDEVPITGEHLRHALKQVKATTLEWLTTARNHARYSNEGGQYDEVLQFLDRNAKR"
     gene            1962..2471
                     /locus_tag="VGB85_33400"
     CDS             1962..2471
                     /locus_tag="VGB85_33400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139047.1"
                     /translation="MARSSYVIDDEPVPDAGLTRYIVNPLYPLLAVMLAGVWLAWPWM
                     LLNARALGSPHWRRQAQLLAVGLAGTVALALVIVALVNADVITTRTHARLALLAVTTW
                     KMAVSYKVHSLQLGTFALYSHYHGDPTAQFGVRLLMLGMFLRGIIDGLIPSILWIIIV
                     SGAPWTGEL"
     gene            2468..>3270
                     /locus_tag="VGB85_33405"
     CDS             2468..>3270
                     /locus_tag="VGB85_33405"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0139048.1"
                     /translation="MTDPTIDRWLEQAERMQRLGDHAAAISSLKQALTHDPEQPAAHA
                     LLAISLLSTRRLHAAAREAAEALHLAPELPLALYAYATVAMAQQRWPAAEAAVTTLLA
                     LEPNDVANLRLAAEFHRREDPTRARKHLERARELDPDDPHTLVDLGQLALQQNRLDEA
                     ATLAEAALGHAPELSEAHVVAGHVALARGDVPRAREHARTALTNDPQDHEALGLLTAI
                     KARENPLLGLWWRYQVFIGEPGSPRALGLLLGMFIAVRLVAIFADAWGAP"
BASE COUNT          479 a         1388 c          974 g          429 t
ORIGIN      
        1 ccaccgcccg cgccgccctc gccgcgcacg tcgcccgcca cggtccaggc agcctccacg
       61 cgggcaacgt cggcctgggt ctcgccctca tcctcgccgc ccgcggcgac caggccggcg
      121 ccctcgccga ggcccggcgc ggcgtcgacc tccgccgcgc ccactacgga ccccaccacc
      181 gcgacaccgc cagcggccag ctcctgctcg cgggcatgct cctcgacttc gacgacaccc
      241 ccgccgcgct cgcggtcctc gacgaggtcg ccgcggtccc cgagctcccc gccgacctca
      301 cgctcgagct ccggttcctc cgcgcccgcg ccgaccacga cctcggccgc accgccgagg
      361 ccctcgccga ggtccgccgg atcgtcggcg acgctgccgg ccccgacatc ccgccgctgt
      421 tccgcgacga gctcgtcgcc tggctggcca cccactgacg cgtggtggcc cgcagcgaca
      481 tctcgaccgc ccgctcgcaa tacaagctcc cggtccactg ccatgacggt cctgccccgc
      541 tcggctccgc ccggtggctc gccgagattg acgccgggac cgggccgggg gtctactgag
      601 gctccgatgg atgacgcgac cctcgcggcg ctgcgagagg ccctcacggc caccccggac
      661 aacgaccccc tgcgcgtggt cgtgttccgc gcgctggtcg cccgcggcga gccgcgccag
      721 gccgcggacc tcctgcaggg ccgcgagctc cggctcggcg acgacgaccg ccgccagctc
      781 gcccagctgt ggctcggcct cgacgaaccg cagcgcgccc tcgacctcgt gcccggcacc
      841 gacgccgcca gcttcatgct gcgcgcccgc gccctcgtcg cgctcggccg ccacgagcac
      901 gccctcgccg cctacaacga ggcactcggt aaaaatgccg cgctcgagga cctcgacctc
      961 aaggcccgga tcaccgcccg cgtctcgacc cccgccgccc agcgccccgg caccgtcgta
     1021 cccctgcgcg tcctcgccac cgatgacacc agcggcgacg aggtcaagcg cctgctccag
     1081 cccgagcagc aaaaggtcac cttcgcggat gtcggcggcc acgacgacat caagcggcaa
     1141 attgaaaaac gcatcatcac ccccttctta aaaccgtcgc tgttcgagcg gttcaagaag
     1201 cgggtcggcg gcgggatcct gatgtatggc ccgccgggct gcggcaagac cctgctcgcc
     1261 cgcgcgaccg ccgggcagtg cgacgcccaa tttttcaacg ttgcgatctc cgacgtgctc
     1321 gacatgtaca tcggcgagag cgagcgcaag ctcgccgcga tcttcgccaa ggcccgctcg
     1381 tcgacccccg cggtgctgtt ctttgatgag ctcgagggcc tcgcggccaa gcgcagctac
     1441 gcgcgcgaga gctcgtccgc gaaagtggtc agtcagttcc tgaccgagat ggacggcttc
     1501 gcccagaaca acaagggcgt gctgatcctc ggcgcgacca acgtcccctg ggccgtcgac
     1561 cccgcgtttc gccgccccgg ccgcttcgac cgcgtgctgt tcgtcccccc gcccgaccgc
     1621 agcgcccgcg ctgccattct tcgcatttta ctgaccggcc gtccgattaa aggcgagatc
     1681 gaccacgact ggctcgccga gcgcacttca ggcttctcgg gcgccgacct cgagaacctc
     1741 gtcgaggagg ccgccgacgc cgcgatcgag gactcgctcg ccgccagcga cgaggtcccg
     1801 atcaccggcg agcacctgcg ccacgcgctc aagcaggtca aggcgaccac cctcgagtgg
     1861 ctgaccaccg cccgcaacca cgcccgctac agcaacgagg gcggccagta cgacgaggtg
     1921 ctgcaattcc tcgaccgcaa cgcgaagcgc taaagaccac catggcccgt tcaagctacg
     1981 tgatcgacga cgaacccgtg cccgacgccg ggctcactcg ctacatcgtc aacccgctct
     2041 acccgctgct cgccgtcatg ctcgccggcg tgtggctcgc gtggccgtgg atgctgctca
     2101 acgcgcgcgc gctcggcagc ccgcactggc gccgccaggc ccagctgctc gccgtcggcc
     2161 tcgccggcac cgtcgccctc gcgctggtga tcgtcgccct cgtgaacgcc gacgtcatca
     2221 cgacccgcac ccacgcccgc ctggcgctgc tcgcggtgac gacgtggaag atggcggtca
     2281 gctacaaggt gcacagcctg cagctcggca ccttcgcgct ctacagccac tatcacggcg
     2341 acccgaccgc gcagttcggc gtgcgactgc tgatgctcgg catgttcctg cgcggcatca
     2401 tcgacggcct gatccccagc atcctgtgga tcatcatcgt cagcggagca ccctggacag
     2461 gcgaactatg accgacccca cgatcgaccg atggctcgag caggccgagc gcatgcagcg
     2521 gctcggtgac cacgccgctg cgatctcgtc gctcaagcag gccctcaccc acgaccccga
     2581 gcagcccgcc gcccacgccc tgctcgcgat ctcgctgctc tcgacccgac ggctccacgc
     2641 cgccgcccgc gaggccgccg aggccctgca cctcgccccc gagctgcccc tggccctcta
     2701 cgcctacgcg acggtcgcca tggcccagca gcgctggccc gccgccgagg ccgccgtcac
     2761 caccctgctc gcgctcgagc ccaacgacgt cgccaacctc cgcctcgccg ccgaatttca
     2821 ccgccgcgag gacccgaccc gcgcccgcaa acacctcgag cgcgcccgcg agctcgaccc
     2881 cgacgacccg cacaccctcg tcgacctcgg ccagctcgcc ctgcaacaga accgcctcga
     2941 cgaggccgcc acgctcgccg aggccgccct cggccacgcc cccgagttga gtgaggccca
     3001 cgtcgtcgcc ggccacgtcg ccctcgcccg cggcgacgtc ccgcgcgccc gcgaacacgc
     3061 ccgcaccgcc ctgacgaacg acccccagga ccacgaggcg ctggggctcc tgaccgcgat
     3121 caaagcccgc gagaacccgc tgctcggcct gtggtggcgc taccaggtgt tcatcggcga
     3181 gcccggcagc ccgcgcgcgc tcggcctcct gctcggcatg ttcatcgccg tccgcctcgt
     3241 ggcgatcttc gccgacgcct ggggcgcccc
//
LOCUS       DASWNJ010002003         3165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_418998, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002003 DASWNJ010000000
VERSION     DASWNJ010002003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418998"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2617)
                     /locus_tag="VGB85_33410"
     CDS             complement(<1..2617)
                     /locus_tag="VGB85_33410"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEY0139049.1"
                     /translation="MNGPTAALHERYAAWIDGALAADRLQRAAQAVVERHEVLRTHIA
                     ERDGTPVQLIADHISLPWRTLDVDDDDAALAAASEEAGRVLDWERGPLWRLSLFRVRA
                     DRHLLVLTMHHILSDGDWTHALFFGELHAQYHGRALSGSPLQYRDHVERQRQRLTDER
                     RAQELAYWCDELRDAPPLLDLCTDLPRPATPTFSGATAVRRLAPGTLARGRYLAGRLE
                     VDLYALLLASMLALLHRYSGQEDVLIGVPLDGRDDPEVRAVLGYFGGPAVIRGRFSDD
                     PTGSDLVRRVHAAVAGARSRGNLSFLHLVEALRPPRDSGRTPLFQVLFDLRTASSPLV
                     GAELRFTPVDVPLAAIAYELIVTATVAGDDLDLIVEYDAGLFAAATIERLLGHWQVLL
                     DGLLADPRRRASALPLLGEPERRVVVETWNRTLADYPREHAVHALFAAQAERTPHAIA
                     VECEGQELTYEALDRRANRAARRLLALGVGPNTRVAVCVPRSLELPVALLAILKTGAA
                     FVPIEPRYPEARIAMVLTDAEVAAVLLHGDTADRLPEIGVPRLRIDASSLDAEDGDEA
                     APVPGLASELPAYVIFTSGSSGRPKGVVVTHRSLVNHNWDFIRRTTLGPGDRVLQFAA
                     LGFDTALEELFPTLLAGATLVLQPGATELSFAALVQRMTALRITVFDPPTAYFHEWVL
                     DLAAARSPLPPTLRQLVLGGEEALVERVALFRELAGPQVAVLNTYGPTEATIVATTYP
                     VGADEDVRARVRMPIGRPNANVRAYVLDAALQPAPIGVPGELYLGGEGVALGYLGRPD
                     LTAERFIADPFVADPAARLYRTGDRARWLADGNLEFLGRVDHQVKLRGFRVELHEVEA
                     ALREQPGVRDGVALVD"
BASE COUNT          496 a         1133 c         1116 g          420 t
ORIGIN      
        1 cgtcgacgag cgcgacgccg tcgcgtacgc ccggctgctc gcgcagcgcg gcctcgacct
       61 cgtgcaactc gacccgaaag ccgcgcagct tgacctggtg atcgacgcgg ccgaggaact
      121 cgaggttgcc gtcggcgagc cagcgcgcgc ggtcgccggt gcgatacagc cgcgccgccg
      181 ggtcggcgac gaacgggtcg gcgatgaagc gctccgccgt caggtccggc cgaccgaggt
      241 atccgagcgc cacgccctcg ccgccgaggt acagctcgcc cggcacgccg atcggcgccg
      301 gctgcagcgc cgcgtcgagc acgtaggccc gcacgtttgc gttgggccgc ccgatcggca
      361 tgcgcacgcg cgcccgcacg tcctcgtcgg cgccgaccgg ataggtcgtc gcgacgatcg
      421 tcgcctcggt cggcccgtag gtgttgagca ccgcgacctg cggaccggcg agctcgcgga
      481 acagcgcgac gcgctcgacc agcgcctcct cgccgccgag caccagctga cgcagcgtcg
      541 gcggcagggg tgagcgcgcg gccgcgaggt cgagcaccca ctcgtggaaa tacgccgttg
      601 gcgggtcgaa caccgtgatc cgcagcgcgg tcatccgctg gaccagcgcc gcgaacgaca
      661 gctcggtcgc cccgggctgc agcaccagcg tcgccccggc gagcagcgtc gggaagagct
      721 cctcgagcgc cgtgtcgaag ccgagcgccg cgaactgcag cacgcggtcg ccgggcccca
      781 gcgtggtccg gcggatgaag tcccagttgt ggttcaccag cgagcggtgg gtgaccacga
      841 cccccttggg ccgcccgctc gagccggagg tgaagatcac gtaggctggg agctccgacg
      901 ccagaccggg caccggcgcc gcctcgtcgc cgtcctccgc gtcgagcgag ctcgcgtcga
      961 tgcgcaggcg cgggacgccg atctccggca accggtccgc ggtatcgcca tgcaaaagga
     1021 ccgccgcgac ctcggcgtcc gtcaggacca tggcgatgcg cgcctcggga tacctcggct
     1081 cgatcggcac gaacgccgcc ccggtcttga ggatcgccag cagcgcgacc ggcagctcga
     1141 gcgagcgcgg cacgcacacc gcgacccggg tgttcggccc gaccccgagc gccagcagcc
     1201 gccgcgccgc gcggttggcc cggcggtcga gcgcctcgta cgtcagctcc tggccctcgc
     1261 actcgaccgc gatcgcgtgc ggcgtgcgct ccgcctgcgc cgcgaacaac gcgtgcaccg
     1321 cgtgctcgcg cggatagtcg gccaaggtgc ggttccacgt ctcgacgacc acgcggcgct
     1381 ccggctcgcc gagcagcggc agcgccgagg cgcgccggcg cgggtcggcg agcagcccgt
     1441 cgagcagcac ctgccagtgc ccgagcaggc gctcgatcgt cgccgcggcg aacaatccgg
     1501 cgtcgtactc gacgatcagg tcgaggtcgt cgcccgcgac cgtcgcggtg acgatcagct
     1561 cataggcgat cgccgccagc ggcacgtcga ccggcgtgaa tcgcagctcc gcgccgacca
     1621 gcggcgacga ggcggtccgc aggtcgaaca gcacctgaaa cagcggcgtc cgcccggagt
     1681 cgcgcggagg tcggagcgcc tcgacgagat gaaggaagga gaggttcccg cgcgaccggg
     1741 ccccggcgac cgccgcgtgg acccgccgca cgaggtcgga gcccgtcggg tcgtcggaga
     1801 agcggccgcg gatcaccgcc ggtccgccga aatagccgag gaccgcgcgc acctcggggt
     1861 cgtcccgccc gtcgagcggg acgccgatca ggacatcctc ctggccgctg tatcggtgga
     1921 ggagcgccag catgctggcg agcagcagcg cataaagatc gacctccagg cgcccggcga
     1981 ggtagcgacc gcgggcgagg gtgccggggg cgaggcggcg gaccgcggtc gcgccggaga
     2041 aggtcggcgt cgccggacgc ggcaggtccg tgcagaggtc gagtaacggc ggggcgtcgc
     2101 gcagctcgtc gcaccagtac gccagctcct gcgcccgccg ctcgtcggtc agccgctgac
     2161 gctggcgctc gacgtgatcg cgatactgca gcggcgaccc ggacaatgca cgcccgtggt
     2221 attgtgcgtg caattcgccg aaaaacagcg cgtgcgtcca atctccgtcg gacaggatgt
     2281 ggtgcatggt gagcaccagg aggtgcctgt ccgcgcggac gcggaacaag ctgaggcgcc
     2341 acagcgggcc gcgctcccag tcgagcaccc ggccggcctc ctcgctggcg gcagcgaggg
     2401 ccgcgtcgtc gtcgtcgacg tcgagcgtcc gccacggcag tgaaatgtgg tcggcgatca
     2461 gctgcacggg cgtaccgtcg cgctcggcga tgtgggtccg caggacctca tgccgctcga
     2521 ccaccgcctg cgcggcccgc tgcagccggt ccgcggccag cgctccgtcg atccaggccg
     2581 cgtagcgctc gtgcagcgcc gccgtcggac cattcatttg aacgagcaac cacatccgct
     2641 gttgctcgaa agaaagcggc gcagagcgta atcgagacat cgcgcgtcct tcgggccgaa
     2701 gaagaggccc gggaaatcgg gtcaccactc taccatctta tgcatgctct cccaggcaat
     2761 taactgcata tcaatttccg cgcacttttc cgagatgggc tgaaaaccaa tgaccctgcc
     2821 gtctggcgac cacagcggtg aaaaataccc attcgtcgtg ccgcctaatc tgcgagcttc
     2881 gcgtgatcgt cgcccgacgc gcacgcgacg cggtctcacg ggggacgcac tggaacatgt
     2941 tcgaggggac ctgggccggt gggatatcgc ggtccatcga cgcacgggcg aggtgcccct
     3001 ggttttgctt gtctctggct cacggcgggc cctatactcc ggccctgtcg ttgagacgtc
     3061 cggtggtggt gggcggtttc aaccgcgatg aatcgtcgag aggagtttcg atgcatgaca
     3121 ttccgcatac gagccccgcc gaccccgacg aggcattcaa acgcc
//
LOCUS       DASWNJ010002004         2928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_347464, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002004 DASWNJ010000000
VERSION     DASWNJ010002004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347464"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1779)
                     /locus_tag="VGB85_33415"
     CDS             complement(127..1779)
                     /locus_tag="VGB85_33415"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0139050.1"
                     /translation="MSESKNFLETIFASTTAAADRPLTIEVHGKEQRPTTAAQLLELV
                     ARARTSLRSAGVKPGDRVALLGPNSSRWVAADLAILAEGAIVVPLYSRQDPRELAVMV
                     RDCSPALVLASDAQLGDALAAAWPEHGSIALYDEVFASEPTTADPHAIAPEDPVTIIY
                     TSGTSGDPKGVVLTRANVDYMVPQTATAVHEITGTRGAPDRVFHFLPLCFAGSRVMLW
                     TQLYRGNPILLSTDLQNLAQEMGTADAHYFLNVPALLERIRTGVGAKLRERGGIAASL
                     YQRGQDAFKKQRAGTANALDRVTLAIARKLVFGAIKKQVGPSLEFLICGSAALAEETQ
                     WWFELIGVRVYQVYGLTETTAIVTMDKPGQIEPGHVGHALRGCELKLDPDGELLVRGP
                     NIFSGYWNRPEATADAIRDGWFHTGDRCDLTATGNLKILGRVKNILVPESGHNIAPEP
                     IEQKFLDHCPAAQQCMLVGHARPYLTLIVPHAAAEIADADIQAAIDRVNADVPHYRKI
                     KKFLRVPEAFTIENGLLTANQKLRRRVVEDHHRAAIDRVYAS"
     gene            complement(1776..>2928)
                     /locus_tag="VGB85_33420"
     CDS             complement(1776..>2928)
                     /locus_tag="VGB85_33420"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEY0139051.1"
                     /translation="TVAQIEMPQLFPPFLYRECPKYHGVIACEGSMFKSKFASALSTM
                     MAGAMGMAIAERKLSVGYGAEAGAMTPSLKQFVADVCKGSLVLCRNEPSRAVLGELGI
                     RTTSGADTAWTFEPAPLPRGAQLLRDAGWDGRKKVLVVCPINPFWWPVKPDLLKATAH
                     KLGGQYKHEHYRSIYFHHHSDKATKQYETYLQALADGVNAFTKEQDVFTILVGMEQLD
                     RHACEDLAPRLDSPAPLFISDTYDMYDLVSVLRNCNMMVSSRFHAVVTSMPGLVPSIG
                     VTMDERIRNLMTDRGHPDMFFEVDEKDLGEKVLVSLRRLDRDAEAISAEIGKAVPKQL
                     ALMGQMGIDFMDEVVRVYPEFPRKDLPRTWEAHLPPLPPAVQRVLERHG"
BASE COUNT          435 a          895 c         1103 g          495 t
ORIGIN      
        1 tgtgggcgtc ggggtcgatc gaggcgagga aggcctcgag ctcggtcaac atggcggtgc
       61 cgatctcgtt gcacggcggg gcgccgagcg cgagctcgta gacgccgtcg tgataggaca
      121 tgacgatcat gacgcgtaca cccgatcgat cgcggcgcgg tggtggtcct cgaccacgcg
      181 acggcgcagc ttctggttgg cggtcagcag gccgttttcg atggtgaagg cctcgggcac
      241 gcgcaagaac ttcttgatct tgcggtagtg cgggacgtcg gcgttgacgc ggtcgatcgc
      301 ggcctggatg tcggcgtcgg cgatctcggc cgccgcatgg gggacgatca aggtcaggta
      361 agggcgcgcg tggccgacga gcatgcactg ctgggcagca gggcagtggt cgaggaactt
      421 ctgctcgatc ggctccgggg cgatgttgtg gccggattcg ggcacgagga tgttcttcac
      481 gcggccgaga attttcaggt tgccggtcgc ggtcaggtcg cagcgatcgc cggtgtggaa
      541 ccagccgtcg cggatcgcgt cggcggtcgc ctcggggcgg ttccagtagc cgctgaagat
      601 gttggggccg cggaccagca gctcgccgtc cgggtcgagc ttgagctcgc agccgcggag
      661 cgcgtggccg acatggccgg gctcgatctg gccgggcttg tccatcgtga cgatcgcggt
      721 cgtctcggtc aggccgtaca cctgatacac gcgcacgccg atcagctcga accaccactg
      781 ggtctcttct gcgagggcag ccgagccgca gatcaggaac tcgaggctcg ggccgacctg
      841 cttcttgatc gcgccgaaca ccagcttgcg ggcgatcgcg agggtgacgc gatcgagggc
      901 gttcgccgtg cctgcgcgct gctttttgaa ggcgtcctgg ccgcgctggt acagagatgc
      961 cgcgatgccg ccgcgctcgc ggagcttggc gccgacgccg gtgcggatcc gctcgagcag
     1021 ggcgggcaca tttaaaaagt agtgggcgtc ggcggtgccc atctcctgcg cgaggttctg
     1081 caggtcggtc gacagcagga tcgggttgcc gcggtacagc tgcgtccaca gcatcacgcg
     1141 cgagccggcg aagcacagcg ggaggaagtg gaatacgcgg tcgggcgcgc ccctcgtgcc
     1201 ggtgatctcg tgcacggcgg tggccgtctg cgggaccatg tagtcgacgt tggcgcgggt
     1261 cagcacgacg cccttgggat cgccgctggt gcccgaggtg tagatgatcg tgaccggatc
     1321 ctcgggggcg atcgcgtggg ggtcggcggt ggtcggctcc gacgcgaaga cctcgtcgta
     1381 cagggcgatg cttccgtgct cgggccacgc ggccgcgagc gcgtcgccga gctgcgcgtc
     1441 gctggcgagc accagcgcgg gcgagcaatc gcggaccatc accgcgagct cgcgcgggtc
     1501 ctggcggctg tacagcggga cgacgatcgc accctcggcg aggatcgcca ggtcggcggc
     1561 gacccagcgg ctcgagttgg gcccgagcag ggcgacacgg tcgccgggct tgacgccggc
     1621 gctgcgcaac gaagtgcggg cgcgggctac aagctcgagc agctgcgcgg cggtggtcgg
     1681 gcgctgctcc ttgccgtgga cctcgatcgt cagcggcctg tcggccgcgg cggtcgtgga
     1741 ggcgaagatc gtctcgagga aattcttgga ctcgctcatc cgtgacgctc caggacgcgc
     1801 tggacagcag ggggcagggg cggcaagtgg gcctcccagg tgcgtgggag gtccttgcgc
     1861 gggaactcgg ggtagacgcg gacgacctcg tccatgaagt cgatgcccat ctggcccatc
     1921 agcgcgagct gcttggggac ggccttgccg atctcggcgg agatggcctc ggcgtcgcgg
     1981 tcgaggcggc gcaggctgac gaggaccttc tcgccgaggt ccttctcgtc gacctcgaag
     2041 aacatgtccg ggtggccgcg gtcggtcatc aggttgcgga tgcgctcgtc catcgtgacg
     2101 ccgatcgagg gcacgaggcc gggcatcgag gtgacgaccg cgtggaagcg cgagctgacc
     2161 atcatgttgc agttacgcag cacgctgacg aggtcgtaca tgtcgtaagt gtcgctgatg
     2221 aacagcggcg cgggcgagtc gaggcgcggc gcgaggtcct cgcaggcgtg gcggtcgagc
     2281 tgctccatgc cgacgaggat cgtgaacacg tcctgctctt tggtgaaggc gttgacgccg
     2341 tcggcgaggg cctgcaggta ggtctcgtac tgcttggtcg ccttgtcgct gtgatgatga
     2401 aaatagatcg accggtagtg ctcgtgcttg tactgaccgc cgagcttgtg ggcggtcgcc
     2461 ttgagcaggt cgggcttgac gggccaccag aacgggttga tcgggcagac gacgagcacc
     2521 ttcttgcggc cgtcccagcc ggcgtcacgc agcagctggg cgccgcgcgg cagcggggcg
     2581 ggctcgaagg tccaggccgt gtctgcgccc gaggtggtgc ggatgccgag ctcgccgagc
     2641 accgcgcgcg agggctcgtt gcggcacagc acgagcgagc ccttgcacac gtcggcgacg
     2701 aattgtttca ggctcggggt catcgcgccg gcctcggcgc cgtaaccgac ggacagcttg
     2761 cgctccgcga tcgccatgcc catcgcgccg gccatcatcg tcgacagcgc gcttgcgaac
     2821 ttcgacttga acatcgagcc ctcgcaggcg atcacgccgt ggtacttcgg gcactccctg
     2881 tacaggaacg gcgggaacag ctgcggcatt tcaatctgcg cgaccgtt
//
LOCUS       DASWNJ010002005         1574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_347498, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002005 DASWNJ010000000
VERSION     DASWNJ010002005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347498"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..>1574)
                     /gene="tssI"
                     /locus_tag="VGB85_33425"
     CDS             complement(98..>1574)
                     /gene="tssI"
                     /locus_tag="VGB85_33425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HEY0139052.1"
                     /translation="YEHDDRRLLGDDGATRARRKQEQRAAQTRIYLGTSDVAELVPGV
                     FFSLNGHPHGALDRKYLIVAVRHAGEAPEEVLYASPTEDQVRYTNECECIEAERPWRP
                     APLFPKPRAHGLQTAIVVGPAGEEIHTDEYGRIKVRFHWDRRSPFGDPASCWIRVSQR
                     WAGPGWGSVFIPRIGMEVLVEFIDGDPDRPMVTGCVYNANNMPPYPLPEQKTKSTTKS
                     DSSPGGGGFNEIRLEDAKAAEEIFIHAQLDMNTVVLQHTTRNVGVDDTDTVGNNQVES
                     VGVDRTASVGNNEDLTVGVSQNRTIGGNQMTAIAANQLYSVGGAQDLIVGGTRTVSVS
                     GHQLTVVAMDRATSVGGNQGAQVFGASTVEVGLDRTTTILGSEIVTINMDHTATVLGA
                     CTVSIGTTVTASVGADVSAAIGANLSVSIGAAAQLVVAADRMIQIGGNDTLVVGGSLT
                     ISSPTITLSSGGATITLSGSNIDITASGIVKINGSLVETNT"
BASE COUNT          216 a          498 c          586 g          274 t
ORIGIN      
        1 ggccaccaaa catccgtaca tgcgctgctc gtcgaccacc gcccggatca ggcgcgccgg
       61 gaacggtgag gaattctcga gcttcatgac gggcgcgtca ggtgttggtc tcgacgagcg
      121 agccgttgat cttcacgatg ccggaggccg tgatgtcgat gttgctgccc gagagggtga
      181 tggtggcccc gccggacgac agcgtgatcg tgggtgaaga gatcgtgagg ctgccgccga
      241 ccacgagggt gtcgttgccg ccgatctgga tcatgcgatc ggccgcgacg acgagctggg
      301 cggcggcgcc gatgctgacg ctgaggttgg cgccgatggc ggcgctgaca tcggcgccga
      361 cgctcgcggt gacggtggtg ccgatgctga ccgtgcacgc gccgagcacg gtggcggtat
      421 ggtccatgtt gatggtgacg atctcggagc cgaggatcgt cgtggtgcgg tcgaggccga
      481 cctcgacggt cgaggcgccg aacacctgcg cgccctgatt gccgccgacc gaggtcgcgc
      541 ggtccatggc gaccaccgtc aactggtggc cgctgacgct gacggtccgg gtaccgccga
      601 cgatcaggtc ctgggcgccg ccgaccgagt acagctggtt ggccgcgatc gccgtcatct
      661 ggttgccgcc gatcgtgcgg ttctgactga caccgacggt caggtcctcg ttgttgccga
      721 ccgacgcggt gcggtcgacg ccgaccgact cgacctggtt gttgccgacg gtgtcggtgt
      781 cgtcgacgcc gacgttgcgc gtcgtgtgct gcaggacgac cgtgttcatg tcgagctggg
      841 cgtggatgaa gatctcttcg gcagccttcg cgtcctcgag gcggatctcg ttgaagccgc
      901 cgccgccggg gctcgagtcc gacttggtcg tgctcttggt cttttgctcg gggagcgggt
      961 agggcggcat gttgttggcg ttgtagacgc agccggtgac catcggccgg tcggggtcgc
     1021 cgtcgatgaa ctcgacgagc acctccatgc cgatccgcgg gatgaacacc gagccccacc
     1081 cggggccggc ccagcgctgc gacacacgga tccagcacga ggcggggtca ccgaagggcg
     1141 agcggcggtc ccagtggaag cggaccttga tccggccgta ctcgtcggtg tggatctcct
     1201 cgcctgcggg gccgacgacg atcgcggtct gcaggccgtg cgcgcgcggc ttggggaaca
     1261 gcggcgccgg acgccacggg cgctcggcct cgatgcactc gcactcgttg gtgtaacgga
     1321 cctgatcctc ggtcggcgag gcgtagagga cctcctccgg ggcctcgccg gcgtggcgca
     1381 ccgcgacgat caggtacttg cggtcgagcg ccccgtgcgg gtgaccgttg aggctgaaga
     1441 acacgccggg cacgagctcg gcgacgtcgc tggtcccgag gtatatgcgg gtctgcgcgg
     1501 cccgctgctc ctgcttgcga cgcgcccgcg tcgcaccatc gtcgccgagc aggcggcggt
     1561 cgtcgtgttc gtag
//
LOCUS       DASWNJ010002006         2662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_176347, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002006 DASWNJ010000000
VERSION     DASWNJ010002006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176347"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1244)
                     /locus_tag="VGB85_33430"
     CDS             complement(24..1244)
                     /locus_tag="VGB85_33430"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEY0139053.1"
                     /translation="MRTRMQAALAMHLALATCSPGQGTGTSSGDTSETGKTSEVTPTT
                     STTAQPGNLPPVAEIVGDLGEDLAAPLRVELASGSSDPDGSIVAVHWDFGDGASADGE
                     KVSHVYATEGTYELRLTVVDDDGASGETTRAVSVGGCPRFQPGKSVGKLATEALTEAS
                     GLAFSRQTPGVVWTHNDSEDAPRLYTFTDLGAPLGTYALQGVEVRDWEDMALGPGPKQ
                     GQDYLYVGDIGDNNAEYAAVFVHRVAEPPVDPATTGVDASLGGVESFQLTLPGGPRNC
                     ETMLVDPVSGDLYLVSKSVDGVSLVFRAAAPLKSGMLTEVATLELGAGGLTTGGSVTA
                     AGDWVVVRTYFAVRMWHRAAGTALHKAFAGAGCEVPVAMEMQGEAIGFAAQGLDYYTT
                     SEGAQPPLYRFTRG"
     gene            complement(1250..2176)
                     /locus_tag="VGB85_33435"
     CDS             complement(1250..2176)
                     /locus_tag="VGB85_33435"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0139054.1"
                     /translation="MGTQEGLSQAAEEQTLAAGTVLSVYYRVVKLVGPASFGTLYVGE
                     HIQMKSRVAIRVLSAQWKNWIRGEEDIERFRRAVRDASQTCHPNIAQVFDAGRLPDGR
                     LYMITELLVGHNLYEEIQQHQYLPVVRACRILRDVGRAVRAAHEVGIIHRDLKADNVM
                     LVPLPGTDGEAVQVLDFGIAAWAEVEARKTSPGVFVATPEYMAPEQVQGKPATQLFDI
                     YALGVMLFEAVVGEPPFAGNNSEFILKSKVARPAPSLAQKLPGMPRRLIELVDDCLLM
                     DPALRPQSAREFLVRIDEILRTLQRLSGPTPI"
     gene            2409..>2662
                     /locus_tag="VGB85_33440"
     CDS             2409..>2662
                     /locus_tag="VGB85_33440"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanoglobin"
                     /protein_id="HEY0139055.1"
                     /translation="MAPEEEVYARIGEQGFARLAAAFYRRIPGDDLLGPMYPRDDLAG
                     AERRLCDFLVGRFGGPQRYIALRGHPRLRMRHMPFVVDVAA"
BASE COUNT          364 a          948 c          935 g          415 t
ORIGIN      
        1 tcgcgatcgt gtacctgtga tgctcagccg cgcgtgaagc ggtacagcgg cggctgggcg
       61 ccctcggagg tcgtgtaata gtcgaggccc tgcgccgcga agccgatcgc ctcgccctgc
      121 atctccatcg ccaccggcac ctcgcagccg gccccggcga acgccttatg caaggccgtg
      181 ccggcggcgc ggtgccacat gcggaccgcg aagtaggtgc gcacgaccac ccagtcgcca
      241 gcggccgtca ccgacccgcc cgtggtcaac ccgcccgcgc cgagctcgag cgtcgccacc
      301 tcggtgagca tccccgactt gagcggcgcc gcggcgcgga acaccagcga gacgccgtcg
      361 accgacttgc tgacgaggta gagatcgccg ctcaccgggt cgaccagcat cgtctcgcag
      421 ttgcgcggcc cgcccggtaa ggtgagctgg aacgactcga cgccgccgag gctggcgtcg
      481 acgcccgtgg tcgcggggtc gaccggcggc tcggcgacgc ggtggacgaa gaccgcggcg
      541 tactcggcgt tgttgtcgcc gatgtcgccg acgtacaggt aatcctgacc ctgcttggga
      601 cctggcccga gggccatgtc ctcccagtcg cgcacctcga cgccctgcag ggcgtaggtg
      661 cccagcggcg cgccgaggtc ggtaaaggtg tagaggcgcg gcgcgtcctc ggagtcgttg
      721 tgcgtccaga ccacgccggg cgtctgccgg ctgaaggcca ggccggacgc ctcggtcagc
      781 gcctccgtgg cgagcttgcc gacggacttg ccgggctgaa accgcgggca accaccgacg
      841 gagaccgcgc gcgtcgtctc gccgctcgcc ccgtcgtcgt cgacgacggt caggcgcagc
      901 tcgtacgtgc cctcggtcgc gtacacgtgg gagacctttt cgccgtccgc ggacgcaccg
      961 tcgccgaagt cccagtggac cgcgacgata ctgccgtccg ggtcgctcga gccgctcgcc
     1021 agctcgacgc gcagcggcgc cgcgaggtcc tcgccgaggt ccccgacgat ctcggcgacg
     1081 ggcgggaggt tgccgggctg cgccgtcgtg ctggtcgtcg gcgtgacctc gctggtcttg
     1141 ccggtctcgc tcgtgtcgcc cgagctggtc ccggtgccct gtcccggaga acatgtcgcg
     1201 agcgccaggt gcatcgcgag cgccgcctgc atccgcgtgc gcatggcgtt tagatcggcg
     1261 tcgggccgga caggcgttgc agggtgcgca ggatctcgtc gatgcggacc aggaactcgc
     1321 gggccgactg cgggcgcagg gcggggtcca tcagcaggca gtcgtcgacc agctcgatca
     1381 ggcggcgcgg catgccgggc agcttctgcg ccagcgaggg ggccggacgg gcgaccttcg
     1441 acttgaggat gaactcgctg ttgttgccgg cgaacggcgg ctcgccgacc acagcctcga
     1501 agagcatcac gccgagcgcg tagatgtcga acagctgggt cgccggcttg ccctgcacct
     1561 gctcgggcgc catgtactcg ggcgtcgcca cgaacacgcc cggggaggtc ttgcgcgcct
     1621 cgacctcggc ccacgcggcg atgccgaagt cgagcacctg gaccgcctcg ccgtcggtgc
     1681 cgggcagcgg caccagcatg acgttgtcgg ccttgaggtc gcgatgaatg atgccgacct
     1741 cgtgggccgc ccgcaccgcg cggccgacgt cgcgcaagat cctgcacgcg cgcacgaccg
     1801 ggaggtactg gtgctgctgg atctcctcgt agaggttatg accgacgagc agctccgtga
     1861 tcatgtacag gcggccgtcg ggcaggcggc cggcgtcgaa cacctgggcg atgttcgggt
     1921 ggcaggtctg gctcgcgtcg cgcacggccc ggcgaaagcg ctcgatgtct tcctcgccgc
     1981 ggatccaatt cttccactgc gcactgagca cgcggatcgc cacgcggctc ttcatctgga
     2041 tgtgctcgcc gacgtacagc gtgccgaagc tcgccgggcc cacgagcttg acgacgcggt
     2101 agtagaccga cagaacggtc cccgccgcga gggtctgctc ctcggccgcc tgcgacagtc
     2161 cttcctgcgt gcccatcgtg tgctgcggtc cccgtacagg gaagagcgca aggctaccac
     2221 gccctgtcgc ggacgcggca caggcggcgt ggtttgccgt gatccggcgc tgtctccggc
     2281 ccgggccggg caaatcgcct gtgaaacatc gtttatttcc gcgccggagc ctcgctatgc
     2341 cccggcgcac gcgatcgcgg aggccccgcc agctgccttg cgcggcttcc gtggcatcat
     2401 tcgccgcgat ggcccccgag gaagaggtct atgcccgcat cggtgagcaa ggctttgcgc
     2461 gcctggccgc cgccttttat cgccggatcc ccggcgacga tctgctcggg ccgatgtatc
     2521 cccgggacga tctcgcgggg gccgagcggc gcctgtgcga cttcctggtc ggccgcttcg
     2581 gtggacccca gcgctacatc gcgctgcgcg ggcacccgcg gctacgcatg cgccacatgc
     2641 ccttcgtcgt cgatgtcgcg gc
//
LOCUS       DASWNJ010002007         5920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_347542, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002007 DASWNJ010000000
VERSION     DASWNJ010002007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5920
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347542"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..396)
                     /locus_tag="VGB85_33445"
     CDS             complement(97..396)
                     /locus_tag="VGB85_33445"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0139056.1"
                     /translation="MAPDRLRRFAALGDLHAEDERLAHVLTWLKGQQVEALLSVGDIV
                     DGAGDLDRCCALLAAHAAIVVRGNPPDLHQSRDPASWLSGELRNRRPRARAGRVS"
     gene            complement(402..962)
                     /locus_tag="VGB85_33450"
     CDS             complement(402..962)
                     /locus_tag="VGB85_33450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139057.1"
                     /translation="MRRLYVVFVANVCGAPVGCGGEGAEDTGETASGTTGETTGETTA
                     PTTGEATSGGGSGEYTAFYVPPGNGQYTRIVVRRVDVEENLCATVGFISLPEIEEGYA
                     ITTPAMWAAEWAFVQQEGTDCLSVYQLASEPADAESGLGTVTWPQDVACPPTVDIDVT
                     LKFASEPAWAPAEVQLKAEGIAVQGC"
     gene            1024..1314
                     /locus_tag="VGB85_33455"
     CDS             1024..1314
                     /locus_tag="VGB85_33455"
                     /inference="COORDINATES: protein motif:HMM:NF025715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HEY0139058.1"
                     /translation="MLEDVQKLRETLAALHSELGTVESGDPEVRRMLAGALQDIADKL
                     QSRDAALPADPPPEASVSAGQLAESARILEVEHPTLAATLRGVVEALTRMGI"
     gene            complement(1319..3181)
                     /locus_tag="VGB85_33460"
     CDS             complement(1319..3181)
                     /locus_tag="VGB85_33460"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEY0139059.1"
                     /translation="MSSEARSLRPAGWLWLAAMAVGPGLALWAVRQADPAAVVMRDEA
                     QGRGGAALWAGGSAALLAVVLAVWSRRAGVIEALSGLVARGRWLALLPLWPLLRFEPA
                     GVGGALAPMLVVVLGGVVAWSVYGFANMSQGTWRGGQALLASPRACALALAAAGAAVL
                     VRLSELALLRHHALISRVFDLGIYDNLMWNTMHGRLLACGFVRGGSHVTAHVDPLLIL
                     LTPIYALSPGPEVLLVVQAAAVLSGAAPVYLLALRRLGRPWLALVLGLGYLLHPSVHG
                     TILFDVHSLAFAAPLCLWALYFLECGATRRYALMIALLLLTREDVALLVCGLGLYAAL
                     GGGRRRLGLVTVAAAVVYFVTVKWFVQFTPYQYARRYAAFLPEEGGGFAAVAASVLSN
                     PAHAVYEALALRKLVFVLVLLAPLLLLPVLAGSLWWTFAFGLAFTLLASNTMNYFALS
                     HHTVMLFPVFFAAAPAGIDRAAEFFERFGGDRGRAVRALGVGVLVASLLATQRMGALI
                     KNGSFAGWPHAVPYALDEAMLARLQWVRAVEVGLPREASLAVSNSVGAHFSARDNVYF
                     YPEVKDADYLVLARLDVHPKGGAMNVRGLERRGYTLIDRHEDEIFVLRRTGQAP"
     gene            3246..4820
                     /locus_tag="VGB85_33465"
     CDS             3246..4820
                     /locus_tag="VGB85_33465"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY0139060.1"
                     /translation="MRLLALLALALSACHPAAADPPSRSQDNPAIDLVELDITEARRR
                     MNEGTLTSQDLTRAYLARIAELDDAGPMLGAVIELDPKALEYARLADRDRGGSKRGPL
                     HGIPVLLKDNIDVAGMVNSAGSLAMAGNRPAKDAFLVARLRAAGAVVLGKTNLSEWAN
                     FRSTKSISGWSSRGGQTRNPYALDHNPCGSSSGTGVAISASLAAVGVGTETDGSILCP
                     AAVSGLVGLKPTVGLISRAGIIPISATQDTPGPMARTVTDAAILLGAMVGQDPADPLT
                     TNAPTITDYTIGLRPDALTGKRIGVLRQAMGYDPIVDATTERAIAALRAAGAVIVDPA
                     EISTWEQWGAHELEILLFEFKAGLDAYLAHSGGPIHNIADLIAYNQAHAREVMPLFGQ
                     ELLLQAQAHGGLDSPAYLQARSEARRLAWDEGLRATLERDRLDALVAPTTGPAWPTDP
                     VNGDRIGGAGYGVAAVAGTPSLTVPMGDHHGLPLGLVFLGAPFSEAHLLAYAYAFEQA
                     THARRPPAYHPTLPAK"
     gene            4895..5698
                     /locus_tag="VGB85_33470"
     CDS             4895..5698
                     /locus_tag="VGB85_33470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139061.1"
                     /translation="MLSCRCAPLLLALVVPFACSSSENQVGATEATADTAPSTGAGDG
                     TAPATGSESTSADATTSDTGNPTSPTSPTSPTTPTTGETATTGEPAGSTSTGEGATCD
                     FPGVYDCCCFSVEGDPGKGILSISCPDPGILCDVPQATCPEGQTDCDLAALKITSAEG
                     LECALQALAAGTPGAISWGISSENGLNGGNHTLFVQEDGSVIVSGYEYKELDYTYTAV
                     ERRAKQDAAFFTGCVAKPDPERFECLRQAATGEPTETCLDGFSGNAGQD"
BASE COUNT          976 a         2433 c         1765 g          746 t
ORIGIN      
        1 cgccggcacc acgcgccggt gagcattttt ctctaaacgg gcaacgcgac gacctcggcc
       61 tgctcgatca cgatattgcc acccgtaccg gccacctcat gaaactcgac ctgcccgcgc
      121 tcgaggtcga cgattgcgaa gctcgccgga taaccacgat gcagggtccc gggattgatg
      181 aagatcaggg gggttgccgc gcaccacgat cgcagcgtgg gcggccagca gcgcgcagca
      241 gcggtcgagg tcgcccgcgc cgtcgacgat gtcaccgacc gacagcagcg cctcgacctg
      301 ctgtcccttg agccaggtca gcacgtgggc gaggcgctcg tcctcggcgt gcaggtcgcc
      361 gagcgccgcg aagcgtcgca gccggtcagg tgccatcgcg ctcaacatcc ctgcactgcg
      421 atcccctcgg ccttgagctg cacctcggct ggcgcccacg ccggctccga cgcgaacttc
      481 agcgtcacgt cgatgtcgac cgtcggtggg caggcgacgt cttgcggcca cgtcacggtg
      541 ccgagcccgc tctcggcgtc cgccggctcc gacgcgagct gatacaccga caggcagtcc
      601 gtcccctcct gctgcacgaa cgcccactcc gcggcccaca tcgccggcgt cgtgatcgca
      661 tacccttctt cgatctccgg cagcgagatg aaccccaccg tcgcgcacag attctcctcg
      721 acgtcgaccc tgcgcaccac gatccgggtg tactgcccgt tgccgggcgg cacataaaac
      781 gcagtgtact cgcccgaccc cccgccgctc gtcgcctcgc ccgtcgtcgg cgccgtcgtc
      841 tcacccgtcg tctcgcccgt cgtcccgctc gccgtctcgc cggtgtcctc ggccccctcg
      901 ccgccgcagc cgacgggcgc gccgcagaca ttcgcaacaa aaaccacgta caggcgccgc
      961 atgcctcctt gtcgccgacc ccaccgggcc cgacaagcgc ccgacccgtg gtacgctgac
     1021 cacatgctcg aagacgtcca gaagctccga gagacactgg ccgcgctgca cagcgagctc
     1081 ggcaccgtcg agtcggggga ccccgaggtc cgtcgcatgc tcgcgggtgc cctgcaggac
     1141 atcgccgaca agctccagtc ccgcgacgcg gctctgcctg cggacccgcc cccggaggcg
     1201 agcgtcagcg ccggtcagct cgccgagtcc gcgcggatcc tcgaggtcga gcacccgacc
     1261 ctcgccgcca ccctgcgcgg cgtcgtcgag gccctgaccc gcatgggcat ctgagccctc
     1321 agggcgcctg ccccgtgcgc cgcagcacga agatctcgtc ctcgtggcgg tcgatcagcg
     1381 tgtagccgcg gcgctcgagc ccacgcacgt tcattgcccc gcctttgggg tgcacgtcga
     1441 ggcgagccag caccagatag tccgcgtcct tgacctcggg ataaaagtac acattgtccc
     1501 gcgccgagaa gtgcgccccg accgaattgc tgaccgccag cgaggcctcg cgcggcagcc
     1561 cgacctcgac cgcgcgcacc cactgcagtc gcgcgagcat cgcctcgtcg agcgcatacg
     1621 gcaccgcatg cggccacccg gcgaagctgc catttttgat gagcgccccc atgcgttgcg
     1681 tcgccagcaa gctcgccacc agcaccccga ccccgagcgc ccgcaccgca cgcccgcgat
     1741 cccccccgaa tctttcaaaa aattccgcgg cccgatcgat ccccgccggc gccgccgcga
     1801 agaacaccgg gaacagcatc accgtgtggt gtgacagcgc gaagtagttc atcgtgttgc
     1861 tggcgagcag cgtgaacgcc agcccgaacg cgaaggtcca ccacagcgac cctgccagca
     1921 ccggcagcag cagcagcggc gccagcagca ccagcacgaa caccagcttg cgcagcgcca
     1981 gcgcctcgta caccgcgtgc gccgggttac tgagcaccga agccgccacc gccgcgaacc
     2041 ctccgccctc ctccggcaag aacgcggcgt atcgccgcgc atactgatac ggcgtgaatt
     2101 gcacgaacca cttcaccgtc acaaaataca ccaccgccgc cgcgaccgtc accagcccga
     2161 gcctgcgccg cccgcccccg agcgcggcat acaggccgag cccacacacc agcagcgcga
     2221 catcctcgcg cgtcagcaac aacagcgcga tcatcagcgc atatcgccgg gtcgccccac
     2281 actccaaaaa atacagcgcc cacaggcaca gcggcgccgc gaacgcgagc gagtgcacgt
     2341 cgaacaggat cgtgccgtgc accgacggat gcagcagata cccgagcccg agcaccagcg
     2401 ccagccacgg ccgcccgagc ctgcgcagcg ccagcaggta caccggggcc gcccccgaca
     2461 gcaccgcggc cgcctgcacc accagcaaca cctccggccc cgggctcaag gcatagatcg
     2521 gcgtcaacaa tatcaataat ggatcgacgt gcgccgtcac gtggctgccg ccgcgcacga
     2581 agccgcacgc cagcagccgc ccgtgcatcg tgttccacat caaattgtca tagatcccga
     2641 ggtcgaacac ccggctgatc agcgcgtggt ggcgcagcag cgccagctcc gacaggcgca
     2701 ccagcaccgc cgcccccgcg gccgccagcg ccagcgcgca ggctcgcggc gaggcgagca
     2761 gcgcctgtcc tccgcgccat gtcccttgcg acatgttcgc aaagccatac accgaccacg
     2821 cgaccacccc gccgagcacg accaccagca tcggcgccag cgccccgccg actcccgccg
     2881 gctcgaagcg cagcagcggc cacaggggca gcagcgccag ccagcgcccg cgcgcgacca
     2941 gcccggacag cgcctcaatc actcccgccc gccggctcca caccgcgagc acgaccgcca
     3001 gcagcgccgc gctgcccccg gcccacagcg cagctccacc gcgcccctgc gcctcgtcgc
     3061 gcatcaccac cgccgcgggg tctgcctgac gcaccgccca cagcgccagc cctggcccga
     3121 ccgccatcgc ggcgagccag agccatcctg cggggcgcag cgagcgggcc tcggacgaca
     3181 tcggcccctt gtataccgcg acccgcgccg ggcgtccccg gcggtccgcg ttacactccg
     3241 gccggatgcg cctgctcgcc ctgcttgccc tggccctgtc cgcctgtcac ccggccgccg
     3301 cggacccccc gtcgaggtcg caggacaacc ccgcgatcga cctcgtcgag ctcgacatca
     3361 ccgaggcccg gcggcgcatg aacgagggca ccctgacctc gcaagatctg acgcgcgcct
     3421 acctcgcgcg gatcgccgag ctcgatgatg ccggcccgat gctcggcgcc gtcatcgagc
     3481 tcgacccgaa ggcgctcgag tacgccaggc tcgccgaccg cgatcgcgga ggcagcaagc
     3541 gcggccccct gcacggcatc ccggtgctgc tcaaggacaa catcgacgtc gccggcatgg
     3601 tcaactccgc tggctcgctc gcgatggccg gcaaccggcc cgcgaaagac gccttcctcg
     3661 tcgcgcgcct gcgggccgcc ggcgcggtcg tcctcggcaa gaccaacctc agcgagtggg
     3721 ccaacttccg ctcgacgaag tcgatctccg gctggagcag ccgcggcggc cagacccgca
     3781 acccctacgc gctcgaccac aacccctgcg gctcgagctc cggcaccggc gtggcgatct
     3841 cggcgagcct cgccgccgtc ggcgtcggca ccgagaccga cggcagcatc ctgtgcccgg
     3901 ccgccgtctc cggcctggtc ggcctcaagc caaccgtcgg cctgatctcc cgcgccggca
     3961 tcatcccgat ctccgccacc caggacacgc cgggcccgat ggcgcgcacc gtcaccgacg
     4021 ccgcgatctt gctcggcgcg atggtcggcc aggaccccgc agatccgctc accaccaacg
     4081 caccgacgat cacggactat acgatcggcc tgcgccccga cgcgttgacc ggcaagcgca
     4141 tcggcgtatt gcgtcaggcg atgggctacg acccgatcgt cgacgcgacc accgagcgcg
     4201 cgatcgcggc cctgcgcgcc gccggggccg tcatcgtcga ccctgccgag atctcgacgt
     4261 gggagcaatg gggcgcgcac gagctcgaga tcctgctctt cgaattcaag gccggcctcg
     4321 acgcctacct cgcacattcg ggcggcccca ttcacaacat cgccgacctg atcgcctaca
     4381 accaggccca cgcgcgcgaa gtgatgccat tgttcggcca ggagctgctg ctgcaggccc
     4441 aggcccacgg cggcctcgac tcgcccgcat atttgcaagc ccgcagcgag gcccgccgcc
     4501 tcgcctggga cgagggtctg cgcgccaccc tcgagcgcga ccgcctcgac gccctcgtcg
     4561 ccccgaccac cggccccgcc tggccgaccg accccgtgaa cggcgaccgc atcggcggcg
     4621 ccggttatgg cgtcgccgcg gtcgccggca ccccgagcct caccgtgccg atgggcgacc
     4681 accacggcct cccgctcggc ctcgtatttc tcggcgcccc cttctccgag gcgcatctgc
     4741 tcgcatatgc atacgccttc gagcaggcca cgcacgcccg tcgcccgccg gcctatcacc
     4801 cgaccttgcc ggcgaagtga gccgacgccc gctcacccgt accagcggcg tcaccgccgg
     4861 gttgcagccg ggcgatcctc ggccacatag ccccatgctc tcttgccgct gcgcccccct
     4921 gctcctcgcg ctcgtcgtgc ccttcgcctg ttcgtccagc gagaaccagg tcggcgcgac
     4981 cgaggccacc gccgatactg ccccgtccac gggcgccggt gatggcaccg cccccgccac
     5041 cggcagcgag tcgaccagcg ccgacgcgac gaccagcgac accggcaacc cgaccagccc
     5101 gaccagcccg accagcccga ccaccccgac caccggcgag accgcgacca ccggcgagcc
     5161 ggccggcagc acgagcaccg gcgagggcgc gacctgcgac ttccccggcg tatacgactg
     5221 ctgctgcttc agcgtcgagg gcgaccccgg caagggcatc ctgagcatct cctgccccga
     5281 ccctggcatc ctgtgcgacg tcccgcaggc gacctgtccc gaaggccaga ccgactgcga
     5341 cctcgccgcg ctcaagatca ccagcgcgga gggcctcgag tgcgccttgc aggcgctcgc
     5401 ggccggcact ccgggcgcga tctcgtgggg gatcagctcc gagaacggcc tcaacggcgg
     5461 caaccatacc ctgttcgtcc aggaggacgg cagcgtgatc gtctccggct acgaatacaa
     5521 ggagctcgac tacacctaca ccgccgtcga gcggcgcgcg aagcaggacg cggcgttctt
     5581 caccggctgc gtcgccaagc ccgaccccga gcgcttcgag tgcctgcgcc aggccgccac
     5641 cggcgagccc accgagacct gcctcgacgg cttctccggc aacgccggcc aggactgagc
     5701 tccgcgccca cccggcccat tggcacccgc cccgcggctt gttaccgtgc ggcggggcgc
     5761 caatgcagat ccagctcgtc agccacgcct ccgtcatcgt ccgcaccacg gacacgcaga
     5821 tctggaccga cccctggctg accggcaaag tttttaataa ttcgtggacc ctgctggtcc
     5881 cacccgcgtg ggaccccgcg ctgctcgatg ccatcgatta
//
LOCUS       DASWNJ010002008         3993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_388186, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002008 DASWNJ010000000
VERSION     DASWNJ010002008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388186"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2100)
                     /locus_tag="VGB85_33475"
     CDS             complement(<1..2100)
                     /locus_tag="VGB85_33475"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY0139062.1"
                     /translation="MQRRPVAVSSSFTSTVLSASLWTVALLLVGCTSTKPADEATPDK
                     PADAATQPAGLDVWAGVPPITPLPDDITTELRPVPGPQPPPSVSERVELPFPPPAPPS
                     KQTQDPAATGPLQVLRTSPVMTEPGLVGSVTAVFNQPMIPLASIDELKLERSPMSITP
                     QPPGKFRWLGTQMIAFEAEGRVPFSTTYTATVEAGEASTIGNKLGKRVQWKFSTPVLA
                     LDSAMPSEWDTAELEPHIVLLFNQDIQRERLAAAVKLKGGNTSVGLELVPVSGYATLP
                     EPYKSQALAGRAERTLVLRPNGKLRPNTQYVVTVPAGAYGEGPNPSKALTSKFRTYPP
                     LTLSAPHCNEAYAWDCSPAGGVTLGASTVVVDDPELPGKVHVTPEVPDLEVTGGGGIY
                     LRGKFRGLGTYTVTVDAGVRDIHGQTLASPFRTTFTLRPLDPSLVVDGVTHDPIVLEP
                     SHAGVLDLKAAGLTQVEVRARSVSPAELRRVLSERNEYQGDDQWPLPLQQAKETVTDV
                     SGSRVEPVTVPLTVRDHAAMPGNLMLFGARTNKIGDGDWKYRQQIGGLVEVTRLGVSA
                     AIDSDSGVILVTDLVTGEPLAGVDLALHVQAQDTPAWTGRTDARGLAELTHGVIYDQP
                     YLLATTKDDVAYVPLQGSLDGSWSNWQGGGGDNEPRAFFFTDREPYKPGDTVHLSGIV
                     REEVRGPKGGVVPYRNDL"
     gene            2524..3492
                     /locus_tag="VGB85_33480"
     CDS             2524..3492
                     /locus_tag="VGB85_33480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139063.1"
                     /translation="MSRDDVLRTFAPLLHSFGHGPAPVATKAEAAEQARAREIVDRAA
                     SYTIESIVKSLADAQVELGGAIDGLADQLEREASKLDELRHALGSESRRLEDLRNTRV
                     AAEALEILIQDHKKKDETLDAKATSERLDLETKVATQHSAWAREEAERSQARAEAEAA
                     QARERKAKEEQYTYEIERQRKLSTDEFAEKKRLLERTLSETGAAKDKNWGERETLLAA
                     ASGEIEALRARAEAAPKQLEDETRKARERAIARITAEAKHEADLQERESAANIEVFEL
                     RIKALEERIGRQLNQLADLQRQLGTALEQGQNLAHKAIEGTSGGKK"
     gene            3496..>3993
                     /locus_tag="VGB85_33485"
     CDS             3496..>3993
                     /locus_tag="VGB85_33485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139064.1"
                     /translation="MATKTFTSRSTKTEILKAYNELAKELKQGRGAAPAESRASTSSA
                     SSASSTADADEPTVNTASASIETIIRDLKGVRARLGESVGTLQQQLLSEATRLQELRG
                     EAEEHTTHLKTLHGIEVDDDTLRTLVRKYQETQKAADESYAARKQGYEAELEAKKAAW
                     RKEQDE"
BASE COUNT          662 a         1324 c         1469 g          538 t
ORIGIN      
        1 gaggtcgttg cggtacggga ccacgccgcc cttggggccg cggacctcct cgcgcacgat
       61 gccggacagg tggaccgtgt cgccgggctt gtagggctcg cggtcggtga agaagaaggc
      121 gcgcggctcg ttgtccccgc ccccgccctg ccagttcgac cagctgccgt cgaggctgcc
      181 ctgcaggggc acgtaggcga cgtcgtcctt ggtcgtcgcg agcaggtagg gctgatcgta
      241 gatcacgccg tgggtcagct cggcgaggcc gcgcgcgtcg gtgcgtccgg tccacgcggg
      301 cgtgtcctgg gcctgcacgt gcagcgccag gtcgacgccg gcgagcggct cgccggtgac
      361 gaggtcggtg acgagtatga cgccgctgtc gctgtcgatc gccgcgctga cgccgaggcg
      421 ggtgacctcg acgaggccgc cgatctgctg gcgatacttc cagtcgccgt cgccgatctt
      481 gttggtgcgc gcgccgaaca gcatcaggtt gccgggcatc gccgcgtggt cgcgcacggt
      541 gagcggcacg gtgaccggct cgacgcgcga gcccgacacg tcggtgacgg tctccttggc
      601 ctgctgcagc ggcagcggcc attgatcgtc gccctgatac tcgttgcgct cgctgagcac
      661 gcggcgcagc tcggccggcg agaccgagcg cgcgcggacc tcgacctgcg tcagaccggc
      721 ggccttgagg tcgagcacac cggcgtgcga gggctcgagc acgatcgggt cgtgcgtcac
      781 gccgtcgacc accagcgagg ggtcgagcgg gcgcagcgtg aaggtcgtgc gaaacgggct
      841 ggcgagggtc tggccgtgga tgtcgcgcac gccggcgtcg acggtcacgg tgtacgtgcc
      901 gagcccgcgg aattttccac ggaggtagat gccaccaccg ccggtgacct cgaggtccgg
      961 gacctcgggc gtgacgtgga ccttgccggg cagctcgggg tcgtcgacga cgaccgtcga
     1021 cgcgccgagc gtgacgccgc cggcgggcga gcagtcccac gcgtaggcct cgttgcagtg
     1081 cggggcgctc agcgtcagcg gcgggtaggt gcgaaacttg ctggtcagcg ccttgctcgg
     1141 gttggggccc tcaccgtagg cgccggcggg cacggtcacg acgtactgcg tgttcggccg
     1201 cagcttgccg ttcgggcgca gcacgagggt ccgctcggcc cggccggcca gcgcctgtga
     1261 cttgtagggc tcgggcaagg tcgcgtagcc ggacaccggc acgagctcga ggccgacgct
     1321 ggtattgcca cccttgagct tgaccgcggc ggcgaggcgc tcgcgctgga tgtcctgatt
     1381 gaacagcagg acgatgtgcg gctcgagctc ggcggtgtcc cactcgctgg gcatcgcgct
     1441 gtcgagcgcg agcaccggcg tcgagaactt ccactgcacg cgcttgccga gcttgttgcc
     1501 gatcgtcgag gcctcgccgg cctcgaccgt ggcggtatag gtggtcgaga acggcacgcg
     1561 accctcggcc tcgaaggcga tcatctgcgt gccgagccag cgaaatttgc cgggcggctg
     1621 cggcgtgatc gacatcggcg agcgctcgag cttgagctcg tcgatcgagg cgagcgggat
     1681 catcggctgg ttgaacacgg ccgtcaccga gccgaccagg cccggctcgg tcatcaccgg
     1741 gctggtgcgc aggacttgca gcggcccggt cgcggccggg tcctgggtct gcttgctcgg
     1801 aggcgcgggc ggagggaacg gcagctcgac gcgctcgctg acgctgggcg ggggctgggg
     1861 acccggcacc ggcctcagct cggtcgtgat gtcgtcgggc agcggcgtga tcggcgggac
     1921 gccggcccac acgtcgagcc cggcgggctg cgtcgcggcg tcggcgggct tgtcgggcgt
     1981 cgcctcgtcg gcgggcttgg tggaggtgca gccgaccagc agcagcgcga cggtccacag
     2041 gctcgcggac aggacagtgg atgtgaagga ggaagagacc gcgactggac gacgttgcat
     2101 gcgagcgctc cgggtgcgcc ggacaacgcc ggtgcgccag cgatgcttac acgggtcgct
     2161 cgcgcggatc cacccgggtg acccgtcgat aggggcgagg atgtcgatga tcacgtcacg
     2221 tcgatcacgt cgatcacgcg gatcacgacg atgtcgacga ccacggtgca cgtacacgcg
     2281 catcgcctga tctccccggg acaaggattg attgatacag tgcatgagct cgcagaccgc
     2341 tccgatgaca cggcgcggcg atactgctgc gagccgccat gcgcgaaccc gcaccgcatt
     2401 cgtatgcgtc gcgggccgtc gctctcgctc gcttgaaaac ctcgttctgc aaaacgcgat
     2461 cgcacgcctc acctggacgc gtgcgctcgc aaggagatgc ccgtgaccag tgtgaacgct
     2521 tccatgtccc gtgacgatgt gttgaggacc tttgccccgt tgctgcacag cttcggccac
     2581 ggccccgcgc cggtggccac caaggcggag gccgccgaac aggccagggc ccgcgagatc
     2641 gtcgatcgtg ccgcttcgta cacgatcgag agcatcgtca agagcctcgc cgacgcgcag
     2701 gtcgagctcg gcggcgcgat cgatggcctc gccgatcagc tcgagcgcga ggcgagcaag
     2761 ctcgatgagc tccgccacgc gctgggctcg gagtcgcgtc ggctcgagga cctgcgaaac
     2821 acgcgggtcg ccgcggaggc gctcgagatc ctgatccagg accacaagaa gaaggacgag
     2881 acgctcgacg cgaaggcgac gtcggagcgc ctcgacctcg agaccaaagt cgccacgcag
     2941 cactccgcgt gggcccgcga ggaggccgag cgctcgcagg cgcgggccga ggcggaggcc
     3001 gcccaggcgc gcgagcgcaa ggcgaaggag gagcagtaca cctacgagat cgagcgccag
     3061 cgcaagctga gcaccgatga gttcgcggag aagaagcgcc tgctcgagcg cacgctctcg
     3121 gagaccggcg cggcgaagga caagaactgg ggcgagcgcg agacgctgct cgcggcggcg
     3181 agcggcgaga tcgaggcgct gcgcgccagg gccgaggcgg cgccgaagca gctcgaggac
     3241 gagaccagga aggcgcgcga gcgggcgatc gcccgcatca ccgcggaggc caagcacgag
     3301 gcggacctgc aggagcgcga gagcgcggcc aacatcgagg tcttcgagct gcggatcaag
     3361 gcgctcgagg agcgcatcgg tcgccagctc aaccagctcg ccgacctcca gcgccagctc
     3421 ggcacggcgc tcgagcaggg acaaaacctc gcccacaagg cgatcgaggg cacctcgggg
     3481 gggaagaagt aaatcatggc caccaagacc tttaccagcc gcagcacgaa gaccgagatc
     3541 ctcaaggcct acaacgagct cgccaaggag ctcaagcagg gccgcggcgc ggcgcctgcc
     3601 gagtctcgcg cgagcacatc ttctgcatca tctgcatcct ccaccgccga cgccgacgag
     3661 cccaccgtga acaccgcatc cgcctccatc gagaccatca tccgcgacct caagggcgtg
     3721 cgcgcccgcc tcggcgagtc cgtcggcacg ctgcaacagc agctgctcag cgaggcgacg
     3781 cgcctgcagg agctgcgcgg cgaggccgag gagcacacga cgcacctcaa gaccctgcac
     3841 gggatcgagg tcgatgacga cacgctgcgc accctggtgc gcaagtacca ggagacgcaa
     3901 aaggccgccg acgagtcgta cgcggcgcgc aagcagggct acgaggcgga gctcgaggcc
     3961 aagaaggcgg cgtggcgcaa ggagcaggac gag
//
LOCUS       DASWNJ010002009         1773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_632234, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002009 DASWNJ010000000
VERSION     DASWNJ010002009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632234"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VGB85_33490"
     CDS             complement(<1..526)
                     /locus_tag="VGB85_33490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139065.1"
                     /translation="MTTIFTGRRISSGLSLLAGLLLGALLMFMGLAKAGRLAPARPEV
                     LVALAQTLEVEHQRIDLLLAQKDVAGAIAALEALRKLEWPERAQAGDAGEQLRHDVYG
                     RLLRLRLDHPKVDPRTPDQLQQIADEGLGDDYKALETNAFTARIVALRGEIAELRGDD
                     GAALTAYEEALTMNR"
     gene            complement(523..1059)
                     /locus_tag="VGB85_33495"
     CDS             complement(523..1059)
                     /locus_tag="VGB85_33495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139066.1"
                     /translation="MSSRNAQPTPDTKRAESVLDPAWEDALRAGQAEDGESGSAGSVD
                     AELGVIHLLRHARAPEPLDPAALDRLWDVEIAPAITPLPWWRRRWLWGVVPAAATAAL
                     LIVVLRSGGDEAFVAERSAAAPGGTLVASASSARALEQQFAQLEGDARRELGVRVDRG
                     RGVMRSQLLAMASAGDKP"
     gene            complement(1156..>1773)
                     /locus_tag="VGB85_33500"
     CDS             complement(1156..>1773)
                     /locus_tag="VGB85_33500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY0139067.1"
                     /translation="RDTLRPVGRREPMPDEQAVVARAASGDRRAFEQIYRAYGPTLFS
                     YVLVPMLGDRDDAHDCLRETFLAAYRHLATFTWHEGGLYPWLKTLAKNKARDLLRASG
                     RRQRLRGAFEEHLGTAGSDEPKNPAEEELQRGALRVRIESVLAELNPRYAQVLRLRLL
                     EDRSREECAAALEVKVGTLDVLLFRACKSFRAACDKQGLVVATEL"
BASE COUNT          234 a          625 c          674 g          240 t
ORIGIN      
        1 cgcggttcat cgtcagcgcc tcctcgtagg cggtgagggc ggcgccgtcg tctccgcgca
       61 gctcggcgat ctcgccgcgc agggcgacga tgcgcgccgt gaaggcgttg gtctcgaggg
      121 ccttgtagtc gtccccgagg ccctcgtcgg cgatctgctg cagctggtcc ggcgtgcgcg
      181 ggtcgacctt cggatgatcg aggcgcaggc gcagcagccg gccgtagacg tcgtgacgca
      241 gctgctcgcc ggcgtcgccg gcctgggcgc gctcgggcca ctcgagcttt cgcagggcct
      301 cgagcgcggc gatcgccccg gcgacgtctt tttgggcgag caacaggtcg atgcgctggt
      361 gctcgacctc gagggtctgg gccagcgcga cgagcacctc cgggcgggcc ggcgcgaggc
      421 ggccggcctt ggccagcccc atgaacatga gcagggcgcc cagcagcagc ccggcgagca
      481 gggacagccc cgaggagatc cggcggccgg tgaagatcgt tgtcatggct tgtctccggc
      541 gctcgccatc gccagcagct gcgaacgcat cacgccgcgc ccgcggtcga cgcggacgcc
      601 gagctcgcgg cgggcgtcgc cctcgagctg ggcgaactgt tgctcgagcg cgcgggccga
      661 cgaggcactg gcgacgagcg tcccgccggg cgccgcagcg ctgcgctcgg cgacgaaggc
      721 ttcatccccg ccgctgcgca gcacgacgat cagcagcgcc gcggtcgcgg ccgccggcac
      781 gacgccccac aaccagcgcc gccgccacca cggcagcggc gtgatcgcag gcgcgatctc
      841 gacatcccac aggcggtcga gcgccgccgg gtcgaggggc tccggggccc gcgcgtggcg
      901 cagcagatgg atcacgccga gctcggcgtc gactgaaccg gcgctcccgg actcgccgtc
      961 ctcggcctgg ccggcccgca gcgcgtcctc ccaggccggg tccaggacgg actcggcgcg
     1021 cttggtgtct ggtgtgggct gggcgttacg cgaggacatg aggaccccca tggacggtgg
     1081 gtatggcggg cgggcggact gcggcctgaa cgtggcccgc acgcgaatgc ttacaagggt
     1141 ggcggcttgt tcggttcata gctcggtggc gaccacgaga ccctgcttgt cacaggcggc
     1201 ccggaagctc ttgcaggcgc gaaacagcag cacgtcgagc gtgccgacct tgacctcgag
     1261 cgccgcggcg cactcctcgc ggctgcggtc ctcgagcagc cgcaggcgca ggacctgggc
     1321 gtagcgcggg ttgagctccg cgagcaccga ctcgatgcgg acccgcagcg cgccccgctg
     1381 cagctcctcc tcggccgggt tcttcggctc gtcggagccg gcggtgccga ggtgctcctc
     1441 gaacgcgccg cgcagccgct ggcgccgccc ggaggcccgc agcaagtccc tggccttgtt
     1501 cttggcgagg gtcttgagcc agggatagag cccgccctcg tgccaggtga aggtcgcgag
     1561 gtggcgatag gccgcgagga aggtctcacg cagacagtcg tgggcgtcgt cgcggtcgcc
     1621 gagcatcggc acgagcacgt acgagaacag cgtcggcccg taggcgcggt agatctgctc
     1681 gaaggcgcgg cggtcgccgg acgcggcgcg ggcgaccacg gcctgctcgt cgggcatcgg
     1741 ttcgcggcga cccacgggcc ggagggtatc gcg
//
LOCUS       DASWNJ010002010         9301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_347725, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002010 DASWNJ010000000
VERSION     DASWNJ010002010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 9301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9301
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347725"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="VGB85_33505"
     CDS             complement(<1..829)
                     /locus_tag="VGB85_33505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEY0139068.1"
                     /translation="MSVMPAVLRDLQAEIKSYADEFGLDTFPVRFEPVDYRHMCEIAA
                     YDGFPTRYPHWRFGMEYDHMLKSHTYGLSKIYELVINTNPCYAYLLEGNSLVDQKLVM
                     AHVYGHCDFFKNNYYFSHTDRRMIDVMANNASRIRRYMDRHGVSTVESFIDVCLSLDN
                     LIDPYLPFRDKQARRPQSSSSNTSEPPTEEEVPAGRLHNVRGYLEGYINPPEFIAAQK
                     AKKKQQEEIAKRKLPAAPERDVLGFLMQHAPLDSWEADVVGILREESYYFLPQRQTKI
                     "
     gene            complement(829..1941)
                     /locus_tag="VGB85_33510"
     CDS             complement(829..1941)
                     /locus_tag="VGB85_33510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HEY0139069.1"
                     /translation="MISRIEQDHSRFREIIRGKIRQNLRKYISRGDMVAPKGQGSVTI
                     PMPQINIPRFVFGDNSGQGVGQGEGDAGDPVGGSPKDGQSGSGKAGEGEGSKALEVEV
                     SLDELAEIMGEELGLPNIQRRGTQSLETKKDRYVGLRTTGPESLRSFKATFKRALRRQ
                     VAMGTYDPKRPVIVPIREDRRYRSWRTDPRPQSNAVIIYMMDVSGSMGDEQKEMVRIE
                     SFWIDTWLRSQYKGLESRYIIHDATARVVDREVFFRTRESGGTMISSAYKVCGELIDA
                     EFNPEQWNVYAFHFSDGDNWSVDDTATCIGLLKNHLLPRLNQFGYGQVESPYGSGQFI
                     KDLRAAFNKHPLLITSEIKTKDDIMDSIREFLKGGR"
     gene            2060..4111
                     /locus_tag="VGB85_33515"
     CDS             2060..4111
                     /locus_tag="VGB85_33515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HEY0139070.1"
                     /translation="MSNIRLVDRIAALQDQKRYEDLHWSGSFEDYLEIARRNPRVART
                     AYERLYDMVLSHGTEEYVDHKKKITRYRFFQDEAHQGRDAIFGLDIPLMRLVNVLKAA
                     ALRYGTERRIILLHGPVGSSKSTIARLLKKGLEEYSRTPEGALYTYEWVLPENLRYLT
                     AGQECFPSPMNEEPLKLIPPEVREQALASLGLEVDGFTPFIRGQLNPACRFIFRELML
                     HHKGDWSKVVQHIRVRRLLLSEEDRVGIGTFQPKDEKNQDSTELTGDINYRRIAEYGS
                     DTDPRAFNFDGEFNVANRGLIEFVEILKLDVAFLYDLLGATQEQKVKPKKFAQTDIDE
                     VIIGHTNEAEYNRLLENKFMEALRDRTVKIDIPYISKLSEEIKIYAKDYNAAKVRGRF
                     IAPHTLEMASMWAILTRLEDPKKHQLSLLQKLKLYDGKTMPGFTQDSVKELRKEANRE
                     GMEGISPRYIQDKIANCLVSDRGQMSINPFMVLNELESGLRNHSLLTSEEHRKRYTEL
                     LAVVKQEYEDIVKNEVQRAISADADAIARLCGNYIDNVKAYTQREKVKNPYTGQDEEP
                     DERLMRSIETKIDIPESRKDDFRREIMNYIGALAIEGKQFDYRTNERLYKALELKLFE
                     DQKDSIKLTSLVSQVVDREAQEKIDVVKQRLIRNYGYDEISATDVLTYVASIFARGEM
                     K"
     gene            4186..4857
                     /locus_tag="VGB85_33520"
     CDS             4186..4857
                     /locus_tag="VGB85_33520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139071.1"
                     /translation="MTYPDLVRNLRSLCCVLLASCGPETAMTEGSDTELTSTGSTSDA
                     TPTTSTAATVSGDDSGTSSTTDLTTGDETTGGTTGPACLQIDHGGDPGQWPEQPEFTC
                     GLSPTCPGEGTVVFKPTFPGSTTNDIDRARCMAAALRDRTPGLLEFEESGTVHTLEVL
                     GEQVIRREEYEGIGFGYSWFEWVEPLQPPEFFADCAEGTASEVRDCLIFATTDECVET
                     FECPG"
     gene            complement(4862..5647)
                     /gene="xth"
                     /locus_tag="VGB85_33525"
     CDS             complement(4862..5647)
                     /gene="xth"
                     /locus_tag="VGB85_33525"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY0139072.1"
                     /translation="MTATAPFSLLTWNINGVRARLDAVLTYLAEREPDIVCLQEIKIE
                     EKVFPRVPFMELGYQLQVHGSKGYAGVATLSKQKPDEVIKGFRAAPLDSHCRILNASI
                     GGVRVYNLYVPNGTSLGSEAFAYKLQWLGRLRDELDASSDPQQPVILCGDFNIAPDAR
                     DLVDPASMVGHTHFSPEEHAALARLLDFGLRDCFRKFNDQPAQYTWFDYRAGSFRKNL
                     GMRLDHVYATAPLYERCTEVVHDASPRGDEGASDHIPVLAKFA"
     gene            complement(5660..7033)
                     /locus_tag="VGB85_33530"
     CDS             complement(5660..7033)
                     /locus_tag="VGB85_33530"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY0139073.1"
                     /translation="MQGAEREASVAEPKKLGRWASHSIVSGSMLGIGIFIFPPVVAQH
                     IHSSGYFMLMWLLGGLAALCGALCVAEMGAMMPRAGGEYPYLRLAYGPGIAFSTGWLQ
                     LLATFPGSLATMAVGAATYQLPVLFGPRMAAPIDLGVMTIAAPTFWAIVLVVLLTALN
                     HIGVVLSGRVQLVLTVVPLAVLLAVSLFVVGDIGTVEVGSAGIDLDLWPIPSAAALAA
                     AYLPVYFAYSGWNAAIYVGGEIEDPGRNLPHAVIGGTLSVLVLYMVLSAGFLSVFSLA
                     DLAGVGEAGTAAAGRLFGQAGIFVMTTMILLAMLGSINSSVMTGSRVGYAMAEQGHFP
                     RAGFRLHPRFGTPIFALWTQTGLAVGLITMQGFEALMRYTSCAMLISGSLTVYAVVRL
                     RERLPELQRPYRVGFYPWTPLAYIGSSLFVLAVLVAQLDVSVFMAVGWFALALLVHHL
                     VVRGRLR"
     gene            7165..8121
                     /gene="bamD"
                     /locus_tag="VGB85_33535"
     CDS             7165..8121
                     /gene="bamD"
                     /locus_tag="VGB85_33535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEY0139074.1"
                     /translation="MPRPRTWRLVWTLAAATAATAASACKTGPDLSTDYGQTARENYE
                     LALGEFEDRDWDEAIAFADFVRIRFPFSRYAVEAELLVARSEFEQGNYLTAQDGFKQF
                     YKLHPTHKHVQNGWCAYMAAVSAYMNAPEGMALLPPHYQRDQAQLQEALVELAYFFDH
                     YAGSSLEPYALELRDEIKRRLLEHELYVARFYLDREVPEAAIGRLEGAHKNFPGIGLD
                     AEVLFLLGLTYLRMDEIELARSTFSELQNQHPRHHHGRQARLYLKYIYKNFGPADANR
                     KRPDRSPPRPISPPRPKQKDEDEAKRKSKPNASLPQPGTASF"
     gene            8118..9236
                     /locus_tag="VGB85_33540"
     CDS             8118..9236
                     /locus_tag="VGB85_33540"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HEY0139075.1"
                     /translation="MTSDPVAVSSRPGRLESDRYGHFSWLLDPPLQTADLPGIGGRIR
                     MCPEDFAVHELPAYGPDGRTGAHMLLTLRKRGIGTEDAIVEVARQTGIPRPDIGLAGL
                     KDKDAVTEQWISVPFAAGPKLAGFSHPAIWLGEPTPHGNKLRRGHLHGNRFTLVVREL
                     ATSGEQAQTRVAAKVARIAELGGLDNLYGNQRLGSDGGNLRRGLELLASGARRGRADF
                     LLSAGQSALFNLYLLERRTRGLMRTVLAGDILKKTATGGLFECTEPAVDQARFDSGEL
                     GITGPMFGSKMRAPAPGTAAAALEQEILDLAGISPDALKALGNKVEGTRRPLQLPQIP
                     LQTELALATDTLPPGLRLHFALPPGSYATVLLQELQGP"
BASE COUNT         1646 a         3375 c         2870 g         1410 t
ORIGIN      
        1 tgatcttggt ctgccgctgc ggcaggaagt agtacgactc ctcgcgcaag atgccgacga
       61 cgtccgcctc ccagctgtcg agcggcgcgt gctgcatcaa gaacccgagc acgtcgcgct
      121 cgggcgccgc gggcagcttg cgcttggcga tctcctcctg ctgcttcttc ttggccttct
      181 gcgcggcgat gaactccggc gggttgatgt agccctcgag gtagccgcgc acgttgtgta
      241 gccgtccggc cgggacctcc tcctccgtcg gcggctcgct cgtgttgctc gagctcgact
      301 gcggacgccg ggcctgcttg tcacgaaacg gcaggtaggg gtcgatcagg ttgtcgagcg
      361 acaggcacac gtcgatgaac gactcgacgg tcgacacccc gtggcggtcc atgtaccggc
      421 ggatgcgcga ggcgttgttc gccatcacgt cgatcatcct gcggtcggtg tgcgagaagt
      481 agtagttgtt cttgaagaag tcgcagtggc cgtacacgtg ggccatcacg agcttttggt
      541 cgaccaggct gttcccctcg agcaggtacg cgtagcaggg gttggtgttg atgacgagct
      601 cgtagatctt cgacagaccg tacgtgtggc tcttgagcat gtggtcgtac tccatgccga
      661 agcgccagtg ggggtagcgg gtcgggaacc cgtcgtacgc cgcgatctcg cacatgtggc
      721 ggtagtcgac cggctcgaag cgcaccggga aggtgtcgag gccgaactcg tccgcgtagc
      781 tcttgatctc ggcctgcaga tcgcgcagca ccgccggcat cacgctcatc accgaccccc
      841 tttcaggaac tcgcggatcg aatccatgat gtcgtccttg gtcttgatct ccgaggtgat
      901 cagcagcggg tgcttgttga acgccgcgcg aagatctttg atgaattgcc ccgagccgta
      961 cggcgactcg acttgcccgt agccgaattg gttcaggcgc ggcagcaggt ggttcttgag
     1021 caggccgatg cacgtcgccg tgtcgtcgac cgaccagttg tcgccgtccg agaagtggaa
     1081 ggcgtacacg ttccactgct cggggttgaa ctccgcgtcg atcagctcac cgcagacctt
     1141 gtacgccgag gagatcatcg tgccgccact ctccctcgtg cgaaagaaga cctcgcggtc
     1201 gaccacgcgg gcggtcgcgt catggatgat gtagcgcgac tccagcccct tgtattggct
     1261 gcgcagccac gtgtcgatcc agaacgactc gatgcggacc atctccttct gctcgtcgcc
     1321 catcgagccg gagacgtcca tcatgtagat gatcaccgcg ttgctctgcg ggcgcgggtc
     1381 cgtgcgccac gagcgatagc ggcggtcctc gcggatcggc acgatcaccg ggcgcttcgg
     1441 gtcgtaggtg cccatcgcca cttgccggcg cagggcccgc ttgaaggtgg ccttgaacga
     1501 acgcaggctc tcggggcccg tcgtccgcag accgacgtag cggtccttct ttgtctcgag
     1561 cgactgcgta ccgcgccgtt ggatgttcgg cagcccgagc tcctcgccca tgatctcggc
     1621 gagctcgtcg agcgagacct cgacctcgag cgccttgctg ccctcgccct cgccggcctt
     1681 gccgctgccg ctctgaccgt ccttgggcga gccaccgacc gggtcaccgg cgtcgccctc
     1741 gccctgtccg acaccctggc cgctgttgtc gccgaacacg aagcgcggga tgttgatctg
     1801 cggcatcggg atcgttacag acccctgtcc cttcggcgcg accatgtcgc cccgcgagat
     1861 gtatttgcgc aagttctgcc gaattttccc gcgaatgatc tctcggaagc gactgtggtc
     1921 ctgttcgatg cgcgaaatca tgggtgaccg cgttcccctt ccgagagact ctcgatgcga
     1981 cgacccacag gtggccggtg cgagaggcct agtgtaacat cacgtcccgc tgtcacgcac
     2041 cctcggcagg aactccacga tgtccaatat ccgcctcgtc gaccgcatcg ctgccctcca
     2101 ggatcaaaaa cgctacgagg atctgcactg gtccggcagc ttcgaggact acctcgagat
     2161 cgcccggcgg aacccccgcg tcgcccgtac tgcctacgag cggttgtacg acatggtcct
     2221 gtcgcacggc accgaggagt acgtcgacca caagaagaag atcacccgct accgcttctt
     2281 ccaggacgag gcccaccagg gccgcgacgc gatcttcggc ctcgacatcc cgctgatgcg
     2341 gctcgtcaac gtgctcaagg ccgcggccct gcgctacggc accgagcgcc gcatcatcct
     2401 cctgcacggc cccgtcggct cgtcgaagag cacgatcgcc aggctgctta aaaaaggcct
     2461 cgaggagtac agccgcacgc ccgagggcgc gctctacacc tacgagtggg tgctgccgga
     2521 gaacctgcgc tacctgaccg ccggccagga gtgcttcccc tcgccgatga acgaggagcc
     2581 gctcaagctg atcccgccgg aggtgcgcga gcaggcgctg gcctcgctcg gcctcgaggt
     2641 cgacggcttc acgccgttca tccgcggcca gctcaacccc gcgtgccgct tcatcttccg
     2701 cgagctgatg ctgcaccaca agggcgactg gtccaaggtc gtgcagcaca tccgcgtgcg
     2761 ccgcctgctg ctgagcgaag aggaccgcgt cggcatcggc accttccagc ccaaggacga
     2821 gaagaaccag gactcgaccg agctgaccgg cgacatcaac taccggcgga tcgccgagta
     2881 cggctcggac accgacccgc gcgccttcaa cttcgacggc gagttcaacg tcgccaaccg
     2941 cggcctgatc gagttcgtcg agatcttgaa gctcgacgtc gccttcctct acgacctgct
     3001 cggcgccacc caggagcaga aggtcaagcc caagaagttc gcgcagaccg acatcgacga
     3061 ggtgatcatc ggccacacca acgaggccga gtacaaccgc ctgctcgaga acaagttcat
     3121 ggaggcgctg cgcgaccgca ccgtcaagat cgacatcccc tacatcagca agctgagcga
     3181 ggagatcaag atctacgcca aggactacaa cgcggccaag gtccgcggcc ggttcatcgc
     3241 cccgcacacc ctcgagatgg cctcgatgtg ggcgatcctc acccgcctcg aggaccccaa
     3301 gaagcaccag ctctcgctgc tgcaaaagct caagctctac gacggcaaga cgatgcccgg
     3361 cttcacccag gactccgtca aggagctgcg caaggaggcc aaccgcgagg gcatggaggg
     3421 catctctccg cgctacatcc aggacaagat cgccaactgc ctcgtcagtg accgggggca
     3481 gatgagcatc aaccccttca tggtcctcaa cgagctcgag tccggcctgc gcaaccactc
     3541 gttgctgacc tcggaggagc accgcaagcg ctacaccgag ctgctcgccg tcgtgaagca
     3601 ggagtacgag gacatcgtca agaacgaggt ccagcgggcg atcagcgccg acgccgacgc
     3661 gatcgcgcgg ctttgcggca actacatcga caacgtcaag gcgtacaccc agcgcgagaa
     3721 ggtcaagaac ccgtacacgg gccaggacga ggagcccgac gagcgtctga tgcgatcgat
     3781 cgagaccaag atcgacatcc cggagtcgcg caaggacgac ttccgccgcg agatcatgaa
     3841 ctacatcggc gccctggcga tcgagggcaa gcagttcgac taccggacca acgagcgcct
     3901 ttataaagcc ctcgagctca agctcttcga ggaccaaaag gacagcatca agctgacctc
     3961 gctcgtctcg caggtcgtcg accgcgaggc ccaggagaag atcgacgtcg tcaaacaacg
     4021 cctgatccgc aactacggct acgacgagat ctcggcgacc gacgtgctca cctacgtcgc
     4081 ctcgatcttc gcccgcgggg agatgaagta agcccggact ccccggacaa accccgcccc
     4141 cgccctcggg ggcttttttg cgcgttcacg cagagcgtcc gacccatgac ctaccctgac
     4201 ctggtacgaa atcttcgttc actctgctgc gtcctgctcg cctcctgcgg ccccgagacg
     4261 gccatgaccg agggttcgga cacggagctc acctcgaccg gctcgaccag cgacgccacg
     4321 ccgacgacga gcaccgcggc cacggtcagc ggcgacgaca gcggcacgtc ctcgaccacc
     4381 gacctcacca ccggagatga gacgaccggc gggaccaccg gcccggcgtg cctccagatc
     4441 gatcacgggg gggaccccgg gcagtggccg gagcagcccg aattcacctg tggtttgagt
     4501 ccgacgtgcc cgggagaggg gacagtcgtg ttcaagccga cattccccgg ctccaccacc
     4561 aacgacatcg accgcgcgcg atgcatggcc gcggcgctgc gcgaccggac gccggggctc
     4621 ctggaattcg aggagtcggg gaccgtccac acgctcgagg tcctcgggga gcaggtcatc
     4681 cgccgcgagg agtacgaggg catcggcttc ggctactcct ggttcgagtg ggtcgagccc
     4741 ctgcagcccc cggagttctt cgccgactgc gccgaaggca cggcgagcga ggtccgggat
     4801 tgcctgatct tcgccaccac cgacgagtgc gtcgagacct tcgagtgccc gggctgaccg
     4861 ctcacgcgaa cttcgccagc accgggatgt ggtccgacgc cccttcgtcg ccgcgcgggc
     4921 tcgcgtcgtg caccacctcg gtgcagcgct cgtacaacgg cgccgtcgcg tacacgtggt
     4981 cgaggcgcat gccgaggttc ttgcggaacg agcccgcgcg gtagtcgaac catgtgtact
     5041 gcgccggctg gtcgttgaac ttgcgaaagc agtcgcgcag cccgaagtcc agcaggcggg
     5101 ccagcgccgc gtgctcctcc ggcgagaagt gcgtgtggcc gaccatcgac gccgggtcga
     5161 cgaggtcccg cgcatccggt gcaatattaa aatcgccgca caggatcacc ggctgctgcg
     5221 ggtcgctcga agcgtcgagc tcgtcacgta gacgtccgag ccactgcagt ttgtacgcga
     5281 acgcctcgct gccgagcgac gtcccgttgg gcacgtagag gttgtacaca cgcaccccgc
     5341 cgatcgacgc gttgaggatc cggcagtggc tgtcgagcgg cgccgcgcga aagcctttga
     5401 tcacctcgtc gggcttctgc ttgctcagcg tcgccacccc cgcgtagccc ttcgagccgt
     5461 ggacctgcag ctggtagccg agctccatga acggcacccg cgggaacacc ttctcttcga
     5521 tcttgatctc ctgcagacac acgatgtccg gctcgcgctc ggccaggtag gtcagcaccg
     5581 cgtcgaggcg ggcacggacc ccgttgatgt tccaggtcag cagcgagaac ggcgcggtcg
     5641 cggtcatggg tcgcggagcc taccgcagtc gtccacgaac gacgagatgg tgcaccagca
     5701 acgccagcgc gaaccacccg accgccatga acaccgacac gtcgagctgc gcgaccagca
     5761 ccgccagcac gaacaggctc gagccgatgt acgccagcgg cgtccacgga tagaacccga
     5821 cgcgataggg ccgttgcagc tcgggcaggc gctcgcgcag gcggaccacg gcgtacacgg
     5881 tgagcgaccc ggagatgagc atcgcgcagg acgtgtagcg catcagcgcc tcgaagcctt
     5941 gcatcgtgat cagcccgacc gccagcccgg tctgcgtcca cagggcgaag atcggcgtgc
     6001 cgaagcgcgg gtgcaggcga aagcccgcgc gcgggaagtg gccctgctcg gccatcgcat
     6061 agccgacgcg cgagccggtc atcacgctgc tgttgatcga ccccagcatc gccagcaaga
     6121 tcatcgtcgt catcacgaag atgccggcct ggccgaacag ccggcccgcc gccgccgtgc
     6181 ccgcctcgcc gacccccgcg aggtccgcga ggctgaacac cgacaggaac cccgcgctga
     6241 gcaccatata gagcaccagc accgacagcg tcccgccgat caccgcgtgc ggcaggttcc
     6301 ggcccgggtc ttcgatctcg ccgccgacgt agatcgccgc gttccagccc gagtacgcga
     6361 agtacaccgg caggtacgcc gccgccagcg ccgccgccga ggggatcggc cacaggtcga
     6421 ggtcgatgcc ggcgctgccg acctcgaccg tcccgatgtc gccgaccacg aacaggctga
     6481 ccgcgagcag caccgccagc ggcacgaccg tcagcaccag ctgcacgcgc cccgacagca
     6541 ccacgccgat gtgattgagg gccgtcagca gcaccaccag cacgatcgcc cagaatgtcg
     6601 gcgccgcgat cgtcatcacc ccgaggtcga tcggcgccgc catcctcggc ccgaacagca
     6661 ccggcagctg gtaggtcgcc gcgccgaccg ccatcgtcgc cagcgagccg gggaaggtcg
     6721 ccagcagctg taaccacccc gtcgagaacg cgatacctgg cccataagcc aggcgcaggt
     6781 acgggtactc gccgccggcg cgcggcatca tggcgcccat ctcggcgacg cacagcgccc
     6841 cgcacagcgc ggcgagcccg cccagcagcc acatgagcat gaagtagccc gagctgtgga
     6901 tatgctgcgc gacgaccggc gggaagatga agatgccgat ccccagcatc gacccgctga
     6961 cgatcgagtg ggacgcccat cggcccagct tcttcggttc cgcgaccgac gcctcacgct
     7021 ccgccccctg catgcgccgg gagcctatcg caaacgccgc cgcccgcgac cgccatgcgg
     7081 cccggaggcc acgaccccgc cgaccttccg gtccgatcgg ggtcgtttga cagcgctggc
     7141 cgccacggct agcttcccgc gcgcatgcct cggccgcgca cgtggagact ggtctggacg
     7201 ctcgcagctg cgacggccgc gaccgccgcg agcgcctgca agaccggccc cgacctcagc
     7261 accgactacg ggcagaccgc ccgcgagaac tacgagctgg cgctcggcga gttcgaggac
     7321 cgcgactggg acgaggcgat cgccttcgcc gacttcgtgc ggatccgctt cccgttcagc
     7381 cgttacgccg tcgaggccga attgctggtc gcccgctcgg agttcgagca gggcaactac
     7441 ctgaccgccc aggacggctt caagcagttc tacaagcttc acccgacgca caagcacgtg
     7501 caaaacggtt ggtgcgcata catggcggcc gtctcggcct acatgaacgc gcccgagggc
     7561 atggccctgc tgccgcccca ctatcaacgc gaccaggccc agctccagga agccctcgtc
     7621 gagctggcct acttcttcga tcactacgcc ggcagctcgc tcgagcccta cgccctcgag
     7681 ctgcgcgacg agatcaagcg ccgcctgctc gagcacgagc tgtacgtcgc ccgcttctat
     7741 ctcgaccgcg aggtccccga ggccgccatc ggccgcctcg aaggcgccca caagaacttc
     7801 cccggcatcg gcctcgacgc cgaggtgctg ttcctgctcg gcctgactta cttgcgcatg
     7861 gacgagatcg agctcgcgcg cagcaccttc agcgagctcc agaaccagca cccgcgccac
     7921 caccacggtc gccaggcccg cctctacctc aagtacatct ataagaattt cggcccggcc
     7981 gacgccaacc gcaaacggcc ggaccgcagc ccgccgcggc cgatctcccc gccgcggccc
     8041 aagcagaagg acgaggacga ggccaagcgc aagagcaagc ccaacgcctc gctcccgcag
     8101 ccagggacgg ccagcttttg acctccgatc ccgttgccgt ctcctcccgc cccggccggc
     8161 tggagtccga tcgttacggc cacttttcat ggctgctcga cccgccgctc cagacggccg
     8221 acttgccggg catcggcggc cggatccgga tgtgcccgga ggacttcgcc gtccacgagc
     8281 tcccggccta cggccccgac ggccggaccg gcgcgcacat gctcctgacc ctgcgcaagc
     8341 gcgggatcgg cacggaggac gcgatcgtcg aggtcgcgcg ccagaccggc atcccgcgcc
     8401 ccgacatcgg cctcgccggc ctcaaggaca aggacgcggt caccgagcag tggatctccg
     8461 tgccgtttgc ggccgggccg aagctcgccg gcttctccca cccggccatc tggctcggtg
     8521 agcccacccc tcacggcaac aagctccgcc gcggccacct ccacggcaac cgcttcaccc
     8581 tcgtcgtccg cgagctcgcc acctccggcg agcaggccca gacccgcgtc gccgccaagg
     8641 tcgcccgcat cgccgagctc ggcggcctcg acaacctcta cggcaaccag cgcctcggca
     8701 gcgacggtgg caacctgcgc cgcggcctcg agctgctcgc cagcggggcc cgccgcggcc
     8761 gggccgactt cctgctcagc gccggccaga gcgccctgtt caacctctat ttgctcgagc
     8821 gacgcacccg cggcctgatg cgcaccgtgc tcgccggcga cattttaaag aagaccgcga
     8881 ccggcggcct gttcgagtgc acggagcccg cggtcgacca ggcccgcttc gactctggcg
     8941 agctgggcat caccggaccg atgttcggca gcaagatgcg cgcccctgcc cccggcaccg
     9001 cggcagccgc gctggagcag gagatcctcg acctcgcggg gatctccccg gacgccctca
     9061 aggcgctcgg caacaaggtc gagggcaccc gccggcccct gcagctcccg cagatccccc
     9121 tgcaaaccga gctcgcactc gccacggaca ccctcccgcc cggccttcgc ctgcacttcg
     9181 cgctccctcc gggtagctac gcgaccgtgc tcctgcaaga acttcagggc ccgtgaacta
     9241 atcccgcgcc cgccgcggta cctccgtcgg caaacgccgc tggccgagtc ggacggtctc
     9301 g
//
LOCUS       DASWNJ010002011         3227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_632299, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002011 DASWNJ010000000
VERSION     DASWNJ010002011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632299"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VGB85_33545"
     CDS             <1..521
                     /locus_tag="VGB85_33545"
                     /inference="COORDINATES: protein motif:HMM:NF018651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139076.1"
                     /translation="IVTSHTYDDRIATIRDAKELGLRTCVGGIFGMGERPEHRAELAM
                     DLRALDVDEVPINFLVSVDGTRLQKVEPMPPLEMLRIIACFRLALPRQNIFIAAGRQH
                     LGQLQPMIFTAGASGMMIGDFLTTANRSVQDDLDMLAQLGLEGTVCGAPRPELARATP
                     RATTVRHLPVMA"
     gene            529..2289
                     /locus_tag="VGB85_33550"
     CDS             529..2289
                     /locus_tag="VGB85_33550"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HEY0139077.1"
                     /translation="MELAQATIFLAAAVLAVPVFKRLGLGAVLGYLAAGVAIGPSGFA
                     FIHEVDQTLHFAELGVVFLMFLVGLELQPARLWKLRAQVFEFGGLQVLVTTLLLAPIG
                     WALGLSPVAAVVAGVALSLSSTAFVLQILAERRSLASPHGQAAFGILLFQDVAASPLL
                     AVLPLLGEARAAAASGGDVWSQVGLALGVIVGLVIAGRYVLGPLFRVVTGTRIRELSA
                     ALALLIVLATAMLMEAVGLSMALGAFLAGVLLADSPYRHELEADIEPFKGLLLGLFFV
                     AVGMSAQLGIVAAQPLLVAGLVLGLVAIKAAVLAVLGRTMFKLPTRGALDLGVSLSQG
                     GEFAFVVCSAAVAAGVFARDLADLLIIVVTLSMVTTPLLFLLRDRLGRETAQQRPFDE
                     IDEPCGVIIAGMGRFGQIVGRVLRMAHVRFTALEVNPTQIDFLRRFGSKIFYGDAARL
                     DLLQAAGAGTAKLLVVAVDDTEAALNIVKHARHQFPQLRILARSRNRQHTHALLAAGA
                     DRVMRETFLSSLETAQHALEELELPDAREIVGAFRDWDERVLSEQFAFRDDEQAMIAH
                     AKKSAEELQRLFETDARSDR"
BASE COUNT          432 a         1236 c         1044 g          515 t
ORIGIN      
        1 agatcgtcac cagccacacc tacgacgacc gcatcgcgac cattcgcgac gccaaggagc
       61 tcgggctgcg cacctgcgtc ggcgggatct tcggcatggg cgagcgcccg gagcaccgcg
      121 ccgagctggc gatggacctg cgcgcgctcg acgtcgacga ggtcccgatc aacttcctcg
      181 tcagcgtcga cggcacgcgc ctgcaaaagg tcgagccgat gccgccgctc gagatgctgc
      241 ggatcatcgc ctgctttcgc ctggccctgc cgcgccagaa catcttcatc gccgccggcc
      301 gccagcacct cggccagctg cagccgatga tcttcaccgc cggcgcctcc ggcatgatga
      361 tcggcgactt tctcacgacg gccaaccgct cggtccagga cgacctcgac atgctcgcgc
      421 agctcggcct cgagggcacc gtgtgcggcg ccccgcgccc cgagctcgcc cgcgcgacgc
      481 cccgcgccac gaccgtgcgg cacctgccgg tgatggcctg acgcggcgat ggagctagcc
      541 caggccacga tcttcctcgc cgcggcggtg ctcgccgtcc cggtgttcaa gcgcctcggc
      601 ctcggcgccg tgctcggcta cctcgccgcc ggcgtggcga tcggcccgtc cggcttcgcc
      661 ttcatccacg aggtcgacca gaccctccac ttcgccgagc tcggcgtcgt gttcctgatg
      721 ttcctggtcg gcctcgagct gcaacccgcg cgcctgtgga agctgcgcgc gcaggtgttc
      781 gagttcggcg gcctgcaggt gctcgtcacc accctgctgc tcgcgccgat cggctgggcc
      841 ctcgggctct cgccggtcgc cgcggtcgtc gccggcgtcg ccctctcgct gtcctcgacc
      901 gccttcgtgc tgcagatcct cgccgagcgc cggtcgctcg ccagccccca cggccaggcc
      961 gcgttcggga tcctgctgtt ccaggacgtc gccgccagcc cgctgctcgc cgtgctgccg
     1021 ctgctcggcg aggcgcgcgc ggccgcggcc agcggcggtg acgtctggtc gcaggtcggc
     1081 ctcgccctcg gcgtcatcgt cggcctcgtc atcgccggcc gctacgtgct cggcccgctg
     1141 ttccgcgtcg tcaccggcac ccggatccgc gagctgtccg ccgcgctcgc gctgttgatc
     1201 gtgctcgcca ccgcgatgct gatggaggcc gtcggcctgt cgatggccct cggggccttc
     1261 ctcgccggcg tgctgctcgc cgactcgccg tatcgccacg agctcgaggc cgacatcgag
     1321 ccgttcaagg gcctgctgct cggcctgttc ttcgtcgccg tcggcatgtc ggcccagctc
     1381 ggcatcgtcg ccgcgcagcc cttgctggtc gccggcctcg tgctcggcct ggtcgcgatc
     1441 aaagcggccg tgctcgccgt gctcggccgc accatgttca aactgcccac ccgcggcgcc
     1501 ctcgacctcg gcgtctcgct gtcccagggc ggcgaattcg cgttcgtcgt gtgcagcgcc
     1561 gccgtcgcgg ccggcgtgtt cgcgcgcgac ctcgccgacc tcctgatcat cgtcgtcacc
     1621 ctgtcgatgg tgaccacccc gctgctgttc ctgctgcgcg accgcctcgg ccgcgagacc
     1681 gcgcagcagc ggcccttcga cgagatcgac gagccctgcg gggtgatcat cgccggcatg
     1741 ggccgcttcg gccagatcgt cggccgcgtg ctccgcatgg cccacgtccg cttcaccgcg
     1801 ctcgaggtca acccgaccca gatcgacttc ctgcgccgct tcggcagcaa gatcttctac
     1861 ggcgacgcgg cccgcctcga cctcctgcaa gccgccgggg ccggcaccgc caagctgctc
     1921 gtcgtcgccg tcgacgacac cgaggccgcc ctcaacatcg tcaagcacgc gcgccaccag
     1981 ttcccacagc tgcggatcct cgcgcgcagc cgcaaccgcc agcacaccca cgccctgctg
     2041 gccgccggcg ccgaccgggt catgcgcgag acctttttgt cgagcctcga gaccgcccag
     2101 cacgcgctcg aggagctcga gctgcccgac gcccgagaga tcgtcggcgc gttccgagac
     2161 tgggacgagc gcgtgctctc cgagcaattc gcgtttcgcg acgacgagca ggcgatgatc
     2221 gcccacgcga agaagtccgc cgaggagctc cagcgcctgt tcgagaccga cgcccgaagc
     2281 gatcgctagc tcttgtgggc gcggtggcgt gtgcgggcgt cgggtggcgt gttcggggga
     2341 gcgcggggcg agactacatg ggccgggggg ccgacaggct gcgggtccct gcgacgcgac
     2401 gcgggtcctc ccaggggaca tgctcgccgc cccatctcgc gcgcatcaca gggtcctctc
     2461 accgaccctt catcgcgacg acgggagttg acggggcgtc tgcgcgtgac ccctgggtcc
     2521 tcccgctgct tcccccgccg gtcggccctt ccccccggaa ccagcactgt ctgcgccccg
     2581 cttgtcgccg gacgaatacc tcgcgcaccc gaacacgagt gctgccataa gctcgacaca
     2641 caaatcagca ctcaatcgct ctctgctcaa agcagcacgc ataatcggac actcgccgcc
     2701 gagctggcca cgtgctccac gactcaccat tcgcgtttct ggcgcgacat gctccgagct
     2761 gaccagcatc gccacagtcc gctgtatgtg ctccaaagcg cgttacgtcc gcgaacccgc
     2821 cgtataagca ctcgagcacg cgaaatgccc gcgctcgcca cccgcgggca cacatcgcgc
     2881 gacacctctc ggtactcgca acgagcgggg tgccgacgat gtccgcccgg ggttgggggc
     2941 cgacgagcgg gggaagcagc gggaggaccc aagggtcacg cgcagacgcc ccgtcaactt
     3001 ccttcgcggc aatgaagggt cggtgagacg atcgcagtgt gcgggacaga tggggcggcg
     3061 agcatgtccc ctgggaggac ccgcgtcgca ctggccgtta gccggaatta tcgtccattt
     3121 gctgcaaggc gagtcgacgg ccgagcacga gtggttcgag gtaagcgagg ccgcgggcga
     3181 tggtgacggc gccgggtgcg accttgctgg acttcatgaa gccgccg
//
LOCUS       DASWNJ010002012         4720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_419522, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002012 DASWNJ010000000
VERSION     DASWNJ010002012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419522"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VGB85_33555"
     CDS             complement(<1..603)
                     /locus_tag="VGB85_33555"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0139078.1"
                     /translation="MTETADPTAIGPFIVLRKLGEGAMGVVYAGFDVSLDRKVALKLV
                     RRQLLDKPTVRARMIREAQAMARLSNPHVVQIYQVGEHAGGIYVAMEYIDGQTLGEWL
                     RARQRTWQAILRTVCEAGRGLAAAHAAGLVHRDFKPDNVLVDTQDHARVLDFGLVQTE
                     ASDVEDVLARTEDDAPVTATGHDGVELSNIHWSVRLTQVGK"
     gene            complement(648..1574)
                     /locus_tag="VGB85_33560"
     CDS             complement(648..1574)
                     /locus_tag="VGB85_33560"
                     /inference="COORDINATES: protein motif:HMM:NF039450.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADYC domain-containing protein"
                     /protein_id="HEY0139079.1"
                     /translation="MATQRAGLVMMAVLLVAGACDAEDASPEVVTARGLELNGFLLNG
                     FLLNGEKLDGAPGTTDYIRITAVDLKGGNKGGTSWLTGSTLAVEGNGGVVFSGAALID
                     ARIEYDLSEGTKGPHKKSVRIVGVEPLAPGSDVMLYELELHDGGWKPLCEGGAKAILL
                     GDVWEPATGDRISGPNDGAVTFACRGAALAKCVEYGYRPWATAGNVSLREYHQACTRM
                     VRADYCGEGLAHTTNGTPIHVVDKVGVQNVDPNVNYVVEAEWNADGATCLNPANTRHP
                     NQVIGCTIPTCNKKTSFAAGGLIQSGKVLAGP"
     gene            2248..3012
                     /locus_tag="VGB85_33565"
     CDS             2248..3012
                     /locus_tag="VGB85_33565"
                     /inference="COORDINATES: protein motif:HMM:NF013988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMMECR1 domain-containing protein"
                     /protein_id="HEY0139080.1"
                     /translation="MEAAAVPAVSSAVAPVPLADSSRRWLLFVARRALRNAMAQRNPG
                     ALDLPVGNAERPDDPCLSTPARVFVSWYDGGERVGSLGSLEPWLPLEQAVARYAVHAG
                     LDPKLPAARASRWHRLVGEISILGAPQDLPVSGLTAIAEALVPGRDGIILTAGPRSVR
                     PASGPLPAVGATSTSSSTSSPSRQQAVSLPSAWRQSPRARDFVVALMRTAGLSPEHDG
                     PRLRARTFLAETFVEPVAAVMPAAVSRVAPELHLGV"
     gene            3250..3894
                     /locus_tag="VGB85_33570"
     CDS             3250..3894
                     /locus_tag="VGB85_33570"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEY0139081.1"
                     /translation="MRMILIGPPGAGKGTQAQSLVTTFGIPHISSGDMLREAVSAGTL
                     LGKTAAGFMKEGLLVPDDVVIGMVIERIGREDCAKGFMLDGFPRTLPQAEALDVAMAR
                     SQVELDAVVLLEVGDELILERVTGRRTDPVTGNIYHLKFKPPPPEAVERLVHRSDDFE
                     AACSARLEKYHRETTPIVPFYERKGLLRRVDGVGLPDEVLMRIVAALQTVAAAS"
     gene            3912..>4720
                     /locus_tag="VGB85_33575"
     CDS             3912..>4720
                     /locus_tag="VGB85_33575"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY0139082.1"
                     /translation="MTNSKIRYWAPQIATLGAMLCGFLSIVAATQGRFADAGWLILLS
                     TFLDRADGLLARSLHATSDFGVQMDSFADFFDFGVAPAVLLFIALTGEPALGYTSGTG
                     YALVVAACGFWIAAATFRLARFNVLTDTAPDKNLFNGIPTTLAAGLLVNWFLVCLKYA
                     APGNPLGAADAFTEPRLFGGLQLGPGAWSVFPIAMLVGGLLMVSNLRCRKFLLFKNKV
                     GSVTVLLLVLGAGVCLLLRVFPEYMVTLPTMWISFWLVYGQVAPKYRTLASP"
BASE COUNT          630 a         1696 c         1578 g          816 t
ORIGIN      
        1 cttgccgacc tgcgtcaggc gcactgacca gtggatgttg gagagctcga ccccatcgtg
       61 acccgtcgcg gtgaccggcg cgtcgtcctc ggtgcgcgcc aggacgtcct cgacgtcgct
      121 cgcctcggtc tgcacgaggc cgaagtcgag gacacgcgcg tggtcctggg tgtcgacgag
      181 gacgttgtcg ggcttgaagt cgcggtgcac caggcccgcg gcgtgggccg ccgccagccc
      241 gcgcccggcc tcgcagacgg tgcgcaggat cgcctgccag gtccgctggc gggcgcgcag
      301 ccactcgccg agcgtctgcc cgtcgatgta ctccatggcg acgtagatgc cgcccgcgtg
      361 ctcaccgacc tgatagatct ggacgacgtg cgggttggac aggcgggcca tcgcctgcgc
      421 ctcgcggatc atccgggcgc gtacggtcgg cttgtcgagc agctgccggc ggaccagctt
      481 gagcgcgacc ttgcggtcga ggctgacatc gaagccggcg tacacgaccc ccatcgcccc
      541 ctcgcccagc ttgcgcagga cgatgaaggg gccgatcgcg gtcgggtctg cggtctccgt
      601 catgattcag attcagtgga tgcggtcgat ggggtcgatg cgggccctca ggggccggcg
      661 aggaccttgc cgctctggat caggccgccc gcggcgaatg aggtcttctt gttgcaagtc
      721 gggatcgtgc agccgatcac ctggttgggg tggcgggtgt tggcggggtt gaggcaggtc
      781 gcgccgtcgg cgttccactc ggcctcgacg acgtagttga cgttcggatc gacgttttgc
      841 acgccgacct tgtcgaccac gtggatcggc gtgccgttgg tcgtgtgcgc cagaccctcg
      901 ccgcagtagt cggcgcggac catccgcgtg cacgcctgat gatactcgcg cagtgacacg
      961 ttgccggcgg tcgcccacgg gcggtagccg tactcgacgc acttcgccag cgcggcgccg
     1021 cggcaagcga aggtcaccgc cccgtcgttg ggcccggaga tgcggtcgcc ggtcgccggc
     1081 tcccagacgt cgccgagcag gatcgccttc gcaccgccct cacacagcgg cttccacccg
     1141 ccgtcgtgga gctcgagctc gtagagcatg acgtcggagc ccggcgcgag cggctcgacg
     1201 ccgacgatcc gcaccgactt tttatggggg cccttggtgc cctcgctcaa gtcgtactcg
     1261 atgcgcgcgt cgatcagggc cgcgccggag aagacgacgc cgccgttccc ctcgacggcg
     1321 agcgtgctgc cggtgagcca gctcgtgccg cccttgttgc cgcccttcag atcgaccgcg
     1381 gtgatcctta tgtagtcggt cgtgccgggc gctccgtcga gcttctcccc gttgagcagg
     1441 aagccgttca acaggaaccc gttgagctcg agcccacgag cggtgaccac ctctgggctc
     1501 gcgtcctcgg cgtcacaggc ccccgccacc agcagcaccg ccatcatcac aagccctgct
     1561 ctttgcgtcg ccatgatcac cctccgcgag tcgtctcgtc gggactatac caacgcagag
     1621 tgacgaacgc cgcattcgtg acgcgccgcg cgatgtaaga ccatgtcctc atcggtcaga
     1681 cagacccctg ggttcccctc gatccggacc gtcctgctgg gcaaaaagcg tggcgtgttc
     1741 gcgtgttcgc gcgtccgcga cgctgttcga cgcgacgagg gtgcgagacc cgatgcgaag
     1801 attctgcggc agcaacttcg atggatcgtg gtccccaggc ccgacatgtc ggcgggaagc
     1861 cacgctcctt cgttgccgga gcatcgatcg ccagacttcg cccgacgctg cggtgcggcg
     1921 cctcgaggtc gatgaagtga ccacgtgaag ctccgcgagc ggtcggtgtg ccgatacaat
     1981 gatatgtcga tcgcagtacc gaggtctcga gtcgtcgacc gaataccgag gtggcaccgg
     2041 agcagcactg cgagtcgtat cgacgtcacg tcgcgagcat gcgaggcgct gggcgcctcg
     2101 cgtcggccgg ccttcgccgc ggtccggggt gacggccacc tgtccgcgac aaccagcgag
     2161 ctagcgcatg gaattcgcgg agggagagcg caagatagtg cttttcaaag cggcgcgggt
     2221 cggtcatctt acgcgccgct aagattcatg gaagccgccg ccgtccccgc tgtctcgtcc
     2281 gctgttgccc ccgtgccgct cgccgattcg tctcggcgct ggctgttgtt cgtggcccgt
     2341 cgtgccctgc gcaacgccat ggcgcagcgc aatccgggtg cgctggacct gccggtcggc
     2401 aacgccgagc gtcctgacga cccgtgcctg tcgacgccgg cccgcgtgtt cgtctcctgg
     2461 tacgacggcg gcgagcgggt cggcagcctc ggctcgctcg agccgtggtt gccgctcgag
     2521 caggcggtgg ctcgctacgc ggtgcatgcc ggcctcgacc cgaagctacc ggcggcccgg
     2581 gcctcccgct ggcaccgcct cgtcggtgag atctccatcc tcggcgcgcc gcaggacctg
     2641 ccggtcagcg gcctcaccgc gatcgcagag gcgctggtgc ccgggcgtga cggcatcatc
     2701 ctcaccgccg gcccgcgtag cgtccgtccg gcctcgggtc cgctgcccgc ggtcggtgcg
     2761 acctctacga gctcgagcac ctcctcgccc agccgccagc aggccgtctc cctgccctcg
     2821 gcgtggcgtc agtcgccgcg cgcccgcgac ttcgtggtcg ccctgatgcg tacggccggg
     2881 ctctcgccgg agcatgacgg tccgcgcctg cgtgcccgca cgttcctggc agagaccttc
     2941 gtcgagccgg tggccgcggt gatgccggcg gccgtgagcc gcgtggcgcc cgaattgcac
     3001 ctcggcgtct gagccgggcc ctgctgttgc acctgtcgtc cacctgtcgt ccacctgtcg
     3061 tccacctgtc gtcttggcgg ccgcccggac cgcgggtgac gggtgcgtga tcgccggaga
     3121 ccggccttcg atcttgcgag cttgcgcggc ggcatgccac actgcgcccg ccggtcgttc
     3181 ccgcgctttt tcgaccgcct gatcgtctcc ctggccgcac ggcctgattt ctcgccgcga
     3241 gatccatata tgcgaatgat cctcatcggc cccccgggcg ccggcaaagg cacgcaggcg
     3301 cagagcctgg tcaccacctt cggcatcccg cacatctcgt cgggcgacat gctgcgcgag
     3361 gccgtgagcg ccgggaccct gctcggcaag acggcggcgg gcttcatgaa ggagggcctg
     3421 ctggtccccg acgacgtcgt catcggcatg gtgatcgagc gcatcggtcg cgaggactgc
     3481 gcgaagggct tcatgctcga cggcttcccg cgcacgctcc cgcaggccga ggcgctcgac
     3541 gtggcgatgg cgcgctcgca ggtcgagctc gacgcggtgg tgttgctcga ggtgggcgac
     3601 gagctgatcc tcgagcgcgt caccgggcgc cgcacggacc ccgtgaccgg aaacatctac
     3661 cacctcaagt tcaagccgcc gccgccggag gcggtcgagc ggctggtcca tcgcagcgac
     3721 gacttcgagg ccgcctgctc ggcgcgcctc gagaagtatc accgcgagac caccccgatc
     3781 gtcccgttct acgagcgcaa gggcctgctg cggcgggtcg acggcgtcgg cctgcccgac
     3841 gaggtcctga tgcgcatcgt cgccgcgctg cagaccgtcg cggcggcgag ttgacgggcc
     3901 ggcaagcacg catgaccaac agcaagatcc gctactgggc cccacagatc gccacgctcg
     3961 gcgcgatgct gtgcggcttc ctgtcgatcg tcgccgccac ccaggggcgc ttcgccgacg
     4021 ccggctggct gatcctgctg tcgaccttcc tcgaccgcgc cgacggcctg ctcgcgcgct
     4081 cgctgcacgc caccagcgac ttcggcgtgc agatggactc gttcgccgac ttcttcgact
     4141 tcggcgtcgc cccggcggtg ctgctgttca tcgccctcac cggtgagccc gccctcggct
     4201 acaccagcgg caccggctac gcgctcgtcg tcgccgcctg cgggttctgg atcgcagcgg
     4261 cgacgtttcg cctcgcgcgc ttcaatgtcc tcaccgacac cgcgcccgac aagaacctgt
     4321 tcaacggcat cccgacgaca ctcgccgccg gactgctcgt caactggttc ctcgtctgcc
     4381 tcaagtacgc cgcgccgggc aacccgctcg gagccgccga tgccttcacc gagccgcgcc
     4441 tgttcggcgg cctgcagctc gggcccggag cgtggtccgt gttcccgatc gcgatgctcg
     4501 tcggcggcct tttgatggtc tcgaacctgc gctgccgcaa gttcctgctg ttcaagaaca
     4561 aggtcggctc ggtcactgtc ctgctgctgg tcctcggcgc aggcgtgtgc ctgctgctgc
     4621 gcgtgttccc ggagtacatg gtcacgctgc cgacgatgtg gatctcgttc tggctggtct
     4681 acggccaggt cgccccgaag taccgcaccc tcgcgtcccc
//
LOCUS       DASWNJ010002013         2904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_347981, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002013 DASWNJ010000000
VERSION     DASWNJ010002013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347981"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1213
                     /locus_tag="VGB85_33580"
     CDS             158..1213
                     /locus_tag="VGB85_33580"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139083.1"
                     /translation="MKIAITYNADTHLKPHLNEVERLGEDEVAESARDVYAALSASHH
                     CELFPVGDCIAAALLAIRAFAPDVVFNLCEGVLGRTRWEAHFVLALDLLGIPYCGCDA
                     VTIALTQDKGFIKQLLRQIGAPTPEGLAVAEGCPEGQVSAQIEAMLARAPSGRVIVKP
                     VREDGGIGVDAASVVADAAAALDRCRMIWEQYQQPALVEVFLDGKEYNLALYASEQGL
                     VTLPPGEIRFAPDLKAEERVVGWKAKWDLGSVEDLSTESRIARDMDPATHAEITATCR
                     HVAAMMGMSGYCRFDLRQGPDGRINIIDINANPDIGPGSGFRKALAAADIRFEDFLEE
                     LIGARLRAQRVPWRSAA"
     gene            1195..1704
                     /locus_tag="VGB85_33585"
     CDS             1195..1704
                     /locus_tag="VGB85_33585"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0139084.1"
                     /translation="MAIRSVSAGDIPRIHAILVSTGVFTPAEIECADELVAEAVHEPH
                     KGDYLVHVIEDDAGVQGYVCFGATPLTEGTYDLYWIAVDPSQHGRGHGQKLVAFVEAW
                     LQEHGGRLLLIETSSKDDYGPTQAFYLRCGYTELARVPAFYRPGDDKIVYGKYLSPGT
                     SDRAGHAVR"
     gene            1790..>2904
                     /locus_tag="VGB85_33590"
     CDS             1790..>2904
                     /locus_tag="VGB85_33590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139085.1"
                     /translation="MLTPHHYKIVQLTEDYAALTRDDRPRFEQILWAIQGEQRRLPGE
                     FGFRVPVAMVLLGGGYRERAIEHVKGSLKGLKSGTMRTFLVLILIEQLTALGMFSDAN
                     RLLVMLLGVGLPESELVKLQHHALSLALRSGSVELLHRCAQAAHGPNDSAKLILERLQ
                     QHGLLEWLSRQQRALEAVLGPVTTHFTHAVLTDDTGAKRFVLTYHTCTPTPDLRALNQ
                     RVFDALHDLYEVHPRGPAAFLGHIVIDVRGPLIAETAPVDQEVSQLKRGDLSVQTLWR
                     GVRRFAQGRPLDPALRRNWTISRVFQAALHQAMAAAPTAGIRLCALATGGPYDQLLAG
                     IVGSLDREWRFRGMCLEQLRCAAILTEYSPGHAATAT"
BASE COUNT          460 a         1065 c          940 g          439 t
ORIGIN      
        1 aatccggtgc acagcgacct cgttatcatc gcgaacggcc atggctggac ttttgaccag
       61 ctgatcggtc ggatcgtcga cgcggcctcc gagcgctggg gacagcgcgc cacctcttgt
      121 tcttaacctc gcgcgcagcc caagacgaac agcgacgatg aagatcgcaa tcacctacaa
      181 cgccgacacc cacctcaagc cgcacctcaa cgaggtcgaa cgcctcggcg aggacgaggt
      241 cgccgagtcc gcccgcgacg tgtacgcggc gctgtcggcc agccaccact gcgagctgtt
      301 cccggtcggg gactgcatcg ccgcggccct gctggcgatc cgcgcgttcg cgccggacgt
      361 ggtgttcaac ctgtgcgagg gcgtgctcgg gcgcacccgc tgggaggccc acttcgtgct
      421 cgcgctcgac ctgctcggca tcccctactg cggctgcgac gcggtgacga tcgccctgac
      481 gcaggacaag ggttttatca aacaactgct gcggcagatc ggggcgccga cgcccgaggg
      541 gctcgcggtc gccgagggct gtcccgaggg acaggtgtcg gcgcagatcg aggcgatgct
      601 ggcgcgcgcg ccgtccggtc gcgtcatcgt caagccggtc cgtgaggacg gcgggatcgg
      661 cgtcgacgcg gcgagcgtcg tcgccgacgc cgccgcggcg ctcgaccgct gtcgcatgat
      721 ctgggagcag taccagcagc cggcgctcgt cgaggtcttc ctcgacggca aggagtacaa
      781 cctggccctc tacgccagcg agcagggcct cgtcaccctg ccgcccggcg agatccggtt
      841 cgcgccggac ctcaaggccg aggagcgcgt cgtcggctgg aaggccaagt gggacctcgg
      901 atccgtcgag gacctctcga ccgagagccg gatcgcccgc gacatggacc cggcgaccca
      961 cgcggagatc acggcgacct gccgccacgt cgccgcgatg atgggcatga gcggctactg
     1021 ccgcttcgac ctgcgccagg gccccgacgg ccggatcaac atcatcgaca tcaacgccaa
     1081 ccccgacatc gggcccggca gcggctttcg caaggcgctc gccgccgccg acatccgctt
     1141 cgaggatttc ctcgaggagc tgatcggcgc ccgcctccgg gcccagcgag tcccgtggcg
     1201 atccgcagcg taagcgccgg ggacatcccc cggatccacg cgatcctcgt ctccaccggg
     1261 gtgttcaccc cggcggagat cgagtgcgcc gacgagctcg tcgccgaggc cgtccacgag
     1321 ccgcacaagg gcgactacct cgtgcacgtg atcgaggacg acgccggcgt gcaaggctac
     1381 gtgtgcttcg gcgcgacccc gctgaccgag ggcacctacg acctgtactg gatcgcggtc
     1441 gacccgagcc agcacgggcg cggccacggg caaaagctcg tcgccttcgt cgaggcctgg
     1501 ctgcaagagc acgggggcag gctgctgttg atcgagacct cgtccaagga cgactacggg
     1561 ccgacgcagg cgttctacct gcgctgcgga tacacggagc tcgcgcgcgt gcccgcgttc
     1621 taccgcccgg gagacgacaa gatcgtgtac ggcaagtacc tgtcgcccgg gacgtcggac
     1681 cgtgctggcc acgccgtccg gtgatcgcgt agaagcgacc ggcgccgcga agacgcgggc
     1741 atctaacgac gcgggcgaaa atgcgctacc ctgaccgccc ccgccggtca tgcttacacc
     1801 tcatcactac aagatcgtcc aactcaccga agactatgcc gcgctgacgc gcgacgatcg
     1861 cccccgcttc gagcagatcc tctgggccat tcagggcgag cagcgccgtc tccccggcga
     1921 gttcggcttt cgtgtgcccg tcgccatggt cctgctcggc ggcggatatc gcgagcgggc
     1981 gatcgaacac gtcaagggtt cgctcaaggg cctcaagtcc gggaccatgc gcaccttcct
     2041 ggtgctcatc ttgatcgagc agctgaccgc gctcggcatg ttctccgacg ccaatcgcct
     2101 gctggtcatg ctcctcggcg tgggcctccc cgagtcggag ctcgtcaagc tgcaacacca
     2161 cgcgctgagc ctcgcgctgc gcagcggctc ggtcgagctg ctgcatcgct gcgctcaggc
     2221 cgcccacggc ccgaacgact cggcgaagct gatcctcgag cggctgcagc aacacggctt
     2281 gctcgagtgg ctgtcgcggc agcaacgggc gctggaggcc gtgctcggtc ccgtgaccac
     2341 ccacttcacg cacgccgtgc tcacggacga caccggggcg aagcgatttg tcctgaccta
     2401 tcacacctgc accccgacgc ccgacctgcg cgcgctcaat caacgggtct tcgacgcgct
     2461 gcacgacctc tacgaggtcc acccacgcgg gcccgccgcg ttcctcggtc acatcgtgat
     2521 cgacgtccgt ggccccctca tcgccgagac cgcgccggtc gatcaggagg tcagtcagct
     2581 caagcgcggc gacctcagcg tgcagaccct gtggcgcggc gtgcgtcgct tcgcgcaggg
     2641 ccgcccgctc gatccggccc tgcgccgcaa ctggacgatc tcacgcgtct ttcaggccgc
     2701 gctccatcaa gcgatggccg cggcgccgac cgccggcatt cgtctctgtg cgctcgccac
     2761 gggcggcccc tacgatcaac tgctcgcggg gatcgtcggc tccctcgacc gcgagtggcg
     2821 ctttcgtggc atgtgcctcg agcagctacg ctgcgctgcg atcctcaccg agtactcccc
     2881 gggccatgcc gccaccgcga ccga
//
LOCUS       DASWNJ010002014         4825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_419675, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002014 DASWNJ010000000
VERSION     DASWNJ010002014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419675"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..1608
                     /gene="hydA"
                     /locus_tag="VGB85_33595"
     CDS             235..1608
                     /gene="hydA"
                     /locus_tag="VGB85_33595"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEY0139086.1"
                     /translation="MSGVVIRNGTIVTALDQYVADIYCEAGIIKAIGVGLDVPSDSQV
                     IDASGQYVFPGGIDAHTHMELPFMGSVSADDFFTGTAAGVAGGTTTILDFIIPNRNQS
                     LIEARDFWMNNAKKSCADYGFHMAVTWYGEQVEQEMRRVHAEDGISSFKVFMAYTGAI
                     GVDDDEMIGVIDTAASLGALVTAHCEHGLAVQALQRKLVAQGKTAPRHHAESRPSWVE
                     GEATNRAIMLAKCAGQPIYIVHLTCKESMDAVVRARQDGQLVYVETCPQYLLLDDSVY
                     DKPDFEGAAYVMSPPIRPIGHQDVLWNALSCGLIHTVATDHCPFRQHDQKTNGRDDFT
                     KIPNGAAGIENRMPLMYTYGVATGRMTLQQFVDTCCTRPAKIFGLYPRKGAILVGSDA
                     DLFLYDPSGGSTISASTHRHNCDRNIFEGFAIKGAVTHTIVAGRLQFKDGDLRVERGA
                     GRYLRRT"
     gene            2114..3037
                     /locus_tag="VGB85_33600"
     CDS             2114..3037
                     /locus_tag="VGB85_33600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139087.1"
                     /translation="MRDEPPPDPPPTIGPGAVEAPEPAPRHRLIYSNTLVLRVNPLGL
                     EDRFSLMYRRRLSARTGKLWDDTYFGVGITPSFAPSITRFGPTVMLVPLAILSLRASY
                     YFVGNYGSQSFKPHPFDSPADEYGPDTIKARADARQGINTYGGQAELSALFQVKFGPI
                     ALRNEVIFFHNSIKLPGSNDVYYDIRHDIMAPGKGWFLSNDTDLLYTNAKLRLNAGVR
                     GTYYHIFYPETLYAPGDIAGTNQNDSARVGPLISYAFKDRPEKRFTKPTLFFAAQWWV
                     KHRYRTGQEVSQGLPLMILGFSFTGELFSRK"
     gene            complement(3009..>4825)
                     /locus_tag="VGB85_33605"
     CDS             complement(3009..>4825)
                     /locus_tag="VGB85_33605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139088.1"
                     /translation="GLVVPQQFAGTVFGLRADSEYEIELHAQDPDGLDETWTVMGRTR
                     PVPADPGQAKTVMVSDAAGLSAALAAAAPGDVISLADGTYSGQFSISASGTAADPIVI
                     RGASTEGTIVDGGGCDGCNVLEIYGSFVHLERLTLQAATRALRFQGAGSEGNVVRRVH
                     IRDVRLGIGAKADQKNFYLCDNVLEGRLAWPAVWGDDGGMFANEDGIVVSGDGHVVCH
                     NRLVGFGDSIKIEQLGARAFDVYGNLSLSAYDNALELDETAGNARAVGNMFLNSWSPL
                     SFQPIFGGPAYAIRNVAVNIVDEQQKLHSNGNLGETVGALLMHNTFVSPRHAVYLQAA
                     ATAHDLRLINNLYVGPAAPENDKVVDWSVPIDDAVLDGNGYYPDGFFDFGGADKWQGF
                     AAMQAAGKFEANGVLLTAGTFASGLQAPASYVETVAEADVTLAADSPAVDAGLPLPGV
                     NSLYLGGGPDIGAHELGCPTPIYGVRPEGVDESDPPASCEEGGDDTGTDTGLETGAQT
                     GSASDPSGSETGSTPTSGGTDGGSSPTGEDGGDGTGSGTGSTTYDTSNDGGQGSDDGG
                     QDSSAGCGCRGGTGPGALWLALLLLPRRRFTSVRTARR"
BASE COUNT          755 a         1898 c         1447 g          725 t
ORIGIN      
        1 cggtgaaacg actctggtaa tctcgacaga tggggcggcg agcacgtccc ctgggaggac
       61 ccgcgtcgca gggacccgcc gtccgtcggc ccctccccgg cccaaacagc ctcgccccgc
      121 gctctcggaa cacgccacac cggcccccga acgagccaca ccggccccgg accgcgcgac
      181 accagccccc gaacacttcc cgtcatcgaa ccattcgccc tcaaggagtc agtcatgtcc
      241 ggcgtagtca tccgcaatgg aaccatcgtc accgccctcg atcagtacgt cgccgacatt
      301 tactgtgagg ctggcatcat caaggcgatc ggcgtcgggc tcgacgtccc ttccgacagc
      361 caggtgatcg acgcctcggg ccagtacgtg ttccccgggg gcatcgacgc gcacacgcac
      421 atggagctgc cgttcatggg cagcgtcagc gccgacgact tcttcaccgg caccgcggcg
      481 ggcgtcgctg gcggcacgac gacgatcctc gacttcatca tcccgaaccg caaccagagc
      541 ctgatcgagg cccgcgactt ctggatgaac aacgcaaaaa aatcctgcgc cgactacggc
      601 tttcacatgg ccgtcacctg gtacggcgag caggtcgagc aggagatgcg gcgcgtgcac
      661 gccgaggacg gcatcagctc tttcaaggtg ttcatggcgt acaccggcgc gatcggggtc
      721 gacgacgacg agatgatcgg cgtcatcgac accgcggcga gcctcggcgc cctcgtcacc
      781 gcgcactgcg agcacggtct cgccgtccag gcgctgcagc gcaagctggt cgcgcagggc
      841 aagacggccc cgcggcacca cgccgagagc cggccctcgt gggtcgaggg cgaggcgacc
      901 aaccgcgcga tcatgctcgc caagtgcgcc ggccagccga tctacatcgt gcacttgacc
      961 tgcaaggagt cgatggacgc ggtcgtgcgc gcgcgccagg acggccagct cgtctatgtc
     1021 gagacctgcc cgcagtacct gctgctcgac gactcggtgt acgacaagcc cgacttcgag
     1081 ggcgcggcgt atgtcatgag tccgccgatc cgcccgatcg ggcaccagga tgtcctgtgg
     1141 aacgccctgt cgtgcggcct gatccacacc gtcgccaccg atcattgccc gtttcgccag
     1201 cacgaccaga agaccaacgg ccgcgacgac ttcaccaaga tccccaacgg cgcggccggc
     1261 atcgagaacc gcatgccgct gatgtacacc tacggcgtcg ccaccggccg catgaccttg
     1321 cagcagttcg tcgacacctg ctgcacgcgc ccggccaaga tcttcgggct ctacccgcgc
     1381 aagggcgcca tcctggtcgg ctccgacgcc gacctgttcc tctacgaccc cagcggcggc
     1441 agcacgatct ccgcgagcac ccaccgccac aactgcgacc gcaacatctt cgagggcttc
     1501 gcgatcaagg gcgccgtcac tcacacgatc gtcgccgggc gcctgcagtt caaggacggc
     1561 gacctgcggg tcgaacgcgg cgcgggccgg tacctccgcc gcacctgaaa ataacgccgt
     1621 ccagccccac gcgcgatggc tgaccttcgg gacaggttcg cgcgccggac attctcgtcc
     1681 tgactgatcg gacagccggg cccgcgcact tgggcctatc gccgcgccgg ccgccgtgcg
     1741 actctcgccg acgatgacgg tcctgcgcgc tcggctcctg tgccctcccc tcctcgccat
     1801 gtgcctcgga ggggctcctg tcgccctcgc tgcgcccgcg gcaccgaccg ccacggcgcc
     1861 gggccctgcg cccgcatcgc ctgcatcgcc tgcatcgccc gcatcgcctg ctgcgcctgc
     1921 tgcgcctgca tcgcccgcac cgcccgcatc gcctgccccg gcgaccacca ccccggcgcc
     1981 cgcgcccgcg cccgcgcccg cctccgaccc gctgctgacc ccgccgcctg ccgagccggc
     2041 cggcgacgtc ccgcccgacc cggtcacgcc gccatccgag ccggcgcccg cgcccgagcc
     2101 ggtgttgccc ccgatgcgcg acgagccgcc gcccgatcct ccgccgacga tcggcccggg
     2161 cgcggtcgag gcgcccgagc cagcgccgcg gcaccgactg atctacagca acaccctcgt
     2221 cttgcgcgtc aacccgctcg gcctcgagga ccgcttctcg ctgatgtatc gccggcgcct
     2281 gagcgcgcgc accggcaagc tgtgggacga cacctacttc ggcgtcggca tcacgccgag
     2341 cttcgccccc tcgatcaccc gcttcggtcc gacggtgatg ctcgtgcccc tggcgatcct
     2401 ctcgctgcgc gccagctact acttcgtcgg caactacggc agccagagct tcaagccgca
     2461 ccccttcgac tcgcccgccg acgaatacgg ccccgacacg atcaaggccc gcgccgacgc
     2521 caggcagggc atcaacacct acggcggtca ggccgagcta tcggcgctgt tccaggtcaa
     2581 gttcggcccg atcgcgctgc gcaacgaggt gatcttcttt cataacagca tcaagctgcc
     2641 cggcagcaac gacgtctatt atgacatccg ccacgacatc atggcccccg gcaagggctg
     2701 gttcctcagc aacgacaccg acctgctgta caccaacgcc aagctccgcc tcaacgccgg
     2761 cgtgcgcggc acctactatc atatctttta tcccgagacc ctgtacgccc cgggcgacat
     2821 cgccggcacc aaccaaaacg acagcgctcg cgtcggcccg ctgatctcct acgccttcaa
     2881 ggatcgcccc gagaaacgct tcaccaagcc gacccttttc ttcgccgcgc agtggtgggt
     2941 caagcaccgc taccgcaccg gccaggaggt cagccagggc ctccccctga tgatcctcgg
     3001 cttttccttc accggcgagc tgttctcacg gaagtgaacc tgcgccgcgg gagcagcagc
     3061 agcgccagcc acaacgcgcc cggtccggtc ccgccgcgac aaccgcagcc ggcgctgctg
     3121 tcctgcccgc cgtcgtcgct gccctgcccg ccgtcgttgc tcgtatcata cgtcgtgctg
     3181 ccggtgccgc tgccggtccc gtccccgccg tcctcgcccg tcgggctcga gccgccgtcc
     3241 gtgccgccgc tcgtcggcgt cgacccggtc tcactgccgg aagggtcgct cgccgagccg
     3301 gtctgcgcgc ccgtctcgag gcccgtgtcc gtaccggtgt catcaccgcc ctcctcgcag
     3361 ctcgcgggcg gatcgctctc gtcgaccccc tcgggccgca ccccgtagat cggcgtcggg
     3421 cagccgagct cgtgcgcgcc gatgtccggg ccgcccccga gatacagcga attgaccccc
     3481 ggcagcggca agccggcgtc gaccgccggc gagtccgccg cgagcgtgac atccgcctcc
     3541 gccaccgtct cgacataact cgcgggcgcc tgcaacccgc tcgcaaacgt ccctgccgtc
     3601 aacagcaccc cgttcgcctc gaacttcccg gccgcctgca tcgccgcgaa gccctgccac
     3661 ttgtccgcgc cgccgaagtc gaagaagcca tccggataat aaccattgcc gtcgagcacc
     3721 gcgtcgtcga tcggcacgct ccagtcgacg accttgtcgt tctccggcgc ggccggcccg
     3781 acgtacagat tattgatcag gcgaagatca tgggcggtcg ccgccgcctg gagatagacc
     3841 gcgtggcgcg ggctgacgaa ggtgttgtgc atcagcaacg cgccgaccgt ctcgccgagg
     3901 ttcccgttcg agtgcagctt ctgctgctcg tcgacgatgt tgaccgcgac gttgcggatc
     3961 gcatacgccg gtccgccgaa gatcggctgg aagctcagcg gcgaccaaga attcaggaac
     4021 atgttgccga ccgcgcgggc gttgccggcc gtctcgtcga gctcgagcgc gttgtcgtag
     4081 gccgagagcg agaggttgcc gtagacgtcg aacgcacgcg cgccgagctg ctcgatcttg
     4141 atcgagtcgc cgaagccgac caggcggttg tgacagacca cgtgaccgtc gccgctgacg
     4201 acgatgccgt cctcgttggc gaacatccct ccgtcgtcgc cccacaccgc cggccacgcg
     4261 aggcgcccct cgagcacgtt gtcacacaga taaaagttct tctggtccgc cttcgcgccg
     4321 atgccgaggc gaacgtcgcg gatgtgcacg cgccggacca cgttgccctc gctgcccgca
     4381 ccctgaaagc gcagcgcccg ggtcgcggcc tgcagcgtca gccgctcgag gtggacgaag
     4441 ctgccgtaga tctcgagcac gttgcagccg tcacacccgc cgccgtcgac gatcgtgccc
     4501 tccgtgctcg cgccgcggat cacgatcggg tcggccgcgg tgccgctcgc cgagatcgag
     4561 aactgccccg aatatgtccc gtcggcgagg ctgatcacgt cccccggcgc cgctgcggcc
     4621 agcgccgcgc tcaggcccgc cgcgtcgctg accatgaccg tcttcgcctg gcccgggtcg
     4681 gccggcaccg gccgcgtccg gcccatcacc gtccacgtct cgtcgagacc gtccgggtcc
     4741 tgcgcgtgca gctcgatctc gtattcgctg tccgcgcgca gcccgaacac ggtccccgcg
     4801 aactgctgcg gcaccaccag cccgg
//
LOCUS       DASWNJ010002015         3474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_419723, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002015 DASWNJ010000000
VERSION     DASWNJ010002015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419723"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VGB85_33610"
     CDS             <1..1084
                     /locus_tag="VGB85_33610"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HEY0139089.1"
                     /translation="REALLHGPHEQLRARVHAYLREQVAAIVGGPPDFDPDTALADLG
                     VDSLMAVDLHEALRSDLGVDLSLAATLQGDTLEKLGDHLTAALRTATPANLDRTPPAP
                     LTTNTASPWLRAIGPVPATPRLRLICFPHSGAGPTAFLPWRGKLPADVAALAVQLPGR
                     WERLTEHPLTRMDRLLEQLLPALTPLLKSPFAFFGVSMGALVAHALTCALRRAGHPLP
                     AHLFVAAYPAPHLPNPLLRHRDVLREALRDDSGAHPALRRLGLIPEALQGPDTLRLLL
                     PALRADFELVLHHEPREEPPLSVALTALGGREDPEVSREQLAAWLSHTAGDFALRMLP
                     GGHLCFRTHPDATLAAIAPTLHALSH"
     gene            1095..2462
                     /locus_tag="VGB85_33615"
     CDS             1095..2462
                     /locus_tag="VGB85_33615"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0139090.1"
                     /translation="MPSHAPSLPPGPPASRLVQTYRFARHPQSSLDVSFKRYGDAFTA
                     RLFGLGTVVVFSDPAAIEDLFTKPAEELATGECNRRLFEAFLGPHSLLNIDGPRHRRK
                     RRLLMPPFHGQRMSLYGRTMAEITAQEMATWRVGAPFPVQEATQNITLEVILRVIFGV
                     DDPTRRDRLRAAILGFVADSYRPTSAILAFPGAQVDLGPHSPWGRFVRVRDHVYAEIL
                     AAVADRRATGTGGRDDILSLLLDARDEDGQPMSDEELRDDLFTLLVVGHETTAATLAW
                     GMCGVLADPEVTARLREERRQILGDGPLDTARLSELVYLDATVKEITRRYPVTDGAAR
                     LLKVPTQLGRHHLPAGIVATASTWLTHTNPALWRDPLRFAPERFLEARPVPFSLLPFG
                     GGVRTCIGMAFASYEMKIVLSEFLARADLELAPGYTPRVVRRTVTLSPSKGAPVRLRR
                     PVTRA"
     gene            2487..3320
                     /locus_tag="VGB85_33620"
     CDS             2487..3320
                     /locus_tag="VGB85_33620"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0139091.1"
                     /translation="MSTLHADPDAAFEREMSEQYDAFQALNEAWGAGATYFNWGYMTA
                     PGQSYASTQERLVREVFDAAELAPDHVLVDVGFGSGEQDFFAAARYPFARLHGFNVSR
                     RQVEHANRRAAEQGLADRLTFHLEPAEDMRALADGEADRMVAIECAPHFDRPRFYREA
                     ARVLRPGGRLVLADISFAAWAGRLTRNGAPELRRMGDIAGNRAAWDPYFSTHTLRNIN
                     WQTVPGCLRSVWHVLRTVPRVPDRGHRRHWLKLAMTSQIMGTGLLLRLIRYDLIVLER
                     R"
BASE COUNT          444 a         1468 c         1058 g          504 t
ORIGIN      
        1 gcgcgaggcc ctcctgcacg gcccccatga gcagctacgc gcccgcgtgc acgcctatct
       61 gcgcgagcag gtcgccgcga tcgtcggtgg cccgcccgac ttcgatcccg acaccgcgct
      121 cgccgacctc ggcgtcgact ccctcatggc cgtcgacctg cacgaggccc tgcgcagcga
      181 cctcggcgtc gacctctcgc tcgccgcgac cctgcaaggc gacacgctcg agaagctcgg
      241 cgatcacctg accgccgcgc tgcgaaccgc cacgcccgcg aacctcgatc gtaccccacc
      301 ggctccgctg accacgaaca ccgcatcccc atggctgcgc gcgatcggcc ccgtccccgc
      361 caccccgcgc ctgcgcctga tctgcttccc gcacagcggc gccggcccga ccgctttcct
      421 cccgtggcgc ggcaagctgc ccgcggacgt cgccgcgctc gccgtgcagc tgcccggccg
      481 ctgggagcgg ctgaccgagc acccgctgac caggatggac cgcctgctcg agcaacttct
      541 gcccgcgctc accccactct taaaatctcc cttcgccttc ttcggcgtca gcatgggagc
      601 gctcgtcgcc cacgccctga cctgcgcgct gcgtcgcgcc ggacacccgc tgcccgccca
      661 cctcttcgtc gccgcctacc ccgcgccgca tctaccgaac ccgctgctgc gccaccgcga
      721 cgtcctgcgc gaggccctgc gcgacgacag cggcgctcac cctgcgctgc gccggctcgg
      781 cctgatcccc gaggcattgc agggccccga caccctgcgc ctgctgctcc ctgcgctgcg
      841 cgccgacttc gagctcgtgc tgcatcacga gccgcgcgag gagccgccct tgtccgttgc
      901 gctgaccgcc ctcggcggcc gcgaggatcc cgaggtctcg cgcgagcagc tcgccgcctg
      961 gctctcgcac accgccggcg acttcgcgct gcgcatgctg cccggcgggc acctttgctt
     1021 tcgcacccac cccgacgcca ccctcgccgc gatcgccccg accttgcacg ccctgagcca
     1081 ctgacatgcc tgacatgcct tctcatgccc cctccctgcc ccctggcccg ccggcctcgc
     1141 gcctggttca gacctatcgc ttcgcccgcc acccgcagtc ctcgctcgac gtctcgttca
     1201 agcgctacgg cgacgccttc accgcgcgcc tgttcggcct cggcaccgtc gtcgtgttca
     1261 gcgacccggc cgcgatcgaa gatctattca ccaagcccgc cgaggagctg gcgaccggcg
     1321 agtgcaaccg gcggctgttc gaggccttcc tcggcccgca ctcgctgctc aacatcgacg
     1381 gcccgcgtca ccgtcgcaag cgccgcctgc tgatgccgcc cttccacggc cagcgcatgt
     1441 cgctgtacgg ccgcacgatg gccgagatca ccgcgcagga gatggcgacc tggagggtcg
     1501 gcgccccgtt cccggtccag gaggcgacgc agaacatcac cctcgaggtc attctgcgcg
     1561 tcatcttcgg cgtcgacgac ccgacccggc gcgaccgcct gcgcgccgcg atcctcggct
     1621 tcgtcgccga ctcctaccgg ccgacctcgg cgatcctcgc gttccccggc gcgcaggtcg
     1681 acctcggccc gcactcgccg tggggtcgct tcgtccgcgt gcgcgatcac gtctacgccg
     1741 agatcctcgc cgccgtcgcc gatcgccggg ccaccggcac cggcggccgc gacgacatcc
     1801 tgtcgctgct gctcgacgcc cgcgacgagg acggccagcc gatgagcgac gaggagctgc
     1861 gcgacgacct gttcacgctg ctcgtcgtcg gccacgagac caccgccgcg accctcgcgt
     1921 ggggcatgtg cggcgtgctc gcggacccgg aggtcaccgc caggctccgc gaggagcggc
     1981 gccagatcct cggcgacggt ccgctcgaca ccgcacgcct gtccgagctc gtctacctcg
     2041 acgccaccgt caaggagatc acgcgccgct accccgtcac cgacggcgcc gcgcgcctat
     2101 taaaagtgcc gacgcagctc ggccggcatc acttgcccgc gggcatcgtc gccaccgcct
     2161 cgacctggct gacgcacacc aacccggccc tgtggcgcga cccgctgcgc ttcgcccccg
     2221 agcgcttcct cgaggcgcgc ccggtcccct tcagcctgct gccgttcggg ggcggcgtgc
     2281 gtacctgcat cggcatggcc ttcgccagct acgagatgaa gatcgtgctg tccgaattcc
     2341 tcgcgcgcgc cgacctcgag ctggcccccg gctacacccc cagggtcgtc cgtcgcaccg
     2401 tcaccctctc gccgagcaag ggcgcgccgg tccgcctgcg ccgccccgtg acccgcgcat
     2461 aaccccgtcc ccgcgagccc cacgatatgt ccacactcca cgcagacccg gacgccgcct
     2521 tcgagcgcga gatgtccgag caatatgatg ccttccaggc gctcaacgag gcctggggcg
     2581 ccggcgcgac ctacttcaat tgggggtaca tgacggcccc cggccagtcg tatgcgagca
     2641 cccaggagcg gctcgtgcgc gaggtcttcg atgccgccga gctcgcgccg gaccacgtgc
     2701 tcgtcgacgt cggcttcggc agcggcgagc aggacttctt cgccgccgcc cgctacccct
     2761 tcgcgcgcct gcacggcttc aacgtctcgc gccggcaggt cgagcacgcc aaccgccgcg
     2821 ccgccgagca gggcctcgcc gaccgcctga ccttccacct cgagccggcc gaggacatgc
     2881 gcgcgctcgc cgacggcgag gcggaccgca tggtcgcgat cgagtgcgcc ccgcacttcg
     2941 accgcccgcg cttttatcgc gaggccgcgc gggtgctgcg acccggcggt cgcctcgtgc
     3001 tcgccgacat cagcttcgcc gcgtgggccg gccgcctgac ccgcaacggc gccccggagc
     3061 tgcgccgcat gggtgacatc gcgggcaacc gcgccgcgtg ggacccgtat ttttcgacgc
     3121 acaccctgcg caacatcaac tggcagaccg tgcccggctg cctgcgctcg gtgtggcacg
     3181 tgctgcgcac cgtgccgcgg gtgcccgatc gcggccatcg ccggcactgg ctgaagctcg
     3241 ccatgacctc gcagatcatg ggcaccggcc tgctcctgcg cctgatccgc tacgacctga
     3301 tcgtgctgga gcgccggtga ccggcgccgc ccccggagcc ccgccgtccc cgaagggtct
     3361 gccgatcctc ggcaacctcg tcgagtggtt gcgcgacccc ttcgtcgcgc cgacccgctg
     3421 ggcccgcgag cacggcgaca tcatccgcct gcgcgcgccc ggccccgcga ccta
//
LOCUS       DASWNJ010002016         1570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_419793, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002016 DASWNJ010000000
VERSION     DASWNJ010002016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419793"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VGB85_33625"
     CDS             <1..507
                     /locus_tag="VGB85_33625"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0139092.1"
                     /translation="AIPFGLVGAIGGHVMLGLELSIMSMMGMVALSGVVVNDSLVMVD
                     ATNEFHQRQGMSHFEAVAAAGARRFRPIMLTSITTFFGLAPMILEPSVQAKFLIPMAV
                     SLGFGVMFATAITLLIVPALYLAVEDGKNMWRWLMERPIESTEPDPPGTQAQRRTIGP
                     MDPIDVEV"
     gene            504..>1570
                     /locus_tag="VGB85_33630"
     CDS             504..>1570
                     /locus_tag="VGB85_33630"
                     /inference="COORDINATES: protein motif:HMM:NF036723.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0139093.1"
                     /translation="MNVFYQDAPGNPDLFRTDLALHSELARRLPEDLRAAAQGRCAAL
                     GVATTEELPRLAMQAEAQPPQHVPYDAWGRRVDEIRTSPAWESLRVFSARNGIVATGY
                     DLALGEHRRVVQAALLHLFSASSATYSCPLAMTDAAARVLLDLAPASLRDRLVSGLTS
                     TDPARFITSGQWMTERTGGSDVGNTETIARPAGSSADGRQYTLHGVKWFTSATTSEMA
                     LTLARIDDGGAPPVPGSRGLSLFCVEISRISGQGWRGITVHRLKDKLGTKALPTAELT
                     LDGAPATLIGEPGRGVANISGMLNVTRLYNAIASASGMHRACALARDYAERRSAFGRK
                     LVDLPLHAATLADMESETAAAL"
BASE COUNT          220 a          567 c          547 g          236 t
ORIGIN      
        1 gcgatcccct tcgggctcgt cggcgcgatc ggtggccacg tgatgctcgg cctcgagctg
       61 agcatcatga gcatgatggg catggtggcg ctctcgggcg tggtggtgaa cgactcgctg
      121 gtgatggtcg acgcgaccaa cgagtttcac cagcgccagg gcatgtcgca cttcgaggcg
      181 gtcgcggccg ccggcgcgcg ccggttccgg ccgatcatgc tgacctcgat cacgaccttc
      241 tttggcctcg cgccgatgat cctcgagccc tcggtgcagg ccaagttcct gatcccgatg
      301 gccgtcagcc tcggcttcgg cgtgatgttc gcgaccgcga tcaccctgct gatcgtcccg
      361 gcgctgtacc tcgcggtcga ggacggcaag aacatgtggc gctggctgat ggagcgaccg
      421 atcgagtcga ccgagcccga cccgccaggc acgcaggcac agcggcgcac tatcggcccg
      481 atggacccga tcgacgtgga ggtatgaacg tgttttatca ggacgccccc ggcaaccccg
      541 acctgtttcg caccgacctc gcgctgcaca gcgagctggc ccgccgcctg cccgaggacc
      601 tgcgcgccgc ggcgcagggg cgctgcgccg cgctcggcgt ggcgaccacc gaggagctgc
      661 cgaggctggc gatgcaggcc gaggcccagc cgccgcagca cgttccctat gacgcatggg
      721 gccggcgcgt cgacgagatc cgcacctcgc ccgcgtggga gtcgctgcgc gtgttctcgg
      781 cgcgcaacgg gatcgtcgcc accggttatg atctcgccct gggcgagcac cgccgcgtgg
      841 tccaggcggc gctgctgcac ctgttctccg ccagctccgc gacctacagc tgtccgctgg
      901 cgatgaccga cgcggccgcc cgcgtgctgc tcgacctcgc gcccgcgagc ctgcgcgacc
      961 gcctggtgtc cggcctgacg agcaccgacc cggcgcgctt catcacgtcc ggccagtgga
     1021 tgaccgaaag gacaggtggc tcggacgtcg gcaacaccga gacgatcgcg cggccggccg
     1081 ggagcagcgc cgatggccgg cagtacaccc tgcacggcgt gaagtggttc acctcggcga
     1141 cgacctccga gatggcgctg acgctcgcgc gcatcgacga cggcggcgcg ccgcctgtcc
     1201 ccggctcgcg cggcctgtcg ctgttctgcg tcgagatctc gcggatctcc gggcagggct
     1261 ggcgcgggat cacggtgcat cgcctcaagg acaagctcgg cacgaaggcg ctgccgaccg
     1321 ccgagctgac cctcgacggc gcacccgcga cgttgatcgg cgagcccggc cgcggcgtcg
     1381 ccaacatctc gggcatgctc aacgtgacgc ggctttataa tgcgatcgcg tcggcgtcgg
     1441 gtatgcaccg cgcctgcgcg ctggcgcggg actatgccga gcggcgcagc gcgttcgggc
     1501 gcaagctcgt cgacctgccg ctgcacgccg cgacgctcgc cgacatggag tcggagacgg
     1561 ccgccgcgct
//
LOCUS       DASWNJ010002017         5786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_419992, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002017 DASWNJ010000000
VERSION     DASWNJ010002017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 5786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5786
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419992"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(259..741)
                     /locus_tag="VGB85_33635"
     CDS             complement(259..741)
                     /locus_tag="VGB85_33635"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY0139094.1"
                     /translation="MHAPLRHVPRPRRRCRVPTRDHDDRRAVDLKAIWAEDVAAVERL
                     LRDGADVHAAGATGRTPLMQAAEAENLRIIDRLLEAGADVNATGDAGQTALHCAVDMA
                     IDGAIQNDREVSAQSIVTIERLLRAGADVHARDDRGQSPADWARRDSLVLAFIQRARP
                     "
     gene            1053..1532
                     /locus_tag="VGB85_33640"
     CDS             1053..1532
                     /locus_tag="VGB85_33640"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY0139095.1"
                     /translation="MVARDPSQAQRQRLASPLARLAACFLDGITAAASVAVFGYLGSE
                     LAGSLAWCAAYLYLMVEYGQTPGKALMGIQIVRGDGRPVGFLRGAIVRTLAFPLLLAA
                     IVALVARLSPNMLASLGEDQLHGVLAGLIVISYLTIFGRNRRTLNDYLAGTHVVREA"
     gene            1764..2273
                     /locus_tag="VGB85_33645"
     CDS             1764..2273
                     /locus_tag="VGB85_33645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139096.1"
                     /translation="MRVSPPLAYTLLLCVAACGSESSEPPEEHTEEIAAGCGFPRPCG
                     ELTIDCGTSQDPAVCPEDYSPETRCALEQLAADGQFAARIDTTEYNMTDEQHFDVVFD
                     GRGFAVRQRWDDGVPVGPPEKCILRMKEYYSLCLSAANSDPLHSSCKVALDWFADCEH
                     VAGLECPTP"
     gene            complement(2419..3516)
                     /locus_tag="VGB85_33650"
     CDS             complement(2419..3516)
                     /locus_tag="VGB85_33650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY0139097.1"
                     /translation="MHSRLDLLVPLAPLFASLLAGCAQDSCSDFKGGTYMQTIELTPA
                     DYEQWKMGMQPGEAPTSSSTMGAASDSSGTAGGSTGGGDDTGSGDTTTGDAPTTSEPV
                     MLTDQEICMMVCSDATAGGEVESCTIGAMNASGNIPVECVLQSFCTGRRHACVRSQGG
                     VAGSEPAADWRARAAHDAAASVHAFLALAAELAAHGAPAGLLARIDAAVDDERRHAAL
                     ITDLAHRHGAEVPAVSIAPTPPRDLLALAVENVVEGCVRETWAALSDAHQARHAHTAE
                     LRDLYASIAADEASHAELAWAIDAWLFGQLGEDGRNMVAAARRQAVRQLQASLATLGD
                     DPGLRALGVPRRDDALHLAAGLDAALWSQAA"
     gene            3844..5334
                     /locus_tag="VGB85_33655"
     CDS             3844..5334
                     /locus_tag="VGB85_33655"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0139098.1"
                     /translation="MTDARETGTHDPSPRPLAGLLRELFDADELRIHLASEPDGASLV
                     GALPGAAVSLELLTFIAAEALKRRGLVSRDFFDRLEVARPHRRDAIRKARNAWFAGVH
                     PEAGALWGDRYRLDVQLGDGGFGSVWRAVDEQSGQFVALKVLHRAHIDNPRIRKRFFR
                     GAHTLAGLSHPSIVRVHAAALEAGLHSYYVMEYIRGATLDTCVRERRYPRSELIEMAL
                     QIGDALIHVHQRGLLHRDVKPANILITDRKLARLIDFDLVTGDDFVALTTEAVGTQLY
                     MPPEAHTSDSKTGAYDVFSLARTIELILRGREPTVRELADVADIDASADVRAILRAAL
                     QPDPQRRIAQMATFCAVLRAALAAEGSAPPRPRRSWPSASREPSSATRPYATWCAAAS
                     IVGHLAVTTYLAPPREHALFLFIFFTGPSIRVFLDRERNRYLTILHVIWMGLWSLPVL
                     VLLDLLLGNTRSSSSRVLPALVVLGAIALGAYASVVHLRGPRRRGG"
     gene            complement(5462..5677)
                     /locus_tag="VGB85_33660"
     CDS             complement(5462..5677)
                     /locus_tag="VGB85_33660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139099.1"
                     /translation="MRIVLHNCNLADAERVLILVALAEMGDLEGAAELCGVTWARLRH
                     LLRVHGIEWPGVGGRRRRQRRAPRGSD"
BASE COUNT          849 a         2021 c         1998 g          918 t
ORIGIN      
        1 tgtcgacctt gcgcgcgacc ggttagcggt cgggtgccgg actttggatt aaccgccggc
       61 gcgtgacaag catccacgag cggtagtttg cgcccgtgat ccgccgggcc gccggtcacc
      121 ccatgcgcgc gaaggctccg cgcggcaggg ggtccgatgc cgcgacgatc cctggacgca
      181 gatgatgcgc gccaggtcga cgacaacgtc ggccggtcgg tcgcaggcgg atctccgtgg
      241 ctgccgagcc gagccggttc acgggcgtgc gcgctggatg aaggcgagca cgagcgaatc
      301 tcggcgcgcc cagtcggccg gcgactgccc gcggtcgtca cgggcgtgca cgtcggcgcc
      361 ggctcgcagc agcctctcga tcgtgacgat cgactgcgca ctgacttcac ggtcgttctg
      421 gatcgcgccg tcgatcgcca tgtcgacggc gcagtgcagc gcggtctggc cggcgtcacc
      481 ggtcgcgttg acgtctgcgc cagcctcgag caatcgatca atgatccgta gattctccgc
      541 ctccgccgcc tgcatgagcg gcgtccggcc ggtggcgccc gccgcatgga cgtcggcgcc
      601 gtcgcggagc aagcgctcga ccgccgcgac gtcctccgcc cagatggcct tgagatcaac
      661 cgctcgtcgg tcatcgtgat ccctcgtcgg cacacggcac cgtctgcgcg gtcgggggac
      721 gtgtcgcaaa ggggcgtgca tatcgtcgta agggtgataa cgacagcgcg acgatcccgc
      781 ggatcgatcg atggggcggc ggcgtgaagt cgtcgccgcg gggagcgagc gccggcggag
      841 ccacggacgt gtgagcgcgc gagacgaccg tcatggcgat cactatcgca catcgccacc
      901 ggacagcctg gtccgctcga cgtccggcgg atccgcctgc atgagcacgc cagcggcaaa
      961 attcacgcgg ctgtgatcca attcctcggc tggcggcact gacagagtcg atggctcacg
     1021 ctcccacgcc ccgtgcccgg tccctctcgc cggtggtcgc ccgcgacccg tctcaggcgc
     1081 agcggcagag gctcgcgtca ccgctcgcca ggctcgccgc gtgcttcctc gatggcatca
     1141 cggcggcggc gtccgtggcc gtgttcggct acctcggctc ggagctcgcc gggagcctgg
     1201 cgtggtgcgc cgcgtacctc tatctcatgg tcgagtacgg gcagacgccc ggcaaggcct
     1261 tgatggggat ccagatcgtt cgtggcgacg ggcgtcctgt cggcttcctt cgcggggcga
     1321 tcgtccgcac gctcgccttc ccgctgctgc tcgccgcgat cgtcgccctc gtggcccggc
     1381 tctccccgaa catgctcgcc agcctcggcg aggaccagct tcatggggtg ctcgccgggc
     1441 tcatcgtgat ctcgtatctt acgatcttcg ggcgtaatcg tcgcaccttg aacgactatc
     1501 tcgctggcac gcacgtcgtc cgtgaagcct gatacggcga gtcacatcag ttttaaaaag
     1561 gaccgcgccg ccgtatgctc gggtggggac gcaactgcgc tgaaccgcgc ttgccgtctc
     1621 gtccgagctc ctccgccggg caaggtcgag gcactgatcg cggcacggac ggccgatccg
     1681 gcgcgtcggc ctgaattggc gcgaagctcc gggacaaagc cccggagacg acagggcaga
     1741 tgacacaggc tatcctcgcc gctatgcgcg tctcgccgcc tctcgcttac acccttttat
     1801 tgtgcgtcgc cgcctgcggg tccgagagca gcgagccgcc cgaggaacat accgaggaga
     1861 tcgccgcggg ctgcggcttc ccgcggccct gcggggagtt gacgatcgac tgcggtacct
     1921 cccaggaccc cgcggtctgc ccggaggact actcgcccga gacccggtgc gcgctcgagc
     1981 agctggccgc cgacgggcaa ttcgccgctc gcatcgacac caccgaatac aatatgacgg
     2041 acgagcagca cttcgacgtg gtcttcgacg gcaggggttt cgccgtgcga cagcgctggg
     2101 acgacggtgt gcccgtgggg ccgcccgaga agtgcatctt gcgcatgaag gagtactaca
     2161 gcctgtgtct cagcgcggcg aacagcgacc cgctgcactc ctcctgcaag gtcgccctcg
     2221 actggttcgc ggactgcgag cacgtcgcag gcctggagtg cccgacgccc tgaggcagga
     2281 gcgccggctc cgtgcgaggc accgcgctga aatactgaca ttgtaccagg cgccgcgcga
     2341 cgcccaatga caggggtttg ccgcgctggc cgagggtccg tcgaggaggc acggacgggc
     2401 cggcgagtga tgcccgcctc aggcggcttg cgaccacagc gcggcgtcga ggccggcggc
     2461 caggtgcagc gcgtcgtcgc ggcgcggcac gccgagggcg cgcaacccgg gatcgtcgcc
     2521 gagcgtggcg aggctggcct ggagctggcg cacggcctgg cgccgggccg ccgcgaccat
     2581 gttccggccg tcctcgccga gctgtccgaa cagccaggcg tcgatcgccc aggcgagctc
     2641 ggcgtggctg gcctcgtcgg cggcgatgct cgcgtagagg tcgcgcagct cggcggtgtg
     2701 ggcgtgccgg gcctgatgcg cgtccgagag cgcggcccag gtctcgcgca cgcagccctc
     2761 gaccacgttc tcgacggcga gcgcgagcag gtcgcgaggc ggcgtcgggg cgatcgagac
     2821 cgccgggacc tcggcgccgt gacggtgcgc gaggtcggtg atcagcgcgg cgtggcgacg
     2881 ctcgtcgtcg acggcggcgt cgatgcgcgc gagcagcccg gccggagcgc catgggccgc
     2941 gagctcggcg gcgagggcga ggaaggcgtg cacgctggcc gccgcgtcat gggccgcgcg
     3001 ggcccgccag tcggccgcgg gctcgctgcc cgcgacaccg ccctgcgagc gcacgcaggc
     3061 gtgacggcga cccgtgcaga acgactgcag cacgcactcg acggggatgt tgccgctggc
     3121 gttcatcgcc ccgatcgtgc aggattcgac ctcgccgccg gcggtcgcgt cgctgcacac
     3181 catcatgcag atctcctgat cggtcagcat cacgggctcg ctggtggtcg gtgcatcgcc
     3241 ggtcgtggta tcgccggacc ccgtgtcgtc gccgccgccc gtggagccgc ccgcggtgcc
     3301 gctggagtcg ctcgcggcgc ccatggtcga gctgctcgtg ggcgcctcgc ccggctgcat
     3361 gcccatcttc cactgctcgt aatccgccgg ggtcagctcg atggtctgca tgtacgtgcc
     3421 gcccttgaag tcgctgcagc tgtcctgggc gcagccggcg agcaacgagg cgaacagagg
     3481 cgcgagcggg acgagcaagt cgaggcgaga atgcatacga tacagctacg cacaaggtcg
     3541 cgataaagtc gacccgccgt gctgcatcgc gcgggagcgc ccgtgtccgc gtcactcgtc
     3601 gagacgctgg cagaccggcg aggcgtcgta gttcatcaag acttgcagct ccgccgacat
     3661 ccatcgccgc gtgtcgacgg tcgatcgcgg cgccgatcaa gggtcgacgg gcgtgacgaa
     3721 gcgccggacc cctgtacctg aaggtgggat acagagcatc actgctcgac agcgcaggta
     3781 ttggcgggct cgacgggtac gatccacgcc catgtgcacg ccggctgacg tacgatgccg
     3841 ccgatgaccg atgcacggga gaccggcacg catgacccct cgcccaggcc gctcgccggc
     3901 ctcctgcgcg agttgttcga cgcggacgag ttacgcatcc acctcgccag cgagccggac
     3961 ggtgccagcc tcgtgggggc gctgcccggc gctgcggtgt ccctcgagct gctcacgttc
     4021 atcgctgccg aggccctcaa gcggcgcggc ctggtgagcc gtgacttctt cgaccgcctc
     4081 gaggttgcgc gaccgcatcg acgcgacgcg atccgaaagg cccgcaatgc atggttcgca
     4141 ggggtgcatc ctgaggccgg cgcgctgtgg ggcgaccgct accgtttgga cgtacaactc
     4201 ggcgacggcg gcttcggcag cgtctggcgg gcggtcgacg agcagagcgg gcagttcgtc
     4261 gcgctcaagg tccttcaccg ggcacacatc gacaaccccc ggatccgcaa gcgcttcttt
     4321 cgcggcgctc acacgctcgc ggggctgtcc catccctcga tcgtccgcgt tcacgccgcc
     4381 gcgctggaag ccggcctgca cagctactat gtcatggagt atatccgcgg tgcgaccctc
     4441 gacacatgcg tgcgcgagcg ccggtacccg aggtccgagc tgatcgagat ggcgctgcag
     4501 atcggtgatg cgctgatcca tgtccaccag cgaggccttc tccaccggga cgtcaagccc
     4561 gccaatatcc tgatcaccga ccgcaagctc gcccggctga tcgacttcga cctggtcact
     4621 ggcgacgact tcgtcgcgct caccaccgag gccgtcggaa cccaactcta catgccaccc
     4681 gaggcgcaca cctcggactc caagaccgga gcgtacgacg tattcagtct tgcgcgcacg
     4741 atcgagctca tcctccgcgg ccgcgagccg actgtccgcg agctggccga cgtcgccgac
     4801 atcgacgcgt cggccgacgt cagggcgatc cttcgggctg ccctccaacc ggacccgcaa
     4861 cgccgcatcg cccagatggc gaccttctgc gcggtcctcc gcgcggcgct ggcagccgag
     4921 ggatccgcac cgccccggcc gaggcgaagc tggccatccg cgtcccggga gccttcgagc
     4981 gcgacgcgcc cgtatgcgac atggtgcgcc gccgcgtcga tcgtcgggca tctcgcggtc
     5041 accacatatc tggctccgcc gcgcgagcac gccctgttcc tgttcatctt cttcacaggg
     5101 ccgagtatcc gggtgttcct cgaccgcgag cgcaaccggt atttgacgat ccttcatgtc
     5161 atctggatgg gcctgtggtc gctcccggtg ctggtgctcc tggatctgtt acttggcaat
     5221 acccgatcca gctcgagcag agtgctccct gccctcgtcg tgctcggggc gatcgcgctc
     5281 ggggcttatg ccagcgtcgt ccaccttcgt ggcccgcgtc gtcgcggggg ctgagaggcg
     5341 ctttcaaggc ggccggatcg gtgatggaga tcggtcggtc cgcgaacgtc attccaggcg
     5401 gtggtgccaa accgcgtccg aaccggtggt agcagcgccg gggaccgggc gcgtggggac
     5461 ctcagtcaga cccgcggggc gctcgccgct ggcgcctgcg ccttccgccg acgcccggcc
     5521 actcgattcc gtggacccgc agcaggtgcc gaagccgggc ccaggtgacg ccgcatagct
     5581 cggcggcgcc ctcgaggtcg cccatctcgg cgagcgccac gaggatcaag acgcgctcgg
     5641 cgtcggccag gttgcaattg tgaaggacga ttcgcatgct tgcctttcgt ggcaggagcg
     5701 ccgatgcgag gagaccaatg gcgctacggc ggtggtatcc acggcatatt cgcgggcggt
     5761 gtcgctgaaa ctatgtagat atcgca
//
LOCUS       DASWNJ010002018         1993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_632958, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002018 DASWNJ010000000
VERSION     DASWNJ010002018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632958"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..774
                     /locus_tag="VGB85_33665"
     CDS             97..774
                     /locus_tag="VGB85_33665"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY0139100.1"
                     /translation="MDLRQALIFVLCLVVATAFHEFAHAIVAHKLGDPTPSRDGRVTL
                     NPLVHADPIGTVALPIMAGLLHAPLFGWGRPVMTQPRFYTRKFSMRAGMAMVSFAGPA
                     SNLLQAIVTLVVLKVLLVTSVIDPDTFARSRGYALDPTSMIGILVSFIQLNLVLALFN
                     LLPIHPLDGGKILAWMLPARFSHIDDTLARYGWTILLALMLIPGLLLVLMTPVDIAMR
                     WAITLLG"
     gene            784..>1993
                     /locus_tag="VGB85_33670"
     CDS             784..>1993
                     /locus_tag="VGB85_33670"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEY0139101.1"
                     /translation="MAQGSVEAAEARAAIYSVELDLFEGPLDLLLHLVRRHELDILDI
                     PIAFVAEKYLEYLHLMRSLDLEIAGDYLVMAATLAWLKSRELLPQGPREGEQAGADDE
                     EGEDPREALIRRLVEYERFKSAAAELDALPISGRDVFARGADIDVPPLDPGLAPVTLF
                     RLAEAYYRVLTRAKINKTHDVTIELVTVAQRMQQLTLMLDERQEFDFEGLFLGRTWNS
                     EHELRMMVVVTLMSVLELVKLGVARVQQVPDTDAIRVYRLVASEDARKILESYDEEQS
                     FGAPADAAAGPPQPTEAELDALEERTLLAEALAGLDGTEEPPEPADEIGVLAEVLDPE
                     ALAEDDDFAMIPDEADLGPEAIAAPAANNAGVPAPAVDDGDGAQPDTPPDLADMSEAP
                     AVPSDTSEVYG"
BASE COUNT          288 a          750 c          652 g          303 t
ORIGIN      
        1 ccccccagcc ccgtggatcg cgcgttcaca gccctttccg ccgcctcggc gtcgttcgtc
       61 ccggtctcgg cggtttcgct gctgggcatc agcgcgatgg acctgcgtca ggcgctgatc
      121 ttcgtgctct gcctcgtggt cgccacagcc tttcacgagt tcgcccacgc gatcgtcgcc
      181 cacaagctcg gcgatccgac gccgagccgt gacggtcgcg tcacgctcaa cccgctggtc
      241 cacgccgacc cgatcggcac cgtcgcgctg ccgatcatgg ccggcctgct gcacgcgccg
      301 ctgttcggct ggggccgccc ggtgatgacc cagccgcggt tttacacgcg caagttcagc
      361 atgcgcgccg gcatggcgat ggtctccttc gccggtccgg ccagcaacct gctgcaggcg
      421 atcgtcacgc tcgtggtgct caaggtccta ctggtcacca gcgtgatcga cccggacacc
      481 tttgcgcgct cccggggcta cgcgctcgac ccgacgagca tgatcggcat cctcgtcagc
      541 ttcatccagc tcaacctggt cctcgcgctg ttcaatctgc tcccgatcca cccgctcgac
      601 ggcggcaaga tcctggcgtg gatgctgccg gcccgcttca gccacatcga cgacacgctc
      661 gcgcgctacg gctggacgat cctcctcgcg ctgatgctca tcccgggcct cctgctcgtc
      721 ctgatgacgc cggtcgacat cgccatgcgc tgggcgatca cactgctcgg ctgaggagag
      781 acggtggcgc agggatccgt ggaagccgca gaggcgaggg cggcgatcta ttcggtcgag
      841 ctcgacctct tcgagggtcc gctcgacctc ctgctgcacc tcgtgcgccg ccacgagctc
      901 gacatcctcg acatcccgat cgccttcgtc gcggagaagt acctcgagta cctccacctg
      961 atgcgctcgc tcgacctcga gatcgccggc gactacctcg tgatggcggc gacgctcgcg
     1021 tggctcaaga gccgcgagct gctgccgcag ggcccgcgcg agggtgagca ggccggcgcc
     1081 gacgacgagg agggcgagga cccgcgcgag gcgctgatcc gccggctcgt cgagtacgag
     1141 cggttcaaga gcgccgccgc cgagctcgac gcgctgccga tctccgggcg cgacgtgttc
     1201 gcccgcggcg ccgacatcga cgtcccgccg ctcgaccccg ggctcgcgcc ggtgaccctg
     1261 ttccgcctcg ccgaggcgta ctaccgggtc ctgacgcggg ccaagatcaa caagacccac
     1321 gacgtcacga tcgagctcgt caccgtcgcc cagcgcatgc agcagctgac gctgatgctc
     1381 gacgagcgcc aggagttcga cttcgagggc ctgttcctcg gccgcacctg gaacagcgag
     1441 cacgagctgc gcatgatggt cgtcgtcacg ttgatgtcgg tgctcgagct cgtcaagctc
     1501 ggcgtcgctc gcgtgcagca ggtgcccgac accgacgcga tccgcgtgta tcgcctggtc
     1561 gccagcgagg acgcgcgcaa gatcctcgag agctacgacg aggagcagag cttcggcgcc
     1621 cccgcggacg cggcggccgg gcccccgcag ccgaccgagg ccgagctcga cgcgctcgag
     1681 gagcgcacgc tgctcgccga ggcgctcgcc ggcctcgacg gcaccgagga gccgccggag
     1741 cccgccgacg agatcggcgt gctcgccgag gtcctcgacc cggaggcgct cgccgaagac
     1801 gacgacttcg ccatgatccc cgacgaggcc gacctcggcc ccgaggcgat cgccgccccc
     1861 gcggccaaca acgccggcgt gcctgcgccg gcggtggacg acggggacgg cgcgcagccc
     1921 gatacgccgc ctgaccttgc ggacatgtcc gaagcgccag ccgtgccgtc ggacacctcg
     1981 gaggtgtacg gcg
//
LOCUS       DASWNJ010002019         3881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_348606, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002019 DASWNJ010000000
VERSION     DASWNJ010002019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348606"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1503)
                     /locus_tag="VGB85_33675"
     CDS             complement(166..1503)
                     /locus_tag="VGB85_33675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY0139102.1"
                     /translation="MSEVSAELRRLSVLATPIVATQLGTMMMGVVDMVMVGGLGSDAL
                     AAAALGGVWTMGTLIIGMGVTLGMDPLLTQAHGAGDGRAVGLTLQRGLVMALLASVPI
                     MVLWFWTGPALVLAGQSPVLSGMAHDYVIVQLPSAIGFLGFTVLRSYLQGRSIVAPAL
                     WITLLANIVNAGLNWLLIFGELGFPALGLTGAGIATGVTRVLMFVALALLIWRTGLHH
                     GAWQPWSRAAFAPAGLRAIALLGVPVGLQLGLEMWAFQAATLFAGWLGEVELAAHTIV
                     LNLASLAFMVPLGISMAAVTRVGNLLGAGDRGGAQRASWIALGMGAGVMCVSSVSFIA
                     LRWVLPRAYTADPAVVVAAASLLPIAAAFQLFDGMQVVGGGILRGMGRTRPAAWFNLL
                     GYYVLGLPLALYLAFETAMGVAGIWWGLTLGLFVVAASLVAWVGARGPAHARV"
     gene            1621..2202
                     /locus_tag="VGB85_33680"
     CDS             1621..2202
                     /locus_tag="VGB85_33680"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY0139103.1"
                     /translation="MSPLHVLRTPRLHLRPLTPDDIPVIARYRDDPEVARHQSWDRYT
                     EDDARALVEQLAGGTPGTPGQWFQWGITRASDGLLLGDCGLKIVGEEPRLGQIGYTLG
                     RAHQRQGYATEAVRAVLDLAFHRLHLHRVSASVDADNLASLALLERLGLRREAHFRKS
                     EWFKGAWVDDVIYAILATEWPAAPDSERSPRSA"
     gene            2218..2358
                     /locus_tag="VGB85_33685"
     CDS             2218..2358
                     /locus_tag="VGB85_33685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139104.1"
                     /translation="MSHRASVASPGRPAFAGQLTVVPLLPGTKVQVSADAPTDPVVVT
                     VP"
     gene            complement(2380..2697)
                     /locus_tag="VGB85_33690"
     CDS             complement(2380..2697)
                     /locus_tag="VGB85_33690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139105.1"
                     /translation="MRTFIKKICSFGFIGLGLLGSMTACHPKATPTAALKSENLGSEM
                     PESLRCKQSSDCVVQTTCYWSEATCVAASSMVAPKCEDADPQQASRADVTCGCSAGQC
                     VVN"
     gene            3000..>3881
                     /locus_tag="VGB85_33695"
     CDS             3000..>3881
                     /locus_tag="VGB85_33695"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEY0139106.1"
                     /translation="MTAAKVTATHIEVLLPIRPATLDTPVFDPDNTVLDPPSPQKRDY
                     VWSTFLPFVGVHLLCFGALWTGARPVDWVVCGVLYFVRVFGVMAGYHRYFSHRTFKTS
                     RWFQFVLALLAESSLQKGVLWWAAHHRVHHKHTDEPADRHSPLQYGFWHSHVLWLAER
                     GSEETVRARVRDLEKFPELVWLDKNWMVPPIAMGLATWLAFGWSGLFVGFFLSTVLAW
                     HATFVVNSLAHVFGTRRFATRDTSRNNWFIALFTLGEGWHNNHHHYMGAARQGFYWWE
                     IDITYYILLGLERCGIVH"
BASE COUNT          624 a         1478 c         1219 g          560 t
ORIGIN      
        1 tggaccagct cacgatagaa gtcgagcgcc ccgctaaact gcgtgaccag gttctcgacc
       61 gcctggaccg tgaagtcgac ctcgtgctcg ctgggcgccg tcatcgacct tgcatcgtag
      121 accctgcgtc acgcatccac cagagtggga tcggcgccgc tgtgctcata cacgcgcgtg
      181 ggcaggcccg cgtgcgccga cccaggcgac cagcgacgcg gcgaccacga acagcccgag
      241 cgtcagcccc caccagatcc cggcgacgcc catcgctgtc tcgaaggcca ggtacagcgc
      301 gagcggcagc ccgagcacgt agtagccgag caggttgaac cacgccgccg ggcgcgtgcg
      361 ccccatgccg cgcaggatcc ccccgccgac cacctgcatg ccgtcgaaca gctggaacgc
      421 cgccgcgatc ggcagcaagc tcgccgctgc gacgaccacc gccggatcgg ccgtgtaagc
      481 ccgcggcagc acccagcgca gcgcgatgaa gctgaccgac gacacgcaca tcacccccgc
      541 gcccatcccg agcgcgatcc acgaggcccg ctgcgcgcca ccccggtcgc ctgccccgag
      601 caggttgccg acgcgggtca ccgccgccat cgagatcccc agcggcacca tgaacgccag
      661 cgacgccagg ttgaggacga tcgtgtgcgc cgcgagctcg acctcgccga gccacccggc
      721 gaacagcgtc gccgcctgga acgcccacat ctcgagcccg agctgcaggc cgaccgggac
      781 gccgagcagg gcgatcgcgc gcaggcccgc gggcgcgaac gcggcgcggc tccacggctg
      841 ccacgcgccg tgatgcagcc cggtgcgcca gatcagcagc gccagcgcga cgaacatcag
      901 cacgcgcgtc accccggtcg cgatcccggc gccggtcagg ccgagcgccg ggaacccgag
      961 ctcgccgaag atcagcagcc agttgagccc ggcgttgacg atgttcgcca gcagcgtgat
     1021 ccacagcgcc ggcgcgacga tgctgcgccc ctgcaagtac gaccgcagca ccgtgaaccc
     1081 gaggaagccg atcgcgctcg gcagctgcac gatcacgtag tcgtgggcca tcccggagag
     1141 caccggcgac tgcccggcga gcaccagcgc cggcccggtc cagaaccaca gcaccatgat
     1201 cgggacgctc gccagcagcg ccatcaccag cccgcgctgc agcgtcagcc cgaccgcccg
     1261 gccgtcgccg gccccgtgcg cctgcgtcag cagcgggtcc atgccgagcg tcacgcccat
     1321 gccgatgatc agcgtgccca tcgtccacac gccgcccagc gccgcggccg cgagcgcgtc
     1381 gctgccgagc ccgccgacca tgaccatgtc gacgacgccc atcatcatcg tcccgagctg
     1441 ggtggcgacg atcggtgtcg ccagcacgct cagccggcgc agctccgcgg atacctcgga
     1501 caatgacgga cccctcgatc gccgcggcga ccgcggcaac acttgcaagc ccggcgcgag
     1561 cctgtcaacc ccgcgccgcg cggttcatct cggcccgcac ctcgggtaat ccttcacccc
     1621 gtgtcccccc ttcatgtgct gcgaacgccg cgcctgcacc tgcgccccct gaccccggac
     1681 gacatccccg tgatcgcccg ctatcgcgac gaccccgagg tggcccgcca tcaatcctgg
     1741 gatcgctaca ccgaggacga cgcgcgcgcg ctggtcgagc agctcgccgg tggcacgccc
     1801 ggcacgcccg ggcagtggtt tcagtggggt attacgcgcg ccagcgacgg cctgctgctc
     1861 ggcgactgcg gcctgaagat cgtcggcgag gagccgcgcc tgggacagat cggctacacc
     1921 ctcggacgcg cgcaccagcg ccagggctac gccaccgagg ccgtgcgcgc cgtgctcgac
     1981 ctcgccttcc accgcctcca cctgcatcgc gtcagcgcct ccgtcgacgc cgacaacctc
     2041 gcctcgctcg cgttgctgga gcgcctcggc ctgcgccgcg aggcccattt tagaaagtcc
     2101 gagtggttca agggcgcgtg ggtcgacgat gtcatctacg ccatcctcgc gaccgagtgg
     2161 cccgccgcgc cggacagcga acgatcgccg cggagcgcct gaccacatcg tgcccgcttg
     2221 tcgcaccgcg ccagcgtcgc ctcgcccggt cgacccgctt tcgcgggcca gctgaccgtc
     2281 gtcccgctac tgcccggcac gaaggtgcag gtcagcgccg acgcgccgac cgacccggtc
     2341 gtggtgaccg tgccatgatc ctgtggatcc tgcggatcgt cagttgacga cgcactggcc
     2401 cgccgagcag ccgcaggtga cgtccgcgcg cgaggcttgc tgcgggtcgg cgtcctcgca
     2461 cttcggggcg accatcgacg aggcggcgac gcacgtcgcc tccgaccagt aacaggtcgt
     2521 ctgcaccacg cagtccgacg actgcttgca gcgcaggctc tcgggcatct cggagccgag
     2581 gttctccgac ttgagtgccg cggtcggagt cgccttcgga tgacacgcgg tcatcgaccc
     2641 gaggaggccc aacccgatga acccgaaaga gcagatcttt ttaataaagg tacgcacggc
     2701 gtctctcttc ttgcgccgag gcgcggtgtg cggggctgct acggtccacc gggacccgca
     2761 ccgttcgcag gcccggtcgg tgcctgcacg aaccgtgatg catccagtgc atccgatgtg
     2821 tcgcgtcatg ctcgaacgag ccatgcgcca cacatcattt cgatcagtgt ggtggcgatg
     2881 cgcgtccgcg tcaagcgagg cgcgcgccga tcgatcggcc gccgccacga ccaccgccct
     2941 ggcgtgtgac ccgcttgcga cacaaccgcg acgcacggtg acacctcgaa gacccggcga
     3001 tgacggccgc gaaggtgacg gcgacccaca ttgaagtcct cttgccaata cgacccgcga
     3061 cgctcgacac cccggtcttc gacccggaca acaccgtttt ggacccacct tcgccccaga
     3121 agcgggacta cgtgtggtcc accttcctgc cgttcgtcgg cgtgcacttg ctgtgcttcg
     3181 gcgcgctgtg gaccggggcg cgaccggtcg actgggtcgt gtgcggggtg ctctacttcg
     3241 tgcgggtgtt cggcgtcatg gccggttatc accgctattt ctcacaccgc accttcaaga
     3301 ccagccggtg gttccagttc gtgctcgcgc tgctggccga gagctcccta caaaagggcg
     3361 tgctgtggtg ggccgcccat caccgcgtgc accacaagca caccgacgag ccggccgacc
     3421 gccactcgcc gctgcagtac ggcttttggc actcgcacgt gctgtggctg gccgagcgcg
     3481 gcagcgagga gaccgtgcgc gcccgcgtcc gcgacctcga aaaatttccc gagctcgtgt
     3541 ggctcgacaa aaactggatg gtcccaccga tcgccatggg cctcgccacc tggctggcgt
     3601 tcggctggtc gggcctgttc gtcggcttct tcctgagcac cgtgctggcg tggcacgcga
     3661 ccttcgtcgt caactcgctc gcgcacgtgt tcggcacccg ccggtttgcc acccgggaca
     3721 ccagccgcaa caactggttc atcgcgcttt ttaccctcgg cgagggctgg cacaacaacc
     3781 accaccatta catgggcgcc gcgcggcagg gcttttattg gtgggagatc gacatcacct
     3841 actacatcct cctcggcctc gagcgctgcg gcatcgtcca t
//
LOCUS       DASWNJ010002020         4889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_633215, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002020 DASWNJ010000000
VERSION     DASWNJ010002020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633215"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VGB85_33700"
     CDS             <1..700
                     /locus_tag="VGB85_33700"
                     /inference="COORDINATES: protein motif:HMM:NF039450.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ADYC domain-containing protein"
                     /protein_id="HEY0139107.1"
                     /translation="QGTQTTRVGAQLAETILSIDMDALGATKKVKIKSVVQSAVQPGV
                     YFYTVQSEVSPGVFETICLDGAGNPTEAIPLSKAYDPTTATRVVDSGALTWACRGAAL
                     AKAIEWGYVPWTSAAMEDAHEAAVRMIRADYCGDGVTHTTNGNTIDVADKWGIQTPDT
                     NWGIEAKWGPNGAVCLNTPRKLFYPRSSIPCAASLPYCTDNGLANGTPNTSPSQYGGL
                     LVTRAIPNDNPSAY"
     gene            860..3001
                     /locus_tag="VGB85_33705"
     CDS             860..3001
                     /locus_tag="VGB85_33705"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY0139108.1"
                     /translation="MRSLLGSLAALSLLLACAPPNKARPYPTTPPVAGDASAAPSTGL
                     VRYFDRAVDLGPFLTGFPFERWMPSLETDRLFFFATGERYTLKMLDLRGATVPYDLGK
                     ATSVGDVDWSKRSLWSLHHHAASDTLWLHADERNDEQMNLWTLDLKSGLLTQVTRHDY
                     VYGLGFSEDDKQVAYLPRTGTKAPFSTCLRVMDVQTKAAREVVCDTPALRFTWSEIRW
                     SPDASEVYFQAQVEGDRNRVQLVSVDLTAARPTVRVLTDAKTERTDAVALEGFVDDDR
                     LIFIANDDGFANLHAYSRKTRQVKQLTRFTEDVTSARLVDAGVFAVHRTPMGSSLSLV
                     DPRSGKVLGEQPQRGKADILDGHGERVLWTQEAPDLVFELSLATLAPSDGKAAGLGNV
                     RVIGLPEALSAQIVQCRAEAVKIPTFDRVNGKPRELHAFVLHPLRPLADKTRKVALVR
                     SFYGGENSYSTFDHVMCAAGLTVVSPAVRGSDGFGKTFSALNDRDLGGDEIVDLFHVA
                     RWIEKTLGLTPRQIGVYGGSHGGYATMRALTFPPATNNRNDSYAFGFGLSHAGFSDIK
                     NFHDQSNIPDWVVLESGDPSKPDDLARMRDRSPISHVDLLRAPLLLTHGGNDWRVPVG
                     ESRAFHEKAKALGKPTTYVEFAGQGHHIEGLTLLRQLFQARFDFLMAVAQGATPAPAS
                     KPMTTPMTTPTTAAAATAKPATPTATTPPRP"
     gene            complement(3005..4540)
                     /locus_tag="VGB85_33710"
     CDS             complement(3005..4540)
                     /locus_tag="VGB85_33710"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY0139109.1"
                     /translation="MSLSLETGCRGRNLASAMAGGPTVEGQARCGVVKSHARPLIIEW
                     PSADRAALEGLVEKNNGLVAVRYAGCEMEIVRRCQVAGHYGFTAVEPKRDDQEFRSAD
                     ELYAKIPLGAAQLESQLERHGALTLEMTVVGHYETGRERVARSELRGDCDEATHVISA
                     VTVGAFKLSSGSGAKISGGVGVAGFASGGAGSSAERGAERSDGDPARCHGGAASAPPE
                     GCGSLLRLEVEPIREADELAALRERRPCPRGTVLVEGGRFRPSVDGVGVVAVPDFCID
                     RDEVSVSAYASCADDGPCSAAPTTVAYEGLVDKQRAVESRLCNGDRRVRGRHPVNCVS
                     WHQAEAYCRARGGRLPTEHEWEWAARGGDQERSYPWGEEEPGPRLVNACGRECERHFG
                     DAYSDNVPSLHEANDGAVATARVGDYSRGAGRGRVNDLAGNVWEWTASPAITYRTQDE
                     ELPGGGSGRGGPVTGNRVVRGGSFMASEDDELKISARALRNLKDRRADVGFRCAHEPG
                     SGP"
     gene            complement(4655..>4889)
                     /locus_tag="VGB85_33715"
     CDS             complement(4655..>4889)
                     /locus_tag="VGB85_33715"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEY0139110.1"
                     /translation="GEDEFASDTQLMARQLGLTFPVVHDAGNALAGRFRVRQLPATLV
                     LDRKGVVRWQARPGDGVAELRRAVAALREDDAG"
BASE COUNT          717 a         1927 c         1580 g          665 t
ORIGIN      
        1 ccagggcacc cagaccaccc gcgtcggcgc gcagctggcc gagacgatcc tgtcgatcga
       61 catggacgcg ctgggcgcga cgaagaaggt caagatcaag agcgtggtgc agagcgcggt
      121 gcagccgggc gtctattttt ataccgtcca gagcgaggtc tccccggggg tgttcgagac
      181 gatctgcctc gacggcgccg gcaacccgac cgaggcgatc ccgctgtcga aggcctacga
      241 cccgaccacc gcgacgcgcg tcgtcgacag cggggcgttg acctgggcct gtcgcggcgc
      301 cgcgctcgcc aaggcgatcg agtggggcta cgtcccgtgg accagcgcgg cgatggagga
      361 cgcgcacgag gccgccgtcc gcatgatccg cgccgactac tgcggcgacg gtgtcaccca
      421 cacgaccaac ggcaacacga tcgacgtcgc cgacaagtgg gggatccaga cgcccgacac
      481 caactggggc atcgaggcca agtggggccc gaacggcgcc gtgtgcctca acaccccgcg
      541 caagctgttc tacccgcgca gcagcatccc gtgcgcggcc tcgctgccgt actgcaccga
      601 caacggcctc gccaacggca cgcccaacac cagcccctcc cagtacggcg gcctgctggt
      661 cacgcgcgcc atccccaacg acaacccgtc cgcgtactaa aaggcgcccc ggcccggcgc
      721 cccggcgccg aaagctcgcc cctcggccgg ccctcgtcga ccggcacttg tcggccggca
      781 cctgtcggcc ggcacctgtc gaccggcccc tgtcgaccgg ccccttgtca gcggcgccgc
      841 cgcgctgcta cttgggagca tgcgctcgct gctcggttcg ctcgccgcac tgtctctgtt
      901 actcgcgtgc gccccgccga acaaggccag gccctacccg accacgccgc cggtcgccgg
      961 tgacgcctcc gccgcgccga gcactggcct ggtccgctac ttcgaccgcg ccgtcgacct
     1021 cgggccgttc ctgaccggct tccccttcga gcgctggatg cccagcctcg agaccgaccg
     1081 cctgttcttc ttcgccaccg gcgagcgcta caccctcaag atgctcgacc tgcgcggcgc
     1141 cacggtcccc tacgacctcg gcaaggcgac gagcgtcggc gacgtcgact ggtcgaagcg
     1201 cagcctgtgg agcctgcacc accacgccgc ctccgacacc ttgtggttgc acgccgacga
     1261 gcgcaacgac gagcagatga acctgtggac gctcgacctg aagtcgggcc tgctgacgca
     1321 ggtcacccgc cacgactacg tgtacggcct cggcttctcg gaggacgaca agcaggtcgc
     1381 ctacctgccg cgcaccggca ccaaggcgcc gtttagcacc tgcctgcggg tgatggacgt
     1441 gcagacgaag gccgcgcgcg aggtcgtgtg tgacaccccg gcgctgcgct tcacctggtc
     1501 ggagatccgc tggtcgccgg acgcgagcga ggtctacttt caggcccagg tcgagggcga
     1561 ccgcaaccgc gtgcagctgg tctcggtcga cctgaccgcc gccaggccga ccgtccgcgt
     1621 gctgaccgac gcgaagaccg agcgcaccga cgccgtcgcg ctcgagggct tcgtcgatga
     1681 cgaccgcctg atcttcatcg ccaacgacga cggcttcgcc aacctccacg cgtattcacg
     1741 caagaccagg caggtcaagc agctgacccg cttcaccgag gatgtcacct ccgcgcggct
     1801 ggtcgacgcc ggtgtgttcg ccgtgcaccg cacaccgatg ggctcctcgc tgtcgctcgt
     1861 cgacccgcgc agcggcaagg tcctcggcga gcagccgcag cgcggcaagg ccgacatcct
     1921 cgacggtcac ggcgagcgcg tgttgtggac gcaggaggcg cccgacctgg tgttcgagct
     1981 cagcctcgcc acgctcgcgc cgagcgacgg caaggcggcc ggcctcggca acgtgcgcgt
     2041 gatcggcctg cccgaggcgc tgtcggccca gatcgtccag tgccgcgccg aggccgtcaa
     2101 gatcccgacc ttcgaccgcg tgaacggcaa gccgcgcgag ctgcacgcct tcgtcttgca
     2161 cccgctgcgc ccgctcgccg acaagacgcg caaggtggcg ctggtgcgct ccttctacgg
     2221 cggcgagaac agctacagca cgttcgatca tgtcatgtgc gcggccggcc tcaccgtcgt
     2281 ctcgcccgcg gtgcgcggca gcgacggttt tggcaagacc tttagcgccc tcaacgaccg
     2341 cgacctcggc ggcgacgaga tcgtcgacct gttccacgtc gcgcgctgga tcgagaagac
     2401 cctcggcctc acgccgcgcc agatcggcgt gtacggcggc agccacggcg gctacgcgac
     2461 catgcgcgcg ctgaccttcc cgccggcgac caacaaccgc aacgacagct acgccttcgg
     2521 cttcggcctc agccacgccg gcttctccga catcaagaac tttcatgatc agtccaacat
     2581 cccggactgg gtcgtgctcg agagcggcga cccctcgaag ccggacgacc tcgcccgcat
     2641 gcgcgaccgc agcccgatct cccacgtcga cctgctgcgc gccccgctgc tgctcaccca
     2701 cggcggcaac gactggcgcg tcccggtcgg cgagtcgcgg gcctttcatg agaaagccaa
     2761 ggcactcggc aagccgacca cgtatgtgga attcgccggc cagggccacc acatcgaggg
     2821 cctgaccctg ctgcgccagc tgttccaggc ccgcttcgac ttcctgatgg ccgtcgcgca
     2881 gggcgccaca ccggcgcccg cctcaaagcc gatgaccacg ccgatgacca cgccgacgac
     2941 cgccgcggcc gcgaccgcca agcctgcgac cccgaccgcg accaccccgc cgcggccctg
     3001 acgctcacgg cccgctgcca ggctcatgcg cgcagcggaa ccctacgtcg gcgcgacggt
     3061 ccttgaggtt ccgcagcgcc cgcgcggaga tcttcagctc atcatcctcg ctggccatga
     3121 agctgccgcc gcgcacgacc cggttgccgg tgaccggtcc gccgcggccc gagccgccgc
     3181 cgggaagctc ctcgtcctgc gtgcggtacg tgatcgccgg gctcgcggtc cactcccaca
     3241 cgttgcccgc gaggtcgttg acgcggcctc ggccggcgcc gcgcgaataa tcgccgaccc
     3301 tcgccgtcgc caccgcgccg tcgttcgcct cgtgcaggct cggcacgttg tcgctgtacg
     3361 cgtcgccgaa gtgacgctcg cactcgcggc cgcacgcgtt caccaggcgc gggcccggct
     3421 cctcctcgcc ccacggataa ctgcgctcct ggtcgccacc gcgggccgcc cactcccact
     3481 cgtgctcggt cggcaacctg ccaccacgcg cccggcagta cgcctccgcc tgatgccagc
     3541 tgacgcagtt gaccgggtgg cgcccgcgga cccggcggtc gccgttgcac agccggctct
     3601 cgaccgcccg ctgcttgtcc accagcccct catacgccac cgtcgtcggc gcggccgagc
     3661 acggcccgtc gtcggcgcag ctcgcatacg cggagacgct gacctcgtcg cggtcgatgc
     3721 agaagtcggg caccgcgacg acgccgacgc cgtcgacgct cggccggaac ctgccgccct
     3781 cgacgagcac cgtgccgcgc ggacacggcc ggcgctcgcg cagcgccgcc agctcgtcgg
     3841 cctcgcggat cggctcgacc tcgaggcgca gcagggaccc gcagccctcc ggcggagccg
     3901 acgccgcgcc gccgtggcag cgcgccggat ctccgtcact gcgctcggcc ccgcgctccg
     3961 ccgaggaccc cgccccgccg cttgcgaagc cggccacccc gacgcccccc gagatcttcg
     4021 cacctgaccc cgaggacagc ttgaacgcgc cgaccgtgac cgccgagatc acgtgggtcg
     4081 cctcgtcgca gtcgccgcgc agctcgctgc gggcgacccg ctcgcggccg gtctcgtagt
     4141 gaccgacgac cgtcatctcg agggtcagcg cgccgtggcg ctcgagctgg ctctcgagct
     4201 gcgcggcccc gagggggatc ttcgcgtaca gctcgtcggc gctgcgaaac tcctggtcgt
     4261 cgcgtttggg ctcgaccgcc gtgaagccgt agtgcccggc gacctggcag cggcgaacga
     4321 tctccatctc gcagccggcg tagcgcaccg cgaccagccc gttgttcttc tcgaccaggc
     4381 cctcgagcgc cgcgcggtcg gccgacggcc actcgatgat cagcggccgc gcgtggctct
     4441 tgaccacccc gcagcgcgcc tggccctcga ccgtcggccc gcccgccatc gccgaggcga
     4501 ggttgcgccc gcggcaacct gtctccaggg acaggctcac aagtactacc cagcacgcgt
     4561 gcagcgccca aaccgaggga gatcgaggcg agttcggcac gagaccagcg taccggctcc
     4621 ccgtctcgcg gggaagcccg cgacgcggcg cttctcagcc ggcgtcgtcc tcgcgcaggg
     4681 ccgcgacggc gcggcgcagc tcggcgacgc cgtcgccggg tcgggcctgc cagcggacca
     4741 cgcctttgcg gtcgaggacc agcgtcgccg gcaattgccg gacccggaag cggccggcga
     4801 gcgcgttgcc ggcgtcgtgc acgaccggga aggtgaggcc gagctggcgg gccatcagct
     4861 gtgtgtcgct ggcgaactcg tcctcgccg
//
LOCUS       DASWNJ010002021         3058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_633218, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002021 DASWNJ010000000
VERSION     DASWNJ010002021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633218"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..>3058
                     /locus_tag="VGB85_33720"
     CDS             248..>3058
                     /locus_tag="VGB85_33720"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0139111.1"
                     /translation="MGPVRRQGQQGDTGDVEARRMYAKVTERLFGEPSAPVRIGRYRV
                     VERLGSGAMGVVYSAVDERLGRKLAIKLLHESFNGDPTGRARLLREAQAMARLRHENV
                     VLVYDVGTHGDGDEQVFIAMELVEGRTLELWRREAPRSVDEILACYIQAGKALAAAHR
                     AGVLHRDFKPENVLVDAAGRVRVLDFGLARALAGPAEGDPADSLSEPLTRTGSILGTP
                     AYMAPEQLRGESTDARADQFSFCVALYEALYDERPFGTVPRFAAVEVTHVPKDSRVPA
                     HLRRALLRGLADAPADRHADMDALLTALRPTVASRRALVAGLLVVGGAGLGAWAAVSL
                     SGNPGLDFAALHGSIEQDLWVHEAHTRLEAASQGSAEVRDSRRVARFHPLLAQDPTSA
                     ALLLRELAPGVEGLAAMRRQVLARPLTRFSVPAEGVRALSFAADVLWIADAGGTRSFT
                     ADGRPGPGTPPTPAAPTPADATQWPSPDGRSVLRSDRHGRVFIDRTALREHEGDIGAA
                     AWSPDGQRVAVAGEPGIHVWDRRGKLRRRIDGHEGAVRVLAWSPTGADLLTGGDDGQI
                     RILRVAGTDPGTRVLRGHTGPITALAWHPGGDRIASASADGSVRVWSLVPGARVRHDH
                     PAAVQALAWHPGGELLASAADDGKIHLFSLATAAPPRTLEHGRSVLSIAWSPDGTRLA
                     SAAGGDGNVRVWSDTGELLHPLPTHGRRGEEPLAVAWTPAGDLLAACNDERVLLWSGG
                     QGPAHPVAGPRTALMRLAFRPGSTDLATSSVDGAARRWTLDLSGETHALREVGRLDGE
                     VNDLAWCLDGALALAREDRRVDLLAAGGTSLLLQRRTGAAATSVACDPGGGLLVTGLA
                     DGRIRLVSRDGATDPADLTGHTAAVDVLAFGPRGDALASADRAGHLYVWPWTDAALAE
                     QLRVATWVCLTPSQRSSLLDEPP"
BASE COUNT          365 a         1274 c         1035 g          384 t
ORIGIN      
        1 ctgcaccagc ttgccgccgg ccgcgcgatc gcccgcgcgc caggccgtca acagcgtcca
       61 gtcgtcgtcg cggccgtcca tcacgcgcag cataacgccc gcggcccccc tcgacgaagc
      121 gatgtcggca ttaggctctg attcgtcggt gatttcggtt accgaaattt cacatgcagt
      181 gcgggttgtt gggaatcacc tcgacccccg gtcggcgcga cgatcttgct atggtggccg
      241 cgcggcgatg ggaccggtgc gcaggcaggg acagcagggc gacaccggcg acgtcgaggc
      301 gcgccgcatg tacgcgaagg tcaccgagcg gctgttcggt gagccgagcg cgccggttcg
      361 catcggtcgc taccgggtcg tcgagcgcct cggctccggg gcgatgggcg tcgtctacag
      421 cgcggtcgac gagcgcctcg gtcgcaagct ggcgatcaag ctgctgcacg agtcgttcaa
      481 cggcgacccg accggccgcg cccggctgct gcgcgaggcc caggccatgg cccgcctgcg
      541 ccacgagaac gtcgtcctcg tgtacgacgt cggcacccac ggcgacggcg acgagcaggt
      601 cttcatcgcc atggagctcg tcgagggccg cacgctcgag ctgtggcgcc gcgaggcccc
      661 gcgctcggtc gacgagatcc tcgcctgcta catccaggcc ggcaaggccc tcgccgcggc
      721 ccaccgcgcc ggcgtcctgc accgcgactt caagcccgag aacgtgctcg tcgacgccgc
      781 cggccgcgtc cgcgtcctcg acttcggcct cgcccgcgcc ctcgccgggc ccgccgaggg
      841 cgaccccgcc gactccctct ccgagccgct cacccgcacc ggcagcatcc tgggcacccc
      901 cgcctacatg gccccggagc agctgcgcgg cgaatccacc gacgcccgcg ccgaccagtt
      961 cagcttctgc gtcgccctct acgaggcgct gtacgacgag cgcccgttcg gcaccgtccc
     1021 gcgcttcgcc gccgtcgagg tcacacatgt ccccaaggac agccgcgtgc ccgcccacct
     1081 gcgccgcgcc ctcctgcgcg gcctcgccga cgcccccgcc gaccgccacg ccgacatgga
     1141 cgcgctgctc accgcgctgc gtccgaccgt cgcctcgcgg cgggccctcg tcgccggcct
     1201 gctggtggtc ggcggcgccg gcctcggcgc gtgggcggcc gtctcgctga gcggcaaccc
     1261 cggcctcgac ttcgccgccc tgcacggcag catcgagcag gacttgtggg tccacgaggc
     1321 ccacacgcgt ctcgaggccg cctcgcaggg ctccgccgag gtccgcgaca gccgccgcgt
     1381 cgcccgcttc cacccgctgc tcgcccagga cccgacctcc gccgccctcc tgctgcgaga
     1441 gctcgccccc ggggtcgagg gtctcgccgc gatgcgccgc caggtgctcg cccgcccgct
     1501 gacccgcttc agcgtccccg ccgagggcgt gcgcgcgctg tcgttcgccg ccgatgtgtt
     1561 gtggatcgcc gacgccggcg gcacccgcag cttcaccgcc gacggccggc ccggccccgg
     1621 caccccaccg acgccggccg ccccgacgcc cgccgacgcg acgcaatggc cgagccccga
     1681 cggccgcagc gtcctgcgca gcgaccgaca cgggcgcgtg ttcatcgacc ggaccgcgct
     1741 gcgcgagcac gagggtgaca tcggcgccgc ggcgtggagc cccgacggcc agcgcgtcgc
     1801 cgtcgccggg gagccgggga tccacgtatg ggaccgccgt ggcaaattgc gccgccggat
     1861 cgacggccac gagggggccg tccgcgtgct cgcctggagc ccgaccggcg ccgacctgct
     1921 gaccggcggc gacgacggcc agatccggat ccttcgcgtc gctggcaccg accccgggac
     1981 ccgggtcctg cgcggccaca ccgggccgat caccgcgctc gcctggcacc ccggcggcga
     2041 ccggatcgcc agcgccagcg ccgacggcag cgtgcgcgtg tggtcgctcg tcccgggcgc
     2101 ccgcgtccgt cacgaccacc ccgccgccgt gcaggcgctc gcctggcacc ccggcggcga
     2161 gctcctggcc agcgccgccg acgacggcaa gatccacctg ttctcgctcg ccaccgccgc
     2221 gccgccgcgc acgctcgagc acggccgcag cgtgctctcg atcgcctgga gccccgacgg
     2281 cacccggctc gccagcgccg ccggcggcga cggcaacgtg cgcgtgtgga gcgacaccgg
     2341 cgagctcctg cacccgctgc cgacccacgg ccgtcgcggc gaggagccgc tcgccgtcgc
     2401 ctggaccccc gcgggcgatc tcctggccgc ctgcaacgac gagcgcgtgc tgctgtggtc
     2461 cggcggtcag ggtccagcgc acccggtcgc cggcccgcgg accgcgctga tgcgcctggc
     2521 ctttcgacca ggctccaccg acctggcgac cagcagcgtc gacggcgcgg cccgccgctg
     2581 gaccctcgac ctgtccggag agacacacgc cctgcgcgag gtcggccgcc tcgacggcga
     2641 ggtcaacgac ctcgcctggt gcctcgacgg tgcgctcgcg ctcgcccgcg aggaccgccg
     2701 cgtcgatctg ctcgccgcgg gcgggacttc cttgctgctg caacgacgca cgggggccgc
     2761 agccaccagc gtcgcctgtg accccggcgg cggtctgctc gtcaccggcc tcgccgacgg
     2821 ccggatccgc ctggtgtccc gcgacggcgc gaccgatccc gctgacctga ccgggcatac
     2881 cgccgccgtc gatgtcctcg ccttcggccc ccgcggcgac gccctcgcca gcgccgaccg
     2941 tgccggccac ctttatgtat ggccatggac cgacgccgcg ctcgccgagc aacttcgcgt
     3001 cgcgacctgg gtgtgtctga ccccgtcgca gcgctcgagc ctgctcgacg agccgccg
//
LOCUS       DASWNJ010002022         2745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_348813, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002022 DASWNJ010000000
VERSION     DASWNJ010002022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348813"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="VGB85_33725"
     CDS             <1..1360
                     /locus_tag="VGB85_33725"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY0139112.1"
                     /translation="SAPAPVAELRTPIGYRVAKPALVVHVQPHKRRGRRGRIEGRQPF
                     AIYGLRAGPDCEGEGWAEVDQGGFVCLEHAVASAGPATIQPKLLAGADVPFIYARPKV
                     LDRRSEAIAEVPRWRDRLSHARGEPPIDVLGPDRQYTFVETKLRRGGLILIDAEERAV
                     PAADMKIAKPSRFAGRELASAPIPAGLHAAWAVSHPATLRERAEDDAKPVHYIRYHSS
                     LHIDPAPIQVGEATWYAVPDGGGPGVVAYVISEDVNHWIPGPTLAVQPDEYWLDVELG
                     QQTLAVMQGDAAIFITLISSGAGGFATPTGLFRIYEKLAVSTMKSSPSSEDPYFVEGV
                     PWIQYFHRRYAFHASYWHDDFGKRRSHGCVNLSPKDAAYVFGLTHPVVPPGWNSLYEH
                     AGDAGTLVRVRRGTDPVPDLRLPLGVIESDDEKTDGTLEKTADAPDAPDAPDAPDAPA
                     AP"
     gene            complement(1370..1822)
                     /locus_tag="VGB85_33730"
     CDS             complement(1370..1822)
                     /locus_tag="VGB85_33730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139113.1"
                     /translation="MSTRFQSVEVPAQLQHLRRKCWRVVLVLDCVSAPLCALAVATLP
                     DPGRPLVALRALQQAAAKLRGVRHASHLRAHEAARDAASAALADADSDDMLLDQIVAD
                     LTAHARGDTTSLDWLDPSDIDPPLWSAYVSAVQATHRALWMARQDIPG"
     gene            2094..>2745
                     /locus_tag="VGB85_33735"
     CDS             2094..>2745
                     /locus_tag="VGB85_33735"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEY0139114.1"
                     /translation="MQPQPPAVRPPLDQRSFPLPDARLIVIATSAGGLIAIGRVLAAL
                     PVDFPAAIAIVQHRGLQHPELLPELLGKWTALPVRHARSGEVFTAGVVYVCPPGMHMT
                     VEHCVRLVEEPRINAVRPSADLMLRSAARNYGERSIGVVLSGTGRDAALGCLAIAQAG
                     GTVIVQEASSCEFPGMPGAAAQLVPTELVLPPEQIGPLLLQLVAGRPLARRPVVRAA"
BASE COUNT          394 a          950 c          993 g          408 t
ORIGIN      
        1 ctccgcgccc gcgccggtcg ccgagctgcg cacgccgatc gggtatcgcg tcgccaagcc
       61 ggcgctcgtc gtgcacgtgc agccgcacaa gcgacgcggc cgccgcgggc gcatcgaggg
      121 ccgccagccg ttcgcgatct atggcctgcg cgctggcccc gactgcgagg gtgagggctg
      181 ggccgaggtc gatcagggcg gctttgtctg cctcgagcac gcggtcgcca gcgcgggacc
      241 ggcgacgatc cagccgaagt tgctcgccgg cgccgacgtg ccgttcatct acgccaggcc
      301 caaggtgctc gaccgccgca gcgaggcgat cgccgaggtc ccgcgctggc gcgaccgcct
      361 ctcgcacgcg cgcggcgagc cgccgatcga cgtgctcggg ccggatcgcc agtacacctt
      421 cgtcgagacc aagctgcgca ggggcgggct gatcctgatc gacgcggagg agcgcgcggt
      481 gccggcggcc gacatgaaga tcgccaagcc gagccgcttc gccgggcgcg agctggccag
      541 cgcgccgatc cccgcgggcc tgcacgccgc gtgggcggtc agccatccgg cgacgctgcg
      601 cgagcgcgcc gaggacgacg ccaagccggt gcactacatc cgctaccact cgtcgctgca
      661 catcgacccc gcgccgatcc aggtcggcga ggcgacctgg tacgcggtgc cggacggcgg
      721 cggcccgggt gtcgtcgcct acgtgatctc cgaggacgtc aatcactgga tccccggccc
      781 gacgctcgcg gtccagcccg acgagtactg gctcgacgta gagctcgggc agcagacgct
      841 cgcggtgatg cagggcgatg cggcgatctt catcaccctg atctcgagcg gcgccggcgg
      901 cttcgccacg ccgaccgggc tgtttcgcat ctacgaaaag ctcgcggtgt cgacgatgaa
      961 gagcagcccg agctcggagg acccgtactt cgtcgagggc gtgccgtgga tccagtactt
     1021 tcatcgacgc tacgcgtttc atgcctcgta ctggcacgat gattttggta agcgccgcag
     1081 ccacggctgc gtcaacctct cgcccaaaga cgccgcgtac gtgttcgggc tgacgcaccc
     1141 ggtggtgccg cccggctgga actcgctcta cgagcacgcg ggcgacgcgg gcacgctggt
     1201 ccgggtgcgc cgcggcaccg acccggtgcc cgacctgcgc ctgccgctcg gggtgatcga
     1261 gtccgacgac gagaagaccg acggcacgct cgagaagacc gcagacgcgc cggatgctcc
     1321 ggatgctccg gatgctccgg atgctccggc agcgccgtga gtcgggatct cagcccggga
     1381 tgtcctggcg ggccatccac agggcgcggt gggtcgcctg cacggcgctg acgtaggcgg
     1441 accacagggg cgggtcgatg tccgaggggt cgagccagtc gagcgaggtc gtgtcgccac
     1501 gcgcgtgcgc cgtcaggtcg gcgacgatct ggtcgagcag catgtcgtcg ctgtcggcgt
     1561 ccgccagcgc cgcgctcgcg gcgtcgcggg cggcctcgtg ggcgcgcagg tgcgaggcgt
     1621 gtcgcacgcc ccgcagcttc gccgccgcct gctgcaacgc ccgcaacgcg accagcggac
     1681 gacccgggtc ggggagcgtg gcgacggcga gcgcacacag gggcgcgctg acgcagtcga
     1741 ggacgaggac cacgcgccaa cacttgcggc gcaggtgctg tagctgtgcg gggacctcga
     1801 ccgactgaaa gcgcgtactc atgccgtcaa catgaacgca gttcggtaaa tgtgtagtaa
     1861 ggaaaagccc gtgtggtgaa aagttgtcag tgcatcgaac atgcgcggtt ggtgtatccg
     1921 ggaagacacc ggcgtccatc cggggtcgcc gtgtgagcct gcggcgctgt cgctataacc
     1981 gaccgatgtt ggtcgttttc gtcggtttgc aaggaaataa ctggcggatc cggagaggcg
     2041 gccgtcggag atgcgggtaa accctgctcc ctggttgtag tatccacggg cgcatgcagc
     2101 cgcaacctcc cgccgttcgc ccgcctctcg accagcggag ctttcctctg cccgacgccc
     2161 ggctgatcgt gatcgcgacc tcggcgggag gattgatcgc gatcggtcgg gtgctcgccg
     2221 cgctgcccgt ggattttccg gccgcgatcg cgatcgtaca acaccgcggc ttgcaacacc
     2281 cggagctgct gccggagctg ctcggcaagt ggaccgcgct gccggtccga cacgcgcgct
     2341 cgggcgaggt gttcacggcc ggcgtcgtct acgtgtgtcc gccgggcatg cacatgacgg
     2401 tcgagcactg cgtccgcctc gtcgaggagc cgcggatcaa cgcggtgcga ccgagcgccg
     2461 acctgatgct gcgctcggcg gctcgcaact acggcgagcg cagcatcggt gtcgtgctgt
     2521 cgggcaccgg ccgcgacgcc gcgctcggct gcctggcgat cgctcaggcc ggcggcacgg
     2581 tcatcgtcca ggaggcgagc agctgcgagt tccccgggat gccaggcgcc gcggcccagc
     2641 tggtgccgac cgagctggtg ctgccgccgg agcagatcgg gccgctgctg ttgcagctgg
     2701 tcgccggccg gccgctcgcg cgtcgtccgg tcgtgcgcgc cgcgt
//
LOCUS       DASWNJ010002023         3322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_420371, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002023 DASWNJ010000000
VERSION     DASWNJ010002023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420371"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..2024
                     /gene="cysN"
                     /locus_tag="VGB85_33740"
     CDS             120..2024
                     /gene="cysN"
                     /locus_tag="VGB85_33740"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF004035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HEY0139115.1"
                     /translation="MEPGMENLTQHSLLRFITAGSVDDGKSTLIGRLLFDSKGIFADQ
                     LAAVSRSKHKRTVGDTIDLSLLTDGLEAEREQGITIDVAYRYFSTDRRKFIIADCPGH
                     EQYTRNMATGASNCHLAIILIDARHGVMPQTRRHSFICALLGIKHIVVAINKMDLVGY
                     DEARYDQIKQDYTDFIAKLEVHDLSFIPMSALTGENVVDPAPSMPWYLGPPLLAHLES
                     VHIASDHNLADLRFPVQLVQRPSHDFRGFAGTIASGVLRRGDEVVVLPSRARTRIDRI
                     MTFDGDLEQAQAPMAVTLTLADEVDVSRGDMLCHPDNLPHQSDQVEAMIVWMAEKPLV
                     PGRSYLLKQTTRVVPAELATLHHAIDVGSLERRPASSLAMNEVGRVTLALHRPIFLDP
                     YKINRATGAFIIVDRSSNGTVGAGMLLDHTETADNAADPWTAPGVDEGPQDPGSAITP
                     QERAARFGQRPVTLLLTGLPASGKATLAYALERRLFDAGRAVTVLYGPAMRRGLSRDL
                     GYSVDDRSENLRRSAEVARLVNNAGLLCICAFVAPHAAVRQRVRQLLGDERVLEVYLS
                     APAEVCRQRDRTGMYARAERGELKHFPGVSAAYEPPERPDLVLPTDTLGPDECVEQVL
                     ALLRQRGLLT"
     gene            2058..3065
                     /locus_tag="VGB85_33745"
     CDS             2058..3065
                     /locus_tag="VGB85_33745"
                     /inference="COORDINATES: protein motif:HMM:NF024569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 family protein"
                     /protein_id="HEY0139116.1"
                     /translation="MPFRSDDHAPTGVYVPADPLARFAEIHAALDSERKWHGDKIPLR
                     LAAVCLITTPGEPAQLASATRRRDADLRSGLGWLSNVTGAVRMLIAAQMVKHDDDPVT
                     FIDEVERVRALMRAAKVRRDAVYETLAVLVLRRVLGQQRIEAAHIERFHAIYEAMKRH
                     HWFLTGPEDYPACAMLLARSEDPQAIGDGTDAIYKALHDRADLWRGDALQTAANVLYL
                     SGVEPVAIADRFAALMNGFRAGGARIGQDQYDELAILCFLAWPVERIVQAVLEFRTQL
                     LETQKWIGKDGALNLAASLAFIRIAGQDSALGPLADAKLLLDMQAIVAARQAAAVVAA
                     S"
BASE COUNT          537 a         1289 c         1019 g          477 t
ORIGIN      
        1 attgccgaaa ccgcgatcac ccaggtcacg gaacgcggcg ccacccgcat ggacgaccag
       61 acctcggaag cgtcgatgga aaaacgcaag aagcaaggat atttctgatg aacgcacgca
      121 tggaacccgg tatggaaaat ctgacccaac acagcctgct gcgcttcatc accgccggct
      181 ccgtcgacga cggcaagagc accctgatcg gccgcctgct gttcgacagc aagggcatct
      241 tcgccgacca gctggccgcc gtgtcgcgct cgaagcacaa gcgcaccgtc ggcgacacca
      301 tcgacctgtc gctgctgacg gacggcctgg aagccgagcg cgagcaaggc atcacgatcg
      361 acgtcgccta ccgctacttc agcaccgacc ggcgcaagtt catcatcgcg gactgcccgg
      421 gccacgagca gtacacgcgc aacatggcga ccggcgccag caattgccac ctggcgatca
      481 tcctgatcga cgcgcgccac ggtgtcatgc cgcagacccg ccgccacagc tttatctgcg
      541 cgctcctggg catcaagcac atcgtggtcg cgatcaacaa gatggacctc gtcggctacg
      601 acgaggcccg ctacgaccag atcaagcagg actacaccga cttcatcgcc aagctcgagg
      661 tccacgacct cagcttcatc ccgatgtcgg cgctgaccgg cgagaacgtg gtcgaccccg
      721 cgccgagcat gccgtggtac ctcggcccgc ccctgctcgc ccacctcgaa tcggtgcaca
      781 tcgcctccga tcacaacctc gccgacctgc gcttcccggt gcagctggtc cagcgcccga
      841 gccacgactt tcgcggcttc gccggcacga tcgcctcggg cgtgctgcgc cgcggcgacg
      901 aggtcgtcgt tttgccctcg cgcgcgcgca cccgcatcga ccgcatcatg acctttgatg
      961 gcgacctcga gcaggcccag gcgccgatgg ccgtcacgct gacgctcgcc gacgaggtcg
     1021 acgtcagccg cggcgacatg ctgtgccacc ccgacaacct cccgcaccag tccgaccagg
     1081 tcgaggcgat gatcgtgtgg atggccgaaa agccgctcgt ccccggccgc agctatctgc
     1141 tgaaacagac cacgcgcgtc gtgccggccg agctcgccac gctgcaccac gcgatcgacg
     1201 tcggctcgct cgagcgtcgt ccggcctcca gcctggcgat gaacgaggtc ggccgcgtca
     1261 ccctcgcgct gcaccgaccg atcttcctcg acccgtacaa gatcaaccgg gccaccggcg
     1321 ccttcatcat cgtcgaccgc agcagcaacg gcaccgtcgg cgccggcatg ctgctcgacc
     1381 acaccgagac cgccgacaac gcggccgacc cctggaccgc ccccggcgtc gacgagggcc
     1441 cgcaggaccc cggcagcgcg atcaccccgc aagaacgcgc cgcccgcttc ggccagcggc
     1501 ccgtcaccct gctgctgacc ggcctgcccg cgtccggcaa ggcgaccctc gcctacgcgc
     1561 tcgagcgccg cctgttcgac gccggccgcg cggtcaccgt gctctacggc ccggcgatgc
     1621 gtcgcggcct ctcgcgcgac ctcggctact ccgtcgacga ccgcagcgag aacctgcgcc
     1681 gcagcgccga ggtcgcccgc ctggtcaaca acgccggcct gctgtgcatc tgcgccttcg
     1741 tggccccgca cgcggccgtg cgccagcgcg tgcgccagtt gctcggcgac gagcgggtgc
     1801 tcgaggtcta cctctcggcc ccggccgagg tctgccgcca gcgcgaccgg accggcatgt
     1861 acgcgcgcgc cgagcgtggc gagctcaagc acttcccggg cgtcagcgcc gcgtacgagc
     1921 ccccggagcg cccggacctc gtgctcccga cggacaccct cgggcccgac gagtgcgtcg
     1981 agcaggtgct cgccctgctg cgccagcgcg gcctgctcac ctgacctgcg accccgccga
     2041 tcggctatgt tcgccgcatg ccctttcgca gcgacgatca cgcgccgacc ggcgtctacg
     2101 tgcccgccga ccccctcgcg cgcttcgccg agatccacgc cgccctcgac agcgagcgca
     2161 agtggcacgg cgacaagatc ccgctgcggc tcgccgcggt gtgcctgatc accacccccg
     2221 gtgagcccgc gcagctggcc tccgcgaccc gtcgccgcga cgccgacctg cgatccggct
     2281 tggggtggct cagcaacgtc accggcgccg tgcggatgtt gatcgccgcc caaatggtca
     2341 agcacgacga cgaccccgtc accttcatcg acgaggtcga gcgcgtgcgg gccctgatgc
     2401 gcgccgccaa ggtccgccgc gacgccgtct acgagaccct cgccgtgctc gtgctgcgcc
     2461 gcgtgctcgg ccagcagcgc atcgaggcgg cccacatcga gcgcttccac gcgatctacg
     2521 aggcgatgaa gcgccaccac tggttcctga ccgggcccga ggactacccg gcctgcgcga
     2581 tgctcctcgc tcgctccgag gatccgcagg cgatcggcga cggcaccgat gcgatctaca
     2641 aggcgttgca cgaccgggcc gacctgtggc gcggcgacgc cctgcagacc gccgcgaacg
     2701 tcctctatct cagcggcgtc gagcccgtgg cgatcgccga ccgcttcgcc gcgctgatga
     2761 acggctttcg tgccggcggc gcccgcatcg gccaggacca gtacgacgag ctggcgatct
     2821 tgtgcttcct cgcctggccc gtcgagcgca tcgtgcaggc cgtcctggag ttccgcacgc
     2881 agttgctgga gacccagaag tggatcggca aggacggcgc cctcaacctc gccgccagcc
     2941 tcgcgttcat ccgcatcgcc ggccaggaca gcgcgctggg ccccttggca gacgccaagc
     3001 tgttgctgga catgcaggcg atcgtcgccg cccgccaggc cgcggcggtc gtcgcagcgt
     3061 cctgagaaaa tacctccggg cctgcgggcc cggtcaatct tgcgcacata acaaagtcct
     3121 gcgattattt tcgcggggcc gcttgacatg cccaaaagcc ttgggtagta atgctgacct
     3181 gccatttgag gtgaaccatg cttcccgtcc agcacacgca gcttcgcgcc aagaccgacc
     3241 tcgccgcccc ctcgctggcg acgcgtctgt tcgcgaatgc cggcggcctg gagcggtcgt
     3301 gtggcgacgc cccctggatc ag
//
LOCUS       DASWNJ010002024         2758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_420678, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002024 DASWNJ010000000
VERSION     DASWNJ010002024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420678"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="VGB85_33750"
     CDS             <1..902
                     /locus_tag="VGB85_33750"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0139117.1"
                     /translation="LPRQIRNDAVIAKRYRIERVLGAGGMGVVAAAEDVKTGTPVAIK
                     FLIHHAAGEYDGLLERFVREAQALLRVQSEHVVRVLDVGNLANGAPFIVMERLEGEDL
                     NAALHSRGPLSPEEVVDALAQACIAVAAAHACGIIHRDLKPANLFMTRQPNGAGVLKV
                     LDFGVSKLGEEGAPELTQASTMVGSAYYMSPEQMNGMRELDGRSDIWSLGVTAYELLT
                     GKLPFDGSSIAEVCAQVLYEPPKSPLLLRPDLPPALCDVVLRCLSKDREQRHANADEL
                     AEALLGSLPAQATTSPFTRRSAR"
     gene            1024..1233
                     /locus_tag="VGB85_33755"
     CDS             1024..1233
                     /locus_tag="VGB85_33755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139118.1"
                     /translation="MRAPDDTDPIDPLAPRADDPLMFGRRWMWMARLYDRPADGTTRL
                     RTSAVILVMVLVLIVVSLGVVLLTG"
     gene            complement(1223..>2758)
                     /locus_tag="VGB85_33760"
     CDS             complement(1223..>2758)
                     /locus_tag="VGB85_33760"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEY0139119.1"
                     /translation="QVARRLKALRACDNPLERYAFLRDLQDVNETLFYALLVRYAPEL
                     LPIVYTPTVGEGCQRFSEIWRRPRGLFLSYPNRHRIADMFASPRYDRVRVIVVSDGER
                     ILGLGDQGTGGMGIPIGKLSLYTACAGIHPERTLPIFLDVGTDNPDRLNDPMYVGWRS
                     RRIRGAEYDDFVERFIVAVQQRWPDALLQWEDFANQNAGRLLDAYRDRLCSFNDDIQG
                     TAAVAAGTLLSAMAVTRVPLTEQRIAIVGAGSAGCGIAALLLRAMIAAGLSEAAARER
                     FYALDRDGLLVEGMPGIRPAQEPFVQRRQAVAGWTRENPDFIGLVDVARNARPTVLIG
                     VSGQPGAFSEAAIRAMAASVERPVIFPLSNPTSRAEAVPDDLMRWTDGRALIGTGSPF
                     APVPWGGREVAIAQTNNSYVFPGVGLGILASGARRVTDAMFTAAARALATLSPALADP
                     TAPLLPAVSELRRVAVVVAQAVGLQAQADGVAEPCSPDELARRITAQVWEPEYRPYRR
                     VSR"
BASE COUNT          391 a          986 c         1015 g          366 t
ORIGIN      
        1 agctgccgcg acagatccgc aacgacgccg tgatcgcgaa gcgctatcgc atcgagcggg
       61 tgctcggggc cggcggcatg ggcgtcgtcg cggccgcgga ggacgtcaag accggcacgc
      121 cggtggcgat caagttcctc atccaccacg ccgcaggcga gtacgacggc ctgctcgagc
      181 gcttcgtgcg agaggcccag gcgctgctgc gcgtgcagag cgagcacgtc gtccgcgtgc
      241 tcgacgtcgg taacctcgcc aacggcgcgc cgttcatcgt catggagcgg ctcgagggcg
      301 aggacctcaa cgccgccctg cactcgcgcg gaccgctgag ccccgaggag gtcgtcgacg
      361 cgctcgcgca ggcgtgcatc gccgtggcgg ccgcgcacgc gtgcggcatc atccaccgcg
      421 acctcaagcc ggcgaacctg ttcatgacgc gccagccgaa cggcgccggg gtgctcaagg
      481 tgctcgactt cggggtctcg aagctcggcg aggagggcgc gccggagctg acacaggcga
      541 gcacgatggt cggctcggcg tattacatgt cgcccgagca gatgaacggc atgcgcgagc
      601 tcgacgggcg cagcgacatc tggtcgctcg gcgtgaccgc atacgagctg ttgaccggca
      661 agctgccctt cgatggctcg tcgatcgccg aggtgtgcgc gcaggtgctg tacgagccgc
      721 cgaagtcgcc gctgctgctg cgccccgatc tgccgcccgc cctgtgcgac gtggtcctgc
      781 gctgcctgag caaggaccgc gagcagcgcc acgcgaacgc cgacgagctc gccgaggccc
      841 tgctcggcag cctgccggcg caagccacga cctcgccgtt cacgcgacgc tccgcgcgct
      901 gaggccccgc ggtggcggat gcgccggccc tcgcgcagcc gggcgggtgg cgccgccggt
      961 gccgggtcgc cgcggtggcc gcgatcgtat tgccgcgccc gcggcctctg ctacgtgcac
     1021 gctatgcgag cgccagacga caccgacccg atcgacccgc tggccccacg cgccgacgac
     1081 ccgctgatgt tcggccggcg ctggatgtgg atggcgcgcc tctacgaccg ccccgccgac
     1141 ggcaccacgc gcctgcgcac ctcggcggtg atcctcgtga tggtgctggt gctgatcgtc
     1201 gtctcgctcg gcgtcgtgct gctcaccggc tgacgcgacg atacgggcgg tactccggct
     1261 cccacacctg cgcggtgatc cggcgcgcga gctcgtccgg gctgcacggc tcggcgacac
     1321 cgtcggcctg ggcctgaagg ccgaccgcct gggcgacgac gacggcgacc cgccgcagct
     1381 cgctgaccgc gggcaacagc ggcgcggtcg ggtcggcgag cgcgggcgac aatgtggcga
     1441 gcgcgcgggc ggccgcggtg aacatcgcgt cggtgacccg acgcgccccg ctggcgagga
     1501 tcccgaggcc gacgcccggg aacacgtacg agttgttggt ctgggcgatc gccacctcgc
     1561 ggccgcccca cgggaccggc gcgaacgggc tcccggtgcc gatcagcgcg cggccgtcgg
     1621 tccagcgcat caggtcgtcg ggcacggcct cggcgcgcga ggtcgggttc gacagcggaa
     1681 agatgacggg ccgctcgacg ctcgccgcca tcgcccggat cgccgcctcg gagaacgcgc
     1741 ccggctgccc cgacacgccg atcaatacgg tcggtcgcgc gttgcgggcg acgtcgacga
     1801 ggccgatgaa gtcggggttc tcgcgcgtcc acccggcgac tgcctggcgg cgctgcacga
     1861 agggctcctg cgcgggccgg atcccgggca tgccctcgac gagcaggccg tcgcggtcga
     1921 gcgcgtagaa gcgctcgcgc gcggcggcct cggacagacc ggccgcgatc atcgcgcgca
     1981 gcagcagcgc cgcgatccca cacccggccg agccggcgcc gacgatcgcg atccgctgct
     2041 cggtcaacgg cacgcgggtg accgccatcg ccgacagcag ggtcccggcg gcgaccgccg
     2101 cggtgccctg gatgtcgtcg ttgaacgagc acaggcggtc gcggtaagcg tcgagcaggc
     2161 ggccggcgtt ctggttggcg aagtcctccc attgcagcag ggcgtcgggc cagcgctgct
     2221 gcacggcgac gatgaagcgc tcgacgaaat cgtcgtactc cgcgccgcgg atccgccggc
     2281 tgcgccagcc gacgtacatc gggtcgttga ggcggtcggg gttatcggtg ccgacgtcga
     2341 ggaagattgg gagcgtgcgc tccgggtgga tgccggcgca cgcggtgtac agcgagagct
     2401 tgccgatcgg gatccccatg ccgccggtgc cctgatcccc gaggccgagg atccgctcgc
     2461 cgtcgctgac gacgatcacg cgcacgcgat cgtagcgcgg actggcgaac atgtcggcga
     2521 tccggtggcg gttgggatag ctgaggaaca gaccccgcgg gcgccgccag atctcgctga
     2581 agcgctggca gccctcgccg accgtcggcg tgtacacgat cggcagcagc tccggcgcgt
     2641 agcggaccag cagcgcgtag aacagggtct cgttgacgtc ctgtagatcg cgcaggaacg
     2701 cgtaacgctc gagcgggttg tcgcaggcgc gcagcgcctt gagccgccgc gcgacctg
//
LOCUS       DASWNJ010002025         3078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_633711, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002025 DASWNJ010000000
VERSION     DASWNJ010002025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633711"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..1037
                     /locus_tag="VGB85_33765"
     CDS             201..1037
                     /locus_tag="VGB85_33765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY0139120.1"
                     /translation="MTALVTGFVHSVTAQPSVQYRRNKWFYFLYDGAYLAGLVGLNVY
                     LIATHAQPLVGAWSWTYLALLPLVIYAHILLNVFIHNCCHGNFPRPINRLIGELAGLV
                     VITRYASWEIIHQRHHRHSDDPEKDPHHVLPNFARFLYETMLFNVERQLQNQAYDQFG
                     DTPENRKRERVRAVLSFATMLPLLLAWYLLLGPYAFFFLFVPAQAVGWVQVAHFNWAT
                     HNAHSPTGDYKPVNLDHSLYWLGNRIWFGLYMHGNHHKRANIFNPAKMDEVMARRAAM
                     RS"
     gene            1135..2469
                     /locus_tag="VGB85_33770"
     CDS             1135..2469
                     /locus_tag="VGB85_33770"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0139121.1"
                     /translation="MERPLYYVTALSNFARGYDKYARSYDKARIPESSFPERFFLLTR
                     DELPIGINKAAHLPQKTGLPGDRLIALETHATAEELQPNLRTGLGRFVPRGQIDVDGV
                     HLISEVGELLPIAIEEACARSLKMIVGELPPFAAMTPRSVSLLPIARACPARCPFCFS
                     KASVSDDMITAPLDWHRIDAVLGAARARGATRAVITGGGEPTLMPRLELLCMVEMCAR
                     DFTTVVLISNGFKWGRMDEAARAMALRELADTGLTVLSLSRHHHDDAANAALMGLDTG
                     SEKVAATWSRMHDTGLRMRWVCVLQRGGVEDPESLARYLDWAVDTGVGELCFKELYVS
                     TSIESEYHDHAANVWSAAHQVPLRLIVEFARAQGWPVATTLPWGAPVFAATWNGRPLK
                     IAAYTEPSVLWELTNGLCRSWNLMADGRCLASLEDHRSEVLPRGLRSLSIVS"
     gene            2438..>3078
                     /locus_tag="VGB85_33775"
     CDS             2438..>3078
                     /locus_tag="VGB85_33775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139122.1"
                     /translation="MDYAAFRSFRDLTVREREVLRADCMNPAKALARWRHAPADLAVD
                     TDATRALAAWTAASGLAIAHDRMVAGEGVRDLLRALLDIIDITELWLPEDVYPVYWQL
                     ASSTTPRPFATLPDLDLGFLADAGAHAVVVLPAPVTPLGRALRPDELATLHAWLLASP
                     ARRLVIDAVYTYDFAASRDMVETLLATDQCVLLWSCTKSWLCPDGLGIAAGPRS"
BASE COUNT          454 a         1190 c          933 g          501 t
ORIGIN      
        1 cgccgctcgt cacgcgcctg cgcaagcccc tgcgcaccgt ccgtcaccat cgagctcatc
       61 gcgccgggac catacaccgg cccgcctgca ccctcaacgc gctaaccatg cgcactttcg
      121 cgaccgggca gaatctcgac gctgcgccgg accccgtgtt attcgtgccc gcaacggccc
      181 ctggccgccg tgaggtcgcc ttgactgccc ttgtcactgg attcgtacac agcgtcaccg
      241 cccagccctc ggtgcaatac cgcaggaaca agtggttcta cttcctctac gacggcgcct
      301 acctggccgg gctcgtcggg ctaaacgtgt acctgatcgc tacgcacgcg cagccgctcg
      361 tcggcgcgtg gagctggacg tacctcgcct tgctgccgct ggtgatctac gcccacatcc
      421 tgctcaacgt cttcatccac aactgctgcc acggcaactt cccgcgcccg atcaaccgcc
      481 tgatcggcga gctcgccggc ctcgtggtca tcacgcgcta cgcctcctgg gagatcattc
      541 accagcggca ccatcgtcac tcggacgacc cggagaagga cccccaccac gtcctcccga
      601 acttcgcccg cttcctctac gagacgatgc tgttcaacgt cgagcgccag ctgcaaaacc
      661 aggcctacga ccagttcggc gacacccccg agaaccgcaa gcgcgagcgc gtccgggctg
      721 tgctcagctt cgccaccatg ctgccgctgc tgctcgcctg gtacctgctg ctcggcccgt
      781 acgccttctt cttcctgttc gtgccggccc aggccgtcgg ctgggtccag gtcgcgcact
      841 tcaactgggc gacccacaac gcccactcgc cgaccggcga ctacaagccc gtcaacctcg
      901 accacagcct gtactggctc ggcaaccgca tctggttcgg cctctacatg cacggcaacc
      961 accacaagcg cgccaacatc ttcaaccctg cgaagatgga cgaggtcatg gcgcgccgcg
     1021 ccgcgatgcg ctcctgacgt ctgcttactc gctggctcgc cctcgcgcgc acgcaattcg
     1081 atcgcggtca cgcgcctcga ctttcgccgg ggccgcgaac acactgcttg gcctgtggaa
     1141 cggccgctgt attacgtgac ggccctgtcg aacttcgccc gcggctacga caagtacgcc
     1201 cgcagctacg acaaggcgcg gatccccgag agcagctttc ccgagcgctt cttcctgctc
     1261 acgcgagacg aacttccgat cgggatcaac aaggccgccc acctgccgca aaagacaggt
     1321 ctgcccggcg accgcctgat cgccctcgag acccacgcca cggccgagga gctgcaaccc
     1381 aacctgcgca ccggcctcgg ccgcttcgtg ccgcgcgggc agatcgacgt cgacggtgtg
     1441 cacctgatct cggaggtcgg tgagctgctg ccgatcgcga tcgaggaggc ctgcgcgcgc
     1501 tcattaaaaa tgatcgtcgg cgagctgccg ccgttcgccg cgatgacgcc gcgctcggtg
     1561 tcgctgctgc cgatcgcccg cgcctgccca gcccgctgcc cgttctgttt ctcgaaggcc
     1621 tcggtgtcgg acgacatgat caccgcgccg ctcgactggc atcgcatcga cgcggtgctg
     1681 ggggccgcgc gggcccgcgg ggcgacgcgc gccgtgatca ccggcggcgg cgagccgacc
     1741 ctcatgcccc gcctcgagct gctgtgcatg gtcgagatgt gcgcccgtga cttcaccacc
     1801 gtcgtcctga tctccaacgg cttcaagtgg gggcgcatgg acgaggccgc ccgcgccatg
     1861 gcgttgcgag agctcgcgga caccggcctg accgtgctct cgctgtcccg ccatcaccac
     1921 gacgacgccg ccaacgcggc cctgatgggc ctcgacaccg gcagcgagaa ggtcgccgcg
     1981 acctggtcac gcatgcacga caccggcctg cgcatgcgct gggtctgcgt gctgcagcgc
     2041 ggcggcgtcg aggatccgga gtcgctcgcg cgctacctcg actgggcggt cgacaccggc
     2101 gtcggcgagc tctgttttaa agagctctac gtctcgacct cgatcgagtc cgagtatcac
     2161 gaccacgccg ccaacgtgtg gagcgccgcg caccaggtcc cgctgcgcct gatcgtcgag
     2221 ttcgcgcggg cgcagggctg gccggtcgcg acgacgctgc cctggggcgc cccggtgttc
     2281 gcggcgacct ggaacggccg gccgctgaag atcgcggcct acaccgagcc cagcgtgctc
     2341 tgggagctga cgaacggcct ttgtcgcagc tggaacctga tggccgacgg gcgctgcctg
     2401 gcgtcgctcg aggatcatcg cagcgaggtg ctaccccgtg gactacgcag cctttcgatc
     2461 gtttcgtgat ctcaccgtcc gcgagcgcga ggtgctgcgc gccgactgca tgaaccccgc
     2521 gaaggcgctg gcccgctggc gtcacgcgcc cgcggatctc gctgtcgaca ccgacgcgac
     2581 ccgcgccctc gcggcctgga ccgcggcgag cggcctggcg atcgcgcacg accgcatggt
     2641 cgccggcgag ggcgtgcgcg acctgctgcg cgccctcctc gatatcatcg acatcaccga
     2701 gctgtggctg cccgaggacg tctatcccgt gtactggcag ctggcgtcat cgaccacccc
     2761 gcgccccttc gccaccctgc ccgacctcga cctcggcttc ctcgcggatg ccggcgcgca
     2821 cgccgtcgtc gtgctgcccg ccccggtcac tccgctcggc cgcgcgctgc gacccgacga
     2881 gctcgcgacc ctgcacgcct ggctgctcgc atcgcccgcg cgccgcctcg tgatcgacgc
     2941 cgtctatacc tacgacttcg ccgcgagccg cgacatggtc gagaccctgc tcgccaccga
     3001 ccagtgcgtg ctgctgtggt cgtgcaccaa gtcatggctg tgcccggacg gcctcggcat
     3061 cgccgccggt ccccgatc
//
LOCUS       DASWNJ010002026         2019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_421107, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002026 DASWNJ010000000
VERSION     DASWNJ010002026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421107"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="VGB85_33780"
     CDS             <1..740
                     /locus_tag="VGB85_33780"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEY0139123.1"
                     /translation="LASVLAFSGALLANPQPAVAAVSGSEVLAKLDGLAARFPSQTYK
                     ATMDIYKSGKLRTTMVFSMSMKNLEKQFIHFTAPGDVAGMKILMEDRDTIYMYSPEFQ
                     KVRRVAAHLQNQGFLGSEFTPEDMVMAKLSPSFDATVVGKQGTETQLDLKPKAGSSIS
                     FSKLEVFIDSTVNGVTRIKYYDGSGNHVRTQTREEWKPFEDIKIPTRIRMKNLKTGDE
                     TVINMAEIDVKTPIGDDLFSRRTLMRG"
     gene            750..>2019
                     /locus_tag="VGB85_33785"
     CDS             750..>2019
                     /locus_tag="VGB85_33785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139124.1"
                     /translation="MVWTCAALLAPLALWAPAPAQRDYVGPGIVQASPAPTSTTPTTP
                     VAEAPAPTQPGPSASGVSPPGAASPGAAAPTGTTPAKPPPPSRPADLSMDDVFGGPAE
                     GPAELPSGPNPTGSDINAGVGANKSPGELGMDDVFGGGTPATPSGAAGPNKSTREASI
                     PTISPYKGLIDPENPGADKGGGAPTSHYGDKLKTRFRLVSSVYGDVDRANPRRLSRNE
                     NRLELYVAYTPNKHVQVVADAEPVLMGVSQARELDDLASQVYIQRFHMESDAAYVALT
                     DLAPGLDIKLGRQIVVWGTADKFNPTNNINADDVEDRPLFTEPIANQMAVVDYAPLGD
                     RLYLQGVYVPMFFPALLPPSAAYGLKDPFAEVPFSNQVQKDKINYLQETFQPANPKFT
                     PNVFGHVVPPENAFKNGQAAFKLGSRLGEVD"
BASE COUNT          362 a          766 c          615 g          276 t
ORIGIN      
        1 tcctcgcctc ggtcctcgcc ttctccggcg ccctgctcgc caacccccag ccggccgtgg
       61 cggccgtcag cggctcggag gtcctcgcca agctggacgg cctcgcggcc cgcttcccga
      121 gccagaccta caaggcgacg atggacatct acaagagcgg caagctgcgg accacgatgg
      181 tctttagcat gtcgatgaag aacctcgaga agcagttcat ccacttcacc gcccccggtg
      241 atgtcgccgg catgaagatc ctgatggagg accgggacac gatctacatg tacagcccgg
      301 agttccagaa ggtccgccgc gtcgcggccc acctgcagaa ccagggcttc ctcggcagcg
      361 agttcacccc cgaggacatg gtgatggcca agctgtcccc gagcttcgac gccacggtcg
      421 tcggcaagca gggcaccgag acccagctcg acctcaagcc caaggccggc tcctcgatct
      481 ccttctccaa gctcgaggtc ttcatcgact cgacggtcaa cggtgtgacc cggatcaagt
      541 actacgacgg ctccggcaac cacgtgcgca cccagacccg cgaggagtgg aagcccttcg
      601 aggacatcaa gatcccgacc cggatccgga tgaagaacct caagaccggc gacgagacgg
      661 tcatcaacat ggcggagatc gacgtgaaga ccccgatcgg cgacgatctc ttctcgcgtc
      721 gcacgctgat gcgcgggtaa actccgggcg tggtctggac ctgcgccgca ctcctcgccc
      781 cgctcgccct gtgggccccc gcgcccgcac agcgtgatta cgtcggcccc ggcatcgtcc
      841 aggcgagccc ggccccgacg agcaccacac cgacgacacc cgtcgccgaa gcacccgcgc
      901 cgacacagcc gggtccttcg gcttccggcg tttcgccgcc cggcgccgcg tcccccggcg
      961 ccgcggcccc gaccggcacg acgcccgcca agccgccccc gccgagcagg cccgccgacc
     1021 tgtcgatgga cgacgtgttc ggcggcccgg ccgagggccc cgccgaattg ccctcgggcc
     1081 cgaacccgac cggcagcgac atcaacgcgg gcgtcggcgc caacaagtcg cccggcgagc
     1141 tcggcatgga cgacgtgttc ggcggcggca cgccggcgac accctcgggg gccgccgggc
     1201 cgaacaagtc gacgcgcgag gcctcgatcc cgacgatctc gccgtacaag ggcctcatcg
     1261 accccgagaa ccccggcgcc gacaagggcg gcggggcccc gacctcgcac tacggcgaca
     1321 agctcaagac ccgctttcgc ctcgtcagca gcgtctacgg cgacgtcgac cgcgcgaacc
     1381 cgcggcgcct gagccgtaac gagaaccgcc tcgagctcta cgtcgcgtac acgcccaaca
     1441 agcacgtgca ggtggtcgcc gacgccgagc cggtgctgat gggcgtgtcg caggcgcgcg
     1501 agctcgacga cctcgcgtcg caggtctaca tccagcgctt tcacatggag agcgacgcgg
     1561 cctacgtcgc gctcaccgac ctcgcgcccg gcctcgacat caagctcggc cgccagatcg
     1621 tcgtgtgggg cacggcggac aagttcaacc cgaccaacaa catcaacgcc gacgacgtcg
     1681 aggaccgccc gctgttcacc gagccgatcg ccaaccagat ggcggtcgtc gactacgcgc
     1741 cgctcggcga tcgcctgtat ctgcagggcg tctacgtgcc gatgttcttc ccggccctgc
     1801 tgccgccctc ggccgcgtac ggcctcaagg acccgttcgc cgaggtgccg tttagtaatc
     1861 aggtccaaaa ggacaagatc aactacctgc aggagacctt ccagccggcc aaccccaagt
     1921 tcacgccgaa cgtcttcggc cacgtcgtgc cgccggagaa cgcttttaaa aatgggcagg
     1981 cggcgttcaa gctcggctcg cggctcggcg aggtcgacc
//
LOCUS       DASWNJ010002027         1653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_421152, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002027 DASWNJ010000000
VERSION     DASWNJ010002027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421152"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..1009
                     /locus_tag="VGB85_33790"
     CDS             221..1009
                     /locus_tag="VGB85_33790"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HEY0139125.1"
                     /translation="MSPLAVAVVACAALAALCWSLSLLTREYSWVDRVWSLAPVGYVA
                     WFAAQTGFTDPRLVLMTVLVAAWGARLTFNFARKGGYARGGEDYRWAVLRGMMSPALF
                     QAFNLVFIAAYQNALLLLISLPAWAALREPPAPLGALDLAATVAFVLLLIGETVADQQ
                     QWDFQRAKARGQATGFVTTGLFRCSRHPNFFCEQGQWWCFYLFSIAAGAGAINLSLVG
                     PVLLTLLFLGSTSFTESISVARYPAYREYQRRTSRLIPWLPGRA"
     gene            1118..>1653
                     /locus_tag="VGB85_33795"
     CDS             1118..>1653
                     /locus_tag="VGB85_33795"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0139126.1"
                     /translation="MTARARGDLAAAEAAGQVVVRGTPDDPRGWVLLGSLAHLEGSLA
                     RAAALYRRALASAPADPCAHAALAVALVQTGQPITAQAHFAAATGAGLERWSELRVRR
                     QAHALATMPAGASPRAWDACTTPLFEPADPCHMSLGTCTTISGRVAWTVERLAAALRE
                     RPRVVALTGAGISAASGL"
BASE COUNT          177 a          596 c          607 g          273 t
ORIGIN      
        1 ggggcgcacc tcgggggcgc gccgggctgg gctcaaagcg accgcacgca ccgctgtccg
       61 cacggcgaga tgctgcacct gctcgagtac gacggtaatc agttcctcga cggcgcgctc
      121 cacgtgttct gctgccgcga gcagacctgc gacctcgcct acgcggccga gttctgacgg
      181 acgccttctt tcacggacgc gagacgtata ggctccccgc atgtctccgc tcgccgtcgc
      241 cgtcgtcgcc tgtgccgccc tcgcggcgct gtgctggtcg ctctcgctgc tgacccgcga
      301 gtactcgtgg gtcgaccgcg tgtggtcgct cgccccggtc ggttatgtcg catggttcgc
      361 ggcgcagacg ggcttcacgg atccgcggct ggtgttgatg acggtcctcg tcgctgcgtg
      421 gggcgcccgc ttgaccttca acttcgcgcg caagggcggc tatgcccgcg gcggcgagga
      481 ttatcgctgg gccgtgctgc gcggcatgat gtcaccggcg ctgttccagg cgttcaacct
      541 cgtgttcatc gccgcctacc agaacgcgct gctgctgctg atctcgctgc cggcctgggc
      601 cgcgctgcgc gagccaccgg cgccgctggg cgccctcgac ctcgcggcga cggtcgcgtt
      661 cgtgctgctg ctcatcgggg agaccgtcgc cgaccagcag cagtgggact tccagcgggc
      721 caaggcccgc gggcaggcga ccggcttcgt gacgaccggc ctgttccgct gctcgcggca
      781 cccgaacttc ttctgcgagc agggccagtg gtggtgtttt tatctgttct cgatcgcggc
      841 gggcgcgggc gcgatcaacc tctcgctcgt cggtccggtg ctgttgacgc tgctgttcct
      901 cgggtcgacg agcttcaccg agagcatctc ggtcgcccgc tatccggcct atcgcgagta
      961 tcagcgccgg acctcgcggc tgatcccctg gctgcccggg cgcgcctgag cgccgtcgcg
     1021 gactcgcgcg ctcgcggccc ttccctgggg cccacggcgg agcgacactg cggccctgga
     1081 cgcgaacctc gagagtgatg ccgcgctcgc cctcggcttg acggcccgcg cgcgcggaga
     1141 cctggccgcg gccgaggctg cagggcaggt ggtggtgcgc ggcacgccgg acgacccgcg
     1201 cgggtgggtg ctgctcggct cgctcgcgca tctcgagggg tcgctcgcgc gggcggccgc
     1261 gctgtaccga cgcgcgctcg cctcggcgcc ggccgacccg tgtgcccatg ccgctctggc
     1321 ggtcgcgctg gtccagaccg gccagccgat caccgcccag gcccacttcg cggcggcgac
     1381 cggcgccggc ctcgagcgct ggagcgagct gcgggtccgg cgacaggcgc atgcgctggc
     1441 gacgatgccc gcgggcgcct cgccgcgggc gtgggacgcg tgcacgaccc cgctgttcga
     1501 gccggcggat ccatgccaca tgtccctcgg gacatgtacg acgatatcgg gacgggtggc
     1561 gtggacggtg gagcgactgg cggcggcgct gcgcgagcgg ccgcgggtgg tcgccttgac
     1621 cggcgcgggc atctcggcgg cgagcgggct gga
//
LOCUS       DASWNJ010002028         1763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_634075, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002028 DASWNJ010000000
VERSION     DASWNJ010002028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634075"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="VGB85_33800"
     CDS             <1..1152
                     /locus_tag="VGB85_33800"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0139127.1"
                     /translation="DPILVRRFAQEGQLAAQIRHPNLVPAYDLGWVGGRRYIVLELVD
                     GTSLNARLKDGPLPWARSVPIVLDVLAGLAALHARGVTHRDISPNNCMIEVRDGTERA
                     YLLDLGYARVIEDRGLVLTPPDASVSKIIYGSEGFIAPERLHGRPGDYRADVFSVGAL
                     WYAMLTGTALPDPMDAEPCSALDKMPLPPRLQAVLRGALDVRDRRHHSAASMAEALRV
                     AMRNIAAPPPWKKRAWWLAPGLGAVILPTWLVVRSGPPMIDCPRVSTPVQPLAAPVAR
                     PLPAEPAASGVLQHEAPGEPEPAAQVAEEARITGASDNKRVTAPRRRFDLSAALAKCK
                     PHPTTRLEVKYDPAGPLKINDDPPQGEMGRCVADVLQRHPPRRAVTLTP"
BASE COUNT          265 a          611 c          606 g          281 t
ORIGIN      
        1 gacccgatcc tggtgcgccg cttcgcgcag gaagggcagc tcgccgcaca gatccgacac
       61 ccgaacctcg tccccgccta cgatctcggc tgggtcggcg gccgccgcta catcgtgctc
      121 gagcttgtcg acggtacgag cctcaatgcg cgcctcaagg atggcccgct gccgtgggcg
      181 cggagcgtcc cgatcgtgct cgacgtgctc gccgggctcg ccgccctgca cgctcgcggt
      241 gtgactcacc gcgacatctc gccgaacaac tgcatgatcg aggtccgcga tggcaccgag
      301 cgcgcgtact tgctggatct cgggtacgcg cgcgtgatcg aggaccgcgg gctggtgctg
      361 acgccccccg atgcgtccgt gtcgaagatc atctacgggt cagagggctt catcgcgccg
      421 gagcgcctac acggccgtcc cggcgactac cgggccgacg tcttctcggt tggtgcgctc
      481 tggtacgcga tgcttaccgg gacggcgctg cccgatccga tggacgccga gccgtgcagc
      541 gcgctcgaca agatgccgtt gccgccgcgc ctgcaagccg tgctgcgcgg cgcgctcgat
      601 gttcgcgatc ggcgtcacca cagcgcggcc tccatggcgg aggcgcttcg tgtagcgatg
      661 cgcaacatcg cagctccgcc gccatggaag aaacgcgcct ggtggctcgc acctgggctc
      721 ggtgcagtaa tcttgccgac gtggctggtg gtgcgctccg ggccgccgat gatcgattgc
      781 ccgcgcgtat cgacgccggt gcaaccgctt gccgcgcccg tcgccaggcc gctccctgcc
      841 gagccggcgg cgtcgggcgt gttgcaacac gaggccccgg gcgagccaga gcccgccgcc
      901 caggtcgctg aggaggcccg catcaccggc gcgtcggaca acaagcgggt gacggcgccc
      961 cggcggcgct ttgatctcag cgccgcgctg gcgaagtgca agccgcaccc gaccacgcgc
     1021 ctcgaggtca agtacgaccc ggccggcccg ctgaagatca acgacgatcc tccccaggga
     1081 gagatggggc gctgcgtcgc ggacgtgttg caacgccacc caccgcgtcg agctgtcacg
     1141 ctgacgccgt agcgggcacg gttccaattg ttgcgatcgt ttgtaactgc cgattccttg
     1201 ccatctcttt acctttcgag ctggcgacgt cggctgacga gggcgggcac aaagatccca
     1261 gagattcgat ggatccgaga gatccgagag atcgaaactc gggctcgcac gcgatgagcc
     1321 gcgccgaggc ttggcgcaaa gtcggacccg cgcggcgata cgcgtcccgc agcccgcgct
     1381 cgggcgtcgg tcgcagctcc gcctgcaggc cgcgcagcag ggcgtgcgga ccgcgggccg
     1441 gccaactact ccaccggctc tccggcgctt cgttgctgtg ctccaggatc gaccgggcac
     1501 ggcttgatgg cgcccctgcg acagccgacc gagttgtgcg cgagccgccg ccgagttggc
     1561 tatcaggcag gcgcgactgc acgtcgagtg catgcagatc gtgctcgtct gcatggcgag
     1621 agagcgcgga cttgtgctca tgttcgtgtc gatgtggtgc agagttcccg tcaggatcga
     1681 ccgactgccg accaagccgc gctggagcgc gcggagagtg tgcgtcgagt cggcgccgag
     1741 taggtcacga tacgggcccg ata
//
LOCUS       DASWNJ010002029         1769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_634314, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002029 DASWNJ010000000
VERSION     DASWNJ010002029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634314"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..890
                     /locus_tag="VGB85_33805"
     CDS             186..890
                     /locus_tag="VGB85_33805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139128.1"
                     /translation="MRKINLMNEAARNAQVVIESVRKQDGPTQGLPGKKLAFKRYVAT
                     TENGLHSKLAQNHGDALAKALIEGDPEIDKEVVGRDVGDTQTVFLSGTGEVLHASPSL
                     VEVIFGADGKERERKPAESVPANTNEESPVRWTKMRLKRSEVVTKFAFCRTIQIKHVD
                     GLSFDFLHAMAKELADKDEVARLGAGPKGRDPLIFQENGARYQAFLEGRVDGPRYKLL
                     LHLSNLELRTIAGAAS"
     gene            912..>1769
                     /locus_tag="VGB85_33810"
     CDS             912..>1769
                     /locus_tag="VGB85_33810"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139129.1"
                     /translation="MTLYSLDSARPLGKGRHSPAGSVTDLELAAAVRNYKRVVAGSYR
                     AMVQDDLATALPHGNLLVSPKIDGELWYLVFDEGDVFLSSPTGRVVSGDVPVLAEARA
                     IAGKLKGRTVFAGELFAVKPAGQGRARVGDLANIMGGEDKAQVERMVYHAFDLVQGGD
                     AETPVRSIVYAERLAVLQRVLAGGKRLQAIKTEAVTGSERVLALWNDWVEGGKGEGLV
                     IRAEGLVIKAKPSITIDAVVIGYTDSSDDPEGVGALLMALMRDDGTFQVIGSCGNMPT
                     EERHRFKKAL"
BASE COUNT          303 a          595 c          627 g          244 t
ORIGIN      
        1 cggcggctac cagagcgaga ccgcgttcct ggtcggcaac gccagcggct tcttcgcgct
       61 cgtcggcacg ccgaccgaga cggcgtggct cgagctggcg acggtcgcca ccgagacgag
      121 cgacgacgcc ggcgacggtg acgacgacga gctggacttc gagatgttct gaccgaggag
      181 ccgccatgcg taagataaat ctgatgaacg aggccgcgcg taacgcccag gtcgtgatcg
      241 agtccgtgcg caagcaggac ggtccgaccc aggggttgcc gggcaagaag ctggccttca
      301 agcgctacgt cgcgaccacc gagaacggcc tgcatagcaa gctggcgcag aaccacggcg
      361 acgcgctggc gaaggcgctg atcgagggcg acccggagat cgacaaggag gtcgtcggcc
      421 gcgacgtcgg cgacacgcag accgtgttcc tgtcgggcac cggcgaggtg ttgcacgcct
      481 cgccctcgct ggtcgaggtg atcttcggcg ccgacggcaa ggagcgcgag cgcaagccgg
      541 ccgagtcggt gcccgcgaac accaacgagg agtcgccggt gcgctggacc aagatgcgac
      601 tcaagcgcag cgaggtcgtc accaagttcg cgttttgtcg cacgatccag atcaagcacg
      661 tcgatggcct gagcttcgac ttcctgcacg cgatggcgaa ggagctcgcc gacaaggacg
      721 aggtcgcccg cctcggcgcc ggacccaagg gccgcgaccc gctgatcttc caggagaacg
      781 gcgcgcgcta ccaggccttc ctcgagggcc gcgtcgacgg gccgcgttac aagctgctgc
      841 tccacctcag caacctcgag ctgcgcacga tcgccggcgc cgcgagctga tcggacaagg
      901 gaccctctca catgacactc tactccctcg atagcgcccg tccgctcggc aagggccgtc
      961 actcgcccgc cggctccgtc acggatcttg agctcgccgc ggccgtccgc aactacaagc
     1021 gcgtcgtcgc cggcagctac cgcgcgatgg tccaggacga cctcgctact gccctgccgc
     1081 acggcaacct gctcgtctcg ccgaagatcg acggggagct gtggtacctc gtgttcgacg
     1141 agggcgacgt gttcctcagc tcgccgaccg ggcgcgtggt gtccggcgac gtgccggtgc
     1201 tcgccgaggc gcgggcgatc gcgggcaagc tcaagggccg caccgtgttc gcgggcgagc
     1261 tgttcgcggt caagcccgcg ggccagggcc gggcgcgcgt cggcgacctg gcgaacatca
     1321 tgggcggcga ggacaaggcg caggtcgagc gcatggtcta tcacgcgttc gacctcgtgc
     1381 agggcggcga cgccgagacg ccggtgcgca gcatcgtcta cgccgagcgc ctcgcggtgc
     1441 tgcagcgggt gctcgccggc ggcaagcgcc tgcaggcgat caagaccgag gcggtgaccg
     1501 gcagcgagcg cgtgctggcg ctgtggaacg actgggtcga gggcggcaag ggcgagggcc
     1561 tggtgatccg cgccgagggc ctggtgatca aggccaagcc gtcgatcacg atcgacgcgg
     1621 tcgtgatcgg ctacaccgac agctccgacg accccgaggg cgtcggcgcc ttgctgatgg
     1681 cgctgatgcg cgacgacggc acgttccagg tcattggttc atgcggcaac atgccgaccg
     1741 aggagcgcca ccgcttcaaa aaggcgctc
//
LOCUS       DASWNJ010002030         1790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_350111, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002030 DASWNJ010000000
VERSION     DASWNJ010002030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350111"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /gene="tilS"
                     /locus_tag="VGB85_33815"
     CDS             complement(<1..585)
                     /gene="tilS"
                     /locus_tag="VGB85_33815"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEY0139130.1"
                     /translation="MPPRHPSIAAALGHLRPAIAGVRSARGVPPDGRLALLVACSGGA
                     DSTALLGLLALITDADRLTLTVGHVDHGLRAGSAAEAEHVRTVAQSLGVAVHATRLTL
                     APGPGLPARARDARRAALRGMAAGAPVVLAHTQTDQAETLLMHATRGAGLEGVAAMPA
                     WDDPWCRPLLGVPREVTRALCDRLGLGFVDDPSNL"
     gene            647..>1790
                     /locus_tag="VGB85_33820"
     CDS             647..>1790
                     /locus_tag="VGB85_33820"
                     /inference="COORDINATES: protein motif:HMM:NF039677.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6178 family protein"
                     /protein_id="HEY0139131.1"
                     /translation="MTATDELGRLRPDDLRALQNDPLALEQRDDLPEFVPALPATTFV
                     AVARSLHAEGRLELVLPHATAEQITALFDLDAWRRDRVDIDRARLWLTEIVDLYAQTR
                     ERGELGELMMAMDPELWTFALLPGTSVFHLDPDDDEQRAQARDAVEALYVYETPDGYY
                     LVAVPDTETGHRAIHVLDRVYSDDLETGKALVDSLRGALGSMIEEDLLRWRAGRLADL
                     GFVPWEEAMKLLRPLDLKTARETSTGPTSVPTSDPASATLPAETPRSGLLRRALATLP
                     AAQQGERAREFTLLVNELMAAQRFEPGDLKLQERTFHQAEATVGLGLELLVAGITDPG
                     EQDSFLAGRITAIGLRGAFRAGYGPLDKLRRAALALHRDGHVSLSRI"
BASE COUNT          237 a          725 c          617 g          211 t
ORIGIN      
        1 gaggttgctc gggtcgtcga cgaagccgag gccgagccga tcgcacagcg cacgtgtcac
       61 ctcgcgcggc accccgagca gcggccggca ccacgggtcg tcccacgccg gcatcgccgc
      121 gaccccctcg agcccggcgc cgcgggtcgc gtgcatcagc agcgtctccg cctgatcggt
      181 ctgcgtgtgc gccagcacga ccggcgcgcc cgcggccatc cctcgcagcg ccgcgcggcg
      241 agcgtcgcgg gcgcgcgccg gcagccccgg gcccggcgcc agcgtgagcc gcgtcgcatg
      301 caccgccacg ccgaggctct gcgcgaccgt ccgcacgtgt tcggcctccg ccgccgaccc
      361 ggcccgcagc ccgtggtcga cgtgcccgac cgtcaatgtc agccgatcgg cgtcggtgat
      421 caacgccagc aggccgagca gcgccgtcga gtcggccccg cccgagcagg cgaccagcag
      481 cgccaggcgc ccgtccggcg gaacgccccg ggccgaacgc acgcccgcga tcgccgggcg
      541 caggtgaccg agcgccgcgg cgatgctggg atgacggggt ggcatgacga ccgtgaggag
      601 agcgacgcga ggtggcgcgc gcgcgcggcg atgatacctt gcccgcgtga ctgccaccga
      661 tgagctcggc cgcctccgtc ccgacgacct gcgcgccctc cagaacgacc cgctggcgct
      721 cgagcagcgc gacgacctgc cggagttcgt gcccgcgctg ccggcgacga ccttcgtcgc
      781 ggtcgcccgc agcctgcacg cggagggtcg cctcgagctc gtgctgccgc acgcgaccgc
      841 cgagcagatc accgcgctgt tcgacctcga cgcctggcgc cgcgaccgcg tcgacatcga
      901 ccgcgcccgc ctgtggttga cggagatcgt cgacctctac gcccagaccc gcgagcgcgg
      961 cgagctcggc gagctgatga tggcgatgga ccccgagctg tggaccttcg cgctgctgcc
     1021 cggcacctcc gtgttccacc tcgacccgga cgacgacgag cagcgcgccc aggcccgcga
     1081 cgccgtcgag gcgctctacg tctacgagac cccggacggc tattacctgg tcgccgtgcc
     1141 cgacaccgag accggccacc gtgcgatcca cgtgctcgac cgcgtctact ccgacgacct
     1201 cgagaccggc aaggcgctcg tcgactcgct gcgcggcgcg ctcggctcga tgatcgagga
     1261 ggacctgctg cgctggcgcg ccggtcgtct cgccgacctc ggcttcgtgc cgtgggagga
     1321 ggcgatgaag ctgctgcgcc cgctcgacct caagaccgcc cgcgagacca gcaccggccc
     1381 cacgagcgtg cccacgagtg accccgcgtc cgccaccctg cccgccgaga ccccgcgctc
     1441 ggggctgctg cgccgcgccc tggcgaccct gcccgccgcg cagcagggcg agcgcgcgcg
     1501 cgagttcacg ctgctggtca acgagctgat ggccgcgcag cgcttcgagc ccggcgacct
     1561 caagctgcag gagcgcacgt tccaccaggc cgaggcgacc gtcggcctcg gcctcgagct
     1621 gctcgtcgcc ggcatcaccg accccggcga gcaggacagc ttcctcgccg gccggatcac
     1681 cgcgatcggg ctccgcggag cgtttcgcgc cggctacggt ccgctcgaca agctgcgccg
     1741 cgccgcgctc gccctgcacc gcgacggcca cgtgtcgctg agccgcatcg
//
LOCUS       DASWNJ010002031         1775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_634497, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002031 DASWNJ010000000
VERSION     DASWNJ010002031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634497"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1775)
                     /locus_tag="VGB85_33825"
     CDS             complement(<1..>1775)
                     /locus_tag="VGB85_33825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139132.1"
                     /translation="LPGGDAVRSTASLVVKLLEHIDDDPDAAAMSWRVFVLVGDLTRA
                     EHALTSGLGVGTGRVRAGGPPPLLGTEGGTAAGGSGVTNGPGGAAGAGAKDSPGQATG
                     ATDTRSGAAGSAAKQGPGAAAGADPKDSQRNAPRSGAKGGAGAAGAVPKDSKARSGKP
                     EAPPATPVPSDSPPGPGFEPSQVRVPVPSLWSARRRVDADSLPKLLLLARLRALGGRD
                     DQALEIALFALAEAYAQGLPGAEAMARAEGRRLLAAGEPWAALAVASAVPGLATADLT
                     HAAGAAIVLAQAACPGGCTDAESARDRVAARQVLGEPWLLAQEGRARELAFARDLPGA
                     PATGCPELGELLAPGARGPLSTALGQARDELTHRPRRATAETGPKDQASRPATASPET
                     GEALRRAVEADPTMACAGRLVAPLLYAGDHRVAATALAEQLIHAPQEVASGVLALQSE
                     LALGLGRRDQADQMLVAAAAASADPGAVWLRAARLGAYVDARHYQLLALRQGLMHVPA
                     AEAAPLRLALVVRSLRDANDAWAARESDVGREALMRGVESYLDNEAPARRWQAREDLA
                     NALAEHRWADLEAAILIRAALWPEPG"
BASE COUNT          194 a          692 c          696 g          193 t
ORIGIN      
        1 cccggctcgg gccacagggc ggcgcggatc aggatcgcgg cctcgaggtc ggcccagcgg
       61 tgctcggcga gcgcgttggc gaggtcctcg cgcgcctgcc agcggcgcgc cggggcctcg
      121 ttgtcgaggt agctctcgac cccgcgcatc aacgcctcac gaccgacgtc cgactcccgc
      181 gccgcccagg cgtcgttggc gtcgcgcagc gagcggacga ccagcgcgag gcgcaacggc
      241 gcggcctcgg cggcgggcac gtgcatgagc ccctgacgca gcgcgagcag ctgatagtgc
      301 cgcgcgtcga cgtaggcgcc gagcctggcg gcgcgcagcc acacggcgcc gggatcggcg
      361 gacgcggccg cggccgcgac gagcatctgg tcggcctggt cgcggcgacc gaggcccagc
      421 gcgagctcgg actgcagcgc gagcacaccg gaggcgacct cctgcggcgc gtggatcagc
      481 tgctcggcga gcgcggtcgc ggcgacgcgg tggtccccgg cgtacagcag cggcgcgacg
      541 agccggcccg cgcaggccat cgtcgggtcg gcctcgaccg cgcggcgcag cgcctcgccg
      601 gtctcggggc tggcggtcgc gggccggctc gcctggtcct tgggaccggt ctctgcggtc
      661 gctcgccggg ggcgatgcgt gagctcgtcg cgcgcctggc cgagcgcggt cgacagcggg
      721 ccacgggccc ccggcgcgag cagctcgccg agctcggggc agccggtcgc gggggcgccg
      781 gggaggtcgc gggcgaacgc cagctcgcgc gccctgccct cctgcgcgag cagccacggc
      841 tcgccgagca cctgccgcgc ggcgacgcgg tcgcgcgccg actcggcgtc ggtgcagccg
      901 cccggacagg ccgcctgcgc gagcacgatc gcggccccgg ccgcgtgggt caggtcggcg
      961 gtggcgagcc cgggcaccgc gctggcgacc gcgagcgcgg cccacggctc accggccgcg
     1021 agcagccgcc ggccctcggc gcgcgccatc gcctcggccc cgggcaggcc ctgcgcgtac
     1081 gcctcggcga gcgcgaacag ggcgatctcg agggcctggt cgtcgcgtcc gccgagcgca
     1141 cgcaagcggg ccagcagcaa caacttcggc aagctgtcgg cgtcgacgcg ccgccgcgcc
     1201 gaccacagcg agggcaccgg cacgcgcacc tgcgacggct cgaagccggg gcccggcggg
     1261 ctgtccgaag ggacaggcgt cgcggggggc gcttcgggct tgccggacct cgccttgctg
     1321 tccttgggga cagccccggc cgcaccggca ccacccttcg cgccagatcg tggcgcattg
     1381 cgctggctgt ccttggggtc agctccggcc gcggcacccg ggccctgctt cgcggccgat
     1441 cctgccgcac cgctccgggt gtccgtcgcg ccggttgcct gacccgggct gtccttcgcg
     1501 ccagccccgg cagcgccacc cgggccgttc gtgacgcccg aaccgccagc cgctgtgccg
     1561 ccctcggtcc cgagcagcgg cggcggtccg ccggcgcgca cgcggccagt gccgacgccg
     1621 aggcccgagg tcagcgcatg ttcggcgcgg gtcaggtcgc cgacgagcac gaacacccgc
     1681 cagctcatcg cggcggcgtc ggggtcgtcg tcgatgtgct cgagcagctt gacgacgagg
     1741 ctcgcggtcg agcggaccgc gtcgccgccg ggcag
//
LOCUS       DASWNJ010002032         2313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_350253, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002032 DASWNJ010000000
VERSION     DASWNJ010002032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350253"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2313)
                     /locus_tag="VGB85_33830"
     CDS             complement(<1..>2313)
                     /locus_tag="VGB85_33830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139133.1"
                     /translation="RDSLISGQDPYGEQTGTQRCPVQAFEVVYREARPGAGRRLTAWL
                     GRQSGAAEQVEALWREALFLPQAQTGSVELGWALPGYRVKLGEHAVWGAAPELGGPWL
                     VRTGVRMLSLGAALAAAGLEVDAMAGWIECPALRVTADEAGVVQDAAFDMLVAWLHDA
                     REHSHRLPTRSSPTGQVGRDSEGAAWVVVWPTQVDSVLMASGRAIDREGVAQRARTGR
                     DFLYVWKHQQRQVPPAMQARTHALWPSELAALQASVPELRPVPVRALGDSPQVERVDL
                     TALMQSSLAPVPVDLGAAAVATTQGGARVELALRAFVHRTPTATMGATALLAFGRRVA
                     QVRESARVLPGVTLVCELTGASIDGLRGETALLQAVADRCREAGERVMPMLLAQGMAH
                     TSPWEVPLVRTQAEALTGAAISLRYRSERGEDGPELAWDESPLLGLMIGRDATGTRTI
                     SLQQALERCRDVGGIVVEATGQAWPGWVSRAPEHVPWSLTTEGRALLERVIGKDVLWD
                     MPTLPEMHALPRPVAEQAGLLLAPDEVARLAEKTGEDRARAAVLGHLLVKRAHGHGST
                     ELDEAPLLRRFDPRALQSVRRVSLTQALAEVPRPGLLPPGGVSRGLAGAALLVTPGEA
                     RLLHAEGFAPASATTKTTKTTAVAPAPGHAKPVRRAGGAGVSLLVVPVATATAVGALR
                     VDGTGPAKVALWSGGLHLDDLQLPAPLDCVGGRLVLTRQGARVVGERLAGEIQGLCRI
                     VVAQAIDQQRLARPGGAQQVGLAEFLARCQA"
BASE COUNT          302 a         1057 c          695 g          259 t
ORIGIN      
        1 cgcctggcag cgcgcgagga actccgcgag cccgacctgc tgcgccccac ccggccgcgc
       61 cagccgctgt tgatcgatcg cctgcgcgac cacgatccgg cacagcccct ggatctcgcc
      121 cgcgagccgc tcgccgacca cccgcgcgcc ctgccgcgtc aacaccagtc gaccgccgac
      181 gcagtcgagc ggcgccggca gctgcaagtc gtcgaggtgc aggccgccgc tccacagcgc
      241 caccttcgcc ggtcccgtcc cgtcgacccg caacgccccg accgccgtcg ccgtcgccac
      301 cggcacgacc agcaacgaca cccccgcacc gcccgcgcgt cgcaccggct tcgcgtgacc
      361 gggcgccggt gccaccgccg tcgtcttcgt cgtcttcgtc gtcgcgctcg ccggcgcgaa
      421 gccctcggcg tgcagcagcc gcgcctcgcc cggcgtcacc agcaacgccg cccccgcgag
      481 cccccgcgac acgccgcctg gcggcagcag ccccggccgc ggcacctcgg cgagcgcctg
      541 cgtcagcgac acccggcgca ccgactgcag cgcgcgcggg tcgaagcggc gcagcagcgg
      601 cgcctcgtcg agctccgtcg acccatgccc gtgcgcccgc ttcaccaata aatgcccgag
      661 caccgccgcc cgcgcgcggt cctcacctgt cttttcagcc agccgcgcga cctcgtcggg
      721 cgcgagcagc agccccgcct gctccgccac cggtcgcggc agcgcgtgca tctccggcag
      781 cgtcggcatg tcccaaagga catctttgcc gatcacccgc tcgagcagcg cccgcccctc
      841 ggtcgtcagc gaccagggca cgtgctccgg cgcgcgcgac acccagcccg gccacgcctg
      901 cccggtcgcc tcgaccacga tcccgccgac atcgcggcag cgctcgagcg cctgctgcag
      961 cgagatcgtc cgcgtgccgg tcgcgtcgcg cccgatcatc aggccgagca gcggcgactc
     1021 gtcccacgcc agctcgggcc cgtcctcgcc gcgctcgctg cggtaacgca gcgagatcgc
     1081 cgcgcccgtc agcgcctccg cctgcgtgcg caccagcggc acctcccacg ggctcgtgtg
     1141 cgccatcccc tgcgccagca acatcggcat cacccgctcg cccgcctcgc ggcagcggtc
     1201 cgccaccgcc tgcaacagcg ccgtctcccc gcgcagcccg tcgatgctcg cccccgtcag
     1261 ctcgcacacc agggtcacac ccggcaacac ccgcgccgac tcgcgcacct gcgccacccg
     1321 ccgcccgaac gccagcagcg ccgtcgcccc catcgtcgcc gtcggcgtac ggtgcacgaa
     1381 ggcccgcagc gccagctcca cccgcgcccc gccctgcgtc gtcgccaccg ccgccgcccc
     1441 gaggtcgacc ggcaccggcg ccagggagct ctgcatcaac gccgtcaggt cgacccgctc
     1501 gacctgcggc gagtccccga gcgcgcgcac cggcaccggc cgcagctccg gcacgctcgc
     1561 ctgcagggcc gccagctccg acggccacag cgcgtgcgtg cgggcctgca tcgccggcgg
     1621 cacctggcgc tgctgatgct tccacacata caaaaaatcg cgccccgtgc gagcccgctg
     1681 cgccaccccc tcgcgatcga tcgcgcggcc cgaggccatc aatacgctgt cgacctgcgt
     1741 cggccacacc accacccacg ccgcgccctc gctgtcccgc ccgacctgtc ccgtcggact
     1801 gctgcgggtc ggcaggcgat ggctgtgctc gcgcgcgtcg tgcagccacg cgacgagcat
     1861 gtcgaaggcc gcgtcctgca cgacccccgc ctcgtccgcc gtcacgcgca gcgccgggca
     1921 ctcgatccac ccggccatcg catcgacctc gagcccggcc gccgccagcg ctgcgccgag
     1981 cgacagcatc cgcacccccg tgcgcaccag ccacggcccg ccgagctccg gcgcggcacc
     2041 ccacaccgcg tgctcgccga gcttcacgcg gtaacccggc agcgcccacc cgagctcgac
     2101 cgacccggtc tgcgcctgcg gcaggaacag cgcctcgcgc cacagcgctt cgacctgctc
     2161 cgccgccccg ctctggcgcc ccagccaggc cgtcaaccgc cgcccggccc ccggccgcgc
     2221 ctcgcggtac acgacctcga aggcctgcac cggacagcgc tgcgtcccgg tctgctcgcc
     2281 gtagggatcc tgcccgctga tcaaactgtc tct
//
LOCUS       DASWNJ010002033         2250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_350389, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002033 DASWNJ010000000
VERSION     DASWNJ010002033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350389"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(288..1718)
                     /locus_tag="VGB85_33835"
     CDS             complement(288..1718)
                     /locus_tag="VGB85_33835"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0139134.1"
                     /translation="MTELSRLELDEADLRAMVAAALDRVCEQLRALPGQPACVTEGGA
                     ELARALREGPPRRGSPLEAVLATLFDQALPVSFNAASPGYLAYVPGGGLLHAAVADLI
                     SNSINRYVGVWRGAPGLVQLESNVIAWLAEVVGLPAGAGGILTSGGSLANFSATVAAR
                     HRLGERFTDGTIYTSTQAHHSVVRAALLAGFPAAQVRAVAVDASQRVRVDLMAAAIAE
                     DRARGLRPCMIAGSAGTTNTGAVDDLEALAGLAAREQMWFHVDAAYGGCFALTQRGQQ
                     RLRGLGLADSITLDPHKGLFLPYGTGALLVRDPQALRRAHSLHADYLPPMQEDPQFFD
                     FCEMSPELSRDFRGLRLWLPIKLCGLDVFAAALDEKLDLAAHAAQVLRVTPEIEIVAE
                     PQLSLLAFRHVPAALRGDEAAIDVHNERLLSAINRRARVYLTPTQLDGRFVLRICVLS
                     FRTHQDRVDAGLEDIRAAIAELAQAG"
     gene            1840..2079
                     /locus_tag="VGB85_33840"
     CDS             1840..2079
                     /locus_tag="VGB85_33840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139135.1"
                     /translation="MKPGKKPMAITLPDLNLAEAERRLCLEALTRTGSLVEAAELLGT
                     NRHALRRLIAKHGITWWPYAQQQNGWKRPSRGAQA"
BASE COUNT          351 a          825 c          775 g          299 t
ORIGIN      
        1 agttgggcca ttgcccttgt ccttagagcc ggtcttcttg gcgggcttgc tggggccttc
       61 atggccgtgg gctctgcagc cacgcggtcg accagctcgc ctaggctgag ctcggggtag
      121 atctggctct cctcaaggag agggggttgc cgtaatggag gctggcctgg gccaggatgc
      181 tcttggtgat attcgtcgtg gtcatcaacc catcatcggg actaccccag gtttctcgag
      241 ccagacccag cgggctcgtg gatgcccacc cgcgaatagc agacgattca gcccgcctgc
      301 gcgagctcgg cgatcgcggc ccggatatcc tcgagcccgg cgtcgacgcg atcctgatgc
      361 gtgcgaaacg acagcacgca gatgcgcagc acgaagcgcc cgtcgagctg cgtcggcgtc
      421 agatacacgc gggcgcggcg attgatcgca ctcaacaacc tctcattatg tacatcgatc
      481 gccgcctcgt cgccgcgcaa tgccgccggc acgtggcgaa acgcgagcag cgacagctgc
      541 ggctcggcga cgatctcgat ctccggggtc acgcgcagca cctgcgccgc gtgggccgcg
      601 aggtcgagct tctcgtcgag cgcggcggcg aagacgtcga gcccgcagag tttaattggc
      661 agccacaggc gcagcccgcg aaagtcgcgc gacagctccg gcgacatctc gcaaaagtcg
      721 aagaattgcg ggtcctcctg catcggcggg agatagtcgg cgtgcaggct gtgcgcgcgg
      781 cgcagcgcct gcgggtcgcg gaccagcagc gcacctgtcc catagggcag gaacaaaccc
      841 ttgtgcggat cgagggtgat cgagtcggcg aggccgaggc cgcgcaggcg ctgctggccg
      901 cgctgcgtca gcgcgaagca gccgccgtac gccgcgtcga cgtggaacca catctgctcg
      961 cgcgcggcga gcccggcgag cgcctcgagg tcatcgaccg cgccggtgtt ggtggtcccc
     1021 gcgctgccgg cgatcatgca cggacgcagc ccacgcgccc ggtcctcggc gatcgccgcg
     1081 gccatcaggt cgacgcgcac gcgctggctt gcgtcgaccg ccacggcccg cacctgcgcc
     1141 gccgggaagc cggccagcag cgccgcgcgg accaccgagt gatgcgcctg cgtcgacgta
     1201 tagatcgtgc cgtcggtgaa gcgctcgccg aggcggtgcc tggcggccac ggtggcgctg
     1261 aagttcgcga gcgacccgcc gctggtcagg atccctcccg cccccgccgg caggccgacg
     1321 acctcggcga gccaggcgat cacgttgctc tcgagctgga ccagaccggg cgcgccccgc
     1381 cacacgccga cgtaacggtt gatcgagttg gagatcaggt cggcgaccgc ggcgtgcagc
     1441 aggccgccgc cgggcacgta cgccagataa cccgggctcg cggcgttgaa gctgaccggc
     1501 agcgcctggt cgaacagcgt cgccagcacc gcctccagcg gcgagccacg ccgcggcggc
     1561 ccctcacgca gcgcgcgggc cagctcggcg cccccctcgg tgacgcaggc cggctgcccg
     1621 ggcagcgcac gcagctgctc gcagacgcgg tccagcgccg ccgcgaccat cgcccgcagg
     1681 tccgcctcgt cgagctcgag ccgcgagagc tccgtcatgg gacccagtta tcccgacccc
     1741 gccgggccgc gacagacatg ggggtccgca gccgcgtgcg gaaacccccg ctcgaaagcg
     1801 cgccacgcat ccccgagcca ggctatacga tcgcctcgca tgaagcccgg caagaagccc
     1861 atggcgatca cgctgcccga cctcaacctg gcggaggcgg agcgacggct gtgcctggag
     1921 gcgctgacgc gcaccggctc gctcgtcgag gccgcggagc tgctcggtac caaccgccac
     1981 gccctgcgcc gcctgatcgc caagcacggc atcacctggt ggccctacgc gcagcaacaa
     2041 aacggctgga aacggccatc ccgcggcgcc caggcctgag gcggccgagg ccgtcccgga
     2101 acggctggcg gccgcgcggt acgtcgcggc cagcgtgata ctcgcggccc gcctggcacc
     2161 gcgccgctgc gcaaaacctc gtggaccgcc cggaggcttc ccgacccggc cgccctgcgc
     2221 tacgagaggg agccgtgtcg ctgttcccct
//
LOCUS       DASWNJ010002034         2187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_422095, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002034 DASWNJ010000000
VERSION     DASWNJ010002034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422095"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VGB85_33845"
     CDS             complement(<1..411)
                     /locus_tag="VGB85_33845"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEY0139136.1"
                     /translation="MPTIRGVIFDLDGTLVDSNDAHILAWVEALAEFGWDVTQEQVRP
                     LVGMGSDKLLPALAGVALDSNEGHPVSTRRQALMARFVPQLRPFPGAVELVEALRRRG
                     LRIALASSATRPDVDRLLAQIGLEGSFDAVVSAGE"
     gene            495..1370
                     /locus_tag="VGB85_33850"
     CDS             495..1370
                     /locus_tag="VGB85_33850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY0139137.1"
                     /translation="MRIGVDLGGTKIEGAVLDAAGKVALRRRIPTPAGDYGQIVARIA
                     EVVLRLERESGTRCTVGVGTPGSENPSTGLMRNCNATIVNGKPLRRDLEAAIGRPVRL
                     ENDANCFALSEAVDGAGAGAAVVFGVIVGTGVGGGVVVRGRLISGRNRIAGEWGHSPL
                     PWAGPNELPGPLCHCGRRGCIETFLSGPALARDGGAAPELLASSTDPLARAALDRYID
                     RLARALVPVIHLLDPDVIVLGGGLSAIDRLYRDVPPLWPMHLFSDAPPTTLLRPAKHG
                     DASGVRGAAWLWHEP"
     gene            complement(1468..>2187)
                     /locus_tag="VGB85_33855"
     CDS             complement(1468..>2187)
                     /locus_tag="VGB85_33855"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY0139138.1"
                     /translation="GAMQARRGAFARADGGTLFLDELDALPLDLQPRLLRALETRTVR
                     PVGGDSEQAIDVRVVAAGQRDLAGAVASGALRPDLYYRLSVVRVTLPPLRARREDIAP
                     IVRSLLQQRGLASEGVEGPALARLTAQPWPGNVRELRNAVDRALALSPGARSFSELRW
                     YVPGDMSEKSGLEVDTERPFAAAKQAIVDAFEHRYLRALLDRVDGNISAAARSSELDR
                     KHLRTLLRKHGLIPDPGVPEE"
BASE COUNT          290 a          784 c          827 g          286 t
ORIGIN      
        1 ctcgccggcc gagacgaccg cgtcgaagct gccttcgagc ccgatctgcg cgagcaggcg
       61 gtcgacgtcc gggcgggtcg ccgagctggc gagcgcgatt cgcaggccgc gccggcgtag
      121 cgcctcgacc agctcgacgg ccccggggaa cgggcgcagc tgcgggacga agcgggccat
      181 cagggcctgg cggcgggtcg agaccgggtg gccctcgttg ctgtcgaggg cgacgccggc
      241 gagcgcgggc agcagcttgt cgctgcccat gccgacgagc ggccggacct gctcctgggt
      301 gacatcccag ccgaactcgg cgagggcctc gacccacgcg aggatgtggg cgtcgttgct
      361 gtcgacgagg gtcccgtcga ggtcgaagat cacgccgcgg atcgttggca caccggaccg
      421 atagcaccac ctcgcggacg cgccagtcga acgcgacgag tggcaggatc gcggcggtgc
      481 attaggctgg cggcgtgcgg atcggcgtcg acctcggggg cacgaagatc gagggcgccg
      541 tgctcgacgc ggccggcaag gtcgcgctgc gccggcggat cccgaccccc gccggcgact
      601 atggacagat cgtcgcgcgg atcgccgagg tcgtgctgcg gctcgagcgc gagtcgggca
      661 cgcgctgcac ggtcggcgtc ggtacaccag gctcggagaa cccctccacc gggctgatgc
      721 gcaactgcaa cgcgacgatc gtcaacggca aaccactgcg gcgcgacctc gaggccgcga
      781 tcggccggcc cgtgcgcctc gagaacgacg ccaactgctt cgcgctgtcg gaggcggtcg
      841 acggcgcggg cgcgggcgcg gcggtcgtgt tcggcgtgat cgtcggcacc ggcgtcggcg
      901 gcggcgtggt cgtgcgcggg cgcctgatct ccgggcgcaa ccggatcgcc ggcgagtggg
      961 gccacagccc gctgccgtgg gccgggccga acgagctgcc cgggcccctg tgtcactgcg
     1021 ggcgccgcgg atgcatcgag acgtttttat ccggcccggc gctggcccgg gacggcggcg
     1081 cggcgccgga gctgctggcg agctcgaccg acccgctcgc gcgcgccgcc ctcgatcgtt
     1141 acatcgaccg cctggcccgc gcgctggtgc cggtcattca tctgctcgac cccgatgtca
     1201 tcgtgctcgg cggtgggctg tcggcgatcg accgcctgta ccgcgacgtg ccgccgctgt
     1261 ggccaatgca cctgttctcg gacgcgccgc cgacgacgct gctgcgcccg gcgaagcacg
     1321 gggatgccag cggcgtgcgc ggggccgcgt ggctgtggca cgagccatag cccccgtcag
     1381 ggccaccacg tctcggcgac gaccgagccg ctggccgaag tgtgtagatt agcccccgtc
     1441 accaggacgg ccaggtttta cgccgtttca ctcctcgggc acgccaggat cggggatcag
     1501 gccgtgcttt cgcagcagcg tgcgcaggtg cttgcggtcg agctcggagc tgcgggcggc
     1561 cgccgagatg ttgccgtcga cccggtcgag cagggcacgc aaatacctgt gctcgaaggc
     1621 gtcgacgatc gcctgcttgg cggcggcgaa cgggcgttcg gtgtcgacct ccagaccgga
     1681 cttttcggac atgtccccgg ggacatacca gcgcagctcg gagaagctgc gcgccccggg
     1741 cgacagggcg agcgcccggt cgaccgcgtt gcgcagctcg cggacgttgc cgggccacgg
     1801 ctgcgcggtc agccgcgcga gcgccgggcc ctcgacgccc tcgctggcca atccccgctg
     1861 ctgcaacaga ctccgcacga tcggcgcgat gtcctcgcgg cgggcccgca gcggcggcag
     1921 ggtcacgcgc acgacgctga ggcgatagta gaggtccggc cgcagggccc cgctggcgac
     1981 cgcgccggcg aggtcgcgct ggcccgcggc gaccacccgc acgtcgatcg cctgctcgct
     2041 gtcaccgccg accggacgca cggtcctcgt ctcgagcgca cgcaacagcc gcggctggag
     2101 gtcgagcggc agcgcgtcga gctcgtcgag gaacagcgtg ccgccgtcgg cgcgggcgaa
     2161 cgcgccccgg cgggcctgca tcgcccc
//
LOCUS       DASWNJ010002035         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_634923, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002035 DASWNJ010000000
VERSION     DASWNJ010002035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634923"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VGB85_33860"
     CDS             <1..790
                     /locus_tag="VGB85_33860"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0139139.1"
                     /translation="AGPLPAFVIATLLGLDPALGVRFKAWADDFLSVTPEPPTAEHAA
                     RVKATIAELSAYMHRVITDRRRDPIDDTVSELVQAEVDGQRLGDAEILELMITILLGG
                     LETTTHLIASSAVLLAGQPELLARLRADRSLIPAFVEEMVRFDGPSQSLPRVTTTEVT
                     LSGVTIPAGSLVLAMVGSAGRDERRFPDPERFDIGRGSPALNFGHGIHFCLGAALSRM
                     ETQIALEALLGRFAGLELQTRELEYNRTMTVRGPVAVPLRLTPA"
     gene            845..1558
                     /locus_tag="VGB85_33865"
     CDS             845..1558
                     /locus_tag="VGB85_33865"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEY0139140.1"
                     /translation="MTGAARVLQRGRSTPVASLRPAAGQLALFGHEAPAIDLSFATLE
                     RTVLDHGAWIDVARGWLSGHVQLFNVLLEETRWRADDRVMYDRQVDVPRLYASGPTHG
                     PAAAQLQAMRAALDARYGTSFERTTLALYRDGRDSVAFHGDYIARRMDEALVATVSVG
                     APRRFLLRPTGGGRSHAFSCGWGDLLVMGGTCQRTYQHAIPKVAHADPRIAIMFRPIW
                     HEKPDPTSDAQDTAPRVDR"
     gene            1746..2030
                     /locus_tag="VGB85_33870"
     CDS             1746..2030
                     /locus_tag="VGB85_33870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139141.1"
                     /translation="MTRISAVLTILLFSTAACSGDSKSTGGANKGTAGAKDNKEAGAA
                     VGQAAYDSVESCLKSCDGPNESATDKATCRLNCDAAFGGNKRNAQPDGPR"
     gene            complement(2037..>2351)
                     /locus_tag="VGB85_33875"
     CDS             complement(2037..>2351)
                     /locus_tag="VGB85_33875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HEY0139142.1"
                     /translation="LPGTTGESDSDTSDDGDGDGDGDGTAGTGGSEATGVTTPATGDD
                     GGTPTSDGTPTSGTSVGGSEGSGDTGEQTGDGGCGCRSTPQPAHGALALLVLLGLRRR
                     RR"
BASE COUNT          324 a          849 c          860 g          318 t
ORIGIN      
        1 cgcggggccc ttgccggcgt tcgtgatcgc gaccctgctc gggctcgacc cggcgctcgg
       61 ggtccggttc aaggcctggg ccgacgactt tttatcggtc acaccggagc cgccgaccgc
      121 ggagcacgcg gcgcgggtca aggcgacgat cgcggagctc tcggcgtaca tgcaccgggt
      181 gatcaccgac cgcaggcgcg acccgatcga cgacaccgtc agcgagctcg tgcaggccga
      241 ggtcgacggg cagcgcctcg gcgacgcgga gatcctcgag ctcatgatca cgatcctgct
      301 cggcgggctc gagacgacga cgcacctgat cgccagctcg gcggtgctgc tcgccggcca
      361 gccggagctg ctcgccaggc tgcgcgccga tcgctcgctg atccccgcgt tcgtcgagga
      421 gatggtgcgc ttcgacgggc cgagtcagtc gctgccgcgg gtgacgacga ccgaggtgac
      481 gctctcgggc gtgacgatcc cggccggctc gctggtgctg gcgatggtcg gctcggcggg
      541 tcgcgacgag cggcgcttcc ccgaccccga gcgcttcgac atcggccgcg gcagccccgc
      601 cctcaacttc ggccacggga tccacttctg cctcggcgcg gcgctcagcc gcatggagac
      661 gcagatcgcg ctggaggcgt tgctcgggcg cttcgccggg ctcgagctgc agacccgcga
      721 gctcgagtac aaccggacga tgacggtgcg gggccccgtc gccgtgccgc tgcggctgac
      781 gcccgcgtga gctgactcgt cgttgacggg ccctggcggg ccacccgtgg caacttgtga
      841 agtcatgacg ggggcggcca gggtcctgca gcgcgggcgc agcacgccgg tcgcctcgct
      901 gcggcccgcg gccgggcagc tcgcgctgtt cggccacgag gcgccggcga tcgacctctc
      961 gttcgccacg ctcgagcgca ccgtgctcga ccacggcgcg tggatcgacg tcgcccgcgg
     1021 ctggctctcg ggacatgtgc aattgttcaa tgtcctgctc gaggagacgc gctggcgggc
     1081 cgacgaccgg gtgatgtacg accgccaggt cgacgtgccg cgcctctatg catccgggcc
     1141 gacgcatggg cccgcggcgg cgcagctgca ggcgatgcgc gccgcgctcg acgcccgcta
     1201 cggaacgagc ttcgagcgga ccacgctcgc gctctaccgc gacggccgcg acagcgtcgc
     1261 gtttcacggc gattacatcg cgcggcggat ggacgaggcg ctggtcgcga cggtctcggt
     1321 cggcgcgccg cggcggttct tgctgcgccc gaccggcggc ggccgctcgc atgcgttctc
     1381 gtgcggctgg ggcgacctgc tggtgatggg cgggacctgc cagcggacct atcaacacgc
     1441 gatcccgaag gtcgcgcacg cggatccgcg gatcgcgatc atgttccggc cgatctggca
     1501 cgagaagccc gatccgacga gtgacgcgca ggacaccgcg ccacgcgtcg atcgctgagg
     1561 ggggcagggg gcccgcgcac aggtcgatgg ggccagagat cccaccgggc atgccgggtg
     1621 tacggaagcg ttcgctgcgc actcgcccgg gtcgaggcag ccggcacgca cgatatcgac
     1681 gaaacggcgc gcattgtcgc gacacgcggc cggatcgggc catacaatcg ctgcaacagg
     1741 tacccatgac ccgaatctcc gccgtcctca ccatcctcct gttttccacg gccgcatgca
     1801 gcggcgactc caaatcgacc ggcggcgcca acaagggcac ggccggcgca aaggacaaca
     1861 aggaggccgg ggccgctgtg ggccaggcgg cgtacgacag cgtcgagagc tgcctcaagt
     1921 cgtgcgacgg gcccaacgag agcgcgaccg acaaggcgac gtgccgcctc aactgcgacg
     1981 cggcgttcgg cggcaacaag cgcaacgccc agcccgacgg cccgcgctga gccgcgtcag
     2041 cgacgacggc gacgcaggcc gagcaggacc agcagcgcga gcgcgccgtg ggccggctgc
     2101 ggcgtcgagc ggcacccgca gccgccgtcg ccggtctgct cgcccgtgtc accgctgccc
     2161 tcactgccgc cgacggacgt gcccgaggtc ggcgtgccgt ccgaggtcgg cgtgccgccg
     2221 tcgtcgccgg tcgcgggggt cgtgacgccg gtcgcctcgc tgccgccggt gccggccgtg
     2281 ccgtcgccgt cgccgtcgcc atcgccgtca tcggaggtgt cgctgtcgga ctcgccggtg
     2341 gtgccgggca g
//
LOCUS       DASWNJ010002036         3504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_634947, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002036 DASWNJ010000000
VERSION     DASWNJ010002036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634947"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1370)
                     /locus_tag="VGB85_33880"
     CDS             complement(<1..1370)
                     /locus_tag="VGB85_33880"
                     /inference="COORDINATES: protein motif:HMM:NF039983.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6079 family protein"
                     /protein_id="HEY0139143.1"
                     /translation="MTTIRDAYILPSPEDIRAMGFVVKLREADAASDEVKQLVKDYVI
                     TPAVEQAMPQILDDMKQVFDRGEDYGRFIHGSFGSGKSHFLTMLSLLIEGAAPAQDKF
                     RPLLAAHRESVRGKGREAADHAEWLERANLLVVRVHMLSVRGRTTSLDRAIYDGLNQA
                     LKRRGKQPFEFLNVDAIFAEVRREAQDYGDIVWKRLETAGIVGGREDFEAIASGSPQA
                     RERFARSWLAHKGRNPGDAGIDPRWSDGLKRMGEHARAQGFGGVVLMLDEFLLWLAEK
                     SGQEFVQEINNLNVIVDHNTGQRPAPIFVFVARQRNLKEFFPDLVDESKIHEHLDHHS
                     KRFEVTTLNDVELRHIVKGRVLQPRDPGAIAAAVGGLAVRHQKALPSLLAGGDLEYLQ
                     DVYPFHPALIEMLVDVTSLMQRERSALRMLYELLVVHYPDLQLGEFLPVGSAFAAIFP
                     EAGVEA"
     gene            complement(1367..2050)
                     /locus_tag="VGB85_33885"
     CDS             complement(1367..2050)
                     /locus_tag="VGB85_33885"
                     /inference="COORDINATES: protein motif:HMM:NF025468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HEY0139144.1"
                     /translation="MMRLLILLEGQTEEGFVKDVLAPHLLRFSVVVSYIVVTTRRDRL
                     TGEKRRGGGRWKHWKKDLLLWRFEHPGNDVRFTTLFDLYGLPDDFPELEAHTRDLDTV
                     RRAQKLQAAMASVVDDWRFIPYIQRHEFEALVLACLDQLSEWLDPNDRPGVQSLRREI
                     GDANPEDINDSPETAPSKRLERHIPGYIKTQHGPATIELAGLAAIRSKCPRFDAWLTK
                     LESLAGPTP"
     gene            complement(2047..3180)
                     /locus_tag="VGB85_33890"
     CDS             complement(2047..3180)
                     /locus_tag="VGB85_33890"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0139145.1"
                     /translation="MTGTKAITRIQVEGFCSLKSVDLVPGRITVLIGPNGAGKSNLLD
                     LLRMIPLMRTQSLRRFVGEAGGASALLHYGPKTTRVMSIRLEFQQDTGTNAYAANLGY
                     ASGDSLIFLDESIEHKSRAAPAAGRVATLGAGHAESRLEEEAQNGDNTAKTVRWWLSK
                     INFYHFHDTSLTSPLRQNARQADDKYLRSDGSNLAAFLRRLANSEAAEDIASWKRIGM
                     LIQKIAPYIKRLEPDLVDPANPVTSTVHLRWVDDRDYTFGVHDLSDGTLRAIALITAL
                     SQPVSRLPAFVTIDEPELGLHPAAITLLAGLVRSIAPHCQVLLATQSPALLDEFSADE
                     VVVAERQNGETVFQHLDAEKLKNWLEEYSLSELFDKNVLGGRP"
     gene            complement(3177..>3504)
                     /locus_tag="VGB85_33895"
     CDS             complement(3177..>3504)
                     /locus_tag="VGB85_33895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139146.1"
                     /translation="LYGWAGWTPLQRLKALLAIDEELEDAGIPLADRTGVLDSAWRLL
                     PDVAREDAPAAARLKAELQALVGPTGPSPESLEDWRRRFPPPKPPKKKTERARKAPRA
                     EEENDT"
BASE COUNT          567 a         1024 c         1259 g          654 t
ORIGIN      
        1 ctcgcctcga ccccggcttc ggggaagatg gcggcgaagg cggagccgac cgggaggaac
       61 tcgccgagct gaaggtccgg gtaatggacg acgagcagct cgtagagcat acgcagggcc
      121 gaacgctcgc gctgcatcag cgaggtgacg tcgaccagca tctcgatgag ggccgggtgg
      181 aagggataga cgtcttgcag gtattcgagg tcgccgccgg cgagcaggga gggcagcgcc
      241 ttttgatggc gcacggcgag gccgccgacg gcggcggcga tggccccggg gtcgcggggc
      301 tggaggacgc ggcccttgac gatgtggcgc agctcgacgt cgtttaatgt ggtgacctcg
      361 aagcgcttcg agtggtggtc gaggtgctcg tggatcttgc tctcgtcgac caggtcgggg
      421 aagaactcct tgaggttgcg ctggcgggcc acgaacacga agatcggcgc ggggcgctgg
      481 ccggtgttgt ggtcgacgat gacgttgagg ttgttgatct cctgaacgaa ctcctggccc
      541 gacttctcgg cgagccacag gaggaactcg tcgagcatga ggaccacgcc gccgaagccc
      601 tgggcgcggg cgtgctcgcc catgcgcttg aggccgtcgc tccagcgggg gtcgatgccg
      661 gcgtcgccgg ggttgcggcc cttgtgcgcg agccagctgc gggcgaagcg ctcgcgggcc
      721 tgtggggagc cgctggcgat ggcctcgaag tcctcgcggc cgccgacgat gccggcggtc
      781 tccaggcgct tccatacgat gtcgccgtag tcctgggcct cgcgccggac ctcggcgaag
      841 atcgcgtcga cgttgaggaa ctcgaagggc tgcttgccgc ggcgcttgag ggcctggttg
      901 aggccgtcgt agatcgcgcg gtcgaggctg gtggtccggc ctcggaccga tagcatatgc
      961 acgcggacga ccagcaggtt ggcgcgctcg agccactcgg cgtggtcggc ggcctcgcgg
     1021 cccttaccgc gcacggactc gcggtgggcg gcgagcagcg gacgaaactt gtcctgggcc
     1081 ggggccgcgc cctcaatcag gagcgacagc atcgtcagga agtgcgactt gccgctgccg
     1141 aagctgccgt ggatgaagcg cccgtagtcc tcgccgcggt cgaagacctg cttcatgtcg
     1201 tcgagaattt gcggcatcgc ctgctcgacc gcgggcgtga tgacgtagtc cttgacgagc
     1261 tgcttgacct cgtccgaggc cgcgtcggcc tcgcgcagct tgaccacgaa gcccatggcc
     1321 cggatgtcct ccgggctcgg gaggatgtat gcgtcgcgga tcgtggtcat ggggtgggtc
     1381 ctgcgagaga ttcgagcttg gtgagccagg cgtcgaagcg ggggcacttg ctgcgaatgg
     1441 cggccaggcc ggcgagctcg atggtggccg ggccgtgctg ggtcttgatg taacctggaa
     1501 tgtgtcgctc gagccgtttg gacggcgctg tctccgggct gtcgttgatg tcctcggggt
     1561 tggcgtcgcc gatctcgcgc cggagtgact gcacaccggg tcgatcattc gggtccagcc
     1621 actcggagag ctggtcgagg caagcgagga ccagcgcctc gaattcatgc cgctgaatgt
     1681 aggggatgaa gcgccagtcg tcgacgacac tggccatcgc cgcctgaagc ttctgggcgc
     1741 gacgcaccgt atcaaggtcg cgggtatgtg cttcgagctc cgggaagtcg tccggcagcc
     1801 cgtagagatc gaagagcgtc gtaaacctga catcattgcc ggggtgctcg aatctccaca
     1861 gcagcaggtc ctttttccag tgcttccagc ggccgccgcc gcgtcttttt tcgccggtca
     1921 gcctgtctcg gcgggtcgtg acgacgatgt agctcacaac gaccgagaac cgcagcaggt
     1981 gtggcgcgag gacgtctttg acgaacccct cctcggtctg gccctcgagc aggatgagca
     2041 ggcgcatcat ggccggcctc cgaggacgtt cttgtcgaac aattcggaga ggctgtactc
     2101 ttccagccaa ttcttgagct tctcggcgtc gaggtgctga aagactgtct cgccgttctg
     2161 gcgttctgcg actacgacct cgtcggccga gaactcgtcc aggagcgcgg gagattgcgt
     2221 ggccaggagg acctgacagt ggggcgcaat ggatcgaacg agacccgcca gcagcgtgat
     2281 cgcggcgggg tgcagcccga gctcgggttc atcgatcgtc acgaatgcgg ggaggcgcga
     2341 gacgggctgc gagagcgcgg tgatcagggc gatcgcgcga agcgtaccgt cagagagatc
     2401 gtggactccg aaagtgtagt ccctatcgtc gacccagcgc aaatgaaccg tagaagtgac
     2461 aggattggct gggtcaacta gatctggctc aaggcgtttg atatagggag cgattttttg
     2521 aatcaacatc ccaatgcgtt tccaggatgc aatgtcctca gcagcctcac tgttcgccaa
     2581 ccgtcgcagg aaggccgcga ggttactgcc atcggaacga aggtatttgt cgtctgcttg
     2641 ccgggcgttc tgtcgtaggg gggatgtaag cgatgtgtcg tggaagtgat agaaattaat
     2701 cttcgacagc caccaccgaa ctgtctttgc tgtgttgtcg ccattctgtg cctcctcctc
     2761 gaggcgcgat tcggcgtggc cggcgccgag ggtggccacc cttcctgccg cgggtgctgc
     2821 tcgcgacttg tgctcgatgg actcgtcgag aaaaatgagg ctgtcaccgg atgcgtagcc
     2881 aaggttggcg gcataggcat tcgtcccggt gtcctgctgg aattcgagac ggatgctcat
     2941 gaccctggtg gtcttcggcc cgtaatggag cagcgcagat gcgccccctg cttcgcccac
     3001 gaagcggcga agtgactgag tgcgcatgag cggaatcatg cgcaggaggt ccaagaggtt
     3061 cgacttgccg gctccgttgg gaccgatgag cacggtgata cgccccggaa ccagatcgac
     3121 gctcttgaga gagcaaaaac cctcgacttg gatgcgagtg atagctttag ttccggtcat
     3181 gtgtcgttct cttcctcggc acgcggggcc ttgcgggcgc gctcggtctt cttcttcggc
     3241 ggtttgggcg gcgggaagcg cctccgccaa tcttcgaggc tctcggggct cgggccggtg
     3301 gggccgacga gggcctgcag ctcggccttg agtcgagcgg cggcgggtgc gtcctcgcgg
     3361 gcgacgtctg gcagcagccg ccaggcgctg tcgagcacgc ccgtgcggtc ggcgagcggg
     3421 atgccggcgt cctcgagctc ctcgtcgatg gcgagcaggg ccttcagacg ctggagcggg
     3481 gtccagccgg cccagccgta gagg
//
LOCUS       DASWNJ010002037         1665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_350526, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002037 DASWNJ010000000
VERSION     DASWNJ010002037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350526"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..1123)
                     /locus_tag="VGB85_33900"
     CDS             complement(119..1123)
                     /locus_tag="VGB85_33900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY0139147.1"
                     /translation="MAQKKNEPAAGPRARVEMQFEQPHLMGPLFGEFDRNLVAIEDKL
                     GVYIAARGTKVQIEGEAEDAARARDVLQGLYNRLDEGQDIDAETVGAIISMSGQPVLD
                     GIISPDVAEPPKVMIRTRKKTIVPRTRTQGTYMEALARDDLIFALGPAGTGKTYLAVA
                     QAVQQLIGGSVDRLILSRPAVEAGERLGFLPGDMKEKVDPYLRPLYDALYDMLPTEQV
                     ERRIASGEIEIAPIAFMRGRTLSDAFIILDEAQNTTPQQMKMFLTRFGMRSRMVVCGD
                     PLQIDLPNPGMSGLADAVGRLEGISRIATIRFGAKDVVRHPLVGRIVEAYEGPDSAPR
                     "
     gene            complement(1123..>1665)
                     /locus_tag="VGB85_33905"
     CDS             complement(1123..>1665)
                     /locus_tag="VGB85_33905"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0139148.1"
                     /translation="LPVQSGSDRVLKAMNRSHDAAGYLRLLERVRAVRPDIALSGDFI
                     VGFPGETEAEFAETLALVATVNYAQAYSFKYSPRPGTPAADMDEQVAEEVKDERLQRL
                     QALLGAQQQGFNQATVGKRTNVLLERPGKRPGQLIGKSPWLQSVHLETGGAIGDLVEV
                     EILSAGPVSLAGAAIMQAAA"
BASE COUNT          254 a          596 c          541 g          274 t
ORIGIN      
        1 cgagacactg tgccgagcga agcgaggcat gctgagcccg agaaggttct cgacttcgct
       61 cgaaccggct tctcgacttc gctcgaagcg aacggagttg gtgcgcgcag gaagaggttc
      121 agcgcggcgc actgtccggc ccttcatacg cttccacgat ccgcccgacg agcggatggc
      181 ggaccacgtc cttcgcgccg aagcggatgg tggcgatccg gctgatcccc tccagccgcc
      241 cgaccgcatc ggcgagcccc gacatgcccg gattgggaag atcgatctgc agcggatcgc
      301 cgcacaccac catgcggctc ctcatgccga agcgggtcag gaacatcttc atctgctgcg
      361 gcgtggtgtt ctgcgcctcg tccaggatga tgaaggcatc ggacagggtc cgcccgcgca
      421 tgaaggcgat gggcgcgatc tcgatctcgc cgctcgcgat gcgccgctcg acctgctcgg
      481 tcggcagcat gtcgtagagc gcgtcgtaga gcgggcgaag atagggatcg accttctcct
      541 tcatgtcgcc gggcaggaag cccagccgct cgcccgcctc caccgcgggg cgcgagagga
      601 tcagccggtc gaccgatccg ccgatcaact gctgcaccgc ctgcgccacg gcgagatagg
      661 tcttgcccgt tcccgccggc ccgagcgcga agatcaggtc gtcgcgggcc aaagcctcca
      721 tgtaggtgcc ttgcgtgcgg gtgcgcggca cgatggtctt cttgcgggtg cggatcatca
      781 ccttgggcgg ctcggcgacg tccggcgaga tgatcccgtc cagcaccggc tgaccggaca
      841 tggagatgat cgcccccacc gtctcggcgt cgatgtcctg cccctcgtcg agccggttgt
      901 agaggccctg cagcacgtcg cgggcgcgcg ccgcatcctc cgcctcgccc tcgatctgca
      961 ccttggtgcc gcgcgcggcg atgtagacgc cgagcttgtc ctcgatcgcc accagattgc
     1021 ggtcgaactc gccgaacaga ggccccatca gatgcggctg ctcgaactgc atctcgacgc
     1081 gggcgcgggg cccggcggca ggctcgttct tcttctgggc catcaggcgg ccgcctgcat
     1141 gatcgcggcg ccggccaggc tgacggggcc cgcggacagg atctcgacct ccaccaggtc
     1201 gccgatcgcg ccgcccgtct ccagatgcac cgactgcagc cacggcgact tgccgatgag
     1261 ctggcccggc cttttgccgg gccgctccag cagcacattc gtccgcttgc ccaccgtcgc
     1321 ctggttgaag ccctgctgct gcgcgccgag cagcgcctgg agccgctgca gccgttcgtc
     1381 cttcacctct tccgccacct gttcatccat gtcggcggcg ggagtgcccg gccgcgggga
     1441 atatttgaac gagtaagcct gcgcatagtt caccgtcgcc accagagcga gcgtctccgc
     1501 gaactcggcc tccgtctcgc ccgggaaccc gacgatgaag tcccccgaca gggcgatgtc
     1561 gggacgcacg gcgcggaccc gctccagcag ccggagataa cccgccgcat cgtggctgcg
     1621 gttcatcgcc ttgagcaccc ggtcgctgcc cgactgcacg ggaag
//
LOCUS       DASWNJ010002038         2208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_422142, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002038 DASWNJ010000000
VERSION     DASWNJ010002038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422142"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="VGB85_33910"
     CDS             complement(<1..1304)
                     /locus_tag="VGB85_33910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139149.1"
                     /translation="MLPPSAARLSLVFLSLSACTGTDTGVSASTASSDTGAAGTSSTG
                     TGDPTPTTDAAPTTGDVPTTGEPGPGLASGPLQAGAAVGHITGPVGASMAGFGGRTKV
                     NDTAWNDVLNGASGFYGYGAAKAIALEVEGERLVLLKMPTMSSEASLTEGTLAKLKDL
                     HGIDLTGRLITGATHSHHNIARYWRLPPALGAVGADSPDEEIIDRVSTQLASVVKAAI
                     DDLGPAQWGTGFADDWDADDLVYRDRRNENDPTYGKDPRLTILAVRRPDGAPMAAVLN
                     FGMHGTVFDSDNELFTEDAPGGLEMKFEEHFFASTGKPIFGMFIQSGGGDASPAGDRL
                     GHPGPARVELLGHDAAPKILAVYDAIEWKDSVTLGVRSRRIDLTYDAIGYDEVPEFEN
                     AADYPYTWGGWRCNEGAGDEANPATSLEGQPKQCTDLATLLML"
     gene            1539..>2208
                     /locus_tag="VGB85_33915"
     CDS             1539..>2208
                     /locus_tag="VGB85_33915"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0139150.1"
                     /translation="MQCPTDGMMLPLEGRLLDHKFRLDRRVGDGGMASVWQATNTFVH
                     RTVAIKLMHERYAGDVELLGRFRNEASAAGKIGSQHICDVLDFGQSEIGPYIVMELLE
                     GCSLAELITTHRRLDPGLAVWIVRQALAGLEAAHRVGIVHRDLKPENIFLCRVIRGQW
                     LVKLMDFGISKFTQHAQTAAGTTMGTPHYMAPEQIQGAAKVDARADLWSIGVILYEAI
                     TSVQL"
BASE COUNT          303 a          731 c          819 g          355 t
ORIGIN      
        1 agcatcagca gcgtggcgag gtcggtgcac tgcttgggct gaccctcgag gctggtggcg
       61 gggttggcct cgtcgccggc gccctcgttg cagcgccagc cgccccaggt ataggggtag
      121 tcggcggcgt tctcgaactc ggggacctcg tcgtagccga tcgcgtcgta ggtgaggtcg
      181 atgcggcgcg agcggacgcc gagggtcacg ctgtccttcc attcgatcgc gtcatagacg
      241 gcgaggatct tcggcgcggc gtcgtggccg agcagctcga cgcgcgcggg cccggggtgc
      301 ccgaggcggt cgcccgcggg cgaggcatca ccgccgccgg actggatgaa catgccgaag
      361 atcggcttgc cggtgctcgc aaagaagtgc tcctcgaact tcatctcgag gccgcccggc
      421 gcgtcctcgg tgaacagctc gttgtcgctg tcgaacacgg tgccgtgcat gccgaagttg
      481 aggaccgcgg ccatcggcgc gccgtccggg cggcgcacgg cgaggatcgt caggcgcggg
      541 tccttgccgt acgtcgggtc gttctcgttg cgacggtcgc ggtacacgag gtcgtcggcg
      601 tcccagtcgt cggcgaagcc ggtgccccac tgcgcgggcc ccaggtcgtc gatcgcggcc
      661 ttgacgaccg aggccagctg cgtgctgacg cgatcgatga tctcctcgtc cggcgagtcg
      721 gcgccgacgg ccccgagcgc gggcggcagg cgccagtagc gggcgatgtt gtggtgcgag
      781 tgcgtcgcgc cggtgatcag ccggccagtg aggtcgatgc cgtgcaggtc cttgagcttt
      841 gcgagcgtgc cctcggtcag cgaggcctcg gagctcatcg tcggcatttt taatagaacg
      901 agccgctcac cctcgacctc gagcgcgatc gccttggcgg cgccgtagcc gtagaagccg
      961 gacgcgccgt tcaagacgtc gttccacgcg gtgtcgttga ccttggtgcg cccgccgaag
     1021 ccagccatcg aggcgccgac tggaccggtg atgtggccga ccgcggcgcc ggcctgcagg
     1081 gggccgctgg cgaggccggg cccgggctcg ccggtggtcg gtacgtcgcc ggtggtcggc
     1141 gcggcgtcgg tcgtcggggt cggatctccg gtgcccgtgg aggatgtgcc ggcggcgccg
     1201 gtgtcgctgc tggcggtgct cgcggacacg cccgtgtccg tgcccgtaca ggcggacagc
     1261 gacaggaata cgagggacag ccgggcggca gacgggggca gcatcgtccg cgacgatagg
     1321 ccgcggccgc gcgaaaaatc aatcttccat cgccccgcgc cggacacctg gatcctcgtg
     1381 accgcagggg ctgccgccta tcgtcgaccc tgcacggcgc ggagcattta gtacactcgc
     1441 cggcggtgcc gaccctgccg cctcctgccc ccgcgcccgc cggaggcccc gcgctcctgc
     1501 cgtgtcgtgg gtgcggtcgc gtgtactcgg cggagcggtt gcagtgtccg accgacggca
     1561 tgatgttgcc gctcgagggc cggctgctcg accacaagtt tcgcctcgac cggcgggtcg
     1621 gcgacggcgg gatggcgagc gtgtggcagg cgacgaacac cttcgtgcac cgcacggtcg
     1681 cgatcaagct gatgcacgag cgctacgccg gcgacgtcga gttgctgggc cggttccgca
     1741 acgaggcgtc ggcggcgggc aagatcggga gccagcacat ctgcgacgtc ctcgattttg
     1801 gtcagagcga gatcggaccc tacatcgtga tggagctgct cgagggctgc tcgctcgcgg
     1861 agctgatcac gacgcaccgc cgcctcgacc ccgggctcgc ggtgtggatc gttcgtcagg
     1921 cgctcgccgg tctcgaggcg gcgcaccgcg tcggcatcgt ccaccgcgac ctcaagccgg
     1981 agaacatctt cctgtgccgg gtgatccgcg ggcagtggct ggtcaagctg atggacttcg
     2041 ggatctcgaa gttcacccaa cacgcccaga cggccgccgg cacgacgatg ggcacgccgc
     2101 actacatggc gccggagcag atccaggggg ccgcgaaggt cgacgcgcgc gccgacctgt
     2161 ggtcgatcgg cgtgattctt tatgaggcga tcacgagcgt gcagctgt
//
LOCUS       DASWNJ010002039         3392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_422209, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002039 DASWNJ010000000
VERSION     DASWNJ010002039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422209"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1553
                     /locus_tag="VGB85_33920"
     CDS             90..1553
                     /locus_tag="VGB85_33920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HEY0139151.1"
                     /translation="MPTLSFAPNASSLVQGCRALLVVAPASTFASDAFPALLPGPAQA
                     LLLELAREQKPGDLGAVAATLTGTTEPRKLFVGVLPDGGSRYNSPARAESVRRVVAGA
                     DLGSSGKAGIVLVLADAAHLLPAVNAVGRAFPVYTSKSAPASTLAIHILAVDPSGQPL
                     PIGPEVEATLHATRTAAGLVDTPPSDLNPEAFAARAREELAGLANVEIKEIVGDALLA
                     ERLHGIHAVGRCATSAPRLLHASYTPPGGGGRHVALVGKGVTYDTGGLSIKISGAMVG
                     MKGDMGGAAAVLGAFKVLASSGCRHRLSLVLCIAENAVGPAAFKPDDILRMHSGKTVE
                     VNNTDAEGRLLLADGCSYAARVLGAEVILDAATLTGAQLMSTGLLHAAIISNDEALEH
                     LVIAAGKASGDLVHPLPFAPEFYKQEFESSVADMCNSVKNRMNAQSACAAQFVYWHID
                     DAQVRWCHVDLAGPSFPKNRGTGFGVALLSEVVRRLD"
     gene            1564..2256
                     /gene="ung"
                     /locus_tag="VGB85_33925"
     CDS             1564..2256
                     /gene="ung"
                     /locus_tag="VGB85_33925"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF003589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY0139152.1"
                     /translation="MYDGTSSSWTLPGSWASVLAEETGKQYFNDLVEFVNQERETSTI
                     FPPEEDVFRALDLTPFEQVRVVILGQDPYHDEGQGHGLCFSVLPGVKPPPSLANIFKE
                     LQADQGHDPPGHGHLVRWAEQGVLLLNTVLTVRAHSPGSHKNKGWEKFTDAVIRALSE
                     RYEPVVFALWGDFARKKASLIDEDRHRVVVAPHPSPIAAKGPRFVGSRPFTAINDALA
                     ELGSSGIDWNLA"
     gene            2339..2722
                     /locus_tag="VGB85_33930"
     CDS             2339..2722
                     /locus_tag="VGB85_33930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139153.1"
                     /translation="MDERRQARSENPWLAGDLYLQTVAQDHGLEHLTLATREGLLLSG
                     TPDRELNNRLAAVAPIYAEEPASLKPGMLEELAQGQPLQLWRVKIRERPFYLVGVGTP
                     TDMPEEVQTAFDRIFARPRSSRTSN"
     gene            complement(2813..>3392)
                     /locus_tag="VGB85_33935"
     CDS             complement(2813..>3392)
                     /locus_tag="VGB85_33935"
                     /inference="COORDINATES: protein motif:HMM:NF037140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HEY0139154.1"
                     /translation="TLVGATTRTALLSGPLRDRFQILEGIEFYGDDELAAIVLRTSGL
                     LGFAISPEAALEIGTRSRGTPRIANRLTRRVRDFAQVADEARGEIRRIELTDAARALK
                     ELGIDEAGLDALDRRMLTMIIENFRGGPVGVDALAASLGEPRDTLEGVVEPFLLQRGF
                     LLRTHRGRCATAKAARHLNLVLPAPAQGELEL"
BASE COUNT          504 a         1313 c         1095 g          480 t
ORIGIN      
        1 ctcgggcccg tcgcgcaagg ccgtgacgcg caacgccgcc ttgcggcgca gagcccccag
       61 gcgggccacg atcgggcata ctgccgcgcg tgccgaccct cagcttcgcc ccgaacgcat
      121 cctctctcgt gcagggctgc cgcgcgctcc tcgtggtcgc cccggccagc accttcgcca
      181 gtgacgcctt ccccgccctg ctccccggcc cagcccaggc gctgctgctc gagcttgccc
      241 gcgagcaaaa gcccggcgac ctcggggccg tcgcggcgac gctgaccggc acgaccgagc
      301 cgcgcaagct gttcgtcggc gtgctgccgg acggcggctc gcgctacaac tccccggcgc
      361 gggccgagag cgtgcgccgg gtcgtcgcgg gagccgatct cgggagctcg ggcaaggccg
      421 ggatcgtcct cgtcctcgct gacgccgccc acctgctccc ggccgtcaac gcggtcggtc
      481 gcgccttccc ggtctacacc tccaagtcag cgccggcctc gacgctcgcg atccacatcc
      541 tcgccgtcga cccctccggc cagccgctgc cgatcggccc ggaggtcgag gccaccctgc
      601 acgcgacccg cacggccgcc ggcctcgtcg acaccccgcc cagcgatctc aacccggagg
      661 ccttcgccgc ccgcgcccgc gaggagctcg ccggcctcgc caacgtcgag atcaaggaga
      721 tcgtcggcga cgcgctgctg gcggagcgcc tgcacgggat ccacgccgtc ggccgctgcg
      781 ccacctcggc gccccgcctg ctccacgcct cctacacccc gcccggcggc ggcggtcgcc
      841 acgtcgccct cgtcggcaag ggcgtcacct acgacaccgg cggcctgagc atcaagatca
      901 gcggcgcgat ggtgggaatg aagggcgaca tgggcggcgc cgcggccgtg ctcggcgcct
      961 tcaaggtcct ggccagcagc ggctgccggc atcggctgtc gctggtcctg tgcatcgcgg
     1021 agaacgcggt cggcccggcc gccttcaagc cggacgacat cctgcgcatg cactccggca
     1081 agaccgtcga ggtcaacaac accgatgcag agggtcgcct gctcctggcc gacggatgca
     1141 gctacgccgc ccgcgtgctc ggggccgagg tcatcctcga cgccgccacc ctcaccggcg
     1201 cccagctgat gtcgaccggc ctcctccacg ccgcgatcat cagcaacgac gaggccctcg
     1261 agcacctggt gatcgcggcc ggcaaggcga gcggcgatct cgtgcacccc ctgcccttcg
     1321 cgcccgagtt ttacaagcag gaattcgaga gcagtgtcgc ggacatgtgc aattcggtga
     1381 agaatcgcat gaacgcccag tccgcctgcg cggcgcagtt cgtgtactgg cacatcgacg
     1441 acgcccaggt ccgctggtgc cacgtcgacc tcgccggccc ctcgttcccg aagaaccgcg
     1501 gcaccggctt cggcgtcgcc ctgctgtccg aggtcgtgcg ccgcctcgac tagaaaggca
     1561 cccatgtacg acggcaccag cagcagttgg accctgcccg ggtcgtgggc ctccgtgctc
     1621 gccgaggaga ccggcaagca gtacttcaac gacctcgtcg agttcgtgaa tcaagaacgc
     1681 gaaacttcga ccattttccc gcccgaggag gacgtgtttc gggccctcga tctcaccccc
     1741 ttcgagcagg tccgcgtcgt gatcctcggc caggacccct atcacgacga gggtcagggc
     1801 catggcctgt gcttctcggt gctgcccggg gtcaagcctc ccccctcgct cgccaacatc
     1861 ttcaaggagc tccaggccga ccagggccac gacccacccg gccacggcca cctggtccgc
     1921 tgggccgagc agggcgtgct gctcctcaac accgtgctga ccgtccgggc ccacagcccc
     1981 ggctcgcaca agaacaaggg ctgggagaag ttcaccgacg ccgtgatccg cgcgctcagc
     2041 gagcgctacg agccggtcgt gttcgccctg tggggcgact tcgccaggaa gaaggcgagc
     2101 ctgatcgacg aggaccgcca ccgcgtcgtc gtcgccccgc acccctcgcc gatcgcggcc
     2161 aagggcccgc ggttcgtcgg cagtcggccg ttcacggcca tcaacgacgc gcttgccgag
     2221 ctcggcagct ccggcatcga ctggaacctc gcctgaccgt ctccctggat ccgctggttt
     2281 ttagcgtggg gcgaggaagg gatttatcct acacctcgca cacgggagaa aacggaccat
     2341 ggacgaacga cgtcaagcac gcagcgaaaa cccctggctc gccggggatc tgtacttgca
     2401 gacagtggcc caggaccacg gcctcgagca cctgaccctg gcgacgcgcg agggcctgct
     2461 gctctcgggc acgccggacc gggagctcaa caaccgcctc gccgcggtcg ccccgatcta
     2521 cgccgaggag cccgcgtcgc tcaagcccgg gatgctcgag gagctcgccc agggtcagcc
     2581 cctccagctg tggcgcgtga agatccgcga gcggccgttc tacctcgtcg gcgtcggcac
     2641 cccgaccgac atgcccgagg aggtccagac cgccttcgac cgcatcttcg cccgtccgcg
     2701 atcctcgcgt accagcaact gatccgctcc gagctccgcg ctccccgagc cccgccggcg
     2761 tctgcctgcg gggcttcgtc gttcgcggat ctgctcgcca ccgccaggca tctcagagct
     2821 cgagctcacc ctgcgccggg gcgggcagca ccaggttgag gtgccgggcc gccttcgctg
     2881 tcgcgcagcg gccgcggtgg gtccgcagca aaaatccccg ctgcaacagg aacggctcga
     2941 ccaccccctc gagggtgtca cgcggctcgc cgaggctggc cgcgagcgcg tcgaccccga
     3001 ccggcccgcc acgaaagttc tcgatgatca tcgtcagcat gcggcggtcg agcgcgtcga
     3061 ggccggcctc gtcgatgccg agctccttga gcgcccgcgc cgcgtcggtc agctcgatgc
     3121 gacggatctc gccgcgcgcc tcgtcggcga cctgcgcgaa gtcgcggacg cgtctggtca
     3181 gacggttggc gatccgcggc gtgccccggc tgcgcgtccc gatctcgagc gccgcctccg
     3241 ggctgatcgc aaagccgagc aggcccgagg tgcgcagcac gatcgccgcc agctcgtcgt
     3301 cgccgtagaa ctcgatgccc tcgaggatct ggaagcgatc gcgcagcggg ccgctgagca
     3361 gcgcggtgcg ggtggtcgcg ccaacgagcg ta
//
LOCUS       DASWNJ010002040         1574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_635129, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002040 DASWNJ010000000
VERSION     DASWNJ010002040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635129"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VGB85_33940"
     CDS             <1..824
                     /locus_tag="VGB85_33940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139155.1"
                     /translation="VEISRVTAATPALETQLRDAVATALGSSADFVTGGGLGGSLGLA
                     AWISASDQAIAREIPKAGGVVTPAVLFLHIELEVPATLRNQFDVPAITARTALSDTEP
                     TPEMLATAARSTLEVLELRLALARGEPEAAALLLRAEDPELVLLALEWTRDHPSPLLA
                     DAVAGQVEHSDPQVVRLALEILAEIGEARHASVVVRRVERWPALAREGYRTLAVLGGP
                     DAIGFLRFAAANEDESELRGEAERALDAALADSGERVASRRHGVDLPRVARGHRQ"
     gene            821..>1574
                     /locus_tag="VGB85_33945"
     CDS             821..>1574
                     /locus_tag="VGB85_33945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139156.1"
                     /translation="MSHRSPTSIFAVAGLLALVAGPALAQAPAVPPPTAPITPATPGS
                     EVVEAVPVSPPAETVPAAAPATAPAPAAAPAPATPAPTPYAGPGTTPPLTAPPGSAAP
                     RPYVAPGSDPAARPGFDFGGQALAPLPPPPAALDPGKIRRQPWRGRWWLALRMSVTGP
                     VAGEHPAAPSVLALGGGADFGWRVGNVLGLGMGLSGQTHNLKLLESLDLNTGLEGKRT
                     YKGQMLYWDALFARVHLPLKKRFQPYVEAGIGL"
BASE COUNT          181 a          582 c          605 g          206 t
ORIGIN      
        1 cggtggagat cagccgcgtg acggccgcca ccccggccct cgagacgcag ctgcgcgacg
       61 cggtggcgac ggcgctgggg agcagcgccg acttcgtcac gggcggcggg ctcggcgggt
      121 cgctcgggct cgcggcctgg atctcggcct cggaccaggc gatcgcgcgc gagatcccga
      181 aggccggcgg ggtggtcacg cccgcggtcc tcttccttca tatcgagctc gaggtgccgg
      241 cgacgctgcg caaccagttc gatgtcccgg cgatcacggc ccgcacggcg ctgtcggaca
      301 ccgagccgac cccggagatg ctggcgacgg cggcccgctc gacgctcgag gtgctcgagc
      361 tgcggctggc gctggcgcgc ggcgagccgg aggcggcggc gctgctgctg cgcgccgagg
      421 acccggagct ggtgctgctc gcgctcgagt ggacgcggga tcatccgagc ccgctgctgg
      481 ccgacgcggt cgcggggcag gtcgagcaca gcgacccgca ggtggtccgc ctggcgctcg
      541 agatcctggc cgagatcggt gaggcccggc acgcgtcggt ggtggtgcgt cgggtcgagc
      601 gctggccggc gctcgcgcgc gagggttacc ggaccctcgc ggtgctcggc gggcccgacg
      661 cgatcggctt cctgcggttt gccgcagcca acgaggacga gtcggagttg cgcggcgagg
      721 ccgagcgcgc cctcgatgcg gccctcgccg actccggcga acgtgtcgca tcgcgccggc
      781 atggtgtaga tcttccccgg gtggcgcgag ggcaccgaca atgagtcatc gttcaccgac
      841 atcgatcttc gcggtcgccg gtctgctcgc gctcgtcgcg ggcccggccc tggcccaggc
      901 gcccgcggta ccaccgccga ccgcaccgat tacgccggcg acgcccggca gtgaagtggt
      961 ggaggcggtc ccggtgtcgc caccggccga gacggtcccg gcggcggcgc ccgcgaccgc
     1021 gcctgctccg gcggcggcgc ctgctccggc gacgccggct ccgacgccgt acgcgggtcc
     1081 gggcacgacc ccgccgctga cggccccgcc gggatccgcg gcgccgcgtc cgtacgtggc
     1141 gccgggcagc gaccccgcgg cgcgccccgg cttcgacttc ggcggacagg cactcgcgcc
     1201 cttgccgcca ccgccagcgg cgctcgaccc gggcaagatc cgccgccagc cgtggcgcgg
     1261 tcgctggtgg ctggccctgc gcatgagcgt cacgggtccg gtcgcgggtg agcacccggc
     1321 ggccccctcg gtgctggcgc tcggcggcgg cgccgacttc ggctggcgcg tcggcaacgt
     1381 gctcggcctc ggcatggggc tctcggggca gacccacaac ctcaagctgc tcgagagcct
     1441 cgacctcaac accggcctcg agggcaagcg gacctacaag ggccagatgt tgtactggga
     1501 cgcgctgttc gcgcgggtgc acttgccgct caagaagcgg ttccagccgt acgtggaggc
     1561 cggcatcggc ctgg
//
LOCUS       DASWNJ010002041         4670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_635191, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002041 DASWNJ010000000
VERSION     DASWNJ010002041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635191"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VGB85_33950"
     CDS             <1..258
                     /locus_tag="VGB85_33950"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEY0139157.1"
                     /translation="TGLEALLREHGERRLWVLGLATDYCVKATALDALRLGFAVTLVV
                     DGCRAVDLQAGDGERAIAAMQAAGVTLATSAEVLADAAGAR"
     gene            272..1309
                     /locus_tag="VGB85_33955"
     CDS             272..1309
                     /locus_tag="VGB85_33955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139158.1"
                     /translation="MLLAFALSLAGLAEGLRLDWQAPADCPDAAQMRGKVVAMIGEPA
                     VSGADLQASGVVGVKDGRWTLELELQGAGGGDRRTLVDGECRALADAAALVIAVAIDP
                     QARAPVPEDSPAEAVVPAPPDVQEARGPEPAVPEGRGAPAPVPEDRVVAAPVPEDRPV
                     GEAPVERRRAVRLGLRVAGGVGFARILPAPHAMLELALGVEGRGWRAEIGGGFAPPVH
                     GTAATNPGIGGEFRLGLGEVRGCGMPGLARVPVEFPLCLGLQVGAMHGRGEGPGLAVT
                     YDVRALWVATRAGAAVRWRPRGGRFGLWLGLDAVVRLTRPAFVTAGEVQVHAAAPVGG
                     QASLGLEVLLR"
     gene            complement(1312..2778)
                     /locus_tag="VGB85_33960"
     CDS             complement(1312..2778)
                     /locus_tag="VGB85_33960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139159.1"
                     /translation="MSRPALLAPLLVLLAPACTPGGETTTTTTGFESGQATTEGPGPS
                     SFGDGSGTSTGDTPTTDPTTSTTDPTTDPTTDPSTGDPSTTTGTTSTTGTTTGELTTS
                     STDTGSSDTGAPVCGDGVVNGDELCDDGENDGDPCLADCTAGTAVLALVGAGTQPGIA
                     AMFTPGVGWASEPVSLGITEAELETTPGGAMAVVRRASAKPEEQGELFHARWQAGDPA
                     LFGMFATVGEFGFAKDGPSLAAVADTVTLAFLGTDNKHYTALYSNDAWATFTKLPAGM
                     VQLQAFGPSAAVVVPGIVETYAAYTGDDARIYYSSKSSPGGAWQASQGTPPPSVLGPP
                     VGVVDEEGDLLLAYVRKDDGRIALIKLLTPMNAWAKEATVHANAITASDIAFIRLATG
                     SYAIAWRGFDNDGIYLSLGTAYDSWGAVVTVEEPVVPTTRPALAHGVLGADLEILYGA
                     GGKLRHVRVADGKGIPAVDVPGITGATSVAATTVQLAP"
     gene            2907..3476
                     /locus_tag="VGB85_33965"
     CDS             2907..3476
                     /locus_tag="VGB85_33965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0139160.1"
                     /translation="MTADAPPRADVDAAGLATSLDLAEVYRCHAGFVWRVVRRLGVPD
                     AALEDVVHDVFLVVHRRLPEYDGRAALTTWLFGIARGVASNHRRGLARAQRKLAVVEP
                     PGGAADPEHATEQARVAAFVRDFLQGLDEDRRLLFELADIEGLKVPEIAETLGMNLNT
                     AYARLRAVREQLSRALAEREAGAGRSMAR"
     gene            3485..4258
                     /locus_tag="VGB85_33970"
     CDS             3485..4258
                     /locus_tag="VGB85_33970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139161.1"
                     /translation="MNTDDGLSPAARSALASLRADDDLPADARERVWARLAAAPVAAP
                     SDMSRRTGSRGAGLVIGLALAAGVALALVNLGRGAQQVAVAPQGDAAAYDRHGAAGIR
                     PVRVGEPPVRAAVAVIDLPAPVVVPLQVASPMSTSAREPARREAGLSDRAVRAETKPA
                     RALDPADASTLAAETALVQAAQAALAAGDPGTALARLDEHRRAHAGGVLARERDALRV
                     TALCDAGRRADSRAAAEQFMRQHPGSMLAERVQGVCVTP"
     gene            4363..>4670
                     /locus_tag="VGB85_33975"
     CDS             4363..>4670
                     /locus_tag="VGB85_33975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139162.1"
                     /translation="MTKLNNFFSISSLLLTAALGVTLGACDPKMIGAESEGDVECTDG
                     DTKPAGDGCNTCTCSDGDWACTELACEPDVCQPGETKQQDCNTCGCVEGGWACTVKDC
                     G"
BASE COUNT          546 a         1510 c         1971 g          643 t
ORIGIN      
        1 accggcctcg aggcgctgct gcgggagcac ggtgagcggc ggttgtgggt gctcgggctg
       61 gcgacggact actgcgtgaa ggcgacggcc ctcgacgcgc tgcgcctcgg cttcgcggtg
      121 acgctggtgg tcgacggctg ccgcgcggtc gacctgcagg cgggcgacgg ggagcgggcg
      181 atcgcggcga tgcaggctgc cggcgtcacg ctggcgacga gcgccgaagt gctcgccgac
      241 gcggccggcg ctcgctagac tgcgcgcccc gatgctgctc gccttcgccc tgtcgctcgc
      301 cggtctcgcg gagggcctgc gcctcgactg gcaggcgccc gcggactgcc cggacgcggc
      361 gcagatgcgc ggcaaggtgg tggcgatgat cggggagccg gccgtgagcg gcgcggactt
      421 gcaggcgagc ggcgtggtgg gggtaaagga cgggcggtgg acgctcgagc tcgagctgca
      481 gggggccggc ggcggcgacc ggcgtacgct cgtcgacggc gagtgccggg cgctggccga
      541 cgcggccgcg ctggtgatcg cggtggcgat cgacccgcag gcgcgggcgc ctgtcccgga
      601 ggacagcccg gccgaggccg tggtcccggc gccgccggat gtgcaggagg cgcgggggcc
      661 ggagccggct gtcccggagg gcagaggcgc gccggcacct gtccccgagg acagggtcgt
      721 ggcggcacct gtccccgagg acaggccggt tggcgaggcg ccggtcgagc ggaggcgagc
      781 ggtccggctc gggctgcggg tcgccggcgg ggtcgggttt gcgcggatct tgcccgcgcc
      841 gcacgcgatg ctggagctcg cgctcggggt cgaggggcgc gggtggcggg cggagattgg
      901 tggaggcttc gcgccgccgg tacacgggac ggcggccacg aacccgggga tcggcggcga
      961 gtttcgcctc ggccttggag aggtgcgcgg ctgcgggatg ccggggctgg cgcgcgtgcc
     1021 ggtcgagttt ccgctgtgcc tcggtttgca ggtcggcgcg atgcatggcc gcggtgaggg
     1081 cccggggctg gcggtcacgt acgacgtgcg ggcgctgtgg gtggcgacgc gggccggcgc
     1141 tgcggtgcgc tggcggccgc gtggcgggcg gttcgggctg tggctcgggc tcgacgcggt
     1201 cgtgcgattg acgcggccgg ccttcgtcac cgccggtgag gtgcaggtgc acgcggcggc
     1261 gccggtcggt ggtcaggcga gcctcggcct cgaggtcttg ctgcgctgaa gtcagggcgc
     1321 gagctggacc gtcgtcgcgg cgacggaggt cgcgccggtg atgccgggga cgtcgacggc
     1381 cgggatgccc ttgccgtcgg cgacgcggac gtggcggagc ttgccgccgg cgccgtagag
     1441 gatctcgagg tcggccccga gcacgccgtg ggcgagggcg gggcgagtgg tcggcacgac
     1501 cggctcctcg acggtgacga cggcgcccca gctgtcgtag gcggtgccga gggacaggta
     1561 gatgccgtcg ttgtcgaagc cgcgccaggc gatcgcgtag ctgccggtcg ccaggcggat
     1621 gaaggcgatg tcggaggccg tgatcgcgtt ggcgtgcacg gtcgcctcct tggcccaggc
     1681 gttcatcggg gtcaggagct tgatcagggc gatccggccg tcgtccttgc gcacgtaggc
     1741 gagcaggagg tcgccctcct cgtcgacgac gccgaccggg gggccgagca cgctcggcgg
     1801 cggggtgccc tgcgaggcct gccaggcgcc gccgggcgag gacttggagc tgtagtagat
     1861 ccgcgcgtcg tcgccggtgt acgcggcgta ggtctcgacg atgccgggca cgacgaccgc
     1921 ggcggaaggc ccaaaggcct ggagctggac catgccggcg ggcagcttgg tgaaggtcgc
     1981 ccacgcgtcg ttgctgtaca gcgcggtgta atgcttgttg tcggtgccga ggaaggcgag
     2041 cgtgacggtg tcggcgacgg cggcgagcga ggggccgtcc ttggcgaagc cgaactcgcc
     2101 gacggtggcg aacatgccga acagcgccgg gtcgccggcc tgccagcggg cgtggaacag
     2161 ctcgccctgc tcctcgggct tggcgctggc gcggcgtacg acggccatgg cgccgcccgg
     2221 cgtggtctcg agctcggcct cggtgatgcc gagcgagacg ggctcgctgg cccagccgac
     2281 gccgggcgtg aacatggcgg cgatgcccgg ctgcgtgccg gcgccgacga gcgcgagcac
     2341 ggcggtgccg gcggtgcaat ccgcgaggca ggggtcgccg tcgttctcgc cgtcgtcgca
     2401 gagctcgtcg ccgttgacga cgccgtcgcc gcaaaccggc gcgccggtgt cgctggatcc
     2461 ggtgtctgtg ctgctcgtgg tcagctcgcc cgtggtggta ccggtggtgg aggtggtgcc
     2521 ggtggtggtc gatggatcgc cggtcgatgg atcggtggtc ggatcggtgg tcgggtcggt
     2581 ggtcgacgtc gtcgggtcgg tggtcggggt gtcgccggtc gaggtgccgc tgccgtcgcc
     2641 gaagctgctc gggccggggc cttcggtggt cgcctggccc gactcgaagc cagtggtcgt
     2701 ggtcgtggtc tcaccgccgg gggtgcaggc gggggcgagg aggaccagca ggggggcgag
     2761 cagggcgggg cgcgacatgg tcgacctttt tcgtcgattt cgccgccggt ggccagcgcg
     2821 atcgcgcgcg ggcgatgaga gtatcgttat gcggtgggcg aggcacagaa gccgggacgg
     2881 ccggacaatc accgccggtg agcgccatga ctgccgatgc cccgccgcgc gcggacgtgg
     2941 acgccgccgg cctggcgacg agcctcgacc tggcggaggt ctatcgctgt cacgcgggct
     3001 tcgtgtggcg ggtggtgcgg cggctcgggg tgcccgacgc ggcgctggag gacgtggtcc
     3061 acgacgtgtt cctggtcgtg catcgccggc tgcccgagta cgacggccgg gcggcgctga
     3121 cgacctggct gttcggcatc gcccgcgggg tcgccagcaa ccaccgtcgc ggactcgcgc
     3181 gggcgcagcg caagctggcg gtggtcgagc cgccgggcgg ggcggcggac cccgagcacg
     3241 cgacggagca ggcgcgggtc gccgcgttcg tgcgtgattt tttgcagggg ctcgacgagg
     3301 accgccgcct gctgttcgag ctggcggaca tcgagggcct caaggtgccc gagatcgccg
     3361 agaccctggg gatgaacctc aacacggcgt acgcccgtct gcgtgccgtc cgcgaacaat
     3421 tgagccgcgc cctcgccgaa cgtgaggcgg gcgcgggcag gagcatggcc cgatgaatga
     3481 ccgcatgaac actgatgatg gcctgtctcc cgcggcgcgc tcggccctcg cgagcctgcg
     3541 ggcggacgat gacctgcccg ccgacgcgcg cgagcgcgtg tgggcccggc tcgccgcggc
     3601 gccggtggct gccccttcgg acatgtcgcg caggacaggc tcgcgcgggg ccgggctggt
     3661 gatcgggctg gcgctcgcgg ccggtgtggc gctcgcgctg gtcaacctcg gtcgtggggc
     3721 gcagcaggtc gccgtcgcgc cgcagggcga cgcggcggcg tacgacaggc acggggccgc
     3781 ggggatccgg ccggtgcggg tcggtgagcc gccggtgcgg gccgcggtgg cggtgatcga
     3841 cctgccggcg ccggtcgtcg tgccgttgca ggtcgcgtcg ccgatgtcga cgtccgcgcg
     3901 cgagccggcg cggcgcgagg ctggtctttc ggacagggcg gtacgcgcgg agacgaagcc
     3961 cgcgcgcgcg ctggatcctg cggacgcgtc gacgctggcg gccgagacgg cgctggtgca
     4021 ggcggcccag gccgcgctgg cggcgggcga cccggggacc gcgctggcgc ggctcgacga
     4081 gcaccgccgg gcgcacgcgg gcggggtgct ggcgcgcgag cgcgacgcgc tgcgtgtgac
     4141 ggcgctgtgc gacgcggggc gccgcgcgga cagccgggcg gcggcggagc agttcatgcg
     4201 ccagcacccg gggtcgatgc tggccgagcg ggtccagggc gtgtgtgtca cgccgtgacg
     4261 ccggcgtcgt gcgcggcgtc tcgatccgaa gatcgacgcg cggcacacag agacgcggcg
     4321 agccggacaa acaccgaaca gattccaacg acgagcgaca ccatgaccaa gcttaacaac
     4381 ttcttctcga tctcctccct cctgctcacc gccgctctcg gcgtcaccct cggcgcctgc
     4441 gacccgaaga tgatcggcgc cgagtccgag ggcgatgtcg agtgcacgga cggcgacacg
     4501 aagccggccg gcgacggctg caacacctgc acctgctccg acggcgattg ggcgtgcacc
     4561 gagctcgcct gcgagcccga cgtgtgccag cccggcgaga ccaagcagca ggactgcaac
     4621 acctgcggct gcgtggaggg cggctgggcg tgcaccgtca aggactgcgg
//
LOCUS       DASWNJ010002042         2352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_422430, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002042 DASWNJ010000000
VERSION     DASWNJ010002042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422430"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VGB85_33980"
     CDS             complement(<1..320)
                     /locus_tag="VGB85_33980"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HEY0139163.1"
                     /translation="MNKPLRVCAVSFLNAWPLVHPLTRQNLPGAPSFLDADGEPLFEV
                     STALPSECARQLEQGLCDVGLVPVAAYAAHPEWEVVPEVGIGCRGPVHSVVLVADCPL
                     EAIAR"
     gene            complement(336..1517)
                     /gene="mqnE"
                     /locus_tag="VGB85_33985"
     CDS             complement(336..1517)
                     /gene="mqnE"
                     /locus_tag="VGB85_33985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HEY0139164.1"
                     /translation="MTIRDIRRFATIADKVRNGVELDLDDGVFLYRYPDLLAVGALAN
                     EQRERLHGDHTFFNVNFHINPTNVCEADCKFCSFARLTPDSPAAYTMSVDQVRQKLVD
                     RLHQPVTEIHIVNGLHGGLPFEYYKDCLRVLKALRPDVHLKAYTAVEIHYFAEKFGMS
                     YEQVLRELIDAGLGSLPGGGAEIFAERVRHKICKDKATAEQWLEVHRTAHRLGLRSNC
                     TMLYGTIETLEERVDHMLRLRALQAETGGFQTFIPLAFHHEGNDFERLPEPTGVDNLR
                     TYAVGRLMLSNIAHIKAYWIMIGVKIAQVSLSFGVDDLDGTVQEEKIYHMAGAETPQA
                     MTRTDLVRLIREAGRTAVERDTLYNTRWTDDGKALDGIRLDASVPFSGHKDRVQLRVV
                     S"
     gene            complement(1531..2268)
                     /locus_tag="VGB85_33990"
     CDS             complement(1531..2268)
                     /locus_tag="VGB85_33990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139165.1"
                     /translation="MSSKPATADQFDDHGSALADVAALVALFSDPAVAALRASEARAA
                     GFGARVWVRPAPAHAMPALAGVIRGADFIDLSPATLADPPQPAVSDATGASRPVPPNM
                     SLATAPADPAWPAPATLSPGDRLVLRVEHDQLAAYCAWLVRAAAGPTNWTLTPFCPQP
                     GGLFRLHLIAAARLALPPQVRVEVRHDLHGVRLAQVALGFGADTLAGPIDAGRHLPLA
                     GVPRPSETSAAALCELVRQAGLEATTF"
BASE COUNT          352 a          722 c          943 g          335 t
ORIGIN      
        1 cgggcgatcg cctcgagcgg gcagtcggcg acgagcacga cgctgtgcac ggggccgcgg
       61 cagccgatgc cgacctccgg cacgacctcc cactcggggt gcgcggcgta ggcggcgacc
      121 ggcacgaggc cgacgtcgca caggccctgc tcgagctggc gcgcgcactc ggagggcagc
      181 gcggtgctga cctcgaagag gggctcgccg tcggcgtcga ggaagctcgg cgcgccgggc
      241 aagttctggc gcgtcagcgg gtggacgagc ggccaggcgt tgaggaacga gacggcgcag
      301 acgcgtaacg gcttgttcat aaaaggtctc cgatctcagg acacgacgcg cagctgcacg
      361 cggtccttgt ggcccgagaa cggcacgctg gcgtcgaggc ggatgccatc gagcgccttg
      421 ccgtcgtcgg tccatcgcgt gttgtagagg gtgtcgcgct cgacggcggt gcgcccggcc
      481 tcacggatca ggcgcacgag gtcggtgcgc gtcatcgcct gcggagtctc ggcgccggcc
      541 atgtgataga tcttctcctc ctggacggtg ccgtcgaggt cgtcgacgcc gaagctcagc
      601 gagacctggg cgatcttgac gccgatcatg atccagtagg ccttgatgtg ggcgatgttc
      661 gagagcatca ggcggccgac cgcgtaggtg cgcaggttgt cgacgccggt cggctcgggc
      721 aggcgctcga agtcgttgcc ctcgtggtgg aaggcgagcg ggataaaggt ctgaaagccg
      781 ccggtctcgg cctgcagcgc gcgcaggcgc agcatgtgat cgacgcgctc ctcgagcgtc
      841 tcgatcgtgc cgtagagcat cgtgcagttg ctgcgcaggc cgaggcggtg ggcggtgcgg
      901 tggacctcga gccactgctc ggcggtcgcc ttgtctttgc aaattttgtg gcgcacgcgc
      961 tcggcgaaga tctcggcgcc gccgccgggc aagctgccga ggccggcgtc gatcagctcg
     1021 cgcaggacct gctcgtagct catgccgaac ttctcggcga agtagtggat ctcgaccgcc
     1081 gtgtaggcct tgaggtgcac gtccgggcgc agggccttga gcacgcgcag gcagtccttg
     1141 tagtactcga agggcaggcc gccgtgcagg ccgttgacga tgtggatctc ggtgaccggc
     1201 tgatgcaggc ggtcgacgag cttctggcgc acctgatcga cgctcatcgt gtacgcggcc
     1261 ggcgagtcgg gcgtcagccg ggcgaacgag cagaacttgc aatctgcctc gcacacgttg
     1321 gtcgggttga tgtgaaagtt gacgttgaag aaggtgtggt cgccgtgcag gcgctcgcgc
     1381 tgctcgttgg ccagcgcgcc gactgcgagc agatcggggt agcggtacag gaacacgccg
     1441 tcgtcgaggt cgagctcgac gccgttgcgg accttgtcgg cgatcgtggc gaagcggcgg
     1501 atgtcgcgga tggtcatggg gagctctcct ttaaaaagtg gtcgcctcga ggccggcctg
     1561 gcggacgagc tcgcacaggg cggccgcgct ggtctcgctg gggcgcggga caccggcgag
     1621 cggcaagtga cggccggcgt cgatcgggcc ggcgagggtg tcggcgccga agcccagcgc
     1681 cacctgggcg aggcgcacgc cgtgcaggtc atgacggacc tcgacgcgca cctgcggcgg
     1741 caaggcgagg cgcgcggccg cgatcaggtg caggcgaaac aggccgcccg gctgcgggca
     1801 gaacggagtg agggtccagt tcgtcgggcc agccgcggcg cgcacgagcc aggcgcagta
     1861 ggcggcgagc tggtcgtgct cgacgcgcag aacgaggcgg tcgccgggcg acagggtggc
     1921 cggcgcgggc caggccgggt cggcgggagc tgtcgcaagg gacatgttcg gagggacagg
     1981 gcgcgaggcg ccggtcgcat cggacacagc cggctgcgga gggtccgcga gggtcgcggg
     2041 ggagagatcg atgaagtccg cgccgcggat cacaccggcg agcgcgggca tggcgtgcgc
     2101 gggggccggg cggacccaca cgcgcgcgcc gaagccggcc gcgcgggcct cggaggcgcg
     2161 cagggccgcg accgcgggat cggagaagag agcgacgagc gcggcgacgt cggccagcgc
     2221 ggagccgtga tcgtcgaact ggtcggcggt ggcgggcttg ctactcatgg tgtggctccc
     2281 cagcggcgca cgagcgggag ctcgaggccg gcgcggtcga gggcgcggac gacgacggtg
     2341 tcgatcgcgt cc
//
LOCUS       DASWNJ010002043         6328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_422451, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002043 DASWNJ010000000
VERSION     DASWNJ010002043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422451"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..2390
                     /locus_tag="VGB85_33995"
     CDS             210..2390
                     /locus_tag="VGB85_33995"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0139166.1"
                     /translation="MIVWLAGPVLEAMAAPPVLRPTPAARARPTARTTVRHDLRVKPE
                     NDNRAVSVVTRRDLEERLPRSAPDALRFEPGVYVQQTAHGQGSPYVRGLTGQQTIMMF
                     DGIRLNNSTFRQGPNQYFFTIDSRTVQKLEVLRGSASTRHGSDAMGGALLATPIDPAM
                     RLGSRKWQVHPRAMLRSGTADGELGGRAQVDVAYKDKVGFFGGVGYRDVGLLRTGGPL
                     HAPANGREVKVPLFAPDGVTQRGTGFKELTADARLVWQASDKLRLTLAYYDYRQFDAP
                     RTDRCPPATAPQNECLTYDEQYRTLVYTALDLRDGPAFAQTVRFTVNYQNQHERRRLD
                     HGPESVYRIIGRDDVHTAGTGLNVRTRTWGPAGKLRFSINYGLDVYHDTIVSKAWNVY
                     TDVGITSALSRGQYLDGGKYLTSGVWTEGKLEIFDRLELRAGGRGALVTAHAAGDPTS
                     ASKAVDRRFGAAVGGGGAALKLRDGLRWIVNADQGFRAPNMDDLTSRQQTGAGYQGEN
                     ADLRPEKSLSLETGIKLDLRRVEFALFGFQTMIDGLIARAPFDPANCPPGGETGCAGS
                     KAAFVLTNLDGRAMIRGFDGAVRLFLPAGFGIRATLAYAWGEGPNPVPGGSPTRLPLS
                     RVPPLNGTAEVSWRHEGFYAATALRWARLQDRLALSDLSDQRIPKGGTPGFAVVDLRA
                     GYRWQPHILLAGVLENLANAPYRYHGSSVNGPGRGFNLLLEFGF"
     gene            2410..3378
                     /locus_tag="VGB85_34000"
     CDS             2410..3378
                     /locus_tag="VGB85_34000"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY0139167.1"
                     /translation="MTTLPADIDPQDRESAASLAALWIGVALVLAAALTAAVLLIVGL
                     VGQRGDPFIEGEVPRDSPLYLRSTHRPAGVLHLAGSGSNLPLTRALADVFHEQNPGQH
                     VVVFDSIGSTGGVQAAYDDVIDLGLVSRPLKASEAALGMFVTPYARVPVVFAVNLGVP
                     DDSLTPAEVVEIYAGTRTRWSDGSPIVVLQRERGDSSHRAVSEQIPEFAAVNEAAYRE
                     ARWRVVDSDQAMQEALSITGGAVGLFDLGAIVLQRLPVRPLRISGIEPSEASVQSGRY
                     PFSKDLAFVSVEDPTSLASDFLRFVDGAQARKLMRASGYIPLPEDQ"
     gene            3375..4850
                     /locus_tag="VGB85_34005"
     CDS             3375..4850
                     /locus_tag="VGB85_34005"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY0139168.1"
                     /translation="MTGPEGHVPQDSERRRRQLGLRRQLGLRRQLGLRRYLAARMLPL
                     AVLLALLVSLSAPLAYFTLTARTLRTRAAATASQVASAVSAEIQERPVLWRYDSLKLL
                     AHIRAYTEERSIARIEVVDRSGVRVPLDDEPIGVVTGETLLWESAPIVINEQLVGHVW
                     AGAVLADARAGALLLLLPFGALGLGLAALVYFIPGRAMARAERRIDALITRLEQSQAS
                     LARFNQTLEHQVAARSSELKAAYEQLQAKEEHLRELSSRAVLLQEAERRVIARELHDS
                     AGQALTAIRINLQIIAQLAEVGPDALTPTSAATRAEKVARLAGRTLTIADATLEEIRR
                     AVSMLGPAILDDVGLVAAIQRLCDDFGERSDLRIDCELDDPPPAGLSPAIESVCYRVA
                     QEALTNVARHAEATRVHVRLTIGPQISLEVRDNGRGFTPGGAPRPTIGGRGLVGMRER
                     VELLGGTIRIETSPGSGTCVTVDLPQVTLSDDGDDTAIVVA"
     gene            4863..5534
                     /locus_tag="VGB85_34010"
     CDS             4863..5534
                     /locus_tag="VGB85_34010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0139169.1"
                     /translation="MSTARAPIRVVLAEDHTIVREGLRALLDVRDDVEVVAEVSDGRA
                     AVEAVAEHKPDVVVMDLGMPELNGVDATRQIRKEYPQTQVLILSMHSGEEYVRPAIRA
                     GASGYLLKGSGLSDLVAAIQAVARGDAFFSPAIAKIVLFESRRTDRPGKDPAPGDALT
                     NREREILQLVAEGKSSPEIAKQLQLSVKTVEGHRGRIMAKLDVHHVAGLVRHAIRIGL
                     VSADS"
     gene            5648..>6328
                     /locus_tag="VGB85_34015"
     CDS             5648..>6328
                     /locus_tag="VGB85_34015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139170.1"
                     /translation="MNTRVHRYTLSLLLIPLLGGCKAEDPEDTEDTANTETASGTTDE
                     PTGEPTTGTPLECSGLEVPAIDESSCKPLATDYQPRVNGSADDSWPACAGDTLPYQQV
                     EAKTPGSAARIDAYVEMAAALWDNAEPTAEDFTQARDQYLIPEGLASRVVRREDDHYP
                     EIPMAEWDPQVDGDKQCTVAALAAKYTDRCVGPNHMQPIIDAAFAAGQKGEGDLRVHA
                     AKIHATLDW"
BASE COUNT          932 a         2422 c         2103 g          871 t
ORIGIN      
        1 aatggggcta ctgcgaggag ccggaagccg gaactcccga gcatgagttg ctggtcgagc
       61 tgcgccgccc cgaggtcggg gacgagccgc acggctagca cgaccaggta gatccacctg
      121 caatgcccgg gaggtttgcc ccgggtccgg ccagccgcga caatgcggcc cccgtggtcc
      181 cccgtgtgtg tcgcctggcc tgcctcgcca tgatcgtgtg gctcgcggga cctgtcctcg
      241 aggccatggc cgcgccgccc gtgctgcggc cgaccccggc cgcgcgcgcc cgacccaccg
      301 cgcgcacgac cgtccgccac gacctccgcg tcaagccgga gaacgacaac cgggccgtca
      361 gcgtggtcac gcgccgcgac ctcgaagaac gcctgccgcg ctccgccccc gacgcgctgc
      421 gcttcgagcc cggcgtgtac gtgcaacaga cggcgcacgg ccagggctcg ccgtacgtcc
      481 gcggcctgac cggccagcag acgatcatga tgttcgacgg catccgcctc aacaacagca
      541 cctttcgtca gggccccaac cagtacttct tcacgatcga ctcgcgcacc gtgcaaaagc
      601 tcgaggtcct gcgcggcagc gcctcgaccc gccacggctc cgacgcgatg ggcggcgcct
      661 tgctggcgac gccgatcgac ccggcgatgc gcctcggctc gcgcaagtgg caggtccacc
      721 cgcgcgccat gctgcgctcc ggcaccgccg acggcgagct cggcgggcgt gcgcaggtcg
      781 acgtcgccta caaggacaag gtcggcttct tcggcggcgt cggctaccgc gacgtcggcc
      841 tgctgcgcac cggcggcccg ctccacgccc cggccaacgg gcgcgaggtc aaggtcccgc
      901 tgttcgcccc cgacggcgtc acgcagcgcg gcaccggctt caaggagctg accgccgacg
      961 cgcgcctcgt ctggcaggcg agcgacaagc tgcgcctgac gctcgcttac tacgactacc
     1021 gccagttcga cgccccccgc accgaccgct gcccgcccgc gaccgcgccg cagaacgagt
     1081 gcctgaccta cgacgagcag taccgcacgc tcgtgtacac cgcgctcgac ctgcgcgacg
     1141 gcccggcctt cgcgcagacg gttcgtttca ccgtcaacta tcagaaccag cacgagcgtc
     1201 gccgcctcga ccacgggccc gagtcggtct accgcatcat cggccgcgac gacgtgcaca
     1261 ccgcgggcac cggcctcaac gtccgcacgc gcacctgggg tccggccggc aagctgcgct
     1321 tttcgatcaa ctacggcctc gatgtctatc acgacacgat cgtcagcaag gcgtggaacg
     1381 tctacaccga cgtcggcatc accagcgcgc tcagccgcgg gcagtacctc gacggcggca
     1441 agtacctgac ctcgggcgtg tggaccgagg gcaagctcga gatcttcgac cgcctggagc
     1501 tgcgcgccgg cggccgtggg gcgctcgtca cggcccacgc cgcgggcgac ccgacgagcg
     1561 cctcgaaggc cgtcgaccgc cgcttcggcg ccgcggtcgg tggcggcggc gcggcgctca
     1621 agctgcgcga cgggctgcga tggatcgtca acgccgacca gggctttcgc gcgcccaaca
     1681 tggacgacct gaccagcagg cagcagacgg gcgccgggta tcagggcgag aacgccgacc
     1741 tgcggcccga gaagtcgctg tcgctcgaga ccgggatcaa gctcgacctg aggcgcgtcg
     1801 aattcgcgct gttcgggttc cagacgatga tcgacggcct gatcgcccgc gcccccttcg
     1861 accccgccaa ctgcccgccc ggcggcgaga ccggctgcgc cgggtcgaag gccgcgttcg
     1921 tgctgaccaa cctcgacggc cgcgcgatga tccgcggctt tgacggcgcc gtgcggctgt
     1981 tcctgcccgc cggcttcggc atccgcgcga ccctcgcgta cgcctggggc gagggcccca
     2041 accccgtccc cggcggatct cccacgcggc tgccgctgtc acgcgtcccc ccgctcaatg
     2101 gcaccgccga ggtctcgtgg cgccacgaag ggttctacgc ggccaccgcc ctgcgctggg
     2161 cccgattaca ggaccgcctc gccctctccg acctctccga ccagcgcatc cccaagggcg
     2221 gcacccccgg cttcgccgtc gtcgacctgc gcgccggcta ccgctggcaa ccccacatcc
     2281 tcctcgccgg cgtgctcgag aacctcgcca acgcgccgta tcgttaccac ggctccagcg
     2341 tcaacggccc cggccgcggc ttcaatctcc tcctcgaatt cggcttttga tcgcgatctc
     2401 ccggacacca tgaccacgct gccagccgac atcgacccgc aggaccgcga gtcggccgcg
     2461 tcgctcgccg ccctgtggat cggcgtcgcc ctcgtgctcg cggccgcgct gacggccgcg
     2521 gtgctgttga tcgtcggcct ggtcggccag cgcggcgacc cttttataga gggcgaggtc
     2581 ccgcgcgact cgccgctgta cctgcgctcg acgcatcgcc ccgcgggcgt gctgcacctc
     2641 gcgggctcgg gcagcaactt gccgctgacg cgcgcgctgg ccgacgtgtt ccacgagcag
     2701 aaccccgggc agcacgtcgt cgtgttcgac agcatcgggt cgaccggcgg tgtgcaggcg
     2761 gcctacgacg acgtgatcga cctcggcctg gtgtcgcgcc cgctcaaggc cagcgaggcc
     2821 gcgctcggca tgttcgtcac gccctacgcg cgcgtgccgg tcgtgttcgc cgtcaacctc
     2881 ggggtcccgg acgactcgct gacgcccgcc gaggtcgtcg agatctacgc cgggacccgg
     2941 acccggtgga gcgacggcag cccgatcgtc gtgttgcagc gcgagcgcgg cgactcgagc
     3001 caccgcgcgg tctccgagca gatccccgag ttcgccgccg tcaacgaggc cgcgtaccgc
     3061 gaggcccgtt ggcgcgtcgt cgacagcgac caggcgatgc aggaggcgct gagcatcacc
     3121 ggcggcgccg tcggcctgtt cgacctcggc gcgatcgtcc tccagcgcct gcccgtgcgg
     3181 ccgctgcgga tctccggcat cgagccctcc gaggccagcg tgcagtcggg gcgctacccc
     3241 ttctcaaaag acctcgcgtt cgtcagcgtc gaggacccca ccagcctcgc ctccgacttc
     3301 ctgcgcttcg tcgacggcgc ccaggcccgc aagctcatgc gcgccagcgg ctacatcccg
     3361 ctcccggagg accaatgacc gggcccgagg gacatgtccc gcaggacagc gaacgccgac
     3421 gccgccagct tggcctgcgg cgccagctcg ggctgcgccg ccagctcggc ctgcggcgct
     3481 acctcgccgc gcgcatgctg ccgctcgccg tgctgctcgc gctgctcgtc tcgctgtcgg
     3541 cgccgctggc ctacttcacg ctgacggcgc ggaccttgcg gacgcgggcc gccgcgaccg
     3601 cctcgcaggt cgcgtcggcc gtcagcgccg agatccagga gcggccggtg ttgtggcgct
     3661 acgactcgct caagctgctc gcgcacatcc gcgcgtacac cgaggagcgc agcatcgccc
     3721 gcatcgaggt cgtcgaccgc agcggcgtgc gcgtgccgct cgacgacgag ccgatcggcg
     3781 tcgtcaccgg cgagaccctg ctgtgggagt cggcgccgat cgtcatcaac gagcagctcg
     3841 tcgggcacgt gtgggccggg gccgtgctgg ccgacgcgcg cgccggggcc ctgctgttgc
     3901 tgctgccctt cggcgcgctc ggcctcggcc tcgccgcgct cgtctacttc atccctggtc
     3961 gggcgatggc ccgcgccgag cggcgcatcg atgcattgat cacccgcctc gagcagagcc
     4021 aggccagcct cgcgcgcttc aaccagactt tggagcacca ggtcgccgcg cgctcgtccg
     4081 agctcaaggc cgcgtacgag cagctccagg ccaaggagga gcacctgcgc gagctgtcct
     4141 cgcgggccgt gctgctgcag gaggccgagc gccgggtgat cgcccgcgag ctgcacgact
     4201 ccgctggcca ggcgttgacg gcgatccgca tcaacctcca gatcatcgcg cagctcgccg
     4261 aggtcggccc cgacgcgctc accccaacct ccgcggcgac gcgcgccgag aaggtcgccc
     4321 gcctcgccgg ccgcacgttg acgatcgccg acgccacgct cgaggagatc cggcgcgcgg
     4381 tgagcatgct cggcccggcg atcctcgacg acgtcggcct ggtcgccgcg atccagaggc
     4441 tgtgcgacga cttcggcgag cgctccgacc tgcgcatcga ctgcgagctc gatgacccgc
     4501 ccccggccgg cctgagcccc gcgatcgaga gcgtgtgtta tcgcgtcgcc caggaggccc
     4561 tgaccaacgt cgcccgccac gccgaggcca cgcgcgtgca tgtgcgcctg acgatcggcc
     4621 cgcagatctc gctcgaggtc cgcgacaacg gccgcggctt cacccccggt ggcgccccca
     4681 ggccgaccat cggcggtagg ggacttgtcg ggatgcgcga acgggtggaa ctactgggtg
     4741 gcacgatccg catcgaaacg agccccggaa gcggaacctg cgtcacggtc gacttgccgc
     4801 aggtgaccct ctccgacgac ggcgacgaca ccgcgatcgt ggtcgcgtga tatggtccga
     4861 ccgtgtccac cgctcgcgcc ccgatccgcg tcgttctggc cgaagaccac accatcgtgc
     4921 gtgagggcct gcgcgccctg ctcgacgtgc gcgacgacgt cgaggtggtc gccgaggtct
     4981 ccgacggccg ggccgcggtc gaggccgtcg cggagcacaa gcccgacgtc gtcgtgatgg
     5041 acctcggcat gcccgagctc aacggcgtcg acgcgacgcg ccagatccgc aaggagtacc
     5101 cgcagaccca ggtcctgatc ctgtcgatgc actccggcga ggagtacgtg cgcccggcga
     5161 tccgcgcggg cgccagcggg tatctactca aggggtcggg cctgtcggac ctcgtcgccg
     5221 cgatccaggc ggtcgcgcgc ggtgacgcct tcttcagccc cgcgatcgcc aagatcgtgc
     5281 tgttcgagtc gcggcgcacc gaccggcccg gcaaggaccc cgcccccggc gacgccctga
     5341 ccaaccgcga gcgcgagatc ctgcagctcg tcgccgaggg caagtcgagc ccggagatcg
     5401 ccaagcagct gcaattgagc gtaaagaccg tcgagggcca ccgcggacgc atcatggcga
     5461 agctggacgt gcaccacgtc gccgggctcg tacgtcatgc gatccgcatc ggcctggtct
     5521 ccgcggactc gtgagcgcgg ttttcgttcc ggagatccac ccgcagtttc cgcccgtgga
     5581 agccccgtag aggccgggcg aggccgcctc taaggtgcag cgaaatcctg tcgctggtga
     5641 ccttgaaatg aatacacgcg tccatcgtta caccctctcg ctcttactga tcccgttact
     5701 tggtggctgt aaggccgagg acccggaaga caccgaggac accgccaaca ccgagaccgc
     5761 cagcggcacg accgacgagc cgaccggcga gccgaccacc ggtacgccgc tcgagtgctc
     5821 gggcctcgag gtcccggcga tcgacgagag ctcgtgcaag ccgctcgcca ccgactatca
     5881 gccgcgcgtc aacggctcgg cggacgacag ctggccggcc tgcgccggtg acacgctgcc
     5941 gtaccagcag gtcgaggcga agacccccgg gtcggcggcc cgcatcgacg cctatgtgga
     6001 gatggcagcg gcgttgtggg acaacgccga gccgaccgcg gaggacttca cccaggcgcg
     6061 cgaccagtac ctgatccccg agggcctcgc gtcgcgcgtg gtccggcgcg aggacgacca
     6121 ctatccggag atcccgatgg ccgagtggga cccgcaggtc gacggtgaca agcagtgcac
     6181 ggtggccgcg ctcgccgcca agtacacgga ccgctgcgtc ggccccaatc acatgcagcc
     6241 gatcatcgac gcggcgttcg ccgccggcca aaagggcgag ggcgacctgc gcgtccacgc
     6301 cgcgaagatc cacgcgacgc tcgactgg
//
LOCUS       DASWNJ010002044         3352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_635382, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002044 DASWNJ010000000
VERSION     DASWNJ010002044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635382"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VGB85_34020"
     CDS             complement(<1..966)
                     /locus_tag="VGB85_34020"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY0139171.1"
                     /translation="MAGQNQLDLFGGAAAPQAPASNSKGRAVQGPVGPAEHPPELGTL
                     AAAMPAGLRMGTSSWAFTGWAGLVYDKAHPQNRLSREGLAAYAQHPLLRGVGVDRSYY
                     APLAAEEFAAYAAVVPAGFRFLVKAHEACTIAVYPRHARYGALRGQPNSRFLDAVYAR
                     EAVIGPTLAGLGDKAGPLLFQFSPTDVEALGGPRWFADRLQRFLSDLPRGPLYAVEIR
                     NRELLTGAYSDALASCGVCHCINVYPGMPMPRVQFERTRQDLAPAVVCRWMLHPRMNW
                     EQAETSYAPFDRIVDADEERRQDMARVCLRAEALGLPTQVIVNNNA"
     gene            complement(1158..2186)
                     /locus_tag="VGB85_34025"
     CDS             complement(1158..2186)
                     /locus_tag="VGB85_34025"
                     /inference="COORDINATES: protein motif:HMM:NF015915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY0139172.1"
                     /translation="MEALPLESLGRLDSRLDSRLDPGSEVLERAPDARATSAQAMSSM
                     SSMSTASGHPGQLNLAPLTPVPDLAPEPERPVDPPWGKVRASALASAVAGFDKLARYH
                     RLEVRGLDRIPVDGPALLVGNHNGGLNPVDGLFLVHYYRTLGYDRPVYILAHDILFRV
                     KKMAQVLESVGIIRARKGQARELLAAGHKVLVFPGGDIESMRPFRERRKIVLAERHGF
                     IHLALRTGAPIVPVVSAGSHETLLILSQGTRMARLMNLPRLARLNSLPLLLAAPWGLM
                     WGPTCALPYLPLPAKITVQIGEPVTVDRCEEPQVHERTPPIYEHVQSSMQAILDDLYS
                     ERLFPILG"
     gene            complement(2207..>3352)
                     /locus_tag="VGB85_34030"
     CDS             complement(2207..>3352)
                     /locus_tag="VGB85_34030"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0139173.1"
                     /translation="PTPRGAQSGHRSPNARVPRGQLGAKRRRNFCRRAAHRPPPAQAF
                     ARSIRDGQHYYVPPFLGLFDHAIVTTNADMLTVPERIARRVLNAQGLRSRFIETRVGR
                     MHVLDSTGGGNLPPIVMLHGLSANAICYAGLLRRFAPHTRRVIAPDMPAHGFSAVPVG
                     GLTDTAMVTALSEVLDRVLAESSEPAILVGNSMGGLAAIRYAIARPERVAALALVSPG
                     GAPMPAAQFAKFLATFDISSHRQALDFVDAVFANGAGVLRHALAHGIRQRFTHPQLRQ
                     LLASITPEQMLAPHELSGLRIPTLLVWGREERVLPRTHLEFFRKHLPSHAEIEEWPNF
                     GHIGFLEQPDALAARILGFARATAAGAKNLSRRSTGCGDYATLRVSA"
BASE COUNT          519 a         1054 c         1312 g          467 t
ORIGIN      
        1 ggcgttgttg ttgacgatga cctgcgtcgg gagtccgagc gcctcggcgc gcaggcacac
       61 gcgggccatg tcctggcggc gctcctcgtc ggcgtcgacg atgcgatcga agggggcgta
      121 ggaggtctcg gcctgctccc agttcatgcg cgggtggagc atccagcggc acacgacggc
      181 gggcgcgagg tcctgccgcg tgcgctcgaa ctggacccgc ggcatcggca tgcccgggta
      241 cacgttgatg cagtggcaca cgccgcagct ggcgagcgcg tcgctgtagg cgccggtcag
      301 cagctcgcgg ttgcggatct cgaccgcgta gagcgggccg cgggggaggt cggagaggaa
      361 gcgctgcagg cggtcggcga accaccgcgg gccgccgagc gcctcgacgt ccgtcgggga
      421 gaattgaaac agcagcggtc cggccttgtc gccgaggccg gcgagggtcg ggccgatgac
      481 ggcctcgcgc gcgtagacgg cgtcgaggaa gcgcgagttc ggctgaccac gaagcgcccc
      541 gtagcgggcg tgtcgcggat acacggcgat cgtgcaggcc tcgtgcgcct tgacgaggaa
      601 gcgaaagccc gcgggcacca ccgcggcgta ggccgcgaat tcttccgcgg cgagcggcgc
      661 gtagtagctg cggtcgacgc cgacgccgcg cagcagcggg tgctgggcgt aggcggcgag
      721 gccctcgcgc gacaggcggt tttgcgggtg ggccttgtcg tagacgagcc cggcccagcc
      781 ggtgaaggcc cacgacgagg tgcccatgcg caggccggcg ggcatcgccg ccgcgagcgt
      841 gccgagctcc ggcgggtgtt ccgcggggcc gacgggtcct tgcacggcgc ggcccttgct
      901 gttgctggca ggcgcctgcg gggccgcggc cccgccgaag aggtcgagct gattctggcc
      961 tgccatgcgg ttaaaggcag gctatcgcaa aatcgcgcgc gcgcaagcgc gggtcgcagc
     1021 gacgtcgttc gcagccccgg ccgccgcggg tgaggctgtc tcgtgggaca ggcgccccgg
     1081 agggtcccgg atcaaagcga ccgactgtcc cggaggtccg ctgcttccag gtcagaccgg
     1141 ctgtctcggg ggacaggtca gccgaggatc gggaacaggc gctcgctgta cagatcgtcg
     1201 aggatcgcct gcatcgagga ctggacgtgc tcgtagatcg gcggggtgcg ctcgtggacc
     1261 tgcggctcct cgcagcggtc gaccgtgacg ggctcgccga tctgcacggt gatcttggcg
     1321 ggcaggggca ggtagggcag cgcgcaggtc gggccccaca tcaggcccca cggggcggcg
     1381 agcagcagcg gcagcgagtt gaggcgggcc agccgcggca ggttcatcag gcgggccatg
     1441 cgggtgccct gcgagaggat cagcagggtc tcgtgcgagc cggcgctgac gaccggcacg
     1501 atcggtgccc cggtgcgcag cgcgaggtgg ataaagccgt ggcgctcggc gagcacgatc
     1561 ttgcggcgct cgcggaacgg tcgcatcgac tcgatgtcgc cgcccgggaa caccagcacc
     1621 ttgtgacccg ccgccagcag ctcgcgcgcc tgacccttgc gggcgcggat gatgccgacc
     1681 gactcgagca cctgcgccat cttcttgacg cgaaacagga tgtcgtgcgc gaggatgtag
     1741 accggacggt cgtagccgag agtccggtag tagtggacca ggaacagacc gtcgaccggg
     1801 ttgaggccgc cgttgtgatt gccgaccagc agcgccggac cgtcgaccgg gatgcggtcg
     1861 aggccgcgga cctcgaggcg gtgataacgg gccagcttgt cgaagcccgc caccgccgag
     1921 gccagcgcgg aggcccgcac cttgccccac ggcgggtcga ccgggcgctc cggctccggc
     1981 gcgaggtcgg ggaccggggt cagcggagca agattgagct ggccggggtg acccgaggcc
     2041 gtcgacatgc tcgacatgct cgacatggcc tgggccgatg tcgcgcgtgc gtccggcgcg
     2101 cgctcgagga cctcagaccc ggggtcgagc cgggagtcga gccgagagtc gagccggccg
     2161 agcgactcga gggggagggc ttccacagcc cgaaggtatg gcttggctag gcggataccc
     2221 gcagtgttgc ataatcacca caccccgtcg accgccgcga aagatttttc gcgccagcgg
     2281 ccgtggcccg ggcaaagccg aggatgcgcg cggcgagggc gtcgggctgc tcaaggaagc
     2341 cgatgtggcc gaaattcggc cattcttcga tctccgcgtg gctgggcagg tgcttgcgaa
     2401 agaattcgag gtgcgtgcgg gggagcacac gttcttcccg tccccacacg agcaaggtgg
     2461 gaatgcgcag cccgctcaac tcgtgcgggg ccagcatctg ctccggggta atactggcga
     2521 gcagctggcg cagctggggg tgcgtgaagc gctggcggat gccgtgggcg agggcgtggc
     2581 ggaggacgcc tgccccgttg gcgaacacgg cgtcgacgaa gtcgagggcc tggcggtggc
     2641 tcgagatgtc gaaggtggcc aggaacttgg cgaactgggc ggcgggcatc ggcgcaccgc
     2701 cgggggacac gagggcgagc gccgcgacgc gctccggacg ggcgatcgcg tagcggatgg
     2761 cggccaggcc gcccatcgag ttgccgacga ggatcgccgg ctcgctcgac tcggcgagca
     2821 cgcggtcgag cacctcgctc agggcggtga ccatcgccgt gtcggtcagg ccgccgacgg
     2881 gcacggcgct gaaaccatgc gccggcatgt cgggggcgat gacccggcgc gtgtgcgggg
     2941 cgaagcgccg gagcaggccg gcgtaacaga tcgcgttggc gctgagcccg tggagcatca
     3001 cgatcggtgg caggttgccg ccacctgtgc tgtcgagcac atgcatgcgc ccgactcggg
     3061 tctcgatgaa gcggctgcgc aaaccctggg cattcaacac acgacgggcg atacgttcgg
     3121 gtacggtcag catgtcggcg ttcgtagtga cgatagcgtg atcgaaaagt ccaagaaacg
     3181 gcggcacata atagtgctgt ccatcacgga tggagcgtgc aaacgcttgc gcgggagggg
     3241 gccggtgtgc tgcgcgccgg cagaagtttc tgcggcgttt ggccccgagt tgaccccgcg
     3301 ggacccgcgc gttcggggat cggtggcccg actgcgcgcc ccgaggagta gg
//
LOCUS       DASWNJ010002045         2683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_351064, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002045 DASWNJ010000000
VERSION     DASWNJ010002045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351064"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VGB85_34035"
     CDS             complement(<1..299)
                     /locus_tag="VGB85_34035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139174.1"
                     /translation="MFPILATAHARYLADLGPGLPDVQAWWQTGAVLVCVMAATAWLT
                     ARWLIRRKKGCAGDCSRCVAGGAATTPGPGACGHTHGGTGIRSDSLRVVRSGGPP"
     gene            complement(311..823)
                     /locus_tag="VGB85_34040"
     CDS             complement(311..823)
                     /locus_tag="VGB85_34040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEY0139175.1"
                     /translation="MHDRRHHPHGTDVETLMGQFHAYLEKKQLKSTRQRDLIAQKFFS
                     TEGHMSIEELLNLSRSENPRIGYATVYRTLKLLTECGLAALRRFGDGQTMYETAGDTE
                     HHDHLICIECQHVLEFQNSDIEMEQERVARSFGFNLVRHRLELYGLCPRARGIKGGWC
                     PSDEAARKGD"
     gene            1022..1264
                     /gene="xseB"
                     /locus_tag="VGB85_34045"
     CDS             1022..1264
                     /gene="xseB"
                     /locus_tag="VGB85_34045"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEY0139176.1"
                     /translation="MDRVLGDLERVVAELEAGDLPLETALKRFEDGVRLARQGGQLLD
                     AVEQRVEMLLEGREGTAPFPGVNDEDEDEEDDDDDV"
     gene            1245..2108
                     /locus_tag="VGB85_34050"
     CDS             1245..2108
                     /locus_tag="VGB85_34050"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY0139177.1"
                     /translation="MTTTTFDLTAYLSDRRRQADEALARALPELGPDDPGKLKEGMQY
                     AVLQGGKRMRPMITIAACEAAGGSIAAALPASCALEMIHAYSLVHDDLPAMDNDLERR
                     GRPTVHVAFGHGHAILIGDALLTRAFEILGRGVAGIAGDRIAAAITYLAHHAGIHGMV
                     GGQALDIEHGQNILSLELLERVHALKTGALYAAGGAMGALCGGANDAQVRDLESWGLK
                     FGIAFQHADDILDDDQQALRAQALARVDELVSECDALAEQFGPRAEPLRALSKWVKDR
                     AHAANAGVKAD"
     gene            complement(2135..2437)
                     /locus_tag="VGB85_34055"
     CDS             complement(2135..2437)
                     /locus_tag="VGB85_34055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139178.1"
                     /translation="MGWATGHIERLLAGETVSFRPRGHSMKGKVDNGALVTVEPVGER
                     VLEVGDIVLCKVGGQQYLHLIKATRGPSYQIGNNRGGINGWVSRAAIFGVLVRVEP"
     gene            2504..>2683
                     /locus_tag="VGB85_34060"
     CDS             2504..>2683
                     /locus_tag="VGB85_34060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139179.1"
                     /translation="MRSVLVIMILAACAPEPTRSEPPTASESAQTPVEAPNAPAATPT
                     AAPAGAVANDPRWHAT"
BASE COUNT          390 a         1007 c          911 g          375 t
ORIGIN      
        1 ttcggggggc cgccggaccg gaccacgcgc aggctgtccg agcggatgcc ggtgccgccg
       61 tgcgtatgcc cgcaggcgcc ggggccgggc gtggtcgcgg cgccgcccgc gacgcaccgg
      121 ctgcaatctc ccgcgcaccc cttcttgcgg cggatcagcc agcgcgcggt cagccacgcg
      181 gtggccgcca tcacgcagac gagcaccgcg ccggtctgcc accaggcctg cacgtccggg
      241 agcccggggc cgaggtccgc gaggtagcga gcgtgagcag tggccaggat gggaaacatg
      301 acggagcggc tcagtcgccc ttgcgcgcgg cctcgtcgga cgggcaccac ccgcccttga
      361 tgccgcgcgc gcggggacac agaccgtata gctcgagtcg gtggcggacc aggttgaagc
      421 cgaagctgcg ggccactcgc tcctgctcca tctcgatgtc ggagttctgg aactcgagca
      481 cgtgctggca ctcgatgcag atcaggtggt cgtggtgttc ggtgtcgccc gcggtctcgt
      541 acatcgtctg gccgtcgccg aagcggcgca gcgcggcgag gccgcactcg gtcagcagct
      601 tgagcgtgcg atacacggtc gcgtacccga tccgcgggtt ctccgagcgg ctgaggttca
      661 agagctcctc gatgctcatg tgtccctcgg tgctgaagaa cttctgagcg atgaggtcgc
      721 gctggcgcgt cgacttgagc tgcttcttct cgaggtaggc gtgaaactga cccatcaggg
      781 tctcgacgtc cgtcccgtgc gggtggtgcc ggcggtcgtg catcggccgt gaccgtaacg
      841 cgaggcccgc cctgcggcaa gcgttgcggg ggtgaaaccg aacccttata gtccgccgcg
      901 catggctacc cccggttcac cccccgcacc ccgacgccga ggcgccggct ctggcacagc
      961 cgctcctgcc gagcccgccg cagccgaggg ccggcccgag gcccccgcgg agagccccgc
     1021 catcgaccgc gtgctcgggg acctcgagcg cgtcgtcgcc gagctcgagg ccggcgacct
     1081 cccgctggag accgccctca agcgcttcga ggacggcgtg cgcctcgccc gacagggcgg
     1141 ccagctgctc gacgcggtcg agcagcgggt ggagatgttg ctcgagggcc gtgagggcac
     1201 cgcgcccttc cccggcgtca acgacgagga cgaggacgaa gaagatgacg acgacgacgt
     1261 ttgacctgac ggcctatctc agcgaccgac gccgccaggc ggacgaggcc ctcgcgcgcg
     1321 cgctgccgga gctcggcccg gacgaccccg gcaagctcaa ggagggcatg cagtacgcgg
     1381 tgctccaggg cggcaagcgc atgcgcccga tgatcacgat cgccgcctgc gaggccgccg
     1441 gcgggtcgat cgcggcggcg ctgccggcca gctgcgcgct ggagatgatc cacgcctact
     1501 cactggtcca cgacgacctc ccggcgatgg ataacgacct cgagcgccgc ggccgtccga
     1561 ccgtgcacgt cgccttcggc cacggccacg cgatcctcat cggcgacgcc ctgctgaccc
     1621 gcgccttcga gatcctcggg cgcggggtcg ccgggatcgc cggcgaccgc atcgccgccg
     1681 cgatcaccta cctcgcccat cacgcgggca tccacggcat ggtcggcggt caggccctcg
     1741 acatcgagca cggccagaac atcctctcgc tcgagctgct cgagcgcgtg cacgccctca
     1801 agaccggcgc cctgtatgcg gccggcggcg cgatgggggc cctgtgcggc ggcgcgaacg
     1861 atgcccaggt ccgtgacctc gagtcctggg gcctcaagtt cggcatcgcc ttccagcacg
     1921 ccgacgacat cctcgacgac gaccagcagg ccctgcgcgc ccaggcgctc gcccgcgtcg
     1981 atgagctcgt gtccgagtgc gacgccctcg ccgagcagtt cggcccccgc gcggagcccc
     2041 tgcgcgcgct gtcgaagtgg gtcaaggacc gggcccacgc cgccaacgcc ggcgtcaagg
     2101 ccgactgagc ctgtcctccg ggacaggtgc ctcgttacgg ctcgacgcgc acgagcaccc
     2161 cgaagatcgc cgcgcgggac acccacccgt tgatcccgcc gcggttgttg ccgatctggt
     2221 agctcggccc gcgcgtcgct ttgattaaat gcaggtactg ctggccgccg accttgcaga
     2281 gaacgatgtc gccgacctcg agcacgcgct cgccgaccgg ctcgaccgtc accagcgcgc
     2341 cgttgtcgac cttgcccttc atgctgtgcc cgcgcggacg aaagctgacc gtctcgcccg
     2401 cgagcagccg ctcgatgtgt cccgtcgccc agcccatgtc aggatcatgt aaaagcgtcg
     2461 ccgcgtcgct gacaagcccg cagcaccgat ctatcgtcgt gtgatgcgct ccgtgctcgt
     2521 aatcatgatc ctcgccgcgt gcgcccccga gcccacgcgc agcgagccgc ccaccgccag
     2581 cgagtccgcg cagacgcccg tcgaggcccc gaacgcgcct gccgcgacac ctaccgcggc
     2641 ccctgcgggc gcggtcgcca acgacccgcg ctggcacgcg acc
//
LOCUS       DASWNJ010002046         3131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_351118, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002046 DASWNJ010000000
VERSION     DASWNJ010002046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351118"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1567)
                     /locus_tag="VGB85_34065"
     CDS             complement(<1..1567)
                     /locus_tag="VGB85_34065"
                     /inference="COORDINATES: protein motif:HMM:NF024486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HEY0139180.1"
                     /translation="MSPTIHLQRLVIEFELEQLPRPVVELLGSFAGPDTPQDDQLIEY
                     LDLILPHGAPTTLYISNISTHPTPAHFERNLLLRPACEGPLADSYGRGNLTLALSAYR
                     RKGKLVVSRLEITPHVPLRGFEARISAPVFWRSASDQYMTSEDFEALGGLHAHRLDTR
                     NRLQPWRRYLEWKEDLVKRAQIDIPYLAWRRENATCFAFLVRSTDVPDRPPTNVEFDA
                     TVPEPEDDEPDLESVAPRRRKAPRPPEPIRLGMVDRVQRIDPRDSQQTKPWGKMAGPG
                     QLELILRVDEDQARKLDKAELPAPGRLLSSIAGELKPLRLQVAGISRLETSQGFCPRL
                     IDFLFDARNAGAPVLPAELAPVAGGRALNPGQQEAVRKALAAPDLCLIQGPPGTGKTT
                     VIAELCLRVALQGGRVLVASQTNLAVDNALSRLADRPAIRRLRLGAAGKVDEEFRDFL
                     ADNVIPRWFRTIADQCHRRMDAAEALRVAYHARDRALAELRRALQAEAEAGRAERDAV
                     LRANNLEAWLVAAM"
     gene            complement(1593..>3131)
                     /locus_tag="VGB85_34070"
     CDS             complement(1593..>3131)
                     /locus_tag="VGB85_34070"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139181.1"
                     /translation="EGAYPFSSVRPERLQELIHTMLEREILYEADGLLSLGQRGEKLY
                     GRRHFFELYAVFTAPPVMRVMHGREDVGFIQATFVSMHDRNTGPLCFRLAGRAWEVGP
                     IDWAKSVLHVHPAEHGRVPSWLGLPGTLSRELCQAMMDVLAREGDEGAWLSPTVAKEL
                     GMLRESYAGLLAQGTAPLEELPDGVLWHTFAGGAVNRLLAAGLEQKSGKRWVAGNLSV
                     RCKDLPLAFAKQAVKELAGLNWEQTAADAARGMARGMVSKFQPCLPEAAEDRLLAEKL
                     LDLAGTLQFLGSVALGSIRAAAQPTGTRLADGENKPLGLELRFPEAPASAATPKNAVH
                     WVDTPASLRAACAELQAAEIVALDVETALDFGTLLLVQIAIRRCTFLIDPFAVGDLGP
                     LRAVLDAPSPRKVIHNARFERRILGRVGIPLQGVYDTMEASRRIHGAKTLGGHSLAIV
                     SERELGLSLDKSEQTSDWSRRPLGADQLRYAALDAEILLALYDRLNHGAGVDPQELQL
                     SMLS"
BASE COUNT          483 a          952 c         1194 g          502 t
ORIGIN      
        1 gcatggcagc gacgagccac gcttcgaggt tatttgcgcg gagtacggcg tcgcgctcgg
       61 cacggccggc ctctgcctca gcctggaggg ccctgcgcag ctcggcgagg gctcggtccc
      121 tcgcatggta cgcgacccgc agagcctcgg cggcgtccat acgacggtgg cattgatcgg
      181 cgatggtccg aaaccaccgc ggaatcacgt tgtcggcgag gaagtcgcgg aattcctcgt
      241 cgactttgcc ggcagcgccg agtcggaggc gacggatggc cgggcgatcg gcgaggcgcg
      301 acagggcgtt atcgacggcg aggttggtct gggaggcgac cagcactcgg ccaccttgca
      361 gcgcgacccg cagacatagc tcggcgataa cggtcgtctt gccggtgccc ggcgggccct
      421 ggatgaggca aaggtcagga gccgcgagcg ccttgcgcac ggcctcctgc tgcccggggt
      481 tgagcgcgcg accaccggcc accggcgcga gctcggccgg tagcacggga gcgccagcat
      541 tgcgagcgtc gaagagaaag tcgatgagcc gcggacagaa cccctggctg gtctcgaggc
      601 gggagatgcc ggcgacttgc aggcgtagcg gcttgagctc gccggcgatg ctcgagagca
      661 ggcgccctgg tgccggcagt tcagccttgt cgagcttgcg cgcctggtcc tcgtcgacgc
      721 ggaggatcag ttccagttgt ccggggcccg ccatcttgcc ccacggcttc gtctgctggg
      781 agtctcgcgg gtcgatgcgc tggacacgat cgaccattcc gaggcggatc ggttcaggcg
      841 ggcgcggagc tttccggcgg cgcggcgcga cggactcgag gtccggctca tcatcctctg
      901 gctccgggac ggtcgcgtcg aattctacat tggtaggagg gcggtcgggg acgtctgtgc
      961 tgcgaaccag gaaggcgaag caagtagcgt tctctcgtcg ccatgcgagg taaggaatgt
     1021 cgatctgggc ccgcttgacg aggtcttctt tccactcgag gtacctgcgc cacggctgca
     1081 gccggtttct ggtgtcgagg cggtgcgcgt gcagcccacc gagggcctcg aaatcttccg
     1141 acgtcatgta ctggtcggag gccgatcgcc agaacaccgg ggccgagatc cgggcctcga
     1201 agccacgaag tgggacgtgc ggcgtgatct cgaggcgcga gacgacgagc ttgcccttgc
     1261 gccgataggc cgagagcgcg agcgtaaggt tgccgcggcc atatgaatca gcgagtgggc
     1321 cctcgcacgc ggggcgcagc aggaggttac gctcaaagtg agcgggggtc ggatgggtgc
     1381 tgatgttgga tatgtacaga gtggtcgggg cgccgtgtgg caagatgaga tcgaggtact
     1441 cgatcagctg gtcatcctgc ggggtgtcgg ggccggcgaa cgagccgagc agctcaacga
     1501 cgggccgcgg caactgttcg agctcgaact cgatgaccag gcgctggaga tggatcgtgg
     1561 gggacattcg tcggttgcgg agcgagcgga gctcacgaga gcatggacag ctgtagctcc
     1621 tgcgggtcga cgccagcgcc gtggtttagg cggtcgtaca gggccagcag gatctcggca
     1681 tccagggcgg cgtagcggag ctggtcggcg ccgagggggc ggcggctcca gtcggaggtc
     1741 tgctcggatt tgtcgagaga gagcccgagc tcgcgttcgc tgacgatcgc caggctgtga
     1801 ccgccgaggg tcttcgcgcc atggatgcgt cgggaggctt ccatggtgtc gtacacgccc
     1861 tgtagtggga tgccgacgcg gccgaggatc cggcgctcga agcgggcgtt gtggatgacc
     1921 ttgcgcgggc ttggtgcgtc gaggacggcc cggagcgggc ccaggtcgcc gacggcgaag
     1981 gggtcgatca ggaaagtgca gcgccggatg gcgatctgga cgaggaggag cgtgccgaag
     2041 tcgagggcgg tctcgacgtc gagggcgacg atctcggcgg cttgaagctc ggcgcacgcg
     2101 gcccggaggc tggcgggggt gtcgacccaa tggaccgcgt tcttgggcgt ggcggcgctt
     2161 gcaggggctt cggggaagcg gagctcgagg ccgaggggtt tgttttcgcc gtccgcgagg
     2221 cgcgtgccgg ttggttgcgc tgcggcacga atactgccca gcgcgacgct gccgaggaat
     2281 tgcagcgtgc ccgccaggtc gagtagcttc tccgcgagca ggcggtcctc cgcggcctcg
     2341 ggcaggcagg gctggaattt gctgaccatg ccgcgggcca tgccgcgcgc ggcgtcggcg
     2401 gcggtctgct cccagttcag cccggcgagc tccttcacgg cctgtttggc gaaggccagc
     2461 gggaggtcct tgcaacgcac cgagaggttg ccggccaccc agcgcttgcc ggacttctgc
     2521 tcgagcccgg ccgcaagcag gcggttgacg gcgcccccgg cgaaggtatg ccacaggacg
     2581 ccgtcgggga gctcctcgag gggagccgtg ccctgggcga gcaggcccgc gtaggactcg
     2641 cgcagcatgc ccagttcctt ggcgaccgtg ggggagagcc atgcgccctc gtcgccctcg
     2701 cgggcgagca cgtccatcat cgcttggcac agctctctcg acagcgtgcc cgggagcccg
     2761 agccagctcg ggacccggcc gtgctcggcg gggtggacgt ggagcacgct cttggcccag
     2821 tcgatcgggc cgacctccca ggcgcggccg gccaggcgga agcacagcgg accggtgttg
     2881 cggtcgtgca tcgatacgaa ggtcgcctgg atgaagccga cgtcttctcg gccgtgcatg
     2941 acgcgcatca ccgggggggc ggtgaacacg gcgtagagct cgaagaagtg ccggcgccca
     3001 tacagctttt caccgcgctg gccgagcgag agcaggccgt cggcctcgta caggatctcg
     3061 cgctcgagca tggtatggat cagctcttgc agacgctcgg gacgcacgct cgaaaatgga
     3121 tacgcgccct c
//
LOCUS       DASWNJ010002047         4571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_423035, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002047 DASWNJ010000000
VERSION     DASWNJ010002047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423035"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..978)
                     /locus_tag="VGB85_34075"
     CDS             complement(184..978)
                     /locus_tag="VGB85_34075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139182.1"
                     /translation="MRLSPLLLVLAVACADREVPADDSTAEDTTADVGTTGPTSDPTG
                     PVTDASTGPTTGELDNPSGFCSPAQDPGACPADYICCSDDPATTKARLPNYFNGKNDD
                     KYGVPIFSGANNALSFSGQCVDVGEFPSPFEGGCPVPCDPTWDPADRDALCGVGALCC
                     PFRQVDPIKDCVLDPVTNKWRAVHGTDIGKLTTWGPAHTTNQDPTGASCLSFAGGAMP
                     DKAVLADCYEQLTVADRRGFCYKTCPCYEDLCDQKNPGYVPRCGGP"
     gene            1120..1485
                     /gene="queD"
                     /locus_tag="VGB85_34080"
     CDS             1120..1485
                     /gene="queD"
                     /locus_tag="VGB85_34080"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEY0139183.1"
                     /translation="MEIFKEFRFEAAHRLPNVPPEHKCARLHGHSFVVQVHVRGTVGE
                     RSGWIVDFADIAVAWAPLHAVLDHYFLNEIAGLENPTSEILAQWIWRKLLPTLPGLSQ
                     IQVRETCTSGCVYRGEDEG"
     gene            1642..2226
                     /locus_tag="VGB85_34085"
     CDS             1642..2226
                     /locus_tag="VGB85_34085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEY0139184.1"
                     /translation="MIGLRSVALDAEHVVEVGVMPAELVPDGAGFAALWDLHPGDFHE
                     ILMHGRKVKTPRWQQAYGADYRYTGNVNKAEPLTPQMQVWLAWARACFDARLNGLLLN
                     WYDGTVGHYIGKHRDSEIQRVEGSAIVTLSFGEARVFRMRPWRGTGFIDIPVENGSVL
                     VIPWATNRAFTHEVPASAAARGRRVSVTMRAFHG"
     gene            2258..2626
                     /locus_tag="VGB85_34090"
     CDS             2258..2626
                     /locus_tag="VGB85_34090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139185.1"
                     /translation="MTHWFECAQPGDARAVLGELRSAGKRQRWIDPERWALRAGMLSP
                     TLIQAESVTKWGADVSLGEVGRKVGNVRNWAYTGEVSLEDGADHNPVIHELADGPFSR
                     SIYIPDTKPVVLTRLRWPLT"
     gene            2932..3642
                     /locus_tag="VGB85_34095"
     CDS             2932..3642
                     /locus_tag="VGB85_34095"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEY0139186.1"
                     /translation="MSTGTSTGETTGGSSGAPGGDGEGGSSSSTGAGPVCGDGIVDAL
                     EECDDGNLDETDRCTSSCEKIRLVFITSLIFQGNINGLGGADAYCKSSASKAMKEDPT
                     SSRITDPGNFKALLPISTQTVFERHFHGKGPYQLVNGLRVSDSFETLFSEPHHNPINV
                     DERSQSQHALVWTGMDVDGTSYPGTDFCQNWTSTKGSSTWGDADSVDGWWLFVDVAVD
                     NPSTDCGGGFALYCLEQE"
     gene            3645..>4571
                     /locus_tag="VGB85_34100"
     CDS             3645..>4571
                     /locus_tag="VGB85_34100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139187.1"
                     /translation="MKISHFSHRCLPNLLILLSAACPASGPEVGLEPGSTGSTSTGEA
                     TPDTPTGAGPSSSGDAASSSSGEPDTSSSGFADPGELCGDGIPGLGEQCDDGEANDPY
                     GPCTDTCQVNVCGDGKVLVGTEECDEGPDNVDTGYCRMDCKLGVCGDGFLFAGLEECD
                     AGVGSDAYGKCDASCTVNRCGDGELDPGFEECDEGPNNGAGGGEGGKVGCDLDCGLAG
                     RRLFLSSQLFDGDLGTRAGADLACQIMAKAAHFKNPERFRALLADAEVGPADFVAEDP
                     EERPFILPSGLIVAASYAALIQGGPGDGITMTE"
BASE COUNT          734 a         1420 c         1692 g          725 t
ORIGIN      
        1 tcgggctcgt gctcgagggc ggctacgacc tcgatggcct ggccgacagc gcgtacgcct
       61 gcgtcgaggt gctcgccggg gcgacgcccc cggtggccgg cccggcgagc gcggccggcg
      121 aggcggcgct gcgccggacg ctggcgtcgc agcggccgca ctggcggctg tgacggccag
      181 cgctcagggg ccgccgcagc gcggcacata gcccgggttc ttctggtcgc acaggtcctc
      241 gtagcagggg catgtcttat aacaaaagcc gcgccggtcg gcgacggtga gctgctcgta
      301 gcaatctgcc agcacagcct tgtcaggcat cgcgccgccg gcaaaggaca ggcacgacgc
      361 gccggtgggg tcctggttgg tggtatgggc gggaccccag gtcgtcagct tgccgatatc
      421 ggtgccgtgc acggcccgcc acttgttggt caccggatcg agcacgcagt ctttgatcgg
      481 atcgacctgg cgaaacgggc agcacagcgc accgacaccg cagagcgcgt cgcgatcggc
      541 ggggtcccag gtcgggtcgc aggggaccgg gcagccgccc tcgaagggcg aggggaactc
      601 gccgacgtcg acacattgcc ccgagaaact aagagcattg ttggcgccgg agaagatcgg
      661 gacgccatac ttgtcgtcgt tcttgccgtt gaaataattc ggcagacgag ccttcgtcgt
      721 cgccgggtcg tccgagcaac agatgtagtc cgcagggcac gctccggggt cctgcgccgg
      781 ggaacaaaaa ccggaggggt tgtcgagctc gccggtcgtc gggcccgtgc tcgcgtcggt
      841 gacggggccc gtcgggtcgg aggtcgggcc ggtcgtgccg acgtcggcgg tcgtgtcctc
      901 ggcggtcgag tcgtcggcag ggacttcacg gtcggcacag gcgacggcaa ggacgagcag
      961 gagcggcgag aggcgcatca cccgactttt cctgcgtccg gaccgatgcg caagcgggct
     1021 cgtacgcggc agcgcgtcga ccgcgccgcc tggcccatcc gccgtgactt gaagcagctc
     1081 ggcctgcgcg cggttatcgg ctaccttccc gcgcccgcca tggagatctt caaagagttc
     1141 cgcttcgagg ccgcccaccg cctgccgaac gtgcccccgg agcacaagtg cgcccgcctg
     1201 cacgggcact cgttcgtggt ccaggtgcac gtgcgcggca cggtcggtga gcgcagcgga
     1261 tggatcgtcg acttcgccga catcgccgtc gcgtgggcgc cgttgcacgc ggtgctcgac
     1321 cactattttt taaatgagat cgccgggctc gagaacccga ccagcgagat cctcgcgcag
     1381 tggatctggc gcaagttgct gccgacgcta ccggggctgt cgcagatcca ggtgcgcgag
     1441 acgtgcacgt cgggctgcgt ctatcgcggc gaagacgagg gctgaaaaga tcggcgaggc
     1501 gccgggccgg tgttgtaagg tcgctggcat ggcctggctc gcgcgcgtcg agatcgtggt
     1561 cgcgctcgtg ggcgccgcgg aggcccgcgc gcacggagag cttcatgagc gtactttgat
     1621 ccggcggcgg agggcggcgc ggtgatcggc ctgcgtagcg tcgcgctcga cgcggagcac
     1681 gtcgtcgagg tcggggtgat gccggcggaa ctggtgccag atggggcagg cttcgcggcg
     1741 ctgtgggatc tgcaccccgg agattttcat gagatcttga tgcacgggcg caaggtcaag
     1801 acgccgcgct ggcagcaggc ctacggcgcg gactatcgct atacggggaa tgtcaataag
     1861 gcggagccgc tgacgccgca gatgcaggtg tggctcgcgt gggcgcgtgc gtgttttgat
     1921 gcgcggctca atgggctgct gctcaactgg tacgacggga cggtggggca ctacattggc
     1981 aagcaccgcg acagcgagat ccagcgggtc gagggatctg cgatcgtgac gctgtcgttc
     2041 ggggaggcgc gcgtgtttcg tatgcgtccg tggcggggga ccggcttcat cgatattccg
     2101 gtggagaatg gttctgtgct ggtgatcccc tgggcgacca accgcgcgtt cacccacgag
     2161 gtccccgcct ccgccgcggc gcgcggacgc cgggtgtcgg tgacgatgcg ggcgttccac
     2221 ggctgacggg agcgacgcga tgggcaactg gtgcacgatg actcactggt tcgagtgcgc
     2281 gcagccgggc gacgcgcggg ccgtgctcgg cgagctgcgg tcggctggca agcgccagcg
     2341 ctggatcgac ccggagcgct gggcgctgcg cgctggcatg ctctcgccga cgctgatcca
     2401 ggccgagagt gtgacgaagt ggggcgcgga cgtcagcctc ggggaggtgg ggcgaaaggt
     2461 cgggaatgtt cgcaactggg catataccgg cgaggtgtcg ctcgaggacg gcgcggacca
     2521 caacccggtg atccacgagc tcgcggatgg ccccttttct cgatctatct acattcccga
     2581 cacgaaaccg gtcgtgctca cgcgcctgcg ctggccgctg acgtgatccg gcgacggaca
     2641 aatctaccgg atctactcca gaggcagatc gtgtattttg aattacaaat attactgagc
     2701 agattttctt gacaggggcc gcggtgagtc gccaatctcc ggttcatggg aacgacgtgg
     2761 cgctgcctcg catgtgtgct cgtgaccggc tgcgggccgc aacgcgactg ggaggccttc
     2821 caagcggcct ttgaaagcgc ctcgcggggg agctcgagct cgtcgggacc ccaggcagac
     2881 accggctccg ggacctcggg gagcgagacc ggaggcgagt ccagcgaggg tatgagcacc
     2941 gggacttcca cgggggaaac cacgggcggg agctcggggg ctccgggtgg cgacggcgag
     3001 ggcgggtcgt cgtcgtcgac gggggccggg ccggtctgcg gcgacggtat cgtcgatgct
     3061 ctggaggagt gcgacgacgg gaacctcgac gagacggatc ggtgcacgag cagctgcgag
     3121 aagatccgcc tcgtgttcat caccagcctc atctttcagg gcaacatcaa cggtctgggg
     3181 ggggcggacg catactgtaa gtcgtcggcg tccaaggcga tgaaggagga cccgacgtcg
     3241 tcgcggatca cggacccggg caacttcaag gcgctgctgc cgatctcgac gcagacggtg
     3301 ttcgagcggc actttcacgg caagggcccc tatcagctcg tcaacgggct gcgcgtgagc
     3361 gacagctttg aaacgctgtt cagcgagccg caccacaacc cgatcaacgt cgacgagcgc
     3421 agccagagcc agcacgcgct ggtgtggacg gggatggacg tcgacggcac gagctacccc
     3481 ggcaccgatt tttgccaaaa ctggaccagc accaagggct cgtcgacctg gggggacgcc
     3541 gactcggtcg acggctggtg gctgttcgtc gacgtcgcgg tcgacaaccc cagcaccgac
     3601 tgcggcggcg ggttcgcgct gtattgcctc gaacaggagt gaccatgaag atctctcatt
     3661 tttcccaccg ttgtttgcca aacctactaa ttctgctttc ggctgcctgc cccgcgagcg
     3721 gccccgaggt cgggctggaa ccgggctcga ccgggagcac atcgaccggg gaggcgacgc
     3781 ccgacactcc gaccggcgct ggcccgtcga gctcggggga cgccgcttcg agcagctcgg
     3841 gcgagccgga taccagcagc agcggcttcg ccgaccccgg cgagctgtgc ggggacggca
     3901 tcccgggtct cggcgagcaa tgcgacgacg gtgaggccaa cgacccctac gggccctgca
     3961 cggacacctg ccaggtcaat gtctgtgggg acggaaaagt cctggtcggg accgaggagt
     4021 gcgacgaggg ccccgacaac gtcgacaccg gctattgccg gatggactgc aagctcggcg
     4081 tgtgcggcga cggctttttg ttcgcgggcc tcgaggagtg cgatgcaggg gtcggcagtg
     4141 atgcgtacgg caagtgcgac gcgagctgca cggtcaaccg ctgcggcgac ggcgagctcg
     4201 acccgggctt cgaggagtgc gacgagggcc cgaacaacgg cgccggcggt ggggagggcg
     4261 gcaaggtcgg ctgcgacctg gattgcgggc tcgccgggcg ccggctgttt ctgtcctcgc
     4321 agctgttcga cggggatctc gggactcgcg cgggggccga tctggcgtgc cagatcatgg
     4381 ccaaggcggc ccacttcaag aacccggagc gcttccgggc gctgctcgcc gacgcggagg
     4441 tcggtcctgc ggacttcgtg gcggaggacc ccgaggagcg gccgttcatc ctcccgagcg
     4501 ggctgatcgt cgcggcgagc tacgccgcgc tgatccaggg cggacccggt gacggcatca
     4561 ccatgaccga g
//
LOCUS       DASWNJ010002048         1987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_351345, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002048 DASWNJ010000000
VERSION     DASWNJ010002048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351345"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="VGB85_34105"
     CDS             complement(<1..913)
                     /locus_tag="VGB85_34105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0139188.1"
                     /translation="MSSAYNTRILVVDDEEVVRDSIRESLRPPPRKDPALASAAAALF
                     GDEPAAAGSDTAAAELLVFEVDEAATGPAGLARVEAAIAAGRPYAVIFLDMRMPGWDG
                     LRTAQRIREIDTRAELIFVTAYSDASLDEVIAKAGANVGYHCKPFAPEEIRQIATKGV
                     HDWHKVRGLEALIDVIGGLRGSEREVNTLLHNILGQVAAMVGSDAALLGRTGPDGAFD
                     PLFGVGAWQDPRRAGPVLAIVGALANRGPAGAVTLRANLAIMHLESYRIAAAVRETSS
                     FDAEKVYLLELFLASAGQALENARLHER"
     gene            complement(910..>1987)
                     /locus_tag="VGB85_34110"
     CDS             complement(910..>1987)
                     /locus_tag="VGB85_34110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0139189.1"
                     /translation="FHALVDVAPDLRHAVATYAPPSGPVVRGRVLHVAAGALRRHYSL
                     DVIRIDRDTYMAALADVTEAVDNETLAREALQLTAVQEGRFEVLTSVLHDIGNGITGL
                     GTRTAMLAAGPAWEEVEQLDRLAQFVRQQRAALEPALGADRSGALVSFVDAVTARLRE
                     RMTQWRETLSFFVASIAHVQEIMNIQRQFIRGGEARRPLAVTELLDDALALQRAVLQK
                     RGVVVSLDVPPRVPRIEADRTRMMQVFVNVLKNVVEAFDAVEPASAATRTLQLSVRTV
                     ADDHLEIAFTDNACGFAAPNGEFPIARGATSKPGGSGLGLYASAQIVASHHGSLTLRS
                     PGPGLGATCIVTLPLRQPPEPAPP"
BASE COUNT          255 a          676 c          779 g          277 t
ORIGIN      
        1 cgcgctcgtg cagccgggcg ttctccagcg cctggccggc gctggcgagg aacagctcga
       61 gcaggtagac cttctcggcg tcgaaggagc tcgtctcgcg caccgccgcg gcgatgcggt
      121 acgactcgag gtgcatgatc gccaggttgg cgcgcagcgt gacggcgccg gcggggccac
      181 ggttggccag cgcgccgacg atcgcgagca ccggccccgc gcgcctgggg tcttgccacg
      241 cgccgacgcc gaacagcggg tcgaaggcgc cgtcgggccc ggtgcgcccg agcagcgccg
      301 cgtccgagcc gaccatggcc gcgacctggc cgaggatgtt gtggagcagg gtgttgacct
      361 cgcgctcgct gccacgcagc ccgccgatga cgtcgatcag cgcctcgagg ccgcggacct
      421 tgtgccagtc gtgcacgccc ttggtggcga tctggcggat ctcctcgggc gcgaagggct
      481 tgcagtggta gccgacgttg gcgcccgcct tggcgatcac ctcgtcgagc gaggcgtcgc
      541 tgtaggcggt cacgaagatc agctcggccc gggtgtcgat ctcgcggatc cgctgggcgg
      601 tgcgcaggcc gtcccagccg ggcatgcgca tgtcgaggaa gatgacggcg tacgggcggc
      661 ccgccgcgat ggcggcctcg accctggcca ggcccgcggg gccggtcgcc gcctcgtcga
      721 cctcgaacac caggagctcg gcggcggccg tgtccgaccc ggccgcggcg ggttcgtcac
      781 cgaacagcgc cgcggcggcg gaggcgagcg cggggtcctt gcgcggcggc gggcggagcg
      841 actcacggat gctgtcgcgc acgacctcct cgtcgtcgac gaccaggatg cgggtgttgt
      901 atgcggagct catggtggtg cgggctcggg cggctggcgg agcgggaggg tgacgatgca
      961 ggtcgcgccc aggccggggc cggggctgcg cagggtgagg ctgccgtggt gcgaggcgac
     1021 gatctgggcc gaggcgtaca ggccgagccc gctcccaccg ggcttggagg tcgccccccg
     1081 cgcgatcgga aactcgccgt tcggcgcggc gaagccacag gcgttgtcgg tgaacgcgat
     1141 ctcgaggtgg tcgtccgcga ctgtccgtac ggacagctgc agcgtgcggg tcgctgcgct
     1201 ggcgggctcg accgcgtcga acgcctcgac gacgttcttg agcacgttga cgaacacctg
     1261 catcatccgg gtgcgatcgg cctcgatgcg cggcacccgg ggcgggacgt cgaggctgac
     1321 caccacgccg cgcttttgca gcaccgcgcg ctggagcgcg agcgcgtcgt ccagcagctc
     1381 ggtcaccgcg agcgggcggc gagcctcgcc gccgcggatg aactggcgct ggatgttcat
     1441 gatctcctgg acgtgcgcga tcgaggcgac gaaaaagctc agcgtctcgc gccactgcgt
     1501 catgcgttcg cgcaggcggg ccgtcaccgc gtcgacgaag ctgacgagcg cgccgctcct
     1561 gtccgcgccg agcgcgggct cgagcgcggc ccgttgctgt cgcacaaatt gggcgagccg
     1621 gtcgagctgt tcgacctcct cccacgccgg cccggcggcg agcatggcgg tgcgcgtgcc
     1681 gagcccggtg atgccgttgc cgatgtcgtg gaggacgctg gtgagcacct cgaagcggcc
     1741 ctcctgcacg gcggtcagct gcagcgcctc gcgggcgagg gtctcgttgt cgacggcctc
     1801 ggtgacgtcg gcgagcgccg ccatgtacgt gtcgcggtcg atgcggatca cgtcgagcga
     1861 gtagtgccgg cgcagcgcgc ccgcggcgac gtgcaggacc cggccgcgca cgacgggccc
     1921 ggacggcggc gcgtaggtgg cgacggcgtg ccgcaggtcg ggcgcgacgt cgacgagcgc
     1981 gtggaac
//
LOCUS       DASWNJ010002049         2135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_351353, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002049 DASWNJ010000000
VERSION     DASWNJ010002049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351353"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /locus_tag="VGB85_34115"
     CDS             <1..1226
                     /locus_tag="VGB85_34115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139190.1"
                     /translation="IDNSGSMGEEQTNLAANFPYLIQKIRDLKDKNMELVNADVNIMV
                     TTTDFGHPLCTNFQKPDYTPAKGAPINTACTTRLERFNGIGSGVMIPQACTNGCDANA
                     PAAPMGPFIHFTPSSNNIMNANPDPMRDPVADALSCIGPQGIDGCGMEATLETMLQAL
                     DPSKAWNSSNEPFLRQGAVLAIIVVTDEEDCATKDYRHFDPGNVDDPMFNQYWEVNPT
                     SGKKDNPTSATCWNAGVTCFDNNADGIYEGCMSEDKGVLQPIERYTNYLKKVLIEDKN
                     KQVVMLGILGVPPVTARNDKPPFEPTDGGVFDLVYRDWLPSDILPGDTKDAPYKQYEF
                     GIGPGCSNAATGQAVPPVRVKEVCESLNVPDDPNTPENDPKLRCCIESICDTDFSNAI
                     NCLAGILQNELAPAG"
     gene            complement(1359..1952)
                     /locus_tag="VGB85_34120"
     CDS             complement(1359..1952)
                     /locus_tag="VGB85_34120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139191.1"
                     /translation="MNNLEDRDDRVEDTWAKSLRDVLCDVIKTLPSHVTLGELVDATR
                     SNPQLAPVLDHFTVQELIDLVKDRPRNEPSDLPVRAAPRSGSEPRPEVQFDEEGNPLL
                     DLDLDSGPAVIRRRADVPDGDVRLLSALSKQKAGRRENELLQTTGLASDQVRLILRNL
                     RTRGFIHVEGSGIKRRIKITRHGTAYLRKFEPNALSL"
BASE COUNT          400 a          703 c          703 g          329 t
ORIGIN      
        1 tgatcgacaa ctcgggctcg atgggtgagg agcagaccaa cctcgccgcc aacttcccgt
       61 atctgatcca gaagatccgc gatctgaagg acaagaacat ggagctggtc aacgccgacg
      121 tcaacatcat ggtgacgacg acggacttcg ggcatccgct gtgcacgaac ttccagaagc
      181 cggactacac gccggccaag ggcgcgccga tcaacaccgc ctgcaccacc cgcctcgagc
      241 gcttcaacgg catcggtagc ggcgtcatga tcccccaggc ctgcaccaac gggtgtgatg
      301 ccaacgcccc ggccgcgccg atgggtccgt tcatccactt cacgccgagc agcaacaaca
      361 tcatgaacgc caacccggac ccgatgcggg acccggtcgc ggacgcgctg tcgtgcatcg
      421 gcccgcaggg catcgacggc tgcggcatgg aggcgaccct cgagacgatg ctgcaggcgc
      481 tcgacccgag caaggcgtgg aactcgagca acgagccgtt cctgcgccag ggcgcggtgc
      541 tcgcgatcat cgtggtgacc gacgaggagg actgcgcgac caaggactac cggcacttcg
      601 acccgggcaa cgtcgacgac ccgatgttca accagtactg ggaggtcaac ccgaccagcg
      661 gcaaaaagga caacccgacc agcgcgacct gctggaacgc cggtgtgacg tgcttcgaca
      721 acaacgcgga cggcatctac gagggctgca tgtcggagga caagggcgtg ttgcagccga
      781 tcgagcgcta cacgaactac ctcaagaagg tgctgatcga ggacaagaac aagcaggtcg
      841 tcatgctcgg catcctcggc gtgccgccgg tgaccgcgcg taatgacaag ccgcccttcg
      901 agccgaccga cggcggcgtg ttcgacctcg tgtaccgcga ctggctgccc tcggacatcc
      961 tgccgggcga caccaaggac gccccgtaca agcagtacga gttcggcatc gggccgggct
     1021 gcagcaacgc ggcgaccggt caggcggtcc cgccggtgcg cgtgaaggag gtctgcgaga
     1081 gcctcaacgt gccggacgac ccgaacaccc cggagaacga ccccaagctg cgctgctgca
     1141 tcgagtcgat ctgcgacacc gacttctcga acgcgatcaa ctgcctcgcc ggcatcctcc
     1201 agaacgagct ggccccggcc ggctgatccg gccgcgaccg ggcccgctgc accgcgggcc
     1261 cgccatacga gacacggggc cctgcgatcg caggaccccg tggtcgttta aaaccggcgc
     1321 cggttcaagt cggcgccggt tcaagccgtg cgcgtcgctc acagcgacag cgcgttgggc
     1381 tcgaacttgc ggaggtacgc ggtgccgtgg cgggtgatct tgatgcgccg cttgatgccg
     1441 ctgccctcga cgtggataaa gccgcgggtc cgcaggttgc gcaggatcag gcggacctgg
     1501 tccgacgcga gcccggtcgt ctgcagcagc tcgttctccc ggcgaccggc cttttgcttg
     1561 ctcagcgcgg agagcaggcg aacgtcgccg tcggggacgt cggcgcggcg gcggatgacg
     1621 gcgggtccac tgtcgaggtc gaggtcgagc agggggttcc cctcctcgtc gaactggacc
     1681 tccggccggg gctcggaccc ggatctcggc gcggcgcgga ccggcaggtc ggacggctcg
     1741 ttcctcggcc ggtctttgac caggtcgatc agctcttgca ccgtgaagtg gtcgagcacc
     1801 ggcgcgagct gggggttgct gcgcgtcgca tcgacgagct cgccgagcgt cacatgactc
     1861 ggcagggttt tgatcacatc acacaggaca tcacgcaggc tcttggccca ggtgtcctcg
     1921 acccggtcgt cccggtcttc cagattgttc acagatgcac ctcagtgcag gttgtcatgg
     1981 gccggaggcc gctttcagtg cgagcgcgtc aggcccggag agatcgacga tatctacatt
     2041 ggcgatgtac aggcgagctc ggcgctaaat aacgggaccc gatgatccgg acgattcgga
     2101 tagttcgaat tccaggtgtt tgagccgagt gaatc
//
LOCUS       DASWNJ010002050         8216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_423173, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002050 DASWNJ010000000
VERSION     DASWNJ010002050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 8216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8216
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423173"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VGB85_34125"
     CDS             complement(<1..319)
                     /locus_tag="VGB85_34125"
                     /inference="COORDINATES: protein motif:HMM:NF015497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HEY0139192.1"
                     /translation="MPPDSLLRARWMLALCALLAPALALADTSATLATAINIPKGPAS
                     IEGFGRAYEVSPASGLPSLNYPLEVPPGRAGHAPQLALRYHAGGGAGVLGLGWSLDLP
                     VIER"
     gene            complement(469..4353)
                     /locus_tag="VGB85_34130"
     CDS             complement(469..4353)
                     /locus_tag="VGB85_34130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139193.1"
                     /translation="MKRTPMFQAAASALLFSLSCAPDDVAQDGSTSTGTTPATGAATA
                     DDSGPEPEPPPPANPWPDGSPCQVDADCDAGDLCELRICVAGCEDAAGCEPGQVCDLH
                     GRCHSEAGDATALHFAGTPTLTERQTVLAVGETRAHTVLRNDGTEGLTYRLAAANPAL
                     TLDTAPAKLAPGAEVELVADVDLAALTPADHVLPVQIITSGGAILWSLAIEGQPEAGN
                     FRGAVSFGADGFSLASSGLSVDLDFRDDGTIAGRVDTDVSLLWPQPLALTGTWTAAGD
                     VTIEMRDRLPAEGWRHSPVARDLGRVLLMTGTRTANGLEGTLTHTITGMRAAPVVTQG
                     AFMLRREGPLTGIVYAPDFTPKDATAPTWLAPPDLDTEACDGLGDLYATDLTLPEPST
                     ACDACTAGTCSPDDMVACGVDLRAASYNLESVLAALHGGGEVKPPTGQWTWDDCAAEE
                     PAYTVGLACLDIAALRCADALVRGGSIQTPGAWGEALRGLAAIFAADEGLAADLLATE
                     AQIDAAFAFKDKIGEPSPDAFARELAILVADRERIAAALAPTLTPAYPAGLVYIEDHG
                     GSYPTTNAHLAPLQLAAGFARTTAQWARLAHRAGQHPEDVRAAVRLAAIAMHAAGAEL
                     HARLADNPAAVPGLHALGTALELLGEVHEELAPGATPFGYPAAYVPLALSPEDIAHNR
                     SNFDAVLALAADEVTQFGQVAADAWQRARDYEQKTHALEVTASQMEAEYDGKLRALCG
                     SLPGDTAPALANCGEDGGQIADLRAGVQAGGLRIRHAGQASENNLHSIVVEEDRFGRV
                     VALALSLSEEIDDAHGRIFQVKDKYGEHRSTVQQAEAMAECGRVRSNSSAEKEALEAG
                     CSSEMIAQMTAGPVILGVSTPNPMGLIFASKECEAKKESLDKHTENQCLSVLGQAGLQ
                     NSLDDLQRNEDEAITAINAEIDQMIRNSDLEVQRAASVALIRNIRAEGLLLQIETEEA
                     GLAHSTAMTAMLSAYMEAAALMQEKARAMGLMVEDSPDNALTHPHFLQARLEAAGRVL
                     PARERTIRRVYLALRALEYELNQGLPSLRESLAAARSPEDITGLMTCLAGIAEDYRLE
                     HGYGQPYVTEVSLRADIFGMTSDVPDVDGSPATPAEQFAALLQDPLHGQADGGVALPF
                     ALSAFENAQFSTLLCDDRIDKIEVKLVGDFLGDKEAEVMLTRQGLAGVRRCDGADLPQ
                     WSAYVPYGFEREQIVIQAGVGDWGTAGPNAGYAAWPVHGEQWTLTIPPPEQAPANLDL
                     DLEHVSDVVLRLHHRAGTIAPAGQGTFTPTCG"
     gene            complement(4501..4992)
                     /locus_tag="VGB85_34135"
     CDS             complement(4501..4992)
                     /locus_tag="VGB85_34135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139194.1"
                     /translation="MNAFQGAVAPEFELTVEESAEARPLPGFHVTRTRTFFVDNYGQV
                     YLLAAPGHRAMLQRQDELPADAEPTRDIADPDLWMMARAAASLGRGEPELSPLTLADY
                     YAWRTRLFRVQGGRVFELKRDGGVRFERVAALPAGAVPFTEAEAEQLGQDVHDAAGCI
                     DAA"
     gene            5118..5474
                     /locus_tag="VGB85_34140"
     CDS             5118..5474
                     /locus_tag="VGB85_34140"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY0139195.1"
                     /translation="MPRELREPKDLGKDFVTTEEVSEVAGVGRPSLYEWLREGLLPAP
                     KTTGGRGIIAKWPPSTLKIAKFVRKQRELGFGLSEIRPRIIAAFGQAVLEVLSEPRGP
                     RARARKGKKLAKMRKN"
     gene            5541..5807
                     /locus_tag="VGB85_34145"
     CDS             5541..5807
                     /locus_tag="VGB85_34145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139196.1"
                     /translation="MPARARDHVTTEELLAATSSTRETLYRWVAQKLLPRPRIATDAG
                     GRQFAAWAPDVLQRARLIAASEGKGLTVAEIAALVEAHCRRTAG"
     gene            5804..6166
                     /locus_tag="VGB85_34150"
     CDS             5804..6166
                     /locus_tag="VGB85_34150"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY0139197.1"
                     /translation="MSVATRSSAPRDYATSDEVSAVAGVDRVALREWVRAGLLPKSEW
                     TGGRGVIAKWPRSTLKIAAFVRSQRELGFGLQDIRARIVAAFGPKVLDLLAEPCSPRV
                     QNKTKKTAIKGTKRRAKP"
     gene            complement(6527..6934)
                     /locus_tag="VGB85_34155"
     CDS             complement(6527..6934)
                     /locus_tag="VGB85_34155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139198.1"
                     /translation="MEKTGESYSIARKHLLDNQEVASPNQVRSWRLSIDADDLEHIGL
                     AFLAEGEAEFAASLSIAEASALFMRLSKLKEEGQNSLVVEHSGQRYNLEMPEGELEIL
                     WAHLGQRLGSNAMLIAQRHGPDRVRALLGTEGP"
     gene            complement(7287..7418)
                     /locus_tag="VGB85_34160"
     CDS             complement(7287..7418)
                     /locus_tag="VGB85_34160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139199.1"
                     /translation="MPPLRTRDDLRARLVWQIAGEVTQGRLIEAIGRRLRWFGIHGA"
     gene            complement(7705..>8216)
                     /locus_tag="VGB85_34165"
     CDS             complement(7705..>8216)
                     /locus_tag="VGB85_34165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139200.1"
                     /translation="AHFADRAPHSRGPSAGLGFFDDVAEMAGAPEMLGTGRWEIANVT
                     GDGAGDLVRVGHREIGLWVHWRARLAELEADEIVILSDVDGSGTFDLLRVEPTARSPF
                     ALCHPVARDLGSRRTCSSAPRRATSAWAVSGGERISDRQRPVVKPDKKVRNVINWRNS
                     KSLHDLCAR"
BASE COUNT         1250 a         2777 c         2871 g         1318 t
ORIGIN      
        1 aacgctcgat cacaggcagg tcgagcgacc aaccgaggcc gagcacgccg gcaccaccgc
       61 ccgcgtggta gcggagcgcg agctggggcg catggccggc gcggccgggc gggacctcca
      121 gcgggtagtt cagcgacggc aggccgctcg cggggctgac ctcgtatgcg cgcccgaagc
      181 cctcgatcga cgcggggccc ttggggatgt tgatggcggt ggccagcgtg gcgctggtgt
      241 ccgcgagtgc cagcgcgggc gccaggaggg cgcaaagcgc cagcatccag cgggcacgga
      301 gcagagaatc gggcggcatg acgggtcctt tcggcgagtt tctgagcgcc agcagatgtg
      361 tggcggatat ctggaaagcc acgccgagcg gcgcagacgc gttttctgat cgctagctga
      421 tatctggcgg atacctgaga cctcgcgccc gtcttcgggc gccgggggtc acccgcaggt
      481 cggggtgaag gtgccctgac cggcgggggc gatggtgccg gcgcggtgat gcaggcgcag
      541 cacgacgtcg gagacgtgtt cgaggtcgag gtcgaggttc gcgggcgcct gctcgggcgg
      601 tgggatcgtc aaggtccact gctcaccgtg aacgggccag gccgcgtagc ccgcgttcgg
      661 gccagcagta ccccagtcgc cgacgccggc ctggatcacg atctgctcgc gctcgaagcc
      721 gtagggcacg taggccgacc attgcgggag gtcggcgcca tcacagcgac gcacgcccgc
      781 gaggccctgg cgggtgagca tgacctcggc ctccttgtcg ccgaggaagt caccgacgag
      841 cttgacctcg atcttgtcga tccggtcgtc gcacagcagc gtcgagaact gggcgttttc
      901 aaaggccgag agcgcgaagg gcaacgcgac gccgccgtcc gcttggccgt gcagggggtc
      961 ttgcaggagc gcggcgaatt gctcggccgg ggtcgcgggg ctaccgtcga cgtccgggac
     1021 gtcggacgtc atgccgaaga tgtcggcgcg gaggctgacc tcggtgacgt agggctggcc
     1081 gtagccgtgc tcgagacgat aatcctccgc gatgccggcg aggcaggtca tgaggccggt
     1141 gatatcctcg ggcgagcgcg ccgccgcgag gctctcacgc agggacggca gcccctgatt
     1201 cagctcgtat tcaagggcgc gcagcgcgag gtacacccgg cggatcgtcc gctcgcgcgc
     1261 cggcagtacg cggccggcgg cctcgaggcg ggcttgcagg aaatgcgggt gcgtcagggc
     1321 gttgtcgggg ctgtcctcga ccatcagtcc catcgcgcgc gccttctcct gcatgagcgc
     1381 ggcggcttcc atgtaggcgc tcaacatcgc cgtcatggcg gtgctgtggg ccagcccggc
     1441 ctcctcggtc tcgatctgaa gcaggagacc ctcggcccga atgttgcgga tgagggcgac
     1501 cgacgcggcg cgctggacct caaggtcact gttgcggatc atttggtcga tctcggcgtt
     1561 gatcgccgtg atcgcctcgt cctcgttgcg ctggagatcg tcgagcgagt tttgcaggcc
     1621 cgcctgcccg agcacactca ggcactggtt ttcggtgtgc ttgtcgaggc tctccttctt
     1681 ggcctcgcat tctttgctgg cgaagatgag gcccatcggg ttcggcgtgg agaccccgag
     1741 gatcaccggg cccgcggtca tctgggcgat catctcgctg ctgcaacccg cttcgagggc
     1801 ctccttttcg gcggagctgt tgctgcggac gcgcccgcac tcggccatgg cctcggcttg
     1861 ctggacggtc gagcggtgct cgccgtactt atccttgacc tggaagatgc gaccgtgcgc
     1921 gtcgtcgatc tcttcgctaa ggctgagcgc gagcgcgacc actcggccga aacgatcctc
     1981 ttccaccacg atcgagtgca ggttgttctc gctggcctgg ccagcgtggc ggatgcggag
     2041 accccccgcc tggacccccg cgcgcaggtc ggcgatctgg ccgccgtctt caccgcagtt
     2101 cgccagcgcc ggcgcggtgt cgccgggcag gctgccgcac agggcgcgca gcttgccgtc
     2161 gtactcggcc tccatctgcg acgcggtgac ttccagggcg tgggtcttct gctcgtagtc
     2221 gcgggcccgt tgccaggcgt ccgcggcgac ctgtccgaat tgcgtgacct cgtcggccgc
     2281 gagcgccagc acggcgtcaa agttgctgcg gttatgggcg atgtcctcgg ggctcagcgc
     2341 cagcgggacg tacgccgccg ggtagccgaa gggtgtcgca ccgggggcga gttcttcatg
     2401 gacctcgccg agcaactcca gggccgtgcc gagcgcgtgc aggccgggca cggcggcggg
     2461 gttgtcggcg aggcgtgcgt gtagctccgc gcccgctgca tgcatggcga tcgccgcgag
     2521 gcgaaccgcg gcgcgcacat cctcggggtg ctgaccggca cggtgggcga gtcgggccca
     2581 ctgggccgtg gtccgcgcga agccggcggc gagctggagc ggcgcgaggt gggcgttggt
     2641 cgtggggtag ctccctccgt gatcctcgat gtacacgagg ccggcgggat aggccggggt
     2701 cagcgtcggc gccagggcgg cggcgatgcg ctcgcggtcg gcgacgagga tcgccagctc
     2761 gcgcgcgaag gcgtccgggc tcggctcgcc gatcttgtcc ttgaacgcga acgcggcatc
     2821 gatctgggcc tcggtcgcca ggagatcggc ggcgagccct tcgtccgcgg cgaagatggc
     2881 cgcgagaccc ctgagcgcct cgccccacgc gccgggcgtc tgaatgctcc caccgcgcac
     2941 cagcgcgtcg gcgcagcgaa gggcggcgat gtcgagacac gccaggccaa cggtgtacgc
     3001 gggctcttcg gcggcgcaat catcccacgt ccactgtccg gtcggaggct tgacctcgcc
     3061 accgccatgc agcgcggcga gcacggactc gaggttgtac gaggcggccc ggagatcgac
     3121 gccgcaagcg accatgtcgt ccggtgagca agttcccgcg gtgcaggcat cgcaggcggt
     3181 ggacggttcc ggcagcgtca gatccgtggc gtagaggtcg ccgaggccgt cacatgcctc
     3241 ggtatcaaga tcgggaggcg cgagccaggt cggcgccgtc gcatccttcg gggtgaagtc
     3301 cggcgcgtag acgatgccgg tgagcgggcc ctcgcggcgc aacatgaagg cgccctgcgt
     3361 caccacgggt gcggcgcgca tgccggtgat cgtgtgggtg agcgtgccct cgagaccatt
     3421 ggcagtgcgc gtgccggtca tgagcagcac gcgccccagg tcgcgcgcga ccgggctgtg
     3481 gcgccagccc tcggcgggga ggcggtcgcg catctcgatc gtgacgtcgc cggccgccgt
     3541 ccaggtgccg gtcagcgcga ggggttgcgg ccacagcagg ctcacgtcgg tgtcgacgcg
     3601 accggcgatc gtgccgtcgt cgcggaagtc gagatcgacg gacaagccgc tgctcgccag
     3661 gctaaagccg tccgcgccga agctcacagc gccgcggaag ttgccggcct cgggctgccc
     3721 ctcgatcgcg agcgaccaca agatggcgcc gccgctggtg atgatctgaa ccggcaggac
     3781 atgatcggcg ggcgtgagcg cggcgaggtc gacatccgcg accagctcga cctccgcgcc
     3841 aggggcgagc ttcgcgggcg cggtgtccag cgtcagcgcc gggttggctg ccgcgagccg
     3901 gtaggtgagc ccctcggtgc catcgttgcg gaggacggtg tgtgcccggg tctcgcccac
     3961 cgcgagcacg gtctgccgct cggtgagcgt cggcgtgccg gcgaagtgca gcgccgtcgc
     4021 gtcccctgcc tcgctgtggc agcggccgtg caggtcgcag acctggcccg gctcgcaccc
     4081 ggccgcatct tcgcagccgg cgacgcagat ccgcagctcg cacaggtcgc ccgcgtcaca
     4141 gtcggcgtcg acctggcagg ggctgccgtc gggccagggg ttcgcggggg gtggaggctc
     4201 gggctcgggc cccgagtcgt cggccgtcgc ggctccggtg gcgggggtag tgcctgtaga
     4261 ggtggagccg tcttgcgcga cgtcatcggg cgcgcaggac agcgagaaca acagggccga
     4321 cgcggcggcc tggaacatcg gtgttcgttt cacggcacac ctctcctgtc gcgctcacgc
     4381 gagcgctgtt ttcgggcgat ggtgtctcgg cggcaaaatg cagccgcggc gccccacagg
     4441 tgagcccgcc acgagactca cgcggcgcgt gtctgtcagc tccggcgccg ggccggtcgt
     4501 tcaggcggcg tcgatacagc ccgccgcgtc gtgaacgtcc tggccgagct gctccgcctc
     4561 tgcttcggta aatggcaccg cacccgccgg tagcgcggcg acacgctcga agcgcacgcc
     4621 gccgtcccgc ttcagctcga agactcggcc cccctgcacg cgaaataggc gtgtgcgcca
     4681 ggcgtagtag tccgcgagcg tcagcgggga cagctcaggc tcgccgcgcc ccagcgacgc
     4741 cgccgccctg gccatcatcc acaggtcggg gtcggcgatg tcccgggtcg gctccgcgtc
     4801 ggcgggtaac tcgtcttgcc gctggagcat cgcccggtga ccgggcgctg cgagcaggta
     4861 gacctgcccg tagttgtcga cgaagaatgt ccgagtgcgg gtgacgtgga agcccgggag
     4921 tggccgggct tccgcgctct cctcgaccgt cagctcaaat tctggcgcca ccgcgccctg
     4981 gaacgcattc atacgggtct cctccaaaac tgttgaccaa acataaccca attaatgatt
     5041 tttcaaatta aataattgtc ggcatccggt cgccagtctg gcgctcctgg cggccggtgg
     5101 tgtacgctgc ccccgacatg ccgcgagaac tccgggaacc caaggacctg ggcaaggact
     5161 tcgtgaccac cgaagaggtc tccgaggtcg ccggagtcgg gcgcccgtcg ctctacgagt
     5221 ggctccgtga gggactactt ccggcgccga agaccacggg aggacgcggc atcatcgcca
     5281 agtggccgcc ttcgacgttg aagatcgcca agttcgtccg caagcagcgc gagctgggct
     5341 tcggactgag cgagatccgg cctcgcatca tcgcggcgtt cggccaggcg gtgctcgagg
     5401 tcctgtccga acccaggggc ccacgcgctc gcgcccgcaa aggcaagaag ctcgccaaaa
     5461 tgcggaaaaa ctgatgatcg gtgaatcggg cgcgtcctca ccatctggcg ccgccgcgcc
     5521 cgatcttcta ccgtccatgg gtgcctgcgc gcgcacgaga ccacgtcacc accgaggagt
     5581 tgctggccgc caccagctcc acccgcgaga cactgtatcg ttgggtcgca cagaagctcc
     5641 tgccccggcc gcggatcgcc accgacgcgg gcggccgcca gttcgccgcc tgggcgcctg
     5701 acgtcctcca gcgcgcgcgc ctcatcgccg ccagcgaggg caagggcttg accgtcgcgg
     5761 agattgccgc cctcgtggaa gcccattgcc gacggacggc aggatgagcg tggccaccag
     5821 gagcagcgcc cccagggact acgcgaccag cgacgaggta tcggccgtag caggagtcga
     5881 ccgtgtagcg ctgcgcgaat gggtgcgcgc ggggctcctg ccgaagtcgg agtggacggg
     5941 cgggcgaggg gtcatcgcca agtggccgcg ctcgaccctg aagatcgccg cgttcgtccg
     6001 cagccagcgc gagctgggtt tcggcctgca agacatccga gcccggatcg tcgcggcgtt
     6061 tggcccgaag gtgctggact tgctcgccga accctgcagc ccccgggtac agaacaagac
     6121 gaaaaagacg gcgataaaag gcaccaagcg gcgcgccaag ccgtaggaag gccgcgaatc
     6181 tctcgcttcg ccctcggagt cgacgaacgg tcgatcctcg gtctacgctc cgaactattg
     6241 cctgcgatgg ctgatcggca agtcaacttc atccgcgccg tggtcgcccg tcagtgacac
     6301 ggtggtgacc cctgattttt gggccgcgat cacagccgac gttgcggccg tgctagccga
     6361 cgacccccat ttctgggaga ggctccgaga gcctcaccga gtcgaacgaa ctcggcgatg
     6421 ttgtagaggc cgaacccaga gccacgctgg gcattaacgc accgcacgct ccggtcgatc
     6481 gggagacgcg gcacccgacc cggtgtcgcg tctttggagc cgacgcttac gggccttccg
     6541 tgccgagcag cgcgcgcaca cggtcgggcc catgcctctg ggcgatgagc atggcgttcg
     6601 acccgaggcg ttgacccaag tgtgcccaca agatctcgag ctccccctcg ggcatctcga
     6661 ggttgtagcg ctggccgcta tgctcaacca ccaggctgtt ctggccctcc tccttgagct
     6721 tcgagagcct catgaaaagg gccgatgcct cggcgatcga gaggcttgcc gcgaattcag
     6781 cttcgccctc cgcgaggaag gcgaggccga tgtgctcaag gtcgtcggcg tcgatgctca
     6841 ggcgccagct ccggacctgg ttcgggcttg ccacttcctg gttgtccagg aggtgtttcc
     6901 gcgcaatcga gtaagactcc ccggtcttct ccacgacccc atccagcagg cgtatgcggc
     6961 cggcgggact ggcatcgcca gcgatgctgc tcggatcttg agctcgcgga cgatcagcgt
     7021 cggcacgccg tgtttgccgc cgtcccctac acgcgacagc agccgctttt ggtccgcgca
     7081 ggaccgactg tttgctcgga cctcctcgcg caccaaataa tggtccgaaa attaagtccg
     7141 cacggcacat cagcggccgc agggtcgagc cgacctcgac cgagttcttt gatgttctga
     7201 cagaaaatcc agaagagcct aaccctgggt gcgacccacg cgacgggcgt acggcggagc
     7261 agcccgatct ccgcgcccta cggctcctac gcgccatgga tgccaaacca cctcaagcgc
     7321 cggccgatcg cctcgatcag ccgcccctgc gtgacctctc ccgcgatctg ccagaccagc
     7381 cgtgcgcgca ggtcgtcacg cgtgcgcagc ggtggcatcg gcctggcacg gtggatgctg
     7441 cgccgccagg ctgcttggca cgatcggtcg agaatcgcga ggtcaccctg cggggacagc
     7501 tccgggggtg gacagtcacg acgcatgctc gccccgtgcg ctcggcttcg gtgtcacagt
     7561 gccgcgcgtc cgcggcgccc gagacagccg gccgctcgat caccgcgtca cccgcgtgcg
     7621 ggcggcaccc gcgaggttcg caacgttttg atctctggaa actcctcgat ctccatcatc
     7681 tcgaccgctt ctactccgtc gatcttaccg cgcgcagagg tcgtgtagac tctttgagtt
     7741 tcgccaattt ataacgttgc gcactttctt atccggcttc accacgggcc gttggcgatc
     7801 agaaatcctt tcgccgccgc tcaccgccca cgccgaggtc gcgcgccgtg gagccgacga
     7861 acatgtccgc cgggatccca agtcgcgcgc caccggatgg cacagggcga acgggctgcg
     7921 agccgtcggt tcgacgcgca gtagatcgaa ggtgccgctc ccgtcgacgt cgctcaaaat
     7981 cacgatttcg tcggcctcca gctcggctag tcgcgcgcgc caatggaccc acaggccgat
     8041 ttcgcggtgg ccgacccgca cgaggtcgcc cgcgccgtcc ccagtcacgt tggcgatttc
     8101 ccagcggccg gttcccaaca tttcgggggc gccggccatt tcagcaacgt cgtcgaaaaa
     8161 ccccaggccg gcgctcgggc cacgagaatg cggcgctcgg tcggcaaaat gcgcag
//
LOCUS       DASWNJ010002051         2692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_423210, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002051 DASWNJ010000000
VERSION     DASWNJ010002051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423210"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            455..>2692
                     /locus_tag="VGB85_34170"
     CDS             455..>2692
                     /locus_tag="VGB85_34170"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEY0139201.1"
                     /translation="MRQTAVGGGGARAASEASLIFARGGAAEASGTGSSDTGSAVPTT
                     TAAGTEGAARRDPAPSPERLADIVLAGLVLTLTTLPFDLSLGALIFAGPLAAHVGVGV
                     GLVLFSALVLGLVGERLSGRRGTVVLIQDKTAVILAAAAAVVTGEVLARDPNADPLPT
                     VLVLLALGTLITGATMWTLGRLRLGRLVRFIPYPVVAGFLGGTGAVLLLGAVEVMAHR
                     PFAAGHLAPALLVRWLPGLLFGAFLAWWSGRSRSKLAIPQALAVGTALCFAALWAGGV
                     APTAAMADGWLLGPLPGGVLWPPALPTAATIDGTALLHGLPNLGPLAIVAVLGLLLNI
                     SSAEAVTDSEYDLDRELRANGVANLAAGCSAGLPGYMAVGSTLLLHRMAVRSRWPAIF
                     AALANGAFLLIGAAPLAWIPRWLLGGLLVFLGIDLLGHALRDARAQLPVREFMLVLAI
                     LTVIVAWGFLAGVGVGLLISVALFAVDSSRVDLIRQSASGAELRSNIARTSRHQRVLD
                     AHGAQIRVLQLQGYIFFGSAHRLLHDVSGELDGGAALRFVVADFRQVVGLDCSAAATL
                     ARLQRRLQVRGVALVCTGLTPAVERPLTIAGCELHGASAQVHADLDHGLEFCEERLLA
                     DAGVSREEVRLADEFAALAGDASLLPRLLAFAAHVDLPAGAPVFAAGDPGDGLYLIES
                     GSLTAWLELGDGRRRLRTMGPGTMIGEAGPYLQAQRSAAVVCDTPARLHHLSNAALAR
                     MHAEAP"
BASE COUNT          294 a         1032 c          963 g          403 t
ORIGIN      
        1 tttgcccgag cgtattgccg atcagctcga agtcgccgcg ctgggtgacc tgcacccgca
       61 gcgccggcgc agccgaggcc gaggccacca tcgacaacca acccgcgatc gcgggcagac
      121 ccactccaat actctgtttt cgcatgacga tgactccttg aaggacacgt cgaccgtacc
      181 ggcaacgcgg gccggcggaa tttcaggcga atttcaatgg ccgcgtgcgc gcgagaatgc
      241 agcgccgtaa agccagcgcg gcatcttcgc gctgtggaat ggttccacct cggactactc
      301 aaacgcggtc gccacgccga cgctcgaggc ggtgcccggc gacgtaggtg catcggcccg
      361 gatgaccggc gtcggcggca gacgcatcgc ttgccgagcc ccctgaaagc gcggtgaaat
      421 tttcccgcgg gcgcgcgttg atagcgtcgg gaccatgcga cagacagcgg tgggcggtgg
      481 gggtgcgcgg gcggccagcg aagcgagcct gatcttcgcc cggggcggcg ccgcggaagc
      541 atccgggacc ggctcgtcgg acaccggcag cgccgtgccc acgaccaccg cggccggcac
      601 cgaaggtgcc gcgaggcggg atcctgcacc gtcccccgaa cgcctggcgg acatcgtcct
      661 cgccggactg gtcctcaccc tcaccacgct accgttcgac ctgtcgctcg gggcgctgat
      721 cttcgccggc ccgctcgcgg cgcacgtcgg ggtcggcgtc ggcctggtgc tgttctccgc
      781 gctggtcctc gggctggtcg gcgagcggct gagcgggcgg cgcgggaccg tcgtgctgat
      841 ccaggacaag accgcggtga tcctcgcggc cgccgcggcc gtcgtcaccg gcgaggtcct
      901 cgcgcgcgac ccgaacgccg acccgctgcc gacggtgctc gtgctgctcg cgctcggcac
      961 cctgattacc ggcgcgacga tgtggactct cgggcgcctg cgcctcgggc ggctggtccg
     1021 cttcatcccg tacccggtcg tcgccggctt cctcggcggc accggggcgg tgctgctgct
     1081 cggcgcggtc gaggtgatgg cgcaccggcc gttcgccgcc ggccatctcg cgccggcgct
     1141 gctggtccgc tggctgccgg ggctgctgtt cggggcgttc ctcgcctggt ggtccggtcg
     1201 cagccgcagc aagctggcga tcccgcaggc gctcgcggtc gggacggcgc tgtgcttcgc
     1261 ggcgctgtgg gccggcggcg tcgcgccgac cgcggcgatg gccgatggct ggctgctcgg
     1321 gccgctgccc ggcggcgtgc tgtggccgcc ggcgctgccg accgcggcga cgatcgacgg
     1381 gacggcgctg ctccacggcc tgccgaacct cgggccgctg gcgatcgtcg cggtcctcgg
     1441 gctcctgctc aacatctcga gcgcggaggc ggtgaccgac agcgagtacg acctcgaccg
     1501 cgagctgcgg gccaacggcg tcgccaacct cgcggctggc tgctcggcgg ggctgccggg
     1561 ctacatggcg gtcggctcga cgctgctcct ccaccggatg gcggtgcgca gccgctggcc
     1621 ggcgatcttc gcggccctgg cgaacggggc cttcctgctg atcggcgccg cgccgctggc
     1681 gtggatcccc cgctggctgc tcggcgggct gctggtgttc ctcggcatcg acctcctcgg
     1741 ccacgcgctg cgcgacgcgc gggcccagct gccggtccgc gagttcatgc tcgtgctcgc
     1801 gatcctcacc gtcatcgtcg cgtggggctt cctcgccggc gtcggcgtcg ggctgctgat
     1861 ctccgtcgcc ctgttcgcgg tcgacagcag ccgcgtcgac ctgatccgcc agtcggcctc
     1921 cggcgccgag ctgcgcagca acatcgcccg cacgagccgc caccagcgcg tcctcgacgc
     1981 ccacggcgcg cagatccgcg tgcttcagct gcagggctac atcttcttcg gctcggccca
     2041 ccgcctgctc cacgacgtca gcggcgagct cgacggcggc gccgcgctgc gcttcgtcgt
     2101 cgccgacttc cgccaggtcg tcggcctgga ctgctcggcc gcggcgacgc tggctcgcct
     2161 gcagcgccgc ctccaggtcc gcggcgtcgc cctcgtgtgc accggcctga cccccgcggt
     2221 cgagcgcccg ctgacgatcg ccggctgcga gctccacggc gcgtcggcgc aggtccacgc
     2281 cgacctcgac cacggcctcg agttctgcga ggagcggctg ctcgccgacg ccggcgtgtc
     2341 gcgcgaggag gtccgcctcg ccgacgagtt cgccgcgctc gccggcgacg cgtcgctgct
     2401 gccgcgcctg ctggcgttcg ccgcccacgt cgacctgccc gccggcgccc cggtgttcgc
     2461 ggcaggcgac ccgggcgacg gcctgtatct catcgagtcc ggctcgctga cggcctggct
     2521 cgagctcggc gacggtcgcc gccgcctgcg cacgatgggc cccgggacga tgatcggcga
     2581 ggccggcccg tacctgcagg cgcagcgctc ggccgcggtc gtctgcgata cgccggcccg
     2641 cctgcaccac ctctcgaacg cggccctcgc ccgcatgcac gccgaggcgc cc
//
LOCUS       DASWNJ010002052         1665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_423451, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002052 DASWNJ010000000
VERSION     DASWNJ010002052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423451"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VGB85_34175"
     CDS             <1..454
                     /locus_tag="VGB85_34175"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEY0139202.1"
                     /translation="VFGLNQHIVSGFGRSRLTLVNMLVAIALGVALLTLCIPRAGVDG
                     AAWGIGLTYVGLNVLWAIEARVIAGGWHYERSIGVVLVLTACAAAAMAAGWLGGAAVF
                     GTSVTGDVGARLLALAAFAAVFAPGMLALRRRGRFAGDAAPKDSSAGA"
     gene            complement(440..1063)
                     /locus_tag="VGB85_34180"
     CDS             complement(440..1063)
                     /locus_tag="VGB85_34180"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0139203.1"
                     /translation="MTVQVRPEHYRFERYDDLERWGSYWYQIRAALRTEPRRVLEIGS
                     GTRVFRSYLENRGIEVRSVDIDASREPDFVADVTDLDHTLPAGVRFDCVAAFQVLEHL
                     PHARFEDCLAGLARRAAPWCLLSLPHHGVALRMSVNVAGLRVSRGVSLPFPWQKRFDG
                     EHHWELGWGHSVRSVTRTIGKYFEVVERGFVPENPYHYLWVLRSRAG"
     gene            complement(1060..>1665)
                     /locus_tag="VGB85_34185"
     CDS             complement(1060..>1665)
                     /locus_tag="VGB85_34185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEY0139204.1"
                     /translation="EVALMCDKLGIDVWEVIDAAATKPYGFMKFTPGPGVGGHCIPLD
                     PHYLSWKLRTLNYNARFVQLADEINSGMPGYWVGKVQDALNDHEKAMRGSTVLVLGVA
                     YKKDIDDVRESPALDIIALLQQKGARVIYHDPHVPRIAHDTTELLAETDLRAALDAAD
                     CVMIVTDHSSYDWRDIAGRARLVVDTRRALPRTLADREAAQ"
BASE COUNT          220 a          540 c          639 g          266 t
ORIGIN      
        1 cgtgttcggg ctcaatcagc acatcgtcag cggctttggc cgcagccgcc tgacgctggt
       61 caacatgctc gtggcgatcg ccctcggcgt cgcgctgctg acgctttgta tcccgcgcgc
      121 gggcgtcgac ggggcggcgt ggggcatcgg cctgacctac gtcgggctca atgtgctgtg
      181 ggcgatcgag gcccgcgtga tcgccggtgg ctggcactac gagcgcagca tcggggtcgt
      241 gctcgtgttg accgcgtgcg cggccgcggc gatggccgcg ggctggctcg gcggcgcggc
      301 cgtgttcggg accagcgtca ccggcgacgt cggggcccgg ctcctcgcgc tggcggcctt
      361 cgccgcggtg ttcgcccccg gcatgctcgc gctgcgccgt cgcgggcgat tcgccggaga
      421 tgctgccccg aaggacagct cagccggcgc gtgagcgcaa gacccagaga taatggtagg
      481 ggttctcggg cacgaagccg cgctcgacga cctcgaagta cttgccgatc gtccgcgtca
      541 cgctgcgcac ggagtggccc cagccgagct cccagtggtg ctcgccgtcg aagcgctttt
      601 gccaggggaa gggcagcgag acgccgcggc tgacgcggag cccggcgacg ttgaccgaca
      661 tgcggagcgc gacgccgtgg tgcggcaggc tcagcaggca ccacggggcg gcgcggcgcg
      721 ccaggccggc gaggcagtcc tcgaagcgcg cgtgcggcag gtgctcgagg acctggaacg
      781 cggcgacgca gtcgaagcgc acgccggccg gcagggtgtg gtcgaggtcg gtgacgtcgg
      841 cgacgaagtc gggctcgcga ctggcgtcga tgtcgaccga tcgcacctcg atcccgcggt
      901 tctcgaggta gctgcgaaac acccgcgtgc cgctgccgat ctcgagcacg cggcggggct
      961 ccgtgcgcag cgcggcgcgg atctgatacc agtacgagcc ccagcgctcg aggtcgtcgt
     1021 agcgctcgaa gcggtagtgc tcgggccgga cctggaccgt cactgggcgg cctcgcggtc
     1081 cgccagggtg cgtggcaggg cgcggcgggt gtcgacgacg aggcgggcac ggccggcgat
     1141 gtcccgccag tcgtagctgc tgtggtcggt cacgatcatc acgcagtccg cggcgtcgag
     1201 ggcggcgcgc aggtcggtct cagcgagcag ctcggtcgta tcgtgggcga tcctcggcac
     1261 gtgcgggtcg tggtagatga cgcgcgcgcc tttttgttgc agcagcgcga tgatgtcgag
     1321 cgccggcgac tcgcggacgt cgtcgatgtc ctttttgtag gcgacgccga gcacgagcac
     1381 ggtgctgccg cgcatcgcct tctcgtggtc gttgagcgcg tcctggacct tgccgaccca
     1441 gtagccgggc atgccgctgt tgatctcgtc ggcgagctgg acgaagcgcg cgttgtagtt
     1501 gagcgtgcgc agcttccacg acagatagtg cgggtcgagc gggatgcagt ggccgccgac
     1561 gccgggcccg ggggtgaact tcatgaagcc gtagggcttg gtcgcggcgg cgtcgatcac
     1621 ctcccagacg tcgatgccga gcttgtcgca catcagggcg acctc
//
LOCUS       DASWNJ010002053         1614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_351703, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002053 DASWNJ010000000
VERSION     DASWNJ010002053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351703"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="VGB85_34190"
     CDS             complement(<1..1242)
                     /locus_tag="VGB85_34190"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0139205.1"
                     /translation="MRADAILLAQVPTTSPIQHDPTEAIGLTPIDQVRRQVATALFGE
                     LVPLPQVGRFQVAKILGTGGMGVVYEAHDPQLGRKVALKLLQPMTTGERARERLLREA
                     QAMAQLQHPNVVGVYEAGVHGDQVYVAMEYVENGTLGEWLRARPRTWQEIVDMLLQAG
                     EGLSAAHAGGLVHRDFKPANILIGSGRARISDFGLARTTATAEDLERTAEDSSGMLLG
                     APLTRTGSLLGTPAYMAPEQLRGEPATPASDQFAFGVVLYESLYGHRPFQGESLGVLM
                     TAIEEGRIARPERKLVVPAKLTAVIRRALARRPEQRFPNMASLLEALGAARRVRWVAP
                     VLGAGIPAAVVVAGMAGLFMYSSPPPASQAAAAAVPAVRCDGGDALAGMWDAQRRAVV
                     EQALIEADINSGTLASLDDYAT"
     gene            1360..>1614
                     /locus_tag="VGB85_34195"
     CDS             1360..>1614
                     /locus_tag="VGB85_34195"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEY0139206.1"
                     /translation="MDSDEALLASWRAGDPRAGRTLFSRHVDGVSRFFFSKVATGVDD
                     LVQRSFLALLERSAQIPAGVPVRAYLFGIARNLLLRRFRDE"
BASE COUNT          217 a          650 c          511 g          236 t
ORIGIN      
        1 cgtcgcgtag tcgtccagcg acgcgagcgt cccggaattg atgtctgcct cgatcagcgc
       61 ctgctcgacc acggcccggc gctgcgcgtc ccacatgcct gcgagcgcgt cccccccgtc
      121 gcaacggacg gccggcaccg ccgccgcggc cgcctgcgac gccggaggcg ggctcgagta
      181 catgaacagc ccggccatgc cagcgacgac caccgccgcc gggatcccgg ccccgagcac
      241 cggcgcgacc cagcggaccc gccgtgcggc gccgagcgcc tcgagcaacg aggccatgtt
      301 cgggaagcgc tgctccggcc tccgcgcgag cgcgcgacgg atcaccgccg tcagcttcgc
      361 cggcaccacc agcttgcgct ccggccgggc gatcctcccc tcctcgatcg ccgtcatcag
      421 cacgccgagc gactcgccct ggaacggccg gtgaccatac agcgactcgt agagcaccac
      481 gccgaacgcg aactggtcgc tcgccggcgt cgccggctcg ccgcgcagct gctccggggc
      541 catgtatgcg ggcgtgccca gcagcgagcc cgtgcgcgtc agcggggcgc cgagcagcat
      601 gccgctgctg tcctctgccg tgcgctcgag gtcctccgcc gtcgccgtcg tccgcgcgag
      661 cccgaagtcg gagatgcgcg cccgaccgct gccgatcagg atgttggccg gcttgaagtc
      721 gcgatgcacc agcccccccg cgtgcgcggc cgacagcccc tcgcccgcct gcagcagcat
      781 gtcgacgatc tcctgccacg tccgcggccg cgcgcgcagc cactcgccga gcgtgccgtt
      841 ctcgacgtac tccatcgcca cgtacacctg gtcgccgtgc acgcccgcct cgtagacccc
      901 gaccacgttg gggtgttgca gctgcgccat cgcctgcgct tcacgcaata gccgctcgcg
      961 cgcccgctcg ccggtggtca tcggctgcag cagcttgagc gcgaccttgc ggccgagctg
     1021 ggggtcatgc gcctcgtaga ccacgcccat cccgccggtg ccgaggatct tcgcgacctg
     1081 gaaccggccg acctgcggca gcggcaccag ctcgccgaac agcgccgtcg cgacctgccg
     1141 ccgcacctga tcgatcggtg tcaggccgat ggcctccgtc gggtcgtgtt ggatcgggct
     1201 cgtcgtgggc acctgggcca gaaggatcgc atccgctcgc atgatcgtca cctctgaacc
     1261 ggacgttcca cgacgagccc gatcgatcac gccgccacga cgaatttctc cgaataaaat
     1321 ttcgcgcgtc gccgcccccg tccagctaca gtccgcggca tggactccga cgaggccctg
     1381 ctggcgtcct ggcgcgccgg agacccgcgc gccggccgga cgctgttttc tcgccacgtc
     1441 gacggcgtgt cgcgcttctt cttcagcaag gtcgccaccg gcgtcgacga cctcgtccag
     1501 cgcagcttcc tcgccttgct cgagcgcagc gcgcagatcc ccgccggcgt gccggtccgc
     1561 gcctacctct tcggcatcgc ccgcaacctg ctgctacgtc gcttccgcga cgag
//
LOCUS       DASWNJ010002054         2478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_423556, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002054 DASWNJ010000000
VERSION     DASWNJ010002054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423556"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1814)
                     /locus_tag="VGB85_34200"
     CDS             complement(87..1814)
                     /locus_tag="VGB85_34200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139207.1"
                     /translation="MSRRLLALALVLDACAEHTREYEDLLDKFGSETNDESGTTTGSA
                     GTPTSGTSTTTTGDSTSATDESAGNSDDNGPNPGDDTGQSSGDTTATSTGLPIDPPPT
                     VEELVCNPEKAEEVGPVSCTYLASADAVEAELRDDGEVVATGPAGSSLIFPVISAPHN
                     NPGSTITVVVRDAAGQTAETSIYQPSTVKDPGTAIWTKLEPNDGAFSMASAAALQGDH
                     LIAAGIHFKNPLVVGTLRRYDKAGDWLATDEGWSRTHDTWTKLDWLKTGSFGPTGLAV
                     DAEGNIILVGLGFHDDQPRSYLAHFFPDGTLNWEKPGDVGTEARAVGVQSDGTIWVAG
                     AKRTGVNPEFWDMEVSVFGPDKTPYGPFPYSDPEDKKNERSERGRAVVVLKNGNVVVV
                     GEREILDPNMVFPITRGVALLFEGKGKFLGEWTSSGDKLKYDAILAAVATDEGFATCG
                     YTHTDPDEPGSKKQILIRWHGEDLHEVKAPRLESTMGAAICNALGYNMEGATIVGAQV
                     AKNGQNNDQWIFAVEDAASPRVDYREHNGASNGDDRVQALDCKYKCAWVGAETVDGAL
                     PWVAGLVRP"
     gene            complement(1851..>2478)
                     /locus_tag="VGB85_34205"
     CDS             complement(1851..>2478)
                     /locus_tag="VGB85_34205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139208.1"
                     /translation="AAAISALCWAIETMRTYQAELMTTERDRLTIPAEVVRLESLATG
                     LAAPCLAKKSEPPSAATASTGAGAPPTADPRQVSDQREGPEPPPAPTRIRRTRPQIAI
                     GSTLLITGAGLAAGFAGCFVASGHERAQISALGARAEEAGRDPTNEEWAEAATADARA
                     IRLARTGTTLGVFAVASVLAGFVVLMLPRRSTSRVQARPVGPGVRINF"
BASE COUNT          330 a          842 c          856 g          450 t
ORIGIN      
        1 gcatctgcgt cgacagctac atcccggccc tgacgcagac ggccgagatg atcctcgagc
       61 agtgttcggt cttcgtcccg cagtgactac gggcggacca ggccggcgac ccacgggagg
      121 gcgccgtcga ccgtctcggc cccgacccac gcgcacttgt acttgcagtc gagcgcctgg
      181 acacggtcgt cgccgttgct ggcgccgttg tgctcgcggt agtcgacgcg cggcgaggcg
      241 gcatcctcga cggcgaagat ccactggtcg ttgttctgtc cgttcttggc gacctgcgcg
      301 ccgacgatcg tcgcgccctc catgttgtag ccgagcgcgt tgcagatcgc ggcgcccatg
      361 gtgctctcga gccgcggtgc cttgacctcg tgaagatcct cgccgtgcca gcggatcagg
      421 atctgcttct tgcttccagg ctcgtccggg tccgtatggg tgtagccgca ggtggcgaag
      481 ccctcgtcgg tcgcgaccgc cgcgaggatc gcgtcgtact tcagcttatc cccgctcgac
      541 gtccactcgc cgaggaactt gcccttgccc tcgaacagca gcgccacgcc gcgtgtgatc
      601 gggaagacca tattcgggtc gagaatctcg cgctcaccaa cgacgacgac gttgccgttt
      661 ttcagcacca cgaccgcccg gccgcgttcg ctccgctcgt tcttcttatc ctccgggtcg
      721 ctgtagggga acggcccata gggggtcttg tcggggccaa agacggagac ctccatgtcc
      781 caaaactccg gattgacgcc cgtccgcttc gcgcctgcga cccagattgt gccgtccgac
      841 tgcacgccga ccgcacgggc ctccgtgccc acgtcgcctg gcttctccca gttcagcgtg
      901 ccgtccggaa agaagtgggc gaggtagctc cgcggttgat cgtcgtggaa gcccaggccg
      961 acgaggatga tgttgccctc ggcgtcgacc gcgagcccgg tgggtccgaa gctgcccgtt
     1021 ttcagccaat cgagcttggt ccaggtgtcg tgcgtccggc tccacccctc gtcggtggcg
     1081 agccagtcgc cggccttgtc gtatcggcgg agcgttccga ccacgagagg gtttttaaag
     1141 tggatgcccg cggcgatgag gtggtcgccc tgcagcgcgg cggcggacgc catgctgaac
     1201 gcgccgtcgt tcggctcgag cttggtccag atcgccgtgc ccgggtcctt gaccgtgctc
     1261 ggctggtaga tcgacgtctc ggcggtctgc cccgccgcgt cgcgcacgac gaccgtgatg
     1321 gtgctgcccg ggttgttgtg cggggcgctg atcacgggga agatgagcga cgagccggcg
     1381 ggaccggtgg ccacgacctc gccgtcgtcg cgcagctcgg cctcgaccgc gtcggcgctc
     1441 gccaggtagg tgcaggagac cggcccgacc tcctctgcct tctctggatt gcagacgagt
     1501 tcctcgactg tcggcggtgg gtcgatcggc aagcccgtgc tcgtcgcggt cgtgtcgccc
     1561 gaggactgcc cggtgtcgtc gcccgggttc ggcccgttat cgtcgctgtt gcccgcgctt
     1621 tcgtcggtgg cgctggtgct gtcgcccgtg gtcgtggtcg atgtgccgga cgtcggagtg
     1681 cccgcgctgc ccgtcgtggt gccggactcg tcgtttgttt cgctaccgaa tttgtcgagg
     1741 agatcctcgt actcgcgcgt gtgctcggcg catgcatcga gaacgagggc gagtgcgagg
     1801 aggcgacggc tcataaggca atggtactcc tgctcgggcg gatccgagcg tcagaagttg
     1861 atgcggacgc caggcccgac cggacgcgcc tggacgcgag aggttgagcg acggggaagc
     1921 atcagcacga cgaatccggc gagcacgctg gccaccgcga acacaccgag cgtcgtgccg
     1981 gtccgcgcca ggcggatcgc ccgggcgtcg gctgtcgcgg cctccgccca ctcctcgttc
     2041 gtcgggtcgc gccccgcctc ctcggcccga gcaccgagcg ccgagatctg cgcccgctcg
     2101 tgcccgctgg cgacgaagca acctgcgaac ccagcggcga ggccagcacc ggtgatcagc
     2161 agcgtcgagc cgatggcgat ctgcggccgc gtgcgacgaa tacgtgttgg cgcgggcgga
     2221 ggctcggggc cttcacgctg atcgctcact tgcctcgggt cagcggtcgg cggagccccg
     2281 gcgccggtgc tcgcggtggc ggccgacggg ggctcggatt ttttcgcgag gcagggtgct
     2341 gcaaggcccg tcgccagcga ctcgaggcgg accacctcgg cggggatcgt gagccgatcg
     2401 cgttcggttg tcatcagctc ggcctgatat gtccgcatcg tctcgatcgc ccagcacagc
     2461 gccgagatcg ccgcggcc
//
LOCUS       DASWNJ010002055         3605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_351862, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002055 DASWNJ010000000
VERSION     DASWNJ010002055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351862"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..>3605
                     /locus_tag="VGB85_34210"
     CDS             29..>3605
                     /locus_tag="VGB85_34210"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0139209.1"
                     /translation="MTGSASSNAGPRTIGRFMVLRQIGSGAMGVVYSAYDPELDRRIA
                     IKLLHPSLRYQYDGIGDSRLLHEAQAMARVSHPNVAAIYEVGVVGEQVFIAMELVRGQ
                     TLREWVTARRRSWREVVDVYLQAARGLIAAHEQGIIHRDFKPENALIGDDGRVRVLDF
                     SLARSSTPEPDRRALDEALTTGDVAGIQSAQAVGTPAYMPPEQALGAVADGRADQFSF
                     CVALYEALYGRRPFTGESTTELIKDILRENIRPPASARLPSWLTRALLRGLRPAPDQR
                     WPSMAALVAELERDRGRWLRLASAIALAAVLAGLVLGALELRARWRHAAVEQAAAERL
                     AVLAVNLDQTLAAGRRSEAEAALAAFTGAPEHRDTPALAAAYLDWSRRMRVAADLDAA
                     RLAAARAYAAASDEPGVEAALEQLALLFCAQGAWDAAALLVADLRVHRPALLERPVWV
                     AIAAQAARRSRDLGEFARQAARANAPPELAVLAPVAAALSHGTATGRVAARAFAVQGE
                     PTLALVARIDDDGAELVRKDVALSPVGRVEVPGFAIAGLAPRLRPDGPALLLAHRSEP
                     PEAAVLALASDGARELLRFSSARPLGNATADLDGDGTPSLYLGTGPYARELLALTPDA
                     DGTLRLEHPHPGTDAAHSDLQDMFTADVDGDGRKELVVSAGPWNAFDLRVYRAGAKAP
                     ALELMARRKFGHLVAIGALAGSDGRTRIVAAKTDPYPSRFVFPRDTPNGDPAGFYVLE
                     LRGDHIEVLAHHPWPVAPGQPLSVSMMGVGDVDGDGLVDPVVTLSLGDHSEPLVVVYR
                     QLADGALAPVEIAGINMLMLGQFDDDPAVELLARMMPDDPSVPAPLWVLGAGDTPVPP
                     LALLRPEPASVPAGADPLVARTWARAEQLASLGLLTEAAQTISELASLAQRPEIGDDA
                     RLRAAELYRRADQCAPATALYGQLGERHAVAALQGLAACHEREGRFAEALVDIDALRA
                     RADLDPAVRAAVTRRAEQLRPGSSETPIDLPFDGHLDPGWQIVDPTALRQDLVTGGLA
                     IDAIANRGVLARLPLRCDGEALAVRAELTVERSEWASGFEIAVRSAGGEDLVSLDIAT
                     GGGGGIYSRTIRCGGPDETRTLQILRDTAPDTVAPYVLEVDYRPALGRSVCAVTGSDG
                     VRRSWAFPAPAKLPSGACELVLRARHIVFDGSFWTRLRLDALRVRG"
BASE COUNT          420 a         1363 c         1354 g          468 t
ORIGIN      
        1 cccgagcgcg cccggaggat cgccgtcggt gacggggtcg gcgtcgtcga atgcggggcc
       61 gcggacgatc gggcgcttca tggtgctgcg ccagatcggc agcggcgcga tgggggtcgt
      121 gtattcggcg tacgacccgg agctcgaccg gcggatcgcg atcaagctgc tgcatccgag
      181 cctgcgctac cagtatgacg gcatcggcga ctcgcgcctg ttgcacgagg cgcaggcgat
      241 ggcccgggtc tcgcacccga acgtggcggc gatctacgag gtcggggtgg tcggcgagca
      301 ggtgttcatc gccatggagc tggtgcgcgg gcagacgttg cgcgagtggg tgacggcgcg
      361 cagacgcagc tggcgggagg tcgtcgacgt gtatctgcag gccgcacgcg gcctgatcgc
      421 cgcgcacgag caggggatca ttcaccgcga cttcaagccg gagaacgcgc tcatcggtga
      481 cgatggccgg gtgcgggtgc tcgacttcag cctcgcgcgc agctcgacac cggagccgga
      541 tcggcgcgcg ctcgacgagg cgctgacgac cggggacgtc gcggggatcc agtccgctca
      601 ggccgtcggc acgccggcgt acatgccacc ggagcaggcg ctcggggccg tcgccgacgg
      661 gcgcgcggac cagttcagct tctgcgtcgc gctgtacgag gcgctgtacg gccggcggcc
      721 gttcaccggg gagtcgacca ccgagctgat caaggacatc ctgcgcgaga acatccggcc
      781 gccggcctcg gcccgcctgc cgtcgtggct gacccgcgcg ctgctgcgtg gactgaggcc
      841 ggcgccggat cagcgctggc cgagcatggc cgcgctggtg gccgagctcg agcgcgaccg
      901 cggtcgctgg ctgcgactgg cctccgcgat cgcgctcgcg gcggtgctcg ccggcctcgt
      961 gctcggcgcg ctcgagctgc gcgcccgctg gcggcacgcg gcggtcgagc aggcggcggc
     1021 cgagcgcctc gcggtgctcg cggtcaacct cgaccagacc ctcgccgccg gccgccgcag
     1081 cgaggccgag gccgcgctcg cggcttttac cggcgcgccc gagcaccgcg acacgccggc
     1141 gctggcggcc gcctatctcg actggtcgcg gcgcatgcga gtcgcggccg acctcgacgc
     1201 ggcgcgcctg gccgccgcgc gggcctacgc ggcggccagc gacgagcccg gggtcgaggc
     1261 ggcgctcgag cagctggcgc tgctgttctg cgcgcagggg gcgtgggacg ccgcggcgct
     1321 gctcgtcgcc gacttgcggg tgcaccggcc ggcgctgctc gagcgcccgg tgtgggtcgc
     1381 gatcgcagcg caggcggccc gccgcagccg ggacctcggc gagttcgcca ggcaggccgc
     1441 gcgggcgaac gcgccgccgg agctcgccgt gctcgcgccg gtcgccgcgg ccctgtccca
     1501 tgggacagcc accgggcgcg tggccgcgcg cgccttcgcg gtccagggcg agccgacgct
     1561 ggccctcgtc gcgcgcatcg acgacgacgg cgccgagctc gtgcgcaagg acgtcgcgct
     1621 gagcccggtc ggccgcgtcg aggtgccggg cttcgcgatc gccgggctcg cgccgcggct
     1681 gcggccggac ggaccggcgc tgttgttggc gcaccgcagc gagccgccgg aggccgccgt
     1741 gctcgcgctc gcgtcggacg gcgcgcgcga gctgctgcgc ttctcgtcgg cgcgtccgct
     1801 cggcaacgcg acggccgacc tcgacggtga cggcaccccg agcctgtacc tcgggacagg
     1861 tccgtacgcg cgcgagctgc tggccctgac gccggacgcc gacgggacct tgcggctcga
     1921 gcacccgcac cccggcaccg acgccgcgca ttcggatctc caggacatgt tcaccgccga
     1981 cgtcgacggc gacggtcgca aggagctcgt cgtctcggcg ggcccgtgga acgccttcga
     2041 cctgcgggtg taccgggccg gcgcgaaggc ccccgcactc gagctgatgg cgcggcgaaa
     2101 attcggccac ctggtggcga tcggcgcgct ggcgggcagc gacggccgca cccggatcgt
     2161 cgccgccaag accgacccgt atccgagccg cttcgtgttc ccgcgcgaca ccccgaacgg
     2221 cgaccccgcc gggttctatg tgctcgagct gcgcggcgat cacatcgagg tcctcgcgca
     2281 ccacccgtgg ccggtcgcgc cgggccagcc gctcagcgtg tcgatgatgg gcgtcgggga
     2341 cgtcgacggc gacggcctcg tcgaccccgt cgtcacgctc agcctcggcg atcacagcga
     2401 gccgctcgtc gtcgtgtatc gccagctcgc cgacggtgcg ctcgcgccgg tcgagatcgc
     2461 ggggatcaat atgttgatgc tcgggcagtt cgacgacgac cccgccgtcg agctgctcgc
     2521 ccgcatgatg cccgacgacc catcggtgcc cgcgccgctg tgggtgctcg gggccggcga
     2581 cacgcccgtg cctccgctgg cgctgctgcg acccgagccg gcgagcgtgc ccgccggcgc
     2641 cgacccgctg gtcgcccgca cctgggcgcg cgccgagcag ctcgccagcc tcggcctgct
     2701 caccgaggcc gcgcagacga tctccgagct cgccagcctc gcgcagcgcc cggagatcgg
     2761 cgacgacgcg cgcctgcggg ccgccgagct gtaccgccgc gccgaccagt gtgcgccggc
     2821 caccgcgctc tacgggcagc tcggcgagcg ccacgccgtc gccgcgttgc agggcctcgc
     2881 cgcgtgtcac gagcgcgagg gtcgcttcgc cgaggcgctg gtcgacatcg acgcgctgcg
     2941 ggcccgcgcc gacctcgacc cggccgtgcg cgccgcggtc acccggcgcg ccgagcagct
     3001 gcgccccggc agcagcgaga cgccgatcga tctgcccttc gacggccacc tcgaccccgg
     3061 ctggcagatc gtcgacccga ccgcgctgcg ccaggacctc gtcaccggcg gcctggcgat
     3121 cgacgcgatc gccaaccgcg gcgttctcgc gcgcctgccg ctgcgctgtg acggcgaggc
     3181 gctggccgtg cgcgccgagc tgaccgtcga gcgcagcgag tgggcctccg gcttcgagat
     3241 cgcggtgcgc agcgccggcg gcgaggacct cgtcagcctc gacatcgcca ccggcggcgg
     3301 tggtgggatc tacagccgca cgatccgctg cggcggaccc gacgagaccc gtacgctaca
     3361 gatcctgcgc gacacggcgc ccgacacggt cgcgccgtat gtgctcgagg tggactaccg
     3421 gccggccctc ggtcgcagcg tctgcgccgt caccggcagc gacggcgtgc gccgctcgtg
     3481 ggccttcccc gcgcccgcga agctgccctc cggcgcgtgt gagctggtgc ttcgcgcgcg
     3541 tcacatcgtc ttcgacggct cgttctggac ccgcctgcgc ctcgacgcgc tgcgcgtgcg
     3601 tgggg
//
LOCUS       DASWNJ010002056         1871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_351891, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002056 DASWNJ010000000
VERSION     DASWNJ010002056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351891"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VGB85_34215"
     CDS             <1..221
                     /locus_tag="VGB85_34215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139210.1"
                     /translation="AASDTGRRYQTHAQLGTSIMLFARLSRDDRAFWFLGPATYVGHE
                     SEMPMAVTWRLHHPLPGDLFASFAAAVA"
     gene            264..>1871
                     /locus_tag="VGB85_34220"
     CDS             264..>1871
                     /locus_tag="VGB85_34220"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEY0139211.1"
                     /translation="MTRVQIIVSDIAALTVDAIVCQVDRRWAASGATAARVHEVAGPE
                     LAMMCDAFAPGEVAEVRRTLGFLLDAPGVLHVVEPDALSGEPEQLAACYRNCLAAARE
                     HHMRSIALPVPGIDGAPGRVALEIVHATITACIAATEDLPDDLVLVCPDDSTREAYER
                     LFAVRRVGAPSSVAQPVVRRLRGDIERLAAGSLAEREALARGLAALDWSSARHLDRLS
                     RGGPAMARAWALFALSGAAMHTQVRQVAGGQRLVSFHVKGPLAARLEAIEHALGLFVM
                     LADALDDAQIDLDVDGLHPLEAVDRLAAALGVRGWYDSRGMVHLTPPPEPPYPTAYAQ
                     GVRCQVVAVHCDRSTDFVRRIVDVRLRVAVDVAFPWAPLRPPQLQVIGAWDEADLGLA
                     GEATALAEQRYDGHLFEVRFAALRLATRCLRHVQLLVNIDLADAFETLVLRDLAPGAT
                     VSGALLRVSVVRGSSKGWNLEVATLDDATPPTVAEPTVLGISEDDAELAAITVSRTMG
                     DDRCVTWHLRWSRVAPNRLLELRIHAAR"
BASE COUNT          227 a          671 c          672 g          301 t
ORIGIN      
        1 gcgcggcgag cgatacaggc cggcgttatc agacccacgc gcagctcggg acctcgatca
       61 tgctgttcgc gcgtctgtcc cgggacgacc gggccttctg gttcctcggc cctgccacct
      121 acgtggggca cgagtccgag atgccgatgg ccgtcacctg gcgcctgcac catccactgc
      181 cgggcgacct gttcgcctcc ttcgccgccg cggtcgcctg agcagggcct gcgggcccat
      241 ggtagcctgt cggggccggc gtcatgactc gtgtgcagat cattgtcagt gatatcgcgg
      301 cgctgaccgt cgacgcgatc gtgtgccaag tcgatcgcag gtgggcggcg agcggcgcga
      361 cggcggcgcg cgtccatgag gtcgcgggcc ccgagctggc catgatgtgc gacgcgttcg
      421 cgccgggcga ggtcgccgag gtccgacgta cgcttggctt cctgctcgac gcgccgggcg
      481 tgcttcatgt cgtcgagccg gacgcgctgt ccggcgagcc cgagcagctc gccgcgtgtt
      541 atcggaattg tctggcggcg gcccgcgaac atcacatgcg atcgatcgcc ttgccggtgc
      601 cggggatcga cggcgctccc gggcgcgtcg ccctcgagat cgtgcatgcg acgatcaccg
      661 cgtgcatcgc cgcgaccgaa gatctaccgg acgacctcgt gctcgtgtgc ccggatgaca
      721 gcacacgaga ggcgtacgag cggctgttcg cggtgcgtcg cgtcggcgcg ccatcgtccg
      781 tcgcgcagcc ggtcgtccgc cgcctccgtg gcgacatcga gcgactcgcg gctggctctc
      841 tcgccgagcg cgaggcgctg gcgcgggggc tcgccgcgct cgactggtcg tccgcgcgac
      901 acctcgatcg actgtcccgg ggcggccccg cgatggcccg cgcgtgggcc ctgttcgcgc
      961 tctccggcgc cgccatgcac acgcaggtgc ggcaggtcgc cggtgggcag cgcctggtgt
     1021 cgtttcacgt gaaaggaccg ctggcggcca ggctcgaagc gatcgagcat gcgctcgggc
     1081 tgttcgtcat gctcgccgat gcgctggacg acgcgcagat cgacctcgat gtcgacggcc
     1141 tgcacccgct cgaggcggtc gaccgactgg ccgccgcgct cggtgttcgc ggatggtacg
     1201 attcacgcgg gatggtgcac ctcacgccgc cgcccgagcc gccgtacccg accgcatatg
     1261 cacagggcgt gcgctgccag gtcgtcgccg tgcattgcga ccggagcacc gacttcgtgc
     1321 gccgcatcgt cgatgtccgg ctgcgcgtcg cggtcgacgt cgcgtttccc tgggcgcccc
     1381 tgcggccccc gcagttgcag gtcatcggcg cctgggacga ggccgacctc ggcctcgccg
     1441 gcgaggcgac ggcgctcgcg gagcagcgtt acgacggtca cctgttcgag gtccgcttcg
     1501 ccgcgctgcg gctggccacg cgctgcctgc gtcatgtcca gctgctggtg aacatcgacc
     1561 tcgcggacgc gttcgagacg ctggtgctcc gcgacctcgc gccgggcgcg accgtctccg
     1621 gggcgctgct ccgcgtgtcc gtggtgcgag ggagctcgaa gggatggaac ctcgaggtcg
     1681 ccacgctgga cgacgccacc ccgccgacgg tcgccgagcc gaccgtgctc ggcatcagcg
     1741 aggacgacgc cgagctcgcg gcgatcacgg tgtcgcgcac gatgggggac gatcggtgcg
     1801 tcacctggca cctccggtgg tcccgtgtcg cgccgaatcg cctgctcgag ctacggatcc
     1861 acgccgcgcg c
//
LOCUS       DASWNJ010002057         3064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_352160, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002057 DASWNJ010000000
VERSION     DASWNJ010002057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352160"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VGB85_34225"
     CDS             complement(<1..316)
                     /locus_tag="VGB85_34225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139212.1"
                     /translation="MPTRPRWSEPVGPFRADQIREGDHYELSQGHAIHCMTAGGRHGS
                     SHILGSTVLDTDPAVAQQAGIDVGIAWNDGKNLRAPDIVVGMDLREPGWAREAPPLAG
                     ESA"
     gene            complement(367..753)
                     /locus_tag="VGB85_34230"
     CDS             complement(367..753)
                     /locus_tag="VGB85_34230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139213.1"
                     /translation="MSKRTAFVLLASLVSFSVTTPAQAACSDASNLCMEPVKGAKWEP
                     DKSVSAKQLKAKRKGPAGKLSVTIEDGRGSVFIDGRYAGTAPLSDVDLGPGKHDLQVR
                     DGNKILAEGVITIPKAASVSVTVKHS"
     gene            complement(792..1757)
                     /locus_tag="VGB85_34235"
     CDS             complement(792..1757)
                     /locus_tag="VGB85_34235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139214.1"
                     /translation="MSVSNLSRPRSRMLAVLLAVSMPAGALPAQAAPLAPLALFADAG
                     KVTAAQASGDRKPIQERAAGLLKSGNASDAAEVLSEEAGKRSDPVLYIDAAEAFQAAG
                     EESKSKSDLESAIERASIGLDILYFLQDARADPEWEIVESSDIPAEIRRGEKVIEASE
                     AAIAGLDKKTDAPAETGTEEKKPRKKAPKDGRGLIAAGSVLTLVGVGGIGLMGFGLAS
                     AGGAQKDIDALAEKVKSGAIDQATFDMQSEDIDAKGKRGNTLTYAGAAVGAVGLAAGI
                     ALLVVGVKKRKKYRAENGGGESDSTAMIVPSFGRDSVGLVLTGRF"
     gene            2026..2313
                     /locus_tag="VGB85_34240"
     CDS             2026..2313
                     /locus_tag="VGB85_34240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139215.1"
                     /translation="MLVTALFGCYDGPGELEPAPGQPGGLCIEPENVCDEVFWTCEPV
                     GGFCYNPGAPCQGVLCGGHGTCAEDPANGLPKCTCEPGYSNFEYSLLCLPP"
     gene            2357..>3064
                     /locus_tag="VGB85_34245"
     CDS             2357..>3064
                     /locus_tag="VGB85_34245"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEY0139216.1"
                     /translation="MASLMFRLLSRPLRLPAFLGPTPSPEDPQIFRFHGERAPIVSDV
                     VRRVVQHYAGQPEKLLHVLNEAANAEVRRLASQGDMEASDALGSWKRLVRRLGKMDED
                     ERREALREKVRDMTEDIAGNFDPRVYGLATRLVPNVLTAIMNPRAMPQQLLSGFRPLD
                     GLITTEGPVDKLRRLARTGTLVLVPTHSSNLDSIALGYVLMRERLPPMVYGAGKNLFS
                     NPLISFFMHNLGASRVDR"
BASE COUNT          393 a         1025 c         1146 g          500 t
ORIGIN      
        1 cggcggactc gcccgcgagc ggcggcgcct cgcgggccca gccgggctcg cgcaggtcca
       61 tgccgacgac gatgtccggc gcgcgcaggt tcttgccgtc gttccaggcg atgccgacgt
      121 cgatgccggc ctgctgcgcg accgcggggt cggtgtcgag gacggtgctg ccgaggatgt
      181 gcgagctgcc gtgccggccg cccgcggtca tgcagtggat cgcgtggccc tgcgagagct
      241 cgtagtggtc gccctcgcgg atctggtcgg cccggaaggg accgacgggc tcgctccagc
      301 ggggtcgggt cggcatcgac cgaggatagc gcgcgccggg gcgcgtggcc aggcggcgcg
      361 ggcgcctcag gagtgcttga cggtgacgct gacgctcgcg gccttgggga tcgtgatgac
      421 gccctcggcg aggatcttgt tgccgtcgcg gacctggagg tcgtgcttgc cggggccgag
      481 gtcgacgtcg ctgagcgggg cggtgccggc gtagcgaccg tcgatgaaca cgctgccgcg
      541 gccgtcctcg atcgtcacgc tgagcttgcc ggccgggccc ttgcgcttgg ccttgagctg
      601 cttggccgac accgacttgt cgggctccca cttggcgccc ttgacgggct ccatgcacag
      661 gttgctggcg tcgctgcaag cggcctgcgc gggcgtcgtc acggagaagg acacgagcga
      721 ggcgaggagg acgaaggcgg tgcgcttgga catggctttt ataaattcct ggtcgaggtg
      781 tcgggtgcgg atcagaagcg gccggtgagg accaggccga cggagtcacg cccgaacgag
      841 ggcacgatca tcgcggtgga gtcggactcg ccgccgccgt tctccgcgcg gtacttcttg
      901 cgcttcttga cgccgacgac gagcagggcg atgccggcgg cgaggccgac cgcgccgacg
      961 gccgcgccgg cgtaggtcag ggtgttgccg cgcttgccct tggcgtcgat gtcttcgctc
     1021 tgcatgtcga aggtggcctg atcgatcgcg ccacttttga ccttctcggc gagcgcgtcg
     1081 atgtcctttt gggcgccgcc ggccgaggcg aggccgaagc ccatcaggcc gatgccgccg
     1141 acgccgacga gggtcagcac cgagccggcg gcgatcaggc cgcgaccgtc cttgggggcc
     1201 ttcttgcgcg gcttcttctc ttcggtgccg gtctcggcgg gggcgtcggt cttcttgtcg
     1261 aggccggcga tcgcggcctc ggaggcctcg atgaccttct cgccgcggcg gatctcggcg
     1321 gggatgtcac tgctctcgac gatctcccac tccgggtcgg cgcgggcgtc ctgcaggaag
     1381 tagaggatgt cgaggccgat gctcgcgcgc tcgatcgccg actcgaggtc ggacttcgac
     1441 ttgctctcct cgcccgcggc ctggaacgcc tccgcggcgt cgatgtagag cacggggtcg
     1501 ctgcgcttgc cggcctcctc gctcaggacc tcggcggcgt cgctcgcgtt gccggacttg
     1561 agcagacccg cggcccgctc ctggatcggc ttgcgatcgc cgctcgcctg cgccgcggtg
     1621 accttgccgg catcggcgaa cagcgccagc ggagcgagcg gggcagcctg cgccggcaag
     1681 gcaccggcgg gcatcgacac ggccagcaat accgcgagca tgcgcgagcg gggacgcgag
     1741 agattcgaga cggacatcgc cgccacgcta ccagcgtcgg gcctgccacg acaaccaccc
     1801 gccatcgtgg ggcgtggccg ggagccccca aagtttcgcc ggcgccgcgt cggcgcgagg
     1861 gtctgcgaag tatgtccctc agatccctgg ccgatgaggt ggatgaggtg gatgcgtcac
     1921 tgcggtctac ttcccccgcg ccgggctttc gcctacagtg cagtcgtgcg accgcgcagc
     1981 cttcggcccc cagcctggtg tagatccctc gcctgcgggt cgctggtgct cgtcactgcg
     2041 ctgttcggct gttacgacgg accgggcgag ctcgagccgg cgccgggcca gccgggcggg
     2101 ctgtgcatcg agccggagaa cgtctgcgac gaggtgttct ggacctgcga gccggtcggc
     2161 ggcttctgtt acaaccctgg cgcgccgtgc cagggggtgc tgtgcggggg gcatggcacg
     2221 tgcgcggagg acccggcgaa cgggctgccg aagtgcacct gcgagccggg ctacagcaac
     2281 ttcgagtact cgctgctgtg cttgccgccg tgagcgggtg tggcggggcc ccggctgcga
     2341 tatgctcgcg ccggccatgg cttcgctgat gtttcgtctg ctcagtcgtc ccctgcgctt
     2401 gccggctttc ctcggcccga cgccgagccc ggaggacccg cagatcttcc gcttccacgg
     2461 ggagcgggcg ccgatcgtca gcgacgtggt gcggcgggtg gtccagcact acgccgggca
     2521 gccggagaag ctgctgcacg tgctcaacga ggcggccaac gccgaggtgc ggcggctggc
     2581 gagtcagggc gacatggagg cgagcgacgc gctcggctcg tggaagcggc tcgtgcggcg
     2641 gctcggcaag atggacgagg acgagcggcg cgaggcgctg cgcgagaagg tccgcgacat
     2701 gaccgaggac atcgcgggga acttcgaccc gcgggtgtac ggcctggcga cgcgcctggt
     2761 gcccaacgtg ctcacggcga tcatgaaccc gcgggcgatg ccgcagcaac tgctctccgg
     2821 gtttcgtccg ctcgacgggc tgatcaccac ggaggggccg gtcgacaagc tgcgccggct
     2881 ggcgcggacc ggcacgctgg tgctggtgcc gacgcactcg tcgaacctcg actcgatcgc
     2941 gctcggctac gtgctgatgc gcgagcgcct gccgccgatg gtctacggcg ccggcaagaa
     3001 cctgttctcg aacccgctga tcagcttctt catgcataac ctcggcgcct ctcgcgtcga
     3061 ccgc
//
LOCUS       DASWNJ010002058         6971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_352354, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002058 DASWNJ010000000
VERSION     DASWNJ010002058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 6971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6971
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352354"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VGB85_34250"
     CDS             complement(<1..355)
                     /locus_tag="VGB85_34250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139217.1"
                     /translation="MRDRNFQRDNDDFGSNQDRGSRSQGGSQGGHGGSQNFGGSQGGS
                     QGGYGGGSQSFGGGSQGGYGGSQGGHGGSQNYGGGSQGGYGGSQGGSQGGYGGSQNFG
                     GGSQGGYGGGSQGGYG"
     gene            703..1500
                     /locus_tag="VGB85_34255"
     CDS             703..1500
                     /locus_tag="VGB85_34255"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY0139218.1"
                     /translation="MATHVIYKLGPFEVPRLPGERHVRVYLPTERTDIEPLPVLYMFD
                     GQNIFHDSPSYSGGWYLHHAVHDLTAAGKTAPVVVGIDHGNENRVHELSPFTCEESRG
                     ELHHMLRWIVRELRPRINKEFHVSQDVGNTAIGGSSMGGLAALYGHFRRPDVFGAALC
                     MSPSLWFAQHKIYEHLAGQPLPWSSRIYIDAGAHEGDMLHDAERLVRDLRGRGYDDTS
                     LMFLADPYGKHSEQDWRKRAPSAIEFLFSGKHGQYAGSAREDDQEAA"
     gene            1556..2050
                     /locus_tag="VGB85_34260"
     CDS             1556..2050
                     /locus_tag="VGB85_34260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139219.1"
                     /translation="MSKRDGCPPTPPPGGPVLVRRAAIVLSLGALACGDDKSDNGDST
                     TGTTTAPMTTAPMANPTTGDSTTASDTSTTTTTTTTSPTTTAPMPNPTDSASTLPPMP
                     GPETTTTDGSATADTDMDTDMDTGTTAIPPMPPPMPNPDTDTEGTTAVPPMPPPMPNP
                     SGET"
     gene            complement(2109..2756)
                     /locus_tag="VGB85_34265"
     CDS             complement(2109..2756)
                     /locus_tag="VGB85_34265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139220.1"
                     /translation="MRPRLVVGLVLAACAGRPSTDPGDNKEPLPSTNAPAAFPFATRP
                     PVRREHIQVKATDHGIMGSYGPMTHVLFTAEVARLLRTDGECIYFVETSAPVLRVLCP
                     RSDSRSRKILRLDPIDHPSFRSRDPLAFLQRQEDLSVDSGVLCLDLHDRPSQPSATYN
                     IRVDLVRGTHVRHLVEDLARDSDGLAREPIRPRLCTAHGPSAHESSDGTYLSLDL"
     gene            3056..3439
                     /locus_tag="VGB85_34270"
     CDS             3056..3439
                     /locus_tag="VGB85_34270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139221.1"
                     /translation="MRTSLSLALLLAFTPACDSPDDEEALQALDAEAELQAAEDLQGS
                     PASLALDVITSDPQPVAAQPNQLCRTNSGAILYTSPTSGIYVPPNALVRILDYAGPYH
                     YLARYDGTVGQLERSRINQSSCYYQ"
     gene            complement(3673..3894)
                     /locus_tag="VGB85_34275"
     CDS             complement(3673..3894)
                     /locus_tag="VGB85_34275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139222.1"
                     /translation="MAQKFVRPGYVPALNLRAAQGLQRASQAGAESLVFGEREVAPGG
                     LELGRAQGRCDAQIRDFRRHTMTEQYGSQ"
     gene            3919..4599
                     /locus_tag="VGB85_34280"
     CDS             3919..4599
                     /locus_tag="VGB85_34280"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEY0139223.1"
                     /translation="MFTDIVPHWPAYRRWSPEHLRTHYGHVELEIEAGRAADATPDLN
                     FERHRGTATMAEFVERVLAAGETNDLYLIANNRNLARPGLQPLLEDIELPAGYFDPAP
                     GRAARCGAFWFGPAGTVTALHHDTSNILFFQVLGRKRFRLYPPDEPALLTGARGVYND
                     LDPEHDAAVLAAVPGFDLVLAPGEALFLPIGWWHHVRALDLSISVAFNHFNWPNDFDW
                     YRPGTPRR"
     gene            complement(4616..4882)
                     /locus_tag="VGB85_34285"
     CDS             complement(4616..4882)
                     /locus_tag="VGB85_34285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139224.1"
                     /translation="MRDEFVGRANKIRAERDRKLEQARGLQRKRRVLDHLGTARNYPP
                     HQLSVDHPTHVSRSRRANTPLLHITAPNPEPATLIGRDQSIGTR"
     gene            complement(4994..5680)
                     /locus_tag="VGB85_34290"
     CDS             complement(4994..5680)
                     /locus_tag="VGB85_34290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139225.1"
                     /translation="MLGACTLQKDLLTTGDPDSTDDGAATADDESATGNIGNTGGPGS
                     DATGDTSTAGDTASGSEGSSDTTPAEMCPPDGIDMTISSGVGCDAEMPDYIGKPLPTN
                     LSANCAASPEKTELWESEAEMTGCIVGQWLACPGFTVSGVGSGVGLEFTADGKVIRLL
                     LDENCEVVRGTGIDHVGTWEYGDVGTGDGPNGLRYTWDPDQNGWLTTAPLVTTNPEQM
                     RAHDGRLARP"
     gene            6087..6350
                     /locus_tag="VGB85_34295"
     CDS             6087..6350
                     /locus_tag="VGB85_34295"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEY0139226.1"
                     /translation="MLANLTLPAGYFDPSPIRAARCGAFWFGPAGTVTALHHDTSNIL
                     FFQVVGRKRYRLYPRASPRCWPTPAASTTTSTPSAAPVTDGPR"
     gene            complement(6364..>6971)
                     /locus_tag="VGB85_34300"
     CDS             complement(6364..>6971)
                     /locus_tag="VGB85_34300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139227.1"
                     /translation="AATADDESATGNIGNTGGPGSDATGDTASGSEGPPSDTTPVEMC
                     PPDGIDMTISSGVGCDAEMPDYIGKPLPANLSANCAASAEKTESWESEVEMTACIVGQ
                     WLLCPGFPVYGVGSGVGLEFTPDGKMIRLLLDENCEVVRGTGIDHVGTWQYGDVGTGS
                     GPNGLLYTFDQNENSWWTTAPLVTTNPEQMRTNDGGRLARP"
BASE COUNT         1126 a         2521 c         2181 g         1143 t
ORIGIN      
        1 caccgtagcc gccctgcgaa ccaccgccgt agccgccctg cgacccgccg ccgaagttct
       61 gcgacccacc gtagccgccc tgcgagccac cctgcgaccc accatagccg ccctgcgacc
      121 caccaccgta gttctgcgaa cccccatggc cgccctgcga accgccgtag cctccttgcg
      181 acccaccgcc gaagctctgc gacccgccgc cgtacccacc ttgcgaaccg ccctgcgatc
      241 cgccgaagtt ctgtgagccg ccgtggccac cttgcgagcc gccttgcgat cgcgaaccgc
      301 ggtcctggtt cgagccaaag tcatcgttgt cgcgctggaa gtttcggtct ctcatggtcg
      361 tctccttcag attcacgctc tcgcgcatta cgcgcggttg tggagggtgc tgtccgcgga
      421 gagcctgcat caacgcgggc tcgtgactcg ctggtgaaaa ccatcaccgc gactgcgcct
      481 cgatgaacac cgtcctcgca acggccgcgc ccgggtgcgc agttgccggc gtcccacacc
      541 gcccgggaaa ccccctgtga gcgccctgcg aggcccgcag accgcatgcg acgcaagtcg
      601 aacattggcc gaaaaacgcg tctgttggat cccgagcacg tggtgtgatg ggcgtccatg
      661 tgtccgcttg cgtgctgatg tgcccgcgct aaaaggccga acatggccac gcacgtcatt
      721 tacaagctcg gtcccttcga ggtcccgcgg ctcccgggcg agcgccacgt tcgcgtgtat
      781 ctaccgaccg agcgcaccga catcgagccc ttgccggtgc tctacatgtt cgacgggcag
      841 aacatctttc atgatagccc ctcgtactcc ggtggttggt atctgcacca cgcggtgcac
      901 gacctgacgg ccgccggcaa gacggcgccg gtggtcgtcg gtatcgacca cggcaacgag
      961 aaccgggtgc acgagctctc gcccttcacg tgtgaggaga gccgcggcga gctccaccac
     1021 atgttgcgct ggatcgtgcg agagctgcgg ccgcggatca acaaggagtt ccacgtcagc
     1081 caggacgtcg gcaacaccgc gatcggcggc tcctcgatgg gcggcctcgc ggccctctac
     1141 ggtcactttc gccggcccga cgtcttcggc gccgcgctgt gcatgtcgcc ctcgctgtgg
     1201 ttcgcgcagc acaagatcta cgagcacctc gccggtcagc cgctcccgtg gtcctcgcgg
     1261 atctacatcg acgccggcgc gcacgagggt gacatgctcc acgacgccga gcgcctggtc
     1321 cgcgacctgc gcggccgcgg ctacgacgat accagcctga tgttcctcgc cgacccgtac
     1381 ggaaagcaca gcgagcagga ctggcgcaag cgtgccccct ccgcgatcga gttcctgttc
     1441 tcgggcaagc acggccagta cgccggcagc gcccgcgaag acgaccagga agccgcctga
     1501 ccccacccgg tcatgcgcgc ttgacagccg ccgcgccccg gcgtcccatg gtcgcatgag
     1561 caagcgcgac ggctgcccgc cgaccccacc gcccggcggc cctgtcctgg tccgccgcgc
     1621 cgcgatcgtc ctcagcctcg gcgccctcgc ctgcggcgac gacaagagcg acaacggcga
     1681 cagcaccact ggcacgacga ccgcgccgat gaccaccgcg ccgatggcca acccgaccac
     1741 cggcgacagc accaccgcga gcgacacgtc gacgaccacc accaccacga ccacctcgcc
     1801 gaccaccacg gccccgatgc cgaacccgac cgactccgcc tcgacgctcc cgccgatgcc
     1861 gggcccagag acgaccacca ccgacggcag cgcgaccgcg gacacggaca tggacacgga
     1921 catggacacc ggcacgaccg ccatcccgcc gatgcccccg ccgatgccga acccggacac
     1981 cgataccgaa ggcaccaccg cggtcccccc gatgccccca ccgatgccga accccagcgg
     2041 cgagacctga tcgcgcccgc gcgcctgcgc cggcccgcgc gaccccctcg tggacgtgcg
     2101 ctctaccgtt acagatctag cgacaggtag gtcccgtccg aggattcgtg cgcggacggg
     2161 ccatgcgcgg tgcacagacg cgggcggatg ggttcgcgcg ccaggccatc actgtcgcga
     2221 gcgagatctt cgacgaggtg gcgtacatgg gttccgcgaa cgagatcgac gcggatgttg
     2281 taggtcgcgc tcggctgact cggtcgatcg tggaggtcga ggcagaggac gccgctgtcg
     2341 acgctcaggt cctcttgccg ctgcaagaac gcgagcgggt cgcgcgaccg gaaactcgga
     2401 tgatcgatcg ggtcgagccg aaggatcttg cgggatctgc tgtcggagcg ggggcagagg
     2461 acgcgaagca ccggggcgga ggtctcgacg aagtagatgc actcgccgtc cgtacgcaac
     2521 aatcgggcga cctcggccgt gaacagaaca tgggtcattg gaccatacga gcccatgata
     2581 ccgtgatcgg tcgccttgac ctgaatatgc tcgcgtctga ccggtggtcg cgtggcgaac
     2641 ggaaatgcag caggtgcatt tgtcgaaggc agcggctcct tgttgtcgcc agggtcggtc
     2701 gaaggccggc ctgcgcaggc ggccaggacg aggccgacga cgagccgcgg gcgcatggaa
     2761 cagagggtag cacggcatca cgcacatcgt gaggcccgcc gggctccgaa gtgcgtccgc
     2821 tcggcggcgt cggcctctcg ggtccaccga gcgctatgcg cgatcctgaa gtgcgccgca
     2881 ggctaccgac atcgaggccg cgcattcgct cgggtcgagg atgtgaatgc aatagaatgt
     2941 tgatggtttg ttttgttgcc attctcggtt atctaaatca aaaagatcga tccgcgatca
     3001 acggccggtg atctggagca tgatcgatct gtcttgctgg aggtaccaat aacgtatgcg
     3061 gacatctctc tcactcgctc tcttgctcgc cttcaccccg gcttgtgact ctcccgatga
     3121 cgaggaagcg cttcaagcgc tcgacgcgga ggccgagctc caggcggcgg aggatctcca
     3181 gggatcacct gccagcctcg cgctggatgt catcaccagt gacccgcagc ccgtcgctgc
     3241 ccagccgaac cagctgtgcc gcaccaacag cggcgccatt ctctacacga gcccgacctc
     3301 ggggatctac gtcccgccga acgcgctggt ccgcatcctc gactacgcag gaccctacca
     3361 ctacctcgcg cgctacgatg gtacggtggg tcagctggag cgcagcagga tcaatcagtc
     3421 gagctgctac taccagtgac cgtcggccca ggggcgcgcc ggaacgcggt cgctcgcggc
     3481 ccggcgccct cacgggagcg atcccggacg ggcttcggcc gccgcggcgt cgtggtaaca
     3541 catgacgcct gagccatgcg agcacgcctg caccgagtca cgagacgtca tccgggccgc
     3601 agcaaatggg ccatcgccag accgtggcgc gtgtggctgg ccgacaacct cctgcgtggc
     3661 gcctcgcccg ggtcattgcg agccgtactg ctcagtcatg gtgtgccgcc gaaagtcgcg
     3721 gatctgggcg tcgcagcggc cttgcgcgcg cccgagctcg aggccgcccg gggccacctc
     3781 gcgctcgccg aacacgagag actcggcgcc cgcctgcgac gcacgctgca ggccctgcgc
     3841 ggcccggaga ttgagcgccg gaacgtaccc gggcctgacg aatttctgcg ccattattac
     3901 agcaccggga cgcccgtcgt gttcactgac atcgtcccgc actggcccgc ctatcgccgg
     3961 tggagtcccg aacacctgcg cacgcactat ggacacgtcg agctcgagat cgaagccggc
     4021 cgcgccgccg acgcgacccc cgacctcaac ttcgagcggc accgcggcac cgccacgatg
     4081 gccgagttcg tcgaacgcgt gctcgcagcc ggcgagacca atgatctcta cctgatcgca
     4141 aacaaccgca acctcgcccg cccgggcctg cagccgttgc tcgaggacat cgagctgccc
     4201 gccgggtact tcgacccggc accaggccgc gcggcccgct gcggtgcctt ctggttcggt
     4261 ccggccggca ccgtcaccgc gcttcatcac gacacttcga atatcctgtt ttttcaggtc
     4321 ctcgggcgca aacgctttcg cctctatccg cccgatgagc cggccctgct gaccggagcg
     4381 cgcggcgtgt acaatgacct cgatccggag cacgacgccg cggtgctggc tgcggtcccg
     4441 gggttcgacc tcgtgctcgc tcctggcgag gccctgttcc tgccgatcgg ctggtggcac
     4501 cacgtgcgcg cgctcgacct cagcatcagc gtcgccttca accacttcaa ttggcccaac
     4561 gatttcgact ggtaccgccc cggcacgccg cgaaggtgag gaactggact cgccgtcatc
     4621 gggtgccgat ggactgatcg cggccgatca gagtcgcggg ctccgggttt ggtgccgtta
     4681 tatgcaaaag tggtgtattg gctcggcgtg atcgggacac gtgggtgggg tgatcaacgg
     4741 agagctggtg aggcggatag ttacgcgcgg taccgaggtg gtccagtact cggcgcttgc
     4801 gttggagccc gcgagcctgc tcgagcttgc ggtcgcgctc ggcccggatc ttgttcgcgc
     4861 ggccgacgaa ctcgtcgcgc atggtgacgt ggccgatggc gctgtgagac gtcgagcagg
     4921 gcctctccga cgaaggccgg acagcggacg tcggccacgg tgaccgacgc gctgcgatga
     4981 tgcgcggcgc gactcagggc cgggctagac gtccgtcatg ggcgcgcatc tgctccgggt
     5041 tcgtcgtgac gagcggcgca gtcgtcaacc agccgttttg gtccggatcc caggtgtacc
     5101 gcaatccatt gggcccgtcg ccggtcccca cgtcgccgta ctcccacgtg ccgacatgat
     5161 cgatgcccgt gccgcgcacg acctcgcaat tctcgtcgag caacagccgg atcaccttgc
     5221 cgtccgcggt gaattccagc ccgacgccgc tgccgacgcc ggacaccgtg aatcccgggc
     5281 atgccaacca ttgcccgacg atgcagcccg tcatctccgc ctccgactcc cagagctcgg
     5341 tcttctccgg cgacgccgcg caattcgccg aaaggttcgt cggcagcggc ttgccgatgt
     5401 agtcgggcat ttcggcgtcg cagcccacgc cgctcgagat cgtcatgtcg atgccgtccg
     5461 gagggcacat ctctgccggt gtggtgtcgg aggacccctc ggatccactc gcggtatcgc
     5521 ccgccgtgct ggtatcgccc gtcgcgtcgg accccggccc gccggtattg ccgatattgc
     5581 ccgtcgcgga ctcatcgtcg gcggtcgccg cgccgtcgtc ggtcgaatcg ggatcgcccg
     5641 tggtcagcag atccttctgg agcgtgcagg cgccgagcaa ggggacgagg agcaaggagt
     5701 aagagaaaat ggacatgccc cctgcttctc cggccgctcg ggttcctgtc tcccgccgag
     5761 aaatatttct ccgcgaaggc gccgctttcg cggagggcag gaccgagcca cagcgtcgct
     5821 gggagtggcc gcggtcgatg ccgcgcggtc gcgctcgcgc gacccgacat acgacggcct
     5881 ggccatgggc gtcgcctcca ttcgactccg ctgagcgccg taaggctgcg ttgtcggcgg
     5941 ctagatcccc gtccctggcc cgatcttgtc gcgattttgg cctgtctcga gaatattccc
     6001 tgaagtctgg ctcggaggcc ctactgcgtg gacatattcc cctgatcgcc aacaaccgca
     6061 acctcgcccg ccccagcctg caagcattgc tcgccaacct gacattgccc gccggctact
     6121 tcgacccctc tccgatccgc gcggcccgct gcggcgcctt ctggttcggc cccgccggca
     6181 cagtcaccgc cctgcatcac gacacttcca acatcttgtt cttccaggtc gtcggccgca
     6241 aacgctatcg cctgtatccc cgggcgagcc cacgctgctg gcccacgccc gcggcgtcta
     6301 caacgacctc gaccccgagc gcagccccgg tgaccgacgg gccgcgatga tgcgcggcgc
     6361 ggctcagggc cgggctagac gtccgccgtc attggtgcgc atctgctccg ggttcgtcgt
     6421 gacgagcggc gcagtggtcc accagctgtt ttcgttctga tcaaaggtgt acagcaatcc
     6481 attgggcccg ctgccggtcc ccacgtcgcc gtactgccac gtgccgacat gatcgatgcc
     6541 cgtgccgcgc acgacctcgc aattctcgtc gagcaacagc cggatcatct tgccgtccgg
     6601 agtgaattcc agcccgacgc cgctgccgac gccgtacacc gggaatcctg ggcatagcaa
     6661 ccattgcccc acgatgcagg ccgtcatctc cacttccgac tcccaggact cggtcttctc
     6721 cgccgacgcc gcgcaattcg ccgagaggtt cgccggcagc ggcttgccga tgtagtcggg
     6781 catttcggcg tcgcagccca cgccgctcga gattgtcatg tcgatgccgt ccggagggca
     6841 catctctacc ggtgtggtgt cggagggcgg cccctcggat ccactcgcgg tatcgcccgt
     6901 cgcgtcggac cccggcccgc cggtattgcc gatattgccc gtcgcggact catcgtcggc
     6961 ggtcgccgcg c
//
LOCUS       DASWNJ010002059         2263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_352460, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002059 DASWNJ010000000
VERSION     DASWNJ010002059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352460"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..500)
                     /locus_tag="VGB85_34305"
     CDS             complement(162..500)
                     /locus_tag="VGB85_34305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY0139228.1"
                     /translation="MPKITFIGPDGTKIVEAARGQTLLELAEDNDVKMGSACGGVCAC
                     SSCHCWIREGLRTLPEMTDSESDRLDMGFDVRPYSRLGCQVEVADADLTVELTQESIQ
                     TWRNEHPGKA"
     gene            complement(523..>2263)
                     /gene="hscA"
                     /locus_tag="VGB85_34310"
     CDS             complement(523..>2263)
                     /gene="hscA"
                     /locus_tag="VGB85_34310"
                     /inference="COORDINATES: protein motif:HMM:NF003520.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEY0139229.1"
                     /translation="SSVKRFMGRGAADVRFPHPYELTGEGGVLRIEVGDAGQPRQVSP
                     VEVSAEILRVLKLRAEAELGGPVDGAVITVPAYFDDAQRQATRDAGRIAGLKVYRLLA
                     EPTAAALAYGLDQRDHHDHIGQGDANHGLFAVYDLGGGTFDISVLRLHEGVFQVLATG
                     GDSALGGDDFDRALAAELLRLAGLETPTHAQHDATRVAARRLKERLSVDEAASISVPA
                     IGLADAEVTRATFEALIEPLAQRTLRACRNALKDAGVRAQELDGVVLVGGSTRVPRIR
                     TLVEQTFGRAPLTDIDPDEVVAHGAAIQADILSGSERPGVTLLDVVPLSLGLETMGGI
                     VEKIIPRNATIPISARQVFTNYSEQQTGMVIHVLQGERELARDNRSLARFDLKGIPRL
                     PPSMARVEVTFQLDADALLTVTARELMTGVKQSVEVKPTHGLAADEVDRLVIESLDHA
                     GEDYSARSVAEARVELGRVILAVQGPLAEVGHVEALISADERLAIEAALRDADTAMAD
                     PTADGDALTAVRQRLEKVSEAFAARRMQRALRAGMSGKSLAEIEAALADEGLDAKRGG
                     HSAELLPTDDPQT"
BASE COUNT          291 a          783 c          852 g          337 t
ORIGIN      
        1 aggctcgggc gatcgagacg gtcctgcgtg cccgcggcct gacgatcgac gagcgcctgc
       61 tgacgcgcct gcgaagctgc cgcgaccctg ccgtgctcag ccgctggctg gtccagtcgg
      121 cgaccgcgac gaccgccgag gcagcgctcg actgaccgcg ctcaggcctt gccggggtgc
      181 tcgttacgcc aggtctggat cgactcctgc gtgagctcga cggtgaggtc ggcgtccgcg
      241 acctcgacct ggcagccgag gcggctgtag gggcgcacgt cgaagcccat gtcgaggcgg
      301 tcgctctcgc tgtcggtcat ctccggcagg gtcctcaggc cctcgcggat ccagcagtga
      361 cacgacgagc aggcgcacac gcccccgcag gccgagccca tcttgacgtc gttgtcctcg
      421 gcgagctcga gcagggtctg accgcgcgcg gcctcgacga tcttggtccc gtcgggaccg
      481 atgaaggtga tcttcggcat gggaaagcga gtcgtgcggg tctcaggtct gtgggtcgtc
      541 ggtcggcagc agctcggcgc tgtggccccc gcgcttggcg tcgaggccct cgtcggcgag
      601 cgcggcctcg atctcggcga ggctcttgcc ggacatgccg gcacgcagcg cccgttgcat
      661 ccggcgcgcg gcgaaggcct cgctgacttt ttccaggcgc tgccgcactg ccgtcagcgc
      721 gtcgccgtcc gcggtcggat cggccatcgc ggtgtcggcg tcgcgcaacg cggcctcgat
      781 cgcgaggcgc tcgtccgcgg agatcaacgc ctcgacgtgg ccgacctcgg ccagcgggcc
      841 ctgcaccgcc aggatcaccc ggccgagctc gacgcgggcc tcggcgacgc tacgcgcgct
      901 gtagtcctcg ccggcgtggt cgaggctctc gatcacgagg cggtcgacct cgtcggcggc
      961 gaggccgtgg gtcggcttga cctcgacgga ctgcttcacg ccggtcatca gctcgcgcgc
     1021 ggtgaccgtc agcagcgcgt cggcgtcgag ttggaaggtg acctcgacgc gggccatcga
     1081 cggcggcagg cgcgggatgc ccttgaggtc gaagcgggcg aggctgcggt tgtcgcgggc
     1141 cagctcgcgc tcgccttgca gcacgtggat gaccatgccg gtctgctgct cggagtagtt
     1201 ggtgaacacc tggcgcgcgg agatcgggat cgtcgcgttg cgcgggatga tcttctcgac
     1261 gatgccgccc atcgtctcga ggccgaggct cagcgggacg acgtcgagca gcgtcacacc
     1321 ggggcgctcg gagccggaca agatgtcggc ctggatcgcc gcgccgtggg cgacgacctc
     1381 gtcggggtcg atgtccgtca ggggcgcgcg gccaaaggtc tgctcgacga gcgttcgtat
     1441 ccgcgggacg cgggtcgagc cgccgacgag caccacgccg tcgagctcct gcgcgcgcac
     1501 gccggcgtcc ttgagcgcgt tgcgacacgc tcgcagggtc cgctgggcca gcggctcgat
     1561 cagcgcctcg aaggtcgcgc gggtgacctc ggcgtcggcg aggccgatcg cgggcacgga
     1621 gatcgaagcg gcctcgtcga cgctgaggcg ctccttgagg cggcgcgccg cgacgcgggt
     1681 cgcgtcgtgc tgcgcgtgcg tgggggtctc gaggccggcg agccgcagca actccgccgc
     1741 gagggcgcgg tcgaagtcgt caccgccgag cgcgctgtcg cctccggtgg cgagcacctg
     1801 gaacacgccc tcgtgcaggc gcagcacgct gatgtcgaag gtgccgccgc cgaggtcgta
     1861 gacggcgaac aggccgtggt tggcgtcacc ctggccgatg tgatcgtggt gatcgcgttg
     1921 atcgaggccg taggcgagcg cggccgcggt cggctcggcg agcaacctgt acaccttgag
     1981 cccggcgatg cgaccggcgt cgcgggtcgc ctggcgctgg gcgtcgtcga agtacgcggg
     2041 caccgtgatc acggcgccgt cgaccggacc gccgagctcg gcctcggcgc gtagcttgag
     2101 cacgcgcaag atctcggccg agacctcgac gggcgagacc tggcgcggct gcccggcgtc
     2161 gccgacctcg atgcgcagca cgccgccctc ccccgtcagc tcgtacgggt gcgggaagcg
     2221 gacgtcggcc gcgccgcggc ccatgaagcg cttgaccgac gag
//
LOCUS       DASWNJ010002060         1607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_352750, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002060 DASWNJ010000000
VERSION     DASWNJ010002060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352750"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VGB85_34315"
     CDS             <1..765
                     /locus_tag="VGB85_34315"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0139230.1"
                     /translation="VLESELFGHEKGAYTGASQPRAGVFERADGGTLLLDEIGEVDLE
                     FQAKLLRVLQTREVLPVGGAKPRTIDVRLVAATNRDLRAEVAAGRFREDLYFRLAVIP
                     IRLPPLRERPEDILPLARHFLARWSAELGRRIEGWTPEVEAWLLAHPWPGNVRELENL
                     VERAAVLAREDQISLADLLLDSAGPGGAGQSATAGGEDAELPLQQYLDRAAERRIRGA
                     LARCEGRRAEAARSLGVERTTLYRLMKKFGISVDGE"
     gene            823..1443
                     /locus_tag="VGB85_34320"
     CDS             823..1443
                     /locus_tag="VGB85_34320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139231.1"
                     /translation="MRTLLLWLGLGAGAALAVACDDADSVDCECAEVGCFAPMCTRLA
                     FVLAEPVSPKFGGVSAADQMCAQQAQAAGLPGTYYAWLGHSGGGPADRFSRASVPYTL
                     PDGTLLAADWDALTREGPDAAIDMTAAGKRVEAEDDARVWTGTNEAGDANTYNNASNY
                     CSDWTRNVIGEFAVVGFLRDRSKIGAWALGNLVPCTGKGYVYCFQQ"
BASE COUNT          239 a          507 c          615 g          246 t
ORIGIN      
        1 gtgctcgaga gcgagctttt cgggcacgag aagggcgcat acacgggggc gagccagccg
       61 cgcgccggtg tgttcgagcg ggccgacggc ggcacgctgc tgctcgacga gatcggtgag
      121 gtcgacctcg agtttcaggc caagctcctg cgcgtgctcc agacccgcga ggtgctgccg
      181 gtcggcggcg cgaagccgcg gacgatcgac gtgcgcctgg tcgccgcgac gaaccgcgat
      241 ctgcgggccg aggtcgcggc gggccgattc cgcgaggacc tgtactttcg cctcgcggtg
      301 atcccgatcc gcctgccgcc gctgcgcgag cggcccgagg acatcctgcc tctggcccgg
      361 cacttcctgg cccgctggag cgcggagctc gggcgcagga tcgaaggctg gacgccggag
      421 gtcgaggcgt ggctgctcgc acatccgtgg ccaggcaacg tccgtgagct cgagaacctg
      481 gtcgagcgtg cggccgtgct ggcgcgcgag gaccagatct cgctcgcgga cctgctgctc
      541 gacagcgccg ggccgggcgg ggcggggcaa agcgccacgg ccggcggcga ggacgccgag
      601 ttgccgttac aacaatatct cgaccgcgcc gccgagcgac ggatccgcgg ggcgctggcc
      661 cgctgcgagg gacgccgggc cgaggccgcc aggagcctcg gggtcgagcg gacgaccctg
      721 tatcgactga tgaagaaatt cgggatctcc gtcgacggcg agtgaagcga cgggggtgcg
      781 aacgcgcttg aatacgggcg cggcgcttcg tacgatgcgc ggatgcgaac tcttctcctg
      841 tggctgggcc tcggcgccgg cgcggcgctg gcggtggcat gcgacgacgc ggacagcgtg
      901 gattgcgagt gcgcggaggt cggctgcttc gcgccgatgt gtacgcgcct cgcgttcgtg
      961 ctcgccgagc cagtgtcacc gaagttcggt ggggtgagcg ctgccgacca gatgtgcgcg
     1021 cagcaggccc aggcggccgg gctgcccggg acgtactacg cgtggttggg ccacagcggc
     1081 ggcggcccgg cggaccgctt ctcgcgcgcg agcgtgccgt acacgctgcc cgatggcacg
     1141 ctgctcgccg ccgactggga cgcgctgacc cgcgagggcc cggacgctgc gatcgacatg
     1201 acggcggccg gcaagcgggt cgaggccgag gacgacgcgc gcgtgtggac cggcacgaac
     1261 gaggcgggcg acgcgaacac gtataataat gcgtcgaact attgctcgga ttggacgcgc
     1321 aacgtgatcg gggagttcgc ggtggtcggg ttcctgcgtg acaggagcaa gatcggcgcg
     1381 tgggcgctcg gtaacctcgt gccgtgcacg gggaaggggt atgtgtattg ctttcagcag
     1441 tgagaggctt tgacagctcc ttgatcgcgc tcgtctttcg ctccaaggtt gcgtcttact
     1501 tcacgaagtc gcgccaatgc tgagctcgcg ggtctccagg agtcgcgggc cggatctggc
     1561 gccagcactc gccccacatc accaatgtga taagggtggc ttgaata
//
LOCUS       DASWNJ010002061         3209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_352761, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002061 DASWNJ010000000
VERSION     DASWNJ010002061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352761"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VGB85_34325"
     CDS             complement(<1..435)
                     /locus_tag="VGB85_34325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139232.1"
                     /translation="MALGAGCAPKAATTAAPETAASSTPALDVAQPACARQAEEVEDA
                     LVRLADRYLWEEVERLVALAVAARSTWTDARACEAAARTALTTVARRWAEAGIGGGSQ
                     TFDLAQRALRSLETHFPGAKAELHHVFGRLEWAHATRVAGALG"
     gene            complement(471..2255)
                     /locus_tag="VGB85_34330"
     CDS             complement(471..2255)
                     /locus_tag="VGB85_34330"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0139233.1"
                     /translation="MEVTDRSTGPEPAVDATAVTLPPPSRPRAPEVAGASDDRTIDAP
                     TGGNKPAVADLAELEPGAVIARYRILGRLGAGGMGVVYRAHDGQLDRDVALKLLRVGE
                     DGTEGRTRMLREAKAAAKIRHPNVVTVYDAGEVFGRVFIAMELIDGVTLKAFFRGRKR
                     VWREVVEVMLGAGEGLAAAHAAGLVHRDFKPDNVLVETGGRVRVLDFGLARPVAESEG
                     PTMRGPSLTASDGPPVLRTLTQTGMLVGTPAYMAPEQHLCRGVDARSDQFAYCVTFFE
                     CLFGQRPFAGDTQAELTMNVVEGRLLPPKDRGDVPAEILAALQRGLATDPDDRHDSLA
                     DLLAQLRASLRRHAPGDRRGLLIAAGLMLPLVLGAAMYTTWAPEPTVDPQPAPERVVE
                     DLEVDRSITVPSGVSLAQERPGVTIGAPRTLPAAAFAELVAGVLPDGLDVETGPAGVR
                     VVGAGAGAWREPIQLLLSAIDVDNTRRPPGALVTQFTTPDGLCALYLLSVDQYAGCKT
                     EALASVSGVLRFGRSDDLRAVLVIGDGEAQAGVGELLRRAAAPKYREWCYVNTKMGDS
                     RHCYPTREACEKEATEWLDRKGHCKGVK"
     gene            2432..>3209
                     /locus_tag="VGB85_34335"
     CDS             2432..>3209
                     /locus_tag="VGB85_34335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139234.1"
                     /translation="MHDADTHAPSPAARELDASLAAALADVHMAIARAADEVDLMAAL
                     AGVLDRRAPARCELCLFSHDEHGAPATIAITVVRASGTTTRDATRATPVSAAPLASLW
                     LAAPNDALEIPDLDADPRVSAGDRQALTAYRAVTILPLHSHRQRSWQGYIRLLWTAPR
                     RSGAADHFVDRVLMQTVAAAIASRRTLRAHEAAIAETQALQAAQARLHAAASQAELLA
                     VVAEIAATHDASAAWLARVDPDDHGAPAWLEILATWGDAAV"
BASE COUNT          391 a         1364 c         1065 g          389 t
ORIGIN      
        1 gccgagcgcc ccggcgaccc gcgtcgcgtg ggcccactcg aggcgcccga acacgtggtg
       61 cagctccgcc ttcgcgcccg ggaagtgcgt ctccagcgat cgcagggccc gctgcgccag
      121 gtcgaaggtc tggctgccgc cgccgatccc cgcctcggcc cagcgccgcg cgaccgtcgt
      181 cagcgccgtg cgggccgccg cctcgcacgc gcgcgcgtcg gtccaggtgc tgcgcgccgc
      241 gaccgcgagc gcgaccagcc gctcgacctc ctcccacaga tagcggtccg ccaggcgcac
      301 cagcgcgtcc tcgacctcct cggcctggcg cgcgcacgcc ggctgggcga cgtccagcgc
      361 cggggtcgag ctcgccgccg tctccggcgc cgccgtcgtc gccgccttcg gcgcgcaacc
      421 tgccccgagc gccatggccg tcaggacatg tccacaaagc cagccgcgga tcacttgacg
      481 cccttgcagt ggcccttgcg gtccagccac tcggtcgcct ccttctcgca ggcctcgcgg
      541 gtcgggtaac agtgccggct gtcgcccatc ttggtgttga cgtagcacca ctcgcggtac
      601 ttcggcgccg ccgcgcgccg cagcagctcg ccgacgcccg cctgcgcctc gccgtcgccg
      661 atcaccagca ccgcgcgcaa gtcgtcgctg cgaccgaagc gcagcacccc gctgaccgac
      721 gccagcgcct cggtcttgca gcccgcgtac tggtcgaccg acagcaggta cagcgcgcac
      781 agcccgtcgg gggtcgtgaa ctgcgtcacc agggccccgg gcgggcgccg cgtattgtcg
      841 acgtcgatcg ccgacagcag cagctggatc ggctcgcgcc acgcgcccgc gccggcaccg
      901 accacgcgca cgcccgccgg ccccgtctcg acgtcgaggc cgtccggcag gacccccgcg
      961 acgagctcgg cgaaggccgc cgccggcagg gtccgcggcg ccccgatcgt cacacccggc
     1021 cgctcctgcg ccaggctgac gcccgacggc acggtaatac tgcggtcgac ctcgaggtcc
     1081 tctaccaccc gctcgggcgc cggctgcggg tccaccgtcg gctccggcgc ccacgtcgtg
     1141 tacatcgccg cgccgagaac cagcggcagc atcaggcccg ccgcgatcaa caggccacgc
     1201 cggtcgccgg gcgcgtgccg gcgcaggctc gcgcgcagct gggcgagcag gtcggcgagc
     1261 gagtcgtggc gatcgtcggg gtcggtcgcc aggccgcgct gcagcgccgc gaggatctcc
     1321 gccggcacgt cgccgcggtc cttcggcggc aacagccggc cctcgacgac gttcatcgtc
     1381 agctcggcct gcgtgtcgcc ggcgaacggc cgctgcccga acaggcactc gaagaaggtc
     1441 acgcagtacg cgaactgatc cgagcgcgcg tcgacgccgc ggcacaggtg ctgctccggg
     1501 gccatgtacg ccggcgtgcc gaccagcatg cccgtctgcg tcagcgtccg cagcaccggc
     1561 ggcccgtccg acgccgtcag gctcggcccc cgcatcgtcg gtccctccga ctccgcgacc
     1621 ggccgcgcga gcccgaagtc gagcacgcgc acgcgcccgc cggtctcgac gagcacgttg
     1681 tcgggcttga agtcccggtg caccaggccc gcggcgtggg ccgccgccag gccctcgccg
     1741 gcgccgagca tcacctcgac cacctcgcgc cacacccgct tgcgcccgcg aaagaacgcc
     1801 ttgagcgtga ccccgtcgat cagctccatc gcgatgaaca cccggccgaa cacctcgccc
     1861 gcgtcgtaca ccgtgacgac gttcgggtgg cggatcttgg ccgcggcctt ggcctcgcgc
     1921 agcatccgcg tgcgaccctc cgtgccgtcc tcgccgacgc gcagcagctt gagcgcgacg
     1981 tcgcggtcga gctgcccgtc gtgggcgcga tacaccaccc ccatgccgcc ggcccccagt
     2041 cgaccgagga tccggtagcg agcgatcacc gcgcccggct cgagctccgc gaggtcggcg
     2101 accgccggct tgttgccgcc ggtcggcgcg tcgatcgtac ggtcgtcgct ggcccccgcc
     2161 acctcgggtg cgcgcggcct cgatggcggc ggcagggtca ccgccgtcgc gtcgaccgcg
     2221 ggctcgggcc ccgtgctcct gtccgtcacc tccatccgcc gcgaatttac ccgtctcgcg
     2281 gcccctgcga caacgccccg gcggattatc gtcgttcatc gcccacggac ccggggccaa
     2341 acggccgtgg gccggcctgc gaccgcgcgc cgccgcgccg tcgggtccgg cggacgcgcg
     2401 gcccggcatc gcctaagctg gcccgcccag gatgcacgac gcggacacgc acgccccctc
     2461 gccggccgct cgcgagctcg acgcctccct ggcggccgcc ctcgccgacg tgcacatggc
     2521 gatcgcacgc gcggccgacg aggtcgatct catggccgcc ctcgccggcg tcctcgatcg
     2581 tcgcgccccg gcccgctgcg agctgtgcct gttctcccac gacgagcacg gcgcccccgc
     2641 gacgatcgcc atcaccgtcg tccgcgccag cggcacgacc acccgcgacg ccacgcgcgc
     2701 gacccccgtc tcggccgcgc cgctcgcctc gctgtggctc gccgccccga acgacgcgct
     2761 cgagatcccc gacctcgacg ccgacccgcg cgtgtccgcc ggcgaccgcc aggccctcac
     2821 ggcgtaccgc gccgtcacga tcctgccgct gcacagccac cgccagcgca gctggcaggg
     2881 ctacatccgc ctgctgtgga ccgcgccgcg ccgctccggc gcggccgacc acttcgtcga
     2941 tcgcgtgctc atgcagaccg tcgccgccgc gatcgccagc cgtcgcacgc tgcgcgccca
     3001 cgaggccgcg atcgccgaga cgcaggcgct gcaggccgcc caggcccggc tgcacgccgc
     3061 cgcctcgcag gccgagctgc tcgcggtcgt cgccgagatc gcagcgaccc atgacgccag
     3121 cgccgcctgg ctcgcccgcg tcgaccccga cgaccacggg gccccggcct ggctcgagat
     3181 cctcgccacc tggggcgacg ccgccgtgc
//
LOCUS       DASWNJ010002062         3965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nannocystis sp. isolate SMAG_U3411 k141_352788, whole
            genome shotgun sequence.
ACCESSION   DASWNJ010002062 DASWNJ010000000
VERSION     DASWNJ010002062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959230
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nannocystis sp. (soil metagenome)
  ORGANISM  Nannocystis sp.
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:25:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,871
            CDSs (total)                      :: 6,833
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Nannocystis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352788"
                     /isolate="SMAG_U3411"
                     /isolation_source="soil"
                     /db_xref="taxon:1962667"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VGB85_34340"
     CDS             <1..250
                     /locus_tag="VGB85_34340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0139235.1"
                     /translation="RLILLDLNMPRMNGIEFLRELRADPALRSSSVVVLTTSNEERDK
                     IEAYQFNVAGYLLKPVTFGNFVDVMAALDKYWTLVEMP"
     gene            247..1728
                     /locus_tag="VGB85_34345"
     CDS             247..1728
                     /locus_tag="VGB85_34345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0139236.1"
                     /translation="MSATDLKLPVRVFVIDDDEVDREIVRHLLPRAGIEAEVLEADDP
                     AVALATLREQGTTFVELIVLDYHFPREDGLAVLRALRELDTITPVIVLTGQDDVALAV
                     ELMKNGAADYIPKGALTSERLGRSVRHALRLRGLDLAARAAQEALRASEEFNRRVLEA
                     SADCIKVLDLDGRLVSMSAGGHRLMQIPEGTSMAGKLWLEFWSEDQQVAAMAAFTEAR
                     SGGVGHFVGHCAATSGATLWWDVVISPIQGAGGLPERILAISRDVTKQKRQAEFEQQL
                     IGIVSHDLRNPIAAMIMGASLIKLKTTPDSPLASTAGRIVKSGERATRLIRDLLDFTQ
                     VRLGGGVPIERRAADIHVVCAQAVEEIALNYPDRIIIREDEGDGHGAWDADRLAQVVG
                     NLVRNAVSYGEPGAPITVSSVGRATTVEIRVHNHGKPIAPDVLPTLFEPFKRGDRRHD
                     PERSVGLGLFIVREIVAAHGGTVEVCSEVEQGTCFSVTLPRGA"
     gene            1864..3660
                     /locus_tag="VGB85_34350"
     CDS             1864..3660
                     /locus_tag="VGB85_34350"
                     /inference="COORDINATES: protein motif:HMM:NF015055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-arginine deiminase family protein"
                     /protein_id="HEY0139237.1"
                     /translation="MRRLGLVLPVICGLLACKEDDAAEGTSAGEDTAVTTGSDTDPDP
                     TGSATQASDPSGAEGSATGTGSATEVTTTTTESGGTTSDDTTTDETTEEPPPAIIWSF
                     DDVFGRANVDDDDDNGKEDWLDARFDGDNELAMQVLRGATLDLLPAGARVRLTLGGPG
                     DALRAWIGDELVLGSTQGAPILEHVLTPPGGDVTLAWEFLGHNTKAELTITWLDEQDA
                     EVESVVVDVRASPLITNHHLQPAEHAWVVAVSGGQSYNNSSMVQAMQAVLGDKLTRVP
                     EDDYDYDVWIQDEFEWATARSSDGGRMDVVIDSIRNRGLDPMPEQTLVGPDYVAQTWG
                     EGPKTSYDSFGNLDASPPVTVDGVFYPFGRIYYGRDNGEGINAKMAEFLASQEVQKPF
                     ELDTTWLCVGHVDEYSTIVPDPSAPKGFRLLLADVPSMYQLLDAAPKATNLPLYNKDY
                     GYGSIGEIVGDAGLRAYNEDIQADHLDGIRAVFKKELGLDDADIISVPTIFESINNCG
                     AVALTPGTVNLAMFNFAGEPTHAFVPDPFLRATVGDQASDPIIADYKARMPKDLVLHF
                     VDNWNVYHVNLGEVHCGTNVQRAPLANWWEEN"
     gene            3663..>3965
                     /locus_tag="VGB85_34355"
     CDS             3663..>3965
                     /locus_tag="VGB85_34355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0139238.1"
                     /translation="MSTLHRLLALALALISGSAPALLAGSAPALLASCAPDSSDEPEA
                     AESRVPFDAADASVASGEPALGEWQAWLSGELARAEQERPAAVAQLRAVAPVATRAG"
BASE COUNT          645 a         1407 c         1346 g          567 t
ORIGIN      
        1 caggttgatc ctgctcgacc tcaacatgcc gcgcatgaac ggcatcgagt tcctgcgcga
       61 gctgcgggcc gacccggcgc tgcgaagctc gtcggtcgtc gtgctgacga cctcgaacga
      121 ggagcgcgac aagatcgagg cgtaccagtt caacgtcgcc ggctacctgc tcaagccggt
      181 caccttcggc aacttcgtcg acgtgatggc ggcgctcgac aaatactgga ccctggtaga
      241 gatgccgtga gcgcgacgga cctcaagctg cccgtccgcg tgttcgtcat cgacgacgac
      301 gaggtcgacc gtgagatcgt gcggcacctg ctgccgcgcg ccggcatcga ggcggaggtg
      361 ctcgaggcgg acgacccggc ggtcgcgctg gcgaccctgc gcgagcaggg caccaccttc
      421 gtcgagctga tcgtgctcga ctatcacttc ccgcgcgagg acgggctcgc ggtcctgcgc
      481 gcgctgcgag agctcgacac gatcacgccg gtcatcgtgt tgaccggaca ggacgacgtc
      541 gcgctcgccg tcgagctgat gaaaaacggc gccgccgact acatccccaa gggcgcgctg
      601 acctcggagc ggctcggtcg cagcgtccgt cacgcgctgc gcctgcgcgg gctcgacctc
      661 gccgcccgcg ccgcgcagga ggcgttgcgc gcgagcgagg agttcaaccg ccgcgtgctc
      721 gaggcgagcg ccgactgcat caaggtgctc gacctcgacg gtcgtctggt gtcgatgagc
      781 gccggcggtc accgcctgat gcagatcccc gaggggacct cgatggcggg caagctctgg
      841 ctcgagttct ggtcggagga ccagcaggtg gcggccatgg ccgcgttcac ggaggcgcgc
      901 agcggcggcg tcggccactt cgtcggtcac tgcgcggcga cctcgggcgc gacgctgtgg
      961 tgggacgtcg tcatctcgcc gatccagggc gcgggcggcc tcccggagcg catcttggcg
     1021 atctcgcgcg acgtcaccaa gcaaaagcgc caggcggagt tcgaacagca gctgatcggc
     1081 atcgtcagcc acgacctgcg caacccgatc gcagcgatga tcatgggcgc ctcgctgatc
     1141 aagctcaaga ccacgcccga ctcgccgctg gccagcaccg ccgggcggat cgtcaagagc
     1201 ggcgaacgtg cgacccgcct gatccgcgac ctcctcgact tcacgcaggt gcgcctcggc
     1261 ggcggcgtgc cgatcgagcg ccgcgcggcc gatatccacg tcgtctgcgc ccaggccgtc
     1321 gaggagatcg cgctcaacta ccccgaccgc atcatcatcc gcgaggacga gggcgacgga
     1381 cacggcgcct gggacgccga ccgcctcgcg caggtcgtcg gcaacctggt tcgcaacgcc
     1441 gtcagctacg gcgagcctgg cgcaccgatc accgtgtcca gcgtcggtcg cgcgacgacg
     1501 gtcgagatcc gggtgcataa tcacggcaag ccgatcgcac ccgatgtgct gccgaccctg
     1561 ttcgaaccgt tcaagcgcgg cgaccgtcgg cacgaccccg agcgcagcgt cggcctcggc
     1621 ctgttcatcg tccgcgagat cgtggcggcc cacggcggca ccgtggaggt ctgctccgaa
     1681 gtcgagcaag ggacgtgctt ctcggtcacg ctgccccgcg gtgcttgatc gcgttcgcgt
     1741 cgacagtgca gcggggttca cagcatcatc cgtcgtggag cgaggggtgg cgagccctca
     1801 ggtgattgtc ctcaggtggc tcgctgttgc gagcccatag ctccagggat atcgtccggg
     1861 acgatgcgaa gactcggtct ggtcctcccg gtcatctgcg gacttctcgc ctgcaaggag
     1921 gacgacgccg ccgagggcac cagtgccggt gaggacaccg ccgtcacgac cggcagtgac
     1981 accgaccccg atccgaccgg cagcgccacg caggcgagcg acccgagcgg cgccgagggc
     2041 agcgcgacgg gcacgggcag cgcgacggag gtcacgacca cgacgaccga gagcggtggg
     2101 accacgagcg acgacacgac cacggacgag acgaccgagg agccgccgcc ggcgatcatc
     2161 tggagcttcg acgacgtgtt cgggcgggcc aacgtcgacg atgacgacga caacggcaag
     2221 gaggactggc tcgacgcgcg cttcgacggc gacaacgagc tggcgatgca ggtgttgcgc
     2281 ggcgcgacgc tcgacctgtt gccggccggc gcccgcgtgc gcctgaccct cggcggcccc
     2341 ggcgacgcgc tgcgggcgtg gatcggtgac gagctcgtgc tcggctcgac ccagggcgcg
     2401 ccgatcctcg agcacgtgct gacgccgccg ggcggcgacg tcacgctcgc gtgggagttc
     2461 ctcggtcaca atacgaaggc cgagctgacg atcacctggc tcgacgagca ggacgccgag
     2521 gtcgagtccg tcgtcgtcga cgttcgtgcc tcgccgctga tcaccaacca tcacctgcag
     2581 ccggccgagc acgcgtgggt cgtcgcggtg tcgggcgggc agagctacaa caacagctcg
     2641 atggtccagg cgatgcaggc ggtgctcggc gacaagctga cccgggtgcc cgaggacgac
     2701 tacgactacg acgtgtggat ccaggacgag ttcgagtggg cgaccgcgcg cagcagcgac
     2761 ggtggccgca tggatgtcgt catcgactcg atccgcaacc gcgggctcga cccgatgccc
     2821 gagcagacgc tcgtcgggcc ggactacgtc gcgcagacct ggggcgaggg gccgaagacc
     2881 tcgtacgact cgttcggcaa cctcgacgcc tcgccgccgg tcaccgtcga cggtgtcttt
     2941 taccccttcg gccggatcta ctacggccgc gacaacggcg agggcatcaa cgccaagatg
     3001 gccgagttcc tggcctcgca ggaggtccag aagcccttcg agctcgacac cacctggctg
     3061 tgtgtcggcc acgtcgacga gtacagcacg atcgtgcccg acccctcggc gccgaagggc
     3121 tttcgcctgc tgctcgccga cgtgccctcg atgtatcagc tcctcgacgc cgccccgaag
     3181 gcgaccaacc tgccgctcta caacaaggac tacggctacg gcagcatcgg cgagatcgtc
     3241 ggcgacgccg gcctgcgcgc ctacaacgag gacatccagg ccgaccacct cgacggcatc
     3301 cgcgcggtct ttaaaaagga gctcgggctc gacgatgccg acatcatctc ggtgccgacg
     3361 atcttcgagt cgatcaacaa ctgcggcgcg gtcgcgctga ccccgggcac cgtcaacctg
     3421 gcgatgttca acttcgcggg cgagccgacc cacgccttcg tgcccgaccc gttcctgcgc
     3481 gcgaccgtcg gcgaccaggc gtcggacccg atcatcgccg actacaaggc ccggatgccc
     3541 aaggacctcg tgctgcactt cgtcgacaac tggaacgtct atcacgtgaa cctcggcgag
     3601 gttcactgcg gcaccaacgt ccagcgcgcg ccgctcgcga actggtggga ggagaactga
     3661 tcatgagcac gcttcaccga ctcctcgcgc tcgcgctggc gctcatctcc ggcagcgctc
     3721 ccgcgctgct cgccggcagc gctcccgcgc tgctcgccag ctgcgccccc gattccagcg
     3781 acgaacccga ggccgccgag tcgcgcgtgc ccttcgacgc cgccgacgcc tccgttgcat
     3841 ccggtgagcc ggcgctcggg gagtggcagg cgtggctgtc gggcgagctc gcccgcgccg
     3901 aacaggagcg gcccgccgcg gtcgcccagc tgcgcgcggt tgcgcccgtt gcgacccgcg
     3961 ccggc
//
